bluuebunny/crossref_metadata_2025 huggingface dataset:
PONV management in patients with QTc prolongation on the EKG
Authors: Soghomonyan S. , Stoicea N. , Ackermann W. , Bhandary S. P.
Published: January 2021

Postoperative nausea and vomiting (PONV) is a commonly encountered problem in surgical practice. It delays discharge from the post-anesthesia care unit, requires additional resources to treat, and may increase the morbidity in some patients. Many effective drugs are available to treat or prevent PONV, however many of these drugs have the potential to prolong the QTc on the electrocardiogram (EKG) and increase the risk of serious ventricular arrhythmias, in particular, torsade de pointes. The QTc prolongation may be a manifestation of a genetic mutation resulting in abnormal myocyte repolarization or it may be acquired and associated with the use of various medications, electrolyte disorders, and physiological conditions. Patients predisposed to QTc prolongation presenting for surgery constitute a challenging group, since many drugs commonly used for PONV management will put them at risk for perioperative serious arrhythmias. This is an important topic, and our mini-review is an attempt to highlight the problem, summarize the existing experience, and generate recommendations for safe management of PONV for patients, who are at increased risk of QTc prolongation and arrhythmias. Focused prospective studies will help to find definitive answers to the discussed problems and challenges and develop specific guidelines for clinical application.
The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
Authors: Bai Guo-liang , Hu Kai-li , Huan Yi , Wang Xing , Lei Lei , Zhang Meng , Guo Chun-yan , Chang Hong-sheng , Zhao Li-bo , Liu Jing , Shen Zhu-fang , Wang Xiao-ling , Ni Xin
Published: January 2021

The purpose of this study was to explore the effect of the traditional Chinese medicine Fuyou formula on precocious puberty (PP). The Fy formula may exert an effect in female rats with PP and GT-7 cells through the GPR54/GnRH signaling pathway. To confirm the effect of the Fy formula on PP through the GPR54/GnRH signaling pathway, we first treated GT1-7 cells with the Fy formula and observed changes in the expression of related genes and proteins and in GnRH secretion. Then, we randomly divided young female Sprague-Dawley rats into the control group, model group, leuprorelin group and the Fy formula group. A PP model was established by injection of danazol on postnatal day 5, and the Fy formula was administered on PND15. The time of vaginal opening, the wet weights of the ovary and uterus, serum hormone levels and the expression of hypothalamic-related genes were observed. We found that the Fy formula delayed vaginal opening, decreased the wet weights and coefficients of the ovary and uterus, decreased the levels of serum hormones (E2, follicle-stimulating hormone and luteinizing hormone) and the cellular GnRH level, and downregulated the gene expression of Kiss1, GPR54 and GnRH in the hypothalamus and the gene and protein expression of GPR54 and GnRH in GT1-7 cells. In conclusion, the Fy formula may alleviate PP via the GPR54/GnRH signaling pathway.
Determination of Polyphenols in Ilex kudingcha and Insect Tea (Leaves Altered by Animals) by Ultra-high-performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (UHPLC-QqQ-MS) and Comparison of Their Anti-Aging Effects
Authors: Mu Jianfei , Yang Fuping , Tan Fang , Zhou Xianrong , Pan Yanni , Long Xingyao , Zhao Xin
Published: January 2021

Ilex kudingcha C.J. Tseng tea and insect tea, as traditional Chinese teas, are favored for their original craftsmanship, unique flavor, and biological functionality. In this study, ultra high-performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ-MS) was used to analyze the bioactive components of the extracts of Ilex kudingcha and insect tea, and D-galactose-induced aging mice were used to compare the in vivo anti-aging effects of Ilex kudingcha and insect tea extracts. The results were remarkable, UHPLC-QqQ-MS analysis showed that ITP contains 29 ingredients, while IKDCP contains 26 ingredients. However, due to the large differences in the content of the main chemical components in IKDCP and ITP, the effects are equally different. At the same time, the in vivo research results suggesting that the anti-aging effects of IKDCP and ITP (500 mg/kg) include the regulation of viscera indices of major organs; improvement in liver, skin, and spleen tissue morphology; decreased production of inflammatory cytokines; up regulation of SOD, CAT, GSH, GSH-PX, and T-AOC and down regulation of NO and MDA levels in serum and liver tissue; reductions in the concentration of pro-inflammatory factors, and increases in the concentration of anti-inflammatory factor. RT-qPCR and western blot assay also showed that IKDCP and ITP affect anti-aging by regulating the gene and protein expression of GSH-PX, GSH1, SOD1, SOD2, and CAT. The overall results indicate that ITP is more effective in treating oxidative damage in aging mice induced by D-galactose. Thus, ITP appears to be an effective functional drink owing to its rich nutritional components and anti-aging activities.
The Impact of Cannabidiol on Human Brain Function: A Systematic Review
Authors: Batalla Albert , Bos Julian , Postma Amber , Bossong Matthijs G.
Published: January 2021

Background: Accumulating evidence suggests that the non-intoxicating cannabinoid compound cannabidiol (CBD) may have antipsychotic and anxiolytic properties, and thus may be a promising new agent in the treatment of psychotic and anxiety disorders. However, the neurobiological substrates underlying the potential therapeutic effects of CBD are still unclear. The aim of this systematic review is to provide a detailed and up-to-date systematic literature overview of neuroimaging studies that investigated the acute impact of CBD on human brain function.

