bluuebunny/crossref_metadata_2025 huggingface dataset:
The impact of coffee consumption on osteoarthritis: insights from NHANES and Mendelian randomization analysis
Authors: Feng Kai , Li Peng , Guo Haohui , Chen Zhirong
Published: December 2024

Background

Osteoarthritis (OA) is a prevalent degenerative joint condition, and emerging evidence suggests that dietary factors, such as coffee consumption, may influence its risk. However, the relationship between coffee consumption and the risk of developing OA remains ambiguous. This study aims to explore the association between coffee intake and OA complemented by Mendelian randomization (MR) to infer causality.

Materials and methods

We analyzed data from 32,439 participants across 10 NHANES cycles (1999–2018), including 3,676 individuals diagnosed with OA. Osteoarthritis was diagnosed through a structured questionnaire, while coffee consumption was assessed via 24-h dietary recalls. Participants were categorized based on reported coffee intake: 0 cups, <2 cups, 2–4 cups, and >4 cups per day. We employed weighted multivariable logistic regression to examine associations between coffee consumption and OA by using data from the NHANES 1999–2018, adjusting for various covariates. Subsequently, a MR analysis was conducted using genetic variants as instrumental variables to infer causal relationships, with multiple methods including inverse-variance weighted (IVW) analysis, MR-Egger regression, and weighted median techniques to assess the robustness, heterogeneity, and potential pleiotropy of our findings.

Results

Our regression models indicated an increased risk of OA with rising coffee consumption, with significant associations noted particularly for those consuming more than 4 cups daily (OR = 1.19, 95% CI: 1.00–1.41, p = 0.049). In MR analysis, coffee intake was causally linked to OA types, demonstrating increased risk for knee OA (KOA: OR = 1.60, 95% CI: 1.08–2.35, p = 0.018), hip OA (HOA: OR = 1.85, 95% CI: 1.06–3.25, p = 0.031), and combined KOA and HOA (KHOA: OR = 1.66, 95% CI: 1.18–2.33, p = 0.003). Sensitivity analyses confirmed the stability of results across multiple evaluation methods.

Conclusion

Our findings highlight a significant association between coffee consumption and an increased risk of OA, suggesting that higher intake levels may contribute to OA morbidity. These results warrant further exploration into the underlying biological mechanisms and implications for dietary guidelines in populations at risk for OA.
Enhancing iron and zinc bioavailability in maize (Zea mays) through phytate reduction: the impact of fermentation alone and in combination with soaking and germination
Authors: Nsabimana Samuel , Ismail Tariq , Lazarte Claudia E.
Published: December 2024

Introduction

Phytates are nutrient-binding compounds found mainly in cereals and legumes, which may significantly contribute to micronutrient malnutrition in regions where phytate-rich cereals, such as maize, are staple food.

Objectives

This study investigated how maize fermentation, both alone and in combination with soaking and germination, can reduce phytate levels and enhance the estimated bioavailability of iron and zinc.

Methods

We evaluated various fermentation methods, including spontaneous fermentation; fermentation with starter cultures, either Lactiplantibacillus plantarum 299v® (Lp299) or yogurt containing viable Lacticaseibacillus casei; and fermentation with Lp299 of soaked and germinated maize. The outcome variables included changes in pH and lactic acid content during fermentation, and measurements of phytate levels (spectrophotometry), minerals (Atomic absorption) and protein (protein analyzer) in maize samples before and after treatments.

Results

Fermentation with Lp299 of soaked and germinated maize grains yielded a phytate reduction of up to 85.6% decreasing from 9.58 ± 0.05 g·kg−1 in raw maize to 1.39 ± 0.09 g·kg−1 after processing. Fermentation of raw maize flour using Lp299 or yogurt resulted in a similar phytate reduction of 65.3% (3.35 ± 0.26 g·kg−1) and 68.7% (3.02 ± 0.01 g·kg−1) respectively. Spontaneous fermentation yielded a phytate reduction of 51.8% (4.65 ± 0.40 g·kg−1). This reduction in phytate content enhanced the estimated bioavailability of iron and zinc, particularly in the soaking-germination-fermentation combination, where the Phytate:Zinc molar ratio (Phy:Zn) dropped from 40.76 to 7.77, representing 81% reduction from the raw maize. The Phytate:Iron molar ratio (Phy:Fe) dropped from 41.42 to 6.24 indicating an 85% reduction. Additionally, fermentation led to a significant increase (p = 0.001) in protein content in maize flour after fermentation, ranging from 7.3 to 10.3% after the various fermentation treatments. There was not significant difference in the protein increase when compared the fermentation types.

Conclusion

Lactic acid fermentation of soaked and germinated maize grains, emerged as the most promising process to enhance the bioavailability of essential minerals. This approach could help alleviate mineral deficiencies in populations dependent on maize-based diets. The findings underscore the potential of fermentation to be applied at the household level, which may bring up an alternative for programs and policies focused on reducing micronutrient deficiencies and improving food security in developing regions.
Association between serum uric acid and all-cause and cardiovascular-related mortality in hemodialysis patients
Authors: Gan Wenyuan , Zhu Fan , Fang Xun , Wang Wenzhe , Shao Danni , Mao Huihui , Xiao Wei , Chen Wenli , Xu Fang , Zeng Xingruo
Published: December 2024

Background

The association between serum uric acid (UA) and all-cause and cardiovascular-related mortality in hemodialysis (HD) patients is conflicting. We investigated this association and explored the effect modification of underlying nutritional status, as reflected in the lean tissue index (LTI) and the Geriatric Nutritional Risk Index (GNRI), which serve as markers of muscle mass and nutritional risk in HD patients.

