bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Fluid Intake on Acute Changes in Plasma Volume: A Randomized Controlled Crossover Pilot Trial
Authors: Schierbauer Janis , Sanfilippo Sabrina , Grothoff Auguste , Fehr Ulrich , Wachsmuth Nadine , Voit Thomas , Zimmermann Paul , Moser Othmar
Published: May 2024

Plasma volume (PV) undergoes constant and dynamic changes, leading to a large intra-day variability in healthy individuals. Hydration is known to induce PV changes; however, the response to the intake of osmotically different fluids is still not fully understood. In a randomized controlled crossover trial, 18 healthy individuals (10 females) orally received an individual amount of an isotonic sodium-chloride (ISO), Ringer (RIN), or glucose (GLU) solution. Hemoglobin mass (Hbmass) was determined with the optimized carbon monoxide re-breathing method. Fluid-induced changes in PV were subsequently calculated based on capillary hemoglobin concentration ([Hb]) and hematocrit (Hct) before and then every 10 minutes until 120 min (t0–120) after the fluid intake and compared to a control trial arm (CON), where no fluid was administered. Within GLU and CON trial arms, no statistically significant differences from baseline until t120 were found (p > 0.05). In the ISO trial arm, PV was significantly increased at t70 (+138 mL, p = 0.01), t80 (+191 mL, p < 0.01), and t110 (+182 mL, p = 0.01) when compared to t0. Moreover, PV in the ISO trial arm was significantly higher at t70 (p = 0.02), t110 (p = 0.04), and t120 (p = 0.01) when compared to the same time points in the CON trial arm. Within the RIN trial arm, PV was significantly higher between t70 and t90 (+183 mL, p = 0.01) and between t110 (+194 mL, p = 0.03) and t120 (+186 mL, p < 0.01) when compared to t0. These results demonstrated that fluids with a higher content of osmotically active particles lead to acute hemodilution, which is associated with a decrease in [Hb] and Hct. These findings underpin the importance of the hydration state on PV and especially on PV constituent levels in healthy individuals.
Effects of Pre-Stretching Amount on Motion Characteristics and Screening Efficiency of Flip-Flow Screen Plates
Authors: Wang Yixin , Geng Runhui , Yu Wanqing , Lin Dongdong , Wang Ziqian , Wang Xinwen
Published: May 2024

This study systematically investigates the impact of pre-stretching amount (PSA) on the acceleration, motion status, and screening efficiency of the Flip-flow screen plate (FFSP). Initially, a nonlinear spring-multi-body model of the FFSP is established. Subsequently, the acceleration signals at the midpoint of the FFSP under various PSAs are measured and analyzed. The nonlinear stiffness coefficient of FFSP along the vertical direction is then determined and it is found that simplifying the nonlinear spring-multi-body system to a nonlinear spring-tri-body system under the experimental conditions can maintain the calculation error of FFSP’s acceleration within 30%. Phase and Poincaré mapping diagrams of the FFSP under different PSAs are subsequently created to illustrate the impact of PSA on the motion status of the FFSP. Finally, screening experiments are performed to study the optimal PSA for a kind of bituminous coal from Shanxi province.
Measurements of Thermodynamic Data of Water in Ca-Bentonite by Relative Humidity Method
Authors: Ichikawa Kosuke , Sato Haruo
Published: May 2024

Buffer material (compacted bentonite), one of the engineered barrier elements in the geological disposal of a high-level radioactive waste, develops swelling stress due to groundwater penetration from the surrounding rock mass. Montmorillonite is the major clay mineral component of bentonite. Even previous studies provide few mechanical and thermodynamic data on Ca-montmorillonite. In this study, thermodynamic data on Ca-montmorillonite were obtained as a function of water content by measuring relative humidity (RH) and temperature. The activities of water and the relative partial molar Gibbs free energies of water were determined from the experimental results, and the swelling stress of Ca-bentonite was calculated using the thermodynamic model and compared with measured data. The activities of water and the relative partial molar Gibbs free energies obtained in the experiments decreased with decreasing water content in water contents lower than about 25%. This trend was similar to that of Na-montmorillonite. The swelling stress calculated based on the thermodynamic model was approximately 200 MPa at a montmorillonite partial density of 2.0 Mg/m3 and approximately 10 MPa at a montmorillonite partial density of 1.4 Mg/m3. The swelling stresses in the high-density region (around 2.0 Mg/m3) were higher than that of Na-montmorillonite and were similar levels in the low-density region (around 1.5 Mg/m3). Comparison with measured data showed the practicality of the thermodynamic model.
Nature of Perovskite Mineralization of Silicate-Carbonate Veins in the Margins of Kusinsko-Kopanskaya Layered Intrusion (South Urals, Russia)
Authors: Stepanov Sergey , Palamarchuk Roman , Kutyrev Anton , Lepekhina Elena , Sharpenok Ludmila , Shagalov Evgeniy , Minervina Elena
Published: May 2024

