bluuebunny/crossref_metadata_2025 huggingface dataset:
Higher risk of pre-eclampsia and other vascular disorders with artificial cycle for frozen-thawed embryo transfer compared to ovulatory cycle or to fresh embryo transfer following in vitro fertilization
Authors: Epelboin Sylvie , Labrosse Julie , De Mouzon Jacques , Devaux Aviva , Gervoise-Boyer Marie-José , Hesters Laetitia , Jonveaux Philippe , Levy Rachel , Sermondade Nathalie , Fauque Patricia , Pessione Fabienne
Published: May 2023

Background

Risks of maternal morbidity are known to be reduced in pregnancies resulting from frozen embryo transfer (FET) compared to fresh-embryo transfer (fresh-ET), except for the risk of pre-eclampsia, reported to be higher in FET pregnancies compared to fresh-ET or natural conception. Few studies have compared the risk of maternal vascular morbidities according to endometrial preparation for FET, either with ovulatory cycle (OC-FET) or artificial cycle (AC-FET). Furthermore, maternal pre-eclampsia could be associated with subsequent vascular disorders in the offspring.

Methods

A 2013-2018 French nationwide cohort study comparing maternal vascular morbidities in 3 groups of single pregnancies was conducted: FET with either OC or AC preparation, and fresh-ET. Data were extracted from the French National Health System database. Results were adjusted for maternal characteristics and infertility (age, parity, smoking, obesity, history of diabetes or hypertension, endometriosis, polycystic ovary syndrome and premature ovarian insufficiency).

Results

A total of 68025 single deliveries were included: fresh-ET (n=48152), OC-FET (n=9500), AC-FET (n=10373). The risk of pre-eclampsia was higher in AC-FET compared to OC-FET and fresh-ET groups in univariate analysis (5.3% vs. 2.3% and 2.4%, respectively, P<0.0001). In multivariate analysis the risk was significantly higher in AC-FET compared to fresh-ET: aOR=2.43 [2.18-2.70], P<0.0001). Similar results were observed for the risk of other vascular disorders in univariate analysis (4.7% vs. 3.4% and 3.3%, respectively, P=0.0002) and in multivariate analysis (AC-FET compared to fresh-ET: aOR=1.50 [1.36-1.67], P<0.0001). In multivariate analysis, the risk of pre-eclampsia and other vascular disorders were comparable in OC-FET and fresh-ET: aOR=1.01 [0.87-1.17, P= 0.91 and aOR=1.00 [0.89-1.13], P=0.97, respectively).Within the group of FET, the risks of pre-eclampsia and other vascular disorders in multivariate analysis were higher in AC-FET compared to OC-FET (aOR=2.43 [2.18-2.70], P<0.0001 and aOR=1.5 [1.36-1.67], P<0.0001, respectively).

Conclusion

This nationwide register-based cohort study highlights the possibly deleterious role of prolonged doses of exogenous estrogen-progesterone supplementation on gestational vascular pathologies and the protective role of the corpus luteum present in OC-FET for their prevention. Since OC-FET has been demonstrated not to strain the chances of pregnancy, OC preparation should be advocated as first-line preparation in FET as often as possible in ovulatory women.
Assessment of riverbank filtration performance for climatic change and a growing population
Authors: Abd-Elaty Ismail , Kuriqi Alban , Ganayem Hala M. , Ahmed Ashraf , Saleh Osama K. , Garrote Luis
Published: May 2023

Riverbank filtration (RBF) consists of green drinking water production in many regions and is used as a pre-treatment phase. This study investigates the performance of the RBF in the Nile delta, Egypt, for climate change and population growth scenarios of 2030, 2040, and 2050. This study presents a new method for predicting the sharing of riverbanks considering three cases: i) the river stage controlling the water levels in the river, ii) increasing RBF pumping, and iii) changing the groundwater levels. This last scenario is achieved by changing the general head in the MODFLOW model. The results showed that RBF sharing (RBFS) is a proportion of the river leakage inflow, in which the decrease of the river stage due to the influence of climate change reduced the river leakage inflow and RBFS. In addition, increasing RBF pumping, decreasing RBF pumping, and lowering the groundwater levels due to the increase in the future drinking water pumping for the population growth increased the river leakage inflow and RBFS. Finally, combining the three cases decreased RBFS in the coming years of 2030, 2040, and 2050, respectively, due to more groundwater sharing than the river inflow. The results show that the water budget is a good tool to investigate RBFS compared with MT3D results. This technique can reduce the cost of water quality collection and analysis; moreover, it will help with the estimation of RBF and save time compared with solute transport modeling.
Swine acute diarrhoea syndrome coronavirus (SADS-CoV) Nsp5 antagonizes type I interferon signaling by cleaving DCP1A
Authors: Huang Hai-xin , Zhao Chen-chen , Lei Xiao-xiao , Zhang Xin-yu , Li Yu-ying , Lan Tian , Zhao Bao-peng , Lu Jing-yi , Sun Wen-chao , Lu Hui-jun , Jin Ning-yi
Published: May 2023

