bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological and Electrophysiological Properties of Principal Neurons in the Rat Lateral Amygdala In Vitro
Authors: Faber E.S.L. , Callister R. J. , Sah P.
Published: December 2017

In this study, we characterize the electrophysiological and morphological properties of spiny principal neurons in the rat lateral amygdala using whole cell recordings in acute brain slices. These neurons exhibited a range of firing properties in response to prolonged current injection. Responses varied from cells that showed full spike frequency adaptation, spiking three to five times, to those that showed no adaptation. The differences in firing patterns were largely explained by the amplitude of the afterhyperpolarization (AHP) that followed spike trains. Cells that showed full spike frequency adaptation had large amplitude slow AHPs, whereas cells that discharged tonically had slow AHPs of much smaller amplitude. During spike trains, all cells showed a similar broadening of their action potentials. Biocytin-filled neurons showed a range of pyramidal-like morphologies, differed in dendritic complexity, had spiny dendrites, and differed in the degree to which they clearly exhibited apical versus basal dendrites. Quantitative analysis revealed no association between cell morphology and firing properties. We conclude that the discharge properties of neurons in the lateral nucleus, in response to somatic current injections, are determined by the differential distribution of ionic conductances rather than through mechanisms that rely on cell morphology.
Plasticity of Bat's Central Auditory System Evoked by Focal Electric Stimulation of Auditory and/or Somatosensory Cortices
Authors: Ma Xiaofeng , Suga Nobuo
Published: December 2017

Recent findings indicate that the corticofugal system would play an important role in cortical plasticity as well as collicular plasticity. To understand the role of the corticofugal system in plasticity, therefore, we studied the amount and the time course of plasticity in the inferior colliculus (IC) and auditory cortex (AC) evoked by focal electrical stimulation of the AC and also the effect of electrical stimulation of the somatosensory cortex on the plasticity evoked by the stimulation of the AC. In adult big brown bats ( Eptesicus fuscus), we made the following major findings. 1) Electric stimulation of the AC evokes best frequency (BF) shifts, i.e., shifts in frequency-response curves of collicular and cortical neurons. These BF shifts start to occur within 2 min, reach a maximum (or plateau) at 30 min, and then recover ∼180 min after a 30-min-long stimulus session. When the stimulus session is lengthened from 30 to 90 min, the plateau lasts ∼60 min, but BF shifts recover ∼180 min after the session. 2) The electric stimulation of the somatosensory cortex delivered immediately after that of the AC, as in fear conditioning, evokes a dramatic lengthening of the recovery period of the cortical BF shifts but not that of the collicular BF shift. The electric stimulation of the somatosensory cortex delivered before that of the AC, as in backward conditioning, has no effect on the collicular and cortical BF shifts. 3) Electric stimulation of the AC evokes BF shifts not only in the ipsilateral IC and AC but also in the contralateral IC and AC. BF shifts are smaller in amount and shorter in recovery time for contralateral collicular and cortical neurons than for ipsilateral ones. Our findings support the hypothesis that the AC and the corticofugal system have an intrinsic mechanism for reorganization of the IC and AC, that the reorganization is highly specific to a value of an acoustic parameter (frequency), and that the reorganization is augmented by excitation of nonauditory sensory cortex that makes the acoustic stimulus behaviorally relevant to the animal through associative learning.
Burst and Tonic Response Modes in Thalamic Neurons During Sleep and Wakefulness
Authors: Weyand Theodore G. , Boudreaux Michael , Guido William
Published: December 2017

Thalamic neurons can exhibit two distinct firing modes: tonic and burst. In the lateral geniculate nucleus (LGN), the tonic mode appears as a relatively faithful relay of visual information from retina to cortex. The function of the burst mode is less understood. Its prevalence during slow-wave sleep (SWS) and linkage to synchronous cortical electroencephalogram (EEG) suggest that it has an important role during this form of sleep. Although not nearly as common, bursting can also occur during wakefulness. The goal of this study was to identify conditions that affect burst probability, and to compare burst incidence during sleeping and waking. LGN neurons are extraordinarily heterogenous in the degree to which they burst, during both sleeping and waking. Some LGN neurons never burst under any conditions during wakefulness, and several never burst during slow-wave sleep. During wakefulness, <1% of action potentials were associated with bursting, whereas during sleep this fraction jumps to 18%. Although bursting was most common during slow-wave sleep, more than 50% of the bursting originated from 14% of the LGN cells. Bursting during sleep was largely restricted to episodes lasting 1–5 s, with ∼47% of these episodes being rhythmic and in the delta frequency range (0.5–4 Hz). In wakefulness, although visual stimulation accounted for the greatest number of bursts, it was still a small fraction of the total response (4%, 742 bursts/17,744 cycles in 93 cells). We identified two variables that appeared to influence burst probability: size of the visual stimuli used to elicit responses and behavioral state. Increased stimulus size increased burst probability. We attribute this to the increased influence large stimuli have on a cell's inhibitory mechanisms. As with sleep, a large fraction of bursting originated from a small number of cells. During visual stimulation, 50% of bursting was generated by 9% of neurons. Increased vigilance was negatively correlated with burst probability. Visual stimuli presented during active fixation (i.e., when the animal must fixate on an overt fixation point) were less likely to produce bursting, than when the same visual stimuli were presented but no fixation point present (“passive” fixation). Such observations suggest that even brief departures from attentive states can hyperpolarize neurons sufficiently to de-inactivate the burst mechanism. Our results provide a new view of the temporal structure of bursting during slow-wave sleep; one that supports episodic rhythmic activity in the intact animal. In addition, because bursting could be tied to specific conditions within wakefulness, we suggest that bursting has a specific function within that state.
Short-Latency Disparity Vergence in Humans
Authors: Busettini C. , Fitzgibbon E. J. , Miles F. A.
Published: December 2017

