bluuebunny/crossref_metadata_2025 huggingface dataset:
Diagnostic Performance of p16ink4a in the Presence of E6/E7 mRNA in Formalin-fixed Paraffin Embedded Specimens: A Cross-sectional Study
Authors: Singh Ravindra Pratap , Verma Surendra Kumar
Published: September 2024

Introduction: Clinically significant High-risk Human Papillomavirus (HR-HPV) infection in Oral Cavity Squamous Cell Carcinoma (OCSCC) is not clearly defined. There is uncertainty regarding the true impact of HPV-related variability (ranging from 0% to 100%) when using the GP5+/GP6+/MY09/11 (L1) primer. The identification of HPV DNA in tumours is insufficient to establish a causal viral association; therefore, a gold standard test is required to determine HPV’s role in malignant cells by identifying the transcripts of the E6/E7 mRNA viral oncogene and the p16ink4a biomarker. Aim: To assess the diagnostic performance of p16ink4a in the presence of E6/E7 mRNA in Formalin-fixed, Paraffin-embedded (FFPE) specimens. Materials and Methods: This cross-sectional study was conducted in the Department of Pathology at JIPMER in Puducherry, India, from February 2019 to April 2022. Combined molecular markers p16ink4a and E6/E7 mRNA (considered the gold standard) from FFPE specimens of newly diagnosed Squamous Cell Carcinoma (SCC) were analysed to identify biologically and clinically relevant Infections using Immunohistochemistry (IHC) and real-time Polymerase Chain Reaction (PCR). Results: This study indicates that tissue samples with p16 positive expression (score of 2 or higher) showed the presence of HPV E6/E7 mRNA, with a sensitivity of 100% and a specificity of 91.67%. The relationship between p16ink4a and E6/E7 mRNA expression in all OCSCC cases was found to be significant (p-value=0.003). Conclusion: The study found a significant relationship between mRNA and p16ink4a overexpression in tongue SCC. This finding suggests that alterations in mRNA expression levels are associated with changes in p16ink4a expression in tongue cancer. Such understanding could potentially aid in the development of diagnostic or therapeutic strategies targeting these molecular pathways in tongue SCC.
Early Onset Felty Syndrome in Rheumatoid Arthritis: A Case Report
Authors: Kumar Abhishek , Harikrishnan P , Manrai Manish , Kumar Abhinav
Published: September 2024

Felty syndrome is a rare and serious complication of Rheumatoid Arthritis (RA), characterised by a triad of RA, splenomegaly (enlarged spleen), and neutropenia (low neutrophil count). RA, a systemic autoimmune inflammatory disease marked by synovitis, commonly occurs in the fifth or sixth decade of life and often affects patients with a history of RA exceeding ten years. It is more prevalent in the Caucasian population due to the underexpression of the Human Leukocyte Antigen (HLA)-DR4 gene in African Americans. Felty syndrome requires the presence of neutropenia, defined as an Absolute Neutrophil Count (ANC) below 2000 cells/mm³, alongside splenomegaly and long-standing RA. Hereby, the authors present a case report of a 44-year-old female with a three-year history of RA, who presented with ascites for nine months and was found to have portal hypertension. Her diagnosis of Felty syndrome was confirmed based on the triad of RA, neutropenia, and splenomegaly, highlighting the importance of early recognition and management to prevent severe complications such as infections and systemic issues like portal hypertension.
A Pathologist Visit to the Zoo: A Review on Animal Eponyms in Pathology
Authors: Umarji Seema A , Padmapriya K , Gouri SR Mangala
Published: September 2024

The world of pathology encompasses a broad spectrum of diseases beyond the boundaries of specialties, with each disease having specific and interesting gross and microscopic features. Some of these features are pattern-based, while others are eponyms that compare them to objects, food, animals, etc., due to their striking resemblance and quick recall. Medical nomenclature is of vital importance, and it has significantly evolved historically, with etymological roots from Latin, Greek, and Roman languages of ancient times to the current internationally uniform codes of English and other modern languages. Eponyms are valuable medical literary epithets that have been used across specialties and include animal names, food names, discoverer names, geographic references, and more. The subject of pathology, in particular, has immense use of eponyms as they are valuable tools for assisting in adult learning, or andragogy. The specialty of pathology is unique in its complex patterns and diagnostic algorithm, always in need of alternate systems to arrive at quick and accurate diagnosis. Also known as intuitive thinking or reflex thinking, pattern viewing and eponyms trigger a reflex recognition system, reducing recall time and aiding in precise diagnosis. The present article aimed to review the terminological phenomenon of animal eponym usage in the context of pathological diagnosis.
Influence of Different Head and Neck Positions on Oropharyngeal Leak Pressure with the LMA Protector Airway: A Cross-over Study
Authors: . Deepika , Prafulla Ravipati , Taxak Susheela , Govil Vasudha , Lallar Anushmita , James Elizabeth
Published: September 2024

