bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Steps Registration Protocol for Multimodal MR Images of Hip Skeletal Muscles in a Longitudinal Study
Authors: Fontana Lucia , Mastropietro Alfonso , Scalco Elisa , Peruzzo Denis , Beretta Elena , Strazzer Sandra , Arrigoni Filippo , Rizzo Giovanna
Published: November 2020

Image registration is crucial in multimodal longitudinal skeletal muscle Magnetic Resonance Imaging (MRI) studies to extract reliable parameters that can be used as indicators for physio/pathological characterization of muscle tissue and for assessing the effectiveness of treatments. This paper aims at proposing a reliable registration protocol and evaluating its accuracy in a longitudinal study. The hips of 6 subjects were scanned, in a multimodal protocol, at 2 different time points by a 3 Tesla scanner; the proposed multi-step registration pipeline is based on rigid and elastic transformations implemented in SimpleITK using a multi-resolution technique. The effects of different image pre-processing (muscle masks, isotropic voxels) and different parameters’ values (learning rates and mesh sizes) were quantitatively assessed using standard accuracy indexes. Rigid registration alone does not provide satisfactory accuracy for inter-sessions alignment and a further elastic step is needed. The use of isotropic voxels, combined with the muscle masking, provides the best result in terms of accuracy. Learning rates can be increased to speed up the process without affecting the final results. The protocol described in this paper, complemented by open-source software, can be a useful guide for researchers that approach for the first time the issues related to the muscle MR image registration.
The Biological Function of Extracellular Vesicles during Fertilization, Early Embryo—Maternal Crosstalk and Their Involvement in Reproduction: Review and Overview
Authors: Capra Emanuele , Lange-Consiglio Anna
Published: November 2020

Secretory extracellular vesicles (EVs) are membrane-enclosed microparticles that mediate cell to cell communication in proximity to, or distant from, the cell of origin. Cells release a heterogeneous spectrum of EVs depending on their physiologic and metabolic state. Extracellular vesicles are generally classified as either exosomes or microvesicles depending on their size and biogenesis. Extracellular vesicles mediate temporal and spatial interaction during many events in sexual reproduction and supporting embryo-maternal dialogue. Although many omic technologies provide detailed understanding of the molecular cargo of EVs, the difficulty in obtaining populations of homogeneous EVs makes difficult to interpret the molecular profile of the molecules derived from a miscellaneous EV population. Notwithstanding, molecular characterization of EVs isolated in physiological and pathological conditions may increase our understanding of reproductive and obstetric diseases and assist the search for potential non-invasive biomarkers. Moreover, a more precise vision of the cocktail of biomolecules inside the EVs mediating communication between the embryo and mother could provide new insights to optimize the therapeutic action and safety of EV use.
Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen
Authors: Pasaribu Khatarina Meldawati , Gea Saharman , Ilyas Syafruddin , Tamrin Tamrin , Radecka Izabela
Published: November 2020

Bacterial cellulose (BC), chitosan (Chi), and collagen (Col) are known as biopolymers which have met some properties that are required as wound dressing. This study focused on investigating the fabrication of BC-based wound dressing with chitosan and collagen, since chitosan has red blood cells binding and anti-bacterial properties, while collagen can support cell and tissue growth for skin wounds. The BC-based wound dressing was prepared by impregnating BC fibers in the chitosan and/or collagen solution for 24 h. FTIR was used to confirm the intermolecular interaction of amine and hydroxyl group of chitosan and/or collagen in BC-based wound dressing. Furthermore, the XRD diffractogram of the wound dressing show broader peaks at 14.2°, 16.6°, and 22.4° due to the presence of chitosan and collagen molecules in BC fibers. These results were then supported by SEM images which confirmed that chitosan and collagen were well penetrated into BC fibers. TGA curves revealed that BC/Chi/Col has better thermal properties based on the Tmax compare to BC/Col/Chi. Feasibility of the mats to be applied as wound dressing was also supported by other tests, i.e., water content, porosity, and hemocompatibility, which indicates that the wound dressing is classified as nonhemolytic materials. However, BC/Col/Chi was considered a more potential wound dressing to be applied compared to BC/Chi/Col since it has larger pores and showed better antibacterial properties (larger zones of inhibition) against S. aureus and E. coli via disk diffusion tests.
Working Memory in Children with Learning Disorders: An EEG Power Spectrum Analysis
Authors: Martínez-Briones Benito , Fernández-Harmony Thalía , Garófalo Gómez Nicolás , Biscay-Lirio Rolando , Bosch-Bayard Jorge
Published: November 2020

Learning disorders (LDs) are diagnosed in children whose academic skills of reading, writing or mathematics are impaired and lagging according to their age, schooling and intelligence. Children with LDs experience substantial working memory (WM) deficits, even more pronounced if more than one of the academic skills is affected. We compared the task-related electroencephalogram (EEG) power spectral density of children with LDs (n = 23) with a control group of children with good academic achievement (n = 22), during the performance of a WM task. sLoreta was used to estimate the current distribution at the sources, and 18 brain regions of interest (ROIs) were chosen with an extended version of the eigenvector centrality mapping technique. In this way, we lessened some drawbacks of the traditional EEG at the sensor space by an analysis at the brain-sources level over data-driven selected ROIs. Results: The LD group showed fewer correct responses in the WM task, an overall slower EEG with more delta and theta activity, and less high-frequency gamma activity in posterior areas. We explain these EEG patterns in LD children as indices of an inefficient neural resource management related with a delay in neural maturation.
Recent Advances in Glycerol Catalytic Valorization: A Review
Authors: Checa Manuel , Nogales-Delgado Sergio , Montes Vicente , Encinar José María
Published: November 2020

