bluuebunny/crossref_metadata_2025 huggingface dataset:
Okanagan Waterways Past, Present and Future: Approaching Sustainability through Immersive Museum Exhibition
Authors: Dulic Aleksandra , Thorogood Miles , Sam Marlowe , Correia Maria , Alexis Sarah , Armstrong Jeanette
Published: November 2023

This paper presents Waterways Past, Present and Future, a research project and exhibition in Okanagan Syilx territory, aimed at increasing awareness of the relationship between people and water towards catalyzing sustainable water practices. The exhibition’s multi-channel audio-visual media was designed to immerse, provoke, destabilize, transform and move visitors to take responsibility for water. Drawing on many ways of knowing and doing in the creative process, the exhibition opens different entry points to the research, thus encouraging an interdisciplinary and cross-cultural audience to engage with it. Waterways’ contribution to sustainability discourse lies in its empowerment of collaborative inquiry as a way of knowing, understanding and representing our world. The epistemological dimensions of the exhibit present multiplicities embedded in the social life of water, inviting dialogues, shaping cultural narratives and developing new forms of creativity. Through the sensual process of immersion and activation of lateral thinking, the exhibition facilitates connections across cultures, connections that act as agents for social transformation. Waterways’ experiential journey transcends our personal and dominant socio-cultural patterns, reaching beyond normative structures to new creative realms shared ethical space.
Combination of Microalgae Method, Decantation, and Filtration for Domestic Wastewater Treatment
Authors: El-Moustaqim Khadija , El Bakraoui Houria , Mabrouki Jamal , Fouad Yasser , Slaoui Miloudia , Hmouni Driss , Benyeogor Mbadiwe S. , Igbigbi Tobore Louis
Published: November 2023

Phycoremediation of wastewater with microalgae is a viable option and is considered a process for cleaning up toxic waste using microalgae or macroalgae. Most water is modified by its use and must be treated before discharge. Given this situation, and following the example of other researchers around the world, our study focuses on the filtration method and combines it with the microalgae method to treat domestic wastewater. The aim of our work is to study the effects of using the microalgae system in combination with the decontamination and filtration system to reduce the nutrient content of domestic wastewater. The coupling of the two methods produced very significant results. However, the removal efficiencies for the filtered effluent increased to 86.34%, 100%, and 91.12% for COD, ammonia, and phosphate, respectively. The algae treatment offers an ecologically safe and less expensive system for nutrient removal and eliminates the need for tertiary treatment, which refers to the filtered treatment effluent, allowing us to conclude that the Chlorella vulgaris species has a very interesting influence on dissolved oxygen and that it had a very remarkable effect on COD, with a maximum reduction that reached 80%. The results obtained show that the phosphate content of the treated wastewater was significantly reduced during the cultivation period. In time, a decrease in solids was observed within the microalgae treatment system, influenced by the use of two different types of microalgae and the incorporation of the filtration system, which is based on the use of biosorption of methylene blue by biomass. The parameters analyzed in this study are hydrogen potential (pH), ammonia (NH3), phosphate ion (PO43−), chemical oxygen demand (COD), electrical conductivity (EC), total solids (TS), total dissolved solids (TDS), total suspended solids (TSS), nitrates, and dissolved oxygen (DO).
New Geoeducational Facilities in Central Mazovia (Poland) Disseminate Knowledge about Local Geoheritage
Authors: Górska-Zabielska Maria
Published: November 2023

Geoeducation is fundamental for safeguarding the abiotic world and its impact on the environment, which is inhabited by a society with ever-growing aspirations. However, current Earth and environmental science education in schools is insufficient. It requires creative and captivating methods that extend beyond traditional classroom settings, such as utilising new natural landscapes, in order to effectively implement geoeducation. New geological resources are unveiled during fieldwork or deep excavations. They can also be altered in situ through anthropogenic means to appear more visible to observers, particularly in remote tourism regions. As a geotourism product, these resources have the potential to serve as a catalyst for local economic growth. This article presents five geosites in central Mazovia, Poland, which were opened to the public in 2022 and 2023. Two Scandinavian erratic boulders, one of which has been developed, and three lapidaries with geotourism infrastructure are discussed. The research examines the significance of the erratic boulders for the natural and human environment.
Evaluating Future Streamflow Patterns under SSP245 Scenarios: Insights from CMIP6
Authors: Haleem Kashif , Khan Afed Ullah , Khan Jehanzeb , Ghanim Abdulnoor A. J. , Al-Areeq Ahmed M.
Published: November 2023

The potential impacts of climate change on water resources in the Upper Indus Basin of Pakistan, a region heavily reliant on these resources for irrigated agriculture. We employ state-of-the-art global climate models from the CMIP6 project under the SSP245 scenario to evaluate changes in river runoff using the Soil and Water Assessment Tool (SWAT). Our findings indicate that temperature fluctuations play a crucial role in streamflow dynamics, given that the primary sources of river runoff in the Upper Indus Basin are snow and glacier melting. We project a substantial increase of approximately 18% in both minimum and maximum temperatures, precipitation pattern increases of 13–17%, and a significant rise in streamflow by 19–30% in the future, driven by warmer temperatures. Importantly, our analysis reveals season-specific impacts of temperature, precipitation, and streamflow, with increasing variability in projected annual changes as we progress into the mid and late 21st century. To address these changes, our findings suggest the need for integrated strategies and action plans encompassing hydroelectricity generation, irrigation, flood prevention, and reservoir storage to ensure effective water resource management in the region.
E-Learning Platform Usage and Acceptance of Technology after the COVID-19 Pandemic: The Case of Transilvania University
Authors: Dimulescu Cristina
Published: November 2023

