bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrochemical Detection of H2O2 Released from Prostate Cancer Cells Using Pt Nanoparticle-Decorated rGO–CNT Nanocomposite-Modified Screen-Printed Carbon Electrodes
Authors: Lee Seokyung , Lee Young Ju , Kim Jae Hyung , Lee Gi-Ja
Published: August 2020

In this study, we fabricated platinum nanoparticles (PtNP)-decorated, porous reduced graphene oxide (rGO)–carbon nanotube (CNT) nanocomposites on a PtNP-deposited screen-printed carbon electrode (PtNP/rGO–CNT/PtNP/SPCE) for detection of hydrogen peroxide (H2O2), which is released from prostate cancer cells LNCaP. The PtNP/rGO–CNT/PtNP/SPCE was fabricated by a simple electrochemical deposition and co-reduction method. In addition, the amperometric response of the PtNP/rGO–CNT/PtNP/SPCE electrode was evaluated through consecutive additions of H2O2 at an applied potential of 0.2 V (vs. Ag pseudo-reference electrode). As a result, the prepared PtNP/rGO–CNT/PtNP/SPCE showed good electrocatalytic activity toward H2O2 compared to bare SPCE, rGO–CNT/SPCE, PtNP/SPCE, and rGO–CNT/PtNP/SPCE. In addition, the PtNP/rGO–CNT/PtNP/SPCE electrode exhibited a sensitivity of 206 μA mM−1·cm−2 to H2O2 in a linear range of 25 to 1000 μM (R2 = 0.99). Moreover, the PtNP/rGO–CNT/PtNP/SPCE electrode was less sensitive to common interfering substances, such as ascorbic acid, uric acid, and glucose than H2O2. Finally, real-time monitoring of H2O2 released from LNCaP cells was successfully performed by this electrode. Therefore, we expect that the PtNP/rGO–CNT/PtNP/SPCE can be utilized as a promising electrochemical sensor for practical nonenzymatic detection of H2O2 in live cells or clinical analysis.
A Single Liver Metastasis from Pleural Biphasic Mesothelioma
Authors: Marzullo Andrea , Serio Gabriella , Pezzuto Federica , Fortarezza Francesco , Cazzato Gerardo , Caporusso Concetta , Lettini Teresa , Cavone Domenica , Delfino Maria Celeste , Vimercati Luigi
Published: August 2020

Virtually any malignancy can metastasize to the liver. Large solitary metastases are rare and can be difficult to distinguish from primary tumors. Malignant mesothelioma is often considered as a locally invasive cancer but tumor dissemination to extra-thoracic sites is possible, and the liver can be involved. Herein, we present a rare case of pleural mesothelioma with a solitary large liver metastasis diagnosed postmortem in a ninety-two-year-old man with 35 years of exposure to asbestos. Results of immunohistochemical staining of the pleural and liver tumor were similar, both positive for low-molecular weight keratins, calretinin, vimentin, and podoplanin, and negative for Claudin-4, TTF1, CEA, BerEP4, CK7, CK19, CK20, BAP1, Hep Par1, p40, and WT1. Fluorescent in-situ hybridization (FISH) for p16/CDKN2A was also performed and a homozygous deletion was detected in both tumors, supporting the diagnosis of mesothelioma. Reporting this case, we would like to point out that extra-thoracic dissemination from pleural mesothelioma, even if exceptional, can occur. In cases where differential diagnoses are challenging, the value of ancillary techniques and a practical approach to diagnostic work-up is of primary importance.
Design of an Equivalent Consumption Minimization Strategy-Based Control in Relation to the Passenger Number for a Fuel Cell Tram Propulsion
Authors: Piraino Francesco , Fragiacomo Petronilla
Published: August 2020

In this paper, a new control strategy for a fuel cell supercapacitor tramway is tested on a real drive cycle. The control algorithm is based on the equivalent consumption minimization strategy, weighted for the vehicle passenger number. Its implementation is presented by highlighting the customization for a specific drive cycle, located in Reggio Calabria, one of the main cities of southern Italy. The heart of the paper concerns the fuel cell hybrid powertrain, where energy source and DC/DC converter models are formalized and numerically designed; in addition, all the drivetrain components are taken into account through appropriate relations. By means of the drive cycle characteristics, in terms of morphology, vehicle features, and speed, the main components are properly selected, with the aim of avoiding under- and over-sizing issues. A specific case study is analyzed, considering the passenger variation at each tramway stop. Satisfying results are achieved in the simulation campaign: 2.9 kg of hydrogen is consumed for a round trip, with a quasi-constant fuel cell efficiency of more than 50%, while the supercapacitor SOC ranges in a wide interval, between 35% and 95%.
Optimal Management of the Desalination System Demand in Non-Interconnected Islands
Authors: Karakitsios Ioannis , Dimeas Aris , Hatziargyriou Nikos
Published: August 2020

