bluuebunny/crossref_metadata_2025 huggingface dataset:
Relatively Low Light Intensity Promotes Phosphorus Absorption and Enhances the Ethylene Signaling Component EIN3 in Maize, Wheat, and Oilseed Rape
Authors: Zhang Meng-Yao , Cai Xin , Wan Yu-Ting , Fu Yu-Fan , Yang Xin-Yue , Zhang Zhong-Wei , Yuan Shu
Published: February 2022

Previous studies have found that once seedlings break the soil, light can induce the degradation of the key ethylene signaling element ethylene insensitive 3 (EIN3), so as to indirectly inhibit the synthesis of ethylene. Ethylene is the most important hormone in phosphorus absorption by plants, which induces the expression of acid phosphatase (APase) and phosphorus starvation response genes. Therefore, it might be speculated that changes in light intensity could regulate phosphorus absorption to some degree. However, there are few reports on the mechanism by which light intensity regulates phosphorus metabolism. In this study, the effects of different light intensities on phosphorus assimilation and metabolism in plants were studied. The results showed that relatively low light intensity could promote the secretion of APase, so as to increase the concentration of plant total phosphorus and cellular Pi. However, the low light intensity may also inhibit plant growth. Among the three species, oilseed rape was the most sensitive to the low light intensity. The steady-state level of the EIN3 protein decreased significantly under a relatively high light intensity; while the ethylene level also decreased under the high light intensity. Therefore, appropriate reductions in light intensity may simultaneously promote phosphorus assimilation and maintain plant growth.
Grain Yield, Nitrogen Use Efficiency and Antioxidant Enzymes of Rice under Different Fertilizer N Inputs and Planting Density
Authors: Wang Wenxi , Shen Congcong , Xu Qin , Zafar Sundus , Du Bin , Xing Danying
Published: February 2022

Nitrogen fertilizer and planting density are key factors affecting rice yield and nitrogen utilization efficiency. There is still a need to optimize nitrogen fertilizer and planting density management for high yield. We studied the effects of different quantities of nitrogen application (N0 0 kg ha−1, N1 120 kg ha−1, N2 180 kg ha−1) and planting density (low-density: 18.8 hills m−2; high-density: 37.5 hills m−2) on rice yield, photosynthetic characteristics, antioxidant system, and nitrogen use efficiency. ANOVA results indicated that most tested traits were affected by environment, planting density, N application, and their interactions. Comparing the results of low-density planting, high-density planting increased the panicle number by 21.12% but decreased the grain number per panicle and yield by 3.97% and 22.48%, respectively. Similarly, the superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) activities and PFPn (partial factor productivity of nitrogen) decreased by 8.20%, 6.99%, 16.41%, 14.28%, and 5.73%, respectively, while HIn (N harvest index) increased by 1.32%. Compared to no N application, N application treatments increased the panicle number, grain number per panicle, and yield by 9.92%, 17.64%, and 37.83% in the N1 treatment; and increased by 17.15%, 29.09%, and 128.94% in the N2 treatment, respectively. N application significantly increased net photosynthetic rates and enzyme activities of antioxidant antioxidases. Compared with N1, N2 decreased REn (apparent recovery efficiency of N), AEn (agronomic N use efficiency), and PFPn by 8.98%, 11.80%, and 25.14%, respectively, while, compared with N0, N1 increased HIn by 8.50%. It was observed that nitrogen fertilizer and planting density had an interaction effect on the net photosynthetic rate, antioxidant enzyme activities, and PFPn and HIn. Given a comprehensive consideration, it is best to apply nitrogen at a rate of 120 kg ha−1 at the planting density of 37.5 hills m−2 for high grain yield and high N use efficiency in our experimental site.
Deciphering Bacterial Community of the Fallow and Paddy Soil Focusing on Possible Biocontrol Agents
Authors: Abbas Aqleem , Duan Jie , Abdoulaye Assane Hamidou , Fu Yanping , Lin Yang , Xie Jiatao , Cheng Jiasen , Jiang Daohong
Published: February 2022

In pursuing higher rice production, we have often jeopardized soil at an alarming rate. It is hypothesized that intensive farming practices degrade soil health and increase the abundance of rice diseases while fallowing increases the abundance of biocontrol agents. In this study, the bacterial community was monitored in the paddy and fallow soil. Proteobacteria, Bacteroidetes, and Actinobacteria were abundant in the fallow soil, whereas Acidobacteria, Chloroflexi, and Gemmatimonadetes were more abundant in the paddy soil. Among the potential biocontrol agents, Bacillus, Thiobacillus, Rhizobium, Massilia, Rhizobacter, Streptomyces, Micromonospora, and Pseudonocardia were more abundant in the fallow soil, while Pseudomonas and Burkholderia were more abundant in the paddy soil. The possible rice pathogens, i.e., Xanthomonas and Erwinia, were more abundant in the paddy soil. The alpha diversity was higher in paddy soil than in fallow soil. Additionally, the principal coordinate analysis based on UniFrac distances revealed distinct clusters of the soils. Moreover, the functional analysis suggested that the fallow soil was abundant in genes associated with the biosynthesis of siderophores and secondary metabolites. In contrast, the paddy soil was abundant in genes related to plant-pathogen interactions. In conclusion, these results highlight the significance of fallowing to improve soil health.
Benefits of Crop Rotation on Climate Resilience and Its Prospects in China
Authors: Yu Taize , Mahe Leo , Li Ying , Wei Xue , Deng Xiaoshang , Zhang Dan
Published: February 2022

