bluuebunny/crossref_metadata_2025 huggingface dataset:
Campylobacter jejuni Infection, Anti-Ganglioside Antibodies, and Neuropathy
Authors: Latov Norman
Published: October 2022

Preceding infection with Campylobacter jejuni (Cj) occurs in approximately 30% of patients with Guillain–Barre syndrome (GBS), and the risk of GBS following Cj infection is increased by 77 to 100-fold. GBS is most often of the axonal subtype and is thought to be mediated by IgG antibodies to peripheral nerve gangliosides that are cross reactive with oligosaccharides in the Cj lipopolysaccharides (LPS). The antibodies are thought to be induced by molecular mimicry, where immune reactivity to a cross reactive epitope in the infectious organism and normal tissue can cause autoimmune disease. Clonally restricted IgM antibodies that react with the same oligosaccharides in gangliosides and Cj-LPS are associated with chronic neuropathies of otherwise similar phenotypes. The anti-ganglioside antibodies in GBS are of the IgG1 and IgG3 subclasses, indicating T-cell reactivity to the same antigens that could help disrupt the blood–nerve barrier. Cj infection can activate multiple innate and adoptive pro-inflammatory pathways that can overcome immune tolerance and induce autoimmunity. Elucidation of the specific immune mechanisms involved in the development of the autoantibodies and neuropathy would help our understanding of the relation between infection and autoimmunity and aid in the development of more effective preventive interventions and therapies.
Microbial Communities of Artisanal Fermented Milk Products from Russia
Authors: Kochetkova Tatiana V. , Grabarnik Ilya P. , Klyukina Alexandra A. , Zayulina Kseniya S. , Elizarov Ivan M. , Shestakova Oksana O. , Gavirova Liliya A. , Malysheva Anastasia D. , Shcherbakova Polina A. , Barkhutova Darima D. , Karnachuk Olga V. , Shestakov Andrey I. , Elcheninov Alexander G. , Kublanov Ilya V.
Published: October 2022

Fermented milk products (FMPs) have numerous health properties, making them an important part of our nutrient budget. Based on traditions, history and geography, there are different preferences and recipes for FMP preparation in distinct regions of the world and Russia in particular. A number of dairy products, both widely occurring and region-specific, were sampled in the households and local markets of the Caucasus republics, Buryatia, Altai, and the Far East and European regions of Russia. The examined FMPs were produced from cow, camel, mare’s or mixed milk, in the traditional way, without adding commercial starter cultures. Lactate and acetate were the major volatile fatty acids (VFA) of the studied FMPs, while succinate, formate, propionate and n-butyrate were present in lower concentrations. Bacterial communities analyzed by 16S rRNA gene V4 fragment amplicon sequencing showed that Firmicutes (Lactococcus, Lactobacillus, Streptococcus, Lentilactobacillus and Leuconostoc) was the predominant phylum in all analyzed FMPs, followed by Proteobacteria (Acetobacter, Klebsiella, Pseudomonas and Citrobacter). Lactobacillus (mainly in beverages) or Lactococcus (mainly in creamy and solid products) were the most abundant community-forming genera in FMPs where raw milk was used and fermentation took place at (or below) room temperature. In turn, representatives of Streptococcus genus dominated the FMPs made from melted or pasteurized milk and fermented at elevated temperatures (such as ryazhenka, cottage cheese and matsoni-like products). It was revealed that the microbial diversity of koumiss, shubat, ryazhenka, matsoni-like products, chegen, sour cream and bryndza varied slightly within each type and correlated well with the same products from other regions and countries. On the other hand, the microbiomes of kefir, prostokvasha, ayran, cottage cheese and suluguni-like cheese were more variable and were shaped by the influence of particular factors linked with regional differences and traditions expressed in specificities in the production process. The microbial diversity of aarts, khurunga, khuruud, tan, ayran and suluguni-like cheese was studied here, to our knowledge, for the first time. The results of this study emphasize the overall similarity of the microbial communities of various FMPs on the one hand, and specificities of regional products on the other. The latter are of particular value in the age of globalization when people have begun searching for new and unusual products and properties. Speaking more specifically, these novel products, with their characteristic communities, might be used for the development of novel microbial associations (i.e., starters) to produce novel products with improved or unique properties.
Psycho-Microbiology, a New Frontier for Probiotics: An Exploratory Overview
Authors: Accettulli Alessandra , Corbo Maria Rosaria , Sinigaglia Milena , Speranza Barbara , Campaniello Daniela , Racioppo Angela , Altieri Clelia , Bevilacqua Antonio
Published: October 2022

Probiotics are gradually gaining importance in the field of psychiatry in the form of psychobiotics. Psychobiotics’ studies examine the existing relationship between gut microbiota and mental phenomena; the intake of certain strains of probiotics, such as Bifidobacterium and Lactobacillus, for example, allow the gut microbial system to be modified in order to provide benefits at the psychic, immune, hormonal, and mental levels. Those who suffer from forms of depression, anxiety disorders, chronic stress, low mood, but also people who do not suffer from such disorders, can therefore benefit from the use of psychobiotics. Thanks to probiotics, neurochemicals can in fact be produced within the gut microbiota and interact with receptors of the enteric nervous system that innervate the entire gastrointestinal tract. Once they enter the portal circulation, these substances go on to influence components of the nervous system and ultimately the brain, through what is called the gut–brain axis. This article proposes an exploratory overview of the proven effects of probiotics on brain activity and psycho-related diseases, focusing on clinical studies and measurable outcomes. The search was conducted using two different online tools: ClinicalTrials.gov and PubMed.
Iron Compounds in Anaerobic Degradation of Petroleum Hydrocarbons: A Review
Authors: Castro Ana R. , Martins Gilberto , Salvador Andreia F. , Cavaleiro Ana J.
Published: October 2022

