bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1
Authors: Ravanfar Seyed Ali , Achor Diann S. , Killiny Nabil , Shilts Turksen , Chen Yuting , El-Mohtar Choaa , Stelinski Lukasz L. , Bonning Bryony C. , Orbović﻿﻿﻿ Vladimir
Published: May 2022

The curry leaf tree, Bergera koenigii, is highly attractive to the Asian citrus psyllid, Diaphorina citri, which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus production in Florida and in other citrus-producing countries. As D. citri exhibits high affinity for feeding on young leaves of B. koenigii, transgenic B. koenigii expressing bacteria-derived pesticidal proteins such as Cry1Ba1 have potential for D. citri management when planted in or adjacent to citrus groves. Importantly, the plant pathogenic bacterium that causes citrus greening does not replicate in B. koenigii. Transgenic plants of B. koenigii were produced by insertion of the gene encoding the active core of the pesticidal protein Cry1Ba1 derived from Bacillus thuringiensis. The transformation success rate was low relative to that of other citrus, at 0.89%. T-DNA integration into the genome and cry1ba1 transcription in transgenic plants were confirmed. Transgenic plants expressing Cry1Ba1 differed from wild-type plants, differed in photosynthesis parameters and hormone levels in some instances, and a marked delay in wilting of detached leaves. The gut epithelium of D. citri fed on transgenic plants was severely damaged, consistent with Cry1Ba1-mediated pore formation, confirming expression of the pesticidal protein by transgenic B. koenigii. These results demonstrate that transgenic B. koenigii expressing bacteria-derived pesticidal proteins can be produced for potential use as trap plants for suppression of D. citri populations toward protection of citrus groves from citrus greening.
Implications of COVID-19 Innovations for Social Interaction: Provisional Insights From a Qualitative Study of Ghanaian Christian Leaders
Authors: Adams Glenn , Osei-Tutu Annabella , Affram Adjeiwa Akosua , Phillips-Kumaga Lilian , Dzokoto Vivian Afi Abui
Published: May 2022

Responses to the COVID-19 pandemic prompted people and institutions to turn to online virtual environments for a wide variety of social gatherings. In this perspectives article, we draw upon our previous work and interviews with Ghanaian Christian leaders to consider implications of this shift. Specifically, we propose that the shift from physical to virtual interactions mimics and amplifies the neoliberal individualist experience of abstraction from place associated with Eurocentric modernity. On the positive side, the shift from physical to virtual environments liberates people to selectively pursue the most fulfilling interactions, free from constraints of physical distance. On the negative side, the move from physical to virtual space necessitates a shift from material care and tangible engagement with the local community to the psychologization of care and pursuit of emotional intimacy in relations of one’s choosing—a dynamic that further marginalizes people who are already on the margins. The disruptions of the pandemic provide an opportunity to re-set social relations, to design ways of being that better promote sustainable collective well-being rather than fleeting personal fulfillment.
Individual Dispositions and Situational Stressors in Competitive Sport: The Role of Stress Mindset in the Cognitive Appraisals Processes
Authors: Čopec Dajana , Karlović Vragolov Matea , Buško Vesna
Published: May 2022

Personality has widely been documented to play an important role in the cognitive appraisal and stress processes. Emerging studies highlight the stress mindset as a new concept that could add to the understanding of individual differences in stress experiences. This study aimed to examine the relative contribution of Big Five personality dimensions and stress mindset in accounting for measures of cognitive appraisals of stress among the competing athletes. The study was conducted on a sample of 125 collegiate athletes of both genders who actively compete in sport. All the participants were regular undergraduate or graduate students at the Faculty of Kinesiology of the University of Zagreb. A questionnaire including demographic information about athletes and their sport career, stress mindset measure (SMM), situation-specific cognitive appraisal scale, sources of stress scale, and personality scale measured by IPIP-50 was administered in an online form using the Google Forms platform. Multivariate hierarchical regression procedures resulted in somewhat different predictor structures accounting for cognitive appraisals of threat, loss, and challenge, used as criterion variables. The set of Big Five personality dimensions and stress mindset measure proved to have a significant additive contribution to the explanation of each of the three cognitive appraisal criterion variances. The study results support the current body of literature suggesting a unique role of the stress mindset construct in explaining individual differences in cognitive stress appraisal among athletes above and beyond general personality dimensions.
Effects of a Pair Programming Educational Robot-Based Approach on Students’ Interdisciplinary Learning of Computational Thinking and Language Learning
Authors: Hsu Ting-Chia , Chang Ching , Wu Long-Kai , Looi Chee-Kit
Published: May 2022

Using educational robots (ERs) to integrate computational thinking (CT) with cross-disciplinary content has gone beyond Science, Technology, Engineering, and Mathematics (STEM), to include foreign-language learning (FL) and further cross-context target-language (TL) acquisition. Such integration must not solely emphasise CT problem-solving skills. Rather, it must provide students with interactive learning to support their target-language (TL) interaction while reducing potential TL anxiety. This study aimed to validate the effects of the proposed method of pair programming (PP) along with question-and-response interaction in a board-game activity on young learners’ CT skills and TL learning across contexts. Two Grade 6 classes, one with 16 students who were studying Chinese as a Second Language (CSL) and the other with 16 students who were studying English as a Foreign Language (EFL), participated in the activity. A series of instruments on achievement assessment, questionnaires on CT skills and TL anxiety, and sequential learning behaviour analysis were used to critically examine the results. The main conclusion is that the EFL group showed better social skills of cooperation on CT and lower TL learning anxiety, while the CSL group demonstrated better problem-solving skills in CT, but presented more behaviours of trial-and-error loops. Results not only contribute suggestions for cross-disciplinary learning but also provide support for cross-context instruction beyond educational coursework.
Evolutionary Game Analysis of Construction Workers' Unsafe Behaviors Based on Incentive and Punishment Mechanisms
Authors: Zhu Jianbo , Zhang Ce , Wang Shuyi , Yuan Jingfeng , Li Qiming
Published: May 2022

