bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Implications of Genomic Loss of Heterozygosity in Endometrial Carcinoma
Authors: Bustamante Bethany , Sinha Risha , Rice Briana , Nizam Aaron , Shan Weiwei , Goldberg Gary L. , John Veena , Lin Doug I. , Danziger Natalie , Pavlick Dean C. , Elvin Julia A. , Frimer Marina
Published: June 2021

PURPOSE

Homologous recombination deficiency, identified by homologous recombination deficiency gene alterations or high percentage of genome-wide loss of heterozygosity (gLOH), is associated with improved prognosis, platinum sensitivity (PS), and poly (ADP-ribose) polymerase inhibitor response in high-grade ovarian cancer. Since the copy number–high (CN-H) endometrial cancer molecular subtype (EC-MS) shares molecular features with high-grade ovarian cancer, our aim was to assign EC-MS on the basis of comprehensive genomic profiling (CGP) results and evaluate the gLOH status with clinical behavior of EC.

METHODS

Eighty-two epithelial EC tumor tissues were sequenced by hybrid capture–based CGP, and results were used to assign EC-MS (ultramutated, microsatellite instability–high, CN-low; CN-high). Retrospective chart review established clinical characteristics, including PS. Relationships of PS, EC-MS, gene alterations, and gLOH were assessed statistically.

RESULTS

PS and EC-MS of CN-H showed statistically significant difference in overall survival (OS). Most notably, when the CN-H EC-MS was subcategorized by gLOH status, there was a significant difference in OS with gLOH-H being associated with longer survival. Cox semi-proportional hazard modeling showed that gLOH, stage, and race were significant in modeling OS.

CONCLUSION

The method of assigning EC-MS by CGP demonstrates similar clinical features to previous reports of EC-MS assigned by other methods. CGP can also assess gLOH status with gLOH-H most commonly seen in CN-H tumors. CN-H, gLOH-H patients showed significantly improved OS (hazard ratio, 0.100 [0.02-0.51 95% CI]). Thus, gLOH status may be a meaningful prognostic biomarker within the CN-H tumors and possibly across EC-MS.
Pro-Con Debate: Fibrinogen Concentrate or Cryoprecipitate for Treatment of Acquired Hypofibrinogenemia in Cardiac Surgical Patients
Authors: Hensley Nadia B. , Mazzeffi Michael A.
Published: June 2021

Cryoprecipitate has been the gold standard for treating acquired hypofibrinogenemia in cardiac surgery for nearly 50 years. More recently, fibrinogen concentrate has been used off-label in the United States and is the standard in European countries and Canada to treat the acquired hypofibrinogenemia during cardiac surgery. Fibrinogen concentrate has multiple potential advantages including rapid reconstitution, greater dose predictability, viral inactivation during processing, and reduced transfusion-related adverse events. However, because fibrinogen concentrate lacks the other components contained in the cryoprecipitate, it may not be the “ideal” product for replacing fibrinogen in all cardiac surgical patients, particularly those with longer cardiopulmonary bypass duration. In this Pro-Con commentary article, we discuss the advantages and disadvantages of using fibrinogen concentrate and cryoprecipitate to treat acquired hypofibrinogenemia in cardiac surgical patients.
Qualities of Effective Vital Anaesthesia Simulation Training Facilitators Delivering Simulation-Based Education in Resource-Limited Settings
Authors: Mossenson Adam I. , Bailey Jonathan G. , Whynot Sara , Livingston Patricia
Published: June 2021

BACKGROUND:

Lack of access to safe and affordable anesthesia and surgical care is a major contributor to avoidable death and disability across the globe. Effective education initiatives are a viable mechanism to address critical skill and process gaps in perioperative teams. Vital Anaesthesia Simulation Training (VAST) aims to overcome barriers limiting widespread application of simulation-based education (SBE) in resource-limited environments, providing immersive, low-cost, multidisciplinary SBE and simulation facilitator training. There is a dearth of knowledge regarding the factors supporting effective simulation facilitation in resource-limited environments. Frameworks evaluating simulation facilitation in high-income countries (HICs) are unlikely to fully assess the range of skills required by simulation facilitators working in resource-limited environments. This study explores the qualities of effective VAST facilitators; knowledge gained will inform the design of a framework for assessing simulation facilitators working in resource-limited contexts and promote more effective simulation faculty development.

METHODS:

This qualitative study used in-depth interviews to explore VAST facilitators’ perspectives on attributes and practices of effective simulation in resource-limited settings. Twenty VAST facilitators were purposively sampled and consented to be interviewed. They represented 6 low- and middle-income countries (LMICs) and 3 HICs. Interviews were conducted using a semistructured interview guide. Data analysis involved open coding to inductively identify themes using labels taken from the words of study participants and those from the relevant literature.

