bluuebunny/crossref_metadata_2025 huggingface dataset:
IL-1 and TNF mediates IL-6 signaling at the maternal-fetal interface during intrauterine inflammation
Authors: Presicce Pietro , Roland Cynthia , Senthamaraikannan Paranthaman , Cappelletti Monica , Hammons McKensie , Miller Lisa A. , Jobe Alan H. , Chougnet Claire A. , DeFranco Emily , Kallapur Suhas G.
Published: June 2024

Introduction

IL6 signaling plays an important role in triggering labor and IL6 is an established biomarker of intrauterine infection/inflammation (IUI) driven preterm labor (PTL). The biology of IL6 during IUI at the maternal-fetal interface was investigated in samples from human subjects and non-human primates (NHP).

Methods

Pregnant women with histologic chorioamnionitis diagnosed by placenta histology were recruited (n=28 term, n=43 for preterm pregnancies from 26-36 completed weeks of gestation). IUI was induced in Rhesus macaque by intraamniotic injection of lipopolysachharide (LPS, n=23). IL1 signaling was blocked using Anakinra (human IL-1 receptor antagonist, n=13), and Tumor necrosis factor (TNF) signaling was blocked by anti TNF-antibody (Adalimumab n=14). The blockers were given before LPS. All animals including controls (intraamniotic injection of saline n=27), were delivered 16h after LPS/saline exposure at about 80% gestation.

Results

IUI induced a robust expression of IL6 mRNAs in the fetal membranes (chorion-amnion-decidua tissue) both in humans (term and preterm) and NHP. The major sources of IL6 mRNA expression were the amnion mesenchymal cells (AMC) and decidua stroma cells. Additionally, during IUI in the NHP, ADAM17 (a protease that cleaves membrane bound IL6 receptor (IL6R) to release a soluble form) and IL6R mRNA increased in the fetal membranes, and the ratio of IL6 and soluble forms of IL6R, gp130 increased in the amniotic fluid signifying upregulation of IL6 trans-signaling. Both IL1 and TNF blockade suppressed LPS-induced IL6 mRNAs in the AMC and variably decreased elements of IL6 trans-signaling.

Discussion

These data suggest that IL1 and TNF blockers may be useful anti-inflammatory agents via suppression of IL6 signaling at the maternal-fetal interface.
Bioprospecting and marine ‘omics’: surfing the deep blue sea for novel bioactive proteins and peptides
Authors: Moutinho Cabral Inês , Gonçalves Cátia , Grosso Ana R. , Costa Pedro M.
Published: June 2024

The vast biological and biochemical diversity of the global ocean is the driver behind marine bioprospecting for novel bioproducts. As Marine Biotechnology is gaining momentum as one of the main pillars of the ‘Brue Growth’ revolution, the ability to screen for novel compounds of interest in species with little or no genomic resources is paramount. With this respect, proteins, which are easily metabolised, can be synthetised using convenient DNA recombinant methods and can easily be modified to better meet the needs of human society, making them prized targets. Evidently, proteins that hold natural bioactivity and specificity such as toxins and other venom components, have long captured the focus of biotechnologists, leading to the merger between environmental omics and toxinology termed as ‘venomics’. Indeed, bioactive proteins such as conopeptides, conotoxins, turripeptides and others are long deemed important subjects of research. Even though current mainstream paradigms set the focus on secondary metabolites from marine organisms, transcriptomics and proteomics approaches and their combination are rising strategies for screening for thousands of proteins and peptides in non-conventional biological models, emphasising, but not limited to, marine invertebrate animals due to their abundance, biodiversity and uncanny biochemical strategies to cope with selective pressure in literally every known marine habitat. Untargeted approaches, such as RNA-Seq – based transcriptomics and tandem mass spectrometry – based proteomics, can circumvent limitations related with absent or reduced genomic annotation. The present review will outline the main contributions of ‘omics’ and computational approaches for bioprospecting for proteinaceous marine bioactives. Despite the relatively low number of ‘omics’ studies with the main purpose of discover novel compounds, there is already important literature showcasing pipelines and approaches for revolutionising the exploration of the ocean.
Transcriptome profiling of the posterior salivary glands of the cuttlefish Sepia officinalis from the Portuguese West coast
Authors: Gonçalves Cátia , Moutinho Cabral Inês , Alves de Matos António P. , Grosso Ana R. , Costa Pedro M.
Published: June 2024

