bluuebunny/crossref_metadata_2025 huggingface dataset:
Modelling durum wheat (Triticum turgidum L. var. durum) grain protein concentration
Authors: ORLANDO F. , MANCINI M. , MOTHA R. , QU J.J. , ORLANDINI S. , DALLA MARTA A.
Published: December 2016

The goal of the present study was to improve the CERES-wheat model simulation of grain protein concentration (GPC) for winter durum wheat and to use the model as a basis for the development of a GPC Simplified Forecasting Index (SFIpro). The performances of CERES-wheat, which is one of the most widespread crop simulation models, with (i) its standard GPC routine and (ii) a novel equation developed to improve the model GPC simulation for durum wheat, were assessed through comparison with field data. Subsequently, CERES-wheat was run for a 56-year period in order to identify the most important status and forcing variables affecting GPC simulation. The number of dry days during the early growth stages and the leaf area index (LAI; green leaf area per unit ground surface area) at heading stage (LAI5) were identified as the main variables positively correlated with CERES-wheat predicted GPC, and so included in the SFIpro. At validation against observed data SFIpro was found to perform differently on the basis of observed plant LAI. In fact, SFIpro was able to forecast GPC variability for intermediate values of LAI5 ranging from 1 to 2, while it totally failed when LAI5 was outside this range (LAI5 < 1 or LAI5 > 2). The results suggest that the relationship between LAI and GPC is not linear and that the model assumptions for GPC simulation in CERES-wheat are only partially confirmed, being valid for an intermediate range of LAI.
The Historicity of the Neronian Persecution: A Response to Brent Shaw
Authors: Jones Christopher P.
Published: December 2016

Brent Shaw has recently argued that the Neronian persecution never occurred. In fact, there are no grounds for doubting Tacitus’ report of the persecution. Sources later than Tacitus (Melito, Dionysius of Alexandria) date the martyrdoms of Peter and Paul to the reign of Nero, but those reports have no bearing on the veracity of Tacitus’ account.
The Origins of Pauline Theology: Paratexts and Priscillian of Avila'sCanons on the Letters of the Apostle Paul
Authors: Lang T. J. , Crawford Matthew R.
Published: December 2016

Pauline theology is a well-established undertaking in modern New Testament studies, and yet it is almost entirely without precedent prior to the nineteenth century. This article explores the enterprise of Pauline theology by considering an important and overlooked exception to its otherwise exclusively modern provenance: Priscillian of Avila's fourth-centuryCanons on the Letters of the Apostle Paul. The key to Priscillian's dogmatic synthesis of Paul's thought was his innovative ‘versification’ of Paul's letters, which facilitated efficient citation and cross-referencing of epistolary data. This article uses Priscillian's literary creation to examine the intriguing correlation of technologies for ordering textual knowledge with the systematic abstraction of Pauline theology.
Problems for Perfectionism
Authors: BRADFORD GWEN
Published: December 2016

Perfectionism, the view that well-being is a matter of developing characteristically human capacities, has relatively few defenders in the literature, but plenty of critics. This article defends perfectionism against some recent formulations of classic objections, namely, the objection that perfectionism ignores the relevance of pleasure or preference for well-being, and a sophisticated version of the ‘wrong properties’ objection, according to which the intuitive plausibility of the perfectionist ideal is threatened by an absence of theoretical pressure to accept putative wrong properties cases. The article argues that these objections are unsuccessful, but introduce a new worry, thedeep problem: perfectionism fails to offer a satisfying foundational justification forwhydeveloping the human essence is valuable. The article responds to the deep problem, ultimately arguing that it is a puzzle put to all theories of well-being to provide a justification for their normative significance.
Risk of gestational diabetes mellitus in relation to maternal dietary calcium intake
Authors: Osorio-Yáñez Citlalli , Qiu Chunfang , Gelaye Bizu , Enquobahrie Daniel A , Williams Michelle A
Published: December 2016

Objective

The present study sought to examine the association between dietary Ca intake and risk of gestational diabetes mellitus (GDM).

Design

We assessed periconceptional (i.e. before conception and early pregnancy) Ca intake and consumption of foods rich in Ca using an FFQ among 3414 participants in a prospective cohort study. Diagnoses of GDM were abstracted from medical records. We used multivariable generalized linear regression models to derive estimates of relative risk (RR) for GDM and 95 % confidence intervals.

Setting

A prospective cohort of women in Seattle and Tacoma, WA, USA.

Subjects

Women (n 3414).

Results

A total of 169 GDM incident cases were identified in the cohort (4·96 %). Higher dietary Ca intake was inversely, although not statistically significantly, associated with GDM risk. After adjusting for confounders, the RR (95 % CI) for GDM according to successive increasing quartile of Ca intake was 1·00, 0·63 (0·41, 0·98), 0·66 (0·39, 1·11) and 0·57 (0·27, 1·21), respectively, with the lowest quartile as the reference (Ptrend=0·131). Compared with women in the first quartile for Ca intake, women in the higher three quartiles (≥795 v. <795 mg/d) had a 42 % (RR=0·58; 95 % CI 0·38, 0·90; P=0·015) lower GDM risk. GDM risk was inversely associated with low-fat dairy (Ptrend=0·032) and whole grains (Ptrend=0·019) consumption.

Conclusions

These findings suggest that higher levels of periconceptional Ca intake, particularly intake of Ca-rich low-fat dairy products and whole grains, are associated with lower GDM risk.
Supplemental Nutrition Assistance Progam benefits and emergency room visits for hypoglycaemia
Authors: Heflin Colleen , Hodges Leslie , Mueser Peter
Published: December 2016

Objective

The present study examines the connection between the timing and size of Supplemental Nutrition Assistance Program (SNAP) benefits and the occurrence of emergency room (ER) visits for hypoglycaemia, a condition that is highly sensitive to short-term changes in nutritional intake.

