bluuebunny/crossref_metadata_2025 huggingface dataset:
Low-Energy Clay–Cement Slurries Find Application as Waterproofing Membranes for Limiting the Migration of Contaminants—Case Studies in Poland
Authors: Stempkowska Agata , Wójcik Łukasz , Ostrowski Krzysztof Adam , Gawenda Tomasz
Published: December 2022

The purpose of this review was to present clay–cement suspensions that are mainly used in the construction and renovation of hydrotechnical facilities and flood protection. We present the characteristics of clay–cement slurries that are used in waterproofing barriers. One of their significant components are clays of different types and origins. Examples of kaolin-type and smectite-type clays, which are associated minerals, as balanced components of suspensions are presented. As mentioned, before the hydration process, barriers are suspended, and the main test methods are rheometric measurements. Their rheological parameters, extremely important from the point of view of injection mechanics, are shown. The higher the flow limit and faster the reconstruction of thixotropic structures, the higher the energy input of the injection processes. After the hydration process, it is important to form agglomerates and seal the barrier; therefore, we present a summary of the strength results, filtration coefficients and microscopic images of the resulting structures. Additional properties of such barriers in terms of limiting contaminant migration are indicated. One of the aspects affecting the use of liquid slurries is their application methods; thus, a summary of low-energy slurry injection methods is also presented.
Effects of Depositional Processes in Submarine Canyons and Distribution of Gas Chimneys on Gas Hydrate Accumulation in the Shenhu Sea Area, Northern South China Sea
Authors: He Yunlong , Kuang Zenggui , Cheng Cong , Jiang Tao , Zhang Cheng , Lu Biyu , Yang Chengzhi , Liu Jiayu , Xiang Changlong
Published: December 2022

Previous gas hydrate production tests conducted by the Guangzhou Marine Geological Survey (GSGM) in 2017 and 2020 indicated the great potential of gas hydrates in the Shenhu Sea area in the Pearl River Mouth Basin (PRMB), China. In this study, the effects of deposition processes in submarine canyons and the distribution of gas chimneys on gas hydrate accumulation were investigated using high-resolution two- dimensional (2D) and three-dimensional (3D) seismic data. Four intact submarine canyons were identified in the study area. Five deepwater depositional elements are closely related to submarine canyons: lateral accretion packages (LAPs), basal lags, slides, mass transport deposits (MTDs), and turbidity lobes. MTDs and lobes with multiple stages outside the distal canyon mouth reveal that the sedimentary evolution of the canyon was accompanied by frequent sediment gravity flows. Gas chimneys originating from Eocene strata are generally up to 3 km wide and distributed in a lumpy or banded pattern. The analysis of seismic attributes confirmed fluid activity in these gas chimneys. Gas hydrates are mainly distributed in ridges among different canyons. Based on the gas sources of gas hydrates and depositional evolution of submarine canyons, depositional processes of sediment gravity flows in submarine canyons and the distribution of gas chimneys significantly affect the accumulation of gas hydrates. Based on these findings, this study establishes a conceptional model for the accumulation of gas hydrate, which can provide guidance in the prediction for favorable gas hydrates zones in the area and nearby.
Theoretical and Experimental Study of Methyl Ammonium Antimony Iodide-Based Lead-Free Perovskite Solar Cells
Authors: Alsalme Ali , Khan Rais Ahmad , Altowairqi Malak Faisal , Almuryyi Nouf AbdulRahman
Published: December 2022

Methyl ammonium antimony iodide (MA3Sb2I9) perovskite-like materials have gained enormous attention from the scientific community because of their excellent aerobic stability, good optical properties, and less-toxic nature. Herein, we report on the simulation of FTO/TiO2/MA3Sb2I9/spiro-OMeTAD via solar cell capacitance (SCAPS) software. The thickness of the absorber layer, hole-transport layer, or electron-transport layer may significantly impact the photovoltaic performance of the perovskite solar cells (PSCs). In this connection, the thickness of the absorber layer, hole-transport layer, or electron-transport layer was varied during the simulation process and the optimized PSCs demonstrated a good efficiency of 14.90% including an excellent open circuit voltage (Voc) of 1.41 V. Furthermore, we also fabricated MA3Sb2I9-based PSCs and their photovoltaic performance was evaluated. The fabricated PSCs showed a reasonably good efficiency of 1.27% and Voc of 0.51 V.
Study of the Characterisation Method of Effective Two-Phase Seepage Flow in the Construction of Gas Storage Reservoirs
Authors: Jiao Guoying , Zhu Shijie , Xie Fei , Yang Shuhe , Xiang Zuping , Xu Jiangen
Published: December 2022

During the rebuilding of a gas reservoir, repeated “strong injection and mining” processes change the seepage capacities of gas and water. Hence, accurately determining the seepage laws of gas and water in a gas storage reservoir is crucial. In this study, a standard relative permeability test was conducted with a one-dimensional core. Additionally, a gas reservoir injection and mining simulation experiment was conducted with a two-dimensional plate. The results show that the relative permeability curve obtained by the one-dimensional core test did not accurately reflect the operation characteristics of the gas storage and the change in the seepage law during the gas reservoir construction. Furthermore, in the two-dimensional plate experiment, the operation mode was restored using the plane radial flow formula, the mutual relationship between the gas and water’s effective permeability under different injection stages was established, and the multi-cycle injection operation was accurately described. This method lays the foundation for the construction of gas reservoirs and the establishment of the multi-phase seepage law for gas reservoirs.
Entropy Generation and Mixed Convection of a Nanofluid in a 3D Wave Tank with Rotating Inner Cylinder
Authors: Alsabery Ammar I. , Alshukri Mohammed J. , Jabbar Nasr A. , Eidan Adel A. , Hashim Ishak
Published: December 2022

