bluuebunny/crossref_metadata_2025 huggingface dataset:
Rhetoric Strategies in the Presidents` Speeches: Ukrainian versus English
Authors: Nasakina Svitlana V. , Kolisnichenko Natalia M. , Rohalska-Yakubova Inna I. , Chepelyuk Nataliya I.
Published: November 2020

The article focuses on the prominent stylistic devices of the language, gives their definitions, and examines the effectiveness of their use in the political speeches of the Prime Minister of the United Kingdom of Great Britain and Northern Ireland, the Presidents of Ukraine, and the President of the United States of America. It studies the rhetorical strategies that help the speakers to achieve the desired goal effectively, and add persuasiveness to the addresses. The article aims to reveal the similar tendencies in rhetorical speeches used by the political leaders. The research method, which includes two stages, has been presented. The first stage consisted of material collection. The second stage consisted of two sub-stages of the study and included the description, and the systematization of the obtained data. The significance of the research lies in the fact that the similar traits in political speeches in different countries have been defined. The further studying of the political speeches of the political elites can enable to deepen the knowledge about rhetorical strategies in the sociolinguistic aspect.
Does wine innovation research require ageing? A bibliometric review
Authors: Porto-Gómez Igone , Larreina Mikel , Gaviria-de-la-Puerta José
Published: November 2020

Although wine is an old issue, wine business research and particularly innovation in the wine sector seem to be relatively young areas in the literature. With its production limited to certain regions but its consumption being more widespread, wine plays a relevant role in the economic development of some rural European regions. Considering this strong influence in certain European countries, the purpose of this study is to provide a comprehensive assessment of innovation in wine-related articles published in scholarly journals between 1998 and 2019 to describe how this field of research has evolved. This article combines a systematic literature review process with a bibliometric review. The use of both methodologies makes a universal, neutral and reliable contribution, reducing the possible bias of traditional literature reviews. The main advantage of the employment of a bibliometric methodology is the speed with which we can analyse a large number of documents to obtain the key issues identified in the literature. A relevant finding in our research is that sustainable innovation is emerging as a distinct type of innovation, related to those involving procedural and organizational changes. The results also exhibit that a large fraction of wine business literature is regionally based, which makes sense given the characteristics of the industry.
Masal Kitaplarında Yer Alan Korku ve Şiddet Ögeleri
Authors: GÜZELKÜÇÜK Şüra , TÜRKYILMAZ Mustafa
Published: November 2020

Bu araştırma, masallarda yer alan korku ve şiddet unsurlarını tespit edip değerlendirmek amacıyla gerçekleştirilmiştir. Eserlerin incelenmesinde nitel araştırma yöntemlerinden doküman analizi yöntemi kullanılmıştır. Eserlerin korku ve şiddet yönünden incelenmesinde hareket noktası olarak “cümle” kabul edilmiştir. Elde edilen verilerin çözümlenmesinde Türkçe eğitimi alan uzmanlarının görüşlerinden de yararlanılmış ve içerik analizi yöntemi kullanılmıştır.
Araştırmanın veri kaynağını, ikisi dünya edebiyatından, dördü Türk edebiyatından toplam altı masal kitabı ve bu masal kitaplarında yer alan 480 masal oluşturmaktadır.
Araştırmanın sonucunda, masal kitaplarında korku ve şiddet unsurları tespit edilmiştir. Kitaplarda yer alan şiddet türleri sınıflandırılmış yapılan değerlendirmede; yerli ve yabancı masal kitaplarında en fazla karşılaşılan şiddet türlerinin sözel şiddet ve fiziksel şiddet olduğu bulunmuştur. Bu sıralamayı duygusal şiddet ve cinsel şiddet türlerinin takip ettiği görülmüştür. Araştırma sonucunda elde edilen verilere göre Türk edebiyatında yer alan masallardaki şiddet unsurlarının, dünya edebiyatında yer alan masallardan daha fazla olduğu belirlenmiştir. Masal kitaplarındaki şiddet ve korku unsurlarının kullanım sıklığı karşılaştırıldığında; korku içeren unsurların, şiddet içeren unsurlardan az olduğu sonucuna ulaşılmıştır. İçeriğinde en fazla korku unsuru bulunan masalın Andersen Masalları olduğu, en az korku unsuru bulunan masalın ise, Aisopos Masalları olduğu tespit edilmiştir.
Pengembangan Media Bantu Latihan Bertahan Pada Bola Voli Tahun 2019
Authors: Ramlan Sahputera Sagala  , Dicky Edwar Daulay
Published: November 2020

This study aims to develop defense training aids in volleyball 2019. The population in this study was 10 PBV volleyball athletes. Pem.Panjang, 10 PBV athletes. Sukaraja and 10 PBV athletes. 50 in 2019. The results of small group tests involving 20 athletes, 10 PBV Pem.Panjang volleyball athletes and 10 PBV 50 athletes showed that the development of defense stone training tools in volleyball 2019 can be concluded that these defense latiam aids meet the criteria to be continued in trials large groups with conditions after repairs due to the percentage of questionnaire statements between 45% -74%. The results of a large group test involving 30 athletes, 10 PBV Pem.Panjang volleyball athletes, 10 PBV 50 athletes and 10 PBV Sukaraja athletes that the development of defense training aids in the 2019 volleyball game can be concluded that defense training aids meet the criteria for use in helps athlete defense training because the percentage of questionnaire statements is between 78% -85%. So it can be concluded that this defense training aid can be used to train defense.
METODOLOGIA DA APRENDIZAGEM BASEADA EM PROJETOS COMO FERRAMENTA PARA IDENTIFICAR PROBLEMAS DE GESTÃO: UM ESTUDO DE CASO
Authors: Pablo Eduardo Nikolais T. Bonifácio da Silva
Published: November 2020

