bluuebunny/crossref_metadata_2025 huggingface dataset:
PREPARANDO O ESTUDANTE DE MEDICINA PARA O ATENDIMENTO, COMUNICAÇÃO E CUIDADO DA PESSOA USUÁRIA DE SUBSTÂNCIAS PSICOATIVAS
Authors: BARBOSA DAYSE VIEIRA SANTOS , MACEDO JULIANE , SILVEIRA MARLUCE MARTINS MACHADO DA , USEVÍCIUS PRISCILA MARIA , NUNES WILSON
Published: July 2019

INTRODUÇÃO: As Diretrizes Curriculares Nacionais do Curso de Graduação em Medicina (Resolução CNE nº 314/2014) preconizam a formação geral, humanista, crítica, reflexiva e ética do graduado, visando a concretizar a comunicação com pessoas, familiares, comunidades e membros das equipes profissionais com empatia, sensibilidade e interesse. Por sua vez, a Conferência Internacional de Ensino de Comunicação em Medicina, realizada em Oxford, em 1996, recomenda, entre itens básicos necessários à formação (graduação) e ao desenvolvimento (educação continuada) do profissional médico, que o ensino deverá orientar e ajudar o estudante no esforço da comunicação centrada no paciente;  que o ensino e a avaliação da comunicação deverão proporcionar o desenvolvimento pessoal e profissional; e que as habilidades estudadas deverão ser avaliadas diretamente na prática. A disciplina “Habilidades em Comunicação” busca desenvolver a relação entre alunos e pacientes drogaditos por meio de metodologias ativas de ensino e aprendizagem, com componente reflexivo, a fim de superar as dificuldades de comunicação. OBJETIVO: Analisar processo de aprendizagem de estudantes do curso de medicina em atividades práticas de comunicação com internos e egressos de uma comunidade terapêutica. MÉTODOS: Relato das experiências de 56 alunos do quarto período do curso de medicina da UniEVANGÉLICA, frente ao desenvolvimento de três intervenções: discussão de filme sobre drogadição, visita a uma comunidade terapêutica e entrevistas com usuários e ex-usuários de substâncias psicoativas e seus familiares. Utilizou-se metodologia qualitativa, por meio da técnica hermenêutico-dialética proposta por Habermas e objetivada por Minayo (2014), para análise dos relatos das vivências dos estudantes nas três atividades propostas. RESULTADOS: Foram evidenciados impactos das três estratégias de ensino-aprendizagem na reflexão e, consequentemente, desconstrução e reconstrução de valores do discente frente à condição da pessoa em drogadição. CONCLUSÃO: Resultados reforçam relevância do ensino de habilidades em comunicação na educação médica e a utilização de práticas reflexivas que contribuem com o desenvolvimento do cuidado integral e empático à pessoa, à família e à comunidade.
ALIENAÇÃO PARENTAL NAS RELAÇÕES HOMOAFETIVAS
Authors: LIMA VALDINA JOSÉ , OLIVEIRA CÉSAR GRATÃO DE
Published: July 2019

O presente trabalho tem como objetivo analisar a alienação parental nas relações homoafetivas e seus efeitos negativos causados na criança e no adolescente; bem como a importância da família na hora de resolver os conflitos advindos da separação do casal. O abuso emocional desmedido imputado à criança pode acarretar sequelas por toda a vida. Este trabalho foi realizado por levantamento de pesquisas, familiarizando as ideias dos autores que dialogam sobre o tema da alienação parental. Por fim, uma análise na legislação brasileira e uma ênfase na lei 12.318/2010 após a sua promulgação e efetiva aplicabilidade nos dias atuais.
Pengaruh Penggunaan Lensa Kontak, Kelembapan, dan Pengetahuan Terhadap Dry Eyes Syndrome
Authors: Rahmawati Isnaini
Published: July 2019

Objective: The Effect of Contact Lenses, humidity, and knowledge on the dry eyes syndrome.Methods: The type of reserach used is observation. The dimensions of study time are cross sectional because of data collection and outcome at one time. Humidity variables were measured using thermohygrometer and users’characteristic were obtained by questionnaire. The statistical test used is multiple logistic regression to find out effect of  Contact Lenses Type, length of lens usage, humidity, and knowledge on the dry eyes syndrome.Results:  Respondents were students  Faculty of Public Health UNAIR from 2014-2017. Based on the type of lens, 90.9% of respondents used daily wear and 9.1% of respondents used overnight wear or extended wear. Based on the length of use of the lens  84.8% using a lens more than 4 hours and 15.2% less than 4 hours. Based on knowledge of 51.5% having high knowledge and 48.5% having low knowledge. Based on the humidity of the space of 54.5% the humidity level of the air conditioned lecture hall is appropriate and 45.5% is not suitable.Logisti regression test results obtained value of 4.732 Wald with a probability of 0.030, which means that there is a significant effect of moisture on the dry eyes syndrome while the user characteristics there is no significant effect. Conclusion:  Based on the results of research conducted Students of the Faculty of Public Health, there are some conclusions that most respondents use the type of daily wear lens. Respondents use contact lenses more than 4 hours and users' knowledge in the high category. Humidity of spaces in the Faculty of Public Health is 54.5% stated to be appropriate and 45.5% is declared inappropriate.
Justificaciones de profesorado de ciencias en formación inicial para la selección de actividades educativas. Influencia de una propuesta formativa
Authors: Rueda Serón José Antonio , Blanco López Ángel , España Ramos Enrique , Brero Peinado Vito Battista
Published: July 2019