The generation of entropy and mixed convection in a nanofluid-filled 3D wavy tank containing a rotating cylinder is investigated. The top wavy surface of the tank is heated and all vertical surfaces are assumed to be adiabatic, while the bottom horizontal surface remains isothermally cold. The tank contains a solid cylinder and is saturated with an Al2O3–water nanofluid. The numerical simulations using the FEM are performed for the Richardson number (0.01≤Ri≤100), nanoparticle volume fraction (0≤ϕ≤0.04) and number of oscillations (0≤N≤4). The numerical results of the present work are given in terms of 3D streamlines, isotherms and local entropy generation, as well as average heat transfer and Bejan number. The results show that for low values of the Richardson number and oscillation, heat transfer enhancement can be achieved by increasing the nanoparticle volume fraction.
Machine Learning Assisted Chemical Process Parameter Mapping on Lignin Hydrogenolysis
Authors: Liu Yin , Cheng Shuo , Cross Jeffrey Scott
Published: December 2022

Lignin depolymerization has been studied for decades to produce carbon-neutral chemicals/biofuels and biopolymers. Among different chemical reaction pathways, catalytic hydrogenolysis favors reactions under relatively mild conditions, while its yield of bio-oil and high-value aromatic products is relatively high. In this study, the influence of reaction parameters on lignin hydrogenolysis are discussed by chemical process parameter mapping and modeled using three different machine learning algorithms based upon literature experimental data. The best R2 scores for solid residue and aromatic yield were 0.92 and 0.88 for xgboost, respectively. The parameter importance was examined, and it was observed that lignin-to-solvent ratio and average pore size have a larger impact on lignin hydrogenolysis results. Finally, the optimal conditions of lignin hydrogenolysis were predicted by chemical process parameter mapping using the best-fit machine learning model, which indicates that further process improvements can potentially generate higher yields in industrial applications.
DFT Prediction of Radiolytic Stability of Conformationally Flexible Ligands
Authors: Smirnova Anastasiia , Yablonskiy Maksim , Petrov Vladimir , Mitrofanov Artem
Published: December 2022

Radiolytic stability is one of the main requirements of the ligands for the reprocessing of spent nuclear fuel. The prediction of radiation stability based only on the 2D molecular structural formula allows us to accelerate and simplify the development of new ligands. Here, we used quantum chemistry to investigate the radiolytic behavior of water-soluble diglycolamides as one of the most popular ligands for spent nuclear fuel reprocessing. The accurate accounting of conformational mobility in the descriptors based on the Frontier Orbital Fukui theory allowed us to obtain a good correlation between theoretical and experimental data.
Computed Tomography Observation and Image-Based Simulation of Fracture Propagation in Compressed Coal
Authors: Wang Zhaohui , Sun Wenchao , Shui Yanting , Liu Pengju
Published: December 2022

In this study, the fracture propagation characteristics and associated mechanisms of coal are investigated by using computed tomography (CT) observation and image-based simulation. The spatial distribution and the structural morphology of original fractures provide significant influences on the failure behavior of fractured coal. The fractures with small dip angles and large openings result in more-obvious fracture closure and stable propagation stages, while failure pattern is more sensitive to those with large dip angles. The coal tends to experience brittle failure, which transits from a splitting to mixed-splitting faulting mode because of the difference in original fracture distribution. The final failure fracture network originates mainly from the propagation of original fractures, driven by localized tensile stress. Fracture interaction and mineral influence tend to increase the complexity in the failure fracture network. Moreover, image-based numerical models are established on the basis of CT reconstruction, where the spatial distribution and the structural morphology of original fractures are properly considered. Numerical modeling reproduces similar stress–strain responses and failure fracture networks to that observed in the experiment. The predicted distribution of tensile stress shows a similar evolution trend to the failure fracture network, implying that the fracture propagation of coal is dominated by tensile failure. Shear cracks emerge mainly after the large fracture running through the coal sample has been formed.
A Tabulated Chemistry Multi-Zone Combustion Model of HCCI Engines Supplied with Pure Fuel and Fuel Blends
Authors: De Bellis Vincenzo , Malfi Enrica , Lanotte Alfredo , De Felice Massimiliano , Teodosio Luigi , Bozza Fabio
Published: December 2022