Waste and wastewater containing hydrocarbons are produced worldwide by various oil-based industries, whose activities also contribute to the occurrence of oil spills throughout the globe, causing severe environmental contamination. Anaerobic microorganisms with the ability to biodegrade petroleum hydrocarbons are important in the treatment of contaminated matrices, both in situ in deep subsurfaces, or ex situ in bioreactors. In the latter, part of the energetic value of these compounds can be recovered in the form of biogas. Anaerobic degradation of petroleum hydrocarbons can be improved by various iron compounds, but different iron species exert distinct effects. For example, Fe(III) can be used as an electron acceptor in microbial hydrocarbon degradation, zero-valent iron can donate electrons for enhanced methanogenesis, and conductive iron oxides may facilitate electron transfers in methanogenic processes. Iron compounds can also act as hydrocarbon adsorbents, or be involved in secondary abiotic reactions, overall promoting hydrocarbon biodegradation. These multiple roles of iron are comprehensively reviewed in this paper and linked to key functional microorganisms involved in these processes, to the underlying mechanisms, and to the main influential factors. Recent research progress, future perspectives, and remaining challenges on the application of iron-assisted anaerobic hydrocarbon degradation are highlighted.
Biocontrol Ability and Action Mechanism of Meyerozyma guilliermondii 37 on Soft Rot Control of Postharvest Kiwifruit
Authors: Pan Hui , Zhong Caihong , Wang Zupeng , Deng Lei , Li Wenyi , Zhao Juan , Long Chao-an , Li Li
Published: October 2022

Postharvest soft rot of kiwifruit has resulted in substantial market losses, yet there were few antagonistic yeasts reported to control the disease. This study screened 1113 yeast strains for potential antagonistic yeast to control soft rot of kiwifruit caused by Botryosphaeria dothidea and Diaporthe actinidiae, and strain 37 was selected to evaluate the control efficacy and mechanisms, which was identified as Meyerozyma guilliermondii via molecular biological identification. Our results showed that M. guilliermondii 37 effectively reduced pathogen spore germination rate to 28.52% and decay incidence of inoculated kiwifruit to 42.11% maximumly, whereas cell-free supernatant lacked antifungal activity, implying that M. guilliermondii 37 didn’t produce direct antifungal compounds against the two pathogens. In addition, M. guilliermondii 37 adhered tenaciously to the pathogens’ mycelium and colonized rapidly in kiwifruit flesh. Moreover, yeast strain 37 induced kiwifruit resistance by elevating the defense-related enzyme activity, increasing the antioxidant substances content, and suppressing the cell wall-degrading enzyme activity. Gene expression was consistent with the corresponding enzyme activity. Further postharvest yeast immersion treatment significantly reduced natural decay to 35.69% while maintaining soft-ripe quality. These results indicated that M. guilliermondii 37 might serve as a biocontrol agent against postharvest soft rot in kiwifruit.
Broad Antiviral Effects of Echinacea purpurea against SARS-CoV-2 Variants of Concern and Potential Mechanism of Action
Authors: Vimalanathan Selvarani , Shehata Mahmoud , Sadasivam Kannan , Delbue Serena , Dolci Maria , Pariani Elena , D’Alessandro Sarah , Pleschka Stephan
Published: October 2022

SARS-CoV-2 variants of concern (VOCs) represent an alarming threat as they show altered biological behavior and may escape vaccination effectiveness. Broad-spectrum antivirals could play an important role to control infections. The activity of Echinacea purpurea (Echinaforce® extract, EF) against (i) VOCs B1.1.7 (alpha), B.1.351.1 (beta), P.1 (gamma), B1.617.2 (delta), AV.1 (Scottish), B1.525 (eta), and B.1.1.529.BA1 (omicron); (ii) SARS-CoV-2 spike (S) protein-pseudotyped viral particles and reference strain OC43 as well as (iii) wild type SARS-CoV-2 (Hu-1) was analyzed. Molecular dynamics (MD) were applied to study the interaction of Echinacea’s phytochemical markers with known pharmacological viral and host cell targets. EF extract broadly inhibited the propagation of all investigated SARS-CoV-2 VOCs as well as the entry of SARS-CoV-2 pseudoparticles at EC50′s ranging from 3.62 to 12.03 µg/mL. The preventive addition of 25 µg/mL EF to epithelial cells significantly reduced sequential infection with SARS-CoV-2 (Hu-1) and OC43. MD analyses showed constant binding affinities to VOC-typical S protein variants for alkylamides, caftaric acid, and feruloyl-tartaric acid in EF extract and interactions with serine protease TMPRSS-2. EF extract demonstrated stable virucidal activity across seven tested VOCs, likely due to the constant affinity of the contained phytochemical substances to all spike variants. A possible interaction of EF with TMPRSS-2 partially would explain the cell protective benefits of the extract by the inhibition of membrane fusion and cell entry. EF may therefore offer a supportive addition to vaccination endeavors in the control of existing and future SARS-CoV-2 virus mutations.
Prevalence of Haemosporidian Parasites in an Arctic Breeding Seabird Species—The Red-Throated Diver (Gavia stellata)
Authors: Kleinschmidt Birgit , Dorsch Monika , Heinänen Stefan , Morkūnas Julius , Schumm Yvonne R. , Žydelis Ramūnas , Quillfeldt Petra
Published: October 2022