The high energy consumption of desalination systems represents a significant opportunity for the application of demand response schemes, particularly for the case of Non-Interconnected Island (NII) systems. In particular, the optimal management of the desalination demand can be proven quite beneficial in increasing the Renewable Energy Sources’ (RES) penetration, which is one of the main objectives in the day-ahead scheduling of the electricity system in Greek NIIs. This paper proposes a detailed representation of the desalination system, taking into account all the relevant constraints for the system’s operation. The mathematical representation of the aforementioned operation is incorporated in the day-ahead scheduling (DAS) for the case of Greek NII systems in order to define the optimal operational scheduling of a desalination system. The proposed optimisation procedure is applied for the desalination system installed in the Greek island of Kythnos. The results of the analysis indicate that the DAS problem shall be fully aware of the capabilities of the desalination system in order to allow specific water flows (in and out of specific reservoirs) at specific hours of the day, allowing the optimal exploitation of the available RES produced energy.
Recent Advances in the Critical Heat Flux Amelioration of Pool Boiling Surfaces Using Metal Oxide Nanoparticle Deposition
Authors: Moghadasi Hesam , Malekian Navid , Saffari Hamid , Mirza Gheitaghy Amir , Zhang Guo Qi
Published: August 2020

Pool boiling is an effective heat transfer process in a wide range of applications related to energy conversion, including power generation, solar collectors, cooling systems, refrigeration and air conditioning. By considering the broad range of applications, any improvement in higher heat-removal yield can ameliorate the ultimate heat usage and delay or even avoid the occurrence of system failures, thus leading to remarkable economic, environmental and energy efficiency outcomes. A century of research on ameliorating critical heat flux (CHF) has focused on altering the boiling surface characteristics, such as its nucleation site density, wettability, wickability and heat transfer area, by many innovative techniques. Due to the remarkable interest of using nanoparticle deposition on boiling surfaces, this review is targeted towards investigating whether or not metal oxide nanoparticles can modify surface characteristics to enhance the CHF. The influence of nanoparticle material, thermo-physical properties, concentration, shape, and size are categorized, and the inconsistency or contradictions of the existing research results are recognized. In the following, nanoparticle deposition methods are presented to provide a worthwhile alternative to deposition rather than nanofluid boiling. Furthermore, possible mechanisms and models are identified to explain the amelioration results. Finally, the present status of nanoparticle deposition for CHF amelioration, along with their future challenges, amelioration potentials, limitations, and their possible industrial implementation, is discussed.
Detailing the Self-Discharge of a Cathode Based on a Prussian Blue Analogue
Authors: Musella Elisa , Mullaliu Angelo , Ruf Thomas , Huth Paula , Tonelli Domenica , Aquilanti Giuliana , Denecke Reinhard , Giorgetti Marco
Published: August 2020

Prussian Blue analogues (PBAs) are a promising class of electrode active materials for batteries. Among them, copper nitroprusside, Cu[Fe(CN)5NO], has recently been investigated for its peculiar redox system, which also involves the nitrosyl ligand as a non-innocent ligand, in addition to the electroactivity of the metal sites, Cu and Fe. This paper studies the dynamics of the electrode, employing surface sensitive X-ray Photoelectron spectroscopy (XPS) and bulk sensitive X-ray absorption spectroscopy (XAS) techniques. XPS provided chemical information on the layers formed on electrode surfaces following the self-discharge process of the cathode material in the presence of the electrolyte. These layers consist mainly of electrolyte degradation products, such as LiF, LixPOyFz and LixPFy. Moreover, as evidenced by XAS and XPS, reduction at both metal sites takes place in the bulk and in the surface of the material, clearly evidencing that a self-discharge process is occurring. We observed faster processes and higher amounts of reduced species and decomposition products in the case of samples with a higher amount of coordination water.
Design, Implementation, and Evaluation of Open Power Quality
Authors: Christe Anthony J. , Negrashov Sergey , Johnson Philip M.
Published: August 2020

Modern electrical grids are transitioning from a centralized generation architecture to an architecture with significant distributed, intermittent generation. This transition means that the formerly sharp distinction between energy producers (utility companies) and consumers (residences, businesses, etc.) are blurring: end-users both produce and consume energy, making energy management and public policy more complex. The goal of the Open Power Quality (OPQ) project is to design and implement a low cost, distributed power quality sensor network that provides useful new forms of information about modern electrical grids to producers, consumers, researchers, and policy makers. In 2019, we performed a pilot study involving the deployment of an OPQ sensor network at the University of Hawaii microgrid for three months. Results of the pilot study validate the ability of OPQ to collect accurate power quality data in a way that provides useful new insights into electrical grids.
Flammability Characteristics of Surface Fuels in a Longleaf Pine (Pinus palustris Mill.) Woodland
Authors: Emery Raien K. , Hart Justin L.
Published: August 2020

To investigate fuel flammability, we quantified burning characteristics of 21 fuel categories in a longleaf pine (Pinus palustris Mill.) woodland in central Alabama, USA. Litter was burned under controlled laboratory conditions. Flammability characteristics, including resistance to ignition, flaming duration, smoldering duration, maximum flame height, and percent consumption, were measured. The fuels were hierarchically clustered into five groups of similar flammability characteristics that explained 89% of the variance. Percent consumption and maximum flame height values ranged from 7% ± 1 standard errors (SE) and 12 cm ± 1 SE for the low flammability group (bark and charcoal), to 86% ± 1 SE and 62 cm ± 3 SE for the high flammability group (bracken fern (Pteridium latiusculum (Desv.) Hieron. ex R.E.Fr. = {syn: P. aquilinum}), grass, and fire-facilitating oak (Quercus spp.) leaves). Results support observed flammability differences between fuel types such as oak and pine (Pinus spp.) litter, and duff, and provide a previously unquantified comparison of surface fuels comprehensive of a longleaf pine community. Further, clustering analysis indicated that plant species that become abundant post-disturbance may help maintain fire-vegetation feedbacks in the absence of pine litter. Understanding flammability characteristics of surface fuels may further inform prescribed fire application in stands where fuels have been altered.
Meat Quality Parameters and Sensory Properties of One High-Performing and Two Local Chicken Breeds Fed with Vicia faba
Authors: Escobedo del Bosque Cynthia I. , Altmann Brianne A. , Ciulu Marco , Halle Ingrid , Jansen Simon , Nolte Tanja , Weigend Steffen , Mörlein Daniel
Published: August 2020