This research aims to examine the evolution of student attitudes toward the Transilvania University e-learning platform over a three-year period, encompassing the time before and after the COVID-19 pandemic. The study collected both quantitative and qualitative data through a structured online survey. Quantitative data were analyzed using descriptive statistics (frequency distribution) to measure changes in perceived ease of use, experiences, or degree of satisfaction, while qualitative responses were thematically analyzed to capture students’ comments about the platform within the Technology Acceptance Model (TAM) framework. The findings indicate an increased usage of the e-learning platform and satisfaction with the user interface post-pandemic, along with a reduction in reported technical issues. Moreover, a predominantly positive sentiment emerged from the thematic analysis of student feedback. These results provide universities with evidence that higher education facilitated by an e-learning platform is sustainable and capable of offering enriched learning experiences, extending beyond the pandemic context.
Disinfection of Rainwater for Economic Purposes
Authors: Zdeb Monika , Papciak Dorota
Published: November 2023

Rainwater storage systems are one of the elements of the implementation of sustainable water management. The use of rainwater in households or public buildings reduces the consumption of water supply water for purposes that do not require very good quality water. In crisis situations, rainwater could also be a source of water for drinking and hygiene. In order to use rainwater, it must comply with sanitary quality standards. This paper presents the results of research on the disinfection of rainwater and the possibility of its safe use in the economy as an alternative to tap water. The elements of the proposed pretreatment and disinfection system were selected adequately for the quality of the collected rainwater and its intended use. The aim was to obtain water safe for drinking and hygienic purposes. Rainwater was collected from a roof covered with ceramic tiles, and then subjected to prefiltration, ultrafiltration and disinfection with UV rays. Water before and after treatment was characterized on the basis of a number of microbiological parameters (total number of bacteria at 37 °C and 22 °C; number of coliform bacteria, Escherichia coli, Enterococci, Pseudomonas aeruginosa) and the content of nutrients (TOC, ammonium nitrogen, nitrates, nitrites, phosphates). The use of ultraviolet radiation allowed for the complete removal of indicator bacteria and a significant reduction in the total number of bacteria, from nearly 2500 CFU/mL to 25 CFU/mL for bacteria at 22 °C and from 2010 CFU/mL to 18 CFU/mL for bacteria at 37 °C. The effectiveness of rainwater disinfection, its microbiological stability after disinfection and the time after which the bacterial microflora regenerates, as well as the possibility of using rainwater for drinking and hygienic purposes after disinfection, was determined.
Enhancing Supply Chain Efficiency: A Two-Stage Model for Evaluating Multiple Sourcing and Extra Procurement Strategy Optimization
Authors: Kim Chong-Keun , Lee Chanho , Kim DongKyun , Cha Hyungjoo , Cheong Taesu
Published: November 2023

This paper presents a two-stage mathematical model aimed at enhancing supply chain efficiency by evaluating multiple sourcing strategies and optimizing extra procurement. In the first stage, the model minimizes the gap between Sales and Operation Planning (SOP) and maximum product quantity achievable with current inventory levels, setting the foundation for understanding resource requirements. The second stage focuses on extra procurement strategy optimization, considering lead times, cost-effectiveness. We analyze the trade-offs between multiple sourcing and single vendor approaches, providing valuable insights for supply chain decision-makers. This research offers a practical framework to improve supply chain efficiency, reduce gaps, and enhance customer satisfaction while strengthening supply chain resilience in the face of unforeseen challenges, as demonstrated by the lessons from the COVID-19 pandemics.
Determinants of Herders’ Satisfaction with the Grassland Ecosystem Compensation Policy: A Case Study of Gansu Province, China
Authors: Du Sanqiang , Cheng Yunxiang , An Dong
Published: November 2023

This study investigated herders’ satisfaction with the implementation effects of the Grassland Ecosystem Compensation Policy (GECP) in Sunan (subsidy hierarchization) and Gannan (subsidy harmonization), China. Survey data from 140 randomly selected herder households were analyzed using descriptive statistics and ordered logistic regression to identify the factors influencing herders’ satisfaction. The results showed that in Sunan, 47.89% of respondents expressed satisfaction with the GECP. Their satisfaction positively correlated with changes in native grass, ecological compensation income, and reduced inedible grass. Conversely, it exhibited negative associations with the ethnic background of the household head, livestock numbers, and willingness to relocate. In Gannan, a substantial level of dissatisfaction prevailed (69.57%). However, satisfied herders had connections with changes in native grass, income diversity, and ecological compensation income. Significantly, this study highlights that ecological compensation income and changes in native grass consistently influence herders’ satisfaction regardless of the subsidy design. These findings offer valuable insights for improving herders’ satisfaction with the implementation effects of the GECP in regions with diverse ecological subsidy designs. Additionally, it presents a fresh perspective for scholars to analyze the GECP under different ecological subsidy frameworks further.
Advancing Shear Capacity Estimation in Rectangular RC Beams: A Cutting-Edge Artificial Intelligence Approach for Assessing the Contribution of FRP
Authors: Ezami Nima , Özyüksel Çiftçioğlu Aybike , Mirrashid Masoomeh , Naderpour Hosein
Published: November 2023

