bluuebunny/crossref_metadata_2025 huggingface dataset:
Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis
Authors: Cirkovic Andja , Stanisavljevic Dejana , Milin-Lazovic Jelena , Rajovic Nina , Pavlovic Vedrana , Milicevic Ognjen , Savic Marko , Kostic Peric Jelena , Aleksic Natasa , Milic Nikola , Stanisavljevic Tamara , Mikovic Zeljko , Garovic Vesna , Milic Natasa
Published: December 2021

Introduction: Preeclampsia (PE) is a pregnancy-associated, multi-organ, life-threatening disease that appears after the 20th week of gestation. The aim of this study was to perform a systematic review and meta-analysis to determine whether women with PE have disrupted miRNA expression compared to women who do not have PE.

Methods: We conducted a systematic review and meta-analysis of studies that reported miRNAs expression levels in placenta or peripheral blood of pregnant women with vs. without PE. Studies published before October 29, 2021 were identified through PubMed, EMBASE and Web of Science. Two reviewers used predefined forms and protocols to evaluate independently the eligibility of studies based on titles and abstracts and to perform full-text screening, data abstraction and quality assessment. Standardized mean difference (SMD) was used as a measure of effect size.

Results: 229 publications were included in the systematic review and 53 in the meta-analysis. The expression levels in placenta were significantly higher in women with PE compared to women without PE for miRNA-16 (SMD = 1.51,95%CI = 0.55–2.46), miRNA-20b (SMD = 0.89, 95%CI = 0.33–1.45), miRNA-23a (SMD = 2.02, 95%CI = 1.25–2.78), miRNA-29b (SMD = 1.37, 95%CI = 0.36–2.37), miRNA-155 (SMD = 2.99, 95%CI = 0.83–5.14) and miRNA-210 (SMD = 1.63, 95%CI = 0.69–2.58), and significantly lower for miRNA-376c (SMD = –4.86, 95%CI = –9.51 to –0.20). An increased level of miRNK-155 expression was found in peripheral blood of women with PE (SMD = 2.06, 95%CI = 0.35–3.76), while the expression level of miRNA-16 was significantly lower in peripheral blood of PE women (SMD = –0.47, 95%CI = –0.91 to –0.03). The functional roles of the presented miRNAs include control of trophoblast proliferation, migration, invasion, apoptosis, differentiation, cellular metabolism and angiogenesis.

Conclusion: miRNAs play an important role in the pathophysiology of PE. The identification of differentially expressed miRNAs in maternal blood creates an opportunity to define an easily accessible biomarker of PE.
Hybrid Hydrogel Composed of Hyaluronic Acid, Gelatin, and Extracellular Cartilage Matrix for Perforated TM Repair
Authors: Wang Yili , Wen Feng , Yao Xueting , Zeng Lulu , Wu Jiaming , He Qinhong , Li Huaqiong , Fang Lian
Published: December 2021

A novel series of composite hydrogels, built from the three components 1), hyaluronic acid methacryloyl (HAMA); 2), gelatin methacryloyl (GelMA), and 3), extracellular cartilage matrix (ECM), was prepared and studied regarding the possible utility in the surgical repair of damaged (perforated) tympanic membrane (TM). Noteworthy is component 3), which was harvested from the ribs of α-1,3-galactosidyltransferase-knockout (α-1,3 GalT-KO) pigs. The absence of α-1,3-galactosyl glycoprotein is hypothesized to prevent rejection due to foreign-body immunogenicity. The composite hydrogels were characterized by various aspects, using a variety of physicochemical techniques: aqueous swelling, structural degradation, behavior under compression, and morphology, e.g., in vitro biocompatibility was assessed by the CCK-8 and live–dead assays and through cytoskeleton staining/microscopy. Alcian blue staining and real-time PCR (RT-PCR) were performed to examine the chondrogenic induction potential of the hydrogels. Moreover, a rat TM defect model was used to evaluate the in vivo performance of the hydrogels in this particular application. Taken together, the results from this study are surprising and promising. Much further development work will be required to make the material ready for surgical use.
Environmental Health Perceptions in University Classrooms: Results From an Online Survey During the COVID-19 Pandemic in the United States and Colombia
Authors: Coronado Maria Camila , Rockcastle Siobhan , Kwok Alison
Published: December 2021

