bluuebunny/crossref_metadata_2025 huggingface dataset:
Perimenstrual Asthma in Adolescents: A Shared Condition in Pediatric and Gynecological Endocrinology
Authors: Calcaterra Valeria , Nappi Rossella Elena , Farolfi Andrea , Tiranini Lara , Rossi Virginia , Regalbuto Corrado , Zuccotti Gianvincenzo
Published: February 2022

Asthma is a frequent medical condition in adolescence. The worsening of the most common symptoms perimenstrually is defined as perimenstrual asthma (PMA). The cause of PMA remains unclear, but a role for hormonal milieu is plausible. Data on PMA in adolescents are limited, and its management is not fully established. We aimed to discuss the PMA phenomenon in young females from pathophysiology to preventive strategies, focusing on the relationship with the hormonal pattern. The fluctuation of estrogens at ovulation and before menstruation and the progesterone secretion during the luteal phase and its subsequent withdrawal seem to be the culprits, because the deterioration of asthma is cyclical during the luteal phase and/or during the first days of the menstrual cycle. Conventional asthma therapies are not always effective for PMA. Preventive strategies may include innovative hormonal contraception. Even a possible beneficial effect of other hormonal treatments, including estrogens, progestogens, and androgens, as well as leukotriene receptor antagonists and explorative approach using microbial-directed therapy, is considered. The underlying mechanisms, through which sex-hormone fluctuations influence asthma symptoms, represent a challenge in the clinical management of such a distressing condition. Further studies focused on young females are mandatory to promote adolescent health.
Stimulatory Effects of Extracellular Vesicles Derived from Leuconostoc holzapfelii That Exists in Human Scalp on Hair Growth in Human Follicle Dermal Papilla Cells
Authors: Yoon Yeo Cho , Ahn Beom Hee , Min Jin Woo , Lee Kyung Real , Park Sang Hoon , Kang Hee Cheol
Published: February 2022

Human hair follicle dermal papilla cells (HFDPCs) located in hair follicles (HFs) play a pivotal role in hair follicle morphogenesis, hair cycling, and hair growth. Over the past few decades, probiotic bacteria (PB) have been reported to have beneficial effects such as improved skin health, anti-obesity, and immuno-modulation for conditions including atopic dermatitis and inflammatory bowel disease (IBD). PB can secrete 50~150 nm sized extracellular vesicles (EVs) containing microbial DNA, miRNA, proteins, lipids, and cell wall components. These EVs can regulate communication between bacteria or between bacteria and their host. Although numerous biological effects of PB-EVs have been reported, the physiological roles of Leuconostoc holzapfelii (hs-Lh), which is isolated from human scalp tissue, and the extracellular vesicles derived from them (hs-LhEVs) are largely unknown. Herein, we investigated the effects of hs-LhEVs on hair growth in HFDPCs. We show that hs-LhEVs increase cell proliferation, migration, and regulate the cell cycle. Furthermore, hs-LhEVs were found to modulate the mRNA expression of hair-growth-related genes in vitro. These data demonstrate that hs-LhEVs can reduce apoptosis by modulating the cell cycle and promote hair growth by regulation via the Wnt/β-catenin signal transduction pathway.
Long-Term Durability of Robust Super-Hydrophobic Co–Ni-Based Coatings Produced by Electrochemical Deposition
Authors: Wang Shuqiang , Xue Yanpeng , Xue Yanyan , Lv Cunjing , Jin Ying
Published: February 2022

The long-term durability for two kinds of Co–Ni-based robust coatings, the Co–Ni super-hydrophobic (Co–Ni SHPB) coating and Co–Ni/WC super-hydrophobic (Co–Ni/WC SHPB) coating, was analyzed through an immersion test in 3.5 wt.% NaCl solution. The evolution of their surface properties was characterized by scanning electron microscope (SEM) images, energy-dispersive spectrometry (EDS), a wettability measurement and X-ray photoelectron spectrometer (XPS), and the evolution of anti-corrosion mechanisms was evaluated with electrochemical measurements. The results show that as-prepared two kinds of robust coatings display a good long-term durability, with the Co–Ni SHPB coating and Co–Ni/WC SHPB coating losing their super-hydrophobicity after being immersed for more than 10 days. Additionally, both kinds of coatings present efficient corrosion protection even after long-term immersion.
Electric Fields Enhance Ice Formation from Water Vapor by Decreasing the Nucleation Energy Barrier
Authors: Santos Leandra P. , da Silva Douglas S. , Galembeck André , Galembeck Fernando
Published: February 2022

Video images of ice formation from moist air under temperature and electric potential gradients reveal that ambient electricity enhances ice production rates while changing the habit of ice particles formed under low supersaturation. The crystals formed under an electric field are needles and dendrites instead of the isometric ice particles obtained within a Faraday cage. Both a non-classical mechanism and classical nucleation theory independently explain the observed mutual feedback between ice formation and its electrification. The elongated shapes result from electrostatic repulsion at the crystal surfaces, opposing the attractive intermolecular forces and thus lowering the ice-air interfacial tension. The video images allow for the estimation of ice particle dimensions, weight, and speed within the electric field. Feeding this data on standard equations from electrostatics shows that the ice surface charge density attains 0.62–1.25 × 10−6 C·m−2, corresponding to 73–147 kV·m−1 potential gradients, reaching the range measured within thunderstorms. The present findings contribute to a better understanding of natural and industrial processes involving water phase change by acknowledging the presence and effects of the pervasive electric fields in the ambient environment.
Crystal Plasticity Modeling of Grey Cast Irons under Tension, Compression and Fatigue Loadings
Authors: Balobanov Viacheslav , Lindroos Matti , Andersson Tom , Laukkanen Anssi
Published: February 2022

