bluuebunny/crossref_metadata_2025 huggingface dataset:
Cold Atmospheric Plasma-Treated PBS Eliminates Immunosuppressive Pancreatic Stellate Cells and Induces Immunogenic Cell Death of Pancreatic Cancer Cells
Authors: Van Loenhout Jinthe , Flieswasser Tal , Freire Boullosa Laurie , De Waele Jorrit , Van Audenaerde Jonas , Marcq Elly , Jacobs Julie , Lin Abraham , Lion Eva , Dewitte Heleen , Peeters Marc , Dewilde Sylvia , Lardon Filip , Bogaerts Annemie , Deben Christophe , Smits Evelien
Published: October 2019

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive cancers with a low response to treatment and a five-year survival rate below 5%. The ineffectiveness of treatment is partly because of an immunosuppressive tumor microenvironment, which comprises tumor-supportive pancreatic stellate cells (PSCs). Therefore, new therapeutic strategies are needed to tackle both the immunosuppressive PSC and pancreatic cancer cells (PCCs). Recently, physical cold atmospheric plasma consisting of reactive oxygen and nitrogen species has emerged as a novel treatment option for cancer. In this study, we investigated the cytotoxicity of plasma-treated phosphate-buffered saline (pPBS) using three PSC lines and four PCC lines and examined the immunogenicity of the induced cell death. We observed a decrease in the viability of PSC and PCC after pPBS treatment, with a higher efficacy in the latter. Two PCC lines expressed and released damage-associated molecular patterns characteristic of the induction of immunogenic cell death (ICD). In addition, pPBS-treated PCC were highly phagocytosed by dendritic cells (DCs), resulting in the maturation of DC. This indicates the high potential of pPBS to trigger ICD. In contrast, pPBS induced no ICD in PSC. In general, pPBS treatment of PCCs and PSCs created a more immunostimulatory secretion profile (higher TNF-α and IFN-γ, lower TGF-β) in coculture with DC. Altogether, these data show that plasma treatment via pPBS has the potential to induce ICD in PCCs and to reduce the immunosuppressive tumor microenvironment created by PSCs. Therefore, these data provide a strong experimental basis for further in vivo validation, which might potentially open the way for more successful combination strategies with immunotherapy for PDAC.
The Microenvironment of Pituitary Tumors—Biological and Therapeutic Implications
Authors: Ilie Mirela Diana , Vasiljevic Alexandre , Raverot Gérald , Bertolino Philippe
Published: October 2019

The tumor microenvironment (TME) includes resident and infiltrative non-tumor cells, as well as blood and lymph vessels, extracellular matrix molecules, and numerous soluble factors, such as cytokines and chemokines. While the TME is now considered to be a prognostic tool and a therapeutic target for many cancers, little is known about its composition in pituitary tumors. This review summarizes our current knowledge of the TME within pituitary tumors and the strong interest in TME as a therapeutic target. While we cover the importance of angiogenesis and immune infiltrating cells, we also address the role of the elusive folliculostellate cells, the emerging literature on pituitary tumor-associated fibroblasts, and the contribution of extracellular matrix components in these tumors. The cases of human pituitary tumors treated with TME-targeting therapies are reviewed and emerging concepts of vascular normalization and combined therapies are presented. Together, this snapshot overview of the current literature pinpoints not only the underestimated role of TME components in pituitary tumor biology, but also the major promise it may offer for both prognosis and targeted therapeutics.
Constitutive or Induced HIF-2 Addiction is Involved in Resistance to Anti-EGFR Treatment and Radiation Therapy in HNSCC
Authors: Coliat Pierre , Ramolu Ludivine , Jégu Jérémie , Gaiddon Christian , Jung Alain C. , Pencreach Erwan
Published: October 2019

Background: management of head and neck squamous cell carcinomas (HNSCC) include anti-Epidermal Growth Factor Receptor (EGFR) antibodies and radiotherapy, but resistance emerges in most patients. RAS mutations lead to primary resistance to EGFR blockade in metastatic colorectal cancer but are infrequent in HNSCC, suggesting that other mechanisms are implicated. Since hypoxia and Hypoxia Inducible Factor-1 (HIF-1) have been associated with treatment failure and tumor progression, we hypothesized that EGFR/mammalian Target of Rapamycin (mTOR)/HIF-1 axis inhibition could radiosensitize HNSCC. Methods: We treated the radiosensitive Cal27 used as control, and radioresistant SQ20B and UD-SCC1 cells, in vivo and in vitro, with rapamycin and cetuximab before irradiation and evaluated tumor progression and clonogenic survival. Results: Rapamycin and cetuximab inhibited the mTOR/HIF-1α axis, and sensitized the SQ20B cell line to EGFR-inhibition. However, concomitant delivery of radiation to SQ20B xenografts increased tumor relapse frequency, despite effective HIF-1 inhibition. Treatment failure was associated with the induction of HIF-2α expression by cetuximab and radiotherapy. Strikingly, SQ20B and UD-SCC1 cells clonogenic survival dropped <30% after HIF-2α silencing, suggesting a HIF-2-dependent mechanism of oncogenic addiction. Conclusions: altogether, our data suggest that resistance to EGFR inhibition combined with radiotherapy in HNSCC may depend on tumor HIF-2 expression and underline the urgent need to develop novel HIF-2 targeted treatments.
Effect of Carbon Fiber Addition on the Microstructure and Wear Resistance of Laser Cladding Composite Coatings
Authors: Li Jianfeng , Zhu Zhencai , Peng Yuxing , Shen Gang
Published: October 2019

