bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Evaluation on the Bioaccessibility of Citrus Fruit Carotenoids In Vitro Based on Different Intake Patterns
Authors: Xu Yang , Hu Tan , Hu Haijuan , Xiong Sihui , Shi Kaixin , Zhang Nawei , Mu Qier , Xu Gang , Zhang Peipei , Pan Siyi
Published: May 2022

The intake pattern has a great impact on the bioaccessibility of carotenoids from citrus fruit. Here, we compared the bioaccessibility of carotenoids from fresh citrus fruit (FC), fresh citrus juice (FCJ), and not-from-concentrate citrus juice (NCJ) and analyzed the influencing factors. The results demonstrated that particle size, viscosity, and some active components of the samples during digestion are potential factors affecting the bioaccessibility of carotenoids. The total carotenoid bioaccessibility of NCJ (31.45 ± 2.58%) was significantly higher than that of FC (8.11 ± 0.43%) and FCJ (12.43 ± 0.49%). This work demonstrates that NCJ is an appropriate intake pattern to improve the bioaccessibility of carotenoids from citrus fruit. The findings also suggest that adjustment of food intake patterns is an effective way to improve the digestion and absorption of nutrients.
Development of Functional Pizza Base Enriched with Jujube (Ziziphus jujuba) Powder
Authors: Falciano Aniello , Sorrentino Angela , Masi Paolo , Di Pierro Prospero
Published: May 2022

Functional and enriched foods are increasingly in demand in the global market due to their benefits for human health and their prevention of several diseases. The aim of this work was to develop a functional pizza base, produced in the Neapolitan style, exploiting the beneficial properties of jujube. The jujube fruit is rich in phenolic compounds with high antioxidant activity and represents a good candidate for functional food development. The doughs were prepared by replacing the wheat flour with 2.5%, 5.0%, and 7.5% (w/w) of Ziziphus jujube powder (ZJP) and were subsequently cooked. Chemical analyses showed that both total phenolic compounds and antioxidant activity grew with the increase of ZJP. The addition of ZJP darkened the pizza base and raised its hardness, gumminess, and chewiness. However, no difference was found in the springiness and cohesiveness of the samples with or without ZJP. These results suggest that jujube powder can be successfully introduced into pizza dough as a functional ingredient.
Prevalence and Persistence of Multidrug-Resistant Yersinia enterocolitica 4/O:3 in Tonsils of Slaughter Pigs from Different Housing Systems in Croatia
Authors: Zdolec Nevijo , Kiš Marta , Jankuloski Dean , Blagoevska Katerina , Kazazić Snježana , Pavlak Marina , Blagojević Bojan , Antić Dragan , Fredriksson-Ahomaa Maria , Pažin Valerij
Published: May 2022

Yersinia enterocolitica is one of the priority biological hazards in pork inspection. Persistence of the pathogen, including strains resistant to antimicrobials, should be evaluated in pigs from different housing systems for risk ranking of farms. In this 2019 study, tonsils were collected from 234 pigs, of which 69 (29.5%) were fattened on 3 big integrated farms, 130 (55.5%) on 10 medium-sized farms, and 35 (15%) on 13 small family farms. In addition, 92 pork cuts and minced meat samples from the same farms were tested for the presence of Y. enterocolitica using the culture method. Phenotypic and genetic characteristics of the isolates were compared with previously collected isolates from 2014. The overall prevalence of Y. enterocolitica in pig tonsils was 43% [95% CI 36.7–49.7]. In pigs from big integrated, medium-sized, and small family farms, the prevalence was 29%, 52%, and 40%, respectively. All retail samples of portioned and minced pork tested negative for pathogenic Y. enterocolitica, likely due to high hygienic standards in slaughterhouses/cutting meat or low sensitivity of culture methods in these matrices. The highest recovery rate of the pathogen from tonsils was found when alkali-treated PSB and CIN agar were combined. The biosecurity category of integrated and medium farms did not affect the differences in prevalence of Y. enterocolitica (p > 0.05), in contrast to family farms. Pathogenic ail-positive Y. enterocolitica biotype 4 serotype O:3 persisted in the tonsils of pigs regardless of the type of farm, slaughterhouse, and year of isolation 2014 and 2019. PFGE typing revealed the high genetic concordance (80.6 to 100%) of all the Y. enterocolitica 4/O:3 isolates. A statistically significant higher prevalence of multidrug-resistant Y. enterocolitica 4/O:3 isolates was detected in the tonsils of pigs from big integrated farms compared to the other farm types (p < 0.05), with predominant and increasing resistance to nalidixic acid, chloramphenicol, and streptomycin. This study demonstrated multidrug resistance of the pathogen in pigs likely due to more antimicrobial pressure on big farms, with intriguing resistance to some clinically relevant antimicrobials used in the treatment of yersiniosis in humans.
Sprouts Use as Functional Foods. Optimization of Germination of Wheat (Triticum aestivum L.), Alfalfa (Medicago sativa L.), and Radish (Raphanus sativus L.) Seeds Based on Their Nutritional Content Evolution
Authors: Francis Helga , Debs Espérance , Koubaa Mohamed , Alrayess Zeina , Maroun Richard G. , Louka Nicolas
Published: May 2022

