bluuebunny/crossref_metadata_2025 huggingface dataset:
FREQUENCY OF OBESITY AND METABOLIC SYNDROME IN CHILDHOOD LEUKEMIA AND LYMPHOMA SURVIVORS
Authors: Kartal Ibrahim , Alaçam Abdurrahman , Dağdemir Ayhan , Kara Cengiz , Dinçer Oğuz Salih , Albayrak Canan
Published: January 2024

Objectives: In this study, it was aimed to determine the prevalence and
clinical features of obesity and metabolic syndrome, which are long-term
effects of survivors after treatment in children with leukemia and
lymphoma. Patients&Method: Patients with leukemia and lymphoma, who
were diagnosed between 2000 and 2012 (at least 2 two years after
remission) were included. Data obtained through reviewing the family
history, demographic characteristics, anthropometric measurements, and
laboratory parameters (blood glucose, lipid, and insulin levels) were
analyzed and compared at the time of diagnosis, after the treatment and
at time of the study. Results: Eighty nine patients (45 boys, 44 girls)
were included (mean age: 14.7 ± 4.3 years): 77.5% had acute
lymphoblastic leukemia, 11.2% had acute myeloid leukemia, and 11.2%
had lymphoma. Overall, 46% patients had received radiotherapy, 7% had
undergone surgery, and 2.2% had received stem cell transplantation in
addition to chemotherapy. The mean duration of treatment was 2.4 years,
and the time elapsed after treatment was 4.9 years. While only one had
obesity at the diagnosis, a significant increase in obesity (20%),
hypertension (15.7%), hyperglycemia (15%), insulin resistance (35%)
were observed at the time of study, and family history of hypertension,
dyslipidemia, and cardiovascular diseases were significantly higher in
this subgroup. Conclusion: The prevalence of metabolic syndorme is
higher in children with leukemia and lymphoma after treatment, and
begins to increase with the initiation of treatment and continues to
increase over time. These children should be followed-up for
late-effects including metabolic syndrome through life-long period.
Purification and biochemical characterization of a new thermo-stable laccase from Enterococcus faecium A2 by a three-phase partitioning method and investigation of its decolorization potential
Authors: Birge Aybüke , Alcicek Esra , Baltaci Mustafa Ozkan , Şişecioğlu Melda , Adiguzel Ahmet
Published: January 2024

Three-phase partitioning (TPP) is a simple, fast, cost-effective, and
highly efficient process that can be used in the purification of
laccases. In this study, microorganisms with laccase enzyme activity
were isolated from water samples collected from the Agri-Diyadin
hotspring. The isolate with the highest laccase activity was the A2
strain. As a result of molecular (16S rRNA sequence) and conventional
(morphological, biochemical and physiological) analyses, it was
determined that the A2 isolate was 99% similar to Enterococcus faecium
(Genbank number: MH424896). The laccase was purified to 0.95-fold with
64.59% recovery using the TPP. The molecular mass of the enzyme was
found by SDS-PAGE to be 50.11 kDa. Optimum pH 8.0 and optimum
temperature for laccase enzyme were determined as 80 °C. It was
determined that the enzyme maintained its activity at a rate of 90%
after 1 h of incubation in the temperature range of 20-90 °C and showed
activity and stability in a wide pH range (pH 3.0-9.0). The enzyme
remained highly stable in the presence of surfactants, and increased its
activity in the presence of organic solvents, Cr2+, Cu2+, and Ag+
metals. The Km and Vmax values of laccase enzyme for ABTS substrate were
0.68 mM and 5.29 μmol mL-1min-1.
Function and therapeutic potential of metformin in multiple diseases
Authors: mu wei
Published: January 2024

Metformin is one of the first-line and widely-used drugs in patients
with T2DM due to its safety profile, clinical efficacy and cheap cost.
It is clearly that metformin has benefits on lowering hyperglycemia and
diabetes-related complications in clinical use. However, the exact
mechanisms of metformin in multiple therapies are still blurred. The
classic effect of metformin is to reduce hepatic glucose production by
inhibiting gluconeogenesis in liver and increase glucose utilization in
peripheral tissues. Metformin targets mitochondrial respiratory chain
complex I to specifically reduce reactive oxygen species generation to
protect cells against oxidative stress-induced cell apoptosis. AMPK
complex is a key factor in the action of metformin; however it is
inconclusive that metformin activate AMPK directly or indirectly.
Furthermore, the liver-centered mechanism of metformin has been
challenged by that of gut microbiota alteration and GLP-1 secretion.
Actually, more and more studies showed that metformin act on gut
microbiota to exert anti-hyperglycemia effect. In addition, new emerging
evidence showed that metformin has off-label function on bone
metabolism, cancer inhibition and lifespan extension; however the
underlying mechanism of such properties of metformin still remains
elusive. Despite most of results are from cells in vitro and model
organisms, clinical trials have been recruited by institutes around the
world to verify the therapeutic effects of metformin in anti-tumor and
anti-aging. Taken together, in this review we provide a new perspective
on metformin and repurpose its novel and promising application in the
therapy of cancer, bone fracture healing and age-related diseases.
Adipocyte: an assistant to breast cancer development and a potential treatment strategy for breast cancer
Authors: Kong Zhaodi , Ren Baoqi , Liu Fengjie , Lan Meng , Li Lihong , Zou Tengteng , Yang Zhenjiang , Cai Tiange , Cai Yu
Published: January 2024

