bluuebunny/crossref_metadata_2025 huggingface dataset:
Computational Characterization of Membrane Proteins as Anticancer Targets: Current Challenges and Opportunities
Authors: Gorostiola González Marina , Rakers Pepijn R. J. , Jespers Willem , IJzerman Adriaan P. , Heitman Laura H. , van Westen Gerard J. P.
Published: March 2024

Cancer remains a leading cause of mortality worldwide and calls for novel therapeutic targets. Membrane proteins are key players in various cancer types but present unique challenges compared to soluble proteins. The advent of computational drug discovery tools offers a promising approach to address these challenges, allowing for the prioritization of “wet-lab” experiments. In this review, we explore the applications of computational approaches in membrane protein oncological characterization, particularly focusing on three prominent membrane protein families: receptor tyrosine kinases (RTKs), G protein-coupled receptors (GPCRs), and solute carrier proteins (SLCs). We chose these families due to their varying levels of understanding and research data availability, which leads to distinct challenges and opportunities for computational analysis. We discuss the utilization of multi-omics data, machine learning, and structure-based methods to investigate aberrant protein functionalities associated with cancer progression within each family. Moreover, we highlight the importance of considering the broader cellular context and, in particular, cross-talk between proteins. Despite existing challenges, computational tools hold promise in dissecting membrane protein dysregulation in cancer. With advancing computational capabilities and data resources, these tools are poised to play a pivotal role in identifying and prioritizing membrane proteins as personalized anticancer targets.
[Cu(NN1)2]ClO4, a Copper (I) Complex as an Antimicrobial Agent for the Treatment of Piscirickettsiosis in Atlantic Salmon
Authors: Parra Mick , Aldabaldetrecu Maialen , Arce Pablo , Soto-Aguilera Sarita , Vargas Rodrigo , Guerrero Juan , Tello Mario , Modak Brenda
Published: March 2024

Piscirickettsia salmonis is the pathogen that most affects the salmon industry in Chile. Large quantities of antibiotics have been used to control it. In search of alternatives, we have developed [Cu(NN1)2]ClO4 where NN1 = 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one. The antibacterial capacity of [Cu(NN1)2]ClO4 was determined. Subsequently, the effect of the administration of [Cu(NN1)2]ClO4 on the growth of S. salar, modulation of the immune system and the intestinal microbiota was studied. Finally, the ability to protect against a challenge with P. salmonis was evaluated. The results obtained showed that the compound has an MIC between 15 and 33.9 μg/mL in four isolates. On the other hand, the compound did not affect the growth of the fish; however, an increase in the transcript levels of IFN-γ, IL-12, IL-1β, CD4, lysozyme and perforin was observed in fish treated with 40 μg/g of fish. Furthermore, modulation of the intestinal microbiota was observed, increasing the genera of beneficial bacteria such as Lactobacillus and Bacillus as well as potential pathogens such as Vibrio and Piscirickettsia. Finally, the treatment increased survival in fish challenged with P. salmonis by more than 60%. These results demonstrate that the compound is capable of protecting fish against P. salmonis, probably by modulating the immune system and the composition of the intestinal microbiota.
Bioengineering Skin Substitutes for Wound Management—Perspectives and Challenges
Authors: Kondej Karolina , Zawrzykraj Małgorzata , Czerwiec Katarzyna , Deptuła Milena , Tymińska Agata , Pikuła Michał
Published: March 2024

Non-healing wounds and skin losses constitute significant challenges for modern medicine and pharmacology. Conventional methods of wound treatment are effective in basic healthcare; however, they are insufficient in managing chronic wound and large skin defects, so novel, alternative methods of therapy are sought. Among the potentially innovative procedures, the use of skin substitutes may be a promising therapeutic method. Skin substitutes are a heterogeneous group of materials that are used to heal and close wounds and temporarily or permanently fulfill the functions of the skin. Classification can be based on the structure or type (biological and synthetic). Simple constructs (class I) have been widely researched over the years, and can be used in burns and ulcers. More complex substitutes (class II and III) are still studied, but these may be utilized in patients with deep skin defects. In addition, 3D bioprinting is a rapidly developing method used to create advanced skin constructs and their appendages. The aforementioned therapies represent an opportunity for treating patients with diabetic foot ulcers or deep skin burns. Despite these significant developments, further clinical trials are needed to allow the use skin substitutes in the personalized treatment of chronic wounds.
Differences in Neuropathology between Nitroglycerin-Induced Mouse Models of Episodic and Chronic Migraine
Authors: Park Songyi , Jung Harry , Han Sang-Won , Lee Sang-Hwa , Sohn Jong-Hee
Published: March 2024

Multiple animal models of migraine have been used to develop new therapies. Understanding the transition from episodic (EM) to chronic migraine (CM) is crucial. We established models mimicking EM and CM pain and assessed neuropathological differences. EM and CM models were induced with single NTG or multiple injections over 9 days. Mechanical hypersensitivity was assessed. Immunofluorescence utilized c-Fos, NeuN, and Iba1. Proinflammatory and anti-inflammatory markers were analyzed. Neuropeptides (CGRP, VIP, PACAP, and substance P) were assessed. Mechanical thresholds were similar. Notable neuropathological distinctions were observed in Sp5C and ACC. ACC showed increased c-Fos and NeuN expression in CM (p < 0.001) and unchanged in EM. Sp5C had higher c-Fos and NeuN expression in EM (p < 0.001). Iba1 was upregulated in Sp5C of EM and ACC of CM (p < 0.001). Proinflammatory markers were strongly expressed in Sp5C of EM and ACC of CM. CGRP expression was elevated in both regions and was higher in CM. VIP exhibited higher levels in the Sp5C of EM and ACC of CM, whereas PACAP and substance P were expressed in the Sp5C in both models. Despite similar thresholds, distinctive neuropathological differences in Sp5C and ACC between EM and CM models suggest a role in the EM to CM transformation.
Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis in Panax quinquefolius
Authors: Song Guimei , Yan Yan , Guo Chun , Chen Jiankang , Wang Yumeng , Wang Yingping , Zhang Jiaxin , Gao Chang , Lian Junmei , Piao Xiangmin , Di Peng
Published: March 2024

