bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolving Roles of Muscle-Resident Fibro-Adipogenic Progenitors in Health, Regeneration, Neuromuscular Disorders, and Aging
Authors: Theret Marine , Rossi Fabio M. V. , Contreras Osvaldo
Published: April 2021

Normal skeletal muscle functions are affected following trauma, chronic diseases, inherited neuromuscular disorders, aging, and cachexia, hampering the daily activities and quality of life of the affected patients. The maladaptive accumulation of fibrous intramuscular connective tissue and fat are hallmarks of multiple pathologies where chronic damage and inflammation are not resolved, leading to progressive muscle replacement and tissue degeneration. Muscle-resident fibro-adipogenic progenitors are adaptable stromal cells with multilineage potential. They are required for muscle homeostasis, neuromuscular integrity, and tissue regeneration. Fibro-adipogenic progenitors actively regulate and shape the extracellular matrix and exert immunomodulatory functions via cross-talk with multiple other residents and non-resident muscle cells. Remarkably, cumulative evidence shows that a significant proportion of activated fibroblasts, adipocytes, and bone-cartilage cells, found after muscle trauma and disease, descend from these enigmatic interstitial progenitors. Despite the profound impact of muscle disease on human health, the fibrous, fatty, and ectopic bone tissues’ origins are poorly understood. Here, we review the current knowledge of fibro-adipogenic progenitor function on muscle homeostatic integrity, regeneration, repair, and aging. We also discuss how scar-forming pathologies and disorders lead to dysregulations in their behavior and plasticity and how these stromal cells can control the onset and severity of muscle loss in disease. We finally explore the rationale of improving muscle regeneration by understanding and modulating fibro-adipogenic progenitors’ fate and behavior.
Generation of Marker-Free Transgenic Rice Resistant to Rice Blast Disease Using Ac/Ds Transposon-Mediated Transgene Reintegration System
Authors: Li Xin , Pan Longyu , Bi Dongling , Tian Xudan , Li Lihua , Xu Zhaomeng , Wang Lanlan , Zou Xiaowei , Gao Xiaoqing , Yang Haihe , Qu Haiyan , Zhao Xiangqian , Yuan Zhengjie , He Haiyan , Qu Shaohong
Published: April 2021

Rice blast is one of the most serious diseases of rice and a major threat to rice production. Breeding disease-resistant rice is one of the most economical, safe, and effective measures for the control of rice blast. As a complement to traditional crop breeding, the transgenic method can avoid the time-consuming process of crosses and multi-generation selection. In this study, maize (Zea mays) Activator (Ac)/Dissociation (Ds) transposon vectors carrying green fluorescent protein (GFP) and red fluorescent protein (mCherry) genetic markers were used for generating marker-free transgenic rice. Double fluorescent protein-aided counterselection against the presence of T-DNA was performed together with polymerase chain reaction (PCR)-based positive selection for the gene of interest (GOI) to screen marker-free progeny. We cloned an RNAi expression cassette of the rice Pi21 gene that negatively regulates resistance to rice blast as a GOI into the Ds element in the Ac/Ds vector and obtained marker-free T1 rice plants from 13 independent transgenic lines. Marker-free and Ds/GOI-homozygous rice lines were verified by PCR and Southern hybridization analysis to be completely free of transgenic markers and T-DNA sequences. qRT-PCR analysis and rice blast disease inoculation confirmed that the marker-free transgenic rice lines exhibited decreased Pi21 expression levels and increased resistance to rice blast. TAIL-PCR results showed that the Ds (Pi21-RNAi) transgenes in two rice lines were reintegrated in intergenic regions in the rice genome. The Ac/Ds vector with dual fluorescent protein markers offers more reliable screening of marker-free transgenic progeny and can be utilized in the transgenic breeding of rice disease resistance and other agronomic traits.
Correlation Between Physiological and Performance-Based Metrics to Estimate Pilots' Cognitive Workload
Authors: Hebbar P. Archana , Bhattacharya Kausik , Prabhakar Gowdham , Pashilkar Abhay A. , Biswas Pradipta
Published: April 2021

This paper discusses the utilization of pilots' physiological indications such as electroencephalographic (EEG) signals, ocular parameters, and pilot performance-based quantitative metrics to estimate cognitive workload. The study aims to derive a non-invasive technique to estimate pilot's cognitive workload and study their correlation with standard physiological parameters. Initially, we conducted a set of user trials using well-established psychometric tests for evaluating the effectiveness of pupil and gaze-based ocular metrics for estimating cognitive workload at different levels of task difficulty and lighting conditions. Later, we conducted user trials with the NALSim flight simulator using a business class Learjet aircraft model. We analyzed participants' ocular parameters, power levels of different EEG frequency bands, and flight parameters for estimating variations in cognitive workload. Results indicate that introduction of secondary task increases pilot's cognitive workload significantly. The beta frequency band of EEG, nearest neighborhood index specifying distribution of gaze fixation, L1 Norm of power spectral density of pupil diameter, and the duty cycle metric indicated variations in cognitive workload.
The Benefit of Gratitude: Trait Gratitude Is Associated With Effective Economic Decision-Making in the Ultimatum Game
Authors: Park Gewnhi , vanOyen-Witvliet Charlotte , Barraza Jorge A. , Marsh Benjamin U.
Published: April 2021

