bluuebunny/crossref_metadata_2025 huggingface dataset:
Kolofon S7
Authors: Christoffersen Erik Exe
Published: May 2024

Peripeti
© Peripeti og forfatterne
 
Temaredaktion
Susanne Andreasen, Knut Ove Arntzen, Annelis Kuhlmann og Birgit Kleist Pedersen
Redaktion
Janicke Branth, Erik Exe Christoffersen, Solveig Daugaard, Michael Eigtved, Solveig Gade, Falk Heinrich, Kjersti Hustvedt, Jens Christian Lauenstein Led, Johan Holm Mortensen, Susanne Hjelm Pedersen, Thomas Rosendal Nielsen, Laura Luise Schultz (ansv.), Mads Thygesen, Mette Tranholm og Cecilie Ullerup Schmidt
Layout: Lho Brockhoff
Omslagsfoto: Danseren Elisabeth Heilmann Blind fra forestillingen Angerlarsimaffeqanngitsoq IHjemløs ( Homeless (2019). Koreografi og dramaturgi: Ada Einmo Jiirgensen. Foto: Petvin Sandstri:im
 
ISSN: 1604-0325
Peripeti udgives af Afdeling for Dramaturgi (Aarhus Universitet) i samarbejde med Teater- og Performancestudier (Københavns Universitet) og Dramatikeruddannelsen (Den Danske Scenekunstskole) Dette nummer udgives i samarbejde med Nunatta Isiginnaartitsisarfia / Tue National Theatre of Greenland / Grønlands Nationalteater, Ilisimatusarfik / Grønlands Universitet/ University of Greenland og Universitetet i Bergen. Der er modtaget støtte fra Augustinus Fonden, Hans Lynge Fonden, Nunatta Isiginnaartitisiarfia / Tue National Theatre of Greenland / Grønlands Nationalteater og Aarhus Universitet Nummeret indeholder såvel essays som fagfællevurderede bidrag
Bestilling og abonnement
Løssalg 100 kr. Internet: http://www.peripeti.dk/bestilling/
Peripeti som digitalt tidsskrift kan findes på hjemmesiden https://tidsskrifr.dk/peripeti
Redaktionsadresse
www.peripeti.dk Peripeti
Afdeling for Dramaturgi
Institut for Kommunikation og Kultur, Aarhus Universitet Langelandsgade 139.
DK 8000 Århus C
 
Redaktion: laura@hum.ku.dk; soga@scenekunstskolen.dk
Kolofon P33
Authors: Rosendal Nielsen Thomas
Published: May 2024

Peripeti 32
© Peripeti og forfatterne
Temaredaktion
Falk Heinrich, Susanne Hjelm Pedersen, Lotte Løvholm, Sidsel Nelund, Laura Luise Schultz, Ralf Richardt Strøbech
Redaktion
Janicke Branth, Josefine Brink Siem, Erik Exe Christoffersen, Solveig Daugaard, Michael Eigtved, Solveig Gade, Falk Heinrich, Susanne Hjelm Pedersen, Johan Holm Mortensen, Storm Møller Madsen, Thomas Rosendal Nielsen, Laura Luise Schultz (ansv.), Mads Thygesen, Cecilie Ullerup Schmidt
Layout:
Ivan Micic
 
ISSN: 1604-0325
Peripeti udgives af Afdeling for Dramaturgi (Aarhus Universitet) i samarbejde med Teater- og Performancestudier (Københavns Universitet) og Den Danske Scenekunstskole. Peripeti er støttet af Statens Kunstfond og Danmarks Frie Forskningsfond.
Redaktionsadresse
www.peripeti.dk
PeripetiAfdeling for DramaturgiInstitut for Kommunikation og Kultur, Aarhus UniversitetLangelandsgade 139.DK 8000 Århus C
Redaktion: laura@hum.ku.dk solveigg@hum.ku.dk aekexe@cc.au.dk
FIX&FOXY’s Distribution of Management
Authors: Krøgholt Ida
Published: May 2024

Krøgholt is unfolding FIX&FOXY’s way of working as distributed facilitation, where the participants facilitate each other, and thus through the theatre process develop an awareness of communication and observation.
Conspiracy hippies and Holocaust denial
Authors: Schultz Laura Luise
Published: May 2024

While the artists collective Center for Political Beauty address contemporary politicians’ open or tacit denial of Holocaust and the political betrayals of the 1930s in their social interventions, the artists collective Frankfurter Hauptschule expose the intricate entanglements of fascism with hippie-inspired esotericism and a new interest in German Romanticism and Nordic mysticism among young German artists today. The article will analyze the activist practice of the two collectives and discuss what role artistic interventions into politics may play in the fight against the fascist aestheticization of the political in Benjamin’s sense.
Kolofon P37
Authors: Rosendal Nielsen Thomas
Published: May 2024

Peripeti 38
© Peripeti og forfatterne
Temaredaktion
Sandra Buch, Solveig Gade, Lise Sofie Houe, Thomas Rosendal Nielsen, and Yvonne Schmidt.
Redaktion
Erik Exe Christoffersen, Solveig Daugaard, Michael Eigtved, Solveig Gade, Storm Møller Madsen, Thomas Rosendal Nielsen (ansv.), Signe Nygaard, Cecilie Ullerup Schmidt, Laura Luise Schultz, Josefine Brink Siem.
Korrektur:
Marianne Ølholm
Omslag:
HABITAT+Bodyscaping 2021
Nana Francisca Schottländer
Produceret af Metropolis
Foto: Marine Gastineau
Layout:
Ivan Micic
ISSN: 1604-0325
Peripeti udgives af Afdeling for Dramaturgi (Aarhus Universitet) i samarbejde med Teater- og Performancestudier (Københavns Universitet) og Den Danske Scenekunstskole. Peripeti er støttet af Statens Kunstfond og Danmarks Frie Forskningsfond.
Redaktionsadresse:
www.peripeti.dk
Peripeti
Afdeling for Dramaturgi
Institut for Kommunikation og Kultur, Aarhus Universitet
Langelandsgade 139.
DK 8000 Århus C
 
