bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an Aotearoa New Zealand adapted Mediterranean dietary pattern and Kai/food basket for the He Rourou Whai Painga randomised controlled trial
Authors: Worthington Anna , Liu Eva , Foster Meika , Wright Summer Rangimaarie , Lithander Fiona E. , Wall Clare , Roy Rajshri , Parry-Strong Amber , Krebs Jeremy , Braakhuis Andrea
Published: July 2024

Background

Following a Mediterranean diet (MedDiet) is associated with a lower risk of cardiovascular disease. He Rourou Whai Painga is a dietary intervention trial with behaviour change support that seeks to determine whether a MedDiet pattern can provide equivalent benefits in Aotearoa New Zealand (NZ), a country where cardiovascular disease is a leading cause of death. To do this, the MedDiet needs to be adapted in an acceptable way for NZ, with consideration of the Māori (indigenous) population.

Methods

The MedDiet was defined using existing MedDiet scoring tools and adapted to the NZ context using local guidelines. The resulting NZ MedDiet pattern was used to develop a kai/food basket, including products from industry partners, for participants in He Rourou Whai Painga. Criteria set for the kai/food basket included providing up to 75% of energy requirements and falling within the Australia/NZ Acceptable Macronutrient Distribution Range to reduce risk of chronic disease. Māori researchers on the team provided support to ensure Mātauranga Māori (Māori knowledge and values) was upheld through this process.

Results

The NZ MedDiet pattern criteria was similar to the identified MedDiet scoring tools, with differences in recommendations for dairy, red meat, alcohol and olive oil. The resulting kai/food baskets were estimated to provide on average 73.5% of energy requirements for households, with 36% from fat, 8.6% from saturated fat, 17% protein, and 42% carbohydrate. Forty-two industry partners, including 3 Māori businesses, agreed to provide 22 types of food products towards the total.

Conclusion

Small, feasible changes to the MedDiet can be made to align with the NZ guidelines and food environment. However, this eating pattern still differs from what the population, particularly Māori, are currently consuming. Continued partnership with Māori and additional behavioural support is important to facilitate adherence to this dietary pattern within He Rourou Whai Painga.

Trial registration: https://www.anzctr.org.au/Default.aspx, identifier ACTRN12622000906752 and https://www.isrctn.com/, identifier ISRCTN89011056.
Alternative splicing regulation and its therapeutic potential in bladder cancer
Authors: Li Lina , Jin Ting , Hu Liang , Ding Jin
Published: July 2024

Bladder cancer is one of the leading causes of mortality globally. The development of bladder cancer is closely associated with alternative splicing, which regulates human gene expression and enhances the diversity of functional proteins. Alternative splicing is a distinctive feature of bladder cancer, and as such, it may hold promise as a therapeutic target. This review aims to comprehensively discuss the current knowledge of alternative splicing in the context of bladder cancer. We review the process of alternative splicing and its regulation in bladder cancer. Moreover, we emphasize the significance of abnormal alternative splicing and splicing factor irregularities during bladder cancer progression. Finally, we explore the impact of alternative splicing on bladder cancer drug resistance and the potential of alternative splicing as a therapeutic target.
Dexamethasone-sparing strategies in anthracycline and cyclophosphamide-based chemotherapy with a focus on 5-HT3 receptor antagonists: a network meta-analysis
Authors: Watanabe Daichi , Iihara Hirotoshi , Kobayashi Ryo , Fujii Hironori , Mori Ryutaro , Kumada Keisuke , Shimizu Masahito , Futamura Manabu , Suzuki Akio
Published: July 2024

Background

The effectiveness of a dexamethasone-sparing strategy in the treatment of breast cancer with anthracycline-cyclophosphamide therapy when combined with first-generation 5-HT3 receptor antagonists (RAs) and neurokinin-1 RAs is unclear. This is attributable to a lack of evidence from direct comparison of multiple doses of DEX to a single dose of DEX in combination with first-generation 5-HT3 RAs in anthracycline-cyclophosphamide therapy. Our goal was to clarify the impact of dexamethasone-sparing strategies that involve both first-generation 5-HT3 RAs and palonosetron when combined with neurokinin-1 RAs, using a network meta-analysis.

Materials and methods

A literature search was conducted on PubMed/Medline for articles published up to July 4, 2023. We included randomized controlled trials which assessed the efficacy of antiemetic regimens which combined 5-HT3 RAs and dexamethasone, with or without neurokinin-1 RAs, for the initial dose in anthracycline-cyclophosphamide therapy for patients with breast cancer. The primary outcome was the proportion of patients achieving a complete response during the delayed phase (CR-DP).

Results

The difference in the proportion of patients achieving CR-DP between multiple and single doses of dexamethasone was 0.1% (95%CI: -12.4 to 12.5) with palonosetron and neurokinin-1 RAs, compared to 5.3% (95%CI: -13.4 to 23.0) with a single dose of a first-generation 5-HT3 receptor antagonist. Additionally, the difference was 12.7% (95% CI: -2.8 to 28.2) when comparing palonosetron against first-generation 5-HT3 RAs in combination with a single dose of dexamethasone and neurokinin-1 RAs.