Methods

A retrospective cohort study was conducted from January 2019 to December 2023. We investigated the association between serum UA and the outcomes using the Cox proportional hazards regression and restricted cubic splines. Subgroup analyses based on the LTI and GNRI were conducted to explore possible effect modification.

Results

During a mean follow-up of 32.9 months, 876 patients who underwent HD were included in the analysis. The association between serum UA and all-cause mortality showed a non-linear U-shaped pattern (p = 0.007), with a survival benefit observed for the patients with serum UA levels between 3.4 and 6.8 mg/dL. In the multivariable-adjusted model, the low and high UA groups were associated with a greater risk of all-cause mortality compared to the reference UA group (hazard ratio (HR) =1.24, confidence interval (CI) 1.03–2.12, p = 0.027; HR = 1.09; CI 1.05–2.08. p = 0.012). In the low UA group, a greater risk of mortality was observed in patients with low LTI (<12.3; HR 1.56, 95% CI 1.22–1.82) and GNRI values (<102.1; HR 1.43, 95% CI 1.12–1.76), but not in those with high LTI and GNRI values. There was no significant association between serum UA and cardiovascular disease-related mortality.

Conclusion

Our study showed that lower and higher serum UA levels increase the risk of all-cause mortality in HD patients. Among the patients with lower UA levels, low LTI and GNRI values showed a greater risk of mortality. This finding suggested that better nutritional status, rather than elevated UA levels, is likely to improve long-term survival in HD patients.
Progress of research on PD-L1 inhibitor adebrelimab usage in malignant tumors
Authors: Cheng Pan , He Jichen , Cheng Pingping , Chen Kaixia , Zhao Guangyu
Published: December 2024

Adebrelimab is a humanized monoclonal antibody against programmed death-ligand 1 (PD-L1) and is also a novel immune checkpoint inhibitor, which has been used in the first-line treatment of extensive stage small cell lung cancer (SCLC) with its unique mechanism of action and good clinical efficacy. Significant progress has been made in the treatment of adebrelimab in other malignancies such as non-small cell lung cancer, triple-negative breast cancer, esophageal squamous cell carcinoma, and the treatment of SCLC at different stages is also being explored. Therefore, adebrelimab emerges as a promising new treatment option for patients with small cell lung cancer (SCLC) and other types of malignant tumors.
A case report of PGAP2-related hyperphosphatasia with impaired intellectual development syndrome in a Chinese family and literature review
Authors: Pan Yijun , Ren Bin , Chen Lijuan , Li Qiang
Published: December 2024

Recently, mutations have been identified in six genes (PIGA, PIGY, PIGO, PGAP2, PIGW and PGAP3) encoding proteins in the Glycosyl phosphatidylinositol(GPI)-anchor-synthesis pathway in individuals with hyperphosphatasia with impaired intellectual development syndrome(HPMRS). Reports involving the rare pathogenic gene, post-GPI attachment to proteins 2 (PGAP2) are quite limited. In this study, we reported two patients with PGAP2 variants related neurodevelopmental disorders from Asian population. The proband, onset of epileptic spasms at 5 months, concurrently with global developmental dalay, facial malformation and elevated alkaline phosphatase. His younger sister, onset of epileptic spasms at 2 months, having similar clinical features as the proband. Their phenotypes are consistent with PGAP2 related diseases. The two missense variants [c.686C>T (p.Ala229Val) and c.677C>T (p.Thr226Ile)] in PGAP2 gene found in this family were segregation with the disease, while c.677C>T (p.Thr226Ile) was a novel variant. All the two patients showed a positive response to ACTH treatment and high-dose pyridoxine. In summary, this study contributes to expanding the pathogenic variant spectrum of PGAP2 related HPMRS, and provides new insights into the treatment.
Kawasaki disease involving both the nervous system and cardiovascular system: a case report and literature review
Authors: Yin Wen , Wu Yali , Wang Shasha , Tang Hongxia , Ding Yan
Published: December 2024

Background

Kawasaki disease (KD), an acute self-limiting vasculitis, is the main cause of acquired heart disease. Timely diagnosis and treatment can mitigate the occurrence of complications and improve patient prognosis. Facial nerve paralysis (FNP) and ptosis are rare complications of KD and are rarely reported, but FNP is considered a high risk factor for coronary aneurysms. If giant coronary artery aneurysms are formed, clinicians should be vigilant when diagnosing the formation of systemic artery aneurysms (SAAs).

