bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the impact of land use and changes in land cover related to carbon storage by linking trajectory analysis and InVEST models in the Nandu River Basin on Hainan Island in China
Authors: Gong Wenfeng , Duan Xuanyu , Mao Mingjiang , Hu Jihan , Sun Yuxin , Wu Genghong , Zhang Yangyang , Xie Yidan , Qiu Xincai , Rao Xiaodong , Liu Tiedong , Liu Tao
Published: October 2022

This study aims to evaluate the effects of the spatiotemporal patterns of land-use and land-cover (LULC) changes on the dynamics of carbon storage in a tropical region of China by linking the trajectory analysis of LULC changes and the InVEST model. Based on remote sensing (RS), geographic information system (GIS) and change trajectories, the spatiotemporal evolution of LULC changes was explored. This evolution could be coupled with the spatiotemporal LULC change trajectories and the InVEST model for the quantitative study of the spatial distribution and temporal variation in regional carbon stocks. The results showed that during the 2000–2020 period, the built-up land continually increased to 206.05 km2 through urban expansion, and forestland became the dominant type of land, with an area of 357.39 km2. In addition to the change in land use, the carbon storage in the study region increased by 4.87 Tg C. The anaphasic trajectory had the largest area ratio at 7.05% in the total area, while the prophasic trajectory contributed to the largest increase in carbon storage, 5.87 Tg C. Moreover, the repetitive trajectories had no impact on carbon sinks and sources, whereas the anaphasic trajectory and the continual trajectory imposed passive impacts on carbon storage. These advances in research underpin scientific efforts to improve the understanding of the relationship between the optimization of land-use structure and patterns and the carbon storage service in the Nandu River Basin.
Lateral stress and its transmission law caused by operation of a double-wing subsoiler in sandy loam soil
Authors: Qin Kuan , Zhao Yun , Zhang Yongzheng , Cao Chengmao , Shen Zhougao
Published: October 2022

Sandy loam soils are widespread and important for agricultural soil use. The lateral stress caused by the loosening shovel of a subsoiler produces lateral disturbances in sandy loam soil, reducing compaction and improving soil structure. To explore the variation and transmission of lateral stress due to the operation of a double-wing subsoiler in sandy loam soil, a lattice of point-type soil sensors was arranged in a soil bin, and the lateral stress generated by subsoiling was measured in the shallow, medium, and deep soil layers. The experimental results show that when the lateral stress generated by the double-wing subsoiler is transferred to the lateral position of different distances.The lateral stress shows a sinusoidal fluctuation law with time, the sine wave frequency range is: shallow (0.366–0.549); middle (0.306–0.494); and deep (0.088–0.501). There were greater fluctuation amplitudes in the transfer process for the shallow and deep soil layers than for the middle layer. Shallow soil had the largest final stable lateral stress (0.1617 N), and middle soil the smallest (0.0733 N). Thus, lateral stress from deep loosening has the greatest effect on shallow soil and the least effect on middle soil. These results indicate that the fluctuating lateral stresses generated by double-wing-subsoiler operation drive a fluctuating soil disturbance that can improve soil structure, and that they are greater for shallow and deep soils than for middle soils.
Does environmentally biased technological progress reduce air pollution emissions? empirical analysis based on spatial model and PSTR model
Authors: Meng Yuxin , Liu Lu , Ran Qiying
Published: October 2022

Environmental policies and Environmentally biased technological advances have important theoretical value and practical significance for air pollution reduction and green economic development in China. The article is based on panel data for 270 cities in China from 2007 to 2020. Using a Spatial model, the direct, spillover and moderating effects of Environmentally biased technological progress on “local-neighbourhood” urban air pollution are examined empirically. On this basis, the PSTR model is used to further explore the non-linear effects of different types of environmental regulations. The study found that: 1) There is a significant positive spatial correlation between both air pollution and Environmentally biased technological progress in China. Environmentally biased technological progress significantly suppresses air pollution, with significant spatial spillover effects, and the conclusions remain robust after a series of robustness tests. 2) Different types of environmental regulations reinforce the dampening effect of Environmentally biased technological progress on air pollution. However, informal environmental regulation only reinforces the dampening effect of Environmentally biased technological progress on air pollution in the local cities. 3) There is a significant threshold effect of environmental regulation on the impact of Environmentally biased technological progress on air pollution. When the level of environmental regulation within a certain threshold interval, Environmentally biased technological progress has a dampening effect on air pollution. 4) The more eastern the city, the larger the city and the non-resource-based cities, the more significant the air pollution suppression effect of Environmentally biased technological advances. Based on this, this study points out that governments at all levels should pay attention to the spatial spillover effect of technological progress bias on air pollution, deepen the construction of joint prevention and control mechanisms for regional air pollution control; Establish multiple environmental regulation policies, formulate and implement environmental regulation policies according to local conditions and local technological progress bias; Formulate targeted environmental regulation policies and technological innovation policies according to the characteristics of different cities. It is an important theoretical reference to help China to fight the battle against air pollution in-depth and to promote further improvement of ecological and environmental quality.
Functional traits above and below ground allow species with distinct ecological strategies to coexist in the largest seasonally dry tropical forest in the Americas
Authors: Fagundes Marina Vergara , Souza Alexandre F. , Oliveira Rafael S. , Ganade Gislene
Published: October 2022

