bluuebunny/crossref_metadata_2025 huggingface dataset:
Couples Therapy Delivered Through Videoconferencing: Effects on Relationship Outcomes, Mental Health and the Therapeutic Alliance
Authors: Kysely Andrea , Bishop Brian , Kane Robert Thomas , McDevitt Maryanne , De Palma Mia , Rooney Rosanna
Published: February 2022

Changing technology, and the pervasive demand created by a greater need in the population for access to mental health interventions, has led to the development of technologies that are shifting the traditional way in which therapy is provided. This study investigated the efficacy of a behavioral couples therapy program conducted via videoconferencing, as compared to face-to-face. There were 60 participants, in couples, ranging in age from 21 to 69 years old. Couples had been in a relationship for between 1 to 49 years. The 30 couples were randomly allocated to the face-to-face or videoconferencing group. They all took part in Couple CARE—a couples behavioral education program which promotes self-change in order to increase relationship satisfaction. The six session manualized intervention was offered in an identical manner to all clients, in each condition. Data analysis was based on several questionnaires completed by each couple at pre, post and 3-months follow-up. Results showed that therapeutic alliance ratings did not differ between groups, but increased significantly over time for both groups. Additionally, the results indicated improvements in relationship satisfaction, mental health, and all other outcome scores over time, which did not differ based on condition. This study uniquely contributes to the literature exploring the use of technology to provide therapy. Specifically, the study provides evidence for couples therapy via videoconferencing as a viable alternative to face-to-face interventions, especially for those couples who may not have access to the treatment they require. It is anticipated that the results of this study will contribute to the field of online therapy, and add to fostering confidence in agencies to allow expansion of services conducted via videoconferencing.
An Android for Emotional Interaction: Spatiotemporal Validation of Its Facial Expressions
Authors: Sato Wataru , Namba Shushi , Yang Dongsheng , Nishida Shin’ya , Ishi Carlos , Minato Takashi
Published: February 2022

Android robots capable of emotional interactions with humans have considerable potential for application to research. While several studies developed androids that can exhibit human-like emotional facial expressions, few have empirically validated androids’ facial expressions. To investigate this issue, we developed an android head called Nikola based on human psychology and conducted three studies to test the validity of its facial expressions. In Study 1, Nikola produced single facial actions, which were evaluated in accordance with the Facial Action Coding System. The results showed that 17 action units were appropriately produced. In Study 2, Nikola produced the prototypical facial expressions for six basic emotions (anger, disgust, fear, happiness, sadness, and surprise), and naïve participants labeled photographs of the expressions. The recognition accuracy of all emotions was higher than chance level. In Study 3, Nikola produced dynamic facial expressions for six basic emotions at four different speeds, and naïve participants evaluated the naturalness of the speed of each expression. The effect of speed differed across emotions, as in previous studies of human expressions. These data validate the spatial and temporal patterns of Nikola’s emotional facial expressions, and suggest that it may be useful for future psychological studies and real-life applications.
Compulsory Citizenship Behavior and Its Outcomes: Two Mediation Models
Authors: Liang Huai-Liang , Yeh Tsung-Kai , Wang Chia-Hsuan
Published: February 2022

Employees view compulsory citizenship behavior (CCB) as concessionary behavior they undertake because of pressure exerted by their organizations. This study applies affective events theory to CCB-workplace deviance relationships, and impression management theory to CCB-facades of conformity relationships, to posit that employee emotional exhaustion is an essential mediating factor that effectively explains how CCB contributes to workplace deviance and facades of conformity. This study utilizes two mediation models to investigate whether employees’ CCBs are positively related to their work deviance and false behavior, and how emotional exhaustion mediates those relationships. Two-wave data collected from 655 valid participants (480 males, 175 females; average age of 30.1 years) in a public sector bank and a large private bank in Taiwan supported our hypotheses. We conducted surveys with volunteer employees that included CCB, emotional exhaustion, facades of conformity, and work deviance. The results of this study uncovered statistically significant relationships between CCB and work deviance and between CCB and facades of conformity and revealed that emotional exhaustion significantly mediated these relationships. Implications and directions for future study are discussed.
Adapting a Theory-Informed Intervention to Help Young Adult Couples Cope With Reproductive and Sexual Concerns After Cancer
Authors: Gorman Jessica R. , Lyons Karen S. , Reese Jennifer Barsky , Acquati Chiara , Smith Ellie , Drizin Julia H. , Salsman John M. , Flexner Lisa M. , Hayes-Lattin Brandon , Harvey S. Marie
Published: February 2022

Objective

Most young adults diagnosed with breast or gynecologic cancers experience adverse reproductive or sexual health (RSH) outcomes due to cancer and its treatment. However, evidence-based interventions that specifically address the RSH concerns of young adult and/or LGBTQ+ survivor couples are lacking. Our goal is to develop a feasible and acceptable couple-based intervention to reduce reproductive and sexual distress experience by young adult breast and gynecologic cancer survivor couples with diverse backgrounds.

