bluuebunny/crossref_metadata_2025 huggingface dataset:
Urban and Rural Disparities in a WeChat-Based Smoking Cessation Intervention among Chinese Smokers
Authors: Luo Ting , Li Mirandy , Williams Donna , Fritz Jackson , Phillippi Stephen , Yu Qingzhao , Kantrow Stephen , Chen Liwei , Chen Yongchun , Beiter Kaylin , Tseng Tung-Sung
Published: June 2021

Introduction: Tobacco use, which is directly responsible for 10% of total deaths per year globally, remains consistently high, with approximately 20% of the population reporting regular consumption globally. Moreover, health disparities regarding tobacco consumption and smoking cessation are growing between rural and urban populations worldwide. Social media interventions for tobacco cessation may effectively reach both groups. The objective of this study was to evaluate the efficacy of a WeChat-delivered smoking cessation intervention among rural and urban Chinese smokers, and to assess moderating variables that may contribute to differential intervention efficacy. Methods: WeChat was used to recruit smokers into this intervention study between 1 July and 5 August 2019. Participants were randomized to one of three intervention schedules: participants in the Standard Group and the Enhanced Group received 20 smoking-related messages over 2 weeks, whereas participants in the Enhanced Group received an extra 6 oral health-related messages for one week. Participants in the control group received 20 smoking-related messages after the post-intervention assessment. Participants completed questionnaires at baseline and at 4 weeks follow-up. Our primary outcome was smoking cessation stage of change and secondary outcome was 24-h point prevalence abstinence (PPA). Urban and rural areas were based on self-reported living areas. Chi-squared test, Fisher’s exact test, ANOVA test, linear regression, and logistic regression were used for analysis. Results: Overall, 403 participants completed the intervention (233 rural, 107 suburban, 63 urban). Compared to urban participants, rural participants were more likely to have progressed to a later stage of change (β = 0.40, 95% CI: 0.13, 0.67) and to report higher 24-h PPA rates at follow-up (aOR = 3.23, 95% CI: 1.36, 7.68). After stratification by living area, the intervention effects in stage of change and 24-h PPA rate at follow-up were only found in the urban subgroup. Discussion: Smokers who lived in rural areas reported better smoking cessation outcomes compared with urban smokers; however, the efficacy of a WeChat-based smoking cessation intervention was only found for participants living in an urban area. WeChat based smoking cessation interventions should be used to promote smoking cessation in urban, suburban, and rural areas.
Midwives’ Experiences with and Perspectives on Online (Nutritional) Counselling and mHealth Applications for Pregnant Women; an Explorative Qualitative Study
Authors: Wit Renate F. , Lucassen Desiree A. , Beulen Yvette H. , Faessen Janine P. M. , Bos-de Vos Marina , van Dongen Johanna M. , Feskens Edith J. M. , Wagemakers Annemarie , Brouwer-Brolsma Elske M.
Published: June 2021

Prenatal nutrition is a key predictor of early-life development. However, despite mass campaigns to stimulate healthy nutrition during pregnancy, the diet of Dutch pregnant women is often suboptimal. Innovative technologies offer an opportunity to develop tailored tools, which resulted in the release of various apps on healthy nutrition during pregnancy. As midwives act as primary contact for Dutch pregnant women, the goal was to explore the experiences and perspectives of midwives on (1) nutritional counselling during pregnancy, and (2) nutritional mHealth apps to support midwifery care. Analyses of eleven in-depth interviews indicated that nutritional counselling involved the referral to websites, a brochure, and an app developed by the Dutch Nutrition Centre. Midwives were aware of the existence of other nutritional mHealth apps but felt uncertain about their trustworthiness. Nevertheless, midwives were open towards the implementation of new tools providing that these are trustworthy, accessible, user-friendly, personalised, scientifically sound, and contain easy-digestible information. Midwives stressed the need for guidelines for professionals on the implementation of new tools. Involving midwives early-on in the development of future nutritional mHealth apps may facilitate better alignment with the needs and preferences of end-users and professionals, and thus increase the likelihood of successful implementation in midwifery practice.
Positive Mental Health of Finnish People Living Alone: The Role of Circumstantial Factors and Leisure-Time Activities
Authors: Pasanen Tytti P. , Tamminen Nina , Martelin Tuija , Solin Pia
Published: June 2021

Living alone has become more common across Europe. Past research has consistently identified living alone as a risk factor for poor mental health while evidence on the positive dimension(s) of mental health has been scarce. Positive mental health has been associated with rather stable circumstantial factors, such as socio-economic characteristics and social relationships, and day-to-day activities in the form of leisure participation, in general populations. In this study, our objective was to assess these relationships among people living alone. We specified a structural equation model in a random sample of Finnish people living alone (n = 884), with the circumstantial factors as (exogenous) explanatory variables, participation in various leisure activities as mediators, and positive mental health as the outcome. In the model, more frequent engagement in several leisure-time activities, including being in contact with family/friends and physical activity in nature, were positively associated with positive mental health. The circumstantial factors that most strongly explained both leisure participation and positive mental health were the number of friends, being in a relationship, and having no limiting illnesses. In conclusion, among Finnish people living alone, social and functional factors appear to be more strongly associated with leisure participation and positive mental health than socio-economic factors.
The Impact Assessment of CuO Nanoparticles on the Composition and Ultrastructure of Triticum aestivum L.
Authors: Lung Ildiko , Opriş Ocsana , Soran Maria-Loredana , Culicov Otilia , Ciorîță Alexandra , Stegarescu Adina , Zinicovscaia Inga , Yushin Nikita , Vergel Konstantin , Kacso Irina , Borodi Gheorghe , Pârvu Marcel
Published: June 2021

