bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan Aroma Therapy Bunga (rose centifolia) dan Pijat Bayi Terhadap Durasi Tidur Bayi Usia 3-12 Bulan di RSUD Poso
Authors: Cindy Niluh , Suharto Dewi Nurviana
Published: August 2023

Introduction: The impact of sleep disturbances in infants is that the baby becomes often awake and fussy. The importance of sleep time in infants, then the duration of sleep in infants must be completely fulfilled so as not to adversely affect their development. One of the nursing interventions to treat sleep disorders in infants is massage and rose aroma therapy. The purpose of this study was to describe the application of nursing care with massage interventions and rose aroma therapy to increase infant sleep duration. Method : Descriptive research methods with a case study approach. Results : the patient's mother said her child had a cough with phlegm, fever for 3 days, the patient was fussy and restless, the patient slept only ± 8 hours a day, the patient often woke up at night due to coughing, the patient was difficult to sleep well, the patient's face looked pale, often seen yawning, temperature: 38.9 ° C RR 46x / min, pulse 120x / min, there are additional ronchi breath sounds, nursing diagnosis of airway clearance, hyperthermia, sleep pattern disorders. Nursing interventions for airway management, warm compresses, massage therapy and aromatherapy. Implementation of chest physiotherapy measures as well as giving 1ml combiven nebulizer + 2ml Nacl and suctioning. Giving tepid water sponge, doing baby massage and giving rose aroma therapy. The results of the evaluation of the patient's sleep increased from 8 hours to 12 hours a day, the patient was calm, the patient woke up at night only asking for milk and after waking up the patient could fall asleep again, coughing with phlegm was reduced, vesicular breath sounds, temperature: 37°C, RR: 38x/min. Conclusion: giving baby massage and rose aroma therapy can increase the duration of infant sleep. nurses can provide baby massage combined with rose aroma therapy to increase the duration of infant sleep.
Penerapan Perawatan Kaki Dan Kuku Terhadap Peningkatan Sirkulasi Perifer Pada Penderita Critical Limb Ischemia (CLI)
Authors: Fantri Nurfantri , Saranani Muhaimin , Iriani Iluh
Published: August 2023

Background . Critical limb ischemia (CLI) is the most severe stages disease vessels blood chronic , oriented CLI management on effort to anticipate  doing amputation by pushing  a severe infection happens on wound and healing the wound easier.  One wrong possible intervention  done to increase peripheral circulation and prevent the occurance of amputation consequence on severe infection is circulation maintenance. Purpose Describe feet application and nail care _ enhancement of peripheral circulation to fill a need safety of patients with Critical Limb Ischemia (CLI) . Method :  This is a descriptive study uses a study case approach with a Nursing process approach . Subject of the study was one  participant . Results  the client felt painful on the feet , it started by feeling numb and cold on her left toes, then purple spots and several days later the spots were changed to be black and widespread to her calf . cold acral, nursing diagnosis enforced impaired peripheral tissue perfusion. Nursing Intervention: feet and nail care, Evaluation: decreased pain scale, increased muscle strength, changed in acral temperature and no changed in capillary refill. Conclusion : Giving circulation maintenance on client Mrs. S with CLI (Critical Limb Ischemia) for 5 days management was effective because on the fifth day client said painful on her left lower extremity decreased so that she could move , and client said she felt comfortable and relax after washing and cleaning her feet using warm water , soap , body lotion and cuting her toenails.

Keywords : CLI, foot care , peripheral circulation, pain
Pengaruh Teknik Relaksasi Napas Dalam Terhadap Nyeri Post Sectio Caesarea di RSUD Rupit
Authors: Herdiani Tria Nopi , Fitriani Desi
Published: August 2023

Introduction :  Labor by Sectio Caesarea, the mother will experience mild to severe pain. Pain will cause feelings of discomfort, if this is not addressed immediately it will interfere with the healing process and in an effort to reduce pain in caesarean section patients, one of them is using deep breathing relaxation techniques that can reduce the pain scale in post Sectio Caesarea.The purpose of the study was to determine the effect of deep breathing relaxation techniques on post sectio caesarea pain in Rupit Hospital. Method : This study uses a pre-experimental research design with a one group pretest posttest design. The population of this study were all postoperative sectio caesarea patients who received treatment in the Aster Room of Rupit Hospital. Sampling using purposive sampling in this study a sample of 20 postoperative sectio caesarea patients. The data in this study are primary data using a pain scale sheet and secondary data using patient medical records. Hypothesis prereqisite test using Shapiro Wilk test. The hypothesis test used is the Paired Sample T-Test. Result : from univariate data obtained an image of post sectio caesarea the average pain before being given deep breathing relaxation techniques was 5.30 and after being given deep breathing relaxation techniques 2.85. From the bivariate data on the normality test of the pain scale before and after deep breathing relaxation (p > 0.05), the data were normally distributed. And the Paired Sample T-Test hypothesis test found p-value < 0.000. Conclusion : there is an effect of deep breathing relaxation techniques on post sectio caesarea pain in Rupit Hospital.
Impact of Early Marriage on Women
Authors: Hadina Hadina , Longgupa Lisda Widianti , Rahmawati Rahmawati , Radjulaeni Zakiah
Published: August 2023

