bluuebunny/crossref_metadata_2025 huggingface dataset:
Mirror Therapy Combined With Neuromuscular Electrical Stimulation for Poststroke Lower Extremity Motor Function Recovery: A Systematic Review and Meta-Analysis
Authors: Oh Zhen-Han , Liu Chia-Hung , Hsu Chih-Wei , Liou Tsan-Hon , Escorpizo Reuben , Chen Hung-Chou
Published: January 2023

The combination of mirror therapy (MT) and neuromuscular electrical stimulation (NMES) has been devised as an intervention method in stroke rehabilitation; however, few studies have investigated its efficacy in lower extremity motor function recovery. In this systematic review and meta-analysis, we examined the effectiveness of combined MT and NMES therapy in improving poststroke walking speed, gait ability, and balance. Randomized controlled trials (RCTs) were selected from PubMed, Cochrane Library, EMBASE, and Scopus databases. In total, six RCTs which involving 181 participants were included. Our findings indicate that MT combined with NMES elicits greater improvement relative to control group in walking speed (SMD = 0.67, 95% confidence interval [CI]: 0.26–1.07, P = 0.001), Berg Balance Scale (SMD = 5.47; 95% CI: 1.22–9.72; P = 0.01), cadence (SMD = 10.35, 95% CI: 1.15–19.55, P = 0.03), step length (SMD = 6.03, 95% CI: 2.52–9.54, P = 0.0008), and stride length (SMD = 13.10, 95% CI: 3.20–22.99, P = 0.009) but not in modified Ashworth scale (SMD = − 0.35, 95% CI: −0.75 to 0.06, P = 0.09). Our findings suggest that MT combined with NMES may be a suitable supplemental intervention to conventional therapy in stroke survivors.
Potential contribution of prioritized spatial allocation of nature-based solutions to climate neutrality in major EU cities
Authors: Pan Haozhi , Page Jessica , Shi Rui , Cong Cong , Cai Zipan , Barthel Stephan , Thollander Patrik , Colding Johan , Kalantari Zahra
Published: January 2023

We assessed the mitigation potential of nature-based solutions (NbS) within commonly overlooked pathways, including human behavioral interventions and resource savings, in addition to the well-understood carbon sequestration area. We found that general NbS implementation in the residential, transport, and industrial sectors of European cities can reduce urban carbon emissions by up to 25%. Based on spatial patterns of carbon emissions and the local context of each city, we then prioritized spatial allocation of different types of NbS implementations within 54 major EU cities, in order to maximize the carbon emissions reduction potential. We found that prioritized NbS could reduce human activity-related carbon emissions by on average 17.4% for all cities, with 8.1%, 14.0%, and 9.6% reduction in the residential, industrial, and transport sector, respectively, while 5.6% of the remaining carbon emissions could be captured by carbon sequestration. Projections to 2030 showed that prioritized NbS implementations on all available land parcels in the RCP 1.9 scenario would reduce total carbon emissions by on average 62.5% (95% CI: 47.9–66.7%) compared with the baseline scenario, with NbS capturing 22.0% marginal emissions and sequestration capturing 13.3%. Some pioneering cities climate action are projected to be very close to achieve carbon neutrality by 2030 while 3 cities can realize the goal. For carbon neutrality, cities therefore need to co-integrate indirect (human behaviors and resource saving) and direct (sequestration) contributions of NbS into aggressive climate action plans.
The 5:2 diet is feasible and reduces risk factors for cardio-vascular disease in subjects with and without type 2 diabetes
Authors: Ekberg Neda Rajamand , Hellberg Anton , Sundqvist Michaela , Hirschberg Angelica Linden , Catrina Sergiu-Bogdan , Brismar Kerstin
Published: January 2023

The 5:2 diet, a type of intermittent fasting has recently gained popularity. The aim was to evaluate the feasibility and effects of the 5:2 diet for six months on weight, metabolic and hormonal risk factors for cardiovascular disease (CVD) in overweight subjects with and without type 2 diabetes (T2D). 97 (59 women) subjects, 35 with T2D and 62 without T2D (controls) followed the 5:2 diet (two days/week on a 500 (women) or 600 (men) kcal menu) for six months. Only 4/97 subjects discontinued. A weight loss of 4.2 (5.0–3.3) kg (4.94%) and 5.3 (6.4–4.2) kg (6.43%) was observed in controls and T2D, respectively. In both groups there were decreases in waist circumference, fat percentage, and blood pressure. Waist circumference decreased more in T2D compared to the controls. HbA1c improved in the T2D group. Metabolic control, lipids, adiponectin and leptin improved. Age-adjusted IGF-I decreased in the controls and IGF binding protein-1 increased in the T2D group suggesting improved hepatic insulin sensitivity in T2D. The 5:2 diet was feasible for 6 months and resulted in significant weight loss and improvement in metabolic and hormonal risk factors for CVD in overweight and obese subjects with and without T2D.
Effect of uniform strain on graphene surface plasmon excitations
Authors: Lemus C. , Cruz G Gonzalez de la , Oliva-Leyva M.
Published: January 2023