Conclusion

Palonosetron is recommended rather than a single dose of first-generation 5-HT3 RAs in dexamethasone-sparing strategies for anthracycline-cyclophosphamide therapy.
Congenital absence of the gallbladder in a child: a case report
Authors: Wei Xiao , You Liying , Liu Chun , Xu Xu
Published: July 2024

Background

Congenital absence of the gallbladder (CAGB) is an exceedingly rare embryological anomaly of the biliary system, with a complex etiology involving the failure of gallbladder formation during embryogenesis. Clinical manifestations are diverse; most patients are asymptomatic, while some present with symptoms such as biliary colic. The complexity of its clinical presentation and radiological features renders diagnosis challenging.

Case presentation

Fetal ultrasound at 22 weeks of gestation revealed an absent gallbladder. At 9 years and 11 months of age, the child exhibited significant weight gain and abnormalities. Abdominal ultrasound and magnetic resonance images demonstrated fatty liver and gallbladder agenesis. Liver function tests indicated mild abnormalities, with aspartate aminotransferase at 67 IU/L and alanine aminotransferase at 44 IU/L. Following 6 months of hepatoprotective and lipid-lowering therapy, a satisfactory treatment response was achieved, with normalization of liver function and improvement in fatty liver.

Conclusions

CAGB may be associated with other congenital abnormalities, although isolated cases are uncommon. Clinically, it may manifest as nonspecific biliary, gastrointestinal, or urinary symptoms, mimicking various digestive disorders and leading to misdiagnosis. Genetic sequencing and in-depth embryological research may elucidate the etiology and enhance diagnostic accuracy.
Colquhounia root tablet improves diabetic kidney disease by regulating epithelial-mesenchymal transition via the PTEN/PI3K/AKT pathway
Authors: Ma Donghong , Zhang Jiao , Du Lu , Shi Jingjing , Liu Zhaoyan , Qin Jilin , Chen Xiaoxiao , Guo Minghao
Published: July 2024

Background

Diabetic kidney disease (DKD) is a severe microvascular complication of diabetes mellitus that can lead to end-stage renal disease. Colquhounia root tablet (CRT) has shown therapeutic potential in treating DKD, but its efficacy and underlying mechanisms remain to be elucidated.

Methods

A randomized controlled clinical trial was conducted on 61 DKD patients. The treatment group received CRT in addition to standard therapy, while the control group received standard therapy alone. Treatment efficacy and adverse events were evaluated after 3 months. Additionally, in vitro experiments using human renal tubular epithelial cells (HK-2) were performed to investigate the effect of CRT on high glucose (HG)-induced epithelial-mesenchymal transition (EMT) and the involvement of the PTEN/PI3K/AKT signaling pathway.

Results

CRT treatment significantly improved proteinuria and increased the effective treatment rate in DKD patients compared to the control group, with no significant difference in adverse events. Moreover, CRT reversed HG-induced EMT in HK-2 cells, as evidenced by the downregulation of α-SMA and upregulation of E-cadherin at both mRNA and protein levels. Mechanistically, CRT increased PTEN expression and inhibited the PI3K/AKT pathway, similar to the effects of the PI3K inhibitor LY29400. The combination of CRT and LY29400 further enhanced PTEN mRNA expression under HG conditions.

Conclusion

CRT effectively improves proteinuria in DKD patients and ameliorates HG-induced EMT in HK-2 cells. The underlying mechanism may involve the upregulation of PTEN and subsequent inhibition of the PI3K/AKT signaling pathway. These findings provide new insights into the therapeutic potential of CRT for DKD treatment.
Anti-inflammatory properties of polysaccharides from edible fungi on health-promotion: a review
Authors: Yin Zhenhua , Zhang Juanjuan , Qin Jingjing , Guo Lin , Guo Qingfeng , Kang Wenyi , Ma Changyang , Chen Lin
Published: July 2024

Edible fungus polysaccharides have garnered significant attention from scholars due to their safety and potential anti-inflammatory activity. However, comprehensive summaries of their anti-inflammatory properties are still rare. This paper provides a detailed overview of the anti-inflammatory effects and mechanisms of these polysaccharides, as well as their impact on inflammation-related diseases. Additionally, the relationship between their structure and anti-inflammatory activity is discussed. It is believed that this review will greatly enhance the understanding of the application of edible fungus polysaccharides in anti-inflammatory treatments, thereby significantly promoting the development and utilization of edible fungi.
Abundant resources compensate for the uneven distribution of ungulates in desert grassland
Authors: Gou Xiaowei , Tsunekawa Atsushi , Tsubo Mitsuru , Peng Fei , Cheng Yunxiang
Published: July 2024

Introduction

Strategically managing livestock grazing in arid regions optimizes land use and reduces the damage caused by overgrazing. Controlled grazing preserves the grassland ecosystem and fosters sustainability despite resource limitations. However, uneven resource distribution can lead to diverse grazing patterns and land degradation, particularly in undulating terrains.