Homogeneous charge compression ignition is considered a promising solution to face the increasing regulations imposed by the legislator in the transport sector, thanks to pollutant and CO2 emissions reduction. In this work, a quasi-dimensional multi-zone HCCI model integrated with 1D commercial software is developed and validated. It is based on the control mass Lagrangian approach and computes the mixture chemistry evolution through offline tabulation of chemical kinetics (tabulated kinetic of ignition). Thus, the simulation can predict mixture auto-ignition with reduced computational effort and high accuracy. Multi-zone schematization mimics the typical thermal stratification of HCCI engines, controlling the combustion evolution. The model is coupled to sub-models for pollutant emissions estimation. Initially, the tabulated chemistry approach is validated against a chemical kinetics solver applied to a constant-volume homogeneous reactor, considering various fuel blends. The model is then used to simulate the operations of four engines using different fuels (hydrogen, methane, n-heptane, and n-heptane/toluene/ethanol blend), under various boundary conditions. The model predictivity is demonstrated against pressure traces, heat release rate, and noxious emissions. The numerical results showed to adequately agree with measured counterparts (average relative error of 1.3% on in-cylinder pressure peak, average absolute error of 0.95 CAD on pressure peak angle, average relative error of 8.4% on uHCs emissions, absolute error below 1 ppm on NOx emissions) only adapting the thermal stratification to the engines under study. The methodology proved to be a reliable tool to investigate the operation of an HCCI engine, applicable in the development of new engine architecture.
Recent Advances and Challenges toward Efficient Perovskite/Organic Integrated Solar Cells
Authors: Hong Soonil , Lee Jinho
Published: December 2022

Recently, emerging third-generation photovoltaic technologies have shown rapid progress in device performance; the power conversion efficiencies (PCEs) of organic bulk heterojunction (BHJ) and perovskite solar cells (PSCs) are now surpassing 19% and 25%, respectively. Despite this dramatic enhancement, their efficiencies are theoretically limited based on the detailed balance model which accounts for inevitable loss mechanisms under operational conditions. Integrated solar cells, formed by monolithically integrating two photoactive layers of perovskite and BHJ with complementary absorption, provide a promising platform for further improvement in solar cell efficiency. In perovskite/BHJ integrated solar cells (POISCs), high bandgap perovskite offers high open-circuit voltage with minimal losses while low bandgap organic BHJ extends absorption bandwidth by covering the near-infrared region, resulting in additional photocurrent gain. Different from conventional tandem solar cells, integrated solar cells contain merged photoactive layers without the need for complicated recombination layers, which greatly simplifies fabrication processes. In this review, we summarize the recent progress in POISCs, including operational mechanism and structural development, and remaining challenges on the road toward efficient devices.
A Deep Understanding of Romanian Attitude and Perception Regarding Nuclear Energy as Green Investment Promoted by the European Green Deal
Authors: Tantau Adrian , Puscasu Greta Marilena , Cristache Silvia Elena , Alpopi Cristina , Fratila Laurentiu , Moise Daniel , Ciobotar Georgeta Narcisa
Published: December 2022

The analysis of public attitudes towards nuclear energy represents an issue that is commonly investigated, especially considering the new context of classifying some nuclear power plants as green investments under the European Green Deal. The importance of this topic is critical to the future of nuclear power generation. The purpose of this study is to identify the relationships that exist between the different factors and the attitude of the public towards nuclear energy in the context of the European Green Deal. The article identifies and analyzes the main factors that influence this relationship. In this context, a questionnaire-based survey was conducted regarding the identification of the relationship between public knowledge, degree of cooperation, perceived risks, trust and attitude towards nuclear energy. The sample was chosen using the snowball method. The analysis was made up of 578 respondents from different segments of age, gender, place of residence, field of activity, work experience and income. Following the survey, the main conclusion was that over 30% of the respondents do not want nuclear energy and are adamantly opposed to it. At the same time, the results indicate that the respondents’ attitude is not directly influenced by the risks they perceive in relation to nuclear energy.
A Bidirectional Modular Cuk-Based Power Converter for Shore Power Renewable Energy Systems
Authors: Darwish Ahmed
Published: December 2022

Supplying shipping vessels with electricity at the ports can improve the air quality of the ports, reduce the greenhouse gas emissions from the shipping industry, contribute to the economic growth, and increase the political dependency of the countries by reducing the dependence on conventional fossil fuels. Several countries promote supplying the vessels when they are docking at ports from renewable energy systems by establishing dedicated funding mechanisms to remove the obstacles facing the shore power systems. In this context, this paper presents a new modular power electronic converter for shore power systems at shipping ports, which can perform three functions. Firstly, it will harvest the energy from a renewable energy source, such as hydroelectric and solar photovoltaic (PV) sources and ensure maximum energy extraction. Secondly, it will control the power flow from these sources to the battery storage. Finally, it will control the power flow from the battery to the vessels and/or the utility grid when necessary. A current-source converter based on isolated Cuk converter is used as the submodule (SM) of the proposed modular converter due to several features. The Cuk SM can provide high efficiency, minimised dc capacitance, and flexible output voltage higher or lower than the input voltage from the PV modules. To verify the mathematical analyses and computer simulations, experimental results are obtained from a small-scale modular prototype controlled by a TMSF28335 DSP.
Biotechnological Production of Optically Pure 2,3-Butanediol by Bacillus subtilis Based on Dissolved Oxygen Control Strategy
Authors: Suttikul Suthkamol , Charalampopoulos Dimitris , Chatzifragkou Afroditi
Published: December 2022

