bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship between Health-Related Quality of Life and Physical Activity in Children with Hyperactivity
Authors: Gallego-Méndez Julio , Perez-Gomez Jorge , Calzada-Rodríguez José Ignacio , Denche-Zamorano Ángel Manuel , Mendoza-Muñoz María , Carlos-Vivas Jorge , Garcia-Gordillo Miguel Ángel , Adsuar Jose C.
Published: April 2020

The main purpose of this paper was to evaluate the relationship between health-related quality of life (HRQoL) and the frequency of physical activity in Spanish children aged 8 to 14 years with attention deficit hyperactivity disorder (ADHD). Sample selection was performed using the data obtained from the children’s questionnaire of the National Health Survey of Spain 2017 that is carried out with the children’s parents, and that had an initial size of 6106 participants. After the application of the inclusion and exclusion criteria, the sample size was reduced to 496 subjects. Results show significant differences between the different levels of physical activity frequency, as well as a positive correlation of the average between HRQoL and ADHD. In conclusion, the practice of physical activity may contribute to the improvement of HRQoL in children with ADHD, possibly achieving greater benefits at higher levels of physical activity practice.
Field Study of the Interior Noise and Vibration of a Metro Vehicle Running on a Viaduct: A Case Study in Guangzhou
Authors: Yan Lei , Chen Zhou , Zou Yunfeng , He Xuhui , Cai Chenzhi , Yu Kehui , Zhu Xiaojie
Published: April 2020

The interior noise and vibration of metro vehicles have been the subject of increasing concern in recent years with the development of the urban metro systems. However, there still is a lack of experimental studies regarding the interior noise and vibration of metro vehicles. Therefore, overnight field experiments of the interior noise and vibration of a standard B-type metro train running on a viaduct were conducted on metro line 14 of Guangzhou (China). Both the A-weighted sound pressure level and linear sound pressure level were used to evaluate the interior noise signals in order to revel the underestimation of the low-frequency noise components. The results show that the interior noise concentrates in the low-to-middle frequency range. Increasing train speeds have significant effects on the sound pressure level inside the vehicle. However, two obvious frequency ranges (125–250 Hz and 400–1000 Hz) with respective corresponding center frequencies (160 Hz and 800 Hz) of the interior noise are nearly independent of train speed. The spectrum analysis of the vehicle body vibration shows that the frequency peak of the floor corresponds to the first frequency peak of the interior noise spectrum. There are two frequency peaks around 40 Hz and 160 Hz of the sidewall’s acceleration level. The frequency peaks of the acceleration level are also independent of the train speeds. It hopes that the field measurements in this paper can provide a data set for researchers for further investigations and can contribute to the countermeasures for reducing interior noise and vibration of a metro vehicle.
A Legal and Forensic Medicine Approach to Police Physical Intervention Techniques in High-Risk Situations
Authors: Vera-Jiménez José C. , Lorente José A. , González-Herrera Lucas , Álvarez José A. , Ferreiro-González Marta , Ayuso Jesús
Published: April 2020

Background: The physical intervention techniques (PITs) typically used by the police in troublesome situations are examined in terms of injuring potential depending on whether they target a body zone of high, medium or low vulnerability. Based on legal and forensic considerations, and principles of congruence, opportunity and proportionality, a need exists to favor opponent locking and arrest techniques targeting non-vulnerable zones to minimize the risk of severe damage. Methods: A search of the training manuals for the different kind of law of enforcement officers was carried out. Revision of injuries was available from electronic databases of academic o medical journals. Results: Three different locking and arrest PITs based on operational tactical procedures (OTP) that avoid zones of high or medium vulnerability are proposed. The new techniques use blocking, diverting and grabbing of the upper and lower limbs, followed by dislocation and locking of the same targets. Conclusions: The damaging potential of such PITs was assessed in terms of anatomical region and most were found to have a high risk of severe damage. The alternative PITs proposed here, which rely on OTP, improve in legal and forensic medical terms on existing choices and dramatically reduce the risk of injuring arrestees.
Sodium Intake and Target Organ Damage in Hypertension—An Update about the Role of a Real Villain
Authors: Nista Federica , Gatto Federico , Albertelli Manuela , Musso Natale
Published: April 2020

Salt intake is too high for safety nowadays. The main active ion in salt is sodium. The vast majority of scientific evidence points out the importance of sodium restriction for decreasing cardiovascular risk. International Guidelines recommend a large reduction in sodium consumption to help reduce blood pressure, organ damage, and cardiovascular risk. Regulatory authorities across the globe suggest a general restriction of sodium intake to prevent cardiovascular diseases. In spite of this seemingly unanimous consensus, some researchers claim to have evidence of the unhealthy effects of a reduction of sodium intake, and have data to support their claims. Evidence is against dissenting scientists, because prospective, observational, and basic research studies indicate that sodium is the real villain: actual sodium consumption around the globe is far higher than the safe range. Sodium intake is directly related to increased blood pressure, and independently to the enlargement of cardiac mass, with a possible independent role in inducing left ventricular hypertrophy. This may represent the basis of myocardial ischemia, congestive heart failure, and cardiac mortality. Although debated, a high sodium intake may induce initial renal damage and progression in both hypertensive and normotensive subjects. Conversely, there is general agreement about the adverse role of sodium in cerebrovascular disease. These factors point to the possible main role of sodium intake in target organ damage and cardiovascular events including mortality. This review will endeavor to outline the existing evidence.
Differences in Weekly Load Distribution Over Two Euroleague Seasons with a Different Head Coach
Authors: Salazar Hugo , Svilar Luka , Aldalur-Soto Ane , Castellano Julen
Published: April 2020

