bluuebunny/crossref_metadata_2025 huggingface dataset:
Bicycle Injury Prevention Education Using 360° Virtual Reality Experiences of Accidents and Computer-Based Activity
Authors: Lee Woosup , Park Ju Ok , Hong Sung Ok , Kim Youngtaek
Published: October 2022

Injury prevention programs for children and adolescents need to be immersive and interactive. This study assessed a bicycle injury prevention program using technology-based education based on the Activated Health Education model and evaluated its effect on environmental factor awareness and attitude toward helmet-wearing. Using virtual reality technology, elementary and middle school students could experience simulated bicycle accidents. It was followed by an awareness phase that included a 30-min lecture where students self-learned and discussed risk-preventive factors. Students then developed user-created content and customized helmets they were given. We assessed students before the program, immediately afterward, and one month after the program. The number of respondents who said they were aware of surrounding bicycle lanes increased from 75.3% (pre-program) to 92.5% (one month after). Those who said they wore helmets often or always rose from 14.3% (pre-program) to 32.5% (one month later). The number needed to treat helmet-wearing behavior was approximately four, meaning that four people were required to participate in the program to have an impact on one person’s helmet-wearing. We found that virtual reality and computer-based activities can help children and adolescents experience bicycle accidents, be aware of risk factors, and change their behaviors responsibly.
Effect of Dornase Alfa on the Lung Clearance Index in Children with Cystic Fibrosis: A Lesson from a Case Series
Authors: Terlizzi Vito , Parisi Giuseppe Fabio , Ferrari Beatrice , Castellani Chiara , Manti Sara , Leonardi Salvatore , Taccetti Giovanni
Published: October 2022

Background: Dornase alfa (DNase) is the only mucus-degrading agent that has proven efficacy in cystic fibrosis (CF). Few studies have evaluated the effects of DNase on the lung clearance index (LCI). We report the experience of two CF centers in which LCI monitoring was used to evaluate the efficacy of DNase therapy. Methods: This is a prospective and observational study, evaluating the effects of DNase therapy on LCI values in three CF children followed at CF centers in Florence and Catania, Italy. In both centers, LCI was performed routinely, every 3–6 months, based on the clinical picture and severity of the lung disease. In this study, we evaluated the LCI before and after long-term DNase therapy. Results: DNase improved LCI values in the absence of respiratory exacerbations: in case n. 1 LCI decreased by 39% in 16 months (from 11.1 to 6.8); in case n. 2 by 20% in 12 months (from 9.3 to 7.4); in case n. 3 by 24% in 16 months (from 9.3 to 7.0). Conclusions: This case series confirms the efficacy of DNase therapy in CF children, as demonstrated by the LCI reduction in treated patients. Furthermore, our results suggest that LCI is a sensitive marker of disease and can be used for the evaluation of response to treatment.
Aromatic Polyphenol π-π Interactions with Superoxide Radicals Contribute to Radical Scavenging and Can Make Polyphenols Mimic Superoxide Dismutase Activity
Authors: Caruso Francesco , Incerpi Sandra , Pedersen Jens , Belli Stuart , Kaur Sarjit , Rossi Miriam
Published: October 2022

Polyphenols are valuable natural antioxidants present in our diet that likely mitigate aging effects, neurodegenerative conditions, and other diseases. However, because of their poor absorption in the gut and consequent low concentration in biological fluids (µM range), reservations about polyphenol antioxidant efficiency have been raised. In this review, it is shown that after scavenging superoxide radicals, coumarin, chalcone, and flavonoid polyphenols can reform themselves, becoming ready for additional cycles of scavenging, similar to the catalytic cycle in superoxide dismutase (SOD) action. The π-π interaction between one polyphenol ring and superoxide is associated with oxidation of the latter due to transfer of its unpaired electron to a polyphenolic aromatic ring, and consequent formation of a molecule of O2 (one product of SOD action). Mechanistically, it is very difficult to establish if this π-π interaction proceeds before or after the most common mode of scavenging superoxide, e.g., abstraction of an aromatic polyphenol H(hydroxyl), which then is used to form H2O2 (the other molecule produced by SOD action). At the end of this cycle of superoxide scavenging, 4-methyl-7,8-di-hydroxy-coumarin and the flavonoid galangin reform themselves. An alternative mechanistic pathway by galangin forms the η-(H2O2)-galangin-η-O2 complex that includes additional H2O2 and O2 molecules. Another mode of action is seen with the chalcone butein, in which the polyphenol system incorporates a molecule of O2, e.g., a η-O2-butein complex is formed, ready for additional scavenging. Of the several families of polyphenols analyzed in this review, only butein was able to circumvent an initial π-π interaction, directing the superoxide towards H(hydroxyl) in position 4, e.g., acting as a typical polyphenol scavenger of superoxide. This fact did not impede an additional superoxide to later react with the aromatic ring in π-π fashion. It is concluded that by mimicking SOD enzyme action, the low concentration of polyphenols in biological fluids is not a limiting factor for effective scavenging of superoxide.
Bonding Mechanism and Process Characteristics of Special Polymers Applied in Pelletizing Binders
Authors: Zhao Hongxing , Zhou Fengshan , Ma Cunfa , Wei Zhongjin , Long Wenjun
Published: October 2022