Redaktion: thomas.rosendal@cc.au.dk
Den gravide krop som prisme
Authors: Kolmos Marie-Louise
Published: May 2024

Ud fra forfatterens erfaringer som forestillingsleder på forestillingen Frankly my dear I don’t give a damn (Aarhus Teater sæson 2022/23) udforsker denne artikel de æstetisk-affektive aspekter af scenekunstnerisk repræsentation af graviditet og moderskab. Forfatteren bruger den gravide krop som prisme i udviklingen af en analysemodel til æstetisk-affektiv repræsentationsanalyse. Dette gøres med rod i Karen Barads nymaterialistiske teori, agential realisme. Modellen fremhæver publikums rolle i repræsentationsanalysen ved at gøre selve sammenhængen mellem æstetik, affekt og repræsentation til sit genstandsfelt.
Dødens dramaturgiske potentiale
Authors: Garde Bergmann Majse
Published: May 2024

I "Dødens dramaturgiske potentiale" ser Majse Garde nærmere på "døden" som et centralt tema i værker af henholdsvis Astrid Saalbach (Det kolde hjerte, 2002), Line Knutzon (Den luft andre indånder, 2001) og Nikoline Werdelin (Boblerne i bækken, 2002). Med afsæt i sociologien indkredser artiklen hvordan dødssituationen anvendes som et dramatisk set-up, og diskuterer de dramaturgiske implikationer.
[This issue has been reconstructed from a scanned version of a printed issue, which might result in minor flaws in the text. This is also the cause of the odd page breaks.]
Kompleks dramaturgi
Authors: Szatkowski Janek
Published: May 2024

I artiklen "Kompleks dramaturgi - studie i Christian Lollikes dramatik" diskuterer Janek Szatkowski, hvordan dramatikeren gennem en række parabasiske udsigelseskonstruktioner rejser et udsigtspunkt, hvorfra han kan beskrive, hvordanværdier baseres og håndteres i det senmoderne. Den analytiske tilgang består i en kombination af indsigter fra udsigelsesanalysen og systemteori, hvorigennemSzatkowski beskriver den særlige kompleksitetsdramaturgi, man møder hos Lollike.
[The issue has been reconstructed from a scanned version of a printed issue, which might result in minor flaws in the text. This is also the cause of the odd page breaks.]
Eksperimenterende realismeformer
Authors: Christoffersen Erik Exe
Published: May 2024

I artiklen "Eksperimenterende realismeformer", ser Erik Exe Christoffersen nærmere på, hvordan fortællepositionen og opførelsens realitet tematiseres i en række dramaer og teaterforestillinger. Det er karakteristisk for disse værker, at densamtidige tilstedeværelse af performere og tilskuere indtager en central funktion i værkkonstruktionen. Samtidigt fremhæver Christoffersen, hvordan dette forhold ikke er uforeneligt med en genskrivning eller overmaling af traditionen, f.eks. hosAstrid Saalbach og Jens Christian Grøndahl.
[The issue has been reconstructed from a scanned version of a printed issue, which might result in minor flaws in the text. This is also the cause of the odd page breaks].
Teatrets kunststatus - i virkelighedens navn
Authors: Christoffersen Erik Exe
Published: May 2024

I artiklen "Teaterkunstens status og funktion - i virkelighedens navn" diskuterer Erik Exe Christoffersen blandt andet politikeren Louise F reverts forsøg på at gribe ind overfor Teater Katapult (Århus) i forbindelse med Christian Lollikes stykke, Dom over skrig (2004), om gruppevoldtægt. Med afsæt i en analyse af, hvordan teaterkunsten udnytter virkeligheden og skaber nye alliancer, skriver Christoffersen om det konkrete samspil med den kulturelle kontekst, som teksten indgår i. Artiklen kan læses som et forsvar for kunstautonomien og tilskuerens kunstdom. Overfor det politiske systems krav om en statsautoriseret kanon, derskal bære et bestemt nationalt dannelsesideal, hævder Christoffersen, at et sådant krav vil være blokerende for den frie udøvelse af kunstdomme. 
[The issue is reconstructed from a scanned copy. This might cause minor flaws in the text. This is also the cause of the odd page breaks].
At performe eller ikke at performe
Authors: Skjoldager-Nielsen Kim
Published: May 2024

Focusing on the experience of the audience, Kim Skjoldager-Nielsen analyzes two performances by Danish director Signa Sørensen in order to sum up some dramaturgical perspectives of this particular kind of interactive theater.
Performance som verdensskabelse
Authors: Qvortrup Lars
Published: May 2024

Lars Qvortrup andmelder Hotel Pro Formas H. C. Andersen-forestilling: Jeg er kun skindød (2005).
En tradition af opbrud
Authors: Løhmann Stephensen Jan
Published: May 2024

Anmeldelse af En tradition af opbrud. Avantgardernes tradition og politikRedigeret af Tania Ørum, Marianne Ping Huang og Charlotte EngbergForlaget Spring, 2005
Kolofon P7
Authors: Christoffersen Erik Exe
Published: May 2024

Peripeti
© Peripeti og forfatterne
 
TEMAREDAKTION
Erik Exe Christoffersen, Laura Schultz, Solveig Gade og Jens Christian Lauenstein Led
 
REDAKTION
Erik Exe Christoffersen (ansvarshavende), Janicke Branth, Falk Heinrich, Mads Thygesen, Thomas Rosendal Nielsen, Birgitte Hesselaa, Laura Schultz, Solveig Gade, Michael Eigtved, Jens Christian Lauenstein Led og Jeppe Sejer Sørensen (PR).
Layout: Christian Brink Christensen og Mads Thygesen
Omslag: The Democ rac y (Foto: Das Beckwerk)
Tryk: Werks Offset
Oplag: 500
ISSN: 1604-0325
REDAKTIONSADRESSE
Peripeti
Afdeling for Dramaturgi Institut for Æstetiske Fag, Aarhus Universitet, Langelandsgade 139,
DK - 8000 Århus C.
E-mail: aekexe@hum.au.dk
BESTILLING OG ABONNEMENT
peripeti@hum.au.dk Abonnement (to numre): 120 kr. Løssalg 75 kr.
Internet: http://www.dramaturgi.au.dk/forskning/publikationer/peripeti
 
Peripeti udgives af Afdeling for Dramaturgi (Aarhus Universitet) i samarbejde med Teatervidenskab (Københavns Universitet) og Dramatikeruddannelsen (Aarhus Teater) Tidsskriftet udgives med støtte fra Forskningsrådet for Kultur og Kommunikation. Alle temaartikler gennemgår et peer review.
 
