bluuebunny/crossref_metadata_2025 huggingface dataset:
Particulate Matter in Human Elderly: Higher Susceptibility to Cognitive Decline and Age-Related Diseases
Authors: López-Granero Caridad , Polyanskaya Leona , Ruiz-Sobremazas Diego , Barrasa Angel , Aschner Michael , Alique Matilde
Published: December 2023

This review highlights the significant impact of air quality, specifically particulate matter (PM), on cognitive decline and age-related diseases in the elderly. Despite established links to other pathologies, such as respiratory and cardiovascular illnesses, there is a pressing need for increased attention to the association between air pollution and cognitive aging, given the rising prevalence of neurocognitive disorders. PM sources are from diverse origins, including industrial activities and combustion engines, categorized into PM10, PM2.5, and ultrafine PM (UFPM), and emphasized health risks from both outdoor and indoor exposure. Long-term PM exposure, notably PM2.5, has correlated with declines in cognitive function, with a specific vulnerability observed in women. Recently, extracellular vesicles (EVs) have been explored due to the interplay between them, PM exposure, and human aging, highlighting the crucial role of EVs, especially exosomes, in mediating the complex relationship between PM exposure and chronic diseases, particularly neurological disorders. To sum up, we have compiled the pieces of evidence that show the potential contribution of PM exposure to cognitive aging and the role of EVs in mediating PM-induced cognitive impairment, which presents a promising avenue for future research and development of therapeutic strategies. Finally, this review emphasizes the need for policy changes and increased public awareness to mitigate air pollution, especially among vulnerable populations such as the elderly.
The Role of the Complement System in the Pathogenesis of Infectious Forms of Hemolytic Uremic Syndrome
Authors: Avdonin Piotr P. , Blinova Maria S. , Generalova Galina A. , Emirova Khadizha M. , Avdonin Pavel V.
Published: December 2023

Hemolytic uremic syndrome (HUS) is an acute disease and the most common cause of childhood acute renal failure. HUS is characterized by a triad of symptoms: microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury. In most of the cases, HUS occurs as a result of infection caused by Shiga toxin-producing microbes: hemorrhagic Escherichia coli and Shigella dysenteriae type 1. They account for up to 90% of all cases of HUS. The remaining 10% of cases grouped under the general term atypical HUS represent a heterogeneous group of diseases with similar clinical signs. Emerging evidence suggests that in addition to E. coli and S. dysenteriae type 1, a variety of bacterial and viral infections can cause the development of HUS. In particular, infectious diseases act as the main cause of aHUS recurrence. The pathogenesis of most cases of atypical HUS is based on congenital or acquired defects of complement system. This review presents summarized data from recent studies, suggesting that complement dysregulation is a key pathogenetic factor in various types of infection-induced HUS. Separate links in the complement system are considered, the damage of which during bacterial and viral infections can lead to complement hyperactivation following by microvascular endothelial injury and development of acute renal failure.
The Impact of Extraversion and Introversion on Millennials Propensity to Recommend Their Preferred Fitness Center
Authors: Dabija Dan-Cristian , Csorba Luiela Magdalena , Pop Nicolae Horațiu , Obadă Daniel-Rareș
Published: December 2023

Millennials of the 21st century tend to have an active daily life and practice more sports, spending more on health and fitness. Therefore, the present paper seeks to investigate the propensity of Millennials to recommend their preferred fitness center, depending on their psychological condition (i.e., introversion versus extraversion), which may lead to their tendency to practice and to their involvement and satisfaction with the fitness center. The authors deduce a conceptual model from the literature, which is further analyzed with data collected through an empirical investigation based on a face-to-face administered questionnaire. Data gathered from 2138 Millennials are investigated with the help of structural equation modeling using SmartPLS. The results show that a positive image of a fitness center stimulates the satisfaction of extravert and introvert consumers. Millennials have different personality types, such as extraversion and introversion, that influence their propensity to practice fitness and to recommend their favorite fitness center. It can be concluded that not only extraversion but also introversion influence consumers’ satisfaction, positively impacting the consumer’s intention to recommend their favorite fitness center to peers.
Simplified DEM-Based Modelling and Simulation of the Track Ballast
Authors: Kwon Youngwoong , Song Jihun , Lim Nam-Hyoung , Kim Seungjun
Published: December 2023

This study proposes a rational method for modelling ballast in tracks based on the discrete element method (DEM) approach. To simulate a ballast consisting of a group of gravels, it is essential to use the discrete element method. The ballast comprises randomly arranged gravels of unspecified shapes, and requires multiple steps and a separate house code for implementing the ballast. In this study, a simplified ballast analysis model using the parallel bond method (PBM) is proposed and verified through comparison with design standards. Additionally, case studies are conducted to investigate the effect of particle size distribution and micromechanical parameters on the simulation results.
Experimental Analysis of Mechanical Behavior of Timber-Concrete Composite Beams with Different Connecting Systems
Authors: Cvetković Radovan , Ranković Slobodan , Mišulić Tatjana Kočetov , Kukaras Danijel
Published: December 2023