Shear strength prediction in FRP-bonded reinforced concrete beams is crucial for ensuring structural integrity and safety. In this extensive investigation, advanced machine learning algorithms are harnessed to achieve precise shear strength predictions for rectangular RC beams reinforced with FRP sheets. The aim of this research is to enhance the accuracy and reliability of shear strength estimation, providing valuable insights for the design and assessment of FRP-strengthened structures. The primary contributions of this study lie in the meticulous comparison of various machine learning algorithms, including Xgboost, Gradient Boosting, Random Forest, AdaBoost, K-nearest neighbors, and ElasticNet. Through comprehensive evaluation based on predictive performance, the most suitable model for accurately estimating the shear strength of FRP-reinforced rectangular RC beams is identified. Notably, Xgboost emerges as the superior performer, boasting an impressive R2 value of 0.901. It outperforms other algorithms and demonstrates the lowest RMSE, MAE, and MAPE values, establishing itself as the most accurate and reliable predictor. Furthermore, a sensitivity analysis is conducted using artificial neural networks to assess the influence of input variables. This additional research facet sheds light on the critical factors shaping shear strength outcomes. The study, as a whole, represents a substantial contribution to advancing the development of accurate and dependable prediction models. The practical implications of this work are far-reaching, particularly for engineering applications in the realm of structures reinforced with FRP. The findings have the potential to transform the approach to the design and assessment of such structures, elevating safety, efficiency, and performance to new heights.
Enhancing Water-Sensitive Urban Design in Chiang Mai through a Research–Design Collaboration
Authors: Wanitchayapaisit Chulalux , Charoenlertthanakit Nadchawan , Surinseng Vipavee , Yaipimol Ekachai , Rinchumphu Damrongsak , Suppakittpaisarn Pongsakorn
Published: November 2023

Water-sensitive urban design (WSUD) is a subset of nature-based solutions (NbSs) that are implemented worldwide. However, the WSUD guidelines in some local contexts, such as Southeast Asia, remain unclear both for ecological and cultural reasons. This study aims to gather collaborations between researchers, designers, and laypeople in WSUD, which have the potential to be implemented to address water quality issues. The study consisted of three stages: site selection, a design workshop, and public interviews. Utilizing geo-design principles and geographical data, the potential pilot site was identified: a vacant space next to the Tha Phae Gate Plaza. A two-day workshop with landscape design experts yielded six conceptual designs, focusing on diverse themes such as water treatment, plant-based solutions, educational opportunities, and cultural enrichment. Public interviews provided insights into the community’s perspectives on stormwater management, desired amenities, environmental considerations, and governance concerns. The results highlighted a collective interest in using NbSs for stormwater treatment and enhancing the area’s recreational and educational potential. This study offers a comprehensive approach to addressing water quality issues in urban settings while considering local cultural, recreational, and environmental needs.
Exploration on the Innovation Model of County Urbanization Development with the Resource Constraints in China
Authors: Zhou Runchen , Guo Yang , Wu Yuzhe
Published: November 2023

The 14th Five-Year Plan for China proposes to promote the urbanization process from a county perspective, presenting an unprecedented opportunity to develop county urbanization. However, in the context of relatively limited resources, insufficient funds, and comparatively lower land values in Chinese counties, how to promote county urbanization remains the primary challenge, which has not been studied yet. This study first analyzes the necessity of promoting county urbanization from four dimensions: national development strategy, industrial policy development, local government status, and enterprise development demands using literature research and survey interviews. Based on field research conducted in 32 counties in China, the strengths, weaknesses, opportunities, and threats (SWOT) analysis is carried out on the county urbanization process in this study. By analyzing the developmental status and challenges of major urban areas, expansion areas of counties, and their surrounding countryside, this paper proposes a “three-level gradient integration” concept for the spatial integration of county urbanization. Furthermore, the mechanism of multi-agent linkage to promote county urbanization was explored based on the theory of urban governance and the characteristics of county urbanization. Subsequently, relying on the analysis of policies, such as land acquisition and integrated land improvement, and adopting modes, such as “Investor + Engineering Procurement Construction + Operation” (investor + EPC + O) and renovate–operate–transfer (ROT), we propose a pathway for promoting county urbanization through the linkage of government, enterprises, and residents. This study provides insight into promoting the county urbanization process. Additionally, each country faces the common issue of how to use limited resources to promote regional development, and this article can provide valuable insights and inspiration for addressing this shared issue by the principle of adapting to local conditions and adhering to the concept of efficient market and proactive government.
Segmenting Fitness Center Customers: Leveraging Perceived Ethicality for Enhanced Loyalty, Trust, and Word-of-Mouth Communication
Authors: Paschalidou Katerina , Tsitskari Efi , Alexandris Kostas , Karagiorgos Thomas , Filippou Dionisios
Published: November 2023