Haemosporida, vector-transmitted blood parasites, can have various effects and may also exert selection pressures on their hosts. In this study we analyse the presence of Haemosporida in a previously unstudied migratory seabird species, the red-throated diver Gavia stellata. Red-throated divers were sampled during winter and spring in the eastern German Bight (North Sea). We used molecular methods and data from a related tracking study to reveal (i) if red-throated divers are infected with Haemosporida of the genera Leucocytozoon, Plasmodium and Haemoproteus, and (ii) how infection and prevalence are linked with the breeding regions of infected individuals. Divers in this study were assigned to western Palearctic breeding grounds, namely Greenland, Svalbard, Norway and Arctic Russia. We found a prevalence of Leucocytozoon of 11.0% in all birds sampled (n = 45), of 33.0% in birds breeding in Norway (n = 3) and of 8.3% in birds breeding in Arctic Russia (n = 25). For two birds that were infected no breeding regions could be assigned. We identified two previously unknown lineages, one each of Plasmodium and Leucocytozoon. Haemosporida have not been detected in birds from Greenland (n = 2) and Svalbard (n = 2). In summary, this study presents the first record of Haemosporida in red-throated divers and reports a new lineage of each, Plasmodium and Leucocytozoon GAVSTE01 and GAVSTE02, respectively.
Removal of Hydrogen Sulfide from Swine-Waste Biogas on a Pilot Scale Using Immobilized Paracoccus versutus CM1
Authors: Kumdhitiahutsawakul Ladapa , Jirachaisakdeacha Dolruedee , Kantha Uthen , Pholchan Patiroop , Sattayawat Pachara , Chitov Thararat , Tragoolpua Yingmanee , Bovonsombut Sakunnee
Published: October 2022

Hydrogen sulfide (H2S) is a toxic and corrosive component that commonly occurs in biogas. In this study, H2S removal from swine-waste biogas using sulfur-oxidizing Paracoccus versutus CM1 immobilized in porous glass (PG) and polyurethane foam (PUF) biofilters was investigated. Bacterial compositions in the biofilters were also determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The biofilters were first tested on a laboratory scale under three space velocities (SV): 20, 30, and 40 h−1. Within 24 h, at an SV of 20 h−1, PG and PUF biofilters immobilized with P. versutus CM1 removed 99.5% and 99.7% of H2S, respectively, corresponding to the elimination capacities (EC) of 83.5 and 86.2 gm−3 h−1. On a pilot scale, with the horizontal PG-P. versutus CM1 biofilter operated at an SV of 30 h−1, a removal efficiency of 99.7% and a maximum EC of 113.7 gm−3 h−1 were achieved. No reduction in methane content in the outlet biogas was observed under these conditions. The PCR-DGGE analysis revealed that Paracoccus, Acidithiobacillus, and Thiomonas were the predominant bacterial genera in the biofilters, which might play important roles in H2S removal. This PG–P. versutus CM1 biofiltration system is highly efficient for H2S removal from swine-waste biogas.
Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments
Authors: Vitorino Inês Rosado , Klimek Dominika , Calusinska Magdalena , Lobo-da-Cunha Alexandre , Vasconcelos Vítor , Lage Olga Maria
Published: October 2022

The phylum Planctomycetota is known for having uncommon biological features. Recently, biotechnological applications of its members have started to be explored, namely in the genus Stieleria. Here, we formally describe a novel Stieleria isolate designated as strain ICT_E10.1T, obtained from sediments collected in the Tagus estuary (Portugal). Strain ICT_E10.1T is pink-pigmented, spherical to ovoid in shape, and 1.7 µm ± 0.3 x 1.4 µm ± 0.3 in size. Cells cluster strongly in aggregates or small chains, divide by budding, and have prominent fimbriae. Strain ICT_E10.1T is heterotrophic and aerobic. Growth occurs from 20 to 30 °C, from 0.5 to 3% (w/v) NaCl, and from pH 6.5 to 11.0. The analysis of the 16S rRNA gene sequence placed strain ICT_E10.1T into the genus Stieleria with Stieleria neptunia Enr13T as the closest validly described relative. The genome size is 9,813,311 bp and the DNA G+C content is 58.8 mol%. Morphological, physiological, and genomic analyses support the separation of this strain into a novel species, for which we propose the name Stieleria sedimenti represented by strain ICT_E10.1T as the type of strain (=CECT 30514T= DSM 113784T). Furthermore, this isolate showed biotechnological potential by displaying relevant biosynthetic gene clusters and potent activity against Staphylococcus aureus.
Sporotrichosis: A Review of a Neglected Disease in the Last 50 Years in Brazil
Authors: Alvarez Carmen Magaly , Oliveira Manoel Marques Evangelista , Pires Regina Helena
Published: October 2022