Methods

We systematically adapted an empirically supported, theoretically grounded couple-based intervention to address the RSH concerns of young couples coping with breast or gynecologic cancer through integration of stakeholder perspectives. We interviewed 11 couples (22 individuals) with a history of breast or gynecologic cancer to review and pretest intervention materials. Three of these couples were invited to review and comment on intervention modifications. Content experts in RSH and dyadic coping, clinicians, and community advisors (one heterosexual couple and one LGBTQ+ couple, both with cancer history) participated throughout the adaptation process.

Results

Findings confirmed the need for an online, couple-based intervention to support young couples experiencing RSH concerns after breast or gynecologic cancer. Qualitative themes suggested intervention preferences for: (1) A highly flexible intervention that can be tailored to couples’ specific RSH concerns; (2) Active steps to help members of a dyad “get on the same page” in their relationship and family building plans; (3) A specific focus on raising partners’ awareness about how cancer can affect body image and physical intimacy; and (4) Accessible, evidence-based information about RSH for both partners. These results, along with feedback from stakeholders, informed adaptation and finalization of the intervention content and format. The resulting virtual intervention, Opening the Conversation, includes five weekly sessions offering training to couples in communication and dyadic coping skills for addressing RSH concerns.

Conclusion

The systematic adaptation process yielded a theory-informed intervention for young adult couples facing breast and gynecological cancers, which will be evaluated in a randomized controlled trial. The long-term goal is to implement and disseminate Opening the Conversation broadly to reach young adult couples with diverse backgrounds who are experiencing RSH concerns in cancer survivorship.
Social Robots for Supporting Post-traumatic Stress Disorder Diagnosis and Treatment
Authors: Laban Guy , Ben-Zion Ziv , Cross Emily S.
Published: February 2022

Post-Traumatic Stress Disorder (PTSD) is a severe psychiatric disorder with profound public health impact due to its high prevalence, chronic nature, accompanying functional impairment, and frequently occurring comorbidities. Early PTSD symptoms, often observed shortly after trauma exposure, abate with time in the majority of those who initially express them, yet leave a significant minority with chronic PTSD. While the past several decades of PTSD research have produced substantial knowledge regarding the mechanisms and consequences of this debilitating disorder, the diagnosis of and available treatments for PTSD still face significant challenges. Here, we discuss how novel therapeutic interventions involving social robots can potentially offer meaningful opportunities for overcoming some of the present challenges. As the application of social robotics-based interventions in the treatment of mental disorders is only in its infancy, it is vital that careful, well-controlled research is conducted to evaluate their efficacy, safety, and ethics. Nevertheless, we are hopeful that robotics-based solutions could advance the quality, availability, specificity and scalability of care for PTSD.
Assessment of Non-physical User Violence and Burnout in Primary Health Care Professionals. The Modulating Role of Job Satisfaction
Authors: Pina David , Llor-Zaragoza Paloma , López-López Reyes , Ruiz-Hernández Jose Antonio , Puente-López Esteban , Galián-Munoz Inmaculada , Martínez-Jarreta Begoña
Published: February 2022

Introduction

Growing concern about workplace violence shows the need for an evaluation in specific contexts in order to identify the particularities of each professional group. The health sector consists of a group of professionals with high exposure to violence, specially from users. There are differences depending on the professional category or unit in which the professional works. In this regard, Primary Health Care (PHC) is characterized by a personalized and continuous patient treatment over time, which is not exempt from cases of violence. Among the commonly studied consequences of these situations are decreases in job satisfaction and burnout.

Objective

The main objective of this study is to analyze the modulating role of job satisfaction in the relationship between non-physical user violence and the onset of burnout.

Methods

Cross-sectional comparative descriptive design. The sample consisted of 574 professionals from 39 PHC centers of the Murcian Health Service. Data were collected using two-stage cluster sampling. For data analysis, descriptive analysis, correlations and stepwise hierarchical regression were used to analyze the interaction between the variables.

Results

Regression analysis draws a model where non-physical violence and low intrinsic and extrinsic job satisfaction act as modulators of non-physical violence, cynicism and emotional exhaustion.

Conclusions

This study provides evidence of the psychological consequences of the perception of user violence in the PHC staff. Furthermore, it is evident that the emergence of burnout syndrome in these professionals is related to exposure to verbal or non-physical violence together with low job satisfaction. In this sense, a circular and bidirectional relationship between the variables studied is proposed as a possible explanatory model.
Sweet Memories or Not? A Comparative Study on Cognitive Impairment in Diabetes Mellitus
Authors: Varghese Sangeetha Merrin , Joy Niva , John Anulekha Mary , George Geomcy , Chandy George Mateethra , Benjamin Anoop Ivan
Published: February 2022

Introduction

Type 2 Diabetes Mellitus is a modern-day epidemic and dementia has been declared as a global challenge. It is, therefore, worthwhile to investigate the effect that Diabetes has on cognition. Although effective screening is routinely carried out for various complications of Diabetes, its effect on Higher Mental Functions is often overlooked.

Methodology

A cross-sectional analytical study to assess Cognitive Impairment was carried out on 800 participants, 400 diabetics and 400 non-diabetics attending a tertiary care center. The Addenbrooke's Cognitive Examination- III was used, which is a validated, highly sensitive tool having a maximum score of 100. Patients with a score < /= 82 were considered to have impaired Cognition. Statistical analysis was done using SPSSv.21. Suitable statistical tests like Mann–Whitney U, t-test, ROC curve and Logistic regression analysis were done.

