bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical outcomes of hepaticojejunostomy and ERCP endoscopic bile duct stent implantation in the treatment of malignant obstructive jaundice: a retrospective analysis
Authors: Liu Zhipeng , Han Ziqiang , Niu Zheyu , Hao Yijie , Yu Yuanzi , Zhu Huaqiang , Zhou Xu , Gao Hengjun , Lu Jun , Yang Faji
Published: December 2024

Purpose
Malignant obstructive jaundice (MOJ) refers to intrahepatic or extrahepatic bile ducts obstruction caused by the compression or infiltrative growth of malignant tumors, followed by cholestasis and jaundice. Hepaticojejunostomy (HJ) and Endoscopic Retrograde Cholangiopancreatography (ERCP) bile duct stent implantation are the common palliative treatment to reduce jaundice. The aim of current research was to investigate which treatment was better for patients with MOJ.
Patients and methods:
A retrospective analysis of 171 patients with distal bile duct obstruction due to malignancy admitted to the Department of Hepatobiliary Surgery and Gastroenterology in our hospital from February 2013 to August 2021. According to different treatment options, patients were divided into ERCP group (n = 64) and HJ group (n = 107). The primary end point was overall survival (OS); the secondary end points were postoperative bilirubin level, hospitalization time, recurrence time of obstructive jaundice, and the occurrence of recent postoperative complications.
Results
In this study, we found that There was no significant difference in the median OS between the ERCP group and the HJ group [mOS: 11.6 (95%CI9.4-13.8) months vs 13.3 (95%CI10.7-15.9) months, P = 0.978]. There was no statistical difference in the effect of reducing jaundice between the two groups (P > 0.05). The average hospitalization time in the ERCP group was 6.67 ± 4.02 days, which was significantly shorter than that in the HJ group (9.56 ± 5.38 days, P < 0.001). There was no significant difference in the total incidence of postoperative complications in the ERCP group compared with the HJ group (21.9% vs 21.5%, P = 0.953). However, pancreatitis often occurred in the ERCP group (P = 0.036). In terms of complications of biliary fistula, it mainly occurred in the HJ group (P = 0.029). Compared with the ERCP group, the recurrence time of obstructive jaundice was longer in the HJ group (P = 0.007).
Conclusion
This study showed that both treatments can effectively reduce bilirubin and improve liver function in patients with distal bile duct obstruction due to malignancy. Considering the long-term effect, HJ is superior to ERCP bile duct stenting.
Evaluation of the treatment response of postoperative anti-thyroglobulin antibody in patients with papillary thyroid cancer after 131I therapy
Authors: Hou Shasha , Tan Jian , Meng Zhaowei , Zhang Guizhi , Wang Peng , He Yajing , Zhang Fuhai , Zheng Wei , Wang Renfei
Published: December 2024

Background: Antithyroglobulin antibody (TgAb) affects the accuracy of thyroglobulin (Tg) detection in papillary thyroid cancer (PTC) patients after 131I therapy. This study aimed to evaluate TgAb trends after 131I therapy and investigate their prognostic value for predicting efficacy.
Methods: This retrospective study included 980 PTC patients who underwent total thyroidectomy and 131I treatment. TgAb levels were measured before and after 131I remnant ablation and 131I therapy. The success rates of 131I remnant ablation, time for TgAb to turn negative, and response to 131I therapy were analyzed based on TgAb trends. The effect and predictive value of TgAb levels and change amplitude (△TgAb) in PTC patients on 131I therapeutic efficacy (CR,PR, and NR) were evaluated.
Results: TgAb-positive patients had lower 131I remnant ablation success rates compared to TgAb-negative patients (55% vs. 75%, P=0.007). After the last 131I remnant ablation, 72.1% of patients turned TgAb-negative within 6 months. Higher initial TgAb levels, presence of Hashimoto's thyroiditis, and longer interval between surgery and 131I ablation were adverse indicators for TgAb turning negative. Patients with TgAb <226 IU/ml before 131I ablation and an interval <62 days between surgery and ablation were more likely to turn TgAb-negative within 6 months. The amplitude of TgAb variation was significantly higher in the effective 131I therapy group compared to the ineffective group (P<0.001).The optimal cutoffs for initial TgAb levels and ΔTgAb to predict 131I therapy efficacy were 473 IU/mL and 62%.
Conclusions: TgAb trends are closely related to the efficacy of 131I therapy in PTC patients. Patients with TgAb <226 IU/ml who achieved a negative TgAb status within six months following the initial 131I remnant ablation exhibited a significantly higher success rate for the initial ablation. 83.6% of patients achieved a negative status within one year following successful 131I residual ablation. A lower initial TgAb levels and a shorter interval between surgery and 131I ablation favor TgAb turning negative. ΔTgAb monitoring can provide a basis for predicting the prognosis of PTC patients after 131I therapy.
Transcriptome-based screening in TARDBP/TDP-43 knock-in motor neurons identifies the NEDD8-activating enzyme inhibitor MLN4924
Authors: Lépine Sarah , Maussion Gilles , Schneider Alexandria , Nauleau-Javaudin Angela , Castellanos-Montiel María José , Ambriz Georgina Jiménez , Spiegelman Dan , Abdian Narges , Franco-Flores Anna Krystina , Haghi Ghazal , Gursu Lale , Chaineau Mathilde , Durcan Thomas
Published: December 2024