Peripeti udkommer to gange om året.
 
Planlagte udgivelser: Teatralitet (forår 2007), Teatertekster (efterår 2007).
 
Tidligere udgivelser: 1) Jon Fosse, 2) Why a Theatre Laboratory?, 3) Ny dansk dramatik,
4) Dogme og dramaturgi, 5) Henrik Ibsen.
Guaianolide–type sesquiterpene lactones from the aqueous extract of Salcedoa mirabaliarum
Authors: Castillo Quírico A. , Vicente Pierina E. , Díaz Luis A. , Sprules Tara , Matos-Espinosa Carime , Vólquez Yanet
Published: May 2024

The phytochemical study of the aqueous extract of Salcedoa mirabaliarum (aerial parts), afforded five guaianolide– type sesquiterpene lactones: dihydroestafiatone, zaluzanin C, dihydroestafiatol, isoamberboin, and 4–epi– dihydroestafiatol, detected for first time in this species. The chemical structures were identified using 13C Nuclear magnetic resonance by comparison with previously reported data.
Postoperative Opioid-Prescribing Practice in Limb Preservation Surgery
Authors: Brooks Brandon M. , Shih Chia-Ding , Bratches Reed W. R. , Pham Kevin T. , Brooks Bradley M. , Hooshivar Lili , Wolff Kristina B.
Published: May 2024

Background: Limb preservation surgery affects more than 100,000 Americans annually. Current postoperative pain management prescribing practices of podiatric physicians in the United States are understudied. We examined prescribing practices for limb preservation surgery to identify prescriber characteristics' that may be associated with postoperative opioid-prescribing practices.

Methods: We administered an anonymous online questionnaire consisting of five patient scenarios with limb preservation surgery commonly performed by podiatric physicians. Respondents provided information about their prescription choice for each surgery. Basic provider demographics were collected. We developed linear regression models to identify the strength and direction of association between prescriber characteristics and quantity of postoperative opioid “pills” (dosage units) prescribed at surgery. Logistic regression models were used to identify the odds of prescribing opioids for each scenario.

Results: One hundred fifteen podiatric physicians completed the survey. Podiatric physicians reported using regional nerve blocks 70% to 88% of the time and prescribing opioids 43% to 67% of the time across all scenarios. Opioids were more commonly prescribed than nonsteroidal anti-inflammatory drugs and anticonvulsants. Practicing in the Northeast United States was a significant variable in linear regression (P = .009, a decrease of 9–10 dosage units) and logistic regression (odds ratio, 0.23; 95% confidence interval, 0.07–0.68; P = .008) models for the transmetatarsal amputation scenario.

Conclusions: Prescribing practice variation exists in limb preservation surgery by region. Podiatric physicians reported using preoperative regional nerve blocks more than prescribing postoperative opioids for limb preservation surgeries. Through excess opioid prescribing, the diabetes pandemic has likely contributed to the US opioid epidemic. Podiatric physicians stand at the intersection of these two public health crises and are equipped to reduce their impact via preventive foot care and prescribing nonopioid analgesics when warranted.
Balance performance, falls-efficacy and social participation in patients with type 2 diabetes mellitus with and without vestibular dysfunction
Authors: Komalasari Dwi Rosella , Vongsirinavarat Mantana , Hiengkaew Vimonwan , Nualnim Nantinee
Published: May 2024

Background

The performance of balance is an important factor to perform activities. The complications of type 2 diabetes mellitus (T2DM), especially vestibular dysfunction (VD), could decrease balance performance and falls-efficacy (FE) which consequently impacts social participation and quality of life (QoL).

Purpose

This study aimed to compare balance performance, FE, social participation and QoL between individuals with T2DM with and without VD.

Methods

The participants comprised 161 T2DM with VD and 161 without VD. Three clinical tests used for confirming VD included the Head Impulse Test (HIT), the Dix Hallpike Test (DHT) and the Supine Roll Test (SRT). The scores of static and dynamic balances, FE, social participation and QoL were compared between groups.

Results

The balance performance, FE, social participation and QoL were lower in the group with VD. The number of patients who had severe social restriction was higher in T2DM with VD than without VD (58.4% vs 48.4%). Moreover, all domains of QoL (physical, psychological, social relationships and environmental) were lower in T2DM with VD than without VD.

