bluuebunny/crossref_metadata_2025 huggingface dataset:
Energy efficient data encoding in DRAM channels exploiting data value similarity
Authors: Seol Hoseok , Shin Wongyu , Jang Jaemin , Choi Jungwhan , Suh Jinwoong , Kim Lee-Sup
Published: October 2016

As DRAM data bandwidth increases, tremendous energy is dissipated in the DRAM data bus. To reduce the energy consumed in the data bus, DRAM interfaces with asymmetric termination, such as Pseudo Open Drain (POD) and Low Voltage Swing Terminated Logic (LVSTL), have been adopted in modern DRAMs. In interfaces using asymmetric termination, the amount of termination energy is proportional to the hamming weight of the data words. In this work, we propose Bitwise Difference Encoding (BD-Encoding), which decreases the hamming weight of data words, leading to a reduction in energy consumption in the modern DRAM data bus. Since smaller hamming weight of the data words also reduces switching activity, switching energy and power noise are also both reduced.

BD-Encoding exploits the similarity in data words in the DRAM data bus. We observed that similar data words (i.e. data words whose hamming distance is small) are highly likely to be sent over at similar times. Based on this observation, BD-coder stores the data recently sent over in both the memory controller and DRAMs. Then, BD-coder transfers the bitwise difference between the current data and the most similar data. In an evaluation using SPEC 2006, BD-Encoding using 64 recent data reduced termination energy by 58.3% and switching energy by 45.3%. In addition, 55% of the LdI/dt noise was decreased with BD-Encoding.
Novel developments in differentiating the role of renal and intestinal sodium hydrogen exchanger 3
Authors: Dominguez Rieg Jessica A. , de la Mora Chavez Samantha , Rieg Timo
Published: October 2016

The Na+/H+exchanger isoform 3 (NHE3) facilitates Na+absorption and H+secretion and is expressed in the intestine, proximal tubule, and thick ascending limb of the kidney. While the function of NHE3 for Na+and [Formula: see text](re)absorption has been defined using conventional NHE3 knockout mice (NHE3−/−), the recent generation of conditional NHE3 knockout mice started to give critical new insight into the role of this protein by allowing for temporal and spatial control of NHE3 expression. For example, in contrast to NHE3−/−mice, knockout of NHE3 in the S1 and S2 segments of the proximal tubule or along the entire tubule/collecting duct does not cause any lethality. Nonabsorbable NHE3 inhibitors have been developed, and preclinical as well as clinical trials indicate possible pharmacological use in fluid overload, hypertension, chronic kidney disease, hyperphosphatemia, and constipation. Some of the therapeutic considerations seem to be directly related to the pharmacodynamic properties of these drugs; however, little is known about the effects of these nonabsorbable NHE3 inhibitors on intestinal phosphate transport and the mechanisms so far remain elusive. This review focuses on novel findings of NHE3 in the intestine and the kidney as well as novel drug developments targeting NHE3.
Depletion of vacuolar protein sorting-associated protein 35 is associated with increased lysosomal degradation of aquaporin-2
Authors: Lee Mi Suk , Choi Hyo-Jung , Park Eui-Jung , Park Hye-Jeong , Kwon Tae-Hwan
Published: October 2016

The carboxyl terminus of aquaporin-2 (AQP2c) undergoes posttranslational modifications, including phosphorylation and ubiquitination, in the process of regulating aquaporin-2 (AQP2) translocation and protein abundance. We aimed to identify novel proteins interacting with AQP2c. Recombinant AQP2c protein was made in Escherichia coli BL21 (DE3) cells by exploiting the pET32 TrxA fusion system. Lysates of rat kidney inner medullary collecting duct (IMCD) tubule suspensions interacted with rat AQP2c bound to Ni2+-resin were subjected to LC-MS/MS proteomic analysis. Potential interacting proteins were identified, including vacuolar protein sorting-associated protein 35 (Vps35). Coimmunoprecipitation assay demonstrated that Vps35 interacted with AQP2c. Immunohistochemistry of rat kidney revealed that AQP2 and Vps35 were partly colocalized at the intracellular vesicles in collecting duct cells. The role of Vps35 in AQP2 regulation induced by 1-deamino-8D-arginine vasopressin (dDAVP) was examined in mpkCCDc14 cells. Cell surface biotinylation assay demonstrated that dDAVP-induced apical translocation of AQP2 was significantly decreased under siRNA-mediated Vps35 knockdown. dDAVP-induced AQP2 upregulation was less prominent in the cells with Vps35 knockdown. Moreover, AQP2 protein abundance was decreased to a greater extent during the withdrawal period after dDAVP stimulation under Vps35 knockdown, which was significantly inhibited by chloroquine (a blocker of the lysosomal pathway) but not by MG132 (a proteasome inhibitor). Immunocytochemistry demonstrated that internalized AQP2 was more associated with lysosomal-associated membrane protein 1 (LAMP-1) in primary cultured IMCD cells under a Vps35 knockdown situation. Taken together, our results show that Vps35 interacts with AQP2c, and depletion of Vps35 is likely to be associated with decreased AQP2 trafficking and increased lysosomal degradation of AQP2 protein.
Long-term aldosterone administration increases renal Na+-Cl−cotransporter abundance in late distal convoluted tubule
Authors: Poulsen Søren Brandt , Christensen Birgitte Mønster
Published: October 2016

