bluuebunny/crossref_metadata_2025 huggingface dataset:
Climate-induced flood inundation for the Arial Khan River of Bangladesh using open-source SWAT and HEC-RAS model for RCP8.5-SSP5 scenario
Authors: Roy Binata , Khan Md. Sabbir Mostafa , Islam A. K. M. Saiful , Mohammed Khaled , Khan Md. Jamal Uddin
Published: May 2021

Bangladesh is one of the largest flood-prone deltas of the GBM (Ganges–Brahmaputra–Meghna) basins, and recently, it is categorized as the 7th worst climate-affected country in the world. Future climate change along with economic development, urbanization, and increase in population may worsen this situation manifolds. To cope with future flood situations and lessen probable flood losses, it is essential to develop flood maps of the major flood-prone rivers of Bangladesh considering climate change scenarios. In this study, the flood inundation of the Arial Khan River and its floodplain has been assessed for the predicted climate change scenario of RCP 8.5 (Representative Concentration Pathway 8.5) using open-source mathematical models. A calibrated and validated hydrologic model of GBM basins in SWAT (Soil and Water Assessment Tool) model has been used to estimate the future flow magnitudes at Bahadurabad Transit (Brahmaputra River) and Hardinge Bridge (Ganges River) using extreme emission scenario RCP 8.5. Using the flow magnitude of these two stations as the upstream boundaries, an HEC-RAS 1D model has been set up for the Brahmaputra, Ganges, and Padma rivers for generating future flow magnitude at the offtake of the Arial Khan River. Later, an HEC-RAS 1D-2D coupled model is set up for the Arial Khan River floodplain and flood maps are prepared considering flood depth, duration, and inundation extent. The flood assessment for different projections of RCP 8.5 shows that there is an increasing trend of flood in terms of depth, duration, and inundation from the 2020s to the 2080s. Hence, the floodplain becomes more hazardous by the end of this century. The climate change impact on the projected population for the RCP 8.5 scenario is assessed under SSP5 (Shared Socioeconomic Pathways 5) which indicates that the total flood-affected population will be nearly twice in the 2080s compared to the 2020s. So, future climate change is going to have a dreadful effect on the flood situation of the Arial Khan River floodplain.
Distributed Intelligence in the Internet of Things: Challenges and Opportunities
Authors: Alsboui Tariq , Qin Yongrui , Hill Richard , Al-Aqrabi Hussain
Published: May 2021

Widespread adoption of smart IoT devices is accelerating research for new techniques to make IoT applications secure, scalable, energy-efficient, and capable of working in mission-critical use cases, which require an ability to function offline. In this context, the novel combination of distributed ledger technology (DLT) and distributed intelligence (DI) is seen as a practical route towards the decentralisation of IoT architectures. This paper surveys DI techniques in IoT and commences by briefly explaining the need for DI, by proposing a comprehensive taxonomy of DI in IoT. This taxonomy is then used to review existing techniques and to investigate current challenges that require careful attention and consideration. Based on the taxonomy, IoT DI techniques can be classified into five categories based on the factors that support distributed functionality and data acquisition: cloud-computing, mist-computing, distributed-ledger-technology, service-oriented-computing and hybrid. Existing techniques are compared and categorized mainly based on related challenges, and the level of intelligence supported. We evaluate more than thirty current research efforts in this area. We define many significant functionalities that should be supported by DI frameworks and solutions. Our work assists system architects and developers to select the correct low-level communication techniques in an integrated IoT-to-DLT-to-cloud system architecture. The benefits and shortcomings of different DI approaches are presented, which will inspire future work into automatic hybridization and adaptation of DI mechanisms. Finally, open research issues for distributed intelligence in IoT are discussed.
Innovation Pathways in the NHS: An Introductory Review
Authors: Arora Anmol , Wright Andrew , Cheng Tsz Kin Mark , Khwaja Zahra , Seah Matthew
Published: May 2021

Healthcare as an industry is recognised as one of the most innovative. Despite heavy regulation, there is substantial scope for new technologies and care models to not only boost patient outcomes but to do so at reduced cost to healthcare systems and consumers. Promoting innovation within national health systems such as the National Health Service (NHS) in the United Kingdom (UK) has been set as a key target for health care professionals and policy makers. However, while the UK has a world-class biomedical research industry, several reports in the last twenty years have highlighted the difficulties faced by the NHS in encouraging and adopting innovations, with the journey from idea to implementation of health technology often taking years and being very expensive, with a high failure rate. This has led to the establishment of several innovation pathways within and around the NHS, to encourage the invention, development and implementation of cost-effective technologies that improve health care delivery. These pathways span local, regional and national health infrastructure. They operate at different stages of the innovation pipeline, with their scope and work defined by location, technology area or industry sector, based on the specific problem identified when they were set up. In this introductory review, we outline each of the major innovation pathways operating at local, regional and national levels across the NHS, including their history, governance, operating procedures and areas of expertise. The extent to which innovation pathways address current challenges faced by innovators is discussed, as well as areas for improvement and future study.
Using data to make the case for program resources and sustainability: the BEST action inventory case study
Authors: Rojewski Julie M. W. , Ayala-Lopez Nadia , Nguyen Sean , Watts Stephanie W.
Published: May 2021

