bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits
Authors: Dabbou Sihem , Ferrocino Ilario , Gasco Laura , Schiavone Achille , Trocino Angela , Xiccato Gerolamo , Barroeta Ana C. , Maione Sandra , Soglia Dominga , Biasato Ilaria , Cocolin Luca , Gai Francesco , Nucera Daniele Michele
Published: July 2020

This study aimed to evaluate the in vitro antimicrobial activities of two types of insect fats extracted from black soldier fly larvae (HI, Hermetia illucens L.) and yellow mealworm larvae (TM, Tenebrio molitor L.) and their effects as dietary replacement of soybean oil (S) on cecal fermentation pattern, and fecal and cecal microbiota in rabbits. A total of 120 weaned rabbits were randomly allotted to three dietary treatments (40 rabbits/group) —a control diet (C diet) containing 1.5% of S and two experimental diets (HI diet (HID) and TM diet (TMD)), where S was totally substituted by HI or TM fats during the whole trial that lasted 41 days. Regarding the in vitro antimicrobial activities, HI and TM fats did not show any effects on Salmonella growth. Yersinia enterocolitica showed significantly lower growth when challenged with HI fats than the controls. The insect fat supplementation in rabbit diets increased the contents of the cecal volatile fatty acids when compared to the control group. A metataxonomic approach was adopted to investigate the shift in the microbial composition as a function of the dietary insect fat supplementation. The microbiota did not show a clear separation as a function of the inclusion, even if a specific microbial signature was observed. Indeed, HI and TM fat supplementation enriched the presence of Akkermansia that was found to be correlated with NH3-N concentration. An increase in Ruminococcus, which can improve the immune response of the host, was also observed. This study confirms the potential of HI and TM fats as antibacterial feed ingredients with a positive influence on the rabbit cecal microbiota, thus supporting the possibility of including HI and TM fats in rabbit diets.
Relationship between the Quality of Colostrum and the Formation of Microflora in the Digestive Tract of Calves
Authors: Puppel Kamila , Gołębiewski Marcin , Konopka Katarzyna , Kunowska-Slósarz Małgorzata , Slósarz Jan , Grodkowski Grzegorz , Przysucha Tomasz , Balcerak Marek , Madras-Majewska Beata , Sakowski Tomasz
Published: July 2020

The aim of the study was to determine the relationship between the quality of colostrum and the formation of intestinal bacterial microflora in calves from birth to day 7. Seventy-five multiparous Polish Holstein–Friesian cows were selected. Colostrum samples were collected individually up to two hours after calving. The analysis was carried out on 75 calves; which were divided into three groups based on the colostrum quality class of the first milking. Faecal samples were collected rectally from each calf on its seventh day of life. Calves were weighed twice; on days 0 and 7 of life. It has been shown that with a higher concentration of colostrum protein fraction, primarily immunoglobulins, the colonisation of anaerobic bacteria occurs faster. Colostrum with a density >1.070 g/cm3 promoted the significant development of Lactobacilli and Bifidobacterium spp. which at the same time contributed to the reduction of unfavourable microflora, such as Coliforms or Enterococci. Regardless of the initial body weight, daily weight gains were highest for calves fed with colostrum with a density >1.070 g/cm3. The study showed a significant influence of colostrum quality class on the formation of the intestinal microflora and the daily weight gains of calves.
Escherichia coli Producing Extended-Spectrum β-lactamases (ESBL) from Domestic Camels in the Canary Islands: A One Health Approach
Authors: Carvalho Isabel , Tejedor-Junco María Teresa , González-Martín Margarita , Corbera Juan Alberto , Silva Vanessa , Igrejas Gilberto , Torres Carmen , Poeta Patrícia
Published: July 2020

Objective: This work aimed to determine the carriage rate of ESBL-producing Escherichia coli as well as their genetic characteristics in camels from the Canary Islands, Spain. Methods: Fecal samples were recovered from 58 healthy camels from Gran Canaria (n = 32) and Fuerteventura Islands (n = 26) during July 2019. They were seeded on MacConkey (MC) agar no supplemented and supplemented (MC + CTX) with cefotaxime (2 µg/mL). Antimicrobial susceptibility was determined by disk diffusion test (CLSI, 2018). The presence of blaCTX-M, blaSHV, blaTEM,blaCMY-2 and blaOXA-1/48 genes was tested by PCR/sequencing. Furthermore, the mcr-1 (colistin resistance), tetA/tetB (tetracycline resistance), int1 (integrase of class 1 integrons) and stx1,2 genes were analyzed. Phylogenetic groups and sequence types were determined by specific-PCR/sequencing for selected isolates. Results: E. coli was obtained from all the 58 camels in MC media (100%) and in five of them in MC + CTX media (8.6%). Furthermore, 63.8% of E. coli isolates recovered from MC agar were susceptible to all the antibiotics tested. The five E. coli isolates recovered from MC + CTX media were characterized and two of them were ESBL-producers (3.4%). Both ESBL-producer isolates carried the blaCTX-M-15 gene and belonged to the lineages ST3018 (phylogroup A) and ST69 (phylogroup B1). The 3 ESBL-negative isolates recovered from MC-CTX plates were ascribed to phylogroup-B1. Conclusions: Camels can be a source of ESBL-producer bacteria, containing the widespread blaCTX-M-15 gene associated with the lineages ST3018 and ST69.
Nutrient Digestibility, Growth Performance, and Blood Indices of Boschveld Chickens Fed Seaweed-Containing Diets
Authors: Nhlane Lindani Trevor , Mnisi Caven Mguvane , Mlambo Victor , Madibana Molatelo Junior
Published: July 2020

