bluuebunny/crossref_metadata_2025 huggingface dataset:
Direct Molecular Fishing of New Protein Partners for Human Thromboxane Synthase
Authors: Svirid A. V. , Ershov P. V. , Yablokov E. O. , Kaluzhskiy L. A. , Mezentsev Yu. V. , Florinskaya A. V. , Sushko T. A. , Strushkevich N. V. , Gilep A. A. , Usanov S. A. , Medvedev A. E. , Ivanov A. S.
Published: March 2020

Thromboxane synthase (TBXAS1) catalyzes the isomerization reaction of prostaglandin H2 producing thromboxane A2, the autocrine and paracrine factor in many cell types. A high activity and metastability by these arachidonic acid derivatives suggests the existence of supramolecular structures that are involved in the regulation of the biosynthesis and directed translocation of thromboxane to the receptor. The objective of this study was to identify TBXAS1 protein partners from human liver tissue lysate using a complex approach based on the direct molecular fishing technique, LC-MS/MS protein identification, and protein-protein interaction validation by surface plasmon resonance (SPR). As a result, 12 potential TBXAS1 protein partners were identified, including the components regulating cytoskeleton organization (BBIP1 and ANKMY1), components of the coagulation cascade of human blood (SERPINA1, SERPINA3, APOH, FGA, and FN1), and the enzyme involved in the metabolism of xenobiotics and endogenous bioregulators (CYP2E1). SPR validation on the Biacore 3000 biosensor confirmed the effectiveness of the interaction between CYP2E1 (the enzyme that converts prostaglandin H2 to 12-HHT/thromboxane A2 proantagonist) and TBXAS1 (Kd = (4.3 0.4) 10-7 M). Importantly, the TBXAS1CYP2E1 complex formation increases fivefold in the presence of isatin (indole-2,3-dione, a low-molecular nonpeptide endogenous bioregulator, a product of CYP2E1). These results suggest that the interaction between these hemoproteins is important in the regulation of the biosynthesis of eicosanoids.
A Highly Productive CHO Cell Line Secreting Human Blood Clotting Factor IX
Authors: Kovnir S. V. , Orlova N. A. , Shakhparonov M. I. , Skryabin K. G. , Gabibov A. G. , Vorobiev I. I.
Published: March 2020

Hemophilia B patients suffer from an inherited blood-clotting defect and require regular administration of blood-clotting factor IX replacement therapy. Recombinant human factor IX produced in cultured CHO cells is nearly identical to natural, plasma-derived factor IX and is widely used in clinical practice. Development of a biosimilar recombinant human factor IX for medical applications requires the generation of a clonal cell line with the highest specific productivity possible and a high level of specific procoagulant activity of the secreted factor IX. We previously developed plasmid vectors, p1.1 and p1.2, based on the untranslated regions of the translation elongation factor 1 alpha gene from Chinese hamster. These vectors allow one to perform the methotrexate- driven amplification of the genome-integrated target genes and co-transfect auxiliary genes linked to various resistance markers. The natural open reading frame region of the factor IX gene was cloned in the p1.1 vector plasmid and transfected to CHO DG44 cells. Three consecutive amplification rounds and subsequent cell cloning yielded a producer cell line with a specific productivity of 10.7 0.4 pg/cell/day. The procoagulant activity of the secreted factor IX was restored nearly completely by co-transfection of the producer cells by p1.2 plasmids bearing genes of the soluble truncated variant of human PACE/furin signal protease and vitamin K oxidoreductase from Chinese hamster. The resulting clonal cell line 3B12-86 was able to secrete factor IX in a protein-free medium up to a 6 IU/ml titer under plain batch culturing conditions. The copy number of the genome- integrated factor IX gene for the 3B12-86 cell line was only 20 copies/genome; the copy numbers of the genome-integrated genes of PACE/furin and vitamin K oxidoreductase were 3 and 2 copies/genome, respectively. Factor IX protein secreted by the 3B12-86 cell line was purified by three consecutive chromatography rounds to a specific activity of up to 230 IU/mg, with the overall yield 30%. The developed clonal producer cell line and the purification process employed in this work allow for economically sound industrial-scale production of biosimilar factor IX for hemophilia B therapy.
High-Throughput Screening of Biodiversity for Antibiotic Discovery
Authors: Terekhov S. S. , Osterman I. A. , Smirnov I. V.
Published: March 2020

The increasing number of infections caused by antibiotic-resistant strains of pathogens challenges modern technologies of drug discovery. Combinatorial chemistry approaches are based on chemical libraries. They enable the creation of high-affinity low-molecular-weight ligands of the therapeutically significant molecular targets of human cells, thus opening an avenue toward a directed design of highly effective therapeutic agents. Nevertheless, these approaches face insurmountable difficulties in antibiotic discovery. Natural compounds that have evolved for such important characteristics as broad specificity and efficiency are a good alternative to chemical libraries. However, unrestricted use of natural antibiotics and their analogues leads to avalanche-like spread of resistance among bacteria. The search for new natural antibiotics, in its turn, is extremely complicated nowadays by the problem of antibiotic rediscovery. This calls for the application of alternative high-throughput platforms for antibiotic activity screening, cultivation of unculturable microorganisms, exploration of novel antibiotic biosynthetic gene clusters, as well as their activation and heterologous expression. Microfluidic technologies for the screening of antibiotic activity at the level of single cells are, therefore, of great interest, since they enable the use of a single platform to combine the technology of ultrahigh-throughput screening, next-generation sequencing, and genome mining, thus opening up unique opportunities for antibiotic discovery.
Antidepressant Effect of an Orally Administered Dipeptide Mimetic of the Brain-Derived Neurotrophic Factor
Authors: Povarnina P. Yu. , Garibova T. L. , Gudasheva T. A. , Seredenin S. B.
Published: March 2020

