bluuebunny/crossref_metadata_2025 huggingface dataset:
A Survey of Energy Optimization Approaches for Computational Task Offloading and Resource Allocation in MEC Networks
Authors: Yang Jinming , Shah Awais Aziz , Pezaros Dimitrios
Published: August 2023

With the increased penetration of cloud computing and virtualization, a plethora of internet of things devices have been deployed globally. As a result, computationally intensive tasks are transmitted from the edge towards the centralized cloud for processing that leads to increased energy utilization in the cloud data centers while at the same increasing significant latency for critical applications. Recent years have witnessed a paradigm shift from centralized cloud computing towards mobile edge computing (MEC), where computational tasks are offloaded at the edge servers near user equipment (UE). This paradigm leads to lowering the energy utilization in the cloud data centers, along with low latency for UE and efficient resource utilization at the edge. In this context, the scale and complexity of the MEC networks is drastically increasing and, consequently, finding effective energy-efficient solutions for computational task offloading and resource allocation in MEC networks has become an ambitious task. To address the aforementioned challenges, this work surveys the state of the art in different categorizations of algorithm-based computational task offloading and resource allocation strategies focusing on energy utilization. It also provides a detailed cross-comparison of existing strategies in terms of their implementation specifications. Additionally, this paper also highlights open challenges and potential future research directions to facilitate efficient task offloading and resource allocation at the edge with reduced energy consumption at the centralized data centers. Our work also paves the way for the deployment of critical applications at the edge that require low latency and high service quality guarantees.
Semantic-Enhanced Cross-Modal Fusion for Improved Unsupervised Image Captioning
Authors: Xiang Nan , Chen Ling , Liang Leiyan , Rao Xingdi , Gong Zehao
Published: August 2023

Unsupervised image captioning often grapples with challenges such as image–text mismatches and modality gaps, resulting in suboptimal captions. This paper introduces a semantic-enhanced cross-modal fusion model (SCFM) to address these issues. The SCFM integrates three innovative components: a text semantic enhancement network (TSE-Net) for nuanced semantic representation; contrast learning for optimizing similarity measures between text and images; and enhanced visual selection decoding (EVSD) for precise captioning. Unlike existing methods that struggle with capturing accurate semantic relationships and flexibility across scenarios, the proposed model provides a robust solution for unbiased and diverse captioning. Through experimental evaluations on the MS COCO and Flickr30k datasets, SCFM demonstrates significant improvements over the benchmark model, enhancing the CIDEr and BLEU-4 metrics by 3.6% and 3.2%, respectively. Visualization analysis further reveals the model’s superiority in increasing variability between hidden features and its potential in cross-domain and stylized image captioning. The findings not only contribute to the advancement of image captioning techniques but also open avenues for future research. Further investigations will explore SCFM’s adaptability to other multimodal tasks and refine it for more intricate image–text relationships.
Review of Methods for Diagnosing the Degradation Process in Power Units Cooperating with Renewable Energy Sources Using Artificial Intelligence
Authors: Ziółkowski Paweł , Drosińska-Komor Marta , Głuch Jerzy , Breńkacz Łukasz
Published: August 2023

This work is based on a literature review (191). It mainly refers to two diagnostic methods based on artificial intelligence. This review presents new possibilities for using genetic algorithms (GAs) for diagnostic purposes in power plants transitioning to cooperation with renewable energy sources (RESs). The genetic method is rarely used directly in the modeling of thermal-flow analysis. However, this assignment proves that the method can be successfully used for diagnostic purposes. The GA method was presented in this work for thermal-flow studies of steam turbines controlled from the central power system to obtain the stability of RESs. It should be remembered that the development of software using genetic algorithms to locate one-off degradations is necessary for a turbine that works sustainably with RESs. In this paper, against the background of the review, diagnostic procedures create an inverse model of a thermal power plant. Algorithms were used to detect fast global extremes through the convergence of simulated signatures with signs explaining degradation. In addition, statistical dependencies are used in the selection phase to accelerate fault detection. The created procedure allows obtaining a diagnosis in the form of a single degradation. This procedure turns out to be quite effective for the above example.
Variable Water Flow Control of Hybrid Geothermal Heat Pump System
Authors: Shin Ji-Hyun , Kim Hyo-Jun , Lee Han-Gyeol , Cho Young-Hum
Published: August 2023

Ground heat accumulation caused by imbalanced heating and cooling loads in a building can cause the heat-source temperature to increase as the operating age of a geothermal heat pump (GHP) system increases. An alternative system to improve upon this situation is the hybrid GHP system. This study reviews existing research on GHP systems and hybrid GHP systems, variable water flow (VWF) control, and coefficient of performance (COP) prediction. Generally, constant flow control is applied to the circulating pump to provide a flow rate according to the maximum load. The need for VWF control was identified because the hybrid GHP system is used mainly as a heating and cooling heat source system for partial loads rather than the entire building load. Previous studies on predicting the COPs of GHP systems developed prediction models by selecting input values based on mathematical models, collecting data through multiple measurement points, and utilizing data from production environments. The model can be limited by the field environment, and it is necessary to predict the COP using machine learning based on existing field monitoring data.
Automated Generation of Energy Profiles for Urban Simulations
Authors: Maile Tobias , Steinacker Heiner , Stickel Matthias W. , Ott Etienne , Kley Christian
Published: August 2023