Introduction: The assessment of seal pressure or leak pressure in supraglottic devices has commonly been used to confirm device functionality and for research purposes regarding laryngeal mask airways (LMAs). When used for controlled ventilation, the LMA Protector provides a better oropharyngeal seal pressure. Materials and Methods: In this cross-over study, thirty-five patients of either sex, aged 18 to 70 years, American Society of Anaesthesiology (ASA) class I and II, with a weight range of 50-70 kg, scheduled for elective surgery under general anaesthesia where a supraglottic airway device was indicated, were enrolled. In all 35 patients, the position of the patient’s head and neck was changed from neutral to flexion, extension, and right and left lateral rotation. The primary objective of the study was to compare the differences in oropharyngeal leak pressure (OLP) with changes in head and neck positions using the LMA Protector airway device. Results: The mean age of the participants was 42.26 years, and the average weight was 63.34 kg. The LMA Protector was placed in 18 females and 17 males. A Mallampati grade of I was noted in 16 patients, while a grade of II was observed in 19 patients. The OLP achieved in this study was higher, providing adequate ventilation and oxygenation in all positions. The OLP and tidal volume in flexion were higher compared to the seal pressure in the neutral position (31.89 cm H2O, SD 5.080 cm H2O vs. 34.49 cm H2O, SD 4.804 cm H2O, p<0.05). No significant difference in OLP was observed when changing to different head positions, i.e., in extension, right lateral, and left lateral positions. Conclusion: The LMA Protector is an effective device in terms of achieving high oropharyngeal seal pressure. It produced better oropharyngeal seal pressure in the flexion position compared to the neutral position.
A Case of Haemorrhages caused by Multiple Myeloma Induced Factor Deficiencies: A Bleeding Enigma
Authors: Lakshman Abhijith Lakshman , Suman Febe Renjitha , Vasugi Gramani Arumugam , Ramdoss Uma Lakshmi
Published: September 2024

A 74-year-old male patient presented with anaemia, thrombocytopenia, and extensive haemorrhages over both thighs and back. A coagulation work-up revealed markedly elevated Prothrombin Time (PT) and activated Partial Thromboplastin Time (aPTT). Reduced functional values of fibrinogen as determined by clot-based assay (126.4 mg/dL), as well as factor V (17.5%) and factor X (27.8%), were noted. These abnormalities were corrected by mixing studies with normal plasma. Neoplastic plasma cells were seen in the bone marrow biopsy, while abdominal fat biopsies showed no amyloid deposition. Serum electrophoresis and immunofixation were positive for monoclonal gammopathy. The patient was diagnosed with multiple myeloma complicated by multiple acquired factor deficiencies. The patient showed a complete response within six months of treatment, which included bortezomib and cyclophosphamide. Multiple myeloma is a monoclonal proliferation of plasma cells that results in increased production of free immunoglobulin light chains, resulting in increased paraproteins. These paraproteins bind with and inhibit various clotting factors, resulting in their inactivation and depletion. Factor X deficiency in these cases is usually attributed to concomitant amyloidosis, whose fibrils bind to Factor X. However, these deficiencies can occur in the absence of amyloidosis as well. While various reports of this entity have highlighted isolated factor deficiencies including factors V, VII, IX, and X, multiple factor deficiency is a rare occurrence, with no other cases being reported from southern India.
Cytodiagnosis of Metastatic Thyroid Follicular Carcinoma in Left Iliac Bone with Occult Primary: A Case Report
Authors: Goswami Shreya Giri , Bhake Arvind
Published: September 2024

Bone metastasis of Follicular Thyroid Carcinoma (FTC) is an aggressive complication. Typically, follicular carcinoma remains confined to the thyroid gland, with distant metastasis occurring in 4-15% of cases. The lungs are the most frequent site of metastasis, followed by bone (2-13%). A peculiar clinical manifestation of FTC observed is the metastasis with the primary still remaining clinically occult. Solitary bone metastases are exceedingly rare and can easily be misdiagnosed as primary bone tumours on clinicoradiological assessment. This causes delays in the diagnosis and management. In these cases, the cytomorphology of cells on diagnostic Fine Needle Aspiration (FNA) was found to be useful in suggesting the primary origin of FTC. Distant bone metastases from follicular carcinoma on histopathological examination from biopsy specimens have been reported in the literature. The cytodiagnosis and cytomorphology of metastatic FTC by Fine Needle Aspiration Cytology (FNAC) are seldomly described in the literature. Involvement of the iliac bone in the metastasis of FTC is uncommon. The FNAC of such a bony lesion helps immensely not only in the diagnosis of the metastasis but also in suggesting the primary malignancy. Authors, hereby report a clinicopathological profile of one such rare case of a 74-year-old female with cytodiagnosis of FTC with no clinical thyroid swelling. The cytodiagnosis made from the primary lytic lesion involving the left iliac bone led to the retrospective radiological and histopathological work-up of the thyroid gland, confirming the occult primary carcinoma of the thyroid gland. The present case highlights the rare presentation and cytomorphological details of metastatic thyroid follicular carcinoma.
Cold Urticaria: An Under-recognised Cause of Perioperative Anaphylaxis
Authors: . Monika , . Himani , Chhikara Monica , Jaswal Akshay , Kataria Vivek Singh
Published: September 2024