This study presents the first comprehensive investigation of perovskite from its type locality (Mineral Mines of Southern Urals, Russia), where this mineral was first described by Gustav Rose in 1839. The new data includes results from precise chemical analyses (electron-probe microanalyzer, LA-ICP-MS) and U-Pb ages (SHRIMP-II) of perovskite. Perovskite occurs in silicate-carbonate veins that transect the marginal parts of the Middle Riphaean Kusinsko-Kopanskaya layered intrusion, previously thought to be skarns. The perovskite crystals range from micrometer-scale grains to up to 11 cm in size. Chemical investigations revealed a low content of trace elements (rare earth elements, Y, Nd, U, Th) compared to perovskites from alkaline ultramafic rocks, silica-undersaturated basic rocks, carbonatites, and kimberlites. The determined age of the perovskite, 535 ± 43 Ma, significantly differs from the 1379 ± 8 Ma age of the Kusinsko-Kopanskaya intrusion, challenging the skarn-origin hypothesis for perovskite. Instead, the findings suggest a carbonatite origin for the perovskite mineralization. This timing indicates a previously unknown stage of endogenic activity on the Western Slope of the Southern Urals.
Lithofacies Characteristics of Continental Lacustrine Fine-Grained Sedimentary Rocks and Their Coupling Relationship with Sedimentary Environments: Insights from the Shahejie Formation, Dongying Sag
Authors: Guo Hao , Shi Juye , Fu Shaopeng , Liu Zitong , Cai Linhong , Yin Siyuan
Published: May 2024

Lacustrine fine-grained sedimentary rocks in the Dongying Sag of the Bohai Bay Basin in China exhibit significant potential for hydrocarbon exploration. This study investigates the lithofacies types and sedimentary evolution of the Paleogene Shahejie Formation’s lower third member (Es3l) and upper fourth member (Es4u), integrating petrological and geochemical analyses to explore the relationship between lithofacies characteristics and sedimentary environments. The results show that the fine-grained sedimentary rocks in the study area can be classified into 18 lithofacies, with seven principal ones, including organic-rich laminated carbonate fine-grained mixed sedimentary rock lithofacies and organic-rich laminated limestone lithofacies. In conjunction with analyses of vertical changes in geochemical proxies such as paleoclimate (e.g., CIA, Na/Al), paleoproductivity (e.g., Ba), paleosalinity (e.g., Sr/Ba), paleo-redox conditions (e.g., V/Sc, V/V + Ni), and terrigenous detrital influx (e.g., Al, Ti), five stages are delineated from bottom to top. These stages demonstrate a general transition from an arid to humid paleoclimate, a steady increase in paleoproductivity, a gradual decrease in paleosalinity, an overall reducing water body environment, and an increasing trend of terrestrial detrital input. This study demonstrates that the abundance of organic matter is primarily influenced by paleoproductivity and paleo-redox conditions. The variations in rock components are predominantly influenced by paleoclimate, and sedimentary structures are affected by the depth of the lake basin. Special depositional events, such as storm events in Stage II, have significantly impacted the abundance of organic matter, rock components, and sedimentary structures by disturbing the water column and disrupting the reducing conditions at the lake bottom. The present study offers crucial insights into the genesis mechanisms of continental lacustrine fine-grained sedimentary rocks, facilitates the prediction of lithofacies distribution, and advances the exploration of China’s shale oil resources in lacustrine environments.
Are Clay Minerals Systematically the Products of Aqueous Alteration in Cosmic Bodies?
Authors: El Albani Abderrazak , Chraiki Ibtissam , Aoudjehane Hasnaa , Ghnahalla Mohamed , Abdelfadel Fatima , Elmola Ahmed , Bankole Olabode , Ikouanga Julie , El Khoury Anna , Fontaine Claude , Bouougri El , Westall France , Meunier Alain
Published: May 2024

The formation of chondrite materials represents one of the earliest mineralogical processes in the solar system. Phyllosilicates are encountered at various stages of the chondrule formation, from the initial stages (IDP agglomerates) to the final steps (chondrule internal alteration). While typically linked to aqueous alteration, recent studies reveal that phyllosilicates could precipitate directly from residual fluids in post-magmatic or deuteric conditions and under a wide range of temperatures, pressures, water/rock ratios, and H2/H2O ratio conditions. This study re-examined the formation of hydrated phyllosilicates in chondrules and associated fine-grained rims (FGRs) using published petrographical, mineralogical, and chemical data on carbonaceous chondrites. Given that chondrules originate from the melting of interplanetary dust particles, the water liberated by the devolatilization of primary phyllosilicates, including clay minerals or ice melting, reduces the melting temperature and leads to water dissolution into the silicate melt. Anhydrous minerals (e.g., olivine and diopside) form first, while volatile and incompatible components are concentrated in the residual liquid, diffusing into the matrix and forming less porous FGRs. Serpentine and cronstedtite are the products of thermal metamorphic-like mineral reactions. The mesostasis in some lobated chondrules is composed of anhydrous and hydrous minerals, i.e., diopside and serpentine. The latter is probably not the alteration product of a glassy precursor but rather a symplectite component (concomitant crystallization of diopside and serpentine). If so, the symplectite has been formed at the end of the cooling process (eutectic-like petrographical features). Water trapped inside chondrule porosity can lead to the local replacement of olivine by serpentine without external water input (auto-alteration). In the absence of water, hydrated phyllosilicates do not crystallize, forming a different mineral assemblage.
Changes in the Glucose Concentration Affect the Formation of Humic-like Substances in Polyphenol–Maillard Reactions Involving Gibbsite
Authors: Wang Nan , Cui Yongquan , Zhou Yanhui , Liu Pingxin , Wang Mingshuo , Sun Haihang , Huang Yubao , Wang Shuai
Published: May 2024

