bluuebunny/crossref_metadata_2025 huggingface dataset:
Accelerated development of hard high-entropy alloys with data-driven high-throughput experiments
Authors: Liu Yi , Wang Jiong , Xiao Bin , Shu Jintao
Published: March 2022

The development of multicomponent alloys with target properties poses a significant challenge, owing to the enormous number of potential component combinations, high costs and the inefficiency of conventional empirical trial-and-error experimental approaches. To tackle this challenge, we develop a machine learning (ML)-guided high-throughput experimental (HTE) approach to accelerate the development of non-equimolar hard CoxCryTizMouWv high-entropy alloys (HEAs). We first develop a set of all-process HTE facilities ranging from multi-tube ingredient assignment to multi-station electrical arc smelting and specimen preparation for bulk alloy samples with discrete compositions. Instead of random or combinatorial composition searching, HEAs with only ~1/28 of all the potential compositions are synthesized in two stages guided by the ML prediction. The final ML models, trained using 138 experimental data, predict the alloy hardness with mean relative errors of 5.3%, 6.3% and 15.4% at high (HV > 800), medium (HV = 600-800) and low (HV < 600) hardness ranges, respectively. In total, 14 superhard HEAs with HV > 900 are discovered by our ML-guided HTE approach. Moreover, the multiple ML models predict the hardness of 3876 hypothetical alloys covering the whole composition range, thereby revealing the systematic component effects based on the complete composition-hardness and descriptor-hardness correlations. The hardening mechanisms are elaborated by analyzing the microstructures of CoCrTiMoW. Furthermore, physical insights can be gained by transitioning from “machine learning” to “learning from machine”. This work demonstrates that our ML-guided HTE approach provides an effective strategy for multicomponent alloy development with a potential hundred-fold overall increase in efficiency at a fraction of the cost compared to conventional methods.
Application of Flexible Budget Based on Company Profit Model: Taking VK's Financial Data as an Example
Authors: Yao Yonghong , Li Jingjing , Yun Liu
Published: March 2022

There are many types of budgeting, and budgeting based on profit models is rare. This paper combines financial big data with management accounting methods to build a profit model in financial big data; and uses model data as the basis and basis for preparing flexible budgets, making budget preparation and execution more scientific and feasible; The company’s budget preparation method has been introduced; more importantly, financial accounting and management accounting are integrated, and the forecasting and budgeting functions of management accounting applications are really brought into play and applied.
Promoting Intelligent Finance, the Exploration and Innovation of Enterprise Financial Management System
Authors: Dong Ming , Ma Xinyan , Fan Lin
Published: March 2022

With the current changes in the trend of digital information technology, the development needs of the macroeconomic market within the new economic development stage and the international diversified economic wave, it is obvious that the financial management system of Chinese enterprises is no longer applicable.Then enterprise to promote intelligent finance, explore innovative enterprise financial management system for the urgent need. In view of the fact that, this article analyzes and discusses the problems belonging to the financial management system of Chinese enterprises at the existing stage, and creates a practicable path for the financial management system in a multi-dimensional and multi-level manner. The management of business operations and financial management is accompanied by a synchronised and optimized system for the management of communities, providing timely integration and dynamic quality enhancement.
Instantaneous Frequency Estimation Method for the Vibration Signal of Rotating Machinery Based on STFTSC Algorithm
Authors: Wu Binyun , Wang Shaojie , Hou Liang , Bu Xiangjian , Chen Chunhua , Yang Zheng
Published: March 2022

In this paper, an instantaneous frequency identification method known as STFTSC is developed by combining short-time Fourier transformation and the seam carving (SC) algorithm (widely used in image processing). In this method, the STFT is applied to analyze the time-frequency energy distribution of a vibration signal under variable-speed conditions. Subsequently, the energy gradient is calculated through the Sobel operator based on the time-frequency energy distribution. The energy gradient distribution contains multiple ridges, which are corresponding instantaneous frequencies of different orders. Finally, the targeted ridge extraction is transformed into an optimization problem, and the dynamic programming algorithm (DP) is used to search the targeted ridge with the minimum energy gradient to estimate the instantaneous frequency. The effectiveness of the proposed method is validated by a simulation experiment, moreover, a rotating machinery fault simulation test bench is employed to validate the method, which is then compared with polynomial chirplet transformation (PCT) and analyzed during the test process. The results show that the STFTSC instantaneous frequency estimation algorithm has a higher extraction accuracy and better application value in engineering problems than the PCT.
Biochemistry and Molecular Basis of Intracellular Flavonoid Transport in Plants
Authors: Pucker Boas , Selmar Dirk
Published: March 2022

