bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Ganoderma lucidum (Curtis) P. Karst. Supplementation on the Technological, Chemical, and Quality Parameters of Wheat Bread
Authors: Łysakowska Paulina , Sobota Aldona , Wirkijowska Anna , Zarzycki Piotr , Blicharz-Kania Agata
Published: September 2024

This study explores the incorporation of Ganoderma lucidum (Curtis) P. Karst. (Reishi mushroom) into wheat bread to develop a functional food with enhanced nutritional value. Reishi powder was added to bread formulations at levels of 3%, 6%, 9%, and 12% to assess its effects on physicochemical, nutritional, and sensory properties. The 12% Reishi supplementation resulted in a twofold increase in total dietary fibre (from 7.21 g to 17.08 g per 100 g dry matter) and significant (p < 0.05) elevations in mineral content, particularly calcium (68%), iron (32%), and manganese (61.9%). Carbohydrate content decreased markedly by 27%, contributing to a 19.33% reduction in caloric value. Reishi addition improved bread yield and reduced baking losses, enhancing production efficiency. However, higher Reishi levels negatively impacted bread volume, possibly due to interference with gluten network formation. An increase in crumb moisture was observed, contributing to extended freshness. Sensory evaluation revealed that loaves of bread containing up to 6% Reishi were acceptable to consumers, whereas higher levels detrimentally affected flavour and aroma. Therefore, Reishi-enriched bread, particularly with 6% supplementation, presents a promising functional alternative to conventional wheat bread, optimising nutritional benefits while maintaining consumer acceptability.
Investigation of Salmonella enteritidis Growth under Varying Temperature Conditions in Liquid Whole Egg: Proposals for Smart Management Technology for Safe Refrigerated Storage
Authors: Baek Seung-Hee , Lim Chang-Geun , Park Jung-Il , Seo Yeon-Beom , Nam In-Sik
Published: September 2024

This study investigates the growth characteristics of Salmonella enteritidis (S. enteritidis) in liquid whole egg under both isothermal and non-isothermal storage conditions to understand the risks associated with inadequate temperature management in the egg industry. Using controlled laboratory simulations, liquid whole egg samples inoculated with S. enteritidis were stored under various isothermal (5, 15, 25, 35, and 45 °C) and non-isothermal conditions (5–10, 15–20, 25–30, 35–40, and 45–50 °C). The growth behavior of the S. enteritidis was analyzed using a two-step predictive modeling approach. First, growth kinetic parameters were estimated using a primary model, and then the effects of temperature on the estimated specific growth rate and lag time were described using a secondary model. Independent growth data under both isothermal and non-isothermal conditions were used to evaluate the models. The results showed that S. enteritidis exhibits different growth characteristics depending on temperature conditions, emphasizing the need for strict temperature control to prevent foodborne illnesses. To address this, a predictive growth model tailored for non-isothermal conditions was developed and validated using experimental data, demonstrating its reliability in predicting S. enteritidis behavior under dynamic temperature scenarios. Additionally, temperature management technologies were proposed and tested to improve food safety during refrigerated storage. This study provides a scientific basis for improving food safety protocols in the egg industry, thereby protecting public health and maintaining consumer confidence amid temperature fluctuations.
Dynamic Change of Volatile Fatty Acid Derivatives (VFADs) and Their Related Genes Analysis during Innovative Black Tea Processing
Authors: Zhou Zi-Wei , Wu Qing-Yang , Wu Yang , Deng Ting-Ting , Li Yu-Qing , Tang Li-Qun , He Ji-Hang , Sun Yun
Published: September 2024

Volatile fatty acid derivatives (VFADs) play a significant role in contributing to flowery–fruity flavor black tea. Innovative black tea is typically crafted from aroma-intensive tea cultivars, such as Jinmudan, using defined production methodologies. In this study, the during-processing tea leaves of innovative black tea were applied as materials, and we selected a total of 45 VFADs, comprising 11 derived aldehydes, nine derived alcohols, and 25 derived esters. Furthermore, the dynamic variations of these VFADs were uncovered. Transcriptome analysis was performed to identify genes involved in the LOX (lipoxygenase) pathway, resulting in the identification of 17 CsLOX genes, one hydrogen peroxide lyase (CsHPL) gene, 11 alcohol dehydrogenases (CsADH) genes, 11 genes as acyl CoA oxidase (CsACOX) genes, and three allene oxide synthase (CsAOS) genes. Additionally, the expression levels of these genes were measured, indicating that the processing treatments of innovative black tea, particularly turn-over and fermentation, had a stimulation effect on most genes. Finally, qRT-PCR verification and correlation analysis were conducted to explain the relationship between VFADs and candidate genes. This study aims to provide a reference for illuminating the formation mechanisms of aroma compounds in innovative black tea, thereby inspiring the optimization of innovative processing techniques and enhancing the overall quality of black tea.
Factors Driving Individuals’ Attitudes toward Sugar and Sweet-Tasting Foods: An Analysis within the Scope of Theory of Planned Behavior
Authors: Kural Enç Hatice Pınar , Kahrıman Meryem , Gençalp Cansu , Yılmaz Salim , Köse Gizem , Baş Murat
Published: September 2024