This paper reports a study of the reflectance of the optical sensors based on graphene under uniform strain. Assuming the graphene layer is surrounded by two different semi-infinite dielectric media, the generalized Fresnel coefficients are derived as a function of usual quantities (e.g., dielectric constants, incident angles and strain) and anisotropic optical conductivity. The strain not only changes the electronic band structure, but also can be employed to tune the electronic collective excitations (plasmons) and thus the optical reflectance of graphene monolayers. One of the most common techniques for plasmon excitation is the Kretschmann configuration. It is based on the observation of a sharp minimum in the reflection coefficient versus angle (or wavelength) curve. Because strain induces anisotropy in graphene optical conductivity the strain-dependent orientation plays an important role to manipulate the variation of graphene plasmon energy, which may be useful to synchronize graphene properties in plasmonic devices to enhance light-matter interactions.
The effect of water on the interaction between species in Deep-Eutectic Solvents Based on Choline Chloride and Fatty Acids: insights from molecular dynamics simulation
Authors: Pour Samaneh Barani , Sardroodi Jaber Jahanbin , Ebrahimzadeh Alireza Rastkar , pazuki gholam reza
Published: January 2023

DESs based on fatty acids are potential candidates for removing pollutants from aqueous environments. However, the structural properties must manifest the stability of DESs in the adjacent water. In this work, the structural stability of the binary mixtures of fatty acids (FAs) and choline chloride (Ch+Cl-) at different ratios was investigated in adjacent water using molecular dynamics simulations. The binary mixtures with the mole percent at 30 % of [Ch+Cl-] and 70 % of FAs have more stability than other ratios. We also observed that the interaction between the chloride anion and the hydroxyl group of the cation leads to the transition of HBA to the water-rich phase. These atomic sites have fundamental importance in the interaction between HBA and HBD.
A Multiple Features Fusion based Node Importance Measure for Rumor Control in Social Networks
Authors: Wang Yucui , Wang Jian , Huang Mengjie , Yu Guiming , Wang Zhangquan , Guo Shiyuan , Yang Jian
Published: January 2023

In the field of social networks rumor control, finding the important nodes with the greatest propagating influence is of great practical importance to effectively control the propagation of rumors. In order to solve the problem that the network coupling information and information transfer mechanism in the existing node importance metric affects the accuracy of the metric, we propose a measure of node importance based on Multiple Feature Fusion (MFF). The method uses an improved Dempster-Shafer evidence theory to fuse the centrality, transmissibility, and prestige measurement of nodes and rank the importance of nodes based on the fusion results. The proposed method was first evaluated against similar methods on six real networks in terms of robustness and vulnerability, as well as in terms of SIR propagation characteristics. Then, we simulated the changes in the number of users who believed the rumor after the rumor was propagated in three cases: before the control, after suppress rumors by random nodes, and after suppress rumors by important nodes. The experimental results show that the proposed method is more accurate for node importance measurement and more effective in rumor control.
Spatial and Seasonal Difference in Physio-Chemical Features of Sediment Cores from Selected Creeks in Niger Delta, Nigeria
Authors: Eguvbe Peter Mamuro
Published: January 2023

Live DNA: 234.17895*
The researcher studied the spatial and seasonal changes in pH, electric conduction and total organic carbon in sediment cores of Ubeji, Ifie, and Egbokodo Creeks in the Niger Delta, Nigeria. The researcher took a total of 222 sediment core samples during wet and dry seasons. The researcher dried, sieved, and analyzed the samples using standard calculus methods. The pH of the sediment ranged from 2.25 to 4.80 (wet season), and 2.12 to 4.42 (dry season). Which implies that the sediment cores are acidic in both wet and dry season. The Electrical conduction of the core sediment ranged between 620 and 5,620 µS/cm (wet season) and between 800 and 5900 µS/cm (dry season). Total Organic Carbon of all nine cores decreased slightly with increase of sediment core depth. This study provided data on the physio-chemical properties of sediment cores, which may be useful for control actions, surrounding quality governance, and eco forensic studies.
What types of information do pharmacists include in comprehensive medication management review reports? A qualitative content analysis
Authors: Al-Diery Tarik , Freeman Hollie , Page Amy Theresa , Cross Amanda J , Hawthorne Deborah , Lee Kenneth
Published: January 2023

Introduction

Comprehensive medication management reviews are an established intervention to identify medication-related problems, such as the prescribing of potentially inappropriate medications, and under- and over-prescribing. However, the types of information included in written reports of comprehensive medication management reviews, beyond types of medication-related problems, are unknown. This study aimed to explore the types of information Australian pharmacists include in their written reports following comprehensive medication management reviews.

Methods

Australian consultant pharmacists were invited to upload their 10 most recent written reports of their domiciliary-based comprehensive medication management reviews. A random selection of the reports, stratified by each pharmacist, were included for qualitative content analysis.

Results

Seventy-two de-identified reports from eight consultant pharmacists were included for analysis. From the evaluated reports, four major categories of information were identified: 1) consumer details such as date of interview (n = 72, 100%) and medicine history (n = 70, 97%); 2) pharmacist assessment including assessment of the consumer (n = 70, 97%), medicines management (n = 68, 94%) and medicine-related issues (n = 60, 83%); 3) pharmacist recommendations, specifically pharmacological recommendations (n = 67, 93%); and 4) consumer-centred experiences such as perspective on medicines (n = 56, 78%). Reporting of consumer-centred experiences appeared most variably in the included reports, including consumer concerns (n = 38, 53%), willingness for change (n = 27, 38%), consumer preferences (n = 13, 18%), and consumer goals (n = 7, 10%).