Construction is one of the most dangerous industries because of its open working environment and risky construction conditions. In the process of construction, risk events cause great losses for owners and workers. Most of the risk events are closely related to unsafe behaviors of workers. Therefore, it is of great significance for contractors to establish management measures, e.g., incentive and punishment mechanism, to induce workers to reduce unsafe behaviors. This paper aims to take the incentive and punishment mechanism into consideration and develop an evolutionary game model to improve the effectiveness of safety management. The evolutionary stability strategies which can help reduce unsafe behaviors are obtained and analyzed. Results show that there are 12 equilibrium strategies under the condition of different parameters. Specifically, the incentive and punishment mechanism has played an important role for the evolution direction. A balanced incentive and punishment mechanism for the investment and positive stimulus for workers can effectively promote both sides to take positive behaviors, and then realize good evolutionary stable situations. In addition, the initial perceptions of both sides have a decisive impact on the evolution direction. Strengthening communication with the mutual trust between both sides can improve safety performance of both sides. This study is valuable for contractors to design appropriate incentive and punishment measures and establish relevant strategies to promote safe behaviors of construction workers.
Cognitive and Neuropsychiatric Features of COVID-19 Patients After Hospital Dismission: An Italian Sample
Authors: Cian Veronica , De Laurenzis Alessandro , Siri Chiara , Gusmeroli Anna , Canesi Margherita
Published: May 2022

Background and Aims

Recent studies suggest cognitive, emotional, and behavioral impairments occur in patients after SARS-CoV-2 infection. However, studies are limited to case reports or case series and, to our knowledge, few of them have control groups. This study aims to assess the prevalence of neuropsychological and neuropsychiatric impairment in patients after hospitalization.

Methods

We enrolled 29 COVID+ patients (M/F: 17/12; age 58.41 ± 10.00 years; education 11.07 ± 3.77 years, 2 left handers) who needed hospitalization but no IC, about 20 days post-dismission, and 29 COVID− healthy matched controls. Neuropsychological and neuropsychiatric assessments were conducted via teleneuropsychology using the following tests: MMSE, CPM47, RAVLT, CDT, Digit-Span Forward/Backward, Verbal fluencies; BDI-II, STAI. People with previous reported cognitive impairment and neurological or psychiatric conditions were excluded. Clinical and demographics were collected. Comparison between groups was conducted using parametric or non-parametric tests according to data distribution (T-test, Mann Withney-U test; Chi-square goodness of fit). Within COVID+ group, we also evaluated the correlation between the cognitive and behavioral assessment scores and clinical variables collected.

Results

Among COVID+, 62% had at least one pathological test (vs. 13% in COVID−; p = 0.000) and significantly worst performances than COVID− in RAVLT learning (42.55 ± 10.44 vs. 47.9 ± 8.29, p = 0.035), RAVLT recall (8.79 ± 3.13 vs. 10.38 ± 2.19, p = 0.03), and recognition (13.69 ± 1.47 vs. 14.52 ± 0.63, p = 0.07). STAI II was higher in COVID− (32.69 ± 7.66 vs. 39.14 ± 7.7, p = 0.002). Chi-square on dichotomous values (normal/pathological) showed a significant difference between groups in Digit backward test (pathological 7/29 COVID+ vs. 0/29 COVID−; p = 0.005).

Conclusions

Patients COVID+ assessed by teleneuropsychology showed a vulnerability in some memory and executive functions (working memory, learning, delayed recall, and recognition). Intriguingly, anxiety was higher in the control group. Our findings therefore confirm the impact of COVID-19 on cognition even in patients who did not need IC. Follow-up is needed to evaluate the evolution of COVID-19-related cognitive deficit.

Clinical Trial Registration

[ClinicalTrials.gov], identifier [NCT05143320].
Glutamic Acid Decarboxylase 1 Gene Methylation and Panic Disorder Severity: Making the Connection by Brain Gray Matter Volume
Authors: Wu Huiqin , Zhong Yuan , Xu Huazhen , Ding Huachen , Yuan Shiting , Wu Yun , Liu Gang , Liu Na , Wang Chun
Published: May 2022

Objective

This study aimed to test the hypothesis that the relationship between glutamic acid decarboxylase (GAD) 1 gene methylation and severity of clinical symptoms of panic disorder (PD) is mediated by the effect of GAD1 gene methylation on gray matter volume (GMV) and the effect of GMV on PD.

Methods

Panic disorder (n = 24) patients were recruited consecutively from the Affiliated Brain Hospital of Nanjing Medical University through outpatient and public advertising, eligible healthy controls (HCs) (n = 22) were recruited from public advertising. We compared GMV and GAD1 gene methylation in PD and HCs to estimate the differences, and on the basis of the relationship between gray matter volumes and GAD1 gene methylation in PD patients was evaluated, the role of GMV as a mediator of GAD1 gene methylation and PD clinical symptoms was analyzed.