Timber-concrete composite structures are innovative structural systems which have become the subject of extensive research and practical usage, primarily due to their attractive mechanical properties. This article deals with the experimental procedure and the analysis of the mechanical behavior of two different series of timber-concrete composite beams with the same span and geometry of cross-sections. In the first BF-series, the screws were used as a connecting system between the timber and concrete parts, whereas in the BN-series the combination of notches and screws, as a more complex system, was used for the same purpose. Both series were exposed to loading up to a failure by means of the standard four-point bending test. The mechanical behavior of the BF and BN-series beams was analyzed by a comparative analysis referring to: the correlation of the failure loading and the deflection, mechanisms of failure, the strain development across the height of mid-span’s and support’s cross-sections, the horizontal displacement in the timber-concrete interlayer at the support zones, the value of shear stresses and the calculated values of the effective bending stiffness of the beams. The differences in bearing capacity between both series of beams were negligible (about 5%), the effective bending stiffness of BF beams is lower for 32.86% compared to the BN-series and the average value of deflections in BF-series beams is twice as high than in the BN-series. The BN-series beams showed better mechanical behavior in aspects of development of shear stresses in support zones, exhibiting lower shear stress values with an average of 40%.
Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with PLK1 Variant rs27770A>G
Authors: Liao Zhouxiang , Zhang Qi , Yang Lichao , Li Hui , Mo Wanling , Song Zhenyu , Huang Xuejing , Wen Sha , Cheng Xiaojing , He Min
Published: December 2023

Hepatocellular carcinoma (HCC) has the highest incidence and mortality in the Asian population, and race is an independent risk factor affecting survival time in liver cancer. Micro RNAs (miRNAs) are remarkably dysregulated in HCC and closely associated with HCC prognosis. Recent studies show that genetic variability between ethnic groups may result in differences in the specificity of HCC miRNA biomarkers. Here, we reveal a high expression level of hsa-miR-100-5p, an HCC prognosis-related miRNA, which improves HCC prognosis in the Asian Population with Polo-like kinase 1 (PLK1) variant rs27770A>G. In this study, we discovered that hsa-miR-100-5p was downregulated in various HCC cell lines. While mimics transient transfection and mouse liver cancer model confirmed the interaction between hsa-miR-100-5p and PLK1, a stratified analysis based on the Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) data suggest both low hsa-miR-100-5p expression level and high PLK1 expression level associated with poor HCC prognosis, especially in the Asian population. According to the 1000 Genomes Project database, the SNP rs27770 located in 3′UTR of PLK1 had a significantly higher G allele frequency in the East Asian population. Bioinformatics analysis suggested that rs27770 A>G affects PLK1 mRNA secondary structure and alters the hsa-miR-100-5p/PLK1 interaction by forming an additional seedless binding site. This racial variation caused PLK1 to be more vulnerable to hsa-miR-100-5p inhibition, resulting in hsa-miR-100-5p being more favorable for HCC prognosis in the Asian population.
Genomic Landscape of Endometrial, Ovarian, and Cervical Cancers in Japan from the Database in the Center for Cancer Genomics and Advanced Therapeutics
Authors: Xi Qian , Kage Hidenori , Ogawa Miho , Matsunaga Asami , Nishijima Akira , Sone Kenbun , Kawana Kei , Oda Katsutoshi
Published: December 2023

This study aimed to comprehensively clarify the genomic landscape and its association with tumor mutational burden-high (TMB-H, ≥10 mut/Mb) and microsatellite instability-high (MSI-H) in endometrial, cervical, and ovarian cancers. We obtained genomic datasets of a comprehensive genomic profiling test, FoundationOne® CDx, with clinical information using the “Center for Cancer Genomics and Advanced Therapeutics” (C-CAT) database in Japan. Patients can undergo the tests only after standardized treatments under universal health insurance coverage. Endometrial cancers were characterized by a high frequency of TMB-H and MSI-H, especially in endometrioid carcinomas. The lower ratio of POLE exonuclease mutations and the higher ratio of TP53 mutations compared to previous reports suggested the prognostic effects of the molecular subtypes. Among the 839 cervical cancer samples, frequent mutations of KRAS, TP53, PIK3CA, STK11, CDKN2A, and ERBB2 were observed in adenocarcinomas, whereas the ratio of TMB-H was significantly higher in squamous cell carcinomas. Among the 1606 ovarian cancer samples, genomic profiling of serous, clear cell, endometrioid, and mucinous carcinomas was characterized. Pathogenic mutations in the POLE exonuclease domain were associated with high TMB, and the mutation ratio was low in both cervical and ovarian cancers. The C-CAT database is useful for determining the mutational landscape of each cancer type and histological subtype. As the dataset is exclusively collected from patients after the standardized treatments, the information on “druggable” alterations highlights the unmet needs for drug development in major gynecological cancers.
PI3Kδ Inhibition Potentiates Glucocorticoids in B-lymphoblastic Leukemia by Decreasing Receptor Phosphorylation and Enhancing Gene Regulation
Authors: Zimmerman Jessica A. O. , Fang Mimi , Pufall Miles A.
Published: December 2023

