bluuebunny/crossref_metadata_2025 huggingface dataset:
Heat and Flow Control in Cavity with Cold Circular Cylinder Placed in Non-Newtonian Fluid by Performing Finite Element Simulations
Authors: Bilal Sardar , Khan Noor Zeb , Shah Imtiaz Ali , Awrejcewicz Jan , Akgül Ali , Riaz Muhammad Bilal
Published: December 2021

A study on strategies regarding advancement in heat transfer characteristics in two-dimensional closed domains by placing cold cylinders is conducted. This effort is undertaken due to the fact that active and passive control in heat transmission is connected with provision of temperature differences at different locations of enclosures. Based on the experiments, researchers have concluded that placement of cold cylinder in non-uniformly distributed heat in a cavity is the most effective technique to enrich heat transfer rate, along with reducing the the waste of extra heat generation in processes such as polymer and aero dynamical extrusion, glass cooling, refrigeration, heating and cooling systems. Thus, the prime goal of this work is to outline heat and flow characteristics of non-linear fluid occupied in a square enclosure with adjustment of the cold cylinder. Heat transfer attributes are incorporated by accounting buoyancy forces and forming coupling of molecular diffusion of fluid within the flow domain. Formulation of the problem in dimensionless form is attained by encapsulating the aspects of natural convection in view of principal partial differential equations. Parametric study for governing expressions is computed numerically with the finite element method based on COMSOL Multiphysics version 5.6. Quadric interpolating functions are used to obtain information about velocity and temperature on nodes in elements. Hybrid meshing is manifested for discretization of the domain into rectangular and triangular elements. For the optimized variation in flow structures, prospective parameters are varied from 0.5≤n≤1.5, 5 ≤ Pr ≤ 35 and 102 ≤ Ra ≤106. The achieved results are projected graphically through streamlines, isotherms, and local and average Nusselt numbers. Tabular data for kinetic energy and wall heat flux are also calculated. It is inferred through the analysis that, with uplift in the Rayleigh number Ra elevation in the magnitude of kinetic energy and convective heat transfer arises, whereas the reverse pattern is depicted versus the power–law index n.
Photocatalytic Degradation of Methylene Blue and Metanil Yellow Dyes Using Green Synthesized Zinc Oxide (ZnO) Nanocrystals
Authors: Priyadharshini S. Shwetha , Shubha Jayachamarajapura Pranesh , Shivalingappa Jaydev , Adil Syed Farooq , Kuniyil Mufsir , Hatshan Mohammad Rafe , Shaik Baji , Kavalli Kiran
Published: December 2021

In this work, ZnO nanocrystals (NCs) have been effectively synthesized by a simple, efficient and cost-effective method using coconut husk extract as a novel fuel. The synthesized NCs are characterized by UV-Vis, XRD, FT-IR, SEM, EDX, Raman and PL studies. The obtained ZnO were found to be UV-active with a bandgap of 2.93 eV. The X-ray diffraction pattern confirms the crystallinity of the ZnO with hexagonally structured ZnO with a crystallite size of 48 nm, while the SEM analysis reveals the hexagonal bipyramid morphology. Photocatalytic activities of the synthesized ZnO NCs are used to degrade methylene blue and metanil yellow dyes.
Effect of Aluminum on Microstructure and Mechanical Properties of Weld Metal of Q960 Steel
Authors: Zou Zongxuan , Liu Zhengjun , Ai Xingyu , Wu Dan
Published: December 2021

High-strength low-alloy (HSLA) steel is used in important steel structural members because of its strength and plastic toughness. Q960 steel is HSLA steel obtained by adding an appropriate amount of alloy elements and quenching and tempering treatment on the basis of ordinary low-carbon steel. This kind of steel has strong hardenability due to the alloy elements added. Cold cracks, embrittlement and softening of the heat-affected zone easily occur after welding. In particular, the low-temperature impact toughness cannot meet the requirements and limits its use. In this paper, self-shielded welding is used to adjust the content of aluminum in flux-cored wire. The relationship between weld metal (WM) microstructure and strength and properties was studied by tensile test and impact test, and the influence mechanism of Al content on weld metal microstructure and properties was analyzed. The results show that when the content of Al is 0.21%, the impact energy at 0 °C~−60 °C is the best, the tensile strength can reach 1035 MPA and the number of pores is small. The size of inclusions in WM is mostly less than 1.0 μm Al2O3 spherical oxide. It can become the center of acicular ferrite (AF) and increase the nucleation probability. However, with the increase of Al content, large irregular AlN inclusions are produced, which reduces the tensile strength and impact energy of the welded joint.
HMGA1 Has Predictive Value in Response to Chemotherapy in Gastric Cancer
Authors: Pádua Diana , Pinto Débora Filipa , Figueira Paula , Pereira Carlos Filipe , Almeida Raquel , Mesquita Patrícia
Published: December 2021