The polyphenol–Maillard reaction is considered one of the important pathways in the formation of humic-like substances (HLSs). Glucose serves as a microbial energy source that drives the humification process. However, the effects of changes in glucose, particularly its concentration, on abiotic pathways remain unclear. Given that the polyphenol–Maillard reaction requires high precursor concentrations and elevated temperatures (which are not present in soil), gibbsite was used as a catalyst to overcome energetic barriers. Catechol and glycine were introduced in fixed concentrations into a phosphate-buffered solution containing gibbsite using the liquid shake-flask incubation method, while the concentration of glucose was controlled in a sterile incubation system. The supernatant fluid and HLS components were dynamically extracted over a period of 360 h for analysis, thus revealing the influence of different glucose concentrations on abiotic humification pathways. The results showed the following: (1) The addition of glucose led to a higher degree of aromatic condensation in the supernatant fluid. In contrast, the supernatant fluid without glucose (Glu0) and the control group without any Maillard precursor (CK control group) exhibited lower degrees of aromatic condensation. Although the total organic C (TOC) content in the supernatant fluid decreased in all treatments during the incubation period, the addition of Maillard precursors effectively mitigated the decreasing trend of TOC content. (2) While the C content of humic-like acid (CHLA) and the CHLA/CFLA ratio (the ratio of humic-like acid to fulvic-like acid) showed varying increases after incubation, the addition of Maillard precursors resulted in a more noticeable increase in CHLA content and the CHLA/CFLA ratio compared to the CK control group. This indicated that more FLA was converted into HLA, which exhibited a higher degree of condensation and humification, thus improving the quality of HLS. The addition of glycine and catechol without glucose or with a glucose concentration of 0.06 mol/L was particularly beneficial in enhancing the degree of HLA humification. Furthermore, the presence of glycine and catechol, as well as higher concentrations of glucose, promoted the production of N-containing compounds in HLA. (3) The presence of Maillard precursors enhanced the stretching vibration of the hydroxyl group (–OH) of HLA. After the polyphenol–Maillard reaction of glycine and catechol with glucose concentrations of 0, 0.03, 0.06, 0.12, or 0.24 mol/L, the aromatic C structure in HLA products increased, while the carboxyl group decreased. The presence of Maillard precursors facilitated the accumulation of polysaccharides in HLA with higher glucose concentrations, ultimately promoting the formation of Al–O bonds. However, the quantities of phenolic groups and phenols in HLA decreased to varying extents.
Zinc N,N-bis(2-picolyl)amine Chelates Show Substitution-Dependent Cleavage of Phosphodiesters in Models as Well as of PNAzyme-RNA Bulges
Authors: Svenningsen Søren W. , Luige Olivia , Abdulkarim Zeyed , Strömberg Roger , Williams Nicholas H.
Published: May 2024

PNAzymes are a group of artificial enzymes which show promising results in selective and efficient cleavage of RNA targets. In the present study, we introduce a series of metal chelating groups based on N,N-bis(2-picolyl) groups (parent, 6-methyl and 6-amino substituted) as the active sites of novel PNAzymes. An improved synthetic route for the 6-amino analogues is described. The catalytic activity of the chelating groups for cleaving phosphodiesters were assessed with the model substrate 2-hydroxypropyl p-nitrophenyl phosphate (HPNPP), confirming that the zinc complexes have the reactivity order of parent < 2-methyl < 2-amino. The three ligands were conjugated to a PNA oligomer to form three PNAzymes which showed the same order of reactivity and some sensitivity to the size of the RNA bulge designed into the catalyst–substrate complex. This work demonstrates that the kinetic activity observed for the model substrate HPNPP could be translated onto the PNAzymes, but that more reactive Zn complexes are required for such PNAzymes to be viable therapeutic agents.
Enhanced Mass Activity and Durability of Bimetallic Pt-Pd Nanoparticles on Sulfated-Zirconia-Doped Graphene Nanoplates for Oxygen Reduction Reaction in Proton Exchange Membrane Fuel Cell Applications
Authors: Yaldagard Maryam , Arkas Michael
Published: May 2024

Developing highly active and durable Pt-based electrocatalysts is crucial for polymer electrolyte membrane fuel cells. This study focuses on the performance of oxygen reduction reaction (ORR) electrocatalysts composed of Pt-Pd alloy nanoparticles on graphene nanoplates (GNPs) anchored with sulfated zirconia nanoparticles. The results of field emission scanning electron microscopy and transmission electron microscopy showed that Pt-Pd and S-ZrO2 are well dispersed on the surface of the GNPs. X-ray diffraction revealed that the S-ZrO2 and Pt-Pd alloy coexist in the Pt-Pd/S-ZrO2-GNP nanocomposites without affecting the crystalline lattice of Pt and the graphitic structure of the GNPs. To evaluate the electrochemical activity and reaction kinetics for ORR, we performed cyclic voltammetry, rotating disc electrode, and EIS experiments in acidic solutions at room temperature. The findings showed that Pt-Pd/S-ZrO2-GNPs exhibited a better ORR performance than the Pt-Pd catalyst on the unsulfated ZrO2-GNP support and with Pt on S-ZrO2-GNPs and commercial Pt/C.
Integrated Approach of Life Cycle Assessment and Experimental Design in the Study of a Model Organic Reaction: New Perspectives in Renewable Vanillin-Derived Chemicals
Authors: Ruini Chiara , Ferrari Erika , Durante Caterina , Lanciotti Giulia , Neri Paolo , Ferrari Anna Maria , Rosa Roberto
Published: May 2024