RESULTS:

Emergent themes centered on 4 categories: Persona, Principles, Performance and Progression. Effective VAST facilitators embody a set of traits, style, and personal attributes (Persona) and adhere to certain Principles to optimize the simulation environment, maximize learning, and enable effective VAST Course delivery. Performance describes specific practices that well-trained facilitators demonstrate while delivering VAST courses. Finally, to advance toward competency, facilitators must seek opportunities for skill Progression.

Interwoven across categories was the finding that effective VAST facilitators must be cognizant of how context, culture, and language may impact delivery of SBE. The complexity of VAST Course delivery requires that facilitators have a sensitive approach and be flexible, adaptable, and open-minded. To progress toward competency, facilitators must be open to self-reflection, be mentored, and have opportunities for practice.

CONCLUSIONS:

The results from this study will help to develop a simulation facilitator evaluation tool that incorporates cultural sensitivity, flexibility, and a participant-focused educational model, with broad relevance across varied resource-limited environments.
TEAD family transcription factors in development and disease
Authors: Currey Laura , Thor Stefan , Piper Michael
Published: June 2021

The balance between stem cell potency and lineage specification entails the integration of both extrinsic and intrinsic cues, which ultimately influence gene expression through the activity of transcription factors. One example of this is provided by the Hippo signalling pathway, which plays a central role in regulating organ size during development. Hippo pathway activity is mediated by the transcriptional co-factors Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ), which interact with TEA domain (TEAD) proteins to regulate gene expression. Although the roles of YAP and TAZ have been intensively studied, the roles played by TEAD proteins are less well understood. Recent studies have begun to address this, revealing that TEADs regulate the balance between progenitor self-renewal and differentiation throughout various stages of development. Furthermore, it is becoming apparent that TEAD proteins interact with other co-factors that influence stem cell biology. This Primer provides an overview of the role of TEAD proteins during development, focusing on their role in Hippo signalling as well as within other developmental, homeostatic and disease contexts.
Laminin alpha 5 regulates mammary gland remodeling through luminal cell differentiation and Wnt4-mediated epithelial crosstalk
Authors: Englund Johanna I. , Ritchie Alexandra , Blaas Leander , Cojoc Hanne , Pentinmikko Nalle , Döhla Julia , Iqbal Sharif , Patarroyo Manuel , Katajisto Pekka
Published: June 2021

Epithelial attachment to the basement membrane (BM) is essential for mammary gland development, yet the exact roles of specific BM components remain unclear. Here, we show that Laminin α5 (Lama5) expression specifically in the luminal epithelial cells is necessary for normal mammary gland growth during puberty, and for alveologenesis during pregnancy. Lama5 loss in the keratin 8-expressing cells results in reduced frequency and differentiation of hormone receptor expressing (HR+) luminal cells. Consequently, Wnt4-mediated crosstalk between HR+ luminal cells and basal epithelial cells is compromised during gland remodeling, and results in defective epithelial growth. The effects of Lama5 deletion on gland growth and branching can be rescued by Wnt4 supplementation in the in vitro model of branching morphogenesis. Our results reveal a surprising role for BM-protein expression in the luminal mammary epithelial cells, and highlight the function of Lama5 in mammary gland remodeling and luminal differentiation.
The molecular complex of ciliary and golgin protein is crucial for skull development
Authors: Yamaguchi Hiroyuki , Meyer Matthew D. , He Li , Senavirathna Lakmini , Pan Sheng , Komatsu Yoshihiro
Published: June 2021

Intramembranous ossification, which consists of direct conversion of mesenchymal cells to osteoblasts, is a characteristic process in skull development. One crucial role of these osteoblasts is to secrete collagen-containing bone matrix. However, it remains unclear how the dynamics of collagen trafficking is regulated during skull development. Here, we reveal the regulatory mechanisms of ciliary and golgin proteins required for intramembranous ossification. During normal skull formation, osteoblasts residing on the osteogenic front actively secreted collagen. Mass spectrometry and proteomic analysis determined endogenous binding between ciliary protein IFT20 and golgin protein GMAP210 in these osteoblasts. As seen in Ift20 mutant mice, disruption of neural crest-specific GMAP210 in mice caused osteopenia-like phenotypes due to dysfunctional collagen trafficking. Mice lacking both IFT20 and GMAP210 displayed more severe skull defects compared with either IFT20 or GMAP210 mutants. These results demonstrate that the molecular complex of IFT20 and GMAP210 is essential for the intramembranous ossification during skull development.
A draft reference assembly of the Psilocybe cubensis genome
Authors: McKernan Kevin , Kane Liam T. , Crawford Seth , Chin Chen-Shan , Trippe Aaron , McLaughlin Stephen
Published: June 2021

