bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychosocial and Biological Outcomes of Immersive, Mindfulness-Based Treks in Nature for Groups of Young Adults and Caregivers Affected by Cancer: Results from a Single Arm Program Evaluation from 2016–2021
Authors: Victorson David , Doninger Gretchen , Victorson Scott , Victorson Gwen , Hall Lars , Maletich Carly , Corr Bradley R. , Scortino Kathy , Burns Zachary , Allen Lori , Rosa Ian , Quirk Kelley , Adegbemi Adekunle , Strokoff Johanna , Zuidema Kile , Sajdak Kelle , Mckibben Todd , Roberts Angie , McDade Thomas W. , Boes Amanda , McAlinden Katie , Arredondo Karen , Sauer Christina , Smith Kristin , Salsman John M.
Published: December 2021

The COVID-19 pandemic has left many individuals suffering from “connection deficit disorder” given changes to the way we work, go to school, socialize, and engage in daily activities. Young adults affected by cancer between the ages of 18–39 have known this connection deficit long before the pandemic. Being diagnosed and treated for cancer during this time can significantly disrupt engagement in important educational, career, social, and reproductive pursuits, and contribute to increased stress, anxiety, depression, and other negative outcomes. Experiencing meaningful connection—with nature, with peers who understand, and with oneself, may help assuage this adverse effect of disconnect. A single arm within-subjects program evaluation was conducted to examine outcomes following participation in immersive, multi-night, mindfulness-based treks in nature in a sample of young adults (n = 157) and caregivers (n = 50) affected by cancer from 2016–2021. Pre to post-trek changes included significant (p < 0.001) self-reported improvements in feeling connected to nature (d = 0.93–0.95), peers (d = 1.1–1.3), and oneself (d = 0.57–1.5); significant (p < 0.001) improvements on PROMIS Anxiety (d = 0.62–0.78), Depression (d = 0.87–0.89), and Sleep Disturbance (d = 0.37–0.48) short forms; and significant (p < 0.05) changes in pro-inflammatory biomarkers (d = 0.55–0.82). Connection-promoting experiences like this have the potential to improve health and wellbeing in this population and serve as a model for others.
Tungsten Accumulation in Hot Spring Sediments Resulting from Preferred Sorption of Aqueous Polytungstates to Goethite
Authors: Zhao Qian , Guo Qinghai , Luo Li , Yan Ketao
Published: December 2021

Geothermal waters usually have elevated tungsten concentrations, making geothermal systems important sources of tungsten in the environment. To study the transport of tungsten in hot springs to hot spring sediment, which is one of the key processes for the release of geothermally derived tungsten to the surface environment, geochemical investigations of the hot springs and their corresponding sediments in Rehai (a representative hydrothermal area in southwestern China) and systematic laboratory experiments of tungstate and polytungstate adsorption onto typical iron-bearing minerals in hot spring sediments (i.e., pyrite and goethite) were conducted. The results demonstrate that considerable tungsten concentrations (i.e., not much less than 10 µg/L), formation of polytungstates under acidic conditions, and enrichment of iron oxide minerals represented by goethite are the prerequisites for extreme enrichment of tungsten in hot spring sediments (e.g., 991 µg/g in the ZZQ spring outflow channel). The absence of any of these conditions would weaken the immobilization of aqueous tungsten and result in higher mobility of tungsten in the hot springs and its further transport downstream, possibly polluting the other natural waters in and around Rehai that serve as local drinking water sources. This study provides an insight for identifying the key geochemical processes controlling the transport and fate of undesirable elements (in this case, tungsten) in geothermal systems.
Assessing Efficiency of Urban Land Utilisation under Environmental Constraints in Yangtze River Delta, China
Authors: Zhou Yue , Chen Yi , Hu Yi
Published: December 2021

Measuring the efficiency of construction land utilisation is important for optimising the allocation of regional resources and guiding the sustainable development of the regional society and economy. Based on municipal panel data on urban land use from 2009 to 2017 from a municipal perspective, this research built a slacks-based measure of a super-efficiency model (SE-SBM) to evaluate the temporal and spatial differentiation characteristics of the construction land-use efficiency of 41 cities in the Yangtze River Delta. Following this, the driving force of construction land efficiency was calculated using the Malmquist–Luenberger index. Finally, the entropy-weight TOPSIS (technique for order preference by similarity to ideal solution) model and the k-means clustering method were applied to evaluate an input–output model of the cities. The main conclusions are as follows: (1) The construction land efficiency of the Yangtze River Delta remains at a low level and presents a spatial differentiation pattern, with the efficiency being higher in the east and lower in the west. Due to undesired outputs, the mean value has dropped by 4.67%, and the regional imbalance has decreased. (2) The degree of efficiency loss is significantly positively correlated with the intensity of urban pollution emissions—the higher the pollution emissions, the greater the efficiency loss. (3) The total factor productivity of urban construction land is mainly driven by technological progress, while the promotion of technical efficiency is low and unstable. (4) The evaluation of construction land efficiency must include resource allocation or pollution emission factors to scientifically measure the input–output level. These research results will help to formulate reasonable land-use countermeasures.
The Influence of Episodic Future Thinking and Graphic Warning Labels on Delay Discounting and Cigarette Demand
Authors: Naudé Gideon P. , Dolan Sean B. , Strickland Justin C. , Berry Meredith S. , Cox David J. , Johnson Matthew W.
Published: December 2021

