bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Analyses of Chloroplast Genome Provide Effective Molecular Markers for Species and Cultivar Identification in Bougainvillea
Authors: Lin Xinggu , Lee Shiou Yih , Ni Jianzhong , Zhang Xiaomin , Hu Xing , Zou Peishan , Wang Wei , Liu Guofeng
Published: October 2023

Bougainvillea is popular in ornamental horticulture for its colorful bracts and excellent adaptability, but the complex genetic relationship among this genus is fuzzy due to limited genomic data. To reveal more genomic resources of Bougainvillea, we sequenced and assembled the complete chloroplast (cp) genome sequences of Bougainvillea spectabilis ‘Splendens’. The cp genome size was 154,869 bp in length, containing 86 protein-coding genes, 38 tRNAs, and eight rRNAs. Cp genome comparison across 12 Bougainvillea species (B. spectabilis, B. glabra, B. peruviana, B. arborea, B. praecox, B. stipitata, B. campanulata, B. berberidifolia, B. infesta, B. modesta, B. spinosa, and B. pachyphylla) revealed five mutational hotspots. Phylogenetic analysis suggested that B. spectabilis published previously and B. glabra clustered into one subclade as two distinct groups, sister to the subclade of B. spectabilis ‘Splendens’. We considered the phylogeny relationships between B. spectabilis and B. glabra to be controversial. Based on two hypervariable regions and three common plastid regions, we developed five molecular markers for species identification in Bougainvillea and applied them to classify 53 ornamental Bougainvillea cultivars. This study provides a valuable genetic resource for Bougainvillea breeding and offers effective molecular markers to distinguish the representative ornamental species of Bougainvillea.
Erythroid Cells as Full Participants in the Tumor Microenvironment
Authors: Shevchenko Julia A. , Nazarov Kirill V. , Alshevskaya Alina A. , Sennikov Sergey V.
Published: October 2023

The tumor microenvironment is an important factor that can determine the success or failure of antitumor therapy. Cells of hematopoietic origin are one of the most important mediators of the tumor–host interaction and, depending on the cell type and functional state, exert pro- or antitumor effects in the tumor microenvironment or in adjacent tissues. Erythroid cells can be full members of the tumor microenvironment and exhibit immunoregulatory properties. Tumor growth is accompanied by the need to obtain growth factors and oxygen, which stimulates the appearance of the foci of extramedullary erythropoiesis. Tumor cells create conditions to maintain the long-term proliferation and viability of erythroid cells. In turn, tumor erythroid cells have a number of mechanisms to suppress the antitumor immune response. This review considers current data on the existence of erythroid cells in the tumor microenvironment, formation of angiogenic clusters, and creation of optimal conditions for tumor growth. Despite being the most important life-support function of the body, erythroid cells support tumor growth and do not work against it. The study of various signaling mechanisms linking tumor growth with the mobilization of erythroid cells and the phenotypic and functional differences between erythroid cells of different origin allows us to identify potential targets for immunotherapy.
Immunolocalization of Jasmonates and Auxins in Pea Roots in Connection with Inhibition of Root Growth under Salinity Conditions
Authors: Akhiyarova Guzel , Vafina Gyulnar , Veselov Dmitriy , Kudoyarova Guzel
Published: October 2023

Inhibition of root elongation is an important growth response to salinity, which is thought to be regulated by the accumulation of jasmonates and auxins in roots. Nevertheless, the mechanisms of the interaction of these hormones in the regulation of the growth response to salinity are still not clear enough. Their better understanding depends on the study of the distribution of jasmonates and auxins between root cells. This was achieved with the help of immunolocalization of auxin (indoleacetic acid) and jasmonates on the root sections of pea plants. Salinity inhibited root elongation and decreased the size of the meristem zone and the length of cells in the elongation zone. Immunofluorescence based on the use of appropriate, specific antibodies that recognize auxins and jasmonates revealed an increased abundance of both hormones in the meristem zone. The obtained data suggests the participation of either auxins or jasmonates in the inhibition of cell division, which leads to a decrease in the size of the meristem zone. The level of only auxin and not jasmonate increased in the elongation zone. However, since some literature evidence argues against inhibition of root cell division by auxins, while jasmonates have been shown to inhibit this process, we came to the conclusion that elevated jasmonate is a more likely candidate for inhibiting root meristem activity under salinity conditions. Data suggests that auxins, not jasmonates, reduce cell size in the elongation zone of salt-stressed plants, a suggestion supported by the known ability of auxins to inhibit root cell elongation.
Improved piggyBac Transformation with Capped Transposase mRNA in Pest Insects
Authors: Häcker Irina , Rehling Tanja , Schlosser Henrik , Mayorga-Ch Daniela , Heilig Mara , Yan Ying , Armbruster Peter A. , Schetelig Marc F.
Published: October 2023

