bluuebunny/crossref_metadata_2025 huggingface dataset:
Tree-Ring Growth of Larch (Larix decidua Mill.) in the Polish Sudetes—The Influence of Altitude and Site-Related Factors on the Climate–Growth Relationship
Authors: Danek Małgorzata , Chuchro Monika , Walanus Adam
Published: October 2018

In this paper, the first study of a regional character on the influence of climatic factors on the tree-ring growth of European larch (Larix decidua Mill.) growing in the Polish Sudetes is presented. The obtained results indicate the relatively high diversity of the climatic signal observed in the tree rings of larches growing in the Sudetes. The most significant differentiating factor is altitude. The results suggest that the possible influence of local conditions (e.g., summit proximity, soil and bedrock characteristics, and exposure to strong winds) could also be of importance. A positive relationship between tree-ring growth and May temperatures was noted throughout the area; this indicates the principal importance of thermal conditions during the initial stage of cambial activity and tree-ring formation in larches from the Sudetes. The negative effect of the temperatures in the previous summer upon the tree-ring growth of larch in the subsequent year was also observed. The studies also indicate the negative influence of the water stress in summer (particularly in July of the previous year) upon the growth of trees. The negative relationship between tree-ring growth and the previous November temperature could be explained by the need for a late-autumn cooling, which affects the development of assimilation apparatus in spring of the subsequent year, which indirectly affects the tree-ring growth in the same year.
Resource Allocation, Pit Quality, and Early Survival of Seedlings Following Two Motor-Manual Pit-Drilling Options
Authors: Boja Nicuşor , Boja Florinel , Teusdea Alin , Vidrean Dan , Marcu Marina Viorela , Iordache Eugen , Duţă Cristian Ionuţ , Borz Stelian Alexandru
Published: October 2018

Afforestation and reforestation operations constitute an important part of the forest management, being crucial for the sustainability of forests. In such operations, there are three options to prepare the planting holes: manual, partly mechanized, and fully mechanized. Given the high cost of mechanized planting and the ergonomic issues of manual planting, one option which is worth exploring is using of augers, because they have the potential to mitigate and/or eliminate intense physical effort and aspects of some of the ergonomic problems. This study examines the early survival of seedlings following the use of augers to prepare the planting pits. Working time, fuel consumption and physical quality of the pits were evaluated on nine sites for two drill types differentiated by their diameter (150 vs. 200 mm). Time consumption was systematically higher when using the larger drill, while fuel consumption was not found to be statistically different. The larger drill systematically produced pits characterized by less physical quality in terms of resistance to penetration and shear strength, but the early survival of seedlings was higher when using this drill size. Survival probability modeled by means of logistic regression showed that pit size was among the factors that may affect the early survival of seedlings. The study concludes that the larger drill would be more appropriate to plant seedlings, but further studies should be arranged to see if long-term survival would be affected in this case.
Modeling of Carbon Dioxide Leakage from Storage Aquifers
Authors: Heidari Parvaneh , Hassanzadeh Hassan
Published: October 2018

Long-term geological storage of CO2 in deep saline aquifers offers the possibility of sustaining access to fossil fuels while reducing emissions. However, prior to implementation, associated risks of CO2 leakage need to be carefully addressed to ensure safety of storage. CO2 storage takes place by several trapping mechanisms that are active on different time scales. The injected CO2 may be trapped under an impermeable rock due to structural trapping. Over time, the contribution of capillary, solubility, and mineral trapping mechanisms come into play. Leaky faults and fractures provide pathways for CO2 to migrate upward toward shallower depths and reduce the effectiveness of storage. Therefore, understanding the transport processes and the impact of various forces such as viscous, capillary and gravity is necessary. In this study, a mechanistic model is developed to investigate the influence of the driving forces on CO2 migration through a water saturated leakage pathway. The developed numerical model is used to determine leakage characteristics for different rock formations from a potential CO2 storage site in central Alberta, Canada. The model allows for preliminary analysis of CO2 leakage and finds applications in screening and site selection for geological storage of CO2 in deep saline aquifers.
Fatigue-Induced Cole Electrical Impedance Model Changes of Biceps Tissue Bioimpedance
Authors: Freeborn Todd J. , Fu Bo
Published: October 2018

Bioimpedance, or the electrical impedance of biological tissues, describes the passive electrical properties of these materials. To simplify bioimpedance datasets, fractional-order equivalent circuit presentations are often used, with the Cole-impedance model being one of the most widely used fractional-order circuits for this purpose. In this work, bioimpedance measurements from 10 kHz to 100 kHz were collected from participants biceps tissues immediately prior and immediately post completion of a fatiguing exercise protocol. The Cole-impedance parameters that best fit these datasets were determined using numerical optimization procedures, with relative errors of within approximately ± 0.5 % and ± 2 % for the simulated resistance and reactance compared to the experimental data. Comparison between the pre and post fatigue Cole-impedance parameters shows that the R ∞ , R 1 , and f p components exhibited statistically significant mean differences as a result of the fatigue induced changes in the study participants.
Ex Post Nash Equilibrium in Linear Bayesian Games for Decision Making in Multi-Environments
Authors: Edalat Abbas , Hossein Ghorban Samira , Ghoroghi Ali
Published: October 2018

