bluuebunny/crossref_metadata_2025 huggingface dataset:
A replicable strategy for mapping air pollution’s community-level health impacts and catalyzing prevention
Authors: Landrigan Philip J. , Fisher Samantha , Kenny Maureen E. , Gedeon Brittney , Bryan Luke , Mu Jenna , Bellinger David
Published: July 2022

Background

Air pollution was responsible for an estimated 6.7 million deaths globally in 2019 and 197,000 deaths in the United States. Fossil fuel combustion is the major source.

Hypothesis

Mapping air pollution’s health impacts at the community level using publicly available data and open-source software will provide a replicable strategy for catalyzing pollution prevention.

Methods

Using EPA’s Environmental Benefits Mapping and Analysis (BenMAP-CE) software and state data, we quantified the effects of airborne fine particulate matter (PM2.5) pollution on disease, death and children’s cognitive function (IQ Loss) in each city and town in Massachusetts. To develop a first-order estimate of PM2.5 pollution’s impact on child IQ, we derived a concentration-response coefficient through literature review.

Findings

The annual mean PM2.5 concentration in Massachusetts in 2019 was 6.3 μg/M3, a level below EPA’s standard of 12 μg/M3 and above WHO’s guideline of 5 μg/M3. In adults, PM2.5 pollution was responsible for an estimated 2780 (Confidence Interval [CI] 2726 – 2853) deaths: 1677 (CI, 1346 – 1926) from cardiovascular disease, 2185 (CI, 941–3409) from lung cancer, 200 (CI, 66–316) from stroke, and 343 (CI, 222–458) from chronic respiratory disease. In children, PM2.5 pollution was responsible for 308 (CI, 105–471) low-weight births, 15,386 (CJ, 5433-23,483) asthma cases, and a provisionally estimated loss of nearly 2 million Performance IQ points; IQ loss impairs children’s school performance, reduces graduation rates and decreases lifetime earnings. Air-pollution-related disease, death and IQ loss were most severe in low-income, minority communities, but occurred in every city and town in Massachusetts regardless of location, demographics or median family income.

Conclusion

Disease, death and IQ loss occur at air pollution exposure levels below current EPA standards. Prevention of disease and premature death and preservation of children’s cognitive function will require that EPA air quality standards be tightened. Enduring prevention will require government-incentivized transition to renewable energy coupled with phase-outs of subsidies and tax breaks for fossil fuels. Highly localized information on air pollution’s impacts on health and on children’s cognitive function has potential to catalyze pollution prevention.
Clinical significance of tumor-infiltrating lymphocytes investigated using routine H&E slides in small cell lung cancer
Authors: Zhou Guangrun , Zheng Jifang , Chen Zhiwei , Hu Dan , Li Suyu , Zhuang Wu , He Zhiyong , Lin Gen , Wu Biao , Zhang Wei , Fang Weimin , Zheng Fei , Wang Jiezhong , Chen Gang , Chen Mingqiu
Published: July 2022

Background

Tumor-infiltrating lymphocytes (TILs), investigated using routine hematoxylin and eosin (H&E)-stained section slides (H&E-sTILs), provide a robust prognostic biomarker in various types of solid cancer. The purpose of the present study was to investigate the prognostic significance of H&E-sTILs in patients with small cell lung cancer (SCLC).

Methods

The clinical data of patients with SCLC who had been treated in our cancer center between January 2013 and October 2019 were collected and retrospectively reviewed. The H&E-sTILs were re-assessed by two experienced pathologists independently. H&E-sTILs that affected the overall survival (OS), progression free survival (PFS) and brain-metastasis free survival (BMFS) rates were explored using the Kaplan–Meier method, and the log-rank test was used to assess the differences. Multivariate analysis was subsequently performed using the Cox proportion hazards model.

Results

A total of 159 patients with SCLC who fulfilled the inclusion criteria were enrolled in the current study. The OS rates at 1, 2 and 3 years were 59.8, 28.6 and 19.8%, respectively, for the whole group. The 3-year OS, PFS and BMFS rates for the H&E-sTILs(+) and H&E-sTILs(−) groups were 25.1% cf. 5.1% (P = 0.030), 14.0% cf. 4.0% (P = 0.013), and 66.0% cf. 11.4% (P = 0.023), respectively. Multivariate analyses subsequently revealed that H&E-sTILs, clinical M stage, the cycles of chemotherapy and short-term response to thoracic radiotherapy were independent factors affecting OS, whereas H&E-sTILs, clinical N stage, clinical M stage and short-term response to chemotherapy were factors affecting PFS. The H&E-sTILs affected OS, PFS and BMFS simultaneously.

Conclusions

The results of this retrospective study have shown that H&E-sTILs may be considered as a prognostic biomarker affecting the short-term response to treatment, and they are the one and only risk factor for BMFS. However, due to the limitations of the nature of the retrospective design and shortcomings in visually assessing the TILs based on the H&E-stained slides, further prospective studies are required to confirm these conclusions.
Donepezil ameliorates Aβ pathology but not tau pathology in 5xFAD mice
Authors: Choi Hee-Jeong , Park Jin-Hee , Jeong Yoo Joo , Hwang Jeong-Woo , Lee Soojung , Lee Heeyong , Seol Eunyoung , Kim Ik-whi , Cha Byung-Yoon , Seo Jinsoo , Moon Minho , Hoe Hyang-Sook
Published: July 2022

