bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis berfikir matematis pada hasil evaluasi belajar siswa SMP Negeri 2 Sanden pada pembelajaran daring di masa pandemi covid-19
Authors: Astriantono Prabowo Wahid Riski , Palupi Sri Wijayanti
Published: January 2025

Penelitian ini bertujuan untuk memetakan kemampuan berfikir matematis siswa selama pembelajaran daring di masa pendemi Covid-19 sehingga dapat menjadi bahan evaluasi proses pembelajaran di SMP Negeri 2 Sanden. Jenis penelitian ini merupakan penelitian deskriptif kuantitatif. Subjek dalam penelitian ini adalah seluruh siswa kelas VII F SMP Negeri 2 Sanden. Instrumen yang digunakan dalam penelitian ini adalah data tugas subjek penelitian adalah tugas matematika siswa, angket kemampuan berfikir matematis siswa pada pembelajaran pembelajaran daring di semester genap pada tahun 2022 selama Covid-19, wawancara yang dilakukan secara langsung disekolah dan dokumentasi. Kemampuan berfikir matematis ditentukan oleh banyaknya indikator kemampuan berfikir matematis yang terpenuhi yaitu kemampuan pemahaman matematis, kemampuan pemecahan masalah, kemampuan penalaran matematis, kemampuan koneksi matematis, dan kemampuan komunikasi matematis.  Hasil penelitian menunjukkan bahwa siswa yang memiliki kemampuan berfikir matematis siswa dalam kategori tinggi adalah siswa yang dapat menyelesaikan soal dengan memenuhi 53,9% indikator. Kemampuan berfikir matematis siswa dalam kategori sedang adalah siswa yang dapat menyelesaikan soal dengan memenuhi 45,2% indikator dari kelima kemampuan. Kemampuan berfikir matematis siswa dalam kategori rendah adalah siswa yang dapat menyelesaikan soal dengan memenuhi 32,8% indikator dari kelima kemampuan.
Avanços no tratamento da Leucemia Mieloide Aguda: uma revisão de literatura
Authors: Rocha Fernando Lacerda da , Maciel Talitta Alves , Morgensztern Rodrigo Zatyrko , Fernandes Danilo Diogo , Guimarães Vinícius De Simoni , Sampaio Lívia Diniz , Alves Gabriel Rodrigues , Oliveira Ruan Victor e Silva , Santos Catarina Castro dos , Abreu Fernanda Garzedim Santos de , Costa Rafael Ferian , Ferian Héllen Jacquelline Orestes , Rubiño Roberto Silva , Tatto Lenin Batista , Trigo José Henrique Bispo , Piai Ana Vitória Brandão , Coelho Paulo Renato Mattos , Oliveira Ana Lucia Rebelo , Santos Ryan Pereira dos , Iório Kevin Ian Meira , Brollo Liliana Malaman
Published: January 2025

A leucemia mieloide aguda (LMA) é uma neoplasia agressiva com alta mortalidade, particularmente em pacientes de alto risco. Avanços recentes no tratamento têm transformado a abordagem da doença, com destaque para terapias direcionadas, como inibidores de FLT3, IDH1/2 e menin, além de combinações de venetoclax com agentes hipometilantes, que demonstraram altas taxas de remissão, especialmente em pacientes frágeis. Imunoterapias, como o magrolimab e CAR-T, também emergiram como alternativas promissoras. Além disso, estratégias como manutenção pós-transplante e tecnologias avançadas, incluindo sequenciamento genômico e inteligência artificial, têm contribuído para a prevenção de recaídas e o manejo da doença residual mínima. Esses avanços estão promovendo maior personalização no tratamento e melhorias nos desfechos clínicos, embora desafios significativos ainda precisem ser enfrentados.
Navigating the Future of Human Capital: Driving Operational Success in 2025 and Beyond Through a Talent-Centric Approach
Authors: Westover Jonathan
Published: January 2025

The future of work promises dramatic changes to human capital management, with technological advances and generational shifts disrupting traditional jobs and skills requirements. To succeed amid this disruption, organizations must adopt talent-centric strategies that focus on developing employees as a key asset. This involves rethinking the employee-employer relationship, shifting to a "talent mindset" that emphasizes continuous development, flexible career paths, and a holistic employee experience. Innovative recruitment strategies leveraging alternative data and immersive experiences will be needed to source in-demand skills. Continuous reskilling and upskilling initiatives, including micro-credentials and mentorship programs, will be critical. Nurturing inclusive, multi-generational teams that leverage diverse perspectives will also be essential. Enabling internal talent mobility and embedding diversity, equity and inclusion further strengthen the talent pipeline. Finally, the ethical deployment of emerging technologies, with a focus on augmenting rather than replacing human skills, is crucial. Organizations that proactively implement these talent-centric strategies will be well-positioned to navigate the evolving workforce landscape of 2025 and beyond.
Cultivating Inclusive, Innovative, and Resilient Organizations through Strategic Human Capital Initiatives
Authors: Westover Jonathan
Published: January 2025

In today's dynamic business environment, organizations face the dual challenge of navigating complex changes while also cultivating a thriving, engaged, and future-ready workforce. This research brief provides a comprehensive roadmap for leaders and human capital professionals seeking to build inclusive, innovative, and resilient organizations. The brief covers key strategies such as forging impactful diversity, equity, and inclusion (DEI) partnerships, navigating transformative organizational change, developing future-ready leadership, unlocking the "blue ocean" advantage, and fostering a learning culture. It also highlights the importance of enhancing employee engagement and well-being through ethical leadership, work-life balance, and positive work connections. By leveraging these strategic human capital initiatives, organizations can unlock the full potential of their workforce and position themselves for sustainable growth and long-term success.
ANALISIS EKSPRESIF LIRIK LAGU “TUTUR BATIN” KARYA YURA YUNITA DENGAN PENDEKATAN PRAKMATIK
Authors: Putri Risfani
Published: January 2025