Flavonoids are a biochemically diverse group of specialized metabolites in plants that are derived from phenylalanine. While the biosynthesis of the flavonoid aglycone is highly conserved across species and well characterized, numerous species-specific decoration steps and their relevance remained largely unexplored. The flavonoid biosynthesis takes place at the cytosolic site of the endoplasmatic reticulum (ER), but accumulation of various flavonoids was observed in the central vacuole. A universal explanation for the subcellular transport of flavonoids has eluded researchers for decades. Current knowledge suggests that a glutathione S-transferase-like protein (ligandin) protects anthocyanins and potentially proanthocyanidin precursors during the transport to the central vacuole. ABCC transporters and to a lower extend MATE transporters sequester anthocyanins into the vacuole. Glycosides of specific proanthocyanidin precursors are sequestered through MATE transporters. A P-ATPase in the tonoplast and potentially other proteins generate the proton gradient that is required for the MATE-mediated antiport. Vesicle-mediated transport of flavonoids from the ER to the vacuole is considered as an alternative or additional route.
SOFA Score Plus Impedance Ratio Predict Mortality in Critically Ill Patients Admitted to the Emergency Department: Retrospective Observational Study
Authors: Kammar-García Ashuin , Castillo-Martínez Lilia , Mancilla-Galindo Javier , Villanueva-Juárez José Luis , Pérez-Pérez Anayeli , Rocha-González Héctor Isaac , Arrieta Jesús , Remolina-Schlig Miguel , Hernández-Gilsoul Thierry
Published: March 2022

Background: The Sequential Organ Failure Assessment (SOFA) is a scoring system used for the evaluation of disease severity and prognosis of critically ill patients. The impedance ratio (Imp-R) is a novel mortality predictor. Aims: This study aimed to evaluate the combination of SOFA + Imp-R in the prediction of mortality in critically ill patients admitted to the emergency department (ED). Methods: A retrospective cohort study was performed in adult patients with acute illness admitted to the ED of a tertiary-care referral center. Baseline SOFA score and bioelectrical impedance analysis to obtain the Imp-R were performed within the first 24 hours after admission to the ED. A Cox regression analysis was performed to evaluate mortality risk of initial SOFA score plus Imp-R. Harrell's C-statistic and decision curve analyses (DCA) were performed. Results: Out of 325 patients, 240 were included for analysis. Overall mortality was 31.3%. Only 21.3% of non-surviving patients died after hospital discharge, and 78.4% died during hospital stay. Of the latter, 40.6% died in the ED. SOFA and Imp-R values were higher in non-survivors and were significantly associated with mortality in all models. The combination of SOFA + Imp-R significantly predicted 30-day mortality, in-hospital mortality, and ED mortality with area under the curve (AUC) of 0.80 (95% CI: 74-0.86), 0.79 (95% CI: 0.74-0.86) and 0.75 (95% CI: 0.66-0.84) respectively. The DCA showed that combining SOFA + Imp-R improved the prediction of mortality through the lower risk thresholds. Conclusion: The addition of Imp-R to baseline SOFA score at admission to the ED improves mortality prediction in severely acutely ill patients admitted to the ED.
Performance Evaluation of Integrated Public Security Areas: Application of the PROMETHEE II Method in the Brazilian Context
Authors: Basilio Marcio , Pereira Vladecy , Costa Helder
Published: March 2022

Purpose: This research aims to model a police agency ordering problem based on criteria that measure the performance of operational and logistical variables, using a multicriteria method. Design/methodology/approach: The multicriteria method PROMETHEE II was used, which with the aid of the Visual PROMETHEE software, emulated the systematized data in the impact matrix and produced the final ordering of the most efficient police agencies in equalizing the operational, logistical, financial, and human resources applied in the combating crime and reducing criminal indices. Findings: The results confirmed that the resources made available to police agencies located in different municipalities in the metropolitan region of the state of Rio de Janeiro are better applied by managers compared to police units in the capital of Rio de Janeiro. Research limitations/implications: As implications of the research, it can be inferred that the use of multicriteria methods in modelling problems in ​​public security can contribute to the rationalization of the use of available resources to fight crime in large cities. Research has shown that it is possible to use the multicriteria methodology in ordering police agencies that best equalize the available resources. Practical implications: The practical impact of this research lies in optimizing the resources available to law enforcement agencies in the fight against crime in general. Social implications: The results can influence the decision making of the local government in the allocation of resources, as well as offering sectors of the economy information relevant to local development. Originality/value: The results of the MCDA analysis can be used to help police agencies in Rio de Janeiro, Brazil to be more efficient. In addition, the application of MCDA can be a new approach to measuring the efficiency of police services.
On the Essential Role of SARS-CoV-2 Localization in the Nasopharynx and Intestines in the Pathogenesis of COVID-19
Authors: Afonyushkin Vasily , Akberdin Ilya , Kozlova Yulia , Shchukin Ivan , Mironova Tatyana , Bobikova Anna , Donchenko Nikolay , Cherepushkina Viktoria , Poletaeva Yulia , Kolpakov Fedor
Published: March 2022

Patients with COVID-19 may develop pneumonia, severe symptoms of acute respiratory distress syndrome, and multiple organ failure. Nevertheless, the variety of forms of this disease, requires further research on the pathogenesis of this disease. Based on the analysis of published data on the concentrations of SARS-CoV-2 in biological fluids of the nasopharynx, lungs and intestines and using a developed modular model of the virus distribution in human tissue and organs, an assessment of the SARS-CoV-2 reproduction in various compartments of the body is presented. Most of viral particles can enter into the esophagus from nasopharynx. Entering viral particles into the gastrointestinal tract will obviously be accompanied by infection of the intestinal epithelium and accumulation of the virus in the intestinal lumen in an amount proportional their secretory and protein-synthetic activities. The relatively low concentration of SARS-CoV-2 in tissues implies an essential role of transport processes and redistribution of the virus from nasopharynx and intestines to lungs. The model simulations also supposes that sanitation of the nasopharynx mucosa at the initial stage of the infectious process considering inhibition of the virus accumulation by means of cellular and humoral responses has prospects for the use in medicine practice.
Synthesis and Spectroscopic Studies of Novel Dichloro and Polymethoxy P-Nitrophenyl Hydrazones: A Comparative Study
Authors: Babalola Sodeeq , Igie Nosakhare , Idris Abullah , Sanni Yeken , Hamza Asmau , Muhammad Hayatudeen , Erumiseli Oshoma , Bakare Lateef
Published: March 2022