In an era marked by a growing emphasis on business ethics and sustainability, fitness centers face a compelling need to align their practices with their members’ perceived ethical values. To explore the role of ethics in the fitness industry’s expanding business landscape, this study draws upon established theories in consumer-perceived ethicality (CPE), business ethics, and customer segmentation strategies. The paper’s objectives were to adapt and validate the CPE scale for the Greek context and categorize fitness center members based on their perceived ethicality and to examine the impact of perceived ethicality on loyalty, word-of-mouth communication, and trust towards fitness centers. The research involved 286 fitness center members who completed a questionnaire measuring CPE, loyalty, trust, and word-of-mouth communication. Utilizing clustering analysis, two distinct consumer segments emerged, each demonstrating unique patterns of perceived ethicality. Intriguingly, both the high- and low-CPE groups assigned considerable importance to word-of-mouth communication, followed by trust and loyalty. These findings provide valuable insights for businesses in the fitness industry seeking to enhance their ethical reputation and bolster customer retention. Furthermore, the translated CPE scale holds the potential to contribute significantly to the ongoing discourse on consumer behavior regarding business ethics and sustainability within the fitness sector.
Supporting Sustainable Futures in Retail: An Exploratory Study on Worker Health, Safety and Wellbeing in Australia
Authors: Karanikas Nektarios , Martinez-Buelvas Laura , Sav Adem
Published: November 2023

Health, safety, and wellbeing (HSW) have been studied widely in different contexts and are associated with social, organisational, and human sustainability. However, there is limited research about HSW in retail that includes both workers and managers to offer a more holistic and inclusive perspective. To fill this gap, this exploratory and descriptive cross-sectional study employed a 21-item quantitative and 3-item qualitative online survey and engaged a representative sample of 271 workers and 109 managers/owners in Australia. The results showed retailers provide a reasonably adequate technical and social work environment, and workers perceive their job tasks as averagely challenging. Nevertheless, workers still experienced adverse HSW effects frequently, partially attributed to a poor organisational environment. Workers and managers were markedly aligned regarding the current HSW status in Australian retail and agreed that health and safety were not focal areas of their businesses. Worryingly, both groups viewed the unfavourable impacts of work as inescapable, indicating a commonly shared fatalistic perspective that does not align with sustainability practices. Although workers appreciated their employment-related benefits, they also acknowledged that their HSW was not fully supported, presenting unique opportunities for the workplace and policymakers to address such issues and support the economic and social sustainability of retailers.
Selective Chemical Filters for VOF3: Tailoring MgF2 Filter Selectivity through Surface Chemistry
Authors: Jouffret Laurent , Hiltbrunner Jean-Michel , Petit Elodie , Selmi Ania , Morel Bertrand , Dubois Marc
Published: November 2023

In order to synthesize chemical filters for the selective removal of volatile fluorides, commercial magnesium fluoride MgF2 with high specific surface area (HSA) was investigated. The amount of -OH groups substituting fluorine is not negligible, partly due to the high surface area, but also due to the synthesis route. These hydroxyl groups induce a Lewis basicity on the surface of metal fluorides. The amount of these Lewis basic sites has been tailored using fluorination with F2 gas. The sorption of VOF3, used as model gas, onto these fluorides was investigated. The versatility of surface chemistry as a function of a number of Lewis basic sites opens the way to filter selectivity mixture of volatile fluorides depending on their Lewis acidity. HSA MgF2 acts as a stable matrix towards the gas to be purified, and the selectivity may be achieved by a higher Lewis acidity of the gaseous impurity.
A Note on Shape Vector Fields on Hypersurfaces
Authors: Al-Shaikh Suha B.
Published: November 2023

In this paper, we initiate the study of shape vector fields on the hypersurfaces of a Riemannian manifold. We use a shape vector field on a compact hypersurface of a Euclidean space to obtain a characterization of round spheres. We also find a condition, under which a shape vector field that is on a compact hypersurface of a Euclidean space is a Killing vector field.
The Separability Problem in Two Qubits Revisited
Authors: Gnonfin Honorine , Gouba Laure
Published: November 2023

In this paper, we present an overview of the progress in the separability problem in bipartite systems, more specifically in a two quantum bits (qubits) system, from the separability criterion based on Bell’s inequalities in 1964 to the recent separability criteria.
Critical Thinking Skills Enhancement through System Dynamics-Based Games: Insights from the Project Management Board Game Project
Authors: Barnabè Federico , Armenia Stefano , Nazir Sarfraz , Pompei Alessandro
Published: November 2023