The cholinesterase inhibitor donepezil is used to improve Aβ pathology and cognitive function in patients with Alzheimer’s disease (AD). However, the impact of donepezil on tau pathology is unclear. Thus, we examined the effects of donepezil on Aβ and tau pathology in 5xFAD mice (a model of AD) in this study. We found that intraperitoneal injection of donepezil (1 mg/kg, i.p.) exhibited significant reductions in Aβ plaque number in the cortex and hippocampal DG region. In addition, donepezil treatment (1 mg/kg, i.p.) reduced Aβ-mediated microglial and, to a lesser extent, astrocytic activation in 5xFAD mice. However, neither intraperitoneal/oral injection of donepezil nor oral injection of rivastigmine altered tau phosphorylation at Thr212/Ser214 (AT100), Thr396, and Thr231 in 5xFAD mice. Surprisingly, we observed that intraperitoneal/oral injection of donepezil treatment significantly increased tau phosphorylation at Thr212 in 5xFAD mice. Taken together, these data suggest that intraperitoneal injection of donepezil suppresses Aβ pathology but not tau pathology in 5xFAD mice.
The effect of gestational age, low birth weight and parity on birth asphyxia among neonates in sub-Saharan Africa: systematic review and meta-analysis: 2021
Authors: Techane Masresha Asmare , Alemu Tewodros Getaneh , Wubneh Chalachew Adugna , Belay Getaneh Mulualem , Tamir Tadesse Tarik , Muhye Addis Bilal , Kassie Destaye Guadie , Wondim Amare , Terefe Bewuketu , Tarekegn Bethelihem Tigabu , Ali Mohammed Seid , Fentie Beletech , Gonete Almaz Tefera , Tekeba Berhan , Kassa Selam Fisiha , Desta Bogale Kassahun , Ayele Amare Demsie , Dessie Melkamu Tilahun , Atalell Kendalem Asmare , Assimamaw Nega Tezera
Published: July 2022

Background

Despite simple and proven cost-effective measures were available to prevent birth asphyxia; studies suggested that there has been limited progress in preventing birth asphyxia even in healthy full-term neonates. In Sub-Saharan Africa, Inconsistency of magnitude of birth asphyxia and its association gestational age, Low birth Weight and Parity among different studies has been observed through time.

Objective

This study aimed to estimate the Pooled magnitude of birth asphyxia and its association with gestational age, Low birth Weight and Parity among Neonates in Sub-Saharan Africa.

Method

PubMed, Cochrane library and Google scholar databases were searched for relevant literatures. In addition, reference lists of included studies were retrieved to obtain birth asphyxia related articles. Appropriate search term was established and used to retrieve studies from databases. Searching was limited to cohort, cross-sectional, and case-control studies conducted in Sub-Saharan africa and published in English language. Joanna Briggs Institute Meta-Analysis of Statistics Assessment and Review Instrument (JBI-MAStARI) was used for critical appraisal of studies. Heterogeneity across the included studies was evaluated by using the inconsistency index (I2) test. Funnel plot and the Egger’s regression test were used to test publication bias. A weighted inverse variance random effects- model was used to estimate the pooled prevalence of birth asphyxia among neonates in Sub-Saharan Africa. STATA™ version 11softwarewasused to conduct the meta-analysis.

Result

A total of 40 studies with 176,334 study participants were included in this systematic review and meta-analysis. The overall pooled magnitude of birth asphyxia in Sub-Saharan Africa was 17.28% (95% CI; (15.5, 19.04). low birth weight (AOR = 2.58(95% CI: 1.36, 4.88)), primigravida (AOR = 1.15 (95% CI: 0.84, 1.46) andMeconium-stained amniotic fluid (AOR = 6(95% CI: 3.69, 9.74)) werevariables significantly associated with the pooled prevalence of birth asphyxia.

Conclusion

The pooled magnitude of birth asphyxia was found to be high in Sub-Saharan Africa. Low birthweight and Meconium-stained amniotic fluid were variables significantly associated with birth asphyxia in Sub-Saharan Africa. Hence, it is better to develop early detection and management strategies for the affected neonates with low birth weight and born from mothers intrapartum meconium stained amniotic fluid.
Systematic review of Plasmodium knowlesi in Indonesia: a risk of emergence in the context of capital relocation to Borneo?
Authors: Bin Said Ibrahim , Kouakou Yobouet Ines , Omorou Roukayatou , Bienvenu Anne-Lise , Ahmed Kamruddin , Culleton Richard , Picot Stephane
Published: July 2022

Background

The Indonesian Republic plans to relocate its capital from Jakarta to East Kalimantan, Borneo Island, in the next few years. This relocation may be associated with deforestation, decreased biodiversity, and an increased risk of emerging zoonotic infections, including Plasmodium knowlesi malaria. The Malaysian part of Borneo Island is one of the main hotspots of P. knowlesi malaria.

Methods

Considering this risk, we evaluated the transmission dynamics of P. knowlesi in the Indonesian Archipelago based on a literature search and extensive review of data from the Indonesian Ministry of Health.

Results

We report that 545 P. knowlesi cases were documented in Indonesia, mainly in the Aceh and North Sumatra provinces, with 95% of these occurring in the last 4 years.

