bluuebunny/crossref_metadata_2025 huggingface dataset:
From existing conventional building towards LEED certified green building: case study in Bangladesh
Authors: Iqbal Ashik , Jahan Ismat , Wasiew Qudrati Al , Emu Imtiaz Ahmed , Chowdhury Dipta
Published: September 2023

Green Building refers to the planning, design, construction, and operation of buildings considering indoor environment quality, occupant health, using energy, water, and other resources more efficiently; and reducing waste, pollution, and the overall environmental impact. Among the Green Building guidelines, Leadership in Energy and Environmental Design (LEED) offers a certification and rating system by which buildings are certified in different categories. The LEED rating system is used to rate an existing building how much it is fulfilling the criteria of Green Building (GB) or not. This research explored how an existing conventional building can be retrofitted to satisfy green building standards. An academic building of KUET has been selected for the study. A field survey and Participatory Rural Appraisal (PRA) tools (i.e., Focus Group Discussion (FGD), Key Informant Interview (KII)) were used for the data collection and then the data was analyzed by comparative analysis concerning the LEED 2009 rating system for assessing the building. The New Academic building receives 31 points out of 110 and so cannot earn any certification level. The lacking for which the points were not gained is identified and the installation of water and energy efficiency features, rooftop gardening is suggested as retrofitting options to earn the green building certification level.
WeChat online visual language among Chinese Gen Z: virtual gift, aesthetic identity, and affection language
Authors: Liu Ruoxi
Published: September 2023

Introduction

As a relatively new and innovative form of digital communication and visual image, emojis, emoticons, and stickers are appreciated, yet criticised. Young people on social media have lost the traditional social etiquette and interpersonal networking skills, which is a challenge in itself. This study provides a better understanding of how young people engage and behave in diverse interpersonal contexts while utilising digital visual language.

Methods

This study contributes to digital anthography and social media studies, by conducting this study through interviews with 10 Generation Z young people from urban China and a 2-month-long participatory observation of three WeChat group chats.

Results

By examining how young people use WeChat visual language in relationships with their elders or superiors, equivalent peers and intimate lovers or friends, this study found that emoticons and stickers become virtual gifts, aesthetic identities and the affection language of Generation Z.

Discussion

This study contends that digital visual languages and new media do not alter human nature or ethical standards but rather provide new avenues for expression and empower human affective communication. Although the core of traditional etiquette is still being passed on and absorbed, its form has changed.
Peripheral and central macrophages in obesity
Authors: Mukherjee Sayani , Skrede Silje , Haugstøyl Martha , López Miguel , Fernø Johan
Published: September 2023

Obesity is associated with chronic, low-grade inflammation. Excessive nutrient intake causes adipose tissue expansion, which may in turn cause cellular stress that triggers infiltration of pro-inflammatory immune cells from the circulation as well as activation of cells that are residing in the adipose tissue. In particular, the adipose tissue macrophages (ATMs) are important in the pathogenesis of obesity. A pro-inflammatory activation is also found in other organs which are important for energy metabolism, such as the liver, muscle and the pancreas, which may stimulate the development of obesity-related co-morbidities, including insulin resistance, type 2 diabetes (T2D), cardiovascular disease (CVD) and non-alcoholic fatty liver disease (NAFLD). Interestingly, it is now clear that obesity-induced pro-inflammatory signaling also occurs in the central nervous system (CNS), and that pro-inflammatory activation of immune cells in the brain may be involved in appetite dysregulation and metabolic disturbances in obesity. More recently, it has become evident that microglia, the resident macrophages of the CNS that drive neuroinflammation, may also be activated in obesity and can be relevant for regulation of hypothalamic feeding circuits. In this review, we focus on the action of peripheral and central macrophages and their potential roles in metabolic disease, and how macrophages interact with other immune cells to promote inflammation during obesity.
The newly proposed alanine aminotransferase to high-density lipoprotein cholesterol ratio has shown effectiveness in identifying non-alcoholic fatty liver disease
Authors: Qiu Jiajun , Kuang Maobin , Yang Ruijuan , Yu Changhui , He Shiming , Sheng Guotai , Zou Yang
Published: September 2023

Objective

Alanine aminotransferase (ALT) and high-density lipoprotein cholesterol (HDL-C) are important predictive factors for non-alcoholic fatty liver disease (NAFLD). The aim of this study was to analyze the association between the ALT/HDL-C ratio and NAFLD.

Methods

We conducted a retrospective analysis of data from 14,251 individuals participating in the NAGALA project’s health screening program. The presence of NAFLD was diagnosed based on the participants’ alcohol consumption status and liver ultrasonography images. Multivariable logistic regression models were used to assess the association between the ALT/HDL-C ratio and NAFLD. Receiver operating characteristic (ROC) analysis was performed to determine and compare the effectiveness of ALT, HDL-C, the aspartate aminotransferase to HDL-C (AST/HDL-C) ratio, the gamma-glutamyl transferase to HDL-C (GGT/HDL-C) ratio and the ALT/HDL-C ratio in identifying NAFLD.

