bluuebunny/crossref_metadata_2025 huggingface dataset:
Distribution of Glyphosate-Resistance in Echinochloa crus-galli Across Agriculture Areas in the Iberian Peninsula
Authors: Vázquez-García José G. , Rojano-Delgado Antonia M. , Alcántara-de la Cruz Ricardo , Torra Joel , Dellaferrera Ignacio , Portugal João , De Prado Rafael
Published: February 2021

The levels of resistance to glyphosate of 13 barnyard grass (Echinochloa crus-galli) populations harvested across different agriculture areas in the Southern Iberian Peninsula were determined in greenhouse and laboratory experiments. Shikimate accumulation fast screening separated the populations regarding resistance to glyphosate: susceptible (S) E2, E3, E4, and E6 and resistant (R) E1, E5, E7, E8, E9, E10, E11, E12, and E13. However, resistance factor (GR50 E1–E13/GR50 E6) values separated these populations into three groups: (S) E2, E3, E4, and E6, (R) E1, E5, E7, E8, and E9, and very resistant (VR) E10, E11, E12, and E13. 14C-glyphosate assays performed on two S populations (E2 and E6) showed greater absorption and translocation than those found for R (E7 and E9) and VR (E10 and E12) populations. No previous population metabolized glyphosate to amino methyl phosphonic acid (AMPA) and glyoxylate, except for the E10 population that metabolized 51% to non-toxic products. The VR populations showed two times more 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity without herbicide than the rest, while the inhibition of the EPSPS activity by 50% (I50) required much higher glyphosate in R and VR populations than in S populations. These results indicated that different target-site and non-target-site resistance mechanisms were implicated in the resistance to glyphosate in E. crus-galli. Our results conclude that resistance is independent of climate, type of crop, and geographic region and that the level of glyphosate resistance was mainly due to the selection pressure made by the herbicide on the different populations of E. crus-galli studied.
Postharvest Application of 24-Epibrassinolide Reduces Chilling Injury Symptoms and Enhances Bioactive Compounds Content and Antioxidant Activity of Blood Orange Fruit
Authors: Habibi Fariborz , Serrano María , Zacarías Lorenzo , Valero Daniel , Guillén Fabián
Published: February 2021

Blood oranges (Citrus sinensis L. Osbeck cv. Sanguinello) fruit were treated with 24-epibrassinolide (Br) at 1, 5, and 10 μM previous to storage at 5°C during 42 days. The samples were analyzed after 14, 28, and 42 days plus 2 days at 20°C. Chilling injury was reduced in Br-treated fruit based on the lower percentage of electrolyte leakage and visual symptoms of peel dehydration and browning. Treated fruit showed lower acidity losses, due to retention of the main organic acids’ concentration (citric and malic acids), as well as was higher content of sugars (sucrose, fructose, and glucose), especially in those fruit treated with the highest concentration (10 μM). Total phenolics and hydrophilic total antioxidant activity (H-TAA) decreased in control fruit over storage, while Br-treated fruit showed significantly higher concentration. In addition, total anthocyanins were enhanced in Br-treated oranges, which were correlated with color Hue angle. Overall, the application of Br at 10 μM provides results increasing the storability of blood oranges and their content on bioactive compounds with antioxidant activity.
Silicon-Mediated Enhancement of Herbivore Resistance in Agricultural Crops
Authors: Acevedo Flor E. , Peiffer Michelle , Ray Swayamjit , Tan Ching-Wen , Felton Gary W.
Published: February 2021

Silicon (Si) is a beneficial mineral that enhances plant protection against abiotic and biotic stresses, including insect herbivores. Si increases mechanical and biochemical defenses in a variety of plant species. However, the use of Si in agriculture remains poorly adopted despite its widely documented benefits in plant health. In this study, we tested the effect of Si supplementation on the induction of plant resistance against a chewing herbivore in crops with differential ability to accumulate this element. Our model system comprised the generalist herbivore fall armyworm (FAW)Spodoptera frugiperdaand three economically important plant species with differential ability to uptake silicon: tomato (non-Si accumulator), soybean, and maize (Si-accumulators). We investigated the effects of Si supply and insect herbivory on the induction of physical and biochemical plant defenses, and herbivore growth using potted plants in greenhouse conditions. Herbivory and Si supply increased peroxidase (POX) activity and trichome density in tomato, and the concentration of phenolics in soybean. Si supplementation increased leaf Si concentration in all plants. Previous herbivory affected FAW larval weight gain in all plants tested, and the Si treatment further reduced weight gain of larvae fed on Si accumulator plants. Notably, our results strongly suggest that non-glandular trichomes are important reservoirs of Si in maize and may increase plant resistance to chewing herbivores. We conclude that Si offers transient resistance to FAW in soybean, and a more lasting resistance in maize. Si supply is a promising strategy in management programs of chewing herbivores in Si-accumulator plants.
Well-Designed Food Governance as Psychological Mechanism of Consumer Perceptions in the Context of Tourism Poverty Alleviation
Authors: Huang Guo-qing , Lin Kuen-Lin
Published: February 2021