Patient presentation

A 10-month-old girl with fever, diffuse rash, and conjunctival congestion was hospitalized locally, diagnosed with KD, and treated with intravenous infusion of gamma globulin (IVIG). She had fever again after 1 week of temperature stability, accompanied by conjunctival congestion, and was treated with a second dose of IVIG, but she still experienced recurrent fever. The day prior to admission, she developed facial asymmetry, left FNP, diffuse erythema and membranous peeling of the fingers of both hands. The patient's body temperature normalized after treatment with 20 mg/kg methylprednisolone, but cardiac ultrasound revealed progressive enlargement of the coronary artery aneurysms. On day 37of the illness, transient eyelid ptosis developed; fortunately, the left FNP and eyelid ptosis finally resolved, leaving no sequelae. Two years and eight months after onset, the patient developed bilateral humeral aneurysm.

Conclusion

This is the first KD patient involving two neurological complications combined with giant coronary artery aneurysms and SAAs. KD needs to be considered in infants with unexplained recurrent fever who present with FNP or ptosis. FNP secondary to KD is a high risk factor for coronary artery aneurysms, so it is necessary to perform cardiac ultrasound for accurate diagnosis. KDs combined with giant coronary aneurysms require careful physical examination and noninvasive angiography during follow-up to detect SAA formation.
Cardiovascular toxicity risk assessment of tyrosine kinase inhibitors: a pharmacovigilance study using the VigiBase database
Authors: Igawa Yusuke , Hamano Hirofumi , Esumi Satoru , Takeda Tatsuaki , Kajizono Makoto , Kikuoka Ryo , Kimura Ikuya , Zamami Yoshito
Published: December 2024

Introduction

Advances in the early detection and treatment of cancer have significantly improved the prognosis of patients with cancer. Tyrosine kinase inhibitors (TKIs) are effective targeted treatments for various malignancies that act by inhibiting kinase activity. Although these drugs share a common mechanism of action, they differ in their targeted kinases, pharmacokinetics, and side effects. TKIs can cause cardiovascular side effects, which adversely affect the prognosis of cancer survivors. This study aimed to assess the risk of cardiac toxicity associated with TKIs using the World Health Organization Global Database, VigiBase.

Methods

We conducted a cross-sectional analysis of data from VigiBase, a comprehensive global database of suspected drug reactions. The dataset included reports up to December 2022. We identified patients treated with Food and Drug Administration-approved TKIs and analyzed their age and sex data. The primary outcome was cardiovascular impairment, defined by 21 preferred terms in the Medical Dictionary for Regulatory Activities Terminology version 25.1. Disproportionality analysis using the reported odds ratio was performed to detect adverse cardiovascular signals. Statistical analyses were conducted using R 3.3.2, with a P-value <0.05 considered significant.

Results

Of the 32, 520, 983 reports in VigiBase, 23, 181, 539 were eligible for the analysis. Significant cardiovascular signals were identified for 17 TKIs, including erlotinib, gefitinib, and imatinib. Stratified analyses revealed potential sex- and age-related differences in the risk of adverse events. Heatmaps indicated significant signals for drugs such as lapatinib in males and gefitinib in younger patients.

Discussion

Our findings indicate that some TKIs, particularly those classified as VEGFR, BCR-ABL, and BTK, pose similar risks of cardiotoxicity, while others, including EGFR, HER2, and ALK TKIs, exhibit varied risk profiles. These results underscore the importance of individualized risk assessment and management of TKI-treated patients. In conclusion, this study provides valuable insights into the cardiotoxic risk of TKIs, which is essential for developing tailored treatment plans.
Advancements and gaps in natural language processing and machine learning applications in healthcare: a comprehensive review of electronic medical records and medical imaging
Authors: Khalate Priyanka , Gite Shilpa , Pradhan Biswajeet , Lee Chang-Wook
Published: December 2024

This article presents a thorough examination of the progress and limitations in the application of Natural Language Processing (NLP) and Machine Learning (ML), particularly Deep Learning (DL), in the healthcare industry. This paper examines the progress and limitations in the utilisation of Natural Language Processing (NLP) and Machine Learning (ML) in the healthcare field, specifically in relation to Electronic Medical Records (EMRs). The review also examines the incorporation of Natural Language Processing (NLP) and Machine Learning (ML) in medical imaging as a supplementary field, emphasising the transformative impact of these technologies on the analysis of healthcare data and patient care. This review attempts to analyse both fields in order to offer insights into the current state of research and suggest potential chances for future advancements. The focus is on the use of these technologies in Electronic Medical Records (EMRs) and medical imaging. The review methodically detects, chooses, and assesses literature published between 2015 and 2023, utilizing keywords pertaining to natural language processing (NLP) and healthcare in databases such as SCOPUS. After applying precise inclusion criteria, 100 papers were thoroughly examined. The paper emphasizes notable progress in utilizing NLP and ML methodologies to improve healthcare decision-making, extract information from unorganized data, and evaluate medical pictures. The key findings highlight the successful combination of natural language processing (NLP) and image processing to enhance the accuracy of diagnoses and improve patient care. The study also demonstrates the effectiveness of deep learning-based NLP pipelines in extracting valuable information from electronic medical records (EMRs). Additionally, the research suggests that NLP has the potential to optimize the allocation of medical imaging resources. The identified gaps encompass the necessity for scalable and practical implementations, improved interdisciplinary collaboration, the consideration of ethical factors, the analysis of longitudinal patient data, and the customization of approaches for specific medical situations. Subsequent investigations should focus on these deficiencies in order to fully exploit the capabilities of natural language processing (NLP) and machine learning (ML) in the healthcare sector, consequently enhancing patient outcomes and the delivery of healthcare services.
The role of exercise in restoring executive function: a comparison of tobacco-exposed college athletes and sedentary students
Authors: Wang Minjia , Wu Shuya , Ma Qian , Hu Hao , Liu Yanpei , Wang Yaozheng , Zhan Shitao , Liu Dongsen , Girard Olivier
Published: December 2024

Introduction

As adolescent smoking rates rise, its impact on cognitive function has drawn greater attention. This study explores whether exercise can mitigate the negative effects of smoking on executive function in male college students.

