bluuebunny/crossref_metadata_2025 huggingface dataset:
Bovine Genome Analysis to Unravel the Location and Feature of Target Sites of RNA-Guided Hyperactivated Recombinase Gin with Spacer Length Six
Authors: Pathak Shalu Kumari , Sonwane Arvind , Kumar Subodh
Published: January 2022

Background: Programmable nucleases are very promising tools of genome editing (GE), but they suffer from limitations including potential risk of genotoxicity which led to the exploration of safer approach of GE based on RNA-guided recombinase (RGR) platform. RNA-guided recombinase (RGR) platform operates on a typical recognition or target site comprised of the minimal pseudo-core recombinase site, a 5 to 6-base pair spacer flanking it and whole this central region is flanked by two guide RNA-specified DNA sequences or Cas9 binding sites followed by protospacer adjacent motifs (PAMs). Methods: The current study focuses on analysis of entire cattle genome to prepare a detailed map of target sites for RNA-guided hyperactivated recombinase Gin with spacer length six. For this, chromosome wise whole genomic sequence data was retrieved from Ensembl. After that search pattern for recombinase Gin with spacer length six was designed. By using this search pattern, RGR target sites were located by using dreg program of Emboss package. Result: Total number of RGR target sites identified in bovine genome for recombinase Gin was 677 with spacer length six. It was also investigated that whether these RGR target sites are present with in any gene or not and it was found that RGR target sites lies in both genic and intergenic region. Besides this, description of genes in context with these target sites was identified.
​Genital Changes, Haemato-biochemical Profile and Therapeutic Management of Endometritis in Crossbred Dairy Cows
Authors: Dutta L.J. , Deka R. , Nath K.C. , Bhuyan M. , Baruti M. , Bora D.P.
Published: January 2022

Background: Reproductive performance is one of the major determinants for the economic improvement of a dairy farm. Endometritis and other uterine complications cause decrease the pregnancy rate. In this study, the genital changes and haemato-biochemical profile of crossbred dairy cows affected with endometritis was studied to evolve a suitable treatment protocol in order to improve reproductive efficiency. Methods: Crossbred cow affected with endometritis were selected based on mucopurulent vaginal discharge at estrus and tested positive for white side test. Animals were divided randomly into 8 groups with 24 animals in each group. Different therapeutic regimens fortified with supportive treatment were opted. The therapeutic regimens were supportive therapy having bypass fat, vitamins and minerals, Lugol’s iodine, Lugol’s iodine with supportive therapy, intrauterine (IU) antibiotic, IU antibiotic with supportive therapy, E. coli. LPS, E. coli. LPS with supportive therapy and control group respectively. Efficacy of each treatment regimen was based on first service conception rate (FSCR). Result: Of all the therapeutic regimens, fortification of Lugol’s iodine with supportive therapy resulted in higher FSCR (83.33%) indicating better applicability as a treatment tool for endometritis in crossbred cows.
​Nodulation, Yield Attributes and Yield of Mungbean [Vigna radiata (L.)] Influenced by Different Level of Potassium Humate and Fertility Levels
Authors: Kumar Yogesh , Verma Rajhans , Singh Kuldeep , Bhukhar Oma Shanker , . Rajesh
Published: January 2022

Background: Optimum crop growth and yield is result of interlinking of several factors. In semi- tropical soil in central plateau and hills zone are deficit in organic carbon and NPK content; therefore inadequate fertilization may leads to pure quality and also lower crop productive capacity of soil. For the maintenance of sustainable and productive production, maintaining soil health is a critical factor. Under low fertility levels, mungbean gives low seed yield. Potassium humate, nitrogen and phosphorus (RDF) application may be increase yield of mungbean in this zone. Method: A field experiment was conducted to study, “Nodulation, yield attributes and yield of mungbean [Vigna radiata (L.)] influenced by different level of potassium humate and fertility. The experiment was carried out in factorial randomized block design with three replications and sixteen treatment combination. Result: Result showed that total number of root nodules, effective nodules, fresh and dry weight of root nodules, leghaemoglobin, nodule index, no. of pods/plant, no. of seeds/pod, test weight, seed and straw yield were observed significantly higher with application of potassium humate @ 4.5 kg/ha. Among different fertility level, the application of 100% RDF significantly increased the total number of root nodules and effective nodules, fresh and dry weight of root nodules, leghaemoglobin, nodule index, no. of pods/plant, no. of seeds/pod and test weight, seed and straw yield. With combined application of potassium humate @ 3.0 kg/ha + 75% RDF significantly higher no. effective nodules, dry weight of root nodules and seed yield were observed, as well as saving of 25% RDF and 1.5 kg potassium humate were also observed.
Variability and Virulence Analysis of Aspergillus niger Isolates Causing Collar Rot of Groundnut
Authors: Bajaya Tejpal , Ghasolia R.P. , Bajya Mamta , Shivran Manisha
Published: January 2022

