bluuebunny/crossref_metadata_2025 huggingface dataset:
The Addition of an Invasive Plant Alters the Home-Field Advantage of Native Leaf Litter Decomposition
Authors: Chen Shaojun , Xie Xiaohua , Wen Jie , Zhai Hao , Wang Huiqi , Jiang Yuhang , Gou Zhanxu
Published: September 2024

Forest litter can decompose faster at home sites than at guest sites (home-field advantage, HFA), yet few studies have focused on the response of the HFA of native plant decomposition to the presence of invasive plants. We loaded the dry leaves of native Neosinocalamus affinis (decomposition resistant) and Ficus virens (more easily decomposable) leaves into litterbags with and without invasive Alternanthera philoxeroides, and incubated these litterbags at N. affinis and F. virens sites at the edge of the forest. The results showed that positive HFA effects with litter mass loss were at least 1.32% faster at home sites than at guest sites. The addition of A. philoxeroides reduced the mean HFA of N. affinis litter and increased that of F. virens litter. The HFA index without A. philoxeroides was significantly higher than that with A. philoxeroides. Soil faunal abundance colonized at home sites was always higher than that colonized at guest sites. Compared with the F. virens site, the abundance of Collembola, Arachnida, Formicidae and Lepismatidae at the N. affinis site was significantly higher compared to the F. virens site, while the abundance of Isopoda, Oligochaeta, Nematoda and Dermaptera was significantly lower. Our results indicate that invasive plants may regulate HFA effects by promoting the decomposition of native plants and increasing fauna abundance. Particularly, soil fauna groups play a very important role in this process. Our findings help us to re-understand the role of invasive plants in material cycling and energy flow in the context of achieving carbon neutrality goals.
Growing Jatropha curcas L. Improves the Chemical Characteristics of Degraded Tropical Soils
Authors: Massoukou Pamba Renaud , Poirier Vincent , Nguema Ndoutoumou Pamphile , Epule Terence Epule
Published: September 2024

Intensive agriculture in tropical regions is the main cause of soil impoverishment, reducing its productivity. Studies based on soil restoration methods are being implemented, including the use of plants such as Jatropha curcas L., which could have the capacity to improve the agronomic properties of degraded soils in the tropics. The aim of this study is, therefore, to demonstrate that J. curcas L. can improve the characteristics of degraded tropical soil. Between October 2019 and November 2022, we evaluated the effect of spacing, planting material type and age, as well as their interactions, on carbon (C) and nitrogen (N) concentrations and pH at two depths (i.e., 0–10 and 10–20 cm) in the soil. The results reveal that after three years of J. curcas L. growth, C concentration and soil pH increased significantly (p < 0.001) at both depths, while N concentration increased significantly between 0 and 10 cm only. Plants grown from cuttings improved soil pH at 10–20 cm depth more (p = 0.012) than those grown from seeds. Three years after planting, soil N concentration under J. curcas reached a value comparable to that of undisturbed adjacent soil. Overall, our results indicate that J. curcas is a plant that can contribute effectively to restoring degraded tropical soils, therefore contributing to limiting the deforestation of natural forests.
Digital Transformation in Maritime Ports: Defining Smart Gates through Process Improvement in a Portuguese Container Terminal
Authors: Basulo-Ribeiro Juliana , Pimentel Carina , Teixeira Leonor
Published: September 2024

As the digital paradigm stimulates changes in various areas, seaports, which are fundamental to logistics and the global supply chain, are also undergoing a digital revolution, evolving into smart ports. Smart gates are essential components in this transformation, playing a vital role in increasing port efficiency. In the context of smart gates, the aim of this study is to understand how process management can serve as a catalyst for digital transformation, promoting efficiency in traffic flow and logistics. To achieve this objective, the design science research (DSR) methodology was followed, which allowed for the integration of information from several sources of requirement, encompassing both theoretical and practical aspects. The practical component took place at one of Portugal’s largest container terminals, which allowed for the integration of information from various sources. As a result, this study presents the conceptual definition of a smart gate in terms of processes, main technologies, and key performance indicators that will support the monitoring and improvement of future operations. The results provide theoretical and practical contributions: on a practical level, they present a real application of the transformation towards a smart gate, serving as a model for other ports in their digitalization; on a theoretical level, they enrich the literature with a methodology for digitalizing maritime road gates, showing how the use of process management approaches, such as the BPMN, can increase operational efficiency in container terminals.
Automated Detection of Misinformation: A Hybrid Approach for Fake News Detection
Authors: Mohsen Fadi , Chaushi Bedir , Abdelhaq Hamed , Karastoyanova Dimka , Wang Kevin
Published: September 2024

