bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Sustainable Strategies for Mechanical Under-Row Weed Control in the Vineyard
Authors: Gagliardi Lorenzo , Fontanelli Marco , Luglio Sofia Matilde , Frasconi Christian , Peruzzi Andrea , Raffaelli Michele
Published: December 2023

Mechanical under-row weed control in the vineyard emerges as a sustainable choice compared to chemical control, with tillage-based approaches proving especially efficient. A rollhacke, finger weeder, and blade weeder are valid alternatives to commonly used implements that cause excessive soil disruption and display suboptimal efficiency. The trial aimed to compare different under-row weed control strategies in terms of weed control efficacy and operational performance. Among these, in ST1, a tool-holder equipped with both a rollhacke and finger weeder was used at the first and second intervention; in ST2, a rollhacke was used at the first intervention and blade weeder at the second one; in ST3, firstly the tool-holder equipped with a rollhacke and finger weeder was used, then the blade weeder; in ST4, a rollhacke was used first and then the tool-holder equipped with a rollhacke and finger weeder. Weed height, weed cover, and weed biomass were evaluated before the first and after the second intervention. Total field time, fuel consumption, and CO2 emissions of each strategy were assessed. ST1 proved to be the best compromise in terms of weed control effectiveness and operational performance compared to the other strategies. Indeed, ST1 tendentially achieved a lower weed height (20.42 cm) and weed biomass around vine trunks (105.33 g d.m. m−2) compared to the other strategies. In terms of total field time, fuel consumption and CO2 emissions, ST1 recorded intermediate values equal to 3.85 h ha−1, 15.29 kg ha−1, and 48.72 kg ha−1, respectively. Further studies are needed to evaluate these strategies in different vineyard conditions, considering their effect on weed flora composition. Furthermore, exploring automation technology for real-time implement adjustments based on weed infestation levels could further improve the intervention effectiveness and efficiency.
Soybean-Oil-Modified Petrochemical-Source Polyester Polyurethane Improves the Nutrient Release Performance of Coated Urea
Authors: Dong Jingjing , Zhang Zexi , Tian Hongyu , Chen Siyu , Hu Xiaomin , Li Mingyang , Wang Ting , Wan Jinlan , Yu Changxin , Zhu Xiaofan , Chen Qi , Zhang Min , Liu Zhiguang
Published: December 2023

Due to the difficult degradation and high cost of polyester polyols (PPs), their further development in controlled-release fertilizers (CRFs) has been limited. It was of great significance to partially substitute PPs with soybean oil polyols (SOPs) in the preparation of CRFs, which can adjust the proportion of substitution to balance the nutrient release period and membrane degradability. And it is possible to adjust the nitrogen release curve and prepare coated urea with different release days without changing the coating thickness. In this study, a two-factor five-level interaction experiment was designed with different SOP addition ratios (70%, 60%, 50%, 40%, and 30%) and mass ratios of polyols to PAPI (1:1.2; 1:1; 1.2:1; 1.4:1; and 1.6:1). In 25 groups of soybean-oil-modified polyester polyurethane-coated urea (SMPCU) treatments, with the increase in SOP, the thermal decomposition performance of the coated urea was improved, and the residual ash contents of the coating material was reduced. The coating surface was made smoother and denser with a reasonable ratio of polyols to PAPI, preventing the release of non-film-forming substances. When the proportion of soybean oil was 60% and the ratio of polyols to PAPI was 1.2:1, the N release days of the prepared SMPCU reached 137 days. Compared to soybean-oil polyol-coated urea (SOPCU) and polyester polyol-coated urea (PPCU), the nitrogen release days increased by 73.42% (79 days) and 234.15% (41 days), respectively. The ratio of polyols to polyaryl polymethylene isocyanate (PAPI) was explored, as well as the effect of polyol composition on coatings, and prepared SMPCUs with different nitrogen release days. According to the growth needs of crops and soil environmental characteristics, adjusting the proportion of coating materials, prolonging or shortening the nitrogen fertilizer release time, meets the nutritional needs of crops under different planting systems and provides conditions for farmers to plant different crops.
Russian Biodiversity Collections: A Professional Opinion Survey
Authors: Alpeeva Elena V. , Sharova Natalia P. , Sharov Konstantin S. , Vorotelyak Ekaterina A.
Published: December 2023

Biodiversity collections are important vehicles for protecting endangered wildlife in situations of adverse anthropogenic influence. In Russia, there are currently a number of institution- and museum-based biological collections, but there are no nation-wide centres of biodiversity collections. In this paper, we report on the results of our survey of 324 bioconservation, big-data, and ecology specialists from different regions of Russia in regard to the necessity to create several large national biodiversity centres of wildlife protection. The survey revealed specific goals that have to be fulfilled during the development of these centres for the protection and restoration of endangered wildlife species. The top three problems/tasks (topics) are the following: (1) the necessity to create large national centres for different types of specimens; (2) the full sequencing and creation of different “omic” (genomic, proteomic, transcriptomic, etc.) databases; (3) full digitisation of a biodiversity collection/centre. These goals may constitute a guideline for the future of biodiversity collections in Russia that would be targeted at protecting and restoring endangered species. With the due network service level, the translation of the website into English, and permission from the regulator (Ministry of Science and Higher Education of Russian Federation), it can also become an international project.
The Arteries of the Encephalon Base in Caracal (Caracal caracal; Felidae; Carnivora)
Authors: Zdun Maciej , Butkiewicz Aleksander F. , Zawadzki Marcin
Published: December 2023