Background: Collar rot of groundnut (Arachis hypogaea L.) caused by Aspergillus niger is a significant constraint in groundnut cultivation and responsible for huge economic losses in India including Rajasthan. Methods: By surveying of eight major groundnut growing districts of Rajasthan, India, one representative Aspergillus niger isolate from each district was established (ANBK-01= Bikaner, ANCH-02= Churu, ANDA-03= Dausa, ANJP-04= Jaipur, ANJL-05= Jalore, ANJD-06= Jodhpur, ANNG-07= Nagaur and ANSK-08= Sikar) for studying variability in the pathogen as well as to know the response of groundnut varieties to the highly virulent isolate. The colony and spore characteristics were observed for cultural and morphological variability. For resistance response to the disease, ten varieties (M-13, RG-633-9, RG-382, Girnar-2, RG-604, RG-578, Gajraj 10, RG-510, RG-632-1 and RG-644) were evaluated in the field for two consecutive years against a highly virulent Aspergillus niger (ANJP-04) isolate. Result: Our investigations cleared that all the isolates were showed cultural and morphological variability such as shape, colour and size of colony and size of conidia, conidiophores and columella. Isolate (ANJP-04) collected from Khejroli village of Chomu tehsil in Jaipur district showed maximum mycelial growth, conidia diameter, length and diameter of conidiophores and length and diameter of columella, early sporulation and found most virulent as it produced higher disease incidence (54.43%). Ten released varieties of groundnut in the field conditions, revealed that none of the variety was found completely free from the disease whereas RG-644, M-13 and RG-510 were found resistant while RG-604, Girnar-2, Gajraj-10 and RG-632-1 were found moderately resistant and rest were found susceptible to highly susceptible to the disease. Conclusively, it can be finalized that famers may cultivate these resistant varieties in areas where collar rot is a severe constraint. The conclusion of this study can also be utilized to screen varieties/genotypes of groundnut against highly virulent isolate for sustainability of breeding material to the disease effectively.
Effect of Plant Growth Promoting Bacillus spp. on Germination and Seedling Growth of Soybean
Authors: Miljaković D. , Marinković J. , Tamindžić G. , Đorđević V. , Ignjatov M. , Milošević D. , Nikolić Z.
Published: January 2022

Background: Bacillus spp., known to promote growth and reduce disease of various field and vegetable crops, are frequently found in soils. The objective of the study was to select effective Bacillus spp. isolates with multiple plant growth properties and antifungal activities and to examine their effect on germination of soybean. Methods: Bacterial isolates were screened for production of indole-3-acetic acid (IAA) and siderophores and solubilization of phosphate. The ability of bacterial isolates to inhibit the growth of seven phytopathogenic fungi affecting soybean was determined using a dual plate assay. Bacillus spp. were further selected and examined in a seed germination test. Result: All Bacillus spp. isolates were positive for IAA production, while siderophore production and P-solubilization were observed in 80% and 20% bacterial isolates, respectively. Bacillus spp. exhibited the highest antifungal activity against Diaporthe caulivora, followed by Diaporthe sojae, Diaporthe eres, Diaporthe longicolla and Macrophomina phaseolina and the least antagonistic effect toward Fusarium graminearum and Fusarium subglutinans. Selected isolates of B. subtilis significantly affected final germination, shoot length, root length, shoot dry weight and root dry weight of two soybean cultivars. The most effective Bacillus spp. isolates could be used as potential inoculants for improving soybean productivity.
DITADURA MILITAR E TEORIA QUEER EM STELLA MANHATTAN, DE SILVIANO SANTIAGO
Authors: Penha Oliveira Ligia Vanessa , Albuquerque da Silva Santos Jonas Vinicius
Published: January 2022

A literatura gay no Brasil se iniciou com a polêmica publicação de O Bom Crioulo (1895) do naturalista Adolfo Caminha e, a partir disso, houve um aumento, a passos pequenos, do número de obras literárias que envolvessem a homossexualidade em suas composições. Partindo disso, neste artigo realizamos uma leitura crítica da obra Stella Manhattan, um romance lançado em 1985 pelo autor brasileiro Silviano Santigo, que em sua narrativa apresenta um rapaz gay que é exilado nos Estados Unidos após a instauração da ditadura militar de 1964 e passa a trabalhar no consulado brasileiro, em Nova York. Santiago publicou sua obra após o término do período ditatorial brasileiro e não sofreu consequências graves, pois a homossexualidade era vista como uma doença. Já outros autores, como Cassandra Rios, tiveram suas obras censuradas pelos militares. Assim, intentamos abordar a temática da homossexualidade presente em Stella Manhattan, correlacionando-a com os estudos da Teoria Queer e com o contexto histórico presente no romance de Santiago. A análise aqui realizada baseia-se nos estudos de João Silveiro Trevisan (2018), James N. Green (2018), Judith Butler (2016), dentre outros teóricos.
DIFERENÇAS QUE CARACTERIZAM O DRAMA A STREETCAR NAMED DESIRE E SUA TRADUÇÃO UM BONDE CHAMADO DESEJO
Authors: Cardoso Silveira Gustavo
Published: January 2022