Cephalopods like octopuses and cuttlefishes are known to secrete a ‘toxic saliva’ to inject into their prey, especially crustaceans since the XIX century. However, only in the mid-XX century were the first coleoid-specific toxins successfully isolated. Motivated by the growing interest on the global ocean as an almost inexhaustible source of novel bioactive compounds, we used RNA-Seq – based transcriptomics and de novo assembly of transcriptomes to screen the posterior salivary glands of Sepia officinalis (the common cuttlefish) from the Portuguese West coast for toxins and other bioactive proteins and peptides. Supported by microanatomical analyses, the posterior salivary glands constitute indeed the ‘venom gland’ whereas the more elusive anterior salivary glands (embedded in the buccal mass) are responsible for the production of mucin-rich saliva that is effectively the vehicle that transports the toxins as the venom is injected into the prey. Indeed, the transcriptomic profiling suggests that the cuttlefish venom is complex mixture of bioactive proteins, among which neurotoxins are major players, together with enzymes whose function is to digest the extracellular matrix to facilitate diffusion of the toxins. Nonetheless, by comparing with previous RNA-Seq data obtained from S. officinalis collected from other biogeographical areas, it may be suggested that significant inter-populational variation in venom composition can occur, which may potentially increase the span of bioactives secreted by these animals. We isolated and validated the full coding sequences for three important toxins, a cysteine-rich venom protein (CRVP), a venom insulin (VIns) and a cephalotoxin (CTX). The toxins seem to be relatively conserved among coleoids but diverging from other venomous mollusks such as cone snails. Their properties as potent modulators of glucose (in the case of VIns) and as potential neurotoxins (like CRVP and CTX) can render them primer targets for drug development.
UV photodegradation of methylene blue using microstructural carbon materials derived from citrullus colocynthis
Authors: Bhatti Muhammad Ali , Dawi Elmuez , Tahira Aneela , Hulio Ahmed Ali , Halepoto Imran Ali , Chang Sajjad Ali , Solangi Abdul Ghaffar , Nafady Ayman , Tonezzer Matteo , Haj Ismail Abd Al Karim , Ibupoto Zafar Hussain
Published: June 2024

A low temperature aqueous growth followed by mild pyrolysis was used in this study to synthesize high-quality carbonized materials from the deserted plant Citrullus Colocynthis. It was found that the carbon material prepared for this study contained an abundance of functional groups and surface active sites. A few microns were evidently the size of the carbon material. This study investigated a variety of photocatalytic performance evaluation parameters, including initial dye concentration of methylene blue, pH effect on dye solution, scavenger stability, and recycle stability via irradiating UV light. Methylene blue degradation was found to be significantly affected by pH and concentration of the dye solution. It has been found that pH five is the most effective pH for the removal of dyes. As a result of the study, we found that methylene blue decays according to pseudo first order kinetics and is estimated to remove dye at an almost 100% rate.
Causal effects of gut microbiota on chalazion: a two-sample Mendelian randomization study
Authors: Zhang Wenfei , Gu Xingwang , Zhao Qing , Wang Chuting , Liu Xinyu , Chen Youxin , Zhao Xinyu
Published: June 2024

Purpose

To investigate the causal relationship between gut microbiota (GM) and chalazion through Mendelian randomization (MR) analysis.

Methods

GM-related genome-wide association studies (GWAS) were obtained from the International Consortium MiBioGen. Genetic data for chalazion were sourced from the MRC Integrative Epidemiology Unit (IEU) Open GWAS database. Five MR methods, including inverse variance weighted (IVW), were employed to estimate causal relationships. Cochran’s Q test was used to detect heterogeneity, the MR-Egger intercept test and MR-PRESSO regression were utilized to detect horizontal pleiotropy, and the leave-one-out method was employed to validate data stability.

Results

We identified 1,509 single nucleotide polymorphisms (SNPs) across 119 genera as instrumental variables (IVs) (p < 1 × 10−5). According to the inverse variance weighted (IVW) estimate, the Family XIII AD3011 group (OR = 1.0018, 95% CI 1.0002–1.0035, p = 0.030) and Catenibacterium (OR = 1.0013, 95% CI 1.0002–1.0025, p = 0.022) were potentially associated with increased risk of chalazion. Conversely, Veillonella (OR = 0.9986, 95% CI 0.9974–0.9999, p = 0.036) appeared to provide protection against chalazion. There was no evidence of heterogeneity or pleiotropy.

Conclusion

This study uncovered the causal relationship between GM and chalazion, pinpointing Catenibacterium and Family XIII AD3011 group as potential risk contributors, while highlighting Veillonella as a protective factor. In-depth investigation into the potential mechanisms of specific bacteria in chalazion was essential for providing novel therapeutic and preventive strategies in the future.
Conservation tillage: a way to improve yield and soil properties and decrease global warming potential in spring wheat agroecosystems
Authors: Sadiq Mahran , Rahim Nasir , Tahir Majid Mahmood , Alasmari Abdulrahman , Alqahtani Mesfer M. , Albogami Abdulaziz , Ghanem Kholoud Z. , Abdein Mohamed A. , Ali Mohammed , Mehmood Nasir , Yuan Jianyu , Shaheen Aqila , Shehzad Muhammad , El-Sayed Mohamed H. , Chen Guoxiang , Li Guang
Published: June 2024

