bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrating reflection into a mobile-assisted reading program for learning English as a second language in China
Authors: Wang Fei , Yuizono Takaya , Wang Tzu-Yang , Kim Eunyong , Lu Yaqin
Published: January 2023

The application of mobile technology in language education is gaining increasing momentum for its potential benefits, and scholars cast attention to issues such as learner motivation, learning effects and learner behaviors in the mobile learning process. Reflection is an essential part in learning as it can record learner behaviors, cultivate self-awareness of knowledge construction, facilitate cognitive growth, and promote academic achievement. Despite of the wide approval of reflection, not much study has been done concerning the application of reflection in mobile language learning process. Therefore, this study aims to investigate students’ perception of a mobile-assisted reading program facilitated with reflective activities as well as their preferences for reflection modes adopted. The participants were 60 students from two classes in a Chinese college. Students read passages on mobile applications and completed a reflection in one mode every two weeks. Four modes (paper journal reflection, e-journal reflection, audio reflection, and collaborative reflection) were adopted in the study. The study lasted approximately nine weeks. At the end of the program, all students were required to complete an anonymous questionnaire concerning their learning perception. In addition, ten students were selected randomly to attend a semi-structured interview. A pretest and a posttest were conducted to observe students’ language gains. A combination of quantitative and qualitative analysis was conducted with the data obtained. Results showed students generally approved of the effect of this mobile-assisted reading and their reading proficiency improved significantly after the program. In addition, most students favored reflective practices as a good way to stimulate interest, deepen understanding and promote reflective and summarizing abilities, but they didn’t consider it a good method to monitor the learning process in the mobile-assisted reading program. As for the preference for reflection mode, most students favored traditional paper reflection and audio reflection, while collaborative reflection and e-journal reflection received the least support. The findings provided implications for educators and app designers. For educators, based on the understanding of students’ age, learning experience, and possible preferences, they may create a good reflective environment with technical and instructional support, and then provide two or three popular modes for students to reflect on whatever they read. For app developers, some preferable reflection modes facilitated with stimulative measures may be offered to cater to more learners to conduct reflective activities.
Investigating the effects of gender and scaffolding in developing preschool children’s computational thinking during problem-solving with Bee-Bots
Authors: Angeli Charoula , Georgiou Kyriakoula
Published: January 2023

The research community has embraced computational thinking as an essential skill to develop in school and academic settings. Many researchers argue that computational thinking should be developed in the context of programming and robotic activities in all educational levels of education, starting from early childhood education. However, the factors related to developing computational thinking in preschool education are still under study. Furthermore, not too many empirical investigations provide evidence about the development of computational thinking in young children. The present study examined the effects of scaffolding and gender in developing young children’s sequencing and decomposition skills - two of the five skills that constitute computational thinking. The results indicated statistically significant effects about the type of scaffolding on children’s computational thinking in favor of the children assigned to the experimental groups. Lastly, boys outperformed girls on all occasions, indicating that gender effects exist. The authors conclude that researchers need to design teaching interventions in such a way so they have mathemagenic outcomes for all learners irrespective of gender. Finally, the authors conclude with implications and future research directions.
Androgen deprivation therapy and radiotherapy in intermediate-risk prostate cancer: A systematic review and meta-analysis
Authors: Chen Jiuzhou , Yuan Yan , Fang Miao , Zhu Youqi , Sun Xueqing , Lou Yufei , Xin Yong , Zhou Fengjuan
Published: January 2023

Objectives

Androgen deprivation therapy combined with radiotherapy for intermediate-risk prostate cancer is still a matter of debate. We conducted a meta-analysis to evaluate the necessity of androgen deprivation therapy combined with radiotherapy for intermediate-risk prostate cancer patients.

Methods

A comprehensive literature search of articles was performed in PubMed, Embase, Cochrane library, Web of Science, Chinese National Knowledge Infrastructure, Chinese Biological Medicine, Wanfang, and VIP Databases published between February 1988 and April 2022. Studies comparing the survival of patients diagnosed with intermediate-risk prostate cancer who were treated with androgen deprivation therapy combined with radiotherapy or radiotherapy alone were included. Data were extracted and analyzed with the RevMan software (version 5.3) and the Stata software (version 17).

