bluuebunny/crossref_metadata_2025 huggingface dataset:
ASA Status, NPPA/NPPB Haplotype and Coronary Artery Disease Have an Impact on BNP/NT-proBNP Plasma Levels
Authors: Hahn Markus , Stamer Ulrike M. , Luedi Markus M. , Book Malte , Rieder Heinz U. , Stüber Frank
Published: February 2022

Plasma concentrations of natriuretic peptides (NP) contribute to risk stratification and management of patients undergoing non-cardiac surgery. However, genetically determined variability in the levels of these biomarkers has been described previously. In the perioperative setting, genetic contribution to NP plasma level variability has not yet been determined. A cohort of 427 patients presenting for non-cardiac surgery was genotyped for single-nucleotide polymorphisms (SNPs) from the NPPA/NPPB locus. Haplotype population frequencies were estimated and adjusted haplotype trait associations for brain natriuretic peptide (BNP) and amino-terminal pro natriuretic peptide (NT-proBNP) were calculated. Five SNPs were included in the analysis. Compared to the reference haplotype TATAT (rs198358, rs5068, rs632793, rs198389, rs6676300), haplotype CACGC, with an estimated frequency of 4%, showed elevated BNP and NT-proBNP plasma concentrations by 44% and 94%, respectively. Haplotype CGCGC, with an estimated frequency of 9%, lowered NT-proBNP concentrations by 28%. ASA classification status III and IV, as well as coronary artery disease, were the strongest predictors of increased NP plasma levels. Inclusion of genetic information might improve perioperative risk stratification of patients based on adjusted thresholds of NP plasma levels.
MicroRNA-139, an Emerging Gate-Keeper in Various Types of Cancer
Authors: Stavast Christiaan J. , van Zuijen Iris , Erkeland Stefan J.
Published: February 2022

Mounting data show that MIR139 is commonly silenced in solid cancer and hematological malignancies. MIR139 acts as a critical tumor suppressor by tuning the cellular response to different types of stress, including DNA damage, and by repressing oncogenic signaling pathways. Recently, novel insights into the mechanism of MIR139 silencing in tumor cells have been described. These include epigenetic silencing, inhibition of POL-II transcriptional activity on gene regulatory elements, enhanced expression of competing RNAs and post-transcriptional regulation by the microprocessor complex. Some of these MIR139-silencing mechanisms have been demonstrated in different types of cancer, suggesting that these are more general oncogenic events. Reactivation of MIR139 expression in tumor cells causes inhibition of tumor cell expansion and induction of cell death by the repression of oncogenic mRNA targets. In this review, we discuss the different aspects of MIR139 as a tumor suppressor gene and give an overview on different transcriptional mechanisms regulating MIR139 in oncogenic stress and across different types of cancer. The novel insights into the expression regulation and the tumor-suppressing activities of MIR139 may pave the way to new treatment options for cancer.
Role of Mitochondrial Stress Response in Cancer Progression
Authors: Lee Yu Geon , Park Do Hong , Chae Young Chan
Published: February 2022

Mitochondria are subcellular organelles that are a hub for key biological processes, such as bioenergetic, biosynthetic, and signaling functions. Mitochondria are implicated in all oncogenic processes, from malignant transformation to metastasis and resistance to chemotherapeutics. The harsh tumor environment constantly exposes cancer cells to cytotoxic stressors, such as nutrient starvation, low oxygen, and oxidative stress. Excessive or prolonged exposure to these stressors can cause irreversible mitochondrial damage, leading to cell death. To survive hostile microenvironments that perturb mitochondrial function, cancer cells activate a stress response to maintain mitochondrial protein and genome integrity. This adaptive mechanism, which is closely linked to mitochondrial function, enables rapid adjustment and survival in harsh environmental conditions encountered during tumor dissemination, thereby promoting cancer progression. In this review, we describe how the mitochondria stress response contributes to the acquisition of typical malignant traits and highlight the potential of targeting the mitochondrial stress response as an anti-cancer therapeutic strategy.
E-Cigarette Aerosols Promote Oral S. aureus Colonization by Delaying an Immune Response and Bacterial Clearing
Authors: Cátala-Valentín Alma R. , Almeda Jasmine , Bernard Joshua N. , Cole Alexander M. , Cole Amy L. , Moore Sean D. , Andl Claudia D.
Published: February 2022

E-cigarette (e-cig) vapor has been shown to play a pathological role in oral health and alter the oral microbiota, providing growth advantages for opportunistic pathogens. Enrichment of Staphylococcus aureus, a commensal resident in the oral cavity, correlates with the progression of periodontal disease, suggesting a role as an opportunistic pathogen. Environmental conditions, such as cigarette smoke, are known to increase S. aureus virulence, yet the role of S. aureus in periodontitis and oral preneoplasia is unknown. We exposed oral epithelial cells to e-cig aerosols and showed a dose-dependent cell viability reduction, regardless of nicotine content, in a possible attempt to repair DNA damage, as measured by pH2AX. S. aureus attachment to oral epithelial cells and bacterial biofilm formation were enhanced upon e-cig exposure, indicating an increased capacity for oral colonization. Mechanistically, e-cig aerosol exposure resulted in an immunosuppression, as determined by a reduction in IL8, IL6, and IL1β secretion by oral epithelial cells during co-culture with S. aureus. Consistent with this, e-cig vape reduced the oral epithelial cell clearance of S. aureus. Furthermore, we observed an increased expression of the inflammatory regulator COX2. This work suggests that e-cigs promote S. aureus colonization and modulate the oral inflammatory response, possibly promoting oral periodontitis and preneoplasia.
βIII-Tubulin Structural Domains Regulate Mitochondrial Network Architecture in an Isotype-Specific Manner
Authors: Parker Amelia L. , Teo Wee Siang , Brayford Simon , Moorthi Ullhas K. , Arumugam Senthil , Ferguson Charles , Parton Robert G. , McCarroll Joshua A. , Kavallaris Maria
Published: February 2022