The rise of social media has transformed the landscape of news dissemination, presenting new challenges in combating the spread of fake news. This study addresses the automated detection of misinformation within written content, a task that has prompted extensive research efforts across various methodologies. We evaluate existing benchmarks, introduce a novel hybrid word embedding model, and implement a web framework for text classification. Our approach integrates traditional frequency–inverse document frequency (TF–IDF) methods with sophisticated feature extraction techniques, considering linguistic, psychological, morphological, and grammatical aspects of the text. Through a series of experiments on diverse datasets, applying transfer and incremental learning techniques, we demonstrate the effectiveness of our hybrid model in surpassing benchmarks and outperforming alternative experimental setups. Furthermore, our findings emphasize the importance of dataset alignment and balance in transfer learning, as well as the utility of incremental learning in maintaining high detection performance while reducing runtime. This research offers promising avenues for further advancements in fake news detection methodologies, with implications for future research and development in this critical domain.
Generating Process Models by Interacting with Chatbots—A Literature Review
Authors: Hörner Luca Franziska , Reichert Manfred
Published: September 2024

This paper presents a comprehensive literature review, focusing on the emerging intersection of chatbot technology and the generation of process models. As an evolving field of study, the integration of interactive chatbots into process model generation represents a promising approach, blending advancements in artificial intelligence in general, and natural language processing in particular, with process management methods. This review systematically examines the existing literature across multiple disciplines, identifying and analyzing studies that touch upon the individual components of this nascent topic: chatbot technology, process model generation, and their synergistic potential. Despite the scarcity of direct research aimed at using chatbots for process model generation, this review synthesizes relevant findings from related domains, such as natural language processing applications in process modeling, and the broader impact of chatbot interfaces in various domains. Through this analysis, we aim to map the current landscape of research, highlight significant gaps, and suggest potential pathways for future investigations. This paper not only aggregates existing knowledge, but also assesses the applicability and implications of current technologies and theories when generating process models with the assistance of interactive chatbots. The outcome is a foundational compendium for researchers and practitioners interested in exploring this innovative intersection, providing a springboard for future research and development in this promising area.
Quality-Driven Design of Pandan-Flavored Sponge Cake: Unraveling the Role of Thermal Processing on Typical Pandan Aroma
Authors: Chen Xiao , Cao Ying , Lan Weijie , Gu Zixuan , He Wenjia , He Jianfei , Zhao Liyan
Published: September 2024

Pandan (Pandanus amaryllifolius Roxb.) has been used in the production of bakery goods either as a functional ingredient or a natural flavoring that, when roasted, exerts a fragrant rice-like aroma and an attractive green color. This study elucidated the typical aroma compounds from pandan leaves and explored the influence of thermal treatments on their aroma profiles using GC-O-MS, E-nose, and GC-IMS analyses. The effects of formulation and baking conditions on the qualities of pandan-flavored sponge cake were comprehensively evaluated through a holistic approach covering several aspects including cake batter gravity, color, texture, and sensory characteristics. The baking treatment introduced more types of pleasant aromas (9 aromas vs. 17 aromas) and increased the odor intensities of the original volatile compounds, especially for the roasted and steamed rice-like odors. The increased amount of pandan flavoring reshaped the color of the cake crumb (especially for the L* and a* spaces) and significantly decreased the hardness (3.87 N to 1.01 N), gumminess (3.81 N to 0.67 N), and chewiness (13.22 mJ to 4.56 mJ) of the sponge cake. The perceived intensities of bitterness and sweetness can be adjusted by modulating the levels of 2-phenylethanol, 2-methyl-1-butanol, hexyl alcohol, and decanal, along with the total alcohols and aldehydes, due to their significant correlations revealed by correlation heatmap analyses.
Effect of Mixed Probiotics on Alleviating H1N1 Influenza Infection and Regulating Gut Microbiota
Authors: Wang Hongchao , Zhao Yuhao , Pei Zhangming , Zhao Jianxin , Zhang Pinghu , Zhang Xinyue , Zhang Zhijian , Lu Wenwei
Published: September 2024

Influenza and other respiratory infections cause annual epidemics worldwide, with high incidence and mortality rates reported among immunocompromised infants and elderly individuals. Probiotics can modulate the immune system through their bacterial compositions and metabolites, affecting influenza infections and effectively responding to viral mutations. Therefore, we evaluated the anti-influenza effects of mixed probiotics administered orally before and after influenza infection. The results showed that the mixed probiotics consisting of Lacticaseibacillus rhamnosus CCFM1279, Limosilactobacillus reuteri CCFM1145, and Lacticaseibacillus casei CCFM1127 inhibited viral replication and reduced lung inflammatory damage against influenza. In addition, the mixed-probiotics treatment activated the systemic immune response of the host. The gut microbiota analysis revealed a notable increase in the abundance of Alistipes and Rikenella following mixed-probiotic supplementation. The metabolomic analysis indicated a significant increase in adenosine levels, which was positively correlated with the abundance of Parvibacter. These findings highlight the effectiveness of mixed probiotics in fighting influenza viruses and suggest that certain gut microbiota and their metabolites may play a significant role in influencing the outcomes of influenza infections.
Locust Bean Gum/κ-Carrageenan Film Containing Blueberry or Beetroot Extracts as Intelligent Films to Monitoring Hake (Merluccius merluccius) Freshness
Authors: Faria Carla S. V. , Vieira Jorge M. , Vicente António A. , Martins Joana T.
Published: September 2024