Poverty is a challenge leading to food insecurity in people's minds. This article discusses food governance as a psychological mechanism to facilitate the sense of wellness in people's minds in the context of tourism poverty alleviation. Mainly, we argue that, when a government is implementing tourism poverty alleviation, not only are economic efforts, but also positive psychological feelings are required. We, thus, argue that sound food governance may increase the sense of wellness in the minds of people as food consumers by increasing food safety and security. This perspective paper contributes by explicating the influences of macrolevel governance design of safer and more secure food systems on people's psychological wellness, especially against the background of tourism poverty alleviation in developing countries.
Aesthetically Designing Video-Call Technology With Care Home Residents: A Focus Group Study
Authors: Zamir Sonam , Allman Felicity , Hennessy Catherine Hagan , Taylor Adrian Haffner , Jones Ray Brian
Published: February 2021

Background

Video-calls have proven to be useful for older care home residents in improving socialization and reducing loneliness. Nonetheless, to facilitate the acceptability and usability of a new technological intervention, especially among people with dementia, there is a need for user-led design improvements. The current study conducted focus groups with an embedded activity with older people to allow for a person-centered design of a video-call intervention.

Methods

Twenty-eight residents across four care homes in the South West of England participated in focus groups to aesthetically personalize and ‘dress-up’ the equipment used in a video-call intervention. Each care home was provided with a ‘Skype on Wheels’ (SoW) device, a wheelable ‘chassis’ comprising an iPad or tablet for access to Skype, and a telephone handset. During the focus group, residents were encouraged to participate in an activity using colorful materials to ‘dress-up’ SoW. Comments before, during and after the ‘dress up’ activity were audio recorded. Framework analysis was used to analyze the focus group data.

Results

Older people, including seven with dementia were able to interact with and implement design changes to SoW through aesthetic personalization. Themes arising from the data included estrangement, anthropomorphism, reminiscence, personalization, need for socialization versus fear of socialization and attitudes toward technology. After this brief exposure to SoW, residents expressed the likelihood of using video-calls for socialization in the future.

Conclusion

Care home residents enjoy engaging with new technologies when given the opportunity to interact with it, to personalize it and to understand its purpose. Low cost aesthetic personalization of technologies can improve their acceptability, usability, and implementation within complex care environments.
Factors Influencing Public Panic During the COVID-19 Pandemic
Authors: Nie Xiangtian , Feng Kai , Wang Shengnan , Li Yongxin
Published: February 2021

The coronavirus disease 2019 (COVID-19) pandemic has been regarded as a public health emergency that caused a considerable degree of public panic (such as anxiety and insomnia) during its early stage. Some irrational behaviors (such as excessive search for information related to the pandemic and excessive hoarding of supplies) were also triggered as a result of such panic. Although there has been plenty of news coverage on public panic due to the outbreak, research on this phenomenon has been limited. Since panic is the main psychological reaction in the early stage of the pandemic, which largely determines the level of psychological adaptation, time of psychological recovery, and the incidence of PTSD, there exists a demand to conduct investigation on it. From a public governance perspective, the government’s assessment of public panic may affect the efficiency and effectiveness of pandemic prevention and control. Therefore, it is of obvious practical significance to investigate public panic during the COVID-19 pandemic and analyze its influential factors. The self-compiled COVID-19 Social Mentality Questionnaire was used to collect data from a total of 16,616 participants online, and 13,511 valid responses were received. The results from the chi-square test showed that there were differences in gender, educational level, age, pandemic-related knowledge, self-efficacy, risk level, and objective social support. Furthermore, multiple linear regression analysis results showed that self-efficacy, gender, educational level, age, risk level, pandemic-related knowledge, and objective social support were significant predictors of public panic. Among the research variables, self-efficacy, gender, educational level, and age were negative predictors of panic while risk level, pandemic-related knowledge, and objective social support were positive predictors of panic.
Beyond Justice Perceptions: The Role of Interpersonal Justice Trajectories and Social Class in Perceived Legitimacy of Authority Figures
Authors: Liang Juan , Chen Xiaoyun , Li Tian , Wang Yaxin
Published: February 2021

There is considerable evidence that the experience of justice is associated with perceived legitimacy of authority, but there has been no research about this association when considering past rather than current fairness. Based on the fairness heuristic theory, we tested the hypothesis that interpersonal justice trajectories positively affect perceived legitimacy of the authority; we also tested whether social class moderated this effect. Community residents (N = 111; 54 women) rated the authority's fairness on 16 consecutive weeks and rated perceived legitimacy on the 16th week. The results of latent growth modeling showed that the trajectory of interpersonal justice scores leading up to the final week significantly predicted perceived legitimacy, regardless of the current experience of interpersonal fairness. Tests of moderation showed that the legitimacy perceptions of individuals of lower subjective social class were significantly affected by interpersonal justice trajectories, whereas this was not the case among individuals of higher subjective social class. The results are discussed in terms of their implications for research on perceived legitimacy and justice, as well as their implications for understanding social class.
Relationship Between Psychological Empowerment and the Retention Intention of Kindergarten Teachers: A Chain Intermediary Effect Analysis
Authors: Ma Lina , Zhou Fusheng , Liu Haidan
Published: February 2021

Objective: To investigate the relationship between psychological empowerment, psychological capital, job involvement, and the retention intention of kindergarten teachers in mainland China and the internal mechanism of action.

