bluuebunny/crossref_metadata_2025 huggingface dataset:
UNTAGGED MUTATION IN RICE GAL4/VP16 TRANSCRIPTIONAL ACTIVATOR FACILITATED-ENHANCER TRAP LINES
Authors: Koerniati Sri
Published: December 2016

An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of gene knock-out, so its insertion in genome is  expected not linked to any dramatic changes in plant phenotypes. Gene  knock-out, leading to lossof- function (LoF) mutation, is a dominant  approach for rice functional genomic studies. The objective of this study was to find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA insertion inducing mutant phenotypes in rice TAFET population. Materials used in this experiment were T1 generation of 270 rice TAFET lines. Eight plants of each were grown in the greenhouse and observed for any mutant phenotypes. Phenotypic, histochemical, Southern
blot analyses were carried out to define a mutant of pSKC66.1- 8e. Result showed that about 10% of the 270 lines produced chlorophyll-deficient  leaves, ranged from yellowish green (viridis), white stripe green zebra-like stripe) to completely white (albino). Albino plants died after two weeks,  whilst white stripe or viridis mutants became normal in the next generation
(T2). Another mutant was pSKC66.1-8e line which had floral dramatic phenotype change with various spikelet shapes and number of organs, and had a single twisted culm. The flower of mutant also had gus gene expression. Plants with wild type did not express gus gene and had six or more straight culms. Molecular, histochemical and phenotypic analyses of this particular line for three generations indicated that mutant phenotype was not due to the T-DNA insertion. Since there was approved that Tos17 is activated during tissue culture and induced mutant phenotype, this line might relate to Tos17 insertion, but it needs further investigation to gain such conclusion.
POTENTIAL USE OF AN EXTRACELLULAR ENZYME OF a-AMYLASE FROM INDIGENOUS INDONESIAN MESOPHILIC BACTERIA
Authors: Lestari Puji , Richana Nur , Masriani Rina
Published: December 2016

Amylase enzyme has a great significance for industrial usages in  Indonesia. However, this enzyme is still imported. The use of bacteria in biotechnological process of industrial products such as enzyme production has stimulated the exploration of extracellular amylase producing  bacteria. This study aimed to identify and analyze the potential use of amylolytic bacterial enzymes for hydrolyzing cassava starch. Two bacterial isolates, i.e. MII-10 and DKW-8 originated from Indonesia soil were identified based on their morphological, physiological and biochemical properties according to the standard protocol. The isolates were then  cultivated on fermentation medium and their growth pattern and  enzymatic assays were observed. The acetone-precipitated crude enzyme harvested based on predetermined cultivation time was used for  enzymatic hydrolysis product characterization on cassava starch using thin layer chromatography (TLC). The results showed that the mesophilic
bacteria isolates (MII-10 and DKW-8) were belonged to Bacillus licheniformis. The maximum bacterial cell growth and enzyme activity were reached at 48 hours after incubation. The MII-10 isolate was found more stable than DKW-8 in producing amylase enzyme. Amylase produced by the MII-10 and DKW- 8 isolates was identified to be an endo-a-amylase as confirmed by oligosaccharides and dextrin of the random hydrolysis
products. Relatively high dextrose equivalence (DE) value of a-amylase of MII-10 (DE of 9.96) suggests that the enzyme is prospective for  saccharification of starchy material in glucose syrup industry.
DEVELOPMENT AND APPLICATION OF 1536-PLEX SINGLE NUCLEOTIDE POLYMORPHISM MARKER CHIP FOR GENOME WIDE SCANNING OF INDONESIAN RICE GERMPLASM
Authors: Utami Dwinita W. , Rosdianti I. , Lestari P. , Satyawan D. , Rijzaani H. , Tasma I M.
Published: December 2016

A successful molecular breeding program requires detailed and comprehensive understanding of the diversity of rice germ-plasm and genetic base of target traits. The objective of this research was to develop the high throughput 1536-SNP chip linked to heading date and yield component traits and used it for genotyping the diverse Indonesian rice germplasm. The genotype data obtained could be used for diversity analysis and genome wide association mapping study. A 1536-SNP genome wide assay was developed using the Illumina’s GoldenGate technology. The SNP markers were selected in the rice genome regions containing heading date and yield component genes or regions where the quantitative trait loci (QTLs) of the two traits were mapped. The developed custom SNP chips were then used for genotyping 467 rice accessions showing diversity in heading dates and yield components. The assay can reliably be used for diversity analysis and mapping genes associated with heading date and yield component traits. For 1536-SNP BIO-RiceOPA-1 custom chip designed, a total of 34.832 SNPs distributed in rice genome particularly in the region of heading date and yield component genes or QTLs were identified. A total of 1536-SNP were selected and confirmed to be used for genotyping analysis. Analysis performance and quality of 1536-SNP BIO-RiceOPA1 showed that 60% (918/1536) of total SNP markers had a good differentiating power in scanning the rice accessions tested (MAF > 0.2). The 1536-SNP genome wide assay Illumina’s GoldenGate designed was useful for diversity analysis and could be used as SNP marker for large scale genotyping in rice molecular breeding involving Indica-Indica, Indica-Japonica and Indica-Tropical Japonica crosses.
DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica)
Authors: Ibrahim Meynarti Sari Dewi , Hartati Rr. Sri , Rubiyo Rubiyo , Purwito Agus , Sudarsono Sudarsono
Published: December 2016