Renal Na+-Cl−cotransporter (NCC) is expressed in early distal convoluted tubule (DCT) 1 and late DCT (DCT2). NCC activity can be stimulated by aldosterone administration, and the mechanism is assumed to depend on the enzyme 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), which inactivates glucocorticoids that would otherwise occupy aldosterone receptors. Because 11β-HSD2 in rat may only be abundantly expressed in DCT2 cells and not in DCT1 cells, it has been speculated that aldosterone specifically stimulates NCC activity in DCT2 cells. In mice, however, it is debated if 11β-HSD2 is expressed in DCT2 cells. The present study examined whether aldosterone administration in mice stimulates NCC abundance and phosphorylation in DCT2 cells but not in DCT1 cells. B6/C57 male mice were administered 100 µg aldosterone·kg body weight−1·24 h−1for 6 days and euthanized during isoflurane inhalation. Western blotting of whole kidney homogenate showed that aldosterone administration stimulated NCC and pT58-NCC abundances ( P < 0.001). In DCT1 cells, confocal microscopy detected no effect of the aldosterone administration on NCC and pT58-NCC abundances. By contrast, NCC and pT58-NCC abundances were stimulated by aldosterone administration in the middle of DCT2 ( P < 0.001 and <0.01, respectively) and at the junction between DCT2 and CNT ( P < 0.001 and <0.05, respectively). In contrast to rat, immunohistochemistry in mouse showed no/very weak 11β-HSD2 expression in DCT2 cells. Collectively, long-term aldosterone administration stimulates mouse NCC and pT58-NCC abundances in DCT2 cells and presumably not in DCT1 cells.
The relationship between urinary renin-angiotensin system markers, renal function, and blood pressure in adolescents with type 1 diabetes
Authors: Burns Kevin D. , Lytvyn Yuliya , Mahmud Farid H. , Daneman Denis , Deda Livia , Dunger David B. , Deanfield John , Dalton R. Neil , Elia Yesmino , Har Ronnie , Van Julie A.D. , Bradley Timothy J. , Slorach Cameron , Hui Wei , Xiao Fengxia , Zimpelmann Joseph , Mertens Luc , Moineddin Rahim , Reich Heather N. , Sochett Etienne , Scholey James W. , Cherney David Z. I.
Published: October 2016

The relationship between the renal renin-angiotensin aldosterone system (RAAS) and cardiorenal pathophysiology is unclear. Our aims were to assess 1) levels of urinary RAAS components and 2) the association between RAAS components and HbA1c, the urine albumin/creatinine ratio (ACR), estimated glomerular filtration rate (eGFR), and blood pressure (BP) in otherwise healthy adolescents with type 1 diabetes mellitus (TID) vs. healthy controls (HC). Urinary angiotensinogen and angtionsin-converting enzyme (ACE) 2 levels, activity of ACE and ACE2, BP, HbA1c, ACR, and eGFR were measured in 65 HC and 194 T1D from the Adolescent Type 1 Diabetes Cardio-Renal Intervention Trial (AdDIT). Urinary levels of all RAAS components were higher in T1D vs. HC ( P < 0.0001). Higher HbA1c was associated with higher urinary angiotensinogen, ACE2, and higher activity of ACE and ACE2 ( P < 0.0001, P = 0.0003, P = 0.003, and P = 0.007 respectively) in T1D. Higher ACR (within the normal range) was associated with higher urinary angiotensinogen ( P < 0.0001) and ACE activity ( P = 0.007), but not with urinary ACE2 activity or ACE2 levels. These observations were absent in HC. Urinary RAAS components were not associated with BP or eGFR in T1D or HC. Otherwise healthy adolescents with T1D exhibit higher levels of urinary RAAS components compared with HC. While levels of all urinary RAAS components correlate with HbA1c in T1D, only urinary angiotensinogen and ACE activity correlate with ACR, suggesting that these factors reflect an intermediary pathogenic link between hyperglycemia and albuminuria within the normal range.
Minimally Invasive Methods for Staging in Lung Cancer: Systematic Review and Meta-Analysis
Authors: Labarca Gonzalo , Aravena Carlos , Ortega Francisco , Arenas Alex , Majid Adnan , Folch Erik , Mehta Hiren J. , Jantz Michael A. , Fernandez-Bussy Sebastian
Published: October 2016

Introduction. Endobronchial ultrasound (EBUS) is a procedure that provides access to the mediastinal staging; however, EBUS cannot be used to stage all of the nodes in the mediastinum. In these cases, endoscopic ultrasound (EUS) is used for complete staging.Objective. To provide a synthesis of the evidence on the diagnostic performance of EBUS + EUS in patients undergoing mediastinal staging.Methods. Systematic review and meta-analysis to evaluate the diagnostic yield of EBUS + EUS compared with surgical staging. Two researchers performed the literature search, quality assessments, data extractions, and analyses. We produced a meta-analysis including sensitivity, specificity, and likelihood ratio analysis.Results. Twelve primary studies (1515 patients) were included; two were randomized controlled trials (RCTs) and ten were prospective trials. The pooled sensitivity for combined EBUS + EUS was 87% (CI 84–89%) and the specificity was 99% (CI 98–100%). For EBUS + EUS performed with a single bronchoscope group, the sensitivity improved to 88% (CI 83.1–91.4%) and specificity improved to 100% (CI 99-100%).Conclusion. EBUS + EUS is a highly accurate and safe procedure. The combined procedure should be considered in selected patients with lymphadenopathy noted at stations that are not traditionally accessible with conventional EBUS.
Extrinsic and Intrinsic Regulation of Axon Regeneration by MicroRNAs after Spinal Cord Injury
Authors: Li Ping , Teng Zhao-Qian , Liu Chang-Mei
Published: October 2016

