bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical Activity Participation and the Environment in Children and Adolescents: A Systematic Review and Meta-Analysis Protocol
Authors: Li Longxi , Moosbrugger Michelle E. , Liu Yang
Published: June 2021

Physical activity (PA) and sports are efficient ways to promote the younger generation’s health and wellbeing. However, evidence is limited due to heterogeneous samples and measurements. This study aims to identify promoting and inhibiting correlates associated with children’s and adolescents’ non-organized PA participation and further demonstrate the complexity of PA and ecological factors. A systematic review and meta-analysis will be applied by following the preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P). Seven bibliographic databases (PubMed, SPORTDiscus, PsycInfo, MEDLINE Complete, ERIC, Dimensions, and Academic Search Complete) will be systematically searched to identify eligible articles based on a series of inclusion and exclusion criteria. Inclusion criteria are that the study: (a) is not classified as a systematic review with or without meta-analysis; (b) is published in last 20 years; (c) includes children and adolescents; (d) quantitively measures PA; (e) includes review of ecological factors. The internal validity will be evaluated using a validated quality instrument. Calculations will be produced in SPSS 27.0 and Comprehensive Meta-Analysis 3.3. This study will provide evidence and address the questions regarding the factors that significantly impact children’s PA participation and limitations regarding the design, sampling, and measurement in currently selected studies. PROSPERO registration number: CRD42021244918.
Association between Shift Work and Reflux Esophagitis: The Kangbuk Samsung Health Study
Authors: Mun Eunchan , Kim Daehoon , Lee Yesung , Lee Woncheol , Park Soyoung
Published: June 2021

This cross-sectional study investigated the relationship between shift work and reflux esophagitis verified by endoscopic findings. Participants underwent a comprehensive health examination and esophagogastroduodenoscopy between January 2011 and December 2018. We examined endoscopic findings and performed multivariate-adjusted regression analysis of the association between shift work and reflux esophagitis using multiple demographic and clinical factors. Among the 247,450 participants, 49,767 (20.1%) had reflux esophagitis; Los Angeles (LA)-M (31,132, 12.6%) was most common, followed by LA-A (16,213, 6.6%), LA-B (2333, 0.9%), and ≥LA-C (89, 0.04%). The multivariate-fully adjusted odds ratio (OR) of overall reflux esophagitis for shift work compared to fixed day work was 1.15 (95% confidence interval [CI]: 1.11–1.19). When classified according to shift work type and severity of reflux esophagitis, the ORs of LA-A for regular day and night, and irregular shifts compared to fixed day works were 1.14 (95% CI: 1.03–1.26) and 1.26 (95% CI: 1.11–1.44), respectively. However, there was no significant association between any shift work schedule and ≥LA-B. Overall, we demonstrated the cross-sectional association between shift work (especially rotating and irregular shifts) and mild reflux esophagitis (≤LA-A) compared with daily fixed time shifts.
Lagged Association between Climate Variables and Hospital Admissions for Pneumonia in South Africa
Authors: Pedder Hugo , Kapwata Thandi , Howard Guy , Naidoo Rajen N. , Kunene Zamantimande , Morris Richard W. , Mathee Angela , Wright Caradee Y.
Published: June 2021

Pneumonia is a leading cause of hospitalization in South Africa. Climate change could potentially affect its incidence via changes in meteorological conditions. We investigated the delayed effects of temperature and relative humidity on pneumonia hospital admissions at two large public hospitals in Limpopo province, South Africa. Using 4062 pneumonia hospital admission records from 2007 to 2015, a time-varying distributed lag non-linear model was used to estimate temperature-lag and relative humidity-lag pneumonia relationships. Mean temperature, relative humidity and diurnal temperature range were all significantly associated with pneumonia admissions. Cumulatively across the 21-day period, higher mean daily temperature (30 °C relative to 21 °C) was most strongly associated with a decreased rate of hospital admissions (relative rate ratios (RR): 0.34, 95% confidence interval (CI): 0.14–0.82), whereas results were suggestive of lower mean daily temperature (12 °C relative to 21 °C) being associated with an increased rate of admissions (RR: 1.27, 95%CI: 0.75–2.16). Higher relative humidity (>80%) was associated with fewer hospital admissions while low relative humidity (<30%) was associated with increased admissions. A proportion of pneumonia admissions were attributable to changes in meteorological variables, and our results indicate that even small shifts in their distributions (e.g., due to climate change) could lead to substantial changes in their burden. These findings can inform a better understanding of the health implications of climate change and the burden of hospital admissions for pneumonia now and in the future.
Cost-Effectiveness Analysis of Dabrafenib Plus Trametinib and Vemurafenib as First-Line Treatment in Patients with BRAF V600 Mutation-Positive Unresectable or Metastatic Melanoma in China
Authors: Gao Tianfu , Liu Jia , Wu Jing
Published: June 2021