Climate change is one of the main challenges, and it poses a tough challenge to the agriculture industry globally. Additionally, greenhouse gas (GHG) emissions are the main contributor to climate change; however, croplands are a prominent source of GHG emissions. Yet this complex challenge can be mitigated through climate-smart agricultural practices. Conservation tillage is commonly known to preserve soil and mitigate environmental change by reducing GHG emissions. Nonetheless, there is still a paucity of information on the influences of conservation tillage on wheat yield, soil properties, and GHG flux, particularly in the semi-arid Dingxi belt. Hence, in order to fill this gap, different tillage systems, namely conventional tillage (CT) control, straw incorporation with conventional tillage (CTS), no-tillage (NT), and stubble return with no-tillage (NTS), were laid at Dingxi, Gansu province of China, under a randomized complete block design with three replications to examine their impacts on yield, soil properties, and GHG fluxes. Results depicted that different conservative tillage systems (CTS, NTS, and NT) significantly (p < 0.05) increased the plant height, number of spikes per plant, seed number per meter square, root yield, aboveground biomass yield, thousand-grain weight, grain yield, and dry matter yield compared with CT. Moreover, these conservation tillage systems notably improved the soil properties (soil gravimetric water content, water-filled pore space, water storage, porosity, aggregates, saturated hydraulic conductivity, organic carbon, light fraction organic carbon, carbon storage, microbial biomass carbon, total nitrogen, available nitrogen storage, microbial biomass nitrogen, total phosphorous, available phosphorous, total potassium, available potassium, microbial counts, urease, alkaline phosphatase, invertase, cellulase, and catalase) while decreasing the soil temperature and bulk density over CT. However, CTS, NTS, and NT had non-significant effects on ECe, pH, and stoichiometric properties (C:N ratio, C:P ratio, and N:P ratio). Additionally, conservation-based tillage regimes NTS, NT, and CTS significantly (p < 0.05) reduced the emission and net global warming potential of greenhouse gases (carbon dioxide, methane, and nitrous oxide) by 23.44, 19.57, and 16.54%, respectively, and decreased the greenhouse gas intensity by 23.20, 29.96, and 18.72%, respectively, over CT. We conclude that NTS is the best approach to increasing yield, soil and water conservation, resilience, and mitigation of agroecosystem capacity.
p53 deficiency mediates cisplatin resistance by upregulating RRM2 and crotonylation of RRM2K283 through the downregulation of SIRT7
Authors: Sun Liangjie , Li Yi , Wang Meng , Luo Lan , Sun Ruiqing , Chen Yang , Bai Yan , Ding Chong , Wang Yixiang
Published: June 2024

p53 deficiency plays a crucial role in chemotherapy resistance through various biological events, including posttranslational modifications (PTMs). Recently, lysine crotonylation (Kcr) has been shown to play a vital role in cancer progression. However, the global p53-regulated crotonylome and the function of these altered Kcr proteins after p53 deficiency remain unclear. In this study, we used a SILAC-based quantitative crotonylome to identify 3,520 Kcr in 1924 crotonylated proteins in response to p53 knockout. We found that increased crotonylation of RRM2 at K283 (RRM2K283Cr) in the presence of p53 deficiency promoted HCT116 cell resistance to cisplatin. We discovered that SIRT7 could be the decrotonylase of RRM2 and was downregulated after p53 knockout, resulting in increased RRM2K283Cr. Mechanistically, p53 deficiency inhibited cell apoptosis by upregulating RRM2 protein expression and RRM2K283Cr-mediated cleaved-PARP1 and cleaved-caspase3 expression, and SIRT7 was downregulated to upregulate crotonylation of RRM2 upon p53 deficiency. In conclusion, our results indicated that p53 deficiency plays a malignant role in colon cancer resistance to cisplatin therapy by regulating RRM2 protein and RRM2K283Cr expression. Our findings provide a novel therapeutic target against p53-deficient cancer.
Sleep and sleep-related breathing disorders in patients with spinal muscular atrophy: a changing perspective from novel treatments?
Authors: Abati Elena , Mauri Eleonora , Rimoldi Martina , Madini Barbara , Patria Francesca , Comi Giacomo Pietro , Corti Stefania
Published: June 2024

Spinal Muscular Atrophy (SMA) is an inherited neuromuscular disorder characterized by progressive muscle weakness and atrophy, resulting from the degeneration of motor neurons in the spinal cord. A critical aspect of SMA is its impact on respiratory function. As the disease progresses, respiratory muscles, in particular intercostal muscles, become increasingly affected, leading to breathing difficulties and respiratory failure. Without intervention, many children with SMA type 1 die from respiratory failure before their second year of life. While assisted ventilation has improved survival, it often results in ventilator dependence. The development of new SMN-augmenting therapies has renewed optimism, but their long-term impact on respiratory function is uncertain, and non-invasive respiratory support remains an important part of SMA management. Despite the importance of respiratory support in SMA, knowledge regarding sleep disorders in this population is limited. This review aims to synthesize existing literature on sleep and sleep-related breathing disorders in patients with SMA, with a focus on SMA type 1. We summarize evidence of sleep-disordered breathing and respiratory failure in SMA, as well as outcomes and survival benefits associated with non-invasive or invasive ventilation with or without pharmacological therapies. We also discuss current knowledge regarding the effects of novel disease-modifying therapies for SMA on respiratory function and sleep. In conclusion, optimal care for children with SMA requires a multidisciplinary approach that includes neurology and respiratory specialists. This review highlights the importance of monitoring sleep and respiratory function in SMA, as well as the potential benefits and challenges associated with assisted ventilation combined with new therapies.
Transcranial focused ultrasound to the posterior cingulate cortex modulates default mode network and subjective experience: an fMRI pilot study
Authors: Lord Brian , Sanguinetti Joseph L. , Ruiz Lisannette , Miskovic Vladimir , Segre Joel , Young Shinzen , Fini Maria E. , Allen John J. B.
Published: June 2024

Background

Transcranial focused ultrasound (TFUS) is an emerging neuromodulation tool for temporarily altering brain activity and probing network functioning. The effects of TFUS on the default mode network (DMN) are unknown.

