bluuebunny/crossref_metadata_2025 huggingface dataset:
Potato Chip-Like 0D Interconnected ZnCo2O4 Nanoparticles for High-Performance Supercapacitors
Authors: Mallem Siva Pratap Reddy , Koduru Mallikarjuna , Chandrasekhar Kuppam , Prabhakar Vattikuti S. V. , Manne Ravi , Reddy V. Rajagopal , Lee Jung-Hee
Published: April 2021

Zinc cobaltite (ZnCo2O4) is an emerging electrode material for supercapacitors due to its rich redox reactions involving multiple oxidation states and different ions. In the present work, potato chip-like 0D interconnected ZnCo2O4 nanoparticles (PIZCON) were prepared using a solvothermal approach. The prepared material was characterized using various analytical methods, including X-ray powder diffraction and scanning electron microscopy. The possible formation mechanism of PIZCON was proposed. The PIZCON electrode material was systematically characterized for supercapacitor application. The areal capacitance of PIZCON was 14.52 mF cm−2 at 10 µA cm−2 of current density, and retention of initial capacitance was 95% at 250 µA cm−2 following 3000 continuous charge/discharge cycles. The attained measures of electrochemical performance indicate that PIZCON is an excellent supercapacitor electrode material.
Strain-Induced Tunable Band Offsets in Blue Phosphorus and WSe2 van der Waals Heterostructure
Authors: Zhang Lingxia , Huang Le , Yin Tao , Yang Yibin
Published: April 2021

The electronic structure and band offsets of blue phosphorus/WSe2 van der Waals (vdW) heterostructure are investigated via performing first-principles calculations. Blue phosphorus/WSe2 vdW heterostructure exhibits modulation of bandgaps by the applied vertical compressive strain, and a large compressive strain of more than 23% leads to a semiconductor-to-metal transition. Blue phosphorus/WSe2 vdW heterostructure is demonstrated to have a type-II band alignment, which promotes the spontaneous spatial separation of photo-excited electrons and holes. Furthermore, electrons concentrating in BlueP and holes in WSe2 can be enhanced by applied compressive strain, resulting in an increase of carrier concentration. Therefore, these properties make blue phosphorus/WSe2 vdW heterostructure a good candidate for future applications in photodetection.
Immune-Related Erythema Nodosum Mimicking in Transit Melanoma Metastasis on [18F]-FDG PET/CT
Authors: Seban Romain-David , Vermersch Camille , Champion Laurence , Bonsang Benjamin , Roger Anissa , Ghidaglia Jerome
Published: April 2021

Early detection of immune-related adverse events (irAEs) with immune checkpoint inhibitors (ICIs) is crucial, particularly when these are likely to mimic tumor progression, as well as sarcoid-like reactions. Here, we report the case of a 68-year woman, with a history of four primary cutaneous melanomas (thickest lesion with BRAF mutation removed from the left axilla 2 years before), who was diagnosed with BRAF V600E-mutant metastatic melanoma and treated by ICI targeting the PD-1 receptor. Follow-up whole-body positron emission tomography/computed tomography (PET/CT) using 18F-fluorodeoxyglucose ([18F]-FDG) was performed at 15 months, and FDG-avid subcutaneous nodules on her legs were detected. A biopsy from a lesion on her right leg was obtained, and histology strongly suggested erythema nodosum. Given the isolated nature of these lesions, the normal serum Angiotensin-Converting Enzyme and the context of ICI, an immune-related sarcoid-like reaction was retained as the most likely diagnosis. Recent literature in immune-oncology suggests that erythema nodosum could be directly related to ICI(s). Although erythema nodosum is a rare occurrence with imaging features overlapping with malignancy, it should be considered in the differential diagnosis of suspicious in-transit metastasis, especially when the patient is treated with ICIs and when lesions follow a bilateral distribution. In conclusion, nuclear medicine physicians should keep in mind this irAE when interpreting PET/CT scans in clinical practice in order to avoid false-positive findings.
The Transformative Role of Peer Learning Projects in 21st Century Schools—Achievements from Five Portuguese Educational Institutions
Authors: Carvalho Ana Raquel , Santos Carlos
Published: April 2021

Rethinking the role of education in the 21st century implies acknowledging the power of learning and the urgency of making learning provision more meaningful, inclusive, and student-centred, which assumes particular importance when learner disengagement is still a global issue in elementary and secondary education. Rooted in social constructivism principles, peer learning is a learner-centred approach that facilitates the development of soft and technical skills, with evidence-based contributions to learners’ academic performance under the cognitive, affective, and social dimensions. This study aims to find evidence of the transformative role of peer learning projects in four Portuguese secondary schools and a higher education institution through teachers and peer teacher students’ (PTS) perceptions of these projects’ purpose, implementation, and impact on the educational community, particularly on PTS. Data were collected by means of a semi-structured in-depth interview and a survey by questionnaire, and content analysis and descriptive statistics were the techniques used. Results show cooperation and interpersonal skills’ improvement as major strengths of these projects, whereas the challenges are mostly organisational, e.g., reduced teacher service time and coordination of learners’ schedules. Conclusions highlight the potential of peer learning projects to promote pedagogical transformation and innovation in 21st century schools.
Digital Addiction: Systematic Review of Computer Game Addiction Impact on Adolescent Physical Health
Authors: Aziz Norshakirah , Nordin Md Jan , Abdulkadir Said Jadid , Salih Muhammad Muhaimin M.
Published: April 2021