A growing body of knowledge implicates perturbed RNA homeostasis in amyotrophic lateral sclerosis (ALS), a neurodegenerative disease that currently has no cure and few available treatments. Dysregulation of the multifunctional RNA-binding protein TDP-43 is increasingly regarded as a convergent feature of this disease, evidenced at the neuropathological level by the detection of TDP-43 pathology in most patient tissues, and at the genetic level by the identification of disease-associated mutations in its coding gene TARDBP. To characterize the transcriptional landscape induced by TARDBP mutations, we performed whole-transcriptome profiling of motor neurons differentiated from two knock-in iPSC lines expressing the ALS-linked TDP-43 variants p.A382T or p.G348C. Our results show that the TARDBP mutations significantly altered the expression profiles of mRNAs and microRNAs of the 14q32 cluster in MNs. Using mutation-induced gene signatures and the Connectivity Map database, we identified compounds predicted to restore gene expression toward wild-type levels. Among top-scoring compounds selected for further investigation, the NEDD8-activating enzyme inhibitor MLN4924 effectively improved cell viability and neuronal activity, highlighting a possible role for protein post-translational modification via NEDDylation in the pathobiology of TDP-43 in ALS.
Nanodiamond-embedded tungsten disulfide nanosheets for electrochemical detection of a bacterial metabolite pyocyanin
Authors: Keerthana S. , Divya K. P. , Rajapriya A. , Viswanathan C. , Ponpandian N.
Published: December 2024

Pyocyanin (PYO) is a distinctive electroactive virulence factor secreted by Pseudomonas aeruginosa, a bacterium implicated in a spectrum of severe human infections, particularly in susceptible and critically ill patients. The rapid and accurate diagnosis of infectious agents is crucial for prescribing effective antibiotics and ensuring successful treatment outcomes in patients facing critical conditions. Due to its redox-active nature, PYO actively participates in electron acceptance and donation processes at the electrode surface, making it well-suited for electrochemical detection. To enhance this detection capability, we utilized a physically cross-linked methodology to embellish the surface of a working electrode with a nanocomposite composed of tungsten disulfide nanosheets supported by nanodiamond (WS2 NSs-ND). This innovative approach was implemented to enhance electron transport, and sample absorption on the electrode surface with a sensitivity of 0.38 µA µM− 1 cmdue to its increased number of active sites. Furthermore, WS2 NSs-ND demonstrated remarkable electrochemical performance when applied to real sample human urine, yielding RSD of ± 2%. This underscores the reliability and robustness of WS2 NSs-ND/GCE for the accurate detection of PYO in diverse biological samples, showcasing its potential for clinical applications.
Status epilepticus induced by etanercept biosimilar: a rare case report
Authors: Wang Yangai , Liu Gang
Published: December 2024

Objective
To emphasizes the likelihood of seizure adverse effects linked to rhTNFR-Fc biosimilar.
Methods
A 74-year-old male patient with rheumatoid arthritis experienced a status epilepticus induced by rhTNFR-Fc, which was diagnosed on the basis of brain magnetic resonance imaging and electroencephalography. Anti-TNF medications, including the etanercept biosimilar rhTNFR-Fc, have been linked to the occurrence of seizures. In the event of a seizure adverse event (AE), it is recommended that the suspected drug be discontinued and anti-epileptic drugs be administered to control the seizure.
Results
The administration of rhTNFR-Fc was terminated, and the seizure was managed through the maintenance of levetiracetam and valproic acid treatment.
Conclusion
The rhTNFR-Fc biosimilar may cause seizure adverse effects in patients without any prior history and signifies the critical importance of a neurological assessment and close clinical monitoring being performed in patients with pre-existing seizure disorders before and during therapy with TNF-α inhibitors.
Assembly of the complete mitochondrial genome of Prinsepia uniflora (Rosaceae): Insights into genomic characterization and phylogenetic analysis
Authors: Ma Lu , Chen Shengwei , Zhang Erdong , An Yajing , Zhang Lei
Published: December 2024

Background
Prinsepia uniflora is a significant medicinal and ecological value shrub. Although the genome of this species has been sequenced at the chromosome scale, reports on the mitogenomes of Prinsepia plants are lacking, substantially impedes further research on this species.
Results
We used PacBio HiFi sequencing to assemble the mitogenome of P. uniflora. The mitogenome was characterized is a circular chromosome, with a total length of 328,095 bp; the genes included 35 protein-coding genes, 18 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. Further, our mitogenome characteristics investigation of the P. uniflora included codon usage, sequences repeats and selective pressure. The results showed that a large number of short repetitive sequences existed in the mitogenome of P. uniflora, the codon usage showed a preference for codons ending in A/T, and the codon use bias of the PCGs was modified by natural orartificial selection. We also identifed 20 large fragments that were transferred from the chloroplast genome to the mitogenome. The collinear analysis of mitochondrial structures between P. uniflora and Rosaceae species indicated that P. uniflora was closely related to Prunus padus. Phylogenetic examination based on ML and BI methods also revealed the evolutionary status of the P. uniflora.
Conclusion
In this study, we assembled and annotated the first mitogenome of the Prinsepia. The subsequent analysis provided us with a basis for further molecular studies on Prinsepia mitogenomes and valuable information on the evolutionary biology of Rosaceae species.
Clinical application of a simplified hysteroscopic LNG-IUD non-suture fixation in the treatment of adenomyosis
Authors: Yu Yi , Cong Qing , Sui Long , Zhang Hongwei , Chen Limei
Published: December 2024

