bluuebunny/crossref_metadata_2025 huggingface dataset:
Initiator-Directed Transcription: Fission Yeast Nmtl Initiator Directs Preinitiation Complex Formation and Transcriptional Initiation
Authors: Rojas Diego A. , Urbina Fabiola , Valenzuela-Pérez Lucía , Leiva Lorenzo , Miralles Vicente J. , Maldonado Edio
Published: January 2022

The initiator element is a core promoter element encompassing the transcription start site, which is found in yeast, Drosophila, and human promoters. This element is observed in TATA-less promoters. Several studies have defined transcription factor requirements and additional cofactors that are needed for transcription initiation of initiator-containing promoters. However, those studies have been performed with additional core promoters in addition to the initiator. In this work, we have defined the pathway of preinitiation complex formation on the fission yeast nmt1 gene promoter, which contains a functional initiator with striking similarity to the initiator of the human dihydrofolate reductase (hDHFR) gene and to the factor requirement for transcription initiation of the nmt1 gene promoter. The results show that the nmt1 gene promoter possesses an initiator encompassing the transcription start site, and several conserved base positions are required for initiator function. A preinitiation complex formation on the nmt1 initiator can be started by TBP/TFIIA or TBP/TFIIB, but not TBP alone, and afterwards follows the same pathway as preinitiation complex formation on TATA-containing promoters. Transcription initiation is dependent on the general transcription factors TBP, TFIIB, TFIIE, TFIIF, TFIIH, RNA polymerase II, Mediator, and a cofactor identified as transcription cofactor for initiator function (TCIF), which is a high-molecular-weight protein complex of around 500 kDa. However, the TAF subunits of TFIID were not required for the nmt1 initiator transcription, as far as we tested. We also demonstrate that other initiators of the nmt1/hDHFR family can be transcribed in fission yeast whole-cell extracts.
Antisense and Gene Therapy Options for Duchenne Muscular Dystrophy Arising from Mutations in the N-Terminal Hotspot
Authors: Wilton-Clark Harry , Yokota Toshifumi
Published: January 2022

Duchenne muscular dystrophy (DMD) is a fatal genetic disease affecting children that is caused by a mutation in the gene encoding for dystrophin. In the absence of functional dystrophin, patients experience progressive muscle deterioration, leaving them wheelchair-bound by age 12 and with few patients surviving beyond their third decade of life as the disease advances and causes cardiac and respiratory difficulties. In recent years, an increasing number of antisense and gene therapies have been studied for the treatment of muscular dystrophy; however, few of these therapies focus on treating mutations arising in the N-terminal encoding region of the dystrophin gene. This review summarizes the current state of development of N-terminal antisense and gene therapies for DMD, mainly focusing on exon-skipping therapy for duplications and deletions, as well as microdystrophin therapy.
Mild Hypophagia and Associated Changes in Feeding-Related Gene Expression and c-Fos Immunoreactivity in Adult Male Rats with Sodium Valproate-Induced Autism
Authors: Pal Tapasya , Laloli Kathryn , Moscrip Cushla , Olszewski Pawel , Klockars Anica
Published: January 2022

A core yet understudied symptom of autism is aberrant eating behaviour, including extremely narrow food preferences. Autistic individuals often refuse to eat despite hunger unless preferred food is given. We hypothesised that, apart from aberrant preference, underfeeding stems from abnormal hunger processing. Utilising an adult male VPA rat, a model of autism, we examined intake of ‘bland’ chow in animals maintained on this diet continuously, eating this food after fasting and after both food and water deprivation. We assessed body weight in adulthood to determine whether lower feeding led to slower growth. Since food intake is highly regulated by brain processes, we looked into the activation (c-Fos immunoreactivity) of central sites controlling appetite in animals subjected to food deprivation vs. fed ad libitum. Expression of genes involved in food intake in the hypothalamus and brain stem, regions responsible for energy balance, was measured in deprived vs. sated animals. We performed our analyses on VPAs and age-matched healthy controls. We found that VPAs ate less of the ‘bland’ chow when fed ad libitum and after deprivation than controls did. Their body weight increased more slowly than that of controls when maintained on the ‘bland’ food. While hungry controls had lower c-Fos IR in key feeding-related areas than their ad libitum-fed counterparts, in hungry VPAs c-Fos was unchanged or elevated compared to the fed ones. The lack of changes in expression of feeding-related genes upon deprivation in VPAs was in contrast to several transcripts affected by fasting in healthy controls. We conclude that hunger processing is dysregulated in the VPA rat.
Genome-Wide Identification of CCD Gene Family in Six Cucurbitaceae Species and Its Expression Profiles in Melon
Authors: Cheng Denghu , Wang Zhongyuan , Li Shiyu , Zhao Juan , Wei Chunhua , Zhang Yong
Published: January 2022