This study represents the comprehensive anatomical analysis of the arterial circulation at the base of the encephalon in caracal (Caracal caracal), a member of the Felidae family. Caracals are found in various environments in Africa and Asia, and their conservation status is threatened by hunting and habitat loss. This study was conducted on 14 post-mortem specimens obtained from zoos. Three different methods were used to prepare the specimens—corrosive preparation, latex specimen preparation, and computer tomography imaging. This study revealed a configuration of the arterial circulation in the caracal encephalon resembling the shape of the number eight. The presence of the rostral communicating artery in this species is of particular significance, as it is associated with an increased ability to detect dehydration in the forebrain. This adaptation plays a crucial role in responding to challenges related to hydration. Comparative anatomical analysis with other felids highlighted differences in the shape and configuration of the encephalon’s arterial circulation. This study also discussed the obliteration of the extracranial segment of the internal carotid artery in adult caracals, a feature shared with other Felidae members. The results of this study provide valuable information regarding the anatomy of blood vessels in caracals, with potential implications for veterinary practice in zoos and wildlife conservation efforts. This research expands our knowledge of this species’ unique adaptations and physiological processes, contributing to the development of comparative anatomy in the Felidae family.
Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells
Authors: Kalyana Sundaram Shalini devi , Hossain Md. Motaher , Rezki Muhammad , Ariga Kotoko , Tsujimura Seiya
Published: December 2023

Nanomaterials, including carbon nanotubes, graphene oxide, metal–organic frameworks, metal nanoparticles, and porous carbon, play a crucial role as efficient carriers to enhance enzyme activity through substrate channeling while improving enzyme stability and reusability. However, there are significant debates surrounding aspects such as enzyme orientation, enzyme loading, retention of enzyme activity, and immobilization techniques. Consequently, these subjects have become the focus of intensive research in the realm of multi-enzyme cascade reactions. Researchers have undertaken the challenge of creating functional in vitro multi-enzyme systems, drawing inspiration from natural multi-enzyme processes within living organisms. Substantial progress has been achieved in designing multi-step reactions that harness the synthetic capabilities of various enzymes, particularly in applications such as biomarker detection (e.g., biosensors) and the development of biofuel cells. This review provides an overview of recent developments in concurrent and sequential approaches involving two or more enzymes in sequence. It delves into the intricacies of multi-enzyme cascade reactions conducted on nanostructured electrodes, addressing both the challenges encountered and the innovative solutions devised in this field.
Advancements in Wearable EEG Technology for Improved Home-Based Sleep Monitoring and Assessment: A Review
Authors: Mohamed Manal , Mohamed Nourelhuda , Kim Jae Gwan
Published: December 2023

Sleep is a fundamental aspect of daily life, profoundly impacting mental and emotional well-being. Optimal sleep quality is vital for overall health and quality of life, yet many individuals struggle with sleep-related difficulties. In the past, polysomnography (PSG) has served as the gold standard for assessing sleep, but its bulky nature, cost, and the need for expertise has made it cumbersome for widespread use. By recognizing the need for a more accessible and user-friendly approach, wearable home monitoring systems have emerged. EEG technology plays a pivotal role in sleep monitoring, as it captures crucial brain activity data during sleep and serves as a primary indicator of sleep stages and disorders. This review provides an overview of the most recent advancements in wearable sleep monitoring leveraging EEG technology. We summarize the latest EEG devices and systems available in the scientific literature, highlighting their design, form factors, materials, and methods of sleep assessment. By exploring these developments, we aim to offer insights into cutting-edge technologies, shedding light on wearable EEG sensors for advanced at-home sleep monitoring and assessment. This comprehensive review contributes to a broader perspective on enhancing sleep quality and overall health using wearable EEG sensors.
Do Social Support and Loneliness Influence Emerging Adults’ Mental Health during the First Year of the COVID-19 Pandemic?
Authors: Jensen-Campbell Lauri A. , Liegey Dougall Angela , Heller Abigail C. , Iyer-Eimerbrink Priya , Bland Michelle K. , Hull Kristen
Published: December 2023

Youths’ mental health is at a crisis level, with mental health problems doubling in the US since the pandemic began. To compound the mental health crisis, there is a global loneliness epidemic, with emerging adults worldwide experiencing some of the highest rates. One study with two phases examined the influence of social support and loneliness on mental health in US emerging adults during the pandemic, including changes in these relationships over one year. Emerging adults (N = 449) completed online questionnaires via Prolific in May 2020 (Phase 1) and again from January to May 2021 (N = 253; Phase 2). More perceived support was related to reduced loneliness, with family support having the most significant influence. Loneliness mediated the link between perceived support and adverse health outcomes. Higher loneliness predicted more perceived stress and sleep difficulties concurrently and over time. There was a bidirectional relationship between loneliness and depression, such that higher levels of either variable at Time 1 predicted increases in the other over time. Results highlight the detrimental impact of loneliness on emerging adults’ mental health.
Relationships between Math Skills, Motor Skills, Physical Activity, and Obesity in Typically Developing Preschool Children
Authors: Flores Pedro , Coelho Eduarda , Mourão-Carvalhal Isabel , Forte Pedro
Published: December 2023