Conclusion

The presence of VD in T2DM patients was associated with decreased physical balance performances and increased social and QoL disengagement. Comprehensive management related to balance and FE, as well as the monitoring to support social participation and QoL, should be emphasized in patients with T2DM with VD.
Análisis sociopragmático de un poco en las entrevistas semidirigidas
Authors: Repede Doina
Published: May 2024

El objetivo de este trabajo consiste en realizar un análisis cualitativo y cuantitativo de los distintos usos y funciones de la locución un poco, así como su distribución sociopragmática, entre hablantes de español de la comunidad urbana de Sevilla. En concreto, se trata de una muestra constituida de 48 entrevistas semidirigidas correspondientes al sociolecto alto y bajo, recogidas entre 2009 y 2018 y que forman parte del corpus PRESEEA en la ciudad hispalense (Repede, 2019a, 2019b). Los resultados muestran que la estructura un poco se utiliza en la ciudad hispalense ampliamente como operador discursivo con valor atenuador, aproximador, intensificador y reformulador. Asimismo, la función más empleada es la atenuadora, que sirve generalmente para proteger y salvaguardar la imagen del hablante. Además, el uso de un poco mitigador está más extendido entre las mujeres, mientras que la función aproximadora aparece más en los hombres.
Consumer Satisfaction with Food Grains Distributed through Public Distribution Systems in Kerala and Andhra Pradesh- An Economic Analysis
Authors: D Vasipally , T P Lazarus, , Thasnimol F , A C Chandran, , P P Gopinath,
Published: May 2024

The study analyzed consumer satisfaction with food grains distributed through the public distribution system in Kerala and Andhra Pradesh. A multi-stage random sampling technique was adopted for the data collection and information was obtained from 240 respondents. Percentages and Likert’s Scale Technique were used to analyze the level of consumer satisfaction. The survey in Kerala and Andhra Pradesh gauges public perceptions and satisfaction with the Public Distribution System (PDS). Key findings revealed that a notable satisfaction rate in both states regarding the quality of food grains, the helpfulness of PDS dealers, and the functioning of local outlets. While uncertainties persist regarding accessibility, regularity, and quantity sufficiency, the majority of the respondents in both states opined that PDS is significantly contributing to household food security. Respondents express contentment with billing systems, with slightly higher satisfaction in Kerala, and commend the E-POS and biometric systems. Security measures, such as biometric authentication, receive positive feedback. Overall, the survey indicates positive sentiments towards the PDS in both states, highlighting its pivotal role in ensuring food security and meeting public expectations.
The Influence of Implementing Digital Marketing and Experiential Marketing on Brand Loyalty Mediated by Brand Trust
Authors: Hasan Israk Fabian , Astuti Budi , Hidayat Anas
Published: May 2024

This research aims to examine the effect of implementing digital marketing and experiential marketing on brand loyalty through brand trust in coffee shops in the city of Yogyakarta, Indonesia. In this research, the population used was coffee shop customers in the city of Yogyakarta, Indonesia. The number of samples taken was 266 respondents. This research data was obtained using an online survey questionnaire via Google Form with a measurement scale using a 5-point Likert scale. This research is descriptive research using quantitative methods. The analytical tool used is Structural Equation Modeling (SEM), with data analysis techniques using the AMOS application version 23.0 and Sobel Test. The results of the analysis show that the influence of digital marketing implementation has a significant effect on brand loyalty, brand trust has a significant effect on brand loyalty and experiential marketing has no significant effect on brand trust and brand loyalty and digital marketing have no significant effect on brand trust. The results of this research can help the coffee shop industry, especially in the Indonesian context, in understanding consumer behavior and the factors that contribute to customer trust in a brand.
Efficiency of Four Reproductive Hormones in the Artificial Propagation of Heterotis niloticus (Cuvier, 1829)
Authors: Asangusung, P. S.  , Nlewadim, A. A.  , Uka, A.
Published: May 2024

The efficiency of four different hormonal preparations (Heterotis niloticus pituitary extract, ovaprim, Clarias gariepinus pituitary extract and carp pituitary extract) on latency period, fecundity, fertilization, hatching, and larval survival in artificial propagation of Heterotis niloticus was investigated. Total of twelve females and twelve males earthen pond raised, 24months old broods were used. The females were divided into four groups. Each group was induced to spawn with the same hormone. Sperm from one male was used to fertilize eggs stripped from individual females. The broods were injected with four different hormonal preparations; Treatment 1, Heterotis niloticus pituitary extract (HnPE); Treatment 2, ovaprim (Ova); Treatment 3, Clarias gariepinus pituitary extract (CgPE) and Treatment 4, CPE: all replicated thrice in a completely randomized design. The results of the experiment were subjected to analysis of variance (ANOVA) and all of them received equal treatment. Latency period was significant (p<0.05) among the treatments, as the period lasted for 10hrs, 9hrs, 12hrs and 11hrs for Trt1, Trt2, Trt3 and Trt4 respectively at a temperature of 28oC. Percentage egg fertilization among treatments was significant (p<0.05) with the highest value of 89.66% and 89.33% recorded with HnPE and Ovaprim respectively, and lowest result of 67.33% recorded with CgPE. Percentage egg hatchability among treatments was significant (p<0.05) with the highest value of 84.16% and 80.66% recorded with Ovaprim and HnPE respectively, while the lowest result of 57.33% and 59.50% was recorded with CgPE and CPE respectively. Percentage larval survival among treatments was significant (p<0.05) the survival was highest with eggs hatched with Ovaprim (68.66%) closely followed with CPE (66.66%) and HnPE (63.16%) while the lowest result of 47.33% and 49.50% was recorded with CgPE and HnPE respectively. The results obtained, indicates the possibility of achieving the effectiveness of controlled reproduction in Heterotis niloticus studied, by applying the appropriate type of hormonal stimulation. At the end of this research, there were disparities in the efficiency of the four hormonal preparations and it was evident though one (ovaprim) gave the highest reproductive indices in fecundity, fertilization, hatchability and larval survival as it can be easily accessible though expensive in affirmation with the work of Asangusung and Uka [1] than other one’s but on the average, ovaprim, Heterotis niloticus pituitary extract and CPE could successfully be depended upon for commercial propagation of Heterotis niloticus.
Amino Acid Compositions and In vitro Anti-inflammatory Properties of Cookies Produced from Wheat and Soybean Composite Flour
Authors: Alagbe Iyanu Caleb , Malomo Sunday Abiodun
Published: May 2024

