bluuebunny/crossref_metadata_2025 huggingface dataset:
A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties
Authors: Zhang Yuqian , Murakami Kazutoshi , Borra Vishnupriya J. , Ozen Mehmet Ozgun , Demirci Utkan , Nakamura Takahisa , Esfandiari Leyla
Published: February 2022

Extracellular vesicles (EVs) have gained considerable attention as vital circulating biomarkers since their structure and composition resemble the originating cells. The investigation of EVs’ biochemical and biophysical properties is of great importance to map them to their parental cells and to better understand their functionalities. In this study, a novel frequency-dependent impedance measurement system has been developed to characterize EVs based on their unique dielectric properties. The system is composed of an insulator-based dielectrophoretic (iDEP) device to entrap and immobilize a cluster of vesicles followed by utilizing electrical impedance spectroscopy (EIS) to measure their impedance at a wide frequency spectrum, aiming to analyze both their membrane and cytosolic charge-dependent contents. The EIS was initially utilized to detect nano-size vesicles with different biochemical compositions, including liposomes synthesized with different lipid compositions, as well as EVs and lipoproteins with similar biophysical properties but dissimilar biochemical properties. Moreover, EVs derived from the same parental cells but treated with different culture conditions were characterized to investigate the correlation of impedance changes with biochemical properties and functionality in terms of pro-inflammatory responses. The system also showed the ability to discriminate between EVs derived from different cellular origins as well as among size-sorted EVs harbored from the same cellular origin. This proof-of-concept approach is the first step towards utilizing EIS as a label-free, non-invasive, and rapid sensor for detection and characterization of pathogenic EVs and other nanovesicles in the future.
Accuracy Assessment of the GlucoMen® Day CGM System in Individuals with Type 1 Diabetes: A Pilot Study
Authors: Hochfellner Daniel A. , Simic Amra , Taucher Marlene T. , Sailer Lea S. , Kopanz Julia , Pöttler Tina , Mader Julia K.
Published: February 2022

The aim of this study was to evaluate the accuracy and usability of a novel continuous glucose monitoring (CGM) system designed for needle-free insertion and reduced environmental impact. We assessed the sensor performance of two GlucoMen® Day CGM systems worn simultaneously by eight participants with type 1 diabetes. Self-monitoring of blood glucose (SMBG) was performed regularly over 14 days at home. Participants underwent two standardized, 5-h meal challenges at the research center with frequent plasma glucose (PG) measurements using a laboratory reference (YSI) instrument. When comparing CGM to PG, the overall mean absolute relative difference (MARD) was 9.7 [2.6–14.6]%. The overall MARD for CGM vs. SMBG was 13.1 [3.5–18.6]%. The consensus error grid (CEG) analysis showed 98% of both CGM/PG and CGM/SMBG pairs in the clinically acceptable zones A and B. The analysis confirmed that GlucoMen® Day CGM meets the clinical requirements for state-of-the-art CGM. In addition, the needle-free insertion technology is well tolerated by users and reduces medical waste compared to conventional CGM systems.
Reconfiguring Vertical Urbanism: The Example of Tall Buildings and Transit-Oriented Development (TB-TOD) in Hong Kong
Authors: Al-Kodmany Kheir , Xue Qiuli (Charlie) , Sun Cong
Published: February 2022

As the world moves toward becoming 70% urbanized by 2050, urban density becomes imperative in decisions on the urban environment and living quality. This paper builds on the polycentric and “rail village” development model that applies a “concentrated” density in strategic locations to create hyper-connected, socially vibrant hubs. Notably, it proposes the Tall Buildings and Transit-Oriented Development (TB-TOD) model, a type of development that refers to vertical mixed-use developments centered on mass transit nodes, as a sustainable option for large cities going forward. The key factors that promote the TB-TOD model are the efficient use of costly land, increased population density, boosted ridership, better regional connectivity, more sensible suburban growth, and improved placemaking. The paper examines the relationship between high-rise buildings and TOD in Hong Kong. The paper identifies and compares four types of TOD model, including “plug-in” TOD in the old city; city-edge TOD; “one building” TOD; and suburban TOD in the new area. Overall, this study documents urban design prototypes applicable to cities facing the challenges of high urban density and an excessive population.
Evolution of Electronic Procurement: Contemporary Review of Adoption and Implementation Strategies
Authors: Chan Albert P. C. , Owusu Emmanuel Kingsford
Published: February 2022

Since the advent of the first form of electronic procurement (EP), the procurement process (i.e., the stages and underlying activities) has evolved to transform the world of commerce significantly. In the construction industry, EP has revolutionized the operational schema of traditional procurement and has paved the way for the development, advancement, and application of more intelligent tools for handling and executing procurement activities of infrastructure-related works. Although the development or adoption of EP-related tools has not been encouraging due to the industry’s conservative nature, in this study, we review the evolution and developments of EP to date. It explores the adoption and implementation strategies of extant and projected EP developments and develops a conceptual framework to explicate the developmental transitions of EP. While there are many studies on EP, there is still a long way to transition into a more intelligent execution of EP-related activities. A total of 64 EP-related manuscripts and other valuable studies were examined to realize the stipulated objective as well as identify the thematic constructs developed under the subject matter. The explorations conducted in this study are intended to contribute to the understanding of the extant discussion on both EP and smart procurement (SP), their adoption strategies, implementation, and applications in infrastructure projects. It elucidates the significant constructs that define and underpin the theoretical implications of EP and SP adoption and implementation. Lastly, the framework highlights the projected developments and transition phases of EP to intelligent procurement (IP).
Chloride Channels and Transporters: Roles beyond Classical Cellular Homeostatic pH or Ion Balance in Cancers
Authors: Kim Hyeong Jae , Lee Peter Chang-Whan , Hong Jeong Hee
Published: February 2022