There is evidence of a relationship between motor and cognitive development. The literature has shown that of all the motor skills, fine motor skills are those that contribute most to mathematical performance in preschool children. As this is a sensitive period in the development of motor skills, low levels of physical activity in this period can compromise their development and contribute to weight gain and obesity. The aim of this study was therefore to analyze the relationship between mathematical and motor skills, physical activity levels, and obesity. The sample consisted of 62 preschool children (32 males) with an average age of 4.63 ± 0.81. The Weschler preschool and primary scale of intelligence—revised arithmetic test was used to assess mathematical skills. The tests to assess fine motor skills were the “Adapted Threading Beads Test” and the “Adapted Visuomotor Integration Test”. The movement assessment battery for children-2, band 1, “Aiming & Catching”, and “Balance” tests were used to assess gross motor skills. Levels of physical activity were assessed using the “Preschool-age physical activity questionnaire” and obesity using the body mass index. The results indicated that only the fine motor skills of visuomotor integration were included in the multiple linear regression model (F < 0.001; r = 0.464; R2 = 0.215; p < 0.001), with the exclusion of gross motor skills, physical activity levels, and obesity levels. Thus, it was concluded that mathematical skills were only directly and significantly influenced by visuomotor integration. However, visuomotor integration was positively and significantly associated with gross motor skills (r = 0.269; p < 0.05) and not with levels of physical activity and obesity. Thus, gross motor skills could contribute to improving visuomotor integration directly and consequently mathematical skills indirectly. The results of this study suggest that the implementation of structured physical activity programs can contribute to mathematical performance.
The Influence of Box-Strengthened Panel Zone on Steel Frame Seismic Performance
Authors: Lu Linfeng , Zhang Jichen , Zhang Gaowei , Peng Hao , Liu Bingyou , Hao Hanlin
Published: December 2023

During the Northridge earthquake, extensive brittle failures on the weld zones of the beam bottom flanges in the rigidity connection of steel special moment frames (SMFs) were detected. One of the primary reasons is the high-tensile strain demand created at the beam bottom flange zones due to positive bending. The weak panel zone of the I-section column exhibits more shear deformation, which promotes and accelerates the brittle fracture of the beam bottom flange weld zones. A box-strengthened panel zone can minimize the shear deformation of the panel zone of the I-section column, which may also reduce the inter-story displacement of steel SMFs and enhance their seismic behavior. In order to investigate this fact, in this research we carried out a model test of a steel frame with a box-strengthened panel zone to examine SMFs’ seismic performance and inter-story displacement, as well as testing the contribution of panel zone shear deformation to inter-story drift. Numerical methods were then used to investigate the influence of the axial compression ratio and beam-to-column linear stiffness ratio on the effect of shear deformation on the box-strengthened panel zone. Design recommendations are given based on the research results.
The Influence of Composition Ratio on the Thermal Performance Parameters of Eutectic Phase Change Materials: Experimental Research and Theoretical Prediction
Authors: Liu Bo , Wang Sheliang , Jia Wurong , Xie Jiangsheng , Lu Zhe , Ying Honghao , Sun Yanwen
Published: December 2023

Eutectic phase change material (EPCM), compared with single phase change material (PCM), is widely studied by many scholars due to its flexibility in practical engineering applications by the characteristic of changing phase change temperatures. However, there is still a lack of theoretical prediction research on the thermal performance parameters of EPCM. The existing theories about EPCM are unable to accurately predict its thermal performance parameters, which increases the difficulty of selecting the composition ratio of EPCM and affects its practical application. Based on a background of the accurate prediction of EPCM thermal parameters, 12 binary EPCMs, and 7 ternary EPCMs are prepared by mixing capric acid, n-octanoic acid and tetradecane with different proportions, respectively. By using the cooling curves and DSC test, the variation patterns in thermal performance parameters of EPCMs are studied. After comparing various thermodynamic models, the Schrader model was selected and combined with experimental results to compare and calculate the experimental and theoretical values of thermal performance parameters. The results show that the binary PCM reaches the minimum eutectic point of 1.42 °C at a mass ratio of 0.33:0.67 for capric acid to n-octanoic acid, while the ternary PCM reaches the minimum eutectic point of 0.34 °C at a mass ratio of 0.231:0.469:0.3 for capric acid, n-octanoic acid, and n-tetradecane. The latent heat of a EPCM is between the lowest and highest values of the latent heat of the constituent components, and its value increases with the enhancement of the overall thermal storage density of the material. The modified Schrader equations can effectively predict the phase change temperatures and latent heats of EPCMs at different composition ratios. The equation has a fitting accuracy of over 0.986 and a bias error of less than 6%, demonstrating excellent accuracy and providing a reliable theoretical basis for the proportion design and thermal parameter prediction of EPCM in actual engineering applications.
Critical Factors for Effective BIM-Enabled Education: An Adaptive Structuration Theory Perspective
Authors: Olowa Theophilus , Witt Emlyn , Lill Irene , Rasheed Abdulkadir , Abdulmumin Abdulazeez , Adebiyi Ranti
Published: December 2023

