bluuebunny/crossref_metadata_2025 huggingface dataset:
A TRANSFORMAÇÃO DA PRÁTICA JUDICIÁRIA COM A IMPLANTAÇÃO DO PROCESSO ELETRÔNICO
Authors: Lopes Ribeiro Graziele , Pereira Wanderley Kauã
Published: September 2020

O congestionamento de processos sobrecarrega o Poder Judiciário brasileiro e interfere diretamente na qualidade e eficiência da prestação jurisdicional à sociedade. Diante deste cenário, surgiu uma ferramenta tecnológica cujo objetivo é a celeridade processual. A digitalizaram dos processos e migração para o sistema e-proc minimizaram o congestionamento judicial. O Tocantins foi o primeiro estado brasileiro a ter 100% dos processos digitalizados e desde então a taxa de congestionamento tem diminuído e a de produtividade aumentado. O presente trabalho pretende verificar se a ferramenta do e-proc propicia a celeridade processual e eficiência na prática de prestação de serviços aos cidadãos e na prática judiciária para os operadores do Direito no Tribunal de Justiça do Estado do Tocantins, através da análise comparativa do sistema e-proc nas varas criminais do Tribunal de Justiça do Estado do Tocantins (TJTO) no período de 2011 a 2016. O caminho metodológico consistiu numa pesquisa documental com analise de relatórios de gestão do Tribunal de Justiça do Tocantins. Concluiu-se que, houve uma diminuição na taxa de congestionamento de processos após a implantação de e-proc e diversas outras vantagens que acarretam na melhoria da prestação jurisdicional.
OS DIREITOS SOCIAIS E A CONCILIAÇÃO COMO MECANISMO DE RESOLUÇÃO DE CONFLITOS
Authors: de Lima Geist Cristiane
Published: September 2020

O presente artigo versa sobre os direitos sociais e a conciliação como mecanismo para enfrentar a crise produzida pelo excesso de judicialização do Estado de Bem-Estar Social. A conciliação visa imprimir celeridade na solução dos conflitos, diminuição de custos e desgastes. Por meio do método de abordagem hipotético-dedutivo e do método de procedimento histórico, monográfico e dissertativo, com a utilização de pesquisas bibliográficas, a investigação percorreu os seguintes caminhos: partiu do estudo do surgimento dos direitos sociais, as dificuldades do Estado na implementação de tais direitos. Posteriormente dando especial enfoque a conciliação tomada como mecanismo redutor de custos e desgastes entre as relações sociais e estatais, semeadora da cultura da paz e incentivadora do exercício da cidadania. Busca-se, assim, indicar um possível patamar superior das relações entre os privados, produzido a partir do exemplo do Estado na relação com a sociedade, encurtando o caminho de acesso aos direitos, diminuindo gastos públicos e privados, fomentando o exercício democrático da cidadania e a cultura da paz, tudo no intuito de dar eficácia aos objetivos do Estado de Bem-Estar Social e evitar o risco das tentações populistas e/ou autoritárias, esse monstro nem tão adormecido a fustigar as sociedades em crise.
Pediatric Inflammatory Multisystem Syndrome (PIMS) Did Occur in Poland during Months with Low COVID-19 Prevalence. Preliminary Results of a Nationwide Register.
Authors: Okarska-Napierała Magdalena , Ludwikowska Kamila , Szenborn Leszek , Dudek Natalia , Mania Anna , Buda Piotr , Książyk Janusz , Mazur-Malewska Katarzyna , Figlerowicz Magdalena , Szczukocki Maciej , Kucińska Beata , Werner Bożena , Stopyra Lidia , Czech Agnieszka , Berdej-Szczot Elżbieta , Gawlik Aneta , Opalińska Paulina , Mazur Artur , Januszkiewicz-Lewandowska Danuta , Niszczota Cezary , Jackowska Teresa , Wysocki Jacek , Tracewski Paweł , Kusa Jacek , Potocka Paulina , Sulik Artur , Ziora Katarzyna , Krakowczyk Helena , Wodniak Aleksandra , Zaryczański Janusz , Żakiewicz-Cepak Alicja , Jarocka-Cyrta Elżbieta , Koczwara Agnieszka , Prusek-Dudkiewicz Anna , Zbucka-Jachowska Ewa , Langer-Wójcik Sabina , Kołodziej Elżbieta , Kuchar Ernest
Published: September 2020

Pediatric inflammatory multisystem syndrome (PIMS) is a new entity in children, likely associated with previous coronavirus disease 19 (COVID-19). Most of reports about PIMS come from countries particularly hit by the COVID-19 pandemic. Our aim was to investigate the nature of inflammatory syndromes in Poland (a country with low COVID-19 prevalence) and to perceive the emergence of PIMS in our country. On May 25th, we have launched a nationwide survey of inflammatory syndromes in children for retrospective (since 4th March 2020) and prospective data collection. Up to 28th July, 39 reported children met inclusion criteria. We stratified them according to age (<5 and ≥ 5 years old) and COVID-19 status. The majority of children had clinical and laboratory features of Kawasaki disease, probably non-associated with COVID-19. However, children ≥5 years of age had PIMS characteristics, and 9 children had COVID-19 confirmation. This is the first to our knowledge report of PIMS register from the country with low COVID-19 prevalence, and it proves that PIMS may emerge in any area involved in the COVID-19 pandemic. In a context of limited COVID-19 testing availability, other risk factors of PIMS, e.g. older age should be considered in the differential diagnosis of inflammatory syndromes in children.
Konstruksi Citra Joko Widodo dalam Sampul Majalah Milenial Arrajol, Arab Saudi, Edisi 20 Mei 2019
Authors: Hendro Eko Setiawan
Published: September 2020