Urban simulations play an important role on the way to a climate neutral society. To enable early assessment of different energy concepts for urban developments, energy profiles for different building types are needed. This work describes the development and use of a new engineering tool GenSim to quickly and reliably generate energy profiles for urban simulations and early building energy predictions. While GenSim is a standalone tool to create energy profiles for early design assessment, it was developed in the context of urban simulations to primarily support energy efficient urban developments within Germany. Energy engineers quickly embraced the tool due to its simplicity and comprehensible results. The development of the tool was recently switched to open source to enable its usage to a broader audience. In order to foster its development and use, a detailed testing framework has been established to ensure the quality of the results of the tool. The paper includes a detailed validation section to demonstrate the validity of the results compared to a detailed building energy simulation model and actual measured performance data.
A New Perspective on Hydrogen Chloride Scavenging at High Temperatures for Reducing the Smoke Acidity of PVC Cables in Fires V: Comparison between EN 60754-1 and EN 60754-2
Authors: Bassi Iacopo , Bandinelli Claudia , Delchiaro Francesca , Piana Marco , Sarti Gianluca
Published: August 2023

Regulation (EU) No 305/2011 lays down harmonized conditions for marketing construction products in the European Union. One of its consequences has been the introduction of the product standard EN 50575 and standard EN 130501-6, concerning power, control, and communication cables permanently installed in buildings to prevent the risk of a fire and its consequences. EN 13501-6 provides the reaction to fire classifications for cables, the test methods to be performed, the requirements to meet a specific reaction to fire, and additional classifications for smoke production, flaming droplets, and acidity. It requires EN 60754-2 as the technical standard to assess acidity, and it defines three classes: a1, a2, and a3 (the less performant). Due to the release of hydrogen chloride during the combustion, acidity is the weak point of PVC cables, which are not yet capable of achieving the a1 or a2 classes required for specific locations according to fire risk assessments. EN 13501-6 does not include EN 60754-1, used in harmonized standards outside the scope of Regulation (EU) No 305/2011. EN 60754-1 and EN 60754-2 are common standards for determining halogen gas content, and acidity/conductivity, respectively. While they involve the same type of test apparatus, they differ in heating regimes, final temperatures, and detection methods. In particular, EN 60754-2 requires testing at temperatures between 935–965 °C in the tube furnace, where the sample burns, the smoke is collected in bubblers, and pH and conductivity are measured as an indirect assessment of acidity. On the other hand, the temperature regime of EN 60754-1 is a gradual heating run, followed by isothermal heating at 800 °C. The paper shows that when potent acid scavengers are used in PVC compounds, performing EN 60754-2 with the thermal profile of EN 60754-1 or at 500 °C in isothermal conditions, the evolution of hydrogen chloride changes significantly up to 10 times less than the test performed in isothermal at 950 °C. The reason lies behind the kinetic of hydrogen chloride release during the combustion of PVC compounds: the higher the temperature or faster the heat release, the quicker hydrogen chloride evolution and the lower the probability for the acid scavenger to trap it. Thus, these findings emphasize the “fragility” of EN 60754-2 as a tool for assessing risks associated with the release of hydrogen chloride during fires.
Physicochemical Quality Retention during Cold Storage of Prepackaged Barramundi Meat Processed with a New Microwave-Assisted Induction Heating Technology
Authors: Hwang Chiu-Chu , Chien Hung-I , Lee Yi-Chen , Kao Jun-Cheng , Huang Yu-Ru , Huang Ya-Ling , Huang Chun-Yung , Tsai Yung-Hsiang
Published: August 2023

Microwave-assisted induction heating (MAIH) is a composite microwave and induction heating to supply rapid and uniform heating of food. A recent study showed that the optimum MAIH heating condition for barramundi meat was 90 °C/110 s or 70 °C/130 s. This study examines whether the microwave-assisted induction heating (MAIH) technology (at 70 °C for 130 s or 90 °C for 110 s) can more effectively slow down the quality loss of barramundi meat during cold storage than the traditional boiling method (at 90 °C for 150 s). The results show that no microbial growth was observed in the three groups of heated barramundi meat samples during the 60 days of cold storage. However, the MAIH technology slowed down the increase in the total volatile basic nitrogen (TVBN) content more significantly than the boiling method. As the cold storage time increased, though, the L* (lightness), a* (redness), and W (whiteness) values decreased, while the b* (yellowness) and color difference (ΔE) values increased in the three treatment groups. However, the MAIH technology slowed down the decrease in the L*, a*, and W values more significantly, and produced a ΔE value smaller than the boiling method. Moreover, the MAIH technology ensured higher hardness and chewiness of the barramundi meat than the boiling method. Overall, the MAIH technology slowed down the quality loss of the barramundi meat and maintained better color and texture during cold storage.
Pesticide Residues and Berry Microbiome after Ozonated Water Washing in Table Grape Storage
Authors: Caponio Gabriele , Vendemia Marco , Mallardi Domenica , Marsico Antonio Domenico , Alba Vittorio , Gentilesco Giovanni , Forte Giovanna , Velasco Riccardo , Coletta Antonio
Published: August 2023

