bluuebunny/crossref_metadata_2025 huggingface dataset:
A Robust Feedback Path Tracking Control Algorithm for an Indoor Carrier Robot Considering Energy Optimization
Authors: Wang Yina , Xiong Wenqiu , Yang Junyou , Jiang Yinlai , Wang Shuoyu
Published: May 2019

This work develops an indoor carrier robot for people with disabilities, where the precise tracking of designated route is crucial. The parameter uncertainties and disturbances of the robot impose significant challenges for tracking. The present paper first investigates the dynamic of mechanical structure and modeling of actuator motors and constructs a new dynamic model by considering all main parameter uncertainties and disturbances. A novel robust feedback tracking controller considering both the optimization of path tracking and the minimization of the power consumption energy is proposed. It is proved that the tracking errors e and e ˙ satisfy a H∞ performance indicator while the energy consumption is minimum. A simulation example was performed and the results show that this novel algorithm can effectively reduce the tracking error from 0.2 m to 0.006 m while guaranteeing the minimum energy consumption. Furthermore, the effectiveness of the proposed method was validated by experiment compared with the non-robust one.
Co-Optimized Analysis and Design of Electric and Natural Gas Infrastructures
Authors: Lemos-Cano Santiago , McCalley James
Published: May 2019

This paper proposes and implements a long-term deterministic capacity expansion model for the co-optimization of electric and natural gas infrastructures. It determines the required investments in generation units, transmission lines and pipelines for meeting future demands, while representing electricity and natural gas flows using DC Power Flow and Weymouth equations, respectively. A Mixed Integer Nonlinear Programming (MINLP) problem is developed, from which a linearized version is derived. A 26 node integrated gas-electric system for the Eastern Region of the United States is used to demonstrate the model’s capabilities. Results show that the model provides an accurate operational representation of the integrated system, and, therefore, enhances the expansion planning process.
Unsteady Flow Numerical Simulations on Internal Energy Dissipation for a Low-Head Centrifugal Pump at Part-Load Operating Conditions
Authors: Zhao Xiaoran , Luo Yongyao , Wang Zhengwei , Xiao Yexiang , Avellan François
Published: May 2019

Dredge pumps are usually operated at part-load conditions, in which the low-solidity centrifugal impeller could experience large internal energy dissipation, related to flow separation and vortices. In this study, SST k-ω and SAS-SST turbulence models were used, in steady and unsteady simulations, for a low-head centrifugal pump with a three-bladed impeller. The main focus of the present work was to investigate the internal energy dissipation in rotating an impeller at part-load operating conditions, related to flow separation and stall. The unsteady nature of these operating conditions was investigated. Performance experiments and transient wall pressure measurements were conducted for validation. A methodology for internal energy dissipation analysis has been proposed; and the unsteady pressure fluctuations were analyzed in the rotating impeller. The internal power losses in the volute and the impeller were mostly found in the centrifugal pump. The rotating stall phenomenon occurred with flow separation and detachment at the part-load operating condition, leading to a dissipation of the internal energy in the impeller. The rotating impeller experienced pressure fluctuations with low frequencies, at part-load operating conditions, while in the design operating condition only experienced rotating frequency.
Effect of Actual Gas Turbine Operating Conditions on Mist/Steam Cooling Performance in a Ribbed Passage
Authors: Gong Jianying , Gao Tieyu , Zeng Junxiong , Hou Jianqiang , Li Zhen
Published: May 2019

This study numerically examines the effect of actual gas turbine operating conditions on heat transfer characteristics in a ribbed passage with mist/steam cooling. A 60° ribbed passage with aspect ratio of 1/1 was investigated at Reynolds number of 300,000, and steam cooling was used to provide a contrast. Three main factors were considered: coolant temperature, operating pressure, and wall heat flux density. The heat transfer enhancement mechanism of mist/steam cooling was explored, and the results showed that the heat transfer performance of mist/steam cooling was superior to steam cooling. When the coolant temperature varied from 300 to 500 °C, the average Nusselt number of mist/steam cooling decreased by 26.6%, and the heat transfer enhancement ratio dropped from 15% to 10%. As operating pressure increased, the heat transfer performance factor of mist/steam firstly increased and then decreased. At an operating pressure of 1.5 MPa, the heat transfer achieved its optimal performance, and the heat transfer enhancement ratio achieved its maximum value of 15.9%. Larger wall heat flux density provided less heat transfer enhancement. When the heat flux density increased from 100,000 to 300,000 W·m−2, the average Nusselt number of mist/steam cooling decreased by 13.8%, while the heat transfer enhancement ratio decreased from 25.3% to 12.6%.
Accurate Expressions of Mutual Inductance and Their Calculation of Archimedean Spiral Coils
Authors: Liu Shuo , Su Jianhui , Lai Jidong
Published: May 2019