At the start of the COVID-19 pandemic, multiple institutions mobilized to create recommendations that maximize indoor environmental quality to keep building occupants as safe as possible. Many of those recommendations were implemented in buildings across the world even before the presence of field data validated their effectiveness. However, as the pandemic raised awareness of the risks present in the air quality of everyday built environments, little is known about how occupants perceive these interventions or how they will perceive buildings when activities resume with an in-person requirement. This paper presents results from an online survey, conducted from April 23rd to May 8th, 2021, and offers a window into the perceptions of university students on a variety of physical intervention strategies that have been promoted for the return to classrooms. Our analysis explores differences in these perceptions between students from Colombia and the United States, where the vaccination rates of the population as of May 2021 differed substantially. An anonymous online survey was disseminated to students of higher academic institutions using images portraying 3D models of classrooms and written prompts to assess perceptions. Health risk and health promotion perceptions were evaluated using four categories: visual connection to the outdoors, occupant density, additional furnishings, ventilation system type, and number of operable windows. While the effectiveness of these strategies from an epidemiological standpoint was not considered, this survey aimed to measure the perceived effectiveness of these strategies as students prepare to return to in-person education in the classroom. Our analysis found a significant effect of different degrees of “connection to the outdoors” and “occupant density” on both perceived health risk and health promotion in both countries. Respondents ranked strategies like mask-wearing and natural ventilation as important interventions when considering a return to the classroom. Perceptions of health risk and health promotion between students taking classes in the United States and Colombia were significantly different regarding the presence of additional furnishings, and ventilation systems and number of windows in a classroom. The results presented here may shed light on how aspects of the built environment help shape perceptions of healthy environments during the pandemic.
DNA Methylation Data-Based Classification and Identification of Prognostic Signature of Children With Wilms Tumor
Authors: Tang Fucai , Lu Zeguang , Lei Hanqi , Lai Yongchang , Lu Zechao , Li Zhibiao , Tang Zhicheng , Zhang Jiahao , He Zhaohui
Published: December 2021

Background: As an epigenetic alteration, DNA methylation plays an important role in early Wilms tumorigenesis and is possibly used as marker to improve the diagnosis and classification of tumor heterogeneity.

Methods: Methylation data, RNA-sequencing (RNA-seq) data, and corresponding clinical information were downloaded from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. The prognostic values of DNA methylation subtypes in Wilms tumor were identified.

Results: Four prognostic subtypes of Wilms tumor patients were identified by consensus cluster analysis performed on 312 independent prognostic CpG sites. Cluster one showed the best prognosis, whereas Cluster two represented the worst prognosis. Unique CpG sites identified in Cluster one that were not identified in other subtypes were assessed to construct a prognostic signature. The prognostic methylation risk score was closely related to prognosis, and the area under the curve (AUC) was 0.802. Furthermore, the risk score based on prognostic signature was identified as an independent prognostic factor for Wilms tumor in univariate and multivariate Cox regression analyses. Finally, the abundance of B cell infiltration was higher in the low-risk group than in the high-risk group, based on the methylation data.

Conclusion: Collectively, we divided Wilms tumor cases into four prognostic subtypes, which could efficiently identify high-risk Wilms tumor patients. Prognostic methylation risk scores that were significantly associated with the adverse clinical outcomes were established, and this prognostic signature was able to predict the prognosis of Wilms tumor in children, which may be useful in guiding clinicians in therapeutic decision-making. Further independent studies are needed to validate and advance this hypothesis.
Transcriptomic and Metabolomic Profiling in Helicobacter pylori–Induced Gastric Cancer Identified Prognosis- and Immunotherapy-Relevant Gene Signatures
Authors: Liu Duanrui , Zhu Jingyu , Ma Xiaoli , Zhang Lulu , Wu Yufei , Zhu Wenshuai , Xing Yuanxin , Jia Yanfei , Wang Yunshan
Published: December 2021

Background: Chronic Helicobacter pylori (HP) infection is considered the major cause of non-cardia gastric cancer (GC). However, how HP infection influences the metabolism and further regulates the progression of GC remains unknown.

Methods: We comprehensively evaluated the metabolic pattern of HP-positive (HP+) GC samples using transcriptomic data and correlated these patterns with tumor microenvironment (TME)–infiltrating characteristics. The metabolic score was constructed to quantify metabolic patterns of individual tumors using principal component analysis (PCA) algorithms. The expression alterations of key metabolism-related genes (MRGs) and downstream metabolites were validated by PCR and untargeted metabolomics analysis.

Results: Two distinct metabolic patterns and differential metabolic scores were identified in HP+ GC, which had various biological pathways in common and were associated with clinical outcomes. TME-infiltrating profiles under both patterns were highly consistent with the immunophenotype. Furthermore, the analysis indicated that a low metabolic score was correlated with an increased EMT subtype, immunosuppression status, and worse survival. Importantly, we identified that the expression of five MRGs, GSS, GMPPA, OGDH, SGPP2, and PIK3CA, was remarkably correlated with HP infection, patient survival, and therapy response. Furthermore, the carbohydrate metabolism and citric acid may be downstream regulators of the function of metabolic genes in HP-induced GC.

Conclusion: Our findings suggest that there is cross talk between metabolism and immune promotion during HP infection. MRG-specific transcriptional alterations may serve as predictive biomarkers of survival outcomes and potential targets for treatment of patients with HP-induced GC.
Ion-Doped Photonic Crystal Fiber Lasers
Authors: Hou Ya-Chong , Li Yun-Fei , Xie Xiao-Fan , Kou Zi-Long , Lu Yue , Chen Si-Ying , Wang Yulei , Lu Zhiwei
Published: December 2021