This work is focused on performing a quantitative assessment of the environmental impacts associated with an organic synthesis reaction, optimized using an experimental design approach. A nucleophilic substitution reaction was selected, employing vanillin as the substrate, a phenolic compound widely used in the food industry and of pharmaceutical interest, considering its antioxidant and antitumoral potential. To carry out the reaction, three different solvents have been chosen, namely acetonitrile (ACN), acetone (Ace), and dimethylformamide (DMF). The syntheses were planned with the aid of a multivariate experimental design to estimate the best reaction conditions, which simultaneously allow a high product yield and a reduced environmental impact as computed by Life Cycle Assessment (LCA) methodology. The experimental results highlighted that the reactions carried out in DMF resulted in higher yields with respect to ACN and Ace; these reactions were also the ones with lower environmental impacts. The multilinear regression models allowed us to identify the optimal experimental conditions able to guarantee the highest reaction yields and lowest environmental impacts for the studied reaction. The identified optimal experimental conditions were also validated by experimentally conducting the reaction in those conditions, which indeed led to the highest yield (i.e., 93%) and the lowest environmental impacts among the performed experiments. This work proposes, for the first time, an integrated approach of DoE and LCA applied to an organic reaction with the aim of considering both conventional metrics, such as reaction yield, and unconventional ones, such as environmental impacts, during its lab-scale optimization.
Chromium Catalysts for Selective Ethylene Oligomerization Featuring Binuclear PNP Ligands
Authors: Meng Xiangyang , Ding Zhiqiang , Gao Huan , Ma Zhe , Pan Li , Wang Bin , Li Yuesheng
Published: May 2024

A series of novel binuclear PNP ligands based on the cyclohexyldiamine scaffold were synthesized for this study. The experimental results showed that positioning the two PNP sites at the para-positions of the cyclohexyl framework led to a significant enhancement in the catalytic activity for selective tri/tetramerization of ethylene. The PNP/Cr(acac)3/MAO(methylaluminoxane) catalytic system exhibited relatively high catalytic activity (up to 3887.7 kg·g−1·h−1) in selective ethylene oligomerization with a total selectivity of 84.5% for 1-hexene and 1-octene at 40 °C and 50 bar. The relationship between the ligand structure and ethylene oligomerization performance was further explored using density functional theory calculations.
Metal–Organic Framework Nanomaterials as a Medicine for Catalytic Tumor Therapy: Recent Advances
Authors: Zhang Jiaojiao , Li Meiyu , Liu Maosong , Yu Qian , Ge Dengfeng , Zhang Jianming
Published: May 2024

Nanomaterials, with unique physical, chemical, and biocompatible properties, have attracted significant attention as an emerging active platform in cancer diagnosis and treatment. Amongst them, metal–organic framework (MOF) nanostructures are particularly promising as a nanomedicine due to their exceptional surface functionalities, adsorption properties, and organo-inorganic hybrid characteristics. Furthermore, when bioactive substances are integrated into the structure of MOFs, these materials can be used as anti-tumor agents with superior performance compared to traditional nanomaterials. In this review, we highlight the most recent advances in MOFs-based materials for tumor therapy, including their application in cancer treatment and the underlying mechanisms.
Na3MnTi(PO4)3/C Nanofiber Free-Standing Electrode for Long-Cycling-Life Sodium-Ion Batteries
Authors: Conti Debora Maria , Urru Claudia , Bruni Giovanna , Galinetto Pietro , Albini Benedetta , Berbenni Vittorio , Girella Alessandro , Capsoni Doretta
Published: May 2024

Self-standing Na3MnTi(PO4)3/carbon nanofiber (CNF) electrodes are successfully synthesized by electrospinning. A pre-synthesized Na3MnTi(PO4)3 is dispersed in a polymeric solution, and the electrospun product is heat-treated at 750 °C in nitrogen flow to obtain active material/CNF electrodes. The active material loading is 10 wt%. SEM, TEM, and EDS analyses demonstrate that the Na3MnTi(PO4)3 particles are homogeneously spread into and within CNFs. The loaded Na3MnTi(PO4)3 displays the NASICON structure; compared to the pre-synthesized material, the higher sintering temperature (750 °C) used to obtain conductive CNFs leads to cell shrinkage along the a axis. The electrochemical performances are appealing compared to a tape-casted electrode appositely prepared. The self-standing electrode displays an initial discharge capacity of 124.38 mAh/g at 0.05C, completely recovered after cycling at an increasing C-rate and a coulombic efficiency ≥98%. The capacity value at 20C is 77.60 mAh/g, and the self-standing electrode exhibits good cycling performance and a capacity retention of 59.6% after 1000 cycles at 1C. Specific capacities of 33.6, 22.6, and 17.3 mAh/g are obtained by further cycling at 5C, 10C, and 20C, and the initial capacity is completely recovered after 1350 cycles. The promising capacity values and cycling performance are due to the easy electrolyte diffusion and contact with the active material, offered by the porous nature of non-woven nanofibers.
A Systematic Review and Meta-Analysis of microRNA Profiling Studies in Chronic Kidney Diseases
Authors: Garmaa Gantsetseg , Bunduc Stefania , Kói Tamás , Hegyi Péter , Csupor Dezső , Ganbat Dariimaa , Dembrovszky Fanni , Meznerics Fanni Adél , Nasirzadeh Ailar , Barbagallo Cristina , Kökény Gábor
Published: May 2024