The current practices of the poultry industry have raised concerns among consumers. Among these is the culling of day-old male chicks of laying hybrids; a suitable alternative for this could be the use of dual-purpose breeds where both sexes are used. Another practice that causes concern is the import of large quantities of soybeans for feedstuff production. Substitutes for these soybean-based products are regional protein crops, such as faba beans (Vicia faba L.; FBs). The objective of this study was to test the suitability of FB as a locally produced soybean meal replacement for two local dual-purpose chicken breeds and one high-performing layer line. The breast and leg meat of male Bresse Gauloise (BG), Vorwerkhuhn (VH), and White Rock (WR) animals was evaluated for different meat quality parameters: pH, color, water holding capacity, and tenderness. Sensory properties of the samples were evaluated by a trained panel with a conventional descriptive analysis. Results show different effects of FB diets on meat quality parameters in the different breeds. The attributes mostly affected by the diet are related to aroma, flavor, and texture, particularly in VH and WR. Overall, faba beans appear to be an acceptable dietary protein source for rearing these breeds for meat production.
Motivating Military Trainee Healthy Eating: Insight from Two Sites
Authors: Kitunen Anna , Carins Julia , Rundle-Thiele Sharyn
Published: August 2020

This paper investigates eating behaviours and motivations of early career military trainees from two pathways (officer cadets and army recruits) to understand whether, and to what extent, healthful eating behaviours were present, and what motivates eating in general and healthful habits specifically. The study also sought to understand whether groups need strategies that are pathway specific or are transferrable across different trainee populations. Participants were recruited via email to complete an online survey and through in-person invitations to ensure a diverse cross section of trainees (n = 195) and recruits (n = 297). Two-step cluster analysis revealed three distinct segments with education, opportunity and motivation being the most important variables within a wider multivariate segment formation and stepwise linear regression was performed to identify the most optimal model with the least number of predictors for each segment. The ideal model for the uninterested segment contained nine predictors, seven predictors for the Breakfast skippers segment and eight predictors for the Weight conscious segment. This study found that there is room for improvement in the eating habits of military trainees across military training pathways. Eating motivations, and their associations with healthful eating habits, indicate a need for strategies that are broader than instilling or reinforcing health motivations. Strategies that can be implemented to support healthful eating for military trainees include provision of food preparation and cooking skills training, coupled with provision of social support and environments that facilitate healthful eating. Furthermore, three distinct segments were discovered within the trainee population, indicating that strategies for positively changing trainees’ eating behaviours may not need to be pathway specific, rather it may be possible to introduce the same group of segmented strategies across both pathways. Future research directions and limitations are outlined.
Schoolteachers’ Knowledge of Attention-Deficit/Hyperactivity Disorder—Current Status and Effectiveness of Knowledge Improvement Program: A Randomized Controlled Trial
Authors: Alshehri Abdullah M. , Shehata Shehata F. , Almosa Khalid M. , Awadalla Nabil J.
Published: August 2020

Background: Adequately knowledgeable schoolteachers can play an essential role in early detection and proper treatment of children with attention-deficit/hyperactivity disorder (ADHD) at school. Objectives: To assess the schoolteachers’ knowledge of ADHD and to evaluate the effectiveness of the ADHD knowledge improvement program. Methods: A randomized controlled trial study design was followed on 100 primary school teachers from Abha City, Saudi Arabia. Teachers were randomly selected and allocated into trial and control groups. A self-administered questionnaire was used to evaluate teachers’ knowledge about ADHD. After the baseline assessment, participants in the trial group received a two-day ADHD knowledge improvement program. Teachers’ knowledge about ADHD was reassessed using the same questionnaire immediately after finishing the intervention program and after three months in both groups. Results: Only 16% and 22% of trial and control groups, respectively, showed adequate baseline knowledge about ADHD. Teachers’ main sources of information about ADHD were the internet (67%), friends (47%), TV (34%), and reading books (23%). Knowledge of teachers in the trial group significantly improved immediately after the intervention. After three months, these knowledge benefits slightly declined but remained more adequate than those of teachers in the control group. The logistic regression model revealed that being a participant in the trial group and of higher qualification were associated with significant improvement in teachers’ retained knowledge on ADHD (p < 0.001 and p = 0.050, respectively). Conclusions: The majority of male primary school teachers in Abha City, Saudi Arabia, have inadequate knowledge about ADHD. Applying a knowledge improvement program can substantially improve their knowledge of ADHD. Consideration should be given to the integration of ADHD knowledge improvement program into teachers’ educational programs.
Design and Validation of the Adaptation to Change Questionnaire: New Realities in Times of COVID-19
Authors: Pérez-Fuentes María del Carmen , Molero Jurado María del Mar , Martos Martínez África , Fernández-Martínez Elena , Franco Valenzuela Raquel , Herrera-Peco Iván , Jiménez-Rodríguez Diana , Méndez Mateo Inmaculada , Santillán García Azucena , Simón Márquez María del Mar , Gázquez Linares José Jesús
Published: August 2020