Gastric cancer is a serious health problem worldwide. Although its incidence is decreasing, the five-year survival rate remains low. Thus, it is essential to identify new biomarkers that could promote better diagnosis and treatment of patients with gastric cancer. High-mobility group AT-hook 1 (HMGA1) is a non-histone, chromatin-binding protein that has been found overexpressed in several tumor types. It has been correlated with invasion, metastasis, and drug resistance, leading to worse patient survival. The aim of this work was to evaluate the clinical value of HMGA1 in gastric cancer. HMGA1 expression was analyzed by immunohistochemistry in a single hospital series (n = 323) of gastric adenocarcinoma cases (stages I to IV) with clinicopathological and treatment data. In this series, HMGA1 expression showed no significant relevance as a prognostic biomarker. Nevertheless, a significantly better overall survival was observed in cases with high levels of HMGA1 when they were treated with chemotherapy, compared to the nontreated ones, implying that they can benefit more from treatment than patients with low expression of HMGA1. We thereby show for the first time that HMGA1 expression has a substantial value as a biomarker of response to chemotherapy in gastric cancer.
Quantitative Analysis of SARS-CoV-2 Antibody Status between Patients with Cancer and Healthy Individuals with Extended Vaccination Dosing Intervals in Canada
Authors: Robinson Andrew , Mazurek Andrew , Xu Minqi , Gong Yanping
Published: December 2021

(1) Background: To date, data addressing the antibody response of cancer patients to SARS-CoV-2 vaccines are limited. To our knowledge, this is the first report to evaluate humoral immunity. responses in Canadian cancer patients. (2) Methods: 116 cancer patients and 35 healthy participants were enrolled in this cross-sectional study. The interval between the first and second doses were closely matched during analysis. IgG antibodies against the SARS-CoV-2 spike receptor–binding domain were determined using an enzyme-linked immunosorbent assay (ELISA). (3) Results: Following two doses of SARS-CoV-2 vaccine (including BNT162b2, AZD1222, and mRNA-1273), the mean serum anti-spike protein antibody level was 382.4 BAU/mL (binding antibody unit, SD ± 9.4) in the control group, 265.8 BAU/mL (±145.7) in solid cancer patients, and 168.2 BAU/mL (±172.9) in hematological cancer patients. Observed differences were significantly lower in both solid and hematological groups when comparing to the control group (p ≤ 0.0001). In solid cancer group, patients with cytotoxic chemotherapy demonstrated significantly lower antibody levels (p < 0.01), whereas the rest of the patients showed similar antibody levels as the healthy control. Antibody levels were lower in those on treatment than those off treatment in patients with hematological malignancies (p < 0.0001) but not for those with solid cancers (p = 0.4553). (4) Conclusions: After two doses of the SARS-CoV-2 vaccination, patients with solid and hematological malignancies demonstrated impaired serological responses. This was particularly prominent if there was cytotoxic chemotherapy or systemic therapy in solid and hematological cancer, respectively.
Head and Neck Paragangliomas in the Czech Republic: Management at the Otorhinolaryngology Department
Authors: Guha Anasuya , Chovanec Martin
Published: December 2021

Head and neck paragangliomas (HNPGLs) are rare neuroendocrine tumors, comprising only 3% of all head and neck tumors. Early diagnosis forms an integral part of the management of these tumors. The two main aims of any treatment approach are long-term tumor control and minimal cranial nerve morbidity. The scope of this article is to present our case series of HNPGLs to stress most important clinical aspects of their presentation as well as critical issues of their complex management. Thirty patients with suspected HNPGLs were referred to our otorhinolaryngology clinic for surgical consultation between 2016–2020. We assessed the demographical pattern, clinicoradiological correlation, as well as type and outcome of treatment. A total of 42 non-secretory tumors were diagnosed—16.7% were incidental findings and 97% patients had benign tumors. Six patients had multiple tumors. Jugular paragangliomas were the most commonly treated tumors. Tumor control was achieved in nearly 96% of operated patients with minimal cranial nerve morbidity. Surgery is curative in most cases and should be considered as frontline treatment modality in experienced hands for younger patients, hereditary and secretory tumors. Cranial nerve dysfunction associated with tumor encasement is a negative prognostic factor for both surgery and radiotherapy. Multifocal tumors and metastasis are difficult to treat, even with early detection using genetic analysis. Detecting malignancy in HNPGLs is challenging due to the lack of histomorphological criteria; therefore, limited lymph node dissection should be considered, even in the absence of clinical and radiological signs of metastasis in carotid body, vagal, and jugular paragangliomas.
The Listsize Capacity of the Gaussian Channel with Decoder Assistance
Authors: Lapidoth Amos , Yan Yiming
Published: December 2021

The listsize capacity is computed for the Gaussian channel with a helper that—cognizant of the channel-noise sequence but not of the transmitted message—provides the decoder with a rate-limited description of said sequence. This capacity is shown to equal the sum of the cutoff rate of the Gaussian channel without help and the rate of help. In particular, zero-rate help raises the listsize capacity from zero to the cutoff rate. This is achieved by having the helper provide the decoder with a sufficiently fine quantization of the normalized squared Euclidean norm of the noise sequence.
Common Environmental Effects on Quantum Thermal Transistor
Authors: Liu Yu-Qiang , Yu Deng-Hui , Yu Chang-Shui
Published: December 2021