Wheat, alfalfa, and radish sprouts are well-renowned for their high nutritional content. However, their optimal imbibition and germination durations are rarely considered in the literature. In this study, reduced imbibition times of 3 h, 10 h, and 4 h were demonstrated for the wheat, alfalfa, and radish seeds, respectively. The evolution of their crude fat, proteins, polyphenols, antioxidant activity, and vitamins were investigated over 7 days of germination. The crude fat and protein loads of these sprouts slightly varied during germination, whereas the phenolic compounds and antioxidant activity maxed out at day 7, 5, and 6 for the wheat, alfalfa, and radish sprouts, respectively, with significant levels of catechin. The vitamins highly increased, showing noteworthy yet different peaks of growth depending on the seed and the vitamin analyzed. Interestingly, alfalfa and radish sprouts, taken at their optimal germination day, would decidedly contribute to meet our Recommended Daily Allowances (RDAs) of vitamins E, A, and B6. Overall, for a greater nutritional content and a potential use of these sprouts as nutraceutical ingredients, our results suggested to leave the wheat, alfalfa, and radish seeds to germinate only over 7, 4, and 6 days, respectively, after which their nutritional quality tended to decrease.
The Potential of Phaeodactylum as a Natural Source of Antioxidants for Fish Oil Stabilization
Authors: Demets Robbe , Van Broekhoven Simon , Gheysen Lore , Van Loey Ann , Foubert Imogen
Published: May 2022

Worldwide, fish oil is an important and rich source of the health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). It is, however, troubled by its high susceptibility towards lipid oxidation. This can be prevented by the addition of (preferably natural) antioxidants. The current research investigates the potential of Phaeodactylum carotenoids in this regard. The oxidative stability of fish oil and fish oil with Phaeodactylum addition is evaluated by analyzing both primary (PV) and secondary (volatiles) oxidation products in an accelerated storage experiment (37 °C). A first experimental set-up shows that the addition of 2.5% (w/w) Phaeodactylum biomass is not capable of inhibiting oxidation. Although carotenoids from the Phaeodactylum biomass are measured in the fish oil phase, their presence does not suffice. In a second, more elucidating experimental set-up, fish oil is mixed in different proportions with a Phaeodactylum total lipid extract, and oxidative stability is again evaluated. It was shown that the amount of carotenoids relative to the n-3 LC-PUFA content determined oxidative stability. Systems with a fucoxanthin/n-3 LC-PUFA ratio ≥ 0.101 shows extreme oxidative stability, while systems with a fucoxanthin/n-3 LC-PUFA ratio ≤ 0.0078 are extremely oxidatively unstable. This explains why the Phaeodactylum biomass addition did not induce oxidative stability.
Novel Thermostable Heparinase Based on the Genome of Bacteroides Isolated from Human Gut Microbiota
Authors: Zhang Chuan , Yu Leilei , Zhai Qixiao , Zhao Ruohan , Wang Chen , Zhao Jianxin , Zhang Hao , Chen Wei , Tian Fengwei
Published: May 2022

Among the nutrients available to the human gut microbiota, the complex carbohydrates and glycosaminoglycans are important sources of carbon for some of the species of human gut microbiota. Glycosaminoglycan (heparin) from the host is a highly preferred carbohydrate for Bacteroides. To explore how gut microbiota can effectively use heparin as a carbon source for growth, we conducted a screening of the Carbohydrate-Active enzymes (CAZymes) database for lytic enzymes of the PL13 family and Research Center of Food Biotechnology at School of Food Science and Technology of Jiangnan University database of Bacteroides to identify novel glycosaminoglycan-degrading bacterial strains. Four Bacteroides species (Bacteroides eggerthii, Bacteroides clarus, Bacteroides nordii, and Bacteroides finegoldii) that degraded heparin were selected for further studies. Analysis of the polysaccharide utilization sites of the four strains revealed that all of them harbored enzyme encoding genes of the PL13 family. Functional analysis revealed the activity of CAZymes in a medium containing heparin as the sole carbon source, suggesting their potential to degrade heparin and support growth. The four enzymes were heterologous expressed, and their enzymatic properties, kinetics, and thermal stability were determined. The lytic enzyme of B. nordii had high enzymatic activity and thermal stability. The features that cause this high thermal stability were elucidated based on an examination of the three-dimensional structure of the protein. Our findings provide an important theoretical basis for the application of glycosaminoglycans and glycosaminoglycan-degrading enzymes in the medical and biotechnology industries, and an important scientific basis for precision nutrition and medical intervention studies using gut microbiota or enzymes as targets.
Boiling, Blanching, and Stir-Frying Markedly Reduce Pesticide Residues in Vegetables
Authors: Phopin Kamonrat , Wanwimolruk Sompon , Norkaew Chosita , Buddhaprom Jaruwat , Isarankura-Na-Ayudhya Chartchalerm
Published: May 2022

Nowadays, a lot of produce (fruits and vegetables) sold in many countries are contaminated with pesticide residues, which cause severe effects on consumer health, such as cancer and neurological disorders. Therefore, this study aims to determine whether cooking processes can reduce the pesticide residues in commonly consumed vegetables (Chinese kale and yard long beans) in Thailand. For cooking experiments, the two vegetables were cooked using three different processes: boiling, blanching, and stir-frying. After the treatments, all cooked and control samples were subjected to extraction and GC-MS/MS analysis for 88 pesticides. The results demonstrated that pesticide residues were reduced by 18–71% after boiling, 36–100% after blanching, and 25–60% after stir-frying for Chinese kale. For yard long beans, pesticide residues were reduced by 38–100% after boiling, 27–28% after blanching, and 35–63% after stir-frying. Therefore, cooking vegetables are proven to protect consumers from ingesting pesticide residues.
Anti-Diabetic Activity of Polysaccharides from Auricularia cornea var. Li.
Authors: Fu Yuan , Wang Liwen , Jiang Guochuan , Ren Lili , Wang Liyan , Liu Xuejun
Published: May 2022