2,3-Butanediol (2,3-BD) is a promising platform chemical, produced from microbial cells. Oxygen availability is a crucial factor driving the formation and proportion of 2,3-BD and acetoin in 2,3-BD producing bacterial strains. In this study, the ability of B. subtills GD5 to produce 2,3-BD in optimized sucrose-based media was evaluated, by investigating the impact of carbon to nitrogen (C/N) ratio and the effectiveness of alternative low-cost nitrogen sources (corn steep liquor, soybean meal, and ammonium sulphate). Subsequently, different dissolved oxygen (DO) controlling regimes were assessed in batch bioreactor fermentations. The best fermentation outcomes were obtained with uncontrolled DO, achieving 5.88 g/L of optically pure (R,R)-2,3-BD (~100% purity), accompanied by a production yield of 0.43 g/g, and a productivity of 0.2 g/L/h. Additionally, the influence of the DO controlling regime on B. subtills key enzymes involved in the reverse activity of acetoin reductase was also monitored. A fed-batch process under the most suitable DO conditions was carried out to improve 2,3-BD production, achieving 42.31 g/L 2,3-BD with a production yield of 0.52 g/g. Thus, B. subtilis GD5 is a promising strain for the efficient production of pure chiral (R,R)-2,3-BD under uncontrolled DO conditions, using alternative low-cost nitrogen sources.
Comparative Analysis of Rumen Bacterial Profiles and Functions during Adaption to Different Phenology (Regreen vs. Grassy) in Alpine Merino Sheep with Two Growing Stages on an Alpine Meadow
Authors: Gao Xiang , Wang Hucheng
Published: December 2022

Phenological periods can affect the growth of forage, the single food source for grazing animals, and its nutrition and the stage of growth of the animals can affect the rumen microbiota. The aim of this study was to evaluate the effects of phenological periods (regreen vs. grassy) and growing stages (hoggets (1.5 years old) vs. rams (3 years old)) on rumen bacteria communities and functions in Alpine Merion sheep. The result showed that the Alpha diversity indices of ACE (p < 0.001), Chao (p < 0.001), and Shannon (p < 0.001) were higher in the regreen stage. At the phylum level, the abundances of Bacteroidetes (p = 0.003) and Firmicutes (p = 0.017) shifted with plant phenology. The abundance of fiber-degrading bacteria altered at the genus level (p < 0.05). Genes related to fatty acid degradation and metabolism increased in both the growing stage sheep (p < 0.05). In the grassy stage, the abundance of vitamin B6 metabolism (p = 0.046) was increased in hoggets. In summary, this study showed that the phenological stage had a significant effect on the rumen bacterial compartment and functions in two growing stages, while the growing stage only tended to change rumen bacterial diversity.
Effect of Live Yeasts (Pichia guilliermondii) on In Vitro Fermentation of Corn Stover as a Fibrous Substrate
Authors: González Mora Bexy , Ruiz-Barrera Oscar , Castillo Rangel Francisco , Castillo-Castillo Yamicela
Published: December 2022

The objective of the present study was to examine the potential utilization of the yeast strain Pichia guilliermondii (Levica 27) on the ruminal fermentation of corn stover. Two in vitro treatments were evaluated: (A) filtered rumen liquid + powdered corn stover + yeast-free culture medium and (B) filtered rumen liquid + powdered corn stover + culture medium inoculated with yeast. Samples were collected at 0, 6, 12, and 24 h and examined for effects on culture pH, ammonia nitrogen (NH3-N), the fiber digestibility percentages of neutral detergent fiber and acid detergent fiber, the digestibility of dry matter, and the concentration of volatile fatty acids (VFAs). The results showed that the addition of Levica 27 had no effect on the ruminal pH, NH3-N or the digestibility indicators (p > 0.05). However, at 12 h, the inclusion of Levica 27 increased (p < 0.05) the molar concentrations of total VFAs and acetic and propionic acids in ruminal fluid and decreased (p < 0.05) the C2:C3 ratio. It is concluded that Levica 27 seems to have a stimulating effect on ruminal fermentation over a range within 6 and 12 h, a result that should be taken into account when in vivo studies are performed with this yeast strain.
Limosilactobacillus reuteri Regulating Intestinal Function: A Review
Authors: Jiang Jiayan , Li Ke , Xiao Yu , Zhong Aiai , Tang Jiaojiao , Duan Yufan , Li Zongjun
Published: December 2022

Probiotics have extensive use in daily life, due to the function of the changing intestinal metabolism and material conversion processes, wherein they remodel the intestinal microbiota, regulate the intestinal function and affect the organism’s health. Limosilactobacillus reuteri (L. reuteri), originally discovered in breast milk and currently reported to be present within the gut of almost all vertebrates and mammals, is an intestinal probiotic with prebiotic efficacy. Most L. reuteri have good intestinal colonization and bacteriocin secretion abilities, which can increase the expression of the mucin (mucoprotein) genes 2 MUC2 and MUC13, which in turn promote the development and maturation of intestinal organoids, and augment mucin secretion. In enteritis patients, L. reuteri downregulates α Tumor necrosis factor-α, (TNF-α), Interleukin-6 (IL-6), IL-8, and IL-12 expression to attenuate inflammation. It also induces the host’s production of immunoglobulin A (IGA), which manipulates the intestinal microbial community, inhibiting the growth of pathogens. L. reuteri has been widely used in daily life. with in-depth studies having been conducted on the prebiotic effects of L. reuteri. However, the complexity of its application in a clinical setting is still unclear because the pathogenesis of various diseases still requires a large amount of data and theoretical support.
Selection and Verification of Reference Genes for Gene Expression Studies in Different Cell Lines of Golden Pompano (Trachinotus ovatus)
Authors: Zhao Na , Zhang Han , Zhu Lin , Hou Yongwei , Wu Ying , Cao Zhenjie , Sun Yun
Published: December 2022