Eye movement recordings from humans indicated that brief exposures (200 ms) to horizontal disparity steps applied to large random-dot patterns elicit horizontal vergence at short latencies (80.9 ± 3.9 ms, mean ± SD; n = 7). Disparity tuning curves, describing the dependence of the initial vergence responses (measured over the period 90–157 ms after the step) on the magnitude of the steps, resembled the derivative of a Gaussian, with nonzero asymptotes and a roughly linear servo region that extended only a degree or two on either side of zero disparity. Responses showed transient postsaccadic enhancement: disparity steps applied in the immediate wake of saccadic eye movements yielded higher vergence accelerations than did the same steps applied some time later (mean time constant of the decay, 200 ms). This enhancement seemed to be dependent, at least in part, on the visual reafference associated with the prior saccade because similar enhancement was observed when the disparity steps were applied in the wake of saccadelike shifts of the textured pattern. Vertical vergence responses to vertical disparity steps were qualitatively similar: latencies were longer (on average, by 3 ms), disparity tuning curves had the same general form but were narrower (by ≈20%), and their peak-to-peak amplitudes were smaller (by ≈70%). Initial vergence responses usually had directional errors (orthogonal components) with a very systematic dependence on step size that often approximated an exponential decay to a nonzero asymptote (mean space constant ± SD, 1.18 ± 0.66°). Based on the asymptotes of these orthogonal responses, horizontal errors (with vertical steps) were on average more than three times greater than vertical errors (with horizontal steps). Disparity steps >7° generated “default” responses that were independent of the direction of the step, idiosyncratic, and generally had both horizontal and vertical components. We suggest that the responses depend on detectors that sense local disparity matches, and that orthogonal and “default” responses result from globally “false” matches. Recordings from three monkeys, using identical disparity stimuli, confirmed that monkeys also show short-latency disparity vergence responses (latency ≈25 ms shorter than that of humans), and further indicated that these responses show all of the major features seen in humans, the differences between the two species being solely quantitative. Based on these data and those of others implying that foveal images normally take precedence, we suggest that the mechanisms under study here ordinarily serve to correct small vergence errors, automatically, especially after saccades.
Encoding of Different Aspects of Afferent Activities by Two Types of Cells in the Corpus Glomerulosum of a Teleost Brain
Authors: Tsutsui Hidekazu , Yamamoto Naoyuki , Ito Hironobu , Oka Yoshitaka
Published: December 2017

The corpus glomerulosum (CG) is an expansive nucleus in acanthopterigian teleosts that has been suggested to be involved in vision-related information processing and the control of the hypothalamic function. The CG has only two types of constituent cells, the large cell and the small cell, and well-defined afferent/efferent fiber connections. One of the three types of teleostean CG, type III has additional outstanding morphological characters: clearly laminated organization and giant (>50 μm in diameter) tips of postsynaptic dendrites. Although such histological architecture is potentially advantageous for the study of information processing in a brain nucleus based on the physiological properties of identified cells and synapses, previous studies on the CG have been limited to anatomy. In this study, we developed a slice preparation of the type III CG in a teleost, Stephanoplepis cirrhifer, and studied the morphology and physiology of individual cells and synaptic transmission by means of dendritic intracellular and somatic whole cell recordings. The characteristic morphology of the two types of cells was revealed by intracellular staining. While both of them received similar glutamatergic and GABAergic projections from the nucleus corticalis mediated by AMPA, N-methyl-d-aspartate, and GABAA receptors, they showed quite distinctive firing properties and postsynaptic responses with current injection and synaptic inputs: the large cell fired a single spike, and the small cell fired a spike train whose frequency was dependent on the stimulus intensity. Furthermore, the large cell showed low-pass temporal filtering properties with paired stimuli. These results suggest that the large cell and the small cell may encode different aspects of the corticalis activities.
Synaptic and Nonsynaptic Contributions to Giant IPSPs and Ectopic Spikes Induced by 4-Aminopyridine in the Hippocampus In Vitro
Authors: Traub Roger D. , Bibbig Rea , Piechotta Antje , Draguhn Reas , Schmitz Dietmar
Published: December 2017