The weekly training management and competition loads are important aspects to optimize the performance of professional basketball players. The objectives of the study were (a) to describe the weekly external load (EL), as well as the internal response (IR), of elite basketball players over two consecutive seasons with a different head coach and (b) to compare weekly loads of different competitive densities. The data were collected from 27 elite players from the same team competing in the Spanish first division league (ACB) and EuroLeague during 2017–2018 and 2018–2019 seasons. EL was measured using microsensor technology to determine PlayerLoad values, expressed in arbitrary units (AU). Session rating of perceived exertion (sRPE) was used for IR quantification. Comparisons between the two seasons and of weeks with different competitive densities were made. The inter-week load variability was moderate-high for both seasons. The highest EL values were measured during the weeks with three games (W3) (W3 > W0 > W2 > W1), while the most demanding week for players’ IR was observed during weeks with no competition (W0). Additionally, higher EL (d = 0.31) and IR (d = 0.37) values were observed in season 2018–2019 compared to 2017–2018. The results obtained in this study contributed new data on the internal and external load required by professional basketball players in weeks with different number of games and showed that different coaching strategies may demand a different external and internal workload in consecutive seasons. Furthermore, the results highlighted the need to carry out an adequate load management program.
Assessing the Impact of Housing Features and Environmental Factors on Home Indoor Radon Concentration Levels on the Navajo Nation
Authors: Yazzie Sheldwin A. , Davis Scott , Seixas Noah , Yost Michael G.
Published: April 2020

Uranium is naturally found in the environment as a radioactive metal element with high concentrations in the Southwestern US. In this region is the Navajo Nation, which spans approximately 69,930 square kilometers. A decay product of uranium is radon gas, a lung carcinogen that has no color, odor, or taste. Radon gas may pass from soil into homes; and, indoor accumulation has been associated with geographical location, seasonality, home construction materials, and home ventilation. A home and indoor radon survey was conducted from November 2014 through May 2015, with volunteers who reported residence on the Navajo Nation. Home geolocation, structural characteristics, temperature (°C) during radon testing, and elevation (meters) were recorded. Short-term indoor radon kits were used to measure indoor radon levels. 51 homes were measured for indoor radon levels, with an arithmetic mean concentration of 60.5 Becquerels per cubic meter (Bq/m3) (SD = 42.7). The mean indoor radon concentrations (Bq/m3) by house type were: mobile, 29.0 (SD = 22.9); wood, 58.6 (SD = 36.0); hogan, 74.0 (SD = 0.0); homes constructed of cement and wood, 82.6 (SD = 3.5); and homes constructed of concrete and cement, 105.7 (SD = 55.8). A key observation is that house construction type appears to be associated with the mean home indoor radon concentration. This observation has been published in that the basic structural make-up of the home may affect home ventilation and therefore indoor radon concentration levels.
Density of Dermacentor reticulatus Ticks in Eastern Poland
Authors: Zając Zbigniew , Woźniak Aneta , Kulisz Joanna
Published: April 2020

Dermacentor reticulatus, the ornate cow tick, is second only to Ixodes ricinus as the most important reservoir and vector of infectious diseases in Europe. In recent years, the distribution of D. reticulatus ticks has expanded into new territories, including increased population densities in areas of their previous occurrence. Our investigations around this consisted of two stages. In the first stage, we monitored the seasonal activity of D. reticulatus ticks in Polesie National Park in 2014–2019. The second stage, which was carried out in 2019 at the peak of the spring (March) and autumn (October) activity of this species, included assessment of the density of D. reticulatus ticks in the entire province. To this end, the study area was divided into 101 equal plots that were surveyed for ticks. The seasonal activity of D. reticulatus in Polesie National Park showed peaks of activity in autumn in 2014–2018 and in spring in 2019. A total of 19,559 adult D. reticulatus specimens were collected, with a mean of 96.8 specimens/100 m2 in Lublin Province. The area of Lublin Province is characterized by a high density of the ornate cow tick. An increase in the surface area of meadows and fallow land has contributed to a rise in the number of local populations of D. reticulatus ticks.
Providing Access to Urban Green Spaces: A Participatory Benefit-Cost Analysis in Spain
Authors: García de Jalón Silvestre , Chiabai Aline , Mc Tague Alyvia , Artaza Naiara , de Ayala Amaia , Quiroga Sonia , Kruize Hanneke , Suárez Cristina , Bell Ruth , Taylor Timothy
Published: April 2020