This study aims to explore and discuss the role of systems thinking and system dynamics-assisted games in enhancing critical thinking skills in learners. In more detail, the study relies on the use of a system dynamics-based interactive learning environment related to project management issues, followed by systems thinking-supported debriefing sessions. The interactive learning environment was developed and used in the form of a single-player, online, computer-based game. The game was designed to mimic all the necessary planning and operational activities needed to organize a wedding ceremony. The acquisition of critical thinking skills in learners was evaluated in three main ways: (1) players’ performances were analyzed through a scoring system embedded in the game that considers several performance dimensions; (2) feedback from the players was collected and analyzed by using basic content analysis; (3) players’ performances were analyzed using five main categories of structures that are typical of project management domains, i.e., project features, the rework cycle, project control, ripple effects, and knock-on effects. The findings show that the joint use of system dynamics and systems thinking tools and principles within a gaming environment has the potential to facilitate and enhance the acquisition of critical thinking skills in learners and may also provide valid support for educators and practitioners interested in the enhancement of project management skills.
Recent Strategies for the Remediation of Textile Dyes from Wastewater: A Systematic Review
Authors: Tripathi Manikant , Singh Sakshi , Pathak Sukriti , Kasaudhan Jahnvi , Mishra Aditi , Bala Saroj , Garg Diksha , Singh Ranjan , Singh Pankaj , Singh Pradeep Kumar , Shukla Awadhesh Kumar , Pathak Neelam
Published: November 2023

The presence of dye in wastewater causes substantial threats to the environment, and has negative impacts not only on human health but also on the health of other organisms that are part of the ecosystem. Because of the increase in textile manufacturing, the inhabitants of the area, along with other species, are subjected to the potentially hazardous consequences of wastewater discharge from textile and industrial manufacturing. Different types of dyes emanating from textile wastewater have adverse effects on the aquatic environment. Various methods including physical, chemical, and biological strategies are applied in order to reduce the amount of dye pollution in the environment. The development of economical, ecologically acceptable, and efficient strategies for treating dye-containing wastewater is necessary. It has been shown that microbial communities have significant potential for the remediation of hazardous dyes in an environmentally friendly manner. In order to improve the efficacy of dye remediation, numerous cutting-edge strategies, including those based on nanotechnology, microbial biosorbents, bioreactor technology, microbial fuel cells, and genetic engineering, have been utilized. This article addresses the latest developments in physical, chemical, eco-friendly biological and advanced strategies for the efficient mitigation of dye pollution in the environment, along with the related challenges.
The Effects of Different Soil Component Couplings on the Methylation and Bioavailability of Mercury in Soil
Authors: Qin Aming , Ran Shu , He Tianrong , Yin Deliang , Xu Yiyuan
Published: November 2023

Soil composition can influence the chemical forms and bioavailability of soil mercury (Hg). However, previous studies have predominantly focused on the influence of individual components on the biogeochemical behavior of soil Hg, while the influence of various component interactions among several individual factors remain unclear. In this study, artificial soil was prepared by precisely regulating its components, and a controlled potted experiment was conducted to investigate the influence of various organic and inorganic constituents, as well as different soil textures resulting from their coupling, on soil Hg methylation and its bioavailability. Our findings show that inorganic components in the soils primarily exhibit adsorption and fixation effects on Hg, thereby reducing the accumulation of total mercury (THg) and methylmercury (MeHg) in plants. It is noteworthy that iron sulfide simultaneously resulted in an increase in soil MeHg concentration (277%). Concentrations of THg and MeHg in soil with peat were lower in rice but greater in spinach. A correlation analysis indicated that the size of soil particles was a crucial factor affecting the accumulation of Hg in plants. Consequently, even though fulvic acid activated soil Hg, it significantly increased the proportion of soil particles smaller than 100.8 μm, thus inhibiting the accumulation of Hg in plants, particularly reducing the concentration of THg (93%) and MeHg (85%) in water spinach. These results demonstrate that the interaction of organic and inorganic components can influence the biogeochemical behavior of soil Hg not only through their chemical properties, but also by altering the soil texture.
Association between Dental Scores and Saliva Uremic Toxins
Authors: Rigothier Claire , Catros Sylvain , Bénard Antoine , Samot Johan , Quintin Olivier , Combe Christian , Larabi Islam , Massy Ziad , Alvarez Jean-Claude
Published: November 2023

Dental health is frequently altered in patients with chronic kidney disease. We conducted a prospective study on dental health in CKD patients with a specific interest in the association between dental health issues and the accumulation of uremic toxins in the saliva. A total of 88 patients were included in the study, with chronic kidney disease stage 2 to 5 (without kidney replacement). We analysed the total concentrations of eight uremic toxins (trimethylamine N-oxide -TMAO-, Indoxyl Sulfate, P-cresyl-sulfate, Indole 3-acetic acid, 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid -CMPF-, Kynurenine, Hippuric acid and Phenylacetylglutamine) and three precursors of uremic toxins (Tyrosine, Phenylalanine and Tryptophan) in the saliva using LC-MS/MS. We observed, for the first time, the association between various dental scores: DMFT, FST, CPITN, and OHIS, and saliva uremic toxins and precursors: TMAO, indoxyl sulfate, or hippuric acid. Further prospective interventional studies are required to confirm our results.
Laboratory Surveillance of Acinetobacter spp. Bloodstream Infections in a Tertiary University Hospital during a 9-Year Period
Authors: Spiliopoulou Anastasia , Giannopoulou Ioanna , Assimakopoulos Stelios F. , Jelastopulu Eleni , Bartzavali Christina , Marangos Markos , Paliogianni Fotini , Kolonitsiou Fevronia
Published: November 2023