The canonical roles of chloride channels and chloride-associated transporters have been physiologically determined; these roles include the maintenance of membrane potential, pH balance, and volume regulation and subsequent cellular functions such as autophagy and cellular proliferative processes. However, chloride channels/transporters also play other roles, beyond these classical function, in cancerous tissues and under specific conditions. Here, we focused on the chloride channel-associated cancers and present recent advances in understanding the environments of various types of cancer caused by the participation of many chloride channel or transporters families and discuss the challenges and potential targets for cancer treatment. The modulation of chloride channels/transporters might promote new aspect of cancer treatment strategies.
LiKidMiRs: A ddPCR-Based Panel of 4 Circulating miRNAs for Detection of Renal Cell Carcinoma
Authors: Sequeira José Pedro , Constâncio Vera , Salta Sofia , Lobo João , Barros-Silva Daniela , Carvalho-Maia Carina , Rodrigues Jéssica , Braga Isaac , Henrique Rui , Jerónimo Carmen
Published: February 2022

Background: Decreased renal cell cancer-related mortality is an important societal goal, embodied by efforts to develop effective biomarkers enabling early detection and increasing the likelihood of curative treatment. Herein, we sought to develop a new biomarker for early and minimally invasive detection of renal cell carcinoma (RCC) based on a microRNA panel assessed by ddPCR. Methods: Plasma samples from patients with RCC (n = 124) or oncocytomas (n = 15), and 64 healthy donors, were selected. Hsa-miR-21-5p, hsa-miR-126-3p, hsa-miR-155-5p and hsa-miR-200b-3p levels were evaluated using a ddPCR protocol. Results: RCC patients disclosed significantly higher circulating levels of hsa-miR-155-5p compared to healthy donors, whereas the opposite was observed for hsa-miR-21-5p levels. Furthermore, hsa-miR-21-5p and hsa-miR-155-5p panels detected RCC with high sensitivity (82.66%) and accuracy (71.89%). The hsa-miR-126-3p/hsa-miR-200b-3p panel identified the most common RCC subtype (clear cell, ccRCC) with 74.78% sensitivity. Conclusion: Variable combinations of plasma miR levels assessed by ddPCR enable accurate detection of RCC in general, and of ccRCC. These findings, if confirmed in larger studies, provide evidence for a novel ancillary tool which might aid in early detection of RCC.
Regulation of B-Cell Receptor Signaling and Its Therapeutic Relevance in Aggressive B-Cell Lymphomas
Authors: Profitós-Pelejà Núria , Santos Juliana Carvalho , Marín-Niebla Ana , Roué Gaël , Ribeiro Marcelo Lima
Published: February 2022

The proliferation and survival signals emanating from the B-cell receptor (BCR) constitute a crucial aspect of mature lymphocyte’s life. Dysregulated BCR signaling is considered a potent contributor to tumor survival in different subtypes of B-cell non-Hodgkin lymphomas (B-NHLs). In the last decade, the emergence of BCR-associated kinases as rational therapeutic targets has led to the development and approval of several small molecule inhibitors targeting either Bruton’s tyrosine kinase (BTK), spleen tyrosine kinase (SYK), or phosphatidylinositol 3 kinase (PI3K), offering alternative treatment options to standard chemoimmunotherapy, and making some of these drugs valuable assets in the anti-lymphoma armamentarium. Despite their initial effectiveness, these precision medicine strategies are limited by primary resistance in aggressive B-cell lymphoma such as diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL), especially in the case of first generation BTK inhibitors. In these patients, BCR-targeting drugs often fail to produce durable responses, and nearly all cases eventually progress with a dismal outcome, due to secondary resistance. This review will discuss our current understanding of the role of antigen-dependent and antigen-independent BCR signaling in DLBCL and MCL and will cover both approved inhibitors and investigational molecules being evaluated in early preclinical studies. We will discuss how the mechanisms of action of these molecules, and their off/on-target effects can influence their effectiveness and lead to toxicity, and how our actual knowledge supports the development of more specific inhibitors and new, rationally based, combination therapies, for the management of MCL and DLBCL patients.
Intestinal Epithelial AMPK Deficiency Causes Delayed Colonic Epithelial Repair in DSS-Induced Colitis
Authors: Olivier Séverine , Diounou Hanna , Pochard Camille , Frechin Lisa , Durieu Emilie , Foretz Marc , Neunlist Michel , Rolli-Derkinderen Malvyne , Viollet Benoit
Published: February 2022

