bluuebunny/crossref_metadata_2025 huggingface dataset:
The Legacy of Peter Wynn
Authors: Brezinski Claude , Norman F. Alexander , Redivo-Zaglia Michela
Published: May 2021

After the death of Peter Wynn in December 2017, manuscript documents he left came to our knowledge. They concern continued fractions, rational (Padé) approximation, Thiele interpolation, orthogonal polynomials, moment problems, series, and abstract algebra. The purpose of this paper is to analyze them and to make them available to the mathematical community. Some of them are in quite good shape, almost finished, and ready to be published by anyone willing to check and complete them. Others are rough notes, and need to be reworked. Anyway, we think that these works are valuable additions to the literature on these topics and that they cannot be left unknown since they contain ideas that were never exploited. They can lead to new research and results. Two unpublished papers are also mentioned here for the first time.
On Model Order Reduction of Interconnect Circuit Network: A Fast and Accurate Method
Authors: Wang Xinsheng , Fan Shimin , Dai Ming-Zhe , Zhang Chengxi
Published: May 2021

The time cost in integrated circuit simulation is an important consideration in the design. This paper investigates the model order reduction of interconnect circuit networks to facilitate numerical analysis. A novel fast and accurate time reduced order model is proposed to simplify the interconnection network structure analysis and perform a fast simulation. The novelty of this study is the use of the power function sum to extend the approximate function to replace the original system’s state function. We give several simulations to verify the effectiveness of the algorithm. The innovation of this model is due to its use of the approximate function of power series expansion to replace the state function of the original system.
Analysis of Single-Server Multi-Class Queue with Unreliable Service, Batch Correlated Arrivals, Customers Impatience, and Dynamical Change of Priorities
Authors: Dudin Alexander , Dudina Olga , Dudin Sergei , Samouylov Konstantin
Published: May 2021

A single-server non-pre-emptive priority queueing system of a finite capacity with many types of customers is analyzed. Inter-arrival times can be correlated and batch arrivals are allowed. Possible unreliability of the server, implying the loss of a customer or the necessity of its service from the early beginning or some phase of the service, is taken into account. Initial priorities provided to various types of customers at the arrival moment can be varied (increased or decreased) after the random amount of time during the customer stay in the buffer. Such a type of queues arises in the modeling operation of various emergency care systems, information, and perishable goods delivering systems, etc. The stationary behavior of the system is described by the finite state multi-dimensional continuous-time Markov chain with the upper-Hessenberg block structure of the generator. The stationary distribution of the system states and some important characteristics of the system are calculated. The presented numerical examples illustrate opportunities to quantitatively evaluate the impact of the buffer capacity and customers’ mean arrival rate on the most important characteristics of the system. The possibility of solving optimization problems is briefly shown.
Geometric Algebra Framework Applied to Symmetrical Balanced Three-Phase Systems for Sinusoidal and Non-Sinusoidal Voltage Supply
Authors: Montoya Francisco G. , Baños Raúl , Alcayde Alfredo , Arrabal-Campos Francisco Manuel , Roldán Pérez Javier
Published: May 2021

This paper presents a new framework based on geometric algebra (GA) to solve and analyse three-phase balanced electrical circuits under sinusoidal and non-sinusoidal conditions. The proposed approach is an exploratory application of the geometric algebra power theory (GAPoT) to multiple-phase systems. A definition of geometric apparent power for three-phase systems, that complies with the energy conservation principle, is also introduced. Power calculations are performed in a multi-dimensional Euclidean space where cross effects between voltage and current harmonics are taken into consideration. By using the proposed framework, the current can be easily geometrically decomposed into active- and non-active components for current compensation purposes. The paper includes detailed examples in which electrical circuits are solved and the results are analysed. This work is a first step towards a more advanced polyphase proposal that can be applied to systems under real operation conditions, where unbalance and asymmetry is considered.
The Application of Control Materials for Ongoing Quality Management of Next-Generation Sequencing in a Clinical Genetic Laboratory
Authors: Min Young-Kyu , Park Kyung-Sun
Published: May 2021

Next-generation sequencing (NGS) has played an important role in detecting genetic variants with pathologic and therapeutic potential. The advantages of NGS, such as high-throughput sequencing capacity and massively parallel sequencing, have a significant impact on realization of genetic profiling in clinical genetic laboratories. These changes have enabled clinicians to execute precision medicine in diagnosis, prognosis, and treatment for patients. However, to adapt targeted gene panels in diagnostic use, analytical validation and ongoing quality control should be implemented and applied with both practical guidelines and appropriate control materials. Several guidelines for NGS quality control recommend usage of control materials such as HapMap cell lines, synthetic DNA fragments, and genetically characterized cell lines; however, specifications or applications of such usage are insufficient to guideline method development. This review focuses on what factors should be considered before control material selection for NGS assay and practical methods of how they could be developed in clinical genetic laboratories. This review also provides the detailed sources of critical information related to control materials.
Modern Technologies in the Rehabilitation of Patients with Multiple Sclerosis and Their Potential Application in Times of COVID-19
Authors: Zasadzka Ewa , Trzmiel Tomasz , Pieczyńska Anna , Hojan Katarzyna
Published: May 2021