Glucocorticoids are the cornerstone of B-lymphoblastic leukemia (B-ALL) therapy. Because response to glucocorticoids alone predicts overall outcomes for B-ALL, enhancing glucocorticoid potency should improve treatment. We previously showed that inhibition of the lymphoid-restricted PI3Kδ with idelalisib enhances glucocorticoid activity in B-ALL cells. Here, we show that idelalisib enhances glucocorticoid potency in 90% of primary B-ALL specimens and is most pronounced at sub-saturating doses of glucocorticoids near the EC50. Potentiation is associated with enhanced regulation of all glucocorticoid-regulated genes, including genes that drive B-ALL cell death. Idelalisib reduces phosphorylation of the glucocorticoid receptor (GR) at PI3Kδ/MAPK1 (ERK2) targets S203 and S226. Ablation of these phospho-acceptor sites enhances sensitivity to glucocorticoids with ablation of S226 in particular reducing synergy. We also show that phosphorylation of S226 reduces the affinity of GR for DNA in vitro. We propose that PI3Kδ inhibition improves glucocorticoid efficacy in B-ALL in part by decreasing GR phosphorylation, increasing DNA binding affinity, and enhancing downstream gene regulation. This mechanism and the response of patient specimens suggest that idelalisib will benefit most patients with B-ALL, but particularly patients with less responsive, including high-risk, disease. This combination is also promising for the development of less toxic glucocorticoid-sparing therapies.
Dehydrogenation of Diethylene Glycol to Para-Dioxanone over Cu/SiO2 Catalyst: Effect of Structural and Surface Properties
Authors: Guo Kai , Wang Wanmin , Ye Yingchun , Chen Liangfeng , Wang Limin , Wang Jian , Zhu Junhua
Published: December 2023

Para-dioxanone, a typical monomer for high-performance biomedical polymers, is generally obtained from the catalytic dehydrogenation of diethylene glycol. In this work, Cu/SiO2 catalysts were prepared by two different methods and applied in diethylene glycol dehydrogenation. The effects of catalyst properties on the performance of para-dioxanone production were systematically explored by combined techniques. The results showed that the high specific surface area and regular pore structure of the catalyst promoted and stabilized the high dispersion of the copper species with an abundant Cu-Si interface, thereby providing numerous reactive sites. The appropriate Cu0/Cu+ ratio in the active components produced efficient synergy, which was responsible for the excellent dehydrogenation performance. In addition, the low surface acid density (0.532 μmol/m2) of the catalyst greatly reduced the occurrence of diethylene glycol dehydration, thus improving para-dioxanone selectivity over 90% with promising stability.
Amyloidogenic and Neuroinflammatory Molecular Pathways Are Contrasted Using Menaquinone 4 (MK4) and Reduced Menaquinone 7 (MK7R) in Association with Increased DNA Methylation in SK-N-BE Neuroblastoma Cell Line
Authors: Orticello Michela , Cavallaro Rosaria A. , Antinori Daniele , Raia Tiziana , Lucarelli Marco , Fuso Andrea
Published: December 2023

Besides its role in coagulation, vitamin K seems to be involved in various other mechanisms, including inflammation and age-related diseases, also at the level of gene expression. This work examined the roles of two vitamin K2 (menaquinones) vitamers, namely, menaquinone-4 (MK4) and reduced menaquinone-7 (MK7R), as gene modulator compounds, as well as their potential role in the epigenetic regulation of genes involved in amyloidogenesis and neuroinflammation. The SK-N-BE human neuroblastoma cells provided a “first-line” model for screening the neuroinflammatory and neurodegenerative molecular pathways. MK7R, being a new vitamin K form, was first tested in terms of solubilization, uptake and cell viability, together with MK4 as an endogenous control. We assessed the expression of key factors in amyloidogenesis and neuroinflammation, observing that the MK7R treatment was associated with the downregulation of neurodegeneration- (PSEN1 and BACE1) and neuroinflammation- (IL-1β and IL-6) associated genes, whereas genes retaining protective roles toward amiloidogenesis were upregulated (ADAM10 and ADAM17). By profiling the DNA methylation patterns of genes known to be epigenetically regulated, we observed a correlation between hypermethylation and the downregulation of PSEN1, IL-1β and IL-6. These results suggest a possible role of MK7R in the treatment of cognitive impairment, giving a possible base for further preclinical experiments in animal models of neurodegenerative disease.
The Effect of Temperature on the Surface Energetic Properties of Carbon Fibers Using Inverse Gas Chromatography
Authors: Hamieh Tayssir
Published: December 2023

This paper constitutes an original and new methodology for the determination of the surface properties of carbon fibers in two forms, namely, oxidized and untreated, using the inverse gas chromatography technique at infinite dilution based on the effect of temperature on the surface area of various organic molecules adsorbed on the carbon fibers. The studied thermal effect showed a large deviation from the classical methods or models relative to the new determination of the surface properties of carbon fibers, such as the dispersive component of their surface energy, the free surface energy, the free specific energy, and the enthalpy and entropy of the adsorption of molecules on the carbon fibers. It was highlighted that the variations in the London dispersive surface energy of the carbon fibers as a function of the temperature satisfied excellent linear variations by showing large deviations between the values of γsd (T), calculated using different models, which can reach 300% in the case of the spherical model. All models and chromatographic methods showed that the oxidized carbon fibers gave larger specific free enthalpy of adsorption whatever the adsorbed polar molecules. The obtained specific enthalpy and entropy of the adsorption of the polar solvents led to the determination of the Lewis acid–base constants of the carbon fibers. Different molecular models and chromatographic methods were used to quantify the surface thermodynamic properties of the carbon fibers, and the results were compared with those of the thermal model. The obtained results show that the oxidized carbon fibers gave more specific interaction energy and greater acid–base constants than the untreated carbon fibers, thus highlighting the important role of oxidization in the acid–base of fibers. The determination of the specific acid–base surface energy of the two carbon fibers showed greater values for the oxidized carbon fibers than for the untreated carbon fibers. An important basic character was highlighted for the two studied carbon fibers, which was larger than the acidic character. It was observed that the carbon fibers were 1.4 times more acidic and 2.4 times more basic. The amphoteric character of the oxidized fibers was determined, and it was 1.7 times more important than that of the untreated fibers This tendency was confirmed by all molecular models and chromatographic methods. The Lewis acid and base surface energies of the solid surface, γs+ and γs−, as well as the specific acid–base surface energy γsAB of the carbon fibers at different temperatures were determined. One showed that the specific surface energy γsAB of the oxidized fibers was 1.5 times larger than that of the untreated fibers, confirming the above results obtained on the strong acid–base interactions of the oxidized carbon fibers with the various polar molecules.
The Transition from Type-I to Type-II SiC/GaN Heterostructure with External Strain
Authors: Zhang Li , Sun Haiyang , Zheng Ruxin , Pan Hao , Mu Weihua , Wang Li
Published: December 2023