The opening up of green spaces could provide significant benefits to society. This study develops a framework to assess the economic benefits and costs of public interventions providing citizen access to urban green spaces. The Thinking Fadura project in Getxo (Spain) was used as a case study. A method for participatory benefit-cost analysis is developed, where a stakeholder-participatory evaluation is combined with a standard cost-benefit analysis. The participatory evaluation followed a bottom-up approach in a sequential evaluation including three main focal points: key stakeholders and experts, visitors and the general public. The assessment demonstrates that the Thinking Fadura project’s benefits outweigh the costs. The results suggest that projects designed with the purpose of improving green space accessibility to the general public can be beneficial from a societal perspective. The highest economic benefits were an increase in the amenity and recreational value and an increase in people’s physical activity. The participatory evaluation indicates that giving access to people of lower socio-economic status and vulnerable groups and improving recreational use were perceived as the most beneficial. An increase in noise, dirt, and risk of criminal activities as well as potential conflicts between green space users were perceived as the most negative impacts of opening a previously restricted area to the general public. The economic assessment of Thinking Fadura project could serve as a model in the decision-making process in locations where the use of greenspaces is restricted.
Environmental Regulation, Trade Comparative Advantage, and the Manufacturing Industry’s Green Transformation and Upgrading
Authors: Gong Mengqi , You Zhe , Wang Linting , Cheng Jinhua
Published: April 2020

This paper is the first to systematically review the theoretical mechanisms of environmental regulation and trade comparative advantage that affect the green transformation and upgrading of the manufacturing industry. On this basis, corresponding hypotheses are put forward. The non-radial and non-angle SBM (slacks-based measure) efficiency measurement model with undesirable outputs was used, combined with the use of the ML (green total factor productivity index) productivity index to measure green total factor productivity. Finally, the theoretical hypothesis was empirically tested using data from 27 manufacturing industries in China from 2005 to 2017. The results show the following: (1) There is a significant inverted U-shaped curve relationship between environmental regulation and the transformation of the manufacturing industry. In other words, as environmental regulation increases, its impact on the transformation and upgrading of the manufacturing industry is first promoted and then suppressed. (2) When there are no environmental regulations, the trade comparative advantage of the manufacturing industry is not conducive to industrial transformation. However, under the constraints of environmental regulations, the comparative advantage of trade will significantly promote the green transformation and upgrading of manufacturing. Therefore, in order to effectively promote transformation and upgrading of the manufacturing, this paper proposes the following policy recommendations: (1) The Chinese government should pay more attention to the impact of environmental regulation intensity on the transformation of manufacturing industries, further increase the intensity of environmental regulation within the reasonable range, and fully exert the positive effects of environmental regulation on the trade patterns and manufacturing industry transformation. (2) We should further optimize the structure of trade, realize the diversification of manufacturing import and export, and promote its transformation into high-end manufacturing. On this basis, green production technology in the manufacturing industry can be improved through the technology spillover effect. (3) Efforts should be made to improve the level of collaborative development between environmental regulation and trade patterns and to explore the transformation path of the manufacturing industry with the integration of environmental regulation and trade patterns.
Sense of Coherence, Compassionate Love and Coping in International Leaders during the Transition into the Fourth Industrial Revolution
Authors: Mayer Claude-Hélène , Oosthuizen Rudolf M.
Published: April 2020

Contemporary workplaces are influenced by rapid changes, high levels of competition, increasing complexities and internationalisation processes. At the edge of the Fourth Industrial Revolution (4IR), insecurities and anxieties are high, and leaders are encouraged more than ever to lead employees with meaningful vision and prudence in order to make use of employees’ strengths, and ensure mental health and well-being. The aim of this article is to present new insights into salutogenesis, particularly sense of coherence (SOC), compassionate love (CL), and coping (C) in leaders with different cultural backgrounds. This study strengthens the idea that CL is a coping mechanism. This coping mechanism can be used by leaders to establish a resilient and salutogenic organisations. This article explores the subjective perspectives of 22 international leaders from five different countries and their views regarding SOC, CL and C through a qualitative research approach, using a qualitative online questionnaire for data collection and content analysis for data analysis. The findings on the perspectives of leaders provide new and original insights into how SOC, CL and C are connected, and how these concepts contribute to healthy organisations which are on their way to the transition into the 4IR. Conclusions are drawn. Recommendations for future research and practice are given.
Assessing Chromium Contamination in Red Soil: Monitoring the Migration of Fractions and the Change of Related Microorganisms
Authors: Zhang Siyuan , Hao Xiaodong , Tang Jiahui , Hu Jin , Deng Yan , Xu Menglong , Zhu Ping , Tao Jiemeng , Liang Yili , Yin Huaqun , Jiang Luhua , Liu Xueduan , Liu Hongwei
Published: April 2020