Composite flours were formulated from wheat and soybean and mixed together to formulate four blends WSY 1 (100%), WSY 2 (80%-20%), WSY 3 (70%-30%), WSY 4 (60%-40%), respectively to bake cookies. The cookies were investigated for their amino acid profiles, in vitro anti-inflammatory properties and consumer acceptability of the cookies using standard methods. The amino acid profiles of the cookies were well established with high biological values (>70%) in terms of their essential, non-essential and hydrophobic amino acids while glutamic acid served as the most abundant amino acid (19.15 – 28.69%) present in each of the cookies. Moreso, the in-vitro anti-inflammatory properties of the cookies showed more potency with low IC50 (~20 \(\mu\)g/ml) than the standard sodium diclofenac, a well-known non-steroidal anti-inflammatory drug. Overall, the cookie sample from the 60% wheat and 40% soybean composite flour was acceptable to the consumers as shown by their ratings and perceptions, which could be highly found useful as potential bioactive antioxidant, anti-diabetic and anti-inflammatory agents in the management of chronic inflammations.
A Polyethylene Glycol‐Crosslinked Serum Albumin/Hyaluronan Hydrogel for the Cultivation of Chondrogenic Cell Types
Authors: Benz Karin , Freudigmann Christian , Müller Jana , Wurst Helmut , Albrecht Dirk , Badke Andreas , Gaissmaier Christoph , Mollenhauer Jürgen
Published: September 2010

Hydrogels are gaining increasing interest as a structural basis for regenerative cell implants. Here we present in vitro data on growth and differentiation of human mesenchymal stromal precursor cells (MSCs), adult human knee chondrocytes, and adult human intervertebral disk cells in a gel based on PEG‐crosslinked serum albumin, supplemented with 4% hyaluronan (HSA–HA). Each gel culture was compared to its parallel proliferating monolayer (PML) culture and non‐proliferating high density monolayer culture (HDML). MSCs were chondrogenically induced in both standard micromass (MM) cultures and HSA–HA. Cell viability in HSA–HA was above 90%. Chondrogenic differentiation was defined by changes in the expression of the mRNA for collagen types I, II, and X, for aggrecan, for alkaline phosphatase (ALP), and for hyaluronan synthases‐2 and ‐3 (has‐2, has‐3). For disk cells and chondrocytes, the changes for collagen type II and aggrecan were in the range of up to 100‐fold, depending on the human individual. After induction, MSCs differentiated equally well in MM and HSA–HA, showing increases spanning several orders of magnitude compared to uninduced monolayer (ML) cells. For has‐2 and has‐3, the changes were less obvious, but interestingly, the ratio of has‐2/has‐3 expression in HSA–HA cultures decreased approximately 40‐fold for disk cells, remained unchanged for chondrocytes, but increased sevenfold for MSCs, when compared to the corresponding ML cultures. Taken together, HSA–HA revealed its potential as an anchoring hydrogel for chondrogenic cell types.
Hybrids of Magnetic Nanoparticles with Double‐Hydrophilic Core/Shell Cylindrical Polymer Brushes and Their Alignment in a Magnetic Field
Authors: Xu Youyong , Yuan Jiayin , Fang Bing , Drechsler Markus , Müllner Markus , Bolisetty Sreenath , Ballauff Matthias , Müller Axel H. E.
Published: September 2010

The synthesis of double‐hydrophilic core/shell cylindrical polymer brushes (CPBs), their hybrids with magnetite nanoparticles, and the directed alignment of these magnetic hybrid cylinders by a magnetic field are demonstrated. Consecutive grafting from a polyinitiator poly(2‐(2‐bromoisobutyryloxy)ethyl methacrylate) (PBIEM) of tert‐butyl methacrylate (tBMA) and oligo(ethylene glycol) methacrylate (OEGMA) using atom‐transfer radical polymerization (ATRP) and further de‐protection yields core/shell CPBs with poly(methacrylic acid) (PMAA) as the core and POEGMA as the shell, which is evidenced by 1H NMR, gel permeation chromatography (GPC), and dynamic and static light scattering (DLS and SLS). The resulting core/shell brush is well soluble in water and shows a pH responsiveness because of its weak polyelectrolyte core. Pearl‐necklace structures are observed by cryogenic transmission electron microscopy (cryo‐TEM) at pH 4, while at pH 7, these structures disappear owing to the ionization of the core. A similar morphology is also found for the polychelate of the core/shell CPBs with Fe3+ ions. Superparamagnetic magnetite nanoparticles have also been prepared and introduced into the core of the brushes. The hybrid material retains the superparamagnetic property of the magnetite nanoparticles, which is verified by superconducting quantum interference device (SQUID) magnetization measurements. Large‐scale alignment of the hybrid cylinders in relatively low magnetic fields (40–300 mT) can easily be performed when deposited on a surface. which is clearly revealed by the atomic force microscopy (AFM) and TEM measurements.
Single Nucleotide‐Catalyzed Biomimetic Reductive Amination
Authors: Kumar Atul , Sharma Siddharth , Maurya Ram Awatar
Published: September 2010

We have successfully developed a single nucleotide (adenosine 5′‐diphosphate)‐catalyzed enantioselective direct reductive amination of aldehydes and ketones using a Hantzsch ester as reducing agent. The process is a simple, efficient and a real mimic of the NADH reduction approach for the synthesis of structurally diverse amines. This reaction is the first report demonstrating the ability of a single nucleotide as catalyst and one of the most genuine biomimetic reactions of organic chemistry.
Synthesis, in‐vitro Cytotoxicity, and a Preliminary Structure‐Activity Relationship Investigation of Pyrimido[4,5‐c]quinolin‐1(2H)‐ones
Authors: Metwally Kamel , Khalil Ashraf , Pratsinis Harris , Kletsas Dimitris
Published: September 2010