In this study, the effect of carbon fibers (CFs) on the microstructure and wear resistance of Fe-based alloy coating produced by laser cladding was investigated by X-ray diffractometer (XRD), scanning electron microscopy (SEM), energy-dispersive spectrometer (EDS), and wear tester. The results indicated that with the addition of CFs, the microstructure of the composite coating mainly transformed from α-Fe cellular dendrites and γ-Fe/(Cr, Fe)7C3/CrB eutectics to bulk-like (Cr, Fe)7C3, nano-size B4C, and γ-(Fe, Ni)/(Cr, Fe)23C6 lamellar eutectics. Additionally, the microhardness and wear resistance of the composite coating compared with the original coating both increased by approximately two times. The original coating showed the dominant wear mechanisms of micro-cutting and serious brittle spalling, while the composite coating with CFs showed the main wear mechanism of slight scratching.
Evaluation of Uniformity of Bamboo Bundle Veneer and Bamboo Bundle Laminated Veneer Lumber (BLVL)
Authors: Zhou Haiying , Wei Xin , Smith Lee M. , Wang Ge , Chen Fuming
Published: October 2019

The lack of an effective and practical quality control method for industrialized bamboo bundle veneers is the key restriction in the application of bamboo bundle composite materials in the field of construction. In this work, the density uniformity and mechanical properties of bamboo bundle veneers were systematically evaluated by the combination of light transmittance and mechanical stiffness. It was found that the number of broomings, dippings, and high-temperature heat treatments had different effects on the bamboo bundle veneers. On this basis, the uniformity of the density and mechanical properties of the bamboo scrimber (BS) that underwent hybrid paving, and the bamboo bundle laminated veneer lumber (BLVL), were analyzed. The results showed that the performance stability of bamboo bundle composites could be greatly improved by bamboo bundle veneer laminated paving. A large-scale quality evaluation system for bamboo bundle veneers was established in this work, and it provides conditions for the manufacture of bamboo bundle composites with stable and controllable performance.
Comparison of Genetic Diversity in Naturally Regenerated Norway Spruce Stands and Seed Orchard Progeny Trials
Authors: Ruņģis Dainis , Luguza Solveiga , Bāders Endijs , Šķipars Vilnis , Jansons Āris
Published: October 2019

Forest ecosystems in Europe are expected to experience changes in temperature and water regimes associated with increased risks of extreme environmental events and disasters. Genetic diversity and relatedness has been linked to resilience of forest stands and landscapes. Genetic diversity indicators were compared between a Norway spruce population naturally regenerated after extensive windthrow and Norway spruce progeny populations derived from two seed orchards. In addition, genetic diversity in an undisturbed stand in a long established national park and a spruce genetic resource stand were analyzed. Populations were genotyped at 11 simple sequence repeat (SSR) loci. Average genetic diversity indicators were similar across populations. However, the total number of alleles, average number of alleles over all loci, effective number of alleles, average gene diversity, and average allelic richness were highest in the naturally regenerated population and lowest in one of the seed orchard progeny populations. The genetic diversity in progeny from seed orchards used for stand renewal is comparable to the genetic diversity in naturally regenerated stands. However, fluctuations in seed production between years can have a large impact on genetic diversity in seed orchard progeny. The use of improved Norway spruce germplasm deployed via clonal seed orchards for forest renewal can maintain similar levels of genetic diversity compared to naturally regenerated stands, while also increasing production and timber quality.
Phytophthora Species from Xinjiang Wild Apple Forests in China
Authors: Xu Xiaoxue , Huai Wenxia , Hamiti  , Zhang Xuechao , Zhao Wenxia
Published: October 2019

Phytophthora species are well-known destructive forest pathogens, especially in natural ecosystems. The wild apple (Malus sieversii (Ledeb.) Roem.) is the primary ancestor of M. domestica (Borkh.) and important germplasm resource for apple breeding and improvement. During the period from 2016 to 2018, a survey of Phytophthora diversity was performed at four wild apple forest plots (Xin Yuan (XY), Ba Lian (BL), Ku Erdening (KE), and Jin Qikesai (JQ)) on the northern slopes of Tianshan Mountain in Xinjiang, China. Phytophthora species were isolated from baiting leaves from stream, canopy drip, and soil samples and were identified based on morphological observations and the rDNA internal transcribed spacer (ITS) sequence analysis. This is the first comprehensive study from Xinjiang to examine the Phytophthora communities in wild apple forests The 621 resulting Phytophthora isolates were found to reside in 10 different Phytophthora species: eight known species (P. lacustris being the most frequent, followed by P. gonapodyides, P. plurivora, P. gregata, P. chlamydospora, P. inundata, P. virginiana, and P. cactorum) and two previously unrecognized species (P. sp. CYP74 and P. sp. forestsoil-like). The highest species richness of Phytophthora occurred at BL, followed by XY. P. lacustris was the dominant species at BL, XY, and JQ, while P. gonapodyides was the most common at KE. In the present paper, the possible reasons for their distribution, associated implications, and associated diseases are discussed.
The Forest Policies of ASEAN and Montréal Process: Comparing Highly and Weakly Formalized Regional Regimes
Authors: Jeon Sohui , Sarker Pradip Kumar , Giessen Lukas
Published: October 2019