Career development programs are a valuable part of any student’s experience, and increasingly is an expected part of graduate school training. While such programs are commonly available to undergraduates, there is a growing need for career support to be offered to graduate students. Making the case for resources can be a challenge in this domain, however. Research on the impact of career services for graduate students and post-doctoral scholars is a growing scholarly concern. However, there remains a need to better understand what level of intervention is most appropriate: What kind of activities, how much time, and what resources would best serve the professional development needs of graduate students and post-doctoral scholars? And to answer these questions, a more foundational one: what activities are drawing the attention of graduate students and post doctoral trainees, and in what activities are they spending their time? In this manuscript, we describe how Our University approached this research question by developing an online data tracking system to capture graduate and post-doctoral trainee participation in one co-curricular professional development program. We demonstrate how this data tracking system can be used to advocate for institutional resources in career development programming, for research, and for practical purposes such as advocating for institutional support and for program design and assessment.
Thermodynamics of smooth models of pseudo–Anosov homeomorphisms
Authors: VECONI DOMINIC
Published: May 2021

We develop a thermodynamic formalism for a smooth realization of pseudo-Anosov surface homeomorphisms. In this realization, the singularities of the pseudo-Anosov map are assumed to be fixed, and the trajectories are slowed down so the differential is the identity at these points. Using Young towers, we prove existence and uniqueness of equilibrium states for geometric t-potentials. This family of equilibrium states includes a unique SRB measure and a measure of maximal entropy, the latter of which has exponential decay of correlations and the central limit theorem.
Hegel's Concept of the Familiar: Toward a Philosophical Study
Authors: Aldouri Hammam
Published: May 2021

One of the most memorable lines of Hegel's oeuvre is from the preface to his Phenomenology of Spirit: ‘Quite generally, the familiar, just because it is familiar, is not cognitively understood.’ Surprisingly, relatively little philosophical attention has been paid to the notion of ‘the familiar’ in Hegel scholarship. This essay aims to rectify this lack by offering a preliminary inquiry in what the notion means across Hegel's work. It does so by focusing on three underexplored moments in Hegel's work: (1) the exposition of the logic of appearance in the description and diagnosis of seventeenth and eighteenth century Dutch painting in the lecture notes from the 1823 Berlin lectures on the philosophy of fine art; (2) the function of institutionalized practice in Hegel's Tübingen essay of 1793; and (3) the conjunctural relation of the familiar with philosophical terminology in an entry from the ‘Aphorisms from the Wastebook.’ Through a careful reading of these three moments, I will show that the familiar is a point that constellates multiple processes, mechanisms and apparatuses that, when grasped in their complex totality, functions as the ‘prius’ (priority/prioritization/first moment) of Hegel's speculative philosophy.
Race, Reconstruction, and the Invention of “Negro Superstition,” 1862–1877
Authors: COX DAVID G.
Published: May 2021

This article traces the postbellum development and dissemination of the notion of “negro superstition.” By the end of Reconstruction, many whites across the nation, both liberal and conservative, shared in the belief that credulity was the keystone of African American culture. The formulation of superstition as innate racial trait served the conjoined causes of sectional reconciliation and white supremacy, eroding white support for black citizenship. As liberal estimations of black Christianity declined and conservative depictions of African American magical beliefs proliferated, “voodoo” gained traction as a potent imaginary, shorthand for racial atavism, unreason, and dangerous sexuality.
Code-switching in parents’ everyday speech to bilingual infants
Authors: KREMIN Lena V. , ALVES Julia , ORENA Adriel John , POLKA Linda , BYERS-HEINLEIN Krista
Published: May 2021

Code-switching is a common phenomenon in bilingual communities, but little is known about bilingual parents’ code-switching when speaking to their infants. In a pre-registered study, we identified instances of code-switching in day-long at-home audio recordings of 21 French–English bilingual families in Montreal, Canada, who provided recordings when their infant was 10 and 18 months old. Overall, rates of infant-directed code-switching were low, averaging 7 times per hour (6 times per 1,000 words) at 10 months and increasing to 28 times per hour (18 times per 1,000 words) at 18 months. Parents code-switched more between sentences than within a sentence; this pattern was even more pronounced when infants were 18 months than when they were 10 months. The most common apparent reasons for code-switching were to bolster their infant's understanding and to teach vocabulary words. Combined, these results suggest that bilingual parents code-switch in ways that support successful bilingual language acquisition.
Solving equations in dense Sidon sets
Authors: PRENDIVILLE SEAN
Published: May 2021

We offer an alternative proof of a result of Conlon, Fox, Sudakov and Zhao [CFSZ20] on solving translation-invariant linear equations in dense Sidon sets. Our proof generalises to equations in more than five variables and yields effective bounds.
Finnish inserted vowels: a case of phonologized excrescence
Authors: Karlin Robin
Published: May 2021