Educational systems of the 21st century require agility and flexibility for construction graduates to have the skills, knowledge, and abilities required for effective performance in the construction industry, especially with the global digitalization of the industry. With reference to adaptive structuration theory (AST) as a theoretical lens and a survey research design, this research examined the relationship between building information modeling (BIM) as an advanced information technology and educators’ attitudes toward implementing BIM for construction education to prepare local graduates for global relevance and employment. Data collected were analyzed using Statistical Package for the Social Sciences (SPSS Ver 25). The findings of the study showed that group internal system, structure of BIM, and task and organizational environment play important roles in implementing BIM for construction education in the architecture, engineering, construction, and facilities management (AEC/FM) disciplines. It was recommended that policymakers, academics, and curriculum developers pay attention to these factors for rapid development in the realm of BIM education. The development of a robust BIM education framework that considers these factors should also be studied.
Comparative Analysis of Thermal Behavior in Different Seasons in Building Heritage: Case Study of the Royal Hospital of Granada
Authors: Sáez-Pérez María Paz , García Ruiz Luisa María , Durán-Suárez Jorge A. , Castro-Gomes Joao , Martinez-Ramirez Alberto , Villegas-Broncano María Ángeles
Published: December 2023

The present investigation carries out a thermal evaluation of two rooms located in the Royal Hospital of Granada (Rector’s Office). This is a heritage building where have been done studies that allow the as-sessment of possible improvements in future interventions that guarantee improvement in en-ergy and regulatory compliance are decisive. This article presents for the first time, through energy simulation, the behavior of two rooms in two temporal periods, thermally extreme (summer and winter) and with opposite orientations. This has allowed the potential benefits to be considered in real climate conditions. The results demonstrate and quantify that considering the location, orientation, arrangement of openings, and inclusion of transition zones between the exterior and the interior, an improvement in thermal comfort is obtained. The southwesterly orientation is favorable in the winter period and the northeasterly orientation in the summer period. It is also confirmed that the arrangement of thick masonry walls responds adequately in climates with high thermal amplitudes, favoring the mitigation of extreme conditions. It is concluded by stating that the orientation and the construction components are the main responsible factors for the thermal capacity in this type of building. In this context, the use of non-destructive study methods offers valuable scientific support through the results obtained.
Disruption of Mitophagy Flux through the PARL-PINK1 Pathway by CHCHD10 Mutations or CHCHD10 Depletion
Authors: Liu Tian , Wetzel Liam , Zhu Zexi , Kumaraguru Pavan , Gorthi Viraj , Yan Yan , Bukhari Mohammed Zaheen , Ermekbaeva Aizara , Jeon Hanna , Kee Teresa R. , Woo Jung-A Alexa , Kang David E.
Published: December 2023

Coiled-coil-helix-coiled-coil-helix domain-containing 10 (CHCHD10) is a nuclear-encoded mitochondrial protein which is primarily mutated in the spectrum of familial and sporadic amyotrophic lateral sclerosis (ALS)–frontotemporal dementia (FTD). Endogenous CHCHD10 levels decline in the brains of ALS–FTD patients, and the CHCHD10S59L mutation in Drosophila induces dominant toxicity together with PTEN-induced kinase 1 (PINK1), a protein critical for the induction of mitophagy. However, whether and how CHCHD10 variants regulate mitophagy flux in the mammalian brain is unknown. Here, we demonstrate through in vivo and in vitro models, as well as human FTD brain tissue, that ALS/FTD-linked CHCHD10 mutations (R15L and S59L) impair mitophagy flux and mitochondrial Parkin recruitment, whereas wild-type CHCHD10 (CHCHD10WT) normally enhances these measures. Specifically, we show that CHCHD10R15L and CHCHD10S59L mutations reduce PINK1 levels by increasing PARL activity, whereas CHCHD10WT produces the opposite results through its stronger interaction with PARL, suppressing its activity. Importantly, we also demonstrate that FTD brains with TAR DNA-binding protein-43 (TDP-43) pathology demonstrate disruption of the PARL–PINK1 pathway and that experimentally impairing mitophagy promotes TDP-43 aggregation. Thus, we provide herein new insights into the regulation of mitophagy and TDP-43 aggregation in the mammalian brain through the CHCHD10–PARL–PINK1 pathway.
Research on a Multi-Objective Optimization Design for the Durability of High-Performance Fiber-Reinforced Concrete Based on a Hybrid Algorithm
Authors: Wang Xingyu , Cui Fengkun , Cui Long , Jiang Di
Published: December 2023