Anaphylaxis is a rare but severe, potentially life threatening allergic reaction which commonly presents with cutaneous to multisystem involvement. The overall incidence rate of anaphylaxis during general anaesthesia varies greatly, ranging from 1:20,000 to 1:38. Cold urticaria, subset of chronic urticaria, is characterised with the presence of localised wheals to systemic and anaphylactic reactions. Physical urticaria can be due to physical stimuli such as friction, pressure, sun exposure and cold. Cold urticaria mainly comprises of physical urticaria rather which ranges from 3-33.8% with a higher incidence in cold climate. A case of physical cold urticaria was reported during perioperative period in a 40-year-old male who was posted in urology OT for a planned Ureteroscopy (URS). On pre-anaesthetic evaluation, patient’s medical, surgical and personal history found to be inconclusive. Patient was fit for surgery under ASA grade 1 and advised to be kept nil per orally for six hours for solid food and two hours for clear fluid. At the conclusion of surgery, patient developed shivering and was managed with warm blankets and radiant heater. After some time, patient develop wheals over chest, abdomen and lower back. Patient was managed with warm fluids, warming the patient, maintaining ambient OT temperature and drugs like injection pheniramine, epinephrine and dexamethasone to prevent catastrophic reaction. Through evaluation during preoperative period plays a key role in anticipation of these kind of untoward events. This case report describes the occurrence and management of this rare condition.
Evaluation of Drug Prescription using WHO Prescription Indicators at a Tertiary Care Teaching Hospital, Raipur Chhattisgarh, India: A Cross-sectional Study
Authors: Jaiswal Shikha , Chandra Nalini Singh , Agrawal Manju , Sheohare Raka Sheohare , Mishra Ajitesh
Published: September 2024

Introduction: Prescription patterns significantly impact healthcare quality and patient outcomes. Evaluating these patterns using World Health Organisation (WHO) indicators helps identify areas for improvement and ensures rational drug use. The present study was conducted to evaluate prescription patterns via WHO indicators, aiming to uncover insights, identify discrepancies and enhance prescription quality within the institute for improved healthcare outcomes. Aim: To evaluate drug prescription patterns using WHO prescription indicators in the prescriptions of outdoor patients at a tertiary care teaching hospital in Chhattisgarh, India. Materials and Methods: A cross-sectional study was conducted in the Department of Pharmacology at Department of Pharmacology, Pt. J.N.M. Medical College, Raipur, Chhattisgarh, India, from July 15, 2023, to October 15, 2023 (a duration of 3 months), involving 1,000 outpatient prescriptions at Dr. Bhimrao Ambedkar Memorial Hospital (BRAM), Raipur, Chhattisgarh. Ethical clearance was obtained from the Institutional Ethics Committee (IEC) (No./MCI/Ethics/2023/45 Raipur, dated 12/07/2023). Data were collected using a digital form and analysed statistically. Parameters evaluated included the average number of medicines per prescription, the percentage of drugs prescribed by generic name, the percentage of prescriptions containing antibiotics, the percentage of prescriptions containing injections, and the percentage of drugs prescribed from the Essential Drug List (EDL). Descriptive statistics were used to summarise the data, and analysis was performed using Microsoft (MS) Excel 2021. Results: The present study included 1,000 prescriptions. A total of 4,320 medicines were prescribed across these prescriptions, with 2,853 (66.04%) prescribed by their generic name. Injections accounted for 483 (11.18%) prescriptions, while antibiotics were included in 663 (15.34%) prescriptions. Additionally, 2,973 (68.81%) medicines were prescribed from the EDL. The average number of medicines prescribed per prescription was 4.3. Conclusion: The study revealed that while certain aspects of prescription practices aligned with WHO indicators, notable discrepancies persisted. The percentage of antibiotics prescribed per prescription was in accordance with WHO guidelines. Strategic measures are needed to enhance prescription practices and align them more closely with WHO guidelines.
Chevron Bone and OS Penis in Dog: Structural and Functional Anatomy
Authors: S Tamilselvan , S Chemmalar , Sivagnanam S.
Published: September 2024

The study was conducted to record the structural and functional anatomy of chevron bone and os penis in dogs. The chevron bone along with coccygeal vertebra and os penis were collected from four adult dogs by natural wet maceration technique. The chevron bone was alphabet capital “V” or “Y” shaped bone which consisted of three processes namely, two lateral process and one ventral process. The two lateral processes were articulated with the haemal process of coccygeal vertebrae to form haemal canal. The chevron bones functionally protect the middle coccygeal artery which has important clinical role in assessing the animal health. The os penis was a heterotrophic bone situated inside the penis. It was triangular in outline with a base, a body and an apex. The body consisted of three surfaces and three borders. The ventral surface was made up of deep urethral sulcus which accommodated and protected the urethra.
Report of (Tetragonula iridipennis) Smith, 1854 Pollination on Flowering Plant [Bryophyllum pinnatum (Lam.)] in New Delhi, India
Authors: Gouda M. N. Rudra , K B Chethan Kumar , H Jeevan , M Yogananda , H G Shashank , L.M.B Ashwini , B Tulasi
Published: September 2024

Research was carried out in New Delhi, India, where the discovery of Tetragonula iridipennis, also known as the Kelulut or Trigona bee, as a pollinator of Bryophyllum pinnatum (Lam.) (Crassulaceae), commonly referred to as "Mother of Thousands," was recorded and species identification was mainly carried out by molecular method. T. iridipennis has not been previously documented as a pollinator of B. pinnatum, a succulent plant known for its distinctive method of vegetative propagation. This finding presents a unique addition to the study of pollination ecology, demonstrating the adaptability of these bees in supporting the reproduction of a plant species that has not been previously documented. The research also emphasises the significance of comprehending the interactions between bees and plants, as well as the importance of safeguarding both bee species and bryophyllum plants in their respective ecosystems. The recent discovery of this connection highlights the wide range of hosts that T. iridipennis can infect and its importance in supporting biodiversity and plant reproduction. Additional insights into this distinctive pollination relationship may be obtained through further research, which can enhance our understanding of the dynamics between pollinators and plants.
A Brief Review on the Emerging Role of Bacterial Pigments: From Industrial Uses to Novel Therapeutic Applications
Authors: N S Delna , Mishra Renu , Praksh Anil
Published: September 2024

