bluuebunny/crossref_metadata_2025 huggingface dataset:
Strain-Dependent Adsorption of Pseudomonas aeruginosa-Derived Adhesin-Like Peptides at Abiotic Surfaces
Authors: Yang Yu , Schwiderek Sabrina , Grundmeier Guido , Keller Adrian
Published: August 2021

Implant-associated infections are an increasingly severe burden on healthcare systems worldwide and many research activities currently focus on inhibiting microbial colonization of biomedically relevant surfaces. To obtain molecular-level understanding of the involved processes and interactions, we investigate the adsorption of synthetic adhesin-like peptide sequences derived from the type IV pili of the Pseudomonas aeruginosa strains PAK and PAO at abiotic model surfaces, i.e., Au, SiO2, and oxidized Ti. These peptides correspond to the sequences of the receptor-binding domain 128–144 of the major pilin protein, which is known to facilitate P. aeruginosa adhesion at biotic and abiotic surfaces. Using quartz crystal microbalance with dissipation monitoring (QCM-D), we find that peptide adsorption is material- as well as strain-dependent. At the Au surface, PAO(128–144) shows drastically stronger adsorption than PAK(128–144), whereas adsorption of both peptides is markedly reduced at the oxide surfaces with less drastic differences between the two sequences. These observations suggest that peptide adsorption is influenced by not only the peptide sequence, but also peptide conformation. Our results furthermore highlight the importance of molecular-level investigations to understand and ultimately control microbial colonization of surfaces.
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module
Authors: Schirmer Tilman , de Beer Tjaart A. P. , Tamegger Stefanie , Harms Alexander , Dietz Nikolaus , Dranow David M. , Edwards Thomas E. , Myler Peter J. , Phan Isabelle , Dehio Christoph
Published: August 2021

Proteins containing a FIC domain catalyze AMPylation and other post-translational modifications (PTMs). In bacteria, they are typically part of FicTA toxin-antitoxin modules that control conserved biochemical processes such as topoisomerase activity, but they have also repeatedly diversified into host-targeted virulence factors. Among these, Bartonella effector proteins (Beps) comprise a particularly diverse ensemble of FIC domains that subvert various host cellular functions. However, no comprehensive comparative analysis has been performed to infer molecular mechanisms underlying the biochemical and functional diversification of FIC domains in the vast Bep family. Here, we used X-ray crystallography, structural modelling, and phylogenetic analyses to unravel the expansion and diversification of Bep repertoires that evolved in parallel in three Bartonella lineages from a single ancestral FicTA toxin-antitoxin module. Our analysis is based on 99 non-redundant Bep sequences and nine crystal structures. Inferred from the conservation of the FIC signature motif that comprises the catalytic histidine and residues involved in substrate binding, about half of them represent AMP transferases. A quarter of Beps show a glutamate in a strategic position in the putative substrate binding pocket that would interfere with triphosphate-nucleotide binding but may allow binding of an AMPylated target for deAMPylation or another substrate to catalyze a distinct PTM. The β-hairpin flap that registers the modifiable target segment to the active site exhibits remarkable structural variability. The corresponding sequences form few well-defined groups that may recognize distinct target proteins. The binding of Beps to promiscuous FicA antitoxins is well conserved, indicating a role of the antitoxin to inhibit enzymatic activity or to serve as a chaperone for the FIC domain before translocation of the Bep into host cells. Taken together, our analysis indicates a remarkable functional plasticity of Beps that is mostly brought about by structural changes in the substrate pocket and the target dock. These findings may guide future structure–function analyses of the highly versatile FIC domains.
Phytase-Producing Rahnella aquatilis JZ-GX1 Promotes Seed Germination and Growth in Corn (Zea mays L.)
Authors: Li Gui-E , Kong Wei-Liang , Wu Xiao-Qin , Ma Shi-Bo
Published: August 2021

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1, the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.
Mechanochemical Synthesis of Rosin-Modified Montmorillonite: A Breakthrough Approach to the Next Generation of OMMT/Rubber Nanocomposites
Authors: Esmaeili Elaheh , Rounaghi Seyyed Amin , Eckert Jürgen
Published: August 2021

The current investigation presents a green mechanochemical procedure for the synthesis of a special kind of rubber-compatible organo-montmorillonite (OMMT) for use in the inner liner compound of tires. The compatibility character of the OMMT arises from the mechanochemical reaction of the raw bentonite mineral and gum rosin as some of the organic constituents of the inner liner composition. The monitoring of OMMT synthesis by various characterization techniques reveals that gum rosin gradually intercalates into the montmorillonite (MMT) galleries during milling and increases the interlayer spacing to 41.1 ± 0.5 Å. The findings confirm the simultaneous formation of single- or few-layered OMMT platelets with average sizes from the sub-micron range up to several micrometers during the milling process. The mechanical properties of the OMMT/rubber nanocomposite, such as tensile strength, tear resistance and elongation, present a good enhancement in comparison to the un-modified material. Moreover, the organo-modification of the inner liner composition also leads to a property improvement of about 50%.
The Influence of Temperature Increase on the Toxicity of Mercury Remediated Seawater Using the Nanomaterial Graphene Oxide on the Mussel Mytilus galloprovincialis
Authors: Coppola Francesca , Soares Amadeu M. V. M. , Figueira Etelvina , Pereira Eduarda , Marques Paula A. A. P. , Polese Gianluca , Freitas Rosa
Published: August 2021