Background
Placement of a levonorgestrel-releasing intrauterine device (LNG-IUD) is an effective treatment forpatients diagnosed with adenomyosis who had a strong desire to preserve the uterus and had no immediate fertility requirements. However, there are also some limitations to the suitability of the LNG-IUD for women with adenomyosis, as adenomyosis causes distortion or enlargement of the uterine cavity. Therefore, the LNG-IUD is more prone to expulsion in patients with adenomyosis. In this study, we aimed to show an original and simplified approach using the hysteroscope for non-suture ﬁxation of LNG-IUD in patients with adenomyosis.
Methods
In this retrospectivecase series approved by the Ethics Committee of Obstetrics and Gynecology Hospital of Fudan University, sixty-two patients diagnosed with adenomyosis who had a strong desire to preserve the uterus and had no immediate fertility requirements in the short term were included in the study. They underwent hysteroscopic non-suture fixation of LNG-IUD. All patients underwent one-year postoperative follow-up to evaluate the LNG-IUD expulsion rate, postoperative efficacy and side effects.
Results
Sixty-two patients with adenomyosis underwent non-suture fixation with LNG-IUD and during the long-term postoperative follow-up, every patient experienced complete remission of their symptoms: a significant decrease in menstrual flow, relief of dysmenorrhea, and improvement in quality of life. Only two patients experienced expulsion of the LNG-IUD at 6 months and 10 months postoperatively, respectively.
Conclusions
Hysteroscopic non-suture fixation of the LNG-IUD can be a more simplified and minimally invasive alternative treatment for patients with a history of LNG-IUD expulsion. The non-suture method greatly reduces the complexity of hysteroscopic fixation and can be performed more efficiently in office.
Efficacy and Safety of Negative Pressure Aspiration in Percutaneous Nephrolithotomy for Infectious Kidney Stones: A Retrospective Study
Authors: Chen Wei , He Hui , Chen Xingfa , Long Qinzhi , Zhang Na , LI Xiang
Published: December 2024

Background
Percutaneous nephrolithotomy (PCNL) is the preferred treatment for large and complex infectious kidney stones but is associated with a risk of urosepsis due to elevated renal pelvic pressure. Negative pressure aspiration during PCNL may mitigate this risk by continuously evacuating irrigation fluid, stone fragments, and bacteria, thereby lowering intrarenal pressure and reducing postoperative complications. This study evaluates the safety and efficacy of PCNL with negative pressure aspiration for treating infectious kidney stones.
Methods
A retrospective study included 126 patients with infectious renal stones treated from October 2017 to May 2024. Among them, 57 underwent conventional mini-PCNL, and 69 received mini-PCNL with negative pressure aspiration. Demographic data and perioperative parameters, including operation time, blood loss, hospital stay, and stone clearance rate (SCR), were recorded. Blood loss was assessed by hemoglobin changes, and inflammatory responses were measured using serum IL-6 and procalcitonin (PCT) levels. Postoperative complications were classified using the Modified Clavien-Dindo Classification system.
Results
Negative pressure aspiration significantly improved primary SCR (79.7% vs. 59.6%) and reduced postoperative fever incidence (8.7% vs. 31.6%) compared to conventional PCNL. The incidence of urosepsis was lower (2.9% vs. 14%), and blood loss was reduced, with fewer transfusions required. Patients treated with negative pressure aspiration recovered faster, resuming normal activities in an average of 2.41 ± 1.35 days compared to 3.05 ± 1.78 days for conventional PCNL patients. The average hospital stay was shorter for patients with negative pressure aspiration (3.44 vs. 4.47 days).
Conclusions
Negative pressure aspiration during PCNL offers significant benefits, including improved stone clearance, reduced postoperative complications, lower infection risk, and faster recovery. This technique should be considered a preferred option for treating infectious kidney stones, demonstrating superior surgical outcomes and enhanced patient recovery compared to traditional PCNL.
The application effectiveness of the “One Case to the End” teaching model based on BOPPPS in Pediatric Nursing: A quasi-experimental study
Authors: Xiao Xuejun , Xie Ying , Zhao Xiangfeng , Dai Xiaohong , Liang Feng , Wang Wanchao , Zhang Xianhua
Published: December 2024

Background/Aim The traditional teacher-centered teaching method of pediatric nursing only focuses on the imparting of knowledge, ignoring the cultivation of students’ autonomous learning abilities and the establishment of critical thinking skills. Moreover, the humanized care for pediatric patients is merely a formality, which can’t make a deep impression. This study aimed to explore the application effectiveness of the “One Case to the End” teaching model based on BOPPPS in pediatric nursing teaching. Materials&methods A quasi-experimental research design was conducted in this study. A total of 276 nursing undergraduates from Grades 2020 and 2021 of a medical college were randomly selected, with 138 students in the Grade 2020 serving as the control group and receiving the traditional teaching model, while the students of Grade 2021 served as the experimental group and implemented the “One Case to the End” teaching model based on BOPPPS. At the end of the semester, the study compared the two groups of students’ academic performance, the level of nurses’ professional values and critical thinking abilities. Additionally, it surveyed the experimental group’s satisfaction with the curriculum teaching reform. Results The academic performance, the score of nurses’ professional values and the score of critical thinking abilities of the experimental group were significantly higher than those of the control group (all P < 0.01), and the students in the experimental group had a relatively high evaluation of the reform satisfaction with the new teaching model. Conclusion The “One Case to the End” teaching model based on BOPPPS can effectively enhance students’ academic performance, nurses’ professional values and critical thinking abilities, and improve the teaching quality of pediatric nursing.
Sex-Related Disparities in Outcomes of Survival in Biliary Tract Cancer Patients
Authors: Sever Nadiye , Yunusov Emil , Majidova Nargiz , Kocaaslan Erkam , Erel Pınar , Ağyol Yeşim , Güren Ali Kaan , Çelebi Abdussamet , Işık Selver , Bayoğlu İbrahim Vedat , Köstek Osman , Sarı Murat
Published: December 2024