Este trabalho tem como objetivo investigar se é possível identificar problemas de gestãoempresarial com a metodologia Aprendizagem Baseada em Projetos (ABPj). A pesquisaenquadra-se como descritiva e qualitativa, em que a coleta de dados foi realizada por meio dosrelatórios elaborados pelos 20 alunos da turma do mestrado do Programa de Pós-Graduação emCiências Contábeis da Universidade Federal do Rio Grande do Norte no decorrer do projeto.Através dos resultados foi possível identificar que os principais problemas da empresa refletema falta de conhecimentos gerenciais por parte dos seus gestores e que é necessário enxergar osdepartamentos da empresa como um todo. Sendo assim, conclui-se que caso a metodologia daABPj seja bem empregada pode realmente ajudar a identificar os problemas de uma empresa eajudar ao gestor a solucionar tais adversidades para alcançar o sucesso organizacional.
Efecto del riego con agua residual tratada sobre la calidad microbiológica del suelo y pasto King Grass
Authors:   Universidad Popular del Cesar, González-Fragozo Harold E. , Zabaleta-Solano Carolina ,   Universidad Popular del Cesar, Devia-González Jessica ,   Universidad Popular del Cesar, Moya-Salinas Yulieth ,   Universidad Popular del Cesar, Afanador-Rico Otilia ,   Universidad Popular del Cesar
Published: November 2020

La contaminación del agua utilizada en el mundo y la falta de plantas de tratamiento altamente eficientes plantean problemas potenciales para la salud pública y el medio ambiente, por lo que los países buscan métodos confiables, inocuos y eficaces en función de los costos, para depurar las aguas residuales. Por otro lado, el sector agropecuario ha tomado auge, dada la necesidad de implementación de técnicas limpias, que sean amigables con el medio ambiente. El riego con aguas residuales en la agricultura, se ha vuelto común en regiones áridas y semiáridas, debido a la baja disponibilidad de agua, pero su uso requiere de un monitoreo cuidadoso de parámetros de higiene. El objetivo de este trabajo fue analizar el efecto del riego, con aguas residuales tratadas, sobre la contaminación microbiológica del suelo y el pasto King Grass, además de la producción de materia seca del forraje. Se evaluaron tres tratamientos: tratamiento T1: sin riesgo (SR), T2: agua subterránea (AS) y T3: agua residual tratada (ART). Se hicieron análisis fisicoquímicos y microbiológicos al agua, suelo y pasto. La contaminación microbiológica en el suelo regado con agua residual tratada no presentó diferencias significativas (p>0,05) frente a los suelos donde se aplicó riego con agua subterránea, así como tampoco se presentaron diferencias estadísticas significativas entre variables microbiológicas en pasto. Se concluyó, que la fuente de contaminación microbiológica de los pastos y el suelo no está relacionada únicamente con la carga microbiana presente en las aguas residuales, sino que se puede deber, entre otros factores, a las escorrentías y al uso de aguas subterráneas contaminadas para el riego.
Editorial - Journal of Greek Archaeology Volume 5
Authors: Bintliff John
Published: November 2020

Volume 5 is perhaps the richest and most diverse volume we have been able to offer you readers. We have kept to our brief to cover all the major periods of Greek Archaeology in a literal sense, with articles from the Neolithic through Greco-Roman times and the Middle Ages and up to the 19th century AD. Geographically we range from Sicily through the Aegean to Turkey.
Late Antique and Medieval Landscapes of the Nemea Valley, Southern Greece
Authors: Cloke Christian , Athanassopoulos Effie
Published: November 2020

In the last 40 years regional survey projects and excavations in the Aegean region have produced a rich record of Late Antique and Medieval land use and settlement. They have provided information about rural sites and villages, subjects that are poorly covered by historical sources. In this paper we examine the contribution of surface survey, along with information derived from excavation, to the understanding of these periods. The case study comes from the region of Nemea, in southern Greece, which has been the focus of excavations by the University of California-Berkeley and an intensive survey as part of the Nemea Valley Archaeological Project (NVAP) (Figure 1a-b).
Archaeological survey and understanding the rural landscape in Byzantine Greece: some specific examples
Authors: Gregory Timothy E. , Tzortzopoulou-Gregory Lita
Published: November 2020

In a review of the second volume of the Nemea Valley Archaeological Project Series, a significant contribution in the use of archaeological survey data to explore complex sociopolitical phenomena in the development of the medieval settlement pattern of the northeastern Peloponnese, Michael Given states the following: ‘This volume publishes valuable and interesting material on a period for which, even after some four decades of intensive archaeological survey, there is a striking lack of fully published survey results. This in itself is a major contribution, particularly when combined with the high quality of the original survey, the care with which the pottery and site descriptions are presented, the extensive historical background and the superb illustrations. The volume itself does not present a fully integrated archaeological, historical, and landscape analysis of this important material. Its real value, however, is that it allows us all to do our own integration and analysis.’ Our paper seeks to follow on from this final statement, and as such it wishes first to investigate some general considerations about survey archaeology in the broader world of the eastern Mediterranean, and to then examine more specifically the ways in which they are appropriate to the medieval period in ongoing research. Drawing from our own survey work in northern Kythera and the eastern Korinthia, we will attempt to demonstrate the limitations of an overarching diachronic approach to archaeological survey, and the need for continuous re-evaluation of a survey’s research design and methodology to the specific demands dictated by each different archaeological/historical period.
Syracuse and its environs from c. 6000 to 650 BC: the prehistoric and Greek origins of the city
Authors: Leighton Robert
Published: November 2020