Emotional and cognitive-behavioral factors influence people’s adaptability to change. Based on this premise, the objective of this study was to develop, evaluate and validate the Adaptation to Change Questionnaire (ADAPTA-10) for identifying those who show poor adaptability to adverse situations, such as those caused by COVID-19. This study was carried out in a sample of 1160 adults and produced a 10-item instrument with good reliability and validity indices. It is an effective tool useful in research and in clinical practice. Calculation tables are provided for the general Spanish population and by sex to evaluate adaptability to change. The two-dimensional structure proposed in the original model was confirmed. This instrument will enable the needs for adaptation to the new reality associated with COVID-19 to be detected and also other situations in which the subject becomes immersed which demand adaptation strategies in the new situation lived in.
A Look Behind the Scenes at COVID-19: National Strategies of Infection Control and Their Impact on Mortality
Authors: Haj Bloukh Samir , Edis Zehra , Shaikh Annis A. , Pathan Habib M.
Published: August 2020

(1) Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began spreading across the globe in December and, as of 9 July 2020, had inflicted more than 550,000 deaths. Public health measures implemented to control the outbreak caused socio-economic havoc in many countries. The pandemic highlighted the quality of health care systems, responses of policymakers in harmony with the population, and socio-economic resilience factors. We suggest that different national strategies had an impact on mortality and case count. (2) Methods: We collected fatality data for 17 countries until 2 June 2020 from public data and associated these with implemented containment measures. (3) Results: The outcomes present the effectiveness of control mechanisms in mitigating the virus for selected countries and the UAE as a special case. Pre-existing conditions defined the needed public health strategies and fatality numbers. Other pre-existing conditions, such as temperature, humidity, median age, and low serum 25-hydroxyvitamin D (25(OH)D) concentrations played minor roles and may have had no direct impact on fatality rates. (4) Conclusions: Prevention, fast containment, adequate public health strategies, and importance of indoor environments were determining factors in mitigating the pandemic. Development of public health strategies adapted to pre-existing conditions for each country and community compliance with implemented policies ensure the successful control of pandemics.
Synaptic Organization of the Human Temporal Lobe Neocortex as Revealed by High-Resolution Transmission, Focused Ion Beam Scanning, and Electron Microscopic Tomography
Authors: Rollenhagen Astrid , Walkenfort Bernd , Yakoubi Rachida , Klauke Sarah A. , Schmuhl-Giesen Sandra F. , Heinen-Weiler Jacqueline , Voortmann Sylvia , Marshallsay Brigitte , Palaz Tayfun , Holz Ulrike , Hasenberg Mike , Lübke Joachim H.R.
Published: August 2020

Modern electron microscopy (EM) such as fine-scale transmission EM, focused ion beam scanning EM, and EM tomography have enormously improved our knowledge about the synaptic organization of the normal, developmental, and pathologically altered brain. In contrast to various animal species, comparably little is known about these structures in the human brain. Non-epileptic neocortical access tissue from epilepsy surgery was used to generate quantitative 3D models of synapses. Beside the overall geometry, the number, size, and shape of active zones and of the three functionally defined pools of synaptic vesicles representing morphological correlates for synaptic transmission and plasticity were quantified. EM tomography further allowed new insights in the morphological organization and size of the functionally defined readily releasable pool. Beside similarities, human synaptic boutons, although comparably small (approximately 5 µm), differed substantially in several structural parameters, such as the shape and size of active zones, which were on average 2 to 3-fold larger than in experimental animals. The total pool of synaptic vesicles exceeded that in experimental animals by approximately 2 to 3-fold, in particular the readily releasable and recycling pool by approximately 2 to 5-fold, although these pools seemed to be layer-specifically organized. Taken together, synaptic boutons in the human temporal lobe neocortex represent unique entities perfectly adapted to the “job” they have to fulfill in the circuitry in which they are embedded. Furthermore, the quantitative 3D models of synaptic boutons are useful to explain and even predict the functional properties of synaptic connections in the human neocortex.
Contribution of P2X4 Receptors to CNS Function and Pathophysiology
Authors: Montilla Alejandro , Mata Gilda Paloma , Matute Carlos , Domercq Maria
Published: August 2020

The release and extracellular action of ATP are a widespread mechanism for cell-to-cell communication in living organisms through activation of P2X and P2Y receptors expressed at the cell surface of most tissues, including the nervous system. Among ionototropic receptors, P2X4 receptors have emerged in the last decade as a potential target for CNS disorders such as epilepsy, ischemia, chronic pain, anxiety, multiple sclerosis and neurodegenerative diseases. However, the role of P2X4 receptor in each pathology ranges from beneficial to detrimental, although the mechanisms are still mostly unknown. P2X4 is expressed at low levels in CNS cells including neurons and glial cells. In normal conditions, P2X4 activation contributes to synaptic transmission and synaptic plasticity. Importantly, one of the genes present in the transcriptional program of myeloid cell activation is P2X4. Microglial P2X4 upregulation, the P2X4+ state of microglia, seems to be common in most acute and chronic neurodegenerative diseases associated with inflammation. In this review, we summarize knowledge about the role of P2X4 receptors in the CNS physiology and discuss potential pitfalls and open questions about the therapeutic potential of blocking or potentiation of P2X4 for different pathologies.
Dephosphorylation of LjMPK6 by Phosphatase LjPP2C is Involved in Regulating Nodule Organogenesis in Lotus japonicus
Authors: Yan Zhongyuan , Cao Jingjing , Fan Qiuling , Chao Hongmin , Guan Xiaomin , Zhang Zhongming , Duanmu Deqiang
Published: August 2020