Introduction:
Biliary tract cancer (BTC) is a rare and aggressive cancer with a poor prognosis. Despite treatment, overall survival is less than 12 months. It is a proven fact that women have better chemotherapy responses and survival than men in almost all cancer types. We believe that gender is one of the important factors affecting the prognosis of BTC. In this study, we aimed to investigate the effect of gender on prognosis in this type of cancer.
Methods
This study was designed as a single-centre retrospective analysis of patients with BTC. All patients, regardless of operability, were included in the study. Prognostic factors were analysed using univariate and multivariate analysis.
Results
A total of 100 patients (48% female) were included in the study. The median follow-up time was 72.2 months (95% CI 39.3–105.0), and the median OS was 9.5 months (95% CI 5.3–13.8) for all study patients. The 72-month survival rate was 13.4%. The observed survival rates at 10.4% for male patients and 15.7% for female patients demonstrate the importance of considering gender as a prognostic factor. A multivariate analysis indicated a significant association between female gender and longer overall survival, with an adjusted hazard ratio of 0.59 (95% CI 0.38–0.92, p = 0.02).
Conclusion
It is clear that female gender is associated with a better response to chemotherapy and longer survival in BTCs. These findings should be taken into account in treatment selection and prognosis predictions. Further research may help elucidate the mechanisms underlying these sex differences and help develop more effective treatments.
Intratumoral and peritumoral radiomics analysis for prediction of MGMT methylation status in glioblastoma
Authors: Chen Wang-Sheng , Cai Qin-Lei , Wang Fei , Wang Xue-Hua , Fu Fang-xiong , Hong Lan , Su Li
Published: December 2024

Background
The assessment of MGMT promoter methylation is essential for determining the appropriate therapy for glioblastoma. However, prior studies have primarily focused on intratumoral regions, neglecting the peritumoral area. This study aimed to develop a radiomic model utilizing features from both intratumoral and peritumoral regions derived from MRI images.
Methods
The study included 96 glioblastoma patients who were randomly divided into training and testing sets. Radiomic features were extracted from both tumor and peritumor regions. We constructed radiomic models based on intratumoral, peritumoral, and combined features for comparison. The models were evaluated using the area under the receiver-operator characteristic (ROC) curve (AUC).
Results
The combined radiomic model achieved an AUC of 0.814 (95% confidence interval: 0.767–0.862) in the training set and 0.808 (95% confidence interval: 0.736–0.859) in the testing set, surpassing the performance of models based solely on intratumoral or peritumoral features. Additionally, calibration and decision curves indicated excellent model fit and clinical utility.
Conclusions
The radiomics model incorporating both intratumoral and peritumoral features shows promise in differentiating MGMT status, which can inform clinical treatment strategies for glioblastoma.
Impact of a new image enhancement technology on the nuchal translucency thickness
Authors: Elger Tania , Prodan Natalia , Bettecken Kristina , Sonek Jiri , Kagan Karl Oliver
Published: December 2024

Objective: To examine the impact of a new image enhancement technique on the distribution of NT measurements.
Methods: In this retrospective study, nuchal translucency (NT) images that were taken with the GE Voluson E22 ultrasound machine between May and September 2024 were collected. One operator took manual and automated NT measurements in an NT image without radiant enhancement mode followed by automated measurements in NT images with minimum, medium and maximum radiant mode. The automated measurement in the NT images without radiant mode were considered gold standard. The relative bias of the gold standard, the manual measurement and the automated measurements with the radiant enhancement technology were compared using median and 25-75th interquartile range as well as by the Wilcoxon test.
Results: The database search yielded 352 NT images from 101 pregnant women. Average maternal age of the study population was 34.0 years and average crown rump length was 68.3.
The median NT thickness was 1.7 mm for both the manual and automated measurements done without the use of Radiant mode. The median automated measurement with minimum, medium and maximum radiant mode was 2.00, 2.05 and 2.10 mm, respectively. All automated measurements were significantly higher than the gold standard.
Discussion: The use of the radiant mode results in an increase in the NT thickness. This effect should be taken into account when calculating the risk for chromosomal abnormalities.
Incidence of In-Hospital Mortality and Its Determinants among Intensive Care Unit Patients with Acute Respiratory Distress Syndrome in Ethiopia: A multilevel analysis
Authors: Abate Semagn Mekonnen , Kebede Melkamu , Hailu Seyoum , Adamu Yayeh , Mantefardo Bahru , Meno Abinet , Lafebo Lakew
Published: December 2024