Panax quinquefolius L. is an important medicinal plant, and flavonoids are among its main secondary metabolites. The R2R3-MYB transcription factor plays an irreplaceable role in plant growth, development, and secondary metabolism. In our study, we identified 159 R2R3-MYBs and analyzed their physical and chemical properties in P. quinquefolius. The protein length of 159 PqMYBs varied from 107 to 1050 amino acids. The molecular weight ranged from 12.21 to 116.44 kDa. The isoelectric point was between 4.57 and 10.34. We constructed a phylogenetic tree of P. quinquefolius and Arabidopsis thaliana R2R3-MYB family members, and PqMYB members were divided into 33 subgroups. Transcriptome data analysis showed that the expression patterns of PqMYBs in root, leaf, and flower were significantly different. Following the MeJA treatment of seedlings, five candidate PqMYB genes demonstrated a response. A correlation analysis of PqMYBs and candidate flavonoid pathway genes showed that PqMYB2, PqMYB46, and PqMYB72 had correlation coefficients that were higher than 0.8 with PqCHS, PqANS4, and PqCCoAMT10, respectively. Furthermore, a transient expression assay confirmed that the three PqMYBs were localized in the nucleus. We speculated that these three PqMYBs were related to flavonoid biosynthesis in P. quinquefolius. These results provided a theoretical basis and a new perspective for further understanding the R2R3-MYB gene family and the biosynthesis mechanism of secondary metabolites in P. quinquefolius.
Ventilator Weaning in Prolonged Mechanical Ventilation—A Narrative Review
Authors: Dolinay Tamás , Hsu Lillian , Maller Abigail , Walsh Brandon Corbett , Szűcs Attila , Jerng Jih-Shuin , Jun Dale
Published: March 2024

Patients requiring mechanical ventilation (MV) beyond 21 days, usually referred to as prolonged MV, represent a unique group with significant medical needs and a generally poor prognosis. Research suggests that approximately 10% of all MV patients will need prolonged ventilatory care, and that number will continue to rise. Although we have extensive knowledge of MV in the acute care setting, less is known about care in the post-ICU setting. More than 50% of patients who were deemed unweanable in the ICU will be liberated from MV in the post-acute setting. Prolonged MV also presents a challenge in care for medically complex, elderly, socioeconomically disadvantaged and marginalized individuals, usually at the end of their life. Patients and their families often rely on ventilator weaning facilities and skilled nursing homes for the continuation of care, but home ventilation is becoming more common. The focus of this review is to discuss recent advances in the weaning strategies in prolonged MV, present their outcomes and provide insight into the complexity of care.
Safety and Efficacy of Zero-Profile Polyetheretherketone (PEEK) Cages Filled with Biphasic Calcium Phosphate (BCP) in Anterior Cervical Discectomy and Fusion (ACDF): A Case Series
Authors: Battistelli Marco , Mazzucchi Edoardo , Muselli Mario , Galieri Gianluca , Polli Filippo Maria , Pignotti Fabrizio , Olivi Alessandro , Sabatino Giovanni , La Rocca Giuseppe
Published: March 2024

Background: In the evolving landscape of anterior cervical discectomy and fusion (ACDF), the integration of biomechanical advancements and proper fusion-enhancing materials is crucial for optimizing patient outcomes. This case series evaluates the efficacy and clinical implications of employing zero-profile polyetheretherketone (PEEK) cages filled with biphasic calcium phosphate (BCP) in ACDF procedures, focusing on fusion and subsidence rates alongside patient disability, residual pain, and quality of life. Methods: This case series comprises 76 consecutive patients, with a median follow-up of 581 days. The Bridwell classification system was used for assessing fusion rates while subsidence occurrence was recorded, correlating these radiographic outcomes with clinical implications. Results: The results demonstrated a satisfactory fusion rate (76.4% for grades I and II). The subsidence rate was low (6.74% of segments). Significant clinical improvements were observed in pain, disability, and quality-of-life metrics, aligning with the minimum clinically important difference thresholds; however, subgroup analyses demonstrated that subsidence or pseudoarthrosis group improvement of PROMs was not statistically significant with respect to baseline. ANOVA analyses documented that subsidence has a significant weight over final follow-up pain and disability outcomes. No dysphagia cases were reported. Conclusions: These findings underscore the efficacy of zero-profile PEEK cages filled with BCP in ACDF, highlighting their potential to improve patient outcomes while minimizing complications. Pseudoarthrosis and subsidence have major implications over long-term PROMs. The study reinforces the importance of selecting appropriate surgical materials to enhance the success of ACDF procedures.
Special Considerations in Pediatric Endoscopic Skull Base Surgery
Authors: Valencia-Sanchez Bastien A. , Kim Jeeho D. , Zhou Sheng , Chen Sonja , Levy Michael L. , Roxbury Christopher , Patel Vijay A. , Polster Sean P.
Published: March 2024

