bluuebunny/crossref_metadata_2025 huggingface dataset:
Cephalometric studies of Ukrainian adolescents with orthognathic bite by the method of E.P. Harvold
Authors: Chernysh A. V.
Published: January 2019

According to many numerical literary sources, the modern direction of provision of dental care involves an individual approach to the patient, in particular, based on his gender, age and nationality. This calls for the creation of its own regulatory bases for the population of different countries and ethnic groups, taking into account all possible criteria. The purpose of the study is to establish and analyze the cephalometric parameters by the method of E.P. Harvold in young men and women of Podillia region of Ukraine with orthognathic bite. Primary lateral teleroentgenograms of 38 young men and 55 women with normal occlusion close to orthognathic bite, obtained using the Veraviewepocs 3D device, Morita (Japan), were taken from the data bank of the research center of the National Pirogov Memorial Medical University, Vinnytsya. Cephalometric measurements were performed according to the recommendations of E.P. Harvold. Statistical processing of the obtained results was carried out in the license package "Statistica 6.0" using nonparametric methods of estimating the results. When comparing teleroentgenographic indices between boys and girls of the Podillia region of Ukraine with orthognathic occlusion, significantly higher values of the length of the upper and lower jaw, lower facial height and interjaw differences in young men were established, and Ap1uAp1l-DOP angle was significantly higher in young women. When comparing these data with the results obtained by E.P. Harvold, the boys and girls of the Podillia region of Ukraine with orthognathic bite have significantly lower values of the length of the upper and lower jaw and lower facial height. In addition, Ukrainian young women have significantly higher values of the Ap1uAp1l-DOP angle and a tendency to lower interjaw differences, while in young men, the tendency towards higher values of the cross-sectional angle than the value of these parameters obtained by E. Harvold is established. The results of the study confirm the need to create a normative basis for teleroentgenographic indices by the method of E.P. Harvold for the population of different regions of Ukraine, taking into account both sexual and age affiliation.
The peculiarity of appendectomy of patients with high risk of cardiopulmonary insufficiency
Authors: Zaporozhchenko B.S. , Yahya Hasan , Borodaev I.I. , Kachanov V.N. , Vasyliev O.A.
Published: January 2019

Acute appendicitis (AA) ranks first in the frequency of emergence of urgent surgical diseases in hospitals in Ukraine. In recent years, laparoscopic appendectomy has become one of the “gold standards” of surgery. Of particular interest in this group of patients is a gasless and with small amount of gas laparoscopy, in which the endosurgical space in the abdominal cavity is created with the help of endolifts, in which the abdominal wall is raised mechanically, without creating a pneumoperitoneum. Objective — to determine the feasibility and effectiveness of the use of various techniques for laparoscopic appendectomy of patients with a high risk of cardiopulmonary insufficiency. All patients with АA were divided into 2 groups: Group 1 — 72 patients with АA diagnosis, with a high risk of cardiopulmonary failure, who underwent open appendectomy; Group 2 — 85 patients with АA diagnosis with a high risk of cardiopulmonary failure, who underwent laparoscopic appendectomy with pneumoperitoneum and using laparolifting method of patients. The laparolifting was performed with the help of the device developed by the clinic for laparolifting on Zaporozhchenko-Kolodii. When analyzing the results of treatment of patients with IІ group, it should be noted that laparoscopic appendectomy, especially with laparolifting, may be the operation of choice in the treatment of patients with acute appendicitis. This technique is absolutely safe, reliable and affordable in the implementation of most surgeons. So, laparoscopic appendectomy can be performed in virtually all patients with acute appendicitis with high risk of cardio-pulmonary insufficiency with the avoidance of postoperative complications.
Experience of using polymer clips in patients with acute calculous cholecystitis in combination with chronic viral hepatitis
Authors: Kashtalian M.A. , Kolotvіn A.O. , Kolotvina L.I. , Kvasnevskiy A.A.
Published: January 2019

In the article, the authors summarize the materials presented in the scientific foreign and domestic literature and acquired their own experience in the use of polymer and metal clips in patients with acute calculous cholecystitis (GKH) in combination with chronic viral hepatitis (CWG). The aim of the work was to optimize the clipping of the cystic duct during laparoscopic cholecystectomy in patients with acute calculous cholecystitis and chronic viral hepatitis. In the period from 2015 to 2017, 822 patients with GCS performed laparoscopic cholecystectomy (LHE). Clinching of the bladder duct and the bladder artery was carried out with metal clips in 339 (41.2%) patients and polymer clips in the type “Hem-o-lock” in 483 (58.8%) patients. In the study group, 59 (7.1%) patients with CKD in combination with CKD were included, in which for clumping of the bladder duct and bladder artery in 17 (28.8%) cases metal clips were used and in 42 (71.2%) cases polymeric. “Slipping” of clips from the clipper among the patients, in whom clapping was done with metal clips, was in 58 (17.1%) patients without CKD, and in 5 (29.4%) patients with CKD with CWG. When clumping with polymer clips, “slipping” clips from the applier was in 17 (3.5%) patients without HV and in 1 (2.4%) patients with HVC. Thus, polymer clips of the Hem-o-lock type are designed for use with an appropriate clipper, which fully repeats the structure of the clip, the problem of “slipping” in patients with GCS during the clumping of the bladder duct and bladder arteries can minimize and prevent the occurrence of complications such as bile duct and bleeding.
Video-assisted thoracoscopy in the treatment of patients with acute purulent mediastinitis
Authors: Snizhko S.S.
Published: January 2019