In this paper, I examine a case of vowel insertion found in Savo and Pohjanmaa dialects of Finnish that is typically called “epenthesis”, but which demonstrates characteristics of both phonetic excrescence and phonological epenthesis. Based on a phonological analysis paired with an acoustic corpus study, I argue that Finnish vowel insertion is the mixed result of phonetic excrescence and the phonologization of these vowels, and is related to second-mora lengthening, another dialectal phenomenon. I propose a gestural model of second-mora lengthening that would generate vowel insertion in its original phonetic state. The link to second-mora lengthening provides a unified account that addresses both the dialectal and phonological distribution of the phenomenon, which have not been linked in previous literature.
The ‘Other’ gumēzišn. About the Final ‘Merger’ of Limited Time with Eternity
Authors: PANAINO ANTONIO
Published: May 2021

The Middle Persian (MP) verb gumēxtan, gumēz- [gwmyhtn', gymyc-], despite its Indo-Iranian and Indo-European origins, does not offer attestations in Avestan and Old Persian. This is a very peculiar fact, because in particular a nominal derivative of this verbal stem, gumēzišn [gwmyck'], became one of the most sensible technical terms adopted in the framework of the Mazdean cosmology and theology. We can briefly recall that the gumēzišn concerns the ‘state of mixture’, the mélange produced by the irruption of Ahreman in the primordial good creation of Ohrmazd, when he pierced the heavens and entered the world.
Evaluation of the Causes of Wet‐Season Dry Biases Over Amazonia in CAM5
Authors: Ma Hsi‐Yen , Zhang Kai , Tang Shuaiqi , Xie Shaocheng , Fu Rong
Published: May 2021

This study investigates the causes of pronounced low precipitation bias over Amazonia in the Community Atmosphere Model version 5 (CAM5), a common feature in many global climate models. Our analysis is based on a suite of 3‐day long hindcasts starting every day at 00Z from 1997 to 2012 and an AMIP simulation for the same period. The Amazonia dry bias appears by the second day in the hindcasts and is very robust for all the seasons with the largest bias magnitude during the wet season (December–February). The bias pattern and magnitude do not change much during different dynamical wind regimes on sub‐seasonal time scales. We further classify the diurnal cycle of precipitation near the LBA sites from observations and hindcasts into three convective regimes: no precipitation, late afternoon deep convection, and nighttime deep convection. CAM5 can only simulate the late afternoon convective regime and completely fails to simulate the nighttime convection, which is mostly from propagating convective systems originating from remote locations. CAM5 mainly underestimates precipitation in the late afternoon and nighttime convective regimes, which occur during ∼67% of wet season days and account for ∼75% of accumulated precipitation amount in observations. The persistent warm temperature bias and slightly higher moisture below 850 mb likely trigger deep convection too frequently, resulting in an earlier but weaker rainfall peak in the diurnal cycle. Furthermore, shallow convection may not effectively transport moisture from boundary layer to the free atmosphere, which also leads to weaker deep convection events.
Wind: Discoveries and Impacts of a Venerable Spacecraft
Authors: Wilson III Lynn
Published: May 2021

Wind has been one of the most robust, diverse, long-lasting, and impactful heliophysics missions ever to have been carried out.
Anthony Brian Watts Receives 2020 Maurice Ewing Medal
Authors:   AGU
Published: May 2021

Anthony Brian Watts was awarded the 2020 Maurice Ewing Medal at the virtual AGU Fall Meeting in December. The medal is for “significant original contributions to the ocean sciences.”
Growth outcomes of small for gestational age preterm infants before and after implementation of an exclusive human milk-based diet
Authors: Fleig Lindsay , Hagan Joseph , Lee Martin L. , Abrams Steven A. , Hawthorne Keli M. , Hair Amy B.
Published: May 2021

Objective

Small for gestational age (SGA) preterm infants (PT) are at greatest risk for growth failure. Our objective was to assess the impact of an exclusive human milk diet (HUM) on growth velocities and neonatal morbidities from birth to discharge in a SGA population.

Study design

Multicenter, retrospective cohort study, subgroup analysis of SGA PT comparing a cow’s milk diet (CMD) with HUM diet.

Results

At birth 420 PT were classified as SGA (197 CMD group, 223 HUM group). Demographics and anthropometric measurements were similar. HUM group PT showed improvement in length Z score at discharge (p = 0.024) and reduction in necrotizing enterocolitis (NEC) (p = 0.004).

Conclusion

SGA PT fed a HUM diet had significantly decreased incidence of NEC, surgical NEC, and late-onset sepsis. Due to concerns about growth in a HUM diet, it is reassuring SGA infants fed the HUM diet had similar growth to CMD diet with trends toward improvement.
Dynamics of neutralizing antibody responses to SARS-CoV-2 in patients with COVID-19: an observational study
Authors: Xu Xin , Nie Sheng , Wang Yanqun , Long Quanxin , Zhu Hong , Zhang Xiaoyong , Sun Jian , Zeng Qinglang , Zhao Jincun , Liu Li , Li Ling , Huang Ailong , Hou Jinlin , Hou Fan Fan
Published: May 2021