Chronic kidney disease (CKD) represents an increasing health burden. Evidence suggests the importance of miRNA in diagnosing CKD, yet the reports are inconsistent. This study aimed to determine novel miRNA biomarkers and potential therapeutic targets from hypothesis-free miRNA profiling studies in human and murine CKDs. Comprehensive literature searches were conducted on five databases. Subgroup analyses of kidney diseases, sample types, disease stages, and species were conducted. A total of 38 human and 12 murine eligible studies were analyzed using Robust Rank Aggregation (RRA) and vote-counting analyses. Gene set enrichment analyses of miRNA signatures in each kidney disease were conducted using DIANA-miRPath v4.0 and MIENTURNET. As a result, top target genes, Gene Ontology terms, the interaction network between miRNA and target genes, and molecular pathways in each kidney disease were identified. According to vote-counting analysis, 145 miRNAs were dysregulated in human kidney diseases, and 32 were dysregulated in murine CKD models. By RRA, miR-26a-5p was significantly reduced in the kidney tissue of Lupus nephritis (LN), while miR-107 was decreased in LN patients’ blood samples. In both species, epithelial-mesenchymal transition, Notch, mTOR signaling, apoptosis, G2/M checkpoint, and hypoxia were the most enriched pathways. These miRNA signatures and their target genes must be validated in large patient cohort studies.
Blood Lead Level as Marker of Increased Risk of Ovarian Cancer in BRCA1 Carriers
Authors: Kiljańczyk Adam , Matuszczak Milena , Marciniak Wojciech , Derkacz Róża , Stempa Klaudia , Baszuk Piotr , Bryśkiewicz Marta , Lubiński Krzysztof , Cybulski Cezary , Dębniak Tadeusz , Gronwald Jacek , Huzarski Tomasz , Lener Marcin R. , Jakubowska Anna , Szwiec Marek , Stawicka-Niełacna Małgorzata , Godlewski Dariusz , Prusaczyk Artur , Jasiewicz Andrzej , Kluz Tomasz , Tomiczek-Szwiec Joanna , Kilar-Kobierzycka Ewa , Siołek Monika , Wiśniowski Rafał , Posmyk Renata , Jarkiewicz-Tretyn Joanna , Sun Ping , Scott Rodney J. , Narod Steven A. , Lubiński Jan
Published: May 2024

BRCA1 mutations substantially elevate the risks of breast and ovarian cancer. Various modifiers, including environmental factors, can influence cancer risk. Lead, a known carcinogen, has been associated with various cancers, but its impact on BRCA1 carriers remains unexplored. A cohort of 989 BRCA1 mutation carriers underwent genetic testing at the Pomeranian Medical University, Poland. Blood lead levels were measured using inductively coupled plasma mass spectrometry. Each subject was assigned to a category based on their tertile of blood lead. Cox regression analysis was used to assess cancer risk associations. Elevated blood lead levels (>13.6 μg/L) were associated with an increased risk of ovarian cancer (univariable: HR = 3.33; 95% CI: 1.23–9.00; p = 0.02; multivariable: HR = 2.10; 95% CI: 0.73–6.01; p = 0.17). No significant correlation was found with breast cancer risk. High blood lead levels are associated with increased risk of ovarian cancer in BRCA1 carriers, suggesting priority for preventive salpingo-oophorectomy. Potential risk reduction strategies include detoxification. Validation in diverse populations and exploration of detoxification methods for lowering lead levels are required.
Cholesterol and Its Oxidation Derivatives Content in Market Dairy Products
Authors: Czerwonka Małgorzata , Gielecińska Anna , Białek Agnieszka , Białek Małgorzata , Bobrowska-Korczak Barbara
Published: May 2024

Cholesterol oxidation products (COPs) are contaminants of food of animal origin. Increased levels of these compounds in the human body are associated with an increased risk of many non-communicable diseases. Dairy products are mentioned among the main sources of these compounds in the diet. The objective of this study was to evaluate the contents of cholesterol and its oxidized derivatives in eleven groups of dairy products, willingly consumed in European countries. The levels of COPs were determined by applying the GC-TOF/MS method. In the tested products, cholesterol and its oxidation derivatives, such as 7-ketocholesterol, 7α-hydroxycholesterol, 7β-hydroxycholesterol, 5,6β-epoxycholesterol and 5,6α-epoxycholesterol, were determined. The studied dairy products differed in their contents and profiles of oxysterols. The highest contents of COPs were found in cheese with internal mold (13.8 ± 2.5 mg kg−1) and Cheddar (11.7 ± 3.5 mg kg−1), while the lowest levels were detected in yoghurt (0.94 ± 0.30 mg kg−1) and kefir (0.57 ± 0.11 mg kg−1). 7-ketocholesterol and 5,6β-epoxycholesterol were the dominant oxysterols. The ratio of oxidized derivatives to total cholesterol was on average 1.7%. Our results confirmed that dairy products are an important dietary source of COPs. Their levels should be monitored in dairy products to provide the best health quality.
Effects of a Personalized Diet on Nutritional Status and Renal Function Outcome in Nephrectomized Patients with Renal Cancer
Authors: Trevisani Francesco , Laurenti Fabiana , Fiorio Francesco , Paccagnella Matteo , Floris Matteo , Capitanio Umberto , Ghidini Michele , Garrone Ornella , Abbona Andrea , Salonia Andrea , Montorsi Francesco , Bettiga Arianna
Published: May 2024