Nowadays, different systems for reducing pesticides in table grapes are being tested at different production stages either in the field or in postharvest. The present study tested ozonated water treatments at the beginning of the cold storage of the Princess® seedless table grape variety to reduce the residue contents of some pesticides and to evaluate their effect on gray mold and the berry microbiome. An ozone generator capable of producing an ozone concentration ranging from 18 to 65 Nm3 was utilized for obtaining three ozone concentration levels in water: 3, 5 and 10 mg/L. Ozonated water was placed in a 70 L plastic box where 500 g grape samples closed in perforated plastic clamshell containers were immersed utilizing two washing times (5 and 10 min). Overall, six ozonated water treatments were tested. After the ozonated water treatments, all samples were stored for 30 days at 2 °C and 95% relative humidity to simulate commercial practices. The pesticide residue contents were determined before the ozonated water treatments (T0) and 30 days after the cold storage (T1). The treatments with ozonated water washing reduced the pesticide residues up to 100%, while the SO2 control treatment reduced the pesticide residues ranging from 20.7 to 60.7%. Using 3 mg/L ozonated water to wash grapes for 5 min represented the optimal degradation conditions for all of the analyzed pesticides, except for fludioxonil, which degraded better with a washing time of 10 min. The ozone treatments did not significantly reduce the gray mold and the fungal and bacterial microbiome, while a relevant reduction was observed in the yeast population.
Chlamydomonas agloeformis from the Ecuadorian Highlands: Nutrients and Bioactive Compounds Profiling and In Vitro Antioxidant Activity
Authors: Grande Teresa , Vornoli Andrea , Lubrano Valter , Vizzarri Francesco , Raffaelli Andrea , Gabriele Morena , Novoa Jeniffer , Sandoval Carla , Longo Vincenzo , Echeverria Maria Cristina , Pozzo Luisa
Published: August 2023

Green microalgae are single-celled eukaryotic organisms that, in recent years, are becoming increasingly important in the nutraceutical, cosmetic, and pharmaceutical fields because of their high content of bioactive compounds. In this study, a particular green microalga was isolated from freshwater highland lakes of Ecuador and morphologically and molecularly identified as Chlamydomonas agloeformis (ChA), and it was studied for nutritional and nutraceutical properties. The phenolic composition and the fatty acids profile of lyophilized cells were determined. The methanolic extract was analyzed for the phenolic compounds profile and the antioxidant capacity by means of in vitro tests. Finally, Human Microvascular Endothelial Cells (HMEC-1) were exploited to explore the capacity of ChA to reduce the endothelial damage induced by oxidized LDL-mediated oxidative stress. The extract showed a good antioxidant ability thanks to the high content in polyphenolic compounds. The observed decrease in HMEC-1 cells endothelial damage also was probably due to the antioxidant compounds present in the extract. Based on the outcomes of our in vitro assays, ChA demonstrated to be a promising source of bioactive compounds possessing exceptional antioxidant capacities which make it a prospective functional food.
The Immunomodulatory Effect of β-Glucan Depends on the Composition of the Gut Microbiota
Authors: Sung Miseon , Yoon Yohan , Lee Jeeyeon
Published: August 2023

This study aimed to elucidate the relationship between the immunomodulatory effects of β-glucan and the composition of gut microbiota in mice. The mice were fed a diet containing β-glucan for 3 weeks, and feces, blood, and tissues were then collected to analyze the immunomodulatory effect and gut microbiota composition. Based on the results of the analysis of the expression level of immune-associated proteins, the high immunomodulatory effect group (HIE) and low immunomodulatory effect group (LIE) were categorized. Before the β-glucan diet, the proportions of the phylum Bacteroidota, family Muribaculaceae, and family Lactobacillaceae were significantly higher in HIE than in LIE. Furthermore, the genus Akkermansia was absent before the β-glucan diet and increased after β-glucan diet. These microbes had the ability to metabolize β-glucan or were beneficial to health. In conclusion, our findings demonstrate that variation in the composition of gut microbiota among individuals can result in varying expressions of β-glucan functionality. This outcome supports the notion that β-glucan may be metabolized through diverse pathways by gut microbes originally possessed by mice, subsequently producing various metabolites, such as short-chain fatty acids. Alternatively, the viscosity of the intestinal mucosa could be enhanced by β-glucan, potentially promoting the growth of certain bacteria (e.g., the genus Akkermansia). This study provides insights into the intricate interplay between β-glucan, gut microbiota, and immunomodulation.
Inactivation of Foodborne Pathogens by Lactiplantibacillus Strains during Meat Fermentation: Kinetics and Mathematical Modelling
Authors: Hashemi Seyed Mohammad Bagher , Roohi Reza , Akbari Masoud , Di Natale Alessandra , Conte Francesca
Published: August 2023

This study examined the effect of beef fermentation with Lactiplantibacillus paraplantarum (L) PTCC 1965, Lactiplantibacillus (L) plantarum subsp. plantarum PTCC 1745, and Lactiplantibacillus (L) pentosus PTCC 1872 bacteria on the growth of pathogenic bacteria, including Salmonella (S) Typhi PTCC 1609 and Staphylococcus (S) aureus PTCC 1826. The growth of lactic acid bacteria (LAB) and the effect of fermentation on pathogenic bacteria were studied using Weibull: biphasic linear and competitive models. The results showed that the rate of pH reduction was lower in the early stages and increased as the microbial population grew. The α parameter was lower for L. plantarum subsp. plantarum compared to L. paraplantarum and L. pentosus. The comparison of the α parameter for bacterial growth and pH data showed that the time interval required to initiate the rapid growth phase of the bacteria was much shorter than that for the rapid pH reduction phase. The pH value had a 50% greater effect on the inactivation of S. Typhi when compared to the samples containing L. plantarum subsp. plantarum and L. pentosus. The same parameter was reported to be 72% for the inactivation of St. aureus. In general, during the fermentation process, LAB strains caused a decrease in pH, and as a result, reduced the growth of pathogens, which improves consumer health and increases the food safety of fermented meat.
Difficulties in Establishing the Adverse Effects of β-Casomorphin-7 Released from β-Casein Variants—A Review
Authors: de Vasconcelos Marta Liliane , Oliveira Luisa Maria F. S. , Hill Jeremy Paul , Vidal Ana Maria Centola
Published: August 2023

