bluuebunny/crossref_metadata_2025 huggingface dataset:
Excessive and Problematic Internet Use During the Coronavirus Disease 2019 School Closure: Comparison Between Japanese Youth With and Without Autism Spectrum Disorder
Authors: Kawabe Kentaro , Hosokawa Rie , Nakachi Kiwamu , Yoshino Ayumi , Horiuchi Fumie , Ueno Shu-ichi
Published: January 2021

Internet use in the youth has increased manifold during the coronavirus disease 2019 (COVID-19) pandemic. Individuals with autism spectrum disorder (ASD) generally have a higher risk of problematic internet use. The aim of this study is to investigate the differences in internet and related digital media use between children with ASD and their typically developing counterparts during the COVID-19 pandemic. In this online survey in Japan conducted from April 30 to May 8, 2020, we analyzed digital media time of 84 children with ASD and 361 age- and gender-matched controls before and after school closure. Digital media use duration was significantly longer in the ASD group than in the control group before the pandemic. The increase of media use time was more prominent in the control group than in the ASD group. We observed excessive Internet use among children with ASD and without ASD, especially during the COVID-19 pandemic. It is necessary to establish strategies to prevent excessive internet use in not only children and adolescents with ASD but also without ASD in the post-pandemic world.
Veterinarians in a Changing Global Climate: Educational Disconnect and a Path Forward
Authors: Kramer Collin G. , McCaw Katherine A. , Zarestky Jill , Duncan Colleen G.
Published: January 2021

Objective: To synthesize the beliefs, knowledge and interest of veterinarians on the relationship between veterinary medicine and climate change, with the intent to identify any educational gaps and opportunities.

Sample: Responses from 560 U.S., and 54 non-U.S. veterinarians.

Procedures: An anonymous, online survey of veterinarians was distributed through electronic media, state and professional associations, and a veterinary magazine advertisement. The survey was conducted between July 1st and December 31st of 2019.

Results: Overall, veterinary respondents were confident that climate change is happening, is caused by human activities, and is impacting both human and animal health. Veterinarians also agreed that the profession should have an advocacy role in educating the public on climate change and its health impacts, particularly in clinical practices where environmental sustainability promotion can be shared with clients. Although veterinarians agreed the profession needs to be involved with climate change advocacy, most reported having had no educational opportunities within their veterinary medicine curriculum or access to continuing education on climate change.

Conclusions and Clinical Relevance: The results highlight the need for the development of educational opportunities on the topic of climate change such that veterinarians are equipped to address their concerns about current and future animal health threats.
Prostaglandin E1 Is an Efficient Molecular Tool for Forest Leech Blood Sucking
Authors: Zheng Fenshuang , Zhang Min , Yang Xingwei , Wu Feilong , Wang Gan , Feng Xingxing , Ombati Rose , Zuo Ruiling , Yang Canju , Liu Jun , Lai Ren , Luo Xiaodong , Long Chengbo
Published: January 2021

From a survival perspective, it is hypothesized that leech saliva exhibits certain physiological effects to ensure fast blood-feeding, including analgesia, anesthesia, and anti-inflammation to stay undetected by the host and vasodilatation and anti-hemostasis to ensure a steady, rapid, and sustained blood flow to the feeding site. Many anti-hemostatic compounds have been identified in leech saliva, such as hirudin, calin, and bdellin A. However, no specific substance with direct vasodilatory and anti-inflammatory function has been reported from forest leech saliva. Herein, using activity-guided analysis, prostaglandin E1 (PGE1) was identified for the first time as an efficient molecular tool for forest leech blood sucking. The structure of PGE1 was analyzed by nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectroscopy. PGE1 was found to be primarily distributed in the leech salivary gland (1228.36 ng/g body weight). We also analyzed how forest leech PGE1 affects platelet aggregation, skin vascular permeability, bleeding time, and pain. Results indicated that PGE1 efficiently inhibited platelet aggregation induced by adenosine diphosphate (ADP) (5 μM) with an IC50of 21.81 ± 2.24 nM. At doses of 10, 100 nM, and 1 μM, PGE1 increased vascular permeability by 1.18, 5.8, and 9.2 times. It also prolonged bleeding time in a concentration-independent manner. In the formalin-induced mouse paw pain model, PGE1 suppressed acute pain. To the best of our knowledge, this is the first report on PGE1 in invertebrates. The functions of PGE1, such as vasodilation, platelet aggregation inhibition, anti-inflammation, and pain alleviation, may facilitate the ingestion of host blood by leeches.
Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm
Authors: Meena Surendra Kumar , Pandey Renu , Sharma Sandeep , Gayacharan  , Kumar Tarun , Singh Madan Pal , Dikshit Harsh Kumar
Published: January 2021