Seaweeds possess a wide range of bioactive compounds that can be beneficial in sustainable intensification systems. This study explored the effect of green seaweed (Ulva spp.) meal (SWM) on apparent nutrient digestibility and physiological responses of Boschveld indigenous chickens. Two-hundred and seventy-five hens (202.4 ± 6.65 g live-weight; 4-weeks-old) were reared on five isoenergetic and isoproteic diets formulated by adding SWM at 0 (SW0), 20 (SW20), 25 (SW25), 30 (SW30), and 35 g/kg (SW35). Neutral detergent fibre digestibility quadratically responded (R2 = 0.244; p = 0.042) to SWM levels. No significant dietary influences were observed on apparent nutrient digestibility values. Repeated measures analysis showed significant diet × week interaction effect on weekly feed intake and growth performance. Dietary inclusion of SWM increased (p < 0.05) feed intake and overall body weight gain but not feed conversion efficiency. No significant linear and quadratic trends were observed for blood parameters except for basophils (R2 = 0.172; p = 0.047), which linearly declined with SWM levels. Dietary SWM inclusion only influenced (p < 0.05) mean corpuscular volume, mean corpuscular concentration, and mean corpuscular haemoglobin concentration. We concluded that seaweeds have the potential to be used as a feed ingredient for indigenous chickens.
Septic Tenosynovitis of the Digital Flexor Tendon Sheath in 83 Cattle
Authors: Hund Alexandra , Senn Markus , Kofler Johann
Published: July 2020

Septic tenosynovitis of the digital flexor tendon sheath (DFTS) is the second most prevalent infection of deeper structures of the distal limb in cattle, after septic arthritis of the distal interphalangeal (DIP) joint. Depending on the type of infection and the involvement of adjacent anatomical structures, various surgical techniques may be used for therapy: Incising the DFTS to resect one or both digital flexor tendons (RDFT), additional resection of the DIP joint (RDIP) or additional digital amputation (RAMP). Our goal was to describe clinical findings and outcome in cattle patients (euthanasia vs. treatment) and the success of surgical methods including improvement of locomotion and postoperative survival time (POST). Data of eighty-three cattle with a mean age of 4.3 years were reviewed in this retrospective study. Overall, 57.7% of tenosynovitis cases were in the lateral DFTS of a hind limb. Fifty-five cattle were treated surgically; the remaining 28 cattle were euthanized following diagnosis. The median cumulative POST was 17.3, 83.1, and 11.9 months for RDFT, RDIP, and RAMP, respectively. Fatal postoperative complications occurred in three cattle. We conclude that the applied methods were successful and allowed the animals to almost reach the average life expectancy of an Austrian dairy cow.
Moving in the Dark—Evidence for an Influence of Artificial Light at Night on the Movement Behaviour of European Hedgehogs (Erinaceus europaeus)
Authors: Berger Anne , Lozano Briseida , Barthel Leon M. F. , Schubert Nadine
Published: July 2020

With urban areas growing worldwide comes an increase in artificial light at night (ALAN), causing a significant impact on wildlife behaviour and its ecological relationships. The effects of ALAN on nocturnal and protected European hedgehogs (Erinaceus europaeus) are unknown but their identification is important for sustainable species conservation and management. In a pilot study, we investigated the influence of ALAN on the natural movement behaviour of 22 hedgehogs (nine females, 13 males) in urban environments. Over the course of four years, we equipped hedgehogs at three different study locations in Berlin with biologgers to record their behaviour for several weeks. We used Global Positioning System (GPS) tags to monitor their spatial behaviour, very high-frequency (VHF) loggers to locate their nests during daytime, and accelerometers to distinguish between active and passive behaviours. We compared the mean light intensity of the locations recorded when the hedgehogs were active with the mean light intensity of simulated locations randomly distributed in the individual’s home range. We were able to show that the ALAN intensity of the hedgehogs’ habitations was significantly lower compared to the simulated values, regardless of the animal’s sex. This ALAN-related avoidance in the movement behaviour can be used for applied hedgehog conservation.
Short Chain Fatty Acids Modulate the Growth and Virulence of Pathosymbiont Escherichia coli and Host Response
Authors: Zhang Shiying , Dogan Belgin , Guo Cindy , Herlekar Deepali , Stewart Katrina , Scherl Ellen J. , Simpson Kenneth W.
Published: July 2020