β-Casomorphin-7 (BCM-7) is a peptide released through the proteolysis of β-casein (β-CN), which is considered a bioactive peptide displaying evidence of promoting the binding and activation of the μ-opioid receptor located in various body parts, such as the gastrointestinal tract, the immune system and potentially the central nervous system. The possible effects of BCM-7 on health are a theme rising in popularity due to evidence found in several studies on the modulation of gastrointestinal proinflammatory responses that can trigger digestive symptoms, such as abdominal discomfort. With the advancement of studies, the hypothesis that there is a correlation of the possible effects of BCM-7 with the microbiota–gut–brain axis has been established. However, some studies have suggested the possibility that these adverse effects are restricted to a portion of the population, and the topic is controversial due to the small number of in vivo studies, which makes it difficult to obtain more conclusive results. In addition, a threshold of exposure to BCM-7 has not yet been established to clarify the potential of this peptide to trigger physiological responses at gastrointestinal and systemic levels. The proportion of the population that can be considered more susceptible to the effects of BCM-7 are evidenced in the literature review. The challenges of establishing the adverse effects of BCM-7 are discussed, including the importance of quantifying the BCM-7 release in the different β-CN genotypes. In summary, the reviewed literature provides plausible indications of the hypothesis of a relationship between β-CN A1/BCM-7 and adverse health effects; however, there is need for further, especially in vivo studies, to better understand and confirm the physiological effects of this peptide.
Structural and Functional Properties of Porous Corn Starch Obtained by Treating Raw Starch with AmyM
Authors: Zhang Lei , Zhong Lingli , Wang Peiwen , Zhan Lei , Yangzong Yunzhen , He Tianqi , Liu Yi , Mao Dongmei , Ye Xianfeng , Cui Zhongli , Huang Yan , Li Zhoukun
Published: August 2023

Porous starch is attracting considerable attention for its high surface area and shielding ability, properties which are useful in many food applications. In this study, native corn starch with 15, 25, and 45% degrees of hydrolysis (DH-15, DH-25, and DH-45) were prepared using a special raw starch-digesting amylase, AmyM, and their structural and functional properties were evaluated. DH-15, DH-25, and DH-45 exhibited porous surface morphologies, diverse pore size distributions and pore areas, and their adsorptive capacities were significantly enhanced by improved molecular interactions. Structural measures showed that the relative crystallinity decreased as the DH increased, while the depolymerization of starch double helix chains promoted interactions involving disordered chains, followed by chain rearrangement and the formation of sub-microcrystalline structures. In addition, DH-15, DH-25, and DH-45 displayed lower hydrolysis rates, and DH-45 showed a decreased C∞ value of 18.9% with higher resistant starch (RS) content and lower glucose release. Our results indicate that AmyM-mediated hydrolysis is an efficient pathway for the preparation of porous starches with different functionalities which can be used for a range of applications.
Functional Training and Dual-Task Training Improve the Executive Function of Older Women
Authors: Pantoja-Cardoso Alan , Aragão-Santos Jose Carlos , Santos Poliana de Jesus , Dos-Santos Ana Carolina , Silva Salviano Resende , Lima Newton Benites Carvalho , Vasconcelos Alan Bruno Silva , Fortes Leonardo de Sousa , Da Silva-Grigoletto Marzo Edir
Published: August 2023

Functional training (FT) is a type of multicomponent training with emphasis on activities of daily living that stimulate different physical capacities in only one session. Dual-task training (DTT) is a type of training that simultaneously applies cognitive and motor stimuli. We investigated the effects of sixteen weeks of FT and DTT and eight weeks of detraining on older women’s inhibitory control, working memory, and cognitive flexibility. Sixty-two older women (66.9 ± 5.4 years; 27.7 ± 3.9 kg/m2) completed a 16-week intervention program comprising the FT (n = 31) and DTT (n = 31), and 43 returned after the detraining period. We used the Stroop Color Word Color test to evaluate inhibitory control, the Corsi Block Test to assess working memory, and the Trail Making Test to evaluate cognitive flexibility. Only DTT reduced the congruent response time between the pre-test and post-test (d= −0.64; p < 0.001), with no difference between the post-test and the detraining values (d = 1.13; p < 0.001). Both groups reduced the incongruent response time between the pre-test and post-test (FT: d = −0.61; p = 0.002; DTT: d= −0.59; p = 0.002) without a difference between groups. There were no significant differences in working memory and cognitive flexibility. Sixteen weeks of FT and DTT increased the inhibitory control of older women but not the working memory and cognitive flexibility, and these effects persisted after eight weeks of detraining.
Effectiveness of a Preventative Program for Groin Pain Syndrome in Elite Youth Soccer Players: A Prospective, Randomized, Controlled, Single-Blind Study
Authors: Cotellessa Filippo , Puce Luca , Formica Matteo , May Maria Cesarina , Trompetto Carlo , Perrone Marco , Bertulessi Andrea , Anfossi Vittorio , Modenesi Roberto , Marinelli Lucio , Bragazzi Nicola Luigi , Mori Laura
Published: August 2023

