bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of typology and reactivity in initiation and development of disadaptive disorders
Authors: Артеменков А.А.
Published: December 2020

В данном обзоре поднимается проблема переходных состояний, возникающих на грани нормы и патологии, которые принято называть пограничными психическими расстройствами (ППР). В работе высказана гипотеза о том, что в современных условиях жизни человека часто возникает дезинтеграция психической деятельности в связи со значительной информационной нагрузкой на интегративные системы головного мозга. В обобщении материала сделан акцент на описание внутренних (биологических) факторов, определяющих возникновение и развитие дезадаптивных состояний и сопровождающих их пограничных психических расстройств. Обсуждается вопрос о роли индивидуально-типологических свойств нервной системы и реактивности личности в формировании и развитии пограничной психической патологии. Рассматривается вопрос о лечебно-профилактических и коррекционных мероприятиях, направленных на снижение дезадаптивных проявлений у человека в процессе жизнедеятельности и минимизацию пограничных психических расстройств.

This review focuses on the issue of transition states that occur on the edge of normal and pathological conditions and are called borderline mental disorders (BMD). The author hypothesized that in the modern life, disintegration of mental activity frequently develops in association with a significant informational load on brain integrative systems. In summarizing the material, an emphasis was made on description of internal (biological) factors that determine the emergence and development of disadaptive conditions and concurrent BMDs. The review discusses the role of individual typological features of the nervous system and individual reactivity in the formation and development of BMD and focuses on therapeutic, preventive, and correctional measures aimed at alleviating disadaptive signs in humans and minimizing BMDs.
Comparative study of oxidant properties of oxidized and unoxidized bisretinoids of lipofuscine granules in the retinal pigment epithelium of human eye
Authors: Яковлева М.А. , Сакина Н.Л. , Кольчугина И.Б. , Арбуханова П.М. , Борзенок С.А. , Фельдман Т.Б. , Островский М.А.
Published: December 2020

Актуальность. Недавно нами было показано, что при возрастной макулярной дегенерации сетчатки наблюдается повышенное содержание продуктов фотоокисления и фотодеградации бисретиноидов по сравнению с нормой. Поэтому на сегодняшний день вопрос о фототоксичности этих продуктов становится актуальным для решения проблемы поиска путей лечения и профилактики патологии. Цель. Провести сравнительное исследование фотосенсибилизирующего действия N-ретинилиден-N-ретинилэтаноламина (А2Е) и продуктов его фотоокисления и фотодеградации на индуцированную видимым светом пероксидацию липидов фоторецепторных мембран. Материалы и методы. При помощи метода высокоэффективной жидкостной хроматографии были получены отдельные фракции неокисленных и окисленных бисретиноидов в хлороформном экстракте липофусциновых гранул из ретинального пигментного эпителия кадаверных глаз. Результаты. Проведено сравнительное исследование фототоксических свойств неокисленных и окисленных бисретиноидов липофусциновых гранул из клеток ретинального пигментного эпителия глаза человека на пероксидацию липидов наружных сегментов фоторецепторных клеток. Выводы. Окисленные бисретиноиды липофусциновых гранул менее фототоксичны по сравнению с их неокисленными формами.

Background. Recently we have shown that age-related macular degeneration is associated with higher than normal levels of bisretinoid photo-oxidation and photo-degradation products. Therefore, the issue of their phototoxicity currently becomes relevant for finding ways to treat and prevent this pathology. Aim. To conduct a comparative study of the photosensitizing effect of N-retinylidene-N-retinylethanolamine (A2E) and its photooxidation and photodegradation products on light-induced lipid peroxidation in photoreceptor membranes. Materials and methods. Using high-performance liquid chromatography fractions of unoxidized and oxidized bisretinoids were isolated in the chloroform extract of lipofuscin granules from the retinal pigment epithelium of cadaver eyes. Results. The study compared phototoxic effects of unoxidized and oxidized bisretinoids of lipofuscin granules from human retinal pigment epithelial cells on lipid peroxidation in rod outer segments. Conclusions. Oxidized bisretinoids of lipofuscin granules are less phototoxic compared to their unoxidized forms.
Effects of additional respiratory resistance in adolescents with increased sympathetic tone
Authors: Еркудов В.О. , Пуговкин А.П.
Published: December 2020

Изучены закономерности изменения вегетативного компонента регуляции деятельности сердца при включении дозированного дыхания с добавочным сопротивлением (ДДС) у подростков с исходным преобладанием тонуса симпатической нервной системы (ПТСНС). Методы. В исследовании приняли участие 17 человек, из них 8 юношей и 9 девушек, в возрасте от 14 до 17 лет. Всем добровольцам проводили регистрацию кардиоинтервалограммы с анализом вариабельности сердечного ритма (ВСР) до и во время дыхания c ДДС. Проводили анализ ВСР с выявлением вегетативного тонуса у детей с исходно ПТСНС. Результаты. Выявлено значимое снижение тонуса симпатической и возрастание тонуса парасимпатической нервной системы у детей с ПТСНС. Результаты работы позволяют прогнозировать изменения вегетативной регуляции деятельности сердца у детей в условиях закрытого помещения с длительно находящимися там людьми.