Sporotrichosis is caused by fungi belonging to the genus Sporothrix, which saprophytically are found in plants and organic matter. However, cats are highly susceptible to contamination with fungal spores and, when they become sick, they can transmit it to other animals and to man. The objective of this study is to carry out a systematic review on the emergency, diagnosis, clinical symptoms, therapeutics, and control of zoonotic sporotrichosis. Published data covering the last 50 years using a combination of keywords were selected to answer the question: Why has the zoonotic sporotrichosis been a neglected disease up to now? A total of 135 studies were included in this review. The studies emphasize that in recent decades, Brazil has experienced an unprecedented zoonotic outbreak of sporotrichosis. Advances on the genus Sporothrix allowed one to associate thermotolerance, capacity for melanin synthesis, potential for adhesion to tissue macromolecules, ergosterol peroxide production, and expression of virulence proteins as tools for infection and invasion in S. brasiliensis, the main species involved, although cases with S. schenckii or S. lurei were also reported. Correct diagnosis, early treatment, basic educational measures that emphasize responsible ownership of animals and reproductive control programs for felines can contribute to the control of zoonosis.
The Effect of Physical Separation and Calcination on Enrichment and Recovery of Critical Elements from Coal Gangue
Authors: Zhang Lei , Chen Hangchao , Pan Jinhe , Wen Zhiping , Shi Shulan , Long Xin , Zhou Changchun
Published: October 2022

Critical metallic elements in coal gangue have great utilization potential, especially due to the current shortage of these metals. This paper focused on examining the feasibility of physical separation (screening and float-sink tests) and calcination treatment for the enrichment of critical elements (Li, Ga, and rare earth elements plus yttrium (REY)) from coal gangue. The impacts of these enrichment methods on the acid leaching recovery of these elements were then studied. Screening tests indicated that Li and Ga were enriched in >0.125 mm size fraction and the content of REY was highest in <75 μm size fraction. Float-sink tests showed that high-density fractions were enriched in Li and Ga, and low-density fractions were enriched in REY. Physical separation cannot significantly improve the leaching rate of Li, Ga, and REY. Notably, Li, Ga, and REY were enriched significantly, and their acid leaching recoveries were increased by 54~68% after calcination under 400 °C. Sequential chemical extraction tests showed that the majority of insoluble Li, Ga, and REY was converted into soluble forms at the above temperature, which is attributed to the formation of amorphous metakaolinite and the decomposition of organic matter. Based on the results, a conceptually combined flowsheet was proposed for the extraction of Li and Ga from coal gangue.
Diagenesis and Reservoir Evolution Model of the Ediacaran Dengying Formation in the Sichuan Basin: Evidence from Laser Ablation U-Pb Dating and In Situ Isotope Analysis
Authors: Qiao Zhanfeng , Dong Jianhua , Yu Zhou , Li Wenzheng , Wang Xiaofang , Jiang Lei , Qing Hairuo
Published: October 2022

The microbial dolomite of the Ediacaran Dengying Fm., Sichuan Basin, SW China is endowed with abundant oil and gas resources. A complex diagenetic history greatly complicates the development of such microbial dolomite reservoirs, and has severely restrained hydrocarbon exploration. This study focused on key field profiles in Eastern Sichuan and aimed to decipher the origin and evolution of dolomites, using novel techniques, including elemental mapping, laser ablation U-Pb dating as well as carbon, oxygen, and strontium isotope analyses. Additionally, we compared the diagenesis and reservoir development model for the Dengying microbial dolomites of the Central Sichuan to other areas, with the aim to provide practical guidance for oil and gas exploration across the entire Sichuan Basin. Our results have shown that the Ediacaran microbial dolomite in Eastern Sichuan experienced four stages of diagenetic modification–specifically: (1) syn-sedimentary dolomitization; (2) penecontemporaneous cementation of fibrous dolomite cement; (3) bladed dolomite and brown-dark very fine crystalline dolomite cementation during the Ordovician; and (4) fine crystalline dolomite and medium-coarse crystalline dolomite cementation during the Silurian-Devonian. Petrology and geochemistry data imply that each diagenetic phase of dolomitization was mainly sourced from marine-dominated fluids. The influence of tectonically related fluids was rarely noted. Significantly, U-Pb ages of these dolomites reveal that the history of dolomite infill to pores within the Eastern Sichuan Basin clearly post-dated that in the Central Sichuan region, resulting in high porosities (6%–11%) that favored hydrocarbon accumulation. This study suggests that the platform margin at the eastern edge of the Xuanhan-Kaijiang paleo-uplift is favorable as the next key exploration target for microbial dolomites in the Sichuan Basin.
Bioleaching Test of Polymetallic Nodule Samples from the IOM Exploration Area
Authors: Štyriaková Darina , Štyriaková Iveta , Šuba Jaroslav , Baláž Peter , Abramowski Tomasz
Published: October 2022

The Interoceanmetal Joint Organization’s (IOM) exploration license is granted in the area located within the Clarion–Clipperton Zone of the Eastern Central Pacific. All activities related to exploration of minerals in this area are beyond the limits of national jurisdiction and come under the provision of the United Nations Convention on the Law of the Sea and related regulations. The geological survey, which was the subject of the exploration, focused on the polymetallic nodule deposits. Other activities covered the tests of nodule processing, including alternative methods of metal extraction. This article describes the methodology of the bioleaching process and provides a summary of the initial results of the bioleaching test, applied to the samples of polymetallic nodules taken from the H22 exploration block of the IOM exploration area. The bioleaching efficiency under different pulp density conditions was studied. The enrichment of heterotrophic Mn-reducing microorganisms was prepared as the leaching medium. Bioleaching of the samples took place with different sample weights in order to determine the maximum possible extraction yield and the highest concentration of elements obtained in the leachate. During the bioleaching, the media were also exchanged to achieve maximum polymetallic nodule decomposition with regular addition of nutrients for heterotrophic Mn-reducing bacteria. The bioleaching efficiency of the polymetallic nodules increased to 100% (without Cu) when the pulp density was reduced to 0.1% (w/v). The discontinuous bioleaching and washing process recovered 55% Mn, 36% Ni, 15% Co, 27% Zn, and 38% Cu when the pulp density was increased to 10% (w/v). To achieve total Mn, Cu, Zn, Co, and Ni recovery from the polymetallic nodules, an intermittent bioleaching with about 25 changes of 60% of the medium was required when the pulp density was increased to 10% (w/v). Bioleaching has the potential to be used as an environmentally friendly alternative to other commonly used options for leaching and processing.
Late Mesozoic Granitoid Magmatism in the Evolution of the Eastern Flank of the Mongol-Okhotsk Orogenic Belt (Russia)
Authors: Derbeko Inna M.
Published: October 2022