Background and Objectives: The COVID-19 pandemic required the adoption of new technologies to improve access to healthcare at an unprecedented speed, as social distancing became mandatory. The aim of this systematic review was to analyze the effectiveness of using new technologies in the rehabilitation of multiple sclerosis (MS) patients and discuss their potential role during the COVID-19 pandemic. Material and Methods: The studies were identified by searching two online databases—PUBMED and Web of Science. Combinations of the key words “Multiple sclerosis” and “e-health”; “Multiple sclerosis” and “virtual reality”; “Multiple sclerosis” and “telerehabilitation”; “Multiple sclerosis” and “new technologies”; “Multiple sclerosis” and “tele-exercise” were used to find suitable publications. Results: A total of 17 studies were included. Although the overall number of participants in all the studies was 904, two of the studies were conducted on the same group. Thus, a total of 854 participants were involved in the studies included. All participants were diagnosed with MS. In 10 studies, participants had to be diagnosed according to the McDonald criteria. Of the included studies: five involved intervention at participants’ home, six were conducted using Xbox Kinect, and seven studies reported no adverse outcomes. Conclusion: The review proves telerehabilitation to be an effective motivational tool to restore and maintain both physical and cognitive function in patients with MS. Remote communication technologies seem to be measures of high effectiveness in rehabilitating and supporting MS patients especially during the COVID-19 pandemic, as the traditional rehabilitation option is less accessible or in some cases inaccessible for these patients.
A Simulation Case Study of Knee Joint Compressive Stress during the Stance Phase in Severe Knee Osteoarthritis Using Finite Element Method
Authors: Fukaya Takashi , Mutsuzaki Hirotaka , Aoyama Toshiyuki , Watanabe Kunihiro , Mori Koichi
Published: May 2021

Background and Objectives: Medial knee osteoarthritis is known to increase the mechanical load on the medial compartment of the knee joint during walking; however, it is not visually understood how much the mechanical load increases nor where in the medial compartment of the knee joint that load is focused. Therefore, we conducted a simulation study to determine the location and amount of the mechanical load in the medial compartment of the knee joint during the stance phase. Materials and Methods: Subject was a patient with right medial knee osteoarthritis. Computed tomography imaging and gait analysis were performed on subject. The CT image of the right knee was calculated using finite element analysis software. Since this software can set the flexion angle arbitrarily while maintaining the nonuniform material properties of the bone region, the model is constructed by matching the knee joint extension image obtained by CT to the loading response phase of gait analysis. The data of muscle exertion tension and vertical ground reaction force were inserted into the knee joint model created from the computed tomography-based finite element method, and the knee joint compressive stress was calculated. Results: With regard to compressive stress, the tibia showed high stress at 4.10 to 5.36 N/mm2. The femur showed high stress at 4.00 to 6.48 N/mm2. The joint compressive stress on the medial compartment of the knee joint was found to concentrate on the edge of the medial tibial condyle in the medial knee osteoarthritis subject. Conclusions: The measurement method of knee joint compressive stress by computed tomography-based finite element method can visually be a reliable method of measuring joint compressive stress in the medial knee osteoarthritis. This reflects the clinical findings because concentration of stress on the medial knee joint was observed at the medial osteophyte.
Subclinical Changes in Cardiac Functional Parameters as Determined by Cardiovascular Magnetic Resonance (CMR) Imaging in Sleep Apnea and Snoring: Findings from UK Biobank
Authors: Curta Adrian , Hetterich Holger , Schinner Regina , Lee Aaron M. , Sommer Wieland , Aung Nay , Sanghvi Mihir M. , Fung Kenneth , Lukaschuk Elena , Cooper Jackie A. , Paiva José Miguel , Carapella Valentina , Neubauer Stefan , Piechnik Stefan K. , Petersen Steffen E.
Published: May 2021

Background and Objectives: Obstructive sleep apnea (OSA) is a common disorder with an increased risk for left ventricular and right ventricular dysfunction. Most studies to date have examined populations with manifest cardiovascular disease using echocardiography to analyze ventricular dysfunction with little or no reference to ventricular volumes or myocardial mass. Our aim was to explore these parameters with cardiac MRI. We hypothesized that there would be stepwise increase in left ventricular mass and right ventricular volumes from the unaffected, to the snoring and the OSA group. Materials and Methods: We analyzed cardiac MRI data from 4978 UK Biobank participants free from cardiovascular disease. Participants were allocated into three cohorts: with OSA, with self-reported snoring and without OSA or snoring (n = 118, 1886 and 2477). We analyzed cardiac parameters from balanced cine-SSFP sequences and indexed them to body surface area. Results: Patients with OSA were mostly males (47.3% vs. 79.7%; p < 0.001) with higher body mass index (25.7 ± 4.0 vs. 31.3 ± 5.3 kg/m²; p < 0.001) and higher blood pressure (135 ± 18 vs. 140 ± 17 mmHg; p = 0.012) compared to individuals without OSA or snoring. Regression analysis showed a significant effect for OSA in left ventricular end-diastolic index (LVEDVI) (β = −4.9 ± 2.4 mL/m²; p = 0.040) and right ventricular end-diastolic index (RVEDVI) (β = −6.2 ± 2.6 mL/m²; p = 0.016) in females and for right ventricular ejection fraction (RVEF) (β = 1.7 ± 0.8%; p = 0.031) in males. A significant effect was discovered in snoring females for left ventricular mass index (LVMI) (β = 3.5 ± 0.9 g/m²; p < 0.001) and in males for left ventricular ejection fraction (LVEF) (β = 1.0 ± 0.3%; p = 0.001) and RVEF (β = 1.2 ± 0.3%; p < 0.001). Conclusion: Our study suggests that OSA is highly underdiagnosed and that it is an evolving process with gender specific progression. Females with OSA show significantly lower ventricular volumes while males with snoring show increased ejection fractions which may be an early sign of hypertrophy. Separate prospective studies are needed to further explore the direction of causality.
Differential Antimicrobial Effect of Essential Oils and Their Main Components: Insights Based on the Cell Membrane and External Structure
Authors: Andrade-Ochoa Sergio , Chacón-Vargas Karla Fabiola , Sánchez-Torres Luvia Enid , Rivera-Chavira Blanca Estela , Nogueda-Torres Benjamín , Nevárez-Moorillón Guadalupe Virginia
Published: May 2021