Mercury (Hg) has been increasing in waters, sediments, soils and air, as a result of natural events and anthropogenic activities. In aquatic environments, especially marine systems (estuaries and lagoons), Hg is easily bioavailable and accumulated by aquatic wildlife, namely bivalves, due to their lifestyle characteristics (sedentary and filter-feeding behavior). In recent years, different approaches have been developed with the objective of removing metal(loid)s from the water, including the employment of nanomaterials. However, coastal systems and marine organisms are not exclusively challenged by pollutants but also by climate changes such as progressive temperature increment. Therefore, the present study aimed to (i) evaluate the toxicity of remediated seawater, previously contaminated by Hg (50 mg/L) and decontaminated by the use of graphene-based nanomaterials (graphene oxide (GO) functionalized with polyethyleneimine, 10 mg/L), towards the mussel Mytilus galloprovincialis; (ii) assess the influence of temperature on the toxicity of decontaminated seawater. For this, alterations observed in mussels’ metabolic capacity, oxidative and neurotoxic status, as well as histopathological injuries in gills and digestive tubules were measured. This study demonstrated that mussels exposed to Hg contaminated seawater presented higher impacts than organisms under remediated seawater. When comparing the impacts at 21 °C (present study) and 17 °C (previously published data), organisms exposed to remediated seawater at a higher temperature presented higher injuries than organisms at 17 °C. These results indicate that predicted warming conditions may negatively affect effective remediation processes, with the increasing of temperature being responsible for changes in organisms’ sensitivity to pollutants or increasing pollutants toxicity.
Ballistic Heat Transport in Nanocomposite: The Role of the Shape and Interconnection of Nanoinclusions
Authors: Desmarchelier Paul , Carré Alice , Termentzidis Konstantinos , Tanguy Anne
Published: August 2021

In this article, the effect on the vibrational and thermal properties of gradually interconnected nanoinclusions embedded in an amorphous silicon matrix is studied using molecular dynamics simulations. The nanoinclusion arrangement ranges from an aligned sphere array to an interconnected mesh of nanowires. Wave-packet simulations scanning different polarizations and frequencies reveal that the interconnection of the nanoinclusions at constant volume fraction induces a strong increase of the mean free path of high frequency phonons, but does not affect the energy diffusivity. The mean free path and energy diffusivity are then used to estimate the thermal conductivity, showing an enhancement of the effective thermal conductivity due to the existence of crystalline structural interconnections. This enhancement is dominated by the ballistic transport of phonons. Equilibrium molecular dynamics simulations confirm the tendency, although less markedly. This leads to the observation that coherent energy propagation with a moderate increase of the thermal conductivity is possible. These findings could be useful for energy harvesting applications, thermal management or for mechanical information processing.
ZnO Polymeric Composite Films for n-Decane Removal from Air Streams in a Continuous Flow NETmix Photoreactor under UVA Light
Authors: Zanrosso Crissie D. , Miranda Sandra M. , M. da Costa Filho Batuira , Espíndola Jonathan C. , Piazza Diego , J. P. Vilar Vítor , A. Lansarin Marla
Published: August 2021

Polymeric composite films have been explored for many photocatalytic applications, from water treatment to self-cleaning devices. Their properties, namely, thickness and porosity, are controlled mainly by the preparation conditions. However, little has been discussed on the effect of thickness and porosity of polymeric composite films for photocatalytic processes, especially in gas phase. In the present study, different preparation treatments of ZnO-based polymeric composite films and their effects on its performance and stability were investigated. The polymeric composites were prepared by solution mixing followed by non-solvent induced phase separation (NIPS), using poly(vinylidene fluoride) (PVDF) as the matrix and ZnO-based photocatalysts. Different wet thickness, photocatalyst mass, and treatments (e.g., using or not pore-forming agent and compatibilizer) were assessed. A low ZnO/PVDF ratio and higher wet thickness, together with the use of pore-forming agent and compatibilizer, proved to be a good strategy for increasing photocatalytic efficiency given the low agglomerate formation and high polymer transmittance. Nonetheless, the composites exhibited deactivation after several minutes of exposure. Characterization by XRD, FTIR-ATR, and SEM were carried out to further investigate the polymeric film treatments and stability. ZnO film was most likely deactivated due to zinc carbonate formation intensified by the polymer presence.
Targeting Intracellular Mycobacteria Using Nanosized Niosomes Loaded with Antibacterial Agents
Authors: Slavin Yael Nicole , Ivanova Kristina , Tang Wei-lun , Tzanov Tzanko , Li Shyh-dar , Bach Horacio
Published: August 2021