Conclusion

Pharmacists within our study included a wide variety of information in their comprehensive medication management review reports. Aside from medication-related problems, pharmacists commonly provided a holistic assessment of the consumers they care for. However, despite the breadth of information included in a comprehensive medication management review report, our study suggests that there is wide variability across reports, which has the potential to impact consistent service delivery.
Analysis for MHD Micro Polar Fluid Over a Melting Stretching Surface with Slip Effect
Authors: Sharma Surbhi , Dadheech Amit , Parmar Amit , Arora Jyoti , Al-Mdal Qasem Al-Mdallal
Published: January 2023

Objective of present analysis is to represent the phenomenon of Heat –mass transfer on MHD micro polar fluids caused by permeable and continuously stretching sheet along with slip impacts fostered in a porous medium. Consequently, the equation of energy includes the term of non-uniform heat source/sink. The equation regarding to species concentration in cooperates the terms indicating order of chemical reaction to characterize the chemically reactive species. The application software MATLAB with governing syntax of bvp4c technique are employed to reduce equations of momentum, micro-rations, heat and concentration into suitable required simplifications to derive necessary arithmetic manipulations of available non-linear equations. Various dimensionless parameters are portrayed in the available graphs with essential consequences. Analysis discovered that micro-polar fluid improves velocity and temperature profile while it suppresses micro-rations profile. The acquired deductions verify remarkable correspondence with already reported in an open literature.
Wild plants for food in the Andes
Authors: Alva-Alvarado Indira Margaret , Torre-Cuadros María de los Ángeles La , Carro Roberto Ugás
Published: January 2023

Background
This ethnobotanical research was part of the Project "Ecological and Socioeconomic Intensification of the Small Andean Agriculture'' (AGROECO). The aim is to systematize traditional knowledge of wild food plants in the Andean community of Janac Chuquibamba (Cusco-Peru), to contribute to family food safety. The objectives of this paper are to identify the wild food plants used in this community and describe the local perceptions, use, seasonality, management, and commercialization of wild food plants in such place.
Methods
Data was collected through participatory observation, free listing, semi-structured interviews, focus groups, and walking in the crop fields.
Results
A total of 48 wild edible plants with local names and taxonomic classifications, belonging to 27 botanical families were reported. In addition, the farmers mentioned, within this category, 7 species from other kingdoms: 4 fungi, 1 algae, and 2 lichens. In the diet of the high Andean community of Janac Chuquibamba, a wide range of wild plant species is still included, through different preparations in the local cuisine. 91.7% of wild food plants have other uses in addition to food, and 77% of these plants are medicinal. There are more resources available in the wet season. The wild edible plants have different levels of management such as simple harvesting, tolerance, promoting, protection, transplanting, sowing, and planting. The agricultors reported selling 26 species of wild food plants in the nearby markets of Lamay, Calca, Coya, Pisac, Urubamba, and Chincheros, and even in Cusco.
Conclusions
The knowledge about these plants, as their use and management should be thoroughly documented, as well as promoted among local people and the general population, in order to contribute to the security and food sovereignty of these communities and society in general.
Psychological symptoms and inadequate mental health literacy potentially increase the risks of suicidal behaviors in Chinese middle school students
Authors: Chen Jin , Hu Jie , Yang Rong , Li Danlin , Zhang Zhixian , Jia Liyuan , Wan Yuhui , Fang Jun , Zhang Shichen
Published: January 2023

Background
Suicide is a major public health problem as the second leading cause of death for adolescent. Inadequate mental health literacy (MHL) and psychological symptoms are risk factors of suicidal behaviors. But so far, no study has examined the interactive role of MHL and psychological symptoms in suicidal behaviors. In this context, the purpose of this study was to examine the independent and interactive association of MHL and self-reported psychological symptoms with suicidal behaviors in Chinese middle school students.
Methods
A total of 16 853 middle school students were recruited from October 2020 to June 2021. Psychological symptoms, MHL and suicidal behaviors were measured by self-reported validated instruments. Logistic regression models were conducted to examine relations between them.
Results
The prevalence of suicidal ideation, suicidal plan and suicidal attempt were 31.3%, 16.3% and 7.5%, respectively, Inadequate MHL was significantly associated with suicidal ideation, suicidal plan and suicidal attempt [OR (95% CI) was 1.100 (1.018–1.189), 1.246 (1.125–1.380), 1.739 (1.488–2.033)]. Psychological symptoms were significantly related with suicidal ideation, suicidal plan and suicidal attempt [OR (95%CI) was 4.749 (4.268–5.284), 5.463 (5.069–6.283), 5.970 (5.236–6.806)]. Furthermore, psychological symptoms and inadequate MHL were interactively associated with increased risks of suicidal behaviors.
Conclusions
These finding indicated that Chinese middle school students having psychological symptoms and inadequate MHL are at risk of exhibiting suicidal behaviors. Enhancing MHL and attenuating the severity of psychological symptoms should be considered and involved in the development of early intervention programs for suicide.
Anakinra as first-line therapy for systemic onset juvenile idiopathic arthritis: a retrospective study in a French Single-center
Authors: Ravalet Adeline , Granel Jerome , Daghistani Aseel , Clet Johanna , Richer Olivier , Molimard Julie , Lerouge-Bailhache Marion , Pillet Pascal
Published: January 2023