O objetivo deste artigo é a comparação entre o original em língua inglesa do drama A Streetcar Named Desire, de Tennessee Williams, com a tradução em português, Um bonde chamado Desejo, de Vadim Nikitin, a fim de identificar as categorias linguísticas que apresentam diferenças entre as duas versões. Nos anos noventa, o conceito do tradutor subserviente foi substituído por outro, visivelmente manipulador, entendido como um sujeito inserido em um contexto cultural, ideológico, político e psicológico – não podendo ser ignorado ou eliminado. Como resultado dessas imposições, o tradutor é obrigado a fazer escolhas nem sempre fiéis às da língua do texto original, implicando em diferenças na interpretação do texto. A análise tem o apoio da Linguística Sistêmico-Funcional (LSF), uma proposta teórico-metodológica, de Halliday (1994), Halliday; Matthiessen (2004) e seus colaboradores. Como Martin e White (2005, p. 7) explicam, "a LSF é “um modelo multiperspectivo, designado a dar aos analistas lentes complementares para a interpretação da língua em uso". Os resultados da análise mostram a impossibilidade de uma tradução exata e fiel ao original, devido a dificuldades de natureza tanto referentes à subjetividade do tradutor, quanto às características tipológicas das línguas em confronto.
HISTÓRIA E LITERATURA: UM DIÁLOGO POSSÍVEL COM O ROMANCE PIUM DE ELI BRASILIENSE
Authors: Oliveira Maria de Fátima , Freitas Larissa dos Santos
Published: January 2022

O presente artigo busca analisar a relação entre a História e a Literatura no romance Pium, do escritor Eli Brasiliense. O estudo tem como embasamento teórico autores que defendem ser esse diálogo viável e vantajoso para as duas áreas do conhecimento. Contudo, nem sempre essa relação foi um consenso, ficando a literatura à margem das fontes históricas por um longo tempo, mas este cenário foi modificado principalmente a partir da escola dos Annales, que passou a considerá-la importante para investigar com maior amplitude a história e a sociedade, devido a aceitação e inserção de novos objetos, novas abordagens e consequentemente com a aceitação de novas fontes, dando ênfase à História Cultural. A hipótese que se levanta é a de que o diálogo entre a História e a Literatura é muito profícuo na obra de Brasiliense e que por meio do romance Pium é possível conhecer aspectos econômicos, políticos e culturais da região no período.
Opinions about the content of the anatomy course of future physical education teachers and their ability to apply what they have learned in professional activities
Authors: Makhambetov Yerik , Baikenzheyeva Ainur , Kassymov Sultan , Yessirkepov Zhandos , Kurmanbaev Rahat
Published: January 2022

Abstract

The behaviours gained in graduate education should be used in their professional life. As a result of the literature review, it is concluded that physical education teachers have problems in transferring knowledge in their professional competence. The opinions of physical education teachers about the education they received at the university are important in terms of investigating the source of this problem. Anatomy is about the body. The achievements of the anatomy course and the applicability of these gains are very important. This research was conducted with last year students studying at the physical education department at the university. 48 pre-service teachers who took the Anatomy course, studying in the physical education department, participated in the research. Researchers were selected based on volunteerism. The qualitative research method was used in the research and the findings were supported by content analysis. 4 open-ended questions prepared by the researcher were prepared by taking expert opinions. The results obtained from the research show that the anatomy course is important for physical education, and the behaviours to be gained in this course will have to be used frequently in professional life. Again, it was concluded that explaining the anatomy course content as applied while preparing it will facilitate their learning. As a result of the results obtained from this research, the anatomy course taught in universities is an important course for the physical education profession. The method applied for the problems experienced in this course can be changed technically. In the anatomy course, which includes theory and practice, practice can eliminate the problems experienced.

Keywords: physical education, anatomy, professional competence, usability, human structure, training program
Penggunaan Mobile Learning dengan Live Streaming Facebook pada Mata Pelajaran Qur’an Hadith
Authors: Zainiyati Husniyatus Salamah , Muna Nailul
Published: January 2022

This study aims to identify the use of Mobile Learning by using Facebook Live Streaming in the student learning process at MTs Robithotul Ashfiya' Sidokumpul Bungah Gresik for Qur'an Hadith subjects. This is motivated by the activities of the Qur'an Hadith teacher at MTs Robithotul Ashfiya, who are busy outside the madrasa, so most of the teaching and learning process uses bold methods. This study involved students in grades 7 (seven) in grading 9 (nine) and 1 (one) education staff at MTs Robithotul Ashfiya as Madrasah Operators. The research uses an exploratory case study method. The research approach uses qualitative methods to obtain accurate information about the effectiveness of using mobile learning with Facebook live streaming on Qur'an Hadith subjects at Madrasah Tsanawiyah Robithotul Ashfiya' Sidokumpul Bungah Gresik. The results of this study explain that the use of mobile learning with Facebook live streaming on Qur'an Hadith subjects at the Madrasah Tsanawiyah unit level runs effectively.
EPISTEMIC RATIONALITY IN ISLAMIC EDUCATION: The Significance for Religious Moderation in Contemporary Indonesian Islam
Authors: Prasetia Senata Adi , Asrohah Hanun , Najiyah Siti Firqo , Arif Syaiful
Published: January 2022

This article discusses the concept of epistemic rationality in Islamic education and its significance for strengthening religious moderation in contemporary Indonesian Islam. The questions are: (1) How is the conception of epistemic rationality? (2) To what extent is the role of epistemic rationality in Islamic education? (3) How do classical Islamic treasures view the epistemic rationality and its significance for strengthening religious moderation in Indonesian Islam? Afterwards, the theoretical assumption underlying this article is that the strengthening of religious moderation without being supported by epistemic rationality is null and void. This article finds that the passion of reading in Islam processed through epistemic rationality has provided huge contribution for Islamic civilization so that it reaches its golden age. Hence, in Indonesian Islamic education context, strengthening religious moderation must be delivered through epistemic rationality as basic reasoning in understanding religious text and digging the diversity phenomenon in order to avoid radicalism and blind fanaticism. Epistemic rationality must be considered as starting point to build curriculum structure and learning contents that emphasize more on the competency of ‘know-how’ and ‘know-why’ rather than ‘know-what’.
A LITERATURE REVIEW: LOW SODIUM RESTRICTION OF PATIENTS WITH HEART FAILURE ON HOSPITAL READMISSION
Authors: Lumbantoruan Septa Meriana
Published: January 2022