Swine acute diarrhoea syndrome coronavirus (SADS-CoV), which is a recently discovered enteric coronavirus, is the major aetiological agent that causes severe clinical diarrhoea and intestinal pathological damage in pigs, and it has caused significant economic losses to the swine industry. Nonstructural protein 5, also called 3C-like protease, cleaves viral polypeptides and host immune-related molecules to facilitate viral replication and immune evasion. Here, we demonstrated that SADS-CoV nsp5 significantly inhibits the Sendai virus (SEV)-induced production of IFN-β and inflammatory cytokines. SADS-CoV nsp5 targets and cleaves mRNA-decapping enzyme 1a (DCP1A) via its protease activity to inhibit the IRF3 and NF-κB signaling pathways in order to decrease IFN-β and inflammatory cytokine production. We found that the histidine 41 and cystine 144 residues of SADS-CoV nsp5 are critical for its cleavage activity. Additionally, a form of DCP1A with a mutation in the glutamine 343 residue is resistant to nsp5-mediated cleavage and has a stronger ability to inhibit SADS-CoV infection than wild-type DCP1A. In conclusion, our findings reveal that SADS-CoV nsp5 is an important interferon antagonist and enhance the understanding of immune evasion by alpha coronaviruses.
Biocontrol potential of Chitinophaga flava HK235 producing antifungal-related peptide chitinocin
Authors: Kim Da Yeon , Han Jae Woo , Lee Jin Woo , Kim Bomin , Kim Yeong Seok , Kim Heung-Tae , Choi Gyung Ja , Kim Hun
Published: May 2023

Botrytis cinerea is a necrotrophic fungal pathogen with an extremely broad host range, causing significant economic losses in agricultural production. In this study, we discovered a culture filtrate of bacterial strain HK235, which was identified as Chitinophaga flava, exhibiting high levels of antifungal activity against B. cinerea. From the HK235 culture filtrate, we isolated a new antimicrobial peptide molecule designated as chitinocin based on activity-guided fractionation followed by characterization of the amino acid composition and spectroscopic analyses. The HK235 culture filtrate and chitinocin completely inhibited both conidial germination and mycelial growth of B. cinerea at a concentration of 20% and 200 μg/mL, respectively. In addition to antibiosis against B. cinerea, the active compound chitinocin had a broad antifungal and antibacterial activity in vitro. When tomato plants were treated with the culture filtrate and chitinocin, the treatment strongly reduced the development of gray mold disease in a concentration-dependent manner compared to the untreated control. Here, considering the potent antifungal property in vitro and in vivo, we present the biocontrol potential of C. flava HK235 for the first time.
Advances in cholera research: from molecular biology to public health initiatives
Authors: Walton Madison G. , Cubillejo Isabella , Nag Dhrubajyoti , Withey Jeffrey H.
Published: May 2023

The aquatic bacterium Vibrio cholerae is the etiological agent of the diarrheal disease cholera, which has plagued the world for centuries. This pathogen has been the subject of studies in a vast array of fields, from molecular biology to animal models for virulence activity to epidemiological disease transmission modeling. V. cholerae genetics and the activity of virulence genes determine the pathogenic potential of different strains, as well as provide a model for genomic evolution in the natural environment. While animal models for V. cholerae infection have been used for decades, recent advances in this area provide a well-rounded picture of nearly all aspects of V. cholerae interaction with both mammalian and non-mammalian hosts, encompassing colonization dynamics, pathogenesis, immunological responses, and transmission to naïve populations. Microbiome studies have become increasingly common as access and affordability of sequencing has improved, and these studies have revealed key factors in V. cholerae communication and competition with members of the gut microbiota. Despite a wealth of knowledge surrounding V. cholerae, the pathogen remains endemic in numerous countries and causes sporadic outbreaks elsewhere. Public health initiatives aim to prevent cholera outbreaks and provide prompt, effective relief in cases where prevention is not feasible. In this review, we describe recent advancements in cholera research in these areas to provide a more complete illustration of V. cholerae evolution as a microbe and significant global health threat, as well as how researchers are working to improve understanding and minimize impact of this pathogen on vulnerable populations.
Mass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids
Authors: Cappuccio Gerarda , Khalil Saleh M. , Osenberg Sivan , Li Feng , Maletic-Savatic Mirjana
Published: May 2023

Human brain organoids are emerging models to study human brain development and pathology as they recapitulate the development and characteristics of major neural cell types, and enable manipulation through an in vitro system. Over the past decade, with the advent of spatial technologies, mass spectrometry imaging (MSI) has become a prominent tool for metabolic microscopy, providing label-free, non-targeted molecular and spatial distribution information of the metabolites within tissue, including lipids. This technology has never been used for studies of brain organoids and here, we set out to develop a standardized protocol for preparation and mass spectrometry imaging of human brain organoids. We present an optimized and validated sample preparation protocol, including sample fixation, optimal embedding solution, homogenous deposition of matrices, data acquisition and processing to maximize the molecular information derived from mass spectrometry imaging. We focus on lipids in organoids, as they play critical roles during cellular and brain development. Using high spatial and mass resolution in positive- and negative-ion modes, we detected 260 lipids in the organoids. Seven of them were uniquely localized within the neurogenic niches or rosettes as confirmed by histology, suggesting their importance for neuroprogenitor proliferation. We observed a particularly striking distribution of ceramide-phosphoethanolamine CerPE 36:1; O2 which was restricted within rosettes and of phosphatidyl-ethanolamine PE 38:3, which was distributed throughout the organoid tissue but not in rosettes. This suggests that ceramide in this particular lipid species might be important for neuroprogenitor biology, while its removal may be important for terminal differentiation of their progeny. Overall, our study establishes the first optimized experimental pipeline and data processing strategy for mass spectrometry imaging of human brain organoids, allowing direct comparison of lipid signal intensities and distributions in these tissues. Further, our data shed new light on the complex processes that govern brain development by identifying specific lipid signatures that may play a role in cell fate trajectories. Mass spectrometry imaging thus has great potential in advancing our understanding of early brain development as well as disease modeling and drug discovery.
Slow ion concentration oscillations and multiple states in neuron–glia interaction—insights gained from reduced mathematical models
Authors: Øyehaug Leiv
Published: May 2023