Results

Cognitive Impairment was present in 63.8% of the diabetics when compared to only 10.8% in the non-diabetics, with an Odds Ratio-8.78 (CI-4.47–17.22). The total ACE score in diabetics [median-82 (IQR-4), mean rank-270.06] was less compared to the non-diabetic patients [median- 85 (IQR-3), mean rank-530.94] (U = 27822, p-0.001). Attention, Memory, Language, and Visuospatial domains were significantly lower in the diabetics compared to the non-diabetics. However, the fluency domain was not affected. Hypertension and the presence of macrovascular diseases were significantly associated with Cognitive Impairment (p < 0.005). Those with Cognitive dysfunction also had higher mean RBS values and longer duration of Diabetes (p-0.001). The cut-off value for RBS (to distinguish people with and without Cognitive Impairment) from ROC curve was 142.5 (AUC = 0.834, Youden's Index-0.586, p-0.001) and for duration of Diabetes was 7.5 years (AUC = 0.847, Youden's Index-0.529, p-0.001).

Conclusion

This paper highlights that Cognitive Impairment exists in a very high proportion of diabetic patients in Kerala. So, it is important that we do an early assessment of cognitive function in diabetes patients and manage them prudently. Early interventions may prove to be beneficial in the long run, considering the burden of diabetes and cognitive dysfunction associated with the disease.
Systematic Review of Biopsychosocial Prognostic Factors for Return to Work After Acute Orthopedic Trauma: A 2020 Update
Authors: Duong Hong Phuoc , Garcia Anne , Hilfiker Roger , Léger Bertrand , Luthi François
Published: February 2022

Objective

To provide updated evidence on prognostic factors for return to work (RTW) in the early and late phases after acute orthopedic trauma from a biopsychosocial perspective.

Methods

A systematic review of articles indexed in the MEDLINE, CINAHL, and Embase databases between 2010 and 2020 was performed. The inclusion criteria were cohort studies of employed populations sustaining acute orthopedic trauma with follow-up data on RTW. Biopsychosocial prognostic factors for RTW must be reported in the multiple regression models and divided into early (≤ 6 months) and late phases (> 6 months) postinjury. Two reviewers performed study selection, assessed the risk of bias and quality using the Quality in Prognosis Studies (QUIPS) tool and the Newcastle–Ottawa Scale (NOS), and extracted data independently.

Results

Thirty articles were included with a follow-up period of 1–58 months. Based on the QUIPS tool, 7 studies (23%) were considered to have a low risk of bias, and 21 studies (70%) were considered to have a moderate risk of bias. Based on the NOS, the quality was high in 87% of the included studies. The RTW rates ranged from 22% to 74% in the early phase and from 44% to 94% in the late phase. In the early phase, strong evidence was found for injury severity. In the late phase, strong evidence was found for age, injury severity, level of pain, self-efficacy, educational level, blue-collar work, and compensation status; moderate evidence was found for recovery expectations and physical workload. There was limited or inconsistent evidence for the other factors.

Conclusion

Based on the levels of evidence, injury severity should be considered as one of the key barriers to RTW in the early and late phases postinjury. This finding underlines the need for serious injury prevention efforts. Our results also emphasize the multifaceted actions of the biopsychosocial model to facilitate RTW: promoting policies for older injured workers, improving access to medical and rehabilitation facilities, and adapting physical workload. Multiple other factors are likely important but require additional high-quality studies to assess their role in the RTW process.
Metacognitive Treatment in Acquired Brain Injury and Its Applicability to Aphasia: A Systematic Review
Authors: Wadams Amanda , Suting Louisa , Lindsey André , Mozeiko Jennifer
Published: February 2022

Purpose

The purpose of this systematic review is to identify the utility of metacognitive therapeutic intervention for persons with acquired brain injury (ABI), with a focus on persons with aphasia.

Methods

A search of six databases resulted in two hundred and sixty-six unique manuscripts relating to the explicit use of metacognitive treatment for people with ABI. Two independent reviewers rated abstracts for inclusion or exclusion of the study given predetermined criteria. Twenty-nine articles, five of which included people with aphasia, were selected for inclusion in this systematic review. SCED+ and PEDro+ rating scales were used to rate the methodological quality of each study.

Results

Methodological quality of the 29 studies that met inclusion criteria ranged from weak to high quality studies. Three -hundred and sixty-nine individuals with ABI took part in the 29 studies. Varying treatment methods were employed. Outcome measures were inconsistent. Metacognitive treatment has been applied to people with aphasia with positive results, but efficacy of the treatment cannot yet be determined.