The advancement of technology has enabled powerful microprocessors to render high-quality graphics for computer gaming. Despite being intended for leisure purposes, several components of the games alongside the gamer’s environmental factors have resulted in digital addiction (DA) towards computer games such as massively multiplayer online games (MMOG). Excessive gaming among adolescents has various negative impacts on an individual. However, only a few researchers have addressed the impact of DA on physical health. Thus, the primary objective of this research is to study the impact of DA on physical health among Malaysian adolescents. This study focuses on Malaysian adolescents of ages 12–18 years old who are addicted to computer games, specifically the MMOG. The methodology used for the study involves focus group discussions (FGD) and extensive literature study. The FGD sessions have involved both medical experts and game experts. The outcome of FGD discussion is recorded and justified with the existing relevant literature from high-impact journals, theses, conferences, books, and reports. This paper provides evidence of five physical health impacts of DA associated with adolescents. The factors of DA, the addiction components existing in MMOG computer games, and the DA health risk assessment are presented in the results section. Based on the study, it is concluded that DA among Malaysian adolescents can cause various impacts on physical health such as obesity, back pain, and neck pain, orthopaedic/joint muscle, eyesight problem, hearing problem, as well as physical inactivity.
IPDCA: Intelligent Proficient Data Collection Approach for IoT-Enabled Wireless Sensor Networks in Smart Environments
Authors: Osamy Walid , Khedr Ahmed M. , El-Sawy Ahmed A. , Salim Ahmed , Vijayan Dilna
Published: April 2021

The Internet of Things (IoT) enables the interrelation of physical things and devices that can be accessed through the internet and it simply forms a single integrated network of various things. An IoT-facilitated smart city scenario spans several sectors, such as industrial applications, public transportation, smart grid, emergency services, health care, etc. In this paper, we propose an Intelligent Proficient Data Collection Approach (IPDCA) to deliver public data in a large-scale smart city set-up. IPDCA utilizes public vehicles as the mobile data collectors (D-collectors) that read (or collect) data from multiple Access Points (APs) and send them back to the central Base Station (BS). Moreover, IPDCA adopts a modified Bat algorithm for path finding of D-collectors, where we extend the Bat algorithm to solve our discrete optimization problem. Besides, for selecting D-collectors in smart city settings, we use a multi-objective fitness function that considers the count, travelled distance, and storage of D-collectors to ensure optimal use of resources. Efficiency of the proposed mechanism is proved through simulations.
Simultaneous Minimization of Energy Losses and Greenhouse Gas Emissions in AC Distribution Networks Using BESS
Authors: Molina-Martin Federico , Montoya Oscar Danilo , Grisales-Noreña Luis Fernando , Hernández Jesus C. , Ramírez-Vanegas Carlos A.
Published: April 2021

The problem of the optimal operation of battery energy storage systems (BESSs) in AC grids is addressed in this paper from the point of view of multi-objective optimization. A nonlinear programming (NLP) model is presented to minimize the total emissions of contaminant gasses to the atmosphere and costs of daily energy losses simultaneously, considering the AC grid complete model. The BESSs are modeled with their linear relation between the state-of-charge and the active power injection/absorption. The Pareto front for the multi-objective optimization NLP model is reached through the general algebraic modeling system, i.e., GAMS, implementing the pondered optimization approach using weighting factors for each objective function. Numerical results in the IEEE 33-bus and IEEE 69-node test feeders demonstrate the multi-objective nature of this optimization problem and the multiple possibilities that allow the grid operators to carry out an efficient operation of their distribution networks when BESS and renewable energy resources are introduced.
The Effect of Virtual Reality Rehabilitation on Balance in Patients with Parkinson’s Disease: A Systematic Review and Meta-Analysis
Authors: Wang Wenjing , Wong Sharon Sui-lam , Lai Frank Ho-yin
Published: April 2021

As a popular method, virtual reality (VR) is still controversial in its effect on the balance function of patients with Parkinson’s disease. This systematic review aims to discuss such effects of VR and to compare it with that resulting from traditional therapies. A comprehensive search was conducted for randomized controlled trials published from 2000 to 2020 through the following databases: PubMed, Web of Science, CINAHL, Embase, Cochrane Library. Fifteen articles were included for the systematic review. An evaluation on their methodological qualities was performed using the PEDro scale, followed by an assessment of their risk of biases in accordance with the Cochrane Handbook for Systematic Reviews of Interventions for quality assessment. In terms of dynamic balance, the BBS score of the VR group was significantly improved when compared with the control group (SMD = 0.52, 95% CI = 0.31–0.73). However, no significant difference was observed between the two groups on TUG (SMD = −0.26; 95% CI = −0.62–0.1; p = 0.16). Besides, the VR group also showed better results in improving patients’ static balance, balance confidence, and quality of life. A funnel plot was created to investigate the effects of each study included in the meta-analysis in order to identify any existing publication bias. This systematic review shows that the application of VR leads to more significant improvement in the balance of patients with Parkinson’s disease than having them perform traditional exercises. It can be used as an auxiliary method of rehabilitation.
The Issues of the Air-Fuel Ratio in Exhaust Emissions Tests Carried out on a Chassis Dynamometer
Authors: Gis Wojciech , Taubert Sławomir
Published: April 2021