Dysfunctions in the intestinal barrier, associated with an altered paracellular pathway, are commonly observed in inflammatory bowel disease (IBD). The AMP-activated protein kinase (AMPK), principally known as a cellular energy sensor, has also been shown to play a key role in the stabilization and assembly of tight junctions. Here, we aimed to investigate the contribution of intestinal epithelial AMPK to the initiation, progression and resolution of acute colitis. We also tested the hypothesis that protection mediated by metformin administration on intestinal epithelium damage required AMPK activation. A dextran sodium sulfate (DSS)-induced colitis model was used to assess disease progression in WT and intestinal epithelial cell (IEC)-specific AMPK KO mice. Barrier integrity was analyzed by measuring paracellular permeability following dextran-4kDa gavage and pro-inflammatory cytokines and tight junction protein expression. The deletion of intestinal epithelial AMPK delayed intestinal injury repair after DSS exposure and was associated with a slower re-epithelization of the intestinal mucosa coupled with severe ulceration and inflammation, and altered barrier function. Following intestinal injury, IEC AMPK KO mice displayed a lower goblet cell counts with concomitant decreased Muc2 gene expression, unveiling an impaired restitution of goblet cells and contribution to wound healing process. Metformin administration during the recovery phase attenuated the severity of DSS-induced colitis through improvement in intestinal repair capacity in both WT and IEC AMPK KO mice. Taken together, these findings demonstrate a critical role for IEC-expressed AMPK in regulating mucosal repair and epithelial regenerative capacity following acute colonic injury. Our studies further underscore the therapeutic potential of metformin to support repair of the injured intestinal epithelium, but this effect is conferred independently of intestinal epithelial AMPK.
Cell-Cell Interaction-Mediated Signaling in the Testis Induces Reproductive Dysfunction—Lesson from the Toxicant/Pharmaceutical Models
Authors: Wang Lingling , Bu Tiao , Wu Xiaolong , Gao Sheng , Li Xinyao , De Jesus Angela Bryanne , Wong Chris K. C. , Chen Hao , Chung Nancy P. Y. , Sun Fei , Cheng C. Yan
Published: February 2022

Emerging evidence has shown that cell-cell interactions between testicular cells, in particular at the Sertoli cell-cell and Sertoli-germ cell interface, are crucial to support spermatogenesis. The unique ultrastructures that support cell-cell interactions in the testis are the basal ES (ectoplasmic specialization) and the apical ES. The basal ES is found between adjacent Sertoli cells near the basement membrane that also constitute the blood-testis barrier (BTB). The apical ES is restrictively expressed at the Sertoli-spermatid contact site in the apical (adluminal) compartment of the seminiferous epithelium. These ultrastructures are present in both rodent and human testes, but the majority of studies found in the literature were done in rodent testes. As such, our discussion herein, unless otherwise specified, is focused on studies in testes of adult rats. Studies have shown that the testicular cell-cell interactions crucial to support spermatogenesis are mediated through distinctive signaling proteins and pathways, most notably involving FAK, Akt1/2 and Cdc42 GTPase. Thus, manipulation of some of these signaling proteins, such as FAK, through the use of phosphomimetic mutants for overexpression in Sertoli cell epithelium in vitro or in the testis in vivo, making FAK either constitutively active or inactive, we can modify the outcome of spermatogenesis. For instance, using the toxicant-induced Sertoli cell or testis injury in rats as study models, we can either block or rescue toxicant-induced infertility through overexpression of p-FAK-Y397 or p-FAK-Y407 (and their mutants), including the use of specific activator(s) of the involved signaling proteins against pAkt1/2. These findings thus illustrate that a potential therapeutic approach can be developed to manage toxicant-induced male reproductive dysfunction. In this review, we critically evaluate these recent findings, highlighting the direction for future investigations by bringing the laboratory-based research through a translation path to clinical investigations.
Anti-microRNA-21 Therapy on Top of ACE Inhibition Delays Renal Failure in Alport Syndrome Mouse Models
Authors: Rubel Diana , Boulanger Joseph , Craciun Florin , Xu Ethan Y. , Zhang Yanqin , Phillips Lucy , Callahan Michelle , Weber William , Song Wenping , Ngai Nicholas , Bukanov Nikolay O. , Shi Xingyi , Hariri Ali , Husson Hervé , Ibraghimov-Beskrovnaya Oxana , Liu Shiguang , Gross Oliver
Published: February 2022

