bluuebunny/crossref_metadata_2025 huggingface dataset:
Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma
Authors: Mika Aleksander , Luelling Sarah E. , Pavek Adriene , Nartker Christopher , Heyneman Alexandra L. , Jones Kevin B. , Barrott Jared J.
Published: March 2019

YM155 is an anti-cancer therapy that has advanced into 11 different human clinical trials to treat various cancers. This apoptosis-inducing therapy indirectly affects the protein levels of survivin (gene: Birc5), but the molecular underpinnings of the mechanism remain largely unknown. Synovial sarcoma is a rare soft-tissue malignancy with high protein expression of survivin. We investigated whether YM155 would be a viable therapeutic option to treat synovial sarcoma. YM155 therapy was applied to human synovial sarcoma cell lines and to a genetically engineered mouse model of synovial sarcoma. We discovered that YM155 exhibited nanomolar potency against human synovial sarcoma cell lines and the treated mice with synovial sarcoma demonstrated a 50% reduction in tumor volume compared to control treated mice. We further investigated the mechanism of action of YM155 by looking at the change of lysine modifications of the histone tails that were within 250 base pairs of the Birc5 promoter. Using chromatin immunoprecipitation (ChIP)-qPCR, we discovered that the histone epigenetic marks of H3K27 for the Birc5 promoter changed upon YM155 treatment. H3K27me3 and H3K27ac increased, but the net result was decreased Birc5/survivin expression. Furthermore, the combination of molecular events resulted in caspase 3/7/8 upregulation and death of the sarcoma cells.
Indocyanine Green—Mediated Photodynamic Therapy Reduces Methicillin-Resistant Staphylococcus aureus Drug Resistance
Authors: Wong Tak-Wah , Liao Shu-Zhen , Ko Wen-Chien , Wu Chi-Jung , Wu Shin Bei , Chuang Yin-Ching , Huang I-Hsiu
Published: March 2019

Methicillin-resistant Staphylococcus aureus (MRSA) skin-wound infections are associated with considerable morbidity and mortality. Indocyanine green (ICG), a safe and inexpensive dye used in clinical imaging, can be activated by near-infrared in photodynamic therapy (PDT) and photothermal therapy (PTT) to effectively kill MRSA. However, how this treatment affects MRSA drug sensitivity remains unknown. The drug-sensitivity phenotypes, bacterial growth rate, and cell-wall thickness of three MRSA strains were analyzed after ICG-PDT. Drug-resistant gene expressions were determined by polymerase chain reaction (PCR) and quantitative reverse transcription (qRT)-PCR. Related protein expressions were examined with immunoblotting. Drug sensitivity was further evaluated in animal models. MRSA that survived the treatment grew faster, and the cell wall became thinner compared to parental cells. These cells became more sensitive to oxacillin, which was partly related to mecA complex gene deletion. Skin necrosis caused by ICG-PDT-treated MRSA infection was smaller and healed faster than that infected with parental cells. With oxacillin therapy, no bacteria could be isolated from mouse lung tissue infected with ICG-PDT-treated MRSA. ICG-PDT drives MRSA toward an oxacillin-sensitive phenotype. It has the potential to develop into an alternative or adjuvant clinical treatment against MRSA wound infections.
Predictive Models of Double-Vibropolishing in Bowl System Using Artificial Intelligence Methods
Authors: Alcaraz Joselito Yam II , Ahluwalia Kunal , Yeo Swee-Hock
Published: March 2019

Vibratory finishing is a versatile and efficient surface finishing process widely used to finish components of various functionalities. Research efforts were focused in fundamental understanding of the process through analytical solutions and simulations. On the other hand, predictive modelling of surface roughness using computational intelligence (CI) methods are emerging in recent years, though CI methods have not been extensively applied yet to a new vibratory finishing method called double-vibropolishing. In this study, multi-variable regression, artificial neural networks, and genetic programming models were designed and trained with experimental data obtained from subjecting rectangular Ti-6Al-4V test coupons to double vibropolishing in a bowl system configuration. Model selection was done by comparing the mean-absolute percentage error and r-squared values from both training and testing datasets. Exponential regression was determined as the best model for the bowl double-vibropolishing system studied with a Test MAPE score of 6.1% and a R-squared score of 0.99. A family of curves was generated using the exponential regression model as a potential tool in predicting surface roughness with time.
Metformin Pharmacogenetics: Effects of SLC22A1, SLC22A2, and SLC22A3 Polymorphisms on Glycemic Control and HbA1c Levels
Authors: AL-Eitan Laith N. , Almomani Basima A. , Nassar Ahmad M. , Elsaqa Barakat Z. , Saadeh Nesreen A.
Published: March 2019

Type 2 diabetes mellitus (T2DM) constitutes a major portion of Jordan’s disease burden, and incidence rates are rising at a rapid rate. Due to variability in the drug’s response between ethnic groups, it is imperative that the pharmacogenetics of metformin be investigated in the Jordanian population. The objective of this study was to investigate the relationship between twenty-one single nucleotide polymorphisms (SNPs) in the SLC22A1, SLC22A2, and SLC22A3 genes and their effects on metformin pharmacogenetics in Jordanian patients diagnosed with type 2 diabetes mellitus. Blood samples were collected from 212 Jordanian diabetics who fulfilled the inclusion criteria, which were then used in SNP genotyping and determination of HbA1c levels. The rs12194182 SNP in the SLC22A3 gene was found to have a significant association (p < 0.05) with lower mean HbA1c levels, and this association more pronounced in patients with the CC genotype (i.e., p-value was significant before correcting for multiple testing). Moreover, the multinomial logistic regression analysis showed that SNP genotypes within the SLC22A1, SLC22A2, and SLC22A3 genes, body mass index (BMI) and age of diagnosis were significantly associated with glycemic control (p < 0.05). The results of this study can be used to predict response to metformin and other classes of T2DM drugs, making treatment more individualized and resulting in better clinical outcomes.
The Effect of Phase Angle on the Thermo-Mechanical Fatigue Life of a Titanium Metal Matrix Composite
Authors: Dyer Ashley , Jones Jonathan , Cutts Richard , Whittaker Mark
Published: March 2019