The study of the micromechanical performance of materials is important in explaining their macrostructural behavior, such as fracture and fatigue. This paper is aimed, among other things, at reducing the deficiency of microstructural models of grey cast irons in the literature. For this purpose, a numerical modeling approach based on the crystal plasticity (CP) theory is used. Both synthetic models and models based on scanning electron microscope (SEM) electron backscatter diffraction (EBSD) imaging finite element are utilized. For the metal phase, a CP model for body-centered cubic (BCC) crystals is adopted. A cleavage damage model is introduced as a strain-like variable; it accounts for crack closure in a smeared manner as the load reverses, which is especially important for fatigue modeling. A temperature dependence is included in some material parameters. The graphite phase is modeled using the CP model for hexagonal close-packed (HCP) crystal and has a significant difference in tensile and compressive behavior, which determines a similar macro-level behavior for cast iron. The numerical simulation results are compared with experimental tensile and compression tests at different temperatures, as well as with fatigue experiments. The comparison revealed a good performance of the modeling approach.
Insulin Past, Present, and Future: 100 Years from Leonard Thompson
Authors: Brink Stuart J.
Published: February 2022

Before the discovery of insulin and the critical role of the pancreas vis-à-vis diabetes mellitus pathophysiology, childhood diabetes or what we now call type 1 or autoimmune diabetes mellitus was almost universally fatal. In limited-resource countries (LRC) around the world, this remains sadly true because of the expense and unavailability of medical care, medical information, and/or medications. In 1889, Minkowski and Mering identified the pancreas as the likely source of the problem in pancreatectomized dog experiments, and Langerhans, working with Virchow, identified the islands of pancreatic tissue now named after Langerhans as the likely source of the problem. Prior to that, Cawley, Boucherdat, Zuelzer, Gley, de Meyer, Schafer, Scott, Kleiner, and Paulescu all worked on this problem with varying results until Banting, Best, MacLeod, and Collip in Toronto in 1921 successfully treated pancreatectomized dogs with an alcohol-based pancreatic extract and then were the first to do the same with children and adults with diabetes, starting with Leonard Thompson in early 1922. Urinary and blood glucose levels were reduced, and clinical symptoms decreased concurrently. The magnificent medical historical work by Professor Michael Bliss, also from Toronto, as well as an excellent US NPR Television documentary, describes the trials and tribulations of this event that culminated in the “fastest Nobel Prize” awarded. Progressive biopharmaceutical advances have modified insulin from pigs and cows and then genetically engineered insulin to work much faster and also much slower to provide more modernized ways of providing insulin. Insulin pens then replaced vial and syringe administration, and then insulin pumps coupled with continuous blood glucose sensors have made delivery more physiologic in addition to more attention paid to nutrition advice, education, and psychosocial support around the world. Programs to assist delivery of expensive insulin to LRC administered by Insulin for Life, Life for a Child (LFAC), Changing Diabetes in Children (CDIC) coupled with support by ISPAD (International Society for Pediatric and Adolescent Diabetes) have continued to make such advances available thorough wonderful philanthropy in insulin manufacturers and manufacturers of blood glucose monitoring equipment and insulin pump/sensor suppliers.
Right Ventricular Longitudinal Strain in Patients with Heart Failure
Authors: Ji Mengmeng , Wu Wenqian , He Lin , Gao Lang , Zhang Yanting , Lin Yixia , Qian Mingzhu , Wang Jing , Zhang Li , Xie Mingxing , Li Yuman
Published: February 2022

Patients with heart failure (HF) have high morbidity and mortality. Accurate assessment of right ventricular (RV) function has important prognostic significance in patients with HF. However, conventional echocardiographic parameters of RV function have limitations in RV assessments due to the complex geometry of right ventricle. In recent years, speckle tracking echocardiography (STE) has been developed as promising imaging technique to accurately evaluate RV function. RV longitudinal strain (RVLS) using STE, as a sensitive index for RV function evaluation, displays the powerfully prognostic value in patients with HF. Therefore, the aim of the present review was to summarize the utility of RVLS in patients with HF.
A Reassessment of the Barrier Effect of the Physis against Metaphyseal Osteosarcoma: A Comprehensive Pathological Study with Its Radiological and Clinical Follow-Up Correlations
Authors: Idoate Miguel Á. , Aquerreta Jesús Dámaso , Lamo-Espinosa José María , San-Julian Mikel
Published: February 2022

