bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and Tobago
Authors: Khan Anisa Sarah , Pierneef Rian Ewald , Gonzalez-Escalona Narjol , Maguire Meghan , Li Cong , Tyson Gregory H. , Ayers Sherry , Georges Karla , Abebe Woubit , Adesiyun Abiodun Adewale
Published: March 2022

This cross-sectional study determined the serovars, antimicrobial resistance genes, and virulence factors of Salmonella isolated from hatcheries, broiler farms, processing plants, and retail outlets in Trinidad and Tobago. Salmonella in silico serotyping detected 23 different serovars where Kentucky 20.5% (30/146), Javiana 19.2% (28/146), Infantis 13.7% (20/146), and Albany 8.9% (13/146) were the predominant serovars. There was a 76.0% (111/146) agreement between serotyping results using traditional conventional methods and whole-genome sequencing (WGS) in in silico analysis. In silico identification of antimicrobial resistance genes conferring resistance to aminoglycosides, cephalosporins, peptides, sulfonamides, and antiseptics were detected. Multidrug resistance (MDR) was detected in 6.8% (10/146) of the isolates of which 100% originated from broiler farms. Overall, virulence factors associated with secretion systems and fimbrial adherence determinants accounted for 69.3% (3091/4463), and 29.2% (1302/4463) counts, respectively. Ten of 20 isolates of serovar Infantis (50.0%) showed MDR and contained the blaCTX-M-65 gene. This is the first molecular characterization of Salmonella isolates detected along the entire broiler production continuum in the Caribbean region using WGS. The availability of these genomes will help future source tracking during epidemiological investigations associated with Salmonella foodborne outbreaks in the region and worldwide.
Gut Microbial Characterization of Melon-Headed Whales (Peponocephala electra) Stranded in China
Authors: Bai Shijie , Zhang Peijun , Zhang Xianfeng , Yang Zixin , Li Songhai
Published: March 2022

Although gut microbes are regarded as a significant component of many mammals and play a very important role, there is a paucity of knowledge around marine mammal gut microbes, which may be due to sampling difficulties. Moreover, to date, there are very few, if any, reports on the gut microbes of melon-headed whales. In this study, we opportunistically collected fecal samples from eight stranded melon-headed whales (Peponocephala electra) in China. Using high-throughput sequencing technology of partial 16S rRNA gene sequences, we demonstrate that the main taxa of melon-headed whale gut microbes are Firmicutes, Fusobacteriota, Bacteroidota, and Proteobacteria (Gamma) at the phylum taxonomic level, and Cetobacterium, Bacteroides, Clostridium sensu stricto, and Enterococcus at the genus taxonomic level. Meanwhile, molecular ecological network analysis (MENA) shows that two modules (a set of nodes that have strong interactions) constitute the gut microbial community network of melon-headed whales. Module 1 is mainly composed of Bacteroides, while Module 2 comprises Cetobacterium and Enterococcus, and the network keystone genera are Corynebacterium, Alcaligenes, Acinetobacter, and Flavobacterium. Furthermore, by predicting the functions of the gut microbial community through PICRUSt2, we found that although there are differences in the composition of the gut microbial community in different individuals, the predicted functional profiles are similar. Our study gives a preliminary inside look into the composition of the gut microbiota of stranded melon-headed whales.
The Features of Distribution of Chemical Elements, including Heavy Metals and Cs-137, in Surface Sediments of the Barents, Kara, Laptev and East Siberian Seas
Authors: Budko Dmitry F. , Demina Liudmila L. , Travkina Anna V. , Starodymova Dina P. , Alekseeva Tatiyana N.
Published: March 2022

Over the recent few decades, due to climate warming and the continuing exploration of Arctic seas’ mineral resources, the scientific interest in contamination problems has deepened significantly. In this study, for the first time, we characterize the distribution features of 47 elements (major and trace elements, including heavy metals, metalloid As, and Cs-137 technogenic radionuclide) in surface bottom sediments from some areas of the Barents, Kara, Laptev, and East-Siberian Seas. The lithogenic material was the main factor that controlled variability in many elements (Be, Al, Ti, Cr, Ga, Rb, Sr, Y, Zr, Nb, Ba, REE, Pb, Th, U, W, and Cs). Among the hydrogenic processes, the formation of Fe and Mn oxyhydroxides has the greatest impact on the Mn, Fe, Co, Ni, Cu, Ge, and Mo, and insignificantly V and Sb, variability in sediments. These, along with minor to moderate values of enrichment factor (EF) for most elements, allowed us to conclude that the observed element distribution is related to predominantly natural processes of thermal abrasion, river-run, and atmospheric input. The exception is As, which exhibited the elevated EF (up to 20) in the western and central Kara Sea, as well as in the Vilkitsky Strait. Since no significant relationship between As and Fe andMn oxyhydroxides distribution was found, we may assume primarily an anthropogenic source of As, related to the peat and/or coal combustion. According to the criteria of Ecological Risks assessment, all the examined areas have a low degree of risk. Data on the specific activity of Cs-137 correspond to the background average values characteristic for these regions. The highest levels of Cs-137 concentration (Bq/kg) were detected in the sediments of the Ob and Yenisei Rivers’ estuaries.
Multi-Residue Analysis of Chemical Additives in Edible Vegetable Oils Using QuEChERS Extraction Method Followed by Supercritical Fluid Chromatography
Authors: Gan Yaping , Zhu Yan
Published: March 2022