Resumen:En este trabajo se presenta una investigación realizada con profesorado en formación de las especialidades de Física y Química y de Biología y Geología del Máster en Profesorado de Educación Secundaria. Se ha llevado a cabo en el contexto de una propuesta formativa centrada en las actividades de enseñanza aprendizaje y su importancia en la planificación de la enseñanza. La finalidad de esta propuesta es la mejora de la competencia docente para el análisis y la selección de actividades de enseñanza y aprendizaje. En concreto, se analizan y se comparan, mediante una metodología pretest-postest las decisiones y justificaciones que los profesores en formación inicial utilizan para la elección de una actividad educativa antes y después de la propuesta formativa. Para el análisis de las respuestas se ha elaborado un sistema de categorías a partir de las justificaciones aportadas y de la revisión de la literatura al respecto. Los resultados obtenidos muestran una mejora en la capacidad de los futuros docentes  para justificar sus decisiones, a través de un incremento en el número de justificaciones y una atención creciente a aquellas centradas en las demandas que las actividades plantean al alumnado, acorde con los objetivos de la propuesta formativa.
Quality of Live Improvement Antituberculosis Consumer
Authors: Adnan Jumasni
Published: July 2019

Antituberculosis is the most liver damage causes. Rifampicin and Isoniazide, in combination, are toxic compounds. Isoniazide and rifampicin metabolits causes lipid peroxidation. The hepatoprotective effect of rosella calyx water extract on liver damage induced with Isoniazide-rifampicin evaluated by examination of malondialdehid levels in the liver organ. 25 male wistar rats divided into 5 groups, ie group I (INH-rifampicin + rosella water extract 250 mg/kgBW), group II (INH-rifampicin + rosella water extract 125 mg/kgBW), group III (INH-rifampicin + rosella water extract 62.5 mg/kgBW),  group IV (healthy control) and group V (Isoniazide-rifampicin). MDA liver levels were analyzed after 35 days of treatments. The test results of each group are, group I has mean MDA levels 0.023912 + 0.011 mg/ml, group II 0.023526 + 0.009 mg/ml, group III 0.027168 + 0.007 mg/ml group IV 0.03437 + 0.009 mg/ml and group V 0.236846 + 0.118 mg/ml. The kruskal-wallis test showed significantly value 0.008 (p <0.05) and Post hoc Mann U whitney test showed that group V was significantly different to group I, II, III, and IV (p = 0.008) respectively, roselle extract can be used as a hepatoprotector antioxidant to improve the tuberculosis drug consumer quality of life through improved health by lowering lipid peroxidation that causes liver damage.
Sexist language of “Pelakor and Pebinor”
Authors: Mustakim Rezki Amalia Wahyuni
Published: July 2019

When in social media or everyday life rampant infidelity occurs, whether due to lifestyle, economy, and so forth. Economic demands intrigue principals to do so unnaturally. Until sometimes netizens make the acronym separate from the words of the affair, such as a beautiful interlude intact households, distractions in saturated times, a beautiful family interlude collapsed, and so forth. The perpetrators of infidelity sometimes even openly execute. Because they consider the affair is a natural thing in the present day. Infidelity sometimes happens either fiction or in a literary or non-fiction / real work. Society sometimes herded for various opinions that reflect one party. Be it both material and inmaterial matter. And this leads the author to issue words that lately often heard of the pelakor and pebinor.
Surface integral and holography
Authors: Kuyukov Vitaly
Published: July 2019

Holographic surface and integral surface
KAJIAN TINGKAT KEBISINGAN DARI AKTIVITAS PELABUHAN
Authors: Tinimbang Fahd Ligar
Published: July 2019

Indonesia adalah negara maritim. Sebab itu, pelayaran menjadi sektor penting dalam menyokong kehidupan sosial, ekonomi, pemerintahan, pertahanan dan keamanan, budaya, serta lainnya. Untuk mendukung hal tersebut tentunya membutuhkan prasarana berupa pelabuhan. Pelabuhan memiliki beberapa aktivitas yang kompleks, ini menjadikannya sumber pencemaran yang penting terutama ketika pelabuhan dilokalisasi sangat dekat dengan daerah permukiman, karena dalam kesehariannya, pelabuhan juga mempunyai hubungan erat dengan warga sekitar yang mendiami kawasan pelabuhan. Salah satu faktor pencemarnya adalah kebisingan. Maka dari itu pengelolaan dan pengembangan pelabuhan yang tidak tepat dan tidak berkelanjutan berarti dapat mengganggu kualitas hidup warga dan pekerja. Menurut Menteri Tenaga Kerja RI no 13 tahun 2011, Kebisingan adalah semua suara yang tidak dikehendaki yang bersumber dari alat-alat proses produksi dan/atau alat-alat kerja yang pada tingkat tertentu dapat menimbulkan gangguan pendengaran. Karya tulis ilmiah ini bertujuan untuk mengetahui besaran tingkat kebisingan akibat aktivitas pelabuhan serta mengevaluasi kebisingan di area pelabuhan dengan mengacu pada baku mutu KEP48/MENLH/11/1996. Pengukuran tingkat kebisingan pada area pelabuhan dilakukan berdasarkan Keputusan Menteri Lingkungan Hidup Nomor 48 Tahun 1996 Tentang Baku Tingkat Kebisingan. Pengukuran dilakukan dengan mencatat data setiap 5 detik selama 10 menit di beberapa titik. Waktu pengukuran dilakukan selama aktivitas 24 jam pada siang dan malam hari, yaitu tingkat aktivitas paling tinggi selama 16 jam pada selang waktu 06.00-22.00 WIB dan aktivitas malam hari pada selang 22.00-06.00. Melalui karya ilmiah ini diharapkan pencemaran berupa kebisingan dapat direduksi dengan melakukan upaya-upaya pencegahan.
FAKTOR PENDUKUNG MENINGKATKAN PRESTASI SISWA
Authors: MASRUFA ANI
Published: July 2019