Creating transgenic insects is a key technology in insect genetics and molecular biology. A widely used instrument in insect transgenesis is the piggyBac transposase, resulting in essentially random genomic integrations. In contrast, site-specific recombinases allow the targeted integration of the transgene construct into a specific genomic target site. Both strategies, however, often face limitations due to low transgenesis efficiencies. We aimed to enhance transgenesis efficiencies by utilizing capped mRNA as a source of transposase or recombinase instead of a helper plasmid. A systematic comparison of transgenesis efficiencies in Aedes mosquitoes, as models for hard-to-transform insects, showed that suppling piggyBac transposase as mRNA increased the average transformation efficiency in Aedes aegypti from less than 5% with the plasmid source to about 50% with mRNA. Similar high activity was observed in Ae. albopictus with pBac mRNA. No efficiency differences between plasmid and mRNA were observed in recombination experiments. Furthermore, a hyperactive version of piggyBac transposase delivered as a plasmid did not improve the transformation efficiency in Ae. aegypti or the agricultural pest Drosophila suzukii. We believe that the use of mRNA has strong potential for enhancing piggyBac transformation efficiencies in other mosquitoes and important agricultural pests, such as tephritids.
Insensitive Effects of Inflammatory Cytokines on the Reference Genes of Synovial Fluid Resident-Mesenchymal Stem Cells Derived from Rheumatoid Arthritis Patients
Authors: Bok Eun-Yeong , Kim Saet-Byul , Thakur Gitika , Choe Yong-Ho , Oh Seong-Ju , Hwang Sun-Chul , Ock Sun-A. , Rho Gyu-Jin , Lee Sang-Il , Lee Won-Jae , Lee Sung-Lim
Published: October 2023

Mesenchymal stem cells derived from rheumatoid arthritis patients (RA-MSCs) provide an understanding of a variety of cellular and immunological responses within the inflammatory milieu. Sustained exposure of MSCs to inflammatory cytokines is likely to exert an influence on genetic variations, including reference genes (RGs). The sensitive effect of cytokines on the reference genes of RA-SF-MSCs may be a variation factor affecting patient-derived MSCs as well as the accuracy and reliability of data. Here, we comparatively evaluated the stability levels of nine RG candidates, namely GAPDH, ACTB, B2M, EEF1A1, TBP, RPLP0, PPIA, YWHAZ, and HPRT1, to find the most stable ones. Alteration of the RG expression was evaluated in MSCs derived from the SF of healthy donors (H-SF-MSCs) and in RA-SF-MSCs using the geNorm and NormFinder software programs. The results showed that TBP, PPIA, and YWHAZ were the most stable RGs for the normalization of H-SF-MSCs and RA-SF-MSCs using RT-qPCR, whereas ACTB, the most commonly used RG, was less stable and performed poorly. Additionally, the sensitivity of RG expression upon exposure to proinflammatory cytokines (TNF-α and IL-1β) was evaluated. RG stability was sensitive in the H-SF-MSCs exposed to TNF-α and IL-1β but insensitive in the RA-SF-MSCs. Furthermore, the normalization of IDO expression using ACTB falsely diminished the magnitude of biological significance, which was further confirmed with a functional analysis and an IDO activity assay. In conclusion, the results suggest that TBP, PPIA, and YWHAZ can be used in SF-MSCs, regardless of their exposure to inflammatory cytokines.
Synthesis and Characterization of Antibacterial Chitosan Films with Ciprofloxacin in Acidic Conditions
Authors: Sikorski Dominik , Rosiak Piotr , Janczewski Łukasz , Potrzebowski Marek J. , Kregiel Dorota , Kaźmierski Sławomir , Neubauer Damian , Kolesińska Beata , Frączyk Justyna , Adamczyk Anna , Draczyński Zbigniew
Published: October 2023

This work presents the results of research on obtaining chitosan (CS) films containing on their surface ciprofloxacin (CIP). A unique structure was obtained that not only gives new properties to the films, but also changes the way of coverage and structure of the surface. The spectroscopic test showed that in the process of application of CIP on the surface of CS film, CIP was converted from its crystalline form to an amorphic one, hence improving its bioavailability. This improved its scope of microbiological effect. The research was carried out on the reduction of CIP concentration during the process of CIP adhesion to the surface of chitosan films. The antibacterial activity of the CS films with and without the drug was evaluated in relation to Escherichia coli and Staphylococcus aureus, as well as Candida albicans and Penicillium expansum. Changes in the morphology and roughness of membrane surfaces after the antibacterial molecule adhesion process were tested with atomic force microscopy (AFM) and scanning electron microscopy (SEM). Structural analysis of CS and its modifications were confirmed with Fourier-transform spectroscopy in the infrared by an attenuated total reflectance of IR radiation (FTIR-ATR) and solid-state nuclear magnetic resonance (NMR).
GaAs Quantum Dot Confined with a Woods–Saxon Potential: Role of Structural Parameters on Binding Energy and Optical Absorption
Authors: Dakhlaoui Hassen , Belhadj Walid , Elabidi Haykel , Ungan Fatih , Wong Bryan M.
Published: October 2023

We present the first detailed study of optical absorption coefficients (OACs) in a GaAs quantum dot confined with a Woods–Saxon potential containing a hydrogenic impurity at its center. We use a finite difference method to solve the Schrödinger equation within the framework of the effective mass approximation. First, we compute energy levels and probability densities for different parameters governing the confining potential. We then calculate dipole matrix elements and energy differences, E1p−E1s, and discuss their role with respect to the OACs. Our findings demonstrate the important role of these parameters in tuning the OAC to enable blue or red shifts and alter its amplitude. Our simulations provide a guided path to fabricating new optoelectronic devices by adjusting the confining potential shape.
Niche and Range Shifts of Aedes aegypti and Ae. albopictus Suggest That the Latecomer Shows a Greater Invasiveness
Authors: Nie Peixiao , Feng Jianmeng
Published: October 2023