Public health authorities are undertaking initiatives aimed at reducing sugar intake because it is linked to detrimental health outcomes. Individuals’ attitudes and intentions toward sugar can be significant factors affecting intake. Therefore, we here aimed to evaluate individuals’ attitudes and intentions toward sugar intake by combining the theory of planned behavior with different frameworks. Accordingly, we developed an online questionnaire and reached 940 participants. We observed that subjective norms (β = −0.140; p < 0.001) and perceived behavioral control (β = −0.138; p < 0.001) as defined in the theory of planned behavior significantly affected attitude. Subsequently, examining the effect of attitude (β = −0.209; p = 0.018) and intention (β = 0.717; p < 0.001) on sugar intake showed that intention had a positive effect, whereas attitude had no significant effect. Mediation analysis showed that attitude influenced sugar intake through intention (β = −0.286; p = 0.001). Furthermore, we determined that components including habits, perceived understanding, perceived nonautonomy, negativity, and apathy can affect attitude toward sugar intake (p < 0.001). Analyzing the effects of sociodemographic factors revealed that sugar intake was lower in individuals with food allergies (p < 0.05). In conclusion, these findings suggest that the theory of planned behavior, when combined with different frameworks, can be effective in predicting sugar intake and individuals’ intentions.
Metagenomic and Metabolomic Profiling Reveals the Differences of Flavor Quality between Hongqu Rice Wines Fermented with Gutian Qu and Wuyi Qu
Authors: Liang Zihua , Chen Shiyun , Wang Hao , Wu Qi , Guo Weiling , Ni Li , Lv Xucong
Published: September 2024

Jiuqu (starter) makes an important contribution to the formation of the flavor characteristics of Hongqu rice wine (HQW). Gutian Qu (GTQ) and Wuyi Qu (WYQ) are two kinds of Jiuqu commonly used in HQW brewing, but the comparison of the two kinds of HQW is still insufficient at present. The objective of this study was to compare the dynamic changes of amino acids (AAs), higher alcohols (HAs), bioamines (BAs), volatile flavor compounds (VFCs), and microbial communities in HQW fermentation, with GTQ and WYQ as starter. This study used an automatic amino acid analyzer, GC, HPLC, and GC-MS to detect AAs, HAs, Bas, and VFCs during fermentation; metagenomic sequencing technology was used to elucidate the microbial community and its functional characteristics. The results showed that the contents of AAs and HAs in HQW brewed with WYQ (WYW) were significantly higher than those in HQW brewed with GTQ (GTW). On the contrary, the majority of BAs in GTW were significantly higher than those in WYW. The composition of VFCs in WYW and GTW were obviously different, as most of the VFCs were notably enriched in WYW, while ethyl caproate, isoamyl acetate, ethyl heptanoate, ethyl nonanoate, 1-decanol, citronellol, phenethyl acetate, and hexanoic acid were more abundant in GTW. Burkholderia gladioli, Pantoea dispersa, Weissella cibaria, Monascus purpureus, and Saccharomyces cerevisiae were the predominant microbial populations in GTW brewing at the species level, while Sphingomonas sp., Kosakonia cowanii, Enterobacter asburiae, Leuconostoc lactis, Aspergillus niger, and Saccharomyces cerevisiae were the dominant microbial species in WYW brewing. The abundance of functional genes involved in BAs biosynthesis were much higher in GTW brewing, while the abundance of functional genes related to the metabolism of characteristic VFCs were much higher in WYW brewing. Collectively, these findings provided evidence for elucidating the effects of Jiuqu and microbial communities on HQW flavor quality, and laid a solid foundation for the improvement of HQW flavor quality.
A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk
Authors: Shang Yuhan , Zhang Dandan , Shen Yun , Pan Yuanhu , Wang Jing , Wang Yulian
Published: September 2024

Prolonged consumption of animal-derived foods containing high levels of lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry to predict the efficacy of LIN haptens prior to chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which is specific to LIN. The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited high specificity for detecting LIN residues, with an IC50 value of 0.57 ± 0.03 µg/kg. The method effectively detected LIN residues in pork and milk samples, achieving a limit of detection (LOD) ranging from 0.81 to 1.20 µg/kg and a limit of quantification (LOQ) ranging from 2.09 to 2.29 µg/kg, with recovery rates between 81.9% and 108.8%. This study offers a valuable tool for identifying LIN residues in animal-derived food products. Furthermore, the efficient hapten prediction method presented herein improves antibody preparation efficiency and provides a simple method for researchers in screening haptens.
Different Combinations of Nitrogen and Carbon Sources Influence the Growth and Postbiotic Metabolite Characteristics of Lactiplantibacillus plantarum Strains Isolated from Malaysian Foods
Authors: Zheng Qinri , Chia Suet Lin , Saad Norazalina , Song Adelene Ai-Lian , Loh Teck Chwen , Foo Hooi Ling
Published: September 2024