Penelitian ini bertujuan untuk menganalisis lirik lagu "Tutur Batin" karya Yura Yunita menggunakan pendekatan pragmatik dengan fokus pada analisis tindak tutur dan ekspresi emosional yang terkandung dalam liriknya. Jenis penelitian yang digunakan adalah deskriptif kualitatif, yang menggali makna-makna tersirat melalui analisis teks lagu. Data utama dalam penelitian ini adalah lirik lagu yang diambil dari platform musik dan situs lirik lagu. Penelitian ini menggunakan teori pragmatik Austin (1962) dan analisis ekspresif untuk mengidentifikasi elemen-elemen pragmatik, seperti tindak tutur, implikatur, dan ekspresi emosional dalam lirik lagu. Hasil penelitian menunjukkan bahwa lirik lagu "Tutur Batin" mengandung ekspresi emosional seperti kekecewaan, keyakinan diri, trauma masa lalu, penerimaan diri, kebebasan emosional, dan perayaan hidup. Tindak tutur dalam lagu ini terbagi menjadi locutionary, illocutionary, dan perlocutionary acts yang mencerminkan komunikasi penyanyi dengan pendengarnya melalui pengungkapan perasaan dan keyakinan. Implikatur yang tersirat dalam lirik juga mencerminkan stigma sosial, penerimaan diri, dan identitas diri. Penelitian ini menekankan pentingnya makna yang disampaikan melalui bahasa dalam lirik lagu, yang berfungsi sebagai media ekspresi diri dan pemberdayaan emosional bagi pendengarnya.
Kata Kunci: Lirik Lagu Tutur Batin,  Analisis Ekspresif, Media Komunikasi Emosional
Sport Participation Positively Associated with Balance Confidence and Well-being in Transtibial Amputees
Authors: Hudson Lucas , Sanzo Paolo , Zerpa Carlos , Klarner Taryn
Published: January 2025

Transtibial amputation (TTA) is the removal of the lower leg often resulting in pain, mental health issues, and a more sedentary lifestyle that lacks physical activity (PA). Low balance confidence and other factors related to the physical and psychosocial adaptation to amputation could contribute to why people with TTA actively avoid PA. Studies have investigated lower extremity amputations and barriers to PA in general, but none have focused solely on transtibial amputation and its relationship with PA participation and avoidance habits. To address this issue, 21 participants with TTA (14 male, 6 female, and 1 non-binary) shared their experiences through an online survey. Relationships between variables were examined with Spearman and Pearson correlations. Results revealed a positive relationship, with a moderate effect size between balance confidence and the sport index scale and multiple other correlations between the physical and psychosocial adaptation to TTA and PA participation and avoidance. Those who participate in sport following TTA seem to show a positive association with social adjustment and balance confidence. In addition, adaptations such as adjusting to new limitations and activity restrictions, as well as satisfaction with the function of the prosthesis, are also related to PA participation and avoidance. This study supports the premise that participating in PA, specifically sport, has physical and psychosocial benefits that cannot be undervalued. Insights drawn from this study lend support to evidence-based practices of rehabilitation programs for people with TTA that focus on balance confidence training. If those with TTA are interested in sports, balance confidence training via sport-specific training could further facilitate a positive adaptation following TTA.
Origin and Development: The Evolution of the US–Japan–ROK Trilateral Security Mechanism
Authors: Zhao Lanyu
Published: January 2025

With the rise of global hot issues and the continuation of local wars, security issues have returned to the world's attention, and nontraditional security has gradually been taken seriously, especially in the Indo-Pacific region. With the resulting shift in the strategic focus of the U.S. towards the Indo-Pacific, the U.S. has continuously sent economic and military resources and international influence to the region. The Indo-Pacific region has gradually become the main battlefield for regional powers and world powers to demonstrate their strength and implement diplomatic strategies. Many new diplomatic relations and regional small multilateral cooperation have also emerged in the Indo-Pacific region. This multilateral cooperation covers economic, technological, military and other content. The characteristics and possible evolution paths of the “trilateral security mechanism” of the United States, Japan and South Korea will also inevitably bring new changes and possible conflicts to the region and even the world's foreign and defense.
Diagnósticos por Imagem: Contribuições Fundamentais na Saúde e Planejamento Clínico
Authors: Maria Lamego Rezende Adriana
Published: January 2025

O EBook Diagnósticos por Imagem: Contribuições Fundamentais na Saúde e Planejamento Clínico” explora o papel essencial da radiologia e das tecnologias de imagem na medicina moderna, destacando sua aplicação em diferentes áreas de saúde. Com uma abordagem prática e informativa, o livro aborda temas que conectam tecnologia, diagnóstico precoce e planejamento terapêutico, enfatizando como esses recursos transformam o cuidado com os pacientes.
Dividido em seis capítulos, o eBook discute a relevância do PACS (Sistema de Comunicação e Arquivamento de Imagens) na otimização dos serviços de radiologia, a importância da histerossalpingografia no planejamento familiar, e o impacto da biópsia prostática no diagnóstico precoce do câncer de próstata. Além disso, analisa a correlação clínica entre nódulos tireoidianos e tumores ovarianos, destacando a necessidade de uma abordagem multidisciplinar no manejo dessas condições.
O conteúdo também aborda os avanços tecnológicos nas imagens médicas, desde o uso de inteligência artificial até a integração de sistemas digitais, e como esses avanços guiam decisões terapêuticas personalizadas. Ao combinar estudos de caso, exemplos práticos e considerações éticas, o eBook oferece insights valiosos para profissionais de saúde e estudantes interessados em compreender a importância das imagens médicas no diagnóstico e no cuidado integral do paciente.