The improper stacking of chromium (Cr) slag poses a great threat to the environment and human health. The toxicity of Cr in soil is not only related to its total amount, but also to its fractions. A simulated experiment was conducted in laboratory to assess the environmental risk of Cr fractions migration and distribution in red soil. The results showed the content of acid-soluble and reducible Cr significantly decreased (P < 0.05) in top layer but increased in middle and substratum layers over time. This indicated that acid-soluble and reducible Cr migrated downward with time and the relative mobility of acid-soluble Cr (0.038 mg/kg·d·m) was higher than that of reducible Cr (0.028 mg/kg·d·m). Furthermore, correlation analysis between microbial community and chromium fraction showed the relative abundance of Lysobacter, Flavihumibacter, Flavisolbacter, and Altererythrobacter was significantly (P < 0.05) correlated with acid-soluble and reducible fractions. Thus, these microorganisms might be evaluators to assess the migration of acid-soluble and reducible fractions in red soil. In summary, this study provided a new comprehension on remediation of Cr-contaminated soil by monitoring the migration of acid-soluble and reducible fractions and the changes of related microbial groups.
Association between Post-Diagnosis Particulate Matter Exposure among 5-Year Cancer Survivors and Cardiovascular Disease Risk in Three Metropolitan Areas from South Korea
Authors: Choi Seulggie , Kim Kyae Hyung , Kim Kyuwoong , Chang Jooyoung , Kim Sung Min , Kim Seong Rae , Cho Yoosun , Lee Gyeongsil , Son Joung Sik , Park Sang Min
Published: April 2020

Cancer survivors are at an increased risk for cardiovascular disease (CVD). However, the association between particulate matter (PM) and CVD risk among cancer survivors (alive >5 years since diagnosis) is unclear. We investigated the risk of CVD among 40,899 cancer survivors within the Korean National Health Insurance Service database. Exposure to PM was determined by assessing yearly average PM levels obtained from the Air Korea database from 2008 to 2011. PMs with sizes <2.5 (PM2.5), <10 (PM10), or 2.5–10 (PM2.5–10) μm in diameter were compared, with each PM level exposure further divided into quintiles. Patients were followed up from January 2012 to date of CVD event, death, or December 2017, whichever came earliest. Adjusted hazard ratios (aHRs) and 95% confidence intervals (CIs) for CVD were calculated using Cox proportional hazards regression by PM exposure levels. Compared with cancer survivors in the lowest quintile of PM2.5 exposure, those within the highest quintile had a greater risk for CVD (aHR 1.31, 95% CI 1.07–1.59). Conversely, increasing PM10 and PM2.5–10 levels were not associated with increased CVD risk (p for trend 0.078 and 0.361, respectively). Cancer survivors who reduce PM2.5 exposure may benefit from lower risk of developing CVD.
Patient Assessment and Chronic Pain Self-Management in Ethnomedicine: Seasonal and Ecosystemic Embodiment in Ayurvedic Patient-Centered Care
Authors: Agarwal Vinita
Published: April 2020

Background: Ayurveda’s preventive focus complements its strength with the interventionist approach of the biomedical in chronic pain self-management. Patient-centered care (PCC) using ethnomedicine promises greater patient self-management; however, few studies have examined environmental relationships and PCC in self-management of chronic pain through Ayurveda. Objective: To examine how Ayurveda’s philosophical focus on whole system frameworks describes the integration of the individual and the ecological in tailoring an integrative patient-centered diagnostic and prognostic approach to chronic pain management. Methods: This qualitative case study conducted in-depth semi-structured interviews of Ayurvedic physicians from India (N = 10) and a qualitative inductive content analytic approach. Findings: The diagnostic and interpretational framework of the doshas supports the integration of the individual and the ecological through (a) the circadian and seasonal cycles relating mind-body awareness with diet, lifestyle (e.g., yoga), and breath (e.g., pranayama), and (b) biogeographical and ecosystemic regions relating the biogeographical and the ecological (e.g., desh) with the regulatory principle of pain and its physiological and anatomical perception (vata) in an approach that goes beyond treating pain etiology to a whole person PCC approach. Conclusions: The study highlights how circadian and seasonal cycles and evolutionary spatial-temporal factors of biogeographical and ecological regions are employed in patient assessment and self-management to support patient involvement. Recommendations for PCC in integrative chronic pain management include supporting patient ownership of their care through the dosha framework that relates the individual and the ecological in the patient’s own life-context and supports co-creation of a collaborative plan of care using an ethnomedical framework.
Data Envelopment Analysis and Multifactor Asset Pricing Models
Authors: Solórzano-Taborga Pablo , Alonso-Conde Ana Belén , Rojo-Suárez Javier
Published: April 2020

Recent literature shows that market anomalies have significantly diminished, while research on market factors has largely improved the performance of asset pricing models. In this paper we study the extent to which data envelopment analysis (DEA) techniques can help improve the performance of multifactor models. Specifically, we test the explanatory power of the Fama and French three-factor model, combined with an additional factor based on DEA, on a sample of 2101 European equity funds, for the period from 2001 to 2016. Accordingly, we first form the fund portfolios that constitute our test assets and create the efficiency factor. Secondly, we estimate the prices of risk tied to the four factors using ordinary least squares (OLS) on a two-stage cross-sectional regression. Finally, we use the R-squared statistic estimated by generalized least squares (GLS), as well as the Gibbons Ross and Shanken test and the J-test for overidentifying restrictions in order to study the performance of the model, including and omitting the efficiency factor. The results show that the efficiency factor improves the performance of the model and reduces the pricing errors of the assets under consideration, which allows us to conclude that the efficiency index may be used as a factor in asset pricing models.
Stock Market Contagion during the Global Financial Crises: Evidence from the Chilean Stock Market
Authors: Mahenthiran Sakthi , Gjerde Tom , Silva Berta
Published: April 2020