Changes in neurogenic regulation of the heart function were studied after the onset of dosed lung ventilation with artificially increased total airway resistance in 8 male and 9 female adolescents aged 14-17 with originally high tone of the sympathetic system. Analysis of cardiointervalography and heart rate variability showed a significant decrease in sympathetic tone and an increase in parasympathetic tone due to the respiration with additional resistance, which can be regarded as a model for prediction of changes in neurogenic regulation of the heart function in children after a lengthy stay in a stuffy, enclosed area.
Morphological characteristics of cellular elements in imprints of the dental implant-gum contact area
Authors: Григорьян А.С. , Орлов А.А. , Рыбалко А.С.
Published: December 2020

В работе были поставлены две задачи: изучить морфологические характеристики клеточных элементов в отпечатках/цитограммах из области контакта дентальный имплантат-десна; проанализировать эффективность морфологической характеристики клеточных элементов в цитограммах для оценки состояния тканевого комплекса этой области в целом. Методы. Исследовали морфологию клеточных элементов, содержащихся в отпечатках цитограмм из области контакта десна-дентальный имплантат, полученных в динамике в сроки с 1 по 720 сутки от 90 пациентов (из них 44 женщины и 46 мужчин, общее количество имплантатов 223). Пациентам производили дентальные имплантации трех типов: немедленную, одноэтапную и двухэтапную. Оценку состояния тканей периимплантационной зоны производили полуколичественным методом по 4-балльной оценочной шкале, в которой учитывались: проявления клеточной гибели, интенсивность колонизирования клеток микроорганизмами, а также сочетание указанных проявлений патологических реакций. Результаты. Проведён анализ морфологических элементов клеточного состава импланто-десневой борозды. Обнаружено, что балльная оценка цитопатологии была выше у пациентов с клиническими проявлениями воспаления тканей вокруг имплантатов. Заключение. На основании данных проведенного исследования сделан вывод о высокой информативности данных морфологического исследования цитограмм, отражающее состояние тканей периимплантационной зоны.

Imprints/cytograms of the dental implants-gum contact area were used for this study. Aims: To analyze morphological characteristics of cellular elements in imprints/cytograms obtained from the area of dental implants-gum contact and to evaluate the efficacy of using cell morphological characteristics of these imprints as a basis for a conclusion about the overall tissue condition in the peri-implant zone. Methods. Cytopathological changes, including dystrophic changes and signs of cells death, such as autophagy and apoptosis, were studied. Also, the study focused on leukocytes-platelet aggregation and other manifestations of cytopathology. A 4-score scale was used for assessment of the severity of cytopathological changes, including the number of cellular elements with signs of cell death (autophagy and apoptosis), intensity of microbial colonization in cells, and leukocyte-platelet aggregates. Results. Morphological elements of the cell composition in the implant-gingival sulcus were analyzed. The cytopathology score was higher for patients with clinical manifestations of inflammation in tissues around the implants. Conclusion. The study showed a high incidence of signs of autophagy cell death in the cytograms, which was closely correlated with local and systemic pathogenic factors.
Comparison of hazard and toxicity of tobacco cigarettes, electronic nicotine delivery systems, and tobacco heating systems (IQOS) (review)
Authors: Еникеев Д.А. , Идрисова Л.Т. , Еникеев О.А. , Кузнецов К.О. , Ахмадеева Д.Р. , Еникеева С.А. , Габдрахманова И.Д. , Гарифуллин А.И. , Сахабутдинов Ф.А.
Published: December 2020

В статье представлены актуальные данные экспериментальных и клинических исследований по изучению влияния различных средств доставки никотина в организм курильщика. Проведён сравнительный анализ воздействия табачных сигарет, электронных средств доставки никотина и системы нагревания табака IQOS на показатели здоровья и развития организма человека, а также лабораторных животных. Показано, что, несмотря на громкие заявления производителей альтернативных средств доставки никотина о полной безвредности их устройств, результаты экспериментальных исследований обнаруживают в них не только известные канцерогены, но и новые потенциально опасные вещества, которые не содержатся в обычных сигаретах, и влияние которых на организм достаточно не изучено. На данный момент имеются все основания полагать, что употребление электронных сигарет и IQOS может приводить к заболеваниям нервной, кроветворной, иммунной, дыхательной систем, а также к развитию онкологических заболеваний.

The article presents current results of experimental and clinical studies focusing on effects of various nicotine delivery systems. The authors conducted a comparative analysis of the impact of tobacco cigarettes, electronic nicotine delivery devices, and IQOS tobacco heating systems on the health and development of humans and laboratory animals. The review of recent literature has shown that, despite ambitious statements of the manufacturers about complete safety of their devices, experiments have demonstrated that they release not only well-known carcinogens but also new, potentially hazardous substances. These substances are not contained in ordinary cigarettes, and their effects on the body has not been sufficiently studied. Presently, it seems very likely that the use of electronic cigarettes and IQOS can lead to diseases of the nervous, hematopoietic, immune, and respiratory systems as well as to oncological diseases.
Platelet Microparticles: Formation and Properties
Authors: Кубатиев А.А. , Боровая Т.Г. , Жуховицкий В.Г. , Адреевская С.Г. , Шевлягина Н.В.
Published: December 2020

Статья является обзорной, содержит информацию об образовании и свойствах микрочастиц тромбоцитов, их роли в физиологических процессах организма и патогенезе заболеваний. Рассматриваются механизмы ремоделирования плазмолеммы и цитоскелета тромбоцитов в ходе формирования микрочастиц, приводятся фенотипические особенности микрочастиц и их значение как молекулярных трансмиттеров и активаторов сигнальных путей в клетках-мишенях. Представлена информация об участии микрочастиц в патогенезе заболеваний, сопровождающихся тромбообразованием, в регуляции ангиогенеза и метастазировании опухолей. Рассматривается влияние микрочастиц тромбоцитов на факторы воспаления и потенциальная роль микрочастиц в патогенезе инфекционных заболеваний.