Child marriage is a marriage performed by youth under the age of 19. There were 27 cases of child marriage in 2019 in Sigi Biromaru District and in 2020 there has been an increase of 46 cases. Purpose This study aims to examine the impact caused by child marriage in Kalukubula Village Method This type of research uses a qualitative phenomenological approach. The population in this study were all women who had child marriages in the village of Kalukubula. Informant collection techniques in this study using Snowball Sampling. Data were collected by in-depth interview techniques using interview guidelines. The informants in this study were 10 informants, 5 main informants, namely women who had child marriages, 4 parents as key informants, and the head of KUA as additional informants. Results obtained are that the impact of child marriage is Psychological Impact, stress due to unpreparedness in managing the household and in taking care of children, Biological Impact, is an impact that occurs on health, economic impact, due to difficulties in finding work due to low educational status, Impact Education is one of the impacts of child marriage due to dropping out of school so that when married it is difficult to find jobs. Conclusion Child marriage in Kalukubula Village has psychological, biological, economic and educational impacts. It is recommended that local government parents prevent child marriage from occurring.
Relationship of Diet and Menstrual Cycle with Anemia in Young Women
Authors: Hadriani Hadriani , Entoh Christina , Radjulaeni Zakiah , Astuti Meike Dwi
Published: August 2023

Background: Anemia is a problem that occurs in young women which results in decreased productivity, mental development, and intelligence. Based on the Indonesian Basic Health Research, the prevalence of anemia in young women has increased from 2013 (22.7%) to 2018 (32%). Objective: to determine the relationship between diet and menstrual cycle with anemia in young women at State Senior High School (SMAN) 7 Palu City. Methods: observational analytic, using a cross-sectional approach. The number of samples is 45 young women with random sampling technique. Data were analyzed by univariate test and bivariate test. Bivariate test analysis using the Chi-Square test. Results: Analysis of the relationship between eating patterns and anemia in female adolescents OR 3.238 (p=0.058). Analysis of the relationship between the menstrual cycle and anemia in young women OR 5.950 (= 0.007). Conclusion: There is an effect of health education with leaflet media on women's knowledge about cervical cancer screening. It is expected that health workers will regularly provide health education
WHY ARE EPOXY-COATED STEEL REBARS PROHIBITED
IN BRIDGE STRUCTURES IN SOME REGIONS?
Authors: Gulyayev Valery , Mozgovyy Volodymyr , Shlyun Natalia
Published: August 2023

One of the common ways in many countries to protect steel reinforcement from corrosion in reinforced concrete structures is to cover its surface with a thin layer of epoxy resin. However, in practice, in many cases, the anti-corrosion effect of epoxy coating was not significant, and the durability of reinforced con-crete bridge structures was lower than those where steel reinforcement was not coated with epoxy resin. Ex-perts believe that one of the factors that affects the corrosion resistance of steel reinforcement with epoxy coat-ing in reinforced concrete is the presence of mechanical damage in the epoxy coating of the reinforcing rod, through which moisture can penetrate. Therefore, in some countries, instructions have been developed for the prevention of such damage at the stages of applying an epoxy coating to the armature, its storage, transporta-tion, and construction and assembly work in the manufacture of reinforced concrete structures. This article discusses the thermomechanical mechanism of violation of the internal structural integrity of concrete rein-forced with steel rods with an epoxy coating, which is caused by a high value of its coefficient of linear thermal expansion and the epoxy coating itself. Using the methods of the theory of thermoelasticity, a mathematical model of this phenomenon was developed, a system of solving differential equations was formed, and its solu-tion was constructed. It was established that even with relatively minor temperature changes in the concrete environment in the area of its contact with the reinforcement, cracks and damages occur along the entire length of the steel reinforcement. All this contributes to further destructive processes and reducing the durabil-ity of reinforced concrete bridge structures.
Immunity towards human respiratory syncytial virus
Authors: Correa Dahiana , Giraldo Diana M. , Gallego Salomon , Taborda Natalia A. , Hernandez Juan C.
Published: August 2023

Respiratory infections remain a significant cause of morbidity and mortality, becoming a serious public health issue worldwide. The human respiratory syncytial virus (hRSV) is still one of the most relevant pathogenic agents involved in respiratory infections in children, the leading cause of bronchiolitis worldwide. In most cases, hRSV infection is not complicated; however, limited treatment and vaccine options increase the morbidity rates associated with bronchiolitis. The innate immune response governs the severity of the disease and controls the viral infection outcome. Current knowledge about the mechanisms involved in viral PAMPs (pathogen-associated molecular pattern molecules) recognition, genetic characteristics of the inflammatory response, and understanding of antiviral response is crucial for vaccine development and biomarker tools to predict complications and guide therapeutic management. Here, we review key concepts related to pathogenesis and immune response against hRSV, highlighting aspects that could be considered in vaccine development.
Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility
Authors: Bilekova Sara , Garcia-Colomer Balma , Cebrian-Serrano Alberto , Schirge Silvia , Krey Karsten , Sterr Michael , Kurth Thomas , Hauck Stefanie M. , Lickert Heiko
Published: August 2023