We show that a Bayesian game where the type space of each agent is a bounded set of m-dimensional vectors with non-negative components and the utility of each agent depends linearly on its own type only is equivalent to a simultaneous competition in m basic games which is called a uniform multigame. The type space of each agent can be normalised to be given by the ( m − 1 ) -dimensional simplex. This class of m-dimensional Bayesian games, via their equivalence with uniform multigames, can model decision making in multi-environments in a variety of circumstances, including decision making in multi-markets and decision making when there are both material and social utilities for agents as in the Prisoner’s Dilemma and the Trust Game. We show that, if a uniform multigame in which the action set of each agent consists of one Nash equilibrium inducing action per basic game has a pure ex post Nash equilibrium on the boundary of its type profile space, then it has a pure ex post Nash equilibrium on the whole type profile space. We then develop an algorithm, linear in the number of types of the agents in such a multigame, which tests if a pure ex post Nash equilibrium on the vertices of the type profile space can be extended to a pure ex post Nash equilibrium on the boundary of its type profile space in which case we obtain a pure ex post Nash equilibrium for the multigame.
The Power of Low Frequencies: Faraday Tomography in the Sub-GHz Regime
Authors: Van Eck Cameron L.
Published: October 2018

Faraday tomography, the study of the distribution of extended polarized emission by strength of Faraday rotation, is a powerful tool for studying magnetic fields in the interstellar medium of our Galaxy and nearby galaxies. The strong frequency dependence of Faraday rotation results in very different observational strengths and limitations for different frequency regimes. I discuss the role these effects take in Faraday tomography below 1 GHz, emphasizing the 100–200 MHz band observed by the Low Frequency Array and the Murchison Widefield Array. With that theoretical context, I review recent Faraday tomography results in this frequency regime, and discuss expectations for future observations.
Karyotype Characterization of Nine Periwinkle Species (Gastropoda, Littorinidae)
Authors: García-Souto Daniel , Alonso-Rubido Sandra , Costa Diana , Eirín-López José M. , Rolán-Álvarez Emilio , Faria Rui , Galindo Juan , Pasantes Juan J.
Published: October 2018

Periwinkles of the family Littorinidae (Children, 1834) are common members of seashore littoral communities worldwide. Although the family is composed of more than 200 species belonging to 18 genera, chromosome numbers have been described in only eleven of them. A molecular cytogenetic analysis of nine periwinkle species, the rough periwinkles Littorina arcana, L. saxatilis, and L. compressa, the flat periwinkles L. obtusata and L. fabalis, the common periwinkle L. littorea, the mangrove periwinkle Littoraria angulifera, the beaded periwinkle Cenchritis muricatus, and the small periwinkle Melarhaphe neritoides was performed. All species showed diploid chromosome numbers of 2n = 34, and karyotypes were mostly composed of metacentric and submetacentric chromosome pairs. None of the periwinkle species showed chromosomal differences between male and female specimens. The chromosomal mapping of major and minor rDNA and H3 histone gene clusters by fluorescent in situ hybridization demonstrated that the patterns of distribution of these DNA sequences were conserved among closely related species and differed among less related ones. All signals occupied separated loci on different chromosome pairs without any evidence of co-localization in any of the species.
Genetic Diversity, Population Structure, and Linkage Disequilibrium in a Spanish Common Bean Diversity Panel Revealed through Genotyping-by-Sequencing
Authors: Campa Ana , Murube Ester , Ferreira Juan José
Published: October 2018

A common bean (Phaseolus vulgaris) diversity panel of 308 lines was established from local Spanish germplasm, as well as old and elite cultivars mainly used for snap consumption. Most of the landraces included derived from the Spanish common bean core collection, so this panel can be considered to be representative of the Spanish diversity for this species. The panel was characterized by 3099 single-nucleotide polymorphism markers obtained through genotyping-by-sequencing, which revealed a wide genetic diversity and a low level of redundant material within the panel. Structure, cluster, and principal component analyses revealed the presence of two main subpopulations corresponding to the two main gene pools identified in common bean, the Andean and Mesoamerican pools, although most lines (70%) were associated with the Andean gene pool. Lines showing recombination between the two gene pools were also observed, most of them showing useful for snap bean consumption, which suggests that both gene pools were probably used in the breeding of snap bean cultivars. The usefulness of this panel for genome-wide association studies was tested by conducting association mapping for determinacy. Significant marker–trait associations were found on chromosome Pv01, involving the gene Phvul.001G189200, which was identified as a candidate gene for determinacy in the common bean.
Is the Kornati National Park Still an Acceptable Reference Area for Environmental Studies?
Authors: Ilenič Anja , Lojen Sonja , Župan Ivan , Šarić Tomislav , Šikić Zoran , Vrhovnik Petra , Dolenec Matej
Published: October 2018

The Kornati National Park (Croatia) is considered an environment with minimal anthropogenic input. The purpose of this study was to determine the isotopic characteristics of the sediment and muscle tissues of the banded dye-murex Hexaplex trunculus. We selected locations in the park according to their estimated risk of anthropogenic pollution (large, lower, and minimal). Isotopic analyses of the sedimentary organic carbon (δ13Corg values) showed that the sedimentary organic matter in locations with P. oceanica meadows (Piškera, Vrulje) was enriched in 13C compared to that of locations with the influx of terrestrial organic matter. The δ13C and δ15N values of the muscle tissues of H. trunuclus were the highest in the two locations with the highest possible anthropogenic impact (−14.47‰ and −15.66‰ for δ13Corg, +8.87‰ and +10.4‰ for δ15N). The high δ values may indicate the presence of the pigment indirubin (C16H10O2N2) and other derivatives that cause the purple coloration but are also elevated because of the discharge of untreated sewage from a nearby marina and village.
Exploring Risk Factors Affecting the Mental Health of Refugee Women Living with HIV
Authors: Vitale Agata , Ryde Judy
Published: October 2018