The current research investigated the role of gratitude in economic decisions about offers that vary in fairness yet benefit both parties if accepted. Participants completed a trait/dispositional gratitude measure and then were randomly assigned to recall either an event that made them feel grateful (i.e., induced gratitude condition) or the events of a typical day (i.e., neutral condition). After the gratitude induction task, participants played the ultimatum game (UG), deciding whether to accept or reject fair offers (i.e., proposer: responder ratio $5:5) and unfair offers (i.e., proposer: responder ratios of $9:1, $8:2, or $7:3) from different proposers. Results showed that trait gratitude was positively correlated with respondents’ acceptance of unfair offers. However, experimentally induced momentary gratitude did not influence acceptance of unfair offers. The trait or disposition to be grateful involves the enduring capacity across different types of situations and benefactors to see the good that is present, even when that benefit is small. Accordingly, dispositional gratitude – but not momentarily induced gratitude – was associated with a greater propensity to accept even the small benefits within unfair offers which otherwise pose barriers to making the effective economic decision of accepting offers regardless of their relative size.
Short-Term Memory for Serial Order Moderates Aspects of Language Acquisition in Children With Developmental Language Disorder: Findings From the HelSLI Study
Authors: Lahti-Nuuttila Pekka , Service Elisabet , Smolander Sini , Kunnari Sari , Arkkila Eva , Laasonen Marja
Published: April 2021

Previous studies of verbal short-term memory (STM) indicate that STM for serial order may be linked to language development and developmental language disorder (DLD). To clarify whether a domain-general mechanism is impaired in DLD, we studied the relations between age, non-verbal serial STM, and language competence (expressive language, receptive language, and language reasoning). We hypothesized that non-verbal serial STM differences between groups of children with DLD and typically developing (TD) children are linked to their language acquisition differences. Fifty-one children with DLD and sixty-six TD children participated as part of the HelSLI project in this cross-sectional study. The children were 4–6-year-old monolingual native Finnish speakers. They completed several tests of language and cognitive functioning, as well as new game-like tests of visual and auditory non-verbal serial STM. We used regression analyses to examine how serial STM moderates the effect of age on language. A non-verbal composite measure of serial visual and auditory STM moderated cross-sectional development of receptive language in the children with DLD. This moderation was not observed in the TD children. However, we found more rapid cross-sectional development of non-verbal serial STM in the TD children than in the children with DLD. The results suggest that children with DLD may be more likely to have compromised general serial STM processing and that superior non-verbal serial STM may be associated with better language acquisition in children with DLD.
Emoji as Affective Symbols: Affective Judgments of Emoji, Emoticons, and Human Faces Varying in Emotional Content
Authors: Fischer Brigitte , Herbert Cornelia
Published: April 2021

An important function of emoji as communicative symbols is to convey emotional content from sender to receiver in computer-mediated communication, e. g., WhatsApp. However, compared with real faces, pictures or words, many emoji are ambiguous because they do not symbolize a discrete emotion or feeling state. Thus, their meaning relies on the context of the message in which they are embedded. Previous studies investigated affective judgments of pictures, faces, and words suggesting that these stimuli show a typical distribution along the big two emotion dimensions of valence and arousal. Also, emoji and emoticons have been investigated recently for their affective significance. The present study extends previous research by investigating affective ratings of emoji, emoticons and human faces and by direct comparison between them. In total, 60 stimuli have been rated by 83 participants (eight males, age: 18–49 years), using the non-verbal Self-Assessment Manikin Scales for valence and arousal. The emotionality of the stimuli was measured on a 9-point Likert scale. The results show significant main effects of the factors “stimulus category” and “discrete emotion” including emotionality, valence and arousal. Also, the interaction between these two main factors was significant. Emoji elicited highest arousal, whereas stimuli related to happiness were rated highest in valence across stimulus categories. Angry emoji were rated highest in emotionality. Also, the discrete emotion was best recognized in emoji, followed by human face stimuli and lastly emoticons.
Academic Leadership in the Time of COVID-19—Experiences and Perspectives
Authors: Dumulescu Daniela , Muţiu Alexandra Ileana
Published: April 2021

The COVID-19 pandemic has been a sharp reminder that large scale, unpredictable events always bring about profound changes with significant consequences on many levels. In light of lockdown measures taken in many countries across the world to control the spread of the virus, academics were “forced” to adapt and move to online settings all teaching, mentoring, research, and support activities. Academic leaders in higher education had to make decisions and to act quickly how were they to manage large educational communities, addressing students', teachers', and staff's needs, as well as society's needs. Using an inductive approach, our study seeks to highlight the main challenges faced by university leaders and to understand their responses to those challenges. The current study aims to investigate perceptions and experiences of academic leaders in a University in Romania during the COVID-19 pandemic. Our foci were the processes underlying their leadership decisions and actions during the second part of the 2019–2020 academic year. Data was collected through semi-structured interviews with 11 university and faculty leaders in Babeş-Bolyai University, Romania. The findings from the thematic analysis revealed three main themes emerging from participants' responses: “the leader's personal attributes,” “unity through decentralization,” and “opportunities to reinvent the university.” Good practices to manage academic institutions in times of crises and changes are discussed, along with insights into strategies for supporting higher education development during crisis and post-crisis times resulting into recommendations for increasing management effectiveness.
Electrophysiological Correlates of Processing Warning Signs With Different Background Colors: An Event-Related Potentials Investigation
Authors: Yuan Jingpeng , Song Zhipeng , Hu Ying , Fu Huijian , Liu Xiao , Bian Jun
Published: April 2021