Sodium restriction effect on hospital readmission in patients with heart failure (HF) has been questioned for decades. Readmission related to low sodium intake recommendations should be changed as well as mortality. A literature review is needed to summarize the effect of low sodium, especially on readmission and mortality. This literature review aimed to summarize the prevalence of hospital readmission and mortality regarding low sodium intake in patients with HF. The searching process involved four databases; MEDLINE, Embase, EBSCO Health, Cochrane was explored for experimental studies of sodium restriction. Of 77 screened citations from 2000 to 2019 invested in patients with HF, four studies were included. Four studies from four databases were included and explained and it was found that hospital readmission was the outcome of implementing sodium restriction in patients with HF. Low sodium restriction (800 mg – 1800 mg/day) results in higher hospital readmission. Moreover, 1800 mg/day of sodium was followed by higher mortality and higher sudden death in patients with HF. Low sodium restriction did not lower hospital readmission as well as mortality of patients with HF. This article provides the reason, effect, and amount of sodium restriction in patients with HF. The recommendation from this literature review is low sodium restriction has no beneficial effect on readmission and mortality in HF conditions.
Digitalisasi Pelayanan Publik Desa Palewai Dengan Sistem Informasi Desa
Authors: Bantun Suharsono , Sari Jayanti Yusmah , Z Noorhasanah , Syahrul Syahrul , Budiman Arief
Published: January 2022

One of the basic functions of government is to provide public services. Even during the Covid-19 pandemic, these needs must still be met and optimally adjusted by service providers. The Palewai Village Office, is one of the local government agencies that does not escape its obligation to improve public service standards during this pandemic. However, based on observations, it was found that the performance of public services in the office was not optimal because population data processing was still manual and data storage media still used physical documents. Therefore, an online information system is needed that can provide clear and up-to-date information, as well as make it easier for people to fill out forms from anywhere so that there is no crowd in the service room. This study aims to develop a Website- Based Village Information System at the Palewai Village Office which will handle population data, services for making cover letters and distributing population data and current information for the community. The results of this study are a website-based village information system that can manage community data effectively and efficiently and can be accessed quickly and easily to provide information related to services at the Palewai Village office.
Optimasi K-Klasterisasi Ketahanan Pangan Kabupaten Jember Menggunakan Metode Elbow
Authors: Fuadah Aski Widdatul , Arifin Fajrin Nurman , Juwita Oktalia
Published: January 2022

Clustering is a process of grouping data based on similarities or similarities with other members in a group. Food security is the condition of a country to provide food for individuals, which does not conflict with beliefs, religion and culture and leads a healthy, active and productive life. Food instability and food insecurity can be caused by many factors, one of which is natural disasters. In 2020, Jember Regency experienced 121 natural disasters. Determination of the optimal K value is done to get the right number of group divisions from the clustering process, in this case using the elbow method. The data used in the clustering process are sub-districts in Jember Regency using transient attributes or natural disaster events. Based on the results of sub-district data grouping from the number of clusters k=1 to k=10, the optimal k value was found at the value of k = 4 with the SSE (Sum of Square Error) value = 24,809.
An expanded knowledge production function
Authors: Barros Pedro Henrique Batista de , Freitas Júnior Adirson Maciel de
Published: January 2022

This paper uses a theoretical motivation for an Expanded Knowledge Production Function(EKPF) that encompasses both path dependence and spatial spillovers to search for evidences inBrazil using a Dynamic Spatial Panel Data approach. The purpose is to identify the determinantsof knowledge production in the 2005-2015 period as well as its temporal evolution, usinginnovation patents as proxies. Regarding its spatial distribution, we identified a North-Southdisparity for the knowledge production in Brazil, with Southeast and South producing alarge part of the country’s patents. Based on the EKPF, we confirmed the importance ofpath dependence and knowledge spillovers to explain the Brazilian innovation. In addition,population density, which generates Jacobian externalities and economies of agglomeration, isan important structural feature in the short run while the number of researchers in universitiesand an increased economic scale are essential to knowledge production in the long run.
A infoinclusão da pessoa com deficiência proporcionando acesso igualitário ao mercado de trabalho em tecnologia da informação e comunicação
Authors: Fornaciali Michel Silva , Almeida Lucimara de , Almeida Luis Rogerio Gomes de
Published: January 2022