Our understanding of the protective immunity, particularly the long-term dynamics of neutralizing antibody (NAbs) response to SARS-CoV-2, is currently limited. We enrolled a cohort of 545 COVID-19 patients from Hubei, China, who were followed up up to 7 months, and determined the dynamics of NAbs to SARS-CoV-2 by using a surrogate virus neutralization test (sVNT). In our validation study, sVNT IC50 titers and the neutralization rate measured at a single dilution (1:20) were well correlated with FRNT titers (r = 0.85 and 0.84, respectively). The median time to seroconversion of NAbs was 5.5 days post onset of symptoms. The rate of positive sVNT was 52% in the first week, reached 100% in the third week, and remained above 97% till 6 months post onset. Quantitatively, NAbs peaked in the fourth week and only a quarter of patients had an estimated peak titer of >1000. NAbs declined with a half-time of 61 days (95% CI: 49–80 days) within the first two months, and the decay deaccelerated to a half-time of 104 days (95% CI: 86–130 days) afterward. The peak levels of NAbs were positively associated with severity of COVID-19 and age, while negatively associated with serum albumin levels. The observation that the low-moderate peak neutralizing activity and fast decay of NAbs in most naturally infected individuals called for caution in evaluating the feasibility of antibody-based therapy and vaccine durability. NAbs response positively correlated with disease severity, warning for the possibility of repeat infection in patients with mild COVID-19.
Extinction of anciently associated gut bacterial symbionts in a clade of stingless bees
Authors: Cerqueira Alan Emanuel Silva , Hammer Tobin J , Moran Nancy A , Santana Weyder Cristiano , Kasuya Maria Catarina Megumi , da Silva Cynthia Canêdo
Published: May 2021

Animal-microbe symbioses are often stable for millions of years. An example is the clade consisting of social corbiculate bees—honeybees, bumblebees, and stingless bees—in which a shared ancestor acquired specialized gut bacteria that subsequently diversified with hosts. This model may be incomplete, however, as few microbiomes have been characterized for stingless bees, which are diverse and ecologically dominant pollinators in the tropics. We surveyed gut microbiomes of Brazilian stingless bees, focusing on the genus Melipona, for which we sampled multiple species and biomes. Strikingly, Melipona lacks Snodgrassella and Gilliamella, bacterial symbionts ubiquitous in other social corbiculate bees. Instead, Melipona species harbor more environmental bacteria and bee-specific Starmerella yeasts. Loss of Snodgrassella and Gilliamella may stem from ecological shifts in Melipona or the acquisition of new symbionts as functional replacements. Our findings demonstrate the value of broadly sampling microbiome biodiversity and show that even ancient symbioses can be lost.
Pharmacogenetic genotype and phenotype frequencies in a large Danish population-based case-cohort sample
Authors: Lunenburg Carin A. T. C. , Thirstrup Janne P. , Bybjerg-Grauholm Jonas , Bækvad-Hansen Marie , Hougaard David M. , Nordentoft Merete , Werge Thomas , Børglum Anders D. , Mors Ole , Mortensen Preben B. , Gasse Christiane
Published: May 2021

Pharmacogenetics aims to improve clinical care by studying the relationship between genetic variation and variable drug response. Large population-based datasets could improve our current understanding of pharmacogenetics from selected study populations. We provide real-world pharmacogenetic frequencies of genotypes and (combined) phenotypes of a large Danish population-based case-cohort sample (iPSYCH2012; data of the Integrative Psychiatric Research consortium). The genotyped sample consists of 77,684 individuals, of which 51,464 individuals had diagnoses of severe mental disorders (SMD case-cohort) and 26,220 were individuals randomly selected from the Danish population (population cohort). Array-based genotype data imputed to 8.4 million genetic variants was searched for a selected pharmacogenetic panel of 42 clinically relevant variants and a CYP2D6 gene deletion and duplication. We identified 19 of 42 variants. Minor allele frequencies (MAFs) were consistent with previously reported MAFs, and did not differ between SMD cases and population cohorts. Almost all individuals carried at least one genetic variant (> 99.9%) and 87% carried three or more genetic variants. When genotypes were translated into phenotypes, also > 99.9% of individuals had at least one divergent phenotype (i.e. divergent from the common phenotypes considered normal, e.g. extensive metabolizer). The high number of identified individuals with at least one pharmacogenetic variant or divergent phenotype indicates the importance of pharmacogenetic panel-based genotyping. Combined CYP2C19-CYP2D6 phenotypes revealed that 72.7% of individuals had divergent phenotypes for one or both enzymes. As CYP2D6 and CYP2C19 have an important role in the metabolism of psychotropic drugs, this indicates the relevance of pharmacogenetic testing specifically in individuals using psychotropic drugs.
The protective role of MC1R in chromosome stability and centromeric integrity in melanocytes
Authors: Li Xin , Mao Weiwei , Chen Jie , Goding Colin R. , Cui Rutao , Xu Zhi-Xiang , Miao Xiao
Published: May 2021