Spinal cord injury is a devastating disease which disrupts the connections between the brain and spinal cord, often resulting in the loss of sensory and motor function below the lesion site. Most injured neurons fail to regenerate in the central nervous system after injury. Multiple intrinsic and extrinsic factors contribute to the general failure of axonal regeneration after injury. MicroRNAs can modulate multiple genes’ expression and are tightly controlled during nerve development or the injury process. Evidence has demonstrated that microRNAs and their signaling pathways play important roles in mediating axon regeneration and glial scar formation after spinal cord injury. This article reviews the role and mechanism of differentially expressed microRNAs in regulating axon regeneration and glial scar formation after spinal cord injury, as well as their therapeutic potential for promoting axonal regeneration and repair of the injured spinal cord.
Normocomplementaemic Urticarial Vasculitis in a 19-Month-Old Girl
Authors: Williams Peter
Published: October 2016

Urticaria is common in children. Urticarial vasculitis (UV) is a potentially more serious, rare variant. The youngest reported case was 12 months of age. A systemically well, 19-month-old girl presented with her mother who was concerned about the development of a rash. On presentation, the child had normal vital signs, was alert, and was well and playing with toys. There was a widespread urticarial rash (raised, pruritic, and erythematous) that was most apparent on the trunk with minimal rash on the legs. Overlying this urticarial rash in a similar distribution was a blotchy, palpable purpuric rash and associated hyperpigmentation. Investigations revealed a normal level of haemoglobin, white cells, platelets, and electrolytes. Renal function, international normalised ratio, and activated partial thromboplastin time were all normal. There was no blood or protein in the urine. The erythrocyte sedimentation rate was mildly elevated at 19 mm/hour. Complement results (including C1q) obtained later were normal. This case is striking not only because of the rarity of UV in children but also due to the unique diagnostic and prognostic challenges that it raises.
The Role of the Transcription Factor Foxo3 in Hearing Maintenance: Informed Speculation on a New Player in the Cochlea
Authors: White Patricia M.
Published: October 2016

Molecular genetics has proven to be a powerful approach for understanding early-onset hearing loss. Recent work in late-onset hearing loss uses mouse genetics to identify molecular mechanisms that promote the maintenance of hearing. One such gene, Foxo3, is ontologically involved in preserving mitochondrial function. Significant evidence exists to support the idea that mitochondrial dysfunction is correlated with and can be causal for hearing loss. Foxo3 is also ontologically implicated in driving the circadian cycle, which has recently been shown to influence the molecular response to noise damage. In this review, the molecular framework connecting these cellular processes is discussed in relation to the cellular pathologies observed in human specimens of late-onset hearing loss. In bringing these observations together, the possibility arises that distinct molecular mechanisms work in multiple cell types to preserve hearing. This diversity offers great opportunities to understand and manipulate genetic processes for therapeutic gain.
Tortuous Common Carotid Artery: A Report of Four Cases Observed in Cadaveric Dissections
Authors: Iwanaga Joe , Watanabe Koichi , Tsuyoshi Saga , Tabira Yoko , Yamaki Koh-ichi
Published: October 2016

A tortuous common carotid artery poses a high risk of injury during tracheotomy. Preoperative diagnosis is therefore important to avoid serious complications. We found four cases of tortuous common carotid artery during an anatomical dissection course for students. The first case was a 91-year-old woman who had bilateral tortuous common carotid arteries without arteriosclerosis. Case2was a 78-year-old woman who had bilateral tortuous common carotid arteries without arteriosclerosis. Case3was an 86-year-old woman who died from bladder cancer and who also had a right tortuous common carotid artery without arteriosclerosis. Case4was an 89-year-old woman who had bilateral tortuous common carotid arteries and a tortuous brachiocephalic artery with severe arteriosclerosis. Case4was also examined using computed tomography to evaluate the arteriosclerosis. Computed tomography revealed severe calcification of the vascular wall, which was confirmed in the aortic arch and origins of its branches. In all four cases, the tortuosity was located below the level of the thyroid gland. Based on prior study results indicating that fusion between the carotid sheath and visceral fascia was often evident at the level of the thyroid gland, we speculated that the major region in which tortuosity occurs is at the same level or inferior to the level of the thyroid gland.
Efficacy and Safety of Chinese Medicinal Herbs for the Treatment of Hyperuricemia: A Systematic Review and Meta‐Analysis
Authors: Lin Jianping , Chen Shaoqing , Li Shuzhen , Lu Meili , Li Yanan , Su Youxin
Published: October 2016

Background. Chinese medicinal herbs may be useful for the treatment of hyperuricemia, but there has been no systematic assessment of their efficacy and safety. Objectives. To systematically assess the efficacy and safety of Chinese medicinal herbs for the treatment of hyperuricemia. Methods. Six electronic databases were searched from their inception to December 2015. Randomized controlled clinical trials (RCTs) were included. Cochrane criteria were applied to assess the risk of bias. Data analysis was performed using RevMan software version 5.2. Results. Eleven RCTs with 838 patients were included. There was no significant difference in serum uric acid between Chinese medicinal herbs and traditional Western medicine (SME: 0.19, 95% CI: −0.04 to 0.43; p = 0.10). In terms of overall efficacy, the Chinese medicinal herbs were significantly superior to Western medicine (RR: 1.11; 95% CI: 1.04 to 1.17; p = 0.0007). The Chinese medicinal herbs were better than Western medicine in reducing the adverse reactions (RR: 0.30; 95% CI: 0.15 to 0.62; p = 0.001). And all these funnel plots showed unlikelihood of publishing bias. Conclusions. The results indicate that Chinese medicinal herbs may have greater overall efficacy with fewer adverse drug reactions, although the evidence is weak owing to the low methodological quality and the small number of the included trials.
Diastema Closure in Anterior Teeth Using a Posterior Matrix
Authors: Goyal Ayush , Nikhil Vineeta , Singh Ritu
Published: October 2016