Originally pioneered in adults, endoscopic endonasal approaches for skull base pathology are being increasingly applied as a minimally invasive alternative for young children. Intrinsic anatomic differences between these patient populations have sparked discussions on the feasibility, safety, and efficacy of these techniques in pediatric patients. This work aims to serve as a primer for clinicians engaged in the rapidly evolving field of pediatric endoscopic skull base surgery. A succinct overview of relevant embryology, sinonasal anatomy, and diagnostic workup is presented to emphasize key differences and unique technical considerations. Additional discussions regarding select skull base lesions, reconstructive paradigms, potential surgical complications, and postoperative care are also highlighted in the setting of multidisciplinary teams.
Examining the Existence of Cognitive Thresholds in Highly Quantitative College Courses
Authors: Zhou You , Kuncel Nathan R. , Sackett Paul R.
Published: March 2024

While the dominant finding indicates a monotonic relationship between cognitive ability and academic performance, some researchers have suggested the existence of cognitive thresholds for challenging coursework, such that a certain level of cognitive ability is required for reaching a satisfactory level of academic achievement. Given the significance of finding a threshold for understanding the relationship between cognitive ability and academic performance, and the limited studies on the topic, it is worth further investigating the possibility of cognitive thresholds. Using a multi-institutional dataset and the necessary condition analysis (NCA), we attempted to replicate previous findings of cognitive thresholds on the major GPA of mathematics and physics-majored students, as well as the course grade of organic chemistry, to examine whether high SAT math scores constitute a necessary condition for obtaining satisfactory grades in these courses. The results from the two studies do not indicate an absolute cognitive threshold point below which students are doomed to fail regardless of the amount of effort they devote into learning. However, we did find that the chance of students with a low level of quantitative ability to succeed in highly quantitative courses is very small, which qualifies for the virtually necessary condition.
Characterizing the Palm Pathogenic Thielaviopsis Species from Florida
Authors: Ayika Marie-Gabrielle , Rosano Avril , Valiente Jacqueline , Chakrabarti Seemanti , Rollins Jeffrey A. , Dhillon Braham
Published: March 2024

Thielaviopsis paradoxa sensu lato is a soilborne fungal pathogen that causes Thielaviopsis trunk rot and heart rot in palms. The loss of structural integrity resulting from trunk rot can cause the palm trunk to collapse suddenly and poses a serious threat to life and property. Even though rudimentary knowledge about the Thielaviopsis infection process in palms is available, nothing is known about the T. paradoxa species complex in the US. The aim of this study was to characterize T. paradoxa s. lat. isolates collected from diseased palms grown in Florida. Multi-locus phylogeny using three genes, ITS, β-tubulin, and tef1-α, revealed that the isolates separate into two distinct clades with high bootstrap support. The majority of the isolates clustered with the species T. ethacetica, while two isolates formed a separate clade, distinct from T. musarum, and might represent an undescribed Thielaviopsis species. One representative isolate from each clade, when grown on three distinct media and at four different temperatures, showed differences in gross colony morphology, as well as growth rates. The T. ethacetica isolate TP5448 and the Thielaviopsis sp. isolate PLM300 grew better at opposite ends of the temperature spectrum tested in this study, i.e., 35 °C and 10 °C, respectively. In pathogenicity assays on whole plants, the T. ethacetica isolate proved to be more aggressive than Thielaviopsis sp. isolate PLM300, as it produced larger lesions when inoculated on wounded leaflets. An unequal distribution was observed for the mating-type locus of T. ethacetica, as 12 isolates carried the MAT1-1-1 allele, while the status for four isolates remained undefined. Variation in mycelial growth in response to different fungicides was also observed between the two clades. These results demonstrate the existence of two Thielaviopsis clades that can infect palms in Florida and underscore the need for targeted sampling to help uncover the diversity of Thielaviopsis species across palm-growing regions in the US.
Integrated Management of the Cotton Charcoal Rot Disease Using Biological Agents and Chemical Pesticides
Authors: Degani Ofir , Chen Assaf , Dimant Elhanan , Gordani Asaf , Malul Tamir , Rabinovitz Onn
Published: March 2024

Charcoal rot disease (CRD), caused by the phytopathogenic fungus Macrophomina phaseolina, is a significant threat to cotton production in Israel and worldwide. The pathogen secretes toxins and degrading enzymes that disrupt the water and nutrient uptake, leading to death at the late stages of growth. While many control strategies were tested over the years to reduce CRD impact, reaching that goal remains a significant challenge. The current study aimed to establish, improve, and deepen our understanding of a new approach combining biological agents and chemical pesticides. Such intervention relies on reducing fungicides while providing stability and a head start to eco-friendly bio-protective Trichoderma species. The research design included sprouts in a growth room and commercial field plants receiving the same treatments. Under a controlled environment, comparing the bio-based coating treatments with their corresponding chemical coating partners resulted in similar outcomes in most measures. At 52 days, these practices gained up to 38% and 45% higher root and shoot weight and up to 78% decreased pathogen root infection (tracked by Real-Time PCR), compared to non-infected control plants. Yet, in the shoot weight assessment (day 29 post-sowing), the treatment with only biological seed coating outperformed (p < 0.05) all other biological-based treatments and all Azoxystrobin-based irrigation treatments. In contrast, adverse effects are observed in the chemical seed coating group, particularly in above ground plant parts, which are attributable to the addition of Azoxystrobin irrigation. In the field, the biological treatments had the same impact as the chemical intervention, increasing the cotton plants’ yield (up to 17%), improving the health (up to 27%) and reducing M. phaseolina DNA in the roots (up to 37%). When considering all treatments within each approach, a significant benefit to plant health was observed with the bio-chemo integrated management compared to using only chemical interventions. Specific integrated treatments have shown potential in reducing CRD symptoms, such as applying bio-coating and sprinkling Azoxystrobin during sowing. Aerial remote sensing based on high-resolution visible-channel (RGB), green–red vegetation index (GRVI), and thermal imaging supported the above findings and proved its value for studying CRD control management. This research validates the combined biological and chemical intervention potential to shield cotton crops from CRD.
Aquatic Fate and Ecotoxicology Effect of ZnS:Mn Quantum Dots on Chlorella vulgaris in Fresh Water
Authors: Deng Bingbing , Maaloul Rania , Nowak Sophie , Sivry Yann , Yéprémian Claude , Ammar Souad , Mammeri Fayna , Brayner Roberta
Published: March 2024

