bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Humanized Scg3-Neutralizing Antibodies for Anti-Angiogenic Therapy of Diabetic Retinopathy
Authors: Huang Chengchi , Waduge Prabuddha , Kaur Avinash , Tian Hong , Weng Christina Y. , Stout John Timothy , Pang Iok-Hou , Webster Keith A. , Li Wei
Published: September 2024

Secretogranin III (Scg3) is a diabetic retinopathy (DR)-restricted angiogenic factor identified in preclinical studies as a target for DR therapy. Previously, our group generated and characterized ML49.3, an anti-Scg3 monoclonal antibody (mAb) which we then converted into an EBP2 humanized antibody Fab fragment (hFab) with potential for clinical application. We also generated anti-Scg3 mT4 mAb and related EBP3 hFab. In this study, to identify the preferred hFab for DR therapy, we compared all four antibodies for binding, neutralizing and therapeutic activities in vitro and in vivo. Octet binding kinetics analyses revealed that ML49.3 mAb, EBP2 hFab, mT4 mAb and EBP3 hFab have Scg3-binding affinities of 35, 8.7, 0.859 and 0.116 nM, respectively. Both anti-Scg3 EBP2 and EBP3 hFabs significantly inhibited Scg3-induced proliferation and migration of human umbilical vein endothelial cells in vitro, and alleviated DR vascular leakage and choroidal neovascularization with high efficacy. Paired assays in DR mice revealed that intravitreally injected EBP3 hFab is 26.4% and 10.3% more effective than EBP2 hFab and aflibercept, respectively, for ameliorating DR leakage. In conclusion, this study confirms the markedly improved binding affinities of hFabs compared to mAbs and further identifies EBP3 hFab as the preferred antibody to develop for anti-Scg3 therapy.
Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae)
Authors: Johny Jibin , Große-Wilde Ewald , Kalinová Blanka , Roy Amit
Published: September 2024

The northern bark beetle, Ips duplicatus, is an emerging economic pest, reportedly infesting various species of spruce (Picea spp.), pine (Pinus spp.), and larch (Larix spp.) in Central Europe. Recent climate changes and inconsistent forest management practices have led to the rapid spread of this species, leaving the current monitoring strategies inefficient. As understanding the molecular components of pheromone detection is key to developing novel control strategies, we generated antennal transcriptomes from males and females of this species and annotated the chemosensory proteins. We identified putative candidates for 69 odorant receptors (ORs), 50 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 27 odorant-binding proteins (OBPs), including a tetramer-OBP, 9 chemosensory proteins (CSPs), and 6 sensory neuron membrane proteins (SNMPs). However, no sex-specific chemosensory genes were detected. The phylogenetic analysis revealed conserved orthology in bark beetle chemosensory proteins, especially with a major forest pest and co-habitant, Ips typographus. Recent large-scale functional studies in I. typographus chemoreceptors add greater significance to the orthologous sequences reported here. Nevertheless, identifying chemosensory genes in I. duplicatus is valuable to understanding the chemosensory system and its evolution in bark beetles (Coleoptera) and, generally, insects.
Development and Optimization of a Bromothymol Blue-Based PLA2 Assay Involving POPC-Based Self-Assemblies
Authors: Khan Shibbir Ahmed , Ilies Marc A.
Published: September 2024

Phospholipase A2 (PLA2) is a superfamily of phospholipase enzymes that dock at the water/oil interface of phospholipid assemblies, hydrolyzing the ester bond at the sn-2 position. The enzymatic activity of these enzymes differs based on the nature of the substrate, its supramolecular assemblies (micelle, liposomes), and their composition, reflecting the interfacial nature of the PLA2s and requiring assays able to directly quantify this interaction of the enzyme(s) with these supramolecular assemblies. We developed and optimized a simple, universal assay method employing the pH-sensitive indicator dye bromothymol blue (BTB), in which different POPC (3-palmitoyl-2-oleoyl-sn-glycero-1-phosphocholine) self-assemblies (liposomes or mixed micelles with Triton X-100 at different molar ratios) were used to assess the enzymatic activity. We used this assay to perform a comparative analysis of PLA2 kinetics on these supramolecular assemblies and to determine the kinetic parameters of PLA2 isozymes IB and IIA for each supramolecular POPC assembly. This assay is suitable for assessing the inhibition of PLA2s with great accuracy using UV-VIS spectrophotometry, being thus amenable for screening of PLA2 enzymes and their substrates and inhibitors in conditions very similar to physiologic ones.
Phenotypic, Metabolic and Genetic Adaptations of the Ficus Species to Abiotic Stress Response: A Comprehensive Review
Authors: Yuan Shengyun , Yin Tianxiang , He Hourong , Liu Xinyi , Long Xueyan , Dong Pan , Zhu Zhenglin
Published: September 2024

The Ficus genus, having radiated from the tropics and subtropics to the temperate zone worldwide, is the largest genus among woody plants, comprising over 800 species. Evolution of the Ficus species results in genetic diversity, global radiation and geographical differentiations, suggesting adaption to diverse environments and coping with stresses. Apart from familiar physiological changes, such as stomatal closure and alteration in plant hormone levels, the Ficus species exhibit a unique mechanism in response to abiotic stress, such as regulation of leaf temperature and retention of drought memory. The stress-resistance genes harbored by Ficus result in effective responses to abiotic stress. Understanding the stress-resistance mechanisms in Ficus provides insights into the genetic breeding toward stress-tolerant crop cultivars. Following upon these issues, we comprehensively reviewed recent progress concerning the Ficus genes and relevant mechanisms that play important roles in the abiotic stress responses. These highlight prospectively important application potentials of the stress-resistance genes in Ficus.
Multi-Omics Reveals the Role of Arachidonic Acid Metabolism in the Gut–Follicle Axis for the Antral Follicular Development of Holstein Cows
Authors: Guo Yajun , Wang Shiwei , Wu Xuan , Zhao Rong , Chang Siyu , Ma Chen , Song Shuang , Zeng Shenming
Published: September 2024