Vehicle exhaust emission tests use exhaust sampling systems that dilute the exhaust gas with ambient air. The dilution factor DF is calculated assuming that the combustion is complete, and that the engine is operated at a stoichiometric air-fuel ratio (AFR). These assumptions are not always met. This is especially true for diesel engines. This article discusses the tests to find out what the average lambda (λ) over the ARTEMIS, WLTC and NEDC driving cycles is and how this affects the result of the emission measurements. Measurements were carried out on a chassis dynamometer equipped with a standard emission measurement system used during the homologation. The λ was calculated using the Brettschneider equation. The dilution ratio DR was also determined by measuring the CO2 concentration in the raw exhaust gas. The CO2-tracer method used for this was modified. The median of the λ for a CI vehicle was 1.23–3.31, which makes the relative percentage difference between the DF and DR (ΔDF) in the range of 28–167%. For a SI vehicle homologated under the WLTP procedure, the median of the λ for the WLTC and ARTEMIS cycles was close to one and ΔDF for most cycles does not exceed 10%. In order to reduce the influence of the error of DF determination on the result of the emission measurement, it is recommended to use exhaust gas sampling systems that allow to determine the actual dilution ratio or to use the lowest possible dilution. The PAS-CVS system seems to be the most promising.
Implementing Radical Innovation in Renewable Energy Experience Curves
Authors: Kerr Paul , Noble Donald R. , Hodges Jonathan , Jeffrey Henry
Published: April 2021

Cost reductions in nascent forms of Renewable Energy Technology (RET) are essential for them to contribute to the energy mix. Policy intervention can facilitate this cost reduction; however, this may require a significant investment from the public sector. These cost reductions fall into two broad categories: (1) incremental cost reductions through continual improvements to existing technologies, and (2) radical innovation where technologies that significantly differ from the incumbents are developed. This study presents a modelling methodology to integrate radical innovation in RET experience curve and learning investment analysis, using wave energy as an example nascent RET. This aims to quantify the potential effects of radical innovation on the learning investment, allowing the value of successful innovation to be better analysed. The study highlights the value offered by radical innovations in long-term deployment scenarios for wave energy. This suggests that high-risk R&D efforts in nascent RET sectors, even with low success rates, could still present significant expected value in offsetting future revenue support.
A Wirelessly Rechargeable AA Battery Using Electrodynamic Wireless Power Transmission
Authors: Smith Spencer E. , Halim Miah A. , Chyczewski Stasiu T. , Rendon-Hernandez Adrian A. , Arnold David P.
Published: April 2021

We report the design, fabrication, and characterization of a prototype that meets the form, fit, and function of a household 1.5 V AA battery, but which can be wirelessly recharged without removal from the host device. The prototype system comprises a low-frequency electrodynamic wireless power transmission (EWPT) receiver, a lithium polymer energy storage cell, and a power management circuit (PMC), all contained within a 3D-printed package. The EWPT receiver and overall system are experimentally characterized using a 238 Hz sinusoidal magnetic charging field and either a 1000 µF electrolytic capacitor or a lithium polymer (LiPo) cell as the storage cell. The system demonstrates a minimal operating field as low as 50 µTrms (similar in magnitude to Earth’s magnetic field). At this minimum charging field, the prototype transfers a maximum dc current of 50 µA to the capacitor, corresponding to a power delivery of 118 µW. The power effectiveness of the power management system is approximately 49%; with power effectiveness defined as the ratio between actual output power and the maximum possible power the EWPT receiver can transfer to a pure resistive load at a given field strength.
Towards the Elucidation of Assimilative nasABC Operon Transcriptional Regulation in Haloferax mediterranei
Authors: Pastor-Soler Sandra , Camacho Mónica , Bautista Vanesa , Bonete María-José , Esclapez Julia
Published: April 2021

The assimilatory pathway of the nitrogen cycle in the haloarchaeon Haloferax mediterranei has been well described and characterized in previous studies. However, the regulatory mechanisms involved in the gene expression of this pathway remain unknown in haloarchaea. This work focuses on elucidating the regulation at the transcriptional level of the assimilative nasABC operon (HFX_2002 to HFX_2004) through different approaches. Characterization of its promoter region using β-galactosidase as a reporter gene and site-directed mutagenesis has allowed us to identify possible candidate binding regions for a transcriptional factor. The identification of a potential transcriptional regulator related to nitrogen metabolism has become a real challenge due to the lack of information on haloarchaea. The investigation of protein–DNA binding by streptavidin bead pull-down analysis combined with mass spectrometry resulted in the in vitro identification of a transcriptional regulator belonging to the Lrp/AsnC family, which binds to the nasABC operon promoter (p.nasABC). To our knowledge, this study is the first report to suggest the AsnC transcriptional regulator as a powerful candidate to play a regulatory role in nasABC gene expression in Hfx. mediterranei and, in general, in the assimilatory nitrogen pathway.
Associations between Physical Activity, Sunshine Duration and Osteoporosis According to Obesity and Other Lifestyle Factors: A Nested Case–Control Study
Authors: Min Chan-Yang , Yoo Dae-Myoung , Choi Hyo-Geun
Published: April 2021