Results

Panic disorder patients had significantly lower methylation in the GAD1 promoter region on Cytosine-phosphate-guanine (CPG) 7 than HCs (t = 2.380, p = 0.021). Pearson correlation analysis found a significant negative association between cg171674146 (cg12) site and clinical severity (n = 24, r = −0.456, p = 0.025). Compared to HCs, patients with PD had decreased gray matter volumes in several brain regions, which were also associated with PD severity. Left postcentral gyrus (PoCG) GMV mediated the association between cg12 methylation and PD severity, and there was a significant mediation effect of right angular gyrus (ANG) gray matter volumes on the relationship between cg12 methylation and PD severity.

Limitation

No direct results can be derived for methylation patterns in different brain regions; the study is cross-sectional; relatively small size.
Efficiency of Inhaled Cannabidiol in Cannabis Use Disorder: The Pilot Study Cannavap
Authors: Cleirec Grégoire , Desmier Esther , Lacatus Cristina , Lesgourgues Simon , Braun Anais , Peloso Claire , Obadia Chanaëlle
Published: May 2022

Introduction

Cannabidiol (CBD), the second most prevalent cannabinoid found in cannabis, is considered to be safe for use. Studies suggest that CBD may be of benefit in treating cannabis use disorder (CUD). In clinical practice, CBD is already being used by patients who are trying to reduce or stop their cannabis consumption. The aim of this study was to assess the potential of CBD inhaled using a vaping device in CUD.

Methods

This was an exploratory, observational, non-randomized, open-label study conducted at an Addiction Support and Prevention Center in Paris. The primary endpoint was a reduction of at least 50% in the reported number of joints consumed daily at 12 weeks. The participants were given an electronic cigarette along with liquid containing CBD. Nicotine at 6 mg/ml could be added in case of co-consumption of tobacco. They were assessed once a week and the CBD liquid dose was adjusted based on withdrawal signs and cravings (33.3, 66.6 or 100 mg/mL).

Results

Between November 2020 and May 2021, 20 patients were included and 9 (45%) completed the follow-up. All of the participants used tobacco, and were provided a liquid with nicotine. At 12 weeks, 6 patients (30%) had reduced their daily cannabis consumption by at least 50%. The mean number of joints per day was 3, compared to 6.7 at baseline. The mean amount of CBD inhaled per day was 215.8 mg. No symptomatic treatment for cannabis withdrawal was prescribed. Mild adverse effects attributable to CBD and not requiring the prescription of any medicines were reported in a few patients.

Conclusion

This research provides evidence in favor of the use of CBD in CUD. It also highlights the benefits of inhalation as the route of CBD administration in patients who use cannabis: inhalation can allow users to self-titrate CBD based on their withdrawal symptoms and cravings. This study illustrates the interest of proposing an addictological intervention targeting at the same time tobacco and cannabis dependence in users who are co-consumers. A double-blind, randomized, placebo-controlled clinical trial is needed to assess the efficacy of inhaled CBD in CUD.

Study registration number (IDRCB) issued by the ANSM (Agence nationale de sécurité du médicament et des produits de santé—French National Agency for Medicines and Health Products Safety): 2018-A03256-49. This study received IEC approval from the CPP Sud-Ouest et Outre-Mer 1 (South-West and Overseas 1 IEC) on 15/06/2020 (CPP 1-19-041/ID 3012).
Using Digital Platform Approach to Reduce Salt Intake in a Sample of UAE Population: An Intervention Study
Authors: Jarrar Amjad H. , Al Dhaheri Ayesha S. , Lightowler Helen , Cheikh Ismail Leila , Al-Meqbaali Fatima , Bataineh Mo'ath F. , Alhefeiti Aseilah , Albreiki Maithah , Albadi Nouf , Alkaabi Salama , Thondre Pariyarath S.
Published: May 2022

Background

Non-communicable diseases (NCDs) are the leading causes of mortality globally, accounting for more deaths than all other causes combined. World Health Organization launched its initiative in 2013 to reduce the intake of salt, the number of countries that have national sodium reduction strategies reached to 89 countries in 2017. In 2020, a study conducted in UAE showed more than 65% of the population exceeded WHO recommendations for salt intake. This study aimed to measure effectiveness of using digital platform approach to deliver educational materials to facilitate salt reduction in a sample of UAE population.

Methods

A controlled parallel intervention study was conducted in 2020. A sample of 121 participants completed the study and fulfilled the inclusion criteria with female to male ratio of (0.95:1.05). Participants were distributed randomly into three groups Control group, WhatsApp group, and Electronic Brochures group. Educational materials were distributed among participants of WhatsApp and Electronic brochures groups for 6-weeks. 24-h urinary excretion for sodium, potassium and creatinine, were measured in addition to KAP questionnaire and physical activity on two occasions at baseline and endpoint after 10-weeks (6-weeks of educational intervention).