Variants in the melanocortin-1 receptor (MC1R) gene, encoding a trimeric G-protein-coupled receptor and activated by α-melanocyte-stimulating hormone (α-MSH), are frequently associated with red or blonde hair, fair skin, freckling, and skin sensitivity to ultraviolet (UV) light. Several red hair color variants ofMC1Rare also associated with increased melanoma risk.MC1Rvariants affect melanoma risk independent of phenotype. Here, we demonstrated that MC1R is a critical factor in chromosome stability and centromere integrity in melanocytes. α-MSH/MC1R stimulation prevents melanocytes from UV radiation-induced damage of chromosome stability and centromere integrity. Mechanistic studies indicated that α-MSH/MC1R-controlled chromosome stability and centromeric integrity are mediated by microphthalmia-associated transcription factor (Mitf), a transcript factor needed for the α-MSH/MC1R signaling and a regulator in melanocyte development, viability, and pigment production. Mitf directly interacts with centromere proteins A in melanocytes. Given the connection amongMC1Rvariants, red hair/fair skin phenotype, and melanoma development, these studies will help answer a question with clinical relevance “why red-haired individuals are so prone to developing melanoma”, and will lead to the identification of novel preventive and therapeutic strategies for melanomas, especially those with redheads.
Smoothened inhibition leads to decreased cell proliferation and suppressed tissue fibrosis in the development of benign prostatic hyperplasia
Authors: Liu Jianmin , Yin Jing , Chen Ping , Liu Daoquan , He Weixiang , Li Yan , Li Mingzhou , Fu Xun , Zeng Guang , Guo Yuming , Wang Xinghuan , DiSanto Michael E. , Zhang Xinhua
Published: May 2021

Benign prostatic hyperplasia (BPH) is a common disease in aging males. It has been proven that the Hedgehog (HH) is implied as an effective and fundamental regulatory growth factor signal for organogenesis, homeostasis, and regeneration. Smoothened (SMO), as the major control point of HH signals, activates aberrantly in most human solid tumors. However, the specific function of SMO and its downstream glioma-associated oncogene (GLI) family in BPH has not been well understood. Here, we first revealed that the SMO cascade was upregulated in BPH tissues and was localized in both the stromal and the epithelium compartments of human prostate tissues. Cyclopamine, as a specific SMO inhibitor, was incubated with BPH-1 and WPMY-1, and intraperitoneally injected into a BPH rat model established by castration with testosterone supplementation. SMO inhibition could induce cell apoptosis, cell cycle arrest at the G0/G1 phase, and a reduction of tissue fibrosis markers, both in vitro and in vivo. Finally, a tissue microarray, containing 104 BPH specimens, was constructed to analyze the correlations between the expression of SMO cascade and clinical parameters. The GLI2 was correlated positively with nocturia and negatively with fPSA. The GLI3 was in a positive relationship with International Prostate Symptom Score and nocturia. In conclusion, our study suggested that SMO cascade could play important roles in the development of BPH and it might be rediscovered as a promising therapeutic target for BPH.
Economic damages from Hurricane Sandy attributable to sea level rise caused by anthropogenic climate change
Authors: Strauss Benjamin H. , Orton Philip M. , Bittermann Klaus , Buchanan Maya K. , Gilford Daniel M. , Kopp Robert E. , Kulp Scott , Massey Chris , Moel Hans de , Vinogradov Sergey
Published: May 2021

In 2012, Hurricane Sandy hit the East Coast of the United States, creating widespread coastal flooding and over $60 billion in reported economic damage. The potential influence of climate change on the storm itself has been debated, but sea level rise driven by anthropogenic climate change more clearly contributed to damages. To quantify this effect, here we simulate water levels and damage both as they occurred and as they would have occurred across a range of lower sea levels corresponding to different estimates of attributable sea level rise. We find that approximately $8.1B ($4.7B–$14.0B, 5th–95th percentiles) of Sandy’s damages are attributable to climate-mediated anthropogenic sea level rise, as is extension of the flood area to affect 71 (40–131) thousand additional people. The same general approach demonstrated here may be applied to impact assessments for other past and future coastal storms.
Nuclear organisation and replication timing are coupled through RIF1–PP1 interaction
Authors: Gnan Stefano , Flyamer Ilya M. , Klein Kyle N. , Castelli Eleonora , Rapp Alexander , Maiser Andreas , Chen Naiming , Weber Patrick , Enervald Elin , Cardoso M. Cristina , Bickmore Wendy A. , Gilbert David M. , Buonomo Sara C. B.
Published: May 2021

Three-dimensional genome organisation and replication timing are known to be correlated, however, it remains unknown whether nuclear architecture overall plays an instructive role in the replication-timing programme and, if so, how. Here we demonstrate that RIF1 is a molecular hub that co-regulates both processes. Both nuclear organisation and replication timing depend upon the interaction between RIF1 and PP1. However, whereas nuclear architecture requires the full complement of RIF1 and its interaction with PP1, replication timing is not sensitive to RIF1 dosage. The role of RIF1 in replication timing also extends beyond its interaction with PP1. Availing of this separation-of-function approach, we have therefore identified in RIF1 dual function the molecular bases of the co-dependency of the replication-timing programme and nuclear architecture.
A luminescent view of the clickable assembly of LnF3 nanoclusters
Authors: Zhou Jie , Wei Yang , Pan Yue , Wang Yue , Yuan Ze , Zhang Fan , Song Hao , Yue Jingyi , Su Haiquan , Xie Xiaoji , Huang Ling
Published: May 2021