Faktor pendukung meningkatkan prestasi siswa ada dua yaitu faktor internal dan faktor eksternal. Faktor internal meliputi semangat belajar, percaya diri, dan bakat. Sedangkan faktor eksternal meliputi lingkungan keluarga maupun pergaulan
EFEKTIVITAS PENGGUNAAN BAHAN KOAGULAN DALAM PROSES PERENCANAAN BANGUNAN PENGOLAHAN AIR MINUM
Authors: Lestari Septyn Anggun
Published: July 2019

Pengolahan air minum diperlukan untuk menghasilkan air minum yang sesuai dengan Permenkes RI No. 492 Tahun 2010 Tentang Persyaratan Kualitas Air Minum agar dapat dikonsumsi oleh masyarakat. Instalasi Pengolahan Air Minum terdiri dari beberapa unit seperti intake, koagulasi, flokulasi, sedimentasi, filtrasi dan desinfeksi. Salah satu proses utama dala Instalasi Pengolahan Air Minum adalah proses koagulasi yang berfungsi untuk mengikat partikel – partikel di dalam air dengan menambahkan koagulan. Pemberian dosis koagulan yang tidak tepat dapat merusak kualitas air. Metode yang digunakan untuk mencari dosis optimum minimum adalah jartest. Proses koagulasi bisa terhambat jika tingkat kekeruhan terlalu rendah atau terlalu tinggi. Untuk itu perlu ditentukan dosis optimum pemakaian koagulan pada kondisi kekeruhan air baku yang berbeda. Salah satu bahan koagulan yang dapat digunakan dalam poses penjernihan air adalah Pollyaluminium Chloride (PAC) dan Alumnium Sulfat. Kedua bahan koagulan ini paling banyak dipakai dan mudah diperolah di pasar. Kisaran pH dengan koagulan Aluminium Sulfat berkisar antara 5,5-7,5. Tujuan karya ilmiah ini adalah untuk mendapatkan jenis dan dosis koagulan yang tepat dalam proses pengolahan air baku menjadi air minum. Karya ilmiah ini diharapkan dapat memberikan informasi ilmiah tentang penggunaan jenis koagulan yang efektif dan efisien dalam memperbaiki kualitas air baku.
Individual differences in inhibitory control: A latent variable analysis
Authors: Gärtner Anne , Strobel Alexander
Published: July 2019

Inhibitory control represents a central component of executive functions and focuses on the ability to actively inhibit or delay a dominant response to achieve a goal. Although various tasks exist to measure inhibitory control, correlations between these tasks are rather small, partly because of the task impurity problem. To alleviate this problem, a latent variable approach has been previously applied and two closely related to each other yet separable functions have been identified: prepotent response inhibition and resistance to distractor interference. The goal of our study was a) to replicate the proposed structure of inhibitory control and b) to extend previous literature by additionally accounting for speed-accuracy trade-offs, thereby potentially increasing explained variance in the investigated latent factors. To this end, 190 participants completed six inhibitory control tasks (stroop task, antisaccade task, stop-signal task, Eriksen flanker task, shape-matching task, word-naming task). Analyses were conducted using standard scores (response times) as well as inverse efficiency scores (combining response times and error rates). In line with previous studies, we found generally low zero-order correlations between the six tasks. By applying confirmatory factor analysis using standard reaction time difference scores, we were not able to replicate a satisfactory model with good fit to the data. By using inverse efficiency scores, a single latent variable response–distractor inhibition emerged that resembled previous literature, but only four out of six tasks demonstrated significant factor loadings. Our results highlight the difficulty in finding robust inter-correlations between commonly used inhibitory control tasks, even when applying a latent variable analysis and accounting for speed-accuracy trade-offs.
A Survey of Enhanced Publication Features of China’s Science and Technology Research Journals in 2018
Authors: Fang Qing , Zhan Lijuan , Peng Wei
Published: July 2019

Enhanced publication features that extend information access, add variety to presentation formats, and improve reader comprehension have become a part of China’s academic journals in science and technology (sci-tech for short) in recent years. We sought to determine the degree of their adoption. By surveying 472 Chinese sci-tech journals, we found that 102 of these journals had enhanced publication features. Thus less than a quarter of Chinese sci-tech journals in our sample had adopted enhanced publication at the time of our survey. Moreover, the enhancing features of the 102 journals were mostly simple ones, which did not depend on authors providing supplemental content. More of these 102 journals are published by scholarly associations than by other types of publishers, and the disciplinary distribution of the journals was imbalanced, with the discipline of medicine and health having the lowest percentage of journals with enhanced features among those disciplines with such journals. This finding is out of step with large international publishers, whose medical journals frequently have features of enhanced publication. These results reveal a gap between the practices of large international publishers and those of China’s publishers when it comes to adopting enhanced publishing features for sci-tech journals.
Effect of nanoparticle reinforcement on mechanical properties and erosion–corrosion behavior of cast aluminum
Authors: Irfan Osama M. , Irfan Mohammad A. , Almufadi Fahad A.
Published: July 2019

The effect of mixing alumina (Al2O3) nanoparticles in pure aluminum has been investigated, with a characterization of the mechanical properties and erosion–corrosion (E–C) behavior. Specimens of pure aluminum and Al with nanoparticles added as reinforcement have been produced by casting. Uniform dispersion of nanoparticles in the molten aluminum matrix has been achieved by mechanical stirring. Mechanical properties such as yield strength, ultimate strength, modulus of elasticity, and hardness of the new composites have been measured. E–C tests have been conducted to investigate the effect of the nanoparticle additives on the weight loss and surface properties of the new composites. Scanning electron microscopy, electron backscatter diffraction, and energy dispersive spectrometry have been employed to analyze, characterize, and compare pure aluminum and the composites. Al–Al2O3 nanocomposites have been obtained successfully and with a reasonable distribution by adding 1 wt.-% of alumina nanoparticles (100 nm or 20 nm in size) to molten aluminum. The alumina nanoparticles have increased the yield strength and hardness of the new composites when compared to pure aluminum. Enhancement in E–C resistance is also observed under various conditions.
Medicina veterinária de mega catástrofes no Brasil: história e despreparo repetidos
Authors: Souza Marcos
Published: July 2019