(1) Background: The purpose of the study was to evaluate the associations between physical activity (PA), sunshine duration (SD) and the occurrence of osteoporosis according to lifestyle status. (2) Methods: Data from the Korean National Health Insurance Service–National Sample Cohort (NHIS-NSC) collected from 2009 to 2015 were used. Osteoporosis (n = 19,351) and control (n = 38,702) participants were matched in a 1:2 ratio according to age, sex, income, and region of residence. PA was classified as moderate- to high-intensity PA (MHPA) or low-intensity PA (LPA) based on the International Physical Activity Questionnaire (IPAQ). SD was classified as short (≤6 h) or long (>6 h). Conditional logistic regression was used to calculate the odds ratios (ORs) with 95% confidence intervals (CIs) of MHPA and long SD for the occurrence of osteoporosis. Subgroup analyses were performed according to SD (or PA), obesity, smoking, and alcohol consumption. (3) The adjusted OR of MHPA for osteoporosis was 0.90 (95% CI = 0.87–0.94). The results were consistent in the age/sex, SD, obesity, smoking, and alcohol consumption subgroups, but not the <60-year-old male and underweight subgroups. The adjusted OR of long SD for osteoporosis was 0.96 (95% CI = 0.93–1.00). The findings were consistent in the <60-year-old female, obese, nonsmoker, and <1 time a week alcohol consumption subgroups. (4) Conclusions: We suggest that both higher intensity of PA and long SD could decrease the risk of osteoporosis. Specifically, PA could decrease the risk of osteoporosis in individuals with most characteristics except male sex or underweight. Long SD could decrease the risk of osteoporosis in young females, obese individuals, nonsmokers, and individuals with lower alcohol consumption.
Association between Training Load and Well-Being Measures in Young Soccer Players during a Season
Authors: Nobari Hadi , Alves Ana Ruivo , Haghighi Hamed , Clemente Filipe Manuel , Carlos-Vivas Jorge , Pérez-Gómez Jorge , Ardigò Luca Paolo
Published: April 2021

This study aimed to analyze the correlations among weekly (w) acute workload (wAW), chronic workload (wCW), acute/chronic workload ratio (wACWR), training monotony (wTM), training strain (wTS), sleep quality (wSleep), delayed onset muscle soreness (wDOMS), fatigue (wFatigue), stress (wStress), and Hooper index (wHI) in pre-, early, mid-, and end-of-season. Twenty-one elite soccer players (age: 16.1 ± 0.2 years) were monitored weekly on training load and well-being for 36 weeks. Higher variability in wAW (39.2%), wFatigue (84.4%), wStress (174.3%), and wHI (76.3%) at the end-of-season were reported. At mid-season, higher variations in wSleep (59.8%), TM (57.6%), and TS (111.1%) were observed. Moderate to very large correlations wAW with wDOMS (r = 0.617, p = 0.007), wFatigue, wStress, and wHI were presented. Similarly, wCW reported a meaningful large association with wDOMS (r = 0.526, p < 0.001); moderate to very large associations with wFatigue (r = 0.649, p = 0.005), wStress, and wHI. Moreover, wTM presented a large correlation with wSleep (r = 0.515, p < 0.001); and a negatively small association with wStress (r = −0.426, p = 0.003). wTS showed a small to large correlation with wSleep (r = 0.400, p = 0.005) and wHI; also, a large correlation with wDOMS (r = 0.556, p = 0.028) and a moderate correlation with wFatigue (r = 0.343, p = 0.017). Wellness status may be considered a useful tool to provide determinant elite players’ information to coaches and to identify important variations in training responses.
Wastewater Surveillance for SARS-CoV-2 on College Campuses: Initial Efforts, Lessons Learned, and Research Needs
Authors: Harris-Lovett Sasha , Nelson Kara L. , Beamer Paloma , Bischel Heather N. , Bivins Aaron , Bruder Andrea , Butler Caitlyn , Camenisch Todd D. , De Long Susan K. , Karthikeyan Smruthi , Larsen David A. , Meierdiercks Katherine , Mouser Paula J. , Pagsuyoin Sheree , Prasek Sarah M. , Radniecki Tyler S. , Ram Jeffrey L. , Roper D. Keith , Safford Hannah , Sherchan Samendra P. , Shuster William , Stalder Thibault , Wheeler Robert T. , Korfmacher Katrina Smith
Published: April 2021

Wastewater surveillance for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging approach to help identify the risk of a coronavirus disease (COVID-19) outbreak. This tool can contribute to public health surveillance at both community (wastewater treatment system) and institutional (e.g., colleges, prisons, and nursing homes) scales. This paper explores the successes, challenges, and lessons learned from initial wastewater surveillance efforts at colleges and university systems to inform future research, development and implementation. We present the experiences of 25 college and university systems in the United States that monitored campus wastewater for SARS-CoV-2 during the fall 2020 academic period. We describe the broad range of approaches, findings, resources, and impacts from these initial efforts. These institutions range in size, social and political geographies, and include both public and private institutions. Our analysis suggests that wastewater monitoring at colleges requires consideration of local information needs, sewage infrastructure, resources for sampling and analysis, college and community dynamics, approaches to interpretation and communication of results, and follow-up actions. Most colleges reported that a learning process of experimentation, evaluation, and adaptation was key to progress. This process requires ongoing collaboration among diverse stakeholders including decision-makers, researchers, faculty, facilities staff, students, and community members.
Sella Turcica Morphology on Cephalometric Radiographs and Dental Abnormalities—Is There Any Association?—Systematic Review
Authors: Jankowski Tomasz , Jedliński Maciej , Grocholewicz Katarzyna , Janiszewska-Olszowska Joanna
Published: April 2021