To understand the physiological basis of tolerance to combined stresses to low phosphorus (P) and drought in mungbean (Vignaradiata (L.) R. Wilczek), a diverse set of 100 accessions were evaluated in hydroponics at sufficient (250 μM) and low (3 μM) P and exposed to drought (dehydration) stress. The principal component analysis and ranking of accessions based on relative values revealed that IC280489, EC397142, IC76415, IC333090, IC507340, and IC121316 performed superior while IC119005, IC73401, IC488526, and IC325853 performed poorly in all treatments. Selected accessions were evaluated in soil under control (sufficient P, irrigated), low P (without P, irrigated), drought (sufficient P, withholding irrigation), and combined stress (low P, withholding irrigation). Under combined stress, a significant reduction in gas exchange traits (photosynthesis, stomatal conductance, transpiration, instantaneous water use efficiency) and P uptake in seed and shoot was observed under combined stress as compared to individual stresses. Among accessions, IC488526 was most sensitive while IC333090 and IC507340 exhibited tolerance to individual or combined stress. The water balance and low P adaptation traits like membrane stability index, relative water content, specific leaf weight, organic acid exudation, biomass, grain yield, and P uptake can be used as physiological markers to evaluate for agronomic performance. Accessions with considerable tolerance to low P and drought stress can be either used as ‘donors’ in Vigna breeding program or cultivated in areas with limited P and water availability or both.
Plant Growth, Yields and Fruit Quality of Processing Tomato (Solanum lycopersicon L.) as Affected by the Combination of Biodegradable Mulching and Digestate
Authors: Morra Luigi , Cozzolino Eugenio , Salluzzo Antonio , Modestia Francesco , Bilotto Maurizio , Baiano Salvatore , del Piano Luisa
Published: January 2021

In order to improve environmental sustainability of tomato cultivation and the quality of the harvested fruits, we tested (a) the digestate from anaerobic fermentation of buffalo slurries as partial replacing of NP fertilizers and (b) the biodegradable mulching to improve the nutrients and water availability for crop and to control weeds. In 2017–2018, a private farm of Campania region hosted a trial with four treatments deriving from the combination of two experimental factors: (1) fertilization strategy (standard farm NPK fertilization vs. digestate combined with reduced rates of NP fertilizers); (2) soil mulching (biodegradable mulching vs. no mulching). We measured fresh and dry aboveground biomass (fruits and stem + leaves), yields, fruits quality. Results pointed out: (1) combination of digestate with reduced rates of NP fertilizers did not decrease yields compared to complete mineral fertilization; (2) yields were improved in 2017 by synergic effects of soil mulching and combination of digestate and reduced rates of NP fertilizers; (3) in both the years, digestate combined with reduced rates of NP fertilizers and soil mulching determined the significant improving of fruits quality parameters interesting the processing industry, namely, fruit color, and firmness, total soluble solids, titratable acidity while antioxidant activity, contents of ascorbic acid, polyphenols, flavonoids, and lycopene showed responses variable with year or cultivar.
Metabolomics Analysis Reveals the Mechanism of Hydrogen Cyanamide in Promoting Flower Bud Break in Blueberry
Authors: Wang Hao , Xia Xiuying , An Lijia
Published: January 2021

Bud dormancy of deciduous fruit trees is a complex process that allows trees to survive long periods in adverse conditions during winter. Dormancy is a major obstacle for both fruit production in mild winter areas and off-season culture of fruit trees in protection facilities. It is very economically advantageous to be able to control the time point of bud break and consequently harvest in crops with high returns and short harvest seasons like blueberry (Vaccinium spp.). Hydrogen cyanamide (H2CN2 (HC)) treatment is an effective method to promote dormancy release and synchronize bud break in perennial deciduous fruit trees, including blueberry. However, there are few systematic studies of the metabolic changes that occur during HC-induced bud breaking. In this study, the metabolome of blueberry buds under forced conditions following HC and water treatment (control) was analyzed using gas chromatography paired with time-of-flight mass spectrometry (GC–TOFMS) technology. A total of 252 metabolites were identified and 16 differential metabolites (VIP > 1, p < 0.05) were detected. The levels of several soluble sugars (fructose, glucose, maltose), organic acids (citric acid, alpha-ketoglutaric, succinic acid), and amino acids (aspartic acid, glutamic acid, phenylalanine) were upregulated, while tyrosine, tryptophan, and asparagine were significantly downregulated in HC-treated buds when compared with control buds. The synthesis and accumulation of phenylpropanoids (salicin, 4-vinylphenol, neohesperidin) were also promoted by HC. These results suggest that alteration of carbohydrate and amino acid metabolism, tricarboxylic acid (TCA) cycle increase, and phenylpropanoid accumulation were crucial in HC-promoted bud breaking in blueberry. This research extends our understanding of the mechanisms involved in dormancy release induced by HC and provides a theoretical basis for applying HC to accelerate bud break.
Arabidopsis APx-R Is a Plastidial Ascorbate-Independent Peroxidase Regulated by Photomorphogenesis
Authors: Lazzarotto Fernanda , Wahni Khadija , Piovesana Maiara , Maraschin Felipe , Messens Joris , Margis-Pinheiro Marcia
Published: January 2021