Results

We observed a significant positive association between the ALT/HDL-C ratio and the prevalence of NAFLD. For each standard deviation (SD) increase in the ALT/HDL-C ratio, the adjusted odds ratio (OR) for NAFLD among the participants was 3.05 [95% confidence interval (CI): 2.63, 3.53], with the highest quartile of ALT/HDL-C ratio having a 9.96-fold increased risk compared to the lowest quartile. In further subgroup analyses stratified by gender, age, and waist circumference (WC), we observed a significantly higher risk of NAFLD associated with the ALT/HDL-C ratio among individuals aged ≥45 years, males, and those who were abdominal obesity. Furthermore, based on the results of ROC analysis, we found that the ALT/HDL-C ratio [area under the curves (AUC): 0.8553] was significantly superior to ALT, HDL-C, AST/HDL-C ratio and GGT/HDL-C ratio in identifying NAFLD (All Delong P<0.05); the threshold of suggested ALT/HDL-C ratio for identifying NAFLD was 15.97.

Conclusion

This population-based study demonstrates a positive association between the ALT/HDL-C ratio and NAFLD. The ALT/HDL-C ratio can effectively identify individuals with NAFLD.
Landslide susceptibility mapping and management in Western Serbia: an analysis of ANFIS- and SVM-based hybrid models
Authors: Elkhrachy Ismail , Ranjan Yadav Rajeev , Nouh Mabdeh Ali , Nguyen Thanh Phong , Spalevic Velibor , Dudic Branislav
Published: September 2023

Landslide susceptibility mapping (LSM) is essential for land-use planning, as it helps to identify areas at risk of landslides and enables effective prevention measures to be taken. Various statistical and machine learning (ML) models are used in LSM, including SVM and ANFIS, which have shown promising results. However, determining which model performs better remains a key challenge. To address this issue, this paper aims to compare six hybrid models constructed with two well-known and powerful ML models, namely SVM and ANFIS, and three meta-heuristic algorithms, namely Genetic Algorithm (GA), Differential Evolution (DE), and Cultural Algorithm (CA), for LSM in a case study in western Serbia. In the process of building the models, 359 landslide sites and 14 determinants were used. The accuracy of the models was evaluated using several indexes, including Root Mean-Squared Error (RMSE), coefficient of determination (R2), and Area under the Receiver Operating Characteristic Curve (AUROC). The modeling results showed that the SVM-GA model has the highest accuracy (AUROC = 0.78) in predicting landslide incidence, followed by the ANFIS-GA (AUROC = 0.775), SVM-CA (AUROC = 0.773), ANFIS-DE (AUROC = 0.771), SVM-DE (AUROC = 0.76), and ANFIS-CA (AUROC = 0.65) models in validation phase. Therefore, the study suggested that SVM-based hybrid models are more accurate than ANFIS-based models for LSM, and thus, modelers may use SVM-based hybrid models for such applications. This study provides valuable insights into identifying the most appropriate and effective models for LSM, which can help to mitigate the risks associated with landslides and ensure sustainable land-use practices.
Adaptive shift of active electroreception in weakly electric fish for troglobitic life
Authors: Soares Daphne , Gallman Kathryn , Bichuette Maria Elina , Fortune Eric S.
Published: September 2023

The adaptive-shift hypothesis for the evolution of cave-dwelling species posits that ancestor species in surface habitats had exaptations for subterranean life that were exploited when individuals invaded caves. Weakly electric Gymnotiform fishes, nocturnal South American teleost fishes, have features that appear to be likely exaptations for troglobitic life. These fishes have active electrosensory systems in which fish generate weak electric fields that are detected by specialized electroreceptors. Gymnotiform fishes use their electric fields for navigation, prey capture (scene analysis), and social communication. Although active electrosensory systems appear to be exaptations for troglobitic life, as fish use these systems to “see in the dark”, producing electric fields is energetically costly. Cave habitats, which often are low in resources, may not be able to support such high energetic demands. Eigenmannia vicentespelaea, a species of weakly electric fish that is endemic to the São Vicente II cave in central Brazil, surprisingly generates stronger electric fields than their surface relatives. The increase in strength of electric fields may result simply from differences in size between cave and surface populations, but may also be due to lack of predation pressure in the cave or increases in “sensory volumes” and acuity that improve prey localization and capture. Eigenmannia vicentespelaea exhibits the classical phenotypes of any troglobitic fish: these fish have small to nonexistent eyes and loss of pigmentation. The closest living surface relative, Eigenmannia trilineata, inhabits streams nearby and has eyes and pigmentation. The electrosensory and locomotor behavior of both species of fish were measured in their natural habitats using a grid recording system. Surface Eigenmannia exhibited dramatic circadian changes in social behavior, such as hiding under rocks during the day and foraging in groups at night, while cave Eigenmannia displayed territorial behavior with no apparent circadian modulations. The territorial behavior involved electrical and movement-based interactions that may be a form of boundary patrolling. Electrosocial behavior and scene analysis are mechanistically interlinked because both stem from active sensing tactics.
Prognostic utility of TME-associated genes in pancreatic cancer
Authors: Nie Yuanhua , Xu Longwen , Bai Zilong , Liu Yaoyao , Wang Shilong , Zeng Qingnuo , Gao Xuan , Xia Xuefeng , Chang Dongmin
Published: September 2023

Background: Pancreatic cancer (PC) is a deadly disease. The tumor microenvironment (TME) participates in PC oncogenesis. This study focuses on the assessment of the prognostic and treatment utility of TME-associated genes in PC.