Treatment of patients with acute purulent mediastinitis (APM) is one of the most complex problems of modern surgery. Mortality in patients with APM can reach 17–80%. The aim of the work was to improve the results of treatment of patients with acute purulent mediastinitis using video-assisted thoracoscopy (VATS). During 2000–2018, 87 patients with APM were treated at the thoracic department of the Ivano-Frankivsk Regional Clinical Hospital. The main methods of surgical treatment in primary APM were lateral thoracotomy, mediastinotomy, suturing of the perforation opening of the esophagus with active drainage of the mediastinum and pleural cavity. The VATS with perforations of the esophagus (EP) with APM was applied in 9 (29.1%) patients and with secondary APM in 8 (13.8%) patients. The advantages of the VATS are minimal operational trauma, reducing the time of operation. According to the diagnosis of the EP, complicated APM, the minimally invasive surgical intervention in some cases can provide sufficient radicalism of intervention with minimal traumatism. The purpose of the VATS should be considered sanation and drainage of paraoesophageal fiber and fiber mediastinum. Thus, the choice of the method of surgical treatment of patients with acute purulent mediastinitis should be strictly individual in each case. Video-assisted thoracoscopy is a method of choice in surgical treatment of patients with acute purulent mediastinitis, which is a little traumatic method, provides a good visualization of all mediastinal units, allows for adequate detection and drainage of affected areas. Prospects for further research — improvement of methods of surgical treatment of patients with APM using the method of the VATS.
Pemikiran Pendidikan Islam: Konseptualisasi Pendidikan Karakter Dalam Perspektif Intelektual Islam Klasik
Authors: Wiyono Dwi Fitri
Published: January 2019

Character education occupies a strategic position in Islamic education in Indonesia. its urgency is felt to be conceptually formulated in an Islamic perspective. The study of character education in the thinking of classical to modern Islamic figures has always been an important theme to be studied and developed in the treasury of Islamic education. The underlying background includes: First; character education is an important theme that is of concern to policy makers in formulating educational goals. second; Character education is the main basis for the idea of Islamic education. Third; the concept and thought of character education initiated by classical Islamic intellectuals have similarities and differences with the concept of education originating from the west and the concept of inherited character education from ancient Greece and Arabia.
О типологических особенностях текста Евангелия Тип-7 из собрания РГАДА ф. 381, № 7
Authors: Szwed Zofia
Published: January 2019

The article is devoted to the 13th century Gospel Book, which is kept in the Russian State Archive of Old Records and registered under reference F. 381, № 7. The aim of the paper is to define the similarity of the manuscript to other old texts according to its content and order of readings. Moreover, the author attempts to find the Gospel Book’s place in the classification of full lectionaries. In the course of the analysis it is stated that the text has various typological features. Despite the fact that in some elements it resembles the South Slavonic Miroslav Gospel, it belongs to the most common type of full lectionaries in Ruthenia among which the leading role is played by Mstislav Gospel. Furthermore, the manuscript reflects the peculiar features of Milata Gospel, which makes it a part of the class of manuscripts represented by this old text of Novgorod.
Nadieżdy Teffi związki z Polską
Authors: Bielniak Nel
Published: January 2019

The contacts of the popular Russian humorist and the representative of the first wave of emigration with Poland begin at the level of family connections. Teffi used to visit Warsaw where she became acquainted with the representatives of the local literary world. Her works, translated into Polish, were published both by numerous magazines, separate collections and showed on stage. Polish themes appear in Teffi’s works too.
Dramaturgia Michaiła Bułhakowa na współczesnej polskiej scenie. Uwagi o spektaklu Molier. Z urojenia
Authors: Gracla Jadwiga
Published: January 2019

In this sketch, the author attempts to analyze the spectacle of the Bialystok group PAPAHEMA created on the basis of the drama Bulgakov Molier, or conspiracy of the saints. In the spectacle, the display of biographical threads for the purpose of universal aim`s has been dispensed with. According to the author, the creator of the spectacle, while remaining faithful to the assumptions of the text, created a universal spectacle, presenting motifs of human behavior and, above all, exposing the theatricality of the text.
Przegląd literatury ukraińskiej w przekładach na język polski po 1989 roku
Authors: Czetyrba-Piszczako Mirosława
Published: January 2019

The article aims at presenting an outline of the Ukrainian literature that has been popularised on the Polish publishing market after 1989. The transformation taking place in Ukraine encouraged Poles’ interest in their south-eastern neighbour. This popularity is not yet reflected in their acquaintance with Ukrainian literature published in Poland. This shows that Ukraine is still perceived through a political rather than literary or cultural perspective. The undertaken analysis revealed that the range of Ukrainian literature in translation offered by Polish publishing houses is not, however, as modest as it might be expected judging by its little popularity.
Ukraińskie przysłowia i porzekadła w polskim przekładzie Eneidy Iwana Kotlarewskiego
Authors: Czetyrba-Piszczako Mirosława
Published: January 2019