Background
Acute respiratory distress syndrome (ARDS) is a heterogeneous syndrome with substantial morbidity and mortality globally. Body of evidence revealed that the epidemiologic estimates are currently disproportional due to differences in patient populations, risk factors, resources, and practice protocols around the world, and the rate of mortality and its predictors are uncertain in Ethiopia.
Method
A multi-centre longitudinal study was conducted in Ethiopia from January 2018 to June 2023. After receiving ethical clearance from the Institutional Review Board (IRB) of Dilla University College of Health Science and Medicine, 356 ARDS patients’ records were retrieved with a systematic random sampling technique. A multilevel multivariate analysis was used to control the effect of clustering. A P < 0.05 was taken as statistically significant.
Results
This study demonstrated that the cumulative mortality rate of patients with ARDS was 59% (95% CI: 53.5 to 63.9). The multilevel multivariable model analysis showed that GCS < 8 (AOR = 7.4; 95% CI: 2.79, 19.75), severe form of ARDS (AOR 4.7 95% CI 1.64, 13.36), invasive ventilation (AOR 3.2, 95% CI 1.56, 6.42), and respiratory comorbidity (AOR 4.9, 95% CI 1.71, 14.32) were independent predictors of in-hospital mortality among patients with ARDS.
Conclusion
The study revealed that the hospital mortality rate was substantially higher than that of developed nations. The study also highlighted various risk factors that independently predicted in-hospital mortality.The findings of this study call for mitigating strategies to improve ICU care for ARDS patients.
Effect of Sprouting on the Physicochemical, Antioxidant and Digestive Enzyme Inhibitory Properties of Pro-vitamin a Biofortified Yellow Maize Products
Authors: Emmanuel Ajani O. , Adeyeye Quadri Adeyinka , Emmanuel Irondi A.
Published: December 2024

Background Sprouting is one of the many processing techniques the food industry explores to improve the nutritional quality of cereals and other grains. Pro-vitamin biofortified maize alleviates Vitamin A deficiency and serves as an important source of antioxidants. This study aimed to determine the effect of sprouting on the physicochemical, antioxidant, and digestive enzyme inhibitory properties of pro-vitamin A biofortified maize. The maize grown at the International Institute of Tropical Agriculture (IITA) research field, Ibadan was initially subjected to analysis in which the proximate composition, functional properties, bioactive composition, antioxidant activity, and starch hydrolyzing inhibitory activity were carried out before sprouting. The samples of the sprouted maize were collected for different days (Day 1 to 4) and were further analyzed. Samples were further processed into donkwa, tuwo, and corn pudding using the traditional processing method, then analyzed for bioactive contents and blood glucose response in volunteers. Results obtained were analyzed using the SPSS software package and the mean values were compared.Results In the result, the proximate composition, functional properties, bioactive composition, antioxidant activity, and starch hydrolyzing inhibitory activity of the sprouted samples increased considerably compared to the non-sprouted (control). The proximate composition and digestive enzyme inhibitory activity of the samples increased with an increase in the day of sprouting. The bioactive composition of the day 3 sprouted sample varied significantly when compared with others including the control. The antioxidant activity of day 3 and 4 vary significantly compared to the control. The bioactive components in the products increased in their concentration with an increase in the day of sprouting. The blood glucose response in the volunteered subjects was higher in tuwo than donkwa and corn pudding.Conclusion The sprouting of provitamin A biofortified yellow maize enhanced antioxidants, protein, moisture, carbohydrates, hydrolyzing enzymes, and other essential nutritive substances.
Evaluating Implementation Strategies for Clinical Practice Guidelines in Ambulance Services: A Systematic Review Using the CFIR Framework
Authors: Maria A/Pro Sonja , Brewster Lyle , Gray Brianna
Published: December 2024

Background
Clinical Practice Guidelines (CPGs) are crucial for standardising care, improving quality, and supporting evidence-based decision-making in emergency medical services. However, their implementation in emergency medical services (EMS) is often hindered by diverse challenges, including resource constraints, workforce variability, and organisational differences. Addressing these barriers requires targeted strategies tailored to the unique demands of paramedic environments.
Methods
This systematic review evaluated implementation strategies for CPGs in EMS using the Consolidated Framework for Implementation Research (CFIR) as a guiding structure. Following Joanna Briggs Institute methodology and adhering to PRISMA 2020 guidelines, we conducted a comprehensive search across multiple databases, focusing on studies from the past 20 years. Included studies examined CPG implementation strategies such as training, leadership interventions, and technology adoption. Data were extracted, synthesised, and analysed within CFIR domains to explore the factors influencing adherence and outcomes.
Results
A total of 41 studies met the inclusion criteria, highlighting significant variability in CPG implementation success across regions and contexts. Key barriers included resource dependency, protocol complexity, and resistance to change, particularly in under-resourced and rural settings. Facilitators included adaptable protocols, leadership engagement, and structured training programs. Tailored interventions, such as scenario-based training and collaborative frameworks, demonstrated improved adherence and patient outcomes, with sustainability influenced by organisational readiness and external pressures.
Conclusion
Effective implementation of CPGs in EMS requires multifaceted approaches that address barriers across CFIR domains. Leadership engagement, adaptable protocols, and equitable resource allocation are critical for enhancing adherence and improving patient outcomes. These findings provide actionable insights to inform policy, practice, and future research.
A gerinc egészsége serdülő korú tanulók körében
Authors: Szigethy Mónika , Arany Dániel , Góczán Pál
Published: December 2024