Methods

In this study, we developed a herbivore foraging algorithm based on a resource selection function model to analyze foraging distribution patterns, predict the probability of foraging, and identify the determinants of foraging probability in cattle. The study area was a complex desert landscape encompassing dunes and interdunes. Data on cattle movements and resource distribution were collected and analyzed to model and predict foraging behavior.

Results

Our findings revealed that cattle prefer areas with abundant vegetation in proximity to water sources and avoid higher elevations. However, abundant resource availability mitigated these impacts and enhanced the role of water points, particularly during late grazing periods. The analysis showed that available resources primarily determine foraging distribution patterns and lessen the effects of landforms and water distance on patch foraging.

Discussion

The results suggest that thoughtful water source placement and the subdivision of pastures into areas with varied terrain are crucial for sustainable grazing management. By strategically managing these factors, land degradation can be minimized, and the ecological balance of grassland ecosystems can be maintained. Further research is needed to refine the model and explore its applicability in other arid regions.
A review of Legionella transmission risk in built environments: sources, regulations, sampling, and detection
Authors: Yao Xiao Hui , Shen Fan , Hao Jing , Huang Lu , Keng Bin
Published: July 2024

The risk of Legionella transmission in built environments remains a significant concern. Legionella can spread within buildings through aerosol transmission, prompting the exploration of airborne transmission pathways and proposing corresponding prevention and control measures based on building characteristics. To this end, a comprehensive literature review on the transmission risk of Legionella in built environments was performed. Four electronic databases (PubMed, Web of Science, Google Scholar, and CNKI) were searched from inception to March 2024 for publications reporting the risk of Legionella transmission in built environments. Relevant articles and gray literature reports were hand-searched, and 96 studies were finally included. Legionella pollution comes from various sources, mainly originates in a variety of built environments in which human beings remain for extended periods. The sources, outbreaks, national standards, regulations, and monitoring techniques for Legionella in buildings are reviewed, in addition to increases in Legionella transmission risk due to poor maintenance of water systems and long-distance transmission events caused by aerosol characteristics. Air and water sampling using various analytical methods helps identify Legionella in the environment, recognize sources in the built environments, and control outbreaks. By comparing the standard regulations of national organizations globally, the authors further highlight gaps and deficiencies in Legionella surveillance in China. Such advancements offer essential insights and references for understanding and addressing Legionella transmission risk in the built environment, with the potential to contribute to safeguarding public health and building environment safety.
The impact of digital governance on the health of rural residents: the mediating role of governance efficiency and access to information
Authors: Chen Yongzhou , Ye Qiuzhi
Published: July 2024

Background

Digital transformation in rural areas has become a key policy priority worldwide. China is also implementing a digital village strategy and actively promoting the digital transformation of rural governance to improve the well-being of rural residents. The literature suggests that digital governance is linked to health, but the mechanisms behind this relationship remain unclear.

Methods

Using data from the 2021 China Land Economic Survey (CLES), this paper examines the impact of digital governance on the health, longevity, and mental health of rural residents. To enhance the robustness of the conclusions, this paper also introduces a dual machine learning model to solve the endogeneity problem of the model.

Conclusion and discussion

This study concludes that digital governance has a significant positive impact on the health of rural residents. This finding remains consistent even after addressing endogeneity issues and conducting numerous robustness tests. Mechanistic analyses indicate that digital governance can enhance rural residents’ health by improving village governance (environmental governance) and increasing the efficiency of access to personal information. Further analysis reveals that digital governance significantly increases the life expectancy of rural residents but that its effect on mental health is not significant. This study provides new insights into how digital governance affects health, with important implications for health policy development.
The association between weight-adjusted-waist index and muscle strength in adults: a population-based study
Authors: Xu Lihan , Zhou Haojing
Published: July 2024

Background

The relationship between the weight-adjusted-waist index (WWI) and grip strength, a crucial marker in assessing sarcopenia, lacks clarity. We aimed to explore the relationship between WWI and muscle strength across genders.

Methods

The cross-sectional study involved adults with complete data on WWI and grip strength from the 2011–2014 National Health and Nutrition Examination Survey. WWI was derived by dividing waist circumference by the square root of weight. Weighted multivariable logistic regression and smooth curve fitting techniques were used to examine the independent association and potential non-linear relationship between WWI and grip strength. A two-piecewise linear regression model was utilized to determine the threshold effect. Additionally, subgroup analyses and interaction tests were conducted.

Results

The study encompassed 9,365 participants, including 4,661 males and 4,704 females. Multivariate logistic regression analysis revealed a negative correlation between WWI and grip strength among males (β = −11.49, 95% CI: −12.38, −10.60, p < 0.001) as well as females (β = −2.53, 95% CI: −2.98, −2.08, p < 0.001). Subgroup analysis showed that the negative correlation of WWI with grip strength remained consistent across various age groups and levels of obesity for both males and females.

