bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Plant-Derived Natural Compounds in Experimental Autoimmune Encephalomyelitis: A Review of the Treatment Potential and Development Strategy
Authors: Guo Yu-Xin , Zhang Yuan , Gao Yu-Han , Deng Si-Ying , Wang Li-Mei , Li Cui-Qin , Li Xing
Published: June 2021

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system that is mainly mediated by pathological T-cells. Experimental autoimmune encephalomyelitis (EAE) is a well-known animal model of MS that is used to study the underlying mechanism and offers a theoretical basis for developing a novel therapy for MS. Good therapeutic effects have been observed after the administration of natural compounds and their derivatives as treatments for EAE. However, there has been a severe lag in the research and development of drug mechanisms related to MS. This review examines natural products that have the potential to effectively treat MS. The relevant data were consulted in order to elucidate the regulated mechanisms acting upon EAE by the flavonoids, glycosides, and triterpenoids derived from natural products. In addition, novel technologies such as network pharmacology, molecular docking, and high-throughput screening have been gradually applied in natural product development. The information provided herein can help improve targeting and timeliness for determining the specific mechanisms involved in natural medicine treatment and lay a foundation for further study.
Salvage Therapy With Low-Dose Ruxolitinib Leads to a Significant Improvement in Bronchiolitis Obliterans Syndrome in Patients With cGVHD After Allogeneic Hematopoietic Stem Cell Transplantation
Authors: Zhao Yanmin , OuYang Guifang , Shi Jimin , Luo Yi , Tan Yamin , Yu Jian , Fu Huarui , Lai Xiaoyu , Liu Lizhen , Huang He
Published: June 2021

Bronchiolitis obliterans syndrome (BOS) is a life-threatening pulmonary manifestation of chronic graft versus host disease (cGVHD) post-allogeneic hematopoietic stem cell transplantation (HSCT), without clear standard of care. This study included 30 patients undergoing an allogeneic HSCT for a hematological malignancy and the outcomes with post-HSCT BOS treated with ruxolitinib as a salvage treatment were reviewed. After a median duration of ruxolitinib therapy of 9.25 (1.5–27) months, the best overall response (BOR) rate was 66.7%: three patients (10.0%) achieved complete remission, and 17 (56.7%) achieved partial remission. The median time from initiation of ruxolitinib to achieve the best responses was 3 months. Since initiating ruxolitinib, forced expiratory volume in 1 s of predicted (FEV1%pred) slightly increased after 3 and 6 months compared with measurements before ruxolitinib in responders. Only FEV1%pred mild decline before ruxolitinib with a ratio ≤15% was an independent predictor to achieve a response to ruxolitinib. Eleven patients (36.7%) had severe pulmonary infection of ≥3 grade. Following a median follow-up of 318 days after ruxolitinib, the 2-years incidence of nonrelapse mortality and 2-years overall survival rate after ruxolitinib among patients with BOS was 25.1 and 62.6%, respectively. Ruxolitinib is a promising treatment option to improve the prognosis of post-HSCT BOS.
Pharmacogenomics Implementation Training Improves Self-Efficacy and Competency to Drive Adoption in Clinical Practice
Authors: Adesta Fadhli , Mahendra Caroline , Junusmin Kathleen Irena , Rajah Arya Melissa Selva , Goh Sharon , Sani Levana , Chan Alexandre , Irwanto Astrid
Published: June 2021

Background: Administration of pharmacogenomics (PGx) testing in clinical practice has been suboptimal, presumably due to lack of PGx education. Here, we aim to evaluate the standpoint of PGx testing among a diverse group of healthcare professionals (HCPs) through conducting surveys before and after training.

Materials and Methods: Training modules were designed to cover three key learning objectives and deployed in five sections. A pre- and post-training survey questionnaire was used to evaluate participants’ self-assessments on employing PGx in clinical practice.

Results and Conclusion: Out of all enrollments, 102 survey responses were collected. Overall, respondents agree on the benefits of PGx testing, but have inadequate self-efficacy and competency in utilizing PGx data. Our results show that a 90 min long training significantly improves these, and could lead to greater anticipation of PGx adoption.
Functional Nanocarriers for Delivering Itraconazole Against Fungal Intracellular Infections
Authors: Mejía Susana P. , Sánchez Arturo , Vásquez Viviana , Orozco Jahir
Published: June 2021