Conclusions

Metacognitive therapeutic intervention tends to be effective for persons with acquired brain injury (ABI) despite variability between intervention designs and treatment outcomes across studies. Due to so few studies with participants with aphasia, we were unable to draw conclusions regarding the efficacy of metacognitive treatment for people with aphasia. Further research on the efficacy of metacognitive treatment for this population is warranted.
The Problem of Observing Sociotechnical Entities in Social Science and Humanities Energy Transition Research
Authors: Büscher Christian
Published: February 2022

The notion of “sociotechnical” is an important concept for interdisciplinary research on the transformation of the energy supply. Different branches of research agree that the provision, transmission, and distribution of energy are not simply a matter of physics. The transformation of the energy infrastructure is significantly a societal project, carried by technical innovationandsocial change. However, in social science and humanities research the interrelation between technical and social processes is often not explicitly explored, even though the interrelationship is the decisive descriptor that distinguishes sociotechnical entities from their environment. This article examines the merits of enriching the concept of sociotechnical by adding the distinction betweentightandloose couplingsin technical operations and human activities. While tight couplings are necessary to sustain control, they hamper change, and while loose couplings are necessary to adapt and to uphold choice, they increase complexity. Additionally, the article concludes that the introduction of “smart” technologies—an essential vision of the energy transformation—changes the composition of tight and loose couplings. Technical ideas such as machine learning and artificial intelligence go beyond mere automation. We might as well face a new sociotechnical reality. The introduction of intelligence in systems makes more loose couplings necessary. Paradoxically, this allows for new functionality and services by establishing complex operations while at the same time diminishing control by social systems.
Acaricidal and Antioxidant Activities of Anise Oil (Pimpinella anisum) and the Oil’s Effect on Protease and Acetylcholinesterase in the Two-Spotted Spider Mite (Tetranychus urticae Koch)
Authors: El-Sayed Salwa M. , Ahmed Nevin , Selim Samy , Al-Khalaf Areej A. , El Nahhas Nihal , Abdel-Hafez Shams H. , Sayed Samy , Emam Heba M. , Ibrahim Mervat A. R.
Published: February 2022

The two-spotted spider mite, Tetranychus urticae, also known as the red spider, is one of the most harmful pests in agriculture and causes large losses of many crops. These mites have rapidly developed a resistance to many chemical pesticides in recent years. In this study, the essential oil of seeds of the anise plant (Pimpinella anisum) was extracted by hydrodistillation, and the chemical composition of the oil was analyzed. The antioxidant activity of the volatile oil was determined by the DPPH radical scavenging assay. The acaricidal activity of the anise oil, a natural acaricide, was evaluated for its ability to protect green bean plants from mite injury. The two-spotted spiders were spread on green bean seedlings for 1 week; then, different plants were sprayed with different concentrations of anise oil (10, 20, 30, or 40 µL/L). Our results revealed that anethole was the major component of anise oil, at 53.23%. The acaricidal effect of the various concentrations on T. urticae was recorded after 24, 48, and 72 h of treatment. Our findings suggest that anise oil showed significant acaricidal activity against T. urticae in a dose- and time-dependent manner. Anise oil at a concentration of 40 µL/L killed 96.0% of the red spiders after 72 h. Also, all concentrations of anise oil inhibited acetylcholinesterase, and the spiders’ protease activity declined when the plants were treated with 30 or 40 µL/L of anise oil. The concentrations of 10 and 20 µL/L did not significantly affect the protease activity of T. urticae mites. We can conclude that anise oil exhibited acaricidal activity against T. urticae and that this was highly correlated with the inhibition of acetylcholinesterase and protease activities in the mites.
Recovery and Valorization of Bioactive and Functional Compounds from the Discarded of Opuntia ficus-indica (L.) Mill. Fruit Peel
Authors: Scarano Pierpaolo , Tartaglia Maria , Zuzolo Daniela , Prigioniero Antonello , Guarino Carmine , Sciarrillo Rosaria
Published: February 2022

This work focused on the recovery and application of bioactive and functional compounds (BFC) from a waste matrix, the Opuntia ficus-indica (L.) Miller fruit peel (OFP), using different extraction methods: maceration and Extractor Naviglio®. The presence of BFC was initially evaluated by following the extraction yields and antioxidant activity using the Folin-Ciocȃlteu reagent and the DPPH method, respectively. The quali-quantitative content of BFC was also verified with UHPLC/UV-ESI-HRMS profile of OFP extracts. The evaluation of the BFC yields is aimed at using extract components, such as betanins, in the technological application as textile dye based on natural pigments: we evaluated the stability of the extracted dyes, tested the dyeing power and assessed the stability of the dye on the textile material. The results showed different compositions depending on the polarities of the solvents. The ethanol-water mixture extracts obtained using Naviglio method revealed an important presence in terms of total phenolic compound yields. Wool, linen and cotton fibers were chosen for the dyeing tests. Rock alum and lemon juice, two etching methods, were used. Results suggest that the OFP may be of great interest as a natural source of BFC for food, nutraceutical and technological applications in the sustainability context.
Photosynthesis Alterations in Wheat Plants Induced by Herbicide, Soil Drought or Flooding
Authors: Todorova Dessislava , Aleksandrov Vladimir , Anev Svetoslav , Sergiev Iskren
Published: February 2022