Objective

The study examined the effects of transcranial focused ultrasound (TFUS) on the functional connectivity of the default mode network (DMN), specifically by targeting the posterior cingulate cortex (PCC). Additionally, we investigated the subjective effects of TFUS on mood, mindfulness, and self-related processing.

Methods

The study employed a randomized, single-blind design involving 30 healthy subjects. Participants were randomly assigned to either the active TFUS group or the sham TFUS group. Resting-state functional magnetic resonance imaging (rs-fMRI) scans were conducted before and after the TFUS application. To measure subjective effects, the Toronto Mindfulness Scale, the Visual Analog Mood Scale, and the Amsterdam Resting State Questionnaire were administered at baseline and 30 min after sonication. The Self Scale and an unstructured interview were also administered 30 min after sonication.

Results

The active TFUS group exhibited significant reductions in functional connectivity along the midline of the DMN, while the sham TFUS group showed no changes. The active TFUS group demonstrated increased state mindfulness, reduced Global Vigor, and temporary alterations in the sense of ego, sense of time, and recollection of memories. The sham TFUS group showed an increase in state mindfulness, too, with no other subjective effects.

Conclusions

TFUS targeted at the PCC can alter DMN connectivity and cause changes in subjective experience. These findings support the potential of TFUS to serve both as a research tool and as a potential therapeutic intervention.
Independent association between prostate-specific antigen nadir and PSA progression-free survival in first-line abiraterone acetate treatment in castration-resistant prostate cancer patients: a pilot study
Authors: Du Hong , Xie Wenjuan , Chen Wenqiang , Wang Yu , Liao Yong , Qiu Mingxing , Li Jun
Published: June 2024

Background

There is limited evidence regarding the correlation between prostate-specific antigen (PSA) kinetics and clinical outcomes. Therefore, after regulating other covariates, we studied patients with castration-resistant prostate cancer who received abiraterone acetate as the first-line treatment. In this study, we investigated whether time to PSA nadir was independently associated with PSA progression-free survival (PFS).

Methods

As a retrospective cohort study, this study contained a total of 77 castration-resistant prostate cancer patients who received abiraterone acetate from October 2015 to April 2021 in a Chinese hospital. The dependent variable was PSA-PFS. The objective independent variable was time to PSA nadir (TTPN). Covariates involved in this study included age, duration of androgen deprivation therapy (ADT), PSA level at baseline, time of 50% PSA decline, time of PSA decline to nadir, Gleason score, bone metastasis, previous treatment, PSA decline <50% in 3 months, PSA to nadir in 3 months, PSA decline <90%, PSA decline <0.2 ng/mL, and PSA flare.

Results

For the 77 subjects, their mean age was 72.70 ± 8.08 years. Fully calibrated linear regression findings indicated that PSA decline and kinetics were positively associated with PFS (months) after adjusting confounders (β = 0.77, 95% CI: 0.11–1.44). A non-linear relationship was not detected between PSA decline or PSA kinetics and progression-free survival.

Conclusion

According to the data of this study, there was a correlation between early PSA changes and patients treated with abiraterone acetate.
A bibliometric and visualization analysis of research trends and hotspots on targeted therapy for breast cancer from 2003 to 2022
Authors: Wu Deqi , Pan Chi , Hu Yangying , Shi Zhijie , Zhou Yankun , Xiao Min
Published: June 2024

Background

Breast cancer is a significant public health issue, exhibiting the most pronounced occurrence and fatality rates among malignant neoplasms globally. Targeted therapy is a medical intervention that focuses on specific molecular markers. This study aims to investigate and evaluate the current research trends and directions in the field of targeted therapy for breast cancer using bibliometric analysis.

Method

The Web of Science database was utilized to retrieve relevant articles published between 2003 and 2022. The VOSviewer software and Bibliometrix package in the R language were employed to conduct co-occurrence and clustering analyses of authors, countries, institutions, journals, references, and the CiteSpace tool was utilized for keyword burst detection.

Results

A total of 2,258 articles were included and the annual number of publications increased rapidly. The most prolific country on this topic was the USA (n=898, 39.77%) and the University of Texas MD Anderson Cancer Center published most papers (n=93). Dennis J. Slamon and Gabriel N. Hortobagyi stood out in the field, with Dennis J. Slamon leading in terms of co-citations(n=653) and Gabriel N. Hortobagyi topping the list in terms of published articles(n=18). The most productive journal was Breast Cancer Research and Treatment and the most cited journal was Journal of Clinical Oncology. The clustering of keywords indicated that the primary focus of researches in the past two decades was on the development and clinical evaluation of tumor-targeted drugs associated with the epidermal growth factor receptor (EGFR) family signaling pathway, and explored mechanisms related to biological behavior of breast cancer. Keywords co-occurrence and burst analysis identified current research hotspots and potential research trends.