Pigments produced by microorganisms are in high demand due to their safe, non-toxic, and biodegradable characteristics in both industrial and pharmaceutical fields. Using bacteria for pigment production offers several advantages, such as a short life cycle and ease of genetic modification. Numerous studies have highlighted that soil bacteria significantly contribute to the production of coloured pigments as secondary metabolites. Bacterial pigments are well-known for their antimicrobial, antioxidant, and anticancer properties, offering new therapeutic opportunities for the development of novel drugs. However, compared to fungal pigments, most bacterial pigments are still in the research and development stage. Hence, efforts to intensify bacterial pigment production are essential to make them readily available on the market at a low cost for various applications. This review article sheds light on bacterial pigments and their various applications based on the data available in the literature.
A Sustainable Future : The Importance and the Development of Sustainable Finance In the 21st Century
Authors: Korpes Alev Dilek Aydın
Published: September 2024

The environmental problems that have emerged along with rapidly increasing global warming in recent years have made the concept of sustainability an important issue in the world in general. This fact has been largely effective in increasing the sensitivity of businesses and leading corporations to the environmental problems. This situation has led businesses to implement environmental friendly policies and invest in these areas, both interms of efficient use of natural resources and minimizing the damage to the environment.
With the increasing global warming, climate change has recently become one of the most important problems in the world, and many countries have started to make efforts to reduce the risk of climate change. The inadequacy of environemental measures taken so far has necessitated the search for solutions in different areas regarding this issue. In this sense, financial institutions also contribute to climate awareness by following environmental policies in their activities and developing products that prioritize the environment.
The concept of sustainable finance has led to the development of various financial products and to the development of financing projects aimed at protecting the environment. While the concept of sustainable finance is perceived as the responsibility of public authorities, the private sector has also been included in the process with the Sustainable Development Goals in the UN 2030 Agenda. There is no doubt that sustainable banking and finance practices, especially the implementation of sustainability practices by banks and other corporations in developing countries will contribute to the development of these countries considerably.
In this study, it is aimed to draw attention to the global climate crisis, to emphasize the relationship between finance and the environment, and to contribute to the increase of applications in this field in the world by introducing green financing tools. One of the important functions of sustainable finance against the climate crisis is the evolution of companies funded within the scope of ESG criteria into a production process that tends to be sensitive to carbon emissions, as well as acquiring social and governance sensitivity. In this way, an economic process can be carried out on the axis of environmental and social awareness. In addition to combating climate change, it can be an important supporter in achieving the Sustainable Development Goals.
Enhancing Freshness: A Comparative Study of Innovative Preservation Methods for Berries and Leafy Greens
Authors: Mehmood Muhammad Kamran , Maqbool Danish , Rizwan Hammad , Bibi Sadia , Nawaz Muhammad Ali , Hussain Muhammad Zeeshan , Sultan Muhammad Meelad , Sajjad Zain Ul
Published: September 2024

Berry and leafy greens are some of the most difficult types of food to preserve and ensure they be fresh for as long as possible. The present research assesses the impact of modern preservation procedures namely High Pressure Processing (HPP), Active Packaging, and Edible Coatings with the conventional preservation methods such as Refrigeration and Freezing on microbial load, textural properties, color, and nutrient retention of perishable produce. Purchased strawberries, blueberries, raspberries and spinach, lettuce and kale are possible candidates for preservation methods; the treatments employed in the study included a control no treatment group. Microbial load, texture firmness, colour, Vitamin C and Iron content and sensory evaluation parameters were used in this study. Those found out that HPP technology was the most efficient, cutting through microbial deposits to crops, and retaining texture, color, and nutrients in better ways than the ordinary washing techniques or even the control methods. Overall, the microorganism load was the lowest in HPP-treated produce, e. g., strawberries with 2. 5 × 10² CFU/g, while strawberries treated with HPP had the best texture firmness of 8. 5 N, and better retention of Vitamin C and Iron compared with other treatments. Both Active Packaging and Edible Coatings were also observed to be effective in a way that was superior to inactive packaging but not as effective as HPP. However, it was observed that sensory acceptability score was higher in HPP treated samples, so it enhances the quality as well as the appealing aesthetic value of fresh perishables. From this research, it becomes clear that there is a high possibility for innovative methods to extend the shelf life of the perishable crops while positive contributing to the widely useful methods and efficient preservation techniques of perishable crops.
The Effect of Using Compost on Cowpea Productivity in the Semi-Arid Region of Brazil
Authors: Alves Debora Viviane , Linhares Paulo César Ferreira , Santos Maria Eduarda Pereira dos , Mendonça Leonardo Angelo , Dantas Rita de Cássia dos Santos , Santos Mateus de Freitas Almeida dos , Freitas Bruno Lucas Medeiros de , Dantas Thiffany Louhanne Alves , Godeiro Maria Cecília Costa , Alves Lunara de Sousa , Silva Jezimiel Oliveira da , Silva Uilma Laurentino da , Rodrigues Walter Martins , Souza Bianca Alves
Published: September 2024

