bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction Method of the Moisture Content of Black Tea during Processing Based on the Miniaturized Near-Infrared Spectrometer
Authors: Zou Hanting , Shen Shuai , Lan Tianmeng , Sheng Xufeng , Zan Jiezhong , Jiang Yongwen , Du Qizhen , Yuan Haibo
Published: December 2022

The moisture content of black tea is an important factor affecting its suitability for processing and forming the unique flavor. At present, the research on the moisture content of black tea mainly focuses on a single withering step, but the research on the rapid detection method of moisture content of black tea applicable to the entire processing stage is ignored. This study is based on a miniaturized near-infrared spectrometer(micro−NIRS) and establishes the prediction models for black tea moisture content through machine learning algorithms. We use micro−NIRS for spectroscopic data acquisition of samples. Linear partial least squares (PLS) and nonlinear support vector regression (SVR) were combined with four spectral pre−processing methods, and principal component analysis (PCA) was applied to establish the predictive models. In addition, we combine the gray wolf optimization algorithm (GWO) with SVR for the prediction of moisture content, aiming to establish the best prediction model of black tea moisture content by optimizing the selection of key parameters (c and g) of the kernel function in SVR. The results show that SNV, as a method to correct the error of the spectrum due to scattering, can effectively extract spectral features after combining with PCA and is better than other pre−processing methods. In contrast, the nonlinear SVR model outperforms the PLS model, and the established mixed model SNV−PCA−GWO−SVR achieves the best prediction effect. The correlation coefficient of the prediction set and the root mean square error of the prediction set are 0.9892 and 0.0362, respectively, and the relative deviation is 6.5001. Experimental data show that the moisture content of black tea can be accurately and effectively determined by micro-near-infrared spectroscopy.
Further Characterization of Host Preference of Acidovorax citrulli Based on Growth Competition between Group I and Group II Strains
Authors: Yang Yuwen , Qiao Pei , Wang Tielin , Ji Weiqin , Fei Nuoya , Zhang Liqun , Guan Wei , Zhao Tingchang
Published: December 2022

Acidovorax citrulli, the causal agent of bacterial fruit blotch, can be divided into two groups. Group I is mainly isolated from melon, and group II is mainly isolated from watermelon. In this study, group I and II strains were used to assess competition in vivo and in vitro by evaluating inhibition activity assays and by measuring population growth dynamics. Our results indicated that there is no mutual inhibition of growth between the two groups of strains in King’s B medium. The bacterial populations of A. citrulli strains were counted within 7 days after inoculation in melon and watermelon cotyledons and plotted against time to generate population growth curves. Area under the population growth curves was calculated. When the two groups of strains were inoculated separately into melon and watermelon cotyledons, the results of analysis of variance show that there was no significant difference. In this study, for the first time in an experimental setting, we inoculated two A. citrulli groups into melon and watermelon cotyledons at the same time and determined the population dynamics of each strain. The results showed that there was no significant difference between two group strains in melon cotyledons. However, in watermelon cotyledons, the area under population growth curves of group II strain were significantly higher than those of group I strain. Three-way analysis of variance results showed that there was interaction between host and grouping on the influence of strain population level (p < 0.0001). These data provide additional information on the host preference of different groups of A. citrulli.
The Need for a Preparedness Training Model on Disaster Risk Reduction Based on Culturally Sensitive Public Health Nursing (PHN)
Authors: Sofyana Haris , Ibrahim Kusman , Afriandi Irvan , Herawati Erna , Wahito Nugroho Heru Santoso
Published: December 2022

The Indonesian Disaster Risk Index (IRBI) in 2018 found that 52.33% of districts or cities in Indonesia were at high risk of natural disasters and the others were at moderate risk. The World Risk Index places Indonesia at number 33 in the very high-risk category. The policy direction of the Implementation of Disaster Management in Indonesia in 2020–2024 is to increase disaster resilience toward sustainable prosperity for sustainable development. Purpose: This study aims to identify the various needs for a culturally sensitive PHN-based disaster risk-reduction preparedness training model. Methods: This study used a descriptive qualitative research design. Data collection was done through in-depth interviews, Focus Group Discussions (FGDs), and expert panel stages in the Indonesian language. Samples involved in the research included 4 experts and 11 informants. Results: There were 10 themes generated from the results. The analysis results revealed that the level of knowledge, attitudes, and skills of the community is still low. Almost all of the people of Mekar Mukti Village stated that they had never received community-based disaster management training. Conclusions: The study findings highlighted the importance of the Disaster Risk-Reduction Preparedness Model Based on Culturally Sensitive Public Health Nursing for the community.
The Big Five Personality Traits as Predictors of Negative Emotional States in University Students in Taiwan
Authors: Yang Shih-Wei , Koo Malcolm
Published: December 2022