Az iskoláskorú gyermekek körében egyre gyakoribb a helytelen testtartás, amely hosszú távon különböző mozgásszervi problémákhoz vezethet. A növekedés intenzív időszakában a gyermekek törzsizomzata gyakran nem elég erős, amit tovább súlyosbít az ülő életmód és a nem megfelelő iskolai környezet. A tartáshibák és gerincbántalmak prevenciója érdekében kulcsfontosságú a korai felismerés és a megfelelő intervenciós programok alkalmazása.
Jelen kutatás célja, hogy felmérje egy célzott tartásjavító program hatását serdülőkorú tanulók gerincszakaszainak változásaira, és vizsgálja a pandémia alatt bekövetkező otthoni inaktivitás hatását. A kutatás során a Spinal Mouse eszközzel vizsgáltuk serdülő korú gyermekek gerincét, valamint az Idiag Posture Score és a Matthiass teszt eredményeit figyelembe véve értékeltük a kapott eredményeket.
“-(I)yor ol-” BİRLEŞİK FİİL YAPISINDAKİ {-(I)yor} BİR SIFAT-FİİL EKİ MİDİR?
Authors: Fener Kubilay
Published: December 2024

Süreklilik bildiren bir art fiilin geniş zaman çekiminden hece düşmesi sonucu oluşan {-(I)yor} eki, kategori bakımından Türk gramerciliğinin tartışmalı konularından birisini teşkil etmektedir. Kimi araştırmacılar, anılan ekin çeşitli kullanım alanlarından yola çıkarak bir sıfat-fiil olduğunu ileri sürerken bazıları ise bu görüşe katılmamaktadır. Belirtilen duruma istinaden, bu makalede eş zamanlı bakış açısıyla {-(I)yor} ekinin bir sıfat-fiil eki olup olmadığı ele alınacak ve konuya ilişkin görüş ifade eden araştırmacıların düşünceleri kritiğe tabi tutulacaktır. Bununla birlikte ilgili ekin sıfat-fiil kabul edilmesindeki en önemli etkenlerden birisi olan “-(I)yor ol-” yapısının Türkçede hangi etkenlere bağlı olarak ortaya çıktığı ve yaygınlaştığı edebî metinler esasında ortaya konmaya çalışılacaktır.
Idade da doadora e diferentes gêneros receptores: respostas ao xenotransplante ovariano ovino por longo período de cultivo
Authors:   , Santos Fernando Araujo dos
Published: December 2024

O estoque de folículos primordiais e primários existente em ovários fetais representa um potencial inexplorado que pode ser cultivado para fins de reprodução, preservação ou pesquisa. Adicionalmente, a utilização de receptores masculinos imunodeficientes no xenotransplante ovariano fornece mais uma oportunidade de aproveitamento de material genético que poderia ser perdido. Dessa forma, objetivou-se avaliar o desenvolvimento e viabilidade do tecido ovariano ovino, na fase fetal e na fase prépubere, xenotransplantado a fresco, em diferentes linhagens e sexo de camundongos imunossuprimidos. Para o primeiro experimento, ovários de fetos ovinos (100-140 dias) foram colhidos após o abate de suas genitoras, divididos em pequenos fragmentos (1 mm3 ) que foram destinados a avaliação controle e ao xenotransplante a fresco. Foram utilizadas cinco receptoras da linhagem C57BL/6 SCID para o xenotransplante. Cinco dias após o procedimento cirúrgico, as camundongas receptoras foram submetidas ao lavado vaginal durante 60 dias. Ao final desse período foram eutanasiadas e procedeu-se com a coleta dos transplantes para avaliações macro e microscópicas. Observou-se que em 80% das fêmeas receptoras o padrão citológico do lavado vaginal alternou entre diestro-proestro-diestro, não sendo observadas as fases de estro e metaestro durante o período experimental. Na análise histológica foi constatado que a neovascularização ocorreu em 80% dos tecidos recuperados. Observou-se também folículos pré-antrais e antrais (menores) e sem alterações morfológicas, no entanto, não foram obtidos folículos antrais com mais de 1mm. Corpos lúteos também não foram visualizados. No segundo experimento, fragmentos ovarianos (1 mm3 ) de ovelhas jovens foram xenotransplantados a fresco em receptores machos da linhagem C57BL/6 SCID (n = 5) e BALB/c Nude (n = 5). Após 65 dias do xenotransplante, os animais foram eutanasiados e os transplantes avaliados quanto às características macroscópicas, microscópicas e o desenvolvimento oocitário in vitro. Foi observado nos ovários recuperados dos animais da linhagem C57BL/6 SCID grande consumo folicular. Dos folículos morfologicamente normais restantes, a maioria era primordial. Adicionalmente, áreas com tecido cicatricial semelhante ao corpo albicans também foram encontradas. Nos transplantes recuperados dos animais da linhagem BALB/c Nude foi observado consumo folicular moderado, apresentando baixa taxa de folículos primordiais e primários normais e alta proporção de primordiais atrésicos. Adicionalmente, recuperou-se um oócito, o qual foi submetido à maturação, fecundação e cultivo in vitro sem, contudo, apresentar desenvolvimento embrionário. Considerando que utilizou-se doadoras da mesma espécie, conclui-se que a idade da doadora, a espécie receptora e o período para a coleta do tecido ovariano devem ser adaptados para melhores resultados.
Dietary Risk Factors of Nasopharyngeal Carcinoma: A Case-Control Study in Western Algeria
Authors: Boumansour Naouel Fatima Zohra , Kehili Hakima , Bemgueddach Aicha , Boughrara Wefa , Midoun Nori
Published: December 2024

Objective:

Nasopharyngeal carcinoma (NPC) is a prevalent cancer in Algeria, where it ranks as the most common cancer of the aerodigestive tract in men,
with an incidence rate of 5.1 cases per 100,000 persons per year. The etiology of NPC is multifactorial. Thus, this research aims to identify specific
dietary risk factors prevalent in the western Algerian region.