The carotenoid cleavage dioxygenase (CCD) gene family in plants comprises two subfamilies: CCD and 9-cis-epoxycarotenoid dioxygenase (NCED). Genes in the NCED subfamily are mainly involved in plant responses to abiotic stresses such as salt, low temperature, and drought. Members of the NCED subfamily are the most important rate-limiting enzymes in the biosynthesis of abscisic acid (ABA). In the present study, genome-wide analysis was performed to identify CCD gene members in six Cucurbitaceae species, including watermelon (Citrullus lanatus), melon (Cucumis melo), cucumber (C.sativus), pumpkin (Cucurbita moschata), bottle gourd (Lagenaria siceraria), and wax gourd (Benincasa hispida). A total of 10, 9, 9, 13, 8, 8 CCD genes were identified in the six species, respectively, and these genes were unevenly distributed in different chromosomes. Phylogenetic analysis showed that CCD genes of the six species clustered into two subfamilies: CCD and NCED, with five and three independent clades, respectively. The number of exons ranged from 1 to 15, and the number of motifs were set to 15 at most. The cis-acting elements analysis showed that a lot of the cis-acting elements were implicated in stress and hormone response. Melon seedlings were treated with salt, low temperature, drought, and ABA, and then tissue-specific analysis of CCDs expression were performed on the root, stem, upper leaf, middle leaf, female flower, male flower, and tendril of melon. The results showed that genes in CCD family exhibited various expression patterns. Different CCD genes of melon showed different degrees of response to abiotic stress. This study presents a comprehensive analysis of CCD gene family in six species of Cucurbitaceae, providing a strong foundation for future studies on specific genes in this family.
Acknowledgment to Reviewers of Geomatics in 2021
Authors:   Geomatics Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
The Association of Health-Related Factors with Leisure-Time Physical Activity among Adults with COPD: A Cross-Sectional Analysis
Authors: Chen Mei-Lan , Chen Li-Sheng , Chen Yen Tzu , Gardenhire Douglas S.
Published: January 2022

This study aimed to examine the association of health attitudes, health appraisals and affective experience to leisure-time physical activity in adults with chronic obstructive pulmonary disease (COPD). Cross-sectional analyses were conducted with a sample of 274 adults with COPD drawn from the second wave of the Midlife in the United States (MIDUS 2) Study. Chi-square analyses and independent t-tests were used to test the differences between physically active and inactive COPD patients (active group versus inactive group) for all study variables. Multiple logistic regression was used to examine the association of each study variable with leisure-time physical activity. The results showed that there were significant differences between the active and inactive groups in terms of age, education, functional limitations, health attitudes, health appraisals and affective experience. After controlling for socio-demographic variables and functional limitations, beliefs about the importance of physical fitness and strength for a good life and comparative health appraisals were significantly related to physical activity. However, neither negative nor positive affect was associated with physical activity status. Modifiable factors, such as health attitudes toward physical fitness and strength, as well as health appraisals, should be considered for developing effective physical activity promotion interventions among COPD patients.
Immediate Effects of Single-Session High-Velocity Training for Lateral Trunk Movement on Gait Function in Early Postoperative Patients after Total Hip Arthroplasty: A Nonrandomized Controlled Trial
Authors: Honma Keisuke , Yano Yuki , Yamamoto Saki , Ohmine Toshimitsu , Wanaka Hideyuki , Senzaki Kazuma , Kanayama Atsuki , Oonishi Hiroyuki , Iwata Akira
Published: January 2022

Background: Total Hip Arthroplasty (THA) is an effective method for relieving pain and improving gait function. However, THA patients demonstrate slow gait speed at discharge. Rehabilitation programs after THA require the immediate improvement of gait speed early in the postoperative period. To examine the immediate effects of seated side tapping training (SSTT), which focuses on lateral trunk movement and movement velocity, on gait function in early postoperative THA patients, the methods were as follows: The SSTT group performed five repetitions of a task in which they moved their trunks laterally to alternately touch markers to their left and right side as quickly as possible 10 times in a seated position. One set of SSTT lasted approximately 3 min. The control group rested in a seated position for 10 min. Results: Significant interactions were observed for gait speed, stride time, and stride time coefficient of variability. The SSTT group demonstrated significant pre-post-intervention improvement in gait speed, stride time, and coefficient of variability. Conclusions: SSTT improved both gait speed and gait stability and can be performed easily and safely. Therefore, single-session high-velocity trunk training may be an effective method to improve gait function immediately in early postoperative THA patients.
Acknowledgment to Reviewers of Hearts in 2021
Authors:   Hearts Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Hydrogen-Rich Water Prevents Dehydration-Induced Cellular Oxidative Stress and Cell Death in Human Skin Keratinocytes
Authors: Xiao Li , Miwa Nobuhiko
Published: January 2022