Two-dimensional materials are widely used as a new generation of functional materials for photovoltaic, photocatalyst, and nano-power devices. Strain engineering is a popular method to tune the properties of two-dimensional materials so that performances can be improved or more applications can be obtained. In this work, a two-dimensional heterostructure is constructed from SiC and GaN monolayers. Using first-principle calculations, the SiC/GaN heterostructure is stacked by a van der Waals interaction, acting as a semiconductor with an indirect bandgap of 3.331 eV. Importantly, the SiC/GaN heterostructure possesses a type-II band structure. Thus, the photogenerated electron and hole can be separated in the heterostructure as a potential photocatalyst for water splitting. Then, the external biaxial strain can decrease the bandgap of the SiC/GaN heterostructure. From pressure to tension, the SiC/GaN heterostructure realizes a transformation from a type-II to a type-I semiconductor. The strained SiC/GaN heterostructure also shows suitable band alignment to promote the redox of water splitting at pH 0 and 7. Moreover, the enhanced light-absorption properties further explain the SiC/GaN heterostructure’s potential as a photocatalyst and for nanoelectronics.
Experimental Investigation of Laser Damage Limit for ZPG Infrared Single Crystal Using Deep Magnetorheological Polishing of Working Surfaces
Authors: Yudin Nikolay N. , Khudoley Andrei , Zinovev Mikhail , Slyunko Elena , Podzyvalov Sergey , Kuznetsov Vladimir , Gorodkin Gennady , Kumeysha Pavel , Lysenko Alexey , Kalsin Andrey , Gabdrakhmanov Akmal , Romanovskii Oleg A. , Kashevsky Sergey , Baalbaki Houssain
Published: December 2023

Zinc germanium phosphide (ZGP) crystals have garnered significant attention for their nonlinear properties, making them good candidates for powerful mid-IR optical parametric oscillators and second-harmonic generators. A ZnGeP2 single crystal was treated by deep magnetorheological processing (MRP) until an Angstrom level of roughness. The studies presented in this article are devoted to the experimental evaluation of the influence of deep removal (up to 150 μm) from the surface of a ZnGeP2 single crystal by magnetorheological polishing on the parameters of optical breakdown. It was shown that the dependence of the ZnGeP2 laser-induced damage threshold on MRP depth is a smooth monotonically decreasing logarithmic function. The obtained logarithmic dependence indicates the thermal nature of optical breakdown and the dependence of the ZnGeP2 laser-induced damage threshold on the concentration of surface absorbing defects.
U–Pb Dating, Gemology, and Chemical Composition of Apatite from Dara-e-Pech, Afghanistan
Authors: Lai Biying , Xu Bo , Zhao Yi
Published: December 2023

Minerals of the apatite group commonly occur in granite pegmatites, and their ability to incorporate a wide range of trace elements makes them a good indicator of magma composition and magmatic–hydrothermal processes. Gem-quality purple apatite crystals from the Dara-e-Pech pegmatite field in Afghanistan have rarely been reported. Here, we investigated apatite crystals originated from this locality, using gemological testing, chemical analysis, and in situ U–Pb dating, with the purpose of identifying their origin, the constraints on the magma source in which the apatite crystals were formed, and the timing of the magmatic–hydrothermal activity. Our findings demonstrate that the purple apatite crystals were impure fluorapatite, characterized by heavy rare-earth element (HREE) enrichment, intermediate Eu anomalies, and non-CHARAC Y/Ho ratios. The results showed that these apatite crystals yielded a lower intercept age of 135.8 ± 6.9 Ma. We proposed that the pegmatitic apatite samples formed in a transitional magmatic–hydrothermal pegmatitic system with moderate fO2 in the Early Cretaceous (~135 Ma). Our study helps to constrain the magmatic–hydrothermal activities of the little-known Dara-e-Pech pegmatite field.
Prognostic Impact of a Routine Six-Month Exercise Stress Test after Complex Left Main Bifurcation Percutaneous Intervention
Authors: Rigatelli Gianluca , Zuin Marco , Marchese Giuseppe , Hiso Ervis , Rodinò Giulio , Roncon Loris , Pasquetto Giampaolo
Published: December 2023