The thermo-mechanical fatigue (TMF) behaviour of a Ti-6Al-4V matrix composite reinforced with SCS-6 silicon carbide fibres (140 μm longitudinal fibres, laid up hexagonally) has been investigated. In-phase and out-of-phase TMF cycles were utilized, cycling between 80–300 °C, with varying maximum stress. The microstructure and fracture surfaces were studied using electron backscatter diffraction (EBSD), energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), profilometry, and optical microscopy. The results have shown the damaging effect of out-of-phase cycling with crack initiation occurring earlier than in in-phase tests and crack propagation rates being accelerated in out-of-phase cycles. Fatigue crack initiation has been shown to be sensitive to crystallographic texture in the cladding material and thermo-mechanical fatigue test results can be considered according to a previously proposed conceptual framework for the interpretation of metal matrix composite fatigue.
Damage from Coexistence of Ferroelectric and Antiferroelectric Domains and Clustering of O Vacancies in PZT: An Elastic and Raman Study
Authors: Cordero Francesco , Buixaderas Elena , Galassi Carmen
Published: March 2019

It is often suggested that oxygen vacancies (V O ) are involved in fatigue and pinning of domain walls in ferroelectric (FE) materials, but generally without definite evidence or models. Here the progress of damage induced by the coexistence of FE and antiferroelectric (AFE) domains in the absence of electric cycling is probed by monitoring the Young’s modulus, which may undergo more than fourfold softenings without significant changes in the Raman spectra, but may end with the disaggregation of PZT with ∼5% Ti. At these compositions, the FE and AFE phases coexist at room temperature, as also observed with micro-Raman, and hence the observations are interpreted in terms of the aggregation of V O at the interfaces between FE and AFE domains, which are sources of internal electric and stress fields. The V O would coalesce into planar defects whose extension grows with time but can be dissolved by annealing above 600 K, which indeed restores the original stiffness. The observed giant softening is interpreted by assimilating the planar aggregations of V O to flat inclusions with much reduced elastic moduli, due to the missing Zr/Ti−O bonds. A relationship between the coalescence of a fixed concentration of V O into planar defects and softening is then obtained from the existing literature on the effective elastic moduli of materials with inclusions of various shapes.
Fabrication of Nanopillar Crystalline ITO Thin Films with High Transmittance and IR Reflectance by RF Magnetron Sputtering
Authors: Dong Ling , Zhu Guisheng , Xu Huarui , Jiang Xupeng , Zhang Xiuyun , Zhao Yunyun , Yan Dongliang , Yuan Le , Yu Aibing
Published: March 2019

Nanopillar crystalline indium tin oxide (ITO) thin films were deposited on soda-lime glass substrates by radio frequency (RF) magnetron sputtering under the power levels of 100 W, 150 W, 200 W and 250 W. The preparation process of thin films is divided into two steps, firstly, sputtering a very thin and granular crystalline film at the bottom, and then sputtering a nanopillar crystalline film above the bottom film. The structure, morphology, optical and electrical properties of the nanopillar crystalline ITO thin films were investigated. From X-ray diffraction (XRD) analysis, the nanopillar crystalline thin films shows (400) preferred orientation. Due to the effect of the bottom granular grains, the crystallinity of the nanopillar crystals on the upper layer was greatly improved. The nanopillar crystalline ITO thin films exhibited excellent electrical properties, enhanced visible light transmittance and a highly infrared reflectivity in the mid-infrared region. It is noted that the thin film deposited at 200 W showed the best combination of optical and electrical performance, with resistivity of 1.44 × 10−4 Ω cm, average transmittance of 88.49% (with a film thickness of 1031 nm) and IR reflectivity reaching 89.18%.
Study on Dynamic Response Characteristics of Saturated Asphalt Pavement under Multi-Field Coupling
Authors: Sun Yazhen , Guo Rui , Gao Lin , Wang Jinchang , Wang Xiaochen , Yuan Xuezhong
Published: March 2019

To study the dynamic response of saturated asphalt pavement under moving load and temperature load, 3-D finite element models for asphalt pavements with hydro-mechanical coupling and thermal-hydro-mechanical coupling were built based on the porous media theory and Biot theory. First, the asphalt pavement structure was considered as an ideal saturated fluid–solid biphasic porous medium. Following this, the spatial distribution and the change law of the pore-water pressure with time, the transverse stress, and the vertical displacement response of the asphalt pavement under different speeds, loading times, and temperatures were investigated. The simulation results show that both the curves of the effective stress and the pore-water pressure versus the external loads have similar patterns. The damage of the asphalt membrane is mainly caused by the cyclic effect of positive and negative pore-water pressure. Moreover, the peak value of pore-water pressure is affected by the loading rate and the loading time, and both have positive exponential effects on the pore-water pressure. In addition, the transverse stress of the upper layer pavement is deeply affected by the temperature load, which is more likely to cause as transverse crack in the pavement, resulting in the formation of temperature cracks on the road surface. The vertical stress at the middle point in the upper layer of the saturated asphalt pavement, under the action of the temperature load and the driving load, shows a single peak.
UV Irradiated Graphene-Based Nanocomposites: Change in the Mechanical Properties by Local HarmoniX Atomic Force Microscopy Detection
Authors: Guadagno Liberata , Naddeo Carlo , Raimondo Marialuigia , Speranza Vito , Pantani Roberto , Acquesta Annalisa , Carangelo Anna , Monetta Tullio
Published: March 2019