Once a biorefinery is ready to operate, the main processed materials need to be completely evaluated in terms of many different factors, including disposal regulations, technological limitations of installation, the market, and other societal considerations. In biorefinery, glycerol is the main by-product, representing around 10% of biodiesel production. In the last few decades, the large-scale production of biodiesel and glycerol has promoted research on a wide range of strategies in an attempt to valorize this by-product, with its transformation into added value chemicals being the strategy that exhibits the most promising route. Among them, C3 compounds obtained from routes such as hydrogenation, oxidation, esterification, etc. represent an alternative to petroleum-based routes for chemicals such as acrolein, propanediols, or carboxylic acids of interest for the polymer industry. Another widely studied and developed strategy includes processes such as reforming or pyrolysis for energy, clean fuels, and materials such as activated carbon. This review covers recent advances in catalysts used in the most promising strategies considering both chemicals and energy or fuel obtention. Due to the large variety in biorefinery industries, several potential emergent valorization routes are briefly summarized.
Expanding the Application Range of Microbial Oxidoreductases by an Alcohol Dehydrogenase from Comamonas testosteroni with a Broad Substrate Spectrum and pH Profile
Authors: Bakonyi Daniel , Toelzer Christine , Stricker Michael , Hummel Werner , Niefind Karsten , Gröger Harald
Published: November 2020

Alcohol dehydrogenases catalyse the conversion of a large variety of ketone substrates to the corresponding chiral products. Due to their high regio- and stereospecificity, they are key components in a wide range of industrial applications. A novel alcohol dehydrogenase from Comamonas testosteroni (CtADH) was identified in silico, recombinantly expressed and purified, enzymatically and biochemically investigated as well as structurally characterized. These studies revealed a broad pH profile and an extended substrate spectrum with the highest activity for compounds containing halogens as substituents and a moderate activity for bulky–bulky ketones. Biotransformations with selected ketones—performed with a coupled regeneration system for the co-substrate NADPH—resulted in conversions of more than 99% with all tested substrates and with excellent enantioselectivity for the corresponding S-alcohol products. CtADH/NADPH/substrate complexes modelled on the basis of crystal structures of CtADH and its closest homologue suggested preliminary hints to rationalize the enzyme’s substrate preferences
Minimal Complexity Support Vector Machines for Pattern Classification
Authors: Abe Shigeo
Published: November 2020

Minimal complexity machines (MCMs) minimize the VC (Vapnik-Chervonenkis) dimension to obtain high generalization abilities. However, because the regularization term is not included in the objective function, the solution is not unique. In this paper, to solve this problem, we discuss fusing the MCM and the standard support vector machine (L1 SVM). This is realized by minimizing the maximum margin in the L1 SVM. We call the machine Minimum complexity L1 SVM (ML1 SVM). The associated dual problem has twice the number of dual variables and the ML1 SVM is trained by alternatingly optimizing the dual variables associated with the regularization term and with the VC dimension. We compare the ML1 SVM with other types of SVMs including the L1 SVM using several benchmark datasets and show that the ML1 SVM performs better than or comparable to the L1 SVM.
5-Fluorocytosine/Isocytosine Monohydrate. The First Example of Isomorphic and Isostructural Co-Crystal of Pyrimidine Nucleobases
Authors: Portalone Gustavo
Published: November 2020

To date, despite the crucial role played by cytosine, uracil, and thymine in the DNA/RNA replication process, no examples showing isomorphic and isostructural behavior among binary co-crystals of natural or modified pyrimidine nucleobases have been so far reported in the literature. In view of the relevance of biochemical and pharmaceutical compounds such as pyrimidine nucleobases and their 5-fluoroderivatives, co-crystals of the molecular complex formed by 5-fluorocytosine and isocytosine monohydrate, C4H4FN3O·C4H5N3O·H2O, have been synthesized by a reaction between 5-fluorocytosine and isocytosine. They represent the first example of isomorphic and isostructural binary co-crystals of pyrimidine nucleobases, as X-ray diffraction analysis shows structural similarities in the solid-state organization of molecules with that of the (1:1) 5-fluorocytosine/5-fluoroisocytosine monohydrate molecular complex, which differs solely in the H/F substitution at the C5 position of isocytosine. Molecules of 5-fluorocytosine and isocytosine are present in the crystal as 1H and 3H-ketoamino tautomers, respectively. They form almost coplanar WC base pairs through nucleobase-to-nucleobase DAA/ADD hydrogen bonding interactions, demonstrating that complementary binding enables the crystallization of specific tautomers. Additional peripheral hydrogen bonds involving all available H atom donor and acceptor sites of the water molecule give a three-dimensional polymeric structure. In the absence of H⋯F hydrogen-bonding interactions, the robustness of the supramolecular architectures based on three-point recognition synthons is responsible for the existence of isostructurality between the two molecular complexes.
Microwave-Employed Sol–Gel Synthesis of Scheelite-Type Microcrystalline AgGd(MoO4)2:Yb3+/Ho3+ Upconversion Yellow Phosphors and Their Spectroscopic Properties
Authors: Sung Lim Chang , Aleksandrovsky Aleksandr , Atuchin Victor , Molokeev Maxim , Oreshonkov Aleksandr
Published: November 2020