This article is a review that contains information about the formation and properties of microparticles of platelets, about their role in the physiological processes of the organism and pathogenesis of the disease. Are considered: mechanisms of remodeling plasmolemma and platelet cytoskeleton during formation of microparticles, the phenotypic features of microparticles and their role as molecular transmitters and activators of signaling pathways in target cells. Presents information on the involvement of microparticles in the pathogenesis of diseases associated with thrombus formation, in the regulation of angiogenesis and metastasis of tumors. Discusses the influence of platelet microparticles on inflammation factors and the potential role of microparticles in the pathogenesis of infectious diseases.
Lipid peroxidation in rodents with exudative inflammation
Authors: Иванова Е.А. , Матюшкин А.И. , Золотов Н.Н. , Воронина Т.А.
Published: December 2020

Целью исследования является оценка уровня первичных продуктов пероксидации липидов (ПОЛ), диеновых конъюгатов (ДК), и вторичного продукта ПОЛ, малонового диальдегида (МДА), в воспалительном выпоте и сыворотке крови грызунов с экспериментальным экссудативным воспалением. Методы. Концентрацию ДК и МДА определяли спектрофотометрически в перитонеальном выпоте (ПВ) у мышей с гликогеновым перитонитом и у крыс с уксусным перитонитом, а также в сыворотке крови крыс с моделью уксусного перитонита и с моделью вызванного каррагенаном отека лапы. Результаты. В ПВ у крыс с уксусным перитонитом и мышей с гликогеновым перитонитом наблюдается увеличение концентрации МДА по сравнению с интактными животными (контроль). При этом в сыворотке крови крыс с уксусным перитонитом через 3 часа после индукции воспаления наблюдалось увеличение концентрации как МДА, так и ДК. В сыворотке крови крыс с каррагенановым отеком лапы через 5 часов после введения раствора флогогена обнаружено только достоверное повышение уровня МДА, а уровень ДК не отличался от контроля. Заключение. Полученные результаты могут представлять интерес для дальнейшего исследования динамики уровня первичных и вторичных продуктов ПОЛ в острый период воспалительных процессов для характеристики патогенеза их развития и последующей оценки влияния соединений с антиоксидантной и противовоспалительной активностью на интенсивность ПОЛ.

Aim. To study levels of conjugated dienes (CD), primary products of lipid peroxidation, and malondialdehyde (MDA), a secondary product of lipid peroxidation, in peritoneal exudate and serum of rats and mice with exudative inflammation. Materials and methods. CD and MDA concentrations were measured spectrophotometrically in peritoneal exudate from mice with glycogen-induced peritonitis and rats with acetic acid-induced peritonitis and in serum of rats with acetic-induced peritonitis and carrageenan-induced paw edema. Results. MDA concentrations were increased in the peritoneal exudate of rats with acetic acid-induced peritonitis and mice with glycogen-induced peritonitis as compared to intact animals. Both MDA and CD concentrations were increased in the serum of rats with acetic acid-induced peritonitis at 3 hours of the inflammation induction. However, only MDA levels significantly increased in rats with carrageenan-induced paw edema at 5 hours of the phlogogen injection, whereas CD levels did not differ from intact animals. Conclusion. These results might be useful for further investigation of changes in primary and secondary products of lipid peroxidation in experimental inflammatory conditions to characterize their pathogenesis and to study effects of antioxidative and anti-inflammatory compounds on concentrations of lipid peroxidation products.
Diagnostic informativity of hemodynamic identification of circulatory syndromes in heart failure
Authors: Белкания Г.С. , Диленян Л.Р. , Рыжаков Д.И. , Пухальская Л.Г.
Published: December 2020

Показана диагностическая информативность антропофизиологического и системного подхода в гемодинамической идентификации циркуляторных синдромов сердечной недостаточности (СН). Методы. Антропофизиологическая диагностика сердечно-сосудистой системы (ССС) осуществляли с использованием созданного нами аппаратно-программного комплекса на основе реографии. Результаты. По использованному алгоритму диагностики идентифицируются базовые циркуляторные синдромы СН по артериальной (перфузионной) и венозной недостаточности по малому и большому кругу кровообращения, а также возрастные, половые и позные особенности их проявления. Заключение. Показано, что по всем возрастным группам превалируют перфузионные формы СН. При этом проявления циркуляторных синдромов СН значительно выше в положении тела стоя.

The aim of the study was to demonstrate diagnostic informativity of the anthropophysiological and systemic approach to hemodynamic identification of circulatory syndromes in heart failure (HF). Methods. Anthropophysiological diagnostics of the cardiovascular system (CVS) was performed using a hardware-software complex created by us on the basis of rheography. Results. Based on the used diagnostic algorithm, essential circulatory HF syndromes were identified by arterial (perfusion) and venous insufficiency in the systemic and pulmonary circulation, age, sex, and postural features of the HF syndrome manifestations. Conclusion. Perfusion forms of HF prevailed in all age groups. Furthermore, manifestations of circulatory HF syndromes were much more pronounced in the standing body position.
Cell cultures of human malignant tumors in development of new anticancer therapies
Authors: Чернов А.Н. , Баранцевич Е.П. , Галимова Э.С. , Галагудза М.М.
Published: December 2020

Современный эффективный скрининг новых противоопухолевых химиопрепаратов и биологических препаратов на доклиническом этапе невозможен без применения моделей культур опухолевых клеток. К таким моделям относят первичные культуры клеток и клеточные линии опухолей человека, культивируемые в двумерной (2D) и трехмерной (3D) системах. В обзоре обсуждаются различные аспекты применения моделей клеточных культур неоплазий человека, их актуальность в исследованиях противоопухолевой эффективности препаратов.