Warning signs, as a type of safety signs, are widely applied in our daily lives to informing people about potential hazards and prompting safe behavior. Although previous studies have paid attention to the color of warning signs, they are mostly based on surveys and behavioral experiments. The neural substrates underlying the perception of warning signs with different background colors remain not clearly characterized. Therefore, this research is intended to address this gap with event-related potentials (ERPs) technique. Warning signs with three different background colors (i.e., white, yellow, and blue) were used in the experiment. The results showed that the perceptual differences between different warning signs were present in the form of differential ERPs components (P1, N1, P2, N2, and P3) though subjects were not required to explicitly attend to the warning signs.
Looking Beyond Assumptions to Understand Relationship Dynamics in Bullying
Authors: Mishna Faye , Birze Arija , Greenblatt Andrea , Pepler Debra
Published: April 2021

To account for the complex relationships and processes that constitute the phenomenon of bullying, it is critical to understand how students and their parents and teachers conceptualize traditional and cyberbullying. Qualitative data were drawn from a mixed methods longitudinal study on cyberbullying. Semi-structured interviews were held with Canadian students in grades 4, 7, and 10 in a large urban school board, and their parents and teachers. To account for the complexity and interactions of different systems of relationships, the purpose of the current article is to examine how students and their matched parents and teachers understand traditional and cyberbullying. Central to participants' understanding of traditional and cyberbullying was whether they considered bullying to represent harmful relationship dynamics. Three main assumptions emerged as shaping participants' understanding of bullying and appeared to obscure the deep relationship processes in bullying: (a) assumptions of gender in bullying, (b) type of bullying—comparing traditional and cyberbullying, and (c) physical bullying as disconnected from relationship dynamics. It is essential that assessment, education, and prevention and intervention strategies in traditional and cyberbullying be informed by the inherent relationships in bullying and be implemented at multiple levels of relationships and broader social systems.
Cerebral Regional Homogeneity Alternation of Pregnant Women With Antenatal Depression During the Pandemic
Authors: Cheng Bochao , Meng Yajing , Zhou Yushan , Li Jinrong , Zeng Jianguang , Tan Xi , Zhang Kaiyou , Luo Ya , Zhang Yan
Published: April 2021

Purpose: The COVID-19 epidemic has been a threat to the health of people all over the world. Various precautions during COVID-19 in China have kept a large number of people in isolation, and this has inconvenienced and placed enormous stress on pregnant women. Pregnant women are more likely to suffer from antenatal depression (ANDP) with social isolation or low social support. This research aims to investigate the neurobiological mechanisms underlying ANDP, which impedes early detection and intervention in this disorder.

Methods: A total of 43 singleton pregnant women who experienced isolation were recruited, including 21 treatment-naïve ANDP patients and 22 healthy pregnant women (HPW). To explore the intrinsic cerebral activity alternations in ANDP using resting-state functional MRI (rsfMRI), we assessed the local regional homogeneity (ReHo) differences in two groups using the voxel-based whole-brain analysis. The correlation between the regional functional abnormalities and clinical variables in ANDP patients was also examined.

Results: Compared with HPW, ANDP patients showed decreased ReHo in the left dorsolateral prefrontal cortex, right insular and the cluster coving the right ventral temporal cortex (VTC), amygdala (AMG), and hippocampus (HIP). The Edinburgh Postnatal Depression Scale (EPDS) scores of ANDP patients negatively correlated with the ReHo in the right VTC, AMG, and HIP.

Conclusion: Elucidating the neurobiological features of ANDP patients during COVID-19 is crucial for evolving adequate methods for early diagnosis, precaution, and intervention in a future epidemic.
Assessment of Suicide Risks During the First Week Immediately After Discharge From Psychiatric Inpatient Facility
Authors: Madsen Trine , Egilsdottir Eybjørg , Damgaard Chanette , Erlangsen Annette , Nordentoft Merete
Published: April 2021

Background: The suicide rate in first week after psychiatric discharge is alarmingly high. Although a risk assessment prior to discharge is standard praxis, it can be difficult to take into consideration the obstacles that patient will meet once discharged. A follow-up-visit during the first week after discharge is an opportunity to reevaluate whether a person may be at risk of suicide.

Aim: To determine how many patients, of those who were assessed, were evaluated to be at elevated risk of suicide during the first week after psychiatric discharge and secondarily to identify predictors of this and predictors for receiving a follow-up visit during first week after discharge.

Methods: All patients discharged between March 1st 2018 to January 17th 2019 were offered a home visit including a systematic risk assessment. Socio-demographics and clinical variables were obtained from medical records and logistic regression analyses were used to identify predictors of a higher suicide risk assessment as well as receiving a follow-up visit.