Methods

Sixty male college students were divided into four groups (n = 15 each): sedentary smokers, sedentary nonsmokers, athletic smokers, and athletic nonsmokers. All participants completed the Eriksen flanker task, with prefrontal cortex activation measured using functional near-infrared spectroscopy. After the baseline test, all sedentary students engaged in 33 min of high-intensity interval training, followed by the same procedures as in the pre-test.

Results

In the flanker task, college athletes exhibited superior executive function compared to sedentary students, with higher accuracy (p = 0.042), faster reaction times (p = 0.002), and more pronounced brain activation (p = 0.048). Post-exercise, reaction times improved significantly in sedentary groups (p < 0.05). Smoking impaired executive function both before and after exercise, with smokers showing lower accuracy (p < 0.001), slower reaction times (p < 0.001), and diminished brain activation (p < 0.001) compared to nonsmokers.

Discussion

Engaging in acute aerobic exercise may improve executive function in sedentary smokers. Exercise may help mitigate smoking-related declines in executive function among college students.
Genetic control of root/shoot biomass partitioning in barley seedlings
Authors: Cabeza Alejandra , Casas Ana M. , Larruy Beatriz , Costar María Asunción , Martínez Vanesa , Contreras-Moreira Bruno , Igartua Ernesto
Published: December 2024

The process of allocating resources to different plant organs in the early stage of development can affect their adaptation to drought conditions, by influencing water uptake, transpiration, photosynthesis, and carbon storage. Early barley development can affect the response to drought conditions and mitigate yield losses. A distinct behavior of biomass partitioning between two Spanish barley landraces (SBCC073 and SBCC146) was observed in a previous rhizotron experiment. An RIL population of approximately 200 lines, derived from the cross of those lines, was advanced using speed breeding. We devised an experiment to test if seedling biomass partitioning was under genetic control, growing the seedlings in pots filled with silica sand, in a growth chamber under controlled conditions. After 1 week, the shoot and root were separated, oven dried, and weighted. There were genotypic differences for shoot dry weight, root dry weight, and root-to-shoot ratio. The population was genotyped with a commercial 15k SNP chip, and a genetic map was constructed with 1,353 SNP markers. A QTL analysis revealed no QTL for shoot or root dry weight. However, a clear single QTL for biomass partitioning (RatioRS) was found, in the long arm of chromosome 5H. By exploring the high-confidence genes in the region surrounding the QTL peak, five genes with missense mutations between SBCC146 and SBCC073, and differential expression in roots compared to other organs, were identified. We provide evidence of five promising candidate genes with a role in biomass partitioning that deserve further research.
Health-related quality of life in children born preterm at school age: the mediating role of social support and maternal stress
Authors: Martínez-Shaw Melissa Liher , Evensen Kari Anne I. , Melero Sandra , Sánchez-Sandoval Yolanda
Published: December 2024

Research on health-related quality of life (HRQoL) of school-aged children born preterm (< 37 weeks of gestational age) is scarce and there are few studies examining the relationship with medical and family factors. The aims were to analyze HRQoL in a sample of 8-year-old children born preterm with very low birth weight (VLBW), to test a proposed theoretical model that examines the relationship with medical and socio-family factors, and to explore the mediation effects of maternal factors between perinatal variables, demographic characteristics and HRQoL. A total of 147 VLBW children and 116 mothers were assessed. The measures included for assessment were self-and parent-reported HRQoL, functional social support, maternal stress, socio-family risk index and neonatal medical risk index. Mediation analysis was applied to investigate mediation effects of the maternal factors. Mean self-and parent-reported KIDSCREEN scores were 55.1 (SD 10.1) and 58.2 (SD 9.1), respectively, indicating better HRQoL than the normed sample with a mean of 50 (p < 0.001). The total effect of the initial theoretical model was not significant, thus another partial model was validated. Socio-family risk index significantly influenced HRQoL (direct effect), and this relationship was mediated by functional social support and maternal stress (indirect effects). School-aged VLBW children and their parents reported better HRQoL than the mean reference value on KIDSCREEN-10 and -27. Maternal stress and social support had a mediating effect on the children’s HRQoL. These results could be used to tailor interventions in these families.
Analysis of the health effects of multiple social networks on the older adult: the substitution role of labor participation
Authors: Li Zhiying
Published: December 2024

Objectives

This study aims to examine the role of social networks in influencing the physical and mental health of older Chinese adults, investigating both the underlying mechanisms and the associations between social networks, labor force participation, and health outcomes.

Methods

Using data from the 2021 China General Social Survey (CGSS), we analyzed a sample of 1,332 older adults, incorporating demographic and health-related variables. Ordinary least squares (OLS) regression and mediation analysis were conducted to evaluate the effects of social networks on health outcomes, with subgroup analyses by gender and education level. Stata 18.0 and SPSS were employed to perform all statistical analyses.