Methods:

We conducted a case-control study comprising 120 incident cases of histologically confirmed nasopharyngeal carcinoma, carefully matched with
120 controls.

Results:

Nasopharyngeal cancer, more common in males (average age 45.2 ± 2.5 years), is linked to consuming traditionally preserved foods (khlii, salted
fats, boiled milk, processed meats), elevating NPC risk. Significant associations include hot chili peppers, citrus fruits, and dried vegetables, while
increased fruit and coffee intake reduces risk. High-temperature cooking and fried food show a strong link. Multivariate analysis identifies hot
chilli, citrus fruits, fresh fruits, and coffee as the key factors significantly associated with NPC.

Conclusion:

This research provides essential insights for implementing primary prevention measures to combat nasopharyngeal carcinoma effectively. By
identifying specific dietary risk factors prevalent in the western Algerian region, healthcare authorities can focus on targeted interventions to
reduce the burden of NPC in this population.
Identification of a Ubiquitination‑Related Signature for Ovarian Cancer Based on 101 Combinations of Machine Learning Methods
Authors: He Miao , Guo Yu , Yu Minmin , Lin Changsong
Published: December 2024

Background:

Ovarian cancer is the third most common gynecological cancer worldwide.
The majority of ovarian cancer instances are identified at later stages of progression, resulting in a
significantly elevated mortality rate. Additionally, the absence of reliable prognostic biomarkers
significantly limits the effectiveness of prediction and personalized treatment approaches. Ubiquitination,
a protein modification process intricately associated with diverse tumor-related biological
pathways, occupies a central position within the tumor microenvironment (TME). However, its specific
mechanisms in ovarian cancer remain inadequately understood.

Methods:

In this study, we explored ubiquitination-related genes at both single-cell and bulk transcriptome
levels utilizing algorithms, such as AddModuleScore, single-sample Gene Set Enrichment
Analysis (ssGSEA), and Weighted Gene Co-Expression Network Analysis (WGCNA). We
then designed a novel machine learning framework featuring 10 algorithms and 101 combinations
to establish a Ubiquitination-Related Signature (URS), which was validated in both training and external
datasets. Additionally, we developed a comprehensive nomogram for clinical prognosis based
on the URS. To thoroughly investigate prognostic characteristics, we applied multi-omics data. This
analysis provided a deep understanding of the prognostic signature. Furthermore, we assessed the
response of different risk subgroups to immunotherapy and identified suitable drugs for specific risk
profiles, thereby advancing personalized medicine.

Results:

In this research, we discovered 35 ubiquitination-related genes significantly linked to the
prognosis of ovarian cancer. Our univariate and multivariate assessments indicated that the low-risk
cohort exhibited a better survival rate than the high-risk cohort, suggesting the potential of the model
as an independent prognostic marker. The nomogram based on the URS offers a quantitative tool
for clinical applications and can be effectively utilized in healthcare settings. Moreover, we observed
significant differences in biological functions, mutation patterns, and immune cell infiltration
within the tumor microenvironment between the high-risk and low-risk groups. Additionally,
we highlighted potential drugs tailored for specific risk categories.

Conclusion:

We developed and validated a signature related to ubiquitination for ovarian cancer,
which shows potential as a reliable predictor of prognosis. Investigating ubiquitination paves the
way for innovative clinical strategies and novel anti-tumor therapies.
Expression of CD68+ Cells in Synovial Tissue from Patients with PsA and its Association with Disease Activity Indices: A Clinical Pilot Study
Authors: Popova-Belova Stanislava , Geneva-Popova Mariela , Popova Velichka , Kraev Krasimir
Published: December 2024

Introduction:

Investigating CD68+ positive cells in the synovial tissue is crucial for understanding
the pathogenesis of psoriatic arthritis (PsA) and developing targeted treatment strategies.
The role of CD68+ positive cells in the synovial tissue of patients with PsA for joint destruction
has not been fully studied.

Objective:

The objective of the study was to examine the presence of CD68+ cells in the synovial
tissue of patients with PsA, particularly those with high inflammatory activity.

Methods:

Synovial tissue samples were collected during knee joint replacement surgeries from patients
with PsA (16 patients) and gonarthrosis (25 patients). Immunohistochemical methods were
employed to detect CD68+ cell expression in the tissue samples. The results were analyzed by histologists,
and the staining intensity and percentage of positively stained cells were evaluated. The
data were then divided into three groups for statistical analysis: negative, weakly positive, and
strongly positive histological samples. Routine indices for disease activity, VAS, DAPSA, PASDAI,
and mCPDAI were used to assess PsA activity in all patients and to assess correlations with
CD68+ positive cells in the synovial tissue. Statistical analysis was performed using SPSS version
26.0 (SPSS Inc., Chicago, IL, USA).

