bluuebunny/crossref_metadata_2025 huggingface dataset:
Specifics of Physical Training of Future Border Guard Officers of Ukraine: Comparative Analysis
Authors: Tushko Klavdiia , Dyiak Vadym , Farion Oleh , Lysak Pavlo , Sych Ruslan , Demskyi Valentyn , Trifanina Liubov , Synyshyn Mykhailo
Published: March 2023

The article highlights the range of problems concerning the level of physical training of future officers of the Border Guard Service of Ukraine. The purpose of the article was to analyze the peculiarities of physical training of border guard cadets before and after the full-scale invasion. Methods: The methods used in the research were both theoretical and practical. The results of the research were processed due to the comparative analysis. Results: The recorded changes were taken into account in the content of the Draft Military Doctrine of Ukraine and in the developed Instructions for the Physical Training of Border Guard Officers; in the work educational programs devoted to physical training, personnel policy of the law enforcement agency and the quality of the relevant training sessions, improving the level of physical training of future border guard officers.
Conclusions: The connection between the quality and the content of physical training of future border guard officers in terms of the effectiveness of defensive as well as dynamics and potential of offensive operations during the war was proved subsequent to the results of the research.
Project (e)mergency Remote Instructional Skills Enhancement (e-RISE): Implications to Nursing Education in Times of a Global Health Crisis
Authors: Padagas Reynold Culimay , Gutierrez Ruth , Da Jose Tessie , Bernarte Lotis Melinda
Published: March 2023

The COVID-19 pandemic provokes higher learning institutions to initiate an agile yet well-planned response in ensuring quality online teaching and learning. In response to the challenges of transitioning from the traditional to online teaching and learning, the researchers explored the lessons uncovered from the (e)mergency Remote Instructional Skills Enhancement (e-RISE), an intervention that comprised series of faculty mentoring sessions towards full adoption of an online platform for professional nursing courses. This action research utilized the Look, Think, Act cycle of Stringer (1996) involving eleven nurse faculty as participants.  Using thematic analysis in data treatment, various themes were revealed ranging from advancing technological knowledge and competence to accepting personal limitations, values acquisition, and teacher empowerment. The technological knowledge and competence of the nurse faculty require further improvement.  The acceptance of personal limitations in terms of putting up online learning modules implies the need to enhance pedagogy and design.  Values acquisition in terms of life skills were dominant in response to the needs to change and adapt. Reverse mentoring is also recommended as this is paramount towards the success of the Project e-RISE. Lastly, teacher empowerment is touted essential to make remote teaching and learning more feasible, effective and efficient. The study findings suggest that Project e-RISE be adopted as a sustainable mechanism to capacitate nurse faculty in their migration from traditional to remote or online teaching.
Tourism Local Lore in the Modern System of Education
Authors: Rozhi Inna , Moskalenko Mykola , Moskvichova Olena , Babenko Liudmyla , Nosachenko Volodymyr , Hariunova Yuliia
Published: March 2023

This article covers the main approaches to implementing tourism local lore in the course of education. The problem is relevant due to the demand for competent specialists who are capable to form contemporary children`s competence in the course of education. Therefore, the theoretical-methodological analysis of the researchers of the problem identified, who became the rationale for the main results of the research to be determined, is provided in this paper. It is also ascertained that it is necessary to form a structured part of the professional competence of a rising geography teacher – local lore competence. There is some dependence of the level of qualification of rising teachers on an accurately planned process of formation of not only knowledge, skills and capabilities, but also personal traits of students formed at a higher educational institution. The concepts of native land, local lore and tourism, their correlation, conduction forms, functions are studied in the researches of scholars and according to the results of this theoretical research. In addition, the local lore principle is set as competency-based learning. The method of analysis, scientific, descriptive and research methods are used for studying tourism local lore in the contemporary system of education. Analysing the role of tourism local lore proves the demand for searching and gaining knowledge about a native land, optimising the study of major subjects, forming an individual cognitive experience of students-geographers. Such conclusions are the results of this research.
Voting Yes for Funding Public Parks: The Effectiveness of Social Media Communication in a Tax Referendum Campaign
Authors: Zou Suiwen (Sharon) , Pitas Nicholas , Kerins Andrew , Wuellner Mary Ellen , Jaquet Izabelle
Published: March 2023

Although local park and recreation agencies rely on various types of funding, tax-based allocations are the most significant funding source. Unfortunately, tax-based allocations have not kept pace with growing user demands and maintenance backlogs. As such, successful tax referenda are becoming critical for sustained operations and capital investment. Among the various activities that may sway public opinion, strategic messaging through a variety of media may be one of the most important. Using a county-level public park agency’s 2020 tax referendum as a case study, the purpose of this study is to identify successful communication strategies of open space referendum campaigns that secure support from voters. Data were collected from the campaign’s official Facebook page to examine what message content and post type were effective in increasing voter awareness and engagement. Results from regressions showed that messages that explained the ballot in plain language (e.g., Simplify Ballot Language), demonstrated support or approval from local organizations or local champions/celebrities (e.g., Endorsement), broke down the total amount of tax being requested (i.e., Temporal Reframing), and empha-sized the direct benefits if the referendum were passed (i.e., Direct Outcome) were most effective in increasing the awareness of the referendum. Among Facebook page followers, posts that demonstrated campaign efforts/activities (i.e., Demon-stration) and acknowledged individuals’ or organizations’ efforts to support the campaign (i.e., Acknowledgement) effectively increased engagement. In terms of Facebook post types, photo posts and video posts generally performed better than link and shared video posts. The study has three main practical implications for open space referenda advocates and managers concerned with campaign commu-nication: (1) solicit and demonstrate support from highly regarded local organiza-tions or individuals; (2) emphasize the direct benefits and break down the total amount of tax being asked; and (3) use short videos and scenic images.
A Need for Increased Awareness of Disability Models in Recreational Therapy Practice
Authors: Beaver Shelly
Published: March 2023