Infectious diseases caused by intracellular microorganisms represent a significant challenge in medical care due to interactions among drugs during coinfections and the development of resistance in microorganisms, limiting existing therapies. This work reports on itraconazole (ITZ) encapsulated into functional polymeric nanoparticles for their targeted and controlled release into macrophages to fight intracellular infections. NPs are based on poly (lactic acid-co-glycolic acid) (PLGA) polymers of different compositions, molecular weights, and lactic acid–to–glycolic acid ratios. They were self-assembled using the high-energy nanoemulsion method and characterized by transmission electron microscopy, Fourier transform infrared spectroscopy (FT-IR), and differential scanning calorimetry. It was studied how the polymer-to-drug ratio, changes in the aqueous phase pH, and type and concentration of surfactant affected nanocarriers’ formation, drug-loading capacity, and encapsulation efficiency. Results showed that drug-loading capacity and encapsulation efficiency reached 6.7 and 80%, respectively, by lowering the pH to 5.0 and using a mixture of surfactants. Optimized formulation showed an initial immediate ITZ release, followed by a prolonged release phase that fitted better with a Fickian diffusion kinetic model and high stability at 4 and 37°C. NPs functionalized by using the adsorption and carbodiimide methods had different efficiencies, the carbodiimide approach being more efficient, stable, and reproducible. Furthermore, linking F4/80 and mannose to the NPs was demonstrated to increase J774A.1 macrophages’ uptake. Overall, in vitro assays showed the nanosystem’s efficacy to eliminate the Histoplasma capsulatum fungus and pave the way to design highly efficient nanocarriers for drug delivery against intracellular infections.
Kaempferol Alleviates Steatosis and Inflammation During Early Non-Alcoholic Steatohepatitis Associated With Liver X Receptor α-Lysophosphatidylcholine Acyltransferase 3 Signaling Pathway
Authors: Xiang Hongjiao , Shao Mingmei , Lu Yifei , Wang Junmin , Wu Tao , Ji Guang
Published: June 2021

Background: Kaempferol (KP) has a variety of biological effects such as anti-inflammatory, anti-oxidant, anti-aging and cardiovascular protection. Whether KP has a therapeutic effect on non-alcoholic steatohepatitis (NASH), and the detailed mechanism is currently unclear. This study aims to explore the mechanism of KP in the treatment of NASH through in vivo and in vitro experiments.

Methods: 1) In vivo experiment: In the C57BL/6 NASH mice model induced by high fat diet (HFD), KP was administered by gavage at a dose of 20 mg/kg/day. 2) In vitro experiment: Palmitic acid/Oleic acid (PA/OA, 0.375/0.75 mM) was used to intervene HepG2 and AML12 cells to establish a steatosis cell model. Three concentrations of KP, low (20 μmol/L), medium (40 μmol/L) and high (60 μmol/L) were used in vitro. The mRNA and protein expression of related molecules involved in LXRα-LPCAT3-ERS pathway were detected using RT-qPCR and Western blot.

Results: In the NASH mouse model, KP can significantly reduce the expression of LXRα, LPCAT3 and ERS-related factors PERK, eIF2α, ATF6, ATF4, XBP1, CHOP, IRE1α and GRP78. In the PA/OA-induced cell model, KP could decrease the content of triglyceride and lipid droplets, and also decrease the expression of LXR α, LPCAT3 and ERS related factors PERK, eIF2α, ATF6, ATF4, XBP1, CHOP, IRE1α and GRP78.

Conclusion: KP may decrease the expression level of LXRα and LPCAT3, thus improve ERS and reduce hepatic steatosis and inflammation.
TLR9 Signaling Protects Alcohol-Induced Hepatic Oxidative Stress but Worsens Liver Inflammation in Mice
Authors: Hao Liuyi , Zhong Wei , Sun Xinguo , Zhou Zhanxiang
Published: June 2021

Toll-Like Receptor 9 (TLR9) elicits cellular response to nucleic acids derived from pathogens or dead cells. Previous studies have shown that TLR9-driven response may lead to differential impact on the pathogenesis of liver diseases. This study aimed to determine how TLR9 may contribute to chronic alcohol exposure-induced liver pathogenesis. We observed that TLR9 KO mice were more susceptible to alcohol-induced liver injury, which was evidenced by higher serum ALT/AST levels and more lipid accumulation in alcohol-fed TLR9 KO mice than wild-type mice. Alcohol-induced oxidative stress and mitochondrial dysfunction were also exacerbated by TLR9 KO. We found that chronic alcohol exposure-induced hepatic CHOP and ATF6 activation were enhanced in TLR9 KO mice. By using primary hepatocytes and AML-12 cells, we confirmed that TLR9 activation by CpG ODN administration significantly ameliorated acetaldehyde-induced cell injury via suppressing ATF6-CHOP signaling. By using STAT3 knockdown AML12 cells, we showed that TLR9-mediated STAT3 activation inhibited ATF6-CHOP signaling cascade and thereby protecting against acetaldehyde-induced mitochondrial dysfunction and cell injury. Interestingly, we found that TLR9 KO mice ameliorate chronic alcohol exposure-induced CXCL1 induction and neutrophils infiltration in the liver. Furthermore, hepatocyte lack of STAT3 significantly ameliorated CpG ODN and LPS-increased CXCL1 levels in hepatocytes. Overall, our data demonstrate that TLR9 signaling in hepatocytes counteracts alcohol-induced hepatotoxicity but worsens proinflammatory response.
3D Direct Numerical Simulation on the Emergence and Development of Aeolian Sand Ripples
Authors: Huo Xinghui , Dun Hongchao , Huang Ning , Zhang Jie
Published: June 2021