In the context of climate change, increases in extreme weather have caused a series of problems, severely reduced crop yield, and caused a loss of agricultural cultivation. In addition, because of the high economic benefits, continuous cropping has become more popular but it leads to higher land-use intensity in production systems, aggravating the problems of extreme climate and seriously influencing China’s agricultural production and ecological environment. From this, the importance of improvements to cropping systems’ resilience to climate change is now much clearer than before. Crop rotation is an important tool for improving the climate resilience of the agricultural production system and effectively solving the shortcomings of the current continuous crop methodology. Crop rotation is indispensable in many national strategies, including food security, ecological environment development, and rural revitalization. This study aimed to promote the improvement of the crop rotation system in China and aimed to play a significant role in guiding China towards the large-scale development of crop rotation. This literature review shows that crop rotation can effectively enhance climate resilience and reduce the fragility of agricultural cropping systems. It then delves into the origin and development of crop rotation, and summarizes the characteristics of crop rotation. In view of the neglect of ecological benefits in China’s agricultural development, this article puts forward three suggestions: first, developing crop rotation technology based on local conditions; second, paying attention to the ecological benefits of crop rotation subsidies, followed by implementing appropriate and flexible subsidy policies; and, finally, carrying out rational evaluations and policy adjustment of crop rotation practices.
Seed Size, Planting Depth, and a Perennial Groundcover System Effect on Corn Emergence and Grain Yield
Authors: Kimmelshue Chad L. , Goggi Susana , Moore Kenneth J.
Published: February 2022

The intensive corn (Zea mays L.) and soybean (Glycine max (L.) Merr.) production practices currently used in the Midwestern U.S. concern producers and stakeholders. The negative impact of these two-crop rotations on the environment affects water quality and soil erosion and increases flooding risks. Due to these concerns, cover crops and, specifically, perennial groundcover (PGC) cropping systems have gained greater interest. These perennial species have growing patterns compatible with corn and soybean, and can help rebuild the ecosystem while maintaining good cash crop yields. In addition, producers also are interested in the possible effect of seed size and planting depth on uneven emergence in corn. The successful adoption of PGC systems ultimately depends on the successful corn seedling emergence and consistent yield. The objective of the study was to understand the effects of seed characteristics and placement on emergence, grain yield, and grain quality in corn planted using a Kentucky bluegrass (Poa pratensis L.) (KBG)-PGC and a bare-soil cropping system and to determine grain quality attributes and grain moisture dry-down in a PGC field when compared to a conventional cropping system. Commercially-sized seed and seed sized in the laboratory to represent a narrower seed size distribution were planted in KBG-PGC and bare soil systems at two planting depths (3.18 and 6.35 cm). The two-year experiments were planted in a split-plot RCB design with four replications. Individual plants were flagged at emergence, and ears from each plant were harvested individually. Separating the seed lot into different size distributions did not affect seed germination under ideal (standard germination and speed of germination tests) or stressful (cold test) conditions. Seed size distribution also did not influence emergence rate and yield in a conventional tillage (bare soils) or KBG-PGC system. These results indicate that seed sizing specifications and seed size cutoffs currently used by seed companies are suitable for uniform emergence and maximum grain yield in both cropping systems. Seed placement was crucial to uniform emergence in both cropping systems, while seed size distribution did not play a role in emergence for either system. The PGC cropping system delayed seed corn emergence and reduced grain yields as much as 50%. This information is important for those producers considering adopting a PGC system because it demonstrates that uniform planting depth is more important than seed size distribution.
The Prevalence and Molecular Biology of Staphylococcus aureus Isolated from Healthy and Diseased Equine Eyes in Egypt
Authors: Tahoun Amin , Elnafarawy Helmy K. , El-Sharkawy Hanem , Rizk Amira M. , Alorabi Mohammed , El-Shehawi Ahmed M. , Youssef Mohamed A. , Ibrahim Hussam M. M. , El-Khodery Sabry
Published: February 2022

This work aimed to characterize S. aureus isolates from the eyes of healthy and clinically affected equines in the Kafrelsheikh Governorate, Egypt. A total of 110 animals were examined for the presence of S. aureus, which was isolated from 33 animals with ophthalmic lesions and 77 healthy animals. We also investigated the antimicrobial resistance profile, oxacillin resistance mechanism, and the major virulence factors implicated in many studies of the ocular pathology of pathogenic S. aureus. The association between S. aureus eye infections and potential risk factors was also investigated. The frequency of S. aureus isolates from clinically affected equine eyes was significantly higher than in clinically healthy equids. A significant association was found between the frequency of S. aureus isolation from clinically affected equine eyes and risk factors including age and season but not with sex or breed factors. Antimicrobial resistance to common antibiotics used to treat equine eyes was also tested. Overall, the isolates showed the highest sensitivity to sulfamethoxazole (100%) and the highest resistance to cephalosporin (90.67%) and oxacillin (90.48%). PCR was used to demonstrate that mecA was present in 100% of oxacillin- and β-lactam-resistant S. aureus strains. The virulence factor genes Spa (x region), nuc, and hlg were identified in 62.5%, 100%, and 56%, of isolates, respectively, from clinically affected equines eyes. The severity of the eye lesions increased in the presence of γ-toxin-positive S. aureus. The phylogenetic tree of the Spa (x region) gene indicated a relationship with human reference strains isolated from Egypt as well as isolates from equines in Iran and Japan. This study provides insight into the prevalence, potential risk factors, clinical pictures, zoonotic potential, antimicrobial resistance, and β-lactam resistance mechanism of S. aureus strains that cause eye infection in equines from Egypt.
Antioxidant Activity of Natural Hydroquinones
Authors: Giner Rosa M. , Ríos José Luis , Máñez Salvador
Published: February 2022