Plant functional strategies are well-established for low- and high-stress environments, such as rainforests and deserts. However, in environments with low- and high-stress level fluctuation within years, the relationship between plant functional strategies and their spatial distribution is still poorly understood. We aimed to answer: what are the relationships between above- and below-ground traits in the largest seasonally dry tropical forest in the Americas? Do the studied species form detectable groups from the functional perspective? If detectable, do functional groups present distinct spatial distributions across the domain, mediated by spatial heterogeneity of aridity? We sampled a range of 16 above- and below-ground traits from the 20 most common native tree species. We performed a PCA to understand the species' main coordinated trade-offs, a k-mean analysis to test for functional groups, and a Ripley's-K analysis followed by a GLS model to test spatial functional groups distribution through the aridity gradient. We found five coordinated trade-offs representing different aspects of the conservative-acquisitive strategy continuum. Drought-tolerance and avoidance mechanisms seem linked to the conservative-acquisitive gradient, where water storage is positively correlated with acquisitive strategies. Different from other seasonally dry regions, acquisitive strategies are not limited by aridity. The presence of short-term water storage traits might buffer rainfall fluctuations, allowing acquisitive species to occupy more arid regions. This study sheds new light on the functional complexity of species from Americas seasonally dry tropical forests, for the first time including the relationship of its below- and above-ground traits.
A comprehensive analysis of metabolomics and transcriptomics to reveal major metabolic pathways and potential biomarkers of human preeclampsia placenta
Authors: Feng Yan , Lian Xinlei , Guo Kaimin , Zhang Guanglan , Huang Xuan
Published: October 2022

Background: The etiology of preeclampsia (PE) remains unclear. With the utilization of metabolomics, dysregulated production of several metabolic components in human plasma, such as lipids, amino acids, androgens and estrogens, was found to be important in the pathogenesis of PE. Transcriptomics adds more in-depth information, and the integration of transcriptomics and metabolomics may yield further insight into PE pathogenesis than either one alone.

Objectives: We investigated the placental metabolomics and transcriptomics of PE patients to identify affected metabolic pathways and potential biological targets for exploring the disease pathogenesis.

Methods: Integrated transcriptomics and metabolomics were used to analyze five paired human placentas from patients with severe PE and normal pregnancies. This was followed by further validation of our findings in a publicly available dataset of 173 PE vs. 157 control placentas. In addition, weighted gene coexpression network construction was performed to assess the correlation between genetic alterations and diseases.

Results: We identified 66 and 41 differentially altered metabolites in negative and positive ion modes, respectively, in the PE group compared to the control group, and found 2,560 differentially expressed genes. Several pathways were aberrantly altered in the PE placenta at both the metabolic and transcriptional levels, including steroid hormone biosynthesis, the cAMP signaling pathway, neuroactive ligand–receptor interactions, taste transduction and prion diseases. Additionally, we found 11 differential metabolites and 11 differentially expressed genes involved in the steroid hormone biosynthesis pathway, indicating impaired metabolism of steroid hormones in the PE placenta. Furthermore, we found that CYP11A1, HSD3B2, and HSD17B6 are highly correlated with diseases.

Conclusion: Our findings provide a profile of the dysregulated steroid hormone biosynthesis in PE placenta, we observed a dysregulated cortisol-to-cortisone ratio, testosterone accumulation, decreased testosterone downstream metabolites, impaired production of estrone and estriol, and aberrant hydroxylation and methylation of estradiol. Disorders of placental steroid hormone metabolism might be a consequence or a compensatory change in pathological placentation in PE, which underscores the need to investigate the physiology of steroid hormone metabolites in the etiology of PE.
Engineered red blood cells (activating antigen carriers) drive potent T cell responses and tumor regression in mice
Authors: Blagovic Katarina , Smith Carolyne K. , Ramakrishnan Amritha , Moore Lindsay , Soto David R. , Thompson Zachary , Stockmann Adam P. , Kruszelnicki Sonia , Thakkar Akshi , Murray Jason , Torres Sebastian , Wondimagegnhu Bersabel , Yi Roslyn , Dadgar Maisam , Paracha Abdul M. , Page Claire , Clear Louise , Chaudhry Omer A. , Myint Melissa , Bridgen Devin T. , Gilbert Jonathan B. , Seidl Katherine J. , Sharei Armon , Loughhead Scott , Bernstein Howard , Yarar Defne
Published: October 2022