Methods: After obtaining the differentially expressed TME-related genes, univariate and multivariate Cox analyses and least absolute shrinkage and selection operator (LASSO) were performed to identify genes related to prognosis, and a risk model was established to evaluate risk scores, based on The Cancer Genome Atlas (TCGA) data set, and it was validated by external data sets from the Gene Expression Omnibus (GEO) and Clinical Proteomic Tumor Analysis Consortium (CPTAC). Multiomics analyses were adopted to explore the potential mechanisms, discover novel treatment targets, and assess the sensitivities of immunotherapy and chemotherapy.

Results: Five TME-associated genes, namely, FERMT1, CARD9, IL20RB, MET, and MMP3, were identified and a risk score formula constructed. Next, their mRNA expressions were verified in cancer and normal pancreatic cells. Multiple algorithms confirmed that the risk model displayed a reliable ability of prognosis prediction and was an independent prognostic factor, indicating that high-risk patients had poor outcomes. Immunocyte infiltration, gene set enrichment analysis (GSEA), and single-cell analysis all showed a strong relationship between immune mechanism and low-risk samples. The risk score could predict the sensitivity of immunotherapy and some chemotherapy regimens, which included oxaliplatin and irinotecan. Various latent treatment targets (LAG3, TIGIT, and ARID1A) were addressed by mutation landscape based on the risk model.

Conclusion: The risk model based on TME-related genes can reflect the prognosis of PC patients and functions as a novel set of biomarkers for PC therapy.
A serum metabolic biomarker panel for early rheumatoid arthritis
Authors: Rodríguez-Muguruza Samantha , Altuna-Coy Antonio , Arreaza-Gil Verónica , Mendieta-Homs Marina , Castro-Oreiro Sonia , Poveda-Elices Maria José , del Castillo-Piñol Nuria , Fontova-Garrofé Ramon , Chacón Matilde R.
Published: September 2023

Objective

There is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.

Methods

We applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.

Results

Significant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.

Conclusion

A metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
Pentameric C-reactive protein is a better prognostic biomarker and remains elevated for longer than monomeric CRP in hospitalized patients with COVID-19
Authors: Hopkins Francis R. , Nordgren Johan , Fernandez-Botran Rafael , Enocsson Helena , Govender Melissa , Svanberg Cecilia , Svensson Lennart , Hagbom Marie , Nilsdotter-Augustinsson Åsa , Nyström Sofia , Sjöwall Christopher , Sjöwall Johanna , Larsson Marie
Published: September 2023

The differing roles of the pentameric (p) and monomeric (m) C-reactive protein (CRP) isoforms in viral diseases are not fully understood, which was apparent during the COVID-19 pandemic regarding the clinical course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Herein, we investigated the predictive value of the pCRP and mCRP isoforms for COVID-19 severity in hospitalized patients and evaluated how the levels of the protein isoforms changed over time during and after acute illness. This study utilized samples from a well-characterized cohort of Swedish patients with SARS-CoV-2 infection, the majority of whom had known risk factors for severe COVID-19 and required hospitalization. The levels of pCRP were significantly raised in patients with severe COVID-19 and in contrast to mCRP the levels were significantly associated with disease severity. Additionally, the pCRP levels remained elevated for at least six weeks post inclusion, which was longer compared to the two weeks for mCRP. Our data indicates a low level of inflammation lasting for at least six weeks following COVID-19, which might indicate that the disease has an adverse effect on the immune system even after the viral infection is resolved. It is also clear that the current standard method of testing pCRP levels upon hospitalization is a useful marker for predicting disease severity and mCRP testing would not add any clinical relevance for patients with COVID-19.
Dendrochronology reveals different effects among host tree species from feeding by Lycorma delicatula (White)
Authors: Dechaine Andrew C. , Pfeiffer Douglas G. , Kuhar Thomas P. , Salom Scott M. , Leskey Tracy C. , McIntyre Kelly C. , Walsh Brian , Speer James H.
Published: September 2023

The spotted lanternfly, Lycorma delicatula (White) (Hemiptera: Fulgoridae), was first detected in the United States in Berks County, Pennsylvania, in 2014. Native to China, this phloem-feeding planthopper threatens agricultural, ornamental, nursery, and timber industries in its invaded range through quarantine restrictions on shipments, as well as impacts on plants themselves. The long-term impacts of L. delicatula feeding on tree species have not been well studied in North America. Using standard dendrochronological methods on cores taken from trees with differing levels of L. delicatula infestation and systemic insecticidal control, we quantified the impact of L. delicatula feeding on the annual growth of four tree species in Pennsylvania: Ailanthus altissima, Juglans nigra, Liriodendron tulipifera, and Acer rubrum. The results suggest that L. delicatula feeding is associated with the diminished growth of A. altissima, but no change was observed in any other tree species tested. The results also suggest that systemic insecticides mitigate the impact of L. delicatula feeding on A. altissima growth.
New ecophysiological perspectives on the kelp Macrocystis pyrifera: generating a basis for sustainability in the sub-Antarctic region
Authors: Marambio Johanna , Rodríguez Juan Pablo , Rosenfeld Sebastián , Méndez Fabio , Ojeda Jaime , Ocaranza Paula , Bischof Kai , Mansilla Andrés
Published: September 2023