The prognostic value of exercise stress test after complex left main (LM) coronary artery bifurcation (LM) stenting has been poorly investigated. To partially fill this gap in knowledge, we retrospectively analyzed the procedural and medical data of consecutive patients referred to our center for complex LM bifurcation disease between January 2008 and May 2018 who were treated using either single- or dual-stenting techniques. The prognostic impact of an exercise stress test, performed 6 months after the coronary intervention, was evaluated in 502 patients (316 males, mean age 70.3 ± 12.8 years, mean Syntax score 31.6 ± 6.3). At follow up after a mean of 37.1 ± 10.8 months (range 22.1–47.3 months), the target lesion failure (TLF) rate was 10.1% while stent thrombosis and cardiovascular mortality were 1.2 and 3.6%, respectively. A positive exercise stress test was detected at 6-month follow up in 42 out of 502 patients (8.4%); the incidence of a significant restenosis was 7.6% (n = 38). Patients with a negative exercise stress test at 6-month follow up had higher freedom from TLF and improved survival compared to those with a positive exercise stress test.
Comparing Diagnostic Performance of Short and Long [18F]FDG-PET Acquisition Times in Giant Cell Arteritis
Authors: Nienhuis Pieter H. , van Nieuwland Marieke , van Praagh Gijs D. , Markusiewicz Karolina , Colin Edgar M. , van der Geest Kornelis S. M. , Wagenaar Nils , Brouwer Elisabeth , Alves Celina , Slart Riemer H. J. A.
Published: December 2023

(1) Background: In giant cell arteritis (GCA), the assessment of cranial arteries using [18F]fluorodeoxyglucose ([18F]FDG) positron emission tomography (PET) combined with low-dose computed tomography (CT) may be challenging due to low image quality. This study aimed to investigate the effect of prolonged acquisition time on the diagnostic performance of [18F]FDG PET/CT in GCA. (2) Methods: Patients with suspected GCA underwent [18F]FDG-PET imaging with a short acquisition time (SAT) and long acquisition time (LAT). Two nuclear medicine physicians (NMPs) reported the presence or absence of GCA according to the overall image impression (gestalt) and total vascular score (TVS) of the cranial arteries. Inter-observer agreement and intra-observer agreement were assessed. (3) Results: In total, 38 patients were included, of whom 20 were diagnosed with GCA and 18 were without it. Sensitivity and specificity for GCA on SAT scans were 80% and 72%, respectively, for the first NMP, and 55% and 89% for the second NMP. On the LAT scans, these values were 65% and 83%, and 75% and 83%, respectively. When using the TVS, LAT scans showed especially increased specificity (94% for both NMPs). Observer agreement was higher on the LAT scans compared with that on the SAT scan. (4) Conclusions: LAT combined with the use of the TVS may decrease the number of false-positive assessments of [18F]FDG PET/CT. Additionally, LAT and TVS may increase both inter and intra-observer agreement.
Improving Electric Vehicle Structural-Borne Noise Based on Convolutional Neural Network-Support Vector Regression
Authors: Jia Xiaoli , Zhou Lin , Huang Haibo , Pang Jian , Yang Liang
Published: December 2023

In order to enhance the predictive accuracy and control capabilities pertaining to low- and medium-frequency road noise in automotive contexts, this study introduces a methodology for Structural-borne Road Noise (SRN) prediction and optimization. This approach relies on a multi-level target decomposition and a hybrid model combining Convolutional Neural Network (CNN) and Support Vector Regression (SVR). Initially, a multi-level target analysis method is proposed, grounded in the hierarchical decomposition of vehicle road noise along the chassis parts, delineated layer by layer, in accordance with the vibration transmission path. Subsequently, the CNN–SVR hybrid model, predicated on the multi-level target framework, is proposed. Notably, the hybrid model exhibits a superior predictive accuracy exceeding 0.97, surpassing both traditional CNN and SVR models. Finally, the method and model are deployed for sensitivity analysis of chassis parameters in relation to road noise, as well as for the prediction and optimization analysis of SRN in vehicles. The outcomes underscore the high sensitivity of parameters such as the dynamic stiffness of the rear axle bushing and the large front swing arm bushing influencing SRN. The optimization results, facilitated by the CNN–SVR hybrid model, align closely with the measured outcomes, displaying a negligible relative error of 0.82%. Furthermore, the measured results indicate a noteworthy enhancement of 4.07% in the driver’s right-ear Sound Pressure Level (SPL) following the proposed improvements compared to the original state.
Power-from-Shore Optioneering for Integration of Offshore Renewable Energy in Oil and Gas Production
Authors: Antunes Tiago A. , Castro Rui , Santos Paulo J. , Pires Armando J.
Published: December 2023

Despite the widespread usage of high-voltage alternating current (HVAC) for the connection of offshore wind farms (OWF), its use to power-from-shore (PFS) offshore oil and gas (O&G) production sites is often not feasible. Its limitations for long-distance subsea transmission are usually found at 50–70 km from shore and might be even shorter when compared commercially to a direct-current (DC) alternative or conventional generation. Therefore, this research paper aims to address the standardization of offshore transmission with a particular focus on the high-voltage direct current (HVDC) alternative. While the distance is typically not a limiting factor when using DC, and the voltages used are rather standard, the concept of power envelopes can be quite useful in addressing the high variability of offshore site power requirements and setting a design baseline that would lead to improved lead time. In this article, a full back and front-end genetic optioneering model purposely built from the ground up in Python language is used to #1 define up to three DC power envelopes that would cater to most of the candidate’s requirements and #2 provide the lowest cost variance. The results will demonstrate that this can be achieved at a minor overall cost expense.
A Fourier–Legendre Polynomial Forest Height Inversion Model Based on a Single-Baseline Configuration
Authors: Zhang Bing , Zhu Hongbo , Xu Wenxuan , Xu Sairu , Chang Xinyue , Song Weidong , Zhu Jianjun
Published: December 2023