Forests are governed by a combination of sub-national and national as well as global and regional regimes. Comparing the institutional variation of regional regimes, including their degrees of formalization, is gaining attention of studies on regionalism in International Relations. This study attempts to analyse the ways in which the selected cases of the forest-related Association of Southeast Asian Nations (ASEAN) and forest-focused Montréal Process (MP) regional regimes may have synergetic overlaps or disparity in their institutional design and forest policy development. For this, we combined IR’s ‘rational institutional design’ theory and a policy analysis approach. Using a qualitative data approach, we analyzed key structure-related historical regime documents (e.g., charters) issued since the inception of both regimes, and their latest forest policy initiatives for the periods 2016–2025 (Strategic Plan of Action for ASEAN Cooperation on Forestry) and 2009–2015 (Conceptual Framework for the Montréal Process Strategic Action Plan) with all relevant policy documents since the adoption of current policies. Based on that, we pose the empirical questions of how both regimes illustrate governance structure (i.e., institutional design), and on the other hand how to explain regime forest policies coherently and consistently in terms of their high versus low degree of formality. The results show that institutional design is highly explanatory based on treaty and non-treaty regime formation as well as forest-related/focused regime formation with the synergistic sustainable forest management (SFM) issue that embraces deforestation and forest degradation, biodiversity, timber certification, and greenhouse gas emission. Additionally, the results suggest that the policy goals adopted by both regimes are coherent and consistent based on the full set of policy elements. Concerning the remedy for fragmented global forest governance arrangements, both regimes would be an example of practicing SFM-focused policies with the incorporation of forest-related policy elements into a larger governance assemblage dealing with issues such as biodiversity conservation or climate change mitigation.
i6mA-DNCP: Computational Identification of DNA N6-Methyladenine Sites in the Rice Genome Using Optimized Dinucleotide-Based Features
Authors: Kong Liang , Zhang Lichao
Published: October 2019

DNA N6-methyladenine (6mA) plays an important role in regulating the gene expression of eukaryotes. Accurate identification of 6mA sites may assist in understanding genomic 6mA distributions and biological functions. Various experimental methods have been applied to detect 6mA sites in a genome-wide scope, but they are too time-consuming and expensive. Developing computational methods to rapidly identify 6mA sites is needed. In this paper, a new machine learning-based method, i6mA-DNCP, was proposed for identifying 6mA sites in the rice genome. Dinucleotide composition and dinucleotide-based DNA properties were first employed to represent DNA sequences. After a specially designed DNA property selection process, a bagging classifier was used to build the prediction model. The jackknife test on a benchmark dataset demonstrated that i6mA-DNCP could obtain 84.43% sensitivity, 88.86% specificity, 86.65% accuracy, a 0.734 Matthew’s correlation coefficient (MCC), and a 0.926 area under the receiver operating characteristic curve (AUC). Moreover, three independent datasets were established to assess the generalization ability of our method. Extensive experiments validated the effectiveness of i6mA-DNCP.
The Mechanisms of Mating in Pathogenic Fungi—A Plastic Trait
Authors: Usher Jane
Published: October 2019

The impact of fungi on human and plant health is an ever-increasing issue. Recent studies have estimated that human fungal infections result in an excess of one million deaths per year and plant fungal infections resulting in the loss of crop yields worth approximately 200 million per annum. Sexual reproduction in these economically important fungi has evolved in response to the environmental stresses encountered by the pathogens as a method to target DNA damage. Meiosis is integral to this process, through increasing diversity through recombination. Mating and meiosis have been extensively studied in the model yeast Saccharomyces cerevisiae, highlighting that these mechanisms have diverged even between apparently closely related species. To further examine this, this review will inspect these mechanisms in emerging important fungal pathogens, such as Candida, Aspergillus, and Cryptococcus. It shows that both sexual and asexual reproduction in these fungi demonstrate a high degree of plasticity.
Removal of Pollutants in Mine Wastewater by a Non-Cytotoxic Polymeric Bioflocculant from Alcaligenes faecalis HCB2
Authors: Maliehe Tsolanku Sidney , Basson Albertus Kotze , Dlamini Nkosinathi Goodman
Published: October 2019

Bioflocculation is a physicochemical technique often employed to efficiently remove colloidal water pollutants. Consequently, in this study, a bioflocculant was produced, characterised and applied to remove pollutants in mine wastewater. The maximum flocculation activity of 92% was recorded at 30 °C, pH 9.0 when maltose and urea were used as energy sources and 72 h of fermentation at the inoculum size of 1% (v/v). K+ proved to be a favourable cation. The bioflocculant yield of 4 g/L was obtained. Scanning electron microscopy illustrated a hexagonal-like structure of the bioflocculant. It is composed of carbohydrates and proteins in mass proportion of 88.6 and 9.5%, respectively. The Fourier transform infrared spectrum revealed the presence of hydroxyl, amide and amino functional groups. More than 73% of the bioflocculant was obtained after exposure to 600 °C using the thermogravimetric analyser. Human embryonic kidney 293 (HEK 293) cells exhibited 95% viability after being treated with 200 µg/µL of the bioflocculant. The flocculation mechanisms were proposed to be as a result of a double layer compression by K+, chemical reactions and bridging mechanism. The removal efficiencies of 59, 72, and 75% on biological oxygen demand, chemical oxygen demand and sulphur, were obtained respectively. Thus, the bioflocculant have potential use in wastewater treatment.
Parental Socialization, School Adjustment and Cyber-Aggression among Adolescents
Authors: Martínez-Ferrer Belén , León-Moreno Celeste , Musitu-Ferrer Daniel , Romero-Abrio Ana , Callejas-Jerónimo Juan , Musitu-Ochoa Gonzalo
Published: October 2019