Considering the helicity of Archimedean spiral coils, this paper proposes accurate expressions of mutual inductance and their numerical calculation methods, which can be applied in the wireless power transmission field, etc. Accurate expressions of mutual inductance are deduced respectively for two coils that are coaxial, laterally misaligned, or non-parallel, and numerical calculations are performed using Gaussian integration as well. In the case of coaxial coils, the calculation results are verified by the 3D finite element method (3D FEM) and compared with the results gained by the traditional method that approximates two spiral coils to two clusters of series-connected circular coils ignoring helicity. The comparison of the three methods shows that results achieved by the proposed expression are close to that of 3D FEM, while there is increasing error with the screw pitches of the coils when using the traditional circular coil approximation method. The influence of relative position on the mutual inductance of the two coils is also studied and it is further explained through magnetic field distribution. Finally, the validity of the proposed expressions of mutual inductance is verified by experimental results.
Measured Performance of a Mixed-Use Commercial-Building Ground Source Heat Pump System in Sweden
Authors: Spitler Jeffrey , Gehlin Signhild
Published: May 2019

When the new student center at Stockholm University in Sweden was completed in the fall of 2013 it was thoroughly instrumented. The 6300 m2 four-story building with offices, a restaurant, study lounges, and meeting rooms was designed to be energy efficient with a planned total energy use of 25 kWh/m2/year. Space heating and hot water are provided by a ground source heat pump (GSHP) system consisting of five 40 kW off-the-shelf water-to-water heat pumps connected to 20 boreholes in hard rock, drilled to a depth of 200 m. Space cooling is provided by direct cooling from the boreholes. This paper uses measured performance data from Studenthuset to calculate the actual thermal performance of the GSHP system during one of its early years of operation. Monthly system coefficients-of-performance and coefficients-of-performance for both heating and cooling operation are presented. In the first months of operation, several problems were corrected, leading to improved performance. This paper provides long-term measured system performance data from a recently installed GSHP system, shows how the various system components affect the performance, presents an uncertainty analysis, and describes how some unanticipated consequences of the design may be ameliorated. Seasonal performance factors (SPF) are evaluated based on the SEPEMO (“SEasonal PErformance factor and MOnitoring for heat pump systems”) boundary schema. For heating (“H”), SPFs of 3.7 ± 0.2 and 2.7 ± 0.13 were obtained for boundaries H2 and H3, respectively. For cooling (“C”), a C2 SPF of 27 ± 5 was obtained. Results are compared to measured performance data from 55 GSHP systems serving commercial buildings that are reported in the literature.
A Four-Winding Inductive Filtering Transformer to Enhance Power Quality in a High-Voltage Distribution Network Supplying Nonlinear Loads
Authors: Chen Yuehui , Huang Zhao , Duan Zhenfeng , Fu Pengwu , Zhou Guandong , Luo Longfu
Published: May 2019

This paper solves the problem of reactive power and harmonics compensation in a high-voltage (HV) distribution network supplying nonlinear loads. An inductive filtering (IF) approach where passive filters connect to the filtering winding of a four-winding inductive filtering transformer (FW-IFT) is presented to enhance the power quality of the public grid. This method can not only greatly suppress harmonic currents of the medium and/or low-voltage (LV) side, but also prevent them from flowing into the public grid. The new main circuit topology, where the FW-IFT has specific filtering winding by adopting the ampere-turn balance of the transformer, is presented. On the basis of the structure of the FW-IFT, the magnetic potential balanced equation and inductive filtering technology, its equivalent circuit and mathematical model are established, and the filtering performances are analyzed in detail. Simulation and experimental results rated at SN-10/0.38 of the FW-IFT are presented to prove the efficacy of the comprehensive enhancement of power quality on the grid side.
Volumetric Measurements of Methane-Coal Adsorption and Desorption Isotherms—Effects of Equations of State and Implication for Initial Gas Reserves
Authors: Ekundayo Jamiu M. , Rezaee Reza
Published: May 2019

This study presents the effects of equations of state (EOSs) on methane adsorption capacity, sorption hysteresis and initial gas reserves of a medium volatile bituminous coal. The sorption experiments were performed, at temperatures of 25 °C and 40 °C and up to 7MPa pressure, using a high-pressure volumetric analyzer (HPVA-II). The measured isotherms were parameterized with the modified (three-parameter) Langmuir model. Gas compressibility factors were calculated using six popular equations of state and the results were compared with those obtained using gas compressibility factors from NIST-Refprop® (which implies McCarty and Arp’s EOS for Z-factor of helium and Setzmann and Wagner’s EOS for that of methane). Significant variations were observed in the resulting isotherms and associated model parameters with EOS. Negligible hysteresis was observed with NIST-refprop at both experimental temperatures, with the desorption isotherm being slightly lower than the adsorption isotherm at 25 °C. Compared to NIST-refprop, it was observed that equations of state that gave lower values of Z-factor for methane resulted in “positive hysteresis”, (one in which the desorption isotherm is above the corresponding adsorption curve) and the more negatively deviated the Z-factors are, the bigger the observed hysteresis loop. Conversely, equations of state that gave positively deviated Z-factors of methane relatively produced “negative hysteresis” loops where the desorption isotherms are lower than the corresponding adsorption isotherms. Adsorbed gas accounted for over 90% of the calculated original gas in place (OGIP) and the larger the Langmuir volume, the larger the proportion of OGIP that was adsorbed.
Relationship between Soil Burn Severity in Forest Fires Measured In Situ and through Spectral Indices of Remote Detection
Authors: Sobrino Jose , Llorens Rafael , Fernández Cristina , Fernández-Alonso José , Vega José
Published: May 2019