Involvement of BDNF in the regulation of neuroplasticity and neurogenesis in the hippocampus, impairment of which underlies the pathophysiology of depression, makes this endogenous protein a promising object for the development of new-generation antidepressants with a neurophysiologically based mechanism of action. A low-molecular-weight BDNF mimetic, GSB-106 (a substituted dimeric dipeptide, bis-(N-monosuccinyl- L-seryl-L-lysine) hexamethylenediamide), was designed and synthesized at the Zakusov Institute of Pharmacology. GSB-106 was found to activate BDNF-specific TrkB receptors and their main post-receptor signaling pathways MAPK/ERK and PI3K/AKT. GSB-106 exhibited pronounced antidepressant activity in a rodent test battery at a dose of 0.1 to 1.0 mg/kg administered intraperitoneally. Because oral administration is preferable in the treatment of depression, which is associated with a prolonged duration and outpatient character of pharmacotherapy, we examined the antidepressant properties of GSB-106 administered orally as a pharmaceutical substance (PS) and in tablet dosage form (TDF). In the study, we used the Porsolt forced swim test in rats; a conventional antidepressant, Amitriptyline, was used as a reference drug. The antidepressant activity of GSB-106 was found to retain upon oral administration and to manifest at doses of 0.5-5.0 mg/kg for PS and 0.01-5.0 mg/kg for TDF. The effective dose of TDF was 50-fold lower than that of PS, and the efficacy of tableted GSB-106 exceeded that of Amitriptyline, the gold standard in antidepression care. Therefore, GSB-106, both as a substance and as a tablet dosage form, exhibits antidepressant activity when administered orally, which makes it a promising antidepressant agent, the first in the class of BDNF mimetics.
Zinc Finger Protein CG9890 - New Component of ENY2-Containing Complexes of Drosophila
Authors: Fursova N. A. , Nikolenko J. V. , Soshnikova N. V. , Mazina M. Y. , Vorobyova N. E. , Krasnov A. N.
Published: March 2020

In previous studies, we showed that the insulator protein Su(Hw) containing zinc finger domains interacts with the ENY2 protein and recruits the ENY2-containing complexes on Su(Hw)-dependent insulators, participating in the regulation of transcription and in the positioning of replication origins. Here, we found interaction between ENY2 and CG9890 protein, which also contains zinc finger domains. The interaction between ENY2 and CG9890 was confirmed. It was established that CG9890 protein is localized in the nucleus and interacts with the SAGA, ORC, dSWI/SNF, TFIID, and THO protein complexes.
Assessment of the Phenylketonuria (PKU)-Associated Mutation p.R155H Biochemical Manifestations by Mass Spectrometry-Based Blood Metabolite Profiling
Authors: Baturina O. A. , Chernonosov A. A. , Koval V. V. , Morozov I. V.
Published: March 2020

Homozygous siblings with different treatment histories represent an excellent model to study both the phenotypic manifestation of mutations and the efficacy of therapy. We compared phenylketonuria (PKU) manifestations in two different gender siblings who were homozygous carriers of a rare phenylalanine hydroxylase (PAH) mutation, p.R155H, subjected to different treatments. PKU caused by mild mutations may be easily underdiagnosed if the diagnosis is based solely on the phenylalanine (Phe) blood concentration. One of the described patients is an example of this diagnostic error. For reducing diagnostic errors, we suggest the use of more elaborate methods in screening practice, in particular mass spectrometric analysis of blood metabolites, the efficiency of which is demonstrated in the present study.
PREDIKSI MODEL FINANCIAL DISTRESS (KEBANGKRUTAN) PADA PERUSAHAAN RITEL BURSA EFEK DENGAN MODEL ALTMAN DAN SPRINGATE INDONESIA PERIODE 2012-2016
Authors: Barry Husnil
Published: March 2020

ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi model kebangkuratn yang paling powerfulluntuk perusahaan ritel di Indonesia dan memprediksi kondisi perusahaan yang akan mengalamikebangkrutan masa yang akan datang. Model yang digunakan dalam penelitian ini yaitu Altman ZScoredan Springate. Hasil dari model tersebut adalah dapat diketahui kondisi-kondisi perusahaantersebut sehat atau diambang kebangkrutan. Data yang digunakan dalam penelitian ini berjumlah 25perusahaan yang termasuk dalam industri ritel yang sudah listing di Bursa Efek Indonesia tahun2012-2016.  Kata kunci: Altman, Springate, Financial Distress. ABSTRACT This study aims to identify the most powerful disaster models for retail companies inIndonesia and to predict the condition of companies that will experience bankruptcy in the future. Themodel used in this study is Altman Z-Score and Springate. The results of the model are the conditionsof the company, the health or whether they are on the verge of bankruptcy are identified. The dataused in this was 25 companies retail industry that had been listed on the Indonesia Stock Exchange in2012-2016. Kata kunci: Altman, Springate, Financial Distress.
PENGARUH CORPORATE GOVERNANCE TERHADAP NILAI PERUSAHAAN DI BURSA EFEK INDONESIA STUDI PADA SEKTOR CONSUMER GOODS PERIODE 2013-2017
Authors: Pramesti Tasya Anindya , Mirati Elly , Kuncoro Petrus Hari
Published: March 2020