Delay discounting and operant demand are two behavioral economic constructs that tend to covary, by degree, with cigarette smoking status. Given historically robust associations between adverse health outcomes of smoking, a strong preference for immediate reinforcement (measured with delay discounting), and excessive motivation to smoke cigarettes (measured with operant demand), researchers have made numerous attempts to attenuate the extent to which behaviors corresponding to these constructs acutely appear in smokers. One approach is episodic future thinking, which can reportedly increase the impact of future events on present decision making as well as reduce the reinforcing value of cigarettes. Graphic cigarette pack warning labels may also reduce smoking by increased future orientation. Experiment 1 evaluated the combined effects of episodic future thinking and graphic warning labels on delay discounting; Experiment 2 evaluated solely the effects of episodic future thinking on delay discounting and operant demand. We observed no statistically significant effects of episodic future thinking when combined with graphic warning labels or when assessed on its own. These results serve as a call for further research on the boundary conditions of experimental techniques reported to alter behaviors associated with cigarette smoking.
COVID-19: Marking the Gaps in Migrant and Refugee Health in Some Massive Migration Areas
Authors: Matlin Stephen A. , Karadag Ozge , Brando Claudio R. , Góis Pedro , Karabey Selma , Khan Md. Mobarak Hossain , Saleh Shadi , Takian Amirhossein , Saso Luciano
Published: December 2021

The health of migrants and refugees, which has long been a cause for concern, has come under greatly increased pressure in the last decade. Against a background where the world has witnessed the largest numbers of migrants in history, the advent of the COVID-19 pandemic has stretched the capacities of countries and of aid, health and relief organizations, from global to local levels, to meet the human rights and pressing needs of migrants and refugees for access to health care and to public health measures needed to protect them from the pandemic. The overview in this article of the situation in examples of middle-income countries that have hosted mass migration in recent years has drawn on information from summaries presented in an M8 Alliance Expert Meeting, from peer-reviewed literature and from reports from international agencies concerned with the status and health of migrants and refugees. The multi-factor approach developed here draws on perspectives from structural factors (including rights, governance, policies and practices), health determinants (including economic, environmental, social and political, as well as migration itself as a determinant) and the human security framework (defined as “freedom from want and fear and freedom to live in dignity” and incorporating the interactive dimensions of health, food, environmental, economic, personal, community and political security). These integrate as a multi-component ‘ecological perspective’ to examine the legal status, health rights and access to health care and other services of migrants and refugees, to mark gap areas and to consider the implications for improving health security both for them and for the communities in countries in which they reside or through which they transit.
Rodent Model of Gender-Affirming Hormone Therapies as Specific Tool for Identifying Susceptibility and Vulnerability of Transgender People and Future Applications for Risk Assessment
Authors: Tassinari Roberta , Maranghi Francesca
Published: December 2021

Transgenders (TGs) are individuals with gender identity and behaviour different from the social norms; they often undergo gender-affirming hormone therapy (HT). HT for TG men involves testosterone treatment and, for TG women, oestrogen plus androgen-lowering agents. Due—but not limited—to the lifelong lasting HT, usually TG people experience several physical and behavioural conditions leading to different and specific susceptibility and vulnerability in comparison to general population, including the response to chemical contaminants present in daily life. In particular, the exposure to the widespread endocrine disrupters (EDs) may affect hormonal and metabolic processes, leading to tissue and organ damage. Since the endocrine system of TG people is overstimulated by HT and, often, the targets overlap with ED, it is reasonable to hypothesize that TG health deserves special attention. At present, no specific tools are available to study the toxicological effects of environmental contaminants, including EDs, and the potential long-term consequences of HT on TG people. In this context, the development of adequate and innovative animal models to mimic gender-affirming HT have a high priority, since they can provide robust data for hazard identification in TG women and men, leading to more reliable risk assessment.
A Novel Elderly Tracking System Using Machine Learning to Classify Signals from Mobile and Wearable Sensors
Authors: Muangprathub Jirapond , Sriwichian Anirut , Wanichsombat Apirat , Kajornkasirat Siriwan , Nillaor Pichetwut , Boonjing Veera
Published: December 2021

A health or activity monitoring system is the most promising approach to assisting the elderly in their daily lives. The increase in the elderly population has increased the demand for health services so that the existing monitoring system is no longer able to meet the needs of sufficient care for the elderly. This paper proposes the development of an elderly tracking system using the integration of multiple technologies combined with machine learning to obtain a new elderly tracking system that covers aspects of activity tracking, geolocation, and personal information in an indoor and an outdoor environment. It also includes information and results from the collaboration of local agencies during the planning and development of the system. The results from testing devices and systems in a case study show that the k-nearest neighbor (k-NN) model with k = 5 was the most effective in classifying the nine activities of the elderly, with 96.40% accuracy. The developed system can monitor the elderly in real-time and can provide alerts. Furthermore, the system can display information of the elderly in a spatial format, and the elderly can use a messaging device to request help in an emergency. Our system supports elderly care with data collection, tracking and monitoring, and notification, as well as by providing supporting information to agencies relevant in elderly care.
A Cross-Sectional Analysis of the Stigma Surrounding Type 2 Diabetes in Colombia
Authors: Pedrero Victor , Manzi Jorge , Alonso Luz Marina
Published: December 2021