Little is known about how the intersection of being a forced migrant and living with HIV can contribute to the development or exacerbation of pre-existing mental conditions. This study is set in this context and it aims to explore specific risk factors affecting the mental health of refugee women living with HIV. A total of eight refugee women living with HIV took part in the study; they were individually interviewed, and their transcripts were thematically analyzed. The overall findings indicated that participants’ mental health was impaired by multiple stressors associated with their conditions, such as racial discrimination, HIV-related stigma, including from health professionals, loneliness, and resettlement adversities. These all represent threats to public health, as they discourage individuals from engaging with adequate health/mental health services. Despite their situation, participants had not received psychological interventions and their healthcare was reduced to managing the physical symptoms of HIV. Participants indicated their need to take part in group interventions that could promote their mental health and social recovery. These findings are relevant to raising awareness about the specific risk factors affecting refugee women living with HIV and to provide evidence for public health interventions based on this specific population’s need.
Adsorption of Cadmium on Degraded Soils Amended with Maize-Stalk-Derived Biochar
Authors: Wu Caixia , Li Yungui , Chen Mengjun , Luo Xiang , Chen Yuwei , Belzile Nelson , Huang Sheng
Published: October 2018

Biochar has been extensively proven to distinctively enhance the sorption capacity of both heavy metal and organic pollutants and reduce the related environmental risks. Soil pollution and degradation widely coexist, and the effect of biochar addition on adsorption behavior by degraded soils is not well understood. Four degraded soils with different degrees of degradation were amended with maize-stalk-derived biochar to investigate the adsorption of cadmium using batch methods. The maximum adsorption capacity (Qm) of degraded soil remarkably decreased in comparison with undegraded soil (5361 mg·kg−1→170 mg·kg−1), and the Qm of biochar increased with increasing pyrolysis temperature (22987 mg·kg−1→49016 mg·kg−1) which was much higher than that of soil. The addition of biochar can effectively improve the cadmium adsorption capacity of degraded soil (36–328%). The improving effect is stronger when increasing either the degradation level or the amount of added biochar, or the pyrolysis temperature of biochar. Contrary to the general soil–biochar system, adsorption of Cd was not enhanced but slightly suppressed (7.1–36.6%) when biochar was incorporated with degraded soils, and the adsorptivity attenuation degree was found to be negatively linear with SOM content in the degraded soil–biochar system. The results of the present study suggest that more attention on the adsorption inhibition and acceleration effect difference between the soil–biochar system and the degraded soil–biochar system is needed.
Prevalence of Anaemia and Iron Deficiency among Primary Schoolchildren in Malaysia
Authors: Nik Shanita Safii , Siti Hanisa Awal , Noor Afifah Ab Rahim , Lee Shoo Thien , Chong Kar Hau , George Penny , Norazida Abu Bakar , Budin Siti Balkis , Khouw Ilse , Norimah A Karim , Poh Bee Koon
Published: October 2018

The present study aimed to report the prevalence of anaemia and iron deficiency (ID) and to explore the associations among socio-demographic characteristics, nutritional status and inflammation status in the occurrence of anaemia and ID in a nationally representative sample of Malaysian primary schoolchildren. Using data from the South East Asian Nutrition Surveys (SEANUTS), 544 Malaysian children aged 7 to 12 years were included in this secondary analysis. Blood samples were drawn for haemoglobin and serum ferritin analysis while C-reactive protein (CRP) and α-1-acid glycoprotein (AGP) were measured to detect inflammation. Prevalence of anaemia and ID were 4.0% and 5.2%, respectively. There were significantly more anaemic indigenous bumiputra children (9.9%) than Chinese children (0.6%). Correction for inflammation did not change the prevalence of ID. More overweight/obese children than thin/normal weight children were found to have elevated acute phase protein (APP). Children with elevated inflammatory markers had significantly higher ferritin level than children without inflammation. Periodic health assessments of anaemia and ID at the population level to monitor and clarify the epidemiology of health problems are required to inform public health policies and strategies.
Removal of Cadmium and Lead from Contaminated Soils Using Sophorolipids from Fermentation Culture of Starmerella bombicola CGMCC 1576 Fermentation
Authors: Qi Xiaoyu , Xu Xiaoming , Zhong Chuanqing , Jiang Tianyi , Wei Wei , Song Xin
Published: October 2018

Soil contaminated with Cd and Pb has caused sharp decrease of cultivatable soil and has been attracting increasing attention. Biosurfactants are efficient in solving the problem. However, little information is available about the influence of sophorolipids (SLs) on the remediation of Cd- or Pb-contaminated soil. The sophorolipids produced by Starmerella bombicola CGMCC 1576 were used to study the effects of Cd and Pb removal in batch soil washing from artificially contaminated soil. The removal efficiency of crude total SLs was better than both distilled water and synthetic surfactants. Furthermore, 83.6% of Cd and 44.8% of Pb were removed by 8% crude acidic SLs. Acidic SLs with high water solubility were more effective than lactonic SLs in enhancing remediation of heavy metal-contaminated soils. The complexation of Cd with the free carboxyl group of the acidic SLs was observed by Fourier-transform infrared spectroscopy study, and this complexation was effective in heavy metal removal from the soil. The fermentation broth of S. bombicola, without further preparation, removed 95% of Cd and 52% of Pb. These results suggested that SLs produced by S. bombicola could function as potential bioremediation agents for heavy metal-contaminated soil.
The Impact of Climate Change on the Food System in Toronto
Authors: Zeuli Kimberly , Nijhuis Austin , Macfarlane Ronald , Ridsdale Taryn
Published: October 2018