Ivan Kotlyarevsky’s Eneyida is a poem rich in paremic elements. The poem was considered a breakthrough in Ukrainian literature, not only because it was written in lively Ukrainian language, but, thanks to the author’s fascination with the folk culture and artistic traditions of previous eras, it also constituted a link between old and new Ukrainian literature. The aim of this article is to investigate the types of translation strategies used by Piotr Kupryś in the translation of Eneyida, especially those concerning paremic elements that are heavily influenced by cultural connotations. The analysis shows that Kotlarewski introduced existing proverbs, and also introduced new ones by processing others. In the Polish translation, Kupryś was not only obliged to preserve the content of the proverbs, but also to reproduce the plasticity of the images on which they were built. The techniques most widely used in his translation include domestication, exotication and tracing. It seems that the translator did manage to extract the meaning of the proverbs; he did not, however, always preserve their expressive and stylistic value, which was the basic element of building the satirical effect of the poem.
Prolegomena do tagоwania frazemów w równoległym korpusie rosyjsko-polskim (literatura piękna) w aspekcie przekładoznawczym
Authors: Fedorushkov Yury
Published: January 2019

This article considers tagging methods for parallel Russian-Polish phrasemathic objects. In particular, an opinion about the annotation tool brat v1.3.is given. This online tool offers a palette of possibilities for classifying words and phrases in parallel texts. Working with this software is largely simplified by a user-friendly interface, and therefore working with the corpus does not cause difficulties for philologists and translators who do not have programming skills. As an example of such a classification, the layout of the metadata system for tagging Russian and Polish parallel phrasemes is described. These resources allow experience to be gathered and concurrent objects to be categorized in the workshop of a translator. As an example, the article presents the tagging of Verb-Noun of the text classified as collocation phrasemes, for example, погасить свет. The status of Verb-Noun constructions is also discussed, which, according to a number of factors, relate to autonomous phrases, although with the status of “free compatibility”, for example, поехать в клуб. A number of recommendations is proposed for the configuration of parallel texts at the level of single sentences.
До питання про основні напрями вивчення емоцій у польському, російському та українському мовознавстві
Authors: Ніколаєнко Лариса
Published: January 2019

The article is devoted to the main directions of studying emotions in linguistics. The subject of the study are the works of Polish, Russian and Ukrainian linguists, devoted to the description of the verbalisation of emotions. The analysis of works shows that the main linguistic directions in the study of the concepts of emotions are linguocognitivistics and linguoculturology. The main approaches to the semantic description of the verbalisation of emotions can be called semantic, metaphorical and combined. Despite the diversity of vectors of linguistic analysis, each study reconstructs a separate fragment of the general linguistic picture of the world of emotions.
ASSESSMENT OF GOLD NANOPARTICLE EFFECT ON PROSTATE CANCER LNCaP CELLS
Authors: Reznikov A G , Salivonyk O A , Sotkis A G , Shuba Y M
Published: January 2019

In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and functional effect on tumor cells. Aims: This study was designed to describe the effect of non-functionalized AuNPs on LNCaP prostate cancer cells growth. Material and Methods: LNCaP cells were cultured in R PMI-1640 medium containing AuNPs covered by polyvinylpyrrolidone of average size 26.4 nm (10.0 μg/ml). Counts of cells were calculated and their morphology was examined. Results: AuNPs conglomerates have been visualized in cultured cells. After 4-day incubation in presence of AuNPs significant retardation of LNCaP cells growth was observed both in 5α-dihydrotestosterone stimulated and non-stimulated cultures. No morphological changes of live LNCaP cells were seen in any experiment. Conclusion: Given absence of morphological changes in live cells and dribble and relatively constant numbers of dead cells, it was concluded that inhibitory effect of AuNPs on LNCaP cells growth was caused by alterations of proliferation.
ISOLECTINS OF PHYTOHEMAGGLUTININ ARE ABLE TO INDUCE APOPTOSIS IN HEp-2 CARCINOMA CELLS IN VITRO
Authors: Kochubei T O , Maksymchuk O Y , Piven O O , Lukash L L
Published: January 2019

Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3.
PHARMACOLOGICAL EFFECT OF AMINOFERROCENE IN MICE WITH L1210 LEUKEMIA
Authors: Chekhun V F , Mokhir A , Daum S , Todor I , Lukianova N Yu , Shvets Yu V , Burlaka A P
Published: January 2019