Short chain fatty acids (SCFA), principally acetate, propionate, and butyrate, are produced by fermentation of dietary fibers by the gut microbiota. SCFA regulate the growth and virulence of enteric pathogens, such as enterohemorrhagic E. coli (EHEC), Klebsiella and Salmonella. We sought to investigate the impact of SCFA on growth and virulence of pathosymbiont E. coli associated with inflammatory bowel disease (IBD) and colorectal cancer (CRC), and their role in regulating host responses to bacterial infection in vitro. We found that under ileal conditions (pH = 7.4; 12 mM total SCFA), SCFA significantly (p < 0.05) potentiate the growth and motility of pathosymbiont E. coli. However, under colonic conditions (pH = 6.5; 65 to 123 mM total SCFA), SCFA significantly (p < 0.05) inhibit growth in a pH dependent fashion (up to 60%), and down-regulate virulence gene expression (e.g., fliC, fimH, htrA, chuA, pks). Functional analysis reveals that colonic SCFA significantly (p < 0.05) inhibit E. coli motility (up to 95%), infectivity (up to 60%), and type 1 fimbria-mediated agglutination (up to 50%). In addition, SCFA significantly (p < 0.05) inhibit the activation of NF-κB, and IL-8 production by epithelial cells. Our findings provide novel insights on the role of the regional chemical microenvironment in regulating the growth and virulence of pathosymbiont E. coli and opportunities for therapeutic intervention.
Synthesis and Characterization of the Properties of Ceria Nanoparticles with Tunable Particle Size for the Decomposition of Chlorinated Pesticides
Authors: Skrbek Kryštof , Bartůněk Vilém , Lojka Michal , Sedmidubský David , Jankovský Ondřej
Published: July 2020

Ceria nanoparticles are well known for their catalytic properties, which are commonly used in the automotive industry and other chemical branches. In this contribution, ceria nanoparticles were prepared by a simple precipitation reaction of cerium (IV) sulfate and ammonia. Such nanoparticles were very fine, with an approximate crystallite size of 2 nm. Crystallite sizes of these nanoparticles were adjusted by controlled heating, showing different catalytic properties. After thermal treatment, the surface area and crystallite size of nanoparticles were also compared by means of XRD and a sorption analyzer utilizing Brunauer–Emmet–Teller (BET). Morphology was studied by SEM, high-resolution transmission electron microscopy (HR-TEM) and selected area electron diffraction (SAED). Thin layers of ceria nanoparticles on silica glass and alumina ceramic underlays were also prepared and studied by SEM and EDS to demonstrate the possibility of ceria immobilization using thermal treatment of raw nanoparticles. The catalytic activity of the nanoparticles was tested on a 2,4,6-trichlorophenol aqueous solution and compared by UV–VIS spectroscopy.
Assessment of the Length and Depth of Delamination-Type Defects Using Ultrasonic Guided Waves
Authors: Samaitis Vykintas , Mažeika Liudas , Rekuvienė Regina
Published: July 2020

Fiber-reinforced composite laminates are being increasingly used in various engineering components in the sectors of aerospace and green energy. Due to impacts throughout the service life of the structure, matrix breakage and delaminations significantly altering the structural integrity of the laminate can occur. Hence, robust guided wave structural health monitoring systems are required to ensure continuous safety of engineering structures. In this paper, the ultrasonic method based on the analysis of A0 mode reflecting within the defected area has been proposed to extract the length and the depth of the delamination-type defect. The technique proposed in this study extracts the depth of the damage by analyzing the magnitude variations of direct A0 mode which are caused by the difference of wave velocities in the upper and lower sub-laminates. This results in an altering and frequency-dependent forward-scattered amplitude of direct A0 mode. Furthermore, the proposed approach uses previously obtained information about the depth of the defect, which allows for the determination of the phase velocities of A0 and S0 modes in the upper and lower sub-laminates. As a result, the accuracy of the damage length estimation is increased. The performance of the proposed method was proven with 2D and 3D numerical simulations and experiments on samples with artificial defects. The method validation results showed that the proposed method with some limitations is capable of extracting the length of delamination with an approximate error below 6%.
Modulation and Control Technology for Generating Movable Super-Diffraction Optical Needle by Oblique Incidence
Authors: Wang Simo , Yu Siyang , Li Fanxing , Peng Fuping , Du Jialin , Qi Bo , Shi Lifang , Yan Wei
Published: July 2020

The movable super-diffraction optical needle (MSON) is a tightly focused beam like a “needle”, which can realize vector scanning on the focusing plane. Not only does it have a long focal depth, but its resolution also exceeds the diffraction limit. The modulation and control technology required for generating MSON by oblique incidence is explored in this manuscript for the purpose of processing high-aspect-ratio, sub-wavelength structures. As the optical needle generated by traditional methods is static and sensitive to variation of the angle information of the incident beam, here we introduce a confocal scanning system by using a two-dimensional galvanometer system, a scan lens, and a tube lens to control the oblique incidence angle. The effects of the oblique incidence angle on the resolution, depth of focus, uniformity, and side lobes of the MSON were analyzed. Further, the voltage-controlled liquid crystal located between the scan lens and the 2D galvanometer system can be used to compensate for the additional phase difference caused by oblique incidence. The aspect ratio is defined as the ratio of depth of focus to resolution. By modulating and controlling the light field, the MSON with high aspect ratio (7.36), sub-diffractive beam size (0.42λ), and long depth of focus (3.09λ) has been obtained with homogeneous intensity, and suppressed side lobes. High speed, high axial positioning tolerance, and high-resolution laser processing can also be achieved, which removes the restrictions presented by traditional laser processing technology, for which high resolution and long depth of focus cannot be achieved simultaneously.
Monotonic and Fatigue Properties of Steel Material Manufactured by Wire Arc Additive Manufacturing
Authors: Wächter Michael , Leicher Marcel , Hupka Moritz , Leistner Chris , Masendorf Lukas , Treutler Kai , Kamper Swenja , Esderts Alfons , Wesling Volker , Hartmann Stefan
Published: July 2020