When potassium in the extracellular space separating neurons and glia reaches sufficient levels, neurons may fire spontaneous action potentials or even become inactivated due to membrane depolarisation, which, in turn, may lead to increased extracellular potassium levels. Under certain circumstances, this chain of events may trigger periodic bursts of neuronal activity. In the present study, reduced neuron–glia models are applied to explore the relationship between bursting behaviour and ion concentration dynamics. These reduced models are built based on a previously developed neuron–glia model, in which channel-mediated neuronal sodium and potassium currents are replaced by a function of neuronal sodium and extracellular potassium concentrations. Simulated dynamics of the resulting two reduced models display features that are qualitatively similar to those of the existing neuron–glia model. Bifurcation analyses of the reduced models show rich and interesting dynamics that include the existence of Hopf bifurcations between which the models exhibit slow ion concentration oscillations for a wide range of parameter values. The study demonstrates that even very simple models can provide insights of possible relevance to complex phenomena.
Altered flexor carpi radialis motor axon excitability properties after cerebrovascular stroke
Authors: Klein C.S. , Liu H. , Zhao C. , Huang W.
Published: May 2023

Background

Spinal motoneurons may become hyperexcitable after a stroke. Knowledge about motoneuron hyperexcitability remains clinically important as it may contribute to a number of phenomena including spasticity, flexion synergies, and abnormal limb postures. Hyperexcitability seems to occur more often in muscles that flex the wrist and fingers (forearm flexors) compared to other upper limb muscles. The cause of hyperexcitability remains uncertain but may involve plastic changes in motoneurons and their axons.

Aim

To characterize intrinsic membrane properties of flexor carpi radialis (FCR) motor axons after stroke using nerve excitability testing.

Methods

Nerve excitability testing using threshold tracking techniques was applied to characterize FCR motor axon properties in persons who suffered a first-time unilateral cortical/subcortical stroke 23 to 308  days earlier. The median nerve was stimulated at the elbow bilaterally in 16 male stroke subjects (51.4 ± 2.9 y) with compound muscle action potentials recorded from the FCR. Nineteen age-matched males (52.7 ± 2.4 y) were also tested to serve as controls.

Results

Axon parameters after stroke were consistent with bilateral hyperpolarization of the resting potential. Nonparetic and paretic side axons were modeled by a 2.6-fold increase in pump currents (IPumpNI) together with an increase (38%–33%) in internodal leak conductance (GLkI) and a decrease (23%–29%) in internodal H conductance (Ih) relative to control axons. A decrease (14%) in Na+channel inactivation rate (Aah) was also needed to fit the paretic axon recovery cycle. “Fanning out” of threshold electrotonus and the resting I/V slope (stroke limbs combined) correlated with blood potassium [K+] (R= −0.61 to 0.62,p< 0.01) and disability (R= −0.58 to 0.55,p< 0.05), but not with spasticity, grip strength, or maximal FCR activity.

Conclusion

In contrast to our expectations, FCR axons were not hyperexcitable after stroke. Rather, FCR axons were found to be hyperpolarized bilaterally post stroke, and this was associated with disability and [K+]. Reduced FCR axon excitability may represent a kind of bilateral trans-synaptic homeostatic mechanism that acts to minimize motoneuron hyperexcitability.
INs and OUTs of faces in consciousness: a study of the temporal evolution of consciousness of faces during binocular rivalry
Authors: Quettier Thomas , Di Lello Nicolò , Tsuchiya Naotsugu , Sessa Paola
Published: May 2023

Contents of consciousness change over time. However, the study of dynamics in consciousness has been largely neglected. Aru and Bachmann have recently brought to the attention of scientists dealing with consciousness the relevance of making inquiries about its temporal evolution. Importantly, they also pointed out several experimental questions as guidelines for researchers interested in studying the temporal evolution of consciousness, including the phases of formation and dissolution of content. They also suggested that these two phases could be characterized by asymmetric inertia. The main objective of the present investigation was to approximate the dynamics of these two phases in the context of conscious face perception. To this aim, we tested the time course of content transitions during a binocular rivalry task using face stimuli and asked participants to map their subjective experience of transitions from one content to the other through a joystick. We then computed metrics of joystick velocity linked to content transitions as proxies of the formation and dissolution phases. We found a general phase effect such that the formation phase was slower than the dissolution phase. Furthermore, we observed an effect specific to happy facial expressions, such that their contents were slower to form and dissolve than that of neutral expressions. We further propose to include a third phase of stabilization of conscious content between formation and dissolution.
Identification of necroptosis-related genes in Parkinson’s disease by integrated bioinformatics analysis and experimental validation
Authors: Lei Cheng , Zhongyan Zhou , Wenting Shi , Jing Zhang , Liyun Qin , Hongyi Hu , Juntao Yan , Qing Ye
Published: May 2023

Background

Parkinson’s disease (PD) is the second most common neurodegeneration disease worldwide. Necroptosis, which is a new form of programmed cell death with high relationship with inflammation, plays a vital role in the progression of PD. However, the key necroptosis related genes in PD are not fully elucidated.