In vitro embryonic technology is crucial for improving farm animal reproduction but is hampered by the poor quality of oocytes and insufficient development potential. This study investigated the relationships among changes in the gut microbiota and metabolism, serum features, and the follicular fluid metabolome atlas. Correlation network maps were constructed to reveal how the metabolites affect follicular development by regulating gene expression in granulosa cells. The superovulation synchronization results showed that the number of follicle diameters from 4 to 8 mm, qualified oocyte number, cleavage, and blastocyst rates were improved in the dairy heifers (DH) compared with the non-lactating multiparous dairy cows (NDC) groups. The gut microbiota was decreased in Rikenellaceae_RC9_gut_group, Alistipes, and Bifidobacterium, but increased in Firmicutes, Cyanobacteria, Fibrobacterota, Desulfobacterota, and Verrucomicrobiota in the NDC group, which was highly associated with phospholipid-related metabolites of gut microbiota and serum. Metabolomic profiling of the gut microbiota, serum, and follicular fluid further demonstrated that the co-metabolites were phosphocholine and linoleic acid. Moreover, the expression of genes related to arachidonic acid metabolism in granulosa cells was significantly correlated with phosphocholine and linoleic acid. The results in granulosa cells showed that the levels of PLCB1 and COX2, participating in arachidonic acid metabolism, were increased in the DH group, which improved the concentrations of PGD2 and PGF2α in the follicular fluid. Finally, the expression levels of apoptosis-related proteins, cytokines, and steroidogenesis-related genes in granulosa cells and the concentrations of steroid hormones in follicular fluid were determinants of follicular development. According to our results, gut microbiota-related phosphocholine and linoleic acid participate in arachidonic acid metabolism in granulosa cells through the gut–follicle axis, which regulates follicular development. These findings hold promise for enhancing follicular development and optimizing oocyte quality in subfertile dairy cows.
Novel Role of the ALPI Gene Associated with Constipation Caused by Complement Component 3 Deficiency
Authors: Song Hee Jin , Kim Ji Eun , Roh Yu Jeong , Seol Ayun , Kim Tae Ryeol , Park Ki Ho , Park Eun Seo , Hong Jin Tae , Choi Sun Il , Hwang Dae Youn
Published: September 2024

Complement component 3 (C3) deficiency has recently been reported as one of the novel causes of constipation. To identify a unique gene specific to constipation caused by C3 deficiency, the total RNA extracted from the mid colon of C3 knockout (C3 KO) mice was hybridized to oligonucleotide microarrays, and the function of the candidate gene was verified in in vitro and in vivo models. C3 KO mice used for microarrays showed definite phenotypes of constipation. Overall, compared to the wild type (WT), 1237 genes were upregulated, and 1292 genes were downregulated in the C3 KO mice. Of these, the major genes included were lysine (K)-specific demethylase 5D (KDM5D), olfactory receptor 870 (Olfr870), pancreatic lipase (PNLIP), and alkaline phosphatase intestinal (ALPI). Specifically, the ALPI gene was selected as a novel gene candidate based on alterations during loperamide (Lop)-induced constipation and intestinal bowel disease (IBD). The upregulation of ALPI expression treated with acetate recovered the expression level of mucin-related genes in primary epithelial cells of C3 KO mice as well as most phenotypes of constipation in C3 KO mice. These results indicate that ALPI plays an important role as the novel gene associated with C3 deficiency-induced constipation.
Moonlighting Crypto-Enzymes and Domains as Ancient and Versatile Signaling Devices
Authors: Turek Ilona , Wong Aloysius , Domingo Guido , Vannini Candida , Bracale Marcella , Irving Helen , Gehring Chris
Published: September 2024

Increasing numbers of reports have revealed novel catalytically active cryptic guanylate cyclases (GCs) and adenylate cyclases (ACs) operating within complex proteins in prokaryotes and eukaryotes. Here we review the structural and functional aspects of some of these cyclases and provide examples that illustrate their roles in the regulation of the intramolecular functions of complex proteins, such as the phytosulfokine receptor (PSKR), and reassess their contribution to signal generation and tuning. Another multidomain protein, Arabidopsis thaliana K+ uptake permease (AtKUP5), also harbors multiple catalytically active sites including an N-terminal AC and C-terminal phosphodiesterase (PDE) with an abscisic acid-binding site. We argue that this architecture may enable the fine-tuning and/or sensing of K+ flux and integrate hormone responses to cAMP homeostasis. We also discuss how searches with motifs based on conserved amino acids in catalytic centers led to the discovery of GCs and ACs and propose how this approach can be applied to discover hitherto masked active sites in bacterial, fungal, and animal proteomes. Finally, we show that motif searches are a promising approach to discover ancient biological functions such as hormone or gas binding.
Maternal Plasma miRNAs as Early Biomarkers of Moderate-to-Late-Preterm Birth
Authors: Ramzan Farha , Rong Jing , Roberts Claire T. , O’Sullivan Justin M. , Perry Jo K. , Taylor Rennae , McCowan Lesley , Vickers Mark H.
Published: September 2024