Pellet ore not only has excellent metallurgical and mechanical properties, but is also an important metallurgical raw material used to solve the problem of increasing depletion of global high-grade iron ore resources. Bentonite has long been widely used in pellet ore production, which is not only expensive but also causes serious metallurgical pollution. Organic binders can form stronger adhesion and cohesion with mineral particles inside the green pellets than capillary forces, which greatly improves the pelletizing rate and significantly increases the strength of green and dry pellets, and it becomes an indispensable alternative to bentonite because it volatilizes pyrolytically at high temperatures, leaving almost no inorganic contaminants inside the pellet ore. In order to let more pellet researchers fully understand the research status and pelletizing theory of organic binders, this review systematically summarizes seven common organic binders, and elaborates on their adhesion mechanism and process characteristics, so as to provide references for pellet researchers and readers to further prepare cost-effective pellet binders and improve advanced pelletizing technology.
Identification of the Angle Errors of the LED Parallel-Light Module in PCB Exposure Device by Using Neural Network Learning Algorithms
Authors: Chen Chi-Feng , Chen Jian-Rong , Chen Ting-Yu
Published: October 2022

For the smart manufacturing development of printed-circuit-board (PCB) exposure devices, the LED parallel-light (LPL) module is investigated and the angle errors of those LPL units are identified by neural network learning algorithms. At present, in PCB manufacturing, most circuit boards use photoresist covering etching. After exposure and development, unwanted copper foil is etched and removed to make circuit boards. The exposure process is its key process, and the equipment used in this process is an exposure machine. The LPL unit is designed and the LPL exposure module is searched under the principle of higher irradiance uniformity. The learning data of supervised learning for the convolutional neural network (CNN) include a 2D irradiance distribution image constructed by the ray tracing simulation tool. In these supervised learning data, all units of LPL-EM are randomly added with a self-specific angle error. By using Fast Region-based CNN, the identification of the multi-LPL module with the specific errors of inclination and azimuth angle is verified. Those results preliminarily illustrate that supervised learning techniques should be able to help identify the errors of inclination and azimuth angle for the single LPL unit and multi-light module of PCB exposure devices. In other words, this technology should serve as a reference for the development of the PCB exposure process towards smart manufacturing.
Structure-Property Correlation in Sodium Borophosphate Glasses Modified with Niobium Oxide
Authors: Mošner Petr , Hostinský Tomáš , Koudelka Ladislav , Razum Marta , Pavić Luka , Montagne Lionel , Revel Bertrand
Published: October 2022

Bulk glasses of the series (100−x)[0.4Na2O-0.2Nb2O5-0.4P2O5]-xB2O3 with x = 0–48 mol% B2O3 were prepared by slow cooling in air. Their glass transition temperature increases within the range of 0–16 mol% B2O3, but further additions of B2O3 result in its decrease. Their structure was investigated by Raman, 11B, and 31P MAS NMR spectroscopy. The relative number of BO4 units decreases with increasing B2O3 content, while the number of BO3 units increases up to 59 % at x = 48. The upfield shift of a broad resonance peak in the 31P MAS NMR spectra is ascribed to an increasing connectedness of the structural network with increasing B2O3 content. Strong Raman band at 916–929 cm−1 shows on the presence of NbO6 octahedra in the structural network of these glasses. With the B2O3 addition, a decrease in DC conductivity is observed, which is attributed to the decrease in the concentration of Na+ ions.
Short-Lived Aggregations of Filograna/Salmacina Tube Worms in the Gulf of Oman
Authors: Samimi-Namin Kaveh , ten Hove Harry A. , Claereboudt Michel R. , Paulay Gustav , Hoeksema Bert W.
Published: October 2022

Dense aggregations of serpulid worms were encountered in the Daymaniyat Islands (Gulf of Oman) from 10 to 20 m depth, over the period January–March, 2021. The species responsible for these aggregations belongs to the Filograna/Salmacina-complex (Annelida: Serpulidae). This species has been present in the area and observed along the Oman coastline, but high-density aggregates like this have not been reported before. The most probable cause of the aggregations, supported by field observations and Aqua-MODIS satellite data, was natural eutrophication with a subsequent algal bloom linked to the local winter monsoon. This observation emphasises the importance of documenting biodiversity and dynamics of reef communities along the Oman coastline.
Understanding the Taxonomic Complexes and Species Delimitation within Sambucus L. (Viburnaceae)
Authors: Waswa Emmanuel Nyongesa , Mutinda Elizabeth Syowai , Mkala Elijah Mbandi , Katumo Daniel Mutavi , Oulo Millicent Akinyi , Odago Wyclif Ochieng , Amenu Sara Getachew , Ding Shi-Xiong , Hu Guang-Wan
Published: October 2022

