bluuebunny/crossref_metadata_2025 huggingface dataset:
An ensemble learning method based on ordinal regression for COVID-19 diagnosis from chest CT
Authors: Guo Xiaodong , Lei Yiming , He Peng , Zeng Wenbing , Yang Ran , Ma Yinjin , Feng Peng , Lyu Qing , Wang Ge , Shan Hongming
Published: October 2021

Coronavirus disease 2019 (COVID-19) has brought huge losses to the world, and it remains a great threat to public health. X-ray computed tomography (CT) plays a central role in the management of COVID-19. Traditional diagnosis with pulmonary CT images is time-consuming and error-prone, which could not meet the need for precise and rapid COVID-19 screening. Nowadays, deep learning (DL) has been successfully applied to CT image analysis, which assists radiologists in workflow scheduling and treatment planning for patients with COVID-19. Traditional methods use cross-entropy as the loss function with a Softmax classifier following a fully-connected layer. Most DL-based classification methods target intraclass relationships in a certain class (early, progressive, severe, or dissipative phases), ignoring the natural order of different phases of the disease progression, i.e., from an early stage and progress to a late stage. To learn both intraclass and interclass relationships among different stages and improve the accuracy of classification, this paper proposes an ensemble learning method based on ordinal regression, which leverages the ordinal information on COVID-19 phases. The proposed method uses multi-binary, neuron stick-breaking (NSB), and soft labels (SL) techniques, and ensembles the ordinal outputs through a median selection. To evaluate our method, we collected 172 confirmed cases. In a 2-fold cross-validation experiment, the accuracy is increased by 22% compared with traditional methods when we use modified ResNet-18 as the backbone. And precision, recall, and F1-score are also improved. The experimental results show that our proposed method achieves a better classification performance than the traditional methods, which helps establish guidelines for the classification of COVID-19 chest CT images.
Nonlinear MHD simulation of core plasma collapse events in Wendelstein 7-X
Authors: Suzuki Yasuhiro , Futatani Shimpei , Geiger Joachim
Published: October 2021

Three-dimensional nonlinear MHD simulations study the core collapse events observed in a stellarator experiment, Wendelstein 7-X. In the low magnetic shear configuration like the Wendelstein 7-X, the rotational transform profile is very sensitive to the toroidal current density. The 3D equilibrium with localized toroidal current density is studied. If the toroidal current density follows locally in the middle of the minor radius, the rotational transform is also changed locally. Sometimes, the magnetic topology changes due to appearing the magnetic island. A full three-dimensional nonlinear MHD code studies the nonlinear behaviors of the MHD instability. It was found that the following sequence. At first, the high-n ballooning-type mode structure appears in the plasma core, and then the mode linearly grows. The high-n ballooning modes nonlinearly couple and saturate. The mode structure changes to the low-n mode. The magnetic field structure becomes strongly stochastic into the plasma core due to the nonlinear coupling in that phase. Finally, the plasma pressure diffuses along the stochastic field lines, and then the core plasma pressure drops. This is a crucial result to interpret the core collapse event by strong nonlinear coupling.
Forced convection heat transfer from a plate stack embedded in a homogeneous porous medium
Authors: Tul Kubra Khadija , McKibbin Robert , Ullah Sana , Khalid Muhammad , Sweatman Winston L
Published: October 2021

The current paper examines some novel and interesting aspects of the mathematical modelling of fluid flow and forced convection heat transfer from a plate stack embedded in a homogeneous porous media. Time-dependent forced convection heat transfer from some cylinders embedded in groundwater are explored numerically using finite difference methods. An algorithm was developed about rectangular heated solid objects with the same size placed in an in-line arrangement in homogeneous porous media. Central and one-sided finite difference formulae are used to discretize the domain. Applying the mathematical model, continuity, momentum, and energy equations are solved by the finite difference methods. Generally, analytical solutions are not possible for such problems, so most of the work is done numerically. The numerical results for the two-dimensional velocity potential and stream function have been calculated and the resulting fluid and temperature contours are represented graphically for various values of the parameters involved (e.g. length of the entrance, the fluid flow rates, coefficient of thermal diffusion and advection in the observed long-time plume behaviour, effect of time and temperature on heat transfer, etc.). It is observed that fluid and heat fluxes and fluid velocity depend upon the size, shape, burial depth, position of heated objects, and positions of the entrance and exit of the homogeneous porous media. Moreover, the hydraulic conductivity of the porous medium affects the pressure drops, fluid velocity as well as the heat transfer rates.
Modernization of the hardware and software complex of the national standards service in the area of pressure measurement technology for the calibration of vacuum gauges and development of a calibration simulator
Authors: Kuvandykov R E , Chernyshenko A A
Published: October 2021

The article provides information about the functions and the algorithm of modernization of the software and hardware complex for automating the calibration process of vacuum gauges, offers software as a simulator for learning the procedure for calibrating vacuum gauges and effective training of testers. It is shown that in the department for national standards in the field of pressure measurement of D. I. Mendeleyev Institute for Metrology VNIIM. The modernization makes it possible to reduce the work intensity of the test procedure, to reduce the influence of the "human factor", to increase the work productivity of the inspector and to reduce the training time for inspectors in the field of vacuum measurements.
Thin film coatings material particles creation by pulsed methods in vacuum
Authors: Panfilov Y V , Kolesnik L L , Gurov A V
Published: October 2021