Scholars, as well as modern residents, might well agree with Cicero that Syracuse was ‘the richest and fairest’ of Greek cities in Sicily, where its pre-eminence is well attested by historical and archaeological evidence. The nature and form of the settlements which preceded Greek colonization, however, are much harder to evaluate and have never been the subject of a unified analysis or synthesis. Sealed under buildings and layers accumulated throughout nearly 3000 years of urban development, the remains of prehistoric and early Greek Syracuse are largely invisible today, although they have been uncovered periodically by archaeologists over the last century or more. This article offers a chronologically extended view of the city’s origins, which situates the island promontory of Ortygia within a regional settlement network encompassing the natural bay area (or great harbour) and its hinterland. Starting in the Neolithic, but with the focus on later periods, I assess the initial expansion of Greek Syracuse with reference to pre-existing spatial relationships and cultural adaptations, which began to take shape during the Bronze and Iron Ages.
Technology for increasing safety of operation of pit-run and motor roads and prevention of dusting of tailings dumps by treatment them by natural bischofite water solutions
Authors: Gurin A. A. , Shapovalov V. A. , Lyashenko V. I.
Published: November 2020

The ore mined in open pits, the depth of which often exceeds 200 m, is taken out of them as a ruie by dump trucks having capacity of up to 300 t. Provision of safety of pit-run motor transport and uninterrupted open pit operation, particuiariy in wintertime is an important task. Pecuiiarities of pit-run motor roads running presented. Existing methods of preventing winter siipperiness at motor roads considered, their possibilities and drawbacks highlighted. A technology elaborated to prevent the winter slipperiness and dusting of pit-run motor roads by treatment them by natural bischofite (MgCl2•6H2O) water solutions (RPB). The RPB has a density of 1250 kg/m3, freezing temperature -35 °С and is attributed to the substance of 4th class of danger. Dependence of rocks mass freezing temperature on the content of water RPB in it determined. Testing results of industrial technology of prevention winter slipperiness and dusting of motor roads in the open pits of Krivbas, Ukraine presented. The technology includes preliminary preventive treatment of the roads by a water solution of natural bischofite. Recommendations on its concentration, necessary for roads surface treatment were given, depending on the air temperature at various open pit depth and time of treatment commencement after receiving weather forecast. It was recommended to treat the pit-run motor roads by reagent of water RPB on the surface at air temperature down to -3 °С at the depth of more than 100 m - lower -3 °С, at the depth of more than 200 m - lower -5 °С. Recommendations elaborated to prevent dusting of motor roads, dumps and tailings dumps of mining and concentration plants by application natural bischofite water solutions, which effectively fasten the dusting surfaces preventing blowing-off the dust from them.
Possibilities of using functional biologically active organosilicon compounds in veterinary practice
Authors: Barkova A. , Shurmanova E. , Honina Tat'yana , Mil'shteyn I.
Published: November 2020

Abstract. Purpose. Evaluation of the possibility of using silicon, silicon-zinc, silicon-zinc organoboron compounds and compositions based on them for the treatment and prevention of inflammatory and non-inflammatory diseases in agricultural and small domestic animals. Method. There was carried out a clinical trial of various medicinal compositions with a base in the form of organic compounds of silicon, zinc, boron, both containing antimicrobial components and environmentally friendly agents. For this purpose, groups of animals were formed on the principle of analogs. The multiplicity and administration of drugs depended on the studied pathology. Results. The obtained results showed a high efficiency of the studied compositions, in particular, in the treatment of postpartum diseases of the uterus of an inflammatory nature, the effectiveness of the application was 95.5 %, with conservative treatment of the retention of the placenta – 100 %. Also, these funds are highly effective for the treatment of hyperkeratosis of the udder nipples, without changing the technology of milking. On average, the effectiveness of the compositions reached 86.1 % when the funds were used for 7 days. The study of an antimicrobial composition based on organosilicon glycerohydrogel in the treatment of purulent-necrotic lesions in the distal part of the extremities in cows can reduce the frequency of procedures and the duration of treatment by 6 days compared to animals in the control group. Scientific novelty. New functional biologically active pharmacological agents based on silicon-, silicon-zinc-, silicon-zinc boron glycerohydrogels have been developed and clinically tested for the treatment of cows with postpartum uterine diseases, in particular, postpartum endometritis and retention of the placenta, hyperkeratosis of the udder nipples, purulent-necrotic diseases of the distal also necrotic skin lesions of small domestic animals.
Inclusão do aluno surdo na Prática Pedagógica
Authors: Cruz Delzuita Santana , Santos Eliete Correia dos
Published: November 2020