In the present study, the effects of copper oxide nanoparticles (CuO NPs) on bioactive compounds, the ultrastructural modifications which can occur, and elemental content of wheat were investigated. Changes in the wheat plants grown in presence or absence of CuO NPs were estimated. The application of CuO NPs decreased the amounts of chlorophylls and carotenoids and increased the amounts of polyphenols and antioxidant capacity. Ultrastructural analysis showed that the plants treated with CuO NPs were negatively affected. Soil amending completely inhibited the accumulation of seventeen elements, while K, Br, Al, and Zn were accumulated and Cl, Na, Ba, and Sr content decreased in wheat samples, regardless of the type of NPs applied. The application of chemically obtained NPs induced the most significant changes, completely blocking the assimilation of Fe, Mo, As, Sb, and Sm, and favoring much higher accumulation of Br than biogenic NPs. The decrease in chlorophylls and carotenoids is correlated with increase in antioxidant capacity, and occurs with increase of Mo, Al, Mg, K, Zn, and Ca content. The behavior of total polyphenols is correlated with Br content, and antagonist to Al behavior. From the point of view of bioactive compounds, the most affected plants were those that grew in the presence of CuO-NP-cel, while from the point of view of elementary analysis, the most affected plants were those grown in the presence of CuO-NP. By corroborating the obtained results, it was found that the CuO NPs have a negative effect on wheat plants.
The MATES Case Management Model: Presenting Problems and Referral Pathways for a Novel Peer-Led Approach to Addressing Suicide in the Construction Industry
Authors: Doran Christopher M. , Wittenhagen Lisa , Heffernan Edward , Meurk Carla
Published: June 2021

MATES in Construction (MATES) is a multimodal, peer-led, workplace suicide prevention and early intervention program developed to reduce the risk of suicide among construction industry workers through active facilitation of appropriate support. The MATES case management model provides an example of a nonclinical service for meeting the needs of individuals in the construction industry who, while at elevated risk of mental health problems and suicidality, are traditionally less likely to seek help. The aim of this research was to conduct an evaluation of the MATES case management database to quantify service demand, and to examine the demographic, occupational profile, presenting issues, referral pathways, and perceived benefit of case management among individuals who used this service. The research reports on routinely collected data from the Queensland MATES case management database, which contains records on 3759 individuals collected over the period 2010–2018, and findings from a small and opportunistic exit survey undertaken with 14 clients in 2019. Overall, findings suggest that the demand for case management through MATES has increased significantly and that clients felt that their needs and concerns were appropriately addressed. The most common presenting issues were relationship, work, and family problems, suicide, and mental health concerns. Findings confirm that causes of distress extend beyond the realm of mental disorder and span a range of psychosocial issues. Significantly, it offers an approach that may divert individuals in crisis away from presenting to over-run emergency departments, and towards services that are more equipped to meet their individual needs.
Development and Psychometric Assessment of the Problematic QQ Use Scale among Adolescents
Authors: Liu Jintao , Ahmed Md Zahir , Ahmed Oli , Griffiths Mark D. , Chen Lili
Published: June 2021

The QQ social media platform is very popular among Chinese adolescents. As with other social media platforms (e.g., Facebook, Instagram, YouTube, etc.), there have been increasing reports that the use of QQ can be potentially problematic to a minority of users. However, unlike these other social media platforms, there is currently no scale to assess the risk of problematic QQ use. The present study developed the Problematic QQ Use Scale (PQQUS) among Chinese adolescents based on six core criteria of behavioral addiction (salience, tolerance, mood modification, loss of control, withdrawal, and conflict) that have been used in the development of other social media addiction scales. The scale was administered to a sample of 1008 Chinese school children to assess its psychometric properties, utilizing both classical test theory and item response theory. The analysis demonstrated that the PQQUS had good item discrimination indices relating to both CTT and IRT. The CFA results and Loevinger’s H-coefficient suggested the PQQUS had a unidimensional factor structure. The PQQUS had good internal reliability, good composite reliability, and good concurrent validity (based on correlations with measures of anxiety, depression, self-esteem, and life satisfaction). The invariance testing between boys and girls suggested this scale is a valid assessment tool for both groups. Overall, the PQQUS is a psychometrically robust tool for assessing problematic QQ use and will have a key role in further research on problematic QQ use among Chinese adolescents.
Trade-in Strategy and Competition between Two Independent Remanufacturers
Authors: Feng Zhangwei , Luo Na , Liu Yanping
Published: June 2021