The yellow fever (Aedes aegypti) and Asian tiger (Ae. albopictus) mosquitos are major vectors of global mosquito-borne pathogens. However, their niche and range shifts, the underlying mechanisms, and related relative invasion rates remain scarcely known. We examined the niche and range shifts between the native and invasive Ae. aegypti and Ae. albopictus populations through dynamic niche and range models and the largest occurrence record datasets to date. We detected substantial niche and range expansions in both species, probably because the introduced populations have more opportunities to acclimate to diverse environmental conditions than their native counterparts. Mitigating climate change could effectively control their future invasions, given that future climate changes could promote their invasiveness. Additionally, compared to the introduced Ae. aegypti, the more recent invader Ae. albopictus had greater niche and range expansion over its shorter invasion history. In terms of the range shifts, Ae. albopictus had an invasion rate approximately 13.3 times faster than that of Ae. aegypti, making it a more invasive vector of global mosquito-borne pathogens. Therefore, considering its higher invasion rate, much more attention should be paid to Ae. albopictus in devising our strategies against prevailing global mosquito-borne pathogens than Ae. aegypti. Since small niche shifts could result in their large range shifts, niche shifts might be a more important indicator for biological invasion assessments.
Dactylopius opuntiae [Cockerell] Could Be a Source of Antioxidants for the Preservation of Beef Patties
Authors: Aragon-Martinez Othoniel H. , Martinez-Morales Flavio , González-Chávez Marco M. , Méndez-Gallegos Santiago de J. , González-Chávez Rodolfo , Posadas-Hurtado Juan C. , Isiordia-Espinoza Mario A.
Published: October 2023

Dactylopius opuntiae is an insect pest that contains at least carminic acid, which has antioxidant properties. Since there is a relationship between the antioxidant ability and preservative action of compounds applied to meat products, the purpose of this study was to evaluate the antioxidant activity and usefulness of a D. opuntiae extract for beef patty preservation. The insects were bred and processed to obtain a liquid extract. For the extract, its carminic acid content, antioxidant activity against two free radicals, and actions on food quality parameters were determined. The D. opuntiae dry powder contained 2.91% w/w carminic acid, while the liquid extract exhibited an IC50 value of 3437.8 ± 67.8 and 19633.0 ± 674.5 µg/mL against the DPPH and ABTS radicals. Nevertheless, these antioxidant actions were lower than those found in a D. coccus extract. The D. opuntiae extract improved in a short time the redness and yellowness, eliminated the unfavorable effect of their vehicle on the MetMb level, and greatly reduced the TBARS formation. For the first time, an extract of D. opuntiae was applied to beef patties, and its beneficial antioxidant action on meat acceptance parameters was confirmed, which has potential commercial applications.
Reproductive Outcomes of Women with Turner Syndrome Undergoing Oocyte Vitrification: A Retrospective Multicenter Cohort Study
Authors: Nadesapillai Sapthami , Mol Femke , Broer Simone L. , Stevens Brentjens Linda B. P. M. , Verhoeven Marieke O. , Heida Karst Y. , Goddijn Mariëtte , van Golde Ron J. T. , Bos Annelies M. E. , van der Coelen Sanne , Peek Ronald , Braat Didi D. M. , van der Velden Janielle A. E. M. , Fleischer Kathrin
Published: October 2023

Background: Turner syndrome (TS) is accompanied with premature ovarian insufficiency. Oocyte vitrification is an established method to preserve fertility. However, data on the oocyte yield in women with TS who vitrify their oocytes and the return rate to utilize the oocytes are scarce. Methods: Retrospective multicenter cohort study. Data was collected from medical records of women with TS who started oocyte vitrification between 2010 and 2021. Results: Thirty-three women were included. The median cumulative number of vitrified oocytes was 20 per woman. Complications occurred in 4% of the cycles. Significant correlations were found between the cumulative number of vitrified oocytes and AMH (r = 0.54 and p < 0.01), AFC (r = 0.49 and p < 0.01), percentage of 46,XX cells (r = 0.49 and p < 0.01), and FSH (r = −0.65 and p < 0.01). Spontaneous (n = 8) and IVF (n = 2) pregnancies occurred in 10 women ± three years after vitrification. So far, none of the women have returned to utilize their vitrified oocytes. Conclusions: Oocyte vitrification is a feasible fertility preservation option for women with TS, particularly in those with 46,XX cell lines or sufficient ovarian reserve. Multiple stimulation cycles are recommended to reach an adequate number of vitrified oocytes for pregnancy. It is too early to draw conclusions about the utilization of vitrified oocytes in women with TS.
Long-Term Clinical Implications of Atrial Fibrillation on Mortality in Patients Hospitalized with COVID-19: A Nationwide Cohort Study
Authors: Lim Kyoung Ree , Lee Seunghwa , Kim Bum Sung , Chun Kwang Jin
Published: October 2023

Background: Atrial fibrillation (AF) increases the risk of long-term mortality in patients hospitalized with Coronavirus Disease 2019 (COVID-19), but the evidence is limited. Methods: This study used data from the Common Data Model of the Health Insurance Review and Assessment Service of Korea collected between 1 January 2020 and 30 April 2022. A total of 107,247 patients hospitalized with COVID-19 were included in this study. They were divided into two groups according to a history of AF. The primary outcome was all-cause mortality. Results: After propensity score stratification, 1919 patients with a history of AF and 105,328 patients without a history of AF who were hospitalized with COVID-19 were analyzed to determine long-term mortality. The primary outcome occurred in 99 of 1919 patients (5.2%) with a history of AF and in 1397 of 105,328 patients (1.3%) without a history of AF (hazard ratio, 1.49; 95% confidence interval 1.20–1.82; p < 0.01). A history of AF was also associated with an increased risk of within 30-day mortality. Conclusion: A history of AF was associated with an increased risk of long-term mortality in patients hospitalized with COVID-19. Our findings indicate the necessity for physicians to reevaluate the optimal management of patients with AF following discharge.
Mapping the Most Common Founder Variant in RSPH9 That Causes Primary Ciliary Dyskinesia in Multiple Consanguineous Families of Bedouin Arabs
Authors: Al-Mutairi Dalal A. , Alsabah Basel H. , Pennekamp Petra , Omran Heymut
Published: October 2023