Este material é um guia indispensável para médicos, radiologistas, gestores hospitalares e todos os envolvidos na busca por excelência no atendimento clínico, apresentando como os diagnósticos por imagem se tornaram um pilar essencial na medicina contemporânea.
Gen Z's Interest in Online Travel Agencies in Indonesia: A Modified UTAUT2
Authors: Leon Jevin , Simanjuntak Fredian , Firmansyah Muhamad Dody
Published: January 2025

The rapid evolution of digital technology has transformed the travel and tourism industry, making Online Travel Agencies (OTAs) a preferred medium for trip planning and booking. OTAs offer seamless access to flights, accommodations, and other travel services, catering to Indonesia's mobile-first population and digitally savvy Gen Z users. This study examines factors influencing behavioral intention (BI) and use behavior (UB) toward OTAs, extending the UTAUT2 model with trust, website quality, and AI integration. Data from 300 respondents were analyzed using Structural Equation Modeling (SEM) via SPSS and AMOS. Results show that performance expectancy, effort expectancy, social influence, hedonic motivation, price value, trust, website quality, and AI integration positively affect BI. Habit negatively influenced BI, while BI, facilitating conditions, and habit positively impacted UB. These findings highlight the need for OTA providers to align user expectations with actual experiences, enhancing satisfaction and long-term adoption among Gen Z users.
El arte de enseñar cálculo: Estrategias innovadoras desde secundaria hasta la universidad
Authors: Armas Real Daniel Alberto , Chipantiza Salan Clara Marlene , Freire Freire Andrea Gabriela , Flores Mise Stalin Israel
Published: January 2025

El libro "El Arte de Enseñar Cálculo: Estrategias Innovadoras desde Secundaria hasta la Universidad" explora enfoques contemporáneos y creativos para la enseñanza del cálculo, adaptados a estudiantes de diferentes niveles educativos. Ofrece estrategias pedagógicas que fomentan el aprendizaje activo, la comprensión profunda de conceptos matemáticos y la resolución de problemas aplicados. El texto combina teoría y práctica, integrando tecnologías emergentes, metodologías dinámicas y ejemplos prácticos para fortalecer el interés y la participación de los estudiantes en el aprendizaje del cálculo. Además, incluye recomendaciones para docentes que buscan optimizar la transición entre los niveles educativo, promoviendo una educación matemática accesible y efectiva.
Study on the implementation effect of river and lake water environment management in Jingdezhen city
Authors: Zheng Xiaoling , Wu Yuting , Yang Lu
Published: January 2025

In recent years, the ecological protection of river and lake environments has gradually gained attention from both the government and society. To achieve a healthy aquatic ecosystem, our country places significant emphasis on safeguarding river and lake environments. The long river system is managed through a responsibility framework that involves party and government leaders, focusing on addressing pollution and ecological damage in these areas. Since the nationwide implementation of the river chief system in 2016, central and local governments have introduced a series of policies aimed at protecting water environments in rivers and lakes. At the end of 2016, the General Office of the Central Committee of the Communist Party of China issued guidelines on the comprehensive implementation of river management. These guidelines prioritize water conservation, spatial balance, systematic management, and the protection of water resources. The main tasks include improving water quality, restoring aquatic ecosystems, and actively exploring a management mechanism that establishes clear responsibilities, ensures coordination, enforces strict supervision, and provides robust protection for lake management. This framework aims to sustain the health of lakes and ensure their functions are utilized sustainably.
Détermination des exigences des sols du Mali pour la réalisation des blocs de terre stabilisée (BTS)
Authors:   Ecole normale d’Enseignement Technique et Professionnel (ENTP), Bamako, Mali, DAOU Yacouba , DAOU Esaii ,   Université de Ségou (US), Mali, TOUNKARA Yaya ,   Ecole normale d’Enseignement Technique et Professionnel (ENTP), Bamako, Mali, DOUMBIA Abdoulaye ,   Ecole doctorale des Sciences et Technologie du Mali (EDSTM), USTTB, Bamako, Mali
Published: January 2025

This research work aims to determine the requirements of stabilized earth blocks for their
effective use in Malian conditions.
Indeed, in order to enhance the value of local materials and contribute to reducing
construction costs in Mali, earth stabilization studies are essential. The subject of study
focuses on determining the requirements for stabilizing raw earth with CPJ 45 Portland
cement in order to obtain stabilized earth blocks (or bricks) (BTS) which constitute a very
promising technique among the various possible uses of earth and cement materials.
Raw earth used in construction in Mali for thousands of years, has mechanical and physicochemical properties that can be improved. In this document, a study analyzing the impact of
cement and water dosage on different BTS specimens at different curing conditions was
conducted in order to improve the mechanical characteristics of BTS.
To do this, we used a laterite from Sénou and located at the National Center for Research and
Experimentation in Building and Public Works (CNREX-BTP) whose grain size is located in
the spindle of the recommended earth diagram, and whose plasticity is located in the spindle
of the recommended earth diagram. In this context, different percentages of cement (4%, 6%,
8%, 10%), and water content (Wopt - 2%, Wopt, Wopt + 2%), were used to make BTS
specimens in order to see the variations in compressive strengths and tensile strengths by
splitting at 7 and 28 days of curing.
The compressive and tensile strengths increase as the cement percentage increases. The
variation in the optimum water content of ±2% influences the compressive and tensile
strengths, but these strengths are maximum at 15.10% water dosage which corresponds to the
optimum water content.
Stabilization with cement generally depends on the granulometry and plasticity of the earth
used, the cement dosage, the water content, and the curing of the blocks.
The tests described in this document were carried out at the National Center for Research and
Experimentation in Buildings and Public Works (CNREX-BTP), in Bamako.
Keyswords : Stabilized earth block, Stabilization, Material characteristics, Resistance,
Compression, Traction.
Escassez de equipamentos de proteção individual e o enfrentamento à COVID-19 pela enfermagem oncológica paliativa
Authors: Santiago Fernanda Barcellos , Silva Ana Lucia Abrahão da
Published: January 2025