The golden pompano snout (GPS) and head kidney (GPHK) cell lines have been proven to be meaningful tools for the study on pathogenic infections in vitro. In this study, we aimed to select the most stable reference genes from seven housekeeping genes (Actin, B2M, GAPDH, RPL13, EF1A, 18S and UBCE) applied to two cell lines of golden pompano (GPS and GPHK) under both normal physiological conditions and stimulated conditions of the lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid (Poly I:C) relying on quantitative real-time PCR (qRT-PCR). Additionally, the raw Ct value resulting from the qRT-PCR was analyzed by the geNorm, NormFinder and BestKeeper algorithm, and the results indicated that expression for all candidate genes exhibited some discrepancy under different experimental conditions or cell types. As for the non-stimulated group, 18S and RPL13 were the most appropriate reference genes in GPS and GPHK cells, respectively. Nevertheless, the most suitable reference genes in GPS and GPHK cells, under the stimulation of LPS, were RPL13 and 18S, respectively, whereas after being stimulated with Poly I:C, UBCE and EF1A were recommended as the optimal candidates for GPS and GPHK cells, respectively. To be sure of the reliability of the selected reference genes, immune-related genes (ISG15, BCL2, IRF1 and IRF7) were chosen as target genes to normalize. The study will provide a direction for various golden pompano cell lines to screen appropriate reference genes, and will set the stage for the application of these cell lines in relevant research areas.
Moringa oleifera and Azadirachta indica Leaves Enriched Diets Mitigate Chronic Oxyfluorfen Toxicity Induced Immunosuppression through Disruption of Pro/Anti-Inflammatory Gene Pathways, Alteration of Antioxidant Gene Expression, and Histopathological Alteration in Oreochromis niloticus
Authors: Ibrahim Rowida E. , Ghamry Heba I. , Althobaiti Saed Ayidh , Almalki Daklallah A. , Shakweer Medhat S. , Hassan Mona A. , Khamis Tarek , Abdel-Ghany Heba M. , Ahmed Shaimaa A. A.
Published: December 2022

Our goal in this study was to determine the effect of dietary supplementation with Moringa oleifera (M. oleifera), and Azadirachta indica (A. indica) leaves in mitigating the effects of chronic oxyfluorfen (OXY) toxicity on the health status, expressions of immune and antioxidant genes, and tissue morphological alterations in Oreochromis niloticus. In this study, we used 370 healthy O. niloticus (average weight = 25.35 ± 0.29 g). We used 70 fish to study the 96 h lethal concentration 50 (LC50) of OXY. We assigned another 300 fish into six equal groups with five replicates (50 fish/group, 10 fish/replicate) to determine the chronic OXY toxicity for 60 days. The 96 h LC50 of OXY for O. niloticus was 6.685 mg/L. Exposure to 1/10 96 h LC50 of OXY (0.668 mg/L) had health impacts and pathological changes in the main tissues. In addition, the expressions of oxidant and immune genes were disrupted. Dietary supplementation with both M. oleifera and A. indica efficiently mitigated the toxic effects of OXY in the treated groups. Comparing the palliative efficiency of M. oleifera and A. indica, the results showed that M. oleifera was more potent in alleviating the toxic effects of OXY.
Evaluation of SPH and FVM Models of Kinematically Prescribed Peristalsis-like Flow in a Tube
Authors: Liu Xinying , Harrison Simon M. , Cleary Paul W. , Fletcher David F.
Published: December 2022

Peristaltic flow is important in many biological processes, including digestion, and forms an important component of any in silico model of the stomach. There is a clear need to verify the simulations of such flows. An analytical solution was identified that can be used for model verification, which gives an equation for the net volumetric flow over a cycle for an applied sinusoidal wall motion. Both a smooth particle hydrodynamics (SPH) code (from the CSIRO), which is being used to develop a stomach model that includes wall motion, buoyancy, acid secretion and food breakdown, and the Ansys Fluent Finite Volume Method (FVM) solver, that is widely used in industry for complex engineering flows, are used in this exercise. Both give excellent agreement with the analytic solution for the net flow over a cycle for a range of occlusion ratios of 0.1–0.6. Very similar velocity fields are obtained with the two methods. The impact of parameters affecting solution stability and accuracy are described and investigated. Having validated the moving wall capability of the SPH model it can be used with confidence in stomach simulations that include wall motion.
Cancer Exosomes: An Overview and the Applications of Flow
Authors: Bryant Parker , Sikavitsas Vassilios I.
Published: December 2022

Cancer is one of the most prevalent and disruptive diseases affecting the population, and as such, is the subject of major research efforts. Recently, these efforts have been put towards understanding the role that exosomes can play in the progression of cancer. Exosomes are small extracellular vesicles ranging from 40–150 nm in size that carry bioactive molecules like proteins, DNA, RNA, miRNA, and surface receptors. One of the most important features of exosomes is their ability to easily travel throughout the body, extending the reach of parent cell’s signaling capabilities. Cancer derived exosomes (CDEs) carry dangerous cargo that can aid in the metastasis, and disease progression through angiogenesis, promoting epithelial to mesenchymal transition, and immune suppression. Exosomes can transport these molecules to cells in the tumor environment as well as distant premetastatic locations making them an extremely versatile tool in the toolbelt of cancer. This review aims to compile the present knowledge and understanding of the involvement of exosomes in the progression of cancer as well as current production, isolation, and purification methods, with particular interest on flow perfusion bioreactor and microfluidics systems, which allow for accurate modeling and production of exosomes.
Detailed Investigation of the Droplet Dynamics Parameters Produced by Artificially Induced Bag-Breakup Fragmentation
Authors: Sergeev Daniil , Kandaurov Alexander , Vdovin Maksim , Troitskaya Yuliya
Published: December 2022