Quantum thermal transistor is a microscopic thermodynamical device that can modulate and amplify heat current through two terminals by the weak heat current at the third terminal. Here we study the common environmental effects on a quantum thermal transistor made up of three strong-coupling qubits. It is shown that the functions of the thermal transistor can be maintained and the amplification rate can be modestly enhanced by the skillfully designed common environments. In particular, the presence of a dark state in the case of the completely correlated transitions can provide an additional external channel to control the heat currents without any disturbance of the amplification rate. These results show that common environmental effects can offer new insights into improving the performance of quantum thermal devices.
The Role of Public Spending in CO2 Emissions Reduction in Polish Regions: An LMDI Decomposition Approach
Authors: Wójtowicz Katarzyna Aleksandra , Szołno-Koguc Jolanta Małgorzata , Braun Jan
Published: December 2021

Global climate change and air pollution are critical environmental problems in the modern world. Therefore, the reduction of CO2 emissions has long been a crucial challenge for individual countries. In this area, numerous technological, legal, and economic solutions are used. The purpose of this article is to examine the impact of public spending on the level of CO2 emissions in Polish regions. The study uses the logarithmic mean Divisia index (LMDI) method, proposing a unique proprietary set of factors that influence the level of CO2 emissions. The results of the study confirm that public expenditure contributes to reducing CO2 emissions at the regional level, while environmental expenditure is counterproductive. This tendency is observed mainly in regions with a high level of socioeconomic development, higher energy consumption, and high carbon emissions. The failure of environmental spending to reduce carbon dioxide emissions in Polish regions is explained by the “green paradox” hypothesis.
An Evaluation of the Performance of a Ground-to-Air Heat Exchanger in Different Ventilation Scenarios in a Single-Family Home in a Climate Characterized by Cold Winters and Hot Summers
Authors: Skotnicka-Siepsiak Aldona
Published: December 2021

In the present study, the real-world performance of a ground-to-air heat exchanger (GAHE) was analyzed in the Polish climate which is characterized by warm summers and cold winters. The heat exchanger’s performance was monitored over a period of three years (2017 to 2019), and real-world conditions were compared with a Typical Meteorological Year (TMY). The aim of the study was to assess the exchanger’s energy-efficiency potential in various ventilation scenarios in a single-family home under variable real-world conditions, rather than to simply determine its heating and cooling capacity. The analyzed single-family home was a modern, single-story building with a usable floor area of 115 m2. The building’s thermal insulation and airtightness met stringent energy-efficiency standards. Energy consumption in a building equipped with a natural ventilation system was compared with three other scenarios: ventilation coupled with a GAHE, mechanical ventilation with heat recovery and a high-efficiency heat exchanger (HE), and mechanical ventilation with heat recovery coupled with a GAHE. Sensible heating and cooling loads were calculated based on standard ISO 13790:2008, and latent heating and cooling loads were also included in the energy balance. During the year, the GAHE generated around 257.6 W of heating energy per hour and 124.7 W of cooling energy per hour. Presented results can be used to select the optimal HVAC system scenarios for engineering projects as well as private investors.
Smart Cities and Urban Management—A Section of Energies (ISSN 1996-1073)
Authors: Berardi Umberto
Published: December 2021

The aim of Energies’ Smart Cities and Urban Management Section is to present new research results and new proven practices aimed at optimizing the energy use of smart cities and improving the energy efficiency in urban management [...]
Factors Influencing the Renewable Energy Consumption in Selected European Countries
Authors: Polcyn Jan , Us Yana , Lyulyov Oleksii , Pimonenko Tetyana , Kwilinski Aleksy
Published: December 2021

The overcoming of the issues on energy crisis and inequality have become the priorities as far developing as developed countries are concerned. Moreover, energy inequality has increased due to the shortage of natural gas and rising energy prices in retaliation to the economic recovery affected by the COVID-19 pandemic. This study aims to verify the linkage between the growth of renewable energy consumption and the country’s economic advancement. In this context, this paper determines the main driving forces of renewable energy consumption in European countries during 2000–2018. The annual data for panel regression analysis are retrieved from the OECD. Stat and World Bank Open Data. This empirical analysis employed a set of estimation procedures such as the panel unit root test (Levin, Lin & Chu; Im, Pesaran, Shin W-Stat; ADF-Fisher Chi-square; and PP-Fisher Chi-square methods), the Pearson correlation, fixed- and random-effects models, generalized method of moments (GMM), Hausman and the robustness tests. The results from the Hausman test ratified that the fixed-effects regression model is more suitable for involved panel balanced data. The results of fixed-effects regression and GMM identified the statistically significant and positive relationship between the share of renewable energy consumption of total final energy consumption, GDP per capita, and CO2 emissions per capita for the overall sample. In turn, the total labor force, the gross capital formation, and production-based CO2 intensity are inversely related to renewable energy consumption. The identified effects could provide some insights for policymakers to improve the renewable energy sector towards gaining sustainable economic development.
A Method for the Segregation of Emulsion Inner Phase Droplets Using Imbibition Process in Porous Material
Authors: Błaszczyk Mariola M. , Przybysz Łukasz
Published: December 2021