With the increasing integration of nanomaterials into daily life, the potential ecotoxicological impacts of nanoparticles (NPs) have attracted increased attention from the scientific community. This study assessed the ecotoxicity of ZnS quantum dots (QDs) doped with varying molar concentrations of Mn2+ on Chlorella vulgaris. The ZnS:Mn QDs were synthesized using the polyol method. The size of the ZnS:Mn QDs ranged from approximately 1.1 nm to 2 nm, while the aggregation size in Seine River water was 341 nm at pH 6 and 8. The presence of ZnS:Mn (10%) NPs exhibited profound toxicity to Chlorella vulgaris, with immediate reductions in viability (survival cells) from 71%, 60% to 51%, 52% in BG11 and Seine River water, respectively, at a concentration of 100 mg L−1 of ZnS:Mn (10%) NPs. Additionally, the ATP content in Chlorella vulgaris significantly decreased in Seine River water (by 20%) after 3 h of exposure to ZnS:Mn (10%) NPs. Concurrently, SOD activity significantly increased in Seine River water, indicating that the ZnS:Mn (10%) NPs induced ROS production and triggered an oxidative stress response in microalgae cells.
Investigating ABO Blood Groups and Secretor Status in Relation to SARS-CoV-2 Infection and COVID-19 Severity
Authors: Ferous Stefanos , Siafakas Nikolaos , Boufidou Fotini , Patrinos George P. , Tsakris Athanasios , Anastassopoulou Cleo
Published: March 2024

The ABO blood groups, Lewis antigens, and secretor systems are important components of transfusion medicine. These interconnected systems have been also shown to be associated with differing susceptibility to bacterial and viral infections, likely as the result of selection over the course of evolution and the constant tug of war between humans and infectious microbes. This comprehensive narrative review aimed to explore the literature and to present the current state of knowledge on reported associations of the ABO, Lewis, and secretor blood groups with SARS-CoV-2 infection and COVID-19 severity. Our main finding was that the A blood group may be associated with increased susceptibility to SARS-CoV-2 infection, and possibly also with increased disease severity and overall mortality. The proposed pathophysiological pathways explaining this potential association include antibody-mediated mechanisms and increased thrombotic risk amongst blood group A individuals, in addition to altered inflammatory cytokine expression profiles. Preliminary evidence does not support the association between ABO blood groups and COVID-19 vaccine response, or the risk of developing long COVID. Even though the emergency state of the pandemic is over, further research is needed especially in this area since tens of millions of people worldwide suffer from lingering COVID-19 symptoms.
The Effect of Indian Contact and Glaswegian Contact on the Phonetic Backward Transfer of Glaswegian English (L2) on Hindi and Indian English (L1)
Authors: Shaktawat Divyanshi
Published: March 2024

This study examined phonetic backward transfer in ‘Glaswasians’, the ethnolinguistic minority of first-generation bilingual immigrant Indians in Glasgow (Scotland), who present a situation of contact between their native languages of Hindi and Indian English (L1s) and the dominant host language and dialect, Glaswegian English (L2). This was examined in relation to the Revised Speech Learning Model (SLM-r) and Speech Accommodation Framework. These predict that the migrants’ L1 sound categories can either shift to become more Glaswegian-like (‘assimilation’ or ‘convergence’) or exaggeratedly Indian-like (‘dissimilation’ or ‘divergence’) or remain unchanged. The effect of Indian and Glaswegian Contact on transfer was also investigated. Two control groups (Indians and Glaswegians) and the experimental group (Glaswasians) were recorded reading English and Hindi sentences containing multiple phones which were examined for multiple phonetic features (/t/—VOT, /l/—F2-F1 difference, /b d g/—Relative Burst Intensity). In both languages, Glaswasian /t/ and /g/ became more Glaswegian-like (assimilation), whereas F2-F1 difference in /l/ became exaggeratedly Indian-like (dissimilation). Higher Indian Contact was associated with more native-like values in /t/ and /l/ in Hindi but had no influence on /g/. Higher Glaswegian Contact was related to increased assimilation of /g/ in English but had no effect on /l/ and /t/.
Who’s Really Got the Right Moves? Analyzing Recommendations for Writing American Judicial Opinions
Authors: Lavissière Mary C. , Bonnard Warren
Published: March 2024