AgGd(MoO4)2:Ho3+/Yb3+ double molybdates with five concentrations of Ho3+ and Yb3+ were synthesized by the microwave employed sol–gel based process (MES), and the crystal structure variation, concentration effects, and spectroscopic characteristics were investigated. The crystal structures of AgGd1−x−yHoxYby(MoO4)2 (x = 0, 0.05; y = 0, 0.35, 0.4, 0.45, 0.5)at room temperature were determined in space group I41/a by Rietveld analysis. Pure AgGd(MoO4)2 has a scheelite-type structure with mixed occupations of (Ag,Gd) sites and cell parameters a = 5.24782 (11) and c = 11.5107 (3) Å, V = 317.002 (17) Å3, Z = 4. In doped samples, the sites are occupied by a mixture of (Ag,Gd,Ho,Yb) ions, which provides a linear cell volume decrease with the doping level increase. Under the excitation at 980 nm, AGM:0.05Ho,yYb phosphors exhibited a yellowish green emission composed of red and green emission bands according to the strong transitions 5F5 → 5I8 and 5S2/5F4 → 5I8 of Ho3+ ions. The evaluated photoluminescence and Raman spectroscopic results were discussed in detail. The upconversion intensity behavior dependent on the Yb/Ho ratio is explained in terms of the optimal number of Yb3+ ions at the characteristic energy transfer distance around the Ho3+ ion.
Uterine Cervical Neoplasm Diagnosed by Flexible Magnifying Endoscopy with Narrow Band Imaging
Authors: Kobara Hideki , Uchita Kunihisa , Uedo Noriya , Matsuura Noriko , Nishiyama Noriko , Kanenishi Kenji , Masaki Tsutomu
Published: November 2020

When detected early, uterine cervical cancer is one of the most successfully treatable forms of cancer. The diagnostic accuracy of the standard method, the Pap smear test followed by colposcopy, remains unsatisfactory. To improve detection of early-stage cervical cancer, new diagnostic tools for uterine cervical intraepithelial neoplasm (CIN) need to be developed. Magnifying endoscopy with narrow- band imaging (ME-NBI), which allows the visualization of the micro-structure as well as micro-vascularity of the mucosal surface, has excellent diagnostic ability for early gastrointestinal neoplasms. In our previous investigation, ME-NBI was efficacious for diagnosis of CIN. We herein report two notable cases of CIN3 that were diagnosed by ME-NBI that were not detected by colposcopy. These cases illustrate the usefulness of ME-NBI for diagnosis of early-stage uterine cervical neoplasms.
Unpacking Systems of Privilege: The Opportunity of Critical Reflection in Outdoor Adventure Education
Authors: Meerts-Brandsma Lisa , Lackey N. Qwynne , Warner Robert P.
Published: November 2020

Outdoor adventure education has an extensive history of considering how its students should wrestle with privilege. Recent events have brought issues of privilege to the forefront, which raises the question of whether outdoor adventure education can play a role in learning to see and affect systems of privilege. This paper examines several elements of outdoor adventure education that make it an ideal environment for teaching about systems of privilege, and makes the argument that Jack Mezirow’s critical reflection, wherein people question the principles that underlie their ideas, should be a key element of outdoor adventure education curriculum in the 21st century. The authors’ perspectives are grounded in critical theory and the assumption that power dynamics need to be examined in order to be changed. By combining critical reflection with the unique characteristics of outdoor adventure education, outdoor adventure educators may be able to successfully teach participants to recognize and impact systems that operate around them.
Deep Scanning—Beam Selection Based on Deep Reinforcement Learning in Massive MIMO Wireless Communication System
Authors: Kim Minhoe , Lee Woongsup , Cho Dong-Ho
Published: November 2020

In this paper, we investigate a deep learning based resource allocation scheme for massive multiple-input-multiple-output (MIMO) communication systems, where a base station (BS) with a large scale antenna array communicates with a user equipment (UE) using beamforming. In particular, we propose Deep Scanning, in which a near-optimal beamforming vector can be found based on deep Q-learning. Through simulations, we confirm that the optimal beam vector can be found with a high probability. We also show that the complexity required to find the optimum beam vector can be reduced significantly in comparison with conventional beam search schemes.
Data Collection Technology for Ambient Intelligence Systems in Internet of Things
Authors: Vodyaho Alexander , Osipov Vasiliy , Zhukova Nataly , Chernokulsky Vladimir
Published: November 2020