Thin film materials particles creation pulsed methods such as magnetron sputtering HiPIMS, pulsed laser deposition PLD, vacuum arc pulsed discharge, high-intensity pulsed ion beam impact HIPIB, as well, were described. It was shown that the stream of material, created by means of an explosion action such as ablation, avalanche paired impacts and microsecond electrical disruption as well creates preconditions for nanocrystalline thin film coating manufacture.
Estimation rumen degradable protein of local feeds in dairy cattle using in sacco method
Authors: Rosmalia A , Permana I G , Despal  , Zahera R
Published: October 2021

There is a lack of nutrition information on local feeds protein in Indonesia, especially to determine protein fraction of dairy feed. The objective of this study was to determine rumen degradable protein (RDP) of local feeds in dairy cattle using in sacco method. The local feeds are copra meal, palm kernel meal, coffee husk, tofu waste, soy-sauce waste, brewer waste, and habbatussauda waste. Two ruminal fistulated male Frisian Holstein were used to determine rumen degradable protein using in sacco method. The parameters observed include the estimated kinetic parameters, effective degradability (ED), and RDP. The parameters were evaluated with analysis of variance using SAS University software. The result showed that tofu waste and habbatussauda waste had high potential rapid degradation of CP following by brewer waste, palm kernel waste, soy-sauce waste, copra meal, and coffee husk. Habbatussauda waste had higher RDP followed by brewer waste, tofu waste, copra meal, palm kernel meal, soy-sauce waste, and coffee husk. There was a positive correlation between RDP and crude protein content, and a negative correlation between RDP and crude fiber. It is concluded that local feeds have various characteristics of quality feed protein, which is shown by rumen degradable protein and rumen undegradable protein.
The evaluation of protease enzyme effectivenes in broiler chicken diet containing jack bean seed (Canavalia ensiformis) with different protein level toward internal organ size
Authors: Mahardhika B P , Ridla M , Mutia R , Adli D N
Published: October 2021

This study was conducted with the intention of evaluating the use of the protease enzyme in broiler feed containing jack bean seed (Canavalia ensiformis) with the different levels of protein towards the internal organs of broiler chicken starter. Bird samples used were 24 broilers of the male lohmann strain taken from 120 chickens. The study design used was a factorial completely randomized design (FCRD) with 2 factors treatment and 3 replications (each replication used 2 samples of bird). The first factor was the level of protein (CP 22% and CP 19.5%) and the second factor was the use of enzymes (non-protease and protease). Data were analyzed by using analysis of variance (ANOVA). The result showed that the use of low protein levels in broiler feed containing jack bean seed significantly (P <0.05) increased the percentage of pancreatic weights but could be improved by the use of protease enzymes. Levels of protein treatment did not significantly affect the percentage of heart, liver and bile weights. The use of protease enzymes significantly (P <0.05) decreased the percentage of pancreatic and bile weights but did not significantly affect the percentage of liver and heart weights.
Introduction to a systematic review and meta-analyses in Indonesia nutrition poultry: case study in probiotic
Authors: Adli D N , Sjofjan O , Jayanegara A , Mahardika B P
Published: October 2021

The review conducted to Introduction to a systematic review and meta-analysis in Indonesia nutrition poultry: case study in feed additive. A review on the table showed the origin phase research and introduction in Indonesia (representatives for animal sciences) both meta-analyses and probiotic. The result showed first generation origin of probiotic started on Indonesia the oldest one were from Aspergillus sp and Bacillus sp with meta-analysis started with small ruminant. Meta-analyses origin with author from Indonesia on animal science were started with title Influence of dietary tannin levels on methane production from ruminant livestock: a meta-analysis (Anuraga Jayanegara, 2010). To sum up, the idea help to increase in industry revolution 4.0 were adaptable and applicator.
Mycorrhizal fungi Glomus spp. propagation in zeolite enriched with mycorrhiza helper bacteria for controlling nematode in coffee
Authors: Asyiah I N , Hindersah R , Harni R , Fitriatin B N , Anggraeni W
Published: October 2021

Arbuscular mycorrhizal fungi (AMF) play a role in suppressing the nematode Pratylenchus coffeae. Mycorrhizal helper bacteria (MHB) can increase the effectiveness of AMF to control the diseases. The experimental purpose was to increase the spore population of AMF Glomus spp. in zeolite-based formulation inoculated with liquid consortia of Pseudomonas diminuta and Bacillus subtilis as MHB. The experimental design was a completely random design with six treatments consisted of 106, 107, 108, and 109 CFU/mL MHB liquid inoculants. The control treatments were water and 2% molasses. All treatments were replicated four times. A total of 300 mL/pot Liquid inoculant of MHB have been inoculated a three day before transplanting the maize seedling to the Zeolite inoculated with Glomus spp. in the pot. One month after MHB inoculation, Glomus formulation in Zeolite with different levels of MHB increased the degree of infection. Three months after MHB inoculation, spore content in Zeolite increased. The density of P. diminuta and B. subtilis in zeolite-based mycorrhizal inoculant increased at the end of the experiment. Liquid inoculant MHB contained 108 CFU/mL enhanced spora number fourth times compared to the control. This experiment suggests that P. diminuta and B. subtilis were effective to increase the spore density of AMF inoculant.
Activity test of Bacillus Spp against bacterial wilt (R. solanacearum) on tomatoes by in vitro
Authors: Tuhumury G N C , Hasinu J V , Kesaulya H
Published: October 2021

