bluuebunny/crossref_metadata_2025 huggingface dataset:
Interrelation of growth/differentiation factor-15 level with laboratory and clinical and functional parameters of patients with coronary artery disease
Authors: Zakharyan E. A.
Published: June 2023

Growth differentiation factor 15 (GDF-15) is a circulating protein that is associated with various pathological conditions.

Aim. To study the relationship between the level of GDF-15 and labo­ratory, clinical and functional parameters of patients with coronary artery disease (CAD).

Material and methods. All patients with coronary artery disease under­went a general clinical examination, coronary angiography, extrac­ranial artery ultrasound examination, echocardiography, deter­mi­nation of the serum level of GDF-15 (ng/ml) and matrix metal­lopro­teinase 9 (ng/ml). Statistical processing of the obtained results was carried out using the Statistica 10.0 software.

Results. The study included 88 people (55 men and 33 women), 78 of whom were patients with an established diagnosis of CAD and 10 were healthy volunteers (control group). In the group of CAD patients, the median level of GDF-15 was 4,98 ng/ml [3,15; 8,24], while in the cont­rol group — 1,95 ng/ml [0,01; 2,45]. An association of GDF-15 con­centration with the age of patients was found (r=0,44; p<0,001). There was a direct correlation between the level of GDF-15 and interventricular septal thickness (r=0,33; p<0,05), heart failure stage (Strazhesko-Vasilenko classification) (r=0,23; p<0,05), the concentration of matrix metal­loproteinase 9 (r=0,24; p<0,05), carotid intima-media thickness (r=0,28; p<0,05), the number of coronary arteries that required revas­cularization during coronary artery bypass grafting (r=0,52; p<0,05), and multifocal atherosclerosis (r=0,23; p<0,05). In patients with athero­sclerotic lesions in 2 or 3 vascular beds, the level of GDF-15 was signifi­cantly higher than in patients with CAD (p<0,05).

Conclusion. The results obtained demonstrate the value of GDF-15 as a potential marker of atherosclerotic vascular changes, the severity of myocardial hypertrophy, and the severity of heart failure.
Cardiac resynchronization therapy: potential for arrhythmic risk modification
Authors: Ilov N. N. , Boitsov S. A. , Krivosheev Yu. S. , Nechepurenko A. A.
Published: June 2023

Aim. To evaluate the impact of cardiac resynchronization therapy (CRT) on the risk of sustained ventricular tachyarrhythmias (VT) in patients with heart failure (HF) with a implantable cardioverter-defibrillator (ICD) for the primary prevention of sudden cardiac death.

Material and methods. This single-center prospective clinical study included 470 patients (men, 84%) with HF at the age of 57 (51-62) years with a left ventricular ejection fraction (LVEF) of 29 (25-33)%. There were following exclusion criteria: indications for cardiac surgery, known channelopathies, previously registered VT. Depending on intraventricular conduction disorders, dual-chamber ICDs (42%) or CRT-D (58%) were implanted. After ICD implantation, patients were followed up for 24 months to register the end point — a first-time sustained paroxysm of VT detected by the ICD. A positive response to CRT was established in the case of an increase in LVEF by ≥5% of the initial level.

Results. A total of 388 patients underwent full postoperative follow-up. The studied arrhythmic endpoint occurred on average 21 (0,6) months after implantation in 104 patients (27%) with higher frequency in the ICD group. However, the differences in the VT rate in the study groups were statistically unreliable (30% in the ICD group versus 24% in the CRT-D group, p=0,142). It was found that the VT rate was reliably lower in CRT-responders (118 patients, 53%): 15% compared to 32% in the group of inefficient CRT. A 5% increase in the LFEF reduced the probability of VT occurrence by 3 times (odds ratio [OR]=0,34; 95% of CI: 0,13-0,86; p=002). Significant modification of arrhythmic risk was verified by increasing LFEF to 36-40% (OR=0,72; 95% CI: 0,63-0,82; p=0,04).

Conclusion. The results obtained indicate that effective CRT has the potential to modify arrhythmic risk in patients with HF, especially with an increase in LVEF to the level of 36-40%.
Mortality rates of acute types of coronary artery disease and sudden cardiac death in the Russian regions in 2019-2021
Authors: Samorodskaya I. V. , Bubnova M. G. , Akulova O. A. , Ostroushko N. I. , Drapkina O. M.
Published: June 2023

Aim. To compare standardized mortality rates (SMR) from acute types of coronary artery disease (CAD) and sudden cardiac death (SCD) with an assessment of their regional variability in the Russian Federation in 2019-2021.

Material and methods. Rosstat data on the average annual population and the number of deaths in one-year age groups for 2019-2021 in 82 Russian subjects from acute types of CAD and SCD were analyzed using following ICD-10 codes: I21.0-9 (acute primary) myocardial in­­farction (MI), I22.0-9 (recurrent MI), I20, I24.1-9 (other forms of acute coronary artery disease), I46.1 (SCD). SMR was calculated based on the European standard per 100,000 population. Average regional SMR values (M±SD), coefficients of variation (СV), nonparametric Wilcoxon T-test with Bonferroni adjustment were calculated (differences were considered significant at р<0,05).

Results. The average regional values of SMR from the sum of causes (acute types of CAD and SCD for 2019, 2020 and 2021) did not change significantly and amounted to 52,3±33,72; 53,84±36,17; 53,58±34,55, respectively. The annual increase in the average regional SMR for this period was noted from acute MI — 23,59±11,01; 24,85±10,92; 25,73±11,38, and other OF CAD — 19,47±26,17, 19,78±27,45; 20,54±26,79, respectively, in 2019, 2020 and 2021. A significant decrease in SMR occurred only from recurrent MI: from 7,19±5,38 to 6,97±5,97 in 2019-2020 (p=0,048) and to 4,99±4,25 in 2021 (p=0,023 to 2020). In 18 constituent entities of the Russian Federation, an annual decrease in SMR was noted, and in the rest — multidirectional changes. The increase in SMR from acute MI took place in 23 subjects of the Russian Federation with a minimum CV in 2020 and 2021 (44%). The maximum CV for SMD was recorded in 2021 (169%).