Methods: Papers published until May 2020 were included from PubMed following a comprehensive search strategy and pre-determined set of criteria for article selection. We included studies that examined the effects of CBD on brain function of healthy volunteers and individuals diagnosed with a psychiatric disorder, comprising both the effects of CBD alone as well as in direct comparison to those induced by ∆9-tetrahydrocannabinol (THC), the main psychoactive component of Cannabis.

Results: One-ninety four studies were identified, of which 17 met inclusion criteria. All studies investigated the acute effects of CBD on brain function during resting state or in the context of cognitive tasks. In healthy volunteers, acute CBD enhanced fronto-striatal resting state connectivity, both compared to placebo and THC. Furthermore, CBD modulated brain activity and had opposite effects when compared to THC following task-specific patterns during various cognitive paradigms, such as emotional processing (fronto-temporal), verbal memory (fronto-striatal), response inhibition (fronto-limbic-striatal), and auditory/visual processing (temporo-occipital). In individuals at clinical high risk for psychosis and patients with established psychosis, acute CBD showed intermediate brain activity compared to placebo and healthy controls during cognitive task performance. CBD modulated resting limbic activity in subjects with anxiety and metabolite levels in patients with autism spectrum disorders.

Conclusion: Neuroimaging studies have shown that acute CBD induces significant alterations in brain activity and connectivity patterns during resting state and performance of cognitive tasks in both healthy volunteers and patients with a psychiatric disorder. This included modulation of functional networks relevant for psychiatric disorders, possibly reflecting CBD’s therapeutic effects. Future studies should consider replication of findings and enlarge the inclusion of psychiatric patients, combining longer-term CBD treatment with neuroimaging assessments.
Transporter Gene Regulation in Sandwich Cultured Human Hepatocytes Through the Activation of Constitutive Androstane Receptor (CAR) or Aryl Hydrocarbon Receptor (AhR)
Authors: Niu Congrong , Smith Bill , Lai Yurong
Published: January 2021

The induction potentials of ligand-activated nuclear receptors on metabolizing enzyme genes are routinely tested for new chemical entities. However, regulations of drug transporter genes by the nuclear receptor ligands are underappreciated, especially in differentiated human hepatocyte cultures. In this study, gene induction by the ligands of constitutive androstane receptor (CAR) and aryl hydrocarbon receptor (AhR) was characterized in sandwich-cultured human hepatocytes (SCHH) from multiple donors. The cells were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), omeprazole (OP), 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO) and phenobarbital (PB) for three days. RNA samples were analyzed by qRT-PCR method. As expected, CITCO, the direct activator, and PB, the indirect activator of CAR, induced CYP3A4 (31 and 40-fold), CYP2B6 (24 and 28-fold) and UGT1A1 (2.9 and 4.2-fold), respectively. Conversely, TCDD and OP, the activators of AhR, induced CYP1A1 (38 and 37-fold), and UGT1A1 (4.3 and 5.0-fold), respectively. In addition, OP but not TCDD induced CY3A4 by about 61-fold. Twenty-four hepatic drug transporter genes were characterized, and of those, SLC51B was induced the most by PB and OP by about 3.3 and 6.5 fold, respectively. Marginal inductions (about 2-fold) of SLC47A1 and SLCO4C1 genes by PB, and ABCG2 gene by TCDD were observed. In contrast, SLC10A1 gene was suppressed about 2-fold by TCDD and CITCO. While clinical relevance of SLC51B gene induction or SLC10A1 gene suppression warrants further investigation, the results verified that the assessment of transporter gene inductions are not required for new drug entities, when a drug does not remarkably induce metabolizing enzyme genes by CAR and AhR activation.
Mitochondrial Iron Overload-Mediated Inhibition of Nrf2-HO-1/GPX4 Assisted ALI-Induced Nephrotoxicity
Authors: Deng Hui-Fang , Yue Lan-Xin , Wang Ning-Ning , Zhou Yong-Qiang , Zhou Wei , Liu Xian , Ni Yu-Hao , Huang Cong-Shu , Qiu Li-Zhen , Liu Hong , Tan Hong-Ling , Tang Xiang-Lin , Wang Yu-Guang , Ma Zeng-Chun , Gao Yue
Published: January 2021