Indonesian President Joko Widodo is widely discussed on social media because he was chosen as the cover story in Arrajol, a monthly Arabic men’s lifestyle magazine published in Saudi Arabia. On its official Facebook page, Arrajol displays a promotional video of their latest edition by writing, “President of Indonesia, a figure in the new edition of Arrajol magazine.” The video was uploaded by Arrajol on Thursday, May 16, 2019. The purposes of this study are to determine the symbol contained in Arrajol, the meaning contained within the symbol, and how these symbols constructing Joko Widodo’s image as a role model. This study uses qualitative methods with semiotics analysis from Charles Sanders Peirce. The result of this study showed Arrajol magazine consists of two main symbols, the gesture symbol of Joko Widodo who is dressed in a suit with a thin smile on his face, is Joko Widodo’s authority, and followed by the text on the bottom right showing Joko Widodo’s personality. These symbols have different meanings. The meaning contained in each symbol constructs the image of Joko Widodo as a model for the world community.
DAB2IP predicts treatment response and prognosis of ESCC patients and modulates its radiosensitivity through enhancing IR-induced activation of the ASK1-JNK pathway
Authors: Li Yue , Xu Meng , Fang Weiyang , Sheng Lei , Chen Xiangcun , Xu Jifei , Zha Tianqi , Xiao Liang , Pan Shuhao , Pan Huaguang , Chai Huiping , Zhao Lei , Wang Hao , Xie Dan , Tong Zhuting
Published: September 2020

BACKGROUND: Disabled homolog 2 interacting protein (DAB2IP) plays a tumor-suppressive role in several types of human cancers. However, the molecular status and function of the DAB2IP gene in esophageal squamous cell carcinoma (ESCC) is rarely reported. METHODS: We examined the expression dynamics of DAB2IP by immunohistochemistry (IHC) in 140 ESCC patients treated with definite chemoradiotherapy. A series of in vivo and in vitro experiments were performed to elucidate the effect of DAB2IP on the chemoradiotherapy (CRT) response and its underlying mechanisms in ESCC.RESULTS: Decreased expression of DAB2IP in ESCCs correlated positively with ESCC resistance to CRT and was a strong and independent predictor for short disease-specific survival (DSS) of ESCC patients. Furthermore, the therapeutic sensitivity of CRT was substantially increased by ectopic overexpression of DAB2IP in ESCC cells. In addition, knockdown of DAB2IP dramatically enhanced resistance to CRT in ESCC. Finally, we demonstrated that DAB2IP regulates ESCC cell radiosensitivity through enhancing ionizing radiation (IR)-induced activation of the ASK1-JNK signaling pathway.CONCLUSIONS: Our data highlight the molecular etiology and clinical significance of DAB2IP in ESCC, which may represent a new therapeutic strategy to improve therapy and survival for ESCC patients.
The Effect of Various Cognitive-Motor and Motor-Motor Tasks on the Motor and Cognitive Function of Patients Multiple Sclerosis: A Randomized Controlled Trial
Authors: Abasnia Arash , Shahbazi Mehdi , Ameri Elahe Arab
Published: September 2020

Background: 85% of multiple sclerosis patients suffer from impaired motor function and 65% from cognitive impairment. Therefore The main purpose of this study is to improve the cognitive and motor function of patients using dual tasks.Methods: The study samples were 60 women with MS (20-50 years) with an Expanded Disability Status Scale 2-4 and the duration of the period (10-14 years) who were divided into 4 groups and for eight weeks each week including two sessions 45 They practiced dual cognitive, motor, and individual training for 45 to 60 minutes. Data were analyzed by composite variance analysis at significance level of P <0.05.Results: The results of compound analysis analysis showed that the training of cognitive-motor tasks improved the significance of motor function (p = 0.0005) and cognitive performance (p = 0.0001) compared to the other three groups.Conclusion: The results of this study suggest that the physical benefits of training are always superior to cognitive changes. Dual-task training can be considered a good way to improve cognitive and cognitive function.Trial registration: This study was registered in the Iranian Clinical Trial Center with the number IRCT20200812048384N1, http://www.irct.ir/trial/50300, registered 26 August 2020.
Clinical Application of Confidence Interval for Monitoring Changes in Tumor Markers to Determine the Responsiveness to Cancer Treatment
Authors: Cho Jooyoung , Seo Dong Min , Uh Young
Published: September 2020

Background: Tumor markers are used to monitor disease progression and determine the responsiveness to cancer treatment. However, there are no standardized criteria for monitoring serial tumor marker measurements. Herein, we have developed our own monitoring system for interpreting changes in tumor markers using overlapping 95% confidence intervals (CIs) to determine whether the changes are significant.Methods: Two-year data, including 117,289 results for 11 tumor markers in our laboratory, were analyzed. The distributions of absolutely delta% and cut-off values for certain percentiles were calculated. CI ranges for each tumor marker were set based on biological variation, and data were analyzed for each patient assessed at health check-ups and clinics, individually and overall.Results: Most tumor markers had low indices of individuality, with between inter-individual variability. The 95th percentile cut-offs for each tumor marker were much higher in the health check-up group than in the clinic group. In decreasing order, the percentages of results with no overlap in 95% CIs were thyroglobulin antigen, 14.9%; protein induced by vitamin K absence-II (PIVKA), 11.9%; prostate-specific antigen, 9.8%; and cancer antigen 72-4, 8.7%. After correction using the reference interval, the percentages decreased to less than 5%, except for PIVKA (10.9%).Conclusions: We suggest that our own monitoring system can serve as a criterion for delta check and auto-verification of tumor markers. Further studies are required to validate and demonstrate this concept in real clinical situations using actual clinical data reflecting disease progression in cancer patients and responsiveness to cancer treatment.
DNA methylation in Verticillium dahliae requires only one of three putative DNA methyltransferases, yet is dispensable for growth, development and virulence
Authors: Kramer Martin , Cook David , Berg Grardy van den , Seidl Michael , Thomma Bart
Published: September 2020