The production of cowpea is part of the agricultural activities of farmers in the semi-arid region of Brazil, being important in the aspect of acquiring income and source of food, in this sense, the use of rooster tree (Calotropis procera) mixed with cattle manure in cowpea productivity in the semi-arid region of Brazil. The experiment was conducted from August to November 2023 at the Rafael Fernandes experimental farm, in the district of Alagoinha (5º03'37 "S, 37º23'50" W), northwest of Mossoró, State of Rio Grande do Norte, Brazil, with an area of 400 hectares. The experimental design used was randomized complete blocks in a 4 x 2 factorial scheme, with three replications. The first factor consisted of four quantities of cattle manure (0.0; 1.1; 2.2 and 4.4 kg m-2), the second factor being the presence and absence of rooster tree. Each plot consisted of dimensions of 2.2 m x 1.2 m, with a total area of 2.64 m2. Each plot had eight holes with three plants, making a population of 90,900 plants ha-1. After harvesting the dried pods, they were stored in plastic bags, identified and taken to the DCAF/UFERSA Post-Harvest laboratory, where the following characteristics were measured: pod length, expressed in cm, number of pods plant-1, expressed in plant-1 units, weight of five pods, expressed in grams, weight of 100 grains, expressed in grams and dry grain productivity, expressed in kg ha-1. The highest cowpea productivity was observed at a dose of 4.4 kg m-2, with a maximum value of 2,858.85 kg ha-1. The presence of rooster tree did not influence any of the characteristics evaluated. The use of cattle manure is an option for farmers in the semi-arid region.
Impact of Varietal Demonstrations on the Productivity and Sustainability in Rice (Oryza sativa L.) at Villupuram District of Tamil Nadu, India
Authors: Ganapathy S. , Gomadhi G. , Kanchanarani R. , Senthilkumar M.
Published: September 2024

Popularization of newly released variety in a new environment was important to increase the productivity and sustainability on a particular location. Keeping this inview, field demonstrations were carried out at farmer’s field by introducing drought tolerant rice variety TKM 15. The variety was released by Rice Research Station, (TNAU), Thirurkuppam, during 2022. A total of ten, field demonstrations were conducted at farmer’s field organized by Krishi Vigyan Kendra, Villupuram, and Tamil Nadu during Kharif 2023. The farmers cultivating variety ADT 37was used as the check variety (farmer’s practice). An average yield of 4,925kg.ha-1 was recorded from TKM 15 demonstrations which showed 7.75% increase over the farmers cultivating variety (4570kg.ha-1). The farmers have obtained additional revenue of Rs. 10,100ha-1from TKM 15 demonstrations, which may motivate the farmers to adopt TKM 15 variety with the improved rice production technology. The Front-Line Demonstration on new variety introduction, effectively influenced the attitudes, skills, and knowledge related to improved or recommended practices in paddy cultivation, fostering adoption. It also enhanced the relationship between farmers and scientists, fostering mutual confidence.
Rapid Assessment of Riparian Vegetation at Calyawan River, Barangay Andap, Municipality of New Bataan, Davao de Oro Province, Philippines
Authors: Jr Genaro F. Alderite , Ventura Rex B.
Published: September 2024

Aims: The aim of this study was to examine the vegetation found along the riverbanks of the Calyawan River in Barangay Andap, Municipality of New Bataan, Province of Davao de Oro, Philippines using the transect walk method. The goals were to determine the variety and abundance of the flora along the riverbank, evaluate the conservation status of the discovered plant species, and suggest measures for safeguarding and preserving the riparian environment.
Study Design: The study employed a transect walk method to carry out an initial survey of the riparian vegetation. A 1-kilometer transect was conducted along the riverbanks of the Calyawan River to gather qualitative and quantiatative measurements of plant diversity. The evaluation took place on December 20, 2022. This approach facilitated a quick and systematic examination of the vegetation along the riverbanks.
Place and Duration: The study was carried out on the riverbanks of the Calyawan River in Barangay Andap, Municipality of New Bataan, Province of Davao de Oro. The fieldwork was conducted on December 20, 2022. The region can be reached by using the Compostela-Montevista-Mati Boundary Road.
Methodology: The transect walk approach was employed to record the riparian flora during the survey. Plant species were identified and photographed, but no tangible plant specimens were collected. The obtained data encompassed the identification of fern and angiosperm taxa and families encountered along the transect. The conservation status assessments were conducted using the pre-existing classifications of the species.
Results: A rapid assessment revealed the presence of 49 distinct genera of ferns and angiosperm plants belonging to 30 different families along the transect. Out of these, 74% of the riparian plant genera that were identified were found to be endemic to the region. The assessment of the riparian plants' conservation status indicated that 37% were classed as vulnerable, 30% as least concern, 22% as other threatened species, 10% as endangered, and 1% were not categorized. Although the sample time was short, these findings emphasize the abundance and importance of the riparian flora in terms of conservation.
Conclusion: The initial study conducted along the riverbanks of Calyawan River indicated a notable variety of plants that grow along the river, with a large number of species that are unique to that area. Many identified plants have a conservation status that emphasizes the necessity for heightened sampling efforts and monitoring in order to gain a more comprehensive understanding of and safeguard this rich ecosystem. Considering the clear human-induced stresses on the forest fragments in the region, it is advisable to enhance and broaden conservation efforts in order to protect the riparian vegetation and guarantee its ongoing existence.
Level of Aflatoxins in Selected Cereals in Ekiti State, Nigeria
Authors: T. O. Femi-Ola, , O. T. Fajilade, , T. R. Omodara,
Published: September 2024