Methods: A total of 554 kindergarten teachers were investigated by scales for psychological empowerment, psychological capital, job involvement, and retention intention.

Results: (1) Psychological empowerment was positively correlated with psychological capital and job involvement. Psychological capital was positively correlated with job involvement. Psychological empowerment, psychological capital, and job involvement were significantly and positively correlated with retention intention. (2) Psychological empowerment influences kindergarten teachers' retention intention mainly through three indirect effects: the single intermediary effects of psychological capital and job involvement and the chain intermediary effect of psychological capital → job involvement.

Conclusion: Psychological empowerment can not only indirectly predict the retention intention of kindergarten teachers through the single intermediary effects of psychological capital and job involvement, but also indirectly predict the retention intention of kindergarten teachers through the chain intermediary effect of psychological capital and job involvement.
Role of Tactile Noise in the Control of Digit Normal Force
Authors: Naceri Abdeldjallil , Gultekin Yasemin B. , Moscatelli Alessandro , Ernst Marc O.
Published: February 2021

Whenever we grasp and lift an object, our tactile system provides important information on the contact location and the force exerted on our skin. The human brain integrates signals from multiple sites for a coherent representation of object shape, inertia, weight, and other material properties. It is still an open question whether the control of grasp force occurs at the level of individual fingers or whether it is also influenced by the control and the signals from the other fingers of the same hand. In this work, we approached this question by asking participants to lift, transport, and replace a sensorized object, using three- and four-digit grasp. Tactile input was altered by covering participant's fingertips with a rubber thimble, which reduced the reliability of the tactile sensory input. In different experimental conditions, we covered between one and three fingers opposing the thumb. Normal forces at each finger and the thumb were recorded while grasping and holding the object, with and without the thimble. Consistently with previous studies, reducing tactile sensitivity increased the overall grasping force. The gasping force increased in the covered finger, whereas it did not change from baseline in the remaining bare fingers (except the thumb for equilibrium constraints). Digit placement and object tilt were not systematically affected by rubber thimble conditions. Our results suggest that, in each finger opposing thumb, digit normal force is controlled locally in response to the applied tactile perturbation.
Continuity and Discontinuity of Sport and Exercise Type During the COVID-19 Pandemic. An Exploratory Study of Effects on Mood
Authors: Ronkainen Noora J. , Pesola Arto J. , Tikkanen Olli , Brand Ralf
Published: February 2021

Involvement in sport and exercise not only provides participants with health benefits but can be an important aspect of living a meaningful life. The COVID-19 pandemic and the temporary cessation of public life in March/April/May 2020 came with restrictions, which probably also made it difficult, if not impossible, to participate in certain types of sport or exercise. Following the philosophical position that different types of sport and exercise offer different ways of “relating to the world,” this study explored (dis)continuity in the type of sport and exercise people practiced during the pandemic-related lockdown, and possible effects on mood. Data from a survey of 601 adult exercisers, collected shortly after the COVID-19 outbreak in Finland, were analyzed. Approximately one third (35%) of the participants changed their “worldmaking” and shifted to “I–Nature”-type activities. We observed worse mood during the pandemic in those who shifted from “I–Me,” compared to those who had preferred the “I–Nature” relation already before the pandemic and thus experienced continuity. The clouded mood of those experiencing discontinuity may be the result of a temporary loss of “feeling at home” in their new exercise life-world. However, further empirical investigation must follow, because the observed effect sizes were small.
Acquisition of a Joystick-Operated Video Task by Pigs (Sus scrofa)
Authors: Croney Candace C. , Boysen Sarah T.
Published: February 2021

The ability of two Panepinto micro pigs and two Yorkshire pigs (Sus scrofa) to acquire a joystick-operated video-game task was investigated. Subjects were trained to manipulate a joystick that controlled movement of a cursor displayed on a computer monitor. The pigs were required to move the cursor to make contact with three-, two-, or one-walled targets randomly allocated for position on the monitor, and a reward was provided if the cursor collided with a target. The video-task acquisition required conceptual understanding of the task, as well as skilled motor performance. Terminal performance revealed that all pigs were significantly above chance on first attempts to contact one-walled targets (p< 0.05). These results indicate that despite dexterity and visual constraints, pigs have the capacity to acquire a joystick-operated video-game task. Limitations in the joystick methodology suggest that future studies of the cognitive capacities of pigs and other domestic species may benefit from the use of touchscreens or other advanced computer-interfaced technology.
Nutrition as Metabolic Treatment for Anxiety
Authors: Norwitz Nicholas G. , Naidoo Uma
Published: February 2021