Ralstonia solanocearum is a pathogenic bacteria that attacks tomatoes and causes wilt disease. Many efforts have been made to control this disease through cultivation, use of chemical pesticides, and development of resistant varieties, but bacterial wilt disease remains a serious problem economically. Nowadays, many biological controls are being developed using microbes. The use of Bacillus spp as an unfriendly microbe is very potential to control because it has pathogenic inhibitory activity. This study aims to obtain bacterial isolates of Bacillus spp which can suppress the development of bacterial wilt disease in tomato plants. The research was conducted in vitro at the Laboratory of Plant Physiology in the Agriculture Faculty, Unpatti. The results showed that Bacillus niabensis strain PT-32-1 and Bacillus subtilis strain SW116b could inhibit Ralstonia solanacearum, wilt disease in tomato plants in vitro.
Edible insects: Alternative protein for sustainable food and nutritional security
Authors: Tuhumury H C D
Published: October 2021

Any food systems applied has to ensure that the food is secure for the people. Nutritional needs are considered integral to the food security concept and sustainability is also important. One of the nutritional needs in food is protein. Proteins mostly obtained from animal-based sources such as meat and fish, plant-based proteins, particularly legumes. However, the production of meat, more importantly ruminant meat is more and more argued for some issues including environment, food safety, and animal welfare. In order to ensure sustainable protein production, the dietary change would be to use alternative protein sources such as insects. In general, insects have high protein content and excellent production efficiency compared with other conventional protein food groups. It is necessarily important to develop the use of insects in various forms, including pastes or powders, concentrates or isolates which can be used further as ingredients or fortified agents in new food product formulations. This paper therefore explores the feature of edible insects as sustainable and viable food source that can contribute to food security including, types of edible insects as food and their nutritional compositions; and processing of edible insects. In conclusion, insects can contribute to sustainable food and nutritional security and be a part of the solution to protein shortages. Insect protein concentrates could potentially be an alternative protein source in food formulation.
Farmers empowerment level analysis in farming during the Covid-19 pandemic and its impact on farm income
Authors: Damanik I P N , Tahitu M E , Turukay M , Adam F P
Published: October 2021

This study aims to analyze the level of farmer empowerment in carrying out farming activities as the main livelihood and its impact on farm income during the Covid-19 pandemic. The research was conducted online by distributing questionnaires in the form of google form in various affordable areas. There were 113 responses obtained by returning the google form on time. The three regions that sent the most responses were farmers in Ambon City, West Seram Regency, and Central Maluku Regency. After analyzing using qualitative descriptive analysis, the results showed that the level of farmer empowerment during the Covid-19 pandemic decreased, especially in terms of marketing of crop products for fruit and vegetable commodities, while for food commodities such as rice tended to be stable. This has an impact on farm productivity and income. The main factor causing a decrease in the level of farmer empowerment is the decreasing purchasing power of the community during the Covid pandemic19; Accessibility of farmers to markets is difficult, there are even times when farmers cannot go to the market due to large-scale social restrictions, the frequency of presence of collectors is reduced, access to plant production facilities is hampered unlike before the Covid pandemic19. This condition requires serious attention from the government so that farmers do not experience difficulties in marketing agricultural products and can maintain the stability of farm income. The solution at the farmer level is to form farmer groups so that the marketing of farm products and the fulfillment of plant production facilities can be done through farmer groups.
Response of soybean (Glycine max L.) that was applied by various liquid organic fertilizer in climate change at acid soil
Authors: Jamilah  , Rapialdi  , Ernita M
Published: October 2021

Acidic soils are formed from rapid climate change. Acid soils have problems in it, among others; high levels of Al, Fe, and Mn and the unavailability of elements P and N for staple crops. Indonesia imports almost 70% of its domestic requirement. The purpose of this study was to determine the response of soybean plants that were given various types of liquid organic fertilizer (LOF) to climate change in acid soils. Experiments have been carried out in acid soil in the Kuranji area of Padang City. Materials needed include; Mutiara-1 seed. The lime and LOF trials were arranged in a completely randomized design (CRD), with 9 treatments namely; without calcification and without LOF (A); give lime equivalent to 1 x Exchangeable Al and without LOF (Control) (B); Crocober (C); Crocoberma (D); Crocoberderma (E); Titocroco (F); Titocrocoma (G); (H); Trichoderma harzianum (I). The observational data were analyzed statistically by using an F level of 5% significance level if the treatment had a significant effect, it was followed by a LSD test of 5% significance level. Observation parameters include; agronomic, pH soil. Experimental results established that given of dolomite lime can increase soil pH, Titocrocoma LOF accompanied by liming gives the best effect on soybean varieties Mutiara-1 at vegetative stadium, produces 3.45 Mg ha−1 forage dry weight equivalent to107,64 kg N ha−1 or 618,93 kg ha−1 crude protein.
The effect of organic fertilizers on growth several varieties of soybeans
Authors: Rizwan M , Dalimunthe M , Pasaribu I A , Satriawan H
Published: October 2021