The study examines evidence for the transmission of the US and EU financial crises via investor holdings into the Chilean stock market following two global financial crises, in 2008 and 2011. The study modified the models of Bekaert et al. (2014), and Dungey and Gajurel (2015) on the 2007–2009 global financial crisis and extends the period to include the European debt crisis of 2010–2011. The study produced three main contributions. First, changes in the equity holdings of retail investors were a key source of contagion following the 2008 US financial crisis. Second, investor herding during the 2011 financial crisis is shown to be low based on the co-movement of equity holdings between the four investor groups studied. Third, investor behavior during the 2011 EU crisis differs from that of the 2008 US financial crisis, which we attribute to firms in Chile adopting international financial reporting standards (IFRS) and improving their corporate governance. We compared the findings to the prior contagion studies that rely on Chilean return data to highlight the contributions to international financial research, particularly as it relates to the functioning of emerging capital markets during financial crises.
Determination of the Normalized Difference Vegetation Index (NDVI) with Top-of-Canopy (TOC) Reflectance from a KOMPSAT-3A Image Using Orfeo ToolBox (OTB) Extension
Authors: Lee Kiwon , Kim Kwangseob , Lee Sun-Gu , Kim Yongseung
Published: April 2020

Surface reflectance data obtained by the absolute atmospheric correction of satellite images are useful for land use applications. For Landsat and Sentinel-2 images, many radiometric processing methods exist, and the images are supported by most types of commercial and open-source software. However, multispectral KOMPSAT-3A images with a resolution of 2.2 m are currently lacking tools or open-source resources for obtaining top-of-canopy (TOC) reflectance data. In this study, an atmospheric correction module for KOMPSAT-3A images was newly implemented into the optical calibration algorithm in the Orfeo Toolbox (OTB), with a sensor model and spectral response data for KOMPSAT-3A. Using this module, named OTB extension for KOMPSAT-3A, experiments on the normalized difference vegetation index (NDVI) were conducted based on TOC reflectance data with or without aerosol properties from AERONET. The NDVI results for these atmospherically corrected data were compared with those from the dark object subtraction (DOS) scheme, a relative atmospheric correction method. The NDVI results obtained using TOC reflectance with or without the AERONET data were considerably different from the results obtained from the DOS scheme and the Landsat-8 surface reflectance of the Google Earth Engine (GEE). It was found that the utilization of the aerosol parameter of the AERONET data affects the NDVI results for KOMPSAT-3A images. The TOC reflectance of high-resolution satellite imagery ensures further precise analysis and the detailed interpretation of urban forestry or complex vegetation features.
An Efficient Indoor Wi-Fi Positioning Method Using Virtual Location of AP
Authors: Xu Fan , Hu Xuke , Luo Shuaiwei , Shang Jianga
Published: April 2020

Wi-Fi fingerprinting has been widely used for indoor localization because of its good cost-effectiveness. However, it suffers from relatively low localization accuracy and robustness owing to the signal fluctuations. Virtual Access Points (VAP) can effectively reduce the impact of signal fluctuation problem in Wi-Fi fingerprinting. Current techniques normally use the Log-Normal Shadowing Model to estimate the virtual location of the access point. This would lead to inaccurate location estimation due to the signal attenuation factor in the model, which is difficult to be determined. To overcome this challenge, in this study, we propose a novel approach to calculating the virtual location of the access points by using the Apollonius Circle theory, specifically the distance ratio, which can eliminate the attenuation parameter term in the original model. This is based on the assumption that neighboring locations share the same attenuation parameter corresponding to the signal attenuation caused by obstacles. We evaluated the proposed method in a laboratory building with three different kinds of scenes and 1194 test points in total. The experimental results show that the proposed approach can improve the accuracy and robustness of the Wi-Fi fingerprinting techniques and achieve state-of-art performance.
Deep Learning for Fingerprint Localization in Indoor and Outdoor Environments
Authors: Li Da , Lei Yingke , Li Xin , Zhang Haichuan
Published: April 2020

Wi-Fi and magnetic field fingerprinting-based localization have gained increased attention owing to their satisfactory accuracy and global availability. The common signal-based fingerprint localization deteriorates due to well-known signal fluctuations. In this paper, we proposed a Wi-Fi and magnetic field-based localization system based on deep learning. Owing to the low discernibility of magnetic field strength (MFS) in large areas, the unsupervised learning density peak clustering algorithm based on the comparison distance (CDPC) algorithm is first used to pick up several center points of MFS as the geotagged features to assist localization. Considering the state-of-the-art application of deep learning in image classification, we design a location fingerprint image using Wi-Fi and magnetic field fingerprints for localization. Localization is casted in a proposed deep residual network (Resnet) that is capable of learning key features from a massive fingerprint image database. To further enhance localization accuracy, by leveraging the prior information of the pre-trained Resnet coarse localizer, an MLP-based transfer learning fine localizer is introduced to fine-tune the coarse localizer. Additionally, we dynamically adjusted the learning rate (LR) and adopted several data enhancement methods to increase the robustness of our localization system. Experimental results show that the proposed system leads to satisfactory localization performance both in indoor and outdoor environments.
Application of Hybrid Prediction Methods in Spatial Assessment of Inland Excess Water Hazard
Authors: Laborczi Annamária , Bozán Csaba , Körösparti János , Szatmári Gábor , Kajári Balázs , Túri Norbert , Kerezsi György , Pásztor László
Published: April 2020