Peroxidases are enzymes that catalyze the reduction of hydrogen peroxide, thus minimizing cell injury and modulating signaling pathways as response to this reactive oxygen species. Using a phylogenetic approach, we previously identified a new peroxidase family composed of a small subset of ascorbate peroxidase (APx) homologs with distinguished features, which we named ascorbate peroxidase-related (APx-R). In this study, we showed that APx-R is an ascorbate-independent heme peroxidase. Despite being annotated as a cytosolic protein in public databases, transient expression of AtAPx-R-YFP in Arabidopsis thaliana protoplasts and stable overexpression in plants showed that the protein is targeted to plastids. To characterize APx-R participation in the antioxidant metabolism, we analyzed loss-of-function mutants and AtAPx-R overexpressing lines. Molecular analysis showed that glutathione peroxidase 7 (GPx07) is specifically induced to compensate the absence of APx-R. APx-R overexpressing lines display faster germination rates, further confirming the involvement of APx-R in seed germination. The constitutive overexpression of AtAPx-R-YFP unraveled the existence of a post-translational mechanism that eliminates APx-R from most tissues, in a process coordinated with photomorphogenesis. Our results show a direct role of APx-R during germinative and post-germinative development associated with etioplasts differentiation.
Effectiveness of Psychostimulant and Non-Psychostimulant Drug Therapy in the Attention Deficit Hyperactivity Disorder
Authors: Cojocaru Adriana , Hogea Lavinia Maria , Poroch Vladimir , Simu Mihaela Adriana , Enatescu Virgil Radu , Jeleriu Roxana , Andreescu Nicoleta Ioana , Puiu Maria , Hogea Bogdan Gheorghe , Grigoras Mirela , Folescu Roxana , Zamfir Carmen Lăcrămioara , Enatescu Ileana , Nussbaum Laura Alexandra
Published: January 2021

Attention Deficit Hyperkinetic Disorder (ADHD) is a neurobiological behavioral disorder of the child, adolescent, and adult characterized by problems of concentration, hyperactivity, and impulsivity caused by an imbalance of chemical neurotransmitters in the brain—dopamine and noradrenaline. ADHD first-line drugs are divided in psychostimulant, as Methylphenidate and Amphetamines and non-psychostimulant medications-Atomoxetine (the only representative non-psychostimulant medication approved in our country in children and adolescents). The purpose of our research was to assess the clinical evolution of patients with ADHD based on the drug treatment that is administered: psychostimulant or non-psychostimulant. Both psychostimulant—Methylphenidate, and non-psychostimulant therapy—Atomoxetine, proved to significantly improve the symptoms of attention deficit hyperkinetic disorder. There was a significant reduction in the severity of ADHD symptoms at six months and at one year from the start of treatment in the case of the psychostimulant group, whereas in the non-psychostimulant group, the significant reduction in severity of symptomatology occurs only at six months after the start of treatment. We can conclude that both types of drugs are effective in reducing the severity of symptoms and in improving the clinical condition of patients with ADHD, but the comparative analysis of the two groups demonstrated that significantly better results are obtained with psychostimulant treatment.
Roll Control of Morphing Aircraft with Synthetic Jet Actuators at a High Angle of Attack
Authors: Li Wencheng , Wang Wenyun , Huang Xiaomao , Zhang Shun , Li Chenyang
Published: January 2021

Flow separation and dynamic stall occurring at a high angle of attack will lead to difficulty in control and maneuverability for morphing aircraft. This study proposes a novel active flow control technology using a synthetic jet actuator for the roll motion of morphing aircraft. With the help of the computational fluid dynamics method and vortex lattice method, the roll control model of morphing aircraft undergoing large shape change at a high angle of attack is established. In this model, both the array of the actuator with an optimized cavity shape and morphing span, which are subject to the input saturation constraint, are used to mimic the conventional control surface. Integrated flight control based on the sliding mode control method is designed to ensure the desired closed-loop asymptotic stability, wherein the radial basis function neural network is employed to provide the compensation induced by the input saturation constraint. To demonstrate the effectiveness of the control scheme, various control strategies for different combinations of input are proposed to maintain the roll motion. The numerical results show that the designed control law could track the target signal well, which suggests that the virtual control surface is an effective tool for maintaining the high flight performance of morphing aircraft.
Experimental and Numerical Studies on the Structural Performance of a Double Composite Wall
Authors: Han Sun-Jin , Heo Inwook , Kim Jae-Hyun , Kim Kang-Su , Oh Young-Hun
Published: January 2021

In this study, experiments and numerical analyses were carried out to examine the flexural and shear performance of a double composite wall (DCW) manufactured using a precast concrete (PC) method. One flexural specimen and three shear specimens were fabricated, and the effect of the bolts used for the assembly of the PC panels on the shear strength of the DCW was investigated. The failure mode, flexural and shear behavior, and composite behavior of the PC panel and cast-in-place (CIP) concrete were analyzed in detail, and the behavioral characteristics of the DCW were clearly identified by comparing the results of tests with those obtained from a non-linear flexural analysis and finite element analysis. Based on the test and analysis results, this study proposed a practical equation for reasonably estimating the shear strength of a DCW section composed of PC, CIP concrete, and bolts utilizing the current code equations.
The Effect of Laser Nitriding on Surface Characteristics and Wear Resistance of NiTi Alloy with Low Power Fiber Laser
Authors: Wang Hao , Nett Ralf , Gurevich Evgeny L. , Ostendorf Andreas
Published: January 2021