Spermatogenesis is a crucial biological process that enables the production of functional sperm, allowing for successful reproduction. Proper germ cell differentiation and maturation require tight regulation of hormonal signals, cellular signaling pathways, and cell biological processes. The acrosome is a lysosome-related organelle at the anterior of the sperm head that contains enzymes and receptors essential for egg-sperm recognition and fusion. Even though several factors crucial for acrosome biogenesis have been discovered, the precise molecular mechanism of pro-acrosomal vesicle formation and fusion is not yet known. In this study, we investigated the role of the insulin inhibitory receptor (inceptor) in acrosome formation. Inceptor is a single-pass transmembrane protein with similarities to mannose-6-phosphate receptors (M6PR). Inceptor knockout male mice are infertile due to malformations in the acrosome and defects in the nuclear shape of spermatozoa. We show that inceptor is expressed in early spermatids and mainly localizes to vesicles between the Golgi apparatus and acrosome. Here we show that inceptor is an essential factor in the intracellular transport of trans-Golgi network-derived vesicles which deliver acrosomal cargo in maturing spermatids. The absence of inceptor results in vesicle-fusion defects, acrosomal malformation, and male infertility. These findings support our hypothesis of inceptor as a universal lysosomal or lysosome-related organelle sorting receptor expressed in several secretory tissues.
RETRACTED: Spectroscopic and molecular modeling studies of binding interaction between the new complex of yttrium and 1,10-phenanthroline derivatives with DNA and BSA
Authors: Khorshidi Mahsa , Asadpour Saeid , Aramesh-Boroujeni Zahra , Kooravand Masoumeh , Mobini Dehkordi Maryam
Published: August 2023

In this study, the 4,9 diazafluoren-9-one ligand and [Y(Daf)2Cl3.OH2] complex were synthesized. The interaction of this complex with DNA and bovine serum albumin (BSA) was investigated by UV-vis and fluorescence spectroscopy. The molecular docking method was used to confirm the experimental results, investigate the type of interaction, and determine the binding site. The binding constant and Stern–Volmer constant were calculated using spectroscopy techniques. The binding constant of the Y-complex with DNA and BSA obtained using the UV-vis technique was 1.61 × 105 M−1 and 0.49 × 105 M−1, while that obtained using the fluorescence method was 3.39 × 105 M−1 and 3.63 × 105 M−1, respectively. The results of experimental and theoretical data showed that the interaction between the yttrium complex and DNA and BSA is driven by the hydrogen bond and van der Waals interaction, respectively. The yttrium complex communicates with DNA via the groove interaction. This complex has high binding energy with bovine serum albumin. In addition, the molecular docking results showed that the complex binds to the IIA subdomain of BSA (site I). Finally, anticancer activity of the yttrium complex was studied on MCF-7 and A549 cell lines by using the MTT method. The IC50 values obtained showed that the yttrium complex possesses anticancer activity.
Advancements in ToF-SIMS imaging for life sciences
Authors: Jia Feifei , Zhao Xia , Zhao Yao
Published: August 2023

In the last 2 decades, Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) has gained significant prominence as a powerful imaging technique in the field of life sciences. This comprehensive review provides an in-depth overview of recent advancements in ToF-SIMS instrument technology and its applications in metabolomics, lipidomics, and single-cell analysis. We highlight the use of ToF-SIMS imaging for studying lipid distribution, composition, and interactions in cells and tissues, and discuss its application in metabolomics, including the analysis of metabolic pathways. Furthermore, we review recent progress in single-cell analysis using ToF-SIMS, focusing on sample preparation techniques, in situ investigation for subcellular distribution of drugs, and interactions between drug molecules and biological targets. The high spatial resolution and potential for multimodal analysis of ToF-SIMS make it a promising tool for unraveling the complex molecular landscape of biological systems. We also discuss future prospects and potential advancements of ToF-SIMS in the research of life sciences, with the expectation of a significant impact in the field.
Boosting the inherent activity of NiFe layered double hydroxide via erbium incorporation for water oxidation
Authors: Yang Jitao , Yang Yibin
Published: August 2023

Enhancing the inherent activity of transition metal-based compounds involving Ni and Fe for the electrocatalytic oxygen evolution reaction (OER) is of vital importance, especially NiFe layered double hydroxide (LDH). Here, we doped erbium (Er) into NiFe LDH (Er–NiFe LDH) nanostructures using simple liquid-phase synthesis. The OER activity tests at the same mass loading demonstrated that Er–NiFe LDH has a smaller overpotential and lower Tafel slope than undoped NiFe LDH and commercial RuO2 powders, needing only a small overpotential of 243 mV to achieve a constant current at 10 mA cm-2. Additionally, Er–NiFe LDH was grown in situ on hydrophilic carbon paper substrates (Er–NiFe LDH@CP) to fabricate a three-dimensional (3D) electrode with large catalyst loading, which is favorable for analyzing the stability of morphology structure and elementary components after OER measurement. The galvanostatic measurement suggested that the Er–NiFe LDH@CP electrode possess higher electrochemical durability than a modified glassy carbon electrode due to the stronger mechanical binding between Er–NiFe LDH nanostructures and carbon paper substrate. More importantly, physical characterizations (e.g., SEM and XPS) revealed that Er–NiFe LDH has an excellent stability of morphology, and Ni, Fe, and Er still exist on the catalyst 24 h after the operation. This work provides an effective way for improving the inherent catalytic activity and stability of polymetallic OER catalysts in the future.
Optimizing tight oil extraction from low permeability tight reservoirs: a study on stress sensitivity effects and applications in carbon capture, utilization, and storage
Authors: Jiang Xin , Yu Gaoming
Published: August 2023