There is little linguistic research on the structure of judicial opinions from a discourse analysis perspective. There are, however, many professional resources about writing judicial opinions. This paper contributes to genre theory and linguistics of languages for specific purposes by proposing a role for professional writing advice. We also construct a typology of macrostructures proposed by professionals and compare them to the move structure of authentic judicial opinions. Our results show that, in terms of large discourse units, professional resources and move analysis seem to converge. Professional resources, however, do not describe the variation that may be observed in authentic documents. In this way, corpora of professional advice may contribute to a deeper understanding of how a discourse community represents its own genres.
Path-Following Sliding Mode Controller for an Electric Vehicle Considering Actuator Dynamics
Authors: Torres-Romero Luis Arturo , Ruiz-Cruz Riemann , González-Jiménez Luis Enrique
Published: March 2024

This study introduced a novel path-following controller tailored to electric vehicles equipped with a steer-by-wire system, i.e., the steering angle of the vehicle was defined by an electrical actuator. The control objective was to force the proper steering angle of the vehicle, which permits following a desired path. The system presupposed that an external algorithm that utilized sensor data provided the lateral movement references while maintaining a steady longitudinal velocity for the vehicle. The proposed control scheme was based on a robust sliding mode steering controller to manage the vehicle’s lateral movement. Furthermore, a brushless DC (BLDC) motor was considered as the steering actuator, which was controlled by a field-oriented controller (FOC), which was based on four internal proportional–integral (PI) control loops for precise steering actuation. To assess the performance of the proposed control scheme, numerical simulations were obtained, which demonstrated its effectiveness in achieving the control objective.
Monitoring of Stator Winding Insulation Degradation through Estimation of Stator Winding Temperature and Leakage Current
Authors: Szamel Laszlo , Oloo Jackson
Published: March 2024

Switched Reluctance Motors (SRMs), Permanent Magnet Synchronous Motors (PMSMs), and induction motors may experience failures due to insulation-related breakdowns. The SRM rotor is of a non-salient nature and made of solid steel material. There are no windings on the rotor. However, the stator is composed of windings that are intricately insulated from each other using materials such as enamel wire, polymer films, mica tapes, epoxy resin, varnishes, or insulating tapes. The dielectric strength of the insulation may fail over time due to several environmental factors and processes. Dielectric breakdown of the winding insulation can be caused by rapid switching of the winding current, the presence of contaminants, and thermal aging. For reliable and efficient operation of the SRMs and other electrical machines, it is necessary to take into account the physics of the winding insulation and perform appropriate diagnostics and estimations that can monitor the integrity of the insulation. This article presents the estimation problem using a Genetic Algorithm (GA)-optimized Random Forest Regressor. Empirical properties and measurable quantities in the historical data are utilized to derive temperature and leakage current estimation. The developed model is then combined with a moving average function to increase the accuracy of prediction of the stator winding temperature and leakage current. The performance of the model is compared with that of the Feedforward Neural Network and Long Short-Term Memory over the same winding temperature and leakage current historical data. The performance metrics are based on computation of the Mean Square Error and Mean Absolute Error.
Restricted Singular Value Decomposition for a Tensor Triplet under T-Product and Its Applications
Authors: Zhang Chong-Quan , Wang Qing-Wen , Wang Xiang-Xiang , He Zhuo-Heng
Published: March 2024

We investigate and discuss in detail the structure of the restricted singular value decomposition for a tensor triplet under t-product (T-RSVD). The algorithm is provided with a numerical example illustrating the main result. For applications, we consider color image watermarking processing with T-RSVD.
Erasure Recovery Matrices for Data Erasures and Rearrangements
Authors: He Miao , Wu Changtian , Leng Jinsong
Published: March 2024

When studying signal reconstruction, the frames are often selected in advance as encoding tools. However, in practical applications, this encoding frame may be subject to attacks by intermediaries and generate errors. To solve this problem, in this paper, the erasure recovery matrices for data erasures and rearrangements are analyzed. Unlike the previous research, first of all, we introduce a kind of frame and its erasure recovery matrix M so that MI,Λ=Im×m, where Im×m is a unit matrix. In this case, we do not need to invert the matrix of the frame operator and the erasure recovery matrix, and this greatly simplifies reconstruction problems and calculations. Then three different construction algorithms of the above erasure recovery matrix M and the frame are proposed, and each of them has advantages. Furthermore, some restrictions on M so that the constructed frame and erasure recovery matrix M can recover coefficients from rearrangements are imposed. We prove that in some cases, the above M and frame can recover coefficients stably from m rearrangements.
Lemneolemnanes A–D, Four Uncommon Sesquiterpenoids from the Soft Coral Lemnalia sp.
Authors: Zong Yuan , Jin Tian-Yun , Yang Jun-Jie , Wang Kun-Ya , Shi Xing , Zhang Yue , Li Ping-Lin
Published: March 2024

Four undescribed sesquiterpenoids, lemneolemnanes A–D (1–4), have been isolated from the marine soft coral Lemnalia sp. The absolute configurations of the stereogenic carbons of 1–4 were determined by single-crystal X-ray crystallographic analysis. Compounds 1 and 2 are epimers at C-3 and have an unusual skeleton with a formyl group on C-6. Compound 3 possesses an uncommonly rearranged carbon skeleton, while 4 has a 6/5/5 tricyclic system. Compound 1 showed significant anti-Alzheimer’s disease (AD) activity in a humanized Caenorhabditis elegans AD pathological model.
Atrial Fibrillation Burden: Impact on Stroke Risk and Beyond
Authors: AlTurki Ahmed , Essebag Vidal
Published: March 2024