Aristolactam I (ALI) is an active component derived from some Traditional Chinese medicines (TCMs), and also the important metabolite of aristolochic acid. Long-term administration of medicine-containing ALI was reported to be related to aristolochic acid nephropathy (AAN), which was attributed to ALI-induced nephrotoxicity. However, the toxic mechanism of action involved is still unclear. Recently, pathogenic ferroptosis mediated lipid peroxidation was demonstrated to cause kidney injury. Therefore, this study explored the role of ferroptosis induced by mitochondrial iron overload in ALI-induced nephrotoxicity, aiming to identify the possible toxic mechanism of ALI-induced chronic nephropathy. Our results showed that ALI inhibited HK-2 cell activity in a dose-dependent manner and significantly suppressed glutathione (GSH) levels, accompanying by significant increases in intracellular 4-hydroxynonenal (4-HNE) and intracellular iron ions. Moreover, the ALI-mediated cytotoxicity could be reversed by deferoxamine mesylate (DFO). Compared with other inhibitors, Ferrostatin-1 (Fer-1), a ferroptosis inhibitor, obviously alleviated ALI-induced cytotoxicity. Furthermore, we have shown that ALI could remarkably increase the levels of superoxide anion and ferrous ions in mitochondria, and induce mitochondrial damage and condensed mitochondrial membrane density, the morphological characteristics of ferroptosis, all of which could be reversed by DFO. Interestingly, ALI dose-dependently inhibited these protein contents of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and glutathione peroxidase 4 (GPX4), which could be partly rescued by Tin-protoporphyrin IX (SnPP) and mitoTEMPO co-treatment. In conclusion, our results demonstrated that mitochondrial iron overload-mediated antioxidant system inhibition would assist ALI-induced ferroptosis in renal tubular epithelial cells, and Nrf2-HO-1/GPX4 antioxidative system could be an important intervention target to prevent medicine containing ALI-induced nephropathy.
Elevated CO2 Levels Delay Skeletal Muscle Repair by Increasing Fatty Acid Oxidation
Authors: Ceco Ermelinda , Celli Diego , Weinberg Samuel , Shigemura Masahiko , Welch Lynn C. , Volpe Lena , Chandel Navdeep S. , Bharat Ankit , Lecuona Emilia , Sznajder Jacob I.
Published: January 2021

Muscle dysfunction often occurs in patients with chronic obstructive pulmonary diseases (COPD) and affects ventilatory and non-ventilatory skeletal muscles. We have previously reported that hypercapnia (elevated CO2 levels) causes muscle atrophy through the activation of the AMPKα2-FoxO3a-MuRF1 pathway. In the present study, we investigated the effect of normoxic hypercapnia on skeletal muscle regeneration. We found that mouse C2C12 myoblasts exposed to elevated CO2 levels had decreased fusion index compared to myoblasts exposed to normal CO2. Metabolic analyses of C2C12 myoblasts exposed to high CO2 showed increased oxidative phosphorylation due to increased fatty acid oxidation. We utilized the cardiotoxin-induced muscle injury model in mice exposed to normoxia and 10% CO2 for 21 days and observed that muscle regeneration was delayed. High CO2-delayed differentiation in both mouse C2C12 myoblasts and skeletal muscle after injury and was restored to control levels when cells or mice were treated with a carnitine palmitoyltransfearse-1 (CPT1) inhibitor. Taken together, our data suggest that hypercapnia leads to changes in the metabolic activity of skeletal muscle cells, which results in impaired muscle regeneration and recovery after injury.
The History of Domestication and Selection of Lucerne: A New Perspective From the Genetic Diversity for Seed Germination in Response to Temperature and Scarification
Authors: Ghaleb Wagdi , Ahmed Lina Qadir , Escobar-Gutiérrez Abraham J. , Julier Bernadette
Published: January 2021

Lucerne (Medicago sativa), a major perennial pasture legume, belongs to a species complex that includes several subspecies with wild and cultivated populations. Stand establishment may be compromised by poor germination. Seed scarification, deterioration and temperature have an impact on germination. The objective of this study was to analyse the genetic diversity of lucerne germination in response to three factors: (1) temperature, with seven constant temperatures ranging from 5 to 40°C, was tested on 38 accessions, (2) seed scarification was tested on the same accessions at 5 and 22°C, (3) seed deterioration was tested on two accessions and two seed lots at the seven temperatures. The germination dynamics of seed lots over time was modelled and three parameters were analysed: germinability (germination capacity), maximum germination rate (maximum% of seeds germinating per time unit), and lag time before the first seed germinates. Seed scarification enhanced germinability at both temperatures and its effect was much higher on falcata and wild sativa accessions. Incomplete loss of the hardseededness trait during domestication and selection is hypothesised, indicating that the introduction of wild material in breeding programmes should be followed by the selection for germinability without scarification. Seed lots with altered germinability had low germination at extreme temperatures, both cold and hot, suggesting that mild temperatures are required to promote germination of damaged seed lots. A large genetic diversity was revealed for germination (both capacity and rate) in response to temperature. All accessions had an optimal germination at 15 or 22°C and a poor germination at 40°C. The sativa varieties and landraces had a high germination from 5 to 34°C while the germination of falcata and the wild sativa accessions were weakened at 5 or 34°C, respectively. These differences are interpreted in terms of adaptation to the climate of their geographical origin regions in order to escape frost or heat/drought risks. These new findings give insights on adaptation and domestication of lucerne in its wide geographic area. They suggest further improvement of germination is needed, especially when introducing wild material in breeding pools to remove scarification requirements and to limit differences in response to temperature.
Language-Related Skills in Bilingual Children With Specific Learning Disorders
Authors: Riva Anna , Musetti Alessandro , Bomba Monica , Milani Lorenzo , Montrasi Valentina , Nacinovich Renata
Published: January 2021

Purpose: The purpose of this study is to better understand the characteristics of the language-related skills of bilingual children with specific learning disorders (SLD). The aim is achieved by analyzing language-related skills in a sample of bilingual (Italian plus another language) and Italian monolingual children, with and without SLD.