Secondary metabolites derived from hydroquinone are quite rare in nature despite the original simplicity of its structure, especially when compared to other derivatives with which it shares biosynthetic pathways. However, its presence in a prenylated form is somewhat relevant, especially in the marine environment, where it is found in different algae and invertebrates. Sometimes, more complex molecules have also been identified, as in the case of polycyclic diterpenes, such as those possessing an abietane skeleton. In every case, the presence of the dihydroxy group in the para position gives them antioxidant capacity, through its transformation into para-quinones.This review focuses on natural hydroquinones with antioxidant properties referenced in the last fifteen years. This activity, which has been generally demonstrated in vitro, should lead to relevant pharmacological properties, through its interaction with enzymes, transcription factors and other proteins, which may be particularly relevant for the prevention of degenerative diseases of the central nervous system, or also in cancer and metabolic or immune diseases. As a conclusion, this review has updated the pharmacological potential of hydroquinone derivatives, despite the fact that only a small number of molecules are known as active principles in established medicinal plants. The highlights of the present review are as follows: (a) sesquiterpenoid zonarol and analogs, whose activity is based on the stimulation of the Nrf2/ARE pathway, have a neuroprotective effect; (b) the research on pestalotioquinol and analogs (aromatic ene-ynes) in the pharmacology of atherosclerosis is of great value, due to their agonistic interaction with LXRα; and (c) prenylhydroquinones with a selective effect on tyrosine nitration or protein carbonylation may be of interest in the control of post-translational protein modifications, which usually appear in chronic inflammatory diseases.
Toward a Symbolic AI Approach to the WHO/ACSM Physical Activity & Sedentary Behavior Guidelines
Authors: Allocca Carlo , Jilali Samia , Ail Rohit , Lee Jaehun , Kim Byungho , Antonini Alessio , Motta Enrico , Schellong Julia , Stieler Lisa , Haleem Muhammad Salman , Georga Eleni , Pecchia Leandro , Gaeta Eugenio , Fico Giuseppe
Published: February 2022

The World Health Organization and the American College of Sports Medicine have released guidelines on physical activity and sedentary behavior, as part of an effort to reduce inactivity worldwide. However, to date, there is no computational model that can facilitate the integration of these recommendations into health solutions (e.g., digital coaches). In this paper, we present an operational and machine-readable model that represents and is able to reason about these guidelines. To this end, we adopted a symbolic AI approach that combines two paradigms of research in knowledge representation and reasoning: ontology and rules. Thus, we first present HeLiFit, a domain ontology implemented in OWL, which models the main entities that characterize the definition of physical activity, as defined per guidance. Then, we describe HeLiFit-Rule, a set of rules implemented in the RDFox Rule language, which can be used to represent and reason with these recommendations in concrete real-world applications. Furthermore, to ensure a high level of syntactic/semantic interoperability across different systems, our framework is also compliant with the FHIR standard. Through motivating scenarios that highlight the need for such an implementation, we finally present an evaluation of our model that provides results that are both encouraging in terms of the value of our solution and also provide a basis for future work.
Acoustic Equivalent Lasing and Coherent Perfect Absorption Based on a Conjugate Metamaterial Sphere
Authors: Ma Xing-Xing , Wei Qi , Zhu Xing-Feng , Yao Jie , Wu Da-Jian
Published: February 2022

Acoustic conjugate metamaterials (ACMs), in which the imaginary parts of the effective complex mass density and bulk compressibility are cancelled out in the refractive index, possess the elements of loss and gain simultaneously. Previous works have focused on panel ACMs for plane wave incidence. In this paper, we explore the extraordinary scattering properties, including the acoustic equivalent lasing (AEL) and coherent perfect absorption (CPA) modes, of a three-dimensional ACM sphere, where incident spherical waves with specific topological orders could be extremely scattered and totally absorbed, respectively. Theoretical analysis and numerical simulations show that the AEL or CPA mode with a single order can be realized with a small monolayer ACM sphere with appropriate parameters. A huge (relative to incident wavelength) ACM sphere with pure imaginary parameters could support the even- (or odd-) order AEL and odd- (or even-) order CPA modes simultaneously. In addition, the AEL and/or CPA with multiple orders could be realized based on a small multilayered ACM sphere. The proposed ACM sphere may provide an alternative method to design acoustic functional devices, such as amplifiers and absorbers.
Optimization of Spot Efficiency of Double-Helix Point Spread Function and Its Application in Intracellular Imaging
Authors: Yang Jun , Du Hanliang , Chai Zhenhao , Zhang Lei , Li Ben Q. , Cui Jianlei , Mei Xuesong
Published: February 2022