With obesity becoming an epidemic, adipocytes have attracted widespread
attention. Growing studies have shown that the function of adipocytes is
far more than storing energy. As endocrine organs, adipocytes play a
pivotal role in the occurrence and prevention of many diseases.
Especially it can secrete cytokines and mediate chronic inflammation,
making it one of the assistants of tumor development. Breast cancer is
the main cause of cancer death in women, and its development has
experienced proliferation, invasion, migration, metastasis, and drug
resistance. It reports that the mammary gland is rich in adipocytes. And
adipocytes can communicate with breast cancer cells and play a pivotal
role in breast cancer development. It displays that adipocytes promote
the proliferation, metastasis, and drug resistance of breast cancer.
This suggests that focusing on adipocytes may be helpful for tumor
treatment. In this paper, we reviewed the regulation mechanism of
adipocytes on the development of breast cancer and proposed strategies
to treat breast cancer. And this may provide a research basis for the
prevention and treatment of breast cancer.
Whole Pulmonary Lavage: Treating Pulmonary Alveolar Proteinosis At The Time of COVID Pandemic
Authors: Powers Katherine , Avadhanula Vasanthi , Patel Purvesh , Sarkar Pralay , Piedra Pedro , Zarrin-Khameh Neda
Published: January 2024

Pulmonary alveolar proteinosis (PAP) is a rare syndrome due to increased
production or decreased clearance of surfactant in alveoli and terminal
bronchi that cause hypoxemic respiratory insufficiency. Here we present
a patient with past medical history of PAP whose disease was exacerbated
by superimposed COVID-19 pneumonia. He underwent whole pulmonary lavage
(WPL). Evaluation of the viral count of the first and the last lavage of
the left lung showed viral load in the alveolar space dropped by
approximately 10-folds, however the magnitude of the viral load was
substantial in both lavage samples. Whole pulmonary lavage may be used
as a treatment option on patients with COVID pneumonia superimposed on a
pulmonary alveolar proteinosis (PAP) exacerbation.
Development of Social Life Impact for Mother (SLIM) scale at first trimester to identify mothers who need social support during postpartum: a hospital-based prospective study in Japan
Authors: Okamoto Yoko , Doi Satomi , Isumi Aya , Sugawara Junichi , Maeda Kazuhisa , Satoh Shoji , Fujiwara Takeo , Nobuaki Mitsuda
Published: January 2024

Objective: Postpartum social problems, such as postpartum depression and
bonding disorders, are important risk factors for child maltreatment.
Mothers with such problems are known to need social support. The aim of
this study was to develop and validate the Social Life Impact for Mother
(SLIM) scale to identify mothers in Japan who need social support during
postpartum. Design: A hospital-based prospective study. Setting:
Obstetric clinics and hospitals in four populous prefectures in Japan.
Sample: A total of 7462 pregnant women. Methods: The participants
completed the SLIM scale at first trimester, and postpartum social
problems (postpartum depression and bonding disorders) were assessed at
one month after delivery (N=5768, follow-up rate: 77.3%). Multivariate
logistic regression was applied to investigate the association between
the SLIM scale and postpartum social problems. Main outcome measures:
Postpartum social problems (postpartum depression and bonding disorders)
at one month after delivery. Results: The SLIM scale predicted
postpartum social problems in moderate accuracy (AUC=0.63, 95%
confidence interval: 0.60-0.65). Further stratification by local clinic
and tertiary hospital did not affect the estimates. Conclusion: The SLIM
scale at prenatal checkup may be useful for obstetricians to detect
mothers with postpartum social problems. Further intervention study
using SLIM score is warranted.
Improvement of interlukin-18 aggregation and activity by replacement of surface cysteine by serine
Authors: Saetang Jirakrit , Roongsawang Niran , Sangkhathat Surasak , Voravuthikunchai Supayang , Sangkaew Natnaree , Prompat Napat , Srichana Teerapol , Tipmanee Varomyalin
Published: January 2024

Interleukin-18 has been proposed for cancer immunotherapy for a long
time. However, the presence of IL-18 binding protein (IL-18BP) and the
unstable form of IL-18 caused the low impact of this protein in human
clinical trials. To overcome this, we performed the mutagenesis
targeting surface cysteines (C38, C68, C76, and C127) on our modified
IL-18 to prevent intermolecular disulfide bond formation. The ORF of
wild-type, IL-18 DM and IL-18 DM1234 were synthesized and expressed in
E. coli. All IL-18 were refolded by step-wise dialysis and tested for
protein aggregation by ProteoStat protein aggregation assay. All
recombinant IL-18 were also investigated for activity by IFN-γ induction
assay. The structure of modified IL-18 was visualized by molecular
dynamic (MD) simulation. The results showed that IL-18 DM1234
demonstrated the lowest aggregation signal compared to others. This
protein also displayed higher activity than wild-type and IL-18 DM about
10 and 2.8 times, respectively. MD simulation revealed the binding site
I of IL-18 DM1234 was the part mostly affected by C-to-S substitution.
In conclusion, this is the first report for IL-18 that the modifications
by mutagenesis improved both activity and stability and this IL-18 may
be used for medical purposes in the future.
An ACP bi-functional tag enhances TEV protease stability and facilitates its covalent immobilisation
Authors: Li Xuefeng , Huang Jiahua , zheng Yujiao , Zhou Junjie , Sun Changsheng , Wang Yuan , wang shengbin
Published: January 2024

Fusion expression is widely employed to enhance the solubility of
recombinant proteins. However, removal of the fusion tag is often
required due to its potential impact on the structure and activity of
passenger proteins. Tobacco etch virus protease (TEV) is widely used for
this purpose due to its stringent sequence recognition. In the present
work, fusing to acyl carrier protein (ACP) significantly increased the
yield of recombinant soluble TEV, and the ACP tag also greatly improved
TEV stability. The cleavage activity of TEV was not affected by the ACP
fusion tag, and ACP-TEV retained high activity, even at unfavourable pH.
Moreover, ACP-TEV could be efficiently modified by co-expressed holo-ACP
synthase (AcpS), leading to covalent attachment of the
4’-phosphopantetheine (4’-PP) group to ACP. The long, flexible 4’-PP
(sulfhydryl group) displayed high specific reactivity with iodoacetyl
groups on the solid support. Thus, TEV could be immobilised effectively
and conveniently via the active holo-ACP, and immobilized TEV retained
high cleavage activity after a long storage period and several cycles of
reuse. As a low-cost and recyclable biocatalyst, TEV immobilised by this
method holds promise for biotechnological research and development.
The natural product berberine synergizes with osimertinib preferentially against MET-amplified osimertinib-resistant lung cancer via MET inhibition
Authors: Chen Zhen , Vallega Karin , Chen Haiying , Zhou Jia , Sun Shi-Yong
Published: January 2024

