bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediksi Harga Komoditas Pangan di Indonesia Menggunakan Backpropagation
Authors: Farisi Olief Ilmandira Ratu , Jannah Nurul , Insania Ribki
Published: February 2023

Pangan merupakan kebutuhan paling mendasar bagi sumber daya manusia. Ketahanan pangan suatu bangsa dapat diukur dari tersedianya pangan dalam jumlah dan kualitas yang cukup. Pemerintah memiliki tanggung jawab dan pengendalian dalam ketersediaan bahan pangan pokok. Oleh karena itu, perlu kebijakan dari pemerintah untuk menentukan harga eceran tertinggi, salah satu caranya adalah memprediksi harga sedini mungkin dengan menggunakan Backpropagation. Pada penelitan ini, diuji beberapa arsitektur yang untuk menentukan kestabilan model yang digunakan. Hasil uji coba menunjukkan bahwa arsitektur 10-10-1 cukup stabil digunakan untuk memprediksi berbagai jenis data harga komoditas pangan yang berbeda karakteristiknya dengan tingkat akurasi di atas 90%. Rata-rata akurasi dari prediksi harga komoditas pangan di Indonesia yaitu sebesar 96,448% dengan waktu rata-rata adalah 6,8495 detik.
Prediksi Pemesanan Produk Untuk Membangun Permintaan Pasar Dengan Menggunakan Neuro Fuzzy
Authors: . Mochammad Faid , Khairi Ahmad , Hasyim Fuadz
Published: February 2023

Keuntungan terbesar berasal dari penjualan terbesar. Jika jumlah produk yang ditawarkan oleh perusahaan lebih sedikit dari jumlah permintaan, maka perusahaan kehilangan kesempatan untuk memperoleh keuntungan yang maksimal, begitu pula sebaliknya. Oleh karena itu, merencanakan jumlah pembelian produk untuk gudang sangatlah penting. Untuk untuk memenuhi permintaan pasar secara tepat dan dalam jumlah yang tepat. Faktor-faktor yang perlu dipertimbangkan saat menentukan harga pembelian, persediaan dan volume penjualan. Survei ini dilakukan dengan menggunakan metode fuzzy Neoro. Hasil penerapan metode fuzzy Neoro terbukti dapat membantu perusahaan dalam mengambil keputusan untuk menentukan pembelian produk sehingga persediaan memenuhi permintaan pasar.
A ‘large family’ in ‘circumstances of interest and excitement’: British Troops and the Occupation of France, 1815–1818
Authors: Haynes Christine
Published: February 2023

In the aftermath of the Napoleonic Wars, British troops participated in not one, but two multinational occupations of France. However, although they were central to the military, social, and political history of Great Britain in this period, these occupations have attracted little scholarly attention. Drawing on a wide range of primary and secondary sources, this article examines the experiences of British troops who participated in the occupations of 1814 and 1815–1818. While these experiences were mixed and varied with class and regional origin, in general they reinforced both traditional regimental and emerging national identity, while also enabling British troops to reconcile with – and even learn politically from – their former enemies. Following these occupations, the troops who had participated often found themselves on opposite sides of the political conflicts in Britain and its empire. For those who remained in the military, many of them found keeping the peace in France to be an easier assignment than policing radical unrest in Great Britain and its empire, which relied on similar tactics.
study Desain & Implementasi BUSINESS INTELLIGENCE pada suatu maskapai penerbangan (laporan penelitian magang MSIB)
Authors: Guruh Sukmo  , Sulung Rahmawan Wira Ghani
Published: February 2023

Satisfying service is a priority for airlines so airlines compete to create comfort. In addition, flights also affect external matters because the airline needs to minimize errors which often occur. To overcome this, it is necessary to monitor the performance of airlines. One method used is the application of a business intelligence system which can provide reporting dashboards that can provide interactive data analysis information. The amount of data from flight traffic stored in the data warehouse becomes very useful if managed properly to get various kinds of hidden insights. With the implementation of business intelligence, the data can be utilized so it becomes a tool in making decisions for the development and improvement of air traffic and the impact of air traffic. The object of this research used raw data from public data. This research method used the Business Intelligence Roadmap (BIR). The results of the study made 3 dashboards: the first dashboard showed a pattern which was almost the same as the total delay data in 3 consecutive years. This was due to a high proportion of weather conditions in America. The second dashboard depicted a positive correlation between airplane visits and economic growth. The third dashboard indicated the number of flights that were frequently used by passengers as a standard reference that the need for and comfort for aircraft facilities because the quality of service provided by the airline was very important for consumers who used flight services.
Umbrot tengd Bárðarbungu og Grímsvötnum á 19. öld
Authors: Brandsdóttir Bryndís , Pálsson Finnur
Published: February 2023