Globally, preterm birth (PTB) is a primary cause of mortality and morbidity in infants, with PTB rates increasing worldwide over the last two decades. Biomarkers for accurate early prediction of PTB before the clinical event do not currently exist. Given their roles in the development and progression of many disease states, there has been increasing interest in the utility of microRNAs (miRNAs) as early biomarkers for pregnancy-related disorders, including PTB. The present study was designed to examine potential differences in miRNA abundances in maternal plasma from mothers with infants born following a moderate to late (28–36 weeks’ gestation, n = 54) spontaneous PTB (SPTB) compared to mothers with matched term infants (n = 54). Maternal plasma collected at 15 weeks’ gestation were utilised from the Auckland and Adelaide cohorts from the Screening for Pregnancy Endpoints (SCOPE) study. miRNAs in plasma were quantified using the NanoString nCounter expression panel (800 miRNAs). The top four most abundant miRNAs were significantly decreased in the plasma of mothers in the SPTB group with results consistent across both cohorts and pathway analysis was undertaken to examine the biological processes linked to the dysregulated miRNAs. The top candidate miRNAs (miRs-451a, −223-3p, let-7a-5p, and -126-3p) were linked to gene pathways associated with inflammation, apoptosis, and mitochondrial biogenesis. Moreover, miRNAs were consistently less abundant in the plasma of mothers of preterm infants across both sites, suggesting potential global dysregulation in miRNA biogenesis. This was supported by a significant downregulation in expression of key genes that are involved in miRNA biogenesis (DROSHA, DICER, and AGO2) across both sites in the SPTB group. In summary, the present study has identified miRNAs in maternal plasma that may provide predictive utility as early biomarkers for the risk of later SPTB. Importantly, these observations were conserved across two independent cohorts. Further, our data provide evidence for a persistent decrease in miRNA abundance in mothers who later experienced an SPTB, which is likely to have widespread consequences for gene regulation and epigenetic processes.
Gastrodin, a Promising Natural Small Molecule for the Treatment of Central Nervous System Disorders, and Its Recent Progress in Synthesis, Pharmacology and Pharmacokinetics
Authors: Dai Yanan , Ban Weikang , Yang Zhihong
Published: September 2024

Gastrodia elata Blume is a traditional medicinal and food homology substance that has been used for thousands of years, is mainly distributed in China and other Asian countries, and has always been distinguished as a superior class of herbs. Gastrodin is the main active ingredient of G. elata Blume and has attracted increasing attention because of its extensive pharmacological activities. In addition to extraction and isolation from the original plant, gastrodin can also be obtained via chemical synthesis and biosynthesis. Gastrodin has significant pharmacological effects on the central nervous system, such as sedation and improvement of sleep. It can also improve epilepsy, neurodegenerative diseases, emotional disorders and cognitive impairment to a certain extent. Gastrodin is rapidly absorbed and widely distributed in the body and can also penetrate the blood–brain barrier. In brief, gastrodin is a promising natural small molecule with significant potential in the treatment of brain diseases. In this review, we summarised studies on the synthesis, pharmacological effects and pharmacokinetic characteristics of gastrodin, with emphasis on its effects on central nervous system disorders and the possible mechanisms, in order to find potential therapeutic applications and provide favourable information for the research and development of gastodin.
Ginsenoside Rh2 Alleviates LPS-Induced Inflammatory Responses by Binding to TLR4/MD-2 and Blocking TLR4 Dimerization
Authors: Pan Shujuan , Peng Luyuan , Yi Qion , Qi Weijin , Yang Hui , Wang Hongying , Wang Lu
Published: September 2024

Lipopolysaccharide (LPS) triggers a severe systemic inflammatory reaction in mammals, with the dimerization of TLR4/MD-2 upon LPS stimulation serving as the pivotal mechanism in the transmission of inflammatory signals. Ginsenoside Rh2 (G-Rh2), one of the active constituents of red ginseng, exerts potent anti-inflammatory activity. However, whether G-Rh2 can block the TLR4 dimerization to exert anti-inflammatory effects remains unclear. Here, we first investigated the non-cytotoxic concentration of G-Rh2 on RAW 264.7 cells, and detected the releases of pro-inflammatory cytokines in LPS-treated RAW 264.7 cells, and then uncovered the mechanisms involved in the anti-inflammatory activity of G-Rh2 through flow cytometry, fluorescent membrane localization, Western blotting, co-immunoprecipitation (Co-IP), molecular docking and surface plasmon resonance (SPR) analysis in LPS-stimulated macrophages. Our results show that G-Rh2 stimulation markedly inhibited the secretion of LPS-induced interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and nitric oxide (NO). Additionally, G-Rh2 blocked the binding of LPS with the membrane of RAW 264.7 cells through direct interaction with TLR4 and MD-2 proteins, leading to the disruption of the dimerization of TLR4 and MD-2, followed by suppression of the TLR4/NF-κB signaling pathway. Our results suggest that G-Rh2 acts as a new inhibitor of TLR4 dimerization and may serve as a promising therapeutic agent against inflammation.
Exceptional Evolution of a Squamous Odontogenic Tumor in the Jaw: Molecular Approach
Authors: Alonso-Juarranz Miguel , Sen Oscar De La , Pérez Pablo , González-Corchón Maria Aranzazu , Cabezas-Camarero Santiago , Saiz-Pardo Melchor , Viñas-Lopez Jesus , Recio-Poveda Lucia , Botella Luisa María , Falahat Farzin
Published: September 2024