Ambient Intelligence System (AmIS) can be constructed using data collected from Internet of Things (IoT). In this paper, the IoT data collection problem is studied for AmIS with dynamic structure and dynamic behavior of participants (devices), where constraints on resources consumption and performance are essential. A novel technology is proposed, which includes the following steps: (1) definition of the data collection (DC) problem (considering the model of the observed system, DC conditions, etc.); (2) DC policy assignment; (3) construction of DC models; (4) evaluation and presentation of the data processing results. The proposed DC technology supports the development of data collecting subsystems in AmIS. Such subsystems provide data that reflect the changes in structure, state, situation, and behavior of participants in their IoT environment in time. Therefore, we show how this “cognitive” function of the DC process increases the intelligence level of IoT environment.
Analysis of the Development and Parameters of a Public Transport System Which Uses Low-Carbon Energy: The Evidence from Poland
Authors: Patalas-Maliszewska Justyna , Łosyk Hanna
Published: November 2020

Efforts toward a low-emission economy constitute a common challenge for Polish cities. Solutions are being sought to support Polish, medium-sized cities, that is, cities with about 140,000 inhabitants, to implement and develop low-carbon energy in their public transport systems. This paper proposes and explores a sustainable urban development card for a Polish city, namely, Zielona Góra, the use of which will enable the effects of a public transport system using low-carbon energy to be monitored. This research was based on the two main areas of analysis of a system of low-carbon energy and public transport and were formulated as: (1) Sustainable Development Goals (SDGs) and (2) Indicators of the Satisfaction Rate of Public Transport Passengers (SPTP). This paper used literature studies to determine SDGs as well a questionnaire-cum-survey, which was conducted on a sample of 1022 public transport passengers in Zielona Góra, Poland, to determine SPTP. The results were verified by a real case study of a Polish city, which, in 2019, had the largest fleet of electric buses in Poland; a statistical analysis was also conducted using correlation coefficients. It was determined that the proposed approach allows for low carbon energy public transport to be constantly monitored and analyzed. In the long run, this could be a good benchmark as to how cities might improve their level of sustainability.
Plot-Scale Agroforestry Modeling Explores Tree Pruning and Fertilizer Interactions for Maize Production in a Faidherbia Parkland
Authors: Dilla Aynalem M. , Smethurst Philip J. , Huth Neil I. , Barry Karen M.
Published: November 2020

Poor agricultural productivity has led to food shortages for smallholder farmers in Ethiopia. Agroforestry may improve food security by increasing soil fertility, crop production, and livelihoods. Agroforestry simulation models can be useful for predicting the effects of tree management on crop growth when designing modifications to these systems. The Agricultural Production Systems sIMulator (APSIM) agroforestry tree-proxy model was used to simulate the response of maize yield to N fertilizer applications and tree pruning practices in the parkland agroforestry system in the Central Rift Valley, Ethiopia. The model was parameterized and tested using data collected from an experiment conducted under trees and in crop-only plots during the 2015 and 2016 growing seasons. The treatments contained three levels of tree pruning (100% pruned, 50% pruned, and unpruned) as the main plots, and N fertilizers were applied to maize at two rates (9 or 78 kg N ha−1) as sub-plots. Maize yield predictions across two years in response to tree pruning and N applications under tree canopies were satisfactorily simulated (NSE = 0.72, RSR = 0.51, R2 = 0.8). Virtual experiments for different rates of N, pruning levels, sowing dates, and cultivars suggest that maize yield could be improved by applying fertilizers (particularly on crop-only plots) and by at least 50% pruning of trees. Optimal maize yield can be obtained at a higher rate of fertilization under trees than away from them due to better water relations, and there is scope for improving the sowing date and cultivar. Across a 34-year range of recent climate, small increases in yields due to optimum N-fertilizing and pruning were probably limited by nutrient limitations other than N, but the highest yields were consistently in the 2–4 m zone under trees. These virtual experiments helped to form hypotheses regarding fertilizers, pruning, and the effects of trees on soil that warrant further field evaluation.
Impact of the Great East Japan Earthquake on the Employment Status and Mental Health Conditions of Affected Coastal Communities
Authors: Katayanagi Mitsuaki , Seto Moe , Nakaya Naoki , Nakamura Tomohiro , Tsuchiya Naho , Narita Akira , Kogure Mana , Sugawara Yumi , Kodaka Akira , Utsumi Yusuke , Usukura Hitomi , Kunii Yasuto , Hozawa Atsushi , Tsuji Ichiro , Tomita Hiroaki
Published: November 2020

The Great East Japan Earthquake devasted the old community in coastal areas characterized by primary industry. The number of unemployed people increased from 150,000 to 190,000 after the earthquake. All of the adult residents of Shichigahama (18 years old or older), located in the coastal area of the Miyagi prefecture, whose houses were totally or majorly damaged, were recruited for a survey conducted in October 2011. All of the residents who responded with written informed consent were included in this study. Among 904 individuals who had a job before the Great East Japan Earthquake, 19% became unemployed. Concerning gender and age, 9% of young men, 34% of elderly men, 21% of young women, and 49% of elderly women became unemployed. Concerning the type of industry, 38%, 15%, and 16% of people who had belonged to the primary, secondary, and tertiary industries, respectively, before the disaster became unemployed. Those who became unemployed exhibited a significantly higher risk of insomnia compared to those who maintained jobs. The study pointed out the severe impact of the Great East Japan Earthquake on populations who had belonged to the primary industry, especially among elderly women, and its effect on sleep conditions.
Online Relationships and Social Media Interaction in Youth Problem Gambling: A Four-Country Study
Authors: Savolainen Iina , Kaakinen Markus , Sirola Anu , Koivula Aki , Hagfors Heli , Zych Izabela , Paek Hye-Jin , Oksanen Atte
Published: November 2020

