bluuebunny/crossref_metadata_2025 huggingface dataset:
Vancomycin modestly attenuates symptom severity during onset of and recovery from exertional heat stroke in mice
Authors: Oki Kentaro , Ward Jermaine A. , Ward Shauna M. , Plamper Mark L. , Henderson Chloe G. , Mayer Thomas A. , Caldwell Aaron R. , Leon Lisa R.
Published: October 2023

Vancomycin prophylaxis lowered core temperature before and during EHS, mitigated EHS-associated rise of hepatic biomarkers and cytokines/chemokines in circulation (particularly at 24 h), and corresponded to inhibited phosphorylation of hepatic c-Jun NH2-terminal kinase on Threonine 183/Tyrosine 185 at 0 and 3 h in conscious, unanesthetized mice. However, vancomycin also induced cecal enlargement suggesting its off-target effects could limit its utility against EHS.
Acute effects of β-hydroxybutyrate on left ventricular function in young, healthy adults
Authors: Oneglia Andrew P. , Young Benjamin E. , Cipher Daisha J. , Zaha Vlad , Nelson Michael D.
Published: October 2023

Interest in ketones as a cardiac 'super fuel' has grown significantly following reports of a marked increase in cardiac output following exogenous ketone administration in heart failure. However, the extent to which this increase in cardiac output is related to changes in cardiac contractility, and dependent on the presence of heart failure, remains incompletely understood. Therefore, we performed a randomized, double-blind, placebo-controlled study of oral ketone ester in young healthy volunteers. Baseline cardiac magnetic resonance imaging was performed and repeated every 15 minutes for 60 minutes after ketone and placebo ingestion to assess changes in left ventricular function. As expected, circulating β-hydroxybutyrate increased rapidly after ketone ingestion, but did not change with placebo (interaction: p<0.001). Consistent with prior investigations, ketone ingestion resulted in an average 1 L/min increase in cardiac output after 60 minutes that did not occur with placebo (interaction: p=0.026). This increase in cardiac output was primarily driven by an increase in heart rate after ketone ingestion (interaction: p=0.018), with only a modest increase in stroke volume (interaction: p=0.037). Changes in left ventricular strain and twist mechanics were limited. Taken together, the increase in cardiac output following an acute elevation in circulating β-hydroxybutyrate is primarily driven by changes in cardiac chronotropy, with minimal inotropic contribution.
Vogt–Koyanagi–Harada Syndrome (VKHS): First Two Cases Reported in Pediatric Age Group in Oman
Authors: Al Hashmi Samiya , Al Habsi Nasra , Al Abrawi Safiya
Published: October 2023

The Vogt–Koyanagi–Harada syndrome (VKHS) is a unique form of granulomatous autoimmune disease that mostly impacts the pigmented tissues of the body. The main feature is bilateral granulomatous panuveitis, which is detected on ophthalmologic examination, along with additional systemic signs such as vitiligo, white hair, neurological involvement, or hearing loss. This study aims to report two cases of Vogt–Koyanagi–Harada syndrome presented in the children age group, which is unusual and very rare, to improve recognition of this disease to avoid complications and delay referral.
Frontiers of Energy Storage Technologies
Authors: Wu Tong , Wang Jin-Wei , Qu Shen , Mi Zhifu , Wei Yi-Ming
Published: October 2023

Energy storage technologies (ESTs) play a crucial role in ensuring energy security and addressing the challenges posed by climate change. They enable us to overcome the mismatch between energy supply and demand caused by the intermittent and unpredictable nature of renewable energy sources. The identification of research frontiers in ESTs has primarily relied on expert experience and has been limited to specific areas of study. However, there is a relative lack of data-driven approaches to identify these frontiers. In this study, we employed an integrated technique combining bibliographic coupling and sliding window analysis to identify the research frontiers in ESTs and understand their evolution over time. Our study reveals 19 research frontiers in ESTs distributed across four knowledge domains: electrochemical energy storage, electrical energy storage, chemical energy storage, and energy storage systems. Among these frontiers, two noteworthy areas are aqueous zinc batteries (AZBs) and two-dimensional transition metal carbon-nitride composites (MXenes). By identifying these research frontiers, our study provides insights into the potential future directions for research and development (R&D) deployment in energy storage technologies.
Omega-3 Fatty Acids Inhibit Endoplasmic Reticulum (ER) Stress in Human Coronary Artery Endothelial Cells
Authors: Warda Firas , Graham Lauren , Batch-Joudi Jennifer , Gupta Nidhi , Haas Michael J. , Mooradian Arshag D.
Published: October 2023