Col4a3−/− Alport mice serve as an animal model for renal fibrosis. MicroRNA-21 (miR-21) expression has been shown to be increased in the kidneys of Alport syndrome patients. Here, we investigated the nephroprotective effects of Lademirsen anti-miR-21 therapy. We used a fast-progressing Col4a3−/− mouse model with a 129/SvJ background and an intermediate-progressing F1 hybrid mouse model with a mixed genetic background, with angiotensin-converting enzyme inhibitor (ACEi) monotherapy in combination with anti-miR-21 therapy. In the fast-progressing model, the anti miR-21 and ACEi therapies showed an additive effect in the reduction in fibrosis, the decline of proteinuria, the preservation of kidney function and increased survival. In the intermediate-progressing F1 model, the anti-miR-21 and ACEi therapies individually improved kidney pathology. Both also improved kidney function and survival; however, the combination showed a significant additive effect, particularly for survival. RNA sequencing (RNA-seq) gene expression profiling revealed that the anti-miR-21 and ACEi therapies modulate several common pathways. However, anti-miR-21 was particularly effective at normalizing the expression profiles of the genes involved in renal tubulointerstitial injury pathways. In conclusion, significant additive effects were detected for the combination of anti-miR-21 and ACEi therapies on kidney function, pathology and survival in Alport mouse models, as well as a strong differential effect of anti-miR-21 on the renal expression of fibrotic factors. These results support the addition of anti-miR-21 to the current standard of care (ACEi) in ongoing clinical trials in patients with Alport syndrome.
The Evolution of Biomineralization through the Co-Option of Organic Scaffold Forming Networks
Authors: Ben-Tabou de-Leon Smadar
Published: February 2022

Biomineralization is the process in which organisms use minerals to generate hard structures like teeth, skeletons and shells. Biomineralization is proposed to have evolved independently in different phyla through the co-option of pre-existing developmental programs. Comparing the gene regulatory networks (GRNs) that drive biomineralization in different species could illuminate the molecular evolution of biomineralization. Skeletogenesis in the sea urchin embryo was extensively studied and the underlying GRN shows high conservation within echinoderms, larval and adult skeletogenesis. The organic scaffold in which the calcite skeletal elements form in echinoderms is a tubular compartment generated by the syncytial skeletogenic cells. This is strictly different than the organic cartilaginous scaffold that vertebrates mineralize with hydroxyapatite to make their bones. Here I compare the GRNs that drive biomineralization and tubulogenesis in echinoderms and in vertebrates. The GRN that drives skeletogenesis in the sea urchin embryo shows little similarity to the GRN that drives bone formation and high resemblance to the GRN that drives vertebrates’ vascular tubulogenesis. On the other hand, vertebrates’ bone-GRNs show high similarity to the GRNs that operate in the cells that generate the cartilage-like tissues of basal chordate and invertebrates that do not produce mineralized tissue. These comparisons suggest that biomineralization in deuterostomes evolved through the phylum specific co-option of GRNs that control distinct organic scaffolds to mineralization.
Anti-Aging Effects of a Serum Based on Coconut Oil Combined with Deer Antler Stem Cell Extract on a Mouse Model of Skin Aging
Authors: Pham Truc Le-Buu , Thi Thuy Truong , Nguyen Huyen Thi-Thuong , Lao Thuan Duc , Binh Nguyen Trong , Nguyen Quan Dang
Published: February 2022

Anti-aging is one of the top goals in the field of health care and aesthetics. Anti-aging cosmetics derived from nature are oriented to long-term development, bringing safety to users and being environmentally friendly. The aim of this study was to develop an anti-aging cosmetic formulation process based on coconut oil in combination with deer antler stem cell extract. The results show that the presence of deer antler stem cell extract added to the foundation made the serum product highly stable and helped improve skin aging significantly after 2 weeks of use. The skin site where the serum product was applied showed a smooth and elastic skin surface, with very few fine lines and shallow wrinkles. Serum reduced the number of wrinkles (48.09% compared to commercial serum (ME) and 60.31% compared to positive control (PC)), reduced skin recovery time (39.31% compared to ME and 67.1% of PC) after two weeks of use. After 2 weeks of use, collagen density increased 10.18% compared to ME and 63.76% compared to control. Epidermal thickness increased by 106.1% compared to PC and 121.7% compared to ME.
Imaging Flow Cytometry as a Quick and Effective Identification Technique of Pollen Grains from Betulaceae, Oleaceae, Urticaceae and Asteraceae
Authors: Gierlicka Iwona , Kasprzyk Idalia , Wnuk Maciej
Published: February 2022

Despite the continuous and intensive development of laboratory techniques, a light microscope is still the most common tool used in pollen grains differentiation. However, microscopy is time-consuming and needs well-educated and experienced researchers. Other currently used techniques can be categorised as images and non-images analysis, but each has certain limitations. We propose a new approach to differentiate pollen grains using the Imaging Flow Cytometry (IFC) technique. It allows for high-throughput fluorescence data recording, which, in contrast to the standard FC, also enables real-time control of the results thanks to the possibility of digital image recording of cells flowing through the measuring capillary. The developed method allows us to determine the characteristics of the pollen grains population based on the obtained fluorescence data, using various combinations of parameters available in the IDEAS software, which can be analysed on different fluorescence channels. On this basis, we distinguished pollen grains both between and within different genera belonging to the Betulaceae, Oleaceae, Urticaceae and Asteraceae families. Thereby, we prove that the proposed methodology is sufficient for accurate, fast, and cost-effective identification and potentially can be used in the routine analysis of allergenic pollen grains.
Flavonoids from Sacred Lotus Stamen Extract Slows Chronological Aging in Yeast Model by Reducing Oxidative Stress and Maintaining Cellular Metabolism
Authors: Tungmunnithum Duangjai , Drouet Samantha , Hano Christophe
Published: February 2022