Background: Pathogenic intracellular mycobacteria are challenging to treat because of the waxy and complex cell wall characterizing the genus. Niosomes are vesicles with biomimetic cell membrane composition, which allow them to efficiently bind to the eukaryotic cells and deliver their cargo into the cytoplasm. The objective of this study was to develop a new platform based on niosomes loaded with antimicrobial agents to target intracellular mycobacteria. Nanoniosomes were fabricated and loaded with antibiotics and lignin–silver nanoparticles. The efficacy of these nanoniosomes was tested against the intracellular pathogen Mycobacterium abscessus used as a model of infection of human-derived macrophages (THP-1). The cytotoxicity and the immunological response of the agents were tested on THP-1 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the secretion of pro- and anti-inflammatory cytokines, respectively. Results: M. abscessus was susceptible to the nanoniosomes in infected THP-1 macrophages, suggesting that the nanoniosomes were internalized due to their fusion to the macrophage cellular membrane. Moreover, nanoniosomes showed no upregulation of pro-inflammatory cytokines when exposed to THP-1 macrophages. Conclusions: Nanoniosomes improved drug efficacy while decreasing toxicity and should be considered for further testing in the treatment of intracellular pathogenic mycobacteria or as a new platform for precise intracellular delivery of drugs.
Effects of Different Light Spectra on Final Biomass Production and Nutritional Quality of Two Microgreens
Authors: Toscano Stefania , Cavallaro Valeria , Ferrante Antonio , Romano Daniela , Patané Cristina
Published: August 2021

To improve microgreen yield and nutritional quality, suitable light spectra can be used. Two species—amaranth (Amaranthus tricolor L.) and turnip greens (Brassica rapa L. subsp. oleifera (DC.) Metzg)—were studied. The experiment was performed in a controlled LED environment growth chamber (day/night temperatures of 24 ± 2 °C, 16 h photoperiod, and 50/60% relative humidity). Three emission wavelengths of a light-emitting diode (LED) were adopted for microgreen lighting: (1) white LED (W); (2) blue LED (B), and (3) red LED (R); the photosynthetic photon flux densities were 200 ± 5 µmol for all light spectra. The response to light spectra was often species-specific, and the interaction effects were significant. Morphobiometric parameters were influenced by species, light, and their interaction; at harvest, in both species, the fresh weight was significantly greater under B. In amaranth, Chl a was maximized in B, whereas it did not change with light in turnip greens. Sugar content varied with the species but not with the light spectra. Nitrate content of shoots greatly varied with the species; in amaranth, more nitrates were measured in R, while no difference in turnip greens was registered for the light spectrum effect. Polyphenols were maximized under B in both species, while R depressed the polyphenol content in amaranth.
Dissecting the Genetic Basis of Flowering Time and Height Related-Traits Using Two Doubled Haploid Populations in Maize
Authors: Du Lei , Zhang Hao , Xin Wangsen , Ma Kejun , Du Dengxiang , Yu Changping , Liu Yongzhong
Published: August 2021

In the field, maize flowering time and height traits are closely linked with yield, planting density, lodging resistance, and grain fill. To explore the genetic basis of flowering time and height traits in maize, we investigated six related traits, namely, days to anthesis (AD), days to silking (SD), the anthesis–silking interval (ASI), plant height (PH), ear height (EH), and the EH/PH ratio (ER) in two locations for two years based on two doubled haploid (DH) populations. Based on the two high-density genetic linkage maps, 12 and 22 quantitative trait loci (QTL) were identified, respectively, for flowering time and height-related traits. Of these, ten QTLs had overlapping confidence intervals between the two populations and were integrated into three consensus QTLs (qFT_YZ1a, qHT_YZ5a, and qHT_YZ7a). Of these, qFT_YZ1a, conferring flowering time, is located at 221.1–277.0 Mb on chromosome 1 and explained 10.0–12.5% of the AD and SD variation, and qHT_YZ5a, conferring height traits, is located at 147.4–217.3 Mb on chromosome 5 and explained 11.6–15.3% of the PH and EH variation. These consensus QTLs, in addition to the other repeatedly detected QTLs, provide useful information for further genetic studies and variety improvements in flowering time and height-related traits.
Reactive Oxygen Species in Host Plant Are Required for an Early Defense Response against Attack of Stagonospora nodorum Berk. Necrotrophic Effectors SnTox
Authors: Veselova Svetlana , Nuzhnaya Tatyana , Burkhanova Guzel , Rumyantsev Sergey , Maksimov Igor
Published: August 2021