Background and Purpose: Berberine is a natural antimicrobial,
anti-inflammatory product used in traditional Chinese medicine.
Osimertinib is the first third-generation EGFR-tyrosine kinase inhibitor
(TKI) approved for non-small cell lung cancer (NSCLC) with activating
EGFR mutations and those resistant to earlier generation EGFR-TKIs.
However, emergence of acquired resistance limits its long-term efficacy.
One known mechanism of acquired resistance to EGFR-TKIs is MET (c-MET)
gene amplification. This study has demonstrated berberine’s potential in
overcoming acquired resistance to osimertinib. Experimental Approach:
Drug effects in vitro were evaluated through cell counts, colony
formation, apoptosis and protein degradation assays, and gene
overexpression/knockouts. In vivo drug effects were measured with mouse
xenograft models. Berberine’s interactions with MET were determined via
molecular docking analysis and protein kinase assays. Key Results:
Berberine combined with osimertinib synergistically and selectively
decreased the survival of several MET-amplified osimertinib-resistant
EGFR mutant NSCLC cell lines with enhanced induction of apoptosis,
likely through Bim elevation and Mcl-1 reduction. Importantly, this
combination effectively suppressed the growth of MET-amplified
osimertinib-resistant xenografts in nude mice and was well tolerated.
Molecular modeling showed that berberine could bind to the kinase domain
of non-phosphorylated MET, occupy the front of the binding pocket, and
interact with the activation loop, similar to other known MET
inhibitors. The MET kinase assay clearly showed berberine’s
concentration-dependent inhibition of MET activity. Conclusions and
Implications: These findings collectively suggest that berberine can act
as a naturally-existing MET inhibitor to synergize with osimertinib in
overcoming osimertinib acquired resistance caused by MET amplification.
rhFGF18 restrains osteoporosis caused by estrogen deficiency through promoting MC3T3-E1 proliferation and attenuating oxidative stress-induced osteoblastic apoptosis
Authors: Tian Haishan , Lu Pan , Zhang Meng , Feng Yong , Huang Mai , Shao Ming , Hu Jia , Ding Chao
Published: January 2024

The aim of the present study is to investigate the effect and the
underlying mechanism of recombinant human fibroblast growth factor 18
(rhFGF18) on postmenopausal osteoporosis. The effect of rhFGF18 on the
proliferation of osteoblastic cell line MC3T3-E1 and its related
mechanism were examined. The oxidative stress model of MC3T3-E1 was
established using hydrogen peroxide to evaluate the protective effect of
rhFGF18. Flow cytometry was employed to detect cell apoptosis, while the
protein expressions of Bcl-2, Bax and caspase-3 were detected by Western
blotting. Furthermore, we performed ovariectomy (OVX) on ICR mice to
imitate estrogen-deficiency postmenopausal osteoporosis. Bone metabolism
and bone morphological parameters in OVX mice were evaluated. Our in
vitro study showed that rhFGF18 promoted MC3T3-E1 cells proliferation by
activating extracellular signal-regulated kinase (ERK) and p38 instead
of c-Jun N-terminal kinase (JNKs). rhFGF18 prevented cell damage from
oxidative stress via the inhibition of apoptosis. After rhFGF18
administration, the expression level of anti-apoptotic protein Bcl-2 was
upregulated, whereas pro-apoptotic proteins Bax and caspase-3 were all
downregulated. Administrating rhFGF18 also improved bone metabolism and
bone morphological parameters in OVX mice. Thus, rhFGF18 can effectively
prevent bone loss in OVX mice by enhancing osteoblastogenesis and
protects osteoblasts from oxidative-stress-induced apoptosis.
Novel post-translational modifications of human serum albumin
Authors: Kannan Surya , Krishnankutty Roopesh , Souchelnytskyi Serhiy
Published: January 2024

Serum albumin is the most abundant protein in plasma, has many
physiological functions, and is in contact with most of the cells and
tissues of the human body. Post-translational modifications (PTMs) may
affect functions, stability, and localization of albumin. Here we report
the identification of 61 novel PTMs of human serum albumin (HSA).
Phosphorylation, glycosylation, nitrosylation, deamidation, methylation,
acetylation, palmitoylation, geranylation, and farnesylation are
examples of the identified 31 types of PTMs. Mass spectrometry was used
for the identification of PTMs in a purified HSA and HSA from human
plasma. Three-dimensional modeling of albumin with selected PTMs showed
the location of these PTMs in the regions involved in interactions of
the albumin with drugs, metals, and fatty acids. The location of PTMs in
these regions may modify the binding capacity of albumin. This report
adds 61 novel PTMs to the catalog of human albumin.
Disseminated Salmonellosis and cutaneous vasculitis in a child with IL12Rß1 deficiency – a case report & review of literature.
Authors: Mohite Rachna Shanbhag , KG Rajeshwari , MN Vidya , Bhattad Sagar
Published: January 2024