Conclusion

An increase in WWI correlates with reduced muscle strength in both males and females. WWI was negatively associated not only with muscle mass but also with muscle strength. WWI may serve as an assessment tool for sarcopenia, but further large-scale studies are needed to clarify causality.
Sustainable urban growth patterns based on environmental fitness
Authors: Ugalde-Monzalvo Marisol
Published: July 2024

This study examines the sustainability of urban growth, described by patterns of environmental fitness. The main assumption is that resource use—energy, materials, electricity, water, fossil fuels, soil, and humans—describes growth patterns whose sustainability can be categorized according to environmental fitness, which is assessed by the availability of environmental resources (characteristics of the environment) and the adaptation of the city to this availability (characteristics of the built environment). The article offers an innovative perspective by proposing a model for categorizing the sustainability of urban growth based on environmental fitness, but also by providing a mean to understand the city as a process and the city as a satisfier of needs. The methodology comprises two parts: (1) creating a matrix of indicators of urban environmental fitness and (2) constructing an urban growth sustainability index. From this methodology, six patterns emerged: (i) Economizing growth: available resources with maximum urban adaptation; (ii) Weak growth: availability of resources with minimal urban adaptation; (iii) Efficient growth: availability of resources with appropriate urban adaptation; (iv) Deficient growth: availability of resources without urban adaptation; (v) Efficient growth and of investment: lack of resources with urban investment; and (vi) Deficient growth and of degradation: lack of resources with urban deterioration and wear. The finding of these sustainable urban growth patterns demonstrates the concrete application of environmental adaptation theories and an understanding of the global behavior of cities. The empirical results support the assertion that urban growth presents challenges and potentials in terms of reduction, of reuse, and recycling; of urban sprawl, urban renewal, redevelopment and infill growth, and the efficiency and maintenance of urban infrastructure as guidelines for urban sustainability.
A near-real-time tropical deforestation monitoring algorithm based on the CuSum change detection method
Authors: Ygorra Bertrand , Frappart Frédéric , Wigneron Jean-Pierre , Catry Thibault , Pillot Benjamin , Pfefer Antoine , Courtalon Jonas , Riazanoff Serge
Published: July 2024

Tropical forests are currently under pressure from increasing threats. These threats are mostly related to human activities. Earth observations (EO) are increasingly used for monitoring forest cover, especially synthetic aperture radar (SAR), that is less affected than optical sensors by atmospheric conditions. Since the launch of the Sentinel-1 satellites, numerous methods for forest disturbance monitoring have been developed, including near real-time (NRT) operational algorithms as systems providing early warnings on deforestation. These systems include Radar for Detecting Deforestation (RADD), Global Land Analysis and Discovery (GLAD), Real Time Deforestation Detection System (DETER), and Jica-Jaxa Forest Early Warning System (JJ-FAST). These algorithms provide online disturbance maps and are applied at continental/global scales with a Minimum Mapping Unit (MMU) ranging from 0.1 ha to 6.25 ha. For local operators, these algorithms are hard to customize to meet users’ specific needs. Recently, the Cumulative sum change detection (CuSum) method has been developed for the monitoring of forest disturbances from long time series of Sentinel-1 images. Here, we present the development of a NRT version of CuSum with a MMU of 0.03 ha. The values of the different parameters of this NRT CuSum algorithm were determined to optimize the detection of changes using the F1-score. In the best configuration, 68% precision, 72% recall, 93% accuracy and 0.71 F1-score were obtained.
Fish kills and insecticides: historical water quality patterns in 10 agricultural watersheds in Prince Edward Island, Canada (2002–2022)
Authors: Crawford Miranda , Alexander Alexa C.
Published: July 2024

Global pesticide use has resulted in widespread environmental degradation, persistent contamination of surface and ground waters, bioaccumulation of these contaminants in food webs, and unintended impacts on non-target species (e.g., fish kills). Mixtures of multiple pesticides are commonly found in the environment, yet these chemicals are rarely studied in combination. Insecticides are of particular concern as these chemicals are designed to target terrestrial insect pests but also impact aquatic macroinvertebrates. In the following study, we explore the regional context of surface water concentrations of four insecticides in 10 study watersheds over a 20-year period (2002 to 2022) in Prince Edward Island, a region of Canada with continuous agricultural activity since the 1720s. These agroecosystems have been subject to generations of restoration, conservation, and more recently, implementation of various beneficial or best management practices (BMPs). The changing climate significantly adds to the complexity of monitoring these systems as the regional rate of change is exceptionally high (e.g., a 0.70°C to 1.14°C increase in air temperature and 5–8% decrease in precipitation in the last 3 decades). The results of this study highlight that efforts in this area would benefit from a more collaborative, transdisciplinary approach that integrates local, regional, national, and global perspectives while respecting the needs of growers, consumers, and the immense natural capital in the aquatic ecosystems draining these landscapes - such as Atlantic salmon, that can draw both anglers and ecotourists alike. A pivot toward a One-Health Framework is a logical next step for the province as, at present, efforts to integrate observational and monitoring efforts are already conducted by multiple federal, provincial, Indigenous rightsholders, as well as non-government stakeholders. Finally, in recognition that financial resources for these efforts are finite, we would recommend the implementation of a standardized, seasonal water quality sampling scheme that includes flow-weighted sampling and automated samplers to better capture and predict rapidly changing conditions in the region in response to climate change.
Case Report: Vertical muscle-sparing latissimus dorsi flap in the reconstruction of chronic radiation-induced chest wall ulcers after breast cancer surgery: a case series
Authors: Han Sung Joon , Kim Junghee , Kim Sunje , Ha Yooseok
Published: July 2024

Introduction

Radiation therapy, a standard postoperative treatment for breast cancer, can lead to chronic ulcers owing to compromised tissue healing. Accordingly, flap surgery using healthy tissues is essential for aesthetic and functional recovery. Although various flap techniques exist, each has its own drawbacks. This study introduces the vertical muscle-sparing latissimus dorsi flap as a superior alternative due to its comparative operative efficiency and tissue preservation.