Esta pesquisa aborda a inclusão do aluno surdo na prática pedagógica da escola pública. O objetivo geral é analisar como a escola trabalha a inclusão desse aluno e se os profissionais que atuam estão preparados para recebê-los. Os objetivos específicos são identificar as dificuldades encontradas pelos alunos surdos e pelos professores na sua atuação. Quais os mecanismos que eles desenvolvem no sentido de vencer estas dificuldades e compreender a importância de o aluno surdo estar em contato com outros surdos e ouvintes para o desenvolvimento social. Esta investigação foi de cunho descritivo exploratório, em uma abordagem qualitativa. Para colher a informação necessária optou-se pela observação seguida de questionário que foi enviado para 27 profissionais que lidam diretamente com alunos surdos, dentre eles direção, coordenação pedagógica, professores do ensino regular, professores e intérprete de LIBRAS, alunos surdos, alunos ouvintes e pais de alunos ouvintes. Desses, somente 25 aceitaram participar. Os dados evidenciaram que as escolas em análise não dispõem de Intérprete de LIBRAS, e que os profissionais que atuam com os alunos surdos não têm conhecimento da Língua de Sinais para dar suporte a esses alunos em sala de aula. Nesta pesquisa evidencia que 8% dos profissionais possuem Pós-graduação em LIBRAS. 20% possuem Pós-graduação em Letras, 20% possuem Pós-graduação em Pedagogia, 8% possuem Pós-graduação em História. 4% possuem o Magistério com curso Tradutor/Intérprete em LIBRAS, 4% possuem o Ensino Médio completo, 24% estão cursando o Ensino Fundamental Anos Finais e 8% possui o Ensino Fundamental Anos iniciais. Embora haja alguns profissionais com formação, entre os participantes, ainda não estão voltados, de fato, para a sua atuação como intérprete uma vez que, 88% dos entrevistados não possuem curso básico na área de LIBRAS. Conclui-se que o papel do intérprete é relevante no contexto educacional inclusivo. Nota-se que esse profissional participa diretamente do avanço dos alunos com surdez no processo de Ensino/aprendizagem.
Adesão ao tratamento da tuberculose no Amapá: Um quinquênio de análise epidemiológica
Authors: Santana Jorge Lucas Alves , Magrini Amanda Thais , Ribeiro Lucas Almeida , Rigelli Roberto Heck , Araújo Maria Helena Mendonça de
Published: November 2020

A tuberculose é uma doença causada pela infecção do Mycobacterium tuberculosis de disseminação por meio de inalação de gotículas de aerossóis, acometendo em grande parte dos casos os pulmões, chamada nesses casos de tuberculose pulmonar, por isso todo paciente que apresenta sintomas respiratórios acima de três semanas, como tosse seca ou produtiva, é considerado suspeito, devendo iniciar a investigação da doença. Essa infecção é um problema de saúde pública de importância principal em países subdesenvolvidos ou em desenvolvimento, ligada a questões sociais e de higiene sanitária. O coeficiente de mortalidade é aproximadamente 2,25 por 100 mil habitantes no Brasil, o que resulta em cerca de 4500 óbitos por ano. O presente estudo tem por objetivo traçar o perfil epidemiológico quantitativamente de forma retrospectiva analisando a adesão ao tratamento por pacientes infectados pelo bacilo de Koch, no estado do Amapá, no recorte temporal de 2015 a 2019, utilizando dados do departamento de informática do Sistema Único de Saúde brasileiro (DATASUS) e correlacionar com informações atuais da literatura. Por meio disso, foram encontradas 1401 notificações de tuberculose no estado, durante esse quinquênio estudado, sendo estas mais recorrentes no sexo masculino e na faixa etária adulta. A taxa de abondo do tratamento mostrou-se similar entre homens e mulheres, com menores índices na faixa etária idosa. Dessa forma, a análise dos dados fornecidos pelo ministério da saúde sobre a temática nesta população mostra-se como uma forma de contribuir com a disseminação de informação sobre a situação epidemiológica amapaense da doença em questão, bem como, atentar os gestores de saúde acerca de medidas de prevenção e manejo mais eficazes no estado.
Pemanfaatan sumber-sumber internet dalam kegiatan membaca ekstensif di rumah
Authors: Nur Maulidia Rachmawati
Published: November 2020

This exploratory case study aims to capture a research participant’s learning experience in applying internet sources in extensive reading activities carried out at home, as a strategy that can be adopted to foster reading interest. The interview guide with open-ended questions was used as the instrument to obtain data about this experience. The findings reveal that the research participant had positive experiences and reflections on extensive reading activities supported by internet sources. This is because these internet-based reading sources are equipped by an attractive display, theme and type of texts, which were not previously obtained during the formal learning setting.