Mendelian susceptibility to mycobacterial disease (MSMD) is a group of
genetic disorders characterized by a defect in interferon γ (IFN
γ)–mediated immunity, with predisposition to infections caused by
atypical and low virulent mycobacteria. We describe a 10-year-old boy,
presented with left inguinal and submandibular lymphadenopathy with low
grade fever for 10 days. Past history was significant and at the age of
4 months and 5 years, he was diagnosed to have tubercular axillary
lymphadenitis and was treated with anti-tubercular drugs on each
occasion. On examination, he had left submandibular and inguinal
lymphadenopathy and mild splenomegaly. He underwent left inguinal lymph
node biopsy that reported granulomatous lymphadenitis and culture
reported growth of Salmonella enterica. Genetic evaluation showed
pathogenic homozygous mutation of IL12Rß1 gene. A diagnosis of MSMD was
established and he was treated with antibiotics to which he responded
promptly. A detailed review of literature on IL12RB1 deficiency was
performed. Index case has been discussed in light of the previously
published literature.
Interpregnancy Weight Change Among Mothers of a Child with a Major Congenital Anomaly: A Danish Nationwide Cohort Study
Authors: Cohen Eyal , Szentkúti Péter , Horvath-Puho Erzsebet , Brown Hilary , Grandi Sonia , Sørensen Henrik , Ray Joel
Published: January 2024

Objective: To compare the interpregnancy weight gain in women whose
first infant had a major congenital anomaly vs. those without an
affected child. Design: Nationwide cohort study Setting: Denmark
Population: All primigravid women with two consecutive singleton births
between January 2004 and December 2017. Main Outcome Measure:
Inter-pregnancy weight difference Methods: Multivariable linear
regression compared women whose infant had an anomaly vs. those whose
infant did not, adjusting for interpregnancy time interval,
demographics, smoking and health status at the first pregnancy. Results:
Of the 199,536 women, 4035 (2.0%) had a child with an anomaly at the
first birth. The mean (SD) maternal BMI at the start of the first
pregnancy was 24.2 (4.9) and 23.8 (4.6) kg/m2 in women with, and
without, an anomaly-affected newborn. By the start of the second
pregnancy, their interpregnancy weight gain was 4.7% and 4.2%,
respectively – an adjusted absolute higher gain of 0.34% (95% CI 0.11
to 0.58) or 0.13 kg (95% CI 0.01 to 0.26) in women with an
anomaly-affected first-born infant. Compared to those with an unaffected
pregnancy, the interpregnancy weight gain difference was greatest in
women whose first-born infant had a multi-organ anomaly (0.73%, 95% CI
-0.10 to 1.57). Weight gain was more pronounced in women whose infants
spent >30 days in hospital between pregnancies.
Conclusions: Mothers of an infant with a major congenital anomaly have
higher weight gain after pregnancy. Strategies are needed to support a
healthy lifestyle in these women.
Photosynthetic induction upon transfer from low to high light is affected by leaf nitrogen content in tomato
Authors: Sun Hu , Zhang Yuqi , Zhang Shi-Bao , Huang Wei
Published: January 2024

The response of photosynthetic CO2 assimilation to changes of
illumination affects plant growth and crop productivity under natural
fluctuating light conditions. However, the effects of nitrogen (N)
supply on photosynthetic physiology after transition from low to high
light are seldom studied. To elucidate this, we measured gas exchange
and chlorophyll fluorescence under fluctuating light in tomato (Solanum
lycopersicum) seedlings grown with different N conditions. After
transition from low to high light, the induction speeds of net CO2
assimilation (AN), stomatal conductance (gs) and mesophyll conductance
(gm) delayed with the decline in leaf N content. The times to reach 90%
of maximum AN, gs and gm were negatively correlated to leaf N content.
This delayed photosynthetic induction in plants grown under low N
concentration was mainly caused by the slow induction response of gm
rather than that of gs. Furthermore, the photosynthetic induction upon
transfer from low to high light was hardly limited by photosynthetic
electron flow. These results indicate that decreased leaf N content
declines carbon gain under fluctuating light in tomato. Increasing the
induction kinetics of gm has the potential to enhance the carbon gain of
field crops grown in infertile soil.
Population pharmacokinetics and covariate analysis of anlotinib in patients with malignant tumors
Authors: Xu Gaoqi , Yang Dihong , Zhu Junfeng , Ding Haiying , Xin Wenxiu , Zhong Like , Zhu Liqin , Fang Luo
Published: January 2024

Aims Anlotinib is a novel oral tyrosine kinase inhibitor that targets
VEGFR-2, VEGFR -3, FGFR1-4, PDGFR-α, PDGFR-β, c-Kit, and Ret. This study
aimed to develop a population pharmacokinetic model of anlotinib in
patients with malignant tumors and identify various covariates that
might affect its pharmacokinetic parameters. Methods 407 plasma
concentrations from 16 patients were analyzed to establish a population
pharmacokinetic model using the nonlinear mixed-effects model method.
All patients were administered anlotinib (12 mg) orally, as a single
drug therapy. The potential influence of demographic, hepatic, and renal
function data was investigated using automated stepwise covariate model.
The final model was evaluated using diagnostic goodness-of-fit plots,
bootstrap, and visual predictive check methods. Results A
one-compartment model with first-order absorption and elimination
adequately described anlotinib pharmacokinetics. The population
estimates of apparent total plasma clearance (CL/F) was 9.26 L/h
(29.20% IIV), apparent volume of distribution (V/F) was 2010 L (29.20
IIV), and absorption rate constant (Ka) was 0.64 h-1 (56.70% IIV).
Total cholesterol (TC) was identified as a covariate affecting CL/F,
while alkaline phosphatase (ALP) was found to be statistically
significant for V/F. Conclusion The population pharmacokinetic model
adequately described the pharmacokinetics of anlotinib in patients with
malignant tumors. TC and ALP might have a slight effect on the
pharmacokinetic profile of anlotinib.
Influencing Factors and Prediction Methods of Radiotherapy and Chemotherapy in Patients with Lung Cancer Based on Logistic Regression Analysis
Authors: Liu Yuxia , xu Chang , Xing Chengyan , Chen Mingwei
Published: January 2024