Trade-in strategy is a common mode of promotion for firms taking part in the circular economy because it encourages consumers to buy remanufactured products, via a “trade-old-for-remanufactured” framework. This paper studies trade-in strategy by developing game models for a closed-loop supply chain with one manufacturer and two independent remanufacturers. The former is responsible for producing and selling new products and the latter two compete with each other for the collection of used products and the sales of remanufactured products. Unlike the extant literature, this paper focuses on the competition between two independent remanufacturers (IRs) and studies on how holder segment (whether or not to own used products) and competition affects the trade-in strategy. It finds that the proportion of holders on the remanufactured product prices of the IR1 and IR2 have a negative correlation. Conversely, the impact of the proportion of holders on the IR1’s and IR2’s demands (and on their profits) is the opposite. The trade-in strategy generates more benefits for the IR1 only when the proportion of holders is sufficiently high. In addition, when consumers experience a greater difference in durability between remanufactured products and original new products, trade-in strategy is more welcomed by consumers, which in turn, generates more benefits for the IR1.
The Knowledge, Practice and Attitudes of Nurses Regarding Physical Restraint: Survey Results from Psychiatric Inpatient Settings
Authors: Lee Tsz-Kai , Välimäki Maritta , Lantta Tella
Published: June 2021

There is a considerable amount of literature describing how nurses’ knowledge contributes to their attitudes and practices related to patient physical restraint. However, whether or not there have been any improvements in nurses’ knowledge levels, attitudes or practices regarding physical restraint during the past few years is unknown. A survey was conducted on nurses (n = 133) in one psychiatric hospital in Hong Kong (n = 98, response rate = 74%). The data were analyzed using independent t-tests, ANOVA, a Mann–Whitney U test, a Kruskal–Wallis test and Spearman’s rho. In general, nurses had good restraint-related knowledge with satisfactory attitudes and practices, although their knowledge levels, attitudes, and practices regarding restraint varied. Having a higher age, seniority, and education level contributed to a higher restraint-related knowledge level. Male nurses demonstrated more desirable practices (i.e., care of restrained patients), while nurses with a higher education level were more likely to avoid restraint. Nurses’ restraint-related knowledge positively correlated with restraint practices. Although nurses’ knowledge levels, attitudes, and practices regarding restraint were found to be satisfactory, more training efforts should focus on young nurses working in psychiatric settings with less work experience and lower education levels. As some nurses seem to favor the use of restraint with limited reflection, more studies are needed to verify nurses’ emotions and how their emotions influence the use of restrictive practices.
The Impact of the Lockdown Caused by the COVID-19 Pandemic on the Fine Particulate Matter (PM2.5) Air Pollution: The Greek Paradigm
Authors: Kotsiou Ourania S. , Saharidis Georgios K. D. , Kalantzis Georgios , Fradelos Evangelos C. , Gourgoulianis Konstantinos I.
Published: June 2021

Introduction: Responding to the coronavirus pandemic, Greece implemented the largest quarantine in its history. No data exist regarding its impact on PM2.5 pollution. We aimed to assess PM2.5 levels before, during, and after lockdown (7 March 2020–16 May 2020) in Volos, one of Greece’s most polluted industrialized cities, and compare PM2.5 levels with those obtained during the same period last year. Meteorological conditions were examined as confounders. Methods: The study period was discriminated into three phases (pre-lockdown: 7 March–9 March, lockdown: 10 March–4 May, and post-lockdown period: 5 May–16 May). A wireless sensors network was used to collect PM2.5, temperature, relative humidity, rainfall, and wind speed data every 2 s. Results: The lockdown resulted in a significant drop of PM2.5 by 37.4% in 2020, compared to 2019 levels. The mean daily concentrations of PM2.5 exceeded the WHO’s guideline value for 24-h mean levels of PM2.5 35% of the study period. During the strictest lockdown (23 March to 4 May), the mean daily PM2.5 levels exceeded the standard 41% of the time. The transition from the pre-lockdown period into lockdown or post-lockdown periods was associated with lower PM2.5 concentrations. Conclusions: A reduction in the mean daily PM2.5 concentration was found compared to 2019. Lockdown was not enough to avoid severe exceedances of air pollution in Volos.
Machine Learning for Analyzing Non-Countermeasure Factors Affecting Early Spread of COVID-19
Authors: Janko Vito , Slapničar Gašper , Dovgan Erik , Reščič Nina , Kolenik Tine , Gjoreski Martin , Smerkol Maj , Gams Matjaž , Luštrek Mitja
Published: June 2021

The COVID-19 pandemic affected the whole world, but not all countries were impacted equally. This opens the question of what factors can explain the initial faster spread in some countries compared to others. Many such factors are overshadowed by the effect of the countermeasures, so we studied the early phases of the infection when countermeasures had not yet taken place. We collected the most diverse dataset of potentially relevant factors and infection metrics to date for this task. Using it, we show the importance of different factors and factor categories as determined by both statistical methods and machine learning (ML) feature selection (FS) approaches. Factors related to culture (e.g., individualism, openness), development, and travel proved the most important. A more thorough factor analysis was then made using a novel rule discovery algorithm. We also show how interconnected these factors are and caution against relying on ML analysis in isolation. Importantly, we explore potential pitfalls found in the methodology of similar work and demonstrate their impact on COVID-19 data analysis. Our best models using the decision tree classifier can predict the infection class with roughly 80% accuracy.
Modelling the Relationship between the Nature of Work Factors and Driving Performance Mediating by Role of Fatigue
Authors: Al-Mekhlafi Al-Baraa Abdulrahman , Isha Ahmad Shahrul Nizam , Chileshe Nicholas , Abdulrab Mohammed , Saeed Anwar Ameen Hezam , Kineber Ahmed Farouk
Published: June 2021