Activation of T cell responses is essential for effective tumor clearance; however, inducing targeted, potent antigen presentation to stimulate T cell responses remains challenging. We generated Activating Antigen Carriers (AACs) by engineering red blood cells (RBCs) to encapsulate relevant tumor antigens and the adjuvant polyinosinic-polycytidylic acid (poly I:C), for use as a tumor-specific cancer vaccine. The processing method and conditions used to create the AACs promote phosphatidylserine exposure on RBCs and thus harness the natural process of aged RBC clearance to enable targeting of the AACs to endogenous professional antigen presenting cells (APCs) without the use of chemicals or viral vectors. AAC uptake, antigen processing, and presentation by APCs drive antigen-specific activation of T cells, both in mouse in vivo and human in vitro systems, promoting polyfunctionality of CD8+ T cells and, in a tumor model, driving high levels of antigen-specific CD8+ T cell infiltration and tumor killing. The efficacy of AAC therapy was further enhanced by combination with the chemotherapeutic agent Cisplatin. In summary, these findings support AACs as a potential vector-free immunotherapy strategy to enable potent antigen presentation and T cell stimulation by endogenous APCs with broad therapeutic potential.
SARS-CoV-2-Specific IgG and IgA response in maternal blood and breastmilk of vaccinated naïve and convalescent lactating participants
Authors: Longueira Yesica , Ojeda Diego S. , Battistelli Rocio B. Antivero , Sanchez Lautaro , Oviedo Rouco Santiago , Albano Daniel , Guevara Eleonora , Valls Vanesa , Pando María A. , Gamarnik Andrea V.
Published: October 2022

Background

Recent studies have shown the presence of SARS-CoV-2-specific antibodies in the milk of breastfeeding mothers vaccinated with mRNA and convalescent. However, limited information is available in lactating women receiving other vaccine platforms used in developing countries, such as the inactivated SARS-CoV-2 vaccine BBIBP-CorV (Sinopharm) and the non-replicating adenovirus vaccines Sputnik V (Gamaleya Institute) and ChAdOx1-S (Oxford AstraZeneca).

Methods

Here, we evaluated anti-SARS-CoV-2 IgG and IgA levels in both serum and milk samples using a longitudinal and a cross-sectional cohort of 208 breastfeeding vaccinated women from Argentina with or without previous SARS-CoV-2 infection.

Results

The analysis showed that IgA levels remain constant in serum and milk of breastfeeding mothers between the first and second doses of vector-based vaccines (Sputnik V and ChAdOx1-S). After the second dose, anti-spike IgA was found positive in 100% of the serum samples and in 66% of breastmilk samples. In addition, no significant differences in milk IgA levels were observed in participants receiving BBIBP-CorV, Sputnik V or ChAdOx1-S. IgG levels in milk increased after the second dose of vector-based vaccines. Paired longitudinal samples taken at 45 and 120 days after the second dose showed a decrease in milk IgG levels over time. Study of IgA levels in serum and milk of vaccinated naïve of infection and vaccinated-convalescent breastfeeding participants showed significantly higher levels in vaccinated-convalescent than in participants without previous infection.

Conclusion

This study is relevant to understand the protection against SARS-CoV-2 by passive immunity in newborns and children who are not yet eligible to receive vaccination.
Biomarkers and mechanisms of tolerance induction in food allergic patients drive new therapeutic approaches
Authors: Baloh Carolyn H. , Huffaker Michelle F. , Laidlaw Tanya
Published: October 2022

Immunotherapy for food-allergic patients has been effective in inducing desensitization in some populations, but long-term tolerance has remained an elusive target. A challenge facing our field is how to differentiate immune markers that are impacted by immunotherapy from those that are critical biomarkers of tolerance. Data from recent clinical trials have identified several biomarkers and mechanisms for achieving tolerance. These biomarkers include younger age, lower food-specific IgE, lower food component-specific IgE, specific linear epitope profiles, and subsets of food-specific CD4+ T cells. Additional biomarkers under investigation for their relevance in tolerance induction include TCR repertoires, gastrointestinal and skin microbiome, and local tissue immunity. This mini-review highlights recent advances in understanding biomarkers and mechanisms of tolerance induction in food immunotherapy and how these are influencing clinical trial development.
Emerging roles for IL-25 and IL-33 in colorectal cancer tumorigenesis
Authors: Jou Eric , Rodriguez-Rodriguez Noe , McKenzie Andrew N. J.
Published: October 2022

Colorectal cancer (CRC) is the second leading cause of cancer-related death worldwide, and is largely refractory to current immunotherapeutic interventions. The lack of efficacy of existing cancer immunotherapies in CRC reflects the complex nature of the unique intestinal immune environment, which serves to maintain barrier integrity against pathogens and harmful environmental stimuli while sustaining host-microbe symbiosis during homeostasis. With their expression by barrier epithelial cells, the cytokines interleukin-25 (IL-25) and IL-33 play key roles in intestinal immune responses, and have been associated with inappropriate allergic reactions, autoimmune diseases and cancer pathology. Studies in the past decade have begun to uncover the important roles of IL-25 and IL-33 in shaping the CRC tumour immune microenvironment, where they may promote or inhibit tumorigenesis depending on the specific CRC subtype. Notably, both IL-25 and IL-33 have been shown to act on group 2 innate lymphoid cells (ILC2s), but can also stimulate an array of other innate and adaptive immune cell types. Though sometimes their functions can overlap they can also produce distinct phenotypes dependent on the differential distribution of their receptor expression. Furthermore, both IL-25 and IL-33 modulate pathways previously known to contribute to CRC tumorigenesis, including angiogenesis, tumour stemness, invasion and metastasis. Here, we review our current understanding of IL-25 and IL-33 in CRC tumorigenesis, with specific focus on dissecting their individual function in the context of distinct subtypes of CRC, and the potential prospects for targeting these pathways in CRC immunotherapy.
Dermal fibroblasts are the key sensors of aseptic skin inflammation through interleukin 1 release by lesioned keratinocytes
Authors: Cordier-Dirikoc Sevda , Pedretti Nathalie , Garnier Julien , Clarhaut-Charreau Sandrine , Ryffel Bernhard , Morel Franck , Bernard François-Xavier , Hamon de Almeida Valérie , Lecron Jean-Claude , Jégou Jean-François
Published: October 2022