Hypohydration is linked to increased risk of a variety of diseases and can be life-threatening, especially in elderly populations. Dehydration induces cellular damage partially through the production of reactive oxygen species (ROS) in cells, tissues and organs. Hydrogen molecules are known to convert ROS to harmless water. Therefore, theoretically hydrogen-rich water (HW) might eliminate dehydration-induced ROS and reverse its harmful effects in cells. In this in vitro study, we demonstrated that air-drying for 5 min could induce ROS generation in both nucleus and cytoplasm of human keratinocytes HaCaT as quantified by CellROX® Green/Orange reagents (Thermo Fisher Scientific, Waltham, Massachusetts, U.S.), respectively. Conversely, when the air-drying time was increased to 10 and 20 min, HaCaT cells lost the ability to produce ROS. Scanning electron microscopic (SEM) images showed that 10 min air-drying could induce severe membrane damage in HaCaT cells. PrestoBlue assay showed that, when HaCaT cells were air-dried for 20 min, cell viability was decreased to 27.6% of the control cells 48 h later. However, once HaCaT cells were pretreated with HW-prepared media, dehydration-induced intracellular ROS, cell membrane damage and cell death were significantly reduced as compared with double distilled water (DDW) under the same conditions. In conclusion, our data suggested that HW can decrease dehydration-induced harmful effects in human cells partially through its antioxidant capacity.
Understanding the Value of Tourism to Seniors’ Health and Positive Aging
Authors: Qiao Guanghui , Ding Liu , Xiang Keheng , Prideaux Bruce , Xu Jinyi
Published: January 2022

The value of tourism has been included in studies of active aging, and the existing public health implications of the physical and mental health effects of tourism among seniors are recognized as important issues. This study uses a mixed methods research approach to explore the effects of tourism value on the health and mortality risk of older adults, referred to in this paper as seniors. Survival analysis and cohort analysis are combined with the narrative analysis of in-depth interviews with eight convenience-sampled seniors to construct a narrative framework of seniors’ active aging tourism and its intrinsic drivers. The study found that the intrinsic framework of active aging tourism values for seniors has a three-stage continuum, an interaction, and orientation characteristics. There are six intrinsic key value drivers of tourism value and public health for seniors. In addition, this study identifies the personal characteristics and strengths of seniors as important influences on tourism value practices for active aging and public health. This study provides a positive psychological and behavioral research direction for existing research on the value of tourism in active aging. It provides an empirical basis for exploring the intrinsic mechanisms of tourism and public health.
Youth Athletes’ Perception of Existence and Prevalence of Aggression and Interpersonal Violence and Their Forms in Serbia
Authors: Matić Radenko M. , Milovanović Ivana M. , Banjac Brigita , Šošo Biljana Milošević , Vuković Jovan , Gentile Ambra , Drid Patrik
Published: January 2022

Aggression and interpersonal violence (IV) against children and youth are issues in contemporary society. The current study reports on the youth athletes’ perception of the aggression and IV prevalence and its manifestation forms in a Serbian context. The sample included athletes (N = 2091) between the age of 11 and 18 years. Data were collected through an ad-hoc questionnaire created by the authors. Respondents’ answers to introductory questions about the frequency of IV indicated its absence (78.1%). However, the answers to the questions about specific forms of violent peer behavior indicated forms as well as the time and place where IV most often occurs. They underlined that IV takes place mainly after training or competition and during sports camps; and that the dressing room is the most favorable place for these behaviors. They also confirmed that the most prevalent subtypes of IV are psychological (roughly 40%) and physical (approximately 30%). The respondents’ opinions and experiences about IV (psychological, physical, sexual) revealed that factors such as gender, location, and parents’ education level may influence the IV manifestation. Highlighting the prevalence and the most frequently manifesting types of aggression and IV could help in awareness-raising of these social issues.
Effects of a 12-Week Diet versus Diet plus Aerobic and Resistance Exercise Program on Acylated and Desacylated Ghrelin, and Ghrelin O-Acyltransferase in Adolescent Girls with Obesity
Authors: Kim Hyun Jun , Tak Young Jin , Lee Sang Yeoup , Seo Jeong Pyo
Published: January 2022

This study investigated the effects of a 12-week diet versus diet plus aerobic and resistance exercise programme on acylated ghrelin (AG), desacylated ghrelin (DAG), and ghrelin O-acyltransferase (GOAT) concentrations in girls with obesity. We randomised 30 adolescents with obesity to a 12-week aerobic and resistance exercise group (EG) or a control group (CG). At baseline and at 4, 8, and 12 weeks, we measured their body composition, lipid profile, glucose, AG, DAG, and GOAT concentrations. In the EG, the body fat percentage decreased by 2.37% and was significantly lower than that in the CG. The DAG concentrations significantly increased by 48.3% and 27.4% in the EG and CG, respectively. At 4, 8, and 12 weeks, DAG concentrations were significantly higher in the EG than in the CG. AG concentrations were higher at week 12 than at baseline in both groups. In both groups, the GOAT concentrations increased at weeks 8 and 12; however, no between-group differences were observed in the changes in GOAT concentrations. This study showed increased DAG concentrations and non-significant changes in AG and GOAT concentrations after a 12-week aerobic and resistance exercise programme in girls with obesity. These findings suggest that an aerobic and resistance exercise programme influences appetite-regulating hormones, mainly through changes in DAG concentrations.
Adolescents’ Perceptions of Harmfulness of Tobacco and Tobacco-like Products in Finland
Authors: El-Amin Salma , Kinnunen Jaana M. , Rimpelä Arja
Published: January 2022