The biological activity of essential oils and their major components is well documented. Essential oils such as oregano and cinnamon are known for their effect against bacteria, fungi, and even viruses. The mechanism of action is proposed to be related to membrane and external cell structures, including cell walls. This study aimed to evaluate the biological activity of seven essential oils and eight of their major components against Gram-negative and Gram-positive bacteria, filamentous fungi, and protozoans. The antimicrobial activity was evaluated by determination of the Minimal Inhibitory Concentration for Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium, Shigella sonnei, Aspergillus niger, Aspergillus ochraceus, Alternaria alternata, and Fusarium oxysporium, the half-maximal inhibitory concentration (IC50) for Trypanosoma cruzi and Leishmania mexicana, and the median lethal dose (LD50) for Giardia lamblia. Results showed that oregano essential oil showed the best antibacterial activity (66–100 µg/mL), while cinnamon essential oil had the best fungicidal activity (66–116 µg/mL), and both showed excellent antiprotozoal activity (22–108 µg/mL). Regarding the major components, thymol and carvacrol were also good antimicrobials (23–200 µg/mL), and cinnamaldehyde was an antifungal compound (41–75 µg/mL). The major components were grouped according to their chemical structure as phenylpropanoids, terpenoids, and terpinenes. The statistical analysis of the grouped data demonstrated that protozoans were more susceptible to the essential oils, followed by fungi, Gram-positive bacteria, and Gram-negative bacteria. The analysis for the major components showed that the most resistant microbial group was fungi, which was followed by bacteria, and protozoans were also more susceptible. Principal Component Analysis for the essential oils demonstrated the relationship between the biological activity and the microbial group tested, with the first three components explaining 94.3% of the data variability. The chemical structure of the major components was also related to the biological activity presented against the microbial groups tested, where the three first principal components accounted for 91.9% of the variability. The external structures and the characteristics of the cell membranes in the different microbial groups are determinant for their susceptibility to essential oils and their major components
Rational Design Method Based on Techno-Economic Principles for Integration of Organic/Organic Pervaporation with Lipase Catalyzed Transesterification
Authors: Van Hecke Wouter , Debergh Pieterjan , Khan Mohammed Nazeer , Van Dael Miet
Published: May 2021

An engineering foundation is developed in this manuscript to allow the rational design of enzymatic transesterifications integrated with organic–organic pervaporation for the removal of methanol. In the first part, enzyme kinetics are elucidated for the solventless transesterification of two monoterpene alcohols with methyl acetate catalyzed by Novozym 435. Nonlinear regression revealed that three parameters (enzyme loading, forward and backward second-order reaction rate) sufficed to describe the entire conversion as a function of time. In the second part, a mathematical model for acetate ester production, integrated with organic–organic pervaporation, was developed based on a set of ordinary differential equations. To this end, empirical formulae for the pervaporation performance (of a PERVAP 2255-30 membrane and a standard HybSi® membrane) were established, relating methyl acetate and methanol flux to the methanol concentration in the reactor. The resulting digital twin, “PervApp”, allows us to study the influence of the key design parameters “enzyme loading” and “membrane surface” on, e.g., catalyst productivity. Finally, a techno-economic assessment is made for an annual production of 100 tons of geranyl acetate. The described methodology allows producers to shift from laborious, expensive and often disappointing trial-and-error approaches to the rational design of such integrated units.
Assessment of an Anaerobic Membrane Bioreactor (AnMBR) Treating Medium-Strength Synthetic Wastewater under Cyclical Membrane Operation
Authors: Uman Ahmet E. , Bair Robert A. , Yeh Daniel H.
Published: May 2021

A lab-scale (6.2 L) anaerobic membrane bioreactor combined with a tubular, cross-flow, PVDF ultrafiltration membrane was developed and operated to assess the long-term fouling behavior of a cyclically operated anaerobic membrane bioreactor (AnMBR). The AnMBR was operated at 35 ± 1 °C for 200 days with a synthetic influent of 501 mg·L−1 COD to mimic municipal wastewater. The system exhibited high treatment performance with an average COD removal efficiency of 86.5 ± 6.4% (n = 20) and an average permeate COD concentration of 63.9 ± 31.1 mg·L−1. A clear permeate with an average turbidity of 0.6 ± 0.2 NTU, was achieved. Permeate TN and TP concentrations were 22.7 ± 5.1 mg·L−1 and 6.9 ± 2.0 mg·L−1 corresponding to removal efficiencies of 20.6% and 49.3%, respectively, likely due to membrane rejection of particulate, colloidal, and organic fractions. A stable membrane flux of 4.3 L.m−2.h−1 (LMH) was maintained for 183 days without gas-lift, gas sparge, or chemical cleaning. Cyclical operation with frequent relaxation (60 s for every 30 min of the permeate production run) and periodic permeate backwash (15 s for every 186 min) maintained stable membrane operation with an average TMP of 0.25 bar and a fouling rate of 0.007 kPa/h for the entire operating period. The comparison revealed frequent backwashing and relaxation is a sustainable strategy for operation of the AnMBR.
Investigation of Energy-Absorbing Properties of a Bio-Inspired Structure
Authors: Dubicki Adrian , Zglobicka Izabela , Kurzydłowski Krzysztof J.
Published: May 2021