IL-1 plays a crucial role in triggering sterile inflammation following tissue injury. Although most studies associate IL-1 release by injured cells to the recruitment of neutrophils for tissue repair, the inflammatory cascade involves several molecular and cellular actors whose role remains to be specified. In the present study, we identified dermal fibroblasts among the IL-1R1-expressing skin cells as key sensors of IL-1 released by injured keratinocytes. After in vitro stimulation by recombinant cytokines or protein extracts of lysed keratinocytes containing high concentrations of IL-1, we show that dermal fibroblasts are by far the most IL-1-responsive cells compared to keratinocytes, melanocytes and endothelial cells. Fibroblasts have the property to respond to very low concentrations of IL-1 (from 10 fg/ml), even in the presence of 100-fold higher concentrations of IL-1RA, by increasing their expression of chemokines such as IL-8 for neutrophil recruitment. The capacity of IL-1-stimulated fibroblasts to attract neutrophils has been demonstrated both in vitro using cell migration assay and in vivo using a model of superficial epidermal lesion in IL-1R1-deficient mice which harbored reduced expression of inflammatory mediators and neutrophil skin infiltration. Together, our results shed a light on dermal fibroblasts as key relay cells in the chain of sterile inflammation induced after epidermal lesion.
MIS-C: A COVID-19-as sociated condition between hypoimmunity and hyperimmunity
Authors: Gelzo Monica , Castaldo Alice , Giannattasio Antonietta , Scalia Giulia , Raia Maddalena , Esposito Maria Valeria , Maglione Marco , Muzzica Stefania , D’Anna Carolina , Grieco Michela , Tipo Vincenzo , La Cava Antonio , Castaldo Giuseppe
Published: October 2022

Multisystem inflammatory syndrome in children (MIS-C) is a rare, severe complication of COVID-19. A better knowledge of immunological, cellular, and genetic characteristics of MIS-C could help better understand the pathogenesis of the disease and contribute to identifying specific diagnostic biomarkers and develop targeted therapies. We studied 37 MIS-C children at hospital admission and 24 healthy controls analyzing serum cytokines (IFN-α, IFN-β, IFN-γ, IL-6, IL-10, IL-17A, IL-12p70 and TNF), lymphocyte populations by flow cytometry and 386 genes related to autoimmune diseases, autoinflammation and primary immunodeficiencies by NGS. MIS-C patients showed a significant increase of serum IFNγ (despite a significant reduction of activated Th1) and ILs, even if with a great heterogeneity among patients, revealing different pathways involved in MIS-C pathogenesis and suggesting that serum cytokines at admission may help to select the inflammatory pathways to target in each patient. Flow cytometry demonstrated a relevant reduction of T populations while the percentage of B cell was increased in agreement with an autoimmune pathogenesis of MIS-C. Genetic analysis identified variants in 34 genes and 83.3% of patients had at least one gene variant. Among these, 9 were mutated in more patients. Most genes are related to autoimmune diseases like ATM, NCF1, MCM4, FCN3, and DOCK8 or to autoinflammatory diseases associated to the release of IFNγ like PRF1, NOD2, and MEF. Thus, an incomplete clearance of the Sars-CoV2 during the acute phase may induce tissue damage and self-antigen exposure and genetic variants can predispose to hyper-reactive immune dysregulation events of MIS-C-syndrome. Type II IFN activation and cytokine responses (mainly IL-6 and IL-10) may cause a cytokine storm in some patients with a more severe acute phase of the disease, lymphopenia and multisystemic organ involvement. The timely identification of such patients with an immunocytometric panel might be critical for targeted therapeutic management.
Live mucosal vaccination stimulates potent protection via varied CD4+ and CD8+ T cell subsets against wild-type Brucella melitensis 16M challenge
Authors: Goodwin Zakia I. , Yang Xinghong , Hoffman Carol , Pascual David W.
Published: October 2022