Compared with conventional solid-state lasers, fiber lasers have the advantages of small size, simple cooling system, and good output beam quality, enabling them an extended service lifetime in industrialized environments. Periodically arranged photonic crystals have been the most important gain medium for high-power laser applications, which overcame the problems in fiber lasers such as small mode field, low degree of nonlinearity, and non-adjustable dispersion. In this mini-review, we summarize the recent advances of typical ion-doped photonic crystal fiber lasers doped, discuss the challenges, and provide an outlook on the future developments in ion-doped photonic crystal fiber lasers.
Metal-Free Pyrene-Based Conjugated Microporous Polymer Catalyst Bearing N- and S-Sites for Photoelectrochemical Oxygen Evolution Reaction
Authors: Das Sabuj Kanti , Shyamal Sanjib , Das Manisha , Mondal Saptarsi , Chowdhury Avik , Chakraborty Debabrata , Dey Ramendra Sundar , Bhaumik Asim
Published: December 2021

The development of an efficient, sustainable, and inexpensive metal-free catalyst for oxygen evolution reaction (OER) via photoelectrochemical water splitting is very demanding for energy conversion processes such as green fuel generators, fuel cells, and metal-air batteries. Herein, we have developed a metal-free pyrene-based nitrogen and sulfur containing conjugated microporous polymer having a high Brunauer-Emmett-Teller surface area (761 m2 g−1) and a low bandgap of 2.09 eV for oxygen evolution reaction (OER) in alkaline solution. The π-conjugated as-synthesized porous organic material (PBTDZ) has been characterized by Fourier transform infrared spectroscopy (FT-IR), solid-state 13C (cross-polarization magic angle spinning-nuclear magnetic resonance) CP-MAS NMR, N2 adsorption/desorption analysis, field-emission scanning electron microscope (FESEM), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA) experiments. The material acts as an efficient catalyst for photoelectrochemical OER with a current density of 80 mA/cm2 at 0.8 V vs. Ag/AgCl and delivered 104 µmol of oxygen in a 2 h run. The presence of low bandgap energy, π-conjugated conducting polymeric skeleton bearing donor heteroatoms (N and S), and higher specific surface area associated with inherent microporosity are responsible for this admirable photoelectrocatalytic activity of PBTDZ catalyst.
Multipurpose Drugs Active Against Both Plasmodium spp. and Microorganisms: Potential Application for New Drug Development
Authors: Endo Takuro , Takemae Hitoshi , Sharma Indu , Furuya Tetsuya
Published: December 2021

Malaria, a disease caused by the protozoan parasites Plasmodium spp., is still causing serious problems in endemic regions in the world. Although the WHO recommends artemisinin combination therapies for the treatment of malaria patients, the emergence of artemisinin-resistant parasites has become a serious issue and underscores the need for the development of new antimalarial drugs. On the other hand, new and re-emergences of infectious diseases, such as the influenza pandemic, Ebola virus disease, and COVID-19, are urging the world to develop effective chemotherapeutic agents against the causative viruses, which are not achieved to the desired level yet. In this review article, we describe existing drugs which are active against both Plasmodium spp. and microorganisms including viruses, bacteria, and fungi. We also focus on the current knowledge about the mechanism of actions of these drugs. Our major aims of this article are to describe examples of drugs that kill both Plasmodium parasites and other microbes and to provide valuable information to help find new ideas for developing novel drugs, rather than merely augmenting already existing drug repurposing efforts.
Comparison of Resting Full-Cycle Ratio and Fractional Flow Reserve in a German Real-World Cohort
Authors: Wienemann Hendrik , Meyer Annika , Mauri Victor , Baar Till , Adam Matti , Baldus Stephan , Halbach Marcel
Published: December 2021

Objective: The aim of this study was to evaluate non-hyperemic resting pressure ratios (NHPRs), especially the novel “resting full-cycle ratio” (RFR; lowest pressure distal to the stenosis/aortic pressure during the entire cardiac cycle), compared to the gold standard fractional flow reserve (FFR) in a “real-world” setting.

Methods: The study included patients undergoing coronary pressure wire studies at one German University Hospital. No patients were excluded based on any baseline or procedural characteristics, except for insufficient quality of traces. The diagnostic performance of four NHPRs vs. FFR ≤ 0.80 was tested. Morphological characteristics of stenoses were analyzed by quantitative coronary angiography.

Results: 617 patients with 712 coronary lesions were included. RFR showed a significant correlation with FFR (r = 0.766, p < 0.01). Diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of RFR were 78% (95% confidence interval = 75; 81), 72% (65; 78), 81% (77; 84), 63% (57; 69), and 86% (83; 89). Relevant predictors for discordance of RFR ≤ 0.89/FFR > 0.8 were LAD lesions, peripheral artery disease, age, female sex and non-focal stenoses. Predictors for discordance of RFR > 0.89/FFR ≤ 0.8 included non-LCX lesions, percent diameter stenosis and previous percutaneous coronary intervention in the target vessel. RFR and all other NHPRs were highly correlated with each other.