A inclusão das pessoas com deficiência (PcD) no mercado de trabalho vem aumentando, assim como a consciência social dos empregadores, os quais abrem oportunidades igualitárias para a ocupação de diversos cargos pelas PcD. No entanto, a baixa qualificação desse público ainda é a maior dificuldade para sua contratação. Esse artigo relata a experiência de uma instituição na capacitação de PcD em temas de Tecnologia da Informação e Comunicação. Diferentemente de outras iniciativas focadas apenas no ensino de Informática Básica, o projeto aqui descrito prepara as PcD para atuarem no desenvolvimento ou teste de software. O artigo mostra os processos de concepção e condução do projeto, suas inovações constantes, bem como os resultados que proporcionam às PcD melhores condições de trabalho.
Meio ambiente e sustentabilidade
Authors: Silva Aline Marcelino dos Santos , Moraes Deiz Amara Silva de Souza , Batista Silvia Cristina Freitas
Published: January 2022

Este artigo descreve dois momentos de uma pesquisa realizada no Ensino Fundamental com o uso do Scratch sobre a temática Meio Ambiente e Sustentabilidade. No primeiro momento, um estudo de caso foi realizado com estudantes do primeiro segmento do Ensino Fundamental. Nessa etapa, três objetos de aprendizagem (OA) foram elaborados pelas autoras deste artigo e, posteriormente, utilizados no estudo de caso. Ainda nessa primeira fase, os OA foram avaliados por cinco professores, em relação a questões operacionais, de usabilidade, pedagógicas e de conteúdo. No segundo momento, estudantes do segundo segmento do Ensino Fundamental produziram histórias no Scratch. produzidos foram importantes para a identificação de aspectos a serem observados no desenvolvimento de recursos.
Rotation and transposition flaps in facial plastic and reconstructive surgery
Authors: Sciegienka Sebastian , Hanick Andrea , Branham Gregory
Published: January 2022

Reconstructive defects of the human face pose unique challenges to even the most experienced surgeon given their myriad of presentations and the individuality of each patient’s anatomy, clinical presentation, and perspective or preferences. A robust armamentarium of reconstructive options must be cultivated for each facial subunit so that experience and artistry can be best utilized to rebuild the patient’s structure and function. This review will outline a subset of local rotation and transposition flaps that are most useful for facial reconstruction, organized by facial subunit.
Histological analysis of thrombi retrieved after acute ischemic stroke from large vessel occlusion: from research to clinical practice
Authors: Nicolini Ettore , De Michele Manuela , Falcou Anne , Petraglia Luca , Berto Irene , Toni Danilo
Published: January 2022

Emergent reperfusion therapies have improved acute ischemic stroke prognosis, but many patients are still bound to bad clinical outcome, probably because of our incomplete knowledge of its pathophysiology. Thanks to mechanical thrombectomy, occluding material is available for histological analysis. Several studies investigated the possible relationship between thrombus composition and clinical, procedural, and radiological variables of acute ischemic stroke. The potential value of thrombus analysis as a tool for clinical practice and research is still not defined, as data from the literature are heterogeneous and sometimes conflicting. We propose a review of the existing literature regarding histological analysis of thrombi in acute ischemic stroke. We classified articles on clot composition according to the clinical variable explored in each study. We first distinguished articles about etiology, procedural, and radiological variables, and then we performed a subclassification for each group. This review could help both in the interpretation of thrombus analysis in clinical practice and in its usage for future research.
Percutaneous catheter-based repeat revascularization in patients with previous PCI or CABG: a comprehensive review of the evidence
Authors: Valooran George Jose , Subbiah Meenakshi , Idhrees Mohammed , Karuppannan Mukesh , Bashir Mohamad , Velayudhan Bashi
Published: January 2022

Repeat revascularization after percutaneous coronary intervention (PCI) or coronary artery bypass grafting (CABG) is one of the most common long-term complications which warrants continuous clinical follow up. Re-interventions negatively impact long-term survival in patients with coronary artery disease. The repeat revascularization after PCI can be either a target lesion revascularization (stent thrombosis/in-stent restenosis) or a revascularization of native coronary artery after PCI (target vessel revascularization/non-target vessel revascularization). The EVENT registry reports that repeat revascularization rates in patients undergoing PCI is 12% in the first year of follow up. Repeat revascularization with additional stent deployment increases the rate of stent thrombosis and restenosis, thereby leading to recurrent ischemic events. Repeat revascularization after CABG can be either in the early postoperative period or later due to native disease progression or late graft stenosis. The need for re-intervention after surgical or percutaneous revascularization is inevitable and is dependent on modifiable and non-modifiable risk factors.
Referring hospital involvement in early discharge post transcatheter aortic valve implantation: the TAVI (R-) EXPRES program
Authors: van Wiechen Maarten P. , de Ronde-Tillmans Marjo J. , Van Mieghem Nicolas M.
Published: January 2022