Aflatoxins are family of toxins produced by certain fungi found on agricultural crops such as maize (corn), peanuts, cottonseed, and nuts. The ingestion of such mycotoxin contaminated grains by animals and human beings has enormous public health significance. This has necessitated the evaluation of effects of Asparagus africanus (Lam - whole plant) extracts on aflatoxins production by fungi isolated from selected foods in Ekiti State, Nigeria. A total of 240 food samples were collected across the 16 Local Government areas in rural open markets. The food samples analyzed were: Igbemo rice Oryza sativa igbemo curtivar), maize (Zea mays) and groundnut (Arachis hypogaea). Aflatoxin-producing fungi and non aflatoxin producing fungi were isolated and identified using morphological method of identification on PDA and microscopic with lactophenol cotton blue stain. The molecular identification of the isolates was done using internal transcribe spacer (ITS) region. A total of 70 samples that contain Aspergillus spp were subjected to ELISA test to quantify and identify types of aflatoxins that were present in them. It was revealed that four types of aflatoxins were present in the food samples (AFB1, AFB2, AFG1, and AFG2). Also, different species of isolates were obtained from the samples viz: Aspergillus flavus, Aspergillus niger, Rhizopus stolonifer, Mucor mucedo, A. parasiticus, Neurospora crassa, Botrytis cinerea, Fusarium oxyporum and Penicillium oxalicum. Aspergillus flavus was prevalent among the isolates. The molecular identification showed three of the isolates were Aspergillus flavus with different percentage of ITS4 rDNA identity. The level of aflatoxins in some of the food samples analyzed is of great concern because of its ability to cause disease (in man and animal) and these toxins are introduced mostly during storage, that is, post-harvest.
Content Analysis of Knowledge and Adoption of Climate-resilient Cotton Production Practices among Dryland Farmers
Authors: Chennamadhava M. , Praveena P. L. R. J. , Lakshmi T. , Reddy P. Maheswara
Published: September 2024

Cotton is a vital crop for smallholder farmers in India, particularly in the rainfed regions of Andhra Pradesh. The dryland farming is vulnerable to climate change, water scarcity, and soil degradation, leading to reduced crop yields and decreased livelihood security. Critical interventions like conservation agriculture, micro irrigation, and integrated nutrient management are essential to address these challenges. Despite their importance, the adoption of these interventions among dryland farmers remains limited. This study aimed to assess the knowledge and adoption of critical interventions among dryland farmers in cotton cultivation. The results showed that 75-100% of farmers had knowledge about basic practices like sowing time, seed rate, and spacing, while 50-75% knew about pest and disease management practices. However, only 25-50% were aware of drought mitigating measures, and 0-25% knew about advanced practices like micro irrigation and anti-transpirants. The adoption of critical interventions was also low, with 75-100% adoption of basic practices, 50-75% adoption of pest and disease management practices, and 25-50% adoption of drought mitigating measures. The study highlights the need for awareness and training programs to improve the knowledge and adoption of critical interventions among dryland farmers. The findings suggest that there is a significant knowledge and adoption gap among dryland farmers, particularly regarding advanced practices. Addressing this gap is crucial to enhancing the livelihoods of dryland farmers and promoting sustainable cotton production in the region.
The Effects of Organic Fertilizers and NPK on Growth and Yields of Cowpea (Vigna unguiculata. L) in Kano Plains Kisumu, Kenya
Authors: Balah Oyata E. B. , Thomas Rewe , Ogallo Leo
Published: September 2024

Excessive use of inorganic mineral fertilizers as an agronomic practice has resulted in the depletion of soil organic matter of soils of many regions thereby rendering such soils infertile and unable to maintain sustainable production. The use of organic amendments provides the immediate strategy for compensating for soil carbon depletion thereby reducing land degradation. The purpose of this study was to evaluate the effects of six organic amendments on the growth and yield of Cowpea (Vigna unguiculata L) as compared to the conventional use of inorganic fertilizer NPK. The organic amendments were Boom Max, Ecoplanting, Evergrow, Dung slurry, Filter mud, and Market waste slurry applied to the soil at 8.33 t/ha against NPK (9:15:20) at 250 kg/ha. The experiment was laid in a RCBD. Data on growth and yields was collected on 10 tagged plants per plot. The results showed differences among treatments. Mature composts (Boom Max, Ecoplanting, and Evergow) resulted in a significantly (p< 0.05) higher germination percentage (53.49% on average) compared to that of Filter mud (41.50%) and the slurries (34.18% on average) while all the composts resulted in 25% higher number of branches on the average in season two as compared to the bio-slurries.  In season three, Boom Max gave the highest germination percentage (41.02%) which was significantly higher than those of Evergrow (29.80 %), Filter mud and Dung slurry (27.18% on average) and NPK and Market waste slurry (5.64% on average) but not different from that of Ecoplanting. Five of the amendments had significantly better germination rates than NPK by an average of 20%. There were no significance differences (P≤0.05) in growth parameters between the mature composts and the NPK fertilizer, suggesting that the mature composts were equally efficient in nutrient supply to the crop as did the NPK fertilizer for plant growth. The application of organic amendments produced significant improvements in fresh leaf yields of the cowpea crop, with the highest yield recorded from the Boom Max applied plot (5781 kg on average), which was not different from others in seasons one and three but significantly different from that of NPK in season three. It can be concluded that depending on choice or type of amendment, organic manures are capable of producing similar or better results of crop growth and yields as the inorganic fertilizers.
Growth Parameters and Protein Content of Maize as Influenced by Sowing Methods and Different Levels of Nitrogen
Authors: Singh Bhanu , Siddiqui Saba , Sachan Dhruvendra Singh , Yadav Sushil Kumar , Ahmad Shabbeer
Published: September 2024