The laser nitriding was performed in nitrogen gas at room temperature (20 °C) and low temperature (−190 °C) by a low power fiber laser to modify the wear and abrasion resistance of NiTi alloy. The surface roughness and element composition were analyzed by roughness device and energy-dispersive X-ray spectroscopy respectively. The results of roughness show that laser treatment can change the surface roughness due to the laser remelting. The effect of laser nitriding on the microhardness, friction coefficient, and worn scars of NiTi alloy was also studied, which shows that the microhardness of the NiTi alloy increases after laser nitriding. The optical microscope and scanning electron microscope were used to characterize the surface of NiTi alloy after wear testing to observe the microstructure of worn scars. The results show that the laser nitriding with different parameters can induce a nitride layer with different thicknesses and the higher energy deposition is the key factor for the formation of the nitride layer, which can decrease the friction coefficient and reduce wear loss during the application of NiTi alloy. The improvement of wear resistance can be attributed to the hard nitriding layer.
Aspects of Industrial Design and Their Implications for Society. Case Studies on the Influence of Packaging Design and Placement at the Point of Sale
Authors: David Juárez-Varón , Ana Mengual-Recuerda , Santiago Ferrándiz-Bou , Faustino Alarcón-Valero
Published: January 2021

Manufacturing engineering is responsible for the design, development and improvement of production systems that convert raw materials into finished products. Each product is designed to be sold to numerous potential consumers, so the importance of the stimuli surrounding the product in packaging, and at the point of sale, cannot be underestimated. The environmental, social, and ethical commitments of industrial design (and their implications in manufacturing) are establishing universal principles in a common effort to foster a more harmonious and sustainable society. This work aims to analyse, through eye tracking biometric techniques, the level of saturation of information generated by the concentration of stimuli in packaging and the retail channel, possibly creating a lower level of attention towards the product itself. This research confirms that every product associated with a manufacturing process seeks to respond to a need, so the associated responsibility is significant. This would suggest that designers incorporate knowledge from multiple fields, including marketing strategies, design, research and development, basic knowledge related to production, integration management and communication skills. More than 50% of consumer attention is dedicated to other elements/items that accompany the product, so it is important to consider this in the design phase. The results can be used to improve efficiency in both generating product attention, and stimulus design for the purchasing process.
Effects of Ultraviolet Light Irradiation on Silk Fibroin Films Prepared under Different Conditions
Authors: Lee Sora , Kim Soo Hyun , Jo You-Young , Ju Wan-Taek , Kim Hyun-Bok , Kweon HaeYong
Published: January 2021

Silk fibroin (SF)-based materials are exposed to both natural and artificial ultraviolet (UV) light during preparation or administration. However, the effects of UV irradiation on SF films prepared under different conditions have not yet been described in detail. In this study, four SF films with different molecular weight (MW) distribution were fabricated using SF solutions, which were prepared by dissolving degummed SF for 0.5–24 h. We observed UV (365 nm) irradiation on SF films induced the increase of yellowness and absorbance at 310 nm of SF films, indicating the formation of new photo-products and di-tyrosine bonds by photo-oxidation. Due to di-tyrosine cross-links between SF chains, UV-irradiated SF films were not fully dissociated in urea solution. In addition to formation of new products, UV reduced the crystallinity of SF films by breaking hydrogen bonds of β-sheet conformation. Unlike the UV-induced decomposition of physical interactions, UV did not affect the covalent bonds (i.e., peptide bonds). Through these experiments, we could expect that SF with higher MW was more susceptible and SF with lower MW was more resistant to UV-induced photo-oxidation and photo-degradation. These results provide useful information about UV-induced aging of SF-based materials under natural sunlight and UV irradiating conditions.
ZnO Nanoparticles with Controllable Ce Content for Efficient Photocatalytic Degradation of MB Synthesized by the Polyol Method
Authors: Flores-Carrasco Gregorio , Rodríguez-Peña Micaela , Urbieta Ana , Fernández Paloma , Rabanal María Eugenia
Published: January 2021

This paper reports on the synthesis of Ce-doped ZnO (CZO) nanoparticles (NPs) by an alternative polyol method at low temperature. The method, facile and rapid, uses acetate-based precursors, ethylene glycol as solvent, and polyvinylpyrrolidone as capping agent. The effects of the Ce-doping concentration (ranging from 0 to 8.24 atomic%) on the structural, morphological, compositional, optical, luminescence, and photocatalytic properties of the NPs were investigated by several techniques. The structural findings confirmed that the CZO NPs have a typical hexagonal wurtzite-type structure with a preferred orientation along the (101) plane. The results obtained by Field Emission Scanning Electron Microscopy (FESEM) and Transmission Electron Microscopy (TEM) revealed that the NPs size decreased (from ~30 to ~16 nm) with an increase in the Ce-doping concentration. Energy Dispersive X-Ray Spectroscopy (EDS) and High Resolution Transmission Microscopy (HRTEM) results confirmed the incorporation of Ce ions into the ZnO lattice. Ce-doping influences the photoluminescence (PL) emission compared to that of pure ZnO. The PL emission is related to the presence of different kinds of defects, which could take part in charge transfer and/or trapping mechanisms, hence playing an essential role in the photocatalytic activity (PCA). In fact, in this work we report an enhancement of PCA as a consequence of Ce-doping. In this sense, the best results were obtained for samples doped with 3.24 atomic%, that exhibited a photocatalytic degradation efficiency close to 99% after 60 min ultraviolet (UV) illumination, thus confirming the viability of Ce-doping for environmental applications.
Iron Based Core-Shell Structures as Versatile Materials: Magnetic Support and Solid Catalyst
Authors: Zambrzycki Christian , Shao Runbang , Misra Archismita , Streb Carsten , Herr Ulrich , Güttel Robert
Published: January 2021