Case report

Two female patients aged 60 and 59 years with histories of breast cancer in their left breast treated with radiotherapy presented with chronic ulcers. The first patient had a 4 × 5 cm defect infiltrating the pleural space, while the second had a 15 × 9 cm defect after thoracic surgery for a bronchopleural fistula. In both cases, debridement was followed by reconstruction using a vertical muscle-sparing latissimus dorsi flap, thereby avoiding the need to change the patient's position and repeated draping during surgery. Both patients showed good postoperative recovery without significant complications.

Results

The vertical muscle-sparing latissimus dorsi flap resulted in better adhesions and functional outcomes due to shorter surgical duration.

Conclusion

The vertical muscle-sparing latissimus dorsi flap is an effective and efficient method for reconstructing radiation-induced chest wall ulcers in patients with breast cancer. Its application in the presented cases highlights its potential as a preferred option in similar clinical scenarios.
A decade of tuberculosis eradication programs in the Mediterranean water buffalo (Bubalus bubalis) in South Italy: Are we heading toward eradication?
Authors: Martucciello Alessandra , Ottaiano Maria , Mazzone Piera , Vitale Nicoletta , Donniacuo Anna , Brunetti Roberta , Di Franco Marcellino , Cerrone Pasquale , Spoleto Claudia , Galiero Giorgio , De Carlo Esterina
Published: July 2024

The water buffalo (Bubalus bubalis) is susceptible to bovine tuberculosis (TB), which receives increased attention in areas where buffalo breeding is prevalent, such as in Southern Italy, especially in the Campania region, where 70% of the buffalo stock is bred. Since 2012, TB testing in buffalo herds has been conducted using the Single Intradermal Test (SIT), with the Comparative Intradermal test (CIT) used in cases of inconclusive results. From 2012 to 2016, the interferon-gamma (IFN-γ) test was occasionally employed experimentally in herds with TB outbreaks to expedite eradication efforts. A local TB eradication program was implemented in officially TB-free buffalo herds between 2017 and 2019. This program involves initial screening with SIT, followed by confirmatory tests, including CIT and IFN-γ, for positive reactions. Since June 2019, the IFN-γ test has replaced the CIT in officially TB-free herds upon positive SIT reactions. Additionally, in suspected and confirmed TB-outbreak herds, the IFN-γ test was used at the discretion of the competent authority. Between 2017 and 2019, approximately 295,000 buffaloes in Campania were screened annually with in vivo tests provided by TB eradication programs. During this period, 32,040 animals from 855 herds were tested using the IFN-γ test and 4,895 tested positive. Since 2020, the use of IFN-γ testing has increased, and has become a prerequisite for the acquisition of TB-free status and is being systematically applied for TB outbreak-extinction procedures. The test was performed in all breeding buffaloes in cases of doubtful SIT results in TB-free herds and when TB lesions are detected at slaughter in animals from TB-free herds. This combined approach helped detect more TB outbreaks, and thereby led to a reduction in the TB prevalence and incidence rates. By 2022, the prevalence had decreased to 1.56%, and the incidence had decreased to 0.73%, after the increased use of the IFN-γ test. This study highlights the effectiveness of implemented strategies in reducing TB in this region. Overall, the data demonstrate the successful impact of TB eradication measures and surveillance activities in reducing bubaline TB prevalence and incidence in the Campania region.
Transient efficacy of buparvaquone against the US isolate of Theileria orientalis Ikeda genotype in sub-clinically infected cattle
Authors: Bastos Reginaldo G. , Hassan Amany , Onzere Cynthia K. , Herndon David R. , Villarino Nicolas F. , Laughery Jacob M. , Fry Lindsay M.
Published: July 2024

Introduction

Theileria orientalis, an economically significant tick-borne hemoparasite, infects cattle globally. The T. orientalis Ikeda genotype, transmitted by Haemaphysalis longicornis ticks, is associated with clinical manifestations characterized by anemia, abortions, and mortality, although subclinical infections prevail. Despite the common occurrence of subclinical infections, therapeutic interventions targeting T. orientalis Ikeda in such cases are currently lacking, impeding effective parasite control measures. To address this critical knowledge gap, we assessed the efficacy of buparvaquone (BPQ) in eliminating the T. orientalis Ikeda, US isolate, in sub-clinically infected cattle.

Methods

Twelve sub-clinically infected calves, identified by the presence of T. orientalis in peripheral blood alongside the absence of fever and anemia, were enrolled in the study. Six calves received two treatments of the BPQ label dose (2.5 mg/kg) at a 48-h interval, while additional three calves received the drug at a dosage of 6 mg/kg following the same regimen. Three untreated calves served as controls.