The process of forming an emulsion is an energy-consuming process. The smaller the internal phase droplets we want to produce and the closer the droplets are in size to each other (monodisperse), the more energy we need to put into the system. Generating energy carries a high economic cost, as well as a high environmental footprint. Considering the fact that dispersive systems are widely used in various fields of life, it is necessary to search for other, less-energy-intensive methods that will allow the creation of dispersive systems with adequate performance and minimal energy input. Therefore, an alternative way to obtain emulsions characterized by small droplet sizes was proposed by using an imbibition process in porous materials. By applying this technique, it was possible to obtain average droplet sizes at least half the size of the base emulsion while reducing the polydispersity by about 40%. Oil-in-water emulsions in which vegetable oil or kerosene is the oily phase were tested. The studies were carried out at three different volume concentrations of the emulsions. Detailed analyses of diameter distributions and emulsion concentrations are presented. In addition, the advantages and limitations of the method are presented and the potential for its application is indicated.
Vector-Field Visualization of the Total Reflection of the EM Wave by an SRR Structure at the Magnetic Resonance
Authors: Budnarowska Magdalena , Rafalski Szymon , Mizeraczyk Jerzy
Published: December 2021

Metamaterials are artificially structured composite media with a unique electromagnetic (EM) response that is absent from naturally occurring materials, which appears counterintuitive and aggravates traditional difficulties in perceiving the behavior of EM waves. The aim of this study was to better understand the interaction of EM waves with metamaterials by virtual visualizing the accompanying physical phenomena. Over the years, virtual visualization of EM wave interactions with metamaterials has proven to be a powerful tool for explaining many phenomena that occur in metamaterials. In this study, we performed virtual visualization of the interaction of an EM plane wave with a split-ring resonator (SRR) metamaterial structure, employing CST Studio software for modeling and comprehensive simulations of high-frequency EM fields of 3D objects. The SRR structure was designed to have its magnetic resonance at the frequency f = 23.69 GHz, which is of interest for antennas supporting wireless microwave point-to-point communication systems (e.g., in satellite systems). Our numerical calculations of the coefficients of absorption, reflection, and transmission of the EM plane wave incident on the SRR structure showed that the SRR structure totally reflected the plane EM wave at the magnetic resonance frequency. Therefore, we focused our research on checking whether the results of numerical calculations could be confirmed by visualizing the total reflection phenomenon on the SRR structure. The performed vector-field visualization resulted in 2D vector maps of the electric and magnetic fields around the SRR structure during the wave period, which demonstrated the existence of characteristic features of the total reflection phenomenon when the EM plane interacted with the studied SRR, i.e., no EM field behind the SRR structure and the standing electric and magnetic waves before the SRR structure, thus, confirming the numerical calculations visually. For deeper understanding the interaction of the EM plane wave with the SRR structure of reflection characteristics at the magnetic resonance frequency f = 23.69 GH, we also visualized the SRR structure response at the frequency f = 21 GHz, i.e., at the so-called detuned frequency. As expected, at the detuned frequency, the SRR structure lost its metamaterial properties and the obtained 2D vector maps of the electric and magnetic fields around the SRR structure during the wave period showed the transmitted EM wave behind the SRR structure and no EM (fully) standing waves before the SRR structure. The visualizations presented in this study are both unique educational presentations to help understand the interaction of EM plane waves with the SRR structure of reflection characteristics at the magnetic resonance and detuned frequencies.
Calculation of Building Heat Losses through Slab-on-Ground Structures Based on Soil Temperature Measured In Situ
Authors: Pokorska-Silva Iwona , Kadela Marta , Orlik-Kożdoń Bożena , Fedorowicz Lidia
Published: December 2021

The article aims to assess the effects of soil temperature measured in situ on the heat loss analyses of a building. Numerical analyses and in situ measurements of soil temperature profiles for real conditions under a residential building (profile I) in Poland and under the area outside the building (profile II) were performed. Based on the measurement results, a proprietary geometric model of the partition was proposed. The heat flux and heat flow results obtained for reliable models are 4.9% and 6.9% higher compared to a model based on a typical meteorological year for the wall–foundation system and 10.0% and 10.1% higher for the slab-on-ground structure for profile I. The adoption of temperatures from the area outside the building as the boundary condition (profile II) results in greater differences between the obtained results. The difference in heat flow obtained in the numerical analyses for profiles I and II is about 2 W/m2, both for the wall–foundation system and for the slab-on-ground structure calculations. The adoption of temperatures for the ground outside the building led to overestimation in the heat flux calculations, this being due to lower temperatures in these particular layers of the ground.
Optimization of Train Energy Cooperation Using Scheduled Service Time Reserve
Authors: Urbaniak Michał , Kardas-Cinal Ewa
Published: December 2021

The main aim of the paper was to develop an innovative approach to the preliminary estimation possibility of train energy cooperation based on data from timetables, without traction calculations. The article points out the need to strive for sustainable and environmentally friendly transport. It was pointed out that rail transport using electric traction is one of the more ecological branches of transport. It also offers a number of possibilities for improving energy efficiency and reducing energy consumption, one of which is the recovery and reuse of energy lost in the electrodynamic braking process. The main ways in which such energy can be used are listed, but the focus is mainly on the aspect of the so-called “energy cooperation” of trains. Then, the current state of the research and knowledge on the narrowed scope of rail traffic management and recuperative braking in rail transportation is presented; an innovative approach to the indirect optimization of the amount of recuperated electrical energy immediately usable by another vehicle by maximizing the duration of energy co-optimization is proposed; and the authors’ function is proposed to enable optimization, taking into account various parameters. The optimization was performed with the use of a proprietary, multi-criteria objective function and the Solver optimization module. Finally, the results obtained using the proposed approach are presented and illustrated with the example of train timetables used by some of the largest railway stations in Poland.
Capture of Pollutants from Exhaust Gases by Low-Temperature Heating Surfaces
Authors: Yang Zongming , Kornienko Victoria , Radchenko Mykola , Radchenko Andrii , Radchenko Roman , Pavlenko Anatoliy
Published: December 2021