Epoxy based coatings are susceptible to ultra violet (UV) damage and their durability can be significantly reduced in outdoor environments. This paper highlights a relevant property of graphene-based nanoparticles: Graphene Nanoplatelets (GNPs) incorporated in an epoxy-based free-standing film determine a strong decrease of the mechanical damages caused by UV irradiation. The effects of UV light on the morphology and mechanical properties of the solidified nanocharged epoxy films are investigated by Atomic Force Microscopy (AFM), in the acquisition mode “HarmoniX.” Nanometric-resolved maps of the mechanical properties of the multi-phase material evidence that the incorporation of low percentages, between 0.1% and 1.0% by weight, of graphene nanoplatelets (GNPs) in the polymeric film causes a relevant enhancement in the mechanical stability of the irradiated films. The beneficial effect progressively increases with increasing GNP percentage. The paper also highlights the potentiality of AFM microscopy, in the acquisition mode “HarmoniX” for studying multiphase polymeric systems.
Inspection of Reactor Steel Degradation by Magnetic Adaptive Testing
Authors: Vértesy Gábor , Gasparics Antal , Szenthe Ildikó , Gillemot Ferenc , Uytdenhouwen Inge
Published: March 2019

Degradation of nuclear pressure vessel steel materials, 15Kh2NMFA type and A508 Cl2 type (definition is given in the text) were investigated by a novel magnetic nondestructive testing method, so-called Magnetic Adaptive Testing (MAT), which is based on systematic measurement and evaluation of minor magnetic hysteresis loops. The measured samples were thermally treated by a special step cooling procedure, which generated structural changes in the material. It was found that this type of degradation can be easily followed by magnetic measurements. Charpy impact test were also performed and the results were compared with the magnetic parameters. In case of 15Kh2NMFA steel, a good, reliable and closely linear correlation was found between magnetic descriptors and transition temperature.
A Semi-Empirical Deflection-Based Method for Crack Width Prediction in Accelerated Construction of Steel Fibrous High-Performance Composite Small Box Girder
Authors: Gautam Bishnu Gupt , Xiang Yi-Qiang , Qiu Zheng , Guo Shu-Hai
Published: March 2019

Accelerated construction in the form of steel–concrete composite beams is among the most efficient methods to construct highway bridges. One of the main problems with this type of composite structures, which has not yet been fully clarified in the case of continuous beam, is the crack zone initiation that gradually expands through the beam width. In the current study, a semi-empirical model was proposed to predict the size of cracks in terms of small box girder deflection and intensity of load applied on the structure. To this end, a set of steel–concrete composite small box girders were constructed by the use of steel fibrous concrete and experimentally tested under different caseloads. The results were then used to create a dataset of the box girder response in terms of beam deflection and crack width. The obtained dataset was then utilized to develop a simplified formula providing the maximum width of cracks. The results showed that the cracks initiated in the hogging moment region when the load exceeded 80 kN. Additionally, it was observed that the maximum cracked zone occurred in the center of the beam due to the maximum negative moment. Moreover, the crack width of the box girder at different loading cases was compared with the test results obtained from the literature. A good agreement has been found between the proposed model and experiment results.
Influence of the Loading Speed on the Ductility Properties of Corroded Reinforcing Bars in Concrete
Authors: Bazán Angela Moreno , González María de las Nieves , Alberti Marcos G. , Gálvez Jaime C.
Published: March 2019

In this work 144 reinforcing bars of high-ductility steel named B500SD were subjected to an accelerated corrosion treatment and then tested under tension at different loading speeds in order to assess the effect of corrosion on the ductility properties of the rebars. Results showed that the bars with a corrosion level as low as the one reducing the steel mass by 1% gave rise to a significant degradation on the ductility properties when a high loading speed was applied in tensile tests. In that case, the equivalent steel concept is useful to reduce the destabilising effect. Thus, the research significance lies in the assessment of the influence of the loading speed at which the tensile test is performed for the reinforcement bars that largely depends of the ductility criteria used.
First-Principles Study on the Mechanical Properties and Electronic Structure of V Doped WCoB and W2CoB2 Ternary Borides
Authors: Zhang Tong , Yin Haiqing , Zhang Cong , Zhang Ruijie , Jiang Xue , Zheng Qingjun , Qu Xuanhui
Published: March 2019

For the purpose of exploring new hard materials and doping methods, the structural, mechanical and electronic properties of WCoB and W2CoB2 ternary boride were investigated with 0, 8.33, 16.67, 25 and 33.33 at.% V doping content and W2CoB2 with 0, 5, 10, 15 and 20 at.% V doping content by first-principle calculations. The cohesive energy, impurity formation energy and formation energy indicate the structural stability of V doped WCoB and W2CoB2. The elastic constants and mechanical properties imply that V doping leads to the decrement of shear modulus and the increment of ductility. Two different kinds of hardness models verify that V doping contributes to the decrement of hardness, which is closely related to shear modulus. The electronic structure is analyzed by DOS (density of states), PDOS (partial density of states) and charge density difference, which indicate the formation of weaker B–V covalent bonds, W–V and W–W metallic bonds lead to the decrement of mechanical properties. Compared with previous studies of Cr, Mn doped WCoB and W2CoB2, V doping leads to worse mechanical properties and hardness, indicating V may not be a suitable choice of doping transition elements.
Flexible Carbon Nanotube-Based Polymer Electrode for Long-Term Electrocardiographic Recording
Authors: Chi Miao , Zhao Jingjing , Dong Ying , Wang Xiaohao
Published: March 2019