Aim: To study the cytostatic and some biological effects of aminoferrocene using mice with L1210 lymphoid leukemia. Materials and Methods: Experiments were performed on BDF1 male mice (DBA/2, female × C57Bl/6, male) with transplantable L1210 lymphoid leukemia. Determination of antitumor activity of Benzyl-Fc Boron (Bn), it was injected intraperitoneally 6 times daily, starting on day 2 after L1210 leukemia cell transplantation. Doses of Bn such as 26; 260 and 2600 μg/kg were used. The determination of intracellular content of cardiolipin, thiols, reactive oxygen species (ROS) and also analysis of Annexin V positivity and mitochondrial transmembrane potential (JC-1 staining) were performed with use of flow cytometry. The levels of “free iron” complexes, transferrin active forms and the rate of NO generation were measured by EPR-specroscopy. Results: Six daily injections of Bn at a dose of 26 μg/kg resulted in an increased survival of mice with L1210 leukemia by 28% (p < 0.05). Bn led to an increase of apoptotic cells number and ROS amount in leukemia cells. Besides, Bn caused a decrease of cardiolipin and nonprotein thiol compounds content. The membrane electrochemical potential of cell mitochondria was decreased also after Bn administration. Studies using EPR-spectroscopy revealed a significant increase in a level of “free iron”, content of transferrin active species and generation rate of NO by inducible NO-synthase in L1210 cells after aminoferrocene administration. Conclusion: Our data indicate that Benzyl-Fc Boron can be promising candidate for realizing a new strategy of anticancer therapy with the use of ROS-inducing agents.
VALENTYN LEONIDOVYCH GANUL
Authors: Exp Oncology Editorial Board
Published: January 2019

Ukrainian oncology suffered a grievous loss. In March 29, 2015, aged 85, Valentyn Leonidovych Ganul died.
MODIFYING EFFECTS OF LACTOFERRIN IN VITRO ON MOLECULAR PHENOTYPE OF HUMAN BREAST CANCER CELLS
Authors: Chekhun V F , Zalutskii I V , Naleskina L A , Lukianova N Yu , Yalovenko T M , Borikun T , Sobchenko S O , Semak I V , Lukashevich V S
Published: January 2019

Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 breast cell lines of different origin — MCF-10 A, MCF-7, including doxorubicin/ cisplatin resistant ones, T47D, MDA-MB-231, and MDA-MB-468. Immunocytochemistry: e xpression of LF, Ki-67, adhesion molecules E- and N-cadherin, CD44, CD24 rating the invasive potential of cells. Results: Expression of LF in human BC cell lines varies. It is associated with the heterogeneity of molecular profiles of cell lines in terms of adhesion. A link has been established between the level of LF expression in the resistant cell line MCF-7/CP and MCF-7/Dox, features of their molecular profile and invasive properties. Exogenous LF was shown to be capable of modifying the molecular profile and invasive properties of all the studied cell lines including resistant ones (MCF-7/CP and MCF-7/Dox). Conclusions: The sensitivity of cytostatic-resistant cell lines (MCF-7/CP and MCF-7/Dox) tends to increase under the influence of exogenous LF. It is likely that this effect is due to LF-mediated inhibition of the expression of proteins associated with drug resistance.
TRP GENES FAMILY EXPRESSION IN COLORECTAL CANCER
Authors: Sozucan Y , Kalender M E , Sari I , Suner A , Oztuzcu S , Arman K , Yumrutas O , Bozgeyik I , Cengiz B , Igci Y Z , Balakan O , Camci C
Published: January 2019

Colorectal cancer (CRC) is the most common cancer of the gastrointestinal tract. Different factors are responsible for the development of CRC. Transient Receptor Potential (TRP) which is an important component of calcium channel is associated with several pathological conditions like cancer, neurodegenerative and cardiovascular diseases. Thirty members of the family of TRP ion channel in mammals have been determined till now. The aim of this study is to investigate TRPM, TRPV and TRPC gene expression levels in tumor tissues of CRC patients and to analyze the relationship of expression in tumor tissue of CRC with other known prognostic factors. Material and Methods: In this study, 93 CRC patients were included. The level of TRP gene expression in paraffin blocks of normal and cancerous colorectal tissue samples were studied at the level of mRNA with Real-time PCR. Results: The mRNA expression level of TRPV3, TRPV4, TRPV5, TRPM4 and TRPC6 genes in 37 female and 56 male patients diagnosed with CRC was revealed lower in tumor tissue as compared to normal tissue (p < 0.05). No statistically significant differences of mRNA expression levels of other TRP genes were found. Conclusions: TRP gene family like TRPV3, TRPV4, TRPV5, TRPM4 and TRPC6 may be thought as potential genes contributing to tumorigenesis as their expression decreases in CRC as compared to normal tissues.
GREEN TEA, RED WINE AND LEMON EXTRACTS REDUCE EXPERIMENTAL TUMOR GROWTH AND CANCER DRUG TOXICITY
Authors: Zaletok S , Gulua L , Wicker L , Shlyakhovenko V O , Gogo S , Orlovsky O , Karnaushenko O , Verbinenko A , Milinevska V , Samoylenko O , Todor I , Turmanidze T
Published: January 2019

