bluuebunny/crossref_metadata_2025 huggingface dataset:
Gamma-Glutamyltransferase Is a Predictor for Future Changes of Diabetogenic Factors in Aged Chinese—A Four-Year Follow-Up Study
Authors: Wong Man Sze , Lo Chun Yen Jun , Chen Yen-Lin , Chen Fang-Yu , Kuo Chun-Heng , Chen Jin-Shuen , Pei Dee , Pitrone Pietro , Wu Chung-Ze
Published: August 2023

Glucose homeostasis in the body is determined by four diabetes factors (DFs): insulin resistance (IR), glucose effectiveness (GE), and the two phases of insulin secretion—first phase (FPIS) and second phase (SPIS). Previous research points to a correlation between elevated levels of gamma-glutamyl transferase (γGT) and an increased risk of type 2 diabetes. This study investigates the relationship between γGT and the four DFs in older Chinese individuals. This study involved 2644 men and 2598 women, all of whom were relatively healthy Chinese individuals aged 60 years or more. The DFs were calculated using formulas developed by our research, based on demographic data and factors related to metabolic syndrome. Pearson’s correlation was utilized to assess the relationship between γGT and the four DFs. The findings suggested a positive correlation between γGT and IR, FPIS, and SPIS, but a negative correlation with GE in men. Among women, only SPIS and GE were significantly correlated with γGT. The factors showed varying degrees of correlation, listed in descending order as follows: GE, SPIS, FPIS, and IR. This study confirms a significant correlation between γGT and DFs in this population, highlighting the noteworthy role of GE.
Comment on Rosa et al. Optic Nerve Drusen Evaluation: A Comparison between Ultrasound and OCT. J. Clin. Med. 2022, 11, 3715
Authors: Schimmelbusch Kurt J. , Collison Frederick T.
Published: August 2023

We read with interest the paper by Rosa et al., entitled “Optic Nerve Drusen Evaluation: A Comparison between Ultrasound and OCT”, published in June of 2022 [...]
Machine Learning Based Linking of Patient Reported Outcome Measures to WHO International Classification of Functioning, Disability, and Health Activity/Participation Categories
Authors: Habenicht Richard , Fehrmann Elisabeth , Blohm Peter , Ebenbichler Gerold , Fischer-Grote Linda , Kollmitzer Josef , Mair Patrick , Kienbacher Thomas
Published: August 2023

Background: In the primary and secondary medical health sector, patient reported outcome measures (PROMs) are widely used to assess a patient’s disease-related functional health state. However, the World Health Organization (WHO), in its recently adopted resolution on “strengthening rehabilitation in all health systems”, encourages that all health sectors, not only the rehabilitation sector, classify a patient’s functioning and health state according to the International Classification of Functioning, Disability and Health (ICF). Aim: This research sought to optimize machine learning (ML) methods that fully and automatically link information collected from PROMs in persons with unspecific chronic low back pain (cLBP) to limitations in activities and restrictions in participation that are listed in the WHO core set categories for LBP. The study also aimed to identify the minimal set of PROMs necessary for linking without compromising performance. Methods: A total of 806 patients with cLBP completed a comprehensive set of validated PROMs and were interviewed by clinical psychologists who assessed patients’ performance in activity limitations and restrictions in participation according to the ICF brief core set for low back pain (LBP). The information collected was then utilized to further develop random forest (RF) methods that classified the presence or absence of a problem within each of the activity participation ICF categories of the ICF core set for LBP. Further analyses identified those PROM items relevant to the linking process and validated the respective linking performance that utilized a minimal subset of items. Results: Compared to a recently developed ML linking method, receiver operating characteristic curve (ROC-AUC) values for the novel RF methods showed overall improved performance, with AUC values ranging from 0.73 for the ICF category d850 to 0.81 for the ICF category d540. Variable importance measurements revealed that minimal subsets of either 24 or 15 important PROM variables (out of 80 items included in full set of PROMs) would show similar linking performance. Conclusions: Findings suggest that our optimized ML based methods more accurately predict the presence or absence of limitations and restrictions listed in ICF core categories for cLBP. In addition, this accurate performance would not suffer if the list of PROM items was reduced to a minimum of 15 out of 80 items assessed.
Research on Speed Optimization and Adjusting Strategy of Variable Speed Diesel Generator Base on Sliding Interval
Authors: Wang Haiyan , Yun Qinsheng
Published: August 2023

A marine variable speed generator can dynamically adjust the set speed of a diesel engine according to the actual power load level by the grading method, so the goal of energy conservation is achieved. However, the grading method relies on engineering experience. The actual range of power load fluctuation is uncertain, which leads to a decrease in fuel efficiency. Therefore, a method based on load sliding interval is put forward. In this method, a fixed width load interval is set at first, and then the interval slides on the diesel engine load at a step of 1%. The minimum fuel consumption rate speed for the interval is optimized during the sliding process. Finally, a minimum fuel consumption rate speed curve corresponding to the sliding interval position is obtained. The optimization objective is to have the minimum weighted average fuel consumption rate at the integer load points within each interval. The actual power load is monitored and used to determine the current load interval during the calculation of the set speed. The minimum fuel consumption rate speed curve optimized was applied to query the setting value of the diesel speed. The mean value engine model was applied for simulation under three load conditions. The results show that the sliding interval method could achieve the best fuel saving effect under any power load; the set value of the diesel engine speed changed smoothly under the fluctuating power load conditions, and the fuel saving ability was better according to the actual power load conditions on the ship.
Exploring New Frontiers in Coral Nurseries: Leveraging 3D Printing Technology to Benefit Coral Growth and Survival
Authors: Berman Ofer , Levy Natalie , Parnas Haim , Levy Oren , Tarazi Ezri
Published: August 2023