The genus Sambucus belongs to the family Viburnaceae, and has long been a debatable taxon regarding its systematics and taxonomic assignment. It is known for its morphological and genetic variations and thus the taxonomic delimitation remains uncertain. Phenotypic and genotypic variations within plant species causes difficulties in the detection of species boundaries. In the present review, we compiled a set of studies that assessed species discrimination within Sambucus genus by morphological and molecular markers as well as the taxonomic uncertainties in the re-circumscription of the segregated genera i.e., Viburnum L., Sambucus L., and Adoxa L., (syn. Sinadoxa C.Y. Wu, Z.L. Wu & R.F. Huang/Tetradoxa C.Y. Wu) from Caprifoliaceae and their family assignment. The data was obtained from published journal articles and various online databases. Morphological and molecular diagnostic characters were employed to distinguish Sambucus species. Our findings indicated that high intraspecific variations within narrow-ranged populations and their widespread counterparts contribute to its taxonomic complications. Additionally, intraspecific variations within Sambucus species leads to misidentifications and the misapplication of names, occasioning the publication of many putative synonyms and ambiguous names. Possible interspecific hybridizations experienced between Sambucus species increases complexity in character evolution. A morphological analysis showed that the endocarp seed morphological characters can reliably support the segregation of Sambucus taxa into discrete taxonomic entities. In molecular analysis, the NeighborNet (NN) phylogenetic split network revealed three distinct genera including Viburnum, Sambucus, and Adoxa, which clustered separately. The Maximum Likelihood (ML) analysis indicated that the Sambucus species formed a monophyletic group and clustered into two major clades, a small clade containing S. maderensis, S. peruviana, S. nigra, and S. canadensis, and a large clade encompassing the rest of the species with significantly supported clades (<52%). The preferred family assignment for Sambucus is Viburnaceae.
New Records of the Cryptogenic Soft Coral Genus Stragulum (Tubiporidae) from the Eastern Caribbean and the Persian Gulf
Authors: Samimi-Namin Kaveh , van Ofwegen Leen P. , Hoeksema Bert W. , Woodall Lucy C. , Meijer zu Schlochtern Melanie , McFadden Catherine S.
Published: October 2022

The monotypic soft coral genus Stragulum van Ofwegen and Haddad, 2011 (Octocorallia: Malacalcyonacea: Tubiporidae) was originally described from Brazil, southwest Atlantic Ocean. Here, we report the first records of the genus from the eastern Caribbean and the Persian Gulf in the northwest Indian Ocean. We compare the morphological features of specimens, together with molecular data from three commonly used barcoding markers (COI, mtMutS, 28S rDNA) and 308 ultraconserved elements (UCE) and exon loci sequenced using a target-enrichment approach. The molecular and morphological data together suggest that specimens from all three localities are the same species, i.e., Stragulum bicolor van Ofwegen and Haddad, 2011. It is still not possible to establish the native range of the species or determine whether it may be an introduced species due to the limited number of specimens included in this study. However, the lack of historical records, its fouling abilities on artificial substrates, and a growing number of observations support the invasive nature of the species in Brazilian and Caribbean waters and therefore suggest that it may have been introduced into the Atlantic from elsewhere. Interestingly, the species has not shown any invasive behaviour in the Persian Gulf, where it has been found only on natural, rocky substrates. The aim of the present report is to create awareness of this taxon with the hope that this will lead to new records from other localities and help to establish its native range.
Using Import Data to Predict the Potential of Introduction of Alert Alien Species to South Korea
Authors: Jo Aram , Son Seunghun , Kim Dongeon
Published: October 2022

As globalization progresses, human activities, such as travel and trade, are rapidly increasing beyond national boundaries. It is increasingly recognized that places, such as ports and airports, where trade occurs play a major role as an introduction pathway for alien species. In this study, we focused on evaluating the possibility of introduction of Alert Alien Species (AAS) through trade data among countries. The natural and distribution range of AAS were analyzed along with import data by country. There were large differences between the number of AAS distributed in a country and the import weight of items related to the import of AAS from the country. Fish, which account for 76% of the import weight of AAS, 43 and 40 species of the 84 species of AAS were distributed in US and Russia, respectively. However, the import weight of items related to the import of fish designated as AAS from these countries were extremely low. This finding suggests that trade, which is the main introduction pathway, is not taken into account in the designation of AAS. For future management plans for non-introduced alien species, species with a high possibility of introduction into South Korea through trade should be prioritized using import data.
The Plight of Female Entrepreneurs in India
Authors: Aggrawal Artee , Carrick Jon , Kennedy Jeffrey , Fernandez Giovanni
Published: October 2022

Women from around the world are making substantive contributions to new ventures. Research on this phenomenon is starting to increase, but to this point, most of the research on female entrepreneurship has only examined it from the perspective of developed countries. As such, there is a gap in understanding this phenomenon from the perspective of developing countries. Due to the lack of theoretical underpinnings, and exploratory research approach to unearthing new ideas was called for. Using 10 female entrepreneurs as case studies and depth interviews as the main source of data, this study explores female entrepreneurship in India. The study’s findings suggest that deep-rooted gender bias and family pressures are major challenges that female entrepreneurs in India face. This study’s findings also suggest that supportive husbands, stress management, and maintaining a good work–life balance are important to helping female high-tech entrepreneurs in India overcome the challenges that they face. The results from this study inform the literature on the plight of female entrepreneurs in India, and more broadly, the study contributes to the understanding of the challenges that female entrepreneurs all around the world face.
FIRN: A Novel Fish Individual Recognition Method with Accurate Detection and Attention Mechanism
Authors: Gao Chunqi , Wu Junfeng , Yu Hong , Yin Jianhao , Guo Shihao
Published: October 2022