Negative emotional states, such as stress, anxiety, and depression, are prevalent in university students. Personality traits have been shown to be associated with a wide range of behaviors in students, such as academic motivation, achievement, and social well-being. The aim of this study was to investigate the association between the Big Five personality traits and negative emotion states in university students in Taiwan. A cross-sectional study was conducted on 580 university students in Taiwan. Negative emotional states were evaluated using the Depression Anxiety Stress Scale-21 (DASS-21) and the Big Five personality traits were measured using the 48-item Big Five Inventory. A hierarchical linear regression analysis was used to assess the factors associated with DASS-21 scores. Neuroticism (standardized beta [std. β] = 0.45, p < 0.001) and openness (std. β = 0.12, p = 0.003) were significantly associated with DASS-21 scores, while agreeableness (std. β = −0.10, p = 0.007) was significantly and inversely associated with DASS-21 scores. Personality traits could be used to identify students at risk of negative emotional states and to undertake appropriate preventive strategies.
The Impact of SO2 Emissions Trading Scheme on Firm’s Environmental Performance: A Channel from Robot Application
Authors: Song Jian , Wang Yijing , Wang Jing
Published: December 2022

Improving the environmental performance of enterprises is the key to achieving the goal of energy conservation, emission reduction and green development. This paper investigates the causal impact on the firm’s environmental performance of China’s SO2 emissions trading scheme (SO2 ETS), a market-based environmental regulation. Different from the verification mechanism of the Porter hypothesis in the existing literature, we examine the micro mechanism of both emission reduction and efficiency gains of enterprises from the perspective of robot application based on Chinese firm-level data from 2000 to 2013. The paper found that SO2 ETS significantly reduces the emission intensity of Chinese enterprises, and the results are still significant after a series of robustness tests and using instrumental variables to overcome the endogeneity problem. Mechanism analysis shows that the reduction of pollutant emissions and the productivity effect of robot application are two significant channels for SO2 ETS to improve the firm’s environmental performance. In addition, in resource-based and recession-oriented cities, the SO2 ETS has a more significant effect on enterprise emission reduction. These findings provide empirical evidence and policy enlightenment for enterprises to promote market-oriented environmental regulation and release institutional dividends in the process of industrial automation transformation, green and sustainable development.
Influence Mechanism of Polycentric Spatial Structure on Urban Land Use Efficiency: A Moderated Mediation Model
Authors: Zhu Di , Wang Yinghong , Peng Shangui , Zhang Fenglin
Published: December 2022

Under the background of green development, the spatial structure of urban agglomerations (UA) has an important impact on urban land use efficiency (ULUE), but few studies have explored the impact mechanism between the two. This research explores the impacts of polycentric development on ULUE of UA, using data for 140 cities in China’s top ten key UA covering the period from 2004–2019. The linkage between polycentric development and ULUE is explored by estimating models of determinants of ULUE. This research also examines the mechanism of the polycentric spatial structure of UA on ULUE by using a moderated mediation model. The main findings of the research can be concluded as below. The eastern UAs show a mostly polycentric spatial structure, whereas the central and western UAs show a weak polycentric spatial structure. The polycentric spatial structure of UA has a positive impact on ULUE. An inverted U-shape curve depicts the relationship between the polycentric spatial structure of UA and ULUE. However, the mediating variables, integration of industrial structure and factor mobility have a positive and partially mediating effect between the polycentric spatial structure of UA and ULUE. The infrastructure level has a positive U-shaped regulation effect, in which the impact coefficient of transport infrastructure is more significant. These findings provide empirical evidence for the coordinated development of China’s regional space planning and ULUE.
Partly and Fully Supervised Physical Exercise Effects on Cognitive Functions and Movement Proficiency of Adolescents
Authors: Klavina Aija , Veliks Viktors , Gulevska Inta , Aniscenko Aleksandrs , Porozovs Juris , Zusa Anna
Published: December 2022

This study explored the effects of partly supervised physical exercise program (PSPEP) intervention compared to fully supervised physical exercise program (FSPEP) on cognitive functions, movement proficiency and problematic internet use (PIU) in adolescents presenting combined unhealthy lifestyle behaviors. Method: Over a period of 16 weeks the PSPEP group (n = 14) engaged in strength, balance and flexibility exercises three times per week with one supervised session and two unsupervised. The FSPEP group (n = 13) practiced dance activities for 2 to 4 days per week including in training sessions the same exercises as for the PSPEP group. Prior and after the study adolescents completed the PIU scale, performed movement proficiency and cognitive function tests. Results: The PSPEP had significant effect on improvement of stress tolerance (p < 0.01, g = 1.08), while the FSPEP had significant effect on contributing general intelligence (p < 0.05, g = 0.90), color-word interference of the reading speed or color recognition (p < 0.01, g = 1.33), short- and long-term memory (p < 0.01, g = 1.72) and stress tolerance (p < 0.05, g = 1.06). The PSPEP had significant effect on improvement of the bilateral coordination (p < 0.01, g = 1.08). None of the two programs had effect on PIU. Conclusions: Engagement in PSPEP selectively contributed bilateral coordination and cognitive skills related to reaction to multiple stimuli. The FSPEP had multiple significant effects in improvement of cognitive outcomes.
The Impact of Physical Activity at School on Eating Behaviour and Leisure Time of Early Adolescents
Authors: Kolanowski Wojciech , Ługowska Katarzyna , Trafialek Joanna
Published: December 2022