Results

Six randomized controlled trials and nine retrospective studies, including 6853 patients (2948 in androgen deprivation therapy combined with radiotherapy group and 3905 in radiotherapy alone group) were enrolled. Androgen deprivation therapy combined with radiotherapy did not provide an overall survival (HR 1.12, 95% CI 1.01-1.12, p=0.04) or biochemical recurrence-free survival (HR 1.23, 95% CI 1.09-1.39, P=0.001) advantage to intermediate-risk prostate cancer patients.

Conclusion

Androgen deprivation therapy combined with radiotherapy did not show some advantages in terms of overall survival and biochemical recurrence-free survival and radiotherapy alone may be the effective therapy for intermediate-risk prostate cancer patients.

Systematic review registration

https://inplasy.com/inplasy-2022-8-0095/, identifier 202280095.
Cueing roles of new energy vehicle manufacturers’ technical capability and reputation in influencing purchase intention in China
Authors: Lu Zhigang , Cai Zhilin
Published: January 2023

Promoting new energy vehicle (NEV) is one of the main ways to save energy and reduce transport emissions, China has provided substantial subsidies for this since 2009. With the impending end of the subsidy policy ending in 2022, NEV manufacturers need to strengthen their competitiveness to continuously attract customers. Under the framework of cue utilization theory, this study takes NEV manufacturers’ technical capability as an intrinsic cue and reputation as an extrinsic cue to explore the mechanism in which two cues stimulate customers’ perceptions of travel quality and brand value, and subsequently motivate purchase intention. Based on a sample of 207 respondents from China, proposed hypotheses have been tested using Likert scale questionnaires through SPSS and AMOS. Structural equation modeling techniques were used to analyze the measurement scales and variable relationships. The results show that manufacturers’ reputation is more influential on both perceived travel quality and perceived brand value than technical capability; Technological turbulence plays a moderating role in the influence between perceived brand value and purchase intention. This article provides references for deepening related theories, and pragmatic insights for manufacturer strategic response and government policy making.
Model predictive power control of a heat pipe cooled reactor
Authors: Huang Jiajun , Sun Peiwei , Pu Songmao
Published: January 2023

Heat pipe cooled reactor (HPR) has broad application prospects in deep space exploration, deep-sea submarine exploration, and other scenarios due to the small size, high inherent safety, and easy modularization and expansion. However, the HPR conducts thermal energy through evaporation and condensation of the working fluid inside the heat pipe. This feature makes the HPR a large time-delay system. If the power control system adopts the conventional PID algorithm, there will be a long settling time. Therefore, the model predictive control algorithm is proposed for the power control system to improve the control performance. The HPR linear model, which is developed by linearization of its nonlinear model, is chosen as the predictive model. The optimal control value is obtained by solving the optimization problem based on the predictive model and the electric power feedback value. The discrepancy between the predive model and the actual system response results in the presence of steady-state error. To solve this problem, an integral controller is added to eliminate the error. The appropriate control system parameters are tuned by the trial and error method. The proposed control system has satisfactory control performance, which can significantly shorten the settling time. The model predictive control can effectively overcome the influence of the large time-delay characteristic.
Cooling performance in a minichannel heat sink with different triangular pin-fins configurations
Authors: Zhao Kai , Sun Xiaoxia , Xia Yuanqing , Li Qiangqiang , Shen Lili , Lin Min
Published: January 2023

