bluuebunny/crossref_metadata_2025 huggingface dataset:
Iron Released after Cryo-Thermal Therapy Induced M1 Macrophage Polarization, Promoting the Differentiation of CD4+ T Cells into CTLs
Authors: Wang Shicheng , Cheng Man , Peng Peng , Lou Yue , Zhang Aili , Liu Ping
Published: June 2021

Macrophages play critical roles in both innate and adaptive immunity and are known for their high plasticity in response to various external signals. Macrophages are involved in regulating systematic iron homeostasis and they sequester iron by phagocytotic activity, which triggers M1 macrophage polarization and typically exerts antitumor effects. We previously developed a novel cryo-thermal therapy that can induce the mass release of tumor antigens and damage-associated molecular patterns (DAMPs), promoting M1 macrophage polarization. However, that study did not examine whether iron released after cryo-thermal therapy induced M1 macrophage polarization; this question still needed to be addressed. We hypothesized that cryo-thermal therapy would cause the release of a large quantity of iron to augment M1 macrophage polarization due to the disruption of tumor cells and blood vessels, which would further enhance antitumor immunity. In this study, we investigated iron released in primary tumors, the level of iron in splenic macrophages after cryo-thermal therapy and the effect of iron on macrophage polarization and CD4+ T cell differentiation in metastatic 4T1 murine mammary carcinoma. We found that a large amount of iron was released after cryo-thermal therapy and could be taken up by splenic macrophages, which further promoted M1 macrophage polarization by inhibiting ERK phosphorylation. Moreover, iron promoted DC maturation, which was possibly mediated by iron-induced M1 macrophages. In addition, iron-induced M1 macrophages and mature DCs promoted the differentiation of CD4+ T cells into the CD4 cytolytic T lymphocytes (CTL) subset and inhibited differentiation into Th2 and Th17 cells. This study explains the role of iron in cryo-thermal therapy-induced antitumor immunity from a new perspective.
Identification of Nrl1 Domains Responsible for Interactions with RNA-Processing Factors and Regulation of Nrl1 Function by Phosphorylation
Authors: Mikolaskova Barbora , Jurcik Matus , Cipakova Ingrid , Selicky Tomas , Jurcik Jan , Polakova Silvia Bagelova , Stupenova Erika , Dudas Andrej , Sivakova Barbara , Bellova Jana , Barath Peter , Aronica Lucia , Gregan Juraj , Cipak Lubos
Published: June 2021

Pre-mRNA splicing is a key process in the regulation of gene expression. In the fission yeast Schizosaccharomyces pombe, Nrl1 regulates splicing and expression of several genes and non-coding RNAs, and also suppresses the accumulation of R-loops. Here, we report analysis of interactions between Nrl1 and selected RNA-processing proteins and regulation of Nrl1 function by phosphorylation. Bacterial two-hybrid system (BACTH) assays revealed that the N-terminal region of Nrl1 is important for the interaction with ATP-dependent RNA helicase Mtl1 while the C-terminal region of Nrl1 is important for interactions with spliceosome components Ctr1, Ntr2, and Syf3. Consistent with this result, tandem affinity purification showed that Mtl1, but not Ctr1, Ntr2, or Syf3, co-purifies with the N-terminal region of Nrl1. Interestingly, mass-spectrometry analysis revealed that in addition to previously identified phosphorylation sites, Nrl1 is also phosphorylated on serines 86 and 112, and that Nrl1-TAP co-purifies with Cka1, the catalytic subunit of casein kinase 2. In vitro assay showed that Cka1 can phosphorylate bacterially expressed Nrl1 fragments. An analysis of non-phosphorylatable nrl1 mutants revealed defects in gene expression and splicing consistent with the notion that phosphorylation is an important regulator of Nrl1 function. Taken together, our results provide insights into two mechanisms that are involved in the regulation of the spliceosome-associated factor Nrl1, namely domain-specific interactions between Nrl1 and RNA-processing proteins and post-translational modification of Nrl1 by phosphorylation.
Bioactive Lipid O-cyclic phytosphingosine-1-phosphate Promotes Differentiation of Human Embryonic Stem Cells into Cardiomyocytes via ALK3/BMPR Signaling
Authors: Jang Ji-Hye , Kim Min-Seong , Antao Ainsley Mike , Jo Won-Jun , Kim Hyung-Joon , Kim Su-Jin , Choi Myeong-Jun , Ramakrishna Suresh , Kim Kye-Seong
Published: June 2021

Adult human cardiomyocytes have an extremely limited proliferative capacity, which poses a great barrier to regenerative medicine and research. Human embryonic stem cells (hESCs) have been proposed as an alternative source to generate large numbers of clinical grade cardiomyocytes (CMs) that can have potential therapeutic applications to treat cardiac diseases. Previous studies have shown that bioactive lipids are involved in diverse cellular responses including cardiogenesis. In this study, we explored the novel function of the chemically synthesized bioactive lipid O-cyclic phytosphingosine-1-phosphate (cP1P) as an inducer of cardiac differentiation. Here, we identified cP1P as a novel factor that significantly enhances the differentiation potential of hESCs into cardiomyocytes. Treatment with cP1P augments the beating colony number and contracting area of CMs. Furthermore, we elucidated the molecular mechanism of cP1P regulating SMAD1/5/8 signaling via the ALK3/BMP receptor cascade during cardiac differentiation. Our result provides a new insight for cP1P usage to improve the quality of CM differentiation for regenerative therapies.
Differences between Human and Mouse IgM Fc Receptor (FcµR)
Authors: Kubagawa Hiromi , Skopnik Christopher M. , Al-Qaisi Khlowd , Calvert Rosaleen A. , Honjo Kazuhito , Kubagawa Yoshiki , Teuber Ruth , Aliabadi Pedram Mahmoudi , Enghard Philipp , Radbruch Andreas , Sutton Brian J.
Published: June 2021