Patients and methods: A total of 72 minors aged between 9 and 11 were recruited and divided into four groups: 18 Italian monolingual children with SLD, 18 bilingual children with SLD, 18 Italian monolingual children without SLD, and 18 bilingual children without SLD. Each child underwent tests to evaluate different aspects of language skills: lexical and grammar, metalanguage and executive functions.

Results: With regard to lexical and grammatical skills, the conditions of SLD and bilingualism both impact naming in terms of total number of errors for words with low frequency of use, while the condition of SLD has an effect on semantic errors for words with low frequency of use. The condition of bilingualism impacts on the total errors for words with high frequency of use and on circumlocution-type errors for words with low frequency of use. There were significant effects of bilingualism and SLD on the metalinguistic test for understanding implicit meaning, and an impact of SLD on phonological awareness was also found.

Conclusion: The results suggest that both SLD and bilingualism have an effect on some lexical skills, in particular for words with low frequency of use. Both conditions, bilingualism and SLD, seem to impact on metalinguistic abilities that depend on lexical knowledge. These findings reinforce the importance of improving understanding of the neuropsychological profile of bilingual children with SLD.
Does the League Table Lie? The Development and Validation of the Perceived Performance in Team Sports Questionnaire (PPTSQ)
Authors: Gershgoren Lael , Blatt Asaf , Sela Tal , Tenenbaum Gershon
Published: January 2021

Objective performance measures are vastly used in sport psychology despite their inherent limitations (e.g., unaccounted baseline differences). Founded on the nature of group goals in team sports, we aimed at developing the Perceived Performance in Team Sports Questionnaire (PPTSQ) to capture the team members’ perception of their team’s performance. Accordingly, three dimensions were hypothesized:effort investment, skills execution, andperceived outcome. To measure these dimensions, items were generated to address the players’ perception of their team performance as a whole. Four samples of athletes were used to test the psychometric properties of the PPTSQ: professional (n= 231), collegiate (n= 222), professional—retest (n= 89), and mixed professional–collegiate (n= 139). Exploratory and confirmatory factor analyses were used to estimate construct and content validities. These procedures revealed a better data fit to a two-dimensional model that consists of effort investment and perceived outcome. The reliability analyses for the PPTSQ provide satisfactory evidence that the questionnaire is a reliable measure of perceived performance in team sport. Adequate internal consistency emerged for both dimensions (0.75 < ω < 0.89). Furthermore, a high correlation was obtained for temporal stability. Concurrent validity was addressed by correlating the PPTSQ scores with the Group Environment Questionnaire and the Team Assessment Diagnostic Instrument. Correlational analysis between the PPTSQ and an objective measure of performance was used to test its predictive validity. The correlations strongly support the concurrent and predictive validities of the PPTSQ. We conclude that our perceived performance questionnaire can address various objective measures shortcomings (e.g., considering base-rate biases) resulting in a more meaningful team performance metric. Implication of the PPTSQ for sport psychology research and applied work enhancement are discussed in detail.
The Essence of Authenticity
Authors: Dammann Olaf , Friederichs Katja M. , Lebedinski Sabine , Liesenfeld Kerstin M.
Published: January 2021

In this paper, we build upon the model of authenticity proposed by Lehman and colleagues, which includes the dimensions consistency, conformity, and connection. We expand this “3C-view” by adding a fourth dimension, continuity, which results in what we have come to call “4C-view of authenticity.” We discuss our proposal from a process perspective and emphasize that congruence might be a reasonable candidate for a concept that unifies the four dimensions of authenticity.
Patient Satisfaction With Medications for Opioid Use Disorder Treatment via Telemedicine: Brief Literature Review and Development of a New Assessment
Authors: Cole Thomas O. , Robinson Darlene , Kelley-Freeman Andrea , Gandhi Devang , Greenblatt Aaron D. , Weintraub Eric , Belcher Annabelle M.
Published: January 2021

Telemedicine is increasingly being used to treat patients with opioid use disorder (OUD). It has particular value in rural areas of the United States impacted by the opioid crisis as these areas have a shortage of trained addiction medicine providers. Patient satisfaction significantly impacts positive clinical outcomes in OUD treatment and thus is of great clinical interest. Yet little is known regarding patient satisfaction with the increasingly important platform of telemedicine-delivered medications for opioid use disorder (tMOUD). The goal of this review is to provide a summary of the existing literature regarding patient satisfaction with tMOUD. We also submit a novel survey based on an existing framework designed to assess tMOUD satisfaction, and present pilot data (N = 14) acquired from patients engaged in rural tMOUD care. Telemedicine provides a feasible method for delivering MOUD in rural areas, and our survey provides a useful assessment to measure patient satisfaction with tMOUD. In light of the pressing need for innovative and technology-driven solutions to the opioid epidemic (especially in light of the COVID-19 pandemic), future research should focus on the development and refinement of tools to assess the important implementation goal of patient satisfaction.
Gender, Populism, and the QAnon Conspiracy Movement
Authors: Bracewell Lorna
Published: January 2021