The Veterinary Medicine of Catastrophes is still very incipient in our country and, for that reason, all the information generated about pre and post disaster interventions must be published in order to more efficiently mitigate possible future calamities. With the intensification of human intervention on ecosystems, many risks are created for ecological health. In general, such situations occur abruptly due to an adverse phenomenon that is foreseeable or not, for humans, animals and the environment, which require immediate actions to mitigate the negative effects. It becomes increasingly clear the need to specialize and recognize the area of expertise of professionals in the "Veterinary Medicine Tactical, Disasters and Catastrophes", since the procedures associated with such situations are distinct from the other areas of veterinary medicine and have an emergency character.
Le lien faible coopératif
Authors: Lecoutre Marc , Lièvre Pascal
Published: July 2019

Malgré un voisinage conceptuel qui semble aller de soi, les recherches sur la coopération et sur les réseaux se sont développées à l’écart l’une de l’autre. Les auteurs proposent de les relier en considérant la mobilisation d’un lien social comme un processus d’engagement à la coopération. L’articulation du programme de Granovetter sur les notions de lien faible et lien fort avec l’approche coopérative duale de Dameron permet de distinguer le lien faible qui ne coopère pas de celui comportant un potentiel coopératif. C’est un enjeu pour un manager en quête de ressource quand il mobilise des liens sociaux dans et hors de son organisation.
Entreprendre en commun(s)
Authors: Aufrère Laura , Eynaud Philippe , Gauthier Olivier , Vercher-Chaptal Corinne
Published: July 2019

Les littératures sur l’entrepreneuriat et les communs sont généralement distinctes. Pourtant, on observe sur le terrain des initiatives qui conjuguent une démarche entrepreneuriale et une volonté de créer des communs. L’article1 renseigne ce rapprochement en mobilisant les perspectives processuelles qui se développent dans le champ de l’entrepreneuriat et dans celui des communs. Ce cadre permet d’analyser, via une étude ethnographique, le processus d’émergence d’un supermarché coopératif organisé en commun(s). Les résultats montrent que le rapprochement entre la démarche entrepreneuriale et la création de communs repose sur une communauté organisante à même de mobiliser une pluralité de principes d’échanges.
Effects of Olive Leaf Extract on Prevention of Molecular, Histopathological, and Enzymatic Changes in Chicken Carbon Tetrachloride-Induced Liver Damage
Authors: Vahidi-Eyrisofla Nazila , Hojati Vida , Yazdian Mohammad-Reza , Zendehdel Morteza , Shajiee Hooman
Published: July 2019

Background: Today, the use of additives such as antibiotics and growth hormones that increase production efficiency in breeding broiler chickens has become inevitable. However, the use of such additives and antibiotics associated with side effects such as liver damage. Oxidative stress occurs due to an imbalance between oxidants and antioxidants. Studies have shown that olive leaves have an antioxidant effect on free radicals. This study was to evaluate the possible effect of olive leaf extract on carbon tetrachloride (CCL4)-induced liver damage (molecular and tissue) and changes of enzymes in chickens. Materials and Methods: A total of 50 chickens were used and classified into5 groups. Treatment groups received 0.5, 1, and 1.5 mg/kg of the olive leaf extract from day 21 of the experiment. Two control groupshealthy and poisoneddid not receive any extract. On the day 35 of the experiment, 1cc of CCL4 was dissolved with olive oil and injected intraperitoneally into the experimental and poisoned control groups. Blood and liver tissue sampling were performed. Results: The histopathology results showed that at high doses of olive leaf extract, the cells and vessels were regularly curable, and sinusoids were healthy. The expression of B-cell lymphoma 2 (BCL2) increased, and that of BH3 interacting domain death agonist (BID)decreased. Enzymatic tests, including serum glutamic-oxaloacetic transaminase, serum glutamic-pyruvic transaminase, alkaline phosphatase, gamma-glutamyl transpeptidase, showed a reduction in BID expression in the experimental group compared with the control group(P<0.005). Conclusion: We concluded that olive leaf extract boosts the BCL2—an antiapoptotic gene—and reduces BID—an apoptosis gene—in the liver of chicken. It prevents the liver cells from disintegrating and destroys sinusoids and liver blood vessels. The high doses of the olive leaf extract caused liver resistance to CCL4 toxicity in chicken. [GMJ.2019;8:e1204]
Migracje Słowa Bożego, czyli obrona wartości Wulgaty według Franciszka Titelmansa.
Authors: Mantyk Tomasz Karol
Published: July 2019