Groin pain syndrome (GPS) is a prevalent issue in soccer. This study assessed the effectiveness of a new preventive protocol on GPS for youth soccer players. The protocol included targeted stretching and strengthening exercises for the adductor and core muscles from preseason to midseason. A questionnaire and two pain provocation tests were used for the evaluation. Mild GPS required positive results in at least two evaluations, while severe GPS was associated with pain incompatible with engagement in any activity confirmed by diagnostic ultrasound. Forty-two elite male athletes (aged 16.9 ± 0.7 years) participated in the study, with half of them assigned to the usual training (control group) and the remaining athletes undergoing the preventive protocol (treatment group) for 24 weeks. GPS rates were 14.3% (three diagnoses: two mild, one severe) in the treatment group and 28.6% (six diagnoses: three mild, three severe) in the control group. Toward the end of the season, three players, one from the treatment group and two from the control group had to stop playing due to severe GPS problems. In addition, one player in the control group stopped midseason. Even though the reduction in the risk of developing GPS was not significant (relative risk of 0.50 ([95%CI 0.14 to 1.74], p = 0.2759), the halved incidence of severe GPS and the increased muscle strength related to the treatment (p = 0.0277) are encouraging data for future studies.
The Effectiveness of a Smartphone Application for Tinnitus Relief
Authors: Seol Hye Yoon , Jo Mini , Moon Il Joon
Published: August 2023

Background: There has been a growing interest in the provision of smartphone- or internet-based tinnitus management. Studies have shown a positive impact of the smartphone applications on tinnitus symptoms. However, research into a relatively long-term effect of these applications on tinnitus relief as well as sound preferences has been sparse. This study explored the potential benefit of a tinnitus application in tinnitus relief over a period of six months. Methods: Twenty-two participants with subjective tinnitus were enrolled in the study. Puretone audiometry, tinnitus pitch and loudness matching, stress assessment, and questionnaires were completed at the initial visit and three and six months after the first visit. The participants used the tinnitus application for six months. Results: A significant reduction in subjective tinnitus loudness and annoyance and subjective stress level was observed. The Tinnitus Handicap Inventory scores were also significantly decreased after six months. The participants also reported high satisfaction with the application. Music and environmental sounds were the most preferred sound stimuli. Conclusions: The findings of this study demonstrate the potential benefit of the tinnitus application for tinnitus improvement.
Effect of a Tabletop Program for Training Emerging Infectious Disease Responses in Nurses at Small- to Medium-Sized Hospitals in Areas with Poor Healthcare Access
Authors: Cha Kyung-Sook , Lee Keelyong
Published: August 2023

This study developed and evaluated a tabletop program for training nurses working in small- to medium-sized hospitals in areas with poor healthcare access for emerging a tabletop program for training emerging infectious disease responses in nurses working at small- to medium-sized hospitals in areas with poor healthcare access and evaluated infectious disease responses. A tabletop program for training responses to emerging infectious diseases was provided to 29 nurses at a general hospital with <300 beds in a region without a tertiary general hospital or healthcare institution with nationally designated inpatient wards for patients with infectious diseases. The 180 min program consisted of an online theoretical lecture (Phase 1), one-on-one drills (Phase 2), and a scenario-based tabletop exercise (Phase 3). To evaluate the program’s effect, pre- and post-knowledge, awareness, and competencies related to responses to emerging infectious diseases were assessed. The mean knowledge score significantly improved from 11.41 ± 2.33 before the training to 16.69 ± 2.48 after the training (z = −4.529, p < 0.001). The mean awareness score significantly improved from 80.83 ± 11.94 before the training to 85.45 ± 7.08 after the training (z = −2.335, p = 0.020). The mean competence score significantly increased from 67.31 ± 14.75 before the training to 79.38 ± 10.39 after the training (t = −6.187, p < 0.001). The tabletop exercise program effectively enhanced the nurses’ competencies in responding to emerging infectious diseases. The training program developed in this study may be utilized in addition to a simplified theoretical lecture to train nurses to improve their competency in responding to emerging infectious diseases.
Cloning, Characterization, and Functional Analysis of EuTIL1, a Gene-Encoding Temperature-Induced Lipocalin in Eucommia ulmoides Oliv
Authors: Wu Xi , Zhao Degang
Published: August 2023