The objective of the present study is to analyse the relationships between parental socialization styles—indulgent, authoritarian, authoritative and negligent, school adjustment (social integration, academic competence and family involvement) and cyber-aggression (direct and indirect) in adolescents. Participating in this study were 1304 Spanish students of both sexes (53.1% girls), aged between 12 and 18 years (M = 13.87, SD = 1.33). Multivariate analyses of variance were performed. The results showed significant relationships between parental socialization styles, school adjustment and cyber-aggression. It was observed that adolescents from indulgent and authoritative families showed greater academic competence and greater family involvement. Additionally, the children from authoritarian families displayed greater involvement in direct and indirect cyber-aggression behaviours. The results obtained and their implications are discussed in the final section.
A Risk-Averse Shelter Location and Evacuation Routing Assignment Problem in an Uncertain Environment
Authors: Liang Bian , Yang Dapeng , Qin Xinghong , Tinta Teresa
Published: October 2019

Disasters such as hurricanes, earthquakes and floods continue to have devastating socioeconomic impacts and endanger millions of lives. Shelters are safe zones that protect victims from possible damage, and evacuation routes are the paths from disaster zones toward shelter areas. To enable the timely evacuation of disaster zones, decisions regarding shelter location and routing assignment (i.e., traffic assignment) should be considered simultaneously. In this work, we propose a risk-averse stochastic programming model with a chance constraint that takes into account the uncertainty in the demand of disaster sites while minimizing the total evacuation time. The total evacuation time reflects the efficacy of emergency management from a system optimal (SO) perspective. A conditional value-at-risk (CVaR) is incorporated into the objective function to account for risk measures in the presence of uncertain post-disaster demand. We resolve the non-linear travel time function of traffic flow by employing a second-order cone programming (SOCP) approach and linearizing the non-linear chance constraints into a new mixed-integer linear programming (MILP) reformulation so that the problem can be directly solved by state-of-the-art optimization solvers. We illustrate the application of our model using two case studies. The first case study is used to demonstrate the difference between a risk-neutral model and our proposed model. An extensive computational study provides practical insight into the proposed modeling approach using another case study concerning the Black Saturday bushfire in Australia.
Periodontal Treatment Experience Associated with Oral Health-Related Quality of Life in Patients with Poor Glycemic Control in Type 2 Diabetes: A Case-Control Study
Authors: Hsu Yuan-Jung , Lin Kun-Der , Chen Jen-Hao , Lee Mei-Yueh , Lin Ying-Chu , Yen Feng-Chieh , Huang Hsiao-Ling
Published: October 2019

Severe periodontitis is a risk factor for poor glycemic control. The appropriate medical treatment and plaque control of periodontitis positively affects blood-sugar control in diabetes patients. We aimed to identify the factors associated with glycemic control and examine the periodontal treatment (PT) experience and oral health-related quality of life (OHQoL) for patients with poor glycemic control in type 2 diabetes mellitus (T2DM). This multicenter case–control study recruited 242 patients with poor glycemic control and 198 patients with good glycemic control. We collected patients’ information through face-to-face interviews using a structured questionnaire. The Oral Health Impact Profile-14 (OHIP-14) was used to measure OHQoL. Based on PT status, the patients were classified into three groups: a non-periodontal disease group, a PT group, and a non-PT (NPT) group. Regression models were used to analyze the data. No interdental cleaning (adjusted odds ratio (aOR) = 1.78) and positive attitudes toward periodontal health (aOR = 1.11) were significantly more likely to be associated with poor glycemic control in patients with T2DM. The PT group had a significantly lower OHIP-14 score than the NPT group (6.05 vs. 9.02, p < 0.001), indicating a better OHQoL among patients with poorly controlled T2DM. However, the OHQoL did not differ significantly in patients with well-controlled T2DM between the PT and NPT groups. This suggested that diabetic patients with poor glycemic control must improve periodontal care practices and receive proper PT, if necessary, to improve their OHQoL.
Health Inequality Among the Elderly in Rural China and Influencing Factors: Evidence from the Chinese Longitudinal Healthy Longevity Survey
Authors: Pan Changjian , Fan Qiuyan , Yang Jing , Deng Dasong
Published: October 2019