Numerous engineering applications require lightweight structures with excellent absorption capacity. The problem of obtaining such structures may be solved by nature and especially biological structures with such properties. The paper concerns an attempt to develop a new energy-absorbing material using a biomimetic approach. The lightweight structure investigated here is mimicking geometry of diatom shells, which are known to be optimized by nature in terms of the resistance to mechanical loading. The structures mimicking frustule of diatoms, retaining the similarity with the natural shell, were 3D printed and subjected to compression tests. As required, the bio-inspired structure deformed continuously with the increase in deformation force. Finite element analysis (FEA) was carried out to gain insight into the mechanism of damage of the samples mimicking diatoms shells. The experimental results showed a good agreement with the numerical results. The results are discussed in the context of further investigations which need to be conducted as well as possible applications in the energy absorbing structures.
Deposition of Arsenic from Nitric Acid Leaching Solutions of Gold–Arsenic Sulphide Concentrates
Authors: Karimov Kirill , Rogozhnikov Denis , Kuzas Evgeniy , Dizer Oleg , Golovkin Dmitry , Tretiak Maksim
Published: May 2021

At present, the processing of refractory gold–arsenic sulphide concentrates is becoming more relevant due to the depletion of rich crude ore reserves. In the process of the nitric acid leaching of arsenic sulphide minerals, solutions are formed containing 20–30 g/L of arsenic (III). Since market demand for arsenic compounds is limited, such solutions are traditionally converted into poorly soluble compounds. This paper describes the investigation of precipitating arsenic sulphide from nitric acid leaching solutions of refractory sulphide raw materials of nonferrous metals containing iron (III) ions using sodium hydrosulphide with a molar ratio of NaHS/As = 2.4–2.6, which is typical for pure model solutions without oxidants. The work studied the effect of temperature, the pH of the solution and the consumption of NaHS and seed crystal on this process. The highest degree of precipitation of arsenic (III) sulphide (95–99%) from nitric acid leaching solutions containing iron (III) ions without seed occurs with a pH from 1.8 to 2.0 and a NaHS/As molar ratio of 2.8. The introduction of seed crystal significantly improves the precipitation of arsenic (III) sulphide. An increase in seed crystal consumption from 0 to 34 g/L in solution promotes an increase in the degree of transition of arsenic to sediment from 36.2 to 98.1% at pH = 1. According to SEM/EDS and XRF sediment data, from the results of experiments on the effect of As2S3 seed crystal consumption, acidity and molar ratio of NaHS/As on the precipitation of arsenic (III) sulphide and the Fetotal/Fe2+ ratio in the final solution, it can be concluded that the addition of a seed accelerates the crystallisation of arsenic (III) sulphide by increasing the number of crystallisation centres; as a result, the deposition rate of As2S3 becomes higher. Since the oxidation rate of sulphide ions to elemental sulphur by iron (III) ions does not change significantly, the molar ratio of NaHS/As can be reduced to 2.25 to obtain a precipitate having a lower amount of elemental sulphur and a high arsenic content similar to that precipitated from pure model solutions.
Model Development to Study Uncertainties in Electric Arc Furnace Plants to Improve Their Economic and Environmental Performance
Authors: Arzpeyma Niloofar , Alam Moudud , Gyllenram Rutger , Jönsson Pär G.
Published: May 2021

A statistical model is developed in order to simulate the melt composition in electric arc furnaces (EAFs) with respect to uncertainties in (1) scrap composition, (2) scrap weighing and (3) element distribution factors. The tramp element Cu and alloying element Cr are taken into account. The model enables simulations of a charge program as well as backwards estimations of the element concentrations and their variance in scrap. In the backwards calculation, the maximum likelihood method is solved by considering three cases corresponding to the involved uncertainties. It is shown that the model can estimate standard deviations for elements so that the real values lie within the estimated 95% confidence interval. Moreover, the results of the model application in each target product show that the estimated scrap composition results in a melt composition, which is in good agreement with the measured one. The model can be applied to increase our understanding of scrap chemical composition and lower the charged material cost and carbon footprint of the products.
Effects of Various Processing Methods on the Metabolic Profile and Antioxidant Activity of Dendrobium catenatum Lindley Leaves
Authors: Liu Jing-Jing , Liu Zhen-Peng , Zhang Xin-Feng , Si Jin-Ping
Published: May 2021