Design

We used administrative data from Missouri SNAP and Medicaid to identify the timing of issuance and the benefit size of SNAP and the timing of ER claims for hypoglycaemia. We estimated the probability of submitting an ER claim for hypoglycaemia as a function of the calendar week, SNAP benefit week and the size of the SNAP benefit in models that controlled for individual demographic characteristics.

Setting

Missouri SNAP caseload from January 2010 to December 2013 linked to adult Medicaid claims data for the same time period.

Subjects

ER claims submitted to Medicaid (n 6 508 061).

Results

The results indicated no evidence of a SNAP benefit cycle or monthly cycle to ER claims for hypoglycaemia. However, the analysis did find that ER claims for hypoglycaemia are related to the size of the SNAP benefit.

Conclusions

These results suggest that more generous SNAP benefits help households avoid nutritional fluctuations in the quality and quantity of food that might result in low blood sugar, thus necessitating fewer ER visits for hypoglycaemia.
‘I feel I'm Giving Something Back to Society’: Constructing the ‘Active Citizen’ and Responsibilising Foodbank Use
Authors: Garthwaite Kayleigh
Published: December 2016

Foodbanks and other forms of charitable welfare provision are fast becoming an established feature of the UK social security system. Drawing on over two years of ethnographic observation in a Trussell Trust foodbank in North East England, this paper explores the relationship between the construction of the ‘active citizen’ and lived experiences of foodbank users and volunteers. Findings show how participants’ experiences and behaviour challenges popular political and policy narratives that individuals are using foodbanks because of poor lifestyle choices. The internalisation or rejection of this narrative is then examined, contrasting the different forms of citizenship that arise. Through the significant work that goes into living on a low income, people both aligned with and challenged the ideas underpinning ‘active citizenship’.
Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
Authors: Yang Jin , Savvatis Konstantinos , Kang Jong Seok , Fan Peidong , Zhong Hongyan , Schwartz Karen , Barry Vivian , Mikels-Vigdal Amanda , Karpinski Serge , Kornyeyev Dmytro , Adamkewicz Joanne , Feng Xuhui , Zhou Qiong , Shang Ching , Kumar Praveen , Phan Dillon , Kasner Mario , López Begoña , Diez Javier , Wright Keith C. , Kovacs Roxanne L. , Chen Peng-Sheng , Quertermous Thomas , Smith Victoria , Yao Lina , Tschöpe Carsten , Chang Ching-Pin
Published: December 2016

Interstitial fibrosis plays a key role in the development and progression of heart failure. Here, we show that an enzyme that crosslinks collagen—Lysyl oxidase-like 2 (Loxl2)—is essential for interstitial fibrosis and mechanical dysfunction of pathologically stressed hearts. In mice, cardiac stress activates fibroblasts to express and secrete Loxl2 into the interstitium, triggering fibrosis, systolic and diastolic dysfunction of stressed hearts. Antibody-mediated inhibition or genetic disruption of Loxl2 greatly reduces stress-induced cardiac fibrosis and chamber dilatation, improving systolic and diastolic functions. Loxl2 stimulates cardiac fibroblasts through PI3K/AKT to produce TGF-β2, promoting fibroblast-to-myofibroblast transformation; Loxl2 also acts downstream of TGF-β2 to stimulate myofibroblast migration. In diseased human hearts, LOXL2 is upregulated in cardiac interstitium; its levels correlate with collagen crosslinking and cardiac dysfunction. LOXL2 is also elevated in the serum of heart failure (HF) patients, correlating with other HF biomarkers, suggesting a conserved LOXL2-mediated mechanism of human HF.
Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone
Authors: Huang Bin , Wang Wenhao , Li Qingchu , Wang Zhenyu , Yan Bo , Zhang Zhongmin , Wang Liang , Huang Minjun , Jia Chunhong , Lu Jiansen , Liu Sichi , Chen Hongdong , Li Mangmang , Cai Daozhang , Jiang Yu , Jin Dadi , Bai Xiaochun
Published: December 2016

Communication between osteoblasts and endothelial cells (ECs) is essential for bone turnover, but the molecular mechanisms of such communication are not well defined. Here we identify Cxcl9 as an angiostatic factor secreted by osteoblasts in the bone marrow microenvironment. We show that Cxcl9 produced by osteoblasts interacts with vascular endothelial growth factor and prevents its binding to ECs and osteoblasts, thus abrogating angiogenesis and osteogenesis both in mouse bone and in vitro. The mechanistic target of rapamycin complex 1 activates Cxcl9 expression by transcriptional upregulation of STAT1 and increases binding of STAT1 to the Cxcl9 promoter in osteoblasts. These findings reveal the essential role of osteoblast-produced Cxcl9 in angiogenesis and osteogenesis in bone, and Cxcl9 can be targeted to elevate bone angiogenesis and prevent bone loss-related diseases.
A reconstruction algorithm for compressive quantum tomography using various measurement sets
Authors: Zheng Kai , Li Kezhi , Cong Shuang
Published: December 2016