During the recent years, new tobacco and tobacco-like products, e.g., e-cigarettes, have emerged on the market. Adolescents often underestimate health risks in general, including those concerning tobacco. Little is known of adolescents’ perceptions of health risks of the newer products. Our paper compares adolescents’ perceptions of harmfulness of cigarettes, e-cigarettes, snus, water pipes, and nicotine in Finland, a country with a long history of strict tobacco control policy. Online surveys to nationally representative samples of 12–18-year-olds were conducted in 2017 and 2019, with 7578 answering the surveys. Only 3% of boys and 2% of girls did not agree that cigarettes are harmful to health. The percentages were slightly higher for snus (6% and 3%, respectively) and nicotine (12%, 8%) but much higher for e-cigarettes (30%, 22%) and water pipes (36%, 38%). Those who used the product, whose parents were smokers or had lower education, and whose school performance was lower, less often agreed with the harmful health effects of the products. Our results showed that adolescents understood the harmfulness of older tobacco products better than the harmfulness of the newer ones. Our results also showed the need to strengthen health education and fix adolescents’ misperceptions of the health effects of the newer products.
Digital Implant Planning in Patients with Ectodermal Dysplasia: Clinical Report
Authors: Bohner Lauren , Vinayahalingam Shankeeth , Kleinheinz Johannes , Hanisch Marcel
Published: January 2022

Ectodermal dysplasia may severely affect the development of jaw growth and facial appearance. This case report describes the treatment of two patients suffering from ectodermal dysplasia, both treated with dental implant-fixed restorations by means of computer-guided surgery. Two patients presented to our clinic with congenital malformation of the jaw as a manifestation of ectodermal dysplasia, showing oligodontia and alveolar ridge deficit. Clinical examination revealed multiple unattached teeth and a need for prosthetic therapy. For both cases, dental implants were placed based on a computer-guided planning. A surgical guide was used to determine the positioning of the dental implants according to the prosthetic planning, which allowed for a satisfactory aesthetic and functional outcome. Computer-guided implant placement allowed predictable treatment of complex cases with satisfactory aesthetic and functional results. Adequate surgical and prosthetic planning is considered critical for treatment success.
Online Information of COVID-19: Visibility and Characterization of Highest Positioned Websites by Google between March and April 2020—A Cross-Country Analysis
Authors: Kłak Anna , Grygielska Jolanta , Mańczak Małgorzata , Ejchman-Pac Ewelina , Owoc Jakub , Religioni Urszula , Olszewski Robert
Published: January 2022

Background: The WHO has used the term “infodemic” to describe the vast amount of false and true information that was making it difficult for people to find reliable information when they needed it. The infodemic spreads faster than COVID-19 itself. The main objective of the study was to characterize and analyze content about COVID-19 returned by Google during the pandemic and compare it between countries. Methods: The study was conducted between 30 March and 27 April 2020. The information was searched through local Google websites using the “COVID-19”, “Coronavirus”, “SARS-CoV-2” and “fake news” keywords. The search was conducted in Australia, France, Germany, Italy, Poland, Singapore, Spain, UK and the USA. The total number of the analyzed webpages was 685. Results: The most frequent types were News websites 47% (324/685) and Governmental 19% (131/685) while the least were Health portals 2% (17/685) and Scientific journals 5% (35/635), p < 0.001. United States and Australia had the highest share of Governmental websites. There was a positive correlation between the amount of preventive information and a number of SARS-CoV-2 infections in countries. The higher the number of tests performed, the higher was the amount of information about prevention available online. Conclusions: Online information is usually available on news and government websites and refers to prevention. There were differences between countries in types of information available online. The highest positioned (the first 20) websites for COVID-19, Coronavirus and SARS-CoV-2 keywords returned by Google include true information.
Music Therapy Supports Children with Neurological Diseases during Physical Therapy Interventions
Authors: Kobus Susann , Bologna Franziska , Maucher Ines , Gruenen Daniel , Brandt Ramona , Dercks Martin , Debus Otfried , Jouini Eva
Published: January 2022

Recent research found evidence supporting music therapy for children with neurological diseases during their hospitalized neurological early rehabilitation to promote their development during physical therapy. We hypothesized that live music therapy might improve vital signs during a physical therapy session. Seventeen children received live music therapy during the physical therapy session twice a week. Two more physical therapy sessions per week were held without music therapy. Heart rate, respiratory rate and oxygen saturation were recorded from 15 min before to 15 min after the therapy sessions. Physical therapy interventions showed changes in heart rate, respiratory rate and oxygen saturation between, before and after the sessions with or without music therapy. Live music therapy was effective for the vital signs during the intervention. We observed significantly lower heart and respiratory rates and higher oxygen saturation during physical therapy intervention with live music therapy in general (mean differences −8.0 beats per min; −0.8 breaths per min and +0.6%). When physical therapy was applied without music therapy children’s heart rates increased by 8.5 beats per min and respiratory rates increased by 1.0 breaths per min. Live music therapy leads to a decrease in heart and respiratory rates and an increase in oxygen saturation in children with neurological diseases during physical therapy with live music therapy. Music therapy supports the children in physical therapy interventions during their hospitalization.
“She Has the Japanese Style”: Parenting by Japanese Immigrant Women in Korea from the Perspective of Their Children
Authors: Heo Nayoung
Published: January 2022