Driving fatigue is a serious issue for the transportation sector, decreasing the driver’s performance and increasing accident risk. This study aims to investigate how fatigue mediates the relationship between the nature of work factors and driving performance. The approach included a review of the previous studies to select the dimensional items for the data collection instrument. A pilot test to identify potential modification to the questionnaire was conducted, then structural equation modelling (SEM) was performed on a stratified sample of 307 drivers, to test the suggested hypotheses. Based on the results, five hypotheses have indirect relationships, four of which have a significant effect. Besides, the results show that driving fatigue partially mediates the relationship between the work schedule and driving performance and fully mediates in the relationship between work activities and driving performance. The nature of work and human factors is the most common reason related to road accidents. Therefore, the emphasis on driving performance and fatigue factors would thereby lead to preventing fatal crashes and life loss.
Mixed POT-BM Approach for Modeling Unhealthy Air Pollution Events
Authors: Masseran Nurulkamal , Safari Muhammad Aslam Mohd
Published: June 2021

This article proposes a novel data selection technique called the mixed peak-over-threshold–block-maxima (POT-BM) approach for modeling unhealthy air pollution events. The POT technique is employed to obtain a group of blocks containing data points satisfying extreme-event criteria that are greater than a particular threshold u. The selected groups are defined as POT blocks. In parallel with that, a declustering technique is used to overcome the problem of dependency behaviors that occurs among adjacent POT blocks. Finally, the BM concept is integrated to determine the maximum data points for each POT block. Results show that the extreme data points determined by the mixed POT-BM approach satisfy the independent properties of extreme events, with satisfactory fitted model precision results. Overall, this study concludes that the mixed POT-BM approach provides a balanced tradeoff between bias and variance in the statistical modeling of extreme-value events. A case study was conducted by modeling an extreme event based on unhealthy air pollution events with a threshold u > 100 in Klang, Malaysia.
Correction: Ruiz et al. Social Environmental Antecedents of Athletes’ Emotions. Int. J. Environ. Res. Public Health 2021, 18, 4997
Authors: Ruiz Montse C. , Appleton Paul R. , Duda Joan L. , Bortoli Laura , Robazza Claudio
Published: June 2021

In the original article [...]
Family Support and Sociocultural Factors on Depression among Black and Latinx Sexual Minority Men
Authors: Boyd Donte T. , Ramos S. Raquel , Quinn Camille R. , Jones Kristian V. , Wilton Leo , Nelson LaRon E.
Published: June 2021

Family-based approaches are critical for improving health outcomes in sexual minority men (SMM) of color. Yet, it is unclear how family context, internalized homophobia, and stress influence mental health outcomes among sexual minority men of color. From a cross-sectional sample of 448 participants, aged 16–24 years, survey data were analyzed to examine rates of family social support, the perception of sexuality by family, the stressfulness of life events, internalized homophobia, and other contextual variables on depression using linear regression. Our results indicated that an 86% increase in family social support was related to a −0.14 decrease in depression (ß = −0.14, p = 0.004). In addition, SMM who were separated by family and friends because of their sexuality were statistically significant and positively associated with depression (ß = 0.09, p < 0.001). Findings from our study suggest that the influence from the microsystem is salient in modifying mental health outcomes for SMM of color.
The Effects of Interval Training and Continuous Training on Cardiopulmonary Fitness and Exercise Tolerance of Patients with Heart Failure—A Systematic Review and Meta-Analysis
Authors: Li Daxin , Chen Ping , Zhu Junying
Published: June 2021

Purpose: To investigate the effects of interval training (IT) as compared with continuous training (CT) on cardiorespiratory fitness and exercise tolerance of patients with heart failure (HF), with the aim to provide reasonable exercise prescriptions for patients with HF. Methods: Through searching electronic databases, randomized controlled studies were collected. The included studies were evaluated for methodological quality using the Cochrane risk of bias assessment tool, and statistical analyses were carried out using Review Manager 5.3 and Stata MP 15.1 software. Results: A total of seventeen randomized controlled trials (i.e., studies) with 617 patients were included. The meta-analysis showed that IT can improve a patient’s peak oxygen uptake (VO2peak) (MD = 2.08, 95% CI 1.16 to 2.99, p < 0.00001), left ventricular ejection fraction (LVEF) (MD =1.32, 95% CI 0.60 to 2.03, p = 0.0003), and 6-minute walk distance (6MWD) (MD = 25.67, 95% CI 12.87 to 38.47, p < 0.0001) as compared with CT. However, for respiratory exchange ratio (RER) (MD = 0.00, 95% CI −0.02 to 0.03, p = 0.81), CO2 ventilation equivalent slope (VE/VCO2 slope) (SMD = 0.04, 95% CI −0.23 to 0.31, p = 0.75), and resting heart rate (HRrest) (MD = 0.15, 95% CI −3.00 to 3.29, p = 0.93) there were no statistical significance. Conclusions: The evidence shows that IT is better than CT for improving the cardiorespiratory fitness and exercise tolerance of patients with HF. Moreover, an intensity of 60–80% peak heart rate of IT is the optimal choice for patients. It is hoped that, in the future, more well-designed studies would further expand the meta-analysis results.
Joint Optimization on Trajectory, Cache Placement, and Transmission Power for Minimum Mission Time in UAV-Aided Wireless Networks
Authors: Lan Tingting , Qin Danyang , Sun Guanyu
Published: June 2021