Coral nurseries and associated techniques are the most common and widespread reef restoration methods worldwide. Due to the rapid decline of coral reefs, coral nurseries need to be eco-friendlier and adapted for effective upscaling to support large restoration projects. We suggest new design and fabrication processes associated with coral gardening and transplantation with 3D printing technology to offer a beneficial solution for growing coral fragments in on-land and underwater nurseries. We describe multiple combinations of building nurseries through the integration of biomimetic substrates and novel solutions for attaching coral fragments. Our methods are supported with supplemental testing of two hybrid substrate designs and coral mounting structures, building upon previous studies in the Gulf of Eilat/Aqaba (GoE/A), Red Sea. We identified and quantified marine invertebrates colonizing the surfaces of our substrates with environmental DNA (eDNA) by targeting the mitochondrial COI gene. We evaluated our coral fragments with and without our mounting structures to obtain an indication of total protein as a proxy for tissue health. We demonstrate the ability to design hybrid nurseries with custom mounting structures using biomimetic substrates, such as large ceramic artificial reefs, or with an interlocking mesh for holding numerous fragments to maximize out-planting efforts. We propose several methods for both land and underwater nurseries catered to various restoration initiatives for cost-effective up-scaling to meet the demands of global reef restoration.
High-Throughput Screening of the Repurposing Hub Library to Identify Drugs with Novel Inhibitory Activity against Candida albicans and Candida auris Biofilms
Authors: Ajetunmobi Olabayo H. , Wall Gina , Vidal Bonifacio Bruna , Martinez Delgado Lucero A. , Chaturvedi Ashok K. , Najvar Laura K. , Wormley Floyd L. , Patterson Hoja P. , Wiederhold Nathan P. , Patterson Thomas F. , Lopez-Ribot Jose L.
Published: August 2023

Candidiasis is one of the most frequent nosocomial infections affecting an increasing number of at-risk patients. Candida albicans remains the most frequent causative agent of candidiasis, but, in the last decade, C. auris has emerged as a formidable multi-drug-resistant pathogen. Both species are fully capable of forming biofilms, which contribute to resistance, increasing the urgency for new effective antifungal therapies. Repurposing existing drugs could significantly accelerate the development of novel therapies against candidiasis. Here, we have screened the Repurposing Hub library from the Broad Institute, containing over 6000 compounds, in search for inhibitors of C. albicans and C. auris biofilm formation. The primary screen identified 57 initial hits against C. albicans and 33 against C. auris. Confirmatory concentration-dependent assays were used to validate the activity of the initial hits and, at the same time, establish their anti-biofilm potency. Based on these results, ebselen, temsirolimus, and compound BAY 11-7082 emerged as the leading repositionable compounds. Subsequent experiments established their spectrum of antifungal activity against yeasts and filamentous fungi. In addition, their in vivo activity was examined in the murine models of hematogenously disseminated C. albicans and C. auris infections. Although promising, further in vitro and in vivo studies are needed to confirm their potential use for the therapy of candidiasis and possibly other fungal infections.
Four New Species of Small-Spored Alternaria Isolated from Solanum tuberosum and S. lycopersicum in China
Authors: Gou Yanan , Aung Sein Lai Lai , Guo Zhuanjun , Li Zhi , Shen Shulin , Deng Jianxin
Published: August 2023

Small-spored Alternaria species have been frequently isolated from diseased leaves of Solanum plants. To clarify the diversity of small-spored Alternaria species, a total of 118 strains were obtained from leaf samples of S. tuberosum and S. lycopersicum in six provinces of China during 2022–2023. Based on morphological characterization and multi-locus phylogenetic analysis of the internal transcribed spacer of the rDNA region (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), translation elongation factor 1 alpha (TEF1), RNA polymerase second largest subunit (RPB2), Alternaria major allergen gene (Alt a 1), endopolygalacturonase gene (EndoPG) and an anonymous gene region (OPA10-2), seven species were determined, including four novel species and three known species (A. alternata, A. gossypina and A. arborescens). The novel species were described and illustrated as A. longxiensis sp. nov., A. lijiangensis sp. nov., A. lycopersici sp. nov. and A. solanicola sp. nov.. In addition, the pathogenicity of the seven species was evaluated on potato leaves. The species exhibited various aggressiveness, which could help in disease management.
Longitudinal Analysis of the ASES and Constant–Murley Scores, and the Internal Rotation/Shift and Jobe Tests Following Arthroscopic Repair of Supraspinatus Lesions
Authors: Fieseler George , Laudner Kevin , Cornelius Jakob , Schulze Stephan , Delank Karl-Stefan , Schwesig René
Published: August 2023