Both non-immune “natural” and antigen-induced “immune” IgM are important for protection against pathogens and for regulation of immune responses to self-antigens. Since the bona fide IgM Fc receptor (FcµR) was identified in humans by a functional cloning strategy in 2009, the roles of FcµR in these IgM effector functions have begun to be explored. In this short essay, we describe the differences between human and mouse FcµRs in terms of their identification processes, cellular distributions and ligand binding activities with emphasis on our recent findings from the mutational analysis of human FcµR. We have identified at least three sites of human FcµR, i.e., Asn66 in the CDR2, Lys79 to Arg83 in the DE loop and Asn109 in the CDR3, responsible for its constitutive IgM-ligand binding. Results of computational structural modeling analysis are consistent with these mutational data and a model of the ligand binding, Ig-like domain of human FcµR is proposed. Serendipitously, substitution of Glu41 and Met42 in the CDR1 of human FcµR with mouse equivalents Gln and Leu, either single or more prominently in combination, enhances both the receptor expression and IgM binding. These findings would help in the future development of preventive and therapeutic interventions targeting FcµR.
Diagnostic Value of Salivary miRNA in Head and Neck Squamous Cell Cancer: Systematic Review and Meta-Analysis
Authors: Kang Jeong-Wook , Eun Young-Gyu , Lee Young-Chan
Published: June 2021

Several studies have highlighted the diagnostic potential of salivary microRNA (miRNA) in head and neck squamous cell cancer (HNSCC). The purpose of this meta-analysis was to summarize published studies and evaluate the diagnostic accuracy of salivary miRNA in HNSCC detection. In this meta-analysis, we systematically searched PubMed, EMBASE, and Cochrane Library databases for studies on miRNA and HNSCC diagnosis. Pooled sensitivity, specificity, and diagnostic odds ratio (DOR) with a summary receiver-operating characteristic curve were calculated using a bivariate random-effect meta-analysis model. Furthermore, subgroup analyses were conducted to explore the main sources of heterogeneity. Seventeen studies from ten articles, including 23 miRNA and a total of 759 subjects, were included in this meta-analysis. The pooled sensitivity and specificity of salivary miRNA in the diagnosis of HNSCC were 0.697 (95% CI: 0.644–0.744) and 0.868 (95% CI: 0.811–0.910), respectively. The overall area under the curve was 0.803 with a DOR of 12.915 (95% CI: 9.512–17.534). Salivary miRNAs are a promising non-invasive diagnostic biomarker with moderate accuracy for HNSCC. These results must be verified by large-scale prospective studies.
Efficacy of Telemedicine and Telemonitoring in At-Home Monitoring of Patients with COVID-19
Authors: Casariego-Vales Emilio , Blanco-López Rosa , Rosón-Calvo Benigno , Suárez-Gil Roi , Santos-Guerra Fernando , Dobao-Feijoo María , Ares-Rico Ramón , Bal-Alvaredo Mercedes ,   on behalf of the TELEA-COVID Lugo Comanagement Team
Published: June 2021

Aim: this work aims to assess if telemedicine and telemonitoring are clinically useful and safe for at-home monitoring of Coronavirus disease 2019 (COVID-19) patients. Methods: This is a retrospective cohort study of all patients diagnosed with COVID-19 in Galicia (Northwestern Spain) between 26 December 2020 and 15 February 2021. The structured, proactive monitoring via telemonitoring (TELEA) of patients considered to be high-risk in the Lugo, A Mariña, and Monforte Healthcare Area (ASLAM) was evaluated compared to other models in the remaining healthcare areas of Galicia. Results: Of the 47,053 COVID-19 patients, 4384 (9.3%) were in ASLAM. Of them, 1187 (27.1%) were monitored via TELEA, and the rest (3197 in ASLAM and 42,669 in the rest of Galicia) were monitored via other methods. Patients monitored in ASLAM via TELEA were older, consulted in the emergency department less frequently (p = 0.05), were hospitalized less frequently (p < 0.01), had shorter hospital stays (p < 0.0001), and had a lower mortality rate in their first hospitalization (p = 0.03). No at-home life-threatening emergencies were recorded. Conclusions: these data suggest that, for COVID-19 patients, a care model involving proactive at-home monitoring with telemedicine and telemonitoring is associated with reduced pressure on hospital services and a lower mortality rate.
Influence of the Halloysite Nanotube (HNT) Addition on Selected Mechanical and Biological Properties of Thermoplastic Polyurethane
Authors: Mrówka Maciej , Szymiczek Małgorzata , Machoczek Tomasz , Pawlyta Mirosława
Published: June 2021