 

Abstrak

Penelitian ini bertujuan untuk memotret pengalaman belajar seorang anak dalam memanfaatkan sumber-sumber internet di kegiatan membaca ekstensif yang dilakukan di rumah, sebagai salah satu strategi yang bisa dimanfaatkan untuk menumbuhkan minat membaca. Studi kasus eksploratif digunakan sebagai desain penelitian dalam riset ini Panduan wawancara dengan pertanyaan terbuka digunakan sebagai instrumen untuk mendapatkan data mengenai pengalaman tersebut. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa partisipan riset memiliki pengalaman dan refleksi yang positif terhadap kegiatan membaca ekstensif yang didukung oleh sumber- sumber internet. Hal ini disebabkan karena sumber-sumber bacaan berbasis internet tersebut, yang didukung oleh tampilan dan jenis teks yang menarik, mampu memberi pengalaman dan wawasan yang baru bagi partisipan yang sebelumnya tidak didapatkan selama proses pembelajaran formal di sekolah.
A Sustainable Development Concept in the Light of the Kingdom of God
Authors: Matijević Dalia
Published: November 2020

In searching for answers about the actual relation between two apparently incompatible concepts: the Kingdom of God and the Sustainable Development (SD), we will try to put them side by side and use respective comparative findings, in order to see if they can work together and possibly benefit from each other. The aim is to better understand future perspectives that are opening for the church within the contemporary context. The two concepts considered in this research, the Kingdom of God and SD, are never supposed to be separated. Although coming from different sources, using different languages, and springing out from different socio-political contexts, they tackle the same set of universal issues; both are eagerly anticipated, their fulfilment is of utmost importance, they both call for profound personal and communal transformation, both are powerfully creative and innovative, and both have the capacity to mobilize communities, peoples, and resources. The intersections between the SD and the Kingdom of God create opportunities for the church to practice shalom and to nurture hesed in the world by encouraging inclusiveness, lobbing for social justice, carrying for the poor and marginalized, and extending love and compassion onto the whole of creation, without losing its genuine identity. Within the SD framework, the role of the church is to make sure that nobody is left behind. The church might be the best part of the SD practice by persistently insisting on the same universal set of Kingdom values no matter how circumstances are changing.
The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cell expansion
Authors: Astori Audrey , Matherat Gabriel , Munoz Isabelle , Gautier Emilie-Fleur , Surdez Didier , Zermati Yaël , Verdier Frédérique , Zaidi Sakina , Feuillet Vincent , Kadi Amir , Lauret Evelyne , Delattre Olivier , Lefèvre Carine , Fontenay Michaela , Ségal-Bendirdjian Evelyne , Dusanter-Fourt Isabelle , Bouscary Didier , Hermine Olivier , Mayeux Patrick , Pendino Frédéric
Published: November 2020

The gene CXXC5, encoding a retinoid-inducible nuclear factor (RINF), is located within a region at 5q31.2 commonly deleted in myelodysplastic syndrome and adult acute myeloid leukemia. RINF may act as an epigenetic regulator and has been proposed as a tumor suppressor in hematopoietic malignancies. However, functional studies in normal hematopoiesis are lacking, and its mechanism of action is unknown. Here, we evaluated the consequences of RINF silencing on cytokine-induced erythroid differentiation of human primary CD34+ progenitors. We found that RINF is expressed in immature erythroid cells and that RINF-knockdown accelerated erythropoietin-driven maturation, leading to a significant reduction (~45%) in the number of red blood cells, without affecting cell viability. The phenotype induced by RINF-silencing was dependent on tumor growth factor b (TGFb) and mediated by SMAD7, a TGFb-signaling inhibitor. RINF upregulates SMAD7 expression by direct binding to its promoter and we found a close correlation between RINF and SMAD7 mRNA levels both in CD34+ cells isolated from bone marrow of healthy donors and myelodysplastic syndrome patients with del(5q). Importantly, RINF knockdown attenuated SMAD7 expression in primary cells and ectopic SMAD7 expression was sufficient to prevent the RINF knockdown-dependent erythroid phenotype. Finally, RINF silencing affects 5’-hydroxymethylation of human erythroblasts, in agreement with its recently described role as a TET2-anchoring platform in mouse. Collectively, our data bring insight into how the epigenetic factor RINF, as a transcriptional regulator of SMAD7, may fine-tune cell sensitivity to TGFb superfamily cytokines and thus play an important role in both normal and pathological erythropoiesis.
The Effect of Cooperative Type Number Heads Together and Concept Mapping Learning Models on Student's Learning Activity and Student Learning Outcomes in Class V Science Study of SD Negeri 11 Rantau Selatan
Authors: Lehawati Lehawati , Sutopo Adi , Winahyu Sekar
Published: November 2020

One of the lessons that make students more active is cooperative learning. Cooperative learning is a way of learning in which students of all abilities work together in small groups to achieve goals. This model prioritizes student-centred learning and students can interact with their friends in solving problems. The cooperative model has several types, including the cooperative model, Number Head Together. Concept Mapping was first developed by Tony Buzan, a mathematician, psychologist and researcher. Concept Mapping helps students to improve learning outcomes. Sampling using purposive sampling technique. while the sample in the study consisted of 2 classes, namely class 5 a as the experimental class 1 for Testing the Effect of the Cooperative Learning Model type Number Head Together and Concept Mapping on Student Learning Activities and Outcomes, and Class V b as the experimental class 2. The Cooperative Learning Model Type Number Head Together and the Concept Mapping Learning Model have a significant effect on Learning Activeness and Student Learning Outcomes in Class V Science Subjects at SD Negeri 11 Rantau Selatan. However, when analysed on the aspect of a stronger influence on Activities and Learning Outcomes, based on the results of the above calculations, it is obtained data that the Concept Mapping Learning Model has a stronger effect on student learning activeness compared to learning outcomes in science subjects. The Number Head Together Learning Model has a stronger influence on the results than the student learning activity in science learning materials.
Differences of Basic School Students' Network Results in the Implementation of STAD Type Cooperative Learning Models Aided by Animation Media and Power Point Media in the Plant Subtemes of My Friends from Learning Activities
Authors: Suryani Arnida , Sudrajat Ajat , Sapriati Amalia
Published: November 2020