We conducted a comparative study on the development of two synthetic methods. The solvent-free synthesis is a green chemistry method developed in a bid to ameliorate environmental adverse effects of the conventional solvent-based synthesis. The synthesis of novel dichloro and polymethoxy p-nitrophenylhydrazones through solvent free technique gave moderate to high yields which were however lower than those of the solvent-based method. The established solvent-free approach has several benefits, including universality and simplicity of the approach, catalyst-free conditions, non-use of an organic solvent, quick reaction time, fast and efficient workup, and un-solvated pure products in moderate to high yields.
Early Life Exercise Training and Inhibition of Apolipoprotein B Expression to Improve Age-Related Arrhythmias and Prolong the Average Lifespan in Drosophila
Authors: Ding Meng , Li Qiufang , Peng Tianhang , Wang Tongquan , Yan Hanhui , Tang Chao , Wang Xiaoya , Guo Yin , Zheng Lan
Published: March 2022

Cardiovascular disease (CVD) places a heavy burden on older patients and the global healthcare system. A large body of evidence suggests that exercise training is essential in preventing and treating cardiovascular disease, but the underlying mechanisms are not well understood. Here, we used the Drosophila melanogaster animal model to study the effects of early-life exercise training (ELET) on the aging heart and lifespan. We found in flies that age-induced arrhythmias are conserved across different genetic backgrounds. The fat body is the primary source of circulating lipoproteins in flies. Inhibition of fat body apoLpp (the flies apoB homolog) demonstrated that low expression of apoLpp reduced the development of arrhythmias in aged flies but did not affect average lifespan. At the same time, ELET can also reduce the expression of apoLpp mRNA in aged flies and have a protective effect on the heart, which is similar to the inhibition of apoLpp mRNA. Although treatment of apoLppRNAi and ELET alone had no significant effect on lifespan, the combination of apoLppRNAi and ELET extended the average lifespan of flies. Therefore, we conclude that apoLppRNAi and ELET are sufficient to resist age-induced arrhythmias, which may be related to the decreased expression of apoLpp mRNA, and that apoLppRNAi and ELET have a combined effect on prolonging the average lifespan.
Динамическая балансировка с помощью пакета параллельной декомпозиции GridSpiderPar
Authors: Головченко Евдокия Николаевна , Golovchenko Evdokiya Nikolaevna
Published: March 2022

При проведении параллельных расчетов часто используются динамические адаптивные сетки. В процессе вычислений сетка измельчается или огрубляется в зонах интереса или в местах возникновения больших градиентов целевых функций. Для балансировки нагрузки процессоров требуется еe периодическое переразбиение. На основе параллельного алгоритма геометрической декомпозиции и параллельного инкрементного алгоритма декомпозиции графов пакета GridSpiderPar были разработаны алгоритмы динамической балансировки нагрузки процессоров. Выполнено сравнение существующего разбиения гексаэдральной сетки с локальным измельчением (6.7$\cdot$10$^6$ ячеек) и результатов балансировки разработанными алгоритмами декомпозиции. Результаты показали преимущества параллельного алгоритма геометрической декомпозиции на данной сетке и особенности применения параллельного инкрементного алгоритма.
Diminished complexity of heart rate time series in adolescents facing negative events during everyday life
Authors: Bornas Xavier
Published: March 2022

Physiological systems need to be flexible in order to adapt to a changing environment. Negative events, however, induce flexibility reductions that seem necessary for coping purposes. To date, studies have measured linear variability and entropy in heart output, but none have examined the scaling properties of the cardiac system when individuals deal with stressful everyday events. This study was aimed at testing the hypothesis that the complexity of the cardiac dynamics is diminished when individuals face negative events in real life. Cardiac variability (linear) and complexity (nonlinear), as well as discomfort and effectiveness of event-related emotion regulation (EER) were ecologically examined in N = 65 adolescents (Mage = 14.80 years; SDage = 0.86; 55.38% girls). Repeated Measures MANOVAs revealed higher heart rate (HR) and lower cardiac complexity (higher long-term scaling exponent, p = .029; lower Fractal Dimension FD, p = .030; and lower Sample Entropy, p = .001) during EER in comparison with non-emotion regulation conditions (NER). Wilcoxon non-parametric tests revealed higher Hurst exponents (p = .006) in EER than in NER. No significant correlations were found between discomfort and cardiac variables although the higher the cardiac entropy in NER conditions, the greater the self-rated effectiveness of EER (p < .050). EER processes involved increases in HR as well as scaling and FD changes that might reflect the real-time scale’s predominance in HR output when adolescents are dealing with negative events. Keywords: adolescence; emotion regulation; cardiac complexity; heart rate variability; observational descriptive study
Propiedades Psicométricas del Cuestionario de Regulación Emocional (ERQ-CA) en Estudiantes Universitarios Colombianos
Authors: Alfonso Gutiérrez Laura Sofía
Published: March 2022