The long-term monitoring of electrocardiogram (ECG) is critical for the accurate diagnosis and tracking of cardiovascular diseases (CVDs). However, the commercial Ag/AgCl electrode is not suitable for long-term monitoring due to skin allergies and signal degradation, caused by the conductive gel drying over time. In this paper, a flexible gel-free electrode, composed of a multi-wall carbon nanotube (MWCNT) and polydimethylsiloxane (PDMS), is proposed for long-term wearable ECG monitoring. To achieve uniform dispersion of MWCNTs in viscous PDMS, we developed a novel parallel solvent-assisted ultrasonic dispersion method, wherein the organic solvent n–Hexane served as a dispersion to avoid MWCNT aggregates. The properties of the MWCNT/PDMS electrode were assessed through structural characterization, contact impedance tests, ECG measurements, and biocompatibility tests. When the MWCNT weight fraction reached 5.5 wt%, the skin-electrode contact impedance of the MWCNT/PDMS electrode was lower than that of the Ag/AgCl electrode below 100 Hz. In daily ECG monitoring, the MWCNT/PDMS electrodes showed superior performance against motion artifact compared to the Ag/AgCl electrode. After seven days of wearing the MWCNT/PDMS electrode, ECG signals did not degrade and no side effects, such as skin redness and swelling, were observed. Thus, this electrode could enable long-term ECG monitoring in wearable healthcare systems.
CH4 Adsorption Probability on GaN(0001) and (000−1) during Metalorganic Vapor Phase Epitaxy and Its Relationship to Carbon Contamination in the Films
Authors: Kusaba Akira , Li Guanchen , Kempisty Pawel , von Spakovsky Michael R. , Kangawa Yoshihiro
Published: March 2019

Suppression of carbon contamination in GaN films grown using metalorganic vapor phase epitaxy (MOVPE) is a crucial issue in its application to high power and high frequency electronic devices. To know how to reduce the C concentration in the films, a sequential analysis based on first principles calculations is performed. Thus, surface reconstruction and the adsorption of the CH4 produced by the decomposition of the Ga source, Ga(CH3)3, and its incorporation into the GaN sub-surface layers are investigated. In this sequential analysis, the dataset of the adsorption probability of CH4 on reconstructed surfaces is indispensable, as is the energy of the C impurity in the GaN sub-surface layers. The C adsorption probability is obtained based on steepest-entropy-ascent quantum thermodynamics (SEAQT). SEAQT is a thermodynamic ensemble-based, non-phenomenological framework that can predict the behavior of non-equilibrium processes, even those far from equilibrium. This framework is suitable especially when one studies the adsorption behavior of an impurity molecule because the conventional approach, the chemical potential control method, cannot be applied to a quantitative analysis for such a system. The proposed sequential model successfully explains the influence of the growth orientation, GaN(0001) and (000−1), on the incorporation of C into the film. This model can contribute to the suppression of the C contamination in GaN MOVPE.
Effects of High Temperature on the Burst Process of Carbon Fiber/PVA Fiber High-Strength Concretes
Authors: Cao Rui-dong , Yang Hui-wei , Lu Guo-yun
Published: March 2019

This paper carried out burst tests on the carbon and polyvinyl alcohol (PVA) fiber high-strength concrete specimens to investigate the effects of fiber type, fiber content, water content, heating rate and test specimen size on the burst, and the whole burst process of fiber-high concrete was photographed and recorded. The results indicated that fiber addition will improve the high temperature burst behavior of the high-strength concrete, and the performance of PVA is greatly different from that of carbon fiber. The water content and heating rate have little influence on the burst of the PVA test specimen, but they will greatly affect the carbon fiber test specimen. The size of the test specimen has a great influence on the burst. For the PVA concrete test specimen, the large size test specimen bursts on the surface; as for the carbon fiber test specimen, the large size test specimen delays the initial burst time, but the burst becomes fiercer.
An Approach to the Uniform Dispersion of Graphene Nanosheets in Powder Metallurgy Nickel-Based Superalloy
Authors: Gao Yu-Xi , Zou Jin-Wen , Wang Xiao-Feng , Yang Jie , Li Zhuo , Zhu Yan-Yan , Wang Hua-Ming
Published: March 2019

In this paper, a wet-chemical based method was adopted to acquire the uniform dispersion of graphene nanosheets (GNSs) in a powder metallurgy nickel-based superalloy (FGH96) to fabricate a new GNSs reinforced FGH96 metal matrix composite. The surface of the FGH96 powder was modified using a hydrophilic surfactant named polyvinyl alcohol (PVA), which has good wettability and strong hydrogen bonding between the –OH groups of PVA and oxygen groups of GNSs such as –COOH, –CHO, and –OH. It was shown that the GNSs displayed much better dispersion uniformity on the PVA modified FGH96 powder than the unmodified one. The existence of PVA improved the adsorptive capacity of the GNSs attached on the powder surface and prevented the agglomeration in the following thermal preparation process. Consequently, the micro-hardness of PVA modified composite with 0.1 wt.% GNSs reached 497.9 HV, 3.4% higher than the unmodified FGH96 alloy. Therefore, this preparation process could act as the foundation of a common strategy for the fabrication of GNSs in metal matrix composites with good dispersion uniformity, which may have great potential application in aerospace applications.
Bending Properties of Mg Alloy Tailored Arc-Heat-Treated Blanks
Authors: Ren Daxin , Zeng Fanyu , Liu Liming , Zhao Kunmin
Published: March 2019