Despite the overwhelming prevalence of anxiety disorders in modern society, medications and psychotherapy often fail to achieve complete symptom resolution. A complementary approach to medicating symptoms is to address the underlying metabolic pathologies associated with mental illnesses and anxiety. This may be achieved through nutritional interventions. In this perspectives piece, we highlight the roles of the microbiome and inflammation as influencers of anxiety. We further discuss the evidence base for six specific nutritional interventions: avoiding artificial sweeteners and gluten, including omega-3 fatty acids and turmeric in the diet, supplementation with vitamin D, and ketogenic diets. We attempt to integrate insights from the nutrition science-literature in order to highlight some practices that practitioners may consider when treating individual patients. Notably, this piece is not meant to serve as a comprehensive review of the literature, but rather argue our perspective that nutritional interventions should be more widely considered among clinical psychiatrists. Nutritional psychiatry is in its infancy and more research is needed in this burgeoning low-risk and potentially high-yield field.
Case Report: Parental Loss and Childhood Grief During COVID-19 Pandemic
Authors: Santos Susana , Sá Teresa , Aguiar Inês , Cardoso Inês , Correia Zulmira , Correia Teresa
Published: February 2021

The coronavirus disease 2019 (COVID-19) pandemic is an unprecedent public health crisis, transforming many aspects of our daily life. Protection measures, such as social distancing, nationwide lockdowns, and restrictions on hospital visits and funerals have a serious impact on how people mourn their loved ones. The grieving process during childhood and adolescence evolves along the developmental stages and is a dynamic, non-linear process that needs time. Parental death increases the risk for psychopathology in the short and long term. We present a case of an 11-year-old girl referred to child psychiatry-liaison service by her neurologist due to peer relationship problems and sadness. Fifteen days before her first psychiatric consultation, her father suffered a myocardial infarction complicated with hypoxic ischemic encephalopathy, and he was hospitalized in the intensive care unit. Positive coping mechanisms and adaptive emotional expression strategies were explored during her consultations. Her father died 2 weeks after emergency state and nationwide lockdown was declared in Portugal, during the first COVID-19 outbreak. The family did not have the opportunity for a proper farewell, the funeral obeyed strict rules, and the patient and her family were at home, due to social distancing and school closure policies. Consultations were maintained by telephone calls and, less frequently, by face-to-face appointments. Adaptive and helpful strategies to grieve were shared with the patient and her mother. Intervention with the mother alone was also helpful. Death circumstances related to COVID-19, confinement policies, and social–economical stressors can intensify the grief experience, increasing the risk for complicated grief. Although psychiatric teleconsultation is essential during COVID-19 pandemic, it poses various limitations. Non-verbal communication clues may not be totally apprehended; it may represent a problem in the therapeutic relationship, and access to technology can be difficult for psychiatric patients and clinicians. COVID-19 pandemic policies should include mental health protection measures, which should facilitate adjusted grief responses for those who lose a loved one during this pandemic.
Preparing Graduates for Interprofessional Practice in South Africa: The Dissonance Between Learning and Practice
Authors: Müller Jana , Couper Ian
Published: February 2021

With South Africa's tumultuous history and resulting burden of disease and disability persisting post-democracy in 1994, a proposed decentralization of heath care with an urgent focus on disease prevention strategies ensued in 2010. Subsequently a nationwide call by students to adapt teaching and learning to an African context spoke to the need for responsive health professions training. Institutions of higher education are therefore encouraged to commit to person-centered comprehensive primary health care (PHC) education which equates to distributed training along the continuum of care. To cope with the complexity of patient care and health care systems, interprofessional education and collaborative practice has been recommended in undergraduate clinical training. Stellenbosch University, South Africa, introduced interprofessional home visits as part of the students' contextual PHC exposure in a rural community in 2012. This interprofessional approach to patient assessment and management in an under-resourced setting challenges students to collaboratively find local solutions to the complex problems identified. This paper reports on an explorative pilot study investigating students' and graduates' perceived value of their interprofessional home visit exposure in preparing them for working in South Africa. Qualitative semi-structured individual and focus group interviews with students and graduates from five different health sciences programmes were conducted. Primary and secondary data sources were analyzed using an inductive approach. Thematic analysis was conducted independently by two researchers and revealed insights into effective patient management requiring an interprofessional team approach. Understanding social determinants of health, other professions' roles, as well as scope and limitations of practice in a resource constrained environment can act as a precursor for collaborative patient care. The continuity of an interprofessional approach to patient care after graduation was perceived to be largely dependent on relationships and professional hierarchy in the workplace. Issues of hierarchy, which are often systemic, affect a sense of professional value, efficacy in patient management and job satisfaction. Limitations to using secondary data for analysis are discussed, noting the need for a larger more comprehensive study. Recommendations for rural training pathways include interprofessional teamwork and health care worker advocacy to facilitate collaborative care in practice.
Kidney Exchange Program Reporting Standards: Evidence-Based Consensus From Europe
Authors: Smeulders Bart , Mankowski Michal A. , van de Klundert Joris
Published: February 2021

Background: Kidney Exchange Programs can play an important role to increase access to the life saving and most cost-effective treatment for End Stage Renal Disease. The rise of national KEPs in Europe brings a need for standardized performance reporting to facilitate the development of an international evidence base on program practices.