Fish individual recognition technology is one of the key technologies to realize automated farming. Aiming at the deficiencies in the existing animal individual recognition technology, this paper proposes a method for individual recognition of underwater fish based on deep learning technology, which is divided into two parts: fish individual object detection and fish individual recognition. In the object detection part, the research has improved a new object detection for underwater fish based on the YOLOv4 algorithm, which changed the feature extraction network in YOLOv4 from CSP Darknet53 to Mobilenetv3 and changed the 3 × 3 convolution in the enhanced feature extraction network PANet to depthwise separable convolution. Compared with the original YOLOv4, the mean average precision is improved by 1.97%. For individual recognition, an algorithm called FIRN (Fish Individual Recognition Network) for individual recognition of underwater fish is proposed. The feature extraction network of the algorithm uses the improved ResNext50, and the loss function uses Arcface Loss. The CBAM attention module is introduced in the residual block of ResNext50, the max-pooling layer in the trunk is removed, and dilated convolution is introduced in the residual block, which increases the receptive field and improves the ability of feature extraction. Experiments show that the FIEN algorithm can enhance the compactness within a class while ensuring the separability between classes, and has a better recognition effect than other algorithms.
Determination of Excitation Amplitude and Phase for Wide-Band Phased Array Antenna Based on Spherical Wave Expansion and Mode Filtering
Authors: Su Yao , Song Zixuan , Zhang Shuai , Gong Shuxi
Published: October 2022

A new method for solving the excitation amplitude and phase of wide-band phased array antenna is presented, in which spherical wave expansion and mode filtering (SWEMF) techniques are applied for the first time. Different from the previous methods that are required of matrix inversion or optimization iteration, the proposed SWEMF method is a forward calculation process. Thus, the solution is unique, and the result is closer to the true value. On the other hand, the SWEMF method only needs the total radiated field data of the array antenna in a small angular domain to ensure that the operation is simple and efficient. The effectiveness of the SWEMF method is successfully verified by examples of low sidelobe planar and linear arrays. The mean square error of the excitation amplitude can reach −38.88 dB. The range of excitation amplitude error is 0.05 v, and the excitation phase error is within 5.2°. This method takes about 60 s to calculate amplitude and phase at any one time. The feed amplitude and phase can be only calculated with the data in a small angular domain, and when the amount of data is small.
Electric Power Quality through Analysis and Experiment
Authors: Popa Gabriel
Published: October 2022

The quality of electrical energy is of particular importance for power engineering. This study presents an analysis of articles made in the Special Issue “Analysis and Experiments for Electric Power Quality”. As techniques and technology advance, electrical consumers and equipment become more sensitive to disturbances in the electrical network (in particular, low- and medium-voltage). It can lead to costly outages and lost production, which affect productivity. The analyzed articles present interesting technical studies made on industrial and nonindustrial consumers, of low- and medium-voltage, from the point of view of the quality of electricity. Voltage and current harmonics, voltage sags and swells, interruptions, unbalance, and low power factor will lead to higher electricity bills, overloading, and rapid aging of electrical networks and electric equipment. The power quality depends not only on the supplier but also on all consumers connected to the same power network; some can cause disruptive influences in the supply network, affecting the operation of other consumers. Ensuring the power quality of industrial and nonindustrial applications is an objective difficult to achieve.
Wettability after Artificial and Natural Weathering of Polyethylene Terephthalate
Authors: Benke Annegret , Sonnenberg Johanna , Oelschlägel Kathrin , Schneider Markus , Lux Milena , Potthoff Annegret
Published: October 2022

The weathering of plastics is always accompanied by a change in surface properties, especially wettability in the case of water. For plastics weathering in an aquatic environment, wettability plays an important role in transport, sedimentation, and dispersion in the water body. To quantify wettability, contact angle measurement is a fast and convenient method that requires little experimental effort. This technique was used with the aim of systematically discussing how measured values of contact angles can contribute to the assessment of the weathering state. Using polyethylene terephthalate (PET), wetting was analyzed on samples from artificial weathering and from controlled, natural weathering. Surface analytical methods were used (Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), ultraviolet and visible light spectroscopy (UV/VIS)) to analyze the parameters affecting the contact angle: (i) chemical bond breaking and formation, (ii) eco-corona formation and biofilm growth, and (iii) change in surface structure and roughness. It was found that wettability with water increased during weathering in all cases. The reasons for this varied and depended on the method of weathering. The improved wettability during artificial weathering was due to changes in the polymer surface chemistry. In natural weathering, however, the formation of eco-corona and biofilm was responsible for the changes.
Tenacibaculum ovolyticum 16S rDNA Quantitative-PCR Assay Development and Field Testing
Authors: Nowlan Joseph P. , Heese Brianna M. , Wilson Matthew J. , Britney Scott R. , Lumsden John S. , Russell Spencer
Published: October 2022