This study aims to analyse the effect of three types of organic fertilizers on the vegetative growth several varieties of soybeans. This study used a factorial randomized block design with two treatment factors, namely organic fertilizers and varieties. The first factor is: organic fertilizer from 3 levels, namely: K1 (1.5 kg of compost), K2 (1.5 kg of goat manure), and K3 (1.5 kg of chicken manure). The second factor is: soybean plant varieties consist of 4 levels, namely: V1 (Dena 1), V2 (Devon 1), V3 (Anjasmoro) and V4 (Derap 1). The parameters observed were plant height, number of branches, number of leaves, amount of chlorophyll, leaf area, and flowering age. The results of our research is organic fertilizers have a significant effect and are able to increase the growth of soybean plants except for leaf area and flowering age. Types of varieties had a significant effect and were able to increase plant height, number of branches, leaf area, and flowering age, but had no significant effect on the parameters of the number of leaves and the amount of chlorophyll. Moreover, there isn't interaction effect between the two treatments for all parameters.
Test for the antibacterial inhibition of kaffir lime leaf (Citrus hysteric D.C) extract against pathogen bacteria in improving food safety
Authors: Nendissa S J , Nendissa D M
Published: October 2021

Kafir lime leaf (Citrus hystrix) is a plant from the citrus tribe that has long been known by community as flavor ingredient. To support its use and increase its application in supporting food safety, a test the inhibition of on kaffir lime leaf extract against pathogenic bacteria, namely Gram Negative Bacteria (Escherichia coli, Salmonella typhimurium) and Gram Positive bacteria (Staphylococcus aereus, P. aeroginosa). Making kaffir lime leaf extract (Citrus hystrix) was done by weighing 150g of lime leaf powder, then immersing in 96% ethanol solution and leaving for + 3 days. Kaffir lime leaf extract was dissolved with sterile distilled aquades to obtain a concentration of 5%, 10% and 15%. The antibacterial activity of kaffir lime leaf extract was tested by diffusion method using disc paper to determine of the bacterial growth inhibition area. The results showed that kaffir lime extract had antibacterial activity inhibition of 12,78 mm of S. aereus, 9 mm of E.coli, 7,12 mm of S. typhimurium and 9,3 mm of P. aeroginosa. Kaffir lime leaf extract has inhibition effectiveness for gram positive bacteria Staphylococcus aereus and gram negative bacteria E. coli, Salmonella typhimurium, P. aeroginosa. Thus, kaffir lime leaf extract can be used as a decontaminant agait theses 4 type of bacteria, especially Staphylococcus aereus which has a strong inhibitory power, so it can maintain quality and increase the safety of mead based foods
Evaluation of effectiveness various seed processing combination technique to produce True Shallot Seed (TSS) with good quality
Authors: Waluyo N , Rahayu A , Rosliani R , Wikan T , Gaswanto R
Published: October 2021

The seed processing technique is essential to maintain quality and suppress seed deterioration rate as long as processing time. This research aims to evaluate various seed processing combination techniques to produce TSS with good quality. The study was conducted at Indonesian Vegetables Research Institute, Lembang (1,250 m sal) from March until December 2018. The research used a Randomized Complete Block Design (RCBD) with three replications. The treatment consisted of 12 combinations of seed processing, including the technique of drying, capsule breaking, and sorting. The research result showed that the best treatment was a combination technique with umbels drying in the room at RH 50 % and 30-35°C for 72 hours, breaking capsule by hand manually, and seed sorting by winnower followed by hand manually. The produced seed quality in this treatment showed the seed germination was 75%, the moisture content was 7.5%, and the physical purity was 99.9%. This research implies that the availability of TSS processing technology can be carried out by massal, but still can produce good seed quality.
In vitro propagation of six selected sugarcane mutant clones through leaf explants
Authors: Purnamaningsih R , Sukmadjaja D , Suhesti S , Rahayu S
Published: October 2021

Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.
Performance of native chicken fed on ration containing fermented sago waste
Authors: Ralahalu T N , Latupeirissa CH C E , Hehanussa S CH H , Fredriksz S.
Published: October 2021