Current effective preclinical screening of new anticancer chemotherapies and biological medicines requires cancer cell culture models. Such models include primary cell cultures and human tumor cell lines cultured in two-dimensional (2D) and three-dimensional (3D) systems. This review discussed different aspects of using human tumor cell culture models and their relevance for studying efficacy of antitumor drugs.
General approaches to anthropophysiological characterization of age-related changes in human circulation
Authors: Белкания Г.С. , Диленян Л.Р. , Багрий А.С. , Рыжаков Д.И. , Пухальская Л.Г.
Published: December 2020

В аналитическом обзоре показана динамика гидростатического (гравитационного) фактора кровообращения в соответствии с основными этапами адаптации в процессе развития и жизнедеятельности человека в условиях прямохождения (антропогенетическая модель постнатального онтогенеза). С этой динамикой сопоставляются имеющиеся данные по ростовому процессу массы сердца и минутного объема кровообращения (МОК). Демонстрируются принципиальные отличия возрастной динамики МОК по положению лежа и стоя. Особенно чётко эти отличия определяются по гидростатическому индексу сердца, который рассчитывается по отношению МОК (мл) на высоту гидростатического столба крови (см). Антропогенетическая модель постнатального онтогенеза обосновывает тип динамической организации сердечно-сосудистой системы (ССС) по соотношению МОК стоя/лежа (в %), представленной: гипокинетическим состоянием c уменьшением МОК в положении стоя по сравнению с положением лежа; эукинетическим (без изменений МОК); и гиперкинетическим состоянием с увеличением МОК в положении стоя. Показано, что основной направленностью возрастной динамики является перманентное уменьшение представленности гипокинетического состояния и возрастание представленности гиперкинетического состояния.

This analytical review analyzes the dynamics of the hydrostatic (gravitational) factor of circulation in accordance with major stages of adaptation in the process of human development and vital activity under the conditions of bipedal locomotion (anthropogenetic model of postnatal ontogenesis). This dynamics is compared with available data on growth-related changes in heart mass and cardiac output (CO). Fundamental differences are demonstrated for age-related CO changes in prone and upright positions. These differences particularly clearly evident from the hydrostatic heart index calculated as a ratio of MVB (ml) to the height of hydrostatic blood column (cm). The anthropogenetic model of postnatal ontogenesis determines the type of dynamic organization of the cardiovascular system (CVS) by the ratio of upright/prone CO (%) as the hypokinetic state with decreased upright CO compared to prone CO, eukinetic state (unchanged CO), and hyperkinetic state with increased upright CO. The major trend in age dynamics is a permanent decrease in the hypokinetic state and an increase in the hyperkinetic state.
The size of extracellular vesicles secreted by different types of stem cells
Authors: Алчинова И.Б. , Полякова М.В. , Сабурина И.Н. , Карганов М.Ю.
Published: December 2020

Механизм терапевтического действия мультипотентных мезенхимных стволовых клеток (ММСК) на облученный организм в последнее время вызывает повышенный интерес исследователей. В качестве активного участника паракринного механизма реализации этого эффекта предлагают рассматривать внеклеточные везикулы, секретируемые практически всеми клетками живого организма. Цель работы: выделить и охарактеризовать внеклеточные везикулы, продуцируемые стволовыми клетками различной природы. Материалы и методы. Суспензии внеклеточных везикул, выделенных по модифицированному протоколу дифференциального центрифугирования из культуральных жидкостей от культур ММСК костного мозга человека 2-го пассажа и ММСК жировой ткани крысы 4-го пассажа, были проанализированы методом просвечивающей электронной микроскопии и методом анализа траекторий наночастиц. Результаты. Исследование показало наличие в обоих образцах микрочастиц размерами до и около 100 нм, однако процентное содержание частиц разных размеров в суспензии различалось для двух анализируемых типов клеток. Заключение. Полученные результаты могут свидетельствовать о специфике секреции, обусловленной клеточным типом.

A mechanism of the therapeutic effect of multipotent mesenchymal stem cells (MMSC) on irradiated body has recently arisen much interest of researchers. Extracellular vesicles (EVs) secreted by almost all cells of a living organism were suggested to actively contribute to the paracrine mechanism of this effect. The aim of the study was isolation and characterization of extracellular vesicles produced by various types of stem cells. Materials and methods. Suspensions of EVs were isolated from culture media of passage 2 human bone marrow-derived MMSC and passage 4 rat adipose tissue-derived MMSC using a modified protocol of differential centrifugation and then studied using transmission electron microscopy and nanoparticle tracking analysis. Results. The study showed the presence of microparticles with a size of >100 nm in the examined samples. However, the percent content of particles with different sizes in the suspension was different in two analyzed types of cell culture. Conclusion. The study results might reflect a specificity of secretion determined by the cell type.
Controlo e infinitivos flexionados em português L2
Authors: Fiéis Alexandra , Madeira Ana
Published: December 2020

It is known that knowledge of the interpretative properties of the standard inflected infinitive develops late both in L1 and L2 acquisition, and that subject control with most verbs is acquired early in L1. In this study we focus on a context which has not been addressed so far, and investigate how the interpretation of the null subjects of inflected infinitival complements of subject control verbs develops in the interlanguage of Englishand Spanish-speaking learners of L2 European Portuguese. We applied a selection task to intermediate and advanced learners in order to understand whether they differentiate between inflected and uninflected infinitives in these contexts and whether they assign control properties to the inflected infinitive. Our findings show that, although learners accept the occurrence of inflected infinitives with subject control verbs and assign a controlled reading to the infinitival null subject, knowledge of some of the properties of these constructions is delayed, namely, which verbs allow inflected infinitives and what are the interpretative properties of inflected infinitival subjects under different control verbs.
Estruturas paratáticas de que-conetivo em frases não-argumentais
Authors: Matos Gabriela , Rodrigues Patrícia
Published: December 2020