As part of its Climate Change and Health Strategy, in 2017, Toronto Public Health engaged stakeholders from across the food system to complete a high-level vulnerability assessment of the impact of climate change on the food system in Toronto. Using the Ontario Climate Change and Health Vulnerability and Adaptation Assessment Guidelines, the City of Toronto’s High-Level Risk Assessment Tool, and a strategic framework developed by the Initiative for a Competitive Inner City, Toronto Public Health identified the most significant extreme weather event risks to food processing, distribution and access in Toronto. Risks associated with three extreme weather events that are the most likely to occur in Toronto due to climate change were analyzed: significant rain and flooding, an extended heat wave, and a major winter ice storm. The analysis finds that while extreme weather events could potentially disrupt Toronto’s food supply, the current risk of an extended, widespread food supply disruption is relatively low. However, the findings highlight that a concerted effort across the food system, including electrical and fuel providers, is needed to address other key vulnerabilities that could impact food access, especially for vulnerable populations. Interruptions to electricity will have food access and food safety impacts, while interruptions to the transportation network and fuel will have food distribution and access impacts. Actions to mitigate these risks could include addressing food access vulnerabilities through ongoing city-wide strategies and integrating food access into the City’s emergency response planning. The next steps will include engaging with multiple partners across the city to understand and strengthen the “last mile” of food distribution and develop community food resilience action plans for vulnerable neighbourhoods.
Making the Case for “Whole System” Approaches: Integrating Public Health and Housing
Authors: Sharpe Richard A. , Taylor Tim , Fleming Lora E. , Morrissey Karyn , Morris George , Wigglesworth Rachel
Published: October 2018

Housing conditions have been an enduring focus for public health activity throughout the modern public health era. However, the nature of the housing and health challenge has changed in response to an evolution in the understanding of the diverse factors influencing public health. Today, the traditional public health emphasis on the type and quality of housing merges with other wider determinants of health. These include the neighbourhood, community, and “place” where a house is located, but also the policies which make access to a healthy house possible and affordable for everyone. Encouragingly, these approaches to policy and action on housing have the potential to contribute to the “triple win” of health and well-being, equity, and environmental sustainability. However, more effective housing policies (and in public health in general) that adopt more systemic approaches to addressing the complex interactions between health, housing, and wider environment are needed. This paper illustrates some of the key components of the housing and health challenge in developed countries, and presents a conceptual model to co-ordinate activities that can deliver the “triple win.” This is achieved by offering a perspective on how to navigate more effectively, inclusively and across sectors when identifying sustainable housing interventions.
Effects of Weir Construction on Phytoplankton Assemblages and Water Quality in a Large River System
Authors: Lee Hae-Jin , Park Hae-Kyung , Cheon Se-Uk
Published: October 2018

Flow regulation is one of the most common anthropogenic factors affecting rivers worldwide. In Korea, 16 weirs were constructed along four major rivers from 2009 to 2012. This study aimed to elucidate initial changes in physical, chemical, and biological variables after the construction of consecutive weirs on the Nakdong River, a major large river system. Water quality variables and phytoplankton cell densities were investigated at eight representative sites and compared with the data recorded before the weir construction. There were spatial and temporal changes in the hydraulic retention time (HRT), total phosphorus (TP), and chlorophyll a concentrations among the eight weir sections. HRT increased after the weir construction, while TP and chlorophyll a tended to decrease from the middle to lower section of the Nakdong River. Furthermore, differences were observed in the phytoplankton community composition between 2006–2007 and 2013. There was a marginal decrease in the duration of centric diatom (Stephanodiscus hantzschii) blooms after weir construction. However, Microcystis aeruginosa proliferated more extensively during summer and autumn than it did before the weir construction. Our results suggest that changes in hydrological factors, in response to consecutive weir construction, may contribute to greater physical, chemical, and ecological variability.
Managerial Areas of Construction and Demolition Waste: A Scientometric Review
Authors: Chen Jianguo , Su Yangyue , Si Hongyun , Chen Jindao
Published: October 2018

In past decades, the massive generation of construction and demolition waste (CDW) was increasingly threatening the public environment and humanity health worldwide. A large amount of research has been devoted to the CDW from difference perspectives. However, few scholars have attempted to summarize and review the extant studies, especially in the managerial areas of CDW (MA-CDW). This paper fills this gap via a systematic and quantitative review in the CDW management field. Employing the scientometric analysis method, a total of 261 articles published from 2006 to 2018 were collected to construct the knowledge map and comprehensive framework for MA-CDW. Results show that the overall evolutionary trend of MA-CDW was from basic management concepts to internal and external challenges analysis, to organizational strategy and innovative management practices. The major MA-CDW knowledge domains were identified and summarized into four pillars, namely: (1) factor and challenge; (2) composition and quantification; (3) assessment and comparison; and (4) technology and method. Based on the trend, knowledge gaps and future research directions were found out and discussed. This study contributes to the existing MA-CDW knowledge by presenting a comprehensive knowledge framework. Furthermore, these findings can provide the researchers and practitioners with an in-depth understanding for the sustainable governance of CDW.
Arachidonic Acid Metabolites in Cardiovascular and Metabolic Diseases
Authors: Sonnweber Thomas , Pizzini Alex , Nairz Manfred , Weiss Günter , Tancevski Ivan
Published: October 2018