The purpose of this study was to determine the differences in student learning outcomes in terms of student learning activities through the implementation of cooperative learning model Student Team Achievement Divisions (STAD) assisted by animation media and cooperative learning model type student team achievement divisions (STAD) assisted by power point media. This research will be conducted at SD Negeri 11 Rantau Selatan in Class VI the 2019/2020 Academic Year. The sampling technique uses purposive sampling, where researchers can determine samples based on specific objectives. The researcher determined that the sample in the study consisted of 2 classes, namely class VI a as a class of cooperative learning model type STAD assisted by animation media as many as 33 people, and class VI as many as 33 people as a class of cooperative learning model type STAD assisted by Power Point media. The instruments used were the Observation Sheet and the Test / Question Sheet. Measurements were made by Reliability, Distinguishing Power Analysis, Descriptive Data Analysis, Prerequisite Test Analysis, Two Ways ANOVA Hypothesis Test Analysis. The results of the study There are differences in learning outcomes in the sub-theme of Tumbuhan Sahabatku between those taught with the Cooperative Learning Model Type Student Team Achievement Division with animation aided with the Cooperative Learning Model Type Student Team Achievement Division assisted by Media Power Point in Class VI SD Negeri 11 Rantau Selatan, There are differences in results study of students who have high learning activities in the sub-theme of Tumbuhan Sahabatku, and there is an interaction between learning models and learning activities in influencing student learning outcomes in the sub-theme of Tumbuhan Sahabatku.
Secagem de materiais cerâmicos argilosos com forma complexa: uma análise teórica usando o método da capacitância global
Authors: Lima Wanderson Magno Paiva Barbosa de , Silva Eduarda Holanda , Lima Elisiane Santana de , Gomez Ricardo Soares , Magalhães Hortência Luma Fernandes , Silva Stephane Katherine Barbosa Moura da , Porto Túlio Rafael Nascimento , Lima Antonio Gilson Barbosa de
Published: November 2020

A necessidade de investimento na melhoria de qualidade, produtividade, redução de custos e economia de energia de processo é uma preocupação crescente do setor ceramista. O processo de secagem consiste na remoção de umidade de um material através do aquecimento, envolvendo alto consumo de energia. Neste sentido, este trabalho tem como objetivo estudar teoricamente a secagem de sólidos cerâmicos vazados e com forma arbitrária utilizando uma modelagem matemática fenomenológica baseando-se no método da capacitância global. Resultados cinéticos de perda de massa e aquecimento do sólido vazado em diferentes condições operacionais são apresentados e analisados. Observou-se que a perda de umidade ocorre numa menor velocidade que o aquecimento do material, que o formato do sólido e sua relação área/volume afetam fortemente os fenômenos de transporte de calor e massa e que, dependendo da severidade, pode ocasionar danos na estrutura como deformações, trincas, empenamentos e perda do produto.
In silico Assessment of Pharmacological Profile of Low Molecular Weight Oligo-Hydroxyalkanoates
Authors: Roman Diana Larisa , Isvoran Adriana , Filip Mǎdǎlina , Ostafe Vasile , Zinn Manfred
Published: November 2020

Polyhydroxyalkanoates (PHAs) are a large class of polyesters that are biosynthesized by microorganisms at large molecular weights (Mw > 80 kDa) and have a great potential for medical applications because of their recognized biocompatibility. Among PHAs, poly(3-hydroxybutyrate), poly(4-hydroxybutyrate), poly(3-hydroxyvalerate), poly(4-hydroxyvalerate), and their copolymers are proposed to be used in biomedicine, but only poly(4-hydroxybutyrate) has been certified for medical application. Along with the hydrolysis of these polymers, low molecular weight oligomers are released typically. In this study, we have used a computational approach to assess the absorption, distribution, metabolism, and excretion (ADME)-Tox profiles of low molecular weight oligomers (≤32 units) consisting of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxyvalerate, 3-hydroxybutyrate-co-3-hydroxyvalerate, and the hypothetical PHA consisting of 4-hydroxybutyrate-co-4-hydroxyvalerate. According to our simulations, these oligomers do not show cardiotoxicity, hepatotoxicity, carcinogenicity or mutagenicity, and are neither substrates nor inhibitors of the cytochromes involved in the xenobiotic’s metabolism. They also do not affect the human organic cation transporter 2 (OCT2). However, they are considered to be inhibitors of the organic anion transporters OATP1B1, and OATP1B3. In addition, they may produce eye irritation, and corrosion, skin irritation and have a low antagonistic effect on the androgen receptor.
Dissection of Anti-tumor Activity of Histone Deacetylase Inhibitor SAHA in Nasopharyngeal Carcinoma Cells via Quantitative Phosphoproteomics
Authors: Huang Huichao , Fu Ying , Zhang Ye , Peng Fang , Lu Miaolong , Feng Yilu , Chen Lin , Chen Zhuchu , Li Maoyu , Chen Yongheng
Published: November 2020