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.
EFFICACY OF ENDOPHYTIC BACTERIA IN REDUCING PLANT PARASITIC NEMATODE Pratylenchus brachyurus
Authors: Harni Rita , Supramana Supramana , Supriadi Supriadi
Published: December 2016

Pratylenchus brachyurus is a major parasitic nematode on patchouli that reduces plant production up to 85%. The use of endophytic bacteria is promising for controlling nematode and promoting plant growth through production of phytohormones and enhancing the availability of soil nutrients. The objective of the study was to evaluate the efficacy of endophytic bacteria to control P. brachyurus on patchouli plant and its influence on plant productions (plant fresh weight and patchouli oil). The study was conducted at Cimanggu Experimental Garden and Laboratory of the Indonesian Spice and Medicinal Crops Research Institute (ISMECRI), Bogor, West Java. The experi-ment was designed in a randomized block with seven treatments and eight replications; each replication consisted of 10 plants. The treatments evaluated were five isolates of endophytic bacteria (Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK and Bacillus subtilis NJ57), synthetic nematicide as a reference, and non-treated plant as a control.  Four-week old patchouli plants of cv. Sidikalang were treated by soaking the roots in suspension of endophytic bacteria (109 cfu  ml-1) for one hour before trans-planting to the field. At one month after planting, the plants were drenched with the bacterial suspension as much as 100 ml per plant. The results showed that applications of the endophytic bacteria could suppress the nematode populations (52.8-80%) and increased plant weight (23.62-57.48%) compared to the control. The isolate of endophytic bacterium Achromobacter xylosoxidans TT2 was the best and comparable with carbofuran.
EFFECTS OF AMELIORANT COMPOSITIONS ON NITROGEN MINERALIZATION AND UPTAKE BY SWEET CORN IN DEGRADED PEATLAND
Authors: Maftu’ah Eni , Ma'as Azwar , Purwanto Benito Heru
Published: December 2016

Peat soil is characterized by poor nitrogen (N) availability. Ameliorants are expected to rectify this problem. This research  aimed to study the effect of ameliorant on N availability and N uptake by sweet corn plant in degraded peatland. The experiment was conducted in the greenhouse in May-July 2011 and on peatland of Kalampangan Village, Palangkaraya, Central Kalimantan in September-December 2011. Burnt peat soil of Kalampangan was used in the greenhouse experiment and sweet corn was used as an indicator plant. The treatments consisted of two factors, i.e. compositions of ameliorants by weight (A1 = 80% chicken manure + 20% dolomite; A2 = 80% local farm weed + 20% dolomite; A3 = 80% mineral soil + 20% dolomite; A4 = 20% chicken manure + 20% local farm weed + 20% residue of Chinese water chestnut (Eleocharis dulcis) + 20% mineral soil + 20% dolomite; and A5 = 19% chicken manure + 71.5% mineral soil + 9.5% dolomite) and rates of those ameliorants (5, 10, 15, 20 and 25 t ha-1). The experiment was arranged in a completely randomized block design with three replications. Data were collected every two weeks for five times. Observations were made on soil pH, available N (NH4+, NO3-), plant height, and N uptake in root and shoot. The results showed that  treatment A1 increased soil pH and availability of NH4+ and NO3-  in peat soils at the maximum vegetative stage. Treatment A1 provided the highest N availability and N uptake by the plant. Field experiment showed that N uptake increased  with the plant yield. Optimum yield of fresh corn cob was obtained from treatment A1 at the rate of 20 t ha-1. This research reconfirms the effectiveness of chicken manure and dolomite as peat soil ameliorant.
VARIATION IN RICE TUNGRO VIRUS TRANSMISSION ABILITY BY GREEN LEAFHOPPER, Nephotettix virescens DISTANT (HOMOPTERA: CICADELLIDAE) ON RICE RESISTANT VARIETIES
Authors: Widiarta I Nyoman , Bastian Adolf , Pakki Syahrir
Published: December 2016

Green leafhopper (GLH), Nephotettix virescens, is the most efficient vector of rice tungro virus disease. The disease is endemic in some provinces of Indonesia and commonly con-trolled using resistant varieties. Resistance of rice varieties to tungro could be classified into resistance to a virus and a vector. The history of GLH resistant varieties adoption affected the GLH adaptation in an area. The study was conducted in the period of 2009-2011 to evaluate the resistance status of five GLH resistant rice variety groups (T0-T4) using survival and transmission test. The GLH populations were collected from 15 tungro endemic provinces in Indonesia. The GLH was then reared in the greenhouse before used for the test. The degree of resistance to tungro viruses was calculated by adding the value of survival (weight x score of survival rate) and virus transmission rate (weight x score of transmission rate). The weights for survival and transmission rate were set to 40 and 60, respectively. The results showed that the rank of resistant variety groups in decreasing order of resistance were T4, T1, T2 and T3. Five variations in GLH transmission efficiency were identified, i.e. 170, 070, 050, 030 and 010. GLH populations from Bali and West Nusa Tenggara were the most efficient vector for rice tungro virus. We concluded that there were diversities in the degree of resistance among GLH resistant varieties. Variation in virus transmission efficiency (biotype) among GLH populations collected from various tungro endemic areas closely related to the history of adoption of rice varieties.
THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK
Authors: Devy Nirmala Friyanti , Yenni Yenni , Hardiyanto Hardiyanto
Published: December 2016

Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
STUDY OF MILLING PROCESS AND ITS EFFECT ON VITAMIN B1 AND FOLIC ACID CONTENTS ON LOWLAND RICE PROMISING LINES
Authors: Indrasari Siti Dewi , Ardhiyanti Shinta Dewi , Abdullah Buang
Published: December 2016

Rice is an important source of vitamin B1 and folic acid, but cultivated rice cultivars contain low of these nutrient. Breeding program had been conducted and several advanced lines with a high vitamin B and folic acid contents were found. This research aimed to study the contents of vitamin B1 and folic acid in the brown rice and milled rice of fifteen promising lowland rice lines. Dried paddy (14% moisture content) were husked in the mini husker (Satake THU 35A) to obtain brown rice and milled rice by using abrasive-type mini polisher (Satake TM-05, 1,450 RPM for 2 minutes). Vitamin B1 and folic acid in the brown rice and milled rice were determined by using HPLC equipped with fluorescence detector for vitamin B1 and UV detector for folic acid. Four out of fifteen rice lines studied showed a unique characteristic of vitamin B1 content in the brown rice and milled rice. Rice lines which have highest vitamin B1 in the brown rice were B10267-4-PN-6-2-3-2-2-2-3-3-2 (3.03 mg 100 g-1), B10876H-MR-2 (2.13 mg 100 g-1), B10531E-KN-1-2-PN-1-4-2 (1.33 mg 100 g-1) and B12411E-RS*-1-2-1 (1.21 mg 100 g-1). However after milling, 43-92% of their vitamin B1 were lost; the least loss was observed in B12411E-RS*-1-2-1 (1.21 mg 100 g-1) which was only  4%. Three out of fifteen rice lines studied has highest folic acid in brown rice, i.e. BP400G-PN-12-3-6 (158.5 µg 100 g-1), B10876H-MR-2 (152.0 µg 100 g-1), and B11742-RS*-2-3-MR-5-5-1-Si-1-3 (100.0 µg 100 g-1). After milling the line B10876H-MR-2 had the highest folic acid content (52.0 µg 100 g-1), much higher than that of Ciherang variety as a control. This study indicates the importance of having a suitable milling instrument to minimize loss of vitamin B1 and folic acid. The study also suggests the importance of creating new rice cultivars that have high nutrient and resistant to milling process.
PREDICTION OF DROUGHT IMPACT ON RICE PADDIES IN WEST JAVA USING ANALOGUE DOWNSCALING METHOD
Authors: Surmaini Elza , Hadi Tri Wahyu , Subagyono Kasdi , Puspito Nanang Tyasbudi
Published: December 2016

Indonesia consistently experiences dry climatic conditions and droughts during El Niño, with significant consequences for rice production. To mitigate the impacts of such droughts, robust, simple and timely rainfall forecast is critically important for predicting drought prior to planting time over rice growing areas in Indonesia. The main objective of this study was to predict drought in rice growing areas using ensemble seasonal prediction. The skill of National Oceanic and Atmospheric Administration’s (NOAA’s) seasonal prediction model Climate Forecast System version 2 (CFSv2) for predicting rice drought in West Java was investigated in a series of hindcast experiments in 1989-2010. The Constructed Analogue (CA) method was employed to produce downscaled local rainfall prediction with stream function (y) and velocity potential (c) at 850 hPa as predictors and observed rainfall as predictant. We used forty two rain gauges in northern part of West Java in Indramayu, Cirebon, Sumedang and Majalengka Districts. To be able to quantify the uncertainties, a multi-window scheme for predictors was applied to obtain ensemble rainfall prediction. Drought events in dry season planting were predicted by rainfall thresholds. The skill of downscaled rainfall prediction was assessed using Relative Operating Characteristics (ROC) method. Results of the study showed that the skills of the probabilistic seasonal prediction for early detection of rice area drought were found to range from 62% to 82% with an improved lead time of 2-4 months. The lead time of 2-4 months provided sufficient time for practical policy makers, extension workers and farmers to cope with drought by preparing suitable farming practices and equipments.
IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS ON CATTLE BREEDS IN INDONESIA USING BOVINE 50K
Authors: Lestari Puji , Rijzaani Habib , Satyawan Dani , Anggraeni Anneke , Utami Dwinita Wikan , Rosdianti Ida , Lutfi Muchamad , Tasma I Made
Published: December 2016