Re-emerging zoonotic pathogenBrucellaspp. continues to impact developing countries and persists in expanding populations of wildlife species in the US, constantly threatening infection of our domestic herds. The development of improved animal and human vaccines remains a priority. In this study, immunity to a novel live attenuatedB. melitensisstrain, termed znBM-mC, was characterized. An oral prime, intranasal (IN) boost strategy conferred exquisite protection against pulmonary challenge, with wild-type (wt)B. melitensisproviding nearly complete protection in the lungs and spleens from brucellae colonization. Vaccination with znBM-mC showed an IFN-γ+CD8+T-cell bias in the lungs as opposed to Rev 1-vaccinated mice showing IFN-γ+CD4+T-cell inclination. Lung CD4+and CD8+effector memory T cells (TEMs) increased over 200-fold; and lung CD4+and CD8+resident memory T cells (TRMs) increased more than 250- and 150-fold, respectively. These T cells served as the primary producers of IFN-γ in the lungs, which was essential for vaccine clearance and the predominant cytokine generated pre-and post-challenge with wtB. melitensis16M; znBM-mC growth could not be arrested in IFN-γ−/−mice. Increases in lung TNF-α and IL-17 were also induced, with IL-17 being mostly derived from CD4+T cells. Vaccination of CD4−/−, CD8−/−, and B6 mice with znBM-mC conferred full protection in the lungs and spleens post-pulmonary challenge with virulentB. melitensis;vaccination of IL-17−/−mice resulted in the protection of the lungs, but not the spleen. These data demonstrate the efficacy of mucosal vaccine administration for the generation of protective memory T cells against wtB. melitensis.
Tidal mitigation of offshore wind wake effects in coastal seas
Authors: Christiansen Nils , Daewel Ute , Schrum Corinna
Published: October 2022

With increasing offshore wind development, more and more marine environments are confronted with the effects of atmospheric wind farm wakes on hydrodynamic processes. Recent studies have highlighted the impact of the wind wakes on ocean circulation and stratification. In this context, however, previous studies indicated that wake effects appear to be attenuated in areas strongly determined by tidal energy. In this study, we therefore determine the role of tides in wake-induced hydrodynamic perturbations and assess the importance of the local hydrodynamic conditions on the magnitude of the emerging wake effects on hydrodynamics. By using an existing high-resolution model setup for the southern North Sea, we performed different scenario simulations to identify the tidal impact. The results show the impact of the alignment between wind and ocean currents in relation to the hydrodynamic changes that occur. In this regard, tidal currents can deflect emerging changes in horizontal surface currents and even mitigate the mean changes in horizontal flow due to periodic perturbations of wake signals. We identified that, particularly in shallower waters, tidal stirring influences how wind wake effects translate to changes in vertical transport and density stratification. In this context, tidal mixing fronts can serve as a natural indicator of the expected magnitude of stratification changes due to atmospheric wakes. Ultimately, tide-related hydrodynamic features, like periodic currents and mixing fronts, influence the development of wake effects in the coastal ocean. Our results provide important insights into the role of hydrodynamic conditions in the impact of atmospheric wake effects, which are essential for assessing the consequences of offshore wind farms in different marine environments.
Assessing the quantity and quality of marine protected areas in the Mariana Islands
Authors: Mana‘oakamai Johnson Steven , Villagomez Angelo O.
Published: October 2022

Marine protected areas (MPAs) are ubiquitous in global ocean conservation and play a pivotal role in achieving local, national, and regional area-based conservation targets. Often, such targets are merely met on “paper” and lack the political or managerial resources to produce positive conservation outcomes. Here, we apply the MPA Guide – a framework for assessing the quantity and quality of marine protected areas – to Guam and the Commonwealth of the Northern Mariana Islands (CNMI), two U.S. territories in the Western Pacific. We reviewed the enabling legislation and applicable management documents for all MPAs (n=18). We found that all but three (3) MPAs in the Mariana Islands are actively managed, and these areas are either fully or highly protected – the highest tier of the MPA Guide. Lightly protected areas are associated with high use/high-density tourism activities. Total area protected varies at the jurisdictional scale: 0.83% of Guam’s territorial waters (out to 12 nm) and 23.73% of EEZ under some spatial management; CNMI has 20.39% of territorial waters and 25.91% of EEZ in MPAs. These results emphasize the importance of quality, quantity, and scale when determining effective conservation, especially in overlapping and contested jurisdictional authority areas.
Acute hypoxia changes the gene expression profiles and alternative splicing landscape in gills of spotted sea bass (Lateolabrax maculatus)
Authors: Ren Yuhang , Tian Yuan , Mao Xuebin , Wen Haishen , Qi Xin , Li Jinku , Li Jifang , Li Yun
Published: October 2022

Hypoxia is one of the most important environmental stressors in aquatic ecosystems. To deal with the hypoxia environment, fishes exhibit a series of physiological and molecular responses to maintain homeostasis and organism functions. In the present study, hypoxia-induced changes in gene expression profiles and alternative splicing (AS) events in spotted sea bass (Lateolabrax maculatus), a promising marine-culture fish species in China, were thoroughly investigated by RNA-Seq analysis. A total of 1,242, 1,487 and 1,762 differentially expressed genes (DEGs) were identified at 3 h, 6 h and 12 h in gills after hypoxia stress. Functional enrichment analysis by KEGG and GSEA demonstrated that HIF signal network system was significantly activated and cell cycle process was remarkably suppressed in response to hypoxia. According to the temporal gene expression profiles, six clusters were generated and protein-protein interaction (PPI) networks were constructed for the two clusters that enriched with hypoxia-induced (cluster 2) or -suppressed genes (cluster 5), respectively. Results showed that HIF signaling related genes including vegfa, igf1, edn1, cox2b, cxcr4b, ctnnb1, and slc2a1a, were recognized as hubs in cluster 2, while mcm2, chek1, pole, mcm5, pola1, and rfc4, that tightly related to cell cycle, were down-regulated and considered as hubs in cluster 5. Furthermore, a total of 410 differential alternative splicing (DAS) genes were identified after hypoxia, which were closely associated with spliceosome. Of them, 63 DAS genes also showed differentially expressed levels after hypoxia, suggesting that their expression changes might be regulated by AS mechanism. This study revealed the key biological pathways and AS events affected by hypoxia, which would help us to better understand the molecular mechanisms of hypoxia response in spotted sea bass and other fish species.
No apparent cost of disease resistance on reproductive output in Acropora cervicornis genets used for active coral reef restoration in Florida
Authors: Koch Hanna R. , Azu Yuen , Bartels Erich , Muller Erinn M.
Published: October 2022