The wheat plants were pretreated with the selective herbicide Serrate® (Syngenta) and subsequently subjected to drought or flooding stress for 7 days. The gas exchange parameters, chlorophyll a fluorescence and leaf pigment content were measured. The measurements were performed during the stress period and after 4 days of plants recovery. Herbicide pretreatment did not cause significant alterations in photosynthesis and fluorescence parameters in alone- or combined-treated seedlings. A significant reduction in gas exchange parameters (net photosynthesis rate, stomatal conductance, transpiration rate, and water use efficiency), Fv/Fm and Fv/F0 values during drought or flooding was observed. The disruption of photosynthesis together with reduction in the pigment content was stronger in droughted than flooded plants. When the normal irrigation was restored, the gas exchange and fluorescence parameters tended to increase. The comparative analysis of recovery and resilience indices of photosynthetic traits indicate that the plants subjected to drought recovered better than those subjected to flooding stress.
Influence of Structure of Detrital Food Webs on Fusarium Head Blight of Winter Wheat
Authors: Goncharov Anton A. , Kiseleva Arina D. , Mashkov Kirill A. , Sotnikov Ivan V. , Izvarina Anastasia G. , Ladatko Valery A. , Maygurova Valentina N. , Miroshnichenko Olesya O. , Rozanova Oksana L. , Rapoport Irina B. , Volkova Galina V.
Published: February 2022

Conventional methods for Fusarium head blight (FHB) control are ineffective. A better understanding of the mechanisms linking the abundance of Fusarium species in soil before winter wheat flowering and mycotoxin content in mature grain may help to improve the effectiveness of methods for FHB control. In this study, we established a field experiment aimed to trace the impact of three types of organic mulch with different C:N ratios on the structure of detrital food webs and the manifestation of winter wheat FHB. T2-toxin content in grain was significantly higher in N-poor treatment (52.1 ± 0.2 µg g−1) compared to N-rich treatment (40.4 ± 1.6 µg g−1). The structure of detrital food webs in the studied treatments changed significantly after mulch addition; the abundance of soil saprophages and mycophages increased up to 50%. Based on the results of mixed-effects modeling, the abundance of herpetobionts and soil mesofauna were positively associated with an increase in Fusarium biomass in grain. The increase in the content of T2-toxin in the grain was associated with an increase in the abundance of earthworms and phytophagous macrofauna in the soil. Results suggest the existence of a previously undescribed mechanism for FHB infection by transfer of pathogenic spores by soil invertebrates, while the content of mycotoxins in grain can be triggered by the grazing activity of soil phytophagous invertebrates.
The Effect of Housing System and Gender on Relative Brain Weight, Body Temperature, Hematological Traits, and Bone Quality in Muscovy Ducks
Authors: Krunt Ondřej , Kraus Adam , Zita Lukáš , Machová Karolína , Chmelíková Eva , Petrásek Stanislav , Novák Petr
Published: February 2022

The study was conducted during the summer season (June–August 2020). Two hundred sixty-four 5-week-old sexed Muscovy ducklings were randomly divided into four equal experimental groups by housing system and by gender. Each group had three replicates (22 birds/replicate) in a randomized design experiment. Regarding the hematological traits, the volume of leukocytes was higher in the D group (by 0.34 × 109/L; p < 0.05) than in the S group. Furthermore, body temperature was found to be higher in ducks (by 0.84 °C; p < 0.05) and in the D group (by 0.5 °C; p < 0.05) in comparison with drakes and birds from the S group. Considering relative brain weight, drakes had higher values than ducks (by 0.56 g; p < 0.05), and birds from the S group also manifested higher values (by 0.78 g; p < 0.05). In terms of bone quality, there were no differences in studied parameters of tibia and femur bones regarding housing systems. The results provide valuable evidence of differences in the fattening of intensively bred Muscovy ducks within the housing system but also regarding gender.
Using Mice to Model Human Disease: Understanding the Roles of Baseline Housing-Induced and Experimentally Imposed Stresses in Animal Welfare and Experimental Reproducibility
Authors: Hylander Bonnie L. , Repasky Elizabeth A. , Sexton Sandra
Published: February 2022

Mice are the most common animal used to study disease, but there are real concerns about the reproducibility of many of these experiments. This review discusses how several different sources of chronic stress can directly impact experimental outcomes. Mandated housing conditions induce an underappreciated level of chronic stress but are not usually considered or reported as part of the experimental design. Since chronic stress plays a critical role in the development and progression of many somatic diseases including cancer, obesity, and auto-immune diseases, this baseline stress can directly affect outcomes of such experiments. To study the role of stress in both physical and psychiatric diseases, there has been a proliferation of protocols for imposing chronic stress on mice. For somatic diseases, biomarkers can be used to compare the models with the disease in patients, but to evaluate the validity of psychiatric models, behavioral tests are carried out to assess changes in behavior and these tests may themselves cause an underappreciated degree of additional stress. Therefore, it is important for animal welfare to reduce baseline stress and select the most humane protocols for inducing and assessing chronic stress to obtain the most reliable outcomes.
Porcine Intestinal Apical-Out Organoid Model for Gut Function Study
Authors: Joo Sang-Seok , Gu Bon-Hee , Park Yei-Ju , Rim Chae-Yun , Kim Min-Ji , Kim Sang-Ho , Cho Jin-Ho , Kim Hyeun-Bum , Kim Myunghoo
Published: February 2022