The metabolite profiles and antioxidant activity of Dendrobium catenatum Lindley leaf, a new functional ingredient for food product development, were evaluated in samples that had been prepared using various methods, including freeze-drying, hot-air drying, rolling before drying, steaming before drying, steaming and rolling before drying, and drying at 100, 80, and 60 °C. The concentrations of polysaccharides and flavonoids, as well as the antioxidant capacity of each sample, were determined. Furthermore, two nucleosides, four amino acids, one monoaromatic compound, and eight flavonoids were identified in dried leaves using high-performance liquid chromatography–diode array detector–electrospray ionization–multistage mass spectrometry (HPLC-DAD-ESI-MSn) and ultraviolet (UV) spectral analyses. The content of polar compounds such as cytidylic acid, arginine, tyrosine, and hydroxybenzoic acid hexose increased dramatically during hot-air-drying and rolling-before-drying treatments, while flavonol C-glycosides remained stable throughout the various treatments and drying temperatures. Rolling before drying at 100 °C was identified as the most suitable process when manufacturing tea products from D. catenatum leaves. This process resulted in a high-antioxidant-activity and visually appealing tea. This report details a potential strategy that should be applied in the manufacturing processes of high-quality products from D. catenatum leaves.
Integrating Resonator to Enhance Magnetometer Microelectromechanical System Implementation with ASIC Compatible CMOS 0.18 μm Process
Authors: Lin Chih-Hsuan , Song Chao-Hung , Wen Kuei-Ann
Published: May 2021

In this study, a multi-function microelectromechanical system (MEMS) was integrated with a MEMS oscillator, using the resonant frequency oscillation characteristics of the oscillator to provide the Lorentz current of the magnetometer to enhance a large dynamic range of reading, which eliminates the off-chip clock and current generator. The resonant frequency can be adjusted by adjusting the bias voltage of the oscillator to further adjust the sensitivity of the magnetometer. With the mechanical Q value characteristic, a great dynamic range can be achieved. In addition, using the readout circuit of the nested chopper and correlated double-sampling (CDS) to reduce the noise and achieve a smaller resolution, the calibration circuit compensates for errors caused by the manufacturing process. The frequency of the tuning range of the proposed structure is 17,720–19,924 Hz, and the tuning range of the measurement result is 110,620.36 ppm. The sensitivities of the x-, y-, and z-axes of the magnetometer with driving current of 2 mA are 218.3, 74.33, and 7.5 μV/μT for ambient pressure of 760 torr. The resolutions of the x-, y-, and z-axes of the magnetometer with driving current of 2 mA are 3.302, 9.69, and 96 nT/√Hz for ambient pressure of 760 torr.
Bioreactor Co-Cultivation of High Lipid and Carotenoid Producing Yeast Rhodotorula kratochvilovae and Several Microalgae under Stress
Authors: Szotkowski Martin , Holub Jiří , Šimanský Samuel , Hubačová Klára , Sikorová Pavlína , Mariničová Veronika , Němcová Andrea , Márová Ivana
Published: May 2021

The co-cultivation of red yeasts and microalgae works with the idea of the natural transport of gases. The microalgae produce oxygen, which stimulates yeast growth, while CO2 produced by yeast is beneficial for algae growth. Both microorganisms can then produce lipids. The present pilot study aimed to evaluate the ability of selected microalgae and carotenogenic yeast strains to grow and metabolize in co-culture. The effect of media composition on growth and metabolic activity of red yeast strains was assessed simultaneously with microalgae mixotrophy. Cultivation was transferred from small-scale co-cultivation in Erlenmeyer flasks to aerated bottles with different inoculation ratios and, finally, to a 3L bioreactor. Among red yeasts, the strain R. kratochvilovae CCY 20-2-26 was selected because of the highest biomass production on BBM medium. Glycerol is a more suitable carbon source in the BBM medium and urea was proposed as a compromise. From the tested microalgae, Desmodesmus sp. were found as the most suitable for co-cultivations with R. kratochvilovae. In all co-cultures, linear biomass growth was found (144 h), and the yield was in the range of 8.78–11.12 g/L of dry biomass. Lipids increased to a final value of 29.62–31.61%. The FA profile was quite stable with the UFA portion at about 80%. Around 1.98–2.49 mg/g CDW of carotenoids with torularhodine as the major pigment were produced, ubiquinone production reached 5.41–6.09 mg/g, and ergosterol yield was 6.69 mg/g. Chlorophyll production was very low at 2.11 mg/g. Pilot experiments have confirmed that carotenogenic yeasts and microalgae are capable of symbiotic co-existence with a positive impact om biomass growth and lipid metabolites yields.
Cell Wall Proteome Profiling of a Candida albicans Fluconazole-Resistant Strain from a Lebanese Hospital Patient Using Tandem Mass Spectrometry—A Pilot Study
Authors: Awad Andy , El Khoury Pamela , Geukgeuzian Geovanni , Khalaf Roy A.
Published: May 2021

Candida albicans is an opportunistic pathogenic fungus responsible for high mortality rates in immunocompromised individuals. Azole drugs such as fluconazole are the first line of therapy in fungal infection treatment. However, resistance to azole treatment is on the rise. Here, we employ a tandem mass spectrometry approach coupled with a bioinformatics approach to identify cell wall proteins present in a fluconazole-resistant hospital isolate upon drug exposure. The isolate was previously shown to have an increase in cell membrane ergosterol and cell wall chitin, alongside an increase in adhesion, but slightly attenuated in virulence. We identified 50 cell wall proteins involved in ergosterol biosynthesis such as Erg11, and Erg6, efflux pumps such as Mdr1 and Cdr1, adhesion proteins such as Als1, and Pga60, chitin deposition such as Cht4, and Crh11, and virulence related genes including Sap5 and Lip9. Candidial proteins identified in this study go a long way in explaining the observed phenotypes. Our pilot study opens the way for a future large-scale analysis to identify novel proteins involved in drug-resistance mechanisms.
Contribution of HIV/AIDS-Related Human and Social Sciences Research to a Better Understanding of the Challenges of Hepatitis B Prevention, Diagnosis and Care
Authors: Bauquier Charlotte , Préau Marie
Published: May 2021