This study looks into the parenting beliefs and behavior of female Japanese immigrants, who have stayed in Korea for more than 20 years, and attempts to explore factors for their distinct parenting style compared to their Korean counterparts. In Korea and Japan, a mother’s parenting is seen as crucial, while paternal responsibility is considered minimal. There are few studies that look into parenting patterns of Japanese immigrant women in Korea, especially from the perspective of their children. The study is based on in-depth interviews and participant observation in 2014 with 21 young Japanese-Korean adults recruited through snowball sampling. In raising children, the priorities of mothers were not academic but instead focused on children’s autonomy, wholesome personality, religiosity, various life experiences, maintaining health, and non-intervention. The mothers’ own cultural background and strong Japanese identity, limited interaction with Korean mothers or exclusive interaction with Japanese mothers, the role of the father figure as only a breadwinner, and mothers’ strong devotion to church activities tend to influence their parenting. An exploration of the mechanisms behind the differences in their styles of parenting is important before judging their parenting to be improper or maladjusted. Understanding parenting styles of families from diverse backgrounds helps to understand what society will look like in the near future.
Students Sense of Belonging and Academic Performance via Online PBL: A Case Study of a University in Hong Kong during Quarantine
Authors: Liu Xunqian , Yang Yi , Ho Jessica Ws
Published: January 2022

Innovative educational adaptations have been essential during the COVID-19 pandemic. Against the backdrop of school closures in Hong Kong resulting from unrest and COVID-19, this study proposed using problem-based learning (PBL) in online courses and empirically examined the influence of the PBL learning methodology on online learners’ sense of classroom belonging and academic performance. A total of 44 sophomores pursuing electrical and electronic engineering majors and taking “Practical Chinese for engineering students” as a compulsory course at the University of Hong Kong participated in the study. They were divided evenly between the experimental group C1 and the control group C2 (22 in each). We implemented online PBL learning for the experimental group, C1, and traditional online learning for the control group, C2. Quantitative data were collected via an achievement test and a scale on sense of classroom community. Qualitative data were obtained through a semi-structured focus group discussion. The quantitative results showed that the students who received the PBL learning method scored higher on tests, experienced a stronger sense of classroom belonging, and had closer connections with each other. A content analysis of student interviews revealed that implementation of the PBL learning method in online teaching could strengthen students’ classroom interactions and that the enhancement of their mutual connections could stimulate learning and improve learning efficiency. Overall, this study finds that the PBL learning method is effective in promoting students’ deep active learning and sense of community in the online environment.
Public Awareness of Bowel Cancer Risk Factors, Symptoms and Screening in Tasmania, Australia: A Cross-Sectional Study
Authors: Lee Simone M. , Versace Vincent L. , Obamiro Kehinde
Published: January 2022

Tasmania has one of the highest bowel cancer incidence and death rates in the world. Public awareness of risk factors, symptoms, and early detection of bowel cancer is important for minimising the burden of disease. This study measured awareness levels of bowel cancer risk factors, symptoms and screening in Tasmania. An online survey of 3703 participants aged 18 years and older found that alcohol consumption, low physical activity levels, and having diabetes were the least known risk factors for bowel cancer. Over half of all participants were unaware the risk of bowel cancer increased with age, and 53 percent were not confident they would notice a bowel cancer symptom. Over a third of survey respondents did not know that screening commenced at the age of 50. The results indicate that a targeted campaign to increase bowel cancer awareness in Tasmania may help reduce the high rates of morbidity and mortality from the disease.
Social Determinants of Health and Depression among African American Adults: A Scoping Review of Current Research
Authors: Yelton Brooks , Friedman Daniela B. , Noblet Samuel , Lohman Matthew C. , Arent Michelle A. , Macauda Mark M. , Sakhuja Mayank , Leith Katherine H.
Published: January 2022

Depression in the United States (US) is increasing across all races and ethnicities and is attributed to multiple social determinants of health (SDOH). For members of historically marginalized races and ethnicities, depression is often underreported and undertreated, and can present as more severe. Limited research explores multiple SDOH and depression among African American adults in the US. Guided by Healthy People (HP) 2030, and using cross-disciplinary mental health terminology, we conducted a comprehensive search to capture studies specific to African American adults in the US published after 2016. We applied known scoping review methodology and followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. From 12,315 initial results, 60 studies were included in our final sample. Most studies explored the HP 2030 Social and Community Context domain, with a heavy focus on discrimination and social support; no studies examined Health Care Access and Quality. Researchers typically utilized cross-sectional, secondary datasets; no qualitative studies were included. We recommend research that comprehensively examines mental health risk and protective factors over the life course within, not just between, populations to inform tailored health promotion and public policy interventions for improving SDOH and reducing racial and ethnic health disparities.
Psychometric Properties of the Hebrew Modified Dental Anxiety Scale in Adult Israeli Population
Authors: Shacham Maayan , Greenblatt-Kimron Lee , Humphris Gerry , Ben-Ezra Menachem , Mijiritsky Eitan
Published: January 2022