The nano-scale spatial positioning of nanoparticles in tumor cells can be achieved through the double-helix point spread functions (DH-PSF). Nevertheless, certain issues such as low light intensity concentration of the main lobes, the influence of the side lobes, and the aberrations of the imaging system result in poor image quality and reduce the positioning accuracy of the fluorescent nanoparticles. In this paper, an iterative optimization algorithm that combines Laguerre–Gaussian modes and Zernike polynomials is proposed. The double-helix point spread function, constructed by the linear superposition of the Laguerre–Gaussian mode and Zernike polynomials, is used to express aberrations in the imaging system. The simulation results indicated that the light intensity concentration of the main lobes is increased by 45.51% upon the use of the optimization process. Based on the simulation results, the phase modulation plate was designed and processed while a 4f positioning imaging system was built. Human osteosarcoma cells, labeled by CdTe/CdS/ZnS quantum dots, were used as samples, and the position imaging experiment was carried out. The image information entropy was used as the clarity evaluation index. The experimental results showed that the image information entropy of the DH-PSF position imaging was reduced from 4.22 before optimization to 2.65 after optimization, and the image clarity was significantly improved. This result verified the effectiveness of the optimization method that was proposed in this work.
A Two-Step Surface Reconstruction Method Using Signed Marching Cubes
Authors: Zhang Ju , Zhong Deyun , Wang Liguan
Published: February 2022

In this paper, we explore a new approach, a two-step surface reconstruction method to extract the target isosurface from a given implicit function efficiently. Our main contribution is that we improve the surface reconstruction process by accelerating the speed of evaluation using signed marching cubes. The basic strategy is to filter the invalid voxels that do not intersect with the target isosurface in a low-cost manner, and to evaluate the valid voxels that intersect with the target isosurface accurately. The improved signed marching cubes method consists of a rough evaluation step and an exact evaluation step. The coarse evaluation step evaluates the points of all voxels using the fast multipole method with a lower order. After the rough evaluation step, the voxels that intersect with the target isosurface are screened out. Then, the exact evaluation step evaluates the points of filtered voxels using the fast multipole method with a higher order. The experimental results show that, compared with the traditional marching cubes method, the improved reconstruction method reduces the amounts of calculation for invalid voxels that do not intersect with the target isosurfaces, which is useful to improve the efficiency of surface reconstruction.
A Design Method for Gammachirp Filterbank for Loudness Compensation in Hearing Aids
Authors: Guo Ruxue , Liang Ruiyu , Wang Qingyun , Zou Cairong
Published: February 2022

Because the hearing impaired often experience different degrees of hearing loss along with the loss of frequencies, the loudness compensation algorithm in hearing aids decomposes the speech signal and compensates with different frequency bands based on their audiograms. However, the speech quality of the compensated signal is unsatisfactory because the traditional filterbanks fail to fully consider the characteristics of human hearing and personalized hearing loss. In this study, an effective design for the gammachirp filterbank for the loudness compensation algorithm was proposed to improve the speech quality of hearing aids. Firstly, a multichannel gammachirp filterbank was employed to decompose the signals. Then, the adjacent bands were merged into one channel, guided by the proposed combination method. After obtaining the personalized filterbank, each band conducted a loudness compensation to match the requirements of the audiograms. The excellent advantage of the gammachirp filterbank is that it can simulate the characteristics of the basilar membrane. Furthermore, the novel channel combination method considers the information from the audiograms and the relationship between frequency ranges and speech intelligibility. The experimental results showed that the proposed multichannel gammachirp filterbank achieves better speech signal decomposition and synthesis, and good performance can be gained with fewer channels. The loudness compensation algorithm based on the gammachirp filterbank effectively improves sentence intelligibility. The sentence recognition rate of the proposed method is higher than that of a system with a gammatone filterbank by approximately 13%.
Synergetic Synthesis of Nonlinear Laws of Throttle Control of a Pneumatic Drive
Authors: Obukhova Elena , Veselov Gennady E. , Obukhov Pavel , Beskopylny Alexey , Stel’makh Sergey A. , Shcherban’ Evgenii M.
Published: February 2022

Currently, a significant trend in control in robotic systems is developing and improving linear and nonlinear control algorithms to improve the overall quality of production with high accuracy and adaptability. The present study considers a synergistic synthesis of throttle control of a pneumatic distributor valve and backpressure control for piston rod positioning. The article presents the synthesis of control laws for the position of a pneumatic cylinder piston using the method of analytical design of aggregated regulators (ADAR) of synergetic control theory (STC), which allows operation with nonlinear mathematical models, eliminating the loss of information about the object during linearization. A comparative calculation of the energy efficiency of backpressure control and throttle control methods was carried out, while the numerical value of the total airflow with throttle control is 0.0569 m3⁄s and, with backpressure control, it is 0.0337 m3⁄s. Using a P controller in a linear model gives a transient oscillatory process damped in 2–2.5 s. When using a PID controller, the process has an overshoot equal to 11.5%, while the synergistic controller allows you to smoothly move the drive stem to a given position without overshoot. The parametric uncertainty analysis of the considered mathematical model is carried out. The model’s main parameters are identified, which change the actual functioning of the system under study. The inconsistency of applying classical control laws based on typical controllers to parametrically indeterminate mathematical models is shown.
Emerging Technologies for Next-Generation Applied Science Systems
Authors: Kim Byung-Seo
Published: February 2022

In the past decade, unlike other industries, Information Technology has been substantially advanced and improved [...]
Spacecraft Telemetry Anomaly Detection Based on Parametric Causality and Double-Criteria Drift Streaming Peaks over Threshold
Authors: Zeng Zefan , Jin Guang , Xu Chi , Chen Siya , Zhang Lu
Published: February 2022