In this article, for the first time, a comparison is made of magmatic events that occurred in the northern and southern framing of the eastern flank of the Mongol-Okhotsk orogenic belt. It is established that these events occurred simultaneously. The igneous rocks accompanying these events are identical in their material characteristics. And their geochemical characteristics reflect the geodynamic processes that took place in the frame of the Mongolian-Okhotsk orogenic belt at the end of the Mesozoic. Igneous rocks are represented by a wide range of rocks: from plutonic to volcanic. The range of their material composition is also wide. But the main component belongs to granitoids. At the initial stage of the Late Mesozoic geological events, granitoids of the adakitic series (149–138 Ma) were formed. Then formations of the calc-alkaline series (140–122 Ma) begin to form. However, the rocks of both the first and second stages were formed under suprasubduction conditions. The difference in the composition of these formations is established by the values of Sr-Nd isotopes. This reflects their spatial affiliation. In the northern frame, they break through the widely developed Archean and Proterozoic formations, and in the southern frame, only Proterozoic ones.
Order–Disorder Diversity of the Solid State by NMR: The Role of Electrical Charges
Authors: Sánchez-Muñoz Luis , Florian Pierre , Gan Zhehong , Muñoz Francisco
Published: October 2022

The physical explanations and understanding of the order–disorder phenomena in the solid state are commonly inferred from the experimental capabilities of the characterization techniques. Periodicity is recorded according to the averaging procedure of the conventional reciprocal-space techniques (RSTs) in many solids. This approach gives rise to a sharp trimodal view including non-crystalline or amorphous compounds, aperiodic crystals and periodic crystals. However, nuclear magnetic resonance (NMR) spectroscopy offers an alternative approach that is derived from the distinct character of the measurements involved at the local scale. Here, we present a sequence of progressive order–disorder states, from amorphous structures up to fully ordered mineral structures, showing the great diversity existing in the solid state using multinuclear NMR spectroscopy. Some examples in glasses and products of their crystallization are used, as well as several minerals (including beryl-group and feldspar-group minerals) at magnetic fields up to 35.2 T, and some examples from literature. This approach suggests that the solid state is a dynamic medium, whose behavior is due to atomic adjustments from local compensation of electrical charges between similar structural states, which explains Ostwald’s step rule of successive reactions. In fully ordered feldspar minerals, we propose that the electronic structure of the elements of the cavity site is involved in bonding, site morphology and feldspar topology. Furthermore, some implications are derived about what is a mineral structure from the point of view of the NMR experiments. They open the possibility for the development of the science of NMR Mineralogy.
Anisotropy of Out-of-Phase Magnetic Susceptibility: A Non-Standard Approach for Magnetic Subfabrics Determination in Variscan Granites of Iberian Massif
Authors: Cruz Cláudia , Sant’Ovaia Helena , McCarthy William , Noronha Fernando
Published: October 2022

The magnetic susceptibility measured in an alternating field is made up of in-phase and out-of-phase components. The in-phase Anisotropy of Magnetic Susceptibility (ipAMS) measures the bulk response of all minerals in a sample; however, out-of-phase AMS (opAMS) is sensitive to only select ferromagnetic minerals such as hematite, titanomagnetite, and ultrafine magnetite. The opAMS can be harnessed as a tool for the direct determination of magnetic subfabrics defined by ferromagnetic minerals. This work focuses on the following three Portuguese plutons: Lamas de Olo, Lavadores-Madalena, and Santa Eulália. The results show that the magnetic susceptibility is lower in opAMS, the degree of magnetic anisotropy is much higher in the opAMS, and the ellipsoid shape parameter has no significant differences. The ipAMS and opAMS tensors are, in general, coaxial, which indicates that the standard AMS fabric is parallel to the subfabric of minerals such as hematite, titanomagnetite, and ultrafine magnetite.
Methodology and Model to Predict HPGR Throughput Based on Piston Press Testing
Authors: Pamparana Giovanni , Klein Bern , Bergerman Mauricio Guimaraes
Published: October 2022