Recent scientific advances in hepatitis B virus research hint at the possibility of finding a cure in the medium term. In this context, the characterization of infected persons constitutes a major public health issue in terms of implementing adapted screening and prevention strategies. Overcoming the current challenges national health systems face in hepatitis B diagnosis is essential if the World Health Organization’s target of treating 80% of infected patients by 2030 is to be reached. These challenges reflect those previously faced in the fight against HIV/AIDS. Using the knowledge produced to date in Human and Social Sciences research in the fight against HIV/AIDS, we propose avenues of reflection to support and guide the development of research in the diagnosis of hepatitis B infection. More specifically, we present theoretical, methodological and epistemological considerations for how HSS research can be optimized in the following three HBV diagnosis-related areas: (i) access to screening; (ii) retention in care; and (iii) the integration of quality of life measurement in clinical trials.
Diversity and Oil Degradation Potential of Culturable Microbes Isolated from Chronically Contaminated Soils in Trinidad
Authors: Ramdass Amanda C. , Rampersad Sephra N.
Published: May 2021

Trinidad and Tobago is the largest producer of oil and natural gas in Central America and the Caribbean. Natural crude oil seeps, in addition to leaking petroleum pipelines, have resulted in chronic contamination of the surrounding terrestrial environments since the time of petroleum discovery, production, and refinement in Trinidad. In this study, we isolated microbes from soils chronically contaminated with crude oil using a culture-dependent approach with enrichment. The sampling of eight such sites located in the southern peninsula of Trinidad revealed a diverse microbial composition and novel oil-degrading filamentous fungi and yeast as single-isolate degraders and naturally occurring consortia, with specific bacterial species not previously reported in the literature. Multiple sequence comparisons and phylogenetic analyses confirmed the identity of the top degraders. The filamentous fungal community based on culturable species was dominated by Ascomycota, and the recovered yeast isolates were affiliated with Basidiomycota (65.23%) and Ascomycota (34.78%) phyla. Enhanced biodegradation of petroleum hydrocarbons is maintained by biocatalysts such as lipases. Five out of seven species demonstrated extracellular lipase activity in vitro. Our findings could provide new insights into microbial resources from chronically contaminated terrestrial environments, and this information will be beneficial to the bioremediation of petroleum contamination and other industrial applications.
Scanning Electron Microscope: A New Potential Tool to Replace Gram Staining for Microbe Identification in Blood Cultures
Authors: Haddad Gabriel , Bellali Sara , Takakura Tatsuki , Fontanini Anthony , Ominami Yusuke , Bou Khalil Jacques , Raoult Didier
Published: May 2021

Blood culture is currently the most commonly used method for diagnosing sepsis and bloodstream infections. However, the long turn-around-time to achieve microbe identification remains a major concern for clinical microbiology laboratories. Gram staining for preliminary identification remains the gold standard. We developed a new rapid strategy using a tabletop scanning electron microscope (SEM) and compared its performance with Gram staining for the detection of micro-organisms and preliminary identification directly from blood cultures. We first optimised the sample preparation for twelve samples simultaneously, saving time on imaging. In this work, SEM proved its ability to identify bacteria and yeasts in morphotypes up to the genus level in some cases. We blindly tested 1075 blood cultures and compared our results to the Gram staining preliminary identification, with MALDI-TOF/MS as a reference. This method presents major advantages such as a fast microbe identification, within an hour of the blood culture being detected positive, low preparation costs, and data traceability. This SEM identification strategy can be developed into an automated assay from the sample preparation, micrograph acquisition, and identification process. This strategy could revolutionise urgent microbiological diagnosis of infectious diseases.
The Effects of Chemical and Mechanical Stresses on Bacillus cereus and Pseudomonas fluorescens Single- and Dual-Species Biofilm Removal
Authors: Gomes Inês B. , Lemos Madalena , Fernandes Susana , Borges Anabela , Simões Lúcia C. , Simões Manuel
Published: May 2021

Biofilm control is mainly based on chemical disinfection, without a clear understanding of the role of the biocides and process conditions on biofilm removal. This study aims to understand the effects of a biocide (benzyldimethyldodecyl ammonium chloride—BDMDAC) and mechanical treatment (an increase of shear stress -τw) on single- and dual-species biofilms formed by Bacillus cereus and Pseudomonas fluorescens on high-density polyethene (HDPE). BDMDAC effects were initially assessed on bacterial physicochemical properties and initial adhesion ability. Then, mature biofilms were formed on a rotating cylinder reactor (RCR) for 7 days to assess the effects of chemical and mechanical treatments, and the combination of both on biofilm removal. The results demonstrated that the initial adhesion does not predict the formation of mature biofilms. It was observed that the dual-species biofilms were the most susceptible to BDMDAC exposure. The exposure to increasing τw emphasised the mechanical stability of biofilms, as lower values of τw (1.66 Pa) caused high biofilm erosion and higher τw values (17.7 Pa) seem to compress the remaining biofilm. In general, the combination of BDMDAC and the mechanical treatment was synergic in increasing biofilm removal. However, these were insufficient to cause total biofilm removal (100%; an average standard deviation of 11% for the method accuracy should be considered) from HDPE.
Effect of Coxsackievirus B4 Infection on the Thymus: Elucidating Its Role in the Pathogenesis of Type 1 Diabetes
Authors: Alhazmi Abdulaziz , Nekoua Magloire Pandoua , Michaux Hélène , Sane Famara , Halouani Aymen , Engelmann Ilka , Alidjinou Enagnon Kazali , Martens Henri , Jaidane Hela , Geenen Vincent , Hober Didier
Published: May 2021