In recent years, due to the strong mobility, easy deployment, and low cost of unmanned aerial vehicles (UAV), great interest has arisen in utilizing UAVs to assist in wireless communication, especially for on-demand deployment in emergency situations and temporary events. However, UAVs can only provide users with data transmission services through wireless backhaul links established with a ground base station, and the limited capacity of the wireless backhaul link would limit the transmission speed of UAVs. Therefore, this paper designed a UAV-assisted wireless communication system that used cache technology and realized the transmission of multi-user data by using the mobility of UAVs and wireless cache technology. Considering the limited storage space and energy of UAVs, the joint optimization problem of the UAV’s trajectory, cache placement, and transmission power was established to minimize the mission time of the UAV. Since this problem was a non-convex problem, it was decomposed into three sub-problems: trajectory optimization, cache placement optimization, and power allocation optimization. An iterative algorithm based on the successive convex approximation and alternate optimization techniques was proposed to solve these three optimization problems. Finally, in the power allocation optimization, the proposed algorithm was improved by changing the optimization objective function. Numerical results showed that the algorithm had good performance and could effectively reduce the task completion time of the UAV.
Using Standardized Time Series Land Cover Maps to Monitor the SDG Indicator “Mountain Green Cover Index” and Assess Its Sensitivity to Vegetation Dynamics
Authors: De Simone Lorenzo , Navarro Dorian , Gennari Pietro , Pekkarinen Anssi , de Lamo Javier
Published: June 2021

SDG indicators are instrumental for the monitoring of countries’ progress towards sustainability goals as set out by the UN Agenda 2030. Earth observation data can facilitate such monitoring and reporting processes, thanks to their intrinsic characteristics of spatial extensive coverage, high spatial, spectral, and temporal resolution, and low costs. EO data can hence be used to regularly assess specific SDG indicators over very large areas, and to extract statistics at any given subnational level. The Food and Agriculture Organization of the United Nations (FAO) is the custodian agency for 21 out of the 231 SDG indicators. To fulfill this responsibility, it has invested in EO data from the outset, among others, by developing a new SDG indicator directly monitored with EO data: SDG indicator 15.4.2, the Mountain Green Cover Index (MGCI), for which the FAO produced initial baseline estimates in 2017. The MGCI is a very important indicator, allowing the monitoring of the health of mountain ecosystems. The initial FAO methodology involved visual interpretation of land cover types at sample locations defined by a global regular grid that was superimposed on satellite images. While this solution allowed the FAO to establish a first global MGCI baseline and produce MGCI estimates for the large majority of countries, several reporting countries raised concerns regarding: (i) the objectivity of the method; (ii) the difficulty in validating FAO estimates; (iii) the limited involvement of countries in estimating the MGCI; and (iv) the indicator’s limited capacity to account for forest encroachment due to agricultural expansion as well as the undesired expansion of green vegetation in mountain areas, resulting from the effect of global warming. To address such concerns, in 2020, the FAO introduced a new data collection approach that directly measures the indicator through a quantitative analysis of standardized land cover maps (European Space Agency Climate Change Initiative Land Cover maps—ESA CCI-LC). In so doing, this new approach addresses the first three of the four issues, while it also provides stronger grounds to develop a solution for the fourth issue—a solution that the FAO plans to present to the Interagency and Expert Group on SDG Indicators (IAEG-SDG) at its autumn 2021 session. This study (i) describes the new approach to estimate the MGCI indicator using ESA’s CCI-LC and products, (ii) assesses the accuracy of the new approach; (iii) reviews the limitations of the current SDG indicator definition to monitor progress towards SDG 15.4; and (iv) reflects on possible further adjustments of the indicator methodology in order to address them.
Sub-Chronic Effects of Slight PAH- and PCB-Contaminated Mesocosms in Paracentrotus lividus Lmk: A Multi-Endpoint Approach and De Novo Transcriptomic
Authors: Albarano Luisa , Zupo Valerio , Caramiello Davide , Toscanesi Maria , Trifuoggi Marco , Guida Marco , Libralato Giovanni , Costantini Maria
Published: June 2021