Presence of diastema between anterior teeth is often considered an onerous esthetic problem. Various treatment modalities are available for diastema closure. However, not all diastemas can be treated the same in terms of modality or timing. The extent and the etiology of the diastema must be properly evaluated. Proper case selection is of paramount importance for a successful treatment. In this case report, diastema closure was performed with direct composite restorations. One bottle etch-and-rinse adhesive was used and a single shade was used to close the diastemas. Contoured sectional posterior matrix was used to achieve anatomic contouring of the proximal surfaces of the teeth. This was followed by finishing and polishing using polishing discs. Patient was kept on recall every 6 months.Conclusion. Diastema closure with correct anatomic contouring is easy to perform using the contoured sectional matrices. At 14-month recall, no clinical signs of failure like discoloration or fracture were evident. Also, patient did not complain of any sensitivity. Thus, direct composite restorations serve as durable and highly esthetic restorations leading to complete patient satisfaction.
On the Jeans Theorem and the “Tolman–Oppenheimer–Volkoff Equation” inR2Gravity
Authors: Jain Rishabh , Sidharth Burra G. , Corda Christian
Published: October 2016

Corda, Mosquera Cuesta, and Lorduy Gòmez have shown that spherically symmetric stationary states can be used as a model for galaxies in the framework of the linearizedR2gravity. Those states could represent a partial solution to the Dark Matter Problem. Here, we discuss an improvement of this work. In fact, as the star density is a functional of the invariants of the associated Vlasov equation, we show that any of these invariants is in its turn a functional of the local energy and the angular momentum. As a consequence, the star density depends only on these two integrals of the Vlasov system. This result is known as the “Jeans theorem.” In addition, we find an analogy of the historical Tolman–Oppenheimer–Volkoff equation for the system considered in this paper. For the sake of completeness, in the final section of the paper, we consider two additional models which argue that Dark Matter could not be an essential element.
Link-Polymorphism of 5-HTT Promoter Region Is Associated with Autoantibodies in Patients with Systemic Lupus Erythematosus
Authors: Li Shu , Liu Shuang , Chen Fan , Cheng Yuqi , Bai Ru , Lai Aiyun , Lu Zhaoping , Xu Jian
Published: October 2016

Serotonin transporter linked polymorphic region (5-HTTLPR) was reported to associate with depression in systemic lupus erythematosus (SLE) patients by our team. To explore whether 5-HTTLPR plays a role in the pathogenesis of SLE, we tested 138 SLE patients and 138 age and sex matched health controls (HCs) for 5-HTTLPR by polymerase chain reaction (PCR) and agarose gel electrophoresis. Interestingly, the results suggest that the frequencies of SS genotype and S allele in SLE patients with positive anti-Sm antibody and anti-U1RNP antibody were both significantly higher than the other genotypes and alleles. However, the frequencies of 5-HTTLPR genotypes and alleles were of no significant difference between SLE patients and HCs. This suggested that 5-HTTLPR was not a high-risk susceptible gene in SLE but might relate to SLE by affecting production of some autoantibodies, especially anti-Sm and anti-U1RNP antibody.
Epigenetic Remodeling in Male Germline Development
Authors: Li Na , Shen Qiaoyan , Hua Jinlian
Published: October 2016

In mammals, germ cells guarantee the inheritance of genetic and epigenetic information across generations and are the origin of a new organism. During embryo development, the blastocyst is formed in the early stage, is comprised of an inner cell mass which is pluripotent, and could give rise to the embryonic stem cells (ESCs). The inner cell mass undergoes demethylation processes and will reestablish a methylated state that is similar to that of somatic cells later in epiblast stage. Primordial germ cells (PGCs) will be formed very soon and accompanied by the process of genome‐wide demethylation. With the input of male sex determination genes, spermatogonial stem cells (SSCs) are generated and undergo the process of spermatogenesis. Spermatogenesis is a delicately regulated process in which various regulations are launched to guarantee normal mitosis and meiosis in SSCs. During all these processes, especially during spermatid development, DNA methylation profile and histone modifications are of crucial importance. In this review, we will discuss the epigenetic modifications from zygote formation to mature sperm generation and their significance to these development processes.
Interspinous Process Decompression: Expanding Treatment Options for Lumbar Spinal Stenosis
Authors: Nunley Pierce D. , Shamie A. Nick , Blumenthal Scott L. , Orndorff Douglas , Block Jon E. , Geisler Fred H.
Published: October 2016

Interspinous process decompression is a minimally invasive implantation procedure employing a stand-alone interspinous spacer that functions as an extension blocker to prevent compression of neural elements without direct surgical removal of tissue adjacent to the nerves. The Superion® spacer is the only FDA approved stand-alone device available in the US. It is also the only spacer approved by the CMS to be implanted in an ambulatory surgery center. We computed the within-group effect sizes from the Superion IDE trial and compared them to results extrapolated from two randomized trials of decompressive laminectomy. For the ODI, effect sizes were allvery large(>1.0) for Superion and laminectomy at 2, 3, and 4 years. For ZCQ, the 2-year Superion symptom severity (1.26) and physical function (1.29) domains werevery large; laminectomy effect sizes werevery large(1.07) for symptom severity andlargefor physical function (0.80). Current projections indicate a marked increase in the number of patients with spinal stenosis. Consequently, there remains a keen interest in minimally invasive treatment options that delay or obviate the need for invasive surgical procedures, such as decompressive laminectomy or fusion. Stand-alone interspinous spacers may fill a currently unmet treatment gap in the continuum of care and help to reduce the burden of this chronic degenerative condition on the health care system.
Primary Sjogren’s Syndrome Presenting as Acute Interstitial Pneumonitis/Hamman-Rich Syndrome
Authors: Khan Abidullah , Humayun Mohammad , Haider Iqbal , Ayub Maimoona , Shah Zakir , Ajmal Fahad
Published: October 2016