These extensive kelps forest are among the most productive and diverse habitats on the planet, playing an important ecological role in marine ecosystems. These habitats have been affected by anthropogenic factors worldwide and directly by environmental variations resulting from climate change. The Magellan ecoregion has the southernmost kelp forests in the world, dominated by the species Macrocystis pyrifera. This species presents high ecophysiological plasticity being able to inhabit heterogeneous environments, characteristic of the fjord and channel systems of the region, and has high ecological, sociocultural, and economic importance for local coastal communities. To understand the ecophysiological acclimation strategies of M. pyrifera, samples from different blades were collected at different depths at four locations in the Magellan Ecoregion: Possession Bay, Skyring Sound, Otway Sound, and Puerto del Hambre seasonally. Abiotic measurements (salinity, temperature, and PAR light) were carried out for each location sampled. Measurements of photosynthetic parameters, Fv/Fm, rETRmax, Ek and α; pigment analysis of Chl a, Chl c, and fucoxanthin; and fecundity analysis of the sporophylls of each population studied were carried out on the M. pyrifera sporophytes. Significant differences were observed between seasons, locality, and depth of blades. Each population generally showed different photoacclimation processes, depending on the local conditions such as salinity values and probably tidal cycles. This is reflected in the photosynthetic, pigment, and fecundity values obtained during this study. The higher Fv/Fm values in all populations during the winter and autumn seasons and the differences in Chl c and fucoxanthin concentration during the winter period in Otway Sound and Puerto del Hambre population suggest the marked seasonal acclimation of M. pyrifera. In addition, the coastal environmental heterogeneity observed in the Magellan ecoregion related to salinity gradients (Skyring Sound) or wide tidal amplitudes (Possession Bay) influences the acclimation strategy of each population of M. pyrifera. Therefore, the characteristics of each population should be considered in order to promote its sustainability in times of social and climate change.
Optimizing rheological properties for printability: low-temperature extrusion 3D printing of hydroxyapatite-polycaprolactone mixture inks for bone tissue engineering
Authors: Yan Ming , Awad Hani A.
Published: September 2023

Introduction: Low-temperature extrusion three-dimensional printing (LTE-3DP) using viscous ceramic-polymer inks has shown promise for bone tissue engineering. This process involves formulating a flowable ink by combining ceramic powders and other components with organic or inorganic polymer solutions, which can then be extruded through a 3D printer nozzle. LTE-3DP allows the incorporation of high fractions of bioactive ceramics and thermally labile additives such as drugs, proteins, and biomolecules into the inks to promote osteogenesis and bone regeneration. The rheology of the ink, influenced by various variables, significantly impacts the printability and form fidelity of the resulting scaffolds. These variables include the composition of the polymer solution and the size and weight ratio of ceramic microparticles. In this study, we posited that the printability of hydroxyapatite (HA) and polycaprolactone (PCL) mixture inks could be optimized by tailoring their rheological properties.

Methods: We conducted a systematic investigation, varying the PCL weight percentage and HA:PCL weight ratio, to examine the effects of the ink’s composition on its viscosity and storage modulus, as well as its printability and the mechanical properties of 3D printed HA:PCL scaffolds.

Results: We demonstrated that HA:PCL inks exhibit predictable non-Newtonian fluid behavior at higher fractions of HA, displaying significant shear thinning at elevated shear rates, which can facilitate extrusion through a 3D printing nozzle. We identified printable ink compositions based on filament continuity and scaffold form fidelity criteria. Moreover, we performed computational simulations to analyze the ink flow through an extrusion nozzle. These simulations utilized the Herschel-Bulkley-Papanastasiou constitutive model, considering the rheological properties obtained from experimental measurements. By combining experimental measurements and computational simulations, we formulated a non-dimensional Printability number that predicts whether an ink is printable based on the ink’s rheological parameters and printer-specific factors. Furthermore, we evaluated the compressive properties of printed HA:PCL scaffolds and characterized the effects of PCL% and HA:PCL ratio on the hyperelasticity observed in response to compressive deformations.

Discussion: This hybrid approach using experimental rheology and FE simulations provides a framework to define the printability of ceramic-polymer ink formulations, which could help streamline the 3D printing of novel inks for bone tissue engineering.
The impact of COVID-19 pandemic on orthodontic services and trainees’ mental health in India
Authors: Thiruvenkatachari Badri , Sivakumar Prema , Ananth Sanjana , Sabbagh Yana , Lewis Benjamin R. K. , Chadwick Stephen M. , Kaliyaperumal Gnana Shanmugam , Batra Panchali
Published: September 2023

Aim

To evaluate the impact of COVID-19 on clinical orthodontic services, orthodontic education, and the emotional well-being of orthodontists and orthodontic trainees in India.