El propósito de esta investigación fue analizar las propiedades psicométricas del cuestionario de regulación emocional (ERQ-CA) en población colombiana. Se utilizó una muestra de 798 estudiantes universitarios de Bogotá y sus alrededores, con edades entre 15 y 25 años (M = 19.59, DE = 1.78). La muestra se dividió en dos grupos proporcionales. La primera parte de la muestra estuvo conformada por el 25% (n = 200). Con ella se realizó el análisis factorial exploratorio, χ² (13) = 16.38, p < 0.23, encontrando una estructura bidimensional, de acuerdo con los índices de bondad de ajuste: TLI = .951, RMSEA = .057, SRMR = .04. Con la segunda submuestra (n = 598) se realizó el análisis factorial confirmatorio. Se encontraron excelentes índices de ajuste: S-Bχ² (20) = 45.812; χ²/gl = 2.29; CFI = .962; NNFI = .940; TLI = .947; IFI= .958; RMSEA = .051; SRMR = .048. Se obtuvieron las estimaciones y errores del modelo a través de métodos robustos, se realizó el análisis de consistencia interna, se estudió la invarianza del modelo por género y se hallaron las diferencias por género. Se puede concluir que el ERQ-CA es un cuestionario con propiedades psicométricas muy favorables para su uso en el contexto colombiano. Palabras clave: Regulación emocional; Control emocional; Medición; Análisis factorial; Psicometría.
Diagnostic value of a new cryoprobe for peripheral pulmonary lesions: a prospective study
Authors: Tanaka Midori , Matsumoto Yuji , Imabayashi Tatsuya , Kawahara Takuya , Tsuchida Takaaki
Published: March 2022

Background: Cryobiopsy is an established technique that yields larger and higher-quality samples than does a forceps biopsy. However, it remains underutilised in the diagnosis of peripheral pulmonary lesions (PPLs), mainly because of difficulties in handling conventional cryoprobes. A recently introduced single-use cryoprobe with a smaller diameter and more flexibility than conventional ones may improve its diagnostic ability for PPLs. We conducted this prospective study to evaluate the feasibility of transbronchial cryobiopsy in the diagnoses of PPLs, using a new 1.7-mm cryoprobe. Methods: The study included patients with PPLs less than 30 mm in diameter scheduled to undergo bronchoscopy. All the procedures were performed using a combination of virtual bronchoscopic navigation, radial endobronchial ultrasound (R-EBUS) and X-ray fluoroscopy, and all the samples were collected using the cryoprobe alone. Thereafter, we assessed the diagnostic outcomes and safety profiles.Results: A total of 50 patients were enrolled and underwent cryobiopsy. The median lesion size was 20.8 mm (range, 8.2–29.6 mm), and the negative bronchus sign was seen in 34% of lesions. The diagnostic yield was 94% (95% confidence interval, 83.5–98.8%). A positive bronchus sign had a significantly higher diagnostic yield than did a negative bronchus sign (100% vs. 82.4%; P=0.035). The yield was achieved regardless of other variables, including lesion size, location, and R-EBUS findings. The major complications were mild and moderate bleeding in 28% and 62% of patients, respectively. Pneumothorax was identified in one patient.Conclusion: Transbronchial cryobiopsy using the new 1.7-mm cryoprobe is a feasible procedure that has the potential to increase the diagnostic accuracy for PPLs.Trial Registration: Japan Registry of Clinical Trials, jRCT1032200065. Registered July 8 2020, https://jrct.niph.go.jp/en-latest-detail/jRCT1032200065
The use of novel liquid mulching formulations (hydromulching) increases yield and quality of artichoke (Cynara cardunculus var. scolymus) by improving soil physicochemical and biological properties
Authors: Romero-Muñoz Miriam , Gálvez Amparo , Amor Francisco M. del , Albacete Alfonso , López-Marín Josefa
Published: March 2022

Purpose: We hypothesized that organic liquid mulching formulations called hydromulching can be used in sustainable agricultural production through its direct influence on soil physicochemical and biological properties to improve crop yield and quality. Methods: Artichoke plants were grown using three hydromulching treatments, rice hulls (RH), wheat straw (WS), and mushroom substrate (MS), and two control treatments, soil covered with polyethylene (PE) and bare soil (BS). Soil quality-related parameters and artichoke head physical and chemical properties were measured for each mulching treatment.Results: Soil organic matter and soil organic carbon increased in all hydromulching treatments, especially with the RH cover. Soil moisture and the contents of K+, P, Cu2+, Mn2+, and Zn2+ enhanced in hydromulched soils, particularly in MS-covered soil. The WS treatment produced the highest crop yield, while the lowest yield was obtained in bare soil. Importantly, plant water relationships were superior in all mulching treatments giving rise to the improvement of crop yield and the most important physical quality parameters in artichoke, namely color, firmness, and size. Furthermore, the content of some important nutrient elements in artichoke (K+ and N) were greater in RH and MS treatments, while sugar concentrations were higher in bare soil with respect to the other treatments. Conclusion: Hydromulching is a sustainable alternative to plastic mulching through the regulation of plant hydric status and soil moisture and fertility, thus improving yield and physical quality characteristics of artichoke heads, and maintaining their chemical and nutritional quality over bare soil.
Mechanism of novel enoxaparin sodium high viscosity bone cement reducing thrombosis by regulating the expression of CD40 in endothelial cells
Authors: Sang Linchao , Hao Kangning , Ding luobin , Shen Xiaoyu , Fu Dehao , Qi Xiangbei , Sun Hui
Published: March 2022