PENGARUH CORPORATE GOVERNANCE TERHADAP NILAI PERUSAHAAN DIBURSA EFEK INDONESIA STUDI PADA SEKTOR CONSUMER GOODS PERIODE 2013-2017Tasya Anindya Pramestitasya_pramesti@outlook.comR. Elly MiratiEllymirati@yahoo.co.idPetrus Hari Kuncoro Senohariseno@gmail.comProgram Studi Manajemen Keuangan Politeknik Negeri JakartaABSTRACTCorporate governance is one of the most important mechanism in the internal companymanagement to keep the company performance with the intention to incrase the firm value. Thepurpose of this study is to test the influence of corporate governance on firm value which is proxiedwith PBV. This research is quantitative and assocative research where data is obtained from annualreport from company that listed in Indonesia Stock Exchange in the consumer goods sector in 2013-2017 published by BEI. The population of this study are 46 companies with the sampling technique used is purposive sampling and 6 companies are choosen. The data analysis method used is panel regression through several tests including classic assumption test and hyppothesis testing partially and simultaneously. The results of this study are managerial ownership is significantly influenced firm value, but institutional ownership, independent commisioner, and audit committee has no influence on firm value significantly, and corporate governance simultaneously is significantly influenced firm value.Keywords: Corporate Governance, Firm Value, PBV ABSTRAKCorporate governance merupakan salah satu mekanisme yang sangat penting dalam suatuinternal manajemen perusahaan untuk menjaga kinerja perusahaan dengan tujuan meningkatkannilai perusahaan. Tujuan dari penelitian ini adalah untuk menguji pengaruh corporate governanceterhadap nilai perusahaan yang diwakilkan dengan PBV. Penelitian ini adalah penelitian kuantitatifdan asosiatif dimana data diperoleh dari laporan tahunan perusahaan yang terdaftar di Bursa EfekIndonesia sektor consumer goods pada periode 2013-2017 yang diterbitkan oleh BEI. Populasi daripenelitian adalah 46 perusahaan dengan teknik pengambilan sampel menggunakan purposivesampling dan hasil sampel penelitian ini sebanyak 6 perusahaan. Metode analisis data yangdigunakan adalah metode regresi data panel termasuk pengujian asumsi dan kesesuaian model danpengujian hipotesis baik secara parsial dan secara simultan. Hasil dari penelitian ini menunjukanbahwa kepemilikan manajerial berpengaruh terhadap nilai perusahaan, sedangkan kepemilikaninstitusonal, komisaris independen, dan komite audit tidak berpengaruh signifikan terhadap nilaiperusahaan, dan corporate governance secara simultan berpengaruh terhadap nilai perusahaan. Kata kunci: Corporate Governance, Nilai perusahaan, PBV
Peningkatan Keterampilan Berbicara Bahasa Inggris Melalui Media Film
Authors: Hanafiah Wardah
Published: March 2020

Penelitian ini bertujuan untuk mengetahui proses dan hasil peningkatan keterampilan berbicara bahasa Inggris menggunakan media belajar film. Film sebagai media belajar sangat disukai oleh mahasiswa sehingga memudahkan mahasiswa berinteraksi. Metode penelitian menggunakan penelitian tindakan. Proses pengumpulan data adalah observasi, catatan harian, dokumentasi dan tes. Proses validasi data penelitian dilakukan selama proses kegiatan tindakan dan hasil yang diperoleh serta melibatkan kolaborator. Hasil penelitian menunjukkan media belajar film memberikan dampak yang positif terhadap peningkatan keterampilan berbicara bahasa Inggris. Hal ini disebabkan karena penerapan media belajar film menjadi sangat menyenangkan. Nilai rata-rata dari siklus pertama ke siklus kedua sampai siklus ketiga mengalami peningkatan yang cukup signifikan. Nilai rata-rata siklus 1 adalah 62,2. Nilai rata-rata siklus 2 adalah 71,2. Nilai rata-rata siklus 3 ada;ah 80,5.
EFISIENSI DENGAN KONVERSI SATUAN HARGA TINTA RP/GRAM MENJADI RP/SHEET PADA TINTA FLEKSO
Authors: Yuniarti Endang , Putri Risya Kusna
Published: March 2020

There agreement between ink companies and printing companies that is the ink company will supply printing ink according to the needs of the printing company and if there is excess ink, it will be requested. Ink returns according to company data reach 50 to 80%. The problem that arises is the problem of ink that will become junk, and the impact is inefficiency. The purpose of this study is to test the selling price unit of RP/KG ink to become RP/Sheet. The method used in this study is the calculation of BM area using Automatic Cad and is calculated using ink in gr/m2 units which is then used to be gr/sheet units and converted again to the unit price, which is Rp/sheet. The object of research is KKG instant noodles. This research resulted in the conversion of black and white price units used in this instant noodle product with units of Rp/gr indicating a more expensive price of Rp.49,806 per gram while with units of Rp/sheet of Rp17,34 per sheet. So by using units of Rp/sheet, give the ink company to estimate when sending ink so that it can be minimized from printing companies.
MODIFIKASI BUCKET ELEVATOR 536 PADA PT X
Authors: Widiawaty Candra Damis , Agil Averoes Syahputra , Hangesthi Bayu Chandra
Published: March 2020