Frásagnir í annálum, gömlum bréfum, dagbókum, dagblöðum sem og munnmælasögur af eldsumbrotum í ríki Vatnajökuls hafa ýtt undir rannsóknir á eldfjallasögu landsins, frá skráningum Jónasar Hallgrímssonar á eldgosum og jarðskjálftum og eldfjallasögu Þorvaldar Thoroddsen, til nútímans. Í bókinni Vötnin Stríð rakti Sigurður Þórarinsson sögu Grímsvatnagosa og Skeiðarárhlaupa á grundvelli þeirrar þekkingar sem þá var til staðar. Með tilkomu fyrstu gervitunglamyndanna af Vatnajökli árið 1973 áttuðu menn sig á að Bárðarbunga er megineldstöð með ísfylltri öskju (Sigurður Þórarinsson og fl. 1974). Í Gjálpargosinu árið 1996 varð mönnum ljóst að eldvirkni norðan Grímsvatna gæti allt eins stafað af kvikugangi til suðurs frá Bárðarbungu sem til norðurs frá Grímsvötnum. Reynslan af Gjálpargosinu og jökulhlaupinu sem því var samfara kallar á endurtúlkun eldri gagna bæði hvað varðar aðgreiningu og tengsl á milli eldstöðvakerfa sem og samanburð á jökulhlaupum fyrri tíma. Hér er fjallað um eldsumbrot í og við Bárðarbungu á tímabilinu 1838–1903 ásamt samantekt á eldvirkni innan vatnasviðs Grímsvatna sem marka má af stærri Skeiðarárhlaupum (Sigurður Þórarinsson, 1974; Helgi Björnsson, 2009). Stuðst er við frásagnir úr dagblöðum frá þessum tíma sem aðgengileg eru á vefslóðinni timarit.is.
Klifin Herðubreið (Climbing Herðubreið)
Authors: Sæmundsson Kristján
Published: February 2023

Í „Ódáðahrauni“ Ólafs Jónssonar er fyrirsögn eins kaflans „Saga Ódáðahrauns“. Þar er rakin saga rannsókna og leiðangra um Hraunið. Einn af þeim sem þar kemur við sögu er William Lee Howard. Um hann hefur Ólafur á að byggja frásögn hans sjálfs í New York Tribune 19. október 1881. Þar greinir Howard blaðamanni frá helstu afrekum sínum á Íslandi, en flest er þar með slíkum ýkjum að Ólafur líkir honum við Vellygna-Bjarna. Ólafur greinir frá helstu ýkjunum, en sleppir hinu sem meira vit er í og þá var ofarlega í huga fólks, svo sem harðindunum 1881 og Vesturheimsferðum mormóna. Mesta afrek sitt taldi Lee Howard vera að klífa Herðubreið fyrstur manna og fá sönnun þess að hún væri eldfjall. Aðferð hans við klifrið er hins vegar í meira lagi skáldleg. Ólafur Jónsson á bágt með að trúa því að Lee Howard hafi getað greint rétt frá byggingu Herðubreiðar nema hann hafi klifið hana, enda sé lýsing hans réttari og greinilegri í verulegum atriðum en Þorvalds Thoroddsen. Til fróðleiks fylgir hér blaðagreinin úr New York Tribune.
Ferðir til Azoreyja árin 1989 til 1994 (Geodetic research in the Azores 1989–1994)
Authors: Ólafsson Halldór
Published: February 2023

Í upphafi níunda áratugar síðustu aldar voru sett á laggir samtök eldfjallastöðva um víða veröld. Þessi samtök nefndust World Organization of Volcano Observatories og voru skammstöfuð WOVO. Íslendingum veittist sá heiður að forstöðumaður Norrænu eldfjallastöðvarinnar, Guðmundur Ernir Sigvaldason jarðefnafræðingur, var kjörinn fyrsti forseti þessara samtaka. Sá gjörningur varð til þess að samvinna jókst umtalsvert milli Norrænu eldfjallastöðvarinnar og annara stofnana með sama hlutverk. Má þar nefna stofnanir og háskóla á Ítalíu, í Portúgal, Frakklandi, Bandaríkjunum, Japan og ýmsum Suður-Ameríkuríkjum. ...
Panel Data Analysis of the Effects of Female Labor Force Participation on Profit Rates
Authors: ELVEREN Adem Yavuz , DAVİS Cameron , BUDD Josh
Published: February 2023

Using a novel dataset, this brief note investigates the effect of female labor force participation (FLFP) on three important variables, profit shares, profit rates, and capital-output ratio, covering a very large set of countries (i.e., 130) over a recent time period of 1990-2019. This paper shows that while FLFP increases profit share in high-income countries, it reduces in middle-income countries. However, for both middle- and high-income countries, FLFP cannot prevent the overall tendency in profit rates to decline caused by a consistent decline in output-capital ratio.
O USO DE GAMES: UMA PRÁTICA DISCUTIDA COMO INOVADORA NA EDUCAÇÃO 5.0
Authors: Possato Alvaro Bubola , Zamoner Zuleika , Monteiro Patricia Ortiz , Querido de Oliveira Chamon Edna Maria
Published: February 2023

O processo de ensino e aprendizagem demanda constante inovação, técnica e metodológica, tendo em vista que a internet, seus recursos e ferramentas pautam a vida de muitas pessoas na sociedade contemporânea. Este estudo realizou uma revisão de literatura sobre a gamificação e a Educação 5.0 a partir da análise comparativa de desenhos produzidos por alunos de uma escola pública sobre como se sentem com o uso de games nas disciplinas da escola. Gamificar a educação tem se mostrado como uma estratégia de ensino eficaz para atrair a atenção dos estudantes, os estimulando à autonomia e à competitividade colaborativa, qualidades preconizadas na formação dos estudantes. O acesso à internet e às ferramentas tecnológicas para toda a população ainda se mostra como um desafio a ser superado, com a criação de políticas públicas que garantam acessibilidades a todos.
EDITORIAL DOSSIÊ TEMÁTICO - INOVAÇÕES
Authors: Camarini Gladis , Monteiro Patricia Ortiz , Santos André , Triani Felipe , Chamon Edna Maria Querido de Oliveira
Published: February 2023