Since the quality and safety of food highly depend on its preservation and protection, the use of food packaging materials increases the risk of chemical contamination of the packaged food by migration. Herein, we focused on antioxidants, photoinitiators, UV absorbers and plasticizers which are extensive additives used in food packaging materials. In the present study, a rapid, simple, green and reliable method was developed and validated for the determination of twelve chemical additives in edible vegetable oils using SFC together with a modified QuEChERS procedure. Under the optimum conditions, twelve additives were separated within 10 min, and the consumption of the organic solvent was significantly reduced, which improved the environmentally friendliness. The performance of the developed method was evaluated. Good linearity (r > 0.999) was obtained in the range of 0.20–20.0 µg/mL and 0.50–20.0 µg/mL, respectively. The limits of detection and limits of quantification of the twelve additives in vegetable oils were 0.05–0.15 µg/mL and 0.15–0.50 µg/mL, respectively. Recoveries of all the chemical additives for the spiked samples were between 60.9% and 106.4%, with relative standard deviations (RSD) lower than 9.9%. The results demonstrated that the proposed method was efficient, reliable and robust for the routine analysis of additives in edible vegetable oils and can be an alternative to the multi-residue analysis of chemical additives for other packaged foods.
New Pharmacological Strategies against Pancreatic Adenocarcinoma: The Multifunctional Thiosemicarbazone FA4
Authors: Anobile Dario P. , Niso Mauro , Puerta Adrian , Fraga Rodrigues Stephanie M. , Abatematteo Francesca S. , Avan Amir , Abate Carmen , Riganti Chiara , Giovannetti Elisa
Published: March 2022

A new sigma-2 (σ2) receptor ligand (FA4) was efficiently synthesized and evaluated for cytotoxic, proapoptotic, and antimigratory activity on pancreatic ductal adenocarcinoma (PDAC) primary cell cultures, which restrained the aggressive and chemoresistant behavior of PDAC. This compound showed relevant antiproliferative activity with half maximal inhibitory concentration (IC50) values ranging from 0.701 to 0.825 μM. The cytotoxic activity was associated with induction of apoptosis, resulting in apoptotic indexes higher than those observed after exposure to a clinically relevant concentration of the gemcitabine, the first-line drug used against PDAC. Interestingly, FA4 was also able to significantly inhibit the migration rate of both PDAC-1 and PDAC-2 cells in the scratch wound-healing assay. In conclusion, our results support further studies to improve the library of thiosemicarbazones targeting the σ-2 receptor for a deeper understanding of the relationship between the biological activity of these compounds and the development of more efficient anticancer compounds against PDAC.
Synthesis of Mono-Amino Substituted γ-CD: Host–Guest Complexation and In Vitro Cytotoxicity Investigation
Authors: Odeh Fadwa , Adaileh Fedaa , Alshaer Walhan , Nsairat Hamdi , Alqudah Dana A. , Jaber Areej M. , Al Bawab Abeer
Published: March 2022

Cyclodextrins (CDs) are cyclic oligosaccharides which can trap hydrophobic molecules and improve their chemical, physical, and biological properties. γ-CD showed the highest aqueous solubility with the largest cavity diameter among other CD types. The current study describes a direct and easy method for nucleophilic mono-aminos to be substituted with γ-CD and tested for their ability to host the guest curcumin (CUR) as a hydrophobic drug model. The mass spectrometry and NMR analyses showed the successful synthesis of three amino-modified γ-CDs: mono-6-amino-6-deoxy-cyclodextrine (γ-CD-NH2), mono-6-deoxy-6-ethanolamine-γ-cyclodextrine (γ-CD-NHCH2CH2OH), and mono-6-deoxy-6-aminoethylamino)-γ-cyclodextrin (γ-CD-NHCH2CH2NH2). These three amino-modified γ-CDs were proven to be able to host CUR as native γ-CDs with formation constants equal to 6.70 ± 1.02, 5.85 ± 0.80, and 8.98 ± 0.90 mM−1, respectively. Moreover, these amino-modified γ-CDs showed no significant toxicity against human dermal fibroblast cells. In conclusion, the current work describes a mono-substitution of amino-modified γ-CDs that can still host guests and showed low toxicity in human dermal fibroblasts cells. Therefore, the amino-modified γ-CDs can be used as a carrier host and be conjugated with a wide range of molecules for different biomedical applications, especially for active loading methods.
CO2 to CO Electroreduction, Electrocatalytic H2 Evolution, and Catalytic Degradation of Organic Dyes Using a Co(II) meso-Tetraarylporphyrin
Authors: Guergueb Mouhieddinne , Loiseau Frédérique , Molton Florian , Nasri Habib , Klein Axel
Published: March 2022