Suberoylanilide hydroxamic acid (SAHA), a pan HDAC inhibitor, has been approved by the Food and Drug Administration (FDA) to treat cutaneous T cell lymphoma (CTCL). Nevertheless, the mechanisms underlying the therapeutic effects of SAHA on tumors are yet not fully understood. Protein phosphorylation is one of the most important means to regulate key biological processes (BPs), such as cell division, growth, migration, differentiation, and intercellular communication. Thus, investigation on the impacts of SAHA treatment on global cellular phosphorylation covering major signaling pathways deepens our understanding on its anti-tumor mechanisms. Here we comprehensively identified and quantified protein phosphorylation for the first time in nasopharyngeal carcinoma (NPC) cells upon SAHA treatment by combining tandem mass tags (TMTs)-based quantitative proteomics and titanium dioxide (TiO2)-based phosphopeptide enrichment. In total, 7,430 phosphorylation sites on 2,456 phosphoproteins were identified in the NPC cell line 5-8F, of which 1,176 phosphorylation sites on 528 phosphoproteins were significantly elevated upon SAHA treatment. Gene ontology (GO) analysis showed that SAHA influenced several BPs, including mRNA/DNA processing and cell cycle. Furthermore, signaling pathway analysis and immunoblotting demonstrated that SAHA activated tumor suppressors like p53 and Rb1 via phosphorylation and promoted cell apoptosis in NPC cells but inactivated energetic pathways such as AMPK signaling. Overall, our study indicated that SAHA exerted anti-tumor roles in NPC cells, which may serve as novel therapeutic for NPC patients.
A Prognostic Model for Colon Cancer Patients Based on Eight Signature Autophagy Genes
Authors: Xu Jiasheng , Dai Siqi , Yuan Ying , Xiao Qian , Ding Kefeng
Published: November 2020

Objective

To screen key autophagy genes in colon cancer and construct an autophagy gene model to predict the prognosis of patients with colon cancer.

Methods

The colon cancer data from the TCGA were downloaded as the training set, data chip of GSE17536 as the validation set. The differential genes of the training set were obtained and were analyzed for enrichment and protein network. Acquire autophagy genes from Human Autophagy Database www.autophagy.lu/project.html. Autophagy genes in differentially expressed genes were extracted using R-packages limma. Using LASSO/Cox regression analysis combined with clinical information to construct the autophagy gene risk scoring model and divide the samples into high and low risk groups according to the risk value. The Nomogram assessment model was used to predict patient outcomes. CIBERSORT was used to calculate the infiltration of immune cells in the samples and study the relationship between high and low risk groups and immune checkpoints.

Results

Nine hundred seventy-six differentially expressed genes were screened from training set, including five hundred sixty-eight up-regulated genes and four hundred eight down regulated genes. These differentially expressed genes were mainly involved: the regulation of membrane potential, neuroactive ligand-receptor interaction. We identified eight autophagy genes CTSD, ULK3, CDKN2A, NRG1, ATG4B, ULK1, DAPK1, and SERPINA1 as key prognostic genes and constructed the model after extracting the differential autophagy genes in the training set. Survival analysis showed significant differences in sample survival time after grouping according to the model. Nomogram assessment showed that the model had high reliability for predicting the survival of patients with colon cancer in the 1, 3, 5 years. In the high-risk group, the infiltration degrees of nine types of immune cells are different and the samples can be well distinguished according to these nine types of immune cells. Immunological checkpoint correlation results showed that the expression levels of CTLA4, IDO1, LAG3, PDL1, and TIGIT increased in high-risk groups.

Conclusion

The prognosis prediction model based on autophagy gene has a good evaluation effect on the prognosis of colon cancer patients. Eight key autophagy genes can be used as prognostic markers for colon cancer.
Bone Angiogenesis and Vascular Niche Remodeling in Stress, Aging, and Diseases
Authors: Stucker Sina , Chen Junyu , Watt Fiona E. , Kusumbe Anjali P.
Published: November 2020

The bone marrow (BM) vascular niche microenvironments harbor stem and progenitor cells of various lineages. Bone angiogenesis is distinct and involves tissue-specific signals. The nurturing vascular niches in the BM are complex and heterogenous consisting of distinct vascular and perivascular cell types that provide crucial signals for the maintenance of stem and progenitor cells. Growing evidence suggests that the BM niche is highly sensitive to stress. Aging, inflammation and other stress factors induce changes in BM niche cells and their crosstalk with tissue cells leading to perturbed hematopoiesis, bone angiogenesis and bone formation. Defining vascular niche remodeling under stress conditions will improve our understanding of the BM vascular niche and its role in homeostasis and disease. Therefore, this review provides an overview of the current understanding of the BM vascular niches for hematopoietic stem cells and their malfunction during aging, bone loss diseases, arthritis and metastasis.
HDAC6 Regulates the Fusion of Autophagosome and Lysosome to Involve in Odontoblast Differentiation
Authors: Zhan Yunyan , Wang Haisheng , Zhang Lu , Pei Fei , Chen Zhi
Published: November 2020