One of the most effective methods towards improving the environmental safety of combustion engines is the application of specially prepared water-fuel emulsions (WFE). The application of WFE makes it possible to reduce primary sulfur fuel consumption and reveals the possibility of capturing the pollutants from exhaust gases by applying condensing low-temperature heating surfaces (LTHS). In order to realize such a double effect, it is necessary to investigate the pollution processes on condensing LTHS of exhaust gas boilers (EGB), especially the process of low-temperature condensing a sulfuric acid vapor from exhaust gases to investigate the influence of condensing LTHS on the intensity of pollutants captured from the exhaust gases. The aim of this research is to assess the influence of the intensity of pollutants captured from exhaust gases by condensing LTHS in dependence of water content in WFE combustion. Investigations were carried out at a special experimental setup. The processing of the results of the experimental studies was carried out using the computer universal statistical graphic system Statgraphics. Results have shown that in the presence of a condensing heating surface, the degree of capture (purification) of pollutants from the exhaust gas flow is up to 0.5–0.6.
HAUSP Is a Key Epigenetic Regulator of the Chromatin Effector Proteins
Authors: Abdullah Omeima , Alhosin Mahmoud
Published: December 2021

HAUSP (herpes virus-associated ubiquitin-specific protease), also known as Ubiquitin Specific Protease 7, plays critical roles in cellular processes, such as chromatin biology and epigenetics, through the regulation of different signaling pathways. HAUSP is a main partner of the “Epigenetic Code Replication Machinery,” ECREM, a large protein complex that includes several epigenetic players, such as the ubiquitin-like containing plant homeodomain (PHD) and an interesting new gene (RING), finger domains 1 (UHRF1), as well as DNA methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), histone methyltransferase G9a, and histone acetyltransferase TIP60. Due to its deubiquitinase activity and its ability to team up through direct interactions with several epigenetic regulators, mainly UHRF1, DNMT1, TIP60, the histone lysine methyltransferase EZH2, and the lysine-specific histone demethylase LSD1, HAUSP positions itself at the top of the regulatory hierarchies involved in epigenetic silencing of tumor suppressor genes in cancer. This review highlights the increasing role of HAUSP as an epigenetic master regulator that governs a set of epigenetic players involved in both the maintenance of DNA methylation and histone post-translational modifications.
A Nonsense Variant in CCDC65 Gene Causes Respiratory Failure Associated with Increased Lamb Mortality in French Lacaune Dairy Sheep
Authors: Ben Braiek Maxime , Moreno-Romieux Carole , Allain Charlotte , Bardou Philippe , Bordes Arnaud , Debat Frédéric , Drögemüller Cord , Plisson-Petit Florence , Portes David , Sarry Julien , Tadi Némuel , Woloszyn Florent , Fabre Stéphane
Published: December 2021

We recently demonstrated that the Lacaune deficient homozygous haplotype 6 (LDHH6) potentially hosts a recessive perinatal lethal mutation in Lacaune dairy sheep mapped on OAR3. In the present study, we have analyzed the whole-genome sequences of two Lacaune ram heterozygous carriers of LDHH6. After variant calling and filtering against the variants of 86 non-carrier rams, we have identified a single nucleotide variant (SNV) in the two LDHH6 carriers whose variant allele induced a premature stop codon (p.Glu111*) in the Coiled-Coil Domain Containing 65 (CCDC65) gene. CCDC65 is involved in the assembly of the nexin-dynein regulatory complex for the formation of microtubules in ciliated cells. In order to identify the phenotype in homozygous sheep, we generated at-risk matings (n = 17) between rams and ewes heterozygous for the candidate variant in CCDC65. A total of 16 lambs were born alive with five genotyped as homozygous carriers. The homozygous lambs suffered from respiratory problems, and four of them died within the first month of life. At necropsy, we observed a broad hepatization of lung lobes possibly induced by infectious pneumonia. The management of this lethal recessive allele (frequency of 0.06) through reasoned mating in the Lacaune sheep selection schemes could reduce lamb mortality by 2%.
Heterozygous De Novo Truncating Mutation of Nucleolin in an ASD Individual Disrupts Its Nucleolar Localization
Authors: Sheikh Taimoor I. , Harripaul Ricardo , Vasli Nasim , Ghadami Majid , Santangelo Susan L. , Ayub Muhammad , Sasanfar Roksana , Vincent John B.
Published: December 2021