Hippocampal slices bathed in 4-aminopyridine (4-AP, ≤200 μM) exhibit 1) spontaneous large inhibitory postsynaptic potentials (IPSPs) in pyramidal cells, which occur without the necessity of fast glutamatergic receptors, and which hence are presumed to arise from coordinated firing in populations of interneurons; 2) spikes of variable amplitude, presumed to be of antidromic origin, in some pyramidal cells during the large IPSP; 3) bursts of action potentials in selected populations of interneurons, occurring independently of fast glutamatergic and of GABAAreceptors. We have used neuron pairs, and a large network model (3,072 pyramidal cells, 384 interneurons), to examine how these phenomena might be inter-related. Network bursts in electrically coupled interneurons have previously been shown to be possible with dendritic gap junctions, when the dendrites were capable of spike initiation, and when action potentials could cross from cell to cell via gap junctions; recent experimental data showing that dendritic gap junctions between cortical interneurons lead to coupling potentials of only about 0.5 mV argue against this mechanism, however. We now show that axonal gap junctions between interneurons could also lead to network bursts; this concept is consistent with the occurrence of spikelets and partial spikes in at least some interneurons in 4-AP. In our model, spontaneous antidromic action potentials can induce spikelets and action potentials in principal cells during the large IPSP. The probability of observing this type of activity increases significantly when axonal gap junctions also exist between pyramidal cells. Sufficient antidromic activity in the model can lead to epileptiform bursts, independent of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-d-aspartate (NMDA) receptors, in some principal cells, preceded by IPSPs and spikelets. The model predicts that gap junction blockers should suppress large IPSPs observed in 4-AP and should also reduce the probability of observing antidromic activity, or bursting, in pyramidal cells. Experiments show that, indeed, the gap junction blocking compound carbenoxolone does suppress spontaneous large IPSCs, occurring in 4-AP plus ionotropic glutamate blockers, together with a GABAB receptor blocker; carbenoxolone also suppresses large, fast inward currents, corresponding to ectopic spikes, which occur in 4-AP. Carbenoxolone does not suppress large depolarizing IPSPs induced by tetanic stimulation. We conclude that in 4-AP, axonal gap junctions could, at least in principle, account in part for both the large IPSPs, and for the antidromic activity in pyramidal neurons.
Representation of Pleasant and Aversive Taste in the Human Brain
Authors: O'Doherty J. , Rolls E. T. , Francis S. , Bowtell R. , McGlone F.
Published: December 2017

In this study, the representation of taste in the orbitofrontal cortex was investigated to determine whether or not a pleasant and an aversive taste have distinct or overlapping representations in this region. The pleasant stimulus used was sweet taste (1 M glucose), and the unpleasant stimulus was salt taste (0.1 M NaCl). We used an on/off block design in a 3T fMRI scanner with a tasteless solution delivered in the offperiod to control for somatosensory or swallowing-related effects. It was found that parts of the orbitofrontal cortex were activated ( P < 0.005 corrected) by glucose (in 6/7 subjects) and by salt (in 6/7 subjects). In the group analysis, separate areas of the orbitofrontal cortex were found to be activated by pleasant and aversive tastes. The involvement of the amygdala in the representation of pleasant as well as aversive tastes was also investigated. The amygdala was activated (region of interest analysis, P< 0.025 corrected) by the pleasant taste of glucose (5/7 subjects) as well as by the aversive taste of salt (4/7 subjects). Activation by both stimuli was also found in the frontal opercular/insular (primary) taste cortex. We conclude that the orbitofrontal cortex is involved in processing tastes that have both positive and negative affective valence and that different areas of the orbitofrontal cortex may be activated by pleasant and unpleasant tastes. We also conclude that the amygdala is activated not only by an affectively unpleasant taste, but also by a taste that is affectively pleasant, thus providing evidence that the amygdala is involved in effects produced by positively affective as well as by negatively affective stimuli.
Somatosensory Input to Auditory Association Cortex in the Macaque Monkey
Authors: Schroeder Charles E. , Lindsley Robert W. , Specht Colleen , Marcovici Alvin , Smiley John F. , Javitt Daniel C.
Published: December 2017

We investigated the convergence of somatosensory and auditory inputs in within subregions of macaque auditory cortex. Laminar current source density and multiunit activity profiles were sampled with linear array multielectrodes during penetrations of the posterior superior temporal plane in three macaque monkeys. At each recording site, auditory responses to binaural clicks, pure tones, and band-passed noise, all presented by earphones, were compared with somatosensory responses evoked by contralateral median nerve stimulation. Subjects were awake but were not required to discriminate the stimuli. Borders between A1 and surrounding belt regions were identified by mapping best frequency and stimulus preferences and by subsequent histological analysis. Regions immediately caudomedial to A1 had robust somatosensory responses co-represented with auditory responses. In these regions, both somatosensory and auditory response profiles had “feedforward” patterns; initial excitation beginning in Lamina 4 and spreading to extragranular laminae. Auditory and somatosensory responses displayed a high degree of temporal overlap. Anatomical reconstruction indicated that the somatosensory input region includes, but may not be restricted to, the caudomedial auditory association cortex. As was earlier reported for this region, auditory frequency tuning curves were broad and band-passed noise responses were larger than pure tone responses. No somatosensory responses were observed in A1. These findings suggest a potential neural substrate for multisensory integration at an early stage of auditory cortical processing.
Mechanism for Increased Hippocampal Synaptic Strength Following Differential Experience
Authors: Foster T. C. , Dumas T. C.
Published: December 2017