Discutir as condutas utilizadas pela equipe de enfermagem quanto ao manejo dos Equipamentos de Proteção Individual (EPI) que se encontrava escasso frente à assistência de enfermagem a pacientes em tratamento paliativo oncológico acometidos pela Covid-19 através da educação permanente. Estudo descritivo de abordagem qualitativa. Utilizou-se o Discurso do Sujeito Coletivo para análise do material ancorado pela psicodinâmica do trabalho de Dejours. Estruturados em três ideias centrais: A) Sobre a escassez da máscara N95; B) Relações interpessoais; e C) Necessidade de escuta dos profissionais. Pela natureza dos dados apresentados, realizamos a discussão sob a ótica da psicodinâmica do trabalho por responder de forma mais adequada os dados obtidos. Dúvidas sobre o uso de EPI foram sanados, produção de condutas alinhadas, ambiente organizado. Foi estabelecido um canal de escuta com reuniões frequentes entre a chefia e a equipe de enfermagem.
Desfechos maternos e neonatais em gestantes com SRAG por COVID-19 no Estado do Rio de Janeiro: Uma avaliação do tipo de parto
Authors: Velasque Luciane , Motta Alana Stéphanie Esteves Villar da , Adesse Leila , Mendonça da Rocha Maíra , Peixoto Eduardo Mesquita , Carvalho Paula Rita Dias de Brito de , Carvalho Silvia Cristina de
Published: January 2025

A literatura relata que gestantes com diagnóstico de COVID-19 são menos propensas a ter um parto vaginal, e que aproximadamente dois terços do parto em mulheres com COVID-19 são cesarianas. Objetiva-se comparar o tipo de parto nos desfechos perinatais de gestantes que tiveram diagnóstico de SRAG por COVID-19 durante a gestação no Estado do Rio de Janeiro. Trata-se de um estudo transversal de base secundária, com linkage não-probabilístico dos bancos SIVEP-Gripe e SINASC de todos os casos notificados no Estado do Rio de Janeiro até janeiro de 2023. Foram analisados 239 partos vaginais e 728 cesarianas. A cesariana estava relacionada a piores desfechos em todas as variáveis estudadas, como maior necessidade de UTI e mortalidade materna, prematuridade, menor Apgar no 1º e 5º minuto e menor peso do recém-nascido quando comparados com o parto vaginal. A infecção pela COVID-19, por si só, não deve ser uma indicação para a cesariana, pois além de não haver evidências de que melhore as condições de nascimento, ela está associada a piores desfechos perinatais. Embora uma cesariana possa ser uma cirurgia que salva-vidas, ela pode colocar mulheres e bebês em risco desnecessário de problemas de saúde quando realizada sem necessidade clínica.
"Uma nova visão do trabalho e de nós mesmos"
Authors: Rodrigues Claudia dos Santos , Schmid Patrícia Cavalcanti , Pinto Carlos  José Moreno , Carvalho Zulmira Gomes Costa de , Gomes Rachel Novaes , Noronha Gabriel Vieira
Published: January 2025

O presente artigo expõe a experiência da equipe do Núcleo de Educação Permanente em Saúde da Fundação Estatal de Saúde de Maricá, com a realização da "Capacitação Acolhimento e Linhas de Cuidado", no período de 18/10/22 até 01/06/2023, a partir de uma demanda identificada em levantamento realizado junto aos profissionais das Unidades de Saúde da Família de Maricá. A referida capacitação abarcou 310 profissionais, dentre Agentes Comunitários de Saúde e Auxiliares Administrativos, havendo uma adesão de 97,4% dos participantes, chegando ao final do processo 299 deles. Os temas discutidos de forma interativa e articulados com os princípios da Educação Permanente em Saúde versaram sobre, acolhimento de acordo com a Política Nacional de Humanização do SUS, escuta qualificada, Comunicação Não Violenta em Saúde e linhas de cuidado. Como resultados, verificamos efeitos de ressignificação das práticas, reflexão crítica sobre os impasses nos processos de trabalho e na percepção dos profissionais capacitados sobre suas funções profissionais e seus modos de comunicação.
Examining the Role of Repo Rate in Controlling Inflation: Analyzing the Dynamics of the Proportional Relationship
Authors: Punno Mashrura Meshkat , Rafi Md Golam Mubasshir
Published: January 2025