Conclusion: All NHPRs have a similar correlation with the gold standard FFR and may facilitate the acceptance and implementation of physiological assessments of lesion severity. However, we found ~20% discordant results between NHPRs and FFR in our “all-comers” German cohort.
Tailings Dam Break: The Influence of Slurry with Different Concentrations Downstream
Authors: Chen Xuanyi , Jing Xiaofei , Chen Yulong , Pan Changshu , Wang Wensong
Published: December 2021

The risk of tailings dam-break disaster is dependent on the type of slurry and its flow characteristics. The flow characteristics of slurry surging from tailings dams collapse are directly influenced by grain size, breach width, slurry concentration, and surface roughness of the gully. Among these parameters, slurry concentration plays the most critical role, but there are few studies on it. This paper focuses on the flow characteristics of slurry with different concentrations, and a series of flume experiments were carried out to obtain the flow characteristics of inundated height, impact force, and velocity in 30%, 40%, 50%, and 60% concentrations. The study confirms that the concentration of slurry has a significant influence on the flow characteristics. Through the experimental study, it is observed that, with the decreasing of slurry concentration, the impact force and velocity of slurry increased in varying degrees; on the contrary, the flow height elevated with the slurry concentration decreasing. The main reason is that the higher the slurry concentration, the higher the static yield stress and viscosity—in varying degrees. The results can provide a detailed understanding of the slurry concentration influence on the flow characteristics, which guides the evacuation time and height downstream.
Monitoring of Sierra Nevada Caves Reveals the Potential for Stalagmites to Archive Seasonal Variability
Authors: Wortham Barbara E. , Montañez Isabel P. , Bowman Kimberly , Kuta Daphne , Contreras Nora Soto , Brummage Eleana , Pang Allison , Tinsley John , Roemer-Baer Greg
Published: December 2021

In the southwestern United States, California (CA) is one of the most climatically sensitive regions given its low (≤250 mm/year) seasonal precipitation and its inherently variable hydroclimate, subject to large magnitude modulation. To reconstruct past climate change in CA, cave calcite deposits (stalagmites) have been utilized as an archive for environmentally sensitive proxies, such as stable isotope compositions (δ18O, δ13C) and trace element concentrations (e.g., Mg, Ba, Sr). Monitoring the cave and associated surface environments, the chemical evolution of cave drip-water, the calcite precipitated from the drip-water, and the response of these systems to seasonal variability in precipitation and temperature is imperative for interpreting stalagmite proxies. Here we present monitored drip-water and physical parameters at Lilburn Cave, Sequoia Kings Canyon National Park (Southern Sierra Nevada), CA, and measured trace element concentrations (Mg, Sr, Ba, Cu, Fe, Mn) and stable isotopic compositions (δ18O, δ2H) of drip-water and for calcite (δ18O) precipitated on glass substrates over a two-year period (November 2018 to February 2021) to better understand how chemical variability at this site is influenced by local and regional precipitation and temperature variability. Despite large variability in surface temperatures and precipitation amount and source region (North Pacific vs. subtropical Pacific), Lilburn Cave exhibits a constant cave environment year-round. At two of the three sites within the cave, drip-water δ18O and δ2H are influenced seasonally by evaporative enrichment. At a third collection site in the cave, the drip-water δ18O responds solely to precipitation δ18O variability. The Mg/Ca, Ba/Ca, and Sr/Ca ratios are seasonally responsive to prior calcite precipitation at all sites but minimally to water-rock interaction. Lastly, we examine the potential of trace metals (e.g., Mn2+ and Cu2+as a geochemical proxy of recharge and find that variability in their concentrations has high potential to denote the onset of the rainy season in the study region. The drip-water composition is recorded in the calcite, demonstrating that stalagmites from Lilburn Cave, and potentially more regionally, could record seasonal variability in weather even during periods of substantially reduced rainfall.
Automatic Detection of Slow Slip Events Using the PICCA: Application to Chilean GNSS Data
Authors: Donoso F. , Moreno M. , Ortega-Culaciati F. , Bedford J. R. , Benavente R.
Published: December 2021

The detection of transient events related to slow earthquakes in GNSS positional time series is key to understanding seismogenic processes in subduction zones. Here, we present a novel Principal and Independent Components Correlation Analysis (PICCA) method that allows for the temporal and spatial detection of transient signals. The PICCA is based on an optimal combination of the principal (PCA) and independent component analysis (ICA) of positional time series of a GNSS network. We assume that the transient signal is mostly contained in one of the principal or independent components. To detect the transient, we applied a method where correlations between sliding windows of each PCA/ICA component and each time series are calculated, obtaining the stations affected by the slow slip event and the onset time from the resulting correlation peaks. We first tested and calibrated the method using synthetic signals from slow earthquakes of different magnitudes and durations and modelled their effect in the network of GNSS stations in Chile. Then, we analyzed three transient events related to slow earthquakes recorded in Chile, in the areas of Iquique, Copiapó, and Valparaíso. For synthetic data, a 150 days event was detected using the PCA-based method, while a 3 days event was detected using the ICA-based method. For the real data, a long-term transient was detected by PCA, while a 16 days transient was detected by ICA. It is concluded that simultaneous use of both signal separation methods (PICCA) is more effective when searching for transient events. The PCA method is more useful for long-term events, while the ICA method is better suited to recognize events of short duration. PICCA is a promising tool to detect transients of different characteristics in GNSS time series, which will be used in a next stage to generate a catalog of SSEs in Chile.
Stable Isotope Analysis of Mammalian Enamel From the Early Pleistocene Site of Madigou, Nihewan Basin: Implications for Reconstructing Hominin Paleoenvironmental Adaptations in North China
Authors: Xu Zhe , Pei Shuwen , Hu Yaowu , de la Torre Ignacio , Ma Dongdong
Published: December 2021