Auricularia cornea var. Li. polysaccharide (ACP) has many important biological activities and has potential application value in food engineering, pharmaceutical science, and health care. The results were as follows: the extraction rate of ACP was 28.18% ± 1.41% and the purity of ACP was 86.92% ± 2.80%. ACP contains mannitol 32.41%, glucuronic acid 6.96%, rhamnose 0.32%, glucose 42.35%, galactose 0.77%, xylose 16.83%, and fucose 0.36%, without galacturonic acid and arabinose. In addition, the results of an animal test of diabetes mellitus II (DM II) with ACP showed that the total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and fasting blood glucose and water in the serum of mice with ACP were significantly lower than those in the model group; the serum SOD, hepatic glycogen, and insulin of mice added with ACP were significantly higher than those in the model group. More importantly, ACP had no significant adverse effects on organ index and liver and kidney tissue morphology in mice. These results suggest that ACP can be used as a potential functional food component for the prevention or treatment of diabetes.
Miniaturized NIR Spectroscopy in Food Analysis and Quality Control: Promises, Challenges, and Perspectives
Authors: Beć Krzysztof B. , Grabska Justyna , Huck Christian W.
Published: May 2022

The ongoing miniaturization of spectrometers creates a perfect synergy with the common advantages of near-infrared (NIR) spectroscopy, which together provide particularly significant benefits in the field of food analysis. The combination of portability and direct onsite application with high throughput and a noninvasive way of analysis is a decisive advantage in the food industry, which features a diverse production and supply chain. A miniaturized NIR analytical framework is readily applicable to combat various food safety risks, where compromised quality may result from an accidental or intentional (i.e., food fraud) origin. In this review, the characteristics of miniaturized NIR sensors are discussed in comparison to benchtop laboratory spectrometers regarding their performance, applicability, and optimization of methodology. Miniaturized NIR spectrometers remarkably increase the flexibility of analysis; however, various factors affect the performance of these devices in different analytical scenarios. Currently, it is a focused research direction to perform systematic evaluation studies of the accuracy and reliability of various miniaturized spectrometers that are based on different technologies; e.g., Fourier transform (FT)-NIR, micro-optoelectro-mechanical system (MOEMS)-based Hadamard mask, or linear variable filter (LVF) coupled with an array detector, among others. Progressing technology has been accompanied by innovative data-analysis methods integrated into the package of a micro-NIR analytical framework to improve its accuracy, reliability, and applicability. Advanced calibration methods (e.g., artificial neural networks (ANN) and nonlinear regression) directly improve the performance of miniaturized instruments in challenging analyses, and balance the accuracy of these instruments toward laboratory spectrometers. The quantum-mechanical simulation of NIR spectra reveals the wavenumber regions where the best-correlated spectral information resides and unveils the interactions of the target analyte with the surrounding matrix, ultimately enhancing the information gathered from the NIR spectra. A data-fusion framework offers a combination of spectral information from sensors that operate in different wavelength regions and enables parallelization of spectral pretreatments. This set of methods enables the intelligent design of future NIR analyses using miniaturized instruments, which is critically important for samples with a complex matrix typical of food raw material and shelf products.
Saponification Value of Fats and Oils as Determined from 1H-NMR Data: The Case of Dairy Fats
Authors: Ivanova Mihaela , Hanganu Anamaria , Dumitriu Raluca , Tociu Mihaela , Ivanov Galin , Stavarache Cristina , Popescu Liliana , Ghendov-Mosanu Aliona , Sturza Rodica , Deleanu Calin , Chira Nicoleta-Aurelia
Published: May 2022

The saponification value of fats and oils is one of the most common quality indices, reflecting the mean molecular weight of the constituting triacylglycerols. Proton nuclear magnetic resonance (1H-NMR) spectra of fats and oils display specific resonances for the protons from the structural patterns of the triacylglycerols (i.e., the glycerol backbone), methylene (-CH2-) groups, double bonds (-CH=CH-) and the terminal methyl (-CH3) group from the three fatty acyl chains. Consequently, chemometric equations based on the integral values of the 1H-NMR resonances allow for the calculation of the mean molecular weight of triacylglycerol species, leading to the determination of the number of moles of triacylglycerol species per 1 g of fat and eventually to the calculation of the saponification value (SV), expressed as mg KOH/g of fat. The algorithm was verified on a series of binary mixtures of tributyrin (TB) and vegetable oils (i.e., soybean and rapeseed oils) in various ratios, ensuring a wide range of SV. Compared to the conventional technique for SV determination (ISO 3657:2013) based on titration, the obtained 1H-NMR-based saponification values differed by a mean percent deviation of 3%, suggesting the new method is a convenient and rapid alternate approach. Moreover, compared to other reported methods of determining the SV from spectroscopic data, this method is not based on regression equations and, consequently, does not require calibration from a database, as the SV is computed directly and independently from the 1H-NMR spectrum of a given oil/fat sample.
Characterizing the Structural Evolution of Cereal Trade Networks in the Belt and Road Regions: A Network Analysis Approach
Authors: Chen Wei , Zhang Haipeng
Published: May 2022