With the continuous progress of automotive new energy technology, the motor has become an important part of the power system, and the heat dissipation of insulated-gate bipolar transistors (IGBT) determines the reliability of the power system. Minichannel structure can be added to the thermal management system of new energy vehicles to improve the heat transfer capacity. Due to the growth of the boundary layer in the smooth minichannel flow channel, the cooling performance improvement was limited. Pin-fins and rib structures were used to break the boundary layer and increased the heat transfer area to enhance the heat transfer capacity. In this study, a numerical simulation model of minichannel with triangular pin-fins with different rotation angles was established and calculated using the SST k-omega method. The temperature field, velocity field, pressure, and vortex distribution under different configurations were discussed in detail. The jet area formed by the prism wall and the side wall of the minichannel would impact the wall and reduce the growth of the boundary layer. However, the stagnation area generated in the center and corner will reduce the improvement of heat transfer capacity. The thermo-hydraulic characteristics of different configurations at different Reynolds numbers (Re), such as Nusselt number (Nu), Darcy friction resistance coefficient (f), and performance evaluation criterion (PEC), were analyzed. As Re increased, the best and worst configurations changed, the best configuration changed from the 90°–120° structure to the 120°–120° structure, and the worst configuration changed from the 75°–60° to the 60°–60° structure. When the Re = 663, the influence of the front and rear rotation angle on the cooling performance was explored. When the rotation angle was closer to 60°, the cooling performance of the minichannel was better. And the closer the rotation angle was to 120°, the cooling performance was better. This has a reference effect on the design of minichannel heat sinks.
Optical and molecular diversity of dissolved organic matter in sediments of the Daning and Shennong tributaries of the Three Gorges Reservoir
Authors: Wang Kai , Fang Hongwei , He Guojian , Huang Lei , Cui Zhenghui , Gao Qifeng , Xu Song , Wang Dianchang , Wu Xinghua , He Ding
Published: January 2023

Introduction: Damming significantly modifies the function of natural river networks and influences sediment dynamics with a reservoir’s operation. The dissolved organic matter (DOM) in reservoir sediments severely affects carbon flow from land to sea. However, the properties of DOM (e.g., quantity and quality) in reservoir sediments and their relationship with carbon cycling remain unclear as complex reservoir construction interrupts the environmental processes.

Methods: This study characterizes the optical and molecular properties of sediment water-extractable organic matter (WEOM) in the Daning and Shennong tributaries of the world’s largest reservoir—the Three Gorges Reservoir (TGR)—by applying optical techniques and ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).

Results and Discussion: We first assessed the link between light-absorbing components and the individual molecules in WEOM, which were significantly different than DOM in water and indicated that there might be an intrinsic variation between DOM in sediment and in water. Then, with the unique optical–molecular property linkage assessed, multiple sources (autochthonous and terrestrial) were identified, and a declining trend of terrestrial and recalcitrant WEOM was revealed from the tributaries upstream to downstream. Finally, through covariance analysis of the properties between WEOM and sediment particles, we demonstrated that the WEOM dynamic was most likely regulated by hydrologic sorting-induced particle size and mineral composition variations of sediment. Moreover, assessment between lability and WEOM molecular properties suggested that the WEOM dynamic likely contributes to carbon burial in the reservoir. This study emphasizes the influence of dam construction on organic matter accumulation and riverine carbon cycling.
Setting the Forestry Carbon Sink Insurance compensation standard for reducing losses from forest fires: An empirical study estimating CO2 emissions from forest fires in a sample of 15 provinces
Authors: Lv Huina , Shi Anqichen
Published: January 2023

Introduction:Forest fires have contributed to increasingly serious global warming by great amount of CO2emissions and are seen as a loss of carbon sink value, which could be reduced by compensating economically via the Forestry Carbon Sink Insurance. However, estimating loss of carbon stock by estimating carbon emissions of forest fire losses is a crucial step of calculating the loss of carbon sink value.

Methods:In this research, method proposed by Seiler and Crutzen (1980) was introduced to estimate the carbon emissions as CO2by in fifteen sample provinces in China by using official data in 2020, which would provide a scientific expectation in future.

Results:Results show the range of carbon released in the sample provinces and we have estimated for the whole country during 2020——the overall amount of carbon released as CO2affected by forest fires reached 35017.42–98486.5t, which can be regarded as a loss of 35017.42–98486.5t of forest carbon sequestration capacity.

Discussion:This study supplies one way of estimating loss of carbon sink value and provides evidence from China that the range of carbon stock loss because of forest fires. In practice, this study supports the forestry authorities to participate in the Forestry Carbon Sink Insurance and provides empirical data to establish compensation standards for insurance companies.
Neighbourhood characteristics, lifestyle factors, and child development: Secondary analysis of the All our families cohort study
Authors: MacKinnon A. L. , Sell H. , Silang K. , Xie E. B. , Jung J. W. , Tough S. , Tomfohr-Madsen L.
Published: January 2023

Background

Neighbourhood characteristics have been found to influence child development, but little is known about lifestyle factors that may moderate this relationship, which can provide modifiable targets for policies and programing. This study investigated the association between neighbourhood characteristics (e.g., deprivation, disorder) during pregnancy and child development at age 5 in relation to various lifestyle factors (e.g., physical activity, parent-child reading, community resource use) during early childhood.