Forest fires in Galicia have become a serious environmental problem over the years. This is especially the case in the Pontevedra region, where in October 2017 large fires (>500 hectares) burned more than 15,000 Ha. In addition to the area burned being of relevance, it is also very important to know quickly and accurately the different severity degrees that soil has suffered in order to carry out an optimal restoration campaign. In this sense, the use of remote sensing with the Sentinel-2 and Landsat-8 satellites becomes a very useful resource due to the variations that appear in soil after a forest fire (changes in soil cover are noticeably appreciated with spectral information). To calculate these variations, the spectral indices NBR (Normalized Burn Ratio) and NDVI (Normalized Difference Vegetation Index) are used, both before and after the fire and their differences (dNBR and dNDVI, respectively). In addition, as a reference for a correct discrimination between severity degrees, severity data measured in situ after the fire are used to classified at 5 levels of severity and 6 levels of severity. Therefore, this study aims to establish a methodology, which relates remote-sensing data (spectral indices) and severity degrees measured in situ. The R2 statistic and the pixel classification accuracy results show the existing synergy of the Sentinel-2 dNBR index with the 5 severity degrees classification (R2 = 0.74 and 81% of global accuracy) and, for this case, the good applicability of remote sensing in the forest fire field.
Magnetic Field Vector Structure of NGC6946
Authors: Kurahara Kohei , Nakanishi Hiroyuki
Published: May 2019

We studied large-scale magnetic field reversals of a galaxy based on a magnetic vector map of NGC6946. The magnetic vector map was constructed based on the polarization maps in the C and X bands after the determination of the geometrical orientation of a disk with the use of an infrared image and the velocity field, according to the trailing spiral arm assumption. We examined the azimuthal variation of the magnetic vector and found that the magnetic pitch angle changes continually as a function of the azimuthal angle in the inter-arm region. However, the direction of the magnetic field had 180 ∘ jumps at the azimuthal angles of 20 ∘ , 110 ∘ , 140 ∘ , 220 ∘ , 280 ∘ , and 330 ∘ . These reversals seem to be related to the spiral arms since the locations of the jumps are coincident with those of the spiral arms. These six reversals of the magnetic field were seen only in the inner region of NGC6946 whereas four reversals can be identified in the outer region.
Ecohydrological Behaviour of Mountain Beech Forest: Quantification of Stomatal Conductance Using Sap Flow Measurements
Authors: Su Ye , Shao Wei , Vlček Lukáš , Langhammer Jakub
Published: May 2019

In forested regions, transpiration as a main component of evaporation fluxes is important for evaporation partitioning. Physiological behaviours among various vegetation species are quite different. Thus, an accurate estimation of the transpiration rate from a certain tree species needs specific parameterization of stomatal response to multiple environmental conditions. In this study, we chose a 300-m2 beech forest plot located in Vydra basin, the Czech Republic, to investigate the transpiration of beech (Fagus sylvatica) from the middle of the vegetative period to the beginning of the deciduous period, covering 100 days. The sap flow equipment was installed in six trees with varying ages among 32 trees in the plot, and the measurements were used to infer the stomatal conductance. The diurnal pattern of stomatal conductance and the response of stomatal conductance under the multiple environmental conditions were analysed. The results show that the stomatal conductance inferred from sap flow reached the highest at midday but, on some days, there was a significant drop at midday, which might be attributed to the limits of the hydraulic potential of leaves (trees). The response of stomatal conductance showed no pattern with solar radiation and soil moisture, but it did show a clear correlation with the vapour deficit, in particular when explaining the midday drop. The relation to temperature was rather scattered as the measured period was in the moderate climate. The findings highlighted that the parametrization of stress functions based on the typical deciduous forest does not perfectly represent the measured stomatal response of beech. Therefore, measurements of sap flow can assist in better understanding transpiration in newly formed beech stands after bark beetle outbreaks in Central Europe.
Has China’s Pilot Emissions Trading Scheme Influenced the Carbon Intensity of Output?
Authors: Zhang Kangkang , Xu Deyi , Li Shiran , Zhou Na , Xiong Jinhui
Published: May 2019

China launched the pilot construction of the carbon emission trading scheme (ETS) in 2011. The pilots have been running for many years. Does ETS significantly restrain the increase of carbon emission intensity? Based on China’s panel data for provinces and industries, this paper uses the policy assessment method to evaluate the inhibition by ETS of carbon emission intensity. The assessment scope includes six provincial pilots and pilot industries covered by ETS. The results show that ETS has significant suppression of carbon emission intensity only in Beijing and Guangdong. There is no significant impact on the carbon emission intensity of Tianjin, Shanghai, Chongqing, and Hubei. Through the carbon emission intensity inhibition analysis of the industries covered by ETS from Beijing and Chongqing, the results of the production and supply of electric power, steam and hot water, petroleum processing and coking in Beijing have a significant impact on the ETS. Only the smelting and pressing of ferrous metals in Chongqing has a significant impact on the ETS.
Interactions between Ambient Air Particles and Greenness on Cause-specific Mortality in Seven Korean Metropolitan Cities, 2008–2016
Authors: Kim Sera , Kim Honghyok , Lee Jong-Tae
Published: May 2019