Eucommia ulmoides Oliver is a unique tertiary relict tree species in China belonging to the Eucommia family and genus. It is a traditional and precious Chinese medicinal herb with anti-tumor, antibacterial, antioxidant, anti-inflammatory, and bidirectional blood pressure regulation effects. Eucommia ulmoides mainly grows in temperate regions of China, but due to its sensitivity to low-temperatures, it is difficult to introduce into new regions. To study the role of Eucommia ulmoides lipocalin in plants. This investigation was conducted utilizing gene cloning, bioinformatics analysis, quantitative real-time polymerase chain reaction (qRT-PCR), subcellular localization, and stable genetic transformation to transfer EuTIL1 into Nicotiana tabacum Xanthi. The wild-type transgenic vector and EuTIL1 tobacco were cold-treated, and the corresponding protective enzyme activity and cold-induced gene expression levels were measured to analyze the functions of the genes. In this study, the full-length of the temperature-induced lipocalin gene (EuTIL1) cDNA was cloned from the leaves of Eucommia ulmoides using the rapid amplification of cDNA ends (RACE) method. The sequence analysis showed that the full-length cDNA of EuTIL1 was 917 bp and encodes a protein of 188 aa residues, which is a member of the Lipocalin-2 family. Subcellular localization analysis revealed that EuTIL1 was found in the plasma membrane. The transgenic tobacco lines expressing EuTIL1 under the control of the CaMV 35S promoter had increased tolerance to cold compared to wild-type (WT) plants. The average water loss rate of EuTIL1 transgenic plants was 12.4%, the average conductivity at 24 h was 55.11%, and the malondialdehyde content at 24 h was significantly lower than that of wild-type plants. The maximum soluble sugar (SS) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, and catalase activity of EuTIL1 plants after low-temperature treatment were 22.03 mg/g, 726.87 U/g, 1283.94 U/g, and 356.84 U/g, respectively, which are significantly higher than those of the wild-type. Meanwhile, in the EuTIL1 transgenic tobacco plants, the expression of the NtDREB1, NtDREB2, NtDREB4, and NtCOR15a elevated under the low-temperature treatment condition. In conclusion, our study demonstrates that EuTIL1 is a gene involved in the cold-stress response and has the potential to enhance cold tolerance in plants, providing a potential molecular basis for the study of Eucommia ulmoides introduction and serving as a candidate gene for evaluating cold-tolerant plants.
CsSE59 Encoding Invertase/Pectin Methyl Esterase Inhibitor Is a Candidate Gene Conferring the Virescent True Leaf Phenotype in Cucumber
Authors: Zhou Yuelong , Liao Liting , Liu Liu , Xiao Lingdi , Zhou Zixian , Zhou Yong , Hu Zhaoyang , Liu Shiqiang
Published: August 2023

Leaf color mutants are the ideal materials to study the regulation mechanisms of chlorophyll biosynthesis and chloroplast development or as markers for crop breeding. In this study, we identified a virescent true leaf mutant se59 from the ethyl methane sulfonate (EMS)-induced mutant lines of cucumber (Cucumis sativus L.). The se59 mutant showed normal cotyledons, but the true leaf displays light green at early growth stage, which can recover normal green later. The se59 locus was controlled by a single recessive nuclear gene. The grana stacking in the chloroplasts of se59 decreased significantly, and both the photosynthetic ability and the photosynthetic pigment contents of the se59 were significantly lower than those of wild type. The results of BSA-seq and genotyping showed that an Invertase/Pectin Methyl Esterase Inhibitor (INV/PMEI) protein encoded by CsSE59 is a candidate gene for the virescent true leaf mutant. The expression level of CsSE59 in stem, leaf and root is high. Based on the transcriptome analysis of the first true leaf of se59 mutant, the expression levels of 17 leaf color related genes changed significantly, suggesting CsSE59 may regulate virescent true leaf by interacting with some of these genes in cucumber. The identification of CsSE59 is helpful to clarify the role of INV/PMEI in chloroplast development and to understand the mechanisms of leaf color variation in cucumber.
Mitochondrial Dynamics in Neurodegenerative Diseases: Unraveling the Role of Fusion and Fission Processes
Authors: Grel Hubert , Woznica Damian , Ratajczak Katarzyna , Kalwarczyk Ewelina , Anchimowicz Julia , Switlik Weronika , Olejnik Piotr , Zielonka Piotr , Stobiecka Magdalena , Jakiela Slawomir
Published: August 2023

Neurodegenerative diseases (NDs) are a diverse group of disorders characterized by the progressive degeneration and death of neurons, leading to a range of neurological symptoms. Despite the heterogeneity of these conditions, a common denominator is the implication of mitochondrial dysfunction in their pathogenesis. Mitochondria play a crucial role in creating biomolecules, providing energy through adenosine triphosphate (ATP) generated by oxidative phosphorylation (OXPHOS), and producing reactive oxygen species (ROS). When they’re not functioning correctly, becoming fragmented and losing their membrane potential, they contribute to these diseases. In this review, we explore how mitochondria fuse and undergo fission, especially in the context of NDs. We discuss the genetic and protein mutations linked to these diseases and how they impact mitochondrial dynamics. We also look at the key regulatory proteins in fusion (MFN1, MFN2, and OPA1) and fission (DRP1 and FIS1), including their post-translational modifications. Furthermore, we highlight potential drugs that can influence mitochondrial dynamics. By unpacking these complex processes, we aim to direct research towards treatments that can improve life quality for people with these challenging conditions.
Ecological Interactions of Predatory Mites, Cheyletus eruditus (Schrank) (Trombidiformes: Cheyletidae) and Cheyletus malaccensis Oudemans, and Prey, Liposcelis decolor (Pearman) (Psocodea: Liposcelididae), under Different Thermo-Hygrometric Regimes
Authors: Danso James K. , Opit George P. , Giles Kristopher L. , Noden Bruce H.
Published: August 2023