In British Columbia (BC; Canada) Atlantic salmon (Salmo salar L.) production, Tenacibaculum members are associated with ‘mouthrot’ and disease identification is based on gross observation and clinical data. Genomic similarities (i.e., putative virulence factors) between T. ovolyticum and other better-characterized agents of mouthrot could imply potential pathogenicity. While T. ovolyticum has not been directly linked to salmon mortality events in BC, it has been isolated from diseased marine fish. To investigate T. ovolyticum’s pathogenicity in situ, a T. ovolyticum 16S rDNA qPCR assay targeting a ~155 bp amplicon was developed. The assay was used to screen 67 biotic and 33 abiotic samples collected from a BC Atlantic salmon (Salmo salar L.) net-pen site before, during, and after a mouthrot outbreak. The assay was specific, quantifiable and detectable for T. ovolyticum over 6-log and 8-log units, respectively. However, cycle quotients differed between the BC isolate and type strain of T. ovolyticum, suggesting that qualitative use of the qPCR assay in field samples would be more accurate. Only two out of 100 samples were T. ovolyticum-positive, indicating limited involvement in this particular outbreak. However, the ecological role of T. ovolyticum and its involvement in the pathogenesis of other mouthrot outbreaks in Atlantic salmon is unknown.
New Report of Zu cristatus (Bonelli, 1819) in the Ionian Sea with an In-Depth Morphometrical Comparison with All Mediterranean Records
Authors: Albano Marco , D’Iglio Claudio , Spanò Nunziacarla , Di Paola Davide , Alesci Alessio , Savoca Serena , Capillo Gioele
Published: October 2022

Monitoring the occurrence and distribution of rare fish species is essential, especially in a semi-closed basin such as the Mediterranean Sea. Biologically interesting species often represent fishing waste (being part of the by-catch) and therefore are not retained, leading to a huge gap in data collection. Here we report about the occurrence in the Ionian Sea of a Zu cristatus (Bonelli, 1819) specimen, a mesopelagic fish from the order Lampriformes considered rare in the whole Mediterranean basin. The sample was captured at a depth of 720 m off the coast of Noto (Sicily, Italy) by deep-sea longline fisheries. The biometrics and meristic data were compared with all the available data on specimens previously reported in the Mediterranean Sea. It is interesting to note that, among the few records in the literature, most reports mention a lower depth range for this species. In fact, it is quite known that juvenile specimens of Z. cristatus were frequently found in shallow waters, while information about the biology of the adult stage is scarce, indicating they would seem to prefer much deeper environments. Considering the difficulty to collect data on this rare fish, this contribution can help to improve the knowledge about its distribution.
Application of Edible Coating Based on Liquid Acid Whey Protein Concentrate with Indigenous Lactobacillus helveticus for Acid-Curd Cheese Quality Improvement
Authors: Vasiliauskaite Agne , Mileriene Justina , Songisepp Epp , Rud Ida , Muizniece-Brasava Sandra , Ciprovica Inga , Axelsson Lars , Lutter Liis , Aleksandrovas Elvidas , Tammsaar Ene , Salomskiene Joana , Serniene Loreta , Malakauskas Mindaugas
Published: October 2022

Edible coatings as carriers for protective lactic acid bacteria (LAB) can enhance hygienic quality to dairy products. Thus, the aim of this study was to improve the quality of artisanal acid-curd cheese by applying liquid acid whey protein concentrate based edible coating with entrapped indigenous antimicrobial Lactobacillus helveticus MI-LH13. The edible fresh acid-curd cheese coating was composed of 100% (w/w) liquid acid whey protein concentrate (LAWPC), apple pectin, sunflower oil, and glycerol containing 6 log10 CFU/mL of strain biomass applied on cheese by dipping. The cheese samples were examined over 21 days of storage for changes of microbiological criteria (LAB, yeast and mould, coliform, enterobacteria, and lipolytic microorganism), physicochemical (pH, lactic acid, protein, fat, moisture content, and colour), rheological, and sensory properties. The coating significantly improved appearance and slowed down discolouration of cheese by preserving moisture during prolonged storage. The immobilisation of L. helveticus cells into the coating had no negative effect on their viability throughout 14 days of storage at 4 °C and 23 °C. The application of coating with immobilised cells on cheeses significantly decreased the counts of yeast up to 1 log10 CFU/g during 14 days (p < 0.05) of storage and suppressed growth of mould for 21 days resulting in improved flavour of curd cheese at the end of storage. These findings indicate that LAWPC-pectin formulation provided an excellent matrix to support L. helveticus cell viability. Acting as protective antimicrobial barrier in fresh cheeses, this bioactive coating can reduce microbial contamination after processing enabling the producers to extend the shelf life of this perishable product.
Characterization of Flavor Compounds in Distilled Spirits: Developing a Versatile Analytical Method Suitable for Micro-Distilleries
Authors: Barnes Quentin , Vial Jérôme , Thiébaut Didier , De Saint Jores Clément , Steyer Damien , Contamin Marie-Anne , Papaiconomou Nicolas , Fernandez Xavier
Published: October 2022