Compressed sensing (CS) has been verified that it offers a significant performance improvement for large quantum systems comparing with the conventional quantum tomography approaches, because it reduces the number of measurements from O(d2) to O(rd log(d)) in particular for quantum states that are fairly pure. Yet few algorithms have been proposed for quantum state tomography using CS specifically, let alone basis analysis for various measurement sets in quantum CS. To fill this gap, in this paper an efficient and robust state reconstruction algorithm based on compressive sensing is developed. By leveraging the fixed point equation approach to avoid the matrix inverse operation, we propose a fixed-point alternating direction method algorithm for compressive quantum state estimation that can handle both normal errors and large outliers in the optimization process. In addition, properties of five practical measurement bases (including the Pauli basis) are analyzed in terms of their coherences and reconstruction performances, which provides theoretical instructions for the selection of measurement settings in the quantum state estimation. The numerical experiments show that the proposed algorithm has much less calculating time, higher reconstruction accuracy and is more robust to outlier noises than many existing state reconstruction algorithms.
Occurrence investigation of perfluorinated compounds in surface water from East Lake (Wuhan, China) upon rapid and selective magnetic solid-phase extraction
Authors: Zhou Yusun , Tao Yun , Li Huarong , Zhou Tingting , Jing Tao , Zhou Yikai , Mei Surong
Published: December 2016

Using a novel magnetic nanocomposite as adsorbent, a convenient and effective magnetic solid-phase extraction (MSPE) procedure was established for selective separation and concentration of nine perfluorinated compounds (PFCs) in surface water sample. Then an ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) system was employed for detection of PFCs. Good linearity of the developed analytical method was in the range of 0.5–100 ng L−1 with R2 > 0.9917, and the limits of detection (LODs) ranged from 0.029 to 0.099 ng L−1. At three fortified concentrations of 0.5, 5 and 50 ng L−1, the spiked recoveries of PFCs were in the range of 90.05–106.67% with RSDs < 12.62% (n = 3). The proposed analytical method was applied for determination of PFCs in surface water from East Lake (Wuhan, China). The total concentrations of nine PFCs ranged from 30.12 to 125.35 ng L−1, with perfluorooctane sulfonate and perfluoroctanoic acid as the most prevalent PFCs, and the greatest concentrations of PFCs were observed in Niuchao lakelet. The concentrations of the PFCs (C ≥ 11) were mostly less than the limits of quantification (LOQs), attributed to the possibility that the more hydrophobic long-chain PFCs are potential to accumulate in sediment and aquatic biota.
New Immunosuppressive Sphingoid Base and Ceramide Analogues in Wild Cordyceps
Authors: Mi Jia-Ning , Han Yuwei , Xu Yingqiong , Kou Junping , Wang Jing-Rong , Jiang Zhi-Hong
Published: December 2016

A comprehensive identification of sphingoid bases and ceramides in wild Cordyceps was performed by integrating a sequential chromatographic enrichment procedure and an UHPLC-ultrahigh definition-Q-TOF-MS based sphingolipidomic approach. A total of 43 sphingoid bases and 303 ceramides were identified from wild Cordyceps, including 12 new sphingoid base analogues and 159 new ceramide analogues based on high-resolution MS and MS/MS data, isotope distribution, matching with the comprehensive personal sphingolipid database, confirmation by sphingolipid standards and chromatographic retention time rule. The immunosuppressive bioassay results demonstrated that Cordyceps sphingoid base fraction exhibits more potent immunosuppressive activity than ceramide fraction, elucidating the immunosuppressive ingredients of wild Cordyceps. This study represented the most comprehensive identification of sphingoid bases and ceramides from a natural source. The findings of this study provided an insight into therapeutic application of wild Cordyceps.
Heterogeneous shedding of influenza by human subjects and its implications for epidemiology and control
Authors: Canini Laetitia , Woolhouse Mark E. J. , Maines Taronna R. , Carrat Fabrice
Published: December 2016

Heterogeneity of infectiousness is an important feature of the spread of many infections, with implications for disease dynamics and control, but its relevance to human influenza virus is still unclear. For a transmission event to occur, an infected individual needs to release infectious particles via respiratory symptoms. Key factors to take into account are virus dynamics, particle release in relation to respiratory symptoms, the amount of virus shed and, importantly, how these vary between infected individuals. A quantitative understanding of the process of influenza transmission is relevant to designing effective mitigation measures. Here we develop an influenza infection dynamics model fitted to virological, systemic and respiratory symptoms to investigate how within-host dynamics relates to infectiousness. We show that influenza virus shedding is highly heterogeneous between subjects. From analysis of data on experimental infections, we find that a small proportion (<20%) of influenza infected individuals are responsible for the production of 95% of infectious particles. Our work supports targeting mitigation measures at most infectious subjects to efficiently reduce transmission. The effectiveness of public health interventions targeted at highly infectious individuals would depend on accurate identification of these subjects and on how quickly control measures can be applied.
Transcriptional analysis of degenerate strain Clostridium beijerinckii DG-8052 reveals a pleiotropic response to CaCO3-associated recovery of solvent production
Authors: Jiao Shengyin , Zhang Yan , Wan Caixia , Lv Jia , Du Renjia , Zhang Ruijuan , Han Bei
Published: December 2016