The main goal of this work was to develop bio-based and ecofriendly intelligent films as freshness indicators to monitor European hake (Merluccius merluccius) quality during storage by using a visual, non-destructive, and real-time technique. Locust bean gum (LBG)/κ-carrageenan (Car) films incorporating blueberry extract (BLE) or beetroot extract (BEE) were developed and their effectiveness to detect hake deterioration during 7 days of storage at 4 °C was evaluated. A visible color response from pink to blue was observed on the BLE films at the end of hake storage, which correlated with the hake deterioration profile, namely an increase in pH values (from 6.60 ± 0.04 to 8.02 ± 0.03), total viable count (TVC, from 4.61 ± 0.36 to 8.61 ± 0.21 log CFU/g), and total volatile basic nitrogen content (TVB-N, from 10.21 ± 1.97 to 66.78 ± 4.81 mg/100 g) beyond the spoilage threshold. The results of this study are very promising, since it was possible to develop a new effective intelligent bio-based responsive indicator film incorporating natural dye BLE, which has the potential to contribute to food waste reduction and improve food safety by detecting the hake freshness status.
Metabolite Profiling and Identification of Sweet/Bitter Taste Compounds in the Growth of Cyclocarya Paliurus Leaves Using Multiplatform Metabolomics
Authors: Chen Liang , Lu Dai , Wan Yuxi , Zou Yaqian , Zhang Ruiyi , Zhou Tao , Long Bin , Zhu Kangming , Wang Wei , Tian Xing
Published: September 2024

Cyclocarya paliurus tea, also known as “sweet tea”, an herbal tea with Cyclocarya paliurus leaves as raw material, is famous for its unique nutritional benefits and flavor. However, due to the unique “bittersweet” of Cyclocarya paliurus tea, it is still unable to fully satisfy consumers’ high-quality taste experience and satisfaction. Therefore, this study aimed to explore metabolites in Cyclocarya paliurus leaves during their growth period, particularly composition and variation of sweet and bitter taste compounds, by combining multi-platform metabolomics analysis with an electronic tongue system and molecular docking simulation technology. The results indicated that there were significant differences in the contents of total phenols, flavonoids, polysaccharides, and saponins in C. paliurus leaves in different growing months. A total of 575 secondary metabolites were identified as potential active metabolites related to sweet/bitter taste using nontargeted metabolomics based on UHPLC-MS/MS analysis. Moreover, molecular docking technology was utilized to study interactions between the candidate metabolites and the sweet receptors T1R2/T1R3 and the bitter receptors T2R4/T2R14. Six key compounds with high sweetness and low bitterness were successfully identified by using computational simulation analysis, including cis-anethole, gluconic acid, beta-D-Sedoheptulose, asparagine, proline, and citrulline, which may serve as candidates for taste modification in Cyclocarya paliurus leaves. These findings provide a new perspective for understanding the sweet and bitter taste characteristics that contribute to the distinctive sensory quality of Cyclocarya paliurus leaves.
Physicochemical Characterization, Rheological Properties, and Antimicrobial Activity of Sodium Alginate-Pink Pepper Essential Oil (PPEO) Nanoemulsions
Authors: Lima Mariah Almeida , Carusi Juliana , Rocha Liliana de Oliveira , Tonon Renata Valeriano , Cunha Rosiane Lopes , Rosenthal Amauri
Published: September 2024

Essential oils (EOs) have antimicrobial properties, but their low solubility in water and strong flavor pose challenges for direct incorporation into food, as they can negatively impact organoleptic properties. To overcome these issues, strategies such as oil-in-water (O/W) nanoemulsions have been developed to improve EO dispersion and protection while enhancing antimicrobial efficacy. The objective of this study was to create sodium alginate-pink pepper essential oil (PPEO) nanoemulsions using microfluidization. Various formulations were assessed for physicochemical, physical, and antimicrobial properties to evaluate their potential in food applications. The microfluidized emulsions and nanoemulsions had droplet sizes ranging from 160 to 443 nm, polydispersity index (PdI) ranging from 0.273 to 0.638, and zeta potential (ζ) ranging from −45.2 to 66.3 mV. The nanoemulsions exhibited Newtonian behavior and remarkable stability after 20 days of storage. Antimicrobial testing revealed effectiveness against Staphylococcus aureus and Listeria monocytogenes, with minimum inhibitory concentrations (MIC) of 200 µg/mL for both microorganisms and minimum bactericidal concentrations (MBC) of 800 µg/mL and 400 µg/mL, respectively, proving that encapsulation of PPEO in nanoemulsions significantly increased its antibacterial activity. These results present the possibility of using PPEO nanoemulsions as a more effective natural alternative to synthetic preservatives in food systems.
Nutritional Consequences of Celiac Disease and Gluten-Free Diet
Authors: Bianchi Paola Ilaria , Aronico Nicola , Santacroce Giovanni , Broglio Giacomo , Lenti Marco Vincenzo , Di Sabatino Antonio
Published: September 2024