This paper takes into consideration the impact of stress sensitivity on bioenergy production from low permeability tight reservoirs, focusing on the post-fracturing phase. This paper established a trilinear flow model that effectively takes into account the dynamics of such reservoirs. This model incorporates stress sensitivity parameters and uses the perturbation transformation and Laplace transformation to solve the productivity prediction formula. The final productivity prediction curve post-fracturing is achieved through a numerical inversion method. This study shows that the stress sensitivity effect significantly diminishes the productivity of fractured horizontal wells used in bioenergy extraction. By introducing stress sensitivity effect parameters, this paper manages to uncover the influence law of mobility and artificial fracture parameters on productivity. Notably, as the reservoir mobility increases, the cumulative bioenergy production from fractured horizontal wells improves significantly. In addition to the above, this paper also scrutinizes the sensitivity of artificial fracture parameters and optimize both the quantity and length of these fractures. This plays a crucial role in enhancing the productivity and efficiency of bioenergy extraction from these tight reservoirs. The applicability and reliability of this method are extensively tested, thereby establishing its potential in guiding the development of low permeability reservoirs post-fracturing. Importantly, this research sets the groundwork for combining bioenergy production with Carbon Capture, Utilization, and Storage (CCUS) technologies. By focusing on optimization and stress management in tight reservoirs, this paper contributes to the sustainable production of bioenergy and reduce carbon emissions, moving a step closer to a cleaner and sustainable future.
The last quarter for sustainable environment in basketball: the carbon footprint of basketball teams in Türkiye and Lithuania
Authors: Vienažindienė Milita , Perkumienė Dalia , Atalay Ahmet , Švagždiene Biruta
Published: August 2023

Today, the sports industry is one of the most important sources of concern due to its negative environmental effects. Especially due to the intense competition schedule, teams and fans have to travel constantly. In this context, the aim of this study, which aims to fill the gap in the literature, is to calculate the carbon footprints of the teams in the Turkish and Lithuanian national basketball leagues based on their travels in the 2021–22 season. The research was limited to Turkey and Basketball national basketball league teams. In the study, the travel distances of the teams in both countries during the 2021–22 basketball season were used as a data set. In the study, the values used in the carbon footprint calculation of 2022 by the United Kingdom Government GHG Conversion Factors for Company Reporting and accepted as the IPCC carbon dioxide emission factor were used. While the carbon footprint, which is obtained by multiplying the emission factor directly by the distance covered by the vehicle type, is presented in tons; The average value calculated for each person was calculated in kg. In the sports sector, basketball is one of the most important sources of transportation-related carbon footprint due to its being one of the team sports and its intense competition schedule. According to the results of this research conducted specifically for Turkey and Lithuania, the total carbon footprint calculated for both countries is 53,029 tons. To make an assessment for both countries, in order to reduce travel based on sports; Arranging league calendars to include less travel, dissemination of green and clean energy-using (electric) vehicles, raising the awareness of club managers, developing environmental assessment policies specific to basketball federations, and increasing cooperation through awareness and training activities seem feasible for sustainable environment and basketball goals.
Pan-glioma analyses reveal species- and tumor-specific regulation of neuron-glioma synapse genes by lncRNAs
Authors: Xiong Wei , Zhang Xuecong , Peng Bin , Zhu Hao , Huang Lijin , He Sha
Published: August 2023

Gliomas are highly heterogeneous and aggressive. Malignant cells in gliomas can contact normal neurons through a synapse-like structure (called neuron-to-glioma synapse, NGS) to promote their proliferation, but it is unclear whether NGS gene expression and regulation show species- and tumor-specificity. This question is important in that many anti-cancer drugs are developed upon mouse models. To address this question, we conducted a pan-glioma analysis using nine scRNA-seq datasets from humans and mice. We also experimentally validated the key element of our methods and verified a key result using TCGA datasets of the same glioma types. Our analyses revealed that NGS gene expression and regulation by lncRNAs are highly species- and tumor-specific. Importantly, simian-specific lncRNAs are more involved in NGS gene regulation than lncRNAs conserved in mammals, and transgenic mouse gliomas have little in common with PDX mouse models and human gliomas in terms of NGS gene regulation. The analyses suggest that simian-specific lncRNAs are a new and rich class of potential targets for tumor-specific glioma treatment, and provide pertinent data for further experimentally and clinically exmining the targets.
Preparation and properties of lightweight and high-strength building ceramsites with oil-based drilling cuttings pyrolysis residues
Authors: Wang Hongjuan , Li Jing , Lin Dong , Jiang Guobing , Zhao Liang , Yu Jinlei , Meng Tian , Huang Xinyu , Liu Hongwu , Yang Yuanyi
Published: August 2023