A sand surface subjected to a continuous wind field exhibits a regular ripple surface. These aeolian sand ripples emerge and develop under the coupling effect between the wind field, bed surface topology, and sand particle transportation. Lots of theoretical and numerical models have been established to study the aeolian sand ripples since the last century, but none of them has the capability to directly reproduce the 3D long-term development of them. In this work, a novel numerical model with wind-blow sand and dynamic bedform is established. The emergence and long-term development of sand ripples can be obtained directly. The statistical results extracted from this model tally with those deduced from wind tunnel experiments and field observations. A simplified bed surface particle size description procedure is used in this model, which shows that the particle size distribution makes a very important contribution to sand ripples’ final steady state. This 3D bedform provides a more holistic view on the merging of small bumps before regular ripples’ formation. Analyzing the wind field results reveals an ignored development on the particle dynamic threshold during the bedform deformation.
Crossover of Failure Time Distributions in a Model of Time-Dependent Fracture
Authors: Alava Mikko J.
Published: June 2021

An important question in the theory of fracture is what kind of lifetime distributions may exist for materials under load. Here, this is studied in the context of a one-dimensional fracture model with local load sharing under a constant external load, “creep.” Simulations of the system with Weibull distributed initial lifetimes for the elements show that the limiting distribution follows from extreme statistics and takes the Gumbel form eventually, with longer and longer crossovers in the system size from a Weibull-like distribution, depending on the initial Weibull exponent.
Effect of Heat Stimulation on Circulating Irisin in Humans
Authors: Park Tae-Hwan , Lee Hye-Jin , Lee Jeong-Beom
Published: June 2021

High temperatures lead to oxidative stress. The aim of the study was to determine whether heat stimulation-induced hyperthermia can increase the level of circulating irisin. Twenty-one healthy female subjects (age, 26.3 ± 2.71 years; height, 162.1 ± 3.15 cm; weight, 54.2 ± 3.86 kg; and body surface area, 1.57 ± 0.11 m2) not taking contraceptives participated in this study. All experiments were performed individually for each participant when they were in the early proliferative menstrual phase. In an automated climate chamber (25 ± 0.5°C), the heat load was applied via half-body immersion into a hot water bath (42 ± 0.5°C). Five-minutes break was provided every after 5 min of immersion and the total passive heating time was 30 min. Tympanic temperature (Tty) and skin temperature (Ts) were measured. Mean body temperature (mTb) was calculated. Blood samples were collected before and immediately after immersion. Levels of irisin, cortisol, creatine kinase (CK), and lactate dehydrogenase (LDH) were analyzed. Tty, mTb and serum irisin levels increased after hot water immersion. The blood levels of cortisol, CK, and LDH were also elevated after hot water immersion. Heat stimulation might increase the levels of circulating irisin in humans in response to oxidative stress.
Deep Learning Framework for Real-Time Estimation of in-silico Thrombotic Risk Indices in the Left Atrial Appendage
Authors: Morales Ferez Xabier , Mill Jordi , Juhl Kristine Aavild , Acebes Cesar , Iriart Xavier , Legghe Benoit , Cochet Hubert , De Backer Ole , Paulsen Rasmus R. , Camara Oscar
Published: June 2021

Patient-specific computational fluid dynamics (CFD) simulations can provide invaluable insight into the interaction of left atrial appendage (LAA) morphology, hemodynamics, and the formation of thrombi in atrial fibrillation (AF) patients. Nonetheless, CFD solvers are notoriously time-consuming and computationally demanding, which has sparked an ever-growing body of literature aiming to develop surrogate models of fluid simulations based on neural networks. The present study aims at developing a deep learning (DL) framework capable of predicting the endothelial cell activation potential (ECAP), an in-silico index linked to the risk of thrombosis, typically derived from CFD simulations, solely from the patient-specific LAA morphology. To this end, a set of popular DL approaches were evaluated, including fully connected networks (FCN), convolutional neural networks (CNN), and geometric deep learning. While the latter directly operated over non-Euclidean domains, the FCN and CNN approaches required previous registration or 2D mapping of the input LAA mesh. First, the superior performance of the graph-based DL model was demonstrated in a dataset consisting of 256 synthetic and real LAA, where CFD simulations with simplified boundary conditions were run. Subsequently, the adaptability of the geometric DL model was further proven in a more realistic dataset of 114 cases, which included the complete patient-specific LA and CFD simulations with more complex boundary conditions. The resulting DL framework successfully predicted the overall distribution of the ECAP in both datasets, based solely on anatomical features, while reducing computational times by orders of magnitude compared to conventional CFD solvers.
Fire Blight Susceptibility in Lilium spp. Correlates to Sensitivity to Botrytis elliptica Secreted Cell Death Inducing Compounds
Authors: Malvestiti Michele C. , Immink Richard G. H. , Arens Paul , Quiroz Monnens Thomas , van Kan Jan A. L.
Published: June 2021