As assisted sexual reproduction interventions continue to become embedded within coral reef restoration initiatives, it is important to understand the potential for trade-offs between key traits like reproductive output and disease resistance. Oocyte size and fecundity, quantitative measures of reproductive output and important life-history traits, can be used as proxies for coral reproductive success and health. Sexual reproduction, particularly gametogenesis, is an energetically costly process and at the physiological level, trade-offs are caused by competitive allocation of limited resources to various functions. However, resource allocation trade-offs may also have a genetic basis, and thus, different genets may differ in these aspects. Therefore, the purpose of this study was to assess the reproductive output of A. cervicornis genets with known white-band disease resistance or susceptibility by quantifying the number and size of oocytes within colonies maintained within Mote Marine Laboratory’s offshore coral spawning nursery in the Lower Florida Keys, USA. We also quantified the number of eggs and sperm packaged within gamete bundles that were collected during the August 2020 spawning event. Consistent with previous studies, we found a positive correlation between colony size and fecundity. Interestingly though, we found no evidence for a trade-off between disease resistance and reproductive output and instead found a negative correlation between disease susceptibility and oocyte size. These data are relevant for population management interventions and for managing broodstock used for active restoration where a suite of corals with different genotypes and phenotypes are continuously propagated and outplanted. Having a more comprehensive understanding of the fitness differences among candidates can help guide such efforts and ensure that a diversity of fit genets is used for restoration, which should ultimately support greater adaptive potential and population resilience.
Case report: Diagnosis of hereditary hemorrhagic telangiectasia (Osler Weber Rendu Syndrome) in a 23-year-old male presented with anemia and thrombocytopenia and its response to bevacizumab
Authors: Yunus Hamza , Amin Said , Haq Furqan Ul , Ali Waqar , Hamid Tanveer , Ali Wajid , Ullah Basharat , Bai Payal
Published: October 2022

Osler Weber Rendu Syndrome (OWS) is characterized by the development of abnormally dilated blood vessels, which manifest as arteriovenous shunts (pulmonary, gastrointestinal, hepatic, and cerebral) and mucocutaneous telangiectasias (lips, tongue, and fingertips). It is an autosomal dominant disease with a defect in transforming growth factor beta superfamily genes. This defect results in increased angiogenesis and disruption of vessel wall integrity. The disease remains underreported, with occasional history of recurrent epistaxis, iron deficiency anemia, and gastrointestinal bleeding in moderate to severe cases. Diagnosis is based on clinical presentation and confirmed by genetic testing. Various local (nasal saline, air humidification, laser ablation, and electric cauterization for epistaxis and endoscopic Argon Plasma Coagulation-APC for active GI bleeding), surgical, and systemic (tranexamic acid and antiangiogenic agents like bevacizumab and thalidomide) treatment options are used depending upon disease severity. Here, we present a case with recurrent gastrointestinal bleeding refractory to endoscopic APC ablation and thalidomide and severe symptomatic anemia requiring multiple packed red cell transfusions. The patient was ultimately started on bevacizumab, to which he had a good response and has remained in remission for 8 months as of now. This case emphasizes the need to have a low threshold of suspicion to diagnose HHT and start targeted therapy like bevacizumab early on in moderate to severe cases of HHT rather than just relying on temporizing palliative measures like ablation, cauterization, and tranexamic acid.
First insights into the prokaryotic community structure of Lake Cote, Costa Rica: Influence on nutrient cycling
Authors: Brenes-Guillén Laura , Vidaurre-Barahona Daniela , Avilés-Vargas Lidia , Castro-Gutierrez Victor , Gómez-Ramírez Eddy , González-Sánchez Kaylen , Mora-López Marielos , Umaña-Villalobos Gerardo , Uribe-Lorío Lorena , Hassard Francis
Published: October 2022