Reactive oxygen species (ROS) play a central role in plant immune responses. The most important virulence factors of the Stagonospora nodorum Berk. are multiple fungal necrotrophic effectors (NEs) (SnTox) that affect the redox-status and cause necrosis and/or chlorosis in wheat lines possessing dominant susceptibility genes (Snn). However, the effect of NEs on ROS generation at the early stages of infection has not been studied. We studied the early stage of infection of various wheat genotypes with S nodorum isolates -Sn4VD, SnB, and Sn9MN, carrying a different set of NE genes. Our results indicate that all three NEs of SnToxA, SnTox1, SnTox3 significantly contributed to cause disease, and the virulence of the isolates depended on their differential expression in plants (Triticum aestivum L.). The Tsn1–SnToxA, Snn1–SnTox1and Snn3–SnTox3 interactions played an important role in inhibition ROS production at the initial stage of infection. The Snn3–SnTox3 inhibited ROS production in wheat by affecting NADPH-oxidases, peroxidases, superoxide dismutase and catalase. The Tsn1–SnToxA inhibited ROS production in wheat by affecting peroxidases and catalase. The Snn1–SnTox1 inhibited the production of ROS in wheat by mainly affecting a peroxidase. Collectively, these results show that the inverse gene-for gene interactions between effector of pathogen and product of host sensitivity gene suppress the host’s own PAMP-triggered immunity pathway, resulting in NE-triggered susceptibility (NETS). These results are fundamentally changing our understanding of the development of this economical important wheat disease.
Design of a Centralized Bioenergy Unit at Comarca Lagunera, Mexico: Modeling Strategy to Optimize Bioenergy Production and Reduce Methane Emissions
Authors: Silva-González José Alberto , Hernández-De Lira Inty Omar , Rodríguez-Martínez Antonio , Ruiz-Santoyo Grace Aileen , Juárez-López Berenice , Balagurusamy Nagamani
Published: August 2021

A centralized bioenergy unit was simulated, focusing on optimizing the manure transport chain, installing a centralized biogas plant, operation costs of the process, biogas upgrading, organic fertilizer production, and economic analyses. Comarca Lagunera from northeast Mexico was chosen as a study zone due to the existing number of dairy farms and livestock population (64,000 cattle heads). Two scenarios were analyzed: The first centralized scenario consisted of selecting one unique location for the anaerobic digesters for the 16 farms; the second decentralized scenario consisted of distributing the anaerobic digesters in three locations. Optimal locations were determined using mathematical modeling. The bioenergy unit was designed to process 1600 t/day of dairy manure. Results indicated that biomethane production was a more profitable option than generating electricity with non-purified methane. The amount of biomethane production was 58,756 m3/day. Economic analysis for centralized bioenergy unit scenario showed a net production cost of USD $0.80 per kg of biomethane with a profit margin of 14.4% within 10.7 years. The decentralized bioenergy unit scenario showed a net production cost of USD $0.80 per kg of biomethane with a profit of 12.9% within 11.4 years. This study demonstrated the techno-economical and environmental feasibility for centralized and decentralized bioenergy units.
Phytochemical Screening and Antimicrobial Activity of Various Extracts of Aerial Parts of Rhanterium epapposum
Authors: Rajendrasozhan Saravanan , Moll Hani El , Snoussi Mejdi , Romeilah Ramy M. , Shalaby Emad A. , Younes Kareem M. , El-Beltagi Hossam S.
Published: August 2021

Rhanterium epapposum, native to the Arabian Peninsula, is traditionally used to cure skin infections. The objective is to screen the phytochemical content and antimicrobial activity of aqueous, methanol and 80% methanol extracts of aerial parts of R. epapposum. The phytochemical screening of aqueous, methanolic, and 80% methanol extracts of R. epapposum was conducted using gas chromatographymass spectrometry. The antimicrobial activities of the extracts were assessed by well diffusion and microdilution methods. Qualitative phytochemical analysis revealed the presence of 2-methoxy-4-vinylphenol in all three extracts, whereas ethanol, 2-methoxy-, acetate; n-hexadecanoic acid; and 2,3-butanediol are present in higher amount exclusively in the methanol, 80% methanol and aqueous extracts of the aerial parts of R. epapposum, respectively. The highest antibacterial activity was shown by the aqueous extract S. aureus, P. aeruginosa, E. cloacae, and K. pneumoniae, methanolic extract against S. aureus, methicillin-resistant S. aureus, and E. coli, and 80% methanol extract against S. epidermidis, and S. paucimobilis. Interestingly, 80% methanol extracts showed the highest antifungal activity against C. albicans, C. guillermondii, C. vaginalis, C. utilis, and C. tropicalis. The aerial parts of R. epapposum showed broad-spread antimicrobial activity against bacteria and fungi. Especially, the 80% methanol extract showed potent antifungal activity against all the tested fungal strains.
Removal of Dissolved Oxygen from Water by Nitrogen Stripping Coupled with Vacuum Degassing in a Rotor–Stator Reactor
Authors: Zhao Zemeng , Liu Zhibang , Xiang Yang , Arowo Moses , Shao Lei
Published: August 2021

Oxygen is a harmful substance in many processes because it can bring out corrosion and oxidation of food. This study aimed to enhance the removal of dissolved oxygen (DO) from water by employing a novel rotor–stator reactor (RSR). The effectiveness of the nitrogen stripping coupled with vacuum degassing technique for the removal of DO from water in the RSR was investigated. The deoxygenation efficiency (η) and the mass transfer coefficient (KLa) were determined under various operating conditions for the rotational speed, liquid volumetric flow rate, gas volumetric flow rate, and vacuum degree. The nitrogen stripping coupled with vacuum degassing technique achieved values for η and KLa of 97.34% and 0.0882 s−1, respectively, which are much higher than those achieved with the vacuum degassing technique alone (η = 89.95% and KLa = 0.0585 s−1). A correlation to predict the KLa was established and the predicted KLa values were in agreement with the experimental values, with deviations generally within 20%. The results indicate that RSR is a promising deaerator thanks to its intensification of gas–liquid contact.
A Review of Crystallization Fouling in Heat Exchangers
Authors: Berce Jure , Zupančič Matevž , Može Matic , Golobič Iztok
Published: August 2021