Celiac disease is an immune-mediated condition triggered by gluten ingestion in genetically predisposed individuals. The global prevalence of celiac disease is significant, affecting approximately 1.4% of women and 0.7% of men, with incidence rates of 17.4 and 7.8 per 100,000 person-years, respectively. The clinical presentation of celiac disease may range from overt diarrhea and malabsorption to more subtle features such as nutritional deficiencies and extraintestinal manifestations. It is the most common cause of global malabsorption in Western countries. A life-long gluten-free diet is the only available treatment for celiac disease. Moreover, a gluten-free diet is often adopted by individuals without celiac disease, either to address non-celiac gluten sensitivity or for other reasons. This review aims to explore the current understandings of the nutritional consequences of untreated celiac disease and the impact of the gluten-free diet itself. Physicians and dietitians specializing in celiac disease should focus on providing a well-rounded nutritional scheme to address deficiencies caused by the disease and prevent the instauration of new nutritional imbalances.
Editorial on the Special Issue “Genetic and Molecular Basis of Inherited Disorders”
Authors: Iuliano Rodolfo , Paduano Francesco
Published: September 2024

This Special Issue of Genes, titled “Genetic and Molecular Basis of Inherited Disorders”, presents a collection of pioneering research articles that advance our understanding of the genetic mechanisms underlying various hereditary diseases. The studies employ cutting-edge genomic techniques, including next-generation sequencing and genome-wide association studies, to elucidate novel genetic variants and their functional implications. Key investigations span a diverse range of conditions, from congenital idiopathic nystagmus and hereditary hearing loss to familial hypercholesterolemia and rare cancer predisposition syndromes. Notable findings include the identification of new gene–disease associations in congenital anomalies of the kidney and urinary tract, the discovery of large genomic rearrangements in breast cancer susceptibility, and insights into the genetic basis of pigmentary traits and associated disease risks. This Special Issue also highlights the significance of copy number variations and rare structural variants in disease pathogenesis. Collectively, these studies underscore the complexity of genetic variation in inherited disorders and demonstrate the critical role of integrating advanced genetic analyses with clinical practice to enhance diagnostic precision and develop targeted therapeutic approaches.
Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori
Authors: Yu Yang , Li Tian , Chen Ping
Published: September 2024

Background/Objectives: The gene-encoding farnesyl diphosphate O-methyltransferase 6 (FAMeT 6) is a member of the farnesyl diphosphate O-methyltransferase family. Our previous studies have demonstrated its influence on juvenile hormone levels in third instar silkworm larvae. Methods: we utilized transcriptomic and metabolomic techniques to investigate the changes in third instar larvae at 0, 12, and 24 h following BmFAMeT6 overexpression. Results: (1) The differentially expressed homologous genes were enriched in detoxification-related pathways at all three time points. (2) Transcription factor analysis of DEGs indicated a predominant presence of ZF-C2H2. (3) The metabolite-related network suggested that BmFAMeT6 may influence the metabolism of silkworm larvae through the ABC transporters, purine metabolism, and tyrosine metabolism pathways. (4) The differential gene count, differential metabolite count, and types of metabolites at the three time points indicated a shift in the regulatory focus within the larvae as time progresses, with the inflection point of regulation occurring at the third instar larval stage, 12 h. Conclusion: In summary, our research indicates that the regulatory role of BmFAMeT6 occurs within the context of the domestic silkworm’s own growth and development regulation.
YELLOW LEAF AND DWARF 7, Encoding a Novel Ankyrin Domain-Containing Protein, Affects Chloroplast Development in Rice
Authors: Cui Yongtao , Song Jian , Tang Liqun , Wang Jianjun
Published: September 2024

Background: The proper development of grana and stroma within chloroplasts is critical for plant vitality and crop yield in rice and other cereals. While the molecular mechanisms underpinning these processes are known, the genetic networks governing them require further exploration. Methods and Results: In this study, we characterize a novel rice mutant termed yellow leaf and dwarf 7 (yld7), which presents with yellow, lesion-like leaves and a dwarf growth habit. The yld7 mutant shows reduced photosynthetic activity, lower chlorophyll content, and abnormal chloroplast structure. Transmission electron microscopy (TEM) analysis revealed defective grana stacking in yld7 chloroplasts. Additionally, yld7 plants accumulate high levels of hydrogen peroxide (H2O2) and exhibit an up-regulation of senescence-associated genes, leading to accelerated cell death. Map-based cloning identified a C-to-T mutation in the LOC_Os07g33660 gene, encoding the YLD7 protein, which is a novel ankyrin domain-containing protein localized to the chloroplast. Immunoblot analysis of four LHCI proteins indicated that the YLD7 protein plays an important role in the normal biogenesis of chloroplast stroma and grana, directly affecting leaf senescence and overall plant stature. Conclusions: This study emphasizes the significance of YLD7 in the intricate molecular mechanisms that regulate the structural integrity of chloroplasts and the senescence of leaves, thus providing valuable implications for the enhancement of rice breeding strategies and cultivation.
Emotional Intelligence as Critical Competence in Nurses’ Work Performance: A Cross-Sectional Study
Authors: Galanis Petros , Katsiroumpa Aglaia , Moisoglou Ioannis , Derizioti Konstantina , Gallos Parisis , Kalogeropoulou Maria , Papanikolaou Vasiliki
Published: September 2024