Over the last few years, the development of micro-distilleries producing diverse spirits with various flavors has been observed. Versatile analytical techniques for the characterization of aroma compounds in such alcoholic beverages are therefore required. A model mixture embodying a theoretical distilled spirit was made according to the data found in literature to compare usual extraction techniques. When it was applied to the model liquor, the headspace solid phase microextraction (HS-SPME) extraction method was preferred to the liquid-liquid extraction (LLE), solid phase extraction (SPE) and stir bar/headspace sorptive extraction (SBSE/HSSE) methods according to efficiency, cost, and environmental criteria. An optimization study using the model mixture showed that the extraction was optimal with a divinylbenzene/carboxen/poly(dimethylsiloxane) DVB/CAR/PDMS fiber, during 60 min, at 35 °C and with the addition of 10% NaCl. This method was successfully applied to three different commercial liquors and led to the identification of 188 flavor compounds, including alcohols, esters, lactones, carbonyls, acetals, fatty acids, phenols, furans, aromatics, terpenoids, alkenes, and alkanes.
The Influence of Prebiotics on Wheat Flour, Dough, and Bread Properties; Resistant Starch, Polydextrose, and Inulin
Authors: Ansari Fereshteh , Pimentel Tatiana Colombo , Pourjafar Hadi , Ibrahim Salam A. , Jafari Seid Mahdi
Published: October 2022

The addition of prebiotics to bread is one of the most important ways to improve its techno-functional properties. In this study, the effects of resistant starch, polydextrose, and inulin on wheat flour, dough, and bread properties were investigated. The farinography results showed that resistant starch significantly increased the development time (2:18) via a boosting effect; however, polydextrose (1:48) and inulin (1:36) weakened the dough (p < 0.05). Inulin, polydextrose, and resistant starch had the greatest effect on reducing water absorption (40, 43.2, and 48.9), respectively, (p < 0.05). According to extensography data, the addition of inulin produced the best result in baking compared to other polysaccharides. In terms of baked breads, the samples containing resistant starch had high moisture content that could be due to starch gelatinization and moisture-retention, which delays the staling process of the bread. Inulin, polydextrose, and resistant starch prebiotic ingredients affected the rheological properties of the dough, overall bread quality and organoleptic characteristics; however, resistant starch was the best prebiotic used in this study.
Principle and Application of Steam Explosion Technology in Modification of Food Fiber
Authors: Ma Chao , Ni Liying , Guo Zebin , Zeng Hongliang , Wu Maoyu , Zhang Ming , Zheng Baodong
Published: October 2022

Steam explosion is a widely used hydrothermal pretreatment method, also known as autohydrolysis, which has become a popular pretreatment method due to its lower energy consumption and lower chemical usage. In this review, we summarized the technical principle of steam explosion, and its definition, modification and application in dietary fiber, which have been explored by researchers in recent years. The principle and application of steam explosion technology in the modification of food dietary fiber were analyzed. The change in dietary fiber structure; physical, chemical, and functional characteristics; the advantages and disadvantages of the method; and future development trends were discussed, with the aim to strengthen the economic value and utilization of plants with high dietary fiber content and their byproducts.
Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea
Authors: Luo Yan , Zhao Zhenjun , Chen Hujiang , Pan Xueli , Li Risheng , Wu Dewen , Hu Xianchun , Zhang Lingling , Wu Huawei , Li Xinghui
Published: October 2022

Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples. FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed. The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides. The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation. According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1. The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides. Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages. The study will further help to reveal the function of tea polysaccharides and promote their practical application as a functional food.
The Protective Effects of Iron Free Lactoferrin on Lipopolysaccharide-Induced Intestinal Inflammatory Injury via Modulating the NF-κB/PPAR Signaling Pathway
Authors: Wu Hongya , Fan Linlin , Gao Yanan , Wang Jiaqi , Zheng Nan
Published: October 2022

Research evidence shows that effective nutritional intervention could prevent or reduce intestinal inflammatory injury in newborn infants. Iron free lactoferrin (apo-LF), one of the main types of lactoferrin (LF), is a bioactive protein in milk that plays a vital role in maintaining intestinal health. The potential mechanism by which apo-LF modulates intestinal inflammation is, however, still unclear. In the study we first explored key genes and pathways in vitro by transcriptome date analysis and then validated them in vivo to reveal the underlying molecular mechanism. The results showed that apo-LF pretreatment effectively inhibited lipopolysaccharide (LPS)-induced primary intestinal epithelial cells (IECs) inflammation in the co-culture system (primary IECs and immune cells), which was specifically manifested as the reduction of the concentration of TNF-α, IL-6 and IL-1β and increased the concentration of IFN-γ. In addition, transcriptome data analysis revealed that the key pathway for apo-LF to exert anti-inflammatory effects was the NF-κB/PPAR signaling pathway. Further validation was performed using western blotting in colonic tissues of young mice and it was found that the major proteins of NF-κB signaling pathway (NF-κB, TNF-α and IL-1β) were inhibited by apo-LF and the target proteins of PPAR signaling pathway (PPAR-γ and PFKFB3) were activated by apo-LF. Taken together, this suggests that apo-LF has a protective effect against LPS-induced intestinal inflammatory injury via modulating the NF-κB/PPAR signaling pathway, which provides new insights for further anti-inflammatory study of apo-LF.
Elemental Laser-Plasma Analysis of Pointed Gourd Leaves for Diabetes Management
Authors: Kumar Tejmani , Rai Prashant K. , Rai Abhishek K. , Rai Nilesh K. , Rai Awadhesh K. , Parigger Christian G. , Watal Geeta , Yadav Suman
Published: October 2022