Results

Social networks demonstrate a significant positive effect on overall, physical, and mental health at the 1% level, with weak-tie networks exhibiting a more substantial impact on health (β = 0.1146, p < 0.01). In promoting physical health, social networks have a coefficient of 0.1371 (p < 0.001) for females and 0.2128 (p < 0.001) for males. Among individuals with lower education, the coefficient is 0.1561 (p < 0.001), while for those with higher education, it is 0.2184 (p < 0.001). Regarding mental health, social networks yield a coefficient of 0.0747 (p > 0.05) for females and 0.1095 (p < 0.01) for males; for individuals with lower education, the coefficient is 0.0914 (p < 0.01), and for those with higher education, it is 0.0441 (p > 0.05). Media use, subjective wellbeing, and perceived social class are key explanatory mechanisms in the relationship between social networks and health of the older adult. Notably, subjective wellbeing and perceived social class function as chain mediators between social networks and health outcomes. The interaction between social networks and labor participation reveals a significant negative coefficient (β = −0.1864, p < 0.01).

Conclusion

Social networks contribute to improved health in older adults, with weak ties playing a particularly significant role, although the effect varies across subgroups. Media use, subjective social class, and wellbeing are important mechanisms linking social networks with older adult health, while labor force participation may serve as a substitute for social networks in health promotion among older adults. This study will inform the improvement of older adults health and the development of labor policies for older adults.
Rare cell-based liquid biopsy for improved measurement of low-grade chronic inflammation
Authors: Schreier Stefan , Budchart Prapaphan , Borwornpinyo Suparerk , Lertsithichai Panuwat , Triampo Wannapong
Published: December 2024

Objectives and design

Low-grade inflammation is a hallmark of chronic diseases. More sensitive tools for chronic low-grade inflammation detection are needed and herein presented as a proof of concept. Heightened sensitivity to inflammation may be achieved by analyzing the compensation mechanisms of hematopoiesis in response to stress. The production of red blood cells and platelets, which are particularly vulnerable to physiological imbalances, are especially important in this context due to their high turnover rates. The compensatory mechanisms involve the production or release of rare immature blood cell types that herein serve as important biomarker targets.

Methods

A cell-based liquid biopsy platform, using negative selection, was used to detect circulating rare cells in comprehension, allowing simultaneous analysis of an immature cell panel from one sample. The concentration ranges under physiological conditions for each cell marker were evaluated on a self-reported healthy control cohort and prospectively tested on three individuals undergoing various interventions: one afflicted with early-stage breast cancer, another with atherosclerosis in follow-up, and a third healthy individual with cardiovascular disease risk.

Results

The approach effectively identified rare cellular abnormalities in asymptomatic individuals who exhibited no abnormalities in their complete blood counts. This condition was designated as silent inflammation (SI). SI was effective in monitoring response to intervention and predicting inflammation state.

Conclusions

The detection of SI proved valuable in aiding inflammation differential diagnosis and for monitoring the response to interventions in all three subjects.
Testosterone/cortisol ratio: gender effect and prediction of podium results in beach sprint master rowers
Authors: Ficarra Giovanni , Rottura Michelangelo , Mannucci Carmen , Caccamo Daniela , Bitto Alessandra , Trimarchi Fabio , Di Mauro Debora
Published: December 2024

Objective

The purpose of this study was to address the lack of data on the stress and motivation response in master athletes during competitions, as athletic performance in this age group can be significantly impacted by stress rather than appropriate training.

Methods

Coastal rowing beach sprint master athletes aged 43–57 years were examined to assess the saliva levels of stress biomarkers, cortisol, and testosterone. Specifically, samples were collected at awakening and before and after the boat race.

Results

Mean values of cortisol remained unchanged from awakening and raised at the end of the competition, while testosterone levels increased before the race, suggesting an aggressive/competitive behavior. Cortisol levels were significantly higher when comparing pre-race levels with post-race (p = 0.001) levels and early morning with post-race (p = 0.006) levels. No gender effect was observed in this case. Testosterone values did not demonstrate significance when compared between time points, not even when stratifying by gender. Considering the 24 athletes, a higher testosterone/cortisol ratio was correlated with a worse podium position (B = 3.705; p = 0.009). When stratified by gender, the testosterone/cortisol ratio demonstrated an association with a worse outcome of the race only in female rowers (B = 4.012; p = 0.022). Male athletes demonstrated no significant correlation between hormone ratio and race results (B = 3.288; p = 0.292).

Conclusion

It emerged from this study that the amateur rowers who approach competitive sport during adulthood may have problems in coping with the race-related stress and thus the outcome of their performance might be affected, as in adolescents.
Intensifying homestead climate-smart agriculture and the challenges to its wider adoption in Azuari watershed, Northwest Ethiopia
Authors: Debie Ermias
Published: December 2024

Introduction

The adoption of climate-smart agriculture practices (CSAPs) at the plot level is a promising yet underutilized strategy in Ethiopia, where subsistence farming predominates. This study investigates the factors influencing the intensity of CSAPs on homesteads and identifies key barriers to their broader adoption.

