bluuebunny/crossref_metadata_2025 huggingface dataset:
INDONESIAN EFL TEACHERS’ PERCEPTIONS TOWARD ICT INTEGRATION FOR LANGUAGE TEACHING IN REMOTE AREAS
Authors: Muhaji Uun , Muzammil Lasim , Andy
Published: January 2025

Considering the challenges in integrating Information and Communication Technology (ICT) into language teaching in Indonesia’s remote areas, the perspectives of English as a Foreign Language (EFL) teachers on this development have not been thoroughly investigated. This study aims to address questions regarding the extent of their awareness of ICT advancements, their integration of ICT into English language teaching (ELT), and their concerns about their ICT skills and knowledge. Data were collected through an online survey of 50 EFL teachers working in remote areas of West Kalimantan and East Nusa Tenggara. The survey included seven closed-ended and four open-ended questions focusing on their attitudes toward ICT development in language teaching and their adoption of ICT practices. The results indicate that the participants hold positive perceptions of ICT development in language teaching and, despite various limitations, make efforts to integrate ICT into their teaching practices. However, they also expressed significant concerns about their skills and knowledge of ICT. These findings highlight the need for supporting facilities and targeted training to enhance ICT integration in language teaching for EFL teachers in remote areas.
ANALISIS EFEKTIVITAS APLIKASI MYITS THESIS MENGGUNAKAN CONFIRMATORY FACTOR ANALYSIS UNTUK PENINGKATAN LAYANAN PENYELENGGARAAN UJIAN PADA PROGRAM DOKTOR ILMU KOMPUTER
Authors: Ambarwati Lina - , D'layla Adifa Widyadhani Chanda , Saikhu Ahmad
Published: January 2025

In the global era, Information Systems in higher education institutions are a must. Institut Teknologi Sepuluh Nopember (ITS) continuously makes efforts to develop information systems for various academic and non-academic services for the lecturer and students. One of the academic services developed at ITS is myITS Thesis application. myITS Thesis is one of the applications prepared for the management of scholars Final Project. The Computer Science Doctoral Program (PDIK) has implemented the application for examination services including qualification hearings progress, seminars, and closed dissertation hearings. This study aims to measure the effectiveness of using the myITS Thesis application in managing PDIK dissertation research services to stakeholders, especially PDIK scholars. Measurements were carried out by surveying through questionnaires with PDIK participants who used the application in the 2023/2024 academic year. The effectiveness of the application is measured through five factors, namely System Quality (KS), Information Quality(KI), System Use(PS), User Satisfaction(KP), and Individual Impact(DI). The five factors are measured through 30 Question Indicators to 55 respondents. The results of the questionnaire survey were processed using descriptive analysis and CFA modeling. CFA is used to measure validity and reliability through Standardized Loading Factor (SLF), Cronbach Alpha (CA), and Composite Reliability (CR) values. It is concluded from the modeling results based on validity and reliability measurements that the KS factor is valid with CA value=0.931 and reliable with CR value=0.73, the KI factor is valid with CA value=0.923 and reliable with CR value=0.706, the PS factor is valid with CA value=0.95 and reliable with CR value=0.734. While the KP factor is valid with CA value=0.972 and reliable with CR value= 0.814. Therefore, the myITS Thesis application has been quite effective in improving exam administration services.Keywords: questionnaire, descriptive analysis, PDIK, myITS Thesis, CFA modeling
Plan de Negocios para la comercialización de chocolate a base de cacao y quinua apto para diabéticos vía online en lima metropolitana
Authors:   , Aranda Barrera Mariana Vanessa , Oyola Garcia Mary Estefany , Pahuacho Torres Elena Joice
Published: January 2025

Nuestro plan de negocios consiste en la creación de una empresa de venta online de chocolate a base de cacao orgánico proveniente de nuestra selva peruana y quinua andina, además se encuentra endulzado naturalmente con fruto del monje, nuestra idea de negocio es ofrecerlo en el mercado de la ciudad de Lima, el objetivo es establecer las estrategias claves para el lanzamiento y crecimiento de CHOCONÚA en presentación de tabletas semi cortadas para que puedan consumirlo en porciones. Este producto es creado en respuesta a una necesidad identificada en el mercado local, después de la realización de un análisis e investigación de estudios y censos publicados en el mercado de Lima metropolitana. Entre los principales hallazgos obtenidos, determinamos que existe un público objetivo en los segmentos socioeconómicos A y B conformados por personas entre 25 y 55 años que desean encontrar en el mercado un chocolate saludable que se pueda disfrutar como un snack sin la preocupación por el alto contenido de azúcar que este pueda tener.
A raíz de este hallazgo hemos planteado estrategias, utilizando herramientas como análisis PESTEL y Las 5 Fuerzas de Porter. Además de entrevistas de profundidad y encuestas, lo cual nos ha permitido entender mejor las necesidades y preferencias de nuestros potenciales clientes y así, lograr diseñar un producto y un modelo de negocio alineados estrechamente con las necesidades identificadas.
En el frente del marketing, hemos desarrollado una serie de estrategias combinadas para lograr la diferenciación de la competencia que hoy existe en el mercado, y posicionar nuestra marca de forma eficaz en el mercado objetivo. Hemos desarrollado la estrategia para la venta online, considerando las tendencias que van hacia el comercio electrónico.
Administración local de prostaglandinas para acelerar el movimiento ortodóntico - Revisión de la literatura
Authors:   , Martinez Aldave Alejandro Javier
Published: January 2025