Conclusion. Mortality from acute types of CAD and SMD for 2019-2021 in the Russian Federation was characterized by a stable level and high regional variability. The problem of improving the criteria for establishing acute types of CAD and SMD as the initial cause of death remains relevant in the absence or insufficiency of data for universal criteria for MI.
Diagnostic challenges of heart failure with preserved ejection fraction: focus on echocardiography
Authors: Ivanova A. A. , Dzhioeva O. N. , Lavrenova E. A. , Rogozhkina E. A. , Drapkina O. M.
Published: June 2023

Aim. To assess the features of diagnosing heart failure with preserved ejection fraction (HFpEF) using echocardiographic markers and diastolic stress test (DST) according to a survey of ultrasound and functional diagnostics specialists in the Russian Federation.

Materials and methods. As part of the study, an anonymous survey of 155 ultrasound and functional diagnostic specialists from various Russian regions was conducted. We proposed to answer whether they routinely assess certain echocardiographic parameters necessary for diagnosing HFpEF. The specialists also indicated whether they conduct DST and whether they have the opportunity to refer the patient to this study.

Results. A frequency analysis of the responses received was carried out. In routine practice, 83,2% of specialists measure the left ventricular (LV) ejection fraction by Simpson method, 76,1% — by Teichholz method. In addition, 80% of responders analyses LV mass index, 76,1% — relative LV wall thickness, 60% — tricuspid annular plane systolic excursion, 56,8% — left atrial volume index, 51.6% — E/e´ ratio, 94,8% — pulmonary artery systolic pressure, left ventricular global longitudinal strain — 16,1%, 7,7% — left atrial longitudinal strain. Also, 9,7% of specialists conduct DST on their own, while 41,3% have the opportunity to refer patients.

Conclusion. The low assessment rate of some ultrasonic HFpEF mar­kers and DST among functional diagnostics specialists in the Russian Federation reduces the detection rate of HFpEF. It is necessary to develop diagnostic algorithms based mainly on clinical and anam­nestic data and available for use by doctors of any specialty.
A Kişilik Tipi ve İşyeri Mutluluğu İlişkisinde İçsel Motivasyonun Rolü
Authors: GÜNEY Salih , ERGİN Gülen
Published: June 2023

Çalışmada işyeri mutluluğu kavramının A kişilik tipine göre farklılık gösterip göstermediği ve bu bağlamda içsel motivasyonun rolü incelenmiştir. Bireyler dış görünüşleri ve zihinsel yapıları bir birinden farklıdır. Bu durumun doğal bir sonucu olarak da dış dünyayı algılayış biçimleri de bir birinden farklıdır. Kişilik kavramının esası bu unsurlardan meydana gelmektedir. Kişilik bireyin dış dünya ile olan münasebetini etkiler ve hislerini, düşünce ve eylemlerini yönlendirir. Bu çalışmada A kişilik tipi ele alınacaktır. İşyeri mutluluğu Türkiye’de akademik olarak az çalışılan ve işletmeler açısından düşündüğümüzde ise çoğunlukla üstünde fazla durulmayan bir kavramdır. Bununla beraber işyerinde mutlu olan çalışanın bulunduğu kuruma her anlamda katkı sağlaması beklenmektedir. Çalışanların mutluluğunu etki eden hisler ve bireyin kendisinde olduğunu düşündüğü potansiyeli gerçekleştirdiğine ilişkin fikri işyeri mutluluğu kavramının temelini meydana getirmektedir. Motivasyon kuramları uzun yıllardır araştırılan ve teorilerle açıklanmaya çalışılan kavramlardır. Çalışmamızda sadece içsel motivasyon ele alınmıştır. İçsel motivasyonu dışsal motivasyondan ayıran en önemli nokta motivasyon kaynağının bireyin kendisi ve iç dünyası olmasıdır. Bireylerde içsel motivasyonun dışsal motivasyondan daha etkili olması beklenmektedir. Bunun nedeni ödüllendirme gibi dışsal motivasyon faktörlerinin ortadan kalkması durumunda bireyin motivasyonunun kolayca düşmesidir. Araştırmada bireylerin işyerinde mutlu olma hallinin A kişilik tipi ve içsel motivasyon gibi işletme dışı faktörlerinden etkilenmesinin araştırılması bakımından literatüre katkısı bulunmaktadır. Araştırma öğretmenlere yönelik yapılmış olup 500 anketten 390 geçerli anket çalışmada kullanılmıştır. Araştırma sonuçlarına göre; A kişilik tipinin işyeri mutluluğuna doğrudan ve dolaylı etkisi bulunduğu saptanmıştır. Araştırma ile literatüre yapılan katkının yanı sıra insan kaynakları süreçlerinde kişilik yapısı motivasyon ve işyeri mutluluğu gibi kavramları dahil etmek isteyen kurumlara de katkı sağlanabilir. Araştırmanın örneklemini öğretmenler oluşturması nedeniyle elde edilmiş olan bulguların özellikle eğitim kurumlarına katkı sağlaması beklenmektedir.
Mechanical properties of aluminum through electronic- and atomic-scale simulations
Authors: Khani Somayeh , Palkowski Heinz
Published: June 2023