Nelumbo nucifera is one of the most valuable medicinal species of the Nelumbonaceae family that has been consumed since the ancient historic period. Its stamen is an indispensable ingredient for many recipes of traditional medicines, and has been proved as a rich source of flavonoids that may provide an antiaging action for pharmaceutical or medicinal applications. However, there is no intense study on antiaging potential and molecular mechanisms. This present study was designed to fill in this important research gap by: (1) investigating the effects of sacred lotus stamen extract (LSE) on yeast lifespan extension; and (2) determining their effects on oxidative stress and metabolism to understand the potential antiaging action of its flavonoids. A validated ultrasound-assisted extraction method was also employed in this current work. The results confirmed that LSE is rich in flavonoids, and myricetin-3-O-glucose, quercetin-3-O-glucuronic acid, kaempferol-3-O-glucuronic acid, and isorhamnetin-3-O-glucose are the most abundant ones. In addition, LSE offers a high antioxidant capacity, as evidenced by different in vitro antioxidant assays. This present study also indicated that LSE delayed yeast (Saccharomyces cerevisiae, wild-type strain DBY746) chronological aging compared with untreated control yeast and a positive control (resveratrol) cells. Moreover, LSE acted on central metabolism, gene expressions (SIR2 and SOD2), and enzyme regulation (SIRT and SOD enzymatic activities). These findings are helpful to open the door for the pharmaceutical and medical sectors to employ this potential lotus raw material in their future pharmaceutical product development.
Autologous Stem Cell Transplantation in Multiple Myeloma: Where Are We and Where Do We Want to Go?
Authors: Morè Sonia , Corvatta Laura , Manieri Valentina Maria , Saraceni Francesco , Scortechini Ilaria , Mancini Giorgia , Fiorentini Alessandro , Olivieri Attilio , Offidani Massimo
Published: February 2022

The introduction of high-dose therapy in the 1990s as well as the development of drugs such as thalidomide, lenalidomide, and bortezomib in the 2000s led to an impressive improvement in outcome of patients with multiple myeloma (MM) eligible for autologous stem cell transplantation (ASCT). Clinical trials conducted in the first ten years of the twenty-first century established as standard therapy for these patients a therapeutic approach including induction, single or double ASCT, consolidation, and maintenance therapy. More recently, incorporating second-generation proteasome inhibitors carfilzomib and monoclonal antibody daratumumab into each phase of treatment significantly improved the efficacy of ASCT in terms of measurable residual disease (MRD) negativity, Progression Free Survival (PFS), and Overall Survival (OS). The availability of techniques such as multiparameter flow cytometry (MFC) and next-generation sequencing (NGS) for MRD assessment allowed the design of MRD-based response-adjusted trials that will define, in particular, the role of consolidation and maintenance therapies. In this review, we will provide an overview of the most recent evidence and the future prospects of ASCT in MM patients.
Symmetrically Controlled Design of Twin Alumina-Co Composite Thin Films
Authors: Qi Yunkai , Gu Jianjun , Shi Guochao , Li Xin , Wang Ao , Yang Shumin
Published: February 2022

Self-assembled dual-working electrode electrolytic cells were designed to produce twin alumina-Co composite films with highly symmetrical microstructures using a deflected electric field-assisted alternating current electrodeposition method. The results show that the deposition current density, microstructure, and optical and magnetic properties of the twin composite films exhibit a high degree of symmetry. The distribution of magnetic Co particles in the alumina nanopores can be changed by adjusting the magnitude of the deflected electric field, resulting in a synchronous symmetrical change in the microstructure of the composite films, which enables the fine-tuning of the magneto-optical properties of the twin alumina-Co composite films at the microscopic scale. The current density distribution on the surface of the twin composite films along the direction of the deflected electric field was quantitatively analyzed by theoretical calculations and numerical simulations. The results show that the deposition current density gradually increases from 0.024 A/m2 in region C to 0.056 A/m2 in region A at 6 V deflection voltage. The saturation magnetization intensity gradually increases along the radial direction, which is 118, 130, and 150 kA/m, respectively
Modification Methods of Diamond like Carbon Coating and the Performance in Machining Applications: A Review
Authors: Wang Lijun , Liu Yan , Chen Hui , Wang Mengchao
Published: February 2022

The ideal cutting-tool coating material is characterized by unique chemical and physical properties to achieve excellent cutting performance, a good thermal barrier effect, and a high-quality machined surface. Diamond-like carbon (DLC) coating, as a kind of cutting-tool coating material, has been used in cutting various materials due to its low coefficients of friction and thermal expansion, high hardness, and good chemical inert and thermal conductivity. This article mainly focuses on the modification methods for the DLC coating and their application in machining different materials. Firstly, the methods employed to improve the mechanical properties of DLC coating are reviewed and analyzed, including the multilayer structure design, transition layer, and doping other elements. Secondly, the machining performances of DLC-coated tools in the application of different materials are summarized. This review provides knowledge of modification mechanisms regarding DLC coating and its effects on mechanical properties. For machining different materials, it provides a reference to make a suitable selection and design of DLC coating to obtain better machining performance.
Impact of Biodegradable Materials on the Quality of Plums
Authors: Basiak Ewelina , Linke Manfred , Debeaufort Frédéric , Lenart Andrzej , Geyer Martin
Published: February 2022