Type 2 diabetes is a global epidemic, and many people feel stigmatized for having this disease. The stigma is a relevant barrier to diabetes management. However, evidence in this regard is scarce in Latin America. This study aimed to analyze the level of stigma surrounding type 2 diabetes in the Colombian population and its relationships with sociodemographic, clinical, psychosocial variables and behaviors related to management of the disease (self-management behaviors). This cross-sectional study included 501 Colombian adults with type 2 diabetes. We estimated the relation between stigma and selected variables through linear regression models. Additionally, we analyzed the mediator role of psychosocial variables in the relationship between stigma and self-management behaviors through structural equation models. A total of 16.4% of patients showed concerning levels of stigma. The time elapsed since diagnosis (β = −0.23) and socioeconomic status (β = −0.13) were significant predictors of the level of stigma. Stigma was negatively correlated with self-efficacy (β = −0.36), self-esteem (β = −0.37), and relationship with health care provider (β = −0.46), and positively correlated with stress (β = 0.23). Self-efficacy, self-esteem, and the relationships with health care providers had a mediation role in the relationship between stigma and self-management behaviors. These variables would be part of the mechanisms through which the perception of stigma harms self-management behaviors. The stigma of type 2 diabetes is frequent in the Colombian population and negatively associated with important aspects of disease management.
Associations between Socio-Economic Status and Unfavorable Social Indicators of Child Wellbeing; a Neighbourhood Level Data Design
Authors: van Minde Minke R. C. , de Kroon Marlou L. A. , Sijpkens Meertien K. , Raat Hein , Steegers Eric A. P. , Bertens Loes C. M.
Published: December 2021

Background: Living in deprivation is related to ill health. Differences in health outcomes between neighbourhoods may be attributed to neighbourhood socio-economic status (SES). Additional to differences in health, neighbourhood differences in child wellbeing could also be attributed to neighbourhood SES. Therefore, we aimed to investigate the association between neighbourhood deprivation, and social indicators of child wellbeing. Methods: Aggregated data from 3565 neighbourhoods in 390 municipalities in the Netherlands were eligible for analysis. Neighbourhood SES scores and neighbourhood data on social indicators of child wellbeing were used to perform repeated measurements, with one year measurement intervals, over a period of 11 years. Linear mixed models were used to estimate the associations between SES score and the proportion of unfavorable social indicators of child wellbeing. Results: After adjustment for year, population size, and clustering within neighbourhoods and within a municipality, neighbourhood SES was inversely associated with the proportion of ‘children living in families on welfare’ (estimates with two cubic splines: −3.59 [CI: −3.99; −3.19], and −3.00 [CI: −3.33; −2.67]), ‘delinquent youth’ (estimate −0.26 [CI: −0.30; −0.23]) and ‘unemployed youth’ (estimates with four cubic splines: −0.41 [CI: −0.57; −0.25], −0.58 [CI: −0.73; −0.43], −1.35 [−1.70; −1.01], and −0.96 [1.24; −0.70]). Conclusions: In this study using repeated measurements, a lower neighbourhood SES was significantly associated with a higher prevalence of unfavorable social indicators of child wellbeing. This contributes to the body of evidence that neighbourhood SES is strongly related to child health and a child’s ability to reach its full potential in later life. Future studies should consist of larger longitudinal datasets, potentially across countries, and should attempt to take the interpersonal variation into account with more individual-level data on SES and outcomes.
Effects of a Self-Regulated Training Program on the Repeated Power in Female College Handball Players: An Intervention Study
Authors: Espoz-Lazo Sebastián , Hinojosa-Torres Claudio , Farías-Valenzuela Claudio , Álvarez-Arangua Sebastián , Ferrero-Hernández Paloma , Valdivia-Moral Pedro
Published: December 2021

Several resistance training programs using conventional methodologies have been implemented with the purpose of improving the ability to perform power actions in handball, especially during the competitive season. In contrast, methodologies based on a contemporary perspective, which considers the human being as a self-regulating biological entity, and designed specifically for female college players, are scarce. The aim of this research was to investigate the effects of an eight-week resistance training program, in which the athletes were able to control the loads according to their self-perceived effort and rest on their repeated shuttle sprint and jump ability. The sample was composed of 16 female players of a handball team from the faculty of physics and mathematics sciences of a Chilean university. The RSSJA test was used to evaluate players’ conditions pre- and post-training program, and the self-perceived effort scale called OMNI-RES was used for the prediction and control of loads. Results indicated that, after the application of an eight-week resistance training program, significant improvements p ≤ 0.05 on the jump height (pre: 1836.4 W; average post: 2088.9 W) and running speed (average pre: 3.2 m/s; average post: 4.0 m/s) were obtained, as well as a significant reduction in the loss of power and speed between each set of the applied test.
Using an Integrated Social Cognition Model to Explain Green Purchasing Behavior among Adolescents
Authors: Pakpour Amir H. , Lin Cheng-Kuan , Safdari Mahdi , Lin Chung-Ying , Chen Shun-Hua , Hamilton Kyra
Published: December 2021