Degenerate Clostridium beijerinckii strain (DG-8052) can be partially recovered by supplementing CaCO3 to fermentation media. Genome resequencing of DG-8052 showed no general regulator mutated. This study focused on transcriptional analysis of DG-8052 and its response to CaCO3 treatment via microarray. The expressions of 5168 genes capturing 98.6% of C. beijerinckii NCIMB 8052 genome were examed. The results revealed that with addition of CaCO3 565 and 916 genes were significantly up-regulated, and 704 and 1044 genes significantly down-regulated at acidogenic and solventogenic phase of DG-8052, respectively. These genes are primarily responsible for glycolysis to solvent/acid production (poR, pfo), solventogensis (buk, ctf, aldh, adh, bcd) and sporulation (spo0A, sigE, sigma-70, bofA), cell motility and division (ftsA, ftsK, ftsY, ftsH, ftsE, mreB, mreC, mreD, rodA), and molecular chaperones (grpE, dnaK, dnaJ, hsp20, hsp90), etc. The functions of some altered genes in DG-8052, totalling 5.7% at acidogenisis and 8.0% at sovlentogenisis, remain unknown. The response of the degenerate strain to CaCO3 was suggested significantly pleiotropic. This study reveals the multitude of regulatory function that CaCO3 has in clostridia and provides detailed insights into degeneration mechanisms at gene regulation level. It also enables us to develop effective strategies to prevent strain degeneration in future.
A Critical Role for Toxoplasma gondii Vacuolar Protein Sorting VPS9 in Secretory Organelle Biogenesis and Host Infection
Authors: Sakura Takaya , Sindikubwabo Fabien , Oesterlin Lena K. , Bousquet Hugo , Slomianny Christian , Hakimi Mohamed-Ali , Langsley Gordon , Tomavo Stanislas
Published: December 2016

Accurate sorting of proteins to the three types of parasite-specific secretory organelles namely rhoptry, microneme and dense granule in Toxoplasma gondii is crucial for successful host cell invasion by this obligate intracellular parasite. Despite its tiny body architecture and limited trafficking machinery, T. gondii relies heavily on transport of vesicles containing proteins, lipids and important virulence-like factors that are delivered to these secretory organelles. However, our understanding on how trafficking of vesicles operates in the parasite is still limited. Here, we show that the T. gondii vacuolar protein sorting 9 (TgVps9), has guanine nucleotide exchange factor (GEF) activity towards Rab5a and is crucial for sorting of proteins destined to secretory organelles. Our results illuminate features of TgVps9 protein as a key trafficking facilitator that regulates protein maturation, secretory organelle formation and secretion, thereby ensuring a primary role in host infection by T. gondii.
Spreading to localized targets in complex networks
Authors: Sun Ye , Ma Long , Zeng An , Wang Wen-Xu
Published: December 2016

As an important type of dynamics on complex networks, spreading is widely used to model many real processes such as the epidemic contagion and information propagation. One of the most significant research questions in spreading is to rank the spreading ability of nodes in the network. To this end, substantial effort has been made and a variety of effective methods have been proposed. These methods usually define the spreading ability of a node as the number of finally infected nodes given that the spreading is initialized from the node. However, in many real cases such as advertising and news propagation, the spreading only aims to cover a specific group of nodes. Therefore, it is necessary to study the spreading ability of nodes towards localized targets in complex networks. In this paper, we propose a reversed local path algorithm for this problem. Simulation results show that our method outperforms the existing methods in identifying the influential nodes with respect to these localized targets. Moreover, the influential spreaders identified by our method can effectively avoid infecting the non-target nodes in the spreading process.
Subtype-specific structural constraints in the evolution of influenza A virus hemagglutinin genes
Authors: Gultyaev Alexander P. , Spronken Monique I. , Richard Mathilde , Schrauwen Eefje J. A. , Olsthoorn René C. L. , Fouchier Ron A. M.
Published: December 2016

The influenza A virus genome consists of eight RNA segments. RNA structures within these segments and complementary (cRNA) and protein-coding mRNAs may play a role in virus replication. Here, conserved putative secondary structures that impose significant evolutionary constraints on the gene segment encoding the surface glycoprotein hemagglutinin (HA) were investigated using available sequence data on tens of thousands of virus strains. Structural constraints were identified by analysis of covariations of nucleotides suggested to be paired by structure prediction algorithms. The significance of covariations was estimated by mutual information calculations and tracing multiple covariation events during virus evolution. Covariation patterns demonstrated that structured domains in HA RNAs were mostly subtype-specific, whereas some structures were conserved in several subtypes. The influence of RNA folding on virus replication was studied by plaque assays of mutant viruses with disrupted structures. The results suggest that over the whole length of the HA segment there are local structured domains which contribute to the virus fitness but individually are not essential for the virus. Existence of subtype-specific structured regions in the segments of the influenza A virus genome is apparently an important factor in virus evolution and reassortment of its genes.
Gait patterns associated with thyroid function: The Rotterdam Study
Authors: Bano Arjola , Chaker Layal , Darweesh Sirwan K. L. , Korevaar Tim I. M. , Mattace-Raso Francesco U. S. , Dehghan Abbas , Franco Oscar H. , van der Geest Jos N. , Ikram M. Arfan , Peeters Robin P.
Published: December 2016

Gait is an important health indicator and poor gait is strongly associated with disability and risk of falls. Thyroid dysfunction is suggested as a potential determinant of gait deterioration, but this has not been explored in a population-based study. We therefore investigated the association of thyroid function with gait patterns in 2645 participants from the Rotterdam Study with data available on TSH (thyroid-stimulating hormone), FT4 (free thyroxine) and gait, without known thyroid disease or dementia. The primary outcome was Global gait (standardized Z-score), while secondary outcomes included gait domains (Rhythm, Variability, Phases, Pace, Base of support, Tandem, Turning) and velocity. Gait was assessed by electronic walkway. Multivariable regression models revealed an inverted U-shaped association of TSH (p < 0.001), but no association of FT4 concentrations with Global gait (p = 0.2). TSH levels were positively associated with Base of support (p = 0.01) and followed an inverted U-shaped curve with Tandem (p = 0.002) and velocity (p = 0.02). Clinical and subclinical hypothyroidism were associated with worse Global gait than euthyroidism (β = −0.61; CI = −1.03, −0.18; p = 0.004 and β = −0.13; CI = −0.26, −0.00; p = 0.04, respectively). In euthyroid participants, higher thyroid function was associated with worse gait patterns. In conclusion, both low and high thyroid function are associated with alterations in Global gait, Tandem, Base of support and velocity.
Urinary KIM-1: a novel biomarker for evaluation of occupational exposure to lead
Authors: Zhou Rong , Xu Yahong , Shen Jie , Han Lin , Chen Xi , Feng Xuefang , Kuang Xingya
Published: December 2016