The objective of this study was to examine if belonging to online communities and social media identity bubbles predict youth problem gambling. An online survey was administered to 15–25-year-old participants in the United States (N = 1212), South Korea (N = 1192), Spain (N = 1212), and Finland (N = 1200). The survey measured two dimensions of online behavior: perceived sense of belonging to an online community and involvement in social media identity bubbles. Belonging to an online community was examined with a single item and involvement in social media identity bubbles was measured with the six-item Identity Bubble Reinforcement Scale. The South Oaks Gambling Screen was used to assess problem gambling. Statistical analyses utilized linear regression modeling. According to the analyses, strong sense of belonging to an online community was associated with higher problem gambling, but the association was observed mainly among those young individuals who were also involved in social media identity bubbles. For those youths who did not indicate identity bubble involvement, online relationships appeared to function as those offline. Some differences across the four countries were observed but overall, the results indicate that social media identity bubbles could partly explain the harmful influence that some online relations have on youth behavior.
Myths about Intimate Partner Violence and Moral Disengagement: An Analysis of Sociocultural Dimensions Sustaining Violence against Women
Authors: Rollero Chiara , De Piccoli Norma
Published: November 2020

Intimate partner violence (IPV) is a public health issue worldwide and a serious violation of human rights. Recognizing IPV as a form of violence is essential for both victims who need help and offenders who can join treatment programs. Furthermore, only a society able to identify violence can effectively deal with IPV. The present study is aimed at investigating the role of sociocultural dimensions (i.e., ambivalent sexism toward women, ambivalence toward men, and lay theories about gender differences) in sustaining myths about IPV and moral disengagement. The participants were 359 university students (76.5% female). The results show that hostile sexism toward women plays a key role in sustaining both myths and moral disengagement. Moreover, benevolence toward men and biological lay gender theories (i.e., “naïve” theories assuming that sex differences are a product of biology and genetics) significantly affected the endorsement of IPV myths. The implications are discussed.
Mental Healthcare Utilization among Homeless People in the Greater Paris Area
Authors: Dauriac-Le Masson Valérie , Mercuel Alain , Guedj Marie Jeanne , Douay Caroline , Chauvin Pierre , Laporte Anne
Published: November 2020

The healthcare utilization of homeless people is generally considered insufficient, and studies often suffer from methodological bias (institutionalized vs. street samples). To adapt public health policies in France, epidemiological data on this population are scarce. The objective of this study was to analyze the use of psychiatric care by homeless people with mental health problems in the Greater Paris area and to define the factors influencing this use. The data were from the SAMENTA survey performed in 2009 with a representative random street sample of 859 homeless people from the Greater Paris area. The survey studied the use of psychiatric care (lifelong use, current follow-up, discontinuation of follow-up and treatment) and factors potentially associated with this use for people with a diagnosis of a psychotic, mood or anxiety disorder, with the diagnosis established with an original survey device. Because of our complex sampling design, we describe data for only a weighted estimated prevalence, weighted estimation of the number of people in the population (N) and unweighted total subgroup studied in the survey (n). Among 840 homeless people with useable data, 377 (N = 9762) had a psychiatric disorder. The use of whole-life care for these people may seem high, estimated at 68.7%, but few people were followed up for their disorders (18.2%); individuals with a psychotic disorder were more frequently followed up (36.5%) than others were (p < 0.05). Among those followed up (n = 86, N = 1760), 63.0% were taking medication. Access to care for these people seemed preserved, but the maintenance of care seemed problematic; indeed, among people with a lifelong whole use of care (n= 232, N = 6705), 72.3% could be considered to have discontinued care. The factors that improved lifetime health service utilization or follow-up were socio-demographic (age < 42 years, more educated), social (with social security coverage, not living in a hotel), and medical (psychotic disorder, personality disorder, suicide risk, somatic chronic illness, perception of mental suffering). Improving the care of homeless people with psychiatric disorders requires improving access to care for those isolated from the health system (in particular those living in hotels) and to guarantee continuity of care, by adapting the organization of the care system and promoting social rehabilitation.
Working in the Times of COVID-19. Psychological Impact of the Pandemic in Frontline Workers in Spain
Authors: Rodríguez-Rey Rocío , Garrido-Hernansaiz Helena , Bueno-Guerra Nereida
Published: November 2020