Implementing the integrated computational materials engineering methodology in modeling plastic deformation processes and associated phenomena can provide a unique opportunity for a deeper study of material behavior at smaller scales and result in more precise and accurate predictions. This study presents a multiscale modeling framework linking two different length scales – namely, the electronic and the atomic scale – to investigate the mechanical properties of pure aluminum (Al) and to achieve the required parameters and information for higher scales. At the electronic scale, the elastic properties and interfacial energies for aluminum were garnered from density functional theory simulations to calibrate the modified embedded atom method (MEAM) potentials required for atomic simulations. The calculation for the generalized stacking fault energy resulted in an intrinsic stacking fault energy of 185.5 mJ/m2. Using the parameter calculated at the electronic scale as well as the MEAM potential parameters, the edge dislocation mobility of aluminum from molecular dynamics simulations was calculated at the atomic scale (nano). A drag coefficient of 7.3 × 10−5 Pa s was computed at 300 K. The dependency of the drag coefficient on the temperature was also studied, and the results showed that the velocity linearly depended on τ/T up to 0.4 MPa/K.
Reflection of ethnopolitical concepts and scenarios of interethnic relations in the psychological meanings of abstract lexemes
Authors:   Institute of Philology of the Siberian Branch of the Russian Academy of Sciences, Shaposhnikova Irina V.
Published: June 2023

Ethnopolitical concepts and scenarios of interethnic relations among representatives of different interacting superethnic systems are reflected in the psychological meanings of words. The semantic structures of the associative fields (AF) of abstract nouns provide relevant, experimentally obtained data representing the psychological realia. The associative-verbal profiles can be regarded as statistical models of the psychological meanings behind the sound bodies of the analyzed lexemes. This study examines the associative fields of two abstract lexemes operating as explicators of dominating ethnopolitical attitudes in the contexts of interethnic relations to trace the semantic aspects of westernization of the young Russian students’ consciousness in the current diachrony (within the last 40–50 years). The analysis covers the latest databases obtained in the Asian regions (Siberia and the Far East) of the Russian Federation: SIBAS 1 (2008–2013) and SIBAS 2 (2014–2021), along with earlier materials: RAS (1988–1997). Psycholinguistic techniques allow us to trace the ways of embedding semantic components of ethnopolitical concepts coming from outside into the image of the world of the Russian language personality. The entries of newly coined or recently borrowed words in the explanatory dictionaries often fail to reflect the emerging lexicosemantic variants of their meanings. Hence, lexicographers can apply the knowledge obtained with the psycholinguistic toolkit as a complementary technique when describing the emerging meanings of recently borrowed words. The psychoglossic and vector analysis of the associative verbal fields allows the researcher to evaluate the dynamics and psychologically relevant trends in their meaning formation.
Resource conservation as the basis for the formation of Russia’s innovation infrastructure
Authors:   Russian State Academy of Intellectual Property of the Russian Federation, Smirnova Veronika R. , Kokurin Dmitry I. ,   Russian State Academy of Intellectual Property of the Russian Federation, Chernyavskiy Sergey V. ,   Central Economics and Mathematics Institute of the Russian Academy of Science, Vеtchinnikov Dmitry V. ,   Moscow Finance and Law Academy
Published: June 2023

Innovative infrastructure plays a supporting role, serving the country’s economy, facilitating the transfer of technology, the transformation of knowledge into new market-demanded goods and services. Creating an innovative infrastructure is a long process that requires large financial, human, and other resources. The article analyzes the problems associated with the formation and development of innovative infrastructure in the context of resource conservation. An approach is proposed that allows the use of resource-saving mechanisms in the formation of innovation infrastructure. An algorithm for creating a comprehensive resource-saving management mechanism at the macroeconomic level is described.
SUGARCANE MECHANIZED HARVEST QUALITY IN YIELD RANGES
Authors: Sousa Arthur Guilherme Garcia , Reis Maria Albertina Monteiro dos , Silva Edgard Henrique Costa
Published: June 2023

Based on information about the area in which sugarcane will be cultivated, yield ranges can be determined. The yield and cultivar adopted directly interfere in the harvest. Mechanized harvesting has advantages in the sugarcane production, but losses occur in this process. In this sense, the objective was to evaluate the mechanized harvesting quality of two sugarcane cultivars (CTC4 and SP86-0155) in three yield ranges (50 to 70, 71 to 90 and 91 to 120 t ha-1). The experiment was carried out in six areas in the region of the municipality of Jaboticabal-SP. In each area, yield and visible losses were determined at twelve points. The data were submitted to descriptive analysis and as a tool to analyze the harvest quality, the statistical process control (SPC) was used. The results indicate 54% higher losses in high yield ranges, and lower process quality. The cultivar SP86-0155 presented 11% less loss at harvest regarding to the cultivar CTC4, a fact that is related to the cultivars morphological characteristics.
História do Cinema, um Arquivo Aberto
Authors: Vieira da Silva Tiago
Published: June 2023

Recensão da segunda edição de História do Cinema: Dos Primórdios ao Cinema Contemporâneo (2023), obra coordenada por Nelson Araújo.
Novel 5,6,7,8-tetrahydrobenzo[b]pyran Derivatives: Synthesis and Anticancer Activity
Authors: Abdallah Amira E. M. , Mohareb Rafat M. , Helal Maher H. E. , Elkader Mariam M. Abd
Published: June 2023

Many new cyclized pyran systems with a potential anti-cancer activity were designed and prepared. Pyran systems showed high reactivity to various chemical reagents. 24 products of the prepared compounds were chosen and tested in (mM) as respectable anticancer factors. The findings revealed that compounds 3b, 6b, and 8 were the widely effective compounds against the three cancer cell lines including A-549 (lung carcinoma), HC-29 (colorectal adenocarcinoma), and MKN-45 (gastric cancer) compared to the standard reference control foretinib.
Phytochemical Profile, Antioxidant and Antimicrobial Potency of Aerial Parts of Salvia Tomentosa Miller
Authors: Balkır Şehnaz , Hazman Ömer , Aksoy Laçine , Yılmaz Mustafa Abdullah , Cakir Oguz , Kara Recep , Erol İbrahim
Published: June 2023