In European and Brazilian Portuguese gramatical tradition, non-argumental clauses introduced by que ‘that’ have been classified as causal explicative clauses. However, differently from the other explicative conetives, que may head non-argumental clauses displaying other meanings, such as result/consequence or simultaneous temporal readings. We take these different readings as resulting from the context, and we assume that the main semantic import of que is acting as a sentence connector marker. Yet, despite the differences in the interpretation of queclauses, from a syntactic point of view, these clauses behave alike. We argue that they are parenthetical sentences, extending previous work on European Portuguese.
Avaliação de Narrativas Orais em Crianças Falantes de Português Europeu (PE): Um Teste Piloto
Authors: Vaz Stéphanie , Lobo Maria , Lousada Marisa
Published: December 2020

Although there are several referenced and validated international instruments for evaluating oral narratives,which can be used in populations with language disorders to help the diagnosis and to plan intervention, there is yet no such instrument for EP. In this work, we present a first version of an instrument for evaluating oral narrative productions for EP-speaking children, including the methodology used in the construction of the instrument, its structure, criteria for the analysis of productions, and procedures used in the validation of the instrument. In a second moment, we report the first results from a pilot study that indicate that there may be differences between tasks (telling or retelling), between types of stories and between age groups in what concerns the analysis of macrostructural parameters.
Erosão linguística na aquisição bilingue português – umbundu: evidências no domínio da formação do plural
Authors: Calossa Bernardino Valente , Flores Cristina
Published: December 2020

The present study assesses the linguistic development of Portuguese-Umbundu bilingual speakers in Angola by focusing on the production of plural forms. In particular, we analyse if changes in the degree of language exposure, after entering into the school, favor the occurrence of attrition in Umbundu, the home language, in parallel to the development of Portuguese, the dominant school language. A group of 28 bilingual students (14 3rd grade primary school students and 14 teenagers attending the 9th grade) and 28 age-matched monolingual speakers of Portuguese participated in a picture-based oral production elicitation task. The results show that the 3rd grade students have more difficulties in producing the plural in Portuguese, especially in situations where they have to apply particular rules (e.g. replace -ão with -ões), compared to Umbundu, the language that forms the plural by changing nominal classes. However, older students (9th graders) show more difficulties in forming the plural in Umbundu. Although this is not a longitudinal study, the data suggest that the change in the degree of language exposure, as a consequence of schooling in Portuguese, may have contributed to this inversion, which leads us to infer that the family language is vulnerable to attrition effects.
SP3-FAIMS Chemoproteomics for High Coverage Profiling of the Human Cysteinome
Authors: Yan Tianyang , Desai Heta , Boatner Lisa , Yen Stephanie , Cao Jian , Palafox Maria , Jami-Alahmadi Yasaman , Backus Keriann
Published: December 2020

We report a new cysteine chemoproteomic method, termed SP3-FAIMS chemoproteomics, which enables rapid and high coverage analysis of the human cysteinome. By combining enhanced cysteine biotinylation with SP3 sample decontamination and FAIMS online fraction, we identified in aggregate 34,225 unique cysteines found on 7,243 proteins. Showcasing the versatility of our method, integration with the isoTOP-ABPP workflow enabled the high throughput discovery of cysteines labelled by electrophilic compounds.
Large Peptide Permeation Through a Membrane Channel: Understanding Protamine Translocation Through CymA from Klebsiella Oxytoca
Authors: Pangeni Sushil , Prajapati Jigneshkumar Dahyabhai , Bafna Jayesh Arun , Mohamed Nilam , Nau Werner M. , Kleinekathöfer Ulrich , Winterhalter Mathias
Published: December 2020

Quantifying the passage of the large peptide protamine (Ptm) across CymA, a passive channel for cyclodextrin uptake, is in the focus of this study. Using a reporter-pair based fluorescence membrane assay we detected the entry of Ptm into liposomes containing CymA. The kinetics of the Ptm entry was independent of its concentration suggesting that the permeation across CymA is the rate-limiting factor. Furthermore, we reconstituted single CymA channels into planar lipid bilayers and recorded the ion current fluctuations in the presence of Ptm. To this end, we were able to resolve the voltage-dependent entry of single Ptm peptide molecules into the channel. Extrapolation to zero voltage revealed about 1-2 events per second and long dwell times, in agreement with the liposome study. Applied-field and steered molecular dynamics simulations provided an atomistic view on the permeation. It can be concluded that a concentration gradient of 1 M Ptm leads to a translocation rate of about 1 molecule per second and per channel.
IDENTITAS KELOKALAN SEBAGAI REPRESENTASI DALAM ALKITAB BAHASA MELAYU KUPANG Indentity of The Locals as Represented in The Language of The Kupang-Malay Bible
Authors: Thans Peggy Magdalena Jona
Published: December 2020