Lipid and immune pathways are crucial in the pathophysiology of metabolic and cardiovascular disease. Arachidonic acid (AA) and its derivatives link nutrient metabolism to immunity and inflammation, thus holding a key role in the emergence and progression of frequent diseases such as obesity, diabetes, non-alcoholic fatty liver disease, and cardiovascular disease. We herein present a synopsis of AA metabolism in human health, tissue homeostasis, and immunity, and explore the role of the AA metabolome in diverse pathophysiological conditions and diseases.
The Decrease in Human Endogenous Retrovirus-H Activity Runs in Parallel with Improvement in ADHD Symptoms in Patients Undergoing Methylphenidate Therapy
Authors: Chiara Cipriani , Bernanda Pitzianti Maria , Claudia Matteucci , Elisa D’Agati , Tony Miele Martino , Valentina Rapaccini , Sandro Grelli , Paolo Curatolo , Paola Sinibaldi-Vallebona , Augusto Pasini , Emanuela Balestrieri
Published: October 2018

Increasing scientific evidence demonstrated the deregulation of human endogenous retroviruses (HERVs) expression in complex diseases, such as cancer, autoimmune, psychiatric, and neurological disorders. The dynamic regulation of HERV activity and their responsiveness to a variety of environmental stimuli designate HERVs as genetic elements that could be modulated by drugs. Methylphenidate (MPH) is widely used in the treatment of attention deficit hyperactivity disorder (ADHD). The aim of this study was to evaluate the time course of human endogenous retrovirus H (HERV-H) expression in peripheral blood mononuclear cells (PBMCs) with respect to clinical response in ADHD patients undergoing MPH therapy. A fast reduction in HERV-H activity in ADHD patients undergoing MPH therapy was observed in parallel with an improvement in clinical symptoms. Moreover, when PBMCs from drug-naïve patients were cultured in vitro, HERV-H expression increased, while no changes in the expression levels were found in ADHD patients undergoing therapy. This suggests that MPH could affect the HERV-H activity and supports the hypothesis that high expression levels of HERV-H could be considered a distinctive trait of ADHD patients.
Loss of AMPKα2 Impairs Hedgehog-Driven Medulloblastoma Tumorigenesis
Authors: Zhang Honglai , Kuick Rork , Park Sung-Soo , Peabody Claire , Yoon Justin , Fernández Ester Calvo , Wang Junying , Thomas Dafydd , Viollet Benoit , Inoki Ken , Camelo-Piragua Sandra , Rual Jean-François
Published: October 2018

The AMP-activated protein kinase (AMPK) is a sensor of cellular energy status that has a dual role in cancer, i.e., pro- or anti-tumorigenic, depending on the context. In medulloblastoma, the most frequent malignant pediatric brain tumor, several in vitro studies previously showed that AMPK suppresses tumor cell growth. The role of AMPK in this disease context remains to be tested in vivo. Here, we investigate loss of AMPKα2 in a genetically engineered mouse model of sonic hedgehog (SHH)-medulloblastoma. In contrast to previous reports, our study reveals that AMPKα2 KO impairs SHH medulloblastoma tumorigenesis. Moreover, we performed complementary molecular and genomic analyses that support the hypothesis of a pro-tumorigenic SHH/AMPK/CNBP axis in medulloblastoma. In conclusion, our observations further underline the context-dependent role of AMPK in cancer, and caution is warranted for the previously proposed hypothesis that AMPK agonists may have therapeutic benefits in medulloblastoma patients. Note: an abstract describing the project was previously submitted to the American Society for Investigative Pathology PISA 2018 conference and appears in The American Journal of Pathology (Volume 188, Issue 10, October 2018, Page 2433).
The Small Molecule NLRP3 Inflammasome Inhibitor MCC950 Does Not Alter Wound Healing in Obese Mice
Authors: Lee James S. , Robertson Avril A. B. , Cooper Matthew A. , Khosrotehrani Kiarash
Published: October 2018

The incidence of chronic wounds is escalating, and the associated healing process is especially problematic in an aging population with increased morbidity. Targeting increased inflammation in chronic wounds is a promising but challenging therapeutic strategy. Indeed, inflammation and especially macrophages are required for wound healing. As the NLRP3 inflammasome has been implicated with various other inflammatory diseases, in this study, we used MCC950—a selective NLRP3 small molecule inhibitor—on murine models of both acute and chronic wounds. This molecule, while tested for other inflammatory conditions, has never been investigated to reduce topical inflammation driving chronic wounds. We found that there were no significant differences when the treatment was applied either topically or orally in wild-type C57Bl/6 mice and that it even impaired wound healing in obese mice. The treatment was also unable to improve re-epithelialisation or angiogenesis, which are both required for the closure of wounds. We are inclined to believe that MCC950 may inhibit the closure of chronic wounds and that it does not alter wound-associated macrophage polarisation.
Identification, Structural Characterization and Gene Expression Analysis of Members of the Nuclear Factor-Y Family in Chickpea (Cicer arietinum L.) under Dehydration and Abscisic Acid Treatments
Authors: Chu Ha Duc , Nguyen Kien Huu , Watanabe Yasuko , Le Dung Tien , Pham Thu Ly Thi , Mochida Keiichi , Tran Lam-Son Phan
Published: October 2018