With the continuing advancement in China’s exploration and development technologies of shale gas resources, there was a significant increase in shale gas oil-based drilling cuttings. In this paper, oil-based drilling cutting pyrolysis residues (ODCPRs) was used as the main raw material for preparing lightweight and high-strength building ceramsites to eliminate the environmental risks and recycle industrial waste. Firstly, orthogonal experiments were used to study the influences of raw material composition, preheating temperature, preheating time, sintering temperature, and sintering time on the properties of building ceramsites. From the results, the sintering temperature and the content of ODCPRs were discovered to be the key factors affecting the sintering process of ceramsites. Secondly, the influences of sintering temperature and heating rate on the properties of building ceramists were further explored to find the optimum sintering conditions. When the content of ODCPRs in the ceramsite was set to be 50%, the obtained building ceramsites presented excellent properties with particle compressive strength of 6.31 MPa, bulk density of 575.11 kg/m3, apparent density of 1097.24 kg/m3, and the water absorption of 1.89%. Finally, XRD, SEM, TG-DSC, and heavy metal leaching experiments were comprehensively conducted to analyze the composition structure variation and sintering mechanism of the building ceramsite. This paper presents an approach for the recycling, utilization, and disposal of oil-based drilling cuttings in the oil field waste management.
Accuracy of the FY-L formula in calculating intraocular lens power after small-incision lenticule extraction
Authors: Hu Yingfeng , Lin Liqun , Zeng Danqi , Wang Yan , Zhang Rong , Zhang Zhe , Wang Zheng , Zhang Guangbin , Ye Xiangyu
Published: August 2023

Purpose

The study aimed to assess the accuracy of the FY-L formula in calculating intraocular lens (IOL) power after small-incision lenticule extraction (SMILE).

Methods

For the post-SMILE IOL calculation of the same eye, the IOL power targeting the pre-SMILE eyes' lowest myopic refractive error was used. The FY-L formula, the Emmetropia Verifying Optical Formula (EVO-L), the Barrett True-K no history, and the Shammas-L, respectively, were used to calculate the predicted refractive error of target IOL power. A comparison was made between the change in spherical equivalent induced by SMILE (SMILE-Dif) and the variance between IOL-Dif (IOL-Induced Refractive Error) before and after SMILE. The prediction error (PE) was defined as SMILE-Dif minus IOL-Dif. The proportion of eyes with PEs within ±0.25 D, ±0.50 D, ±0.75 D, and ±1.00 D, the numerical and absolute prediction errors (PEs and AEs), and the median absolute error (MedAE) were compared.

Results

In total, 80 eyes from 42 patients who underwent SMILE were included in the study. The FY-L formula generated the sample's lowest mean PE (0.06 ± 0.76 D), MAE (0.58 ± 0.50 D), and MedAE (0.47 D), respectively. The PEs in ±0.25 D, ±0.50 D, ±0.75 D, and ±1.00 D comprised 28.8%, 46.3%, 70.0%, and 87.5%, respectively, for the FY-L formula. Compared to other formulas, the FY-L formula produced the highest value with PEs for the percentage of eyes in ±0.50 D, ±0.75 D, and ±1.00 D.

Conclusion

This study demonstrates that the FY-L formula provides satisfactory outcomes in estimating the IOL power in the eyes after SMILE.
Obesity is associated with pain and impaired mobility despite therapy in systemic lupus erythematosus
Authors: Borg Alexander , Lindblom Julius , Gomez Alvaro , Soltani Ameneh , Enman Yvonne , Heintz Emelie , Regardt Malin , Grannas David , Emamikia Sharzad , Parodis Ioannis
Published: August 2023

Objective

To investigate whether abnormal BMI is associated with health-related quality of life (HRQoL) impairments, defined as patient-reported problems within the different dimensions of the three-level EQ-5D (EQ-5D-3L), before and after treatment for active systemic lupus erythematosus (SLE).

Patients and methods

We conducted a post-hoc analysis of data from two phase III clinical trials of belimumab in SLE, i.e., BLISS-52 (n = 865) and BLISS-76 (n = 819). Underweight was defined as BMI <18.5 kg/m2, normal weight as BMI ≥18.5 but <25 kg/m2, pre-obesity as BMI ≥25 but <30 kg/m2, and obesity as BMI ≥30 kg/m2. We investigated associations between BMI groups and problems (level 2 or 3) within each one of the five EQ-5D dimensions before treatment initiation and at week 52, using logistic regression analysis adjusting for age, ethnicity, disease activity, and glucocorticoid dose, and for the post-treatment analysis also for belimumab treatment and baseline EQ-5D-3L responses.