Edible starch-based materials have shown a positive impact on quality parameters. In this study, plums (Prunus domestica cv. Jojo) were divided into five groups: a control, two coating treatments (starch and starch-whey protein (80–20%), and two film systems (starch and starch-whey protein (80–20%). Biodegradable packaging, particularly the coating treatment, had no negative effect on color parameters. After 28 days of performed tests, firmness was boosted with starch and starch-whey protein (80–20%) films. With the coated materials, there was no significant difference compared to control group. The lowest transpiration velocity was of plums wrapped in starch films. In the case of respiration rate, no significant difference was observed between the packaging and control samples. After the conducted trials, the weight loss of untreated plums was at 10%, while 5% of weight loss was noticed for plums wrapped in starch materials, and around 6% was noticed for the other materials. Oxygen permeability was higher for S-WP films, the thickness of S and S-WP films were comparable and thickness of starch coating was around 60% higher than S-WP. Both films have an affinity to water and both show typical behavior of water vapor sensitive hydrophilic biopolymers. The starch film with the addition of 20% of proteins increased the resistance of gas exchanges, which represents one of its great benefits.
Study on the Influence of Porosity of the Nacre Layer on the Luster and Surface Roughness of Chinese Large Freshwater Nucleated Pearl
Authors: Zhou Danyi , Lu Taijin , Sun Ruoduan , Zhang Jian
Published: February 2022

The Chinese large freshwater nucleated pearl has become popular for its unique appearance throughout the international jewelry market in recent years. However, its quality evaluation mostly depends on appearance observations, and the influence of the nacre layer’s internal microstructure on the gemstone’s appearance needs further investigation. In this study, light reflectivity, surface height unevenness parameters and porosity of the nacre layer were measured by chroma meter, laser scanning confocal microscope and X-ray computed tomography (μ-CT), which quantitatively described the characteristics of luster, surface roughness and structure compactness of the nacre layer. It was found that the porosity of the nacre layer had a significant influence on appearance features, with an increase of porosity showing more surface blemishes (higher surface roughness parameters) and weaker luster (lower reflectivity). Related results can provide reference for the scientific and quantitative evaluation of pearl quality.
Control of Welding Speed and Current in Machine–Human Cooperative Welding Processes
Authors: Huang Ning , Zhang Junlin , Zhang Tiemin , Zheng Xing , Yan Zhaoyang
Published: February 2022

In intelligent manufacturing processes, the dependence of the weld quality on the welder’s skills in manual welding should be minimized. To better control the welding quality, a machine–human cooperative control system was designed in this study, and a skills learning experiment was conducted to correlate the relationship between welding speed and welding current. The obtained skills were then transferred to the control system to control the human welder to achieve the desired welding speed. In addition, to adjust the welding current to control the welding power, the desired full penetration welds were finally obtained. In the present study, full penetration welds with different welding speeds were obtained in a 304 stainless steel pipe having a wall thickness of 2.03 mm and an outside diameter of 113.5 mm using the machine–human cooperative welding process. The back fusion width was 2.3 to 5.5 mm, which met the quality requirements of the weld. This study provides a research direction for effectively solving the problem of the shortage of welders and for helping unskilled welders to produce quality welds, and lays the foundation for developing the next generation of machine–human cooperative intelligent welding system.
Mode Tracking of Unidirectional Carbon-Based Composite Structures Using Modified Mode Shape Vectors
Authors: Kim Chan-Jung
Published: February 2022

A comparison of mode shapes in isotropic structures can be efficiently performed using the modal assurance criterion (MAC) to determine the similarity between mode shape vectors. However, the unidirectional, carbon-based composite (UCBC) structure shows different dynamic characteristics according to the carbon fiber orientation, even for the same structural configuration. The MAC of a certain mode may result in a poor value for the CBC structures in the case of the existence of the distorted mode shape vector from reinforced carbon fibers. In this study, the mode tracking of the UCBC structure is proposed using the MAC value only under the modified mode shape vector to enhance the MAC value between relevant modes. Because the mode shape vectors of the UCBC structure are altered from those of the isotropic structure owing to the reinforced stiffness along the carbon fiber orientation, the modified mode shape vectors are calculated by multiplying the original vectors with the proposed modification window. The proposed method was verified for simple UCBC structures with five different carbon fiber orientations, from 0° to 90°. The UCBC structures were tracked for five modes, three bending and two torsional, and the results were discussed with reference to earlier study results.
Effects of Electron Correlation inside Disordered Crystals
Authors: Kruchinin Sergei P. , Eglitis Roberts I. , Babak Vitaliy P. , Vyshyvana Iryna G. , Repetsky Stanislav P.
Published: February 2022