Background: DNA methylation is an important epigenetic control mechanism that in many fungi is restricted to genomic regions containing transposons. Two DNA methyltransferases, Dim2 and Dnmt5, are known to perform methylation at cytosines in fungi. While most ascomycete fungi encode both Dim2 and Dnmt5, only few functional studies have been performed in species containing both. Methods: In this study, we report functional analysis of both Dim2 and Dnmt5 in the plant pathogenic fungus Verticillium dahliae. Results: Our results show that Dim2, but not Dnmt5 or the putative sexual-cycle related DNA methyltransferase Rid, is responsible for nearly all DNA methylation. Single or double DNA methyltransferase mutants did not show altered development, virulence, or transcription of genes or transposons. In contrast, Hp1 and Dim5 mutants that are impacted in chromatin-associated processes upstream of DNA methylation are severely affected in development and virulence and display extensive transcriptional reprogramming in specific hypervariable genomic regions (so-called lineage-specific (LS) regions) that contain genes associated with host colonization. As these LS regions are largely devoid of DNA methylation and of Hp1- and Dim5-associated heterochromatin, the differential transcription is likely caused by pleiotropic effects rather than by differential DNA methylation. Conclusion: Overall, our study suggests that Dim2 is the main DNA methyltransferase in V. dahliae and, in conjunction with work on other fungi, is likely the main active DNMT in ascomycetes, irrespective of Dnmt5 presence. We speculate that Dnmt5 acts under specific, presently enigmatic, conditions or, alternatively, acts in DNA-associated processes other than DNA methylation.
Development of Chinese chestnut whiskey: yeast strains isolation, fermentation system optimization, and scale-up fermentation
Authors: Shoubao Yan , Qianqian Wu , Jiaquan Guang , CuiE Shi
Published: September 2020

In this study, we used Chinese chestnut as the main raw material to develop a novel type of whiskey. First, 16 yeasts were isolated and identified for producing aroma using olfactory plate assay. Of these, we screened nine yeast strains based on their fermentation capacity, aroma profile, and sensory evaluation. The results demonstrated the combination of strains HN006 (Saccharomyces cerevisiae) and HN010 (Wickerhamomyces anomalus) provided satisfactory wine fermentation with an interesting flavor profile, as strain HN010 was highly aromatic and had elevated sensory scores with comparatively low ethanol yield, while strain HN006 had a poor flavor profile but produced the largest amount of ethanol. Subsequently, we co-cultured strains HN006 and HN010 to optimize the fermentation system. The results revealed the following optimum parameters: a mixed inoculum of 6% (v/v) at an HN006/HN010 ratio of 1: 2 (v/v), a raw material ratio of 5: 3: 2 (chestnut: malt: glutinous rice), and yeast extract concentration of 6 g/L. Additionally, this fermentation system was successfully scaled-up to a 1000 L pilot-scale system. The results of this study showed that strains HN006 and HN010 could be used as alternatives for whiskey fermentation, as well as provided a generalized experimental scheme to assess other microorganisms.
The Characteristics of Patients with Subsequent Distal Radius Fracture After Initial Distal Radius Fracture
Authors: Lee Jongjin , Kim Jae Kwang , Oh Minyoung , Shin Young Ho
Published: September 2020

Background: The characteristics of patients who experienced subsequent distal radius fracture (DRF) after initial DRF have not been determined. The purpose of this study was to investigate the characteristics of patients with subsequent DRF and to compare bone fragility parameters of patients with primary and subsequent distal DRF. Methods: We compared demographic characteristics and bone fragility parameters, including bone mineral densities (BMDs), trabecular bone score (TBS), hip geometry parameters, and bicortical thickness (BCT) of the distal radius in 215 patients with initial DRF and 26 patients with subsequent DRF. To reduce bias, patients with subsequent DRF were propensity score-matched in a 1:2 manner with patients affected by initial DRF, and additional comparison was performed.Results: Patients in the subsequent DRF group were older than those in the initial DRF group, but this difference was not significant (p = 0.091). The proportion of patients receiving osteoporosis medications was significantly higher in the subsequent DRF group (41.7% vs. 19.2%, p = 0.011). Bone fragility parameters, including BMD, TBS, hip geometry parameters, and BCT did not differ significantly between the two groups. However, ten-year probability of major osteoporotic fractures was significantly higher in patients with subsequent DRF (p < 0.001). Similar results were observed upon comparing the propensity score-matched initial and subsequent DRF groups.Conclusions: These findings suggest that the occurrence of subsequent DRF after initial DRF can be attributed to multiple factors rather than bone fragility alone. Systematic and multidisciplinary managements would be helpful for preventing the occurrence of subsequent DRF after initial DRF.
Metastatic Ovariectomy is Beneficial for Survival in Ovarian Metastasis of Colorectal Carcinoma: A Single-center Experience in China
Authors: Jin Min , Wang Lanqing , Luo Lihua , Zhang Dejun , Zhao Lei , Xu Guojie , Hu Jianli , Zhang Tao
Published: September 2020