Exposure to novel environments or behavioral training is associated with increased strength at hippocampal synapses. The present study employed quantal analysis techniques to examine the mechanism supporting changes in synaptic transmission that occur following differential behavioral experience. Measures of CA1 synaptic strength were obtained from hippocampal slices of rats exposed to novel environments or maintained in individual cages. The input/output (I/O) curve of extracellularly recorded population excitatory postsynaptic potentials (EPSPs) increased for animals exposed to enrichment. The amplitude of the synaptic response of the field potential was related to the fiber potential amplitude and the paired-pulse ratio, however, these measures were not altered by differential experience. Estimates of biophysical parameters of transmission were determined for intracellularly recorded unitary responses of CA1 pyramidal cells. Enrichment was associated with an increase in the mean unitary synaptic response, an increase in quantal size, and a trend for decreased input resistance and reduction in the stimulation threshold to elicit a unitary response. Paired-pulse facilitation, the percent of response failures, coefficient of variance, and estimates of quantal content were not altered by experience but correlated well with the mean unitary response amplitude. The results suggest that baseline synaptic strength is determined, to a large extent, by presynaptic release mechanisms. However, increased synaptic transmission following environmental enrichment is likely due to an increase in the number or efficacy of receptors at some synapses and the emergence of functional synaptic contacts between previously unconnected CA3 and CA1 cells.
Effects of Spike Parameters and Neuromodulators on Action Potential Waveform-Induced Calcium Entry Into Pyramidal Neurons
Authors: Stewart Ansalan E. , Foehring Robert C.
Published: December 2017

Neocortical pyramidal neurons express several different calcium channel types. Previous studies with square voltage steps have found modest biophysical differences between these calcium channel types as well as differences in their modulation by transmitters. We used acutely dissociated neocortical pyramidal neurons to test whether this diversity extends to different activation by physiological stimuli. We conclude that 1) peak amplitude, latency to peak, and the total charge entry for the Ca2+ channel current is dependent on the shape of the mock action potential waveforms (APWs). 2) The percent contribution of the five high-voltage-activated currents to the whole cell current was not altered by using an APW as opposed to a voltage step to elicit the current. 3) The identity of the charge carrier affects the amplitude and decay of the whole cell current. With Ca2+, there was a greater contribution of T-type current to the whole cell current. 4) Total Ba2+ charge entry is linearly dependent on the number of spikes in the stimulating waveform and relatively insensitive to spike frequency. 5) Current decay was greatest with Ca2+ as the charge carrier and with minimal internal chelation. 6) Voltage-dependent neurotransmitter-mediated modulations can be reversed by multiple spikes. The extent of the reversal is dependent on the number of spikes in the stimulating waveform. Thus the neuronal activity pattern can determine the effectiveness of voltage-dependent and -independent modulatory pathways in neocortical pyramidal neurons.
High-Pass Filtering of Corticothalamic Activity by Neuromodulators Released in the Thalamus During Arousal: In Vitro and In Vivo
Authors: Castro-Alamancos Manuel A. , Calcagnotto Maria E.
Published: December 2017

The thalamus is the principal relay station of sensory information to the neocortex. In return, the neocortex sends a massive feedback projection back to the thalamus. The thalamus also receives neuromodulatory inputs from the brain stem reticular formation, which is vigorously activated during arousal. We investigated the effects of two neuromodulators, acetylcholine and norepinephrine, on corticothalamic responses in vitro and in vivo. Results from rodent slices in vitro showed that acetylcholine and norepinephrine depress the efficacy of corticothalamic synapses while enhancing their frequency-dependent facilitation. This produces a stronger depression of low-frequency responses than of high-frequency responses. The effects of acetylcholine and norepinephrine were mimicked by muscarinic and α2-adrenergic receptor agonists and blocked by muscarinic and α-adrenergic antagonists, respectively. Stimulation of the brain stem reticular formation in vivo also strongly depressed corticothalamic responses. The suppression was very strong for low-frequency responses, which do not produce synaptic facilitation, but absent for high-frequency corticothalamic responses. As in vitro, application of muscarinic and α-adrenergic antagonists into the thalamus in vivo abolished the suppression of corticothalamic responses induced by stimulating the reticular formation. In conclusion, cholinergic and noradrenergic activation during arousal high-pass filters corticothalamic activity. Thus, during arousal only high-frequency inputs from the neocortex are allowed to reach the thalamus. Neuromodulators acting on corticothalamic synapses gate the flow of cortical activity to the thalamus as dictated by behavioral state.
Hippocampal Synaptic Transmission and Plasticity Are Preserved in Myosin Va Mutant Mice
Authors: Schnell Eric , Nicoll Roger A.
Published: December 2017