Halloysite nanotube (HNT) additions to the thermoplastic polyurethane (TPU) system were thoroughly evaluated in this study. The resultant composites have been designed for future personalized intervertebral disc implant applications, which requires additional technology to obtain the appropriate geometry unique to each patient. These requirements can be fulfilled using 3D printing. In this work, a technology was developed to produce filaments for fused deposition modeling (FDM). Nanocomposites were prepared using variable HNT content (1, 2, and 3 wt.%). The nanostructure of the resultant composites was confirmed using scanning transmission electron microscopy (STEM). Mechanical tests were used to measure the tensile modulus, stress, and elongation the composites and TPU matrix. Nanocomposites with 2% HNT content were able to withstand 26% increased stress and 50% increased elongation compared to pure TPU before fracturing in addition to a 13% reduction in the friction coefficient. A MTT cytotoxicity assay confirmed the cytotoxicity of all tested materials against human epidermal keratinocyte cells (HaCaT).
Strength Capacity and Failure Mode of Shear Connectors Suitable for Composite Cold Formed Steel Beams: Numerical Study
Authors: Elsawaf Sherif A. , Bamaga Saleh O.
Published: June 2021

In this paper, the findings of numerical modeling of the composite action between normal concrete and Cold-Formed Steel (CFS) beams are presented. To obtain comprehensive structural behavior, the numerical model was designed using 3-D brick components. The simulation results were correlated to the experimental results of eight push tests, using three types of innovative shear connectors in addition to standard headed stud shear connectors, with two different thicknesses of a CFS channel beam. The proposed numerical model was found to be capable of simulating the failure mode of the push test as well as the behavior of shear connectors in order to provide composite action between the cold-formed steel beam and concrete using the concrete damaged plasticity model.
Longitudinal Deformation of Deep Shield Tunnels Caused by Upper Load Reduction
Authors: Zheng Jinlei , He Shaohui , Li Yiming , He Jiaxin , He Jihua
Published: June 2021

Above-crossing excavations may cause uplift damages on existing shield tunnels. Therefore, to accurately calculate the deformation of shield tunnels is very necessary for geotechnical engineers. At present, the single-sided elastic foundation beam model is usually used in longitudinal deformation calculations for shield tunnels, which overestimates the uplift of deep shield tunnels. Because of the existence of the ground arch, deep shield tunnels are subjected to two-sided foundation reaction forces. Therefore, this paper proposes a partial missing double-sided elastic foundation beam model and the related fourth-order partial differential equations. In this model, the shield tunnel is subjected to double Winkler foundation springs and is simply considered a Euler–Bernoulli beam. A two-stage analysis method is used to solve the problem. First, the vertical unloading stress due to the above-crossing tunnelling at the tunnel location is calculated through Mindlin’s solution. Second, the deformation response of the beam subjected to an unloading stress is calculated by the finite difference method. Two engineering cases are used to verify the research. The results indicate that the proposed model is more accurate than traditional models in predicting the maximum uplift value, which is basically consistent with the observations. Due to the existence of segment staggering, the longitudinal influence range of the calculation by two models is larger than the actual measurement.
Central Composite Design Optimisation in Single Point Incremental Forming of Truncated Cones from Commercially Pure Titanium Grade 2 Sheet Metals
Authors: Szpunar Marcin , Ostrowski Robert , Trzepieciński Tomasz , Kaščák Ľuboš
Published: June 2021

Single point incremental forming (SPIF) is an emerging process that is well-known to be suited for fabrication in small series production. The aim of this paper was to determine the optimal input parameters of the process in order to minimise the maximum of both the axial and the in-plane components of the forming force achieved during SPIF and the surface roughness of the internal surface of truncated-cone drawpieces. Grade 2 pure titanium sheets with a thickness of 0.4 mm were used as the test material. The central composite design and response surface method was used to determine the number of experiments required to study the responses through building a second-order quadratic model. Two directions of rotation of the forming tool were also considered. The input parameters were spindle speed, tool feed rate, and step size. The mathematical relations were defined using the response surfaces to predict the surface roughness of the drawpieces and the components of the forming force. It was found that feed rate has an insignificant role in both axial and in-plane forming forces, but step size is a major factor affecting axial and radial forming forces. However, step size directly affects the surface roughness on the inner surfaces of the drawpieces. Overall, the spindle speed −579 rpm (clockwise direction), tool feed 2000 mm/min, and step size 0.5 mm assure a minimisation of both force components and the surface roughness of drawpieces.
InfPolyn, a Nonparametric Bayesian Characterization for Composition-Dependent Interdiffusion Coefficients
Authors: Xing Wei W. , Cheng Ming , Cheng Kaiming , Zhang Wei , Wang Peng
Published: June 2021

Composition-dependent interdiffusion coefficients are key parameters in many physical processes. However, finding such coefficients for a system with few components is challenging due to the underdetermination of the governing diffusion equations, the lack of data in practice, and the unknown parametric form of the interdiffusion coefficients. In this work, we propose InfPolyn, Infinite Polynomial, a novel statistical framework to characterize the component-dependent interdiffusion coefficients. Our model is a generalization of the commonly used polynomial fitting method with extended model capacity and flexibility and it is combined with the numerical inversion-based Boltzmann–Matano method for the interdiffusion coefficient estimations. We assess InfPolyn on ternary and quaternary systems with predefined polynomial, exponential, and sinusoidal interdiffusion coefficients. The experiments show that InfPolyn outperforms the competitors, the SOTA numerical inversion-based Boltzmann–Matano methods, with a large margin in terms of relative error (10× more accurate). Its performance is also consistent and stable, whereas the number of samples required remains small.
Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota
Authors: Catarino Marcelo D. , Marçal Catarina , Bonifácio-Lopes Teresa , Campos Débora , Mateus Nuno , Silva Artur M. S. , Pintado Maria Manuela , Cardoso Susana M.
Published: June 2021