According to one recent review of the burgeoning interdisciplinary scholarly literature on populism, populism’s “relationship with gender issues remains largely understudied” (Abi-Hassan, 2017, 426–427). Of those scholarly treatments that do exist, the lion’s share focus on the role of men and masculinity in populist movements. In this essay, I argue scholarly reflection on the relationship of gender and populism should not be limited to this narrow frame. Through a close examination of the complex gender politics of QAnon, a pro-Trump conspiracy movement that burst into the mainstream of U.S. politics and culture with the onset of the global Coronavirus pandemic, I demonstrate that populist deployments of femininity are as rich, complex, and potent as their deployments of masculinity. QAnon, I argue, is a case study in how femininity, particularly feminine identities centered on motherhood and maternal duty, can be mobilized to engage women in populist political projects. Until scholars of populism start asking Cynthia Enloe’s famous question, “Where are the women?,” in a sustained and rigorous way, phenomena that are integral to populism’s functioning will elude us and our understanding of the relationship between gender and populism will remain partial and incomplete (Enloe, 2014).
Sprint Specificity of Isolated Hamstring-Strengthening Exercises in Terms of Muscle Activity and Force Production
Authors: Prince Caroline , Morin Jean-Benoît , Mendiguchia Jurdan , Lahti Johan , Guex Kenny , Edouard Pascal , Samozino Pierre
Published: January 2021

To train hamstring muscle specifically to sprint, strengthening programs should target exercises associated with horizontal force production and high levels of hamstring activity. Therefore, the objectives of this study were to analyze the correlation between force production capacities during sprinting and hamstring strengthening exercises, and to compare hamstring muscle activity during sprinting and these exercises. Fourteen track and field regional level athletes performed two maximal 50-m sprints and six strengthening exercises: Nordic hamstring exercises without and with hip flexion, Upright-hip-extension in isometric and concentric modalities, Standing kick, and Slide-leg-bridge. The sprinting horizontal force production capacity at low (F0) and high (V0) speeds was computed from running velocity data. Hamstring muscle performances were assessed directly or indirectly during isolated exercises. Hamstring muscle electromyographic activity was recorded during all tasks. Our results demonstrate substantially large to very large correlations between V0 and performances in the Upright-hip-extension in isometric (rs = 0.56; p = 0.040), Nordic hamstring exercise without hip flexion (rs = 0.66; p = 0.012) and with 90° hip flexion (rs = 0.73; p = 0.003), and between F0 and Upright-hip-extension in isometric (rs = 0.60; p = 0.028) and the Nordic hamstring exercise without hip flexion (rs = 0.59; p = 0.030). However, none of the test exercises activated hamstring muscles more than an average of 60% of the maximal activation during top-speed sprinting. In conclusion, training programs aiming to be sprint-specific in terms of horizontal force production could include exercises such as the Upright-hip-extension and the Nordic hamstring exercise, in addition to maximal sprinting activity, which is the only exercise leading to high levels of hamstring muscle activity.
Fluids of the Future
Authors: Edwards Thomas H. , Hoareau Guillaume L.
Published: January 2021

Fluids are a vital tool in the armament of acute care clinicians in both civilian and military resuscitation. We now better understand complications from inappropriate resuscitation with currently available fluids; however, fluid resuscitation undeniably remains a life-saving intervention. Military research has driven the most significant advances in the field of fluid resuscitation and is currently leading the search for the fluids of the future. The veterinary community, much like our civilian human counterparts, should expect the fluid of the future to be the fruit of military research. The fluids of the future not only are expected to improve patient outcomes but also be field expedient. Those fluids should be compatible with military environments or natural disaster environments. For decades, military personnel and disaster responders have faced the peculiar demands of austere environments, prolonged field care, and delayed evacuation. Large scale natural disasters present field limitations often similar to those encountered in the battlefield. The fluids of the future should, therefore, have a long shelf-life, a small footprint, and be resistant to large temperature swings, for instance. Traumatic brain injury and hemorrhagic shock are the leading causes of preventable death for military casualties and a significant burden in civilian populations. The military and civilian health systems are focusing efforts on field-expedient fluids that will be specifically relevant for the management of those conditions. Fluids are expected to be compatible with blood products, increase oxygen-carrying capabilities, promote hemostasis, and be easy to administer in the prehospital setting, to match the broad spectrum of current acute care challenges, such as sepsis and severe systemic inflammation. This article will review historical military and civilian contributions to current resuscitation strategies, describe the expectations for the fluids of the future, and describe select ongoing research efforts with a review of current animal data.
Synthesis and Experimental Characterization of a MWCNT-Filled Bio-Based Adhesive
Authors: Tserpes Konstantinos , Tzatzadakis Vasileios
Published: January 2021