A research study has assessed how effective repo rate adjustments as an instrument of monetary policy control across most economies could result in inflation. Based on raw data and reports collated from Bangladesh and India as well as global experiences between 2020 and 2022, the paper has shown that repo-linked measures were found to be deficient in containing structurally inefficient supply chains responsible for causing inflation. It also found that during the period 2020 to 2022, global supply chain issues, notably shortages in semiconductors and crises in energy, made up for about 50 to 60 percent of inflationary pressures. Repo rates increased during the period but, as the forgoing displays, economic recovery was hampered and inflation by those factors was not addressed. Studies across India and Bangladesh show repo measures, as well, were ineffective in dealing with inflation brought on by structure. Of interest, though, is Sri Lanka, where the highlight was on the need to have both monetary and fiscal interventions. The study proposes a multi-pronged approach aimed at controlling inflation with particular effects and focusing on investment in supply chains as well as fiscal measures and structural reforms.
SICKO: Systematic Imaging of Caenorhabditis Killing Organisms
Authors: Espejo Luis S , Freitas Samuel , Hofschneider Vanessa , Chang Leah , Antenor Angelo , Balsa Jonah , Haskins Anne , DeNicola Destiny , Dang Hope , Hamming Sage , Kelser Delaney , Sutphin George L
Published: January 2025

Caenorhabditis elegans
are an important model system for research on host-microbe interaction. Their rapid life cycle, short lifespan, and transparent body structure allow simple quantification of microbial load and the influence of microbial exposure on host survival.
C. elegans
host-microbe interaction studies typically examine group survival and infection severity at fixed timepoints. Here we present an imaging pipeline, Systematic Imaging of
Caenorhabditis
Killing Organisms (SICKO), that allows longitudinal characterization of microbes colonizing isolated
C. elegans
, enabling dynamic tracking of tissue colonization and host survival in the same animals. Using SICKO, we show that
Escherichia coli
or
Pseudomonas aeruginosa
gut colonization dramatically shortens
C. elegans
lifespan and that immunodeficient animals lacking
pmk-1
are more susceptible to colonization but display similar colony growth relative to wild type. SICKO opens new avenues for detailed research into bacterial pathogenesis, the benefits of probiotics, and the role of the microbiome in host health.
Human genetic ancestry, Mycobacterium tuberculosis diversity and tuberculosis disease severity in Dar es Salaam, Tanzania
Authors: Zwyer Michaela , Xu Zhi Ming , Ross Amanda , Hella Jerry , Sasamalo Mohamed , Rotival Maxime , Hiza Hellen , Rutaihwa Liliana K , Borrell Sonia , Reither Klaus , Fellay Jacques , Portevin Damien , Quintana-Murci Lluis , Gagneux Sebastien , Brites Daniela
Published: January 2025

Infectious diseases have affected humanity for millennia and are among the strongest selective forces. Tuberculosis (TB) is an ancient disease, caused by the human-adapted members of the
Mycobacterium tuberculosis
complex (MTBC). The outcome of TB infection and disease is highly variable, and co-evolution between human populations and their MTBC strains may account for some of this variability. Particular human genetic ancestries have been associated with higher susceptibility to TB, but socio-demographic aspects of the disease can confound such associations. Here, we studied 1,000 TB patients from Dar es Salaam, Tanzania, together with their respective MTBC isolates, by combining human and bacterial genomics with clinical data. We found that the genetic background of the TB patient population was strongly influenced by the Bantu migrations from West Africa, which is in contrast to the corresponding MTBC genotypes that were mainly introduced from outside Africa. These findings suggest a recent evolutionary history of co-existence between the human and MTBC populations in Dar es Salaam. We detected no evidence of an effect of human genetic ancestry, or MTBC phylogenetic diversity alone, nor their interaction, on TB disease severity. Treatment-seeking, social and environmental factors are likely to be the main determinants of disease severity at the point of care in this patient population.
The eduWOSM: a benchtop advanced microscope for education and research
Authors: Carter Nicholas J , Martin Douglas S , Molloy Justin E , Cross Robert A
Published: January 2025

To improve access to advanced optical microscopy in educational and resource-limited settings we have developed the eduWOSM (
edu
cational
W
arwick
O
pen
S
ource
Mic
roscope), an open hardware platform for transmitted-light and epifluorescence imaging in up to 4 colours, including single molecule imaging. EduWOSMs are robust, bright, compact, portable and ultra-stable. They are controlled entirely by open source hardware and software, with an option for remote control from a webpage. Here we describe the core eduWOSM technology and benchmark its performance using 3 example projects, single fluorophore tracking of tubulin heterodimers within gliding microtubules, 4D (deconvolution) imaging/tracking of chromosome motions in dividing human cells, and automated single particle tracking in vitro and in live cells with classification into subdiffusive, diffusive and superdiffusive motion.
Aminoglycoside tolerance in Vibrio cholerae engages translational reprogramming associated with queuosine tRNA modification
Authors: Fruchard Louna , Babosan Anamaria , Carvalho Andre , Lang Manon , Li Blaise , Duchateau Magalie , Giai Gianetto Quentin , Matondo Mariette , Bonhomme Frederic , Hatin Isabelle , Arbes Hugo , Fabret Céline , Corler Enora , Sanchez Guillaume , Marchand Virginie , Motorin Yuri , Namy Olivier , de Crécy-Lagard Valérie , Mazel Didier , Baharoglu Zeynep
Published: January 2025