Inherently embedded within the descriptions, models, and practices of the field of therapeutic recreation, dis-ability models conceptualize disability, guide therapeu-tic processes and service delivery, and influence client experiences and outcomes. Despite their significance, disability models have received limited attention by the field resulting in inconsistencies within the philosophi-cal underpinnings of the recreational therapy profession. The purpose of this article is to increase awareness of disability models and their implications for recreational therapy practice. A brief conceptual overview of three disability models, including the medical, social, and In-ternational Classification of Functioning, Disability, and Health (ICF) models, is provided, and the models’ cur-rent applications in the field of therapeutic recreation are explored. Utilizing a disability studies lens, a critique of the relationships between each disability model and the core concepts of recreational therapy practice is pre-sented. Commentary is provided to stimulate discussion regarding the need for philosophical consistency of dis-ability values across the profession.
Productivity of intercropping maize with ginger at varying row spacing
Authors:   Bangladesh Agricultural Research Institute, Islam M. K. , Nayeem I. K. A. , Uddin M. Z. , Rahman M. M. , Hossain M. M.
Published: March 2023

The experiment was conducted at Spices Research Sub-Center, BARI, Lalmonirhat in two consecutive years, 2015-16 and 2016-17, to observe the productivity and profitability of intercropping maize with ginger. The experiment was laid out in randomized complete block design with three replications. The treatments were T1: 2 row ginger + 1 row maize (66% ginger + 40% maize), T2: 3 row ginger + 1 row maize (75% ginger + 30% maize), T3: 4 row ginger + 1 row maize (80% ginger + 24% maize), T4: 1 row maize on 3rd ginger row side (100% ginger + 40% maize), T5: 1 row maize on 4th ginger row side (100% ginger + 30% maize), T6: 1 row maize on 5th ginger row side (100% ginger + 24% maize), T7: Sole maize (60cm × 25cm), T8: Sole ginger (50cm × 25cm). Maize was intercropped twice- after harvesting 1st planted maize again, maize was planted into the ginger field. Significant differences were exposed among the treatments regarding yield and intercrop efficiency. Higher ginger equivalent yield (GEY), gross return, gross margin and benefit cost ratio (BCR) were observed in T4, T5 and T6 where maize was planted on 3rd, 4th and 5th ginger row with 100% ginger and in T3 where maize was planted after 4 row ginger. Among those T6 was the most productive and profitable.
Prevalence of Eimeria oocysts among broiler and layer flocks in selected poultry farms in Makurdi, Benue State, Nigeria
Authors:   Joseph Sarwuan Tarka University, Semaka A. A. , Enenche P. O. , Abah H. O.​
Published: March 2023

Coccidiosis is an important enteric parasitic disease associated with significant economic losses to poultry farmers worldwide. This study evaluated the prevalence of coccidian infection in broilers and layers in Makurdi. A total of 228 fresh feacal samples were randomly collected from some selected farms in Makurdi and examined in the laboratory for the presence of Eimeria oocyst using simple floatation technique. The overall prevalence due to natural infection was 58.3%. Out of the 114 broilers and 114 layers sampled 62/114 (54.4%) broilers and 71/114 (62.3%) layers had Eimeria oocysts infection, respectively with layers having the highest prevalence. The age group prevalence showed that 1-4 weeks old broilers and 25-30 weeks layers had the highest prevalence. Significant differences in infection rate were seen in broilers (p˂0.05) but no statistical difference was observed among ages of layer chickens and coccidiostat administration history. The study concludes that the parasite is endemic in Makurdi town and proper control measures with good biosecurity practices and prophylactic anticoccidial programs must be implemented to reduce economic loss to the poultry farmers.
Diseño de grandes almacenes. Claves para un layout exitoso
Authors: Gallardo Joel
Published: March 2023

¿Cómo lograr una tienda armoniosa y un layout que responda a las exigencias de la marca? Este libro contiene la información necesaria para emprender un diseño, así como los aspectos que se deben tomar en cuenta para su producción y ejecución.
Anti-Inflammatory Activity of Stem Barks Ethanol Extracts of Asam Kandis On Male White Rats
Authors: Winata Hanafis Sastra , Andry Muhammad , Nasution Muhammad Amin , Rezaldi Firman , Sembiring Ade Shindy F Br
Published: March 2023