objectives: Logistic regression analysis has widespread applications in
clinical disease diagnosis, but it has not yet been applied to assess
the acceptance of radiotherapy and chemotherapy in patients with lung
cancer. A prediction model was established to investigate the
influencing factors of radiotherapy and chemotherapy in lung cancer.
Methods: A sample was admitted of lung cancer patients to Binzhou
Medical University Hospital stays from January 2020 to June 2021. After
investigating doctors, nurses, patients, managers and conducting expert
demonstration, the questionnaire was formed. The questionnaire was
filled out by the patient or the patient’s family members. The factors
in the questionnaire data of patients accepting and not accepting
radiotherapy and chemotherapy were compared for monofactor analysis, and
the significantly different single factor were analyzed by multifactor
logistic regression analysis, explored the influencing factors of
radiotherapy and chemotherapy in lung cancer patients established a
predictive model and drew the receiver operating characteristic curve.
Results: The factors of two groups had statistically significant
differences or no statistically significant differences. After
multifactor logistic regression analysis was conducted, own personality,
self-care ability, disease course classification, own attitude towards
disease treatment, and family attitude towards disease treatment were
included in the influencing factors of radiotherapy and chemotherapy in
patients with lung cancer. Then, a predictive model was established. The
area under the ROC curve of the predicted model was 0.973, the 95%
confidence interval was 0.952-0.995, the optimal critical value was
0.832, the sensitivity was 91.84%, the specificity was 89.09%, and the
accuracy was 90.85%. Conclusions: Based on logistic regression
analysis, the prediction model could predict the extent of accepting
radiotherapy and chemotherapy in patients with lung cancer.
Understanding the factors related to patients with lung cancer accepting
radiotherapy and chemotherapy could provide useful information for the
targeted and effective treatment by clinicia
IgE and High Affinity IgE Receptor in Chronic Inducible Urticaria, pathogenic and management relevance
Authors: Arnau Ana Giménez , Ribas-Llauradó Clara , Mohammad-Porras Nasser , Deza Gustavo , M Ramon Pujol , Gimeno Ramon
Published: January 2024

BACKGROUND: IgE and High-affinity IgE receptor (FcεRI) expression on
basophils has been scarcely explored in patients with chronic inducible
urticaria (CIndU). OBJECTIVES: To investigate baseline serum IgE and
FcεRI expression on blood basophils in a large cohort of CIndU patients
and its relationship to treatment response. METHODS: Baseline total
serum IgE and basophil FcεRI expression measured by flow cytometry in
165 patients with CIndU was studied. The relationship of both parameters
with the response to antihistamine and anti-IgE (omalizumab) treatment
was considered in a subsample of CIndU patients. FcεRI expression in
basophils was assessed by mean fluorescence intensity (MFI) and basophil
FcεRI standardized density (receptors/cell). RESULTS: The median FcεRI
expression standardized per density in blood basophils was found
significantly higher in patients with CIndU compared to HCs. A positive
correlation was found between IgE serum levels and basophil FcεRI
expression. Basal FcεRI expression was not related to antihistamine
treatment response. However, it was related to omalizumab, and patients
responding to omalizumab showed higher basal basophil expression of
FcεRI levels. Non-responders to the antihistamine showed significantly
higher IgE serum levels. CONCLUSIONS: FcεRI receptor overexpression in
patients with CIndU shows almost the same pattern than Chronic
Spontaneous Urticaria. It seems to be independent of CIndU subtypes.
Although additional studies would be welcome, our work highlights the
relevance of FcεRI receptor regulation in CIndU supporting the
autoimmune pathogenesis and suggest that CIndU patients benefit from
anti-IgE therapy.
Transaminitis as a predictor of poor clinical outcomes in pregnant patients infected with COVID-19: retrospective case series
Authors: Youssef Julia , Weingarten Sarah , Fakas Steliana , Kumaraswami Sangeeta , Rajendran Geetha
Published: January 2024

Objective: The objective of this study is to identify the effects of
Coronavirus Disease 2019 (COVID-19) on liver enzymes in pregnant
patients and to determine the significance of transaminitis as a
predictor of worse clinical outcomes. Design: Case series. Setting:
Tertiary care medical center. Population: 31 pregnant patients with a
positive COVID-19 polymerase chain reaction (PCR) test and 20 infants
who presented during March 25, 2020, to May 26, 2020. Methods: Maternal
and neonatal outcomes were compared between patients with transaminitis
and without transaminitis using one tailed Mann-Whitney test for
nonparametric data and the fisher’s exact test for categorical data.
Main outcome measures: Worse maternal outcomes between the groups were
defined as need for intubation, oxygen supplementation, intensive care
unit (ICU) admission, maternal death, and length of hospital stay.
Results: Of the 31 patients with COVID-19, 10/29 (34.5%) were found to
have transaminitis. The patients with transaminitis had increased oxygen
requirements, increased rate of intubation and ICU admissions. Neonatal
outcomes in patients with transaminitis included earlier gestational age
at delivery and lower birth weights, but neither were statistically
significant. Conclusion: Our study revealed that patients with COVID-19
and concomitant transaminitis had worse clinical outcomes in the mother
when compared to those without transaminitis. Further studies should be
conducted to explore this link between abnormal liver studies and poor
outcomes in pregnant patients diagnosed with COVID-19. Keywords:
Coronavirus, COVID-19, transaminitis, pregnancy Tweetable abstract:
Pregnant patients with COVID-19 and transaminitis had worse outcomes
than ones without transaminitis.
Different wave structures for a new extended shallow water wave equation in (3+1) dimension
Authors: Liu Jian-Guo , Zhu Wen-Hui
Published: January 2024