To achieve durable high-performance fiber-reinforced concrete that meets economic requirements, this paper introduces a hybrid intelligent framework based on the Latin hypercube experimental design, response surface methodology (RSM), and the NSGA-III algorithm for optimizing the mix design of high-performance fiber-reinforced concrete. The developed framework allows for the prediction of concrete performance and obtains a series of Pareto optimal solutions through multi-objective optimization, ultimately identifying the best mix proportion. The decision variables in this optimization are the proportions of various materials in the concrete mix, with concrete’s frost resistance, chloride ion permeability resistance, and cost as the objectives. The feasibility of this framework was subsequently validated. The results indicate the following: (1) The RSM model exhibits a high level of predictive accuracy, with coefficient of determination (R-squared) values of 0.9657 for concrete frost resistance and 0.9803 for chloride ion permeability resistance. The RSM model can be employed to construct the fitness function for the optimization algorithm, enhancing the efficiency of multi-objective optimization. (2) The NSGA-III algorithm effectively balances durability and cost considerations to determine the optimal mix proportion for the concrete. After multi-objective optimization, the chloride ion permeability resistance and frost resistance of the high-performance fiber-reinforced concrete improved by 38.1% and 6.45%, respectively, compared to the experimental averages, while the cost decreased by 2.53%. The multi-objective optimization method proposed in this paper can be applied to mix design for practical engineering projects, improving the efficiency of concrete mix design.
An Alternative to Chlorobenzene as a Hole Transport Materials Solvent for High-Performance Perovskite Solar Cells
Authors: Lee Seung Ho , Lim Seong Bin , Kim Jin Young , Lee Seri , Oh Se Young , Kim Gyu Min
Published: December 2023

Spiro-OMeTAD is a widely used hole-transporting layer (HTL) material, characterized by high hole mobility and good film-forming properties, in perovskite solar cells (PSCs). However, this material has high synthesis costs, low solubility, dependence on hygroscopic dopants, and a low commercial potential. Recently, we investigated alternative materials with good solubility, simple synthetic methods, and good electrical characteristics for use as hole transport materials (HTM) in triple-cation PSCs. Herein, (E,E,E,E)-4,4′,4″,4′″-[Benzene-1,2,4,5-tetrayltetrakis(ethene-2,1-diyl)]tetrakis[N,N-bis(4-methoxyphenyl)aniline], which has a small molecular weight and similar properties to Spiro-OMeTAD, was assessed for use as a HTM via a pre-test of device performance, including its electrical properties, surface morphology, and coating process method, with PSC efficiencies routinely surpassing 20%. A remarkable open-circuit voltage of 1.111, along with a photovoltaic efficiency of 20.18% was obtained in PSCs using this HTM with dichloromethane (DCM) instead of chlorobenzene, indicative of its potential for the fabrication of resistance components with improved surface uniformity. These results provide insights into DCM as an efficient solvent for small molecule-based HTM.
Immunofluorescence-Based Assay for High-Throughput Analysis of Multidrug Resistance Markers in Non-Small Cell Lung Carcinoma Patient-Derived Cells
Authors: Dinić Jelena , Podolski-Renić Ana , Dragoj Miodrag , Jovanović Stojanov Sofija , Stepanović Ana , Lupšić Ema , Pajović Milica , Jovanović Mirna , Petrović Rodić Dušica , Marić Dragana , Ercegovac Maja , Pešić Milica
Published: December 2023

Lung cancer remains the leading cause of cancer death globally, with non-small cell lung cancer (NSCLC) accounting for the majority of cases. Multidrug resistance (MDR), often caused by ATP-binding cassette (ABC) transporters, represents a significant obstacle in the treatment of NSCLC. While genetic profiling has an important role in personalized therapy, functional assays that measure cellular responses to drugs are gaining in importance. We developed an automated microplate-based immunofluorescence assay for the evaluation of MDR markers ABCB1, ABCC1, and ABCG2 in cells obtained from NSCLC patients through high-content imaging and image analysis, as part of a functional diagnostic approach. This assay effectively discriminated cancer from non-cancer cells within mixed cultures, which is vital for accurate assessment of changes in MDR marker expression in different cell populations in response to anticancer drugs. Validation was performed using established drug-sensitive (NCI-H460) and drug-resistant (NCI-H460/R) NSCLC cell lines, demonstrating the assay’s capacity to distinguish and evaluate different MDR profiles. The obtained results revealed wide-ranging effects of various chemotherapeutic agents on MDR marker expression in different patient-derived NSCLC cultures, emphasizing the need for MDR diagnostics in NSCLC. In addition to being a valuable tool for assessing drug effects on MDR markers in different cell populations, the assay can complement genetic profiling to optimize treatment. Further assay adaptations may extend its application to other cancer types, improving treatment efficacy while minimizing the development of resistance.
Comparative Analysis of Estimated and Actual Power Self-Sufficiency Rates in Energy-Sharing Communities with Solar Power Systems
Authors: Kim Dawon , Jang Yonghae , Choi Yosoon
Published: December 2023