Inflammation is a complex series of changes in tissue due to tissue injury caused by bacteria, trauma, chemicals, heat, and pain. Gamboge (Garcinia xanthochymus) is a fruit-bearing tree native to Southeast Asia. Plants of the genus garcinia are rich in secondary metabolites such as flavonoids, steroids, and triterpenes which have potential pharmacological activity. This study aimed to determine the anti-inflammatory activity based on the reduction in edema volume of Gamboge (Garcinia xanthochymus) stem bark. The inquiry was experimental. Through maceration, an ethanol extract of the gamboge stem was created. The animals were divided into five groups, each containing five white male rats.  The test given was a suspension of gamboge ethanol extract at doses of 200, 400, and 600 mg/kgBW, a negative control of 1% Na CMC suspension, and a positive control of 2.25 mg/kgBW sodium diclofenac. Observations were made for 6 hours, and then data were analyzed using a two-way analysis of variance (ANOVA) test. The results showed that gamboge ethanolic extract was proven to have an anti-inflammatory effect in white male rats induced by carrageenan starting at doses of 200, 400, and 600 mg/kgBW, marked by a significant difference to the negative control (p<0.05). The 600mg/kgBW dose group showed no significantly different results from the positive control group (p>.05). These results concluded that the dose group 600mg/kgBW was the best dose of gamboge stem ethanol extract had an anti-inflammatory activity based on reducing the volume of leg edema of white male rats.
Keywords: Asam Kandis Stem Bark; Anti-Inflammation; Male White Rats
The Effect Of Concentration And Type Of Soaking Media (Water, Alcohol, Salt, And Vinegar) On Decreasing Oxalate Levels (Washability) In Porang
Authors: Sugiarto Bambang , Setyawan Adhi , Nurmalitasari Octavia , Sulistyowati RR Endang
Published: March 2023

Porang tubers (Amorphophallus oncophyllus) contain very high rates of glucomannan and have many benefits in various fields of health, pharmaceutical, industrial, and food fields. Besides having great benefits, porang tubers contain calcium oxalate which causes itching if consumed directly, irritation and kidney stones. The purpose of this study was to make porang tubers free from oxalate content by using various concentrations and types of immersion media on porang. The types of immersion media are water, salt, alcohol, and vinegar. Oxalate rates were analyzed by permanganometric titration method. The results of the diced porang immersion in this study showed that water with a temperature of 70°C with an immersion time of 120 minutes, salt solution at a concentration of 14% with an immersion time of 90 minutes, alcohol with a concentration of 40% with a soaking time of 30 minutes, and vinegar with a concentration of 30% with an immersion time of 150 minutes is the optimal result. While the porang immersion with long slices obtained optimal results in water with a temperature of 50°C with an immersion time of 150 minutes, salt solution at a concentration of 14% with an immersion time of 150 minutes, alcohol with a concentration of 60% with an immersion time of 150 minutes, and vinegar acid with a concentration of 20% with an immersion time of 150 minutes. In the optimal results of the dice, the oxalate content in water immersion decreased by 2.4 %, salt solution decreased by 68.4%, alcohol decreased by 24.6%, and vinegar decreased by 11.8%. In the optimal results of the spring roll filling, the oxalate content in water immersion decreased by 2.4 %, salt solution decreased by 7.3%, alcohol decreased by 19.7%, and vinegar decreased by 11.8%.

 

Keywords:  Porang, Calcium oxalate,Permanganometric
Pembuatan Video Tutorial Sanggul Pengantin Batak Toba Modifikasi Dengan Teknik Serat Nanas Modern
Authors: Sri Sugiarti Sellies Qori Andiani , Titin Supiani
Published: March 2023

Abstrak

Perkembangan trend kecantikan didunia khususnya tata rias rambut mengalami perubahan dan peningkatan. Melalui perkembangan teknologi,  dunia kecantikan dapat dengan mudah dipahami, salah satunya melalui penggunaan media video tutorial Sanggul Pengantin Batak toba modifikasi dengan teknik serat nanas. Tugas Akhir ini bertujuan untuk menghasilkan Video Tutorial Sanggul Pengantin Batak Toba Modifikasi dengan Teknik Serat Nanas Modern yang layak digunakan refrensi dan sumber belajar. Pembuatan video menggunakan 3 tahap yaitu tahap Pra produksi, Produksi dan pasca produksi. Uji kelayakan dilakukan oleh 2 orang yaitu, ahli materi dan ahli media. Pada hasil penilaian keseluruhan dan kesesuaian Video Tutorial Sanggul Pengantin Batak Toba Modifikasi ini menghasilkan penilaian  95% untuk ahli materi dan  97% untuk ahli media yang masuk dalam kategori sangat baik pada hasil keseluruhannya. Berdasarkan hasil tersebut disimpulkan bahwa video tutorialsangat layak di gunakan sebagai refrensi dan sumber belajar bagi mahasiswa Tata Rias Universitas Negeri Jakarta maupun masyarakat umum.
Pengembangan E-Modul Pembuatan Bedak Dingin Dengan Bahan Dasar Beras Dan Ekstrak Singkong
Authors: Syafira Az Zahra  , Mari Okatini Armandari
Published: March 2023

ABSTRAK

Syafira Az Zahra, 1510519022, Pengembangan E-Modul Pembuatan Bedak Dingin Dengan Bahan Dasar Beras dan Ekstrak Singkong, Tugas Akhir: Program Studi D3 Tata Rias, Fakultas Teknik, Universitas Negeri Jakarta, 2022.

Bedak dingin merupakan kosmetika perawatan kulit dari luar secara tradisional karena terbuat dari bahan alami yang telah digunakan secara turun-temurun oleh nenek moyang sehingga teruji khasiatnya. Dalam proses pembuatan bedak dingin terbagi dari berbagai macam bahan dasar, diantaranya beras dan singkong. Hal ini berkaitan dengan salah satu mata kuliah wajib pada Prodi D3 Tata Rias Universitas Negeri Jakarta.  Dalam pembelajarannya diperlukan media yang dapat menunjang pembelajaran jarak jauh sesuai dengan kemajuan era teknologi dan digital seperti e-modul. Dengan tujuan sebagai sumber belajar mahasiswa pada materi Bedak Dingin Dengan Bahan Dasar Beras dan Ekstrak Singkong. Penilaian e-modul terhadap bedak dingin ini dengan dilakukan uji kelayakan oleh 2 ahli, yaitu ahli materi dan ahli media dengan aspek sebagai berikut: aspek kelayakan isi, aspek kelayakan penyajian, aspek kelayakan kontekstual, aspek kelayakan grafik dan aspek kelayakan bahasa. Berdasarkan hasil yang diperoleh dari hasil penilaian para ahli materi 98% dan ahli media 95% dapat disimpulkan bahwa e-modul pembuatan bedak dingin dengan bahan dasar beras dan ekstrak singkong ini sangat layak untuk dijadikan media pembelajaran.
Evaluación de la distancia de los ápices de segundas premolares y molares al conducto dentario inferior según sexo con tomografía computarizada de haz cónico en una población peruana
Authors:   , Manrique Holguín Paola Cecibel
Published: March 2023