As part of our ongoing research effort to develop new antimitotic agents based on the recently reported pyrimido[4,5‐c]quinoline‐1(2H)‐one ring skeleton, we were interested in identifying structural elements that contribute to the cytotoxicity of this class of compounds. The effect of several quinoline‐ring substituents was examined and the new compounds were evaluated in vitro for cytotoxicity against three human cancer cell lines namely, lung fibrosarcoma HT‐1080, colon adenocarcinoma HT‐29, and breast carcinoma MDA‐MB‐231. Most of the compounds showed cytotoxic activity in the low micromolar and sub‐micromolar range. Structure‐activity relationship information revealed that a combination of electronic and steric factors may be involved. Flow cytometric cell cycle analysis performed on HT‐1080 cells revealed that the most cytotoxic compounds 48, 50, 54, 59, and 63 inhibit the S‐phase and arrest the cells in the G2/M phase of the cell cycle suggesting an antimitotic action of these compounds.
Synthesis of New Quinoline Derivatives as Inhibitors of Human Tumor Cells Growth
Authors: Rashad Aymn E. , El‐Sayed Wael A. , Mohamed Ashraf M. , Ali Mamdouh M.
Published: September 2010

A series of new 8‐[(2H‐tetrazol‐5‐yl)methoxy]quinoline derivatives, their sugar hydrazones, and their N‐glycoside derivatives were synthesized. Furthermore, the 1,2,4‐triazole‐3‐one derivatives 3 and 4 were synthesized from the amidrazone derivative 2. Some of the newly prepared compounds demonstrated inhibitory effects on the growth of MCF‐7 human breast cancer cells as compared with the activity of the commonly used anticancer drug, cisplatin. The results of antitumor evaluation revealed that compounds 2–5, 8b, and 12 inhibited the growth of cancer cells through their effect as free‐radical regulators by increasing the activity of superoxide dismutase and depletion of intracellular levels of reduced glutathione, catalase and glutathione peroxidase activities, accompanied with a high production of hydrogen peroxide, nitric oxide, and other free radicals causing the killing of tumor cells. The results suggested that the prepared compounds possess significant anticancer activity comparable to cisplatin and the antitumor activity of these prepared compounds was accompanied with a reduction in the levels of protein and nucleic acids.
Synthese [b]‐kondensierter azepindione durch dealkoxycarbonylierung
Authors: Kunick Conrad
Published: September 2010

Synthesis of [b]‐Fused Azepinediones by Dealkoxycarbonylation

The 2,3,4,5‐tetrahydro‐1H‐1‐benzazepinediones 2 and 5,6,7,8‐tetrahydro‐4H‐thieno[3,2‐b]azepine‐5,8‐dione (11) are obtained by dealkoxycarbonylation in wet dimethylsulfoxide from fused azepinecarboxylic esters 3 and 10, respectively, 3b and 10 are prepared by Dieckmann reaction with KH in DMF/toluene from diesters 6 and 9, respectively.
Karstic morphologies identified with geophysics around Saulges caves (Mayenne, France)
Authors: Valois Rémi , Bermejo Lucia , Guérin Roger , Hinguant Stéphan , Pigeaud Romain , Rodet Joël
Published: September 2010

Geophysical measurements were carried out around Saulges caves that sought to highlight the local karstic morphologies and the impact on preservation of archaeological material within the caves. Electrical resistivity tomographies (ERTs) and apparent conductivity mapping detected a soil cover on the plateaus that ends abruptly over fractured limestone or over a bowl‐shaped structure filled with clay soil. Moreover, there is at least one zone of soil accumulation with a basin form with almost no soil cover around this structure. The ERT and seismic refraction tomography (SRT) detected an important anomaly in the valley. Many clues indicate that this anomaly is a karstic conduit filled with water or clay. Therefore, some karstic dissolution zones have been found and only geophysical methods are able to detect such features. Detection of preferential pathways could help to protect prehistoric art within the caves and new karstic morphologies help to better understand this karstic system. Copyright © 2010 John Wiley & Sons, Ltd.
Geophysical prospection of the frontiers of the Roman Empire in southern Germany, UNESCO World Heritage Site
Authors: Fassbinder Jorg W. E.
Published: September 2010

The Roman Limes with a length of 550 km is the largest archaeological site of Europe as well as the largest monument of the Roman period. In July 2005 it was decided that the Limes and its interrelated archaeological sites, together with Hadrian's Wall in England, would be a component of a ‘Trans‐National World Heritage Site’ taking the name ‘Frontiers of the Roman Empire’. From that point it was necessary to minimize and/or to avoid archaeological excavation. Further research on such sites is mainly limited to the application of non‐destructive techniques. Among other geophysical tools, magnetometry, based on the rock magnetic knowledge turned out to be a highly suitable method. Two examples that allowed verification and completion of old maps of the Reichs‐Limes‐Kommission will be shown here; these projects exemplify geophysical work on the Bavarian Limes. At the site of Oberhochstatt we discovered the exact location and determined information on the size and orientation of the fort that previous searches for a long time had failed to find. At Theilenhofen we were able to complete the map of the whole fort with all fortification ditches and the water supply, to verify the troop level and to confirm the former fort on which is superimposed the traces of the Roman vicus. Copyright © 2010 John Wiley & Sons, Ltd.
Mitochondria and the culture of the Borg
Authors: Braschi Emelie , McBride Heidi M.
Published: September 2010

As endosymbionts, the mitochondria are unique among organelles. This review provides insights into mitochondrial behavior and introduces the idea of a unified collective, an interconnected reticulum reminiscent of the Borg, a fictional humanoid species from the Star Trek television series whereby decisions are made within their network (or “hive”), linked to signaling cascades that coordinate the cross‐talk between mitochondrial and cellular processes (“subspace domain”). Similarly, mitochondrial dynamics are determined by two distinct processes, namely the local regulation of fission/fusion and the global control of their behavior through cellular signaling pathways. Indeed, decisions within the hive provide each mitochondrial unit with autonomous control of their own degradation, whereby mitochondrial fusion is inactivated and they become substrates for autophagy. Decisions within the subspace domain couple signaling pathways involved in the functional integration of mitochondria with complex cellular transitions, including developmental cues, mitosis, and apoptosis.
Primary rat hepatocyte culture on 3D nanofibrous polymer scaffolds for toxicology and pharmaceutical research
Authors: Bierwolf Jeanette , Lutgehetmann Marc , Feng Kai , Erbes Johannes , Deichmann Steffen , Toronyi Eva , Stieglitz Christina , Nashan Bjoern , Ma Peter X. , Pollok Joerg M.
Published: September 2010