Recent studies indicate that plant polyphenols could be pointed as potential prebiotic candidates since they may interact with the gut microbiota, stimulating its growth and the production of metabolites. However, little is known about the fate of brown seaweeds’ phlorotannins during their passage throughout the gastrointestinal tract. This work aimed to evaluate the stability and bioaccessibility of Fucus vesiculosus phlorotannins after being submitted to a simulated digestive process, as well as their possible modulatory effects on gut microbiota and short-chain fatty acids production following a fermentation procedure using fecal inoculates to mimic the conditions of the large intestine. The stability of phlorotannins throughout the gastrointestinal tract was reduced, with a bioaccessibility index between 2 and 14%. Moreover, slight alterations in the growth of certain commensal bacteria were noticed, with Enterococcus spp. being the most enhanced group. Likewise, F. vesiculosus phlorotannins displayed striking capacity to enhance the levels of propionate and butyrate, which are two important short-chain fatty acids known for their role in intestinal homeostasis. In summary, this work provides valuable information regarding the behavior of F. vesiculosus phlorotannins along the gastrointestinal tract, presenting clear evidence that these compounds can positively contribute to the maintenance of a healthy gastrointestinal condition.
Technique for Measuring Power across High Resistive Load of Triboelectric Energy Harvester
Authors: Jayasvasti Subhawat , Thainiramit Panu , Yingyong Phonexai , Isarakorn Don
Published: June 2021

This paper proposed a more-accurate-than-conventional measurement technique for determining electrical power across exceptionally high-impedance of triboelectric energy harvester (TEH). The key idea of this proposed technique was to measure the voltage across an introduced, parallelly-connected resistor divider to the oscilloscope instead of the voltage across the harvester. An experiment was set up to verify the measurement accuracy performance of this technique against the ideal theoretical values. The maximum percentage error found was only 2.30%, while the conventional measurement technique could not be used to measure voltage across high impedance TEH at all because the readings were not accurate, i.e., the measurement error would be at least over 10%. Therefore, we concluded that this proposed technique should always be used instead of the conventional measurement technique for power measurement of any TEH. A suggestion that we would like to offer to researchers investigating or developing a TEH is that, in using our measurement technique, a good starting point for a load to probe resistance ratio is 1:10, a ratio that worked well for our TEH test bench that we developed.
Role of Calixarene in Chemotherapy Delivery Strategies
Authors: Basilotta Rossella , Mannino Deborah , Filippone Alessia , Casili Giovanna , Prestifilippo Angela , Colarossi Lorenzo , Raciti Gabriele , Esposito Emanuela , Campolo Michela
Published: June 2021

Since cancer is a multifactorial disease with a high mortality rate, the study of new therapeutic strategies is one of the main objectives in modern research. Numerous chemotherapeutic agents, although widely used, have the disadvantage of being not very soluble in water or selective towards cancerous cells, with consequent side effects. Therefore, in recent years, a greater interest has emerged in innovative drug delivery systems (DDSs) such as calixarene, a third-generation supramolecular compound. Calixarene and its water-soluble derivatives show good biocompatibility and have low cytotoxicity. Thanks to their chemical–physical characteristics, calixarenes can be easily functionalized, and by itself can encapsulate host molecules forming nanostructures capable of releasing drugs in a controlled way. The encapsulation of anticancer drugs in a calixarene derivate improves their bioavailability and efficacy. Thus, the use of calixarenes as carriers of anticancer drugs could reduce their side effects and increase their affinity towards the target. This review summarizes the numerous research advances regarding the development of calixarene nanoparticles capable of encapsulating various anticancer drugs.
Photosensitizing Antivirals
Authors: Mariewskaya Kseniya A. , Tyurin Anton P. , Chistov Alexey A. , Korshun Vladimir A. , Alferova Vera A. , Ustinov Alexey V.
Published: June 2021

Antiviral action of various photosensitizers is already summarized in several comprehensive reviews, and various mechanisms have been proposed for it. However, a critical consideration of the matter of the area is complicated, since the exact mechanisms are very difficult to explore and clarify, and most publications are of an empirical and “phenomenological” nature, reporting a dependence of the antiviral action on illumination, or a correlation of activity with the photophysical properties of the substances. Of particular interest is substance-assisted photogeneration of highly reactive singlet oxygen (1O2). The damaging action of 1O2 on the lipids of the viral envelope can probably lead to a loss of the ability of the lipid bilayer of enveloped viruses to fuse with the lipid membrane of the host cell. Thus, lipid bilayer-affine 1O2 photosensitizers have prospects as broad-spectrum antivirals against enveloped viruses. In this short review, we want to point out the main types of antiviral photosensitizers with potential affinity to the lipid bilayer and summarize the data on new compounds over the past three years. Further understanding of the data in the field will spur a targeted search for substances with antiviral activity against enveloped viruses among photosensitizers able to bind to the lipid membranes.
Electroreduction of Bi(III) Ions at a Cyclically Renewable Liquid Silver Amalgam Film Electrode in the Presence of Methionine
Authors: Nosal-Wiercińska Agnieszka , Martyna Marlena , Mirčeski Valentin , Skrzypek Sławomira
Published: June 2021