Osteosarcoma is a primary malignant bone tumor usually arising at the metaphysis of long bones, particularly around the knee. The physis has been regarded as a barrier capable of blocking tumor extension, thus allowing it to preserve their epiphysis and therefore improve functional results. With the objective of clarifying how effective the physis is as a barrier to tumor spread, a large series of skeletally immature patients with osteosarcoma were reviewed. From 452 metaphyseal osteosarcomas a selection of 282 cases in which the tumor was close or crossing the physis were carried out. This sub-sample was split into two groups according to the surgical treatment (epiphyseal preservation or not). The specimens obtained by resection were studied, and the physeal and metaphyseal areas were studied by multiple sections. Immunostaining against VEGF of physis was obtained in selected cases. In about half of the patients affected by metaphyseal malignant bone tumors, the growth plate and epiphysis were not compromised by the tumor. Three sequential invasive growth patterns of an osteosarcoma in its relationship with the physis could be distinguished. An intense angiogenesis and osteoclastic reaction could be observed in the growth plate in the free zone between the tumor and the physis. The local recurrence incidence was lower in the epiphyseal preservation treated patients than it was in the conventional treatment (8% vs. 12%). Most local recurrences appeared in the first 2 years. The overall survival of patients treated with epiphyseal preservation was better than that of the patients treated without preserving the epiphysis (73% vs. 59%; p = 0.03) at a mean follow-up of 18 years. We have described an angiogenic and osteoclastic reaction in the base of the growth plate in the proximity of the advance front of the tumor, which could facilitate the osteosarcoma invasion. It is also shown that the preoperative imaging method for examination is a valid approach for the decision to carry out epiphyseal preservation. Finally, we concluded that epiphyseal preservation combined with protective chemotherapy is an excellent clinical approach for selected patients with metaphyseal osteosarcoma.
Diagnostic Value of Interferon-Gamma Release Assays for Tuberculosis in the Immunocompromised Population
Authors: Yang Ying , Wang Hong-Jiao , Hu Wei-Lin , Bai Guan-Nan , Hua Chun-Zhen
Published: February 2022

Interferon-gamma release assays (IGRAs) are widely used in the diagnosis of Mycobacterium tuberculosis (M. tuberculosis) infection by detecting interferon-γ released by previously sensitized T-cells in-vitro. Currently, there are two assays based on either enzyme-linked immunosorbent assay (ELISA) or enzyme-linked immunospot (ELISPOT) technology, with several generations of products available. The diagnostic value of IGRAs in the immunocompromised population is significantly different from that in the immunocompetent population because their results are strongly affected by the host immune function. Both physiological and pathological factors can lead to an immunocompromised situation. We summarized the diagnostic value and clinical recommendations of IGRAs for different immunocompromised populations, including peoplewith physiological factors (pregnant and puerperal women, children, and older people), as well as people with pathological factors (solid organ transplantation recipients, combination with human immunodeficiency virus infection, diabetes mellitus, end-stage renal disease, end-stage liver disease, and chronic immune-mediated inflammatory diseases). Though the performance of IGRAs is not perfect and often requires a combination with other diagnostic strategies, it still has some value in the immunocompromised population. Hopefully, the newly developed IGRAs could better target this population.
Correction: Hauck et al. Molecular Orbital Theory—Teaching a Difficult Chemistry Topic Using a CSCL Approach in a First-Year University Course. Educ. Sci. 2021, 11, 485
Authors: Hauck David Johannes , Melle Insa , Steffen Andreas
Published: February 2022

Andreas Steffen was not included as an author in the original publication [...]
Using Group Predictive Voltage and Frequency Regulators of Distributed Generation Plants in Cyber-Physical Power Supply Systems
Authors: Bulatov Yuri , Kryukov Andrey , Suslov Konstantin
Published: February 2022

The widespread use of distributed generation (DG) plants in cyber-physical power supply systems (CPPSS) requires solving the complex problem of setting their regulators. The presented study aimed to determine the performance of the group predictive voltage and frequency regulators of DG plants in CPPSS. These studies were conducted in the MatLab environment on the CPPSS models with gas turbine units and with a small-scale hydroelectric power plant. The proposed method for tuning group predictive regulators makes it possible to improve the quality control indices. The research has established that with an additional load connected, the maximum voltage dip is reduced by a factor of 3.5 compared to conventional control regulators. In addition, the time of a transient process for the generator rotor speed is decreased by a factor of 3. In the case of a short-term short circuit, predictive regulators can reduce the time of the transient process by a factor of 1.5 and the overshoot by more than 2 times. The simulation results have confirmed the efficiency of group predictive regulators when used in DG plants, i.e., improvement of the quality of control processes in various operating modes.
Power Supply of Wireless Sensors Based on Energy Conversion of Separated Gas Flows by Thermoelectrochemical Cells
Authors: Artyukhov Denis , Gorshkov Nikolay , Vikulova Maria , Kiselev Nikolay , Zemtsov Artem , Artyukhov Ivan
Published: February 2022

This article deals with the creation of a power supply system of wireless sensors which take measurements and transmit data at time intervals, the duration of which is considerably less than the activation period of sensors. The specific feature of the power supply system is the combined use of devices based on various physical phenomena. Electrical energy is generated by thermoelectrochemical cells. The temperature gradient on the sides of these cells is created by a vortex tube. A special boost DC/DC converter provides an increase in the output voltage of thermoelectrochemical cells up to the voltage that is necessary to power electronic devices. A supercapacitor is used to store energy in the time intervals between sensor activation. A study of an experimental sample of the power supply system for wireless sensors was conducted. Using the model in MATLAB + Simulink program, the possibility and conditions for creating the considered system for a particular type of wireless sensor were shown.
The Influence of New and Old Energy Labels on Consumer Judgements and Decisions about Household Appliances
Authors: Stasiuk Katarzyna , Maison Dominika
Published: February 2022