Multidrug-resistant Acinetobacter baumannii infections have become a threat for public health worldwide. The aim of the present study was to follow-up resistance patterns of Acinetobacter spp. bloodstream isolates in a Tertiary University Hospital over the last nine years, from 2014 to 2022. Susceptibility patterns were followed for the following antimicrobial agents: amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, imipenem, meropenem, tigecycline, trimethoprim/sulfamethoxazole, and colistin. Minimal inhibitory concentration (MIC) values to ampicillin/sulbactam, cefepime, ceftazidime, minocycline, piperacillin/tazobactam were evaluated from 2020 to 2023. During the study period, 853 Acinetobacter spp. bloodstream infections (BSIs) were recorded, accounting for 5.36% of all BSIs. A. baumannii was isolated in 795 cases (93.2%), during the study period. Most BSIs were recorded in adult intensive care units (ICU) (46.2%) and medical wards (42%). Among A. baumannii isolates, 4.5% were multidrug-resistant, 84.7% were extensively drug-resistant, and 8.5% were pandrug-resistant. Resistance to carbapenems was over 95%. Resistance to tigecycline increased significantly during the last years of the study (2020–2022); A. baumannii isolates with MIC ≤ 2 μg/mL accounted for 28.5% of all isolates. Resistance to colistin exhibited an increasing pattern up to 42.2% in 2022. Increasing resistance rates and the evolution of pandrug-resistant isolates call for the urgent application of preventive and response actions.
Challenges for Precise Subtyping and Sequencing of a H5N1 Clade 2.3.4.4b Highly Pathogenic Avian Influenza Virus Isolated in Japan in the 2022–2023 Season Using Classical Serological and Molecular Methods
Authors: Komu James G. , Nguyen Hiep Dinh , Takeda Yohei , Fukumoto Shinya , Imai Kunitoshi , Takemae Hitoshi , Mizutani Tetsuya , Ogawa Haruko
Published: November 2023

The continuous evolution of H5Nx highly pathogenic avian influenza viruses (HPAIVs) is a major concern for accurate diagnosis. We encountered some challenges in subtyping and sequencing a recently isolated H5N1 HPAIV strain using classical diagnostic methods. Oropharyngeal, conjunctival, and cloacal swabs collected from a dead white-tailed eagle (Haliaeetus albicilla albicilla) were screened via real-time RT-PCR targeting the influenza A virus matrix (M) gene, followed by virus isolation. The hemagglutination inhibition test was applied in order to subtype and antigenically characterize the isolate using anti-A/duck/Hong Kong/820/80 (H5N3) reference serum or anti-H5N1 cross-clade monoclonal antibodies (mAbs). Sequencing using previously reported universal primers was attempted in order to analyze the full-length hemagglutinin (HA) gene. Oropharyngeal and conjunctival samples were positive for the M gene, and high hemagglutination titers were detected in inoculated eggs. However, its hemagglutination activity was not inhibited by the reference serum or mAbs. The antiserum to a recently isolated H5N1 clade 2.3.4.4b strain inhibited our isolate but not older strains. A homologous sequence in the previously reported forward primer and HA2 region in our isolate led to partial HA gene amplification. Finally, next-generation sequencing confirmed the isolate as H5N1 clade 2.3.4.4b HPAIV, with genetic similarity to H5N1 strains circulating in Japan since November 2021.
African Swine Fever: Transmission, Spread, and Control through Biosecurity and Disinfection, Including Polish Trends
Authors: Juszkiewicz Małgorzata , Walczak Marek , Woźniakowski Grzegorz , Podgórska Katarzyna
Published: November 2023

African swine fever is a contagious disease, affecting pigs and wild boars, which poses a major threat to the pig industry worldwide and, therefore, to the agricultural economies of many countries. Despite intensive studies, an effective vaccine against the disease has not yet been developed. Since 2007, ASFV has been circulating in Eastern and Central Europe, covering an increasingly large area. As of 2018, the disease is additionally spreading at an unprecedented scale in Southeast Asia, nearly ruining China’s pig-producing sector and generating economic losses of approximately USD 111.2 billion in 2019. ASFV’s high resistance to environmental conditions, together with the lack of an approved vaccine, plays a key role in the spread of the disease. Therefore, the biosecurity and disinfection of pig farms are the only effective tools through which to prevent ASFV from entering the farms. The selection of a disinfectant, with research-proven efficacy and proper use, taking into account environmental conditions, exposure time, pH range, and temperature, plays a crucial role in the disinfection process. Despite the significant importance of ASF epizootics, little information is available on the effectiveness of different disinfectants against ASFV. In this review, we have compiled the current knowledge on the transmission, spread, and control of ASF using the principles of biosecurity, with particular attention to disinfection, including a perspective based on Polish experience with ASF control.
The Association between Levels of Trust in the Healthcare System and Influenza Vaccine Hesitancy among College Students in Israel
Authors: Dopelt Keren , Abudin Anuar , Yukther Sophie , Shmukler Tatyana , Davidovitch Nadav
Published: November 2023