Tgt is the enzyme modifying the guanine (G) in tRNAs with GUN anticodon to queuosine (Q). tgt is required for optimal growth of Vibrio cholerae in the presence of sub-lethal aminoglycoside concentrations. We further explored here the role of the Q34 in the efficiency of codon decoding upon tobramycin exposure. We characterized its impact on the overall bacterial proteome, and elucidated the molecular mechanisms underlying the effects of Q34 modification in antibiotic translational stress response. Using molecular reporters, we showed that Q34 impacts the efficiency of decoding at tyrosine TAT and TAC codons. Proteomics analyses revealed that the anti-SoxR factor RsxA is better translated in the absence of tgt. RsxA displays a codon bias toward tyrosine TAT and overabundance of RsxA leads to decreased expression of genes belonging to SoxR oxidative stress regulon. We also identified conditions that regulate tgt expression. We propose that regulation of Q34 modification in response to environmental cues leads to translational reprogramming of transcripts bearing a biased tyrosine codon usage. In silico analysis further identified candidate genes which could be subject to such translational regulation, among which DNA repair factors. Such transcripts, fitting the definition of modification tunable transcripts, are central in the bacterial response to antibiotics.
Ocular biomarker profiling after complement factor I gene therapy in geographic atrophy secondary to age-related macular degeneration
Authors: Hallam Thomas M , Gardenal Emanuela , McBlane Fraser , Cho GaEun , Ferraro Lucy Lee , Pekle Eva , Lu Darlene , Carney Kate , Wenden Claire , Beadsmoore Hannah , Kaiser Sergio , Drage Lauren , Haye Thomas , Kassem Iris , Rangaswamy Nalini , Obeidat Ma’en , Grosskreutz Cyndy , Saint-Geniez Magali , Steel David H , MacLaren Robert E , Ellis Scott , Harris Claire L , Poor Stephen , Jones Amy V
Published: January 2025

Complement biomarker analysis in ocular fluid samples from subjects with geographic atrophy (GA) in a Phase I/II clinical trial of subretinal AAV2 complement factor I (
CFI
; FI) gene therapy, PPY988 (formerly GT005), to understand target pharmacokinetics/pharmacodynamics. Clinical findings were subsequently utilized to investigate the therapeutic dose in an
in vitro
complement activation assay.Biomarker data were evaluated from 28 subjects in FOCUS, a Phase I/II clinical trial evaluating the safety and efficacy of three ascending doses of PPY988.Vitreous humor (VH), and aqueous humor (AH) from subjects before surgery and at serial timepoints (week 5 or 12, 36, 96) were evaluated for changes in levels of intact complement factors I, B and H (FI, FB, FH) components C3, C4, and C1q and breakdown products (Ba, C3a, C3b/iC3b, C4b) using validated assays and OLINK
®
proteomics.A modified
in vitro
assay of complement activation modelling VH complement concentrations was used to compare PPY988 potency to the approved intravitreal C3 inhibitor pegcetacoplan (Apellis) and complement Factor H (FH).An average 2-fold increase in VH FI was observed post-treatment at week 36 and week 96. This correlated with a marked post-treatment reduction in VH concentration of the FB breakdown product Ba and Ba:FB ratio, but minimal changes in C3a and C3b/iC3b levels. Variable concordance in complement biomarker levels in VH versus AH suggest AH is not a reliable proxy for VH for complement activation. During the experimental comparison of doses, a 2-fold increase of FI achieved in the vitreous had only a minor effect on the complement amplification loop
in vitro
, indicating limited impact [IC50: 1229nM]. Pegcetacoplan completely blocks C3a generation at concentrations much lower than the estimated trough level for monthly intravitreal injections [IC50: 2nM]. Supplementation with FH in the assay revealed similar potency to pegcetacoplan [IC50: 6nM].PPY988 subretinal gene therapy may not have provided sufficient FI protein to meaningfully modulate complement activation to slow GA growth. Reviewing VH biomarkers is important for understanding target expression, pathway engagement, and determining optimal dose, thereby informing future clinical development.
GAN inversion and shifting: recommending product modifications to sellers for better user preference
Authors: Kumar Satyadwyoom , Sharma Abhijith , Narayan Apurva
Published: January 2025

In efforts to better accommodate users, numerous researchers have endeavored to model customer behavior, seeking to comprehend how they interact with diverse items within online platforms. This exploration has given rise to recommendation systems, which utilize customer similarity with other customers or customer-item interactions to suggest new items based on the existing item catalog. Since these systems primarily focus on enhancing customer experiences, they overlook providing insights to sellers that could help refine the aesthetics of their items and increase their customer coverage. In this study, we go beyond customer recommendations to propose a novel approach: suggesting aesthetic feedback to sellers in the form of refined item images informed by customer-item interactions learned by a recommender system from multiple consumers. These images could serve as guidance for sellers to adapt existing items to meet the dynamic preferences of multiple users simultaneously. To evaluate the effectiveness of our method, we design experiments showcasing how changing the number of consumers and the class of item image used affect the change in preference score. Through these experiments, we found that our methodology outperforms previous approaches by generating distinct, realistic images with user preference higher by 16.7%, thus bridging the gap between customer-centric recommendations and seller-oriented feedback.
Antibacterial Activity of Ethanol Extract of Gotu Kola (Centella asiatica L.) Leaves against Pseudomonas solanacearum: Phytochemical Study and Bioactivity Testing
Authors: Syahfari Helda , Saputra Rifki Ihsan , Azham Zikri , Sitorus Saibun
Published: January 2025