El propósito de este estudio fue comparar las distancias promedio desde los ápices radiculares de primeros molares, segundos molares y segundos premolares al canal mandibular según sexo en la población peruana mediante tomografía computarizada de haz cónico (TCHC). Se examinaron 80 tomografías CBCT de pacientes peruanos con edades comprendidas entre los 15 y 80 años. Luego de ubicar el canal mandibular, se realizaron mediciones de las distancias verticales desde el canal mandibular hasta el ápice de los segundos premolares mandibulares, así como de los primeros molares y segundos molares. Para el análisis estadístico se utilizó la prueba t de Student para muestras pareadas y no pareadas con un nivel de significación de p<0,05. En el lado derecho, el segundo molar presentó una distancia media de 3,99 mm para hombres y 2,87 mm para mujeres; exhibiendo una diferencia significativa (p<0.05). Al comparar bilateralmente, no se encontraron diferencias significativas (p>0.05) entre las distancias desde los ápices de los segundos premolares mandibulares y los primeros y segundos molares al canal mandibular. Sin embargo, para los segundos premolares y segundos molares en el lado izquierdo, los valores fueron más altos con un promedio de 5,52 mm y 3,75 mm, respectivamente. Las raíces mesiales de los segundos molares estaban más cerca del canal mandibular. Además, las mujeres mostraron distancias más cortas que los hombres.
Cyclic Liquid Nitrogen Fracturing Performance on Coal with Various Coal Ranks: Laboratory Investigation and Mechanism Analysis
Authors: Yang Ruiyue , Hong Chunyang , Wen Haitao , Huang Zhongwei , Li Gensheng , Lan Tian , Wang Haizhu , Dubinya Nikita
Published: March 2023

Hydraulic fracturing is one of the important stimulation methods to enhance the productivity of coalbed methane (CBM) wells. However, the commonly used water-based fracturing fluids can bring some bottlenecks such as large amount of water consumption, clay-mineral swelling, and poor fracturing performance on ductile coals. Cyclic liquid nitrogen (LN2) fracturing, as a novel nonaqueous stimulation method, has the potential to solve the above problems. In cyclic LN2 fracturing, supercooling LN2 is injected in a cyclic manner [i.e., alternating high injection rate (or pressure) and low injection rate (or pressure)]. Coals will be subjected to cyclic freeze-thaw, stress oscillation, and fatigue damage, which is expected to improve the stimulated reservoir volume. First, laboratory cyclic LN2 fracturing tests were conducted on coal samples with various coal ranks to investigate the fracture initiation/propagation behavior and fracture network patterns. Cyclic water fracturing tests were also conducted as comparisons. Then, computed tomography (CT) scanning and geomechanical/petrophysical properties tests before and after LN2 fracturing were performed to assist in understanding the cyclic LN2 fracturing mechanisms and implications. Finally, to solve the field application concerns, we investigated the possible fracture geometries at the field scale, temperature distribution of LN2 along the wellbore during injection, and the economic feasibility. The key factors affecting the temperature distribution during LN2 transportation along the wellbore were clarified for the first time. The results indicate that cyclic LN2 fracturing shows the potential to decrease the breakdown pressure and produce complex fracture networks. Different coal ranks have different responses to cyclic LN2 fracturing attributed to the variances in natural fracture development and geomechanical/petrophysical properties. Besides, increasing the cycle number is effective in enhancing the cyclic LN2 fracturing performance on coals with relatively higher geomechanical strengths and tighter rock mass. The suggested cycle numbers from low to high for different coal ranks are listed here: low-rank coal < high-rank coal < middle-rank coal. In field applications, gaseous nitrogen (N2) can be used as the annulus fluid to provide an effective insulation for heat transfer between the low-temperature LN2 and the surrounding environment. In addition, the net present value (NPV) analysis indicates that LN2 fracturing is an economically feasible stimulation method, which can exceed slickwater fracturing in some cases. The key findings are expected to provide preliminary insights into the potential field applications of cyclic LN2 fracturing in CBM or other unconventional oil/gas exploitation.
Psychometric properties of Persian version of Columbia Suicide Severity Rating Scale (C-SSRS) in Iranian soldiers
Authors: Jafari Amirhossein , Rahnejat Amir Mohsen , Hooshyari Zahra , Taghva Arsia , Ghasemzadeh Mohammad Reza , Donyavi vahid
Published: March 2023