This study aims to investigate the association of particulate matter with an aerodynamic diameter smaller than 10 μm (PM10) and greenness with cause-specific mortality and their interactions in seven Korean metropolitan cities. We obtained the annual standardized cause-specific mortality rates, annual mean concentration of PM10, and annual Normalized Difference Vegetation Index (NDVI) for 73 districts for the period 2008–2016. We used negative binomial regression with city-specific random effects to estimate the association of PM10 and greenness with mortality. The models were adjusted for potential confounders and spatial autocorrelation. We also conducted stratified analyses to investigate whether the association between PM10 and mortality differs by the level of greenness. Our findings suggest an increased risk of all causes examined, except respiratory disease mortality, with high levels of PM10 and decreased risk of cardiovascular-related mortality with a high level of greenness. In the stratified analyses, we found interactions between PM10 and greenness, but these interactions in the opposite direction depend on the cause of death. The effects of PM10 on cardiovascular-related mortality were attenuated in greener areas, whereas the effects of PM10 on non-accidental mortality were attenuated in less green areas. Further studies are needed to explore the underlying mechanisms.
Stock Market Analysis: A Review and Taxonomy of Prediction Techniques
Authors: Shah Dev , Isah Haruna , Zulkernine Farhana
Published: May 2019

Stock market prediction has always caught the attention of many analysts and researchers. Popular theories suggest that stock markets are essentially a random walk and it is a fool’s game to try and predict them. Predicting stock prices is a challenging problem in itself because of the number of variables which are involved. In the short term, the market behaves like a voting machine but in the longer term, it acts like a weighing machine and hence there is scope for predicting the market movements for a longer timeframe. Application of machine learning techniques and other algorithms for stock price analysis and forecasting is an area that shows great promise. In this paper, we first provide a concise review of stock markets and taxonomy of stock market prediction methods. We then focus on some of the research achievements in stock analysis and prediction. We discuss technical, fundamental, short- and long-term approaches used for stock analysis. Finally, we present some challenges and research opportunities in this field.
What is the Best Radionuclide for Immuno-PET of Multiple Myeloma? A Comparison Study Between 89Zr- and 64Cu-Labeled Anti-CD138 in a Preclinical Syngeneic Model
Authors: Bailly Clément , Gouard Sébastien , Guérard François , Chalopin Benjamin , Carlier Thomas , Faivre-Chauvet Alain , Remaud-Le Saëc Patricia , Bourgeois Mickaël , Chouin Nicolas , Rbah-Vidal Latifa , Tripier Raphaël , Haddad Ferid , Kraeber-Bodéré Françoise , Bodet-Milin Caroline , Chérel Michel
Published: May 2019

Although positron emission tomography (PET) imaging with 18-Fluorodeoxyglucose (18F-FDG) is a promising technique in multiple myeloma (MM), the development of other radiopharmaceuticals seems relevant. CD138 is currently used as a standard marker for the identification of myeloma cells and could be used in phenotype tumor imaging. In this study, we used an anti-CD138 murine antibody (9E7.4) radiolabeled with copper-64 (64Cu) or zirconium-89 (89Zr) and compared them in a syngeneic mouse model to select the optimal tracers for MM PET imaging. Then, 9E7.4 was conjugated to TE2A-benzyl isothiocyanate (TE2A) and desferrioxamine (DFO) chelators for 64Cu and 89Zr labeling, respectively. 64Cu-TE2A-9E7.4 and 89Zr-DFO-9E7.4 antibodies were evaluated by PET imaging and biodistribution studies in C57BL/KaLwRij mice bearing either 5T33-MM subcutaneous tumors or bone lesions and were compared to 18F-FDG-PET imaging. In biodistribution and PET studies, 64Cu-TE2A-9E7.4 and 89Zr-DFO-9E7.4 displayed comparable good tumor uptake of subcutaneous tumors. On the bone lesions, PET imaging with 64Cu-TE2A-9E7.4 and 89Zr-DFO-9E7.4 showed higher uptake than with 18F-FDG-PET. Comparison of both 9E7.4 conjugates revealed higher nonspecific bone uptakes of 89Zr-DFO-9E7.4 than 64Cu-TE2A-9E7.4. Because of free 89Zr’s tropism for bone when using 89Zr-anti-CD138, 64Cu-anti-CD138 antibody had the most optimal tumor-to-nontarget tissue ratios for translation into humans as a specific new imaging radiopharmaceutical agent in MM.
Adult Stem Cell Functioning in the Tumor Micro-Environment
Authors: Jiang Yuhan , Wells Alan , Sylakowski Kyle , Clark Amanda M. , Ma Bo
Published: May 2019

Tumor progression from an expanded cell population in a primary location to disseminated lethal growths subverts attempts at cures. It has become evident that these steps are driven in a large part by cancer cell-extrinsic signaling from the tumor microenvironment (TME), one cellular component of which is becoming more appreciated for potential modulation of the cancer cells directly and the TME globally. That cell is a heterogenous population referred to as adult mesenchymal stem cells/multipotent stromal cells (MSCs). Herein, we review emerging evidence as to how these cells, both from distant sources, mainly the bone marrow, or local resident cells, can impact the progression of solid tumors. These nascent investigations raise more questions than they answer but paint a picture of an orchestrated web of signals and interactions that can be modulated to impact tumor progression.
Mutations in the Rice OsCHR4 Gene, Encoding a CHD3 Family Chromatin Remodeler, Induce Narrow and Rolled Leaves with Increased Cuticular Wax
Authors: Guo Tingting , Wang Daofeng , Fang Jingjing , Zhao Jinfeng , Yuan Shoujiang , Xiao Langtao , Li Xueyong
Published: May 2019