In plants, the Nuclear Factor-Y (NF-Y) transcription factors (TFs), which include three distinct types of NF-YA, NF-YB, and NF-YC TFs, have been identified to play key roles in the regulation of various plant growth and developmental processes under both normal and environmental stress conditions. In this work, a total of 40 CaNF-Y-encoding genes, including eight CaNF-YAs, 21 CaNF-YBs, and 11 CaNF-YCs, were identified in chickpea, and their major gene and protein characteristics were subsequently obtained using various web-based tools. Of our interest, a phylogenetically-based analysis predicted 18 CaNF-Ys (eight CaNF-YAs, seven CaNF-YBs, and three CaNF-YCs) that potentially play roles in chickpea responses to dehydration according to their close relationship with the well-characterized GmNF-Ys in soybean. These results were in good agreement with the enrichment of drought-responsive cis-regulatory motifs and expression patterns obtained from in silico analyses using publically available transcriptome data. Most of the phylogenetically predicted drought-responsive CaNF-Y genes (15 of 18) were quantitatively validated to significantly respond to dehydration treatment in leaves and/or roots, further supporting the results of in silico analyses. Among these CaNF-Y genes, the transcript levels of CaNF-YA01 and CaNF-YC10 were the most highly accumulated in leaves (by approximately eight-fold) and roots (by approximately 18-fold), respectively, by dehydration. Furthermore, 12 of the 18 CaNF-Y genes were found to be responsive to the most well-known stress hormone, namely abscisic acid (ABA), in leaves and/or roots, suggesting that these genes may act in chickpea response to dehydration in ABA-dependent manner. Taken together, our study has provided a comprehensive and fundamental information for further functional analyses of selected CaNF-Y candidate genes, ultimately leading to the improvement of chickpea growth under water-limited conditions.
Structural and Functional Analysis of a Bidirectional Promoter from Gossypium hirsutum in Arabidopsis
Authors: Yang Jiangtao , Wang Xujing , Hasi Agula , Wang Zhixing
Published: October 2018

Stacked traits have become an important trend in the current development of genomically modified crops. The bidirectional promoter can not only prevent the co-suppression of multigene expression, but also increase the efficiency of the cultivation of transgenic plants with multigenes. In Gossypium hirsutum, Ghrack1 and Ghuhrf1 are head-to-head gene pairs located on chromosome D09. We cloned the 1429-bp intergenic region between the Ghrack1 and Ghuhrf1 genes from Gossypium hirsutum. The cloned DNA fragment GhZU had the characteristics of a bidirectional promoter, with 38.7% G+C content, three CpG islands and no TATA-box. Using gfp and gus as reporter genes, a series of expression vectors were constructed into young leaves of tobacco. The histochemical GUS (Beta-glucuronidase) assay and GFP (green fluorescence protein) detection results indicated that GhZU could drive the expression of the reporter genes gus and gfp simultaneously in both orientations. Furthermore, we transformed the expression vectors into Arabidopsis and found that GUS was concentrated at vigorous growth sites, such as the leaf tip, the base of the leaves and pod, and the stigma. GFP was also mainly expressed in the epidermis of young leaves. In summary, we determined that the intergenic region GhZU was an orientation-dependent bidirectional promoter, and this is the first report on the bidirectional promoter from Gossypium hirsutum. Our findings in this study are likely to enhance understanding on the regulatory mechanisms of plant bidirectional promoters.
TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis
Authors: Ke Chih-Chun , Lin Ying-Hung , Wang Ya-Yun , Wu Ying-Yu , Chen Mei-Feng , Ku Wei-Chi , Chiang Han-Sun , Lai Tsung-Hsuan
Published: October 2018

Few papers have focused on small guanosine triphosphate (GTP)-binding proteins and their regulation during spermatogenesis. TBC1D21 genes (also known as male germ cell RAB GTPase-activating protein MGCRABGAP) are related to sterility, as determined through cDNA microarray testing of human testicular tissues exhibiting spermatogenic defects. TBC1D21 is a protein specifically expressed in the testes that exhibits specific localizations of elongating and elongated spermatids during mammalian spermiogenesis. Furthermore, through co-immunoprecipitation (co-IP) and nano liquid chromatography–tandem mass spectrometry (nano LC–MS/MS), Rap1 has been recognized as a potential TBC1D21 interactor. This study determined the possible roles of Rap1 and TBC1D21 during mammalian spermiogenesis. First, the binding ability between Rap1 and TBC1D21 was verified using co-IP. Second, the stronger signals of Rap1 expressed in elongating and elongated murine spermatids extracted from testicular sections, namely spermatogonia, spermatocytes, and round spermatids, were compared. Third, Rap1 and TBC1D21 exhibited similar localizations at postacrosomal regions of spermatids and at the midpieces of mature sperms, through isolated male germ cells. Fourth, the results of an activating Rap1 pull-down assay indicated that TBC1D21 overexpression inactivates Rap1 activity in cell models. In conclusion, TBC1D21 may interact with and potentially regulate Rap1 during murine spermatogenesis.
Voltage-Dependent Sarcolemmal Ion Channel Abnormalities in the Dystrophin-Deficient Heart
Authors: Koenig Xaver , Ebner Janine , Hilber Karlheinz
Published: October 2018