BackgroundThe benefit of ovarian resection in cases of ovarian metastases from colorectal carcinoma (CRC) is still uncertain. The purpose of this study is to explore the benefits of metastatic ovariectomy plus chemo over chemo alone in the therapy of ovarian metastasis of CRC and to ascertain prognostic factors. MethodsA retrospective analysis of 54 female patients with ovarian metastasis of CRC was performed in a single-center between December 2009 and December 2017 in China. The subjects were divided into two subgroups: metastatic ovariectomy plus chemo subgroup and chemo alone subgroup. The clinicopathological features and overall survival (OS) were analyzed between the two subsets. Univariate and multivariate analysis were further performed to identify the risk factors for OS in these patients.ResultsThe clinicopathological characteristics between the two subsets were similar, including 34 patients (63%) with ovariectomy plus chemo and 20 patients (37%) with chemo alone. In the total of 54 patients, the median survival time was 19.0 months (95% confidence interval, 5.0 to 60.0 months). The median survival time of metastatic tumor resection plus chemo subgroup was 27.9 months (95% confidence interval, 20.8 to 35.0 months), which was significantly better than that of simple chemo subgroup, 16.4 months (95% confidence interval, 11.8 to 21.0 months) (P < 0.01). When stratified analysis of the ovariectomy value in different time phases of ovarian metastasis, it indicated that ovariectomy plus chemo had superior survival benefits than chemo alone in CRC patients with metachronous metastasis (34.8 months vs. 18.4 months; P < 0.05) but not synchronous metastasis (17.2 months vs.13.3months; P =0.29). ConclusionOur results indicate that the removal of ovarian metastases of CRC can improve survival, especially in metachronous metastasis subjects. This study provides a support for ovariectomy of ovarian metastasis from CRC in clinical treatment for oncologist.Running tittleTreatment Strategy and Prognostic Analysis of Ovarian Metastasis of Colorectal Carcinoma
Phoenixin 20 Prevents ox-LDL-Induced Attachment of Monocytes to Human Aortic Endothelial cells (HAECs): A Protective Implication in Atherosclerosis
Authors: Lin Hua-Song , Wei Xiao-Lan , Zheng Bi-Rong , Lin Ruo-Ting , Huang Ya-li , Li Mi-Mi , Chen Ya-Fang , Zhang Jin-Ying , Li Wei-wei , Ye Lichao
Published: September 2020

Background: The cause of atherosclerosis is not known, and therefore the current treatment options are limited. In the present study, we aimed to investigate the effects of Phoenixin 20 and its receptor G protein-coupled receptor 173 (GPR173) against ox-LDL- induced endothelial dysfunction. Materials and Methods: Human aortic endothelial cells (HAECs) were treated with 10 μg/ml ox-LDL in the presence or absence of phoenixin 20. Gene expression of GPR173, ICAM-1, VCAM-1, IL-1β, IL-8, MCP-1, and NOX-4 were measured by real time PCR. Protein expression was assayed by western blot analysis. Secretions of pro-inflammatory cytokines were measured by ELISA. The attachment of THP-1 monocytes to HAECs was detected using calcein-AM staining. Transcriptional activity of NF-κB was measured using dual-luciferase reporter assay. Results: Our findings indicate that ox-LDL significantly lowered the expression of GPR173 in HAECs and triggered an increase in ROS, NOX-4, and proinflammatory cytokine expression. Importantly, we demonstrate that agonism of GPR173 using phoenixin 20 significantly ameliorated these harmful effects of ox-LDL. We also show that agonism of GPR173 can prevent the attachment of monocytes to endothelial cells, which is an important therapeutic approach to prevent atherogenesis. Conclusion: Here, for the first time to our knowledge, we provide a basis for future research on the role of GPR173 as a new potential treatment against atherosclerosis.
Prevalence of musculoskeletal disorders among perioperative nurses: a systematic review and meta-analysis
Authors: Clari Marco , Godono Alessandro , Garzaro Giacomo , Voglino Gianluca , Gualano Maria Rosaria , Migliaretti Giuseppe , Gullino Attilia , Ciocan Catalina , Dimonte Valerio
Published: September 2020

Background
To evaluate the prevalence of work-related musculoskeletal disorders (WRMSDs) in perioperative nurses and to explore their association with personal characteristics.
Methods
Medline, Cumulative Index to Nursing and Allied Health Literature, Scopus, Web of Science, Cochrane Library and Joanna Briggs Institute Database were systematically searched. A meta-analysis calculating event rates, and relative 95% Confidence Intervals (CI) was performed for each musculoskeletal district. The contribution of perioperative nurses’ gender, age, and BMI was assessed trough a meta-regression.
Results
Twenty-two studies, considering 3405 perioperative nurses, were included in the systematic review. The highest prevalence of WRMSDs was found for the lower-back (61%; 95% CI 0.52–0.70), followed by shoulder (45%; 95% CI 0.38–0.53), waist (45%; 95% CI 0.34–0.57), knee (45%; 95% CI 0.32–0.54), neck (39%; 95% CI 0.27–0.52), upper-back (34%; 95% CI 0.24–0.46), ankle-feet (33%; 95% CI 0.18–0.51), hand-wrist (29%; 95% CI 0.19–0.41), and elbow (18%; 95% CI 0.11–0.27). Meta-regression showed that gender, age, and BMI were not significant predictors of low-back disorders (p = 0.69; R2 = 0).
Conclusions
WRMSDs represent a high prevalence issue among perioperative nurses. Perioperative nurses, in general, are steadily exposed to both physical and temporal risk factors. Several interventions may be adopted to reduce the burden of WRMSDs, including ergonomic education and physical rehabilitation. Our data could be used in future studies as a reference to assess the risk of WRMSDs in other health-care professionals’ population.
Structure, Morphological, and Physicochemical Characteristics of a New Composite Coagulant Made From Polyaluminium Chloride and Agro-waste of Tapioca Peel
Authors: SNA Mohd-Salleh , MZN Shaylinda , N Othman , G Yashni , AB Norshila , MS Sainudin
Published: September 2020