Postbiotic metabolites produced by Lactiplantibacillus plantarum strains isolated from Malaysian food have been extensively reported for their positive effects on health. Understanding the effects of different combinations of carbon and nitrogen sources on the growth and corresponding characteristics of postbiotic metabolites produced by different strains of L. plantarum is important for various applications. Hence, the effects of different combinations of carbon (glucose, lactose, sucrose and dextrose) and nitrogen (X-SEED Kat, X-SEED Peptone, X-SEED Nucleo Advanced, Nucel875 MG, FM888 and FM902) sources on the growth of six strains of L. plantarum (RG11, RG14, RI11, RS5, TL1 and UL4) and the functional characteristics (bacteriocin inhibitory activity, antioxidant activity and lactic acid concentration) of their respective postbiotic metabolites were investigated in this study. UL4 produced the highest viable cell population with sucrose and Nucel875 nitrogen source. The UL4 strain also produced the strongest bacteriocin inhibitory activity with dextrose and FM888 nitrogen source. In comparison, the RI11 strain produced the highest lactic acid concentration with dextrose and Nucel875 nitrogen source and the highest reducing power of RS5 and TL1 postbiotic metabolites was achieved with MRS medium. In the combination of sucrose and X-Seed KAT nitrogen source, RG14 produce the highest hydroxyl radical scavenging activity. The effects of different combinations of carbon and nitrogen sources on the viable cell population of L. plantarum strains and the respective functional characteristics of postbiotic metabolites were strain dependent. The current study also revealed that fermentation media were an important factor that greatly impacted the functionalities of postbiotic metabolites due to the presence of various bioactive compounds that contributed to high antioxidant and antimicrobial properties. The results of this study will facilitate the subsequent medium design and optimisation for the development and production of specific postbiotic metabolites produced by the respective L. plantarum strain for their applications in various industries.
Characterization of a Novel Acid-Stable Chitosanase from Lentinula edodes Suitable for Chitooligosaccharide Preparation
Authors: Wang Yanxin , Zhao Yujie , Li Jingchen , Zong Haobo , Chen Ying , Zhou Jinyu , Li Xinlian , Ye Xianfeng
Published: September 2024

As high-value chitosan derivatives, chitooligosaccharides (COSs) with biodegradable, biocompatible, nontoxic, antimicrobial, and antioxidant activities have been widely applied in food-related fields. Chitosanases can hydrolyze chitosan to produce COSs. Herein, a chitosanase (LeCho1) from Lentinula edodes was successfully expressed in Escherichia coli and was then purified and characterized. LeCho1 had a low sequence identity with other chitosanases reported from the GH75 family. The recombinant protein showed a molecular mass of 27 kDa on SDS-PAGE. LeCho1 preferentially hydrolyzed chitosan with a high degree of deacetylation (DDA) and exhibited maximal activity (71.88 U/mg) towards 95% DDA chitosan at pH 3.0 and 50 °C. It possessed good stability at pH 2.0–6.0 and temperatures below 45 °C. Its hydrolytic activity was remarkably enhanced by the metal ion Mn2+ at 1 mM, while it was totally inhibited by 1 mM Fe3+ or 10 mM EDTA. Its Km and Vmax values were 0.04 μM and 76.81 μmol·min−1·mg−1, respectively, indicating good substrate affinity. LeCho1 degraded chitosan into COSs with degrees of polymerization (DPs) of 2–5, while it had no action on COSs with DPs of less than 5, revealing its endo-chitosanase activity. This study proved that chitosanase LeCho1 is a promising candidate in the industrial preparation of COSs due to its excellent properties.
Effects of a Sorghum Beverage with Lacticaseibacillus paracasei on Body Composition, Lipid Profiles, and Intestinal Health in Overweight and Obese Adults: A Randomized Single-Blind Pilot Study
Authors: da Silva Lucimar Aguiar , de São José Vinícius Parzanini Brilhante , Rodrigues Larissa Arruda , do Prado Pietra Vidal Cardoso , Toledo Renata Celi Lopes , de Barros Frederico Augusto Ribeiro , de Souza Andressa Moreira , Antoniassi Rosemar , de Carvalho Carlos Wanderlei Piler , Queiroz Valéria Aparecida Vieira , dos Santos Karina Maria Olbrich , Pierre Joseph Francis , da Silva Bárbara Pereira , Martino Hércia Stampini Duarte
Published: September 2024

(1) Background: This study aimed to evaluate the effect of an extruded whole-grain sorghum beverage containing L. paracasei on body composition, lipid profiles, and intestinal health in overweight and obese adults. (2) Methods: A chronic, single-blind randomized controlled pilot study was conducted with 30 volunteers allocated to three groups (n = 10/group): extruded sorghum beverage (ESB), extruded sorghum beverage with L. paracasei (ESPB), and control beverage (CB) (waxy maize starch). The chemical composition of the beverages was analyzed. Volunteers consumed the beverages for ten weeks at breakfast, along with individual dietary prescriptions. Body composition, biochemical markers, gastrointestinal symptoms, stool consistency, intestinal permeability, short-chain fatty acids, fecal pH, and stool L. paracasei DNA concentration were analyzed at the beginning and end of the intervention period. (3) Results: The ESB showed better composition than the CB, particularly in terms of resistant starch content, total phenolic compounds, condensed tannins, and antioxidant capacity. Both the ESB and the ESPB had an effect on body composition (estimated total visceral fat and waist volume), biochemical markers (Castelli index I), and intestinal health (Bristol scale, diarrhea score, valeric acid, and L. paracasei DNA concentration). No changes were observed in the CB group after the intervention. (4) Conclusions: Whole-grain sorghum beverages demonstrated good nutritional value, and consumption of these beverages, with or without L. paracasei, provided health benefits, including improvements in body composition, Castelli index I scores, and intestinal health, in overweight and obese adults.
Quality Variation of Pork Bellies by Cutting Manner and Quality Grade
Authors: Seong Pil-Nam , Lee Jeong-Ah , Song Dong-Heon , Kim Hyun-Wook , Kim Dong-Gun , Jung Samooel , Hoa Van-Ba
Published: September 2024