A vast majority of heat exchangers suffer from unwanted deposition of material on the surface, which severely inhibits their performance and thus marks one of the biggest challenges in heat transfer. Despite numerous scientific investigations, prediction and prevention of fouling remain unresolved issues in process engineering and are responsible for large economic losses and environmental damage. This review article focuses specifically on crystallization fouling, providing a comprehensive overview of the state-of-the-art of fouling in heat exchangers. The fundamentals of the topic are discussed, as the term fouling resistance is introduced along with distinct fouling behaviour, observed in laboratory and industrial environments. Insight into subsequent phases of the fouling process is provided, along with the accompanying microscale events. Furthermore, the effects of fluid composition, temperature, flow velocity, surface condition, nucleate boiling and composite fouling are comprehensively discussed. Fouling modelling is systematically reviewed, from the early work of Kern and Seaton to recently used artificial neural networks and computational fluid dynamics. Finally, the most common fouling mitigation approaches are presented, including design considerations and various on-line strategies, as well as off-line cleaning. According to our review, several topics require further study, such as the initial stage of crystal formation, the effects of ageing, the interplay of two or more fouling mechanisms and the underlying phenomena of several mitigation strategies.
Effect of Raja Yoga Meditation on the Distress and Anxiety Levels of Women with Breast Cancer
Authors: Araújo Raquel Vilanova , Fernandes Ana Fátima Carvalho , Campelo Regina Célia Vilanova , Silva Renan Alves , Nery Inez Sampaio
Published: August 2021

Objective: To evaluate the effect of Raja yoga meditation on the level of distress and anxiety in women with breast cancer. Method: A randomized, controlled, clinical trial was carried out in a specialized center between February and December 2019. The patients in the intervention group (n = 25) participated in four group meditation sessions for one month, and the participants in the control group (n = 25) were exposed to an educational activity for the same period and frequency. Cohen’s d was used to evaluate the effect size. Results: A significant reduction in the level of distress and anxiety was found in the intervention group (p < 0.001). The effect of meditation was average in reducing distress, anxiety, depression, and vital signs. There was also an average effect on the increase in saturation of peripheral oxygen (SPO2). Conclusion: The practice of meditation reduced distress and anxiety more effectively than the usual care practices.
“As by a New Pentecost”: Embodied Prayer in Catholic Charismatic Renewal Following Vatican II
Authors: Blohm Michelle
Published: August 2021

On 25 December 1961, John XXIII convoked the Second Vatican Council with his apostolic constitution Humanae salutis, praying that God would show again the wonders of the newborn Church in Jerusalem “as by a new Pentecost”. Not six years later, in 1967, a group of students at Duquesne University in the United States prayed while on retreat for an infusion of the Holy Spirit that they might also experience the power of Pentecost. They received what they reported to be the gifts of the Holy Spirit, and out of the spiritual experiences of that retreat arose what would become an international movement known as the Catholic Charismatic Renewal. This movement, influenced by Pentecostalism, would develop its own embodied praxis of prayer that seeks a renewed encounter with the power of the Holy Spirit made manifest at Pentecost. This article analyzes the embodied prayer language of the Renewal by drawing from Louis-Marie Chauvet’s distinction between language as mediation (or, symbol) and language as tool (or, sign). It will use Chauvet’s distinction as a hermeneutic to flesh out the relationship between post-Vatican II charismatic prayer practices and their intended purpose of participating in the encounter of Pentecost.
Evaluating Organizational Sustainability: A Multi-Criteria Based-Approach to Sustainable Project Management Indicators
Authors: Stanitsas Marios , Kirytopoulos Konstantinos , Aretoulis Georgios
Published: August 2021

Even though recent studies designate that sustainability should be integrated in project management, this integration remains a complex issue. Hence, there is a need to develop a new approach that would assess the organizational sustainability and reveal to what extent sustainable project management practices are effective. The aim of this research is to propose a Multi-Criteria Decision Analysis-based method to assess the integration of the sustainability philosophy in large-scale organizations via the utilization of sustainable project management-related indicators. By utilising the proposed approach to compare internal organizational structures, the researchers aim to reveal the sustainability integration level within different business units, in order to allow organizations to make decisions toward sustainable practices. The indicators used in the proposed model are related to key aspects of organizations and they measure how the departments’ staff utilize sustainable project management processes in their construction projects. The case study was conducted in a market-leading design, engineering, and project management consultancy organization. Evaluating organizational sustainability can help organizations target their efforts in certain areas (enhancing sustainable outcomes). It can also facilitate data collection, analysis, and future projections.
Modeling and Assessing the Spatial and Vertical Distributions of Potentially Toxic Elements in Soil and How the Concentrations Differ
Authors: Ahado Samuel Kudjo , Nwaogu Chukwudi , Sarkodie Vincent Yaw Oppong , Borůvka Luboš
Published: August 2021