In this study, the monotonic and cyclic material properties of steel material of medium static strength produced additively in the wire arc additive manufacturing (WAAM) process were investigated. This investigated material is expected to be particularly applicable to the field of mechanical engineering, for which practical applications of the WAAM process are still pending and for which hardly any characteristic values can be found in the literature so far. The focus of the investigation was, on the one hand, to determine how the material characteristics are influenced by the load direction in relation to the layered structure and, on the other hand, how they are affected by different interlayer temperatures. For this purpose, monotonic tensile tests were carried out at room temperature as well as at elevated temperatures, and the cyclic material properties were determined. In addition, the hardness of the material and the residual stresses induced during production were measured and compared. In addition to the provision of characteristic properties for the investigated material, it was aimed to determine the extent to which the interlayer temperature influences the strength characteristics, since this can have a considerable influence on the production times and, thus, the economic efficiency of the process.
A Workspace Visualization Method for a Multijoint Industrial Robot Based on the 3D-Printing Layering Concept
Authors: Fu Guoqiang , Tao Chun , Gu Tengda , Lu Caijiang , Gao Hongli , Deng Xiaolei
Published: July 2020

The workspace of a robot provides the necessary constraint information for path planning and reliable control of the robot. In this paper, a workspace visualization method for a multijoint industrial robot is proposed to obtain a detailed workspace by introducing the 3D-printing layering concept. Firstly, all possible joint-angle groups of one pose in the joints’ ranges are calculated in detail according to the POE (product of exponential) theory-based forward-kinematics expressions of the multijoint industrial robot. Secondly, a multisolution selection method based on the key degree of the joint is proposed to select the appropriate joint-angle groups. The key degrees of all joints and their key order are obtained according to the sensitivity expressions of all joint angles, calculated from the Jacobian matrix of the robot. One principle based on the smallest differences of the nominal angle is established to select the possible solutions for one joint from the possible solutions for the joint with the smaller key order. The possible solutions for the joint with the highest key order are the appropriate joint-angle group. Thirdly, a workspace visualization method based on the layering concept of 3D printing is presented to obtain a detailed workspace for a multijoint industrial robot. The boundary formula of each layer is derived by forward kinematics, which is expressed as a circle or a ring. The maximum and minimum values of the radius are obtained according to the travel range of the joint angles. The height limitations of all layers are obtained with forward kinematics. A workspace boundary-extraction method is presented to obtain the array of path points of the boundary at each layer. The proposed postprocessing method is used to generate the joint-angle code of each layer for direct 3D printing. Finally, the effectiveness of the multisolution selection method and the workspace visualization method were verified by simulation and experiment.
Effect of the Surface Morphology of TiO2 Nanotubes on Photocatalytic Efficacy Using Electron-Transfer-Based Assays and Antimicrobial Tests
Authors: Lia Frederick , Farrugia Clayton , Buccheri Maria Antonietta , Rappazzo Giancarlo , Zammit Edwin , Rizzo Alex , Grech Maurice , Refalo Paul , Abela Stephen
Published: July 2020

The application of titanium oxide nanotubes for the removal of contaminants from freshwater is a rapidly growing scientific interest, especially when it comes to water conservation strategies. In this study we employed four different titanium oxide nanotube surfaces, prepared by a two-electrode anodic oxidation. Two of the surfaces were synthesised in aqueous media, while the other two surfaces were synthesised in ethylene glycol. One of the arrays synthesised in the organic medium was impregnated with silver nanoparticles, while the remaining surfaces were not. The chemical reactivity of the various surfaces was assessed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as free electron sensitive probe molecules, in parallel with tannic acid degradation and copper ion reducing capacity. The potential antimicrobial activity of the surfaces was assessed against a panel of microorganisms composed of yeast, fungi, Gram-positive and Gram-negative bacteria. Field emission scanning electron microscopy revealed that surfaces produced in the aqueous medium had a smaller tube length and a smaller tube diameter. It was noted that one of the materials using sodium sulfate as the supporting electrolyte had the most irregular nanostructure morphology with tubes growing to the side rather than vertically. The structural variation of the surfaces directly reflected both the chemical and biological activity, with the nanotubes formed in ethylene glycol showing the fastest rates in the stabilization of DPPH and ABTS radicals, the fastest tannic acid decomposition under various pH conditions and the fastest metal reducing activity. Furthermore, the surface containing silver and its bare counterpart showed the most effective antimicrobial activity, removing approximately 82% of Gram-negative bacteria, 50% of Gram-positive bacteria, 70% of yeast and 40% of fungi, with Gram-negative bacteria being the most susceptible to these surfaces.
Numerical Study of a Polarization Selective Visual Optical Switch
Authors: Wen Kui , Zhang Zhaojian , Jiang Xinpeng , He Jie , Yang Junbo
Published: July 2020