Fire blight represents a widespread disease in Lilium spp. and is caused by the necrotrophic Ascomycete Botrytis elliptica. There are >100 Lilium species that fall into distinct phylogenetic groups and these have been used to generate the contemporary commercial genotypes. It is known among lily breeders and growers that different groups of lilies differ in susceptibility to fire blight, but the genetic basis and mechanisms of susceptibility to fire blight are unresolved. The aim of this study was to quantify differences in fire blight susceptibility between plant genotypes and differences in virulence between fungal isolates. To this end we inoculated, in four biological replicates over 2 years, a set of 12 B. elliptica isolates on a panel of 18 lily genotypes representing seven Lilium hybrid groups. A wide spectrum of variation in symptom severity was observed in different isolate-genotype combinations. There was a good correlation between the lesion diameters on leaves and flowers of the Lilium genotypes, although the flowers generally showed faster expanding lesions. It was earlier postulated that B. elliptica pathogenicity on lily is conferred by secreted proteins that induce programmed cell death in lily cells. We selected two aggressive isolates and one mild isolate and collected culture filtrate (CF) samples to compare the cell death inducing activity of their secreted compounds in lily. After leaf infiltration of the CFs, variation was observed in cell death responses between the diverse lilies. The severity of cell death responses upon infiltration of the fungal CF observed among the diverse Lilium hybrid groups correlated well to their fire blight susceptibility. These results support the hypothesis that susceptibility to fire blight in lily is mediated by their sensitivity to B. elliptica effector proteins in a quantitative manner. Cell death-inducing proteins may provide an attractive tool to predict fire blight susceptibility in lily breeding programs.
Macro-Nutrient Stoichiometry of Glacier Algae From the Southwestern Margin of the Greenland Ice Sheet
Authors: Williamson Christopher J. , Turpin-Jelfs Thomas , Nicholes Miranda J. , Yallop Marian L. , Anesio Alexandre M. , Tranter Martyn
Published: June 2021

Glacier algae residing within the surface ice of glaciers and ice sheets play globally significant roles in biogeochemical cycling, albedo feedbacks, and melt of the world’s cryosphere. Here, we present an assessment of the macro-nutrient stoichiometry of glacier algal assemblages from the southwestern Greenland Ice Sheet (GrIS) margin, where widespread glacier algal blooms proliferate during summer melt seasons. Samples taken during the mid-2019 ablation season revealed overall lower cellular carbon (C), nitrogen (N), and phosphorus (P) content than predicted by standard microalgal cellular content:biovolume relationships, and elevated C:N and C:P ratios in all cases, with an overall estimated C:N:P of 1,997:73:1. We interpret lower cellular macro-nutrient content and elevated C:N and C:P ratios to reflect adaptation of glacier algal assemblages to their characteristic oligotrophic surface ice environment. Such lower macro-nutrient requirements would aid the proliferation of blooms across the nutrient poor cryosphere in a warming world. Up-scaling of our observations indicated the potential for glacier algal assemblages to accumulate ∼ 29 kg C km2 and ∼ 1.2 kg N km2 within our marginal surface ice location by the mid-ablation period (early August), confirming previous modeling estimates. While the long-term fate of glacier algal autochthonous production within surface ice remains unconstrained, data presented here provide insight into the possible quality of dissolved organic matter that may be released by assemblages into the surface ice environment.
Anxiety and Depression Symptom Level and Psychotherapy Use Among Music and Art Students Compared to the General Student Population
Authors: Vaag Jonas , Bjerkeset Ottar , Sivertsen Børge
Published: June 2021

Purpose: Previous epidemiological studies have shown higher levels of anxiety and depressive symptoms among professional musicians, compared to the general workforce. Similar findings have been observed for psychotherapy use among musicians. To date, large-scale investigations of prevalence rates among music and arts students are lacking.

Methods: Eight hundred and eighty students from music and arts institutions and faculties were derived from a national health student survey for higher education in Norway (the SHoT study). They were compared to a sample of the general student population (n = 48,729). We used logistic regression analysis, adjusting for age, sex, and semesters of study.

Results: Music and arts students reported higher rates of anxiety [OR 1.60 (1.38–1.85), Prevalence difference (PD) 9.6 (6.3–12.8)] and depression symptoms [OR 1.41 (1.22–1.62), PD 7.9 (4.5–11.2)] compared to the general student force. Similar patterns were observed for self-reported mental disorders [OR 1.71 (1.46–2.01), PD 8.1 (5.3–11.0)], as well as psychotherapy use [OR 1.91 (1.60–2.29), PD 7.4 (4.9–9.9)] in music and arts students.

Conclusions: Our findings are consistent with studies comparing musicians to the general workforce, and indicate that challenges also exist at student level, and not only after becoming a professional in the performing arts, which is important when planning health-related measures. These findings have the potential to inform on health promotion and services in the educational system.
Perceived Stress and Internet Addiction Among Chinese College Students: Mediating Effect of Procrastination and Moderating Effect of Flow
Authors: Gong Zhun , Wang Liyun , Wang Haijiao
Published: June 2021