Influenza is a contagious respiratory disease caused by the influenza virus. Vaccination proves an effective approach to preventing influenza and minimizing the risk of experiencing associated complications. However, the influenza vaccine coverage rate among Israeli college students is low due to a sense of complacency, lack of knowledge, and vaccine hesitancy. The current study examined the relationship between the level of trust in the healthcare system and influenza vaccine hesitancy among college students in Israel. This cross-sectional study was conducted via an online questionnaire in April–May 2023. In total, 610 students were surveyed, of whom 57% had been vaccinated against influenza in the past; however, only 12% were vaccinated this year. Negative, significant, and moderate relationships were found between the level of trust in the healthcare system and influenza vaccine hesitancy. Students who had been vaccinated in the past had a higher level of trust in the healthcare system and a lower level of vaccination hesitancy. The linear regression model revealed that the variables of being a woman, not Jewish, vaccinated, and trusting the Ministry of Health, family doctor, and health professionals were associated with a decrease in vaccine hesitancy. These findings are in line with previous research in the field. Based on the present results, it may be advisable to develop intervention programs aimed at increasing confidence in the healthcare system and vaccinations by providing knowledge and addressing students’ concerns regarding vaccination.
The Promotion of Humoral Immune Responses in Humans via SOCS1-Mediated Th2-Bias Following SARS-CoV-2 Vaccination
Authors: Liu Xiaoyu , Han Junyong , Cui Renjie , Peng Meifang , Song Huaidong , Li Rui , Chen Gang
Published: November 2023

The effectiveness of SARS-CoV-2 vaccines varies among individuals. During the COVID-19 global pandemic, SARS-CoV-2 infection showed significant Th1 characteristics, suggesting that the immune disorder and production of SARS-CoV-2 antibodies may be related to Th1/Th2 bias. However, the molecular mechanisms underlying Th1/Th2 bias effects on host immune responses to viruses remain unclear. In this study, the top three subjects with the highest and lowest changes in anti-SARS-CoV-2 antibodies after receiving three doses of SARS-CoV-2 vaccination were selected and defined as the elevated group (E) and the control group (C), respectively. Peripheral blood was collected, single-cell sequencing was performed before and after the third dose of the SARS-CoV-2 vaccine, and the changes in T cell clusters were analyzed. Compared with the C group, the Treg pre-vaccination proportion was lower in E, while the post-vaccination proportion was higher, suggesting that Tregs may be crucial in this process. Differential analysis results of Tregs between the two groups revealed that differentially expressed genes (DEGs) were significantly enriched in the IL4 pathway. Correlation analysis between DEGs and serum antibody showed that the expression of NR4A2, SOCS1, and SOCS3 in Tregs was significantly correlated with serum antibodies, suggesting that the immune response in E group changed to Th2 bias, thereby promoting host humoral immune responses. On the other hand, antibody-related genes SOCS1 and NR4A2, as well as lnc-RNA MALAT1 and NEAT1, were highly expressed in the CD4-MALAT1 subclusters. In summary, our study revealed that Th2 bias promotes humoral immune responses in humans by increasing SOCS1 in T cells after SARS-CoV-2 vaccination. Moreover, NR4A2, SOCS1, MALAT1, and NEAT1 were identified as the potential key biomarkers or treatment targets for enhanced SARS-CoV-2 antibody production by influencing the Th1/Th2 balance in T cells. Our findings have important implications for population stratification and tailored therapeutics for more effective SARS-CoV-2 vaccines.
Longitudinal Analysis of Neuraminidase and Hemagglutinin Antibodies to Influenza A Viruses after Immunization with Seasonal Inactivated Influenza Vaccines
Authors: Sergeeva Mariia V. , Romanovskaya-Romanko Ekaterina A. , Krivitskaya Vera Z. , Kudar Polina A. , Petkova Nadezhda N. , Kudria Kira S. , Lioznov Dmitry A. , Stukova Marina A. , Desheva Yulia A.
Published: November 2023

Neuraminidase (NA)-based immunity could reduce the harmful impact of novel antigenic variants of influenza viruses. The detection of neuraminidase-inhibiting (NI) antibodies in parallel with anti-hemagglutinin (HA) antibodies may enhance research on the immunogenicity and duration of antibody responses to influenza vaccines. To assess anti-NA antibodies after vaccination with seasonal inactivated influenza vaccines, we used the enzyme-linked lectin assay, and anti-HA antibodies were detected in the hemagglutination inhibition assay. The dynamics of the anti-NA antibody response differed depending on the virus subtype: antibodies to A/H3N2 virus neuraminidase increased later than antibodies to A/H1N1pdm09 subtype neuraminidase and persisted longer. In contrast to HA antibodies, the fold increase in antibody titers to NA after vaccination poorly depended on the preexisting level. At the same time, NA antibody levels after vaccination directly correlated with titers before vaccination. A difference was found in response to NA antigen between split and subunit-adjuvanted vaccines and in NA functional activity in the vaccine formulations.
Effective Adsorption of Chlorinated Polyfluoroalkyl Ether Sulfonates from Wastewater by Nano-Activated Carbon: Performance and Mechanisms
Authors: Yi Hao , Chen Xiaolin , Liu Zewei , Xi Hongxia , Ding Zecong , Cui Kai , Hu Yongyou
Published: November 2023