Nanoclusters (NCs) bridge the gap between atoms and nanomaterials in not only dimension but also physicochemical properties. Precise chemical and structural control, as well as clear understanding of formation mechanisms, have been important to fabricate NCs with high performance in optoelectronics, catalysis, nanoalloys, and energy conversion and harvesting. Herein, taking advantage of the close chemical properties of Ln3+ (Ln = Eu, Nd, Sm, Gd, etc.) and Gd3+–Eu3+ energy transfer ion-pair, we report a clickable LnF3 nanoparticle assembly strategy allowing reliable fabrication of diversely structured NCs, including single-component, dimeric, core-shelled/core-shell-shelled, and reversely core-shelled/core-shell-shelled, particularly with synergized optical functionalities. Moreover, the purposely-embedded dual luminescent probes offer great superiority for in situ and precise tracking of tiny structural variations and energy transfer pathways within complex nanoarchitectures.
Electrically connected spin-torque oscillators array for 2.4 GHz WiFi band transmission and energy harvesting
Authors: Sharma Raghav , Mishra Rahul , Ngo Tung , Guo Yong-Xin , Fukami Shunsuke , Sato Hideo , Ohno Hideo , Yang Hyunsoo
Published: May 2021

The mutual synchronization of spin-torque oscillators (STOs) is critical for communication, energy harvesting and neuromorphic applications. Short range magnetic coupling-based synchronization has spatial restrictions (few µm), whereas the long-range electrical synchronization using vortex STOs has limited frequency responses in hundreds MHz (<500 MHz), restricting them for on-chip GHz-range applications. Here, we demonstrate electrical synchronization of four non-vortex uniformly-magnetized STOs using a single common current source in both parallel and series configurations at 2.4 GHz band, resolving the frequency-area quandary for designing STO based on-chip communication systems. Under injection locking, synchronized STOs demonstrate an excellent time-domain stability and substantially improved phase noise performance. By integrating the electrically connected eight STOs, we demonstrate the battery-free energy-harvesting system by utilizing the wireless radio-frequency energy to power electronic devices such as LEDs. Our results highlight the significance of electrical topology (series vs. parallel) while designing an on-chip STOs system.
A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
Authors: Xun Guanhua , Lane Stephan Thomas , Petrov Vassily Andrew , Pepa Brandon Elliott , Zhao Huimin
Published: May 2021

The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.
RAS mutations drive proliferative chronic myelomonocytic leukemia via a KMT2A-PLK1 axis
Authors: Carr Ryan M. , Vorobyev Denis , Lasho Terra , Marks David L. , Tolosa Ezequiel J. , Vedder Alexis , Almada Luciana L. , Yurcheko Andrey , Padioleau Ismael , Alver Bonnie , Coltro Giacomo , Binder Moritz , Safgren Stephanie L. , Horn Isaac , You Xiaona , Solary Eric , Balasis Maria E. , Berger Kurt , Hiebert James , Witzig Thomas , Buradkar Ajinkya , Graf Temeida , Valent Peter , Mangaonkar Abhishek A. , Robertson Keith D. , Howard Matthew T. , Kaufmann Scott H. , Pin Christopher , Fernandez-Zapico Martin E. , Geissler Klaus , Droin Nathalie , Padron Eric , Zhang Jing , Nikolaev Sergey , Patnaik Mrinal M.
Published: May 2021

Proliferative chronic myelomonocytic leukemia (pCMML), an aggressive CMML subtype, is associated with dismal outcomes. RAS pathway mutations, mainly NRASG12D, define the pCMML phenotype as demonstrated by our exome sequencing, progenitor colony assays and a Vav-Cre-NrasG12D mouse model. Further, these mutations promote CMML transformation to acute myeloid leukemia. Using a multiomics platform and biochemical and molecular studies we show that in pCMML RAS pathway mutations are associated with a unique gene expression profile enriched in mitotic kinases such as polo-like kinase 1 (PLK1). PLK1 transcript levels are shown to be regulated by an unmutated lysine methyl-transferase (KMT2A) resulting in increased promoter monomethylation of lysine 4 of histone 3. Pharmacologic inhibition of PLK1 in RAS mutant patient-derived xenografts, demonstrates the utility of personalized biomarker-driven therapeutics in pCMML.
Functional and structural characterization of a two-MAb cocktail for delayed treatment of enterovirus D68 infections
Authors: Zhang Chao , Xu Cong , Dai Wenlong , Wang Yifan , Liu Zhi , Zhang Xueyang , Wang Xuesong , Wang Haikun , Gong Sitang , Cong Yao , Huang Zhong
Published: May 2021

Enterovirus D68 (EV-D68) is an emerging pathogen associated with respiratory diseases and/or acute flaccid myelitis. Here, two MAbs, 2H12 and 8F12, raised against EV-D68 virus-like particle (VLP), show distinct preference in binding VLP and virion and in neutralizing different EV-D68 strains. A combination of 2H12 and 8F12 exhibits balanced and potent neutralization effects and confers broader protection in mice than single MAbs when given at onset of symptoms. Cryo-EM structures of EV-D68 virion complexed with 2H12 or 8F12 show that both antibodies bind to the canyon region of the virion, creating steric hindrance for sialic acid receptor binding. Additionally, 2H12 binding can impair virion integrity and trigger premature viral uncoating. We also capture an uncoating intermediate induced by 2H12 binding, not previously described for picornaviruses. Our study elucidates the structural basis and neutralizing mechanisms of the 2H12 and 8F12 MAbs and supports further development of the 2H12/8F12 cocktail as a broad-spectrum therapeutic agent against EV-D68 infections in humans.
Global effects of land-use intensity on local pollinator biodiversity
Authors: Millard Joseph , Outhwaite Charlotte L. , Kinnersley Robyn , Freeman Robin , Gregory Richard D. , Adedoja Opeyemi , Gavini Sabrina , Kioko Esther , Kuhlmann Michael , Ollerton Jeff , Ren Zong-Xin , Newbold Tim
Published: May 2021