Purpose

Identification of key necroptosis-related genes in PD.

Method

The PD associated datasets and necroptosis related genes were downloaded from the GEO Database and GeneCards platform, respectively. The DEGs associated with necroptosis in PD were obtained by gap analysis, and followed by cluster analysis, enrichment analysis and WGCNA analysis. Moreover, the key necroptosis related genes were generated by PPI network analysis and their relationship by spearman correlation analysis. Immune infiltration analysis was used for explore the immune state of PD brain accompanied with the expression levels of these genes in various types of immune cells. Finally, the gene expression levels of these key necroptosis related genes were validated by an external dataset, blood samples from PD patients and toxin-induced PD cell model using real-time PCR analysis.

Result

Twelve key necroptosis-related genes including ASGR2, CCNA1, FGF10, FGF19, HJURP, NTF3, OIP5, RRM2, SLC22A1, SLC28A3, WNT1 and WNT10B were identified by integrated bioinformatics analysis of PD related dataset GSE7621. According to the correlation analysis of these genes, RRM2 and WNT1 were positively and negatively correlated with SLC22A1 respectively, while WNT10B was positively correlated with both OIF5 and FGF19. As the results from immune infiltration analysis, M2 macrophage was the highest population of immune cell in analyzed PD brain samples. Moreover, we found that 3 genes (CCNA1, OIP5 and WNT10B) and 9 genes (ASGR2, FGF10, FGF19, HJURP, NTF3, RRM2, SLC22A1, SLC28A3 and WNT1) were down- and up- regulated in an external dataset GSE20141, respectively. All the mRNA expression levels of these 12 genes were obviously upregulated in 6-OHDA-induced SH-SY5Y cell PD model while CCNA1 and OIP5 were up- and down- regulated, respectively, in peripheral blood lymphocytes of PD patients.

Conclusion

Necroptosis and its associated inflammation play fundamental roles in the progression of PD and these identified 12 key genes might be served as new diagnostic markers and therapeutic targets for PD.
Altered GABAergic, glutamatergic and endocannabinoid signaling is accompanied by neuroinflammatory response in a zebrafish model of social withdrawal behavior
Authors: Perdikaris Panagiotis , Dermon Catherine R.
Published: May 2023

Introduction

Deficits in social communication are in the core of clinical symptoms characterizing many neuropsychiatric disorders such as schizophrenia and autism spectrum disorder. The occurrence of anxiety-related behavior, a common co-morbid condition in individuals with impairments in social domain, suggests the presence of overlapping neurobiological mechanisms between these two pathologies. Dysregulated excitation/inhibition balance and excessive neuroinflammation, in specific neural circuits, are proposed as common etiological mechanisms implicated in both pathologies.

Methods and Results

In the present study we evaluated changes in glutamatergic/GABAergic neurotransmission as well as the presence of neuroinflammation within the regions of the Social Decision-Making Network (SDMN) using a zebrafish model of NMDA receptor hypofunction, following sub-chronic MK-801 administration. MK-801-treated zebrafish are characterized by impaired social communication together with increased anxiety levels. At the molecular level, the behavioral phenotype was accompanied by increased mGluR5 and GAD67 but decreased PSD-95 protein expression levels in telencephalon and midbrain. In parallel, MK-801-treated zebrafish exhibited altered endocannabinoid signaling as indicated by the upregulation of cannabinoid receptor 1 (CB1R) in the telencephalon. Interestingly, glutamatergic dysfunction was positively correlated with social withdrawal behavior whereas defective GABAergic and endocannabinoid activity were positively associated with anxiety-like behavior. Moreover, neuronal and astrocytic IL-1β expression was increased in regions of the SDMN, supporting the role of neuroinflammatory responses in the manifestation of MK-801 behavioral phenotype. Colocalization of interleukin-1β (IL-1β) with β2-adrenergic receptors (β2-ARs) underlies the possible influence of noradrenergic neurotransmission to increased IL-1β expression in comorbidity between social deficits and elevated anxiety comorbidity.