Nutritional therapy (NT) based on a controlled protein intake represents a cornerstone in managing chronic kidney disease (CKD). However, if a CKD patient is at the same time affected by cancer, oncologists and nutritionists tend to suggest a dietary regimen based on high protein intake to avoid catabolism and malnutrition. International guidelines are not clear when we consider onco-nephrological patients and, as a consequence, no clinical shared strategy is currently applied in clinical practice. In particular, no precise nutritional management is established in nephrectomized patients for renal cell carcinoma (RCC), a specific oncological cohort of patients whose sudden kidney removal forces the remnant one to start a compensatory mechanism of adaptive hyperfiltration. Our study aimed to investigate the efficacy of a low–normal-protein high-calorie (LNPHC) diet based on a Mediterranean model in a consecutive cohort of nephrectomized RCC patients using an integrated nephrologist and nutritionist approach. A consecutive cohort of 40 nephrectomized RCC adult (age > 18) patients who were screened for malnutrition (malnutrition screening tool, MST < 2) were enrolled in a tertiary institution between 2020 and 2022 after signing a specific informed consent form. Each patient underwent an initial nephrological and nutritional evaluation and was subsequently subjected to a conventional CKD LNPHC diet integrated with aproteic foods (0.8 g/Kg/die: calories: 30–35 kcal per kg body weight/die) for a period of 6 months (±2 months). The diet was structured after considering eGFR (CKD-EPI 2021 creatinine formula), comorbidities, and nutritional status. MST, body mass index (BMI), phase angle (PA), fat mass percentage (FM%), fat-free mass index (FFMI), body cell mass index (BCMI), extracellular/intracellular water ratio (ECW/ICW), extracellular matrix/body cell mass ratio (ECM/BCM), waist/hip circumference ratio (WHC), lab test exams, and clinical variables were examined at baseline and after the study period. Our results clearly highlighted that the LNPHC diet was able to significantly improve several nutritional parameters, avoiding malnutrition and catabolism. In particular, the LNPHC diet preserved the BCM index (delta on median, ΔM + 0.3 kg/m2) and reduced the ECM/BCM ratio (ΔM − 0.03 *), with a significant reduction in the ECW/ICW ratio (ΔM − 0.02 *), all while increasing TBW (ΔM + 2.3% *). The LNPHC diet was able to preserve FFM while simultaneously depleting FM and, moreover, it led to a significant reduction in urea (ΔM − 11 mg/dL **). In conclusion, the LNPHC diet represents a new important therapeutic strategy that should be considered when treating onco-nephrological patients with solitary kidney due to renal cancer.
Mechanical Properties of Direct Composite Resins and CAD/CAM Composite Blocks
Authors: Ramos João Carlos , Marinho Alfredo , Messias Ana , Almeida Gabriela , Vinagre Alexandra , Dias Ricardo
Published: May 2024

The widespread application of CAD/CAM technology in contemporary dentistry led to the development of promising restorative materials, such as resin composite blocks (RCBs). Thus, the present study aims to evaluate the mechanical properties of RCBs, comparing this material to the direct composite resin from the same manufacturer. Samples retrieved from three CAD/CAM resin composite blocks (Tetric CAD (TC), Ivoclar Vivadent, Grandio blocs (GB), VOCO GmbH and Brilliant Crios (BC), Coltene/Whaledent) and four direct composite resins (Tetric EvoCeram (TEC), Ivoclar Vivadent, GrandioSO (GS), VOCO GmbH, Brilliant EverGlow Translucent (BET) and Universal Shade (BEU), Coltene/Whaledent) were submitted to three-point bending flexural test and Vickers microhardness test. The resulting data of the flexural strength were analyzed using one-way ANOVA considering Bonferroni correction for post hoc tests (α = 0.05). The flexural modulus and Vickers microhardness results were analyzed using Welch’s ANOVA considering Games–Howell correction for post hoc tests (α = 0.05). Regarding results, flexural strength and flexural modulus values ranged from 81.1 MPa (BEU) to 246.5 MPa (GB) and 10.6 GPa (BEU) to 20.3 GPa (GB), respectively. GS (121.2) and GB (136.2) groups were associated with the highest microhardness values. According to the post hoc tests, statistically significant differences in flexure strength were found in RCBs (BC, GB, and TC) compared to all direct composite resins. Flexural modulus and Vickers microhardness of RCBs (BC, GB, TC) were also significantly different from the direct composite resin (BET, BED, and TEC), except when comparing GS and GB for microhardness. In conclusion, differences between RCBs and direct composite resins were observed regarding flexural strength, flexural modulus, and microhardness, revealing that RCBs have enhanced mechanical properties compared to direct composite resins.
Amber (Succinite) Extract Enhances Glucose Uptake through the Up-Regulation of ATP and Down-Regulation of ROS in Mouse C2C12 Cells
Authors: Othman Mahmoud Ben , Takeda Reiko , Sekita Marie , Okazaki Kazuma , Sakamoto Kazuichi
Published: May 2024

Traditionally, amber (Succinite) has been used to alleviate all types of pain, skin allergies, and headaches. However, no studies have been conducted on its antidiabetic and antioxidant effects. In this study, differentiated skeletal muscle C2C12 cells were used to demonstrate the protective effects of amber (AMB) against H2O2-induced cell death. In addition, the effects of AMB on glucose uptake and ATP production were investigated. Our results showed that AMB at 10, 25, and 50 μg/mL suppressed the elevation of ROS production induced by H2O2 in a dose-dependent manner. Moreover, AMB enhanced glucose utilization in C2C12 cells through the improvement of ATP production and an increase in PGC-1α gene expression resulting in an amelioration of mitochondrial activity. On the other hand, AMB significantly increased the gene expression of glucose transporters GLUT4 and GLUT1. Our finding suggests that AMB can be used as a natural supplement for diabetes treatment and for the promotion of skeletal muscle function.
Enhancing Cellular Uptake of Native Proteins through Bio-Orthogonal Conjugation with Chemically Synthesized Cell-Penetrating Peptides
Authors: Nebogatova Jekaterina , Porosk Ly , Härk Heleri Heike , Kurrikoff Kaido
Published: May 2024