The meso-tetrakis(4-(trifluoromethyl)phenyl)porphyrinato cobalt(II) complex [Co(TMFPP)] was synthesised in 93% yield. The compound was studied by 1H NMR, UV-visible absorption, and photoluminescence spectroscopy. The optical band gap Eg was calculated to 2.15 eV using the Tauc plot method and a semiconducting character is suggested. Cyclic voltammetry showed two fully reversible reduction waves at E1/2 = −0.91 V and E1/2 = −2.05 V vs. SCE and reversible oxidations at 0.30 V and 0.98 V representing both metal-centred (Co(0)/Co(I)/Co(II)/Co(III)) and porphyrin-centred (Por2−/Por−) processes. [Co(TMFPP)] is a very active catalyst for the electrochemical formation of H2 from DMF/acetic acid, with a Faradaic Efficiency (FE) of 85%, and also catalysed the reduction of CO2 to CO with a FE of 90%. Moreover, the two triarylmethane dyes crystal violet and malachite green were decomposed using H2O2 and [Co(TMFPP)] as catalyst with an efficiency of more than 85% in one batch.
Improved Interfacial Contact for Pyramidal Texturing of Silicon Heterojunction Solar Cells
Authors: Dai Ruijie , Huang Tengzuo , Zhou Weijie , Yang Jinpeng , Zhang Hua , Yu Fayin , Chen Anran , Wang Feng , Zhang Jin , Sun Tao , Zhang Longzhou
Published: March 2022

Reducing the surface reflectivity of silicon substrates is essential for preparing high-performance Si-based solar cells. We synthesized pyramid-nanowire-structured Si (Si-PNWs) anti-reflection substrates, which have excellent light-trapping ability (<4% reflectance). Furthermore, diethyl phthalate (DEP), a water-insoluble phthalic acid ester, was applied to optimize the Si-PNWs/PEDOT:PSS interface; the photoelectric conversion efficiency of heterojunction solar cells was shown to increase from 9.82% to 13.48%. We performed a detailed examination of the shape and optical characteristics of Si-PNWs, as well as associated photoelectric performance tests, to investigate the origin of performance improvements in Si-PNWs/PEDOT:PSS heterojunction solar cells (HSCs).
Ionic Liquid-Based Polyoxometalate Incorporated at ZIF-8: A Sustainable Catalyst to Combine Desulfurization and Denitrogenation Processes
Authors: Silva Dinis F. , Viana Alexandre M. , Santos-Vieira Isabel , Balula Salete S. , Cunha-Silva Luís
Published: March 2022

An effective and sustainable process capable of simultaneously execute desulfurization and denitrogenation of fuels is in fact an actual necessity in the refinery industry. The key to achieve this goal is the parallel oxidation of sulfur and nitrogen compounds present in fuels, which is only achieved by an active and recovered catalyst. A novel heterogeneous catalyst was successfully prepared by the encapsulation of an imidazolium-based polyoxometalate (POM) into a ZIF-8 framework ([BMIM]PMo12@ZIF-8). This composite material revealed exceptional catalytic efficiency to concurrently proceed with the oxidative desulfurization and denitrogenation of a multicomponent model fuel containing various sulfur and nitrogen compounds. A complete removal of all these compounds was achieved after only one hour and the catalyst system was able to be reused for ten consecutive cycles without loss of efficiency. In fact, an ionic liquid POM was incorporated in the ZIF-8 for the first time, and this composite compound was originally applied as a catalyst for simultaneous oxidative desulfurization and denitrogenation processes.
Evaluating the Conservation State of Naturally Aged Paper with Raman and Luminescence Spectral Mapping: Toward a Non-Destructive Diagnostic Protocol
Authors: Botti Sabina , Bonfigli Francesca , Nigro Valentina , Rufoloni Alessandro , Vannozzi Angelo
Published: March 2022

Micro-Raman and luminescence spectroscopy were combined with morphological analysis to study the conservation state of differently degraded paper samples, dated from 1873 to 2021. The aim of the work reported in this paper was to obtain ageing markers based on variations of Raman and fluorescence spectral features. Raman and luminescence spectra were acquired by scanning non-printed areas of books, and Raman and fluorescence maps were built by contrasting spectral parameters point by point, obtaining a micron-scale space resolved imaging of the degradation pattern. Complementary information on paper morphology and surface compactness were obtained by high-resolution scanning electron and atomic force microscopy. The proposed non-destructive procedure is particularly interesting for precious and ancient samples to analyze their degradation processes and to evaluate the performance and effectiveness of restoration treatments.
Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice
Authors: Khan Asif Iqbal , Rehman Ata Ur , Farooqui Nabeel Ahmed , Siddiqui Nimra Zafar , Ayub Qamar , Ramzan Muhammad Noman , Wang Liang , Xin Yi
Published: March 2022

The gut microbiota is important in regulating host metabolism, maintaining physiology, and protecting immune homeostasis. Gut microbiota dysbiosis affects the development of the gut microenvironment, as well as the onset of various external systemic diseases and metabolic syndromes. Cyclophosphamide (CTX) is a commonly used chemotherapeutic drug that suppresses the host immune system, intestinal mucosa inflammation, and dysbiosis of the intestinal flora. Immunomodulators are necessary to enhance the immune system and prevent homeostasis disbalance and cytotoxicity caused by CTX. In this study, shrimp peptide hydrolysate (SPH) was evaluated for immunomodulation, intestinal integration, and microbiota in CTX-induced immunosuppressed mice. It was observed that SPH would significantly restore goblet cells and intestinal mucosa integrity, modulate the immune system, and increase relative expression of mRNA and tight-junction associated proteins (Occludin, Zo-1, Claudin-1, and Mucin-2). It also improved gut flora and restored the intestinal microbiota ecological balance by removing harmful microbes of various taxonomic groups. This would also increase the immune organs index, serum levels of cytokines (IFN-ϒ, IL1β, TNF-α, IL-6), and immunoglobin levels (IgA, IgM). The Firmicutes/Bacteroidetes proportion was decreased in CTX-induced mice. Finally, SPH would be recommended as a functional food source with a modulatory effect not only on intestinal microbiota, but also as a potential health-promoting immune function regulator.
Wound Healing, Antimicrobial and Antioxidant Properties of Clinacanthus nutans (Burm.f.) Lindau and Strobilanthes crispus (L.) Blume Extracts
Authors: Ban Weng Kit , Fong Isabel Lim , Khong Heng Yen , Phung Joyce Hui Yie
Published: March 2022