Results

Of 1,684 patients included, 73 (4%) were classified as underweight, 850 (50%) as normal weight, 438 (26%) as pre-obese, and 323 (19%) as obese. At baseline, obesity was associated with mild to severe problems in all EQ-5D dimensions (p < 0.05 for all), yielding the strongest association with problems in mobility (adjusted odds ratio, aOR: 2.1; 95% confidence interval, CI: 1.6–2.8; p < 0.001). Pre-obesity was also associated with problems in mobility (aOR: 1.4; 95% CI: 1.1–1.8; p = 0.005). Post-intervention, obesity was associated with problems in mobility and pain/discomfort, and pre-obesity with problems in mobility and self-care (p < 0.05 for all).

Conclusion

Our study adds to the evidence that high BMI negatively affects SLE patients’ HRQoL, with obesity being associated with pain and impaired mobility despite therapy.
Endothelial APC/PAR1 distinctly regulates cytokine-induced pro-inflammatory VCAM-1 expression
Authors: Birch Cierra A. , Wedegaertner Helen , Orduña-Castillo Lennis B. , Gonzalez Ramirez Monica L. , Qin Huaping , Trejo JoAnn
Published: August 2023

Introduction: Dysfunction of the endothelium impairs its’ protective role and promotes inflammation and progression of vascular diseases. Activated Protein C (APC) elicits endothelial cytoprotective responses including barrier stabilization, anti-inflammatory and anti-apoptotic responses through the activation of the G protein-coupled receptor (GPCR) protease-activated receptor-1 (PAR1) and is a promising therapeutic. Despite recent advancements in developing new Activated protein C variants with clinical potential, the mechanism by which APC/PAR1 promotes different cytoprotective responses remains unclear and is important to understand to advance Activated protein C and new targets as future therapeutics. Here we examined the mechanisms by which APC/PAR1 attenuates cytokine-induced pro-inflammatory vascular cell adhesion molecule (VCAM-1) expression, a key mediator of endothelial inflammatory responses.

Methods: Quantitative multiplexed mass spectrometry analysis of Activated protein C treated endothelial cells, endothelial cell transcriptomics database (EndoDB) online repository queries, biochemical measurements of protein expression, quantitative real-time polymerase chain reaction (RT-qPCR) measurement of mRNA transcript abundance, pharmacological inhibitors and siRNA transfections of human cultured endothelial cells.

Results: Here we report that Activated Protein C modulates phosphorylation of tumor necrosis factor (TNF)-α signaling pathway components and attenuates of TNF-α induced VCAM-1 expression independent of mRNA stability. Unexpectedly, we found a critical role for the G protein-coupled receptor co-receptor sphingosine-1 phosphate receptor-1 (S1PR1) and the G protein receptor kinase-2 (GRK2) in mediating APC/PAR1 anti-inflammatory responses in endothelial cells.

Discussion: This study provides new knowledge of the mechanisms by which different APC/PAR1 cytoprotective responses are mediated through discrete β-arrestin-2-driven signaling pathways modulated by specific G protein-coupled receptor co-receptors and GRKs.
Kinematic analysis of movement patterns during a reach-and-grasp task in stroke patients
Authors: Choi Hyoseon , Park Dongho , Rha Dong-Wook , Nam Hyo Suk , Jo Yea Jin , Kim Deog Young
Published: August 2023

Background

This study aimed to evaluate the kinematic movement patterns during a reach-and-grasp task in post-stroke patients according to the upper extremity impairment severity.

Methods

Subacute stroke patients (n = 46) and healthy controls (n = 20) were enrolled in this study. Spatiotemporal and kinematic data were obtained through 3D motion analysis during the reach-and-grasp task. Stroke patients were grouped using the Fugl-Meyer Assessment (FMA) scale, and a comparison of the groups was performed.

Results

The severe group showed a significantly longer movement time, lower peak velocity, and higher number of movement units than the mild group during the reach-and-grasp task (p < 0.05). Characteristic compensatory movement patterns, such as shoulder abduction, thoracic posterior tilting, and upward and external rotation were significantly greater during the forward transporting phase in the severe group than in the mild group (p < 0.05). The FMA score was significantly associated with the movement time during the forward transporting phase, number of movement units during the reaching phase, range of shoulder abduction-adduction and wrist flexion-extension movements during the reaching phase, and range of thoracic internal-external rotation during the backward transporting phase (p < 0.05).

Conclusion

Post-stroke patients have unique spatiotemporal and kinematic movement patterns during a reach-and grasp-task according to the impairment severity.
Firearm injury prevention counseling for patients with traumatic brain injury: a survey of brain injury physicians
Authors: Ramsay Ian , Miranda-Cantellops Natalia Del Mar , Acosta Oliver , Shapiro Lauren T.
Published: August 2023

Background

Survivors of traumatic brain injury are at increased risk for firearm-related injuries, including suicide.

Aims

To determine current practices of Brain Injury Medicine (BIM) physicians and their rehabilitation teams in assessing patients’ access to firearms and in providing firearm safety education, and the impact of having received training on this topic on physicians’ likelihood of inquiring about patients’ access to firearms.

Methods

14-item web-based cross-sectional survey of 86 U.S. physiatrists board-certified in BIM.

