bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of the Discrepancy Principle on the Tikhonov-Regularized Solutions with Oversmoothing Penalties
Authors: Hofmann Bernd , Hofmann Christopher
Published: March 2020

This paper deals with the Tikhonov regularization for nonlinear ill-posed operator equations in Hilbert scales with oversmoothing penalties. One focus is on the application of the discrepancy principle for choosing the regularization parameter and its consequences. Numerical case studies are performed in order to complement analytical results concerning the oversmoothing situation. For example, case studies are presented for exact solutions of Hölder type smoothness with a low Hölder exponent. Moreover, the regularization parameter choice using the discrepancy principle, for which rate results are proven in the oversmoothing case in in reference (Hofmann, B.; Mathé, P. Inverse Probl. 2018, 34, 015007) is compared to Hölder type a priori choices. On the other hand, well-known analytical results on the existence and convergence of regularized solutions are summarized and partially augmented. In particular, a sketch for a novel proof to derive Hölder convergence rates in the case of oversmoothing penalties is given, extending ideas from in reference (Hofmann, B.; Plato, R. ETNA. 2020, 93).
Improved Compact Cuckoo Search Algorithm Applied to Location of Drone Logistics Hub
Authors: Pan Jeng-Shyang , Song Pei-Cheng , Chu Shu-Chuan , Peng Yan-Jun
Published: March 2020

Drone logistics can play an important role in logistics at the end of the supply chain and special environmental logistics. At present, drone logistics is in the initial development stage, and the location of drone logistics hubs is an important issue in the optimization of logistics systems. This paper implements a compact cuckoo search algorithm with mixed uniform sampling technology, and, for the problem of weak search ability of the algorithm, this paper combines the method of recording the key positions of the search process and increasing the number of generated solutions to achieve further improvements, as well as implements the improved compact cuckoo search algorithm. Then, this paper uses 28 test functions to verify the algorithm. Aiming at the problem of the location of drone logistics hubs in remote areas or rural areas, this paper establishes a simple model that considers the traffic around the village, the size of the village, and other factors. It is suitable for selecting the location of the logistics hub in advance, reducing the cost of drone logistics, and accelerating the large-scale application of drone logistics. This paper uses the proposed algorithm for testing, and the test results indicate that the proposed algorithm has strong competitiveness in the proposed model.
An Upfront Two-Stent Strategy for True Coronary Bifurcation Lesions with A Large Side Branch in Acute Coronary Syndrome: A Two-Year Follow-Up Study
Authors: Yurtdaş Mustafa , Asoğlu Ramazan , Özdemir Mahmut , Asoğlu Emin
Published: March 2020

Background and Objectives: Little is known about the upfront two-stent strategy (U2SS) for true coronary bifurcation lesions (CBLs) in acute coronary syndrome (ACS). We aimed to present our two-year follow-up results on the U2SS by using different two-stent techniques for the true CBL with a large side branch (SB) in ACS patients, including unstable angina (UA), non-ST-segment elevation myocardial infarction (NSTEMI), and ST-segment elevation myocardial infarction (STEMI), and to identify independent predictors of the presence of major adverse cardiac events (MACEs) after intervention. Materials and Methods: The study included 201 consecutive ACS patients with true CBLs who underwent percutaneous coronary intervention (PCI) using U2SS from October 2015 to March 2018. Clinical outcomes at follow-up were assessed. MACE was defined as a composite of cardiac death, non-fatal myocardial infarction, and target lesion revascularization (TLR). Results: 31.3% of the patients had an UA, 46.3% had an NSTEMI, and 22.4% had an STEMI. CBL was most frequently located in the left anterior descending (LAD)/diagonal artery (59.2%). In total, 71.1% of the patients had a Medina classification (1,1,1). Overall, 62.2% of cases were treated with mini-crush stenting. Clopidogrel was given in 23.9% of the patients; 71.1% of the patients received everolimus eluting stent (EES); and 11.9% received a sirolimus eluting stent (SES). Final kissing balloon inflation was carried out in all patients, with an unsatisfactory rate of 5%. A proximal optimization technique sequence was successfully carried out in all patients. The MACE incidence was 16.9% with a median follow-up period of 2.1 years. There were seven cardiac deaths (3.5%). The TLR rate was 13.4% (n = 27), with PCI treatment in 16 patients, and coronary artery bypass grafting treatment in 11 patients. After multivariate penalized logistic regression analysis (Firth logistic regression), clopidogrel use (odds ratio (OR): 2.19; 95% confidence interval (CI): 0.41–2.51; p = 0.007) and SES use (OR: 1.86; 95% CI: 0.31–2.64; p = 0.014) were independent predictors of the presence of MACE. Conclusion: U2SS is feasible and safe for the true CBLs with large and diseased SB in ACS patients, and is related to a relatively low incidence of MACE. Clopidogrel use and SES use may predict the MACE development in ACS patients treated using U2SS.
Estimation of Caries Treatment Needs in First Permanent Molars of Lithuanian 5–6-Year-Old Children, Based on Caries Lesion Activity Assessment
Authors: Mačiulskienė Vita , Razmienė Jaunė , Andruškevičienė Vilija , Bendoraitienė Eglė
Published: March 2020