Background/Objectives: Emotional intelligence may help nurses to cope with demanding work environments where the need to improve the quality and safety of the care provided, as well as the care of the chronically ill, prevails. Although it is well known that emotional intelligence is positively related to work performance, the literature on nurses is limited. The aim of our study was to examine the impact of emotional intelligence on work performance in a sample of nurses in Greece. Methods: We conducted a cross-sectional study with 318 nurses. We collected data from a convenience sample of nurses during January 2024. Since we conducted an online survey through social media, our sample could not be representative of all nurses in Greece. For instance, older nurses may be underrepresented in our study due to limited access on social media. We measured emotional intelligence with the Trait Emotional Intelligence Questionnaire-Short Form (TEIQue-SF) and work performance with the Individual Work Performance Questionnaire (IWPQ). We created multivariable linear regression models adjusted for sex, age, educational level, and work experience. We followed the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. Results: We found that the four streams of emotional intelligence (i.e., well-being, self-control, emotionality, sociability) increased nurses’ work performance. In particular, we found a positive relationship between well-being and task performance (adjusted beta = 0.210, 95% CI = 0.140 to 0.281, p-value < 0.001) and contextual performance (adjusted beta = 0.135, 95% CI = 0.050 to 0.221, p-value = 0.002). Similarly, there was a positive relationship between self-control and task performance (adjusted beta = 0.136, 95% CI = 0.030 to 0.241, p-value = 0.012). Additionally, sociability increased task performance (adjusted beta = 0.223, 95% CI = 0.151 to 0.295, p-value < 0.001) and contextual performance (adjusted beta = 0.198, 95% CI = 0.111 to 0.286, p-value < 0.001). Moreover, emotionality (adjusted beta = −0.198, 95% CI = −0.319 to −0.076, p-value = 0.002) and sociability (adjusted beta = −0.133, 95% CI = −0.221 to −0.044, p-value = 0.003) reduced counterproductive work behavior. Conclusions: Our multivariable models identified a positive impact of emotional intelligence on nurses’ work performance. Nurse managers and healthcare organizations should adopt appropriate interventions to improve nurses’ emotional intelligence. Enhancing emotional intelligence among nurses can improve work performance and, thus, healthcare outcomes. Moreover, higher levels of emotional intelligence may empower nurses’ compassion and resilience, fostering a supportive work environment. In this context, the well-being of both nurses and patients may improve.
Switching from Cigarettes to Heated Tobacco Products in Japan—Potential Impact on Health Outcomes and Associated Health Care Costs
Authors: Mahlich Joerg , Kamae Isao
Published: September 2024

Background: Japan’s rising health expenditure, driven by an aging population, coincides with growing demands for increased spending. Reducing smoking-related costs could alleviate the burden on the health care system. Despite efforts to promote smoking cessation, success has been limited, indicating a need for strategies beyond cessation. Methods: Using a status quo simulation based on hospital resource data from the Japanese Ministry of Health, Labor, and Welfare, we examine the impact of heated tobacco products (HTPs) on the prevalence of four smoking-attributable diseases (chronic obstructive pulmonary disease, ischemic heart disease, stroke, and lung cancer) and the related direct health care costs. The baseline scenario assumes a 50% switch from combustible cigarettes to HTPs, with a 70% risk reduction. A sensitivity analysis was conducted to assess the effects of parameter variations. Results: If 50% of smokers replaced combustible tobacco products with HTPs, 12 million patients could be averted equivalent to JPY 454 billion in health care savings. Prefectures located in the north and south of Japan would benefit the most. Conclusions: Considering the heterogeneous prevalence rates, a one-size-fits-all tobacco control approach is ineffective. Japan should prioritize cost-efficient measures that promote public health and economic benefits. Encouraging smokers to switch to reduced-risk products, raising awareness of health risks, and adopting a harm-based taxation model can drive positive change. Public–private partnerships can further enhance harm reduction efforts. With a combination of tax reforms, revised regulations, collaborations, and ongoing research, Japan can create a more effective and comprehensive approach to tobacco control.
Trends and Patterns of ICU E-Referrals in Saudi Arabia during 2020–2021: Results from the National Saudi Medical Appointments and Referrals Centre
Authors: Alharbi Abdullah A. , Aljerian Nawfal A. , Alghamdi Hani A. , Binhotan Meshary S. , Alsultan Ali K. , Arafat Mohammed S. , Aldhabib Abdulrahman , Aloqayli Ahmed I. , Alwahbi Eid B. , Muaddi Mohammed A. , Alabdulaali Mohammed K.
Published: September 2024