Objective: To evaluate the cost-effectiveness of dabrafenib plus trametinib combination therapy versus vemurafenib as first-line treatment in patients with BRAF V600 mutation-positive unresectable or metastatic melanoma from a healthcare system perspective in China. Methods: This study employed a partitioned survival model with three health states (progression-free survival, post-progression survival and dead) to parameterize the data derived from Combi-v trial and extrapolated to 30 years. Health states’ utilities were measured by EQ-5D-3L, also sourced from the Combi-v trial. Costs including drug acquisition costs, disease management costs and adverse event costs were based on the Chinese Drug Bidding Database and physician survey in China. The primary outcomes of the model were lifetime costs, life-years (LYs), quality-adjusted life-years (QALYs) and incremental cost-effectiveness ratio (ICER). Deterministic and probabilistic sensitivity analyses were conducted, respectively. Result: Dabrafenib plus trametinib is projected to increase a patient’s life expectancy by 0.95 life-years over vemurafenib (3.03 vs. 2.08) and 1.09 QALY gains (2.48 vs. 1.39) with an incremental cost of $3833. The incremental cost-effectiveness ratio (ICER) was $3511 per QALY. In the probabilistic sensitivity analyses, at a threshold of $33,357 per QALY (three times the gross domestic product (GDP) per capita in China in 2020), the probability of dabrafenib plus trametinib being cost-effective was 90%. In the deterministic sensitivity analyses, the results were most sensitive to the dabrafenib plus trametinib drug costs, vemurafenib drug costs and discount rate of cost. Conclusion: Dabrafenib plus trametinib therapy yields more clinical benefits than vemurafenib. Using a threshold of $33,357 per QALY, dabrafenib plus trametinib is very cost-effective as compared with vemurafenib in China.
A New Method of Removing Fine Particulates Using an Electrostatic Force
Authors: Heo Jaeseok , Jang Yelim , Versoza Michael , Kim Gihwan , Park Duckshin
Published: June 2021

Many studies have found that the concentration of fine particulates in the atmosphere has increased. In particular, when using the bus, the situation in which people are exposed to relatively high concentrations of fine particulates is increasing. The purpose of this study is to reduce exposure to these potentially harmful particulates by introducing open shelters at outdoor bus stops. In order to use it as an outdoor fine particulates reduction device, a brush filter using electrostatic force (EF) was used on an experimental scale and the generation of electrostatic force, according to the material, was examined. As electrostatic force was generated, the fine particulates collection performance was about 90% efficiency. In addition, it was confirmed that the efficiency of each particle size was improved by 57% through structural improvement. Finally, through experimentation, it was confirmed that the brush module can be used for about 70 days.
Application of a Scorecard Tool for Assessing and Engaging Media on Responsible Reporting of Suicide-Related News in India
Authors: Vijayakumar Lakshmi , Shastri Manisha , Fernandes Tanya Nicole , Bagra Yash , Pathare Aaryaman , Patel Arpita , Jain Padam , Merchant Yesha , Armstrong Gregory , Pathare Soumitra
Published: June 2021

Background: Each year there are more than 800,000 deaths by suicide across the world, while India alone accounts for one third of female suicides and one fourth of male suicides worldwide. Responsible media reporting of suicide is an important suicide prevention intervention at the population level. There is sufficient evidence to show that the way suicide is reported and portrayed in the media can have a significant impact on individuals experiencing suicidal thoughts and behaviors. Recognizing the important role of the media in suicide prevention, the World Health Organization (WHO) issued guidelines for responsible reporting of suicides by the media. The Press Council of India, in 2019 endorsed WHO’s guidelines for media reporting of suicides, however there is no evidence that the Indian media is complying with these guidelines. Methods: To encourage responsible media reporting, we developed a scorecard to assess and rate media reports on suicide. We reviewed several resource documents that contained guidelines on responsible reporting of suicide. After consulting with a team of experts, we arrived at a scorecard that consisted of 10 positive and 10 negative parameters. Results: We applied the scorecard to 1318 reports on suicide from 9 English language newspapers, with the highest readership in India between the dates of 1 April to 30 June 2020. For the articles analyzed, the average positive score across all newspapers was 1.32 and the average negative score was 3.31. Discussion: The scorecard can be a useful tool to assess media reports on suicide and provide metrics for the same. It can facilitate improved monitoring and engagement with media organizations, who can quickly check their own reporting compliance to the WHO guidelines and compare how well they are performing compared to their peers over time.
A Long-Term Incidence of Heart Failure and Predictors Following Newly Developed Acute Myocardial Infarction: A 10 Years Retrospective Cohort Study with Korean National Health Insurance Data
Authors: Choi Hyojung , Seo Joo Yeon , Shin Jinho , Choi Bo Youl , Kim Yu-Mi
Published: June 2021