Background: The sella turcica is a saddle-like structure in the middle cranial fossa on the intracranial surface of the sphenoid bone, visible on lateral cephalograms routinely conducted for orthodontic diagnosis. The development of facial structures follows similar traits to the sella turcica: glandular anomalies may be associated with functional disorders, e.g., altered hormonal levels, thus influencing dental development. The aim of this study is to find out if there is any association between the morphology of the sella turcica on cephalometric radiographs and the presence of dental abnormalities. (2) Methods: The search was conducted on 27 January 2021 in four search engines: Medline (PubMed Central), Scopus, Web of Science, Embase. The keywords used in the search strategy were as follows: “sella turcica” AND (“dental abnormalities” OR “dental anomalies” OR “malocclusion”). Since all the studies finally included were retrospective case–control studies, the Newcastle–Ottawa Quality Assessment Form for Case–Control Studies was applied. (3) Results: The search strategy identified 465 articles: 289 from PubMed, 121 from Scopus, 32 from Web of Science and 23 from Embase. Finally, 10 full-text papers were included into qualitative analysis. (4) Conclusions: Sella turcica bridging is very frequent among orthodontic patients. A clear association exists between dental abnormalities and sella turcica bridging.
Choosing the Right Partner for Medication Related Osteonecrosis of the Jaw: What Central European Dentists Know
Authors: Bruckmoser Emanuel , Palaoro Miriam , Latzko Lukas , Schnabl Dagmar , Neururer Sabrina B. , Laimer Johannes
Published: April 2021

Medication-related osteonecrosis of the jaw (MRONJ) is a side effect of antiresorptive drugs. In this online survey, the awareness and knowledge of dentists regarding MRONJ was evaluated, and potential implications for oncologists are discussed. Questionnaires were emailed to dentists from Germany, Austria, Switzerland, and South Tyrol to evaluate disease-related knowledge and management. In addition to the overall score, a separate score was calculated for knowledge (maximum score: 15 points) and management (maximum score: 6 points) questions, and 1197 valid replies with completed questionnaires were received. The mean overall score was 10.45 ± 3.97 points, the mean knowledge score was 7.68 ± 3.05 points, and the mean management score was 2.76 ± 1.77 points. Factors influencing the outcome of the overall score were age, specialization, continuous professional education, and the number of dental screening exams in patients before antiresorptive therapy. Due to the considerable lack of knowledge regarding MRONJ among dentists, MRONJ patients and subjects at risk should be guided towards specialists for dental screening, treatment, and follow-up. This is important from an oncologic point of view to avoid any delay for treatment start of antiresorptives, and to reveal a potentially emerging osteonecrosis at an early stage, thus, avoiding the need for interruption or even cancellation of antiresorptive therapy.
The Effect of Containment Measures during the Covid-19 Pandemic to Sedentary Behavior of Thai Adults: Evidence from Thailand’s Surveillance on Physical Activity 2019–2020
Authors: Katewongsa Piyawat , Potharin Danusorn , Rasri Niramon , Palakai Rungrat , Widyastari Dyah Anantalia
Published: April 2021

Measures to contain the spread of coronavirus disease 2019 (Covid-19) imposed by governments have undoubtedly impacted on preventing its spread but may have also produced longer periods of sedentary living across all segments of society. To examine this phenomenon, this study compared the sedentary behavior (SB) of Thai adults before and during the Covid-19 pandemic. The 2019 and 2020 datasets of Thailand’s Surveillance on Physical Activity (SPA) were employed. A total of 5379 (SPA2019) and 6531 (SPA202020) persons age 18–64 years who had access to the Internet were included in the analysis. Measures imposed to contain the spread of Covid-19 infection were significantly associated with lower opportunity of Thai adults for work-related physical movement, and that increased their SB, particularly with the shift from onsite to online working platforms. Cumulative SB increased from 824 (before the pandemic) to 875 min/day during the pandemic. The odds of accumulating >13 h/day of SB was highest among females, young adults, those who completed post-secondary education, unemployed or working in the non-agriculture sector, having a chronic disease/condition, residing in an urban area, and living in a ‘higher-risk’ pandemic zone. The insignificant association of physical activity (PA) and the Fit from Home (FFH) intervention in reducing SB during the pandemic suggests that PA is not directly associated with SB, and that the FFH intervention was insufficient to prevent SB.
Does COVID-19 Affect the Behavior of Buying Fresh Food? Evidence from Wuhan, China
Authors: Chen Jing , Zhang Yong , Zhu Shiyao , Liu Lei
Published: April 2021