Introduction: Primary ciliary dyskinesia (PCD) is a congenital thoracic disorder caused by dysfunction of motile cilia, resulting in insufficient mucociliary clearance of the lungs. The overall aim of this study is to identify causative defective genes in PCD-affected individuals in the Kuwaiti population. Methods: A cohort of multiple consanguineous PCD families was identified from Kuwaiti patients and genomic DNA from the family members was isolated using standard procedures. The DNA samples from all affected individuals were analyzed by whole exome sequencing (WES). Transmission electron microscopy (TEM) and immunofluorescent analysis (IF) were performed on samples obtained by nasal brushings to identify specific structural abnormalities within ciliated cells. Results: Here, we present six multiplex families with 11 patients who all presented with typical PCD symptoms. Ten out of eleven patients inherited a 3 bp homozygous deletion of GAA in RSPH9, whereas the eleventh patients inherited this variant in trans with a frameshift deletion in RSPH9. Genetic results were confirmed by segregation analysis. The in-frame deletion of GAA in RSPH9 has previously been published as pathogenic in both annotated RSPH9 transcript variants (1 and 2). In contrast, the previously unpublished RSPH9 frameshift deletion identified in KU-15.IV2 impacts only RSPH9 transcript variant two. Regarding all 11 PCD individuals analyzed, IF results demonstrated absence of RSPH9 protein and TEM analysis showed the typical findings in RSPH9 mutant individuals. Conclusions: We present the largest cohort of PCD individuals affected by the founder in-frame deletion GAA in RSPH9. This founder variant is the most common PCD-causing variant in Bedouin Arabs in Kuwait.
Cardiac Post-Chest Radiotherapy Complications in a 50-Year-Old Patient with Hodgkin Lymphoma
Authors: Klotzka Aneta , Sobańska Karolina , Iwańczyk Sylwia , Grygier Marek , Woźniak Patrycja , Błaszyk Maciej , Rozwadowska Natalia , Lesiak Maciej
Published: October 2023

Lymphomas are a group of malignant tumors that originate in the lymphatic system. It is the most common type of blood cancer. It affects the lymph nodes, spleen, bone marrow, blood, and other organs. They can be aggressive or chronic. Hodgkin lymphoma survival rate is 2 in 100,000 people. Young adults aged 20–30 and people over 50 are most often affected. The prognosis of Hodgkin’s lymphoma is good, with a survival rate of up to 80 percent. Nevertheless, in 20–30 percent of patients who initially respond to treatment, the disease has a tendency to progress. The positive effect of radiotherapy (RT) on patients’ survival rates has been proven in many randomized clinical trials. Although the dose of chest RT has significantly reduced over the years, we still struggle with the long-term complications of post-RT repercussions, mainly because there is no established safe dose of RT affecting the heart. Other complications include earlier onset of coronary artery disease, early and late onset of pericarditis, valve degeneration (predominantly of the left heart), calcification of the aorta and its branches, heart failure, and arrhythmias. One patient can manifest each of the abovementioned complications, as in the present case. That is why choosing the right treatment strategy is crucial.
Relationship between Cervical Instability in the Course of Rheumatoid Arthritis and Pelvic Parameters of Sagittal Balance
Authors: Wróblewski Robert , Koszela Kamil , Mańczak Małgorzata , Sudoł-Szopińska Iwona , Gasik Robert
Published: October 2023

Background: The aim of the study is to search for a relationship between cervical instability in the course of rheumatoid arthritis (RA) and pelvic parameters of sagittal balance and lumbar lordosis (LL). Methods: The study included 47 patients with rheumatoid arthritis with instability of the cervical segment, who were referred for a consultation to assess indications for surgical treatment. The patients underwent a radiological functional examination of the cervical region and postural examination of the entire spine. The basic parameters of the lumbar section and pelvis of these patients were compared with the population values. Then, using statistical methods, the correlation between radiological parameters of various types of instability and lumbar lordosis (LL), pelvic parameters (PI, SS, PT), as well as the patient’s age and duration of the disease was assessed. Results: A statistical correlation was found between the instability in the cervical spine C2–C3 level and the value of the PT and PI angles. A statistically significant correlation was discovered between C1–C2 instability and younger patient age. There was no significant difference in the values of the pelvic parameters and lumbar lordosis in patients with rheumatoid arthritis compared to the population values. Conclusions: Preliminary results suggest that there is a relationship between selected pelvic parameters and the development of instability in patients with rheumatoid arthritis. This may be important in planning treatment and assessing disease progression. Further studies on a larger group of patients are needed, as well as studies evaluating the correlation between other sagittal balance parameters and cervical instability in patients with RA.
Exploring the Genetic Causes for Postnatal Growth Failure in Children Born Non-Small for Gestational Age
Authors: Kim Yoo-Mi , Lim Han-Hyuk , Kim Eunhee , Kim Geena , Kim Minji , So Hyejin , Lee Byoung Kook , Kwon Yoowon , Min Jeesu , Lee Young Seok
Published: October 2023