A combination of a metal coagulant with an organic polymer can complement both the outstanding and flaw properties of the separate elements. A novel composite coagulant of polyaluminium chloride (PAC)-tapioca peel extract powder (TPP) called PACTPP with different weights ratio was prepared in this study. In the preliminary study, PACTPPg at the weight/weight ratio of TPP/Al=3.71 was selected as the optimum one based on the performance in treating wastewater sample of landfill leachate. Through all characterisation analyses, it revealed that the novel composite reagent exhibits better coagulant properties when compared with the individual coagulants of PAC and TPP. It was characterised that PACTPPg had combined the best benefits from PAC and TPP, with an acidic property of pH 3.45, the low charge density of 3.45 mV, the higher molecular weight of 1.59 × 107 g/mol, the bigger particle size of 4.528 × 104 d.mn, and a longer connected and compact structure. Through Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analyses, PACTPPg was identified to comprise new chemical compounds, i.e., the functional groups of ketones, aldehydes, and alkanes in a semi-formed crystalline phase. Based on the comparison study, it can be concluded that PACTPPg showed a correlation to encompass the complex interpenetration networks rather than just a simple mechanical mixing of raw materials.
Chemokine Receptor 7 (CCR7) Participates in the Migration of DCs and Has Impact on the Progression of Primary Pterygium
Authors: Wang Ting , Wang Tao , Ni Huanqi , Zhong Lei , Cheng Huanhuan , Bao Tiancheng , Jia Xiuhua , Xia Jun , Liu Xinyu , Lin Haotian , Ling Shiqi
Published: September 2020

Background: The effect of chemokine receptors and their ligands for dendritic cells (DCs) plays a critical role in the immune response, but whether chemokine receptor 7 (CCR7) has an impact on DCs migrating in pterygia remains unclear. The aim of this article is to investigate the involvement of CCR7 signaling in pathogenesis of primary pterygium.Methods: Slip lamp photographs of 85 pterygia patients were used to divide the pterygia into three groups, the width, extension, and area of pterygia were measured by computer software, the blood vessels of pterygium and general eye redness were quantitatively analyzed by a specific algorithm. Expression of CCR7 and its ligands C-C motif ligand 19 (CCL19) and C-C motif ligand 21 (CCL21) in normal conjunctivae and excised pterygia collected in surgery were analyzed by qRT-PCR and immunofluorescence staining. The phenotype of CCR7-expressing cells was identified by double-staining with major histocompatibility complex II (MHC II), CD11b and CD11c.Results: The level of CCR7 was significantly increased with 9.6-fold in pterygium when compared with normal conjunctivae (p=0.008). The higher expression of CCR7, the more blood vessels in pterygium ((R2=0.44, p<0.001) and more general ocular redness ((R2=0.25, p<0.001) pterygium patients had. CCR7 positive cells were co-located with D11b+, CD11c+ or MHC II in mature DCs. Immunofluorescence staining showed CCL21 co-staining with CCR7, which implied CCL21 may be a key ligand of CCR7 in pterygia, rather than CCL19. And CCR7 was significantly associated with the extension of pterygia (R2=0.083, p=0.047, β=0.192).Conclusions: Our research revealed a possible involvement of CCR7 in the migration of mature DCs and influence the progression of primary pterygia. Targeting CCR7 may be considered as a potential therapeutic approach for the treatment of pterygium.
Changes in Soil Chemical Properties Under Different Land-Use Systems and Soil Depth Layers: A Case Study from the Central Highlands of Ethiopia
Authors: Bato Yadesa , Bekele Tamrat , Demissew Sebsebe
Published: September 2020

Background: Soil chemical properties have changed under different land-use systems and soil depth layers either by increasing or decreasing. Hence, scientifically information on the soil chemical properties dynamics under different land-use systems and soil depths are crucial for best land management practices, and to avoiding ecological negative impacts of it for sustainable development. The study aimed to evaluate the soil chemical properties dynamics under different land-use systems and soil depths in the central highlands of Ethiopia. The land-use systems included natural forest, four exotic tree plantation species (Eucalyptus globules, Cupressus lusitanica, Grevillea robusta, and Pinus patula), grassland, grazing land, and cropland. Results: The analysis of variance (ANOVA) for the majority of soil chemical properties of OC, TN, Avial. P, soil pH, EC, CEC, and exchangeable bases (Ca, Mg, K, Na) were showed that significant variations among land-use systems (P<0.0001). The highest mean values of OC (3.49 % DM ), TN ( 0.31 % DM) , Avail.P (31.52 mg/kg of soil ), CEC ( 33.63 meq/100gm soil), Exch. Ca (17.13 cmol(+)/kg soil), Exch. Mg (5.37 cmol(+)/kg soil), and Exch. K ( 3.60 cmol(+)/kg soil) were observed under natural forest than others of land-use systems. The results also showed that the lowest mean values of OC (1.47 % DM), TN (0.13 %DM), soil pH (5.38), CEC (18.98 meq/100gm soil), Exch. Ca (9.93 cmol(+)/kg soil), Exch. K (1.20 cmol(+)/kg soil), and Exch. Na (0.22 cmol(+)/kg soil) were recorded under cropland than other land-use systems. The highest mean values of EC (3.47ds/m), and Exch. Na (0.60 cmol(+)/kg soil) were observed under Eucalyptus globulus plantation forest. The overall mean values of OC, TN, Avail.P, CEC, Exch. Mg, Exch. Ca, Exch. K, and Exch. Na accumulation at the topsoil layer was higher than that of the subsoil layer except for soil pH and EC. Conclusion: In general, the majority of soil chemical properties under cropland and Eucalyptus globulus plantation forest were poorer than the soils subjected to other land-use systems which indicated that changes in land use systems were significantly affected soil chemical properties.
WITHDRAWN: Discovery of mcr-1 Harboring Incl2 Plasmids from Clinical Isolates of Multiclonal E. Coli prevalent in Pakistan.
Authors: Bilal Hazrat , Rehman Tayyab ur , Khan Muhammad Asif , Hameed Fareeha , Jian Zhang gao , Han Jianxiong , Yang Xingyuan
Published: September 2020