(1) Background: Diet and physical activity (PA) significantly impact health. The aim of this study was to evaluate the impact of long-term increase in organized PA level at school on the eating behaviour and leisure time of early adolescences in the period from the age of 10 to 12. (2) Methods: Children born in 2007 (n = 278) in groups with a standard (control group) and increased level of organized PA at school (4 and 10 h a week) were subjected to an anonymous follow-up survey. The questionnaire included 22 questions related to the eating behaviour and ways and frequency of leisure time PA. The study was conducted in the same groups in three assessment sessions in September 2017, 2018 and 2019. (3) Results: During the course of the study, it was shown improvement of eating behaviour in the increased PA group whereas decline in the standard PA one. The share of children with good and very good scores of eating behaviour decreased in the standard PA group from 56.89% to 54.54% and increased in the increased PA from 58.06% to 60.29%. In the increased PA group children more frequently than standard PA ate vegetables, fruits, fish, poultry meat, wholemeal bakery products, milk, dairy products and drinking tea without sugar, as well as ate breakfast. The standard PA children more frequently than increased PA ate high sugar and/or fat content food as sweets, savoury snacks and drank fizzy drinks as well as fast foods. The frequency of often undertaken leisure time PA increased in the increased PA group from 67% to 74%, while decreased in the standard PA from 58% to 52%. (4) Conclusions: Increase in organized PA at school beneficially influenced early adolescents’ eating behaviour and frequency of leisure time PA during 2 years observation. It also increased early adolescents’ awareness of healthy diet. Increased PA at school helps shaping healthy lifestyle among early adolescents.
Drivers of Stunting Reduction in Yogyakarta, Indonesia: A Case Study
Authors: Siswati Tri , Iskandar Slamet , Pramestuti Nova , Raharjo Jarohman , Rubaya Agus Kharmayana , Wiratama Bayu Satria
Published: December 2022

Background: Chronic malnutrition in children is a severe global health concern. In Yogyakarta, the number of children who are too short for their age has dropped dramatically over the past few decades. Objective: To perform an analysis of trends, policies, and programs; and an assessment of government, community, household, and individual drivers of the stunting reduction in Yogyakarta, Indonesia. Method: Using a mixed-methods approach, there were three types of research: (1) analysis of quantitative data, (2) evaluation of stunting policy, and (3) focus group discussions and in-depth interviews to collect qualitative data. Results: The prevalence of stunting has decreased from year to year. Mean height-for-age z-scores (HAZ) improved by 0.22 SDs from 2013 to 2021. Male and female toddlers aged <20 months have relatively the same body length as the WHO median, but it is lower for children >20 months old. The COVID-19 pandemic has contributed to an increase in stunting-concurrent wasting. Nutrition-specific and -sensitive interventions have been carried out with coverage that continues to increase from year to year, although in 2020, or at the beginning of the COVID-19 pandemic, the coverage of specific interventions decreased. The government has committed to tackling stunting by implementing the five pillars of stunting prevention and the eight convergent stunting actions. As the drivers of stunting reduction, national and community stakeholders and mothers, at the village level, cited a combination of poverty reduction, years of formal education, prevention of early marriage, access to food, enhanced knowledge and perception, and increased access to sanitation and hygiene. Conclusions: Nutrition-specific and -sensitive sector improvements have been crucial for decreasing stunting in Yogyakarta, particularly in the areas of poverty reduction, food access, preventing child marriage, sanitation, education, and increasing knowledge and perception.
Effects of Environmental and Socioeconomic Inequalities on Health Outcomes: A Multi-Region Time-Series Study
Authors: Silva Iara da , Wikuats Caroline Fernanda Hei , Hashimoto Elizabeth Mie , Martins Leila Droprinchinski
Published: December 2022

The gradual increase in temperatures and changes in relative humidity, added to the aging and socioeconomic conditions of the population, may represent problems for public health, given that future projections predict even more noticeable changes in the climate and the age pyramid, which require analyses at an appropriate spatial scale. To our knowledge, an analysis of the synergic effects of several climatic and socioeconomic conditions on hospital admissions and deaths by cardiorespiratory and mental disorders has not yet been performed in Brazil. Statistical analyses were performed using public time series (1996–2015) of daily health and meteorological data from 16 metropolitan regions (in a subtropical climate zone in South America). Health data were stratified into six groups according to gender and age ranges (40–59; 60–79; and ≥80 years old) for each region. For the regression analysis, two distributions (Poisson and binomial negative) were tested with and without zero adjustments for the complete series and percentiles. Finally, the relative risks were calculated, and the effects based on exposure–response curves were evaluated and compared among regions. The negative binomial distribution fit the data best. High temperatures and low relative humidity were the most relevant risk factors for hospitalizations for cardiovascular diseases (lag = 0), while minimum temperatures were important for respiratory diseases (lag = 2 or 3 days). Temperature extremes, both high and low, were the most important risk factors for mental illnesses at lag 0. Groups with people over 60 years old presented higher risks for cardiovascular and respiratory diseases, while this was observed for the adult group (40-59 years old) in relation to mental disorders. In general, no major differences were found in the results between men and women. However, regions with higher urbanization levels presented risks, mainly for respiratory diseases, while the same was observed for cardiovascular diseases for regions with lower levels of urbanization. The Municipal Human Development Index is an important factor for the occurrence of diseases and deaths for all regions, depending on the evaluated group, representing high risks for health outcomes (the value for hospitalization for cardiovascular diseases was 1.6713 for the female adult group in the metropolitan region Palmas, and the value for hospitalization for respiratory diseases was 1.7274 for the female adult group in the metropolitan region Campo Mourão). In general, less developed regions have less access to adequate health care and better living conditions.
Acculturative Stress, Perceived Social Support, and Mental Health: The Mediating Effect of Negative Emotions Associated with Discrimination
Authors: Baeza-Rivera María José , Salazar-Fernández Camila , Manríquez-Robles Diego , Salinas-Oñate Natalia , Smith-Castro Vanessa
Published: December 2022