Chronic occult lead poisoning often develops ensuing occupational lead exposure. Early diagnosis of lead poisoning is critical for timely discontinuation of lead exposure and for prognosis. This study explored the value of urinary kidney injury molecule-1 (KIM-1) in diagnosing renal injury induced by lead at an early stage. We retrospectively analyzed 92 workers exposed to occupational lead and demonstrated a better correlation ship between blood lead levels and urine excretion of KIM-1 than other traditional renal injury biomarkers following creatinine adjustment. Receiver operating characteristic curve analysis of the ability of diverse biomarkers for predicting kidney injury in lead-exposed workers demonstrated that the order of predicting accuracy of the studied biomarkers is as follows: urinary KIM-1-to-creatinine ratio > urinary N-acetyl-β-(D)-glucosaminidase-to-creatinine ratio > urinary β2-microglobulin-to-creatinine ratio > urinary α1-microglobulin-to-creatinine ratio, with the Youden index being 16.59 ng/g, 14.01 U/g, 0.15 mg/g, and 4.63 mg/g, respectively. Collectively, our findings suggest that short-period occupational lead exposure may cause injury of renal tubules. Urinary excretion of KIM-1 correlates with blood lead levels better than other traditional renal injury biomarkers, including N-acetyl-β-(D)-glucosaminidase, α1-microglobulin, and β2-microglobulin. Longitudinal surveillance of urinary KIM-1 may aid for early diagnosis of renal tubular injury in workers with occupational lead exposure.
Detecting the existence of gene flow between Spanish and North African goats through a coalescent approach
Authors: Martínez Amparo , Manunza Arianna , Delgado Juan Vicente , Landi Vincenzo , Adebambo Ayotunde , Ismaila Muritala , Capote Juan , El Ouni Mabrouk , Elbeltagy Ahmed , Abushady Asmaa M. , Galal Salah , Ferrando Ainhoa , Gómez Mariano , Pons Agueda , Badaoui Bouabid , Jordana Jordi , Vidal Oriol , Amills Marcel
Published: December 2016

Human-driven migrations are one of the main processes shaping the genetic diversity and population structure of domestic species. However, their magnitude and direction have been rarely analysed in a statistical framework. We aimed to estimate the impact of migration on the population structure of Spanish and African goats. To achieve this goal, we analysed a dataset of 1,472 individuals typed with 23 microsatellites. Population structure of African and Spanish goats was moderate (mean FST = 0.07), with the exception of the Canarian and South African breeds that displayed a significant differentiation when compared to goats from North Africa and Nigeria. Measurement of gene flow with Migrate-n and IMa coalescent genealogy samplers supported the existence of a bidirectional gene flow between African and Spanish goats. Moreover, IMa estimates of the effective number of migrants were remarkably lower than those calculated with Migrate-n and classical approaches. Such discrepancies suggest that recent divergence, rather than extensive gene flow, is the main cause of the weak population structure observed in caprine breeds.
Meteorite Impact-Induced Rapid NH3 Production on Early Earth: Ab Initio Molecular Dynamics Simulation
Authors: Shimamura Kohei , Shimojo Fuyuki , Nakano Aiichiro , Tanaka Shigenori
Published: December 2016

NH3 is an essential molecule as a nitrogen source for prebiotic amino acid syntheses such as the Strecker reaction. Previous shock experiments demonstrated that meteorite impacts on ancient oceans would have provided a considerable amount of NH3 from atmospheric N2 and oceanic H2O through reduction by meteoritic iron. However, specific production mechanisms remain unclear, and impact velocities employed in the experiments were substantially lower than typical impact velocities of meteorites on the early Earth. Here, to investigate the issues from the atomistic viewpoint, we performed multi-scale shock technique-based ab initio molecular dynamics simulations. The results revealed a rapid production of NH3 within several picoseconds after the shock, indicating that shocks with greater impact velocities would provide further increase in the yield of NH3. Meanwhile, the picosecond-order production makes one expect that the important nitrogen source precursors of amino acids were obtained immediately after the impact. It was also observed that the reduction of N2 proceeded according to an associative mechanism, rather than a dissociative mechanism as in the Haber-Bosch process.
Magnetic Phases of Sputter Deposited Thin-Film Erbium
Authors: Witt J. D. S. , Cooper J. F. K. , Satchell N. , Kinane C. J. , Curran P. J. , Bending S. J. , Langridge S. , Heyderman L. J. , Burnell G.
Published: December 2016

We present a detailed structural and magnetic characterization of sputter deposited thin film erbium, determined by x-ray diffraction, transport measurements, magnetometry and neutron diffraction. This provides information on the onset and change of the magnetic state as a function of temperature and applied magnetic field. Many of the features of bulk material are reproduced. Also of interest is the identification of a conical magnetic state which repeats with a wavevector parallel to the c axis τc = 4/17 in units of the reciprocal lattice parameter c*, which is a state not observed in any other thin film or bulk measurements. The data from the various techniques are combined to construct magnetic field, temperature (H, T)–phase diagrams for the 200 nm-thick Er sample that serves as a foundation for future exploitation of this complex magnetic thin film system.
Comparative Proteomics Reveals that Phosphorylation of β Carbonic Anhydrase 1 Might be Important for Adaptation to Drought Stress in Brassica napus
Authors: Wang Limin , Jin Xiang , Li Qingbin , Wang Xuchu , Li Zaiyun , Wu Xiaoming
Published: December 2016