Mutations in the gene encoding for the intracellular protein dystrophin cause severe forms of muscular dystrophy. These so-called dystrophinopathies are characterized by skeletal muscle weakness and degeneration. Dystrophin deficiency also gives rise to considerable complications in the heart, including cardiomyopathy development and arrhythmias. The current understanding of the pathomechanisms in the dystrophic heart is limited, but there is growing evidence that dysfunctional voltage-dependent ion channels in dystrophin-deficient cardiomyocytes play a significant role. Herein, we summarize the current knowledge about abnormalities in voltage-dependent sarcolemmal ion channel properties in the dystrophic heart, and discuss the potentially underlying mechanisms, as well as their pathophysiological relevance.
Sensing of Abiotic Stress and Ionic Stress Responses in Plants
Authors: Zhang Yu , Lv Yang , Jahan Noushin , Chen Guang , Ren Deyong , Guo Longbiao
Published: October 2018

Plants need to cope with complex environments throughout their life cycle. Abiotic stresses, including drought, cold, salt and heat, can cause a reduction in plant growth and loss of crop yield. Plants sensing stress signals and adapting to adverse environments are fundamental biological problems. We review the stress sensors in stress sensing and the responses, and then discuss ionic stress signaling and the responses. During ionic stress, the calcineurin B-like proteins (CBL) and CBL-interacting protein kinases (CBL−CIPK) complex is identified as a primary element of the calcium sensor for perceiving environmental signals. The CBL−CIPK system shows specificity and variety in its response to different stresses. Obtaining a deeper understanding of stress signaling and the responses will mitigate or solve crop yield crises in extreme environments with fast-growing populations.
New Insight into the Molecular Mechanism of the FUT2 Regulating Escherichia coli F18 Resistance in Weaned Piglets
Authors: Wu Zhengchang , Feng Haiyue , Cao Yue , Huang Yanjie , Dai Chaohui , Wu Shenglong , Bao Wenbin
Published: October 2018

Escherichia coli (E. coli) F18 is the main pathogen responsible for post-weaning diarrhea (PWD) in piglets. Resistance to E. coli F18 depends on the expression of the cognate receptors in the intestinal epithelial cells. However, the molecular mechanism of E. coli F18 resistance in weaned piglets remains unclear. Here, we performed a comparative transcriptome study of the duodenal tissue from Sutai E. coli F18 sensitive and resistant pigs by RNA-seq, and pig α(1,2) fucosyltransferase 2 (FUT2) was identified as a host differentially expressed gene controlling the E. coli F18 infection. Function analysis showed that the FUT2 expression was high in the duodenum and jejunum, with higher levels detected in sensitive individuals than in resistant individuals (p < 0.01). Expression levels of FUT2 were upregulated in IPEC-J2 cells after lipopolysaccharide (LPS)-induction or E. coli stimulation. FUT2 knockdown decreased the adhesion of E. coli F18 to IPEC-J2 cells (p < 0.05). FUT2 overexpression markedly increased the adhesion of E. coli F18 to IPEC-J2 cells (p < 0.05 or p < 0.01). Furthermore, the FUT2 mRNA levels correlated with methylation levels of the mC-22 site in the specificity protein 1 (Sp1) transcription factor (p < 0.05). Electrophoretic mobility shift assays (EMSA) showed that Sp1 interacts with the wild-type FUT2 promoter DNA, but not with methylated DNA. Our data suggested that FUT2 methylation at the mC-22 site inhibits Sp1 binding to the FUT2 promoter, thereby reducing FUT2 expression and enhancing E. coli F18 resistance in weaned piglets. These observations highlight FUT2 as a promising new target for combating E. coli F18 susceptibility in weaned piglets.
Increased Plasma Circulating Cell-Free DNA Could Be a Potential Marker for Oral Cancer
Authors: Lin Li-Han , Chang Kuo-Wei , Kao Shou-Yen , Cheng Hui-Wen , Liu Chung-Ji
Published: October 2018

Background: Oral squamous cell carcinoma (OSCC) is a disease that affects patients worldwide. DNA of dead cells is released into the blood stream and may be isolated from plasma or serum samples. This DNA is termed cell-free DNA (cfDNA). cfDNA is increased in several types of malignancies. We investigated if there was a correlation between cfDNA levels and the progression of OSCC. Methods: Using quantitative spectrometry, we measured plasma cfDNA in 121 patients with OSCC and 50 matched controls. Mann Whitney and Wilcoxon tests were used to compare differences among various clinical variants. Receiver operating characteristic (ROC) analysis was used to obtain levels suitable for the separation of the clinical subsets. Kaplan-Meier analysis was used to assess correlation with survival. Results: Plasma cfDNA was significantly elevated in patients with OSCC relative to controls. Plasma cfDNA levels correlated with larger tumor size, cervical lymph node metastasis and late stage. Higher plasma cfDNA levels were associated with a poor prognosis of OSCC, which is a new finding. Conclusion: Plasma cfDNA could serve as a novel and easily accessible biomarker in OSCC, providing diagnostic and prognostic value.
A Recombinant Affinity Reagent Specific for a Phosphoepitope of Akt1
Authors: McGinnis Jennifer E. , Venegas Leon A. , Lopez Hector , Kay Brian K.
Published: October 2018