Energy efficiency is an increasingly important dimension of household appliances, which is why they are labeled to indicate their energy consumption. In 2020, the European Union countries changed the labeling system from the previous system: ranging from A+++ to D, to the new system: ranging from A to G, assuming it would be more transparent for the consumer. The aim of the study was to find out the extent to which consumers are aware of the new labeling system, and the impact that the new labels have (compared to the previous ones) on the perception of household appliances and consumer decision-making. For this purpose, the survey was conducted on a nationwide representative Polish sample (n = 1054). The research was partly experimental, where the same appliances were presented with new and previous energy labels. The results showed that currently most people do not identify the new energy classes. Furthermore, products with the new labels are perceived as being less energy efficient in comparison with products with the previous labels, which shows that there is some confusion among consumers in terms of the new energy efficiency labeling system.
Efficiency Enhancement of Tilted Bifacial Photovoltaic Modules with Horizontal Single-Axis Tracker—The Bifacial Companion Method
Authors: Wang Shitao , Shen Yi , Zhou Junbing , Li Caixia , Ma Lijun
Published: February 2022

Bifacial photovoltaic modules combined with horizontal single-axis tracker are widely used to achieve the lowest levelized cost of energy (LCOE). In this study, to further increase the power production of photovoltaic systems, the bifacial companion method is proposed for light supplementation and the efficiency enhancement of tilted bifacial modules with a horizontal single-axis tracker. Specifically, a solar reflector is added to the rear end of the tilted bifacial photovoltaic module to guide the sunlight and promote power generation on the rear end. The technical feasibility of the proposed method is verified through optimal calculation and prototype experimental test. The theoretical calculation results suggest that the bifacial companion system is particularly suitable for mid-to-high latitude areas. The higher the latitude, the higher the gain ratio of generated power in the system; there is an optimal module tilt angle that maximizes the efficiency at different latitudes. The closer the module tilt angle to the optimal tilt angle, the higher the power generation efficiency of the system. Meanwhile, compared to the fixed solar reflector, the use of tracking solar reflector is more conducive to improving the power generation efficiency of the system. For the 37.5° latitude area, the annual average power generation gain ratio of the bifacial companion system with tracking solar reflector and fixed solar reflector can reach up to 30% and 17%, respectively. Additionally, the test results for the three sets of bifacial companion prototypes (module tilt angles of 10°, 20°, and 30°) with a fixed solar reflector show that the maximum gain ratio of daily power generation in August 2021 are 8.2%, 13%, and 18.1%, and that in September 2021 are 7%, 8.7% and 13.7%, respectively, which are consistent with the theoretical results.
Effectiveness of Electricity-Saving Communication Campaigns: Neurophysiological Approach
Authors: Borawska Anna , Borawski Mariusz , Łatuszyńska Małgorzata
Published: February 2022

Public communication campaigns are among the tools for promoting electricity saving. A crucial task in the process of creating a campaign is to design a simple message to effectively reach the average consumer. It is a beneficial practice to create alternative messages and pretest them to find the most effective. The research methodology during pretesting includes both quantitative and qualitative methods. However, it is believed that the outcomes obtained with the use of conventional techniques are not fully reliable. Therefore, the following question arises: What additional research methods should be applied at the stage of testing the message of a communication campaign so that its effectiveness can be assessed more reliably and/or improved even before its broadcast? In this study, we aim to present the possibility of applying cognitive neuroscience methods in conjunction with a questionnaire to experimentally check the effectiveness of the message using the example of selected electricity-saving communication campaigns. The key results of this study indicate that merging conscious and subconscious reactions to media messages allows us to gain new knowledge that can be used in the future to improve the communication campaign effectiveness. Our investigation showed the benefits that can be obtained by synergizing traditional research methods with neuroscientific approaches.
Energy Production from Biodegradable Waste as an Example of the Circular Economy
Authors: Szyba Marta , Mikulik Jerzy
Published: February 2022

A growing population, technological progress and economic development result in a constant increase in energy demand. Energy is mostly obtained from fossil energy resources such as coal, natural gas, and crude oil. Burning them leads to air pollution with greenhouse gases (CO2, CH4, NH3 and N2O) and dust (PM2.5 and PM10). They are recognized as the cause of global warming and air pollution. Wind, water, solar and biomass energy are used to eliminate harmful emissions. The latter may come from special plant crops or from biodegradable waste from farming, animal husbandry, the agrifood industry and households. These wastes are transformed into biogas in biogas plants, the basic ingredient of which is methane. Most often, biogas is burned in a cogeneration process, providing electricity and heat. After purification of admixtures, it can be injected into the high-methane gas network or converted into hydrogen in the steam reforming process. In this way, environmentally harmful waste becomes a raw material for energy production, which is an example of a circular economy. The article discusses the functioning of biogas plants in selected EU countries. The current biogas production in Poland was assessed and compared with the production potential of dairy farms. The aim of this article was to show that the production of biogas reduces the emission of greenhouse gases into the atmosphere and the electricity produced from it is not burdened with the cost of purchasing CO2 emission allowances applicable in the EU.
Reinforced Concrete Infilled Frames
Authors: Bagnoli Matteo , Grande Ernesto , Milani Gabriele
Published: February 2022