The most common causes of short stature (SS) in children are familial short stature (FSS) and idiopathic short stature (ISS). Recently, growth plate dysfunction has been recognized as the genetic cause of FSS or ISS. The aim of this study was to investigate monogenic growth failure in patients with ISS and FSS. Targeted exome sequencing was performed in patients categorized as ISS or FSS and the subsequent response to growth hormone (GH) therapy was analyzed. We found 17 genetic causes involving 12 genes (NPR2, IHH, BBS1, COL1A1, COL2A1, TRPS1, MASP1, SPRED1, PTPTN11, ADNP, NADSYN1, and CERT1) and 2 copy number variants. A genetic cause was found in 45.5% and 35.7% of patients with FSS and ISS, respectively. The genetic yield in patients with syndromic and non-syndromic SS was 90% and 23.1%, respectively. In the 11 genetically confirmed patients, a gain in height from −2.6 to −1.3 standard deviations after 2 years of GH treatment was found. The overall diagnostic yield in this study was 41.7%. We identified several genetic causes involving paracrine signaling, the extracellular matrix, and basic intracellular processes. Identification of the causative gene may provide prognostic evidence for the use of GH therapy in non-SGA children.
Neuroendocrine Carcinoma of the Urinary Bladder: CT Findings and Radiomics Signature
Authors: Coppola Andrea , Gatta Tonia , Pini Giacomo Maria , Scordi Giorgia , Fontana Federico , Piacentino Filippo , Minici Roberto , Laganà Domenico , Basile Antonio , Dehò Federico , Carcano Giulio , Franzi Francesca , Uccella Silvia , Sessa Fausto , Venturini Massimo
Published: October 2023

Background: We present a case series of Neuroendocrine Carcinoma of the Urinary Bladder (NECB) to analyse their radiologic appearance on CT, find a “Radiomic signature”, and review the current literature. Methods: 14 CT cases of NECB were reviewed and compared with a control group of 42 patients with high-grade non-neuroendocrine bladder neoplasm for the following parameters: ring enhancement; implantation site; dimensions; density; margins; central necrosis; calcifications; number of lesions; wall thickness; depth of invasion in the soft tissue; invasion of fat tissue; invasion of adjacent organs; lymph-node involvement; abdominal organ metastasis. To extract radiomic features, volumes of interest of bladder lesions were manually delineated on the portal-venous phase. The radiomic features of the two groups were identified and compared. Results: Statistical differences among NECB and control group were found in the prevalence of male sex (100% vs. 69.0%), hydronephrosis (71.4% vs. 33.3%), mean density of the mass (51.01 ± 15.48 vs. 76.27 ± 22.26 HU); product of the maximum diameters on the axial plane (38.1 ± 59.3 vs. 14.44 ± 12.98 cm2) in the control group, trigonal region involvement (78.57% vs. 19.05%). About the radiomic features, Student’s t-test showed significant correlation for the variables: “DependenceNonUniformity” (p: 0.048), “JointAverage” (p: 0.013), “LargeAreaLowGrayLevelEmphasis” (p: 0.014), “Maximum2DDiameterColumn” (p: 0.04), “Maximum 2DDiameterSlice” (p: 0.007), “MeanAbsoluteDeviation” (p: 0.021), “BoundingBoxA” (p: 0.022) and “CenterOfMassB” (p: 0.007). Conclusions: There is a typical pattern (male patient, large mass, trigonal area involvement) of NECB presentation on contrast-enhanced CT. Certain morphological characteristics and encouraging results about Radiomic features can help define the diagnosis.
The Urinary Concentrations of Neutrophil Gelatinase-Associated Lipocalin, Cystatin C and Osteopontin in the Healthy Term and Stable Preterm Neonates: A Pilot Study
Authors: Kamianowska Monika , Kamianowska Aleksandra , Maciejczyk Mateusz , Kurowska Anna , Błażewicz Bożena , Rogowska Agnieszka Maria , Wasilewska Anna
Published: October 2023

Background: In neonates, the assessment of kidney function with serum creatinine is limited; therefore, more effective biomarkers are needed. Aim: The study aimed at analyzing the concentrations of renal biomarkers (osteopontin, cystatin C, and NGAL) in neonates. Material and Methods: The study included 80 term and 20 preterm neonates aged 28–33 weeks of gestation. Biomarkers were measured in urine. Term neonates’ urine was collected on the 1st day of life. Preterm neonates’ urine was collected on the 1st, 8th, 15th, 22nd day of life. Biomarkers’ concentrations were normalized to urinary creatinine (cr.) and presented as urinary biomarker/cr. ratios. Results: Median values of biomarker/creatine ratios in term and preterm neonates were the following: cystatin C/cr.: 7.26 and 439.49; osteopontin/cr.: 135.86 and 1633.37; NGAL/cr. in girls: 212.14 and 256.93; and NGAL/cr. in boys 27.123 and 65.29 ng/mg cr. In preterm neonates the cystatin C/cr. ratio was higher on the 1st than on the 8th day. The osteopontin/cr. ratio did not differ between the days. The NGAL/cr. ratio in girls was higher on the 8th than on the 22nd day, and in boys, the lowest was on the 22nd day. Conclusions: Prematurity in stable, Caucasian neonates might cause higher osteopontin and cystatin C excretion, but not NGAL. The excretion of NGAL and cystatin C, but not osteopontin, may change during first weeks of premature neonate’s life.
Evaluation of Arterial Stiffness Parameters and the Growth Differentiation Factor-15 Level in Patients with Premature Myocardial Infarction
Authors: Dogan Zekeriya , Ileri Cigdem , Kay Esin A. , Sunbul Murat , Gurel Emre Y. , Özben Sadıc Beste , Sayar Nurten , Ergun Tulin , Tigen Kursat M.
Published: October 2023