Core-shell materials are promising functional materials for fundamental research and industrial application, as their properties can be adapted for specific applications. In particular, particles featuring iron or iron oxide as core material are relevant since they combine magnetic and catalytic properties. The addition of an SiO2 shell around the core particles introduces additional design aspects, such as a pore structure and surface functionalization. Herein, we describe the synthesis and application of iron-based core-shell nanoparticles for two different fields of research that is heterogeneous catalysis and water purification. The iron-based core shell materials were characterized by transmission electron microscopy, as well as N2-physisorption, X-ray diffraction, and vibrating-sample magnetometer measurements in order to correlate their properties with the performance in the target applications. Investigations of these materials in CO2 hydrogenation and water purification show their versatility and applicability in different fields of research and application, after suitable individual functionalization of the core-shell precursor. For design and application of magnetically separable particles, the SiO2 shell is surface-functionalized with an ionic liquid in order to bind water pollutants selectively. The core requires no functionalization, as it provides suitable magnetic properties in the as-made state. For catalytic application in synthesis gas reactions, the SiO2-stabilized core nanoparticles are reductively functionalized to provide the catalytically active metallic iron sites. Therefore, Fe@SiO2 core-shell nanostructures are shown to provide platform materials for various fields of application, after a specific functionalization.
Symmetry Breaking as an Interdisciplinary Concept Unifying Cell and Developmental Biology
Authors: Goryachev Andrew B.
Published: January 2021

The concept of “symmetry breaking” has become a mainstay of modern biology, yet you will not find a definition of this concept specific to biological systems in Wikipedia [...]
Lung Ultrasound Is Often, but Not Always, Normal in Healthy Subjects: Considerations for COVID-19 Pandemic
Authors: Raiteri Alberto , Alvisi Margherita , Serio Ilaria , Stefanini Federico , Tovoli Francesco , Piscaglia Fabio
Published: January 2021

Background: Lung ultrasound (LU) is becoming an increasingly important diagnostic tool in detecting lung involvement in Corona Virus Disease 2019 (COVID-19). The aim of this study was to ascertain the likelihood of finding LU abnormalities; mimicking lung involvement; in COVID-19 negative healthy individuals. Methods: We performed LU on 265 healthcare workers; not presenting COVID-19 major symptoms and in good health; during the course of a serological screening program for COVID-19 in our General Hospital. LU results were reported as total Lung Ultrasound Score (LUS) using a 12-zone method of reporting. Results: 250/265 subjects were included in the COVID-19 negative group. LU was not completely normal (LUS ≠ 0) in 65/250 COVID-19 negative subjects (26%) and in 12/15 (80%) poorly symptomatic COVID-19 positive subjects; with a multifocal pattern in 12.7% vs. 66.7% of cases respectively. Age and COVID-19 positivity were independent predictors of total LUS. A total LUS ≥ 2 had a sensitivity of 66.67% and a specificity of 85.60% in detecting COVID-19 positivity. Conclusions: A slightly altered LU can be quite frequent in healthy COVID-19 negative subjects. LU can have a role in confirming but not screening COVID-19 poorly symptomatic cases.
MUTYH as an Emerging Predictive Biomarker in Ovarian Cancer
Authors: Hutchcraft Megan L. , Gallion Holly H. , Kolesar Jill M.
Published: January 2021

Approximately 18% of ovarian cancers have an underlying genetic predisposition and many of the genetic alterations have become intervention and therapy targets. Although mutations in MutY homolog (MUTYH) are best known for MUTYH associated polyposis and colorectal cancer, it plays a role in the development of ovarian cancer. In this review, we discuss the function of the MUTYH gene, mutation epidemiology, and its mechanism for carcinogenesis. We additionally examine its emerging role in the development of ovarian cancer and how it may be used as a predictive and targetable biomarker. MUTYH mutations may confer the risk of ovarian cancer by the failure of its well-known base excision repair mechanism or by failure to induce cell death. Biallelic germline MUTYH mutations confer a 14% risk of ovarian cancer by age 70. A monoallelic germline mutation in conjunction with a somatic MUTYH mutation may also contribute to the development of ovarian cancer. Resistance to platinum-based chemotherapeutic agents may be seen in tumors with monoallelic mutations, but platinum sensitivity in the biallelic setting. As MUTYH is intimately associated with targetable molecular partners, therapeutic options for MUTYH driven ovarian cancers include programed-death 1/programed-death ligand-1 inhibitors and poly-adenosine diphosphate ribose polymerase inhibitors. Understanding the function of MUTYH and its associated partners is critical for determining screening, risk reduction, and therapeutic approaches for MUTYH-driven ovarian cancers.
High Entropy Alloys as Filler Metals for Joining
Authors: Luo Dan , Xiao Yong , Hardwick Liam , Snell Robert , Way Matthew , Sanuy Morell Xavier , Livera Frances , Ludford Nicholas , Panwisawas Chinnapat , Dong Hongbiao , Goodall Russell
Published: January 2021