Results and discussion

Endpoint and quantitative PCR analyses revealed that BPQ exerted a transient effect on T. orientalis parasitemia. Parasites remained undetectable in peripheral blood until weeks 4 and 11 post-treatment in animals administered 2.5 mg/kg and 6 mg/kg of BPQ, respectively. Intriguingly, following recrudescence, administering 6 mg/kg to animals previously treated with 2.5 mg/kg did not result in a reduction in parasite load. Pharmacokinetic analysis data suggested that escalating the dosage led to a less than proportional increase in serum concentrations of BPQ. Moreover, a significant yet reversible decrease (p < 0.05) in blood urea nitrogen was observed in animals treated with the drug, irrespective of the dosage. Despite parasitemia relapse, animals treated with 6 mg/kg BPQ exhibited a noteworthy decrease (p < 0.05) in IgG levels specific to the T. orientalis major piroplasm surface protein compared to controls and animals treated with 2.5 mg/kg of the drug.

Conclusion

BPQ did not demonstrate efficacy in clearing subclinical T. orientalis Ikeda infection. Future investigations are warranted to explore innovative therapeutic modalities that, in synergy with vaccines and diagnostic assays, can facilitate the development of comprehensive programs aimed at controlling and eradicating this parasite.
Advancing the Diagnosis of Aero-Engine Bearing Faults with Rotational Spectrum and Scale-Aware Robust Network
Authors: Li Jin , Yang Zhengbing , Zhou Xiang , Song Chenchen , Wu Yafeng
Published: July 2024

The precise monitoring of bearings is crucial for the timely detection of issues in rotating mechanical systems. However, the high complexity of the structures makes the paths of vibration signal transmission exceedingly intricate, posing significant challenges in diagnosing aero-engine bearing faults. Therefore, a Rotational-Spectrum-informed Scale-aware Robustness (RSSR) neural network is proposed in this study to address intricate fault characteristics and significant noise interference. The RSSR algorithm amalgamates a scale-aware feature extraction block, a non-activation convolutional network, and an innovative channel attention block, striking a balance between simplicity and efficacy. We provide a comprehensive analysis by comparing traditional CNNs, transformers, and their respective variants. Our strategy not only elevates diagnostic precision but also judiciously moderates the network’s parameter count and computational intensity, mitigating the propensity for overfitting. To assess the efficacy of our proposed network, we performed rigorous testing using two complex, publicly available datasets, with additional artificial noise introductions to simulate challenging operational environments. On the noise-free dataset, our technique increased the accuracy by 5.11% on the aero-engine dataset compared with the current mainstream methods. Even under maximal noise conditions, it enhances the average accuracy by 4.49% compared with other contemporary approaches. The results demonstrate that our approach outperforms other techniques in terms of diagnostic performance and generalization ability.
Effects of Abscisic Acid on Rice Seed Dormancy: Antioxidant Response and Accumulations of Melatonin, Phenolics and Momilactones
Authors: Rayee Ramin , Anh La Hoang , Xuan Tran Dang
Published: July 2024

Abscisic acid (ABA) is a well-known phytohormone that initiates seed dormancy. This is the first study elucidating the variability and relationship in the accumulation of phenolics, melatonin, and momilactones A (MA) and B (MB) during a rice (Oryza sativa L. var. Koshihikari, Akisakari, and Akiroman) seed dormancy process treated by ABA with concentrations of 0 (control), 10, 50, and 100 µM over 8 days. Accordingly, increased concentrations of ABA resulted in an extended dormancy period of rice seeds, along with reducing fresh weight while maintaining stable dry weight in all varieties. ABA treatment elevated total phenolic (TPC) and flavonoid (TFC) contents. Particularly, quantities of ferulic and cinnamic acids were enhanced, in line with the promoted antioxidant capacities of ABA-treated rice seeds. Momilactone contents were increased (3.8% to 32.6% for MA and 16.3% to 31.3% for MB) during the extension of rice seed dormancy regulated by ABA. Notably, the accumulation tendency of MA and MB under ABA effects was consistent with that of melatonin, a phytohormone associated with seed dormancy prolongation. The finding implies that MA and MB may play a role alongside melatonin in signaling the extended dormancy of rice seeds through the ABA pathway. Future research should delve into the molecular mechanisms underlying these observations.
Deep Learning with a Multi-Task Convolutional Neural Network to Generate a National-Scale 3D Soil Data Product: The Particle Size Distribution of the German Agricultural Soil Landscape
Authors: Ließ Mareike , Sakhaee Ali
Published: July 2024