Background: Myocardial infarction (MI) is increasing at a younger age. Growth differentiation factor-15 (GDF-15) has been implicated in several key mechanisms of atherogenesis. Arterial stiffness parameters, including pulse wave velocity (PWV) and the augmentation index (AIx), can indicate the presence or progression of atherosclerosis. The aim of this study is to evaluate the GDF-15 level and arterial stiffness parameters in patients with premature MI. Method: Thirty patients aged ≤45 years (mean age: 39 ± 5 years, 23 male) who recovered from a MI and 15 age and sex-matched subjects were consecutively included. The serum GDF-15 concentration levels and arterial stiffness parameters of the patients and controls were measured. Results: GDF-15 levels were significantly higher in patients with premature MI, while there were no significant differences in PWV and AIx between the groups. The GDF-15 level was correlated negatively with high-density lipoprotein (HDL) cholesterol and positively with uric acid levels. Both GDF-15 (p = 0.046, odds ratio: 1.092, 95% confidence interval: 1.003–1.196) and HDL cholesterol (p = 0.037, odds ratio: 0.925, 95% confidence interval: 0.859–0.995) were found as independent factors associated with premature MI. Conclusions: GDF-15 could be a risk factor for premature MI. Further studies are needed to elucidate the central role of GDF-15 in the pathophysiology of early atherosclerosis and MI in the young population.
Self-Configuration Management towards Fix-Distributed Byzantine Sensors for Clustering Schemes in Wireless Sensor Networks
Authors: Elsayed Walaa M. , El-Shafeiy Engy , Elhoseny Mohamed , Hassan Mohammed K.
Published: October 2023

To avoid overloading a network, it is critical to continuously monitor the natural environment and disseminate data streams in synchronization. Based on self-maintaining technology, this study presents a technique called self-configuration management (SCM). The purpose is to ensure consistency in the performance, functionality, and physical attributes of a wireless sensor network (WSN) over its lifetime. During device communication, the SCM approach delivers an operational software package for the radio board of system problematic nodes. We offered two techniques to help cluster heads manage autonomous configuration. First, we created a separate capability to determine which defective devices require the operating system (OS) replica. The software package was then delivered from the head node to the network’s malfunctioning device via communication roles. Second, we built an autonomous capability to automatically install software packages and arrange the time. The simulations revealed that the suggested technique was quick in transfers and used less energy. It also provided better coverage of system fault peaks than competitors. We used the proposed SCM approach to distribute homogenous sensor networks, and it increased system fault tolerance to 93.2%.
From Tick to Test: A Comprehensive Review of Tick-Borne Disease Diagnostics and Surveillance Methods in the United States
Authors: Rowan Sean , Mohseni Nazleen , Chang Mariann , Burger Hannah , Peters Mykah , Mir Sheema
Published: October 2023

Tick-borne diseases (TBDs) have become a significant public health concern in the United States over the past few decades. The increasing incidence and geographical spread of these diseases have prompted the implementation of robust surveillance systems to monitor their prevalence, distribution, and impact on human health. This comprehensive review describes key disease features with the geographical distribution of all known tick-borne pathogens in the United States, along with examining disease surveillance efforts, focusing on strategies, challenges, and advancements. Surveillance methods include passive and active surveillance, laboratory-based surveillance, sentinel surveillance, and a One Health approach. Key surveillance systems, such as the National Notifiable Diseases Surveillance System (NNDSS), TickNET, and the Tick-Borne Disease Laboratory Network (TBDLN), are discussed. Data collection and reporting challenges, such as underreporting and misdiagnosis, are highlighted. The review addresses challenges, including lack of standardization, surveillance in non-human hosts, and data integration. Innovations encompass molecular techniques, syndromic surveillance, and tick surveillance programs. Implications for public health cover prevention strategies, early detection, treatment, and public education. Future directions emphasize enhanced surveillance networks, integrated vector management, research priorities, and policy implications. This review enhances understanding of TBD surveillance, aiding in informed decision-making for effective disease prevention and control. By understanding the current surveillance landscape, public health officials, researchers, and policymakers can make informed decisions to mitigate the burden of (TBDs).
Changes in Diversity of Silica-Scaled Chrysophytes during Lake–River–Reservoir Transition (Baikal–Angara–Irkutsk Reservoir)
Authors: Bessudova Anna , Galachyants Yuri , Firsova Alena , Hilkhanova Diana , Nalimova Maria , Marchenkov Artyom , Mikhailov Ivan , Sakirko Maria , Likhoshway Yelena
Published: October 2023

Hydroelectric dams create new ecosystems such as reservoirs. Several hydroelectric dams forming shallow reservoirs were built on the Angara River flowing out of Lake Baikal. The first of them in downstream Angara is Irkutsk Reservoir, with several shallow bays. Since silica-scaled chrysophytes are effective bioindicators for aquatic ecosystems, this paper aimed to determine their distribution, taxonomic structure and species richness in South Baikal and Irkutsk Reservoir, which have different environmental parameters. Thirty-one species were found using scanning and transmission electron microscopy. Only seven of them inhabited South Baikal in June 2023 at 3.66–4.51 °C and pH 7.80–8.24, with Chrysosphaerella baicalensis, Spiniferomonas trioralis f. cuspidata and Mallomonas alpina being prevalent. Only one species (M. alpina) was dominant in Irkutsk Reservoir at all stations at a water temperature of 5.33–11.55 °C and pH 8.10–8.52, alongside three other abundant species, Synura cf. glabra, Mallomonas acaroides and M. crassisquama. The maximum number of species (23) was found in a shallow bay of the reservoir at maximal values of temperature (11.5 °C) and pH (8.57) and minimal values of phosphate and nitrate concentrations during the study. The enrichment of Irkutsk Reservoir in species of silica-scaled chrysophytes was due both to cosmopolitan widespread and polyzonal species as well as to rare boreal, arctic–boreal, and unknown, possibly new species.
Research on Loaded Contact Analysis and Tooth Wear Calculation Method of Cycloid–Pin Gear Reducer
Authors: Wang Yongqiang , Wei Bingyang , Wang Zhen , Yang Jianjun , Xu Jiake
Published: October 2023