A healthy soil is a healthy ecosystem because humans, animals, plants, and water highly depend upon it. Soil pollution by potentially toxic elements (PTEs) is a serious concern for humankind. The study is aimed at (i) assessing the concentrations of PTEs in soils under a long-term heavily industrialized region for coal and textiles, (ii) modeling and mapping the spatial and vertical distributions of PTEs using a GIS-based ordinary kriging technique, and (iii) identifying the possible sources of these PTEs in the Jizerské Mountains (Jizera Mts.) using a positive matrix factorization (PMF) model. Four hundred and forty-two (442) soil samples were analyzed by applying the aqua regia method. To assess the PTE contents, the level of pollution, and the distribution pattern in soil, the contamination factor (CF) and the pollution load index load (PLI) were applied. ArcGIS-based ordinary kriging interpolation was used for the spatial analysis of PTEs. The results of the analysis revealed that the variation in the coefficient (CV) of PTEs in the organic soil was highest in Cr (96.36%), followed by Cu (54.94%) and Pb (49.40%). On the other hand, the mineral soil had Cu (96.88%), Cr (66.70%), and Pb (64.48%) as the highest in CV. The PTEs in both the organic soil and the mineral soil revealed a high heterogeneous variability. Though the study area lies within the “Black Triangle”, which is a historic industrial site in Central Europe, this result did not show a substantial influence of the contamination of PTEs in the area. In spite of the rate of pollution in this area being very low based on the findings, there may be a need for intermittent assessment of the soil. This helps to curtail any excessive accumulation and escalation in future. The results may serve as baseline information for pollution assessment. It might support policy-developers in sustainable farming and forestry for the health of an ecosystem towards food security, forest safety, as well as animal and human welfare.
Growth and Antioxidant Responses in Iron-Biofortified Lentil under Cadmium Stress
Authors: Bansal Ruchi , Priya Swati , Dikshit Harsh Kumar , Jacob Sherry Rachel , Rao Mahesh , Bana Ram Swaroop , Kumari Jyoti , Tripathi Kuldeep , Kumar Ashok , Kumar Shiv , H. M. Siddique Kadambot
Published: August 2021

Cadmium (Cd) is a hazardous heavy metal, toxic to our ecosystem even at low concentrations. Cd stress negatively affects plant growth and development by triggering oxidative stress. Limited information is available on the role of iron (Fe) in ameliorating Cd stress tolerance in legumes. This study assessed the effect of Cd stress in two lentil (Lens culinaris Medik.) varieties differing in seed Fe concentration (L4717 (Fe-biofortified) and JL3) under controlled conditions. Six biochemical traits, five growth parameters, and Cd uptake were recorded at the seedling stage (21 days after sowing) in the studied genotypes grown under controlled conditions at two levels (100 μM and 200 μM) of cadmium chloride (CdCl2). The studied traits revealed significant genotype, treatment, and genotype × treatment interactions. Cd-induced oxidative damage led to the accumulation of hydrogen peroxide (H2O2) and malondialdehyde in both genotypes. JL3 accumulated 77.1% more H2O2 and 75% more lipid peroxidation products than L4717 at the high Cd level. Antioxidant enzyme activities increased in response to Cd stress, with significant genotype, treatment, and genotype × treatment interactions (p < 0.01). L4717 had remarkably higher catalase (40.5%), peroxidase (43.9%), superoxide dismutase (31.7%), and glutathione reductase (47.3%) activities than JL3 under high Cd conditions. In addition, L4717 sustained better growth in terms of fresh weight and dry weight than JL3 under stress. JL3 exhibited high Cd uptake (14.87 mg g−1 fresh weight) compared to L4717 (7.32 mg g−1 fresh weight). The study concluded that the Fe-biofortified lentil genotype L4717 exhibited Cd tolerance by inciting an efficient antioxidative response to Cd toxicity. Further studies are required to elucidate the possibility of seed Fe content as a surrogacy trait for Cd tolerance.
The Major Role of Eccentricity in the Evolution of Colliding Pulsar-Stellar Winds
Authors: Barkov Maxim V. , Bosch-Ramon Valenti
Published: August 2021

Binary systems that host a massive star and a non-accreting pulsar can be powerful non-thermal emitters. The relativistic pulsar wind and the non-relativistic stellar outflows interact along the orbit, producing ultrarelativistic particles that radiate from radio to gamma rays. To properly characterize the physics of these sources, and better understand their emission and impact on the environment, careful modeling of the outflow interactions, spanning a broad range of spatial and temporal scales, is needed. Full three-dimensional approaches are very computationally expensive, but simpler approximate approaches, while still realistic at the semi-quantitative level, are available. We present here the results of calculations done with a quasi three-dimensional scheme to compute the evolution of the interacting flows in a region spanning in size up to a thousand times the size of the binary. In particular, we analyze for the first time the role of different eccentricities in the large scale evolution of the shocked flows. We find that the higher the eccentricity, the closer the flows behave like a one-side outflow, which becomes rather collimated for eccentricity values ≳0.75. The simulations also unveil that the pulsar and the stellar winds become fully mixed within the grid for low eccentricity systems, presenting a more stochastic behavior at large scales than in the highly eccentric systems.
ISCOs and OSCOs in the Presence of a Positive Cosmological Constant in Massive Gravity
Authors: Rincón Ángel , Panotopoulos Grigoris , Lopes Ilídio , Cruz Norman
Published: August 2021