A squamous odontogenic tumor (SOT) is an epithelial locally benign neoplasia derived from the periodontium of the jaws. It is considered a lesion of low incidence. Predominantly, it affects the mandible, although both jaw bones may be involved. Here, we discuss the malignant clinical evolution of an SOT lesion in an 80-year-old female patient. The patient exhibited an expansive triangular lesion at the inferior right quadrant. Surgery was performed and an SOT was diagnosed (2019). Two years after, the lesion grew, and the analysis of the biopsy revealed SOT malignization with pleomorphic atypical squamous cells, characteristics of a squamous cell carcinoma (2021). Massive DNA sequencing of formalin-fixed–paraffin-embedded specimens of the initial and relapsed tumors indicated pathogenic mutations in RET and POLE genes in both tumors, loss of ALK, and gain of CDKN1B and MAP2K in the relapse. In addition, the clinical, radiographic, and microscopic features of this neoplasm are discussed and compared with those already published. The case presented contributes to the better understanding of this SOT tumor entity and to indicates its malignant evolution, together with its biological behavior and its histologic, clinical, and radiographic features. Also, it aims to stress the importance of deeper genetic analyses in rare diseases to uncover mutations that help to select a personalized treatment.
Comparative Analysis of Comprehensive Genomic Profile in Thymomas and Recurrent Thymomas Reveals Potentially Actionable Mutations for Target Therapies
Authors: Lococo Filippo , De Paolis Elisa , Evangelista Jessica , Dell’Amore Andrea , Giannarelli Diana , Chiappetta Marco , Campanella Annalisa , Sassorossi Carolina , Cancellieri Alessandra , Calabrese Fiorella , Conca Alessandra , Vita Emanuele , Minucci Angelo , Bria Emilio , Castello Angelo , Urbani Andrea , Rea Federico , Margaritora Stefano , Scambia Giovanni
Published: September 2024

Molecular profiles of thymomas and recurrent thymomas are far from being defined. Herein, we report an analysis of a comprehensive genetic profile (CGP) in a highly selected cohort of recurrent thymomas. Among a cohort of 426 thymomas, the tissue was available in 23 recurrent tumors for matching the biomolecular results obtained from primary and relapse samples. A control group composed of non-recurrent thymoma patients was selected through a propensity score match analysis. CGP was performed using the NGS Tru-SightOncology assay to evaluate TMB, MSI, and molecular alterations in 523 genes. CGP does not differ when comparing initial tumor with tumor relapse. A significantly higher frequency of cell cycle control genes alterations (100.0% vs. 57.1%, p = 0.022) is detected in patients with early recurrence (<32 months) compared to late recurrent cases. The CGPs were similar in recurrent thymomas and non-recurrent thymomas. Finally, based on NGS results, an off-label treatment or clinical trial could be potentially proposed in >50% of cases (oncogenic Tier-IIC variants). In conclusion, CGPs do not substantially differ between initial tumor vs. tumor recurrence and recurrent thymomas vs. non-recurrent thymomas. Cell cycle control gene alterations are associated with an early recurrence after thymectomy. Multiple target therapies are potentially available by performing a comprehensive CGP, suggesting that a precision medicine approach on these patients could be further explored.
Validation of an Artificial Intelligence-Based Model for Early Childhood Caries Detection in Dental Photographs
Authors: Schwarzmaier Julia , Frenkel Elisabeth , Neumayr Julia , Ammar Nour , Kessler Andreas , Schwendicke Falk , Kühnisch Jan , Dujic Helena
Published: September 2024

Background/Objectives: Early childhood caries (ECC) is a widespread and severe oral health problem that potentially affects the general health of children. Visual–tactile examination remains the diagnostic method of choice to diagnose ECC, although visual examination could be automated by artificial intelligence (AI) tools in the future. The aim of this study was the external validation of a recently published and freely accessible AI-based model for detecting ECC and classifying carious lesions in dental photographs. Methods: A total of 143 anonymised photographs of anterior deciduous teeth (ECC = 107, controls = 36) were visually evaluated by the dental study group (reference test) and analysed using the AI-based model (test method). Diagnostic performance was determined statistically. Results: ECC detection accuracy was 97.2%. Diagnostic performance varied between carious lesion classes (noncavitated lesions, greyish translucency/microcavity, cavitation, destructed tooth), with accuracies ranging from 88.9% to 98.1%, sensitivities ranging from 68.8% to 98.5% and specificities ranging from 86.1% to 99.4%. The area under the curve ranged from 0.834 to 0.964. Conclusions: The performance of the AI-based model is similar to that reported for the internal dataset used by developers. Further studies with independent image samples are required to comprehensively gauge the performance of the model.
The Intersection between Frailty, Diabetes, and Hypertension: The Critical Role of Community Geriatricians and Pharmacists in Deprescribing
Authors: Dinarvand Daniel , Panthakey Johann , Heidari Amirmohammad , Hassan Ahmed , Ahmed Mohamed H.
Published: September 2024