Recent studies have identified myosin Va as an organelle motor that may have important functions in neurons. Abundantly expressed at the hippocampal postsynaptic density, it interacts with protein complexes involved in synaptic plasticity. It is also located in presynaptic terminals and may function to recruit vesicles in the reserve pool to the active zone. Dilute-lethal mice are spontaneous myosin Va mutants and have severe neurological symptoms. We studied hippocampal physiology at CA3-CA1 excitatory synapses in dilute-lethal mutant mice to test the hypothesis that myosin Va plays a role in pre- or postsynaptic elements of synaptic transmission. In all assays performed, the mutant synapses appeared to be functioning normally, both pre- and postsynaptically. These data suggest that myosin Va is not essential for the synaptic release machinery, postsynaptic receptor composition, or plasticity at this synapse, but does not exclude significant roles for myosin Va in other cell types nor potential compensation by other myosin V isoforms.
Mouse Taste Cells With Glialike Membrane Properties
Authors: Bigiani Albertino
Published: December 2017

Taste buds are sensory structures made up by tightly packed, specialized epithelial cells called taste cells. Taste cells are functionally heterogeneous, and a large proportion of them fire action potentials during chemotransduction. In view of the narrow intercellular spaces within the taste bud, it is expected that the ionic composition of the extracellular fluid surrounding taste cells may be altered significantly by activity. This consideration has led to postulate the existence of glialike cells that could control the microenvironment in taste buds. However, the functional identification of such cells has been so far elusive. By using the patch-clamp technique in voltage-clamp conditions, I identified a new type of cells in the taste buds of the mouse vallate papilla. These cells represented about 30% of cells patched in taste buds and were characterized by a large leakage current. Accordingly, I named them “Leaky” cells. The leakage current was carried by K+, and was blocked by Ba2+ but not by tetraethylammonium (TEA). Other taste cells, such as those possessing voltage-gated Na+ currents and thought to be chemosensory in function, did not express any sizeable leakage current. Consistent with the presence of a leakage conductance, Leaky cells had a low input resistance (∼0.25 GΩ). In addition, their zero-current (“resting”) potential was close to the equilibrium potential for potassium ions. The electrophysiological analysis of the membrane currents remaining after pharmacological block by Ba2+ revealed that Leaky cells also possessed a Cl− conductance. However, in resting conditions the membrane of these cells was about 60 times more permeable to K+ than to Cl−. The resting potassium conductance in Leaky cells could be involved in dissipating rapidly the increase in extracellular K+ during action potential discharge in chemosensory cells. Thus Leaky cells might represent glialike elements in taste buds. These findings support a model in which specific cells control the chemical composition of intercellular fluid in taste buds.
Nonlinear Behavior of Sinusoidally Forced Pyloric Pacemaker Neurons
Authors: Szűcs Attila , Elson Robert C. , Rabinovich Michail I. , Abarbanel Henry D. I. , Selverston Allen I.
Published: December 2017

Periodic current forcing was used to investigate the intrinsic dynamics of a small group of electrically coupled neurons in the pyloric central pattern generator (CPG) of the lobster. This group contains three neurons, namely the two pyloric dilator (PD) motoneurons and the anterior burster (AB) interneuron. Intracellular current injection, using sinusoidal waveforms of varying amplitude and frequency, was applied in three configurations of the pacemaker neurons: 1) the complete pacemaker group, 2) the two PDs without the AB, and 3) the AB neuron isolated from the PDs. Depending on the frequency and amplitude of the injected current, the intact pacemaker group exhibited a wide variety of nonlinear behaviors, including synchronization to the forcing, quasiperiodicity, and complex dynamics. In contrast, a single, broad 1:1 entrainment zone characterized the response of the PD neurons when isolated from the main pacemaker neuron AB. The isolated AB responded to periodic forcing in a manner similar to the complete pacemaker group, but with wider zones of synchronization. We have built an analog electronic circuit as an implementation of a modified Hindmarsh-Rose model for simulating the membrane potential activity of pyloric neurons. We subjected this electronic model neuron to the same periodic forcing as used in the biological experiments. This four-dimensional electronic model neuron reproduced the autonomous oscillatory firing patterns of biological pyloric pacemaker neurons, and it expressed the same stationary nonlinear responses to periodic forcing as its biological counterparts. This adds to our confidence in the model. These results strongly support the idea that the intact pyloric pacemaker group acts as a uniform low-dimensional deterministic nonlinear oscillator, and the regular pyloric oscillation is the outcome of cooperative behavior of strongly coupled neurons, having different dynamical and biophysical properties when isolated.
Neural Processing of Gravito-Inertial Cues in Humans. II. Influence of the Semicircular Canals During Eccentric Rotation
Authors: Merfeld D. M. , Zupan L. H. , Gifford C. A.
Published: December 2017