It is essential to investigate patients post-surgery using functional surveys like the American Shoulder and Elbow Surgeons Shoulder (ASES) and the Constant–Murley shoulder (CMS) scores, as well as clinical tests, such as the Internal Rotation and Shift (IRO/Shift) and Jobe tests. In this study, 51 out of an initial 87 patients underwent an arthroscopic supraspinatus repair (22 single-row, 16 double-row, 13 debridement). Testing occurred pre-surgery, and 3 and 6 months post-surgery. Both surveys showed significant improvements over time among all 87 patients, but there were no differences between groups (lesion/no lesion) (p > 0.815) or time × group (p > 0.895). The IRO/Shift test showed a stronger ability to distinguish between both groups (positive vs. negative) with respect to the ASES and CMS scores over time, but the Jobe test did not (p > 0.100). Improvements in the CMS scores and the Jobe test were lower following repair compared to the ASES and IRO/Shift test. Most patients returned to adequate levels of functional abilities at 6 months post-surgery. The time required to return to activities of daily living and negative clinical tests was longer for the double-row repair patients compared to the single-row and debridement groups. In conclusion, both the functional surveys and the clinical tests demonstrated improvements following surgery.
Prognostic Value of Serum Biomarkers in Patients with Idiopathic Pulmonary Fibrosis in Relation to Disease Progression
Authors: Domvri Kalliopi , Organtzis Ioannis , Apostolopoulos Apostolos , Fouka Evangelia , Kontakiotis Theodoros , Papakosta Despoina
Published: August 2023

Background: The aim of this present study was to determine serum biomarker levels and their correlation with respiratory function and the clinical course of patients with idiopathic pulmonary fibrosis (IPF). Materials and Methods: This study included 72 IPF patients, according to the ATS/ERS criteria, in whom antifibrotic treatment was initiated. Blood samples were taken, and serum biomarkers, such as KL-6, SP-D, CCL18, CXCL13, VEGF-A, IL-8, IGFBP-1, IGFBP-2, IGFBP-7 and ICAM-1 were measured using ELISA methodology. Pulmonary function tests (FVC, TLC, DLCO-% pred) were determined at baseline and after 12 and 24 months and analyzed in correlation with the biomarkers. Results: The majority of patients (mean age 72 ± 6 years) were men (83%). The FVC and DLCO values at the 12-month follow-up were found to be statistically decreased in deceased patients (p < 0.05). The SP-D (p < 0.001) and the IGFBP-1 (p = 0.021) levels were found to be increased at the 1-year follow-up in deceased patients, and similarly, the SP-D (p = 0.005) and ICAM-1 (p = 0.043) levels at the 2-year follow-up. A chi-square test revealed that 70% of the category IV GAP index was found with cut-off elevated levels of a biomarker combination (KL-6, SP-D, VEGF-A) from the ROC curve analysis (p < 0.05). Conclusion: This study provides evidence, for the first time in a Greek population, of the possibility of using a combination of KL-6, SP-D, and VEGF-A serum levels along with the GAP index.
No Association of Angiotensin-Converting Enzyme Insertion/Deletion (ACE I/D) Gene Polymorphism in the Susceptibility to Diabetic Retinopathy in Type 2 Diabetes Mellitus Patients: An Updated Meta-Analysis
Authors: Coelho Aline Ruilowa de Pinho , Silveira Luciana Carvalho , Santos Kamilla de Faria , Santos Rodrigo da Silva , Reis Angela Adamski da Silva
Published: August 2023

Diabetic retinopathy (DR) is a complex and multifactorial pathology encompassing environmental, metabolic, and polygenic influences. Among the genes possibly involved in the development and progression of DR, the Angiotensin I-converting enzyme (ACE) gene stands out, which presents an insertion (I) or deletion (D) polymorphism of a 287 bp Alu repetitive sequence in intron 16. Thus, this study aimed to perform a systematic review with meta-analysis to elucidate the relationship between the ACE gene (I/D) polymorphism (rs1799752) and the development and progression of DR in type 2 diabetic patients. PubMed/MEDLINE, Embase, Web of Science, and Scopus databases were systematically searched to retrieve articles that investigated the association between ACE gene (I/D) polymorphism in DR patients. Sixteen articles were included in the systematic review. The results describe no significant association between the polymorphism and DR risk (OR = 1.12; CI = 0.96–1.31; and p = 0.1359) for genotypic analysis by the dominant model (II vs. ID+DD). Moreover, we also observed no significant association between the D allele on the allele frequency analysis (I vs. D) and the DR risk (OR = 1.10; CI = 0.98–1.23; and p = 0.1182). Forest plot analysis revealed that the discrepancy between previous studies most likely arose from variations in their sample sizes. In conclusion, I/D polymorphism appears to be not involved in the susceptibility to and progression of the DR in type 2 diabetic patients.
Health-Related Quality of Life and Stress-Related Disorders in Patients with Bronchiectasis after Pulmonary Resection
Authors: Nicola Alin , Oancea Cristian , Barata Paula Irina , Adelina Mavrea , Mateescu Tudor , Manolescu Diana , Bratosin Felix , Fericean Roxana Manuela , Pingilati Raja Akshay , Paleru Cristian
Published: August 2023