In the production process at PT X, such as casting process, required raw material in the form of casting sand. The sand transport process is done by a machine called a Bucket Elevator. The current condition of Bucket Elevator at Plant 2 PT X had some problems on its design due to the initial modification process to the machine that were deliberately done in order to ease the process of troubleshooting and repair on the machine to adjust the height of the drum shaft supported by the bearing, however, the modification causes a sand leak that impacted the lifetime of the bearing. The damage that occurs to the bearing of the Bucket Elevator can slow the production process at PT X down, which lasts between 120 hours to 134 hours per week. Therefore, it is necessary to modify the design of the Bucket Elevator machine in order to stop the sand leakage and be able to work optimally. The design modifications made, are changes to the shaft height adjusting mechanism of the Bucket Elevator 536 drum machine by providing a lock to minimize the leakage of sand that occurs and replace the bearing type to the type that is equipped with a seal to prevent the entry of sand into the bearings. The result of this modification can extend the lifetime bearing, thereby reducing production costs due to bearing replacement, and easing the troubleshooting and repairing process of the engine to adjust the height of the drum shaft supported by the bearings.
Host Country Brand Image and Political Consumerism: The Case of Russia 2018 FIFA World Cup
Authors: Rocha Claudio , Wyse Fiona
Published: March 2020

Abstract: The aim of this study was to investigate whether 2018 FIFA World Cup (FWC) consumers would engage in political consumerism to reduce a perceived dissonance between host country (Russia) brand image and FWC brand attributes. Literature has associated Russia-hosted sport mega-events (SME) with violations of human rights. Drawing on cognitive dissonance theory, we investigate UK consumers ( n = 417) on their perceptions about Russia’s brand image (cognitive and affective attributes) and FWC brand attributes before and after the event. Online questionnaires were used to collect data, which were analyzed via covariance-based structural equation modeling. Findings showed that intentions did not differ from behaviors of political consumerism toward 2018 FWC products. Lower evaluations of affective host image attributes led to more political consumerism. Higher perceptions of the FWC attributes led to less political consumerism. Results inform FIFA (and possibly other SME owners) on how poor human rights practices (affective attributes) may affect the consumption of their products. Results also inform hosts about ineffectiveness of associating themselves with brands like FWC without showing concerns about human rights.
Entrepreneurial Performance Improvement with Organizational Creativity and Entrepreneurial Orientation
Authors: Natia Afriany Arista , Prabowo Muhhamad Agung , Yulianto Alfian , Setyo Budiarto Dekeng
Published: March 2020

SMEs (Small and Medium-sized Enterprises) have a significant role in developing countries, especially in Indonesia. In Indonesia, SMEs can absorb a lot of workforces, thereby reducing unemployment. This study aims to examine the effect of organizational creativity, artistic orientation, entrepreneurial orientation, and financial literacy on entrepreneurial performance. The research object is creative MSMEs in Yogyakarta. The respondents of this study were 139 creative SME’s owners or managers. This research uses multiple linear regression analysis to prove the hypothesis. This research is the development of some previous findings by testing the effect of organizational creativity with the entrepreneurial performance that has never been studied before. The results showed there was an influence between organizational creativity, entrepreneurial orientation on entrepreneurial performance. This research has implications for SME owners to improve creativity if they want to achieve optimal entrepreneurial performance.
Barriers to End-of-life Discussions in the Primary Care Setting
Authors: Devon S. Boydstun, DO  , Shandra Basil, OMS-IV  , Jill Porter, DO  , Anand Gupta, MBBS, MPH
Published: March 2020

Background: The Patient Self Determination Act was passed in 1991 and requires healthcare facilities to present patients with information regarding advanced directives. Since that time, there has been no improvement in the number of patients reported to have had such discussions. Numerous barriers to these discussions exist both on the patient and provider side. This study aims to identify barriers to end of life discussions among providers in the primary care setting.

Methods: The study population included practicing primary care physicians in the OhioHealth system. They were administered an anonymous questionnaire addressing demographic information and questions specific to end of life discussions and what barriers exist.

Results: A majority of primary care physicians reported engaging in end of life discussions with their patients. A majority of physicians cited lack of time as a barrier to having these discussions. There was a statistically significant age difference among primary care physicians who reported they have end of life discussions with their patients and among these physicians there was a statistically significant increase in their level of comfort having these discussions.

Conclusion: Primary care physicians further into their career reported having end of life discussions more frequently and felt more comfortable doing so. Additionally, physicians cite lack of time as the most common barrier to holding end of life discussions.
Triple Helix Synergy for Development and Enhancing Competitiveness of SMEs
Authors: Herdiana Dyah Susanti  , Dian Arief Pradana  , Robit Nurul Jamil
Published: March 2020

Micro, small, and medium enterprises (SMEs) is the work done by individuals in the form of economic enterprises that meet the criteria of SMEs as set out in(Act No. 20 of 2008, 2008)about SMEs. The human factor is the most important factor in the development of SMEs to improve product quality and improve the welfare of the community. The triple helix is an innovative model of students, researchers, and policymakers to improve the university's role in improving the economic and social development, improve the role of governments to develop innovation, enhance enterprise collaboration with governments and universities to boost innovation. This study aims to (1) show the relationship triple helix in the development of the competitiveness of SMEs. (2) The effectiveness of the application of triple helix in the development of the competitiveness of SMEs. This study is quasi experimental research with pretest-posttest results of the application of triple helix on SMEs in Banyuwangi.
PENGARUH KOMBINASI PUPUK KANDANG DENGANUREA TERHADAP PERTUMBUHAN SAWI (Brassica juncea L.)
Authors: Yuliani Ida , Utami Septiana Dwi , Efendi Ismail
Published: March 2020