The use of fermented sago waste (9 days incubation time) in the ration of native chicken is studied. 100 chicks of 7 days old native chicken were used. The experiment is being conducted in a completely randomized design in which the animals were divided into four treatments of ration. Each treatment was replicated five times. The experimental rations are: R0 (0% fermented sago waste), R1 (5% fermented sago waste), R2 (10% fermented sago waste) and R3 (15% fermented sago waste). Parameters measured were feed intake, feed conversion, body weight gain and carcass percentage. It appeared from this experiment that feeds intake were similar between birds. In grower birds, the use of fermented sago waste in the rations had no significant effect on feed intake. In both, starter and grower birds body weight gain was higher for birds consuming control ration than for those consuming ration with fermented sago waste. The use of 5 up to 15 percent fermented sago waste in the rations significantly affects feed conversion and carcass percentage in both, starter and grower birds.
Identification of endophytic and rhizosphere bacteria in rice (Oryza sativa L.) in the experimental field at Payakumbuh State Agriculture Polytechnic, West Sumatra, Indonesia
Authors: Sondang Y , Siregar R , Anty K
Published: October 2021

The decrease of soil fertility and fewer soil microorganisms will lower crop production, particularly rice, thus threatening the national food security program. This study is (a) to isolate and identify the bacteria in the endophytic and rhizosphere of rice plants (b) to study the bacteria from the endophytic and rhizosphere of rice plants which potentially stimulate plant growth. The experiment was carried out at the Laboratory of Food Crop Cultivation at Payakumbuh State Agriculture Polytechnic, Limapuluh Kota Regency, West Sumatra for four months. The sampling method was carried out by random sampling at rice planting in the Payakumbuh State Agriculture Polytechnic Experimental Field. Endophytic bacteria were taken from the root tissue of rice plants, and rhizosphere bacteria were taken from a layer of soil around rice roots. Isolation of bacteria was carried out by using the pour plate and scratchplate methods. Four bacteria were identified using the 16S rRNA sequencing method. The identification results showed that in the rice root tissue found the bacteria Chromobacterium rhizoryzae and Brevibacillus brevis. In the rice rhizosphere, Bacillus pseudomycoides and Bacillus thuringiensis are found. Bacteria are dominated by the Bacillus genera which can stimulate plant growth.
The physicochemical characteristics of gandaria (Bouea macrophylla) leather with sugar concentration treatment
Authors: Lawalata V N
Published: October 2021

Gandaria leather is a kind of fruit leather processed by gandaria fruit in the form of thin sheets with a thickness of 2-3 mm and has a specific consistency and taste according to gandaria fruit. The purpose of this study was to determine the effect of added sugar on the physicochemical characteristics of gandaria leather. This study used a single-factor completely randomized design, with 0%, 5%, 10%, and 15% sugar concentrations. The results showed that the sugar concentration had a very significant effect on the parameters of water content, total sugar, total acid, vitamin C, and water activity (aw), and had a significant effect on the tensile strength of gandaria leather. The physicochemical characteristics of gandaria leather from various sugar concentration treatments are as follows: water content (14.05% -20.13%), total sugar (80.56% - 84.90%), total acid (3.10 - 12.37 mg/100g), vitamin C (59.44 - 78.48 mg/100g), water activity (0.48 - 0.62), and tensile strength (0.20-0.37 N). The resulting gandaria leather qualifies as good fruit leather, and the treatment with a 10% sugar concentration is recommended for gandaria leather which has the potential to be developed and commercialized.
The utilization of agro-industrial waste for soil amendment and liquid biofertilizer mixed bacterial antagonist in eggplant production
Authors: Boonraeng S , Punyoyai N
Published: October 2021

This research aimed to improve soil fertility with soil amendment, prepared from agro-industrial waste, and application of antagonistic bacteria for controlling bacterial wilt disease. The experiments were tested on sandy-to-sandy loam soil with low organic matter and nutrients for planting eggplants. Besides the soil improvement with spent mushroom waste and mango peel mixed kernel, the results showed that soil pH, soil organic matter, nitrogen, phosphorus, and potassium increased as available levels. Also, two species of bacterial antagonist named Bacillus subtilis and B. amyloliquifaciens were cultured and mixed in liquid biofertilizer. It was spiked for soil preparation before planting and during crop growth every week, which was affected by eggplant survival from bacterial wilt by 100% and 97% in plot and field-tested, respectively. The results from testing on sandy loam with the use of liquid biofertilizer three times/week showed that Ralstonia solanacearum in soil suppressed to the reduction of 1,000-10,000 times. The yield increased by 25.9%. The optimum harvesting time was 12-15 days of fruit growth with the antioxidant activity. Finally, this study has excellent potential to be extended for farmers who organically grown.
Moringa oleifera milk powder as a supplementary food for malnutrition children (SUSUKE)
Authors: Djoko S W , Kembauw E , Kapelle I B D
Published: October 2021

Moringa oleifera are known throughout the world as a nutrition plants, WHO has introduced moringa as an alternative food to overcome malnutrition. In Africa and Asia Moringa leaves are recommended as a nutrient-rich supplements for nursing mothers and children during infancy phase. Moluccas is one of the top 5 regions with quite high rates of malnutrition, while rich in natural products, the lack of knowledge and poor processing method results in nutrients unable to be properly absorbed. Moringa milk-powder can be the answers to malnutrition problems. Methods: This Study used a Nested case-control design, subject were all children aged 1 month-12 years who were diagnosed with malnutrition using WHO and CDC chart during reseach. Result: Sample size was 60 children’s 30 subjects are using malnutrition treatment and moringa milk powder and 30 subjects are using only malnutrition treatment. Conclusion: Moringa milk powder have a benefit for malnutrition children.
Clove processing as a source of increasing business income in Ambon City
Authors: Kembauw E , Mahulette A S , Kakisina A P , Tuhumury M T F , Umanailo M C B , Kembauw M G I
Published: October 2021