Em consonância com o escopo da Interação – Revista de Ensino, Pesquisa e Extensão, o dossiê “Inovações: Desafios para a gestão, a academia, o mercado e a sociedade” amplia as reflexões multidisciplinares sobre as experiências e perspectivas da inovação em diferentes áreas de conhecimento e como influencia e melhora a qualidade de vida da sociedade.
Impact of technology use on workforce performance and information access in the construction industry
Authors: Ramadan Bassam , Nassereddine Hala , Taylor Timothy R. B. , Goodrum Paul
Published: February 2023

For decades, labor shortage has been a consistent struggle in the construction industry. At the same time, technological innovations have played a central role in the growth and development of an increasingly diverse construction industry. Existing research indicates that technological adoption is crucial for enhancing project productivity. Despite the importance and potential benefits of technology use, no research has yet studied how technology use on-site impact the worker’s performance and the difficulty of the process to access needed information. The objective of this paper is to analyze and understand the impact of on-site technology use on 1) the self-evaluated performance record (including safety, attendance, quality, productivity, and initiative), and 2) the difficulty of the process to access information, of construction workers and frontline supervisors in the construction industry. To achieve the research objective, 2,780 construction craft workers and frontline supervisors were surveyed using an online questionnaire. The survey participants were asked to self-evaluate 1) their work personal performance record (including safety, attendance, quality, productivity, and initiative), and 2) the difficulty of the process to receive or get access to information. The participants were additionally asked to specify whether different listed information technologies, material technologies, and equipment technologies are used on site. The collected data was then analyzed. Key findings indicate that the on-site use of several technologies had statistically significant impact in increased worker performance and information access. This study contributes to the body of knowledge by empirically quantifying the impact of on-site technology use on worker performance and information access in the construction industry.
Barriers to and enablers of type 2 diabetes screening among women with prior gestational diabetes: A qualitative study applying the Theoretical Domains Framework
Authors: Lake Amelia J. , Williams Amelia , Neven Adriana C. H. , Boyle Jacqueline A. , Dunbar James A. , Hendrieckx Christel , Morrison Melinda , O’Reilly Sharleen L. , Teede Helena , Speight Jane
Published: February 2023

Introduction

Women with previous gestational diabetes mellitus (GDM) are at increased risk of type 2 diabetes (T2D). Guidelines recommend postnatal diabetes screening (oral glucose tolerance test or HbA1c) typically 6-12 weeks after birth, with screening maintained at regular intervals thereafter. Despite this, around half of women are not screened, representing a critical missed opportunity for early identification of prediabetes or type 2 diabetes. While policy and practice-level recommendations are comprehensive, those at the personal-level primarily focus on increasing screening knowledge and risk perception, potentially missing other influential behavioral determinants. We aimed to identify modifiable, personal-level factors impacting postpartum type 2 diabetes screening among Australian women with prior gestational diabetes and recommend intervention functions and behavior change techniques to underpin intervention content.

Research design and methods

Semi-structured interviews with participants recruited via Australia’s National Gestational Diabetes Register, using a guide based on the Theoretical Domains Framework (TDF). Using an inductive-deductive approach, we coded data to TDF domains. We used established criteria to identify ‘important’ domains which we then mapped to the Capability, Opportunity, Motivation–Behavior (COM-B) model.

Results

Nineteen women participated: 34 ± 4 years, 19 ± 4 months postpartum, 63% Australian-born, 90% metropolitan, 58% screened for T2D according to guidelines. Eight TDF domains were identified: ‘knowledge’, ‘memory, attention, and decision-making processes’, ‘environmental context and resources’, ‘social influences’, ‘emotion’, ‘beliefs about consequences’, ‘social role and identity’, and ‘beliefs about capabilities’. Study strengths include a methodologically rigorous design; limitations include low recruitment and homogenous sample.

Conclusions

This study identified numerous modifiable barriers and enablers to postpartum T2D screening for women with prior GDM. By mapping to the COM-B, we identified intervention functions and behavior change techniques to underpin intervention content. These findings provide a valuable evidence base for developing messaging and interventions that target the behavioral determinants most likely to optimize T2D screening uptake among women with prior GDM.
Skin infectome of patients with a tick bite history
Authors: Zhang Jie , Zheng Yuan-Chun , Chu Yan-Li , Cui Xiao-Ming , Wei Ran , Bian Cai , Liu Hong-Bo , Yao Nan-Nan , Jiang Rui-Ruo , Huo Qiu-Bo , Yuan Ting-Ting , Li Jie , Zhao Lin , Li Lian-Feng , Wang Qian , Wei Wei , Zhu Jin-Guo , Chen Mei-Chao , Gao Yan , Wang Fei , Ye Jin-Ling , Song Ju-Liang , Jiang Jia-Fu , Lam Tommy Tsan-Yuk , Ni Xue-Bing , Jia Na
Published: February 2023

Introduction

Ticks are the most important obligate blood-feeding vectors of human pathogens. With the advance of high-throughput sequencing, more and more bacterial community and virome in tick has been reported, which seems to pose a great threat to people.

Methods

A total of 14 skin specimens collected from tick-bite patients with mild to severe symptoms were analyzed through meta-transcriptomic sequencings.