Predator–prey interactions are linked through trophic relationships, and individual population dynamics are a function of multiple interactions among many ecological factors. The present study considered the efficacy of the predatory mites Cheyletus eruditus (Schrank) (Trombidiformes: Cheyletidae) and Cheyletus malaccensis Oudemans to manage Liposcelis decolor (Pearman) (Psocodea: Liposcelididae). Prey population suppression and progeny replacement efficiency of the predators were assessed under different predator–prey ratios (0:20, 1:20, 2:20, 4:20, and 10:20), temperatures (20, 24, 28, and 32 °C), and relative humidities (RH) (63, 75, and 85%) over 40 days under laboratory conditions of 0:24 (L:D) photoperiod. Suppression of L. decolor population when C. eruditus-related predator-to-prey ratios of 1:20, 2:20, 4:20, and 10:20 were used was ~61.7, 79.7, 85.1, and 87.5%, respectively, relative to the Control ratio (0:20). In the case of C. malaccensis, suppression of 70, 82.1, 92.9, and 96.5%, respectively, was achieved. Although the low 63% RH limited efficacy of these cheyletid mites, both predatory mites caused pest population suppression of ~67.1–97.2% and increased their progeny by ~96.7–844.4% fold for the predator–prey ratios of 1:20, 2:20, 4:20, and 10:20, temperatures of 20, 24, 28, and 32 °C, and RH levels of 63, 75, and 85%. The levels of psocid population suppression achieved indicate the potential of both predatory mites for psocid management.
Effect of the Sterile Insect Technique and Augmentative Parasitoid Releases in a Fruit Fly Suppression Program in Mango-Producing Areas of Southeast Mexico
Authors: Cancino Jorge , Montoya Pablo , Gálvez Fredy Orlando , Gálvez Cesar , Liedo Pablo
Published: August 2023

The Sterile Insect Technique (SIT), by means of sterile male releases of Anastrepha ludens (Loew), coupled with Augmentative Biological Control (ABC), by releasing the parasitoid Diachasmimorpha longicaudata (Ashmead), was evaluated in a commercial mango production area for one year. The obtained results were compared with mean fruit fly population values from two previous years without the combined use of both techniques. The treatments were: SIT + ABC, SIT, ABC, and Control, and each treatment was established in blocks of 5000 Ha separated by distances of 5–10 km. The evaluations were carried out through fruit sampling to assess percent parasitism and trapping of adult flies to obtain Flies per Trap per Day (FTD) values. The mean percentage of parasitism increased from 0.59% in the control treatment to 19.38% in the block with ABC. The FTD values decreased from ~0.129 and ~0.012 in the control block to 0.0021 in the block with SIT and ABC, representing a 98% suppression. The difference between the two periods in the control block was not significant. We conclude that the integration of both techniques resulted in an additive suppression of the pest population, supporting the use of both control techniques in an area-wide pest management context.
Efficient Sensing Data Collection with Diverse Age of Information in UAV-Assisted System
Authors: Pei Yanhua , Hou Fen , Zhang Guoying , Lin Bin
Published: August 2023

With the high flexibility and low cost of the deployment of UAVs, the application of UAV-assisted data collection has become widespread in the Internet of Things (IoT) systems. Meanwhile, the age of information (AoI) has been adopted as a key metric to evaluate the quality of the collected data. Most of the literature generally focuses on minimizing the age of all information. However, minimizing the overall AoI may lead to high costs and massive energy consumption. In addition, not all types of data need to be updated highly frequently. In this paper, we consider both the diversity of different tasks in terms of the data update period and the AoI of the collected sensing information. An efficient data collection method is proposed to maximize the system utility while ensuring the freshness of the collected information relative to their respective update periods. This problem is NP-hard. With the decomposition, we optimize the upload strategy of sensor nodes at each time slot, as well as the hovering positions and flight speeds of UAVs. Simulation results show that our method ensures the relative freshness of all information and reduces the time-averaged AoI by 96.5%, 44%, 90.4%, and 26% when the number of UAVs is 1 compared to the corresponding EMA, AOA, DROA, and DRL-eFresh, respectively.
Crohn’s Disease Exclusion Diet for the Treatment of Crohn’s Disease: Real-World Experience from a Tertiary Center
Authors: Fliss-Isakov Naomi , Aviv Cohen Nathaniel , Bromberg Ahuva , Elbert Gal , Anbar Ronit , Ron Yulia , Hirsch Ayal , Thurm Tamar , Maharshak Nitsan
Published: August 2023

The Crohn’s Disease (CD) exclusion diet (CDED) has been shown to induce remission in pediatric and adult patients with CD. In this retrospective cohort study, we describe our real-world experience with the CDED at the inflammatory bowel disease (IBD) unit of the Tel Aviv Medical Center between 2018–2021. CD patients with multiple clinical presentations and disease phenotypes who initiated the diet were included. Indications for treatment, medical and nutritional data were collected from dietician clinic visits and medical records. Clinical and biomarker responses were determined. The CDED was recommended to 220 CD patients. Seventy-two patients were included in the analysis for a clinically active disease (n = 48) or for remission maintenance (n = 24). Among patients with a clinically active disease, 62.5% of patients achieved clinical remission at week 6 and at week 12. A positive association between high adherence to the CDED and clinical remission at week 12 was observed (adjusted OR = 7.6, 95% CI 1.07–55.2, p = 0.043). Among patients treated for remission maintenance, remission at week 12 was maintained among 83.3% of patients. We conclude that the CDED may be a promising intervention for multiple CD presentations and indications. These findings should be further validated in larger, prospective, controlled studies.
Micronutrients in Pregnancy after Bariatric Surgery: A Narrative Review
Authors: Bretón Irene , Ballesteros-Pomar María D. , Calle-Pascual Alfonso , Alvarez-Sala Luis Antonio , Rubio-Herrera Miguel Angel
Published: August 2023