We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.
Pre-clinical undergraduate students’ perspectives on the adoption of virtual and augmented reality to their dental learning experience: A one-group pre- and post-test design protocol
Authors: Afrashtehfar Kelvin I. , Yang Jing-Wen , Al-Sammarraie A. , Chen Hui , Saeed Musab H.
Published: June 2021

Background: We live in a time where traditional education has rapidly incorporated online modalities due to the recent SARS-CoV-2 (COVID-19) safety measures such as social distancing. Regardless of these challenges, health education constantly strives to implement the best technologies available for an effective student deep learning outcome. Virtual (VR) and augmented reality (AR) in the dental pre-clinical stage may help stimulate students to better understand the foundation material prescribed in the curriculum. Most visual material available for students is still mainly based on 2D graphics. Thus, this study will attempt to evaluate the students' perceptions about implementing VR/AR technologies in the learning setting.

Methods: A single-group pretest-posttest design will be implemented where students will be exposed to VR/AR and fill out two questionnaires, one before and one after the exposure.

Conclusions: This project is intended to start once the institutional ethical approval is obtained. It is expected that the analysis from the current project will provide recommendations to improve the students' academic curriculum pre-clinical experience. The recommendations will be provided in the form of at least three scientific publications, with one publication for each subject area intended to be evaluated (i.e., head and neck anatomy, dental anatomy, and removable prosthodontics).
do poder disciplinar ao biopoder à necropolítica: a criança negra em busca de uma infância descolonizada
Authors: Gomes Nilma Lino , Teodoro Cristina
Published: June 2021

The article discusses how the emergence of the term "minor" in Brazilian society, from the mid-nineteenth century, forged mainly by the practice of legal and medical discourse, outlined a path of institutionalization for poor children; first, in the post-abolition period  of enslavement until the thirties of the last century and, later, until the period of democratization of Brazil, considering the promulgation of the Federal Constitution of 1988. The analysis for the proposed periods was based on the concept of disciplinary power and biopower, both coined by Foucault. Furthermore, the emergence of the black child was discussed, based on its status as a citizen child, contained in the Federal Constitution of 1988, and in the Statute of the Child and Adolescent created in the 1990s. From this period up to the present day, the text deals with the new devices created by the Brazilian State, which have ensured unequal conditions for black children and mainly promoted the increase of homicides among them.The analysis of the last period presented was based on the concept of “necropolitics,” developed by Achille Mbembe. Finally, we defend the principle that another childhood for black child will only be possible through a becoming-other, a new opening of the world and, above all, a decolonization of childhood for children belonging to the black ethnic-racial group.
Peculiarities of teaching professionally оriented oral communication of nonlinguistic university senior students
Authors:   State Institution Luhansk Taras Shevchenko National University, Demchenko N. A.
Published: June 2021

The article deals with the problem of teaching students (for whom foreign language is not a specialty) an oral foreign language professional communication. A number of scholars’ woks, including methodologists and linguists devoted to this topic were analyzed. Numerous studies are dedicated to the problem of teaching oral speech in a nonlinguistic university but most of them are often concern only with the initial stage of education. Less attention is given to the problem of teaching senior students professionally oriented oral communication. Professionally oriented teaching refers to training based on the needs of students in teaching a foreign language dictated by the characteristics of the future profession or specialty. It involves the combination of mastering a professionally oriented foreign language with the development of the personal qualities of students as well as with the acquisition of special skills based on professional and linguistic knowledge. It is necessary to highlight the communicative intentions of business communication among other communicative intentions. A typical example of the natural conditions of communication in the specialty is the defense of master's projects. Oral speech during the defense of master's projects is a conversation, a special type of dialogue between the master and the examiner, which is based on the examiner's questions and the master's answers. This type of communication is typical for other situations of communication between specialists. As a result of the study, we came to the conclusion that the basis of the linguistic material in the specialty can be some complexes of information on certain topics composed of texts of different types of technical literature and presented not only in the form of printed sources but also audiovisual materials. The topics of oral speech, the choice of speech means to a large extent will be determined by the range of so-called selected problem questions-tasks. Training specialists in a non-linguistic university is carried out within a limited time as well as on a rather limited material therefore mastering spontaneous speech is quite problematic.
The syntax of a simple sentence in the poetry of Yevhen Malanyuk
Authors:   Dnipropetrovsk State University of Internal Affairs, Podvorchan A. Z.
Published: June 2021