Results

Both intervention groups showed a reduction in sodium with 278 mg (p < 0.001) for WhatsApp group (n = 41) and 169 mg (p < 0.018) for Electronic brochures group (n = 41), while Control group didn't show any significant change. Moreover, the percentage of participants exceeding WHO recommendation of sodium intake was significantly reduced at the end of intervention, (p = 0.004). WhatsApp group was more efficient in the percentage of reduction of participants exceeding WHO recommendation compared with baseline, with p = 0.023. A significant reduction in the practice toward adding salt during cooking, use of table salt, adding salt before tasting the foods and use of chicken stocks for both intervention groups was noted with p < 0.05. Intervention groups showed a significant improvement (p < 0.001) in Food and Health related knowledge after 6-weeks of intervention.

Conclusion

The digital platform approach such as WhatsApp and Electronic Brochure were effective in salt reduction. This study proves that UAE population is ready to reduce salt intake with appropriate education materials and easy delivery approach.
A Compliance–Reactance Framework for Evaluating Human-Robot Interaction
Authors: Boos Annika , Herzog Olivia , Reinhardt Jakob , Bengler Klaus , Zimmermann Markus
Published: May 2022

When do we follow requests and recommendations and which ones do we choose not to comply with? This publication combines definitions of compliance and reactance as behaviours and as affective processes in one model for application to human-robot interaction. The framework comprises three steps: human perception, comprehension, and selection of an action following a cue given by a robot. The paper outlines the application of the model in different study settings such as controlled experiments that allow for the assessment of cognition as well as observational field studies that lack this possibility. Guidance for defining and measuring compliance and reactance is outlined and strategies for improving robot behaviour are derived for each step in the process model. Design recommendations for each step are condensed into three principles on information economy, adequacy, and transparency. In summary, we suggest that in order to maximise the probability of compliance with a cue and to avoid reactance, interaction designers should aim for a high probability of perception, a high probability of comprehension and prevent negative affect. Finally, an example application is presented that uses existing data from a laboratory experiment in combination with data collected in an online survey to outline how the model can be applied to evaluate a new technology or interaction strategy using the concepts of compliance and reactance as behaviours and affective constructs.
The Water-Energy-Food Nexus Beyond “Technical Quick Fix”: The Case of Hydro-Development in the Blue Nile Basin, Ethiopia
Authors: Müller-Mahn Detlef , Gebreyes Million , Allouche Jeremy , Debarry Annapia
Published: May 2022

This paper builds upon empirical material from a case study of two dam sites in Ethiopia to revisit nexus narratives from a political ecology perspective. The two dams on tributaries of the Upper Blue Nile are examples of the success of hydro-development in increasing food and energy production, but at the same time they are evidence of the controversial effects these developments have on local populations. The paper argues that conventional nexus thinking has often been too water- and economy-centric, and too much focussed on a “technical quick fix” instead of a holistic approach. The paper calls for a broadening of nexus perspectives in order to better acknowledge the social complexity of hydro-development in local contexts, to understand the political construction of scarcity, and to combine different knowledges at the science-practice interface.
Good or Bad? The Double Face of Iron in Plants
Authors: Marzorati Francesca , Midali Alessia , Morandini Piero , Murgia Irene
Published: May 2022

What can you do when you feel hungry? You could go to the kitchen and prepare a sandwich, or you could buy yourself a snack. But what if you are fixed to the ground? Plants are champions at reaching food that is far away from them while they are standing still. Below a plant, roots branch out into the soil in many directions, looking for the nutrients plants need for survival and growth. Iron is a very important nutrient for plants because it is essential for growth and development, and it also helps plants to face stresses in the environment. Even if iron availability in soil is very low, plants have developed two strategies for efficiently taking up iron and storing it. In this article, we will explore the importance of iron in plants’ lives, explaining how plants take up it and how balanced iron levels are important for plants’ (and our) survival.
Er,Cr:YSGG Laser Therapy for Drug-Induced Gingival Overgrowth: A Report of Two Case Series
Authors: Liu Yumei , Peng Qian , Liu Binjie , Wang Zhibin , Cao Qiong
Published: May 2022

Background

Drug-induced gingival overgrowth is common but neglected in patients with systemic disease medications until it seriously affects the quality of life.

Methods

Initial periodontal treatment, combined with water laser surgery, was performed sequentially in two cases.

Results

The therapeutic effect was good, and there was no recurrence along with good oral hygiene.

Conclusion

Water laser equipment surgery, as well as initial periodontal treatment, required that surgeons are trained specifically. A tool was devised for various oral diseases, and it was safer, more efficient and more comfortable than others.
Assessing the Impact of Youth-in-Agribusiness Program on Poverty and Vulnerability to Poverty in Nigeria
Authors: Bello Lateef Olalekan , Baiyegunhi Lloyd J. S. , Danso-Abbeam Gideon , Ogunniyi Adebayo Isaiah , Olagunju Kehinde , Abdoulaye Tahirou , Manyong Victor , Bamba Zoumana , Awotide Bola Amoke
Published: May 2022