Chlorinated polyfluoroalkyl ether sulfonates (F-53B) were often used as mist suppressants in the chrome plating industry, resulting in the large discharge of F-53B-containing electroplating wastewater into the aquatic environment. Due to the high toxicity of F-53B, increasing attention has been paid to its efficient removal from wastewater. In this study, three nano-activated carbons were successfully prepared from coconut shell carbons by a simple one-step KOH activation method. The nitrogen adsorption/desorption experiments showed that the synthesized coconut shell activated carbons possessed a well-developed nano-pore structure, which was favorable for the adsorption of F-53B. The results suggested that the adsorption of F-53B on the coconut shell activated carbons followed pseudo-second-order kinetics and was better fitted in the Langmuir isotherm, indicating that the adsorption of F-53B was mainly controlled by chemical adsorption and was mainly monolayer adsorption. Theoretical calculation results revealed that the faster adsorption rate of F-53B on CSAC_800 than on CSAC_600 and CSAC_700 could be contributed to the lower adsorption energy of F-53B on CSAC_800 and the higher self-diffusion coefficients of F-53B in CSAC_800. The higher adsorption capacity of CSAC_800 (qm = 537.6 mg·g−1) for F-53B than that of CSAC_600 (qm = 396.83 mg·g−1) and CSAC_700 (qm = 476.19 mg·g−1) could be attributed to the higher specific surface area and larger number of adsorption sites of CSAC_800. The results of this study demonstrate that coconut shell activated carbons with a well-developed nano-pore structure are an effective adsorbent for F-53B removal and have a good application prospect.
Effect of Aeration on the Cavitation Characteristics of the Control Valve in Hydro-Driven Ship Lifts
Authors: Wang Jiao , Hu Yaan , Chen Liang , Hu Ruichang , Yuan Hao
Published: November 2023

In hydro-driven ship lifts, plunger valves and fixed cone valves are the most suitable structures for achieving accurate flow control under a wide range of flow conditions. In order to inhibit cavitation in these valve structures, experiments were conducted in which forced aeration was applied before the valve. The cavitation phenomena and aerated flow regime were observed through a transparent glass pipe, and the cavitation noise characteristics were recorded using a hydrophone. The test results show that aeration can reduce the valve working cavitation number, albeit to a limited degree (<5%). Based on the sound velocity theory of aerated flow and the experimental results, the relationship between the aeration concentration and the cavitation inhibition efficiency was obtained. When the aeration concentration is approximately 0.1%, cavitation can be significantly inhibited via forced aeration before the valve. Once the aeration concentration reaches 0.9%, the cavitation inhibition efficiency becomes saturated. The research results presented in this paper provide a reference for the cavitation inhibition of industrial valves.
Evaluation of Future-Integrated Urban Water Management Using a Risk and Decision Analysis Framework: A Case Study in Denver–Colorado Metro Area (DCMA)
Authors: He Bowen , Zheng Han , Guan Qun
Published: November 2023

This study examines the DCMA concerning the future risk of the water security status. We considered three risk factors: population growth, economic growth, and natural water supply–demand differences. In the risk analysis part, we consulted with experts from several sectors including academia, Non-Governmental Organizations (NGOs), and industry, to predict that the probability of future water stresses in high-, medium-, and low-risk scenarios are 0.73, 0.24, and 0.03, respectively. In the decision analysis part, we adopted two multiple criteria decision analysis (MCDA) approaches that include multiple attribute value theory (MAVT) and analytic hierarchy process (AHP) methods to evaluate the best alternative decision to alleviate future water stresses in the DCMA. The sensitivity analysis demonstrates that, although expanding existing water reservation might be a solution to tackle the challenge, the best option really closely connects to the weighting scheme of the criteria considered in the framework. This study provides a valuable risk and decision analysis framework to analyze the water security status associated with the future water supply and demand gap decrease caused by three risk factors: population growth, climate change, and natural water supply.
Optimization Research on the Space-V-Type Biomimetic Surface Grooves of a Marine Centrifugal Pump
Authors: Li Hua , Yu Zifeng , Hua Runan , Li Chenqi , Guo Chao , Liu Houlin , Dong Liang
Published: November 2023

The biomimetic surface with Space-V grooves can effectively reduce flow resistance and noise. Our investigation was in order to further enhance the drag reduction and noise reduction performance of a marine centrifugal pump with Space-V-groove-shaped biomimetic surfaces. A regression equation was established with response surface methodology between the total sound pressure level and the height (h), width (s), and spacing (b) of the biomimetic groove structure. The interaction effects of various parameters on the total sound pressure level were analyzed, and the parameter range was determined at the lowest total sound pressure level. The hydraulic performance and interior noise of the model before and after optimization were compared. The results showed that the total sound pressure level initially decreased and then increased with increasing groove height. Similarly, with an increase in groove width, the total sound pressure level decreased at first, then increased. When the height of the bionic groove is 0.5–0.7 mm, the groove width is 0.4–0.7 mm, the groove spacing is 0.7–1.3 mm, and the total sound pressure level of the centrifugal pump is the smallest, which is 180–182 dB. On the other hand, the total sound pressure level increased as groove spacing increased. Through the use of an optimized Space-V groove model, under rated working conditions, the model head is increased by 0.27 m and the efficiency is increased by 1.21%. In addition, the optimized model has excellent drag and noise reduction performance, with the drag reduction rate of 3.73% and noise reduction rate of 1.81%, which are, respectively, increased by 0.87% and 0.45% compared with before optimization. The performance of centrifugal pumps for ships can be greatly improved.