Nucleolin (NCL/C23; OMIM: 164035) is a major nucleolar protein that plays a critical role in multiple processes, including ribosome assembly and maturation, chromatin decondensation, and pre-rRNA transcription. Due to its diverse functions, nucleolin has frequently been implicated in pathological processes, including cancer and viral infection. We recently identified a de novo frameshifting indel mutation of NCL, p.Gly664Glufs*70, through whole-exome sequencing of autism spectrum disorder trios. Through the transfection of constructs encoding either a wild-type human nucleolin or a mutant nucleolin with the same C-terminal sequence predicted for the autism proband, and by using co-localization with the nucleophosmin (NPM; B23) protein, we have shown that the nucleolin mutation leads to mislocalization of the NCL protein from the nucleolus to the nucleoplasm. Moreover, a construct with a nonsense mutation at the same residue, p.Gly664*, shows a very similar effect on the location of the NCL protein, thus confirming the presence of a predicted nucleolar location signal in this region of the NCL protein. Real-time fluorescence recovery experiments show significant changes in the kinetics and mobility of mutant NCL protein in the nucleoplasm of HEK293Tcells. Several other studies also report de novoNCL mutations in ASD or neurodevelopmental disorders. The altered mislocalization and dynamics of mutant NCL (p.G664Glufs*70/p.G664*) may have relevance to the etiopathlogy of NCL-related ASD and other neurodevelopmental phenotypes.
International Overview of Somatic Dysfunction Assessment and Treatment in Osteopathic Research: A Scoping Review
Authors: Tramontano Marco , Tamburella Federica , Dal Farra Fulvio , Bergna Andrea , Lunghi Christian , Innocenti Mattia , Cavera Fabio , Savini Federica , Manzo Vincenzo , D’Alessandro Giandomenico
Published: December 2021

Background: Osteopathic manipulative treatment (OMT) is a patient-centred, whole-body intervention aimed at enhance the person’s self-regulation. OMT interventions are focused on somatic dysfunctions (SD) that can be defined as an altered regulative function associated with inflammatory signs palpable in the body framework in different body regions. The conceptual model that sustains SD, as well as its usefulness for the osteopathic profession, is still being discussed by the osteopathic community. Understanding the role and the application of SD is the aim of this scoping review. Methods: A literature search was carried out through the main biomedical databases: Pubmed (Medline), Cochrane, Central (Cochrane), Embase, PEDro and Scopus. Grey literature was considered via Google Scholar and the Osteopathic Research Web. The review was prepared by referring to the “Preferred Reporting Items for Systematic reviews and Meta-Analysis extension for Scoping Reviews” (PRISMA-ScR). Results: A total of 37,279 records were identified through database searching and other sources. After the duplicates were removed, 27,023 titles and abstracts were screened. A total of 1495 full-text articles were assessed for eligibility. The qualitative synthesis included 280 studies. Conclusions: Treating SD is an important part of osteopathic practice that varies from country to country. SD should be considered as a clinical value that assists in the clinical assessment and guides the decision-making process of osteopathic practitioners. Further studies should be designed to better understand why and how to choose the different assessment and intervention modalities to approach SD and to evaluate new osteopathic models.
A Smart Healthcare Knowledge Service Framework for Hierarchical Medical Treatment System
Authors: Xie Yi , Gu Dongxiao , Wang Xiaoyu , Yang Xuejie , Zhao Wang , Khakimova Aida K. , Liu Hu
Published: December 2021

This paper reveals the research hotspots and development directions of case-based reasoning in the field of health care, and proposes the framework and key technologies of medical knowledge service systems based on case-based reasoning (CBR) in the big data environment. The 2124 articles on medical CBR in the Web of Science were visualized and analyzed using a bibliometrics method, and a CBR-based knowledge service system framework was constructed in the medical Internet of all people, things and data resources environment. An intelligent construction method for the clinical medical case base and the gray case knowledge reasoning model were proposed. A cloud-edge collaboration knowledge service system was developed and applied in a pilot project. Compared with other diagnostic tools, the system provides case-based explanations for its predicted results, making it easier for physicians to understand and accept, so that they can make better decisions. The results show that the system has good interpretability, has better acceptance than the common intelligent decision support system, and strongly supports physician auxiliary diagnosis and treatment as well as clinical teaching.
SHIP-MR and Radiology: 12 Years of Whole-Body Magnetic Resonance Imaging in a Single Center
Authors: Hosten Norbert , Bülow Robin , Völzke Henry , Domin Martin , Schmidt Carsten Oliver , Teumer Alexander , Ittermann Till , Nauck Matthias , Felix Stephan , Dörr Marcus , Markus Marcello Ricardo Paulista , Völker Uwe , Daboul Amro , Schwahn Christian , Holtfreter Birte , Mundt Torsten , Krey Karl-Friedrich , Kindler Stefan , Mksoud Maria , Samietz Stefanie , Biffar Reiner , Hoffmann Wolfgang , Kocher Thomas , Chenot Jean-Francois , Stahl Andreas , Tost Frank , Friedrich Nele , Zylla Stephanie , Hannemann Anke , Lotze Martin , Kühn Jens-Peter , Hegenscheid Katrin , Rosenberg Christian , Wassilew Georgi , Frenzel Stefan , Wittfeld Katharina , Grabe Hans J. , Kromrey Marie-Luise
Published: December 2021

The Study of Health in Pomerania (SHIP), a population-based study from a rural state in northeastern Germany with a relatively poor life expectancy, supplemented its comprehensive examination program in 2008 with whole-body MR imaging at 1.5 T (SHIP-MR). We reviewed more than 100 publications that used the SHIP-MR data and analyzed which sequences already produced fruitful scientific outputs and which manuscripts have been referenced frequently. Upon reviewing the publications about imaging sequences, those that used T1-weighted structured imaging of the brain and a gradient-echo sequence for R2* mapping obtained the highest scientific output; regarding specific body parts examined, most scientific publications focused on MR sequences involving the brain and the (upper) abdomen. We conclude that population-based MR imaging in cohort studies should define more precise goals when allocating imaging time. In addition, quality control measures might include recording the number and impact of published work, preferably on a bi-annual basis and starting 2 years after initiation of the study. Structured teaching courses may enhance the desired output in areas that appear underrepresented.
The Impact of Endoscopy Sedation Information Sheets on the Level of Concern Regarding Possible Awareness in Patients Undergoing Endoscopy Sedation
Authors: Tong Zi Ping , Gan Lim Lincoln , Pighills Alison , Hiskens Matthew , Bartlett Danny
Published: December 2021