We study the impact of a non-vanishing (positive) cosmological constant on the innermost and outermost stable circular orbits (ISCOs and OSCOs, respectively) within massive gravity in four dimensions. The gravitational field generated by a point-like object within this theory is known, generalizing the usual Schwarzschild–de Sitter geometry of General Relativity. In the non-relativistic limit, the gravitational potential differs by the one corresponding to the Schwarzschild–de Sitter geometry by a term that is linear in the radial coordinate with some prefactor γ, which is the only free parameter. Starting from the geodesic equations for massive test particles and the corresponding effective potential, we obtain a polynomial of fifth order that allows us to compute the innermost and outermost stable circular orbits. Next, we numerically compute the real and positive roots of the polynomial for several different structures (from the hydrogen atom to stars and globular clusters to galaxies and galaxy clusters) considering three distinct values of the parameter γ, determined using physical considerations, such as galaxy rotation curves and orbital precession. Similarly to the Kottler spacetime, both ISCOs and OSCOs appear. Their astrophysical relevance as well as the comparison with the Kottler spacetime are briefly discussed.
Epicyclic Oscillations around Simpson–Visser Regular Black Holes and Wormholes
Authors: Stuchlík Zdeněk , Vrba Jaroslav
Published: August 2021

We study epicyclic oscillatory motion along circular geodesics of the Simpson–Visser meta-geometry describing in a unique way regular black-bounce black holes and reflection-symmetric wormholes by using a length parameter l. We give the frequencies of the orbital and epicyclic motion in a Keplerian disc with inner edge at the innermost circular geodesic located above the black hole outer horizon or on the our side of the wormhole. We use these frequencies in the epicyclic resonance version of the so-called geodesic models of high-frequency quasi-periodic oscillations (HF QPOs) observed in microquasars and around supermassive black holes in active galactic nuclei to test the ability of this meta-geometry to improve the fitting of HF QPOs observational data from the surrounding of supermassive black holes. We demonstrate that this is really possible for wormholes with sufficiently high length parameter l.
BRST and Superfield Formalism—A Review
Authors: Bonora Loriano , Malik Rudra Prakash
Published: August 2021

This article, which is a review with substantial original material, is meant to offer a comprehensive description of the superfield representations of BRST and anti-BRST algebras and their applications to some field-theoretic topics. After a review of the superfield formalism for gauge theories, we present the same formalism for gerbes and diffeomorphism invariant theories. The application to diffeomorphisms leads, in particular, to a horizontal Riemannian geometry in the superspace. We then illustrate the application to the description of consistent gauge anomalies and Wess–Zumino terms for which the formalism seems to be particularly tailor-made. The next subject covered is the higher spin YM-like theories and their anomalies. Finally, we show that the BRST superfield formalism applies as well to the N=1 super-YM theories formulated in the supersymmetric superspace, for the two formalisms go along with each other very well.
Vacuum Semiclassical Gravity Does Not Leave Space for Safe Singularities
Authors: Arrechea Julio , Barceló Carlos , Boyanov Valentin , Garay Luis J.
Published: August 2021

General relativity predicts its own demise at singularities but also appears to conveniently shield itself from the catastrophic consequences of such singularities, making them safe. For instance, if strong cosmic censorship were ultimately satisfied, spacetime singularities, although present, would not pose any practical problems to predictability. Here, we argue that under semiclassical effects, the situation should be rather different: the potential singularities which could appear in the theory will generically affect predictability, and so one will be forced to analyse whether there is a way to regularise them. For these possible regularisations, the presence and behaviour of matter during gravitational collapse and stabilisation into new structures will play a key role. First, we show that the static semiclassical counterparts to the Schwarzschild and Reissner–Nordström geometries have singularities which are no longer hidden behind horizons. Then, we argue that in dynamical scenarios of formation and evaporation of black holes, we are left with only three possible outcomes which could avoid singularities and eventual predictability issues. We briefly analyse the viability of each one of them within semiclassical gravity and discuss the expected characteristic timescales of their evolution.
Propylthiouracil-Induced Antineutrophil Cytoplasmic Antibody-Associated Vasculitis after COVID-19 Vaccination
Authors: Okuda Saki , Hirooka Yasuaki , Sugiyama Masafumi
Published: August 2021