Objetivo: La búsqueda de optimizar el movimiento dentario y reducir el tiempo de tratamiento es de gran interés para el ortodoncista, innovando con nuevos materiales y procedimientos que permitan alcanzar los objetivos de tratamiento en un corto plazo. La administración de prostaglandinas ha sido estudiada y se ha comprobado su efecto acelerador del movimiento dentario al ser administradas localmente. El objetivo de este estudio fue evaluar los últimos alcances sobre los efectos de la administración local de prostaglandinas para acelerar el movimiento dentario. Metodología: Se realizó una búsqueda en cuatro bases de datos (PubMed, SciELO, Cochrane, Google Scholar) y tres revistas especializadas (AJODO, Angle Orthodontics, Journal of Clinical Orthodontics) hasta agosto 2023. La calidad metodológica de los estudios fue analizada usando PRISMA checklist. Resultados: El estudio contó con 14 artículos. La administración local de prostaglandinas acelera la velocidad del movimiento dentario e incrementa el número de osteoclastos. Se ha reportado evidencia de reabsorción radicular en relación con altas dosis de prostaglandinas por la vía submucosal, y una reducción de la reabsorción radicular administrando prostaglandinas con gluconato de calcio. La administración local de prostaglandinas por la vía submucosal produce un dolor leve a moderado y se recomienda su inoculación junto a un anestésico local, sin embargo, la administración oral del compuesto análogo Misoprostol, no produce dolor ni evidencia de reabsorción radicular. Conclusiones: No se ha encontrado evidencia científica suficiente que sustente la administración local de prostaglandinas como un método seguro para acelerar el movimiento dentario en humanos.
Características metodológicas de las evaluaciones de tecnologías sanitarias con evaluación multicriterio en fármacos oncológicos de alto costo en Perú 2023-2024
Authors:   , Puruguay Haro Alexandra , Vasquez Pachas Yaret Issis
Published: January 2025

El estudio presentado a continuación tiene como propósito dar una mirada la salud mental e invita a conocer cuál es la percepción de una persona que sufre depresión con respecto a su entorno laboral, abarcando en esto su trato con las personas y su desenvolvimiento con el entorno, con la finalidad de saber si perciben un clima favorable donde logre desarrollarse adecuadamente o en caso contrario, sintiéndose limitada, ignorada o estigmatizada por su condición. OBJETIVO: Identificar la relación entre la Percepción del Clima Laboral y el Nivel de Depresión en trabajadores con depresión tratada en el Centro Médico Naval. METODOLOGÍA: Se trata de un estudio analítico observacional, no probabilístico y transversal. Se evalúo a los participantes con la Escala de Depresión de Beck II y la Escala de Clima Laboral -SPC para conocer su nivel de Depresión y su Percepción del Clima Laboral, respectivamente. Los resultados se analizaron en el programa STATA donde se aplicó la Correlación de Spearman.
Liderazgo transformacional y su influencia en el clima organizacional de una entidad financiera, distrito del Cercado de Lima (Perú), 2022
Authors:   , Torres Lomas Giannina Beatriz , Curo Torres Nancy
Published: January 2025

En la investigación se tuvo como propósito principal determinar de qué manera el liderazgo transformacional repercute en el clima organizacional de una firma financiera. Para llevar a cabo el diseño metodológico de la investigación, se empleó un enfoque cuantitativo y se optó por un estudio no experimental, transeccional y correlacional-causal, con un alcance explicativo. La muestra utilizada fue censal y estuvo compuesta por 25 colaboradores, a quienes se les aplicó una encuesta mediante un cuestionario como técnica de recolección de datos. El resultado principal obtenido reveló que la variable independiente, es decir, el liderazgo transformacional, tiene una repercusión directa, positiva y significativa en el clima organizacional. Esta conclusión se basa en el análisis de regresión lineal, el cual arrojó un coeficiente de correlación de Pearson (R) de 0.791 y un coeficiente de determinación (R2) de 0.626, esto indica que el liderazgo transformacional explica el 62.6% de la variabilidad del clima organizacional en la entidad financiera estudiada. Se recomienda a la entidad financiera fortalecer el liderazgo transformacional invirtiendo en el desarrollo de habilidades de liderazgo en todos los niveles, estimulando la motivación y el compromiso de los colaboradores. La aplicación de estas medidas propiciará un entorno de trabajo más productivo y alentador, crucial para fomentar el desarrollo competitivo y materializar los objetivos de la entidad financiera.
Gestión de cobranzas y liquidez en empresas de servicios, rubro transportes, Santa Anita 2022
Authors:   , Rojas Huarcaya Gricelda
Published: January 2025

En la investigación actual con el título “Gestión de cobranzas y liquidez en empresas de servicios, rubro transporte, Santa Anita 2022”, hubo como objetivo demostrar de qué manera la gestión de cobranzas se conecta con la liquidez de las entidades de servicios, dedicadas al transporte, Santa Anita 2022., asimismo la importancia fue demostrar de qué manera la gestión de cobranzas se conecta con la liquidez de las compañías de servicios, rubro transportes, Santa Anita 2022. El método de la indagación usada fue de tipo aplicada y metodología cuantitativa, la técnica manejada ha sido encuesta y cuestionario, el instrumento realizado a una muestra de 30 empleados asociados al área de contabilidad y finanzas representantes o vinculados con las cobranzas de empresas de servicios, rubro transportes del distrito de Santa Anita. Se corroboró el objetivo general de que sí existe una relación significativa (p = 0 < 0,05) entre la gestión de cobranza y la liquidez en las entidades de servicios de transporte, Santa Anita 2022. La relación que refleja el resultado es directa, es decir, a mayor gestión de cobranza mayor liquidez, al mismo tiempo la relación es alta (= 0.693).
Relación entre el nivel de ansiedad y estrés con el perímetro abdominal en estudiantes de arquitectura en una Universidad privada, Lima – Perú, 2023-II
Authors:   , Aybar Macizo Nell Alexia
Published: January 2025

Introducción: La salud mental, el sobrepeso y la obesidad son factores que han demostrado relación a nivel fisiológico, por lo que el presente artículo tiene el objetivo de evaluar la relación entre el nivel de ansiedad y de estrés con el perímetro abdominal en estudiantes de la carrera de Arquitectura en una universidad privada, durante el periodo académico 2023-II, Lima – Perú. Métodos: Se elaboró un estudio de enfoque cuantitativo y de corte transversal, se realizó la medición antropométrica del perímetro abdominal y encuestas para medir el nivel de ansiedad y de estrés a una muestra de 227 estudiantes. Luego de ello se realizó el procesamiento de los datos a través de un análisis descriptivo para cada variable y un análisis de contingencia mediante tablas a los cuales se realizó la prueba estadística de Chi cuadrado con el programa Japs 0.17.2.1. Resultados: Revelaron que no existe una relación significativa entre el nivel de estrés / nivel de ansiedad y el perímetro abdominal de los estudiantes. Conclusión: existen otros factores que afectan significativamente los niveles de ansiedad y estrés en los estudiantes de la carrera de Arquitectura en una universidad privada.
Prácticas pedagógicas durante la educación virtual en docentes de la facultad de medicina de una universidad privada de Lima metropolitana, 2022
Authors:   , Echaiz Gamboa Isabel Flor de Maria
Published: January 2025

Esta indagación tiene como propósito determinar las prácticas pedagógicas aplicadas durante la educación virtual en docentes de la facultad de medicina. Investigación cuantitativa, básica, descriptiva y transversal, cuya muestra la conformaron 210 docentes. Para recolectar los datos se usó la encuesta y el Cuestionario de Prácticas Pedagógicas (CPP). Se evidenció que el 30% tuvo entre 41 y 50 años, el 58.6% fueron mujeres, el 71% fueron docentes médicos, el 56.2% tuvieron registro nacional de especialista (RNE), el 69% laboró como docente de pregrado en la institución entre 1 y 10 años, el 26.2% dictaba entre 21 y 30 horas al mes y el 62.4% enseñaron teoría y práctica. Se observó un alto nivel de prácticas pedagógicas aplicadas (51.4%), al igual que en sus dimensiones: enseñanza centrada en el estudiante (99%), planificación de la enseñanza (92.8%), evaluación del proceso (86.7%), enseñanza centrada en el profesor (84.8%) y uso de recursos tecnológicos (55.3%), mientras que en la dimensión relación dialogante se mostró un nivel medio (67.1%). Se concluyó que las prácticas pedagógicas aplicadas durante la educación virtual de la facultad de medicina de una universidad privada de Lima y con mayor nivel de percepción fueron las prácticas relacionadas con la enseñanza centrada en el estudiante, la planificación de la enseñanza, la evaluación del proceso, la enseñanza centrada en el profesor. Menos frecuentes fueron las prácticas pedagógicas de relación dialogante y al uso de recursos tecnológicos.
Emergency department opioid prescribing trends among provider types: An analysis of the NHAMCS, 2019-2021
Authors: French Carrson , Jackson Jace , Monahan Zach , Murray Kelly , Hartwell Micah
Published: January 2025

Background
Despite efforts to mitigate high opioid prescription frequencies, previous research showed minimal change within emergency departments (ED) in the United States, and few studies investigate prescription provider types. Thus, our primary objective was to assess opioid prescribing rates by differing healthcare team members using data from the National Hospital Ambulatory Medical Care Survey (NHAMCS).
Methods
Using the 2019–2021 NHAMCS, we calculated the overall opioid prescription rate during ED visits by provider type. Next, we estimated opioid prescription rates by provider type annually and determined differences by year using design-based X2 tests and regression models.
Results
From 2019 through 2021, 7,428 of 50,548 visits involved opioids, representing 15.62% of all ED visits. During this timeframe, 16.59% of total encounters with opioid prescriptions were among attending/consulting physicians. This was followed by physician assistants (13.91%), nurse practitioners (10.67%), and residents (7.28%). Compared to 2019, opioid prescribing rates showed no significant changes; however, resident physicians showed a significant decrease, and RNs showed a significant increase.
Discussion
From our analysis, opioid prescribing rates in the ED were highest among attending/consulting physicians, and rates among physician assistants and nurse practitioners were higher than 10%. Resident physicians had a significant decrease in opioid prescriptions, while RNs had an increase—likely due to new laws enacted during this timeframe. Removing barriers to alternative pain management for acute and long-term care may lessen rates of opioid prescriptions—including patient and provider training, physical therapists inclusion, and osteopathic manipulative therapy incorporation.
Cerebrospinal Fluid (CSF) Proteomic Signature in Preclinical and Clinical AD: Role of Adhesion Molecules.
Authors: Hajjar Ihab , Neal Reem , Yang Zhiyi , Obideen Malik , Peterson Melissa , Shah Amil
Published: January 2025

Background: Although Amyloid-beta and Tau are the hallmarks of Alzheimer’s Disease (AD), other protein pathways such as endothelial dysfunction may be involved and may precede cognitive symptoms. Our objective was to characterize the cerebrospinal fluid (CSF) proteomic profiles focusing on cardiometabolic-related protein pathways in individuals on the AD spectrum.
Methods: We performed CSF and plasma-targeted proteomics (276 proteins) from 354 participants of the Brain Stress Hypertension and Aging Program (BSHARP), of which 8% had preclinical AD, and 24% had MCI due to AD. We instituted a bioinformatic pipeline to generate data-driven protein modules, used “Hub” and "Critical” proteins within each module to describe protein signatures for each AD stage and then assessed their associations with clinical and biological AD traits. Finally, we completed pathway enrichment analysis to get insight into pathways that might be implicated in AD pathogenesis.
Results: The 276 measured proteins clustered into five modules that were associated with CSF Amyloid-β42, Tau, and pTau. (all p-value <0.05). A CSF protein AD signature was characterized by elevated levels of CSF Hepatocyte Growth Factor (HGF), Intercellular and Vascular Cell Adhesion Molecule 1 (ICAM-1, VCAM-1), Neuropilin 1 and 2 (NRP-1, NRP-2), Scavenger Receptor Class B Member 2(SCARB2), Plasminogen Activator, Urokinase (PLAU). (all <0.05) We also found a significant difference in the CSF/Plasma ratio for the proteins associated with Cognitive Status and the Tau/Aβ42 ratio (TAR) in the CSF. Pathway enrichment analysis revealed that cell adhesion and endothelial dysfuncton(all p-value <0.05) were key mechanisms involved in AD pathogenesis, especially in the preclinical stage.
Conclusion: Our results suggest a proteomic signature in the CSF of individuals with preclinical AD that is driven by adhesion molecules and might be implicated in the pathogenesis of AD. Future studies investigating these pathways may provide insights into novel AD biomarkers and therapeutic targets.
Effect of Storage Conditions on Content of 32 Pesticide Residues in Detox Waters
Authors: kıralan sezer , Gökduman Saliha
Published: January 2025

Detox water, a beverage widely consumed for weight management and health benefits, is typically prepared using fruits and vegetables such as green apple, parsley, cucumber, mint, and lemon. This study evaluated the impact of storage conditions (ambient temperature and 4°C) over 72 hours on the concentrations of 32 pesticide residues commonly found in these ingredients. Changes in pesticide residue levels and their removal rates were analyzed during storage. Results demonstrated a consistent decrease in pesticide residue concentrations with longer storage durations under both conditions. Notably, at ambient temperature, the pesticide Clofentezine exhibited the highest removal rate of 89% after 72 hours. These findings provide valuable insights into the behavior of pesticide residues in aqueous media under varying storage conditions, contributing to improved safety practices for detox water preparation and storage.
Atonik and DA-6 enriched nano Silicon fertilizer for growth and phytochemical, and mineral uptake properties enhancement in Zataria multiflora Boiss
Authors: Mostafavi Sahar , Gharneh Hoseinali Asadi , Tavallali Vahid , Rowshan Vahid
Published: January 2025

The goal of this study was to identify the growth, phytochemical composition, and nutrient uptake of Zataria multiflora using various silicon-based treatments (sodium silicate, DA-6, Atonik and green-synthesized silica-DA6, and silica-Atonik). Three concentrations (1.5, 3, and 6 mM) of each treatment were foliar-applied to thyme seedlings in a randomized complete block design. DA-6 and its nano-silicon complex (nSi-DA6) at 3 and 6 mM significantly increased shoot length and root fresh weight. DA-6 also boosted the inflorescence's fresh weight. Essential oil content increased slightly with several treatments, reaching a maximum of 3.21% with 6 mM nSi-DA6. nSi-Atonik and nSi-DA6 treatments yielded the highest levels of epicatechin and naringenin, while silica nanoparticles, nSi-Atonik, and nSi-DA6 maximized rosmarinic acid content. Silicon content increased in treated plants, but zinc and nitrogen levels remained unaffected. Enhanced growth in silica-treated plants is attributed to improved photosynthesis, transpiration, chlorophyll content, and enzyme activity. Overall, the study demonstrates that silicon-nanocomplexes treatments can positively influence Zataria multiflora growth and phytochemical production, with nSi-DA6 and nSi-Atonik showing particularly promising results.
Hydraulic Performance Optimization of Airfoil Weir-Orifice Facilities Based on Improved Hicks-Henne Shape Function and MOPSO Algorithm
Authors: Sun Bin , Liu Xiangyang , Hu Lianghan , Zhao Hangbing , LI Zhiwei , Wang Feifei
Published: January 2025

In order to better measure and control the flow rate in open channel systems, this study proposes a hydraulic performance optimization system for airfoil weir facilities. The optimization system is built around three essential modules: the modified Hicks-Henne shape function, CFD numerical simulation, and the Multi-Objective Particle Swarm Optimization (MOPSO) algorithm, which are central to airfoil shape reconstruction. In a specific example with a flow rate of 0.033 m³/s and a rotation angle of 15°, optimization concepts for head loss and submergence were applied. The results show that the optimized design reduced head loss by 9.14% and increased submergence by 5.99%. Moreover, the optimized design demonstrated excellent hydraulic performance under different angles and flow conditions. To further validate the optimization effect, the flow rate formula was derived using the π-theorem in dimensional analysis and the theory of incomplete similarity. The results indicate that the optimized airfoil weir facility’s flow rate formula is more accurate with significantly reduced errors. This study provides strong theoretical and practical support for the design and optimization of similar hydraulic structures.
EtSERPIN1 binding with chicken ANXA2 is essential for Eimeria tenella attachment and invasion process
Authors: Wang Zengbao , Li Taifeng , Jiang Yingying , Wang Xue , Li Hongmei , Zhao Xiaomin , Zhang Xiao , Zhao Ningning
Published: January 2025

Serpin protease inhibitors (SERPINs) in protozoa play crucial roles in various biological processes, including the invasion of host cells. However, the precise roles and molecular mechanisms underlying SERPIN-mediated invasion of parasite remain poorly understand. In this study, we provide evidence that surface-expressed Eimeria tenella SERPIN1 (EtSERPIN1) on sporozoites is involved in adhesion and invasion processes. To elucidate the molecular target responsible for mediating EtSERPIN1-induced invasion, we utilized GST pull-down and yeast two-hybrid verification to screen and identify host cell membrane proteins interacting with EtSERPIN1. Our findings revealed an interaction between EtSERPIN1 and a membrane protein called annexin A2 (ANXA2). Recombinant GgANXA2 was able to bind to the sporozoite surface. Furthermore, treatment with GgANXA2-specific antibody or recombinant GgANXA2 protein resulted in a dose-dependent inhibition of EtSERPIN1 binding to host cells as well as sporozoite invasion. These results suggest that EtSERPIN1 and GgANXA2 interaction plays a critical role in both adhesion and invasion processes of E. tenella sporozoites. Finally, we investigated the impact of recombinant GgANXA2 and EtSERPIN1 proteins on E. tenellainfection. Our results demonstrated that incubation with GgANXA2 protein significantly attenuated sporozoite infectivity, as evidenced by a significantly reduction in parasite burden within the chicken cecum. Immunization with recombinant EtSERPIN1 exhibited potent anti-E. tenella activity, with higher body weight gains, lower cecal lesions and oocyst output, as well as elevated levels of cecal mucosa antibodies. These findings suggest that targeting GgANXA2 through EtSERPIN1 mediates adhesion and invasion processes of E. tenella, highlighting its potential as a novel therapeutic target.
Mutational analysis of the role of conserved histidine residues of the SDHC subunit of Complex II
Authors: Yadava Nagendra
Published: January 2025

Background: SDHC protein is a critical component of the succinate-ubiquinone oxidoreductase also known as respiratory chain Complex II. Because SDHC mutations are associated with several human diseases, a detailed understanding of the role of SDHC in Complex II and overall cellular physiology is important. In this study, we have tested the functional complementation of SDHC-null cells with different variants of the SDHC proteins fused with GFP at the C-terminus. Specifically, we determined the role of four conserved histidines (H55, H71, H127, H134) in SDHC on Complex II function and mitochondrial morphology.
Methods: The SDHC-deficient Chinese hamster cell line CCL16-B9 was transfected with the plasmid constructs expressing the SDHC variants (SDHC, SDHC∆, SDHC-H55N, SDHC-H71N, SDHC-H127N, SDHC-H134N) fused with either GFP or HA-GFP at the C-terminus. Restoration of Complex II function in the transfected CCL16-B9 cells by the SDHC variants was tested by galactose selection, biochemical assays, Western blotting, and the assessment of mitochondrial morphology by fluorescence microscopy.
Results: The key findings of this study are: (1) The GFP at the C-terminus does not interfere with SDHC import, orientation, and function. (2) Wild type and mutant SDHCs can deliver GFP or HA-GFP into the IMS. (3) The third transmembrane domain is essential for SDHC function. (4) While H55, and H71are non-essential, the H127 and H134 are essential for SDHC function. The H127N and H134N mutations impair Complex II function and alter mitochondrial morphology. The most detrimental effects are due to H127N mutation. (5) SDHC can serve as a carrier for delivering the proteins of interest into IMS.
Conclusions: This study advances our understanding of the role of SDHC subunit in Complex II, mitochondrial morphology, and the overall cellular physiology.
Dynamics of ACT parasite clearance in falciparum malaria among a cohort of inhabitants in a malaria hyper-endemic focus, Ilorin Nigeria
Authors: Shittu Olalere , Obimakinde Ebenezer Tolulope , Oladosu Samuel Ololade , Abdulkodri Mubarak Damilola
Published: January 2025

Background
An appreciable reduction in parasite clearance time (PCT) following artemisinin combination therapy (ACT) is a strong prognosis for resistance, which hitherto threatens the global malaria elimination. The study exposits the accurate measurement of PCT following clinical ACT regimen among inhabitants in a malaria hyper-endemic focus, Ilorin, Nigeria.
Methods
Malaria-positive in-patients were periodically administered ACT and their parasitaemia was determined microscopically at 0, 12, 24, 36 and 72 hours. The data obtained were uploaded on the world wide antimalarial resistant network (WWARN).
Results
One hundred and twenty-one (121) valid malaria cases were evaluated using established algorithm. The following ensued, viz; lag-phase (10); median positive parasite slide (0.09) per patient; range (0.04–0.10); interquartile range (0.08–0.09); clearance rate/hour constant (K, 1/hour); distribution (N = 3, 2.48%, 0-0.05) and (N = 118, 97.52%, 0.05–0.10) respectively. The slope half-life median (range; IQR) of 7.96 (7.22–15.47; 7.74–8.42) hour. Parasite clearance was as follows; 50% (13.30; 11.86–16.78; 13.21–13.34), 90% (32.00; 30.75–47.79; 31.53–33.08), 95% (40.06; 38.23–63.26; 39.38–41.19) and 99% (58.20; 55.59–99.19; 57.55–60.74) per hour of initial value. The minus slope of the Tobit regression revealed three models, viz; best fit at 50hours (Type I); 30hours (Type II) and 20hours (Type III).
Conclusion
The dynamics with delayed parasite clearance does not entirely reflect ACT failure, but it is pertinent to monitor other early signs of resistance. Artemisinin derivatives still remain the drug of choice because of its half-life and the potentials for eliminating early ring stage.
Local Microglial Activation Induced and Labeled in the Retina in a Novel Subretinal Hemorrhage Mouse Model
Authors: Balogh Boglárka , Zille Marietta , Szarka Gergely , Péntek Loretta , Futácsi Anett , Völgyi Béla , Kovács-Öller Tamás
Published: January 2025

Subretinal hemorrhage (SRH) is caused by the accumulation of blood between the neurosensory retina and the retinal pigment epithelium or between the retinal pigment epithelium and the choroid. It often arises from age-related macular degeneration, traumas, and may occur spontaneously caused by other diseases like hypertension and diabetes. Here, we developed a novel technique by co-injection of blood and a dye-coupled tracer protein, Cholera toxin subunit B (CtB), to better localize and understand the disease and how it can cause microglial activation, inflammation, and partial vision loss. Our results show that microglia are activated in the inner retinal regions and also in the zones neighboring the blood injection. In contrast, the non-affected zone of the affected eye showed no microglial activation. Based on the results, we provide evidence of inner retinal microglial activation and the appearance of microglia and macrophages in the pigment epithelium. Using advanced imaging techniques, we were able to localize better the affected area that comprises not only the retinal area over the blood clot but the neighboring regions as well. These findings will provide the basis for novel therapeutic interventions targeting neuroinflammation in the retina after subretinal hemorrhage and other diseases affecting the eye.
First record of Ixodes keiransi (Acari: Ixodidae) ingestion by Peromyscus yucatanicus (Rodentia: Cricetidae)
Authors: Torres-Castro Marco , Suárez-Galaz Alejandro , Panti-May Jesús Alonso , Sánchez-Montes Sokani , Yam-Lozada Andrea , Yeh-Gorocica Aarón , Reyes-Novelo Enrique , Colunga-Salas Pablo , Aguilar-Tipacamú Gabriela , Rodríguez-Vivas Roger Iván
Published: January 2025

This paper aims to report the ingestion of Ixodes keiransi by Peromyscus yucatanicus. Fragments of two ticks (larvae) were collected from the intestinal content of a P. yucatanicus captured in Yucatan, Mexico. DNA was obtained from both specimens, and a fragment of 400 bp of the 16S rRNA gene was amplified and sequenced. Bioinformatics analyses were performed, and a phylogenetic tree was constructed to determine the identity of the collected ticks. Peromyscus yucatanicus is a wild rodent endemic to the Yucatan Peninsula. It carries vector-borne pathogens and the arthropods that transmit them. Its diet consists of different elements, including arthropods. However, this is the first report of this rodent's ingestion of Ixodes keiransi. This tick is a biological vector of important public health pathogens, including Rickettsia and Borrelia. This finding opens questions and hypotheses about alternative enzootic transmission mechanisms of tick-borne pathogens through tick ingestion in endemic areas.
The gain-of-function mutation of the rice auxin-signaling repressor, OsIAA13, induces rice bacterial blight resistance through activating jasmonic acid-mediating defense system
Authors: Suzuki Go , Murakami Aina , Moriyasu Yutaro , Fukuda Manatsu , Uji Yuya , Onohata Tomonori , Fujii Yumi , Inukai Yoshiaki , Gomi Kenji
Published: January 2025

Jasmonic acid (JA) is involved in the regulation of rice defense responses against Xanthomonas oryzae pv. oryzae (Xoo). JA also affects other plant hormone signaling to maximize the JA-induced defense responses in rice. In this study, we investigated the JA- and auxin-mediating defense system using a gain-of-function mutant of OsIAA13, a suppressor of auxin signaling, in rice. The expression of some auxin-responsive expansins was downregulated in the Osiaa13 mutants. The Osiaa13 mutants showed a JA-hypersensitive phenotype. The expression of some JA-responsive defense-related genes such as the lignin biosynthesis gene, OsPrx38, was upregulated in the Osiaa13 mutants. Lignin content was higher in the Osiaa13 mutants than in the wild type plants. The expression of OsPrx38 was downregulated after IAA treatment. Furthermore, some antibacterial compounds against Xoo accumulated in the Osiaa13 mutants. These results suggest that OsIAA13 has an important role in disease resistance against Xoo by regulating JA- and auxin-mediating defense system in rice.
Acceptability, feasibility, fidelity and quality implementation of the culturally adapted version of The Social Competence Promotion Program among Young Adolescents (“Mi Mejor Plan”) to prevent substance use among adolescents in Chile: a pilot randomized control study
Authors: Ramirez Saray , Ríos Natalia , Rojas-Barahona Cristian A. , Cárcamo Marcela , Sepúlveda Alejandro , Araya Ricardo , Gaete Jorge
Published: January 2025

Introduction. Substance use among adolescents is a public health problem. We culturally adapted The Social Competence Promotion Program for Young Adolescents (SCPP-YA) program to the school context in Chile (henceforth “Mi Mejor Plan or MMP”) and assessed the acceptability, feasibility, fidelity, and quality of the implementation among 6th graders. We also explored the efficacy of the program in improving individual protective factors and reducing risk factors and substance use.
Methods. Cluster randomized controlled trial conducted in Chile. The schools were randomly assigned to one of two conditions in a 1:1 ratio: 1) the "MMP" intervention group, and 2) the Control group. The program consisted of a 16-hour class-based curriculum promoting social problem-solving skills delivered by a trained facilitator. Primary outcomes were acceptability, feasibility, fidelity, and quality of the implementation using detailed reports of facilitators and from observers of the performance of the facilitators in vivo. Additionally, we explored the efficacy of the intervention on secondary outcomes: 30-day prevalence of tobacco, alcohol, and cannabis use and individual risk and protective factors promoted by MMP. We performed an intention-to-treat analysis using mixed models, taking into account the hierarchical nature of the data.
Results. 765 6th graders from 11 schools were enrolled (one school dropped out after the randomization); 608 were analyzed at baseline, and 538 were analyzed post-intervention. 52.5% were male, and the average age was 11.3 in both groups. All 16 sessions were implemented, and students’ attendance at each session ranged from 83.8% to 92.4%. The program was generally well-received, with up to 91.3% of students rating acceptability positively. Facilitators and observers reported high adherence to the contents of the program in most sessions. Protective factors, such as negative beliefs about tobacco and alcohol, increased significantly in the IG. Still, there were no significant changes in substance use, risk factors, emotion regulation, or school membership.
Conclusions. The MMP program was well accepted, and we achieved high levels of implementation and fidelity. The program improved some individual protective factors, such as negative beliefs about tobacco and alcohol, with changes in substance use among adolescents.
Trial registration. Registered on ClinicalTrials.gov, number NCT04236947.
A New Multilayer Takagi Sugeno Kang Elliptic Type- 2 Fuzzy Cerebellar-Imitated Neural Network for Nonlinear Systems Using Modified Grey-Wolf Algorithm
Authors: Huynh Tuan-Tu
Published: January 2025

This paper aims to develop a new efficient multilayer neural network structure by combining the elliptic type-2 membership function with a Takagi- Sugenno-Kang (TSK) fuzzy system and a cerebellar model articulation controller to create a new multilayer TSK elliptic type-2 fuzzy cerebellar-imitated neural network (MTET2FCNN). The MTET2FCNN can be used as the main controller in the control problem, and it is also respectively used as the main identifier and predictor for identification and prediction problems. In addition, a modified Grey-Wolf optimization algorithm is used to update the optimal learning rates of the MTET2FCNN structure. System parameter learning is performed for all rules of the proposed MTET2FCNN structure based on the gradient descent algorithm and cost function minimization. The robust design of the compensator and the system's stability are proved by Lyapunov theory. The synchronization of a chaotic 4D Rabinovich system, the identification of a time-varying system, and a Mackey-Glass time series prediction demonstrate the performance and effectiveness of the proposed system.
LEXICAL EXPRESSIVENESS AS A MEANS OF CREATING THE SOCIOCULTURAL CONTEXT OF A DETECTIVE STORY (ON THE BASIS OF THE NOVEL «THE WOMAN IN CABIN 10» BY RUTH WARE)
Authors: Yatskiv Nataliia , Huliak Tetiana
Published: January 2025

The article analyses the role of lexical means of expressiveness in shaping the sociocultural context of a detective novel, particularly in the example of the novel «The Woman in Cabin 10» by Ruth Ware. The paper explores various approaches to the study of expressiveness and context, revealing the versatility of these categories in modern literary criticism and linguistics. Expressiveness is defined as an important aspect that shapes the meaningful and emotional impact on the reader, enhancing the interactivity of the text. The research outlines the characteristics of lexical expressiveness, with special attention paid to professionalisms and barbarisms. It has been established that professionalisms emphasise the specifics of the characters’ professional environment and contribute to the creation of realistic images of the characters. Barbarisms, in turn, mark cultural differences, attract elements of the authentic linguistic environment, and enhance the intriguing atmosphere. Professionalisms and barbarisms are considered the most productive, as they add stylistic diversity to the text, emphasising the emotional state of the characters and creating a specific mood. Lexical expressions play a key role in creating a multi-layered and realistic sociocultural context in a detective novel. They not only add authenticity to the world of the work but also contribute to a deeper immersion of readers in the events and lives of the characters. As a result, the plot becomes more exciting and multifaceted, allowing readers to understand the characters’ motives and actions better and feel the atmosphere and social realities depicted in the work.
Abuso de drogas e riscos para saúde mental: promoção da saúde no contexto escolar
Authors: ALVES Pedro Lucas , HIPÓLITO José Janailson , LEITÃO Fábio Lima , OLIVEIRA Eliany Nazaré
Published: January 2025

O consumo de álcool e drogas entre adolescentes tornou-se comum, afetando muitas famílias. Isso leva ao desenvolvimento de diversas situações, incluindo dependência, e sequelas psicológicas, como depressão e ansiedade. Diante disso, este trabalho descreveu as atividades de educação em saúde promovidas por estudantes do curso de enfermagem. Trata-se de um relato de experiência das ações de extensão realizadas com estudantes de uma escola de ensino médio em Sobral-CE, durante sete encontros, em abril de 2022. O trabalho destacou a importância da comunicação sobre saúde mental e o consumo de álcool e outras drogas, ressaltando a promoção e reflexão quanto a conscientização entre os adolescentes. Os resultados demonstraram trocas significativas entre os participantes, destacando a importância de programas educativos contínuos para promover conscientização e prevenção eficazes dos jovens. Atividades dinâmicas foram essenciais para garantir a participação e o envolvimento dos adolescentes.
EMRI_MC: A GPU-based code for Bayesian inference of EMRI waveforms
Authors: Saltas Ippocratis , Oliveri Roberto
Published: January 2025

We describe a simple and efficient Python code to perform Bayesian forecasting for gravitational waves (GW) produced by Extreme-Mass-Ratio-Inspiral systems (EMRIs). The code runs on GPUs for an efficient parallelised computation of thousands of waveforms and sampling of the posterior through a Markov-Chain-Monte-Carlo (MCMC) algorithm. EMRI_MC generates EMRI waveforms based on the so-called kludge scheme, and propagates it to the observer accounting for cosmological effects in the observed waveform due to modified gravity/dark energy. The code provides a helpful resource for forecasts for interferometry missions in the milli-Hz scale, e.g the satellite-mission LISA.
Tritium concentrations in coastal waters of the Far Eastern regions of the Russian Federation in the first year of controlled water discharge from the Fukushima–1 NPP (Preliminary results)
Authors: Repin V. S. , Varfolomeeva K. V. , Biblin A. M. , Sednev K. A. , Zelentsova S. A. , Arkhangelskaya G. V.
Published: January 2025

The article presents results of a study on the tritium concentrations in seawater samples collected from September 2023 to June 2024 in coastal waters of the Far Eastern regions of the Russian Federation, including Primorsky, Kamchatka, and Khabarovsk Krais; Sakhalin and Magadan Oblasts; and the Chukotka Autonomous Okrug. The aim of the study is to determine the backgrounds values of tritium volume activity. The low-background liquid scintillation spectrometer-radiometer for alpha and beta radiation "Quantulus 1220” was used to determine the baseline tritium content in 185 seawater samples collected by specialists from the territorial bodies of Rospotrebnadzor in six subjects of the Far Eastern Federal District. The results indicate that the specific activity levels for the observation period from September 2023 to June 2024 were below 10.0 Bq/kg in samples collected from the coastal waters of the Far Eastern regions of the Russian Federation. The recorded tritium levels are significantly below the intervention level for drinking water (7600 Bq/L) as per NRB-99/2009, which applies to desalinated seawater.
Shiga toxin-producing Escherichia coli (STEC): occurrence, pathogenicity, thermal resistance.
Authors: RUDAŚ ANNA , KRUKOWSKI HENRYK
Published: January 2025

Shiga toxin-producing Escherichia coli is a zoonotic pathogen that poses a serious threat to public health. It is proven that 2.8 million acute infections occur annually, with a higher incidence in children. The infection occurs through the consumption of contaminated food of animal origin, e.g. raw or improperly heat-treated meat, milk and also through food of plant origin, e.g. vegetables, fruits and sprouts, as through the consumption of contaminated water or unpasteurized juices. Other routes of infection are human-to-human contact and human-to-animal reservoir contact. Due to the low infectious dose of STEC of only 1-100 cells, it is very important to eliminate this pathogen from food, among other things. The most commonly used method of destroying the bacteria in food processing is thermal destruction.
HOMOGENIZATION AND CRYSTALLIZATION TO IMPROVE TECHNOLOGICAL PROPERTIES OF WHOLE MILK POWDER FOR APPLICATIONS IN FOOD INDUSTRY
Authors: M. Santos Valéria , d’A. Francisquini Júlia , S. Lima Tatiana , C. Mesa Natalia , C. de Oliveira Luiz Fernando , Stephani Rodrigo , F. de Carvalho Antônio , T. Perrone Ítalo
Published: January 2025

The aim of this study was to evaluate the influence of different pressures of homogenization on whole milk powder with crystallized lactose on free fat content and particle size. Three homogenization pressure levels were applied. The powder produced at homogenization pressure of 75/5 MPa presented a smaller number of particles in the region < 1 μm (39.28%) and higher free fat content (13.78%) when compared to the powder produced at conventional homogenization pressure (15/5 MPa), which presented 59.40% particles in the region < 1 μm and 5.03% of free fat. Milk powder produced at 0/0 MPa presented the lowest number of particles in the region < 1 μm (24.60%) and the highest free fat content (19.39%). This study showed that the powder produced at a homogenization pressure of 15/5 MPa, which is commonly used in the industry, produces a crystallized milk powder with less suitable properties than the one obtained without homogenization.
Social Mapping: Sustainable Livelihood Approach Karyawangi Village, Pulosari Subdistrict, Pandeglang District
Authors: Sumintak Sumintak , Nurdin Kamaluddin , Sukirno Agus , Kurniawan Ade Fakih , Hunainah Hunainah
Published: January 2025

Social mapping is a crucial step in effective community empowerment. This research combines the Asset-Based Community Development (ABCD) framework with the Sustainable Livelihood Approach (SLA) to analyze the potential of community assets in Employeesgi Village, Pulosari District, Pandeglang Regency. The research aims to identify key assets such as natural resources, human capital, social networks, financial capital, and infrastructure to support sustainable development. The research method involved secondary data collection, questionnaires, focus group discussions (FGD) surveys, and purposive sampling techniques with 64 respondents. The research results show that the employee village has good infrastructure assets but faces human and financial capital challenges. Integrating local potential with a targeted development approach produces strategic recommendations, including skills training, financial management, developing nature-based tourism, and improving waste management and transportation access. In conclusion, by optimally utilizing local assets, community empowerment can increase economic resilience and social cohesion while encouraging sustainable development.
Rendimiento en aserrío de trozas de raleo de Pinus patula de 22 años en Cajamarca, Perú
Authors: Montenegro Muro Rolando , Meléndez Cárdenas Miguel
Published: January 2025

El presente estudio analiza el rendimiento en aserrío de trozas de raleo de Pinus patula de 22 años y evalúa la mejor forma de estimar el volumen de madera aserrada a obtener, en Porcón, Perú. Se halló que las trozas poseen un diámetro promedio de 25.1 cm y que el rendimiento promedio es de 43.2 % considerado bajo. Se encontró que la calidad del 81.1 % de las trozas aserradas corresponde a primera y solo 4.7 % son de tercera. Asimismo, el 54.3 % de las tablas producidas pertenece a la denominación Especial y el 29.3 % a la denominación comercial. Se demuestra que no existe relación entre el rendimiento y el diámetro de la troza y que la mejor estimación del volumen de madera aserrada se obtiene a partir del diámetro menor de la troza y no a través del Coeficiente de Conversión.