Sizing High-Pressure Grinding Rolls (HPGR) requires a large quantity of material, making it not attractive and costly to be considered for new mining projects regardless of their energy consumption reduction benefits. Ongoing efforts are being made at the University of British Columbia to predict the behaviour of the HPGR using a low quantity of material on a piston-and-die press apparatus. Although the energy requirements and size reduction predictive models are already developed, there is still a need to predict the HPGR throughput on a small-scale test. This paper presents a new model to predict the HPGR throughput based on the previously developed model to predict the operational gap by using less than 2 kg of sample. The throughput model was developed using machine learning techniques and calibrated using pilot-scale HPGR tests and piston press tests. The resulting model has an R2 of 0.91 with an average prediction error of ±4.2%. The developed methodology has the potential to fill the gap of the missing throughput model. Further pilot-scale HPGR testing is required to continue validating the model.
Study of the Reaction Mechanisms during the Thermal Decomposition of Arsenic Sulfide (V) at High Temperatures under Non-Isothermal Conditions
Authors: Castro Kristhobal , Balladares Eduardo , Jerez Oscar , Pérez-Tello Manuel , Aracena Álvaro
Published: October 2022

The reaction mechanisms during thermal decomposition in As-S systems are complex to describe due to the physicochemical characteristics of arsenic and its sulfides. Non-isothermal tests were carried out in a NETZSCH DTA/TG thermal analysis equipment, and interrupted tests were carried out in a vertical oven adapted for this purpose; both tests were carried out in an inert nitrogen atmosphere. The information obtained experimentally was analyzed and correlated to determine the phases and reaction mechanisms during the thermal decomposition of an arsenic sulfide (V) sample. As a result of this study, the mechanism of thermal decomposition of an arsenic sulfide sample was obtained and the apparent activation energy was determined by the Kissinger and Ozawa methods.
Experimental Study on the Effect of Mineral Composition on Shrinkage Fractures: Implications for the Origin of the Diagenetic Shrinkage Fractures in Marine–Continental Transitional Shales
Authors: Zheng Guo , Zhang Jinchuan , Liu Yang , Huo Zhipeng , Xu Longfei
Published: October 2022

Natural fractures in shale have been commonly recognized as a vital factor in shale gas production. Diagenetic shrinkage fracture is an important type of natural fracture; however, its formation mechanism and prediction remain largely unexplored. Given the similarity between diagenetic shrinkage fractures and desiccation cracks, the influence of smectite, kaolin, quartz, and feldspar on shrinkage fractures were investigated using the physical simulation experiment of desiccation in this study. Here, water evaporation, surface cracks initiation and propagation processes were monitored during the whole desiccation. The morphological characteristics of cracks patterns were quantitatively described. Diagenetic shrinkage fractures of transitional shale with different minerals were observed using SEM. The results show that the development and morphology of cracks are affected by the mineral composition, and the sediments with higher clay content tend to form more complex fracture networks. We further propose a morphological prediction model for cracks and compare this model with diagenetic shrinkage fractures under SEM. We found that the effects of mineral composition on both were similar, with more well-developed diagenetic shrinkage fractures in those clay-rich samples. Taken together, this study implies the feasibility of using physical simulation experiment of desiccation cracks to invert diagenetic shrinkage fractures and provides new insights into the mechanism and development regularity of diagenetic shrinkage fractures.
3D Mineral Prospectivity Mapping of Zaozigou Gold Deposit, West Qinling, China: Deep Learning-Based Mineral Prediction
Authors: Yu Zhengbo , Liu Bingli , Xie Miao , Wu Yixiao , Kong Yunhui , Li Cheng , Chen Guodong , Gao Yaxin , Zha Shuai , Zhang Hanyuan , Wang Lu , Tang Rui
Published: October 2022

This paper focuses on the scientific problem of quantitative mineralization prediction at large depth in the Zaozigou gold deposit, west Qinling, China. Five geological and geochemical indicators are used to establish geological and geochemical quantitative prediction model. Machine learning and Deep learning algorithms are employed for 3D Mineral Prospectivity Mapping (MPM). Especially, the Student Teacher Ore-induced Anomaly Detection (STOAD) model is proposed based on the knowledge distillation (KD) idea combined with Deep Auto-encoder (DAE) network model. Compared to DAE, STOAD uses three outputs for anomaly detection and can make full use of information from multiple levels of data for greater overall robustness. The results show that the quantitative mineral resources prediction by applying the STOAD model has a good performance, where the value of Area Under Curve (AUC) is 0.97. Finally, three main mineral exploration targets are delineated for further investigation.
Comparative Study of the Mineralogy and Chemistry Properties of Elephant Bones: Implications during Diagenesis Processes
Authors: Monasterio-Guillot Luis , Crespo-López Laura , Rodríguez Navarro Alejandro B. , Álvarez-Lloret Pedro
Published: October 2022

Elephants are currently the largest terrestrial mammals, along with their extinct ancestor the mammoth. These species are of great interest due to their geographic distribution and ecological importance. However, the bone mineral characteristics of their skeleton as well as their alteration during burial processes over millions of years have been poorly investigated. In the current research, we analyzed the compositional and structural mineral properties of different types (i.e., long and flat bones) of elephant and mammoth bones. For this purpose, we performed a comparative study between these bone types using complementary analytical techniques based on X-ray diffraction, thermogravimetry, Fourier transform infrared spectroscopy, scanning electron microscopy, and inductively coupled plasma spectroscopic analysis. The chemical composition of the samples shows a relative loss of a considerable percentage of water and organic matter components for the fossilized bone, accompanied by a higher bone mineralization degree and the presence of other crystalline phases (i.e., calcite and pyrite) related to a certain degree of diagenesis alteration. Our results also show a variation in the crystalline properties of mammoth bone related to the recrystallization process during the dissolution–precipitation transformation through diagenetic burial. This research provides relevant information for understanding the mineral properties of different types of bones and their possible changes during diagenesis.
Radiological Risk Parameters of the Phosphorite Deposits, Gebel Qulu El Sabaya: Natural Radioactivity and Geochemical Characteristics
Authors: Lasheen El Saeed R. , Zakaly Hesham M. H. , Alotaibi B. M. , Saadawi Diaa A. , Ene Antoaneta , Fathy Douaa , Awad Hamdy A. , El Attar Raafat M.
Published: October 2022