Heart failure (HF) is the major mechanism of mortality in acute myocardial infarction (AMI) during early or intermediate post-AMI period. But heart failure is one of the most common long-term complications of AMI. Applied the retrospective cohort study design with nation representative population data, this study traced the incidence of late-onset heart failure since 1 year after newly developed acute myocardial infarction and assessed its risk factors. Methods and Results: Using the Korea National Health Insurance database, 18,328 newly developed AMI patients aged 40 years or older and first hospitalized in 2010 for 3 days or more, were set up as baseline cohort (12,403). The incidence rate of AMI per 100,000 persons was 79.8 overall, and 49.6 for women and 112.3 for men. A total of 2010 (1073 men, 937 women) were newly developed with HF during 6 years following post AMI. Cumulative incidences of HF per 1000 AMI patients for a year at each time period were 37.4 in initial hospitalization, 32.3 in 1 year after discharge, and 8.9 in 1–6 years. The overall and age-specific incidence rates of HF were higher in women than men. For late-onset HF, female, medical aid, pre-existing hypertension, severity of AMI, duration of hospital stay during index admission, reperfusion treatment, and drug prescription pattern including diuretics, affected the occurrence of late-onset HF. Conclusion: With respect to late-onset HF following AMI, appropriate management including hypertension and medical aid program in addition to quality improvement of AMI treatment are required to reduce the risk of late-onset heart failure.
Comparison of Body Scanner and Manual Anthropometric Measurements of Body Shape: A Systematic Review
Authors: Rumbo-Rodríguez Lorena , Sánchez-SanSegundo Miriam , Ferrer-Cascales Rosario , García-D’Urso Nahuel , Hurtado-Sánchez Jose A. , Zaragoza-Martí Ana
Published: June 2021

Anthropometrics are a set of direct quantitative measurements of the human body’s external dimensions, which can be used as indirect measures of body composition. Due to a number of limitations of conventional manual techniques for the collection of body measurements, advanced systems using three-dimensional (3D) scanners are currently being employed, despite being a relatively new technique. A systematic review was carried out using Pubmed, Medline and the Cochrane Library to assess whether 3D scanners offer reproducible, reliable and accurate data with respect to anthropometrics. Although significant differences were found, 3D measurements correlated strongly with measurements made by conventional anthropometry, dual-energy X-ray absorptiometry (DXA) and air displacement plethysmography (ADP), among others. In most studies (61.1%), 3D scanners were more accurate than these other techniques; in fact, these scanners presented excellent accuracy or reliability. 3D scanners allow automated, quick and easy measurements of different body tissues. Moreover, they seem to provide reproducible, reliable and accurate data that correlate well with the other techniques used.
Health Transparency and Communication on the Government Websites of Ibero-American Countries: The Cases of Chile, Colombia, Ecuador, and Spain
Authors: Barredo Ibáñez Daniel , Molina Rodríguez-Navas Pedro , Medranda Morales Narcisa Jessenia , Rodríguez Breijo Vanessa
Published: June 2021

Through the strategic use of health communication from their websites, government institutions can achieve greater promotion and prevent health issues for citizens, at whom such websites are aimed. Thus, the transparency of these sites is essential to favor issues such as participation, accountability, and good governance. In the present study, through content analysis, we examined how active transparency and communication in health is built through analyzing the information provided by the different administrative levels with competencies in this field (government, regions, and municipalities) from the following countries: Chile, Colombia, Ecuador, and Spain. The results were projected according to a double axis of analysis. On the one hand, we offer a description of the levels of management of this phenomenon (structural characteristics and available resources). On the other hand, we developed indices based on each of the countries to compare the typologies grouped in these Ibero-American countries. As a general conclusion, the results evinced insufficient transparency in common; however, the deficit was less in countries that had a public health system.
The Importance of Indigenous Cartography and Toponymy to Historical Land Tenure and Contributions to Euro/American/Canadian Cartography
Authors: Cole Daniel G. , Hart E. Richard
Published: June 2021

Indigenous maps are critical in understanding the historic and current land tenure of Indigenous groups. Furthermore, Indigenous claims to land can be seen in their connections via toponymy. European concepts of territory and political boundaries did not coincide with First Nation/American Indian views, resulting in the mistaken view that Natives did not have formal concepts of their territories. And Tribes/First Nations with cross-border territory have special jurisdictional problems. This paper illustrates how many Native residents were very spatially aware of their own lands, as well as neighboring nations’ lands, overlaps between groups, hunting territories, populations, and trade networks. Finally, the Sinixt First Nation serve as a perfect example of a case study on how an Aboriginal people are currently inputting and using a GIS representation of their territory with proper toponymy and use areas.
Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
Authors: Rosario-Colon Jonothan , Eberle Karen , Adams Abby , Courville Evan , Xin Hong
Published: June 2021

Candida auris is a multidrug-resistant fungal pathogen that can cause disseminated bloodstream infections with up to 60% mortality in susceptible populations. Of the three major classes of antifungal drugs, most C. auris isolates show high resistance to azoles and polyenes, with some clinical isolates showing resistance to all three drug classes. We reported in this study a novel approach to treating C. auris disseminated infections through passive transfer of monoclonal antibodies (mAbs) targeting cell surface antigens with high homology in medically important Candida species. Using an established A/J mouse model of disseminated infection that mimics human candidiasis, we showed that C3.1, a mAb that targets β-1,2-mannotriose (β-Man3), significantly extended survival and reduced fungal burdens in target organs, compared to control mice. We also demonstrated that two peptide-specific mAbs, 6H1 and 9F2, which target hyphal wall protein 1 (Hwp1) and phosphoglycerate kinase 1 (Pgk1), respectively, also provided significantly enhanced survival and reduction of fungal burdens. Finally, we showed that passive transfer of a 6H1+9F2 cocktail induced significantly enhanced protection, compared to treatment with either mAb individually. Our data demonstrate the utility of β-Man3- and peptide-specific mAbs as an effective alternative to antifungals against medically important Candida species including multidrug-resistant C. auris.
Roles of High Osmolarity Glycerol and Cell Wall Integrity Pathways in Cadmium Toxicity in Saccharomyces cerevisiae
Authors: Zhao Yunying , Li Shiyun , Wang Jing , Liu Yingli , Deng Yu
Published: June 2021