Background: Colistin is the last resort antibiotic against multiple drug-resistant (MDR) bacteria found in clinical infections; however, the emergence of plasmid-mediated mcr-1 gene annulled the efficacy of Colistin. This study was planned to determine the prevalence of mcr genes in clinical isolates collected in Pakistan. The molecular types and plasmids of mcr-1 bearing isolates were analysed. Methods: A total of 545 E. coli isolates collected from two major cities of Pakistan were screened for colistin-resistance and mcr genes from June 2018 to September 2019. All positive strains were subjected to antimicrobial susceptibility testing, ESBL, and MBL detection via DDST and CDT. ESBL genes detection, molecular typing, conjugation experiment, plasmid replicon typing, S1 PFGE, and southern hybridization were performed. Results: Four (0.73%) strains of mcr-1 positive isolates were susceptible to meropenem, fosfomycin, and chloramphenicol, including one that showed moderate level resistance to chloramphenicol and fosfomycin. All four strains were ESBL positive and harbored the blaCTX-M-15 gene, while three of the isolates also harbored the blaTEM-1 gene. Molecular typing revealed that four isolates belonged to diverse clonal types, mostly (75%) from avian pathogenic E. coli lineage. The mcr-1 gene was present on ~ 60 kb Incl2 plasmid, which was successfully transconjugated. Conclusion: The mcr-1 gene's detection in diverse clonal types from MDR clinical isolates enforces priority basis large scale surveillance studies followed by corrective actions to prevent the spread of the mcr-1 gene in clinical, poultry, and environmental setting.
Near-complete Loss of Fire-resistant Primary Tropical Forest Cover in Sumatra and Kalimantan
Authors: Nikonovas Tadas , Spessa Allan , Doerr Stefan , Clay Gareth , Mezbahuddin Symon
Published: September 2020

Deforestation in Sumatra and Kalimantan in recent decades has made increasingly large parts of the region vulnerable to fires. Burning is particularly widespread in deforested peatlands, leading to globally significant carbon emissions. Here we assess primary forest cover loss and fragmentation combined with active fire observations between 2001 and 2019 for the entire region. We find that fires did not penetrate undisturbed primary forest areas deeper than two kilometres from the forest edge irrespective of drought conditions, highlighting the resistance of such forests to burning. However, only 10% of primary forest on peatland and 13% on non-peatlands remain in this category, with the rest being severely fragmented or degraded due to proximity to the forest edge. Fire-resistant forests now cover only 3% of peatlands and 4.5% of non-peatlands in Sumatra and Kalimantan. We also show that 15% and 17% of burning during the 2015 and 2019 fire episodes respectively occurred on land cleared since 2001. Our work demonstrates that protection and regeneration of the remaining contiguous primary forest blocks, as well as peatland restoration, are an urgent necessity for mitigating the impacts of potentially more frequent fire events under future global warming in Indonesia.
Unpacking The Autism Spectrum Disorder Profile of Children With De Novo Disruptive GRIN2B Variants: A Quantitative and Qualitative Analysis
Authors: Hudac Caitlin M. , Kurtz-Nelson Evangeline , Rutter Tara M. , Peterson Jessica L. , Hoekzema Kendra , Beighley Jennifer , Kelly Morgan E. , Wallace Arianne S. , Gerdts Jennifer , Eichler Evan E. , Bernier Raphael A. , Earl Rachel K.
Published: September 2020

Background: Disruptive mutations to GRIN2B are considered a high-confidence genetic factor of autism spectrum disorder (ASD), yet only ~25% of patients with GRIN2B mutations are given a formal ASD diagnosis. Here, we generate a comprehensive understanding of the ASD profile in children with de novo disruptive GRIN2B variants, including social communication deficits and restricted and repetitive behaviors (RRBs).Methods: The ASD profile was measured in 34 children with disruptive GRIN2B variants, including clinically assessed ASD severity (subset of 16 children) and parental reports of ASD symptomology. GRIN2B ASD profiles were compared to 96 children with a different pathogenic ASD-associated mutation, as well as 55 children with ASD stratified based upon low (n = 14) or average (n = 41) nonverbal cognition. Following statistical group comparisons, unique GRIN2B ASD features were further characterized using qualitative analysis of clinical observations and case reports. Results: Both GRIN2B and pathogenic groups exhibited similar proportions of formal ASD diagnoses and clinically assessed ASD severity was similar across comparison groups. Parents endorsed fewer problematic social behaviors and impairing RRBs in certain categories, highlighting relative strengths in socioemotional reciprocity and fewer reported problems with rigidity or restricted interests. Contextualization of behaviors based upon clinical observations indicated a unique GRIN2B profile describing high motivation to approach others, but often inappropriate social responses. Atypical nonverbal communication and sensory seeking were evident. Limitations: This study is limited by the small sample size for the clinically assessed GRIN2B subset. Second, the ASD comparison group was older than the GRIN2B or Pathogenic groups. Lastly, we focus on ASD symptomology and do not fully characterize other psychiatric, behavioral, or medical comorbidities or link the ASD profile to the specific function of GRIN2B variants (e.g., loss or gain of function), which may be a critical next step for developing targeted interventions.Conclusions: We identify a unique ASD profile for children with disruptive GRIN2B variants involving strengths in social motivation/approach and reduced impairing RRBs. Continued work phenotyping GRIN2B should incorporate nuanced examination of these strengths in the context of the challenges faced by individuals with disruptive GRIN2B variants.
Necrotizing Funisitis As an Intrauterine Manifestation of Cryopyrin-Associated Periodic Syndrome: A Case Report and Review of The Literature
Authors: Yokoi Kyoko , Minamiguchi Sachiko , Honda Yoshitaka , Kobayashi Mizuho , Kobayashi Satoru , Nishikomori Ryuta
Published: September 2020