Discussion

Overall, our results indicate the contribution of altered excitatory and inhibitory synaptic transmission as well as excessive neuroinflammatory responses in the manifestation of social deficits and anxiety-like behavior of MK-801-treated fish, identifying possible novel targets for amelioration of these symptoms.
Laparoscopic versus ultrasound-guided transversus abdominis plane block for postoperative pain management in minimally invasive colorectal surgery: a meta-analysis protocol
Authors: Yang Wenming , Yuan Tao , Cai Zhaolun , Ma Qin , Liu Xueting , Zhou Hang , Qiu Siyuan , Yang Lie
Published: May 2023

Introduction

Transversus abdominis plane block (TAPB) is now commonly administered for postoperative pain control and reduced opioid consumption in patients undergoing major colorectal surgeries, such as colorectal cancer, diverticular disease, and inflammatory bowel disease resection. However, there remain several controversies about the effectiveness and safety of laparoscopic TAPB compared to ultrasound-guided TAPB. Therefore, the aim of this study is to integrate both direct and indirect comparisons to identify a more effective and safer TAPB approach.

Materials and methods

Systematic electronic literature surveillance will be performed in the PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), and ClinicalTrials.gov databases for eligible studies through July 31, 2023. The Cochrane Risk of Bias version 2 (RoB 2) and Risk of Bias in Non-randomized Studies of Interventions (ROBINS-I) tools will be applied to scrutinize the methodological quality of the selected studies. The primary outcomes will include (1) opioid consumption at 24 hours postoperatively and (2) pain scores at 24 hours postoperatively both at rest and at coughing and movement according to the numerical rating scale (NRS). Additionally, the probability of TAPB-related adverse events, overall postoperative 30-day complications, postoperative 30-day ileus, postoperative 30-day surgical site infection, postoperative 7-day nausea and vomiting, and length of stay will be analyzed as secondary outcome measures. The findings will be assessed for robustness through subgroup analyses and sensitivity analyses. Data analyses will be performed using RevMan 5.4.1 and Stata 17.0. P value of less than 0.05 will be defined as statistically significant. The certainty of evidence will be examined via the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) working group approach.

Ethics and dissemination

Owing to the nature of the secondary analysis of existing data, no ethical approval will be required. Our meta-analysis will summarize all the available evidence for the effectiveness and safety of TAPB approaches for minimally invasive colorectal surgery. High-quality peer-reviewed publications and presentations at international conferences will facilitate disseminating the results of this study, which are expected to inform future clinical trials and help anesthesiologists and surgeons determine the optimal tailored clinical practice for perioperative pain management.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=281720, identifier (CRD42021281720).
Effects of paddles and fins on front crawl kinematics, arm stroke efficiency, coordination, and estimated energy cost
Authors: De Matos C. C. , Guignard B. , Castro F. De Souza , Guimard A.
Published: May 2023

Paddles and fins are used in swimmers training with different objectives (e.g., increase propulsive areas of hands and feet, improve the feeling of water flow). These artificial modifications of the stroke might be viewed as external constraints of the stroke task, both will either disturb or facilitate swimming modalities, so the coaches should manipulate its use to extract benefits for performance. This study seeks to investigate the precise effects of wearing either paddles (PAD) or fins (FINS) vs. a no-equipment (NE) trial in three all-out front crawl exercises on swimmer kinematics, arm stroke efficiency (ηp), upper-limbs coordination patterns (Index of Coordination, IdC), and estimated energy cost (C). Eleven regional to national-level male swimmers participated in the study (age: 25.8 ± 5.5 years, body mass: 75.2 ± 5.5 kg, height: 177 ± 6.5 cm) and were recorded from both sides of the swimming pool to collect all variables. Repeated measures ANOVA and Bonferroni post hoc were used to compare the variables. Effects sizes were calculated. Time to cover the distance and velocity were higher in FINS swimming, with larger values of stroke length (SL) and lower kick amplitude in comparison to the other trials (PAD and NE). The use of FINS also modified the stroke phases durations by presenting significant lower propulsion time during the stroke in comparison to PAD or NE. Values of IdC were lower (IdC < −1%, so catch-up pattern of coordination) for FINS in comparison to NE. In terms of ηp, using PAD or FINS demonstrate higher arm stroke efficiency than swimming without equipment. Finally, C was significantly higher in FINS swimming in comparison to NE and PAD. From the present results, it should be noted that the use of equipment such as fins deeply modify the structure of the swimming stroke (from the performance-related parameters through the kinematics of both upper and lower limbs to the stroke efficiency and coordination pattern). So, using equipment should be appropriately scaled by the coaches to the objectives of the training session in swimming, and in emergent sports such as “SwimRun”, paddles and fins must be viewed as tools to achieve higher velocities to cover a given distance.
Transcriptome analysis of the gene expression of M. iliotibialis lateralis affected by dietary methionine restriction
Authors: Li Desheng , Chen Fei , Tian Yumin , Su Yuhong
Published: May 2023

Introduction: Methionine (Met) is an important amino acid related to the development of skeletal muscle. This study investigated the effects of dietary Met restriction on the gene expression of M. iliotibialis lateralis.

Methods: A total of 84 day-old broiler chicks (Zhuanghe Dagu) with a similar initial body weight (207.62 ± 8.54 g) were used in this study. All birds were divided into two groups (CON; L-Met) based on the initial body weight. Each group consisted of six replicates with seven birds per replicate. The experimental period was 63 days (phase 1, days 1-21; phase 2, days 22-63). According to the nutritional requirements of Zhuanghe Dagu chickens, we provided a basal diet (0.39% Met levels during phase 1 and 0.35% Met levels during phase 2, as-fed basis) to the birds in the CON group, while we provided a Met-restricted diet (0.31% Met levels during phase 1 and 0.28% Met levels during phase 2, as-fed basis) to the birds in the L-Met group. The growth performance of broiler chicks and their M. iliotibialis lateralis development parameters were measured on days 21 and 63.