Methods

A secondary analysis was conducted using multilevel modeling of data from the All Our Families cohort, recruited in Canada from 2008 to 2010. Participants self-reported on demographics during pregnancy, lifestyle factors at 3 years, and child development at 5 years using the Ages and Stages Questionnaire (ASQ-3). Neighbourhood deprivation was evaluated using the Vancouver Area Deprivation Index (VANDIX), while disorder was measured using police services' community crime reports.

Results

Geocoded information was available for 2,444 participants. After adjusting for covariates, multilevel modeling indicated a significant negative association between neighbourhood deprivation and overall child development (b = −.726, 95% CI: −1.344, −.120). Parent-child reading was found to be a significant moderator of the effect of neighbourhood disorder (b = .005, 95% CI: .001, .009). There were no statistically significant moderation effects for physical activity or community resource use.

Conclusion

Neighbourhood deprivation during pregnancy is associated with early child development. Parent-child reading may function as a protective factor in the presence of higher neighbourhood disorder. Overall, neighbourhood-level effects should be considered in policies and community programs that promote family and child well-being.
Genotyping by sequencing and a newly developed mRNA-GBS approach to link population genetic and transcriptome analyses reveal pattern differences between sites and treatments in red clover (Trifolium pratense L.)
Authors: Gemeinholzer Birgit , Rupp Oliver , Becker Annette , Strickert Marc , Müller Christina M.
Published: January 2023

The important worldwide forage crop red clover (Trifolium pratense L.) is widely cultivated as cattle feed and for soil improvement. Wild populations and landraces have great natural diversity that could be used to improve cultivated red clover. However, to date, there is still insufficient knowledge about the natural genetic and phenotypic diversity of the species. Here, we developed a low-cost complexity reduced mRNA analysis (mRNA-GBS) and compared the results with population genetic (GBS) and previously published mRNA-Seq data, to assess whether analysis of intraspecific variation within and between populations and transcriptome responses is possible simultaneously. The mRNA-GBS approach was successful. SNP analyses from the mRNA-GBS approach revealed comparable patterns to the GBS results, but due to site-specific multifactorial influences of environmental responses as well as conceptual and methodological limitations of mRNA-GBS, it was not possible to link transcriptome analyses with reduced complexity and sequencing depth to previously published greenhouse and field expression studies. Nevertheless, the use of short sequences upstream of the poly(A) tail of mRNA to reduce complexity are promising approaches that combine population genetics and expression profiling to analyze many individuals with trait differences simultaneously and cost-effectively, even in non-model species. Nevertheless, our study design across different regions in Germany was also challenging. The use of reduced complexity differential expression analyses most likely overlays site-specific patterns due to highly complex plant responses under natural conditions.
The geography of genetic data: Current status and future perspectives
Authors: Peng Xin , Li Qiang , Cheng Zhentao , Huang Xiaolei
Published: January 2023

The biogeography field benefits more and more from the growth and application of genetic data such as nucleotide sequences and whole genomes. It has been perceived by scientists that genetic data may be imbalanced among different geographical regions and taxonomic groups. However, the lack of empirical evidence prevents the understanding of current data volume and distribution of genetic data. Based on the construction of a dataset including records for 365 millions of nucleotide sequences of Animalia, Plantae, and Fungi kingdoms, 6 millions of COI sequences of insects, 77 thousands of COI sequences of mammals, 220 thousands of rbcl sequences of Magnoliopsida, and 44 thousands of ITS sequences of Dothideomycetes, here we present evidence on geographical and taxonomical imbalance of the genetic data, identify major gaps and inappropriate practices in the production, application and sharing of genetic data. We then discuss our perspectives on how to fill up gaps and improve the quantity and quality of genetic data.
Identification of a dysfunctional exon-skipping splice variant in GLUT9/SLC2A9 causal for renal hypouricemia type 2
Authors: Toyoda Yu , Cho Sung Kweon , Tasic Velibor , Pavelcová Kateřina , Bohatá Jana , Suzuki Hiroshi , David Victor A. , Yoon Jaeho , Pallaiova Anna , Šaligová Jana , Nousome Darryl , Cachau Raul , Winkler Cheryl A. , Takada Tappei , Stibůrková Blanka
Published: January 2023