Background
Systemic juvenile idiopathic arthritis (sJIA) is a serious disease with significant morbimortality. Management of sJIA has improved dramatically over the last decade, especially with IL-1 receptor antagonists (IL-1Ra), but treatment remains heterogeneous. In this study, we described the evolution of patients with sJIA treated with IL1Ra, mainly as first-line therapy.
Methods
In this single-center retrospective observational study, we included all patients with sJIA treated with anakinra (ANK) between 2006 and 2020.
Results
Twenty-four patients received ANK after a median delay of 28 days (range 0–2070 days) after the diagnosis of sJIA. The median follow-up time was 2.37 years (range 0.49–10.75 years). Only 7/24 (29%) patients received corticosteroids. Inactive disease was observed in 18/24 (75%) patients at 3 months. Remission was observed in 19/23 (83%) patients at 1 year. At last available follow-up, 16/24 (67%) patients were in remission attributable to ANK and treatment-free remission was observed in 12/24 (50%) patients. Remission attributable to ANK was obtained in 12/15 (80%) patients treated with ANK within the first 3 months of the disease versus 4/9 (44%) patients treated after 3 months (p = 0.1). An initial White Blood Count ≥ 20 G/L was associated with remission attributable to ANK (p = 0.03). The only death occurred in a patient with a long-standing disease before ANK initiation and was attributable to the disease. Adverse events observed included ear, nose and throat infections and one transient liver cytolysis.
Conclusions
ANK in sJIA allows rapid remission with few adverse events. Their early use, as a firts-line therapy would allow rapid remission while avoiding the side effects of corticosteroids.
Trial registration:
retrospectively registered.
Estimating health risk assessment due to pesticide exposure through dietary intake in biomonitoring of metabolites in Indian children's public health
Authors: sinha sukesh
Published: January 2023

Pesticide residues are one of the most dangerous things that can affect the safety and quality of agro-food. We assessed study participants' long-term dietary exposure to 17 OPs and looked at how conventional food consumption and OP urinary metabolites in young children's and adolescents' urine samples impacted this exposure. We investigate the OP pesticide and metabolite concentrations in the study participants' diet and urine for the extraction and quantitative analyses of pesticides by using the QuEChERS method & followed by the LC-MS/MS. We gathered the food intake data of around 6–15-year-old (N = 905) child volunteers based on parent interviews and food questionnaires from the study proforma and also collected their urine samples. The health risk assessments through EDI, ADI, and chronic or long-term health (HQ) were estimated. Our results suggest that 17 OP pesticides were found in food samples. Among 905 study participants based on diet, there was a significant (p < 0.000) difference in the consumption of fruits & vegetables between the males and females, sex-wise, and the adolescents and child, age-wise. We investigated the DAP levels in urine samples, and there was a significant (p < 0.05) difference in the concentration of DAPs levels between the child female, & HIG groups, which showed more DAP levels than the child male, LIG, & MIG groups. The analysis of EDI and health quotient (HQ) values of pesticides on study participants showed that pesticides such as dichlorvos, monocrotophos, dimethoate, quinalphos, triazophos, and ethion were significantly associated with the health risks of children through their diets. The findings indicate that there is more risk associated with fruits, fruit juices, and vegetables compared to plain foods, but future total exposure to these toxins from a variety of foods warrants extra vigilance.
Increased expression of TRPV1 in the central nucleus of the amygdala is involved in orthodontic pain during experimental tooth movement in rats
Authors: Wang Rui , Fang Dan , Lu Shuyun , Jia Yunfei , Guo Yining , Liu Xianya , Qiao Hu
Published: January 2023

Pain is one of the most common adverse reactions during orthodontic treatment, which troubles patients a lot. Transient receptor potential vanilloid 1 (TRPV1) plays a crucial role in pain transmission and is expressed in the peripheral nervous system, but there is a paucity of literature on the roles of TRPV1 in the central nervous system. The central amygdala (CeA) integrates multiple sensory signals including nociceptive sensory information. However, how the involvement of TRPV1 in the CeA in orthodontic pain has not been investigated. To explore this, we constructed an experimental tooth movement (ETM) model using precision springs and evaluated pain behaviour based on face-grooming and the rat grimace scale (RGS). TRPV1 expression in the CeA was evaluated using immunofluorescence and western blotting. Face-grooming and RGS score peaked on day 1 then decreased gradually to baseline levels on day 7. Immunofluorescence and western blotting analysis revealed that TRPV1 expression in the CeA increased after ETM. Furthermore, changes in TRPV1 expression in the CeA were positively associated with RGS behaviour. Our findings suggest that TRPV1 in the CeA is modulated by ETM and is involved in tooth-movement pain, providing a new understanding of central regulation on orthodontic pain.
10-Year Multicentre Experience of Penile Fracture Repair Outcomes
Authors: Yii Matthew , Gan Chrisdan , Qin Kirby , Bolton Damien , Blecher Gideon
Published: January 2023