The field experiment was carried out at Research Farm, Integral University, Lucknow, Uttar Pradesh, India during Kharif season, 2021. The experiment envisages to study the effect of Sowing methods and different Nitrogen levels on yield and attributes of maize crop. The experiment was laid down in the Split Plot Design and the treatments were replicated three times. The maximum plant height of 56.92 cm, 235.17 cm and 237.16 cm was observed in the ridge method of sowing at knee height stage, tasselling stage and at harvest stage, respectively. Among nitrogen levels, the maximum plant height of 57.61 cm, 235.29 cm and 238.38 cm was observed at knee height stage, tasselling stage and at harvest stage, respectively in the treatment (N5) 25% N through granular urea + 75% N through Nano-urea. Among nitrogen levels, the maximum DMA of 86.30, 425.36 and 1287.73 g sqm-1 day-1 was observed at knee height stage, tasselling stage and at harvest stage, respectively in the treatment (N5) 25% N through granular urea + 75% N through Nano-urea. At harvest stage number of leaves per plant were highest in the S1 (14.33) and N5 (16.13) treatment. Among nitrogen levels, the maximum leaf area index (LAI) of 1.55, 2.49 and 1.9 was observed at knee height stage, tasselling stage and at harvest stage, respectively in the treatment N5. Among sowing methods, highest protein content was found in the ridge method (10.14%) followed by flat method (9.81%) and broadcast method (9.42%) of sowing. The protein yielded maximum of 586.15 kg ha-1 in the N5 treatment. All parameters showed better in granular urea application rather than nano-urea, when used solely.
Development of New Variant Beer Using Papaya
Authors: Hemanth Pappoppula , Kumar Sourabh
Published: September 2024

Beer becoming one of the ancient and most widely enjoyed beverages globally, is witnessing a growing popularity in the form of functional brews. Nevertheless, there is a constraint on conducting a study on the impact of incorporating functional adjuncts at different stages of the production process and how this affects the final physical, chemical, and sensory properties. The current study examines the ingredients employed and their degree of incorporation to produce a functional beer with bioactive compounds, improved antioxidant activity, and increased flavor qualities. Beer has been a trend with an infusion of fruit in the form of Pieces or extracts. In addition, they were successfully introduced into the various steps like wort boiling, fermentation, maturation, and packaging processes to enhance the consumer perception point of view and the mutual benefits of health such as heart diseases and Stroke as improvement of Bone Density. Beer samples were made with the addition of papaya pulp in different ratios in 17 combinations/replicants.
Comprehensive Overview of Plant Germplasm Augmentation: Strategies, Techniques and Applications
Authors: Abhishek GJ , Ragi Shridhar , Bhatt Kailash Chandra , Kumar Rajneesh
Published: September 2024

Plant genetic resources (PGR) are fundamental for crop improvement and conservation, encompassing a wide range of genetic materials including land races, modern varieties, and wild relatives. Effective germplasm exploration is essential for advancing plant genetic resources and ensuring agricultural sustainability. Traditional germplasm collection faces challenges such as remote locations, limited material availability, and transport issues. This review explores various strategies to address these challenges, including coarse-grid and fine-grid survey methods, multi-species versus species-specific collecting missions, and the implications of breeding systems on germplasm collection. It highlights the benefits of Geographic Information Systems (GIS) in managing PGR, from incentivizing mapping strategies to optimizing conservation and evaluation processes. Additionally, the review emphasizes the role of in vitro techniques in overcoming limitations of traditional methods, detailing field and laboratory procedures for successful in vitro collection. By integrating these approaches, the review aims to enhance the efficiency and effectiveness of PGR management, ensuring the preservation of genetic diversity for future generations.
Effectiveness of Plant Oils for Bio-intensive Control of Uzi Fly Infestation in Mulberry Silkworm (Bombyx mori): A Comparative Study
Authors: Naan Toko , Sut Rubi , Kashyap Bidisha , Dutta Roshmi Borah , Saikia Monimala , Gogoi Inee , Saikia Hemanta
Published: September 2024