This study aims to determine the effect of combination of manure with urea on mustard greens. This type of research is true experiment. The experiment was conducted in experimental garden located in Pejeruk Bangket neighborhood of Pejeruk Village, Ampenan District, Mataram City. The research was using Completely Randomized Design (RAL) with 4 treatment of 6 replications with combination of manure with different urea on mustard plant P0 (Control), P1 (100% manure), P2 (50% manure + 50% urea fertilizer) P3 (100% urea fertilizer). The data of the research were analyzed using Analysis of Variance and continued by Duncan test if there was effect of cow manure komninasi treatment with urea to growth of mustard greens. The results showed that the combination of manure with urea on the growth of the mustard greens had a significant effect on plant height and wet weight, while the leaf length and leaf width did not significantly affect. The effect of fertilizer combination shows different results on each parameter. In the highest plant growth parameter height at P2; Leaf length at P1; leaf width at P1; and wet weight on P2.
PENERAPAN LKS BIOLOGI BERBASIS EKSPERIMEN UNTUK MENINGKATKAN KEMAMPUAN ILMIAH SISWA
Authors: Rohani Rohani , Muliadi Agus , Nurhidayati Siti
Published: March 2020

LKS which has been applied in schools has made students less developed scientific abilities. Worksheets that are used seem monotonous, use a colorless image, so it looks less attractive. Student worksheets are only given to be done by students, without having to guide students in solving problems contained in worksheets, and the worksheet does not contain what students have to do such as observing, analyzing, synthesizing, solving problems because there is no guidance to conduct experiments and scientific attitudes . So that makes students less able to think scientifically in learning, for that applied Experiment Based Biology Worksheet where Experiment Based Biology Worksheet can increase student activity. The purpose of this study is to apply biological worksheets to improve students' scientific abilities. This research includes Classroom Action Research (CAR) which consists of 2 cycles, each cycle has 4 stages including; planning, implementing, observing, evaluating, and reflecting. The instruments in this study were the learning implementation observation sheet (RPP) and the scientific ability test sheet. Research data were analyzed descriptively. The results of the study showed that the results of the implementation of the Learning Implementation Plan (RPP) in cycle 1 reached 75% in the good category and in cycle 2 it reached 90% in the very good category, while the students' scientific ability data in the first cycle reached 49.06 with the less scientific category , increased in second cycle reached 79.57 with the scientific category. The results of the study concluded that the application of experiment-based worksheet could improve students' scientific abilities.
PENGARUH MODEL PEMBELAJARAN CPS (CREATIVE PROBLEM SOLVING) TERHADAP KETERAMPILAN BERPIKIR KREATIF DAN HASIL BELAJAR KOGNITIF SISWA
Authors: Munisah Munisah , Nurhidayati Siti , Royani Ida
Published: March 2020

The problem that happened in MTs NW Darussholihin Wassyakirin is creative thinking skill and cognitive learning result of student is still less satisfactory, proved by the value of MID semester under KKM (Criteria Completed Minimal). This is because the learning process is not effective because the use of methods or learning models that are not effective. The purpose of this research is to know the influence of CPS (Creative Problem Solving) model of creative thinking skill and students' cognitive learning outcomes. This type of research is quasi experimental research with pretest postest control group design research design. The instrument used in this research is the observation sheet of learning implementation (RPP), test of creative thinking skill and test of cognitive learning result of the students. Data collection techniques are observations and tests, while data analysis techniques are descriptive and t-test. The results showed that the data presentation of creative thinking skills of students in the experimental class is 85.33 with very high category while the result of cognitive learning posstest students get the average value in the experimental class that is 80.83 and the control class is 78.67. The result of study posttest showed that the average learning result of the students in the experimental class and the control class were 86 and 76. Based on the t-test analysis with separated variance formula with 5% significant, student learning result obtained tcount of 2,581 and ttable value of 2.048, means tcount is greater than ttable value (2.581 > 2.048) so that (Ho) is rejected (Ha) accepted. Based on these results can be concluded that the model of learning CPS (Creative Problem Solving) effect on student cognitive learning outcomes, and average creative thinking skills achieve high categories.
UJI PENDAHULUAN EFEKTIVITAS BIOINSEKTISIDA AKAR TUBA TERHADAP HAMA Oxya chinensis PADA SKALA LABORATORIUM
Authors: Yama Danie Indra , Soesatrijo Jojon , Santiko Rusnadi
Published: March 2020

The aim of this research is to knowtime of death, mortality and changes in behavior of Oxya chinensis in the treatment of botanical insecticides root of the tuba. The research was conducted at Laboratory Citra Widya Edukasi Palm Oil Polytechnic in Bekasi, the experimental design was the Completely Randomized Design, the treatment consisted of A0 = deltamethrin 0.3 cc/100 ml, A1 = tuba root extract 3g/100ml, A2 = tuba root extract 4g/100ml , A3 = tuba root extract 5g/100ml. Each treatment was repeated three times and each replication contained of 3 samples. Data were analyzed using variance with 5% level, if there is a real effect then tested further with the test of the Least Significance Different. Making extracts by means of tuba roots chopped until smooth, mixed with 100 ml of water, saved for 3 days, then filtered and taken water and the application is sprayed. Pest control using chemical insecticides is more effective than botanical insecticides root of the tubabut 4g/100ml botanical insecticides toba root are also potentially in Oxya chinensis death at 39,51 minutes after the application. The application of chemical and botanical insecticides made from tubaroots causes Oxya chinensis to decrease activity than dies.
ANALISIS MISKONSEPSI SISWA DALAM MENYELESAIKAN SOAL MISKONSEPSI PADA MATA PELAJARAN BIOLOGI
Authors: Hajiriah Titi Laily , Mursali Saidil , Dharmawibawa Iwan Doddy
Published: March 2020