Penile fractures are the traumatic rupture of the tunica albuginea encasing the corpora cavernosum. We reviewed long-term sexual and urinary outcomes following penile fracture repair. We performed a multicentre retrospective analysis with data from three metropolitan Australian hospital services over a 10-year period. Operative reports were reviewed and patients with intraoperatively confirmed fractures were included. Patients were contacted to complete a survey, composed of validated questionnaires including the International Index of Erectile Function-5 (IIEF-5) and International Prostate Symptom Score (IPSS). We analysed perioperative data against questionnaire responses. 55 patients were identified with penile fractures. 21 patients completed questionnaire follow-up. The median age was 44.8 years; the median time to follow-up was 4.0 years; 95.5% (20) of these cases occurred during sexual intercourse. The median IIEF-5 was 23 (no erectile dysfunction) and median IPSS was 5 (mildly symptomatic). Seven patients (33.3%) suffered bilateral cavernosal injury and eight (38.1%) suffered urethral injury. No significant relationships were found between injury factors and erectile or urinary function. We found promising outcomes for long-term erectile and urinary function. Study limitations include sample size and subjective outcome measurements. To completely define the relationship between injury factors and outcomes, larger cohorts will need to be analysed.
Comparison of penile doppler ultrasound versus Erection Hardness Score during intracavernous injection test in predicting refractory erectile dysfunction
Authors: Silva Alberto Costa , Martins-Silva Carlos , Morgado Afonso
Published: January 2023

Our group has shown that intracavernous injection test (ICI) have a better accuracy in predicting short-term response to therapy with sildenafil citrate versus penile doppler ultrasound (PDU). Our objective was to compare the prognostic value of PDU parameters with erection rigidity assessed by the Erection Hardness Score (EHS) during ICI in predicting refractory ED after 5-years. Patients referred for ED were evaluated and had a PDU with, at least, 15 ug of intracavernous alprostadil. Refractory ED was defined as having a penile prosthesis implanted, failed non-surgical treatments but refused penile prosthesis (PP) implantation or discontinuation of non-surgical treatments due to loss of efficacy. Receiver operating characteristic curves were drawn and the area under the curve (AUC) was calculated. At 5 years, 69 men were still in follow-up with a mean age of 58.47 years and 13 (18.8%) were classified as having refractory ED. The AUC for EHS, peak systolic velocity, end diastolic flow and resistive index to discriminate refractory ED were 0.820, 0.613, 0.730 and 0.714, respectively. Our data suggest that an abnormal EHS during an ICI could be non-inferior than an abnormal PDU in predicting response to non-surgical treatments and that will need a PP in long-term.
ERAMER: A Novel In silico Tool for Prediction of ERAP1 Enzyme Trimming
Authors: Al-okaily Anas , Khashabeh Razan Abu , Alsmadi Osama , Ahmad Yazan , Sultan Iyad , Mandoiu Ion , Tbakhi Abdelghani , Srivast Pramod
Published: January 2023

Somatic mutations in tumors can generate neoantigens that are presented on the cell surface through MHC molecules. As these mutations are expected to be unique to each tumor and as these generated (and presented) neoantigens play a significant role in mediating immune response against the tumor through tumor-specific T-cells, personalized cancer immunotherapy for each tumor can be designed, formed, and effective in curing cancer.
MHC class I pathway consists of four main steps: proteasomal cleavage in the cytosol in which proteins are cleaved into smaller peptides, precursors are then transported into the endoplasmic reticulum by the transporter associated with antigen processing (TAP), next further processing (trimming) at the N-terminal region is undertaken by an ER resident aminopeptidases 1 (ERAP1) enzyme to generate optimal lengths (8-10 amino acids) to produce a stable MHCI: peptide association, and finally the trimmed precursors are loaded into MHCI molecule to be transited via the Golgi apparatus to the cell surface for presentation to the cellualar immune system.
Several studies reported specificities related to the ERAP1 trimming process and yet there is no in silico tool for the prediction of the trimming process of the ERAP1 enzyme. In this paper, we provide and implement a prediction model for the trimming process of the ERAP1 enzyme.
The Effect of Rope Jumping Training on The Dynamic Balance Ability and Hitting Stability among Adolescent Tennis Players: A Systematic Analysis of Randomized Controlled Trials
Authors: Shi Zuozheng , Xuan Shu , Deng Yue , Chen long , Xu Binglong , Lin Bing
Published: January 2023