Results: Information from 1905 discharges were included. Of these, 1,052 were seen in follow-up meetings. Risk assessments was conducted in a total of 567 discharge procedures, of which 28 (5%) had an elevated risk of suicide. A history of suicide attempt, suicide risk having been the reason for admission, a first diagnosis of a psychiatric disorder was associated with an elevated risk of suicide after discharge.

Conclusion: Follow-up visits could serve as an important tool to identify people whose suicidal risk were overlooked at discharge or exposed to severe stressors after discharge.
Case Report: Virtual Reality Neurofeedback Therapy as a Novel Modality for Sustained Analgesia in Centralized Pain Syndromes
Authors: Orakpo Nnamdi , Vieux Ulrick , Castro-Nuñez Cristian
Published: April 2021

Neurofeedback (NFB) Therapy is a form of biofeedback, using the electroencephalogram (EEG) that has been in use since the 1970s, serving as a non-pharmacological intervention for epilepsy and psychiatric conditions such as anxiety, depression, insomnia, PTSD, post-concussive syndrome, and now, centralized pain. Chronic pain can increase neuronal activity and eventually causes poor modulation of pain messages. With the emergence of Virtual Reality (VR) in acute pain management, and the contraindications of opioids in chronic pain, applying novel biotechnologies seems like the next frontier in multimodal pain management. In this study, the VR and NFB technologies were fused together (VR-NFB) and used as a novel treatment modality for a 55-year-old woman who suffered from chronic pain secondary to spondylolisthesis with cervical, thoracic, and lumbar disc herniations after a motor vehicle accident with comorbid depression, anxiety, sleep deprivation, and difficulty with activities of daily living, and inability to participate in physical therapy. Our case reports on the sustained analgesia achieved for 1 year after a trial of VR-NFB, and the usefulness of neuromodulation in centralized pain syndromes.
Barriers Influencing Vaccine Development Timelines, Identification, Causal Analysis, and Prioritization of Key Barriers by KOLs in General and Covid-19 Vaccine R&D
Authors: Janse Marga , Brouwers Thomas , Claassen Eric , Hermans Peter , van de Burgwal Linda
Published: April 2021

A frequently mentioned factor holding back the introduction of new vaccines on the market are their prohibitively long development timelines. These hamper their potential societal benefit and impairs the ability to quickly respond to emerging new pathogens. This is especially worrisome since new pathogens are emerging at all-time high rates of over one per year, and many age-old pathogens are still not vaccine preventable.Through interviews with 20 key-opinion-leaders (KOLs), this study identified innovation barriers that increase vaccine development timelines. These innovation barriers were visualized, and their underlying causes revealed by means of qualitative root cause analysis. Based on a survey the innovation barriers were quantitatively ranked based on their relative impact on both regular, and Covid-19 vaccine development timelines. KOLs identified 20 key innovation barriers, and mapping these barriers onto the Vaccine Innovation Cycle model revealed that all phases of vaccine development were affected. Affected by most barriers is the area between the preclinical studies and the market entry. Difficult hand-off between academia and industry, lack of funding, and lack of knowledge of pathogen targets were often mentioned as causes. Quantitative survey responses from 93 KOLs showed that general vaccine development and Covid-19 vaccine development are impacted by distinct sets of innovation barriers. For the general vaccine development three barriers were perceived of the highest impact; limited ROI for vaccines addressing disease with limited market size, limited ROI for vaccines compared to non-vaccine projects, and academia not being able to progress beyond proof of principle. Of highest impact on Covid-19 vaccine development, are lack of knowledge concerning pathogen target, high risk of upscaling unlicensed vaccines, and proof of principle not meeting late-stage requirements. In conclusion, the current study demonstrates that barriers hampering timelines in vaccine development are present across the Vaccine Innovation Cycle. Prioritizing the impact of barriers in general, and in Covid-19 vaccine development, shows clear differences that can be used to inform policies to speed up development in both war and peace time.
Comparison of Dexterous Task Performance in Virtual Reality and Real-World Environments
Authors: Joyner Janell S. , Vaughn-Cooke Monifa , Benz Heather L.
Published: April 2021

Virtual reality is being used to aid in prototyping of advanced limb prostheses with anthropomorphic behavior and user training. A virtual version of a prosthesis and testing environment can be programmed to mimic the appearance and interactions of its real-world counterpart, but little is understood about how task selection and object design impact user performance in virtual reality and how it translates to real-world performance. To bridge this knowledge gap, we performed a study in which able-bodied individuals manipulated a virtual prosthesis and later a real-world version to complete eight activities of daily living. We examined subjects' ability to complete the activities, how long it took to complete the tasks, and number of attempts to complete each task in the two environments. A notable result is that subjects were unable to complete tasks in virtual reality that involved manipulating small objects and objects flush with the table, but were able to complete those tasks in the real world. The results of this study suggest that standardization of virtual task environment design may lead to more accurate simulation of real-world performance.
Factors Influencing the Persistence of Salmonella Infantis in Broiler Litter During Composting and Stabilization Processes and Following Soil Incorporation
Authors: Avidov Ran , Varma Vempalli Sudharsan , Saadi Ibrahim , Hanan Aviva , Lublin Avishai , Saldinger Shlomo Sela , Chen Yona , Laor Yael
Published: April 2021