Methods

Quantitative data were collected through household surveys. Multiple Linear Regression (MLR) analysis was employed to examine the relationship between the independent variables and farmers' decisions to intensify CSAPs.

Results

The findings reveal that slope gradient, extension contacts, farming experience, dependency ratio-induced labor, and livestock diversity significantly impact farmers' decisions to enhance CSAPs. Major barriers include insecure land tenure, poor management of open grazing, labor-intensive practices, and limited access to agricultural inputs.

Discussion

To promote the widespread implementation of CSAPs, it is imperative that these variables are prioritized in agricultural extension policies. Addressing these barriers is essential for enhancing the adoption of CSAPs, which will significantly contribute to sustainable agricultural development in Ethiopia.
Prognostic and clinicopathological significance of survivin in gynecological cancer
Authors: Chuwa Agapiti H. , Mvunta David H.
Published: December 2024

Survivin belongs to the inhibitor of apoptosis protein (IAP) family and is encoded by the baculoviral inhibitor of apoptosis repeat-containing, or BIRC5, gene. It is preferentially expressed in cancers with functional complexity in cell signaling cascades such as extracellular signal-regulated kinases (ERK), mitogen-activated protein kinases (MAPK), heat shock protein-90 (HSP90), epidermal growth factor receptor (EGFR), phosphoinositide 3-kinase (PI3K), signal transducer and activator of transcription (STAT), hypoxia-inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF), and others. Survivin plays a role in cell division and cell death, properties that have attracted a large body of research to decipher its therapeutic and prognostic significance in cancer. Survivin has tumor-promoting effects in endometrial (EC) and ovarian (OC) cancers, and its upregulation in endometrial cancer has been associated with poor overall survival (OS). While survivin protein is abundantly expressed in OC, it is barely detectable in normal ovarian tissue or benign ovarian tumors. Survivin expression is also a marker for cervical intraepithelial neoplasia (CIN) and high-risk human papillomavirus, and a predictor of viral clearance and prognosis in uterine cervical cancer (UCC). Furthermore, nuclear survivin expression is very low in normal vulvar squamous epithelium and increases to become abundant in vulvar invasive squamous cell carcinoma (ISCC), conferring resistance to apoptosis in vulvar carcinogenesis. In this review, we discuss in detail the impact of survivin signaling on gynecological cancers and provide insight on its therapeutic and diagnostic potential, existing research gaps, and areas for future research.
Combination of farnesyl-transferase inhibition with KRAS G12D targeting breaks down therapeutic resistance in pancreatic cancer
Authors: Molnár Eszter , Baranyi Marcell , Szigeti Krisztina , Hegedűs Luca , Bordás Fanni , Gábriel Zsófia , Petényi Gréta , Tóvári József , Hegedűs Balázs , Tímár József
Published: December 2024

Pancreatic adenocarcinoma is one of the deadliest forms of cancer with no effective therapeutic options. A KRAS mutation can be found in up to 90% of all pancreatic tumors, making it a promising therapeutic target. The introduction of new KRAS inhibitors has been a milestone in the history of KRAS mutant tumors; however, therapeutic resistance limits their efficacy. Thus, new therapeutic options, including combination therapies, are urgently needed. Recently, we have shown that KRAS G12C inhibitors in combination with farnesyl-transferase inhibitors exert synergistic antitumor effects. Here, we provide evidence for the feasibility of this combinational approach to break down resistance in KRAS G12D mutant pancreatic cancer. Although we have shown that the 3D environment dramatically sensitizes cells to MRTX1133 treatment, the synergistic effect of this drug combination is present in both 2D and 3D in the PANC1 pancreatic adenocarcinoma model, which showed high resistance to MRTX1133 in 2D. The effects of the combination treatment show an association with the inhibition of farnesylated regulatory proteins, including HRAS and RHEB, along with the expression level of KRAS. Our study warrants further investigation for the potential applicability of KRAS G12D inhibitors in combination with farnesyl-transferase inhibitors for the treatment of KRAS mutant pancreatic adenocarcinoma.
Optimizing Sorghum for California: A Multi-Location Evaluation of Biomass Yield, Feed Quality, and Biofuel Feedstock Potential
Authors: Atim Jackie , Kaweesi Tadeo , Hutmacher Robert B. , Putnam Daniel H. , Pedraza Julie , de Ben Christopher M. , Schramm Tarilee , Angeles Jorge , Clark Nicholas E. , Dahlberg Jeffery A.
Published: December 2024