The subwavelength structure based on metamaterial has been widely used in the application of structure color due to its unique characteristics. Here, a composite structure consisting of a circular hole and different number of metal strips is proposed. By changing the polarization direction of the incident light, the resonance peak in the visible light range shifts, and the material presents different colors. At the same time, take advantage of the shift of the resonance peak, a plasmonic optical switch, of which on/off states can be indicated by the structural color, is constructed, and the maximum contrast ratio is more than 20 dB. In addition, by means of changing the geometric parameters and materials of the metal strip, the colors presented basically cover the whole visible light range. This method theoretically proves the feasibility of using subwavelength structure to construct visual plasmonic optical switch using structural color in the visible light range, which provides a broad prospect for the application of multiple physical mechanism in nanostructure design.
Empirical Analysis for Impact of High-Speed Rail Construction on Interregional Dependency
Authors: Zheng Meina , Liu Feng , Guo Xiucheng , Li Juchen
Published: July 2020

The opening of the high-speed rail (HSR) resulted in significant changes in the transportation network of Korea. The new HSR construction was expected to become a new engine of local economic growth. However, there was a controversy regarding whether the connection between regions intensifies the concentration of socio-economic activities in the metropolis (straw effect) or contributes to the balance of regional development (sprawl effect). More increasing attention had been devoted to studying the “straw effects” caused by the newly built HSR networks on interregional social-economic activities. Despite considerable research on the benefit achieved from HSR construction, little has focused on the negative externalities resulting from it. This paper examined the potential “straw effects” of two new HSR lines through constructing the indicator of interregional dependency that measured one city’s level of dependency on another one. In order to exclude the interference of lurking variables, five metropolitan cities were selected as case studies. The empirical results, based on a panel data model, revealed that the larger the economic scale of the target city, the lower the level of dependency on other cities, and there existed a “straw effect” on HSR development in terms of Seoul.
Thermometry in a Multipole Ion Trap
Authors: Nötzold Markus , Hassan Saba Zia , Tauch Jonas , Endres Eric , Wester Roland , Weidemüller Matthias
Published: July 2020

We present a characterization of the ions’ translational energy distribution in a multipole ion trap. A linear mapping between the energy distribution of the trapped ions onto the ions’ time-of-flight (TOF) to a detector is demonstrated. For low ion temperatures, a deviation from linearity is observed and can be attributed to the emergence of multiple potential minima. The potential landscape of the trapped ions is modeled via the finite element method, also accounting for subtleties such as surface-charge accumulation. We demonstrate the validity of our thermometry method by simulating the energy distribution of the ion ensemble thermalized with buffer gas using a Molecular Dynamics (MD) simulation. A comparison between the energy distribution of trapped ions in different multipole trap configurations—i.e., with hyperbolic rods, cylindrical rods, and cylindrical wires—is provided. With these findings, one can map the temperature of the trapped ions down to the Kelvin regime using their TOF distributions. This enables future studies on sympathetic cooling and chemical reactions involving ions in multipole traps.
Reconfigurable Terahertz Metamaterial Using Split-Ring Meta-Atoms with Multifunctional Electromagnetic Characteristics
Authors: Liao Yuhang , Lin Yu-Sheng
Published: July 2020

We propose a reconfigurable terahertz (THz) metamaterial (RTM) to investigate its multifunctional electromagnetic characteristics by moving the meta-atoms of split-ring resonator (SRR) array. It shows the preferable and capable adjustability in the THz frequency range. The electromagnetic characteristics of the proposed RTM device are compared and analyzed by moving the meta-atoms in different polarized transverse magnetic (TM) and transverse electric (TE) modes. The symmetrical meta-atoms of RTM device exhibit a resonant tuning range of several tens of GHz and the asymmetrical meta-atoms of RTM device exhibit the better tunability. Therefore, an RTM device with reconfigurable meta-atoms possesses the resonance shifting, polarization switching, electromagnetically induced transparency (EIT) switching and multiband to single-band switching characteristics. This proposed RTM device provides the potential possibilities for the use of THz-wave optoelectronics with tunable resonance, EIT analog and tunable multiresonance characteristics.
Quantitative Investigation of Surface Charge Distribution and Point Probing Characteristics of Spherical Scattering Electrical Field Probe for Precision Measurement of Miniature Internal Structures with High Aspect Ratios
Authors: Bian Xingyuan , Cui Junning , Lu Yesheng , Zhao Yamin , Cheng Zhongyi , Tan Jiubin
Published: July 2020

For precision measurement of miniature internal structures with high aspect ratios, a spherical scattering electrical field probe (SSEP) is proposed based on charge signal detection. The characteristics and laws governing surface charge distribution on the probing ball of the SSEP are analyzed, with the spherical scattering electrical field modeled using a 3D seven-point finite difference method. The model is validated with finite element simulation by comparing with the analysis results of typical situations, in which probing balls of different diameters are used to probe a grounded plane with a probing gap of 0.3 μm. Results obtained with the proposed model and finite element method (FEM) simulation indicate that 31% of the total surface charge on a ϕ1 mm probing ball concentrates in an area that occupies 1% of the total probing ball surface. Moreover, this surface charge concentration remains unchanged when the surface being measured varies in geometry, or when the probing gap varies in sensing range. Based on this, the SSEP has realized approximate point probing capability with a virtual “needle” of electrical effect. Together with its non-contact sensing characteristics and 3D isotropy, it can, therefore, be concluded that the SSEP has great potential to be an ideal solution for precision measurement of miniature internal structures with high aspect ratios.
Predicting the Width of Corrosion-Induced Cracks in Reinforced Concrete Using a Damage Model Based on Fracture Mechanics
Authors: Okazaki Shinichiro , Okuma Chisato , Kurumatani Mao , Yoshida Hidenori , Matsushima Manabu
Published: July 2020