The biological effects of fatty acids differ by their structure. Saturated fatty acids and trans-fatty acids are recognized as promoters of coronary artery disease (CAD), while monounsaturated and omega-3 fatty acids may have salutary effects. Since cellular stress is recognized as a fundamental driver of CAD, the effect of these fatty acids on endoplasmic reticulum (ER) stress in human coronary artery endothelial cells (HCAECs) was measured using the ER stress-responsive alkaline phosphatase (ES-TRAP) assay. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and eicosapentaenoic acid ethyl ester (EPA-EE) suppressed ER stress induced with pharmacologic (tunicamycin) and physiologic (high-dextrose concentration) ER stress inducers. In tunicamycin-treated cells, DHA reduced the expression of unfolded protein response (UPR) markers such as phosphorylation of inositol requiring enzyme 1α (IRE1α) and protein kinase R-like endoplasmic reticulum kinase (PERK) and increased activating transcription factor 6 (ATF6) and glucose regulated protein 78 (GRP78) expression. Similarly, treatment with both oleic acid and arachidonic acid, but not elaidic acid (a trans-fatty acid), suppressed both tunicamycin and high-dextrose-induced ER stress while treatment with saturated fatty acids (C14 : 0, C16 : 0, and C18 : 0) enhanced both tunicamycin and high-dextrose-induced ER stress. The latter fatty acids at higher concentrations caused cytotoxicity. These results indicate that omega-3 fatty acids as well as select unsaturated fatty acids and arachidonic acid suppress ER stress in HCAEC.
Nash Equilibrium of Stochastic Partial Differential Game with Partial Information via Malliavin Calculus
Authors: Zong Gaofeng
Published: October 2023

In this article, we consider the Nash equilibrium of stochastic differential game where the state process is governed by a controlled stochastic partial differential equation and the information available to the controllers is possibly less than the general information. All the system coefficients and the objective performance functionals are assumed to be random. We find an explicit strong solution of the linear stochastic partial differential equation with a generalized probabilistic representation for this solution with the benefit of Kunita’s stochastic flow theory. We use Malliavin calculus to derive a stochastic maximum principle for the optimal control and obtain the Nash equilibrium of this type of stochastic differential game problem.
Effect of Caesalpinia decapetala on the Dry Sliding Wear Behavior of Epoxy Composites
Authors: Biratu Hailemariam , Gelaw Mengistu , Shahapurkar Kiran , Chenrayan Venkatesh , Soudagar Manzoore Elahi M. , Tirth Vineet , Algahtani Ali , Al-Mughanam Tawfiq
Published: October 2023

The present research investigates the wear characteristics of an epoxy composite reinforced with a novel Caesalpinia decapetala (CD) shell. The CD is available abundantly worldwide, especially in Ethiopia, particularly in East and West Oromia near West Harar. The composite specimens were processed in the open mould casting technique by varying the vol.% of CD in 10, 20, and 30. EDS is used to evaluate the important elements present in the CD. The density of composites increases with the increase in the content of CD, while the void content estimations reveal good control over the composite fabrication. The wear response of composites is investigated by varying the sliding distance and load and by maintaining a fixed velocity (5 m/s). At a 5 km slide distance and 50 N load, the 30 vol.% Caesalpinia decapetala composition depicts better wear resistance and friction coefficient than other compositions. Experimental results are used to envisage the ideal wear factors and to assess the influence of parameters over the two wear objectives, wear rate and CoF. The grey relational analysis- (GRA-) coupled artificial neural network (ANN) hybrid technique was employed for the prediction and validation. It has been observed that a trivial error of 0.49% amidst GRA and ANN estimation is observed.
Prevalence of Sleep-Disordered Breathing in Prader–Willi Syndrome
Authors: Abushahin Ahmed , Al-Naimi Amal , Abu-Hasan Mutasim , Arar Rania , Lina Hayati M. , Belavendra Antonisamy , Janahi Ibrahim A.
Published: October 2023

Introduction. Sleep-disordered breathing (SDB) is common in patients with Prader–Willi Syndrome (PWS). However, the prevalence of SDB varies widely between studies. Early identification of SDB and factors contributing to its incidence is essential, particularly when considering growth hormone (GH) therapy. Objectives. The aims of the study were to describe the prevalence and phenotypes of sleep-disordered breathing (SDB) in patients with Prader–Willi syndrome (PWS) and to determine the effects of age, gender, symptoms, GH therapy and body mass index on SDB severity. Methods. This study was a retrospective chart review of all patients with genetically confirmed Prader–Willi syndrome who underwent diagnostic overnight polysomnography (PSG) in the sleep laboratory at Sidra Medicine. Clinical and PSG data of enrolled patients were collected. Results. We identified 20 patients (nine males, eleven females) with PWS who had overnight sleep polysomnography (PSG) at a median age (IQR) of 5.83 (2.7–12) years. The median apnea-hypopnea index (AHI) was 8.55 (IQR 5.8–16.9) events/hour. The median REM-AHI was 27.8 (IQR 15–50.6) events/hour. The median obstructive apnea-hypopnea index (OAHI) was 7.29 (IQR 1.8–13.5) events/hour. The median central apnea-hypopnea index (CAHI) was 1.77 (IQR 0.6–4.1) events/hour. Nineteen patients (95%) demonstrated SDB by polysomnography (PSG) based on AHI ≥1.5 events/hour. Nine patients (45%) were diagnosed with obstructive sleep apnea (OSA). Three patients (15%) were diagnosed with central sleep apnea (CSA). Seven patients (35%) were diagnosed with mixed sleep apnea. No correlations were observed between AHI and age, gender, BMI, symptoms, or GH therapy. However, REM-AHI was significantly correlated with BMI (