Broiler litter (BL), a by-product of broiler meat production, is frequently contaminated with Salmonella and other zoonotic pathogens. To ensure the safety of crop production chains and limit pathogen spread in the environment, a pre-treatment is desired before further agricultural utilization. The objective of this study was to characterize the effect of physico-chemical properties on Salmonella persistence in BL during composting and stabilization and following soil incorporation, toward optimization of the inactivation process. Thirty-six combinations of temperature (30, 40, 50, and 60°C), water content (40, 55, and 70%; w/w), and initial pH (6, 7, and 8.5) were employed in static lab vessels to study the persistence of Salmonella enterica serovar Infantis (S. Infantis; a multidrug-resistant strain) during incubation of artificially-inoculated BL. The effect of aeration was investigated in a composting simulator, with controlled heating and flow conditions. Temperature was found to be the main factor significantly influencing Salmonella decay rates, while water content and initial pH had a secondary level of influence with significant effects mainly at 30 and 40°C. Controlled simulations showed faster decay of Salmonella under anaerobic conditions at mesophilic temperatures (<45°C) and no effect of NH3 emissions. Re-wetting the BL at mesophilic temperatures resulted in Salmonella burst, and led to a higher tolerance of the pathogen at increased temperatures. Based on the decay rates measured under all temperature, water content, and pH conditions, it was estimated that the time required to achieve a 7 log10 reduction in Salmonella concentration, ranges between 13.7–27.2, 6.5–15.6, 1.2–4.7, and 1.3–1.5 days for 30, 40, 50, and 60°C, respectively. Inactivation of BL indigenous microbial population by autoclaving or addition of antibiotics to which the S. Infantis is resistant, resulted in augmentation of Salmonella multiplication. This suggests the presence of microbial antagonists in the BL, which inhibit the growth of the pathogen. Finally, Salmonella persisted over 90 days at 30°C in a Vertisol soil amended with inoculated BL, presumably due to reduced antagonistic activity compared to the BL alone. These findings are valuable for risk assessments and the formulation of guidelines for safe utilization of BL in agriculture.
Valorization of Vegetable Fresh-Processing Residues as Functional Powdered Ingredients. A Review on the Potential Impact of Pretreatments and Drying Methods on Bioactive Compounds and Their Bioaccessibility
Authors: Ramírez-Pulido Bibiana , Bas-Bellver Claudia , Betoret Noelia , Barrera Cristina , Seguí Lucía
Published: April 2021

Food waste is a worldwide concern as it represents a constant threat to the environment and a serious operational problem for the food industry. The by-products of fruits and vegetables being a valuable source of bioactive compounds have the potential to be reused and reintroduced in the agri-food chain. This circular approach contributes to a sustainable production system. In this context, a collaborative project with the primary sector for the integral valorization of the waste generated in the fresh-processing vegetable lines of an agricultural cooperative is currently being developed, particularly focused on cabbage, carrot, celery, and leek. The objective of this project is to transform vegetable wastes into functional powdered ingredients and be able to use them in food formulations in order to improve the nutritional profile of foods, contributing to the development of sustainable healthy diets. Through an exhaustive bibliographic review, this research studies the influence of pretreatments, drying and in vitro digestion on the bioactive compounds of vegetable residues, with the aim of identifying the appropriate production parameters to achieve an adequate functional and physicochemical profile of the final powders.
3D Printing Improved Testicular Prostheses: Using Lattice Infill Structure to Modify Mechanical Properties
Authors: Skewes Jacob , Chen Michael Y. , Forrestal David , Rukin Nicholas J. , Woodruff Maria A.
Published: April 2021

Patients often opt for implantation of testicular prostheses following orchidectomy for cancer or torsion. Recipients of testicular prostheses report issues regarding firmness, shape, size, and position, aspects of which relate to current limitations of silicone materials used and manufacturing methods for soft prostheses. We aim to create a 3D printable testicular prosthesis which mimics the natural shape and stiffness of a human testicle using a lattice infill structure. Porous testicular prostheses were engineered with relative densities from 0.1 to 0.9 using a repeating cubic unit cell lattice inside an anatomically accurate testicle 3D model. These models were printed using a multi-jetting process with an elastomeric material and compared with current market prostheses using shore hardness tests. Additionally, standard sized porous specimens were printed for compression testing to verify and match the stiffness to human testicle elastic modulus (E-modulus) values from literature. The resulting 3D printed testicular prosthesis of relative density between 0.3 and 0.4 successfully achieved a reduction of its bulk compressive E-modulus from 360 KPa to a human testicle at 28 Kpa. Additionally, this is the first study to quantitatively show that current commercial testicular prostheses are too firm compared to native tissue. 3D printing allows us to create metamaterials that match the properties of human tissue to create customisable patient specific prostheses. This method expands the use cases for existing biomaterials by tuning their properties and could be applied to other implants mimicking native tissues.
Clinical Randomized Comparison of Medetomidine and Xylazine for Isoflurane Balanced Anesthesia in Horses
Authors: Wiederkehr Alexandra , Barbarossa Andrea , Ringer Simone K. , Jörger Fabiola B. , Bryner Marco , Bettschart-Wolfensberger Regula
Published: April 2021

Introduction:To assess drug plasma levels, preanesthetic sedation, cardiopulmonary effects during anesthesia and recovery in horses anesthetized with isoflurane combined with medetomidine or xylazine.