We propose a novel approach for characterising the electron spectrum of disordered crystals constructed from a Hamiltonian of electrons as well as phonons and a diagram approach for Green’s function. The system’s electronic states were modelled by means of the multi-band, tight-binding approach. The system’s Hamiltonian is described based on the electron wave functions at the field of the atom nucleus. Our novel approach incorporates the long-range Coulomb interplay of electrons located in different lattice positions. Explicit interpretations of Green’s functions are derived using a diagram method. Equations are obtained for the vertex components for the mass operators of the electron–electron as well aselectron–phonon interplays. A system of equations for the spectrum of elementary excitations in the crystal is obtained, in which the vertex components for the mass operators of electron–electron as well as electron–phonon interplays are renormalised. Thismakes it possible to perform numerical computationsfor the system’s energy spectrum with a predetermined accuracy. In contrast to other approaches in which electron correlations are only taken into account in the limiting cases of an infinitely large and infinitesimal electron density, in this method, electron correlations are described in the general case of an arbitrary density. We obtained the cluster expansion of the density of states (DOS) of the disordered systems. We demonstrate that the addition of the electron-scattering mechanismsto the clusters is decreasing. This happens due to a growing number of positions in the cluster, which hang ontothe small parameter. The computing exactness is fixed by a small parameter for cluster expansion of Green’s functions of electrons as well as phonons.
Effect of Ultrasonic Vibration on Grain Size and Precipitated Phase Distribution of 6061 Aluminum Alloy Welded Joint
Authors: Sui Chufan , Liu Zhengjun , Ai Xingyu , Liu Changjun , Zou Zongxuan
Published: February 2022

To improve the weldability of 6061 aluminum alloy and improve the mechanical properties of welded joints, ultrasonic was introduced into the welding process. The microstructure changes of welded joints under different ultrasonic power were studied, and their effects on the mechanical properties of welded joints were analyzed. The grain size was calculated, and the distribution of precipitated phases was observed by the EBSD technique. The results show that the cavitation and acoustic flow produced by ultrasonic vibration can refine the microstructure of welded joint, reduce the grain size by nearly 50%. It promotes the transition of alloy elements to weld and eliminates the segregation of the strengthening phase to the grain boundary, thus improving the mechanical properties of the welded joint.
Clinical Benefit of Pembrolizumab in Advanced Urothelial Cancer Patients in Real-Life Setting: An Efficacy and Safety Monocentric Study
Authors: Dang Elodie , Vallée Alexandre , Lepage-Seydoux Coralie , Sejean Karine , Bonan Brigitte , Abraham Christine , Beuzeboc Philippe , Ratta Raffaele
Published: February 2022

Background: Pembrolizumab is approved for patients with metastatic urothelial carcinoma (UC) who progressed under platinum therapy. The aim of this study was to assess the efficacy and safety of pembrolizumab in a cohort of real-life UC patients. Methods: This retrospective, observational study included advanced UC patients treated with pembrolizumab in a single institution in France. The co-primary endpoints were overall survival (OS) and progression-free survival (PFS) at 6 months. Secondary endpoints were objective response rate (ORR), duration of response (DOR), disease control rate (DCR) and safety. Results: 78 patients were included in the study. The median OS was 7.3 months (3.8–12.2). The estimated OS rate at 6 months was 61.5% (50.5–72.6). The median PFS was 3.1 months (1.4–7.2). The estimated PFS rate at 6 months was 42.3% (31.1–53.5). The best ORR was 35.9%. The mean DOR was 95.5 days. The DCR was 30.8%. The most common treatment-related adverse events (AEs) of any grade were fatigue (46.2%), diarrhea (11.5%), pruritus (10.3%) and nausea (9.0%). There were no grade 3 AEs that occurred with an incidence of 5% or more. Conclusion: Our results confirmed those of randomized clinical trials concerning the treatment with pembrolizumab in patients with advanced UC that progressed after platinum-based chemotherapy.
Diagnostic Framework of Pelvic Massive Necrosis with Peritonitis following Chemoradiation for Locally Advanced Cervical Cancer: When Is the Surgery Not Demandable? A Case Report and Literature Review
Authors: Sanna Elisabetta , Chiappe Giacomo , Lavra Fabrizio , Nemolato Sonia , Oppi Sara , Macciò Antonio , Madeddu Clelia
Published: February 2022

Concurrent platinum-based chemoradiation (CCRT) is the established treatment for locally advanced cervical cancer and has an acceptable toxicity. Radiation-induced necrosis of the uterus and pelvic tissue is a rare and usually late potential complication. Limited data are available about its management. Here, we describe a case of a patient affected by a locally advanced cervical cancer (stage IVA) who received CCRT, obtaining a partial response with persistence of bladder and rectal infiltration. Unfortunately, after the first brachytherapy dose, the patient developed a worsening clinical picture with fever and altered laboratory data indicative of sepsis; the computed tomography revealed a massive necrosis of the uterus with pelvic abscess and peritonitis. We performed a laparoscopic emergency surgery with removal of the necrotic tissue, supracervical hysterectomy, bilateral-oophorectomy, and abscess drainage. Thereafter, once the severe inflammatory condition was resolved, the patient underwent pelvic exenteration with palliative/curative intent. The postoperative PET/CT was negative for residual disease. However, the patient needed further hospitalization for re-occurrence of peritonitis with multiple abscesses. A careful diagnosis is crucial in locally advanced cervical cancer patients who, after CCRT, present persistent pain and problematic findings at imaging and laboratory parameters. In these cases, radiation-induced necrosis of the pelvis should be suspected. This case helps to clarify the central role of surgery, especially when actinic necrosis leads to complications such as abscess, fistulae, and extensive tissue destruction that cannot be conservatively medically handled. Laparoscopy represents an ideal approach to realizing the correct diagnosis, as well as enabling the performance of important therapeutic surgical procedures.
Interrater and Intrarater Reliability of the EasyForce Dynamometer for Assessment of Maximal Shoulder, Knee and Hip Strength
Authors: Trajković Nebojša , Kozinc Žiga , Smajla Darjan , Šarabon Nejc
Published: February 2022