Materials and methods

The survey was designed using Survey Monkey™ and distributed to members of the Indian Orthodontic Society via their registered email address and also via social media platforms (WhatsApp and LinkedIn).

Results

A total of 610 responses to the survey were received. The majority of respondents agreed on the negative impact of COVID-19 on clinical activity and the associated income of orthodontists. Respondents reported that this was mainly due to national restrictions (70.1%), increased cross infection measures (59.6%), state restrictions (55.9%), and social distancing (39.4%). Ninety one percent of respondents agreed that orthodontic staff should have evidence of vaccination before providing care.

COVID-19 was found to have a negative impact on the trainees’ perceptions of their clinical dexterity (72.4%), their confidence with respect to academic knowledge (66.5%), their mental health (80.7%), and their stress levels during the pandemic (93.2%).

Conclusion

The COVID-19 pandemic has had a negative impact on orthodontic specialists and post-graduate trainees in India. The impact on trainees’ mental health was significantly higher compared to trainees from other countries. Decreased clinical activity has reduced the opportunities for learning, and trainers must rise to the challenge of providing additional support to this cohort of trainees who will progress to become the future orthodontic workforce.
JAK1 inhibitor abrocitinib for the treatment of steroid-induced rosacea: case series
Authors: Xu Bingyang , Xu Zining , Ye Shuhong , Sun Hong , Zhao Bin , Wu Na , Wu Jiawen
Published: September 2023

Background

Steroid-induced rosacea is a severe withdrawal reaction which can occur after the frequent and excessive topical use of steroids on the face. The Janus kinase (JAK)-signal transducers and activators of transcription signaling pathway is involved in many biological processes and may play a role in the development of steroid-induced rosacea.

Objective

To observe the efficacy and safety of the JAK1 inhibitor abrocitinib in the treatment of steroid-induced rosacea.

Methods

Four Chinese female patients were treated with orally-administered abrocitinib, a selective JAK1 inhibitor with a good safety profile, for steroid-induced rosacea.

Results

Abrocitinib treatment resulted in improved skin condition and lowered Dermatology Life Quality Index scores in each of the four patients. No discomfort was reported and no adverse effects were observed.

Conclusion

The JAK1 inhibitor abrocitinib is a promising potential treatment for steroid-induced rosacea.
Case report: A pediatric case of repeated false-positive urea breath test for Helicobacter pylori without decreased gastric acid secretion
Authors: Nishino Masafumi , Kakiuchi Toshihiko , Fukuda Kayoko , Yoshiura Masato
Published: September 2023

The urea breath test (UBT) is often used to diagnose Helicobacter pylori infection and for its eradication. However, this text can give positive results even for other urease-active bacteria other than H. pylori. Even after the successful eradication of H. pylori, the presence of other urease-active bacteria in the gut and oral cavity can lead to positive UBT results in patients with decreased gastric acid secretion. Herein, a 15-year-old boy was diagnosed with H. pylori infection through the testing and treatment program for H. pylori for third-year junior high-school students in Saga Prefecture initiated in 2016. He underwent triple therapy comprising vonoprazan; however, UBT was found to be positive even after therapy. The results remained positive even after fourth-line eradication therapy. Stool antigen, PCR using gastric fluid, microscopy, culture, and rapid urease tests were all negative. Pepsinogen levels were normal, and none of the findings suggested autoimmune gastritis. Gastric microflora analysis revealed oral flora showing urease activity. UBT is considered useful for determining the successful eradication of H. pylori; however, it may give false-positive results for both H. pylori infection and eradication judgment. Although the patient did not have autoimmune gastritis or decreased gastric acid secretion, it is presumed that oral commensal bacteria showing urease activity inhabited the stomach, resulting in the persistently positive UBT results. In conclusion, repeated false-positive UBT results for H. pylori may occur even without gastric acid hyposecretion. If H. pylori eradication is unsuccessful based on UBT, additional test by stool H. pylori antigen tests should be considered.
Application of targeted diagnosis of PSMA in the modality shift of prostate cancer diagnosis: a review
Authors: Yan Li , Zhang Zhengchao , Wang Ting , Yuan Leihong , Sun Xiaoke , Su Pengxiao
Published: September 2023

Prostate cancer (PCa) is a serious threat to the health of men all over the world. The progression of PCa varies greatly among different individuals. In clinical practice, some patients often progress to advanced PCa. Therefore, accurate imaging for diagnosis and staging of PCa is particularly important for clinical management of patients. Conventional imaging examinations such as MRI and CT cannot accurately diagnose the pathological stages of advanced PCa, especially metastatic lymph node (LN) stages. As a result, developing an accurate molecular targeted diagnosis is crucial for advanced PCa. Prostate specific membrane antigen (PSMA) is of great value in the diagnosis of PCa because of its specific expression in PCa. At present, researchers have developed positron emission tomography (PET) targeting PSMA. A large number of studies have confirmed that it not only has a higher tumor detection rate, but also has a higher diagnostic efficacy in the pathological stage of advanced PCa compared with traditional imaging methods. This review summarizes recent studies on PSMA targeted PET in PCa diagnosis, analyzes its value in PCa diagnosis in detail, and provides new ideas for urological clinicians in PCa diagnosis and clinical management.
Prognostic significance of lactate dehydrogenase and its impact on the outcomes of gastric cancer: a systematic review and meta-analysis
Authors: Chen Jinjin , Zou Xiaoyue
Published: September 2023

Background

The prognostic significance of lactate dehydrogenase (LDH) and its impact on the outcomes of gastric cancer (GC) is still unclear. We assessed the link between the levels of LDH and the overall survival (OS) and disease-free survival (DFS) in GC patients.