All linear accelerometers, including the otolith organs, respond equivalently to gravity and linear acceleration. To investigate how the nervous system resolves this ambiguity, we measured perceived roll tilt and reflexive eye movements in humans in the dark using two different centrifugation motion paradigms (fixed radius and variable radius) combined with two different subject orientations (facing-motion and back-to-motion). In the fixed radius trials, the radius at which the subject was seated was held constant while the rotation speed was changed to yield changes in the centrifugal force. In variable radius trials, the rotation speed was held constant while the radius was varied to yield a centrifugal force that nearly duplicated that measured during the fixed radius condition. The total gravito-inertial force (GIF) measured by the otolith organs was nearly identical in the two paradigms; the primary difference was the presence (fixed radius) or absence (variable radius) of yaw rotational cues. We found that the yaw rotational cues had a large statistically significant effect on the time course of perceived tilt, demonstrating that yaw rotational cues contribute substantially to the neural processing of roll tilt. We also found that the orientation of the subject relative to the centripetal acceleration had a dramatic influence on the eye movements measured during fixed radius centrifugation. Specifically, the horizontal vestibuloocular reflex (VOR) measured in our human subjects was always greater when the subject faced the direction of motion than when the subjects had their backs toward the motion during fixed radius rotation. This difference was consistent with the presence of a horizontal translational VOR response induced by the centripetal acceleration. Most importantly, by comparing the perceptual tilt responses to the eye movement responses, we found that the translational VOR component decayed as the subjective tilt indication aligned with the tilt of the GIF. This was true for both the fixed radius and variable radius conditions even though the time course of the responses was significantly different for these two conditions. These findings are consistent with the hypothesis that the nervous system resolves the ambiguous measurements of GIF into neural estimates of gravity and linear acceleration. More generally, these findings are consistent with the hypothesis that the nervous system uses internal models to process and interpret sensory motor cues.
Slow and Fast (Gamma) Neuronal Oscillations in the Perirhinal Cortex and Lateral Amygdala
Authors: Collins Dawn R. , Pelletier J. Guillaume , Paré Denis
Published: December 2017

Most lesion studies emphasize the distinct contributions of the amygdala and perirhinal cortex to memory. Yet, the presence of strong reciprocal excitatory projections between these two structures suggests that they are functionally coupled. To gain some insight into this issue, the present study examined whether the close anatomical ties existing between perirhinal and lateral amygdala (LA) neurons are expressed in their spontaneous activity. To this end, multiple simultaneous recordings of single unit discharges and local field potentials were performed in the LA and perirhinal cortex in ketamine-xylazine anesthetized cats. The perirhinal cortex and LA exhibited a similar pattern of spontaneous activity. Recordings at both sites were dominated by a slow focal oscillation at 1 Hz onto which was superimposed a faster rhythm (≈30 Hz) whose amplitude fluctuated cyclically. Computing crosscorrelograms between focal waves recorded simultaneously in the perirhinal cortex and LA revealed a close relationship between their spontaneous activity. Even when recording sites were separated by as much as 8 mm, the slow focal oscillation remained highly correlated ( r ≥ 0.7). In contrast, the correlation between fast oscillations was usually lower ( r ≈ 0.3). Perievent histograms of neuronal discharges revealed that the firing probability of most LA and perirhinal neurons increased during the depth-negative component of the slow oscillation. In addition, respectively, 47 and 64% of LA and perirhinal neurons exhibited a significant modulation of firing probability in relation to the fast oscillations. Finally, crosscorrelating unit discharges simultaneously recorded in the LA and perirhinal cortex confirmed the presence of phase-related oscillatory events in both structures. In summary, our results suggest that the interconnections existing between the perirhinal cortex and LA can support the genesis of coherent neuronal activities at various frequencies. These results imply that cooperative interactions must be taking place between these structures.
Spatial Distribution and Subunit Composition of GABAAReceptors in the Inferior Olivary Nucleus
Authors: Devor Anna , Fritschy Jean-Marc , Yarom Yosef
Published: December 2017

GABAergic inhibitory feedback from the cerebellum onto the inferior olivary (IO) nucleus plays an important role in olivo-cerebellar function. In this study we characterized the physiology, subunit composition, and spatial distribution of γ-aminobutyric acid-A (GABAA) receptors in the IO nucleus. Using brain stem slices, we identified two types of IO neuron response to local pressure application of GABA, depending on the site of application: a slow desensitizing response at the soma and a fast desensitizing response at the dendrites. The dendritic response had a more negative reversal potential than did the somatic response, which confirmed their spatial origin. Both responses showed voltage dependence characterized by an abrupt decrease in conductance at negative potentials. Interestingly, this change in conductance occurred in the range of potentials wherein subthreshold membrane potential oscillations usually occur in IO neurons. Immunostaining IO sections with antibodies for GABAA receptor subunits α1, α2, α3, α5, β2/3, and γ2 and against the postsynaptic anchoring protein gephyrin complemented the electrophysiological observation by showing a differential distribution of GABAA receptor subtypes in IO neurons. A receptor complex containing α2β2/3γ2 subunits is clustered with gephyrin at presumptive synaptic sites, predominantly on distal dendrites. In addition, diffuse α3, β2/3, and γ2 subunit staining on somata and in the neuropil presumably represents extrasynaptic receptors. Combining electrophysiology with immunocytochemistry, we concluded that α2β2/3γ2 synaptic receptors generated the fast desensitizing (dendritic) response at synaptic sites whereas the slow desensitizing (somatic) response was generated by extrasynaptic α3β2/3γ2 receptors.
Time-Resolved fMRI of Activation Patterns in M1 and SMA During Complex Voluntary Movement
Authors: Weilke Florian , Spiegel Sabine , Boecker Henning , von Einsiedel Helga Gräfin , Conrad Bastian , Schwaiger Markus , Erhard Peter
Published: December 2017