Pig models provide valuable research information on farm animals, veterinary, and biomedical sciences. Experimental pig gut models are used in studies on physiology, nutrition, and diseases. Intestinal organoids are powerful tools for investigating intestinal functions such as nutrient uptake and gut barrier function. However, organoids have a basal-out structure and need to grow in the extracellular matrix, which causes difficulties in research on the intestinal apical membrane. We established porcine intestinal organoids from jejunum tissues and developed basal-out and apical-out organoids using different sub-culture methods. Staining and quantitative real-time PCR showed the difference in axis change of the membrane and gene expression of epithelial cell marker genes. To consider the possibility of using apical-out organoids for intestinal function, studies involving fatty acid uptake and disruption of the epithelial barrier were undertaken. Fluorescence fatty acid was more readily absorbed in apical-out organoids than in basal-out organoids within the same time. To determine whether apical-out organoids form a functional barrier, a fluorescent dextran diffusion assay was performed. Hence, we successfully developed porcine intestinal organoid culture systems and showed that the porcine apical-out organoid model is ideal for the investigation of the intestinal environment. It can be used in future studies related to the intestine across various research fields.
Effect of Dietary Rosemary and Ginger Essential Oils on the Growth Performance, Feed Utilization, Meat Nutritive Value, Blood Biochemicals, and Redox Status of Growing NZW Rabbits
Authors: Elazab Mahmoud A. , Khalifah Ayman M. , Elokil Abdelmotaleb A. , Elkomy Alaa E. , Rabie Marwa M. , Mansour Abdallah Tageldein , Morshedy Sabrin Abdelrahman
Published: February 2022

This study was conducted to assess the impacts of using two essential oils, rosemary and ginger, on growing rabbits’ performance, carcass traits, meat composition, blood biochemicals, and the redox status of growing New Zealand White (NZW) rabbits. A total of 120 unsexed NZW rabbits, 42-days-old, were assigned randomly to five experimental groups (n = 24, 6 replicates with 4 rabbits each). The first group received a basal diet (control), the second to fifth groups were dietary supplemented daily with rosemary essential oil (REO) and ginger essential oil (GEO) at doses of 0.25 and 0.5% for each supplementation (REO-0.25, REO-0.5, GEO-0.25, and GEO-0.5), respectively. The growth traits were studied for 7 weeks, from the 7th to the 13th week of the rabbits’ age. The results revealed that final body weight, weight gain, and average daily gain increased significantly (p < 0.01) in the REO-0.5 and GEO-0.5 treatments compared to the control group. Daily feed intake decreased (p = 0.005) in essential oil treatments. Meanwhile, the feed conversion ratio improved significantly (p = 0.001) in REO and GEO at the high doses compared to the control group. The weight percentages of liver and giblets increased (p < 0.001) with both treatments of REO and GEO compared to the control group. The dietary supplementation with REO and GEO did not affect (p > 0.05) the meat composition of Longissimus dorsi and hind leg muscles. Meanwhile, REO and GEO supplementation significantly decreased cholesterol levels in the rabbit meat. Thiobarbituric acid reactive substance concentrations decreased by 10 and 15% in the meat of REO-0.5 and GEO-0.5 treatments, respectively, compared to the other groups. In the same trend, REO and GEO treatments induced a significant (p = 0.001) reduction in the plasma cholesterol concentrations and triglycerides compared to the control. The total antioxidant capacity increased by 7.60% and the malondialdehyde decreased by 11.64% in the plasma of GEO-0.5 treatment than the control. Thus, the dietary supplementation of REO and GEO have a beneficial effect in improving the productivity and meat quality of growing rabbits.
Time Series Ovarian Transcriptome Analyses of the Porcine Estrous Cycle Reveals Gene Expression Changes during Steroid Metabolism and Corpus Luteum Development
Authors: Park Yejee , Park Yoon-Been , Lim Seok-Won , Lim Byeonghwi , Kim Jun-Mo
Published: February 2022

The porcine estrous cycle is influenced by reproductive hormones, which affect porcine reproduction and result in physiological changes in the reproductive organs. The ovary is involved in ovulation, luteinization, corpus luteum development, and luteolysis. Here, we aimed to provide a comprehensive understanding of the gene expression patterns in porcine ovarian transcriptomes during the estrous cycle through differentially expressed genes profiling and description of molecular mechanisms. The transcriptomes of porcine ovary were obtained during the estrous cycle at three-day intervals from day 0 to day 18 using RNA-seq. At seven time points of the estrous cycle, 4414 DEG were identified; these were classified into three clusters according to their expression patterns. During the late metestrus and diestrus periods, the expression in cluster 1 increased rapidly, and steroid biosynthesis was significant in the pathway. Cluster 2 gene expression patterns represented the cytokine–cytokine receptor interaction in significant pathways. In cluster 3, the hedgehog signaling pathway was selected as the significant pathway. Our study exhibited dynamic gene expression changes with these three different patterns of cluster 1, 2, and 3. The results helped identify the functions and related significant genes especially during the late metestrus and diestrus periods in the estrous cycle.
Thyroid Hormones in Hemolymph of Red King Crabs from the Barents Sea
Authors: Dvoretsky Alexander G. , Tipisova Elena V. , Alikina Viktoria A. , Elfimova Aleksandra E. , Dvoretsky Vladimir G.
Published: February 2022