Methods

A comprehensive search (both electronic and manual) was carried out in PubMed via MEDLINE, Web of Science (WoS), Experta Medical Database (Embase), and CENTRAL (Cochrane Library) databases for citations that evaluated the strength of association between LDH cut-off levels and OS and/or DFS in GC. Pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated using a random-effects model, and heterogeneity was assessed.

Results

Eighteen studies with 5328 patients were included in our review. The overall pooled HR for OS was 1.48 (95% CI: 1.22-1.80) with high heterogeneity (I2 = 86%). Subgroup analyses showed that the link between LDH and OS was more prominent in Caucasian (HR 1.50 95% CI [0.80, 2.81], p=0.21) than in Asian cohorts (HR, 1.51 95% CI [1.21, 1.87], p=0.002). No significant overall association between LDH and OS (HR = 1.12, 95% CI: 0.76-1.65, p = 0.58) was found. Similar subgroup analyses results were reported for the association between LDH and DFS.

Conclusion

In patients with GC, elevated LDH levels may correlate with worse OS and DFS, but the association is not significant. LDH is a significant predictor of OS but not of DFS. Further studies with larger sample sizes and more standardized criteria for defining elevated LDH levels are needed to confirm our findings.

Systematic review registration

https://www.crd.york.ac.uk/prospero, identifier CRD42023412449.
Regulation of iron metabolism and ferroptosis in cancer stem cells
Authors: Wang Hailiang , Zhang Zhongyan , Ruan Shiye , Yan Qian , Chen Yubin , Cui Jinwei , Wang Xinjian , Huang Shanzhou , Hou Baohua
Published: September 2023

The ability of cancer stem cells (CSCs) to self-renew, differentiate, and generate new tumors is a significant contributor to drug resistance, relapse, and metastasis. Therefore, the targeting of CSCs for treatment is particularly important. Recent studies have demonstrated that CSCs are more susceptible to ferroptosis than non-CSCs, indicating that this could be an effective strategy for treating tumors. Ferroptosis is a type of programmed cell death that results from the accumulation of lipid peroxides caused by intracellular iron-mediated processes. CSCs exhibit different molecular characteristics related to iron and lipid metabolism. This study reviews the alterations in iron metabolism, lipid peroxidation, and lipid peroxide scavenging in CSCs, their impact on ferroptosis, and the regulatory mechanisms underlying iron metabolism and ferroptosis. Potential treatment strategies and novel compounds targeting CSC by inducing ferroptosis are also discussed.
Effects of acupuncture on gut microbiota and short-chain fatty acids in patients with functional constipation: a randomized placebo-controlled trial
Authors: Yan Xiang-Yun , Yao Jun-Peng , Li Yan-Qiu , Xiao Xian-Jun , Yang Wan-Qing , Chen Si-Jue , Tang Tai-Chun , Yang Yu-Qing , Qu Liu , Hou Yu-Jun , Chen Min , Li Ying
Published: September 2023

Objective: To comprehensively evaluate the effect of acupuncture on gut microbiota, identify specific microbes closely related to the clinical efficacy of acupuncture, and explored the role of short-chain fatty acids (SCFAs).

Methods: A randomized placebo-controlled trial was conducted with 80 FC patients and 28 healthy controls (HCs). FC patients randomly received 16 acupuncture (n = 40) or sham acupuncture (n = 40) sessions over 4 weeks; HCs received no treatment. The change in the proportion of patients with mean weekly complete spontaneous bowel movements (CSBMs) was considered as the primary outcome measure. Moreover, the composition and the predictive metabolic function of the gut microbiota from feceal samples were analyzed by 16S rRNA gene sequencing, while feceal SCFAs were identified via gas chromatography-mass spectrometry (GC-MS).

Results: Compared to sham acupuncture, acupuncture significantly increased the proportion of CSBM responders, and improved spontaneous bowel movements (SBMs), straining, stool consistency, and quality of life. Moreover, Sequencing of 16S rRNA genes revealed that acupuncture improved β-diversity and restored the composition of gut microbiota. Specifically, the abundance of beneficial bacteria such as g_Lactobacillus increased while that of pathogenic bacteria such as g_Pseudomonas decreased after acupuncture, which were significantly correlated with alleviated symptoms. Moreover, ten microbes including g_Coprobacter, g_Lactobacillus, and g_Eubacterium_coprostanoligenes_group might be considered acupuncture-specific microbes, and formed a stable interaction network. Additionally, GC-MS analysis indicated that acupuncture increased the content of butyrate acid in the gut, which was positively correlated with an increase in defecation frequency and a decrease in acupuncture-related pathogens. Finally, acupuncture specific-microbes including g_Coprobacter, g_Lactobacillus, g_Pseudomonas, g_Eubacterium_coprostanoligenes_group, g_Erysipelotrichaceae_UCG.003, g_Prevotellaceae_UCG.001, and g_Rolstonia could accurately predict the clinical efficacy of acupuncture (AUC = 0.918).