In the meat industry, the quality grading system is commonly applied to classify carcasses based on quality and value. Presently, to facilitate consumer convenience, pork bellies are prepared into slices and retailed in supermarkets and butchers. The objective of this study was to assess the effect of quality grade (QG) and retail cutting manner on the quality properties of pork bellies. Thirty-two bellies with different QGs: QG1+, 1, 2, and off-grade (n = 8 each) randomly collected from a commercial slaughterhouse were used. Each belly was cut into 3 portions: A (5–10th rib, cranial edge), B (11–15th rib), and C (without rib, caudal edge) according to the commonly used retail cutting manner. Samples were subjected to chemical composition, quality traits, and aroma analysis. Fat content was highest in QG1+ and lowest in off-grade and distributed at a higher level in portions A and B than in portion C in all QGs (p < 0.05). Off-grade was associated with higher shear force and chewiness values and lower levels of palmitic and stearic acids, regardless of the cutting portion. The bellies used in this study exhibited variations in chemical composition and quality properties not only among the QGs but also across the cutting portions within each QG.
Volatile Flavor Analysis in Yak Meat: Effects of Different Breeds, Feeding Methods, and Parts Using GC-IMS and Multivariate Analyses
Authors: Li Hongqiang , Xi Bin , Lin Shuqin , Tang Defu , Gao Yaqin , Zhao Xiangmin , Liang Jing , Yang Wanyun , Li Jinlu
Published: September 2024

This study investigates the effects of breeds, feeding methods, and parts on the volatile flavor of yak meat. Gas chromatography–ion mobility spectrometry (GC-IMS) and multivariate analysis were used to analyze the volatile organic components (VOCs) in yak meat from various sources. A total of 71 volatile compounds were identified, 53 of which were annotated based on the GC-IMS database. These include 20 alcohols, 16 ketones, 10 aldehydes, four alkenes, one ester, one acid, and one furan. Using VOC fingerprinting and multivariate analysis, yak meats from different sources were distinctly categorized. Breed had the most significant impact on yak meat VOCs, followed by feeding method and then part. Six volatiles with a variable importance in projection value greater than one were identified as potential markers for distinguishing yak meat. This study offers insights into the flavor profile of yak meat from different sources and demonstrates the efficacy of GC-IMS and multivariate analysis in characterizing and discriminating meats.
Dry-Cured Sausages “Salchichón” Manufactured with a Valorized Ingredient from Red Grape Pomace (Var. Tempranillo)
Authors: D’Arrigo Matilde , Petrón María Jesús , Delgado-Adámez Jonathan , García-Parra Jesús Javier , Martín-Mateos María Jesús , Ramírez-Bernabé María Rosario
Published: September 2024

The inclusion of an ingredient made from red grape pomace (RGP) var. Tempranillo was evaluated for the preservation of a traditional dry-cured sausages (salchichón). The pomace was valorized through thermal blanching (103 °C for 1 min) and hydrostatic high-pressure treatment (600 MPa/5 min) before the addition to salchichón. Four formulations of salchichón were evaluated, including a negative control (NC—without red grape pomace or synthetic additives), positive control (PC—with ascorbic acid and nitrites), low level (LL—0.5%), and high level (HL—1%) of RGP. Physicochemical, microbiological, and sensorial effects were analyzed. RGP reduced the final pH of salchichón and favored the growth of lactic acid bacteria at similar levels as PC. The addition of ascorbic acid and nitrites resulted in a final product with a redder and less yellow color than the other formulations. This cured color was not reached with the addition of RGP. However, its inclusion slightly reduced lipid and protein oxidation in salchichón. PC showed high levels of sulfur and terpene levels in a volatile profile, although at a sensory level, only differences in spicy taste were not noticed by panelists. The incorporation of the ingredient could enable the substitution of nitrites with valorized red grape pomace in sausages, although the desirable color achieved with nitrifying salts was not fully attained.
Ultrafiltered Sweet Buttermilk as Additive Replacer in Ice Cream Production
Authors: Ivanova Mihaela , Alinovi Marcello , Dushkova Mariya , Trublet Luca , Rinaldi Massimiliano , Barbanti Davide , Chiavaro Emma , Petkova Zhana , Teneva Olga , Menkov Nikolay
Published: September 2024

Sweet buttermilk, a by-product of butter production, remains highly underutilized despite containing some relevant components (i.e., phospholipids) that may have a high biological value and may exert some positive technological functions. The aim of this study was to investigate the possibility of using ultrafiltered (UF) sweet buttermilk at different volume reduction ratios (3 and 5) to replace emulsifiers and/or stabilizers in the production of a novel clean-label ice cream formulation made with sweet buttermilk-based mixtures. The functional, thermo-rheological, and sensory profile of four types of ice creams was investigated. Increasing the degree of sweet buttermilk concentration positively influenced the overrun values and at the same time improved the ice cream’s resistance to melting. Also, the thermo-rheological profile during melting was influenced by the presence of UF buttermilk. These differences in techno-functional properties were probably partly caused by the different total phospholipids content caused by UF buttermilk. Some sensory properties (i.e., structure, consistency) were positively related to the utilization of UF buttermilk, while aroma and taste were negatively influenced. This study demonstrated that UF buttermilk can be used as an additive replacer in ice cream production because it enhances the structural and rheological properties of the final product.
The Effect of Food Matrix Taken with Probiotics on the Survival of Commercial Probiotics in Simulation of Gastrointestinal Digestion
Authors: Treven Primož , Paveljšek Diana , Bogovič Matijašić Bojana , Mohar Lorbeg Petra
Published: September 2024