Prokaryotic diversity in lakes has been studied for many years mainly focusing on community structure and how the bacterial assemblages are driven by physicochemical conditions such as temperature, oxygen, and nutrients. However, little is known about how the composition and function of the prokaryotic community changes upon lake stratification. To elucidate this, we studied Lake Cote in Costa Rica determining prokaryotic diversity and community structure in conjunction with physicochemistry along vertical gradients during stratification and mixing periods. Of the parameters measured, ammonium, oxygen, and temperature, in that order, were the main determinants driving the variability in the prokaryotic community structure of the lake. Distinct stratification of Lake Cote occurred (March 2018) and the community diversity was compared to a period of complete mixing (March 2019). The microbial community analysis indicated that stratification significantly altered the bacterial composition in the epi-meta- and hypolimnion. During stratification, the Deltaproteobacteria, Chloroflexi, Bacteroidetes, Nitrospirae, and Euryarchaeota were dominant in the hypolimnion yet largely absent in surface layers. Among these taxa, strict or facultative anaerobic bacteria were likely contributing to the lake nitrogen biogeochemical cycling, consistent with measurements of inorganic nitrogen measurements and microbial functional abundance predictions. In general, during both sampling events, a higher abundance of Alphaproteobacteria, Betaproteobacteria, Actinobacteria, and Cyanobacteria was found in the oxygenated layers. Lake Cote had a unique bacterial diversity, with 80% of Amplicon Sequence Variant (ASV) recovered similar to unclassified/uncultured strains and exhibits archetypal shallow lake physicochemical but not microbial fluctuations worthy of further investigation. This study provides an example of lake hydrodynamics impacts to microbial community and their function in Central American lakes with implications for other shallow, upland, and oligotrophic lake systems.
Antimicrobial resistance of microorganisms present in periodontal diseases: A systematic review and meta-analysis
Authors: Abe Flávia Casale , Kodaira Katia , Motta Cristiane de Cássia Bergamaschi , Barberato-Filho Silvio , Silva Marcus Tolentino , Guimarães Caio Chaves , Martins Carolina Castro , Lopes Luciane Cruz
Published: October 2022

Objective

The aim of this study was to estimate the antimicrobial resistance in microorganisms present in periodontal diseases.

Methods

A systematic review was conducted according to the PRISMA statement. The MEDLINE (PubMed/Ovid), EMBASE, BVS, CINAHL, and Web of Science databases were searched from January 2011 to December 2021 for observational studies which evaluated the antimicrobial resistance in periodontal diseases in permanent dentition. Studies that allowed the antimicrobial consumption until the time of sample collection, studies that used laboratory acquired strains, studies that only characterized the microbial strain present, assessment of cellular morphological changes, sequencing system validation, and time series were excluded. Six reviewers, working in pairs and independently, selected titles, abstracts, and full texts extracting data from all studies that met the eligibility criteria: characteristics of patients, diagnosis of infection, microbial species assessed, antimicrobial assessed, identification of resistance genes, and virulence factors. “The Joanna Briggs Institute” critical appraisal for case series was adapted to assess the risk of bias in the included studies.

Results

Twenty-four studies (N = 2.039 patients) were included. Prevotella and Porphyromonas species were the most cited microorganisms in the included studies, and the virulence factors were related to Staphylococcus aureus. The antimicrobial reported with the highest frequency of resistance in the included studies was ampicillin (39.5%) and ciprofloxacin showed the lowest frequency of resistance (3.4%). The most cited genes were related to macrolides. The quality of the included studies was considered critically low.

Conclusion

No evidence was found regarding the profile of antimicrobial resistance in periodontal diseases, requiring further research that should focus on regional population studies to address this issue in the era of increasing antimicrobial resistance.

Clinical relevance

The knowledge about the present microorganism in periodontal diseases and their respective antimicrobial resistance profiles should guide dentists in prescribing complementary therapy for these infections.

Systematic review registration

[http://dx.doi.org/10.1097/MD.0000000000013158], identifier [CRD42018077810].
Characterization and antagonistic potentials of selected rhizosphere Trichoderma species against some Fusarium species
Authors: Olowe Olumayowa Mary , Nicola Lidia , Asemoloye Micheal Dare , Akanmu Akinlolu Olalekan , Sobowale Ayodele Adegboyega , Babalola Olubukola Oluranti
Published: October 2022

Trichoderma fungi have been proved as efficient bioagents with great antifungal properties while many species in the plant’s rhizospheres have been characterized as plant growth-promoting agents. However, many rhizosphere Trichoderma are yet to be fully explored for plant disease management. In this study, Trichoderma species were isolated from the rhizosphere of maize, banana, and cassava, and their biocontrol potentials were screened against some Fusarium species from oak leaves (F2B and F3) and laboratory cultures (Fus 296 and Fus 294). The isolated rhizosphere Trichoderma were identified as Trichoderma virens 1 (TCIV), T. virens 2 (TCVII), T. virens 3 (TMSI), T. hazianum strain 1 (TCVI), T. harzianum strain 2 (TCVIII), T. erinaceum (TMZI), and T. koningiopsis (TMZII). The dual culture experiment recorded the highest percentage inhibition in TMZII against OakF2B (31.17%), TCVIII against Fus 294 (45.18%), TMZI against Fus 296 (47.37%), while TCIV was most effective against Oak F3 (44.15%). Among the Trichoderma culture filtrates evaluated, TCIV showed the highest percentage inhibition against Oak F3 (52.39%), Oak F2B (48.54%), Fus 294 (46.65%), and Fus 296 (44.48%). All the Trichoderma isolates demonstrated expressed varying levels of antagonism against the Fusarium pathogens in vitro.
Microglial repopulation alleviates age-related decline of stable wakefulness in mice
Authors: Liu Hanxiao , Badawy Mohamed , Sun Shaoqin , Cruz George , Ge Shaoyu , Xiong Qiaojie
Published: October 2022