Inland excess water is temporary water inundation that occurs in flat-lands due to both precipitation and groundwater emerging on the surface as substantial sources. Inland excess water is an interrelated natural and human induced land degradation phenomenon, which causes several problems in the flat-land regions of Hungary covering nearly half of the country. Identification of areas with high risk requires spatial modelling, that is mapping of the specific natural hazard. Various external environmental factors determine the behavior of the occurrence, frequency of inland excess water. Spatial auxiliary information representing inland excess water forming environmental factors were taken into account to support the spatial inference of the locally experienced inland excess water frequency observations. Two hybrid spatial prediction approaches were tested to construct reliable maps, namely Regression Kriging (RK) and Random Forest with Ordinary Kriging (RFK) using spatially exhaustive auxiliary data on soil, geology, topography, land use, and climate. Comparing the results of the two approaches, we did not find significant differences in their accuracy. Although both methods are appropriate for predicting inland excess water hazard, we suggest the usage of RFK, since (i) it is more suitable for revealing non-linear and more complex relations than RK, (ii) it requires less presupposition on and preprocessing of the applied data, (iii) and keeps the range of the reference data, while RK tends more heavily to smooth the estimations, while (iv) it provides a variable rank, providing explicit information on the importance of the used predictors.
Genome-Wide Identification and Characterization of Drought Stress Responsive microRNAs in Tibetan Wild Barley
Authors: Qiu Cheng-Wei , Liu Li , Feng Xue , Hao Peng-Fei , He Xiaoyan , Cao Fangbin , Wu Feibo
Published: April 2020

Drought stress is a major obstacle to agricultural production. Tibetan wild barley with rich genetic diversity is useful for drought-tolerant improvement of cereals. MicroRNAs (miRNAs) play critical roles in controlling gene expression in response to various environment perturbations in plants. However, the genome-wide expression profiles of miRNAs and their targets in response to drought stress are largely unknown in wild barley. In this study, a polyethylene glycol (PEG) induced drought stress hydroponic experiment was performed, and the expression profiles of miRNAs from the roots of two contrasting Tibetan wild barley genotypes XZ5 (drought-tolerant) and XZ54 (drought-sensitive), and one cultivated barley Tadmor (drought-tolerant) generated by high-throughput sequencing were compared. There were 69 conserved miRNAs and 1574 novel miRNAs in the dataset of three genotypes under control and drought conditions. Among them, seven conserved miRNAs and 36 novel miRNAs showed significantly genotype-specific expression patterns in response to drought stress. And 12 miRNAs were further regarded as drought tolerant associated miRNAs in XZ5, which mostly participate in gene expression, metabolism, signaling and transportation, suggesting that they and their target genes play important roles in plant drought tolerance. This is the first comparation study on the miRNA transcriptome in the roots of two Tibetan wild barley genotypes differing in drought tolerance and one drought tolerant cultivar in response to PEG treatment. Further results revealed the candidate drought tolerant miRNAs and target genes in the miRNA regulation mechanism in wild barley under drought stress. Our findings provide valuable understandings for the functional characterization of miRNAs in drought tolerance.
Transcriptional and Metabolic Changes Associated with Phytoglobin Expression during Germination of Barley Seeds
Authors: Zafari Somaieh , Hebelstrup Kim H. , Igamberdiev Abir U.
Published: April 2020

To understand how the class 1 phytoglobin is involved in germination process via the modulation of the nitric oxide (NO) metabolism, we performed the analysis of physiological and molecular parameters in the embryos of transgenic barley (Hordeum vulgare L. cv Golden Promise) plants differing in expression levels of the phytoglobin (Pgb1) gene during the first 48 h of germination. Overexpression of Pgb1 resulted in a higher rate of germination, higher protein content and higher ATP/ADP ratios. This was accompanied by a lower rate of NO emission after radicle protrusion, as compared to the wild type and downregulating line, and a lower rate of S-nitrosylation of proteins in the first hours postimbibition. The rate of fermentation estimated by the expression and activity of alcohol dehydrogenase was significantly higher in the Pgb1 downregulating line, the same tendency was observed for nitrate reductase expression. The genes encoding succinate dehydrogenase and pyruvate dehydrogenase complex subunits were more actively expressed in embryos of the seeds overexpressing Pgb1. It is concluded that Pgb1 expression in embryo is essential for the maintenance of redox and energy balance before radicle protrusion, when seeds experience low internal oxygen concentration and exerts the effect on metabolism during the initial development of seedlings.
Higher Accumulation of Docosahexaenoic Acid in the Vermilion of the Human Lip than in the Skin
Authors: Mamun Md. Al , Sato Shumpei , Naru Eiji , Sakata Osamu , Hoshikawa Emi , Suzuki Ayako , Islam Ariful , Kahyo Tomoaki , Sato Tomohito , Ito Takashi K. , Horikawa Makoto , Fukui Reimu , Izumi Kenji , Setou Mitsutoshi
Published: April 2020