Background
This paper aims at discussing the effect of 12-week rope jumping training session on the performance of dynamic balance ability and hitting stability among young tennis players.
Methods
Sixteen young tennis players were recruited and randomly distributed into an experimental group and a control group to go through training for twelve weeks at a pace of three times a week. The experimental group went through rope jumping and special preparation activity (RJ + SPA). In contrast, the control group merely went through special preparation activity (SPA). Researchers ensured that the training content and intensity were essentially the same. The Biodex Balance System (BBS) was applied to examine the participants' dynamic balance ability, including the limits of stability(LOS) and the single-leg stability testing(SLST).Furthermore, International Tennis Number (ITN) was employed to test the stability overall.
Results
1) Based on the test results about the LOS the experiment participants' overall direction control (ODC) in the experimental group were significantly improved (p < 0.05). the control group overall direction control was not significantly improved (P > 0.05). In the experimental group's SLST, the overall stability index(OSI), the right of medial and lateral stability index (MLSI-R) increased significantly (P < 0.01). The anterior and posterior stability index (APSI) increased but not significantly (P > 0.05), while in the control group, only the right of medial and lateral stability index (MLSI-R) increased significantly (P < 0.05). The experimental group's overall stability index was significantly higher than that of the control group (P < 0.05).2) The stability of forehand and backhand hitting depth and accuracy was significantly improved in the experimental group (P < 0.05), while the figures in the control group were not significantly improved (P > 0.05). The experimental group's hitting stability performance was better than that in the control group as well (P < 0.05).
Conclusion
After 12-week experiment on rope jumping training sessions, participants dramatically improved their dynamic balance ability and hit stability. Furthermore, the effect of rope jumping training combined with designated technical and tactical training acquired better results than that of simple special technical and tactical training. It is suggested that the rope jumping training content of the corresponding load should be appropriately arranged in the youth tennis training course for better outcomes.
Footpad dermatitis, hock burns and condemnation rates at slaughterhouse as on-farm welfare indicators
Authors: Saraiva S. , Santos S. , Simões J. , Saraiva C.
Published: January 2023

Background: Data collected during meat inspection in slaughterhouses are important for monitoring and surveillance of the welfare and health of broilers.The objective of this study was to assess the health and welfare of 70 commercial broilers flocks in three different slaughterhouses based on: emaciation, dirty feathers (DF), footpad dermatitis (FPD), hock burn (HB), breast burn (BB), breast blister, breast ulcer, scratches, ascites, septicemia/abnormal color, cellulitis, extensive traumatisms, dead on arrival (DoA) and condemnation rate. A 3-point scale (0, 1 or 2) was used to classify DF, FPD and HB measures; a 2-point scale (present or absent) was used to classify BB, breast blisters, breast ulcer and scratches measures and the causes of total condemnation (ascites, septicemia/abnormal color, cellulitis, extensive traumatisms and DoA) were registered and presented in percentages. Eleven pavilions that obtained the worst results for these indicators were audited.
Results: Two flocks presented condemnations rates higher than 4% and 11 flocks presented DoA rates higher than 0.5%. Twenty-one flocks achieved the warning (grade 1) for FPD and 14 the alarm for FPD (grade 2). Extensive trauma was observed in 0.01% of the slaughtered animals and none flock reached the threshold of 2%. Breast blister, breast ulcer and scratches lesions were not observed in the studied flocks. The associations obtained for FPD2, HB2 and BB1, as well as for emaciation, HB0 and BB0 indicate simultaneous occurrences. FPD, HB, DoA and condemnation rates were the main indicators in the detection of farms with poor welfare conditions.
Conclusions: FPD, HB, DoA and condemnation rates should be systematically included in welfare monitoring of broilers. Audits on farms suggested that improvements in environmental and strutural conditions such as house temperature, insulation, ventilation, light program, air quality and reduce stocking densities are key factors to improve animal welfare on farms.
Dual-effect recombinant baculovirus mediated RNA interference with V-ATPase of fall armyworm, Spodoptera frugiperda
Authors: Bai Shanshan , Jin Duo , Jiang Yonggan , Chen Fenghua , Cheng Weining , Qi Zhijun
Published: January 2023

The fall armyworm (FAW, Spodoptera frugiperda), has rapidly spread from American continent to many other areas and caused serious damage to local agriculture. In order to explore a more environment-friendly and effective control strategy against this pest, we developed a dual-effect RNAi system based on baculoviruses infection and gene silencing. V-ATPase subunits A and B of FAW (SfvATPaseA and SfvATPaseB) were cloned for the construction of recombinant baculoviruses (rBviruses) of Bacmid-dsSfvATPaseA and Bacmid-dsSfvATPaseB with the capacity of producing small interfering RNA. The efficacy of baculovirus-mediated RNAi was evaluated by injection delivery. The results showed that the relative expression levels of SfvATPaseA and SfvATPaseB were significantly suppressed by constructed rBviruses with reduction rates of 73.85% and 69.82%. The larval inhibition rates of the body weight and length resulting from RNAi were more than 82% and 40%, respectively. More importantly, these two rBviruses greatly accelerated the larval death of FAW with a total mortality using two days less than that caused by bacmid control. After ten serial passages, both rBviruses exhibited excellent genetic stabilities. These results suggest that the creation of dual-effect biopesticides with both activities of viral infection and gene silencing is feasible and prospective.
Role of autophagy in the regulation of respiratory inflammation caused by zinc oxide nanoparticles
Authors: Zhang Hao , Cai Li , Su Cheng , Huang Xibao , Tong Yeqing
Published: January 2023