This study investigates the distribution of natural radioactivity and geological, geochemical, and environmental risk assessments of phosphorite deposits to determine their suitability for international applications (such as phosphoric acid and phosphatic fertilizers). The examined Late Cretaceous phosphorite deposits belong to the Duwi Formation, which is well exposed on the southern scarp boundary at the central part of Abu Tartur Plateau, Gebel Qulu El Sabaya, East Dakhla Oasis. This formation is classified into lower phosphorite, middle shale, and upper phosphorite members. The lower phosphorite ranges in thickness from 2 to 3.5 m and mainly comprises apatite (possibly francolite), dolomite, calcite, quartz, hematite, anhydrite, and kaolinite. They contain an average concentration of CaO (38.35 wt.%), P2O5 (24.92 wt.%), SiO2 (7.19 wt.%), Fe2O3 (4.18 wt.%), MgO (3.99 wt.%), F (1.59 wt.%), Al2O3 (1.84 wt.%), Na2O (1.33 wt.%), and K2O (0.22 wt.%). Natural radioactivity and radiological parameters were investigated for fifteen samples of phosphorites using a NaI (Tl) scintillation detector. Absorbed dose rates, outdoor and indoor annual effective dose, radium equivalent activity, external and internal hazard, and excess cancer risk values are higher than the recommended levels, reflecting that exposure to these deposits for a long time may lead to health risks to human organs.
Geochronology of the Baishi W-Cu Deposit in Jiangxi Province and Its Geological Significance
Authors: Li Li , Li Hai-Li , Wang Guo-Guang , Sun Jian-Dong
Published: October 2022

The Baishi W-Cu deposit is located in the Nanling metallogenic belt, which is famous for its numerous W deposits and reserves. The formation age of this deposit remains unclear. In order to further infer the formation age of the deposit, this study conducted detailed LA-ICP-MS U-Pb isotopic analyses of zircon and monazite selected from ore-related Baishi granite. The LA-ICP-MS zircon U-Pb weighted average ages of Baishi granite were determined to be 223 ± 2 Ma and 226 ± 1 Ma, and the LA-ICP-MS U-Pb weighted average ages of monazite were determined to be 224 ± 2 Ma and 223 ± 1 Ma. The BSE image of monazite was homogeneous, and the pattern of rare earth elements had an obvious negative Eu anomaly, indicating that monazite was of magmatic origin. Combining the ages of zircon and monazite, this study inferred that Baishi granite and the Baishi W-Cu deposit formed in the Triassic. The determination of the ore-forming event of the Baishi W-Cu deposit provides new data regarding the important Indosinian (Triassic) mineralization events in the Nanling metallogenic belt and suggests that geologists should strengthen the prospecting work of Indosinian tungsten deposits in the Nanling area. In terms of tectonic setting, it was inferred that the Triassic Baishi W-Cu deposit was formed in the extensional environment after intracontinental orogeny.
The Role of Hypoxia-Inducible Factor-1 Alpha in Renal Disease
Authors: Liu Huixia , Li Yujuan , Xiong Jing
Published: October 2022

Partial pressure of oxygen (pO2) in the kidney is maintained at a relatively stable level by a unique and complex functional interplay between renal blood flow, glomerular filtration rate (GFR), oxygen consumption, and arteriovenous oxygen shunting. The vulnerability of this interaction renders the kidney vulnerable to hypoxic injury, leading to different renal diseases. Hypoxia has long been recognized as an important factor in the pathogenesis of acute kidney injury (AKI), especially renal ischemia/reperfusion injury. Accumulating evidence suggests that hypoxia also plays an important role in the pathogenesis and progression of chronic kidney disease (CKD) and CKD-related complications, such as anemia, cardiovascular events, and sarcopenia. In addition, renal cancer is linked to the deregulation of hypoxia pathways. Renal cancer utilizes various molecular pathways to respond and adapt to changes in renal oxygenation. Particularly, hypoxia-inducible factor (HIF) (including HIF-1, 2, 3) has been shown to be activated in renal disease and plays a major role in the protective response to hypoxia. HIF-1 is a heterodimer that is composed of an oxygen-regulated HIF-1α subunit and a constitutively expressed HIF-1β subunit. In renal diseases, the critical characteristic of HIF-1α is protective, but it also has a negative effect, such as in sarcopenia. This review summarizes the mechanisms of HIF-1α regulation in renal disease.
First Evidence of Gastroprotection by Schinus molle: Roles of Nitric Oxide, Prostaglandins, and Sulfhydryls Groups in Its Mechanism of Action
Authors: Sánchez-Mendoza María Elena , López-Lorenzo Yaraset , Cruz-Antonio Leticia , Arrieta-Baez Daniel , Pérez-González Miranda Carolina , Arrieta Jesús
Published: October 2022