Conclusion: Acupuncture could effectively improve clinical symptoms in FC patients, and was associated with gut microbiota reshaping and increased butyrate acid levels. Moreover, key microbial genera such as g_Coprobacter and g_Lactobacillus was predictive of acupuncture efficacy in treating FC. Future studies are required to validate the causal relationship between key microbial genera and acupuncture clinical efficacy, and should explore further metabolic pathways for designing personalized treatment strategies.

Clinical Trial Registration:http://www.chictr.org.cn, Identifier: ChiCTR2100048831.
Comparative effect of antihypertensive drugs in improving arterial stiffness in adults with hypertension (RIGIPREV study). A network meta-analysis
Authors: Cavero-Redondo Iván , Saz-Lara Alicia , Lugones-Sánchez Cristina , Pozuelo-Carrascosa Diana P. , Gómez-Sánchez Leticia , López-Gil José Francisco , García-Ortiz Luis , Bruno Rosa Maria , Gómez-Marcos Manuel Ángel
Published: September 2023

Aims: To synthesize and evaluate the available scientific evidence on the efficacy of antihypertensive drugs on arterial stiffness in patients with hypertension by using a network meta-analysis approach.

Methods: A systematic search of the MEDLINE (via PubMed), Scopus, and Web of Science databases was conducted to identify experimental studies addressing the effect of different antihypertensive drugs on arterial stiffness parameters (pulse wave velocity [PWV] and augmentation index [AIx]) in adults with hypertension. Comparative evaluation of the effect of antihypertensive drugs was performed by conducting a standard pairwise meta-analysis and a network meta-analysis for direct and indirect comparisons between antihypertensive drugs and placebo/other antihypertensive drugs. Analyses were performed including studies of any duration and only studies longer than 6 months length.

Results: Seventy-six studies were included in the main analysis and considering only studies longer than 6 months length, thiazide diuretics, ACEIs, ARBs, the ACEI/ARB combination, the ACEI/CCB combination, and the ARB/CCB combination showed a higher effect on reducing PWV, and ACEIs and ARBs on reducing AIx.

Conclusion: Our research provides evidence that antihypertensive medications are an effective way to treat arterial stiffness in adults with hypertension. Based on our findings, patients with hypertension who have greater levels of arterial stiffness may benefit from using thiazide diuretics, ACEIs, ARBs, the ACEI/ARB combination, the ACEI/CCB combination, and the ARB/CCB combination.

Systematic Review Registration: PROSPERO (CRD42021276360).
The thickness of current sheets and implications for coronal heating
Authors: Klimchuk James A. , Leake James E. , Daldorff Lars K. S. , Johnston Craig D.
Published: September 2023

The thickness of current sheets is extremely important, especially as it relates to the onset of fast magnetic reconnection. Onset determines how much magnetic free energy can build up in a field before it is explosively released. This has implications for many phenomena on the Sun and throughout the Universe, including the heating of the solar corona. Significant effort has been devoted to the question of whether equilibrium current sheets in realistic geometries have finite or zero thickness. Using a simple force balance analysis, we show why current sheets without a guide field (2D) and with a guide field that is invariant in the guide field direction (2.5D) cannot be in equilibrium if they have both finite thickness and finite length. We then estimate the conditions under which the tension of a curved line-tied guide field can facilitate equilibrium in 3D sheets that are finite in all dimensions. Finally, we argue that some quasi-statically evolving current sheets undergoing slow stressing—e.g., when the coronal magnetic field is subjected to photospheric boundary driving—may reach a critical shear, at which point they lose equilibrium, spontaneously collapse, and reconnect. The critical shear is generally consistent with the heating requirements of solar active regions.
Platelet mitochondria, a potent immune mediator in neurological diseases
Authors: Ma Yan , Jiang Qian , Yang Bingxin , Hu Xiaoyu , Shen Gang , Shen Wei , Xu Jing
Published: September 2023

Dysfunction of the immune response is regarded as a prominent feature of neurological diseases, including neurodegenerative diseases, malignant tumors, acute neurotraumatic insult, and cerebral ischemic/hemorrhagic diseases. Platelets play a fundamental role in normal hemostasis and thrombosis. Beyond those normal functions, platelets are hyperactivated and contribute crucially to inflammation and immune responses in the central nervous system (CNS). Mitochondria are pivotal organelles in platelets and are responsible for generating most of the ATP that is used for platelet activation and aggregation (clumping). Notably, platelet mitochondria show marked morphological and functional alterations under heightened inflammatory/oxidative stimulation. Mitochondrial dysfunction not only leads to platelet damage and apoptosis but also further aggravates immune responses. Improving mitochondrial function is hopefully an effective strategy for treating neurological diseases. In this review, the authors discuss the immunomodulatory roles of platelet-derived mitochondria (PLT-mitos) in neurological diseases and summarize the neuroprotective effects of platelet mitochondria transplantation.
Dousing the flame: reviewing the mechanisms of inflammatory programming during stress-induced intrauterine growth restriction and the potential for ω-3 polyunsaturated fatty acid intervention
Authors: White Melanie R. , Yates Dustin T.
Published: September 2023