Results

81% of respondents indicated they believe BIM physicians should counsel their patients on firearm safety but only 12.9% reported always doing so. Fifteen percent reported always inquiring about their patients’ access to firearms. 88.2% indicated having never received formal training on firearm injury prevention counseling. Physicians who received such training had 7.5 times higher odds of reporting at least sometimes inquiring about patients’ access to firearms than those who were not trained [95% confidence interval (1.94, 28.64)]. They also had 5.7 times higher odds for reporting being at least moderately comfortable providing patients firearm safety counseling [95% CI: (1.39, 23.22)].

Conclusion

While most BIM specialists who responded to this survey believe they should counsel patients on firearm safety, few always or usually do so. Moreover, most do not routinely inquire about their patients’ access to firearms. The provision of firearm injury prevention training to BIM physicians was strongly associated with an increased likelihood they will inquire about their patients’ access to guns and with an improved comfort level in providing counseling on this subject matter.
Effect of subthalamic coordinated reset deep brain stimulation on Parkinsonian gait
Authors: Bosley Kai M. , Luo Ziling , Amoozegar Sana , Acedillo Kit , Nakajima Kanon , Johnson Luke A. , Vitek Jerrold L. , Wang Jing
Published: August 2023

Introduction

Coordinated Reset Deep Brain Stimulation (CR DBS) is a novel DBS approach for treating Parkinson's disease (PD) that uses lower levels of burst stimulation through multiple contacts of the DBS lead. Though CR DBS has been demonstrated to have sustained therapeutic effects on rigidity, tremor, bradykinesia, and akinesia following cessation of stimulation, i.e., carryover effect, its effect on Parkinsonian gait has not been well studied. Impaired gait is a disabling symptom of PD, often associated with a higher risk of falling and a reduced quality of life. The goal of this study was to explore the carryover effect of subthalamic CR DBS on Parkinsonian gait.

Methods

Three non-human primates (NHPs) were rendered Parkinsonian and implanted with a DBS lead in the subthalamic nucleus (STN). For each animal, STN CR DBS was delivered for several hours per day across five consecutive days. A clinical rating scale modified for NHP use (mUPDRS) was administered every morning to monitor the carryover effect of CR DBS on rigidity, tremor, akinesia, and bradykinesia. Gait was assessed quantitatively before and after STN CR DBS. The stride length and swing speed were calculated and compared to the baseline, pre-stimulation condition.

Results

In all three animals, carryover improvements in rigidity, bradykinesia, and akinesia were observed after CR DBS. Increased swing speed was observed in all the animals; however, improvement in stride length was only observed in NHP B2. In addition, STN CR DBS using two different burst frequencies was evaluated in NHP B2, and differential effects on the mUPDRS score and gait were observed.

Discussion

Although preliminary, our results indicate that STN CR DBS can improve Parkinsonian gait together with other motor signs when stimulation parameters are properly selected. This study further supports the continued development of CR DBS as a novel therapy for PD and highlights the importance of parameter selection in its clinical application.
Deep extreme learning machine with knowledge augmentation for EEG seizure signal recognition
Authors: Zhang Xiongtao , Dong Shuai , Shen Qing , Zhou Jie , Min Jingjing
Published: August 2023

Introduction

Intelligent recognition of electroencephalogram (EEG) signals can remarkably improve the accuracy of epileptic seizure prediction, which is essential for epileptic diagnosis. Extreme learning machine (ELM) has been applied to EEG signals recognition, however, the artifacts and noises in EEG signals have a serious effect on recognition efficiency. Deep learning is capable of noise resistance, contributing to removing the noise in raw EEG signals. But traditional deep networks suffer from time-consuming training and slow convergence.

Methods

Therefore, a novel deep learning based ELM (denoted as DELM) motivated by stacking generalization principle is proposed in this paper. Deep extreme learning machine (DELM) is a hierarchical network composed of several independent ELM modules. Augmented EEG knowledge is taken as complementary component, which will then be mapped into next module. This learning process is so simple and fast, meanwhile, it can excavate the implicit knowledge in raw data to a greater extent. Additionally, the proposed method is operated in a single-direction manner, so there is no need to perform parameters fine-tuning, which saves the expense of time.

Results

Extensive experiments are conducted on the public Bonn EEG dataset. The experimental results demonstrate that compared with the commonly-used seizure prediction methods, the proposed DELM wins the best average accuracies in 13 out of the 22 data and the best average F-measure scores in 10 out of the 22 data. And the running time of DELM is more than two times quickly than deep learning methods.

Discussion

Therefore, DELM is superior to traditional and some state-of-the-art machine learning methods. The proposed architecture demonstrates its feasibility and superiority in epileptic EEG signal recognition. The proposed less computationally intensive deep classifier enables faster seizure onset detection, which is showing great potential on the application of real-time EEG signal classification.
Integrative analysis of mitochondrial metabolic reprogramming in early-stage colon and liver cancer
Authors: Kim Yeongmin , Shin So-Yeon , Jeung Jihun , Kim Yumin , Kang Yun-Won , Lee Sunjae , Oh Chang-Myung
Published: August 2023