Pollinating species are in decline globally, with land use an important driver. However, most of the evidence on which these claims are made is patchy, based on studies with low taxonomic and geographic representativeness. Here, we model the effect of land-use type and intensity on global pollinator biodiversity, using a local-scale database covering 303 studies, 12,170 sites, and 4502 pollinating species. Relative to a primary vegetation baseline, we show that low levels of intensity can have beneficial effects on pollinator biodiversity. Within most anthropogenic land-use types however, increasing intensity is associated with significant reductions, particularly in urban (43% richness and 62% abundance reduction compared to the least intensive urban sites), and pasture (75% abundance reduction) areas. We further show that on cropland, the strongly negative response to intensity is restricted to tropical areas, and that the direction and magnitude of response differs among taxonomic groups. Our findings confirm widespread effects of land-use intensity on pollinators, most significantly in the tropics, where land use is predicted to change rapidly.
Interaction-induced topological phase transition and Majorana edge states in low-dimensional orbital-selective Mott insulators
Authors: Herbrych J. , Środa M. , Alvarez G. , Mierzejewski M. , Dagotto E.
Published: May 2021

Topological phases of matter are among the most intriguing research directions in Condensed Matter Physics. It is known that superconductivity induced on a topological insulator’s surface can lead to exotic Majorana modes, the main ingredient of many proposed quantum computation schemes. In this context, the iron-based high critical temperature superconductors are a promising platform to host such an exotic phenomenon in real condensed-matter compounds. The Coulomb interaction is commonly believed to be vital for the magnetic and superconducting properties of these systems. This work bridges these two perspectives and shows that the Coulomb interaction can also drive a canonical superconductor with orbital degrees of freedom into the topological state. Namely, we show that above a critical value of the Hubbard interaction the system simultaneously develops spiral spin order, a highly unusual triplet amplitude in superconductivity, and, remarkably, Majorana fermions at the edges of the system.
Climate risks and adaptation strategies of farmers in East Africa and South Asia
Authors: Aryal Jeetendra Prakash , Sapkota Tek Bahadur , Rahut Dil Bahadur , Marenya Paswel , Stirling Clare M.
Published: May 2021

Understanding major climate risks, adaptation strategies, and factors influencing the choice of those strategies is crucial to reduce farmers’ vulnerability. Employing comprehensive data from 2822 farm households in Ethiopia and Kenya (East Africa; EA) and 1902 farm households in Bangladesh, India, and Nepal (South Asia; SA), this study investigates the main climate risks that farmers faced and the adaptation strategies they used. Among others, excessive rainfall and heightened crop pest/disease incidence are commonly observed climate-induced risks in all study areas, while cyclones and salinity are unique to Bangladesh. Drought is prevalent in Ethiopia, India, Kenya, and Nepal. Farmers in those countries responded with strategies that include change in farming practices, sustainable land management, reduce consumption, sell assets, use savings and borrowings, seek alternative employment and assistance from government or NGO. In general, farmers faced several multiple climate risks simultaneously and they responded with multiple adaptation strategies. Therefore, this study used a multivariate probit (MVP) approach to examine the factors influencing the adoption of adaptation strategies. Unlike other studies, we also tested and corrected for possible endogeneity in model estimation. All the countries mentioned have low adaptive capacity to address climate change, which is further weakened by inadequate governance and inefficient institutions. We observed significant differences in the choice of adaptation strategies between male-headed households (MHHs) and female-headed households (FHHs), as well as across countries. Generally, MHHs are more likely to seek additional employment and change agricultural practices, while FHHs and households headed by older persons tend to reduce consumption and rely on savings and borrowings. Institutional support for adaptation is much less in EA compared to SA. Training on alternative farming practices, enhancing non-farm employment options, better institutional support, and social security for older farmers are crucial for climate change adaptation in both regions.
Effect of COVID-19 on liver abnormalities: a systematic review and meta‐analysis
Authors: Bzeizi Khalid , Abdulla Maheeba , Mohammed Nafeesa , Alqamish Jehad , Jamshidi Negar , Broering Dieter
Published: May 2021