Perceived stress, Internet addiction and procrastination are common issues among college students. Based on the Compensatory Internet Use (CIU) model and emotion regulation theory, this study aims to explore two possible mechanisms for the connection between perceived stress and Internet addiction: mediating effect of procrastination and moderating effect of flow experience on the Internet. Cross-sectional design and questionnaire survey were used in this study. Data were collected from 446 college students who voluntarily completed self-reporting of perceived stress, internet addiction, procrastination and flow. Potential relationship structure and moderation model between variables was calculated during the process. The results revealed that there were significant associations among perceived stress, Internet addiction, procrastination and flow. The results also showed that procrastination plays a mediating role between perceptual stress and Internet addiction, flow plays a moderating role between them. The results emphasized the importance of the intention behind college students’ overuse of the Internet. These results also provided a perspective of finding the possible causes of Internet addiction in college students, that is, individuals use the Internet to avoid stress and procrastinate, and the mobile experience on the Internet also affects the process.
Extra-Curricular Activities and Well-Being: Results From a Survey of Undergraduate University Students During COVID-19 Lockdown Restrictions
Authors: Finnerty Rachael , Marshall Sara A. , Imbault Constance , Trainor Laurel J.
Published: June 2021

Participation in extra-curricular activities has been found to associate with increased well-being. Here we investigated in a survey (n = 786) what activities university students at a Canadian university engaged in during the stressful COVID-19 pandemic lockdown in April, 2020, which coincided with a novel online exam period, and how these activities related to perceived well-being, anxiety (STAI-S), social aspects of activities, and personality. Sixty-five percentage of students scored in the high anxiety category of the STAI-S, an alarming statistic given that only 24% had reached out for professional supports. This is consistent with reports that current supports on university campuses are inadequate. Listening to music (92%) and watching movies/series (92%) were engaged in most frequently, followed by socializing virtually (89%) and engaging in social media (85%). The activities students rated as most helpful to their well-being were somewhat different, with outdoor exercise rated highest, followed by socializing virtually and listening to music. While all activities were rated as beneficial, those with a social component tended to have high ratings, consistent with students attempting to replace lost social interactions. Linear regression models found few associations between STAI-S scores and other measures, likely because of large individual differences and lack of a pre-pandemic baseline needed to assess changes in anxiety. The importance of individual differences was evident in that those higher in conscientiousness or extraversion or emotional stability were more likely to engage in exercise, while those higher in openness to experience were more likely to engage in journaling, playing a musical instrument, or singing, with a trend for higher engagement in song writing. Individual differences were also evident in that equal numbers of students gave positive and negative comments related to their well-being during the pandemic. The individual differences uncovered here suggest that having a variety of proactive interventions would likely reach more students. Indeed, 52% indicated an interest in online group music therapy, 48% in art therapy and 40% in verbal therapy, despite music and art therapies being virtually non-existent on campuses. In sum, the findings highlight the importance of choice in extra-curricular activities and therapies that support well-being.
Musical and Bodily Predictors of Mental Effort in String Quartet Music: An Ecological Pupillometry Study of Performers and Listeners
Authors: Bishop Laura , Jensenius Alexander Refsum , Laeng Bruno
Published: June 2021

Music performance can be cognitively and physically demanding. These demands vary across the course of a performance as the content of the music changes. More demanding passages require performers to focus their attention more intensity, or expend greater “mental effort.” To date, it remains unclear what effect different cognitive-motor demands have on performers' mental effort. It is likewise unclear how fluctuations in mental effort compare between performers and perceivers of the same music. We used pupillometry to examine the effects of different cognitive-motor demands on the mental effort used by performers and perceivers of classical string quartet music. We collected pupillometry, motion capture, and audio-video recordings of a string quartet as they performed a rehearsal and concert (for live audience) in our lab. We then collected pupillometry data from a remote sample of musically-trained listeners, who heard the audio recordings (without video) that we captured during the concert. We used a modelling approach to assess the effects of performers' bodily effort (head and arm motion; sound level; performers' ratings of technical difficulty), musical complexity (performers' ratings of harmonic complexity; a score-based measure of harmonic tension), and expressive difficulty (performers' ratings of expressive difficulty) on performers' and listeners' pupil diameters. Our results show stimulating effects of bodily effort and expressive difficulty on performers' pupil diameters, and stimulating effects of expressive difficulty on listeners' pupil diameters. We also observed negative effects of musical complexity on both performers and listeners, and negative effects of performers' bodily effort on listeners, which we suggest may reflect the complex relationships that these features share with other aspects of musical structure. Looking across the concert, we found that both of the quartet violinists (who exchanged places halfway through the concert) showed more dilated pupils during their turns as 1st violinist than when playing as 2nd violinist, suggesting that they experienced greater arousal when “leading” the quartet in the 1st violin role. This study shows how eye tracking and motion capture technologies can be used in combination in an ecological setting to investigate cognitive processing in music performance.
Perceptions of the Post First-Lockdown Era in the Current Covid-19 Pandemic: Quantitative and Qualitative Survey of the French Population
Authors: Fouques Damien , Castro Dana , Mouret Marion , Julien-Sweerts Sabrina , Romo Lucia
Published: June 2021

Introduction:A pandemic with the severity of COVID-19 affects people's lives physically, as well as their daily routines, views of the world, and emotional balance. Lockdown is often an unpleasant experience due to a separation from loved ones, loss of freedom, and uncertainty over the disease status. To adjust, individuals and groups have had to adapt their perceptions of the event to the current scenario. This study aims to describe the perceptions of confined people on the changes occurring in their lives in the aftermath of the COVID-19 lockdown.