In this article, we propose a Fourier–Legendre (FL) polynomial forest height estimation algorithm based on low-frequency single-baseline polarimetric interferometric synthetic aperture radar (PolInSAR) data. The algorithm can obtain forest height with a single-baseline PolInSAR configuration while capturing a high-resolution vertical profile for the forest volume. This is based on the consideration that the forest height remains constant within neighboring pixels. Meanwhile, we also assume that the coefficients of the FL polynomials remain unchanged within neighboring pixels, except for the last polynomial coefficient. The idea of using neighboring pixels to increase the observations provides us with the possibility to obtain high-order FL polynomials. With this approach, it is possible to obtain a high-resolution vertical profile that is suitable for forest height estimation without losing too much spatial resolution. P-band PolInSAR data acquired in Mabounie in Gabon and Krycklan in Sweden were selected for testing the proposed algorithm. The results show that the algorithm outperforms the random volume over ground (RVoG) model by 18% and 16.7% in forest height estimation for the Mabounie and Krycklan study sites, respectively.
Botryosphaeriaceae Species Associated with Stem Canker, Shoot Blight and Dieback of Fraxinus ornus in Italy
Authors: Benigno Alessandra , Aglietti Chiara , Rossetto Giovanni , Bregant Carlo , Linaldeddu Benedetto Teodoro , Moricca Salvatore
Published: December 2023

A severe dieback of flowering ash (Fraxinus ornus L.) has been observed in north-central Italy in the last decades. Symptoms include typical sunken, light-brown cankers on the stem and branches; vascular discoloration; tip and shoot dieback; and foliage necroses. The disease was more evident at the beginning of the growing season, and more severe on young regeneration. Six Botryosphaeriaceae species were consistently isolated from symptomatic plant tissues: Botryosphaeria dothidea, Diplodia fraxini, Diplodia subglobosa, Dothiorella iberica, Dothiorella omnivora and Neofusicoccum parvum. B. dothidea and D. fraxini expressed higher aggressiveness and showed a widespread incidence, being the species most frequently associated with cankers; the other four species were less virulent and more erratic, occurring mainly on succulent branch tips and foliage. Isolates were characterized using morphological and molecular approaches (colony/conidial phenotyping and rDNA-ITS genotyping). Phylogenetic analysis provided congruent phylogenies depicting the relationships of the six taxa with the most closely related conspecifics. Pathogenicity tests on 2-year-old seedlings confirmed the higher virulence of B. dothidea and D. fraxini. Extensive, multi-year field surveys at different sites supported the hypothesis that climatic vagaries, mainly heat, water and drought stresses, impaired tree health and vigor, facilitating infection and pervasive colonization by these Botryosphaeriaceae species. Environmental stressors are thus the key factor bringing the six fungal pathogens together in a multitrophic interaction with F. ornus in a novel, lethal fashion.
Theoretical and Experimental Investigations of Oxygen Activation Effect of Carbon Nanofibers Interacting with Polypyrrole
Authors: Xie Yibing , Wang Yiting , Wang Lijun , Liang Jiawei
Published: December 2023

Theoretical modeling calculations and experimental measurements were adopted to investigate the oxygen activation effect of carbon nanofibers (CNFs) interacting with polypyrrole (PPY). The CNF undergoes a hydrothermal oxidation process to form epoxy and hydroxyl groups containing carbon nanofibers (CNF-O). The oxygen activation effect of CNF on the electronic and electrochemical properties was investigated through the interfacial interaction between CNF-O and PPY. Theoretical modeling calculation discloses that CNF-O/PPY exhibits lower electronic bandgaps (0.64 eV), a higher density of states (10.039 states/eV), and a lower HOMO–LUMO molecular orbital energy gap (0.077 eV) than CNF/PPY (1.56 eV, 7.946 states/eV and 0.112 eV), presenting its superior electronic conductivity and electroactivity. The Mulliken population and charge density difference analysis disclose the stronger interface interaction of CNF-O/PPY caused by epoxy and hydroxyl groups. Cyclic voltammogram measurements reveal that CNF-O/PPY exhibits a higher response current and a higher specific capacitance (221.1–112.2 mF g−1) than CNF/PPY (57.6–24.2 mF g−1) at scan rates of 5–200 mV s−1. Electrochemical impendence spectrum measurements disclose that CNF-O/PPY exhibits a lower charge transfer resistance (0.097 Ω), a lower ohmic resistance (0.336 Ω), a lower Warburg impedance (317 Ω), and a higher double-layer capacitance (0.113 mF) than CNF/PPY (1.419 Ω, 9.668 Ω, 7865 Ω, and 0.015 mF). Both theoretical and experimental investigations prove that CNF-O/PPY presents an intensified intermolecular interaction rather than CNF/PPY. The promotive oxygen activation effect of CNF could contribute to improving the electronic and electrochemical properties of CNF-O/PPY.
Effects of Calcium and pH on Rheological Thermal Resistance of Composite Xanthan Gum and High-Methoxyl Apple Pectin Matrices Featuring Dysphagia-Friendly Consistency
Authors: Yang Huaiwen , Chou Liang-Yu , Hua Chi-Chung
Published: December 2023