Poverty persists in many developing countries, including Nigeria, owing to inadequate infrastructure, unemployment, or poor working conditions, among other factors. Youth poverty and vulnerability to poverty have been identified to prevalent among the young population. Using an endogenous switching probit regression approach, in this study, we evaluated the impacts of youth participation in agribusiness programs (YIAPs) on poverty and vulnerability to poverty in Nigeria. Our findings revealed that some demographic and institutional factors significantly influence poverty and vulnerability to poverty among youth. The impact estimates indicate that participation in an agribusiness program has a significant positive effect on poverty reduction among youth. Moreover, there would have been about a 28% reduction in exposure to future poverty for non-participants had they participated in a YIAP. Our results suggest that intervention programs, such as YIAPs, that focus on skill acquisition and youth empowerment should be strengthened and scaled-up in order to improve youth welfare and subsequently reduce/eradicate poverty and vulnerability to poverty among youth.
Being a Farmer in Austria during COVID-19—A Qualitative Study on Challenges and Opportunities
Authors: Meixner Oliver , Quehl Henriette Elisabeth , Pöchtrager Siegfried , Haas Rainer
Published: May 2022

To assess the effects of COVID-19 on Austrian farmers, a qualitative study design including computer-aided, qualitative content analysis was applied. Interviews with 34 Austrian farmers covering a broad spectrum of the sector were conducted to identify the impacts, obstacles, and opportunities during the first year of the COVID-19 pandemic. The results show that the diverse and heterogeneous impacts of COVID-19 on farms created significant challenges but also opportunities. Negative impacts included sales difficulties due to the closure of the hospitality industry and farmers markets, the ban on non-agricultural activities, the disrupted availability of workers and agricultural inputs, and the related additional workload. Mitigation of negative effects through political measures and interest groups showed limited effectiveness. Nevertheless, the functionality of the agricultural sector was maintained. During the initial phase of the pandemic, direct impacts on agricultural production were minimal and adjustments were adequate. There were also positive effects on parts of the agricultural sector, such as a boost in direct sales, increased demand in the food retail sector, and improved public perception of agriculture and farmers. Long-term changes in farms have been triggered and/or reinforced by the COVID-19 pandemic, mainly driven by digitization, investments in product and/or process innovations, and adjustments concerning marketing.
Management of Al3+ Residue in the Soil by Mapping Soil Capability in Retaining and Transporting Al3+ in the Farmland of Trang Bom District, Vietnam
Authors: Nguyen Anh , Nguyen Truc T. , Nguyen Dang Khue , Richter Otto , Do Huyen Thi Thu
Published: May 2022

The assessment of soil capability in retaining and transporting chemical substances is necessary, especially currently, with the overuse of chemical products for crop production. Depending on the soil properties, these chemicals may bound on soil particles or release and transport in the soil solution. In this study, we developed maps of the capability of soil to retain and transport Al3+, thereby evaluating the main soil factors affecting Al3+ fate in the agricultural land of Trang Bom District, Dong Nai Province, Vietnam. Information and data of the factors slope, soil texture, pH, organic matter, and ferrallitisation were processed and analyzed. The GIS tool was applied in combination with the analytical hierarchical process (AHP) to create the maps. Four hundred simulation runs were performed for criteria weight sensitivity analysis to explore the dependency of the resultant maps on the weights of the input factors. Sampling soil data were used to validate the accuracy of information given by the resultant maps. Results from the two maps show that the soils in the area have high capability in retaining and transporting Al3+. Ninety nine percent of the soils in the area have medium to high capability of Al3+ retention and about 65% of the soils have medium to high capability of transporting Al3+. For the agricultural land, about 65% of the land ranked as having a high to very high soil Al3+ retention capability and about 58% of the land ranked as having a medium to high capability of transporting Al3+. These maps can support the process of decision-making in identifying the appropriate dose and frequency of the chemical products that are applied on each soil capability zone; in this case study, the products contain aluminum. The accumulation of Al3+ in the soil, especially in the high Al3+ retention capability soil, can cause soil degradation and can cause negative effects on plant growth.
Tall Fescue (Festuca arundinacea Schreb.) Shows Intraspecific Variability in Response to Temperature during Germination
Authors: Ahmed Lina Q. , Escobar-Gutiérrez Abraham J.
Published: May 2022

Tall fescue is a major species growing in temperate grasslands. It is a cool-season perennial native of Western Europe and used worldwide as forage for its quality and adaptability to various soils and climates. By its effects on germination and seedling growth, temperature affects the recruitment of individuals and, consequently, the genetic diversity of plant communities. Under most climate change scenarios, breeding cultivars adapted to new ranges of temperature will be necessary. Knowing the variability of the responses to temperature by different accessions is an essential first step towards such breeding. In this work, we (i) analyze the intraspecific variability of tall fescue in response to a constant temperature during germination and (ii) quantitatively describe the response curves. A sample of nine, from 128, accessions of tall fescue was evaluated. Four replicates of 100 seeds per accessions were tested for germination in the dark at eight constant temperatures ranging from 5 to 40 °C with increases of 5 °C. The germinability, lag to start and maximum germination rates were estimated. It was observed that the responses of tall fescue accessions were statistically different (p < 0.05). The optimal temperature for maximum germination ranged from 9 °C to 25 °C. Germination was not observed for any accession at 40 °C. The novelty of this work comes from the duration of the sampling period at low temperatures that was longer than in most published papers. Based on the responses to temperature during germination, our findings suggest that a high intraspecific genetic variability exists in tall fescue that merits further exploration. This variability should be useful to breed new cultivars adapted to the new environmental conditions imposed by the ongoing fast climate change.
Effects of Years of Rice Straw Return on Soil Nitrogen Components from Rice–Wheat Cropped Fields
Authors: Cui Siyuan , Zhu Xinkai , Cao Guangqiao
Published: May 2022