Masonry-Infilled Reinforced Concrete Frames are a very widespread structural typology all over the world for civil, strategic or productive uses. The damages due to these masonry panels can be life threatening to humans and can severely impact economic losses, as shown during past earthquakes. In fact, during a seismic event, most victims are caused by the collapse of buildings or due to nonstructural elements. The damage caused by an earthquake on nonstructural elements, i.e., those not belonging to the actual structural body of the building, is important for the purposes of a more general description of the effects and, of course, for economic estimates. In fact, after an earthquake, albeit of a low entity, it is very frequent to find even widespread damages of nonstructural elements causing major inconveniences even if the primary structure has reported minor damages. In recent years, many territories have been hit worldwide by strong seismic sequences, which caused widespread damages to the nonstructural elements and in particular to the masonry internal partitions and the masonry infill panels of the buildings in reinforced concrete, with damage to the floor and out-of-plane expulsions/collapses of single layers. Unfortunately, these critical issues have arisen not only in historic, but also in recent buildings with reinforced concrete, in many cases exhibiting inadequate seismic behavior, only partly attributable to the intrinsic vulnerability of the masonry panels against seismic actions. Such problems are due to the following aspects: lack of attention to construction details in the realization of the construction, use of poor-quality materials, and above all lack of design tools for the infill masonry walls. In 2018, regarding the design of nonstructural elements, the formulation of floor spectra has been recently introduced in Italy. This entry article wants to focus on all these aspects, describing the state of the art, the literature studies and the design problems to be solved.
Forest Exploitation and Wood Supply: A Dendroarchaeological Approach between the Massif Central and the Southern Alps since the Middle Ages
Authors: Shindo Lisa , Blondel François , Labbas Vincent
Published: February 2022

Numerous dendroarchaeological studies have been carried out in buildings in the south-eastern quarter of France, which has enabled us to lay the foundations for a first regional restitution of forest stands in the medieval and modern periods, based on the species, diameters, ages and growth rhythms of the trees used by humans. The 2369 pieces of dated softwood timber are mainly larch and fir from the Massif Central and the Alps mountains. Larch seems to have been used mostly locally in the Alps, whereas fir was certainly exported from the two regions to the lowland towns. Very little felling has been identified in the historically troubled 13th–14th century. For the moment we have not identified any fir trees used before the 15th century in the Alps, whereas they are present in the Massif Central from the 12th century. Growth of fir timbers show little variation over time while larch timbers present an increase in growth between those felled until the 12th century and those felled from the 15th century onwards. Finally, since firs from the Massif Central show a higher age trend than those from the Alps, this can serve as a model for identifying the source forests of the timber used in the Rhône valley.
Old-Growth Coniferous Stands on Fertile Drained Organic Soil: First Results of Tree Biomass and Deadwood Carbon Stocks in Hemiboreal Latvia
Authors: Ķēniņa Laura , Zute Daiga , Jaunslaviete Ieva , Samariks Valters , Jansons Āris
Published: February 2022

Organic soils store a large amount of carbon stock, but they are also a large source of greenhouse gas emissions in a forest. Results of previous studies do not provide whole-country representative data of carbon stock in drained fertile organic soil forests in Europe, as the effects of stand age and dominant tree species are significant. Moreover, the growing role of old-growth stands has triggered interest in empirical data about drained organic soils. These data might serve as a reference of theoretical carbon carrying capacity that could be achieved in hemiboreal Latvia. We aimed to characterize tree biomass and deadwood carbon pools in coniferous old-growth stands on fertile, drained organic soils. Seven old-growth Scots pine (Pinus sylvestris) and Norway spruce (Picea abies) dominated stands (131–174 years) were measured. Both groups of stands had similar carbon stocks, reaching 167 and 154 t C ha−1 in tree biomass and 11 and 10 t C ha−1 in deadwood, respectively. A large variation in deadwood carbon storage was found across sample plots, ranging from 0.6 to 26.6 t C ha−1. Dead standing trees and downed logs store a great share of the total deadwood carbon, 5 and 4 t C ha−1, respectively. Significantly less carbon was stored in dead standing trees with broken tops (1 t C ha−1). Further assessment of soil carbon stock and fluxes is ongoing to reduce uncertainty in the soil carbon evaluation of old-growth stands in the context of climate change mitigation targets in a hemiboreal region.
Reducing Slipstream Velocities Experienced in Proximity to High-Speed Trains
Authors: Dunlop Jordan Ashley , Thompson Mark Christopher
Published: February 2022

Slipstream wake structures generated by the passing of high-speed rail vehicles represent a hazard to passengers and workers in close proximity. In this article, the possibility of reducing peak slipstream velocities through the implementation of angled fins or swirling flow injection is assessed on the basis of improved delayed detached eddy simulations (IDDES). The key to improving slipstream velocities involves redirecting and/or reducing the internal energy, a pair of meandering counter-rotating vortex cores that are associated with large wake slipstream deviations. It is demonstrated that the danger imposed by slipstream wake structures, as measured by the induced velocity measures recorded at a series of test points adjacent to the passing train, could be significantly reduced, with decreases from 10% up to 23%, recorded across a range of sampling locations. The means by which these reductions are generated and the corresponding changes in the flow are also explored through analysis of the modified wakes. As such, these devices show promise at improving the operational safety of high-speed rail vehicles.
Food Safety Assessment of Commercial Genetically Modified Soybeans in Rats
Authors: Lin Huan-Yu , Liao Jiunn-Wang , Chen Ruo-Shiuan , Chang Chia-Hua , Chang Hui-Wen , Chang Shih-Chieh , Chu Wen-Shen , Lin Chien-Ku , Lin Hsin-Tang
Published: February 2022