Conclusions

The main P. knowlesi vectors are present in the area of the future capital, requiring strengthened surveillance to reduce the risk of emerging cases in a rapidly growing population.
Maternal SMCHD1 controls both imprinted Xist expression and imprinted X chromosome inactivation
Authors: Wanigasuriya Iromi , Kinkel Sarah A. , Beck Tamara , Roper Ellise A. , Breslin Kelsey , Lee Heather J. , Keniry Andrew , Ritchie Matthew E. , Blewitt Marnie E. , Gouil Quentin
Published: July 2022

Embryonic development is dependent on the maternal supply of proteins through the oocyte, including factors setting up the adequate epigenetic patterning of the zygotic genome. We previously reported that one such factor is the epigenetic repressor SMCHD1, whose maternal supply controls autosomal imprinted expression in mouse preimplantation embryos and mid-gestation placenta. In mouse preimplantation embryos, X chromosome inactivation is also an imprinted process. Combining genomics and imaging, we show that maternal SMCHD1 is required not only for the imprinted expression ofXistin preimplantation embryos, but also for the efficient silencing of the inactive X in both the preimplantation embryo and mid-gestation placenta. These results expand the role of SMCHD1 in enforcing the silencing of Polycomb targets. The inability of zygotic SMCHD1 to fully restore imprinted X inactivation further points to maternal SMCHD1’s role in setting up the appropriate chromatin environment during preimplantation development, a critical window of epigenetic remodelling.
Systemic lupus erythematosus with trisomy X: a case report and review of the literature
Authors: Luo Fang , Ye Qiao , Shen Jie
Published: July 2022

Background

The cause of systemic lupus erythematosus is not completely clear so far, but the prevalence of systemic lupus erythematosus is significantly increased in people with additional X chromosomes.

Case presentation

We report a 17-year-old Chinese female patient with systemic lupus erythematosus complicated with trisomy X, accompanied by lupus nephritis, pancytopenia, hemolytic anemia, and multiserous effusion. The patient recovered well after treatment and returned regularly. We review the previously reported cases to summarize the clinical characteristics of these patients.

Conclusion

The additional X chromosome is related to the development of systemic lupus erythematosus. Whether it is a subtype of systemic lupus erythematosus remains to be further confirmed.
Extracellular matrix derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF
Authors: Ma Beilei , Wang Tengkai , Li Juan , Wang Qian
Published: July 2022

Background

Angiogenesis is required in many physiological conditions, including bone regeneration, wound healing, and tissue regeneration. Mesenchymal stem cells-derived extracellular matrix (MSCs-ECM) could guide intricate cellular and tissue processes such as homeostasis, healing and regeneration.

Methods

The purpose of this study is to explore the effect and mechanism of ECM derived from decellularized Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) on endothelial cell viability and angiogenesis. The human umbilical vein endothelial cells (HUVECs) were pretreated with WJ-MSCs ECM for 2d/7d/14d, respectively. After pretreatment, the angiogenesis ability of HUVECs was detected.

Results

In this study, we found for the first time that WJ-MSCs ECM could improve the angiogenesis ability of HUVECs with a time-dependent manner in vitro. Mechanically, WJ-MSCs ECM activated the focal adhesion kinase (FAK)/P38 signaling pathway via integrin αVβ3, which further promoted the expression of the cellular (c)-Myc. Further, c-Myc increased histone acetylation levels of the vascular endothelial growth factor (VEGF) promoter by recruiting P300, which ultimately promoting VEGF expression.

Conclusions

ECM derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF.This study is expected to provide a new approach to promote angiogenesis in bone and tissue regeneration.
Oxidative stress-induced endothelial cells-derived exosomes accelerate skin flap survival through Lnc NEAT1-mediated promotion of endothelial progenitor cell function
Authors: Guo Linlin , Chen Yuxuan , Feng Xiaoling , Sun Di , Sun Jiaming , Mou Shan , Zhao Kangcheng , An Ran
Published: July 2022

Background

Flap transplantation is commonly used in reconstructive surgery. A prerequisite for skin flap survival is sufficient blood supply. However, such approaches remain unclear. This study aimed to explore the underlying mechanisms of exosomes derived from human umbilical vascular endothelial cells (HUVECs) exposed to oxidative stress on endothelial progenitor cells (EPCs) and their subsequent influence on the survival of skin flaps.

Methods

HUVECs were treated with various concentrations of H2O2to establish an oxidative stress model. To investigate the effects of H2O2-HUVEC-Exos and HUVEC-Exos, Cell Counting Kit-8, tube formation, invasion assays, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed in EPCs. Microarray analysis was used to reveal the differentially expressed long non-coding RNAs (lncRNAs) in the H2O2-HUVEC-Exos and HUVEC-Exos. In addition, gene silencing and western blotting were employed to determine the mechanism behind lncRNA nuclear enrichment enriched transcript 1 (Lnc NEAT1) in EPCs. Further, a rat skin flap model was used to determine the role of the exosomes in skin flap survival in vivo.

Results

HUVECs were stimulated with 100 μmol/L H2O2for 12 h to establish an oxidative stress model. H2O2-HUVEC-Exos promoted the proliferation, tube formation, and invasion of EPCs and remarkably increased skin flap survival compared to the HUVEC-Exos and control groups. Sequencing of exosome RNAs revealed that the Lnc NEAT1 level was dramatically increased in the H2O2-HUVEC-Exos, leading to activation of the Wnt/β-catenin signaling pathway. Comparatively, knockdown of Lnc NEAT1 in HUVEC-Exos and H2O2-HUVEC-Exos significantly inhibits the angiogenic capacity of EPCs, reduced the survival area of skin flap and downregulated the expression levels of Wnt/β-catenin signaling pathway proteins, whereas Wnt agonist partly reversed the negative effect of NEAT1 downregulation on EPCs through the Wnt/β-catenin signaling pathway.