The catalytic influence of methionine (Mt) on the electroreduction of Bi(III) ions on the novel, cyclically renewable liquid silver amalgam film electrode (R–AgLAFE) in a non-complexing electrolyte solution was examined. The presence of methionine leads to a multistep reaction mechanism, where the transfer of the first electron is the rate limiting step, which is the subject of catalytic augmentation. The catalytic activity of methionine is a consequence of its ability to remove water molecules from the bismuth ion coordination sphere, as well as to form active complexes on the electrode surface, facilitating the electron transfer process.
Protein Denaturation Through the Use of Magnetic Molecularly Imprinted Polymer Nanoparticles
Authors: Boitard Charlotte , Michel Aude , Ménager Christine , Griffete Nébéwia
Published: June 2021

The inhibition of the protein function for therapeutic applications remains challenging despite progress these past years. While the targeting application of molecularly imprinted polymer are in their infancy, no use was ever made of their magnetic hyperthermia properties to damage proteins when they are coupled to magnetic nanoparticles. Therefore, we have developed a facile and effective method to synthesize magnetic molecularly imprinted polymer nanoparticles using the green fluorescent protein (GFP) as the template, a bulk imprinting of proteins combined with a grafting approach onto maghemite nanoparticles. The hybrid material exhibits very high adsorption capacities and very strong affinity constants towards GFP. We show that the heat generated locally upon alternative magnetic field is responsible of the decrease of fluorescence intensity.
Applications of Nanofluids
Authors: Sheremet Mikhail A.
Published: June 2021

Nanofluids as a combination of base fluid and a low concentration of nano-sized particles of metal or metal oxides are used in different fields of human activity, including engineering devices in power and chemical engineering, medicine, electronics, and others [...]
Extra Virgin Olive Oil Prevents the Age-Related Shifts of the Distribution of HDL Subclasses and Improves Their Functionality
Authors: Otrante Alyann , Trigui Amal , Walha Roua , Berrougui Hicham , Fulop Tamas , Khalil Abdelouahed
Published: June 2021

High-density lipoproteins (HDL) maintain cholesterol homeostasis through the role they play in regulating reverse cholesterol transport (RCT), a process by which excess cholesterol is transported back to the liver for elimination. However, RCT can be altered in the presence of cardiovascular risk factors, such as aging, which contributes to the increase in the incidence of cardiovascular diseases (CVD). The present study was aimed at investigating the effect of extra virgin olive oil (EVOO) intake on the cholesterol efflux capacity (CEC) of HDL, and to elucidate on the mechanisms by which EVOO intake improves the anti-atherogenic activity of HDL. A total of 84 healthy women and men were enrolled and were distributed, according to age, into two groups: 27 young (31.81 ± 6.79 years) and 57 elderly (70.72 ± 5.6 years) subjects. The subjects in both groups were given 25 mL/d of extra virgin olive oil (EVOO) for 12 weeks. CEC was measured using J774 macrophages radiolabeled with tritiated cholesterol ((3H) cholesterol). HDL subclass distributions were analyzed using the Quantimetrix Lipoprint® system. The HDL from the elderly subjects exhibited a lower level of CEC, at 11.12% (p < 0.0001), than the HDL from the young subjects. The CEC of the elderly subjects returned to normal levels following 12 weeks of EVOO intake. An analysis of the distribution of HDL subclasses showed that HDL from the elderly subjects were composed of lower levels of large HDL (L-HDL) (p < 0.03) and higher levels of small HDL (S-HDL) (p < 0.002) compared to HDL from the young subjects. A multiple linear regression analysis revealed a positive correlation between CEC and L-HDL levels (r = 0.35 and p < 0.001) as well as an inverse correlation between CEC and S-HDL levels (r = −0.27 and p < 0.01). This correlation remained significant even when several variables, including age, sex, and BMI as well as low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and glucose levels (β = 0.28, p < 0.002, and β = 0.24, p = 0.01) were accounted for. Consuming EVOO for 12 weeks modulated the age-related difference in the distribution of HDL subclasses by reducing the level of S-HDL and increasing the level of intermediate-HDL/large-HDL (I-HDL/L-HDL) in the elderly subjects. The age-related alteration of the CEC of HDL was due, in part, to an alteration in the distribution of HDL subclasses. A diet enriched in EVOO improved the functionality of HDL through an increase in I-HDL/L-HDL and a decrease in S-HDL.
Impact of Dietary Trajectories on Obesity and Dental Caries in Preschool Children: Findings from the Healthy Smiles Healthy Kids Study
Authors: Manohar Narendar , Hayen Andrew , Scott Jane A. , Do Loc G. , Bhole Sameer , Arora Amit
Published: June 2021