Results

Four bacteria genera were both detected in the skins and ticks, including Pseudomonas, Acinetobacter, Corynebacterium and Propionibacterium, and three tick-associated viruses, Jingmen tick virus (JMTV), Bole tick virus 4 (BLTV4) and Deer tick mononegavirales-like virus (DTMV) were identified in the skin samples. Except of known pathogens such as pathogenic rickettsia, Coxiella burnetii and JMTV, we suggest Roseomonas cervicalis and BLTV4 as potential new agents amplified in the skins and then disseminated into the blood. As early as 1 day after a tick-bite, these pathogens can transmit to skins and at most four ones can co-infect in skins.

Discussion

Advances in sequencing technologies have revealed that the diversity of tick microbiome and virome goes far beyond our previous understanding. This report not only identifies three new potential pathogens in humans but also shows that the skin barrier is vital in preventing horizontal transmissions of tick-associated bacteria or virus communities to the host. It is the first research on patients’ skin infectome after a tick bite and demonstrates that more attention should be paid to the cutaneous response to prevent tick-borne illness.
Periodontal disease is associated with the risk of cardiovascular disease independent of sex: A meta-analysis
Authors: Leng Yurong , Hu Qinwen , Ling Qin , Yao Xiongda , Liu Menglu , Chen Jiawei , Yan Zhiwei , Dai Qun
Published: February 2023

Objectives

Studies have established a link between periodontal disease and cardiovascular disease (CVD), but it is unclear whether there is a sex difference in their association.

Methods

The PubMed, Embase, and Cochrane databases were searched until June, 21 2022. Cardiovascular outcomes included any CVD, myocardial infarction (MI), coronary heart disease (CHD), or stroke. Studies reported the prevalence of CVD in patients with periodontal disease and the relationship between periodontal disease and CVD. The study is registered with PROSPERO (CRD42022333663). The level of evidence and recommendations is assessed by the Grading of Recommendations for Assessment, Development and Evaluation (GRADE).

Results

Twenty-six studies were included. In patients with periodontal disease, the prevalence of CVD was 7.2% [9 studies; 95% confidence interval (CI): 2.7–13.6%], and prevalence for CHD, hypertension, stroke, and heart failure was 6.6, 25.3, 1, and 1.1%, respectively. There was a significant association between periodontal disease and CVD in men [odds ratio (OR) = 1.22; 95% CI: 1.12–1.34] and women (OR = 1.11; 95% CI: 1.05–1.17), with no significant sex difference (P > 0.05).

Conclusion

Cardiovascular disease is relatively common in patients with periodontal disease, and an increased risk of CVD is associated with periodontal disease independent of sex. Interventions targeting periodontal disease may be beneficial for CVD.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022333663.
Lack of STAT1 co-operative DNA binding protects against adverse cardiac remodelling in acute myocardial infarction
Authors: Doudin Asmma , Riebeling Theresa , Staab Julia , Menon Priyanka Rajeev , Lühder Fred , Wirths Oliver , Vinkemeier Uwe , Ivetic Aleksandar , Meyer Thomas
Published: February 2023

In this study, we addressed the functional significance of co-operative DNA binding of the cytokine-driven transcription factor STAT1 (signal transducer and activator of transcription 1) in an experimental murine model of acute myocardial infarction (MI). STAT1 knock-in mice expressing a phenylalanine-to-alanine substitution at position 77 in the STAT1 amino-terminal domain were examined for the early clinical effects produced by ligation of the left anterior descending coronary artery (LAD), an established model for MI. The F77A mutation has been previously reported to disrupt amino-terminal interactions between adjacent STAT1 dimers resulting in impaired tetramerization and defective co-operative binding on DNA, while leaving other protein functions unaffected. Our results demonstrate that a loss of STAT1 tetramer stabilization improves survival of adult male mice and ameliorates left ventricular dysfunction in female mice, as determined echocardiographically by an increased ejection fraction and a reduced left intra-ventricular diameter. We found that the ratio of STAT3 to STAT1 protein level was higher in the infarcted tissue in knock-in mice as compared to wild-type (WT) mice, which was accompanied by an enhanced infiltration of immune cells in the infarcted area, as determined by histology. Additionally, RNA sequencing of the infarcted tissue 24 h after LAD ligation revealed an upregulation of inflammatory genes in the knock-in mice, as compared to their WT littermates. Concomitantly, genes involved in oxidative phosphorylation and other metabolic pathways showed a significantly more pronounced downregulation in the infarcted tissue from STAT1F77A/F77A mice than in WT animals. Based on these results, we propose that dysfunctional STAT1 signalling owing to a lack of oligomerisation results in a compensatory increase in STAT3 expression and promotes early infiltration of immune cells in the infarcted area, which has beneficial effects on left ventricular remodelling in early MI following LAD ligation.
Eugenol modulates the NOD1-NF-κB signaling pathway via targeting NF-κB protein in triple-negative breast cancer cells
Authors: Shi Xiaoyu , Zhang Weiwei , Bao Xiao , Liu Xiaozhu , Yang Ming , Yin Chengliang
Published: February 2023

Background

The most aggressive subtype of breast cancer, triple-negative breast cancer (TNBC), has a worse prognosis and a higher probability of relapse since there is a narrow range of treatment options. Identifying and testing potential therapeutic targets for the treatment of TNBC is of high priority.

Methods

Using a transcriptional signature of triple-negative breast cancer collected from Gene Expression Omnibus (GEO), CMap was utilized to reposition compounds for the treatment of TNBC. CCK8 and colony formation experiments were performed to detect the effect of the candidate drug on the proliferation of TNBC cells. Meanwhile, transwell and wound healing assay were implemented to detect cell metastasis change caused by the candidate drug. Moreover, the proteomic approach was presently ongoing to evaluate the underlying mechanism of the candidate drug in TNBC. Furthermore, drug affinity responsive target stability (DARTS) coupled with LC-MS/MS was carried out to explore the potential drug target candidate in TNBC cells.