This paper presents the results of detailed studies of the processes of droplet formation and its characteristics under conditions of artificially induction of a bag-breakup fragmentation event. A shadow imaging method was used in combination with the high-speed video filming of the side-view fragmentation process. Trajectories and ejection velocity characteristics of the formed droplets are determined by identifying particles in consecutive frames with combined use of Particle Imaging Velocimetry (PIV) and Particle Tracking Velocimetry (PTV). Based on the results of trajectory processing, the distributions of droplet velocities for the selected regions are obtained, and estimates of the ejection velocities at various heights are proposed.
Rapid and High-Throughput Determination of Sixteen β-agonists in Livestock Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC-MS/MS
Authors: Yan Yonghong , Ning Jun , Cheng Xin , Lv Qingqin , Teng Shuang , Wang Wei
Published: December 2022

β-agonists are illegally added to animal feed because they can greatly increase carcasses’ leanness, which impairs the safety of animal-derived foods and indirectly endangers human health. This study aimed to develop an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for determining sixteen β-agonists in livestock meat. The homogenized samples were subjected to enzymatic hydrolysis using β-glucuronidase/sulfatase at 40 °C for 2 h, extracted with acetonitrile containing 1% acetic acid (v/v), and purified by the one-step Qvet-AG extraction column. The residue was redissolved by 0.1% aqueous formic acid/methanol (9:1, v/v) after blow-drying by nitrogen, and then determined by UHPLC-MS/MS. The results demonstrated that the well linearity was in the range of 0.1–50 μg/L with the correlation coefficient (R2) ≥0.9928, and the limits of detection (LOD) and quantification (LOQ) were 0.01–0.11 μg/kg and 0.04–0.38 μg/kg, respectively. With intraday and interday relative standard deviations (RSDs) being less than 10%, the average recoveries of pork, beef, and lamb at various spiked levels ranged from 62.62–115.93%, 61.35–106.34%, and 62.00–111.83%, respectively. In conclusion, the established method is simple, efficient, sensitive, and suitable for the simultaneous detection of several β-agonist residues in livestock meat.
Effect of Tea Polyphenols on the Storage Stability of Non-Fermented Frozen Dough: Protein Structures and State of Water
Authors: Zheng Kai , Chen Zhehan , Fu Yang , Chen Lei , Zhu Xiangwei , Chen Xi , Ding Wenping
Published: December 2022

The usage of tea polyphenols (TPs) as a natural food additive into non-fermented frozen dough (NFFD) has rarely been investigated, and results have been controversial. Hence, this study investigated the effect of TPs at various levels (0, 0.5, 1, and 2%) on the quality of NFFD stored from 0 to 4 weeks. The rheological characteristics, water state, protein, and its microstructure were analyzed by DSC, LF-NMR, SDS-PAGE, FT-IR, and SEM, respectively. Results showed that the 0.5% TP group delayed the deterioration of protein and inhibited the water migration in dough throughout the whole frozen storage period. In addition, the 0.5% TP group enhanced the rheological properties of NFFD and stabilized the sulfhydryl content and the secondary structure in the gluten network. On the contrary, opposite phenomena were found in the 1 and 2% TP groups, which might be due to the induction of excess hydroxyl groups from TPs. In conclusion, our results suggested that a proper addition of TPs, but not an excessive amount (>1%), exhibited beneficial effects in maintaining the quality of NFFD during the 4-week frozen storage. Moreover, this paper elucidated the mechanism of TPs in influencing the protein structure and water state of NFFD during storage and provided new insight into its application in dough-based foods.
Asparagus Fructans as Emerging Prebiotics
Authors: Hamdi Amel , Viera-Alcaide Isabel , Guillén-Bejarano Rafael , Rodríguez-Arcos Rocío , Muñoz Manuel Jesús , Monje Moreno Jose Manuel , Jiménez-Araujo Ana
Published: December 2022

Commercial fructans (inulin and oligofructose) are generally obtained from crops such as chicory, Jerusalem artichoke or agave. However, there are agricultural by-products, namely asparagus roots, which could be considered potential sources of fructans. In this work, the fructans extracted from asparagus roots and three commercial ones from chicory and agave were studied in order to compare their composition, physicochemical characteristics, and potential health effects. Asparagus fructans had similar chemical composition to the others, especially in moisture, simple sugars and total fructan contents. However, its contents of ash, protein and phenolic compounds were higher. FTIR analysis confirmed these differences in composition. Orafti®GR showed the highest degree of polymerization (DP) of up to 40, with asparagus fructans (up to 25) falling between Orafti®GR and the others (DP 10–11). Although asparagus fructan powder had a lower fructan content and lower DP than Orafti®GR, its viscosity was higher, probably due to the presence of proteins. The existence of phenolic compounds lent antioxidant activity to asparagus fructans. The prebiotic activity in vitro of the four samples was similar and, in preliminary assays, asparagus fructan extract presented health effects related to infertility and diabetes diseases. All these characteristics confer a great potential for asparagus fructans to be included in the prebiotics market.
Green-Labelled Rice versus Conventional Rice: Perception and Emotion of Chinese Consumers Based on Review Mining
Authors: Xu Heng , Xiao Mengyun , Zeng Jun , Hao Huihui
Published: December 2022