Changes in wake/sleep architecture have been observed in both aged human and animal models, presumably due to various functional decay throughout the aging body particularly in the brain. Microglia have emerged as a modulator for wake/sleep architecture in the adult brain, and displayed distinct morphology and activity in the aging brain. However, the link between microglia and age-related wake/sleep changes remains elusive. In this study, we systematically examined the brain vigilance and microglia morphology in aging mice (3, 6, 12, and 18 months old), and determined how microglia affect the aging-related wake/sleep alterations in mice. We found that from young adult to aged mice there was a clear decline in stable wakefulness at nighttime, and a decrease of microglial processes length in various brain regions involved in wake/sleep regulation. The decreased stable wakefulness can be restored following the time course of microglia depletion and repopulation in the adult brain. Microglia repopulation in the aging brain restored age-related decline in stable wakefulness. Taken together, our findings suggest a link between aged microglia and deteriorated stable wakefulness in aged brains.
Therapeutic hypothermia for stroke: Unique challenges at the bedside
Authors: You Je Sung , Kim Jong Youl , Yenari Midori A.
Published: October 2022

Therapeutic hypothermia has shown promise as a means to improving neurological outcomes at several neurological conditions. At the clinical level, it has been shown to improve outcomes in comatose survivors of cardiac arrest and in neonatal hypoxic ischemic encephalopathy, but has yet to be convincingly demonstrated in stroke. While numerous preclinical studies have shown benefit in stroke models, translating this to the clinical level has proven challenging. Major obstacles include cooling patients with typical stroke who are awake and breathing spontaneously but often have significant comorbidities. Solutions around these problems include selective brain cooling and cooling to lesser depths or avoiding hyperthermia. This review will cover the mechanisms of protection by therapeutic hypothermia, as well as recent progress made in selective brain cooling and the neuroprotective effects of only slightly lowering brain temperature. Therapeutic hypothermia for stroke has been shown to be feasible, but has yet to be definitively proven effective. There is clearly much work to be undertaken in this area.
White matter alterations in drug-naïve children with Tourette syndrome and obsessive-compulsive disorder
Authors: Bharti Komal , Conte Giulia , Tommasin Silvia , Giannì Costanza , Suppa Antonio , Mirabella Giovanni , Cardona Francesco , Pantano Patrizia
Published: October 2022

Tourette syndrome (TS) and early-onset obsessive-compulsive disorder (OCD) are frequently associated and conceptualized as distinct phenotypes of a common disease spectrum. However, the nature of their relationship is still largely unknown on a pathophysiological level. In this study, early structural white matter (WM) changes investigated through diffusion tensor imaging (DTI) were compared across four groups of drug-naïve children: TS-pure (n = 16), TS+OCD (n = 14), OCD (n = 10), and 11 age-matched controls. We analyzed five WM tracts of interest, i.e., cortico-spinal tract (CST), anterior thalamic radiations (ATR), inferior longitudinal fasciculus (ILF), corpus callosum (CC), and cingulum and evaluated correlations of DTI changes to symptom severity. Compared to controls, TS-pure and TS+OCD showed a comparable pattern of increased fractional anisotropy (FA) in CST, ATR, ILF and CC, with FA changes displaying negative correlation to tic severity. Conversely, in OCD, FA decreased in all WM tracts (except for the cingulum) compared to controls and negatively correlated to symptoms. We demonstrate different early WM microstructural alterations in children with TS-pure/TS+OCD as opposed to OCD. Our findings support the conceptualization of TS+OCD as a subtype of TS while suggesting that OCD is characterized by independent pathophysiological mechanisms affecting WM development.
Efficacy of auricular plaster therapy for sleep disorders in preschool children with autism spectrum disorders: Study protocol for a randomized controlled trial
Authors: Duan Duoxi , He Lin , Chen Hong , Lei Ying , Wu Wei , Li Tao
Published: October 2022

Background

Children with autism spectrum disorders (ASDs) suffer from sleep disorders to a considerable degree; however, there is no safe and effective treatment available in clinical practice. The objective of the trial is to assess the clinical effectiveness of auricular plaster therapy (APT) in treating sleep disorders in children with ASD.

Method

This is a single-center, patient-assessor blind, randomized controlled trial. A total of 44 preschool children with sleep disorders with ASD will be included in this study. Eligible participants will be randomly assigned to either the auricular plaster group or the sham auricular plaster group in a 1:1 ratio. Participants in the different groups will receive APT or sham APT, respectively, for a total of 30 sessions over 30 days. The primary outcome includes the Children's Sleep Habits Questionnaire (CSHQ), while secondary outcomes include the Autism Behavior Checklist (ABC) and polysomnography (PSG) for total sleep time, sleep latency, awakening duration, and sleep structures. The CSHQ and ABC will be assessed at baseline, 10, 20, 30, 60, 90, and 120 days after randomization, whereas PSG will be assessed at baseline and 30 days after randomization. The follow-up period will be scheduled to be 60, 90, and 120 days after randomization.

Discussion

The results of this study may provide evidence of the efficacy of APT, as well as offer new alternatives for the treatment of sleep disorders in children with ASD.

Trial registration

CHiCTR.org.cn (ChiCTR2100048257). Registered on July 5, 2021.