Antioxidant activity, antimicrobial potency and components of the aerial parts (leaf, stem, flower and mixture) of Salvia tomentosa Miller were determined qualitatively and quantitatively in this study. Aqueous extracts of Salvia tomentosa (ST) were prepared by using the flower, leaf and stem parts and all the above-ground parts of the plant (flower-leafstem mixture) for this purpose. The radical scavenging activity, total antioxidant/oxidant status, antimicrobial potential, phenolic substances and qualitative/quantitative analyzes of the components in the extracts were determined. ST-stem phenolic acid amount (599 ± 34 mg gallic acid equivalent (GAE)/g extract) was found to be close to the standard substance caffeic acid (651 ±3 1 mg GAE/g extract). Total antioxidant status of ST-mix (3.4 ± 0.1 mmol Trolox Equiv./L) and ST-stem (3.4 ± 0.1 mmol Trolox Equiv./L) and natural antioxidant Vitamin C (3.6 ± 0.1 mmol Trolox Equiv./L) were not statistically different. The extract produced by using S. tomentosa aerial parts (flower-stem-leaf) showed stronger antioxidant and antimicrobial activity than the aqueous extracts obtained separately from the flower, stem and leaf of the plant. However, it was determined that the components of the separately prepared flower, stem and leaf extracts and the extract components obtained from the aerial parts were largely similar. At the same time, it was observed that there were significant differences in the presence of these components.
Chemical and Antioxidant Profile of Hydroalcoholic Extracts of Stachys Officinalis L., Stachys Palustris L., Stachys Sylvatica L. from Romania
Authors: Apostolescu George Florian , Popescu Diana Ionela (Stegarus) , Botoran Oana , Sandru Daniela , Şuţan Nicoleta Anca , Neamtu Johny
Published: June 2023

Stachys officinalis L., Stachys palustris L., Stachys sylvatica L. (Lamiaceae) are widely used as herbal remedies. In this study, comparative assessment of the phenolic acids, flavonoids, anthocyanin, and tannins content, together with antioxidant activity of the extracts obtained from flowers, leaves and stems was performed. Phenolic acids determined by the HPLC method reached highest values in flower extract of S. palustris, stem extract of S. officinalis, and leaf extracts of S. sylvatica. Flavonoids were found at values exceeding 100 mg quercetin equivalents (QE)/g dry weights in all three species, based on the spectrophotometric method. Anthocyanins were detectable only in extracts from flowers. S. officinalis stood out for the highest content of anthocyanins and tannins. Antioxidant activity was present in all three species studied, with S. palustris standing out for the most intense ferric reducing antioxidant power. The results obtained lead to the validation of applicability of these plants for curative and food purposes, given their variety and richness in bioactive compounds and antioxidants.
Small-scale Expression and Solubility Testing of Proteins in BL21 E. coli v1
Authors: A Bielinski Vincent
Published: June 2023

Scaled-down solubility screen for bacterial overexpression constructs.
Active zone protein clusters and dopamine axons in striatal slices v1
Authors: Chen Chuyu
Published: June 2023

This protocol detail the procedures for measuring the size and density of active zone proteins and axon terminals with Imaris software.
Nuclei isolation from fresh frozen human colon tissue for 10X Genomics Multi-omics (ATAC + GEX) assay v1
Authors: not provided antonina.mikorska
Published: June 2023

Nuclei isolation protocol used for sn multi-omics assay on fresh frozen human colon tissue (full thickness and muscularis layer only).
Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP v1
Authors: Alvarez Jerez Pilar
Published: June 2023

Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP At the NIH's Center for Alzheimer's and Related Dementias (CARD)https://card.nih.gov/research-programs/long-read-sequencingwe will generate long-read sequencing data from roughly 4000 patients with Alzheimer's disease, frontotemporal dementia, Lewy body dementia, and healthy subjects. With this research, we will build a public resource consisting of long-read genome sequencing data from a large number of confirmed people with Alzheimer's disease and related dementias and healthy individuals. For this project the long-read sequencing data will be derived from brain tissue, but for benchmarking purposes we also developed a protocol for processing frozen cells. The frozen cell protocol was developed to generate long-read sequencing data for the Coriell human-genome cell-lines that is comparable to the data output of the brain based protocol described herehttps://www.protocols.io/view/processing-human-frontal-cortex-brain-tissue-for-p-kxygxzmmov8j/v2) i.e. targeting an N50 of ~30kb and ~30X coverage. †Correspondence to: Kimberley Billingsleybillingsleykj@nih.govand Cornelis Blauwendraatcornelis.blauwendraat@nih.gov Acknowledgements: We would like to thank the Nanopore team (Androo Markham &Hannah Lucio), Circulomics Inc team (Jeffrey Burke, Michelle Kim, Duncan Kilburn & Kelvin Liu) and the whole CARD long-read team listed below =>UCSC: Benedict Paten, Mikhail Kolmogorov, Miten Jain, Kishwar Shafin, Trevor Pesout;NHGRI: Adam Phillippy, Arang Rhie; Baylor: Fritz Sedlazeck;JHU: Winston Timp; NINDS: Sonja Scholz;NIA: Cornelis Blauwendraat, Kimberley Billingsley, Pilar Alvarez Jerez, Frank Grenn, Bryan Traynor, Shannon Ballard, Caroline Pantazis. Laksh Malik, Edmund Roberts;CZI: Paolo Carnevali.
Incell Scanning Protocol v1
Authors: not provided jwaligor
Published: June 2023