Conclusions

Exosomes derived from HUVECs stimulated by oxidative stress significantly promoted the pro-angiogenic ability of EPCs through the Wnt/β-catenin signaling pathway mediated by Lnc NEAT1 and hence enhanced random flap survival in vivo. Therefore, the application of H2O2-HUVEC-Exos may serve as an alternative therapy for improving random skin flap survival.
Integrating criticality concepts into road network disruption assessments for volcanic eruptions
Authors: Hayes Josh L. , Biass Sébastien , Jenkins Susanna F. , Meredith Elinor S. , Williams George T.
Published: July 2022

Road networks in volcanically active regions can be exposed to various volcanic hazards from multiple volcanoes. Exposure assessments are often used in these environments to prioritise risk management and mitigation efforts towards volcanoes or hazards that present the greatest threat. Typically, road exposure has been assessed by quantifying the amount of road network affected by different hazards and/or hazard intensity. Whilst this approach is computationally efficient, it largely fails to consider the relative importance of road segments within the network (i.e., road criticality). However, road criticality is an important indicator of the disruption that may be caused by an eruption. In this work, we aim to integrate road criticality concepts to enhance typical volcanic eruption road exposure assessments into road disruption assessments. We use three key components to quantify disruption: a) road criticality, b) impact severity, and c) affected road quantity. Two case study eruptions: Merapi 2010 and Kelud 2014, both in Java, Indonesia, are used to demonstrate the usefulness of integrating road criticality into road disruption assessments from volcanic eruptions. We found that disruption of the road network from the Kelud 2014 case study was an order of magnitude greater than the Merapi 2010 case study. This is primarily driven by the more widely dispersed tephra fall from the Kelud 2014 event, which affected nearly 28% of Java’s road network length, compared to Merapi 2010, which affected 1.5%. We also identified potential disruption hotspots that were affected by both of these case study eruptions. At Merapi, roads that carry traffic directly away from the summit, those that cross major valleys, and the major Yogyakarta-Magelang highway were key disruption hotspots, which has implications for moving large volumes of traffic efficiently, such as in an evacuation. The Kelud case study highlighted the potential impacts of widespread tephra falls on socio-economic activity and connectivity of large urban centres. Our approach has been designed such that it can be applied entirely using open-sourced datasets. Therefore, the approach to integrating road criticality in this paper can be used, applied, and adapted to assess road network disruption at any volcano in the world.
PICF-LDA: a topic enhanced LDA with probability incremental correction factor for Web API service clustering
Authors: Shen Jiaji , Huang Wen , Hu Qiang
Published: July 2022

Web API is a popular way to organize network services in cloud computing environment. However, it is a challenge to find an appropriate service for the requestor from massive Web API services. Service clustering can improve the efficiency of service discovery for its ability of reducing search space. Latent Dirichlet Allocation (LDA) is the most frequently used topic model in service clustering. To further improve the topic representation ability of LDA, we propose a new variant model of LDA with probability incremental correction factor (PICF-LDA) to generate the high-quality service representation vectors (SRVs) for Web API services. We first compute the words’ topic contribution degree (TCD) in the service description text by its context weight and part-of-speech (POS) weight. Then the probability incremental correction factor (PICF) for a word is designed based on TCD and the word’s maximum topic probability value. PICF is used to correct the probability distributions in SRVs. Experiments show that PICF-LDA has a better performance than LDA, the variant LDA models and other state-of-the-art topic models in service clustering.
Outcomes of laparoscopic versus open total gastrectomy with D2 lymphadenectomy for gastric cancer: a systematic review and meta-analysis
Authors: Yang Yongpu , Chen Yuyan , Hu Yilin , Feng Ying , Mao Qinsheng , Xue Wanjiang
Published: July 2022

Background

The effectiveness of laparoscopic total gastrectomy with D2 lymphadenectomy (LTGD2) remains controversial. This meta-analysis compares surgical and survival outcomes of LTGD2 and open total gastrectomy with D2 lymphadenectomy (OTGD2) for gastric cancer.

Methods

Controlled studies comparing LTGD2 and OTGD2 published before November 2021 were retrieved via database searches. We compared intraoperative outcomes, pathological data, postoperative outcomes, 5-year disease-free survival (DFS), and overall survival (OS).

Results

17 studies were included, containing 4742 patients. Compared with OTGD2, the LTGD2 group had less blood loss (mean difference [MD] = − 122.48; 95% CI: − 187.60, − 57.37; P = 0.0002), fewer analgesic medication (MD = -2.48; 95% CI: − 2.69, − 2.27; P < 0.00001), earlier first flatus (MD = − 1.03; 95% CI: − 1.80, − 0.26; P = 0.009), earlier initial food intake (MD = − 0.89; 95% CI: − 1.09, − 0.68; P < 0.00001) and shorter hospital stay (MD = − 3.24; 95% CI: − 3.75, − 2.73; P < 0.00001). The LTGD2 group had lower postoperative total complication ratio (OR = 0.76; 95% CI: 0.62, 0.92; P = 0.006), incision (OR = 0.50; 95% CI:0.31, 0.79; P = 0.003) and pulmonary (OR = 0.57; 95% CI: 0.34, 0.96; P = 0.03) complication rates, but similar rates of other complications and mortality. Total number of dissected lymph nodes were similar, but the number of No. 10 dissected nodes was less with LTGD2 (MD = − 0.31; 95% CI: − 0.46, − 0.16; P < 0.0001). There was no difference in 5-year OS (P = 0.19) and DFS (P = 0.34) between LTGD2 and OTGD2 groups.