Sorghum cultivars, particularly those used for forage and biomass, present significant potential as drought-resistant crops suitable for animal feed and biofuel production. This study evaluated 59 sorghum hybrids over five years (2019–2023) across three University of California research farm locations in the Central Valley: Kearney REC (KARE), West Side REC (WSREC), and Davis. The primary aim was to identify genotypes that exhibit high yield and stability across diverse environments in California, which is crucial for meeting the state’s significant feed needs associated with dairy operations and animal production. The evaluation focused on biomass yields, forage quality traits such as Relative Feed Quality (RFQ) and milk yield per ton (milk/ton), and biofuel-relevant chemical compositions like Neutral Detergent Fiber (NDF) and starch. A multi-trait stability index was employed to pinpoint superior genotypes that combine high yield with desirable quality traits. Results indicated significant genotypic, environmental, and genotype-by-environment (GxE) interaction effects for all traits except fat and water-soluble sugars. Eight hybrids were notable for maintaining high and stable biomass yields across different locations. Additionally, high fat and starch content were found to correlate with improved milk/ton potential, while lower fiber content (ADF, NDF) was associated with enhanced RFQ. Specifically, nine hybrids were identified as optimal for dairy forage due to their combination of high yield, RFQ, and milk/ton. Furthermore, distinct hybrids were identified for first-generation (starch-based) and second-generation (NDF-based) biofuel strategies. Three hybrids stood out as having desirable traits for both feed and biofuel applications, underscoring their versatility. This study highlights the utility of a multi-trait stability index in selecting superior sorghum genotypes for specific trait combinations. The identified candidates for forage and biofuel use, especially the multipurpose varieties, offer valuable insights that can aid growers and industry stakeholders in developing more sustainable and versatile sorghum production systems in California. Findings from this study contribute significantly to the development of more resilient sorghum production systems. By identifying hybrids that excel in both yield and quality across various environments, this research supports future cropping decisions aimed at enhancing water use efficiency and drought resilience in sorghum cultivation. These advancements are crucial for maintaining competitive dairy operations and advancing biofuel production in the face of climate change-induced challenges.
Introducing Ferrous Sulfate to Cattle Manure and Corn Straw Composting Reduces Greenhouse Gas Emissions and Ammonia Volatilization
Authors: Geng Yucong , Bashir Muhammad Amjad , Wang Hongyuan , Li Jungai , Raza Qurat-Ul-Ain , Kan Weijie , Tian Shuo , Rehim Abdur , Yang Longcheng , Liu Hongbin
Published: December 2024

Composting is a well-known method for waste management, but it causes greenhouse gas emissions. Various techniques have been used to reduce emissions and improve the quality of compost, but they resulted in an increased composting time. Keeping in view the above points, the current study aimed to reduce the composting time and gas emissions along with improving the nutritional value of compost using FeSO4 as an additive to cattle manure and corn straw composting. Seven treatments were established, including control (CK) without FeSO4 and six levels of FeSO4 (0.25%, 0.50%, 1%, 2%, 4%, 8%). The results revealed that FeSO4 reduced the CH4 (36.1–36.7%), H2S (10.7–34.5%), N2O (17.2–48.5%), and NH3 (18.3–69.0%) emissions compared to CK. In addition, the total N (8.4–40.0%) content in compost products was also improved. The study showed that a higher dose of applied FeSO4 can significantly reduce emissions, but it reduces the temperature at the start of composting resulting in an increase in the composting time, while the lower dose (0.5–1%) also has the capability to reduce the emissions compared with the control without negatively affecting the temperature rise. The study concludes that using 0.5–1% of FeSO4 can effectively utilize its inhibitory action of decomposition that mitigates gas emissions and prepares an N-enriched compost.
Effect of Longer Pre-Starter Diet Allowance on Post-Weaning Performance of Lightweight Piglets
Authors: González-Solé Francesc , Solà-Oriol David , Villagómez Estrada Sandra , Muns Ramon , Pérez José Francisco
Published: December 2024

First-phase diets are formulated to help pigs reach a target body weight before switching to a more affordable diet. However, these transitions typically take place on a fixed schedule, which may disadvantage low-body-weight piglets due to their underdeveloped digestive systems and limited feed intake capacity. This study examined the effects of offering low-body-weight (BW) pigs a pre-starter diet until they reached a target BW on growth performance and BW variability. A total of 528 pigs ([Landrace × Yorkshire] × Pietrain), weaned at day 21, representing the smallest 50% of their batch, were divided into medium (5.35 ± 0.31 kg) and small (3.97 ± 0.21 kg) groups based on weaning BW. Pigs received a pre-starter diet either for 10 days (FIXED) or until they reached a target BW of 7.9 kg (TBW), followed by a starter feed. Growth performance between the TBW and fixed strategies was similar over the 36-day period. Overall, mortality was higher in small pigs, though small pigs fed using the fixed strategy showed higher mortality than the rest of the groups between days 10 and 36. This study concludes that offering a pre-starter diet based on target BW, instead of a fixed time, did not significantly improve growth performance or batch uniformity but reduced mortality in small pigs from days 10 to 36.
Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
Authors: Zhang Yu , Zhao Xuefei , Li Shuqi , Xu Yanchun , Bai Suying , Zhang Wei
Published: December 2024