Schinus molle is a plant traditionally used in Mexico to treat gastric disorders. However, no scientific evidence has been reported on its gastroprotective effect. The aim of the current contribution was to conduct a bioassay-guided study on S. molle to evaluate its gastroprotective activity in a model of Wistar rats given ethanol orally to induce gastric lesions. The hexane and dichloromethane extracts from the tested plant showed over 99% gastroprotection at a dose of 100 mg/kg. From the hexane extract, two of the three fractions (F1 and F2) afforded over 99% gastroprotection. The F1 fraction was subjected to column chromatography, which revealed a white solid. Based on the ESI-MS analysis, the two main compounds in this solid were identified. The predominant compound was probably a triterpene. This mixture of compounds furnished about 67% gastroprotection at a dose of 100 mg/kg. Pretreatment with L-NAME, indomethacin, and NEM was carried out to explore the possible involvement of nitric oxide, prostaglandins, and/or sulfhydryl groups, respectively, in the gastroprotective activity of the white solid. We found evidence for the participation of all three factors. No antisecretory activity was detected (tested by pylorus ligation). In conclusion, evidence is herein provided for the first time of the gastroprotective effect of S. molle.
Electrocatalytic Behavior of an Amide Functionalized Mn(II) Coordination Polymer on ORR, OER and HER
Authors: Paul Anup , Radinović Kristina , Hazra Susanta , Mladenović Dušan , Šljukić Biljana , Khan Rais Ahmad , Guedes da Silva Maria Fátima C. , Pombeiro Armando J. L.
Published: October 2022

The new 3D coordination polymer (CP) [Mn(L)(HCOO)]n (Mn-CP) [L = 4-(pyridin-4-ylcarbamoyl)benzoate] was synthesised via a hydrothermal reaction using the pyridyl amide functionalized benzoic acid HL. It was characterized by elemental, FT-IR spectroscopy, single-crystal and powder X-ray diffraction (PXRD) analyses. Its structural features were disclosed by single-crystal X-ray diffraction analysis, which revealed a 3D structure with the monoclinic space group P21/c. Its performance as an electrocatalyst for oxygen reduction (ORR), oxygen evolution (OER), and hydrogen evolution (HER) reactions was tested in both acidic (0.5 M H2SO4) and alkaline (0.1 M KOH) media. A distinct reduction peak was observed at 0.53 V vs. RHE in 0.1 M KOH, which corresponds to the oxygen reduction, thus clearly demonstrating the material’s activity for the ORR. Tafel analysis revealed a Tafel slope of 101 mV dec−1 with mixed kinetics of 2e− and 4e− pathways indicated by the Koutecky–Levich analysis. Conversely, the ORR peak was not present in 0.5 M H2SO4 indicating no activity of Mn-CP for this reaction in acidic media. In addition, Mn-CP demonstrated a noteworthy activity toward OER and HER in acidic media, in contrast to what was observed in 0.1 M KOH.
Lentinus edodes Polysaccharides Alleviate Acute Lung Injury by Inhibiting Oxidative Stress and Inflammation
Authors: Zhang Yiwen , Cui Yanfei , Feng Yanbo , Jiao Fengping , Jia Le
Published: October 2022

Acute lung injury (ALI) is a kind of lung disease with acute dyspnea, pulmonary inflammation, respiratory distress, and non-cardiogenic pulmonary edema, accompanied by the mid- and end-stage characteristics of COVID-19, clinically. It is imperative to find non-toxic natural substances on preventing ALI and its complications. The animal experiments demonstrated that Lentinus edodes polysaccharides (PLE) had a potential role in alleviating ALI by inhibiting oxidative stress and inflammation, which was manifested by reducing the levels of serum lung injury indicators (C3, hs-CRP, and GGT), reducing the levels of inflammatory factors (TNF-α, IL-1β, and IL-6), and increasing the activities of antioxidant enzymes (SOD and CAT) in the lung. Furthermore, PLE had the typical characteristics of pyran-type linked by β-type glycosidic linkages. The conclusions indicated that PLE could be used as functional foods and natural drugs in preventing ALI.
Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon
Authors: Xavier Júlia Karla A. M. , Baia Talissa Gabriele C. , Alegria Oscar Victor C. , Figueiredo Pablo Luis B. , Carneiro Adriana R. , Moreira Edith Cibelle de O. , Maia José Guilherme S. , Setzer William N. , da Silva Joyce Kelly R.
Published: October 2022

Cinnamomum verum (Lauraceae), also known as “true cinnamon” or “Ceylon cinnamon” has been widely used in traditional folk medicine and cuisine for a long time. The systematics of C. verum presents some difficulties due to genetic variation and morphological similarity between other Cinnamomum species. The present work aimed to find chemical and molecular markers of C. verum samples from the Amazon region of Brazil. The leaf EOs and the genetic material (DNA) were extracted from samples cultivated and commercial samples. The chemical composition of the essential oils from samples of C. verum cultivated (Cve1-Cve5) and commercial (Cve6-c-Cv9-c) was grouped by multivariate statistical analysis of Principal Component Analysis (PCA). The major compounds were rich in benzenoids and phenylpropanoids, such as eugenol (0.7–91.0%), benzyl benzoate (0.28–76.51%), (E)-cinnamyl acetate (0.36–32.1%), and (E)-cinnamaldehyde (1.0–19.73%). DNA barcodes were developed for phylogenetic analysis using the chloroplastic regions of the matK and rbcL genes, and psbA-trnH intergenic spacer. The psbA-trnH sequences provided greater diversity of nucleotides, and matK confirmed the identity of C. verum. The combination of DNA barcode and volatile profile was found to be an important tool for the discrimination of C. verum varieties and to examine the authenticity of industrial sources.