This study examines the impact of longitudinal dietary trajectories on obesity and early childhood caries (ECC) in preschool children in Australia. Mother–infant dyads from the Healthy Smiles Healthy Kids study were interviewed at 4 and 8 months, and 1, 2, and 3 years of age. Children underwent anthropometric and oral health assessments between 3 and 4 years of age. Multivariable logistic regression and negative binomial regression analysis were performed for the prevalence of overweight and obesity, and the number of tooth surfaces with dental caries, respectively. The intake of core, discretionary, and sugary foods showed distinct quadratic (n = 3) trajectories with age. The prevalence of overweight or obesity was 10% (n = 72) and that of early childhood caries (ECC) was 33% (mean decayed, missing, and filled tooth surfaces (dmfs) score: 1.96). Children with the highest trajectories of discretionary foods intake were more likely to be overweight or obese (adjusted OR: 2.51, 95 %CI: 1.16–5.42). Continued breastfeeding beyond 12 months was associated with higher dmfs scores (adjusted IRR: 2.17, 95 %CI: 1.27–3.73). Highest socioeconomic disadvantage was the most significant determinant for overweight or obesity (adjusted OR: 2.86, 95 %CI: 1.11–7.34) and ECC (adjusted IRR: 2.71, 95 %CI: 1.48–4.97). Targeted health promotion interventions should be designed to prevent the incidence of two highly prevalent conditions in preschool children.
Breakfast Frequency and Composition in a Group of Polish Children Aged 7–10 Years
Authors: Kawalec Anna , Pawlas Krystyna
Published: June 2021

Breakfast is considered one of the crucial elements of a healthy diet. Most studies evaluate breakfast consumption with the risk of obesity and other health effects. Less attention is paid to the evaluation of breakfast composition and patterns. Thus, this study aimed to describe the most frequently observed breakfast patterns and to assess breakfast composition and quality in a group of Polish early school-age children. The cross-sectional survey study was conducted in school years 2017/2018 and 2018/2019. Information regarding breakfast was obtained with the use of an original paper-based weekly observation diary, and breakfast quality was assessed with a special scoring designed to be used together with the diary. In total, 223 schoolchildren of the second and third grades participated in the study, and 200 diaries were analyzed. More than ¾ of the participants consumed breakfast every day. Nearly 68% of meals were classified as well balanced, but only 16.5% of children eat a well-balanced breakfast every day. The number of children who usually (≥5 times per week) eat a sandwich for breakfast was 94 (47%), and that of those who habitually eat cereal and milk or porridge was 29 (14.5%). Only 7% of children consumed fruit or vegetables for breakfast daily, and 26.5% never eat fruit or vegetables for breakfast. Concluding, most children eat breakfast regularly, but the meal composition and quality might be improved.
Self-Care Instruments to Measure Nutrition Practices in Children and Parents: Psychometric Analysis
Authors: Pawloski Lisa R. , Moore Jean B. , Treffinger Patricia , Baghi Heibatollah , Gaffney Kathleen , Jaimovich Sonia , Campos Cecilia , Curtin Kevin M.
Published: June 2021

The purposes of this study were to evaluate the psychometric properties of English and Spanish instruments that measure the nutrition behavior and practices of children and their parents. Orem’s self-care deficit nursing theory was used in this methodological study. A convenience sample of 333 children and 262 mothers participated from two schools in Washington, D.C. and two schools in Santiago, Chile. Principal component analysis indicated three component per instrument corresponding to Orem’s Theory of operations demonstrating construct validity of the instrument. The study findings showed evidence for validity and reliability of the English and Spanish versions and indicated that the instruments appropriately represented Orem’s operations. The results have implications for the development of health behavior measurement instruments that are valid, reliable, designed for children, culturally appropriate, and efficient. Measuring the nutrition behavior of children and parents is critical for determining the effectiveness of nutrition intervention programs. Furthermore, instruments are needed so that researchers can compare corresponding child and parent behaviors or compare behaviors across cultures.
Novel Receptor Tyrosine Kinase Pathway Inhibitors for Targeted Radionuclide Therapy of Glioblastoma
Authors: Bolcaen Julie , Nair Shankari , Driver Cathryn , Boshomane Tebatso , Ebenhan Thomas , Vandevoorde Charlot
Published: June 2021

Glioblastoma (GB) remains the most fatal brain tumor characterized by a high infiltration rate and treatment resistance. Overexpression and/or mutation of receptor tyrosine kinases is common in GB, which subsequently leads to the activation of many downstream pathways that have a critical impact on tumor progression and therapy resistance. Therefore, receptor tyrosine kinase inhibitors (RTKIs) have been investigated to improve the dismal prognosis of GB in an effort to evolve into a personalized targeted therapy strategy with a better treatment outcome. Numerous RTKIs have been approved in the clinic and several radiopharmaceuticals are part of (pre)clinical trials as a non-invasive method to identify patients who could benefit from RTKI. The latter opens up the scope for theranostic applications. In this review, the present status of RTKIs for the treatment, nuclear imaging and targeted radionuclide therapy of GB is presented. The focus will be on seven tyrosine kinase receptors, based on their central role in GB: EGFR, VEGFR, MET, PDGFR, FGFR, Eph receptor and IGF1R. Finally, by way of analyzing structural and physiological characteristics of the TKIs with promising clinical trial results, four small molecule RTKIs were selected based on their potential to become new therapeutic GB radiopharmaceuticals.
TRK Inhibitors: Tissue-Agnostic Anti-Cancer Drugs
Authors: Han Sun-Young
Published: June 2021