This study aimed to determine the interrater and intrarater reliability of EasyForce dynamometer for assessing shoulder, knee, and hip muscle strength in healthy young adults. Shoulder, knee, and hip maximal isometric strength were measured using the EasyForce in healthy adults (11 women and 12 men). Three repetitions of shoulder internal rotation, abduction, knee flexion, extension, and hip abduction and adduction were performed. The tests were performed by three raters on the same day. The results showed good to high inter- and intrarater reliability (intraclass correlation coefficient range: 0.63–0.91). Moreover, the absolute reliability of the EasyForce was slightly higher than acceptable for all tests (CV > 10%) except for hip abduction on the right leg (CV = 7.2%). The EasyForce dynamometer can be considered a reliable tool for assessing shoulder internal rotation and abduction, knee extension and flexion, as well as hip abduction and adduction strength. The EasyForce dynamometer showed no differences between the raters’ measurements, which could be of great importance for professionals who want to perform the tests regardless of their strength on the values.
Factors Affecting Patients with Concurrent Deep Neck Infection and Cervical Necrotizing Fasciitis
Authors: Chen Shih-Lung , Chin Shy-Chyi , Wang Yu-Chien , Ho Chia-Ying
Published: February 2022

Deep neck infection (DNI) is a severe disease of the deep neck spaces, which has the potential for airway obstruction. Cervical necrotizing fasciitis (CNF) is a fatal infection of the diffuse soft tissues and fascia with a high mortality rate. This study investigated risk factors in patients with concurrent DNI and CNF. A total of 556 patients with DNI were included in this study between August 2016 and December 2021. Among these patients, 31 had concurrent DNI and CNF. The relevant clinical variables were assessed. In univariate analysis, age (> 60 years, odds ratio (OR) = 2.491, p = 0.014), C-reactive protein (CRP, OR = 1.007, p < 0.001), blood sugar (OR = 1.007, p < 0.001), and diabetes mellitus (DM, OR = 4.017, p < 0.001) were significant risk factors for concurrent DNI and CNF. In multivariate analysis, CRP (OR = 1.006, p < 0.001) and blood sugar (OR = 1.006, p = 0.002) were independent risk factors in patients with concurrent DNI and CNF. There were significant differences in the length of hospital stay and therapeutic management (intubation, tracheostomy, incision and drainage) between DNI patients with and without CNF (all p < 0.05). While there were no differences in pathogens between the DNI alone and concurrent DNI and CNF groups (all p > 0.05), the rate of specific pathogen non-growth from blood cultures was 16.95% (89/525) in the DNI alone group, in contrast to 0% (0/31) in the concurrent DNI and CNF group (p = 0.008). Higher CRP and blood sugar levels were independent risk factors for the concurrence of DNI and CNF. With regard to prognosis, there were significant differences in the length of hospital stay and therapeutic management between the groups with and without CNF. While there were no significant differences in pathogens (all p > 0.05), no cases in the concurrent DNI and CNF group showed specific pathogen non-growth, in contrast to 89/525 patients in the group with DNI alone.
Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction
Authors: Prausmüller Suriya , Spinka Georg , Stasek Stefanie , Arfsten Henrike , Bartko Philipp Emanuel , Goliasch Georg , Hülsmann Martin , Pavo Noemi
Published: February 2022

Background: Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduced ejection fraction (HFrEF) are scarce. The present study aims to evaluate the role of neutrophil CD11b, CD66b and CD64 expression in HFrEF. Methods: A total of 135 HFrEF patients and 43 controls were recruited. Mean fluorescence intensity of the activation/maturation markers CD11b, CD66b and CD64 was measured on neutrophils by flow cytometry. CD10 (neprilysin) expression was simultaneously determined. Results: Neutrophil CD64 expression was higher in HFrEF compared with controls, while CD11b/CD66b levels were similar. Neutrophil CD11b and CD66b showed a significant direct correlation to neutrophil CD10 expression (rs = 0.573, p < 0.001 and rs = 0.184, p = 0.033). Neutrophil CD11b and CD66b correlated inversely with heart failure severity reflected by NT-proBNP and NYHA class (NT-proBNP: rs = −0.243, p = 0.005 and rs = −0.250, p = 0.004; NYHA class: p = 0.032 and p = 0.055), whereas no association for CD64 could be found. Outcome analysis did not reveal a significant association between the expression of CD11b, CD66b and CD64 and all-cause mortality (p = ns). Conclusions: The results underline the potential role of neutrophils in HFrEF disease pathophysiology and risk stratification and should stimulate further research, characterizing subpopulations of neutrophils and searching for key molecules involved in the downward spiral of inflammation and heart failure.