Amid the ongoing climate crisis, the international community is enacting policies to promote low-carbon energy-sharing communities. The primary objective of such communities is to enhance community-level energy self-sufficiency. Accurate energy self-sufficiency assessments are paramount in planning energy-efficient architectural designs, urban landscapes, and communal environments. In this study, the energy self-sufficiency rate of an energy-sharing community was estimated at the design stage and compared with the actual energy self-sufficiency rate calculated based on data collected over the following year (April 2022 to March 2023). The outcomes reveal that the estimated energy self-sufficiency rate is 171%, whereas the realized rate is 133%, underscoring the disparity between the projections and outcomes. An analysis of the seasonal variations in these discrepancies elucidated a correlation between the differences in the insolation levels between standard typical meteorological year (TMY) data that are conventionally used for energy generation projections and the actual meteorological conditions. Moreover, a notable incongruity surface exists between the monthly average electricity consumption of a standard four-person household, as stipulated by the Korean Electric Power Corporation (KEPCO) at 273 kWh, and the empirical power consumption at 430 kWh, resulting in a variance of approximately 157 kWh. This study illuminates the complex relationship between variables affecting energy self-sufficiency in energy-sharing communities. It serves as a crucial step towards informed decision making and precision in sustainable urban energy solutions.
Optimal Economic Scheduling Method for Power Systems Based on Whole-System-Cost Electricity Price
Authors: Li Yizheng , Zeng Yuan , Wang Zhidong , Zhao Lang , Wang Yao
Published: December 2023

At present, the traditional scheduling mode of power grids generally dispatches according to the power generation cost within the safe range. Transmission costs are evenly distributed to customers according to their load ratios. There are no methods for the rational distribution of transmission costs according to the utilization degree of generation and load to transmission network resources. This traditional scheduling mode will render transmission cost distribution unfair, and it is difficult to guide reasonable load distribution in time and space. Therefore, an optimal economic scheduling method for power systems based on the whole-system-cost electricity price is proposed in this paper. For the power generation and the transmission sides, the whole-system-cost electricity price model was constructed according to the power flow tracking method. For the load side, a demand-side response model of users’ responses to electricity price changes was established. Finally, the IEEE 57 node standard model was used to simulate optimal economic scheduling. The results show that the proposed method can guide the rational distribution of power flow. The power flow is shifted moderately from far away to near the power generation center, allowing for the load demand to be guided to meet nearby customers’ demands and preventing the line from blocking, the latter of which is conducive to ensuring the safety of the power grid.
Effect of the Slinger Ring on the Forced Convection Heat Transfer in a Window Air Conditioner
Authors: Chang Chin-Hyuk , Jeong Dae-Kwon , Kim Dae-Hyeok , Park Hyun-Cheol , Lee Jong-Ho , Ha Man-Yeong , Yoon Hyun-Sik , Kim Min-Il , Hong Seok-Beom
Published: December 2023

The present study evaluates the effect of the slinger ring on the forced convection heat transfer in window air conditioners. Slinger rings are fitted around condenser fans to spread the condensate onto the condenser to achieve additional cooling. The single-phase forced convection is simulated to compare the thermal performance of the multiphase flow by the slinger ring. Experiments are performed to validate the numerical results. The numerical results well reconstruct the experimental ones, showing the regional dependent distribution and the discharge of the sprayed condensates by the slinger ring. The slinger ring causes a considerable heat transfer on the condenser coils by spraying the condensates, compared with the single-phase flow. However, the inner region of the slinger ring and the fan is almost the dead zone for the condensate spray, since the strong axial flow protects the entrainment of the splashed condensate, which is explained by the isotherms and velocity vectors. The regions of the occurrence of the additional heat transfer are almost overlapped to those exposed to the condensates sprayed by the slinger ring. The slinger ring contributes to a substantial increase of approximately 17% in the heat transfer on the condenser coils, compared with the single-phase flow.
Feasibility of Bonding High-Moisture-Content Wood Using Nothofagus chilean Species
Authors: Rosales Víctor , Rodríguez-Grau Gonzalo , Galarce Carlos , Montero Claudio , Alvarado Claudia , Muñoz Lisa , Pommier Régis
Published: December 2023

Appraising and protecting forests requires a management plan and the creation of innovative products for the market. The development of the green gluing technique could add value to native timber. However, there is a lack of knowledge concerning the response and the productive process of Nothofagus species using this technique. This work investigated the viability of implementing the green gluing method using three types of Nothofagus. Wood pieces were made using a one-component polyurethane adhesive. Delamination, shear tests, morphological characterization, and bond line thickness analysis tested their capacity. The results showed a variable response depending on the Nothofagus type, where the surface treatment could improve the green gluing performance. The findings highlight the relevance of increasing knowledge about the essayed species and their preparation to maintain their natural moisture condition.
Characterization of the IAA-Producing and -Degrading Pseudomonas Strains Regulating Growth of the Common Duckweed (Lemna minor L.)
Authors: Popržen Tatjana , Nikolić Ivan , Krstić-Milošević Dijana , Uzelac Branka , Trifunović-Momčilov Milana , Marković Marija , Radulović Olga
Published: December 2023