Although the safety of commercial genetically modified (GM) soybeans has been well evaluated and GM soybeans are legally sold under government management, some consumers still have concerns about their safety. The objective of this study was to evaluate the safety of commercial GM soybeans sold in markets as a food source. In the present study, two commercial GM (GM-1 and -2) soybeans and one non-GM soybean were randomly purchased and subjected to a whole food toxicity assessment. Rats (SD), male and female, were divided into six groups (10/sex/group). Two dosages of 1 g/kg/day and 5 g/kg/day of soybeans were selected for the low- and high-dose groups. Rats were administered the soybeans via daily oral fed for 90 days. The results indicate that the body weight, organ weight, biochemistry, hematology, and urology showed no biologically adverse effects. At necropsy, no significant differences between organ weights were noted between the non-GM- and GM soybeans-treated groups. Moreover, no gross or histopathological lesions were observed in the high-dosage (5 g/kg/day) fed groups of the non-GM and GM soybean fed rats. In conclusion, this food safety assessment revealed that commercial GM soybeans are substantially equivalent to non-GM soybeans in rats.
Effect of Anti-Obesity and Antioxidant Activity through the Additional Consumption of Peel from ‘Fuji’ Pre-Washed Apple
Authors: Ko Da-Yeong , Ku Kang-Mo
Published: February 2022

There is limited information on the health effects of apple peel taken from ‘Fuji’ (Malus pumila Mill) apples washed with ozonated water. To clarify the health-promoting effects of peel, the triterpenoids (ursolic acid and oleanolic acid) were quantified with gas chromatograph–mass spectrometry. Anti-obesity effects of apple peel extract on the 3T3-L1 pre-adipocyte cell were compared with apple flesh, whole apple, and ursolic acid. The peel extract treatment with 3.30 ± 1.05 μM of ursolic acid significantly suppressed (p < 0.05) the lipid accumulation compared with the content in flesh, and a similar level was reached in the 5 μM ursolic acid positive control group. In the peel extract and ursolic acid treatment groups, the C16:0 concentration was significantly inhibited (p < 0.05), implying the anti-obesity effect of ursolic acid on the 3T3-L1 cell. Moreover, apple peel contributed 41% of the total flavonoids content and 31% of the phenolic contents of the whole apple, but only accounted for less than 10% of the whole apple (weight basis). This study’s results offer basic data on pre-washed apple as a health functional food, offering information about the health benefits of apple peel, calculated based on the partial ratio in the whole apple.
A National French Consensus on Gene List for the Diagnosis of Charcot–Marie–Tooth Disease and Related Disorders Using Next-Generation Sequencing
Authors: Benquey Thibaut , Pion Emmanuelle , Cossée Mireille , Krahn Martin , Stojkovic Tanya , Perrin Aurélien , Cerino Mathieu , Molon Annamaria , Lia Anne-Sophie , Magdelaine Corinne , Francou Bruno , Guiochon-Mantel Anne , Malinge Marie-Claire , Leguern Eric , Lévy Nicolas , Attarian Shahram , Latour Philippe , Bonello-Palot Nathalie
Published: February 2022

Next generation sequencing (NGS) is strategically used for genetic diagnosis in patients with Charcot–Marie–Tooth disease (CMT) and related disorders called non-syndromic inherited peripheral neuropathies (NSIPN) in this paper. With over 100 different CMT-associated genes involved and ongoing discoveries, an important interlaboratory diversity of gene panels exists at national and international levels. Here, we present the work of the French National Network for Rare Neuromuscular Diseases (FILNEMUS) genetic diagnosis section which coordinates the seven French diagnosis laboratories using NGS for peripheral neuropathies. This work aimed to establish a unique, simple and accurate gene classification based on literature evidence. In NSIPN, three subgroups were usually distinguished: (1) HMSN, Hereditary Motor Sensory Neuropathy, (2) dHMN, distal Hereditary Motor Neuropathy, and (3) HSAN, Hereditary Sensory Autonomic Neuropathy. First, we reported ClinGen evaluation, and second, for the genes not evaluated yet by ClinGen, we classified them as “definitive” if reported in at least two clinical publications and associated with one report of functional evidence, or “limited” otherwise. In total, we report a unique consensus gene list for NSIPN including the three subgroups with 93 genes definitive and 34 limited, which is a good rate for our gene’s panel for molecular diagnostic use.
Actionable Genomics in Clinical Practice: Paradigmatic Case Reports of Clinical and Therapeutic Strategies Based upon Genetic Testing
Authors: Butler Merlin G. , Moreno-De-Luca Daniel , Persico Antonio M.
Published: February 2022