Straw return is an important farmland management practice that influences the activity of soil nitrogen. Few studies have examined the distribution of soil nitrogen and its components in wheat–rice cropping fields in subtropical China. This study assesses the influence of different years of straw return on the distribution and variation of total soil nitrogen (TN), light fraction nitrogen (LFN), heavy fraction nitrogen (HFN), particulate nitrogen (PN), and mineral-bound nitrogen (MN). We conducted a field experiment with eight years of straw retention treatments in 2017 (no straw retention, NR; 1 year of straw retention, SR1; 2 years of straw retention, SR2; 3 years of straw retention, SR3; 4 years of straw retention, SR4; 5 years of straw retention, SR5; 6 years of straw retention, SR6; 7 years of straw retention, SR7) and one more treatment in 2018 (8 years of straw retention, SR8) in a rice–wheat cropping system at Yangzhou University Experimental Station in China. The results demonstrated that as the number of years of treatment increases, the content of TN, LFN, HFN, PN, and MN at each soil layer gradually increases. Compared with NR, the highest increase in TN, LFN, HFN, PN, and MN under SR1-SR8 in the 0–20 cm soils was 38.10%, 150.73%, 35.61%, 79.97%, and 27.71%, respectively, but increases in TN, HFN, and MN content gradually slowed after six years of straw return. The contents or variation of TN were extremely significantly correlated (p < 0.01) with that of LFN, HFN, PN, and MN, while LFN had the highest variation. In general, straw return could improve the quality of the 0–20 cm nitrogen pool. LFN was the best indicator of changes to the soil nitrogen pool affected by years of straw return.
Comparative Histology of C Thyrocytes in Four Domestic Animal Species: Dog, Pig, Horse, and Cattle
Authors: Sokołowska Justyna , Cywińska Anna , Puchalska Martyna
Published: May 2022

The number, morphology, and distribution of C thyrocytes within the thyroid gland vary among species; however, studies in domestic animals are limited. In this study we compared the morphology, distribution pattern, and percentage of C thyrocytes in four domestic species: dogs, pigs, horses, and cattle. Eighty thyroid glands, 20 per species, were examined. C thyrocytes were visualized immunohistochemically with anti-calcitonin rabbit polyclonal antibody alone and combined with the periodic acid Schiff method to simultaneously visualize C thyrocytes with the basement membranes of thyroid follicles. C thyrocyte morphology varied considerably between species, from oval- (dogs) and spindle-shaped (pigs) to polymorphic (cattle and horses). Bovine C thyrocytes demonstrated cytoplasmic protrusion. C thyrocytes were located intrafolliculary (all species), epifollicularly (dogs, horses, cattle), or interfolicularly (cattle). Most porcine and bovine C thyrocytes existed individually whereas canine C thyrocytes usually formed clusters. In horses, they tended to form groups of various shapes and sizes or even rims encompassing whole follicles. In all species, the number of C thyrocyte profiles increased from the periphery to the central area of the thyroid lobe. The mean total fraction of C thyrocytes in the superficial, intermediate, and central areas were as follows: 2.55%, 8.43%, and 12.48% in dogs; 3.81%, 7.66%, and 10.79% in pigs; 1.55%, 7.44%, and 8.87% in horses; and 2.62%, 10.75%, and 12.96% in cattle. No statistical differences in the total number of C thyrocyte profiles were observed among species (8.87% in dogs, 8.58% in cattle, 7.98% in pigs, and 5.83% in horses). Our results indicated that the studied species displayed their own morphological characteristics and distribution pattern of C thyrocytes; however, total numbers of C thyrocyte profiles and their localization within the thyroid lobe are comparable.
The COVID-19 Animal Fostering Boom: Ephemera or Chimera?
Authors: Reese Laura A. , Jacobs Jacquelyn , Gembarski Jordan , Opsommer Caden , Walker Bailey
Published: May 2022

There has been discussion in traditional and social media about increases in the numbers of people willing to foster animals in their homes during the pandemic. However, there is a lack of empirical data on whether that increase was a temporary response to the stress of COVID-19 or the ability to work from home, if it might have lasting effects, or indeed, whether an increase occurred at all. Using a national survey of over 600 animal shelter/rescue foster volunteers it appears that fostering did increase during the pandemic (x2 = 45.20, p = 0.00), particularly among volunteers working from home, those with higher education, those that were younger and male, and those that did not have their own dog. The study concludes that there was an increase in fostering but that the impact is likely to be ephemeral predicated on the ability to work from home. Organizations may be able to retain foster volunteers through support, particularly emotional support, directed at the human as opposed to focusing solely on the dog.
Pregnancy in Slaughtered Lambs and Sheep—A Cross-Sectional Study in Three Abattoirs in Switzerland
Authors: Pagamici Chiara , Stephan Roger
Published: May 2022