COVID-19 first appeared in Wuhan city of Hubei Province in China in December 2019. It has a substantial impact on human life all around the world, especially for citizens. The threat of COVID-19 has resulted in people shopping online to get fresh food and reduce outdoor trips. Collecting data from adult internet users in Wuhan, China in 2020, this study aims to explore the influence of COVID-19 on fresh food shopping behavior. In addition, a comparison and ordered logit model are constructed to demonstrate the changes and effects of COVID-19. The results suggest that more citizens in Wuhan city will buy fresh food online and the cost and frequency are also increased. The experience of online shopping for fresh food during the lock-down days has promoted more online shopping. The factors, such as frequency of online shopping before the COVID-19 outbreak, frequency of online shopping during the COVID-19 pandemic, and age, have a negative effect on the proportion of online shopping after the lock-down days, while the proportion of online shopping before the COVID-19 outbreak, the proportion of online shopping during the COVID-19 pandemic, and travel time of in-store shopping before the COVID-19 outbreak have a positive effect. The results provide insights for managers, city planners, and policymakers.
The Spatial Interaction Effect of Environmental Regulation on Urban Innovation Capacity: Empirical Evidence from China
Authors: Zhou Ruomeng , Zhang Yunsheng , Gao Xincai
Published: April 2021

This paper applies a spatial econometric model to measure the impact of environmental regulation on urban innovation capacity from a spatial interaction perspective by using panel data from 41 cities in the Yangtze River Delta urban agglomeration from 2009 to 2018. The study findings are as follows: first, environmental regulation has a significant positive impact on urban innovation capacity and a significant positive spatial spillover effect; second, innovation capacity has significant positive spatial dependence; third, city informatization level, government expenditures on science and technology, city economic scale, and industrial development level all positively affect the innovation capacity of neighboring cities and all have positive spatial spillover effects on the innovation capacity of neighboring cities; and finally, city expansion reduces the innovation capacity of a city and has negative spatial spillover effects on the innovation capacity of neighboring cities.
Regulatory Light Chains in Cardiac Development and Disease
Authors: Markandran Kasturi , Poh Jane Wenjin , Ferenczi Michael A. , Cheung Christine
Published: April 2021

The role of regulatory light chains (RLCs) in cardiac muscle function has been elucidated progressively over the past decade. The RLCs are among the earliest expressed markers during cardiogenesis and persist through adulthood. Failing hearts have shown reduced RLC phosphorylation levels and that restoring baseline levels of RLC phosphorylation is necessary for generating optimal force of muscle contraction. The signalling mechanisms triggering changes in RLC phosphorylation levels during disease progression remain elusive. Uncovering this information may provide insights for better management of heart failure patients. Given the cardiac chamber-specific expression of RLC isoforms, ventricular RLCs have facilitated the identification of mature ventricular cardiomyocytes, opening up possibilities of regenerative medicine. This review consolidates the standing of RLCs in cardiac development and disease and highlights knowledge gaps and potential therapeutic advancements in targeting RLCs.
Different Approaches in Therapy Aiming to Stabilize an Unstable Atherosclerotic Plaque
Authors: Kowara Michal , Cudnoch-Jedrzejewska Agnieszka
Published: April 2021

Atherosclerotic plaque vulnerability is a vital clinical problem as vulnerable plaques tend to rupture, which results in atherosclerosis complications—myocardial infarctions and subsequent cardiovascular deaths. Therefore, methods aiming to stabilize such plaques are in great demand. In this brief review, the idea of atherosclerotic plaque stabilization and five main approaches—towards the regulation of metabolism, macrophages and cellular death, inflammation, reactive oxygen species, and extracellular matrix remodeling have been presented. Moreover, apart from classical approaches (targeted at the general mechanisms of plaque destabilization), there are also alternative approaches targeted either at certain plaques which have just become vulnerable or targeted at the minimization of the consequences of atherosclerotic plaque erosion or rupture. These alternative approaches have also been briefly mentioned in this review.
Ubiquitin and Ubiquitin-Like Proteins and Domains in Ribosome Production and Function: Chance or Necessity?
Authors: Martín-Villanueva Sara , Gutiérrez Gabriel , Kressler Dieter , de la Cruz Jesús
Published: April 2021

Ubiquitin is a small protein that is highly conserved throughout eukaryotes. It operates as a reversible post-translational modifier through a process known as ubiquitination, which involves the addition of one or several ubiquitin moieties to a substrate protein. These modifications mark proteins for proteasome-dependent degradation or alter their localization or activity in a variety of cellular processes. In most eukaryotes, ubiquitin is generated by the proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor, or as a single N-terminal moiety to ribosomal proteins, which are practically invariably eL40 and eS31. Herein, we summarize the contribution of the ubiquitin moiety within precursors of ribosomal proteins to ribosome biogenesis and function and discuss the biological relevance of having maintained the explicit fusion to eL40 and eS31 during evolution. There are other ubiquitin-like proteins, which also work as post-translational modifiers, among them the small ubiquitin-like modifier (SUMO). Both ubiquitin and SUMO are able to modify ribosome assembly factors and ribosomal proteins to regulate ribosome biogenesis and function. Strikingly, ubiquitin-like domains are also found within two ribosome assembly factors; hence, the functional role of these proteins will also be highlighted.
The Role of Resveratrol Administration in Human Obesity
Authors: Mongioì Laura M. , La Vignera Sandro , Cannarella Rossella , Cimino Laura , Compagnone Michele , Condorelli Rosita A. , Calogero Aldo E.
Published: April 2021