Incell Scanning Protocol
Purification and analysis ofSKP1-FBXO7 complexes v1
Authors: Adolf Frank
Published: June 2023

Protocol for the biochemical purification and analysis ofSKP1-FBXO7 complexes
Surgical procedures for simultaneous optogenetic manipulation in the SNc and acute fiber photometry in the dorsal striatum v1
Authors: Elena He Qianzi
Published: June 2023

This protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum: 1. Viral injection of a red-shifted channelrhodopsin, ChRmine, in the SNc, and a green dopamine sensor, GRAB-DA3m, in the dorsal striatum. 2. Fiber-optic cannula implantation into the SNc for optogenetic stimulation. 3. Craniotomy above the dorsal striatum for acute fiber photometry.
Human axillary lymph node fine-needle aspirate sample processing and cyropreservation v1
Authors: HY Siu Jacqueline
Published: June 2023

This protocol was used to generate a single-cell suspension from fine-needle aspirate samples of the adult human axillary lymph nodes and cyropreserved in CS10 for < 6 months. Cell yield was highly dependent on the participant; however, viability was >90% prior to freezing and between 80-90% after thawing (trypan blue dye exclusion).
Processing human frontal cortex brain tissue for population-scale Oxford Nanopore long-read DNA sequencing SOP v1
Authors: J Billingsley Kimberley
Published: June 2023

Processing human frontal cortex brain tissue for population-scale Oxford Nanopore long-read DNA sequencing SOP At the NIH's Center for Alzheimer's and Related Dementias (CARD) https://card.nih.gov/research-programs/long-read-sequencing we will generate long-read sequencing data from roughly 4000 patients with Alzheimer's disease, frontotemporal dementia, Lewy body dementia, and healthy subjects. With this research, we will build a public resource consisting of long-read genome sequencing data from a large number of confirmed people with Alzheimer's disease and related dementias and healthy individuals. To generate this large-scale nanopore sequencing data we have developed a protocol for processing and long-read sequencing human frontal cortex brain tissue, targeting an N50 of ~30kb and ~30X coverage. †Correspondence to: Kimberley Billingsley billingsleykj@nih.gov and Cornelis Blauwendraat cornelis.blauwendraat@nih.gov Acknowledgements: We would like to thank the Nanopore team (Androo Markham &Hannah Lucio), Circulomics Inc team (Jeffrey Burke, Michelle Kim, Duncan Kilburn & Kelvin Liu) and the whole CARD long-read team listed below => UCSC: Benedict Paten, Mikhail Kolmogorov, Miten Jain, Kishwar Shafin, Trevor Pesout; NHGRI: Adam Phillippy, Arang Rhie; Baylor: Fritz Sedlazeck; JHU: Winston Timp; NINDS: Sonja Scholz; NIA: Cornelis Blauwendraat, Kimberley Billingsley, Frank Grenn, Pilar Alvarez Jerez, Bryan Traynor, Shannon Ballard, Caroline Pantazis; CZI: Paolo Carnevali.
Working in AnVIL: A Clinical Sequencing Evidence-Generating Research (CSER) consortium perspective. v2
Authors: Green Richard
Published: June 2023

Analysis, Visualization, and Informatics Lab-space (AnVIL) is a powerful new Genomics Data science cloud-based platform. AnVIL features a variety of tools for data processing and analysis. This protocol will cover steps toward setting up an account, creating workspaces, customizing docker images, and accessing data and tools through R studio and the command line. We provide troubleshooting tips and best practices on resources, data storage, and monitoring costs. We also provide suggestions on requesting data access from dbGap through AnVIL.
The Limits of Social Media Mobilization: How Protest Movements Adapt to Social Media Logic
Authors: Schaaf Marlene , Quiring Oliver
Published: June 2023

The emergence of social networking sites offers protest movements new ways to mobilize for action and draw attention to their issues. However, relying on social media also creates challenges, as social media follow their own principles. If protest movements want to be visible in news feeds, they have to adapt to so-called social media logic, as originally postulated in mediatization research. The principles of social media have been conceptualized. However, there is a lack of empirical research on how political actors perceive and orient to this logic, how they learn about it, and the consequences for mobilization (i.e., communicating protest issues as well as taking protest action). As protest movements are an integral part of modern democracies, use social media somewhat intensively, and usually build on a fluid network structure that allows us to examine adaptation processes in greater detail, they are particularly suitable for addressing these questions. Semi-structured interviews with activists organizing protest actions or managing social media accounts from 29 movement organizations in Germany (N = 33) revealed that protest movements have internalized social media logic and paid attention to not only the design but also the timing of posts to suit algorithms. The protest organizations generally built on their experience with social media. The degree to which they followed these principles was based on available resources. Limits of this adaptation arose, for example, if sensitive or negative content rarely produced likes or, increasingly, personalization evoked a presumed hierarchy within the movements.
Digitalised Welfare: Access, Usage, and Outcomes Among Older Adults
Authors: Olsson Tobias , Viscovi Dino
Published: June 2023