The hair, an exclusive keratinized dermal appendage in mammals, stands as a quintessential outcome of adaptive evolution, conferring resilience against adverse environmental conditions. The ontogenesis of the coat displays a pronounced rhythmic pattern, with hair follicle stem cells (HFSCs) emerging as pivotal facilitators of hair follicle reconstitution. The retinoic acid-related orphan receptor alpha, a nuclear receptor with extensive involvement in the regulation of cellular physiological states, exerts its functions predominantly through the modulation of downstream target gene transcription. The Bnip3 gene exhibits a robust correlation with cellular apoptosis and autophagy, which are indispensable physiological mechanisms underlying the maintenance of HFSC homeostasis. Consequently, the expression level of Bnip3 may be intimately linked to the status of HFSCs. In this investigative endeavor, we employed rat HFSCs as a model system to validate the regulatory impact of RORA on Bnip3 gene expression. Our findings unequivocally demonstrate that Bnip3 serves as a direct downstream target of RORA. Specifically, RORA binds to the motif within the Bnip3 promoter region, thereby upregulating Bnip3 expression levels. In light of our research findings, we propose that RORA holds potential as a target for modulating the status of HFSCs.
Cardiac Autonomic Modulation in Domestic Cats with Obstructive Lower Urinary Tract Disease
Authors: Tarazona Molina Mary Marlene , Guimarães-Okamoto Priscylla Tatiana Chalfun , Cruz-Aleixo Amanda Sarita , Tsunemi Miriam Harumi , Valença Corrêa Jaqueline , Cardia de Melo Jessica , Machado Luiz Henrique de Araújo , Lourenço Maria Lucia Gomes
Published: December 2024

Obstructive feline lower urinary tract disease (OFLUTD) is the most common complication of the urinary system, as metabolic and electrolyte changes can alter the functioning of the autonomic nervous system (ANS). The objective of this study was to describe the indices of heart rate variability (HRV) and their correlations with the observed alterations in Systolic Blood Pressure (SBP) and electrocardiographic, biochemical, and haemogasometric indices in cats diagnosed with OFLUTD. Sixty-five male cats up to 10 years of age were divided into two groups. The control group (CG) was composed of 25 healthy cats, and the obstructed group (OG) consisted of 40 cats with OFLUTD. The OG was evaluated by electrocardiography and blood pressure at four different time points. In the CG, electrocardiographic evaluation and SBP measurement were performed. A comparison of the HRV between the CG and OG (M0) revealed differences in the SDNN (standard deviation of all normal RR-NN) parameters (sympathetic and parasympathetic tone) and in the rMSSD (parasympathetic tone); there was a difference in the SBP, which was greater in the CG. There were higher rates in the CG. The HF and HR were greater in OG. The HRV serves as a preventive tool and predicts the severity of OFLUTD in patients due to an imbalance in the ANS.
Prevention and Modern Strategies for Managing Methicillin-Resistant Staphylococcal Infections in Prosthetic Joint Infections (PJIs)
Authors: Kraus Karolina , Mikziński Paweł , Widelski Jarosław , Paluch Emil
Published: December 2024

Periprosthetic joint infections (PJIs) are a dangerous complication of joint replacement surgeries which have become much more common in recent years (mostly hip and knee replacement surgeries). Such a condition can lead to many health issues and often requires reoperation. Staphylococci is a bacterial group most common in terms of the pathogens causing PJIs. S. aureus and coagulase-negative staphylococci are found in around two-thirds of PJI cases. Recently, the numbers of staphylococci that cause such infections and that are methicillin-resistant are increasing. This trend leads to difficulties in the treatment and prevention of such infections. That is why MRSA and MRSE groups require extraordinary attention when dealing with PJIs in order to successfully treat them. Controlling carriage, using optimal prosthetic materials, and implementing perioperative antimicrobial prophylaxis are crucial strategies in infection prevention and are as essential as quick diagnosis and effective targeted treatment. The comprehensive professional procedures presented in this review show how to deal with such cases.
Novel Variant of New Delhi Metallo-Beta-Lactamase (blaNDM-60) Discovered in a Clinical Strain of Escherichia coli from the United Arab Emirates: An Emerging Challenge in Antimicrobial Resistance
Authors: Al-Marzooq Farah , Ghazawi Akela , Allam Mushal , Collyns Timothy , Saleem Aqeel
Published: December 2024

Background/Objectives: Carbapenem resistance poses a significant health threat. This study reports the first detection and characterization of a novel variant of New Delhi metallo-β-lactamase (blaNDM-60) in Escherichia coli from the United Arab Emirates (UAE), including its genetic context and relationship to global strains. Methods: NDM-60-producing E. coli was isolated from a rectal swab during routine screening. Characterization involved whole-genome sequencing, antimicrobial susceptibility testing, and comparative genomic analysis with 66 known NDM variants. Core genome analysis was performed against 42 global E. coli strains, including the single other reported NDM-60-positive isolate. Results: The strain demonstrated extensive drug resistance, including resistance to novel β-lactam/β-lactamase inhibitor combinations, notably taniborbactam. NDM-60 differs from the closely related NDM-5 by a single amino acid substitution (Asp202Asn) and two amino acid substitutions (Val88Leu and Met154Leu) compared to NDM-1. NDM-60 is located on a nonconjugative IncX3 plasmid. The strain belongs to sequence type 940 (ST940). Phylogenetic analysis revealed high diversity among the global ST940 strains, which carry a plethora of resistance genes and originated from humans, animals, and the environment from diverse geographic locations. Conclusions: NDM-60 emergence in the UAE represents a significant evolution in carbapenemase diversity. Its presence on a nonconjugative plasmid may limit spread; however, its extensive resistance profile is concerning. Further studies are needed to determine the prevalence, dissemination, and clinical impact of NDM-60. NDM evolution underscores the ongoing challenge in managing antimicrobial resistance and the critical importance of vigilant molecular surveillance. It also highlights the pressing demand to discover new antibiotics to fight resistant bacteria.