Background and Objectives: Early detection of dental caries lesions at active stages of development can facilitate their monitoring and reduce needs for restorative dental care. This study aimed to describe the prevalence and caries treatment needs in first permanent molars of pre-school children, based on a caries lesion activity assessment, and in relation to participants’ ages, dental plaque levels and toothbrushing habits. Materials and Methods: Large cross-sectional dental caries survey using multistage cluster sampling was conducted among Lithuanian 4–6-year-old children attending kindergartens. For the present study purpose, all individuals presenting erupted permanent molars were selected. Thus, only 5–6-year-olds (n = 453) took part in this study. They were examined for caries by one calibrated examiner using Nyvad clinical diagnostic criteria that differentiate between active and inactive caries lesions. Dental plaque was assessed by the Silness-Löe index, and parents’ reports about toothbrushing frequency were collected. Results: Overall, 41% of permanent molars were affected by caries; 6-year-olds had more caries lesions than 5-year-olds (p < 0.05). Mean number of decayed and filled surfaces (DF-S) of all participants was 1.79 (SD 2.93), half of lesions were noncavitated, more than one-third were cavitated and fillings comprised less than one surface per child. Majority of lesions were active; prevalence of inactive lesions (all noncavitated) was 1% and 6% in 5- and 6-year-olds, respectively. Prevalence of active lesions increased with age; it correlated with plaque levels and with toothbrushing frequency (<0.001). Likelihood to detect active lesions was up to nine times higher in teeth with abundant plaque (odds ratio (OR) 8.73; confidence interval (CI) 5.35–14.25), and up to seven times higher in individuals brushing teeth irregularly (OR 6.88; CI 2.21–21.41). Conclusions: The obtained data indicate high treatment needs in the erupted permanent molars of the Lithuanian pre-school population and imply that caries management should primarily focus on improved biofilm removal, accompanied with regular use of fluoridated toothpaste.
Impact of Changes in Serum Calcium Levels on In-Hospital Mortality
Authors: Thongprayoon Charat , Cheungpasitporn Wisit , Hansrivijit Panupong , Medaura Juan , Chewcharat Api , Mao Michael A , Bathini Tarun , Vallabhajosyula Saraschandra , Thirunavukkarasu Sorkko , Erickson Stephen B.
Published: March 2020

Background and objectives: Calcium concentration is strictly regulated at both the cellular and systemic level, and changes in serum calcium levels can alter various physiological functions in various organs. This study aimed to assess the association between changes in calcium levels during hospitalization and mortality. Materials and Methods: We searched our patient database to identify all adult patients admitted to our hospital from January 1st, 2009 to December 31st, 2013. Patients with ≥2 serum calcium measurements during the hospitalization were included. The serum calcium changes during the hospitalization, defined as the absolute difference between the maximum and the minimum calcium levels, were categorized into five groups: 0–0.4, 0.5–0.9, 1.0–1.4, 1.5–1.9, and ≥2.0 mg/dL. Multivariable logistic regression was performed to assess the independent association between calcium changes and in-hospital mortality, using the change in calcium category of 0–0.4 mg/dL as the reference group. Results: Of 9868 patients included in analysis, 540 (5.4%) died during hospitalization. The in-hospital mortality progressively increased with higher calcium changes, from 3.4% in the group of 0–0.4 mg/dL to 14.5% in the group of ≥2.0 mg/dL (p < 0.001). When adjusted for age, sex, race, principal diagnosis, comorbidity, kidney function, acute kidney injury, number of measurements of serum calcium, and hospital length of stay, the serum calcium changes of 1.0–1.4, 1.5–1.9, and ≥2.0 mg/dL were significantly associated with increased in-hospital mortality with odds ratio (OR) of 1.55 (95% confidence interval (CI) 1.15–2.10), 1.90 (95% CI 1.32–2.74), and 3.23 (95% CI 2.39–4.38), respectively. The association remained statistically significant when further adjusted for either the lowest or highest serum calcium. Conclusion: Larger serum calcium changes in hospitalized patients were progressively associated with increased in-hospital mortality.
The Risk of Para-Aortic Lymph Node Metastases in Apparent Early Stage Ovarian Cancer
Authors: Bacalbasa Nicolae , Balescu Irina , Vilcu Mihaela , Dima Simona , Diaconu Camelia , Iliescu Laura , Filipescu Alexandru , Dimitriu Mihai , Brezean Iulian
Published: March 2020

Background and objectives: To identify the risk factors for para-aortic lymph node metastases in cases with presumed early stage ovarian cancer. Materials and methods: Between 2014 and 2019, 48 patients with apparent early stage ovarian cancer were submitted to surgery. In all cases, pelvic and para-aortic lymph node dissection was performed for staging purposes. Results: Among the 48 cases we identified nine cases with positive pelvic lymph nodes and 11 cases with positive para-aortic lymph nodes. The positivity of the retrieved lymph nodes was significantly correlated with the histopathological subtype represented by serous histology (p = 0.02), as well as with the degree of differentiation (p = 0.004). Conclusions: Patients with serous ovarian carcinomas in association with a poorer degree of differentiation are at risk of associated lymph node metastases even in presumed early stages of the disease. Therefore, lymph node dissection should be performed in such cases in order to provide adequate staging and tailoring of further treatment.
Recycling Chain for Spent Lithium-Ion Batteries
Authors: Werner Denis , Peuker Urs Alexander , Mütze Thomas
Published: March 2020