Intrauterine growth restriction (IUGR) arises when maternal stressors coincide with peak placental development, leading to placental insufficiency. When the expanding nutrient demands of the growing fetus subsequently exceed the capacity of the stunted placenta, fetal hypoxemia and hypoglycemia result. Poor fetal nutrient status stimulates greater release of inflammatory cytokines and catecholamines, which in turn lead to thrifty growth and metabolic programming that benefits fetal survival but is maladaptive after birth. Specifically, some IUGR fetal tissues develop enriched expression of inflammatory cytokine receptors and other signaling cascade components, which increases inflammatory sensitivity even when circulating inflammatory cytokines are no longer elevated after birth. Recent evidence indicates that greater inflammatory tone contributes to deficits in skeletal muscle growth and metabolism that are characteristic of IUGR offspring. These deficits underlie the metabolic dysfunction that markedly increases risk for metabolic diseases in IUGR-born individuals. The same programming mechanisms yield reduced metabolic efficiency, poor body composition, and inferior carcass quality in IUGR-born livestock. The ω-3 polyunsaturated fatty acids (PUFA) are diet-derived nutraceuticals with anti-inflammatory effects that have been used to improve conditions of chronic systemic inflammation, including intrauterine stress. In this review, we highlight the role of sustained systemic inflammation in the development of IUGR pathologies. We then discuss the potential for ω-3 PUFA supplementation to improve inflammation-mediated growth and metabolic deficits in IUGR offspring, along with potential barriers that must be considered when developing a supplementation strategy.
Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.)
Authors: Perveen Nusrat , Dinesh M. R. , Sankaran M. , Shivashankara K. S. , Ravishankar K. V. , Venugopal R. , Mir Hidayatullah
Published: September 2023

Introduction

Putative mutants were generated through gamma irradiation in the polyembryonic mango genotype Nekkare. The putative mutant progenies along with control seedlings and mother plants were evaluated by comparing the compositions and relative proportions of their major volatile compounds.

Methods

Volatile profiling was done using headspace-solid phase micro-extraction (HS SPME) method coupled with gas chromatography–mass spectrometry (GC-MS MS). Furthermore, characterisation of putative mutants and control seedlings was carried out using simple sequence repeat (SSR) markers to ascertain the genetic diversity present in the samples under study.

Results

Monoterpenes were the most abundant volatile compound in all the studied samples (ranging from 34.76% to 91.41%) out of which I-Phellandrene and cis-Ocimene formed the major fraction in mother plants (20.45%–21.86% and 16.17%–21.27%, respectively) and control seedlings (23.32%–24.95% and 18.95%–20.81%, respectively), while beta-Phellandrene was dominant in the selected putative mutant samples (2.34%–29.53%). Among sesquiterpenes, trans-Caryophyllene was detected only in the putative mutant samples (0.10%–30.18%). Grouping together of mother plants and control seedlings was seen in the cluster analysis, while the putative mutants grouped apart from them suggesting genetic diversity. Genetic distance between the mother plants and control seedlings ranged from 0.97 to 2.73, while between putative mutants, control seedlings, and mother plants, it ranged from 6.54 to 9.82. SSR-based characterisation of putative mutant seedlings showed that mutation caused variability in the treated population. This was evident from the high allelic richness ranging from 4 to 12 with a mean of 7 and a higher mean Shannon’s Information Index (1.50) of the putative mutant population.

Discussion

The study demonstrates that volatile profiling and molecular characterisation using SSR markers could be used as a tool to detect variation in a mutated population. In addition, volatile profiling can be used to validate putative mutants in polyembryonic mango genotypes where the seedlings of nucellar origin are similar to mother plants.
Plant and pathogen genomics: essential approaches for stem rust resistance gene stacks in wheat
Authors: Jost Matthias , Outram Megan A. , Dibley Kathy , Zhang Jianping , Luo Ming , Ayliffe Michael
Published: September 2023

The deployment of disease resistance genes is currently the most economical and environmentally sustainable method of crop protection. However, disease resistance genes can rapidly break down because of constant pathogen evolution, particularly when they are deployed singularly. Polygenic resistance is, therefore, considered the most durable, but combining and maintaining these genes by breeding is a laborious process as effective genes are usually unlinked. The deployment of polygenic resistance with single-locus inheritance is a promising innovation that overcomes these difficulties while enhancing resistance durability. Because of major advances in genomic technologies, increasing numbers of plant resistance genes have been cloned, enabling the development of resistance transgene stacks (RTGSs) that encode multiple genes all located at a single genetic locus. Gene stacks encoding five stem rust resistance genes have now been developed in transgenic wheat and offer both breeding simplicity and potential resistance durability. The development of similar genomic resources in phytopathogens has advanced effector gene isolation and, in some instances, enabled functional validation of individual resistance genes in RTGS. Here, the wheat stem rust pathosystem is used as an illustrative example of how host and pathogen genomic advances have been instrumental in the development of RTGS, which is a strategy applicable to many other agricultural crop species.