Single nucleotide polymorphisms (SNPs) abundant in bovine genome influence genetic variation in biological mechanism. The study aimed to identify SNPs on Indonesian cattle breeds and analyze their genetic diversity using Bovine 50K SNP chip. Twenty eight "Ongole Grade" (OG) beef cattle and 20 "Holstein Friesian" (HF) dairy cattle were used for the Infinium II assay test. This assay included amplification of genomic DNA, fragmenta-tion, precipitation, resuspension, hybridization, processing bead chip for single-base extension, and imaging at iScan. Data and clusters were analyzed using GenomeStudio software. The Bovine 50K SNP chip containing 54,609 SNPs was observed spanning all chromosomes of bovine genome. Genotyping for the total SNPs was successfull based on Call Rate, GeneCall and GeneTrain scores. Most SNP markers had alleles that shared among the individuals or breeds, or had specific alleles at distinctive frequencies. Minor allele frequency (MAF) spreads equally with intervals of 0-0.5. The breeds of OG and HF tended to be separated in different clusters without considering their genetic history and twin or normal. This result suggests that most individuals are closely related to one another, regardless of the same breed. Some genes identified on chromosomes 3, 4, 5, 7, 13, 17 and 18 were located in the loci/regions that contained SNPs with specific alleles of either HF or OG breed. These SNPs were more powerful for differentiation of beef cattle and dairy cattle than among individuals in the same breed. These SNP variations and genetic relatedness among individuals and breeds serve basic information for cattle breeding in Indonesia.
EVALUATION OF GENETIC DIVERSITY IN CACAO COLLECTED FROM KOLAKA, SOUTHEAST SULAWESI, USING SSR MARKERS
Authors: Rubiyo Rubiyo , Izzah Nur Kholilatul , Sulistiyorini Indah , Tresniawati Cici
Published: December 2016

Kolaka, which is located in Southeast Sulawesi, has long been known as one of cacao production centers in Indonesia. Therefore, many different cacao germplasms can be found in this region. The study aimed to evaluate genetic diversity and relationships of 12 cacao genotypes collected from Kolaka. Genomic DNA was extracted by using a modified CTAB method. Meanwhile, genetic diversity was analyzed based on 16 SSR markers, which then separated by 6% non-denaturing polyacryl-amide gel electrophoresis. The result showed that all of those markers, 14 markers exhibited polymorphism and subsequently used for data analysis using NTSYS and PowerMarker program. About 70 different alleles were generated from 12 cacao genotypes analyzed with an average of 5 alleles per locus. Average value of polymorphism information content (PIC) resulted in this study was 0.59. The cluster analysis using UPGMA method based on the genetic similarity coefficient revealed that all cacao genotypes were separated into three major groups. The first group consisted of five cacao genotypes, the second one held four cacao genotypes, whereas the third group contained three genotypes. This result indicates that three genotypes that clustered separately from the others could be used as a good clonal candidate for cacao breeding program. The information resulted from this present study would be useful for future cacao breeding program, especially in efforts to release a new variety.
GENETIC DIVERSITY OF S3 MAIZE GENOTYPES RESISTANT TO DOWNY MILDEW BASED ON SSR MARKERS
Authors: Muis Amran , Nonci Nurnina , Pabendon Marcia B.
Published: December 2016

The compulsory requirement for releasing new high yielding maize varieties is resistance to downy mildew. The study aimed to determine the level of homozygosity, genetic diversity, and  genetic distance of 30 S3 genotypes of maize. Number of primers to be used were 30 polymorphic SSR loci which are distributed over the entire maize genomes. The S3 genotypes used were resistant to downy mildew with homozygosity level of >80%, genetic distance between the test and tester strains >0.7, and anthesis silking interval (ASI) between inbred lines and tester lines was maximum 3 days. The results showed that 30 SSR primers used were spread evenly across the maize genomes which were manifested in the representation of SSR loci on each chromosome of a total of 10 chromosomes. The levels of polymorphism ranged from 0.13 to 0.78, an average of 0.51, and the number of alleles ranged from 2 to 8 alleles per SSR locus, an average of 4 alleles per SSR locus. The size of nucleotides in each locus also varied from 70 to 553 bp. Cophenetic correlation value (r) at 0.67 indicated that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was less reliable for differentiating genotypes in five groups. Of the total of 30 genotypes analyzed, 17 genotypes had homozygosity level of >80% so it can be included in the hybrid assembly program.
THE EFFECT OF HEATING ON PHYSICOCHEMICAL CHARACTERISTICS OF RICE BRAND OIL
Authors: Indrasari S. Dewi , Koswara Sutrisno , Muchtadi Deddy , Nagara L. Mulyati
Published: December 2016