The reconstruction of environmental and climatic changes in the Pleistocene is an essential contribution to our understanding of human evolutionary and behavioral adaptations. Well preserved fluvio-lacustrine sediments at Nihewan basin have yielded a rich record of Early Pleistocene Paleolithic sites and mammalian fossils which provide a unique opportunity for exploring hominin behavior and paleoecology in North China. Taxonomic studies of mammalian fossils have provided important clues to the general environmental setting and landscapes of Early Pleistocene humans in the fluvio-lacustrine basin of Nihewan, but little is known about their isotopic signatures. In this paper, mammal teeth species at the Madigou archaeological site (ca. 1.2 Ma) were selected for bulk and sequential enamel stable isotope (C, O) analysis. Results show a variety of ecological environments, including grassland and sparse forest landscapes, and distinct patterns across taxa. C3-C4 mixed vegetation predominated, but C4 vegetation was also relevant at times. Madigou early humans likely experienced cold/warm or dry/wet fluctuations in this northern China basin. We hypothesize that the environmental fluctuations and diversified landscapes may have driven flexibility in various aspects of early human technological behaviors, and allowed hominins to face the environmental challenges of northern latitudes after the initial expansion from Africa into East Asia at the onset of the Middle Pleistocene Climate Transition.
Impact of Body Mass Index on Static Postural Control in Adults With and Without Diabetes: A Cross-Sectional Study
Authors: Yin Lianhua , Qin Jiawei , Chen Yannan , Xie Jinjin , Hong Cuiping , Huang Jia , Xu Ying , Liu Zhizhen , Tao Jing
Published: December 2021

Aim

The objective of this research was to determine the static postural control differences measured from a force platform in Type 2 diabetes mellitus (T2DM) and healthy control groups with different levels of body mass index (BMI), and detect the static postural control difference between T2DM and healthy control groups stratified by different BMI category. This research also explored the relationship of BMI and static postural performance.

Methods

We recruited 706 participants with T2DM and 692 healthy controls who were sufficiently matched for age, gender, and BMI in this cross-sectional study. The participants were stratified into three groups by BMI: normal weight, overweight, and obesity. All participants performed two-legged static stance postural control assessment on a firm force platform. The Center of Pressure (CoP) parameters were collected under eyes-open and eyes-closed conditions. Mann–Whitney U test was used to compare the static postural control parameters within each BMI category in both groups. The static postural control parameters among different weight groups were compared by Kruskal–Wallis test, post hoc pair-wise comparison were conducted. Generalized linear model was conducted to examine the association between BMI and static postural control parameters while controlling for confounding factors.

Results

Healthy control group had statistical difference in most CoP parameters compared to T2DM group based on all BMI categories. Normal weight participants presented significant difference compared with overweight and/or obesity for total track length (TTL) and velocity of CoP displacements in Y direction (V-Y) under eyes-open condition, and for most CoP parameters under eyes-closed condition in both groups. There were statistically significant correlations between BMI and most static postural control parameters under only eyes-closed condition according to the result of generalized linear model.

Conclusion

T2DM patients had impaired static postural control performance compared to healthy controls at all BMI categories. The findings also indicated the association between BMI and static postural control, where higher BMI individuals showed more static postural instability in both T2DM and healthy controls.
Feasibility of an Inversion Recovery-Prepared Fat-Saturated Zero Echo Time Sequence for High Contrast Imaging of the Osteochondral Junction
Authors: Jang Hyungseok , Ma Yajun , Carl Michael , Lombardi Alecio F. , Chang Eric Y. , Du Jiang
Published: December 2021

Purpose

The osteochondral junction (OCJ) region—commonly defined to include the deep radial uncalcified cartilage, tidemark, calcified cartilage, and subchondral bone plate—functions to absorb mechanical stress and is commonly associated with the pathogenesis of osteoarthritis. However, magnetic resonance imaging of the OCJ region is difficult due to the tissues’ short transverse relaxation times (i.e., short T2 or T2*), which result in little or no signal with conventional MRI. The goal of this study is to develop a 3D adiabatic inversion recovery prepared fat saturated zero echo time (IR-FS-ZTE) sequence for high-contrast imaging of the OCJ.

Method

An IR-FS-ZTE MR sequence was developed to image the OCJ on a clinical 3T MRI scanner. The IR-FS-ZTE sequence employed an adiabatic inversion pulse followed by a fat saturation pulse that suppressed signals from the articular cartilage and fat. At an inversion time (TI) that was matched to the nulling point of the articular cartilage, continuous ZTE imaging was performed with a smoothly rotating readout gradient, which enabled time-efficient encoding of the OCJ region’s short T2 signal with a minimal echo time (TE) of 12 μs. An ex vivo experiment with six cadaveric knee joints, and an in vivo experiment with six healthy volunteers and three patients with OA were performed to evaluate the feasibility of the proposed approach for high contrast imaging of the OCJ. Contrast-to-noise ratios (CNRs) between the OCJ and its neighboring femoral and tibial cartilage were measured.