Background: Frailty is a clinical syndrome prevalent among the elderly, characterised by a decline in physiological reserves and increased susceptibility to stressors, resulting in higher morbidity and mortality. Diabetes and hypertension are common in frail older individuals, often leading to polypharmacy. In this narrative review, we aimed to evaluate the relationship between frailty, diabetes, and hypertension and to identify effective management strategies and future research directions. Methods: This narrative review was conducted using the Scopus, Medline, PubMed, Cochrane Library, and Google Scholar databases. Results: Frailty significantly impacts the management and prognosis of diabetes and hypertension, which, in turn, affects the progression of frailty. Managing these conditions often involves multiple drugs to achieve strict glycaemic control and blood pressure targets, leading to polypharmacy and associated morbidities, including orthostatic hypotension, falls, fractures, hypoglycaemia, and reduced medication adherence. Identifying frailty and implementing strategies like deprescribing can mitigate the adverse effects of polypharmacy and improve outcomes and quality of life. Despite the availability of effective tools for identifying frailty, many frail individuals continue to be exposed to complex treatment regimens for diabetes and hypertension, leading to increased hospital admissions, morbidity, and mortality. Conclusions: Managing diabetes and hypertension in the frail ageing population requires a multidisciplinary approach involving hospital and community geriatricians and pharmacists. This is important due to the lack of sufficient clinical trials dedicated to diabetes and hypertension in the context of frailty. Future large population studies are needed to assess the best approaches for managing diabetes and hypertension in frail individuals.
Land Regulation and Local Service Provision: Can Economic Growth and Environmental Protection Be Achieved Simultaneously?
Authors: Zhang Xiaodong , Duan Kaifeng , Yang Lun , Wei Xiaokun
Published: September 2024

This study examines the impact of China’s industrial land approval reform (ILAR) on the provision of subnational services. Utilizing the 2011 pilot reform policy approved by the State Council, we utilize a staggered Difference-in-Differences (DID) method to determine the reform’s impact on local economic growth and environmental protection. The findings reveal that the reform enhances local economic development and decreases pollution levels. Additionally, the reform significantly decreases land resource misallocation in pilot cities, enhancing productivity. We also find that local governments allocate more land to the tertiary sector through this reform, achieving industrial upgrading. The optimization effects are more pronounced in cities with higher fiscal pressure. Based on these findings, we recommend that policymakers sustain decentralization efforts and consider further incentives for cities under fiscal stress. We fill a gap in the literature by linking land use regulation with subnational service provision, contributing to the understanding of the socioeconomic benefits of decentralization and local government service levels.
Premature Damage in Bearing Steel in Relation with Residual Stresses and Hydrogen Trapping
Authors: Baur Maximilian , Khader Iyas , Kürten Dominik , Schieß Thomas , Kailer Andreas , Dienwiebel Martin
Published: September 2024

In this study, premature damage in cylindrical roller bearings made of 100Cr6 (SAE 52100) was investigated. For this purpose, full bearing tests were carried out using two different lubricant formulations with similar viscosities. Published research has pointed out the occurrence of tribo-chemical reactions that cause lubricant degradation and the release of hydrogen in tribo-contact. Hydrogen content measurements were conducted on tested samples, and these measurements showed dependence on the lubricant formulations. Hydrogen diffusion and trapping were identified as significant factors influencing premature damage. The measurement of trapping energies was conducted by thermal desorption spectroscopy, whereas residual stresses, which influence hydrogen diffusion and accumulation, were measured using X-ray diffraction. The measured trapping energies indicated that rolling contact caused the creation and release of hydrogen traps. Over-rolling resulted in changes in residual stress profiles in the materials, demonstrated by changes in stress gradients. These can be directly linked to subsurface hydrogen accumulation. Hence, it was possible to determine that the location of the microstructural damage (WEC) was correlated with the residual stress profiles and the subsurface von Mises stress peaks.
Simulation Model of a Steelmaking–Continuous Casting Process Based on Dynamic-Operation Rules
Authors: Shao Xin , Liu Qing , Chen Hongzhi , Zhang Jiangshan , Gao Shan , Li Shaoshuai
Published: September 2024

The steelmaking–continuous casting process (SCCP) is a complex manufacturing process which exhibits the distinct features of process manufacturing. The SCCP involves a variety of production elements, such as multiple process routes, a wide array of smelting and auxiliary devices, and a variety of raw and auxiliary materials. The production-simulation of SCCP holds a natural advantage in being able to accurately depict the intricate production behavior involved, and this serves as a crucial tool for optimizing the production operation of the SCCP. This paper thoroughly considers the various production elements involved in the SCCP, such as the fluctuation of the converter smelting cycle, fluctuation of heat weight, and ladle operation. Based on the Plant Simulation software platform, a dynamic simulation model of the SCCP is established and detailed descriptions are provided regarding the design of an SCCP using dynamic-operation rules. Additionally, a dynamic operational control program for the SCCP is developed using the SimTalk language, one which ensures the continuous operation of the caster in the SCCP, using the discrete simulation platform. The effectiveness of the proposed dynamic simulation model is verified by the total completion time of the production plan, the transfer time of the heat among the different processes, and the frequency of ladle turnover. The simulation’s results indicate that the dynamic simulation model has a satisfactory effect in simulating the actual production process. On this basis, the application effects of different schedules are compared and analyzed. Compared with a heuristic schedule, the optimized schedule based on the “furnace–machine coordinating” mode reduces the weighted value of total completion time by 8.7 min, reduces the weighted value of transfer waiting time by 45.5 min, and the number of rescheduling times is also reduced, demonstrating a better application effect and verifying the optimizing effect of the “furnace–machine coordinating” mode on the schedule.
Life-Cycle Assessment and Environmental Costs of Cement-Based Materials Manufactured with Mixed Recycled Aggregate and Biomass Ash
Authors: Agrela Francisco , Rosales Manuel , Alonso Mónica López , Ordóñez Javier , Cuenca-Moyano Gloria M.
Published: September 2024