Aim: Over the past decade, transcatheter aortic valve implantation (TAVI) has matured into a valid treatment strategy for elderly patients with severe aortic stenosis. TAVI programs will grow with its adoption in low-risk patients. The aim of this study was to evaluate safety and feasibility of early discharge protocols, either home or back to a referring hospital. Methods: Consecutive patients undergoing TAVI between July 2017 and July 2019 were stratified into three discharge pathways from TAVI center: (1) early home (EXPRES); (2) early transfer to referring hospital (R-EXPRES); and (3) routine discharge (standard). Baseline, procedural, and 30-day outcomes were prospectively collected and compared per discharge pathway. Results: In total, 22 (5%) patients were enrolled in the EXPRES cohort [median age 78 (IQR: 73-81); mean Society of Thoracic Surgeons (STS) 2.4% ± 1.5%], 121 (29%) in the R-EXPRES cohort [median age 81 (IQR: 77-84); mean STS 4.3% ± 2.8%], and 269 (65%) in the routine discharge cohort [median age 80 (IQR: 75-85); mean STS 4.4% ± 3.1%]. EXPRES patients trended to be younger (P = 0.13) and had lower STS (P = 0.02). Early clinical outcome was similar through the different pathways including re-hospitalization rate. Median length of stay was one day longer for R-EXPRES vs. routine discharge patients [5 (IQR: 4-7) vs. 4 (IQR: 3-6); P < 0.01]. Median length of stay (LOS) was two days (IQR: 1-3 days) for EXPRES patients. Conclusion: Early discharge pathways home and to referral hospitals are safe and help streamline TAVI programs. LOS in referring hospitals may be further reduced.
Management of concomitant coronary artery disease and aortic valve stenosis in the era of transcatheter aortic valve treatment
Authors: Cangemi Stefano , Aurigemma Cristina , Romagnoli Enrico , Bianchini Francesco , Bruno Piergiorgio , Nesta Marialisa , Burzotta Francesco , Trani Carlo
Published: January 2022

Severe calcific aortic stenosis (AS) and coronary artery disease (CAD) have common risk factors and are frequently encountered in the same patient in clinical practice. CAD has been reported in ≥ 50% of AS patients undergoing both surgical treatment and transcatheter aortic valve implantation (TAVI). In the last two decades, TAVI has been established as a less invasive alternative to surgery. Recently, more and more young and low surgical risk patients undergo TAVI. Despite the high prevalence of CAD in patients treated with TAVI, the management strategy of concomitant CAD in these patients remains an area of considerable uncertainty. This review provides an updated overview of the current knowledge about this topic and offers points for reflection about the best approach to use.
Emerging actionable targets to treat therapy-resistant colorectal cancers
Authors: Grassilli Emanuela , Cerrito Maria Grazia
Published: January 2022

In the last two decades major improvements have been reached in the early diagnosis of colorectal cancer (CRC) and, besides chemotherapy, an ampler choice of therapeutic approaches is now available, including targeted and immunotherapy. Despite that, CRC remains a “big killer” mainly due to the development of resistance to therapies, especially when the disease is diagnosed after it is already metastatic. At the same time, our knowledge of the mechanisms underlying resistance has been rapidly expanding which allows the development of novel therapeutic options in order to overcome it. As far as resistance to chemotherapy is concerned, several contributors have been identified such as: intake/efflux systems upregulation; alterations in the DNA damage response, due to defect in the DNA checkpoint and repair systems; dysregulation of the expression of apoptotic/anti-apoptotic members of the BCL2 family; overexpression of oncogenic kinases; the presence of cancer stem cells; and the composition of the tumoral microenvironment and that of the gut microbiota. Interestingly, several mechanisms are also involved in the resistance to targeted and/or immunotherapy. For example, overexpression and/or hyperactivation and/or amplification of oncogenic kinases can sustain resistance to targeted therapy whereas the composition of the gut microbiota, as well as that of the tumoral niche, and defects in DNA repair systems are crucial for determining the response to immunotherapy. In this review we will make an overview of the main resistance mechanisms identified so far and of the new therapeutic approaches to overcome it.
Colon cancer in a 12-year-old girl with hypertriglyceridemia
Authors: Pedroni Monica , Leon Maurizio Ponz de , Bonetti Luca Reggiani , Viel Alessandra , Noto Davide , Nascimbeni Fabio , Sena Paola , Roncucci Luca
Published: January 2022

Colorectal cancer is usually considered a disease of the elderly; however, in a small fraction of patients (2%-3% of all affected individuals), colorectal malignancies may develop earlier. The reasons whereby some individuals develop colorectal cancer at a young age are poorly understood. In a 12-year-old girl, a malignancy was diagnosed in the ascending colon. There was no familial history of Lynch syndrome or familial adenomatous polyposis. The metabolic profile of the patient revealed hypertriglyceridemia and low high-density lipoprotein cholesterol levels at nine years, then diagnosed as familial hypertriglyceridemia due to a constitutional mutation in the APOA5 gene (c.427delC). Moreover, variants possibly increasing the risk of cancer were detected in MSH6 (c.3438+11_3438+14delCTTA, intron 5) and APC (I1307K). The patient showed a rather unusual dietary pattern, since her basic alimentation from weaning consisted almost exclusively of meat homogenates and, subsequently, roasted meat or cutlets. Other foods, including fish, vegetables, sweets, and pasta, were refused. In this case, genetic and environmental factors could have acted in a particularly accelerated manner. Indeed, the genetic background of the patient (familial hypertriglyceridemia and polymorphisms predisposing to colorectal cancer) may have favored a dietary-driven colorectal carcinogenesis, resulting in an extremely early onset development of malignancy.
Adherence to lifestyle modification in patients with nonalcoholic fatty liver disease
Authors: Andreev K. A. , Skirdenko Yu. P. , Nikolaev N. A. , Livzan M. A. , Gorbenko A. V. , Fedorin M. M. , Krolevets T. S.
Published: January 2022

Nonalcoholic fatty liver disease (NAFLD) makes a major impact on morbidity and mortality among the workingage population in developed countries. In the lack of effective pharmacological methods, the leading role in treatment of NAFLD belongs to lifestyle modification, consistent and gradual weight loss, and its maintenance. The qualitative and quantitative structure of the diet, intensity of physical activity, and most importantly, regularity and consistency of implementation of lifestyle modification activities are the key to successful management of patients with NAFLD.