Tailored heat-treated blank is a special kind of sheet, and the plastic forming ability can be improved. In this work, the poor room-temperature plasticity of a tailored magnesium alloy blank was address through arc heat treatment. The formability of the material was enhanced through local modification with arc pretreatment. The plasticity of the tailored arc-heat-treated blank was verified through the V-bending test. The microstructure and mechanical properties of the blank were tested, and the mechanisms underlying its improved deformability were analyzed. The bendability of the blank first increased and then decreased as heat input increased. The maximum V-bending ability of the arc-heat-treated blank increased by 88% relative to that of the untreated blank. Although springback decreased under increasing heat input, the local strength and elastic modulus of the alloy blank were equivalent to those of the base metal. This result indicated that the springback resistance of the material did not improve. The back of the blank treated under the optimal parameters comprised heat-affected zones with good plasticity. Recrystallization and grain growth occurred in the heat-affected zones. The blank exhibited reduced hardness and improved malleability. When the heat input was further increased, however, a semi-melting zone formed on the lower surface of the blank. The formation of this zone resulted in the precipitation of intermetallic compounds from the crystal phase and increased the hardness of the blank. It also decreased the plasticity and malleability of the blank.
Graphene-Based Inks for Printing of Planar Micro-Supercapacitors: A Review
Authors: Sang Tran Tuan , Dutta Naba Kumar , Roy Choudhury Namita
Published: March 2019

Micro-supercapacitors have recently emerged as promising microscale power sources for portable and wearable microelectronics. However, most reported planar micro-supercapacitors suffer from low energy density and the complexity of fabrication, which calls for their further development. In recent years, the fortification of graphene has enabled the dramatic improvement of planar micro-supercapacitors by taking full advantage of in-plane interdigital architecture and the unique features of graphene. The development of viable printing technologies has also provided better means for manufacturing, bringing micro-supercapacitors closer to practical applications. This review summarizes the latest advances in graphene-based planar micro-supercapacitors, with specific emphasis placed on formulation of graphene-based inks and their fabrication routes onto interdigital electrodes. Prospects and challenges in this field are also discussed towards the realization of graphene-based planar micro-supercapacitors in the world of microelectronics.
The Effect of Hemostatic Agents on the Retention Strength of Zirconia Crowns Luted to Dentin Abutments
Authors: Maischberger Christian , Liebermann Anja , Stawarczyk Bogna
Published: March 2019

The purpose of this study was to investigate whether hemostatic agents (HA) show an effect on the retention strength (RS) of zirconia crowns luted to dentin abutments after cleaning with an air/water spray. Human molars (N = 60/n = 12) were prepared and zirconia crowns were milled. Prior to luting (Scotchbond Universal/RelyX Ultimate), molars were pretreated using HA: i. 25% AlCl3 (VSC), ii. 20% Fe2(SO4)3 (VS), iii. 15.5% Fe2(SO4)3 (AS), iv. 12.7% iron ion solution (ASX) and v. no pretreatment (control). Mastication simulation and pull-out tests were performed. Failure types were defined: cohesive 1—tooth root; cohesive 2—tooth crown; adhesive 1—cement on tooth; adhesive 2—cement on tooth and crown; mixed—adhesive/cohesive. Data were analyzed using 1-way ANOVA, post-hoc Scheffé, Pearson’s chi-square-test and Ciba–Geigy table (p = 0.05). No RS differences between the tested groups were observed (p = 0.200). ASX fractured more cohesive 2 than the control group. VSC showed more cohesive 2 than adhesive 1 fractures. VS showed more adhesive 2 than mixed fractures. AS showed more cohesive 2 than adhesive 1 and more adhesive 2 than mixed fractures. ASX showed predominantly cohesive 2 fractures. RS was not affected when HA were cleaned off by 30 s of air/water spray prior to luting. HA still seem to weaken the dentin abutment, making it prone to cohesive fractures.
Influence of the Elastic Modulus on the Osseointegration of Dental Implants
Authors: Brizuela Aritza , Herrero-Climent Mariano , Rios-Carrasco Elisa , Rios-Santos Jose Vicente , Pérez Roman A. , Manero Jose Maria , Gil Mur Javier
Published: March 2019

The load transfer from metallic prosthesis to tissue plays an important role in the success of a designed device. From a mechanical behavior point of view, the load transfer will be favored when the elastic modulus between the metallic implant and the bone tissue are similar. Titanium and Ti-6Al-4V are the most commonly used metals and alloys in the field of dental implants, although they present high elastic moduli and hence trigger bone resorption. We propose the use of low-modulus β-type titanium alloys that can improve the growth of new bone surrounding the implant. We designed dental implants with identical morphology and micro-roughness composed of: Ti-15Zr, Ti-19.1Nb-8.8Zr, Ti-41.2Nb-6.1Zr, and Ti-25Hf-25Ta. The commercially pure Ti cp and Ti-6Al-4V were used as control samples. The alloys were initially mechanically characterized with a tensile test using a universal testing machine. The results showed the lowest elastic modulus for the Ti-25Hf-25Ta alloy. We implanted a total of six implants in the mandible (3) and maxilla (3) for each titanium alloy in six minipigs and evaluated their bone index contact (i.e., the percentage of new bone in contact with the metal—BIC%) after 3 and 6 weeks of implantation. The results showed higher BIC% for the dental implants with lowest elastic modulus, showing the importance of decreasing the elastic modulus of alloys for the successful osseointegration of dental implants.
Influence of W Addition on Microstructure and Mechanical Properties of Al-12%Si Alloys
Authors: Zykova Anna , Martyushev Nikita , Skeeba Vadim , Zadkov Denis , Kuzkin Andrey
Published: March 2019