Cereal trade is essential for economic and commercial cooperation among countries along the “Belt and Road” (BRI). It helps ensure food security and contributes to building a community of interests and destinies for the BRI countries. Based on the UN Comtrade database, this study, using a network analysis approach, investigates the structural characteristics and spatiotemporal dynamics of cereal trade networks among the “Belt and Road” countries. Results show that: (1) The cereal trade among the BRI countries has formed well-connected and complex trade networks, and the “Belt and Road” initiative has significantly promoted cereal trade networks among the BRI countries. (2) The backbone structures of cereal trade networks along the BRI are in geographical proximity. India, Russia, and Ukraine are the most important trading partners and absolute core nodes in the trade networks, influencing the entire cereal trade networks. (3) The BRI cereal trade networks exhibit significant core-periphery structures, with considerable power asymmetries between the countries reflecting food supply and demand differences. In general, the BRI cereal trade networks have developed from relatively diversified to polarized. Supply chains in the cereal trade network are dominated by a few large countries and are fragile, with weak resilience and low resistance to risk. Therefore, governments should continue to strengthen regional cooperation, optimize cereal trade network structure, enhance their reserve capacity, and build a stronger system to guarantee food security and prevent risk. All these measures will support the food security of the “Belt and Road” countries.
Curcumin-Loaded Liposome Preparation in Ultrasound Environment under Pressurized Carbon Dioxide
Authors: Wahyudiono  , He Jiayang , Hu Xin , Machmudah Siti , Yasuda Keiji , Takami Seiichi , Kanda Hideki , Goto Motonobu
Published: May 2022

Curcumin-loaded liposomes were prepared using a supercritical carbon dioxide (SCCO2)–ultrasound environment system. The experiments were performed at temperatures of 40–70 °C and pressures of 10–25 MPa in a batch system with ultrasonication for 60 min. Transmission electron microscopy (TEM) images revealed liposome products with spherical morphologies and diameters of <100 nm. Dynamic light scattering (DLS) analysis indicated that the curcumin-loaded liposome nanosuspension exhibited good stability. Changing the operating conditions influenced the amount of liposome-encapsulated curcumin; as the operating temperature or pressure increased, the diameter of the liposome products and the amount of liposome-encapsulated curcumin increased and decreased, respectively. Herein, we described an innovative and practical organic-solvent-free method for generating liposomes from phospholipids.
A Mass Dependent Density Profile from Dwarfs to Clusters
Authors: Del Popolo Antonino , Le Delliou Morgan
Published: May 2022

In this paper, we extend the work of Freundlich et al. 2020 who showed how to obtain a Dekel–Zhao density profile with mass dependent shape parameters in the case of galaxies. In the case of Freundlich et al. 2020, the baryonic dependence was obtained using the NIHAO set of simulations. In our case, we used simulations based on a model of ours. Following Freundlich et al. 2020, we obtained the dependence from baryon physics of the two shape parameters, obtaining in this way a mass dependent Dekel–Zhao profile describing the dark matter profiles from galaxies to clusters of galaxies. The extension to the Dekel–Zhao mass dependent profile to clusters of galaxies is the main result of the paper. In the paper, we show how the Dekel–Zhao mass dependent profile gives a good description of the density profiles of galaxies, already shown by Freundlich et al. 2020, but also to a set of clusters of galaxies.
Do Red Seaweed Nanoparticles Enhance Bioremediation Capacity of Toxic Dyes from Aqueous Solution?
Authors: Mansour Abdallah Tageldein , Alprol Ahmed E. , Ashour Mohamed , Ramadan Khaled M. A. , Alhajji Adnan H. M. , Abualnaja Khamael M.
Published: May 2022

Based on their functional groups, the use of various seaweed forms in phytoremediation has recently gained significant eco-friendly importance. The objective of this study was to determine whether a novel, sustainable, and ecologically acceptable adsorbent could be employed to remove toxic textile dye (Ismate Violet 2R (IV2R)) from an aqueous solution. The low-cost adsorbent was prepared from the nanoparticles form of the native red seaweed species, Pterocladia capillacea. Before and after the adsorption procedure, comprehensive characterization experiments on the bio-adsorbent were carried out, including BET, SEM, FTIR, UV, and dynamic light scattering (DLS) examination. The adsorption performance of the prepared nano-Pterocladia capillacea was optimized by adjusting operating parameters such as the initial dye concentration of 60 mg L−1, pH of 2, and contact time of 15 min, all of which were obtained by batch experiments in the lab. At the optimum conditions, the prepared adsorbent had maximum removal effectiveness of 87.2%. Most typical kinetics and isotherm models were used to test the experimental results. The equilibrium data fit well with the Langmuir isotherm model, with comparatively higher R2 values and fewer standard errors, while the pseudo-second-order kinetic model fits better with a decent correlation coefficient. Thermodynamic parameters revealed that the sorption process on nano-alga was exothermic and spontaneous.
Effectiveness of Fucoidan on Supplemental Therapy in Cancer Patients: A Systematic Review
Authors: Wu Chih-Jung , Yeh Tzu-Pei , Wang Ya-Jung , Hu Hsiao-Fang , Tsay Shiow-Luan , Liu Liang-Chih
Published: May 2022