Methods:A total of 1,534 individuals (26.6% men; 73.4% women; mean age 41.6) responded to the questionnaire comprising 19 closed and five open-ended questions about the changes they anticipated in their lives in the immediate post-confinement era.

Results:Two definite groups appeared in the results: those who lived the confinement pleasantly, and those for which it was painful. They differ according to their confinement conditions and perceived degree of exposure to the virus. There seems to be a link for those who had a pleasant experience to a lower perceived exposure to the virus and less burdensome confinement conditions (young children, surface area, etc.). Lockdown conditions seem to influence the respondents' perceptions: a pleasant experience is associated with a vision of the society's evolution at large, and the care about its economic and professional progress; a painful one is associated more with focusing on the immediate needs of social support and personal well-being.

Discussion:Emotional experience during lockdown impacts the perception of its aftermath, with hope and anxiety becoming two ways of coping with uncertainty.
Positive Participatory Organizational Interventions: A Multilevel Approach for Creating Healthy Workplaces
Authors: Nielsen Karina , Christensen Marit
Published: June 2021

In the following perspective paper, we argue for the importance of conducting research on positive participatory organizational interventions. We propose that these types of interventions are important because they not only focus on eliminating or reducing adverse job demands but focus also on developing job resources. To achieve the best effects, actions should be taken to address demands and resources at the individual, group, leader and organizational levels. We furthermore suggest that the participatory intervention process itself may also build resources at these four levels.
Optimized Projection and Fisher Discriminative Dictionary Learning for EEG Emotion Recognition
Authors: Gu Xiaoqing , Fan Yiqing , Zhou Jie , Zhu Jiaqun
Published: June 2021

Electroencephalogram (EEG)-based emotion recognition (ER) has drawn increasing attention in the brain–computer interface (BCI) due to its great potentials in human–machine interaction applications. According to the characteristics of rhythms, EEG signals usually can be divided into several different frequency bands. Most existing methods concatenate multiple frequency band features together and treat them as a single feature vector. However, it is often difficult to utilize band-specific information in this way. In this study, an optimized projection and Fisher discriminative dictionary learning (OPFDDL) model is proposed to efficiently exploit the specific discriminative information of each frequency band. Using subspace projection technology, EEG signals of all frequency bands are projected into a subspace. The shared dictionary is learned in the projection subspace such that the specific discriminative information of each frequency band can be utilized efficiently, and simultaneously, the shared discriminative information among multiple bands can be preserved. In particular, the Fisher discrimination criterion is imposed on the atoms to minimize within-class sparse reconstruction error and maximize between-class sparse reconstruction error. Then, an alternating optimization algorithm is developed to obtain the optimal solution for the projection matrix and the dictionary. Experimental results on two EEG-based ER datasets show that this model can achieve remarkable results and demonstrate its effectiveness.
Psychomotor Agitation Non-responsive to Treatment: A Case Report of Phenibut Withdrawal Syndrome
Authors: Esposito Cecilia Maria , Mandolini Gian Mario , Delvecchio Giuseppe , Fiorentini Alessio , Brambilla Paolo
Published: June 2021

Background and Objectives: Phenibut (4-amino-3-phenyl-butyric acid), acting as a GABA-B receptor agonist, has a beneficial effect on anxiety. Although its medical use is not approved in western countries, it can be easily obtained worldwide via the Internet, so it spread as a substance of abuse. In recent years, some case reports have, therefore, highlighted episodes of acute toxicity or withdrawal, but it is still a largely unknown phenomenon.

Methods: In this case report, a 50-year-old woman was admitted to the emergency room with psychomotor agitation, psychotic symptoms, and insomnia, and was non-responsive to treatment. She was hospitalized at the psychiatry ward for 25 days and gave her consent for the publication of the present case report.

Results: The suspicion of phenibut withdrawal allowed to establish the appropriate management, leading to the restitutio ad integrum of the psychopathological case.

Conclusions: In the face of an incoercible psychomotor agitation case, the knowledge of the so-called novel psychoactive substances allows for more appropriate clinical management of intoxication and withdrawal syndromes. This is a scientifically significant report as it provides therapeutic and outcome data concerning a syndrome that is still quite unfamiliar.
(Re)production of Inequalities in Retirement Practices and Meanings Assigned to the Term ‘Retiree’ in the Post-Communist Context
Authors: Urbaniak Anna
Published: June 2021