Renal hypouricemia (RHUC) is a pathological condition characterized by extremely low serum urate and overexcretion of urate in the kidney; this inheritable disorder is classified into type 1 and type 2 based on causative genes encoding physiologically-important urate transporters, URAT1 and GLUT9, respectively; however, research on RHUC type 2 is still behind type 1. We herein describe a typical familial case of RHUC type 2 found in a Slovak family with severe hypouricemia and hyperuricosuria. Via clinico-genetic analyses including whole exome sequencing and in vitro functional assays, we identified an intronic GLUT9 variant, c.1419+1G>A, as the causal mutation that could lead the expression of p.Gly431GlufsTer28, a functionally-null variant resulting from exon 11 skipping. The causal relationship was also confirmed in another unrelated Macedonian family with mild hypouricemia. Accordingly, non-coding regions should be also kept in mind during genetic diagnosis for hypouricemia. Our findings provide a better pathogenic understanding of RHUC and pathophysiological importance of GLUT9.
Holistic community-based group parenting programs for mothers with maternal mental health issues help address a growing public health need for a diversity of vulnerable mothers, children and families: Findings from an action research study
Authors: Aylward Paul , Sved Williams Anne
Published: January 2023

Background

Maternal mental illness is a major growing global concern which can affect parenting with serious negative implications for offspring. Group-based parenting programs for mothers which both enhance the parent-child relationship and address mental health symptoms in a supportive social setting may optimise better outcomes for mothers and children. The Acorn program in South Australia draws on attachment theory to integrate dance play, reflective diary keeping and therapeutic letters in a holistic program for a diversity of vulnerable mothers and children aged 1–36 months. The program seeks to nurture and enhance parental wellbeing and the quality of the parent-child relationship for mothers experiencing identified mental health illnesses that impinge upon their parenting. This study presents the evaluation of the program and its effectiveness.

Methods

Action research approach for continuous monitoring and program improvement engaging Acorn program staff in evaluation data collection and interpretation of pre and post self-completion measures and standardized observations. Additional data was collected through a telephone interview of attending mothers 6–8 months after program completion to address sustainable impacts on parenting and wellbeing.

Results

The program engaged 353 diverse vulnerable mothers with their children. Many had profound overlapping mental health issues including borderline personality disorder (BPD) and depression. The quality of the parent-child interaction, parental confidence, competence and enjoyment were enhanced; mothers' wellbeing, ability to cope and lasting social supports were augmented. This occurred for a number of “most vulnerable” subgroups including single mothers, mothers with BPD, mothers from non-English speaking households and those with lower levels of education or household income. Mothers reported sustained improvements in their wellbeing, parenting, social and family lives, and feeling closer to their child as a result of participating in the program.

Conclusions

Given the high prevalence of maternal mental health issues and substantial potential negative consequences for mothers and offspring, the Acorn parenting program offers an effective means of addressing this pressing public health issue potentially helping large numbers of vulnerable mothers and their children. This has additional gravitas in the shadow of COVID-19 due to expanded numbers of those experiencing greater parental stress, isolation and mental illness.
Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
Authors: Li Yanli , Díaz Ivan , Martín-Valls Gerard , Beyersdorf Niklas , Mateu Enric
Published: January 2023

Background

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be infected and cause transplacental infection. The immune effector mechanisms involved are largely unknown.

Methods

The present study investigated the role of cytotoxic lymphocytes, including cytotoxic T cells (CTL), NKT, and NK cells, from blood in preventing PRRSV-1 transplacental infection in vaccinated primiparous sows (two doses vaccinated). Sows from a PRRSV-1 unstable farm were bled just before the last month of gestation (critical period for transplacental infection), then followed to determine whether sows delivered PRRSV-1-infected (n=8) or healthy (n=10) piglets. After that, functions of CTL, NKT, and NK cells in the two groups of sows were compared.