βIII-tubulin is a neuronal microtubule protein that is aberrantly expressed in epithelial cancers. The microtubule network is implicated in regulating the architecture and dynamics of the mitochondrial network, although the isotype-specific role for β-tubulin proteins that constitute this microtubule network remains unclear. High-resolution electron microscopy revealed that manipulation of βIII-tubulin expression levels impacts the volume and shape of mitochondria. Analysis of the structural domains of the protein identifies that the C-terminal tail of βIII-tubulin, which distinguishes this protein from other β-tubulin isotypes, significantly contributes to the isotype-specific effects of βIII-tubulin on mitochondrial architecture. Mass spectrometry analysis of protein–protein interactions with β-tubulin isotypes identifies that βIII-tubulin specifically interacts with regulators of mitochondrial dynamics that may mediate these functional effects. Advanced quantitative dynamic lattice light sheet imaging of the mitochondrial network reveals that βIII-tubulin promotes a more dynamic and extended reticular mitochondrial network, and regulates mitochondrial volume. A regulatory role for the βIII-tubulin C-terminal tail in mitochondrial network dynamics and architecture has widespread implications for the maintenance of mitochondrial homeostasis in health and disease.
Noncoding RNAs in Cardiac Hypertrophy and Heart Failure
Authors: Lu Peilei , Ding Fan , Xiang Yang Kevin , Hao Liying , Zhao Meimi
Published: February 2022

Heart failure is a major global health concern. Noncoding RNAs (ncRNAs) are involved in physiological processes and in the pathogenesis of various diseases, including heart failure. ncRNAs have emerged as critical components of transcriptional regulatory pathways that govern cardiac development, stress response, signaling, and remodeling in cardiac pathology. Recently, studies of ncRNAs in cardiovascular disease have achieved significant development. Here, we discuss the roles of ncRNAs, including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs) that modulate the cardiac hypertrophy and heart failure.
Ghrelin Regulates Expression of the Transcription Factor Pax6 in Hypoxic Brain Progenitor Cells and Neurons
Authors: Stoyanova Irina , Klymenko Andrii , Willms Jeannette , Doeppner Thorsten , Tonchev Anton , Lutz David
Published: February 2022

The nature of brain impairment after hypoxia is complex and recovery harnesses different mechanisms, including neuroprotection and neurogenesis. Experimental evidence suggests that hypoxia may trigger neurogenesis postnatally by influencing the expression of a variety of transcription factors. However, the existing data are controversial. As a proof-of-principle, we subjected cultured cerebral cortex neurons, cerebellar granule neurons and organotypic cerebral cortex slices from rat brains to hypoxia and treated these cultures with the hormone ghrelin, which is well-known for its neuroprotective functions. We found that hypoxia elevated the expression levels and stimulated nuclear translocation of ghrelin’s receptor GHSR1 in the cultured neurons and the acute organotypic slices, whereas ghrelin treatment reduced the receptor expression to normoxic levels. GHSR1 expression was also increased in cerebral cortex neurons of mice with induced experimental stroke. Additional quantitative analyses of immunostainings for neuronal proliferation and differentiation markers revealed that hypoxia stimulated the proliferation of neuronal progenitors, whereas ghrelin application during the phase of recovery from hypoxia counteracted these effects. At the mechanistic level, we provide a link between the described post-ischemic phenomena and the expression of the transcription factor Pax6, an important regulator of neural progenitor cell fate. In contrast to the neurogenic niches in the brain where hypoxia is known to increase Pax6 expression, the levels of the transcription factor in cultured hypoxic cerebral cortex cells were downregulated. Moreover, the application of ghrelin to hypoxic neurons normalised the expression levels of these factors. Our findings suggest that ghrelin stimulates neurogenic factors for the protection of neurons in a GHSR1-dependent manner in non-neurogenic brain areas such as the cerebral cortex after exposure to hypoxia.
Raman Spectroscopy of V4O7 Films
Authors: Shvets Petr , Maksimova Ksenia , Goikhman Alexander
Published: February 2022

A thin film of vanadium oxide Magnéli phase V4O7 was produced using cathodic arc sputtering. X-ray diffraction, Rutherford backscattering spectrometry and Raman investigations confirmed the formation of this phase. The Raman spectrum of V4O7 differs considerably from the spectrum of another Magnéli oxide, V3O5, showing that Raman spectroscopy is an excellent tool for distinguishing between these two phases. Temperature-dependent Raman measurements revealed a significant change of the spectra near the V4O7 metal–insulator phase transition.
Comparative Study of Tribological Behavior of TiN Hard Coatings Deposited by Various PVD Deposition Techniques
Authors: Panjan Peter , Drnovšek Aljaž , Terek Pal , Miletić Aleksandar , Čekada Miha , Panjan Matjaž
Published: February 2022