Aim: To evaluate antitumor effect of plant polyphenol extracts from green tea, red wine lees and/or lemon peel alone and in combination with antitumor drugs on the growth of different transplanted tumors in experimental animals. Materials and Methods: Green tea extract (GTE) was prepared from green tea infusion. GTE-based composites of red wine (GTRW), lemon peel (GTRWL) and/ or NanoGTE as well as corresponding nanocomposites were prepared. The total polyphenolics of the different GTE-based extracts ranged from 18.0% to 21.3%. The effects of GTE-based extracts were studied in sarcoma 180, Ehrlich carcinoma, B16 melanoma, Ca755 mammary carcinoma, P388 leukemia, L1210 leukemia, and Guerin carcinoma (original, cisplatin-resistant and doxorubicinresistant variants). The extracts were administered as 0.1% solution in drinking water (0.6–1.0 mg by total polyphenolics per mouse per day and 4.0–6.3 mg per rat per day). Results: Tumor growth inhibition (TGI) in mice treated with NanoGTE, cisplatin or cisplatin + NanoGTE was 27%, 55% and 78%, respectively, in Sarcoma 180%, 21%, 45% and 59%, respectively, in Ehrlich carcinoma; and 8%, 13% and 38%, respectively in B16 melanoma. Composites of NanoGTE, red wine, and lemon peel (NanoGTRWL) enhanced the antitumor effects of cyclophosphamide in mice with Ca755 mammary carcinoma. The treatment with combination of NanoGTE and inhibitors of polyamines (PA) synthesis (DFMO + MGBG) resulted in significant TGI of P388 leukemia (up to 71%) and L1210 leukemia. In rats transplanted with Guerin carcinoma (parental strain), treatment with GTRW or GTE alone resulted in 25– 28% TGI vs. 55–68% TGI in cisplatin-treated animals. The inhibition observed in the case of combination of GTE or GTRW with cisplatin was additive giving 81–88% TGI. Similar effects were observed when combinations of the cytostatics with GTE (or N anoGTE) were tested against cisplatin- or doxorubicin-resistant Guerin carcinoma. Moreover, the plant extracts lowered side toxicity of the drugs. Treatment with GTE, NanoGTE, and NanoGTRW decreased the levels of malondialdehyde in heart, kidney and liver tissue of experimental animals, as well as the levels of urea and creatinine in blood serum, increased erythrocyte and platelet counts, hemoglobin content, and decreased leucocyte counts. Conclusion: The obtained data indicate the prospects for further deve lopment of GTE and corresponding nanocomposites as auxiliary agents in cancer chemotherapy.
THE STUDY OF MISMATCH REPAIR IN ENDOMETRIAL CANCER PATIENTS WITH A FAMILY HISTORY OF CANCER
Authors: Buchynska L G , Brieieva O , Nekrasov K N , Nespryadko S V
Published: January 2019

Aim: To assess the expression of mismatch repair (MMR) proteins MSH2 and MLH1 and carry out microsatellite analysis in patients with endometrial cancer (EC) with regard to the family history of cancer. Materials and Methods: Morphological and immunohistochemical study was performed on tumor tissue samples of 49 EC patients. Microsatellite instability was determined using PCR with primers which flank microsatellite region BAT-26. Results: A tendency to a decreased expression of both MSH2 and MLH1 markers in a group of EC patients with a family history of cancer as compared with a group without aggregation of cancer in family history was observed (labeling index — LI — was 36.1 ± 8.1% and LI 20.7 ± 9.1% versus LI 48.0 ± 5.8% and 33.8 ± 5.8%, respectively). It was determined that the number of EC patients with tumors deficient by expression of MMR markers was reliably higher in a group of patients with a family history of cancer than in a group of patients without aggregation of cancer in fami ly history (р < 0.05). It was shown that in a group of EC patients with a family history of cancer, MMR-proficient tumors were detected in 38.5% of cases. Microsatellite instability was determined in 10.7% of EC patients including one patient with aggregation of Lynch-associated tumors in family history. Conclusion: Family history of cancer of EC patients is associated with malfunctioning of the MMR system as well as may be related to alternative molecular mechanisms.
IMPACT OF KRAS VARIANT rs61764370 ON BREAST CANCER MORBIDITY
Authors: Ustinova M , Daneberga Z , Berzina D , Nakazawa-Miklasevica M , Maksimenko J , Gardovskis J , Miklasevics E
Published: January 2019

Low-penetrance gene variants and their combinations are topical study objects in breast cancer pathogenesis. Single nucleotide polymorphism rs61764370, localized in 3 UTR of KRAS gene, plays an important role in the development and progression of several cancers. The aim of our study was to determine the KRAS variant impact on breast cancer morbidity. Patients and Methods: 2214 patients diagnosed with breast cancer and 861 healthy controls were screened for KRAS variant by RFLP method. Available clinical data were collected and processed using statistical analysis methods. Results of present study suggest the KRAS variant impact on breast cancer development risk in premenopausal women, but it has no effect on breast cancer prognosis. We did not observe any KRAS variant effect on breast cancer patient 10-year disease-specific survival rates.
Photocytotoxic effect of c60 fullerene against l1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation
Authors: Franskevych D V , Grynyuk I I , Prylutska S V , Pasichnyk G V , Petukhov D M , Drobot L B , Matyshevska O P , Ritter U
Published: January 2019