The slaughter of pregnant sheep and goats is not restricted in Switzerland. The aim of this study was to assess the prevalence of pregnant sheep and lambs being slaughtered in Switzerland and to determine the state of gestation and vital signs of the fetuses in order to assess the need to take measures and raise awareness of this issue. The data collection was carried out from March 2021 to February 2022, comprising 115 days in three abattoirs. A total of 18,702 sheep and lambs were included in this cross-sectional study, and 8770 were female (46.9%), 663 of which were pregnant at slaughter (7.6%). The pregnancy rate varied by age category: 404 lambs (6.1%) and 259 sheep (11.9%) were pregnant. The highest pregnancy rate was found in winter (25.7%). Among the 663 pregnancies, more than a quarter were multiple pregnancies (28.2%). A total of 169 animals were in the third trimester of pregnancy (25.5%), where living fetuses were mainly found (81.1%). As it cannot be definitively ruled out that fetuses feel conscious pain, the data from this study underline that, from an ethical point of view, there is a need for action and that measures must be taken to reduce the number of pregnant slaughtered animals.
Supplementation with Fermented Feedstuff Enhances Orexin Expression and Secretion Associated with Increased Feed Intake and Weight Gain in Weaned Pigs
Authors: Lu Yang , Zhang Ruiyang , Lei Hulong , Hang Yiqiong , Xue Huiqin , Cai Xuan , Lu Yonghong
Published: May 2022

The health status of weaned pigs is crucial for their subsequent growth performance. Supplementation with fermented feedstuff is able to improve the feed intake and growth of weaned pigs; however, the exact mechanism behind this is not clear. Hence, in the present study a total of 320 Duroc × Landrace × Yorkshire weaned pigs were selected and allocated to the following two groups: unfermented diet group (UFD) and fermented diet group (FD). The experimental period lasted 21 days. At the end of the experiment, feces, blood, and gastrointestinal tissue samples (including the stomach, jejunum, and ileum) were collected and used for further analysis. The results of growth performance suggested that the FD group had significantly increased (p < 0.05) average daily feed intake (ADFI) and average daily gain (ADG) during the first week, during the last two weeks, and over the entire three-week period compared with the UFD group. The results of the apparent nutrient digestibility of pigs showed that, compared with the UFD group, the FD group showed increased phosphorus (p < 0.05) and CP (p < 0.1) digestibility. There were no significant differences in the serum biochemical parameters between the UFD and FD groups. Moreover, our results showed that the FD group showed significantly increased gene expression of SGLT1 and PepT1 in the jejunum (p < 0.05). Compared with the UFD group, the FD group showed an increased (p < 0.05) serum orexin level and prepro-orexin (PPOX) expression in the gastric fundus, jejunum, and ileum mucosa and increased IGF-1 and IGFR expression in the jejunum. Collectively, these results indicated that supplementation with fermented feedstuff in the diet effectively enhanced the feed intake and growth of weaned pigs and that this may have been caused by the increased orexin, IGF-1, and IGFR serum levels.
Mechanisms Determining Body Size and Shape Difference in Algerian Spur-Thighed Tortoises (Testudo graeca)
Authors: Tiar-Saadi Manel , Tiar Ghoulem , Bouslama Zihad , Široký Pavel
Published: May 2022

Using data for the body size and shell shape of Algerian Testudo graeca, we assessed how proximate causes shaped the observed variation in the morphology of adults. All of the studied populations displayed significant sexual size and shape dimorphisms. Relative to body length, females displayed larger, more voluminous and domed shells than males. We found clear evidence that variation in body size at maturity influenced sexual size dimorphism. Body size at maturity depends on the duration of growth from hatching up to the point of reaching sexual maturity. In the studied populations, sexual maturity, estimated by counting growth lines, was always reached earlier in males than in females (a time difference of 1.4–3.0 years). Similar to sexual size dimorphism, geographic variation in adult body sizes was also influenced by variations in the corresponding sizes at maturity. Remarkably, the population with the largest tortoises had the latest mean maturation time: 9.1 for males and 10.5 for females. Thus, the later completion of maturation was a determinant for a larger size in adulthood. The largest tortoises among the studied populations were measured at the Djelfa locality, where the recorded sizes of males and females reached 186 and 230 mm, respectively.
Boar Semen Shipping for Artificial Insemination: Current Status and Analysis of Transport Conditions with a Major Focus on Vibration Emissions
Authors: Hafemeister Tim , Schulze Paul , Bortfeldt Ralf , Simmet Christian , Jung Markus , Fuchs-Kittowski Frank , Schulze Martin
Published: May 2022

In the modern pig reproduction system, artificial insemination (AI) doses are delivered from AI centers to sow farms via logistics vehicles. In this study, six breeding companies in three countries (Brazil, Germany, and the USA) were interviewed about their delivery process. It was found that there is currently no comprehensive monitoring system for the delivery of semen. The entire process “shipping of boar semen” was documented using Business Process Model and Notation (BPMN). Although it is not currently known which vibrations occur at all, it is suspected that vibration emissions affect the quality of boar semen. For this reason, a prototype of a measuring system was developed to calculate a displacement index (Di), representing vibration intensities. Vibrations were analyzed in standardized road trials (n = 120) on several road types (A: smooth asphalt pavement, B: rough asphalt pavement, C: cobblestone, and D: dirt road) with different speeds (30, 60, 90, 120, and 150 km/h). A two-way ANOVA showed significant differences in mean Di, depending on road surface and speed as well as an interaction of both factors (p < 0.001). A field study on a reference delivery from a German AI center to several sow farms indicated that 33% of the observed roads are in good quality and generate only a few vibrations (Di ≤ 1), while 40% are of a moderate quality with interrupted surfaces (Di = 1–1.5). However, 25% of the roads show markedly increased vibrations (Di ≥ 1.5), as a consequence of bad conditions on cobblestones or unpaved roads. Overall, more attention should be paid to factors affecting sperm quality during transport. In the future, an Internet of Things (IoT) based solution could enable complete monitoring of the entire transport process in real time, which could influence the courier’s driving behavior based on road conditions in order to maintain the quality of the transported AI doses.
Characterization of Longitudinal Testosterone, Cortisol, and Musth in Male Asian Elephants (Elephas maximus), Effects of Aging, and Adrenal Responses to Social Changes and Health Events
Authors: Glaeser Sharon S. , Edwards Katie L. , Paris Stephen , Scarlata Candace , Lee Bob , Wielebnowski Nadja , Finnell Shawn , Somgird Chaleamchat , Brown Janine L.
Published: May 2022