AbstrakArtikel ini memaparkan identitas komunitas penutur bahasa (kreol) yang direpresentasikan di dalam Alkitab terjemahan Melayu-Kupang. Penutur bahasa, secara implisit, cenderung menunjukkan kepercayaanya, nilai yang dianut, serta kebutuhan melalui wacana dalam komunikasi. Hal tersebut terwujud melalui interaksi terdahulu hingga saat kini. ‘Index of importance’ atau kategori kepentingan dari suatu komunitas dirasakan oleh penuturnya melalui ciri linguistik kebahasaan karena bahasa membentuk tatanan sosial dan interaksi suatu relasi. Setiap penutur bahasa terasosiasi dengan penanda leksikal tertentu dan penanda tersebut berganti seiring perubahan konteks komunikasi yang terjadi. Identitas lokal yang digambarkan Alkitab Terjemahan Bahasa (kreol) Kupang berdasarkan prinsip penerjemahan kebermaknaan, dan mengintegrasikan budaya dan tradisi komunitas penutur kreol MelayuKupang. Inti analisis mencakup indeks perubahan dalam status sosial; nama baru, panggilan sapaan dalam relasi horisontal dan vertikal, kesopanan bahasa, kata leksikal, dan konteks situasi. Disimpulkan bahwa komunitas penutur kreol Melayu Kupang yang digambarkan dalam Alkitab terjemahan tersebut belum seutuhnya tercermin berdasarkan keadaan dan tradisi sesungguhnya: kategori rujukan dalam interaksi sosial dengan penggunaan ‘register’ yang berbeda; pilihan diksi untuk wacana bervariasi; tingkat kesopanan; relasi vertikal dan horisontal. Revisi pada terjemahan Alkitab KM sangat diperlukan karena merefleksikan gambaran komunitas Melayu Kupang kepada publik. Kata-kata kunci: konstruksi interaksi, status sosial, identitas setempat AbstractThis present paper discusses the local identity represented in the Kupang-Malay creole translated Bible. Language speakers implicitly reflect their beliefs, values, and needs through their language and discourse during communication. They were shared through past and present interaction. The speakers index of importance or category of interest is experienced by the speakers and manifested into linguistics features. Within this social construct, each speaker is associated with specific lexical markers in different contexts. This paper discusses how Kupang-Malay reflects local identity. The analysis covers the index of change, terms of address in the vertical and horizontal direction. The primary data is Kupang Malay Bible. The results of the analysis show that overall the Kupang-Malay community has not represented well in the Bible: in terms of the category of reference they commonly use, registers, level of politeness in language usage across ages, vertical and horizontal relationships. A room for improvement on the Bible translation is needed to represent the Kupang-Malay community better.Keywords: interactional construct, social status, local identity
NILAI-NILAI KEAGAMAAN DALAM PARIKAN GONDANGKELI TARI TOPENG LENGGER WONOSOBO Religius Value of Parikan Gondangkeli in Wonosobo's Lengger Mask Dace Performance
Authors: Fikri Mochammad
Published: December 2020

AbstrakParikan Gondangkeli merupakan salah satu sastra lisan yang sarat dengan nilai-nilai keagamaan. Parikan sangat menarik untuk dikaji sebab sepengetahuan peneliti kajian terhadap parikan ini masih sangat sedikit. Penelitian ini bertujuan untuk mendeskripsikan nilai-nilai keagamaan yang terdapat dalam Parikan Gondangkeli Tari Topeng Lengger Wonosobo. Pendekatan yang digunakan untuk meneliti parikan tersebut adalah pendekatan pragmatik, sebuah pendekatan dalam penelitian sastra yang akan menguak kebermanfaatan suatu karya sastra bagi masyarakat. Teori yang digunakan sebagai dasar telaah terhadap parikan tersebut adalah teori dimensi religiositas yang dikemukakan oleh Glock dan Stark. Data penelitian ini adalah sebuah parikan yang berjudul Parikan Gondangkeli. Parikan tersebut merupakan salah satu parikan yang digunakan untuk mengiringi seni pertunjukan Tari Topeng Lengger Wonosobo. Data didapatkan dengan menggunakan metode telaah pustaka dan metode observasi atau metode pengamatan langsung ke lapangan. Hasil penelitian menunjukkan bahwa dimensi religiositas yang terepresentasi dalam Parikan Gondangkeli Tari Topeng Lengger Wonosobo adalah 1) dimensi religiositas keyakinan atau ideologi; 2) dimensi ritualistik; 3) dimensi ihsan; 4) dimensi pengetahuan atau pengalaman; dan 5) dimensi pengamalan dan konsekuensi.Kata Kunci: nilai-nilai, keagamaan, parikan GondangkeliAbstractParikan Gondangkeli is an oral literature in the tradition of both poetry and dance set within rich religious values. The parikan is very interesting to be investigated due to the lack of study of the area. This study aims to describe the religious values in the Gondangkeli Parikans performed in Lengger Wonosobo Mask Dance. The approach applied in this research is a pragmatic approach, an approach in literary research that reveals the usefulness of a literary work for society. The theory applied as the basis for the analysis is the theory of dimensions of religiosity put forward by Glock and Stark (1972). The data of this research cover any lines of parikan entitled Parikan Gondangkeli. The parikan is one of the various parikans sang as a accompaniment in the performing arts of the Wonosobo Lengger Mask Dance. A literature review and observation methods or direct observation method are applied in the data collecting procedure. The findings of this research show that the dimensions of religiosity represented in the Parikan Gondangkeli cover 1) religious belief; 2) ritualistic; 3) external practise, 4) religious exclusivity (knowledge or experience); and 5) personal practice and consequence dimensions.Keywords: values, religiosity, parikan of Gondangkeli
Tingkat Kesantunan Nonverbal dalam Tuturan Verbal antara Penjual dan Pembeli di Pasar Beringharjo Yogyakarta: Kajian Etnopragmatik
Authors: Pranowo NFN
Published: December 2020