Purpose: Fucoidan is a dietary supplement which is commonly used by cancer patients. However, despite evidence of positive effects in cell culture environments, there are currently no clinical guidelines for supplementary use of fucoidan in cancer patients. This study aims to evaluate the effectiveness of fucoidan supplemental use. Methods: A systematic literature search was conducted using databases including Cochrane Library, JBI, PubMed, Embase, and CINAHL. All original studies on fucoidan for supplemental use in cancer patients were included. The search was made in databases without time restriction. The outcomes included disease progression status, inflammatory markers, nutritional status, adverse effects, and quality of life. The appraisal tool used was JBI-MAStARI. Results: Four studies were included: One randomized controlled trial and three quasi-experimental studies. Meta-analysis was not applied due to the heterogeneity of measurement tools. Overall sample size was 118. Most participants were metastatic colorectal and gastric cancer patients. Two studies revealed a significantly longer survival time and chemotherapy treatment periods with fucoidan use. Positive but insignificant effects of disease control rate, inflammatory markers, nutrition status, fatigue, and financial difficulty were shown in those using fucoidan. Conclusions: The results of this systematic review indicate that the effects of fucoidan were inconsistent with clinical outcomes in metastatic or recurrent cancer patients. Only four studies were included, and heterogeneity in methodologies and relatively small sample sizes limited the research consensus. Although cause and effect between fucoidan and the survival time, disease control or adverse effects could not be confirmed, this study includes the most research on fucoidan in humans.
Creating Coherence-Based Nurse Planning in the Perinatology Care System
Authors: Winasti Windi , Elkhuizen Sylvia G. , van Merode Frits , Berden Hubert
Published: May 2022

The combination of increasing demand and a shortage of nurses puts pressure on hospital care systems to use their current volume of resources more efficiently and effectively. This study focused on gaining insight into how nurses can be assigned to units in a perinatology care system to balance patient demand with the available nurses. Discrete event simulation was used to evaluate the what-if analysis of nurse flexibility strategies and care system configurations from a case study of the Perinatology Care System at Radboud University Medical Center in Nijmegen, the Netherlands. Decisions to exercise nurse flexibility strategies to solve supply–demand mismatches were made by considering the entire patient care trajectory perspective, as they necessitate a coherence perspective (i.e., taking the interdependency between departments into account). The study results showed that in the current care system configuration, where care is delivered in six independent units, implementing a nurse flexibility strategy based on skill requirements was the best solution, averaging two fewer under-/overstaffed nurses per shift in the care system. However, exercising flexibility below or above a certain limit did not substantially improve the performance of the system. To meet the actual demand in the studied setting (70 beds), the ideal range of flexibility was between 7% and 20% of scheduled nurses per shift. When the care system was configured differently (i.e., into two large departments or pooling units into one large department), supply–demand mismatches were also minimized without having to implement any of the three nurse flexibility strategies mentioned in this study. These results provide insights into the possible solutions that can be implemented to deal with nurse shortages, given that these shortages could potentially worsen in the coming years.
Does Self-Directed Learning with Simulation Improve Critical Thinking and Motivation of Nursing Students? A Pre-Post Intervention Study with the MAES© Methodology
Authors: Arizo-Luque Vanessa , Ramirez-Baena Lucía , Pujalte-Jesús María José , Rodríguez-Herrera María Ángeles , Lozano-Molina Ainhoa , Arrogante Oscar , Díaz-Agea José Luis
Published: May 2022

Motivation and critical thinking are fundamental for the development of adequate learning. The purpose of the present study was to assess the motivation for learning and critical thinking among nursing students before and after self-directed simulation-based training using the MAES© methodology. A cross-sectional and descriptive quantitative study was conducted with a sample of third-year nursing students. The instruments utilized were the Spanish-adapted version of the Motivated Strategies for Learning Questionnaire (MSLQ-44), and the Critical Thinking for Nursing Professionals Questionnaire (CuPCPE). The students improved their levels both of motivation components, (such as self-efficacy, strategy use, self-regulation) and critical thinking components (such as personal characteristics, intellectual and cognitive abilities, interpersonal abilities and self-management, and technical abilities). These improvements could be a result of the intrinsic characteristics of the MAES© methodology (as a team-based, self-directed, collaborative and peer-to-peer learning method).
What Topics Do Members of the Eating Disorder Online Community Discuss and Empathize with? An Application of Big Data Analytics
Authors: Park Eunhye , Kim Woo-Hyuk , Kim Sung-Bum
Published: May 2022

Given the stigma of eating disorders (EDs), anonymous online communities of individuals with EDs can play a critical role in their treatment. In our study, we aimed to identify prevalent topics related to EDs in one community. To discover latent topics in an online community dedicated to EDs, we applied an automated text-mining approach to topic modeling after collecting data from Reddit created between January 2011 and September 2020. As a result of topic modeling, topics regarding emotional support, informational support, and inquiries about EDs were discovered. In frequently asked questions and advice about EDs in the online community, community members empathized more with topics soliciting emotional support rather than informational support. Despite the importance of online communities, studies on this topic, especially those analyzing online conversations, have remained limited. By demonstrating the role of online communities in supplementary interventions, our findings can support clinicians in caring for patients with EDs.
Evidence-Based Data Models for Return-to-Play Criteria after Anterior Cruciate Ligament Reconstruction
Authors: Daggett Matthew C. , Witte Kevin A. , Cabarkapa Dimitrije , Cabarkapa Damjana V. , Fry Andrew C.
Published: May 2022