Many soil functions and processes are controlled by the soil particle size distribution. Accordingly, nationwide geoinformation on this soil property is required to enable climate-smart and resilient land management. This study presents a new deep learning approach to simultaneously model the contents of the three particle sizes of sand, silt, and clay and their variations with depth throughout the landscape. The approach allows for the consideration of the natural soil horizon boundaries and the inclusion of the surrounding landscape context of each soil profile to investigate the soil–landscape relation. Applied to the agricultural soil landscape of Germany, the approach generated a three-dimensional continuous data product with a resolution of 100 m in geographic space and a depth resolution of 1 cm. The approach relies on a patch-wise multi-target convolutional neural network (CNN) model. Genetic algorithm optimization was applied for CNN parameter tuning. Overall, the effectiveness of the CNN algorithm in generating multidimensional, multivariate, national-scale soil data products was demonstrated. The predictive performance resulted in a median root mean square error of 17.8 mass-% for the sand, 14.4 mass-% for the silt, and 9.3 mass-% for the clay content in the top ten centimeters. This increased to 20.9, 16.5, and 11.8 mass-% at a 40 cm depth. The generated data product is the first of its kind. However, even though the potential of this deep learning approach to understand and model the complex soil–landscape relation is virtually limitless, its limitations are data driven concerning the approximation of the soil-forming factors and the available soil profile data.
Response of Yam Yield and Soil Microbial Communities to Soil Fumigation and Substrate Cultivation
Authors: Jin Xi , Hao Zheng , Song Yelong , Gao Lan , He Fuqiang , Li Qingjie , Cao Aocheng
Published: July 2024

Soil fumigation is considered a method to control soil-borne diseases and solve crop continuous cropping obstacles. However, fumigant residues in the soil are detrimental to soil health. Though substrate cultivation is a cultivation mode that can promote plant growth, studies to date on whether substrate cultivation can replace soil fumigation for the control of soil pathogens are limited. In this study, the effects of chloropicrin fumigation (Pic) and substrate cultivation (SC) on yam growth, soil pathogens, soil nutrients, and microbial communities were demonstrated using a 2-year field experiment. The results showed that SC significantly increased the content of soil organic matter (SOM), available phosphorus, and available potassium compared with Pic. In addition, SC could effectively reduce the number of Fusarium spp. and Phytophthora spp., decrease the rate of diseased yam plants, and significantly increase the yam yield. Moreover, SC significantly increased the abundance of beneficial microorganisms such as Actinobacteriota, Acidobacteriota, and Bacillus in soil. Correlation analysis showed that yam yield exhibited a negative relation with the number of soil pathogens and a positive correlation with SOM. Our study suggests that substrate cultivation can be an alternative to soil fumigation to control soil pathogens and protect soil health.
Improving Sowing Uniformity of a Maize High-Speed Precision Seeder by Incorporating Energy Dissipator
Authors: Liu Rui , Wu Guangwei , Dong Jianjun , Yan Bingxin , Meng Zhijun
Published: July 2024

To enhance the sowing uniformity of the vacuum seeder in the high-speed working state, a flexible energy-dissipation receiving device was designed. We analyzed the angle and velocity of seed ejection from the seed-metering device. Additionally, we explored the rheological properties of four different sodium alginate (SA) solutions. Combined with high-speed camera technology, the movement characteristics of four kinds of energy dissipators were revealed, and it was determined that the fabrication material of the energy dissipator is colloid with an SA percentage of 10%. The influence of the thickness of the energy dissipator body, impact velocity, and impact angle on the pre- and post-impact velocity difference and end-of-motion transverse displacement value was investigated. The quadratic regression equation between experimental factors and experimental indexes was established, and it was determined that the thickness of the energy dissipator was 7 mm. Field experiment results showed that the working speed was 12~16 km·h−1, the leakage rate was less than 6.83%, the multiple rates were less than 0.97%, the qualified rate was stable at more than 92.4%, and the qualified grain distance variation rate was stable at less than 16.57%. The designed energy-dissipation device is beneficial to improve the overall working performance of high-speed precision seeders. In the future, if the reliability and long-term performance of the energy-dissipation device are further improved, it will be able to meet the requirements for precision seeding under high-speed conditions.
Relationship between Chilling Accumulation and Heat Requirement for Flowering in Peach Varieties of Different Chilling Requirements
Authors: Yan Juan , Cai Zhixiang , Chen Zheng , Zhang Binbin , Li Jiyao , Xu Jianlan , Ma Ruijuan , Yu Mingliang , Shen Zhijun
Published: July 2024

Previous studies have shown a negative correlation between chilling accumulation (CA) and heat requirements (HRs) in peaches (Prunus persica (L.) Batsch), consistent with findings in other plants in spring events. However, there is a lack of comparative research on the CA–HR relationship in peach varieties with different chilling requirements (CRs), and the specific impact of CA on HR reduction remains poorly described. To address this, we investigated the effects of CA on the days and HR for flowering in 54 peach varieties of differing CRs. Scanning electron microscopy was used to observe the effects of CA on the phenology of floral organ development in a selected peach variety. Our results indicate that, in general, peaches exhibit a reduced HR and accelerated flowering as the CA increases, but that the strength and trend of the CA–HR relationship is influenced by the CR and the variety. Low-CR varieties showed less sensitivity to CA increments, requiring higher relative increases in CA to significantly lower the HR, whereas high-CR varieties appeared to be more sensitive, with even modest changes leading to substantial reductions in HR. However, variations from this generality exist, even within varieties displaying the same rCA (the ratio of CA to CR). Additionally, we provide a summary of the relationship between the rCA and drHR in peaches of differing CRs, and identify several varieties exhibiting a strong response in the CA–HR relationship. This study also highlights the impact of CA on flower bud development, revealing slower progression under lower CA levels and accelerated growth with an increased CA. In particular, we identified the critical period of the enlargement and initiation of green scales as indicative of successful pollen grain formation. Finally, we present a schematic of the CA–HR relationship for flowering in peaches.
Borrow Pit Disposal of Coal Mining Byproducts Improves Soil Physicochemical Properties and Vegetation Succession
Authors: Bakr Jawdat , Kompała-Bąba Agnieszka , Bierza Wojciech , Chmura Damian , Hutniczak Agnieszka , Kasztowski Jacek , Jendrzejek Bartosz , Zarychta Adrian , Woźniak Gabriela
Published: July 2024