The role of perceived social support in the acculturation process of immigrants remains unclear. In this study, we jointly evaluated the associations between acculturative stress and negative emotions associated with discrimination as antecedents of anxiety, depression, and stress symptoms in 283 immigrants living in Chile. Three competing models were tested via structural equation modelling to assess (1) the association among these variables and mental health symptoms and (2) to clarify the role of perceived social support. The third model was theoretically more adequate, showed a better fit, and explained 42.7% of the variance of mental health symptoms. In this model, perceived social support was associated with acculturative stress by reducing mental health symptomatology. Moreover, a direct relationship and an indirect relationship were found between acculturative stress (through negative emotions associated with discrimination) and mental health symptomatology. These results contribute to the understanding of the acculturation process experienced by immigrants in Chile and provide empirical evidence to be used to improve migration policies.
Adapting a Physical Earthquake-Aftershock Model to Simulate the Spread of COVID-19
Authors: Gunatilake Thanushika , Miller Stephen A.
Published: December 2022

There exists a need for a simple, deterministic, scalable, and accurate model that captures the dominant physics of pandemic propagation. We propose such a model by adapting a physical earthquake/aftershock model to COVID-19. The aftershock model revealed the physical basis for the statistical Epidemic Type Aftershock Sequence (ETAS) model as a highly non-linear diffusion process, thus permitting a grafting of the underlying physical equations into a formulation for calculating infection pressure propagation in a pandemic-type model. Our model shows that the COVID-19 pandemic propagates through an analogous porous media with hydraulic properties approximating beach sand and water. Model results show good correlations with reported cumulative infections for all cases studied. In alphabetical order, these include Austria, Belgium, Brazil, France, Germany, Italy, New Zealand, Melbourne (AU), Spain, Sweden, Switzerland, the UK, and the USA. Importantly, the model is predominantly controlled by one parameter (α), which modulates the societal recovery from the spread of the virus. The obtained recovery times for the different pandemic waves vary considerably from country to country and are reflected in the temporal evolution of registered infections. These results provide an intuition-based approach to designing and implementing mitigation measures, with predictive capabilities for various mitigation scenarios.
Endotoxin as a Marker for Water Quality
Authors: Sattar Anas A. , Good Christian R. , Saletes Margaux , Brandão João , Jackson Simon K.
Published: December 2022

Background: Water quality testing is vital to protect human health. Current testing relies mainly on culture-based detection of faecal indicator organisms such as Escherichia coli (E.coli). However, bacterial cultures are a slow process, taking 24–48 h and requiring specialised laboratories and trained personnel. Access to such laboratories is often sparse in developing countries and there are many fatalities deriving from poor water quality. Endotoxin is a molecular component of Gram-negative bacterial cell walls and can be used to detect their presence in drinking water. Method: The current study used a novel assay (BacterisK) to rapidly detect endotoxin in various water samples and correlate the results with E. coli content measured by culture methods. The data generated by the BacterisK assay are presented as an ‘endotoxin risk’ (ER). Results: The ER values correlate with E. coli and thus endotoxin can be used as a marker of faecal contamination in water. Moreover, the BacterisK assay provides data in near real-time and can be used in situ allowing water quality testing at different spatial and temporal locations. Conclusion: We suggest that BacterisK can be used as a convenient risk assessment tool to assess water quality where results are required quickly or access to laboratories is lacking.
Comparison of the Application of Three Methods for the Determination of Outdoor PM2.5 Design Concentrations for Fresh Air Filtration Systems in China
Authors: Zhang Xin , Sun Hao , Li Kaipeng , Nie Xingxin , Fan Yuesheng , Wang Huan , Ma Jingyao
Published: December 2022

With the increasing popularity of fresh-air filtration systems, the methods of determining the outdoor PM2.5 design concentration have become more important. However, the monitoring of atmospheric fine particles in China started relatively late, and there are relatively few cities with complete data, with obvious regional differences, which led to many problems in the selection of air filters for fresh-air filtration systems. In this paper, three methods of determining outdoor PM2.5 design concentration were analyzed using the daily average concentration of PM2.5 in 31 provincial capital cities from 2016 to 2020. Six typical cities in different regions were also taken as examples. The advantages and disadvantages of the three existing statistical methods were compared and analyzed, as well as the corresponding differences in the selection of outdoor PM2.5 concentration value on the filter systems. The results showed that the method of mathematical induction was more accurate and reasonable for the calculation of outdoor PM2.5 design concentrations. The local outdoor PM2.5 design concentration could be quickly calculated using the recommended coefficient K and annual average PM2.5 concentration of the region, especially for small and medium-sized cities without monitoring data. However, the recommended coefficient K should be provided based on the specific region, and should be divided into values for strict conditions and normal conditions during use. This would provide a simple and effective way to select the correct air filters for practical engineering.
How to Achieve Carbon Neutrality: From the Perspective of Innovative City Pilot Policy in China
Authors: Liu Lina , Zhang Yunyun , Liu Bei , Xiu Pishi , Sun Lipeng
Published: December 2022