Gastrointestinal malignancies, including colon adenocarcinoma (COAD) and liver hepatocellular carcinoma (LIHC), remain leading causes of cancer-related deaths worldwide. To better understand the underlying mechanisms of these cancers and identify potential therapeutic targets, we analyzed publicly accessible Cancer Genome Atlas datasets of COAD and LIHC. Our analysis revealed that differentially expressed genes (DEGs) during early tumorigenesis were associated with cell cycle regulation. Additionally, genes related to lipid metabolism were significantly enriched in both COAD and LIHC, suggesting a crucial role for dysregulated lipid metabolism in their development and progression. We also identified a subset of DEGs associated with mitochondrial function and structure, including upregulated genes involved in mitochondrial protein import and respiratory complex assembly. Further, we identified mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) as a crucial regulator of cancer cell metabolism. Using a genome-scale metabolic model, we demonstrated that HMGCS2 suppression increased glycolysis, lipid biosynthesis, and elongation while decreasing fatty acid oxidation in colon cancer cells. Our study highlights the potential contribution of dysregulated lipid metabolism, including ketogenesis, to COAD and LIHC development and progression and identifies potential therapeutic targets for these malignancies.
Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
Authors: Tacke Charlotte , Bischoff Anne M. , Harb Ali , Vafadari Behnam , Hülsmann Swen
Published: August 2023

The neuronal activity in the respiratory network strongly depends on a variety of different neuromodulators. Given the essential role of astrocytes in stabilizing respiratory network activity generated by neurons in the preBötzinger complex (preBötC), our aim was to investigate astrocytic calcium signaling in the working heart brainstem preparation using fiber-optical imaging. By using transgenic mice that express GCaMP6s specifically in astrocytes, we successfully recorded astrocytic calcium signals in response to norepinephrine from individual astrocytes.
Characteristics of Epicoccum latusicollum as revealed by genomic and metabolic phenomic analysis, the causal agent of tobacco Epicoccus leaf spot
Authors: Li Zhen , Hu Jing-rong , Li Wen-hong , Wang Han-cheng , Guo Zhen-ni , Cheng Xing , Cai Liu-ti , Shi Cai-hua
Published: August 2023

Epicoccum latusicollum is a fungus that causes a severe foliar disease on flue-cured tobacco in southwest China, resulting in significant losses in tobacco yield and quality. To better understand the organism, researchers investigated its optimal growth conditions and metabolic versatility using a combination of traditional methods and the Biolog Phenotype MicroArray technique. The study found that E. latusicollum exhibited impressive metabolic versatility, being able to metabolize a majority of carbon, nitrogen, sulfur, and phosphorus sources tested, as well as adapt to different environmental conditions, including broad pH ranges and various osmolytes. The optimal medium for mycelial growth was alkyl ester agar medium, while oatmeal agar medium was optimal for sporulation, and the optimum temperature for mycelial growth was 25°C. The lethal temperature was 40°C. The study also identified arbutin and amygdalin as optimal carbon sources and Ala-Asp and Ala-Glu as optimal nitrogen sources for E. latusicollum. Furthermore, the genome of E. latusicollum strain T41 was sequenced using Illumina HiSeq and Pacific Biosciences technologies, with 10,821 genes predicted using Nonredundant, Gene Ontology, Clusters of Orthologous Groups, Kyoto Encyclopedia of Genes and Genomes, and SWISS-PROT databases. Analysis of the metabolic functions of phyllosphere microorganisms on diseased tobacco leaves affected by E. latusicollum using the Biolog Eco microplate revealed an inability to efficiently metabolize a total of 29 carbon sources, with only tween 40 showing some metabolizing ability. The study provides new insights into the structure and function of phyllosphere microbiota and highlights important challenges for future research, as well as a theoretical basis for the integrated control and breeding for disease resistance of tobacco Epicoccus leaf spot. This information can be useful in developing new strategies for disease control and management, as well as enhancing crop productivity and quality.
Memory accuracy, suggestibility and credibility in investigative interviews with native and non-native eyewitnesses
Authors: Raver Arman , Lindholm Torun , Gustafsson Philip U. , Alm Charlotte
Published: August 2023

Legal practitioners sometimes obtain eyewitness testimonies from non-native language speakers, yet few studies examine the effects of language in investigative interviews. Here, we investigate how testifying in a non-native vs. native language affects memory accuracy, susceptibility to suggestions, and witnesses perceived credibility. After viewing a mock-crime film, participants in Study 1 (N = 121) testified through (1) free recall, (2) cued recall and (3) the Gudjonsson Suggestibility Scales adapted to the crime scenario used in the present study either in their native (Swedish) or a non-native (English) language. They also rated their confidence in their memories, self-perceived credibility and cognitive effort. Native and non-native witnesses did not differ in memory accuracy, susceptibility to suggestions, self-rated credibility or cognitive effort. Non-native (vs. native) speakers did however report lower confidence in their memories. In Study 2, another group of participants (N = 202) were presented with the testimonies from Study 1, and judged witnesses’ credibility. Non-native witnesses were judged as less credible than native speakers. Thus, while the lower confidence exhibited by non-native eyewitnesses did not correspond to their actual memory accuracy, it influenced observers’ judgments of their performance. The results provide important knowledge for legal practices when evaluating the reliability of testimonies from non-native vs. native speaking eyewitnesses.