Sediment pollution is a major issue in coastal areas, potentially endangering human health and the marine environments. We investigated the short-term sublethal effects of sediments contaminated with polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) on the sea urchin Paracentrotus lividus for two months. Spiking occurred at concentrations below threshold limit values permitted by the law (TLVPAHs = 900 µg/L, TLVPCBs = 8 µg/L, Legislative Italian Decree 173/2016). A multi-endpoint approach was adopted, considering both adults (mortality, bioaccumulation and gonadal index) and embryos (embryotoxicity, genotoxicity and de novo transcriptome assembly). The slight concentrations of PAHs and PCBs added to the mesocosms were observed to readily compartmentalize in adults, resulting below the detection limits just one week after their addition. Reconstructed sediment and seawater, as negative controls, did not affect sea urchins. PAH- and PCB-spiked mesocosms were observed to impair P. lividus at various endpoints, including bioaccumulation and embryo development (mainly PAHs) and genotoxicity (PAHs and PCBs). In particular, genotoxicity tests revealed that PAHs and PCBs affected the development of P. lividus embryos deriving from exposed adults. Negative effects were also detected by generating a de novo transcriptome assembly and its annotation, as well as by real-time qPCR performed to identify genes differentially expressed in adults exposed to the two contaminants. The effects on sea urchins (both adults and embryos) at background concentrations of PAHs and PCBs below TLV suggest a need for further investigations on the impact of slight concentrations of such contaminants on marine biota.
Changes in Ethylene, ABA and Sugars Regulate Freezing Tolerance under Low-Temperature Waterlogging in Lolium perenne
Authors: Jurczyk Barbara , Pociecha Ewa , Janowiak Franciszek , Dziurka Michał , Kościk Izabela , Rapacz Marcin
Published: June 2021

Plant overwintering may be affected in the future by climate change. Low-temperature waterlogging, associated with a predicted increase in rainfall during autumn and winter, can affect freezing tolerance, which is the main component of winter hardiness. The aim of this study was to elucidate the mechanism of change in freezing tolerance caused by low-temperature waterlogging in Lolium perenne, a cool-season grass that is well adapted to a cold climate. The work included: (i) a freezing tolerance test (plant regrowth after freezing); (ii) analysis of plant phytohormones production (abscisic acid [ABA] content and ethylene emission); (iii) measurement of leaf water content and stomatal conductance; (iv) carbohydrate analysis; and (v) analysis of Aco1, ABF2, and FT1 transcript accumulation. Freezing tolerance may be improved as a result of cold waterlogging. The mechanism of this change is reliant on multifaceted actions of phytohormones and carbohydrates, whereas ethylene may counteract ABA signaling. The regulation of senescence processes triggered by concerted action of phytohormones and glucose signaling may be an essential component of this mechanism.
The LPA3 Receptor: Regulation and Activation of Signaling Pathways
Authors: Solís Karina Helivier , Romero-Ávila M. Teresa , Guzmán-Silva Alejandro , García-Sáinz J. Adolfo
Published: June 2021

The lysophosphatidic acid 3 receptor (LPA3) participates in different physiological actions and in the pathogenesis of many diseases through the activation of different signal pathways. Knowledge of the regulation of the function of the LPA3 receptor is a crucial element for defining its roles in health and disease. This review describes what is known about the signaling pathways activated in terms of its various actions. Next, we review knowledge on the structure of the LPA3 receptor, the domains found, and the roles that the latter might play in ligand recognition, signaling, and cellular localization. Currently, there is some information on the action of LPA3 in different cells and whole organisms, but very little is known about the regulation of its function. Areas in which there is a gap in our knowledge are indicated in order to further stimulate experimental work on this receptor and on other members of the LPA receptor family. We are convinced that knowledge on how this receptor is activated, the signaling pathways employed and how the receptor internalization and desensitization are controlled will help design new therapeutic interventions for treating diseases in which the LPA3 receptor is implicated.
Different Expressions of HIF-1α and Metabolism in Brain and Major Visceral Organs of Acute Hypoxic Mice
Authors: Xu Lu , Song Hua , Qiu Qi , Jiang Ting , Ge Pingyun , Su Zaiji , Ma Wenhui , Zhang Ran , Huang Caihua , Li Shanhua , Lin Donghai , Zhang Jiaxing
Published: June 2021

Hypoxia is associated with clinical diseases. Extreme hypoxia leads to multiple organs failure. However, the different effects of hypoxia on brain and visceral organs still need to be clarified, and moreover, characteristics in vulnerable organs suffering from hypoxia remain elusive. In the present study, we first aimed to figure out the hypoxic sensitivity of organs. Adult male mice were exposed to 6% O2 or 8% O2 for 6 h. Control mice were raised under normoxic conditions. In vivo and in vitro imaging of anti-HIF-1α-NMs-cy5.5 nanocomposites showed that the expression level of hypoxia-inducible factor (HIF-1α) was the highest in the liver, followed by kidney and brain. HIF-1α was detected in the hepatocytes of liver, distal convoluted tubules of kidney and neurons of cerebral cortex. The liver, kidney and brain showed distinct metabolic profiles but an identical change in glutamate. Compared with kidney and brain, the liver had more characteristic metabolites and more disturbed metabolic pathways related to glutaminolysis and glycolysis. The level of O-phosphocholine, GTP, NAD and aspartate were upregulated in hypoxic mice brain, which displayed significant positive correlations with the locomotor activity in control mice, but not in hypoxic mice with impaired locomotor activities. Taken together, the liver, kidney and brain are the three main organs of the body that are strongly respond to acute hypoxia, and the liver exhibited the highest hypoxic sensitivity. The metabolic disorders appear to underlie the physiological function changes.
Studies of Conformational Changes of Tubulin Induced by Interaction with Kinesin Using Atomistic Molecular Dynamics Simulations
Authors: Shi Xiao-Xuan , Wang Peng-Ye , Chen Hong , Xie Ping
Published: June 2021