The mitogen-activated protein kinase (MAPK) LjMPK6 is a phosphorylation target of SIP2, a MAPK kinase that interacts with SymRK (symbiosis receptor-like kinase) for regulation of legume-rhizobia symbiosis. Both LjMPK6 and SIP2 are required for nodulation in Lotus japonicus. However, the dephosphorylation of LjMPK6 and its regulatory components in nodule development remains unexplored. By yeast two-hybrid screening, we identified a type 2C protein phosphatase, LjPP2C, that specifically interacts with and dephosphorylates LjMPK6 in vitro. Physiological and biochemical assays further suggested that LjPP2C phosphatase is required for dephosphorylation of LjMPK6 in vivo and for fine-tuning nodule development after rhizobial inoculation. A non-phosphorylatable mutant variant LjMPK6 (T224A Y226F) could mimic LjPP2C functioning in MAPK dephosphorylation required for nodule development in hairy root transformed plants. Collectively, our study demonstrates that interaction with LjPP2C phosphatase is required for dephosphorylation of LjMPK6 to fine tune nodule development in L. japonicus.
Mutation in Sodium-Glucose Cotransporter 2 Results in Down-Regulation of Amyloid Beta (A4) Precursor-Like Protein 1 in Young Age, Which May Lead to Poor Memory Retention in Old Age
Authors: Unno Keiko , Takagi Yoshiichi , Konishi Tomokazu , Suzuki Mitsuhiro , Miyake Akiyuki , Kurotaki Takumi , Hase Tadashi , Meguro Shinichi , Shimada Atsuyoshi , Hasegawa-Ishii Sanae , Pervin Monira , Taguchi Kyoko , Nakamura Yoriyuki
Published: August 2020

Senescence-accelerated mouse prone 10 (SAMP10) exhibits cerebral atrophy and depression-like behavior. A line of SAMP10 with spontaneous mutation in the Slc5a2 gene encoding the sodium-glucose cotransporter (SGLT) 2 was named SAMP10/TaSlc-Slc5a2slc (SAMP10-ΔSglt2) and was identified as a renal diabetes model. In contrast, a line of SAMP10 with no mutation in SGLT2 (SAMP10/TaIdrSlc, SAMP10(+)) was recently established under a specific pathogen-free condition. Here, we examined the mutation effect in SGLT2 on brain function and longevity. No differences were found in the survival curve, depression-like behavior, and age-related brain atrophy between SAMP10-ΔSglt2 and SAMP10(+). However, memory retention was lower in SAMP10-ΔSglt2 mice than SAMP10(+). Amyloid beta (A4) precursor-like protein 1 (Aplp1) expression was significantly lower in the hippocampus of SAMP10-ΔSGLT2 than in SAMP10(+) at 2 months of age, but was similar at 12 months of age. CaM kinase-like vesicle association (Camkv) expression was remarkably lower in SAMP10(+). These genes have been reported to be involved in dendrite function. Amyloid precursor proteins have been reported to involve in maintaining homeostasis of glucose and insulin. These results suggest that mutation in SGLT2 results in down-regulation of Aplp1 in young age, which can lead to poor memory retention in old age.
Optimized Protocol for Isolation of Small Extracellular Vesicles from Human and Murine Lymphoid Tissues
Authors: Bordas Marie , Genard Géraldine , Ohl Sibylle , Nessling Michelle , Richter Karsten , Roider Tobias , Dietrich Sascha , Maaß Kendra K. , Seiffert Martina
Published: August 2020

Small extracellular vesicles (sEVs) are nanoparticles responsible for cell-to-cell communication released by healthy and cancer cells. Different roles have been described for sEVs in physiological and pathological contexts, including acceleration of tissue regeneration, modulation of tumor microenvironment, or premetastatic niche formation, and they are discussed as promising biomarkers for diagnosis and prognosis in body fluids. Although efforts have been made to standardize techniques for isolation and characterization of sEVs, current protocols often result in co-isolation of soluble protein or lipid complexes and of other extracellular vesicles. The risk of contaminated preparations is particularly high when isolating sEVs from tissues. As a consequence, the interpretation of data aiming at understanding the functional role of sEVs remains challenging and inconsistent. Here, we report an optimized protocol for isolation of sEVs from human and murine lymphoid tissues. sEVs from freshly resected human lymph nodes and murine spleens were isolated comparing two different approaches—(1) ultracentrifugation on a sucrose density cushion and (2) combined ultracentrifugation with size-exclusion chromatography. The purity of sEV preparations was analyzed using state-of-the-art techniques, including immunoblots, nanoparticle tracking analysis, and electron microscopy. Our results clearly demonstrate the superiority of size-exclusion chromatography, which resulted in a higher yield and purity of sEVs, and we show that their functionality alters significantly between the two isolation protocols.
Adaptation and Validation of the Diabetic Foot Ulcer Scale-Short Form in Spanish Subjects
Authors: Martinez-Gonzalez Dolores , Dòria Montserrat , Martínez-Alonso Montserrat , Alcubierre Nuria , Valls Joan , Verdú-Soriano José , Granado-Casas Minerva , Mauricio Didac
Published: August 2020