In this work, a new extended shallow water wave equation in (3+1)
dimensions is studied, which represents abundant physical meaning in
nonlinear shallow water wave. We discuss the interaction between lump
wave and single solitary wave, which is an inelastic collision. Further,
the interaction between lump wave and two solitary waves, and the
interaction between lump wave and periodic wave are also studied.
Finally, the interaction among lump, periodic and one solitary waves is
investigated. The dynamic properties of the obtained results are shown
and analyzed by some three-dimensional images.
Influence of cancer in pregnancy on obstetric and neonatal outcomes: An observational retrospective cohort study
Authors: Huang Xuan , Zhang Chen , Zhu Jialei , Li Yueyan , Tang Jing
Published: January 2024

Objective: Determine the effects of cancer treatment in pregnancy.
Design: Observational retrospective cohort study. Setting: 7 Chinese
tertiary A hospitals during 2003–2021. Population: Pregnant patients
with cancer. Methods: We conducted multiple logistic regression to
determine the influence of various factors on preterm birth and
small-for-gestational-age infants, log-binomial regression to analyze
temporal changes, and chi-square tests to explore the effects of cancer
type/treatment. Main outcome measures: Obstetric outcomes; Neonatal
outcomes. Results: Of 204 women, 17% terminated pregnancies; 59%
received pre-delivery treatment. Rates of pregnancy termination
([RR]: 0.48, 95% confidence interval [CI]: 0.35–0.67) and
iatrogenic preterm births (RR: 0.73, 95% CI: 0.54–0.98) reduced, and
that of pre-delivery treatment increased, mainly due to increased rates
of surgery (RR: 1.87, 95% CI: 1.31–2.67). Maternal systemic diseases
were related to a higher risk of small-for-gestational-age infants
([OR]: 12.02, 95% CI: 1.82–79.43). Chemotherapy with taxanes plus
platinum-based agents was related to adverse obstetric outcomes (OR:
1.87, 95% CI: 1.42–2.46, P < 0.05). Thyroid (OR: 0.36, 95%
CI: 0.22–0.57) and ovarian cancer (OR: 0.70, 95% CI: 0.50–0.98) were
associated with fewer cesarean sections. Thyroid cancer was associated
with fetal growth restriction (OR: 5.21, 95% CI: 1.21–22.55).
Conclusions: Rates of pregnancy termination in cancer have declined.
Taxane plus platinum-based chemotherapy was associated with adverse
obstetric outcomes. Cancer type influenced outcomes. Funding: Special
Project for clinical Research in health industry of Shanghai Municipal
Health Commission; Shanghai Science and Technology Commission. Tweetable
abstract: Cancer in pregnancy on obstetric and neonatal outcomes: higher
risks of adverse obstetric and neonatal outcomes.
Covid-19 Vaccine as a potential triggering factor for Anti-GBM disease, A case report and systematic review
Authors: Ahmed Mohanad , Mohamed Sabah , Sibira Rayan , Eltazi Isra , Al-hussein Hussein , Abdulhadi Ahmad Salih
Published: January 2024

COVID-19 vaccine side effects are still a hot area under study, Many
side effects have been identified, and others are still under study.
Herein, we are reporting a patient who developed Rapidly Progressive
Glomerulonephritis due to anti-GBM disease two days after receiving the
COVI-19 vaccine.
Numerical Approach of Fisher's Equation with Strang Splitting Technique using Finite Element Galerkin Method
Authors: karta melike
Published: January 2024

In the present paper, non-linear Fisher’s reaction-diffusion equation is
solved numerically by using Strang splitting technique with the help of
Galerkin method combined with quadratic B-spline base functions. For
this aim, Fisher’s equation is split into two sub-equations with respect
to time such that one is linear and the other is nonlinear. Galerkin
method using quadratic B-spline finite elements is applied to each
sub-equation. To check correctness and reliability of the presented
approach, we have realized on three numerical examples and have made a
comparison with earlier current studies existing in the literature
calculating the error norms $L_{2}$ and
$L_{\infty}$. The solutions obtained during the
article show that the present method is quite proper for using many
partial differential equations in terms of being easy and convenient to
computer application.
Mucormycosis in the urinary bladder, the devil is in the details: a rare case report
Authors: KUMAR MANJEET , Sharma Sunish , Raina Pamposh , Shamra Kalpana , Chauhan Sanjeev
Published: January 2024

Mucormycosis is a fungal infection involving rhinocerebral, pulmonary,
cutaneous, gastrointestinal, disseminated forms with high morbidity and
mortality with rare involvement of urinary bladder. Diagnosis is made by
examining under a microscope, identifying broad, nonseptate, irregular,
ribbon-shaped hyphae. Timely diagnosis and starting antifungal drugs are
key for successful treatment.
Arbitrary solution of the Schrödinger equation interacting with the superposition of Hulthen with spin-orbit plus adjusted Coulomb potential model
Authors: Thompson Edet , Akpan Ita , Thompson Cherish
Published: January 2024

In this study, we solve the non-relativistic radial part of the
Schrödinger wave equation for the superposition of the Hulthen with
spin-orbit plus adjusted Coulomb potential (SHSC) potential using the
Nikiforov-Uvarov (NU) method for arbitrary states. The Hulthen with
spin-orbit plus adjusted Coulomb (SHSC) potential is the simplest
potential field for a nuclear system and has been used to obtain the
single particle energy spectrum for both nucleon species orbiting a
closed nuclear core. We also obtained in this study the corresponding
single particle normalized wave function expressed in terms of the
Jacobi polynomial. Besides, we obtained two special cases of the energy
spectra for the SHSC potential.
Kappa opioids inhibit the GABA/glycine terminals of rostral ventromedial medulla projections in the superficial dorsal horn of the spinal cord
Authors: Otsu Yo , Aubrey Karin
Published: January 2024