The transition between strong and weak interactions of the kinesin head with the microtubule, which is regulated by the change of the nucleotide state of the head, is indispensable for the processive motion of the kinesin molecular motor on the microtubule. Here, using all-atom molecular dynamics simulations, the interactions between the kinesin head and tubulin are studied on the basis of the available high-resolution structural data. We found that the strong interaction can induce rapid large conformational changes of the tubulin, whereas the weak interaction cannot. Furthermore, we found that the large conformational changes of the tubulin have a significant effect on the interaction of the tubulin with the head in the weak-microtubule-binding ADP state. The calculated binding energy of the ADP-bound head to the tubulin with the large conformational changes is only about half that of the tubulin without the conformational changes.
Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy
Authors: Dzurendová Simona , Shapaval Volha , Tafintseva Valeria , Kohler Achim , Byrtusová Dana , Szotkowski Martin , Márová Ivana , Zimmermann Boris
Published: June 2021

Oleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy for screening and process monitoring of filamentous fungi in biotechnology. Six Mucoromycota strains were cultivated in microbioreactors under six growth conditions (three phosphate concentrations in the presence and absence of calcium). FT-Raman and FT-infrared (FTIR) spectroscopic data was assessed in respect to reference analyses of lipids, phosphorus, and carotenoids by using principal component analysis (PCA), multiblock or consensus PCA, partial least square regression (PLSR), and analysis of spectral variation due to different design factors by an ANOVA model. All main chemical biomass constituents were detected by FT-Raman spectroscopy, including lipids, proteins, cell wall carbohydrates, and polyphosphates, and carotenoids. FT-Raman spectra clearly show the effect of growth conditions on fungal biomass. PLSR models with high coefficients of determination (0.83–0.94) and low error (approximately 8%) for quantitative determination of total lipids, phosphates, and carotenoids were established. FT-Raman spectroscopy showed great potential for chemical analysis of biomass of oleaginous filamentous fungi. The study demonstrates that FT-Raman and FTIR spectroscopies provide complementary information on main fungal biomass constituents.
The Role of ABC Transporters in Lipid Metabolism and the Comorbid Course of Chronic Obstructive Pulmonary Disease and Atherosclerosis
Authors: Kotlyarov Stanislav , Kotlyarova Anna
Published: June 2021

Chronic obstructive pulmonary disease (COPD) ranks among the leading causes of morbidity and mortality worldwide. COPD rarely occurs in isolation and is often combined with various diseases. It is considered that systemic inflammation underlies the comorbid course of COPD. The data obtained in recent years have shown the importance of violations of the cross-links of lipid metabolism and the immune response, which are links in the pathogenesis of both COPD and atherosclerosis. The role of lipid metabolism disorders in the pathogenesis of the comorbid course of COPD and atherosclerosis and the participation of ATP-binding cassette (ABC) transporters in these processes is discussed in this article. It is known that about 20 representatives of a large family of ABC transporters provide lipid homeostasis of cells by moving lipids inside the cell and in its plasma membrane, as well as removing lipids from the cell. It was shown that some representatives of the ABC-transporter family are involved in various links of the pathogenesis of COPD and atherosclerosis, which can determine their comorbid course.
Exogenous Hydrogen Sulfide Plays an Important Role by Regulating Autophagy in Diabetic-Related Diseases
Authors: Lv Shuangyu , Liu Huiyang , Wang Honggang
Published: June 2021

Autophagy is a vital cell mechanism which plays an important role in many physiological processes including clearing long-lived, accumulated and misfolded proteins, removing damaged organelles and regulating growth and aging. Autophagy also participates in a variety of biological functions, such as development, cell differentiation, resistance to pathogens and nutritional hunger. Recently, autophagy has been reported to be involved in diabetes, but the mechanism is not fully understood. Hydrogen sulfide (H2S) is a colorless, water-soluble, flammable gas with the typical odor of rotten eggs, which has been known as a highly toxic gas for many years. However, it has been reported recently that H2S, together with nitric oxide and carbon monoxide, is an important gas signal transduction molecule. H2S has been reported to play a protective role in many diabetes-related diseases, but the mechanism is not fully clear. Recent studies indicate that H2S plays an important role by regulating autophagy in many diseases including cancer, tissue fibrosis diseases and glycometabolic diseases; however, the related mechanism has not been fully studied. In this review, we summarize recent research on the role of H2S in regulating autophagy in diabetic-related diseases to provide references for future related research.
eNAMPT Is Localised to Areas of Cartilage Damage in Patients with Hip Osteoarthritis and Promotes Cartilage Catabolism and Inflammation
Authors: Philp Ashleigh M. , Butterworth Sam , Davis Edward T. , Jones Simon W.
Published: June 2021