In the search for applications for alloys developed under the philosophy of the High Entropy Alloy (HEA)-type materials, the focus may be placed on applications where current alloys also use multiple components, albeit at lower levels than those found in HEAs. One such area, where alloys with complex compositions are already found, is in filler metals used for joining. In soldering (<450 °C) and brazing (>450 °C), filler metal alloys are taken above their liquidus temperature and used to form a metallic bond between two components, which remain both unmelted and largely unchanged throughout the process. These joining methods are widely used in applications from electronics to aerospace and energy, and filler metals are highly diverse, to allow compatibility with a broad range of base materials (including the capability to join ceramics to metals) and a large range of processing temperatures. Here, we review recent developments in filler metals relevant to High Entropy materials, and argue that such alloys merit further exploration to help overcome a number of current challenges that need to be solved for filler metal-based joining methods.
Micro-Raman Spectroscopy of Selected Macerals of the Huminite Group: An Example from the Szczerców Lignite Deposit (Central Poland)
Authors: Bielowicz Barbara , Morga Rafał
Published: January 2021

Lignite (ulminite reflectance Rr = 0.27%) from the Szczerców deposit (Central Poland) is dominated by huminite group macerals, containing a high proportion of attrinite and densinite. Densinite and ulminite are more abundant in small aromatic units than attrinite, which may result from their stronger gelification. The differences in Raman spectral characteristics between attrinite and ulminite are more pronounced than between attrinite and densinite. Fusinite, in comparison with the huminite group macerals, is composed of larger, more varied aromatic systems. The D4 (1190–1200 cm−1) and D5 bands (1280–1290 cm−1), most likely, correspond to different chemical structures, and their origin should be further investigated.
The Role of Intact and Disintegrated Egg Yolk Low-Density Lipoproteins during Sponge Cake Making and Their Impact on Starch and Protein Mediated Structure Setting
Authors: Pycarelle Sarah C. , Bosmans Geertrui M. , Pareyt Bram , Brijs Kristof , Delcour Jan A.
Published: January 2021

The main sponge cake ingredients are flour, sucrose, eggs and leavening agents. Exogenous lipids (e.g., monoacylglycerols) are often used to increase air–liquid interface stability in the batter. There is a consumer trend to avoid foods containing such additives. We here reasoned that egg yolk may be an alternative source of surface-active lipids and set out to study the role of egg yolk lipids during sponge cake making. This was done by relocating or removing them prior to batter preparation using ethanol treatments and examining how this affects cake (batter) properties and structure setting during baking. Most egg yolk lipids occur within spherical low-density lipoproteins (LDLs) which were disintegrated by the ethanol treatments. Results showed that egg yolk lipids impact air–liquid interface stability and less so cake structure setting. To prepare high-quality sponge cakes by multistage mixing preferably intact LDLs or, alternatively, their components are needed to incorporate sufficient air during mixing and to stabilize it after mixing. It was also shown that the batter contains intact LDLs in the continuous phase and disintegrated LDLs at air–liquid interfaces. Sponge cake contains intact LDLs in the cake matrix, disintegrated LDLs at air–crumb interfaces and disintegrated LDLs incorporated into the protein network.
Analyzing Gluten Content in Various Food Products Using Different Types of ELISA Test Kits
Authors: Yu Ja Myung , Lee Jae Hoon , Park Jong-Dae , Choi Yun-Sang , Sung Jung-Min , Jang Hae Won
Published: January 2021

Gluten is an insoluble protein produced when glutelins and prolamins, which are found in grains such as wheat, barley, and oats, combine to form an elastic thin film. This dietary gluten can cause severe contraction of the intestinal mucous membrane in some people, preventing nutrient absorption. This condition, called celiac disease (CD), affects approximately 1% of the world’s population. The only current treatment for patients with CD and similar diseases is lifelong avoidance of gluten. To analyze the gluten content in food, various enzyme-linked immunosorbent assay (ELISA) tests are currently used. In this study, the gluten content in various food products was analyzed using different kinds of ELISA test kits. For gluten-free food, three different ELISA test kits mostly yielded values below the limit of detection. However, gluten was detected at 24.0–40.2 g/kg in bread, 6.5–72.6 g/kg in noodles, and 23.0–86.9 g/kg in different powder food samples. A significant difference (p < 0.05) in gluten content was observed for these gluten-containing food products. Reproducibility issues suggest that it is necessary to use several ELISA kits for the accurate detection and quantification of gluten in various food products rather than using one ELISA kit.
A Combined Metabolomic and Metagenomic Approach to Discriminate Raw Milk for the Production of Hard Cheese
Authors: Bellassi Paolo , Rocchetti Gabriele , Nocetti Marco , Lucini Luigi , Masoero Francesco , Morelli Lorenzo
Published: January 2021