The adequate survival of probiotics in the harsh environment of the gastrointestinal (GI) tract plays a crucial role in the expression of their functional properties. The aim of the present study was to evaluate the survival of commercial probiotics during digestion using a standardised INFOGEST 2.0 model extended with three food matrices simulating three scenarios for the consumption of probiotics: on an empty stomach, with juice, or with food (porridge). All eight products matched the bacterial content stated on the label. After simulated digestion, we observed an average decrease in viability of 1.6 log10 colony forming units (CFU) when the product was co-digested with water, a 2.5 log10 CFU decrease in the presence of juice, and a 1.2 log10 CFU decrease in the presence of porridge. The survival rate of the probiotics was statistically higher in the test samples with porridge (91.8%) than in those with juice (79.0%). For two products, the number of lactobacilli and bifidobacteria strains after digestion was less than <3 × 105 CFU, which can be considered insufficient. The present study has shown that the survival of probiotic strains during GI passage depends not only on their ability to withstand these harsh conditions but may also be influenced by the manufacturing process and by the foods consumed together with the probiotics.
Pentacyclic Triterpenoid Content in Cranberry Raw Materials and Products
Authors: Xue Liang , Carreiro Bianca , Mia Md Sagir , Paetau-Robinson Inke , Khoo Christina , Neto Catherine
Published: September 2024

Cranberry fruit extracts have been shown to inhibit expression of pro-inflammatory cytokines in THP-1 cells and reduce colonic tumor burden and tissue inflammation in a mouse model of colitis. These activities are attributed to both the triterpenoid and polyphenol constituents of the fruit. The pentacyclic triterpenoids ursolic acid (UA), oleanolic acid (OA), corosolic acid (CA), maslinic acid (MA), and esters of UA and OA occur in the waxy layer of cranberry peel, and their content in cranberry products is likely to vary with the fruit source and processing methods. UPLC-MS (ultra performance liquid chromatography-mass spectrometry) was applied to determine the four triterpenoid acids and their esters in cranberry products and raw materials. Cranberry pomace, a side stream in juice production, was a rich source at 64,090 µg total triterpenoids/g DW. Cranberry juice beverages ranged from 0.018 to 0.26 µg/g of product, fruit samples ranged from 6542 to 17,070 µg/g DW, and whole berry products contained up to 2665 µg/g DW. Free UA was the most plentiful triterpenoid in all samples. These analyses illustrate the potential value of an underutilized side stream in cranberry juice production and highlight potential benefits of whole fruit products.
Qualitative Traits and Antioxidant Properties of Blood Oranges Are Affected by the Genotype and the Climatic Conditions
Authors: Modica Giulia , Legua Pilar , La Malfa Stefano , Gentile Alessandra , Continella Alberto
Published: September 2024

Blood oranges are increasingly cultivated worldwide as consumers become more aware of the health benefits of their nutraceutical properties and natural antioxidants, specifically polyphenols and anthocyanins. The amounts of these compounds in the fruit mostly depend on the cultivar, rootstock, maturity stage, and environmental conditions. This work focused on the study of the qualitative features of numerous blood orange cultivars grown in three different environments in Spain and Italy. The aim of the work was to investigate the accumulation of primary and secondary metabolites, including bioactive compounds, and to characterize fruit qualitative traits at the time of harvest. Simple sugars were identified and quantified by liquid chromatography and organic acids, polyphenols, and flavonoids by spectrophotometric analysis. The antioxidant potential of the juice was assessed by ABTS, DPPH, and FRAP assays. Cultivation area affected juice color, with Moro and T. Ippolito being the varieties with the highest pigmentation. The cultivation area also determined the pattern of primary and secondary metabolite accumulation in the Tarocco lines. Furthermore, the antioxidant potential was influenced by the diverse environments. Principal Component Analysis highlighted three clusters, two overlapping clusters for the varieties grown in the two Spanish plots and a third clearly separated cluster for the genotypes grown in Italy. This study provides novel knowledge on primary and secondary metabolite accumulation in blood oranges, elucidating the role of genotype and environmental conditions on fruit quality.
Effect of Feed on the Growth Performance, Nutrition Content and Cost of Raising the Field Cricket (Gryllus madagascarensis) as a Sustainable Nutrient Source in Madagascar
Authors: Magara Henlay J. O. , Hugel Sylvain , Fisher Brian L.
Published: September 2024