Background and Purpose: Descending projections from neurons in the
rostral ventromedial medulla (RVM) make synapses within the superficial
dorsal horn of the spinal cord that is involved in acute nociception and
the development of chronic pain and itch. In addition, this projection
plays an important role in mediating the analgesic effects of opioids.
However, our knowledge about the spinal synaptic targets of RVM
projections and their modulation by opioids is unknown. Experimental
Approach: We used ex vivo optogenetic stimulation of RVM
descending fibres and whole-cell patch-clamp recordings from superficial
dorsal horn (SDH) neurons to identify the target neurons and to
investigate their descending synaptic inputs. Key Results: We
demonstrate that SDH neurons are targeted by descending GABA/glycine
inhibitory inputs from the RVM, although glycinergic inputs predominate.
These SDH neurons had diverse morphological and electrical properties.
This inhibitory synapse was presynaptically suppressed by the kappa
opioid receptor agonist U69593. By contrast, the mu-opioid receptor
agonist DAMGO inhibited only a subset of RVM-SDH synapses, acting both
pre- and postsynaptically, while the delta-opioid receptor agonist
deltorphin II had little effect. Conclusion and Implications: Developing
reliable and effective alternatives to opioid analgesics requires a
detailed, mechanistic understanding of how opioids interact with
nociceptive circuits. This study selectively and systematically
characterises the synaptic connections between RVM projection neurons
and their SDH targets to advance our knowledge of how this descending
projection is organised and modulated. In addition, it improves our
understanding of how opioids alter spinal pathways involved in the
sensations of pain and itch.
Molecular traits and functional exploration of BES1 gene family in plants
Authors: yin zujun , sun zhenting , zhu weidong , Wang Wei , chen xiugui , Li Yan , Xingzhou Liu , Ye wuwei
Published: January 2024

The BES1 (BRI1-EMSSUPPRESSOR1) gene family is a unique class of
transcription factors that play dynamic roles in the Brassinosteroids
(BRs) signaling pathway. An approach of genome-sequencing in a large
number of plants was employed, which permitted the molecular
characterization and a comprehensive functional study on the BES1 gene
family for their potential roles in the development of agronomic traits
and stress responses. A total of 135 BES1 genes were recognized and
characterized, which were divided into 5 well-conserved subfamilies,
consistent with the topology with statistical support and the profound
duplication knots of BES1 paralogues. BES1 was not found in lower plants
as represented by glaucophyta, rhodophyte and chlorophyta. The spatial
expression profiles of BES1s in Arabidopsis, rice and cotton, as well as
their response to abiotic stresses were analyzed. Overexpression of two
rice BES1 genes, i.e OsBES1-3 and OsBES1-5, promotes root growth under
drought stress. Overexpression of GhBES1-4 from cotton enhanced salt
tolerance in Arabidopsis. Five protein interaction networks were
constructed and numerous genes co-expressed with GhBES1-4 were
characterized in transgenic Arabidopsis. BES1 may have evolved in the
ancestor of first land plants following its divergence from algae.
Overexpression of BES1 gene improved the stress tolerance of plants.
Prophylactic sublingual immunotherapy in mice prevents new sensitizations by induction of bystander tolerance
Authors: Brimnes Jens , Brand Stephanie , Lund Kaare , Rask Carola , Andersen Peter
Published: January 2024

Background: Sublingual immunotherapy (SLIT) is a safe and
effective treatment for allergic diseases. While it is generally
accepted that SLIT-induced tolerance is allergen-specific, it has been
suggested that SLIT may also prevent the development of new
sensitizations or even confer protection towards unrelated allergens.
Methods: In the present study we applied murine models of
systemic sensitization to investigate allergen-specific as well as
bystander tolerance induced by SLIT. Results: We demonstrate
that prophylactic SLIT with Phleum pratense (Phl p) extract leads to
allergen-specific systemic tolerance in mice and that this tolerance is
more efficiently induced via the sublingual route compared to the
peroral and intranasal routes. In addition to antigen-specific
tolerance, SLIT with Phl p extract also induced bystander tolerance, as
demonstrated by downregulated reactivity towards the unrelated protein
ovalbumin (OVA). Bystander tolerance was dependent on the presence of
Phl p extract during OVA sensitization, indicating that co-exposure is
critical for the effect. Finally, by assessing the ability of different
antigens (allergens as well as non-allergens) to induce bystander
tolerance, we observed that the ability to induce bystander tolerance
correlated with the immunogenicity of a given protein.
Conclusion: Prophylactic SLIT with immunogenic proteins is able
to prevent the spred of sensitizations to other antigens. This supports
that allergy immunotherapy, used as primary prevention, may prevent
development of new sensitizations to additional allergens and thereby
influence the allergic march.
Trend of consumption of the antifungal agents fluconazole, voriconazole and itraconazole in an adult ICU from 2009 to 2020 in a university hospital in Salvador-Bahia
Authors: FERREIRA FABIO , Pinto Federico Marilia Marilia , Júnior Aníbal de Freitas
Published: January 2024

Invasive fungal infections have a high morbidity and mortality rate in
Brazil. In this aspect, antifungal agents used in hospital units
represent the only treatment alternative. This work aims to to analyze
the trend of antifungal consumption in an Intensive Care Unit (ICU) for
adults, in a university hospital, in Salvador-Bahia. Retrospective
descriptive study, which evaluated the trend of consumption of
antifungal agents in the adult ICU based on the consumption records of
standardized systemic antifungals that were dispensed to patients over
the period and expressed in Defined Daily Dose (DDD)/100 patients -day.
The most consumed antifungals, in descending order were: Fluconazole
(91.58%; p= 0.1726) voriconazole (7.2%; p=0.9356) and itraconazole
(0.45%). Antifungals were widely used in the adult ICU of the
university hospital and the growing consumption trend demonstrates that
it is necessary to monitor and promote the rational use of these drugs,
since the inadequate and/or excessive use, in addition to burdening the
health system , can increase fungal resistance to the only agents
available to fight fungal infections.
Laplacian and Signless Laplacian Energy of Unitary Cayley Graphs
Authors: C THILAGA , B SARASIJA P
Published: January 2024