Background/Objectives: Intensive care unit (ICU) e-referrals are an important indicator for exploring potential deficiencies in critical care resources. This study aimed to examine the epidemiology and patterns of ICU e-referrals across all regions of Saudi Arabia during the COVID-19 pandemic using routinely collected data from April 2020 to December 2021. Methods: This descriptive epidemiological study analyzed data from the Saudi Medical Appointments and Referrals Centre (SMARC). This study reveals novel regional ICU e-referral patterns for critical cases using national unique digital health data, adding insights beyond the existing literature. Variables included age, sex, referral timing, reason, specialty, and region of origin. Descriptive statistics and mapping of administrative areas were performed based on e-referral request rates per 10,000 population. Results: During the study period, 36,619 patients had ICU e-referral requests. The mean age was 54.28 years, with males constituting 64.81% of requests. Out-of-scope e-referrals comprised 71.44% of requests. Referrals related to medical specialties, such as cardiology and pulmonology, were the most common (62.48%). Referral patterns showed peaks in July–August 2020 and May 2021. The Northern Border and Albaha areas had the highest request rates per population, potentially reflecting a higher proportion of severe cases requiring ICU-level care compared to other regions. Conclusions: The temporal pattern and geographic distribution of ICU e-referrals mirrored previously reported critical COVID-19 cases in Saudi Arabia. Preventive measures and vaccination programs contributed to a significant decline in ICU e-referral requests, suggesting a positive impact on controlling severe COVID-19 cases. Population-adjusted analysis revealed regional disparities, highlighting the importance of considering population size in healthcare resource management and policy.
Assessing the Relationships between Physical Activity, a Healthy Life, and Personal Happiness in European Union Countries
Authors: Mangra Gabriel Ioan , Mangra Mădălina Giorgiana , Bocean Claudiu George , Vărzaru Anca Antoaneta
Published: September 2024

Background: Maintaining a physically active lifestyle is a determinant factor of a healthy life and personal happiness. Meanwhile, physical inactivity remains a significant issue, resulting in negative consequences for public health. Objectives: This paper investigates the relationships between physical activity, physical inactivity, a healthy life, life expectancy, and personal happiness in European Union (EU) countries. Methods: This empirical study uses an artificial neural network and cluster analysis to analyze and interpret data from 27 EU countries. Artificial neural network analysis enables the assessment of the relationships between physical activity and inactivity, a healthy life, and personal happiness, while cluster analysis identifies groups of EU countries based on physical activity, healthy life, and personal happiness indicators. Results: The results show significant positive links between physical activity and improvements in healthy living and personal happiness. Conclusions: This study highlights considerable variations among EU countries regarding the levels of physical activity, healthy living, and personal happiness, emphasizing the importance of promoting physical activity to enhance public health and overall well-being. The findings suggest the need to develop customized policies that address country-specific factors and promote an active lifestyle.
Terrorism Risk Assessment for Historic Urban Open Areas
Authors: Cantatore Elena , Quagliarini Enrico , Fatiguso Fabio
Published: September 2024

Making cities resilient and secure remains a central goal in urban policy strategies, where established methods, technologies, and best experiences are applied or replicated when the knowledge of a threat is already well established. The scientific community and specialized bodies are invited to comprehend and evaluate disastrous events that are still not well explored to broaden the concept of resilient cities. Among these, terrorism in the European-built environment remains an underexplored topic, despite various studies assessing its economic, social, and political dimensions, exploring the radicalist matrix, or examining the post-effects of high-impact disastrous events. Within this framework, this work presents an algorithm for the risk assessment of historic urban open areas (uOAs) in Europe, combining theories of the terrorism phenomenon, the normative experiences, and the phenomenological results of violent acts in uOAs. Specifically, the algorithm is determined by studying physical qualities/properties and elements that usually feature the uOAs, using a limited set of descriptors. The descriptors and their formulation are set starting from their qualification, in compliance with the risk determinant (Hazard, Vulnerability, and Exposure), and discussed starting from participatory methods (Delphi and AHP). The algorithm is finally applied to Italian historic squares, testing the mathematical approach, verifying theories of the phenomenon, and setting up a comprehensive three-dimensional risk matrix for both soft and hard targets. This latest constitutes an operative tool to assess the investigated built environment exposed to terrorist threats aimed at developing more detailed mitigative strategies.
Assessment of Long-Term Changes in Knowledge and Attitudes of Household Contacts of COVID-19 Cases in Northern Spain
Authors: Vera-Punzano Noelia , Bullón-Vela Vanessa , Miret Carme , Pardos-Plaza Jéssica , García Cenoz Manuel , Godoy Pere , Castilla Jesús , Domínguez Àngela , Toledo Diana , Martínez-Baz Iván
Published: September 2024

This study aims to describe the long-term changes in the knowledge of, and attitudes towards, COVID-19 and its preventive measures in northern Spain. A telephonic survey was performed among household contacts of COVID-19 cases in Catalonia and Navarre between May 2022 and December 2023. Knowledge and attitudes were assessed through 12 questions using a Likert scale, and responses were grouped as correct or incorrect. The change from baseline to the 6-month follow-up was evaluated with the absolute difference (AD) using the proportion of correct answers. At baseline, 299 subjects were contacted, of whom 63.2% (189) completed the 6-month follow-up. Correct knowledge of transmission (>85%) and the use of preventive measures (>92%) were observed at baseline and maintained over time. The attitudes towards face mask use remained adequate over the course of six months (>79%). However, attitudes regarding the use of face masks indoors (AD = −16.4%; p < 0.001) and those who thought that COVID-19 had a negative impact on their lives (AD = −16.5%; p < 0.001) decreased after 6 months. In the post-acute phase of the pandemic, household contacts maintained the correct level of knowledge towards COVID-19, while some attitudes decreased. These results should serve as a guide for health policy makers in decision-making in case of a new increase in the incidence of SARS-CoV-2.
A Disposable Diaper Collection Project in Langa, Cape Town, South Africa: A Pilot Study
Authors: Schenck Catherina , Tyrrell Hugh , Grobler Lizette , Niyobuhungiro Rissa , Kimani Alexander
Published: September 2024