Conclusion

This study employed bibliometric analysis to examine research on targeted therapy for breast cancer over a span of 20 years, and identified development trends of research and elucidated potential research trajectories in the domain of this topic. This study helps in the identification of prospective collaborators and partner institutions for researchers.
Allogeneic stem cell transplantation in multiple myeloma: is there still a place?
Authors: Liberatore Carmine , Fioritoni Francesca , Di Ianni Mauro
Published: June 2024

The introduction of novel agents dramatically improved response and outcomes of multiple myeloma (MM) and led to a sharp decline in the use of allogeneic hematopoietic stem-cell transplantation (allo-HSCT). Thus, recent guidelines do not recommend anymore allo-HSCT as consolidation in the first-line treatment of newly diagnosed MM, even in high-risk patients. In a relapsed/refractory setting, allo-HSCT is not routinely recommended but should only be performed within clinical trials in young and high-risk patients. Nonetheless, allo-HSCT still represents a potential curative approach that has been used for decades in the treatment of MM and plasma cell neoplasms with favorable results and may still represent a treatment option for carefully selected patients. Despite that promising results were obtained with CAR T-cell therapies and bispecific antibodies in triple- and penta-exposed/refractory MM, these patients will inevitably relapse. To date, less is known about outcomes of allo-HSCT in patients exposed to novel immunotherapeutic drugs. Therefore, allo-HSCT could represent a reasonable treatment choice for younger and high-risk patients who have relapsed after CAR T-cell therapies and bispecific antibodies as well as an alternative for patients not eligible to these treatments and in those countries where immunotherapies are not yet available. In the choice of conditioning, reduced intensity conditioning regimens are currently recommended for the lower toxicity and mortality. Moreover, the use of alternative donors, particularly haploidentical, has progressively increased in last years with results comparable to full matched donors. Finally, post-transplantation maintenance strategies are encouraged whenever feasible.
Complete androgen insensitivity syndrome coexisting with müllerian duct remnants: a case report and literature review
Authors: Chen De-lu , Guo Song , Chen Qiu-li , Qiu Shan-jiao , Xu Yu-ying , Zhang Jun , Ma Hua-mei , Li Yan-hong
Published: June 2024

This study represents the first documentation of the coexistence of complete androgen insensitivity syndrome (CAIS) with Müllerian duct remnants (MDRs) in mainland China. Additionally, we provide a comprehensive review of the existing literature concerning CAIS with MDRs resulting from androgen receptor (AR) gene mutations. This study broadens the clinical spectrum of CAIS and offer novel insights for further exploration into Müllerian duct regression. A 14-year-old patient, initially raised as female, presented to the clinic with complaints of “primary amenorrhea.” Physical examination revealed the following: armpit hair (Tanner stage 2), breast development (Tanner stage 4 with bilateral breast nodule diameter of 7 cm), sparse pubic hair (Tanner stage 3), clitoris measuring 0.8 cm × 0.4 cm, separate urethral and vaginal openings, and absence of palpable masses in the bilateral groin or labia majora. The external genital virilization score was 0 points. Serum follicle-stimulating hormone level was 13.43 IU/L, serum luteinizing hormone level was 31.24 IU/L, and serum testosterone level was 14.95 nmol/L. Pelvic magnetic resonance imaging (MRI) did not reveal a uterus or bilateral fallopian tubes, but nodules on both sides of the pelvic wall indicated cryptorchidism. The karyotype was 46,XY. Genetic testing identified a maternal-derived hemizygous variation c.2359C > T (p.Arg787*) in the AR gene. During abdominal exploration, dysplastic testicles and a dysplastic uterus were discovered. Histopathological analysis revealed the presence of fallopian tube-like structures adjacent to the testicles. The CAIS patient documented in this study exhibited concurrent MDRs, thus expanding the spectrum of clinical manifestations of AIS. A review of prior literature suggests that the incidence of CAIS combined with histologically MDRs is not uncommon. Consequently, the identification of MDRs in AIS cases may represent an integral aspect of clinical diagnosis for this condition.
Concomitant use of renin-angiotensin system inhibitors augments the efficacy of immune checkpoint inhibitors: a systematic review and meta-analysis
Authors: Yu Junjie , Meng Fangang , Sui Wenxia , Yu Junmin , Shen Jinhai
Published: June 2024

Background

The impact of renin-angiotensin system inhibitors (RASIs) on the outcome of hypertensive cancer patients undergoing immune checkpoint inhibitor (ICIs) therapy remains ambiguous. This investigation sought to elucidate the consequences of RASIs use on the prognosis for this specific patient group within the context of ICIs treatment, aspiring to provide a clearer basis for rational, evidence-driven choices in the clinical prescription of these medications.

Methods

A comprehensive search was conducted on PubMed, Embase, Web of Science, and the Cochrane Library for original studies published up to 6 August 2023. Studies published in English reporting hazard ratios (HRs) with 95% confidence intervals (CIs) for overall survival (OS) and/or progression-free survival (PFS) were included. All statistical analyses were executed utilizing R software (version 4.2.2).

Results

A total of 13 studies, encompassing approximately 12,595 patients, satisfied the inclusion criteria. Meta-analyses demonstrated a statistically significant association between the use of RASIs and a favorable outcome in OS (HR, 0.74; 95% CI, 0.62–0.88) and PFS (HR, 0.77; 95% CI, 0.62–0.96) among cancer patients receiving ICIs treatment.