Based on data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS), this paper calculates the health distribution of the elderly using the Quality of Well-Being Scale (QWB) score, and then estimates health inequality among the elderly in rural China using the Wagstaff index (WI) and Erreygers index (EI). Following this, it compares health inequalities among the elderly in different age groups, and finally, uses the Shapley and recentered influence function-index-ordinary least squares (RIF-I-OLS) model to decompose the effect of four factors on health inequality among the elderly in rural China. The QWB score distribution shows that the health of the elderly in rural China improved with social economic development and medical reform from 2002 to 2014. However, at the same time, we were surprised to find that the health level of the 65–74 years old group has been declining steadily since 2008. This phenomenon implies that the incidence of chronic diseases is moving towards the younger elderly. The WI and EI show that there is indeed pro-rich health inequality among the rural elderly, the health inequality of the younger age groups is more serious than that of the older age groups, and the former incidence of health inequality is higher. Health inequality in the age group of 65–74 years old is higher than that in other groups, and the trend of change fluctuated downward from 2002 to 2014. Health inequality in the age group of 75–84 years old is lower than that in the group of 65–74 years old, but higher than that in the other age groups. The results of Shapley decomposition show that demographic characteristics, socioeconomic status (SES), health care access, and quality of later life contributed 0.0054, 0.0130, 0.0442, and 0.0218 to the health inequality index of the elderly, which accounted for 6.40%, 15.39%, 52.41%, and 25.80% of health inequality index. From the results of RIF-I-OLS decomposition, this paper has analyzed detailed factors’ marginal effects on health inequality from four dimensions, which indicates that the health inequality among the elderly in rural China was mainly caused by the disparity of income, medical expenses, and living arrangement.
Evaluation of the Effectiveness of Comprehensive Smoke-Free Legislation in Indoor Public Places in Shanghai, China
Authors: Wei Yanxia , Borland Ron , Zheng Pinpin , Fu Hua , Wang Fan , He Jingyi , Feng Yitian
Published: October 2019

This study evaluated compliance with the comprehensive smoke-free law in public indoor places introduced in Shanghai in March 2017. Observations and PM2.5 monitoring over 30 min intervals in 8 types of the venue were conducted three times: within a month before implementation and 3- and 12-months post implementation. Observations of evidence of smoking decreased from 66.2% before legislation to 52.8% three months after (p = 0.002) and 49.7% one year after (p < 0.001). The density of lit cigarettes also reduced significantly after implementation (p < 0.001). When adjusting for outdoor, indoor PM2.5 levels were significantly lower after the legislation, but only by a small amount (three months later: −0.27, p = 0.08; one year later: −0.12; p = 0.03). Evidence of compliance was weakest in farmer’s markets and bars, and smoking in male toilets did not change significantly. The reduction in smoking was affected by the management performance of their obligations. The comprehensive smoke-free law led to modest reductions in smoking and PM2.5 levels as a result, but from levels suggesting quite high levels of pre-compliance. However, compliance was limited in some areas, suggesting more effort is required on management to gain better compliance in some places like farmer’s markets, bars, and toilets.
Premature Mortality for Chronic Diseases in the EU Member States
Authors: Megyesiova Silvia , Lieskovska Vanda
Published: October 2019

Premature mortality, and especially premature mortality for chronic diseases, is a very important topic of public health, health care, or lifestyle of population. The main aim of countries is to reduce premature mortality, and therefore an analysis of the development and status of premature standardized death rates (SDR) is key for disclosure of successes or failures in this topic. A boxplot chart was used to detect extremes of SDR for both sexes. The gender ratio revealed the differences of mortality rates between men and women. Premature mortality declined steadily in the EU between 2000 and 2016. The men’s premature SDR decreased from 390 to 275.9 between 2000 and 2016, while the women’s rate declined from 180.1 to 138.2. On average, annual premature SDR dropped by 2.14% for men and 1.64% for women. Thus, the gender ratio (male/female) declined from 2.17 in 2000 to 2.0 in 2016, which is a positive change for gender gap closing. The highest proportion of premature mortality belonged to mortality for malignant neoplasms, where the rate was as high as 47% for women and 32% for men in 2016. Premature mortality for chronic disease is especially high in the “new” EU member states.
Mediating Effect of Social Support in the Relationship Between Childhood Abuse and Non-Suicidal Self-Injury Among Chinese Undergraduates: The Role of Only-Child Status
Authors: Xu Huiqiong , Song Xianbing , Wang Shanshan , Zhang Shichen , Xu Shaojun , Wan Yuhui
Published: October 2019

Previous research has found a relationship between child abuse and non-suicidal self-injury (NSSI). However, few studies have examined the role of social support underlying this association. Moreover, the influence of the only child status on the mediating effect of social support has not been studied yet. The aim of this study was to investigate the mediating role of social support on the association between specific forms of child abuse and NSSI as well as the role of the only child status on the mediated pathways, among undergraduates. A total of 4799 participants were selected from two medical colleges in the Anhui province using stratified cluster sampling. Pearson's correlation analysis was used in analyzing the relationship. Bootstrapping procedures were applied to examine the mediating effects. After adjusting for confounders, the results showed that the mediating effect of social support on the association between childhood abuse and NSSI was not significant in the total sample. However, among only children, the mediating effects of social support between overall childhood abuse, physical abuse, emotional abuse, and NSSI were 9.65%, 14.82%, and 8.12%, respectively. Moreover, the mediating effect of social support from family and relatives was relatively higher than that from other sources. Social support had a mediating effect on the relationship between childhood abuse and NSSI among only-children. The enhancing of social support may contribute to the prevention and control of NSSI for those who were only-children in undergraduates, especially those who have experienced childhood abuse.
Assessing the Spatial Distribution of Biodiversity in a Changing Temperature Pattern: The Case of Catalonia, Spain
Authors: Varga Diego , Roigé Mariona , Pintó Josep , Saez Marc
Published: October 2019