In this paper, we present a comparative study of tribological properties of TiN coatings deposited by low-voltage electron beam evaporation, magnetron sputtering and cathodic arc deposition. The correlation of tribological behavior of these coatings with their intrinsic properties and friction condition was studied. The influence of surface topography and the surrounding atmosphere was analyzed in more detail. We limited ourselves to the investigation of tribological processes that take place in the initial phase of the sliding test (the first 1000 cycles). A significant difference in the initial phase of the sliding test of three types of TiN coatings was observed. We found that nodular defects on the coating surface have an important role in this stage of the sliding test. The tribological response of TiN coatings, prepared by cathodic arc deposition, is also affected by the metal droplets on the coating surface, as well as those incorporated in the coating itself. Namely, the soft metal droplets increase the adhesion component of friction. The wear rates increased with the surface roughness of TiN coatings, the most for coatings prepared by cathodic arc deposition. The influences of post-polishing of the coating and the surrounding atmosphere were also investigated. The sliding tests on different types of TiN coatings were conducted in ambient air, oxygen and nitrogen. While oxygen promotes tribo-chemical reactions at the contact surface of the coating, nitrogen suppresses them. We found that the wear rate measured in ambient air, compared with that in an oxygen atmosphere, was lower. The difference is probably due to the influence of humidity in the ambient air. On the other hand, wear rates measured in a nitrogen atmosphere were much lower in comparison with those measured in an oxygen or ambient air atmosphere.
Research on the Mechanism of Anti-Stone-Damage Automotive Coating Based on Computational Fluid Dynamics Method
Authors: Xiao Guoquan
Published: February 2022

Damage to the body or critical components caused by stone strikes to automotive coatings affects the safety of the car. More and more attention are being paid to the study of the anti-stone-damage mechanism of coatings. Firstly, a computational fluid dynamics (CFD) model was established based on a single-impact anti-stone tester, and the simulation results show the following: (a) At 90° incidence, the air pressure of the stone impact suddenly and rapidly increases when the semicircular surface projectile velocity is greater than 30 m/s. The air pressure of the conical projectile stone at the distance of 0.3 and 0.15 mm suddenly increases at the projectile velocity of 10 and 20 m/s, respectively. (b) When the projectile velocity exceeds 15 m/s, the pressure of the semicircular surface projectile suddenly increases. (c) When the speed is less than 20 m/s, the shedding area decreases with the increase in the speed, while the stone impact pressure increases with the increase in the speed. Then, the simulation results of the incident velocity of 40 m/s show that the working pressure is 195.48 kPa, and the impact force is 8142.56 N. Finally, the relationship between the impact force and the driving air flow pressure was obtained according to the DIN 55996-1 standard stone impact resistance test and compared with the simulation results, showing that trend of the impact force increasing with the increase in driving air flow pressure is consistent, the simulation results are about 1.40 times the experimental results, and the simulation results are large and can be considered by increasing the test correction coefficient adjustment. Based on the CFD method, research on the mechanism of anti-stone-damage automotive coating can greatly reduce the number of stone strike instrument experiments, shorten the cycle, and reduce the research and development costs.
Color Analysis of Metal Ceramic Restorations Fabricated from Different Dental Laboratories
Authors: Aljamhan Abdullah Saleh , Habib Syed Rashid , Khan Aleshba Saba , Javed Muhammad Qasim , Bhatti Usman Anwer , Zafar Muhammad Sohail
Published: February 2022

Objective: The aim of the present in vitro study was to explore the differences in color matching of most frequently used shades for metal ceramic dental restorations fabricated by different laboratories. Materials and Methods: A total sample size of 180 discs of metal ceramic were prepared by six laboratories (labs): each lab prepared 30 samples, 10 for each shade (A1, B1, and C1). Instructions for standardization were provided. A spectrophotometer and CIE LAB system was used to find ΔE value for difference between shades observed by spectrophotometer. For statistical analysis, ANOVA and post hoc Tukey tests were used. Significance level was <0.05. Results: Significant variation (p = 0.000) was observed among the labs for ΔE. The maximum value for ΔE in shade A1 was 77.0 ± 0.065 and minimum value for ΔE noted for shade A1 was 69.19 ± 1.39. The values of ΔE for shade B1 were maximum 80.14 ± 0.16 and minimum 68.38 ± 0.40 and the values of ΔE for shade C1 were maximum 80.16 ± 0.14 and minimum 71.62 ± 1.23. Twelve out of 15 comparisons between labs for shade A1, 14 of 15 comparisons for shade B1, and 11 of 15 comparisons for shade C1 showed significant difference (p = 0.000). Conclusions: Similar tooth shades (A1, B1, and C1) when duplicated by different labs exhibited highly significant color variations.
Titanium Oxide Coatings Deposited on MnZn Ferrite by a Molten Salt Reaction
Authors: Wang Hongyang , Cui Erchuang , Wang Chengbin , Yan Long , Zhang Wei , Yu Guojun
Published: February 2022

Using molten salt reactions, Ti oxide coatings were successfully deposited on MnZn ferrite in a NaCl–KCl–K2TiF6 melt. The film formed on the surface of MnZn ferrite had a thickness of up to 20 μm and was composed of Ti oxides. The electric conductivity of the coatings was contributed to by the component Ti2O3 and depended on the concentration of Ti2O3 in the film. When the Ti2O3 content in the film increased, the surface resistance dropped to the order of 10−1 Ω/□. The conductive film had the potential to be welded to the metal electrode, thus confirming that the molten salt reaction method could be used as a pre-treatment to realize the metallization of MnZn ferrite.
Review on the Development and Application of Directional Water Transport Textile Materials
Authors: Xiao Ya-Qian , Kan Chi-Wai
Published: February 2022