Cadmium is a carcinogen that can induce ER stress, DNA damage, oxidative stress and cell death. The yeast mitogen-activated protein kinase (MAPK) signalling pathways paly crucial roles in response to various stresses. Here, we demonstrate that the unfolded protein response (UPR) pathway, the high osmolarity glycerol (HOG) pathway and the cell wall integrity (CWI) pathway are all essential for yeast cells to defend against the cadmium-induced toxicity, including the elevated ROS and cell death levels induced by cadmium. We show that the UPR pathway is required for the cadmium-induced phosphorylation of HOG_MAPK Hog1 but not for CWI_MAPK Slt2, while Slt2 but not Hog1 is required for the activation of the UPR pathway through the transcription factors of Swi6 and Rlm1. Moreover, deletion of HAC1 and IRE1 could promote the nuclear accumulation of Hog1, and increase the cytosolic and bud neck localisation of Slt2, indicating crucial roles of Hog1 and Slt2 in regulating the cellular process in the absence of UPR pathway. Altogether, our findings highlight the significance of these two MAPK pathways of HOG and CWI and their interrelationship with the UPR pathway in responding to cadmium-induced toxicity in budding yeast.
Individual and Combined Treatments with Methylated Resveratrol Analogue DMU-214 and Gefitinib Inhibit Tongue Cancer Cells Growth via Apoptosis Induction and EGFR Inhibition
Authors: Jozkowiak Malgorzata , Dyszkiewicz-Konwinska Marta , Ramlau Piotr , Kranc Wieslawa , Spaczynska Julia , Wierzchowski Marcin , Kaczmarek Mariusz , Jodynis-Liebert Jadwiga , Piotrowska-Kempisty Hanna
Published: June 2021

The methylated resveratrol analogue 3′-hydroxy-3,4,5,4′-tetramethoxystilbene (DMU-214) has been revealed to exert the anti-cancer activity by a block of the cell cycle at the G2/M phase, apoptosis induction, and metastasis inhibition. These biological events may be involved in crosstalk with the epidermal growth factor receptor (EGFR), which belongs to the ErbB family of receptor tyrosine kinases. Several cancer therapeutic approaches employ small molecules capable of inhibiting tyrosine kinases (e.g., gefitinib). According to more recent reports, combining gefitinib with chemotherapeutics, such as cisplatin, seems to be more effective than monotherapy. The present study aimed to assess the molecular mechanism of the potential anti-proliferative activity of individual and combined treatments with DMU-214 and gefitinib in SCC-25 and CAL-27 human tongue cancer cell lines. We showed for the first time the anti-cancer effects of DMU-214, gefitinib, and their combination in tongue cancer cells triggered via cell cycle arrest, apoptosis induction, and inhibition of the EGFR signaling pathway. The anti-proliferative effects of DMU-214 and gefitinib are also suggested to be related to the EGFR and EGFRP (phosphorylated epidermal growth factor receptor) expression status since we found significantly weaker cytotoxic activity of the compounds tested in SCC-25 cells, which overexpressed EGFR and EGFRP proteins.
New Therapies to Correct the Cystic Fibrosis Basic Defect
Authors: Bergeron Christelle , Cantin André M.
Published: June 2021

Rare diseases affect 400 million individuals worldwide and cause significant morbidity and mortality. Finding solutions for rare diseases can be very challenging for physicians and researchers. Cystic fibrosis (CF), a genetic, autosomal recessive, multisystemic, life-limiting disease does not escape this sad reality. Despite phenomenal progress in our understanding of this disease, treatment remains difficult. Until recently, therapies for CF individuals were focused on symptom management. The discovery of the cystic fibrosis transmembrane conductance regulator (CFTR) gene and its product, a protein present at the apical surface of epithelial cells regulating ion transport, allowed the scientific community to learn about the basic defect in CF and to study potential therapies targeting the dysfunctional protein. In the past few years, promising therapies with the goal to restore CFTR function became available and changed the lives of several CF patients. These medications, called CFTR modulators, aim to correct, potentialize, stabilize or amplify CFTR function. Furthermore, research is ongoing to develop other targeted therapies that could be more efficient and benefit a larger proportion of the CF community. The purpose of this review is to summarize our current knowledge of CF genetics and therapies restoring CFTR function, particularly CFTR modulators and gene therapy.
Uremic Toxins and Their Relation with Oxidative Stress Induced in Patients with CKD
Authors: Pieniazek Anna , Bernasinska-Slomczewska Joanna , Gwozdzinski Lukasz
Published: June 2021