In the present paper, a novel epichlorohydrin/cardanol adhesive was reinforced by multi-walled carbon nanotubes (MWCNTs) and characterized experimentally. The adhesive was reinforced by MWCNTs in weight ratios (wt %) of 0.5%, 1.0% and 2.0%. The bulk properties of the reinforced adhesive were characterized through dynamic mechanical analysis tests, tension tests, and fracture toughness tests, while its shear behavior was characterized through single-lap shear tests on aluminum and composite bonded specimens. The morphology of the reinforced adhesive was characterized using scanning electron microscopy tests. Due to the high viscosity of the bio-based adhesive, special efforts were placed on the dispersion of the MWCNTs into the adhesive, which was achieved through mechanical mixing. The results from the tests show that the presence of the MWCNTs increases the glass transition temperature, the Young’s modulus and the fracture toughness of the reinforced bio-based adhesive, while it decreases its tensile strength. This contradictory finding is attributed to the formation of MWCNT agglomerates into the adhesive. For the content of 2.0 wt %, the shear strength of the reinforced adhesive is increased by 57% for the aluminum joints and by 10.4% for the composite joints. The findings of the study reveal that the reinforcement of the bio-based adhesive by MWCNTs is feasible from a manufacturing viewpoint and may increase the efficiency of the adhesive in structural applications.
Response of Spring Wheat (Triticum aestivum) to Deficit Irrigation Management under the Semi-Arid Environment of Egypt: Field and Modeling Study
Authors: Ouda Samiha , Noreldin Tahany , Alarcón Juan José , Ragab Ragab , Caruso Gianluca , Sekara Agnieszka , Abdelhamid Magdi T.
Published: January 2021

In many areas of the world, water shortages prevail and threaten food production. Deficit irrigation was commonly investigated in dry areas as a precious and sustainable production approach. Using the CropSyst model to simulate the effects of different deficit irrigation treatments could help draw conclusions and save time, effort, and money. Therefore, the aims of this research were (i) to calibrate and validate the CropSyst model for wheat under different sustained and phenological stage-based deficit irrigation treatments, (ii) to simulate the impacts of the latter treatments on limiting wheat yield reduction. Two field experiments were conducted in Nubaria (Egypt), representing an arid environment. They included seven irrigation treatments: (1) 100%, (2) 75%, or (3) 50% of crop evapotranspiration (ETc) during the whole crop cycle; (4) 50% ETc at tillering only, or (5) at booting only, or (6) at grain filling only, or (7) at both tillering and grain filling, with the replenishment of 100% ETc to the treatments (4) to (7) in the remaining phenological stages. The results revealed that phenological stage-based deficit irrigation of wheat resulted in lower yield reduction compared to sustained deficit irrigation treatments, with a 6% yield reduction when 50% ETc was applied at the booting stage. Wheat yield loss was reduced to 4 or 6% when 95 or 90% of ETc were applied, respectively. The CropSyst model accurately simulated wheat grain and total dry matter under deficit irrigation with low RMSE value. In conclusion, the CropSyst model can be reliably used for evaluating the strategy of planned deficit irrigation management in terms of wheat production under the arid environment.
Musculoskeletal Growth Modulation in Gilthead Sea Bream Juveniles Reared at High Water Temperature and Fed with Palm and Rapeseed Oils-Based Diets
Authors: Balbuena-Pecino Sara , Riera-Heredia Natàlia , Gasch-Navalón Esther , Sánchez-Moya Albert , Fontanillas Ramon , Gutiérrez Joaquim , Navarro Isabel , Capilla Encarnación
Published: January 2021

The upward trend of seawater temperature has encouraged improving the knowledge of its consequences on fish, considering also the development of diets including vegetable ingredients as an approach to achieve a more sustainable aquaculture. This study aims to determine the effects on musculoskeletal growth of: (1) a high-water temperature of 28 °C (versus 21 °C) in gilthead sea bream juveniles (Sparus aurata) fed with a diet rich in palm oil and, (2) feeding the fish reared at 28 °C with two other diets containing rapeseed oil or an equilibrated combination of both vegetable oils. Somatic parameters and mRNA levels of growth hormone-insulin-like growth factors (GH-IGFs) axis-, osteogenic-, myogenic-, lipid metabolism- and oxidative stress-related genes in vertebra bone and/or white muscle are analyzed. Overall, the data indicate that high-water rearing temperature in this species leads to different adjustments through modulating the gene expression of members of the GH-IGFs axis (down-regulating igf-1, its receptors, and binding proteins) and also, to bone turnover (reducing the resorption-activity genes cathepsin K (ctsk) and matrix metalloproteinase-9 (mmp9)) to achieve harmonic musculoskeletal growth. Moreover, the combination of palm and rapeseed oils seems to be the most beneficial at high-water rearing temperature for both balanced somatic growth and muscular fatty acid uptake and oxidation.
Effects of Whole Corn Germ, a Source of Linoleic Acid, on Carcass Characteristics and Meat Quality of Feedlot Lambs
Authors: Nascimento Camila O. , Pina Douglas S. , Cirne Luís G. A. , Santos Stefanie A. , Araújo Maria L. G. M. L. , Rodrigues Thomaz C. G. C. , Silva William P. , Souza Mateus N. S. , Alba Henry D. R. , de Carvalho Gleidson G. P.
Published: January 2021