Nowe tłumaczenie Nowego Testamentu, dokonane bezpośrednio z greki, zostało opublikowane przez Erazma z Rotterdamu w 1516 r. stając się ważnym punktem odniesienia dla badań nad tekstem Biblii. Dzieło Erazma zdawało się podważać dotychczasowe tłumaczenie, Wulgatę, i wywołało wiele polemik. Jednym z krytyków Erazma był młody franciszkanin z Lovanium, Franciszek Titelmans. W swoim Prologus Apologeticus zarzucał on Erazmowi motywowane pychą poszukiwanie próżnej chwały i bronił autorytetu obowiązującej od stuleci Wulgaty. Sednem jego argumentacji było przeświadczenie o migracjach Słowa Bożego. Objawienie przenosiło się z języka do języka pod ścisłym nadzorem Boskiej Opatrzności, co gwarantowało wierność przekazu. Tym samym, kwestia tłumaczenia nie była li tylko sprawą filologii, lecz wymagała natchnienia Bożego. Argumentacja Titelmansa znalazła częściowe odzwierciedlenie w dekretach Soboru Trydenckiego poświęconych Biblii. Co prawda szczegóły jego wywodu zupełnie nie przystają do stanu współczesnych badań nad Biblią, to jednak pewne ogólne założenia obecne w jego polemice z Erazmem są zaskakująco bliskie bieżącym debatom, czyniąc ową kontrowersję sprzed blisko pięciuset lat ważnym zagadnieniem badawczym.
Optimalisasi Personal Awareness Anak Usia Dini Melalui “The 7 Habits”
Authors: Wahyuningtyas Dessy Putri
Published: July 2019

The purpose of this research is to explain the process and result of applying The 7 Habits method that can optimization personal awareness in group B TK Negeri Pembina Surabaya. This study uses the Kemmis and Mc Taggart procedures which consists of four stages: planning, action, observation, and reflection. The data analysis technique consisted of quantitative and qualitative data analysis. Quantitative data analysis is presented in the form of tables and graphs. While qualitative data analysis using technique according to Miles and Huberman which consists of data reduction, data display, and verification collected through personal awareness instruments, interviews, field notes, and documentation. After the first cycle of action, the average value of 68,47%. Then after second cycle of action, the average value increase to 84,17%. It can be concluded that by using the 7 Habits method can improve personal awareness in children.
Regulation of PGC-1α Isoform Expression in Skeletal Muscles
Authors: Popov D. V. , Lysenko E. A. , Kuzmin I. V. , Vinogradova O. L. , Grigoriev A. I.
Published: July 2019

The coactivator PGC-1 is the key regulator of mitochondrial biogenesis in skeletal muscle. Skeletal muscle expresses several PGC-1 isoforms. This review covers the functional role of PGC-1 isoforms and the regulation of their exercise-associated expression in skeletal muscle. The patterns of PGC-1 mRNA expression may markedly differ at rest and after muscle activity. Different signaling pathways are activated by different physiological stimuli, which regulate the expression of the PGC-1 gene from the canonical and alternative promoters: expression from a canonical (proximal) promoter is regulated by activation of the AMPK; expression from an alternative promoter, via a 2-adrenergic receptor. All transcripts from both promoters are subject to alternative splicing. As a result, truncated isoforms that possess different properties are translated: truncated isoforms are more stable and predominantly activate angiogenesis, whereas full-length isoforms manly regulate mitochondrial biogenesis. The existence of several isoforms partially explains the broad-spectrum function of this protein and allows the organism to adapt to different physiological stimuli. Regulation of the PGC-1 gene expression by different signaling pathways provides ample opportunity for pharmacological influence on the expression of this gene. Those opportunities might be important for the treatment and prevention of various diseases, such as metabolic syndrome and diabetes mellitus. Elucidation of the regulatory mechanisms of the PGC-1 gene expression and their functional role may provide an opportunity to control the expression of different isoforms through exercise and/or pharmacological intervention.
Cancer Invasion: Patterns and Mechanisms
Authors: Krakhmal N. V. , Zavyalova M. V. , Denisov E. V. , Vtorushin S. V. , Perelmuter V. M.
Published: July 2019

Cancer invasion and the ability of malignant tumor cells for directed migration and metastasis have remained a focus of research for many years. Numerous studies have confirmed the existence of two main patterns of cancer cell invasion: collective cell migration and individual cell migration, by which tumor cells overcome barriers of the extracellular matrix and spread into surrounding tissues. Each pattern of cell migration displays specific morphological features and the biochemical/molecular genetic mechanisms underlying cell migration. Two types of migrating tumor cells, mesenchymal (fibroblast-like) and amoeboid, are observed in each pattern of cancer cell invasion. This review describes the key differences between the variants of cancer cell migration, the role of epithelial-mesenchymal, collective-amoeboid, mesenchymal-amoeboid, and amoeboid-mesenchymal transitions, as well as the significance of different tumor factors and stromal molecules in tumor invasion. The data and facts collected are essential to the understanding of how the patterns of cancer cell invasion are related to cancer progression and therapy efficacy. Convincing evidence is provided that morphological manifestations of the invasion patterns are characterized by a variety of tissue (tumor) structures. The results of our own studies are presented to show the association of breast cancer progression with intratumoral morphological heterogeneity, which most likely reflects the types of cancer cell migration and results from different activities of cell adhesion molecules in tumor cells of distinct morphological structures.
NeuN As a Neuronal Nuclear Antigen and Neuron Differentiation Marker
Authors: Gusel’nikova V. V. , Korzhevskiy D. E.
Published: July 2019

The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. Monoclonal antibodies to the NeuN protein have been actively used in the immunohistochemical research of neuronal differentiation to assess the functional state of neurons in norm and pathology for more than 20 years. Recently, NeuN antibodies have begun to be applied in the differential morphological diagnosis of cancer. However, the structure of the protein, which can be revealed by antibodies to NeuN, remained unknown until recently, and the functions of the protein are still not fully clear. In the present mini-review, data on NeuN accumulated so far are summarized and analyzed. Data on the structure and properties of the protein, its isoforms, intracellular localization, and hypothesized functions are reported. The application field of immunocytochemical detection of NeuN in scientific and clinical studies, as well as the difficulties in the interpretation of the obtained experimental data and their possible causes, is described in details.
Search for Human Lactate Dehydrogenase A Inhibitors Using Structure-Based Modeling
Authors: Nilov D. K. , Prokhorova E. A. , Švedas1 V. K.
Published: July 2019