To date, there are very few studies on adherence to lifestyle modification activities in this group of patients, which is mainly due to a deficiency of methodological tools. The questionnaire “QAA-25” recommended by the Russian Scientific Medical Society of Therapists for quantitative assessment of adherence to treatment allows to assess both adherence to therapy in general and adherence to its individual components (adherence to drug therapy, adherence to medical counseling, and adherence to lifestyle modification), which requires further study taking into account features of therapeutic strategies in treating NAFLD.
Clinical and morphological phenotypes in intrathoracic sarcoidosis
Authors: Palchikova I. A. , Denisova O. A. , Chernyavskaya G. M. , Purlik I. L. , Kalacheva T. P. , Naumov A. O. , Soloviev M. M.
Published: January 2022

Aim. To study clinical and morphological phenotypes in different variants of the course of intrathoracic sarcoidosis and isolate new phenotypes.

Materials and methods. The study included 121 patients with intrathoracic sarcoidosis aged 21–66 years (50.4% were men, 49.6% were women, the average age at the time of the disease onset was 38 years) over the period 2007– 2019. During the examination, patients’ complaints were studied thoroughly, and the diagnosis was histologically verified in all cases. During an extended histological examination, the quantitative and qualitative composition of biopsy specimens was investigated. The number of granulomas in the field of vision and the content of giant cells, macrophages, lymphocytes, neutrophils, and eosinophils in them were studied. Qualitative parameters were assessed for the presence of hyalinosis, Schaumann bodies, necrosis, stamping, calcification, fibrosis, and vasculitis. All patients were retrospectively divided into two clinical groups depending on the outcomes of the disease: group 1 included patients with a favorable course of sarcoidosis, proceeding without relapses and signs of progression; group 2 encompassed patients with an unfavorable course of the disease with relapses and progression, requiring long-term administration of systemic glucocorticoids.

Results. The analysis showed that among all general clinical manifestations, only the presence of dyspnea, skin manifestations, and weight loss occurred significantly more often in the patients with an unfavorable course of intrathoracic sarcoidosis (р = 0.04; 0.02; and 0.01, respectively). Among morphological parameters, a large number of macrophages was significantly more frequent in the biopsy specimens in this group of patients (р < 0.01).
Work of a stroke team: experience of transferring ischemic stroke patients from district hospitals to Krasnoyarsk Regional Vascular Center for thrombectomy
Authors: Shniakin P. G. , Isaeva N. V. , Kuznetsov V. Y. , Protopopov A. V. , Korchagin E. E. , Dovbish N. Y. , Litvinyuk N. V.
Published: January 2022

Modern high-technology methods for ischemic stroke treatment (systemic thrombolysis, mechanical thrombectomy, thrombaspiration, stenting of cerebral arteries) can improve the rehabilitation potential and survival of patients. Important tasks here are selection for reperfusion and its performance on the greatest possible number of peracute patients. Mechanical thrombectomy combined with systemic thrombolysis is the most effective reperfusion strategy in the therapeutic window, but the availability of endovascular methods is limited to highly specialized centres. One way to solve this problem is to organize effective logistics with stroke patients, which will provide high-tech care for patients living far from large treatment centers due to regulated interaction between institutions at different levels.

The aim of the study was to improve emergency interaction related to transfer of peracute stroke patients from primary vascular units and district hospitals of the Krasnoyarsk region to Krasnoyarsk Regional Vascular Center for thrombectomy.
Characteristics of lipid peroxidation processes and factors of the antioxidant defense system in chronic atrophic gastritis and gastric cancer
Authors: Smirnova O. V. , Tsukanov V. V. , Sinyakov A. A. , Moskalenko O. L. , Elmanova N. G. , Ovcharenko E. S. , Kasparov E. V.
Published: January 2022

Background. The problem of gastric cancer remains unresolved throughout the world, while chronic atrophic gastritis (CAG) increases the likelihood of its development by 15 times. In the Russian Federation, the incidence of gastric cancer (GC) is among the highest, with it prevailing among males. One of the leading mechanisms in molecular pathology of membranes is lipid peroxidation (LPO). The severity of oxidative membrane damage depends on concomitant diseases, contributing to emergence and progression of pathological processes and development of cancer. Currently, the problem of LPO is unsolved in biological systems.

The aim of this study was to investigate the state of LPO and antioxidant defense system in CAG and GC. Materials and methods. The parameters were studied in 45 patients with CAG and 50 patients with GC. The control group included 50 practically healthy volunteers without gastrointestinal complaints, who did not have changes in the gastric mucosa according to the fibroesophagogastroduodenoscopy (FEGDS) findings.

Results. In patients with CAG, an increase in malondialdehyde, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase was found in the blood plasma compared with the control group. In patients with CAG, lipid peroxidation was activated, and the malondialdehyde level increased by 3.5 times relative to normal values. At the same time, the body fought against oxidative stress by increasing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase. All patients with GC showed pronounced oxidative stress in the blood plasma in the form of a 45-fold increase in malondialdehyde. The activity of the main antioxidant enzyme superoxide dismutase was reduced in GC. Catalase was activated, which indicated pronounced oxidative stress, significant damage to blood vessels, and massive cell death. Glutathione-related enzymes (glutathione S-transferase and glutathione peroxidase) and the antioxidant protein ceruloplasmin were activated, which also indicated significant oxidative stress and severe intoxication in patients with GC.