Clinacanthus nutans is known to be an anticancer and antiviral agent, and Strobilanthes crispus has proven to be an antidiuretic and antidiabetic agent. However, there is a high possibility that these plants possess multiple beneficial properties, such as antimicrobial and wound healing properties. This study aims to assess the wound healing, antioxidant, and antimicrobial properties of Clinacanthus nutans and Strobilanthes crispus. The Clinacanthus nutans and Strobilanthes crispus leaves were dried, ground, and extracted with ethanol, acetone, and chloroform through cold maceration. In a modified scratch assay with co-incubation of skin fibroblast and Methicillin-resistant Staphylococcus aureus, Clinacanthus nutans and Strobilanthes crispus extracts were assessed for their wound healing potential, and the antimicrobial activities of Clinacanthus nutans and Strobilanthes crispus extracts were performed on a panel of Gram-positive and Gram-negative bacteria on Mueller–Hinton agar based on a disc diffusion assay. To assess for antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and total flavonoid assays were conducted. In the modified scratch assay, Clinacanthus nutans extracts aided in the wound healing activity while in the presence of MRSA, and Strobilanthes crispus extracts were superior in antimicrobial and wound healing activities. In addition, Strobilanthes crispus extracts were superior to Clinacanthus nutans extracts against Pseudomonas aeruginosa on Mueller–Hinton agar. Acetone-extracted Clinacanthus nutans contained the highest level of antioxidant in comparison with other Clinacanthus nutans extracts.
Poly(vinylbenzyl Pyridinium Salts) as Novel Sorbents for Hazardous Metals Ions Removal
Authors: Wieszczycka Karolina , Filipowiak Kinga , Lewandowska Aneta , Marcinkowska Agnieszka , Nowicki Marek
Published: March 2022

Novel efficient complexing resins—poly(vinylbenzyl pyridinium salts) fabricated through poly(vinylbenzyl halogene-co-divinylbenzene) quaternization of N-decyloxy-1-(pyridin-3-yl)ethaneimine and N-decyloxy-1-(pyridin-4-yl)ethaneimine—were tested as adsorbents of Pb(II), Cd(II), Cu(II), Zn(II), and Ni(II) from aqueous solutions. The structure of these materials was established by 13C CP-MAS NMR, X-ray photoelectron spectroscopy, elemental analysis, and Fourier transform infrared spectroscopy, as well as thermogravimetric and differential thermal analyses. The textural properties were determined using scanning electron microscopy and low-temperature N2 sorption. Based on the conducted sorption studies, it was shown that the uptake behavior of the metal ions towards novel resins depended on the type of functionalities, contact time, pH, metal concentrations, and the resin dosage. The Langmuir model was investigated to be the best one for fitting isothermal adsorption equilibrium data, and the corresponding adsorption capacities were predicted to be 296.4, 201.8, 83.8, 38.1, and 39.3 mg/g for Pb(II), Zn(II), Cd(II), Cu(II), and Ni(II), respectively. These results confirmed that owing to the presence of the functional pyridinium groups, the resins demonstrated proficient metal ion removal capacities. Furthermore, VBBr-D4EI could be successfully used for the selective uptake of Pb(II) from wastewater. It was also shown that the novel resins can be regenerated without significant loss of their sorption capacity.
High Purity Single Wall Carbon Nanotube by Oxygen-Containing Functional Group of Ferrocene-Derived Catalyst Precursor by Floating Catalyst Chemical Vapor Deposition
Authors: Moon Sook Young , Jeon Seung-Yeol , Lee Sung-Hyun , Lee Anna , Kim Seung Min
Published: March 2022