The anterior cruciate ligament (ACL) tear is one of the most common knee injuries in sports that require side-to-side pivoting movements. While the timeline and specific goals during rehabilitation protocols may vary, ACL reconstruction (ACLR) is the preferred procedure necessary to return these athletes to their respective field of play. However, there are no validated guidelines that define a specific timepoint when it is safe for an athlete to return-to-play, as functional movement deficit may be present much longer than six months post ACLR. A retrospective cross-sectional analysis was conducted on 33 subjects that underwent ACLR. As a part of standard of care, each subject completed a movement screening protocol at a singular timepoint during their rehabilitation process. An innovative three-dimensional markerless motion capture system was used to obtain three algorithm-derived biometric variables: mobility, alignment, and readiness. Significant gradual improvements in mobility and readiness were observed throughout a 3–6-month post ACLR procedure period. When examining the data trends, it was obvious that not all patients responded identically to treatment plans provided by clinical professionals. Therefore, the findings of the present study suggest that the decision regarding when it is safe to return to play needs to be determined on an individual basis.
Hidden Truth in Cerebral Concussion—Traumatic Axonal Injury: A Narrative Mini-Review
Authors: Jang Sung Ho , Byun Dong Hyun
Published: May 2022

This study reviewed traumatic axonal injury (TAI) in patients with concussion. Concussion refers to transient changes in the neurological function of the brain resulting from head trauma that should not involve any organic brain injury. On the other hand, TAI has been reported in autopsy studies of the human brain and histopathological studies of animal brains following concussion before the development of diffusion tensor imaging (DTI). The diagnosis of TAI in live patients with concussion is limited because of the low resolution of conventional brain magnetic resonance imaging. Since the first study by Arfanakis et al. in 2002, several hundred studies have reported TAI in patients with concussion using DTI. Furthermore, dozens of studies have demonstrated TAI using diffusion tensor tractography for various neural tracts in individual patients with concussion. Hence, DTI provides valuable data for the diagnosis of TAI in patients with concussion. Nevertheless, the confirmation of TAI in live patients with concussion can be limited because a histopathological study via a brain biopsy is required to confirm TAI. Accordingly, further studies for a diagnostic approach to TAI using DTI without a histopathological test in individual patients with concussion will be necessary in the clinical field.
Adaptation of the Highly Sensitive Person Scale (HSP) and Psychometric Properties of Reduced Versions of the Highly Sensitive Person Scale (R-HSP Scale) in Spanish Nursing Students
Authors: Ponce-Valencia Alicia , Jiménez-Rodríguez Diana , Simonelli-Muñoz Agustín Javier , Gallego-Gómez Juana Inés , Castro-Luna Gracia , Echevarría Pérez Paloma
Published: May 2022

Sensory processing sensitivity (SPS) can be defined as a personality characteristic that includes the individual characteristics of sensitivity towards endogenous and exogenous stimuli. The differences in environmental sensitivity can play a crucial role in the academic context of health professionals, thus defining it as an area of research that must be addressed. The reduced scale for highly sensitive people (HSP) is a short (16 items) and adapted version of the original scale for highly sensitive people (HSP). This study aims to analyze the psychometric properties of reduced versions of the Highly Sensitive Person Scale (r-HSP Scale) in Spanish nursing students. Once the questionnaire was translated, its psychometric characteristics were analyzed. The Spanish version of the r-HSP scale was administered to 284 university students enrolled in the Nursing Degree. The results from the factorial analysis confirmed the structure of sensitiveness of six factors in our sample. This structure included the following dimensions: (1) Instability, (2) Surroundings, (3) Interaction with others, (4) Sensoperception, (5) Sensitivity, and (6) Insecurity. Additionally, the Cronbach’s α values indicated that the Spanish version of the r-HSP scale had an adequate reliability (α = 0.702). The r-HSP scale is defined as a reliable, valid, and agile replica of the original structure of sensitivity in Spanish university students.
Disclosing Critical Voice Features for Discriminating between Depression and Insomnia—A Preliminary Study for Developing a Quantitative Method
Authors: Lin Ray F. , Leung Ting-Kai , Liu Yung-Ping , Hu Kai-Rong
Published: May 2022

Background: Depression and insomnia are highly related—insomnia is a common symptom among depression patients, and insomnia can result in depression. Although depression patients and insomnia patients should be treated with different approaches, the lack of practical biological markers makes it difficult to discriminate between depression and insomnia effectively. Purpose: This study aimed to disclose critical vocal features for discriminating between depression and insomnia. Methods: Four groups of patients, comprising six severe-depression patients, four moderate-depression patients, ten insomnia patients, and four patients with chronic pain disorder (CPD) participated in this preliminary study, which aimed to record their speaking voices. An open-source software, openSMILE, was applied to extract 384 voice features. Analysis of variance was used to analyze the effects of the four patient statuses on these voice features. Results: statistical analyses showed significant relationships between patient status and voice features. Patients with severe depression, moderate depression, insomnia, and CPD reacted differently to certain voice features. Critical voice features were reported based on these statistical relationships. Conclusions: This preliminary study shows the potential in developing discriminating models of depression and insomnia using voice features. Future studies should recruit an adequate number of patients to confirm these voice features and increase the number of data for developing a quantitative method.
The Effect of Early Applied Robot-Assisted Physiotherapy on Functional Independence Measure Score in Post-Myocardial Infarction Patients
Authors: Bartík Peter , Vostrý Michal , Hudáková Zuzana , Šagát Peter , Lesňáková Anna , Dukát Andrej
Published: May 2022