Only a few studies have reported the presence and levels of thyroid hormones in invertebrates. At the same time, the administration of these hormones has been shown to be a profitable method for inducing growth in crustaceans. Knowledge of thyroid hormone concentrations is required to involve this approach in crustacean aquaculture. For this reason, we examined hemolymph samples of the invasive red king crab Paralithodes camtschaticus from the Barents Sea population for the levels of triiodothyronine (T3) and thyroxine (T4). Radioimmunoassays indicated that T3 ranged from 0.26 to 1.55 μg dL–1, whereas T4 varied from 52 to 203 μg dL–1. Adult crabs demonstrated higher levels of T3 than juveniles, while juvenile crabs had higher levels of T4 in comparison to larger conspecifics. The thyroxine levels were consistent throughout the study period. In contrast, significant seasonal variations were found for T3. In immature crabs, the maximum concentration was found in October, whereas in adult crabs, the highest level of T3 was registered in August. Most likely, these results are explained by changes in physiological conditions of the crabs associated with their molting calendar and migration activity. Our data may have important implications for aquaculture of Paralithodes camtschaticus in the Barents Sea.
A Supervised Classification of Children with Fragile X Syndrome and Controls Based on Kinematic and sEMG Parameters
Authors: Piatkowska Weronika Joanna , Spolaor Fabiola , Romanato Marco , Polli Roberta , Huang Alessandra , Murgia Alessandra , Sawacha Zimi
Published: February 2022

Fragile X syndrome (FXS) is caused by pathologic expansions of the CGG repeat polymorphic region of the FMR1 gene. There are two main categories of FMR1 mutations, “premutation” and “full mutation”, that are associated with different clinical phenotypes, and somatic mosaicism can represent a strong FXS phenotype modulator. FXS is the leading cause of inherited intellectual disability and autism, and it is characterized by musculoskeletal manifestations such as flexible flat feet, joint laxity and hypotonia. The former have been associated with altered joint kinematics and muscle activity during gait. The aim of this study was to use gait analysis parameters to classify FXS children from healthy controls and, within FXS children with full mutation, to classify children with mosaicism. Seven supervised machine learning algorithms were applied to a dataset of joint kinematics and surface electromyographic signals collected on twenty FXS children and sixteen controls. Results showed that the k-NN algorithm outperformed in terms of accuracy (100%) in classifying FXS children from controls, while CN2 rule induction obtained the best accuracy (97%) in classifying FXS children with mosaicism. The proposed pipeline might be used for developing assisted decision-making systems aiming at identifying and treating the musculoskeletal alterations associated with FXS.
Numerical Parametric Studies on the Stress Distribution in Rocks around Underground Silo
Authors: Kim Sun-Hoon , Kim Kwang-Jin
Published: February 2022

The underground silo was constructed as a facility for the disposal of low- and intermediate-level radioactive waste. It is divided into three parts: the upper-dome core, the lower-dome core, and the cylindrical-space core. Numerical parametric studies on the stress distribution occurring in the surrounding rocks around the underground silo are presented in this paper. It is assumed that the soil layer was distributed to a depth of −4.3 m from the ground level, the weathered rocks were distributed to a depth of −9.5 m from the bottom of the soil layer, and the rocks were distributed in the lower part of the weathered rocks. A 2D axial symmetric finite element model was considered for the numerical analysis of the underground silo. A 3D finite element model was used to verify the reliability of the 2D axial symmetric model. Finite element analysis was carried out under various ratios of in situ horizontal stress to vertical stress (Ko). The numerical results obtained through these analyses include detailed stress states in the p–q and octahedral planes at key locations of finite element models around an underground silo. Contours of safety factor distributions are also presented to evaluate the overall structural safety of the surrounding rock mass, which is the main supporting body of the underground silo.
Laser Cooling beyond Rate Equations: Approaches from Quantum Thermodynamics
Authors: Murphy Conor N. , Toledo Tude Luísa , Eastham Paul R.
Published: February 2022

Solids can be cooled by driving impurity ions with lasers, allowing them to transfer heat from the lattice phonons to the electromagnetic surroundings. This exemplifies a quantum thermal machine, which uses a quantum system as a working medium to transfer heat between reservoirs. We review the derivation of the Bloch-Redfield equation for a quantum system coupled to a reservoir, and its extension, using counting fields, to calculate heat currents. We use the full form of this equation, which makes only the weak-coupling and Markovian approximations, to calculate the cooling power for a simple model of laser cooling. We compare its predictions with two other time-local master equations: the secular approximation to the full Bloch-Redfield equation, and the Lindblad form expected for phonon transitions in the absence of driving. We conclude that the full Bloch-Redfield equation provides accurate results for the heat current in both the weak- and strong- driving regimes, whereas the other forms have more limited applicability. Our results support the use of Bloch-Redfield equations in quantum thermal machines, despite their potential to give unphysical results.
Pressure Drop and Particle Settlement of Gas–Solid Two-Phase Flow in a Pipe
Authors: Lin Wenqian , Li Liang , Wang Yelong
Published: February 2022