Biology is a basic science for studying living things. Basically, some biological content that is contemporary in the learning process is not only in the form of transfer of information and facts from the teacher to students through what they see and what they hear through specific information processing, but requires literal understanding of concepts. Provision of material biological concepts become biased and inherent in one's understanding and cause cognitive conflict that leads to misconceptions. The purpose of this study is to describe the results of the analysis of students' mastery of concepts in solving problems that are considered correct but theoretically wrong. The analysis used in this study was by identifying students' level of understanding in a material and concept. Conclusions from the study that the level of student misconception through its CRI analysis> 2.5 of 30 students, 97% experienced misconception. For this reason, it is necessary to study biological material and be revised by the teacher and students by studying the material and concepts that are correct or bringing in experts in the field of biology.
IMPLEMENTASI PERANGKAT PEMBELAJARAN BIOLOGI BERORIENTASI INQUIRY UNTUK MENINGKATKAN KREATIVITAS SISWA
Authors: Masiah Masiah
Published: March 2020

The purpose of this study is to see the practicality and effectiveness of the learning tools that have been developed so as to enhance students' creative ideas. This type of research is pre-experimental research design with one group pre-test village post-test. The instrument in this study was the observation sheet of the implementation of learning, student questionnaire responses and tests of creative thinking skills. The data obtained were analyzed descriptively quantitatively. The results showed that students' responses to the learning process by using inquiry-oriented learning tools in general students felt interested and the results of thinking ability tests showed a classical completion. Based on these results it can be concluded that the implementation of inquiry-oriented learning tools can be declared practical and effective to improve students' creative ideas.
PENGARUH METODE EKSTRAKSI KENCUR (Kaempferia galanga L.) TERHADAP PERTUMBUHAN Staphylococcus aureus
Authors: Primawati Sri Nopita , Jannah Husnul
Published: March 2020

Kaempferia galanga L. or known as Kencur is an herbal plant that is widely used by people with various benefits such as influenza in infants, headaches, sprains, relieving fatigue, inflammation of the stomach, coughing, improving menstruation, inflammation of children's ears, dirty blood, eyes aches, diarrhea, and colds. However, studies that have used Kencur as an antibacterial against Staphylococcus aureus have never been done. Therefore, scientific research is needed to determine the ability of Kencur extract as an antibacterial that inhibits the growth of Staphylococcus aureus. 3 extraction methods were used, with the aim of determining the right method for obtaining Kencur bioactive compounds as antibacterial. The three extraction methods used were the press method, the infusion method, and the maceration method using methanol as a solvent. Each extract obtained from various extraction methods will be tested for antibacterial by observing the growth of Staphylococcus aureus. It was found that with the press method there were no clear zones or growth inhibition zones of Staphylococcus aureus. Using the extract from the infusion method, the same thing is obtained, namely there is no clear zone or growth inhibition zone of Staphylococcus aureus. However, it is different when given an extract from the maceration method using methanol solvent, a 17 mm clear zone is formed but there is no inhibition zone. This can indicate that, methanol extract from Kencur can inhibit the growth of Staphylococcus aureus (17 mm).
Penukaran Delivery Order Online dan E-Container Equipment Interchange Receipt Terhadap Impor Barang Menggunakan Petikemas
Authors: Prastyorini Juli , Syaputra Fahreza Achmad
Published: March 2020

Penelitian ini bertujuan untuk mendeskripsikan tentang penanganan barang impor menggunakan petikemas dengan menggunakan delivery order online dan elektronik container equipment interchange receipt. Dalam penelitian metode yang digunakan ini ialah metode deskriptif. Sumber data yang digunakan dalam penelitian ini adalah wawancara dan pengamatan langsung di lapangan. Hasil penelitian ini memperlihatkan proses penanganan impor barang menggunakan bill of lading original yang ditukar dengan delivery order online yang masih menggunakan sistem manual di perusahaan pelayaran dan di job order-kan menjadi elektronik container equipment interchange receipt menggunakan aplikasi clique-247 untuk petikemas keluar dari pelabuhan sandar guna mempercepat penanganan impor barang menggunakan petikemas bagi pengguna jasa dan pemberi pelayanan jasa untuk bekerja dengan praktis dan cepat. Berdasarkan hasil wawancara, pengamatan, studi dokumentasi dan studi pustaka dalam penelitian, dapat diperoleh kesimpulan bahwa delivery order online dan elektronik – container equipment interchange receipt yang dikerjakan oleh operasional Ekspedisi Muatan kapal Laut dapat mengeluarkan petikemas dalam pelabuhan sandar kapal. Artinya, model konseptual yang dikembangkan dan dilandasi oleh teori hampir sepenuhnya didukung oleh fakta yang ada dilapangan.
Peran Crude Oil Washing System (COW) Pada Kapal Tanker
Authors: Sigit Prasetya
Published: March 2020