The vermilion of the human lip is a unique facial area because of certain distinguishing features from the adjacent tissues such as the white lip (skin) and oral mucosa. However, the distinction in terms of molecular distribution between the vermilion and skin has remained unexplored. Therefore, we aimed to map the human lip by mass spectrometry imaging to gain understanding of the free fatty acid distribution in the vermilion. The lip specimens trimmed off during cheiloplasty were analyzed using desorption electrospray ionization–mass spectrometry imaging. Distributions of two monounsaturated fatty acids and three polyunsaturated fatty acids were observed in the human lip tissue: palmitoleic acid (POA) and oleic acid (OA) and linoleic acid (LA), arachidonic acid (AA), and docosahexaenoic acid (DHA), respectively. Although POA, OA, LA, and AA were differentially distributed across the vermilion and skin, DHA showed a higher accumulation in the epithelium of the vermilion compared to that in the skin. Our results clearly demonstrated the difference in fatty acid distributions between the vermilion and skin. The highly abundant DHA in the epithelium of the vermilion may have an antioxidant role and may thus protect the lip from aging. Our findings can provide a novel strategy for treating lip disorders.
Application of Solid-State Nanopore in Protein Detection
Authors: Luo Yuhan , Wu Linlin , Tu Jing , Lu Zuhong
Published: April 2020

A protein is a kind of major biomacromolecule of life. Its sequence, structure, and content in organisms contains quite important information for normal or pathological physiological process. However, research of proteomics is facing certain obstacles. Only a few technologies are available for protein analysis, and their application is limited by chemical modification or the need for a large amount of sample. Solid-state nanopore overcomes some shortcomings of the existing technology, and has the ability to detect proteins at a single-molecule level, with its high sensitivity and robustness of device. Many works on detection of protein molecules and discriminating structure have been carried out in recent years. Single-molecule protein sequencing techniques based on solid-state nanopore are also been proposed and developed. Here, we categorize and describe these efforts and progress, as well as discuss their advantages and drawbacks.
Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status—A Pilot Study
Authors: Han Pingping , Bartold Peter Mark , Salomon Carlos , Ivanovski Saso
Published: April 2020

This pilot study aims to investigate whether salivary small extracellular vesicle (sEV)-associated microRNAs could act as potential biomarkers for periodontal disease status. Twenty-nine participants (10 who were healthy, nine with gingivitis, 10 with stage III/IV periodontitis) were recruited and unstimulated whole saliva samples were collected. Salivary sEVs were isolated using the size-exclusion chromatography (SEC) method and characterised by morphology, EV-protein and size distribution using transmission electron microscopy (TEM), Western Blot and Nanoparticle Tracking Analysis (NTA), respectively. Ten mature microRNAs (miRNAs) in salivary sEVs and saliva were evaluated using RT-qPCR. The discriminatory power of miRNAs as biomarkers in gingivitis and periodontitis versus healthy controls was evaluated by Receiver Operating Characteristics (ROC) curves. Salivary sEVs were comparable to sEVs morphology, mode, size distribution and particle concentration in healthy, gingivitis and periodontitis patients. Compared to miRNAs in whole saliva, three significantly increased miRNAs (hsa-miR-140-5p, hsa-miR-146a-5p and hsa-miR-628-5p) were only detected in sEVs in periodontitis when compared to that of healthy controls, with a good discriminatory power (area under the curve (AUC) = 0.96) for periodontitis diagnosis. Our study demonstrated that salivary sEVs are a non-invasive source of miRNAs for periodontitis diagnosis. Three miRNAs that are selectively enriched in sEVs, but not whole saliva, could be potential biomarkers for periodontal disease status.
Monoamine Oxidase B Expression Correlates with a Poor Prognosis in Colorectal Cancer Patients and Is Significantly Associated with Epithelial-to-Mesenchymal Transition-Related Gene Signatures
Authors: Yang Yi-Chieh , Chien Ming-Hsien , Lai Tsung-Ching , Su Chia-Yi , Jan Yi-Hua , Hsiao Michael , Chen Chi-Long
Published: April 2020

Monoamine oxidases (MAOs) including MAOA and MAOB are enzymes located on the outer membranes of mitochondria, which are responsible for catalyzing monoamine oxidation. Recently, increased level of MAOs were shown in several cancer types. However, possible roles of MAOs have not yet been elucidated in the progression and prognosis of colorectal carcinoma (CRC). We therefore analyzed the importance of MAOs in CRC by an in silico analysis and tissue microarrays. Several independent cohorts indicated that high expression of MAOB, but not MAOA, was correlated with a worse disease stage and poorer survival. In total, 203 colorectal adenocarcinoma cases underwent immunohistochemical staining of MAOs, and associations with clinicopathological parameters and patient outcomes were evaluated. We found that MAOB is highly expressed in CRC tissues compared to normal colorectal tissues, and its expression was significantly correlated with a higher recurrence rate and a poor prognosis. Moreover, according to the univariate and multivariate analyses, we found that MAOB could be an independent prognostic factor for overall survival and disease-free survival, and its prognostic value was better than T and N stage. Furthermore, significant positive and negative correlations of MAOB with mesenchymal-type and epithelial-type gene expressions were observed in CRC tissues. According to the highlighted characteristics of MAOB in CRC, MAOB can be used as a novel indicator to predict the progression and prognosis of CRC patients.
Insights into the Regulatory Role of m6A Epitranscriptome in Glioblastoma
Authors: Galardi Silvia , Michienzi Alessandro , Ciafrè Silvia Anna
Published: April 2020