The slogan “digital first” has become a buzzword for public organisational development at the local, regional, and national levels in Sweden. The slogan alludes to the idea that providing information to and communication with citizens should take place primarily through digital channels. This idea is also popular in other parts of the digitalised world. Obviously, digital solutions have the potential to become pedagogical, reliable, and effective interfaces for governmental interaction with citizens. However, the extent to which they are actually accessible, brought to use, and valued among older adult users (more than 65 years of age) has not attracted much research interest. Drawing on national survey data, collected in November–December 2020, on Swedish citizens (aged 65 to 90 years), this article will start to compensate for this deficit. First, it analyses the extent to which citizens have physical access to required devices and how access is related to material, discursive, and social resources. Second, it analyses resources and usage of important platforms for public services for older adults: the Pensions Agency, health care, e-pharmacy, the Social Insurance Agency, and the Tax Agency. Finally, the article examines outcomes: feelings and attitudes towards experiences of encountering a digitalising society. The article demonstrates how all three levels of divides—access, usage, and outcomes—are clearly related to older adults’ access to material, discursive, and social resources, as well as to age and pre-retirement experience with computers. It is comparatively younger individuals with longer formal education and at least average incomes and social networks who benefit the most from digitalised public services.
Clinical significance of varicocelectomy: literature review
Authors: Olefir Yu. V. , Efremov E. A. , Rodionov M. A. , Zhivulko A. R. , Popov D. M. , Monakov D. M. , Faniev M. V.
Published: June 2023

Surgical treatment of clinically significant varicocele in infertile men with pathospermia is a common practice. However, the question of the role of this disease in the pathogenesis of such conditions as hypogonadism and orchalgia, as well as the role of varicocelectomy in eliminating pain symptoms in the scrotum and increasing testosterone levels, has remained ambiguous for a long time. The expediency of surgical treatment of varicocele in patients with azoospermia also remains a subject of discussion.
Omalizumab in the treatment of various forms of chronic urticaria
Authors: Olisova Olga Yu. , Skander Dayana M.
Published: June 2023

BACKGROUND: Chronic urticaria is a skin disease characterized by the appearance of itchy weals and/or angioedema for 6 or more weeks. Chronic urticaria is subdivided into chronic spontaneous urticaria, which occurs due to an unknown cause, and chronic inducible urticaria, which occurs as a result of exposure to various physical factors (water, cold, heat, pressure, mechanical irritation), can occur simultaneously or independently of each other. Omalizumab, anti-IgE monoclonal antibody, is approved for the treatment of patients with chronic spontaneous urticaria and is the second choice in cases of resistance to antihistamine treatment. In patients with a combination of chronic spontaneous urticaria and chronic inducible urticaria, the effectiveness of treatment with omalizumab has been little studied.
AIM: compare the effectiveness of omalizumab treatment in patients with chronic spontaneous urticaria and patients with a combination of chronic spontaneous and chronic induced urticaria.
MATERIALS AND METHODS: Under supervision there were 30 patients with chronic spontaneous urticaria and combined chronic spontaneous and inducible urticaria (15 patients in each group). Evaluation of the effectiveness of treatment was carried out according to the results of the questionnaires DLQI (dermatological index of quality of life), CU-Q2oL (questionnaire for quality of life in chronic urticaria), UCT (urticaria control test), UAS (urticaria activity scale), HADS (Hospital Anxiety and Depression Scale) and provocation tests in dynamics before and during treatment.
RESULTS: All patients received omalizumab 300 mg subcutaneously once a month for 6 to 12 months. After the first injection of omalizumab, we noted a decrease in the severity of urticaria, an increase in the level of disease control and quality of life when comparing parameters before and during treatment in more than 90% of patients. Improved performance remained at this level throughout all subsequent months of treatment.
CONCLUSION: Omalizumab is equally effective in patients with an isolated form of chronic spontaneous urticaria and in patients with a combined form of chronic spontaneous and inducible urticaria. The use of omalizumab allows you to control the symptoms of chronic spontaneous and inducible urticaria, even with prolonged use.
Din ve Teknoloji Etkileşiminde Yapay Zeka ve Transhümanizm’e Yaklaşımlar
Authors: ACAR Ali Kemal
Published: June 2023

Yapay zekanın önemli bir değişimi başlatacağı kabul gören bir yaklaşımdır. Kültürleri, değerleri, talepleri, ihtiyaçları muhtemeldir ki hayatın tamamını derinden etkileyecek bir rol üstlenebilecektir. Transhümanizm ise teknolojiden aldığı güçle, evrimin devamını sağlayarak üst insanın inşa edilme iddiasıdır. Çalışmada teknoloji algıları yapay zeka özelinde belirlenmeye ve değerlendirmeye çalışılmıştır. Değişken yapısıyla teknolojinin, sabiteler barındıran din ile ilişkisi ve dine rağmen teknoloji olarak ifade edilebilecek Transhümanizm düşüncesi ele alınmıştır. Teknolojiye yaklaşımlar fazlaca olsa da karamsar ve itidalli yaklaşımların ağırlık kazandığı belirlenmiştir. Gelecek algısının distopyalar etrafında olumsuz şekillendiği belirtilebilir. Teknolojik gelişmeleri kısa vadede mübalağa etmeyen, uzun vadede ise küçümsemeyen bir mihengin önemli olduğu belirtilmelidir. Teknolojik gelişmeler dinin lehinde ya da aleyhinde kullanılagelmiştir. Bugün ve yarın için de bu kuralın işleyeceği kestirilebilir. Çalışmada nitel araştırma yöntemi kullanılmıştır. Doküman incelemesi ile veriler elde edilmiştir. Bu veriler içerik analizine tabi tutulmuştur. Karşılaştırma, değerlendirme, varsayım ve yorumlara gidilerek düşünce ve fikirler, eldeki veriler çerçevesinde araştırılmaya ve anlaşılmaya çalışılmıştır.
Impact of a multidisciplinary approach and patient routing on the outcomes of treatment of patients with hepatocellular cancer
Authors: Petkau V. V. , Bessonova E.  N. , Breder V. V. , Tarkhanov A.  A. , Kiseleva K. E.
Published: June 2023

Introduction: Hepatocellular cancer (HCC) is the sixth most common form of cancer worldwide and the third most common cause of cancer death. The long-term results of treatment are influenced by organizational decisions aimed at increasing the availability of specialized care. The purpose of our study is to evaluate the impact of rerouting and the introduction of a multidisciplinary approach on overall survival (OS) of patients with HCC. Materials and methods. A retrospective study was conducted to study the treatment tactics and results of treatment in all patients with HCC registered in the Sverdlovsk Regional Oncological Cancer Registry with a diagnosis of HCC from 2015 to 2021. A comparative analysis was carried out in 3 groups of patients: the control group, before changes, group of patients after changes in routing, and a group of patients with multidisciplinary approach involving a gastroenterologist/hepatologist and an interventional oncologist.