Results:

The expression of CD68+ positive cells was significantly higher in patients with PsA compared
to those with activated gonarthrosis (p < 0.001). The indices for disease activity, VAS, DAPSA,
PASDAI, mCPDAI, and mCPDAI showed a significant positive relationship with the expression
of CD68 + cells on synovial tissue in patients with PsA (p < 0.01)

Conclusion:

The findings of the study confirm the increased numbers of CD68+ cells in PsA vs.
gonathrosis synovium. This suggests the need to explore therapeutic approaches aimed at suppressing
or blocking CD68+ cells to potentially mitigate joint damage.
Leveraging Drug-Gene Interaction Information for Personalized Hypertension Drug Molecule Discovery
Authors: Lehasa Odifentse M. , Chude-Okonkwo Uche A.K.
Published: December 2024

Aims:

To examine whether novel lead hypertension molecules can be used in
the personalized treatment of hypertension.

Background:

Hypertension is a modifiable condition that affects over 1 billion adults
worldwide. Maintaining a healthy blood pressure is vital for overall health, especially
given that hypertension is a primary risk factor for developing cardiovascular conditions.
However, some individuals have resistant hypertension, which may be due to variations
in genetic expression, making standard hypertension treatments ineffective.

Objective:

The integration of genetic data into the personalized optimization of novel hypertension
drugs is demonstrated.

Method:

This research created coding criteria for drug-gene recommendations based on
the genomic profiles of ten pseudo-patients. The genomic data of these patients was created
using chromosome and hypertension-implicated gene sequences from the NCBI National
Library of Medicine database.

Results:

This study uses the proposed drug recommendation criteria to recommend novel
hypertension lead molecules to each patient based on their gene expression profiles.

Conclusion:

This study’s patient-centric drug prescription approach integrates patient
gene expression data with drug-gene interaction data and recommends novel hypertension
drugs most suitable for each patient. Variations in patient gene expression explain the diverse
treatment responses inherent across hypertensive patients, thus necessitating a personalized
approach to their drug prescription. Future studies can investigate the challenges
of ethical, technological, and technical expertise that may affect the clinical implementation
of personalized drug prescription recommendation systems.
Convolutional Neural Network-based Smart Disaster Management Framework for Real-time Detection and Management of Forest Fires
Authors: Singh Kuldeep , Sharma Eshaan , Baath Puneet Kaur , Kaur Kiranbir , Singh Harminder
Published: December 2024

Introduction:

Timely detection of catastrophic natural disasters, such as forest fires, is
critical to minimizing losses and ensuring rapid response. Artificial intelligence is increasingly being
recognized as a valuable tool in enhancing various stages of disaster management.

Method:

This paper presents the development of a smart framework utilizing machine learning
techniques for real-time detection and monitoring of natural disasters, specifically forest fires. The
proposed approach employs a 10-layer convolutional neural network (CNN) that classifies aerial
images into Fire, Non-Fire, and Smoke categories with high precision and speed. In addition to this,
a CNN-based feature extraction process is performed and integrated with various ML classifiers,
including support vector machine, k-nearest neighbor, decision tree, random forest, and extra trees.

Results:

Extensive performance analysis reveals that the proposed 10-layer CNN model outperforms
other classifiers, achieving an accuracy of 97.64% in the binary classification of fire vs. nonfire
and 95.61% in the three-class classification of Fire, Non-Fire and Smoke classes. Furthermore,
a comparative study with existing state-of-the-art methods demonstrates the proposed model's superior
performance in both accuracy and computational complexity.

Conclusion:

These results demonstrate the potential of the proposed CNN-based framework to
serve as a reliable and effective tool for real-time disaster management across various applications,
providing valuable support to emergency response teams in mitigating the impact of natural disasters.
BLE-IOT-PID: Bluetooth Low Energy (BLE) Based IOT Controlled PID Controller for Multi-loop Pilot Plant with Quantum Firefly PSO Optimization
Authors: Deshmukh Jotiram , Tirole Ritesh , Bhaskar Anand
Published: December 2024

Introduction:

Conventional Proportional–integral–derivative (PID) controls for multiloop
pilot plants are constrained by wired connections, outdated control techniques, and inefficient
real-time data sensing and acquisition. As a result, inefficiencies arise, where control loops for parameters
like temperature, level, and flow require continuous dynamic adjustments and precise regulation.
To overcome this issue, a full wireless solution is proposed which is the need of today’s
era.

Method:

This study presents a novel PID controller for a multi-loop pilot plant, utilizing a Bluetooth
Low Energy (BLE) based Internet of Thing (IoT) system for wireless, real-time data sensing
and control. The system gathers data through sensors and sends it to cloud storage via a BLE access
point, which is then monitored using a mobile app called BIP. In addition to monitoring, the BIP
app serves as a control interface, allowing PID parameters to be adjusted through a Quantum Firefly-
Particle Swarm Optimization (QFPSO) algorithm integrated with the ThingSpeak cloud. This
enables the control module to function in three distinct modes for the plant’s loops. Users can manually
configure PID parameters, as well as temperature and level set-points, while the system automatically
regulates the flow set-point based on real-time data. The BLE-based IoT system comprises
five modules using Arduino Nano 33 BLE: a Flow Sensor, a Temperature Sensor, a Level Sensor,
IoT communication, and an access point. These modules provide more accurate data than traditional
sensing systems.

Result:

Key benefits of the proposed system include wireless accessibility, user-friendliness, a simplified
design, ease of upgrades, and consistent control across multiple loops. The proposed system
can be easily adapted for various types of industrial control systems with minimal effort.

Conclusion:

Additionally, the developed wireless sensor node can replace wired sensor nodes in
any electronic system.