Conclusion

This investigation provides compelling evidence supporting the beneficial prognostic impact of RASIs on cancer patients receiving ICIs. RASIs present a viable option as antihypertensive agents for cancer patients with hypertension undergoing ICIs treatment. Further exploration and validation through prospective studies are necessary to establish definitive guidelines for the use of RASIs in managing hypertensive cancer patients undergoing immunotherapy with ICIs.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42023454886.
Case report: EGFR-TKI rechallenge after osimertinib-induced interstitial lung disease: a case report and literature review
Authors: Gu Xiaofei , Zhong Yonghong , Huang Huaqiong
Published: June 2024

Background

Osimertinib, a third-generation tyrosine kinase inhibitor (TKI), has demonstrated significant efficacy in treating non-small cell lung cancer (NSCLC) patients with epidermal growth factor receptor (EGFR) mutations. However, EGFR-TKI-induced interstitial lung disease (ILD), a well-known adverse effect, can seriously affect the treatment outcome. There is currently no international consensus on the efficacy and safety of re-administration of EGFR-TKI after EGFR-TKI-induced ILD.

Case summary

We report a case of a 62-year-old male with stage IV lung adenocarcinoma and EGFR L858R mutation who was treated with osimertinib at a dose of 80 mg/day as first-line therapy. On the sixth day of treatment, the patient developed grade 4 ILD, chest tightness, shortness of breath, and paroxysmal dry cough. Arterial blood gas analysis indicated the presence of type I respiratory failure, while the chest CT scan revealed newly developed ground-glass opacities in both lungs and a considerable amount of pleural effusion on the left side. Subsequently, the patient was administered methylprednisolone for anti-inflammatory therapy, in conjunction with oxygen therapy, anti-infection treatment, and closed thoracic drainage, which resulted in a favourable recovery and discharge after 18 days. During this period, the patient adhered to third-generation EGFR-TKI oral targeted therapy. Nevertheless, within a week of discharge, the patient was readmitted due to the recurrence of chest tightness and shortness of breath. A chest CT scan indicated a recurrent ILD. Despite the administration of high-dose methylprednisolone for 9 days, the patient’s condition continued to deteriorate, ultimately resulting in death.

Conclusion

It is of the utmost importance to conduct a meticulous evaluation of the severity of osimertinib-induced ILD in order to ascertain the potential risks and benefits of EGFR-TKI rechallenge. Particularly, for patients with grade 4 ILD, firm drug discontinuation should be considered.
Precrop-treated soil influences wheat (Triticum aestivum L.) root system architecture and its response to drought
Authors: Cope Jonathan E. , Berckx Fede , Galinski Anna , Lentz Jonas , Nagel Kerstin A. , Fiorani Fabio , Weih Martin
Published: June 2024

Aims

Root system architecture (RSA) plays an important role in the plant’s ability to sustain yield under abiotic stresses such as drought. Preceding crops (precrops) can affect the yield of the proceeding crop, partially by affecting the RSA. This experiment aims to explore the interactions between precrop identity, crop genotype and drought at early growth stages.

Methods

Rhizotrons, sized 60 × 80 × 3.5 cm, were used to assess the early root growth of two winter wheat (Triticum aestivum L.) genotypes, using precrop-treated soil around the seedlings and differing water regimes. The rhizotrons were automatically imaged 3 times a week to track root development.

Results

Precrop-treated soil affected the RSA and changes caused by the reduced water treatment (RWT) were different depending on the precrop. Largest of these was the 36% reduction in root depth after wheat, but 44% after OSR. This indicates that effects caused by the precrop can be simulated, at least partially, by transferring precrop-treated soils to controlled environments. The genotypes had differential RSA and reacted differently to the RWT, with Julius maintaining an 8.8-13.1% deeper root system compared to Brons in the RWT. In addition, the combined environmental treatment affected the genotypes differently.

Conclusion

Our results could help explain discrepancies found from using precrops to enhance yield as they indicate differences in the preceding crop effect when experiencing drought stress. Further, these differences are affected by genotypic interactions, which can be used to select and adapt crop genotypes for specific crop rotations, depending on the year. Additionally, we have shown a viable method of stimulating a partial precrop effect at the seedling stage in a controlled greenhouse setting using field soil around the germinated seed.
Accountability and oversight in the Dutch intelligence and security domains in the digital age
Authors: Constantino Jorge , Wagner Ben
Published: June 2024

Accountability is considered a cornerstone of public administration and good governance. This study characterizes the relationship between the Dutch Intelligence and Secret Service (“AIVD”) and citizens (represented by parliament, courts, and oversight boards) as a complex actor-forum relationship. We utilize different accountability principles of public administration found in international and Dutch instruments and academic literature to propose workable principles of accountability for the AIVD. These proposed principles of accountability can be summarized as acting within duty, explainability, necessity, proportionality, reporting and record keeping, redress, and continuous independent oversight. Similarly, there are some conditions to support the workability of accountability principles. These conditions may be characterized as productive actor-forum relationships, cooperation, flexibility, value alignment, and learning and improving opportunities.
Exploring the relationship between mindfulness and burnout among preschool teachers: the role of dispositional equanimity and empathy
Authors: He Yuanqing , Wang Xin , Wang Xiaqing , Li Xiaowen
Published: June 2024