Moisture (sweat) management in textile products is crucial to regulate human thermo-physiological comfort. Traditional hydrophilic textiles, such as cotton, can absorb sweat, but they retain it, leading to undesired wet adhesion sensation and even excessive cooling. To address such issues, the development of functional textiles with directional water transport (DWT) has garnered great deal of interest. DWT textile materials can realize directional water transport and prevent water penetration in the reverse direction, which is a great application for sweat release in daily life. In this review article, the mechanism of directional water transport is analyzed. Then, three key methods to achieve DWT performance are reviewed, including the design of the fabric structure, surface modification and electrospinning. In addition, the applications of DWT textile materials in functional clothing, electronic textiles, and wound dressing are introduced. Finally, the challenges and future development trends of DWT textile materials in the textile field are discussed.
Composite Films of Nanofibrillated Cellulose with Sepiolite: Effect of Preparation Strategy
Authors: Alves Luís , Ramos Ana , Rasteiro Maria G. , Vitorino Carla , Ferraz Eduardo , Ferreira Paulo J. T. , Puertas Maria L. , Gamelas José A. F.
Published: February 2022

Cellulose nanofibrils (CNFs) are nanomaterials with promising properties to be used in food packaging and printed electronics, thus being logical substitutes to petroleum-based polymers, specifically plastics. CNFs can be combined with other materials, such as clay minerals, to form composites, which are environmentally friendly materials, with acceptable costs and without compromising the final properties of the composite material. To produce composite films, two strategies can be used: solvent casting and filtration followed by hot pressing. The first approach is the simplest way to produce films, but the obtained films may present some limitations. In the present work, CNFs produced using enzymatic or TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidation pretreatments, followed by high-pressure homogenization, or only by mechanical treatment (homogenization), were used to produce films by both the available procedures. The films obtained by filtration + hot pressing presented higher tensile strength and Young’s modulus compared with those obtained by solvent casting. In general, a decrease in the values of these mechanical properties of the films and a decrease in elongation at break, with the addition of sepiolite, were also observed. However, for the TEMPO CNF-based films, an improvement in tensile strength could be observed for 10% of the sepiolite content. Furthermore, the time necessary to produce films was largely reduced by employing the filtration procedure. Finally, the water vapour barrier properties of the films obtained by filtration are comparable to the literature values of net CNF films. Thus, this technique demonstrates to be the most suitable to produce CNF-based composite films in a fast way and with improved mechanical properties and suitable gas barrier properties.
Learning from Peer Mistakes: Collaborative UML-Based ITS with Peer Feedback Evaluation
Authors: Abrejo Sehrish , Kazi Hameedullah , Rahman Mutee U. , Baloch Ahsanullah , Baig Amber
Published: February 2022

Collaborative Intelligent Tutoring Systems (ITSs) use peer tutor assessment to give feedback to students in solving problems. Through this feedback, the students reflect on their thinking and try to improve it when they get similar questions. The accuracy of the feedback given by the peers is important because this helps students to improve their learning skills. If the student acting as a peer tutor is unclear about the topic, then they will probably provide incorrect feedback. There have been very few attempts in the literature that provide limited support to improve the accuracy and relevancy of peer feedback. This paper presents a collaborative ITS to teach Unified Modeling Language (UML), which is designed in such a way that it can detect erroneous feedback before it is delivered to the student. The evaluations conducted in this study indicate that receiving and sending incorrect feedback have negative impact on students’ learning skills. Furthermore, the results also show that the experimental group with peer feedback evaluation has significant learning gains compared to the control group.
Attention Mechanism Guided Deep Regression Model for Acne Severity Grading
Authors: Alzahrani Saeed , Al-Bander Baidaa , Al-Nuaimy Waleed
Published: February 2022

Acne vulgaris is the common form of acne that primarily affects adolescents, characterised by an eruption of inflammatory and/or non-inflammatory skin lesions. Accurate evaluation and severity grading of acne play a significant role in precise treatment for patients. Manual acne examination is typically conducted by dermatologists through visual inspection of the patient skin and counting the number of acne lesions. However, this task costs time and requires excessive effort by dermatologists. This paper presents automated acne counting and severity grading method from facial images. To this end, we develop a multi-scale dilated fully convolutional regressor for density map generation integrated with an attention mechanism. The proposed fully convolutional regressor module adapts UNet with dilated convolution filters to systematically aggregate multi-scale contextual information for density maps generation. We incorporate an attention mechanism represented by prior knowledge of bounding boxes generated by Faster R-CNN into the regressor model. This attention mechanism guides the regressor model on where to look for the acne lesions by locating the most salient features related to the understudied acne lesions, therefore improving its robustness to diverse facial acne lesion distributions in sparse and dense regions. Finally, integrating over the generated density maps yields the count of acne lesions within an image, and subsequently the acne count indicates the level of acne severity. The obtained results demonstrate improved performance compared to the state-of-the-art methods in terms of regression and classification metrics. The developed computer-based diagnosis tool would greatly benefit and support automated acne lesion severity grading, significantly reducing the manual assessment and evaluation workload.
Strongly Confining Light with Air-Mode Cavities in Inverse Rod-Connected Diamond Photonic Crystals
Authors: Taverne Mike P. C. , Ho Ying-Lung D. , Rarity John G.
Published: February 2022