Little is known about the mechanism of drought tolerance in rapeseed (Brassica napus L.). In this study, different morphological and physiological responses to drought stress were studied in three rapeseed cultivars. For the cultivar 2AF009 with high drought tolerance, comparative proteomic analyses were conducted to determine the molecular mechanism behind. Approximately 138 differentially abundant proteins (DAPs) and 1232 phosphoproteins containing 4469 phosphopeptides were identified. Furthermore, 337 phosphoproteins containing 547 phosphorylation sites demonstrated significant changes. These drought-responsive DAPs and phosphoproteins were mainly involved in signal transduction, photosynthesis, and glutathione-ascorbate metabolism. Notably, 9 DAPs were also identified as drought-responsive phosphoproteins, especially beta carbonic anhydrase 1 (βCA1), which was represented by eight distinct protein spots with different abundant levels during drought stress. Tyr207 phosphorylated site of βCA1 was down-regulated at the phosphorylation level during drought stress, which was also located in the substrate-binding active region of three-dimensional (3D) structure. Moreover, drought stress inhibited CA activity. We concluded that Tyr207 was the most likely phosphorylation target affecting the enzyme activity, and phosphorylation of βCA1 might be important for the response to drought stress in rapeseed. The study provided a new clue for the drought tolerance mechanism in B.napus.
Microwave-assisted cross-linking of milk proteins induced by microbial transglutaminase
Authors: Chen Chun-Chi , Hsieh Jung-Feng
Published: December 2016

We investigated the combined effects of microbial transglutaminase (MTGase, 7.0 units/mL) and microwave irradiation (MI) on the polymerization of milk proteins at 30 °C for 3 h. The addition of MTGase caused the milk proteins to become polymerized, which resulted in the formation of components with a higher molecular-weight (>130 kDa). SDS-PAGE analysis revealed reductions in the protein content of β-lactoglobulin (β-LG), αS-casein (αS-CN), κ-casein (κ-CN) and β-casein (β-CN) to 50.4 ± 2.9, 33.5 ± 3.0, 4.2 ± 0.5 and 1.2 ± 0.1%, respectively. The use of MTGase in conjunction MI with led to a 3-fold increase in the rate of milk protein polymerization, compared to a sample that contained MTGase but did not undergo MI. Results of two-dimensional gel electrophoresis (2-DE) indicated that κ-CN, β-CN, a fraction of serum albumin (SA), β-LG, α-lactalbumin (α-LA), αs1-casein (αs1-CN), and αs2-casein (αs2-CN) were polymerized in the milk, following incubation with MTGase and MI at 30 °C for 1 h. Based on this result, the combined use of MTGase and MI appears to be a better way to polymerize milk proteins.
Molecular mechanism: the human dopamine transporter histidine 547 regulates basal and HIV-1 Tat protein-inhibited dopamine transport
Authors: Quizon Pamela M. , Sun Wei-Lun , Yuan Yaxia , Midde Narasimha M. , Zhan Chang-Guo , Zhu Jun
Published: December 2016

Abnormal dopaminergic transmission has been implicated as a risk determinant of HIV-1-associated neurocognitive disorders. HIV-1 Tat protein increases synaptic dopamine (DA) levels by directly inhibiting DA transporter (DAT) activity, ultimately leading to dopaminergic neuron damage. Through integrated computational modeling prediction and experimental validation, we identified that histidine547 on human DAT (hDAT) is critical for regulation of basal DA uptake and Tat-induced inhibition of DA transport. Compared to wild type hDAT (WT hDAT), mutation of histidine547 (H547A) displayed a 196% increase in DA uptake. Other substitutions of histidine547 showed that DA uptake was not altered in H547R but decreased by 99% in H547P and 60% in H547D, respectively. These mutants did not alter DAT surface expression or surface DAT binding sites. H547 mutants attenuated Tat-induced inhibition of DA transport observed in WT hDAT. H547A displays a differential sensitivity to PMA- or BIM-induced activation or inhibition of DAT function relative to WT hDAT, indicating a change in basal PKC activity in H547A. These findings demonstrate that histidine547 on hDAT plays a crucial role in stabilizing basal DA transport and Tat-DAT interaction. This study provides mechanistic insights into identifying targets on DAT for Tat binding and improving DAT-mediated dysfunction of DA transmission.
Reduced activation in isometric muscle action after lengthening contractions is not accompanied by reduced performance fatigability
Authors: Seiberl W. , Hahn D. , Paternoster F. K.
Published: December 2016

After active lengthening contractions, a given amount of force can be maintained with less muscle activation compared to pure isometric contractions at the same muscle length and intensity. This increase in neuromuscular efficiency is associated with mechanisms of stretch-induced residual force enhancement. We hypothesized that stretch-related increase in neuromuscular efficiency reduces fatigability of a muscle during submaximal contractions. 13 subjects performed 60 s isometric knee extensions at 60% of maximum voluntary contraction (MVC) with and without prior stretch (60°/s, 20°). Each 60 s trial was preceded and followed by neuromuscular tests consisting of MVCs, voluntary activation (VA) and resting twitches (RT), and there was 4 h rest between sets. We found a significant (p = 0.036) 10% reduction of quadriceps net-EMG after lengthening compared to pure isometric trials. However, increase in neuromuscular efficiency did not influence the development of fatigue. Albeit we found severe reduction of MVC (30%), RT (30%) and VA (5%) after fatiguing trials, there were no differences between conditions with and without lengthening. As the number of subjects showing no activation reduction increased with increasing contraction time, intensity may have been too strenuous in both types of contractions, such that a distinction between different states of fatigue was not possible anymore.
Multivalent pIX phage display selects for distinct and improved antibody properties
Authors: Høydahl Lene S. , Nilssen Nicolay R. , Gunnarsen Kristin S. , Pré M. Fleur du , Iversen Rasmus , Roos Norbert , Chen Xi , Michaelsen Terje E. , Sollid Ludvig M. , Sandlie Inger , Løset Geir Å.
Published: December 2016