Methods: We systematically searched and reviewed the literature to extract kidney exchange program performance measures. Reported measures were initially categorized as structure, process, and outcome measures. Expert feedback was used to redefine categories and extend the set of measures to be considered. Using the Delphi method and a panel of 10 experts, the resulting measures were subsequently classified as mandatory (Base set), optional (Extended set), or deleted.

Results: Out of the initial 1,668 articles identified by systematic literature search, 21 European publications on kidney exchange programs were included to collect performance measures, accompanied by three national program reports. The final measurement categories were Context, Population, Enrollment, Matching, Transplantation, and Outcomes. The set of performance measures resulting from the literature review was modified and classified as mandatory or optional. The resulting Base set and Extended set form the kidney exchange program reporting standard.

Conclusions: The evidence-based and consensus-based kidney exchange program reporting standard can harmonize practical and scientific reporting on kidney exchange programs, thus facilitating the advancement of national programs. In addition, the kidney exchange program reporting standard can promote and align cross-national programs.
Ex-Drug Users' and Health Professionals' Perspectives About School-Based Drug Use Prevention Programs: A Qualitative Study
Authors: Tahlil Teuku , Aiyub Aiyub
Published: February 2021

Adolescents have become a prime target for drug dealers in various countries around the world, including in Indonesia. To reduce the high number of drug users amongst adolescents, effective drug prevention programs should be developed and implemented. The present study aimed to identify effective school-based drug prevention programs for adolescents from the perspectives of former drug users and health professionals. This qualitative research used Focus Group Discussions (FGDs) to collect the data. The study participants consisted of eight Ex-Drug Users (EDUs) and eight Health Professionals (HPs) from health educational institutions and health service settings that were selected through purposive sampling. Data analysis was performed using the qualitative content analysis. Five themes were identified from both EDUs and HPs, including the negative effects of drugs, the socialization of drug abuse, the rehabilitation of drug addicts, the partner collaboration, and the obstacles in preventing drug use. All participants agreed that the drug prevention programs such as school-based drug prevention programs are necessary in order to minimize the adverse effects of drug use. While EDUs tended to focus on the social and psychological effects of drugs, the HPs were more inclined toward the risks of diseases, social impacts, and economic problems of the drugs as the reasons for program importance. In terms of the intervention programs, both EDUs and HPs proposed conducting socialization through the use of active methods and agreed that rehabilitation as an effective way for addict students. Also, both groups of participants agreed on the significance of internal and external support, as well as coordination and collaboration to produce an effective and quality program. While the EDUs cited external factors as the main obstacle to the success of program implementation, the HPs mentioned internal factors of the users. School-based drug prevention programs are an important and potentially effective strategy to reduce the use and the effects of drug use among adolescents. The support of various relevant stakeholders is needed to further increase the effectiveness of the programs and eliminate possible barriers to the implementation of the intervention programs.
Clinical Enhancement in AI-Based Post-processed Fast-Scan Low-Dose CBCT for Head and Neck Adaptive Radiotherapy
Authors: Chen Wen , Li Yimin , Yuan Nimu , Qi Jinyi , Dyer Brandon A. , Sensoy Levent , Benedict Stanley H. , Shang Lu , Rao Shyam , Rong Yi
Published: February 2021

Purpose: To assess image quality and uncertainty in organ-at-risk segmentation on cone beam computed tomography (CBCT) enhanced by deep-learning convolutional neural network (DCNN) for head and neck cancer.

Methods: An in-house DCNN was trained using forty post-operative head and neck cancer patients with their planning CT and first-fraction CBCT images. Additional fifteen patients with repeat simulation CT (rCT) and CBCT scan taken on the same day (oCBCT) were used for validation and clinical utility assessment. Enhanced CBCT (eCBCT) images were generated from the oCBCT using the in-house DCNN. Quantitative imaging quality improvement was evaluated using HU accuracy, signal-to-noise-ratio (SNR), and structural similarity index measure (SSIM). Organs-at-risk (OARs) were delineated on o/eCBCT and compared with manual structures on the same day rCT. Contour accuracy was assessed using dice similarity coefficient (DSC), Hausdorff distance (HD), and center of mass (COM) displacement. Qualitative assessment of users’ confidence in manual segmenting OARs was performed on both eCBCT and oCBCT by visual scoring.

Results: eCBCT organs-at-risk had significant improvement on mean pixel values, SNR (p < 0.05), and SSIM (p < 0.05) compared to oCBCT images. Mean DSC of eCBCT-to-rCT (0.83 ± 0.06) was higher than oCBCT-to-rCT (0.70 ± 0.13). Improvement was observed for mean HD of eCBCT-to-rCT (0.42 ± 0.13 cm) vs. oCBCT-to-rCT (0.72 ± 0.25 cm). Mean COM was less for eCBCT-to-rCT (0.28 ± 0.19 cm) comparing to oCBCT-to-rCT (0.44 ± 0.22 cm). Visual scores showed OAR segmentation was more accessible on eCBCT than oCBCT images.