The recycling of spent lithium-ion batteries (LIB) is becoming increasingly important with regard to environmental, economic, geostrategic, and health aspects due to the increasing amount of LIB produced, introduced into the market, and being spent in the following years. The recycling itself becomes a challenge to face on one hand the special aspects of LIB-technology and on the other hand to reply to the idea of circular economy. In this paper, we analyze the different recycling concepts for spent LIBs and categorize them according to state-of-the-art schemes of waste treatment technology. Therefore, we structure the different processes into process stages and unit processes. Several recycling technologies are treating spent lithium-ion batteries worldwide focusing on one or several process stages or unit processes.
Quasi-Static and Dynamic Testing of Metallic Materials
Authors: Westermann Ida
Published: March 2020

Understanding the deformation of metallic materials at different states is essential in forming operations and under working conditions [...]
Metabolite Shifts Induced by Marathon Race Competition Differ between Athletes Based on Level of Fitness and Performance: A Substudy of the Enzy-MagIC Study
Authors: Schader Jana F. , Haid Mark , Cecil Alexander , Schoenfeld Julia , Halle Martin , Pfeufer Arne , Prehn Cornelia , Adamski Jerzy , Nieman David C. , Scherr Johannes
Published: March 2020

This study compared metabolite shifts induced by training for, participation in, and recovery from a marathon race competition among athletes divided into three groups based on fitness (relative maximum oxygen uptake (VO2max)) and performance levels (net running time). Plasma samples from 76 male runners participating in the Munich Marathon were analyzed for metabolite shifts using a targeted metabolomics panel. For the entire cohort of runners, pronounced increases were measured immediately after the race for plasma concentrations of acylcarnitines (AC), the ratio (palmitoylcarnitine + stearoylcarnitine)/free carnitine that is used as a proxy for the activity of the mitochondrial enzyme carnitine palmitoyltransferase, and arginine-related metabolites, with decreases in most amino acids (AA) and phospholipids. Plasma levels of AA and phospholipids were strongly increased 24 and 72 h post-race. Post-race plasma concentrations of AC and arginine-related metabolites were higher in the low compared to top performers, indicating an accumulation of fatty acids and a reliance on protein catabolism to provide energy after the marathon event. This study showed that marathon race competition is associated with an extensive and prolonged perturbation in plasma metabolite concentrations with a strong AC signature that is greater in the slower, less aerobically fit runners. Furthermore, changes in the arginine-related metabolites were observed.
Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity
Authors: Araujo-Garrido Juan Luis , Bernal-Bayard Joaquín , Ramos-Morales Francisco
Published: March 2020

Type III secretion systems are used by many Gram-negative bacterial pathogens to inject proteins, known as effectors, into the cytosol of host cells. These virulence factors interfere with a diverse array of host signal transduction pathways and cellular processes. Many effectors have catalytic activities to promote post-translational modifications of host proteins. This review focuses on a family of effectors with glycosyltransferase activity that catalyze addition of N-acetyl-d-glucosamine to specific arginine residues in target proteins, leading to reduced NF-κB pathway activation and impaired host cell death. This family includes NleB from Citrobacter rodentium, NleB1 and NleB2 from enteropathogenic and enterohemorrhagic Escherichia coli, and SseK1, SseK2, and SseK3 from Salmonella enterica. First, we place these effectors in the general framework of the glycosyltransferase superfamily and in the particular context of the role of glycosylation in bacterial pathogenesis. Then, we provide detailed information about currently known members of this family, their role in virulence, and their targets.
Eukaryotic Cell Permeabilisation to Identify New Putative Chlamydial Type III Secretion System Effectors Secreted within Host Cell Cytoplasm
Authors: Kebbi-Beghdadi Carole , Pilloux Ludovic , Martin Virginie , Greub Gilbert
Published: March 2020

Chlamydia trachomatis and Waddlia chondrophila are strict intracellular bacteria belonging to the Chlamydiales order. C. trachomatis is the most frequent bacterial cause of genital and ocular infections whereas W. chondrophila is an opportunistic pathogen associated with adverse pregnancy outcomes and respiratory infections. Being strictly intracellular, these bacteria are engaged in a complex interplay with their hosts to modulate their environment and create optimal conditions for completing their life cycle. For this purpose, they possess several secretion pathways and, in particular, a Type III Secretion System (T3SS) devoted to the delivery of effector proteins in the host cell cytosol. Identifying these effectors is a crucial step in understanding the molecular basis of bacterial pathogenesis. Following incubation of infected cells with perfringolysin O, a pore-forming toxin that binds cholesterol present in plasma membranes, we analysed by mass spectrometry the protein content of the host cell cytoplasm. We identified 13 putative effectors secreted by C. trachomatis and 19 secreted by W. chondrophila. Using Y. enterocolitica as a heterologous expression and secretion system, we confirmed that four of these identified proteins are secreted by the T3SS. Two W. chondrophila T3SS effectors (hypothetical proteins Wcw_0499 and Wcw_1706) were further characterised and demonstrated to be early/mid-cycle effectors. In addition, Wcw_1706 is associated with a tetratricopeptide domain-containing protein homologous to C. trachomatis class II chaperone. Furthermore, we identified a novel C. trachomatis effector, CT460 that localises in the eukaryotic nucleus when ectopically expressed in 293 T cells.
Yeast Associated with Rice Phylloplane and Their Contribution to Control of Rice Sheath Blight Disease
Authors: Into Parichat , Khunnamwong Pannida , Jindamoragot Sasitorn , Am-in Somjit , Intanoo Wanwilai , Limtong Savitree
Published: March 2020