In clinical settings, the information provided by genetic testing can explain the triggers and processes underlying clinical presentations, such as neurodevelopmental disorders, in up to one third of affected individuals. However, translating this knowledge into better and more personalized clinical management to many appears a distant target. This article presents three paradigmatic cases to exemplify how this translational effort can, at least in some instances, be undertaken today with very positive results: (a) a young girl carrying a chr. 16p11.2 duplication can be screened using targeted exams and undertake therapeutic/preventive interventions related to her genetic diagnosis; (b) a 13-year-old boy with intellectual disability and autism spectrum disorder carries a chr. 11q14.1 deletion, partly spanning the DLG2 gene important for synaptic function, and gained over 20 I.Q. points ostensibly due to carbolithium, prescribed in the absence of affective symptoms, exclusively following the pathophysiology pointed out by the genetic results; (c) a 58-year-old woman carries a COL3A1 gene variant responsible for the vascular form of Ehler–Danlos syndrome with colon rupture. Detection of this variant in six members of her extended family allows for better clinical management of the proband and targeted genetic counselling for family members at risk of this connective tissue disorder. The unprecedented flow of genetic information available today through new technologies, if interpreted in the light of current knowledge in clinical diagnosis and care of those with connective tissue disorders and neurodevelopmental disturbances, in biology and in neuropsychopharmacology, can promote better clinical and pharmacological treatment, disease surveillance, and management provided and incorporated into the clinical setting.
Effect of E-64 Supplementation during In Vitro Maturation on the Developmental Competence of Bovine OPU-Derived Oocytes
Authors: Balboula Ahmed Z. , Aboelenain Mansour , Sakatani Miki , Yamanaka Ken-Ichi , Bai Hanako , Shirozu Takahiro , Kawahara Manabu , Hegab Abd Elraouf O. , Zaabel Samy M. , Takahashi Masashi
Published: February 2022

Recovery of bovine oocytes using the ovum pick-up (OPU) technique offers the advantage of rapid genetic improvement through propagation of desired genes from animals with high genetic qualities. However, the developmental competence of OPU-derived immature oocytes remains relatively poor. We previously found that cathepsin B gene expression and activity are increased in poor quality oocytes and embryos compared to good quality ones. In this study, we investigated the effect of E-64 (cathepsin B inhibitor) supplementation during in vitro maturation (IVM) on the developmental competence of OPU-derived immature oocytes and the quality of the produced blastocysts. Our results showed that supplementation of IVM medium with E-64 significantly improved the developmental competence of OPU-derived immature oocytes as evidenced by the significant increase of the blastocyst rate. Importantly, the presence of E-64 during IVM also significantly improved blastocyst quality by increasing the total cell number and decreasing the percentage of TUNEL positive cells. These results indicate that E-64 supplementation during IVM is a promising tool to improve the efficiency of OPU-IVF program by improving the developmental competence of OPU-derived immature oocytes.
Regulating Vapor Pressure Deficit and Soil Moisture Improves Tomato and Cucumber Plant Growth and Water Productivity in the Greenhouse
Authors: Song Xiaoming , Miao Lili , Jiao Xiaocong , Ibrahim Musawar , Li Jianming
Published: February 2022

Atmospheric vapor pressure deficit (VPD) is the driving force that regulates the rate of water transport within plants. Under High VPD (HVPD), plants always reduce their photosynthesis rate and close their stomata. Experiments were performed under greenhouse conditions with cucumber and tomato plants to identify the regulatory effect of VPD on plant water capacity. Treatments included two levels of soil water (100% and 60% field capacity [FC]) combined with two levels of VPD (LVPD and HVPD). Results indicated that with 60%FC, the plant heights of tomato and cucumber were enhanced under LVPD compared with those under HVPD. With 60%FC, relative leaf water contents under LVPD increased by 11% compared with those under HVPD. Furthermore, LVPD significantly improved the photosynthetic capacity of the two crops and changed their stress responses. Our results indicated that LVPD at different soil moisture levels reduced irrigation demand under greenhouse conditions. This approach can be applied in water management in greenhouse vegetable production in China and other regions of the world with temperate continental climates.
The Spirit of Adventure: A Driver of Attractiveness of the Hospitality Industry for Young People during a Pandemic Crisis
Authors: Burlea-Schiopoiu Adriana , Baldo Mara Del , Idowu Samuel O.
Published: February 2022

The COVID-19 pandemic has strongly affected tourism and leisure activities worldwide, especially in the hospitality and tourism sectors. Within this context, this study aimed to evaluate the impact of the pandemic on the future attractiveness of the hospitality industry (HI) to young people. The conceptual model underpinning the empirical research proposes a direct relationship between job attractiveness and the spirit of youth adventure. Findings prove that young people are enthusiastic about working in the HI because they can easily practice their creative ideas. Communicating with other people and dealing with clients’ complaints is challenging for them in the pandemic crisis created by COVID-19. The results are of interest to policymakers in terms of suggestions on how to transform the challenges into opportunities by using the constraints imposed by the pandemic crisis that have limited the socialisation between people, enhancing the creativity of young people, and motivating them to work in the HI. Moreover, our research provides managers and other decisionmakers with some motivational factors to increase the attractiveness of their companies to young people and suggestions helpful to scholars involved in HI research to cultivate resilience capabilities by giving them inherent skills.
Diet Quality and Dietary Inflammatory Index Score among Women’s Cancer Survivors
Authors: Kranz Sibylle , Hasan Faten , Kennedy Erin , Zoellner Jamie , Guertin Kristin A , Shivappa Nitin , Hébert James R , Anderson Roger , Cohn Wendy
Published: February 2022