The conservation of endangered species and sustainability of managed populations requires considerations to ensure the health and welfare of individuals. Male elephants experience a biological phenomenon called “musth”, which is characterized by increased testosterone production, temporal gland secretion and urine dribbling, heightened aggression and sexual behavior, and therefore can pose unique challenges for human safety and animal welfare. This study characterized longitudinal (9 to 22 years) patterns of circulating testosterone and cortisol in relation to musth in four adult Asian elephant bulls spanning ages from 12 to 54 years. Age-related effects on musth activity and adrenal responses to social changes and clinical health events were also examined. All bulls exhibited regular annual musth cycles. Circulating cortisol covaried positively with testosterone and musth, highlighting intrinsic patterns that should be considered when evaluating the impact of social, health, and environmental changes on adrenal glucocorticoid activity. Except for an end-of-life cortisol increase in one bull, there was no clear evidence of chronically elevated cortisol secretion outside of musth in any individual. Testosterone decreased with age in sexually mature bulls, whereas age-related changes in cortisol varied across individuals, with the three older bulls showing the greatest rate of change during musth versus inter-musth periods. In contrast to physiological factors, there was no evidence of social factors, such as addition of a new male and death of male herdmates, impacting adrenal glucocorticoid activity in these bulls in the short term. Changes in cortisol were associated with treatment for Mycobacterium tuberculosis (M. tb) in two bulls, increasing after start of treatment and decreasing with cessation of treatment, but were not clearly associated with activation of disease. This study highlights the importance of longitudinal hormone monitoring to track changes in physiological function and responses to social, health, and environmental change in elephant bulls, which is important for making more informed decisions on how to manage male elephants under varying degrees of human care to ensure welfare and safety.
Dietary Crude Fiber Levels for Optimal Productivity of Male Ross 308 Broiler and Venda Chickens Aged 1 to 42 Days
Authors: Ginindza Muzi , Mbatha Khanyisile R. , Ng’ambi Jones
Published: May 2022

The study determined the effects of dietary crude fiber (CF) levels on the production performance of male Ross 308 broiler and indigenous Venda chickens. There were 360 male Ross 308 broiler and male Venda chickens aged 1–21 for Phase 1 and 196 chickens aged 22–42 days for Phase 2. Chickens were allocated four diets with different levels of CF (3, 4, 5, and 7%) in a completely randomized design. Quadratic regression analyses were used to determine the dietary CF levels for the optimal production responses. In Phase 1, the feed intake, growth, live weight, nitrogen retention, and metabilizable energy (ME) intake of the Ross 308 broiler chickens were improved at dietary CF levels of 3.9, 4.5, 4.5, 3.2, and 3.7%, respectively. In the Venda chickens, feed intake, growth, live weight, nitrogen retention, and ME intake were optimized at 4.4, 4.8, 4.7, 4.1, and 3.3% CF, respectively. In Phase 2, the feed intake, nitrogen retention, and neutral detergent fiber digestibility (NDFD) were enhanced at CF levels of 6.4, 4.4, and 3.7% in the Ross 308 broiler chickens, respectively. Dietary CF levels of 4.5, 5.8, 5.7, 5.1, 3.9, and 4.4% optimized the feed intake, growth rate, live weight, nitrogen retention, NDF, and ADFD, respectively, in Venda chickens. It was concluded that the indigenous Venda chickens coped better with higher dietary CF than the Ross 308 broiler chickens.
Horses’ Response to a Novel Diet: Different Herbs Added to Dry, Wet or Wet-Sweetened Oats
Authors: Stachurska Anna , Tkaczyk Ewelina , Różańska-Boczula Monika , Janicka Wiktoria , Janczarek Iwona
Published: May 2022

The commercial horse feed industry uses palatants to mask undesirable tastes of feeds and enhance product acceptance. However, an unknown odour or taste may also hinder feed intake, due to, inter alia, novelty. The objective of the study was to assess the horses’ response to novel diet: five different herbs added alternately to dry, wet or wet-sweetened oats. Twenty adult horses were given different diet combinations of a feed presentation and a herb: field mint, common yarrow, common chamomile, common sage and common nettle, consecutively, once daily. The response to novelty was assessed regarding traits showing the willingness to consume: times of olfaction and consumption, times and numbers of intervals in consumption and drinking water, and the mass of leftovers. The results show that properties of the herbs studied did not hinder the consumption and only the odour of the dry common sage delayed the intake. Wetting or wetting and sweetening the diet accelerated the intake. In conclusion, herbs in small amounts do not significantly affect the willingness to consume feed. Although wet and wet-sweetened diet presentations may be novel to horses, they increase the feed palatability and can be suggested for use when preparing horse diets.