P
=
0.031

). Conclusion. This study shows a high prevalence of SDB among our patients with PWS. Obstructive sleep apnea was the predominant phenotype. BMI was the only predictor for high REM-AHI. Further studies of large cohorts are warranted to define SDB in PWS and design the appropriate treatment.
Supplementary Table 3 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Myeloid staining panel for the syngeneic Sa1N study.
Supplementary Table 1 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Staining panels for the humanized mouse model experiment.
Supplementary Figure 9 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Gating strategy for evaluating M1 and M2 macrophage and T cell activation markers in dissociated tumors from NGS-SGM3-BLT humanized mice.
Supplementary Figure 6 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Evaluation of activation conditions on multiple primary cells and cell lines.
Supplementary Figure 4 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Characterization of Anti-PSGL-1.
Supplementary Figure 3 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Phenotype of M1, M0 and M2c macrophages.
Data from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Authors: Kauffman Kevin , Manfra Denise , Nowakowska Dominika , Zafari Mohammad , Nguyen Phuong A. , Phennicie Ryan , Vollmann Elisabeth H. , O'Nuallain Brian , Basinski Sara , Komoroski Veronica , Rooney Kate , Culyba Elizabeth K. , Wahle Joseph , Ries Carola , Brehm Michael , Sazinsky Steve , Feldman Igor , Novobrantseva Tatiana I.
Published: October 2023

Abstract
The immune suppressive microenvironment is a major culprit for difficult-to-treat solid cancers. Particularly, inhibitory tumor-associated macrophages (TAM) define the resistant nature of the tumor milieu. To define tumor-enabling mechanisms of TAMs, we analyzed molecular clinical datasets correlating cell surface receptors with the TAM infiltrate. Though P-selectin glycoprotein ligand-1 (PSGL-1) is found on other immune cells and functions as an adhesion molecule, PSGL-1 is highly expressed on TAMs across multiple tumor types. siRNA-mediated knockdown and antibody-mediated inhibition revealed a role for PSGL-1 in maintaining an immune suppressed macrophage state. PSGL-1 knockdown or inhibition enhanced proinflammatory mediator release across assays and donors in vitro. In several syngeneic mouse models, PSGL-1 blockade alone and in combination with PD-1 blockade reduced tumor growth. Using a humanized tumor model, we observed the proinflammatory TAM switch following treatment with an anti-PSGL-1 antibody. In ex vivo patient-derived tumor cultures, a PSGL-1 blocking antibody increased expression of macrophage-derived proinflammatory cytokines, as well as IFNγ, indicative of T-cell activation. Our data demonstrate that PSGL-1 blockade reprograms TAMs, offering a new therapeutic avenue to patients not responding to T-cell immunotherapies, as well as patients with tumors devoid of T cells.
Significance:
This work is a significant and actionable advance, as it offers a novel approach to treating patients with cancer who do not respond to T-cell checkpoint inhibitors, as well as to patients with tumors lacking T-cell infiltration. We expect that this mechanism will be applicable in multiple indications characterized by infiltration of TAMs.
Thalidomide for the Management of Gastrointestinal Bleeding in a Palliative Care Setting
Authors: Fabian Elisabeth , Königsbrügge Oliver , Krejs Guenter J. , Unseld Matthias
Published: October 2023

Background: Palliative care patients frequently present with clinically significant gastrointestinal bleeding. Due to the existence of confounding comorbidities and a remarkably reduced state of general health in many cases, the management of gastrointestinal bleeding in this population is often challenging. Summary: This review summarizes and discusses the role of thalidomide in gastrointestinal bleeding with a special focus on palliative care patients. In addition, an illustrative case report is presented. Thalidomide may be beneficial in gastrointestinal bleeding by exerting antiangiogenic effects. The drug has an acceptable safety profile. Side effects like neurotoxicity may limit its use but can be monitored safely. Due to thalidomide’s thrombin generation potential, patients managed with thalidomide-containing regimes should be closely monitored for deep venous thrombosis. Given its teratogenicity, thalidomide should not be administered to women of childbearing potential who are not using adequate contraception. Key Message: Physicians caring for patients in a palliative care setting should be aware of thalidomide as an effective therapeutic option when endoscopy fails to find a bleeding source or for those patients who cannot or refuse to undergo endoscopy but present with recurrent or obscure gastrointestinal bleeding.
Combined Casein Phosphopeptide-Amorphous Calcium Phosphate and Fluoride Is Not Superior to Fluoride Alone in Early Carious Lesions: A Meta-Analysis
Authors: Golzio Navarro Cavalcante Bianca , Schulze Wenning Alexander , Szabó Bence , László Márk Czumbela , Hegyi Péter , Borbély Judit , Németh Orsolya , Bartha Károly , Gerber Gábor , Varga Gabor
Published: October 2023