Background: Cryopyrin-associated periodic syndrome (CAPS) is a life-long, autoinflammatory disease associated with a gain-of-function mutation in the nucleotide-binding domain, leucine-rich repeat family, pyrin domain containing 3 (NLRP3) gene, which result in uncontrolled production of IL-1β and chronic inflammation. Chronic infantile neurologic cutaneous and articular (CINCA) syndrome/neonatal-Onset multisystem inflammatory disease (NOMID) is the most severe form of CAPS. Although the first symptoms may be presented at birth, there are few reports on the involvement of the placenta and umbilical cord in the disease. Therefore, we present herein a preterm case of CINCA/NOMID syndrome and confirms intrauterine-onset inflammation with conclusive evidence by using fetal and placental histopathological examination. Case presentation: The female patient was born at 33weeks of gestation by emergency caesarean section and weighted at 1,514 g. The most common manifestations of CINCA/NOMID syndrome including recurrent fever, urticarial rash, and ventriculomegaly due to aseptic meningitis were presented. She also exhibited atypical symptoms such as severe hepatosplenomegaly with cholestasis. The genetic analysis of NLRP3 revealed a heterozygous c.1698C>A (p.Phe566Leu) mutation, and she was diagnosed with CINCA/NOMID syndrome. Further, a histopathological examination revealed necrotizing funisitis, mainly inflammation of the umbilical artery, along with focal neutrophilic and lymphocytic villitis. Conclusion: The necrotizing funisitis, which only involved the artery, was an unusual observation for chorioamnionitis. These evidences suggest that foetal inflammation, probably due to overproduction of IL-1β, caused tissue damage in utero, and the first symptom of a newborn with CINCA/NOMID.
Vibration Preconditioning as a Method to Reduce Hyperemia Responses to Plantar Pressure-Induced Ischemia
Authors: Liu Hongmei , Ren Weiyan , Duan Yijie , Ye Wenqiang , Jan Yih-Kuen , Pu Fang , Fan Yubo
Published: September 2020

Background: One of the main causes for foot ulcers in diabetics is an excessive, constant load on the foot which over time leads to ischemia. The aim of this study is to explore whether vibration preconditioning can alleviate pressure-induced hyperemia responses in foot tissue.Methods: Fifteen healthy subjects were subjected to compression stimulation with or without vibration preconditioning, determined randomly. Skin blood flow and temperature were measured under the first metatarsal head of the right foot for each subject and compared across the test group.Results: The results showed that both test groups displayed a reactive hyperemia response, but the peak hyperemia was significantly decreased when vibration was used in combination with compression. In the group subjected to compression only (no vibration), the plantar skin temperature during the first minute after compression was significantly higher than the basal temperature, but this was not so when vibration was applied.Conclusions: The results of this study suggest that vibration preconditioning before the application of compression can decrease the degree of reactive hyperemia and alleviate pressure-induced ischemic damage. These findings may be used to develop methods to protect against pressure-induced foot lesions in diabetic people.
A Treatment Combined Prussian Blue Nanoparticles With Low-intensity Pulsed Ultrasound Alleviates Cartilage Damage in Knee Osteoarthritis by Initiating PI3K/Akt/mTOR Pathway
Authors: Zuo Deyu , Tan Botao , Jia Gongwei , Wu Dandong , Yu Lehua , Jia Lang
Published: September 2020

Background: Reactive oxidative stress (ROS) related apoptosis in chondrocytes and extracellular matrix (ECM) degradation play crucial roles in the process of osteoarthritis (OA). Prussian blue nanoparticles (PBNPs) are known to scavenge ROS in cellular. Low-intensity pulsed ultrasound (LIPUS) has been used as a non-invasive modality for the is widely used in clinical rehabilitation management of OA. Methods: In this study, we aim to investigate the effects of PBNPs/LIPUS combined treatment on knee osteoarthritis (KOA) and to determine whether phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway mediates this process. Use LPS to process primary cells of knee joint cartilage to establish a cartilage knee arthritis model. After treated with LIPUS and PBNPs, cell viability was rated by CCK-8 and ROS levels were assessed by DCFH-DA. Cell apoptosis was estimated by flow cytometry and TUNEL staining. Articular pathological changes were observed by naked eyes, H&E, and Safranin O staining, then monitored by cartilage lesion grades and Mankin’s score. Protein levels of cleaved caspase-3, Bcl-2, Bax, p-PI3K, PI3K, p-Akt, Akt, p-mTOR, mTOR, IL-1β, MMP3, MMP13, p-JINK, JINK, p-c-Jun, and c-Jun were subjected to western blot. Results: Cellular ROS, apoptosis rate, and TUNEL staining of chondrocytes were fairly decreased in the PBNPs group and the LIPUS group but drastically down-regulated in the PBNPs/LIPUS combination treatment group when compared with the LPS group. Both PBNPs and LIPUS decreased the grades of cartilage lesions and Mankin scores of knee articular cartilage, while combined administration achieved more reduction. Western blot results showed that the cleaved caspase-3, Bax, IL-1β, MMP3 and MMP13 in the PBNPs and LIPUS groups slightly decreased, and Bcl2 increased slightly, while in the combination treatment group, the former was significantly decreased, and Bcl2 was Significantly increased. Conclusion: The PBNPs/LIPUS combination treatment increased protein levels of p-PI3K, p-Akt, and p-mTOR but decreased the protein levels of p-JINK and p-c-Jun, in vitro and vivo. The PBNPs/LIPUS combination treatment reduced cellular ROS, apoptosis, and matrix metalloproteinases (MMPs), as a consequence, alleviated articular cartilage damage in KOA. Moreover, the PBNPs/LIPUS combination treatment suppressed the JINK/c-Jun signal pathway.
Prostate Cancer-Secreted Exosomal miR-1275 Modulates Osteoblast Proliferation and Differentiation by Targeting SIRT2/RUNX2 Cascade
Authors: Zou Zihao , Dai Ranran , Deng Nan , Su Wei , Liu Ping
Published: September 2020