Rice bran oil has a potential in lowering blood cholesterol. The oil content extracted from rice bran isa influenced by several factors such as raw material and processing method. This research was conducted to study the effect of heating on the physicochemical characteristics of rice bran oil. The study was carried out at the Food Technology and Nutrition Laboratory and Biotechnological Science Laboratory of the Inter University Center, Bogor Agricultural University, from February to MAy 1997. Bran used was from IR64 rice variety obtained from a local milling rice in Tambak Dahan, Binong-Subang, West Java. Rice bran was subjected to different period of heating (0, 15 and 30 minutes) at 121oC by using an autoclave, then the oil was extraxted by using hexane solvent followed by standard processing at room temperature (28-30oC) and 40oC for two hours. The parameters observed were oil content and its quality such as saponification, iodine, acid, peroxide, tiobarbituric acid, and triglyceric acid values as well as oil color. The results showed that oil extracted from rice bran was high, ranging from 9.65 to 11.02%. Heating (sterilizing) of the rice bran for 15 and 30 minutes at 120oC proir to extraction increased the oil content. The Quality of rice bran oil extracted met the standard of AOAC.
CHARACTERISTICS OF RICE SOILS FROM THE TIDAL FLAT AREAS OF MUSI BANYUASIN, SOUTH SUMATRA
Authors: Prasetyo B.H. , Suping S. , H. Subagyo , Mujiono Mujiono , Suhardjo H.
Published: December 2016

Tidal flats in the Musi Banyuasin region that cover more than 200,000 ha are the largest area for agricultural development in South Sumatra Province. Only about a half of this has been used for tidal swamp rice fields, therefore, the other half needs to be developed. To obtain a better understanding of their properties for appropriate soil management, soil characteristics of the area need to be studied. To characterize the soil, thirty-four soil samples from seven soil profiles were analyzed for their chemical and mineralogical composition at the laboratories of the Center for Soil and Agroclimate Research and Development. The results indicate that soils from the tidal flat areas have an aquic soil moisture regime, the upper parts of the soils are mostly ripe, and most of the pedons show the presence of sulfidic materials below 65 cm of the mineral soil surface. The soils are classified as Sulfic Endoaquept (P1, P2), Histic Sulfaquent (P3), Typic Sulfaquept (P4), Fluvaquentic Endoaquept (P5), and Sulfic Hydraquent (P6, P7). Mineral composition of the sand fraction is dominated by quartz, while the clay minerals consist of predominantly kaolinite, mixed with small amount of smectite, illite, quartz, and crystoballite. Organic carbon content is high to very high, potential phosphate content of most pedons ranges from very low to medium, while potential potassium content varies from very low to medium in the upper layers and medium to very high in the bottom layers. Phosphate retention of topsoil sample varies from 56 to 97%, and is positively correlated (r2 = 0.73) with aluminum from amorphous materials. Exchangeable cations are dominated by Mg cation, and in all pedons cation exchange capacity values are medium to very high, and seem to be influenced by organic carbon. Specific chemical properties, particularly soil pH and content of exchangeable aluminum exhibit a significant change about 1-2 months after soil samples were taken from the field. Theoretically, interaction between good water management and fertilizer application are among the choices of management to make these soils productive.
THE CYANOGENIC POTENTIAL OF ROOTS AND LEAVES OF NINETY NINE CASSAVA CULTIVARS
Authors: Hidayat A. , Zuraida N. , Hanarida I.
Published: December 2016

Studies on cyanogenic potential (CP) of roots and leaves of Indonesian cassava germplasm are still inadequate in relation to food toxicity and on human health. The CP of leaves of 99 cassava cultivars was analyzed using picrate paper kits. Effort to reduce CP of cassava leaves by boiling them was also conducted. The results showed that roots and leaves had low and moderate level of CP. There was a significant correlation between the CP of leaves (Y) and roots (X) of 45 cassava cultivars with regression equation Y = 36.214 + 1.3085 X (r = 0.5228). The CP content was high in the young  leaves (241 ppm) and low in the older ones (99 ppm). The proximal portion of the roots had the highest CP content (300 ppm), and that in the distal end was the lowest (56 ppm). The root part close to cortex had highest CP content (550 ppm), whereas the central part was  the lowest (35 ppm). Boiling cassava leaves for 20 minutes significantly reduced the CP up to 75%, indicating that for safety, cassava should be completely processed or cooked. This study implied that CP content should be considered in cassava breeding programs. Forty two of 99 cassava cultivars have CP below 50 ppm which is safe for consumption.
WATERMELON MOSAIC VIRUS OF PUMPKIN (Cucurbita maxima) FROM SULAWESI: IDENTIFICATION, TRANSMISSION, AND HOST RANGE
Authors: Wakmana Wasmo , Kontong M.S. , Teakle D.S. , Persley D.M.
Published: December 2016

A mosaic disease of pumpkin (Cucurbita maxima) was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven different species were inoculated mechanically for host range test. Clarified sap was examined under the electron microscope. Seeds of two pumpkin fruits from two different infected plants were planted and observed for disease transmission up to one-month old seedlings. The mosaic disease was transmitted mechanically from crude sap of different leaf samples to healthy pumpkin seedlings showing mosaic symptoms. The virus also infected eight cucurbits, i.e., cucumber (Cucumis sativus), green melon (Cucumis melo), orange/rock melon (C. melo), zucchini (Cucurbita pepo), pumpkin (Cucurbita maxima), water melon (Citrulus vulgaris), Bennicosa hispida, and blewah (Cucurbita sp.). Aphids  transmitted the disease from one to other pumpkin seedlings. The virus was not transmitted by seed. The mosaic disease of pumpkin at Maros, South Sulawesi, was associated with flexious particles of approximately 750 nm length, possibly a potyvirus, such as water melon mosaic virus rather than papaya ringspot virus or zucchini yellow mosaic virus.
ESTIMATING SOYBEAN PRODUCTION EFFICIENCY IN IRRIGATED AREA OF BRANTAS RIVER BASIN
Authors: Siregar Masdjidin , Sumaryanto Sumaryanto
Published: December 2016