Sedation encompasses a continuum from complete unconsciousness to drowsiness and anxiolysis where some awareness might be expected. Most patients undergoing endoscopy sedation expect to be completely unconscious during the procedure and thus have unmet expectations regarding their state of consciousness. This study aimed to evaluate whether endoscopy sedation information sheets reduce the level of concern regarding possible awareness during endoscopy sedation at a major regional hospital. Our findings were that 28.8% of patients who received the endoscopy sedation information sheet (n = 82) were concerned about awareness during the procedure, compared to 36.5% of patients in the control group (n = 105). However, the difference was not statistically significant. We also found that the incidence of awareness was higher (13.9%) in the intervention group compared to 8.8% in the control group but, again, not statistically different. This study allowed us to elucidate the level of concern regarding possible awareness during sedation and the incidence of awareness during endoscopy sedation. This will enable future work investigating the role of endoscopy sedation information methods involving written and video material in assisting pre-procedure patient counselling.
Environmental and Fecal Indicator Organisms on Fruit Contact Surfaces and Fruit from Blueberry Mechanical Harvesters
Authors: Holland Renee M. , Chen Jinru , Gazula Himabindu , Scherm Harald
Published: December 2021

Although previous studies have examined microbial loads on food contact surfaces in blueberry packing plants, there is currently no information regarding microbial risks associated with mechanical berry harvesters used in commercial blueberry production. In this study, we surveyed up to nine fruit contact surfaces on seven mechanical harvesters in each of 2015 and 2016 in the field. These surfaces included the shaking rods at the front of the harvester, the sidewalls of the harvesting tunnel behind the shaking mechanism, the catcher plates collecting the detached berries, horizontal and vertical fruit conveyor belts, and berry lugs collecting the fruit at the back of the harvester. Swab samples were collected from each surface three times a day (morning, noon, and evening) and assessed for environmental and fecal indicator organisms including total aerobes, total yeasts and molds, coliforms and fecal coliforms, and enterococci. At the same time points, fruit samples were assessed for microbial loads before the fruit entered each harvester and after they exited the harvester. Results showed statistically significant differences in microbial loads among harvester surfaces, whereas the effect of sampling time was generally not significant. High levels of total aerobes and total yeasts and molds were recorded, especially on horizontal surfaces and/or those located at the bottom of the harvester such as the lower sidewall, the catcher plates, and the horizontal conveyor belt. These surfaces therefore should be targeted by cleaning and sanitization practices. There was also statistical evidence that passage through the harvester may increase the levels of the environmental microorganisms on fruit in the field. In contrast, fecal indicator organisms such as fecal coliforms and enterococci were detected only sporadically and at very low densities on harvester surfaces and blueberry fruit, and there was no evidence that passage through the harvester increased their levels on the fruit. Berry lugs consistently harbored microbial loads, and given their movement back and forth between the field and the packing plant, deserve particular attention with regard to cleaning, sanitization, and storage protocols.
Assessment of Seawater Intrusion in Coastal Aquifers Using Multivariate Statistical Analyses and Hydrochemical Facies Evolution-Based Model
Authors: Hajji Soumaya , Allouche Nabila , Bouri Salem , Aljuaid Awad M. , Hachicha Wafik
Published: December 2021

Groundwater (GW) studies have been conducted worldwide with regard to several pressures, including climate change, seawater intrusion, and water overexploitation. GW quality is a very important sector for several countries in the world, in particular for Tunisia. The shallow coastal aquifer of Sfax (located in Tunisia) was found to be under the combined conditions of continuous drop in GW and further deterioration of the groundwater quality (GWQ). This study was conducted to identify the processes that control GWQ mainly in relation to mineralization sources in the shallow Sfax coastal aquifer. To perform this task, 37 wells are considered. Data include 10 physico-chemical properties of groundwater analyzed in water samples: pH, EC, calcium (Ca), sodium (Na), magnesium (Mg), potassium (K), chloride (Cl), sulfate (SO4), bicarbonate (HCO3), and nitrate (NO3), i.e., investigation was based on a database of 370 observations. Principal component analysis (PCA) and hydrochemical facies evolution (HFE) were conducted to extract the main factors affecting GW chemistry. The results obtained using the PCA model show that GWQ is mainly controlled by either natural factors (rock–water interactions) or anthropogenic ones (agricultural and domestic activities). Indeed, the GW overexploitation generated not only the GWQ degradation but also the SWI. The inverse distance weighted (IDW) method, integrated in a geographic information system (GIS), is employed to achieve spatial mapping of seawater intrusion locations. Hydrochemical facies evolution (HFE) results corroborate the seawater intrusion and its spatial distribution. Furthermore, the mixing ratio showed that Jebeniana and Chaffar–Mahares localities are characterized by high SWI hazard. This research should be done to better manage GW resources and help to develop a suitable plan for the exploitation and protection of water resources.
Quorum Sensing Bacteria in the Phycosphere of HAB Microalgae and Their Ecological Functions Related to Cross-Kingdom Interactions
Authors: Zhang Yanchao , Zheng Li , Wang Shuai , Zhao Yangguo , Xu Xiyuan , Han Bin , Hu Tianyi
Published: December 2021