Obesity is a widespread disease that is associated with numerous and serious comorbidities. These include metabolic syndrome, diabetes mellitus, cardiovascular-cerebrovascular disease, hypertension, obstructive sleep apnea syndrome, cancer, and sexual and hormonal disorders. The treatment of obesity has therefore become a goal of great clinical and social relevance. Among the therapeutic strategies against obesity, resveratrol has aroused great interest. This polyphenol has anticancer and antioxidant properties and cytoprotective and anti-inflammatory effects. Other favorable effects attributed to resveratrol are anti-lipid, anti-aging, anti-bacterial, anti-viral, and neuroprotective actions. Administration of resveratrol appears to improve the metabolic profile in obese and/or insulin-resistant patients. This article aims to review the main results of clinical studies evaluating the effects of administering resveratrol alone in overweight/obese patients.
Characterization of Long Non-Coding RNAs in Systemic Sclerosis Monocytes: A Potential Role for PSMB8-AS1 in Altered Cytokine Secretion
Authors: Servaas Nila H. , Mariotti Barbara , van der Kroef Maarten , Wichers Catharina G. K. , Pandit Aridaman , Bazzoni Flavia , Radstake Timothy R. D. J. , Rossato Marzia
Published: April 2021

Systemic sclerosis (SSc) is a chronic autoimmune disease mainly affecting the connective tissue. In SSc patients, monocytes are increased in circulation, infiltrate affected tissues, and show a pro-inflammatory activation status, including the so-called interferon (IFN) signature. We previously demonstrated that the dysregulation of the IFN response in SSc monocytes is sustained by altered epigenetic factors as well as by upregulation of the long non-coding RNA (lncRNA) NRIR. Considering the enormously diverse molecular functions of lncRNAs in immune regulation, the present study investigated the genome-wide profile of lncRNAs in SSc monocytes, with the aim to further unravel their possible role in monocyte dysregulation and disease pathogenesis. Transcriptomic data from two independent cohorts of SSc patients identified 886 lncRNAs with an altered expression in SSc monocytes. Differentially expressed lncRNAs were correlated with neighboring protein coding genes implicated in the regulation of IFN responses and apoptotic signaling in SSc monocytes. In parallel, gene co-expression network analysis identified the lncRNA PSMB8-AS1 as a top-ranking hub gene in co-expression modules implicated in cell activation and response to viral and external stimuli. Functional characterization of PSMB8-AS1 in monocytes demonstrated that this lncRNA is involved in the secretion of IL-6 and TNFα, two pivotal pro-inflammatory cytokines altered in the circulation of SSc patients and associated with fibrosis and disease severity. Collectively, our data showed that lncRNAs are linked to monocyte dysregulation in SSc, and highlight their potential contribution to disease pathogenesis.
Correction: Paszkiewicz et al. A Peripheral CB1R Antagonist Increases Lipolysis, Oxygen Consumption Rate, and Markers of Beiging in 3T3-L1 Adipocytes Similar to RIM, Suggesting That Central Effects Can Be Avoided. Int. J. Mol. Sci. 2020, 21, 6639
Authors: Paszkiewicz Rebecca L. , Bergman Richard N. , Santos Roberta S. , Frank Aaron P. , Woolcott Orison O. , Iyer Malini S. , Stefanovski Darko , Clegg Deborah J. , Kabir Morvarid
Published: April 2021

The authors wish to make the following corrections to this paper [...]
Plasminogen Activators in Neurovascular and Neurodegenerative Disorders
Authors: Yepes Manuel , Woo Yena , Martin-Jimenez Cynthia
Published: April 2021

The neurovascular unit (NVU) is a dynamic structure assembled by endothelial cells surrounded by a basement membrane, pericytes, astrocytes, microglia and neurons. A carefully coordinated interplay between these cellular and non-cellular components is required to maintain normal neuronal function, and in line with these observations, a growing body of evidence has linked NVU dysfunction to neurodegeneration. Plasminogen activators catalyze the conversion of the zymogen plasminogen into the two-chain protease plasmin, which in turn triggers a plethora of physiological events including wound healing, angiogenesis, cell migration and inflammation. The last four decades of research have revealed that the two mammalian plasminogen activators, tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), are pivotal regulators of NVU function during physiological and pathological conditions. Here, we will review the most relevant data on their expression and function in the NVU and their role in neurovascular and neurodegenerative disorders.
The Effects of Atorvastatin on Global Cerebral Ischemia-Induced Neuronal Death
Authors: Kho A Ra , Hong Dae Ki , Kang Beom Seok , Park Woo-Jung , Choi Kyung Chan , Park Kyoung-Ha , Suh Sang Won
Published: April 2021

(1) Background and Purpose: Global cerebral ischemia-induced severe hypoxic brain damage is one of the main causes of mortality and long-term neurologic disability even after receiving early blood reperfusion. This study aimed to test the hypothesis that atorvastatin potentially has neuroprotective effects in global cerebral ischemia (GCI). (2) Methods: We performed two sets of experiments, analyzing acute (1-week) and chronic (4-week) treatments. For the vehicle (Veh) and statin treatments, 1 mL of 0.9% saline and 5 mg/kg of atorvastatin (ATOR) were administered orally. For histological analysis, we used the following staining protocols: Fluoro-Jade B and NeuN, 4-hydroxynonenal, CD11b and GFAP, IgG, SMI71, and vWF. Finally, we evaluated the cognitive function with a battery of behavioral tests. (3) Results: The GCI-ATOR group showed significantly reduced neuronal death, oxidative stress, inflammation, and BBB disruption compared with the GCI-Veh group. Moreover, the GCI-ATOR group showed decreased endothelial damage and VV proliferation and had significantly improved cognitive function compared with the GCI-Veh group in both models. (4) Conclusions: ATOR has neuroprotective effects and helps recover the cognitive function after GCI in rats. Therefore, administration of atorvastatin may be a therapeutic option in managing GCI after CA.
Inhibition of Glycolysis Suppresses Cell Proliferation and Tumor Progression In Vivo: Perspectives for Chronotherapy
Authors: Horváthová Jana , Moravčík Roman , Matúšková Miroslava , Šišovský Vladimír , Boháč Andrej , Zeman Michal
Published: April 2021