Soybean has an important role in Indonesian diet, especially as source of protein, fat, mineral, and vitamin. Given the relatively stagnant technology, efficiency improvement at farm level would probably be an appropriate way to increase soybean production in the near future. The objective of this paper was to estimate the technical efficiency of soybean production in irrigated area of Brantas river basin and analyze factors affecting technical inefficiency level. The results indicated that the technical efficiency of soybean production in the sites was around 83%. The analysis, however, failed to identify the determinants of technical inefficiency because none of the parameters in the analysis was significant. Further study is required to identify the determinants such that the target groups of extension can be specifically determined. Since K2O fertilizer significantly affected soybean production while P2O5 and N fertilizers did not, the first step to improve the technical efficiency of soybean production was to provide soybean farmers with recommendation regarding balanced amount of fertilizers. The recommendation, however, should be derived from local verification trial in each site.
PERFORMANCES OF TIGER SHRIMP CULTURE IN ENVIRONMENTALLY FRIENDLY PONDS
Authors: Ahmad Taufik , Tjaronge M. , Suryati E.
Published: December 2016

Mangrove ecosystem plays an obvious role in maintaining the biological balance in the coastal environment where shrimp ponds are usually constructed. The removal of mangroves around shrimp ponds has frequently brought about harvest failure. The study evaluated the performance of tiger shrimp culture in ponds provided with water from a water body where there was mangrove vegetation (hereafter mangrove reservoir). Twelve ponds, each measuring 2,500 m2, were filled with seawater from the mangrove reservoir until the water depth of 100 cm and then stocked with 20-40 PL/m2. In the first six ponds, the bottom water was released into the reservoir when the water depth reached 140 cm and then the water depth was maintained at 100 cm. In the second six ponds, the water was released from the ponds until the water depth reached 60 cm and then refilled with reservoir water until a depth of 100 cm. Both treatment ponds received water from the reservoir which also received the wastewater. The feeds for the shrimps were broadcast into the ponds twice a day to meet the 3% shrimp biomass requirement, which adjusted every other week through sampling. The result showed that mangrove  vegetation is capable of removing excessive nutrients, up to 70% for NO3- N and NH4 +-N, reducing PO4 =-P fluctuation, and producing bioactive  compounds. In the second treatment ponds, shrimp mortality started to occur in day 28 and most died by day 54 after stocking due to white spot disease outbreak. Mass mortality took place 54 days after stocking in two out of six of the first treatment ponds.
SOIL MYCOFLORA OF BLACK PEPPER RHIZOSPHERE IN THE PHILIPPINES AND THEIR IN VITRO ANTAGONISM AGAINST Phytophthora capsici L.
Authors: Noveriza Rita , Quimio Tricita H.
Published: December 2016

Foot rot disease of black pepper caused by Phytophthora capsici had been reported in Batangas and Laguna, Philippines. The plant was recovered following the application of crop residue (organic substrate) and intercropping with other crops. This study was aimed to isolate, identify, and determine the soil mycoflora from the rhizosphere of black pepper grown on various cropping patterns in Batangas and Laguna. Antagonistic activity of mycoflora isolates was tested against P. capsici using dual culture technique. The result showed that 149 colonies of soil mycoflora isolated were belonging to 14 genera; three of them, i.e. Penicillium, Paecilomyces and Aspergillus, were the most dominant. All of the mycoflora isolates were able to inhibit the growth of the pathogen. Eighteen of them were the most promising antagonists, based on their inhibition growth of more than 60%. It is suggested that antagonistic mechanism of Mucor isolate (1001), Trichoderma (125, 170, 171, 179, 180, 181), Gliocladium (109), Cunninghamella (165, 168), Mortierella (177), and Aspergillus (106) was space competitor (competition for nutrient) since they rapidly overgrew the pathogen. Aspergillus (67, 79, 81, 83, 108, and 202) isolates inhibited the pathogen apparently by producing antibiotic, whereas Trichoderma (125, 170, 171, 179, 180, and 181) isolates were able to penetrate the hyphae of the pathogen. The organic matter percentage in the soil was significantly correlated with the number of antagonistic mycoflora in rhizosphere (R2 = 0.1094), but the cropping pattern was negatively correlated. This study suggests that organic matter increased antagonistic mycoflora in black pepper rhizosphere, which will reduce severity of the disease.
THE EFFECT OF RICE CULTIVARS ON METHANE EMISSION FROM IRRIGATED RICE FIELD
Authors: Setyanto P. , Rosenani A.B. , Boer R. , Fauziah C.I. , Khanif M.J.
Published: December 2016