The chemical composition of milk can be significantly affected by different factors across the dairy supply chain, including primary production practices. Among the latter, the feeding system could drive the nutritional value and technological properties of milk and dairy products. Therefore, in this work, a combined foodomics approach based on both untargeted metabolomics and metagenomics was used to shed light onto the impact of feeding systems (i.e., hay vs. a mixed ration based on hay and fresh forage) on the chemical profile of raw milk for the production of hard cheese. In particular, ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF) was used to investigate the chemical profile of raw milk (n = 46) collected from dairy herds located in the Po River Valley (Italy) and considering different feeding systems. Overall, a total of 3320 molecular features were putatively annotated across samples, corresponding to 734 unique compound structures, with significant differences (p < 0.05) between the two feeding regimens under investigation. Additionally, supervised multivariate statistics following metabolomics-based analysis allowed us to clearly discriminate raw milk samples according to the feeding systems, also extrapolating the most discriminant metabolites. Interestingly, 10 compounds were able to strongly explain the differences as imposed by the addition of forage in the cows’ diet, being mainly glycerophospholipids (i.e., lysophosphatidylethanolamines, lysophosphatidylcholines, and phosphatidylcholines), followed by 5-(3′,4′-Dihydroxyphenyl)-gamma-valerolactone-4′-O-glucuronide, 5a-androstan-3a,17b-diol disulfuric acid, and N-stearoyl glycine. The markers identified included both feed-derived (such as phenolic metabolites) and animal-derived compounds (such as lipids and derivatives). Finally, although characterized by a lower prediction ability, the metagenomic profile was found to be significantly correlated to some milk metabolites, with Staphylococcaceae, Pseudomonadaceae, and Dermabacteraceae establishing a higher number of significant correlations with the discriminant metabolites. Therefore, taken together, our preliminary results provide a comprehensive foodomic picture of raw milk samples from different feeding regimens, thus supporting further ad hoc studies investigating the metabolomic and metagenomic changes of milk in all processing conditions.
Hydrogen and Oxygen Production via Water Splitting in a Solar-Powered Membrane Reactor—A Conceptual Study
Authors: Tosti Silvano , Pozio Alfonso , Farina Luca , Santucci Alessia
Published: January 2021

Among the processes for producing hydrogen and oxygen from water via the use of solar energy, water splitting has the advantage of being carried out in onestep. According to thermodynamics, this process exhibits conversions of practical interest at very high temperatures and needs efficient separation systems in order to separate the reaction products, hydrogen and oxygen. In this conceptual work, the behavior of a membrane reactor that uses two membranes perm-selective to hydrogen and oxygen is investigated in the temperature range 2000–2500 °C of interest for coupling this device with solar receivers. The effect of the reaction pressure has been evaluated at 0.5 and 1 bar while the permeate pressure has been fixed at 100 Pa. As a first result, the use of the membrane perm-selective to oxygen in addition to the hydrogen one has improved significantly the reaction conversion that, for instance, at 0.5 bar and 2000 °C, moves from 9.8% up to 18.8%. Based on these critical data, a preliminary design of a membrane reactor consisting of a Ta tubular membrane separating the hydrogen and a hafnia camera separating the oxygen is presented: optimaloperating temperature of the reactor results in being around 2500 °C, a value making impracticable its coupling with solar receivers even in view of an optimistic development of this technology. The study has verified that at 2000 °C with a water feed flow rate of 1000 kg h−1 about 200 and 100 m3 h−1 of hydrogen and oxygen are produced. In this case, a surface of the hafnia membrane of the order of hundreds m2 is required: the design of such a membrane device may be feasible when considering special reactor configurations.
Feeling the Void: Lack of Support for Isolation and Sleep Difficulties in Pregnant Women during the COVID-19 Pandemic Revealed by Twitter Data Analysis
Authors: Talbot Joey , Charron Valérie , Konkle Anne TM
Published: January 2021

Pregnant women face many physical and psychological changes during their pregnancy. It is known that stress, caused by many factors and life events such as the COVID-19 pandemic, can negatively impact the health of mothers and offspring. It is the first time social media, such as Twitter, are available and commonly used during a global pandemic; this allows access to a rich set of data. The objective of this study was to characterize the content of an international sample of tweets related to pregnancy and mental health during the first wave of COVID-19, from March to June 2020. Tweets were collected using GetOldTweets3. Sentiment analysis was performed using the VADER sentiment analysis tool, and a thematic analysis was performed. In total, 192 tweets were analyzed: 51 were from individuals, 37 from companies, 56 from non-profit organizations, and 48 from health professionals/researchers. Findings showed discrepancies between individual and non-individual tweets. Women expressed anxiety, depressive symptoms, sleeping problems, and distress related to isolation. Alarmingly, there was a discrepancy between distress expressed by women with isolation and sleep difficulties compared to support offered by non-individuals. Concrete efforts should be made to acknowledge these issues on Twitter while maintaining the current support offered.
Rise of ‘Lonely’ Consumers in the Post-COVID-19 Era: A Synthesised Review on Psychological, Commercial and Social Implications
Authors: Wang Xueqin , Wong Yiik Diew , Yuen Kum Fai
Published: January 2021

Loneliness is a pervasive problem recognised as a serious social issue, and the prevailing COVID-19 pandemic has exacerbated loneliness to greater prominence and concern. We expect a rise of a massive group of ‘lonely’ consumers who are deeply entrenched in the social isolation caused by COVID-19. There is an urgent need to revisit the phenomenon of lonely consumers to better prepare academic researchers, public policy makers and commercial managers in the post-COVID-19 era. Thus, this study conducts a synthesised review on past studies of lonely consumers. Based on an inductive analysis of 56 articles, 74 key themes are identified. These key themes are further categorised into five major clusters by way of a co-occurrence network analysis. Respectively, the five clusters address the psychological implications related to the dynamics between nonhuman attachment and consumers’ loneliness, the commercial implications related to the paradoxical motivations of affiliation and self-affirmation in product selection and the dual information processing mechanism in response to advertisement appeals, and the social implications related to consumers’ well-being in an ageing society and the anthropomorphic companionship in a virtual world. A list of research questions is proposed that concludes the review study.
Predictors of Self-Reported Hand Hygiene Performance among Nurses at Tertiary Care Hospitals in East Coast Malaysia
Authors: Rahim Mohamad Hazni Abd , Ibrahim Mohd Ismail , Noor Siti Suraiya Md , Fadzil Norhana Mohamed
Published: January 2021