Conclusions

LTGD2 produces small trauma, fast postoperative recovery and small length of hospital stays than OTGD2, and had similar long-term clinical efficacy as OTGD2. However, these results still need further high-quality prospective randomized controlled trials confirmation.
Ketogenic diets slow melanoma growth in vivo regardless of tumor genetics and metabolic plasticity
Authors: Weber Daniela D. , Aminzadeh-Gohari Sepideh , Thapa Maheshwor , Redtenbacher Anna-Sophia , Catalano Luca , Capelôa Tânia , Vazeille Thibaut , Emberger Michael , Felder Thomas K. , Feichtinger René G. , Koelblinger Peter , Dallmann Guido , Sonveaux Pierre , Lang Roland , Kofler Barbara
Published: July 2022

Background

Growing evidence supports the use of low-carbohydrate/high-fat ketogenic diets as an adjunctive cancer therapy. However, it is unclear which genetic, metabolic, or immunological factors contribute to the beneficial effect of ketogenic diets. Therefore, we investigated the effect of ketogenic diets on the progression and metabolism of genetically and metabolically heterogeneous melanoma xenografts, as well as on the development of melanoma metastases in mice with a functional immune system.

Methods

Mice bearing BRAF mutant, NRAS mutant, and wild-type melanoma xenografts as well as mice bearing highly metastatic melanoma allografts were fed with a control diet or ketogenic diets, differing in their triglyceride composition, to evaluate the effect of ketogenic diets on tumor growth and metastasis. We performed an in-depth targeted metabolomics analysis in plasma and xenografts to elucidate potential antitumor mechanisms in vivo.

Results

We show that ketogenic diets effectively reduced tumor growth in immunocompromised mice bearing genetically and metabolically heterogeneous human melanoma xenografts. Furthermore, the ketogenic diets exerted a metastasis-reducing effect in the immunocompetent syngeneic melanoma mouse model. Targeted analysis of plasma and tumor metabolomes revealed that ketogenic diets induced distinct changes in amino acid metabolism. Interestingly, ketogenic diets reduced the levels of alpha-amino adipic acid, a biomarker of cancer, in circulation to levels observed in tumor-free mice. Additionally, alpha-amino adipic acid was reduced in xenografts by ketogenic diets. Moreover, the ketogenic diets increased sphingomyelin levels in plasma and the hydroxylation of sphingomyelins and acylcarnitines in tumors.

Conclusions

Ketogenic diets induced antitumor effects toward melanoma regardless of the tumors´ genetic background, its metabolic signature, and the host immune status. Moreover, ketogenic diets simultaneously affected multiple metabolic pathways to create an unfavorable environment for melanoma cell proliferation, supporting their potential as a complementary nutritional approach to melanoma therapy.
Assessment of vitamin D among male adolescents and young adults hospitalized with eating disorders
Authors: Nagata Jason M. , Grandis Anna , Bojorquez-Ramirez Paola , Nguyen Anthony , Downey Amanda E. , Ganson Kyle T. , Patel Khushi P. , Machen Vanessa I. , Buckelew Sara M. , Garber Andrea K.
Published: July 2022

Purpose

Medical complications of eating disorders in males are understudied compared to females, as is the case of vitamin D deficiency. The aim of this study was to assess vitamin D levels among male and female adolescents and young adults hospitalized for medical complications of eating disorders.

Methods

We retrospectively reviewed electronic medical records of patients aged 9–25 years (N = 565) admitted to the University of California, San Francisco Eating Disorders Program for medical instability, between May 2012 and August 2020. Serum vitamin D (25-hydroxy) level was assessed at admission as was history of prior calcium, vitamin D, or multivitamin supplementation. Linear regression was used to assess factors associated with vitamin D levels.

Results

A total of 93 males and 472 females met eligibility criteria (age 15.5 ± 2.8, 58.8% anorexia nervosa; admission body mass index 17.6 ± 2.91). Among male participants, 44.1% had 25-hydroxyvitamin D levels < 30 ng/mL, 18.3% had 25-hydroxyvitamin D levels < 20 ng/mL, and 8.6% had 25-hydroxyvitamin D levels < 12 ng/mL. There were no significant differences in 25-hydroxyvitamin D levels in males compared to females, except that a lower proportion (1.9%) of female participants had 25-hydroxyvitamin D levels < 12 ng/mL (p = 0.001). Only 3.2% of males reported calcium or vitamin D-specific supplementation prior to hospital admission, while 8.6% reported taking multivitamins. White race, prior calcium/vitamin D supplementation, and higher calcium levels were associated with higher vitamin D levels on admission.

Conclusions

Nearly half of patients admitted to the hospital for malnutrition secondary to eating disorders presented with low 25-hydroxyvitamin D levels; males were more likely than females to have severe vitamin D deficiency. These findings support vitamin D assessment as part of the routine medical/nutritional evaluation for hospitalized eating disorder patients, with particular attention on male populations.
Protective effects of coconut water against the intraperitoneal infused carbon tetrachloride-induced toxicity—evaluations of biochemical, haematological and histopathological profiles in rats
Authors: Emmanuel Okezie , Elekwa Ifeanyichukwu , Paul-Joseph Chidiebere , Ude Victor C. , Egedeuzu Ozioma G. , Ijioma Solomon N. , Amachaghi Victor Obioma , Uche-Ikonne Chikezie , Ugbogu Eziuche A.
Published: July 2022

Background

Coconut water is a relished beverage traditionally used as a remedy for childhood diarrhoea and gastroenteritis. It can be given to an individual who has hangover or suspected to have ingested a toxic substance. In this study, we evaluated the protective effect of coconut water against carbon tetrachloride (CCl4)-induced toxicity in rats.