This multicenter, cross-sectional study investigates the potential correlation between the development of bronchiectasis after lung resection surgery and the health-related quality of life (HRQoL) of the patients. The study aims to provide new insights into the long-term outcomes of patients post-lung resection surgery. The study includes adult patients who underwent lung resection surgery for suspicious lung nodules and developed bronchiectasis within a follow-up period of six months. Bronchiectasis was confirmed by high-resolution computed tomography scans. The patient’s health-related quality of life (HRQoL), anxiety, depression, and stress-related disorders were assessed using WHOQOL-BREF, SF-36, HADS, and PSS-10 questionnaires. Out of the 135 patients included in the study, 44 developed bronchiectasis after lung resection surgery. No statistically significant differences were observed between the groups in terms of demographics and medical history. Patients with bronchiectasis demonstrated a lower overall health status, increased deterioration of respiratory symptoms, lower physical activity levels, lower quality of life scores, and experienced more severe anxiety symptoms. Additionally, patients in this group also perceived higher levels of stress; although, the correlation with physical functioning was contradictory. The development of bronchiectasis post-lung resection surgery was associated with poorer quality of life, increased respiratory symptoms, higher anxiety levels, and increased perception of stress. While the correlation between bronchiectasis and HRQoL was statistically significant, the contradictory correlations with stress and physical functioning call for further research. This study underscores the importance of ongoing patient monitoring and the detailed evaluation of respiratory function following lung resection surgery for lung nodules, especially among those who develop bronchiectasis.
Foot Temperature by Infrared Thermography in Patients with Peripheral Artery Disease before and after Structured Home-Based Exercise: A Gender-Based Observational Study
Authors: Crepaldi Anna , Caruso Lorenzo , Piva Giovanni , Traina Luca , Gasbarro Vincenzo , Manfredini Roberto , Lamberti Nicola , Rinaldo Natascia , Manfredini Fabio , Lopez-Soto Pablo Jesus
Published: August 2023

Decreased arterial perfusion is a typical condition of patients with peripheral artery disease (PAD), with the microvascular picture particularly present among women. This observational study aimed to detect foot perfusion changes by infrared thermography (IRT) after a home-based exercise program in both sexes. A total of 76 PAD patients with claudication (72 ± 4 years; 52 males) were enrolled in a structured in-home exercise program composed of two daily 8 min interval walking sessions (1:1 walk:rest ratio) with progressively increasing speed. Outcome measures collected at baseline (T0) and at each hospital visit after 5 weeks, 12 weeks and 20 weeks included foot temperature measured by IRT (anterior tibial, posterior tibial, dorsalis pedis and arcuate artery regions), ankle brachial index and the 6 min walking test. After 20 weeks, foot temperature in both limbs showed a significant increasing trend, with a mean variation of 1.3 °C for the more impaired limb and 0.9 °C for the contralateral limb (t = 8.88, p < 0.001 and t = 5.36; p < 0.001, respectively), with significant changes occurring after 5 weeks of training. The sex-oriented analysis did not highlight any significant difference, with an improvement of mean foot temperature of 1.5 ± 0.6 °C in females versus 1.2 ± 0.5 °C in males (p = 0.42). Ankle brachial index and performance also significantly improved over time (p < 0.001) without gender differences. In patients with PAD, a structured low-intensity exercise program significantly improved foot temperature and exercise capacity without any sex-related difference.
Class I HDAC Inhibition Leads to a Downregulation of FANCD2 and RAD51, and the Eradication of Glioblastoma Cells
Authors: Drzewiecka Małgorzata , Jaśniak Dominika , Barszczewska-Pietraszek Gabriela , Czarny Piotr , Kobrzycka Anna , Wieczorek Marek , Radek Maciej , Szemraj Janusz , Skorski Tomasz , Śliwiński Tomasz
Published: August 2023

HDAC inhibitors (HDACi) hold great potential as anticancer therapies due to their ability to regulate the acetylation of both histone and non-histone proteins, which is frequently disrupted in cancer and contributes to the development and advancement of the disease. Additionally, HDACi have been shown to enhance the cytotoxic effects of DNA-damaging agents such as radiation and cisplatin. In this study, we found that histone deacetylase inhibits valproic acid (VPA), synergized with PARP1 inhibitor (PARPi), talazoparib (BMN-673), and alkylating agent, and temozolomide (TMZ) to induce DNA damage and reduce glioblastoma multiforme. At the molecular level, VPA leads to a downregulation of FANCD2 and RAD51, and the eradication of glioblastoma cells. The results of this study indicate that combining HDACi with PARPi could potentially enhance the treatment of glioblastoma, the most aggressive type of cancer that originates in the brain.
Effect of Metformin on the Functional and Electrophysiological Recovery of Crush Injury-Induced Facial Nerve Paralysis in Diabetic Rats
Authors: Sun Kyung Hoon , Choi Cheol Hee , Cho Gwang-Won , Jang Chul Ho
Published: August 2023