Robot-assisted training has been widely used in rehabilitation programs, but no significant clinical evidence about its use in productive working-age cardiac patients was demonstrated. Thus, we hypothesized that early applied robot-assisted physiotherapy might provide additional treatment benefits in the rehabilitation of post-myocardial infarction (MI) patients. A total of 92 (50 men, 42 women) hospitalized post-MI patients with the age of 60.9 ± 2.32 participated in the research. An early intensive physiotherapy program (7×/week, 2×/day) was applied for each patient with an average time of 45 min per session. Patients were consecutively assigned to Experimental group (EG) and Control group (CG). Then, 20 min of robot-assisted training by Motomed letto 2 or Thera-Trainer tigo was included in all EG physiotherapy sessions. The Functional Independence Measures (FIM) score at the admission and after 14 days of rehabilitation was used for an assessment. When analyzing time * group effect by repeated-measures ANOVA, we reported that EG showed a higher effect in ADL (p = 0.00), and Motor indicators (p = 0.00). There was no statistically significant effect reported in the Social indicator (p = 0.35). Early rehabilitation programs for post-MI patients might be enhanced by robotic tools, such as THERA-Trainer tigo, and Motomed letto 2. The improvement was particularly noticeable in mobility and ADLs.
Trust-Based Research: Influencing Factors of Patients’ Medical Choice Behavior in the Online Medical Community
Authors: Qiu Chutong , Zhang Yuting , Wang Xiaoyu , Gu Dongxiao
Published: May 2022

The medical service is a special credit commodity, and trust plays a very important role in patients’ online medical choice behavior. By collecting information about the doctors on China’s leading online medical platform (Platform A), a regression analysis model was constructed, based on the credibility theory model, which has the following three dimensions: ability trust, benevolence trust, and integrity trust. The results showed that the medical title of the doctors, their department’s reputation, the number of gifts given to them, and the number of patients who registered with them after diagnosis, among other factors, had a significant, positive impact on the behavior of choosing doctors. Among these considerations, the number of patients registered after diagnosis had the greatest impact on the behavior of choosing doctors. This factor is the result of each doctor’s personal brand management, which reflects their comprehensive ability, reputation and integrity. Compared with previous studies, this paper creatively analyzed the important influence of departmental reputation and the number of patients registered after diagnosis on medical choice behavior and puts forward that a doctor can use the number of patients registered after diagnosis to manage their personal brand. Based on the results of this study, we will also put forward suggestions from the perspectives of patients, doctors and the online medical community.
Modulations in Chlorophyll a Fluorescence Based on Intensity and Spectral Variations of Light
Authors: Janeeshma Edappayil , Johnson Riya , Amritha M. S. , Noble Louis , Aswathi K. P. Raj , Telesiński Arkadiusz , Kalaji Hazem M. , Auriga Alicja , Puthur Jos T.
Published: May 2022

Photosynthetic efficiency is significantly affected by both qualitative and quantitative changes during light exposure. The properties of light have a profound effect on electron transport and energy absorption in photochemical reactions. In addition, fluctuations in light intensity and variations in the spectrum can lead to a decrease in photosystem II efficiency. These features necessitate the use of a simple and suitable tool called chlorophyll a fluorescence to study photosynthetic reactions as a function of the aforementioned variables. This research implies that chlorophyll a fluorescence data can be used to determine precise light conditions that help photoautotrophic organisms optimally function.
Characterization of Two Highly Arsenic-Resistant Caulobacteraceae Strains of Brevundimonas nasdae: Discovery of a New Arsenic Resistance Determinant
Authors: Yang Xiaojun , Li Yuanping , Feng Renwei , Chen Jian , Alwathnani Hend A. , Xu Weifeng , Rensing Christopher
Published: May 2022

Arsenic (As), distributed widely in the natural environment, is a toxic substance which can severely impair the normal functions in living cells. Research on the genetic determinants conferring functions in arsenic resistance and metabolism is of great importance for remediating arsenic-contaminated environments. Many organisms, including bacteria, have developed various strategies to tolerate arsenic, by either detoxifying this harmful element or utilizing it for energy generation. More and more new arsenic resistance (ars) determinants have been identified to be conferring resistance to diverse arsenic compounds and encoded in ars operons. There is a hazard in mobilizing arsenic during gold-mining activities due to gold- and arsenic-bearing minerals coexisting. In this study, we isolated 8 gold enrichment strains from the Zijin gold and copper mine (Longyan, Fujian Province, China) wastewater treatment site soil, at an altitude of 192 m. We identified two Brevundimonas nasdae strains, Au-Bre29 and Au-Bre30, among these eight strains, having a high minimum inhibitory concentration (MIC) for As(III). These two strains contained the same ars operons but displayed differences regarding secretion of extra-polymeric substances (EPS) upon arsenite (As(III)) stress. B. nasdae Au-Bre29 contained one extra plasmid but without harboring any additional ars genes compared to B. nasdae Au-Bre30. We optimized the growth conditions for strains Au-Bre29 and Au-Bre30. Au-Bre30 was able to tolerate both a lower pH and slightly higher concentrations of NaCl. We also identified folE, a folate synthesis gene, in the ars operon of these two strains. In most organisms, folate synthesis begins with a FolE (GTP-Cyclohydrolase I)-type enzyme, and the corresponding gene is typically designated folE (in bacteria) or gch1 (in mammals). Heterologous expression of folE, cloned from B. nasdae Au-Bre30, in the arsenic-hypersensitive strain Escherichia coli AW3110, conferred resistance to As(III), arsenate (As(V)), trivalent roxarsone (Rox(III)), pentavalent roxarsone (Rox(V)), trivalent antimonite (Sb(III)), and pentavalent antimonate (Sb(V)), indicating that folate biosynthesis is a target of arsenite toxicity and increased production of folate confers increased resistance to oxyanions. Genes encoding Acr3 and ArsH were shown to confer resistance to As(III), Rox(III), Sb(III), and Sb(V), and ArsH also conferred resistance to As(V). Acr3 did not confer resistance to As(V) and Rox(V), while ArsH did not confer resistance to Rox(V).
Molecular Diagnostic and Prognostication Assays for the Subtyping of Urinary Bladder Cancer Are on the Way to Illuminating Our Vision
Authors: Ecke Thorsten H. , Le Calvez-Kelm Florence , Otto Thomas
Published: May 2022