The thymus gland is a primary lymphoid organ for T-cell development. Various viral infections can result in disturbance of thymic functions. Medullary thymic epithelial cells (mTECs) are important for the negative selection of self-reactive T-cells to ensure central tolerance. Insulin-like growth factor 2 (IGF2) is the dominant self-peptide of the insulin family expressed in mTECs and plays a crucial role in the intra-thymic programing of central tolerance to insulin-secreting islet β-cells. Coxsackievirus B4 (CVB4) can infect and persist in the thymus of humans and mice, thus hampering the T-cell maturation and differentiation process. The modulation of IGF2 expression and protein synthesis during a CVB4 infection has been observed in vitro and in vivo in mouse models. The effect of CVB4 infections on human and mouse fetal thymus has been studied in vitro. Moreover, following the inoculation of CVB4 in pregnant mice, the thymic function in the fetus and offspring was disturbed. A defect in the intra-thymic expression of self-peptides by mTECs may be triggered by CVB4. The effects of viral infections, especially CVB4 infection, on thymic cells and functions and their possible role in the pathogenesis of type 1 diabetes (T1D) are presented.
A Review of the Commercial Uses of Sulphate Minerals from the Titanium Dioxide Pigment Industry: The Case of Huelva (Spain)
Authors: Gázquez Manuel Jesús , Contreras Manuel , Pérez-Moreno Silvia María , Guerrero Jose Luis , Casas-Ruiz Melquiades , Bolívar Juan Pedro
Published: May 2021

This study was focused on the historical evolution of the waste management policy carried out by the Spanish industry devoted to titanium dioxide pigments manufacturing for minimising its environmental impact. This challenge was achieved by modifying the original chemical process and converting the originally dissolved sulphate and sulphuric acid present in the final streams of the factory into sulphate minerals (melanterite FeSO4·7H2O, szomolnokite FeSO4·H2O, and gypsum CaSO4·2H2O). These by-products were physicochemically, mineralogically and radiologically characterised in order to gain basic information for its subsequent commercial use. Some of the uses summarised in this study for both ferrous sulphates are as a supplier of iron to prevent chlorosis, animal food, manufacture of cement (to reduce Cr VI), primary flocculants for ferrous sulphates, magnetite nanoparticle and nano-Fe2O3 formation, production of magnetite concentrate, remediation of polluted soils with metals, and treatment of wastewaters. Red gypsum was analysed as a substitute for natural gypsum in the manufacture of cement, construction materials, inhibitor in soil erosion, and the immobilisation of heavy metals in agricultural soils and carbonation processes.
Geological Mapping Using Drone-Based Photogrammetry: An Application for Exploration of Vein-Type Cu Mineralization
Authors: Honarmand Mehdi , Shahriari Hadi
Published: May 2021

In this research, drone-based photogrammetry was utilized for mapping geology with the objective of mineral exploration in the Shahzadeh Abbas Cu deposit, Kerman province, Iran. Cu mineralization is of vein-type and follows geological structures. A low-cost drone was used to collect geological data. A spatial resolution of 3.26 cm was achieved by considering a flight altitude of 70 m. To reach the accuracy of less than 5 cm, 70% lateral and 80% front image overlaps were applied and 220 temporary ground control points (TGCPs) were used in an area of 2.02 km2. TGCPs were accurately positioned using DGPS-RTK measurements. Agisoft PhotoScan software was used for photogrammetric processing. The orthophoto product was performed for outlining geological units through visual interpretation. The digital elevation model (DEM) was converted to a hill-shade model in ArcGIS software to extract the geological structures such as faults and dikes. A draft geology map was prepared using orthophoto and hill-shade images to minimize the time and cost of the subsequent field work. Rock sampling was carried out and Cu-bearing veins were specified through field investigations. The geology map was finalized based on field work data and petrology studies. The geological survey indicated that diabase dikes with a northwest–southeast strike often host Cu mineralization in the study area. The position of Cu-bearing dikes was delineated for the next stage of the exploration program. This research demonstrated the time- and cost-effectiveness of using drone-based photogrammetry for preparing base geology maps for the exploration of vein-type mineralization in far districts with rough topography.
Milk Proteins—Their Biological Activities and Use in Cosmetics and Dermatology
Authors: Kazimierska Kinga , Kalinowska-Lis Urszula
Published: May 2021