The impacts that climate change and land-use dynamics have on biodiversity are already visible in the distribution and behaviour of a large number of species. By using a Bayesian framework, including land-use, meteorological, topography and other variables as explanatory variables, such as distance to roads and urban centres, we modeled a number of species within each cell of a regular lattice for Catalonia, Spain, in the period of 2004 to 2010. We estimated a slight increase in daily maximum temperature and a more significant increase in minimum temperature (a 5-year increase of 0.159 °C in maximum temperature, and an increase of 0.332 °C in minimum temperature). The estimation shows that the total number of species was greater than expected in the cells where land use was not urban—38.4%, in forests and 55.2% in mixed forests. Finally, we observed that most invasive species are found in areas where the minimum temperature is expected to increase. Our study can help with making important recommendations as to where, when and how future threats could affect specie distribution and the kind of planning processes needed for when protected natural areas will be unable to continue to support all the species they were designed to protect.
Community-Based Healthcare Programs Sustainability Impact on the Sustainability of Host Organizations: A Structural Equation Modeling Analysis
Authors: Ceptureanu Sebastian Ion , Ceptureanu Eduard Gabriel
Published: October 2019

The sustainability of community-based programs represents a major focus of the literature on community-based interventions in the last few decades. However, without sustainable host organizations to effectively implement them, many are prone to failure. This paper analyzes the influence of the sustainability factors of healthcare community-based programs on the host organization’s sustainability. Based on a sample of 11 community-based healthcare programs and 401 respondents and using structural equation modeling, the study investigated if program specific, organization specific, and community specific factors are indeed measures of community-based programs’ sustainability, if social and economic dimensions are measures of host organization sustainability, and if the sustainability of the community-based program influences thee host organization’s sustainability. The results confirmed all three research hypothesis. The main contribution of the paper is to demonstrate a direct relationship between the sustainability of community-based programs and the overall sustainability of the organizations implementing them.
Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action
Authors: Rosa Brenda , Marchetti Marialaura , Paredi Gianluca , Amenitsch Heinz , Franko Nina , Benoni Roberto , Giabbai Barbara , De Marino Maria Giovanna , Mozzarelli Andrea , Ronda Luca , Storici Paola , Campanini Barbara , Bettati Stefano
Published: October 2019

The formation of multienzymatic complexes allows for the fine tuning of many aspects of enzymatic functions, such as efficiency, localization, stability, and moonlighting. Here, we investigated, in solution, the structure of bacterial cysteine synthase (CS) complex. CS is formed by serine acetyltransferase (CysE) and O-acetylserine sulfhydrylase isozyme A (CysK), the enzymes that catalyze the last two steps of cysteine biosynthesis in bacteria. CysK and CysE have been proposed as potential targets for antibiotics, since cysteine and related metabolites are intimately linked to protection of bacterial cells against redox damage and to antibiotic resistance. We applied a combined approach of small-angle X-ray scattering (SAXS) spectroscopy and protein painting to obtain a model for the solution structure of CS. Protein painting allowed the identification of protein–protein interaction hotspots that were then used as constrains to model the CS quaternary assembly inside the SAXS envelope. We demonstrate that the active site entrance of CysK is involved in complex formation, as suggested by site-directed mutagenesis and functional studies. Furthermore, complex formation involves a conformational change in one CysK subunit that is likely transmitted through the dimer interface to the other subunit, with a regulatory effect. Finally, SAXS data indicate that only one active site of CysK is involved in direct interaction with CysE and unambiguously unveil the quaternary arrangement of CS.
Bangla DeConverter for Extraction of BanglaText from Universal Networking Language
Authors: Ali Md. Nawab Yousuf , Rahman Md. Lizur , Sorwar Golam
Published: October 2019

The people in Bangladesh and two states (i.e., Tripura and West Bengal) in India, which is about 230 million of the world population, use Bangla as their first dialect. However, very few numbers of resources and tools are available for this language. This paper presents a Bangla DeConverter to extract Bangla texts from Universal Networking Language (UNL). It explains and illustrates the different phases of the proposed Bangla DeConverter. The syntactic linearization, the implementation of the results of the proposed Bangla DeConverter, and the extraction of a Bangla sentence from UNL expressions are presented in this paper. The Bangla DeConverter has been tested on UNL expressions of 300 Bangla sentences using a Russian and English Language Server. The proposed system generates 90% syntactically and semantically correct Bangla sentences with a UNL Bilingual Evaluation Understudy (BLEU) score of 0.76.
The Effect of Withdrawal Rate on Crystal Structure Perfection, Microstructure and Creep Resistance of Single Crystal Castings Made of CMSX-4 Nickel-Based Superalloy
Authors: Gancarczyk Kamil , Zubko Maciej , Hanc-Kuczkowska Aneta , Kościelniak Barbara , Albrecht Robert , Szeliga Dariusz , Motyka Maciej , Ziaja Waldemar , Sieniawski Jan
Published: October 2019