After the successful publication of three Special Issues devoted to highlighting novel scientific research results in the field of bladder cancer and their clinical implications, we are now directing our efforts towards a fourth edition which will aim at compiling innovative research strategies that will ultimately guide and support clinicians in the decision-making process for targeted bladder cancer therapies [...]
Endothelial Dysfunction Induced by Extracellular Neutrophil Traps Plays Important Role in the Occurrence and Treatment of Extracellular Neutrophil Traps-Related Disease
Authors: Yu Shuyang , Liu Jingyu , Yan Nianlong
Published: May 2022

Many articles have demonstrated that extracellular neutrophil traps (NETs) are often described as part of the antibacterial function. However, since the components of NETs are non-specific, excessive NETs usually cause inflammation and tissue damage. Endothelial dysfunction (ED) caused by NETs is the major focus of tissue damage, which is highly related to many inflammatory diseases. Therefore, this review summarizes the latest advances in the primary and secondary mechanisms between NETs and ED regarding inflammation as a mediator. Moreover, the detailed molecular mechanisms with emphasis on the disadvantages from NETs are elaborated: NETs can use its own enzymes, release particles as damage-associated molecular patterns (DAMPs) and activate the complement system to interact with endothelial cells (ECs), drive ECs damage and eventually aggravate inflammation. In view of the role of NETs-induced ED in different diseases, we also discussed possible molecular mechanisms and the treatments of NETs-related diseases.
A Novel Loss-of-Function SEMA3E Mutation in a Patient with Severe Intellectual Disability and Cognitive Regression
Authors: Paganoni Alyssa J. J. , Amoruso Federica , Porta Pelayo Javier , Calleja-Pérez Beatriz , Vezzoli Valeria , Duminuco Paolo , Caramello Alessia , Oleari Roberto , Fernández-Jaén Alberto , Cariboni Anna
Published: May 2022

Intellectual disability (ID) is a neurological disorder arising from early neurodevelopmental defects. The underlying genetic and molecular mechanisms are complex, but are thought to involve, among others, alterations in genes implicated in axon guidance and/or neural circuit formation as demonstrated by studies on mouse models. Here, by combining exome sequencing with in silico analyses, we identified a patient affected by severe ID and cognitive regression, carrying a novel loss-of-function variant in the semaphorin 3E (SEMA3E) gene, which encodes for a key secreted cue that controls mouse brain development. By performing ad hoc in vitro and ex vivo experiments, we found that the identified variant impairs protein secretion and hampers the binding to both embryonic mouse neuronal cells and tissues. Further, we revealed SEMA3E expression during human brain development. Overall, our findings demonstrate the pathogenic impact of the identified SEMA3E variant and provide evidence that clinical neurological features of the patient might be due to a defective SEMA3E signaling in the brain.
Elevation of Tear MMP-9 Concentration as a Biomarker of Inflammation in Ocular Pathology by Antibody Microarray Immunodetection Assays
Authors: de la Fuente Miguel , Rodríguez-Agirretxe Iñaki , Vecino Elena , Astigarraga Egoitz , Acera Arantxa , Barreda-Gómez Gabriel
Published: May 2022

Matrix metalloproteinases are a family of enzymes fundamental in inflammatory processes. Between them, MMP-9 is up-regulated during inflammation; thus, its quantification in non-invasive fluids is a promising approach for inflammation identification. To this goal, a biomarker quantification test was developed for ocular inflammation detection using anti-MMP-9 antibody microarrays (AbMAs). After validation with eight healthy control tear samples characterized by ELISA, 20 samples were tested from individuals diagnosed with ocular inflammation due to: cataracts, glaucoma, meibomian gland dysfunction, allergy, or dry eye. Concentration values of tear MMP-9 were obtained for each sample, and 12 patients surpassed the pathological threshold (30 ng/mL). A significant elevation of MMP-9 concentration in the tears of glaucoma patients compared with healthy controls was observed. In order to evaluate the diagnostic ability, an ROC curve analysis was performed using our data, determining the optimal threshold for the test at 33.6 ng/mL of tear MMP-9. In addition, a confusion matrix was applied, estimating sensitivity at 60%, specificity at 88%, and accuracy at 68%. In conclusion, we demonstrated that the AbMAs system allows the quantification of MMP-9 in pathologies that involve inflammation of the ocular surface.