A previously well, 45-year-old Pakistani lady was admitted to the medical unit on-call of Khyber Teaching Hospital (KTH) Peshawar with a 5-day history of fever, productive cough with copious mucoid sputum, dyspnea, and pleuritic chest pain. She also complained of dry eyes, mouth, and vagina. Her chest X-ray showed diffuse alveolar shadowing and arterial gas analysis confirmed type 1 respiratory failure. Over the next few days, she deteriorated rapidly making an urgent transfer to the medical intensive care unit (MICU) necessary, where she was mechanically ventilated. An HRCT followed by bronchoscopic biopsies made a diagnosis of acute interstitial pneumonitis (AIP), formerly known as Hamman-Rich syndrome. She also turned out to be positive for both anti-SS-A/Ro and anti-SS-B/La antibodies along with a positive Schirmer’s test and lower lip biopsy. She received intravenous steroids and supportive care. The patient had a complete recovery after approximately three weeks’ stay in the hospital with lung function returning back to normal. This is most probably the first ever case of primary Sjogren syndrome (pSjS) presenting as AIP, recovering completely in less than a month time.
Free-Standing Porous Carbon Nanofiber Networks from Electrospinning Polyimide for Supercapacitors
Authors: Wang Bo , Lu Gang , Luo Qiu-Ping , Wang Tianhu
Published: October 2016

Free-standing porous carbon nanofiber networks (CFNs) were synthesized by electrospinning method and carbonization procedure. We study the implementation of porous CFNs as supercapacitor electrodes and electrochemical measurements demonstrated that porous CFNs exhibit a specific capacitance (205 F/g at the scan rate of 5 mV/s) with high flexibility and good rate capability performance (more than 70% of its initial capacitance from 5 mV/s to 200 mV/s). Furthermore, porous CFNs exhibited an excellent cycling stability (just 12% capacitance loss after 10,000 cycles). These results suggest that porous CFNs are very promising candidates as flexible supercapacitor electrodes.
Analytical Analysis and Field Observation of Break Line in the Main Roof over the Goaf Edge of Longwall Coal Mines
Authors: Guangchao Zhang , Fulian He , Lishuai Jiang
Published: October 2016

This paper presents an integrated approach for analytical analysis and field tests to estimate the break line in a main roof over the goaf edge. An analytical model which treated the main roof as a beam seating on the Winkler foundation and subjected to nonuniformity roof loading was established. Further analysis of the bending moment distribution of such a main roof beam was undertaken. Based on the geological conditions pertaining to a case study at Wangjialing coal mine, Shanxi Province, China, the break line in the main roof in a typical longwall panel was calculated in the rib-sides at a distance of 5.6 to 7.4 m from the goaf edge. The influence of main roof flexural rigidity and foundation rigidity and so forth on the bending moment distribution was revealed by a parametric study. Borehole camera detection was employed to further validate the analytical model and its results. The results of the field test demonstrated that the break line detected in the main roof was about 5.5 to 6.8 m away from the goaf edge, which was in good agreement with the analytical model.
Comparison of Perineal Sonographically Measured and Functional Urodynamic Urethral Length in Female Urinary Incontinence
Authors: Najjari Laila , Janetzki Nadine , Kennes Lieven , Stickeler Elmar , Serno Julia , Behrendt Julia
Published: October 2016

Objectives. To detect the anatomical insufficiency of the urethra and to propose perineal ultrasound as a useful, noninvasive tool for the evaluation of incontinence, we compared the anatomical length of the urethra with the urodynamic functional urethral length. We also compared the urethral length between continent and incontinent females.Methods. 149 female patients were enrolled and divided into four groups (stress, urge, or mixed incontinence; control). Sonographically measured urethral length (SUL) and urodynamic functional urethral length (FUL) were analyzed statistically. Standardized and internationally validated incontinence questionnaire ICIQ-SF results were compared between each patient group.Results. Perineal SUL was significantly longer in incontinent compared to continent patients (p<0.0001). Pairwise comparison of each incontinent type (stress, urge, or mixed incontinence) with the control group showed also a significant difference (p<0.05). FUL was significantly shorter in incontinent patients than in the control group (p=0.0112). But pairwise comparison showed only a significant difference for the stress incontinence group compared with the control group (p=0.0084) and not for the urge or mixed incontinent group. No clear correlation between SUL, FUL, and ICIQ-SF score was found.Conclusions. SUL measured by noninvasive perineal ultrasound is a suitable parameter in the assessment of female incontinence, since incontinent women show a significantly elongated urethra as a sign of tissue insufficiency, independent of the type of incontinence.
Reliability Analysis of the Chatter Stability during Milling Using a Neural Network
Authors: Hu Sen , Huang Xianzhen , Zhang Yimin , Lv Chunmei
Published: October 2016