This study evaluates the psychological impact (PI) of the COVID-19 pandemic in frontline workers in Spain. Participants were 546 workers (296 healthcare workers, 105 media professionals, 89 grocery workers, and 83 protective service workers). They all completed online questionnaires assessing PI, sadness, concerns related to the COVID-19 pandemic, and demographic and work-related variables. All groups but protective services workers showed higher PI levels than the general population. Healthcare and grocery workers were the most affected, with 73.6% and 65.2% of the participants, respectively, showing a severe PI. Women showed a higher PI level. Healthcare workers in the regions with higher COVID-19 incidences reported greater PI levels. The main concerns were being infected by COVID-19 or infecting others. Levels of concern correlated with higher PI levels. The protection equipment was generally reported as insufficient, which correlated with higher PI levels. Professionals reporting to overwork during the crisis (60% mass-media, 38% of healthcare and grocery and 21.7% of protective service) showed higher PI levels. In the healthcare group, taking care of patients with COVID-19 (77%) or of dying patients with COVID-19 (43.9%) was associated with higher PI levels. The perceived social recognition of their work was inversely related to PI. Most of the sample had not received psychological support. We suggest some organizational measures for frontline institutions, such as the periodical monitoring or inclusion of psychologists specialized in crisis-management to prevent negative symptoms and provide timely support.
Early Gestational Exposure to High-Molecular-Weight Phthalates and Its Association with 48-Month-Old Children’s Motor and Cognitive Scores
Authors: Torres-Olascoaga Libni A. , Watkins Deborah , Schnaas Lourdes , Meeker John D. , Solano-Gonzalez Maritsa , Osorio-Valencia Erika , Peterson Karen E. , Tellez-Rojo Martha María , Tamayo-Ortiz Marcela
Published: November 2020

In utero phthalate exposure has been associated with neurodevelopmental disorders, nevertheless, trimester-specific susceptibility remains understudied. Our aim was to identify susceptible windows to the effects of gestational High-Molecular-Weight Phthalates (HMWP) exposure on 48 months’ neurodevelopment. We measured six HMWP metabolites (MEHP, MEHHP, MEOHP, MECPP, MBzP and MCPP) in urine samples collected during each trimester from women in the Early Life Exposure in Mexico to Environmental Toxicants (ELEMENT) cohort (n = 218). We assessed children’s motor (MS), cognitive (GCI) and memory (MeS) abilities using McCarthy Scales of Children’s Abilities (MSCA). We used linear regression models to examine associations between trimester-specific phthalate metabolites and MSCA scores, adjusted for sex, gestational age, breastfeeding, and maternal IQ. Although phthalate concentrations were similar across trimesters, first and second trimester phthalates were inversely associated with MS and GCI, with first trimester associations with MS being the strongest and statistically significant. Stronger associations were seen with MS and GCI among boys compared to girls, however interaction terms were not statistically significant. Our results suggest that early gestation is a sensitive window of exposure to HMWP for neurodevelopment, particularly in boys. Regulations on phthalate content in food as well as pregnancy consumption guidelines are necessary to protect future generations.
The Carbon Footprint of Valencia Port: A Case Study of the Port Authority of Valencia (Spain)
Authors: Cloquell Ballester Víctor , Lo-Iacono-Ferreira Vanesa G. , Artacho-Ramírez Miguel Ángel , Capuz-Rizo Salvador F.
Published: November 2020

Maritime transport is responsible for 13% of the Greenhouse Gases (GHG) emissions of the transport sector. Port authorities, terminals, shipping companies, and other stakeholders have joined efforts to improve this sector’s environmental performance. In Spain, the Ministry for Ecological Transition and Demographic Challenge has developed a methodology to assess the carbon footprint. This methodology has been adapted to ports and applied to processes under the Port Authority of Valencia’s umbrella achieving scopes 1, 2, and 3. The results highlight that ship traffic, within the port, of containers and cruises (categorized in scope 3) had a major impact on the carbon footprint. Buildings lighting managed by the terminals has a significant effect on scope 2. Diesel consumption shares with gasoline consumption the primary representation in scope 1. The carbon footprint between 2008 and 2016 was maintained, although traffic in the port increased by 24% during this period. The results show a decrease of 17% when emissions are compared using the base year’s emissions factors to avoid external factors. Future projects that include self-consumption or renewable energy policies seem to be the next step in a port that shows good results but still has room for improvement in activities of scope 3.
The Social Distance Scale (v1): A Screening Instrument to Assess Patient Adherence to Prevention Strategies during Pandemics
Authors: Prachthauser Michaela , Cassisi Jeffrey E. , Le Thien-An , Nicasio Andel V.
Published: November 2020

Background: This paper describes the development of a brief self-report screening measure of adherence to social distancing and self-protective behaviors in pandemic situations. Items measured behaviors currently established as primary strategies to prevent and reduce the spread of the COVID-19 infection. Methods: An item pool of 29 questions was generated with the aim of estimating the frequency of specific behaviors and were written to avoid confounding the description of behavioral actions with evaluative judgements. Responses were collected from 401 young adults using an anonymous online survey. Results: An Exploratory Factor Analysis was conducted with the purpose of item reduction and subscale development. A 14-item Social Distance Scale emerged, consisting of four subscales: Isolation from Community (IC), Work from Home (WH), Family Contact (FC), and Protective Behaviors (PB). The initial psychometric evaluation of the scales indicated adequate internal consistency and test-retest reliability. (4) Conclusions: The Social Distance Scale (v1) is a promising new instrument which may be applied at the population or individual level. It may be used in conjunction with COVID-19 testing to measure interactions between social distancing factors and transmission. In addition, a reliable screening measure has utility for health service providers to assess patient risk and to provide educational/counseling.
Soil Mapping Based on Globally Optimal Decision Trees and Digital Imitations of Traditional Approaches
Authors: Zhogolev Arseniy , Savin Igor
Published: November 2020