Aim: To estimate the combined action of C60 fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lamp (λ = 410–700 nm, 2.45 J/cm2) was used for C60 fullerene photoexcitation. Nitrite level and NO-synthase activity were measured by Griess reaction and by conversion of L-arginine to L-citrulline, respectively. p38 MAPK activity was assessed by Western blot analysis. Cell cycle distribution was determined by flow cytometry. Results: It was shown that light irradiation of C60 fullerene-treated L1210 cells was accompanied by 55% decrease of their viability at 48 h of culture. Nitrite level measured as an index of reactive NO generation was increased at the early period after C60 fullerene photoexcitation due to activation of both constitutive and inducible NO-synthase isoforms. The simultaneous activation of p38 MAPK was detected. Accumulation of L1210 cells in sub-G1 phase of cell cycle was observed after C60 fullerene photoexcitation. Conclusion: Photoexcited C60 fullerene exerts cytotoxic effect, at least in part, through triggering production of reactive NO species and activation of p38 kinase apoptotic pathways in L1210 leukemic cells.
ENTEROSORPTION COMBINED WITH GRANULOCYTE COLONY STIMULATING FACTOR DECREASES MELPHALAN GONADAL TOXICITY
Authors: Shevchuk O O , Bodnar Ya Ya , Bardakhivska K I , Datsko T V , Volska A S , Posokhova K A , Chekhun V F , Nikolaev V G
Published: January 2019

Today due to improvements in cancer treatment there is an increasing number of long-term cancer survivors, many of whom suffer from infertility caused by malignancy itself and chemo- or radiotherapy. Also, anticancer therapy may cause myelosuppression. Presently granulocyte colony stimulating factor (G-CSF) is used for prevention and treatment of myelosuppression. Another treatment option used to decrease intoxication and ameliorate side effects of cancer therapy is sorption technology. The aim of our investigation was to study the efficiency of combined use of enterosorption and G-CSF to decrease gonadal toxicity of chemotherapy. Materials and Methods: Melphalan (L-PAM) injected i.v. at a single dose of 4 mg/kg to white inbred rats was used as gonadotoxic and myelosuppressing agent. Carbon enterosorbent C2 was administered by intragastric route as a suspension in saline at a dose of 5 ml per 1 kg of rats’ body weight (or 900 mg/kg of the dry mass of enterosorbent) daily for 3 days before and for 7 days after L-PAM injection. G-CSF was injected once a day for 4 days starting from the next day after L-PAM administration at a dose of 50 µg/kg. Histological preparations of testicular tissues were examined by light microscopy. Results: Our findings have shown that melphalan caused marked damage of testicular tissues and seminiferous, especially spermatogenic epithelium. The most expressed protection of the histological structure of testes was observed when enterosorbent and G-CSF were used in combination. Conclusion: Gonadal toxicity of chemotherapy could be efficiently decreased by the combined use of enterosorption and G-CSF.
LACTOFERRIN EXPRESSION IN BREAST CANCER IN RELATION TO BIOLOGIC PROPERTIES OF TUMORS AND CLINICAL FEATURES OF DISEASE
Authors: Naleskina L A , Lukianova N Yu , Sobchenko S O , Storchai D M , Chekhun V F
Published: January 2019

Aim: To determine the patterns of lactoferrin (LF) expression in breast cancer (BC) in relation to biologic properties of the neoplasms and clinical features of the disease course. Materials and Methods: Clinical specimens of 266 BC patients (115 patients with BC of stages I–II — retrospective study, and 151 BC patients — prospective study) were analyzed. Morphological, immunohistochemical and statistical methods were used. Results: The number of LF-positive tumors in retrospective and prospective groups was similar (52.1 and 52.8%, respectively). Among common clinical criteria for prognosis of the disease outcome in BC patients (patient’s age; stage of the disease; histological type, differentiation grade, receptor status; presence of metastases), a strong correlation was found only between expression indexes of LF and estrogen receptors (ER). In ER-positive tumors expression of LF was significantly higher than in ER-negative tumors (35 vs 18%). 5-Year survival rate of BC patients was higher in LF-positive group (70 vs 52% in LF-negative group). The presence of regional metastasis tended to correlate with an increased number of LF-positive tumors. In the patients with invasive ductal carcinoma, expression level of LF moderately correlated with occurrence of luminal A subtype (r = 0.43), while in the patients with invasive lobular carcinoma this index strongly correlated with occurrence of luminal B subtype (r = 0.71). LF expression correlated positively with low and moderate differentiation grade of luminal B or basal tumors, and negatively with luminal B or basal tumors of high differentiation grade (r = −0.57 and −0.63, respectively). Conclusion: It has been shown that LF expression in breast tumors correlated with life expectancy of BC patients and important physiologic and clinical features of the disease, while the character of such relation strongly depended on molecular phenotype of tumor, i.e. luminal A, luminal B or basal.
CHRONIC MYELOID LEUKEMIA IN PATIENT WITH THE KLINEFELTER SYNDROME
Authors: Andreieva S V , Korets K V , Kyselova O A , Ruzhinska O E , Serbin I M
Published: January 2019