A study was carried out to assess the effect of plant oils against the uzi fly (Exorista sorbillans Weidemann) and the mulberry silkworm (Bombyx mori Louis), at three different concentrations: 0.2, 0.4, and 0.8 percent. Among the treatments, 0.8 % of neem (Azadirachta indica) oil recorded reduced number of eggs laid (fecundity) (40.33), egg hatchability (46,00 %), and maggot recovery (34.00 %), followed by karanj (Pongamia pinnata) oil (54.00, 52,67 and 50.00%, respectively) and was statistically superior to control (114.33, 93.00% and 92%) and Nesolynx thymus (107.67, 90.00 and 89.00%). Significantly higher maggot (larvae) mortality was observed with 0.8 per cent neem oil (65.33 %), followed by karanj oil (35.33 %) compared to castor oil (24.67 %), and control treatment (11.00%). Spraying plant oil from neem and karanj, also drastically reduced the pupation rate %, adult emergence%, adult longevity, and fecundity of the uzi fly.
Association between Physico Biochemical Parameters of Ber (Ziziphus mauritiana Lamk.) cv. Umran under Cold Storage
Authors: Shivran Jitendra Singh , Bairwa L. N. , Choudhary M. R. , Jat Mohan Lal , Shivran Mamta
Published: September 2024

Aim: The objective of the study was to examine the relationship between the physical and biochemical attributes of Indian jujube or ber (Ziziphus mauritiana Lamk.) cultivar Umran, affected by treatments applied after harvest and stored under cold storage conditions.
Place and Duration: This investigation was conducted at Horticulture Department of SKN Agriculture University, Jobner in the month of February to March, 2019.
Methodology: The fruits treated with calcium chloride (CaCl2) (0.5, 1.0 and 1.5%) and gibberellic acid (GA3) (20, 40 and 60ppm) while being maintained in cold storage. The experiment was carried out utilizing a Completely Randomized Design (CRD), consisting of seven treatments and three replications. The study evaluated multiple attributes of fruits, such as physiological weight loss, marketability, pulp percentage, total soluble solids, titratable acidity, ascorbic acid, total sugars and reducing sugars.
Results: The correlation analysis revealed a highly significant positive correlation between the weight of the fruit and various quality measures, such as marketability (r = 0.959), pulp percent (r =0.846), total soluble solids (r =0.947), titratable acidity (r =0.998), ascorbic acid (r =0.991), total sugars (r =0.980) and reducing sugars (r =0.963). While, a strong negative association was observed between the fruit's weight and PLW (r =-0.947). The linear regression model provides additional evidence for this relationship revealed that changes in fruit weight were responsible for a substantial amount of the differences observed in the evaluated quality attributes. The values of the coefficients of determination (r²) varied between 0.747 and 0.996.
Conclusion: The strong correlations and regression model provide valuable insights for improving post-harvest handling and storage practices, leading to enhanced preservation of fruit quality during cold storage.
Effect of Cow Urine and Culture Filtrates of Trichoderma isolate S-13 on Fungal Pathogens of Basmati Rice
Authors: Maurya Priya Devi , Khilari Kamal , Mishra Prashant , Singh Ramesh , Singh Gaje , Yadav Manoj Kumar
Published: September 2024

Rice (Oryza sativa L.) is the principle food crop for majority population of the world. Basmati rice is a unique crop of Himalaya comprising good quality characters and aroma. Basmati rice is being infected by numerous diseases. To manage these diseases, fungicides being used for years. This leads to the residual effects of fungicides on grains and soils so there is needs for alternate approaches to the manage disease. Hence use of cow urine and Trichoderma isolate S-13 is considered as the sustainable approach for management of important diseases of basmati rice. In this study we used different concentration of cow urine, Trichoderma isolate S-13, and Propiconazole (control check) against brown spot and bakanae disease of rice. Among these the maximum mycelial inhibition observed in Trichoderma isolate S-13 @15per cent (66.33per cent) followed by cow urine @15per cent (52.59 per cent). In Bakanae disease the maximum inhibition observed in Trichoderma isolate S-13 @ 15per cent (58.89 per cent) followed by cow urine@15per cent (49.26 per cent).
Insecticidal and Anti-Feedant Activity of Clerodendrum Extracts on Growth and Development of Castor Spiny Caterpillar, Ariadne merione
Authors: N. Dharani Priya, , L Srinithi, , K Sudharsan. , C Subhika. , K Subicksha. , M Tharun. , P Thenprakash.
Published: September 2024

The castor spiny caterpillar Ariadne merione (F.) is a major lepidopteran pest affecting castor (Ricinus communis) crops mainly by causing defoliation which in turn reduces the photosynthesis and yield. The conventional pest control methods have been based on chemical control which although has been effective, has its drawbacks of being hazardous to the environment and human health besides leading to resistance by the pests. The present work aims at investigating the possibility of using Clerodendrum inerme, a plant belonging to the Lamiaceae family, as an eco-friendly solution for pest management. Insecticidal and anti-feedant efficacy of C. inerme leaf extracts, which were prepared using solvents hexane, acetone, and petroleum ether, was tested against A. merione larvae using leaf dip bioassay under no-choice situation. The findings showed that the hexane extract had the highest insecticidal (73%) and anti-feedent (97%) action. The petroleum ether extract also showed moderate insecticidal (50%) and anti-feedant activity (48%) but not as potent as the acetone extract having insecticidal and anti-feedant action of 60 and 74 per cent respectively. The results imply that C. inerme extracts, especially the hexane extract, could be used as botanical insecticides in IPM programs as a natural replacement for chemical insecticides.