Results

We found that the most widely used medication, eugenol, reduced the growth and metastasis of TNBC cells. According to the underlying mechanism revealed by proteomics, eugenol could inhibit TNBC cell proliferation and metastasis via the NOD1-NF-κB signaling pathway. DARTS experiment further revealed that eugenol may bind to NF-κB in TNBC cells.

Concludes

Our findings pointed out that eugenol was a potential candidate drug for the treatment of TNBC.
MSC-CSMC: A multi-objective semi-supervised clustering algorithm based on constraints selection and multi-source constraints for gene expression data
Authors: Wang Zeyuan , Gu Hong , Zhao Minghui , Li Dan , Wang Jia
Published: February 2023

Many clustering techniques have been proposed to group genes based on gene expression data. Among these methods, semi-supervised clustering techniques aim to improve clustering performance by incorporating supervisory information in the form of pairwise constraints. However, noisy constraints inevitably exist in the constraint set obtained on the practical unlabeled dataset, which degenerates the performance of semi-supervised clustering. Moreover, multiple information sources are not integrated into multi-source constraints to improve clustering quality. To this end, the research proposes a new multi-objective semi-supervised clustering algorithm based on constraints selection and multi-source constraints (MSC-CSMC) for unlabeled gene expression data. The proposed method first uses the gene expression data and the gene ontology (GO) that describes gene annotation information to form multi-source constraints. Then, the multi-source constraints are applied to the clustering by improving the constraint violation penalty weight in the semi-supervised clustering objective function. Furthermore, the constraints selection and cluster prototypes are put into the multi-objective evolutionary framework by adopting a mixed chromosome encoding strategy, which can select pairwise constraints suitable for clustering tasks through synergistic optimization to reduce the negative influence of noisy constraints. The proposed MSC-CSMC algorithm is testified using five benchmark gene expression datasets, and the results show that the proposed algorithm achieves superior performance.
A novel approach to analyze the association characteristics between post-spliced introns and their corresponding mRNA
Authors: Bo Suling , Sun Qiuying , Ning Pengfei , Yuan Ningping , Weng Yujie , Liang Ying , Wang Huitao , Lu Zhanyuan , Li Zhongxian , Zhao Xiaoqing
Published: February 2023

Studies have shown that post-spliced introns promote cell survival when nutrients are scarce, and intron loss/gain can influence many stages of mRNA metabolism. However, few approaches are currently available to study the correlation between intron sequences and their corresponding mature mRNA sequences. Here, based on the results of the improved Smith-Waterman local alignment-based algorithm method (SW method) and binding free energy weighted local alignment algorithm method (BFE method), the optimal matched segments between introns and their corresponding mature mRNAs in Caenorhabditis elegans (C.elegans) and their relative matching frequency (RF) distributions were obtained. The results showed that although the distributions of relative matching frequencies on mRNAs obtained by the BFE method were similar to the SW method, the interaction intensity in 5’and 3’untranslated regions (UTRs) regions was weaker than the SW method. The RF distributions in the exon-exon junction regions were comparable, the effects of long and short introns on mRNA and on the five functional sites with BFE method were similar to the SW method. However, the interaction intensity in 5’and 3’UTR regions with BFE method was weaker than with SW method. Although the matching rate and length distribution shape of the optimal matched fragment were consistent with the SW method, an increase in length was observed. The matching rates and the length of the optimal matched fragments were mainly in the range of 60%–80% and 20-30bp, respectively. Although we found that there were still matching preferences in the 5’and 3’UTR regions of the mRNAs with BFE, the matching intensities were significantly lower than the matching intensities between introns and their corresponding mRNAs with SW method. Overall, our findings suggest that the interaction between introns and mRNAs results from synergism among different types of sequences during the evolutionary process.
Quantitative analysis of polyphenolic compounds in two different cultivars of marigold (Tagetes erecta L.) using high-performance thin-layer chromatography
Authors: Mir Rayees Ahmad , Irshad Saba , Argal Surendra , Agarwal Rajiv Mohan , Khatoon Sayyada
Published: February 2023

Marigold (Tagetes erecta L.), a popular ornamental plant of the family Asteraceae, is commonly cultivated in many countries, including India, for its decorative flowers. The plants grow easily in a variety of soil and climatic conditions and have been reported to damage the nematode population of soil and control indirectly harmful microbes. High-performance thin-layer chromatography (HPTLC) was utilized in the present study, with a view to identify some important biologically active compounds in the flowers and leaves of two cultivars of marigold, Pusa Narangi Gainda (PNG) and Pusa Basanti Gainda (PBG). Quantitative analyses were carried out using silica gel thin-layer chromatography (TLC) plates and toluene–ethyl acetate–formic acid (T-E-F) (13:11:2 v/v/v) as the mobile phase used. Bands of gallic acid, caffeic acid, quercetin, p-coumaric acid, and kaempferol were observed. The results revealed a greater number of compounds in leaves than in flowers, and that the cultivar PNG accumulated a greater number of compounds than PBG. Gallic acid was found in leaves and flowers of both cultivars; however, it was found maximum in the flowers of cultivar PBG. Caffeic acid and quercetin were detected in the leaves of both cultivars, whereas p-coumaric acid was detected only in the leaves and kaempferol only in the flowers of cultivar PNG. The information generated in this report may be meaningfully used for the furtherance of research on marigolds as a natural source of antioxidants, insecticides, herbicides, etc.
Biologically important areas II for cetaceans in U.S. and adjacent waters - Arctic region
Authors: Clarke Janet T. , Ferguson Megan C. , Brower Amelia A. , Fujioka Ei , Deland Sarah
Published: February 2023