Results

In the ex vivo experiment, IR-FS-ZTE produced improved imaging of the OCJ region over the clinical sequences, and significantly improved the contrast compared to FS-ZTE without IR preparation (p = 0.0022 for tibial cartilage and p = 0.0019 for femoral cartilage with t-test). We also demonstrated the feasibility of high contrast imaging of the OCJ region in vivo using the proposed IR-FS-ZTE sequence, thereby providing more direct information on lesions in the OCJ. Clinical MRI did not detect signal from OCJ due to the long TE (>20 ms).

Conclusion

IR-FS-ZTE allows direct imaging of the OCJ region of the human knee and may help in elucidating the role of the OCJ in cartilage degeneration.
Signaling Proteins That Regulate Spermatogenesis Are the Emerging Target of Toxicant-Induced Male Reproductive Dysfunction
Authors: Gao Sheng , Wu Xiaolong , Wang Lingling , Bu Tiao , Perrotta Adolfo , Guaglianone Giuseppe , Silvestrini Bruno , Sun Fei , Cheng C. Yan
Published: December 2021

There is emerging evidence that environmental toxicants, in particular endocrine disrupting chemicals (EDCs) such as cadmium and perfluorooctanesulfonate (PFOS), induce Sertoli cell and testis injury, thereby perturbing spermatogenesis in humans, rodents and also widelife. Recent studies have shown that cadmium (e.g., cadmium chloride, CdCl2) and PFOS exert their disruptive effects through putative signaling proteins and signaling cascade similar to other pharmaceuticals, such as the non-hormonal male contraceptive drug adjudin. More important, these signaling proteins were also shown to be involved in modulating testis function based on studies in rodents. Collectively, these findings suggest that toxicants are using similar mechanisms that used to support spermatogenesis under physiological conditions to perturb Sertoli and testis function. These observations are physiologically significant, since a manipulation on the expression of these signaling proteins can possibly be used to manage the toxicant-induced male reproductive dysfunction. In this review, we highlight some of these findings and critically evaluate the possibility of using this approach to manage toxicant-induced defects in spermatrogenesis based on recent studies in animal models.
The Occurrence of Intersex in Different Populations of the Marine Amphipod Echinogammarus marinus in North-West Brittany – A Longterm-Study
Authors: Oetken Matthias , Adler Marissa , Alt Katharina , Bachmann Jean , Dombrowski Andrea , Duhme Franziska , Gabriel Anna-Louise , Grünewald Judith , Jourdan Jonas , Lück Maren , Mensch Carola , Rösch Dominik , Ruthemann Anna , Terres Susanne , Völker Maja Lorina , Wilhelm Ferdinand , Oehlmann Jörg
Published: December 2021

In the past two decades, an increasing body of studies has been published on the intersex phenomenon in separate-sexed crustaceans from marine and freshwater ecosystems. Various causes are being considered that could have an influence on the occurrence of intersex. Besides genetic factors, environmental conditions such as photoperiodicity, temperature, salinity and parasitism, but also environmental pollution with endocrine disrupting chemicals (EDCs) are discussed. As part of a long-term monitoring (2012 – 2020) in north-west Brittany, we recorded the occurrence of intersex in the marine amphipodEchinogammarus marinus. We quantified the intersex incidence at marine and estuarine sites and analyzed the incidence in relation to the endocrine potential of the sediments. Intersex occurred with mean frequencies between 0.87% and 12%. It was striking that the incidence of intersex increased with increasing distance from the sea. Since the highest incidence was observed at the range boundary of this stenohaline species, we assume that intersex is triggered by endocrine potential and increasing stress due to increasing freshwater content − and thus an interplay of different environmental factors.
The U.S. Energy System and the Production of Sustainable Aviation Fuel From Clean Electricity
Authors: Male Jonathan L. , Kintner-Meyer Michael C. W. , Weber Robert S.
Published: December 2021

Jet fuel is relatively small in terms of energy consumption and carbon dioxide emissions (10% of U.S. transportation sector in 2021, expected to increase to 14% by 2050). Still airlines have ambitious goals to reduce their greenhouse footprints from carbon-neutral growth beginning this year to reducing greenhouse gas emission for international flights by 50% by 2050 compared to 2005 levels. The challenge is heightened by the longevity of the current fleet (30–50 years) and by the difficulty in electrifying the future fleet because only 5% of the commercial aviation greenhouse gas footprint is from regional flights that might, conceivably be electrified using foreseeable technology. Therefore, large amounts of sustainable aviation fuel will be needed to reach the aggressive targets set by airlines. Only 3 million gallons (11.4 ML) of sustainable aviation fuel (SAF) (with a heat of combustion totaling about 400 TJ = 0.0004 EJ) was produced in the U.S. in 2019 for a 26 billion gallon per year market (3.6 EJ/year). Fischer-Tropsch and ethanol oligomerization (alcohol-to-jet) are considered for producing SAF, including the use of renewable electricity and carbon dioxide. In sequencing the energy transition, cleaning the U.S. grid is an important first step to have the largest greenhouse gas emissions reduction. While carbon dioxide and clean electricity can potentially provide the SAF in the future, an ethanol oligomerization option will require less energy.
Status and Assessment Method of Nuclear Safety Analysis Software in China
Authors: Gao Xinli , Jing Jianping , Han Xiangzhen , Jia Bin , Tian Xinlu , Liu Fudong , Zhang Chunming
Published: December 2021