The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four Pseudomonas strains isolated from the rhizosphere of the common duckweed (Lemna minor) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. P. putida A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, P. gessardii C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).
Mitochondrial p38 Mitogen-Activated Protein Kinase: Insights into Its Regulation of and Role in LONP1-Deficient Nematodes
Authors: Taouktsi Eirini , Kyriakou Eleni , Voulgaraki Evangelia , Verganelakis Dimitris , Krokou Stefania , Rigas Stamatis , Voutsinas Gerassimos E. , Syntichaki Popi
Published: December 2023

p38 Mitogen-Activated Protein Kinase (MAPK) cascades are central regulators of numerous physiological cellular processes, including stress response signaling. In C. elegans, mitochondrial dysfunction activates a PMK-3/p38 MAPK signaling pathway (MAPKmt), but its functional role still remains elusive. Here, we demonstrate the induction of MAPKmt in worms deficient in the lonp-1 gene, which encodes the worm ortholog of mammalian mitochondrial LonP1. This induction is subjected to negative regulation by the ATFS-1 transcription factor through the CREB-binding protein (CBP) ortholog CBP-3, indicating an interplay between both activated MAPKmt and mitochondrial Unfolded Protein Response (UPRmt) surveillance pathways. Our results also reveal a genetic interaction in lonp-1 mutants between PMK-3 kinase and the ZIP-2 transcription factor. ZIP-2 has an established role in innate immunity but can also modulate the lifespan by maintaining mitochondrial homeostasis during ageing. We show that in lonp-1 animals, ZIP-2 is activated in a PMK-3-dependent manner but does not confer increased survival to pathogenic bacteria. However, deletion of zip-2 or pmk-3 shortens the lifespan of lonp-1 mutants, suggesting a possible crosstalk under conditions of mitochondrial perturbation that influences the ageing process. Furthermore, loss of pmk-3 specifically diminished the extreme heat tolerance of lonp-1 worms, highlighting the crucial role of PMK-3 in the heat shock response upon mitochondrial LONP-1 inactivation.
Risk Polymorphisms of FNDC5, BDNF, and NTRK2 and Poor Education Interact and Aggravate Age-Related Cognitive Decline
Authors: Tomás Alessandra Mendonça , Bento-Torres Natáli Valim Oliver , Jardim Naina Yuki Vieira , Moraes Patrícia Martins , da Costa Victor Oliveira , Modesto Antônio Conde , Khayat André Salim , Bento-Torres João , Picanço-Diniz Cristovam Wanderley
Published: December 2023

Cognitive abilities tend to decline with aging, with variation between individuals, and many studies seek to identify genetic biomarkers that more accurately anticipate risks related to pathological aging. We investigated the influence of BDNF, NTRK2, and FNDC5 single nucleotide polymorphisms (SNPs) on the cognitive performance of young and older adults with contrasting educational backgrounds. We addressed three questions: (1) Is education associated with reduced age-related cognitive decline? (2) Does the presence of SNPs explain the variation in cognitive performance observed late in life? (3) Is education differentially associated with cognition based on the presence of BDNF, NTRK2, or FNDC5 polymorphisms? We measured the cognitive functions of young and older participants, with lower and higher education, using specific and sensitive tests of the Cambridge Automated Neuropsychological Test Assessment Battery. A three-way ANOVA revealed that SNPs were associated with differential performances in executive functions, episodic memory, sustained attention, mental and motor response speed, and visual recognition memory and that higher educational levels improved the affected cognitive functions. The results revealed that distinct SNPs affect cognition late in life differentially, suggesting their utility as potential biomarkers and emphasizing the importance of cognitive stimulation that advanced education early in life provides.
Phosphate (Pi) Transporter PIT1 Induces Pi Starvation in Salmonella-Containing Vacuole in HeLa Cells
Authors: Yang Wen , Feng Yingxing , Yan Jun , Kang Chenbo , Yao Ting , Sun Hongmin , Cheng Zhihui
Published: December 2023

Salmonella enterica serovar Typhimurium (S. Typhimurium), an important foodborne pathogen, causes diarrheal illness and gastrointestinal diseases. S. Typhimurium survives and replicates in phagocytic and non-phagocytic cells for acute or chronic infections. In these cells, S. Typhimurium resides within Salmonella-containing vacuoles (SCVs), in which the phosphate (Pi) concentration is low. S. Typhimurium senses low Pi and expresses virulence factors to modify host cells. However, the mechanism by which host cells reduce the Pi concentration in SCVs is not clear. In this study, we show that through the TLR4-MyD88-NF-κB signaling pathway, S. Typhimurium upregulates PIT1, which in turn transports Pi from SCVs into the cytosol and results in Pi starvation in SCVs. Immunofluorescence and western blotting analysis reveal that after the internalization of S. Typhimurium, PIT1 is located on SCV membranes. Silencing or overexpressing PIT1 inhibits or promotes Pi starvation, Salmonella pathogenicity island-2 (SPI-2) gene expression, and replication in SCVs. The S. Typhimurium ΔmsbB mutant or silenced TLR4-MyD88-NF-κB pathway suppresses the expression of the SPI-2 genes and promotes the fusion of SCVs with lysosomes. Our results illustrate that S. Typhimurium exploits the host innate immune responses as signals to promote intracellular replication, and they provide new insights for the development of broad-spectrum therapeutics to combat bacterial infections.
Sialyltransferase Mutations Alter the Expression of Calcium-Binding Interneurons in Mice Neocortex, Hippocampus and Striatum
Authors: Blažetić Senka , Krajina Vinko , Labak Irena , Viljetić Barbara , Pavić Valentina , Ivić Vedrana , Balog Marta , Schnaar Ronald L. , Heffer Marija
Published: December 2023