The impact of metformin on the rat facial nerve following crush injury has only occasionally been documented to date. The purpose of the current investigation was to use functional and electrophysiological evaluations to investigate the effects of metformin administration on recovery following crush injury to the rat facial nerve. The rats were randomly divided into four groups: the nonDM/PBS group (n = 4), the nonDM/metformin group (n = 4), the DM/PBS group (n = 4), and the DM/metformin group (n = 4). Diabetes was generated by an intraperitoneal injection of streptozotocin. Facial nerve paralysis was induced by a crush injury 7 days after diabetes induction. The blood glucose levels of the DM/PBS and DM/metformin groups were maintained at over 300 mg/dL, whereas the blood glucose levels of the nonDM/PBS and nonDM/metformin groups were maintained at less than 150 mg/dL. There was no significant difference between the two nonDM groups. In comparison to the PBS group, the metformin group’s recurrence of vibrissa fibrillation occurred noticeably sooner over time. The nonDM/metformin group showed the highest recovery rate in the second, third, and fourth weeks post-crush, respectively. The threshold of action potential 4 weeks after crush injury showed that the nonDM/metformin group had a significantly lower mean threshold of MAP compared to other groups. The short-term effect of metformin on the recovery of facial nerve blood flow (FNBF) was significantly increased compared to the DM/PBS group. However, there was no significant difference in FNBF between the nonDM/metformin and nonDM/PBS groups. A diabetic condition promoted a delay in FN regeneration. Metformin is able to accelerate functional recovery in diabetic or nondiabetic FN-injured rats. Further studies using a morphometric or molecular approach are planned to understand the pharmacologic mechanism of metformin.
Obesity and Hyperandrogenemia in Polycystic Ovary Syndrome: Clinical Implications
Authors: López-Alarcón Mardia , Vital-Reyes Víctor Saúl , Almeida-Gutiérrez Eduardo , Maldonado-Hernández Jorge , Flores-Chávez Salvador , Domínguez-Salgado Juan Manuel , Vite-Bautista José , Cruz-Martínez David , Barradas-Vázquez Aly S. , Z’Cruz-López Ricardo
Published: August 2023

Polycystic ovary syndrome (PCOS) is often accompanied with metabolic disturbances attributed to androgen excess and obesity, but the contribution of each has not been defined, and the occurrence of metabolic disturbances is usually not investigated. Ninety-nine women with PCOS and forty-one without PCOS were evaluated. The clinical biomarkers of alterations related to glucose (glucose, insulin, and clamp-derived glucose disposal − M), liver (aspartate aminotransferase, alanine aminotransferase, and gamma-glutamyl transferase), and endothelium (arginine, asymmetric dymethylarginine, carotid intima-media thickness, and flow-mediated dilation) metabolism were measured; participants were categorized into four groups according to their obesity (OB) and hyperandrogenemia (HA) status as follows: Healthy (no-HA, lean), HA (HA, lean), OB (no-HA, OB), and HAOB (HA, OB). Metabolic disturbances were very frequent in women with PCOS (≈70%). BMI correlated with all biomarkers, whereas free testosterone (FT) correlated with only glucose- and liver-related indicators. Although insulin sensitivity and liver enzymes were associated with FT, women with obesity showed lower M (coef = 8.56 − 0.080(FT) − 3.71(Ob); p < 0.001) and higher aspartate aminotransferase (coef = 26.27 + 0.532 (FT) + 8.08 (Ob); p = 0.015) than lean women with the same level of FT. Women with obesity showed a higher risk of metabolic disorders than lean women, independent of hyperandrogenemia. Clinicians are compelled to look for metabolic alterations in women with PCOS. Obesity should be treated in all cases, but hyperandrogenemia should also be monitored in those with glucose-or liver-related disturbances.
Potential of the Digital Information Platform Applied in Promotion of Rural Landscape in China: From Preliminary Practices to Framework Conception
Authors: Shu Yang , Ma Xiaoqian , Liu Zhiying , Li Jun , Zhang Yawei
Published: August 2023

The rural landscape is an integrated system of tangible and intangible cultural heritages in rural areas, which includes multiple attributes in terms of culture, spirit and nature. The multi-dimensional, holistic protection and promotion of the rural landscape is considered to be a challenging topic in the academic circle. In the past two decades, digital information platforms have been widely used in the field of protecting cultural heritage because of their convenience, as well as their cross-temporal and systematic nature. However, a digital information platform that is specifically designed for a rural landscape is still in the initial stage of experimentation and exploration, and there is a lack of systematic understanding and overall framework conception. In this paper, with the preliminary practice of the rural landscape digital information platform (RLDIP) in China in the past two decades as the example, a combination of qualitative and quantitative analysis is conducted to deeply explore the data source, data type, data organization and functional structure of the platform samples. The common characteristics, potential in application and typical problems of the current platform are summarized. What is more, based on the need for a comprehensive rural revitalization in such developing countries as China, a framework conception of the rural landscape digital information platform (RLDIP) is proposed, and its data-classification system, functional structure and digital process are identified. This study will provide a theoretical basis and technical reference for the digital information platform to develop its potential in overall protection, coordinated development and sustainable management of the rural landscape in the future.
Spatiotemporal Linkages between Administrative Division Adjustment and Urban Form: Political Drivers of the Urban Polycentric Structure
Authors: Chen Youlin , Wang Lei , Yu Peiheng , Nie Ning , Yang Xuan , Chen Yiyun
Published: August 2023