Using a finite-element scheme based on a damage model, a numerical system is developed to predict cracks in reinforced concrete beams due to corrosion expansion. The numerical results show that the width of such cracks is affected considerably by (i) the shape of the reinforcing bar, (ii) the presence of stirrups, and (iii) the number of main reinforcement bars. Specimens of reinforced concrete beams are fabricated to simulate those used in the analysis, and we determine how the crack width is related to the amount of the reinforcing bar corrosion through electrolytic corrosion experiments. The experimental results are used to assess the validity of the numerical ones, and the latter are considered to reproduce the former.
Macedonian Cultural Plurality at the Crossroads of the Balkans: Drama, Music and Dance
Authors: Zdravkova Djeparoska Sonja
Published: July 2020

Defining the Balkans as a geographic, cultural and semantic entity triggers an interpretation of them as some idea, concept, oftentimes even a stereotype. The Balkans are usually interpreted as a singular entity, generalized and set in a single framework. That generalized view is often ambivalent. The Balkans are often interpreted and presented as a ‘powder keg’, a ‘bridge between the East and the West’, a part of Europe that is simply different, a place of strong emotions and attractive forms of music and dance, etc. However, the Balkans represent a set of cultural units that are in constant interaction, with each of the cultures of the Balkans being specific and authentic. Macedonia, as one of the pages of the ‘Balkan story’, will be presented at three levels—regarding its drama, music and dance. The specific characteristics of the music and the dance fields will be presented through their most significant features and examples, and the treatment of the topic of the Balkans in Macedonian drama will be covered as well. The analysis confirms that generalization is impossible even within a single culture, as each artist and medium of performance has its own unique expression. The cultural forms of Macedonian culture are only part of the wider pluralistic representation of the Balkans. It may be offered under the Balkans as the common denominator, but the truth is that this/representation/concept is polyvalent, multicultural, polysemic and extremely rich.
The Impact of Stochastic Physics-Based Hybrid GSI/EnKF Data Assimilation on Hurricane Forecasts Using EMC Operational Hurricane Modeling System
Authors: Zhang Zhan , Tong Mingjing , Sippel Jason A. , Mehra Avichal , Zhang Banglin , Wu Keqin , Liu Bin , Dong Jili , Ma Zaizhong , Winterbottom Henry , Wang Weiguo , Zhu Lin , Liu Qingfu , Kim Hyun-Sook , Thomas Biju , Sheinin Dmitry , Bi Li , Tallapragada Vijay
Published: July 2020

The National Oceanic and Atmospheric Administration’s (NOAA) cloud-permitting high-resolution operational Hurricane Weather and Research Forecasting (HWRF) model includes the sophisticated hybrid grid-point statistical interpolation (GSI) and Ensemble Kalman Filter (EnKF) data assimilation (DA) system, which allows assimilating high-resolution aircraft observations in tropical cyclone (TC) inner core regions. In the operational HWRF DA system, the flow-dependent background error covariance matrix is calculated from the HWRF self-cycled 40-member ensemble. This DA system has proved to provide improved initial TC structure and therefore improved TC track and intensity forecasts. However, the uncertainties from the model physics are not taken into account in the FY2017 version of the HWRF DA system. In order to further improve the HWRF DA system, the stochastic physics perturbations are introduced in the HWRF DA, including the cumulus convection scheme, the planetary boundary layer (PBL) scheme, and model surface physics (drag coefficient), for HWRF-based ensembles. This study shows that both TC initial conditions and TC track and intensity forecast skills are improved by adding stochastic model physics in the HWRF self-cycled DA system. It was found that the improvements in the TC initial conditions and forecasts are the results of ensemble spread increases which realistically represent the model background error covariance matrix in HWRF DA. For all 2016 Atlantic storms, the TC track and intensity forecast skills are improved by about ~3% and 6%, respectively, compared to the control experiment. The case study shows that the stochastic physics in HWRF DA is especially helpful for those TCs that have inner-core high-resolution aircraft observations, such as tail Doppler radar (TDR) data.
Estimating Methane Emissions from a Dairy Farm Using a Computer Program
Authors: Nowakowicz-Dębek Bożena , Wlazło Łukasz , Szymula Agnieszka , Ossowski Mateusz , Kasela Martyna , Chmielowiec-Korzeniowska Anna , Bis-Wencel Hanna
Published: July 2020

The aim of our study was to estimate methane (CH4) emissions from a dairy cattle farm using a computer application. Emissions of CH4 in the air were forecast for a representative dairy farm raising Holstein-Friesian cows. The cowshed was equipped with a mechanical forced ventilation system with a centrally located ventilation duct. The volume of emissions from the emitter was established, taking into account meteorological conditions. For one year of operation of the emitter, the average annual emission was 1.301 kg/h. The maximum emission of CH4 was estimated at 3.51 kg/h. These data can be helpful in estimating the environmental burden of a dairy farm and in determining the role of ruminants in global warming.
Communication and Hazard Perception Lessons from Category Five Hurricane Michael
Authors: Senkbeil Jason , Myers Laura , Jasko Susan , Reed Jacob , Mueller Rebecca
Published: July 2020