This study focuses on the evaluation of the crystal structure perfection in the single crystal made of CMSX-4 nickel superalloy and its effect on creep resistance. Single crystal castings were manufactured by directional solidification process at the withdrawal rate of 1, 3, 5 and 7 mm/min. Light (LM) and electron (SEM, TEM) microscopy, X-ray diffraction and Mossbauer spectroscopy were used for evaluation of the microstructure and crystal structure perfection. Castings were also subjected to creep tests. The best creep resistance was obtained for the casting manufactured at the withdrawal rate of 3 mm/min, characterized by the highest crystal structure perfection compared to the other castings examined.
A Comparison of the Compositional, Microstructural, and Mechanical Characteristics of Ni-Free and Conventional Stainless Steel Orthodontic Wires
Authors: Brüngger Daniela , Koutsoukis Theodoros , S. Al Jabbari Youssef , Hersberger-Zurfluh Monika , Zinelis Spiros , Eliades Theodore
Published: October 2019

Ni-free orthodontic wires were introduced to mitigate concerns associated with the use of Ni-containing alloys in orthodontics. However, limited information is available on their properties and therefore, the aim of this study was to characterize the elemental composition, the microstructure, and the mechanical properties of Ni-free orthodontic wires and compare them with their stainless steel (SS) counterparts. Four Ni-free and four conventional SS wires were included in this study. All the wires were initially imaged with a Scanning Electron Microscopy (SEM) and their elemental compositions were determined by X-ray Energy Dispersive Spectroscopy (EDX). Then, their microstructure was assessed by X-ray Diffraction (XRD) and the indentation modulus, elastic index, Martens Hardness and Vickers Hardness by Instrumented Indentation Testing (IIT). All the wires demonstrated surface cracks and pores oriented parallel to their long axis. The elemental composition of Ni-free alloys showed an increased Mn and Cr content while both SS and Ni-free wires shared the same dominant austenite structure. In conclusion, despite the differences in elemental composition, Ni-free wires demonstrated a similar microstructure and comparable mechanical properties with their conventional SS counterparts and thus may be considered as a promising alternative for patients with Ni supersensitivity.
Possibilities of Manufacturing Products from Cermet Compositions Using Nanoscale Powders by Additive Manufacturing Methods
Authors: Grigoriev Sergei , Tarasova Tatiana , Gusarov Andrey , Khmyrov Roman , Egorov Sergei
Published: October 2019

Complicated wear-resistant parts made by selective laser melting (SLM) of powder material based on compositions of metal and ceramics can be widely used in mining, oil engineering, and other precision engineering industries. Ceramic–metal compositions were made using nanoscale powders by powder metallurgy methods. Optimal regimes were found for the SLM method. Chemical and phase composition, fracture toughness, and wear resistance of the obtained materials were determined. The wear rate of samples from 94 wt% tungsten carbide (WC) and 6 wt% cobalt (Co) was 1.3 times lower than that of a sample from BK6 obtained by the conventional methods. The hardness of obtained samples 2500 HV was 1.6 times higher than that of a sample from BK6 obtained by the traditional method (1550 HV).
Femtosecond Laser Fabrication of Stable Hydrophilic and Anti-Corrosive Steel Surfaces
Authors: Lanara Christina , Mimidis Alexandros , Stratakis Emmanuel
Published: October 2019

We report on a novel single-step method to develop steel surfaces with permanent highly hydrophilic and anti-corrosive properties, without employing any chemical coating. It is based on the femtosecond (fs) laser processing in a saturated background gas atmosphere. It is particularly shown that the fs laser microstructuring of steel in the presence of ammonia gas gives rise to pseudoperiodic arrays of microcones exhibiting highly hydrophilic properties, which are stable over time. This is in contrast to the conventional fs laser processing of steel in air, which always provides surfaces with progressively increasing hydrophobicity following irradiation. More importantly, the surfaces subjected to fs laser treatment in ammonia exhibit remarkable anti-corrosion properties, contrary to those processed in air, as well as untreated ones. The combination of two functionalities, namely hydrophilicity and corrosion resistance, together with the facile processing performed directly onto the steel surface, without the need to deposit any coating, opens the way for the laser-based production of high-performance steel components for a variety of applications, including mechanical parts, fluidic components and consumer products.
Evaluation of the Microstructure and Mechanical Properties of a New Modified Cast and Laser-Melted AA7075 Alloy
Authors: Khalil Asmaa M. , Loginova Irina S. , Pozdniakov Andrey V. , Mosleh Ahmed O. , Solonin Alexey N.
Published: October 2019

The mechanical properties and microstructure of as-cast and homogenized AA7075 were investigated. This alloy was modified by adding transition elements 0.3%Sc + 0.5%Zr, 1%Ti + 0.2%B, and 1%Fe + 1%Ni for use in additive manufacturing applications. After adding Ti + B and Sc + Zr, the structure became uniform and finer with the formation of the Al3(Sc, Zr) and TiB2 phases. Coarse structures were obtained with the formation of an extremely unfavorable morphology, close to a needle-like structure when Fe + Ni was added. The mechanical properties of the modified alloys were increased compared to those of the standard alloy, where the best ultimate tensile strength (UTS) and yield strength (YS) were obtained in the AA7075-TiB alloy compared to the standard alloy in as-cast and homogenized conditions, and the highest hardness value was provided by Fe + Ni additives. The effect of the laser melting process on the microstructure and mechanical properties was investigated. Single laser melts were performed on these alloys using 330 V and a scanning speed of 8 mm/s. During the laser melting, the liquation of the alloying elements occurred due to non-equilibrium solidification. A change in the microstructures was observed within the melt zone and heat-affected zone (HAZ). The hardness of the laser-melted zone (LMZ) after adding the modification elements was increased in comparison with that of the standard alloy. Corrosion testing was performed using a solution of 100 mL distilled water, 3.1 g NaCl, and 1 mL HCl over 5, 10, and 30 min and 1 and 2 h. The corrosion resistance of the alloy modified with FeNi was low because of the non-uniform elemental distribution along the LMZ, but in the case of modification with ScZr and TiB, the corrosion resistance was better compared to that of the standard alloy.
Fabrication of Superhydrophobic Magnetic Sawdust as Effective and Recyclable Oil Sorbents
Authors: Fan Shumin , Pei Shuai , Shen Tianyu , Xu Guangri , Li Yuanchao , Fan Wenxiu
Published: October 2019