A widespread method exerting the influence on the homogeneous formation of the microstructure and enhancement of strength properties of Al-Si alloys is a modification by super- and nanodispersed particles of different chemical compositions. In spite of the significant advances in the studies of the influence of various modifying compositions on the structure and mechanical properties of casted silumins, the literature contains no data about the influence of nanodispersed W-powder on formation of the structural-phase state and mechanical properties of Al-Si alloys. The paper considers the influence of 0.01–0.5 mass % W nanopowder on the structural-phase state and mechanical properties of an Al-12%Si alloy. It has been established that 0.1 mass % of W is an optimal addition. It results in the uniform distribution of eutectic (α-Al + Si), a 1.5-time decrease in the size of the plates of eutectic Si, a change of the shape of coarse plates (coarse plate-like or acicular) into a fine fibrous one, and an enhancement of the mechanical properties by 16–20%.
A Statistical Model of Cleavage Fracture Toughness of Ferritic Steel DIN 22NiMoCr37 at Different Temperatures
Authors: Qian Guian , Lei Wei-Sheng , Tong Zhenfeng , Yu Zhishui
Published: March 2019

It is a conventional practice to adopt Weibull statistics with a modulus of 4 for characterizing the statistical distribution of cleavage fracture toughness of ferritic steels, albeit based on a rather weak physical justification. In this study, a statistical model for cleavage fracture toughness of ferritic steels is proposed according to a new local approach model. The model suggests that there exists a unique correlation of the cumulative failure probability, fracture toughness and yield strength. This correlation is validated by the Euro fracture toughness dataset for 1CT specimens at four different temperatures, which deviates from the Weibull statistical model with a modulus of four.
Artificial Intelligence Approaches for Prediction of Compressive Strength of Geopolymer Concrete
Authors: Dao Dong Van , Ly Hai-Bang , Trinh Son Hoang , Le Tien-Thinh , Pham Binh Thai
Published: March 2019

Geopolymer concrete (GPC) has been used as a partial replacement of Portland cement concrete (PCC) in various construction applications. In this paper, two artificial intelligence approaches, namely adaptive neuro fuzzy inference (ANFIS) and artificial neural network (ANN), were used to predict the compressive strength of GPC, where coarse and fine waste steel slag were used as aggregates. The prepared mixtures contained fly ash, sodium hydroxide in solid state, sodium silicate solution, coarse and fine steel slag aggregates as well as water, in which four variables (fly ash, sodium hydroxide, sodium silicate solution, and water) were used as input parameters for modeling. A total number of 210 samples were prepared with target-specified compressive strength at standard age of 28 days of 25, 35, and 45 MPa. Such values were obtained and used as targets for the two AI prediction tools. Evaluation of the model’s performance was achieved via criteria such as mean absolute error (MAE), root mean square error (RMSE), and coefficient of determination (R2). The results showed that both ANN and ANFIS models have strong potential for predicting the compressive strength of GPC but ANFIS (MAE = 1.655 MPa, RMSE = 2.265 MPa, and R2 = 0.879) is better than ANN (MAE = 1.989 MPa, RMSE = 2.423 MPa, and R2 = 0.851). Sensitivity analysis was then carried out, and it was found that reducing one input parameter could only make a small change to the prediction performance.
Influence of PMMA on All-Inorganic Halide Perovskite CsPbBr3 Quantum Dots Combined with Polymer Matrix
Authors: Chen Lung-Chien , Tien Ching-Ho , Tseng Zong-Liang , Dong Yu-Shen , Yang Shengyi
Published: March 2019

The poor stability of CsPbX3 quantum dots (QDs-CsPbX3) under wet conditions is still considered to be a key issue. In order to overcome this problem, this study presents a high molecular weight polymer matrix (polymethylmethacrylate, PMMA) incorporated into the QDs-CsPbBr3 to improve its stability and maintain its excellent optical properties. In this study, the Cs2CO3, PbO, Tetrabutylammonium Bromide (TOAB) powder, oleic acid, and toluene solvent were uniformly mixed and purified to prepare high-quality QDs powders. Then, hexane was used as a dispersing agent for the QD powder to complete the perovskite QDs-CsPbBr3 solution. Finally, a solution with different proportions of quantum dots CsPbBr3 and PMMA was prepared and discussed. In the preparation of thin films, firstly, a thin film with the structure of glass/QD-CsPbBr3/PMMA was fabricated in a glove box using a well-developed QDs-CsPbBr3 solution by changing the ratio of CsPbBr3:PMMA. The material analysis of QDs-CsPbBr3 thin films was performed with photoluminescence (PL), transmittance, absorbance, and transmission electron microscopy (TEM). The structures and morphologies were further examined to study the effect of doped PMMA on perovskite QDs-CsPbBr3.
DHFR Inhibitors: Reading the Past for Discovering Novel Anticancer Agents
Authors: Raimondi Maria Valeria , Randazzo Ornella , La Franca Mery , Barone Giampaolo , Vignoni Elisa , Rossi Daniela , Collina Simona
Published: March 2019