Most of the spacecraft telemetry anomaly detection methods based on statistical models suffer from the problems of high false negatives, long time consumption, and poor interpretability. Besides, complex interactions, which may determine the propagation of anomalous mode between telemetry parameters, are often ignored. To discover the complex interaction between spacecraft telemetry parameters and improve the efficiency and accuracy of anomaly detection, we propose an anomaly detection framework based on parametric causality and Double-Criteria Drift Streaming Peaks Over Threshold (DCDSPOT). We propose Normalized Effective Transfer Entropy (NETE) to reduce the error and noise caused by nonstationarity of the data in the calculation of transfer entropy, and then apply NETE to improve the Multivariate Effective Source Selection (MESS) causal inference algorithm to infer parametric causality. We define the Weighted Source Parameter (WSP) of the target parameter to be detected, then DSPOT is employed to set multi-tier thresholds for target parameter and WSP. At last, two criteria are formulated to determine anomalies. Additionally, to cut the time consumption of the DCDSPOT, we apply Probability Weighted Moments (PWM) for parameter estimation of Generalized Pareto Distribution (GPD). Experiments on real satellite telemetry dataset shows that our method has higher recall and F1-score than other commonly used methods, and the running time is also significantly reduced.
First Offline Results from the S3 Low-Energy Branch
Authors: Romans Jekabs , Ajayakumar Anjali , Authier Martial , Boumard Frederic , Caceres Lucia , Cam Jean-François , Claessens Arno , Damoy Samuel , Delahaye Pierre , Desrues Philippe , Drouart Antoine , Duchesne Patricia , Ferrer Rafael , Fléchard Xavier , Franchoo Serge , Gangnant Patrice , de Groote Ruben P. , Kraemer Sandro , Lecesne Nathalie , Leroy Renan , Lory Julien , Lutton Franck , Manea Vladimir , Merrer Yvan , Moore Iain , Ortiz-Cortes Alejandro , Osmond Benoit , Piot Julien , Pochon Olivier , Retailleau Blaise-Maël , Savajols Hervé , Sels Simon , Traykov Emil , Uusitalo Juha , Vandamme Christophe , Vandebrouck Marine , Van den Bergh Paul , Van Duppen Piet , Verlinde Matthias , Verstraelen Elise , Wendt Klaus
Published: February 2022

We present the first results obtained from the S3 Low-Energy Branch, the gas cell setup at SPIRAL2-GANIL, which will be installed behind the S3 spectrometer for atomic and nuclear spectroscopy studies of exotic nuclei. The installation is currently being commissioned offline, with the aim to establish optimum conditions for the operation of the radio frequency quadrupole ion guides, mass separation and ion bunching, providing high-efficiency and low-energy spatial spread for the isotopes of interest. Transmission and mass-resolving power measurements are presented for the different components of the S3-LEB setup. In addition, a single-longitudinal-mode, injection-locked, pumped pulsed-titanium–sapphire laser system has been recently implemented and is used for the first proof-of-principle measurements in an offline laser laboratory. Laser spectroscopy measurements of erbium, which is the commissioning case of the S3 spectrometer, are presented using the 4f126s23H6→4f12(3H)6s6p optical transition.
An Immersible Microgripper for Pancreatic Islet and Organoid Research
Authors: Früh Eike , Bütefisch Sebastian , Gursky Benjamin , Brüning Dennis , Leester-Schädel Monika , Dietzel Andreas , Rustenbeck Ingo
Published: February 2022

To improve the predictive value of in vitro experimentation, the use of 3D cell culture models, or organoids, is becoming increasingly popular. However, the current equipment of life science laboratories has been developed to deal with cell monolayers or cell suspensions. To handle 3D cell aggregates and organoids in a well-controlled manner, without causing structural damage or disturbing the function of interest, new instrumentation is needed. In particular, the precise and stable positioning in a cell bath with flow rates sufficient to characterize the kinetic responses to physiological or pharmacological stimuli can be a demanding task. Here, we present data that demonstrate that microgrippers are well suited to this task. The current version is able to work in aqueous solutions and was shown to position isolated pancreatic islets and 3D aggregates of insulin-secreting MIN6-cells. A stable hold required a gripping force of less than 30 μN and did not affect the cellular integrity. It was maintained even with high flow rates of the bath perfusion, and it was precise enough to permit the simultaneous microfluorimetric measurements and membrane potential measurements of the single cells within the islet through the use of patch-clamp electrodes.
The Role of Systemic Microvascular Dysfunction in Heart Failure with Preserved Ejection Fraction
Authors: Weerts Jerremy , Mourmans Sanne G. J. , Barandiarán Aizpurua Arantxa , Schroen Blanche L. M. , Knackstedt Christian , Eringa Etto , Houben Alfons J. H. M. , van Empel Vanessa P. M.
Published: February 2022