Objective: PMMA bone cement develop local thrombi, to solve that,our study found that ES-PMMA bone cement,a new kind of material,could reduce local thrombosis.We took a simple and reproducible animal model to confirm the reducing and preliminarily explored the molecular mechanism. Methods: On the basis of carotid artery vein extracorporeal shunt, the New Zealand rabbits were modeled into three groups: Sham group, PMMA group and ES-PMMS group. There were 10 rabbits in each group. Four hours after modeling,collecting the experimental samples,and comparing the amount of thrombosis between those groups. The collected vascular tissue samples were quantitatively detected for endothelial cell related thrombomodulin.Results: There were thrombosis in PMMA group and ES-PMMA grounp,which didn't exist in Sham group. The weight of thrombosis in PMMA group was 0.00732±0.00089g/cm, The weight of thrombosis in the ES-PMMA group was 0.00554±0.00077g/cm，P < 0.001. We found that the expression of CD40, a related protein that can regulate thrombosis in vascular endothelial cells, was significantly lower in the ES-PMMA group than that in the PMMA group using RT-qPCR as well as Western blot.Conclusion: Compared with PMMA bone cement, ES-PMMA bone cement can reduce local thrombosis by decreasing the expression of thrombus associated regulatory protein CD40 in vascular endothelial cells.
Early-stage colon cancer with high MALAT1 expression is associated with the 5-Fluorouracil resistance and future metastasis
Authors: Aksoy Secil Ak , Berrin Tunca , Tezcan Gulcin , Ozturk Ersin , Cecener Gulsah , UGRAS Nesrin , Yilmazlar Tuncay , YERCI Omer
Published: March 2022

Background: This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC).Methods and Results: The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages.Results: High MALAT1 (p=0.0002) and low PTENP1 (p= 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p=0.0009) was increased while the expression of PTENP1 (p=0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells.Conclusion: MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance.
Does the transition to renewable energy increase inflation in European countries?
Authors: ARSLAN Ünal , Yıldız Tuba
Published: March 2022

In European countries, because of the expensiveness of the technology required for the use of renewable energy sources, it can cause inflation by constantly increasing prices due to cost increases in the short term. However, in the long term, costs can be reduced by providing the necessary technological infrastructure, saving energy thanks to this technological development, eliminating harmful effects on the environment. In this way, renewable energy sources have a long-term effect on inflation. This study aims to examine the long-term relationship between renewable energy and inflation in European Union countries. It is thought that this study will contribute to the literature, since there are no studies that examine the relationship between renewable energy and inflation, among the national and international studies, which deal with the European Union countries and examine the PMG and CCE co-integration analyses. According to the results of the PMG cointegration analysis, while there is no significant relationship between inflation and renewable energy in the short term, renewable energy affects inflation negatively in the long term. According to the results of the CCE cointegration analysis, in the long run, renewable energy affects inflation negatively in Sweden, the United States, and Luxembourg.
Cadmium Affect Root Growth in Japonica Rice (Oryza sativa L.) Negatively Interacting with JA at Bud Stage
Authors: Wu Bohan , Zeng Zifan , Wu Xiangyang , Li Yingying , Wang Fangquan , Yang Jie , Li Xia
Published: March 2022

To explore the role of jasmonic acid in seed germination of rice under Cd stress, the growth indices of JJ818 and NJ9108 under the bud stage were measured for identifying study the molecular mechanism of variety difference in cadmium tolerance. The tolerant variety JJ818 and the sensitive variety NJ9108 were treated under 150 µmol·L− 1 Cd concentration, methyl jasmonate (MeJA), and Ibuprofen (IBU) alone or in combination. The germination rate, root length, bud length, soluble protein content, soluble sugar content, and sugar components, α-amylase activity, and its related gene expression were measured. The results showed that the root length of the two rice materials were significantly inhibited during germination under the Cd treatment, however, the germination rate and shoot length had little affected. MeJA decreased the germination rate, root length and shoot length, and the contents of soluble sugar and sugar components and α-amylase activity as well. However, IBU treatment showed the opposite trend. Cd treatment also decreased the expression of the αAmy and RL1, and the accompanying changes of up-regulated the gene expression of JIOsPR10, OsLOX1, and OsLOX4 related to synthesizing jasmonic acid(JA). The results showed that JA negatively regulates the cadmium of rice and the genes related to the synthesis and response of JA were related to the variety difference in cadmium tolerance at the bud stage. Compared with the NJ9108, JJ818 was insensitive to jasmonic acid.
Microenvironment-related gene IGLL5 has potential to predict the prognosis of clear cell renal cell carcinoma
Authors: Bu Ning , Dan Weichao , Li Wenfei , Kong Zhidong , Gao Wei , Miao Yi
Published: March 2022