The potential for native proteins to serve as a platform for biocompatible, targeted, and personalized therapeutics in the context of genetic and metabolic disorders is vast. Nevertheless, their clinical application encounters challenges, particularly in overcoming biological barriers and addressing the complexities involved in engineering transmembrane permeability. This study is dedicated to the development of a multifunctional nanoentity in which a model therapeutic protein is covalently linked to a cell-penetrating peptide, NickFect 55, with the objective of enhancing its intracellular delivery. Successful binding of the nanoentity fragments was achieved through the utilization of an intein-mediated protein-trans splicing reaction. Our research demonstrates that the fully assembled nanoentity-containing protein was effectively internalized by the cells, underscoring the potential of this approach in overcoming barriers associated with protein-based therapeutics for the treatment of genetic disorders.
Evaluation of Silybin Nanoparticles against Liver Damage in Murine Schistosomiasis mansoni Infection
Authors: Vanzan Daniel Figueiredo , Goma Ester Puna , Locatelli Fernanda Resende , Honorio Thiago da Silva , Furtado Priscila de Souza , Rodrigues Carlos Rangel , de Sousa Valeria Pereira , Mata dos Santos Hilton Antônio , do Carmo Flávia Almada , Simon Alice , Pyrrho Alexandre dos Santos , Ribeiro António José , Cabral Lucio Mendes
Published: May 2024

Silybin (SIB) is a hepatoprotective drug known for its poor oral bioavailability, attributed to its classification as a class IV drug with significant metabolism during the first-pass effect. This study explored the potential of solid lipid nanoparticles with (SLN-SIB-U) or without (SLN-SIB) ursodeoxycholic acid and polymeric nanoparticles (PN-SIB) as delivery systems for SIB. The efficacy of these nanosystems was assessed through in vitro studies using the GRX and Caco-2 cell lines for permeability and proliferation assays, respectively, as well as in vivo experiments employing a murine model of Schistosomiasis mansoni infection in BALB/c mice. The mean diameter and encapsulation efficiency of the nanosystems were as follows: SLN-SIB (252.8 ± 4.4 nm, 90.28 ± 2.2%), SLN-SIB-U (252.9 ± 14.4 nm, 77.05 ± 2.8%), and PN-SIB (241.8 ± 4.1 nm, 98.0 ± 0.2%). In the proliferation assay with the GRX cell line, SLN-SIB and SLN-SIB-U exhibited inhibitory effects of 43.09 ± 5.74% and 38.78 ± 3.78%, respectively, compared to PN-SIB, which showed no inhibitory effect. Moreover, SLN-SIB-U demonstrated a greater apparent permeability coefficient (25.82 ± 2.2) than PN-SIB (20.76 ± 0.1), which was twice as high as that of SLN-SIB (11.32 ± 4.6) and pure SIB (11.28 ± 0.2). These findings suggest that solid lipid nanosystems hold promise for further in vivo investigations. In the murine model of acute-phase Schistosomiasis mansoni infection, both SLN-SIB and SLN-SIB-U displayed hepatoprotective effects, as evidenced by lower alanine amino transferase values (22.89 ± 1.6 and 23.93 ± 2.4 U/L, respectively) than those in control groups I (29.55 ± 0.7 U/L) and I+SIB (34.29 ± 0.3 U/L). Among the prepared nanosystems, SLN-SIB-U emerges as a promising candidate for enhancing the pharmacokinetic properties of SIB.
Cell-Membrane-Coated Metal–Organic Framework Nanocarrier Combining Chemodynamic Therapy for the Inhibition of Hepatocellular Carcinoma Proliferation
Authors: Xie Huaying , Xiao Xuhua , Yi Xiaoyuan , Huang Kunzhao , Wang Liyan
Published: May 2024

Chemodynamic therapy (CDT) employs hydrogen peroxide (H2O2) within the tumor microenvironment (TME) to initiate the Fenton reaction and catalyze the generation of hydroxyl radicals (·OH) for targeted therapy. Metal ion-based nanomaterials have garnered significant attention as catalysts due to their potent anti-tumor effects. Hypoxia in the TME is often associated with cancer cell development and metastasis, with HIF-1α being a pivotal factor in hypoxia adaptation. In this study, an organic framework called MIL-101 (Fe) was designed and synthesized to facilitate H2O2-induced ·OH production while also serving as a carrier for the HIF-1α inhibitor Acriflavine (ACF). A biomimetic nanomedical drug delivery system named MIL-101/ACF@CCM was constructed by encapsulating liver cancer cell membranes onto the framework. This delivery system utilized the homologous targeting of tumor cell membranes to transport ACF, inhibiting HIF-1α expression, alleviating tumor hypoxia, and catalyzing ·OH production for effective tumor eradication. Both in vivo and in vitro experiments confirmed that combining ACF with chemotherapy achieved remarkable tumor inhibition by enhancing ROS production and suppressing HIF-1α expression.
Testosterone Therapy for Late-Onset Hypogonadism: A Clinical, Biological, and Analytical Approach Using Compounded Testosterone 0.5–20% Topical Gels
Authors: Banov Daniel , Biundo Bruce , Ip Kendice , Shan Ashley , Banov Fabiana , Song Guiyun , Carvalho Maria
Published: May 2024

Testosterone is integral to men’s sexual and overall health, but there is a gradual decline in the ageing male. The topical administration of testosterone is a valuable option as a supplement (replacement) therapy to alleviate hypogonadal symptoms. The clinical efficacy of a compounded testosterone 5% topical gel was assessed retrospectively in a male patient in his seventies by evaluating the laboratory testing of the serum total testosterone and the results of a validated androgen deficiency questionnaire. After treatment, the patient’s hypogonadal symptoms improved and the serum total testosterone level achieved was considered clinically optimal. The skin permeation of the testosterone topical gel (biological testing) was evaluated in vitro using the Franz finite dose model and human cadaver skin, and it is shown that testosterone can penetrate into and through ex vivo human skin. Testosterone therapy is often prescribed for extended periods, and consequently, it is crucial to determine the beyond-use date of the compounded formulations. The analytical testing involved a valid, stability-indicating assay method for compounded testosterone 0.5% and 20% topical gels. This multidisciplinary study shows evidence supporting topically applied testosterone’s clinical efficacy and the compounded formulations’ extended stability. Personalized, topical testosterone therapy is a promising alternative in current therapeutics for hypogonadal patients.
Tightly Trapped Atom Interferometer inside a Hollow-Core Fiber
Authors: Song Yitong , Li Wei , Xu Xiaobin , Han Rui , Gao Chengchun , Dai Cheng , Song Ningfang
Published: May 2024