Crude Oil Washing System adalah sistim yang  menggunakan Crude Oil atau minyak mentah sebagai  medium pencuci. Tanki-tanki muatan diusahakan bersih dari sisa-sisa muatan minyak mentah sehingga balas bersih dapat dimuat dalam tanki yang  sudah dibilas tadi dengan  hanya mengadakan sedikit pembilasan lebih dahulu dengan  air sehingga  akibat pollusi akan  sangat  terbatas. Pipa yang  digunakan yang  dihubungkan dengan  pipa muatan harus dibuat dari pipa baja atau sejenisnya yang  kekuatannya cukup untuk  menahan tekanan minyak yang  diperlukan, dan juga harus terpasang (fixed) dan diperkuat dengan  baik. Crude Oil Washing System harus dipasang permanent dan terpisah sama sekali dari system pipa-pipa yang  lain kecuali yang  disambung kepipa muatan. Juga pipa-pipa tersebut  harus dilengkapi dengan  relief system guna mengontrol tekanan dalam pipa jangan sampai berlebih, relief pipe system ini harus dihubungkan kembali dengan  bagian isap dari Supply Pump. Sedangkan keuntungan menggunakan Crude Oil adalah sudah tersedia dikapal dengan  cara resirkulasi, efektif untuk  menghilangkan sediment dan sisa-sisa minyak yang  melekat dalam  tanki (good Carrier) dan dibongkar bersama-sama dengan  muatan, tidak menimbulkan karat serta tidak ada kemungkinan emulsi air dan minyak jadi mengurangi bahaya pollusi. Semua pekerjaan Crude Oil Washing  harus sudah selesai sebelum kapal meninggalkan pelabuhan bongkar muatan terakhir.Jika sampai dilaksanakan Crude Oil Washing antara dua pelabuhan bongkar pada tanki-tanki tersebut  harus tetap kosong untuk  pemeriksaan pada pelabuhan-pelabuhan berikutnya sebelum diisi dengan  air balas.
Elementary pre-service teachers working in mathematical borders
Authors: López Leiva Carlos Alfonso
Published: March 2020

Using the border metaphor to portray the common separation between school mathematics and students’ everyday lives, this manuscript describes the processes that a group of elementary pre-service teachers (PSTs) have experienced to learn about their students and bridge these borders. Content analysis of mathematical activities and PSTs' reflections developed in a mathematics teaching methods course reveals that although they asserted that the process of developing bridges between mathematics and everyday experiences has transformed their self-concept and confidence in mathematics and teaching, they mostly use their own knowledge and experience to develop these tasks rather than from their students’. Possibilities to generate more genuine ethnomathematical approaches are discussed.
IMPLEMENTASI PROGRAM KEMITRAAN & BINA LINGKUNGAN STUDI KASUS DI PT ANGKASA PURA II (PERSERO) CABANG UTAMA BANDAR UDARA SOEKARNO-HATTA
Authors: Hasanuddin Mohamad
Published: March 2020

PT Angkasa Pura II  (Persero) adalah Perurahaan Badan Usaha Milik Negara ( BUMN) yang mengelola Airport di Indonesia yang menerapkan  program CSRSeperti telah disebutkan dalam peraturan Menteri Badan Usaha Milik Negara bahwa ada dua kegiatan yang harus dilakukan oleh PT Angkasa Pura II (Persero) yaitu Program kemitraan dengan usaha kecil dan menengah dan juga Program Pengembangan masyarakat  atau yang biasa disebut Program kemitraan dan Bina Lingkungan (PKBL)Tujuan dari penelitian ini adalah untuk mengetahui kendala dalam pelaksanaan Program Kemitraan dan Bina Lingkungan di BUMN PT Angkasa Pura II (Persero) dan untuk mengetahui peaksanaan Program Kemitraan dan Bina Lingkungan  Pada Badan Usaha Milik Negara PT Angkasa Pura II (Persero) Kantor  Cabang  Utama Bandara Soekarno-Hatta  yang telah dilaksanakan sesuai dengan UU tentang BUMN  dan peraturan Menteri BUMN Nomor: PER-02 / MBU / 7/2017.Penelitian ini  menggunakan hukum empiris yaitu literatur penelitian yang tersedia, yaitu penelitian data primer. Data yang diperoleh dalam literatur penelitian tersedia dan wawancara sedang diproses dan dianalisis secara kualitatif normatif makna analisis data berdasarkan apa yang diperoleh dari literatur yang tersedia dan wawancara kemudian diarahkan, dibahas dan diberi penjelasan dengan ketentuan yang berlaku, dan akhirnya disimpulkan dengan metode induktif, yaitu menarik kesimpulan dari hal ke hal khusus yang umum.Kendala dalam pelaksanaan program kemitraan dan Bina Lingkungan  PT Angkasa Pura II (Persero)  Kantor Cabang Utama  Bandara Soekarno-Hatta sejak didirikan pada tahun 1985 adalah  memiliki masalah klasik yaitu kebisingan bagi masyarakat  sehingga perlu untuk mencoba mengendalikan untuk menguranginya, salah satunya adalah membangun tembok untuk mengurangi (noise barrier) dan memindahkan permukiman yang sangat dekat dengan landasan pacu.
A Study on Uniaxial Tensile Deformation Behavior of Superelastic Titanium Alloy
Authors: Jeong Hye-Jin , Luu Viet Tien , Jeong Yong-Ha , Hong Sung-Tae , Han Heung Nam
Published: March 2020