Clove is one of the important plantation commodities in Maluku, especially in Ambon City. Clove processing diversification will provide opportunities to increase business income. This study aims to determine the factors that support the diversification of clove processing on the income of entrepreneurs in the city of Ambon. The method used was purposive sampling. The results showed that the diversification of clove processing gave a large enough contribution to income, but the percentage contribution of processing diversification was not visible because the income outside of processing was relatively large. The results showed that the greater the number of species produced, the greater the capital. Based on the analysis, it is known that the level of raw materials available to entrepreneurs to support diversification activities.
A simple discrete-element model for numerical studying the dynamic thermal response of granular materials
Authors: Wu Ze-Xiang , Yang Lei , Wang Zhe , Zhuang Ying-Chun , Tu Dong-Mei
Published: October 2021

This paper aims to investigate the influence of periodicity temperature change on the properties of dry granular materials in macroscopic and microscopic. A series of cyclic thermal consolidation tests have been carried out based on the discrete element method (DEM) that incorporate particles’ volumetric thermal expansion coefficient. The simulation of the direct shear test was carried out on the samples after thermal cycling. Results showed that thermally-induced volumetric strain accumulation of the specimen can be calculated by the DEM model, based on the two-dimensional particle flow code (PFC2D) software. The lateral pressure degraded concomitantly thanks to decreases in particles’ horizontal contact during periodic thermal cycling. In addition, the shear dilatancy level decreases during the shearing process with the number of thermal cycles. Both the size and anisotropy of the normal contact force and contact number and the force chain are affected by the temperature cycle. Finally, the results of this paper have a certain reference for the engineering practice, such as thermal piles or others, when granular materials are subjected to thermal cycling.
Integrated approach in treatment of solid olive residue and olive wastewater
Authors: Altalhi Tariq , El-moemen A Abd , Ibrahim Mohamed M , Mezni Amine , Alsohaimi Ibrahim Hotan , Mahmoud M H H , Kumeria Tushar , Mersal Gaber A M , Mostafa Nasser Y
Published: October 2021

Generally olive oil generated two forms of waste by-products: solid olive residue (SOR) and olive wastewater (OWW). In the present study a promising solution is given to treat both SOR and OWW waste by-products. The first process consists of converting the solid olive residue to activated carbon using pyrolysis process at 600 °C, followed by steam activation procedure at three different temperatures. The attained activated carbon was examined by different experimental techniques such as FTIR, SEM, BET and iodine number. The surface area was increased by increasing stream activation temperature (reach 1020 m2 g−1 BET). However, the steam activation at 700 °C was found to be ideal for environmental and economic performance. On the other hand, the activated carbon at 700 °C displayed high removal capacity of both polyphenolic compounds and COD from olive wastewater. In fact, after 2 h of treatment, 95.5% of COD and 84.2% of polyphenolic compounds were completely removed.
A systematic review of life cycle assessment of hydrogen for road transport use
Authors: Rinawati Dyah Ika , Keeley Alexander Ryota , Takeda Shutaro , Managi Shunsuke
Published: October 2021

This study conducted a systematic literature review of the technical aspects and methodological choices in life cycle assessment (LCA) studies of the use of hydrogen for road transport. More than 70 scientific papers published during 2000–2021 were reviewed, in which more than 350 case studies of the use of hydrogen in the automotive sector were found. Only some studies used hybrid LCA and energetic input–output LCA, whereas most studies addressed attributional process-based LCA. A categorization based on the life cycle scope distinguished case studies that addressed the well-to-tank (WTT), well-to-wheel (WTW), and complete life cycle approaches. Furthermore, based on the hydrogen production process, these case studies were classified into four categories: thermochemical, electrochemical, thermal–electrochemical, and biochemical. Moreover, based on the hydrogen production site, the case studies were classified as centralized, on-site, and on-board. The fuel cell vehicle passenger car was the most commonly used vehicle. The functional unit for the WTT studies was mostly mass or energy, and vehicle distance for the WTW and complete life cycle studies. Global warming potential (GWP) and energy consumption were the most influential categories. Apart from the Greenhouse Gases, Regulated Emissions, and Energy Use in Transportation model and the Intergovernmental Panel on Climate Change for assessment of the GWP, the Centrum voor Milieukunde Leiden method was most widely used in other impact categories. Most of the articles under review were comparative LCA studies on different hydrogen pathways and powertrains. The findings provide baseline data not only for large-scale applications, but also for improving the efficiency of hydrogen use in road transport.
Levels of Parental Drinking in the Presence of Children: An Exploration of Attitudinal Correlates
Authors: Bowden Jacqueline A , Delfabbro Paul , Room Robin , Miller Caroline L , Wilson Carlene
Published: October 2021

Aims

This study aimed to examine perceived social norms, the effect of parental drinking on these norms, alcohol use in front of children, and how norms and consumption vary based on child age and gender of the parent.