The phylloplane is an important habitat for yeasts and these yeasts may have antagonistic activities against pathogens and could be used as biocontrol agents. To investigate rice phylloplane yeasts, 282 strains were isolated from 89 rice leaf samples and identified as 15 known yeast species in the phylum Ascomycota and 35 known and two potential new species in the phylum Basidiomycota. The majority of rice phylloplane yeasts belonged to the phylum Basidiomycota. The evaluation of antagonistic activities of 83 yeast strains against rice pathogenic fungi Pyricularia oryzae, Rhizoctonia solani, Fusarium moniliforme, Helminthosporium oryzae and Curvularia lunata revealed that 14 strains inhibited these pathogens. Among the antagonistic strains, Torulaspora indica DMKU-RP31, T. indica DMKU-RP35 and Wickerhamomyces anomalus DMKU-RP25 inhibited all rice pathogens tested, and the production of volatile organic compounds, fungal cell wall degrading enzymes and biofilm were the possible antagonistic mechanisms against all rice pathogens tested in vitro. These yeast strains were evaluated for controlling rice sheath blight caused by R. solani in rice plants in the greenhouse and were found to suppress the disease by 60.0–70.3%, whereas 3% validamycin suppressed by 83.8%. Therefore, they have potential for being developed to be used as biocontrol agents for rice sheath blight.
Insights on Structure and Threshold Detection Limits of Stichtite (Magnesium-Chromium Carbonate-Hydroxide) by Fourier Transform Infrared Analysis
Authors: Melchiorre Erik , Garcia Andy , Brounce Maryjo
Published: March 2020

Spectral features for natural stichtite at 1042, 1096, 1360, 1638, and 3482 cm−1 provide insights on mineral structure, with peaks consistent with OH− stretching modes bound to Mg or Cr, CO32− antisymmetric stretches and CO32− bound within the sample and molecular water. These Infrared (IR) data suggest natural stichtite forms at a pH of >12 with increased water and decreased carbonate in the interlayer due to a smaller interlayer distance and unit cell. Higher pH favors lower divalent cation purity and may explain observed ranges of non-end member compositions in stichtite from localities around the world, and across geologic time. This constrains stichtite formation to a range of very high pH conditions and is consistent with active serpentinizing fluid vents and some mine wastes. IR has clear application for the detection and quantification of stichtite under field and laboratory conditions within the detection limits of 5% stichtite within a serpentine host. The size and grade of terrestrial stichtite deposits, and resolution of remote sensing instruments, suggest remote IR detection of stichtite is possible, and remote IR detection for Earth and Mars is discussed.
Effect of Hydrodynamic Cavitation Assistance on Different Stages of Coal Flotation
Authors: Han Hua , Liu An , Wang Huaifa
Published: March 2020

In this study, the effect of hydrodynamic cavitation (HC) on the conditioning stage (HCCS), separation stage (HCSS), and whole stage (HCWS) of coal flotation was investigated by flotation tests, laser granulometry, and contact angle measurements. The flotation results indicate that compared to conventional flotation, all HC-assisted flotation methods can improve concentrate combustible recovery and flotation constant rate. HCCS and HCSS show similar levels of improvement, while HCWS has a better flotation efficiency. The screening tests demonstrate that HC has the advantage of being able to liberate coarse coal particles (+0.25 mm) prior to being combined with gangues. On one hand, HC promotes the dispersion of both particles and agents, while longer cavitation time in HCCS does not lead to better flotation performance. On the other hand, enhancement of the adsorption of the collector on the surface of coal particles in HCCS is confirmed by flotation concentrate contact angle tests. However, HCSS leads to a decrease in concentrate hydrophobicity, compared to conventional flotation. The micro-nanobubbles generated by HC play an important role in improving flotation performance. HCWS offers the advantages of both HCCS and HCSS, and the cooperated mechanism of different HC modes enhances the recovery of coal particles in flotation.
Novel Strategy for Improvement of the Bioleaching Efficiency of Acidithiobacillus ferrooxidans Based on the AfeI/R Quorum Sensing System
Authors: Gao Xue-Yan , Liu Xiu-Jie , Fu Chang-Ai , Gu Xiu-Feng , Lin Jian-Qiang , Liu Xiang-Mei , Pang Xin , Lin Jian-Qun , Chen Lin-Xu
Published: March 2020

Acidithiobacillus ferrooxidans is an acidophilic and chemolithotrophic sulfur- and iron-oxidizing bacterium that has been widely used in the bioleaching process for extracting metals. Extracellular polymeric substances (EPS) are essential for bacteria-ore interactions, and the regulation of EPS synthesis could be an important way of influencing the efficiency of the bioleaching process. Therefore, exploring and utilizing the regulatory pathways of EPS synthesis to improve the bacterial bioleaching capability have posed a challenge in the study and application of bioleaching bacteria. Here, several engineering strains were constructed using genetic manipulation methods. And we revealed the regulatory function of the AfeI/R quorum sensing (QS) system in EPS synthesis and biofilm formation of A. ferrooxidans, and the AfeI/R-mediated EPS synthesis could influence bacteria-substrate interactions and the efficiency of bioleaching. Finally, an AfeI/R-mediated bioleaching model was proposed to illustrate the role of QS system in this process. This study provided new insights into and clues for developing highly efficient bioleaching bacteria and modulating the bioleaching process.
3-Aryl-4-nitrobenzothiochromans S,S-dioxide: From Calcium-Channel Modulators Properties to Multidrug-Resistance Reverting Activity
Authors: Micucci Matteo , Viale Maurizio , Chiarini Alberto , Spinelli Domenico , Frosini Maria , Tavani Cinzia , Maccagno Massimo , Bianchi Lara , Gangemi Rosaria , Budriesi Roberta
Published: March 2020