Atrial fibrillation (AF) is an important independent risk factor for stroke. Current guidelines handle AF as a binary entity with risk driven by the presence of clinical risk factors, which guides the decision to treat with an oral anticoagulant. Recent studies in the literature suggest a dose–response relationship between AF burden and stroke risk, in both clinical AF and subclinical atrial fibrillation (SCAF), which differs from current guidance to disregard burden and utilize clinical risk scores alone. Within clinical classification and at the same risk levels in various scores, the risk of stroke increases with AF burden. This opens the possibility of incorporating burden into risk profiles, which has already shown promise. Long-term rhythm monitoring is needed to elucidate SCAF in patients with stroke. Recent data from randomized trials are controversial regarding whether there is an independent risk from AF episodes with a duration of less than 24 h, including the duration of SCAF greater than six minutes but less than 24 h.
Detection and Imaging of Corrosion Defects in Steel Structures Based on Ultrasonic Digital Image Processing
Authors: Chi Dazhao , Xu Zhixian , Liu Haichun
Published: March 2024

Corrosion is one of the critical factors leading to the failure of steel structures. Ultrasonic C-scans are widely used to identify corrosion damage. Limited by the range of C-scans, multiple C-scans are usually required to cover the whole component. Thus, stitching multiple C-scans into a panoramic image of the area under detection is necessary for interpreting non-destructive testing (NDT) data. In this paper, an image mosaic method for ultrasonic C-scan based on scale invariant feature transform (SIFT) is proposed. Firstly, to improve the success rate of registration, the difference in the probe starting position in two scans is used to filter the matching pairs of feature points obtained by SIFT. Secondly, dynamic programming methods are used to search for the optimal seam path. Finally, the pixels in the overlapping area are fused by fade-in and fade-out fusion along the seam line. The improved method has a higher success rate of registration and lower image distortion than the conventional method in the mosaic of ultrasonic C-scan images. Experimental results show that the proposed method can stitch multiple C-scan images of a testing block containing artificial defects into a panorama image effectively.
Metabolic Profiling of SH-SY5Y and Neuro2A Cells in Relation to Fetal Calf Serum (FCS) Concentration in Culture Media
Authors: Kronenberger Lys , Mett Janine , Hoppstädter Jessica , Müller Uli
Published: March 2024

The neuroblastoma cell lines SH-SY5Y and Neuro2A are commonly utilized models in neurobiological research. DMEM supplemented with different nutrients and 5–10% Fetal Calf Serum (FCS) is typically used for culturing these cell lines. During special treatments, a reduced FCS content is often deployed to reduce cellular proliferation or the content of bioactive compounds. The impact of the reduction of FCS in culture media on the metabolic profile of SH-SY5Y and Neuro2A cells is currently unknown. Using an Amplex Red Assay, this study showed that the consumption of L-glutamine decreased after FCS reduction. Glucose and pyruvate consumption increased in both cell lines after the reduction of FCS. Thus, lactate production also increased with reduced FCS concentration. The reduction of FCS in the cell culture medium resulted in a reduced aerobic ATP production for SH-SY5Y cells and a complete shut down of aerobic ATP production for Neuro2A cells, measured using the Seahorse XF Real-Time ATP Rate Assay. Utilizing the Seahorse XF Glutamine Oxidation Stress Test, Neuro2A cells showed an increased utilization of L-glutamine oxidation after reduction of FCS. These results indicate that changes in FCS concentration in culture media have an impact on the different energy production strategies of SH-SY5Y and Neuro2A cells which must be considered when planning special treatments.
Characterization of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Animal Farms in Hunan Province, China
Authors: Xiao Ning , Li Yujuan , Lin Hongguang , Yang Jie , Xiao Gang , Jiang Zonghan , Zhang Yunqiang , Chen Wenxin , Zhou Pengcheng , Sun Zhiliang , Li Jiyun
Published: March 2024

Multi-drug resistance of bacteria producing extended-spectrum β-lactamase (ESBL) is a public health challenge. Thus, this study aimed to investigate the antimicrobial susceptibility of ESBL-producing Escherichia coli (ESBL-EC) in Hunan Province, China. A total of 1366 fecal samples were collected from pig, chicken, and cattle farms over a six-year period, which were assessed using strain isolation, 16S rRNA identification, polymerase chain reaction, drug sensitivity testing, whole-genome sequencing, and bioinformatics analysis. The results showed an overall prevalence of 6.66% for ESBL-EC strains, with ESBL positivity extents for pigs, chickens, and cattle isolates at 6.77%, 6.54%, and 12.5%, respectively. Most ESBL-EC isolates were resistant to cefotaxime, tetracycline, and trimethoprim-sulfamethoxazole; however, all the isolates were susceptible to meropenem, with relatively low resistance to amikacin and tigecycline. Various multi-locus sequence types with different origins and similar affinities were identified, with ST155 (n = 16) being the most common subtype. Several types of resistance genes were identified among the 91 positive strains, with beta-lactamase blaCTX-M-55 being the most common ESBL genotype. IncFIB was the predominant plasmid type. Widespread use of antibiotics in animal farming may increase antibiotic resistance, posing a serious threat to the health of farmed animals and, thus, to human food security and health.
Lnc-RPS6P3 Inhibits Influenza A Virus Replication and Attenuates the Inhibitory Effect of NS1 on Innate Immune Response
Authors: Wang Mingge , Yao Xinli , Tong Xiaomei , Qi Dandan , Ye Xin
Published: March 2024