Single wall carbon nanotubes (SWCNTs) were synthesized using oxygen-containing ferrocene derived catalysts. The mechanism of synthesizing carbon nanotubes was clarified by the catalyst’s exothermic or endothermic decomposition processes. By monitoring the decomposition process of ferrocene-derived catalyst precursors with and without sulfur, we found that the types of oxygen function groups closely influence catalyst formation and nanotube growth. The ferrocene-derived catalyst precursors have a different oxygen containing groups, which are hydroxyl (–OH, ferrocenenemethanol) and carbonyl (C=O, acetylferrocene, and 1,1′-diacetylferrocene). The sulfur chemical state (S 2p) on synthesized catalyst particles using acetylferrocene and 1,1′-diacetylferrocene has more sulfate (SO42−) than others, and there also is a carbon state (C-S-C). The catalyst particle using ferrocenemethanol predominant formed metal–sulfur bonds (such as S2− and Sn2−). The hydroxyl group (–OH) of ferrocenemethanol enhanced the etching effect to remove amorphous carbon and prevented oxidation on the catalyst particle surfaces; however, the carbonyl group (C=O) of acetylferrocene reacted with the catalyst particles to cause partial oxidation and carbon dissociation on the surface of the catalyst particles. The partial oxidation and carbon contamination on catalyst particles controlled the activity of the catalyst. The DFT study revealed that the ferrocene-derived catalyst precursor was dissociated according to following process: the functional groups (such as CH3CO and COH) => first Cp ligands => second Cp ligands. The pyrolysis and release of Fe ions were delayed by the functional groups of ferrocene-derived precursors compared to ferrocene. The thermal-decomposition temperature of the catalyst precursor was high, the decomposition time was be delayed, affecting the formation of catalyst particles and thus making smaller catalyst particles. The size and composition of catalyst particles not only affect the nucleation of CNTs, but also affect physical properties. Therefore, the IG/ID ratio of the CNTs changed from 74 to 18 for acetylferrocene and ferrocene, respectively. The purity also increased from 79 to 90% using ferrocene-derived precursors.
Numerical Simulation of a Time-Dependent Electroviscous and Hybrid Nanofluid with Darcy-Forchheimer Effect between Squeezing Plates
Authors: Khan Muhammad Sohail , Mei Sun , Shabnam  , Fernandez-Gamiz Unai , Noeiaghdam Samad , Khan Aamir
Published: March 2022

In this article, the behavior of transient electroviscous fluid flow is investigated through squeezing plates containing hybrid nanoparticles. A hybrid nanofluid MoS2+Au/C2H6O2−H2O was formulated by dissolving the components of an inorganic substance such as molybdenum disulfide (MoS2) and gold (Au) in a base fluid of ethylene glycol/water. This hybrid non-liquid flow was modeled by various nonlinear mathematical fluid flow models and subsequently solved by numerical as well as analytical methods. For the numerical solution of nonlinear ODEs, a built-in function BVP4C was used in MATLAB, and the same problem was solved in MATHEMATICA by HAM. The result of the present problem related to the results obtained from the existing literature under certain conditions. The outcomes revealed that the concentration profiles were more sensitive to homogeneity diversity parameters. The simulation of the various physical parameters of the model indicated that the heat transfer through a mixture of hybrid nanofluids was greater than a simple nanofluid. In addition, the phenomenon of mixed convection was considered to improve the velocity of simple nanofluids and hybrid nanofluids, when both cases have low permeability. A rise in the volume fraction of the nanomaterials, Φ, was associated with an increase in the heat transfer rate. It was observed that the heat transfer rate of the hybrid nanofluids MoS2+Au/C2H6O2−H2O was higher than that of the single nanofluids MoS2/C2H6O2−H2O.
Human Polymorphonuclear Cells Support Zika Virus to Cross Endothelial Monolayer and Access Bloodstream
Authors: Gandolfo Claudia , Terrosi Chiara , Prathyumnan Shibily , Anichini Gabriele , Savellini Gianni Gori , Morgante Giuseppe , Cusi Maria Grazia
Published: March 2022

The rapid spread of new outbreaks of human infection caused by Zika virus (ZIKV) has raised many global concerns since 2016. Despite the increasing knowledge of this virus, data on the pathogenesis of ZIKV are still missing. In particular, it is still unknown how the virus crosses the endothelial monolayer and gets access to the bloodstream. In the present work, we used human umbilical vein endothelial cells (HUVECs) as a model to study ZIKV infection in vitro. We demonstrated that HUVECs are an optimal reservoir for viral replication, as they were able to sustain ZIKV infection up to two weeks, without showing a cytopathic effect. In order to evaluate the integrity of endothelial monolayer, immunofluorescence was performed on mock-infected or ZIKV-infected cells ± peripheral blood mononuclear cells (PBMCs) or polymorphonuclear cells (PMN), 48 h p.i., by using an anti-VE-Cadherin antibody, a major adherence protein that maintains the integrity of intercellular junctions. In addition to infection, we noted that the presence of some components of the immune system, such as PMNs, played an important role in altering the endothelial monolayer in cell junctions, suggesting that presence at the site of infection probably promotes the spread of ZIKV in vivo in the bloodstream.
How Much Punishment Is Deserved? Two Alternatives to Proportionality
Authors: Metz Thaddeus , Metz Mika’il
Published: March 2022

When it comes to the question of how much the state ought to punish a given offender, the standard understanding of the desert theory for centuries has been that it should give him a penalty proportionate to his offense, that is, an amount of punishment that fits the severity of his crime. In this article, we maintain that a desert theorist is not conceptually or otherwise required to hold a proportionality requirement. We show that there is logical space for at least two other, non-proportionate ways of meting out deserved penalties, and we also argue that they have important advantages relative to the dominant, proportionality approach.
Amplification and Generation of Frequency-Modulated Soliton Pulses in Nonuniform Active Fiber Configurations
Authors: Abramov Aleksei , Zolotovskii Igor , Lapin Victor , Mironov Pavel , Yavtushenko Marina , Svetukhin Vyacheslav , Fotiadi Andrei
Published: March 2022