Results

Administration of coconut water decreased significantly albumin, aspartate transaminase (AST), alanine transaminase (ALT), urea, creatinine, bicarbonate (HCO3−), total cholesterol, triglycerides, low-density lipoprotein, very low-density lipoprotein and malondialdehyde (MDA) levels compared to the non-pretreated group. Furthermore, high-density lipoprotein, glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) increased significantly (p < 0.05) in coconut water-pretreated groups compared to the negative control. There were no observed pathological changes in the coconut water-pretreated groups, but slight enlargement of the central veins and tubules was evident when compared to the negative control.

Conclusions

Therefore, intake of coconut water may be protective against toxicity induced by CCl4 as its pretreatment elevated antioxidant parameters (GSH, SOD and CAT) and did not alter biochemical parameters in rats.
The small GTPase BcSec4 is involved in conidiophore development, membrane integrity, and autophagy in Botrytis cinerea
Authors: Wang Guanbo , Zou Jian , Wang Yadi , Liang Wenxing , Li Delong
Published: July 2022

Small GTPases of the Rab family play important roles in membrane trafficking and autophagy. Previous studies have shown that the small GTPase Sec4 is involved in vegetative growth, protein secretion, and pathogenicity of fungal phytopathogens. In this study, the Rab GTPase BcSec4, an ortholog of the yeast Sec4p protein, was characterized in Botrytis cinerea. The ΔBcSEC4 mutant produces many abnormal conidiophores; it is thus defective in conidiation and displays attenuated pathogenicity. Notably, the germination and penetration of conidia are unaffected in the ΔBcSEC4 mutant. Further studies showed that the secretion of extracellular proteins was altered in the ΔBcSEC4 mutant, and the mutant showed impaired autophagy and defects in membrane integrity. Taken together, these results suggest that BcSec4 is critical for conidiophore development, membrane integrity, pathogenicity, and autophagy.
Properties of neighborhood for certain classes associated with complex order and m-q-p-valent functions with higher order
Authors: Madian Samar
Published: July 2022

In this paper, by using q-calculus (Jackson’s q-derivative) $$D_{q,p}$$

D

q
,
p

we defined new operator $$D_{\lambda ,q,p}^{n}f^{(m)}(z)$$

D

λ
,
q
,
p

n

f

(
m
)

(
z
)

. After that, we used this operator to introduce two new subclasses of multivalent analytic functions with complex order. Also, we obtained coefficients estimates and consequent inclusion relationships involving the $$N_{j,\delta ,m}^{p,q}(f)$$

N

j
,
δ
,
m

p
,
q

(
f
)

-neighborhood of these classes
Effects of prebiotics in combination with probiotics on intestinal hydrolase activity, microbial population and immunological biomarkers in SD rats fed an AIN-93G diet
Authors: Kim Min-Jeong , Jeon Dong-Gyeong , Lim Yong , Jang Insurk
Published: July 2022

Background

Gastrointestinal microbiota, which comprises hundreds of different types of microbes, biologically plays crucial roles in the host’s health. Probiotics (PRO) did not always have a positive benefit on the host, depending on strains of microbes and the physiochemical properties of prebiotics (PRE), indicating that the properties of PRE in combination with PRO might have different effects on the gut ecology. The aim of this study was to assess the effects of insoluble or soluble PRE with PRO on intestinal digestive hydrolase, the fecal microbes, and immunological biomarkers in SD rats fed an AIN-93G diet.

Results

Forty, 8-week-old SD rats were randomly assigned to 4 groups with 10 replicates in each; cellulose (CELL), cellulose + probiotics (CELPRO), oatmeal (OATS), and oatmeal + probiotics (OATPRO) groups. After 4-week feeding trial, rats were treated with saline or lipopolysaccharide (LPS, 1 mg/kg) to examine the alleviating effects of PRO and PRE on immunological responses. There was a significant (p < 0.05) decrease in feed intake of rats fed the oatmeal supplemented diet without affecting growth performance. Blood triglyceride was significantly (p < 0.05) decreased in rats fed the oatmeal diet, and aspartate aminotransferase (AST) was significantly (p < 0.05) decreased in rats fed the PRO supplemented diet. Intestinal maltase, sucrose, and lactase activities were significantly (p < 0.05) higher in rats fed PRO compared with rats not fed PRO. Rats fed the oatmeal showed a significant (p < 0.01) increase in the fecal colony forming units (CFU) of Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae compared with those fed cellulose. LPS-treated rats fed PRO showed a significant (p < 0.05) increase in blood secretory immunoglobulin A (sIgA) compared with those not fed PRO. The LPS-treated rats fed PRO resulted in decreased (p < 0.05) blood IL-6 compared with those not fed PRO, indicating that a dietary PRO alleviated inflammatory response in LPS-treated rats.

Conclusions

Dietary oatmeal increased fecal microbes, and PRO supplement resulted in increased intestinal hydrolase and immune functions of the host, demonstrating that soluble PRE with supplemented with PRO could be a more bioactive combination of synbiotics in SD rats.
Comparative prevalence of different types of viral hepatitis in the district Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan
Authors: Khan Muhammad Ashraf
Published: July 2022

Background

Viral hepatitis causes both liver inflammation and damage and is a serious health problem.