Diabetic foot ulcer (DFU) is a chronic complication that negatively affects the quality of life (QoL) of diabetic patients. In Spain, there is no specifically designed and validated instrument to assess the QoL of patients with DFU. Our aim was to adapt the Diabetic Foot Ulcer Scale-Short Form (DFS-SF) questionnaire to a Spanish population and validate it. A prospective, observational design was used. The DFS-SF was administered by personal interview. The validated SF-36 and EQ-5D generic instruments were used as reference tools. The reliability, validity, and sensitivity to changes were assessed using standard statistical methods. A sample of 141 patients with DFU was recruited. The content validity was 3.46 on average (maximum score of 4). The internal consistency of the DFS-SF subscales showed a standardized Cronbach’s α range between 0.720 and 0.948. The DFS-SF domains showed excellent reproducibility measures (intraclass correlation coefficient from 0.77–0.92). The criterion validity was good with significant correlations between each DFS-SF subscale and its corresponding SF-36 and EQ-5D subscales (p < 0.001). However, the questionnaire structure was not validated (comparative fit index = 0.844, root mean square error of approximation = 0.095, and standardized root mean square residual = 0.093). The instrument showed high sensitivity to ulcer changes over time (p < 0.001). The adapted and validated Spanish version of the DFS-SF questionnaire has good psychometric properties and shows good sensitivity to ulcer changes, although the construct validity was not optimal. The adapted questionnaire will be a useful tool specifically to assess the QoL in subjects with diabetic foot ulcers in the clinical and research settings in Spain.
Isolated Pubic Ramus Fractures Are Serious Adverse Events for Elderly Persons: An Observational Study on 138 Patients with Fragility Fractures of the Pelvis Type I (FFP Type I)
Authors: Rommens Pol Maria , Hopf Johannes Christof , Herteleer Michiel , Devlieger Benjamin , Hofmann Alexander , Wagner Daniel
Published: August 2020

Background: Fractures of the pubic ramus without involvement of the posterior pelvic ring represent a minority of fragility fractures of the pelvis (FFP). The natural history of patients suffering this FFP Type I has not been described so far. Material and methods: All patients, who were admitted with isolated pubic ramus fractures between 2007 and mid-2018, have been reviewed. Epidemiologic data, comorbidities, in-hospital complications, and one-year mortality were recorded. Of all surviving patients, living condition before the fracture and at follow-up was noted. Mobility was scored with the Parker Mobility Score, quality of life with the European Quality of Life 5 Dimensions 3 Level (EQ-5D-3L), subjective sensation of pain with the Numeric Rating Scale (NRS). Results: A consecutive series of 138 patients was included in the study. There were 117 women (84.8%) and 21 men (15.2%). Mean age was 80.6 years (SD 8.6 years). 89.1% of patients presented with comorbidities, 81.2% of them had cardiovascular diseases. Five patients (4%) died during hospital-stay. Median in-hospital stay was eight days (2–45 days). There were in-hospital complications in 16.5%, urinary tract infections, and pneumonia being the most frequent. One-year mortality was 16.7%. Reference values for the normal population of the same age are 5.9% for men and 4.0% for women. One-year mortality rate was 22.2% in the patient group of 80 years or above and 8.8% in the patient group below the age of 80. The rate of surviving patients living at home with or without assistance dropped from 80.5% to 65.3%. The median EQ-5D-Index Value was 0.62 (0.04–1; IQR 0.5–0.78). Reference value for the normal population is 0.78. Average PMS was 4 and NRS 3. Within a two-year period, additional fragility fractures occurred in 21.2% and antiresorptive medication was taken by only 45.2% of patients. Conclusion. Pubic ramus fractures without involvement of the posterior pelvis (FFP Type I) are serious adverse events for elderly persons. During follow-up, there is an excess mortality, a loss of independence, a restricted mobility, and a decreased quality of life. Pubic ramus fractures are indicators for the need to optimize the patient’s general condition.
Correlation Between Tumor-Associated Macrophage and Immune Checkpoint Molecule Expression and Its Prognostic Significance in Cutaneous Melanoma
Authors: Kim Young Jae , Won Chong Hyun , Lee Mi Woo , Choi Jee Ho , Chang Sung Eun , Lee Woo Jin
Published: August 2020

The association between tumor-associated macrophages (TAMs) and the expression of immune checkpoint molecules has not been well described in cutaneous melanoma. We evaluated the correlations between the expression of markers of TAMs, cluster of differentiation 163 (CD163), and immune checkpoint molecules, programmed cell death protein-1 (PD-1), and lymphocyte activating gene-3 (LAG-3). We also determined their relationships with the clinicopathological features and disease outcomes in melanoma. Diagnostic tissues collected from melanoma patients were evaluated using immunohistochemistry for CD163, PD-1, and LAG-3 expression. CD163 expression positively correlated with PD-1 and LAG-3 expression. High expression of both CD163 and PD-1 expressions was significantly associated with negative prognostic factors and worse prognosis than high expression of the single markers. High co-expression of CD163 and LAG-3 was associated with poor clinicopathological indexes of melanoma and worse survival compared to the high expression of the single markers. The expression of immune checkpoint molecules PD-1 and LAG-3 positively correlated with the M2-TAM density in melanoma tissue. Simultaneous high M2-TAM density and immune checkpoint molecules expression acted as independent poor prognostic factors in cutaneous melanoma.
Utility of Minimally Invasive Technology for Inguinal Lymph Node Dissection in Penile Cancer
Authors: Nabavizadeh Reza , Petrinec Benjamin , Necchi Andrea , Tsaur Igor , Albersen Maarten , Master Viraj
Published: August 2020