The human lactate dehydrogenase isoform A plays an important role in the anaerobic metabolism of tumour cells and therefore constitutes an attractive target in the oncology field. Full-atom models of lactate dehydrogenase A (in complex with NADH and in the apo form) have been generated to enable structure-based design of novel inhibitors competing with pyruvate and NADH. The structural criteria for the selection of potential inhibitors were established, and virtual screening of a library of low-molecular-weight compounds was performed. A potential inhibitor, STK381370, was identified whose docking pose was stabilized through additional interactions with the loop 96-111 providing for the transition from the open to the closed conformation.
Specific Depletion of Myelin-Reactive B Cells via BCR-Targeting
Authors: Stepanov A. V. , Belogurov A. A. , Kothapalli P. , Shamborant O. G. , Knorre V. D. , Telegin G. B. , Ovsepyan A. A. , Ponomarenko N. A , Deyev S. M. , Kaveri S. V. , Gabibov A. G.
Published: July 2019

B cells play a crucial role in the development and pathogenesis of systemic and organ-specific autoimmune diseases. Autoreactive B cells not only produce antibodies, but also secrete pro-inflammatory cytokines and present specific autoantigens to T cells. The treatment of autoimmune diseases via the elimination of the majority of B cells using the monoclonal anti-CD19/20 antibody (Rituximab) causes systemic side effects and, thus, requires a major revision. Therapeutic intervention directed towards selective elimination of pathogenic autoreactive B cells has the potential to become a universal approach to the treatment of various autoimmune abnormalities. Here, we developed a recombinant immunotoxin based on the immunodominant peptide of the myelin basic protein (MBP), fused to the antibody Fc domain. We showed that the obtained immunotoxin provides selective in vivo elimination of autoreactive B cells in mice with experimental autoimmune encephalomyelitis. The proposed conception may be further used for the development of new therapeutics for a targeted treatment of multiple sclerosis and other autoimmune disorders.
Detection of T-Cadherin Expression in Mouse Embryos
Authors: Rubina К. А. , Smutova V. А. , Semenova М. L. , Poliakov А. А. , Gerety S. , Wilkinson D. , Surkova Е. I. , Semina Е. V. , Sysoeva V. Yu , Tkachuk V. А.
Published: July 2019

The aim of the present study was to evaluate T-cadherin expression at the early developmental stages of the mouse embryo. Using in situ hybridization and immunofluorescent staining of whole embryos in combination with confocal microscopy, we found that T-cadherin expression is detected in the developing brain, starting with the E8.75 stage, and in the heart, starting with the E11.5 stage. These data suggest a possible involvement of T-cadherin in the formation of blood vessels during embryogenesis.
Clonal Rearrangements and Malignant Clones in Peripheral T-cell Lymphoma
Authors: Sidorova Yu. V. , Chernova N. G. , Ryzhikova N. V. , Smirnova S. Yu. , Sinicina M. N. , Vinogradova Yu. E. , Julhakyan H. L. , Kovrigina A. M , Zvonkov E. E. , Sudarikov A. Б.
Published: July 2019

Aim: To assess the feasibility and informative value of T-cell clonality testing in peripheral T-cell lymphoma (PTCL). Patients and methods: Biopsies of involved sites, blood, and bone marrow samples from 30 PTCL patients are included in the study. Rearranged TCRG and TCRB gene fragments were PCR-amplified according to the BIOMED-2 protocol and analyzed by capillary electrophoresis on ABI PRISM 3130 (Applied Biosystems). Results: TCRG and TCRB gene clonality assay was valuable in confirming diagnosis in 97% of PTCL patients. T-cell clonality assay performed on blood or bone marrow samples reaffirmed lymphoma in 93% of cases, whereas morphological methods were informative in 73% of cases only. We observed multiple TCRG and TCRB gene rearrangements, loss of certain clones in the course of the disease, as well as acquisition of new clones in 63% of PTCL cases, which can be attributed to the genetic instability of the tumor. Conclusion: TCRG and TCRB gene clonality assay is beneficial for the diagnosis of PTCL. However, the presence of multiple clonal rearrangements should be considered. Clonal evolution in PTCL, particularly acquisition of new clones, should not be treated as a second tumor. Multiple TCRG and TCRB gene rearrangements may interfere with minimal residual disease monitoring in PTCL.
Regulatory Elements in Vectors for Efficient Generation of Cell Lines Producing Target Proteins
Authors: Maksimenko O. G. , Gasanov N. B. , Georgiev P. G.
Published: July 2019

To date, there has been an increasing number of drugs produced in mammalian cell cultures. In order to enhance the expression level and stability of target recombinant proteins in cell cultures, various regulatory elements with poorly studied mechanisms of action are used. In this review, we summarize and discuss the potential mechanisms of action of such regulatory elements.
Additivity of the Stabilization Effect of Single Amino Acid Substitutions in Triple Mutants of Recombinant Formate Dehydrogenase from the Soybean Glycine max
Authors: Alekseeva A. A. , Kargov I. S. , Kleimenov S. Yu. , Savin S. S. , Tishkov V I.
Published: July 2019