The levels of nonverbal politeness in verbal speech acts is an ethnopragmatic study. The research design was descriptive qualitative with ethnopragmatic basic theory. Ethnographic theory is basically a theory commonly used to study the culture of an ethnicity. In further developments, ethnography can be used to study cultural acts, including speech acts in language. Cultural acts in language cannot be separated from the context of language usage. Therefore, this study also involves pragmatic theory. Cultural speech acts can occur in various environments of speech events. This research will examine speech acts that occur in the cultural environment of the Beringharjo market in Yogyakarta when traders and buyers transact with each other. The research objectives are (a) to describe the form of nonverbal politeness used by buyers and sellers in the Beringharjo market, and (b) to describe the factors that influence the level of nonverbal politeness in verbal speech by the seller-buyer. AbstrakTingkat kesantunan nonverbal dalam tindak tutur verbal merupakan kajian etnopragmatik. Desain penelitian adalah deskripsitif kualitatif dengan teori dasar etnopragmatik. Teori etnografi pada dasarnya merupakan teori yang biasa digunakan untuk mengkaji kebudayaan suatu etnis. Dalam perkembangan selanjutnya, etnografi dapat dipakai untuk mengkaji tindak budaya, termasuk tindak tutur berbahasa. Tindak budaya dalam berbahasa tidak dapat dilepaskan dengan konteks pemakaian bahasa. Oleh karena itu, kajian ini juga melibatkan teori pragmatik. Tindak tutur budaya dapat terjadi di berbagai lingkungan peristiwa tutur. Penelitian ini akan mengkaji tindak tutur yang terjadi di lingkungan budaya Pasar Beringharjo Yogyakarta ketika pedagang-pembeli saling bertransaksi. Tujuan penelitiannya adalah (a) mendeskripsikan wujud kesantunan nonverbal yang digunakan oleh penjual-pembeli dan antarpedagang di Pasar Beringharjo, dan (b) mendeskripsikan faktor yang memengaruhi tingkat kesantunan nonverbal dalam tuturan verbal oleh penjual-pembeli.
DIKSI BAHASA INDONESIA DALAM SURAT DINAS, LAPORAN, DAN PAPAN NAMA RUANG PADA BADAN PUBLIK DI DAERAH ISTIMEWA YOGYAKARTA
Authors: Sumadi NFN
Published: December 2020

This research discusses diction in the Indonesian language in official letters, reports, and names of rooms in official public bodies in Daerah Istimewa Yogyakarta. The theory used in this research is a structural linguistic theory, particularly dealing with the element and structure of the Indonesian language in official letters, reports, and room board names. The method used is prescriptive or normative which is combined with the theory of analysis on language speaking. It means that this research has identified a thoroughly lingual unit that is inappropriate to language norms and it was substituted by the correct language unit. The research data are official letters, reports, and name boards which show wrong dictions on public officials in Daerah Istimewa Yogyakarta. The research source of data are official letters text, reports, and name boards which show in rumah sakit umum daerah, kecamatan kota, dinas pendidikan, badan kepegawaian daerah, and sekretariat daerah in Sleman, Kulon Progo, Bantul, Gunungkidul regencies, and Kota Yogyakarta in 2013—2019. The wrong dictions in Indonesia language in official letters, reports, and name board come from foreign and local words that have Indonesian meaning or words which is inappropriate to Indonesian grammar.Penelitian ini membahas diksi bahasa Indonesia dalam surat dinas, laporan, dan papan nama ruang pada badan publik di Daerah Istimewa Yogyakarta. Teori yang digunakan dalam penelitian ini ialah teori linguistik struktural, khususnya yang berkaitan dengan unsur dan struktur bahasa Indonesia yang digunakan dalam surat dinas, laporan, dan papan nama ruang. Metode yang digunakan ialah metode preskriptif atau normatif yang dipadu dengan teori analisis kesalahan berbahasa. Dalam penelitian ini satuan lingual  yang  tidak sesuai dengan norma kebahasaan diidentifikasi secara terperinci dan diganti dengan satuan lingual yang benar. Data penelitian ini ialah bahasa surat dinas, laporan, dan papan nama ruang dari badan publik di Daerah Istimewa Yogyakarta yang mengandung kesalahan diksi. Sumber data penelitian ini ialah teks surat dinas, laporan, dan papan nama ruang pada rumah sakit umum daerah, kecamatan kota, dinas pendidikan, badan kepegawaian daerah, dan sekretariat daerah di Kabupaten Sleman, Kulon Progo, Bantul, Gunungkidul, dan Kota Yogyakarta yang terbit pada tahun 2013—2019. Kesalahan diksi bahasa Indonesia dalam surat dinas, laporan, dan papan nama ruang  disebabkan oleh penggunaan kata asing dan kata daerah yang padanannya sudah ada dalam bahasa Indonesia atau penggunaan kata yang tidak sesuai dengan kaidah tata bentuk kata dalam bahasa Indonesia.
KESESUAIAN BAHAN BACAAN LITERASI EMERGEN DENGAN PEMBACA SASARAN
Authors: Sidik Umar
Published: December 2020