The aim of this study was to use time-resolved functional magnetic resonance imaging (fMRI) to investigate temporal differences in the activation of the supplementary motor area (SMA) and the primary motor cortex (M1). We report data from eight human volunteers who underwent fMRI examinations in a 1.5T Philips Gyroscan ACS-NT MRI scanner. While wearing a contact glove, subjects executed a complex automated sequence of finger movements either spontaneously or in response to external auditory cues. Based on the result of a functional scout scan, a single slice that included the M1 and the SMA was selected for image acquisition (echo planar imaging, repetition time 100 ms, echo time 50 ms, 64 × 64 matrix, 1,000 images). Data were analyzed with a shifting cross-correlation approach using the STIMULATE program and in-house programs written in Interactive Data Language (IDL™). Time-course data were generated for regions of interest in the M1 as well as in the rostral and caudal SMA. Mean time between onset of the finger movement sequence and half-maximum of the signal change in M1 was 3.6 s for the externally cued execution (SD 0.5) and 3.5 s for the spontaneous execution (SD 0.6). Activation in the rostral section of the SMA occurred 0.7 s earlier than it did in the M1 during the externally cued execution and 2.0 s earlier during the spontaneous execution, a difference significant at the P < 0.01 level. Our results indicate that rostral SMA activation precedes M1 activation by varying time intervals in the sub-second range that are determined by the mode of movement initialization. By applying a paradigm that exerts a differential influence on temporal activation, we could ensure that the observed timing differences were not the result of differences in hemodynamic response function.
Electrophysiological Characterization of “Giant” Cells in Stratum Radiatum of the CA3 Hippocampal Region
Authors: Savić Nataša , Sciancalepore Marina
Published: December 2017

Whole cell patch-clamp recording and intracellular staining with biocytin allowed the morphological and electrophysiological characterization of “giant” cells, studied in stratum (st.) radiatum of the CA3 region in 17- to 21-day-old rat hippocampal slices. These neurons had extensive dendritic arborization, a triangular soma, and a bipolar vertical orientation with axons directed to the pyramidal layer or extended into the st. oriens. Giant cells had significantly higher input resistance and shorter action potentials compared with CA3 pyramidal cells. Evoked action potentials were typically followed by an afterdepolarizing potential (ADP). During depolarizing current injection, most (80%) of recorded giant cells displayed a regular firing pattern (maximum steady-state firing rate, ∼30 Hz) characterized by a modest early accommodation, whereas irregular firing was observed in the remaining 20% of giant cells. Hyperpolarizing current pulses induced a slow inward rectification of the electrotonic voltage responses, blocked by 2 mM external Cs+. N-methyl-d-aspartate (NMDA) and non-NMDA–mediated excitatory postsynaptic currents (EPSCs) measured under voltage clamp were distinguished on the basis of their voltage dependence and sensitivity to specific NMDA and non-NMDA glutamate receptor blockers. Non-NMDA EPSCs possessed a linear current-voltage relationship. EPSCs elicited by st. lucidum stimulation were reversibly reduced (mean, 23%) by the group II metabotropic glutamate receptor agonist (2S, 1′R, 2′R, 3′R)-2-(2,3-dicarboxyl-cyclopropyl)-glycine (DCG-IV, 1 μM). GABAA-mediated postsynaptic currents were subject to paired-pulse depression that was inhibited by the GABAB antagonist CGP 55845A (5 μM). We conclude that CA3 giant cells represent a particular class of hippocampal neuron located in st. radiatum that shares only some morphological and physiological properties with principal cells.
Enhanced Temporal Stability of Cholinergic Hippocampal Gamma Oscillations Following Respiratory Alkalosis In Vitro
Authors: Stenkamp Kerstin , Palva J. Matias , Uusisaari Marylka , Schuchmann Sebastian , Schmitz Dietmar , Heinemann Uwe , Kaila Kai
Published: December 2017

The decrease in brain CO2 partial pressure (pCO2) that takes place both during voluntary and during pathological hyperventilation is known to induce gross alterations in cortical functions that lead to subjective sensations and altered states of consciousness. The mechanisms that mediate the effects of the decrease in pCO2 at the neuronal network level are largely unexplored. In the present work, the modulation of gamma oscillations by hypocapnia was studied in rat hippocampal slices. Field potential oscillations were induced by the cholinergic agonist carbachol under an N-methyl-D-aspartate (NMDA)-receptor blockade and were recorded in the dendritic layer of the CA3 region with parallel measurements of changes in interstitial and intraneuronal pH (pHo and pHi, respectively). Hypocapnia from 5 to 1% CO2 led to a stable monophasic increase of 0.5 and 0.2 units in pHo and pHi, respectively. The mean oscillation frequency increased slightly but significantly from 32 to 34 Hz and the mean gamma-band amplitude (20 to 80 Hz) decreased by 20%. Hypocapnia induced a dramatic enhancement of the temporal stability of the oscillations, as was indicated by a two-fold increase in the exponential decay time constant fitted to the autocorrelogram. A rise in pHi evoked by the weak base trimethylamine (TriMA) was associated with a slight increase in oscillation frequency (37 to 39 Hz) and a decrease in amplitude (30%). Temporal stability, on the other hand, was decreased by TriMA, which suggests that its enhancement in 1% CO2 was related to the rise in pHo. In 1% CO2, the decay-time constant of the evoked monosynaptic pyramidal inhibitory postsynaptic current (IPSC) was unaltered but its amplitude was enhanced. This increase in IPSC amplitude seems to significantly contribute to the enhancement of temporal stability because the enhancement was almost fully reversed by a low concentration of bicuculline. These results suggest that changes in brain pCO2 can have a strong influence on the temporal modulation of gamma rhythms.
Post-Episode Depression of GABAergic Transmission in Spinal Neurons of the Chick Embryo
Authors: Chub Nikolai , O'Donovan Michael J.
Published: December 2017