In the institutionalized life course transition from work to retirement is the transition that culturally defines the beginning of later life. However, there is no universal way of experiencing retirement or understanding retirees’ social roles. Especially in the context of the post-communist, liquid modern reality in Poland. The social role of the retiree, defined as a set of rules and expectations generated for individuals occupying particular positions in the social structure, is constructed at the intersection of what is culturally defined and individually negotiated. Therefore, the way in which individuals (re)define term “retiree” and “do retirement” reflects not only inequalities in individual resources and attitudes, but also in social structure in a given place and at a given time. In this contribution, I draw upon data from 68 qualitative interviews with retirees from Poland to analyze retirement practices and meanings assigned to the term “retiree.” Applying practice theory, I explore the inequalities they (re)produce, mirror and reinforce at the same time. Results show that there are four broad types of retirement practices: caregiving, working, exploring and disengaging. During analysis of meanings assigned by participants to the term “retiree,” two definitions emerged: one of a “new wave retiree” and the other of a “stagnant retiree.” Results suggest that in the post-communist context, retirement practices and meanings assigned to the term “retiree” are in the ongoing process of (re)negotiation and are influenced on the one hand by the activation demands resulting from discourses of active and productive aging, and on the other by habitus and imaginaries of retirement formed in the bygone communist era. Retirement practices and definitions of the term “retiree” that emerged from the data reflect structural and individual inequalities, highlighting intersection of gender, age and socioeconomic status in the (re)production of inequalities in retirement transition in the post-communist context.
Self-Adaptive Path Tracking Control for Mobile Robots under Slippage Conditions Based on an RBF Neural Network
Authors: Kang Yiting , Xue Biao , Zeng Riya
Published: June 2021

Wheeled mobile robots are widely implemented in the field environment where slipping and skidding may often occur. This paper presents a self-adaptive path tracking control framework based on a radial basis function (RBF) neural network to overcome slippage disturbances. Both kinematic and dynamic models of a wheeled robot with skid-steer characteristics are established with position, orientation, and equivalent tracking error definitions. A dual-loop control framework is proposed, and kinematic and dynamic models are integrated in the inner and outer loops, respectively. An RBF neutral network is employed for yaw rate control to realize adaptability to longitudinal slippage. Simulations employing the proposed control framework are performed to track snaking and a DLC reference path with slip ratio variations. The results suggest that the proposed control framework yields much lower position and orientation errors compared with those of a PID and a single neuron network (SNN) controller. It also exhibits prior anti-disturbance performance and adaptability to longitudinal slippage. The proposed control framework could thus be employed for autonomous mobile robots working on complex terrain.
A New Breeding Strategy towards Introgression and Characterization of Stay-Green QTL for Drought Tolerance in Sorghum
Authors: Kamal Nasrein Mohamed , Gorafi Yasir Serag Alnor , Abdeltwab Hanan , Abdalla Ishtiag , Tsujimoto Hisashi , Ghanim Abdelbagi Mukhtar Ali
Published: June 2021

Several marker-assisted selection (MAS) or backcrossing (MAB) approaches exist for polygenic trait improvement. However, the implementation of MAB remains a challenge in many breeding programs, especially in the public sector. In MAB introgression programs, which usually do not include phenotypic selection, undesired donor traits may unexpectedly turn up regardless of how expensive and theoretically powerful a backcross scheme may be. Therefore, combining genotyping and phenotyping during selection will improve understanding of QTL interactions with the environment, especially for minor alleles that maximize the phenotypic expression of the traits. Here, we describe the introgression of stay-green QTL (Stg1–Stg4) from B35 into two sorghum backgrounds through an MAB that combines genotypic and phenotypic (C-MAB) selection during early backcross cycles. The background selection step is excluded. Since it is necessary to decrease further the cost associated with molecular marker assays, the costs of C-MAB were estimated. Lines with stay-green trait and good performance were identified at an early backcross generation, backcross two (BC2). Developed BC2F4 lines were evaluated under irrigated and drought as well as three rainfed environments varied in drought timing and severity. Under drought conditions, the mean grain yield of the most C-MAB-introgression lines was consistently higher than that of the recurrent parents. This study is one of the real applications of the successful use of C-MAB for the development of drought-tolerant sorghum lines for drought-prone areas.
Review on Control Methods against Plant Parasitic Nematodes Applied in Southern Member States (C Zone) of the European Union
Authors: Sasanelli Nicola , Konrat Alena , Migunova Varvara , Toderas Ion , Iurcu-Straistaru Elena , Rusu Stefan , Bivol Alexei , Andoni Cristina , Veronico Pasqua
Published: June 2021

The European legislative on the use of different control strategies against plant-parasitic nematodes, with particular reference to pesticides, is constantly evolving, sometimes causing confusion in the sector operators. This article highlights the nematode control management allowed in the C Zone of the European Union, which includes the use of chemical nematicides (both fumigant and non-fumigant), agronomic control strategies (crop rotations, biofumigation, cover crops, soil amendments), the physical method of soil solarization, the application of biopesticides (fungi, bacteria and their derivatives) and plant-derived formulations. The authors analyze the use of these strategies and substances in organic agriculture as well as in Integrated Pest Management (IPM) programs.
Correspondence between SOC1 Genotypes and Time of Endodormancy Break in Peach (Prunus persica L. Batsch) Cultivars
Authors: Halász Júlia , Hegedűs Attila , Karsai Ildikó , Tósaki Ágnes , Szalay László
Published: June 2021