In developing countries, there is currently no established waste management plan that includes resource recovery from used disposable diapers (DDs) apart from incineration and landfilling. In low-income areas with limited storage space, the complex composition and odour of used DDs make it impossible to manage properly if not supported by effective waste management systems. In the absence of effective waste management, DDs are dumped in open spaces, burned or buried. These actions pose threats to the safety and health of humans, animals and the environment. Separation and collection of DDs are critical preliminary steps to landfilling, recycling or beneficiation. In this article, we describe a case study of two pilot collection projects in Langa township in Cape Town, South Africa, to determine whether and how a source-separated collection system can work in low-income, resource-constrained areas. The lessons learned highlighted the following: The eagerness of parents to participate for the benefit of their own and their children’s health; the complementarity of the two pilot collection models to serve the needs of the community; the important role non-government organisations play in the implementation of waste management projects; the significance of the possible job creation opportunities and the unintended benefits of enhancing social cohesion. The financial sustainability of these projects needs further exploration.
Macrophages in the Context of Muscle Regeneration and Duchenne Muscular Dystrophy
Authors: Hernandez-Torres Francisco , Matias-Valiente Lidia , Alzas-Gomez Virginia , Aranega Amelia Eva
Published: September 2024

Macrophages are essential to muscle regeneration, as they regulate inflammation, carry out phagocytosis, and facilitate tissue repair. These cells exhibit phenotypic switching from pro-inflammatory (M1) to anti-inflammatory (M2) states during muscle repair, influencing myoblast proliferation, differentiation, and myofiber formation. In Duchenne Muscular Dystrophy (DMD), asynchronous muscle injuries disrupt the normal temporal stages of regeneration, leading to fibrosis and failed regeneration. Altered macrophage activity is associated with DMD progression and physiopathology. Gaining insight into the intricate relationship between macrophages and muscle cells is crucial for creating effective therapies aimed at treating this muscle disorder. This review explores the dynamic functions of macrophages in muscle regeneration and their implications in DMD.
Adaptive Differences in Cellular and Behavioral Responses to Circadian Disruption between C57BL/6 and BALB/c Strains
Authors: Ma Changxiao , Li Haonan , Li Wenyu , Yang Guangrui , Chen Lihong
Published: September 2024

The regulation of the mammalian circadian clock is largely dependent on heredity. In model animals for circadian rhythm studies, C57BL/6 and BALB/c mice exhibit considerable differences in their adaptation to circadian disruption, yet deeper comparisons remain unexplored. Here, we have established embryonic fibroblast cells derived from C57BL/6 mice (MEF) and BALB/c (BALB/3T3) mice, which have been transfected with the Bmal1 promoter-driven luciferase (Bmal1-Luc) reporter gene. Next, dexamethasone was applied for various cyclic stimulations, which revealed that Bmal1 bioluminescence of MEF cells was entrained to 24 to 26 h cycles, whereas BALB/3T3 cells have a wider range (22 to 28 h) with lower amplitudes. Behaviorally, BALB/c mice swiftly adapted to a 6-h advance light/dark cycle, unlike C57BL/6 mice. Furthermore, we found the expression of the circadian rhythm gene Npas2 in BALB/c mice is significantly lower than that in C57BL/6 mice. This observation is consistent with the differentially expressed genes (DEGs) in the intestine and lung tissues of C57BL/6 and BALB/c mice, based on the RNA-seq datasets downloaded from the Gene Expression Omnibus (GEO). In summary, our study uncovers that BALB/c mice possess greater resilience in circadian rhythm than C57BL/6 mice, both cellular and behaviorally, identifying potential genes underlying this difference.
A Narrative Review of the Evolution of Diagnostic Techniques and Treatment Strategies for Acral Lentiginous Melanoma
Authors: Choi Myoung Eun , Choi Eun Ji , Jung Joon Min , Lee Woo Jin , Jo Yoon-Seo , Won Chong Hyun
Published: September 2024

Acral melanoma (AM) is a subtype of cutaneous melanoma located on the palms, soles, and nails. The pathogenesis of AM involves mechanical stimulation and characteristic tumor-promoting mutations, such as those in the KIT proto-oncogene. Dermoscopy is useful for diagnosing AM, which is characterized by parallel ridge patterns and irregular diffuse pigmentation. Although histopathological confirmation is the gold standard for diagnosing AM, lesions showing minimal histopathological changes should be considered early-stage AM if they clinically resemble it. Recently, immunohistochemical staining of preferentially expressed antigen in melanoma has been recognized as a useful method to distinguish benign from malignant melanocytic tumors. Research reveals that AM is associated with an immunosuppressive microenvironment characterized by increased numbers of M2 macrophages and regulatory T cells, alongside a decreased number of tumor-infiltrating lymphocytes. Mohs micrographic surgery or digit-sparing wide local excision has been explored to improve quality of life and replace wide local excision or proximal amputation. AM has a worse prognosis than other subtypes, even in the early stages, indicating its inherent aggressiveness.
Biotransformation of Xanthohumol by Entomopathogenic Filamentous Fungi
Authors: Łój Daniel , Janeczko Tomasz , Bartmańska Agnieszka , Huszcza Ewa , Tronina Tomasz
Published: September 2024