In recent years, China’s nuclear power industry has enjoyed a good momentum of development, and related companies have also developed many nuclear analysis software applications. However, as the National Nuclear Safety Administration (NNSA, Chinese nuclear regulatory institution) did not approve any software before 2018, all these software applications were not evaluated formally, so they have not yet been used in reactor safety analysis. In order to solve this problem, in 2018, the National Nuclear Safety Administration started to carry out an engineering applicability evaluation for software developed by Chinese companies. After several years of review, as the first approved Chinese domestic software, core physics analysis software PCM developed by the China General Nuclear Power Group officially passed the software safety evaluation of the China Nuclear Safety Administration. This study will present the basic situation of the development of China’s nuclear power engineering software and introduce the framework, methods, procedures, requirements, and other aspects of China’s software safety evaluation work. The evaluation process and evaluation key issues of PCM software will also be illustrated.
Fuzzy Decision-Based Optimal Energy Dispatch for Integrated Energy Systems With Energy Storage
Authors: Gao Qiang , Zhang Xiaodi , Yang Mixia , Chen Xianqing , Zhou Hongqing , Yang Qiang
Published: December 2021

The integrated energy system is an important strategic direction in the world’s future energy field, which will become the main carrier form of the energy future of human society in the next 30–50 years, directly affecting or even determining the future energy strategy pattern of the world. There are many types of integrated energy system. In the study of optimal dispatching of energy storage, the integrated energy system is modeled according to the energy transmission characteristics of the integrated energy system, which mainly includes the combined cooling, heating and power system and the multi-type energy storage system containing electricity and heat storage. Then, a two-tier optimal scheduling model for an integrated energy system with multiple types of energy storage as the core is established, divided into the day-head scheduling layer and the real-time dispatch layer. At the day-head scheduling layer, an optimization model has been proposed with the minimum cost of the optimization goal and the power network, heating network, cooling network, energy storage operation constraints and carbon constraints as constraints. Then at the real-time dispatch layer, utilize the fuzzy controller to dispatch and control the electric storage system and the thermal storage system. Finally, the verification simulation experiment is carried out in an industrial park. Besides, the energy efficiency, economy and environmental performance before and after the integrated energy system connected to the multi-energy storage device are compared and analyzed, and different scheduling methods are used to compare and prove the advantages of the scheduling method.
Short-Term Power Load Forecasting Under COVID-19 Based on Graph Representation Learning With Heterogeneous Features
Authors: Yu Zhuowei , Yang Jiajun , Wu Yufeng , Huang Yi
Published: December 2021

Since 2020, the COVID-19 has spread globally at an extremely rapid rate. The epidemic, vaccination, and quarantine policies have profoundly changed economic development and human activities worldwide. As many countries start to resume economic activities aiming at a “living with COVID” new normal, a short-term load forecasting technique incorporating the epidemic’s effects is of great significance to both power system operation and a smooth transition. In this context, this paper proposes a novel short-term load forecasting method under COVID-19 based on graph representation learning with heterogeneous features. Unlike existing methods that fit power load data to time series, this study encodes heterogeneous features relevant to electricity consumption and epidemic status into a load graph so that not only the features at each time moment but also the inherent correlations between the features can be exploited; Then, a residual graph convolutional network (ResGCN) is constructed to fit the non-linear mappings from load graph to future loads. Besides, a graph concatenation method for parallel training is introduced to improve the learning efficiency. Using practical data in Houston, the annual, monthly, and daily effects of the crisis on power load are analyzed, which uncovers the strong correlation between the pandemic and the changes in regional electricity utilization. Moreover, the forecasting performance of the load graph-based ResGCN is validated by comparing with other representative methods. Its performance on MAPE and RMSE increased by 1.3264 and 15.03%, respectively. Codes related to all the simulations are available onhttps://github.com/YoungY6/ResGCN-for-Short-term-power-load-forecasting-under-COVID-19.
Hysteretic Implications for Graded Bed Load Sediment Transport in Symmetrical Hydrograph Flows
Authors: Wang Le , Wang Dayu , Cuthbertson Alan , Zhong Deyu , Pender Gareth
Published: December 2021