Study design:Prospective blinded randomized clinical study.

Animals:Sixty horses undergoing elective surgery.

Methods:Thirty minutes after administration of antibiotics, flunixine meglumine or phenylbutazone and acepromazine horses received medetomidine 7 μg kg−1(group MED) or xylazine 1.1 mg kg−1(group XYL) slowly intravenously (IV) and sedation was assessed 3 min later. Anesthesia was induced with ketamine/diazepam and maintained with isoflurane in oxygen/air and medetomidine 3.5 μg kg−1h−1or xylazine 0.69 mg kg−1h−1. Ringer's acetate 10 mL kg−1h−1and dobutamine were administered to maintain normotension. All horses were mechanically ventilated to maintain end-tidal carbon dioxide pressures at 45 ± 5 mmHg (5.3–6.7 kPa). Heart rate (HR), invasive arterial blood pressures, inspired and expired gas compositions, pH, arterial blood gases, electrolytes, lactate and glucose were measured. For recovery all horses received intramuscular morphine 0.1 mg kg−1and medetomidine 2 μg kg−1or xylazine 0.3 mg kg−1IV. Recovery was timed and scored using three different scoring systems. Plasma samples to measure medetomidine and xylazine concentrations were collected at predetermined timepoints. Repeatedly measured parameters were analyzed using a two-way repeated-measures analysis of variance for differences between groups and over time;p< 0.05 was considered statistically significant.

Results:Mean arterial blood pressures (MAP) stayed within normal ranges but were higher (p= 0.011) in group XYL despite significant lower dobutamine doses (p= 0.0003). Other measured parameters were within clinically acceptable ranges. Plasma levels were at steady state during anesthesia (MED 2.194 ± 0.073; XYL 708 ± 18.791 ng mL−1). During recovery lateral recumbency (MED 42.7 ± 2.51; XYL 34.3 ± 2.63 min;p= 0.027) and time to standing (MED 62.0 ± 2.86; XYL 48.8 ± 3.01 min;p= 0.002) were significantly shorter in group XYL compared to group MED. Recovery scores did not differ significantly between groups.

Conclusion and Clinical Relevance:In horses anesthetized with isoflurane and medetomidine or xylazine, xylazine maintained higher MAP, reduced the dobutamine consumption and recovery time, whilst overall recovery quality was unaffected.
Genetic Diversity of Recent Infectious Bursal Disease Viruses Isolated From Vaccinated Poultry Flocks in Malaysia
Authors: Aliyu Hayatuddeen Bako , Hair-Bejo Mohd , Omar Abdul Rahman , Ideris Aini
Published: April 2021

Vaccination is an essential component in controlling infectious bursal disease (IBD), however, there is a lack of information on the genetic characteristics of a recent infectious bursal disease virus (IBDV) that was isolated from IBD vaccinated commercial flocks in Malaysia. The present study investigated 11 IBDV isolates that were isolated from commercial poultry farms. The isolates were detected using reverse transcription-polymerase chain reaction (RT-PCR) targeting the hypervariable region (HVR) of VP2. Based on the HVR sequences, five isolates (IBS536/2017, IBS624/2017, UPM766/2018, UPM1056/2018, and UPM1432/2019) were selected for whole-genome sequencing using the MiSeq platform. The nucleotide and amino acid (aa) sequences were compared with the previously characterized IBDV strains. Deduced aa sequences of VP2HVR revealed seven isolates with 94–99% aa identity to very virulent strains (genogroup 3), two isolates with 97–100% aa identity to variant strains (genogroup 2), and two strains with 100% identity to the vaccine strain (genogroup 1) of IBDV. The phylogenetic analysis also showed that the isolates formed clusters with the respective genogroups. The characteristic motifs 222T, 249K, 286I, and 318D are typical of the variant strain and were observed for UPM1219/2019 and UPM1432/2019. In comparison, very virulent residues such as 222A, 249Q, 286T, and 318G were found for the vvIBDV, except for the UPM1056/2018 strain with a A222T substitution. In addition, the isolate has aa substitutions such as D213N, G254D, S315T, S317R, and A321E that are not commonly found in previously reported vvIBDV strains. Unlike the other vvIBDVs characterized in this study, UPM766/2018 lacks the MLSL aa residues in VP5. The aa tripeptides 145/146/147 (TDN) of VP1 were conserved for the vvIBDV, while a different motif, NED, was observed for the Malaysian variant strain. The phylogenetic tree showed that the IBDV variant clustered with the American and Chinese variant viruses and are highly comparable to the novel Chinese variants, with 99.9% identity. Based on the sequences and phylogenetic analyses, this is the first identification of an IBDV variant being reported in Malaysia. Further research is required to determine the pathogenicity of the IBDV variant and the protective efficacy of the current IBD vaccines being used against the virus.
White and Red Sorghum as Primary Carbohydrate Sources in Extruded Diets of Felines
Authors: von Schaumburg Patrick , He Fei , Rodriguez-Zas Sandra L. , Southey Bruce R. , Parsons C. M. , de Godoy Maria R. C.
Published: April 2021