The innovative city pilot policy is a new engine to accelerate the social development of China, which is an important support feature for realizing sustainable economic development. Using the city pilot policy issued by the Chinese government in 2008 as a quasi-natural experiment and the method of multi-period difference-in-differences (DID) model, we explore the effect of the policy on regional carbon emission efficiency. The research shows that the innovative city pilot policy could lead a significant promotion of the carbon emission efficiency of cities, which shows the characteristics of dynamic sustainability, that is, the policy effect continues to increase over time. Mechanism analysis reveals that the innovative city pilot policy mainly drives the improvement of urban carbon emission efficiency through improving the green technology innovation level of pilot cities, promoting the upgrading of regional industrial structure and increasing government investment in science and technology. In addition, the innovative city pilot policy has a spatial spillover effect on urban carbon emission efficiency, that is, the innovative city pilot policy not only promotes the local carbon emission efficiency, but also improves the carbon efficiency of neighboring areas.
Evaluation of Emergency Response Capacity of Urban Pluvial Flooding Public Service Based on Scenario Simulation
Authors: Zhang Yongling , Zhou Miao , Kong Nana , Li Xin , Zhou Xiaobing
Published: December 2022

The evaluation of emergency response capability under different pluvial flooding scenarios is an essential approach to improve the emergency response capability of flood disasters. A new evaluation method of emergency response capacity of urban public services is proposed based on urban pluvial flooding scenario simulation. Firstly, inundation area and depth under different pluvial flooding scenarios are simulated based on the SCS-CN model. Following that, space densities of all indicators include inundation area and depth, road network and the emergency public service institutions. Then, the indicator weight is determined by the combined weighting method of entropy weight and coefficient of variation. Finally, the emergency response capacity index (of each pixel) is calculated based on the graph stacking method. Taking Erqi District, Zhengzhou City as an example, the emergency response capacity of public service under different urban flooding scenarios is evaluated. The results show that the spatial distribution difference of public service emergency response capacity in Erqi District, Zhengzhou City is obvious, and with the increase of the precipitation return period, the high value area of public service emergency response capability decreases gradually and the low value area increases gradually. This method takes into account the specific urban flooding scenario and the layout of public service institutions and road networks that have strong practicability. the results of the evaluation can provide a reference for the construction of urban flood emergency response capacity and provide support for emergency decision-making.
Comparing Children’s Behavior Problems in Biological Married, Biological Cohabitating, and Stepmother Families in the UK
Authors: Hveem M. Rachél , Faulconer Samuel C. M. , Dufur Mikaela J.
Published: December 2022

A large body of research shows that children who live with two married biological parents have lower levels of externalizing and internalizing behavior problems compared to their peers in other family structure, including cohabitating biological families. Such patterns suggest that marriage provides a uniquely protective family environment, though we know less about children in the obvious counterfactual case: married stepfamilies. While research suggests children with stepfathers have more behavior problems than those living with married biological parents, we know little about how children with stepmothers fare, or how children with stepparents fare compared to those living with cohabiting biological parents. We use the Millennium Cohort Study (MCS) sweep 6 to compare children living with married biological parents, married fathers and stepmothers, and cohabiting biological parents. We find that family structure has no significant relationship with children’s internalizing behavior problems, but that children living with a stepmother and biological cohabitating families exhibit more externalizing behavior problems than do those living with married biological parents. Covariates that indicate both physical and social family environments must be considered together to explain differences in married-parent families on externalizing behavior problems.
COVID-19 Progression: A County-Level Analysis of Vaccination and Case Fatality in Mississippi, USA
Authors: Ghosh Shinjita , Ahmad Hafiz A. , Akil Luma , Tchounwou Paul B.
Published: December 2022

The COVID-19 pandemic has created a severe upheaval in the U.S., with a particular burden on the state of Mississippi, which already has an exhausted healthcare burden. The main objectives of this study are: (1) to analyze the county-level COVID-19 cases, deaths, and vaccine distribution and (2) to determine the correlation between various social determinants of health (SDOH) and COVID-19 vaccination coverage. We analyzed COVID-19-associated data and county-level SDOH factors in 82 counties of Mississippi. The cumulative COVID-19 and socio-demographic data variables were grouped into feature and target variables. The statistical and exploratory data analysis (EDA) was conducted using Python 3.8.5. The correlation between the target and feature variables was performed by Pearson Correlation analysis. The heat Map Correlation Matrix was visually presented to illustrate the correlation between each pair of features and each target variable. Results indicated that people of Asian descent had the highest vaccination coverage of 77% fully vaccinated compared to 52%, 46%, 42% and 25% for African Americans, Whites, Hispanics, and American Indians/Alaska Natives, respectively. The county-level vaccination rate was significantly higher among the minority populations than the White population. It was observed that COVID-19 cases and deaths were positively correlated with per capita income and negatively correlated with the percentage of persons without a high school diploma (age 25+). This study strongly demonstrates that different SDOH factors influence the outcome of the COVID-19 vaccination rate, which also affects the total number of COVID-19 cases and deaths. Vaccine promotion should be given to all populations regardless of race and ethnicity to achieve uniform acceptance. Therefore, statewide policy recommendations focusing on specific community needs should help achieve health equity in COVID-19 vaccination management.
The Control of Novel and Traditional Elemental Impurities: Ag, Au, Co, Cs, Li, Mo, Se, Sr, and V in Mint Tea Infusions (Peppermint, Mentha piperita L.) Available in Poland: A Health Risk Assessment
Authors: Milan Justyna , Frydrych Adrian , Noga Maciej , Kondratowicz-Pietruszka Elżbieta , Krośniak Mirosław , Jurowski Kamil
Published: December 2022