In this paper, a novel superhydrophobic magnetic sawdust (SMSD) was fabricated as an oil sorbent. The SMSD was functionalized with Fe3O4 nanoparticles using melamine formaldehyde resin (MFR) as a coupling agent and subsequently hydrophobically-treated with hexadecyltrimethoxysilane (HDTMS). The SMSD showed excellent superhydrophobicity with the water contact angle of 155.3 ± 0.9°. Meanwhile it had remarkable environmental durability, long-term stability, and mechanical durable properties. Taking advantage of its magnetic characteristics, the SMSD could be easily controlled to absorb oil to separate oil–water mixtures with high oil absorption capacity and good reusability. Moreover, the emulsion was successfully separated by SMSD, including water-in-oil and oil-in-water emulsions. This study developed an effective oil absorbent, which was low cost and environmentally-friendly.
Physical Properties of Mineral and Recycled Aggregates Used to Mineral-Asphalt Mixtures
Authors: Andrzejuk Wojciech , Barnat-Hunek Danuta , Góra Jacek
Published: October 2019

This article presents test results and examines the possibilities of using aggregate from ceramic waste for mineral-asphalt mixtures. In addition, the mineral composition, physical and mechanical properties of aggregates from natural raw materials such as dolomite, granodiorite and waste ceramic aggregate (introduced as a partial substitute for the main aggregate) were analyzed. The shape of grains was examined by determining the shape and flatness index of aggregates, resistance to grinding and frost resistance. The tested properties have a direct impact on the durability of road surfaces. To this end, the adhesion of asphalt to the surface of the aggregates used was additionally determined. Determination of surface roughness and two-dimensional (2D) topography of tested aggregates was carried out. The aggregates microstructure examination, coupled with the energy-dispersive X-ray spectroscopy (EDS) analysis, was conducted to determine the morphology and texture of the aggregates as well as to identify the basic chemical components.
Novel Poly(Methyl Methacrylate) Containing Nanodiamond to Improve the Mechanical Properties and Fungal Resistance
Authors: Mangal Utkarsh , Kim Ji-Yeong , Seo Ji-Young , Kwon Jae-Sung , Choi Sung-Hwan
Published: October 2019

Herein we evaluate the effect of nanodiamond (ND) incorporation on the mechanical properties of poly(methyl methacrylate) (PMMA) nanocomposite. Three quantities of ND (0.1, 0.3, and 0.5 wt.%) were tested against the control and zirconium oxide nanoparticles (ZrO). Flexural strength and elastic modulus were measured using a three-point bending test, surface hardness was evaluated using the Vickers hardness test, and surface roughness was evaluated using atomic force microscopy (AFM), while fungal adhesion and viability were studied using Candida albicans. Samples were also analyzed for biofilm thickness and biomass in a saliva-derived biofilm model. All groups of ND-PMMA nanocomposites had significantly greater mean flexural strengths and statistically improved elastic modulus, compared to the control and ZrO groups (P < 0.001). The Vickers hardness values significantly increased compared to the control group (P < 0.001) with 0.3% and 0.5% ND. ND addition also gave significant reduction in fungal adhesion and viability (P < 0.001) compared to the control group. Finally, salivary biofilm formation was markedly reduced compared to the ZrO group. Hence, the incorporation of 0.1–0.5 wt.% ND with auto- polymerized PMMA resin significantly improved the flexural strength, elastic modulus, and surface hardness, and provided considerable fungal resistance.
Parameter Optimization in High-Throughput Testing for Structural Materials
Authors: Bader Alexander , Toenjes Anastasiya , Wielki Nicole , Mändle Andreas , Onken Ann-Kathrin , Hehl Axel von , Meyer Daniel , Brannath Werner , Tracht Kirsten
Published: October 2019

High-throughput screenings are established evaluation methods in the development of functional materials and pharmaceutical active ingredients. The transfer of this approach to the development of structural materials requires extensive adaptations. In addition to the investigation of new test procedures for the determination of material properties and the treatment of metallic materials, the design of experiments is a research focus. Based on given descriptor target values, the statistical design of experiments determines investigations and treatments for the investigation of these materials. In this context, process parameters also have to be determined, as these have a major influence on the later material properties, especially during the treatment of samples. In this article, a method is presented which determines the process parameters iteratively. The validation of the calculated process parameters takes place based on differential scanning calorimetry used as the furnace for the heat treatment of small batches and particle-oriented peening as the characterization method.