Conclusion. Depending on the stage and type of cancer, an in-depth study of lipid peroxidation and factors of the antioxidant defense system can be used to correct therapy and prevent cancer and can serve as markers of progression and prognosis in gastric cancer.
Application of the Autoregressive Integrated Moving Average Exogenous (ARIMAX) with Calendar Variation Effect Method for Forecasting Chocolate Data in Indonesia and the United States
Authors: Syam Andy Rezky Pratama
Published: January 2022

Forecasting chocolate consumption is required by producers in preparing the amount of production each month. The tradition of Valentine, Christmas and Eid al-Fitr which are closely related to chocolate makes it impossible to predict chocolate by using the Classical Time Series method. Especially for Eid al-Fitr, the determination follows the Hijri calendar and each year advances 10 days on the Masehi calendar, so that every three years Eid al-Fitr will occur in a different month. Based on this, the chocolate forecasting will show a variation calendar effect. The method used in modeling and forecasting chocolate in Indonesia and the United States is the ARIMAX (Autoregressive Integrated Moving Average Exogenous) method with Calendar Variation effect. As a comparison, modeling and forecasting are also carried out using the Naïve Trend Linear, Naïve Trend Exponential, Double Exponential Smoothing, Time Series Regression, and ARIMA methods. The ARIMAX method with Calendar Variation Effect produces a very precise MAPE value in predicting chocolate data in Indonesia and the United States. The resulting MAPE value is below 10 percent, so it can be concluded that this method has a very good ability in forecasting.
Optimization of Raw Material Inventory Control CV. Dirga Eggtray Pinrang Using Probabilistic Model with Backorder and Lostsales Condition
Authors: Resky Aprizal , Rangkuti Aidawayati , Tinungki Georgina M.
Published: January 2022

This research discusses about the comparison of raw material inventory control CV. Dirga Eggtray Pinrang. It starts with forecasting inventory for the next 12 periods using variations of the time series forecasting method, where the linear regression method provides accurate forecasting results with a Mean Absolute Percentage Error (MAPE) value of 1,9371%. The probabilistic models of inventory control used are the simple probabilistic model, Continuous Review System (CRS) model, and Periodic Review System (PRS) model. The CRS model with backorder condition is a model that provides the minimum cost of Rp. 969.273.706,20 per year compared to another probabilistic model with the largest difference of Rp. 1.291.814,95 per year, with the optimum number of order kg, reorder level kg, and safety stock kg.
ANALYSIS OF SYNTACTIC FUNCTIONS AND SYNTACTIC CATEGORIES ON KITAB TA’LIM MUTA’ALIM
Authors: Nurazmi Isti Azhura , Muhammad Zaka Al Farisi  , Syihabuddin
Published: January 2022

The purpose of this research is getting discription explicity about : syntatic functions and syntatic categories in Kitab Ta’lim Muta’alim. This Research is descriptive research with a comparative-contransitive approach with using special tables of classificate output of research data.

               Based on data analysis of research done by research. The conclusion of the research is that there are 35 speech acts of directive commands in the Ta'lim Mutaalim translation book in which syntactic functions and syntactic categories are written. the translation results include the ideology of foreignization.
THE COMPARISON OF PROPOLIS EFFECTS ON TUMOR NECROSIS FACTOR ALPHA AND MALONDIALDEHYDE BETWEEN INHALATION AND CUTANEOUS ANTHRAX ANIMAL MODELS
Authors: Redhono Harioputro Dhani , Sanjaya Wisnu , Werdiningsih Yulyani
Published: January 2022

Background: Inflammatory response and oxidative stress can be found in anthrax characterized by increased level of serum Tumor Necrosis Factor Alpha (TNF-α) and Malondialdehyde (MDA). The use of antibiotics in anthrax has been known to cause some disturbing side-effects, such as allergic reaction, nausea, vomiting, and antibiotic resistance. Thus, ethanolic extract of propolis (EEP) might be the alternative regimen, due to its anti-inflammatory and antioxidant properties. This study aimed to compare the effects of ethanolic extract of propolis (EEP) on TNF-α and MDA between the inhalation and cutaneous anthrax animal model. Materials and Methods: This was an experimental study with a post-test-only control group design on 40 samples of Rattus norvegicus. Samples were randomized into 5 groups: control, inhalation anthrax model, inhalation anthrax model + EEP, cutaneous anthrax model, and cutaneous anthrax model + EEP. After 14 days, the level of TNF-α and MDA were measured. To compare the data, we used the ANOVA test continued by the post-hoc Turkey test. Results: The results obtained showed that the level of TNF-α and MDA between the inhalation and cutaneous anthrax animal models treated with EEP were statistically different (p < 0.05). The P5 group showed the lowest level of TNF-α (6.822 ± 0.383 pg/ml) and MDA (2.717 ± 0.383 nmol/ml). Conclusion: EEP has a better effect on reducing TNF-α and MDA in cutaneous anthrax animal models compared to the inhalation anthrax animal model.