There is a growing need for effective methods in the management of early stage carious lesions. Therefore, the aim of this study was to evaluate the effect of combined casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride on white spot lesions (WSLs) compared to fluoride-only interventions. This meta-analysis was performed according to PRISMA guidelines and registered in PROSPERO (CRD42021286245). The Medline, Embase, and Cochrane Central databases were searched until October 17, 2022. Eligible studies were randomized controlled trials. Outcome variables included laser fluorescence (LF), quantitative light-induced fluorescence (QLF), and lesion area scores. The random-effects model was used for analysis, and results were given as standardized mean difference (SMD) and mean difference (MD) with a 95% confidence interval. Risk of bias was assessed using the RoB 2 tool, and the level of evidence with GRADE. Our systematic search yielded 973 records after duplicate removal, 21 studies were included for qualitative synthesis, and 15 studies were eligible for quantitative analysis. No significant difference was found between CPP-ACP and fluoride versus fluoride alone in LF at 1, 3, and 6 months of use: SMD −0.30 (−0.64; 0.04); SMD –0.47 (−1.02; 0.07); SMD –0.49 (−1.13; 0.15), respectively. For QLF, the analysis did not demonstrate significant differences between these two kinds of treatment at 1 and 6 months of use: MD 0.21 (−0.30;0.71); MD 0.60 (−1.70;2.90), but at 3 months, higher QLF values were found in the fluoride-only group compared to the CPP-ACP and fluoride combination was shown regarding the WSLs: MD 0.58 (0.25;0.91). On the contrary, data showed a small but statistically significant decrease in the lesion area in favor of the CPP-ACP plus fluoride versus fluoride alone at 6 months MD –0.38 (−0.72; −0.04). None of these observed changes indicated substantial clinical relevance. The combination of CPP-ACP and fluoride did not overcome the effect of fluoride given alone. Our data suggest that fluoride itself is effective in improving WSLs. However, the certainty of evidence was very low. These results indicate that further studies and future development of more effective products than CPP-ACP are needed in addition to fluoride to achieve robust amelioration of WSLs.
Efficacy of a Self-Directed Video-Based Caregiver-Implemented Language Programme
Authors: Qi Xin , Ng Winnie W.H. , Tsang Gigi H.K. , To Carol K.S.
Published: October 2023

Introduction: Caregiver-implemented language programmes are effective for alleviating early language difficulties. This study examined the efficacy of a self-directed video-based caregiver-implement language programme in Chinese families. Method: This study consisted of two stages. In stage 1, 31 caregiver-child dyads (typically developing children) completed the training programme (group 1) in the form of six video-based training modules. In stage 2, 28 caregiver-child dyads (children with language difficulties) receiving active speech therapy were randomly assigned to the training (group 2) and control arms (group 3). Group 2 received the same training as group 1 in addition to their regular therapy while group 3 was kept as status quo. Caregivers completed a quiz on their knowledge of language facilitation techniques (LFTs) and submitted caregiver-child interaction videos at the start and end of the training. Outcome measures included programme completion rate, quiz scores, and use of LFTs and children’s communication skills in the videos. A pre-post design and a between-group design were adopted in the stage 1 and 2 studies, respectively. Results: A completion rate of about 60% in both stages was noted. Significantly higher post-training knowledge scores were found in groups 1 and 2. General but nonsignificant growth in use of parallel talk and gesture, and significant gains in children’s vocalization in the training arm were observed. Conclusion: The self-directed video-based training programme would be useful in imparting information to caregivers. However, the modest improvements in the use of LFTs suggested direct coaching appeared to still play a significant role in enhancing the actual implementation of LFTs. Further investigation on a larger scale is required to evaluate the effectiveness of the training programme for promoting the wider use of this mode as a preventive measure.
Breakfast Skipping and Determinant Factors among Moroccan School Adolescents (12–19 Years): The Case of Beni Mellal City
Authors: Kaoutar Kamal , Chetoui Ahmed , Boutahar kaltoum , El Moussaoui Soufiane , El Kardoudi Abdeslam , Najimi Mohamed , Chigr Fatiha
Published: October 2023

Introduction: Breakfast skipping is an unhealthy eating behavior reportedly common among adolescents. The aim of the present study was to determine the prevalence and factors associated with breakfast skipping among schoolchildren aged 12–19 years in Beni Mellal city, Morocco, and to assist in the design of interventions to improve breakfast consumption habits of school-aged children in this region. Methods: A school-based cross-sectional study was carried out. A total of 550 children were included in the study. Multivariable logistic regression analysis was used to identify factors associated with skipping breakfast consumption. The association between dependent and independent variables was assessed using an odds ratio (OR) with a 95% confidence interval and p value <0.05 was considered statistically significant. Results: Among 550 Moroccan adolescents evaluated, 25.6% reported not consuming breakfast every day. Breakfast eating was statistically associated with gender (OR = 7.13 [95% CI 1.32–3.84], p = 0.008); mother’s low educational level (OR = 2.86 [95% CI 1.89–4.71], p = 0.091); nonworking mothers (OR = 13.71 [95% CI 1.16–5.29], p < 0.001); not eating between meals (OR = 15.49 [95% CI 2.15–5.49], p < 0.001); eating dinner regularly each day (OR = 17.79 [95% CI 2.81–8.01], p = 0.005); eating vegetables 1–3 times per week (OR = 10.21 [95% CI 1.44–4.67], p = 0.001); consuming milk and dairy product 1–3 times per week (OR = 13.88 [95% CI 1.59–5.56], p < 0.001); and never consuming sodas and soft drink in week (OR = 3.12 [95% CI 1.90–4.50], p = 0.003). Conclusions: This study revealed that the prevalence of skipping breakfast consumption among school-aged children in Beni Mellal city was high. Thus, the findings suggest a community health education program, conducted by school nurses, nutritionists, and other health professionals, is needed.
Innovation and Thought Leadership by Women in Healthcare during the COVID-19 Pandemic
Authors: Ghamri Amani , Zedan Haya S.
Published: October 2023