The presence of toxins is believed to be a major factor in the development of uremia in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). Uremic toxins have been divided into 3 groups: small substances dissolved in water, medium molecules: peptides and low molecular weight proteins, and protein-bound toxins. One of the earliest known toxins is urea, the concentration of which was considered negligible in CKD patients. However, subsequent studies have shown that it can lead to increased production of reactive oxygen species (ROS), and induce insulin resistance in vitro and in vivo, as well as cause carbamylation of proteins, peptides, and amino acids. Other uremic toxins and their participation in the damage caused by oxidative stress to biological material are also presented. Macromolecules and molecules modified as a result of carbamylation, oxidative stress, and their adducts with uremic toxins, may lead to cardiovascular diseases, and increased risk of mortality in patients with CKD.
Species Identification of Wireworms (Agriotes spp.; Coleoptera: Elateridae) of Agricultural Importance in Europe: A New “Horizontal Identification Table”
Authors: Furlan Lorenzo , Benvegnù Isadora , Bilò María Fabiana , Lehmhus Jörn , Ruzzier Enrico
Published: June 2021

Wireworms are yellowish soil-dwelling larvae that damage a wide range of arable crops. The most common wireworms found in European cultivated fields (except for the Caucasus) belong to the genus Agriotes (Coleoptera: Elateridae). In several European countries, environment-impacting insecticides are applied on a prophylactic basis to control them. However, before any treatment can be applied, European legislation requires that an assessment is done when pest population levels exceed a damage threshold. The threshold substantially depends on wireworm species, thus quick reliable larval identification is needed to implement the appropriate integrated pest management practices. Furthermore, research into non-chemical strategies involves carrying out tests with live and identified wireworms. Thus, thousands of wireworms were observed in a bid to identify live larvae so that larval density could be assessed and compared with species-specific thresholds before sowing, and laboratory experiments were carried out. This work led to a horizontal identification table that allows for quick and accurate identification of live larvae. This key, unlike traditional dichotomous keys, simultaneously considers a set of multiple discriminating morphological characters in order of stability. The key can be reliably used by less experienced users and, once minimum familiarity is acquired, most larvae can be identified rapidly, with high precision.
Ce2O3/BiVO4 Embedded in rGO as Photocatalyst for the Degradation of Methyl Orange under Visible Light Irradiation
Authors: Onwudiwe Damian C. , Phadi Boitumelo M. , Oyewo Opeyemi A.
Published: June 2021

A p–n heterojunction semiconductor structure composed of Ce3O4 and BiVO4 has been synthesized and then incorporated into reduced graphene oxide (rGO) by the hydrothermal method. The ternary composites were characterized by X-ray diffraction, transmission electron microscopy (TEM), scanning electron microscopy (SEM), electron diffraction spectroscopy (EDS), and UV–vis spectroscopy. The efficiency of the composites as photocatalysts was determined by studying the oxidative degradation of methyl orange in aqueous solution under visible light irradiation. The effect of parameters such as pH, catalyst loading, and concentration of the dye solution was examined in order to determine their influence on the photocatalytic activity of the composites. The composite incorporated into reduced graphene oxide presented the highest percentage (above 90%) in 2 h time, attributed to the effect of the increased surface area. The process of the enhanced photocatalytic activity has been discussed based on the energy band positions of the nanoparticles within the composite.
The Influence of State and Trait Anxiety on the Achievement of a Virtual Reality Continuous Performance Test in Children and Adolescents with ADHD Symptoms
Authors: Areces Débora , Rodríguez Celestino , García Trinidad , Cueli Marisol , González-Castro Paloma
Published: June 2021

The three types of presentations of ADHD often co-occur with other disorders, anxiety being one of the most prevalent. For this reason and because there are few studies that have examined the influence of anxiety on attentional activities, this study aims to determine how internalizing difficulties (anxiety levels) can influence performance in a virtual reality continuous performance test. The study used a non-probabilistic clinical sample comprising 68 boys (66%) and 35 girls (34%) aged between 6 and 16 (M = 12.24; SD = 2.45) who had been referred to clinical services for the evaluation of ADHD symptoms. Once informed consent was given, the children were administered the STAI-C scale and a virtual reality continuous performance test by expert researchers. Hierarchical regression models showed that only state anxiety demonstrated significant explanatory power over attentional variables. These findings confirm how important it is for children to feel relaxed when they undergo psychological evaluation tests, as otherwise the individual’s intervention design would be based on biased data. Similarly, the findings also suggested an effect of IQ in the interpretation of continuous performance scores.
Improved Temporal Trends of Vaccination Coverage Rates in Childhood after the Mandatory Vaccination Act, Italy 2014–2019
Authors: Sabbatucci Michela , Odone Anna , Signorelli Carlo , Siddu Andrea , Maraglino Francesco , Rezza Giovanni
Published: June 2021

Maintaining high vaccine coverage (VC) for pediatric vaccinations is crucial to ensure herd immunity, reducing the risk of vaccine-preventable diseases (VPD). The Italian vaccination Law (n. 119/2017) reinforced mandates for polio, diphtheria, tetanus, and hepatitis B, extending the mandate to pertussis, Haemophilus influenzae type b, chickenpox, measles, mumps, and rubella, for children up to 16 years of age. We analyzed the national temporal trends of childhood immunization rates from 2014 to 2019 to evaluate the impact of the mandatory reinforcement law set in 2017 as a sustainable public health strategy in Italy. In a 3-year period, 9 of the 10 compulsory vaccinations reached the threshold of 95% and VC for chicken pox increased up to 90.5%, significantly. During the same period, the recommended vaccinations (against meningococcus B and C, pneumococcus, and rotavirus) also recorded a significant increase in VC trends. In conclusion, although the reinforcement of compulsory vaccination generated a wide public debate that was amplified by traditional and social media, the 3-year evaluation highlights positive results.
Risk Indicators Affecting the Survival of the Mandibular First Molar Adjacent to an Implant at the Mandibular Second Molar Site: A Retrospective Study
Authors: Park Won-Bae , Kwon Koo-Hyun , Hwang Kyung-Gyun , Han Ji-Young
Published: June 2021