Methods

The indoor data of different types of hepatitis were collected from the official records of the administration of District Head Quarter Hospital Dera Ismail Khan for 2 years (2020–2021).

Results

A total of 1193 cases of viral hepatitis during the study period were found including viral hepatitis C accounted for 547 (45.9%) cases, followed by the co-infection of viral hepatitis A and E 367 (30.8%) cases, and viral hepatitis B 279 (23.4%) cases. March showed the highest prevalence of hepatitis (33.2%), followed by February (10.2%). The co-infection of hepatitis A and E showed a relatively higher prevalence in 6 months from May to November except September including a peak in June (76.5%) during the study period. While viral hepatitis B demonstrated a comparatively higher percentage prevalence in both February and September with a peak in February (68%), and viral hepatitis C is dominant in January, March, and December with a peak in March (83.8%).

Conclusion

All types of viral hepatitis showed variation in prevalence over months and monthly variation concerning peak prevalence exists among different types of hepatitis. Both viral hepatitis A and E demonstrated similar trend in relative prevalence in both 2020 and 2021. Nevertheless, viral hepatitis A and B showed monthly and seasonal variation in relatively prevalence in both years. The study help in adopting strategies for the prevention of viral hepatitis in the study area.
Individual and national financial impacts of informal caring for people with mental illness in Australia, projected to 2030
Authors: Schofield Deborah , Zeppel Melanie J. B. , Tanton Robert , Veerman Jacob Lennert , Kelly Simon J. , Passey Megan E. , Shrestha Rupendra N.
Published: July 2022

Background

Mental illness has a significant impact not only on patients, but also on their carers’ capacity to work.

Aims

To estimate the costs associated with lost labour force participation due to the provision of informal care for people with mental illness in Australia, such as income loss for carers and lost tax revenue and increased welfare payments for government, from 2015 to 2030.

Method

The output data of a microsimulation model Care&WorkMOD were analysed to project the financial costs of informal care for people with mental illness, from 2015 to 2030. Care&WorkMOD is a population-representative microsimulation model of the Australian population aged between 15 and 64 years, built using the Australian Bureau of Statistics Surveys of Disability, Ageing and Carers data and the data from other population-representative microsimulation models.

Results

The total annual national loss of income for all carers due to caring for someone with mental illness was projected to rise from AU$451 million (£219.6 million) in 2015 to AU$645 million (£314 million) in 2030 in real terms. For the government, the total annual lost tax revenue was projected to rise from AU$121 million (£58.9 million) in 2015 to AU$170 million (£82.8 million) in 2030 and welfare payments to increase from AU$170 million (£82.8 million) to AU$220 million (£107 million) in 2030.

Conclusions

The costs associated with lost labour force participation due to the provision of informal care for people with mental illness are projected to increase for both carers and government, with a widening income gap between informal carers and employed non-carers, putting carers at risk of increased inequality.
Advance Care Planning in Patients With Metastatic Cancer: A Quality Improvement Initiative
Authors: Oppenheim Steven , Figlin Robert A. , Seferian Edward G. , Reed Margaret , Irwin Scott A. , Rosen Bradley T.
Published: July 2022

PURPOSE:

An initiative aimed to increase the rate of advance care planning (ACP) activities for outpatients with metastatic cancer, an essential step to achieving goal concordant care.

METHODS:

Patients with metastatic cancer were identified by International Classification of Diseases-10 coding and later by oncologists' electronic health record documentation of metastatic tumor status. ACP activities were defined as either an ACP note, Advance Directive, Physician Orders for Life-Sustaining Therapy (POLST), or a Palliative Medicine (PM) consultation within the prior year. From 2017 to 2020, the initiative screened more than 5,000 total unique cancer patients per year. PM consultants were embedded in tumor boards, oncology care team meetings, and shared oncology clinic space. Quarterly reports were sent to 60 oncologists at three cancer care sites with data of their percentage of ACP activities for patients with metastatic cancer compared with their peers. Oncologists' identities were initially blinded, but later unblinded. Oncologists also received a monthly list of patients with metastatic cancer without ACP activities.

RESULTS:

The rate of ACP activities for patients with metastatic cancer increased from a baseline of 37% in July 2017 to 57% by the end of 2020. PM consultations increased from 12% to 39% and ACP notes increased from 16% to 29% during the same interval. There was no change in Advance Directive (17%-20%) or POLST completion (7%-6%).

CONCLUSION:

ACP activities are an essential step to achieve goal concordant care, and this initiative successfully increased ACP activities for patients with metastatic cancer. However, given that the main source of increased ACP activities during this initiative was PM referrals, further progress will depend upon strengthening the oncology care teams' ACP skills and motivation for completion.
The Missing Mortality Advantage for European Immigrants to the United States in the Early Twentieth Century
Authors: Bakhtiari Elyas
Published: July 2022