Background: Suicide is an important health issue, nearly all over the world. Columbia suicide severity rating scale (C-SSRS) is a well-known instrument for suicide risk assessment. Our purpose in this study is to provide a Persian version of C-SSRS and evaluate its psychometric properties in Iranian military population, particularly suicide risk leveling characteristic of C-SSRS.Material & method: for linguistic adaptation, we gathered opinions of expert panel, including 23 professionals in mental health sciences. Furthermore, this version was employed in two groups of soldiers, one of them a sample of normal population (N=338) and the other a sample of clinical population from a referral psychiatric hospital (N=348). Beside the C-SSRS, Beck Scale for Suicidal Ideation (BSSI), Beck hopelessness scale (BSS) and general health questionnaire 28 (GHQ28) was obtained from the participants. Correlation coefficient, internal consistency and factor analysis was evaluated, using Statistical Package for the Social Sciences (version 23) software.Results: all items of Persian version of C-SSRS had acceptable content validity and face validity. This tool had high correlation coefficient with BSSI (r=0.73, P<0.001)and BHS (r=0.64, p<0.001) but low CC with GHQ28 (r=0.22, p<0.001). especially, suicide risk level based on C-SSRS had high correlation with BSSI and BHS. Also, its internal consistency was satisfactory (Cronbach's alpha=0.89). And finally, factor analysis, two factors were obtained that is consistent with suicidal ideation and suicidal behavior factor.Conclusion: our results indicated acceptable validity and reliability for Persian version of C-SSRS and capability of this instrument in classifying the suicide risk. It could be concluded that ordinal suicide risk level (as red, orange, yellow and green) is a valid index for application of C-SSRS.
Ingestion of polystyrene microparticles impairs survival and defecation in larvae of Polistes satan (Hymenoptera: Vespidae)
Authors: De Souza Andre Rodrigues , Bernardes Rodrigo Cupertino , Barbosa Wagner Faria , Viana Thaís Andrade , Nascimento Fábio Santos do , Lima Maria Augusta P. , Martins Gustavo Ferreira
Published: March 2023

Microplastics (MPs) are widespread pollutants of emerging concern, and the risks associated with their ingestion have been reported in many organisms. Terrestrial environments can be contaminated with MPs, and terrestrial organisms, including arthropods, are predisposed to the risk of ingesting MPs. In the current study, the larvae of the paper wasp Polistes satan were fed two different doses (6 mg or 16 mg at once) of polystyrene MPs (1.43 mm maximum length), and the effects of these treatments on immature development and survival till adult emergence were studied. Ingestion of the two doses resulted in mortality due to impaired defecation prior to pupation. The survival of larvae that ingested 16 mg of MPs was significantly lower than that of the control. The ingestion of 16 mg of MPs also reduced the adult emergence (11.4%) in comparison to the control (44.4%). MPs were not transferred from the larvae to the adults that survived. These findings demonstrate that MP ingestion can be detrimental to P. satan, e.g. larval mortality can decrease colony productivity and thus the worker force, and that MPs can potentially affect natural enemies that occur in crops, such as predatory social wasps.
Tracking and Analysis of Initial Noninvasive Prenatal Testing Failure: A Retrospective Study
Authors: Xie Jieqiong , Jiang Yu , Jin Dandan , lu Xingxiu , Xia Zhongmin , Huang Xiaomei , Wen Guiping , Zhou Yulin , Ge Yunsheng
Published: March 2023

Background: To classify and analyze the risk factors associated with initial noninvasive prenatal testing (NIPT) failure in the context of pregnancy outcomes.
Methods: From May 2019 to April 2022, 30039 singleton pregnant women were enrolled in the NIPT, for whom initial failure samples were analyzed and followed up. The initial and final test failure rates were determined. The risk factors of initial test failure were classified as follows: the repeat test success rate and positive predictive values (PPVs) of “z-score in gray area” samples were analyzed; the difference between cases with initial “low fetal fraction (FF)” result and initial “low risk” result, the key factors for success in the second test, and the effect of experimental conditions on the detection rate of low FF samples were assessed; the final NIPT results and pregnancy outcomes of samples with “quality control (QC) failure” were followed up and evaluated.
Results: The total initial failure rate and final failure rate were 2.7% and 0.12% respectively. 99.04% of “z-score in gray area” samples obtained results after repeated test. Of the 22 cases with abnormal or no results, only 15 cases conducted prenatal diagnosis none of which revealed abnormalities with clear clinical significance. Body mass index (BMI), gestational age, library concentration, and fetal sex were considered risk factors for initial low FF results, but were not critical for the success of the second test, or even for initial fetal concentration. The incidence of low FF samples was more frequent with G-type blood collection tubes than with K-type tubes. A total of 99.22% of the QC failure samples obtained interpretable results after resampling or library reconstruction, and all were at low risk.
Conclusions: NIPT has a low final failure rate, but the initial failure rate warrants attention. The PPV of the initial gray area samples was low. Maternal BMI, gestational age, library concentration, fetal sex, and type of blood collection tube may be risk factors for “low FF”.
Influenza A virus uses actomyosin for genome packaging at the cell membrane
Authors: Wang I-Hsuan , Usukura Jiro , Miyake Yasuyuki , Usukura Eiji , Narita Akihiro , Endou Toshiaki , Yamauchi Yohei , Kawaoka Yoshihiro
Published: March 2023

Influenza A virus encodes its genome in eight segments of viral ribonucleopropteins (vRNPs) replicated in the cell nucleus. Clustering of the eight vRNPs is thought to be a selective process promoted by negative-strand RNA interactions. Here, we visualized vRNP packaging at the plasma membrane using immuno-freeze-etching electron microscopy. After being transported to the inner surface of the plasma membrane, individual progeny vRNPs bound to actin filaments and clustered. Clustered vRNPs intermingled with actin filaments, myosin-II and the viral matrix protein M1, as shown by immunocytochemistry. Inhibition of myosin function by blebbistatin prevented proper vRNP clustering. Live-cell high-speed atomic force microscopy revealed blebbistatin reduced the budding kinetics of virions. Our findings highlight the importance of the actomyosin system in vRNP assembly and virion morphogenesis.
The effect of solution focused brief on overcome vaccine hesitancy: A randomized controlled study
Authors: Kaplan Mehmet , ERCİ Behice
Published: March 2023