As a crucial government strategy for spatial management and resource allocation, administrative division adjustment (ADA) provides interesting insights in the investigation of the polycentric urban structure (POLY). Using high-resolution geographic grid population data, this study aims to interpret complex linkages between ADA and POLY. Specifically, this research explores the dynamic evolution of POLY and ADA, investigates the spatiotemporal impact of ADA on POLY based on geographically and temporally weighted regression models and analyzes the differences in socioeconomic driving forces for POLY in cities with and without ADA. The results demonstrate that the value of POLY had a rising trend during the study periods. In terms of spatial pattern, POLY has a higher value in the Eastern region and a lower value in the Western region. The influences of ADA on POLY are also characterized by spatiotemporal heterogeneity. The impact of ADA on POLY has a higher value in Eastern and Western China and a lower value in Central and Northeastern China. In addition, the impacts of socioeconomic factors on POLY between cities with and without ADA differed significantly in Central and Western China while differing insignificantly in Eastern and Northeastern China. To promote the balanced development of administrative institutional structures and urban spatial transformation, ADA should be selectively implemented to facilitate POLY following the level of population, economic and productive development in each region.
Spatial Equality of Urban Care Facilities from the Perspective of Refinement: An Example from Changchun, China
Authors: Zhang Jiayi , Chen Yingzi , Hu Yaqi , Lai Lina
Published: August 2023

Care facilities are the mainstay of social services for the elderly. As the trend of empty nesting among elderly families intensifies due to the aging population, it is becoming difficult for families to take on the responsibility of caring for the elderly on their own, and the elderly need more formal care to be provided by society. The contradiction between the lag in the construction of care facilities and the growth in the social demand for elderly care has been highlighted, and the spatial equality of care facilities is an important means of achieving an optimal allocation of resources for elderly care. In this paper, we use the two-step floating catchment method to measure the spatial accessibility of care facilities from a fine-grained perspective, using big data, including mobile phone signaling data and points of interest. Then, we use the Gini coefficient to analyze the equality of the spatial allocations of different types of urban care facilities. The results of the study show that, first, the spatial equality of care facilities is good in terms of the Gini coefficient, but the overall spatial accessibility of care facilities is at a relatively low level compared to that in developed cities. This means that the layout of Changchun’s care facilities is good, but there is insufficient service capacity. Second, the spatial accessibility of residential care facilities is characterized by a gradual decline, with high levels in the core and low levels in the periphery. Fewer facilities are located in the peripheral areas of the city, and spatial accessibility is at a lower level, a characteristic different from that seen in cities with a higher level of economic development, such as Beijing. Third, the spatial accessibility of community care facilities showed a polycentric, contiguous distribution. The effect of the study scale on the spatial accessibility of community care facilities was more pronounced, with large scales obscuring the high values of the spatial accessibility of community care facilities in peripheral areas. Fourth, Changchun has better overall equality of care facilities, with differences in the different types and areas of care provided. The spatial balance of care facilities in the core area is better than that observed in other regions. In conclusion, this paper underscores the value of big data, such as mobile phone signaling data, in supporting refined urban research. Furthermore, our comprehensive analysis of social care facility equality can inform policy making and spatial planning.
The Impact of Urbanization on Food Security: A Case Study of Jiangsu Province
Authors: Kang Jiayu , Duan Xuejun , Yun Ruxian
Published: August 2023

Food security has received extensive academic attention in recent years. However, research results analyzing cultivated land pressure from the perspective of urbanization are relatively few. This study used Jiangsu Province as the study area and analyzed the spatial pattern evolution of cultivated land pressure from 2005 to 2019 by constructing a formula for a cultivated land pressure index. The study used a spatial econometric approach to analyze the spatial relationship between urbanization and cultivated land pressure. Based on the spatial Durbin model, the impact of urbanization on the pressure on cultivated land is analyzed. According to the results, Jiangsu Province showed an obvious north–south divergence in the spatial distribution of the cultivated land pressure index, with the low-value areas of the cultivated land pressure index mainly distributed in northern and central Jiangsu, and the high-value areas mainly distributed in southern Jiangsu. The urbanization level and cultivated land pressure level in Jiangsu Province showed obvious spatial clustering characteristics, and there was a certain overlap between the high- and low-value clustering areas of the two, with significant positive spatial correlation features. The total urbanization had no significant effect on the cultivated land pressure. Population urbanization and industry urbanization showed a significant negative effect on cultivated land pressure in Jiangsu Province, while land urbanization showed a positive effect. Both population and land urbanization had a significant negative spatial spillover effect on cultivated land pressure. Plausible explanations of these results were provided and policy implications were drawn.
Redox Modification of PKA-Cα Differentially Affects Its Substrate Selection
Authors: Delva-Wiley Jeannette , Ekhator Ese S. , Adams Laquaundra L. , Patwardhan Supriya , Dong Ming , Newman Robert H.
Published: August 2023