Objective: To study whether zinc oxide nano-particles (ZnO-NPs) can induce cellular autophagy in neutrophils, and whether cellular autophagy is capable of regulating the degree of inflammatory response induced by ZnO-NPs, as well as to lay a basis for research relating to the toxicity of ZnO-NPs on the respiratory system.
Methods: Peripheral blood neutrophils of rats were stimulated with different concentration gradients of ZnO-NPs (including 0, 5, 10, 15, 20 mg/L). The expression levels of cellular autophagy-associated protein LC3B were examined using immunoblotting (WB) method. The expression levels of inflammatory factor IL-8 in cell supernatants were examined through enzyme-linked immunoreagent adsorption assay (ELISA). The expression levels of cellular autophagy-associated proteins LC3B and P62 and inflammatory factors (IL-6, IL-8, IL-1β) were examined through stimulation with zinc oxide nanoparticles (20 mg/L) for 24 h after neutrophils were pretreated with autophagy agonist (RAPA) and autophagy inhibitor (3-MA).
Results: IL-8 and LC3B expression levels were up-regulated after the stimulation of neutrophils with ZnO-NPs, the pretreatment of cells with autophagy agonist (RAPA) up-regulated the expression of ZnO-NPs-induced IL-8, IL-6, and IL-1β, and the pretreatment of cells with autophagy inhibitor 3-MA down-regulated the expression of ZnO-NPs-induced IL-8, IL-6, and IL 1β.
Conclusion: ZnO-NPs are capable of inducing neutrophil autophagy, and cellular autophagy can regulate ZnO-NPs-induced neutrophil inflammatory response.
bHLH transcription factors Hes1, Ascl1 and Oligo2 exhibit different expression patterns in the process of physiological electric fields-induced neuronal differentiation
Authors: Li Zhe , Li Hai , Zhou Jiaying , Yu Xiyao , Dong Zhiyong , Meng Xiaoting
Published: January 2023

Background
Recent studies have shown that the expression of bHLH transcription factors Hes1, Ascl1, and Oligo2 has an oscillating balance in neural stem cells (NSCs) to maintain their self-proliferation and multidirectional differentiation potential. This balance can be disrupted by exogenous stimulation. Our previous work has identified that electrical stimulation could induce neuronal differentiation of mouse NSCs.
Methods
To further evaluate if physiological electric fields (EFs)-induced neuronal differentiation is related to the expression patterns of bHLH transcription factors Hes1, Ascl1, and Oligo2, mouse embryonic brain NSCs were used to investigate the expression changes of Ascl1, Hes1 and Oligo2 in mRNA and protein levels during EF-induced neuronal differentiation.
Results
Our results showed that NSCs expressed high level of Hes1, while expression of Ascl1 and Oligo2 stayed at very low levels. When NSCs exited proliferation, the expression of Hes1 in differentiated cells began to decrease and oscillated at the low expression level. Oligo2 showed irregular changes in low expression level. EF-stimulation significantly increased the expression of Ascl1 at mRNA and protein levels accompanied by an increased percentage of neuronal differentiation. What’s more, this tendency was increased with the extension of EF-stimulation time and reached the peak at 24h of EF-treatment.
Conclusions
We conclude here, EF-stimulation directed neuronal differentiation of NSCs by promoting the continuous accumulation of Ascl1 expression and decreasing the expression of Hes1.
Leveraging of Climate Fintech Model in India: Scoping through a Qualitative Approach using a Sentiments Analysis
Authors: G Sreelekshmi , V Biju A
Published: January 2023

Climate crisis has become a crucial concern for humankind as extremes in climate have grown manifold, mainly originating from anthropogenic activities. Growth and development are indispensable but worthless if our survival is under threat. Therefore, it demands prompt action from all stakeholders. However, colossal requirements of funds for climate sustainability remain a principal concern. India is one of the most active fintech markets in the world using domestic technologies like Unified Payment Interface (UPI), showing a fantastic growth trajectory in terms of international adoptions. With the boom of fintech solutions in India, the possibility of a novel fintech model with a climate action motive, known as climate fintech or green fintech, garnered entrepreneurial attention. Therefore, we intend to explore the concept of climate fintech and understand the state of the art using an inductive qualitative approach. With the help of the insights drawn from a systematic review of existing studies, a case study was conducted, followed by sentiment analysis, to reaffirm the results. Our findings suggest considerable scope for the climate fintech model to be beneficial for attaining the country’s climate targets. Our research anticipates that the area and climate fintech applications will advance in future.
Kinetic Trapping of 2,4,6-Tris(4-pyridyl)benzene and ZnI 2 into M 12 L 8 poly-[n]-catenanes using unconventional self-assembling processes
Authors: Rujas Javier Marti , Famulari Antonino
Published: January 2023