The whole corn germ (WCG), due to its desirable nutritional characteristics, has been studied as feed for ruminants. This study aimed to evaluate the effects of WCG inclusion as a linoleic acid source in diets for feedlot lambs on carcass characteristics, physicochemical composition, sensory attributes, and fatty acid profile of the meat. Forty non-castrated, crossbreed Dorper x Santa Inês lambs were distributed in a completely randomized design to evaluate the inclusion levels (0, 30, 60, 90, and 120 g/kg dry matter (DM)) of whole corn germ (WCG) in the diet. The dietary inclusion of WCG did not influence (p > 0.05) the weight gain and carcass characteristics, with the exception of the subcutaneous fat thickness (p < 0.01), which was higher in animals fed diets with higher levels of WCG. Lightness (L *; p = 0.04), yellowness (b *; p < 0.01), shear force (p = 0.04), linoleic fatty acid concentrations (p = 0.03), and total polyunsaturated fatty acids (p = 0.04) had a quadratic increase due to WCG inclusion in the diets. The use of up to 120 g/kg DM of WCG in lamb diets does not affect the carcass characteristics, physicochemical composition, and sensory attributes of the meat. Despite this, the best polyunsaturated fatty acid profile in lambs’ meat is obtained using 76.7 g/kg DM of WCG.
Overflow Energy Loss Recovery System Based on Hydraulic Motor-Electric Generator
Authors: Li Zhongshen , Su Ling , Lin Tianliang
Published: January 2021

Overflow loss is one of the main reasons for the inefficiency of the hydraulic system. Aiming at the overflow loss in the hydraulic system, an energy recovery system based on a hydraulic motor and generator is proposed. Further, a pressure monitoring and control strategy based on variable speed control for electric generator is adopted, which can control the pressure differential between the inlet and outlet of the proportional relief valve as a small value, and achieve the purpose of energy recovery by reducing the loss of the pressure differential between the inlet and outlet of the relief valve. Through establishing a simulation system model and test platform, the stability, step response characteristics and energy saving of the system are studied. The results show that the energy recovery unit at the outlet of the relief valve does not affect the normal operation of the relief valve, but also greatly reduces the pressure regulation deviation. Besides, the efficiency of overflow loss recovery system about is 67%.
Thermoplastic Polymeric Materials for Spacecraft Applications: Flame Retardant Properties and UV/AtOx Aging Analysis
Authors: Pastore Roberto , Albano Marta , Delfini Andrea , Santoni Fabio , Marchetti Mario
Published: January 2021

Space-mission development introduced the problem of human isolation in extreme environments. The integration of architectural concepts such as windows, with their technical implications into human space missions, is necessary especially for long-duration space flights. Such solutions must be subjected to close certification testing in order to establish their compliance with severe space environment conditions. Moreover, projects of long-term missions involve a renewed concern about fire safety in manned space vehicles. The supposed occupancy time of the order of decades, in fact, makes unplanned fire ignition events virtually unavoidable. An experimental test-plan performed to qualify a commercial thermoplastic material for applications as transparent element in spacecraft is reported in the present work. A flame exposure test, as well as ultraviolet radiation and atomic oxygen erosion rate measurements, was carried out on a commercial poly-sulfone material, following ASTM standard procedures. The aim of the research was to develop and put forward flame retardant structures able to withstand the harsh space environment, preventing or mitigating the degradation of their physical and chemical integrity, with particular regard to the visible-light transparency. The results obtained show that the tested poly-sulfone may be considered as a promising material for the claimed application, even if further surface optimization treatments should be conceived in order to gain a full adaptability to the operative constraints.
The Influence of the Modernization of the City Sewage System on the External Load and Trophic State of the Kartuzy Lake Complex
Authors: Grochowska Jolanta , Tandyrak Renata
Published: January 2021

A study was carried out in the Kartuzy lake complex, which has been a receiver of raw domestic sewage since the 1950s. In 2018, the city’s sewage system of Kartuzy was modernized. An analysis of the water quality prior to the modernization of the sewage system revealed that the total phosphorus (TP) load that was introduced to the individual lakes from external sources substantially exceeded the dangerous load concentration (defined by Vollenweider) that causes accelerated eutrophication. The annual TP load introduced to the analyzed lakes in 2017 exceeded the critical load by 200% (Mielenko) to 1000% (Klasztorne Duże). Protective measures reduced the external loading of nutrients. In the case of Mielenko Lake, a 37% decrease in the external TP load was noted, and also a 32% decrease in the external TP load in Karczemne Lake, a 66% decrease in Klasztorne Małe Lake and a 54% decrease in Klasztorne Duże Lake was noted. The protective measures resulted in a slight decrease in the concentrations of phosphorus and nitrogen in the water. However, these changes did not improve the environmental conditions in the lakes. In a situation where the internal fertilization process in the lakes has started, the improvement of water quality will only be possible through restoration efforts with methods adjusted to the individual characteristics of each lake.
Acknowledgment to Reviewers of Axioms in 2020
Authors:   Axioms Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Axioms maintains its standards for the high quality of its published papers [...]
Coffee Label Assessment Using Sensory and Biometric Analysis of Self-Isolating Panelists through Videoconference
Authors: Gonzalez Viejo Claudia , Zhang Hanyan , Khamly Annabel , Xing Yunjia , Fuentes Sigfredo
Published: January 2021