Background: Clear cell renal cell carcinoma (ccRCC) is a common malignancy of the urinary system. With the increasing use of immune checkpoint inhibitors, the key roles of the tumour microenvironment (TME) in tumogenesis, tumour development and treatment resistance are gradually recognised. However, the understanding of ccRCC-TME and progression remains unclear.Methods: Transcriptome RNA-seq data and their corresponding clinical data were obtained from The Cancer Genome Atlas. Differentially expressed genes (DEGs) were identified by estimating stromal and immune scores. Common DEGs were identified by protein–protein interaction (PPI) network and univariate Cox regression analyses. Furthermore, Western blot was used to verify the expression level of candidate biomarkers in ccRCC cells. In addition, gene set enrichment analysis and immune infiltration analysis were performed to explore the mechanism of ccRCC and related immune cells. Finally, the correlation between the core genes and clinicopathological characteristics was assessed for potential clinical application.Results: IGLL5 was the only core gene found through PPI network intersection analysis and Cox regression analysis. IGLL5 was negatively correlated with the prognosis of patients with RCC. The IGLL5 protein expression was elevated in RCC. Clinically, 453 (86.45%) and 71 (13.55%) patients with RCC were stratified into the IGLL5≤16.33 and IGLL5>16.33 groups, respectively.Compared with IGLL5≤16.33, the IGLL5>16.33 group conferred a higher risk of developing high stage (odds ratio [OR], 2.759; 95% confidence interval [CI], 1.651–4.609) and high T stage (OR, 2.686; 95% CI, 1.615–4.467; all P<0.001).Conclusion: IGLL5 gene is a potential predictor of the high malignancy of RCC. It may serve as a potential immunotherapeutic target for RCC.
Clinicopathological features and incidence rate of oxyntic gland neoplasm: A single center retrospective study
Authors: Takao Toshitatsu , Asahara Hikari , Asahara Yumiko , Asahara Masakyo , Sakaguchi Hiroya , Yoshizaki Tetsuya , Ikezawa Nobuaki , Takao Madoka , Morita Yoshinori , Toyonaga Takashi , Kushima Ryoji , Kodama Yuzo
Published: March 2022

In this retrospective study, we aimed to examine oxyntic gland neoplasms (OGNs) identified in patients visiting an internal medicine clinic over a 40-month follow-up period. Additionally, we wished to clarify the clinicopathological characteristics and frequency of occurrence of these lesions. From December 1, 2017 to March 31, 2021, we performed 13,240 upper gastrointestinal endoscopies on 7488 patients. Of these, we identified 27 patients with 30 histopathologically confirmed OGNs, yielding a disease frequency of 0.36% (27/7488). Further, in three of 27 patients (11%), multiple simultaneous lesions had occurred. One of the 30 lesions (3.3%) was present in the antrum. Nine of the 9 patients (33%) had no history of Helicobacter pylori infection, and remaining 18 (67%) were either currently or previously infected. However, 27/30 lesions (90%) occurred in non-atrophied regions. After endoscopic treatment, submucosal infiltration was observed in eight (47%) of the 17 lesions that underwent histopathological evaluations, which showed no submucosal desmoplasia in all eight cases. Additional resection was not performed while patient progress has been monitored. No cases of recurrence have been reported. In conclusion, we found that the frequency of oxyntic gland neoplasms was much higher than previously reported.
Genetic characterization of a novel HIV-1 CRF01_AE/ CRF07_BC recombinant form among men who have sex with men in Baoding City, Hebei Province, China
Authors: Yang Xuegang , Zhu Huiying , An Weina , Zhao Jing , Lu Xinli , Sun Wenlong , Li Yongqin
Published: March 2022

Nowadays, a large number of unique recombinant forms have been found among sexual transmission populations in China. This study reported a novel HIV-1 second-generation unique recombinant form (URF), which was isolated from an HIV-positive man named BD201AJ who was infected through men who have sex with men(MSM) in Baoding City, Hebei province, China. This is different from a CRF01_AE/CRF07_BC recombinant strain from Hebei province, in China. The analysis result of the near full length genomic characterization showed that the genome comprises at least twelve interlaced segments, including six CRF07_BC and six CRF01_AE segments, with CRF07_BC as the main framework. No similar breakpoints between our strain and the other strains in the Los Alamos HIV database were observed. This highlights the importance of monitoring HIV-1 molecular epidemiological characteristics and the ongoing generation of recombinant viruses may provide an important insight for future analysis the recombination mechanism of HIV-1.
An In Vivo Definition of Brain Histamine Dynamics Reveals Critical Neuromodulatory Roles for This Elusive Messenger
Authors: Berger Shane , Baumberger Beatrice , Samaranayake S , Hersey M , Mena Sergio , Bain Ian , Duncan W , Reed M C , Nijhout F , Best J , Hashemi Parastoo
Published: March 2022