Leaf blade width, curvature, and cuticular wax are important agronomic traits of rice. Here, we report the rice Oschr4-5 mutant characterized by pleiotropic phenotypes, including narrow and rolled leaves, enhanced cuticular wax deposition and reduced plant height and tiller number. The reduced leaf width is caused by a reduced number of longitudinal veins and increased auxin content. The cuticular wax content was significantly higher in the Oschr4-5 mutant, resulting in reduced water loss rate and enhanced drought tolerance. Molecular characterization reveals that a single-base deletion results in a frame-shift mutation from the second chromodomain of OsCHR4, a CHD3 (chromodomain helicase DNA-binding) family chromatin remodeler, in the Oschr4-5 mutant. Expressions of seven wax biosynthesis genes (GL1-4, WSL4, OsCER7, LACS2, LACS7, ROC4 and BDG) and four auxin biosynthesis genes (YUC2, YUC3, YUC5 and YUC6) was up-regulated in the Oschr4-5 mutant. Chromatin immunoprecipitation assays revealed that the transcriptionally active histone modification H3K4me3 was increased, whereas the repressive H3K27me3 was reduced in the upregulated genes in the Oschr4-5 mutant. Therefore, OsCHR4 regulates leaf morphogenesis and cuticle wax formation by epigenetic modulation of auxin and wax biosynthetic genes expression.
Molecular Docking and Molecular Dynamics (MD) Simulation of Human Anti-Complement Factor H (CFH) Antibody Ab42 and CFH Polypeptide
Authors: Yang Bing , Lin Shu-Jian , Ren Jia-Yi , Liu Tong , Wang Yue-Ming , Li Cheng-Ming , Xu Wen-Wen , He You-Wen , Zheng Wei-Hong , Zhao Jian , Yuan Xiao-Hui , Liao Hua-Xin
Published: May 2019

An understanding of the interaction between the antibody and its targeted antigen and knowing of the epitopes are critical for the development of monoclonal antibody drugs. Complement factor H (CFH) is implied to play a role in tumor growth and metastasis. An autoantibody to CHF is associated with anti-tumor cell activity. The interaction of a human monoclonal antibody Ab42 that was isolated from a cancer patient with CFH polypeptide (pCFH) antigen was analyzed by molecular docking, molecular dynamics (MD) simulation, free energy calculation, and computational alanine scanning (CAS). Experimental alanine scanning (EAS) was then carried out to verify the results of the theoretical calculation. Our results demonstrated that the Ab42 antibody interacts with pCFH by hydrogen bonds through the Tyr315, Ser100, Gly33, and Tyr53 residues on the complementarity-determining regions (CDRs), respectively, with the amino acid residues of Pro441, Ile442, Asp443, Asn444, Ile447, and Thr448 on the pCFH antigen. In conclusion, this study has explored the mechanism of interaction between Ab42 antibody and its targeted antigen by both theoretical and experimental analysis. Our results have important theoretical significance for the design and development of relevant antibody drugs.
Immune System and DNA Repair Defects in Ovarian Cancer: Implications for Locoregional Approaches
Authors: Farolfi Alberto , Gurioli Giorgia , Fugazzola Paola , Burgio Salvatore , Casanova Claudia , Ravaglia Giorgia , Altavilla Amelia , Costantini Matteo , Amadori Andrea , Framarini Massimo , Ansaloni Luca , De Giorgi Ugo
Published: May 2019

In the last few years, substantial progress has been made in the treatment of ovarian cancer, with increased knowledge about the biology of the disease. Ovarian cancer is a neoplasm strongly linked to defects in DNA repair mechanisms, where deficiency in the homologous recombination (HR) system results in a better response of ovarian cancers to therapy, whether platinum-based chemotherapy, anthracyclines, or poly (ADP-ribose) polymerase (PARP) inhibitors. More recently, it has been demonstrated that different ovarian cancer histotypes may have different immunogenicity. Interestingly, defects in HR systems are associated more frequently with higher tumor infiltrating lymphocytes, providing a rationale for developing combination therapy with immune-modulating agents and PARP inhibitors. Again, locoregional therapies combining heat shock and chemotherapy delivery have been shown to induce an anticancer immune response in vitro. Thus, the potential for locoregional therapeutic approaches that may impact the immune system, perhaps in combination with immune-modulating agents or PARP inhibitors, needs to be further explored. With this premise, we reviewed the main biological and clinical data demonstrating a strict interplay between the immune system, DNA repair mechanisms, and intraperitoneal therapies in ovarian cancer, with a focus on potential future therapeutic implications.
Hydroxyproline-Rich Glycoproteins as Markers of Temperature Stress in the Leaves of Brachypodium distachyon
Authors: Pinski Artur , Betekhtin Alexander , Sala Katarzyna , Godel-Jedrychowska Kamila , Kurczynska Ewa , Hasterok Robert
Published: May 2019

Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4 °C) or a high (40 °C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8, JIM13, JIM16, LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11, JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8, JIM13, JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11, JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress.
Experimental Motor Neuron Disease Induced in Mice with Long-Term Repeated Intraperitoneal Injections of Serum from ALS Patients
Authors: Obál Izabella , Nógrádi Bernát , Meszlényi Valéria , Patai Roland , Ricken Gerda , Kovacs Gabor G. , Tripolszki Kornélia , Széll Márta , Siklós László , Engelhardt József I.
Published: May 2019

In an earlier study, signs of commencing degeneration of spinal motor neurons were induced in mice with short-term intraperitoneal injections of immunoglobulin G (IgG) taken from patients with amyotrophic lateral sclerosis (ALS). Since in that study, neither weakness nor loss of motor neurons was noted, to test whether the ALS IgG in this paradigm has the potential to evoke relentless degeneration of motor neurons, treatment with repeated injections over a longer period was carried out. Mice were systematically injected intraperitoneally with serum taken from ALS patients over a 75-day period. At selected time points, the isometric force of the limbs, number of spinal motor neurons and their intracellular calcium levels were determined. Furthermore, markers of glial activation and the motoneuronal uptake of human IgG were monitored. During this period, gliosis and progressive motoneuronal degeneration developed, which led to gradual loss of spinal motor neurons, more than 40% at day 21, along with decreasing muscle strength in the limbs. The inclusion-like accumulation of IgG appeared in the perikarya with the increase of intracellular calcium in the cell bodies and motor nerve terminals. Our results demonstrate that ALS serum can transfer motor neuron disease to mice.
The Relevance of Mass Spectrometry Analysis for Personalized Medicine through Its Successful Application in Cancer “Omics”
Authors: Ciocan-Cartita Cristina Alexandra , Jurj Ancuța , Buse Mihail , Gulei Diana , Braicu Cornelia , Raduly Lajos , Cojocneanu Roxana , Pruteanu Lavinia Lorena , Iuga Cristina Adela , Coza Ovidiu , Berindan-Neagoe Ioana
Published: May 2019

Mass spectrometry (MS) is an essential analytical technology on which the emerging omics domains; such as genomics; transcriptomics; proteomics and metabolomics; are based. This quantifiable technique allows for the identification of thousands of proteins from cell culture; bodily fluids or tissue using either global or targeted strategies; or detection of biologically active metabolites in ultra amounts. The routine performance of MS technology in the oncological field provides a better understanding of human diseases in terms of pathophysiology; prevention; diagnosis and treatment; as well as development of new biomarkers; drugs targets and therapies. In this review; we argue that the recent; successful advances in MS technologies towards cancer omics studies provides a strong rationale for its implementation in biomedicine as a whole.
Remarkable Alteration of PD-L1 Expression after Immune Checkpoint Therapy in Patients with Non-Small-Cell Lung Cancer: Two Autopsy Case Reports
Authors: Takahashi Toshiaki , Tateishi Akiko , Bychkov Andrey , Fukuoka Junya
Published: May 2019

Pembrolizumab is an immune checkpoint inhibitor (ICI), currently recommended as the first-line treatment for patients with advanced non-small-cell lung cancer (NSCLC) showing ≥50% expression of programmed death-ligand 1 (PD-L1). Previously it was reported that platinum-based chemotherapy may change PD-L1 expression in solid cancers. However, no reports addressing alteration of PD-L1 expression after ICI therapy in NSCLC are available so far. The patients were Japanese males 83 and 87 years old, who were diagnosed with NSCLC based on the transbronchial lung biopsies showing sarcomatoid feature with high PD-L1 expression. They received Pembrolizumab, however, passed away with disease progression on day 27 and day 9, respectively. PD-L1, PD1, and CD8 antibodies were applied to pretreatment tumor biopsies and autopsy specimens. Immunoexpression of all the markers was evaluated using Aperio ImageScope. We found that PD-L1 expression decreased significantly from 75.6% to 13.2% and from 100% to 58.8%, in patients 1 and 2, respectively. This alteration was less prominent in the perinecrotic tumor area. A considerable decrease of PD-L1 score was linked with a little effect of Pembrolizumab in our patients. This association might be one of the contributing mechanisms of resistance to ICI and needs further investigation in large-scale studies.
Effects of Sapindus mukorossi Seed Oil on Skin Wound Healing: In Vivo and in Vitro Testing
Authors: Chen Chang-Chih , Nien Chia-Jen , Chen Lih-Geeng , Huang Kuen-Yu , Chang Wei-Jen , Huang Haw-Ming
Published: May 2019