Strengthening pro-environmental behaviors such as green purchasing behavior is important for environmental sustainability. An integrated social cognition model which incorporates constructs from habit theory, health action process approach (HAPA), and theory of planned behavior (TPB) is adopted to understand Iranian adolescents’ green purchasing behavior. Using a correlational-prospective design, the study recruited Iranian adolescents aged between 14 and 19 years (N = 2374, n = 1362 (57.4%) females, n = 1012 (42.6%) males; Mean (SD) age = 15.56 (1.22)). At baseline (T1), participants self-reported on the following constructs: past behavior; habit strength (from habit theory); action planning and coping planning (from HAPA); and intention, perceived behavioral control, subjective norm, and attitude (from TPB) with respect to green purchasing behavior. Six months later (T2), participants self-reported on their actions in terms of purchasing green goods. Our findings reported direct effects of perceived behavioral control, subjective norms, attitude, and past behavior on intention; intention and perceived behavioral control on green purchase behavior; intention on two types of planning (i.e., action and coping planning); both types of planning on green purchase behavior; and past green purchase behavior and habits on prospectively measured green purchase behavior. These results indicate that adolescent green purchasing behavior is underpinned by constructs representing motivational, volitional, and automatic processes. This knowledge can help inform the development of theory-based behavior change interventions to improve green purchasing in adolescents, a key developmental period where climate change issues are salient and increased independence and demands in making self-guided decisions are needed.
Effect of Six-Minute Walk Test and Incremental Exercise on Inspiratory Capacity, Ventilatory Constraints, Breathlessness and Exercise Performance in Sedentary Male Smokers without Airway Obstruction
Authors: Melliti Wassim , Kammoun Rim , Masmoudi Donies , Ahmaidi Said , Masmoudi Kaouthar , Alassery Fawaz , Hamam Habib , Chlif Mehdi
Published: December 2021

We investigated physiological responses and exercise capacity in sedentary young smokers during sub-maximal and maximal test and its impact on dyspnea and exercise intolerance. Fifty sedentary male smokers and non-smokers (age: 24 ± 1 years., weight: 71 ± 9 kg, height: 177.3 ± 4.8 cm, body mass index: 22.6 ± 2.5 kg/m2) underwent two visits with pulmonary function tests, breathing pattern, and inspiratory capacity measurement at rest and during sub-maximal and maximal exercise. Smokers show reduced exercise capacity during six minutes walk test (6-MWT) with decreased walked distance (p < 0.001) and inspiratory capacity (p < 0.05). During cardiopulmonary exercise test (CPET), smokers had higher minute ventilation VE for a given submaximal intensity (p < 0.05) and lower minute ventilation at maximal exercise (p < 0.001). End expiratory lung volume was significantly lower in sedentary smokers at rest (p < 0.05), at ventilatory threshold during exercise (p < 0.05), but not during peak exercise. End inspiratory lung volume was significantly lower in smokers at rest (p < 0.05) and ventilatory threshold (p < 0.05). Cigarette smoking alters lung function during submaximal and maximal exercise. This alteration is manifested by the development of dynamic hyperinflation contributing to exercise capacity limitation.
The Acute Relationship between Affective States and Stress Biomarkers in Ethnic Minority Youths
Authors: Wen Cheng K. Fred , Chou Chih-Ping , Belcher Britni R. , Weigensberg Marc J. , Black David S. , Spruijt-Metz Donna
Published: December 2021

Background: Whether affective states acutely predict the hypothalamic–pituitary–adrenal (HPA) axis activities and whether energy balance-related behaviors moderate the affect–HPA axis relationship in obese youths are not well-understood. Methods: 87 mostly obese (94.3% obese) minority adolescents (mean: 16.3 ± 1.2 years old; 56.8% Latino and 43.2% African American) participated in a randomized crossover trial in an observation laboratory, where they received either high-sugar/low-fiber (HSLF) or low-sugar/high-fiber (LSHF) meals first and then crossed over in the next visit 2–4 weeks later. During each visit, they rated five affective states and provided a saliva sample every 30 min for the first 5 h and wore a waist-worn accelerometer. The association between the affect ratings and cortisol levels in the subsequent 30 min and the moderation effect of energy balance-related behavior were examined using multilevel models. Results: Within-person negative affect (β = 0.02, p = 0.0343) and feeling of panic (β = 0.007, p = 0.004) were acutely related to the subsequent cortisol level only during the HSLF condition. The time spent in moderate-to-vigorous physical activity did not moderate the acute relationship between affect and the subsequent cortisol level. Conclusions: Negative affect could be acutely related to heightened HPA axis activities in youths, but only when they were exposed to meals with high sugar and low fiber content. These results suggest that the meals’ sugar and fiber content may modulate HPA axis reactivity to negative affect in youths.
Dynamic Grid-Based Spatial Density Visualization and Rail Transit Station Prediction
Authors: Cai Zhi , Ji Meilin , Mi Qing , Yang Bowen , Su Xing , Guo Limin , Ding Zhiming
Published: December 2021