The purpose of our studies is the evaluation of the health risks of the novel elemental impurities (Ag, Au, Co, Cs, Li, Mo, Se, Sr, and V) in mint tea infusions (Mentha piperita L.) available in Poland. For this purpose, we applied ICP-MS-based methodology for content analysis (elemental profile and µg/L of the infusion) and also the specific health risk assessment for a comprehensive assessment. Our strategy was based on weekly intake (µg/L of infusion/week) considering weekly tea consumption. Then, based on the weekly consumption of tea per adult, individuals were compared to the Temporary Tolerated Weekly Intake (PTWI) designated by the Joint FAO Expert Committee on Food Additives (JECFA), and the weekly consumption per body weight (µg/L of infusion/week/bw) was estimated. Daily exposure to Co in the tested products is in a range of 0.093–0.238 µg/day. In comparison, the established PDE (oral exposure) for Co by the ICH guideline (the ICH guideline Q3D (R1), 2019) is 50 µg/day. For lithium, PDE is approximately 560 µg/day and, in our study, the estimated daily exposure for Li in investigated products is in a range of 0.226–2.41 µg/day. Our studies found also low levels of Cs (in a range of 0.0598–0.195 µg/L), Mo (in a range of 0.663–3.261 µg/L), and Sr (0.223–65.842 µg/L) in infusions. For Molybdenum, the established PDE is approximately 3400 µg/day. There are no documents about Sr and Co in the Joint FAO/WHO Expert Committee on Food Additives (JECFA) database and the ICH guideline Q3D (R1). In the case of V, the established PDE is 120 µg/day, and the level of V in analyzed infusions is in a range of 0.284–0.702 µg/L. Silver and gold were present only in a few samples, and the estimated daily exposure for Ag is approximately 0.04575 µg/day for product A and approximately 0.1885 µg/day for product O, except for Au, which is in a range of 0.000356–0.114 µg/L. The estimated daily exposure for Ag is 167 µg/day and, for Au, it is in a range of 8.9 × 105–0.0285 µg/day. It can be concluded that levels of all investigated elements (novel and also traditional elemental impurities) in the daily dose should not threaten the consumer’s health after consuming mint tea infusions.
TRIAC Treatment Improves Impaired Brain Network Function and White Matter Loss in Thyroid Hormone Transporter Mct8/Oatp1c1 Deficient Mice
Authors: Reinwald Jonathan Rochus , Weber-Fahr Wolfgang , Cosa-Linan Alejandro , Becker Robert , Sack Markus , Falfan-Melgoza Claudia , Gass Natalia , Braun Urs , Clemm von Hohenberg Christian , Chen Jiesi , Mayerl Steffen , Muente Thomas F. , Heuer Heike , Sartorius Alexander
Published: December 2022

Dysfunctions of the thyroid hormone (TH) transporting monocarboxylate transporter MCT8 lead to a complex X-linked syndrome with abnormal serum TH concentrations and prominent neuropsychiatric symptoms (Allan-Herndon-Dudley syndrome, AHDS). The key features of AHDS are replicated in double knockout mice lacking MCT8 and organic anion transporting protein OATP1C1 (Mct8/Oatp1c1 DKO). In this study, we characterize impairments of brain structure and function in Mct8/Oatp1c1 DKO mice using multimodal magnetic resonance imaging (MRI) and assess the potential of the TH analogue 3,3′,5-triiodothyroacetic acid (TRIAC) to rescue this phenotype. Structural and functional MRI were performed in 11-weeks-old male Mct8/Oatp1c1 DKO mice (N = 10), wild type controls (N = 7) and Mct8/Oatp1c1 DKO mice (N = 13) that were injected with TRIAC (400 ng/g bw s.c.) daily during the first three postnatal weeks. Grey and white matter volume were broadly reduced in Mct8/Oatp1c1 DKO mice. TRIAC treatment could significantly improve white matter thinning but did not affect grey matter loss. Network-based statistic showed a wide-spread increase of functional connectivity, while graph analysis revealed an impairment of small-worldness and whole-brain segregation in Mct8/Oatp1c1 DKO mice. Both functional deficits could be substantially ameliorated by TRIAC treatment. Our study demonstrates prominent structural and functional brain alterations in Mct8/Oatp1c1 DKO mice that may underlie the psychomotor deficiencies in AHDS. Additionally, we provide preclinical evidence that early-life TRIAC treatment improves white matter loss and brain network dysfunctions associated with TH transporter deficiency.
The Cytoskeleton in Plant Immunity: Dynamics, Regulation, and Function
Authors: Wang Jingyi , Lian Na , Zhang Yue , Man Yi , Chen Lulu , Yang Haobo , Lin Jinxing , Jing Yanping
Published: December 2022