Results and Discussion: In this study, dietary Met restriction did not affect the growth performance of broiler chicks but hindered the development of M. iliotibialis lateralis at both sampling timepoints. On the final day, three birds selected from each group (three from CON and three from L-Met) were used to obtain M. iliotibialis lateralis samples from leg muscle for further transcriptome analysis. Transcriptome analysis revealed that dietary Met restriction significantly upregulated 247 differentially expressed genes (DEGs) and downregulated 173 DEGs. Additionally, DEGs were mainly enriched in 10 pathways. Among DEGs, we observed that dietary Met restriction downregulated the expression of CSRP3, KY, FHL1, LMCD1, and MYOZ2 in M. iliotibialis lateralis. Therefore, we considered that dietary Met restriction had negative effects on the development of M. iliotibialis lateralis, and CSRP3, KY, FHL1, LMCD1, and MYOZ2 may serve as potential functional genes involved in this process.
Exposure to continuous political violence: rational and experiential thinking styles, coping styles and post traumatic stress symptoms
Authors: Zvi Liza , Cohen-Louck Keren
Published: May 2023

Cognitive style is considered an important determinant of individual behavior. The aim of the present study was to examine the relations between rational and experiential thinking styles, coping styles and Post Traumatic Stress (PTS) symptoms among civilians exposed to continuous and ongoing exposure to political violence. Three-hundred and thirty-two Israeli adult citizens living in the south region of Israel reported on their experiences of exposure to political violence as well as level of PTS, coping styles, and preference toward rational and experiential processing style. Results showed that low rational thinking was related with elevated PTS, both directly and indirectly through the mediation of high emotion-focused coping. The findings suggest that rational thinking may serve as a protective factor against stress related to chronic exposure to political violence; conversely, a preference for low rationality may be a risk factor.
Perceived teacher-student relationship and growth mindset as predictors of student engagement in foreign student engagement in foreign language learning: the mediating role of foreign language enjoyment
Authors: Li Haoting
Published: May 2023

Introduction

This study aimed to investigate the relationships between perceived teacher-student relationship, growth mindset, student engagement, and foreign language enjoyment (FLE) among Chinese English learners.

Methods

A total of 413 Chinese EFL learners participated in the study and completed self-report measures for perceived teacher-student relationship, growth mindset, student engagement in foreign language learning, and FLE. Confirmatory factor analysis was employed to assess the validity of the scales. Structural equation modeling was used to test the hypothesized model.

Results

The partial mediation model demonstrated the best fit to the data. The results indicated that perceived teacher-student relationship had a direct impact on student engagement. FLE directly influenced student engagement, while growth mindset indirectly affected student engagement through the mediation of FLE.

Discussion

The findings suggest that fostering positive teacher-student relationships and promoting a growth mindset can enhance FLE, leading to increased levels of student engagement. These results emphasize the importance of considering both the interpersonal dynamics between teachers and students and the role of mindset in foreign language learning.
The expansion of vanity of Simon in A Portrait of the Artist as a Young Man
Authors: Farahmandian Hamid , Liu Chaojian
Published: May 2023

An aggressive personality is a distorted personality with dark traits such as arrogance, a sense of power over others, and exploitation. As per Karen Horney's theory of neurosis, all these traits make an individual, psychologically, neurotic, who is willing to go against other people in society. In this paper, drawing upon Horney's theory, I study the aggressive personality of Simon in James Joyce's A Portrait of the Artist as a Young Man from three aspects of frustrated selfishness, sense of dominance, and search for respect to make known his neurotic needs for power, admiration, prestige, exploitation and achievement and demonstrate that offensive conducts of Simon make him more insecure and leave him more aggressive toward everybody at home and society.
COVID-19 government measures and their impact on mental health: a cross-sectional study of older primary care patients in Germany
Authors: Wittmann Felix G. , Zülke Andrea , Pabst Alexander , Luppa Melanie , Thyrian Jochen René , Kästner Anika , Hoffmann Wolfgang , Kaduszkiewicz Hanna , Döhring Juliane , Escales Catharina , Gensichen Jochen , Zöllinger Isabel , Kosilek Robert Philipp , Wiese Birgitt , Oey Anke , König Hans-Helmut , Brettschneider Christian , Frese Thomas , Riedel-Heller Steffi G.
Published: May 2023

Background

With the outbreak of COVID-19, government measures including social distancing and restrictions of social contacts were imposed to slow the spread of the virus. Since older adults are at increased risk of severe disease, they were particularly affected by these restrictions. These may negatively affect mental health by loneliness and social isolation, which constitute risk factors for depressiveness. We aimed to analyse the impact of perceived restriction due to government measures on depressive symptoms and investigated stress as mediator in an at-risk-population in Germany.