Conclusion: DCNN improved fast-scan low-dose CBCT in terms of the HU accuracy, image contrast, and OAR delineation accuracy, presenting potential of eCBCT for adaptive radiotherapy.
Evaluation of Sustainability Determinants of Small Farming Systems via Participatory Modeling and Fuzzy Multi-Criteria Processes: The Case Study of Heliciculture in Greece
Authors: Hatziioannou Marianthi , Kokkinos Konstantinos
Published: February 2021

Feasibility and sustainability of small-scale farming systems are the most critical factors that determine future investments of urban agro-farming while promoting eco-systematic livelihood development and biodiversity. The determination and the diversification of the ambivalent and heterogeneous factors that affect the investment decision making of small farming systems is studied in this work via the application of participatory modeling methodology of regional stakeholders. Defuzzification of the participants' categorical feedback is succeeded applying Fuzzy Analytical Hierarchy Process (F-AHP) to set the pairwise weight matrix of the determinants suggested by the stakeholders. At a second phase, an innovative Fuzzy Cognitive Map (FCM) model is utilized to determine the weighted inter-causalities between all agricultural, environmental, and socio-economic criteria from the F-AHP output. Apart from the concept circumscription, we use the FCM to provide an in-depth scenario analysis including steady state and dynamic evaluation of driver concepts to receiver concepts in the model. The methodology is applied for the case study of heliciculture farming systems in Greece. Results show that sustainability is affected primarily by agricultural and inventory factors and secondarily by social and environmental factors identifying such cradle-to-farm gate life cycle assessment to be of low carbon footprint.
Interactive Audio Augmented Reality in Participatory Performance
Authors: Nagele Anna N. , Bauer Valentin , Healey Patrick G. T. , Reiss Joshua D. , Cooke Henry , Cowlishaw Tim , Baume Chris , Pike Chris
Published: February 2021

Interactive Audio Augmented Reality (AAR) facilitates collaborative storytelling and human interaction in participatory performance. Spatial audio enhances the auditory environment and supports real-time control of media content and the experience. Nevertheless, AAR applied to interactive performance practices remains under-explored. This study examines how audio human-computer interaction can prompt and support actions, and how AAR can contribute to developing new kinds of interactions in participatory performance.This study investigates an AAR participatory performance based on the theater and performance practice by theater maker Augusto Boal. It draws from aspects of multi-player audio-only games and interactive storytelling. A user experience study of the performance shows that people are engaged with interactive content and interact and navigate within the spatial audio content using their whole body. Asymmetric audio cues, playing distinctive content for each participant, prompt verbal and non-verbal communication. The performative aspect was well-received and participants took on roles and responsibilities within their group during the experience.
The Role of Basin Geometry in Mountain Snowpack Responses to Climate Change
Authors: Shea Joseph M. , Whitfield Paul H. , Fang Xing , Pomeroy John W.
Published: February 2021

Snowmelt contributions to streamflow in mid-latitude mountain basins typically dominate other runoff sources on annual and seasonal timescales. Future increases in temperature and changes in precipitation will affect both snow accumulation and seasonal runoff timing and magnitude, but the underlying and fundamental roles of mountain basin geometry and hypsometry on snowmelt sensitivity have received little attention. To investigate the role of basin geometry in snowmelt sensitivity, a linear snow accumulation model and the Cold Regions Hydrological Modeling (CRHM) platform driven are used to estimate how hypsometry affects basin-wide snow volumes and snowmelt runoff. Area-elevation distributions for fifty basins in western Canada were extracted, normalized according to their elevation statistics, and classified into three clusters that represent top-heavy, middle, and bottom-heavy basins. Prescribed changes in air temperature alter both the snow accumulation gradient and the total snowmelt energy, leading to snowpack volume reductions (10–40%), earlier melt onsets (1–4 weeks) and end of melt season (3 weeks), increases in early spring melt rates and reductions in seasonal areal melt rates (up to 50%). Basin hypsometry controls the magnitude of the basin response. The most sensitive basins are bottom-heavy, and have a greater proportion of their area at low elevations. The least sensitive basins are top-heavy, and have a greater proportion of their area at high elevations. Basins with similar proportional areas at high and low elevations fall in between the others in terms of sensitivity and other metrics. This work provides context for anticipating the impacts of ongoing hydrological change due to climate change, and provides guidance for both monitoring networks and distributed modeling efforts.
The Role Played by Public Universities in Mitigating the Coronavirus Catastrophe in Brazil: Solidarity, Research and Support to Local Governments Facing the Health Crisis
Authors: Arrais Cristiano Alencar , Corcioli Graciella , Medina Gabriel da Silva
Published: February 2021