The plant cytoskeleton, consisting of actin filaments and microtubules, is a highly dynamic filamentous framework involved in plant growth, development, and stress responses. Recently, research has demonstrated that the plant cytoskeleton undergoes rapid remodeling upon sensing pathogen attacks, coordinating the formation of microdomain immune complexes, the dynamic and turnover of pattern-recognizing receptors (PRRs), the movement and aggregation of organelles, and the transportation of defense compounds, thus serving as an important platform for responding to pathogen infections. Meanwhile, pathogens produce effectors targeting the cytoskeleton to achieve pathogenicity. Recent findings have uncovered several cytoskeleton-associated proteins mediating cytoskeletal remodeling and defense signaling. Furthermore, the reorganization of the actin cytoskeleton is revealed to further feedback-regulate reactive oxygen species (ROS) production and trigger salicylic acid (SA) signaling, suggesting an extremely complex role of the cytoskeleton in plant immunity. Here, we describe recent advances in understanding the host cytoskeleton dynamics upon sensing pathogens and summarize the effectors that target the cytoskeleton. We highlight advances in the regulation of cytoskeletal remodeling associated with the defense response and assess the important function of the rearrangement of the cytoskeleton in the immune response. Finally, we propose suggestions for future research in this area.
A Novel Ag@AgCl Nanoparticle Synthesized by Arctic Marine Bacterium: Characterization, Activity and Mechanism
Authors: Li Shuang , Zhang Hui , Cong Bailin , He Peiqing , Liu Wenqi , Liu Shenghao
Published: December 2022

An additive- and pollution-free method for the preparation of biogenic silver and silver chloride nanoparticles (Ag@AgCl NPs) was developed from the bacteria Shewanella sp. Arc9-LZ, which was isolated from the deep sea of the Arctic Ocean. The optimal synthesizing conditions were explored, including light, pH, Ag+ concentration and time. The nanoparticles were studied by means of ultraviolet-visible (UV-Vis) spectrophotometry, energy dispersive spectrometry (EDS), X-ray diffraction (XRD) and inductively coupled plasma optical emission spectrometers (ICP-OES). The transmission electron microscope (TEM) showed that the nanoparticles were spherical and well dispersed, with particle sizes less than 20.00 nm. With Ag@AgCl nanoparticles, the kinetic rate constants for congo red (CR) and rhodamine B (RhB) dye degradation were 2.74 × 10−1 min−1 and 7.78 × 10−1 min−1, respectively. The maximum decolourization efficiencies of CR and RhB were 93.36% and 99.52%, respectively. Ag@AgCl nanoparticles also showed high antibacterial activities against the Gram-positive and Gram-negative bacteria. The Fourier transform infrared spectroscopy (FTIR) spectrum indicated that the O-H, N-H and -COO- groups in the supernatant of Arc9-LZ might participate in the reduction, stabilization and capping of nanoparticles. We mapped the schematic diagram on possible mechanisms for synthesizing Ag@AgCl NPs.
Identification of JPX-RABEP1 Pair as an Immune-Related Biomarker and Therapeutic Target in Pulmonary Arterial Hypertension by Bioinformatics and Experimental Analyses
Authors: Gong Qian , Hu Zhewei , Jin Qiao , Yan Yan , Liu Yan , He Jin , Zhuang Lenan , Wang Huanan
Published: December 2022

Pulmonary arterial hypertension (PAH) is a pulmonary vascular disease characterized by pulmonary vascular remodeling and right heart enlargement the pathogenesis of PAH is complicated; no biologic-based therapy is available for the treatment of PAH, but recent studies suggest that inflammatory response and abnormal proliferation of pulmonary artery smooth muscle cells are the main pathogenic mechanism, while the role of immune-related long non-coding RNAs (lncRNAs) remains unclear. The aim of this study was to systematically analyze immune-related lncRNAs in PAH. Here, we downloaded a publicly available microarray data from PAH and control patients (GSE113439). A total of 243 up-regulated and 203 down-regulated differentially expressed genes (DEGs) were screened, and immune-related DEGs were further obtained from ImmPort. The immune-related lncRNAs were obtained by co-expression analysis of immune-related mRNAs. Then, immune-related lncRNAs-mRNAs network including 2 lncRNAs and 6 mRNAs was constructed which share regulatory miRNAs and have significant correlation. Among the lncRNA-mRNA pairs, one pair (JPX-RABEP1) was verified in the validating dataset GSE53408 and PAH mouse model. Furthermore, the immune cell infiltration analysis of the GSE113439 dataset revealed that the JPX-RABEP1 pair may participate in the occurrence and development of PAH through immune cell infiltration. Together, our findings reveal that the lncRNA-mRNA pair JPX-RABEP1 may be a novel biomarker and therapeutic target for PAH.
High-Throughput Sequencing Reveals Novel microRNAs Involved in the Continuous Flowering Trait of Longan (Dimocarpus longan Lour.)
Authors: Waheed Saquib , Liang Fan , Zhang Mengyuan , He Dayi , Zeng Lihui
Published: December 2022