Recently, two tropomycin receptor kinase (Trk) inhibitors, larotrectinib and entrectinib, have been approved for Trk fusion-positive cancer patients. Clinical trials for larotrectinib and entrectinib were performed with patients selected based on the presence of Trk fusion, regardless of cancer type. This unique approach, called tissue-agnostic development, expedited the process of Trk inhibitor development. In the present review, the development processes of larotrectinib and entrectinib have been described, along with discussion on other Trk inhibitors currently in clinical trials. The on-target effects of Trk inhibitors in Trk signaling exhibit adverse effects on the central nervous system, such as withdrawal pain, weight gain, and dizziness. A next generation sequencing-based method has been approved for companion diagnostics of larotrectinib, which can detect various types of Trk fusions in tumor samples. With the adoption of the tissue-agnostic approach, the development of Trk inhibitors has been accelerated.
Morphological Analysis of Several Bamboo Species with Potential Structural Applications
Authors: Elejoste Asier , Arevalillo Alfonso , Gabilondo Nagore , Butron Amaia , Peña-Rodriguez Cristina
Published: June 2021

Bamboo constitutes a family of plants that are very promising and interesting as renewable materials for both large and small structure construction. To be used as an alternative to traditional materials; the understanding of its morphology and mechanical behavior is of crucial importance. As the distribution of fibers and vascular bundles differs for each type of bamboo; several bamboo types have been characterized: Phyllostachys aurea (PA), Arundinaria amabilis (AA) and Dendrocalamus strictus (DS). Morphological analysis has been performed by optical (OM) and scanning electron microscopy (SEM). Differences in density; surface morphology and wall thickness have been found. In fact; PA and AA have shown a great morphological regularity; while DS presents the greatest thickness; to the point that it can be considered full culm. The plant’s own ducts constitute a very important factor for future impregnations and the optimization of mechanical properties for structure construction.
In-Line Rheo-Optical Investigation of the Dispersion of Organoclay in a Polymer Matrix during Twin-Screw Compounding
Authors: Teixeira Paulo F. , Covas José A. , Hilliou Loïc
Published: June 2021

The dispersion mechanisms in a clay-based polymer nanocomposite (CPNC) during twin-screw extrusion are studied by in-situ rheo-optical techniques, which relate the CPNC morphology with its viscosity. This methodology avoids the problems associated with post extrusion structural rearrangement. The polydimethylsiloxane (PDMS) matrix, which can be processed at ambient and low temperatures, is used to bypass any issues associated with thermal degradation. Local heating in the first part of the extruder allows testing of the usefulness of low matrix viscosity to enhance polymer intercalation before applying larger stresses for clay dispersion. The comparison of clay particle sizes measured in line with models for the kinetics of particle dispersion indicates that larger screw speeds promote the break-up of clay particles, whereas smaller screw speeds favor the erosion of the clay tactoids. Thus, different levels of clay dispersion are generated, which do not simply relate to a progressively better PDMS intercalation and higher clay exfoliation as screw speed is increased. Reducing the PDMS viscosity in the first mixing zone of the screw facilitates dispersion at lower screw speeds, but a complex interplay between stresses and residence times at larger screw speeds is observed. More importantly, the results underline that the use of larger stresses is inefficient per se in dispersing clay if sufficient time is not given for PDMS to intercalate the clay galleries and thus facilitate tactoid disruption or erosion.
Characterization and Ex Vivo Application of Indocyanine Green Chitosan Patches in Dura Mater Laser Bonding
Authors: Rossi Francesca , Magni Giada , Colasanti Roberto , Banchelli Martina , Iacoangeli Maurizio , Carrassi Erika , Aiudi Denis , Di Rienzo Alessandro , Giannoni Luca , Pieri Laura , Dallari Stefano , Pini Roberto , Matteini Paolo
Published: June 2021

Dura mater repair represents a final and crucial step in neurosurgery: an inadequate dural reconstruction determines dreadful consequences that significantly increase morbidity and mortality rates. Different dural substitutes have been used with suboptimal results. To overcome this issue, in previous studies, we proposed a laser-based approach to the bonding of porcine dura mater, evidencing the feasibility of the laser-assisted procedure. In this work, we present the optimization of this approach in ex vivo experiments performed on porcine dura mater. An 810-nm continuous-wave AlGaAs (Aluminium Gallium Arsenide) diode laser was used for welding Indocyanine Green-loaded patches (ICG patches) to the dura. The ICG-loaded patches were fabricated using chitosan, a resistant, pliable and stable in the physiological environment biopolymer; moreover, their absorption peak was very close to the laser emission wavelength. Histology, thermal imaging and leak pressure tests were used to evaluate the bonding effect. We demonstrated that the application of 3 watts (W), pulsed mode (Ton 30 ms, Toff 3.5 ms) laser light induces optimal welding of the ICG patch to the dura mater, ensuring an average fluid leakage pressure of 216 ± 105 mmHg, falling within the range of physiological parameters. This study demonstrated that the thermal effect is limited and spatially confined and that the laser bonding procedure can be used to close the dura mater. Our results showed the effectiveness of this approach and encourage further experiments in in vivo models.
A Methodology for Evaluating the Progression of Damage in a Glass Fibre Reinforced Polymer Laminate Subjected to Vertical Weight Drop Impacts
Authors: Townsend Patrick , Suárez-Bermejo Juan C. , Rodríguez-Ortíz Álvaro
Published: June 2021