Sapindus mukorossi seed oil is commonly used as a source for biodiesel fuel. Its phytochemical composition is similar to the extracted oil from Sapindus trifoliatus seeds, which exhibit beneficial effects for skin wound healing. Since S. mukorossi seed shows no cyanogenic property, it could be a potential candidate for the treatment of skin wounds. Thus, we evaluated the effectiveness of S. mukorossi seed oil in the treatment of skin wounds. We characterized and quantified the fatty acids and unsaponifiable fractions (including β-sitosterol and δ-tocopherol) contained in S. mukorossi seed-extracted oil by GC-MS and HPLC, respectively. Cell proliferation and migratory ability were evaluated by cell viability and scratch experiments using CCD-966SK cells treated with S. mukorossi oil. The anti-inflammatory effects of the oil were evaluated by measuring the nitric oxide (NO) production in lipopolysaccharide-treated RAW 264.7 cells. Antimicrobial activity tests were performed with Propionibacterium acnes, Staphylococcus aureus, and Candida albicans using a modified Japanese Industrial Standard procedure. Uniform artificial wounds were created on the dorsum of rats. The wounds were treated with a carboxymethyl cellulose (CMC)/hyaluronic acid (HA)/sodium alginate (SA) hydrogel for releasing the S. mukorossi seed oil. The wound sizes were measured photographically for 12 days and were compared to wounds covered with analogous membranes containing a saline solution. Our results showed that the S. mukorossi seed oil used in this study contains abundant monounsaturated fatty acids, β-sitosterol, and δ-tocopherol. In the in vitro tests, S. mukorossi seed oil prompted cell proliferation and migration capability. Additionally, the oil had significant anti-inflammatory and anti-microbial activities. In the in vivo animal experiments, S. mukorossi seed oil-treated wounds revealed acceleration of sequential skin wound healing events after two days of healing. The size of oil-treated wound decreased to half the size of the untreated control after eight days of healing. The results suggest that S. mukorossi seed oil could be a potential source for promoting skin wound healing.
Knockdown of NANOG Reduces Cell Proliferation and Induces G0/G1 Cell Cycle Arrest in Human Adipose Stem Cells
Authors: Pitrone Maria , Pizzolanti Giuseppe , Coppola Antonina , Tomasello Laura , Martorana Stefania , Pantuso Gianni , Giordano Carla
Published: May 2019

The core components of regenerative medicine are stem cells with high self-renewal and tissue regeneration potentials. Adult stem cells can be obtained from many organs and tissues. NANOG, SOX2 and OCT4 represent the core regulatory network that suppresses differentiation-associated genes, maintaining the pluripotency of mesenchymal stem cells. The roles of NANOG in maintaining self-renewal and undifferentiated status of adult stem cells are still not perfectly established. In this study we define the effects of downregulation of NANOG in maintaining self-renewal and undifferentiated state in mesenchymal stem cells (MSCs) derived from subcutaneous adipose tissue (hASCs). hASCs were expanded and transfected in vitro with short hairpin Lentivirus targeting NANOG. Gene suppressions were achieved at both transcript and proteome levels. The effect of NANOG knockdown on proliferation after 10 passages and on the cell cycle was evaluated by proliferation assay, colony forming unit (CFU), qRT-PCR and cell cycle analysis by flow-cytometry. Moreover, NANOG involvement in differentiation ability was evaluated. We report that downregulation of NANOG revealed a decrease in the proliferation and differentiation rate, inducing cell cycle arrest by increasing p27/CDKN1B (Cyclin-dependent kinase inhibitor 1B) and p21/CDKN1A (Cyclin-dependent kinase inhibitor 1A) through p53 and regulate DLK1/PREF1. Furthermore, NANOG induced downregulation of DNMT1, a major DNA methyltransferase responsible for maintaining methylation status during DNA replication probably involved in cell cycle regulation. Our study confirms that NANOG regulates the complex transcription network of plasticity of the cells, inducing cell cycle arrest and reducing differentiation potential.
Impact of Periprosthetic Fibroblast-Like Cells on Osteoclastogenesis in Co-Culture with Peripheral Blood Mononuclear Cells Varies Depending on Culture System
Authors: Koehler Miriam I. , Hartmann Eliza S. , Schluessel Sabine , Beck Felicitas , Redeker Julia I. , Schmitt Baerbel , Unger Marina , van Griensven Martijn , Summer Burkhard , Fottner Andreas , Mayer-Wagner Susanne
Published: May 2019

Co-culture studies investigating the role of periprosthetic fibroblasts (PPFs) in inflammatory osteoclastogenesis reveal contrary results, partly showing an osteoprotective function of fibroblasts and high OPG expression in monolayer. These data disagree with molecular analyses of original periosteolytic tissues. In order to find a more reliable model, PPFs were co-cultivated with peripheral blood mononuclear cells (PBMCs) in a transwell system and compared to conventional monolayer cultures. The gene expression of key regulators of osteoclastogenesis (macrophage colony-stimulating factor (MCSF), receptor activator of NF-κB ligand (RANK-L), osteoprotegerin (OPG), and tumor necrosis factor alpha (TNFα)) as well as the ability of bone resorption were analyzed. In monolayer co-cultures, PPFs executed an osteoprotective function with high OPG-expression, low RANK-L/OPG ratios, and a resulting inhibition of osteolysis even in the presence of MCSF and RANK-L. For transwell co-cultures, profound changes in gene expression, with a more than hundredfold decrease of OPG and a significant upregulation of TNFα were observed. In conclusion, we were able to show that a change of culture conditions towards a transwell system resulted in a considerably more osteoclastogenic gene expression profile, being closer to findings in original periosteolytic tissues. This study therefore presents an interesting approach for a more reliable in vitro model to examine the role of fibroblasts in periprosthetic osteoclastogenesis in the future.
Salt Tolerance Improvement in Rice through Efficient SNP Marker-Assisted Selection Coupled with Speed-Breeding
Authors: Rana Md Masud , Takamatsu Takeshi , Baslam Marouane , Kaneko Kentaro , Itoh Kimiko , Harada Naoki , Sugiyama Toshie , Ohnishi Takayuki , Kinoshita Tetsu , Takagi Hiroki , Mitsui Toshiaki
Published: May 2019