Host factors play important roles in influenza A virus (IAV) replication. In order to identify novel host factors involved in IAV replication, we compared the differentially expressed genes in A549 cells after IAV infection. We found that lncRNA lnc-RPS6P3 was up-regulated upon viral infection and poly(I:C) and IFN-β treatment, indicating it was an interferon-stimulated gene. Functional analysis demonstrated that overexpression of lnc-RPS6P3 inhibited IAV replication while knockdown of lnc-RPS6P3 promoted viral infection in A549 cells. Lnc-RPS6P3 inhibited both transcription and replication of IAV. Further study showed that lnc-RPS6P3 interacted with viral NP and interfered with NP self-oligomerization and, consequently, inhibited vRNP activity. In addition, lnc-RPS6P3 interacted with viral NS1 and reduced the interaction of NS1 and RIG-I; it also attenuated the inhibitory effect of NS1 on IFN-β stimulation. In conclusion, we revealed that lnc-RPS6P3 is an interferon-stimulated gene that inhibits IAV replication and attenuates the inhibitory effect of NS1 on innate immune response.
An Untargeted Metabolomic Analysis of Lacticaseibacillus (L.) rhamnosus, Lactobacillus (L.) acidophilus, Lactiplantibacillus (L.) plantarum and Limosilactobacillus (L.) reuteri Reveals an Upregulated Production of Inosine from L. rhamnosus
Authors: Spaggiari Luca , Pedretti Natalia , Ricchi Francesco , Pinetti Diego , Campisciano Giuseppina , De Seta Francesco , Comar Manola , Kenno Samyr , Ardizzoni Andrea , Pericolini Eva
Published: March 2024

Lactic acid bacteria are considered an inexhaustible source of bioactive compounds; indeed, products from their metabolism are known to have immunomodulatory and anti-inflammatory activity. Recently, we demonstrated that Cell-Free Supernatants (CFS) obtained from Lactobacillus (L.) acidophilus, Lactiplantibacillus (L.) plantarum, Lacticaseibacillus (L.) rhamnosus, and Limosilactobacillus (L.) reuteri can impair Candida pathogenic potential in an in vitro model of epithelial vaginal infection. This effect could be ascribed to a direct effect of living lactic acid bacteria on Candida virulence and to the production of metabolites that are able to impair fungal virulence. In the present work, stemming from these data, we deepened our knowledge of CFS from these four lactic acid bacteria by performing a metabolomic analysis to better characterize their composition. By using an untargeted metabolomic approach, we detected consistent differences in the metabolites produced by these four different lactic acid bacteria. Interestingly, L. rhamnosus and L. acidophilus showed the most peculiar metabolic profiles. Specifically, after a hierarchical clustering analysis, L. rhamnosus and L. acidophilus showed specific areas of significantly overexpressed metabolites that strongly differed from the same areas in other lactic acid bacteria. From the overexpressed compounds in these areas, inosine from L. rhamnosus returned with the best identification profile. This molecule has been described as having antioxidant, anti-inflammatory, anti-infective, and neuroprotective properties. The biological significance of its overproduction by L. rhamnosus might be important in its probiotic and/or postbiotic activity.
[BMP]+[BF4]−-Modified CsPbI1.2Br1.8 Solar Cells with Improved Efficiency and Suppressed Photoinduced Phase Segregation
Authors: Xie Haixia , Li Lei , Zhang Jiawei , Zhang Yihao , Pan Yong , Xu Jie , Yin Xingtian , Que Wenxiu
Published: March 2024

With the rapid progress in a power conversion efficiency reaching up to 26.1%, which is among the highest efficiency for single-junction solar cells, organic–inorganic hybrid perovskite solar cells have become a research focus in photovoltaic technology all over the world, while the instability of these perovskite solar cells, due to the decomposition of its unstable organic components, has restricted the development of all-inorganic perovskite solar cells. In recent years, Br-mixed halogen all-inorganic perovskites (CsPbI3−xBrx) have aroused great interests due to their ability to balance the band gap and phase stability of pure CsPbX3. However, the photoinduced phase segregation in lead mixed halide perovskites is still a big burden on their practical industrial production and commercialization. Here, we demonstrate inhibited photoinduced phase segregation all-inorganic CsPbI1.2Br1.8 films and their corresponding perovskite solar cells by incorporating a 1-butyl-1-methylpiperidinium tetrafluoroborate ([BMP]+[BF4]−) compound into the CsPbI1.2Br1.8 films. Then, its effect on the perovskite films and the corresponding hole transport layer-free CsPbI1.2Br1.8 solar cells with carbon electrodes under light is investigated. With a prolonged time added to the reduced phase segregation terminal, this additive shows an inhibitory effect on the photoinduced phase segregation phenomenon for perovskite films and devices with enhanced cell efficiency. Our study reveals an efficient and simple route that suppresses photoinduced phase segregation in cesium lead mixed halide perovskite solar cells with enhanced efficiency.
Comparison of the Clinical Manifestation of HPAI H5Nx in Different Poultry Types in the Netherlands, 2014–2022
Authors: Wolters Wendy J. , Vernooij J. C. M. , Spliethof Thomas M. , Wiegel Jeanine , Elbers Armin R. W. , Spierenburg Marcel A. H. , Stegeman J. Arjan , Velkers Francisca C.
Published: March 2024