We delineated and scored Biologically Important Areas (BIAs) in the Arctic region. The Arctic region extends from the Bering Strait to the Chukchi Sea, Beaufort Sea, Amundsen Gulf, and Viscount Melville Sound. This NOAA-led effort uses structured elicitation principles to build upon the first version of NOAA BIAs (BIA I) for cetaceans. In addition to narratives, maps, and metadata tables, BIA II products incorporated a scoring and labeling system to improve their utility and interpretability. BIAs are compilations of the best available science and have no inherent regulatory authority. They have been used by NOAA, other federal agencies, and the public to support marine spatial planning and marine mammal impact assessments, and to inform the development of conservation measures for cetaceans. Supporting evidence for Arctic BIA II came from data derived from aerial-, land-, and vessel-based surveys; satellite telemetry; passive acoustic monitoring; Indigenous knowledge; photo-identification; aboriginal subsistence harvests, including catch and sighting locations and stomach contents; and prey studies. BIAs were identified for bowhead (Balaena mysticetus), gray (Eschrichtius robustus), humpback (Megaptera novaeangliae), fin (Balaenoptera physalus), and beluga (Delphinapterus leucas) whales. In total, 44 BIAs were delineated and scored for the Arctic, including 12 reproduction, 24 feeding, and 8 migration BIAs. BIAs were identified in all months except January-March. Fifteen candidate areas did not have sufficient information to delineate as BIAs and were added to a watch list for future consideration in the BIA process. Some BIAs were transboundary between the Arctic region and the Aleutian Islands-Bering Sea region. Several BIAs were transnational, extending into territorial waters of Russia (in the Chukchi Sea) and Canada (in the Beaufort Sea), and a few BIAs were delineated in international waters.
Managing human activity and marine mammals: A biologically based, relativistic risk assessment framework
Authors: Southall Brandon L. , Tollit Dominic , Amaral Jennifer , Clark Christopher W. , Ellison William T.
Published: February 2023

Presented here is a broadly applicable, transparent, repeatable analytical framework for assessing relative risk of anthropogenic disturbances on marine vertebrates, with the emphasis on the sound generating aspects of the activity. The objectives are to provide managers and action-proponents tools with which to objectively evaluate drivers of potential biological risk, to identify data gaps that limit assessment, and to identify actionable measures to reduce risk. Current regulatory assessments of how human activities (particularly those that produce sound) influence the likelihood of marine mammal behavioral responses and potential injury, rely principally on generalized characterizations of exposure and effect using simple, threshold-based criteria. While this is relatively straightforward in regulatory applications, this approach fails to adequately address realistic site and seasonal scenarios, other potential stressors, and scalable outcome probabilities. The risk assessment presented here is primarily based on a common and broad understanding of the spatial-temporal-spectral intersections of animals and anthropogenic activities, and specific examples of its application to hypothetical offshore wind farms are given. The resulting species- and activity-specific framework parses risk into two discrete factors: a population’s innate ‘vulnerability’ (potential degree of susceptibility to disturbance) and an ‘exposure index’ (magnitude-duration severity resulting from exposure to an activity). The classic intersection of these factors and their multi-dimensional components provides a relativistic risk assessment process for realistic evaluation of specified activity contexts, sites, and schedules, convolved with species-specific seasonal presence, behavioral-ecological context, and natural history. This process is inherently scalable, allowing a relativistic means of assessing potential disturbance scenarios, tunable to animal distribution, region, context, and degrees of spatial-temporal-spectral resolution.
The second-generation real-time ecological environment prediction system for the Guangdong–Hong Kong–Marco Greater Bay Area: Model setup, validation, improvements, and online visualization
Authors: Luo Lin , Meng Zhao , Ma Weiwei , Huang Jingwen , Zheng Youchang , Feng Yang , Li Yineng , Liu Yonglin , Huang Yuanguang , Zhu Yuhang
Published: February 2023

With the rapidly growing population and socioeconomic development of the Guangdong–Hong Kong–Marco Greater Bay Area of China, inputs of diverse contaminants have rapidly increased. This poses threats to the water quality of the Pearl River Estuary (PRE) and adjacent seas. To provide valuable information to assist the governors, stakeholders, and decision-makers in tracking changes in environmental conditions, daily nowcasts and two-day forecasts from the ecological prediction system, namely the Coupled Great Bay Ecological Environmental Prediction System (CGEEPS), has been setup. These forecast systems have been built on the Coupled Ocean–Atmosphere–Wave–Sediment Transport modelling system. This comprises an atmospheric Weather Research Forecasting module and an oceanic Regional Ocean Modelling System module. Daily real-time nowcasts and 2-day forecasts of temperature, salinity, NO2 + NO3, chlorophyll, and pH are continuously available. Visualizations of the forecasts are available on a local website (http://www.gbaycarbontest.xyz:8008/). This paper describes the setup of the environmental forecasting system, evaluates model hindcast simulations from 2014 to 2018, and investigates downscaling and two-way coupling with the regional atmospheric model. The results shown that though CGEEPS lacks accuracy in predicting the absolute value for biological and biogeochemical environmental variables. It is quite informative to predict the spatio-temporal variability of ecological environmental changes associated with extreme weather events. Our study will benefit of developing real-time marine biogeochemical and ecosystem forecast tool for oceanic regions heavily impact by extreme weathers.
Acanthopagrus latus migration patterns and habitat use in Wanshan Islands, Pearl River Estuary, determined using otolith microchemical analysis
Authors: Tang Guanglong , He Zehua , Liu Yong , Xiao Yayuan , Bi Sheng , Sun Ye , Liang Jiman , Wu Peng , Wang Teng , Ye Jinyu , Li Chunhou
Published: February 2023