A superelastic titanium alloy was subjected to uniaxial tensile deformation at room temperature. The microstructural evolution and deformation mechanisms of the superelastic titanium alloy were investigated by electron backscatter diffraction (EBSD) and X-ray diffraction (XRD). Multiple deformation mechanisms including stress-induced martensitic transformation (SIMT), dislocation slip, {332}<113> and {112}<111> mechanical twinning were identified with the increase in uniaxial strain. In the early stage of deformation, a SIMT from the bcc beta phase to orthorhombic martensite phase dominantly occurred. As the deformation proceeded, the phase fraction of the remained martensite which did not return to beta phase obviously increased due to dislocation slip and mechanical twinning. The kernel average misorientation (KAM) value obtained from EBSD data gradually increased with increasing the deformation, indicating that the dislocation evolution was produced by slip. This was well matched with the trend in the full width at half maximum (FWHM) value of the peak profile obtained from XRD data. In addition, the fraction of the {332}<113> twin was lower than that of the {112}<111> twin in the initial specimen. However, the {332}<113> twin rapidly increased compared to the {112}<111> twin as deformation increased. Therefore, it is confirmed that {332}<113> twinning and dislocation slip were the dominant mechanisms during plastic deformation.
Effect of a Metal Thin Film's Residual Stress and Manufacturing Process on Thin Film Micro-Crack
Authors: Cho Byung-Kyu , Hong Seung-Chan , Kim Byung-Sam , Cheon Jae-Kyung
Published: March 2020

In recent automobile trends, the functions of opening and closing the vehicle door are accomplished by touch sensor and smart-phone NFC (Near Field Communication) systems. These convenience features are incorporated into the outdoor handle. However, this function can’t be used when chrome plating is applied to this part for design purposes. To solve the problem of chrome plating, we studied a metal sputtering deposition process technology, which can preserve the metal feeling without interfering with NFC and touch sensor operation. To achieve this interface communication and sensing performance, we developed a surface treatment that can generate micro-cracks in the thin film layer. We also investigated how the door handle manufacturing process affected the shape of the micro-cracks in the thin film. Results showed that the thickness of the thin film and the target power played a crucial role in controlling the residual tensile stress in the thin film, which was one of major factors responsible for generating micro-cracks in the thin film layer. The shape of the micro-cracks in the thin film was affected by the adjacent layers of the thin film, the primer paint and UV top coat. The surface energy of the primer paint and the shear stress produced by the hardening of the UV top coat were found to affect the shape of the micro-cracks. In addition, we found that there was no change in the shape of the micro-cracks with additional heat treatment, if the residual tensile stress was sufficiently relieved by the micro-cracks formed in the thin film. The slits between the micro-cracks in the outdoor handle cover allowed the Capacitance Sensor and NFC to perform.
Electron Irradiation Effects on the Optical and Electrical Properties of ZnO/Ag/SnO2 Thin Films
Authors: Kim Yu-Sung , Choi Jin-Young , park Yun-je , Choe Su-Hyeon , Cha Byung-Chul , Kong Young-Min , Kim Daeil
Published: March 2020

Transparent conductive ZnO 50 nm/Ag 10 nm/SnO2 50 nm (ZAS) tri-layer films were deposited on glass substrates by magnetron sputtering, and then the surface was subjected to intense electron beam irradiation to investigate the effects of electron irradiation on the structural, optical, and electrical properties of the films. After deposition, the ZAS thin films were electron-irradiated for 10 minutes, with varying electron incident energies of 300, 600, and 900 eV. The films that were electron irradiated at 900 eV showed higher optical transmittance of 83.6% in the visible wavelength region, and lower resistivity, of 4.75 × 10-5 Ωcm, than the other films. From the observed electrical properties and optical band gap, it was concluded that the optical band gap increased with the incident electron energy up to 600 eV. The optical band gap increased from 4.12 to 4.23 eV, with carrier density increasing from 7.09 to 8.55 × 1021 cm−3. However, the film electron irradiated at 900 eV showed a decrease in optical band gap energy of 4.16 eV due to the decreased carrier density of 8.25 × 1021 cm−3. The figure of merit revealed that the ZAS thin films electron-irradiated at 900 eV had higher optical and electrical performance than the other films prepared in this study.
Vampires, Demons and the Disappearing Shadow in Folkloric Fictions of the Long Nineteenth Century
Authors: George Sam
Published: March 2020

This article interrogates manifestations of the shadow soul, combining anthropological, magical and religious approaches to the shadow in an analysis of a small group of interrelated Gothic texts. It enters the shadowy worlds of Peter Schlemihl, protagonist of Hans Andersen's ‘The Shadow’, Dracula, and Peter Pan, who, on being detached from their shadows, have themselves become shades. This journey into the kingdom of shadows will uncover the folkloric demon, the vampire, the physiognomist, the satyr, the pagan Pan, and the Devil himself. To read these narratives through Frazer's The Golden Bough challenges conventional psychoanalytic readings and offers an alternative, folkloric perspective, one which lays bare the ritualistic and magical beliefs that inform late nineteenth- and early twentieth-century Gothic.
Microbiota Characterization of Agricultural Green Waste-Based Suppressive Composts Using Omics and Classic Approaches
Authors: Scotti Riccardo , Mitchell Alex L. , Pane Catello , Finn Rob D. , Zaccardelli Massimo
Published: March 2020

While the control of soil-borne phytopathogenic fungi becomes increasingly difficult without using chemicals, concern over the intensive use of pesticides in agriculture is driving more environmentally sound crop protection managements. Among these approaches, the use of compost to suppress fungal diseases could have great potential. In this study, a multidisciplinary approach has been applied to characterize microbiota composition of two on-farm composts and assess their suppress and biostimulant activities. The on-farm composting system used in this study was able to produce two composts characterized by an antagonistic microbiota community able to suppress plant pathogens and biostimulate plant growth. Our results suggest a potential role for Nocardiopsis and Pseudomonas genera in suppression, while Flavobacterium and Streptomyces genera seem to be potentially involved in plant biostimulation. In conclusion, this study combines different techniques to characterize composts, giving a unique overview on the microbial communities and their role in suppressiveness, helping to unravel their complexity.