This study aimed to compare the survival of mandibular first molars (MnM1s) adjacent to implants placed in mandibular second molar sites (ImM2s) with MnM1s adjacent to mandibular second molars (MnM2s) and to investigate risk indicators affecting the survival of MnM1s adjacent to ImM2s. A total of 144 patients who had MnM1s adjacent to ImM2s and MnM1s adjacent to MnM2s on the contralateral side were included in this study. Clinical variables and radiographic bone levels were evaluated. The survival of MnM1s adjacent to ImM2s or MnM2s was evaluated using a Kaplan–Meier analysis and Cox proportional hazards model. The 5-year cumulative survival rates of MnM1s adjacent to ImM2s and MnM2s were 85% and 95%, respectively. MnM1s adjacent to ImM2s of the internal implant-abutment connection type had higher multivariate hazard ratios (HR) for loss. MnM1s that had antagonists with implant-supported prostheses also had higher HR for loss. The multivariate HR for the loss of MnM1s adjacent to ImM2s with peri-implant mucositis was 3.74 times higher than MnM1s adjacent to healthy ImM2s. This study demonstrated several risk indicators affecting the survival of MnM1s adjacent to ImM2s. It is suggested that supportive periodontal and peri-implant therapy combined with meticulous occlusal adjustment can prolong the survival of MnM1s and ImM2s.
Blinatumomab in Pediatric Acute Lymphoblastic Leukemia—From Salvage to First Line Therapy (A Systematic Review)
Authors: Queudeville Manon , Ebinger Martin
Published: June 2021

Acute lymphoblastic leukemia is by far the most common malignancy in children, and new immunotherapeutic approaches will clearly change the way we treat our patients in future years. Blinatumomab is a bispecific T-cell-engaging antibody indicated for the treatment of relapsed/refractory acute lymphoblastic leukemia (R/R-ALL). The use of blinatumomab in R/R ALL has shown promising effects, especially as a bridging tool to hematopoietic stem cell transplantation. For heavily pretreated patients, the response to one or two cycles of blinatumomab ranges from 34% to 66%. Two randomized controlled trials have very recently demonstrated an improved reduction in minimal residual disease as well as an increased survival for patients treated with blinatumomab compared to standard consolidation treatment in first relapse. Current trials using blinatumomab frontline for high-risk patients or as a consolidation treatment post-transplant will show whether efficacy is even higher in less heavily pretreated patients. Due to the distinct pattern of adverse events compared to high-dose conventional chemotherapy, blinatumomab could play an important role for patients with a risk for severe chemotherapy-associated toxicities. This systematic review discusses all published results for blinatumomab in children as well as all ongoing clinical trials.
Understanding the Coupling Effect between Lignin and Polybutadiene Elastomer
Authors: Hait Sakrit , De Debapriya , Ghosh Prasenjit , Chanda Jagannath , Mukhopadhyay Rabindra , Dasgupta Saikat , Sallat Aladdin , Al Aiti Muhannad , Stöckelhuber Klaus Werner , Wießner Sven , Heinrich Gert , Das Amit
Published: June 2021

From an environmental and economic viewpoint, it is a win–win strategy to use materials obtained from renewable resources for the production of high-performance elastomer composites. Lignin, being a renewable biomass, was employed as a functional filler material to obtain an elastomer composite with a higher degree of mechanical performance. In the presence of a suitable coupling agent, an elevated temperature was preferred for the reactive mixing of lignin with polybutadiene rubber (BR). It is quite fascinating that the mechanical performance of this composite was comparable with carbon black-filled composites. The extraordinary reinforcing behavior of lignin in the BR matrix was understood by an available model of rubber reinforcement. In rubber composite preparation, the interfacial interaction between polybutadiene rubber and lignin in the presence of a coupling agent enabled the efficient dispersion of lignin into the rubber matrix, which is responsible for the excellent mechanical properties of the rubber composites. The rubber composites thus obtained may lead to the development of a sustainable and cost-effective end product with reliable performance. This novel approach could be implemented in other type of elastomeric materials, enabling a genuine pathway toward a sustainable globe.
The Effect of Bottom Profile Dimples on the Femoral Head on Wear in Metal-on-Metal Total Hip Arthroplasty
Authors: Jamari J. , Ammarullah Muhammad Imam , Saad Amir Putra Md , Syahrom Ardiyansyah , Uddin Mohammad , van der Heide Emile , Basri Hasan
Published: June 2021