Dihydrofolate reductase inhibitors are an important class of drugs, as evidenced by their use as antibacterial, antimalarial, antifungal, and anticancer agents. Progress in understanding the biochemical basis of mechanisms responsible for enzyme selectivity and antiproliferative effects has renewed the interest in antifolates for cancer chemotherapy and prompted the medicinal chemistry community to develop novel and selective human DHFR inhibitors, thus leading to a new generation of DHFR inhibitors. This work summarizes the mechanism of action, chemical, and anticancer profile of the DHFR inhibitors discovered in the last six years. New strategies in DHFR drug discovery are also provided, in order to thoroughly delineate the current landscape for medicinal chemists interested in furthering this study in the anticancer field.
Physalis peruviana-Derived 4β-Hydroxywithanolide E, a Novel Antagonist of Wnt Signaling, Inhibits Colorectal Cancer In Vitro and In Vivo
Authors: Ye Zhen-Nan , Yuan Feng , Liu Jie-Qing , Peng Xing-Rong , An Tao , Li Xue , Kong Ling-Mei , Qiu Ming-Hua , Li Yan
Published: March 2019

Deregulation of the Wnt signaling pathway leads to colorectal cancer progression. Natural dietary compounds serve as promising candidates for development as chemopreventive agents by suppressing the Wnt/β-catenin signaling pathway. Physalis peruviana-derived 4βHWE showed a significant inhibitory activity with a calculated IC50 of 0.09 μΜ in a screening of novel inhibitors of Wnt signaling with the dual-luciferase reporter assay. This study investigated the anti-tumor effect of 4βHWE and the potential Wnt signaling inhibitory mechanism. Both the western blot analysis and immunofluorescence assay showed that 4βHWE promoted the phosphorylation and degradation of β-catenin and the subsequent inhibition of its nuclear translocation to attenuate the endogenous Wnt target gene expression in colorectal cancer (CRC) cells. The cell viability assay indicated that 4βHWE preferentially inhibited the proliferation of CRC compared with CCD-841-CoN, a normal human colonic epithelial cell line. 4βHWE-mediated G0/G1 cell cycle arrest and apoptosis induction contributed to the suppression of the proliferation of CRC in the cell cycle and Annexin V-FITC/Propidium Iodide apoptosis analysis. Moreover, in vivo, 4βHWE dramatically inhibited tumor growth in HCT116 xenografts by attenuating the Wnt/β-catenin signaling pathway. In conclusion, our study suggested that 4βHWE could be of potential use in anti-tumor agent development as a novel Wnt signaling inhibitor.
Sulfide (Na2S) and Polysulfide (Na2S2) Interacting with Doxycycline Produce/Scavenge Superoxide and Hydroxyl Radicals and Induce/Inhibit DNA Cleavage
Authors: Misak Anton , Kurakova Lucia , Goffa Eduard , Brezova Vlasta , Grman Marian , Ondriasova Elena , Chovanec Miroslav , Ondrias Karol
Published: March 2019

Doxycycline (DOXY) is an antibiotic routinely prescribed in human and veterinary medicine for antibacterial treatment, but it has also numerous side effects that include oxidative stress, inflammation, cancer or hypoxia-induced injury. Endogenously produced hydrogen sulfide (H2S) and polysulfides affect similar biological processes, in which reactive oxygen species (ROS) play a role. Herein, we have studied the interaction of DOXY with H2S (Na2S) or polysulfides (Na2S2, Na2S3 and Na2S4) to gain insights into the biological effects of intermediates/products that they generate. To achieve this, UV-VIS, EPR spectroscopy and plasmid DNA (pDNA) cleavage assay were employed. Na2S or Na2S2 in a mixture with DOXY, depending on ratio, concentration and time, displayed bell-shape kinetics in terms of producing/scavenging superoxide and hydroxyl radicals and decomposing hydrogen peroxide. In contrast, the effects of individual compounds (except for Na2S2) were hardly observable. In addition, DOXY, as well as oxytetracycline and tetracycline, interacting with Na2S or other studied polysulfides reduced the •cPTIO radical. Tetracyclines induced pDNA cleavage in the presence of Na2S. Interestingly, they inhibited pDNA cleavage induced by other polysulfides. In conclusion, sulfide and polysulfides interacting with tetracyclines produce/scavenge free radicals, indicating a consequence for free radical biology under conditions of ROS production and tetracyclines administration.
The Pros and Cons of Cystic Fibrosis (CF) Patient Use of Herbal Supplements Containing Pulmonaria officinalis L. Extract: the Evidence from an In Vitro Study on Staphylococcus aureus CF Clinical Isolates
Authors: Sadowska Beata , Wójcik Urszula , Krzyżanowska-Kowalczyk Justyna , Kowalczyk Mariusz , Stochmal Anna , Rywaniak Joanna , Burzyńska Julia , Różalska Barbara
Published: March 2019