We report on the theoretical and numerical analysis of the nonlinear Schrödinger equation describing the dynamical evolution of frequency-modulated (FM) optical signals propagating through the fiber configuration comprising active fibers with the anomalous dispersion nonuniformly distributed over the fiber length. In our consideration, a single active fiber section including segments with initially increasing and then decreasing dispersion is used for amplification and compression of an external FM pulse resulting in an increase of ~6 orders of magnitude in the pulse peak power and a 100-fold narrowing of the pulse duration down to a few picoseconds. Moreover, we demonstrate that, with a ~1 mW weakly modulated continuous wave input signal, the fiber configuration comprising two active fiber sections with different dispersion profiles is able to generate a strongly periodic pulse train, resulting in a pulse repetition rate >100 GHz, a pulse duration ~0.5 ps, and peak power up to ~1 kW. An evolution of optical signals governed by modulation instability in both fiber configurations is explored.
Cytogenetic, Morphometric, and Ecological Characterization of Festuca indigesta Boiss. in the Southeast of Spain
Authors: Martínez-Sagarra Gloria , Casimiro-Soriguer Federico , Castro Sílvia , Loureiro João , Devesa Juan A.
Published: March 2022

Festuca indigesta subsp. indigesta (Poaceae) is endemic to the southeast of Spain, and until recently, it was considered that its range of distribution was restricted to the siliceous core of Sierra Nevada. However, it has been recently extended in the territory to others calcareous mountains. This study investigates the cytogenetic variability throughout the geographic range of this taxon, the possible edaphic preferences of each cytotype, and the morphological variation of cytotypes. Genome sizes and ploidy levels were estimated using flow cytometry and chromosome count. Soil samples were collected to test the nature of the substrate, i.e., pH, and calcium and magnesium contents. Finally, morphological characters were measured in herbarium specimens. This study provides the first genome size data for the species. Hidden cytogenetic diversity was detected in the taxon, comprising hexaploid (2n = 6x = 42), octoploid (2n = 8x = 56) and dodecaploid (2n = 12x = 84) individuals. No relationship between substrate nature and cytotype was observed. Morphological differences were detected for the size of floral parts and stomata among cytotypes, but these were blurred if the entire morphological variation range was considered. Our results suggest that each mountain range could act as a reservoir of morphologically cryptic genetic diversity regarding this taxon.
Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map
Authors: Alahakoon Dilmini , Fennell Anne , Helget Zachary , Bates Terry , Karn Avinash , Manns David , Mansfield Anna Katharine , Reisch Bruce I. , Sacks Gavin , Sun Qi , Zou Cheng , Cadle-Davidson Lance , Londo Jason P.
Published: March 2022

Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F2 population (VRS-F2) that was derived by selfing a single F1 from a Vitis riparia x ‘Seyval blanc’ cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the Vitis vinifera ‘PN40024’ genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq transferable markers were identified that can be used in pairs (one pistillate and one hermaphroditic) to predict pistillate and hermaphrodite flower type with ≥99.7% accuracy. Total and individual anthocyanin diglucoside QTL mapped to chromosome 9 near a 5-O-GLUCOSYLTRANSFERASE candidate gene. Malic acid QTL were observed on chromosome 1 and 6 with two MALATE DEHYRDROGENASE CYTOPLASMIC 1 and ALUMINUM-ACTIVATED MALATE TRANSPORTER 2-LIKE (ALMT) candidate genes, respectively. Modeling malic acid identified a potential QTL on chromosome 8 with peak position in proximity of another ALMT. A first-ever reported QTL for the grassy smelling volatile (E)-2-hexenal was found on chromosome 2 with a PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE candidate gene near peak markers.
Quality Control Standardization, Contaminant Detection and In Vitro Antioxidant Activity of Prunus domestica Linn. Fruit
Authors: Amir Mohd , Zafar Ameeduzzafar , Ahmad Rizwan , Ahmad Wasim , Sarafroz Mohammad , Khalid Mohammad , Ghoneim Mohammed M. , Alshehri Sultan , Wahab Shadma , Ahmad Sayeed , Mujeeb Mohd
Published: March 2022

The increase in the use of herbal medicines has led to the implementation of more stern regulations in terms of quality variation and standardization. As medicinal plants are prone to quality variation acquired due to differences in geographical origin, collection, storage, and processing, it is essential to ensure the quality, efficacy, and biological activity of medicinal plants. This study aims to standardize the widely used fruit, i.e., Prunus domestica Linn., using evaluation techniques (microscopic, macroscopic, and physicochemical analyses), advanced instrumental (HPLC, HPTLC, and GC–MS for phytochemical, aflatoxins, pesticides, and heavy metals), biological, and toxicological techniques (microbial load and antioxidant activities). The results revealed a 6–8 cm fruit with smooth surface, delicious odor, and acidic taste (macroscopy), thin-walled epidermis devoid of cuticle and any kind of excrescences with the existence of xylem and phloem (microscopy), LOD (15.46 ± 2.24%), moisture content (13.27 ± 1.75%), the high extractive value of 24.71 ± 4.94% in water:methanol (1:1; v/v) and with ash values in the allowed limits (physicochemical properties), and the presence of numerous phytochemical classes such as alkaloids, flavonoids, carbohydrates, glycosides, saponins, etc. (phytochemical screening). Furthermore, no heavy metals (Pb, Hg, Cd, Ar), pesticides, ad microbial limits were detected beyond the permissible limits specified, as determined with AAS, GC–MS analysis, and microbial tests. The HPTLC was developed to characterize a complete phytochemical behavior for the components present in P. domestica fruit extract. The parameters utilized with the method used and the results observed for the prunus herein may render this method an effective tool for quality evaluation, standardization, and quality control of P. domestica fruit in research, industries, and market available food products of prunus.
Investigating the Effect of Low-Temperature Drilling Process on the Mechanical Behavior of CFRP
Authors: Wang Hongxiao , Zhang Xiaohui , Duan Yugang
Published: March 2022