Heart failure with preserved ejection fraction (HFpEF) is a condition with increasing incidence, leading to a health care problem of epidemic proportions for which no curative treatments exist. Consequently, an urge exists to better understand the pathophysiology of HFpEF. Accumulating evidence suggests a key pathophysiological role for coronary microvascular dysfunction (MVD), with an underlying mechanism of low-grade pro-inflammatory state caused by systemic comorbidities. The systemic entity of comorbidities and inflammation in HFpEF imply that patients develop HFpEF due to systemic mechanisms causing coronary MVD, or systemic MVD. The absence or presence of peripheral MVD in HFpEF would reflect HFpEF being predominantly a cardiac or a systemic disease. Here, we will review the current state of the art of cardiac and systemic microvascular dysfunction in HFpEF (Graphical Abstract), resulting in future perspectives on new diagnostic modalities and therapeutic strategies.
Biomolecule-Mediated Therapeutics of the Dentin–Pulp Complex: A Systematic Review
Authors: Machla Foteini , Angelopoulos Ioannis , Epple Matthias , Chatzinikolaidou Maria , Bakopoulou Athina
Published: February 2022

The aim of this systematic review was to evaluate the application of potential therapeutic signaling molecules on complete dentin-pulp complex and pulp tissue regeneration in orthotopic and ectopic animal studies. A search strategy was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement in the MEDLINE/PubMed database. Animal studies evaluating the application of signaling molecules to pulpectomized teeth for pulp tissue or dentin-pulp complex regeneration were included. From 2530 identified records, 18 fulfilled the eligibility criteria and were subjected to detailed qualitative analysis. Among the applied molecules, basic fibroblast growth factor, vascular endothelial growth factor, bone morphogenetic factor-7, nerve growth factor, and platelet-derived growth factor were the most frequently studied. The clinical, radiographical and histological outcome measures included healing of periapical lesions, root development, and apical closure, cellular recolonization of the pulp space, ingrowth of pulp-like connective tissue (vascularization and innervation), mineralized dentin-like tissue formation along the internal dentin walls, and odontoblast-like cells in contact with the internal dentin walls. The results indicate that signaling molecules play an important role in dentin/pulp regeneration. However, further studies are needed to determine a more specific subset combination of molecules to achieve greater efficiency towards the desired tissue engineering applications.
Utilizing Sphingomyelinase Sensitizing Liposomes in Imaging Intestinal Inflammation in Dextran Sulfate Sodium-Induced Murine Colitis
Authors: Penate Medina Tuula , Pan Jie , Damoah Christabel , Humbert Jana , Köpnick Anna-Lena , Will Olga , Sebens Susanne , Penate Medina Oula
Published: February 2022

Inflammatory bowel disease (IBD) is characterized by chronic inflammation in the gastrointestinal tract, resulting in severe symptoms. At the moment, the goal of medical treatments is to reduce inflammation. IBD is treated with systemic anti-inflammatory compounds, but they have serious side effects. The treatment that is most efficient and causes the fewest side effects would be the delivery of the drugs on the disease site. This study aimed to investigate the suitability of sphingomyelin (SM) containing liposomes to specifically target areas of inflammation in dextran sulfate sodium-induced murine colitis. Sphingomyelin is a substrate to the sphingomyelinase enzyme, which is only present outside cells in cell stress, like inflammation. When sphingomyelin consisting of liposomes is predisposed to the enzyme, it causes the weakening of the membrane structure. We demonstrated that SM-liposomes are efficiently taken up in intestinal macrophages, indicating their delivery potential. Furthermore, our studies showed that sphingomyelinase activity and release are increased in a dextran sulfate sodium-induced IBD mouse model. The enzyme appearance in IBD disease was also traced in intestine samples of the dextran sulfate sodium-treated mice and human tissue samples. The results from the IBD diseased animals, treated with fluorescently labeled SM-liposomes, demonstrated that the liposomes were taken up preferentially in the inflamed colon. This uptake efficiency correlated with sphingomyelinase activity.
Undercooling, Thermal Stability, and Application in Exothermic Catalytic Reaction of SiO2 Encapsulated SnZnCu Microspheres
Authors: Tang Haodong , Zheng Xiali , Yu Yun , Liang Jinxin , Zheng Yifan , Liu Zongjian
Published: February 2022

SiO2 encapsulated SnZnCu microspheres (several micrometers to about 30 μm in diameter) with very low undercooling, narrow freezing/melting range, and high thermal cycling stability have been produced and used as the temperature stabilizer of the packed bed in highly exothermic Fischer–Tropsch reaction. The core–shell structured SnZnCu@SiO2 microspheres are prepared in a two-step way, namely SnZnCu microspheres are firstly produced via a molten LiCl–KCl–CsCl eutectic-based metal emulsion method, and then a sol–gel approach is employed to coat them with a uniform, anti-leakage SiO2 layer. It is found that raising the amount of Zn to 4.0 at.% is critical for achieving a very low undercooling (<5 °C for SnZn0.04Cux@SiO2 vs. about 84 °C for Sn@SiO2) and a narrow freezing/melting peak width, and both undercooling and peak width are almost unchanged as the Cu content (x) increases from 1.5 to 3.0 at.%. However, their thermal cycling stability depends positively on the amount of Cu and can be remarkably improved when 3.0 at.% Cu is added. The results also show that low undercooling and narrow freezing/melting peak width are associated with the formation of Sn–Zn–Cu ternary eutectic and metastable phase Cu5Zn8, and poor thermal cycling stability of SnZn0.04Cux@SiO2 microspheres with low Cu content is related to the decomposition of Cu5Zn8 during thermal cycling. By embedding thermally stable SnZn0.04Cu0.03@SiO2 microspheres into the Co/SiO2 catalyst for Fischer–Tropsch synthesis, the temperature gradient in the catalyst bed can be significantly reduced by suppressing the formation of hot spots or thermal runaway and thus rapid deactivation of Co catalyst that occurs in the SnZn0.04Cux@SiO2-absent Co/SiO2 catalyst can be avoided.
EGCG Promotes FUS Condensate Formation in a Methylation-Dependent Manner
Authors: Lenard Aneta J. , Zhou Qishun , Madreiter-Sokolowski Corina , Bourgeois Benjamin , Habacher Hermann , Khanna Yukti , Madl Tobias
Published: February 2022