Methods

A cross-sectional online panel survey was undertaken with n = 1000 Australian adults (including 670 parents) aged 18–59 years. The survey assessed: alcohol consumption in front of children; normative attitudes towards drinking in the presence of children; and perceived social norms.

Results

Overall, 33.9% of parents reported drinking a glass of alcohol each day or a couple of times a week, 18.2% reported getting slightly drunk and 7.8% indicated getting visibly drunk each day or a couple of times a week with their children present. In total, 37.5% reported drinking in front of their children at least weekly. Fathers were more likely to drink in front of children than mothers. Most parents deemed drinking small amounts of alcohol in front of children as acceptable but did not accept drunkenness. Respondents were less concerned about a father drinking one or two drinks in front of their children than a mother. Social expectations were not related to child age, but norms related to others’ perceived behaviour were.

Conclusions

Many parents, particularly fathers consume alcohol in front of their children. There is a need to target health promotion strategies to adults and parents consuming in excess of health guidelines, and to the many parents who are consuming alcohol at higher levels in front of their children.
Neuropsychological Screening in Pediatric Multidisciplinary Clinics: Group Characteristics and Predictive Utility
Authors: Wolfe Kelly R , Hutaff-Lee Christa , Wilkening Greta
Published: October 2021

Objective

To evaluate the feasibility of administering caregiver-report neuropsychological screening measures in pediatric multidisciplinary clinics (MDCs) and investigate predictive utility for cognitive and academic difficulties on follow-up testing.

Method

The Pediatric Perceived Cognitive Functioning (Peds PCF) and Colorado Learning Difficulties Questionnaire (CLDQ) were administered to caregivers of youth with history of brain tumor (BT), non-central nervous system (CNS) cancer, or Fontan circulation as part of routine neuropsychological consultation in MDCs from 2017 to 2019. We examined cross-sectional differences on screening measures, and relationships between screening and intellectual and academic test performance in a subset who presented for follow-up neuropsychological evaluation. Receiver operating characteristic (ROC) curve analysis was used to identify suggested cut scores on screening measures for predicting who would most benefit from further evaluation.

Results

Screening was completed with 192 families. Children with history of non-CNS cancer (n = 29) had fewer parent-reported cognitive and academic concerns compared with either BT (n = 88) or Fontan (n = 75) groups. Peds PCF and CLDQ scores were related to intellectual and academic test performance in the group presenting for neuropsychological evaluation (n = 68). ROC curve analysis identified cut scores across screening measures with maximal sensitivity and specificity for detecting neuropsychological difficulties.

Conclusions

It is feasible to utilize parent rating measures during neuropsychological consultation in pediatric MDCs. The Peds PCF and CLDQ demonstrated sensitivity to intellectual and academic difficulties in children with significant medical histories. Screening may be a helpful tool for pediatric neuropsychologists and other professionals during MDC consultation to inform clinical management.
Peakhood: individual site context extraction for CLIP-seq peak regions
Authors: Uhl Michael , Rabsch Dominik , Eggenhofer Florian , Backofen Rolf
Published: October 2021

Motivation

CLIP-seq is by far the most widely used method to determine transcriptome-wide binding sites of RNA-binding proteins (RBPs). The binding site locations are identified from CLIP-seq read data by tools termed peak callers. Many RBPs bind to a spliced RNA (i.e. transcript) context, but all currently available peak callers only consider and report the genomic context. To accurately model protein binding behavior, a tool is needed for the individual context assignment to CLIP-seq peak regions.

Results

Here we present Peakhood, the first tool that utilizes CLIP-seq peak regions identified by peak callers, in tandem with CLIP-seq read information and genomic annotations, to determine which context applies, individually for each peak region. For sites assigned to transcript context, it further determines the most likely splice variant, and merges results for any number of datasets to obtain a comprehensive collection of transcript context binding sites.

Availability and implementation

Peakhood is freely available under MIT license at: https://github.com/BackofenLab/Peakhood.

Supplementary information

Supplementary data are available at Bioinformatics online.
spatialTIME and iTIME: R package and Shiny application for visualization and analysis of immunofluorescence data
Authors: Creed Jordan H , Wilson Christopher M , Soupir Alex C , Colin-Leitzinger Christelle M , Kimmel Gregory J , Ospina Oscar E , Chakiryan Nicholas H , Markowitz Joseph , Peres Lauren C , Coghill Anna , Fridley Brooke L
Published: October 2021

Multiplex immunofluorescence (mIF) staining combined with quantitative digital image analysis is a novel and increasingly used technique that allows for the characterization of the tumor immune microenvironment (TIME). Generally, mIF data is used to examine the abundance of immune cells in the TIME; however, this does not capture spatial patterns of immune cells throughout the TIME, a metric increasingly recognized as important for prognosis. To address this gap, we developed an R package spatialTIME that enables spatial analysis of mIF data, as well as the iTIME web application that provides a robust but simplified user interface for describing both abundance and spatial architecture of the TIME. The spatialTIME package calculates univariate and bivariate spatial statistics (e.g. Ripley’s K, Besag’s L, Macron’s M and G or nearest neighbor distance) and creates publication quality plots for spatial organization of the cells in each tissue sample. The iTIME web application allows users to statistically compare the abundance measures with patient clinical features along with visualization of the TIME for one tissue sample at a time.