The field cricket, Gryllus madagascarensis, is a sustainable and nutritious food resource that has the potential to mitigate global malnutrition. Feeds provided to this cricket can influence its growth parameters, nutritional content, and the cost of raising it for food. The current study aimed to evaluate the effects of feeds formulated from weeds, agro-byproducts, and chicken feed (control) on the growth parameters and nutritional content of G. madagascarensis. The formulated feeds included CFB (25.0% protein), CFC (24.5% protein), CFD (24.0% protein), CFE (23.5% protein), CFF (22.5% protein), CFG (21.5% protein), CFH (20.0% protein), CFI (14.5% protein), and CFJ (13.5% protein), and chicken feed (CFA) (28% protein) was used as the control. The formulation of the feeds was based on the acceptability and protein content of the 12 selected weeds and agro-byproducts. Proximate, mineral, and fatty acid analyses were conducted to determine the nutrient content of each feed, as well as the crickets raised on these feeds. The fastest development time was recorded with CFE and CFC. The highest survivorship (98%) was observed in CFG, CFE, and CFC. The highest body mass (1.15 g) and body length (26.80 mm) were observed in crickets fed CFG. By comparison, crickets fed control feed averaged a body mass of 0.81 g and a body length of 23.55 mm. The feed conversion ratio for G. madagascarensis fed CFG, CFE, and CFC was 1.71. Crickets raised on CFH and CFG had the lowest cost of feeding per kg live mass gain. Crickets fed on CFF had the highest quantity of protein (67%), followed by those fed CFG (65% protein); crickets with the lowest protein content (50%) were fed CFJ. Crickets fed on CFG had the highest mineral content. Linoleic acid, oleic acid, and palmitic acid were the major fatty acids. The findings indicate that formulated feeds from weeds and agro-byproducts have great potential to be used as an alternative feed source for crickets for two reasons: their capacity to positively influence the biology and nutrition of the cricket, and they can serve as an inexpensive replacement for chicken feed.
Reply to Kiskinov et al. Comment on “Vatsala et al. Analysis of Sequential Caputo Fractional Differential Equations versus Non-Sequential Caputo Fractional Differential Equations with Applications. Foundations 2022, 2, 1129–1142”
Authors: Vatsala Aghalaya S. , Pageni Govinda , Vijesh V. Anthony
Published: September 2024

In our article [...]
Theoretical Analysis of Viscoelastic Friction System Characteristics of Robotic Arm Brake Based on Fractional Differential Theory
Authors: Ma Wenli , Du Qiaoling , Li Wenhao , Yang Zhenqi
Published: September 2024

In engineering practice, the nonlinear vibration effect can easily lead to chaos in the system, which will not only reduce the performance of the system but also lead to premature fatigue of components, control failure, and increased safety risks. In view of the core position of the robotic arm in modern industry, this study relies on the robotic arm brake system to explore the theoretical basis of integrated viscoelastic materials as a vibration isolation layer. By analyzing the dynamic characteristics of the friction braking system with fractional differential terms, it aims to provide a new perspective for understanding and controlling the chaotic phenomena of a class of nonlinear friction systems. Firstly, we construct a model of a friction system and analyze its dynamic characteristics in detail. The self-excited vibration of the system under disturbance is studied. The relationship between amplitude and frequency is calculated by a nonlinear approximate analytical algorithm, and the accuracy of this relationship is verified by a numerical algorithm. Then, we compare the differences between non-fractional systems and fractional systems. It is found that with the increase in the fractional order term, the vibration amplitude of the system decreases significantly, which helps to reduce the nonlinear characteristics generated by the friction system and narrow the range of unstable solutions. Secondly, we also study the influence of parameter coefficients on the amplitude–frequency characteristics and analyze the local static bifurcation characteristics through singularity theory. Finally, we study the dynamic bifurcation behavior under different parameter perturbations and find that the change in system parameters will lead to the alternation of periodic motion and chaotic motion.
New Event-Triggered Synchronization Criteria for Fractional-Order Complex-Valued Neural Networks with Additive Time-Varying Delays
Authors: Zhang Haiyang , Zhao Yi , Xiong Lianglin , Dai Junzhou , Zhang Yi
Published: September 2024

This paper explores the synchronization control issue for a class of fractional-order Complex-valued Neural Networks (FOCVNNs) with additive time-varying delays (TVDs) utilizing a sampled-data-based event-triggered mechanism (SDBETM). First, an innovative free-matrix-based fractional-order integral inequality (FMBFOII) and an improved fractional-order complex-valued integral inequality (FOCVII) are proposed, which are less conservative than the existing classical fractional-order integral inequality (FOII). Secondly, an SDBETM is inducted to conserve network resources. In addition, a novel Lyapunov–Krasovskii functional (LKF) enriched with additional information regarding the fractional-order derivative, additive TVDs, and triggering instants is constructed. Then, through the integration of the innovative FOCVII, LKF, SDBETM, and other analytical methodologies, we deduce two criteria in the form of linear matrix inequalities (LMIs) to ensure the synchronization of the master–slave FOCVNNs. Finally, numerical simulations are illustrated to confirm the validity of the proposed results.
Inner Multifractal Dynamics in the Jumps of Cryptocurrency and Forex Markets
Authors: Ali Haider , Aftab Muhammad , Aslam Faheem , Ferreira Paulo
Published: September 2024