Millions of people worldwide are affected by neurodegenerative diseases (NDs), and to date, no effective treatment has been reported. The hallmark of these diseases is the formation of pathological aggregates and fibrils in neural cells. Many studies have reported that catechins, polyphenolic compounds found in a variety of plants, can directly interact with amyloidogenic proteins, prevent the formation of toxic aggregates, and in turn play neuroprotective roles. Besides harboring amyloidogenic domains, several proteins involved in NDs possess arginine-glycine/arginine-glycine-glycine (RG/RGG) regions that contribute to the formation of protein condensates. Here, we aimed to assess whether epigallocatechin gallate (EGCG) can play a role in neuroprotection via direct interaction with such RG/RGG regions. We show that EGCG directly binds to the RG/RGG region of fused in sarcoma (FUS) and that arginine methylation enhances this interaction. Unexpectedly, we found that low micromolar amounts of EGCG were sufficient to restore RNA-dependent condensate formation of methylated FUS, whereas, in the absence of EGCG, no phase separation could be observed. Our data provide new mechanistic roles of EGCG in the regulation of phase separation of RG/RGG-containing proteins, which will promote understanding of the intricate function of EGCG in cells.
Understanding Cell Lines, Patient-Derived Xenograft and Genetically Engineered Mouse Models Used to Study Cutaneous T-Cell Lymphoma
Authors: Gill Raman Preet Kaur , Gantchev Jennifer , Martínez Villarreal Amelia , Ramchatesingh Brandon , Netchiporouk Elena , Akilov Oleg E. , Ødum Niels , Gniadecki Robert , Koralov Sergei B. , Litvinov Ivan V.
Published: February 2022

Cutaneous T cell lymphoma (CTCL) is a spectrum of lymphoproliferative disorders caused by the infiltration of malignant T cells into the skin. The most common variants of CTCL include mycosis fungoides (MF), Sézary syndrome (SS) and CD30+ Lymphoproliferative disorders (CD30+ LPDs). CD30+ LPDs include primary cutaneous anaplastic large cell lymphoma (pcALCL), lymphomatoid papulosis (LyP) and borderline CD30+ LPD. The frequency of MF, SS and CD30+ LPDs is ~40–50%, <5% and ~10–25%, respectively. Despite recent advances, CTCL remains challenging to diagnose. The mechanism of CTCL carcinogenesis still remains to be fully elucidated. Hence, experiments in patient-derived cell lines and xenografts/genetically engineered mouse models (GEMMs) are critical to advance our understanding of disease pathogenesis. To enable this, understanding the intricacies and limitations of each individual model system is highly important. Presently, 11 immortalized patient-derived cell lines and different xenograft/GEMMs are being used to study the pathogenesis of CTCL and evaluate the therapeutic efficacy of various treatment modalities prior to clinical trials. Gene expression studies, and the karyotyping analyses of cell lines demonstrated that the molecular profile of SeAx, Sez4, SZ4, H9 and Hut78 is consistent with SS origin; MyLa and HH resemble the molecular profile of advanced MF, while Mac2A and PB2B represent CD30+ LPDs. Molecular analysis of the other two frequently used Human T-Cell Lymphotropic Virus-1 (HTLV-1)+ cell lines, MJ and Hut102, were found to have characteristics of Adult T-cell Leukemia/Lymphoma (ATLL). Studies in mouse models demonstrated that xenograft tumors could be grown using MyLa, HH, H9, Hut78, PB2B and SZ4 cells in NSG (NOD Scid gamma mouse) mice, while several additional experimental GEMMs were established to study the pathogenesis, effect of drugs and inflammatory cytokines in CTCL. The current review summarizes cell lines and xenograft/GEMMs used to study and understand the etiology and heterogeneity of CTCL.
Fluorescent “OFF–ON” Sensors for the Detection of Sn2+ Ions Based on Amine-Functionalized Rhodamine 6G
Authors: Rathinam Balamurugan , Murugesan Vajjiravel , Liu Bo-Tau
Published: February 2022

These structurally isomeric rhodamine 6G-based amino derivatives are designed to detect Sn2+ ions. The receptors exhibit rapid fluorescent “turn-on” responses towards Sn2+. The absorption (530 nm) and fluorescent intensity (551 nm) of the receptors increase when increasing the concentration of Sn2+. The hydrazine derivative exhibits more rapid sensitivity towards Sn2+ than the ethylene diamine derivative, indicating that the presence of an alkyl chain in the diamine decreases the sensitivity of the receptors towards Sn2+. The presence of carbonyl groups and terminal amino groups strongly influences the sensitivity of the chemosensors toward Sn2+ by a spirolactam ring-opening mechanism. The receptors exhibit 1:1 complexation with Sn2+ as evidenced by Job plot, and the corresponding limit of detection was found to be 1.62 × 10−7 M. The fluorescence images of the receptors and their complexes reveal their potential applications for imaging of Sn2+ in real/online samples.
Bioaerosols as Evidence of Atmospheric Circulation Anomalies over the Okhotsk Sea and Shantar Islands in the Late Glacial–Holocene
Authors: Razjigaeva Nadezhda , Ganzey Larisa , Grebennikova Tatiana , Ponomarev Vladimir , Mokhova Ludmila , Chakov Vladimir , Klimin Mikhail
Published: February 2022