The COVID-19 pandemic increased public health awareness, changing consumers’ sensitivity and beliefs about food health. Food anxiety and health scares turn consumers toward safe and healthy foods to strengthen their immunity, which makes green food more popular. However, it remains unclear how to understand the gap between consumer intention to purchase green food and their actual purchasing behaviour. Taking rice as an object of study, comparing differences in consumer perceptions and emotions towards green-labelled rice and conventional rice is beneficial for understanding the components and psychological characteristics of consumer perceptions of green food. Therefore, we used topic modelling and sentiment analysis to explore consumers’ focus of attention, attitudinal preferences, and sentiment tendencies based on the review (n = 77,429) from JD.com. The findings revealed that (1) consumers’ concerns about green-labelled rice are increasing rapidly, and most have a positive attitude; (2) consumers of green-labelled rice are more concerned about origin, aroma, and taste than conventional rice; (3) consumers of conventional rice are more concerned about the cost-performance ratio, while consumers of green-labelled rice are also price-sensitive; (4) green label mistrust and packaging breakage during logistics are the leading causes of negative emotions among consumers of green-labelled rice. This study provides a comparative analysis of consumer perceptions and emotions between the two types of rice, thus revealing the main influencing factors of the intention-behaviour gap and providing valuable consumer insights for the promotion of green consumption and the sustainable development of the green food industry.
Enrichment of Wholemeal Rye Bread with Plant Sterols: Rheological Analysis, Optimization of the Production, Nutritional Profile and Starch Digestibility
Authors: Makran Mussa , Cilla Antonio , Haros Claudia , Garcia-Llatas Guadalupe
Published: December 2022

Bread is one of the staple foods of many countries, and its enrichment with bioactive compounds is trending. This phenomenon is focused on breads with a good nutritional profile, such as wholemeal rye bread (WRB), in which enrichment with plant sterols (PSs) is allowed in accordance with European regulations. The objective of the present study was to optimize the production of a WRB enriched with PS (PS-WRB) and to evaluate the proximate composition and starch digestibility as an indicator of nutritional quality. The rheological analysis showed that the bread dough presents satisfactory farinographic properties (dough development time 6 min; stability 4 min; degree of softening 100 Brabender units) but high water absorption (67%). The PS-WRB is high in dietary fiber and low in protein (20.4 and 7.7% w/w, dry basis, respectively) compared with other cereals reported in the scientific literature. In turn, a low starch proportion was hydrolyzed during the simulated digestion (59.9% of total starch), being also slowly hydrolyzed, as deduced from the rapidly digestible starch value (56.5% of total starch). In conclusion, WRB is a suitable matrix for PS enrichment, which allows for obtaining a product with a good nutritional profile and potential health benefits.
Alleviation Effects of Microbial Metabolites from Resveratrol on Non-Alcoholic Fatty Liver Disease
Authors: Guo Jingling , Wang Pan , Cui Yifan , Hu Xiaosong , Chen Fang , Ma Chen
Published: December 2022

Resveratrol (RSV), a polyphenolic stilbene, has been widely studied for its protective effects against non-alcoholic fatty liver disease (NAFLD) by modulating intestinal microbiota. The microbial metabolites after RSV supplement would contribute to the bioeffects of RSV, while their impacts on NAFLD were unclear. Therefore, this study aimed to investigate the beneficial effects of the main microbial metabolites from RSV on lipid metabolism by combining in vitro and in vivo models. The mice were fed a high-fat diet and injected with RSV, 3-hydroxyphenyl propionic acid (3-HPP), and 4-HPP for 13 weeks (n = 6). Body weight, serum parameters, histological analysis, and gene expression involved in lipid metabolism were quantified. Our results suggested that 100 μM of 3-HPP and 4-HPP inhibited lipid accumulation more significantly than parent RSV in an oleic acid-induced HepG2 cell line. Furthermore, 3-HPP, 4-HPP, and RSV effectively reduced liver weight and body weight, improved hepatic steatosis, and alleviated systemic inflammation in NAFLD mice. In addition, the results of quantitative real-time PCR showed that 3-HPP and 4-HPP altered the expression of cholesterol influx and efflux genes to a stronger extent than RSV. These results indicate that 3-HPP and 4-HPP are effective in regulating hepatic lipid metabolism.
In Vitro and In Situ Characterization of Psychrotrophic Spoilage Bacteria Recovered from Chilled Chicken
Authors: Wang Xinxia , Wang Zaitian , Sun Zhilan , Wang Daoying , Liu Fang , Lin Lin
Published: December 2022