Milk and colostrum have high biological potential, and due to their natural origin and non-toxicity, they have many uses in cosmetics and dermatology. Research is ongoing on their potential application in other fields of medicine, but there are still few results; most of the published ones are included in this review. These natural products are especially rich in proteins, such as casein, β-lactoglobulin, α-lactalbumin, lactoferrin, immunoglobulins, lactoperoxidase, lysozyme, and growth factors, and possess various antibacterial, antifungal, antiviral, anticancer, antioxidant, immunomodulatory properties, etc. This review describes the physico-chemical properties of milk and colostrum proteins and the natural functions they perform in the body and compares their composition between animal species (cows, goats, and sheep). The milk- and colostrum-based products can be used in dietary supplementation and for performing immunomodulatory functions; they can enhance the effects of certain drugs and can have a lethal effect on pathogenic microorganisms. Milk products are widely used in the treatment of dermatological diseases for promoting the healing of chronic wounds, hastening tissue regeneration, and the treatment of acne vulgaris or plaque psoriasis. They are also increasingly regarded as active ingredients that can improve the condition of the skin by reducing the number of acne lesions and blackheads, regulating sebum secretion, ameliorating inflammatory changes as well as bestowing a range of moisturizing, protective, toning, smoothing, anti-irritation, whitening, soothing, and antiaging effects.
Antifungal Activity of Chemical Constituents from Piper pesaresanum C. DC. and Derivatives against Phytopathogen Fungi of Cocoa
Authors: Chitiva-Chitiva Luis C. , Ladino-Vargas Cristóbal , Cuca-Suárez Luis E. , Prieto-Rodríguez Juliet A. , Patiño-Ladino Oscar J.
Published: May 2021

In this study, the antifungal potential of chemical constituents from Piper pesaresanum and some synthesized derivatives was determined against three phytopathogenic fungi associated with the cocoa crop. The methodology included the phytochemical study on the aerial part of P. pesaresanum, the synthesis of some derivatives and the evaluation of the antifungal activity against the fungi Moniliophthora roreri, Fusarium solani and Phytophthora sp. The chemical study allowed the isolation of three benzoic acid derivatives (1–3), one dihydrochalcone (4) and a mixture of sterols (5–7). Seven derivatives (8–14) were synthesized from the main constituents, of which compounds 9, 10, 12 and 14 are reported for the first time. Benzoic acid derivatives showed strong antifungal activity against M. roreri, of which 11 (3.0 ± 0.8 µM) was the most active compound with an IC50 lower compared with positive control Mancozeb® (4.9 ± 0.4 µM). Dihydrochalcones and acid derivatives were active against F. solani and Phytophthora sp., of which 3 (32.5 ± 3.3 µM) and 4 (26.7 ± 5.3 µM) were the most active compounds, respectively. The preliminary structure–activity relationship allowed us to establish that prenylated chains and the carboxyl group are important in the antifungal activity of benzoic acid derivatives. Likewise, a positive influence of the carbonyl group on the antifungal activity for dihydrochalcones was deduced.
Essential Oils from Residual Foliage of Forest Tree and Shrub Species: Yield and Antioxidant Capacity
Authors: Mediavilla Irene , Guillamón Eva , Ruiz Alex , Esteban Luis Saúl
Published: May 2021

Increasing applications and markets for essential oils could bring new opportunities for cost-effective and sustainable management of unused forestry biomass; however, better knowledge of the production and application of such essential oils is necessary. The objective of this work is to contribute to greater knowledge of the essential oil production on a pilot scale from foliage biomass of wild shrubs and tree residues produced in some forestry enhancement operations and to study their antioxidant capacity (ORAC—oxygen radical absorbance capacity). Fresh biomass (twigs) of seven species (E. globulus, E. nitens, P. pinaster, P. sylvestris, R. officinalis, C. ladanifer, and J. communis) was manually collected in Spain in two different periods and was ground at 30 mm and distilled in a 30 L stainless steel still with saturated steam. The essential oil components were identified by GC–MS and quantified by GC–FID, and their antioxidant activity was determined with the ORAC method. Promising results on essential oil yield were obtained with E. globulus, E. nitens, R. officinalis, and J. communis. All essential oils studied exhibited antioxidant capacity by the ORAC assay, particularly that from C. ladanifer. Moreover, oxygenated sesquiterpenes contents, one of the minor components of oils, were significantly correlated with ORAC values.
Polydopamine Nanocluster Embedded Nanofibrous Membrane via Blow Spinning for Separation of Oil/Water Emulsions
Authors: Liu Zhenglian , Xu Ziling , Liu Chaoqi , Zhao Yajing , Xia Qingyin , Fang Minghao , Min Xin , Huang Zhaohui , Liu Yan’gai , Wu Xiaowen
Published: May 2021

Developing a porous separation membrane that can efficiently separate oil–water emulsions still represents a challenge. In this study, nanofiber membranes with polydopamine clusters polymerized and embedded on the surface were successfully constructed using a solution blow-spinning process. The hierarchical surface structure enhanced the selective wettability, superhydrophilicity in air (≈0°), and underwater oleophobicity (≈160.2°) of the membrane. This membrane can effectively separate oil–water emulsions, achieving an excellent permeation flux (1552 Lm−2 h−1) and high separation efficiency (~99.86%) while operating only under the force of gravity. When the external driving pressure was increased to 20 kPa, the separation efficiency hardly changed (99.81%). However, the permeation flux significantly increased to 5894 Lm−2 h−1. These results show that the as-prepared polydopamine nanocluster-embedded nanofiber membrane has an excellent potential for oily wastewater treatment applications.
Special Issue: NMR-Based Metabolomics
Authors: Pérez-Trujillo Miriam , Athersuch Toby J.
Published: May 2021

Nuclear magnetic resonance (NMR) spectroscopy remains one of the core analytical platforms for metabolomics, providing complementary chemical information to others, such as mass spectrometry, and offering particular advantages in some areas of research on account of its inherent robustness, reproducibility, and phenomenal dynamic range [...]