Rice plants have been reported to affect methane (CH4) emission from rice fields. The objectives of this study were to determine the effect of rice cultivars on CH4 emission from flooded rice and to develop crop management strategies with low emitting rice cultivars while sustaining high yield. The four rice cultivars studied were Memberamo, Cisadane, IR64, and Way Apoburu. The CH4 emissions were determined in the wet season of 2001/2002 (November-February) using an automated closed chamber technique in an irrigated field condition. Farmyard manure at the rate of 5 t ha-1 was given to the plots to ensure carbon was not limited. Root weight, root length, biomass, and number of tillers were determined at 17, 36, and 57 days after transplanting (DAT). The results showed that the mean CH4 emission was highest in the plot planted with Cisadane (94.8 kg CH4 ha-1), and the lowest with IR64 (37.7 kg CH4 ha-1). The plots treated with emberamo and Way Apoburu resulted an intermediate CH4 emission at the average of 61.1 and 58.9 kg CH4 ha-1, respectively. There was no significant difference in yield between the cultivars tested. The yield of Memberamo, Cisadane, IR64, and Way Apoburu were 5.882, 5.764, 5.873 and 6.065 t ha-1, respectively. Statistical analysis showed that there were no significant differences in the root weight and root length among cultivars. However, Cisadane gave the highest dry matter weight (222 g hill-1) at 57 DAT compared to the other cultivars (175-190 g hill-1). Plant tillers did not show significant differences between the cultivars. Regression analysis showed that CH4 flux was significantly related with root weight, root length, aboveground biomass, and number of plant tillers. This finding shows that the use of selected cultivars, such as IR64, can potentially lower CH4 emission without scarifying yield.
EFFECT OF PHOSPHATE ON NODULE PRIMORDIA OF SOYBEAN (Glycine max Merrill) IN ACID SOILS IN RHIZOTRON EXPERIMENTS
Authors: Waluyo Setiyo Hadi , Lie Tek An , Mannetje Leendert ’t
Published: December 2016

To clarify whether P had a direct or indirect effect on the nodulation  process of soybean grown in acid soils from Sitiung, West Sumatra, Indonesia, a series of rhizotron experiments, with special attention given to formation of nodule primordia, was conducted at Laboratory of  Microbiology, Wageningen University in 1998-2000. It was shown that Ca and P were essential nutrients for root growth, nodule formation, and growth of soybean in the acid soils (Oxisols). Ca increased root growth, number of nodule primordia, nodules, and growth of the soybean plant. This positive effect of Ca was increased considerably by the application of P. Ca and P have a synergistic effect on biological nitrogen fixation (BNF) of soybean in acid soils. Ca is important for the establishment of nodules, whilst P is essential for the development and function of the formed nodules. P increased number of nodule primordia, thus it also has an important role in the initiation of nodule formation. From this study, it can be concluded that Ca and P are the most limiting nutrients for BNF of soybean in the acid soils of Sitiung, West Sumatra, Indonesia.
DIVERSITY AND ABUNDANCE OF MITES IN A MANDARIN CITRUS ORCHARD IN WEST SUMATRA
Authors: Affandi Affandi , Corpuz-Raros Leonila A.
Published: December 2016

Some of the most destructive pests in citrus orchards are spider mites. Monitoring prior applied pest control is a standard procedure for integrated pest management (IPM). The study aimed to survey and determine the distribution, abundance, and diversity of phytophagous, predatory, and detritivorous mites at different stages of development of arboreal plant parts, weeds and litter in the citrus orchard. A periodical sampling survey method was conducted on six growth stages of mandarin citrus, weeds and litter of the Indonesian Fruits Research Institute experimental field in Solok, West Sumatra in December 2003 to June 2004. All mites attached on each growth stage of citrus, weeds and litter were trapped and mounted on slide for identification purposes. Identification of the mites was conducted in the Acarology Laboratory, Department of Entomology, University of the Philippines at Los Banos in July-October 2004. The results showed that a very rich mite fauna of 130 species was found in the arboreal parts of citrus crops, weeds and litter below the canopy of the citrus orchard. Among the trophic groups, detritivorous mites were the most abundant, followed by the predators, phytophages, and those unknown feeding habit. Among the habitats, weeds harbored the most mite species followed by litter and arboreal parts of the citrus trees. The flush growth stage had the least diverse mite fauna with index diversity (H’) of 1.27, while the most diverse (H’ = 2.01) was found at fruit development phase II. Result of this research was useful in determining proper time for controlling phytophagous mites. Besides, the result also provides important information on potential predatory mites that can be used as biological control agents. Furthermore, the study implies the importance of maintaining cover crops of weeds and litter beneath the citrus trees as refuge and source of alternate prey for predators which suppressed populations of phytophagous mites.
BIOLOGY AND INTRINSIC GROWTH RATE OF EARWIG (Euborellia annulata)
Authors: Nonci Nurnina
Published: December 2016