The urban rail transit stations are an important part of an urban transit system. Scientific and reasonable location of rail transit station can greatly alleviate traffic pressure. The number of people in the surrounding area of a rail transit station is an important factor for site selection. However, it is difficult to obtain the spatial distribution of population, which brings great difficulties in terms of site selection. Due to the large-scale popularization of AP (Access Point) in China, the spatial distribution of AP is used instead of population distribution to assist site selection. Therefore, a density visualization method based on a dynamic grid is proposed, which can help decision-makers intuitively see the AP density of the uncovered grid of rail transit stations, and then cluster the AP density of the uncovered area to predict the location of new rail transit stations. The validity of the proposed method is demonstrated by using the AP dataset and rail transit data of Beijing in 2013. The results show that our method has high accuracy in predicting the location of rail transit stations. It can provide data support for urban traffic development and management.
OBIA-Based Extraction of Artificial Terrace Damages in the Loess Plateau of China from UAV Photogrammetry
Authors: Fang Xuan , Li Jincheng , Zhu Ying , Cao Jianjun , Na Jiaming , Jiang Sheng , Ding Hu
Published: December 2021

Terraces, which are typical artificial landforms found around world, are of great importance for agricultural production and soil and water conservation. However, due to the lack of maintenance, terrace damages often occur and affect the local flow process, which will influence soil erosion. Automatic high-accuracy mapping of terrace damages is the basis of monitoring and related studies. Researchers have achieved artificial terrace damage mapping mainly via manual field investigation, but an automatic method is still lacking. In this study, given the success of high-resolution unmanned aerial vehicle (UAV) photogrammetry and object-based image analysis (OBIA) for image processing tasks, an integrated framework based on OBIA and UAV photogrammetry is proposed for terrace damage mapping. The Pujiawa terrace in the Loess Plateau of China was selected as the study area. Firstly, the segmentation process was optimised by considering the spectral features and the terrains and corresponding textures obtained from high-resolution images and digital surface models. The feature selection was implemented via correlation analysis, and the optimised segmentation parameter was achieved using the estimation of scale parameter algorithm. Then, a supervised k-nearest neighbourhood classifier was used to identify the terrace damages in the segmented objects, and additional geometric features at the object level were considered for classification. The comparison with the ground truth, as delineated by the image and field survey, showed that proposed classification can be adequately performed. The F-measures of extraction on three terrace damages were 92.07% (terrace sinkhole), 81.95% (ridge sinkhole), and 85.17% (collapse), and the Kappa coefficient was 85.34%. Finally, the potential application and spatial distribution of the terrace damages in this study were determined. We believe that this work can provide a credible framework for mapping terrace damages in the Loess Plateau of China.
Analyzing Contextual Linking of Heterogeneous Information Models from the Domains BIM and UIM
Authors: Beck Stefan F. , Abualdenien Jimmy , Hijazi Ihab H. , Borrmann André , Kolbe Thomas H.
Published: December 2021

Information models from the domains Building Information Modeling (BIM) and Urban Information Modeling (UIM) are generally considered as information silos due to their heterogeneous character. These information silos can be bridged through linking where corresponding objects are identified and linked subsequently. However, whether two objects are considered as corresponding might depend on the scenario for which the links are created. The dependency of the link creation and the scenario refers to the term contextual linking and is analyzed in this paper with respect to building and city models. Therefore, different situational aspects influencing the link creation are discussed. Afterwards, the issue of contextual linking is demonstrated based on three different integration scenarios. In summary, this paper has three major outcomes: First, this paper introduces an application-oriented perspective on information integration and emphasize the role of the application when linking heterogeneous information models. Second, this paper shows that linking heterogeneous information models from the domains BIM and UIM at instance level depends on the scenario. Third, the results of the discourse about contextual linking serve as a framework supporting the design and development of artifacts for linking heterogeneous information models from the domains BIM and UIM.
Instance Segmentation for Governmental Inspection of Small Touristic Infrastructure in Beach Zones Using Multispectral High-Resolution WorldView-3 Imagery
Authors: de Carvalho Osmar Luiz Ferreira , de Moura Rebeca dos Santos , de Albuquerque Anesmar Olino , de Bem Pablo Pozzobon , de Castro Pereira Rubens , Weigang Li , Borges Dibio Leandro , Guimarães Renato Fontes , Gomes Roberto Arnaldo Trancoso , de Carvalho Júnior Osmar Abílio
Published: December 2021

Misappropriation of public lands is an ongoing government concern. In Brazil, the beach zone is public property, but many private establishments use it for economic purposes, requiring constant inspection. Among the undue targets, the individual mapping of straw beach umbrellas (SBUs) attached to the sand is a great challenge due to their small size, high presence, and agglutinated appearance. This study aims to automatically detect and count SBUs on public beaches using high-resolution images and instance segmentation, obtaining pixel-wise semantic information and individual object detection. This study is the first instance segmentation application on coastal areas and the first using WorldView-3 (WV-3) images. We used the Mask-RCNN with some modifications: (a) multispectral input for the WorldView3 imagery (eight channels), (b) improved the sliding window algorithm for large image classification, and (c) comparison of different image resizing ratios to improve small object detection since the SBUs are small objects (<322 pixels) even using high-resolution images (31 cm). The accuracy analysis used standard COCO metrics considering the original image and three scale ratios (2×, 4×, and 8× resolution increase). The average precision (AP) results increased proportionally to the image resolution: 30.49% (original image), 48.24% (2×), 53.45% (4×), and 58.11% (8×). The 8× model presented 94% AP50, classifying nearly all SBUs correctly. Moreover, the improved sliding window approach enables the classification of large areas providing automatic counting and estimating the size of the objects, proving to be effective for inspecting large coastal areas and providing insightful information for public managers. This remote sensing application impacts the inspection cost, tribute, and environmental conditions.
Myelinosome Organelles in the Retina of R6/1 Huntington Disease (HD) Mice: Ubiquitous Distribution and Possible Role in Disease Spreading
Authors: Yefimova Marina G. , Béré Emile , Cantereau-Becq Anne , Meunier-Balandre Annie-Claire , Merceron Bruno , Burel Agnès , Merienne Karine , Ravel Célia , Becq Frédéric , Bourmeyster Nicolas
Published: December 2021