The development of new building elements, such as concrete and mortar with sustainable materials, which produce a lower carbon footprint, is an achievable milestone in the short term. The need to reduce the environmental impact of the production of cement-based materials is of vital importance. This work focuses on the evaluation of the life-cycle assessment, production costs, mechanical performance, and durability of three mortars and three concrete mixtures in which mixed recycled aggregates (MRAs) and biomass bottom ash from olive waste (oBBA) were included to replace cement and aggregates. Powdered MRA and oBBA were also applied as complementary cementitious materials with a reduced environmental footprint. Chemical and physical tests were performed on the materials, and mechanical performance properties, life-cycle assessment, and life-cycle cost analysis were applied to demonstrate the technical and environmental benefits of using these materials in mortar and concrete mixtures. This research showed that the application of MRA and oBBA produced a small reduction in mechanical strength but a significant benefit in terms of life-cycle population and environmental costs. The results demonstrated that finding long-term mechanical strength decreases between 2.7% and 14% for mortar mixes and between 1.7% and 10.4% for concrete mixes. Although there were small reductions in mechanical performance, the savings in environmental and monetary terms make the feasibility of manufacturing these cement-based materials feasible and interesting for both society and the business world. CO2 emissions are reduced by 25% for mortar mixes and 12% for concrete mixes with recycled materials, and it is possible to reduce the cost per cubic meter of mortar production by 20%, and the savings in the cost of production of a cubic meter of concrete is 13.8%.
Characteristics of Circulating Fluidized Bed Combustion (CFBC) Ash as Carbon Dioxide Storage Medium and Development of Construction Materials by Recycling Carbonated Ash
Authors: Wie Young Min , Lee Ki Gang , Lee Kang Hoon
Published: September 2024

This study validates the attributes of the mineral carbonation process employing circulating fluidized bed combustion (CFBC) ash, which is generated from thermal power plants, as a medium for carbon storage. Furthermore, an examination was conducted on the properties of construction materials produced through the recycling of carbonated circulating fluidized bed combustion (CFBC) ash. The carbonation characteristics of circulating fluidized bed combustion (CFBC) ash were investigated by analyzing the impact of CO2 flow rate and solid content. Experiments were conducted to investigate the use of it as a concrete admixture by replacing cement at varying percentages ranging from 0% to 20% by weight. The stability and setting time were subsequently measured. To produce foam concrete, specimens were fabricated by substituting 0 to 30 wt% of the cement. Characteristics of the unhardened slurry, such as density, flow, and settlement depth, were measured, while characteristics after hardening, including density, compressive strength, and thermal conductivity, were also assessed. The findings of our research study validated that the carbonation rate of CFBC ash in the slurry exhibited distinct characteristics compared to the reaction in the solid–gas system. Manufactured carbonated circulating fluidized bed combustion (CFBC) ash, when used as a recycled concrete mixture, improved the initial strength of cement mortar by 5 to 12% based on the 7-day strength. In addition, it replaced 25 wt% of cement in the production of foam concrete, showing a density of 0.58 g/cm3, and the 28-day strength was 2.1 MPa, meeting the density standard of 0.6 grade foam concrete.
Preparation of Metallized Pellets for Steelmaking by Hydrogen Cooling Reduction with Different Cooling Rates
Authors: Luo Guanwen , Peng Zhiwei , Gao Kangle , Fan Wanlong , Tian Ran , Yi Lingyun , Rao Mingjun
Published: September 2024

To utilize the sensible heat of hot roasted iron ore pellets with no CO2 emission in the production of metallized pellets for direct steelmaking, the pellets were reduced in H2 during their cooling process with variable cooling rates. When the cooling rate decreased from 5.2 °C/min to 2.0 °C/min, the total iron content, reduction degree, and iron metallization degree of the pellets increased continuously from 74.0 wt%, 52%, and 31.1% to 84.9 wt%, 93.4%, and 89.2%, respectively. However, the compressive strength of the pellets increased initially from 2100 N/p to 2436 N/p and then decreased considerably to 841 N/p. As the cooling rate decreased, more Fe2O3 was reduced to Fe with diminishing FeO and Fe2SiO4. The porosity of the pellets increased from 23.9% to 54.3%, with higher distribution uniformity of pores. The morphology of metallic iron particles also transited from a layered form to a spherical form and lastly to a porous reticular form. Meanwhile, the metallic iron particles in the pellets grew evidently with more uniform distributions. When the cooling rate was 3.7 °C/min, the resulting metallized pellets had the reduction degree of 74.2%, iron metallization degree of 66.9%, and the highest compressive strength of 2436 N/p, in association with the spherical morphology and relatively large size of metallic iron particles.
Use of a Glaciogene Marine Clay (Ilulissat, Greenland) in a Pilot Production of Red Bricks
Authors: Belmonte Louise J. , Ottosen Lisbeth M. , Kirkelund Gunvor M.
Published: September 2024