Three-dimensional dielectric optical crystals with a high index show a complete photonic bandgap (PBG), blocking light propagation in all directions. We show that this bandgap can be used to trap light in low-index defect cavities, leading to strongly enhanced local fields. We compute the band structure and optimize the bandgap of an inverse 3D rod-connected diamond (RCD) structure, using the plane-wave expansion (PWE) method. Selecting a structure with wide bandgap parameters, we then add air defects at the center of one of the high-index rods of the crystal and study the resulting cavity modes by exciting them with a broadband dipole source, using the finite-difference time-domain (FDTD) method. Various defect shapes were studied and showed extremely small normalized mode volumes (Veff) with long cavity storage times (quality factor Q). For an air-filled spherical cavity of radius 0.1 unit-cell, a record small-cavity mode volume of Veff~2.2 × 10−3 cubic wavelengths was obtained with Q~3.5 × 106.
Potential of Recycled Silicon and Silicon-Based Thermoelectrics for Power Generation
Authors: Duran Solco , Zhang Danwei , Lim Wei , Cao Jing , Liu Hongfei , Zhu Qiang , Tan Chee , Xu Jianwei , Loh Xian , Suwardi Ady
Published: February 2022

Thermoelectrics can convert waste heat to electricity and vice versa. The energy conversion efficiency depends on materials figure of merit, zT, and Carnot efficiency. Due to the higher Carnot efficiency at a higher temperature gradient, high-temperature thermoelectrics are attractive for waste heat recycling. Among high-temperature thermoelectrics, silicon-based compounds are attractive due to the confluence of light weight, high abundance, and low cost. Adding to their attractiveness is the generally defect-tolerant nature of thermoelectrics. This makes them a suitable target application for recycled silicon waste from electronic (e-waste) and solar cell waste. In this review, we summarize the usage of high-temperature thermoelectric generators (TEGs) in applications such as commercial aviation and space voyages. Special emphasis is placed on silicon-based compounds, which include some recent works on recycled silicon and their thermoelectric properties. Besides materials design, device designing considerations to further maximize the energy conversion efficiencies are also discussed. The insights derived from this review can be used to guide sustainable recycling of e-waste into thermoelectrics for power harvesting.
Hydrothermally Grown ZnO Nanostructures for Water Purification via Photocatalysis
Authors: Le Pivert Marie , Martin Nathan , Leprince-Wang Yamin
Published: February 2022

Semiconductor-based photocatalysis is a well-known and efficient process for achieving water depollution with very limited rejects in the environment. Zinc oxide (ZnO), as a wide-bandgap metallic oxide, is an excellent photocatalyst, able to mineralize a large scale of organic pollutants in water, under UV irradiation, that can be enlarged to visible range by doping nontoxic elements such as Ag and Fe. With high surface/volume ratio, the ZnO nanostructures have been shown to be prominent photocatalyst candidates with enhanced photocatalytic efficiency, owing to their being low-cost, non-toxic, and able to be produced with easy and controllable synthesis. Thus, ZnO nanostructures-based photocatalysis can be considered as an eco-friendly and sustainable process. This paper presents the photocatalytic activity of ZnO nanostructures (NSs) grown on different substrates. The photocatalysis has been carried out both under classic mode and microfluidic mode. All tests show the notable photocatalytic efficiency of ZnO NSs with remarkable results obtained from a ZnO-NSs-integrated microfluidic reactor, which exhibited an important enhancement of photocatalytic activity by drastically reducing the photodegradation time. UV-visible spectrometry and high-performance liquid chromatography, coupled with mass spectrometry (HPLC-MS), are simultaneously used to follow real-time information, revealing both the photodegradation efficiency and the degradation mechanism of the organic dye methylene blue.
Influence of the Reaction Conditions in the Crystal Structures of Zn(II) and Ni(II) Coordination Compounds with a Dissymmetric Bis(Thiosemicarbazone) Ligand
Authors: Alonso Luis , Burón Rodrigo , López-Torres Elena , Mendiola Maria Antonia
Published: February 2022

The new ligand HMeATSM, derived from condensation of 2-3-butanedione with 4-methyl-3-thiosemicarbazide and 2,4-dimethyl-3-thiosemicarbazide, has been synthesized. Its reactivity with nickel(II) and zinc(II) nitrates was explored and the resulting complexes were thoroughly characterized by elemental analysis, conductivity, mass spectrometry, IR, 1H and 13C NMR spectroscopies and their structures were confirmed by single-crystal X-ray diffraction. The results showed that the complex [Ni(MeATSM)]NO3 1 is formed under every reaction condition. In contrast, the reaction with zinc(II) nitrate depends on the temperature and the presence of LiOH·H2O, leading to the obtaining of complexes [Zn(MeATSM)(OH2)](NO3) 2 and [Zn(Me2TS)2(OH2)](NO3)2 3. The crystal structures of complexes 1 and 2 show that the dissymmetric ligand acts as a N2S2 tetradentate monoanionic ligand. The structural preferences of the metals also determine the structure of the complexes: whereas nickel(II) is in a square-planar environment, the zinc atom prefers a distorted square-base pyramid geometry imposed by the coordination mode and the planarity of the bis(thiosemicarbazone) ligand. In contrast, in complex 3, containing two bidentate Me2TS ligands, the Zn(II) is in a trigonal bipyramid arrangement. In all the complexes, the nitrate ion is not coordinated to the metal and acts as a counterion.
A Synthetic Analog of the Mineral Ivanyukite: Sorption Behavior to Lead Cations
Authors: Samburov Gleb O. , Kalashnikova Galina O. , Panikorovskii Taras L. , Bocharov Vladimir N. , Kasikov Aleksandr , Selivanova Ekaterina , Bazai Ayya V. , Bernadskaya Daria , Yakovenchuk Viktor N. , Krivovichev Sergey V.
Published: February 2022