Differential parametric values associated with bed load sediment transport, that result at the same discharge levels on the rising and falling limbs of a flood hydrograph, are usually defined as bed load hysteresis. This hysteresis in bed load sediment transport rates is of considerable interest in the field of fluvial hydraulics. Within this study, a series of well-defined, symmetrical hydrograph flows are generated over a graded, mobile sediment bed to fully examine the hysteresis of the resulting bed load sediment transport in terms of the threshold of motion, and differential bed load transport rates and bed load yields during the hydrographs. The experiments are conducted in a titling flume without sediment supply specified at the upstream inlet, thereby representing typical river reach conditions immediately downstream of a dam that are exclusively subject to net in-channel bed degradation from sediment transport initiated during flood events. Our results show that the fractional bed load transport of defined fine, medium and coarse size classes within the graded sediment bed generally display clockwise, no/mixed and counter-clockwise hysteresis patterns, respectively, with clockwise hysteresis most commonly found for the coarse size class mobilised by hydrographs with long durations. By contrast, counter-clockwise hysteresis is usually observed for fine size class transported by hydrographs with short durations. Accordingly, the corresponding reference stresses for each size class vary between different hydrographs and are primarily controlled by the hydrograph flashiness (i.e. unsteadiness) and magnitude (i.e. total water work). Moreover, it is shown that the hysteresis effect, particularly for those size classes and hydrograph combinations that result in clockwise and counter-clockwise behaviour, should be fully accounted for when reproducing bed load transport rates using separate-limb based method. Finally, we investigate the relative fractions of the overall bed load yields generated during the rising and falling limbs of all symmetrical hydrographs (i.e. the bed load yield ratio), which are found to be primarily dependent on bed load transport hysteresis. Finally, the relationship between the bed load yield ratio and the ratio of reference stresses for the fractional sediment motion of each size class on both limbs is found to follow a power law.
Evidence for Host Selectivity and Specialization by Epizoic Chelonibia Barnacles Between Hawksbill and Green Sea Turtles
Authors: Boyd Liberty L. , Zardus John D. , Knauer Courtney M. , Wood Lawrence D.
Published: December 2021

Epibionts are organisms that utilize the exterior of other organisms as a living substratum. Many affiliate opportunistically with hosts of different species, but others specialize on particular hosts as obligate associates. We investigated a case of apparent host specificity between two barnacles that are epizoites of sea turtles and illuminate some ecological considerations that may shape their host relationships. The barnacles Chelonibia testudinaria and Chelonibia caretta, though roughly similar in appearance, are separable by distinctions in morphology, genotype, and lifestyle. However, though each is known to colonize both green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) sea turtles, C. testudinaria is >5 times more common on greens, while C. caretta is >300 times more common on hawksbills. Two competing explanations for this asymmetry in barnacle incidence are either that the species’ larvae are spatially segregated in mutually exclusive host-encounter zones or their distributions overlap and the larvae behaviorally select their hosts from a common pool. We indirectly tested the latter by documenting the occurrence of adults of both barnacle species in two locations (SE Florida and Nose Be, Madagascar) where both turtle species co-mingle. For green and hawksbill turtles in both locations (Florida: n = 32 and n = 275, respectively; Madagascar: n = 32 and n = 125, respectively), we found that C. testudinaria occurred on green turtles only (percent occurrence – FL: 38.1%; MD: 6.3%), whereas the barnacle C. caretta was exclusively found on hawksbill turtles (FL: 82.2%; MD: 27.5%). These results support the hypothesis that the larvae of these barnacles differentially select host species from a shared supply. Physio-biochemical differences in host shell material, conspecific chemical cues, external microbial biofilms, and other surface signals may be salient factors in larval selectivity. Alternatively, barnacle presence may vary by host micro-environment. Dissimilarities in scute structure and shell growth between hawksbill and green turtles may promote critical differences in attachment modes observed between these barnacles. In understanding the co-evolution of barnacles and hosts it is key to consider the ecologies of both hosts and epibionts in interpreting associations of chance, choice, and dependence. Further studies are necessary to investigate the population status and settlement spectrum of barnacles inhabiting sea turtles.
Harnessing Genetic Diversity in the USDA Pea Germplasm Collection Through Genomic Prediction
Authors: Bari Md. Abdullah Al , Zheng Ping , Viera Indalecio , Worral Hannah , Szwiec Stephen , Ma Yu , Main Dorrie , Coyne Clarice J. , McGee Rebecca J. , Bandillo Nonoy
Published: December 2021

Phenotypic evaluation and efficient utilization of germplasm collections can be time-intensive, laborious, and expensive. However, with the plummeting costs of next-generation sequencing and the addition of genomic selection to the plant breeder’s toolbox, we now can more efficiently tap the genetic diversity within large germplasm collections. In this study, we applied and evaluated genomic prediction’s potential to a set of 482 pea (Pisum sativum L.) accessions—genotyped with 30,600 single nucleotide polymorphic (SNP) markers and phenotyped for seed yield and yield-related components—for enhancing selection of accessions from the USDA Pea Germplasm Collection. Genomic prediction models and several factors affecting predictive ability were evaluated in a series of cross-validation schemes across complex traits. Different genomic prediction models gave similar results, with predictive ability across traits ranging from 0.23 to 0.60, with no model working best across all traits. Increasing the training population size improved the predictive ability of most traits, including seed yield. Predictive abilities increased and reached a plateau with increasing number of markers presumably due to extensive linkage disequilibrium in the pea genome. Accounting for population structure effects did not significantly boost predictive ability, but we observed a slight improvement in seed yield. By applying the best genomic prediction model (e.g., RR-BLUP), we then examined the distribution of genotyped but nonphenotyped accessions and the reliability of genomic estimated breeding values (GEBV). The distribution of GEBV suggested that none of the nonphenotyped accessions were expected to perform outside the range of the phenotyped accessions. Desirable breeding values with higher reliability can be used to identify and screen favorable germplasm accessions. Expanding the training set and incorporating additional orthogonal information (e.g., transcriptomics, metabolomics, physiological traits, etc.) into the genomic prediction framework can enhance prediction accuracy.