Previous research has found that lower temperature drilling is helpful to improve the hole quality of carbon fiber reinforced polymer (CFRP). However, the influence of the lower temperature drilling process on the mechanical behavior of composites is yet not fully understood. To examine the influence of the lower temperature drilling process on the mechanical behavior of CFRP, the open hole CFRP specimens used for mechanical tests were obtained with three cases: drilling with −25 °C/uncoated carbide drills/(1000 rpm, 0.02 mm/r), 23 °C/coated carbide drills/(4000 rpm, 0.03 mm/r), and 23 °C/uncoated carbide drills/(1000 rpm, 0.02 mm/r), respectively; corresponding, three groups of open-hole specimens are obtained: specimens drilling at low-temperature with low damage, specimens drilling at room-temperature with low damage and specimens drilling at room-temperature with low damage; the mechanical behavior of the three groups specimens were obtained by static tensile, tensile–tensile fatigue cyclic tests and residual tensile strength test. The results have shown that the mechanical properties of specimens with a low-temperature drilling process is lower than those of the specimen with a normal drilling process due to the better drilling quality. The damage accumulation in specimens was increased with the damage degree of the original hole, the greater the damage degree, the worse the mechanical properties.
Calibration and Testing of Discrete Element Simulation Parameters for Urea Particles
Authors: Bu Haoran , Yu Siyao , Dong Wancheng , Wang Yuqi , Zhang Lixin , Xia Yuanqing
Published: March 2022

The lack of accurate simulation model parameters in the optimization design process of variable fertilizer application devices has resulted in large errors between simulation and theoretical calculation results, which has restricted the development of variable fertilizer application devices to a certain extent. Additionally, there are few scholars studying urea granules, so many parameters of urea granular fertilizer cannot be directly obtained from the literature. The aim of this study is to calibrate a set of simulation parameters by combining physical and simulation tests. In this study, intrinsic parameters were systematically determined, including the particle size, particle density, elastic modulus, Poisson’s ratio and their static friction coefficients, rolling friction coefficients and restitution coefficients of urea particles. By performing the urea particle stacking test, the static friction coefficient between urea particles was calibrated to 0.27, and the rolling friction coefficient between particles was 0.11. To check the reliability of the calibration parameters, the simulation and physical tests of the repose angle and bulk density of urea particles were compared, and the results show that the relative error of repose angles and bulk density of urea particles was 0.78% and 1.19%, respectively. Through the simulation of the mechanical variable fertilizer discharger and the comparison test of the benchtop fertilizer discharging performance, the maximum relative error between the simulation and physical test fertilizer discharge is 3.69% when the working length of the outer sheave is 25 mm; the maximum relative error between the simulation and physical test fertilizer discharge is 3.39% when the working length is 35 mm; the maximum relative error between the simulation and physical test fertilizer discharge is 6.86% when the working length is 45 mm; the maximum relative error between the simulation and physical test fertilizer discharge is 4.95% when the working length is 55 mm. The maximum relative error between the simulated and physical test fertilizer discharge was 6.86% at 45 mm opening and 4.95% at 55 mm opening, and the results show that the urea particle calibration parameters are reliable. The results of this study can provide a theoretical reference for the optimization design and simulation study of variable fertilizer application devices.
Application Research of Soft Computing Based on Machine Learning Production Scheduling
Authors: Fülöp Melinda Timea , Gubán Miklós , Gubán Ákos , Avornicului Mihály
Published: March 2022

An efficient and flexible production system can contribute to production solutions. These advantages of flexibility and efficiency are a benefit for small series productions or for individual articles. The aim of this research was to produce a genetic production system schedule similar to the sustainable production scheduling problem of a discrete product assembly plant, with more heterogeneous production lines, and controlled by one-time orders. First, we present a detailed mathematical model of the system under investigation. Then, we present the IT for a solution based on a soft calculation method. In connection with this model, a computer application was created that analyzed various versions of the model with several practical problems. The applicability of the method was analyzed with software specifically developed for this algorithm and was demonstrated on a practical example. The model handles the different products within an order, as well as their different versions. These were also considered in the solution. The solution of this model is applicable in practice, and offers solutions to better optimize production and reduce the costs of production and logistics. The developed software can not only be used for flexible production lines, but also for other problems in the supply chain that can be employed more widely (such as the problem of delivery scheduling) to which the elements of this model can be applied.
Influence of External Natural Environment Including Sunshine Exposure on Public Mental Health: A Systematic Review
Authors: Taniguchi Keita , Takano Mayuko , Tobari Yui , Hayano Motoshi , Nakajima Shinichiro , Mimura Masaru , Tsubota Kazuo , Noda Yoshihiro
Published: March 2022