Aim: Genetic inborn along with acquired diseases arise due to the lesions in genome of multipotent hematopoietic stem cells. The aim was to study an influence of constitutional anomaly, Klinefelter syndrome, and additional structural rearrangements on the BCR-ABL tyrosine kinase inhibitor targeted therapy efficacy. Material and Methods: We describe a 32-year-old male patient with chronic myeloid leukemia (CML) who was detected to have sex chromosomal abnormality during evaluation for Philadelphia chromosome. Results: At diagnosis of CML, two clones were detected by standard cytogenetic investigation of bone marrow cells: 1) clone with translocation t(9;22)(q34;q11), with two sex X chromosomes and absence sex chromosome Y; 2) clone with t(9;22) and unbalanced t(Y;20)(q11;q13). Analysis of blast transformed lymphocytes from peripheral blood showed karyotype 47,XXY. Monitoring of targeted therapy with second generation inhibitor of BCR-ABL tyrosine kinase indicated a cytogenetic remission and absence of BCR-ABL1 fusion signals after 11 months. Conclusions: Absence of translocation t(9;22)(q34;q11) in blast transformed T-lymphocytes at diagnosis of CML evidences that this translocation may appear not only at the level of multipotent haemopoietic cell progenitors but also may have oligo lineage myeloid origin. Presence of additional structural chromosomal abnormality in the clone with t(9;22)(q34;q11) does not affect the efficacy of therapy with the use of second generation BCR-ABL tyrosine kinase inhibitor.
SEVERAL ASPECTS OF DESCRIPTIVE EPIDEMIOLOGY OF HEMATOLOGICAL MALIGNANCIES IN ADULT POPULATION OF UKRAINE, BELARUS AND RUSSIAN FEDERATION AFTER CHORNOBYL ACCIDENT
Authors: Guslitser L N , Zavelevich M , Koval S , Gluzman D F
Published: January 2019

Chornobyl impact on the health of adult population in Ukraine, Belarus and Russian Federation was a subject of several studies. However, the studies of the effects of Chornobyl on leukemia in adult populations in post-Soviet countries are scarce and the results are contradictory up to present. The results of the epidemiological studies of the oncohematological consequences of Chornobyl accident are briefly reviewed with particular focus on pre-Chornobyl and post-Chornobyl trends in leukemia incidence in Ukraine, Belarus and Russian Federation as well as in small territories of these countries with various levels of radionuclide contamination. This article is a part of a Special Issue entitled “The Chornobyl Nuclear Accident: Thirty Years After”.
CHORNOBYL CATASTROPHE: CYTOGENETIC EFFECTS OF LOW DOSE IONIZING RADIATION AND THEIR MODIFICATION
Authors: Domina E A
Published: January 2019

Among the long-term effects of the Chornobyl disaster the greatest concern of international medical and scientific community is given to the established fact of excess of the spontaneous level of cancer incidence in the exposed population. According to modern concepts, the accumulation of chromosome aberrations, especially in radiosensitive cells, could be potentially oncogenic, and low doses of ionizing radiation could be promoters of the radiation-induced carcinogenesis. The results of our studies have shown that such substances as thymalin, inosine, ascorbic acid, caffeine could modify radiation-induced cytogenetic effects in peripheral blood lymphocytes of healthy individuals and exert protective or sensitizing action dependent on their concentrations, cell radiosensitivity, dose of irradiation, and relative biologic effectiveness of ionizing radiation. This article is part of a Special Issue entitled “The Chornobyl Nuclear Accident: Thirty Years After”.
CIRCULATING HEMATOPOIETIC PROGENITOR CELLS IN PATIENTS AFFECTED BY CHORNOBYL ACCIDENT
Authors: Bilko N M , Dyagil I S , Russu I S , Bilko D I
Published: January 2019

High radiation sensitivity of stem cells and their ability to accumulate sublethal radiation damage provides the basis for investigation of hematopoietic progenitors using in vivo culture methodology. Unique samples of peripheral blood and bone marrow were derived from the patients affected by Chornobyl accident during liquidation campaign. Aim: To investigate functional activity of circulating hematopoietic progenitor cells from peripheral blood and bone marrow of cleanup workers in early and remote periods after the accident at Chornobyl nuclear power plant (CNPP). Materials and Methods: The assessment of the functional activity of circulating hematopoietic progenitor cells was performed in samples of peripheral blood and bone marrow of 46 cleanup workers, who were treated in the National Scientific Center for Radiation Medicine of the Academy of Medical Sciences of Ukraine alongside with 35 non radiated patients, who served as a control. Work was performed by culturing peripheral blood and bone marrow mononuclear cells in the original gel diffusion capsules, implanted into the peritoneal cavity of CBA mice. Results: It was shown that hematopoietic progenitor cells could be identified in the peripheral blood of liquidators of CNPP accident. At the same time the number of functionally active progenitor cells of the bone marrow was significantly decreased and during the next 10 years after the accident, counts of circulating progenitor cells in the peripheral blood as well as functionally active hematopoietic cells in bone marrow returned to normal levels. Conclusion: It was shown that hematopoietic progenitor cells are detected not only in the bone marrow but also in the peripheral blood of liquidators as a consequence of radiation exposure associated with CNPP accident. This article is a part of a Special Issue entitled “The Chornobyl Nuclear Accident: Thirty Years After”.
POST-CHORNOBYL REMOTE RADIATION EFFECTS ON HUMAN SPERM AND SEMINAL PLASMA CHARACTERISTICS
Authors: Andreychenko S V , Klepko A V , Gorban L V , Motryna O A , Grubska L V , Trofimenko O V
Published: January 2019