The research objectives were to evaluate the effect of dietary supplementation of white (WSH) and red (RSH) sorghum grains on gastrointestinal health of felines through the determination of apparent total tract macronutrient digestibility (ATTD), fecal characteristics, fermentative end-products, and microbiota, compared with a traditional corn-based diet. We hypothesize that inclusion of RSH and WSH, respectively, would be well-accepted by cats, and the RSH and WSH diets would be comparable to corn when added as the main carbohydrate source in extruded diets. Three diets containing 30% corn, 30% WSH, or 30% RSH were formulated to meet or exceed the AAFCO (2018) nutrient profiles for cats during growth. Nine male cats (0.8 ± 0.00 yr) were randomly assigned to one of the three dietary treatments using a triplicated 3 × 3 Latin square design. Experimental periods consisted of 14 d (10 d of diet adaption and 4 d of total and fresh fecal collections). The ATTD of dry matter (DM) did not differ amongst treatments, organic matter was greatest (P < 0.05) for both sorghum diets (86.4%) and lowest for the corn diet (84.2%), crude protein was comparable among diets ranging from 84.5 to 86.6%, acid hydrolyzed fat was high among diets varying between 91.4 and 92.8%, and total dietary fiber was greatest (P < 0.05) for the WSH diet (56.0%) with the corn diet being lowest (44.7%). Digestible energy was greatest (P < 0.05) for the WSH diet (4.66 kcal/g) and lowest for the corn diet (4.54 kcal/g), with the RSH diet being intermediate (4.64; P > 0.05). Fecal pH (6.3–6.5) and most fecal metabolites did not differ among diets except for phenol/indole concentrations that were significantly lower (P < 0.05) in cats fed the RSH diet (1.5 μmole/g DM) than for cats fed the corn diet (2.1 μmole/g DM). Bacteroidetes, Firmicutes, Fusobacteria, and Proteobacteria were the major phyla observed in the microbiota of feces of cats fed the three experimental diets, with no differences seen amongst all treatments. Data indicate that dietary supplementation of these varieties of WSH and RSH as carbohydrate sources were well-tolerated by the cat.
FOXF1 as an Immunohistochemical Marker of Hilar Cholangiocarcinoma or Metastatic Pancreatic Ductal Adenocarcinoma. Single Institution Experience
Authors: Hrudka Jan , Prouzová Zuzana , Mydlíková Katarína , Jedličková Kristína , Holešta Michal , Whitley Adam , Havlůj Lukáš
Published: April 2021

Cholangiocarcinoma (CCA) is a liver malignancy associated with a poor prognosis. Its main subtypes are peripheral/intrahepatic and hilar/extrahepatic CCA. Several molecular, morphological and clinical similarities between hilar/extrahepatic CCA and pancreatic ductal adenocarcinoma (PDAC) have been described. FOXF1 is a transcription factor which has been described to have prognostic significance in various tumors and it is involved in the development of bile ducts. The aim of this study is to determine occurrence of nuclear expression of FOXF1 in both subtypes of CCA and metastatic PDAC and assess its potential usefulness as a diagnostic marker. Secondary aims were to investigate the use of C-reactive protein (CRP) immunohistochemistry for diagnosing intrahepatic peripheral CCA and the significance of histological features in CCA subtypes. 32 archive specimens of CCA, combined hepatocellular carcinoma-CCA (HCC-CCA) and liver metastasis of PDAC were stained by FOXF1 and CRP immunohistochemistry and evaluated to determine histological pattern. The CCAs were classified radiologically into peripheral/intrahepatic and hilar subtype. Using Fisher exact test, we identified nuclear FOXF1 as a fairly specific (87%) but insensitive (65%) marker of hilar and extrahepatic CCA and metastatic PDAC (p = 0.005). CRP immunohistochemistry was characterized by a high sensitivity and specificity, of 79% and 88%, respectively (p = 0.001). We did not identify any histomorphological features associated with either types of CCA or metastatic PDAC. As a conclusion of novel finding, FOXF1 immunohistochemistry may be regarded as a specific but insensitive marker of hilar/extrahepatic CCA and metastatic PDAC and it may help distinguish them from peripheral CCA.
A Multinomial DGA Classifier for Incipient Fault Detection in Oil-Impregnated Power Transformers
Authors: Odongo George , Musabe Richard , Hanyurwimfura Damien
Published: April 2021

This study investigates the use of machine-learning approaches to interpret Dissolved Gas Analysis (DGA) data to find incipient faults early in oil-impregnated transformers. Transformers are critical pieces of equipment in transmitting and distributing electrical energy. The failure of a single unit disturbs a huge number of consumers and suppresses economic activities in the vicinity. Because of this, it is important that power utility companies accord high priority to condition monitoring of critical assets. The analysis of dissolved gases is a technique popularly used for monitoring the condition of transformers dipped in oil. The interpretation of DGA data is however inconclusive as far as the determination of incipient faults is concerned and depends largely on the expertise of technical personnel. To have a coherent, accurate, and clear interpretation of DGA, this study proposes a novel multinomial classification model christened KosaNet that is based on decision trees. Actual DGA data with 2912 entries was used to compute the performance of KosaNet against other algorithms with multiclass classification ability namely the decision tree, k-NN, Random Forest, Naïve Bayes, and Gradient Boost. Investigative results show that KosaNet demonstrated an improved DGA classification ability particularly when classifying multinomial data.
Exemplification of Detecting Gas Turbine Blade Structure Defects Using the X-ray Computed Tomography Method
Authors: Błachnio Józef , Chalimoniuk Marek , Kułaszka Artur , Borowczyk Henryk , Zasada Dariusz
Published: April 2021