The purpose of this study was to investigate Healthy Eating Index 2015 (HEI-2015) and Energy-Adjusted Dietary Inflammatory Index (E-DIITM) scores in women’s cancer survivors and to examine socio-economic (SES) characteristics associated with these two diet indices. In this cross-sectional study, survivors of women’s cancers completed a demographic questionnaire and up to three 24-h dietary recalls. HEI-2015 and E-DII scores were calculated from average intakes. One-way ANOVA was used to examine the association of various demographic factors on HEI-2015 and E-DII scores. Pearson Correlation was used to calculate the correlation between the two scores. The average HEI-2015 score was 55.0 ± 13.5, lower than the national average, and average E-DII was −1.14 ± 2.24, with 29% of women having a more pro-inflammatory and 71% a more anti-inflammatory diet. Diets with higher HEI-2015 scores were associated with more anti-inflammatory diets (r = −0.67, p < 0.001). Those having a graduate degree (F(2,49) = 3.6, p = 0.03) and completing cancer treatment > 4 years ago (F(2,49) = 4.8, p = 0.01) had higher HEI-2015 scores. There were no associations between SES and E-DII scores. The diet quality of women’s cancer survivors is comparatively low, but many achieved an anti-inflammatory diet; a promising avenue for preventing recurrence. There is an urgent need to involve health care professionals in the guidance of women’s cancer survivors to improve diet quality and prevent cancer recurrence.
Reducing Obesogenic Eating Behaviors in Hispanic Children through a Family-Based, Culturally-Tailored RCT: Abriendo Caminos
Authors: Barragan Maribel , Luna Viridiana , Hammons Amber J. , Olvera Norma , Greder Kimberly , Drumond Andrade Flavia Cristina , Fiese Barbara , Wiley Angela , Teran-Garcia Margarita ,   the Abriendo Caminos Research Team
Published: February 2022

Family-based interventions that incorporate culturally-tailored multi-component curricula and are grounded on evidence-based information and theoretical frameworks can help reduce the prevalence of obesity among Hispanic children. Abriendo Caminos: Clearing the Path to Hispanic Health is a multi-site culturally-tailored randomized control trial that aims to reduce obesity rates in Hispanic families by delivering education on nutrition, family wellness, and physical activity. This study evaluated the effect of the Abriendo Caminos six-week intervention on dietary behaviors of Hispanic children (6–18 years). Mothers (n = 365) reported their child’s eating behavior intake using the U.S. Department of Education’s Early Childhood Longitudinal Study protocol (ECLS). Pre/post dietary changes were evaluated using separate generalized estimating equation models adjusted for site, child sex, and child age group. Findings indicate a reduction in the frequency of sugar-sweetened beverages (OR 0.55, 95% CI 0.35, 0.87, p = 0.01), French fries (OR 0.56, 95% CI 0.36, 0.86, p = 0.009), and fast food (OR 0.55, 95% CI 0.36, 0.84, p = 0.006) consumption among children in the intervention arm. Additionally, children in the intervention arm increased their frequency of vegetable consumption (OR 1.84, 95% CI 1.08, 3.12, p = 0.03). The Abriendo Caminos intervention effectively improved four of eight eating behaviors in a short-term intervention.
User Experience and Sustainability of 3D Printing in Dentistry
Authors: Hegedus Tamas , Kreuter Patrik , Kismarczi-Antalffy Aron Attila , Demeter Tamas , Banyai Dorottya , Vegh Adam , Geczi Zoltan , Hermann Peter , Payer Michael , Zsembery Akos , Al-Hassiny Ahmad , Mukaddam Khaled , Herber Valentin , Jakse Norbert , Vegh Daniel
Published: February 2022

Background: 3D printing is a rapidly developing technology in the healthcare industry and in dentistry. Its application clearly shows that this area of digital dentistry has potential for everyday usage across all fields, including prosthodontics, orthodontics, maxillofacial surgery, and oral implantology. However, despite gaining ground, there is a lack of information about how specialists (dentists and dental technicians) use additive technology. Our research group aimed to investigate the impact of social media on additive manufacturing technology among dental specialists and their everyday usage of 3D printing. Methods: This paper investigated specialists’ everyday usage of 3D printers via an online survey (Google Forms). The survey questions aimed to discover the number of 3D printers used, the accessibility of the devices, the annual cost, and the design programs. Since specialists tend to build online communities on social media, we circulated our study questionnaire using our profiles on LinkedIn, Facebook, and Instagram platforms during our research. Results: A total of 120 responses were received from 20 countries, with the most significant numbers being from Hungary 23.7% (n = 27), the United States 18.4% (n = 21), and the United Kingdom 7.9% (n = 9). Most of the participants were dentists (n = 68) or dental technicians (n = 29), but some CAD/CAM specialists (n = 23) also completed our survey. The participants had an average of 3.8 years (±0.7) of experience in the 3D printing field, and owned a total of 405 printing devices (3.6 on average/person). Conclusions: The impact of social media on this research field is growing increasingly. Hence, we support specialists in joining virtual communities on professional platforms. This article intended to provide a practical overview, feedback, and direction for dentists interested in 3D printing technology. From our survey, we can conclude that additive technology is broadening dental applications and the services that we can provide for our patients.