Wear and wear-induced debris is a significant factor in causing failure in implants. Reducing contact pressure by using a textured surface between the femoral head and acetabular cup is crucial to improving the implant’s life. This study presented the effect of surface texturing as dimples on the wear evolution of total hip arthroplasty. It was implemented by developing finite element analysis from the prediction model without dimples and with bottom profile dimples of flat, drill, and ball types. Simulations were carried out by performing 3D physiological loading of the hip joint under normal walking conditions. A geometry update was initiated based on the patient’s daily routine activities. Our results showed that the addition of dimples reduced contact pressure and wear. The bottom profile dimples of the ball type had the best ability to reduce wear relative to the other types, reducing cumulative linear wear by 24.3% and cumulative volumetric wear by 31% compared to no dimples. The findings demonstrated that surface texturing with appropriate dimple bottom geometry on a bearing surface is able to extend the lifetime of hip implants.
Dual-Axial Tool Servo Diamond Turning of Hierarchical Micro-Nano-Structured Surfaces
Authors: Pu Xiaonan , Zhu Zihui , Chen Li , Huang Peng , Wang Yulin , Zhu Zhiwei
Published: June 2021

This paper reports on a dual-axial tool servo diamond turning method for the one-step fabrication of hierarchical micro-nano-structured surfaces. With respect to the dual-axial servo motion (XZ), the z-axis motion can generate the primary surface with a complex shape, and the x-axis motion is used to synchronously form the secondary structure via controlling the residual tool marks. The toolpath determination algorithm for the developed turning method is described in detail, and the effect of the machining parameters on the basic feature and sizes of the generated secondary structures is investigated through conducting the numerical simulation for both toolpath and surface generation. The simulation result indicates that the additional x-axial motion is effective for the deterministic generation of a variety of secondary structures. Finally, taking advantage of an ultra-precision lathe with a self-developed tri-axial FTS, a hierarchical surface with high accuracy is practically generated.
Generation of Efficient Iso-Planar Printing Path for Multi-Axis FDM Printing
Authors: Bi Danjie , Xie Fubao , Tang Kai
Published: June 2021

The emerging multi-axis fused deposition modeling (FDM) printing process is a powerful technology for fabricating complicated 3D models that otherwise would require extensive support structures or suffer the severe stair-case effect if printed on a conventional three-axis FDM printer. However, because of the addition of two rotary axes which enables the printing nozzle to change its orientation continuously, and the fact that the printing layer is now curved, determining how a nozzle printing path to cover the layer becomes a non-trivial issue, since the rotary axes of the printer in general have a much worse kinematic capacity than the linear axes. In this paper, specifically targeting robotic printing, we first propose an efficiency indicator called the material deposition rate which considers both the local geometry of the layer surface and the kinematic capacities of the printer. By maximizing this indicator globally, a best drive plane direction is found, and then the classic iso-planar method is adopted to generate the printing path for the layer, which not only upholds the specified printing quality but also strives to maximize the kinematic capacities of the printer to minimize the total printing time. Preliminary experiments in both computer simulation and physical printing are carried out and the results give a positive confirmation on the proposed method.
What Is New in Biomarker Testing at Diagnosis of Advanced Non-Squamous Non-Small Cell Lung Carcinoma? Implications for Cytology and Liquid Biopsy
Authors: Hofman Paul
Published: June 2021

The discovery and clinical validation of biomarkers predictive of the response of non-squamous non-small-cell lung carcinomas (NS-NSCLC) to therapeutic strategies continue to provide new data. The evaluation of novel treatments is based on molecular analyses aimed at determining their efficacy. These tests are increasing in number, but the tissue specimens are smaller and smaller and/or can have few tumor cells. Indeed, in addition to tissue samples, complementary cytological and/or blood samples can also give access to these biomarkers. To date, it is recommended and necessary to look for the status of five genomic molecular biomarkers (EGFR, ALK, ROS1, BRAFV600, NTRK) and of a protein biomarker (PD-L1). However, the short- and more or less long-term emergence of new targeted treatments of genomic alterations on RET and MET, but also on others’ genomic alteration, notably on KRAS, HER2, NRG1, SMARCA4, and NUT, have made cellular and blood samples essential for molecular testing. The aim of this review is to present the interest in using cytological and/or liquid biopsies as complementary biological material, or as an alternative to tissue specimens, for detection at diagnosis of new predictive biomarkers of NS-NSCLC.
Funneliformis mosseae Improves Growth and Nutrient Accumulation in Wheat by Facilitating Soil Nutrient Uptake under Elevated CO2 at Daytime, Not Nighttime
Authors: Shi Songmei , Luo Xie , Wen Miao , Dong Xingshui , Sharifi Sharifullah , Xie Deti , He Xinhua
Published: June 2021