The way of disposing of rock mineral material has a significant impact on subsequent spontaneous vegetation succession, soil properties, and respiration. We compared seven spontaneously vegetated samples from a large (2 km2) borrow pit used to dispose of the byproducts of a hard coal mine with seven plots from four coal mine spoil heap piles. We used BIOLOG EcoPlates to assess the microbial catabolic activity of the substrate. The substrate in the borrow pit was characterized by higher water content and lower temperature compared to the heap pile substrate. The borrow pit had a more diverse plant community structure. Higher Rao’s quadratic entropy, functional richness, and functional divergence were also calculated from plant functional traits in borrow pit samples. Although borrow pit samples showed higher total microbial biomass, bacteria/fungi ratio, and gram+/gram− ratio, and heap pile samples showed higher soil enzymatic activity, microbial functional diversity, and catabolic activity, these differences were not significant. Soil respiration from the borrow pit substrate was two folds higher. The borrow pit method of disposing of rock mineral material can be suggested to speed up spontaneous vegetation succession. This research provides new insights into the effects of burying hard coal byproducts in borrow pits and offers guidance for the management of hard coal mining.
Establishment of a Steatosis Model in LMH Cells, Chicken Embryo Hepatocytes, and Liver Tissues Based on a Mixture of Sodium Oleate and Palmitic Acid
Authors: Zhuang Wuchao , Chen Ziwei , Shu Xin , Zhang Jilong , Zhu Runbang , Shen Manman , Chen Jianfei , Zheng Xiaotong
Published: July 2024

Research on hepatic steatosis in animal husbandry has been a prominent area of study. Developing an appropriate in vitro cellular steatosis model is crucial for comprehensively investigating the mechanisms involved in liver lipid deposition in poultry and for identifying potential interventions to address abnormalities in lipid metabolism. The research on the methods of in vitro liver steatosis in chickens, particularly the effects of different fat mixtures, is still lacking. In this study, LMH cells were utilized to investigate the effects of OA, SO, PA, SP, and their pairwise combinations on steatosis development, with the aim of identifying the optimal conditions for inducing steatosis. Analysis of triglyceride (TG) content in LMH cells revealed that OA and SP had limited efficacy in increasing TG content, while a combination of SO and PA in a 1:2 ratio exhibited the highest TG content. Moreover, Oil Red O staining results in LMH cells demonstrated that the combination treatment had a more pronounced induction effect compared to 0.375 mM SO. Additionally, RNA-seq analysis showed that 0.375 mM SO significantly influenced the expression of genes associated with fatty acid metabolism compared to the control group, whereas the combination of SO and PA led to an enrichment of key GO terms associated with programmed cell death. These findings suggest that varying conditions of cellular steatosis could lead to distinct disruptions in gene expression. The optimal conditions for inducing steatosis in LMH cells were also tested on chicken embryonic liver cells and embryos. TG detection and Oil Red O staining assays showed that the combination of SO and PA successfully induced steatosis. However, the gene expression pattern differed from that of LMH cells. This study lays the foundations for further investigations into avian hepatic steatosis.
Genome-Wide Association Study as an Efficacious Approach to Discover Candidate Genes Associated with Body Linear Type Traits in Dairy Cattle
Authors: Long Mingxue , Wang Bo , Yang Zhangping , Lu Xubin
Published: July 2024

Body shape traits are very important and play a crucial role in the economic development of dairy farming. By improving the accuracy of selection for body size traits, we can enhance economic returns across the dairy industry and on farms, contributing to the future profitability of the dairy sector. Registered body conformation traits are reliable and cost-effective tools for use in national cattle breeding selection programs. These traits are significantly related to the production, longevity, mobility, health, fertility, and environmental adaptation of dairy cows. Therefore, they can be considered indirect indicators of economically important traits in dairy cows. Utilizing efficacious genetic methods, such as genome-wide association studies (GWASs), allows for a deeper understanding of the genetic architecture of complex traits through the identification and application of genetic markers. In the current review, we summarize information on candidate genes and genomic regions associated with body conformation traits in dairy cattle worldwide. The manuscript also reviews the importance of body conformation, the relationship between body conformation traits and other traits, heritability, influencing factors, and the genetics of body conformation traits. The information on candidate genes related to body conformation traits provided in this review may be helpful in selecting potential genetic markers for the genetic improvement of body conformation traits in dairy cattle.