Histamine is well known for mediating peripheral inflammation, but histamine is also found in high concentrations in the brain where amongst other roles, this amine is thought to be neuromodulator. Neuromodulation is critical for brain function, yet histamine dynamics are very difficult to measure and thus several fundamental aspects of the mechanisms that control the extracellular and modulatory behavior of this messenger remain undefined. In this work we undertake the first in-depth characterization of in vivo histamine dynamics in real time using fast-scan cyclic voltammetry at carbon fiber microelectrodes. We measure electrically evoked histamine in the mouse hypothalamus and find that histamine release is sensitive to pharmacological manipulation at the level of synthesis, packaging, autoreceptor control of release, and metabolism. We find two breakthrough aspects of histamine modulation. First, there are differences in H3 receptor regulation of histamine between sexes showing that histamine release in female mice is more much tightly regulated than in male mice under H3 or inflammatory drug challenge. We hypothesize that this finding may contribute to hormone-mediated neuroprotection mechanisms in female mice. Second, we find that a high dose of a commonly available antihistamine, the H1 receptor inverse agonist diphenhydramine, rapidly decreases serotonin levels. This high dose is considered overdose; however, this finding highlights the sheer significance of better consideration of the modulatory nuances of histamine on serotonin.We, thus, present the first in depth in vivo characterization of fast histamine dynamics and highlight two breakthrough regulatory aspects of this elusive modulator. The implications of our study are new avenues to better understand and treat histamine related disorders of the brain (such as neuroinflammation), emphasizing that sex and modulation (of serotonin) are critical factors to consider when studying/designing new histamine targeting therapeutics.
An unusual location of an epidermoid cyst, the middle phalanx of the middle finger: A case report
Authors: (MS MRCS) *Dr. Dheeraj Makkar , (MS) Prof. Ravi Sahuta
Published: March 2022

Introduction:Epidermoid cysts are cutaneous cysts frequently observed among farmers, factory workers, carpenters, and tailors due to their occupations' predilection for repetitive minor hand trauma.Case Presentation:A 66-year-old male laborer presented to our hospital with a swelling on the palmar aspect of his right middle finger. The swelling was accompanied by pain and an inability to work over the previous four months. A 2.5 cm × 2.5 cm smooth, tender, immobile, non-compressible hard swelling was detected just above the middle phalanx during the clinical examination on the volar aspect of the right middle finger. The diagnosis was confirmed by histopathology after excision. The patient's range of motion improved significantly following surgery, and his pain subsided.Conclusion:We present a novel location for an epidermal inclusion cyst and the importance of histopathologic evaluation in this case. Additionally, we emphasize the significance of extensively checking the cyst's epithelium to guarantee complete capsule removal and rule out any additional pathology.
A Novel Model of Inducing Apoptosis of Mouse Cardiac Myocytes by Thermal Stimulation
Authors: Yang Jia , Sun Qihui , Ma Qingyun , Yu Qinhui , Liu Yanliang , Han Yuxiu , Xing Shuyan , Rong Rong , Yang Yong
Published: March 2022

Background: Thermal stimulation is a potent inducer of apoptosis in many cell lines. At present, water bath heating is often used to thermal stimulation-induced apoptosis, but this method has poor reproducibility and low efficiency. The aim of this study was to build a simple and reproducible apoptosis model caused by thermal stimulation. Methods and Results: The apoptosis and necrosis rate were detected under different thermal stimulation conditions by flow cytometer. We also analyzed the difference of growth curves between the thermal stimulation model group and the normal group. According to the cell growth curve, the cell re-culture time after thermal stimulation was preliminarily detected, and the reasonability of the time was verified by flow cytometer. The conditions for establishing the model are: PCR calculated temperature control system with a gradual heating pattern, 37°C, 2 min; 38°C, 2 min; 39°C, 2 min; 40°C, 2 min; 41°C, 2 min; 42°C, 2 min; 43°C, 60 min; 42°C, 2 min; 41°C, 2 min; 40°C, 2 min; 39°C, 2 min; 38°C, 2 min; 37°C, 2 min. In this way, the proportion of cell apoptosis is higher than that of cell necrosis with satisfied reproducibility. Conclusions: The present work provide a new method to induce cell apoptosis by thermal stimulation.
Molecular Viewpoint for the Stability Mechanism of SiO2/SDS Dispersion
Authors: Liu Nannan , Chen Yan , Chen Xinglong , Du Hui , Xu Hui , Zhang Yingnan , Shao Minglu , Zhao Huijun , He Yanfeng , Ju Binshan , Sun Zheng
Published: March 2022

The dispersion of silica dioxide (SiO2)/sodium lauryl sulfate (SDS) have been widely used in the oilfield development and the remaining oil displacement, but its instability is still a problem in the practical engineering application. In this paper, the mechanism of foam stability was investigated by combining the measurement of interfacial energy of nanoparticles at the gas-liquid interface with the dynamics simulation of molecular diffusion. The results showed that the addition of SiO2 nanoparticles improved the interfacial energy and interfacial activity at the gas-liquid interface, meanwhile limited the movement of SDS molecules and water molecules, which was beneficial for the foam stability. Notably, the addition of modified SiO2 nanoparticles further enhanced the interfacial energy at the gas-liquid interface and strengthened the restriction of water/SDS molecular movement, thereby slowing down the drainage and decay of the foam dispersions. The mechanism investigation was of benefit for foam flooding engineering application.
Diabetes exacerbated sepsis-induced intestinal injury by promoting M1 Macrophage Polarization via miR-3061/Snail1 signaling
Authors: Tan Fang , Cao Yuling , Zheng Lei , Wang Tao , Zhao Shuhua , Chen Jiong , Pang Changji , Xia Zhengyuan , Li Ningning , Chi Xinjin
Published: March 2022