This interdisciplinary work communicates the identification and quantification of elements responsible for the bioactive potency of leaves from pointed gourd, trichosanthes dioica, using laser-induced breakdown spectroscopy (LIBS). Calibration-free LIBS determines the presence of various trace and major elements, their concentrations, and ratios in which they are present in the leaves. The presence of specific elemental ratios of magnesium/sodium and magnesium/potassium could be promising for managing diabetes mellitus. Variable doses of aqueous extract from trichosanthes dioica leaves are administered for determination of the most effective one. Based on encouraging results, the extract could be harvested to serve as anti-diabetic medication for diabetes and associated symptoms.
Human Induced Pluripotent Stem Cell Phenotyping and Preclinical Modeling of Familial Parkinson’s Disease
Authors: Kim Jeffrey , Daadi Etienne W. , Oh Thomas , Daadi Elyas S. , Daadi Marcel M.
Published: October 2022

Parkinson’s disease (PD) is primarily idiopathic and a highly heterogenous neurodegenerative disease with patients experiencing a wide array of motor and non-motor symptoms. A major challenge for understanding susceptibility to PD is to determine the genetic and environmental factors that influence the mechanisms underlying the variations in disease-associated traits. The pathological hallmark of PD is the degeneration of dopaminergic neurons in the substantia nigra pars compacta region of the brain and post-mortem Lewy pathology, which leads to the loss of projecting axons innervating the striatum and to impaired motor and cognitive functions. While the cause of PD is still largely unknown, genome-wide association studies provide evidence that numerous polymorphic variants in various genes contribute to sporadic PD, and 10 to 15% of all cases are linked to some form of hereditary mutations, either autosomal dominant or recessive. Among the most common mutations observed in PD patients are in the genes LRRK2, SNCA, GBA1, PINK1, PRKN, and PARK7/DJ-1. In this review, we cover these PD-related mutations, the use of induced pluripotent stem cells as a disease in a dish model, and genetic animal models to better understand the diversity in the pathogenesis and long-term outcomes seen in PD patients.
Genetic Diversity and Population Genetic Structure of Aedes albopictus in the Yangtze River Basin, China
Authors: Zhang Heng-Duan , Gao Jian , Li Chun-Xiao , Ma Zu , Liu Yuan , Wang Ge , Liu Qing , Xing Dan , Guo Xiao-Xia , Zhao Teng , Jiang Yu-Ting , Dong Yan-De , Zhao Tong-Yan
Published: October 2022

Aedes albopictus is an indigenous primary vector of dengue and Zika viruses in China. Understanding the population spatial genetic structure, migration, and gene flow of vector species is critical to effectively preventing and controlling vector-borne diseases. The genetic variation and population structure of Ae. albopictus populations collected from 22 cities along the Yangtze River Basin were investigated with nine microsatellite loci and the mitochondrial CoxI gene. The polymorphic information content (PIC) values ranged from 0.534 to 0.871. The observed number of alleles (Na) values ranged from 5.455 to 11.455, and the effective number of alleles (Ne) values ranged from 3.106 to 4.041. The Shannon Index (I) ranged from 1.209 to 1.639. The observed heterozygosity (Ho) values ranged from 0.487 to 0.545. The FIS value ranged from 0.047 to 0.212. All Ae. albopictus populations were adequately allocated to three clades with significant genetic differences. Haplotype 2 is the most primitive molecular type and forms 26 other haplotypes after one or more site mutations. The rapid expansion of high-speed rail, aircraft routes and highways along the Yangtze River Basin have accelerated the dispersal and communication of mosquitoes, which appears to have contributed to inhibited population differentiation and promoted genetic diversity among Ae. albopictus populations.
Portable Digital Monitoring System for Sarcopenia Screening and Diagnosis
Authors: Teixeira Eduardo , Bohn Lucimére , Guimarães José Pedro , Marques-Aleixo Inês
Published: October 2022