Dental anxiety results in the neglect of oral hygiene and poor oral health, requiring an accurate screening tool for dental practitioners to evaluate dental anxiety. The Modified Dental Anxiety Scale (MDAS) is frequently used cross-culturally. The present study aimed to assess the reliability and validity of the Hebrew version of the MDSA. A total of 553 (mean age 35.87 years, SD = 13.14) Israeli participants were recruited through means of social media, mailing lists, and forums. The sample was randomly divided into two population sets. Dental anxiety was evaluated using the Hebrew version of the MDAS. The psychometric evaluation consisted of exploratory factor analysis (study 1, n = 274) and confirmatory factor analysis (study 2, n = 279). Cronbach’s alpha coefficient was used to assess internal consistency. Results showed high internal consistency (0.93) for the Hebrew version of the MDAS. Confirmatory factor analysis showed a single factor solution. Findings demonstrated 13.4% of Israeli participants with dental anxiety. Younger participants, females, participants with lower education, lower income, and more religious participants reported higher dental anxiety. In conclusion, the Hebrew version of the MDAS demonstrated high reliability and validity. It is recommended to use the Hebrew version of the MDAS to evaluate dental anxiety in Israeli dental settings.
Policies and Toxicological Screenings for No Drug Addiction: An Example from the Civil Aviation Workforce
Authors: Treglia Michele , Pallocci Margherita , Ricciardi-Tenore Giorgio , Baretti Flavio , Bianco Giovanna , Castellani Paola , Pizzuti Fabrizio , Ottaviano Valeria , Passalacqua Pierluigi , Leonardi Claudio , Coppeta Luca , Messineo Agostino , Tittarelli Roberta
Published: January 2022

Introduction: Since 2008, Italian legislators, with the aim of ensuring public safety, have made it mandatory for an occupational doctor (OD) to assess specific categories of workers to exclude those who may have consumed drugs of abuse. Due to the relevance of work activities relating to the civil aviation and airport sector, a policy based on the use of training and information tools, as well as a health surveillance protocol, has been undertaken since 2009. Materials and methods: A total of 61,008 workers at a commercial airline underwent health surveillance between 2009 and 2019. Following ≤24 h notification, their urine was screened for opiates, cocaine, cannabinoids, amphetamines, methamphetamines, and methylenedioxymethamphetamine (MDMA) using an immunochemical test. Positive results were confirmed using Gas Chromatography-Mass Spectrometry (GC/MS) or Liquid Chromatography -Mass Spectrometry (LC/MS). In confirmed cases, the workers were declared unfit and sent to a specialized laboratory for a second level analysis. Results: Positive results, initially >1%, have halved in the last four years (<0.5%). The percentage of positive results was consistently very low among pilots and, moreover, the rare positive cases detected were due to a cross-reactivity phenomena. The highest and most discontinuous percentages seen occurred in the population undergoing a pre-employment examination. Regarding the types of substance used, a prevalence of cannabis (58.52%) and cocaine (35.2%) use was observed. Conclusions: The data presented indicate that the air transport sector, in all its components (ground workers and air crews), has a very limited number of substance abusers, and this number tends to decrease over time and with work seniority. Another aspect of particular interest, and which is more specific to toxicology, concerns the detection of cross-reactivity in urinary immunochemical screening between the antibodies to drugs of abuse and certain other drugs, such as anti-inflammatories or antibiotics; as well as foods, and other commonly used substances.
Prevalence and Sociodemographic Factors Associated with Overweight and Obesity among Adults in Poland: A 2019/2020 Nationwide Cross-Sectional Survey
Authors: Stoś Katarzyna , Rychlik Ewa , Woźniak Agnieszka , Ołtarzewski Maciej , Jankowski Mateusz , Gujski Mariusz , Juszczyk Grzegorz
Published: January 2022

Detailed characteristics of the weight status of the population is necessary for the effective prevention of health disorders, e.g., through personalized nutrition education. This study aimed to characterize weight status and identify sociodemographic factors associated with overweight/obesity in a representative sample of adult inhabitants of Poland. This cross-sectional study was carried out from July 2019 to February 2020 on a representative nationwide sample of individuals aged 18+ in Poland. The study consisted of two parts: questionnaire survey and anthropometric measurements. The body mass index was calculated. Data on 1831 adults (50.3% females; mean age 51.7 ± 19.9 years) were included in this analysis. The prevalence of overweight was 42.2% (52.4% among males and 32.0% among females). Of the 1831 participants, 16.4% were obese (16.5% of males and 16.2% of females). Out of 11 factors analyzed in this study, only 5 were significantly associated with overweight/obesity. Males, older participants, occupationally active individuals, those living in rural areas and individuals with at least one chronic disease had greater odds of overweight/obesity. This study demonstrated a high prevalence of overweight and obesity among adults in Poland. This is the most up-to-date representative study on nutritional status carried out before the COVID-19 pandemic.
Methodological Considerations for Movement Education Interventions in Natural Environments for Primary School Children: A Scoping Review
Authors: Petrigna Luca , Thomas Ewan , Scardina Antonino , Rizzo Federica , Brusa Jessica , Camarazza Giovanni , Galassi Claudia , Palma Antonio , Bellafiore Marianna
Published: January 2022