Most digital soil mapping (DSM) approaches aim at complete statistical model extraction. The value of the explicit rules of soil delineation formulated by soil-mapping experts is often underestimated. These rules can be used for expert testing of the notional consistency of soil maps, soil trend prediction, soil geography investigations, and other applications. We propose an approach that imitates traditional soil mapping by constructing compact globally optimal decision trees (EVTREE) for the covariates of traditionally used soil formation factor maps. We evaluated our approach by regional-scale soil mapping at a test site in the Belgorod region of Russia. The notional consistency and compactness of the decision trees created by EVTREE were found to be suitable for expert-based analysis and improvement. With a large sample set, the accuracy of the predictions was slightly lower for EVTREE (59%) than for CART (67%) and much lower than for Random Forest (87%). With smaller sample sets of 1785 and 1000 points, EVTREE produced comparable or more accurate predictions and much more accurate models of soil geography than CART or Random Forest.
The Genetic Background of Endometriosis: Can ESR2 and CYP19A1 Genes Be a Potential Risk Factor for Its Development?
Authors: Smolarz Beata , Szyłło Krzysztof , Romanowicz Hanna
Published: November 2020

Endometriosis is defined as the presence of endometrial foci, localized beyond their primary site, i.e., the uterine cavity. The etiology of this disease is rather complex. Its development is supported by hormonal, immunological, and environmental factors. During recent years, particular attention has been focused on the genetic mechanisms that may be of particular significance for the increased incidence rates of endometriosis. According to most recent studies, ESR2 and CYP19A1 genes may account for the potential risk factors of infertility associated with endometriosis. The paper presents a thorough review of the latest reports and data concerning the genetic background of the risk for endometriosis development.
A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome
Authors: Chamoun Betty , Caraben Anna , Torres Irina B. , Sellares Joana , Jiménez Raquel , Toapanta Néstor , Cidraque Ignacio , Gabaldon Alejandra , Perelló Manel , Gonzalo Ricardo , O’Valle Francisco , Moreso Francesc , Serón Daniel
Published: November 2020

Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most differentially expressed genes between normal surveillance biopsies (n = 17) and clinical rejection (n = 12) were obtained. A rejection-associated gene (RAG) score was defined as its geometric mean. The following groups were considered: (a) subclinical rejection (REJ-S, n = 6); (b) borderline changes in biopsies for cause (BL-C, n = 13); (c) borderline changes in surveillance biopsies (BL-S, n = 12); (d) IFTA in biopsies for cause (IFTA-C, n = 20); and (e) IFTA in surveillance biopsies (IFTA-S, n = 16). The outcome variable was death-censored graft loss or glomerular filtration rate decline ≥ 30 % at 2 years. A RAG score containing 109 genes derived from normal and clinical rejection (area under the curve, AUC = 1) was employed to classify the study groups. A positive RAG score was observed in 83% REJ-S, 38% BL-C, 17% BL-S, 25% IFTA-C, and 5% IFTA-S. A positive RAG score was an independent predictor of graft outcome from histological diagnosis (hazard ratio: 3.5 and 95% confidence interval: 1.1–10.9; p = 0.031). A positive RAG score predicts graft outcome in surveillance and for cause biopsies with a less severe phenotype than clinical rejection.
Antigen Design for Successful Isolation of Highly Challenging Therapeutic Anti-GPCR Antibodies
Authors: Ju Man-Seok , Jung Sang Taek
Published: November 2020

G-protein-coupled receptors (GPCR) transmit extracellular signals into cells to regulate a variety of cellular functions and are closely related to the homeostasis of the human body and the progression of various types of diseases. Great attention has been paid to GPCRs as excellent drug targets, and there are many commercially available small-molecule chemical drugs against GPCRs. Despite this, the development of therapeutic anti-GPCR antibodies has been delayed and is challenging due to the difficulty in preparing active forms of GPCR antigens, resulting from their low cellular expression and complex structures. Here, we focus on anti-GPCR antibodies that have been approved or are subject to clinical trials and present various technologies to prepare active GPCR antigens that enable the isolation of therapeutic antibodies to proceed toward clinical validation.
Lessons Learned from CNV Analysis of Major Birth Defects
Authors: Hilger Alina Christine , Dworschak Gabriel Clemens , Reutter Heiko Martin
Published: November 2020

The treatment of major birth defects are key concerns for child health. Hitherto, for the majority of birth defects, the underlying cause remains unknown, likely to be heterogeneous. The implicated mortality and/or reduced fecundity in major birth defects suggest a significant fraction of mutational de novo events among the affected individuals. With the advent of systematic array-based molecular karyotyping, larger cohorts of affected individuals have been screened over the past decade. This review discusses the identification of disease-causing copy-number variations (CNVs) among individuals with different congenital malformations. It highlights the differences in findings depending on the respective congenital malformation. It looks at the differences in findings of CNV analysis in non-isolated complex congenital malformations, associated with central nervous system malformations or intellectual disabilities, compared to isolated single organ-system malformations. We propose that the more complex an organ system is, and the more genes involved during embryonic development, the more likely it is that mutational de novo events, comprising CNVs, will confer to the expression of birth defects of this organ system.
Toxicant-Induced Metabolic Alterations in Lipid and Amino Acid Pathways Are Predictive of Acute Liver Toxicity in Rats
Authors: Pannala Venkat R. , Estes Shanea K. , Rahim Mohsin , Trenary Irina , O’Brien Tracy P. , Shiota Chiyo , Printz Richard L. , Reifman Jaques , Shiota Masakazu , Young Jamey D. , Wallqvist Anders
Published: November 2020