Methods

Data were collected in April 2020 from the population of theAgeWell.de-study, including individuals with a Cardiovascular Risk Factors, Aging, and Incidence of Dementia (CAIDE) score ≥9, using the depression subscale of the Brief Symptom Inventory (BSI-18) and the Perceived Stress Scale (PSS-4). Feeling restricted due to COVID-19 government measures was surveyed with a standardized questionnaire. Stepwise multivariate regressions using zero-inflated negative binomial models were applied to analyse depressive symptoms, followed by a general structural equation model to assess stress as mediator. Analysis were controlled for sociodemographic factors as well as social support.

Results

We analysed data from 810 older adults (mean age = 69.9, SD = 5). Feeling restricted due to COVID-19 government measures was linked to increased depressiveness (b = 0.19;p < 0.001). The association was no longer significant when adding stress and covariates (b = 0.04;p = 0.43), while stress was linked to increased depressive symptoms (b = 0.22;p < 0.001). A final model confirms the assumption that the feeling of restriction is mediated by stress (total effect:b = 0.26;p < 0.001).

Conclusion

We found evidence that feeling restricted due to COVID-19 government measures is associated with higher levels of depressive symptoms in older adults at increased risk for dementia. The association is mediated by perceived stress. Furthermore, social support was significantly associated with less depressive symptoms. Thus, it is of high relevance to consider possible adverse effects of government measures related to COVID-19 on mental health of older people.
Neglected burden of injuries in Ethiopia, from 1990 to 2019: a systematic analysis of the global burden of diseases study 2019
Authors: Berheto Tezera Moshago , Sengoelge Mathilde , Tadesse Sebsibe , Workie Shimelash Bitew , Tessema Gizachew , Memirie Solomon Tessema , Mohammed Shikur , Getnet Fentabil , Walker Ally , Naghavi Mohsen , Misganaw Awoke
Published: May 2023

Background

The 2030 agenda for sustainable development goals has given injury prevention new attention, including halving road traffic injuries. This study compiled the best available evidence on injury from the global burden of diseases study for Ethiopia from 1990 to 2019.

Methods

Injury data on incidence, prevalence, mortality, disability-adjusted life years lost, years lived with disability, and years of life lost were extracted from the 2019 global burden of diseases study for regions and chartered cities in Ethiopia from 1990 to 2019. Rates were estimated per 100,000 population.

Results

In 2019, the age-standardized rate of incidence was 7,118 (95% UI: 6,621–7,678), prevalence was 21,735 (95% UI: 19,251–26,302), death was 72 (95% UI: 61–83), disability-adjusted life years lost was 3,265 (95% UI: 2,826–3,783), years of live lost was 2,417 (95% UI: 2,043–2,860), and years lived with disability was 848 [95% UI: (620–1,153)]. Since 1990, there has been a reduction in the age-standardized rate of incidence by 76% (95% UI: 74–78), death by 70% (95% UI: 65–75), and prevalence by 13% (95% UI: 3–18), with noticeable inter-regional variations. Transport injuries, conflict and terrorism, interpersonal violence, self-harm, falls, poisoning, and exposure to mechanical forces were the leading causes of injury-related deaths and long-term disabilities. Since 1990, there has been a decline in the prevalence of transport injuries by 32% (95% UI: 31–33), exposure to mechanical forces by 12% (95% UI: 10–14), and interpersonal violence by 7.4% (95% UI: 5–10). However, there was an increment in falls by 8.4% (95% UI: 7–11) and conflict and terrorism by 1.5% (95% UI: 38–27).

Conclusion

Even though the burden of injuries has steadily decreased at national and sub-national levels in Ethiopia over the past 30 years, it still remains to be an area of public health priority. Therefore, injury prevention and control strategies should consider regional disparities in the burden of injuries, promoting transportation safety, developing democratic culture and negotiation skills to solve disputes, using early security-interventions when conflict arises, ensuring workplace safety and improving psychological wellbeing of citizens.
A case study for unlocking the potential of deep learning in asset-liability-management
Authors: Krabichler Thomas , Teichmann Josef
Published: May 2023

The extensive application of deep learning in the field of quantitative risk management is still a relatively recent phenomenon. This article presents the key notions of Deep Asset-Liability-Management (“Deep ALM”) for a technological transformation in the management of assets and liabilities along a whole term structure. The approach has a profound impact on a wide range of applications such as optimal decision making for treasurers, optimal procurement of commodities or the optimization of hydroelectric power plants. As a by-product, intriguing aspects of goal-based investing or Asset-Liability-Management (ALM) in abstract terms concerning urgent challenges of our society are expected alongside. We illustrate the potential of the approach in a stylized case.
6-hour Training in click-based echolocation changes practice in visual impairment professionals
Authors: Thaler Lore , Di Gregorio Giammarco , Foresteire Denise
Published: May 2023