Background: Education is the ideal setting for carrying out projects to improve primary students’ capacities. In recent years, interventions in natural environments have been more frequently proposed, but there is still a lack of standardization, making deeper study of the topic necessary. This review aims to report on what previous scientific research has been carried out, and eventually, to propose standard operating procedures for future interventions. Methods: This is a scoping review that adopted the PRISMA guidelines. Primary school children have been included, and the interventions had to be proposed adopting nature as the primary element of the learning process. Results: A total of 19 studies have been included, and a wide range of methodological differences has been detected regarding the included intervention protocols. Conclusions: Learning in nature is a feasible intervention that, despite the high heterogeneity of interventions, demonstrates positive outcomes in the learning sphere of children.
Behavioral Medicine Physiotherapy in the Context of Return to Work for Chronic Pain: A Single-Case Experimental Design Study
Authors: Zetterberg Hedvig , Flink Ida , Spörndly-Nees Sören , Wagner Sofia , Karlsten Rolf , Åsenlöf Pernilla
Published: January 2022

Effective interventions are needed for return-to-work (RTW) for individuals with chronic pain on long-term sick leave. In this study, a behavioral medicine physiotherapy protocol was systematically replicated and added to workplace components. The intervention was evaluated for fidelity and effects on target activities and work ability. A single-case experimental design was used with five participants. Daily and weekly ratings of personalized target activities at work as well as work ability were carried out throughout the study period of 26–28 weeks. Effects of the behavioral medicine physiotherapy intervention were evaluated for each individual using visual analysis of displayed graphs and quantitative non-overlap methods. Goal achievement for target activities was reviewed. Three participants completed the intervention. The results indicated an effect from the behavioral medicine physiotherapy intervention on task-specific self-efficacy for target activities, but no consistent effect on experience of target activities or work ability. All three participants had increased function in target activities in line with pre-defined goals. Fidelity to the intervention manual was good. Behavioral medicine physiotherapy can be successfully adapted to work disability and was here replicated in an RTW context for individuals with chronic pain. The intervention protocol should be further evaluated in large-scale studies.
On the Supervision of a Saturated SIR Epidemic Model with Four Joint Control Actions for a Drastic Reduction in the Infection and the Susceptibility through Time
Authors: De la Sen Manuel , Ibeas Asier , Alonso-Quesada Santiago
Published: January 2022

This paper presents and studies a new epidemic SIR (Susceptible–Infectious–Recovered) model with susceptible recruitment and eventual joint vaccination efforts for both newborn and susceptible individuals. Furthermore, saturation effects in the infection incidence terms are eventually assumed for both the infectious and the susceptible subpopulations. The vaccination action on newborn individuals is assumed to be applied to a fraction of them while that on the susceptible general population is of linear feedback type reinforced with impulsive vaccination actions (in practice, very strong and massive vaccination controls) at certain time points, based on information on the current levels of the susceptible subpopulation. Apart from the above vaccination controls, it is also assumed that the average of contagion contacts can be controlled via intervention measures, such as confinements or isolation measures, social distance rules, use of masks, mobility constraints, etc. The main objectives of the paper are the achievement of a strictly decreasing infection for all time periods and that of the susceptible individuals over the initial period if they exceed the disease-free equilibrium value. The monitoring mechanism is the combined activation of intervention measures to reduce the contagion contacts together with the impulsive vaccination to reduce susceptibility. The susceptibility and recovery levels of the disease-free equilibrium point are suitably prefixed by the design of the regular feedback vaccination on the susceptible subpopulation.
Characteristics of Clinics without National Health Insurance Contracts: A Nationwide Survey in Taiwan
Authors: Lu Pei-Jyun , Liu Jui-Yao , Ma Hsin , Chen Tzeng-Ji , Chou Li-Fang , Hwang Shinn-Jang
Published: January 2022

Based on the 1978 Alma-Ata Declaration, the key to achieving health for all is primary health care, and many countries have established various comprehensive health care systems. Because of the financial toll of a public health care system, government-sponsored public health insurance is not universally accepted. This study used Taiwan as the backdrop to understand why many health clinics have chosen not to accept the National Health Insurance (NHI), despite it covering 99.93% of the country’s population. The clinics’ operational details were garnered from the datasets of Taiwan’s open government data platforms and checked against the list of contracting clinics within the NHI. Of 10,907 Western medicine primary care clinics in 2016, as many as 9846 (90.3%) clinics had signed contracts with the NHI. The remaining 1061 noncontracting clinics were distributed in urban (94.5%, n = 1003), suburban (4.9%, n = 52), and rural/remote areas (0.6%, n = 6). The NHI did not have contracts with 183 plastic surgery, 88 internal medicine, and 85 surgery clinics. In conclusion, nearly one-tenth of clinics practiced independently of the NHI in Taiwan. Their reasons for declining the contract and practices for delivering their services deserve further studies.
The Prevalence of Antimicrobial Resistant Neisseria gonorrhoeae in Papua New Guinea: A Systematic Review and Meta-Analysis
Authors: Willie Barne , Sweeney Emma L. , Badman Steven G. , Chatfield Mark , Vallely Andrew J. , Kelly-Hanku Angela , Whiley David M.
Published: January 2022