High-methoxyl apple pectin (AP) derived from apple was employed as the main ingredient facilitating rheological modification features in developing dysphagia-friendly fluidized alimentary matrices. Xanthan gum (XG) was also included as a composite counterpart to modify the viscoelastic properties of the thickened system under different thermal processes. The results indicate that AP is extremely sensitive to thermal processing, and the viscosity is greatly depleted under a neutral pH level. Moreover, the inclusion of calcium ions echoed the modification effect on the rheological properties of AP, and both the elastic property and viscosity value were promoted after thermal processing. The modification effect of viscoelastic properties (G′ and G″) was observed whne XG was incorporated into the composite formula. Increasing the XG ratio from 7:3 to 6:4 (AP:XG) triggers the rheological transformation from a liquid-like form to a solid-like state, and the viscosity value shows that the AP-XG composite system exhibits better thermal stability after thermal processing. The ambient modifiers of pH (pH < 4) and calcium chloride concentration (7.5%) with an optimal AP-XG ratio of 7:3 led to weak-gel-like behavior (G″ < G′), helping to maintain the texture properties of dysphagia-friendly features similar to those prior to the thermal processing.
Characterization of Băbească Neagră Grape Pomace and Incorporation into Jelly Candy: Evaluation of Phytochemical, Sensory, and Textural Properties
Authors: Spinei Mariana , Oroian Mircea
Published: December 2023

The influence of particle size intervals (<125 μm, ≥125–<200 μm, and ≥200–<300 μm) of grape pomace (Vitis vinifera var. Băbească Neagră) was analyzed in terms of the proximate composition, functional properties, and physicochemical parameters. The aim was to study the effect of the formulation variables (extract from grape pomace with different particle size intervals and gelatin doses—7, 8.5, and 10 g) on the mechanical properties (hardness, cohesiveness, adhesiveness, springiness, and gumminess), color, and sensorial, microbiological, and phytochemical parameters. The jelly candy formulated with grape pomace extract (<125 μm) and 7 g of gelatin showed the highest total phenolic content (156 mg GAE/g) and antioxidant activity (65.8% inhibition), while grape pomace jellies with a particle size of ≥125–<200 μm and different concentration of gelatin presented the greatest sensory acceptance in terms of sweetness, taste, odor, elasticity, color, and overall acceptability of the resulting jelly. The concluding observation was supported by the microbiological analysis, which also showed that there is no growth in jelly samples except the jelly candies prepared with a ≥200–<300 μm particle size interval of grape pomace extract.
Bootstrapping State-Space Models: Distribution-Free Estimation in View of Prediction and Forecasting
Authors: Lima José Francisco , Pereira Fernanda Catarina , Gonçalves Arminda Manuela , Costa Marco
Published: December 2023

Linear models, seasonal autoregressive integrated moving average (SARIMA) models, and state-space models have been widely adopted to model and forecast economic data. While modeling using linear models and SARIMA models is well established in the literature, modeling using state-space models has been extended with the proposal of alternative estimation methods to the maximum likelihood. However, maximum likelihood estimation assumes, as a rule, that the errors are normal. This paper suggests implementing the bootstrap methodology, utilizing the model’s innovation representation, to derive distribution-free estimates—both point and interval—of the parameters in the time-varying state-space model. Additionally, it aims to estimate the standard errors of these parameters through the bootstrap methodology. The simulation study demonstrated that the distribution-free estimation, coupled with the bootstrap methodology, yields point forecasts with a lower mean-squared error, particularly for small time series or when dealing with smaller values of the autoregressive parameter in the state equation of state-space models. In this context, distribution-free estimation with the bootstrap methodology serves as an alternative to maximum likelihood estimation, eliminating the need for distributional assumptions. The application of this methodology to real data showed that it performed well when compared to the usual maximum likelihood estimation and even produced prediction intervals with a similar amplitude for the same level of confidence without any distributional assumptions about the errors.
The Western as a Genre of Cultural Mobility
Authors: Holtz Martin
Published: December 2023

The Western is, in many respects, the essential American film genre, “a cornerstone of American identity” (Kitses). Yet, despite its distinctly American character, the genre has exerted a fascination all over the world. This contribution examines the Western as a site of transnational cultural exchange and as an illustration of what Stephen Greenblatt calls cultural mobility. In the work of Akira Kurosawa’s Seven Samurai (1954) and Yojimbo (1961), Western elements are evoked, which provide complex comments on the influence of American culture on Japan in the post-WWII years. While Seven Samurai appears to embrace the promise of class eradication as a result of Westernization, its American remake The Magnificent Seven (1960) shows a particular fascination for the decidedly Japanese aspects of the material, namely the idea of a warrior class dissociated from society. The Italian remake of Yojimbo, Fistful of Dollars (1964), shows how the Western can function not only as an external comment on American culture, in its cynical redefinition of the cowboy hero, but also as an amalgam of cultural practices and symbols, reaching from Japanese samurai codes to Christian Catholic redeemer imagery, that through their stylization expose the performativity of culture as such.
First Record of Microplastic Contamination in the Non-Native Dark False Mussel Mytilopsis leucophaeata (Bivalvia: Dreissenidae) in a Coastal Urban Lagoon
Authors: Neves Raquel A. F. , Guimarães Tâmara B. , Santos Luciano N.
Published: December 2023