Sarcopenia is a well-known highly prevalent muscle disease that severely impairs overall physical performance in elders, inducing a massive health-related economic burden. The widespread screening, diagnosis and treatment of sarcopenia are pivotal to restrain the disease progression and constrain its societal impact. Simple-to-use, portable, and reliable methods to evaluate sarcopenia are scarce, and sarcopenia-related assessments are typically done in several time-consuming stages. This study presents a portable digital system that enables a simple and intuitive method to evaluate sarcopenia—based on the European Working Group on Sarcopenia in Older People 2 (EWGSOP2) algorithm—including the four Find-Assess-Confirm-Severity (FACS) steps. The system comprises a mobile application (app); two wireless devices: a dynamometer (Gripwise) and a skinfold caliper (Lipowise); and a back-end website. To find cases, the SARC-F questionnaire is applied. To assess sarcopenia, the handgrip strength and the sit-to-stand tests are performed with the Gripwise and an application-embedded stopwatch, respectively. To confirm cases, anthropometric measures are performed, and muscle quantity is estimated with Lipowise. Finally, to assess severity, the app stopwatch grants the gait speed test application, evaluating physical performance. This step-by-step sarcopenia assessment results in a final grading according to the cut-off points of the EWGSOP2 criteria. All data is automatically encrypted and exported into a GDPR-compliant cloud platform, in which healthcare professionals can access and monitor their patients through the internet.
A Sign of Good Taste: Mori Ōgai, Mitsukoshi, and the Concept of Shumi
Authors: van der Meer Jurriaan
Published: October 2022

This paper attempts to situate the notion of shumi as a rhetorical device used by modern Japanese department stores as part of their marketing strategies. Although often equated with the concept of ‘taste’, I demonstrate how shumi both overlaps with and differs from the concept of taste, as it is often discussed in critical theory in the context of consumerism. I do this by examining how shumi was used in the PR-magazines of various department stores and other related forms of print media. Special attention is paid to the PR-magazine of Mitsukoshi, which is perhaps the most innovative department store in modern Japanese history. Subsequently, I analyze three short stories by Mori Ōgai (1862–1922) published in Mitsukoshi’s PR-magazine between 1911 and 1912. Mitsukoshi printed short stories by acclaimed authors in their magazines, mostly as a form of lighthearted entertainment and branding. Yet, when read closely, Ōgai’s three stories also form a profound observation of the skewed moral reality of a market-driven economy. Each of the narratives under scrutiny in this paper shows the human cost of a system in which social relations are dictated by consumer objects. The cultivation of the urge to consume was carefully framed around the rhetoric of shumi and was thus not merely a marketing tool to increase profit margins but also a mechanism to manipulate the desires and anxieties of consumers. A reading of Ōgai’s three short narratives reveals the ambivalent morality produced by the rhetoric of shumi, which in turn engendered and validated the identities of an emerging middle class through the consumable object-as-sign.
Exploring the Feasibility of an Online Diabetes Wellness Programme among Periodontitis Patients with Type II Diabetes Mellitus during the COVID-19 Pandemic
Authors: Rusly Nur Fadzlin Syahira , Jamil Nor Aini , Mohd-Dom Tuti Ningseh , Rani Haslina , Mohd-Said Shahida , Mohd-Norwir Nur Adila , Hamat Afendi
Published: October 2022

There were massive interruptions, including patient visits for dietary advice and dental services, during the COVID-19 outbreak. This study assessed the feasibility of an online diabetes wellness programme among periodontitis patients with type II diabetes mellitus. Patients were grouped into the intervention group (IG) and control group (CG). At baseline and week 12, all patients answered online questionnaires on diabetes-related knowledge, physical activity, and oral impacts on daily performances (OIDP). Body weight and waist circumference were self-measured with guided instructions. Diet recalls were used to estimate dietary and added sugar intake. All patients received a weekly educational video, but the IG attended three e-consultation sessions with a dietitian (weeks 1, 3, and 8) and a dentist (week 8) via video call. A semi-structured interview was conducted to collate qualitative feedback among the IG participants at the end of the programme. A total of 24 periodontitis patients (14 IG and 10 CG) participated in this study. Among the IG patients, significant improvements in diabetes knowledge, body weight, BMI, calories, carbohydrates, fat, and added sugar intakes were observed at week 12. The CG patients only had a significant reduction in carbohydrate intake post intervention. No changes were reported in physical activity level and OIDP for both groups. Feedback received from the IG participants included convenience, practical, preferred approach during the pandemic, increased awareness and knowledge, and favourable lifestyle changes. This study demonstrates that an online diabetes wellness programme with healthcare professionals is feasible and can facilitate knowledge and lifestyle improvements that can be adapted during the crisis situation.
The Process of Creating a New Brand Name for a Fruit Variety: A Review and Suggested Improvements
Authors: Arthur Jennifer , Bejaei Masoumeh
Published: October 2022

In an effort to protect intellectual property beyond patent and plant breeders’ rights and as a marketing tool to increase and maintain sales, the creation and trademarking of brand names for fruit is growing and gaining importance in the fruit industry. New fruit varietals, especially from long-lived tree fruits and vines, take many years of research to develop and bring to market. Differentiating what is essentially a commodity product is difficult, especially given bulk sales and packaging limitations. A distinctive brand name can be a powerful method of differentiating a new fruit from its competitors. To the best of our knowledge there has not been any study examining the process of brand name creation for fruits. This English language literature review examines the brand name creation process overall. A step-by-step process is discussed and situated in the context of fruits. Research on the overall process is dated: We propose a new preliminary research step to improve the process and discuss the need for future research on the role of the Internet and social media in the naming process. An overview of trademark considerations is provided. Knowledge of this process will assist breeders and marketers with brand name creation whether achieved internally or through an external agency or combination thereof.