Results: There is an increase in the number of patients who received advice from an oncologist and referred for specialized treatment: 12.0 %, 19.5 % and 34.9 %, respectively (p < 0.001). Median OS increased significantly only in the multidisciplinary approach group of 3.1 versus 2.2 months (p = 0.002). Low overall survival rates are associated with a large proportion of late stages. A significant increase in OS was registered in the group of patients with BCLC C stage: from 3.4 months to 12.5 months (p = 0.046).

Conclusions: Simplifying the patient's route from a gastroenterologist to an oncologist shortens the time to treatment start, increases the number of patients who receive advisory assistance in an oncological dispensary, but does not affect OS. A multidisciplinary approach allows more patients to receive specialized care. At the same time, the maximum effect on OS is noted at the BCLC C stage.
Meta-analysis of studies on the effectiveness of adding anti-PD1 antibodies to the first-line chemotherapy in advanced esophageal cancer
Authors: Fedyanin M. Yu. , Tryakin A. A. , Rays A. B. , Kononets P. V. , Abou-Haidar O. B. , Gladilina I. A. , Malikhova O. A. , Tyulyandin S. A. , Stilidi I. S.
Published: June 2023

Introduction: The results of six randomized control trials have been published by 2022, which evaluated the efficacy of adding PD-1 inhibitors to the first-line chemotherapy in patients with metastatic esophageal cancer. However, it still remains unclear which patients derive the most clinical benefit from combined therapy. Therefore, it is necessary to evaluate the efficacy of the combination of anti-PD1 treatment with chemotherapy in different patient subgroups.

Materials and methods: We conducted a meta-analysis of randomized controlled trials in patients with stage IV esophageal cancer who received anti-PD1 drugs with different chemotherapeutic regimens in the first-line setting to select patients who benefit the most from the combined therapy.

Results: Six randomized trials were included in the analysis for a total of 3,813 patients. Patients who received combination therapy had significantly longer OS (HR = 0.69, 95% CI: 0.63-0.75; p < 0.001), PFS (HR = 0.62, 95% CI: 0.56-0.69; p < 0.001), and better ORR (OR = 2.12, 95% CI: 1.85-2.42; p < 0.001) than those who received chemotherapy alone. Subgroup analyses showed no benefit of adding PD1-inhibitors to chemotherapy in patients with PD-L1 CPS <1 in terms of OS (HR = 0.58, 95 % CI: 0.31-1.1; p = 0.1) as well as in never-smokers (HR = 0.9, 95% CI: 0.67-1.23; p = 0.52).

Conclusions: The addition of PD-1 inhibitors to the first-line chemotherapy in patients with metastatic esophageal cancer significantly improves treatment outcomes. Our results could not strongly suggest the selected patients' cohort which would benefit the most from the combination of PD-1 inhibitors and chemotherapy use.
Russian consensus on prevention, diagnosis and treatment of gastric cancer
Authors: Khatkov I. E. , Abdulkhakov S.  R. , Alekseenko S.  A. , Amelina I.  D. , Andreev D.  N. , Artamonova E.  V. , Bakulina N.  V. , Besova N.  S. , Bolotina L.  V. , Bordin D.  S. , Vasnev O.  S. , Vladimirova L.  Yu. , Galkova Z.  V. , Dolgushin B.  I. , Zhukova L.  G. , Ignatova E.  O. , Izrailov R.  E. , Isakov V.  A. , Kalinin A.  E. , Karachun A.  M. , Karmazanovskii G.  G. , Kashin S.  V. , Kachenko V.  A. , Kim V.  A. , Kononets P.  V. , Kostyuchenko L.  N. , Kuzmina T.  N. , Kulezneva Yu.  V. , Kurilovich S.  A. , Kucheryavii Yu.  A. , Livzan M.  A. , Lyadov V.  K. , Maev I.  V. , Malikhova O.  A. , Medvedeva B.  M. , Moiseenko V.  M. , Morozov S.  V. , Nedolugko I.  Yu. , Osipenko M.  F. , Parfenchikova E.  V. , Pasechnikov V.  D. , Pirogov S.  S. , Pokataev I.  A. , Protcenko S.  A. , Ryabov A.  B. , Semenov N.  E. , Solodinina E.  N. , Starkov Yu.  G. , Stilidi I.  S. , Stroyakovskii D.  L. , Tkachenko O.  B. , Tryakin A.  A. , Tjulandin S.  А. , Fedenko А.  А. , Fedyanin M.  Yu. , Homeriki S. G. , Homyakov V.  M. , Tsvirkun V.  V. , Tsukanov V.  V. , Shevkunov L.  N. , Shishin K.  V. , SHCHadrova V.  V. , Embutnieks Yu.  V.
Published: June 2023

The Russian consensus on prevention, diagnostic and treatment of gastric cancer was prepared on the initiative of the Moscow clinical scientific center named after A. S. Loginov according to the Delphi method. Its aim was to clarify and consolidate the opinions of specialists on the most relevant issues of prevention, diagnosis and treatment of gastric cancer. An interdisciplinary approach was provided by the participation of leading gastroenterologists, oncologists and surgeons.
Organ preservation treatment of patients with atypical hyperplasia and endometrial cancer: current trends
Authors: Petrozhitskaya A.  A. , Tandelov R.  K. , Zaitcev N.  A. , Morkhov K.  Yu.
Published: June 2023

The high prevalence and steady increase in the incidence of endometrial cancer dictate the need to prevent and improve the effectiveness of treatment of this disease. The solution of these issues is possible either by expanding the indications for surgical treatment in endometrial proliferative processes, or by improving conservative methods of therapy, which is of priority importance, since some patients often have the question of preserving reproductive function.