Uplifted occurrences of fine-grained glaciogene marine sediments are found throughout the northern hemisphere. These sediments could be used to produce local construction materials, to rely less on imported construction materials from southern regions. In this study, a representative occurrence from Ilulissat, West Greenland, was investigated as a potential resource for local brick production. The study comprised three parts: (1) raw material characterization based on grain size distribution, major element chemistry, including total carbon, sulfur, and chloride concentrations, mineralogy, morphology, and Atterberg limits; (2) the production of test bricks at a Danish brickwork; and (3) testing of the bricks based on total shrinkage, water absorption, hygroscopic adsorption, open porosity, bulk density, compression strength, and mineralogy. The bricks produced proved to have excellent compression strength, low open porosity, and low water absorption. The shrinkage could be reduced by adding 10% chamotte to the marine sediment. Based on the investigated properties, this indicates that this type of clay is highly suitable as a resource for bricks.
Influence of Ag Doping on Wide-Emperature Tribological Properties of γ-Fe2O3@SiO2 Nanocomposite Coatings on Steel
Authors: Zeng Qunfeng , Sun Shichuan , Jia Qian
Published: September 2024

γ-Fe2O3@SiO2-Ag nanocomposite coatings were prepared to investigate the lubrication performances of the nanocomposite coatings under a wide range of temperatures. The effect of Ag doping on the tribological properties of γ-Fe2O3@SiO2-Ag nanocomposite coatings was studied from room temperature to 600 °C, and the synergistic effect of Ag and oxides in the nanocomposite coatings was investigated. The coefficient of friction and the wear rate of γ-Fe2O3@SiO2-Ag nanocomposite coatings decrease with an increase in Ag content. The tribological properties of 24 wt.%Ag of the nanocomposite coatings are excellent. The stable coefficient of friction is 0.25 at 100 °C and the coefficient of friction is reduced to 0.05 at 500 °C. It was found that the synergistic effect of γ-Fe2O3 and Ag is helpful in improving the tribological properties of γ-Fe2O3@SiO2-Ag nanocomposite coatings over a wide temperature range. Ag plays a lubricating role at low and medium temperatures and oxides play a role in lubrication at high temperatures.
First Principles Calculation of the Effect of Cu Doping on the Mechanical and Thermodynamic Properties of Au-2.0Ni Solder
Authors: Wei Yan , Dai Hua , Chen Li , Wang Xian , Cai Hongzhong , Zhang Jiankang , Xu Ying , Wang Xingqiang , Guo Junmei , Yuan Zhentao , Wang Xiao
Published: September 2024

To meet the demands for high-temperature performance and lightweight materials in aerospace engineering, the Au-Ni solder is often utilized for joining dissimilar materials, such as Ti3Al-based alloys and Ni-based high-temperature alloys. However, the interaction between Ti and Ni can lead to the formation of brittle phases, like Ti2Ni, TiNi, and TiNi3, which diminish the mechanical properties of the joint and increase the risk of crack formation during the welding process. Cu doping has been shown to enhance the mechanical properties and high-temperature stability of the Au-Ni brazed joint’s central area. Due to the difficulty in accurately controlling the solid solution content of Cu in the Au-Ni alloy, along with the high cost of Au, traditional experimental trial-and-error methods are insufficient for the development of Au-based solders. In this study, first principles calculations based on density functional theory were employed to analyze the effect of Cu content on the stability of the Au-2.0Ni-xCu (x = 0, 0.25, 0.5, 0.75, 1.0, 1.25 wt%) alloy phase structure. The thermal properties of the alloy were determined using Gibbs software fitting. The results indicate that the Au-2.0Ni-0.25Cu alloy exhibits the highest plastic toughness (B/G = 5.601, ν = 0.416, Cauchy pressure = 73.676 GPa) and a hardness of 1.17 GPa, which is 80% higher than that of Au-2.0Ni. This alloy balances excellent strength and plastic toughness, meeting the mechanical performance requirements of brazed joints. The constant pressure specific heat capacity (Cp) of the Au-2.0Ni-xCu alloy is higher than that of Au-2.0Ni and increases with Cu content. At 1000 K, the Cp of the Au-2.0Ni-0.25Cu alloy is 35.606 J·mol−1·K−1, which is 5.88% higher than that of Au-2.0Ni. The higher Cp contributes to enhanced high-temperature stability. Moreover, the linear expansion coefficient (CTE) of the Au-2.0Ni-0.25Cu alloy at 1000 K is 8.76 × 10−5·K−1, only 0.68% higher than Au-2.0Ni. The lower CTE helps to reduce the risk of solder damage caused by thermal stress. Therefore, the Au-2.0Ni-0.25Cu alloy is more suitable for brazing applications in high-temperature environments due to its excellent mechanical properties and thermal stability. This study provides a theoretical basis for the performance optimization and engineering application of the Au-2.0Ni-xCu alloy as a gold-based solder.
A Comparative Study of Ni-Based Catalysts Prepared by Various Sol–Gel Routes
Authors: Al Khudhair Atheer , Bouchmella Karim , Andrei Radu Dorin , Hulea Vasile , Mehdi Ahmad
Published: September 2024