The cyclic AMP-dependent protein kinase (PKA) plays an essential role in the regulation of many important cellular processes and is dysregulated in several pervasive diseases, including diabetes, cardiovascular disease, and various neurodegenerative disorders. Previous studies suggest that the alpha isoform of the catalytic subunit of PKA (PKA-Cα) is oxidized on C199, both in vitro and in situ. However, the molecular consequences of these modifications on PKA-Cα’s substrate selection remain largely unexplored. C199 is located on the P + 1 loop within PKA-Cα’s active site, suggesting that redox modification may affect its kinase activity. Given the proximity of C199 to the substrate binding pocket, we hypothesized that oxidation could differentially alter PKA-Cα’s activity toward its substrates. To this end, we examined the effects of diamide- and H2O2-dependent oxidation on PKA-Cα’s activity toward select peptide and protein substrates using a combination of biochemical (i.e., trans-phosphorylation assays and steady-state kinetics analysis) and biophysical (i.e., surface plasmon resonance and fluorescence polarization assays) strategies. These studies suggest that redox modification of PKA-Cα differentially affects its activity toward different substrates. For instance, we found that diamide-mediated oxidation caused a marked decrease in PKA-Cα’s activity toward some substrates (e.g., Kemptide and CREBtide) while having little effect on others (e.g., Crosstide). In contrast, H2O2-dependent oxidation of PKA-Cα led to an increase in its activity toward each of the substrates at relatively low H2O2 concentrations, with differential effects at higher peroxide concentrations. Together, these studies offer novel insights into crosstalk between redox- and phosphorylation-dependent signaling pathways mediated by PKA. Likewise, since C199 is highly conserved among AGC kinase family members, they also lay the foundation for future studies designed to elucidate the role of redox-dependent modification of kinase substrate selection in physiological and pathological states.
Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation
Authors: Garella Rachele , Bernacchioni Caterina , Chellini Flaminia , Tani Alessia , Palmieri Francesco , Parigi Martina , Guasti Daniele , Cassioli Emanuele , Castellini Giovanni , Ricca Valdo , Bani Daniele , Sassoli Chiara , Donati Chiara , Squecco Roberta
Published: August 2023

Adipokines are peptide hormones produced by the adipose tissue involved in several biological functions. Among adipokines, adiponectin (ADPN) has antidiabetic and anti-inflammatory properties. It can also modulate food intake at central and peripheral levels, acting on hypothalamus and facilitating gastric relaxation. ADPN exerts its action interacting with two distinct membrane receptors and triggering some well-defined signaling cascades. The ceramidase activity of ADPN receptor has been reported in many tissues: it converts ceramide into sphingosine. In turn, sphingosine kinase (SK) phosphorylates it into sphingosine-1 phosphate (S1P), a crucial mediator of many cellular processes including contractility. Using a multidisciplinary approach that combined biochemical, electrophysiological and morphological investigations, we explored for the first time the possible role of S1P metabolism in mediating ADPN effects on the murine gastric fundus muscle layer. By using a specific pharmacological inhibitor of SK2, we showed that ADPN affects smooth muscle cell membrane properties and contractile machinery via SK2 activation in gastric fundus, adding a piece of knowledge to the action mechanisms of this hormone. These findings help to identify ADPN and its receptors as new therapeutic targets or as possible prognostic markers for diseases with altered energy balance and for pathologies with fat mass content alterations.
Bilateral Angle Recession and Chronic Post-Traumatic Glaucoma: A Review of the Literature and a Case Report
Authors: Iannucci Valeria , Manni Priscilla , Alisi Ludovico , Mecarelli Giulia , Lambiase Alessandro , Bruscolini Alice
Published: August 2023

Ocular trauma affects millions of people worldwide and is a leading cause of secondary glaucoma. Angle recession is the main cause of post-traumatic glaucoma after blunt eye trauma, and it is usually unilateral. The aim of this paper is to investigate the possible causes of angle recession with a bilateral presentation. Airbag activation during traffic accidents is a likely cause to be ruled out, along with repeated head or eye trauma, due to contact sports or a history of physical abuse. These aspects can aid in early detection, appropriate management, and improved outcomes for patients with ocular trauma. Finally, we report the case of a 75-year-old Caucasian man who developed a bilateral angle recession after an airbag impact, with advanced glaucoma in the right eye and ocular hypertension in the left eye. To our knowledge, this is the first case in the literature of chronic post-traumatic glaucoma probably caused by an airbag.
Membrane Lipid Composition Influences the Hydration of Proton Half-Channels in FoF1-ATP Synthase
Authors: Ivontsin Leonid A. , Mashkovtseva Elena V. , Nartsissov Yaroslav R.
Published: August 2023