Pseudomonas solanacearum bacteria can cause wilt disease in tomatoes, chilies, tobacco, potatoes, and Solanaceae plants in general. The use of synthetic pesticides to treat wilt disease can cause negative impacts on plants, humans, and the environment, so it is necessary to find environmentally friendly pesticides.  This research aims to determine the compounds contained in gotu kola (Centella asiatica L.) leaf extract, and the antibacterial effect of gotu kola leaf extract against the bacteria Pseudomonas solanacearum. The research was carried out at the Wood Properties and Product Analysis Laboratory, Department of Agricultural Agrotechnology, Samarinda State Agricultural Polytechnic, Keledang River, Samarinda Seberang District, Samarinda City, East Kalimantan from January to March 2023.  Phytochemical test of gotu kola leaf extract to determine the content of secondary metabolite compounds. Consists of tannins, flavonoids, saponins, alkaloids, and steroids. To test the antimicrobial (anti-bacterial) activity, several concentrations of gotu kola leaf extract were carried out, namely 2.5% (25 ml/l); 5% (50 ml/l), 10% (100 ml/l); 15% (150 ml/l) and 20% (200 ml/l). The research results showed that the crude ethanol extract of gotu kola leaves contains secondary metabolites such as tannins, flavonoids, saponins, alkaloids, steroids. The ethanol extract of gotu kola leaves with a concentration of 50 ml/l (5%) showed more effective antibiotic activity compared to higher concentrations, namely 10% (100 ml/l), 15% (150 ml/l), and 20% (200 ml/l).
Prevalence of Dihydropyridine Dehydrogenase (DPD) Deficiency in Cancer Patients in Senegal
Authors: A Ndiaye , C Narzoz , A Samba , S Thiam , IY Soumah , F Diedhiou , NF Coly , F Cissé , F Diallo
Published: January 2025

Introduction: Cancers are a real public health problem. Their prevalence varies greatly among the world population and many therapeutic strategies, such as chemotherapy, have been put in place and most chemotherapy protocols are based on products containing fluoropyrimidines. However, the use of the suggested products can cause side effects in patients who are deficient in dihydropyrimidine dehydrogenase enzyme, which plays an important role in the metabolism of fluoropyrimidine products. Our study was conducted in this context to determine the activity of this enzyme in a population with cancer.
Methods: This is a prospective study whose sample collection took place during the months of July and August 2021. The study involved 103 cancer patients were selected following a questionnaire and a consultation of the chemotherapy protocol. Blood samples were taken on EDTA tubes and uracil (U) and dihydrouracil (UH2) were measured using HPLC.
Results: In our study population, the mean age is 49.77 years ±2.46 years. And women were the most affected, representing 83% of patients and a sex ratio of 0.19. 47% of women had breast cancer, 25% had cervical cancer, 6% had ovarian cancer, 3% had rectal cancer, and 1% had other types of cancers such as vulvar, bladder, stomach, pelvic colon, chest, throat, and cheek/eye and cavirum. For the UH2/U ratio, the mean value was 12.6 ± 0.48 with a standard deviation of 4.91 ± 0.95. DPD activity was normal in 33% of our patients, while 66.9% had a partial deficit and 0.1% had a total deficit. The 40 to 50 years and 50 to 60 age groups had high deficiency in this enzyme (21.3% and 20.3% respectively), with a total deficiency observed specifically in the 40 to 50 age group.
Conclusion: The results of our study highlighted the importance of screening for DPD deficiency prior to fluoropyrimidine administration. Screening makes it possible to identify patients at risk and to adapt chemotherapy doses, accordingly, thus reducing the incidence of severe and potentially fatal toxicities.
Optimizing Business Intelligence for Nigerian Small and Medium Scale Enterprises: The Influence of Economics Education and Guidance Counseling on Decision-Making and Growth
Authors: Romanus Nduji , Benedict BAHAGO, Samaila , Wokji GOTIP, Nehemiah , Chris Oriaku
Published: January 2025

The problem of SMEs today is compounded by the rapid pace of technological advancements in Nigeria. Addressing the challenge is essential for maximizing the potential of Business Intelligence as a strategic tool for enhancing decision-making and driving organizational success. The study examined optimizing business intelligence for Nigerian small and medium scale enterprises: the influence of economics education and guidance counseling on decision-making and growth. The study applied cross-sectional survey research design. The population of the study comprised selected 1,331 SMEs in Nigeria. The sample size of 302 was obtained using Krejcie and Morgan (1970) formula. The research adopted stratified random sampling techniques. Data were collected using structured questionnaire. The descriptive statistics, such as mean and standard deviation were used to analyze the data and the hypotheses were tested using t-test. The finding has it that data sources, data warehousing and reporting tools have effect on performance of SMEs in Nigeria. The research concluded that if the wrong Business Intelligence is implemented without good research and planning, it could be a failure initiative. The study recommended  that  Government should organized free workshops on economic principles relevant to SMEs and create counseling centers or platforms where SME owners can receive personalized advice on decision-making, growth strategies, and overcoming operational challenges.
12 years of Male Breast Cancer Experience at One Center
Authors: Çarkit Sedat , Oz Bahadir , Gok Mustafa , Akcan Alper Celal , Karaagac Mustafa , Aslan Dicle , Mutlu Emel , Solak Ismail , Sozuer Erdogan Mutevelli
Published: January 2025

Background

Male breast cancer is a sporadic disease and only one in every 100 new breast cancer patients is male. There are few satisfactory clinical studies on male breast cancers in the literature. We aim to share the clinicopathologic and demographic characteristics of male breast cancer patients admitted to our clinic in the last 12 years and our experience in the treatment of these patients.

Methods

The data of patients who were referred to our clinic with a diagnosis of breast cancer, suspicion of breast cancer, or who presented to our outpatient clinic with symptoms of breast discharge, palpable mass in the breast and were diagnosed with breast malignancy or suspicion of malignancy between 2010 and 2022 were retrospectively evaluated from the data bank records of our hospital. 28 patients were included in the study. Patients' clinical and pathologic data, treatment options, approach to the axilla, pathology results, and survival were evaluated.

Results

There were 28 patients in the research. 22 had mastectomies. No surgical intervention was carried out for the five patients who had metastases. One of these patients died during follow-up due to advanced comorbidities and metastatic disease. Another patient declined surgery following neoadjuvant chemotherapy and passed away during follow-up (ex). The third patient passed away during neoadjuvant chemotherapy, and two patients are still undergoing neoadjuvant treatment.