Recently, we demonstrated that the amino acid substitutions Ala267Met and Ala267Met/Ile272Val (Alekseeva et al., Biochemistry, 2012), Phe290Asp, Phe290Asn and Phe290Ser (Alekseeva et al., Prot. Eng. Des. Select, 2012) in recombinant formate dehydrogenase from soya Glycine max (SoyFDH) lead to a significant (up to 30-100 times) increase in the thermal stability of the enzyme. The substitutions Phe290Asp, Phe290Asn and Phe290Ser were introduced into double mutant SoyFDH Ala267Met/Ile272Val by site-directed mutagenesis. Combinations of three substitutions did not lead to a noticeable change in the catalytic properties of the mutant enzymes. The stability of the resultant triple mutants was studied through thermal inactivation kinetics and differential scanning calorimetry. The thermal stability of the new mutant SoyFDHs was shown to be much higher than that of their precursors. The stability of the best mutant SoyFDH Ala267Met/Ile272Val/Phe290Asp turned out to be comparable to that of the most stable wild-type formate dehydrogenases from other sources. The results obtained with both methods indicate a great synergistic contribution of individual amino acid substitutions to the common stabilization effect.
Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein
Authors: Bogdanov I. V. , Finkina E. I. , Balandin S. V. , Melnikova D. N. , Stukacheva E. A. , Ovchinnikova T. V.
Published: July 2019

The recombinant isoforms Lc-LTP1 and Lc-LTP3 of the lentil lipid transfer protein were overexpressed in E. coli cells. It was confirmed that both proteins are stabilized by four disulfide bonds and characterized by a high proportion of the -helical structure. It was found that Lc-LTP1 and Lc-LTP3 possess antimicrobial activity and can bind fatty acids. Both isoforms have the ability to bind specific IgE from sera of patients with food allergies, which recognize similar epitopes of the major peach allergen Pru p 3. Both isoforms were shown to have immunological properties similar to those of other plant allergenic LTPs, but Lc-LTP3 displayed a less pronounced immunoreactivity.
事业单位的人事档案的信息化管理
Authors: 南 浩
Published: July 2019

事业单位作为政府部门职能与工作效果昀直接的展示社会服务组织,其中的人事档案管理过程不仅是整体工作过程中的关键环节,还是保证事业单位人员信息安全的重要步骤。随着社会的不断发展,人事档案的数量以及类别也越来越多,因此需要利用先进的信息化管理模式提高档案管理效率。本文简述了人事档案管理的重要作用,并就其中出现的问题提出了对应的优化措施。
社会分层视角下我国实现教育公平的思考
Authors: 刘 睿 , 孙 光懿
Published: July 2019

教育是公民的基本权利,教育公平是社会公平的重要基础。当前,在社会分层趋势不断扩大的情况下,教育公平问题更加备受关注。笔者首先阐释了社会分层与教育公平的关系,接下来从不同视角详细分析了社会分层对教育公平产生的差异,通过国外促进教育公平的经验借鉴,提出了社会分层视角下我国实现教育公平的思考。
Tres nociones acerca de la ciudad como lo otro: altopía, ciudad accidental y vernáculo urbano
Authors: Vielma Cabruja Jose Ignacio
Published: July 2019

Se presenta una discusión argumentada acerca de la condición otra de la ciudad contemporánea desde una perspectiva físico-espacial. Esto a partir de tres imágenes correlacionadas con tres nociones desarrolladas previamente por el autor. Primero, altopía (1999-2010), que describe los otros lugares como un residuo físico y conceptual del desarrollo urbano actual. Luego, ciudad accidental (2009), que da cuenta de la distancia creciente entre los proyectos urbanos y la experiencia resultante en el marco de la instrumentalidad urbana actual. Por último, vernáculo urbano (2017), que describe las soluciones con las cuales las personas atienden sus problemas, y establecen nuevas tradiciones y relaciones sociales características del espacio urbano. Metodológicamente, se propone como un análisis descriptivo del registro físico-espacial, por lo que la discusión parte de la experiencia presente en las fotografías, y en relación con autores clásicos y contemporáneos. Se determina que las tres nociones revisadas implican distintas escalas y niveles de profundización en la experiencia urbana comprendida como experiencia otra, resultando de interés el rol participativo de las personas en la determinación de esta experiencia de la alteridad, como se termina registrando en la noción de vernáculo urbano.
La inversión en innovación tecnológica: la clave de américa latina frente a escenario global postcrisis
Authors: Curillo Gonzalez Enma Elizabeth , Espinoza Espinoza Wendy Nathaly , Estrada Carrera Flor María Lorena
Published: July 2019

En este artículo se expone el origen de la crisis económica mundial de 2008, y cómo esta a su vez, reconfiguró la globalización y el comercio internacional. Se establece la génesis de la crisis, su repercusión en una nueva de fragmentación de la cadena de producción mundial en cuyo trayecto se genera valor mediante la inversión en innovación tecnológica. Se destaca cómo la débil institucionalidad de la economía internacional no contribuye a: (a) reducir la brecha financiera y tecnológica entre países industrializados y periféricos, tal como lo son las naciones latinoamericanas, (b) ni ayuda a desmontar las barreras proteccionistas de las economías fuertes, y (c) mucho menos coadyuva a enfrentar la tendencia mundial a limitar el financiamiento extranjero. Ante este escenario complejo y competitivo -donde adicionalmente está presente la competencia e influencia de los BRICS- se explica cómo a América Latina no le queda otra opción que invertir en innovación tecnológica para superar la heterogeneidad estructural de la producción de sus países, incorporar valor en las cadenas productivas mundiales y conquistar o crear nichos de mercado. Para tal efecto, es menester trabajar de forma concertada en los bloques regionales para establecer estrategias conjuntas frente el escenario del comercio internacional.
El Microcurrículo de inglés y su influencia en el desarrollo de las competencias comunicativas.
Authors: Salas Castelo Edison Marcelo , Inca Chunata Nanci Margarita , Rojas Yumisaca Wilson Gonzalo , Basantes Arias Elsa Amalia , Escobar Murillo María Guadalupe
Published: July 2019