Here we show that in a supramolecular system with more than 20 building blocks forming the large icosahedral M12L8 metal-organic cages (MOCs), using the instant synthesis method it is possible to kinetically trap, and control the formation of interlocking M12L8 nanocages, giving the rare M12L8 TPB-ZnI2 poly-[n]-catenane. The catenanes are obtained in one-pot reaction, selectively as amorphous (a1) or crystalline states as demonstrated by powder X-ray diffraction (powder XRD), thermogravimetrical (TG) analysis and 1H NMR. The 300 K M12L8 poly-[n]-catenane single crystal X-ray diffraction (SC-XRD) structure including nitrobenzene (1), indicates a strong guest binding with the large M12L8 cage (i.e., internal volume ca. 2700 Å3) allowing its structural resolution. Conversely, slow self-assembly (5 days) leads a mixture of the M12L8 poly-[n]-catenane and a new TPB-ZnI2 (2) coordination polymer (i.e., thermodynamic product) as revealed by SC-XRD. The neat grinding solid-state synthesis also yields the amorphous M12L8 poly-[n]-catenane (a2), but not coordination polymers, selectively in 15 mins. The dynamic behavior of the M12L8 poly-[n]-catenanes demonstrated by the amorphous-to-crystalline transformation upon the uptake of ortho-, meta- and para-xylenes, shows the potential of M12L8 poly-[n]-catenanes as functional materials in molecular separation. Finally, combining SC-XRD of 1 and DFT calculations specific for the solid-state, the role of the guests in the stability of the 1D chains of M12L8 nanocages are reported. Energy interactions such as interaction energies (E), the lattice energies (E*), the host-guest energies (Ehost-guest) and the guest-guest energies (Eguest-guest) have been analysed considering the X-ray structure with and without nitrobenzene guest. Not only the synthetic control achieved in the synthesis of the M12L8 MOCs, but also their dynamic behavior either in the crystalline or amorphous phase, are sufficient to raise the scientific interest in areas ranging from fundamental to the applied sides of chemistry and material sciences.
Laboratory risk factors for coexistent primary biliary cholangitis in patients with Sjögren’s syndrome: a retrospective study
Authors: Gao Xuan , Xiao Guangzhi , Yang Fengfan , Dou Rongrong , Xue Miao , Zhang Yingying , Zheng Zhaohui , Ding Jin
Published: January 2023

Background
There is limited research on the laboratory characteristics of coexistent primary biliary cholangitis (PBC) and Sjögren's syndrome (SS). This study aimed to investigate the laboratory risk factors for the coexistence of PBC in patients with SS.
Methods
Eighty-two patients with coexistent SS and PBC (median age 52.50 years) and 82 age- and sex-matched SS controls were retrospectively enrolled between July 2015 and July 2021. The clinical and laboratory characteristics of the two groups were compared. Laboratory risk factors for the coexistence of PBC in patients with SS were analyzed using logistic regression analysis.
Results
Both groups had a similar prevalence of hypertension, diabetes, thyroid disease, and interstitial lung disease. Compared with the SS group, patients in the SS + PBC group had higher levels of liver enzymes, immunoglobulins M (IgM), G2, and G3 (P < 0.05). The percentage of patients with an antinuclear antibody (ANA) titer > 1:10000 in the SS + PBC group was 56.1%, higher than that in the SS group (19.5%, P < 0.05). In addition, cytoplasmic, centromeric, and nuclear membranous patterns of ANA and positive anti-centromere antibody (ACA) were observed more frequently in the SS + PBC group (P < 0.05). Logistic regression analysis showed that elevated IgM levels, high ANA titer, cytoplasmic pattern, and ACA were independent risk factors for PBC coexistence in SS.
Conclusion
In addition to established risk factors, elevated IgM levels, positive ACA, and high ANA titer with cytoplasmic pattern also provide clues to clinicians for the early screening and diagnosis of PBC in patients with SS.
Towards Standards for Registering and Documenting Heritage Villages in Egypt
Authors: Abdo Ahmed Mohamed , Hussein Omar Ibrahim , Hassanein Emad Mohamed , Mohamed Abdelaziz Farouk
Published: January 2023

There are still many villages that contain other undiscovered heritage components in addition to their historical heritage that expresses a different status and character from other environments, even though many of the heritage areas and buildings owned by the Egyptian state are World Heritage sites. Failure to adapt to local, regional, and global concepts and concepts as well as a lack of strength in advocating for the necessity of developing these villages would result in many cases of their loss rather than their benefit. Therefore, it is necessary to keep track of and record these towns in order to prioritize them in a bank according to the national goal that the state wants to progress the Egyptian village. The research paper aims to develop requirements and standards for monitoring and documenting Egyptian heritage villages, documenting, and registering them in accordance with the global and regional concept, which expands the opportunities for investment and credits for these villages and diversifies the Egyptian state's economic resources. The study uses analytical, descriptive, and inductive methodologies to reach its research objectives. The study is divided into three parts. The first part examines the characteristics of heritage villages and their distinctive elements at the global, regional, and local levels. The second part explores the standards and terms of reference for designating villages as historic at the global, regional, and local levels along with their historic components. In the third part of this study "The villages of Luxor Governorate," the heritage components were field monitored in order to assess the conditions and local reference standards for monitoring and recording the heritage villages in Egypt. Additionally, a group of experts and specialists were questioned about the standards that are not mentioned in the law and the likelihood to it is necessary to include them. Additionally, standing on the list of standards that serve as a tool for identifying and cataloging legacy villages with heritage components at the national, international, and regional levels in Egypt. The villages of the case study "Villages of Luxor Governorate" were then recorded, documented, and classified in order to determine the level of categorization for each village. This process produced a list of villages in Luxor Governorate, Egypt that had heritage components.