Phage display screening readily allows for the identification of a multitude of antibody specificities, but to identify optimal lead candidates remains a challenge. Here, we direct the antibody-capsid fusion away from the signal sequence-dependent secretory SEC pathway inE. coliby utilizing the intrinsic signal sequence-independent property of pIX to obtain virion integration. This approach was combined with the use of an engineered helper phage known to improve antibody pIX display and retrieval. By direct comparison with pIII display, we demonstrate that antibody display using this pIX system translates into substantially improved retrieval of desired specificities with favorable biophysical properties inde novoselection. We show that the effect was due to lessE. colihost toxicity during phage propagation conferred by the lack of a signal sequence. This pIX combinatorial display platform provides a generic alternative route for obtaining good binders with high stability and may thus find broad applicability.
NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis
Authors: Itani Shigehiro , Watanabe Toshio , Nadatani Yuji , Sugimura Naoki , Shimada Sunao , Takeda Shogo , Otani Koji , Hosomi Shuhei , Nagami Yasuaki , Tanaka Fumio , Kamata Noriko , Yamagami Hirokazu , Tanigawa Tetsuya , Shiba Masatsugu , Tominaga Kazunari , Fujiwara Yasuhiro , Arakawa Tetsuo
Published: December 2016

The inflammasomes induce maturation of pro-interleukin-1β (IL-1β) and pro-IL-18. We investigated roles of the NLRP3 inflammasome in the pathogenesis of ulcerative colitis (UC). After induction of oxazolone-induced colitis, a mouse UC model, colonic tissues were assayed for inflammatory mediators. Histological studies were performed on inflamed colonic tissue from mice and UC patients. Histological severity of murine colitis peaked on day 1, accompanied by an increase in the expression of Th2 cytokines including IL-4 and IL-13. Oxazolone treatment stimulated maturation of pro-caspase-1 and pro-IL-1β, while it reduced IL-18 expression. Either exogenous IL-1β or IL-18 ameliorated the colitis with or without reduction in Th2 cytokine expression, respectively. Induction of colitis decreased MUC2 expression, which was reversed by administration of IL-18, but not IL-1β. Compared to wild-type mice, NLRP3−/− mice exhibited higher sensitivity to oxazolone treatment with enhancement of Th2 cytokine expression and reduction of mature IL-1β and IL-18 production; this phenotype was rescued by exogenous IL-1β or IL-18. Immunofluorescent studies revealed positive correlation of NLRP3 expression with disease severity in UC patients, and localization of the inflammasome-associated molecules in macrophages. The NLRP3 inflammasome-derived IL-1β and IL-18 may play a protective role against UC through different mechanisms.
The Spatial Relationship of Malignant and Benign Breast Lesions with Respect to the Fat-Gland Interface on Magnetic Resonance Imaging
Authors: Kim Won Hwa , Li MuLan , Han Wonshik , Ryu Han Suk , Moon Woo Kyung
Published: December 2016

The fat-gland interface in the breast is noteworthy in that major vessels and lymphatic channels supplying the breast are located there; however, the relationship between breast lesion formation and the fat-gland interface is poorly understood. Here we evaluate the location of malignant and benign breast lesions with respect to the fat-gland interface in 881 women 50 years of age and younger, utilizing MR imaging. We find that most breast lesions are located in or near the interface in qualitative (89.7%) and quantitative (90.0%, 1 cm within the interface) analyses. This propensity for the fat-gland interface is not accounted for by breast anatomy, whereby 12.3% and 55.7% of breast volume is within 2 mm and 1 cm of the interface, respectively. Malignant lesions were located in or near the interface in significantly higher proportions than benign lesions in qualitative (94.3% vs. 67.3%, P < 0.001) and quantitative (49.7% vs. 34.5%, P < 0.001, 2 mm within the interface) analyses. This phenomenon may reflect a biological importance of the fat-gland interface in breast cancer development and progression.
Exceptionally omnidirectional broadband light harvesting scheme for multi-junction concentrator solar cells achieved via ZnO nanoneedles
Authors: Yeh Li-Ko , Tian Wei-Cheng , Lai Kun-Yu , He Jr-Hau
Published: December 2016

GaInP/GaAs/Ge triple-junction concentrator solar cells with significant efficiency enhancement were demonstrated with antireflective ZnO nanoneedles. The novel nanostructure was attained with a Zn(NO3)2-based solution containing vitamin C. Under one sun AM 1.5G solar spectrum, conversion efficiency of the triple-junction device was improved by 23.7% via broadband improvement in short-circuit currents of 3 sub-cells after the coverage by the nanoneedles with a graded refractive index profile. The efficiency enhancement further went up to 45.8% at 100 suns. The performance boost through the nanoneedles also became increasingly pronounced in the conditions of high incident angles and the cloudy weather, e.g. 220.0% of efficiency enhancement was observed at the incident angle of 60°. These results were attributed to the exceptional broadband omnidirectionality of the antireflective nanoneedles.
Variation in the TAS2R31 bitter taste receptor gene relates to liking for the nonnutritive sweetener Acesulfame-K among children and adults
Authors: Bobowski Nuala , Reed Danielle R. , Mennella Julie A.
Published: December 2016