The article considers the peculiarities of the syntax in the simple sentence on the basis of small forms poetry which was written by Yevhen Malanyuk. It is analyzed and established that the stylistic resources of a simple sentence include special cases of using homogeneity, varieties of simple sentence models, various complicating components. Incomplete sentences are the most expressive syntactic constructions in Yevhen Malanyuk's poetic texts. It was found out, that the poet often uses the inversion, so in the sentence he changes the parts of the sentence in order to elicit different emotions (e.g. positive or negative feelings) of the reader, to focus his attention on a particular parts of the sentence. Also the author uses productive sentence models, but it is established that simple sentences with complex components are dominated in this way. The study clarifies the features of syntactic and stylistic organization of the language of poetic works, such as the specifics of subject and predicate coordination, cases of pictorial use of homogeneous parts of the sentence, the expressive possibilities of a simple sentence in poetic texts. The result of this work highlighted the features of syntactic and stylistic organization of the poetic text of the author. Thus, the study make it possible to recognize the contribution of the author in the development of syntactic system resources of Ukrainian artistic speech. Also, it helps to determine the patterns and trends in the development of literary language. The research of the syntactic linguistic aspect of Yevhen Malanyuk highlights the individual uniqueness of the poet. The perspective of the study is to research the syntax of a complex sentence in the works of Ye. Malaniuk. Besides to understand the features of the language of the poet's language as a unique phenomenon in the history of the Ukrainian literary language.
English proverbs as explicators of coordinate phrases with the adversative conjunction but and the cumulative conjunction and
Authors:   State Institution Luhansk Taras Shevchenko National University, Komlyk N. Yu.
Published: June 2021

This article is an attempt to a new look at the role of coordinate phrases in communication. The object of the research is English proverbs as representations of coordinate syntactic connection and the subject is coordinate phrases that are realized in them. The article presents a brief history of the study of coordinate phrases in English. The author made a comparative analysis of the views of domestic and foreign linguists on the problem of distinguishing coordinate phrases in the language. She distinguishes such differential signs of coordinate phrases in English: the equality of components, the uniformity of the syntactic function of all components, the integrity of the structure, the implementation of the connection between components with the help of coordinate conjunctions, the order of words and intonations. The expression of coordinate phrases with the adversative conjunction but and cumulative conjunction and in English proverbs was studied. Their semantic space and formal-grammatical features also were analyzed. The author revealed about 12 specific realizations of the semantic volume of coordinate phrases with the cumulative conjunction and 5 variants of opposing semantic-syntactic relations with the adversative conjunction but in paremias. After the analysis, the author concludes that the peculiarity of coordinate phrases with conjunction but is their relatively larger semantic and grammatical dependence on the whole sentence – proverb than in the coordinate phrases with and, which can function independently without a sentence, although in some cases their conceptual fullness is also realized only in a general judgment. Coordinate phrases, being carriers of a certain semantic volume, act as key concepts of proverbs.
Gaming Slang in the English Language
Authors:   National University ", Pavelieva A. K. ,   Yuri Kondratyuk Poltava Polytechnic", Lobko I. O. ,   National University "Yuri Kondratyuk Poltava Polytechnic"
Published: June 2021

The article deals with the peculiarities of gaming slang in the English language. Its goal is to describe gaming slang, to determine the ways of its formation, and to form the bases for the classifications of gaming slang. It also aims to analyze the problem of translating English gaming slang into Ukrainian, as the issue of gaming slang is not well-studied in linguistics. The connections of gaming slang with Internet, computer and more general youth slang are considered, mentioning their interrelations. The linguistic processes that are used in the formation of English gaming slang are determined, including the employment of metaphor and metonymy, the use of acronyms, abbreviations, clippings and portmanteau words. The following classifications are offered: classification by game genre, by number of players, and by topic of slang units. Every game genre brings its own features into the slang, and so does the number of players, since gaming slang is more actively used in multiplayer games. The authors suggest that the classification by topic should be developed for each genre separately, since the genre influences the gaming process and therefore generates special thematic categories of slang, such as words for the game mechanics, characters etc. The authors mention that English gaming slang is a significant translation problem, since this kind of slang is underdeveloped in the Ukrainian language.
The coagulopathy of liver disease: a shift in thinking
Authors: Ballantine Alexandra , Martin Daniel , Thakrar Sonali V
Published: June 2021

The coagulopathy of chronic liver disease causes derangement of the results of traditional laboratory tests. As such, there is an expectation that when undergoing invasive procedures patients with cirrhosis are at increased risk of bleeding. Standard practice is to optimise laboratory values with prophylactic transfusions of platelets, plasma and fibrinogen to reduce perceived bleeding risk. There has been a shift in thinking regarding coagulation in patients with chronic liver disease, whereby a rebalancing of haemostasis occurs with reduction in both procoagulants and anticoagulants. Guidelines for the preprocedural management of patients with chronic liver disease are inconsistent and may not account for this new paradigm. The risk of prophylactic transfusion should be measured against the risk of bleeding while considering the rebalancing of haemostasis. Future management may be guided by whole blood viscoelastic tests or use of thrombopoietin receptor agonists to optimise patients in these scenarios.
Ubuntu and the Perceptions of Unethical Leader Conduct: A Case Study of Public Secondary School Leaders in Kenya
Authors: Oduol Truphena
Published: June 2021