N6-methyladenosine (m6A) is one of the most widespread and abundant internal messenger RNA modifications found in eukaryotes. Emerging evidence suggests that this modification is strongly linked to the activation and inhibition of cancer pathways and is associated with prognostically significant tumour subtypes. The present review describes the dynamic nature of m6A regulator enzymes, as methyltransferases, demethylases and m6A binding proteins, and points out thevalue of the balance among these proteins in regulating gene expression, cell metabolism and cancer development. The main focus of this review is on the roles of m6A modification in glioblastoma, the most aggressive and invariably lethal brain tumour. Although the study of m6A in glioblastoma is a young one, and papers in this field can yield divergent conclusions, the results collected so far clearly demonstrate that modulation of mRNA m6A levels impacts multiple aspects of this tumour, including growth, glioma stem cells self-renewal, and tumorigenesis, suggesting that mRNA m6A modification may serve as a promising target for glioblastoma therapy. We also present recent data about another type of epitranscriptomic modification, the methylation of cytosine at a specific site of 28S rRNA, as it was recently shown to affect the biology of glioma cells, with high potential of clinical implications.
Regeneration of Pulmonary Tissue in a Calf Model of Fibrinonecrotic Bronchopneumonia Induced by Experimental Infection with Chlamydia psittaci
Authors: Liebler-Tenorio Elisabeth M. , Lambertz Jacqueline , Ostermann Carola , Sachse Konrad , Reinhold Petra
Published: April 2020

Pneumonia is a cause of high morbidity and mortality in humans. Animal models are indispensable to investigate the complex cellular interactions during lung injury and repair in vivo. The time sequence of lesion development and regeneration is described after endobronchial inoculation of calves with Chlamydia psittaci. Calves were necropsied 2–37 days after inoculation (dpi). Lesions and presence of Chlamydia psittaci were investigated using histology and immunohistochemistry. Calves developed bronchopneumonia at the sites of inoculation. Initially, Chlamydia psittaci replicated in type 1 alveolar epithelial cells followed by an influx of neutrophils, vascular leakage, fibrinous exudation, thrombosis and lobular pulmonary necrosis. Lesions were most extensive at 4 dpi. Beginning at 7 dpi, the number of chlamydial inclusions declined and proliferation of cuboidal alveolar epithelial cells and sprouting of capillaries were seen at the periphery of necrotic tissue. At 14 dpi, most of the necrosis had been replaced with alveoli lined with cuboidal epithelial cells resembling type 2 alveolar epithelial cells and mild fibrosis, and hyperplasia of organized lymphoid tissue were observed. At 37 dpi, regeneration of pulmonary tissue was nearly complete and only small foci of remodeling remained. The well-defined time course of development and regeneration of necrotizing pneumonia allows correlation of morphological findings with clinical data or treatment regimen.
The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
Authors: Kim Si Won , Lee Jung Seok , Park Seong Bin , Lee Ae Rin , Jung Jae Wook , Chun Jin Hong , Lazarte Jassy Mary S. , Kim Jaesung , Seo Jong-Su , Kim Jong-Hwan , Song Jong-Wook , Ha Min Woo , Thompson Kim D. , Lee Chang-Ro , Jung Myunghwan , Jung Tae Sung
Published: April 2020

Gram-negative bacteria have an outer membrane inhibiting the entry of antibiotics. Porins, found within the outer membrane, are involved in regulating the permeability of β-lactam antibiotics. β-lactamases are enzymes that are able to inactivate the antibacterial properties of β-lactam antibiotics. Interestingly, porins and β-lactamase are found in outer membrane vesicles (OMVs) of β-lactam-resistant Escherichia coli and may be involved in the survival of susceptible strains of E. coli in the presence of antibiotics, through the hydrolysis of the β-lactam antibiotic. In this study, OMVs isolated from β-lactam-resistant E. coli and from mutants, lacking porin or β-lactamase, were evaluated to establish if the porins or β-lactamase in OMVs were involved in the degradation of β-lactam antibiotics. OMVs isolated from E. coli deficient in β-lactamase did not show any degradation ability against β-lactam antibiotics, while OMVs lacking OmpC or OmpF showed significantly lower levels of hydrolyzing activity than OMVs from parent E. coli. These data reveal an important role of OMVs in bacterial defense mechanisms demonstrating that the OmpC and OmpF proteins allow permeation of β-lactam antibiotics into the lumen of OMVs, and antibiotics that enter the OMVs can be degraded by β-lactamase.