Allochthonous biofossil distribution in the blanket peat bog of Bolshoy Shantar Island was used to analyze atmospheric circulation anomalies in the north-western Okhotsk Sea over the last 12.6 ka. The main aim of this study was to determine periods of intensification of deep cyclones and extreme storms. The composition of bioaerosols is significantly influenced by atmospheric zonal and meridional transport anomalies associated with anomalies of the monsoon system of Northeast Asia, atmospheric fronts and cyclone trajectories. Marine diatoms enter the peatland from the sea during extreme storms and record the passage of sea cyclones in the autumn-winter, whereas the distribution of allochthonous pollen indicates the intensity of continental cyclones. We used Pinus pumila pollen as an indicator of heavy snowfalls and winter cyclone activity. Fifteen phases of extreme storms were identified. Changes in ice coverage also played an important role in bioaerosol emission. During cold periods, emissions of bioaerosols mainly occurred in the open sea, whereas during warm periods, emissions occurred near the coast. The recurrence and intensity of cyclones during the cold seasons depends on displacement of the Siberian High and Aleutian Low. Periods of continental cyclones intensified in spring-summer and coincided with periods of active winter cyclogenesis.
Validation of PH and Varices Risk Scores for Prediction of High-Risk Esophageal Varix and Bleeding in Patients with B-Viral Cirrhosis
Authors: Shin Seunghwan , Kim Seung Up , Park Jun Yong , Kim Do Young , Ahn Sang Hoon , Kim Beom Kyung
Published: February 2022

Esophageal varices (EVs) can be accurately predicted using PH and varices risk scores. We aimed to validate their prognostic performances. Methods: We enrolled patients with B-viral cirrhosis as the training cohort (n = 503). Areas under receiver operating characteristic curves (AUROCs) for HEV were calculated for PH (=−5.953 + 0.188 × liver stiffness (LS) + 1.583 × sex (1:male/0:female) + 26.705 × spleen diameter/platelet count ratio) and varices (=−4.364 + 0.538 × spleen diameter −0.049 × platelet count −0.044 × LS + 0.001 × LS × platelet count) risk scores, and compared to LSPS (=LS × spleen diameter/platelet count). An independent cohort was recruited for further validation (n = 222). In the training cohort, the varices risk score showed the highest AUROC (0.926), followed by the PH risk score (0.924) and LSPS (0.924), but without any statistically significant differences. For varices risk scores ≤−1.70 and ≥1.48, a 95.0% negative predictive value (NPV) and 91.2% positive predictive value (PPV) were observed, respectively. At PH risk scores ≤2.25 and ≥7.71, 95.0% NPV and 90.0% PPV were observed, respectively. At LSPS ≤1.73 and ≥13.9, 95.3% NPV and 95.0% PPV were observed, respectively. The EV bleeding (EVB) risk during follow-up increased stepwise and significantly when stratified by PH, varices risk scores, and LSPS (all p < 0.001). In the validation cohort, NPVs were generally similar when stratified by PH (88.2%), varices risk scores (93.2%), and LSPS (88.9%); however, corresponding PPVs were suboptimal. PH and variceal risk scores are reliable for predicting HEV and future EVB. Patients with PH and varices risk scores ≤2.25 and ≤−1.70, respectively, may avoid endoscopy safely. For convenience, LSPS might be a good alternative, with comparable prognostic performance to these two models.
Stochastic Hydrodynamics of Complex Fluids: Discretisation and Entropy Production
Authors: Cates Michael E. , Fodor Étienne , Markovich Tomer , Nardini Cesare , Tjhung Elsen
Published: February 2022

Many complex fluids can be described by continuum hydrodynamic field equations, to which noise must be added in order to capture thermal fluctuations. In almost all cases, the resulting coarse-grained stochastic partial differential equations carry a short-scale cutoff, which is also reflected in numerical discretisation schemes. We draw together our recent findings concerning the construction of such schemes and the interpretation of their continuum limits, focusing, for simplicity, on models with a purely diffusive scalar field, such as ‘Model B’ which describes phase separation in binary fluid mixtures. We address the requirement that the steady-state entropy production rate (EPR) must vanish for any stochastic hydrodynamic model in a thermal equilibrium. Only if this is achieved can the given discretisation scheme be relied upon to correctly calculate the nonvanishing EPR for ‘active field theories’ in which new terms are deliberately added to the fluctuating hydrodynamic equations that break detailed balance. To compute the correct probabilities of forward and time-reversed paths (whose ratio determines the EPR), we must make a careful treatment of so-called ‘spurious drift’ and other closely related terms that depend on the discretisation scheme. We show that such subtleties can arise not only in the temporal discretisation (as is well documented for stochastic ODEs with multiplicative noise) but also from spatial discretisation, even when noise is additive, as most active field theories assume. We then review how such noise can become multiplicative via off-diagonal couplings to additional fields that thermodynamically encode the underlying chemical processes responsible for activity. In this case, the spurious drift terms need careful accounting, not just to evaluate correctly the EPR but also to numerically implement the Langevin dynamics itself.