Introduction

The waters surrounding the Wanshan Islands are important traditional fishing grounds in China, with rich habitat types. Acanthopagrus latus is an economically important species in this area; however, the distribution of its spawning grounds and habitat use patterns remain unknown.

Methods

Thus 100 otolith samples of A. latus were collected from three geographic areas (MW: Qi’ao Island Mangrove Water Habitat; OW: Yamen Estuary Oyster Farm Water Habitat; RW: Dong’ao-Guishan Island Reef Water Habitat), and the concentrations of Sr and Ca along the shortest axis of the vertical otolith annual or lunar rings were measured to span the entire life cycle of A. latus, with the core and edge areas corresponding to environmental characteristics at birth and capture, respectively.

Results and discussion

Analysis of covariance (ANCOVA) revealed that the ratios of Sr/Ca in otolith edges of RW samples are significantly higher than those of OW and MW samples; however, both the values of Sr/Ca ratio in otolith cores collected from OW and MW are comparable with those of RW samples. Cluster analysis and non-metric multidimensional scaling (nMDS) indicated that at the juvenile stage, RW and MW individuals in the two main clusters belonged to the same cluster. There was no significant difference between the cores of the RW samples and the edges of the MW and OW samples. Therefore, the spawning area of A. latus in the Wanshan Islands is thought to have originated from low to medium-salinity waters with mangroves and oyster farm habitats in the Pearl River Estuary. A. latus from RW was found to have three distinct habitat-use patterns: 1) Marine Resident (7.2% of sampled fish) fish that remain in marine habitats for life; 2) Marine Migrant (16.4% of sampled fish) juveniles inhabit low to moderate salinity habitats and migrate to marine habitats as they grow; 3) Estuarine Visitor (76.4% of sampled fish) repeated migration between low to moderate salinity and marine habitats. This suggests widespread migration between estuarine and marine habitats throughout the ontogeny. The plasticity of this habitat use and the protection of spawning grounds should be considered in future fisheries management because A. Latus in this area has been the victim of the overexploitation of resources.
Association between dietary inflammatory index and risk of endometriosis: A population-based analysis
Authors: Liu Penglin , Maharjan Rashmi , Wang Yixiao , Zhang Yubo , Zhang Yanqin , Xu Chunyu , Geng Yuning , Miao Jinwei
Published: February 2023

Background and aims

Chronic inflammation plays a significant role in the etiology of endometriosis, which might be affected by dietary intake. This study aimed to investigate the association between dietary inflammatory index (DII) and the risk of endometriosis.

Methods

A cross-sectional analysis using data from the National Health and Nutrition Examination Survey (1999–2006) was conducted on 3,410 American participants, among whom 265 reported a diagnosis of endometriosis. DII scores were calculated based on the dietary questionnaire. The association of DII scores with endometriosis was evaluated by adjusted multivariate logistic regression analyzes, which were further investigated in the subgroups.

Results

In the fully adjusted models, the odds ratio (OR) for endometriosis participants in the highest and middle tertiles of DII scores were 1.57 [95% confidence interval (CI): 1.14–2.17] and 1.18 (95% CI: 0.84–1.65), compared to the lowest tertile (Ptrend = 0.007). In subgroup analyzes, the significant positive association between DII scores and the endometriosis risk was also observed in non-obese women (ORtertile3vs1: 1.69, 95% CI: 1.12–2.55; Ptrend = 0.012), women without diabetes (ORtertile3vs1: 1.62, 95% CI: 1.16–2.27; Ptrend = 0.005), women with hypertension (ORtertile3vs1: 2.25, 95% CI: 1.31–3.87; Ptrend = 0.003), parous women (ORtertile3vs1: 1.55, 95% CI: 1.11–2.17; Ptrend = 0.011), and women using oral contraceptives (ORtertile3vs1: 1.63, 95% CI: 1.15–2.30; Ptrend = 0.006).

Conclusion

This nationally representative study found that increased intake of the pro-inflammatory diet, as a higher DII score, was positively associated with endometriosis risk among American adults. Our results suggested anti-inflammatory dietary interventions may be promising in the prevention of endometriosis. Further prospective studies are necessary to confirm these findings.
Serological and cellular response to mRNA-SARS-CoV2 vaccine in patients with hematological lymphoid malignancies: Results of the study “Cervax”
Authors: Mohamed Sara , Lucchini Elisa , Sirianni Francesca , Porrazzo Marika , Ballotta Laura , Ballerini Mario , De Sabbata Giovanni Maria , De Bellis Eleonora , Cappuccio Ilaria , Granzotto Marilena , Toffoletto Barbara , Fortunati Ilaria , Russignan Anna , Florea Emilia Elzbieta , Torelli Lucio , Zaja Francesco
Published: February 2023