Hurricane Michael made landfall on 10 October 2018 as only the third Saffir Simpson Hurricane Wind Scale (SSHWS) category 5 storm in the USA in the named era. The storm’s intensity, rapid intensification, October landfall, high inland winds, and uncommon landfall location all combined to complicate the communication and preparation efforts of emergency managers (EMs) and broadcast meteorologists (BMs), while clouding the comprehension of the public. Interviews were conducted with EMs, BMs, and a small public sample to hear their stories and identify and understand common themes and experiences. This information and previous research was used to inform the creation of questions for a large sample public survey. Results showed that 61% of our sample did not evacuate, and approximately 80% either underestimated the intensity, misinterpreted or did not believe the forecast, or realized the danger too late to evacuate. Hazard perception from a survey of the public revealed that wind followed by tornadoes, and falling trees were the major concerns across the region. According to their counties of residence, participants were divided into Coastal or Inland, and Heavily Impacted or Less Impacted categories. Inland participants expressed a significantly higher concern for wind, tornadoes, falling trees, and rainfall/inland flooding than Coastal participants. Participants from Heavily Impacted counties showed greater concern for storm surge, tornadoes, and falling trees than participants from Less Impacted counties. These results reinforce the continued need for all parties of the weather enterprise to strengthen communication capabilities with EMs and the public for extreme events.
Impact of Land Cover and Leaf Area Index on BVOC Emissions over the Korean Peninsula
Authors: Jang Youjung , Eo Yangdam , Jang Meongdo , Woo Jung-Hun , Kim Younha , Lee Jae-Bum , Lim Jae-Hyun
Published: July 2020

Biogenic volatile organic compound (BVOCs) emissions are the largest VOC emission source globally, and are precursors to ozone and secondary organic aerosols, both of which are strong, short-lived climate pollutants. BVOC emissions are usually estimated using the Model of Emissions of Gases and Aerosols from Nature (MEGAN), which requires Plant Functional Types (PFTs) and Leaf Area Indexes (LAIs) as inputs. Herein, the effects of refined input data on regional BVOC emission estimates are analyzed. For LAIs, lower resolution MODerate-resolution Imaging Spectroradiometer (MODIS), and higher spatio-temporal resolution Spatial and Temporal Adaptive Reflectance Fusion Model (STARFM) LAI were generated. For PFTs, local land cover maps were developed, in addition to MODIS PFT. In South Korea, annual emissions of isoprene and monoterpenes in 2015 were estimated as 384 and 160 Gg/year, respectively, using STARFM LAI and Local PFT (Case 4). For North Korea, 340 Gg/year isoprene and 72 Gg/year monoterpenes emissions were estimated using STARFM LAI and MODIS PFT. These estimates were 14–110% higher than when using MODIS LAI and MODIS PFT (Case 1). Inter-comparison with satellite-based inverse isoprene emission estimates from GlobEmission shows 32% (North Korea) to 34% (South Korea) overestimation in bottom-up data. Our new vegetation inputs improve MEGAN performance and resulting BVOC emission estimations. Performance of Weather Research and Forecasting (WRF) meteorological modeling requires improvement, especially for solar radiation, to avoid overestimation of isoprene emissions.
Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations against Ulcerative Colitis in Rats as Revealed via Biochemical and Metabolomic Assays
Authors: Abdallah Heba M.I. , Ammar Naglaa M. , Abdelhameed Mohamed F. , Gendy Abd El-Nasser G. El , Ragab Tamer I. M. , Abd-ElGawad Ahmed M. , Farag Mohamed A. , Alwahibi Mona S. , Elshamy Abdelsamed I.
Published: July 2020

Ulcerative colitis (UC) is a relapsing inflammatory disease of unknown etiology. The increased risk of cancer in UC patients warrants for the development of novel drug treatments. Herein, this work concerns with the investigation of the protective effects of Acacia saligna butanol extract (ASBE) and its nanoformulations on UC in a rat model and its underlying mechanism. Colitis was induced by slow intrarectal infusion of 2 mL of 4% (v/v in 0.9% saline) acetic acid. Colon samples were evaluated macroscopically, microscopically, and assayed for pro-inflammatory cytokine levels. To monitor associated metabolic changes in acetic acid-induced UC model, serum samples were analyzed for primary metabolites using GC–MS followed by multivariate data analyses. Treatment with ASBE attenuated acetic acid-induced UC as revealed by reduction of colon weight, ulcer area, and ulcer index. ASBE treatment also reduced Cyclooxygenase-2 (COX-2), Prostaglandin E2 (PGE2) & Interleukin-1β (IL-1β) levels in the inflamed colon. The nano-formulation of ASBE showed better protection than the crude extract against ulcer indices, increased PGE2 production, and histopathological alterations such as intestinal mucosal lesions and inflammatory infiltration. Distinct metabolite changes were recorded in colitis rats including a decrease in oleamide and arachidonic acid along with increased levels of lactic acid, fructose, and pyroglutamic acid. Treatment with nano extract restored metabolite levels to normal and suggests that cytokine levels were regulated by nano extract in UC. Conclusion: ASBE nano extract mitigated against acetic acid-induced colitis in rats, and the underlying mechanism could be attributed to the modulatory effects of ASBE on the inflammatory cascades. The applicability of metabolomics developed in this rat model seems to be crucial for evaluating the anti-inflammatory mechanisms of new therapeutics for acute colitis.
The Possibilities of Immunotherapy for Children with Primary Immunodeficiencies Associated with Cancers
Authors: Baleydier Frederic , Bernard Fanette , Ansari Marc
Published: July 2020