This paper extends and complements previous research on unethical leader behavior by examining the social and cultural perspectives that inform the understanding of objectional conduct among secondary school leaders in Kenya. The study used a social constructivist theoretical framework, and qualitative case study, and semi-structured interviews with school boards of governors, principals, and heads of department and school bursars. The findings revealed that cultural beliefs underpinned by the ubuntu ethic informed the school leaders' perceptions of unethical leadership behavior in the Kenyan secondary school contexts. Four sub-themes highlight acts that contradict the ubuntu values of altruism, humanness, care, and solidarity. They include disregard for community interests, neglect of care for one's kin, disregard for harmony, and elders' respect. The study concluded that western universal perspectives and definitions could not solely be relied upon to describe unethical leadership behavior in schools in non-Euro western contexts. The study contributes to the literature on unethical and ethical leadership by proposing a potential benefit in recognizing and incorporating non-western perspectives in exploring and defining the unethical leadership construct.
Real time measurement of intramuscular pH during routine knee arthroscopy using a tourniquet
Authors: MacDonald David R. W. , Neilly David W. , Elliott Kirsten E. , Johnstone Alan J.
Published: June 2021

Aims

Tourniquets have potential adverse effects including postoperative thigh pain, likely caused by their ischaemic and possible compressive effects. The aims of this preliminary study were to determine if it is possible to directly measure intramuscular pH in human subjects over time, and to measure the intramuscular pH changes resulting from tourniquet ischaemia in patients undergoing knee arthroscopy.

Methods

For patients undergoing short knee arthroscopic procedures, a sterile calibrated pH probe was inserted into the anterior fascial compartment of the leg after skin preparation, but before tourniquet inflation. The limb was elevated for three minutes prior to tourniquet inflation to 250 mmHg or 300 mmHg. Intramuscular pH was recorded at one-second intervals throughout the procedure and for 20 minutes following tourniquet deflation. Probe-related adverse events were recorded.

Results

A total of 27 patients were recruited to the study. Mean tourniquet time was 21 minutes (10 to 56). Tourniquet pressure was 300 mmHg for 21 patients and 250 mmHg for six patients. Mean muscle pH prior to tourniquet inflation was 6.80. Muscle pH decreased upon tourniquet inflation, with a steeper fall in the first ten minutes than for the rest of the procedure. Change in muscle pH was significant after five minutes of tourniquet ischaemia (p < 0.001). Mean muscle pH prior to tourniquet release was 6.58 and recovered to 6.75 within 20 minutes following release. No probe related adverse events were recorded.

Conclusion

It is possible to directly measure skeletal muscle pH in human subjects over time. Tourniquet ischaemia results in a decrease in human skeletal muscle pH over time during short procedures. Cite this article: Bone Joint Res 2021;10(6):363–369.
Can robotic technology mitigate the learning curve of total hip arthroplasty?
Authors: Kolodychuk Nicholas , Su Edwin , Alexiades Michael M. , Ren Renee , Ojard Connor , Waddell Bradford S.
Published: June 2021

Aims

Traditionally, acetabular component insertion during total hip arthroplasty (THA) is visually assisted in the posterior approach and fluoroscopically assisted in the anterior approach. The present study examined the accuracy of a new surgeon during anterior (NSA) and posterior (NSP) THA using robotic arm-assisted technology compared to two experienced surgeons using traditional methods.

Methods

Prospectively collected data was reviewed for 120 patients at two institutions. Data were collected on the first 30 anterior approach and the first 30 posterior approach surgeries performed by a newly graduated arthroplasty surgeon (all using robotic arm-assisted technology) and was compared to standard THA by an experienced anterior (SSA) and posterior surgeon (SSP). Acetabular component inclination, version, and leg length were calculated postoperatively and differences calculated based on postoperative film measurement.

Results

Demographic data were similar between groups with the exception of BMI being lower in the NSA group (27.98 vs 25.2; p = 0.005). Operating time and total time in operating room (TTOR) was lower in the SSA (p < 0.001) and TTOR was higher in the NSP group (p = 0.014). Planned versus postoperative leg length discrepancy were similar among both anterior and posterior surgeries (p > 0.104). Planned versus postoperative abduction and anteversion were similar among the NSA and SSA (p > 0.425), whereas planned versus postoperative abduction and anteversion were lower in the NSP (p < 0.001). Outliers > 10 mm from planned leg length were present in one case of the SSP and NSP, with none in the anterior groups. There were no outliers > 10° in anterior or posterior for abduction in all surgeons. The SSP had six outliers > 10° in anteversion while the NSP had none (p = 0.004); the SSA had no outliers for anteversion while the NSA had one (p = 0.500).