This study establishes the geometric model of cycloid–pin gear meshing transmission based on the multi-tooth meshing characteristics of the cycloid speed reducer. The calculation and analysis of meshing motion parameters of the cycloid speed reducer are carried out. An integrated calculation flow is presented for solving the question of the loaded tooth contact of the cycloid speed reducer by using the elimination clearance method of gradual contact and the quasi-Hertz contact simulation of the tooth surface under loads. The loaded transmission error is obtained, and both the number of pins participating in the meshing and the contact area of tooth surfaces are determined. Using the regression formula of the wear coefficient, the dynamic wear coefficient is quickly solved on the instantaneous contact line of the tooth surface. Thereby, the wear distribution law of two tooth surfaces appears. The results show that there is a singular point in the wear of the pin teeth, with a maximum wear of 100 μm, that seriously affects the meshing accuracy of the tooth surface and thus affects the accuracy and lifespan of the reducer.
Exploring the Potential of Alternative Materials in Concrete Mixtures: Effect of Copper Slag on Mechanical Properties and Carbonation Resistance
Authors: Silva Yimmy Fernando , Villaquirán-Caicedo Mónica , Izquierdo Silvia
Published: October 2023

In this study, the effect on the flowability, compressive strength, absorption, sorptivity, and carbonation resistance of concrete with different copper slag (CS) replacement ratios was investigated. For this research, four concrete mixes with different percentages of CS were made (0%, 10%, 20%, and 30% of CS as replacement of cement by volume). In addition, the microstructure was analyzed by X-ray diffraction (XRD), scanning electron microscope (SEM), and thermogravimetric analysis (TG–DTG). The results shows that the incorporation of CS reduces the workability and compressive strength of the mixtures, being more significant in concrete with 30% CS. The carbonation depth of concrete with CS increases monotonically with increasing CS. In addition, the compressive strength of the carbonated (20% and 30% CS) concretes show a loss of compressive strength at 90 days of exposure when compared to their water-cured counterparts. The use of low percentages of CS does not generate a decrease in workability and its mechanical effect is not significant at prolonged ages, so the use of this waste as SCM in percentages close to 10% is a viable alternative to the sustainability of concrete and the management of this residue.
Impact of Mn Alloying on Phase Stabilities, Magnetic Properties and Electronic Structures in Fe
Authors: Yang Hao , Yang Jin-Han , Zhao Ying , Ma Han , Tian Yanzhong , Cai Minghui , Tang Shuai , Liu Yandong , Zhao Xiang , Yan Hai-Le , Zuo Liang
Published: October 2023

Impacts of Mn alloying on lattice stabilities, magnetic properties, electronic structures of the bcc and fcc phases and the fcc→bcc phase transition in Fe16−xMnx (x = 0, 1 and 2) alloys are studied by first-principles calculations. Results show that the doped Mn atom prefers ferromagnetic and antiferromagnetic interaction with the host Fe atoms in the bcc and fcc phases, respectively. In these two phases, the magnetic moment of Mn is smaller and larger than Fe, respectively. The local moment of Fe is decided by the Fe-Mn distance in the bcc phase, whereas in the fcc phase, it is determined by spatial orientation with Mn. In the different phases, Mn prefers different site occupations, which can be understood from the electronic density of states near Fermi energy, implying a possibility of element redistribution during phase transition. The driving force of phase transition decreases with Mn alloying. Both destabilized bcc phase and stabilized fcc phase contribute to the inhibited phase transition, but the latter plays a dominant role. Antiferromagnetism is recognized as the key reason for the enhanced stability of the fcc phase by Mn alloying.
The Influence of Heat Treatment Temperature on Tensile Properties of Metal-Bonded Diamond Composites Fabricated via Selective Laser Melting
Authors: Han Guangyao , Xu Yangli , Wei Jinquan , Huang Guoqin , Li Tingting , He Yiqiang , Xie Zhiping , Mai Zihong , Yang Zeling
Published: October 2023

Selective Laser Melting (SLM) is an effective technology for fabricating new types of porous metal-bonded diamond tools with complex geometries. However, due to the high cooling rate and internal stresses during SLM fabrication, defects such as high porosities and interface gaps still need to be resolved before it can be considered for use in other applications. The influence of heat treatment temperature on internal characterization, interface microstructures, and tensile properties of AlSi7Mg-bonded diamond composites fabricated by SLM were investigated in this work. From experimental results, the porosities of HT-200, HT-350, and HT-500 specimens were 12.19%, 11.37%, and 11.14%, respectively, showing a slightly lower percentage than that of the No-HT specimen (13.34%). Here, HT represents “Heat Treatment”. For No-HT specimens, an obvious un-bonding area can be seen in the interface between AlSi7Mg and diamond, whereas a relative closer interface can be observed for HT-500 specimens. After heat treatment, the elastic modulus of specimens showed a relative stable value (16.77 ± 2.79~18.23 ± 1.72 GPa), while the value of yield strength decreased from 97.24 ± 4.48 to 44.94 ± 7.06 MPa and the value of elongation increased from 1.98 ± 0.05 to 6.62 ± 0.51%. This difference can be attributed mainly to the disappearance of the solid-solution hardening effect due to the increase of Si content after heat treatment.
A Hyperbolic Secant-Squared Distribution via the Nonlinear Evolution Equation and Its Application
Authors: Daghistani Amira F. , Abd El-Bar Ahmed M. T. , Gemeay Ahmed M. , Abdelrahman Mahmoud A. E. , Hassan Samia Z.
Published: October 2023