Earwig (Euborellia annulata) is a potential predator of corn borer (Ostrinia furnacalis), one of the most important pests of corn. To include the use of predator in integrated pest management (IPM) to control the pest, it is necessary to understand the basic information of the predator. This study aimed to know biology and intrinsic growth rate of the predator feed on an artificial media (dog food). Ten pairs of newly emerging adults of the predator were placed in a small plastic container containing a mixture of soil and sand (1:1 v/v) supplemented with an artificial food. The average temperature and relative humidity during the study were kept at 27.9-30.3oC and 76.7-92.3%, respectively. The biological aspects of the predator evaluated were number of eggs laid, hatched, and died, as well as its oviposition period and adult mortality. The intrinsic growth rate was studied from a group of 200 newly laid eggs and results were analyzed based on the method of Birch. The biological aspects study showed that number of eggs laid by a single female of E. annulata was 86-166, which were laid five times in a group of 9-45 eggs. The nymph consists of five instars. Length of nymphs varied ranging from 4 to 13 mm depends on their instar. The fifth instar nymph period was 4-6 days for female and 2-3 days for male. The period of first mating was shorter; the shortest was 2 minutes and the longest one was 70 minutes. Both male and female were able to do mating several times at an interval of several seconds or minutes. Pre-oviposition period was 6-13 days. The eggs were deposited five times, the first, second, third, fourth, and fifth oviposition period were 7-22 days, 7-21 days, 7-18 days, 11-18 days, and 11-21 days, respectively. The oviposition period was 32-59 days and postoviposition period was 21-51 days. Ratio between male and female was 1.4:1.0. The average natural mortality of E. annulata was 10.5% which means that 89.5% of deposited eggs hatched became nymphs. The study also revealed that the intrinsic growth rate (r) of the predator was 0.0772, net reproductive rate (R0) 80.16, limited growth rate (λ) 1.17, nymph stage period 39-46 days, and adult longevity of male and female were 57-75 and 61-89 days, respectively. The study implies that E. annulata is potential to be incorporated in IPM for controlling corn borer.
EFFECT OF BIO FERTILIZER ON MATURE OIL PALM IN NORTH SUMATRA AND RIAU
Authors: Adiwiganda Y Taryo , Tarigan Balaman , Purba Berani
Published: December 2016

Chemical fertilizers have routinely been applied at oil palm plantations to maintain soil fertility. Due to the increase of chemical fertilizer prices, some efforts have been done at such as to reduce cost and increase efficiency in chemical fertilizer application at oil palm plantations include the use of bio fertilizer (BF). The objective of experiment was to evaluate the effect of reducing oil palm standard dosage of chemical fertilizers combined with BF on mature oil palm yield. The experiment was carried out in 2002-2003 covering an area of 4367 ha at PTPN IV in North Sumatra and of 515.58 ha at PTPN V in Riau. The planting material was Dura x Pasifera variety and planted in 1980-1995. A randomized block design was used having three fertilizer treatments: (A) 100% chemical fertilizer (normal dosage), (B) 75% chemical fertilizer + BF, and (C) 50% chemical fertilizer + BF. The replications at PTPN IV and V were 11 and 7, respectively. The BF was applied firstly in May 2002. Dosage of BF was 250 g tree-1 yr-1. The BF was given in pockets with four pockets per palm tree. The parameters observed were fresh fruit bunch (FFB) weight, average of bunch weight, and average of bunch number. Results of the experiments showed that palm yield treated with 75% chemical fertilizer + BF and 50% chemical fertilizer + BF were both not significantly different with palm yield treated with 100% chemical fertilizer. The application of 75% chemical fertilizer + BF and 50% chemical fertilizer + BF gave benefit of Rp509,093 and Rp1,231,535 ha-1 yr-1, respectively. Technically, nutrients given as chemical fertilizers using combination of 75% chemical fertilizer + BF are still higher than the nutrients transported into 26 tons palm FFB ha-1 yr-1. Fertilizer treatment combination of 50% chemical fertilizer + BF will technically be safe only for palm yield of <16 tons palm FFB ha-1 yr-1, since nutrients given as fertilizer are still higher than those transported into the palm yield.
SELECTION RESPONSE FOR INCREASED GRAIN YIELD IN TWO HIGH OIL MAIZE SYNTHETICS
Authors: Mejaya Made J. , Lambert R.J.
Published: December 2016

Selection for increased oil level in maize showed the increase was associated with decrease in starch concentration, kernel weight, and grain yield. The study was conducted with the objectives: (1) to evaluate response to six cycles for increased grain yield in the high oil maize Alexho Elite (AE: 60-90 g kg-1 oil concentration) and Ultra High Oil (UHO: 100-140 g kg-1 oil concentration) using inbred tester B73; (2) to measure responses to selection for increased grain yield with changes in yield components; and (3) to determine a suitable tester. Previously the two synthetics had been selected for oil concentration. After six cycles, the six genotypes i.e. AE C0, AE C3, AE C6, UHO C0, UHO C3, and UHO C6 were testcrossed to B73, LH185, and LH202 inbreds (40 g kg-1 oil concentration) to a total of 18 testcrosses. Two field experiments were used to evaluate selection in AE and UHO testcrosses. The study showed selection using inbred tester B73 in AE and UHO was effective in increasing grain yield of AE testcrosses without changing (i.e. decreasing) oil and protein concentrations. AE testcrosses produced higher grain yield and greater selection response for grain yield than UHO testcrosses. LH185 was best for grain yield in AE and UHO testcrosses. Increase in grain yield in most of the testcrosses was associated with increases in starch concentration, kernel weight, kernel number, and grain weight.