Our aim is to review the benefits as well as techniques, surgical outcomes, and complications of minimally invasive inguinal lymph node dissection (ILND) for penile cancer. The PubMed, Wiley Online Library, and Science Direct databases were reviewed in March 2020 for relevant studies limited to those published in English and within 2000–2020. Thirty-one articles describing minimally invasive ILND were identified for review. ILND has an important role in both staging and treatment of penile cancer. Minimally invasive technologies have been utilized to perform ILND in penile cancer patients with non-palpable inguinal lymph nodes and intermediate to high-risk primary tumors or patients with unilateral palpable non-fixed inguinal lymph nodes measuring less than 4 cm, including videoscopic endoscopic inguinal lymphadenectomy (VEIL) and robotic videoscopic endoscopic inguinal lymphadenectomy (RVEIL). Current data suggest that VEIL and RVEIL are feasible and safe with minimal intra-operative complications. Perhaps the strongest appeal for the use of minimally-invasive approaches is their faster post-operative recovery and less post-operative complications. As a result, patients can tolerate this procedure better and surgeons can offer surgery to patients who otherwise would not be a candidate or personally willing to undergo surgery. When compared to open technique, VEIL and RVEIL have similar dissected nodal count, a surrogate metric for oncological adequacy, and a none-inferior inguinal recurrence rate. Larger randomized studies are encouraged to investigate long-term outcome and survival rates using these minimally-invasive techniques for ILND.
EEG is A Predictor of Neuroimaging Abnormalities in Pediatric Extracorporeal Membrane Oxygenation
Authors: Fox Jordana , Jenks Christopher L. , Farhat Abdelaziz , Li Xilong , Liu Yulun , James Ellen , Karasick Stephanie , Morriss Michael C. , Sirsi Deepa , Raman Lakshmi
Published: August 2020

The goal of this project was to evaluate if severity of electroencephalogram (EEG) during or shortly after being placed on extracorporeal membrane oxygenation (ECMO) would correlate with neuroimaging abnormalities, and if that could be used as an early indicator of neurologic injury. This was a retrospective chart review spanning November 2009 to May 2018. Patients who had an EEG recording during ECMO or within 48 hours after being decannulated (early group) or within 3 months of being on ECMO (late group) were included if they also had ECMO-related neuroimaging. In the early EEG group, severity of the EEG findings of mild, moderate, and severe EEG correlated to mild, moderate, and severe neuroimaging scores. Patients on venoarterial (VA) ECMO were noted to have higher EEG and neuroimaging severity; this was statistically significant. There was no association in the late EEG group to neuroimaging abnormalities. Our study highlights that EEG severity can be an early predictor for neuroimaging abnormalities that can be identified by computed tomography (CT) and or magnetic resonance imaging (MRI). This can provide guidance for both the medical team and families, allowing for a better understanding of overall prognosis.
Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers
Authors: Hardy Michael , Lessire Sarah , Kasikci Sultan , Baudar Justine , Guldenpfennig Maité , Collard Adrien , Dogné Jean-Michel , Chatelain Bernard , Jacqmin Hugues , Lecompte Thomas , Mullier François
Published: August 2020

Platelet count, indices (mean volume, young—immature platelet fraction) and aggregation are widely used laboratory parameters to investigate primary hemostasis. We performed a systematic, thorough evaluation of the influence of the time-interval since blood draw from 20 healthy individuals and of the anticoagulation of collected blood on such parameters. Blood was anticoagulated with citrate, K2-ethylenediaminetetraacetic acid (EDTA) and hirudin and analyzed 5, 30, 60, 120 and 180 min after blood draw. Multiple electrode aggregometry (MEA) was performed with either hirudin (half-diluted with NaCl) or citrate samples (half-diluted with NaCl or CaCl2 3 mM). Platelet count and indices (Sysmex XN-20) were rather stable over time with EDTA blood. MEA results were lower with citrate blood than with hirudin blood; supplementation with calcium was partially compensatory. MEA results were also lower when performed less than 30 or more than 120 min after blood draw. Platelet clumping, quantitatively estimated with microscope examination of blood smears, was more important in hirudin blood than citrate or EDTA blood and could explain some of the differences observed between preanalytical variables. The results stress once more the importance of preanalytical variables in hemostasis laboratory testing. Decision thresholds based on those tests are only applicable within specific preanalytical conditions.
Health and Safety Concerns Related to CNT and Graphene Products, and Related Composites
Authors: Sousa Susana P.B. , Peixoto Tânia , Santos Raquel M. , Lopes Ascensão , Paiva Maria da Conceição , Marques António T.
Published: August 2020