Salinity critically limits rice metabolism, growth, and productivity worldwide. Improvement of the salt resistance of locally grown high-yielding cultivars is a slow process. The objective of this study was to develop a new salt-tolerant rice germplasm using speed-breeding. Here, we precisely introgressed the hst1 gene, transferring salinity tolerance from “Kaijin” into high-yielding “Yukinko-mai” (WT) rice through single nucleotide polymorphism (SNP) marker-assisted selection. Using a biotron speed-breeding technique, we developed a BC3F3 population, named “YNU31-2-4”, in six generations and 17 months. High-resolution genotyping by whole-genome sequencing revealed that the BC3F2 genome had 93.5% similarity to the WT and fixed only 2.7% of donor parent alleles. Functional annotation of BC3F2 variants along with field assessment data indicated that “YNU31-2-4” plants carrying the hst1 gene had similar agronomic traits to the WT under normal growth condition. “YNU31-2-4” seedlings subjected to salt stress (125 mM NaCl) had a significantly higher survival rate and increased shoot and root biomasses than the WT. At the tissue level, quantitative and electron probe microanalyzer studies indicated that “YNU31-2-4” seedlings avoided Na+ accumulation in shoots under salt stress. The “YNU31-2-4” plants showed an improved phenotype with significantly higher net CO2 assimilation and lower yield decline than WT under salt stress at the reproductive stage. “YNU31-2-4” is a potential candidate for a new rice cultivar that is highly tolerant to salt stress at the seedling and reproductive stages, and which might maintain yields under a changing global climate.
The Biology of the Nuclear Envelope and Its Implications in Cancer Biology
Authors: Alvarado-Kristensson Maria , Rosselló Catalina Ana
Published: May 2019

The formation of the nuclear envelope and the subsequent compartmentalization of the genome is a defining feature of eukaryotes. Traditionally, the nuclear envelope was purely viewed as a physical barrier to preserve genetic material in eukaryotic cells. However, in the last few decades, it has been revealed to be a critical cellular component in controlling gene expression and has been implicated in several human diseases. In cancer, the relevance of the cell nucleus was first reported in the mid-1800s when an altered nuclear morphology was observed in tumor cells. This review aims to give a current and comprehensive view of the role of the nuclear envelope on cancer first by recapitulating the changes of the nuclear envelope during cell division, second, by reviewing the role of the nuclear envelope in cell cycle regulation, signaling, and the regulation of the genome, and finally, by addressing the nuclear envelope link to cell migration and metastasis and its use in cancer prognosis.
Prostate Cancer and Bone Metastases: The Underlying Mechanisms
Authors: Wong Sok Kuan , Mohamad Nur-Vaizura , Giaze Tijjani Rabiu , Chin Kok-Yong , Mohamed Norazlina , Ima-Nirwana Soelaiman
Published: May 2019

Patients with advanced prostate cancer often develop bone metastases, leading to bone pain, skeletal fracture, and increased mortality. Bone provides a hospitable microenvironment to tumor cells. The disease manifestation is driven by the interaction between invading tumor cells, bone-forming osteoblasts, and bone-resorbing osteoclasts. The increased level of osteoclast-activating factor (parathyroid hormone-related peptide, PTHrP) is believed to induce bone resorption by upregulating receptor activator of nuclear factor-kappa B ligand (RANKL) and the release of various growth factors into the bone microenvironment to enhance cancer cell growth. However, the underlying molecular mechanisms remain poorly understood. This review outlines the possible molecular mechanisms involved in governing bone metastases driven by prostate cancer, which further provide the basis in searching for new molecular targets for the development of potential therapy.
Crucial Role of Extracellular Vesicles in Bronchial Asthma
Authors: Nagano Tatsuya , Katsurada Masahiro , Dokuni Ryota , Hazama Daisuke , Kiriu Tatsunori , Umezawa Kanoko , Kobayashi Kazuyuki , Nishimura Yoshihiro
Published: May 2019

Extracellular vesicles (EVs) are circulating vesicles secreted by various cell types. EVs are classified into three groups according to size, structural components, and generation process of vesicles: exosomes, microvesicles, and apoptotic bodies. Recently, EVs have been considered to be crucial for cell-to-cell communications and homeostasis because they contain intracellular proteins and nucleic acids. Epithelial cells from mice suffering from bronchial asthma (BA) secrete more EVs and suppress inflammation-induced EV production. Moreover, microarray analyses of bronchoalveolar lavage fluid have revealed that several microRNAs are useful novel biomarkers of BA. Mesenchymal stromal cell-derived EVs are possible candidates of novel BA therapy. In this review, we highlight the biologic roles of EVs in BA and review novel EV-targeted therapy to help understanding by clinicians and biologists.