Gangliosides are major glycans on vertebrate nerve cells, and their metabolic disruption results in congenital disorders with marked cognitive and motor deficits. The sialyltransferase gene St3gal2 is responsible for terminal sialylation of two prominent brain gangliosides in mammals, GD1a and GT1b. In this study, we analyzed the expression of calcium-binding interneurons in primary sensory (somatic, visual, and auditory) and motor areas of the neocortex, hippocampus, and striatum of St3gal2-null mice as well as St3gal3-null and St3gal2/3-double null. Immunohistochemistry with highly specific primary antibodies for GABA, parvalbumin, calretinin, and calbindin were used for interneuron detection. St3gal2-null mice had decreased expression of all three analyzed types of calcium-binding interneurons in all analyzed regions of the neocortex. These results implicate gangliosides GD1a and GT1b in the process of interneuron migration and maturation.
Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.)
Authors: Liu Congge , Cheng Haijing , Wang Shuwei , Yu Dashi , Wei Yunmin
Published: December 2023

Aluminum (Al) toxicity is the most common factor limiting the growth of alfalfa in acidic soil conditions. Melatonin (MT), a significant pleiotropic molecule present in both plants and animals, has shown promise in mitigating Al toxicity in various plant species. This study aims to elucidate the underlying mechanism by which melatonin alleviates Al toxicity in alfalfa through a combined physiological and transcriptomic analysis. The results reveal that the addition of 5 μM melatonin significantly increased alfalfa root length by 48% and fresh weight by 45.4% compared to aluminum treatment alone. Moreover, the 5 μM melatonin application partially restored the enlarged and irregular cell shape induced by aluminum treatment, resulting in a relatively compact arrangement of alfalfa root cells. Moreover, MT application reduces Al accumulation in alfalfa roots and shoots by 28.6% and 27.6%, respectively. Additionally, MT plays a crucial role in scavenging Al-induced excess H2O2 by enhancing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), consequently reducing malondialdehyde (MDA) levels. More interestingly, the RNA-seq results reveal that MT application significantly upregulates the expression of xyloglucan endotransglucosylase/hydrolase (XTH) and carbon metabolism-related genes, including those involved in the glycolysis process, as well as sucrose and starch metabolism, suggesting that MT application may mitigate Al toxicity by facilitating the binding of Al to the cell walls, thereby reducing intracellular Al accumulation, and improving respiration and the content of sucrose and trehalose. Taken together, our study demonstrates that MT alleviates Al toxicity in alfalfa by reducing Al accumulation and restoring redox homeostasis. These RNA-seq results suggest that the alleviation of Al toxicity by MT may occur through its influence on cell wall composition and carbon metabolism. This research advances our understanding of the mechanisms underlying MT’s effectiveness in mitigating Al toxicity, providing a clear direction for our future investigations into the underlying mechanisms by which MT alleviates Al toxicity in alfalfa.
Reprogramming in Candida albicans Gene Expression Network under Butanol Stress Abrogates Hyphal Development
Authors: Anand Rajesh , Kashif Mohammad , Pandit Awadhesh , Babu Ram , Singh Agam P.
Published: December 2023

Candida albicans is the causative agent of invasive fungal infections. Its hyphae-forming ability is regarded as one of the important virulence factors. To unravel the impact of butanol on Candida albicans, it was placed in O+ve complete human serum with butanol (1% v/v). The Candida transcriptome under butanol stress was then identified by mRNA sequencing. Studies including electron microscopy demonstrated the inhibition of hyphae formation in Candida under the influence of butanol, without any significant alteration in growth rate. The numbers of genes upregulated in the butanol in comparison to the serum alone were 1061 (20 min), 804 (45 min), and 537 (120 min). Candida cells exhibited the downregulation of six hypha-specific transcription factors and the induction of four repressor/regulator genes. Many of the hypha-specific genes exhibited repression in the medium with butanol. The genes related to adhesion also exhibited repression, whereas, among the heat-shock genes, three showed inductions in the presence of butanol. The fungal-specific genes exhibited induction as well as repression in the butanol-treated Candida cells. Furthermore, ten upregulated genes formed the core stress gene set in the presence of butanol. In the gene ontology analysis, enrichment of the processes related to non-coding RNA, ribosome biosynthesis, and metabolism was observed in the induced gene set. On the other side, a few GO biological process terms, including biofilm formation and filamentous growth, were enriched in the repressed gene set. Taken together, under butanol stress, Candida albicans is unable to extend hyphae and shows growth by budding. Many of the genes with perturbed expression may have fitness or virulence attributes and may provide prospective sites of antifungal targets against C. albicans.