This study describes clinical manifestations of highly pathogenic avian influenza (HPAI) H5N1, H5N8 and H5N6 outbreaks between 2014 and 2018 and 2020 and 2022 in the Netherlands for different poultry types and age groups. Adult duck (breeder) farms and juvenile chicken (broiler and laying pullet) farms were not diagnosed before 2020. Outbreaks in ducks decreased in 2020–2022 vs. 2014–2018, but increased for meat-type poultry. Neurological, locomotor and reproductive tract signs were often observed in ducks, whereas laying- and meat-type poultry more often showed mucosal membrane and skin signs, including cyanosis and hemorrhagic conjunctiva. Juveniles (chickens and ducks) showed neurological and locomotor signs more often than adults. Diarrhea occurred more often in adult chickens and juvenile ducks. Mortality increased exponentially within four days before notification in chickens and ducks, with a more fluctuating trend in ducks and meat-type poultry than in layers. For ducks, a mortality ratio (MR) > 3, compared to the average mortality of the previous week, was reached less often than in chickens. A lower percentage of laying flocks with MR > 3 was found for 2020–2022 vs. 2014–2018, but without significant differences in clinical signs. This study provides a basis for improvements in mortality- and clinical-sign-based early warning criteria, especially for juvenile chickens and ducks.
A Novel Analytical Interpolation Approach for Determining the Locus of a Zoom Lens Optical System
Authors: Lee Jiwon , Ryu Jaemyung , Choi Hojong
Published: March 2024

In an optical system with multiple lens groups and increased zoom magnification levels, achieving a smooth zoom locus is increasingly difficult. Traditional methods for calculating zoom loci often involve complex and time-consuming formulas. Consequently, we utilized the Padé approximation in optical design software to compute the zoom locus analytically, irrespective of the number of zoom positions (nodes). The initial data were used to assign orders to rational function polynomials, facilitating Padé approximation. If the image surface extended beyond the depth of focus (DOF), a node was added, with adjustments made until it fell within the DOF range. Furthermore, Padé approximation was performed to prevent singularities. The loci of all lens groups in the optical system can be expressed in a rational function format. Specifically, the numerator and denominator polynomial degrees were 20° and 1°, respectively, with their sum being the total number of nodes. In addition, we calculated the zoom locus by increasing the numerator sequence to minimize the occurrence of the singularity and added the node automatically to enable zoom locus calculation in all optical systems. Accordingly, we could make fast calculations, unlike conventional methods, using complex and time-consuming simultaneous equations. Therefore, we could express the locus of the compensated group in the form of a smooth function, as presently shown.
Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes
Authors: Feng Chunda , Stetina Salliana R. , Erpelding John E.
Published: March 2024

Reniform nematode (Rotylenchulus reniformis) is an important microparasite for Upland cotton (Gossypium hirsutum L.) production. Growing resistant cultivars is the most economical management method, but only a few G. barbadense genotypes and some diploid Gossypium species confer high levels of resistance. This study conducted a transcriptome analysis of resistant genotypes to identify genes involved in host plant defense. Seedlings of G. arboreum accessions PI 529728 (A2-100) and PI 615699 (A2-190), and G. barbadense genotypes PI 608139 (GB 713) and PI 163608 (TX 110), were inoculated with the reniform nematode population MSRR04 and root samples were collected on the fifth (D5) and ninth (D9) day after inoculation. Differentially expressed genes (DEGs) were identified by comparing root transcriptomes from inoculated plants with those from non-inoculated plants. Accessions A2-100 and A2-190 showed 52 and 29 DEGs on D5, respectively, with 14 DEGs in common, and 18 DEGs for A2-100 and 11 DEGs for A2-190 on chromosome 5. On D9, four DEGs were found in A2-100 and two DEGs in A2-190. For GB 713, 52 and 43 DEGs were found, and for TX 110, 29 and 117 DEGs were observed on D5 and D9, respectively. Six DEGs were common at the two sampling times for these genotypes. Some DEGs were identified as Meloidogyne-induced cotton (MIC) 3 and 4, resistance gene analogs, or receptor-like proteins. Other DEGs have potential roles in plant defense, such as peroxidases, programmed cell death, pathogenesis related proteins, and systemic acquired resistance. Further research on these DEGs will aid in understanding the mechanisms of resistance to explore new applications for the development of resistant cultivars.
Phytochemical Screening, Antioxidant Effect and Sperm Quality of the Bomba ceiba Stamen Extracts on Charolais Cattle Sperm Induced by Ferrous Sulfate
Authors: Laoung-on Jiraporn , Ounjaijean Sakaewan , Sudwan Paiwan , Boonyapranai Kongsak
Published: March 2024

Orange Bombax ceiba (B. ceiba) is an indigenous plant, and its stamen is an important ingredient in traditional Lanna food. There are limitations in scientific reports on the effects of the biological activities of B. ceiba stamens on the male reproductive system. This study aims to investigate the phytochemical compounds of the orange B. ceiba stamen and its potential effect on the antioxidant properties and quality of cattle sperm treated with Fe. The orange BUE had the highest total phenolics, total tannins, total monomeric anthocyanins, and maximal antioxidant potential. The orange BAE had the highest concentration of total flavonoids. LC-QTOF/MS showed that the orange BUE contained the highest number of phytochemical compounds related to male reproductive enhancement. The orange BUE enhanced sperm motility, and both the orange BUE and the BAE enhanced sperm viability and normal sperm morphology via free radical scavenging. It might be suggested that B. ceiba stamens have benefits for sperm preservation, sperm quality, and increasing the economic value of local plants, and that they may be developed and used to guard against oxidative stress from cryodamage induced by frozen semen technology.