Bariatric surgery is increasingly used in women of childbearing age due to the rising prevalence of obesity and the effectiveness and availability of this treatment. Pregnancy in women with previous bariatric surgery deserves special attention. Weight loss induced by surgery reduces the risks that obesity poses to pregnancy. But on the other hand, decreased intake and malabsorption may increase the risk of malnutrition and micronutrient deficiency and negatively affect maternal and foetal health. The aim of this narrative review is to provide an updated analysis of the impact of different bariatric surgery techniques on mineral and micronutrient nutritional status during pregnancy and the possible effect on maternal–foetal health.
Lipid Profile after Pharmacologic Discontinuation and Restoration of Menstruation in Women with Endometriosis: A 12-Month Observational Prospective Study
Authors: Anastasilakis Athanasios D. , Polyzos Stergios A. , Vorkas Panagiotis A. , Gkiomisi Athina , Yavropoulou Maria P. , Rauner Martina , Nikolakopoulos Panagiotis , Papachatzopoulos Stergios , Makras Polyzois , Gerou Spyridon , Hofbauer Lorenz C. , Palermo Andrea , Tsourdi Elena
Published: August 2023

The lipid profile is affected following menstrual cessation (MC). We aimed to evaluate the effects of goserelin-induced MC and subsequent menstrual restoration (MR) on lipid metabolism. Premenopausal women with histologically verified endometriosis (n = 15) received goserelin monthly for 6 months (6mο), resulting in MC, and were followed-up for another 6 months after MR (12mο). Serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), lipoprotein a ([Lp(a)] and lipidomics were measured at baseline, 6mo and 12mo. Shotgun quantitative deep lipidomics were determined at the level of lipid class category, subclass, species, and fatty acyl chain lengths and degree of saturation. TC (p = 0.006), LDL-C (p = 0.028), HDL-C (p = 0.002), and apoA1 (p = 0.013) increased during goserelin-induced MC and remained practically unchanged during MR. TG, apoB, and Lp(a) did not change. From the deep lipidomics analysis, multivariate statistical analysis demonstrated profound alterations in lipid species with MC, whereas no statistically valid models could be fitted for the restoration period. In conclusion, GnRH-analog-induced MC alters lipid profiles at various levels, from standard blood lipid and lipoprotein profiles to several lipid species as detected by lipidomics analysis. Changes largely persist for at least 6 m after MR.
Radiomics and Artificial Intelligence for the Diagnosis and Monitoring of Alzheimer’s Disease: A Systematic Review of Studies in the Field
Authors: Bevilacqua Roberta , Barbarossa Federico , Fantechi Lorenzo , Fornarelli Daniela , Paci Enrico , Bolognini Silvia , Giammarchi Cinzia , Lattanzio Fabrizia , Paciaroni Lucia , Riccardi Giovanni Renato , Pelliccioni Giuseppe , Biscetti Leonardo , Maranesi Elvira
Published: August 2023

The use of radiomics and artificial intelligence applied for the diagnosis and monitoring of Alzheimer’s disease has developed in recent years. However, this approach is not yet completely applicable in clinical practice. The aim of this paper is to provide a systematic analysis of the studies that have included the use of radiomics from different imaging techniques and artificial intelligence for the diagnosis and monitoring of Alzheimer’s disease in order to improve the clinical outcomes and quality of life of older patients. A systematic review of the literature was conducted in February 2023, analyzing manuscripts and articles of the last 5 years from the PubMed, Scopus and Embase databases. All studies concerning discrimination among Alzheimer’s disease, Mild Cognitive Impairment and healthy older people performing radiomics analysis through machine and deep learning were included. A total of 15 papers were included. The results showed a very good performance of this approach in the differentiating Alzheimer’s disease patients—both at the dementia and pre-dementia phases of the disease—from healthy older people. In summary, radiomics and AI can be valuable tools for diagnosing and monitoring the progression of Alzheimer’s disease, potentially leading to earlier and more accurate diagnosis and treatment. However, the results reported by this review should be read with great caution, keeping in mind that imaging alone is not enough to identify dementia due to Alzheimer’s.
The Impact of Hormesis, Neuronal Stress Response, and Reproduction, upon Clinical Aging: A Narrative Review
Authors: Kyriazis Marios , Swas Lama , Orlova Tetiana
Published: August 2023

Introduction: The primary objective of researchers in the biology of aging is to gain a comprehensive understanding of the aging process while developing practical solutions that can enhance the quality of life for older individuals. This involves a continuous effort to bridge the gap between fundamental biological research and its real-world applications. Purpose: In this narrative review, we attempt to link research findings concerning the hormetic relationship between neurons and germ cells, and translate these findings into clinically relevant concepts. Methods: We conducted a literature search using PubMed, Embase, PLOS, Digital Commons Network, Google Scholar and Cochrane Library from 2000 to 2023, analyzing studies dealing with the relationship between hormetic, cognitive, and reproductive aspects of human aging. Results: The process of hormesis serves as a bridge between the biology of neuron-germ cell interactions on one hand, and the clinical relevance of these interactions on the other. Details concerning these processes are discussed here, emphasizing new research which strengthens the overall concept. Conclusions: This review presents a scientifically and clinically relevant argument, claiming that maintaining a cognitively active lifestyle may decrease age-related degeneration, and improve overall health in aging. This is a totally novel approach which reflects current developments in several relevant aspects of our biology, technology, and society.