Whole cell recordings were obtained from ventral horn neurons in spontaneously active spinal cords isolated from the chick embryo [ embryonic days 10 to 11 ( E10–E11)] to examine the post-episode depression of GABAergic transmission. Spontaneous activity occurred as recurrent, rhythmic episodes approximately 60 s in duration with 10- to 15-min quiescent inter-episode intervals. Current-clamp recording revealed that episodes were followed by a transient hyperpolarization (7 ± 1.2 mV, mean ± SE), which dissipated as a slow (0.5–1 mV/min) depolarization until the next episode. Local application of bicuculline 8 min after an episode hyperpolarized spinal neurons by 6 ± 0.8 mV and increased their input resistance by 13%, suggesting the involvement of GABAergic transmission. Gramicidin perforated-patch recordings showed that the GABAa reversal potential was above rest potential ( E GABAa = −29 ± 3 mV) and allowed estimation of the physiological intracellular [Cl−] = 50 mM. In whole cell configuration (with physiological electrode [Cl−]), two distinct types of endogenous GABAergic currents ( I GABAa) were found during the inter-episode interval. The first comprised TTX-resistant, asynchronous miniature postsynaptic currents (mPSCs), an indicator of quantal GABA release (up to 42% of total mPSCs). The second (tonic I GABAa) was complimentary to the slow membrane depolarization and may arise from persistent activation of extrasynaptic GABAa receptors. We estimate that approximately 10 postsynaptic channels are activated by a single quantum of GABA release during an mPSC and that about 30 extrasynaptic GABAa channels are required for generation of the tonic I GABAa in ventral horn neurons. We investigated the post-episode depression of I GABAa by local application of GABA or isoguvacine (100 μM, for 10–30 s) applied before and after an episode at holding potentials ( V hold) −60 mV. The amplitude of the evoked I GABA was compared after clamping the cell during the episode at one of three different V hold: −60 mV, below E GABAa resulting in Cl− efflux; −30 mV, close to E GABAa with minimal Cl− flux; and 0 mV, above E GABAa resulting in Cl− influx during the episode. The amplitude of the evoked I GABA changed according to the direction of Cl− flux during the episode: at −60 mV a 41% decrease, at −30 mV a 4% reduction, and at 0 mV a 19% increase. These post-episode changes were accompanied by shifts of E GABAa of −10, −1.2, and +7 mV, respectively. We conclude that redistribution of intracellular [Cl−] during spontaneous episodes is likely to be an important postsynaptic mechanism involved in the post-episode depression of GABAergic transmission in chick embryo spinal neurons.
Role of Mammalian Auditory Cortex in the Perception of Elementary Sound Properties
Authors: Talwar Sanjiv K. , Musial Pawel G. , Gerstein George L.
Published: December 2017

Studies in several mammalian species have demonstrated that bilateral ablations of the auditory cortex have little effect on simple sound intensity and frequency-based behaviors. In the rat, for example, early experiments have shown that auditory ablations result in virtually no effect on the rat's ability to either detect tones or discriminate frequencies. Such lesion experiments, however, typically examine an animal's performance some time after recovery from ablation surgery. As such, they demonstrate that the cortex is not essential for simple auditory behaviors in the long run. Our study further explores the role of cortex in basic auditory perception by examining whether the cortex is normally involved in these behaviors. In these experiments we reversibly inactivated the rat primary auditory cortex (AI) using the GABA agonist muscimol, while the animals performed a simple auditory task. At the same time we monitored the rat's auditory activity by recording auditory evoked potentials (AEP) from the cortical surface. In contrast to lesion studies, the rapid time course of these experimental conditions preclude reorganization of the auditory system that might otherwise compensate for the loss of cortical processing. Soon after bilateral muscimol application to their AI region, our rats exhibited an acute and profound inability to detect tones. After a few hours this state was followed by a gradual recovery of normal hearing, first of tone detection and, much later, of the ability to discriminate frequencies. Surface muscimol application, at the same time, drastically altered the normal rat AEP. Some of the normal AEP components vanished nearly instantaneously to unveil an underlying waveform, whose size was related to the severity of accompanying behavioral deficits. These results strongly suggest that the cortex is directly involved in basic acoustic processing. Along with observations from accompanying multiunit experiments that related the AEP to AI neuronal activity, our results suggest that a critical amount of activity in the auditory cortex is necessary for normal hearing. It is likely that the involvement of the cortex in simple auditory perceptions has hitherto not been clearly understood because of underlying recovery processes that, in the long-term, safeguard fundamental auditory abilities after cortical injury.