Emerging evidence suggest association of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection with the development of many liver abnormalities. The overarching aim of this study was therefore to assess the available evidence on the clinical effects of SARS-CoV-2 on the profiles of liver chemistries and coagulation in COVID-19 diagnosed patients. We considered all study designs including epidemiological and observational that reported liver function test abnormalities in patients confirmed with SARS-CoV-2 infection. Medline, Embase databases and Google Scholar as well as relevant reviews were searched to identify appropriate studies from inception to 31st of August 2020. We calculated the pooled mean with 95% confidence intervals (95% CI) through a random-effect model meta-analysis. A total of 35 studies with 10,692 participants were considered for the review from which 23 studies with sufficient quantitative data were included in the meta-analysis. The pooled mean for liver enzymes and coagulation parameters did not significantly change in patients diagnosed with COVID-19 and remained within normal range. Notwithstanding potential bias from confounding factors in interpretation of data in this review, findings from the observational studies and case reports suggest that COVID-19 does not appear to have a significant impact on the transaminases or total bilirubin levels of patients with confirmed SARS-CoV-2 infection. Further controlled studies and larger sample size observational studies are needed with adequate reporting of other liver function parameters are warranted.
ATP hydrolysis and nucleotide exit enhance maltose translocation in the MalFGK2E importer
Authors: Abreu Bárbara , Cruz Carlos , Oliveira A. Sofia F. , Soares Cláudio M.
Published: May 2021

ATP binding cassette (ABC) transporters employ ATP hydrolysis to harness substrate translocation across membranes. The Escherichia coli MalFGK2E maltose importer is an example of a type I ABC importer and a model system for this class of ABC transporters. The MalFGK2E importer is responsible for the intake of malto-oligossacharides in E.coli. Despite being extensively studied, little is known about the effect of ATP hydrolysis and nucleotide exit on substrate transport. In this work, we studied this phenomenon using extensive molecular dynamics simulations (MD) along with potential of mean force calculations of maltose transport across the pore, in the pre-hydrolysis, post-hydrolysis and nucleotide-free states. We concluded that ATP hydrolysis and nucleotide exit trigger conformational changes that result in the decrease of energetic barriers to maltose translocation towards the cytoplasm, with a concomitant increase of the energy barrier in the periplasmic side of the pore, contributing for the irreversibility of the process. We also identified key residues that aid in positioning and orientation of maltose, as well as a novel binding pocket for maltose in MalG. Additionally, ATP hydrolysis leads to conformations similar to the nucleotide-free state. This study shows the contribution of ATP hydrolysis and nucleotide exit in the transport cycle, shedding light on ABC type I importer mechanisms.
The role of tunable nonlinear dark resonances on vacuum Rabi splitting and optical bistability in an atom-cavity system
Authors: Hui SiJia , Wen Feng , Zhang Minghui , Zhang ShaoWei , Yang YuanJie , Dai ZhiPing , Su YungPeng , Zhang YanPeng , Wang HongXing
Published: May 2021

The phenomenon of “dark resonances” is a well-known concept in quantum optics and laser spectroscopy. As a general rule, interactions involving in such a “dark state” lead to multiple quantum superposition states that interact coherently and are undesirable. In this paper, two types nonlinear interaction in an atomic cavity, namely the nested and cascaded interactions, are theoretically analyzed how the dark resonances form the dark state peak to modulate the vacuum Rabi splitting (VRS) and optical bistability (OB) behavior. In both the zero- and high order modes, there are four VRS peaks generated in the nested interaction and three in the cascade interaction. Dark resonance can modulate not only the peak number of VRS, but also the OB thresholds. It is found that dark state can determine the asymmetric OB distribution of nested type and symmetric OB distribution of cascade type. Besides that, the distinctive OB thresholds in two kinds of interaction also be studied. The observations not only conceptually extend the conventional “dark resonances” phenomenon, but also opens the door for a variety of new applications in tunable all-optical switch and quantum communication.
Altered endothelial dysfunction-related miRs in plasma from ME/CFS patients
Authors: Blauensteiner J. , Bertinat R. , León L. E. , Riederer M. , Sepúlveda N. , Westermeier F.
Published: May 2021

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disease characterized by unexplained debilitating fatigue. Although the etiology is unknown, evidence supports immunological abnormalities, such as persistent inflammation and immune-cell activation, in a subset of patients. Since the interplay between inflammation and vascular alterations is well-established in other diseases, endothelial dysfunction has emerged as another player in ME/CFS pathogenesis. Endothelial nitric oxide synthase (eNOS) generates nitric oxide (NO) that maintains endothelial homeostasis. eNOS is activated by silent information regulator 1 (Sirt1), an anti-inflammatory protein. Despite its relevance, no study has addressed the Sirt1/eNOS axis in ME/CFS. The interest in circulating microRNAs (miRs) as potential biomarkers in ME/CFS has increased in recent years. Accordingly, we analyze a set of miRs reported to modulate the Sirt1/eNOS axis using plasma from ME/CFS patients. Our results show that miR-21, miR-34a, miR-92a, miR-126, and miR-200c are jointly increased in ME/CFS patients compared to healthy controls. A similar finding was obtained when analyzing public miR data on peripheral blood mononuclear cells. Bioinformatics analysis shows that endothelial function-related signaling pathways are associated with these miRs, including oxidative stress and oxygen regulation. Interestingly, histone deacetylase 1, a protein responsible for epigenetic regulations, represented the most relevant node within the network. In conclusion, our study provides a basis to find endothelial dysfunction-related biomarkers and explore novel targets in ME/CFS.