Microplastic contamination is a global concern due to its conspicuous presence in aquatic ecosystems and its toxic nature to environmental and human health. False mussels are among the most notable fresh- and brackish water invaders. The invasive Mytilopsis leucophaeata in Rodrigo de Freitas Lagoon-RFL (Rio de Janeiro, Brazil) is the most abundant macrofaunal invertebrate, widely established and distributed throughout the lagoon. This study aimed to assess microplastic contamination in this invasive filter feeder and evaluate its potential use as a bioindicator. Agglomerates (~100 mussels) were manually collected using a stainless-steel spatula in ten sampling areas distributed throughout the whole lagoon and kept frozen. In the laboratory, 60 individuals were sorted by area for soft-tissue digestion. Each pool of 10 soft-tissue mussels (n = 6 by area) was wet-weighted and then placed in a 150-mL decontaminated glass beaker with 50 mL of 10% KOH. Samples were heated (40 °C) for 48 h, and digested samples were filtered in glass-fiber membranes. Microplastics were found in all samples of mussels (n = 60) from RFL; the particles were mostly lower than 100 µm with a mean concentration (±SD) of 35.96 ± 47.64 MPs g wet-weight−1. Microplastics were distinguished in seven shapes with different occurrences in samples (%): fiber (43.3%); fragment (34.3%); film (16.3%); sponge/foam (4.9%); pellet (0.57%), rope/filaments (0.17%); and undefined (0.4%). Thirteen colors of microplastics were found, but transparent (54.94%), black (10.77%), and white (9.36%) were the most common. Mytilopsis leucophaeata were useful to assess microplastic contamination in RFL and might be preferentially used in other invaded brackish systems instead of native and often threatened bivalves. Our results confirm the effective application of bivalves as an indicator of coastal microplastic pollution.
Applications of Genome-Editing Technologies for Type 1 Diabetes
Authors: El Nahas Rana , Al-Aghbar Mohammad Ameen , Herrero Laura , van Panhuys Nicholas , Espino-Guarch Meritxell
Published: December 2023

Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by the destruction of insulin-producing pancreatic β-cells by the immune system. Although conventional therapeutic modalities, such as insulin injection, remain a mainstay, recent years have witnessed the emergence of novel treatment approaches encompassing immunomodulatory therapies, such as stem cell and β-cell transplantation, along with revolutionary gene-editing techniques. Notably, recent research endeavors have enabled the reshaping of the T-cell repertoire, leading to the prevention of T1D development. Furthermore, CRISPR–Cas9 technology has demonstrated remarkable potential in targeting endogenous gene activation, ushering in a promising avenue for the precise guidance of mesenchymal stem cells (MSCs) toward differentiation into insulin-producing cells. This innovative approach holds substantial promise for the treatment of T1D. In this review, we focus on studies that have developed T1D models and treatments using gene-editing systems.
Incidental Bystander or Essential Culprit: A Systematic Review of Bacterial Significance in the Pathogenesis of Breast Implant-Associated Anaplastic Large Cell Lymphoma
Authors: Foppiani Jose A. , Raska Otakar , Taritsa Iulianna , Hernandez Alvarez Angelica , Lee Daniela , Escobar-Domingo Maria J. , Berger Josephine , Klener Pavel , Schuster Kirsten A. , Abdo Daoud , Clemens Mark W. , Lin Samuel J.
Published: December 2023

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a distinct subtype of T-cell non-Hodgkin lymphoma that arises in the context of prolonged exposure to textured breast implants. The intent of this manuscript is to explore whether the bacterial presence in biofilms on these implants is a mere incidental finding or plays a pivotal role in the pathogenesis of BIA-ALCL. Our goal is to delineate the extent of bacterial involvement, offering insights into potential underlying mechanisms, and establishing future research priorities aimed at resolving the remaining uncertainties surrounding this complex association. A comprehensive systematic review of several databases was performed. The search strategy was designed and conducted by an experienced librarian using controlled vocabulary with keywords. The electronic search identified 442 publications. After evaluation, six studies from 2015 to 2021 were included, encompassing 201 female patients aged 23 to 75. The diagnosis span post-implantation ranged from 53 to 135.6 months. Studies consistently found bacteria near breast implants in both BIA-ALCL cases and controls, with varied microbial findings. Both BIA-ALCL cases and controls exhibited the presence of specific bacteria, including Pseudomonas aeruginosa, Klebsiella oxytoca, Staphylococcus aureus, and Ralstonia spp., without any statistically significant differences between groups. The use of antiseptic and antimicrobial agents during implant insertion did not demonstrate any impact on reducing or altering the risk of developing BIA-ALCL. Our systematic review reveals that the current evidence is inadequate to link bacterial etiology as a central factor in the development of BIA-ALCL. The limitations in the existing data prevent a complete dismissal of the role of biofilms in its pathogenesis. The observed gap in knowledge underscores the need for more focused and comprehensive research, which should be structured in a multi-faceted approach. Initially, this involves the utilization of sophisticated genomic and proteomic methods. Following this, it is crucial to delve into the study of immunological reactions specifically induced by biofilms. Finally, this research should incorporate extended observational studies, meticulously tracking the evolution of biofilm development and its correlation with the emergence of BIA-ALCL. In light of the inconclusive nature of current findings, further investigation is not only justified but urgently needed to clarify these unresolved issues.