Our research groups have been involved for many years in studies aimed at identifying new active organic compounds endowed with pharmacological properties. In this work, we focused our attention on the evaluation of cardiovascular and molecular drug resistance (MDR) reverting activities of some nitrosubstituted sulphur-containing heterocycles. Firstly, we have examined the effects of 4-nitro-3-(4-methylphenyl)-3,6-dihydro-2H-thiopyran S,S-dioxide 5, and have observed no activity. Then we have extended our investigation to the 3-aryl-4-nitrobenzothiochromans S,S-dioxide 6 and 7, and have observed an interesting biological profile. Cardiovascular activities were assessed for all compounds using ex vivo studies, while the MDR reverting effect was evaluated only for selected compounds using tumor cell lines. All compounds were shown to affect cardiovascular parameters. Compound 7i exerted the most effect on negative inotropic activity, while 6d and 6f could be interesting molecules for the development of more active ABCB1 inhibitors. Both 6 and 7 represent structures of large possible biological interest, providing a scaffold for the identification of new ABCB1 inhibitors.
Impact of the Cathode Pt Loading on PEMFC Contamination by Several Airborne Contaminants
Authors: St-Pierre Jean , Zhai Yunfeng
Published: March 2020

Proton exchange membrane fuel cells (PEMFCs) with 0.1 and 0.4 mg Pt cm−2 cathode catalyst loadings were separately contaminated with seven organic species: Acetonitrile, acetylene, bromomethane, iso-propanol, methyl methacrylate, naphthalene, and propene. The lower catalyst loading led to larger cell voltage losses at the steady state. Three closely related electrical equivalent circuits were used to fit impedance spectra obtained before, during, and after contamination, which revealed that the cell voltage loss was due to higher kinetic and mass transfer resistances. A significant correlation was not found between the steady-state cell voltage loss and the sum of the kinetic and mass transfer resistance changes. Major increases in research program costs and efforts would be required to find a predictive correlation, which suggests a focus on contamination prevention and recovery measures rather than contamination mechanisms.
Stability and Extraction of Vanillin and Coumarin under Subcritical Water Conditions
Authors: Doctor Ninad , Parker Grayson , Vang Katie , Smith Melanie , Kayan Berkant , Yang Yu
Published: March 2020

In order to facilitate the development of the green subcritical water chromatography technique for vanillin and coumarin, the stability of the compounds under subcritical water conditions was investigated in this work. In addition, their extraction from natural products was also studied. The stability experiments were carried out by heating the mixtures of vanillin and water or coumarin and water at temperatures ranging from 100 °C to 250 °C, while subcritical water extractions (SBWE) of both analytes from vanilla beans and whole tonka beans were conducted at 100 °C to 200 °C. Analyte quantification for both stability and extraction studies was carried out by HPLC. After heating for 60 min, vanillin was found to be stable in water at temperatures up to 250 °C. While coumarin is also stable at lower temperatures such as 100 °C and 150 °C, it undergoes partial degradation after heating for 60 min at 200 °C and higher. The results of this stability study support green subcritical water chromatographic separation and extraction of vanillin and coumarin at temperatures up to 150 °C. The SBWE results revealed that the extraction efficiency of both analytes from vanilla beans and tonka beans is significantly improved with increasing temperature.
Is There a Future for PPARs in the Treatment of Neuropsychiatric Disorders?
Authors: Tufano Michele , Pinna Graziano
Published: March 2020

Recently, peroxisome proliferator-activated receptor (PPAR)-α and γ isoforms have been gaining consistent interest in neuropathology and treatment of neuropsychiatric disorders. Several studies have provided evidence that either the receptor expression or the levels of their endogenously-produced modulators are downregulated in several neurological and psychiatric disorders and in their respective animal models. Remarkably, administration of these endogenous or synthetic ligands improves mood and cognition, suggesting that PPARs may offer a significant pharmacological target to improve several neuropathologies. Furthermore, various neurological and psychiatric disorders reflect sustained levels of systemic inflammation. Hence, the strategy of targeting PPARs for their anti-inflammatory role to improve these disorders is attracting attention. Traditionally, classical antidepressants fail to be effective, specifically in patients with inflammation. Non-steroidal anti-inflammatory drugs exert potent antidepressant effects by acting along with PPARs, thereby strongly substantiating the involvement of these receptors in the mechanisms that lead to development of several neuropathologies. We reviewed running findings in support of a role for PPARs in the treatment of neurological diseases, including Alzheimer’s disease or psychiatric disorders, such as major depression. We discuss the opportunity of targeting PPARs as a future pharmacological approach to decrease neuropsychiatric symptoms at the same time that PPAR ligands resolve neuroinflammatory processes.
Protonated and Cationic Helium Clusters
Authors: Lundberg Linnea , Bartl Peter , Leidlmair Christian , Scheier Paul , Gatchell Michael
Published: March 2020