The justification for the use of herbal supplements with Pulmonaria officinalis L. extract (POE) in the case of staphylococcal lung colonization/infections characteristic for cystic fibrosis (CF), was examined in vitro. The impact of POE phenolic-rich fraction on the virulence attributes of CF-associated Staphylococcus aureus (S. aureus) clinical strains has been assessed, including pathogen adhesion, biofilm formation on native and protein-conditioned surfaces (mucin, elastin), mature biofilm eradication, staphylococcal protein A expression, α-toxin release, and S. a. adhesion to A549 cells. Cytotoxicity of the extract to lung epithelial cells was also investigated. It was found that POE has bacteriostatic effects at MIC 1–2 mg/mL, recognized as of limited efficacy, but at MIC/subMICs it targeted virulence not viability. It usually decreased S. aureus adhesion and less frequently inhibited biofilm formation on native and protein-conditioned surfaces. Observed effect seems to be related to significant reduction by POE of sortase A activity. However, in some cases POE favored the creation of biofilm by staphylococci and S. aureus adhesion to the lung epithelium was not limited. On the other side POE caused significant decrease of S. a. α-toxin synthesis and slightly weakened the expression of SpA. When used at supraMICs POE eradicated mature biofilm, but in some cases with unsatisfying outcomes. Promisingly, POE has been recognized as a safe product, with no cytotoxicity up to 4 mg/mL. These results reflect the positive, negative or neutral anti-staphylococcal properties of POE. It seems that POE may be beneficial as a prophylactic, but not as a therapeutic or supportive agent in the area of CF—integrative medicine. However, introduction the official recommendations needs further in vivo studies.
Anatolicin, a Highly Potent and Selective Cytotoxic Sesquiterpene Coumarin from the Root Extract of Heptaptera anatolica
Authors: Tosun Fatma , Beutler John A. , Ransom Tanya T. , Miski Mahmut
Published: March 2019

Seven known sesquiterpene coumarins and a new sesquiterpene coumarin, anatolicin (8), were isolated from the dichloromethane extract of the roots of Heptaptera anatolica. Structures of these compounds were elucidated based on their spectral properties. While some of these sesquiterpene coumarins showed modest cytotoxic activity against COLO205, KM12, A498, UO31, and TC32 cancer cell lines, selective cytotoxicity of anatolicin (8) and 14′-acetoxybadrakemin (7) were observed at nanomolar level against the UO31 kidney cancer cell line.
Natural Products and Obesity: A Focus on the Regulation of Mitotic Clonal Expansion during Adipogenesis
Authors: Chang Eugene , Kim Choon Young
Published: March 2019

Obesity is recognized as a worldwide health crisis. Obesity and its associated health complications such as diabetes, dyslipidemia, hypertension, and cardiovascular diseases impose a big social and economic burden. In an effort to identify safe, efficient, and long-term effective methods to treat obesity, various natural products with potential for inhibiting adipogenesis were revealed. This review aimed to discuss the molecular mechanisms underlying adipogenesis and the inhibitory effects of various phytochemicals, including those from natural sources, on the early stage of adipogenesis. We discuss key steps (proliferation and cell cycle) and their regulators (cell-cycle regulator, transcription factors, and intracellular signaling pathways) at the early stage of adipocyte differentiation as the mechanisms responsible for obesity.
Synthesis of AMPSA Polymeric Derivatives Monitored by Electrical Conductivity and Evaluation of Thermosensitive Properties of Resulting Microspheres
Authors: Gola Agnieszka , Sacharczuk Magdalena , Musiał Witold
Published: March 2019

Four stimuli-responsive polymers of N-isopropylacrylamide (NIPA) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) and crosslinked derivatives by N,N′-methylene bisacrylamide (MBA) were synthesized: PNA, PAMPSA, PNAM, PAMPSAM. The effect of the cross-linker and methyl sulphonic acid (-CH3-SO3H) group on particle size, aggregation and volume phase transition temperature (VPTT) was investigated. Polymeric particles were synthesized via the surfactant free precipitation polymerization (SFPP) at 70 °C in the presence of cationic initiator 2,2′-azobis[2-methylpropionamidine] dihydrochloride (AMP) system. Chemical composition and morphology of investigated samples were evaluated using ATR-FTIR spectroscopy, 1H-NMR spectrometry and SEM-EDS techniques. The hydrodynamic diameters (HD), zeta potential (ZP), and polydispersity index (PDI) in aqueous dispersions were assessed by dynamic light scattering (DLS) between 18–42 °C. HD values at 18 °C for PNA, PAMPSA, PNAM, PAMPSAM polymers were approx. 32, 730, 715, 665 nm, and ZP values were −1.36, −0.01, 8.90, −0.09 mV, respectively. The VPTT range was observed between 29 and 41 °C. PDI’s for PNA and PNAM were low and varied between 0.276 and 0.460, and between 0.119 and 0.056, respectively. PAMPSA and PAMPSAM were characterized by higher PDI in the range 0.728–0.959 and 0.658–0.836, respectively. The results confirmed the thermal sensitivity of the synthesized polymers and indicated a significant polydispersity and aggregation tendency of the resulting molecules. The conductivity results were applied for the interpretation of the polymerization process.
Density Functional Theory Investigations on the Mechanism of Formation of Pa(V) Ion in Hydrous Solutions
Authors: Ma Jun , Yang Chuting , Han Jun , Yu Jie , Hu Sheng , Yu Haizhu , Long Xinggui
Published: March 2019

Due to the enormous threat of protactinium to the environment and human health, its disposal and chemistry have long been important topics in nuclear science. [PaO(H2O)6]3+ is proposed as the predominant species in hydrous and acidic solutions, but little is known about its formation mechanism. In this study, density functional theory (DFT) calculations demonstrate a water coordination-proton transfer-water dissociation mechanism for the formation of PaO3+ in hydrous solutions. First, Pa(V) ion preferentially forms hydrated complexes with a coordination number of 10. Through hydrogen bonding, water molecules in the second coordination sphere easily capture two protons on the same coordinated H2O ligand to form [PaO(H2O)9]3+. Water dissociation then occurs to generate the final [PaO(H2O)6]3+, which is the thermodynamic product of Pa(V) in hydrous solutions.