Odontoblast differentiation is an important process during tooth development in which pre-odontoblasts undergo elongation, polarization, and finally become mature secretory odontoblasts. Many factors have been found to regulate the process, and our previous studies demonstrated that autophagy plays an important role in tooth development and promotes odontoblastic differentiation in an inflammatory environment. However, it remains unclear how autophagy is modulated during odontoblast differentiation. In this study, we found that HDAC6 was involved in odontoblast differentiation. The odontoblastic differentiation capacity of human dental papilla cells was impaired upon HDAC6 inhibition. Moreover, we found that HDAC6 and autophagy exhibited similar expression patterns during odontoblast differentiation both in vivo and in vitro; the expression of HDAC6 and the autophagy related proteins ATG5 and LC3 increased as differentiation progressed. Upon knockdown of HDAC6, LC3 puncta were increased in cytoplasm and the autophagy substrate P62 was also increased, suggesting that autophagic flux was affected in human dental papilla cells. Next, we determined the mechanism during odontoblastic differentiation and found that the HDAC6 substrate acetylated-Tubulin was up-regulated when HDAC6 was knocked down, and LAMP2, LC3, and P62 protein levels were increased; however, the levels of ATG5 and Beclin1 showed no obvious change. Autophagosomes accumulated while the number of autolysosomes was decreased as determined by mRFP-GFP-LC3 plasmid labeling. This suggested that the fusion between autophagosomes and lysosomes was blocked, thus affecting the autophagic process during odontoblast differentiation. In conclusion, HDAC6 regulates the fusion of autophagosomes and lysosomes during odontoblast differentiation. When HDAC6 is inhibited, autophagosomes can't fuse with lysosomes, autophagy activity is decreased, and it leads to down-regulation of odontoblastic differentiation capacity. This provides a new perspective on the role of autophagy in odontoblast differentiation.
Carotid Intima-Media Thickness in Healthy Children and Adolescents: Normative Data and Systematic Literature Review
Authors: Drole Torkar Ana , Plesnik Emil , Groselj Urh , Battelino Tadej , Kotnik Primoz
Published: November 2020

Objectives: Early identification of children at risk of atherosclerosis is of paramount importance for implementing primary preventive measures addressing vascular health. Carotid intima-media thickness (cIMT) is a non-invasive biomarker of atherosclerosis. Semiautomatic radiofrequency-based software-guided technique quality intima-media thickness (RF-QIMT) was used to determine cIMT normative values in a healthy cohort of Caucasian children aged 6 to 18 years.

Study design: In a cross-sectional study, data on age, chronic illness, medication use, and pubertal status was acquired by a questioner. Anthropometric and blood pressure measurements were performed by standardized methods and trained medical personnel. cIMT of the right common carotid artery far wall (1 centimeter proximal to bifurcation) was determined using a multifrequency (3–13 MHz) electronic linear array transducer SL1543, a portable ultrasound device (MyLab Gamma Esaote, Genoa, Italy), and RF-QIMT software. A systematic review of the published normal cIMT in children was done using PRISMA methodology, and identified normative values were compared to those obtained in the presented study.

Results: 1137 non-obese normotensive children (males: n = 512; mean age 12.04 ± 3.52 years, females: n = 625, mean age 12.98 ± 3.83 years) were included. Gender-, age-, and height-specific mean cIMT percentile tables, percentile charts, and LMS tables for the RF-QIMT method were provided. They were comparable to the previously published data on mean cIMT gained by other validated ultrasound imaging techniques. cIMT increased with age, height, hip circumference, and BMI and was higher in males.

Conclusions: Gender-, age-, and height-specific normative cIMT values, using the semiautomatic software-guided RF-QIMT technique, in children aged 6 to 18 years were developed and validated in respect to the previously published pediatric normative cIMT data. It is suggested that the investigated method could be used for the estimation of atherosclerotic risk in children, especially in epidemiological studies.
Long-Term Outcomes of a Phase I Study With UV1, a Second Generation Telomerase Based Vaccine, in Patients With Advanced Non-Small Cell Lung Cancer
Authors: Brunsvig Paal F. , Guren Tormod Kyrre , Nyakas Marta , Steinfeldt-Reisse Claudius H. , Rasch Wenche , Kyte Jon Amund , Juul Hedvig Vidarsdotter , Aamdal Steinar , Gaudernack Gustav , Inderberg Else Marit
Published: November 2020

Human telomerase reverse transcriptase (hTERT) is a target antigen for cancer immunotherapy in patients with non-small cell lung cancer (NSCLC). We have tested a novel hTERT vaccine, UV1, designed to give high population coverage. UV1 is composed of three synthetic long peptides containing multiple epitopes identified by epitope spreading data from long-term survivors from previous hTERT vaccination trials. Eighteen non-HLA-typed patients with stage III/IV NSCLC with no evidence of progression after prior treatments, were enrolled in a phase I dose-escalation study of UV1 vaccination with GM-CSF as adjuvant, evaluating safety, immune response, and long-term clinical outcome. Treatment with UV1 was well tolerated with no serious adverse events observed. Seventeen patients were evaluable for tumor response; 15 patients had stable disease as best response. The median progression free survival (PFS) was 10.7 months, and the median overall survival (OS) was 28.2 months. The OS at 4 years was 39% (7/18). Five patients are alive (median survival 5.6 years), and none of these are known to have received checkpoint therapy after vaccination. UV1 induced specific T-cell responses in the majority (67%) of patients. Immune responses were dynamic and long lasting. Both immune response (IR) and OS were dose related. More patients in the highest UV1 dosage group (700 μg) developed IRs compared to the other groups, and the IRs were stronger and occurred earlier. Patients in this group had a 4-year OS of 83%. The safety and clinical outcome data favor 700 μg as the preferred UV1 dose in this patient population. These results provide a rationale for further clinical studies in NSCLC with UV1 vaccination in combination with immune checkpoint blockade.

Clinical Trial Registration

https://www.clinicaltrials.gov, identifier NCT0178909.