Protonated rare gas clusters have previously been shown to display markably different structures compared to their pure, cationic counterparts. Here, we have performed high-resolution mass spectrometry measurements of protonated and pristine clusters of He containing up to 50 atoms. We identify notable differences between the magic numbers present in the two types of clusters, but in contrast to heavier rare gas clusters, neither the protonated nor pure clusters exhibit signs of icosahedral symmetries. These findings are discussed in light of results from heavier rare gases and previous theoretical work on protonated helium.
Peptides from Natural or Rationally Designed Sources Can Be Used in Overweight, Obesity, and Type 2 Diabetes Therapies
Authors: Gewehr Mayara C. F. , Silverio Renata , Rosa-Neto José Cesar , Lira Fabio S. , Reckziegel Patrícia , Ferro Emer S.
Published: March 2020

Overweight and obesity are among the most prominent health problems in the modern world, mostly because they are either associated with or increase the risk of other diseases such as type 2 diabetes, hypertension, and/or cancer. Most professional organizations define overweight and obesity according to individual body–mass index (BMI, weight in kilograms divided by height squared in meters). Overweight is defined as individuals with BMI from 25 to 29, and obesity as individuals with BMI ≥30. Obesity is the result of genetic, behavioral, environmental, physiological, social, and cultural factors that result in energy imbalance and promote excessive fat deposition. Despite all the knowledge concerning the pathophysiology of obesity, which is considered a disease, none of the existing treatments alone or in combination can normalize blood glucose concentration and prevent debilitating complications from obesity. This review discusses some new perspectives for overweight and obesity treatments, including the use of the new orally active cannabinoid peptide Pep19, the advantage of which is the absence of undesired central nervous system effects usually experienced with other cannabinoids.
Laboratory-Scale Preparation and Characterization of Dried Extract of Muirapuama (Ptychopetalum olacoides Benth) by Green Analytical Techniques
Authors: Trindade Ester Paulitsch , Regis Franklin Teixeira , Araújo da Silva Gabriel , Nunes Aguillar Breno , de Paiva Amorim Marcelo Vítor , Ramos Ferreira George Leandro , Soares Aragão Cícero Flávio , da Silva Solon Lílian Grace
Published: March 2020

This work reports on the preparation of a drying process from the ethanolic extract of Muirapuama and its characterization through green analytical techniques. The spray-drying processes were performed by using ethanolic extract in a ratio of 1:1 extract/excipient and 32 factorial design. The properties of dried powder were investigated in terms of total flavonoid content, moisture content, powder yield, and particle size distribution. An analytical eco-scale was applied to assess the greenness of the developed protocol. Ultra-high performance liquid chromatography (UHPLC)with reduced solvent consumption in the analysis was compared to the conventional HPLC method. A Fourier transform near-infrared (FT-NIR) spectroscopic method was applied based on the principal component scores for the prediction of extract/excipient mixtures and partial least squares regression model for quantitative analysis. NIR spectroscopy is an economic, powerful, and fast methodology for the detection of excipient in muirapuama dried extracts, generating no residue in the analysis. Scanning electron microscopy (SEM) images showed samples with a higher concentration of excipient, presenting better morphological characteristics and a lower moisture absorption rate. An eco-scale score value of 85 was achieved for UHPLC and 100 was achieved for NIR (excellent green analysis). Above all, these methods are rapid and green for the routine analysis of herbal medicines based on dried extracts.
A Cataluminescence Sensor Based on NiO Nanoparticles for Sensitive Detection of Acetaldehyde
Authors: Zhang Run-Kun , Wang Die , Wu Yan-Jun , Hu Yi-Han , Chen Jian-Yu , He Jin-Can , Wang Jing-Xin
Published: March 2020

Sensitive and selective detection of harmful gas is an important task in environmental monitoring. In this work, a gas sensor based on cataluminescence (CTL) for detection of acetaldehyde was designed by using nano-NiO as the sensing material. The sensor shows sensitive response to acetaldehyde at a relatively low working temperature of 200 °C. The linear range of CTL intensity versus acetaldehyde concentration is 0.02–2.5 mg/L, with a limit of detection of 0.006 mg/L at a signal-to-noise ratio of three. Mechanism study shows that electronically excited CO2 is the excited intermediate for CTL emission during the catalytic oxidation of acetaldehyde on the NiO surface. The proposed sensor has promising application in monitoring acetaldehyde in residential buildings and in the workplace.
Electro-Optical Properties of Monolayer and Bilayer Pentagonal BN: First Principles Study
Authors: Amiri Mehran , Beheshtian Javad , Shayeganfar Farzaneh , Faghihnasiri Mahdi , Shahsavari Rouzbeh , Ramazani Ali
Published: March 2020

Two-dimensional hexagonal boron nitride (hBN) is an insulator with polar covalent B-N bonds. Monolayer and bilayer pentagonal BN emerge as an optoelectronic material, which can be used in photo-based devices such as photodetectors and photocatalysis. Herein, we implement spin polarized electron density calculations to extract electronic/optical properties of mono- and bilayer pentagonal BN structures, labeled as B 2 N 4 , B 3 N 3 , and B 4 N 2 . Unlike the insulating hBN, the pentagonal BN exhibits metallic or semiconducting behavior, depending on the detailed pentagonal structures. The origin of the metallicity is attributed to the delocalized boron (B) 2p electrons, which has been verified by electron localized function and electronic band structure as well as density of states. Interestingly, all 3D networks of different bilayer pentagonal BN are dynamically stable unlike 2D structures, whose monolayer B 4 N 2 is unstable. These 3D materials retain their metallic and semiconductor nature. Our findings of the optical properties indicate that pentagonal BN has a visible absorption peak that is suitable for photovoltaic application. Metallic behavior of pentagonal BN has a particular potential for thin-film based devices and nanomaterial engineering.
Improving Formation Conditions and Properties of hBN Nanosheets Through BaF2-assisted Polymer Derived Ceramics (PDCs) Technique
Authors: Matsoso Boitumelo , Vuillet-a-Ciles Victor , Bois Laurence , Toury Bérangère , Journet Catherine
Published: March 2020