Visual deficit is one of the complications of Huntington disease (HD), a fatal neurological disorder caused by CAG trinucleotide expansions in the Huntingtin gene, leading to the production of mutant Huntingtin (mHTT) protein. Transgenic HD R6/1 mice expressing human HTT exon1 with 115 CAG repeats recapitulate major features of the human pathology and exhibit a degeneration of the retina. Our aim was to gain insight into the ultrastructure of the pathological HD R6/1 retina by electron microscopy (EM). We show that the HD R6/1 retina is enriched with unusual organelles myelinosomes, produced by retinal neurons and glia. Myelinosomes are present in all nuclear and plexiform layers, in the synaptic terminals of photoreceptors, in the processes of retinal neurons and glial cells, and in the subretinal space. In vitro study shows that myelinosomes secreted by human retinal glial Müller MIO-M1 cells transfected with EGFP-mHTT-exon1 carry EGFP-mHTT-exon1 protein, as revealed by immuno-EM and Western-blotting. Myelinosomes loaded with mHTT-exon1 are incorporated by naive neuronal/neuroblastoma SH-SY5Y cells. This results in the emergence of mHTT-exon1 in recipient cells. This process is blocked by membrane fusion inhibitor MDL 28170. Conclusion: Incorporation of myelinosomes carrying mHTT-exon1 in recipient cells may contribute to HD spreading in the retina. Exploring ocular fluids for myelinosome presence could bring an additional biomarker for HD diagnostics.
Deficiency of T-Cell Intracellular Antigen 1 in Murine Embryonic Fibroblasts Is Associated with Changes in Mitochondrial Morphology and Respiration
Authors: Carrascoso Isabel , Velasco Beatriz Ramos , Izquierdo José M.
Published: December 2021

T-cell intracellular antigen 1 (TIA1) is a multifunctional RNA-binding protein involved in regulating gene expression and splicing during development and in response to environmental stress, to maintain cell homeostasis and promote survival. Herein, we used TIA1-deficient murine embryonic fibroblasts (MEFs) to study their role in mitochondria homeostasis. We found that the loss of TIA1 was associated with changes in mitochondrial morphology, promoting the appearance of elongated mitochondria with heterogeneous cristae density and size. The proteomic patterns of TIA1-deficient MEFs were consistent with expression changes in molecular components related to mitochondrial dynamics/organization and respiration. Bioenergetics analysis illustrated that TIA1 deficiency enhances mitochondrial respiration. Overall, our findings shed light on the role of TIA1 in mitochondrial dynamics and highlight a point of crosstalk between potential pro-survival and pro-senescence pathways.
Alternative RAS in Various Hypoxic Conditions: From Myocardial Infarction to COVID-19
Authors: Rajtik Tomas , Galis Peter , Bartosova Linda , Paulis Ludovit , Goncalvesova Eva , Klimas Jan
Published: December 2021

Alternative branches of the classical renin–angiotensin–aldosterone system (RAS) represent an important cascade in which angiotensin 2 (AngII) undergoes cleavage via the action of the angiotensin-converting enzyme 2 (ACE2) with subsequent production of Ang(1-7) and other related metabolites eliciting its effects via Mas receptor activation. Generally, this branch of the RAS system is described as its non-canonical alternative arm with counterbalancing actions to the classical RAS, conveying vasodilation, anti-inflammatory, anti-remodeling and anti-proliferative effects. The implication of this branch was proposed for many different diseases, ranging from acute cardiovascular conditions, through chronic respiratory diseases to cancer, nonetheless, hypoxia is one of the most prominent common factors discussed in conjugation with the changes in the activity of alternative RAS branches. The aim of this review is to bring complex insights into the mechanisms behind the various forms of hypoxic insults on the activity of alternative RAS branches based on the different duration of stimuli and causes (acute vs. intermittent vs. chronic), localization and tissue (heart vs. vessels vs. lungs) and clinical relevance of studied phenomenon (experimental vs. clinical condition). Moreover, we provide novel insights into the future strategies utilizing the alternative RAS as a diagnostic tool as well as a promising pharmacological target in serious hypoxia-associated cardiovascular and cardiopulmonary diseases.
Macrophage Activation in the Dorsal Root Ganglion in Rats Developing Autotomy after Peripheral Nerve Injury
Authors: Xu Xiang , Zhou Xijie , Du Jian , Liu Xiao , Qing Liming , Johnson Blake N. , Jia Xiaofeng
Published: December 2021