A major determinant of fruit production in longan (Dimocarpus longan Lour.) is the difficulty of blossoming. In this study, high-throughput microRNA sequencing (miRNA-Seq) was carried out to compare differentially expressed miRNAs (DEmiRNAs) and their target genes between a continuous flowering cultivar ‘Sijimi’ (SJ), and a unique cultivar ‘Lidongben’ (LD), which blossoms only once in the season. Over the course of our study, 1662 known miRNAs and 235 novel miRNAs were identified and 13,334 genes were predicted to be the target of 1868 miRNAs. One conserved miRNA and 29 new novel miRNAs were identified as differently expressed; among them, 16 were upregulated and 14 were downregulated. Through the KEGG pathway and cluster analysis of DEmiRNA target genes, three critical regulatory pathways, plant–pathogen interaction, plant hormone signal transduction, and photosynthesis-antenna protein, were discovered to be strongly associated with the continuous flowering trait of the SJ. The integrated correlation analysis of DEmiRNAs and their target mRNAs revealed fourteen important flowering-related genes, including COP1-like, Casein kinase II, and TCP20. These fourteen flowering-related genes were targeted by five miRNAs, which were novel-miR137, novel-miR76, novel-miR101, novel-miR37, and csi-miR3954, suggesting these miRNAs might play vital regulatory roles in flower regulation in longan. Furthermore, novel-miR137 was cloned based on small RNA sequencing data analysis. The pSAK277-miR137 transgenic Arabidopsis plants showed delayed flowering phenotypes. This study provides new insight into molecular regulation mechanisms of longan flowering.
Attenuation of Smooth Muscle Cell Phenotypic Switching by Angiotensin 1-7 Protects against Thoracic Aortic Aneurysm
Authors: Jadli Anshul S. , Ballasy Noura N. , Gomes Karina P. , Mackay Cameron D. A. , Meechem Megan , Wijesuriya Tishani Methsala , Belke Darrell , Thompson Jennifer , Fedak Paul W. M. , Patel Vaibhav B.
Published: December 2022

Thoracic aortic aneurysm (TAA) involves extracellular matrix (ECM) remodeling of the aortic wall, leading to reduced biomechanical support with risk of aortic dissection and rupture. Activation of the renin-angiotensin system, and resultant angiotensin (Ang) II synthesis, is critically involved in the onset and progression of TAA. The current study investigated the effects of angiotensin (Ang) 1-7 on a murine model of TAA. Male 8–10-week-old ApoEKO mice were infused with Ang II (1.44 mg/kg/day) and treated with Ang 1-7 (0.576 mg/kg/day). ApoEKO mice developed advanced TAA in response to four weeks of Ang II infusion. Echocardiographic and histological analyses demonstrated increased aortic dilatation, excessive structural remodelling, perivascular fibrosis, and inflammation in the thoracic aorta. Ang 1-7 infusion led to attenuation of pathological phenotypic alterations associated with Ang II-induced TAA. Smooth muscle cells (SMCs) isolated from adult murine thoracic aorta exhibited excessive mitochondrial fission, oxidative stress, and hyperproliferation in response to Ang II. Treatment with Ang 1-7 resulted in inhibition of mitochondrial fragmentation, ROS generation, and hyperproliferation. Gene expression profiling used for characterization of the contractile and synthetic phenotypes of thoracic aortic SMCs revealed preservation of the contractile phenotype with Ang 1-7 treatment. In conclusion, Ang 1-7 prevented Ang II-induced vascular remodeling and the development of TAA. Enhancing Ang 1-7 actions may provide a novel therapeutic strategy to prevent or delay the progression of TAA.
Naïve CD4+ T Cell Activation in the Nasal-Associated Lymphoid Tissue following Intranasal Immunization with a Flagellin-Based Subunit Vaccine
Authors: Bates John T.
Published: December 2022

The nasal-associated lymphoid tissues (NALT) are generally accepted as an immune induction site, but the activation of naïve T-cells in that compartment has not been well-characterized. I wanted to determine if early events in naïve CD4+ T cell activation and the extent of antigen specific cell division are similar in NALT to that observed in other secondary lymphoid compartments. I performed antigen tracking experiments and analyzed the activation of naïve antigen-specific CD4+ T cells in the nasal-associated lymphoid tissues (NALT). I directly observed transepithelial transport of fluorescently labeled antigen from the lumen of the airway to the interior of the NALT two hours following immunization. One day following intranasal (i.n.) immunization with antigen and adjuvant, antigen-specific CD4+ T cells in the NALT associated as clusters, while antigen-specific CD4+ T cells in control mice immunized with adjuvant only remained dispersed. The antigen-specific CD4+ populations in the NALT and cranial deep cervical lymph nodes of immunized mice expanded significantly by day three following immunization. These findings are consistent with initial activation of naïve CD4+ T cells in the NALT and offer insight into adjuvant mechanism of flagellin in the upper respiratory compartment.