The serine/threonine-protein kinase, Akt1, plays an important part in mammalian cell growth, proliferation, migration and angiogenesis, and becomes activated through phosphorylation. To monitor phosphorylation of threonine 308 in Akt1, we developed a recombinant phosphothreonine-binding domain (pTBD) that is highly selective for the Akt1 phosphopeptide. A phage-display library of variants of the Forkhead-associated 1 (FHA1) domain of yeast Rad53p was screened by affinity selection to the phosphopeptide, 301-KDGATMKpTFCGTPEY-315, and yielded 12 binding clones. The strongest binders have equilibrium dissociation constants of 160–180 nanomolar and are phosphothreonine-specific in binding. The specificity of one Akt1-pTBD was compared to commercially available polyclonal antibodies (pAbs) generated against the same phosphopeptide. The Akt1-pTBD was either equal to or better than three pAbs in detecting the Akt1 pT308 phosphopeptide in ELISAs.
Serratia liquefaciens KM4 Improves Salt Stress Tolerance in Maize by Regulating Redox Potential, Ion Homeostasis, Leaf Gas Exchange and Stress-Related Gene Expression
Authors: El-Esawi Mohamed A. , Alaraidh Ibrahim A. , Alsahli Abdulaziz A. , Alzahrani Saud M. , Ali Hayssam M. , Alayafi Aisha A. , Ahmad Margaret
Published: October 2018

High salinity mitigates crop productivity and quality. Plant growth-promoting soil rhizobacteria (PGPR) improve plant growth and abiotic stress tolerance via mediating various physiological and molecular mechanisms. This study investigated the effects of the PGPR strain Serratia liquefaciens KM4 on the growth and physiological and molecular responsiveness of maize (Zea mays L.) plants under salinity stress (0, 80, and 160 mM NaCl). High salinity significantly reduced plant growth and biomass production, nutrient uptake, leaf relative water content, pigment content, leaf gas exchange attributes, and total flavonoid and phenolic contents in maize. However, osmolyte content (e.g., soluble proteins, proline, and free amino acids), oxidative stress markers, and enzymatic and non-enzymatic antioxidants levels were increased in maize under high salinity. On the other hand, Serratia liquefaciens KM4 inoculation significantly reduced oxidative stress markers, but increased the maize growth and biomass production along with better leaf gas exchange, osmoregulation, antioxidant defense systems, and nutrient uptake under salt stress. Moreover, it was found that all these improvements were accompanied with the upregulation of stress-related genes (APX, CAT, SOD, RBCS, RBCL, H+-PPase, HKT1, and NHX1), and downregulation of the key gene in ABA biosynthesis (NCED). Taken together, the results demonstrate the beneficial role of Serratia liquefaciens KM4 in improving plant growth and salt stress tolerance in maize by regulating ion homeostasis, redox potential, leaf gas exchange, and stress-related genes expression.
Remnant Lipoprotein Cholesterol and Cardiovascular and Cerebrovascular Events in Patients with Non-Alcoholic Fatty Liver Disease
Authors: Pastori Daniele , Baratta Francesco , Novo Marta , Cocomello Nicholas , Violi Francesco , Angelico Francesco , Del Ben Maria
Published: October 2018

Non-alcoholic fatty liver disease (NAFLD) is characterized by an atherogenic dyslipidaemia and an increased cardiovascular risk. Remnant lipoprotein cholesterol (RLP-C) is emerging as a novel cardiovascular risk factor, but its predictive value in patients with NAFLD is unknown. We investigated factors affecting RLP-C levels, and the association with major adverse cardiovascular and cerebrovascular events (MACCE) in NAFLD. A prospective observational cohort study was carried out including 798 unselected patients with cardio-metabolic diseases screened by ultrasound for the presence of NAFLD. Fasting RLP-C (mg/dL) was calculated as total cholesterol—(HDL (high-density lipoprotein) + LDL (low-density-lipoprotein)). Primary endpoint of the follow-up study was a combined endpoint of MACCE. Patients with NAFLD (79.2%) had higher median fasting RLP-C in comparison to those without (27.0 vs. 20.0 mg/dL, respectively p < 0.001). Metabolic syndrome, NAFLD, age above median, and female sex were independently associated to fasting RLP-C above the median. In patients with NAFLD, values of RLP-C were associated with liver disease severity, as shown by the increasing value of RLP-C across tertiles of aspartate aminotransferase (AST) (p = 0.002) and gamma-glutamyl transpeptidase (GGT) (p < 0.001). Furthermore, levels of RLP-C and Hamaguchi score, were significantly correlated (r = 0.193, p < 0.001). During a median follow-up of 32 months (interquartile range: 14.2–51.7, 1700 person-years), 41 MACCE (2.41%/year) were registered in 596 NAFLD patients. The rate of events was higher in NAFLD patients with RLP-C above the median compared to those below (log-rank test p = 0.040). Age (hazard ratio (HR) 1.039, 95% confidence interval (CI), 1.005–1.074, p = 0.024), previous cardiovascular events (HR 2.210, 95% CI, 1.052–4.643, p = 0.036), female sex (HR 0.454, 95% CI, 0.208–0.989, p = 0.047) and RLP-C above the median (HR 2.202, 95% CI, 1.132–4.285, p = 0.020) were associated with MACCE. In conclusion, we found that NAFLD was independently associated with higher circulating RLP-C, and that high RLP-C levels were predictive of MACCE in patients with NAFLD.