The parameters of a system have the randomness generally in the process of milling, which influences the stability of the milling. This paper uses the neural network to get a comprehensive analysis of the influences of random factors in milling and proposes a method for reliability analysis of the regenerative chatter stability in milling. Dynamic model of milling regenerative chatter is established, and stability lobe diagram is obtained by the full-discretization method (FDM). The neural network is applied to approximate the functional relationship of the limit axial cutting depth; then the reliability is computed with the Monte Carlo simulation method (MCSM) and the moment method (MM), respectively. Finally, the results of an example are used to demonstrate the efficiency and accuracy of the proposed method.
Current Trends in Neuromuscular Blockade, Management, and Monitoring amongst Singaporean Anaesthetists
Authors: Teoh Wendy H. , Ledowski Thomas , Tseng Phillip S.
Published: October 2016

Introduction. This survey aimed to investigate the attitudes/practice pertaining the use, management, and monitoring of neuromuscular blockade amongst Singaporean anaesthetists.Methods. All specialist accredited anaesthetists registered with the Singapore Medical Council were invited to complete an anonymous online survey.Results. The response rate was 39.5%. Neuromuscular monitoring (NM) was used routinely by only 13.1% despite the widespread availability of monitors. 82% stated residual NMB (RNMB) was a significant risk factor for patient outcome, but only 24% believed NMB monitoring should be compulsory in all paralyzed patients. 63.6% of anaesthetists estimated the risk of RNMB in their own institutions to be <5%. 63.1% always gave reversal. Neostigmine was predominantly used (85.1%), with 28.2% using sugammadex at least sometimes, citing unavailability and high costs. However, 83.8% believed in sugammadex’s benefits for patients’ safety and >50% said such benefits may be able to offset the associated costs.Conclusions. There is a significant need for reeducation about RNMB, studies on local RNMB incidences, and strengthening of current monitoring practices and guidelines. Strategies are discussed. As NM monitors appear widely available and reversal of NMB standard practice, it is hopeful that Singaporean anaesthetists will change and strive for evidence-based best clinical practice to enhance patient safety.
Implant Composed of Demineralized Bone and Mesenchymal Stem Cells Genetically Modified with AdBMP2/AdBMP7 for the Regeneration of Bone Fractures in Ovis aries
Authors: Hernandez-Hurtado Adelina A. , Borrego-Soto Gissela , Marino-Martinez Ivan A. , Lara-Arias Jorge , Romero-Diaz Viktor J. , Abrego-Guerra Adalberto , Vilchez-Cavazos Jose F. , Elizondo-Riojas Guillermo , Martinez-Rodriguez Herminia G. , Espinoza-Juarez Marcela A. , Lopez-Romero Gloria C. , Robles-Zamora Alejandro , Mendoza Lemus Oscar F. , Ortiz-Lopez Rocio , Rojas-Martinez Augusto
Published: October 2016

Adipose‐derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated the in vitro osteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep. This graft was compared to ewes implanted with untransduced ADMSCs embedded in the same matrix and with injured but untreated animals. In vivo results showed accelerated osteogenesis in the group treated with the AdBMP2/AdBMP7 transduced ADMSC graft, which also showed improved restoration of the normal bone morphology.
Development and Life History ofSitophilus zeamais(Coleoptera: Curculionidae) on Cereal Crops
Authors: Ojo James Adebayo , Omoloye Adebayo Amos
Published: October 2016

The maize weevil,Sitophilus zeamaisMotschulsky (Coleoptera: Curculionidae), is one of the most destructive pests of stored cereals. Knowledge of the life history and biology is important to the development of an integrated pest management program. Investigation was carried out on developmental biology ofS. zeamaison four main cereal crops, maize, rice, sorghum, and millet, under laboratory conditions. Egg incubation, oviposition periods, and larval instar development were not different significantly among the food hosts. Number of eggs laid varied significantly among the cereal grains; mean fecundity was highest on maize (67.2±3.16) and lowest on millet (53.8±0.17). Number of immature (larva and pupa) and adult stages varied significantly among the cereal grains. There exist four larval instars with a varied mean head capsule width, with a mean total instar larval developmental period of 23.1, 22.2, 22.2, and 21.6 d on maize, rice, sorghum, and millet, respectively. There was linear relationship and significant correlation between the stages of larval development and head capsule width. The mean developmental period from egg to adult varied, being highest on maize (34.7 d) and lowest on sorghum (33.5 d).
High-Throughput Analysis of the T Cell Receptor Beta Chain Repertoire in PBMCs from Chronic Hepatitis B Patients with HBeAg Seroconversion
Authors: Qu Yachao , Huang Yong , Liu Di , Huang Yinuo , Zhang Zhiyi , Mi Zhiqiang , An Xiaoping , Tong Yigang , Lu Jun
Published: October 2016

T lymphocytes are the most important immune cells that affect both the development and treatment of hepatitis B. We used high-throughput sequencing to determine the diversity in the V and J regions of the TCRβchain in 4 chronic hepatitis B patients before and after HBeAg seroconversion. Here, we demonstrate that the 4 patients expressedVβ12-4 at the highest frequencies of 10.6%, 9.2%, 17.5%, and 7.5%, andVβ28was the second most common, with frequencies of 7.8%, 6.7%, 5.3%, and 10.9%, respectively. No significant changes were observed following seroconversion. With regard to the Jβgene, Jβ2-1 was the most commonly expressed in the 4 patients at frequencies of 5.8%, 6.5%, 11.3%, and 7.3%, respectively. Analysis of the V-J region genes revealed several differences, including significant increases in the expression levels of V7-2-01-J2-1, V12-4-J1-1, and V28-1-J1-5 and a decrease in that of V19-01-J2-3. These results illustrate the presence of biased TCRVβand Jβgene expression in the chronic hepatitis B patients. TRBVβ12-4,Vβ28, Jβ2-1, V7-2-01-J2-1, V12-4-J1-1, and V28-1-J1-5 may be associated with the development and treatment of CHB.
Oxidative Stress during HIV Infection: Mechanisms and Consequences
Authors: Ivanov Alexander V. , Valuev-Elliston Vladimir T. , Ivanova Olga N. , Kochetkov Sergey N. , Starodubova Elizaveta S. , Bartosch Birke , Isaguliants Maria G.
Published: October 2016