Background: Hand hygiene (HH) is the simplest and most effective way to reduce the incidence of healthcare-associated infections (HCAIs). Methods: This cross-sectional study aimed to determine factors associated with self-reported HH performance among nurses at Kelantan tertiary care hospitals. A sample of 438 registered nurses was selected through a stratified random sampling method. Self-reported HH performance was assessed using a validated WHO self-administered HH knowledge and perception questionnaire for healthcare workers. Results: A multiple linear regression analysis was performed to identify the predictors. The factors that significantly predicted self-reported HH performance among nurses included perception score (beta coefficient (β) = 0.260; 95% CI: 0.200, 0.417; p < 0.001), pediatric department (β = −0.104; 95% CI: −9.335, −2.467; p < 0.001), and orthopedic department (β = −5.957; 95% CI: −9.539, −0.720; p < 0.023), adjusted R2 = 0.102; p < 0.001. Nurses with a strong perception and belief in HH were more likely to have better HH performance. Compared to pediatric and orthopedic, surgical departments were associated with better self-reported HH performance. Conclusions: This study showed the importance of factors that could improve the intervention’s performance in HH strategy. Lack of perception and HH program intervention in departments engaged in patient care could lead to poor HH practices, thus increasing HCAIs and antimicrobial resistance (AMR).
Exploring Precursors of Construction Accidents in China: A Grounded Theory Approach
Authors: Li Zhen , Mao Rui , Meng Qing Feng , Hu Xin , Li Hong Xian
Published: January 2021

The implementation of precursor management can improve safety performance of construction projects through effectively managing the correlations between construction accidents and their precursors. However, a system of comprehensive knowledge about what precursors mean within the context of construction safety is still lacking. This study aims to capture the nature of precursors in the construction industry and explore the process of a precursor event evolving into a construction accident to fill this gap. Based on 135 construction accident reports in China, this study adopts grounded theory to identify different types of accident precursors and explore their interactions with the development of the accident. An indicator system of precursors for construction accidents was developed, which included two major categories of precursors: behavioral factors and physical factors and five minor categories of precursors: individual behavior factors, organizational driving factors, objective physical factors, construction environmental factors, mechanical equipment factors. In addition, a precursor management strategy that includes the three stages of identification, response and effectiveness testing was established. The results of the study reveal the correlations between precursors and construction accidents, which can promote construction professionals’ better understanding about precursors and improve their capabilities of managing precursors in practice.
Key Properties of a Bioactive Ag-SiO2/TiO2 Coating on NiTi Shape Memory Alloy as Necessary at the Development of a New Class of Biomedical Materials
Authors: Dulski Mateusz , Gawecki Robert , Sułowicz Sławomir , Cichomski Michal , Kazek-Kęsik Alicja , Wala Marta , Leśniak-Ziółkowska Katarzyna , Simka Wojciech , Mrozek-Wilczkiewicz Anna , Gawęda Magdalena , Sitarz Maciej , Dudek Karolina
Published: January 2021

Recent years have seen the dynamic development of methods for functionalizing the surface of implants using biomaterials that can mimic the physical and mechanical nature of native tissue, prevent the formation of bacterial biofilm, promote osteoconduction, and have the ability to sustain cell proliferation. One of the concepts for achieving this goal, which is presented in this work, is to functionalize the surface of NiTi shape memory alloy by an atypical glass-like nanocomposite that consists of SiO2-TiO2 with silver nanoparticles. However, determining the potential medical uses of bio(nano)coating prepared in this way requires an analysis of its surface roughness, tribology, or wettability, especially in the context of the commonly used reference coat-forming hydroxyapatite (HAp). According to our results, the surface roughness ranged between (112 ± 3) nm (Ag-SiO2)—(141 ± 5) nm (HAp), the water contact angle was in the range (74.8 ± 1.6)° (Ag-SiO2)—(70.6 ± 1.2)° (HAp), while the surface free energy was in the range of 45.4 mJ/m2 (Ag-SiO2)—46.8 mJ/m2 (HAp). The adhesive force and friction coefficient were determined to be 1.04 (Ag-SiO2)—1.14 (HAp) and 0.247 ± 0.012 (Ag-SiO2) and 0.397 ± 0.034 (HAp), respectively. The chemical data showed that the release of the metal, mainly Ni from the covered NiTi substrate or Ag from Ag-SiO2 coating had a negligible effect. It was revealed that the NiTi alloy that was coated with Ag-SiO2 did not favor the formation of E. coli or S. aureus biofilm compared to the HAp-coated alloy. Moreover, both approaches to surface functionalization indicated good viability of the normal human dermal fibroblast and osteoblast cells and confirmed the high osteoconductive features of the biomaterial. The similarities of both types of coat-forming materials indicate an excellent potential of the silver-silica composite as a new material for the functionalization of the surface of a biomaterial and the development of a new type of functionalized implants.