Jump dynamics in financial markets exhibit significant complexity, often resulting in increased probabilities of subsequent jumps, akin to earthquake aftershocks. This study aims to understand these complexities within a multifractal framework. To do this, we employed the high-frequency intraday data from six major cryptocurrencies (Bitcoin, Ethereum, Litecoin, Dashcoin, EOS, and Ripple) and six major forex markets (Euro, British pound, Canadian dollar, Australian dollar, Swiss franc, and Japanese yen) between 4 August 2019 and 4 October 2023, at 5 min intervals. We began by extracting daily jumps from realized volatility using a MinRV-based approach and then applying Multifractal Detrended Fluctuation Analysis (MFDFA) to those jumps to explore their multifractal characteristics. The results of the MFDFA—especially the fluctuation function, the varying Hurst exponent, and the Renyi exponent—confirm that all of these jump series exhibit significant multifractal properties. However, the range of the Hurst exponent values indicates that Dashcoin has the highest and Litecoin has the lowest multifractal strength. Moreover, all of the jump series show significant persistent behavior and a positive autocorrelation, indicating a higher probability of a positive/negative jump being followed by another positive/negative jump. Additionally, the findings of rolling-window MFDFA with a window length of 250 days reveal persistent behavior most of the time. These findings are useful for market participants, investors, and policymakers in developing portfolio diversification strategies and making important investment decisions, and they could enhance market efficiency and stability.
Toxic Effect of Methyl-Thiophanate on Bombyx mori Based on Physiological and Transcriptomic Analysis
Authors: He Zhen , Fang Yang , Zhang Fengchao , Liu Yang , Wen Xiaoli , Yu Cui , Cheng Xinkai , Li Dechen , Huang Liang , Ai Hui , Wu Fan
Published: September 2024

Background/Objectives: The utilization of methyl-thiophanate (MT) in vegetables and fruits is widespread due to its broad efficiency, yet its potential impact on silkworm growth remains uncertain. This study aims to examine the effects of MT on the growth of silkworms. Specifically, we assessed the weights of fifth-instar larvae that were fed mulberry leaves saturated with three concentrations (2.5, 5, and 10 mg/mL) of MT, as well as the weights of a control group. Methods: TEM was used to show the status of the silkworm midgut after MT supplementation. Oxidative stress was evaluated in the presence of MT. Furthermore, a transcriptomic sequencing experiment was conducted to investigate the mechanism through which the development of silkworms is induced by MT. Results: Our findings indicate that the supplementation of MT hindered larval growth compared to the control group, suggesting a toxic effect of MT on silkworms. The transmission electron microscopy (TEM) results show that MT supplementation induced autophagy in the silkworm midgut. MT was also found to induce oxidative stress in silkworms through the activation of reactive oxygen (ROS), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. Subsequent transcriptomic analysis revealed 1265 significantly differentially expressed genes (DEGs) in response to MT. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these DEGs were associated with antioxidant defense, detoxification processes, lysosome biogenesis, and metabolic pathways. Conclusions: These findings suggest that MT toxicity in silkworm larvae is mediated through the induction of oxidative stress and alterations in metabolism. This study contributes to our understanding of the impacts of MT exposure on silkworms and provides insights into potential pesticides for use in mulberry gardens.
Variation in the Local Grey Mullet Populations (Mugil cephalus) on the Western Pacific Fringe
Authors: Kuo Chien-Hsien , Lee Sin-Che , Du Shin-Yi , Huang Chao-Shen , Lin Hung-Du
Published: September 2024

Background: Understanding population genetic structures is crucial for planning and implementing conservation programmes to preserve species’ adaptive and evolutionary potential and thus ensure their long-term persistence. The grey mullet (Mugil cephalus) is a globally distributed coastal fish. Its populations in waters surrounding Taiwan on the western Pacific fringe are divided into at least two stocks (migratory and residential), but questions remain regarding their genetic divergence and gene flow. Methods and Results: To cast more light on this, allozyme variations at 21 presumptive gene loci of 1217 adult grey mullets from 15 localities in Japan, Taiwan and mainland China, and four gene loci from 1470 juveniles from three localities in Taiwan were used to investigate patterns of genetic variation. The mean expected heterozygosity (He) was 0.128—ranging from 0.031 (Matsu) to 0.442 (Kaoping)—and the mean observed heterozygosity (Ho) was 0.086—ranging from 0.017 (Kaohsiung) to 0.215 (Kaoping). Both AMOVA and the high overall mean FST of 0.252 indicated enormous genetic differentiation among populations and the positive mean value of FIS was 0.328, indicating a deficiency of heterozygotes. PCoA indicated that the samples of M. cephalus could be split into three groups and STRUCTURE analysis showed that all individuals were grouped into three genetic clusters. The results of mutation-drift equilibrium tests did not suggest that the populations experienced any recent genetic bottleneck. The results from all localities in the present investigation showed significant change in the GPI-A genotype frequencies with latitudes—e.g., increases in GPI-A*135/135 homozygote frequencies and GPI-A*100/100 frequencies were highly correlated with latitudinal cline. All migratory populations with the GPI-A genotype were almost exclusively the GPI-A*100/100 homozygote. During the life history of M. cephalus, the GPI-A*100/135 heterozygote frequency significantly decreases with age. Conclusions: Based on these data, we suggest that each GPI-A genotype represents trait combinations of higher fitness in some portions of the environment. Furthermore, the genotypic frequencies change in accordance with life stages, suggesting that selection occurs throughout the life span.
DNA Methylation Participates in Drought Stress Memory and Response to Drought in Medicago ruthenica
Authors: Zi Na , Ren Weibo , Guo Huiqin , Yuan Feng , Liu Yaling , Fry Ellen
Published: September 2024