Knowledge of dormancy traits are important in peach breeding. Traditional method selection of seedlings takes a long time because of the juvenile period of plants; therefore, novel application of marker assisted selection methods are needed to accelerate this work. The aims of this study were to test the extent of variability in the PpSOC1 gene among 16 peach cultivars and to establish whether the variability of SOC1 can be used as a functional marker for the timing of endodormancy break based on a 14-year phenology evaluation covering nine consecutive phenology phases, from string stage to ripening. Based on an SSR motif of SOC1, three allele categories were detected: one peach cultivar was heterozygous (203/209), while five of the 15 homozygous cultivars carried a 203 bp allele and the remainder were characterized with 218 bp. There were significant correlations between the PpSOC1 alleles and the various phenology phases, the strongest one being observed at the string stage, marking the end of endodormancy. At this stage, PpSOC1 explained 82.6% of the phenotypic variance; cultivars with the 203 allele reached the string stage 11.7 days earlier than those with 218 bp allele. This finding makes the PpSOC1 screening a valuable method in breeding.
Estimating Pesticide Inputs and Yield Outputs of Conventional and Organic Agricultural Systems in Europe under Climate Change
Authors: Rasche Livia
Published: June 2021

Simulating organic agriculture is a considerable challenge. One reason is that few models are capable of simulating crop-pest interactions and the yield losses they cause. Here, a recently developed process-based crop-pest model (Pest-EPIC) was used to simulate conventional and organic agriculture in the European Union for the years 1995–2100. Yields and pesticide application rates were calibrated against FAOSTAT and Eurostat data. Results indicate that current pesticide application rates may be sufficient to control pests and diseases even at the end of the century. The range of simulated yield differences under organic and conventional agriculture under current conditions (e.g., wheat 21–55% (mean 34%) lower yields; potatoes 20–99% (mean 56%) lower yields) closely matched recorded values. Under climate change, the gap between yields under conventional and organic management will remain constant for some crops (e.g., at 3 t/ha for potatoes), but others—susceptible to a larger number of pests and diseases—may experience a widening of the yield gap (e.g., increase of yield difference from 0.8 to 1.6 t/ha for wheat). The presented results-dataset may in future be a valuable resource for integrated assessments of agricultural land use and policy planning, but the inherent uncertainty is still very high.
Effect of Shungite Application on the Temperature Sensitivity of Allium cepa Respiration under Two Soil Water Regimes
Authors: Ikkonen Elena , Chazhengina Svetlana , Bakhmet Olga , Sidorova Valeria
Published: June 2021

For agricultural soils with low natural fertility, the possibility of using rock powders as an alternative source of nutrients and/or improver of soil physical parameters is under discussion and study. Shungite rocks, carbon-bearing volcanic sedimentary rock, are characterized by a high content of carbon and nutrients. This study aimed to evaluate whether shungite application to Umbric Podzols may affect leaf and root mitochondrial respiratory pathways, and the leaf response to temperature change. A pot culture experiment was conducted with Allium cepa L. seedlings, using soil shungite concentrations of 0, 5, 10, and 20 g kg−1 and two soil water regimes: well-watered (WW) and drying-wetting (DW) cycles. Soil water deficit increased total respiration (Vt) of onion leaves, but not roots, under low (13 °C) and high (33 °C) measurement temperature. Shungite application affected leaf Vt only at 13 °C: it increased the Vt rate under WW and decreased one under DW. An increase in the measurement temperature to 33 °C enhanced the sensitivity of leaf respiration to the inhibitor of the alternative respiratory pathway (salicylhydroxamic acid, SHAM). Shungite application increased the contribution of SHAM-sensitive pathway to the leaf Vt rate under WW, but not under the DW regime, regardless of the leaf temperature. In contrast to the SHAM-resistant pathway, the temperature sensitivity of the SHAM-sensitive rate decreased following the decrease in soil water availability. Shungite application increased the temperature sensitivity of both SHAM-sensitive and SHAM-resistant pathways under DW, and significantly decreased these parameters under WW. In summary, the decrease in temperature sensitivity of alternative SHAM-sensitive respiratory pathway with a decrease of soil water availability or shungite-related decrease of both SHAM-sensitive and SHAM-resistant leaf respiration may play an important role in enhancing the resistance of plant respiration to stress temperature.
Considering Human Variability in the Design of Safe Interaction with Agricultural Machinery: The Case of Foldable Roll-Over Protective Structure (FROPS) Manual Handling
Authors: Micheletti Cremasco Margherita , Vigoroso Lucia , Caffaro Federica , Paletto Giuseppe , Cavallo Eugenio
Published: June 2021

The foldable roll-over protective structure (FROPS) protects the operators against fatal injuries in tractor roll-over accidents. However, a rear-mounted FROPS is often folded down or removed. In the present study, the accessible zones and grasping areas in a rear-mounted FROPS were redesigned and adapted to the 5th, 50th, and 95th European human anthropometric percentiles to enhance its correct and comfortable use. Then, a rod was proposed as a design solution to make the roll-bar grasping areas fall within the new accessible zones. The rod prototype increased roll-bar reachability and facilitated the raising handling, especially for shorter users. The present study results and the accessible zones redesigned, taking into account the human percentiles, will be helpful in rethinking reachability issues in manual handling of machinery components, to support the correct behaviours, and make human-machine interaction more comfortable and safer for all.