The objective of this study is to delve into the underlying mechanisms between mindfulness and burnout among preschool teachers. Employing a cross-sectional research design, this study surveyed 1,980 Chinese preschool teachers using the Mindful Attention Awareness Scale (MAAS), Preschool Teacher Dispositional Equanimity Questionnaire (PTDEQ), Empathy Scale (ME), and Maslach Burnout Inventory for Educators (MBI-ES). The results revealed a significant negative correlation between preschool teachers’ mindfulness and burnout. A mediation analysis demonstrated that dispositional equanimity served as a mediator between mindfulness and preschool teacher burnout. Furthermore, a moderation analysis indicated that empathy moderated the influence of dispositional equanimity on preschool teacher burnout. These findings suggest that mindfulness can enable preschool teachers to better cope with workplace challenges with a more peaceful mindset.
The experience of effort in ADHD: a scoping review
Authors: Wagner Danika , Mason Samantha G. , Eastwood John D.
Published: June 2024

Background

Mental effort plays a critical role in regulating cognition. However, the experience of mental effort may differ for individuals with Attention-Deficit/Hyperactivity Disorder (ADHD), a disorder for which sustained mental effort ‘avoidance’ or ‘dislike’ is a criterion in the DSM. We conducted a scoping review to characterize the literature on the experiences of effort in ADHD.

Methods

This systematic scoping review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) Extension for Scoping Reviews and Joanna Briggs Methodology. PsycINFO (OVID), PsycINFO (ProQuest) and PubMed were searched for studies published in English before February 14, 2023. Studies must have included an ADHD population or a measure of ADHD symptomatology, in addition to a self-report measure of the experience of effort or the use of an effort preference paradigm. Two researchers reviewed all abstracts, and one researcher reviewed full-text articles.

Results

Only 12 studies met the inclusion criteria. Several gaps and inconsistencies in the research were identified in terms of method, definitions of effort, measurements of ADHD, and sample characteristics. Moreover, the pattern of results on the experience of effort was mixed.

Conclusion

Despite its diagnostic and conceptual significance, the experience of mental effort in ADHD is not well studied. Critical gaps were identified in the existing literature. A three-facet conceptualization of effort is proposed–specifically, task-elicited effort, volitionally exerted effort, and the affect associated with engaging in effort – to guide future explorations of the experience of effort in ADHD.
Emotional processing is not enough: relations among resilience, emotional approach coping, and posttraumatic stress symptoms among combat veterans
Authors: Shorer Shai , Weinberg Michael , Cohen Lihi , Marom Doron , Cohen Miri
Published: June 2024

Combat soldiers are exposed to various potentially traumatic events and face high risk of developing military-related psychopathology, such as depression, posttraumatic stress and grief (PTSS). However, a strong body of research shows that resilience is the default in the aftermath of trauma and indeed, many veterans do not develop high symptomatic levels. To explicate this inconsistency, the current study examined the associations among PTSS, resilience, and patterns of emotional-approach coping. A sample of 595 male combat veterans filled out questionnaires on trauma exposure, PTSS, depressive symptoms, resilience, and emotional-approach coping. Their data were analyzed using structural equation modeling path analysis. Participants reported high exposure to potentially traumatic events during service. Mean scores were high for resilience and relatively low for PTSS and depressive symptoms; 13% had a clinical level of posttraumatic stress disorder. Structural equation modeling revealed that emotional-approach coping strategies mediated the relationship between resilience and PTSS. However, emotional expression was associated with lower PTSS levels, whereas emotional processing was associated with higher PTSS levels. These results suggest that although emotional-approach coping was related to higher resilience, emotional expression (an intrapersonal coping strategy) might have a more positive effect than self-oriented emotional coping strategies. Providing veterans with supportive opportunities and a wider repertoire of emotional coping skills might enhance their well-being, reduce postservice emotional distress while not harming veterans’ resilience levels.
Circulating GDF-15: a biomarker for metabolic dysregulation and aging in people living with HIV
Authors: Wang Ling , Zhao Juan , Schank Madison , Hill Addison C. , Banik Puja , Zhang Yi , Wu Xiao Y. , Lightner Janet W. , Ning Shunbin , El Gazzar Mohamed , Moorman Jonathan P. , Yao Zhi Q.
Published: June 2024

Despite effective control of HIV replication by antiretroviral therapy (ART), a significant number of people living with HIV (PLWH) fail to achieve complete immune reconstitution and thus are deemed immune non-responders (INRs). Compared with immune responders (IRs) who have restored their CD4 T cell numbers and functions, CD4 T cells from these INRs exhibit prominent mitochondrial dysfunction and premature aging, which play a major role in increasing the incidence of non-AIDS, non-communicable diseases (NCDs). To date, there are no reliable biomarkers that can be used to typify and manage PLWH, especially INRs with non-AIDS NCDs. Growth differential factor-15 (GDF-15) is a transforming growth factor-β (TGF-β) family member known to regulate several biological processes involved in cell aging and stress responses. Since PLWH exhibit premature aging and metabolic dysregulation, here we measured the plasma levels of GDF-15 by ELISA and metabolic proteins by proteomic array and correlated the results with clinical parameters in ART-controlled PLWH (including INRs and IRs) and healthy subjects (HS). We found that GDF-15 levels were significantly elevated in PLWH compared to HS. GDF-15 levels were positively correlated with age and negatively associated with body mass and LDL cholesterol levels in the study subjects. Also, elevated GDF-15 levels were correlated with differential dysregulation of multiple metabolic proteins in PLWH. These results suggest that GDF-15 protein may serve as a biomarker of metabolic dysregulation and aging, and this biomarker will be useful in clinical trials targeting aging and metabolic disorders in ART-treated PLWH.
Revised clusters of annotated unknown sounds in the Belgian part of the North sea
Authors: Calonge Arienne , Parcerisas Clea , Schall Elena , Debusschere Elisabeth
Published: June 2024