Background
Macrophages play the important roles in the diabetes and sepsis-related intestinal injury. Accumulating evidence suggests that transcription factors act as the fundamental link between macrophage polarization and tissue injury. However, the underlying mechanisms of transcription factors regulating macrophage polarization-related intestinal injury remain unclear under diabetes and sepsis conditions.
Methods
The cecal ligation and puncture (CLP)-induced sepsis models were established in both wild type (WT) and diabetic male mice. Clodronate liposome (Cls) was used to deplete macrophage. HE staining, inflammatory cytokines (TNF-α, IL-1β and IL-6) and intestinal mucosal barrier function markers (occludin, ZO-1, LPS and iFABP) were used to elevated intestinal damage. MicroRNA-array, RNA-seq and bioinformatic analysis were performed to detect the microRNA and mRNA expression and the potential regulation mechanism. In vitro, high glucose and LPS, miR-3061 mimics and Snail siRNA stimulation of RAW264.7 cells were cultured for further mechanism studies. Luciferase reporter assay was used to confirm the interplay between microRNA and its target genes.
Results
Compared to WT CLP mice, the diabetic CLP mice showed severe intestinal damage characterized by significantly increased Chui’s scores, expression of inflammatory cytokines (TNF-α, IL-1β and IL-6), serum LPS and iFABP concentration and reduced tight junction protein occluding and ZO-1 levels. Macrophage deplettion exhibited reverse the intestinal damage caused by CLP. The bioinformatic analysis identified that miR-3061/Snail1 might be a potential regulation axis of macrophage polarization. Furthermore, high glucose and LPS stimulation increased M1 macrophage and reduced levels of miR-3061, which was negatively associated with Snail1 in RAW264.7 cells. Mechanistic researches demonstrated that miR-3061 regulated macrophage polarization by targeting Snail1 mRNA 3′-untranslated region (3’‐UTR). Moreover, miR-3061 overexpression suppressed Snail1 expression, inhibited M1 macrophage and inflammatory cytokines.
Conclusion
This study elucidated that diabetes exacerbated sepsis-induced intestinal injury by promoting M1 macrophage polarization, and further demonstrated that the miR-3061/Sani1 axis may be the potential target of macrophage polarization.
The Effect of Variation in Dietary Cation-Anion Difference During the Transition Period of Brown Swiss Dairy Cows on Calcium Status, Blood Metabolites and Rumen Activity
Authors: Hassanien H. E. M. , Abdel-Raouf E. M. , Mahmoud A. M. M.
Published: March 2022

The objective of this study was to determine the effects of varying pre- and postpartum DCAD diets on serum total calcium, ionized calcium, blood and ruminal fluid metabolites, and milk production in prepartum and postpartum Holstein cows. Fifty-four multiparous dry Brown Swiss cows n = 54, were enrolled in a completely randomized block experimental design at 29 days prior to expected parturition through 86 days in milk. A 3 x 2 factorial arrangement of treatments was utilized. Three DCAD levels were fed precalving (0, -120 and − 200 mEq/kg DM, n = 18 cows per treatment) and two DCAD levels were fed post calving (+ 200 and + 400 mEq/kg DM, n = 27 cows per treatment). Prepartum urine pH was lower for cows fed − 200 DCAD compared with those fed − 120 or 0 DCAD. Postpartum urine pH was higher for cows fed + 400 mEq/kg compared to cows fed + 200 mEq/kg DCAD. Prepartum serum total calcium, ionized calcium, and hydroxyproline was highest for cows fed − 200 DCAD compared to those fed − 120 and 0 DCAD. Parathyroid hormone was highest for cows fed 0 DCAD compared to those fed − 120 and − 200 DCAD. Prepartum dry matter intake (DMI) was lower for − 200 and − 120 DCAD compared with 0 DCAD. Postpartum DMI was not different among treatments. Pre- and postpartum DCAD treatments did not affect total milk yield or milk fat, but percentage of milk protein increased in cows fed the negative prepartum DCAD treatments.
Effects of trade openness on environmental quality: Evidence from developing countries
Authors: Thuy Diem Pham Thi , Nguyen Hoai Trong
Published: March 2022

This paper uses the Bayesian model averaging approach to investigate the effects of trade openness on environmental quality for a sample of 64 developing countries during the period 2003–2017. Differing from Antweiler et al. (2001), Cole and Elliott (2003), and Managi et al. (2009), we bridge the gap by the inclusion of financial openness and renewable energy consumption as the conditioning factors that might be crucial for environmental quality. The empirical results reveal that trade openness in developing countries does not cause environmental degradation. Besides trade openness, our findings provide strong evidence in favor of financial openness and renewable energy consumption as two of the most important determinants of environmental quality by reducing carbon dioxide emissions. Meanwhile, inward FDI stock to domestic capital stock and income have harmful effects on environmental quality in the context of developing countries.