This study aims to assess the impacts of the Covid-19 pandemic in Brazil and how it has been dealt with by both the government and in civil society. To this end, we examine the Brazilian public health system and the measures taken by the Bolsonaro Government that led to Brazil being ranked second in overall Covid-19 infections in the world through August 2020. In the absence of national leadership facing the Covid-19 health crisis, we list a set of science-based initiatives promoted by Brazilian public universities in connection with local governments, NGOs and communities as a means of mitigating the consequences and spread of the pandemic. This study is based on the consultation of institutional material published by universities summarizing their research and outreach initiatives. Results reveal that university initiatives included: 1) Alerts to society on the risks of the pandemic, with an emphasis on establishing observatories that assisted local governments and civil society in understanding the evolution of the disease, as well as in implementing measures for its prevention; 2) Direct assistance to local communities, with emphases on the addition of beds in university hospitals for treating patients with Covid-19 and on the manufacturing of personal protective equipment and; 3) Research to find solutions to prevent and treat the disease, with emphases on the development of tests for Covid-19, as well as on carrying out phase 3 vaccine trials. Through these measures, Brazilian public Federal Universities played a key role in supporting both civil society and local governments in mitigating the impacts of the pandemic.
Yield and Production Components of Corn Under Straw of Marandu Palisade Grass Inoculated With Azospirillum brasilense in the Low-Land Cerrado
Authors: Modesto Viviane Cristina , Andreotti Marcelo , Nakao Allan Hisashi , Soares Deyvison de Asevedo , Froio Leonardo de Lima , Dickmann Lourdes , Pascoaloto Isabô Melina , Fernandes Isabela Maria Dias Moyses
Published: February 2021

The use of plant growth-promoting bacteria combined with Integrated Agricultural Production Systems (IAPS) has resulted in productivity increases in the Brazilian Cerrado region. Thus, the objective was to evaluate the effect of inoculating or not inoculating Azospirillum brasilense on corn and palisade grass seeds (Urochloa brizantha cv. Marandu) on growth, yield components, and dry matter yield of the aerial part and grains in an Oxisol cropped in ICLS and under a no-tillage system for 12 years in an irrigated area. The experimental design was a randomized block design with four replications. The treatments were composed of corn (monoculture) with inoculated seed, corn (monoculture) without inoculated seed, corn + palisade grass (intercropping) without inoculation, corn + palisade grass (intercropping) with inoculation in both seeds, corn + palisade grass (intercropping) with inoculation of corn seeds, and corn + palisade grass (intercropping) with inoculation of grass seeds. At the end of each production cycle, the yield and components of corn, corn straw biomass, and dry matter of palisade grass were evaluated. Considering the optimal conditions of soil provided by liming and fertilization at sowing and nitrogen applied in topdressing, the application of A. brasilense in corn seeds did not show its potential. Intercropped systems inoculated or not with A. brasilense on corn seeds increase grain yield, dry matter production of grass, and nutrient accumulation in the straw, providing greater sustainability to the Cerrado no-tillage system.
Local Markets: Agrobiodiversity Reservoirs and Access Points for Farmers' Plant Propagation Materials
Authors: Heindorf Claudia , Reyes-Agüero Juan Antonio , van't Hooft Anuschka
Published: February 2021

Local markets are access points to local agrobiodiversity and to part of the informal seed systems on which most small-scale farmers worldwide depend. With the urgent need for more sustainable food systems, detailed studies of the food plant diversity in local markets contribute to a better understanding of the role of local markets in a functioning rural food system. In particular, the products that farmers trade and also use for plant propagation are of interest, i.e., seeds and other propagules such as cuttings, pseudostems, rhizomes, or tubers purposes, since they represent our genetic capital for food production. This study aims to show the role of local markets as access points for plant propagation materials and their contribution to regional in situ conservation of local food plant resources. We analyzed the inter- and intra-specific food plant diversity of the products from local merchants in 10 markets in the agrobiodiversity rich region of the Huasteca Potosina, Mexico. We recorded 275 different food plants consisting of 99 plant species, which have a high intraspecific richness of 210 variants. The list includes 58 species that are useful for propagation. The average number of variants suitable for propagation at each market is 58.4. The results show that the different richness parameters vary within and between the inventoried markets. They correlate partially to different factors like market size and origin. We conclude that local markets in the Huasteca Potosina are important components of the rural food system by providing access to a great variety of local food plants, as well as to seeds and other propagation materials for farming. However, diversity may be threatened, because of the high average proportion of unique and rare food plants (63.5%) in the markets. Also, almost half (45.1%) of the total richness is present in <1% of the inventoried stands. Political actions are needed to maintain and promote the use and conservation of this diversity in the future.
Chemical Compositions of Edamame Genotypes Grown in Different Locations in the US
Authors: Yu Dajun , Lin Tiantian , Sutton Kemper , Lord Nick , Carneiro Renata , Jin Qing , Zhang Bo , Kuhar Thomas , Rideout Steven , Ross Jeremy , Duncan Susan , Yin Yun , Wang Hengjian , Huang Haibo
Published: February 2021