Conclusion

Robotic arm-assisted technology allowed a newly trained surgeon to produce similarly accurate results and outcomes as experienced surgeons in anterior and posterior hip arthroplasty. Cite this article: Bone Jt Open 2021;2(6):365–370.
Methodological Framework for the Quantification of GHG Emission Reductions from Climate Change Mitigation Actions
Authors: Sebos Ioannis , Progiou Athena G. , Kallinikos Leonidas
Published: June 2021

The quantification of the effect of climate change mitigation actions is avery useful exercise that can be used to meet a variety of objectives, such asinforming policy design, enhancing policy implementation, assessing policyeffectiveness, justifying budget allocation, and attracting climate finance. Itis also a reporting requirement according to the reporting framework of theUnited Nations Framework Convention on Climate Change (UNFCCC) andthe Monitoring Mechanism Regulation of the European Union (EU) aboutclimate change relevant information. However, the reporting of emissionssavings associated to mitigation actions by EU Member States is not com-plete, according to recent technical reports of the European EnvironmentalAgency. The purpose of this paper is to present a methodological frameworkthat can be used to quantify the effect (i.e. emission reductions) of themitigation actions. It is based on comprehensive and transparent models andformulas that could be easily tracked and reproduced. The proposed frame-work could be easily applied by EU Member States, but also by other non-EUcountries, to enhance the reporting of climate change related informationto European Commission and UN bodies. By applying the methodologicalframework for Greece, as a case study, it was possible to estimate the effectof implemented policies and measures for historical years (ex-post analysis),but also to estimate the projected effect (ex-ante analysis) of implemented,adopted and planned policies for future years (e.g. 2030).
Will China Suffer the Same Fate as the Soviet Union?
Authors: Martinez Carlos
Published: June 2021

It was widely assumed in the West following the collapse of European socialism that China would undergo a similar process of counter-revolution. This article seeks to understand why, three decades later, this hasn't happened, and whether it is likely to happen in the foreseeable future. The article contrasts China's “reform and opening up” process, pursued since 1978, with the “perestroika” and “glasnost” policies taken up in the Soviet Union under the Gorbachev leadership. A close analysis of the available data makes it clear that China's reform has been far more successful than the Soviet reform; that, in contrast to the Soviet Union in the 1980s, all the key quality of life indicators in China have undergone significant improvement in the last 40 years, and China is emerging as a global leader in science, technological innovation and environmental preservation. The article argues that the disparate outcomes in China and the Soviet Union are the result primarily of the far more effective economic strategy pursued by the Chinese government, along with the continued strengthening of the Communist Party of China's leadership.
Health System in Transition in India: Journey from State Provisioning to Privatization
Authors: Hooda Shailender Kumar
Published: June 2021

This paper highlights how privatization in healthcare is being promoted and its further growth facilitated through the adoption of neoliberal policies in India. The approach to financing healthcare has been shifting from public provisioning to tax-funded health insurance merely to achieve health coverage. The idea of the strategic purchasing of care from private providers promoted through insurance seems likely to aggravate the crisis in access and healthcare delivery. Such a crisis will escalate costs and promote concentration and monopolies in the healthcare market. Under the recently promoted neoliberal policy, India is compromising the goal of comprehensive provision of public health services, which is essential for creating a healthier society.
Ultrafast laser inscription of asymmetric integrated waveguide 3  dB couplers for astronomical K-band interferometry at the CHARA array
Authors: Benoît Aurélien , Pike Fraser A. , Sharma Tarun K. , MacLachlan David G. , Dinkelaker Aline N. , Nayak Abani S. , Madhav Kalaga , Roth Martin M. , Labadie Lucas , Pedretti Ettore , ten Brummelaar Theo A. , Scott Nic , du Foresto Vincent Coudé , Thomson Robert R.
Published: June 2021

We present the fabrication and characterization of 3 dB asymmetric directional couplers for the astronomical K-band at wavelengths between 2.0 and 2.4 µm. The couplers were fabricated in commercial Infrasil silica glass using an ultrafast laser operating at 1030 nm. After optimizing the fabrication parameters, the insertion losses of straight single-mode waveguides were measured to be

∼

1.2

±

0.5

d
B

across the full K-band. We investigate the development of asymmetric 3 dB directional couplers by varying the coupler interaction lengths and by varying the width of one of the waveguide cores to detune the propagation constants of the coupled modes. In this manner, we demonstrate that ultrafast laser inscription is capable of fabricating asymmetric 3 dB directional couplers for future applications in K-band stellar interferometry. Finally, we demonstrate that our couplers exhibit an interferometric fringe contrast of