Liver disease and disorders associated with aberrant hepatocyte metabolism can be initiated via drug and environmental toxicant exposures. In this study, we tested the hypothesis that gene and metabolic profiling can reveal commonalities in liver response to different toxicants and provide the capability to identify early signatures of acute liver toxicity. We used Sprague Dawley rats and three classical hepatotoxicants: acetaminophen (2 g/kg), bromobenzene (0.4 g/kg), and carbon tetrachloride (0.3 g/kg), to identify early perturbations in liver metabolism after a single acute exposure dose. We measured changes in liver genes and plasma metabolites at two time points (5 and 10 h) and used genome-scale metabolic models to identify commonalities in liver responses across the three toxicants. We found strong correlations for gene and metabolic profiles between the toxicants, indicative of similarities in the liver response to toxicity. We identified several injury-specific pathways in lipid and amino acid metabolism that changed similarly across the three toxicants. Our findings suggest that several plasma metabolites in lipid and amino acid metabolism are strongly associated with the progression of liver toxicity, and as such, could be targeted and clinically assessed for their potential as early predictors of acute liver toxicity.
Effect of Mitochondrial and Cytosolic FXN Isoform Expression on Mitochondrial Dynamics and Metabolism
Authors: Agrò Mauro , Díaz-Nido Javier
Published: November 2020

Friedreich’s ataxia (FRDA) is a neurodegenerative disease caused by recessive mutations in the frataxin gene that lead to a deficiency of the mitochondrial frataxin (FXN) protein. Alternative forms of frataxin have been described, with different cellular localization and tissue distribution, including a cerebellum-specific cytosolic isoform called FXN II. Here, we explored the functional roles of FXN II in comparison to the mitochondrial FXN I isoform, highlighting the existence of potential cross-talk between cellular compartments. To achieve this, we transduced two human cell lines of patient and healthy subjects with lentiviral vectors overexpressing the mitochondrial or the cytosolic FXN isoforms and studied their effect on the mitochondrial network and metabolism. We confirmed the cytosolic localization of FXN isoform II in our in vitro models. Interestingly, both cytosolic and mitochondrial isoforms have an effect on mitochondrial dynamics, affecting different parameters. Accordingly, increases of mitochondrial respiration were detected after transduction with FXN I or FXN II in both cellular models. Together, these results point to the existence of a potential cross-talk mechanism between the cytosol and mitochondria, mediated by FXN isoforms. A more thorough knowledge of the mechanisms of action behind the extra-mitochondrial FXN II isoform could prove useful in unraveling FRDA physiopathology.
Morin Hydrate Sensitizes Hepatoma Cells and Xenograft Tumor towards Cisplatin by Downregulating PARP-1-HMGB1 Mediated Autophagy
Authors: Pal Singh Mahendra , Pal Khaket Tejinder , Bajpai Vivek K. , Alfarraj Saleh , Kim Se-Gie , Chen Lei , Huh Yun Suk , Han Young-Kyu , Kang Sun Chul
Published: November 2020

The cross-talk between apoptosis and autophagy influences anticancer drug sensitivity and cellular death in various cancer cell lines. However, the fundamental mechanisms behind this phenomenon are still unidentified. We demonstrated anti-cancerous role of cisplatin (CP) and morin hydrate (Mh) as an individual and/or in combination (CP-Mh) in hepatoma cells and tumor model. Exposure of CP resulted in the production of intracellular reactive oxygen species (ROS)-mediated cellular vacuolization, expansion of mitochondria membrane and activation of endoplasmic reticulum (ER)-stress. Consequently, Cyt c translocation led to the increase of Bax/Bcl-2 ratio, which simultaneously triggered caspase-mediated cellular apoptosis. In addition, CP-induced PARP-1 activation led to ADP-ribosylation of HMGB1, which consequently developed autophagy as evident by the LC3I/II ratio. Chemically-induced inhibition of autophagy marked by increased cell death signified a protective role of autophagy against CP treatment. CP-Mh abrogates the PARP-1 expression and significantly reduced HMGB1-cytoplasmic translocation with subsequent inhibition of the HMGB1-Beclin1 complex formation. In the absence of PARP-1, a reduced HMGB1 mediated autophagy was observed followed by induced caspase-dependent apoptosis. To confirm the role of PARP-1-HMGB1 signaling in autophagy, we used the PARP-1 inhibitor, 4-amino-1,8-naphthalimide (ANI), HMGB1 inhibitor, ethyl pyruvate (EP), autophagy inhibitors, 3-methyl adenine (3-MA) and bafilomycin (baf) and small interfering RNAs (siRNA) to target Atg5 in combination of CP and Mh. Exposure to these inhibitors enhanced the sensitivity of HepG2 cells to CP. Collectively, our findings indicate that CP-Mh in combination served as a prominent regulator of autophagy and significant inducer of apoptosis that maintains a homeostatic balance towards HepG2 cells and the subcutaneous tumor model.