messenger RNA (mRNA)-Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) vaccines such as BNT162b2 became available in late 2020, but hematological malignancy patients (HM pts) were not evaluated in initial registration trials. We hereby report the results of a prospective, unicentric, observational study Response to COVID-19 Vaccination in hEmatological malignancies (CERVAX) developed to assess the postvaccine serological and T-cell-mediated response in a cohort of SARS-CoV2-negative HM pts vaccinated with BNT162b2. Patients with lymphomas [non-Hodgkin lymphoma (NHL) and Hodgkin lymphoma (HL)], chronic lymphocytic leukemia (CLL), and multiple myeloma (MM); off-therapy for at least 3 months; in a watch-and-wait program; or in treatment with ibrutinib, venetoclax, and lenalidomide were included. Different time points were considered to assess the serological response to the vaccine: before the second dose (T1), at 3–6–12 months after the first dose (T2–3–4, respectively). Since March 2021, 39 pts have been enrolled: 15 (38%) NHL, 12 (31%) CLL, and 12 (31%) MM. There were 13 of the 39 pts (33%) seroconverted at T1; an increase of the serological response was registered after the second dose (T2) (22/39 pts, 56%) and maintained after 6 months (22/39 pts, 56%) and 12 months (24/39 pts, 61%) from the first dose (T3–T4, respectively). Non-serological responders at T4 were 7/39 (18%): 0/15 NHL, 1/12 MM (8%), and 6/12 CLL (50%). All of them were on therapy (one lenalidomide, three ibrutinib, and three venetoclax). SARS-CoV2-reactive T-cell analysis (interferon gamma release assays) was available since June 2022 and was evaluated at 12 months (T4) from the first dose of vaccine in 31/39 pts (79%). T-cell-mediated-responders were 17/31 (55%): most of them were NHL and MM (47%, 41% and 12% for NHL, MM, and CLL, respectively). Both serological and T-cell non-responders were represented by pts on active therapy (venetoclax/ibrutinib). During the period of observation, eight (20.5%) pts developed mild SARS-CoV2 infection; no coronavirus disease 19 (COVID-19)-related deaths or hospitalizations were registered. In conclusion, in our cohort of lymphoproliferative pts receiving BNT162b2, CLL diagnosis and venetoclax/ibrutinib seem to be related with a lower humoral or T-mediated response. Nevertheless, the efficacy of mRNA vaccine in HM pts and the importance to continue the vaccine program even in non-responders after the first dose are supported in our study by demonstrating that a humoral and T-cell-mediated seroconversion should be observed even in the subsets of heavily immunocompromised pts.
Prognostic risk factors and nomogram construction for sebaceous carcinoma: A population-based analysis
Authors: Xu Wen , Le Yijun , Zhang Jianzhong
Published: February 2023

Background

Sebaceous gland carcinoma (SGC) is a rare tumor for which there are currently no effective tools to predict patient outcomes. We analyzed the clinical and pathological prognostic risk factors of sebaceous carcinoma based on population data and created a nomogram of related risk factors, which can more accurately predict the 3-, 5-, and 10-year overall survival (OS) rates of patients.

Methods

SGC patients between 2004 and 2015 were collected from the Surveillance, Epidemiology, and End Results (SEER) database and randomly assigned to training and validation cohorts. Relevant risk factors were identified by univariate and multivariate COX hazards regression methods and combined to produce a correlation nomogram. The concordance index (C-index), the area under the receiver operating characteristic (AUC) curve, and calibration plots have demonstrated the predictive power of the nomogram. Decision curve analysis (DCA) was used to measure nomograms in clinical practice.

Results

A total of 2844 eligible patients were randomly assigned to 70% of the training group (n=1990) and 30% of the validation group (n=854) in this study. The derived meaningful prognostic factors were applied to the establishment of the nomogram. The C-index for OS was 0.725 (95% CI: 0.706-0.741) in the training cohort and 0.710 (95% CI: 0.683-0.737) in the validation cohort. The AUC and calibration plots of 3-, 5-, and 10-year OS rates showed that the nomogram had good predictive power. DCA demonstrated that the nomogram constructed in this study could provide a clinical net benefit.

Conclusion

We created a novel nomogram of prognostic factors for SGC, which more accurately and comprehensively predicted 3-, 5-, and 10-year OS in SGC patients. This can help clinicians identify high-risk patients as early as possible, carry out personalized treatment, follow-up, and monitoring, and improve the survival rate of SGC patients.
Glaucoma and microglia-induced neuroinflammation
Authors: Ishikawa Makoto , Izumi Yukitoshi , Sato Kota , Sato Taimu , Zorumski Charles F. , Kunikata Hiroshi , Nakazawa Toru
Published: February 2023

Glaucoma is a multifactorial neurodegenerative disease characterized by a progressive optic neuropathy resulting in visual field defects. Elevated intraocular pressure (IOP) is the greatest risk factor for the development of glaucoma, and IOP reduction therapy is the only treatment currently available. However, there are many cases in which retinal degeneration progresses despite sufficient control of IOP. Therefore, it is important to elucidate the pathophysiology of glaucoma that is resistant to current IOP lowering therapies. Experiments using animal glaucoma models show the relationships between microglial neuroinflammatory responses and damage of retinal ganglion cells (RGCs). Inhibition of neuroinflammatory pathways associated with microglial activation appears to be neuroprotective, indicating that microglia may be an important therapeutic target for RGC protection. In this review, we will focus on microglia-induced neuroinflammation in the pathogenesis of glaucoma to offer new insights into the possibility of developing novel neuroprotective therapies targeting microglia.