Primary rat hepatocytes are a widely used experimental model to estimate drug metabolism and toxicity. In currently used two‐dimensional (2D) cell culture systems, typical problems like morphological changes and the loss of liver cell‐specific functions occur. We hypothesize that the use of polymer scaffolds could overcome these problems and support the establishment of three‐dimensional (3D) culture systems in pharmaceutical research. Isolated primary rat hepatocytes were cultured on collagen‐coated nanofibrous scaffolds for 7 days. Cell loading efficiency was quantified via DNA content measurement. Cell viability and presence of liver‐cell‐specific functions (albumin secretion, glycogen storage capacity) were evaluated. The activity of liver‐specific factors was analyzed by immunofluorescent staining. RNA was isolated to establish quantitative real‐time PCR. Our results indicate that primary rat hepatocytes cultured on nanofibrous scaffolds revealed high viability and well‐preserved glycogen storage. Albumin secretion was existent during the entire culture period. Hepatocytes remain HNF‐4 positive, indicating highly preserved cell differentiation. Aggregated hepatocytes re‐established positive signaling for Connexin 32, a marker for differentiated hepatocyte interaction. ZO‐1‐positive hepatocytes were detected indicating formation of tight junctions. Expression of cytochrome isoenzymes was inducible. Altogether the data suggest that nanofibrous scaffolds provide a good in vitro microenvironment for neo tissue regeneration of primary rat hepatocytes. Biotechnol. Bioeng. 2011; 108:141–150. © 2010 Wiley Periodicals, Inc.
High‐throughput screening of a small molecule library for promoters and inhibitors of mesenchymal stem cell osteogenic differentiation
Authors: Brey Darren M. , Motlekar Nuzhat A. , Diamond Scott L. , Mauck Robert L. , Garino Jonathon P. , Burdick Jason A.
Published: September 2010

The use of high‐throughput screening (HTS) techniques has long been employed by the pharmaceutical industry to increase discovery rates for new drugs that could be useful for disease treatment, yet this technology has only been minimally applied in other applications such as in tissue regeneration. In this work, an assay for the osteogenic differentiation of human mesenchymal stem cells (hMSCs) was developed and used to screen a library of small molecules for their potential as either promoters or inhibitors of osteogenesis, based on levels of alkaline phosphatase activity and cellular viability. From a library of 1,040 molecules, 36 promoters, and 20 inhibitors were identified as hits based on statistical criteria. Osteopromoters from this library were further investigated using standard culture techniques and a wider range of outcomes to verify that these compounds drive cellular differentiation. Several hits led to some improvement in the expression of alkaline phosphatase, osteogenic gene expression, and matrix mineralization by hMSCs when compared to the standard dexamethasone supplemented media and one molecule was investigated in combination with a recently identified biodegradable and osteoconductive polymer. This work illustrates the ability of HTS to more rapidly identify potential molecules to control stem cell differentiation. Biotechnol. Bioeng. 2011; 108:163–174. © 2010 Wiley Periodicals, Inc.
The analysis of glycosylation: a continued need for high pH anion exchange chromatography
Authors: Behan Jennifer L. , Smith Kevin D.
Published: September 2010

An appreciation of the structures of the oligosaccharide chains which become attached to biomolecules (the process known as glycosylation), and their relevance to the biological function of the molecule concerned, has progressed rapidly in recent years with developments in site‐selective protein glycosylation, oligosaccharide synthesis and in vivo targeting of oligosaccharides. These developments have necessitated the parallel development of effective analytical tools for the determination of the structures of glycosylation. The conclusion of studies in the 1980s and 1990s was that high pH anion exchange chromatography (HPAEC) was the most effective HPLC mode for the analysis of glycosylation. It allowed the fractionation of complex mixtures of monosaccharides or oligosaccharides, the latter in terms of charge, size, composition, anomerity and intra‐chain linkages. This review reinvestigates whether HPAEC still appears to offer the most effective means of analysing glycosylation. Copyright © 2010 John Wiley & Sons, Ltd.
Description of a UK study of onset of sexually harmful behaviour before the age of ten years in boys referred to a specialist assessment and treatment service
Authors: Hawkes Colin
Published: September 2010

During the past 30 years, research into the phenomenon of sexually harmful behaviour has shifted from an adult focus to include adolescent and, more recently, childhood onset. This paper reports a study of onset, before the age of ten years, of sexually harmful behaviour in a group of 27 boys.

Ethical consent was obtained to extract information from the case files of a national specialist service. A description of characteristics was built up from variable frequencies and quantitative and qualitative analysis produced findings that indicated a family history of cross‐generational harm to children and a parental experience of unresolved harm in childhood generated inconsistent and insensitive parenting that was linked to high levels of maltreatment and insecurity of attachment in the research group. Sexualised reactions by the research subjects to a very high level of sexual victimisation were not responded to in a timely or appropriate way by parents, other caregivers or professionals so that sexually harmful behaviour continued without intervention for a significant period.