Label concepts, information, logos, figures, and colors of beverages are critical for consumer perception, preference, and purchase intention. This is especially relevant for new beverage products. During social isolation, many sensory laboratories were unable to provide services, making virtual sensory sessions relevant to studying different label concepts and design preferences among consumers. This study proposed a novel virtual sensory system to analyze coffee labels using videoconference, self-reported, and biometric analysis software from video recordings to obtain sensory and emotional responses from 69 participants (power analysis: 1 − β > 0.99) using six different label concepts: (i) fun, (ii) bold, (iii) natural, (iv) everyday, (v) classic, and (vi) premium. The results show that the label concept rated as having the highest perceived quality was premium, presenting significant differences (p < 0.05) compared to all of the other concepts. The least perceived quality score was attributed to the bold concept due to the confronting aroma lexicon (cheese dip), which is supported by previous studies. Furthermore, even though graphics, colors, and the product name can be considered positive attributes, they do not determine perceived quality or purchase intention, which was found for the bold, everyday, and classic concepts. The findings from this study were as expected and are consistent with those from similar publications related to labels, which shows that the proposed virtual method for sensory sessions and biometrics is reliable. Further technology has been proposed to use this system with multiple participants, which could help beverage companies perform virtual sensory analysis of new products’ labels.
Navigating the Collagen Jungle: The Biomedical Potential of Fiber Organization in Cancer
Authors: Ouellette Jonathan N. , Drifka Cole R. , Pointer Kelli B. , Liu Yuming , Lieberthal Tyler J , Kao W John , Kuo John S. , Loeffler Agnes G. , Eliceiri Kevin W.
Published: January 2021

Recent research has highlighted the importance of key tumor microenvironment features, notably the collagen-rich extracellular matrix (ECM) in characterizing tumor invasion and progression. This led to great interest from both basic researchers and clinicians, including pathologists, to include collagen fiber evaluation as part of the investigation of cancer development and progression. Fibrillar collagen is the most abundant in the normal extracellular matrix, and was revealed to be upregulated in many cancers. Recent studies suggested an emerging theme across multiple cancer types in which specific collagen fiber organization patterns differ between benign and malignant tissue and also appear to be associated with disease stage, prognosis, treatment response, and other clinical features. There is great potential for developing image-based collagen fiber biomarkers for clinical applications, but its adoption in standard clinical practice is dependent on further translational and clinical evaluations. Here, we offer a comprehensive review of the current literature of fibrillar collagen structure and organization as a candidate cancer biomarker, and new perspectives on the challenges and next steps for researchers and clinicians seeking to exploit this information in biomedical research and clinical workflows.
Function and Fiber-Type Specific Distribution of Hsp60 and αB-Crystallin in Skeletal Muscles: Role of Physical Exercise
Authors: D’Amico Daniela , Fiore Roberto , Caporossi Daniela , Di Felice Valentina , Cappello Francesco , Dimauro Ivan , Barone Rosario
Published: January 2021

Skeletal muscle is a plastic and complex tissue, rich in proteins that are subject to continuous rearrangements. Skeletal muscle homeostasis can be affected by different types of stresses, including physical activity, a physiological stressor able to stimulate a robust increase in different heat shock proteins (HSPs). The modulation of these proteins appears to be fundamental in facilitating the cellular remodeling processes related to the phenomenon of training adaptations such as hypertrophy, increased oxidative capacity, and mitochondrial activity. Among the HSPs, a special attention needs to be devoted to Hsp60 and αB-crystallin (CRYAB), proteins constitutively expressed in the skeletal muscle, where their specific features could be highly relevant in understanding the impact of different volumes of training regimes on myofiber types and in explaining the complex picture of exercise-induced mechanical strain and damaging conditions on fiber population. This knowledge could lead to a better personalization of training protocols with an optimal non-harmful workload in populations of individuals with different needs and healthy status. Here, we introduce for the first time to the reader these peculiar HSPs from the perspective of exercise response, highlighting the control of their expression, biological function, and specific distribution within skeletal muscle fiber-types.
Autophagy: Mechanisms and Therapeutic Potential of Flavonoids in Cancer
Authors: Pang Xuening , Zhang Xiaoyi , Jiang Yuhuan , Su Quanzhong , Li Qun , Li Zichao
Published: January 2021

Autophagy, which is a conserved biological process and essential mechanism in maintaining homeostasis and metabolic balance, enables cells to degrade cytoplasmic constituents through lysosomes, recycle nutrients, and survive during starvation. Autophagy exerts an anticarcinogenic role in normal cells and inhibits the malignant transformation of cells. On the other hand, aberrations in autophagy are involved in gene derangements, cell metabolism, the process of tumor immune surveillance, invasion and metastasis, and tumor drug-resistance. Therefore, autophagy-targeted drugs may function as anti-tumor agents. Accumulating evidence suggests that flavonoids have anticarcinogenic properties, including those relating to cellular proliferation inhibition, the induction of apoptosis, autophagy, necrosis, cell cycle arrest, senescence, the impairment of cell migration, invasion, tumor angiogenesis, and the reduction of multidrug resistance in tumor cells. Flavonoids, which are a group of natural polyphenolic compounds characterized by multiple targets that participate in multiple pathways, have been widely studied in different models for autophagy modulation. However, flavonoid-induced autophagy commonly interacts with other mechanisms, comprehensively influencing the anticancer effect. Accordingly, targeted autophagy may become the core mechanism of flavonoids in the treatment of tumors. This paper reviews the flavonoid-induced autophagy of tumor cells and their interaction with other mechanisms, so as to provide a comprehensive and in-depth account on how flavonoids exert tumor-suppressive effects through autophagy.
Acknowledgment to Reviewers of BioTech in 2020
Authors:   BioTech Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that BioTech maintains its standards for the high quality of its published papers [...]