Autotomy, self-mutilation of a denervated limb, is common in animals after peripheral nerve injury (PNI) and is a reliable proxy for neuropathic pain in humans. Understanding the occurrence and treatment of autotomy remains challenging. The objective of this study was to investigate the occurrence of autotomy in nude and Wistar rats and evaluate the differences in macrophage activation and fiber sensitization contributing to the understanding of autotomy behavior. Autotomy in nude and Wistar rats was observed and evaluated 6 and 12 weeks after sciatic nerve repair surgery. The numbers of macrophages and the types of neurons in the dorsal root ganglion (DRG) between the two groups were compared by immunofluorescence studies. Immunostaining of T cells in the DRG was also assessed. Nude rats engaged in autotomy with less frequency than Wistar rats. Autotomy symptoms were also relatively less severe in nude rats. Immunofluorescence studies revealed increased macrophage accumulation and activation in the DRG of Wistar rats. The percentage of NF200+ neurons was higher at 6 and 12 weeks in Wistar rats compared to nude rats, but the percentage of CGRP+ neurons did not differ between two groups. Additionally, macrophages were concentrated around NF200-labeled A fibers. At 6 and 12 weeks following PNI, CD4+ T cells were not found in the DRG of the two groups. The accumulation and activation of macrophages in the DRG may account for the increased frequency and severity of autotomy in Wistar rats. Our results also suggest that A fiber neurons in the DRG play an important role in autotomy.
Topoisomerase IIIβ Deficiency Induces Neuro-Behavioral Changes and Brain Connectivity Alterations in Mice
Authors: Rahman Faiz Ur , Kim You-Rim , Kim Eun-Kyeung , Kim Hae-rim , Cho Sang-Mi , Lee Chin-Soo , Kim Su Jin , Araki Kimi , Yamamura Ken-ichi , Lee Mi-Ni , Park Seul Gi , Yoon Won-Kee , Lee Kihoon , Won Young-Suk , Kim Hyoung-Chin , Lee Younghee , Lee Ho-Young , Nam Ki-Hoan
Published: December 2021

Topoisomerase IIIβ (Top3β), the only dual-activity topoisomerase in mammals that can change topology of both DNA and RNA, is known to be associated with neurodevelopment and mental dysfunction in humans. However, there is no report showing clear associations of Top3β with neuropsychiatric phenotypes in mice. Here, we investigated the effect of Top3β on neuro-behavior using newly generated Top3β deficient (Top3β−/−) mice. We found that Top3β−/− mice showed decreased anxiety and depression-like behaviors. The lack of Top3β was also associated with changes in circadian rhythm. In addition, a clear expression of Top3β was demonstrated in the central nervous system of mice. Positron emission tomography/computed tomography (PET/CT) analysis revealed significantly altered connectivity between many brain regions in Top3β−/− mice, including the connectivity between the olfactory bulb and the cerebellum, the connectivity between the amygdala and the olfactory bulb, and the connectivity between the globus pallidus and the optic nerve. These connectivity alterations in brain regions are known to be linked to neurodevelopmental as well as psychiatric and behavioral disorders in humans. Therefore, we conclude that Top3β is essential for normal brain function and behavior in mice and that Top3β could be an interesting target to study neuropsychiatric disorders in humans.
Cytochrome P450 Enzymes and Drug Metabolism in Humans
Authors: Zhao Mingzhe , Ma Jingsong , Li Mo , Zhang Yingtian , Jiang Bixuan , Zhao Xianglong , Huai Cong , Shen Lu , Zhang Na , He Lin , Qin Shengying
Published: December 2021

Human cytochrome P450 (CYP) enzymes, as membrane-bound hemoproteins, play important roles in the detoxification of drugs, cellular metabolism, and homeostasis. In humans, almost 80% of oxidative metabolism and approximately 50% of the overall elimination of common clinical drugs can be attributed to one or more of the various CYPs, from the CYP families 1–3. In addition to the basic metabolic effects for elimination, CYPs are also capable of affecting drug responses by influencing drug action, safety, bioavailability, and drug resistance through metabolism, in both metabolic organs and local sites of action. Structures of CYPs have recently provided new insights into both understanding the mechanisms of drug metabolism and exploiting CYPs as drug targets. Genetic polymorphisms and epigenetic changes in CYP genes and environmental factors may be responsible for interethnic and interindividual variations in the therapeutic efficacy of drugs. In this review, we summarize and highlight the structural knowledge about CYPs and the major CYPs in drug metabolism. Additionally, genetic and epigenetic factors, as well as several intrinsic and extrinsic factors that contribute to interindividual variation in drug response are also reviewed, to reveal the multifarious and important roles of CYP-mediated metabolism and elimination in drug therapy.
Dominant Role of PI3K p110α over p110β in Insulin and β-Adrenergic Receptor Signalling
Authors: Zhang Biqin , Luk Cheukyau , Valadares Joyce , Aronis Christos , Foukas Lazaros C.
Published: December 2021