This study was carried out to determine the effect of solution-focused approach on the elimination of vaccine hesitancy. The research was applied as a quasi-experimental model with pretest-posttest control group. The population of the study included 148 couples (female-male) who did not want to vaccinate their 0-24-month-old babies in Bingöl city, Turkey. Individuals were randomly selected using a simple random sampling method and probability sampling method. The numbers of people who completed the study in the experimental and control groups were 43 and 23, respectively. Four sessions of psychological counseling with a solution-focused brief were applied to each group. The attitude of the parents in the experimental group towards the vaccine changed positively. The confidence in the vaccination of babies between 0-24 months was determine to increase. Although there was no change in the behavior sub-dimension, official records were examined in 1 month after the completion of the study. Six families in the experimental group were determined to vaccinate their children. Solution-focused brief therapy was found to change positively parents’ attitudes and behaviors towards vaccination and to increase healtly lifestyle behaviors.
PpBZR1, a BES/BZR transcription factor, enhances cold stress tolerance by suppressing sucrose degradation in peach fruit
Authors: Zhang Shiyun , Cao Kefeng , Wei Yingying , Jiang Shu , Ye Jianfen , Xu Feng , Chen Yi , Shao Xingfeng
Published: March 2023

Brassinolides (BRs) are phytohormones that play numerous roles in a plants’ response to environmental stress. While BES/BZR transcription factors are essential components in BR signaling, their role in regulating postharvest fruit responses to cold stress is largely unknown. In this study, the application of 24-epibrassinolide (EBR) to peaches alleviated chilling injury (CI) during postharvest cold storage. We further characterized a key BES/BZR gene,PpBZR1, which regulates peach cold resistance. Transient expression PpBZR1in peaches showed that PpBZR1 inhibits PpVIN2 expression and VIN activity, resulting in an elevated level of sucrose, which protects fruit from CI. Arabidopsis expressing PpBZR1 had a high germination and seedling survival rate at low temperatures, which may be due to higher level of sucrose and lower oxidative damage. Mechanistically, we confirmed that PpBZR1 directly binds to the PpVIN2 promoter and functions as a negative regulator. In addition, PpCBF1/5/6 were induced by EBR treatment and AtCBFswere upregulated in transgenic Arabidopsis. Combined with previous findings, we hypothesize that PpBZR1 regulates PpVIN2 through a CBF-dependent manner. In conclusion, PpBZR1 expression is induced by EBR treatment during cold storage, and PpBZR1 futher inhibite sucrose degradation gene PpVIN2transcription via direct binding its promoter and indirectly regulating PpVIN2. This CBF-dependent and CBF-independent regulation results in slower sucrose degradation and higher chilling tolerance of peach.
Bone Disease Imaging through the Near-Infrared-II Window
Authors: Jin Dayong , Mi Chao , Zhang Xun , Yang Chengyu , Wu Jianqun , Chen Xinxin , Ma Chenguang , Wu Sitong , Yang Zhichao , Qiao Pengzhen , Liu Yang , Wu Weijie , Guo Zhiyong , Liao Jiayan , zhou jiajia , Guan Ming , Liang Chao , Liu Chao
Published: March 2023

Bone health related skeletal disorders are commonly diagnosed by X-ray imaging, but the radiation limits its use. Light excitation and optical imaging through the near-infrared-II window (NIR-II, 1000–1700 nm) can penetrate deep tissues without radiation risk, but the targeting of contrast agent is non-specific. Here, we report that lanthanide-doped nanocrystals can be passively transported by endothelial cells and macrophages from the blood vessels into bone marrow microenvironment. We found that this bone targeting scheme can be effective for longer than two months. We therefore developed an intravital 3D and high-resolution planar imaging instrumentation for bone disease diagnosis. We demonstrated the regular monitoring of 1 mm bone defects for 11 days in NIR-II window, with spatial resolution similar to X-ray imaging result, but more flexible use in prognosis. Moreover, the passive targeting can be used to reveal the early onset inflammation at the joints as the synovitis in the early stage of rheumatoid arthritis. Furthermore, the proposed method is comparable to micro computed tomography (µCT) in recognizing symptoms of osteoarthritis, including the mild hyperostosis in femur which is ~ 100 µm thicker than normal, and the growth of millimeter-scale osteophyte in the knee joint, which further proves the power and universality of our approach.
Seasonal analysis of aquatic water quality in a forestry area, MS, Brazil
Authors: Oliveira Víncler Fernandes Ribeiro de Oliveira , Pinto André Luiz Pinto , Lima Cesar Gustavo da Rocha Lima , Américo-Pinheiro Juliana Heloisa Pinê Américo-Pinheiro , Bacani Vitor Matheus Bacani
Published: March 2023