Spoilage bacteria play a remarkable role in the spoilage of chilled chicken. In this paper, a total of 42 isolates belonging to 16 species of four genera were isolated from chilled chicken and displayed different characterizations of psychrotrophic spoilage. Six isolates of J7, J8, Q20, Q23, R1, and R9 with differences in proteolytic capabilities were further characterized for in situ spoilage potential evaluation. Pseudomonas lundensis J8 exhibited the strongest spoilage potential in situ, displaying a fast growth rate, increased pH velocity, high total volatile basic nitrogen, and high peptide content in the chicken samples. The volatile flavor analysis of chicken samples via electronic nose indicated that the content of characteristic odors representing spoilage, including sulfides, organic sulfide, and hydride, increased during storage. Additionally, the principle component and correlation analyses revealed that the spoilage odors produced by different species of bacteria were significantly different and positively correlated with the results of protease activity in vitro. The characteristics of spoilage bacteria in chilled chicken provided a comprehensive insight into microbial assessment during storage.
Reduction of Ethyl Carbamate in an Alcoholic Beverage by CRISPR/Cas9-Based Genome Editing of the Wild Yeast
Authors: Jung Jin-Young , Kang Min-Ji , Hwang Hye-Seon , Baek Kwang-Rim , Seo Seung-Oh
Published: December 2022

Ethyl carbamate (EC) is a naturally occurring substance in alcoholic beverages from the reaction of ethanol with urea during fermentation and storage. EC can cause dizziness and vomiting when consumed in small quantities and develop kidney cancer when consumed in excess. Thus, the reduction of EC formation in alcoholic beverages is important for food safety and human health. One of the strategies for reducing EC contents in alcoholic beverages is developing a new yeast starter strain to enable less formation of EC during fermentation. In this study, we isolated a polyploid wild-type yeast Saccharomyces cerevisiae strain from the Nuruk (Korean traditional grain-based inoculum of wild yeast and mold) and developed a starter culture by genome engineering to reduce EC contents in alcoholic beverages. We deleted multiple copies of the target genes involved in the EC formation in the S. cerevisiae by a CRISPR/Cas9-based genome editing tool. First, the CAR1 gene encoding for the arginase enzyme responsible for the formation of urea was completely deleted in the genome of S. cerevisiae. Additionally, the GZF3 gene encoding the transcription factor controlling expression levels of several genes (DUR1, 2, and DUR3) related to urea absorption and degradation was deleted in S. cerevisiae to further reduce the EC formation. The effects of gene deletion were validated by RT-qPCR to confirm changes in transcriptional levels of the EC-related genes. The resulting strain of S. cerevisiae carrying a double deletion of CAR1 and GZF3 genes successfully reduced the EC contents in the fermentation medium without significant changes in alcohol contents and fermentation profiles when compared to the wild-type strain. Finally, we brewed the Korean traditional rice wine Makgeolli using the double deletion strain of S. cerevisiae dCAR1&GZF3, resulting in a significant reduction of the EC content in Makgeolli up to 41.6% when compared to the wild-type strain. This study successfully demonstrated the development of a starter culture to reduce the EC formation in an alcoholic beverage by CRISPR/Cas9 genome editing of the wild yeast.
Biotechnological Production of Sustainable Microbial Proteins from Agro-Industrial Residues and By-Products
Authors: Bajić Bojana , Vučurović Damjan , Vasić Đurđina , Jevtić-Mučibabić Rada , Dodić Siniša
Published: December 2022

Microbial proteins, i.e., single-cell proteins or microbial biomass, can be cultivated for food and animal feed due to their high protein content and the fact that they represent a rich source of carbohydrates, minerals, fats, vitamins, and amino acids. Another advantage of single-cell proteins is their rapid production due to the growth rate of microorganisms and the possibility of using agro-industrial waste, residues and by-products for production through this renewable technology. Agro-industrial residues and by-products represent materials obtained from various processes in agriculture and agriculture-related industries; taking into account their composition and characteristics, as well as vast amounts, they have an enormous potential to generate sustainable bioproducts, such as microbial proteins. This review aims to summarize contemporary scientific research related to the production of microbial proteins on various agro-industrial residues and by-products, as well as to emphasize the current state of production of single-cell proteins and the importance of their production to ease the food crisis and support sustainable development.
Comparative Study on Physicochemical and Nutritional Qualities of Kiwifruit Varieties
Authors: Yuan Xinyu , Zheng Hao , Fan Jiangtao , Liu Fengxia , Li Jitao , Zhong Caihong , Zhang Qiong
Published: December 2022

In order to study the physicochemical and nutritional characteristics of kiwifruit varieties, 14 kiwifruits from different species with different flesh colors were selected for research. The pectin content was significantly higher in green-fleshed kiwifruits than those in red-fleshed and yellow-fleshed kiwifruits. Red-fleshed kiwifruits had the highest total flavonoid content, and green-fleshed kiwifruits in A. eriantha had the highest chlorophyll a content, chlorophyll b content and total carotenoid content. The energy and carbohydrate contents of yellow-fleshed kiwifruits were significantly lower than those of red-fleshed kiwifruit. Moreover, the protein contents in A. chinensis and A. chinensis var. deliciosa were higher than those in other species. The content of vitamin C in A. eriantha was far higher than in other kiwifruits. Red-fleshed kiwifruits had a significantly higher vitamin E and vitamin B1 content than green-fleshed kiwifruits. In addition, 1-pentanol, trans-2-hexen-1-ol, n-hexane and styrene presented only in red-fleshed kiwifruits. Therefore, these could be used as a characteristic fragrance for red-fleshed kiwifruits. Moreover, the varieties were ranked comprehensively by principal component analysis (PCA), among which the top four highest-ranking kiwifruits among the 14 varieties were ‘Huate’, ‘MHYX’, ‘Jinkui’ and ‘Xuxiang’, respectively. This study provides a reference for consumers and markets on quality improvement and processing.