X-ray computed tomography is more often applied in non-destructive testing the quality of construction elements significantly crucial for reliability and safety of device elements, machines and complex industrial systems. This article describes the computed tomography (CT) system used to inspect the technical condition of turbine blades of the aircraft engine. The impact of the experimental conditions on the correctness of the obtained results was determined. The appropriate selection of parameters for the experiment was given, and the correct test results of gas turbine blades were presented. Failures, manufacturing defects, material deviations of nickel-cobalt alloyed blades were identified. The thickness of walls was measured in the selected cross-sections with the accuracy of 0.01 mm, and selected manufacturing defects of cooling passages were diagnosed. It was demonstrated that the application of the CT system allows for detailed non-destructive inspection of the technical condition of machine parts. The test results proved that the X-ray computed tomography could be applied in the production and repairs of machines.
Multi-Site Evaluation of Accumulated Temperature and Rainfall for Maize Yield and Disease in Loess Plateau
Authors: Wang Xiaoyue , Zhang Xinghua , Yang Mingxian , Gou Xiaonan , Liu Binbin , Hao Yinchuan , Xu Shutu , Xue Jiquan , Qin Xiaoliang , Siddique Kadambot H. M.
Published: April 2021

The Guanzhong region is a typical and important grain-producing area in China. The effect of accumulated temperature and rainfall on maize production is important in the face of global warming. Here, we collected meteorological data from six test sites in the Guanzhong region to study climate change from 1972 to 2018 in this area. A two-year study was conducted at multiple experimental sites to analyze the effect of climatic factors on maize yield and disease in the Guanzhong region. In the past 40 years, average temperatures have significantly increased at all sites, except for Hancheng. Rainfall varied significantly between years at each site, except for Huxian, with an overall declining trend. Accumulated temperature had a significant positive effect on yield (R2 = 0.28, p = 0.041 < 0.05), but rainfall did not affect yield (R2 = 0.0971, p = 0.324 > 0.05). During the growing period, total rainfall had a significant positive correlation with northern leaf blight disease in maize, and rainfall before silking had a significant positive correlation with ear length and row grain number. The demand for accumulated temperature by maize differed between sites. It is predicted that maize yield will increase with increasing temperature in the Guanzhong region. Greater attention should be paid to improve agronomic practices, such as adjustment of sowing dates, straw mulching, deep tillage, and pest control to adapt to future climate change.
Identification and Analysis of Strawberries’ Consumer Opinions on Twitter for Marketing Purposes
Authors: Borrero Juan D. , Zabalo Alberto
Published: April 2021

Data are currently characterized as the world’s most valuable resource and agriculture is responding to this global trend. The challenge in that particular field of study is to create a Digital Agriculture that help the agri-food sector grow in a fair, competitive environment. As automated machine learning techniques and big data are global research trends in agronomy, this paper aims at comparing different marketing techniques based on Content Analysis to determine the feasibility of using Twitter to design marketing strategies and to determine which techniques are more effective, in particular, for the strawberry industry. A total of 2249 hashtags were subjected to Content Analysis using the Word-count technique, Grounded Theory Method (GTM), and Network Analysis (NA). Findings confirm the results of previous studies regarding Twitter’s potential as a useful source of information due to its lower execution and analysis costs. In general, NA is more effective, cheaper, and faster for Content Analysis than that based both on GTM and automated Word-count. This paper reveals the potential of strawberry-related Twitter data for conducting berry consumer studies, useful in increasing the competitiveness of the berry sector and filling an important gap in the literature by providing guidance on the challenge of data science in agronomy.
Is There a Link between Suckling and Manipulation Behavior during Rearing in Pigs?
Authors: Warns Friederike K. , Gültas Mehmet , van Asten Astrid L. , Scholz Tobias , Gerken Martina
Published: April 2021

Inadequate possibilities to perform oral manipulation behavior for pigs can lead to misdirection and thus tail biting. Our study aimed to analyze manipulation behaviors of weaner pigs with focus on tail biting and the relationship with agonistic characteristics of the piglets during suckling. We analyzed the individual manipulation behavior of 188 weaner pigs. General health condition and tail lesions were determined weekly. Correlations were estimated between weight at weaning and at the end of rearing period, frequency of manipulative rearing behaviors and Dominance and social tension index based on suckling behavior. Principal component and cluster analyses were performed to identify groups of piglets which showed similar suckling and rearing behaviors. Tail biting increased at the middle and end of rearing with switching roles of biters and victims. Tail lesions were correlated with received tail biting behavior but occurred with a delay of more than a week. The frequency of performed tail biting was correlated with dominance index (rs = −0.256, p < 0.01) and weaning weight (rs = −0.199, p < 0.05). We assume that performed tail biting is more often observed in pigs who show mainly submissive behavior in teat disputes.