The concurrent effect of elevated CO2 (eCO2) concentrations and arbuscular mycorrhizal fungi (AMF) on plant growth, carbon (C), nitrogen (N), phosphorus (P) and potassium (K) accumulations in plant and soil is largely unknown. To understand the mechanisms of eCO2 and mycorrhization on wheat (Triticum aestivum) performance and soil fertility, wheat seedlings were grown under four different CO2 environments for 12 weeks, including (1) ambient CO2 (ACO2, 410/460 ppm, daytime/nighttime), (2) sole daytime eCO2 (DeCO2, 550/460 ppm), (3) sole nighttime eCO2 (NeCO2, 410/610 ppm), and (4) dual or continuous daytime/nighttime eCO2 ((D + N)eCO2, 550/610 ppm), and with or without AMF (Funneliformis mosseae) colonization. DeCO2, NeCO2 and (D + N)eCO2 generally significantly increased shoot and root biomass, plant C, N, P and K accumulation, soil invertase and urease activity, but decreased shoot and root N, P and K concentrations, and soil available N, P and K. Compared with non-AMF, AMF effects on above-mentioned characteristics were significantly positive under ACO2, DeCO2 and (D + N)eCO2, but negative on plant biomass, C, N, P and K accumulation under NeCO2. Overall, AMF colonization alleviated soil nutrient constraints on plant responses to DeCO2, while NeCO2 decreased AMF’s beneficial effects on plants. These results demonstrated that an integration of AMF’s benefits to plants under factual field DeCO2 and/or NeCO2 will be critical for managing the long-term consequence of future CO2 rising on global cropping systems.
Development of a Simple and Robust Whole Blood Assay with Dual Co-Stimulation to Quantify the Release of T-Cellular Signature Cytokines in Response to Aspergillus fumigatus Antigens
Authors: Lauruschkat Chris D. , Page Lukas , White P. Lewis , Etter Sonja , Davies Helen E. , Duckers Jamie , Ebel Frank , Schnack Elisabeth , Backx Matthijs , Dragan Mariola , Schlegel Nicolas , Kniemeyer Olaf , Brakhage Axel A. , Einsele Hermann , Loeffler Juergen , Wurster Sebastian
Published: June 2021

Deeper understanding of mold-induced cytokine signatures could promote advances in the diagnosis and treatment of invasive mycoses and mold-associated hypersensitivity syndromes. Currently, most T-cellular immunoassays in medical mycology require the isolation of mononuclear cells and have limited robustness and practicability, hampering their broader applicability in clinical practice. Therefore, we developed a simple, cost-efficient whole blood (WB) assay with dual α-CD28 and α-CD49d co-stimulation to quantify cytokine secretion in response to Aspergillus fumigatus antigens. Dual co-stimulation strongly enhanced A. fumigatus-induced release of T-cellular signature cytokines detectable by enzyme-linked immunosorbent assay (ELISA) or a multiplex cytokine assay. Furthermore, T-cell-dependent activation and cytokine response of innate immune cells was captured by the assay. The protocol consistently showed little technical variation and high robustness to pre-analytic delays of up to 8 h. Stimulation with an A. fumigatus lysate elicited at least 7-fold greater median concentrations of key T-helper cell signature cytokines, including IL-17 and the type 2 T-helper cell cytokines IL-4 and IL-5 in WB samples from patients with Aspergillus-associated lung pathologies versus patients with non-mold-related lung diseases, suggesting high discriminatory power of the assay. These results position WB-ELISA with dual co-stimulation as a simple, accurate, and robust immunoassay for translational applications, encouraging further evaluation as a platform to monitor host immunity to opportunistic pathogens.
Continuous Subcutaneous Apomorphine Infusion in Parkinson’s Disease: A Single-Center, Long-Term Follow-Up Study of the Causes for Discontinuation
Authors: Henriksen Tove , Staines Harry
Published: June 2021

(1) Background: Subcutaneous apomorphine infusion (SCAI) is one of the three main treatment options for motor fluctuations in advanced Parkinson’s disease (PD). The adherence to SCAI is generally considered to be low due to adverse events and because it is perceived as a treatment option to be used for a limited period only. We evaluated the reasons for discontinuation of SCAI in relation to when patients stopped treatment. (2) Methods: We reviewed the medical records of PD patients treated with SCAI at a single center, capturing patient demographics and the reasons for cessation of SCAI. (3) Results: 101 patients were included in the analysis, with a median time on treatment of 6.34 years. The main reasons for stopping SCAI were adverse events, death, and dissatisfaction with treatment. In the first 6 years of treatment, the predominant side effects leading to discontinuation were somnolence and hallucinations. (4) Conclusions: We suggest that SCAI can be an effective long-term treatment option for advanced PD, but it requires careful patient selection, a high level of communication with the patient and carer, and rigorous monitoring of the effects of treatment and for any adverse events so they can be promptly managed.
The Effect of the Family Type and Home Literacy Environment on the Development of Literacy Skills by Bi-/Multilingual Children in Cyprus
Authors: Karpava Sviatlana
Published: June 2021

Literacy is a broad term that includes reading and writing abilities, as well as cognitive skills that are socially and culturally constructed. Thus, it is essential to take the family context and home literacy environment (HLE) into consideration when discussing literacy. HLE affects reading and writing development via (in)formal literacy experiences focused on the development of oral language and code skills via exposure, child-centered and instructed activities. In this study, we investigated the effect of the family type (intermarriage/exogamous and co-ethnic/endogamous) and HLE on the development of literacy in bi-/multilingual children in Cyprus. The results of the study, which was based on qualitative methodology (questionnaires, interviews and observations), showed that there was a close relationship between the family type, family language policy (FLP), the HLE and the development of children’s language and literacy skills which, in addition, depended on their socioeconomic status (SES), the level of the parents’ education, life trajectories and experience, linguistic and cultural identities, status in the society, future plans for residency, and the education and careers of their children. Overall, Russian-speaking parents in immigrant contexts realized the importance of (early) child literacy experiences at home, as well as of multiliteracy and multimodality, and attempted to enhance these experiences both in Russian and in the majority language(s), mainly via formal, didactic activities focused on code skills.