Immigrant populations typically have lower mortality rates and longer life expectancies than their nonimmigrant counterparts. This immigrant mortality advantage has been a recurrent finding in demographic and population health research focused on contemporary waves of immigration. However, historical data suggest that European immigrants to the United States in the early twentieth century had worse health and higher rates of mortality, yet it remains unclear why a mortality advantage was absent for immigrants during this period. This article combines Vital Statistics records and Lee–Carter mortality models to analyze mortality by nativity status for the U.S. White population from 1900 to 1960, examining variation by age, sex, time, and place. Contrary to contemporary expectations of a foreign-born mortality advantage, White immigrants had higher mortality rates in the early 1900s, with the largest foreign-born disadvantage among the youngest and oldest populations. Although foreign-born and U.S.-born White mortality rates trended toward convergence over time, the foreign-born mortality penalty remained into the 1950s. A decomposition analysis finds that immigrants' concentration in cities, which had higher rates of infectious disease mortality, accounted for nearly half of the nativity difference in 1900, and this place effect declined in subsequent decades. Additional evidence, such as a spike in mortality inequalities during the 1918 influenza pandemic, suggests that common explanations for the immigrant mortality advantage may be less influential in a context of high risk from infectious disease.
Respiratory syncytial virus disrupts the airway epithelial barrier by decreasing cortactin and destabilizing F-actin
Authors: Gao Nannan , Raduka Andjela , Rezaee Fariba
Published: July 2022

Respiratory syncytial virus (RSV) infection is the leading cause of acute lower respiratory tract infection in young children worldwide. Our group recently revealed that RSV infection disrupts the airway epithelial barrier in vitro and in vivo. However, the underlying molecular pathways were still elusive. Here, we report the critical roles of the filamentous actin (F-actin) network and actin-binding protein cortactin in RSV infection. We found that RSV infection causes F-actin depolymerization in 16HBE cells, and that stabilizing the F-actin network in infected cells reverses the epithelial barrier disruption. RSV infection also leads to significantly decreased cortactin in vitro and in vivo. Cortactin-knockout 16HBE cells presented barrier dysfunction, whereas overexpression of cortactin protected the epithelial barrier against RSV. The activity of Rap1 (which has Rap1A and Rap1B forms), one downstream target of cortactin, declined after RSV infection as well as in cortactin-knockout cells. Moreover, activating Rap1 attenuated RSV-induced epithelial barrier disruption. Our study proposes a key mechanism in which RSV disrupts the airway epithelial barrier via attenuating cortactin expression and destabilizing the F-actin network. The identified pathways will provide new targets for therapeutic intervention toward RSV-related disease.

This article has an associated First Person interview with the first author of the paper.
Ontogeny can provide insight into the roles of natural and sexual selection in cricket cuticular hydrocarbon evolution
Authors: Simmons Leigh W. , Lovegrove Maxine , Du Xin (Bob) , Ren Yonglin , Thomas Melissa L.
Published: July 2022

The often complex cocktails of hydrocarbon compounds found on the cuticles of insects can serve both naturally and sexually selected functions, contributing to an individual's ability to withstand water loss and attract mating partners. However, whether natural and sexual selection act synergistically or antagonistically on a species' cuticular hydrocarbon (CHC) profile remains unclear. Here, we examined the ontogeny of the CHC profile in a species of cricket, Teleogryllus oceanicus, while manipulating humidity during development. We predicted that juvenile crickets should produce only those compounds that contribute to desiccation resistance, while those compounds contributing specifically to male attractiveness should be produced only at sexual maturity. Further, if attractive CHCs come at a cost to desiccation resistance as predicted by some models of sexual selection, then males reared under low humidity should be constrained to invest less in attractive CHCs. Crickets reared under low humidity produced more long-chain methyl-branched alkanes, alkenes and alkadienes than did crickets reared under high humidity. The abundance of n-alkanes was unaffected by humidity treatment. Sexual dimorphism in the CHC profile was not apparent until adult emergence and became exaggerated 10 days after emergence, when crickets were sexually mature. Males produced more of the same compounds that were increased in both sexes under low humidity, but the humidity treatment did not interact with sex in determining CHC abundance. The data suggest that CHC profiles which protect crickets from desiccation might have synergistic effects on male attractiveness, as there was no evidence to suggest males trade-off a CHC profile produced in response to low humidity for one associated with sexual signalling.
Multiparty democracy, social cohesion, and human development in Sub-Saharan Africa: A panel data analysis
Authors: Diori Hamidou Issaka , NaRanong Anchana
Published: July 2022

Background: The reintroduction of multiparty democracy in Sub-Saharan Africa during the early 90s and the persistent poor human development performance of the region, have sparked several controversies regarding the need for such a political system in Africa. In the extant literature, these controversies have been characterized by fierce theoretical debates as to whether multiparty democracy influences human development and the scholarly contention that social cohesion, rather than this form of democracy, ameliorates human development. The present study examines these controversies along with the perception of Sub-Saharan African populations concerning the democratic transition and its impact on their well-being. Methods: A panel data analysis of 35 countries was conducted between 1995 to 2019 in order to determine the effect of multiparty democracy, social cohesion, and their interaction on human development. Fixed-effects and system generalized methods of moments estimations were used to control for specific characteristics. Results: The study found that, multiparty democracy, social cohesion, and the interaction between these two variables have a positive long-term effect on human development. More importantly, the study revealed that social cohesion decreases the negative influence of multiparty democracy on human development. Conclusions: Overall, the findings of this study suggest that multiparty democracy is likely to improve the well-being of the populations of Sub-Saharan Africa if the degree of social cohesion is sufficiently high. That also means that social cohesion is susceptible to dampening the negative effect of multiparty democracy on human development in the context of this study. As such, social cohesion is critical in terms of the extent to which multiparty democracy wields influence on human development outcomes in Sub-Saharan Africa. Based on these conclusions, a number of policy recommendations are discussed in the present study towards the achievement of sustained human development in the Sub-Saharan African region.