>

90

%

. This technology paves the path for the development of a two-telescope K-band integrated optic beam combiner for interferometry to replace the existing beam combiner (MONA) in Jouvence of the Fiber Linked Unit for Recombination (JouFLU) at the Center for High Angular Resolution Astronomy (CHARA) telescope array.
Ultrawide photonic band gaps with the limit of gap-midgap ratio of 200% produced from complete-connected networks
Authors: Xu Xiaohui , Lu Jian , Yang Xiangbo
Published: June 2021

A kind of one-dimensional (1D) complete-connected network (CCN) is designed and its extraordinary optical property for producing an ultrawide photonic band gap (PBG) is investigated. The gap-midgap ratio formulaes of the largest PBGs created by CCNs are analytically derived, and the results indicate that with the increment of the node number in a unit cell, the number of the loops that can produce antiresonances increases fleetly, and consequently the gap-midgap ratio of the PBG produced by CCNs enlarges rapidly and tends rapidly to the limit at 200%. Moreover, the general transmission formula for 1D CCNs is analytically determined. Due to the periodicity, two types of transmission resonance peaks are generated, and the condition is analytically obtained from the transmission formula. This kind of CCN may have wide applications to design superwide band optical filters, optical devices with large PBGs and strong photonic attenuations, and other related optical communication and optical increment processing devices.
Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination
Authors: Opstad Ida S. , Hansen Daniel H. , Acuña Sebastian , Ströhl Florian , Priyadarshi Anish , Tinguely Jean-Claude , Dullo Firehun T. , Dalmo Roy A. , Seternes Tore , Ahluwalia Balpreet S. , Agarwal Krishna
Published: June 2021

Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique multiple signal classification algorithm (MUSICAL) does not assume stochastic independence of the emitter emission and can therefore exploit fluctuations arising from other sources, as such multimodal illumination patterns. In this work, we demonstrate and verify the utilization of fluctuations in the illumination for super-resolution imaging using MUSICAL on actin in salmon keratocytes. The resolution improvement was measured to be 2.2–3.6-fold compared to the corresponding conventional images.
Creation of Fano resonances and bound states in the continuum in metallic metasurface superlattices
Authors: Lee Sun-Goo , Kim Seong-Han , Kee Chul-Sik
Published: June 2021

A perfect metal film with a periodic arrangement of cut-through slits, an anisotropic metallic metamaterial film, mimics a dielectric slab and supports guided electromagnetic waves in the direction perpendicular to the slits. Since the guided Bloch modes exist only below the light line, conventional metallic metamaterial films do not exhibit interesting leaky-wave effects, such as bound states in the continuum and Fano resonances. Here, we introduce metallic metasurface superlattices that include multiple slits in a period and demonstrate that the superlattices support the Fano resonances and bound states in the continuum. We show that the number of Fano resonances and bound states depend on the number of slits in a period of superlattices through rigorous finite element method simulations. Experimental results in microwave region also support the creation of Fano resonance and bound states in the continuum by the increment of the number of slits in a period of superlattices.
13-W and 1000-Hz of a 2.7-µm laser on the 968 nm LD side-pumped Er:YAP crystal with concave end-faces
Authors: Quan Cong , Sun Dunlu , Zhang Huili , Luo Jianqiao , Hu Lunzhen , Han Zhiyuan , Dong Kunpeng , Chen Yuwei , Cheng Maojie
Published: June 2021

We demonstrate a 968-nm LD side-pumped Er:YAP laser with concave end-faces and a working frequency of 50∼1000 Hz. The maximum average powers of 26.75 and 13.18 W are obtained at 250 and 1000 Hz, corresponding to the slope efficiency of 16.2% and 9.0%, respectively. The M2 factors of 7.98 and 1.49 are determined under the multi-mode and single-mode, and three laser wavelengths of 2713, 2732 and 2796 nm are observed. The results indicate that the LD side-pumped Er:YAP crystal with negative curvature end-faces is a promising candidate for the high power and high frequency mid-infrared laser device.
Switchable terahertz metamaterial absorber with broadband absorption and multiband absorption
Authors: Zhang Man , Song Zhengyong
Published: June 2021