Introduction: The purpose of this study was to explore the innovation and thought leadership of women in healthcare during the COVID-19 pandemic. Methods: We used a phenomenological qualitative design. Participants were women health leaders (WHLs) from multiple hospital administration levels. Their thought leadership profile and innovation were assessed. Results: All WHLs indicated awareness of strengths and weaknesses, actively prioritised tasks, showed openness to feedback, and effectively assessed sources of conflict. Most were willing to listen to others, accept advice, and open to change. They were confident of their credibility and comprehensibility of their messages, felt capable of solving problems and handling conflict, utilised a negotiating style, and showed situation-appropriate emotion. They were confident in managing teamwork, allocated resources efficiently, and supported employee work preferences. Conclusion: This study provides evidence of WHLs’ capabilities in leading healthcare organisations during crisis and the shift to virtual care in Saudi Arabia. Establishing women’s leadership programs that focus on placing women in healthcare executive positions is necessary to utilise their untapped potential. Facilitating research on women’s leadership capacity, administrative skill, and impact on providing the highest quality healthcare would be valuable. Evaluating infrastructure and preparedness of healthcare organisations to expand virtual care and the potential for innovation to sustain performance during crises are crucial. Supportive hospital culture provides confidence to WHLs to manage and achieve organisational goals with relative fluidity.
Alcohol and Nicotine Dependence and Time to Death in a General Adult Population: A Mortality Cohort Study
Authors: John Ulrich , Rumpf Hans-Jürgen , Hanke Monika , Meyer Christian
Published: October 2023

Introduction: Findings from general population studies are lacking in regard to the co-occurrence of alcohol and nicotine dependence in relation to later mortality. The aim of this study was to analyze potential interactions of risky alcohol drinking, tobacco smoking, alcohol and nicotine dependence, and time until the first cigarette is smoked in the morning after awakening in the prediction of mortality. Methods: This study analyzed a random sample of the general population in Northern Germany, which comprised adults aged 18–64 years. Risky alcohol drinking, tobacco smoking, alcohol and nicotine dependence, and the time until the first cigarette in the morning after awakening were assessed for the period of 1996–1997 by applying the Munich-Composite International Diagnostic Interview. Data about all-cause mortality were gathered for the period of 2017–2018 and analyzed using Cox proportional hazards models. Results: Risky alcohol drinking, tobacco smoking, alcohol and nicotine dependence, and the time until the first cigarette in the morning were associated with each other and predicted the time to death. Among participants with a former alcohol dependence, 29.59% had a current nicotine dependence. Participants who had ever been dependent on alcohol at some point in their life before and currently smoked their first cigarette in the morning within 30 min or less after awakening had a hazard ratio of 5.28 (95% confidence interval: 3.33–8.38) for early death compared to low-risk alcohol consumers who had never smoked. Conclusion: Risky alcohol drinking, tobacco smoking, alcohol and nicotine dependence, and the time until the first cigarette in the morning may have a cumulative impact on time to death. The findings suggest that it could be beneficial to provide support for quitting both risky alcohol drinking and tobacco smoking among nondependent individuals in addition to supporting remission from dependence.
Akne vulgaris: Neue Studie bestätigt Wirksamkeit und Sicherheit der Lasertherapie
Authors: Schulz Andrea
Published: October 2023

Background: Selective photothermolysis on sebaceous glands is an effective method for treating acne vulgaris (AV); however, safety, efficacy, and discomfort hinder its utilization in clinical settings. Aims: The primary objective is to evaluate the safety and efficacy of a novel 1726 nm laser with contact cooling to treat AV. Methods: Seventeen patients aged 18 to 36 were enrolled and treated in this IRB-approved, single-center, open-label study. Patients received up to three facial laser sessions up to seven weeks apart. Follow-up visits happened ten days post-session and at the 4 and 12 weeks following the final session. The investigator assessed the severity of device-related adverse events (AEs). Investigator Global Assessment (IGA) and inflammatory lesion counts (ILC) were used as metrics to evaluate acne resolution and skin condition enhancement. Patients' perspectives on satisfaction and comfort using this technology were assessed using Subject Experience Questionnaires (SEQ). Results: Safety assessment showed mild and transient AEs. All subjects tolerated anesthetics-free treatments well, with a mean treatment discomfort score of 4.9 ± 1.5. Compared to baseline, a statistically significant reduction in ILC (p = 0.003) of 52% to 56% is achieved four to twelve weeks following treatment. Long-term follow-ups showed progressive improvement 24 months post-treatment with a 97% reduction in ILC. SEQs revealed high subject satisfaction (71%) with psychosocial improvement three months post-treatment.
Comorbidities of Chronic Prurigo: A Systematic Review and Meta-Analysis
Authors: Yin Mengting , Yang Chener , Guo Yang , Yang Xu , Dou Xia
Published: October 2023