Water quality is one of the world's greatest concerns as it is a fundamental resource for life, and it must constantly be monitored. This study aimed to carry out seasonal monitoring of water quality in the hydrographic basin of the Urutu-MS stream, which is occupied by more than 50% of eucalyptus silviculture forests. Water samples were collected from five points seasonally between autumn 2019 and summer 2020. In total, twenty-three parameters were included, including temperature (Cº), pH, Dissolved Oxygen (DO, mg/L), Conductivity (EC ), Total Alkalinity (ALK), Organic Matter (OM), Chloride (Cl), Sulfate (SO42), Total Iron (Fe Total), Color, Turbidity (NTU), Total Dissolved Solids (TDS), Total Suspended Solids (TSS), Chemical Oxygen Demand (COD), Total Phosphorus (PT), Hardness, Aluminum (Al), Manganese (Mn), Magnesium (Mg), Sodium (Na), Calcium (Ca), Copper (Cu) and Dissolved Iron (Fe). The results showed that there is a difference in water quality between the seasons of the year depending on the different concentrations of physical-chemical parameters. Among the seasons, spring was classified in class II, while the others were classified in class I, showing the influence of the onset of rains after the dry winter. Interactions between parameters also followed conventional associations such as ALK and EC, TDS and EC, Color and Turbidity, TSS and Turbidity, EC and Ca, EC and Mg. The ALK and TDS were the main parameters that showed an influence of seasonality.
PRMT4 interacts with NCOA4 to inhibit ferritinophagy in cisplatin-induced acute kidney injury
Authors: Zhou Lizhi , Wang Yilong , Zhang Hao , Deng Zebin , Yan Shu , Kanwar Yashpal , Wang Yinhuai , Dai Yingbo , Deng Fei
Published: March 2023

Cisplatin-induced acute kidney injury (AKI) is commonly seen in clinical practice. Ferroptosis, an iron-catalyzed non-apoptotic cell death, is operative in the occurrence of cisplatin-induced AKI. Protein arginine methyltransferase (PRMT4), a member of type I PRMT family, was incorporated in various bioprocesses, but its role in renal injuries has not been investigated. In the present study, we aimed to explore the role of PRMT4 in cisplatin-induced AKI and its mechanism involved. Our data showed that PRMT4 was highly expressed in renal proximal tubular cells, and it was downregulated in cisplatin-induced AKI. Besides, genetic disruption of PRMT4 exacerbated, while its overexpression attenuated, cisplatin-induced redox injuries in renal proximal epithelia. Mechanistically, our work showed that PRMT4 interacted with NCOA4 to inhibit ferritinophagy, a process favoring lipid peroxidation to accelerate ferroptosis. Taken together, our study demonstrated that PRMT4 was bound to NCOA4 to attenuate ferroptosis in cisplatin-induced AKI, suggesting that PRMT4 might present as a new therapeutic target for cisplatin-related nephropathy.
Evaluation of expression of UHRF1 and its prognostic and clinicopathological value in human malignancies: A Systematic Review and Meta-analysis
Authors: Shu Qiang , Liu Xiaoling , Mou Tinggang , Xie Fei
Published: March 2023

Background:The mortality and recurrence of patients with cancer is of high prevalence. Ubiquitin-like with plant homeodomain (PHD) and RING finger domains 1 (UHRF1) is a promising nuclear protein gene. There is increasing evidence that UHRF1 is abnormally expressed in various tumors and is associated with cancer prognosis. This study was designed to identify the prognostic value of UHRF1 in human malignancies.Methods:we searched electronic databases up to February 18, 2023, including PubMed, Cochrane Library, Embase, MEDLINE, and Web of Science. Eligible studies that evaluated the clinicopathological and prognostic role of UHRF1 in patients with malignant tumors were included. The pooled odds ratios (ORs) and the hazard ratios (HRs) were calculated to assess the role of UHRF1 using Stata/SE 14.1 software.Results:10 studies included a total of 1461 patients on cancer patients were incorporated into the present meta-analysis. The combined results revealed that high expression of UHRF1 was significantly associated with unfavorable overall survival (OS) (HR=2.01, 95% CI: 1.23-3.29,P=0.005), worse disease free survival(DFS) (HR=1.86, 95% CI: 1.34-2.60,P=0.0002) in a variety of cancers. In additional, the increase in UHRF1 expression was also closely correlated with poor clinical stage (OR = 2.37, 95% CI: 1.59-3.51), lymph node metastasis (OR = 2.86, 95% CI: 1.97-4.16), and tumor recurrence (OR = 3.62, 95% CI: 2.30-5.28) in patients with cancer. Furthermore, the expression datasets of UHRF1 in human malignancies and paired normal tissues were downloaded from Gene Expression Profiling Interactive Analysis(GEPIA) showed that the expression level of UHRF1 was higher in most tumor tissues than in the corresponding normal tissues, which predicted a worse prognosis.The data analysis in Kaplan-meier Plotter also confirmed that patients with UHRF1 upregulation had a worse prognosis.Conclusions:UHRF1 may actas a valuable prognostic biomarker and a potential therapeutic target for patients with cancers.
Removal of thiophene compounds from model fuel with supported copper on activated carbon, adsorption kinetics, and isotherms
Authors: Golipour Haleh , Kazemi Bahador , Mafi Morteza , mokhtarani babak
Published: March 2023