The production of electrolytic nickel includes the stage of leaching of captured firing nickel matte dust. The solutions formed during this process contain considerable amounts of Pb, which is difficult to extraction due to its low concentration upon the high-salt background. The sorption of lead from model solutions with various compositions by synthetic and natural titanosilicate sorbents (synthetic ivanyukite-Na-T (SIV), ivanyukite-Na-T, and AM-4) have been investigated. The maximal sorption capacity of Pb is up to 400 mg/g and was demonstrated by synthetic ivanyukite In solutions with the high content of Cl− (20 g/L), extraction was observed only with a high amount of Na (150 g/L). Molecular mechanisms and kinetics of lead incorporation into ivanyukite were studied by the combination of single-crystal and powder X-ray diffraction, microprobe analysis, and Raman spectroscopy. Incorporation of lead into natural ivanyukite-Na-T with the R3m symmetry by the substitution 2Na+ + 2O2− ↔ Pb2+ + □ + 2OH− leds to its transformation into the cubic P−43m Pb-exchanged form with the empirical formulae Pb1.26[Ti4O2.52(OH)1.48(SiO4)3]·3.32(H2O).
Life from a Snowflake: Diversity and Adaptation of Cold-Loving Bacteria among Ice Crystals
Authors: Rizzo Carmen , Lo Giudice Angelina
Published: February 2022

Incredible as it is, researchers have now the awareness that even the most extreme environment includes special habitats that host several forms of life. Cold environments cover different compartments of the cryosphere, as sea and freshwater ice, glaciers, snow, and permafrost. Although these are very particular environmental compartments in which various stressors coexist (i.e., freeze–thaw cycles, scarce water availability, irradiance conditions, and poorness of nutrients), diverse specialized microbial communities are harbored. This raises many intriguing questions, many of which are still unresolved. For instance, a challenging focus is to understand if microorganisms survive trapped frozen among ice crystals for long periods of time or if they indeed remain metabolically active. Likewise, a look at their site-specific diversity and at their putative geochemical activity is demanded, as well as at the equally interesting microbial activity at subzero temperatures. The production of special molecules such as strategy of adaptations, cryoprotectants, and ice crystal-controlling molecules is even more intriguing. This paper aims at reviewing all these aspects with the intent of providing a thorough overview of the main contributors in investigating the microbial life in the cryosphere, touching on the themes of diversity, adaptation, and metabolic potential.
Mini-LED Backlight Technology Progress for Liquid Crystal Display
Authors: Gao Zhiwen , Ning Honglong , Yao Rihui , Xu Wei , Zou Wenxin , Guo Chenxiao , Luo Dongxiang , Xu Hengrong , Xiao Junlin
Published: February 2022

As consumers pursue higher display quality, Mini-LED backlight technology has become the focus of research in the current display field. With its size advantage (100–200 μm), it can achieve one-thousand-level divisional dimming, and it can also be combined with quantum dot technology to greatly improve the contrast, color gamut, dark state and other element of the display performance of LCD displays. Mini-LED backlight technology is undoubtedly the most ideal solution to realize a highly dynamic range display of LCD displays, and has been widely commercialized in many fields such as TVs, tablet computers, notebook computers, and car monitors. This review mainly introduces the efforts made by researchers to eliminate the halo effect, thinning of the backlight module and reducing the backlight power consumption. The application of quantum dot technology in backlight is also presented. We predict that the number of Mini-LED backlight partitions is expected to reach a level of more than 3000 in the future, further utilizing the advantages of the small size in local dimming, but it will also inevitably be challenged by some issues such as power consumption and heat dissipation.
Interaction between a Screw Dislocation and Two Unequal Interface Cracks Emanating from an Elliptical Hole in One Dimensional Hexagonal Piezoelectric Quasicrystal Bi-Material
Authors: Pi Jiandong , Zhao Yue , Li Lianhe
Published: February 2022

Utilizing conformal transformation, a screw dislocation interacting with two unequal interface cracks emanating from an elliptical hole in a one-dimensional hexagonal piezoelectric quasicrystal bi-material was studied. The analytic expressions of the interface stresses, electric displacement and stress intensity factors were obtained. With the help of the generalization of the Peach–Koehler formula for quasicrystals, the image force acting on the dislocation due to the presence of the interface was then determined. Numerical examples are given to show the effects of the coupling elastic constants of the phonon field and phason field on the field intensity factors.
A Real-Life Reproducibility Assessment for NMR Metabolomics
Authors: Stavarache Cristina , Nicolescu Alina , Duduianu Cătălin , Ailiesei Gabriela Liliana , Balan-Porcăraşu Mihaela , Cristea Mihaela , Macsim Ana-Maria , Popa Oana , Stavarache Carmen , Hîrtopeanu Anca , Barbeş Lucica , Stan Raluca , Iovu Horia , Deleanu Calin
Published: February 2022

Nuclear magnetic resonance (NMR) metabolomics is currently popular enough to attract both specialized and non-specialized NMR groups involving both analytical trained personnel and newcomers, including undergraduate students. Recent interlaboratory studies performed by established NMR metabolomics groups demonstrated high reproducibility of the state-of-the-art NMR equipment and SOPs. There is, however, no assessment of NMR reproducibility when mixing both analytical experts and newcomers. An interlaboratory assessment of NMR quantitation reproducibility was performed using two NMR instruments belonging to different laboratories and involving several operators with different backgrounds and metabolomics expertise for the purpose of assessing the limiting factors for data reproducibility in a multipurpose NMR environment. The variability induced by the operator, automatic pipettes, NMR tubes and NMR instruments was evaluated in order to assess the limiting factors for quantitation reproducibility. The results estimated the expected reproducibility data in a real-life multipurpose NMR laboratory to a maximum 4% variability, demonstrating that the current NMR equipment and SOPs may compensate some of the operator-induced variability.
A User-Friendly Approach for Routine Histopathological and Morphometric Analysis of Skeletal Muscle Using CellProfiler Software
Authors: Laghi Valerio , Ricci Valentina , De Santa Francesca , Torcinaro Alessio
Published: February 2022