The nonnutritive sweetener (NNS) acesulfame potassium (Ace-K) elicits a bitter off-taste that varies among adults due to polymorphisms in a bitter taste receptor gene. Whether polymorphisms affect liking for Ace-K by children, who live in different sensory worlds, is unknown. We examined hedonic response to Ace-K among children compared to adults, and whether response was related to common variants of the TAS2R31 bitter taste receptor gene and to NNS intake. Children (N = 48) and their mothers (N = 34) rated liking of Ace-K, and mothers reported whether they or their children ever consume NNSs via questionnaire. Participants were genotyped for TAS2R31 variant sites associated with adult perception of Ace-K (R35W, L162M, A227V, and V240I). Regardless of age, more participants with 1 or no copies than with 2 copies of the TAS2R31 WMVI haplotype liked Ace-K (p = 0.01). NNS-sweetened products were consumed by 50% and 15% of mothers and children, respectively, with no association between intake and TAS2R31. The TAS2R31 WMVI haplotype was partly responsible for children’s hedonic response to Ace-K, highlighting a potential role for inborn differences in vulnerability to overconsumption of Ace-K-containing products. Currently available methods to measure NNS intake yield crude estimates at best, suggesting self-reports are not reflective of actual intake.
Synthesis of Mesoporous α-Fe2O3 Nanoparticles by Non-ionic Soft Template and Their Applications to Heavy Oil Upgrading
Authors: Park Chulwoo , Jung Jinhwan , Lee Chul Wee , Cho Joungmo
Published: December 2016

This paper reports the synthetic route of 3-D network shape α-Fe2O3 from aqueous solutions of iron precursor using a non-ionic polymeric soft-template, Pluronic P123. During the synthesis of α-Fe2O3, particle sizes, crystal phases and morphologies were significantly influenced by pH, concentrations of precursor and template. The unique shape of worm-like hematite was obtained only when a starting solution was prepared by a weakly basic pH condition and a very specific composition of constituents. The synthesized nanocrystal at this condition had a narrow pore size distribution and high surface area compared to the bulk α-Fe2O3 or the one synthesized from lower pH conditions. The hydrocracking performance was tested over the synthesized iron oxide catalysts with different morphologies. The worm-like shape of iron oxide showed a superior performance, including overall yield of liquid fuel product and coke formation, over the hydrocracking of heavy petroleum oil.
Neonatal repetitive pain in rats leads to impaired spatial learning and dysregulated hypothalamic-pituitary-adrenal axis function in later life
Authors: Chen Mengying , Xia Dongqing , Min Cuiting , Zhao Xiaoke , Chen Yinhua , Liu Li , Li Xiaonan
Published: December 2016

Preterm birth is a major health issue. As part of their life-saving care, most preterm infants require hospitalization and are inevitably exposed to repetitive skin-breaking procedures. The long-term effects of neonatal repetitive pain on cognitive and emotional behaviors involving hypothalamic-pituitary-adrenal (HPA) axis function in young and adult rats are unknown. From P8 to P85, mechanical hypersensitivity of the bilateral hindpaws was observed in the Needle group (P < 0.001). Compared with the Tactile group, the Needle group took longer to find the platform on P30 than on P29 (P = 0.03), with a decreased number of original platform site crossings during the probe trial of the Morris water maze test (P = 0.026). Moreover, the Needle group spent more time and took longer distances in the central area than the Tactile group in the Open-field test, both in prepubertal and adult rats (P < 0.05). The HPA axis function in the Needle group differed from the Tactile group (P < 0.05), with decreased stress responsiveness in prepuberty and puberty (P < 0.05) and increased stress responsiveness in adulthood (P < 0.05). This study indicates that repetitive pain that occurs during a critical period may cause severe consequences, with behavioral and neuroendocrine disturbances developing through prepuberty to adult life.
Restoration of foveal photoreceptors after intravitreal ranibizumab injections for diabetic macular edema
Authors: Mori Yuki , Suzuma Kiyoshi , Uji Akihito , Ishihara Kenji , Yoshitake Shin , Fujimoto Masahiro , Dodo Yoko , Yoshitake Tatsuya , Miwa Yuko , Murakami Tomoaki
Published: December 2016

Anti-vascular endothelial growth factor drugs are the first-line treatment for diabetic macular edema (DME), although the mechanism of the visual acuity (VA) improvement remains largely unknown. The association between photoreceptor damage and visual impairment encouraged us to retrospectively investigate the changes in the foveal photoreceptors in the external limiting membrane (ELM) and ellipsoid zone (EZ) on spectral-domain optical coherence tomography (SD-OCT) images in 62 eyes with DME treated with intravitreal ranibizumab (IVR) injections. The transverse lengths of the disrupted EZ and ELM were shortened significantly (P < 0.001 and P = 0.044, respectively) at 12 months. The qualitative investigation also showed restoration of the EZ and ELM lines on SD-OCT images. The EZ at 12 months lengthened in 34 of 38 eyes with discontinuous EZ and was preserved in 16 of 21 eyes with complete EZ at baseline. VA improvement was positively correlated with shortening of the disrupted EZ at 12 months (ρ = 0.463, P <0.001), whereas the decrease in central subfield thickness was associated with neither VA improvement nor changes in EZ status (ρ = 0.215, P = 0.093 and (ρ = 0.209, P = 0.103, respectively). These data suggested that photoreceptor restoration contributes to VA improvement after pro re nata treatment with IVR injections for DME independent of resolved retinal thickening.