It has been proven that the relationship between microalgae and bacteria affects the dynamic process of harmful algal blooms (HABs). Microalgae-associated microorganisms widely exist in the phycosphere and play an essential role in algae-bacteria cross-kingdom interactions. Among these processes, quorum sensing (QS), as a communication system of bacteria, is thought to participate in algae-bacteria interactions. However, the species of QS bacteria in the phycosphere and their ecological function are still unknown. In this study, microalgae-associated microorganisms with a QS system were screened by the biosensor method and identified based on 16S rRNA gene analysis. The types and number of acyl-L-homoserine lactone (AHL) signalling molecules produced by QS bacteria were analysed by thin layer chromatography (TLC) bioautography and gas chromatography-mass spectrometer (GC-MS). The film formation, β-dimethylmercaptopropionic (DMSP) degradation and algae growth effects of QS bacteria were investigated. The results showed that 113 QS bacteria were isolated from 842 microalgae-associated bacteria. Detection of AHL molecules in 10 different species of QS bacteria showed that most of them were N-(3-Oxodecanoyl)-L-homoserine lactone (OC10-HSL), N-Octanoyl-L-homoserine lactone (C8-HSL) and N-(3-Oxooctanoyl)-L-homoserine lactone (OC8-HSL). All 10 QS bacteria had film-forming ability, and they could degrade DMSP (except strain E26). The crude metabolic extracts of the 10 QS bacteria can inhibit or promote microalgae growth to different degrees. Our study is helpful to understand the role of microalgae-associated microorganisms with the QS system in algae-bacteria interactions and community succession of HAB microalgae.
The Health Status and Healthcare Utilization of Ethnic Germans in Russia
Authors: Arena Charlotte , Holmberg Christine , Winkler Volker , Jaehn Philipp
Published: December 2021

Ethnic German resettlers from the former Soviet Union are one of the largest migrant groups in Germany. In comparison with the majority of the German population, resettlers exhibit worse subjective health and utilize fewer preventive measures. However, there is little evidence on health among ethnic Germans who remained in Russia. Hence, the objective of this study was to determine the differences in subjective health, diabetes, smoking, and utilization of health check-ups between ethnic Germans and the majority population in Russia. We used data from the Russian Longitudinal Monitoring Survey II from 1994 to 2018 (general population of Russia n = 41,675, ethnic Germans n = 158). Multilevel logistic regression was used to calculate odds ratios (ORs) adjusted for age, sex, period, and place of residence. Analyses were furthermore stratified by the periods 1994–2005 and 2006–2018. Ethnic Germans in Russia rated their health less often as good compared with the Russian majority population (OR = 0.67, CI = 0.48–0.92). Furthermore, ethnic Germans were more likely to smoke after 2006 (OR = 1.91, CI = 1.09–3.37). Lower subjective health among ethnic Germans in Russia is in line with findings among minority populations in Europe. Increased odds of smoking after 2006 may indicate the deteriorating risk behavior of ethnic Germans in Russia.
Remote Health Monitoring in the Workplace for Early Detection of COVID-19 Cases during the COVID-19 Pandemic Using a Mobile Health Application: COVIDApp.
Authors: Echeverría Patricia , Puig Jordi , Ruiz José María , Herms Jordi , Sarquella Maria , Clotet Bonaventura , Negredo Eugenia
Published: December 2021

Background: COVIDApp is a platform created for management of COVID-19 in the workplace. Methods: COVIDApp was designed and implemented for the follow-up of 253 workers from seven companies in Catalonia. The assessment was based on two actions: first, the early detection and management of close contacts and potential cases of COVID-19, and second, the rapid remote activation of protocols. The main objectives of this strategy were to minimize the risk of transmission of COVID-19 infection in the work area through a new real-time communication channel and to avoid unnecessary sick leave. The parameters reported daily by workers were close contact with COVID cases and signs and/or symptoms of COVID-19. Results: Data were recorded between 1 May and 30 November 2020. A total of 765 alerts were activated by 76 workers: 127 green alarms (16.6%), 301 orange alarms (39.3%), and 337 red alarms (44.1%). Of all the red alarms activated, 274 (81.3%) were activated for symptoms potentially associated with COVID-19, and 63 (18.7%) for reporting close contact with COVID-19 cases. Only eight workers (3.1%) presented symptoms associated with COVID-19 infection. All of these workers underwent RT-PCR tests, which yielded negative results for SARS-CoV2. Three workers were considered to have had a risk contact with COVID-19 cases; only 1 (0.4%) asymptomatic worker had a positive RT-PCR test result, requiring the activation of protocols, isolation, and contact tracing. Conclusions: COVIDApp contributes to the early detection and rapid activation of protocols in the workplace, thus limiting the risk of spreading the virus and reducing the economic impact caused by COVID-19 in the productive sector. The platform shows the progression of infection in real time and can help design new strategies.