The consumption of edamame [Glycine max (L.) Merr.] in the US has rapidly increased due to its nutritional value and potential health benefits. In this study, 10 edamame genotypes were planted in duplicates in three different locations in the US—Whitethorne, Virginia (VA), Little Rock, Arkansas (AR), and Painter, VA. Edamame samples were harvested at the R6 stage of the bean development when beans filled 80–90% of the pod cavity. Afterward, comprehensive chemical composition analysis, including sugars, alanine, protein, oil, neutral detergent fiber (NDF), starch, ash, and moisture contents, were conducted on powdered samples using standard methods and the total sweetness was calculated based on the measured sugars and alanine contents. Significant effects of the location were observed on all chemical constituents of edamame (p < 0.05). The average performance of the genotypes was higher in Whitethorne for the contents of free sucrose (59.29 mg/g), fructose (11.42 mg/g), glucose (5.38 mg/g), raffinose (5.32 mg/g), stachyose (2.34 mg/g), total sweetness (78.63 mg/g), and starch (15.14%) when compared to Little Rock and Painter. The highest soluble alanine (2.67 mg/g), NDF (9.00%), ash (5.60%), and moisture (70.36%) contents were found on edamame planted in Little Rock while edamame planted in Painter had the highest crude protein (43.11%) and oil (20.33%) contents. Significant effects of genotype were observed on most of the chemical constituents (p < 0.05) except NDF and raffinose. Among the 10 genotypes, R13-5029 consistently had high sucrose content and total sweetness across the three locations, meanwhile it had relatively high protein and fiber contents. Overall, the results indicate that to breed better edamame genotypes in the US, both genotype and planting location should be taken into considerations.
Case Report: Small Bowel Obstruction Owing to Self-Anchoring Barbed Suture Device After TAPP Repair
Authors: Zheng Longbo , Yin Xiangyi , Liu Huasheng , Wang Shouguang , Hu Jilin
Published: February 2021

Groin hernioplasty is the most performed intervention in the adults worldwide, the minimally invasive inguinal hernia repair techniques widely used by surgeons today are transabdominal preperitoneal patch plasty (TAPP) and total extraperitoneal patch plasty (TEP). We report a 62-year-old man with bowel obstruction caused by the use of self-anchoring barbed suture to close the peritoneum 3 days after TAPP. Surgeons using the barbed suture should be alert to this possibility when encountering this complication of intestinal obstruction, so as to avoid more serious consequences.
An Attenuated Escherichia coli K88ac LT(S63K)ΔSTb Efficiently Provides Protection Against Enterotoxigenic Escherichia coli in the Mouse Model
Authors: Zhang Xinyu , Yu Shupei , Cheng Darong , Feng Yu , Yang Yuefei , Sun Huaichang , Ding Jiabo , Wang Fang
Published: February 2021

To develop an attenuated vaccine candidate against K88ac enterotoxigenic Escherichia coli (ETEC), a novel Escherichia coli (E. coli) K88ac LT(S63K)ΔSTb with LT(S63K) mutation and ST1 deletion was generated using site mutagenesis and λ-Red homologous recombination based on wild paternal ETEC strain C83902. E. coli K88ac LT(S63K)ΔSTb showed very similar fimbriae expression and growth kinetics to the wild strain C83902, but it was significantly attenuated according to the results of a rabbit ligated ileal loop assay and mouse infection study. Oral inoculation with E. coli K88ac LT(S63K)ΔSTb stimulated the mucosa immune response and induced the secretion of IgA to K88ac in the intestines in mice. A challenge experiment revealed that the attenuated strain provided efficient protection against C83902 in the following 7 days and at the 24th day post-inoculation, suggesting that the attenuated isolate could act as an ecological protectant and vaccine in preventing K88ac ETEC.
A Novel Real-Time PCR Assay for the Rapid Detection of Virulent Streptococcus equi Subspecies zooepidemicus—An Emerging Pathogen of Swine
Authors: Kuchipudi Suresh V. , Surendran Nair Meera , Yon Michele , Gontu Abhinay , Nissly Ruth H. , Barry Rhiannon , Greenawalt Denver , Pierre Traci , Li Lingling , Thirumalapura Nagaraja , Tewari Deepanker , Jayarao Bhushan
Published: February 2021

Streptococcus equi subspecies zooepidemicus, a zoonotic bacterial pathogen caused a series of outbreaks with high mortality affecting swine herds in multiple locations of the USA and Canada in 2019. Further genetic analysis revealed that this agent clustered with ATCC 35246, a S. zooepidemicus strain associated with high mortality outbreaks in swine herds of China originally reported in 1977. Rapid and accurate diagnosis is absolutely critical for controlling and limiting further spread of this emerging disease of swine. Currently available diagnostic methods including bacteriological examination and PCR assays do not distinguish between the virulent strains and avirulent commensal strains of S. zooepidemicus, which is critical given that this pathogen is a normal inhabitant of the swine respiratory tract. Based on comparative analyses of whole genome sequences of the virulent isolates and avirulent sequences, we identified a region in the SzM gene that is highly conserved and restricted to virulent S. zooepidemicus strains. We developed and validated a novel probe-based real-time PCR targeting the conserved region of SzM. The assay was highly sensitive and specific to the virulent swine isolates of Streptococcus equi subspecies zooepidemicus. No cross reactivity was observed with avirulent S. zooepidemicus isolates as well as other streptococcal species and a panel of porcine respiratory bacterial and viral pathogens. The PCR efficiency of the assay was 96.64 % and was able to detect as little as 20 fg of the bacterial DNA. We then validated the diagnostic sensitivity and specificity of the new PCR assay using a panel of clinical samples (n = 57) and found that the assay has 100% sensitivity and specificity as compared to bacteriological culture method. In summary, the PCR assay will be an extremely valuable tool for the rapid accurate detection of virulent swine S. zooepidemicus isolates and directly from clinical samples.