Background: Chronic prurigo (CPG) is an inflammatory skin disease. Comorbidities including dermatological, cardiovascular, and psychiatric diseases have been reported in patients with CPG; however, the evidence has not been systematically evaluated. We aim to summarize the comorbidities, discuss underlying pathogenesis, and highlight the evaluation of CPG patients. Methods: We performed a systematic search using PubMed, Embase, and Web of Science databases for all articles reporting possible associated diseases with CPG. Pooled random-effects odds ratios (ORs) with 95% CI were calculated. Results: A total of 17 studies were included in this systematic review. Statistically significant association (p <0.05) with CPG has been demonstrated with atopic diseases: atopic dermatitis (pooled OR, 10.91; 95% CI, 3.65–32.67), allergic rhinitis (2.66; 1.12–6.27), asthma (3.23; 1.55–6.74); infectious diseases: hepatitis B (pooled OR, 2.15; 95% CI, 1.11–4.14); endocrine diseases: diabetes (pooled OR, 4.93; 95% CI, 1.13–21.56), type 1 diabetes (2.46; 2.16–2.81), type 2 diabetes (1.89; 1.34–2.68), hyperlipoproteinemia (2.90; 1.61–5.22); cardiovascular diseases: heart failure (pooled OR, 4.13; 95% CI, 1.15–14.91), hypertension (3.17; 1.56–6.45); respiratory system diseases: chronic obstructive pulmonary disease (pooled OR, 3.19; 95% CI, 1.42–7.16); urinary system diseases: chronic kidney disease (pooled OR, 4.16; 95% CI, 1.79–9.66); digestive system disease: inflammatory bowel disease (pooled OR, 2.06; 95% CI, 1.26–3.36); and others: osteoporosis (pooled OR, 3.08; 95% CI, 1.70–5.59), thyroid disease (1.70; 1.17–2.47). Conclusion: CPG is associated with various systemic disorders. Recognition of comorbidities is critical to the appropriate management of affected patients.
Predictive Value of the International Classification of Diseases, 9th Revision Codes for Identifying Ocular Oncology Diagnoses
Authors: Wang Kenny Y. , Xu Timothy T. , White Launia J. , Dalvin Lauren A.
Published: October 2023

Introduction: The aim of this study was to determine the predictive value of International Classification of Diseases, 9th Revision (ICD-9) billing codes for identifying ocular oncology diagnoses. Methods: Population-based retrospective cohort study of all Olmsted County, Minnesota residents with any ocular neoplasm-related ICD-9 code from January 1, 2006 to October 1, 2015. All medical records were reviewed for confirmation of ocular neoplasm. Diagnoses with ≥5 cases confirmed via a medical record review were compared to corresponding ICD-9 codes. Main outcome measures included positive predictive value (PPV), negative predictive value (NPV), sensitivity, and specificity of ICD-9 codes. Results: Among 3,932 subjects with ≥1 ocular neoplasm-related ICD-9 code, 21 diagnoses met study criteria. The most frequent intraocular, extraocular/orbital, and ocular surface diagnoses were choroidal nevus (n = 824), epidermal inclusion cyst (n = 263), and conjunctival nevus (n = 74), respectively. PPVs ranged from 1.2% to 73.8%, NPVs from 96.9% to 100%, sensitivity from 0% to 100%, and specificity from 85.7% to 100%. Among malignant neoplasms, PPV ranged from 0% to 73.8%: ocular surface squamous neoplasia (PPV: 0%), choroidal melanoma (PPV: 25.0%), eyelid squamous cell carcinoma (PPV: 46.7%), and eyelid basal cell carcinoma (PPV: 73.8%). Among benign neoplasms, PPV ranged from 1.2% (dermoid cyst) to 61.6% (choroidal nevus). Conclusion: There was a wide variation in a predictive value of ocular neoplasm-related ICD-9 billing codes, which suggests that ocular oncology-related claims data alone may overestimate the true number of ocular oncology diagnoses.
The Effect of Month-Long Daily Fasting on Semen Parameters: A Retrospective Cohort Study
Authors: Engel Offra J. , Doctory Neta , Sun Bei , Miller Netanella , Levi Matan , Schreiber Hanoch , Samara Nivin , Wiser Amir , Haikin Herzberger Einat
Published: October 2023

Objective: The aim of the study was to determine whether Ramadan month-long daily fasting affects semen analysis parameters. Methods: This retrospective cohort study was conducted in tertiary academic medical center. Medical records of 97 Muslim patients who were admitted to the IVF unit from May 2011 to May 2021 were reviewed. Only men who provided at least one semen sample during Ramadan period (Ramadan month +70 days after) and one sample not during Ramadan were included. Semen characteristics of each patient were independently compared to themselves. Results: The post-gradient semen analysis indicated significantly lower progressive sperm motility (mean 30.01 ± 20.46 vs. 38.12 ± 25.13) (p < 0.001). The decrease in the progressive motility remained significant among patients with non-male factor indications (p < 0.001). In the non-male factor indication group, the difference in the progressive motility of the post-gradient semen analysis between the 2 samples was not statistically significant (p = 0.4). There were no significant differences between semen parameters before centrifuging. The incidence of asthenospermia (progressive sperm motility <32%) as an absolute parameter was higher after centrifuging the semen sample during the Ramadan period (p = 0.04). Conclusions: Semen samples collected during Ramadan period were associated with lower progressive motility and reduced semen volume compared to semen samples from the same men outside of the Ramadan period. A possible effect of these altered semen parameters on fertility should be investigated further.
Inovasi Motor Listrik Untuk Alat Penggilingan Kacang Tanah
Authors: Rifaldi Rifaldi , Kun Fayakun  , Rosalina Rosalina , Emilia Roza  , Ramza Harry
Published: October 2023