A high rate of glycolysis is considered a hallmark of tumor progression and is caused by overexpression of the enzyme 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3). Therefore, we analyzed the possibility of inhibiting tumor and endothelial cell metabolism through the inhibition of PFKFB3 by a small molecule, (E)-1-(pyridin-4-yl)-3-(quinolin-2-yl)prop-2-en-1-one (PFK15), as a promising therapy. The effects of PFK15 on cell proliferation and apoptosis were analyzed on human umbilical vein endothelial cells (HUVEC) and the human colorectal adenocarcinoma cell line DLD1 through cytotoxicity and proliferation assays, flow cytometry, and western blotting. The results showed that PFK15 inhibited the proliferation of both cell types and induced apoptosis with decreasing the Bcl-2/Bax ratio. On the basis of the results obtained from in vitro experiments, we performed a study on immunodeficient mice implanted with DLD1 cells. We found a reduced tumor mass after morning PFK15 treatment but not after evening treatment, suggesting circadian control of underlying processes. The reduction in tumor size was related to decreased expression of Ki-67, a marker of cell proliferation. We conclude that inhibition of glycolysis can represent a promising therapeutic strategy for cancer treatment and its efficiency is circadian dependent.
Lasius fuliginosus Nest Carton as a Source of New Promising Bioactive Extracts with Chemopreventive Potential
Authors: Wagner Grzegorz Karol , Jaszek Magdalena , Staniec Bernard , Prendecka Monika , Pigoń Dominika , Belcarz Anna , Stefaniuk Dawid , Matuszewska Anna , Pietrykowska-Tudruj Ewa , Zagaja Mirosław
Published: April 2021

Six new water extracts (E1–E6) were obtained from nest carton produced by jet black ants Lasius fuliginosus and tested for their biochemical and bioactive properties, including antioxidative and anticancer effects. The present study demonstrated significant qualitative and quantitative differences in the content of individual biochemical constituents, as well as bioactive properties between the investigated samples. All tested extracts demonstrated antioxidant properties (determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods), and the highest antioxidative potential was recorded in extracts E1 and E2 (188.96 and 313.67 μg/mL of ascorbic acid equivalent for ABTS and 176.42 and 202.66 μg/mL for DPPH reagent). Furthermore the six extracts exhibited strong inhibitory activity towards human melanoma cells of the A-375 CRL-1619 line in a dose-dependent manner. The most interesting chemopreventive activity was exhibited by extract E2, which inhibited the proliferation of A-375 cells to the greatest extent, while having a minimal effect on Vero cells. The effect on cancer cells has been confirmed using the Electric Cell-substrate Impedance Sensing (ECIS) technique. Significant impedance changes have been detected in A-375 and Vero cells following the administration of extract E2. The obtained results are really promising and constitute the basis for further research on the nest carton of jet black ant.
Phosphatidylserine Supplementation as a Novel Strategy for Reducing Myocardial Infarct Size and Preventing Adverse Left Ventricular Remodeling
Authors: Schumacher David , Curaj Adelina , Staudt Mareike , Cordes Franziska , Dumitraşcu Andreea R. , Rolles Benjamin , Beckers Christian , Soppert Josefin , Rusu Mihaela , Simsekyilmaz Sakine , Kneizeh Kinan , Ramachandra Chrishan J. A. , Hausenloy Derek J. , Liehn Elisa A.
Published: April 2021

Phosphatidylserines are known to sustain skeletal muscle activity during intense activity or hypoxic conditions, as well as preserve neurocognitive function in older patients. Our previous studies pointed out a potential cardioprotective role of phosphatidylserine in heart ischemia. Therefore, we investigated the effects of phosphatidylserine oral supplementation in a mouse model of acute myocardial infarction (AMI). We found out that phosphatidylserine increases, significantly, the cardiomyocyte survival by 50% in an acute model of myocardial ischemia-reperfusion. Similar, phosphatidylserine reduced significantly the infarcted size by 30% and improved heart function by 25% in a chronic model of AMI. The main responsible mechanism seems to be up-regulation of protein kinase C epsilon (PKC-ε), the main player of cardio-protection during pre-conditioning. Interestingly, if the phosphatidylserine supplementation is started before induction of AMI, but not after, it selectively inhibits neutrophil’s activation, such as Interleukin 1 beta (IL-1β) expression, without affecting the healing and fibrosis. Thus, phosphatidylserine supplementation may represent a simple way to activate a pre-conditioning mechanism and may be a promising novel strategy to reduce infarct size following AMI and to prevent myocardial injury during myocardial infarction or cardiac surgery. Due to the minimal adverse effects, further investigation in large animals or in human are soon possible to establish the exact role of phosphatidylserine in cardiac diseases.