We demonstrate a fiber-guided atom interferometer in a far-off-resonant trap (FORT) of 100 μK. The differential light shift (DLS) introduced by the FORT leads to the inhomogeneous dephasing of the tightly trapped atoms inside a hollow-core fiber. The DLS-induced dephasing is greatly suppressed in π/2-π-π/2 Doppler-insensitive interferometry. The spin coherence time is extended to 13.4 ms by optimizing the coupling of the trapping laser beam into a quasi-single-mode hollow-core anti-resonant fiber. The Doppler-sensitive interferometry shows a much shorter coherence time, indicating that the main limits to our fiber-guided atom interferometer are the wide axial velocity distribution and the irregular modes of the Raman laser beams inside the fiber. This work paves the way for portable and miniaturized quantum devices, which have advantages for inertial sensing at arbitrary orientations and in dynamic environments.
Probing Relativistic Heavy-Ion Collisions via Photon Anisotropic Flow Ratios. A Brief Review
Authors: Chatterjee Rupa , Dasgupta Pingal
Published: May 2024

The anisotropic flow of photons produced in relativistic nuclear collisions is known as a promising observable for studying the initial state and the subsequent evolution of the hot and dense medium formed in such collisions. The investigation of photon anisotropic flow coefficients, vn, has attracted high interest over the last decade, involving both theory and experiment. The thermal emission of photons and their anisotropic flow are found to be highly sensitive to the initial state of the fireball, where even slight modifications can lead to significant variations in the final state results. In contrast, the ratio of photon anisotropic flow stands out as a robust observable, exhibiting minimal sensitivity to the initial conditions. Here, we briefly review the studies of the individual elliptic and triangular flow parameters of photons as well as their ratios and how these parameters serve as valuable probes for investigating the intricacies of the initial state and addressing the challenges posed by the direct photon puzzle.
The Reaction of Rice Growth to the Arsenic Contamination under Various Irrigation Methods
Authors: Szalóki Tímea , Székely Árpád , Valkovszki Noémi J. , Tarnawa Ákos , Jancsó Mihály
Published: May 2024

Several studies have explored how arsenic (As) is absorbed and transported in plants, but less attention has been paid to its impact on rice growth and yield in relation to water management. We aimed to assess how arsenic affects plant development under different irrigation methods. The growth and yield parameters of four rice varieties (‘M 488’, ‘Janka’, ‘Szellő’, and ‘Nembo’) in two greenhouse experiments were analyzed in 2021 and 2022 under different irrigation methods (flooded (F), intermittent (I), and aerobic (A)). Three different As concentrations were set up in the soil: 43 mg kg−1, 24 mg kg−1, and 4 mg kg−1. Our results showed that the high As treatment caused severe damage to the plants including leaf yellowing as well as reduced growth and decreased yield parameters. Alternative water management practices such as I and A irrigation could reduce the negative effects of As. At the high level of As stress (43 mg kg−1), the I irrigation had the most favorable effect on the yield of ’Janka’ among the tested cultivars compared to the F irrigation (in F: 1.64 ± 1.13 g; in I: 5.45 ± 3.69 g). However, the use of fully aerobic conditions increased the likelihood of drought stress.
Ability of Genomic Prediction to Bi-Parent-Derived Breeding Population Using Public Data for Soybean Oil and Protein Content
Authors: Li Chenhui , Yang Qing , Liu Bingqiang , Shi Xiaolei , Liu Zhi , Yang Chunyan , Wang Tao , Xiao Fuming , Zhang Mengchen , Shi Ainong , Yan Long
Published: May 2024

Genomic selection (GS) is a marker-based selection method used to improve the genetic gain of quantitative traits in plant breeding. A large number of breeding datasets are available in the soybean database, and the application of these public datasets in GS will improve breeding efficiency and reduce time and cost. However, the most important problem to be solved is how to improve the ability of across-population prediction. The objectives of this study were to perform genomic prediction (GP) and estimate the prediction ability (PA) for seed oil and protein contents in soybean using available public datasets to predict breeding populations in current, ongoing breeding programs. In this study, six public datasets of USDA GRIN soybean germplasm accessions with available phenotypic data of seed oil and protein contents from different experimental populations and their genotypic data of single-nucleotide polymorphisms (SNPs) were used to perform GP and to predict a bi-parent-derived breeding population in our experiment. The average PA was 0.55 and 0.50 for seed oil and protein contents within the bi-parents population according to the within-population prediction; and 0.45 for oil and 0.39 for protein content when the six USDA populations were combined and employed as training sets to predict the bi-parent-derived population. The results showed that four USDA-cultivated populations can be used as a training set individually or combined to predict oil and protein contents in GS when using 800 or more USDA germplasm accessions as a training set. The smaller the genetic distance between training population and testing population, the higher the PA. The PA increased as the population size increased. In across-population prediction, no significant difference was observed in PA for oil and protein content among different models. The PA increased as the SNP number increased until a marker set consisted of 10,000 SNPs. This study provides reasonable suggestions and methods for breeders to utilize public datasets for GS. It will aid breeders in developing GS-assisted breeding strategies to develop elite soybean cultivars with high oil and protein contents.