Hexagonal boron nitrite (hBN) is an attractive material for many applications such as in electronics as a complement to graphene, in anti-oxidation coatings, light emitters, etc. However, the synthesis of high-quality hBN at cost-effective conditions is still a great challenge. Thus, this work reports on the synthesis of large-area and crystalline hBN nanosheets via the modified polymer derived ceramics (PDCs) process. The addition of both the BaF2 and Li3N, as melting-point reduction and crystallization agents, respectively, led to the production of hBN powders with excellent physicochemical properties at relatively low temperatures and atmospheric pressure conditions. For instance, XRD, Raman, and XPS data revealed improved crystallinity and quality at a decreased formation temperature of 1200 °C upon the addition of 5 wt% of BaF2. Moreover, morphological determination illustrated the formation of multi-layered nanocrystalline and well-defined shaped hBN powders with crystal sizes of 2.74–8.41 ± 0.71 µm in diameter. Despite the compromised thermal stability, as shown by the ease of oxidation at high temperatures, this work paves way for the production of large-scale and high-quality hBN crystals at a relatively low temperature and atmospheric pressure conditions.
Immune Phenotype and Functionality of Mtb-Specific T-Cells in HIV/TB Co-Infected Patients on Antiretroviral Treatment
Authors: Mupfumi Lucy , Mpande Cheleka A.M. , Reid Tim , Moyo Sikhulile , Shin Sanghyuk S. , Zetola Nicola , Mogashoa Tuelo , Musonda Rosemary M. , Kasvosve Ishmael , Scriba Thomas J. , Nemes Elisa , Gaseitsiwe Simani
Published: March 2020

The performance of host blood-based biomarkers for tuberculosis (TB) in HIV-infected patients on antiretroviral therapy (ART) has not been fully assessed. We evaluated the immune phenotype and functionality of antigen-specific T-cell responses in HIV positive (+) participants with TB (n = 12) compared to HIV negative (−) participants with either TB (n = 9) or latent TB infection (LTBI) (n = 9). We show that the cytokine profile of Mtb-specific CD4+ T-cells in participants with TB, regardless of HIV status, was predominantly single IFN-γ or dual IFN-γ/ TNFα. Whilst ESAT-6/CFP-10 responding T-cells were predominantly of an effector memory (CD27−CD45RA−CCR7−) profile, HIV-specific T-cells were mainly of a central (CD27+CD45RA−CCR7+) and transitional memory (CD27+CD45RA+/−CCR7−) phenotype on both CD4+ and CD8+ T-cells. Using receiving operating characteristic (ROC) curve analysis, co-expression of CD38 and HLA-DR on ESAT-6/CFP-10 responding total cytokine-producing CD4+ T-cells had a high sensitivity for discriminating HIV+TB (100%, 95% CI 70–100) and HIV−TB (100%, 95% CI 70–100) from latent TB with high specificity (100%, 95% CI 68–100 for HIV−TB) at a cut-off value of 5% and 13%, respectively. TB treatment reduced the proportion of Mtb-specific total cytokine+CD38+HLA-DR+ CD4+ T-cells only in HIV−TB (p = 0.001). Our results suggest that co-expression of CD38 and HLA-DR on Mtb-specific CD4+ T-cells could serve as a TB diagnosis tool regardless of HIV status.
The B Subunit of PirABvp Toxin Secreted from Vibrio parahaemolyticus Causing AHPND Is an Amino Sugar Specific Lectin
Authors: Victorio-De Los Santos Marcelo , Vibanco-Pérez Norberto , Soto-Rodriguez Sonia , Pereyra Ali , Zenteno Edgar , Cano-Sánchez Patricia
Published: March 2020

Vibrio parahaemolyticus (Vp) is the etiological agent of the acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimp. Vp possesses a 63–70 kb conjugative plasmid that encodes the binary toxin PirAvp/PirBvp. The 250 kDa PirABvp complex was purified by affinity chromatography with galactose-sepharose 4B and on a stroma from glutaraldehyde-fixed rat erythrocytes column, as a heterotetramer of PirAvp and PirBvp subunits. In addition, recombinant pirB (rPirBvp) and pirA (rPirAvp) were obtained. The homogeneity of the purified protein was determined by SDS-PAGE analysis, and the yield of protein was 488 ng/100 μg of total protein of extracellular products. The PirABvp complex and the rPirBvp showed hemagglutinating activity toward rat erythrocytes. The rPirAvp showed no hemagglutinating capacity toward the animal red cells tested. Among different mono and disaccharides tested, only GalNH2 and GlcNH2 were able to inhibit hemagglutination of the PirABvp complex and the rPirBvp. Glycoproteins showed inhibitory specificity, and fetuin was the glycoprotein that showed the highest inhibition. Other glycoproteins, such as mucin, and glycosaminoglycans, such as heparin, also inhibited the activity. Desialylation of erythrocytes enhanced the hemagglutinating activity. This confirms that Gal or Gal (β1,4) GlcNAc are the main ligands for PirABvp. The agglutinating activity of the PirABvp complex and the rPirBvp is not dependent on cations, because addition of Mg2+ or Ca2+ showed no effect on the protein capacity. Our results strongly suggest that the PirBvp subunit is a lectin, which is part of the PirA/PirBvp complex, and it seems to participate in bacterial pathogenicity.
Synthesis and Properties of Phosphoric-Acid-Doped Polybenzimidazole with Hyperbranched Cross-Linkers Decorated with Imidazolium Groups as High-Temperature Proton Exchange Membranes
Authors: Gao Chunmei , Hu Meishao , Wang Li , Wang Lei
Published: March 2020