The membrane lipid composition plays an important role in the regulation of membrane protein activity. To probe its influence on proton half-channels’ structure in FoF1-ATP synthase, we performed molecular dynamics simulations with the bacterial protein complex (PDB ID: 6VWK) embedded in three types of membranes: a model POPC, a lipid bilayer containing 25% (in vivo), and 75% (bacterial stress) of cardiolipin (CL). The structure proved to be stable regardless of the lipid composition. The presence of CL increased the hydration of half-channels. The merging of two water cavities at the inlet half-channel entrance and a long continuous chain of water molecules directly to cAsp61 from the periplasm were observed. Minor conformational changes in half-channels with the addition of CL caused extremely rare direct transitions between aGlu219-aAsp119, aGlu219-aHis245, and aGln252-cAsp61. Deeper penetration of water molecules (W1–W3) also increased the proton transport continuity. Stable spatial positions of significant amino acid (AA) residue aAsn214 were found under all simulation conditions indicate a prevailing influence of AA-AA or AA-W interactions on the side-chain dynamics. These results allowed us to put forward a model of the proton movement in ATP synthases under conditions close to in vivo and to evaluate the importance of membrane composition in simulations.
Effect of Feedstock Powder Intrinsic Characteristics on the Tribological Behavior of Inductively Remelted NiCrBSi Flame-Sprayed Coatings
Authors: Muntean Roxana , Vălean Petru-Cristian , Kazamer Norbert , Uțu Ion-Dragoș , Mărginean Gabriela , Șerban Viorel Aurel
Published: August 2023

Ni-based alloys are among the materials of choice in developing high-quality coatings for ambient and high-temperature applications that require protection against intense wear and corrosion. The current study aims to develop and characterize NiCrBSi coatings with high wear resistance and improved adhesion to the substrate. Starting with nickel-based feedstock powders, thermally sprayed coatings were initially fabricated. Prior to deposition, the powders were characterized in terms of microstructure, particle size, chemical composition, flowability, and density. For comparison, three types of powders with different chemical compositions and characteristics were deposited onto a 1.7227 tempered steel substrate using oxyacetylene flame spraying, and subsequently, the coatings were inductively remelted. Ball-on-disc sliding wear testing was chosen to investigate the tribological properties of both the as-sprayed and induction-remelted coatings. The results reveal that, in the case of as-sprayed coatings, the main wear mechanisms were abrasive, independent of powder chemical composition, and correlated with intense wear losses due to the poor intersplat cohesion typical of flame-sprayed coatings. The remelting treatment improved the performance of the coatings in terms of wear compared to that of the as-sprayed ones, and the density and lower porosity achieved during the induction post-treatment had a significant positive role in this behavior.
Lightweight Image Denoising Network for Multimedia Teaching System
Authors: Zhang Xuanyu , Tian Chunwei , Zhang Qi , Gan Hong-Seng , Cheng Tongtong , Ibrahim Mohd Asrul Hery
Published: August 2023

Due to COVID-19, online education has become an important tool for teachers to teach students. Also, teachers depend on a multimedia teaching system (platform) to finish online education. However, interacted images from a multimedia teaching system may suffer from noise. To address this issue, we propose a lightweight image denoising network (LIDNet) for multimedia teaching systems. A parallel network can be used to mine complementary information. To achieve an adaptive CNN, an omni-dimensional dynamic convolution fused into an upper network can automatically adjust parameters to achieve a robust CNN, according to different input noisy images. That also enlarges the difference in network architecture, which can improve the denoising effect. To refine obtained structural information, a serial network is set behind a parallel network. To extract more salient information, an adaptively parametric rectifier linear unit composed of an attention mechanism and a ReLU is used into LIDNet. Experiments show that our proposed method is effective in image denoising, which can also provide assistance for multimedia teaching systems.
Event-Triggered State Estimation for Uncertain Systems with Binary Encoding Transmission Scheme
Authors: Li Zun , Xue Binqiang , Chen Youyuan
Published: August 2023

This paper proposes an event-triggered state estimation method for parameter-uncertain systems with a binary encoding transmission scheme. Firstly, a binary encoding transmission scheme is introduced between the state estimator and the system to improve the efficiency of network communication. Secondly, an event-triggering mechanism (ETM) is designed to ensure the accuracy of state estimation and reduce the computational burden of the state estimator. At the event-triggered moments, considering the uncertainty of the system, the binary encoding transmission scheme, and the ETM, a moving horizon estimator (MHER) is designed using the robust least squares optimization method to obtain optimal state estimation. At the no-event-triggered moments, the state estimation of the system is computed based on an open-loop state estimator (OLER). Furthermore, stability analysis showed that the state estimation error of the proposed method is bounded. Finally, the practical value of the proposed in this paper is confirmed through numerical simulation.
Parallel Dense Video Caption Generation with Multi-Modal Features
Authors: Huang Xuefei , Chan Ka-Hou , Ke Wei , Sheng Hao
Published: August 2023

The task of dense video captioning is to generate detailed natural-language descriptions for an original video, which requires deep analysis and mining of semantic captions to identify events in the video. Existing methods typically follow a localisation-then-captioning sequence within given frame sequences, resulting in caption generation that is highly dependent on which objects have been detected. This work proposes a parallel-based dense video captioning method that can simultaneously address the mutual constraint between event proposals and captions. Additionally, a deformable Transformer framework is introduced to reduce or free manual threshold of hyperparameters in such methods. An information transfer station is also added as a representation organisation, which receives the hidden features extracted from a frame and implicitly generates multiple event proposals. The proposed method also adopts LSTM (Long short-term memory) with deformable attention as the main layer for caption generation. Experimental results show that the proposed method outperforms other methods in this area to a certain degree on the ActivityNet Caption dataset, providing competitive results.