Adult skeletal muscle is capable of active and efficient differentiation in the event of injury in both physiological and pathological conditions, such as in Duchenne muscular dystrophy (DMD). DMD is characterized by different features, such as continuous cycles of degeneration/regeneration, fiber heterogeneity, chronic inflammation and fibrosis. A well-defined and standardized approach for histological and morphometric analysis of muscle samples is necessary in order to measure and quantify specific regenerative parameters in myopathies. Indeed, non-automatic methods are time-consuming and prone to error. Here, we describe a simple automatized computational approach to quantify muscle parameters with specific pipelines to be run by CellProfiler software in an open-source and well-defined fashion. Our pipelines consist of running image-processing modules in CellProfiler with the aim of quantifying different histopathological muscle hallmarks in mdx mice compared to their wild-type littermates. Specifically, we quantified the minimum Feret diameter, centrally nucleated fibers and the number of macrophages, starting from multiple images. Finally, for extracellular matrix quantification, we used Sirius red staining. Collectively, we developed reliable and easy-to-use pipelines that automatically measure parameters of muscle histology, useful for research in myobiology. These findings should simplify and shorten the time needed for the quantification of muscle histological properties, avoiding challenging manual procedures.
Associations between Patient Report of Pain and Intervertebral Foramina Changes Visible on Axial-Loaded Lumbar Magnetic Resonance Imaging
Authors: Lorenc Tomasz , Gołębiowski Marek , Syganiec Dariusz , Glinkowski Wojciech M.
Published: February 2022

The intervertebral foramen may influence spinal nerve roots and, therefore, be related to the corresponding dermatomal pain. In vivo evaluation of the intervertebral foramen–dermatome relationship is essential for understanding low back pain (LBP) pathophysiology. The study aimed to correlate the lumbar MRI unloaded-loaded foraminal area changes with dermatomal pain in the patient’s pain drawings. Dynamic changes of the dermatomal pain distribution related to the intervertebral foramen area changes between quantitative conventional supine MRI (unloaded MRI) and axial-loading MRI (alMRI) were analyzed. The MRI axial-loading intervertebral foramen area changes were observed, and the most significant effect of reducing the foraminal area (−6.9%) was reported at levels of L2–L3. The incidence of pain in the dermatomes increases linearly with the spine level, from 15.6% at L1 to 63.3% at L5 on the right and from 18.9% at L1 to 76.7% at L5 on the left. No statistically significant effect of changes in the intervertebral foramen area on the odds of pain along the respective dermatomes was confirmed. Changes in the foraminal area were observed between the unloaded and loaded phases, but differences in area changes between foramen assigned to painful dermatomes and foramen assigned to non-painful dermatomes were not significant.
Percutaneous Thermal Ablation Therapy of Hepatocellular Carcinoma (HCC): Microwave Ablation (MWA) versus Laser-Induced Thermotherapy (LITT)
Authors: Adwan Hamzah , Vogl Thomas J. , Balaban Ümniye , Nour-Eldin Nour-Eldin Abdelrehim
Published: February 2022

The purpose of this study is to compare the efficacy and safety of microwave ablation (MWA) versus laser-induced thermotherapy (LITT) as a local treatment for hepatocellular carcinoma (HCC,) with regard to therapy response, survival rates, and complication rates as measurable outcomes. This retrospective study included 250 patients (52 females and 198 males; mean age: 66 ± 10 years) with 435 tumors that were treated by MWA and 53 patients (12 females and 41 males; mean age: 67.5 ± 8 years) with 75 tumors that were treated by LITT. Tumor response was evaluated using CEMRI (contrast-enhanced magnetic resonance imaging). Overall, 445 MWA sessions and 76 LITT sessions were performed. The rate of local tumor progression (LTP) and the rate of intrahepatic distant recurrence (IDR) were 6% (15/250) and 46% (115/250) in the MWA-group and 3.8% (2/53) and 64.2% (34/53) in the LITT-group, respectively. The 1-, 3-, and 5-year overall survival (OS) rates calculated from the date of diagnosis were 94.3%, 65.4%, and 49.1% in the MWA-group and 96.2%, 54.7%, and 30.2% in the LITT-group, respectively (p-value: 0.002). The 1-, 2-, and 3-year disease-free survival (DFS) rates were 45.9%, 30.6%, and 24.8% in the MWA-group and 54.7%, 30.2%, and 17% in the LITT-group, respectively (p-value: 0.719). Initial complete ablation rate was 97.7% (425/435) in the MWA-group and 98.7% (74/75) in the LITT-group (p-value > 0.99). The overall complication rate was 2.9% (13/445) in the MWA-group and 7.9% (6/76) in the LITT-group (p-value: 0.045). Based on the results, MWA and LITT thermal ablation techniques are well-tolerated, effective, and safe for the local treatment of HCC. However, MWA is recommended over LITT for the treatment of HCC, since the patients in the MWA-group had higher survival rates.
Kabat Rehabilitation in Facial Nerve Palsy after Parotid Gland Tumor Surgery: A Case-Control Study
Authors: Boschetti Ciro Emiliano , Lo Giudice Giorgio , Spuntarelli Chiara , Apice Carmine , Rauso Raffaele , Santagata Mario , Tartaro Gianpaolo , Colella Giuseppe
Published: February 2022