It is generally acknowledged that reactive oxygen species (ROS) play crucial roles in a variety of natural processes in cells. If increased to levels which cannot be neutralized by the defense mechanisms, they damage biological molecules, alter their functions, and also act as signaling molecules thus generating a spectrum of pathologies. In this review, we summarize current data on oxidative stress markers associated with human immunodeficiency virus type‐1 (HIV‐1) infection, analyze mechanisms by which this virus triggers massive ROS production, and describe the status of various defense mechanisms of the infected host cell. In addition, we have scrutinized scarce data on the effect of ROS on HIV‐1 replication. Finally, we present current state of knowledge on the redox alterations as crucial factors of HIV‐1 pathogenicity, such as neurotoxicity and dementia, exhaustion of CD4+/CD8+ T‐cells, predisposition to lung infections, and certain side effects of the antiretroviral therapy, and compare them to the pathologies associated with the nitrosative stress.
Studies on Shokyo, Kanzo, and Keihi in Kakkonto Medicine on Prostaglandin E2Production in Lipopolysaccharide-Treated Human Gingival Fibroblasts
Authors: Ara Toshiaki , Sogawa Norio
Published: October 2016

We previously demonstrated that a kampo medicine, kakkonto, decreases lipopolysaccharide- (LPS-) induced prostaglandin E2(PGE2) production by human gingival fibroblasts. In this study, we examined the herbs constituting kakkonto that exhibit this effect. Shokyo strongly and concentration dependently and kanzo and keihi moderately decreased LPS-induced PGE2production. Shokyo did not alter cyclooxygenase-2 (COX-2) activity, cytosolic phospholipase A2(cPLA2), annexin 1 and COX-2 expression, and LPS-induced extracellular signal-regulated kinase (ERK) phosphorylation. Kanzo inhibited COX-2 activity but increased annexin 1 and COX-2 expression and did not alter LPS-induced ERK phosphorylation. Keihi inhibited COX-2 activity and LPS-induced ERK phosphorylation but slightly increased COX-2 expression and did not alter cPLA2and annexin 1 expression. These results suggest that the mechanism of shokyo is through the inhibition of cPLA2activity, and that of kanzo and keihi is through the inhibition of COX-2 activity and indirect inhibition of cPLA2activity. Therefore, it is possible that shokyo and kakkonto are clinically useful for the improvement of inflammatory responses.
Gas Classification Using Combined Features Based on a Discriminant Analysis for an Electronic Nose
Authors: Choi Sang-Il , Eom Taekyu , Jeong Gu-Min
Published: October 2016

This paper proposes a gas classification method for an electronic nose (e-nose) system, for which combined features that have been configured through discriminant analysis are used. First, each global feature is extracted from the entire measurement section of the data samples, while the same process is applied to the local features of the section that corresponds to the stabilization, exposure, and purge stages. The discriminative information amounts in the individual features are then measured based on the discriminant analysis, and the combined features are subsequently composed by selecting the features that have a large amount of discriminative information. Regarding a variety of volatile organic compound data, the results of the experiment show that, in a noisy environment, the proposed method exhibits classification performance that is relatively excellent compared to the other feature types.
Anti-D Antibodies in Pregnant D Variant Antigen Carriers Initially Typed as RhD+
Authors: Lukacevic Krstic Jelena , Dajak Slavica , Bingulac-Popovic Jasna , Dogic Vesna , Mratinovic-Mikulandra Jela
Published: October 2016

Background: To evaluate the incidence, the consequences, and the prevention strategy of anti-D alloimmunizations of D variant carriers in the obstetric population of Split-Dalmatia County, Croatia. Methods: RhD immunization events were evaluated retrospectively for the period between 1993 and 2012. Women were tested for RhD antigen and irregular antibodies. Those with anti-D antibody who were not serologically D- were genotyped for RHD. They were evaluated for their obstetric and transfusion history and their titer of anti-D. The neonates were evaluated for RhD status, direct antiglobulin test (DAT), hemoglobin and bilirubin levels, transfusion therapy as well as phototherapy and outcome. Results: Out of 104,884 live births 102,982 women were tested for RhD antigen. Anti-D immunization occurred in 184 women which accounts for 0.9% of individuals at risk of anti-D formation. 181 cases occurred in women serologically typed as D-. Three women were partial D carriers (DVa n = 2, DNB n = 1), initially typed RhD+, and recognized as D variant carriers after the immunization occurred. Anti-D titer varied from 1:1 to 1:16. Six children were RhD+, four had positive DAT, and two underwent phototherapy. Conclusion: Anti-D immunization occurred in pregnant partial D carriers (DVa, DNB). RhD+ children had serologic markers of hemolytic disease of the fetus and newborn (HDFN), with no cases of severe HDFN.
Diffuse Bullous Eruptions in an Elderly Woman: Late-Onset Bullous Systemic Lupus Erythematosus
Authors: Boddu Prajwal , Nadiri Mojtaba , Malik Owais
Published: October 2016