The use of heterogeneous catalysts to increase the development of green chemistry is a rapidly growing area of research to save industry money. In this paper, mesoporous SiO2-Al2O3 mixed oxide supports with various Si/Al ratios were prepared using two different sol–gel routes: hydrolytic sol–gel (HSG) and non-hydrolytic sol–gel (NHSG). The HSG route was investigated in both acidic and basic media, while the NHSG was explored in the presence of ethanol and diisopropyl ether as oxygen donors. The resulting SiO2-Al2O3 mixed oxide supports were characterized using EDX, N2 physisorption, powder XRD, 29Si, 27Al MAS-NMR and NH3-TPD. The mesoporous SiO2-Al2O3 supports prepared by NHSG seemed to be more regularly distributed and also more acidic. Consequently, a simple one-step NHSG (ether and alcohol routes) was selected to prepare mesoporous and acidic SiO2-Al2O3-NiO mixed oxide catalysts, which were then evaluated in ethylene oligomerization. The samples prepared by the NHSG ether route showed better activity than those prepared by the NHSG alcohol route in the oligomerization of ethylene at 150 °C.
Synthesis of Tumor Selective Indole and 8-Hydroxyquinoline Skeleton Containing Di-, or Triarylmethanes with Improved Cytotoxic Activity
Authors: Hegedűs Dóra , Szemerédi Nikoletta , Petrinca Krisztina , Berkecz Róbert , Spengler Gabriella , Szatmári István
Published: September 2024

The reaction between glycine-type aminonaphthol derivatives substituted with 2- or 1-naphthol and indole or 7-azaindole has been tested. Starting from 2-naphthol as a precursor, the reaction led to the formation of ring-closed products, while in the case of a 1-naphthol-type precursor, the desired biaryl ester was isolated. The synthesis of a bifunctional precursor starting from 5-chloro-8-hydroxyquinoline, morpholine, and ethyl glyoxylate via modified Mannich reaction is reported. The formed Mannich base 10 was subjected to give bioconjugates with indole and 7-azaindole. The effect of the aldehyde component and the amine part of the Mannich base on the synthetic pathway was also investigated. In favor of having a preliminary overview of the structure-activity relationships, the derivatives have been tested on cancer and normal cell lines. In the case of bioconjugate 16, as the most powerful scaffold in the series bearing indole and a 5-chloro-8-hydroxyquinoline skeleton, a potent toxic activity against the resistant Colo320 colon adenocarcinoma cell line was observed. Furthermore, this derivative was selective towards cancer cell lines showing no toxicity on non-tumor fibroblast cells.
Electrospun PVP Fibers as Carriers of Ca2+ Ions to Improve the Osteoinductivity of Titanium-Based Dental Implants
Authors: Roknić Janina , Despotović Ines , Katić Jozefina , Petrović Željka
Published: September 2024

Although titanium and its alloys are widely used as dental implants, they cannot induce the formation of new bone around the implant, which is a basis for the functional integrity and long-term stability of implants. This study focused on the functionalization of the titanium/titanium oxide surface as the gold standard for dental implants, with electrospun composite fibers consisting of polyvinylpyrrolidone and Ca2+ ions. Polymer fibers as carriers of Ca2+ ions should gradually dissolve, releasing Ca2+ ions into the environment of the implant when it is immersed in a model electrolyte of artificial saliva. Scanning electron microscopy, energy dispersive X-ray spectroscopy and attenuated total reflectance Fourier transform infrared spectroscopy confirmed the successful formation of a porous network of composite fibers on the titanium/titanium oxide surface. The mechanism of the formation of the composite fibers was investigated in detail by quantum chemical calculations at the density functional theory level based on the simulation of possible molecular interactions between Ca2+ ions, polymer fibers and titanium substrate. During the 7-day immersion of the functionalized titanium in artificial saliva, the processes on the titanium/titanium oxide/composite fibers/artificial saliva interface were monitored by electrochemical impedance spectroscopy. It can be concluded from all the results that the composite fibers formed on titanium have application potential for the development of osteoinductive and thus more biocompatible dental implants.
Monitoring the Quality of Life and the Relationship between Quality of Life, Dietary Intervention, and Dietary Adherence in Patients with Coeliac Disease
Authors: Dakó Eszter , Dakó Sarolta , Papp Veronika , Juhász Márk , Takács Johanna , Csobod Éva Csajbókné , Pálfi Erzsébet
Published: September 2024

Inadequate adherence to a gluten-free diet in coeliac disease triggers autoimmune reactions and can reduce the quality of life. The strict diet requires constant vigilance, which can cause psychological distress. Our research aimed to assess the quality of life in adult patients with coeliac disease and to find a correlation between quality of life, dietary intervention, and adherence. The study included 51 adult patients with coeliac disease who completed a quality-of-life questionnaire. Adherence was assessed using serological tests and a dietary adherence test. The patients were divided into two groups: those on a gluten-free diet for at least three months (Group I) and newly diagnosed patients (Group II). Group I showed a significant decrease in the dysphoria subscale of the quality-of-life test between the first and last surveys. Poor quality of life was associated with worse adherence in Group II. A higher “Health concerns” quality of life subscale score was also associated with worse adherence in Group II. Our results suggest that dietetic care may be beneficial for patients with coeliac disease by reducing dysphoria. We recommend regular and long-term dietary monitoring from diagnosis to ensure adherence to a gluten-free diet and to maintain quality of life.
Correction: Li et al. Selenomethionine Inhibited HADV-Induced Apoptosis Mediated by ROS through the JAK-STAT3 Signaling Pathway. Nutrients 2024, 16, 1966
Authors: Li Chuqing , Liu Xia , Li Jiali , Lai Jia , Su Jingyao , Zhu Bing , Gao Buyun , Li Yinghua , Zhao Mingqi
Published: September 2024

In the original publication [...]