In this study, the adsorption of thiophene compounds (TCs, comprised of thiophene (T), benzothiophene (BT), and dibenzothiophene (DBT)) from model fuel was performed with modified Active Carbon(AC). The single solute model fuel was prepared at 2000ppm, and the mixture concentration of components was performed for 3000ppm. Furthermore, thiophene adsorption from commercial fuel (kerosene) was studied. Based on the experimental results, the correlated parameters of adsorption isotherms, kinetic models, and Fisher factor were calculated. The pseudo-second-order model has the best fitting to experimental data, and the 10% Cu+supported on acid-washed Activated Carbon (A1CN10) has the best-adsorbed amount of TCs (T, BT, and DBT), being 78, 96, 100%, respectively. The physicochemical characterizations for adsorbents were verified by the N2adsorption-desorption surface area analyzer (BET), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Energy Dispersive Spectroscopy (EDS). In addition, the adsorbent-regeneration process was performed by two agitating methods and ultrasound impact was studied.
Biotin-Thiamine Responsive Basal Ganglia Disease: A Retrospective Review of the Clinical, Radiological and Molecular Findings of Cases in Kuwait with Novel Variants
Authors: Aburezq Maryam , Alahmad Ahmad , Alsafi Rasha , Al-Tawari Asma , Ramadan Dina , Shafik Magdy , Abdelaty Omar , Makhseed Nawal , Elshafie Reem , Ayed Mariam , Hayat Abrar , Dashti Fatima , Marafi Dana , Albash Buthaina , Bastaki Laila , Alsharhan Hind
Published: March 2023

Background:Biotin-thiamine-responsive basal ganglia disease (BTRBGD) is a rare autosomal recessive neurometabolic disorder that is caused by biallelic pathogenicSLC19A3variants and characterized by subacute encephalopathy associated with confusion, convulsions, dysphagia, dysarthria, or other neurological manifestations.Methods:A retrospective analysis on the data registry in Kuwait Medical Genetics Center for cases diagnosed clinically, radiographically and confirmed genetically with BTRBGD.Results:Twenty cases from 12 different families were diagnosed with BTRBGD in Kuwait. Age of diagnosis ranges from newborn to 32 years, with a median age of 2 to 3 years. Most cases (83%) presented with confusion, dystonia, convulsions, or dysarthria, while three individuals were diagnosed pre-symptomatically during familial genetic screening. Symptoms resolved completely within 2-week of treatment in two-thirds of the symptomatic cases but progressed in five of them to severe cogwheel rigidity, dystonia and quadriparesis due to delayed presentation and management. Neuroradiological findings of the symptomatic cases revealed bilateral central necrosis of basal ganglia. Two novel homozygous missenseSLC19A3variants were detected in a Kuwaiti and a Jordanian individuals, in addition to the previously reported Saudi founder homozygous variant, c.1264A>G; p.(Thr422Ala) in the remaining cases. All cases are still alive receiving high doses of biotin and thiamine.Conclusion:This is the first study reporting the phenotype and genotype of 20 individuals with BTRBGD in Kuwait and describing two novelSLC19A3variants. It is a treatable neurometabolic disease that requires early recognition and treatment initiation; in addition to the importance of obtaining targeted molecular testing of the founder variant in patients with acute encephalopathy.
Comparison of the AmpFire® Multiplex HPV Assay to the Xpert® HPV Assay for detection of human papillomavirus and cervical disease in women with human immunodeficiency virus: A pragmatic performance evaluation
Authors: Moyo Sikhulile , Ramogola-Masire Doreen , Moraka Natasha , Tawe Leabaneng , Noubary Farzad , Motsumi Kesego , Manowe Godiraone , Zuze Boitumelo , Radibe Botshelo , Hungwe Faith TT , Mohammed Terence , Maphorisa Comfort , Shapiro Roger , Gaseitsiwe Simani , Luckett Rebecca
Published: March 2023

Background: Low- and middle-income countries (LMICs) account for nearly 85% of the global cervical cancer burden, yet have the least access to high-performance screening. International guidelines recommend human papillomavirus testing (HPV) as primary screening, yet implementation is inhibited by the cost of HPV testing.Atila AmpFire® HPV Assay (AmpFire) is both affordable and easy to use, and offers individual genotyping. The objective of this study was to compare the performance of the AmpFire HPV assay to the Xpert® HPV assay in detection of both HPV and clinically significant cervical disease.
Methods: We utilized stored cervical specimens from a prospective cohortstudy of women living with human immunodeficiency virus (HIV) in Botswana conducted from May to July 2018. Positive and negative percent agreement was calculated for the AmpFire and Xpert assays, as was detection of high-grade cervical dysplasia.
Results: 63 stored cervical specimens haddetectable DNA after thawing and were included in the analysis. The positive percent agreement was 91.2% (95%CI: 76.3-98.1) and negative percent agreement was 79.3% (95% CI: 60.3-92.0). Six cases positive by AmpFire but negative by Xpert were HPV genotypes 35, 52 (n=2), 58, 68, and co-infection with HPV 45 and 68. Both Xpert and AmpFire assays detected HPV in all 10 samples of women who had high-grade cervical dysplasia.
Conclusions: The AmpFire HPV assay demonstrated excellent analytic performance in both detection of HPV and clinically significant cervical disease. AmpFire HPV is a promising option to increase access to affordable, type-specific HPV screening for cervical cancer in LMICs.
Plant Disease Classification using Alex Net
Authors: Kewat Krishna
Published: March 2023

—In modern agriculture field, pest and disease identification is a major role of crop cultivation. Image classification by the use of deep convolutional neural networks of training and methodology used the facilitate a quick and easy system implementation. Pests and diseases are a threat to plant production, especially in India, but identification remains to be a challenge in massive scale and automatically. Collecting images from Image Net dataset. The results show that we can effectively detect and recognize the rice diseases and pests including healthy plant class using a deep convolutional neural network, with the best accuracy of 96.50%. The significantly high success rate makes the model a really useful advisory or early warning tool, and an approach that would be further expanded to support an integrated plant disease identification system to work in real cultivation conditions.