Xanthohumol (1) is a major prenylated flavonoid in hops (Humulus lupulus L.) which exhibits a broad spectrum of health-promoting and therapeutic activities, including anti-inflammatory, antioxidant, antimicrobial, and anticancer effects. However, due to its lipophilic nature, it is poorly soluble in water and barely absorbed from the gastrointestinal tract, which greatly limits its therapeutic potential. One method of increasing the solubility of active compounds is their conjugation to polar molecules, such as sugars. Sugar moiety introduced into the flavonoid molecule significantly increases polarity, which results in better water solubility and often leads to greater bioavailability. Entomopathogenic fungi are well known for their ability to catalyze O-glycosylation reactions. Therefore, we investigated the ability of selected entomopathogenic filamentous fungi to biotransform xanthohumol (1). As a result of the experiments, one aglycone (2) and five glycosides (3–7) were obtained. The obtained (2″E)-4″-hydroxyxanthohumol 4′-O-β-D-(4‴-O-methyl)-glucopyranoside (5) has never been described in the literature so far. Interestingly, in addition to the expected glycosylation reactions, the tested fungi also catalyzed chalcone–flavanone cyclization reactions, which demonstrates chalcone isomerase-like activity, an enzyme typically found in plants. All these findings undoubtedly indicate that entomopathogenic filamentous fungi are still an underexploited pool of novel enzymes.
Comparative Metabolome and Transcriptome Analysis Reveals the Defense Mechanism of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) against Plasmodiophora brassicae Infection
Authors: Wei Xiaochun , Du Yingyi , Zhang Wenjing , Zhao Yanyan , Yang Shuangjuan , Su Henan , Wang Zhiyong , Wei Fang , Tian Baoming , Yang Haohui , Zhang Xiaowei , Yuan Yuxiang
Published: September 2024

Chinese cabbage (Brassica rapa L. ssp. pekinensis) ranks among the most cultivated and consumed vegetables in China. A major threat to its production is Plasmodiophora brassicae, which causes large root tumors, obstructing nutrient and water absorption and resulting in plant withering. This study used a widely targeted metabolome technique to identify resistance-related metabolites in resistant (DH40R) and susceptible (DH199S) Chinese cabbage varieties after inoculation with P. brassicae. This study analyzed disease-related metabolites during different periods, identifying 257 metabolites linked to resistance, enriched in the phenylpropanoid biosynthesis pathway, and 248 metabolites linked to susceptibility, enriched in the arachidonic acid metabolism pathway. Key metabolites and genes in the phenylpropanoid pathway were upregulated at 5 days post-inoculation (DPI), suggesting their role in disease resistance. In the arachidonic acid pathway, linoleic acid and gamma-linolenic acid were upregulated at 5 and 22 DPI in resistant plants, while arachidonic acid was upregulated at 22 DPI in susceptible plants, leading to the conclusion that arachidonic acid may be a response substance in susceptible plants after inoculation. Many genes enriched in these pathways were differentially expressed in DH40R and DH199S. The research provided insights into the defense mechanisms of Chinese cabbage against P. brassicae through combined metabolome and transcriptome analysis.
Use of Bacterial Toxin–Antitoxin Systems as Biotechnological Tools in Plants
Authors: Rodamilans Bernardo , Cheng Xiaofei , Simón-Mateo Carmen , García Juan Antonio
Published: September 2024

Toxin–antitoxin (TA) systems in bacteria are key regulators of the cell cycle and can activate a death response under stress conditions. Like other bacterial elements, TA modules have been widely exploited for biotechnological purposes in diverse applications, such as molecular cloning and anti-cancer therapies. However, their use in plants has been limited, leaving room for the development of new approaches. In this study, we examined two TA systems previously tested in plants, MazEF and YefM-YoeB, and identified interesting differences between them, likely related to their modes of action. We engineered modifications to these specific modules to transform them into molecular switches that can be activated by a protease, inducing necrosis in the plant cells where they are expressed. Finally, we demonstrated the antiviral potential of the modified TA modules by using, as a proof-of-concept, the potyvirus plum pox virus as an activator of the death phenotype.
Molecular Markers for Analyses of Genetic Diversity within the Anastrepha fraterculus Complex with Emphasis on Argentine Populations
Authors: Gomulski Ludvik M. , Vera María Teresa , Lanzavecchia Silvia B. , Piccinno Riccardo , Fiorenza Giulia , De Luca Daniel , Carrizo Beatriz N. , Bouvet Juan Pedro R. , Viana Valeria A. , Cárceres Carlos , Enkerlin Walther , Malacrida Anna R. , Gasperi Giuliano
Published: September 2024

The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi.