bluuebunny/crossref_metadata_2025 huggingface dataset:
Hematological- and Immunological-Related Biomarkers to Characterize Patients with COVID-19 from Other Viral Respiratory Diseases
Authors: Suárez-Del-Villar-Carrero Rafael , Martinez-Urbistondo Diego , Cuevas-Sierra Amanda , Ibañez-Sustacha Iciar , Candela-Fernandez Alberto , Dominguez-Calvo Andrea , Ramos-Lopez Omar , Vargas Juan Antonio , Reglero Guillermo , Villares-Fernandez Paula , Martinez Jose Alfredo
Published: June 2022

COVID-19 has overloaded health system worldwide; thus, it demanded a triage method for an efficient and early discrimination of patients with COVID-19. The objective of this research was to perform a model based on commonly requested hematological variables for an early featuring of patients with COVID-19 form other viral pneumonia. This investigation enrolled 951 patients (mean of age 68 and 56% of male) who underwent a PCR test for respiratory viruses between January 2019 and January 2020, and those who underwent a PCR test for detection of SARS-CoV-2 between February 2020 and October 2020. A comparative analysis of the population according to PCR tests and logistic regression model was performed. A total of 10 variables were found for the characterization of COVID-19: age, sex, anemia, immunosuppression, C-reactive protein, chronic obstructive pulmonary disease, cardiorespiratory disease, metastasis, leukocytes and monocytes. The ROC curve revealed a sensitivity and specificity of 75%. A deep analysis showed low levels of leukocytes in COVID-19-positive patients, which could be used as a primary outcome of COVID-19 detection. In conclusion, this investigation found that commonly requested laboratory variables are able to help physicians to distinguish COVID-19 and perform a quick stratification of patients into different prognostic categories.
Particle-Reinforced Ceramic Matrix Composites—Selected Examples
Authors: Konopka Katarzyna
Published: June 2022

This paper presents some examples of ceramic matrix composites (CMCs) reinforced with metal or intermetallic phases fabricated by powder consolidation without a liquid phase (melted metal). Composites with a complex structure, which are an advanced group of CMCs called hybrid composites, were described in contrast to conventional composites with a ceramic matrix. In advanced CMCs, their complex structures make it possible to achieve the synergistic effect of the micro- and nanoparticles of the metallic, intermetallic, and ceramic phases on the composite properties, which is not possible in conventional materials. Various combinations of substrates in the form of powder as more than one metal and ceramics with different powder sizes that are used to form hybrid composites were analyzed. The types of CMC microstructures, together with their geometrical schemas and some examples of real ceramic matrix composites, were described. The schemas of composite microstructures showed the possible location of the ceramic, metallic, or intermetallic phases in composites. A new concept of an advanced ceramic–intermetallic composite fabricated by the consolidation of pre-composite powder mixed with ceramic powder was also presented. This concept is based on the selection of substrates, two metals in the form of powder, which will form a new compound, intermetallic material, during processing. Metal powders were milled with ceramic powders to obtain a pre-composite powder consisting of intermetallic material and ceramics. In the next step, the consolidation of pre-composite powder with ceramic powder allows the creation of composites with complex microstructures. Selected examples of real particle-reinforced conventional and hybrid microstructures based on our own investigations were presented. In addition to microstructures, the properties and possible applications of CMCs were analyzed.
No-Reference Quality Assessment of Authentically Distorted Images Based on Local and Global Features
Authors: Varga Domonkos
Published: June 2022

With the development of digital imaging techniques, image quality assessment methods are receiving more attention in the literature. Since distortion-free versions of camera images in many practical, everyday applications are not available, the need for effective no-reference image quality assessment algorithms is growing. Therefore, this paper introduces a novel no-reference image quality assessment algorithm for the objective evaluation of authentically distorted images. Specifically, we apply a broad spectrum of local and global feature vectors to characterize the variety of authentic distortions. Among the employed local features, the statistics of popular local feature descriptors, such as SURF, FAST, BRISK, or KAZE, are proposed for NR-IQA; other features are also introduced to boost the performances of local features. The proposed method was compared to 12 other state-of-the-art algorithms on popular and accepted benchmark datasets containing RGB images with authentic distortions (CLIVE, KonIQ-10k, and SPAQ). The introduced algorithm significantly outperforms the state-of-the-art in terms of correlation with human perceptual quality ratings.
High-Capacity Reversible Data Hiding in Encrypted Images with Flexible Restoration
Authors: Arai Eichi , Imaizumi Shoko
Published: June 2022

In this paper, we propose a novel reversible data hiding in encrypted images (RDH-EI) method that achieves the highest hiding capacity in the RDH-EI research field and full flexibility in the processing order without restrictions. In the previous work in this field, there exist two representative methods; one provides flexible processing with a high hiding capacity of 2.17 bpp, and the other achieves the highest hiding capacity of 2.46 bpp by using the BOWS-2 dataset. The latter method has critical restrictions on the processing order. We focus on the advantage of the former method and introduce two efficient algorithms for maximizing the hiding capacity. With these algorithms, the proposed method can predict each pixel value with higher accuracy and refine the embedding algorithm. Consequently, the hiding capacity is effectively enhanced to 2.50 bpp using the BOWS-2 dataset, and a series of processes can be freely conducted without considering any restrictions on the order between data hiding and encryption. In the same way, there are no restrictions on the processing order in the restoration process. Thus, the proposed method provides flexibility in the privileges requested by users. Experimental results show the effectiveness of the proposed method in terms of hiding capacity and marked-image quality.
±0.3V Bulk-Driven Fully Differential Buffer with High Figures of Merit
Authors: Gangineni Manaswini , Ramirez-Angulo Jaime , Vázquez-Leal Héctor , Huerta-Chua Jesús , Lopez-Martin Antonio J. , Carvajal Ramon Gonzalez
Published: June 2022

A high performance bulk-driven rail-to-rail fully differential buffer operating from ±0.3V supplies in 180 nm CMOS technology is reported. It has a differential–difference input stage and common mode feedback circuits implemented with no-tail, high CMRR bulk-driven pseudo-differential cells. It operates in subthreshold, has infinite input impedance, low output impedance (1.4 kΩ), 86.77 dB DC open-loop gain, 172.91 kHz bandwidth and 0.684 μW static power dissipation with a 50-pF load capacitance. The buffer has power efficient class AB operation, a small signal figure of merit FOMSS = 12.69 MHzpFμW−1, a large signal figure of merit FOMLS = 34.89 (V/μs) pFμW−1, CMRR = 102 dB, PSRR+ = 109 dB, PSRR− = 100 dB, 1.1 μV/√Hz input noise spectral density, 0.3 mVrms input noise and 3.5 mV input DC offset voltage.
Analysis of the Mechanical Properties of the Reconstituted Hydrate-Bearing Clayey-Silt Samples from the South China Sea
Authors: Dong Lin , Liao Hualin , Li Yanlong , Meng Qingguo , Hu Gaowei , Wang Jintang , Wu Nengyou
Published: June 2022

Mechanical properties of hydrate-bearing sediments (HBS) are crucial for evaluating drilling- and production-induced geo-hazards. However, investigations on mechanical behaviors of clayey-silt samples containing hydrate are insufficient due to low efficiency in preparing reconstituted hydrate-bearing samples. Herein, we carried out a series of triaxial shear tests to analyze the deformation behaviors of reconstituted clayey-silt samples containing tetrahydrofuran (THF) hydrate. The sediments were taken from the Shenhu Area, northern South China Sea. The failure mechanisms during shearing are discussed based on micro-to-macro analyses. The results imply that the stress-strain curves show obvious strain-hardening under triaxial shearing, which can be divided into elastic deformation stage, transitional stage, and plastic deformation stage. Besides, the results reveal that cohesion strengthens from 0.09 MPa to 1.28 MPa when hydrate saturation increases from 15% to 60%. Moreover, calculation models are proposed to evaluate failure strengths and Young’s modulus. Establishing empirical formula based on experimental data can quickly determine the strength parameters with knowing the hydrate saturation and stress state of clayey-silt sediments containing hydrate. It is urgent in field operations and numerical simulation to use reliable empirical models.
The Thermal Effect of Submarine Mud Volcano Fluid and Its Influence on the Occurrence of Gas Hydrates
Authors: Wan Zhifeng , Luo Junsheng , Yang Xiaolu , Zhang Wei , Liang Jinqiang , Zuo Lihua , Sun Yuefeng
Published: June 2022

Mud volcanoes and other fluid seepage pathways usually transport sufficient gas for the formation of gas reservoirs and are beneficial to the accumulation of gas hydrate. On the other hand, the fluid thermal effects of mud volcanoes can constrain the occurrence of gas hydrates. Current field measurements indicate that fluid thermal anomalies impact the distribution of gas hydrates associated with mud volcanoes. However, due to the lack of quantitative analysis of the mud volcano fluid flow and thermal evolution, it is difficult to effectively reveal the occurrence of gas hydrates in mud volcano development areas and estimate their resource potential. This study took the Håkon Mosby Mud Volcano (HMMV) in the southwestern Barents Sea as the research object and comprehensively used seismic, well logging, drilling and heat flow survey data, combining the principles and methods of fluid dynamics and thermodynamics to study the fluid flow and heat transfer of a mud volcanic pathway. The space framework of the mud volcanic fluid temperature field thermal structure was established, the influence of the HMMV fluid thermal effect on gas hydrate occurrence was analyzed and the distribution and resource potential of gas hydrates in mud volcano development areas were revealed from the perspective of thermodynamics. This study provides a thermodynamic theoretical basis for gas hydrate accumulation research, exploration and exploitation under a fluid seepage tectonic environment.
Object Recognition and Tracking in Moving Videos for Maritime Autonomous Surface Ships
Authors: Park Hyunjin , Ham Seung-Ho , Kim Taekyeong , An Donghyeok
Published: June 2022

In autonomous driving technologies, a camera is necessary for establishing a path and detecting an object. Object recognition based on images from several cameras is required to detect impediments in autonomous ships. Furthermore, in order to avoid ship collisions, it is important to follow the movements of recognized ships. In this paper, we use the Singapore Maritime Dataset (SMD) and crawling image for model training. Then, we present four YOLO-based object recognition models and evaluate their performance in the maritime environment. Then, we propose a tracking algorithm to track the identified objects. Specially, in evaluation with high-motion video, the proposed tracking algorithm outperforms deep simple online and real-time tracking (DeepSORT) in terms of object tracking accuracy.
Methylmercury, Trace Metals, Organotins and Their Effects on the Qatari Mangrove Shrimp, Palaemon khori
Authors: Hassan Hassan , Benvenuto Chiara , Al-Maslamani Ibrahim , Chatting Mark , Mondal Debapriya , Leitão Alexandra
Published: June 2022

The Qatari mangroves of Al-Khor are being increasingly exposed to a wide variety of anthropogenic pollutants due to land reclamation and urban expansion. In this study, we evaluated the lethal and genotoxic effects of methylmercury, trace metals, and organotins, assessing mortality and aneuploidy levels (abnormal number of chromosomes) in the endemic shrimp Palaemon khori under laboratory conditions. In the experimental design, two different concentrations were used for each family of contaminant (single or combined): an environmental concentration equivalent to the maximum value reported in the environment and a value ten times higher, for a period of eight weeks. Survival decreased significantly when pollutants were administrated in combination, even at environmental concentrations (as shown by Cox proportional hazards ratios): similar levels of mortality would be reached by individual type of pollutants only at ten times the environmental concentration. This critical result, under controlled lab conditions, highlights the importance of monitoring mixtures of contaminant types over single ones in the marine environment. Aneuploidy was reported in all treatments and control ranging from 5% to 19% at week four and from 7% to 21% at week eight. All treatments presented significantly higher aneuploidy levels when compared to the control. However, no significant difference was observed between the two time periods, even though 30% of the treatments could not be assessed at week eight, as not enough animals were still alive. In conclusion, the use of endemic species should be considered a valuable tool to determine local perturbations, representing a regional bioindicator of multiple environmental stressors from the initial stages of contamination.
Microalgae-Based PUFAs for Food and Feed: Current Applications, Future Possibilities, and Constraints
Authors: Santin Anna , Balzano Sergio , Russo Monia Teresa , Palma Esposito Fortunato , Ferrante Maria Immacolata , Blasio Martina , Cavalletti Elena , Sardo Angela
Published: June 2022

Microalgae are currently considered an attractive source of highly valuable compounds for human and animal consumption, including polyunsaturated fatty acids (PUFAs). Several microalgae-derived compounds, such as ω-3 fatty acids, pigments, and whole dried biomasses are available on the market and are mainly produced by culturing microalgae in open ponds, which can be achieved with low setup and maintenance costs with respect to enclosed systems. However, open tanks are more susceptible to bacterial and other environmental contamination, do not guarantee a high reproducibility of algal biochemical profiles and productivities, and constrain massive cultivation to a limited number of species. Genetic engineering techniques have substantially improved over the last decade, and several model microalgae have been successfully modified to promote the accumulation of specific value-added compounds. However, transgenic strains should be cultured in closed photobioreactors (PBRs) to minimize risks of contamination of aquatic environments with allochthonous species; in addition, faster growth rates and higher yields of compounds of interest can be achieved in PBRs compared to open ponds. In this review, we present information collected about the major microalgae-derived commodities (with a special focus on PUFAs) produced at industrial scale, as well genetically-engineered microalgae to increase PUFA production. We also critically analyzed the main bottlenecks that make large-scale production of algal commodities difficult, as well as possible solutions to overcome the main problems and render the processes economically and environmentally safe.
Serological Response and Relationship with Gender-Sensitive Variables among Healthcare Workers after SARS-CoV-2 Vaccination
Authors: Cangemi Roberto , Di Franco Manuela , Angeloni Antonio , Zicari Alessandra , Cardinale Vincenzo , Visentini Marcella , Antonelli Guido , Napoli Anna , Anastasi Emanuela , Romiti Giulio Francesco , d’Alba Fabrizio , Alvaro Domenico , Polimeni Antonella , Basili Stefania ,   SAPIENZAVAX Collaborators
Published: June 2022

Vaccine-induced immunity is a key strategy in the long-term control of the COVID-19 pandemic. The aim of our study was to explore the relationship between mRNA vaccine-induced antibodies and gender-sensitive variables among healthcare workers. Two thousand-sixty-five volunteers who received the BNT162b2 vaccine were enrolled in the study and followed up. Demographic, clinical, and social variables (educational level, marital status, occupation, childcare) were evaluated through a self-administered questionnaire. Anti-Spike (S) IgG were measured at 1 month (T1) and at 5 months (T2) after the second vaccine dose. At T1, median anti-S IgG values were 693 [394–>800] AU/mL (1 AU = 2.6 BAU). Values > 800 AU/mL (2080 BAU/mL) were directly associated with a previous COVID-19 (p < 0.001) infection and inversely with age (p < 0.001), smoking habit (p < 0.001), and autoimmune diseases (p < 0.001). At T2, a significant decreasing in anti-S IgG values was observed (187 [81–262] AU/mL), with a median decrease of 72 [60–82]%. On multivariate data analysis, a reduction of more than 82% was directly associated with male sex (p < 0.021), age (p < 0.001), smoking (p = 0.038), hypertension (p = 0.042), and, inversely, with previous COVID-19 infection (p < 0.001) and being “cohabiting” (p = 0.005). Our findings suggest that demographic, clinical, and social variables play a role in anti-S IgG values decreasing in long-term follow up and should be considered to find personalized vaccine schedules.
Physiologic Range of Myocardial Mechano-Energetic Efficiency among Healthy Subjects: Impact of Gender and Age
Authors: Ferrara Francesco , Capone Valentina , Cademartiri Filippo , Vriz Olga , Cocchia Rosangela , Ranieri Brigida , Franzese Monica , Castaldo Rossana , D’Andrea Antonello , Citro Rodolfo , Chianese Salvatore , Annunziata Roberto , Marullo Flavio , Siniscalchi Mario , Conte Marianna , Sepe Chiara , Maramaldi Renato , Rega Salvatore , Russo Giuseppe , Majolo Massimo , Raiola Eliana , Salzano Andrea , Mauro Ciro , Trimarco Bruno , Izzo Raffaele , Bossone Eduardo
Published: June 2022

Background: Myocardial mechano-energetic efficiency (MEE) is the capability of the left ventricle (LV) to convert the chemical energy obtained from the cardiac oxidative metabolism into mechanical work. The aim of present study was to establish normal non-invasive MEE and MEEi reference values. Methods: In total, 1168 healthy subjects underwent physical examinations, clinical assessment, and standardized transthoracic echocardiographic (TTE) examination. MEE was obtained by TTE as the ratio between stroke volume (SV) and heart rate (HR): MEE = SV/HR [HR expressed in seconds (HR/60)]. Because MEE is highly related to left ventricular mass (LVM), MEE was then divided by LVM with the purpose of obtaining an estimate of energetic expenditure per unit of myocardial mass (i.e., indexed MEE, MEEi, mL/s/g). Results: The mean values of MEE and MEEi in the overall population were 61.09 ± 18.19 mL/s; 0.45 ± 0.14, respectively. In a multivariable analysis, gender, body surface area (BSA), diastolic blood pressure, left atrial volume indexed to BSA, E/e’ and tricuspid annular plane systolic excursion (TAPSE) were the independent variables associated with MEE, while age, gender, BSA and TAPSE were the independent variables associated with MEEi. Conclusions: The knowledge of age- and gender-based MEE and MEEi normal values may improve the global assessment of LV cardiac mechanics and serve as a reference to identify phenotypes at high risk of cardiovascular events.
Rapid Personalised Virtual Planning and On-Demand Surgery for Acute Spinal Trauma Using 3D-Printing, Biomodelling and Patient-Specific Implant Manufacture
Authors: Mobbs Ralph Jasper , Parr William C. H. , Huang Christopher , Amin Tajrian
Published: June 2022

Three-dimensional printing is a rapidly growing field, with extensive application to orthopaedics and spinal surgery. Three-dimensional-printed (3DP) patient-specific implants (PSIs) offer multiple potential benefits over generic alternatives, with their use increasingly being described in the spinal literature. This report details a unique, emergency case of a traumatic spinal injury in a 31-year-old male, acquired rurally and treated with a 3DP PSI in a tertiary unit. With increasing design automation and process improvements, rapid, on-demand virtual surgical planning (VSP) and 3DP PSIs may present the future of orthopaedics and trauma care, enabling faster, safer, and more cost-effective patient-specific procedures.
Three-Dimensional Postoperative Results Prediction for Orthognathic Surgery through Deep Learning-Based Alignment Network
Authors: Jeong Seung Hyun , Woo Min Woo , Shin Dong Sun , Yeom Han Gyeol , Lim Hun Jun , Kim Bong Chul , Yun Jong Pil
Published: June 2022

To date, for the diagnosis of dentofacial dysmorphosis, we have relied almost entirely on reference points, planes, and angles. This is time consuming, and it is also greatly influenced by the skill level of the practitioner. To solve this problem, we wanted to know if deep neural networks could predict postoperative results of orthognathic surgery without relying on reference points, planes, and angles. We use three-dimensional point cloud data of the skull of 269 patients. The proposed method has two main stages for prediction. In step 1, the skull is divided into six parts through the segmentation network. In step 2, three-dimensional transformation parameters are predicted through the alignment network. The ground truth values of transformation parameters are calculated through the iterative closest points (ICP), which align the preoperative part of skull to the corresponding postoperative part of skull. We compare pointnet, pointnet++ and pointconv for the feature extractor of the alignment network. Moreover, we design a new loss function, which considers the distance error of transformed points for a better accuracy. The accuracy, mean intersection over union (mIoU), and dice coefficient (DC) of the first segmentation network, which divides the upper and lower part of skull, are 0.9998, 0.9994, and 0.9998, respectively. For the second segmentation network, which divides the lower part of skull into 5 parts, they were 0.9949, 0.9900, 0.9949, respectively. The mean absolute error of transverse, anterior–posterior, and vertical distance of part 2 (maxilla) are 0.765 mm, 1.455 mm, and 1.392 mm, respectively. For part 3 (mandible), they were 1.069 mm, 1.831 mm, and 1.375 mm, respectively, and for part 4 (chin), they were 1.913 mm, 2.340 mm, and 1.257 mm, respectively. From this study, postoperative results can now be easily predicted by simply entering the point cloud data of computed tomography.
Biologic Therapies in Pediatric Asthma
Authors: Perikleous Evanthia P. , Steiropoulos Paschalis , Nena Evangelia , Paraskakis Emmanouil
Published: June 2022

Undeniably, childhood asthma is a multifactorial and heterogeneous chronic condition widespread in children. Its management, especially of the severe form refractory to standard therapy remains challenging. Over the past decades, the development of biologic agents and their subsequent approval has provided an advanced and very promising treatment alternative, eventually directing toward a successful precision medicine approach. The application of currently approved add-on treatments for severe asthma in children, namely omalizumab, mepolizumab, benralizumab, dupilumab, and tezepelumab have been shown to be effective in terms of asthma control and exacerbation rate. However, to date, information is still lacking regarding its long-term use. As a result, data are frequently extrapolated from adult studies. Thus, the selection of the appropriate biologic agent, the potential predictors of good asthma response, and the long-term outcome in the pediatric population are still to be further investigated. The aim of the present study was to provide an overview of the current status of the latest evidence about all licensed monoclonal antibodies (mAbs) that have emerged and been applied to the field of asthma management. The innovative future targets are also briefly discussed.
Venous Segmental Flow Changes after Superficial Venous Intervention Demonstrating by Quantitative Phase-Contrast Magnetic Resonance Analysis: Preliminary Data from a Longitudinal Cohort Study
Authors: Chen Chien-Wei , Tseng Yuan-Hsi , Kao Chih-Chen , Ngo Yeh Giin , Lee Chung-Yuan , Yang Teng-Yao , Lin Yu-Hui , Huang Yao-Kuang
Published: June 2022

The effects of superficial venous intervention on hemodynamics can be quantified using two-dimensional phase-contrast magnetic resonance imaging (2D PC-MRI). Twelve patients received pre- and postintervention 2D PC-MRI analysis using quantitative hemodynamic parameters. Fifteen healthy volunteers served as controls. The 2D PC-MRI results of the target limbs (limbs scheduled for intervention for venous reflux) differed from those of the controls in terms of stroke volume (SV), forward flow volume (FFV), absolute stroke volume (ASV), and mean flux (MF) in all venous segments. The velocity time integral (VTI) and mean velocity (MV) of the popliteal vein (PV) segments were similar between the target limbs and controls preoperatively. After intervention, the target limbs exhibited an increase in VTI and MV in the femoral vein (FV) and PV segments. We compared the target and nontreated limbs of the individual patients preoperatively and postoperatively to minimalize individual bias. All QFlow parameter ratios in the FV segment increased after venous intervention (VTI, p = 0.025; MV, p = 0.024). In the PV segment, FFV and ASV increased significantly (p = 0.035 and 0.024, respectively). After interventions, the volume (FFV and ASV) of the PV segment and the efficiency (VTI and MV) of the FV segment significantly increased.
Co-Designing an Integrated Care Network with People Living with Parkinson’s Disease: A Heterogeneous Social Network of People, Resources and Technologies
Authors: Gauthier-Beaupré Amélie , Poitras Emely , Grosjean Sylvie , Mestre Tiago A. ,   on behalf of the iCARE-PD Consortium
Published: June 2022

As part of the iCARE-PD project, a multinational and multidisciplinary research endeavour to address complex care in Parkinson’s disease, a Canadian case study focused on gaining a better understanding of people living with Parkinson’s disease (PwP) experiences with health and medical services, particularly their vision for a sustainable, tailored and integrated care delivery network. The multifaceted nature of the condition means that PwP must continuously adapt and adjust to every aspect of their lives, and progressively rely on support from care partners (CP) and various health care professionals (HCP). To envision the integrated care delivery network from the perspective of PwP, the study consisted of designing scenarios for an integrated care delivery network with patients, their CP and their HCP, as well as identifying key requirements for designing an integrated care delivery network. The results demonstrate that numerous networks interact, representing specific inscriptions, actors and mediators who meet at specific crossing points. This resulted in the creation of a roadmap and toolkit that takes into consideration the unique challenges faced by PwP, and the necessity for an integrated care delivery network that can be personalized and malleable so as to adapt to evolving and changing needs over time.
Translated Mutant DSPP mRNA Expression Level Impacts the Severity of Dentin Defects
Authors: Kim Youn Jung , Lee Yejin , Zhang Hong , Seymen Figen , Koruyucu Mine , Bayrak Sule , Tuloglu Nuray , Simmer James P. , Hu Jan C.-C. , Kim Jung-Wook
Published: June 2022

Hereditary dentin defects are conventionally classified into three types of dentinogenesis imperfecta (DGI) and two types of dentin dysplasia (DD). Mutations in the dentin sialophosphoprotein (DSPP) gene have been identified to cause DGI type II and III and DD type II; therefore, these are not three different conditions, but rather allelic disorders. In this study, we recruited three families with varying clinical phenotypes from DGI-III to DD-II and performed mutational analysis by candidate gene analysis or whole-exome sequencing. Three novel mutations including a silent mutation (NM_014208.3: c.52-2del, c.135+1G>C, and c.135G>A; p.(Gln45=)) were identified, all of which affected pre-mRNA splicing. Comparison of the splicing assay results revealed that the expression level of the DSPP exon 3 deletion transcript correlated with the severity of the dentin defects. This study did not only expand the mutational spectrum of DSPP gene, but also advanced our understanding of the molecular pathogenesis impacting the severity of hereditary dentin defects.
IL-10 Induced by mTNF Crosslinking-Mediated Reverse Signaling in a Whole Blood Assay Is Predictive of Response to TNFi Therapy in Rheumatoid Arthritis
Authors: Krasselt Marco , Gruz Natalya , Pierer Matthias , Baerwald Christoph , Wagner Ulf
Published: June 2022

(1) Background: To date, the response of patients with rheumatoid arthritis (RA) to the various biologic DMARD available cannot be predicted due to a lack of reliable biomarkers. Based on our preliminary work on tmTNF reverse signaling, we developed a whole-blood assay measuring tmTNF crosslinking-induced IL-10 production to predict the response to TNF inhibitor (TNFi) therapy. (2) Methods: This prospective study included patients with active RA. Depending on the clinical judgment of the attending rheumatologist, either therapy with a TNF or JAK inhibitor was initiated. Clinical parameters and blood samples were obtained at baseline and after 8 weeks of therapy. The blood samples were collected using a newly developed whole-blood assay based on the principle of tmTNF reverse signalling. Subsequently, IL-10 was measured via enzyme-linked immunosorbent assay (ELISA) technique. (3) Results: 63 patients with RA were enrolled. In fifteen patients, TNFi therapy was initiated, while eight patients started a JAKi treatment. The cross-sectional analysis of all patients showed a positive correlation between tmTNF crosslinking-induced IL-10 and parameters of disease activity (CRP [r = 0.4091, p = 0.0009], DAS28 [r = 0.3303, p = 0.0082]) at baseline. In the TNFi treatment study, IL-10 was found to be significantly higher in EULAR responders than in non-responders (p = 0.0033). After initiation of JAKi treatment, in contrast, IL-10 induction was not linked to response. Longitudinal analysis of the TNFi-treated patients revealed IL-10 to decrease in responders (p = 0.04), but not in non-responders after 8 weeks of therapy. Of importance, the IL-10 production at baseline correlated inversely with TNFi response determined by ΔDAS28 in patients with TNFi treatment (r = −0.5299, p = 0.0422) while no such link was observed under JAKi therapy (p = 0.22). Receiver operation characteristics (ROC) analysis demonstrated a high performance of tmTNF/crosslinking-induced IL-10 in predicting a TNFi therapy response according to the EULAR criteria (AUC = 0.9286, 95% Confidence interval 0.7825–1.000, p = 0.0055). (4) Conclusions: In this pilot investigation, we demonstrated the feasibility of a whole-blood assay measuring tmTNF-induced IL-10 to predict clinical response to TNF inhibitor treatment. This approach might support rheumatologists in their decision for an individually tailored RA therapy.
Gastric Cancer Angiogenesis Assessment by Dynamic Contrast Harmonic Imaging Endoscopic Ultrasound (CHI-EUS) and Immunohistochemical Analysis—A Feasibility Study
Authors: Sacerdoțianu Victor Mihai , Ungureanu Bogdan Silviu , Iordache Sevastiţa , Cazacu Sergiu Marian , Pirici Daniel , Liliac Ilona Mihaela , Burtea Daniela Elena , Șurlin Valeriu , Stroescu Cezar , Gheonea Dan Ionuț , Săftoiu Adrian
Published: June 2022

Tumor vascular perfusion pattern in gastric cancer (GC) may be an important prognostic factor with therapeutic implications. Non-invasive methods such as dynamic contrast harmonic imaging endoscopic ultrasound (CHI-EUS) may provide details about tumor perfusion and could also lay out another perspective for angiogenesis assessment. Methods: We included 34 patients with GC, adenocarcinoma, with CHI-EUS examinations that were performed before any treatment decision. We analyzed eighty video sequences with a dedicated software for quantitative analysis of the vascular patterns of specific regions of interest (ROI). As a result, time-intensity curve (TIC) along with other derived parameters were automatically generated: peak enhancement (PE), rise time (RT), time to peak (TTP), wash-in perfusion index (WiPI), ROI area, and others. We performed CD105 and CD31 immunostaining to calculate the vascular diameter (vd) and the microvascular density (MVD), and the results were compared with CHI-EUS parameters. Results: High statistical correlations (p < 0.05) were observed between TIC analysis parameters MVD and vd CD31. Strong correlations were also found between tumor grade and 7 CHI-EUS parameters, p < 0.005. Conclusions: GC angiogenesis assessment by CHI-EUS is feasible and may be considered for future studies based on TIC analysis.
How Has the COVID-19 Pandemic Affected the Different Branches of the Agri-Food Industry in Extremadura (Spain)?
Authors: Sama-Berrocal Celia , Martínez-Azúa Beatriz Corchuelo
Published: June 2022

The COVID-19 pandemic has had a significant impact on the world economy since 2020. This study analyzed the impact of the pandemic on innovative agri-food companies from different branches of agro-industrial activity located in Extremadura (Spain). The main aim of this study was to determine which activities have been most affected. Differences between actions and changes made depending on the nature of the product, process, or services were also evaluated. The information was obtained from an online questionnaire in which the research questions were posed (what consequences, actions, or changes has the pandemic had on the development of firms’ activities?). Data were analyzed descriptively, and a statistical study was conducted on the existence or absence of independence between effects and actions based on the branches of activity of agri-food industries. The main results showed that companies’ financial (decrease in turnover and reduction/displacement of product demand) and operational functioning (difficulty in marketing activities and standstill/decline in the fiscal year) has mainly been affected. In response, innovative agro-industries have acted regarding their processes (increased ICT use and new marketing strategies) and procedures (implementation of stricter hygienic-sanitary protocols and reorganization of activities and personnel) to deal with the negative effects on their activities. In general, all agro-industrial branches have incorporated changes in their products and services, mainly by providing new and better customer benefits, and improving product formats and forms of payment to suppliers. These findings provide information for the regional public administration in the development of initiatives that mitigate the negative effects of the pandemic and favor the implementation of actions that help the adaptation of agro-industrial activities. Agricultural policies should incorporate specialized measures to ensure the global sustainability of the food and agriculture system and the supply and production.
Exploring Changes in Land Use and Landscape Ecological Risk in Key Regions of the Belt and Road Initiative Countries
Authors: Zhang Xuebin , Yao Litang , Luo Jun , Liang Wenjuan
Published: June 2022

The Belt and Road Initiative (BRI) has revealed that it is necessary to strengthen research on land use and land cover change (LUCC) and ecological risk in key regions of countries around the world. In this study, the spatiotemporal characteristics of LUCC in the five capitals of Central Asian countries within the BRI were analyzed. Based on the grid scale, a landscape pattern index was introduced to quantitatively evaluate the landscape ecological risk levels of the five capitals. The results showed the following: first, the components of land use types in the five capitals have different structural characteristics, which are mainly grassland, unused land, and cultivated land. The landscape types that changed significantly were water and unused land, while the construction land area showed a trend of continuous increase. Second, different capitals have different land-use transfer patterns. Akmola State is mainly converted from cultivated land to grassland; Chuy State is mainly converted from forest land to grassland; Dushanbe and Tashkent City are mainly converted from grassland to forestland; and Ahal State is mainly converted from grassland to unused land. Third, the overall landscape ecological risks of the five capitals were low. Akmola State had the largest proportion of lowest ecological risk areas, whereas Chuy State and Dushanbe City had an increasing trend of highest ecological risk areas. The level of ecological risk in Tashkent remained stable during the study period, and the highest ecological risk areas in Ahal State decreased to 49,227.86 km2. This study has enriched the research results of land use change and landscape ecological risk assessment of countries within the BRI and can provide a research reference for these countries and regions to achieve ecological sustainable development and strengthen ecosystem management.
A Dynamic Performance and Differentiation Management Policy for Urban Construction Land Use Change in Gansu, China
Authors: Ma Yajun , Zhang Ping , Zhao Kaixu , Zhou Yong , Zhao Sidong
Published: June 2022

Making efforts to promote rationalized urban construction land change, distribution, allocation, and its performance is the core task of territory spatial planning and a complex issue that the government must face and solve. Based on the Boston Consulting Group matrix, a decoupling model, and a GIS tool, this paper constructs a new tool that integrates “dynamic analysis + performance evaluation + policy design” for urban construction land. We reached the following findings from an empirical study of Gansu, China: (1) Urban construction land shows diversified changes, where expansion is dominant and shrink cannot be ignored. (2) Most cities are in the non-ideal state of LH (Low-High) and LL (Low-Low), with a small number in the state of HH (High-High) and HL (High-Low). (3) Urban construction land change and population growth, economic development, and income increase are in a discordant relationship, mostly in strong negative decoupling and expansive negative decoupling. (4) The spatial heterogeneity of urban construction land change and its performance are at a high level, and they show a slow upward trend. Additionally, the cold and the hot spots show obvious spatial clustering characteristics, and the spatial pattern of different indexes is different to some extent. (5) It is suggested that in territory spatial planning Gansu should divide the space into four policy areas—incremental, inventory, a reduction development policy area, and a transformation leading policy area—to implement differentiated management policies and to form a new spatial governance system of “control by zoning and management by class”. The change of urban construction land, characterized by dynamics and complexity, is a direct mapping of the urban growth process. The new tools constructed in this paper will help to reveal the laws of urban development and to improve the accuracy of territory spatial planning in the new era. They are of great theoretical significance and practical value for promoting high-quality and sustainable urban development.
The Effects of Soil Improving Cropping Systems (SICS) on Soil Erosion and Soil Organic Carbon Stocks across Europe: A Simulation Study
Authors: Baartman Jantiene E. M. , Nunes Joao Pedro , van Delden Hedwig , Vanhout Roel , Fleskens Luuk
Published: June 2022

Healthy soils are fundamental for sustainable agriculture. Soil Improving Cropping Systems (SICS) aim to make land use and food production more sustainable. To evaluate the effect of SICS at EU scale, a modelling approach was taken. This study simulated the effects of SICS on two principal indicators of soil health (Soil Organic Carbon stocks) and land degradation (soil erosion) across Europe using the spatially explicit PESERA model. Four scenarios with varying levels and combinations of cover crops, mulching, soil compaction alleviation and minimum tillage were implemented and simulated until 2050. Results showed that while in the scenario without SICS, erosion slightly increased on average across Europe, it significantly decreased in the scenario with the highest level of SICS applied, especially in the cropping areas in the central European Loess Belt. Regarding SOC stocks, the simulations show a substantial decrease for the scenario without SICS and a slight overall decrease for the medium level scenario and the scenario with a mix of high, medium and no SICS. The scenario with a high level of SICS implementation showed an overall increase in SOC stocks across Europe. Potential future improvements include incorporating dynamic land use, climate change and an optimal spatial allocation of SICS.
Boon or Bane? Urban Food Security and Online Food Purchasing during the COVID-19 Epidemic in Nanjing, China
Authors: Liang Yajia , Zhong Taiyang , Crush Jonathan
Published: June 2022

This paper examines the relationship between the rapid growth of online food purchasing and household food security during the first wave of the COVID-19 pandemic in China using the city of Nanjing as a case study. The paper presents the results of an online survey of 968 households in Nanjing in March 2020 focused on their food purchasing behavior and levels of food security during the early weeks of the pandemic. While online food purchasing has increased rapidly in many countries during the COVID-19 pandemic, little research attention has been paid to the relationship between online food purchasing and household food security. This paper provides detailed insights into this relationship in China. The medium- and longer-term food security and other consequences of the pandemic pivot to online food purchasing are a fertile area for future research in China and elsewhere.
Smart Justice in Italy: Cases of Apps Created by Lawyers for Lawyers and Beyond
Authors: Lupo Giampiero , Carnevali Davide
Published: June 2022

The smart city literature states that three levels of institutional layers (regulatory, normative, and cognitive) and four typologies of actors (government, universities, citizens, and the private sector) support private initiative for developing smart technologies. Focusing on the emergent phenomenon of smart apps ideated by lawyers’ private initiatives, this paper acknowledges that other factors, including the ubiquity of mobile technologies and the absence of effective public services provided by public institutions, contribute to the institutional and organizational humus necessary for the creation of intelligent technological proposals. In the light of the organizational theory framework, and based on the analysis of the literature on smart cities and e-justice and on the empirical investigation of two Italian lawyers’ apps (Collega and Anthea), this paper identifies the institutional, organizational, and technological conditions under which smart technologies are being developed in high-regulated public institutions’ contexts as justice systems. The findings of the study described in this paper help integrate the contribution of the literature on the topic.
Efficacy of Ficus sycomorus (Sycamore Fig) Extract on Intestinal Coccidiosis in Experimentally Infected Rabbits
Authors: Dawod Ahmed , Fathalla Said , El-Seedi Hesham R. , Hammad Mohamed A. , Osman Noha , Abosheriba Nagwa , Anis Anis , Shehata Awad A. , Elkhatam Ahmed
Published: June 2022

This study was conducted to investigate the effect of the Ficus sycomorus extract on Eimeria intestinalis in experimentally infected rabbits. For this purpose, forty male 30-day-old rabbits (Blanc de Bouscat) were divided into four groups (n = 10 in each group). Rabbits kept in the first group served as negative control (non-treated-non-infected). Rabbits kept in the second, third, and fourth groups were challenged at 10 weeks old with 3 × 104E. intestinalis sporulated oocysts. The third and fourth groups were treated orally with diclazuril 10% (0.05 mg/kg body weight) and F. sycomorus (100 mg/Kg) for three consecutive days, respectively. The efficacy was assessed based on the growth performance parameters, clinical symptoms, oocyst shedding, histopathological findings, and hematological parameters for 16 days post challenge. The study revealed that rabbits treated with F. sycomorus methanolic extract and diclazuril showed mild clinical symptoms with a significant decrease in oocyst shedding compared with the positive control. Moreover, the diclazuril-treated group showed the highest leukocytic count and the lowest monocytes percentage compared with other groups. Furthermore, the lowest lymphocytes percentage was recorded in the control positive group. Histopathologically, moderate coccidia infestation in the intestinal mucosa and moderate hydropic degeneration of hepatocytes were observed in the diclazuril treated group compared with the negative control. However, mild coccidia infestation in the intestinal mucosa and slight coagulative necrosis of hepatocytes was found in the F. sycomorus treated group. In conclusion, F. sycomorus methanolic extract had promising effects on the live performance, oocyst count, and blood variables, while it possesses adverse consequences on the hepatic tissues. Further studies are required to optimize the dose and extraction method to mitigate its side effects.
Assessment of Subjective Tinnitus Treatment Results Using a Prototype Device for Electrical and Magnetic Stimulation of the Ear-Preliminary Study
Authors: Olszewski Jurek , Bielińska Marzena , Kowalski Andrzej Julian
Published: June 2022

Background: The aim of the study was to evaluate the effectiveness of subjective tinnitus treatment in patients with cochlear sensorineural hearing loss with magnetic ear stimulation using a prototype device. Since the 1970s, studies have been conducted on the use of electrical stimulation of the ear in the treatment of tinnitus. The available literature contains various hypotheses about the influence of electrical stimulation of the ear on tinnitus. Material and Methods: Preclinical studies were performed for 100 patients, 40 women and 60 men (124 ears in total), aged 38–72 years, treated for tinnitus. A subjective assessment of the loudness of tinnitus was performed, and the frequency and intensity as well as hearing threshold were determined using a prototype device for electro-magnetic stimulation of the ear. The treatment cycle consisted of 10 five-minute stimulations performed daily 5 times a week. Results: Before treatment, persistent tinnitus was found in 100 ears (80.6%) and periodic tinnitus in 24 ears (19.4%). Immediately after treatment, persistent tinnitus was present only in 50 ears (40.3%) and periodic tinnitus in 40 ears (32.3%). Complete resolution of tinnitus was noted in 34 ears (27.4%). On the other hand, the examination performed 3 months after the treatment showed persistent tinnitus in 40 ears (32.3%) and periodic tinnitus in 50 ears (40.3%), and complete resolution of tinnitus was recorded in 34 ears (27.4%). Based on the VAS analog scale, there was an improvement in tinnitus in 98 ears (79.0%) immediately after treatment and no improvement in 26 ears (20.0%). The mean VAS scale before treatment was 4.9 points, after treatment it was 2.1 points and 3 months after treatment it was 1.9 points. Conclusions: The preliminary research results show the high effectiveness of magnetic stimulation in the treatment of tinnitus with the use of a prototype device for electromagnetic stimulation of the ear. There was no negative effect of the stimulation on hearing or tinnitus.