Highly phosphoric-acid (PA)-doped polybenzimidazole (PBI) membranes exhibit good proton conductivity at high temperatures; however, they suffer from reduced mechanical properties and loss of PA molecules due to the plasticity of PA and the weak interactions between PA and benzimidazoles, especially with the absorption of water. In this work, a series of PBIs with hyperbranched cross-linkers decorated with imidazolium groups (ImOPBI-x, where x is the weight ratio of the hyperbranched cross-linker) as high-temperature proton exchange membranes are designed and synthesized for the first time. We observe how the hyperbranched cross-linkers can endow the membranes with improved oxidative stability and acceptable mechanical performance, and imidazolium groups with strong basicity can stabilize the PA molecules by delocalization and hydrogen bond formation to endow the membranes with an enhanced proton conductivity and a decreased loss of PA molecules. We measured a high proton conductivity of the ImOPBI-x membranes, ranging from 0.058 to 0.089 S cm−1 at 160 °C. In addition, all the ImOPBI-x membranes displayed good mechanical and oxidative properties. At 160 °C, a fuel cell based on the ImOPBI-5 membrane showed a power density of 638 mW cm−2 and good durability under a hydrogen/oxygen atmosphere, indicating its promising use in anhydrous proton exchange membrane applications.
Coarsening Kinetics of Complex Macromolecular Architectures in Bad Solvent
Authors: Paciolla Mariarita , Arismendi-Arrieta Daniel J. , Moreno Angel J.
Published: March 2020

This study reports a general scenario for the out-of-equilibrium features of collapsing polymeric architectures. We use molecular dynamics simulations to characterize the coarsening kinetics, in bad solvent, for several macromolecular systems with an increasing degree of structural complexity. In particular, we focus on: flexible and semiflexible polymer chains, star polymers with 3 and 12 arms, and microgels with both ordered and disordered networks. Starting from a powerful analogy with critical phenomena, we construct a density field representation that removes fast fluctuations and provides a consistent characterization of the domain growth. Our results indicate that the coarsening kinetics presents a scaling behaviour that is independent of the solvent quality parameter, in analogy to the time–temperature superposition principle. Interestingly, the domain growth in time follows a power-law behaviour that is approximately independent of the architecture for all the flexible systems; while it is steeper for the semiflexible chains. Nevertheless, the fractal nature of the dense regions emerging during the collapse exhibits the same scaling behaviour for all the macromolecules. This suggests that the faster growing length scale in the semiflexible chains originates just from a faster mass diffusion along the chain contour, induced by the local stiffness. The decay of the dynamic correlations displays scaling behavior with the growing length scale of the system, which is a characteristic signature in coarsening phenomena.
Novel Approaches for Intensifying Negative C60 Ion Beams Using Conventional Ion Sources Installed on a Tandem Accelerator
Authors: Chiba Atsuya , Usui Aya , Hirano Yoshimi , Yamada Keisuke , Narumi Kazumasa , Saitoh Yuichi
Published: March 2020

We developed novel methods for producing negative C60 ion beams at the accelerator facility Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) to increase the current intensity of swift C60 ion beams accelerated to the MeV energy region using a tandem accelerator. We produced negative C60 ion beams with an intensity of 1.3 µA, which is several tens of thousands of times greater than the intensity of beams produced using conventional methods based on the Cs sputtering process. These beams were obtained by temporarily adding an ionization function based on electron attachment to an existing ion source that is widely used in tandem accelerators. The high-intensity swift C60 ion beams can be made available relatively easily to institutes that have tandem accelerators and ion sources of the type used at TIARA because there is no need to change existing ion sources or install new ones.
Collective Action in Waste Management: A Comparative Study of Recycling and Recovery Initiatives from Brazil, Indonesia, and Nigeria Using the Institutional Analysis and Development Framework
Authors: Oh Jinkyung , Hettiarachchi Hiroshan
Published: March 2020

Recycling and recovery provide not only a sustainable option to decrease the volume of waste that needs final disposal, but also a blueprint to a circular economy. However, rates of recycling/recovery still remain very low on a global scale. While it is important to look for technology-based solutions to improve recycling/recovery activities, such solutions might not be necessarily affordable in many countries. A solution that involves the active participation of the population, on the other hand, has the potential to succeed in any country. The challenge is to attract and unite people to achieve such common goals. The theory of collective action and the Institutional Analysis and Development (IAD) framework, that have been originally used in resource management, are two concepts that can be adapted to organize recycling/recovery initiatives. This manuscript discusses what recycling/recovery programs can learn from the theory of collective action and the IAD framework, through a qualitative comparative study of such initiatives from three different cities. They are; Curitiba in Brazil, Padang in Indonesia, and Akure in Nigeria. The cases show the potential benefits of both concepts, not only in formulating and implementing recycling/recovery programs but also in making corrective measures for continuous improvements. All cases also showed the importance of increasing awareness-raising to change public perception towards waste from being a nuisance to a valuable resource.