The study proposes a three‐stage model that identifies predisposing vulnerabilities, sexual victimisation as a trigger event and the subsequent development of sexually harmful behaviour as a protecting adaptation that assumes an aroused and organised aspect through repetition. Copyright © 2010 John Wiley & Sons, Ltd.
Stress‐induced multiple organ damage in rats is ameliorated by the antioxidant and anxiolytic effects of regular exercise
Authors: Çakır Barış , Kasımay Özgür , Kolgazi Meltem , Ersoy Yasemin , Ercan Feriha , Yeğen Berrak Ç.
Published: September 2010

Our aim was to investigate the effects of moderate load, regular swimming exercise on stress‐induced anxiety, and associated oxidative organ injury. Male Sprague‐Dawley rats (n = 48) were either kept sedentary or submitted to swimming exercise for 8 weeks. Rats were then divided as non‐stressed, acute stress, and chronic stress groups. After acute or chronic stress (electric foot shocks) applications, rats were placed on a holeboard and the exploratory behavior was recorded to assess the anxiety. Rats were decapitated after the stress application. Acute and chronic stress induction led to increased serum cortisol levels as compared to non‐stressed groups. Plasma aspartate aminotransferase levels that were elevated in sedentary rats with both stress exposures were lower in trained rats. Malondialdehyde levels and myeloperoxidase activity were increased in the cardiac muscle, liver, stomach, and brain of the stressed rats with a concomitant reduction in the glutathione levels, while stress‐induced changes in malondialdehyde, myeloperoxidase, and glutathione levels were reversed in the trained animals. Exercise, which led to increased malondialdehyde and reduced glutathione levels in the skeletal muscle of the non‐stressed rats, also protected against stress‐induced oxidative damage. Regular exercise with its anxiolytic and antioxidant effects ameliorates stress‐induced oxidative organ damage by a neutrophil‐dependent mechanism. Copyright © 2010 John Wiley & Sons, Ltd.
Intercatheter reproducibility of near‐infrared spectroscopy for the in vivo detection of coronary lipid core plaques
Authors: Abdel‐Karim Abdul‐Rahman R. , Rangan Bavana V. , Banerjee Subhash , Brilakis Emmanouil S.
Published: September 2010

Objectives:

To evaluate the intercatheter reproducibility of catheter‐based intracoronary near‐infrared spectroscopy (NIRS) for the detection of coronary lipid core plaques (LCPs).

Background:

The intercatheter in vivo reproducibility of coronary NIRS findings has not been evaluated.

Methods:

NIRS assessment using an automated pullback catheter was performed in triplicates in 10 patients using two different NIRS catheters to evaluate the reproducibility of the lipid core burden index (LCBI).

Results:

Mean age was 62 ± 7 years, and all patients were men with high prevalence of hypertension (90%), hyperlipidemia (90%), diabetes (50%), and current smoking (50%). The mean LCBI measured by the first and second catheters was 64 ± 55 and 69 ± 47, respectively (intraclass correlation coefficient, 0.97; 95% confidence interval, 0.90–0.99; and Spearman's ρ, 0.95; P < 0.001 for both). The mean LCBI measured again with the second catheter was 71 ± 63 (intraclass correlation coefficient, 0.96; 95% confidence interval, 0.84–0.99; and Spearman's ρ, 0.95; P < 0.001 for both). The mean number of LCPs detected by the first and second catheters was 1.9 ± 1.2 and 2.0 ± 1.5, respectively (intraclass correlation coefficient, 0.75, P = 0.005 and Spearman's ρ, 0.74, P = 0.01). The mean number of LCPs at reimaging with the second catheter was 2.0 ± 1.76 (intraclass correlation coefficient, 0.96 and Spearman's ρ, 0.98; P < 0.001 for both).

Conclusion:

NIRS measurements of LCP with two different catheters have high reproducibility. The findings support the use of NIRS to assess the status of LCP in patients over time and to assess LCP changes in response to novel treatments. © 2011 Wiley‐Liss, Inc.
Left atrial appendage closure with amplatzer cardiac plug in atrial fibrillation: Initial european experience
Authors: Park Jai‐Wun , Bethencourt Armando , Sievert Horst , Santoro Gennaro , Meier Bernhard , Walsh Kevin , Lopez‐Minquez Jose Ramon , Meerkin David , Valdés Mariano , Ormerod Oliver , Leithäuser Boris
Published: September 2010

Background: In most patients with atrial fibrillation (AF) and stroke, there is thrombotic embolization from the left atrial appendage (LAA). Percutaneous closure of the LAA is a novel alternative for the treatment of patients with AF at a high risk of stroke, in whom long‐term anticoagulation therapy is not possible or not desired. This study details the initial experience with the Amplatzer Cardiac Plug (ACP) in humans. Methods: Investigator‐initiated retrospective preregistry data collection to evaluate procedural feasibility and safety up to 24 hr after implantation of the ACP, a nitinol device designed for percutaneous trans‐septal implantation in LAA of patients with paroxysmal, permanent, or persistent AF. Results: In 137 of 143 patients, LAA occlusion was attempted, and successfully performed in 132 (96%). There were serious complications in 10 (7.0%) patients (three patients with ischemic stroke; two patients experienced device embolization, both percutaneously recaptured; and five patients with clinically significant pericardial effusions). Minor complications were insignificant pericardial effusions in four, transient myocardial ischemia in two, and loss of the implant in the venous system in one patient. Conclusion: The implantation of the ACP device is a feasible method for percutaneous occlusion of the LAA. © 2011 Wiley‐Liss, Inc.
Photosensitive Surfactants with Various Hydrophobic Tail Lengths for the Photocontrol of Genomic DNA Conformation with Improved Efficiency
Authors: Diguet Antoine , Mani Naresh Kumar , Geoffroy Marie , Sollogoub Matthieu , Baigl Damien
Published: September 2010

We report the synthesis and characterisation of photosensitive cationic surfactants with various hydrophobic tail lengths. These molecules, called AzoCx, are used as photosensitive nucleic acid binders (pNABs) and are applied to the photocontrol of DNA conformation. All these molecules induce DNA compaction in a photodependent way, originating in the photodependent polarity of their hydrophobic tails. We show that increasing hydrophobicity strongly enhances the compaction efficiencies of these molecules, but reduces the possibility of reversible photocontrol of a DNA conformation. Optimal performance was achieved with AzoC5, which allowed reversible control of DNA conformation with light at a concentration seven times smaller than previously reported.
ChemInform Abstract: Crystal Structure of CsHg5Cl11.
Authors: PAKHOMOV V. I. , GORYUNOV A. V.
Published: September 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.