This study describes a methodology that allows evaluating the behavior of a glass fibre reinforced polymer (GFRP) laminate impacted by a vertical weight drop, analyzing the damage that occurred inside. The purpose of the designers was, by means of characterization tests of the curing processes, evaluation of the cohesion of a particular laminate, application of vertical tests by weight drops and with the use of the readings of an accelerometer in a single direction, know the trend of how intralaminar breaks in the matrix and interlaminar breaks between layers occur. It is proposed to establish the behavior of the laminate before the tests by analyzing curing times, for after the tests by observations with penetrating fluorescent inks. This allows researchers to know the response of the laminate to the loads imposed on the applied structure. For the tests, prepreg material cured outside the autoclave in an oven was used and qualitative quantification of the damage by observing sections of the tested material infiltrated with penetrating fluorescent ink exposed to ultraviolet light.
Sequential Laser–Mechanical Drilling of Thick Carbon Fibre Reinforced Polymer Composites (CFRP) for Industrial Applications
Authors: Ahmad Sobri Sharizal , Heinemann Robert , Whitehead David
Published: June 2021

Carbon fibre reinforced polymer composites (CFRPs) can be costly to manufacture, but they are typically used anywhere a high strength-to-weight ratio and a high steadiness (rigidity) are needed in many industrial applications, particularly in aerospace. Drilling composites with a laser tends to be a feasible method since one of the composite phases is often in the form of a polymer, and polymers in general have a very high absorption coefficient for infrared radiation. The feasibility of sequential laser–mechanical drilling for a thick CFRP is discussed in this article. A 1 kW fibre laser was chosen as a pre-drilling instrument (or initial stage), and mechanical drilling was the final step. The sequential drilling method dropped the overall thrust and torque by an average of 61%, which greatly increased the productivity and reduced the mechanical stress on the cutting tool while also increasing the lifespan of the bit. The sequential drilling (i.e., laser 8 mm and mechanical 8 mm) for both drill bits (i.e., 2- and 3-flute uncoated tungsten carbide) and the laser pre-drilling techniques has demonstrated the highest delamination factor (SFDSR) ratios. A new laser–mechanical sequence drilling technique is thus established, assessed, and tested when thick CFRP composites are drilled.
Biocomposites of Low-Density Polyethylene Plus Wood Flour or Flax Straw: Biodegradation Kinetics across Three Environments
Authors: Zykova Anna K. , Pantyukhov Petr V. , Mastalygina Elena E. , Chaverri-Ramos Christian , Nikolaeva Svetlana G. , Saavedra-Arias Jose J. , Popov Anatoly A. , Wortman Sam E. , Poletto Matheus
Published: June 2021

The purpose of this study was to assess the potential for biocomposite films to biodegrade in diverse climatic environments. Biocomposite films based on polyethylene and 30 wt.% of two lignocellulosic fillers (wood flour or flax straw) of different size fractions were prepared and studied. The developed composite films were characterized by satisfactory mechanical properties that allows the use of these materials for various applications. The biodegradability was evaluated in soil across three environments: laboratory conditions, an open field in Russia, and an open field in Costa Rica. All the samples lost weight and tensile strength during biodegradation tests, which was associated with the physicochemical degradation of both the natural filler and the polymer matrix. The spectral density of the band at 1463 cm−1 related to CH2-groups in polyethylene chains decreased in the process of soil burial, which is evidence of polymer chain breakage with formation of CH3 end groups. The degradation rate of most biocomposites after 20 months of the soil assays was greatest in Costa Rica (20.8–30.9%), followed by laboratory conditions (16.0–23.3%), and lowest in Russia (13.2–22.0%). The biocomposites with flax straw were more prone to biodegradation than those with wood flour, which can be explained by the chemical composition of fillers and the shape of filler particles. As the size fraction of filler particles increased, the biodegradation rate increased. Large particles had higher bioavailability than small spherical ones, encapsulated by a polymer. The prepared biocomposites have potential as an ecofriendly replacement for traditional polyolefins, especially in warmer climates.
Hygrothermal Aging Characteristics of Silicone-Modified Aging-Resistant Epoxy Resin Insulating Material
Authors: Wang Yongqiang , Zeng Zhuo , Gao Meng , Huang Ziye
Published: June 2021

To study the improvement effect of silicone materials on the hygrothermal resistance of epoxy resin and the aging mechanism of silicone-modified insulation materials under hygrothermal conditions, diphenylsilanediol was added to epoxy resin as a modifier in various quantities to synthesize silicone-modified epoxy resin, and a hygrothermal aging test was carried out. Water sorption, surface contact angles and dielectric properties of the insulation material were measured, and scanning electron microscope (SEM), Fourier-transform infrared spectrometry (FT-IR) and frequency domain spectroscopy (FDS) were used to analyze the results. The results showed that under 10 wt%, the silicone-modified insulation materials exhibited lower absorption rate and better dielectric properties, including lower dissipation factors and lower dielectric constant during the hygrothermal aging process, while epoxy resin modified with excessive silicone material tend to show worse dielectric performance. Closer analysis found that diphenylsilanediol decreases the size of the cracks within the material during hygrothermal aging, indicating that cracks generated during the hygrothermal aging process may be the reason for the worse dielectric performance, and diphenylsilanediol improves the hygrothermal aging resistance mainly by slowing down the generation and growth rate of cracks. FT-IR results confirmed the existence of hydrolysis and found that the rate of hydrolysis does not change with the content of diphenylsilanediol. FDS results also indicated that modified materials contain less dipoles after hygrothermal aging.