Vesiculobullous eruptions in the elderly represent a diverse range of varying pathophysiologies and can present a significant clinical dilemma to the diagnostician. Diagnosis requires a careful review of clinical history, attention to detail on physical and histomorphological examination, and appropriate immunofluorescence testing. We describe the case of a 73-year-old female who presented to our hospital with a painful blistering skin rash developed over 2 days. Examination of the skin was remarkable for numerous flaccid hemorrhagic bullae on a normal-appearing nonerythematous skin involving both the upper and lower extremities. Histopathology of the biopsy lesion showed interface change at the epidermo-dermal region with subepidermal blister formation, mild dermal fibrosis, and sparse interstitial neutrophilic infiltrate. Immunohistological analysis was significant for positive IgG basement membrane zone antibodies with a dermal pattern of localization on direct immunofluorescence and positive IgG antinuclear antibodies on indirect immunofluorescence. Evidence of antibodies to type VII collagen suggested the diagnosis of epidermolysis bullosa acquisita versus bullous systemic lupus erythematosus (BSLE). A diagnosis of BSLE was made based on positive American College of Rheumatology criteria, acquired vesiculo-bullous eruptions with compatible histopathological and immunofluorescence findings. This case illustrates one of many difficulties a physician encounters while arriving at a diagnosis from a myriad of immunobullous dermatoses. Also, it is important for internists and dermatologists alike to be aware of and differentiate this uncommon and nonspecific cutaneous SLE manifestation from a myriad of disorders presenting with vesiculobullous skin eruptions in the elderly.
Acute Kidney Injury Electronic Alert for Nephrologist: Reactive versus Proactive?
Authors: Kashani Kianoush , Ronco Claudio
Published: October 2016

Acute kidney injury (AKI) is a common complication of acute illnesses with significant impact on the mortality and morbidity. Early recognition of AKI allows clinicians to provide prophylactic interventions and to improve the outcomes of this deadly syndrome. Growing utilization and capabilities of electronic health records allow AKI risk stratification and early recognition with a potential effect on the processes of care and outcomes. We evaluate the current level of evidence on the impact of the AKI e-alert on the processes of care and outcomes. We then discuss the impacts of e-alerting in the design of the future of nephrology service within the hospital practices with suggestions regarding the role of nephrologists in management of AKI e-alert systems in order to improve the quality of care provided to AKI patients. Video Journal Club ‘Cappuccino with Claudio Ronco' at http://www.karger.com/?doi=450722.
Infecção pelo complexo Mycobacterium tuberculosis em carneiro da Barbária (Ammotragus lervia) no Zoológico de Curitiba, sul do Brasil: relato de caso
Authors: Morikawa Vivien Midori , Biondo Alexander Welker , Robes Rogério Ribeiro , Barros Filho Ivan Roque de , Zimpel Cristina Kraemer , Lara Maria do Carmo Custódio de Souza Hunold , Dib Cristina Corsi , Gequelin Luciana Cristina Fagundes , Paploski Igor Adolfo Dexheimer , Bonat Marcelo
Published: October 2016

A tuberculose é uma das doenças mundiais de notificação obrigatória mais importantes causada pelo complexo Mycobacterium tuberculosis que pode infectar pessoas e animais. A morte repentina de um carneiro da Barbária no Zoológico de Curitiba, que apresentou nódulos multifocais no pulmão à necropsia, levantou a suspeita de tuberculose. Foi realizada a Reação em Cadeia da Polimerase Quantitativa (qPCR) de fragmentos de órgãos e fluido. A qPCR detectou a presença do complexo M. tuberculosis nas amostras de pulmão. Este estudo relata a infecção pelo complexo M. tuberculosis no carneiro da Barbária, uma zoonose de grande relevância para a saúde pública, ressaltando-se a necessidade da implementação de medidas de prevenção. Além disso, pode prover um melhor entendimento sobre conservação de espécies, ocorrência e transmissão de doenças em cativeiro, potencial reservatório e impacto na saúde pública para visitantes e funcionários dos zoológicos.
Histological analysis of the eyeball of Neotropical birds of prey Caracara plancus, Falco sparverius, Rupornis magnirostris, Megascops choliba and Athene cunicularia
Authors: Pinto Débora Galdino , Cruz Guilherme Durante , Teixeira Rodrigo Hidalgo Friciello , Couto Erica Pereira , Carvalho Marcelo Pires Nogueira de
Published: October 2016

Nos últimos anos a oftalmologia aviária se tornou um instrumento de grande relevância para a conservação de aves de vida livre e de cativeiro, principalmente pela crescente utilização do exame oftalmológico para a triagem de indivíduos designados para reintrodução em ambientes naturais. Os olhos das aves de rapina são estruturas imprescindíveis para detectar e capturar suas presas, e a sua notável capacidade visual torna o seu sistema de visão assunto de grande interesse para o estudo anatômico e histológico. Neste contexto, no presente trabalho foi realizada a análise histológica do bulbo ocular de aves de rapina representadas por espécies pertencentes às ordens Falconiformes, Accipitriformes e Strigiformes presentes em território brasileiro. Para realização do estudo foram utilizados vinte bulbos oculares obtidos de animais mortos por causas naturais, das espécies Caracara plancus, Falco sparverius, Rupornis magnirostris, Megascops choliba e Athene cunicularia.. As lâminas histológicas foram coradas pela técnica de hematoxilina e eosina e, analisadas por microscopia óptica. Foi constatada a existência de um padrão histológico com discreta variação entre as estruturas oculares nas diferentes espécies avaliadas, com destaque para o cristalino e a retina. Este estudo salienta a importância da determinação do padrão histológico ocular das espécies, e seus resultados atribuem informações histológicas basais auxiliares na determinação de diagnósticos histopatológicos oculares.