Penelitian ini bertujuan untuk merancang sistem kontrol Penggiling Kacang Tanah berbasis Motor AC 135 Watt, yang ditujukan untuk membantu para pengusaha UMKM di bidang pangan yang menggunakan bahan baku kacang tanah. Tujuan dari alat ini adalah untuk meningkatkan efisiensi produksi dalam pembuatan bahan baku bumbu kacang dengan menggantikan proses manual yang memakan waktu lama menjadi sistem otomatis yang dapat memilah kacang tanah menjadi tekstur halus dan kasar sesuai keinginan. Dengan menggunakan Motor AC 135 Watt yang didapatkan dari mesin cuci sebagai komponen penggerak utama, rancangan ini bertujuan untuk mengurangi biaya produksi dengan memanfaatkan kembali komponen yang sudah ada. Sistem kontrol akan mencakup mekanisme untuk menggiling kacang tanah dengan efisien, memastikan keselamatan pengguna, dan mengatur kecepatan motor serta durasi penggilingan. Penelitian ini juga menekankan pada ramah lingkungan dengan mendaur ulang komponen yang tidak terpakai. Secara keseluruhan, Penggiling Kacang Tanah yang diusulkan bertujuan untuk memberikan manfaat bagi para pengusaha UMKM dengan menyederhanakan proses produksi dan meningkatkan produktivitas dalam persiapan bahan baku bumbu kacang.
Seasonality, diversity, and forest type associations of macro moths (Insecta: Lepidoptera: Heterocera) in the Shiwalik landscape of northern India and its conservation implications
Authors: Singh Arun Pratap , Lekhendra
Published: October 2023

A study was carried out to evaluate the seasonal diversity of macro moths across different forest sub-types occurring in theShiwalik landscape of northern India, mainly Uttarakhand and adjoining states of Himachal Pradesh, Uttar Pradesh, and Haryana. Fortythree field surveys of 59 days were carried out from July 2020 to October 2022 using stratified random sampling in each of the 19 selected study sites. Sampling surveys revealed 321 species of moths belonging to 19 families and 49 sub-families. These new range extensions from central Himalaya and northeastern India indicate the affinity of moths found in the northern Indian Shiwaliks with that of the Oriental region. Seasonal trend of species richness showed two annual peaks, with the first peak occurring in August followed by a smaller peak in October, while the seasonal abundance of moth species was maximum in July followed by a smaller peak in September. One-hundredand-forty species occurred only during the ‘monsoon’ season indicating their seasonality, univoltine habit and short flight periods in these tropical forests. Species richness of moths correlated positively with relative humidity (r2 = 0.100; p = 0.0142; n = 59). The most dominant family was Erebidae (95 species) followed by Geometriidae (61), Crambidae (72), and Noctuidae (28), respectively. Maximum number of moth species were sampled in forest sub-type (i) 3C/C2a Moist Shiwalik Sal Forest, followed by (ii) 5B/C2 Northern Dry Mixed Deciduous Forest, (iii) 3C/C2c Moist Tarai Sal Forest, iv) 5B/C1a Dry Shiwalik Sal Forest, respectively. The study also revealed changing moth communities along with the vegetation structure in the Shiwaliks from east (Nandhaur Willife Sanctaury in Uttarakhand bordering Nepal) to west (Simbalbara National Park in Himachal Pradesh,India) across the landscape. The moth communities of (i) 3C/C2a Moist Shiwalik Sal Forest & (ii) 3C/C2c Moist Tarai Sal Forest being different from that of (iii) 5B/C2 Northern Dry Mixed Deciduous Forest, and (iv) 5B/C1a Dry Shiwalik Sal Forest. Besides, six new range extensions into Shiwaliks of northern India from central Nepal and northeastern India, namely: Chlorozancla falcatus (Butler, 1889) (Geometridae); Cynaeda dichroalis (Hampson, 1903), Dichocrocis pyrrhalis (Walker, 1859) & Glyphodes canthusalis Walker, 1859 (Crambidae); and Acropteris iphiata (Guenée, 1857) (Uranidae) were recorded.
Aggf1
Specifies Hemangioblasts at the Top of Regulatory Hierarchy via
Npas4l
and mTOR-S6K-Emp2-ERK Signaling
Authors: Yang Zhongcheng , Guo Di , Zhao Jinyan , Li Jia , Zhang Rui , Zhang Yidan , Xu Chengqi , Ke Tie , Wang Qing K.
Published: October 2023

BACKGROUND:

Hemangioblasts are mesoderm-derived multipotent stem cells for differentiation of all hematopoietic and endothelial cells in the circulation system. However, the underlying molecular mechanism is poorly understood.