In this article, we present a hyperbolic secant-squared distribution via the nonlinear evolution equation. Namely, for this equation, the probability density function of the hyperbolic secant-squared (HSS) distribution has been determined. The density of our model has a variety of shapes, including symmetric, left-skewed, and right-skewed. Eight distinct frequent list estimation methods have been proposed for estimating the parameters of our models. Additionally, these estimation techniques have been used to examine the behavior of the HSS model parameters using data sets that were generated randomly. To demonstrate how the findings may be used to model real data using the HSS distribution, we also use real data. Finally, the proposed justification can be applied to a variety of other complex physical models.
Research on Intelligent Control Method of Launch Vehicle Landing Based on Deep Reinforcement Learning
Authors: Xue Shuai , Bai Hongyang , Zhao Daxiang , Zhou Junyan
Published: October 2023

A launch vehicle needs to adapt to a complex flight environment during flight, and traditional guidance and control algorithms can hardly deal with multi-factor uncertainties due to the high dependency on control models. To solve this problem, this paper designs a new intelligent flight control method for a rocket based on the deep reinforcement learning algorithm driven by knowledge and data. In this process, the Markov decision process of the rocket landing section is established by designing a reinforcement function with consideration of the combination effect on the return of the terminal constraint of the launch vehicle and the cumulative return of the flight process of the rocket. Meanwhile, to improve the training speed of the landing process of the launch vehicle and to enhance the generalization ability of the model, the strategic neural network model is obtained and trained via the form of a long short-term memory (LSTM) network combined with a full connection layer as a landing guidance strategy network. The proximal policy optimization (PPO) is the training algorithm of reinforcement learning network parameters combined with behavioral cloning (BC) as the reinforcement learning pre-training imitation learning algorithm. Notably, the rocket-borne environment is transplanted to the Nvidia Jetson TX2 embedded platform for the comparative testing and verification of this intelligent model, which is then used to generate real-time control commands for guiding the actual flying and landing process of the rocket. Further, comparisons of the results obtained from convex landing optimization and the proposed method in this work are performed to prove the effectiveness of this proposed method. The simulation results show that the intelligent control method in this work can meet the landing accuracy requirements of the launch vehicle with a fast convergence speed of 84 steps, and the decision time is only 2.5 ms. Additionally, it has the ability of online autonomous decision making as deployed on the embedded platform.
Maps Preserving Zero ∗-Products on ℬ(ℋ)
Authors: Wang Meili , Zhang Jing , Li Yipeng , Shangguan Lina
Published: October 2023

The conventional research topic in operator algebras involves exploring the structure of algebras and using homomorphic mappings to study the classification of algebras. In this study, a new invariant is developed based on the characteristics of the operator using the linear preserving method. The results show that the isomorphic mapping is used for preserving this invariant, which provides the classification information of operator algebra from a new perspective. Let H and K be Hilbert spaces with dimensions greater than two, and let B(H) and B(K) be the set of all bounded linear operators on H and K, respectively. For A,B∈B(H), the ∗, ∗-Lie, and ∗-Jordan products are defined by A∗B, A∗B−B∗A, and A∗B+B∗A, respectively. Let Φ:B(H)→B(K) be an additive unital surjective map. It is confirmed that if Φ preserves zero ∗, ∗-Lie, and ∗-Jordan products, then Φ is unitary or conjugate unitary isomorphisms.
Phenolic Acid Functional Quaternized Chitooligosaccharide Derivatives: Preparation, Characterization, Antioxidant, Antibacterial, and Antifungal Activity
Authors: Sun Yan , Cui Jingmin , Tian Liguang , Mi Yingqi , Guo Zhanyong
Published: October 2023

As a promising biological material, chitooligosaccharide (COS) has attracted increasing attention because of its unique biological activities. In this study, fourteen novel phenolic acid functional COS derivatives were successfully prepared using two facile methods. The structures of derivatives were characterized by FT-IR and 1H NMR spectra. The in vitro antioxidant activity experiment results demonstrated that the derivatives presented stronger 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), superoxide, hydroxyl radical scavenging activity and reducing power, especially the N,N,N-trimethylated chitooligosaccharide gallic acid salt (GLTMC), gallic acid esterified N,N,N-trimethylated chitooligosaccharide (GL-TMC) and caffeic acid N,N,N-trimethylated chitooligosaccharide (CFTMC) derivatives. Furthermore, the antifungal assay was carried out and the results indicated that the salicylic acid esterified N,N,N-trimethylated chitooligosaccharide (SY-TMC) had much better inhibitory activity against Botrytis cinerea and Fusarium graminearum. Additionally, the results of the bacteriostasis experiment showed that the caffeic acid esterified N,N,N-trimethylated chitooligosaccharide (CF-TMC) had the potential ability to inhibit Escherichia coli and Staphylococcus aureus bacteria. Altogether, this study may provide a neoteric method to produce COS derivatives with significantly increased biological activities, which have potential use in food, medicine, and health care products and other related industries.