Conclusion

Male breast cancers are rare diseases. RT after mastectomy increases survival in male patients as well as in female patients. However, RT in male patients is not standardized today. In our study, 15 patients received adjuvant RT.
Geotermia para Fins de Resfriamento: uma prospecção patentária
Authors: Ferreira Antonio Cardoso , Durante Luciane Cleonice , Callejas Ivan Julio Apolonio , Vera Lucas Ferrari
Published: January 2025

Este artigo trata de uma prospecção patentária acerca do tema de geotermia, que se propõe a utilizar o solo como forma de se trocar calor com o ar. Em consequência da sua alta capacidade térmica, o solo pode trabalhar como um reservatório térmico, aquecendo ou resfriando o ar do ambiente interno das edificações, dependendo das condições climáticas. Assim, o objetivo foi mapear tecnologias relacionadas à geotermia aplicadas ao resfriamento. Utiliza-se a revisão patentária por meio da plataforma Questel Orbit®, tendo sido identificadas 226 patentes. Os resultados evidenciam que entre os maiores cessionários está a Tokai Kogyo, fábrica japonesa com sede em Õnu, que se sobressai dos outros cessionários, com 15 famílias de patentes, e as tecnologias se relacionam a máquinas e equipamentos do setor de climatização. A pesquisa reconhece os benefícios econômicos e ambientais da geotermia em comparação com soluções tradicionais, muito embora somente um único estudo tenha sido localizado para climas tropicais.
Mapeamento e Caracterização das Empresas que Comercializam Fitoterápicos Industrializados e Manipulados no Brasil
Authors: Mafra Rosana Zau , Medeiros Rafael Lima , Andrade Gabriela Ramos , Nascimento Nadja Maria Lepsch da Cunha
Published: January 2025

Esta pesquisa foi motivada pela necessidade de se conhecer como os fitoterápicos são inseridos no mercado nacional, investigando a participação dos atores da Região Norte e a inclusão das espécies medicinais amazônicas. O estudo partiu da seleção de 33 espécies nativas com distribuição geográfica pelo Estado do Amazonas. Em seguida, foram levantados os produtos disponíveis no mercado nacional derivados dessas plantas, elencados em duas categorias: 1. fitoterápicos industrializados regulados pela Anvisa; e 2. fitoterápicos manipulados por farmácias magistrais e suplementos regulados por um conjunto de legislações não farmacêuticas, comercializadas no mercado virtual. Vinte variáveis foram subdivididas em quatro grupos: dados comerciais; composição; quantidades; e farmacologia. Dos 153 produtos identificados, 94,7% pertencem à categoria 2, sendo o guaraná (Paullinia cupana) a espécie com o maior número de produtos industrializados e manipulados/suplementos recuperados. A distribuição geográfica das empresas está concentrada na Região Sudeste do Brasil, corroborando análises anteriores publicadas. A presença de produtos irregulares no mercado on-line é possivelmente muito alta.
Uso do MDMA (Ecstasy) em Sessões de Psicoterapia para o Tratamento do TEPT Crônico: um estudo bibliométrico
Authors: Viana Rachel Lais Brandão , Conceição Maria Hosana
Published: January 2025

Este artigo tem como objetivo realizar uma revisão bibliográfica a respeito da utilização clínica do 3,4-Metilenodioximetanfetamina (MDMA ou Ecstasy) em sessões de psicoterapia do Tratamento do Transtorno de Estresse Pós-Traumático (TEPT). Esse distúrbio é comumente tratado com antidepressivos, que atuam sobre neurotransmissores, além do acompanhamento psicoterapêutico, no entanto, alguns pacientes não reagem bem ao tratamento disponível, e o transtorno se torna crônico. Para a busca de artigos, foram usadas as bases de dados da PubMed e do Scielo. Para a prospecção de patentes e de suas tecnologias, foi consultada a plataforma Orbit Intelligence a partir das palavras-chave “MDMA” ou “3,4-Metilenodioxianfetamina”. Artigos mais recentes evidenciam que o MDMA é uma substância potencial para auxiliar a psicoterapia no tratamento do TEPT. Além disso, as tecnologias mais associadas às inovações com o MDMA estão presentes nas patentes, por exemplo, o Pedido de Patente n. “JP2023549405”, um pró-fármaco do MDMA.
Inovação Social por meio da Transferência de Conhecimento: estratégias, desafios e impactos
Authors: Pantaleão Elba de Oliveira , Zambra Elisandra Marisa
Published: January 2025

Objetiva-se com este estudo compreender o fenômeno da transferência de conhecimento e tecnologia no contexto da inovação social, destacando as estratégias-chave, os desafios enfrentados e os impactos observados. Trata-se de uma pesquisa exploratória e descritiva, realizada a partir de uma revisão integrativa dos estudos publicados na base Web of Science. Foram selecionados oito artigos que atendem ao objetivo da pesquisa. A maioria dos artigos analisados apresenta estudos de caso em que Instituições de Ensino Superior desempenham um papel central, utilizando estratégias de envolvimento público por meio de projetos, programas, cursos e workshops. Entre os desafios enfrentados na transferência de conhecimento para promover a inovação social, estão a cultura universitária, as dificuldades de engajamento dos atores sociais, a estrutura burocrática e a mensuração do valor social. No entanto, os impactos positivos são diversos, incluindo a geração de valor social e econômico, por exemplo, a geração de renda e de empregos e a lucratividade nos negócios.