Aim: The research was aimed on analysis of the remote consequences of Chornobyl accident on the reproductive function of men adult residing in Ukraine. Materials and Methods: 232 male volunteers with mean age of 34 years (range 20–47) from 5 different regions of Ukraine (Zhytomyr, Ivano-Frankivsk, Kyiv, Poltava, and Kyiv city) were enrolled in cross-sectional studies of long term radiation effects on seminal plasma and sperm. All manipulations, analysis and classification of ejaculates were done accordingly to WHO recommendations. The content of neutral α-glucosidase, fructose, citric acid in the seminal plasma was determined by spectrophotometry, L-carnitine — by high performance liquid chromatography, zinc — by atomic absorption spectroscopy. Results: In the men residing in the regions heavily contaminated with radioonuclides, the decreased sperm quality with concurrent asthenozoospermia, oligozoospermia, teratozoospermia, asthenoteratozoospermia, oligoasthenoteratozoospermia and oligoteratozoospermia was revealed. Moreover, the concomitant shifts in seminal plasma content of neutral α-glucosidase, fructose, citric acid, L-carnitine and zinc were detected. Conclusion: The study has revealed the ample sperm and semen abnormalities amongst the inhabitants of radiation polluted territories that should have to be a subject of careful research in forthcoming years. This article is a part of a Special Issue entitled “The Chornobyl Nuclear Accident: Thirty Years After”.
ASSOCIATION OF CD44+CD24−/low WITH MARKERS OF AGGRESSIVENESS AND PLASTICITY OF CELL LINES AND TUMORS OF PATIENTS WITH BREAST CANCER
Authors: Chekhun V F , Lukianova N Yu , Chekhun S V , Bezdieniezhnykh N O , Zadvorniy T V , Borikun T V , Polishchuk L Z , Klyusov O M
Published: January 2019

Aim: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. Materialts and Methods: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathioneS-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method. Expression of ER, PR, Her2/neu, CD24 and CD44 in the surgical material of BC patients were determined by the immunohistochemical method. The levels of the miRNA were determined using real-time polymerase chain reaction. Results: Cells of high-grade malignancy (HGM), MDA-MB-231 and MDA-MB-468 are characterized by high expression of stem cell markers compared to the cells of low-grade malignancy (LGM), T47D and MCF-7: CD44 levels in T47D and MCF-7 cells were in range of 72–79 points, which is significantly lower than in HGM cells (p < 0.05). Also, HGM cells with the properties of CSC were characterized by high expression of antiapoptotic proteins, the transcription factor Slug, and low levels of proapoptotic protein Bax (p < 0.05) compared to LGM cells. In cells with CSC characteristics an increased expression of transferrin and its receptor, ferritin, fentorin and hepcidin was revealed indicating activation of the endogenous iron metabolism. The characteristic feature of HGM cells with CSC phenotype were the increased levels of oncogenic miR-221, -155 and -10b by 60%, 92% and 78%, respectively, and decreased levels of oncosuppressive miR-29b, -34a and -200b by 8.4 ± 0.3, 4.6 ± 0.2, and 3.4 ± 0.6 times compared to MCF-7 line cells. It has been established that the development of resistance to cytostatics is accompanied by increased aggressiveness of tumor cells, loss of expression of hormonal receptors and acquiring of stem phenotype. In particular, increased expression of P-gp was observed in BC cells during the development of resistance to doxorubicin, of GST during the development of resistance to cisplatin along with increased CD44 expression (p < 0.05). We have revealed the relation between the presence of cells with the CSC phenotype (CD44+CD24-/low) and clinical and pathological characteristics of BC patients, their survival and BC sensitivity to neoadjuvant therapy (p > 0.05). Conclusions: The dependence between the expression of CSC markers and the degree of malignancy of tumor cells, development of resistance to cytostatics in vitro was established as well as the predictive value of the detection of the CSC for the individual prognosis of the BC course and sensitivity of the tumors to the treatment.
TGF-β1 EXPRESSION BY GLIOMA C6 CELLS IN VITRO
Authors: Liubich L D , Kovalevska L M , Lisyany M I , Semenova V M , Malysheva T A , Stayno L P , Vaslovych V V
Published: January 2019

The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS.
MADRASAH DAN PERUBAHAN SOSIAL
Authors: Ramadhani Rizki
Published: January 2019

AbstrakMadrasah merupakan lembaga pendidikan Islam yang mengintegrasikan ilmu-ilmu agama dengan ilmu umum. Ia berakar dari sistem pembelajaran pondok pesantren yang kemudian dipadukan dengan sistem pembelajaran klasikal ala Barat. Madrasah dengan eksistensinya selama satu Abad terakhir ini, telah memberikan sumbangan yang besar bagi perubahan sosial (progress) yang ada pada masyarakat Indonesia. Dengan adanya madrasah ini pula, pendidikan di Indonesia semakin berwarna, sehingga masyarakat memiliki banyak alternatif pendidikan untuk anak cucunya.Kata Kunci : Madrasah, Perubahan SosialAbstractMadrasah is Islamic education institute which integrate religion science and general science. It comes from system of pondok pesantren then combined with system of classical learning ala western. Madrasah with its existance along the last a hundred years, gives  a big contributions for social change (progress) to Indonesian. By the existante of madrasah, education in Indonesia become variative, so the social has many alternative education for their decendants.Keyword : Madrasah,  Social Change