METHODS:

CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (type II CRISPR RNA-guided endonuclease) editing was used to develop
aggf1

−/−

and
emp2

−/−

knockout zebra fish. Whole-mount in situ hybridization and transgenic Tg(
gata1
-EGFP [enhanced green fluorescent protein]), Tg(
mpx
-EGFP), Tg(
rag2
-DsRed [discosoma sp. red fluorescent protein]), Tg(
cd41
-EGFP), Tg(
kdrl
-EGFP), and Tg(
aggf1

−/−

;
kdrl
-EGFP) zebra fish were used to examine specification of hemangioblasts and hematopoietic stem and progenitor cells (HSPCs), hematopoiesis, and vascular development. Quantitative real-time polymerase chain reaction and Western blot analyses were used for expression analysis of genes and proteins.

RESULTS:

Knockout of
aggf1
impaired specification of hemangioblasts and HSPCs, hematopoiesis, and vascular development in zebra fish. Expression of
npas4l
/
cloche
—the presumed earliest marker for hemangioblast specification—was significantly reduced in
aggf1

−/−

embryos and increased by overexpression of
aggf1
in embryos. Overexpression of
npas4l
rescued the impaired specification of hemangioblasts and HSPCs and development of hematopoiesis and intersegmental vessels in
aggf1

−/−

embryos, placing
aggf1
upstream of
npas4l
in hemangioblast specification. To identify the underlying molecular mechanism, we identified
emp2
as a key
aggf1
downstream gene. Similar to
aggf1
,
emp2
knockout impaired the specification of hemangioblasts and HSPCs, hematopoiesis, and angiogenesis by increasing the phosphorylation of ERK1/2 (extracellular signal-regulated protein kinase 1/2). Mechanistic studies showed that
aggf1
knockdown and knockout significantly decreased the phosphorylated levels of mTOR (mammalian target of rapamycin) and p70 S6K (ribosomal protein S6 kinase), resulting in reduced protein synthesis of Emp2 (epithelial membrane protein 2), whereas mTOR activator MHY1485 (4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine) rescued the impaired specification of hemangioblasts and HSPCs and development of hematopoiesis and intersegmental vessels and reduced Emp2 expression induced by
aggf1
knockdown.

CONCLUSIONS:

These results indicate that
aggf1
acts at the top of
npas4l
and becomes the earliest marker during specification of hemangioblasts. Our data identify a novel signaling axis of Aggf1 (angiogenic factor with G-patch and FHA domain 1)-mTOR-S6K-ERK1/2 for specification of hemangioblasts and HSPCs, primitive and definitive hematopoiesis, and vascular development. Our findings provide important insights into specification of hemangioblasts and HSPCs essential for the development of the circulation system.
In female degus, reunions are less variable when relationships are new
Authors: Thatcher Amber , Insel Nathan
Published: October 2023

When establishing new peer relationships, animals may explore different modes of interaction, testing-out dominance roles, reciprocation of affiliation, and responses to investigation. This exploration is potentially risky, as higher variability may be counterproductive to establishing expectations and trust. There is therefore a tradeoff between exploration within a new social relationship and maintaining predictable, ‘safe’ behaviours, raising questions about how animals differ in how they engage with strangers. The Chilean degu offers an opportune case study to investigate novel social situations, as females form relationships relatively rapidly with unrelated peers. We presented degu dyads with a series of 20 min ‘reunion’ sessions and found that session-to-session variability in stranger females is, in fact, lower than in cagemates, and lower than stranger or cagemate males. Reduced variability was observed only after an initial social exposure, suggesting it was a feature of new relationships rather than novelty. There was no evidence that groups differed in predictability of behaviours within a reunion. It is known that in the wild, female degus differ from males by readily forming cooperative relationships with unrelated individuals. The data therefore raise the possibility that animals predisposed to cooperation might also show reduced behavioural variability across encounters with new individuals. This work offers new results and methods for considering strategies animals use to cope with social uncertainty.
Murmurs and Miracles
Authors: Padgett Isaiah C.
Published: October 2023

This article explores how early Pentecostals read, interpreted, and utilized the character of Miriam within the Old Testament. Despite the prominent focus upon masculine Old Testament figures within pentecostal history, the reception of feminine figures within Pentecostalism is less clear. As such, this article seeks to uncover how Miriam the prophetess was presented and interpreted within early pentecostal literature, with the emphasis on such subjects as divine healing, dancing, support for women in ministry, faithfulness, and murmuring. These interpretations of Miriam demonstrate positive and negative appraisals of her character and reflect her multifaceted reception within Pentecostalism. Finally, this article concludes with a summary of these interpretations and a call for continued reflection upon historic readings within pentecostal history.
Environmental Impact Assessment of Offshore Windfarms in Areas beyond National Jurisdiction: Who Should Have Obligations?
Authors: Seta Makoto
Published: October 2023

After almost two decades of discussions and negotiations, the legally binding instrument on biodiversity beyond national jurisdiction (‘BBNJ’) was adopted on 19 June 2023, of which Environment Impact Assessment (‘EIA’) is one of the key pillars. Given that EIA in the new instrument is proposed with a view to the conservation and sustainable use of marine life in areas beyond national jurisdiction, flag States should mainly have an obligation to regulate EIA by applying their domestic legislation. However, for some activities involving many vessels, marine structures, and cables, EIA by flag States may not work effectively. EIAs for offshore windfarm activities may be particularly difficult. Against this background, this study explores how international legal rules should regulate EIAs of offshore windfarms established in areas beyond national jurisdiction. In particular, this study considers which States should have EIA obligations from the perspective of both lex lata and lex ferenda.