BackgroundCancer-derived exosomes can promote tumor metastasis by delivering miRNAs from cancer cells to recipient cells. miR-1275 is suggested to be an important regulator involved in prostate cancer (PCa) metastasis; however, the association of miR-1275 with the microenvironment of PCa bone metastasis is unclear.Materials and methodsThe level of relative genes and proteins were detected by qPCR and western-blot, cell ability were examined by CCK-8 , the identification of target gene was analyzed by dual-luciferase reporter assay,exosomes were isolated from the PC3-derived conditioned medium by ultracentrifugation.Resultwe found that miR-1275 could be transferred from PCa cells to osteoblasts via exosomes. Exosomal miR-1275 significantly accelerated the proliferation of osteoblasts and the expression levels of osteoblast-specific genes, such as osteocalcin (OCN), type I collagen (COL-1), and osteopontin (OPN). Moreover, exosomal miR-1275 increased the expression of RUNX2, a master modulator of osteoblast differentiation, by down-regulation of SIRT2, which in turn influenced the proliferation and differentiation of osteoblasts.ConclusionsOur findings indicate that exosomal miR-1275 is a promoter of osteoblast activity, which may contribute to PCa bone metastasis.
Respiratory Rehabilitation After Blood Transfusion in a COVID-19 Patient: A Case Report
Authors: Mousavi Mohammad Javad , Obeidi Narges , keshmiri Saeed , Azodi Farzan , Kiyani Jamile , Abbasi Farhad
Published: September 2020

Background: The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified as the most crucial threat of the century. Due to severe pneumonia and acute respiratory distress syndrome (ARDS), the SARS-CoV-2 can cause shortness of breath, hypoxemia, and the need to mechanical ventilation, intensive care unit (ICU) management, and eventual death. We have tried to use a non-invasive approach to prevent patient from needing respiratory support with invasive ventilation (IV). Here, for the first time, improvement of oxygen delivery and oxygen saturation levels were observed in a COVID-19 patient using packed red blood cells (PRBCs) transfusion.Case presentation: A 63-year-old man with a history of smoking and addiction who came to our hospital facility with fever, shortness of breath and decreased blood oxygen saturation. High-resolution chest CT revealed bilateral and multifocal ground-glass opacities consistent with COVID-19. Subsequently, the COVID-19 infection was confirmed by real-time polymerase chain reaction (qRT-PCR) assay of the upper respiratory tract. Conclusions: Oxygen delivery and oxygen saturation improvement were observed in the COVID-19 patient, after PRBCs transfusions.
TÜRKİYE'DE HANEHALKI SORUMLUSUNUN TOPLAM GELİRİNE ETKİ EDEN BELİRLEYİCİLERİN KANTİL REGRESYON YÖNTEMİ İLE ANALİZİ
Authors: ÇOBANOĞLU Ferit , YILMAZ Halil İbrahim
Published: September 2020

Bu çalışmada, Türkiye’de hanehalkı sorumlusunun
elde etmiş olduğu yıllık toplam gelirindeki farklılık, kantil regresyon yöntemi
ile analiz edilmiştir. Araştırmada, Türkiye İstatistik Kurumu (TÜİK) Başkanlığı'ndan
sağlanan “Hanehalkı Bütçe Araştırması 2015 yılı”  mikro veri setinden yararlanılmıştır.
Çalışmada, hanehalkı sorumlusunun yıllık toplam geliri (TYG) bağımlı değişken
olarak alınırken, bağımsız değişkenler olarak ise hanehalkı sorumlusu için; cinsiyet
(C), yaş (Y) sağlık sigortasına sahip olma durumu (SS), günlük faaliyete engel
fiziksel ya da zihinsel problem olup-olmadığı (GFFZP), çalışmaya engel fiziksel
ya da zihinsel problem olup-olmadığı (CFZP), eğitim durumu (ED), medeni durum
(MD) ve çalışma durumu (CD) alınmıştır.  Koşullu
medyan regresyonu analizine göre, hanehalkı sorumlusunun elde etmiş olduğu
yıllık toplam gelirinin medyanı 18880 TL olarak belirlenmiştir. Hanehalkı
sorumlusunun elde etmiş olduğu yıllık toplam gelirinin 25. yüzdebirlik değeri
12360 TL olarak belirlenirken, 75. yüzdebirlik değeri 30000 TL olarak
belirlenmiştir. Hanehalkı sorumlusunun elde etmiş olduğu yıllık toplam
gelirinin 5. yüzdebirlik değeri 3810 TL olarak hesaplanmış iken, 95.
yüzdebirlik değeri 60000 TL olarak belirlenmiştir. Sabit (robust) kantil
regresyon analizinde, bağımsız değişkenlerin hepsinin, TYG değişkeni üzerindeki
etkisinin istatiksel olarak önemli olduğu belirlenmiştir.
Subsistencia, dieta, salud y enfermedad en el pasado: del paradigma osteológico a la era molecular en Colombia
Authors: Corcione María Antonieta , Rodríguez Freddy
Published: September 2020

En las últimas décadas, la bioarqueología colombiana ha tenido un desarrollo prominente tanto en la aplicación de nuevas técnicas como en la maduración de las conceptualizaciones teóricas en líneas temáticas sobre la subsistencia, la dieta y la salud, y enfermedad de las poblaciones prehispánicas que habitaron el territorio nacional. A diferentes escalas de análisis, los investigadores colombianos han resuelto interrogantes desde perspectivas multidisciplinares como la ecológica, evolutiva y biocultural utilizando cada vez más, técnicas radiológicas, histológicas y moleculares para la consecución de información. Este artículo de revisión tuvo como objetivo realizar un barrido de las investigaciones generadas en torno a los debates bioarqueológicos sobre adaptación al medio, migración y colonización de nuevos nichos, dieta, marcadores de actividad y condiciones de vida, principalmente.  Sin que sea una exploración exhaustiva de la gran producción investigativa en el país, esta revisión ha permitido comprender los nuevos panoramas analíticos que se están generando entre los antropólogos de las diferentes regiones y de esta forma realizar una reflexión acerca de las nuevas sinergias que se promueven en la ciencia bioarqueológica colombiana.