Obesity increases the risk of hip osteoarthritis (OA). Recent studies have shown that adipokine extracellular nicotinamide phosphoribosyltransferase (eNAMPT or visfatin) induces the production of IL-6 and matrix metalloproteases (MMPs) in chondrocytes, suggesting it may promote articular cartilage degradation. However, neither the functional effects of extracellular visfatin on human articular cartilage tissue, nor its expression in the joint of hip OA patients of varying BMI, have been reported. Hip OA joint tissues were collected from patients undergoing joint replacement surgery. Cartilage explants were stimulated with recombinant human visfatin. Pro-inflammatory cytokines and MMPs were measured by ELISA and Luminex. Localisation of visfatin expression in cartilage tissue was determined by immunohistochemistry. Cartilage matrix degradation was determined by quantifying proteoglycan release. Expression of visfatin was elevated in the synovial tissue of hip OA patients who were obese, and was co-localised with MMP-13 in areas of cartilage damage. Visfatin promoted the degradation of hip OA cartilage proteoglycan and induced the production of pro-inflammatory cytokines (IL-6, MCP-1, CCL20, and CCL4) and MMPs. The elevated expression of visfatin in the obese hip OA joint, and its functional effects on hip cartilage tissue, suggests it plays a central role in the loss of cartilage integrity in obese patients with hip OA.
Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii
Authors: Yan Ying , Kobayashi Yukino , Huang Cong , Liu Bo , Qian Wanqiang , Wan Fanghao , Schetelig Marc F.
Published: June 2021

The spotted-wing Drosophila (Drosophila suzukii Matsumura) is native to eastern Asia, but has become a global threat to fruit production. In recent years, CRISPR/Cas9 targeting was established in this species allowing for functional genomic and genetic control studies. Here, we report the generation and characterization of Cas9-expressing strains of D. suzukii. Five independent transgenic lines were generated using a piggyBac construct containing the EGFP fluorescent marker gene and the Cas9 gene under the control of the D. melanogaster heat shock protein 70 promoter and 3’UTR. Heat-shock (HS) treated embryos were analyzed by reverse transcriptase PCR, revealing strong heat inducibility of the transgenic Cas9 expression. By injecting gRNA targeting EGFP into one selected line, 50.0% of G0 flies showed mosaic loss-of-fluorescence phenotype, and 45.5% of G0 flies produced G1 mutants without HS. Such somatic and germline mutagenesis rates were increased to 95.4% and 85.7%, respectively, by applying a HS. Parental flies receiving HS resulted in high inheritance of the mutation (92%) in their progeny. Additionally, targeting the endogenous gene yellow led to the lack of pigmentation and male lethality. We discuss the potential use of these efficient and temperature-dependent Cas9-expressing strains for the genetic studies in D. suzukii.
Membrane Interactions Accelerate the Self-Aggregation of Huntingtin Exon 1 Fragments in a Polyglutamine Length-Dependent Manner
Authors: Marquette Arnaud , Aisenbrey Christopher , Bechinger Burkhard
Published: June 2021

The accumulation of aggregated protein is a typical hallmark of many human neurodegenerative disorders, including polyglutamine-related diseases such as chorea Huntington. Misfolding of the amyloidogenic proteins gives rise to self-assembled complexes and fibres. The huntingtin protein is characterised by a segment of consecutive glutamines which, when exceeding ~ 37 residues, results in the occurrence of the disease. Furthermore, it has also been demonstrated that the 17-residue amino-terminal domain of the protein (htt17), located upstream of this polyglutamine tract, strongly correlates with aggregate formation and pathology. Here, we demonstrate that membrane interactions strongly accelerate the oligomerisation and β-amyloid fibril formation of htt17-polyglutamine segments. By using a combination of biophysical approaches, the kinetics of fibre formation is investigated and found to be strongly dependent on the presence of lipids, the length of the polyQ expansion, and the polypeptide-to-lipid ratio. Finally, the implications for therapeutic approaches are discussed.
Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
Authors: Gaweł Agata M. , Ratajczak Maciej , Gajda Ewa , Grzanka Małgorzata , Paziewska Agnieszka , Cieślicka Marta , Kulecka Maria , Oczko-Wojciechowska Małgorzata , Godlewska Marlena
Published: June 2021

Background: Thyroid carcinoma (TC) is the most common endocrine system malignancy, and papillary thyroid carcinoma (PTC) accounts for >80% of all TC cases. Nevertheless, PTC pathogenesis is still not fully understood. The aim of the study was to elucidate the role of the FRMD5 protein in the regulation of biological pathways associated with the development of PTC. We imply that the presence of certain genetic aberrations (e.g., BRAF V600E mutation) is associated with the activity of FRMD5. Methods: The studies were conducted on TPC1 and BCPAP (BRAF V600E) model PTC-derived cells. Transfection with siRNA was used to deplete the expression of FRMD5. The mRNA expression and protein yield were evaluated using RT-qPCR and Western blot techniques. Proliferation, migration, invasiveness, adhesion, spheroid formation, and survival tests were performed. RNA sequencing and phospho-kinase proteome profiling were used to assess signaling pathways associated with the FRMD5 expressional status. Results: The obtained data indicate that the expression of FRMD5 is significantly enhanced in BRAF V600E tumor specimens and cells. It was observed that a drop in intracellular yield of FRMD5 results in significant alternations in the migration, invasiveness, adhesion, and spheroid formation potential of PTC-derived cells. Importantly, significant divergences in the effect of FRMD5 depletion in both BRAF-wt and BRAF-mutated PTC cells were observed. It was also found that knockdown of FRMD5 significantly alters the expression of multidrug resistant genes. Conclusions: This is the first report highlighting the importance of the FRMD5 protein in the biology of PTCs. The results suggest that the FRMD5 protein can play an important role in controlling the metastatic potential and multidrug resistance of thyroid tumor cells.