Temporary facial nerve palsy after parotid tumor surgery ranges from 14 to 65%, depending on surgery, tumor type, and subsite. The study aimed to evaluate the role of Kabat physical rehabilitation in the outcomes of patients affected by severe facial nerve palsy following parotid gland surgery. The results and clinical data of two groups, Kabat and non-Kabat (control), were statistically compared. Descriptive statistics, the multiple linear regression model, difference in difference approach, and the generalized linear model were used. F-Test, Chi-square test, McFadden R-squared, and adjusted R-squared were used to assess the significance. The results showed that the House–Brackmann (HB) stage of patients who had physiotherapy performed were lower than the control group. The decrease of HB staging in the Kabat group at 3 months was −0.71 on average, thus the probability of having a high HB stage decreased by about 13% using Kabat therapy. The results are statistically significant, and indicated that when the Kabat rehabilitation protocol is performed, mainly in the cases of a high-grade HB score, the patients showed a better and faster improvement in postoperative facial nerve palsy.
Coexistence of Thumb Aplasia and Cleft Lip and Alveolus with Aortopulmonary Window—A Tip for Prenatal Diagnostics for Rare Heart Anomalies
Authors: Kasielska-Trojan Anna , Święchowicz Barbara , Antoszewski Bogusław
Published: February 2022

Multiple congenital anomaly syndromes pose a challenge to neonatologists, as many anomalies may indicate cryptogenic malformations or disorders. Aortopulmonary window (APW) is a very rare congenital heart disease (CHD) and causes many difficulties in prenatal diagnostics. In this report, we describe a case of a female patient with multiple rare congenital malformations: aortopulmonary window, right thumb aplasia, facial nerve palsy and cleft lip and alveolus. None of the malformations were diagnosed prenatally. A long-term follow-up (40 years) is presented. The presence of certain defects (thumb aplasia) may indicate the need for a careful fetal examination extended by a fetal ECHO performed in a reference center of prenatal cardiology. The coexistence or syndromic character of the presented malformations should be verified in future if more such cases are described.
Convolutional Neural Network-Based Automatic Analysis of Chest Radiographs for the Detection of COVID-19 Pneumonia: A Prioritizing Tool in the Emergency Department, Phase I Study and Preliminary “Real Life” Results
Authors: Tricarico Davide , Calandri Marco , Barba Matteo , Piatti Clara , Geninatti Carlotta , Basile Domenico , Gatti Marco , Melis Massimiliano , Veltri Andrea
Published: February 2022

The aim of our study is the development of an automatic tool for the prioritization of COVID-19 diagnostic workflow in the emergency department by analyzing chest X-rays (CXRs). The Convolutional Neural Network (CNN)-based method we propose has been tested retrospectively on a single-center set of 542 CXRs evaluated by experienced radiologists. The SARS-CoV-2 positive dataset (n = 234) consists of CXRs collected between March and April 2020, with the COVID-19 infection being confirmed by an RT-PCR test within 24 h. The SARS-CoV-2 negative dataset (n = 308) includes CXRs from 2019, therefore prior to the pandemic. For each image, the CNN computes COVID-19 risk indicators, identifying COVID-19 cases and prioritizing the urgent ones. After installing the software into the hospital RIS, a preliminary comparison between local daily COVID-19 cases and predicted risk indicators for 2918 CXRs in the same period was performed. Significant improvements were obtained for both prioritization and identification using the proposed method. Mean Average Precision (MAP) increased (p < 1.21 × 10−21 from 43.79% with random sorting to 71.75% with our method. CNN sensitivity was 78.23%, higher than radiologists’ 61.1%; specificity was 64.20%. In the real-life setting, this method had a correlation of 0.873. The proposed CNN-based system effectively prioritizes CXRs according to COVID-19 risk in an experimental setting; preliminary real-life results revealed high concordance with local pandemic incidence.
“Modified Schirmer Test” as an Objective Measurement for Vaginal Dryness: A Prospective Cohort Study
Authors: Gabrieli Dana , Suissa-Cohen Yael , Jaber Sireen , Lev-Sagie Ahinoam
Published: February 2022

None of the currently available parameters allow for a direct and objective measurement of vaginal moisture. We used a calibrated filter paper strip as a measurement tool for the quantification of vaginal fluid, in a similar manner as the ophthalmic “Schirmer test” (used for eye moisture measurement). The study aimed to evaluate the validity of this new, objective tool, to measure vaginal moisture. We compared vaginal moisture measurements using the “modified Schirmer test” in symptomatic women with genitourinary syndrome of menopause to those of women without vaginal dryness. The mean “modified Schirmer test” measurement in the control group was 21.7 mm compared to 3.3 mm in the study group, yielding a statistically significant difference (p < 0.001). Strong correlations were found between “modified Schirmer test” measurements and pH (correlation coefficient −0.714), Vaginal Health Index [VHI (0.775)], and Visual Analogue Score (VAS) of dryness during intercourse (−0.821). Our findings suggest that the “modified Schirmer test” can be used as an objective measurement for the assessment of vaginal fluid level. This test may also prove useful for evaluation of non-hormonal treatments aimed to treat vaginal dryness.