Background: Drought is currently a global environmental problem, which inhibits plant growth and development and seriously restricts crop yields. Many plants exposed to drought stress can generate stress memory, which provides some advantages for resisting recurrent drought. DNA methylation is a mechanism involved in stress memory formation, and many plants can alter methylation levels to form stress memories; however, it remains unclear whether Medicago ruthenica exhibits drought stress memory, as the epigenetic molecular mechanisms underlying this process have not been described in this species. Methods: We conducted methylome and transcriptome sequencing to identify gene methylation and expression changes in plants with a history of two drought stress exposures. Results: Methylation analysis showed that drought stress resulted in an approximately 4.41% decrease in M. ruthenica genome methylation levels. The highest methylation levels were in CG dinucleotide contexts, followed by CHG contexts, with CHH contexts having the lowest levels. Analysis of associations between methylation and transcript levels showed that most DNA methylation was negatively correlated with gene expression except methylation within CHH motifs in gene promoter regions. Genes were divided into four categories according to the relationship between methylation and gene expression; the up-regulation of hypo-methylated gene expression accounted for the vast majority (692 genes) and included genes encoding factors key for abscisic acid (ABA) and proline synthesis. The hypo-methylation of the promoter and body regions of these two gene groups induced increased gene transcription levels. Conclusions: In conclusion, DNA methylation may contribute to drought stress memory formation and maintenance in M. ruthenica by increasing the transcription levels of genes key for ABA and proline biosynthesis.
Family Occurrence of an m.3303C>T Point Mutation in the MT-TL1 Gene, Which Induces Cardiomyopathy Syndrome with/without Skeletal Muscle Myopathy
Authors: Fałek Olga , Wesół-Kucharska Dorota , Starostecka Ewa , Rokicki Dariusz , Fortecka-Piestrzeniewicz Katarzyna , Kępczyński Łukasz , Piekutowska-Abramczuk Dorota , Ciara Elżbieta , Maroszyńska Iwona
Published: September 2024

This paper discusses the cases of siblings that were born healthy, then diagnosed in their neonatal periods with cardiomyopathy and/or severe metabolic acidosis, which ran progressive courses and contributed to death in infancy. Molecular testing of the children confirmed the presence of an m.3303C>T point mutation in the mitochondrial DNA in the MT-TL1 gene, which was also present in their oligosymptomatic mother and their mother’s sister, an asymptomatic carrier.
Application of the Coastal and Marine Ecological Classification Standard (CMECS) to Create Benthic Geologic Habitat Maps for Portions of Acadia National Park, Maine, USA
Authors: Oakley Bryan , Caccioppoli Brian , LaFrance Bartley Monique , Johnson Catherine , Moen Alexandra , Soulagnet Cameron , Rondeau Genevieve , Rego Connor , King John
Published: September 2024

The Coastal and Marine Ecological Classification Standard (CMECS) was applied to four portions of Acadia National Park, USA, focusing on intertidal rocky and tidal flat habitats. Side-scan sonar coupled with multi-phase echo sounder bathymetry are the primary data sources used to map the seafloor, coupled with underwater video imagery and surface grab samples for grain size and macrofaunal analysis. The CMECS Substrate, Geoform, and Biotic components were effective in describing the study areas. However, integrating the CMECS components to define Biotopes was more challenging due to the limited number of grab samples available and because the dominant species within a given map unit is largely inconsistent. While Biotopes ultimately could not be defined in this study, working within the CMECS framework to create statistically significant biotopes revealed the complexity of these study areas that may otherwise have been overlooked. This study demonstrates the effectiveness of the CMECS classification, including the framework’s ability to be flexible in communicating information.
The Relationships between the Internal Nappe Zone and the Regional Mylonitic Complex in the NE Variscan Sardinia (Italy): Insight from a New Possible Regional Interpretation?
Authors: Elter Franco Marco , Mantovani Federico
Published: September 2024

This study presents an updated interpretation of geological data collected between 1984 and 2022. The area under consideration holds significant regional importance as it is located between the Internal Nappe Zone (INZ) and the Regional Mylonitic Complex (RMC). Re-evaluation of the geological data has highlighted a more intricate structural framework than what is currently documented in the existing literature. This paper aims to illustrate, through structural analysis, that the Posada Valley Shear Zone (PVSZ) does not serve as the transitional boundary between the Inner Nappe Zone and the Regional Mylonitic Complex or High-Grade Metamorphic Complex (HGMC) as traditionally thought. Instead, the authors’ findings indicate that the transition boundary is confined to a shear band with a variable thickness ranging from 10 to 70 m at its widest points. The development of the Posada Valley Shear Zone is characterized by a series of transitions from mylonite I S-C to mylonite II S-C, extending over approximately 5 km. The formation of the Posada Valley Shear Zone is chronologically confined between the development of the East Variscan Shear Zone (EVSZ) and the emplacement of the Late Variscan granites. The differing orientations of Sm and S3 observed in the mylonitic events of the Posada Valley Shear Zone and the Regional Mylonitic Complex, respectively, are likely attributable to an anticlockwise rotation of the shortening directions during the upper Carboniferous period. Furthermore, this study proposes that the Condensed Isogrades Zone (CIZ), despite its unclear formation mechanism, should be recognized as the true transition zone between the Inner Nappe Zone and the Regional Mylonitic Complex or High-Grade Metamorphic Complex. This new interpretation challenges the previously accepted notion of increasing Variscan metamorphic zonation toward the northeast. This conclusion is supported by the identification of the same NE–SW orientation of the D2 tectonic event in both the Old Gneiss Complex (OGC in the Regional Mylonitic Complex) and the lithologies of the Inner Nappe Zone and the Condensed Isogrades Zone. The comprehensive analysis and new insights provided in this paper contribute to a refined understanding of the geological relationships and processes within this region, offering significant implications for future geological studies and interpretations.