Leading oncogynecological communities have come to a consensus: conservative treatment with preservation of fertility is possible and safe for young patients with endometrioid endometrial cancer of a high degree of differentiation, limited to endometrial damage only. According to published studies, the use of progestins gives a high frequency response to treatment in patients with atypical hyperplasia and early endometrial cancer. Combined treatment methods, including the use of metformin or hysteroscopic resection after drug therapy, can reduce the frequency of relapses.

In the absence of larger prospective studies, it is very important to consider a woman's overall health and fertility potential before recommending conservative treatment for early endometrial cancer. It is necessary to conduct further randomized controlled trials in order to provide more convincing evidence of the benefits of a particular technique.
Development and Testing of a Mathematics Learning Application
Authors: Fadlil Arif Ahmad , Purbohadi Dwijoko , Riyadi Slamet
Published: June 2023

Distance learning, or online learning, is essential for both teachers and students as they work to create a system of education to function during a pandemic. Therefore, this research aims to develop a CAI media-based learning application for teaching fractions in mathematics. The survey results conducted at SDN 1 Sukerejo Boyolali revealed that students encountered the most trouble with fractions. Hence, their comprehension of the mathematics learning application was evaluated. The pre-test and post-test results of 30 students in both the control and experiment classes demonstrated that the application was easier to use than direct or traditional learning methods.
Aspek Keadilan Pengaturan Pemberian Ganti Rugi dalam Pengadaan Tanah untuk Pembangunan bagi Kepentingan Umum
Authors: Adhisukmawati I Gusti Ayu Kade Harry , Handayani I Gusti Ayu Ketut Rachmi , Karjoko Lego
Published: June 2023

The demand for land in the context of public-interest development is growing, but the supply of land is limited. Fairness and compensation mechanisms are usually challenging in the purchase of property for public use. The goal of this research is to look at the rule's fairness elements as well as the method for awarding compensation in the purchase of property for public development. This study is normative and prescriptive, with a regulatory and conceptual approach. The study's findings revealed that a) the fairness aspect in the regulation of land acquisition for development in the public interest must provide a guarantee of proper replacement, as stipulated in Law Number 2 of 2012 concerning Land Acquisition for Development in the Public Interest, Law Number 11 of 2020 concerning Job Creation, Indonesian Assessment Standard 204, and other land acquisition laws and regulations. And b) the compensation mechanism is implemented when the assessment is finished by the Indonesian Assessment Standard 204, Law Number 11 of 2020 about Job Creation, discussions, and validation activities. The payment can be made in Rupiah, as agreed upon by the Land Acquisition Committee and the community, and then transferred to a bank account designated by the government.
Aspek Hukum Perdata dalam Kontrak Bisnis Pariwisata dan Dampak Untuk Pembangunan Ekonomi di Indonesia
Authors: Perwira I Nyoman Satria , Handayani I Gusti Ayu Ketut Rachmi , Karjoko Lego
Published: June 2023

The tourism sector is experiencing a very rapid development by giving birth to various variations of tourism business activities. Where this tourism business activity sometimes experiences problems in carrying out tourism business contracts. This study aims to analyze the civil law aspects of tourism business contracts in Indonesia and the impact of tourism business on economic development in Indonesia.  This research is a normative research, prescriptive, with a regulatory and conceptual approach. The results of the study found that a) Aspects of civil law in the tourism business in Indonesia have an important role in it As we know tourism has tourist activities whose activities are a business. It is undeniable that a business relationship starts from a contract. In the absence of a contract, it is impossible for a business relationship to be carried out in which there is an agreement regulated in article 1313 of the Civil Code (Civil Code) known as an agreement.  And b)  The impact of the tourism business on economic development in Indonesia  in general, the greater the contribution of the tourism sector to the economy of a region, the greater the contribution of the tourism sector in improving the economic welfare of the people in the region.
Konstitusionalitas Rencana Perpanjangan Hak Guna Bangunan di Ibu Kota Nusantara
Authors: Maisari Rini , Ansharullah Ansharullah
Published: June 2023

The government plans to make a Government Regulation (PP) that would regulate the extension of Building Use Rights (HGB) in the Archipelago Capital City (IKN). This HGB later could be extended for 160 (one hundred and sixty) years. If passed, this PP is contrary to the Law Number 5 of 1960 concerning Basic Agrarian Regulations which regulates the extension of HGB only for 50 (fifty) years and contradicts to the Article 33 of the 1945 Constitution which prohibits monopolistic practices. This study aims to examine the constitutionality of the government's plan to extend the Right of Building in IKN. The results of the study showed that the plan for extending the Right of Building in IKN was constitutionally not in accordance with the principles of granting land rights as regulated in the Basic Agrarian Law or the principles of the agrarian constitution as regulated in Article 33 of the 1945 Constitution which requires the granting of land rights based on the principles of economic democracy and fair efficiency in which land rights are not only to fulfill individual preference but also social preference as manifested in norm regulation system that provides equal guarantees of rights to access land rights granted by the state and prevent the capitalism practice in land tenure.