We report the case of a patient who developed antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) after receiving the coronavirus disease 2019 (COVID-19) vaccine BNT162b (Pfizer–BioNTech). A 37-year-old Japanese woman had been taking propylthiouracil for Graves’ disease. She had erythema on her forearm on the 12th day after receiving the first dose of the vaccine, fever on the 13th day, and redness and swelling of her left auricle on the 25th day. Her serum myeloperoxidase-ANCA and proteinase 3-ANCA levels, which were negative before the Graves’ disease treatment, were elevated. She had unilateral auricular symptoms but no other typical relapsing polychondritis findings. She was diagnosed with propylthiouracil-induced AAV. She was treated with oral glucocorticoids, and her symptoms improved. Propylthiouracil is considered to be the main cause of the onset of AAV in this case, but it cannot be ruled out that BNT162b may have had some effect on the onset of the disease. Although the development of propylthiouracil-induced AAV in this case may have been incidental and unrelated to the vaccination, this report provides important data for evaluating the safety of the vaccine.
Influenza Vaccination in Psoriatic Patients—Epidemiology and Patient Perceptions: A German Multicenter Study (Vac-Pso)
Authors: Kromer Christian , Wellmann Phoebe , Siemer Ralf , Klein Selina , Mohr Johannes , Pinter Andreas , Wilsmann-Theis Dagmar , Mössner Rotraut
Published: August 2021

The risk of developing severe complications from an influenza virus infection is increased in patients with chronic inflammatory diseases such as psoriasis (PsO) and atopic dermatitis (AD). However, low influenza vaccination rates have been reported. The aim of this study was to determine vaccination rates in PsO compared to AD patients and explore patient perceptions of vaccination. A multicenter cross-sectional study was performed in 327 and 98 adult patients with PsO and AD, respectively. Data on vaccination, patient and disease characteristics, comorbidity, and patient perceptions was collected with a questionnaire. Medical records and vaccination certificates were reviewed. A total of 49.8% of PsO and 32.7% of AD patients were vaccinated at some point, while in season 2018/2019, 30.9% and 13.3% received an influenza vaccination, respectively. There were 96.6% and 77.6% of PsO and AD patients who had an indication for influenza vaccination due to age, immunosuppressive therapy, comorbidity, occupation, and/or pregnancy. Multivariate regression analysis revealed higher age (p < 0.001) and a history of bronchitis (p = 0.023) as significant predictors of influenza vaccination in PsO patients. Considering that most patients had an indication for influenza vaccination, the rate of vaccinated patients was inadequately low.
Protective Efficacy of Novel Oral Biofilm Vaccines against Lactococcus garvieae Infection in Mullet, Mugil cephalus
Authors: Su Feng-Jie , Chen Meei-Mei
Published: August 2021

Lactococcus garvieae (L. garvieae) is an important pathogen that causes enormous economic losses in both marine and freshwater aquaculture. At present, antibiotics are the only option for farmers to reduce the losses caused by L. garvieae. However, the usage of antibiotics leads to environmental pollution and the production of drug-resistant strains of bacteria. Therefore, vaccination is preferred as an alternative method to prevent infectious diseases. In this study, we describe an effective approach to the production of an oral biofilm vaccine, using bacteria grown on chitosan particles to form biofilms, and thus providing an inactive pathogen that enhances the immune response in fish. We observed the formation of a biofilm on chitosan particles and administered the novel oral biofilm vaccine to fish. We analyzed the immune responses, including antibody production, phagocytic ability, albumin/globulin ratio and immune-related genes, of vaccinated and control groups of black mullet. Our results show that the phagocytic ability of the biofilm vaccine group was 84%, which is significantly higher than that of the control group, and the antibody production in this group was significantly higher compared with the other group. The mRNA expression levels of immune-related genes (TLR2, IL-1β, TNF-α) were significantly upregulated in the spleen after vaccination. In challenge experiments, the relative percent survival (RPS) was 77% in the biofilm vaccine group, 18% in the whole-cell vaccine group, and 0% in the chitosan particle group at 32 days post-vaccination. In addition, we also found that the relative percent survival (RPS) at 1 day post-vaccination was 74% in the biofilm vaccine group, 42% in the whole-cell vaccine group, and 26% in the chitosan particle group. In both long-term and short-term challenge experiments, the viability of the biofilm vaccine group was significantly higher than that of the whole-cell, chitosan particle and PBS groups. We conclude that based on its protective effect, the L. garvieae biofilm vaccine is better than the whole-cell vaccine when challenged several weeks after vaccination. In addition, the biofilm vaccine also has a greater protective effect than the whole-cell vaccine when challenged immediately after vaccination. Therefore, the biofilm vaccine might represent a novel method for the prevention and treatment of L. garvieae infection.
The role of the general practitioner in the system of providing cardiac care to the population
Authors: Grigoriev Konstantin Robertovich
Published: August 2021

Diseases of the cardiovascular system continue to occupy a leading position in the structure of the general morbidity of the population, and mortality from this pathology ranks first in almost all countries of the world. In most cases, a general practitioner is the first person to whom patients turn for help, so s/he must have a fairly extensive knowledge base and be able to navigate a complex variety of nosological forms and syndromes. In many cases, a correct diagnosis and a correctly prescribed treatment can significantly improve the quality of life of people suffering from pathology of the cardiovascular system. Under the condition of provision of the necessary material and technical equipment, the professional training of a general practitioner allows this specialist to consider a wide range of outpatient cardiac problems, provide preventive and therapeutic assistance to the assigned contingent, and be responsible for the health of the population served.