Particle settlement and pressure drop in a gas–solid two-phase flow in a pipe with a circular cross-section are studied at mixture inlet velocities (V) ranging from 1 m/s to 30 m/s, particle volume concentrations (αs) ranging from 1% to 20%, particle mass flows (ms) ranging from 5 t/h to 25 t/h, and particle diameters (dp) ranging from 50 μm to 1000 μm. The momentum equations are based on a two-fluid model and are solved numerically. Some results are validated through comparison with the experimental results. The results showed that the gas and particle velocity distributions are asymmetrical around the center of the pipe and that the maximum velocity point moves up. The distance between the radial position of the maximum velocity and the center line for the gas is larger than that for the particles. The particle motion lags behind that of the gas flow. The particle settlement phenomenon is more serious, and the particle distribution on the cross-section is more inhomogeneous as the V, αs, and ms decrease and as dp increases. It can be divided into three areas according to the pressure changes along the flow direction, and the distinction between the three areas is more obvious as the αs increases. The pressure drop per unit length increases as the V, αs and ms increases and as dp decreases, Finally, the expressions of the settlement index and pressure drop per unit length as functions of V, αs, ms, and dp are derived based on the numerical data.
Design of the Test Section for the Experimental Validation of Antipermeation and Corrosion Barriers for WCLL BB
Authors: Utili Marco , Alberghi Ciro , Candido Luigi , Di Fonzo Fabio , Papa Francesca , Venturini Alessandro
Published: February 2022

Tritium permeation into the Primary Heat Transfer System (PHTS) of DEMO and ITER reactors is one of the challenging issues to be solved in order to demonstrate the feasibility of nuclear fusion power plants construction. Several technologies were investigated as antipermeation and corrosion barriers to reduce the tritium permeation flux from the breeder into the PHTS. Within this frame, alumina coating manufactured by Pulsed Laser Deposition (PLD) and Atomic Layer Deposition (ALD) are two of the main candidates for the Water Cooled Lithium Lead (WCLL) Breeder Blanket (BB). In order to validate the performance of the coatings on relevant WCLL BB geometries, a mock-up was designed and will be characterized in an experimental facility operating with flowing lithium-lead, called TRIEX-II. The present work aims to illustrate the preliminary engineering design of a WCLL BB mock-up in order to deeply investigate permeation of hydrogen isotopes through PHTS water pipes. The permeation tests are planned in the temperature range between 330 and 500 °C, with hydrogen and deuterium partial pressure in the range of 1–1000 Pa. The hydrogen isotopes transport analysis carried out for the design and integration of the mock-up in TRIEX-II facility is also shown.
Modelling Fire Risk Exposure for France Using Machine Learning
Authors: Gualdi Baptiste , Binet-Stéphan Emma , Bahabi André , Marchal Roxane , Moncoulon David
Published: February 2022

Wildfires generating damage to assets are extremely rare in France. The peril is not covered by the French natural catastrophes insurance scheme (law of 13 July 1982). In the context of the changing climate, Caisse Centrale de Réassurance—the French state-owned reinsurance company involved in the Nat Cat insurance scheme—decided to develop its knowledge on the national exposure of France to wildfire risks. Current and future forest fires events have to be anticipated in case one of the events threatens buildings. The present work introduces the development of a catastrophe loss risk model (Cat model) for forest fires for the French metropolitan area. Cat models are the tools used by the (re)insurance sector to assess their portfolios’ exposure to natural disasters. The open-source national Promethée database focusing on the South of France for the period 1973–2019 was used as training data for the development of the hazard unit using machine learning-based methods. As a result, we observed an extension of the exposure to wildfire in northern areas, namely Landes, Pays-de-la-Loire, and Bretagne, under the RCP 4.5 scenario. The work highlighted the need to understand the multi-peril exposure of the French country and the related economic damage. This is the first study of this kind performed by a reinsurance company in collaboration with a scholarly institute, in this case EURIA Brest.
A Comparison between Non-Localized Post-Activation Performance Enhancements Following Resistance Exercise for the Upper and the Lower Body
Authors: Bartolomei Sandro , Lanzoni Ivan Malagoli , Fantozzi Silvia , Cortesi Matteo
Published: February 2022

The aim of the present investigation was to compare the acute non-localized post-activation performance enhancement (PAPE) of an exercise protocol involving either the upper or the lower body muscles. Twenty-four resistance trained men participated in the present study and were randomly assigned to an upper body (UB) or to a lower body (LB) group. Both groups tested for upper and lower body power (bench press throw (BPT) and countermovement jump power (CMJP) tests). Participants in the UB group were tested pre and post a high-intensity (HI) and a high-power (POW) bench press protocol while participants in the LB group performed a HI squat and a jump session (POW). A significant group × time interaction was found for CMJP in HI (p = 0.012). Post hoc tests revealed that CMJP was elevated in UB group only (+1.6%; p = 0.025). No other significant interactions were detected. Results of this study indicate that a non-localized PAPE on the lower body may be induced by a HI bench press protocol while a HI squat protocol may not increase upper body power. In particular, the squat protocol performed in the present study (5 sets of 1 rep) may be too demanding to produce a non-localized PAPE.