Availability and implementation

spatialTIME is implemented in R and can be downloaded from GitHub (https://github.com/FridleyLab/spatialTIME) or CRAN. An extensive vignette for using spatialTIME can also be found at https://cran.r-project.org/web/packages/spatialTIME/index.html. iTIME is implemented within a R Shiny application and can be accessed online (http://itime.moffitt.org/), with code available on GitHub (https://github.com/FridleyLab/iTIME).

Supplementary information

Supplementary data are available at Bioinformatics online.
Topical antibiotic prophylaxis for surgical wound infections in clean and clean-contaminated surgery: a systematic review and meta-analysis
Authors: Chen Po-Jung , Hua Yi-Ming , Toh Han Siong , Lee Mei-Chuan
Published: October 2021

Background

Topical antibiotics are widely prescribed as prophylaxis for surgical site infection (SSI). Despite giving high drug concentrations at local wound sites, their efficacy remains controversial. This study is a systematic review and meta-analysis designed to compare the efficacy and safety of topical antibiotics with non-antibiotic agents in preventing SSI.

Methods

Randomized controlled trials (RCTs) comparing topical antibiotics in patients with clean and clean-contaminated postsurgical wounds were included. Relevant trials published before 30 September 2020, were searched in the PubMed, Embase, and Cochrane databases, without language restrictions. The primary outcome was the incidence of SSIs, presented as the event rate. The secondary outcome was the incidence of contact dermatitis (safety outcome). Data were synthesized using the random-effects model, with the results expressed as risk ratio (RR) with 95 per cent confidence intervals (c.i.).

Results

Thirteen RCTs were included. The incidence of SSIs and contact dermatitis showed no significant difference between topical antibiotics and non-antibiotic agents (RR 0.89, 95 per cent c.i. 0.59 to 1.32 (P = 0.56, I2 = 48 per cent); and RR 2.79, 95 per cent c.i. 0.51 to 15.19 (P = 0.24, I2 = 0 per cent), respectively). In the subgroup analyses, a reduction in SSIs was also not observed in dermatological (RR 0.77, 95 per cent c.i. 0.39 to 1.55; P = 0.46, I2 = 65 per cent), ocular (RR 0.08, 95 per cent c.i. 0.00 to 1.52; P = 0.09), spinal (RR 1.34, 95 per cent c.i. 0.65 to 2.77; P = 0.43, I2 = 0 per cent), orthopaedic (RR 0.69, 95 per cent c.i. 0.37 to 1.29; P = 0.25, I2 = 0 per cent), or cardiothoracic surgeries (RR 1.60, 95 per cent c.i. 0.79 to 3.25; P = 0.19).

Conclusion

Given the current evidence, the routine application of topical antibiotics to surgical wounds did not reduce the incidence of SSI. Further trials are needed to assess their effectiveness in high-risk surgeries or in selected patient groups.
Plasma neutrophil gelatinase-associated lipocalin and kidney graft outcome
Authors: Kremer Daan , Post Adrian , Gomes-Neto António W , Groothof Dion , Kunutsor Setor K , Nilsen Tom , Hidden Clara , Sundrehagen Erling , Eisenga Michele F , Navis Gerjan , Bakker Stephan J L ,   TransplantLines Investigators
Published: October 2021

Background

Plasma neutrophil gelatinase-associated lipocalin (pNGAL) has been investigated extensively in acute kidney injury. This study investigated its pathophysiological significance and utility as marker for graft failure and mortality in stable kidney transplant recipients (KTR).

Methods

Baseline pNGAL was measured in 698 KTR (58% male, age 53 ± 13 years, estimated glomerular filtration rate 52.4 ± 20.4 mL/min/1.73 m2) at median 5.4 (interquartile range 1.8–12.0) years after transplantation, enrolled in the prospective TransplantLines Food and Nutrition Biobank and Cohort Study.

Results

pNGAL concentrations were higher in males, younger patients, patients with a deceased-donor kidney and higher serum creatinine. Independent of these, pNGAL was positively associated with urinary protein excretion, systemic inflammation parameters and calcineurin inhibitor use. During median follow-up of 5.3 (4.5–6.0) years, death-censored graft failure rates were 3.9%, 7.3% and 25.0% across increasing tertiles of pNGAL (Plog-rank < 0.001). Cox-regression analyses showed no independent associations of pNGAL with mortality, but strong associations with graft failure (hazard ratio, per doubling 4.16; 95% confidence interval 3.03–5.71; P < 0.001), which remained independent of adjustment for confounders. These associations were present only in patients with pre-existent proteinuria and poor kidney function.

Conclusions

pNGAL is associated with parameters of kidney graft damage and with graft failure. The latter association is particularly present in KTR with pre-existent poor kidney function and proteinuria.

Trial Registration:  ClinicalTrials.gov NCT02811835.