Many primary immunodeficiencies (PIDs) are recognised as being associated with malignancies, particularly lymphoid malignancies, which represent the highest proportion of cancers occurring in conjunction with this underlying condition. When patients present with genetic errors of immunity, clinicians must often reflect on whether to manage antitumoral treatment conventionally or to take a more personalised approach, considering possible existing comorbidities and the underlying status of immunodeficiency. Recent advances in antitumoral immunotherapies, such as monoclonal antibodies, antigen-specific adoptive cell therapies or compounds with targeted effects, potentially offer significant opportunities for optimising treatment for those patients, especially with lymphoid malignancies. In cases involving PIDs, variable oncogenic mechanisms exist, and opportunities for antitumoral immunotherapies can be considered accordingly. In cases involving a DNA repair defect or genetic instability, monoclonal antibodies can be proposed instead of chemotherapy to avoid severe toxicity. Malignancies secondary to uncontrolled virus-driven proliferation or the loss of antitumoral immunosurveillance may benefit from antivirus cell therapies or allogeneic stem cell transplantation in order to restore the immune antitumoral caretaker function. A subset of PIDs is caused by gene defects affecting targetable signalling pathways directly involved in the oncogenic process, such as the constitutive activation of phosphoinositol 3-kinase/protein kinase B (PI3K/AKT) in activated phosphoinositide 3-kinase delta syndrome (APDS), which can be settled with PI3K/AKT inhibitors. Therefore, immunotherapy provides clinicians with interesting antitumoral therapeutic weapons to treat malignancies when there is an underlying PID.
Erratum: Majumder, S. et al. Zinc Oxide Nanoparticles Functionalized on Hydrogel Grafted Silk Fibroin Fabrics as Efficient Composite Dressing. Biomolecules 2020, 10, 710
Authors: Majumder Sudip , Ranjan Dahiya Ujjwal , Yadav Sunny , Sharma Pratibha , Ghosh Debashree , Rao Gyandshwar K. , Rawat Varun , Kumar Gaurav , Kumar Anuj , Srivastava Chandra Mohan
Published: July 2020

The authors wish to make the following correction to this paper [...]
Curcumin Nanoparticles and Their Cytotoxicity in Docetaxel-Resistant Castration-Resistant Prostate Cancer Cells
Authors: Tanaudommongkon Irin , Tanaudommongkon Asama , Prathipati Priyanka , Nguyen Joey Trieu , Keller Evan T. , Dong Xiaowei
Published: July 2020

Most prostate cancer patients develop resistance to anti-androgen therapy. This is referred to as castration-resistant prostate cancer (CRPC). Docetaxel (DTX) is the mainstay treatment against CRPC. However, over time patients eventually develop DTX resistance, which is the cause of the cancer-related mortality. Curcumin (CUR) as a natural compound has been shown to have very broad pharmacological activities, e.g., anti-inflammatory and antioxidant properties. However, CUR is very hydrophobic. The objective of this study was to develop CUR nanoparticles (NPs) and evaluate their cytotoxicity in DTX-resistant CRPC cells for the treatment of DTX-resistant CRPC. We tested solubility of CUR in different lipids and surfactants. Finally, Miglyol 812 and D-alpha-tocopheryl poly (ethylene glycol) succinate 1000 (TPGS) were chosen to prepare lipid-based NPs for CUR. We fully characterized CUR NPs that had particle size < 150 nm, high drug loading (7.5%), and entrapment efficiency (90%). Moreover, the CUR NPs were successfully lyophilized without using cryoprotectants. We tested the cytotoxicity of blank NPs, free CUR, and CUR NPs in sensitive DU145 and PC3 cells as well as their matching docetaxel-resistant cells. Cytotoxicity studies showed that blank NPs were very safe for all tested prostate cancer cell lines. Free CUR overcame the resistance in PC3 cells, but not in DU145 cells. In contrast, CUR NPs significantly increased CUR potency in all tested cell lines. Importantly, CUR NPs completely restored CUR potency in both resistant DU145 and PC3 cells. These results demonstrate that the CUR NPs have potential to overcome DTX resistance in CRPC.
A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy
Authors: Guzman Norberto A. , Guzman Daniel E.
Published: July 2020

Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, and expensive. To address these limitations, we have developed a prototype of a portable, miniaturized instrument that uses immunoaffinity capillary electrophoresis (IACE) to isolate, concentrate, and analyze cell-free biomarkers and/or tissue or cell extracts present in biological fluids. Isolation and concentration of analytes is accomplished through binding to one or more biorecognition affinity ligands immobilized to a solid support, while separation and analysis are achieved by high-resolution capillary electrophoresis (CE) coupled to one or more detectors. When compared to other existing methods, the process of this affinity capture, enrichment, release, and separation of one or a panel of biomarkers can be carried out on-line with the advantages of being rapid, automated, and cost-effective. Additionally, it has the potential to demonstrate high analytical sensitivity, specificity, and selectivity. As the potential of liquid biopsy grows, so too does the demand for technical advances. In this review, we therefore discuss applications and limitations of liquid biopsy and hope to introduce the idea that our affinity capture-separation device could be used as a form of point-of-care (POC) diagnostic technology to isolate, concentrate, and analyze circulating cells, extracellular vesicles, and viruses.