Click-based echolocation can support mobility and orientation in people with vision impairments (VI) when used alongside other mobility methods. Only a small number of people with VI use click-based echolocation. Previous research about echolocation addresses the skill of echolocation per se to understand how echolocation works, and its brain basis. Our report is the first to address the question of professional practice for people with VI, i.e., a very different focus. VI professionals are well placed to affect how a person with VI might learn about, experience or use click-based echolocation. Thus, we here investigated if training in click-based echolocation for VI professionals might lead to a change in their professional practice. The training was delivered via 6-h workshops throughout the UK. It was free to attend, and people signed up via a publicly available website. We received follow-up feedback in the form of yes/no answers and free text comments. Yes/no answers showed that 98% of participants had changed their professional practice as a consequence of the training. Free text responses were analysed using content analysis, and we found that 32%, 11.7% and 46.6% of responses indicated a change in information processing, verbal influencing or instruction and practice, respectively. This attests to the potential of VI professionals to act as multipliers of training in click-based echolocation with the potential to improve the lives of people with VI. The training we evaluated here could feasibly be integrated into VI Rehabilitation or VI Habilitation training as implemented at higher education institutions (HEIs) or continuing professional development (CPD).
RCM modeling of bubble injections into the inner magnetosphere: geosynchronous orbit and the ionospheric responses
Authors: Sadeghzadeh Sina , Yang Jian , Toffoletto Frank , Wolf Richard , Mousavi Ameneh , Wang Chih-Ping
Published: May 2023

Introduction: Accurate characterization of the plasma sheet source population in the ring current region and its outer boundary at geosynchronous orbit is crucial for understanding the dynamics of the Earth’s magnetosphere. The interaction between the ring current and plasma populations from the ionosphere is a focus of extensive research.

Methods: We used the Rice Convection Model (RCM) to simulate the transient meso-scale injections of fast flows or plasma sheet bubbles from the outer boundary into the inner magnetosphere and the associated impacts on the ionosphere. We compared our simulation results of the average properties of bulk plasma access to geosynchronous orbit to a number of empirical models. We also examined the role of plasma sheet bubbles in forming field-aligned currents (FACs).

Results: Our modeling results show that impulsive plasma sheet injections dramatically alter the average distribution of FACs in the ionosphere. We found both quantitative and qualitative agreements and disagreements when comparing our simulation results to empirical models. Furthermore, we demonstrated that several discrete auroral structures can be identified in the nightside ionosphere in accordance with theupward FACs.

Discussion: The significance of plasma sheet bubbles in modifying the averageplasma properties at geosynchronous orbit and FACs in the ionosphere is highlighted by oursimulation findings, offering novel understandings into the dynamics of Earth's magnetosphere,and emphasizing the necessity for further research in this field.
Factors Associated With COVID-19 Non-Vaccination in Switzerland: A Nationwide Study
Authors: Sabatini Serena , Kaufmann Marco , Fadda Marta , Tancredi Stefano , Noor Nazihah , Van Der Linden Bernadette W. A. , Cullati Stéphane , Frank Irene , Michel Gisela , Harju Erika , Luedi Chantal , Frei Anja , Ballouz Tala , Menges Dominik , Fehr Jan , Kohler Philipp , Kahlert Christian R. , Scheu Victor , Ortega Natalia , Chocano-Bedoya Patricia , Rodondi Nicolas , Stringhini Silvia , Baysson Hélène , Lorthe Elsa , Zufferey Maria Caiata , Suggs L. Suzanne , Albanese Emiliano , Vincentini Julia , Bochud Murielle , D’Acremont Valérie , Nusslé Samira Gonseth , Imboden Medea , Keidel Dirk , Witzig Melissa , Probst-Hensch Nicole , von Wyl Viktor
Published: May 2023

Objectives: We compared socio-demographic characteristics, health-related variables, vaccination-related beliefs and attitudes, vaccination acceptance, and personality traits of individuals who vaccinated against COVID-19 and who did not vaccinate by December 2021.

Methods: This cross-sectional study used data of 10,642 adult participants from the Corona Immunitas eCohort, an age-stratified random sample of the population of several cantons in Switzerland. We used multivariable logistic regression models to explore associations of vaccination status with socio-demographic, health, and behavioral factors.

Results: Non-vaccinated individuals represented 12.4% of the sample. Compared to vaccinated individuals, non-vaccinated individuals were more likely to be younger, healthier, employed, have lower income, not worried about their health, have previously tested positive for SARS-CoV-2 infection, express lower vaccination acceptance, and/or report higher conscientiousness. Among non-vaccinated individuals, 19.9% and 21.3% had low confidence in the safety and effectiveness of SARS-CoV-2 vaccine, respectively. However, 29.1% and 26.7% of individuals with concerns about vaccine effectiveness and side effects at baseline, respectively vaccinated during the study period.

Conclusion: In addition to known socio-demographic and health-related factors, non-vaccination was associated with concerns regarding vaccine safety and effectiveness.
Od dezintegracji do integracji społecznej. Praktyki repartycji jako polityczne narzędzia przekształcania społeczeństwa
Authors: Borowiec Piotr
Published: May 2023

The article discusses the significance of the practices of repartition for the disintegration and integration of social groups. It has been emphasized that repartitions are not only generated by social expectations, mobilise the voters, and help politicians take advantage of social problems, but they also contribute to the integration of social groups. The groups comprised of the supporters of repartitions, who accept their message, and also of their opponents, who irrevocably reject the changes put forward by the repartitions. The power of repartitions, which, first and foremost, resides in the legality of the system, the refusal to accepts its inequalities and in the promise of new fair hierarchies translates into the creation of social bonds. Repartitions bring together those who are excluded and want for goods with those who have them in excess. By bringing the individuals together, repartitions make the creation of a fair order more likely. It is because of these properties that they become politically useful tools, instrumental in remodelling the society, which ultimately leads to its integration into one entity.