The COVID-19 pandemic has been raging around the world and public health measures such as lockdowns have forced people to go out less often, reducing sunlight exposure time, green space use, and physical activity. It is well known that exercise has a positive impact on mental health, but the impact of external environmental factors such as sunlight exposure and green space use on mental health has not been systematically reviewed. In this review, we categorized the major factors that may affect people’s mental health into (1) external environmental factors such as exposure to sunlight and green spaces, (2) internal life factors such as physical activity and lifestyle, and (3) mixed external and internal factors, and systematically examined the relationship between each factor and people’s mental health. The results showed that exposure to sunlight, spending leisure time in green spaces, and physical activity each had a positive impact on people’s mental health, including depression, anxiety, and stress states. Specifically, moderate physical activity in an external environment with sunlight exposure or green space was found to be an important factor. The study found that exposure to the natural environment through sunbathing and exercise is important for people’s mental health.
The Reception of Jeremiah in Modern Hebrew Literature
Authors: Avioz Michael
Published: March 2022

Looking at some illustrative examples of the reception of Jeremiah in modern Hebrew literature, this article explores how both the prophet and the book named after him were reworked by modern Hebrew authors and poets in the body of literary works in Hebrew that emerged during the late eighteenth and early nineteenth centuries in Europe in the wake of the Enlightenment.
Deep-Learning-Based Parking Area and Collision Risk Area Detection Using AVM in Autonomous Parking Situation
Authors: Lee Sunwoo , Lee Dongkyu , Kee Seok-Cheol
Published: March 2022

In this paper, I propose a bird eye view image detection method for parking areas and collision risk areas at the same time in parking situations. Deep learning algorithms using area detection and semantic segmentation were used. The main architecture of the method described in this paper is based on a harmonic densely connected network and a cross-stage partial network. The dataset used for training was calibrated to four 190° wide-angle cameras to generate around view monitor (AVM) images based on the Chungbuk National University parking lot, and an experiment was performed based on this dataset. In the experimental results, the available parking area was visualized by detecting the parking line, parking area, and available driving area in the AVM images. Furthermore, the undetected area in the semantic segmentation as a collision risk area was visualized in order to obtain the results. According to the proposed attention CSPHarDNet model, the experimental results were 81.89% mIoU and 18.36 FPS in a NVIDIA Xavier environment. The results of this experiment demonstrated that algorithms can be used in real time in a parking situation and have better performance results compared to the conventional HarDNet.
Real-Time Internet of Medical Things System for Detecting Blood Leakage during Hemodialysis Using a Novel Multiple Concentric Ring Sensor
Authors: Hu Hsiang-Wei , Liu Chih-Hao , Du Yi-Chun , Chen Kuan-Yu , Lin Hsuan-Ming , Lin Chou-Ching
Published: March 2022

Venous needle dislodgement (VND) is a major healthcare safety concern in patients undergoing hemodialysis. Although VND is uncommon, it can be life-threatening. The main objective of this study was to implement a real-time multi-bed monitoring system for VND by combining a novel leakage-detection device and IoMT (Internet of Medical Things) technology. The core of the system, the Acusense IoMT platform, consisted of a novel leakage-detection patch comprised of multiple concentric rings to detect blood leakage and quantify the leaked volume. The performance of the leakage-detection system was evaluated on a prosthetic arm and clinical study. Patients with a high risk of blood leakage were recruited as candidates. The system was set up in a hospital, and the subjects were monitored for 2 months. During the pre-clinical simulation experiment, the system could detect blood leakage volumes from 0.3 to 0.9 mL. During the test of the IoMT system, the overall success rate of tests was 100%, with no lost data packets. A total of 701 dialysis sessions were analyzed, and the accuracy and sensitivity were 99.7% and 90.9%, respectively. Evaluation questionnaires showed that the use of the system after training changed attitudes and reduced worry of the nursing staff. Our results show the feasibility of using a novel detector combined with an IoMT system to automatically monitor multi-bed blood leakage. The innovative concentric-circle design could more precisely control the warning blood-leakage threshold in any direction to achieve clinical cost-effectiveness. The system reduced the load on medical staff and improved patient safety. In the future, it could also be applied to home hemodialysis for telemedicine during the era of COVID-19.
Noise-Resilient Depth Estimation for Light Field Images Using Focal Stack and FFT Analysis
Authors: Sharma Rishabh , Perry Stuart , Cheng Eva
Published: March 2022

Depth estimation for light field images is essential for applications such as light field image compression, reconstructing perspective views and 3D reconstruction. Previous depth map estimation approaches do not capture sharp transitions around object boundaries due to occlusions, making many of the current approaches unreliable at depth discontinuities. This is especially the case for light field images because the pixels do not exhibit photo-consistency in the presence of occlusions. In this paper, we propose an algorithm to estimate the depth map for light field images using depth from defocus. Our approach uses a small patch size of pixels in each focal stack image for comparing defocus cues, allowing the algorithm to generate sharper depth boundaries. Then, in contrast to existing approaches that use defocus cues for depth estimation, we use frequency domain analysis image similarity checking to generate the depth map. Processing in the frequency domain reduces the individual pixel errors that occur while directly comparing RGB images, making the algorithm more resilient to noise. The algorithm has been evaluated on both a synthetic image dataset and real-world images in the JPEG dataset. Experimental results demonstrate that our proposed algorithm outperforms state-of-the-art depth estimation techniques for light field images, particularly in case of noisy images.