Acoustic signals, especially those of biological source, remain unexplored in the Belgian part of the North Sea (BPNS). The BPNS, although dominated by anthrophony (sounds from human activities), is expected to be acoustically diverse given the presence of biodiverse sandbanks, gravel beds and artificial hard structures. Under the framework of the LifeWatch Broadband Acoustic Network, sound data have been collected since the spring of 2020. These recordings, encompassing both biophony, geophony and anthrophony, have been listened to and annotated for unknown, acoustically salient sounds. To obtain the acoustic features of these annotations, we used two existing automatic feature extractions: the Animal Vocalization Encoder based on Self-Supervision (AVES) and a convolutional autoencoder network (CAE) retrained on the data from this study. An unsupervised density-based clustering algorithm (HDBSCAN) was applied to predict clusters. We coded a grid search function to reduce the dimensionality of the feature sets and to adjust the hyperparameters of HDBSCAN. We searched the hyperparameter space for the most optimized combination of parameter values based on two selected clustering evaluation measures: the homogeneity and the density-based clustering validation (DBCV) scores. Although both feature sets produced meaningful clusters, AVES feature sets resulted in more solid, homogeneous clusters with relatively lower intra-cluster distances, appearing to be more advantageous for the purpose and dataset of this study. The 26 final clusters we obtained were revised by a bioacoustics expert. We were able to name and describe 10 unique sounds, but only clusters named as ‘Jackhammer’ and ‘Tick’ can be interpreted as biological with certainty. Although unsupervised clustering is conventional in ecological research, we highlight its practical use in revising clusters of annotated unknown sounds. The revised clusters we detailed in this study already define a few groups of distinct and recurring sounds that could serve as a preliminary component of a valid annotated training dataset potentially feeding supervised machine learning and classifier models.
Optimizing the water-ecosystem-food nexus using nature-based solutions at the basin scale
Authors: Maragkaki Antonia , Koukianaki Evangelia A. , Lilli Maria A. , Efstathiou Dionissis , Nikolaidis Nikolaos P.
Published: June 2024

The water ecosystem food (WEF) nexus duly acknowledges the complex interdependencies among water, ecosystems, and food production, underscoring nature based solutions (NBS) as an efficacious strategy for navigating these interconnections. In this research, four different NBS (terraces, riparian forest, livestock management and agro ecological practices) were assessed in terms of their impact to WEF nexus. The Karst-SWAT and the one-dimensional integrated critical zone (1D-ICZ) models were used to simulate the impact of NBS on water quantity and quality as well as on soil ecosystem services of Koiliaris River Basin, which serves as an illustrative example of a basin that has experienced severe soil and biodiversity degradation. The Karst-SWAT model showed that a combination of NBS of terraces and riparian forest can reduce soil erosion and the sediment load by 97%. The 1D-ICZ model successfully simulated the soil-plant-water system and showed that agro ecological practices affect biomass production, carbon and nutrient sequestration, soil structure and geochemistry.
Constrains in the articulation between profession, family, and personal life: a case study of the Portuguese police (PSP)
Authors: Rocha Ana , Espírito Santo Paula , Ferreira Ana Paula
Published: June 2024

The dangers and stresses of a police officer’s career cause physical and psychological distress which in turn cause considerable challenges for the integration of professional, family, and personal life. This study focuses on the conditions that influence this articulation between the personal, family, and professional lives of Portuguese Police (PSP1) officers. A questionnaire survey was applied to 414 police officers from 11 divisions of COMETLIS.2 At the empirical level, several models were tested to assess the articulation between the professional, family, and personal lives of police officers. The results highlight the influence of having young children, elderly people or other dependents living in the family home, as well as the employee’s age, on the job’s interaction with professional life. At a professional level, the variables shift work, professional category, years of service and working away from home were identified by the officers as conditioning factors in the work-family-personal life balance. Sex differences in the articulation of professional, family, and personal life were also observed.
Factors affecting the adoption of high technology in vegetable production in Hanoi, Vietnam
Authors: Nhuong Bui Huy , Truong Dinh Duc
Published: June 2024

High-tech vegetable production is becoming a priority in agricultural development in Vietnam in the context of digital economic development. This study aims at identifying factors involving the adoption of high technology in vegetable production by local farmers in Hanoi. We used the theory of planned behavior and other farmers’ personal, social and economic factors to develop empirical model and hypotheses. Primary data was collected from a survey of 450 vegetable producers in Hanoi using cluster sampling method combined with random selection. Then, binary logit model was used to analyze the impact of influencing factors. Results showed that there were 7 factors having significant influences the decision to apply technology in vegetable production of farmers including attitude on high tech production, access to information, size of farm, member of extension organization, education level, access to credit and perceived behavior control, in which attitude variable was the most influential factor. Main management implications raised included enhancing access to technological information, providing demostration visits, giving more extension services, improving social inclusion and implementing hi- tech training for farmers in vegetable production.