Attribution of specific roles to the two ubiquitously expressed PI 3-kinase (PI3K) isoforms p110α and p110β in biological functions they have been implicated, such as in insulin signalling, has been challenging. While p110α has been demonstrated to be the principal isoform activated downstream of the insulin receptor, several studies have provided evidence for a role of p110β. Here we have used isoform-selective inhibitors to estimate the relative contribution of each of these isoforms in insulin signalling in adipocytes, which are a cell type with essential roles in regulation of metabolism at the systemic level. Consistent with previous genetic and pharmacological studies, we found that p110α is the principal isoform activated downstream of the insulin receptor under physiological conditions. p110α interaction with Ras enhanced the strength of p110α activation by insulin. However, this interaction did not account for the selectivity for p110α over p110β in insulin signalling. We also demonstrate that p110α is the principal isoform activated downstream of the β-adrenergic receptor (β-AR), another important signalling pathway in metabolic regulation, through a mechanism involving activation of the cAMP effector molecule EPAC1. This study offers further insights in the role of PI3K isoforms in the regulation of energy metabolism with implications for the therapeutic application of selective inhibitors of these isoforms.
Pan-Genome Reverse Vaccinology Approach for the Design of Multi-Epitope Vaccine Construct against Escherichia albertii
Authors: Jalal Khurshid , Khan Kanwal , Ahmad Diyar , Hayat Ajmal , Basharat Zarrin , Abbas Muhammad Naseer , Alghamdi Saad , Almehmadi Mazen , Sahibzada Muhammad Umar Khayam
Published: December 2021

Escherichia albertii is characterized as an emerging pathogen, causing enteric infections. It is responsible for high mortality rate, especially in children, elderly, and immunocompromised people. To the best of our knowledge, no vaccine exists to curb this pathogen. Therefore, in current study, we aimed to identify potential vaccine candidates and design chimeric vaccine models against Escherichia albertii from the analysis of publicly available data of 95 strains, using a reverse vaccinology approach. Outer-membrane proteins (n = 4) were identified from core genome as vaccine candidates. Eventually, outer membrane Fimbrial usher (FimD) protein was selected as a promiscuous vaccine candidate and utilized to construct a potential vaccine model. It resulted in three epitopes, leading to the design of twelve vaccine constructs. Amongst these, V6 construct was found to be highly immunogenic, non-toxic, non-allergenic, antigenic, and most stable. This was utilized for molecular docking and simulation studies against six HLA and two TLR complexes. This construct can therefore be used for pan-therapy against different strains of E. albertii and needs to be tested in vitro and in vivo.
PIAS Factors from Rainbow Trout Control NF-κB- and STAT-Dependent Gene Expression
Authors: Sarais Fabio , Kummerow Sophia , Montero Ruth , Rebl Henrike , Köllner Bernd , Goldammer Tom , Collet Bertrand , Rebl Alexander
Published: December 2021

Four ‘protein inhibitors of activated STAT’ (PIAS) control STAT-dependent and NF-κB-dependent immune signalling in humans. The genome of rainbow trout (Oncorhynchus mykiss) contains eight pias genes, which encode at least 14 different pias transcripts that are differentially expressed in a tissue- and cell-specific manner. Pias1a2 was the most strongly expressed variant among the analysed pias genes in most tissues, while pias4a2 was commonly low or absent. Since the knock-out of Pias factors in salmonid CHSE cells using CRISPR/Cas9 technology failed, three structurally different Pias protein variants were selected for overexpression studies in CHSE-214 cells. All three factors quenched the basal activity of an NF-κB promoter in a dose-dependent fashion, while the activity of an Mx promoter remained unaffected. Nevertheless, all three overexpressed Pias variants from trout strongly reduced the transcript level of the antiviral Stat-dependent mx gene in ifnγ-expressing CHSE-214 cells. Unlike mx, the overexpressed Pias factors modulated the transcript levels of NF-κB-dependent immune genes (mainly il6, il10, ifna3, and stat4) in ifnγ-expressing CHSE-214 cells in different ways. This dissimilar modulation of expression may result from the physical cooperation of the Pias proteins from trout with differential sets of interacting factors bound to distinct nuclear structures, as reflected by the differential nuclear localisation of trout Pias factors. In conclusion, this study provides evidence for the multiplication of pias genes and their sub-functionalisation during salmonid evolution.
Root-to-Shoot Long-Distance Mobile miRNAs Identified from Nicotiana Rootstocks
Authors: Deng Zhuying , Wu Huiyan , Li Dongyi , Li Luping , Wang Zhipeng , Yuan Wenya , Xing Yongzhong , Li Chengdao , Liang Dacheng
Published: December 2021

Root-derived mobile signals play critical roles in coordinating a shoot’s response to underground conditions. However, the identification of root-to-shoot long-distance mobile signals has been scant. In this study, we aimed to characterize root-to-shoot endogenous mobile miRNAs by using an Arabidopsis/Nicotiana interfamilial heterograft in which these two taxonomically distant species with clear genetic backgrounds had sufficient diversity in differentiating miRNA sources. Small RNA deep sequencing analysis revealed that 82 miRNAs from the Arabidopsis scion could travel through the graft union to reach the rootstock, whereas only a very small subset of miRNA (6 miRNAs) preferred the root-to-shoot movement. We demonstrated in an ex vivo RNA imaging experiment that the root-to-shoot mobile Nb-miR164, Nb-miR395 and Nb-miR397 were targeted to plasmodesmata using the bacteriophage coat protein MS2 system. Furthermore, the Nb-miR164 was shown to move from the roots to the shoots to induce phenotypic changes when its overexpressing line was used as rootstock, strongly supporting that root-derived Nb-miR164 was able to modify the scion trait via its long-distance movement.