El objetivo de la presente investigación fue realizar un diagnóstico para determinar si el Microcurrículo influye en el desarrollo de las competencias comunicativas en el aprendizaje del idioma inglés en los estudiantes de la Escuela de Ingeniería en Ecoturismo, Facultad de Recursos Naturales de la Escuela Superior Politécnica de Chimborazo, en la ciudad de Riobamba. Esta investigación se enmarco en la teoría constructivista debido a que el docente junto con los estudiantes son protagonistas de construir su propio conocimiento de acuerdo a sus necesidades y contexto que viven. La investigación fue de campo, documental y bibliográfica porque se investigó en el lugar de los hechos a través de la recolección de información y con la asociación de las variables de estudio, las mismas que permitieron determinar cómo influye el diseño microcurricular de inglés sobre el desarrollo de las competencias comunicativas de los estudiantes. Los resultados obtenidos fueron tabulados, analizados, interpretados y sometidos a la prueba estadística Chi cuadrado; prueba que permitió comprobar las hipótesis y obteniendo como resultado que el Microcurrículo si influye en el desarrollo de las competencias comunicativas, ya que dentro de los contenidos no existen suficientes actividades prácticas tendientes a facilitar el mejor desarrollo de las destrezas. Es así, que se ha propuesto rediseñar la planificación microcurricular e implementar el uso de manuales con actividades prácticas tendientes a desarrollar las habilidades básicas del idioma inglés: un texto con actividades para estudiantes y un texto guía con sus respuestas para el docente.
MENGGAGAS PERENCANAAN KURIKULUM MENUJU SEKOLAH UNGGUL
Authors: Saufi Akhmad , Hambali Hambali
Published: July 2019

Excellent school is identical with quality school. Many people assume that to become a Excellent school must have large financial capital because the availability of facilities and capable resources require big costs. This statement is not entirely true, because besides facilities and human resources involved in educational activities, the most important part of education is the curriculum. This paper aims to find out how curriculum planning ideas can be applied in excellent school. The curriculum as a medium for achieving educational goals also serves as the main guideline for implementing learning activities. Therefore, the design of the curriculum in education must be positioned as a platform for schools to develop education. The results of the author's analysis describe that the curriculum planning framework in excellent schools is developed from local-regional to national level. Curriculum planning process must pay attention to the holistic approach and eliminate hierarchies in decision making. The socio-cultural approach is also essential by involving competent human resources including teachers as curriculum implementers.
Validation of Anatomical Sites for the Measurement of Infrared Body Surface Temperature Variation in Response to Handling and Transport
Authors: Rocha Luiene M. , Devillers Nicolas , Maldague Xavier , Kabemba Fidèle Z. , Fleuret Julien , Guay Fréderic , Faucitano Luigi
Published: July 2019

This study aimed at validating the anatomical sites for the measurement of infrared (IR) body surface temperature as a tool to monitor the pigs’ response to handling and transport stress. The selected anatomical sites were the neck (infrared neck temperature—IRNT), rump (infrared rump temperature—IRRT), orbital (infrared orbital temperature—IROT) and behind ears (infrared behind ears temperature—IRBET) regions. A total of 120 pigs were handled from the finishing pen to the loading dock through a handling test course. Two handling types (gentle vs. rough) and number of laps (1 vs. 3) were applied according to a 2 × 2 factorial design. After loading, pigs were transported for 40 min and returned to their home pens. Animal behavior, heart rate, rectal temperature and salivary cortisol concentration were measured for validation. Increased IR body temperature, heart rate and salivary cortisol levels were observed in response to rough handling and longer distance walk (P < 0.05 for all). The greatest correlations were found between IROT and IRBET temperatures and salivary cortisol concentration at the end of the handling test (r = 0.49 and r = 0.50, respectively; P < 0.001 for both). Therefore, IR pig’s head surface temperature may be useful for a comprehensive assessment of the physiological response to handling and transport stress.
Detection and Monitoring of Tunneling-Induced Riverbed Deformation Using GPS and BeiDou: A Case Study
Authors: Guo Wen , Wang Guoquan , Bao Yan , Li Pengfei , Zhang Mingju , Gong Qiuming , Li Rui , Gao Yang , Zhao Ruibin , Shen Shuilong
Published: July 2019

Shield tunneling under rivers often requires monitoring riverbed deformations in near real-time. However, it is challenging to measure riverbed deformation with conventional survey techniques. This study introduces a comprehensive method that uses the Global Positioning System (GPS) of the USA and the BeiDou navigation satellite system (BeiDou) of China to monitor riverbed deformation during the construction of twin tunnels beneath the Hutuo River in Shijiazhuang, China. A semi-permanent GPS network with one base station outside the river and six rover stations within the river was established for conducting near real-time and long-term monitoring. The distances between the base and the rover antennas are within two kilometers. The network was continuously operating for eight months from April to December 2018. The method is comprised of three components: (1) Monitoring the stability of the base station using precise point positioning (PPP) method, a stable regional reference frame, and a seasonal ground deformation model; (2) monitoring the relative positions of rover stations using the carrier-phase double-difference (DD) positioning method in near real-time; and (3) detecting abrupt and gradual displacements at both base and rover stations using an automated change point detection algorithm. The method is able to detect abrupt positional-changes as minor as five millimeters in near real-time and gradual positional-changes at a couple of millimeters per day within a week. The method has the flexibility of concurrent processing different GPS and BeiDou data sessions (e.g., every 15 minutes, 30 minutes, one hour, one day) for diffident monitoring purposes. This study indicates that BeiDou observations can also achieve few-millimeter-accuracy for measuring displacements. Parallel processing GPS and BeiDou observations can improve the reliability of near real-time structural deformation monitoring and minimize false alerts. The method introduced in this article can be applied to other urban areas for near real-time and long-term structural health monitoring.