Neisseria gonorrhoeae antimicrobial resistance (NG AMR) has become an urgent concern globally. The World Health Organization, the United States of America Centers for Disease Control, and other regulators have called to improve resistance-testing methods to enhance NG AMR surveillance. NG AMR surveillance remains critical in informing treatment; unfortunately, this is often lacking in settings with limited resources, such as Papua New Guinea (PNG). We conducted a systematic review and a prevalence meta-analysis, and provided an overview of NG AMR in PNG. We showed the lack of NG AMR data in the last decade, and emphasized the need for NG AMR surveillance in PNG. Since NG AMR testing by the NG culture method is unreliable in PNG, we suggested using molecular tests to complement and enhance NG AMR surveillance.
Knowledge of COVID-19 and Its Relationship with Preventive Behaviors and Vaccination among Adults in Northern Thailand’s Community
Authors: Pothisa Tharadon , Ong-Artborirak Parichat , Seangpraw Katekaew , Tonchoy Prakasit , Kantow Supakan , Auttama Nisarat , Boonyathee Sorawit , Choowanthanapakorn Monchanok , Bootsikeaw Sasivimol , Panta Pitakpong , Dokpuang Dech
Published: January 2022

(1) Background: the 2019 coronavirus disease outbreak (COVID-19) has posed a major threat to public health and had a significant impact on all areas of people’s lives. Vaccines against COVID-19 have been developed to control the disease, and an array of personal hygiene measures has been introduced. As a result, information that will support and promote vaccination among populations as well as other health measures against COVID-19 are urgently needed. The goal of this research was to look into the knowledge about COVID-19 and how it relates to preventive behaviors and vaccination among people living in rural areas of northern Thailand. (2) Methods: a cross-sectional study was performed in four upper northern provinces of Thailand. A total of 1524 participants were recruited using the probability sampling technique. Questionnaires were distributed to collect data on general health information, as well as knowledge and preventive behaviors regarding COVID-19. (3) Results: more than half (55.9%) of the participants were female and had not received the COVID-19 vaccine (67.2%). Their mean age was 44.13 years. The majority had moderate COVID-19 knowledge and engaged in preventive behaviors (65.5% and 42.0%, respectively). A linear regression model showed that the COVID-19 knowledge score was related to the COVID-19 preventive behaviors score, with a standardized coefficient of 0.510, after adjusting for age, underlying disease, and body mass index (B = 2.64; 95%CI = 2.42, 2.87). Binary logistic regression revealed that after controlling for age, education, occupation, financial status, and current disease (AOR = 1.87; 95%CI = 1.64–2.13), the score of COVID-19 knowledge was significantly associated with having the COVID-19 vaccine. (4) Discussion: knowledge of COVID-19 is very important for people in rural regions to engage in COVID-19 prevention behaviors and vaccination. Relevant government agencies and health network partners should support proactive education campaigns emphasizing the risk of contracting the disease and its severity in order to promote vaccination against COVID-19 among unvaccinated groups. These campaigns can highlight COVID-19’s positive benefit-risk balance in the short and long term and ensure public safety measures against COVID-19.
Promoting and Risk Factors of Nurses’ Hardiness Levels during the COVID-19 Pandemic: Results from an Italian Cohort
Authors: Baldassini Rodriguez Samuele , Bardacci Yari , El Aoufy Khadija , Bazzini Marco , Caruso Christian , Giusti Gian Domenico , Mezzetti Andrea , Bambi Stefano , Guazzini Andrea , Rasero Laura
Published: January 2022

Aim: Few studies in the literature specifically address the hardiness of nurses during the COVID-19 pandemic. Thus, the primary aim of this study was to assess the impact of COVID-19 on the hardiness levels in an Italian cohort of nurses. The secondary aims were to assess the level of hardiness in nurses directly caring for patients with COVID-19 and to verify the presence of related risk and promoting factors. Methods: A descriptive and explorative study was performed through an online survey from March to July 2020. The survey was composed of a multiple answer questionnaire with open, closed, and semi-closed-ended questions. Hardiness and anxiety were assessed using two psychometric instruments: the Dispositional Resilience Scale (DRS-15) and State-Trait Anxiety Inventory (STAI-Y). Results: A total of 1250 nurses completed the questionnaire entirely (92.3% of respondents). The average length of service was 17.8 ± 11.5 years. A decrease in the hardiness was recorded after the first wave of COVID-19 if compared to the baseline (mean Δ DRS-15 total = 1.3 ± 5.0), whereas in the subsample of nurses caring for COVID-19 patients, the total hardiness level decreased more consistently (mean Δ DRS Total = 1.9 + 5.3). Multivariate analysis showed that high levels of anxiety were risk factors for reducing hardiness. In contrast, anxiety, when associated with a greater length of service, was a promoting factor for the increase in hardiness. Conclusions: The correlation between anxiety and years of length of service appears to be pivotal. Future research should focus on the role of anxiety to establish its actual role as a predictor of hardiness.