The Unitary Cayley graph Xn is a graph with vertex set Zn = {0; 1; :::;
n - 1} and the edge set {ab/a; b in Zn; gcd(a - b, n) = 1}. In this
paper we obtain the eigen values of Laplacian, Signless Laplacian matrix
and Laplacian, Signless Laplacian energy of Uni- tary Cayley Graph Xn.
Epidemiological and Clinical Characteristics of All-age Population with Human Respiratory Syncytial Virus, Beijing, 2015--2019
Authors: Luo Ming , gong cheng , zhang yan , wang xue , liu yang , luo qing , Li Aihua , WANG Yiting , Li Maozhong , dong mei , Huang Fang , Xu Wenbo
Published: January 2024

Background We investigate the epidemiological and clinical
characteristics of respiratory infections with respiratory syncytial
virus (RSV) among the entire population in Beijing, China. Methods
All-age patients with respiratory infection between 2015-2019 were
enrolled from 35 sentinel hospitals in Beijing. Their respiratory
specimens were obtained for testing of 11 respiratory pathogens,
including RSV. Their demographic data and medical record were collected
in standardized forms. Results The RSV epidemic season approximately
occurred between October to March with a peak in December or January and
covered 90% of RSV infections with RSV-A dominated in each year.
Children aged 5 years were at the highest risk of RSV infection
(52.2%), followed by the elderly adults aged ≥60 years (25.2%). While
the elderly patients with RSV were prone to develop dyspnea and
lymphocytopenia than other population segments. Moreover, they had an
elevated rate of hospitalization, an increased rate of ICU admission, an
extended length of hospital stay, and elevated mortality compared to the
<5 years children with RSV infection. Conclusions The young
children was the population with the highest risk for RSV infection
followed by the elderly adults. While the later easily developed a
severe infection and need full consideration when making policy.
A case of Gorlin--Goltz syndrome without the characteristic physical features that was diagnosed after the development of a fifth cancer
Authors: Katayama Daisuke , Inoue Akiko , Kayatani Rishu , Urabe Keisuke , Suzuki Ryo , Takitani Kimitaka , Yoshida Masanori , Kato Motohiro , Ashida Akira
Published: January 2024

We present a case of Gorlin–Goltz syndrome (GGS) in a patient who
developed medulloblastoma, osteosarcoma, myelodysplastic syndrome, basal
cell carcinoma, and odontogenic keratocyst by the age of 19 years. He
had no known family history and no characteristic physical features of
GGS. A frameshift mutation in the PTCH1 gene was found in the oral
mucosa as a low-frequency mosaicism, basal cell carcinoma, and normal
skin by whole-exome sequencing of cancer susceptibility genes. Setting a
therapeutic strategy with regard to second cancer development is
important for pediatric cancer patients who have a background of cancer
predisposition. Advances in comprehensive multigenetic analysis are
anticipated to aid in developing such a strategy.
Iodine Adsorption of Shaped Silicalite-1 Silica Zeolite
Authors: Zhou Jia , Bai Pu , Li Tianchi , Chen Qi , Lan Tian , Liu Fang , Yuan Zhongwei , Zheng Weifang , Yan Wenfu , Yan Taihong
Published: January 2024

The effective adsorption capture of iodine produced in the spent fuel
reprocessing is an effective way to avoid the harm of iodine to the
ecological environment and human health. In the investigation described
herein, a new kind of shaped silicalite-1 all-silica zeolite with a
binder which was for direct industrial application other than power was
synthesized and tested for iodine adsorption performance. Although the
existence of binder in shaped silicalite-1 all-silica zeolite, it still
exhibited high iodine adsorption capacities of 507 mg g-1, which was
superior to inorganic adsorbents such as silver-containing zeolite
mainly used currently, and was comparable with that of power
silicalite-1 reported previously. Moreover, the shaped silicalite-1
all-silica zeolite could be cyclic utilization due to its reversible
adsorption. All these show that the shaped silicalite-1 all-silica
zeolite provides the possibility for the application of iodine
adsorption in industrial post-processing condition
Highly Efficient NO Direct Decomposition over BaMnO3-CeO2 Composite Prepared by the One-Pot Method
Authors: Ji Wenxue , Xie Pingping , Li Yongdan , Zhang Cuijuan
Published: January 2024

Developing high-performance catalyst is of paramount for NO direct
decomposition. Here, composite catalysts BaMnO3-CeO2 were designed and
prepared by the one-pot method, which show high catalytic activity,
oxygen resistance and durability. The NO conversion to N2 of 85.9% at
800 oC is achieved for 5%BaMnO3-CeO2. It exhibits 66.5% activity in
the presence of 10 vol% O2 and runs stable for more than 200 h in 5
vol% O2 at 800 oC. The high performance is originated from the strong
interaction between BaMnO3 and CeO2 components, where CeO2 can
effectively inhibit the sintering of BaMnO3 particles, enhance the redox
activity, mobility of lattice oxygen and NO sorption. The proposed
catalytic mechanism based on diffuse reflectance infrared Fourier
transform spectroscopy and kinetic study reveals that the formation of
N2O* intermediate species is the rate determining step. BaMnO3-CeO2
prepared by the one-pot method can be a promising catalyst candidate for
NO direct decomposition.