Based on the phase-transition property of vanadium dioxide (VO2), a terahertz bifunctional absorber is proposed with switchable functionalities of broadband absorption and multiband absorption. When VO2 is metal, the system is regarded as a broadband absorber, which is composed of VO2 patch, topas spacer, and VO2 film with metallic disks inserted. The system obtains a broadband absorption with absorptance >90% from 3.25 THz to 7.08 THz. Moreover, the designed broadband absorber has a stable performance within the incident angle range of 50°. When VO2 is dielectric, multiband absorption with six peaks is realized in the designed system. Graphene and the metallic disk-shaped array play the dominant role in the mechanism of multiband absorption. Through changing the Fermi energy level of graphene, the performance of multiband absorption can be dynamically adjusted. Because of the switchable functionalities, the proposed design may have potential application in the fields of intelligent absorption and terahertz switch.
Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800  cm-1
Authors: Hakert Hubertus , Eibl Matthias , Tillich Marie , Pries Ralph , Hüttmann Gereon , Brinkmann Ralf , Wollenberg Barbara , Bruchhage Karl-Ludwig , Karpf Sebastian , Huber Robert
Published: June 2021

Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from

1500

−

1800

c
m

−
1

. Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.
Non-line-of-sight optical camera communication aided by a pilot
Authors: Chen Shao-Qi , Chi Xue-Fen , Li Te-Yu
Published: June 2021

Currently, optical camera communication (OCC), as an emerging network access mode for the fifth-generation (5G), is valued for its advantages over radio frequency (RF). In the long-distance communication scenario, the data rate of line-of-sight (LOS) OCC is restricted. To address this, we propose a pilot-aided demodulation scheme to realize extending the communication distance, while also ensuring the data rate and reliability of the non-line-of-sight (NLOS) OCC. The experiment result validates the effectiveness of the proposed pilot-aided signal layer estimation (PSLE) and extreme value clustering (EVC) scheme, which can perform well against a heterogeneous background with ghost shadows at 2.5 m distance.
BIO-LGCA: A cellular automaton modelling class for analysing collective cell migration
Authors: Deutsch Andreas , Nava-Sedeño Josué Manik , Syga Simon , Hatzikirou Haralampos
Published: June 2021

Collective dynamics in multicellular systems such as biological organs and tissues plays a key role in biological development, regeneration, and pathological conditions. Collective tissue dynamics—understood as population behaviour arising from the interplay of the constituting discrete cells—can be studied with on- and off-lattice agent-based models. However, classical on-lattice agent-based models, also known as cellular automata, fail to replicate key aspects of collective migration, which is a central instance of collective behaviour in multicellular systems. To overcome drawbacks of classical on-lattice models, we introduce an on-lattice, agent-based modelling class for collective cell migration, which we call biological lattice-gas cellular automaton (BIO-LGCA). The BIO-LGCA is characterised by synchronous time updates, and the explicit consideration of individual cell velocities. While rules in classical cellular automata are typically chosen ad hoc, rules for cell-cell and cell-environment interactions in the BIO-LGCA can also be derived from experimental cell migration data or biophysical laws for individual cell migration. We introduce elementary BIO-LGCA models of fundamental cell interactions, which may be combined in a modular fashion to model complex multicellular phenomena. We exemplify the mathematical mean-field analysis of specific BIO-LGCA models, which allows to explain collective behaviour. The first example predicts the formation of clusters in adhesively interacting cells. The second example is based on a novel BIO-LGCA combining adhesive interactions and alignment. For this model, our analysis clarifies the nature of the recently discovered invasion plasticity of breast cancer cells in heterogeneous environments.
Designing microbial communities to maximize the thermodynamic driving force for the production of chemicals
Authors: Bekiaris Pavlos Stephanos , Klamt Steffen
Published: June 2021

Microbial communities have become a major research focus due to their importance for biogeochemical cycles, biomedicine and biotechnological applications. While some biotechnological applications, such as anaerobic digestion, make use of naturally arising microbial communities, the rational design of microbial consortia for bio-based production processes has recently gained much interest. One class of synthetic microbial consortia is based on specifically designed strains of one species. A common design principle for these consortia is based on division of labor, where the entire production pathway is divided between the different strains to reduce the metabolic burden caused by product synthesis. We first show that classical division of labor does not automatically reduce the metabolic burden when metabolic flux per biomass is analyzed. We then present ASTHERISC (Algorithmic Search of THERmodynamic advantages in Single-species Communities), a new computational approach for designing multi-strain communities of a single-species with the aim to divide a production pathway between different strains such that the thermodynamic driving force for product synthesis is maximized. ASTHERISC exploits the fact that compartmentalization of segments of a product pathway in different strains can circumvent thermodynamic bottlenecks arising when operation of one reaction requires a metabolite with high and operation of another reaction the same metabolite with low concentration. We implemented the ASTHERISC algorithm in a dedicated program package and applied it on E. coli core and genome-scale models with different settings, for example, regarding number of strains or demanded product yield. These calculations showed that, for each scenario, many target metabolites (products) exist where a multi-strain community can provide a thermodynamic advantage compared to a single strain solution. In some cases, a production with sufficiently high yield is thermodynamically only feasible with a community. In summary, the developed ASTHERISC approach provides a promising new principle for designing microbial communities for the bio-based production of chemicals.