The problem of this study departs from a lot of reading material that is suspected to be incompatible with the target audience (emergent literacy). Readings that are labeled for pre-reading, but the content does not suit the target audience. In this connection, the purpose of this study is to find the incongruity of reading material with the target audience for emergency literacy based on reading gap standards. This study refers to the standardization of non-text reading material set by the Ministry of Education and Culture (2018). The data source is determined by purposive sampling technique. Data collection is done by listening, reading, and note / record techniques. The analysis is carried out by means of interpreting and interpreting the data to obtain inference in accordance with the research objectives. The results showed that the literacy reading materials emegen circulating in the majority of the community did not match (match) with the target reader (emergency literacy). Many readings put forward or give priority to the message content of the narrative story. Pictures / illustrations are more oriented to support the narrative of the story. There is almost no process of introducing the beginning of writing, from the alphabetical or phonological as early as learning literacy literacy.Permasalahan penelitian ini berangkat dari banyak bahan bacaan yang ditengarai tidak sesuai dengan pembaca sasaran (literasi emergen). Bacaan yang dilabeli untuk pramembaca, tetapi isinya tidak sesuai dengan pembaca sasarannya. Sehubungan dengan itu, tujuan penelitian ini ialah untuk menemukan ketidaksesuaian bahan bacaan dengan pembaca sasaran literasi emergen berdasarkan standar penjenjangan bacaan. Penelitian ini mengacu pada standar penjenjangan bahan bacaan nonteks yang ditetapkan oleh Kemendikbud (2018). Sumber data ditentukan dengan teknik purposive sampling. Pengumpulan data dilakukan dengan teknik simak, baca, dan catat/rekam. Analisis dilakukan dengan cara penginterpretasian dan pemaknaan data untuk memperoleh inferensi sesuai dengan tujuan penelitian. Hasil penelitian menunjukkan bahwa bahan bacaan literasi emergen yang beredar di masyarakat mayoritas tidak sesuai (matching) dengan pembaca sasarannya (literasi emergen). Banyak bacaan yang mengedepankan atau mengutamakan pesan isi dari narasi cerita. Gambar/ilustrasi lebih diorientasikan untuk mendukung narasi cerita. Hampir tidak ada proses pengenalan awal terhadap tulisan, dari alfabetik atau fonologis sebagi awal membelajarkan literasi baca-tulis.
Pythagorean fuzzy α - continuity
Authors: J Joseline Charisma , D Ajay
Published: December 2020

In this research article, we present the new concept of Pythagorean fuzzy α− continuous function between Pythagorean fuzzy topological spaces and establish their corresponding characterizations.
A study on some infinite server queues in discrete-time
Authors: Hussainy Syed Tahir , D Lokesh
Published: December 2020

This work analysis some discrete-time queueing mechanisms with infinitely many servers.By using a shot noise process, general results on the system size in discrete-time are given both in transient state and in steady state. For this we use the classical differentiation formula of F´a di Bruno. First two moments of the system size and distribution of the busy period of the system are also computed.
Inter-relações entre os gêneros feminino e masculino: representações sociais que perpassam o âmbito escolar
Authors: Gonçalves Josiane Peres , Ferreira Marta Claudiane
Published: December 2020

O presente estudo tem o propósito de identificar as representações sociais da comunidade escolar do Estado de Mato Grosso do Sul, sobre as relações de gênero feminino e masculino. A pesquisa de natureza qualitativa foi realizada por meio de um roteiro de entrevistas semiestruturadas, que foram gravadas em escolas municipais de Campo Grande, Corumbá, Itaquiraí e Tacuru, com um total de 13 participantes sendo: 5 familiares, 1 criança, 3 professores e 4 gestores escolares. Os resultados sugerem que as representações de gênero feminino e masculino são norteadas pelos valores e experiências adquiridas no contexto social em que os participantes estão inseridos. Essas são reproduzidas e repassadas às próximas gerações, sem ao menos analisar se sua maneira de agir e pensar estão corretas. Contudo, tais representações podem ser descontruídas mediante novos olhares de formadores de opinião, como os profissionais da educação.
Pedagogia da Alternância: letramentos na Educação do Campo
Authors: Silva Carlos Antônio Andrade , Neves Miranilde Oliveira
Published: December 2020

Este artigo objetiva investigar de que forma ocorre a aprendizagem dos letramentos nas aulas de linguagens, quando mediados pela alternância pedagógica. É resultado de um projeto de pesquisa desenvolvido com turmas do PROEJA de um Instituto Federal de Educação do Norte do Brasil. Optou-se pela abordagem qualitativa, pois esta se preocupa com aspectos da realidade produzida pela interação entre o tempo escola e o tempo comunidade: pedagogia da alternância. Nesse contexto, o levantamento de dados ocorreu por meio da revisão bibliográfica de referências em Educação do Campo: Camilo (2008), Arroyo (2004); dos Letramentos: Soares (2006), (2009) Marcuschi (2001), Rojo; Moura (2012); e da Pedagogia da Alternância: Brambati (2014), Gimonet (1999), e por meio de questionários semiestruturados destinados aos professores de linguagens, coordenador e alunos do PROEJA. No que se refere às práticas de letramentos, confirmou-se que estas só ocorrem de forma significativa, quando os professores compreendem e aplicam a pedagogia da alternância de forma que os gêneros textuais do cotidiano, produzidos no tempo comunidade, dialoguem com as práticas pedagógicas desenvolvidas no tempo escola – questão comprovada nas respostas dos participantes da pesquisa.
Introducing a Novel Undergraduate Research Education Initiative: The URNCST Journal Mentored Paper
Authors: Fakuade Ayomide , Nagy Stephanie , Pavithran Neethu , Siddique Saameh , Ng Jeremy Y.
Published: December 2020

The Undergraduate Research in Natural and Clinical Science and Technology (URNCST) Journal Mentored Paper initiative is a unique undergraduate research education opportunity open to undergraduate and professional-undergraduate degree students internationally. Participants are invited to submit an abstract on a selected topic and be paired with a graduate student mentor with an interest and expertise in the research area. Over a three-month period, the mentor and mentee(s) work together to turn the unpolished abstract into a full-length manuscript of publishable quality. In this short editorial, we provide an overview of how our editorial team successfully conceptualized, developed, and established this initiative. Undergraduate students interested in submitting an abstract to the next URNCST Journal Mentored Paper round should visit: https://www.urncst.com/index.php/urncst/mentored_paper. Graduate students interested in serving as a mentor should visit https://www.urncst.com/index.php/urncst/about/#_Toc487899585to apply to the URNCST Journal.