The use of Carbon Nanotubes (CNT) and Graphene increased in the last decade and it is likely to keep increasing in the near future. The attractiveness of their properties, particularly the possibility to enhance the composites performance using a tailor made methodology, brings new materials, processes and products for highly demanding industrial applications and to the market. However, there are quite a lot of health/safety issues, as well as lack of understanding and standards to evaluate their effects. This paper starts with a general description of materials, processes and products dealing with CNT and graphene. Then, an overview of concerns related to the health and safety when handling, researching, producing and using products that include these materials is presented. It follows a risk management approach with respect to simulation and evaluation tools, and considering the consensual limits already existing for research, industry and consumers. A general discussion integrating the relevant aspects of health and safety with respect to CNT and graphene is also presented. A proactive view is presented with the intention to contribute with some guidelines on installation, maintenance, evaluation, personal protection equipment (PPE) and personnel training to deal with these carbon-based nanomaterials in research, manufacture, and use with composite materials.
Influence of Superhydrophobic Coating on the Water Resistance of Foundry Dust/Magnesium Oxychloride Cement Composite
Authors: Wang Fajun , Zhu Xiantao , Liu Huangjuan , Lei Sheng , Huang Daqi
Published: August 2020

In this work, magnesium oxychloride cement (MOC) was used to realize the resource use of foundry dust (FD). Portland cement (PC)-based superhydrophobic coating was prepared on the surface of FD/MOC composite to improve the water resistance of the composite. First, the FD/MOC composites with different contents of FD were prepared. The phase structure of the composite was analyzed using X-ray diffraction (XRD). The microstructure of the cross-section and surface of the composite was observed using field emission scanning electron microscope (FE-SEM). The mechanical properties of the FD/MOC composites with different FD contents at different ages were tested and analyzed. Secondly, the superhydrophobic coating was prepared on the surface of MOC composite using silane/siloxane aqueous emulsion as the hydrophobic modifier, PC as the matrix and water as the solvent. The microstructure and chemical composition of the PC-based superhydrophobic coating were tested and analyzed. The results show that FD can significantly improve the early strength of the FD/MOC composite. The 28-day compressive strength of the FD/MOC composite decreases with increasing FD content. When the FD content is 30%, the 28-day compressive strength of the FD/MOC composite is as high as 75.68 MPa. Superhydrophobic coating can effectively improve the water resistance of the FD/MOC composite. The softening coefficient of the FD/MOC composite without superhydrophobic coating is less than 0.26, while that of the composite modified by superhydrophobic coating is greater than 0.81.
Progress of Bromophenols in Marine Algae from 2011 to 2020: Structure, Bioactivities, and Applications
Authors: Dong Hui , Dong Songtao , Erik Hansen Poul , Stagos Dimitrios , Lin Xiukun , Liu Ming
Published: August 2020

Marine algae contain various bromophenols that have been shown to possess a variety of biological activities, including antiradical, antimicrobial, anticancer, antidiabetic, anti-inflammatory effects, and so on. Here, we briefly review the recent progress of these marine algae biomaterials and their derivatives from 2011 to 2020, with respect to structure, bioactivities, and their potential application as pharmaceuticals.
Fucoidan Supplementation Restores Fecal Lysozyme Concentrations in High-Performance Athletes: A Pilot Study
Authors: Cox Amanda J. , Cripps Allan W. , Taylor Phillipa A. , Fitton J. Helen , West Nicholas P.
Published: August 2020

Nutritional strategies to help promote immune competence are of particular interest for a range of population groups. This study aimed to assess the potential impacts of fucoidan, a seaweed-derived bioactive polysaccharide, on gut markers of immunity and inflammation. A group of professional team-sport athletes were selected for inclusion in the study given the recognized potential for intense physical activity to induce alterations in immune function. A retrospective analysis was performed on stored fecal samples which had been collected from professional team-sport athletes (n = 22) and healthy adults (n = 11) before and after seven days of supplementation with fucoidan (Fucus vesiculosus/Undaria pinnatifida extract, 1 g/d). Fecal concentrations of calprotectin, secretory immunoglobulin A (sIgA) and lysozyme were determined using enzyme-linked immunosorbent assays. The supplement was well tolerated by participants with no adverse events reported. At baseline, fecal lysozyme concentrations were ~73% higher in the healthy adults compared to the professional athletes (p = 0.001). For the professional athletes, a significant (~45%) increase in fecal lysozyme was observed following the supplementation period (p = 0.001). These data suggest that fucoidan supplementation may have the potential to promote the secretion of antimicrobial peptides in specific population groups and contribute to the regulation of mucosal immune health.
Production of a Phosphate Concentrate from the Tailings of a Niobium Ore Concentrator
Authors: Clapperton Anthony , Bazin Claude , Downey Dominic , Marois Jean-Sébastien
Published: August 2020

Apatite is the main source of phosphorous for the making of chemical fertilizers. While apatite is usually recovered from phosphate orebodies as the primary product of a mining exploitation, this paper documents the approach taken to produce a phosphate concentrate as a secondary product from the tailings of a niobium ore concentrator. The conventional desliming/flotation scheme used to process phosphate ores was tested and adapted to process one of the reject streams of a niobium concentrator in order to produce a salable phosphate concentrate. For that particular material, it was found that the reverse flotation of apatite yielded better results than the commonly used direct flotation of apatite. The recommended approach to produce the phosphate concentrate is desliming followed by reverse flotation of apatite and an acid leaching of the apatite concentrate to lower the MgO content below the specification for a phosphate concentrate. The obtained phosphate concentrate assays more than 32% P2O5 at a P2O5 recovery of 41%, which although low is found to be economic for the case of processing plant reject tailings.