Results

No difference was found through cell surface staining. But upon in vitro re-stimulation with the circulating field virus, sows that delivered healthy piglets displayed a higher frequency of virus-specific CD107a+ IFN-γ-producing T cells, which accumulated in the CD4+ compartment including CD4 single-positive (CD4 SP) and CD4/CD8α double-positive (CD4/CD8α DP) subsets. The same group of sows also harbored a higher proportion of CD107a+ TNF-α-producing T cells that predominantly accumulated in CD4/CD8α double-negative (CD4/CD8α DN) subset. Consistently, CD4 SP and CD4/CD8α DN T cells from sows delivering healthy piglets had a higher virus-specific proliferative response. Additionally, in sows that delivered PRRSV-1-infected piglets, a positive correlation of virus-specific IFN-γ response with average Ct values of umbilical cords of newborn piglets per litter was observed.

Conclusion

Our data strongly suggest that CTL responses correlate with protection against PRRSV-1 transplacental infection, being executed by CD4 T cells (IFN-γ related) and/or CD4/CD8α DN T cells (TNF-α related).
The lung, the niche, and the microbe: Exploring the lung microbiome in cancer and immunity
Authors: Huynh Mai , Crane Meredith J. , Jamieson Amanda M.
Published: January 2023

The lung is a complex and unique organ system whose biology is strongly influenced by environmental exposure, oxygen abundance, connection to extrapulmonary systems via a dense capillary network, and an array of immune cells that reside in the tissue at steady state. The lung also harbors a low biomass community of commensal microorganisms that are dynamic during both health and disease with the capacity to modulate regulatory immune responses during diseases such as cancer. Lung cancer is the third most common cancer worldwide with the highest mortality rate amongst cancers due to the difficulty of an early diagnosis. This review discusses the current body of work addressing the interactions between the lung microbiota and the immune system, and how these two components of the pulmonary system are linked to lung cancer development and outcomes. Bringing in lessons from broader studies examining the effects of the gut microbiota on cancer outcomes, we highlight many challenges and gaps in this nascent field.
Development of a prognostic model to identify the metastatic nasopharyngeal carcinoma patients who may benefit from chemotherapy combination PD-1 inhibitor
Authors: Liu Guo-Ying , Lu Nian , Bei Wei-Xin , Li Wang-Zhong , Liang Hu , Xia Wei-Xiong , Xiang Yan-Qun , Yao He-Rui
Published: January 2023

Background

We aimed to establish a prognostic model to identify suitable candidates for chemotherapy combination PD-1 inhibitor in metastatic nasopharyngeal carcinoma (NPC) patients.

Patients and methods

In this retrospective study, we included 524 patients (192 patients treated with chemotherapy combination PD-1 inhibitor and 332 received chemotherapy alone as first-line regimen) with metastatic NPC between January 2015 and March 2021. We developed a prognostic model to predict progression-free survival (PFS). A model-based trees approach was applied to estimate stratified treatment effects using prognostic scores and two well-matched risk groups (low-risk and high-risk) were created using propensity score matching.

Results

A prognostic nomogram was established with good accuracy for predicting PFS (c-index values of 0.71; 95% confidence interval, 0.66-0.73). The survival curves were significantly different between low-risk and high-risk groups (median PFS: 9.8 vs. 22.8 months, P < 0.001, respectively). After propensity matching analysis, chemotherapy combination PD-1 inhibitor was significantly associated with superior PFS as compared with chemotherapy alone (median PFS, 10.6 versus 9.3 months, P = 0.016) in the high-risk group. However, no significant difference between chemotherapy combination PD-1 inhibitor and chemotherapy was observed (P = 0.840) in the low-risk groups.

Conclusions

Our novel prognostic model was able to stratify patients with metastatic NPC into low-risk or high-risk groups and identify candidates for PD-1 inhibitor therapy. These results are expected to be confirmed by a prospective clinical trial.
Duck cGAS inhibits DNA and RNA virus replication by activating IFNs and antiviral ISGs
Authors: Lin Chang , Zheng Min , Xiao Shifeng , Wang Shao , Zhu Xiaoli , Chen Xiuqin , Jiang Dandan , Zeng Xiancheng , Chen Shaoying , Chen Shilong
Published: January 2023

Cyclic GMP-AMP Synthase (cGAS) is a pivotal adaptor of the signaling pathways involving the pattern recognition receptors and plays an important role in apoptosis and immune regulation. The cGAS function in mammals has been investigated extensively; however, the function of duck cGAS (du-cGAS) in response to viral infections is still unclear. This study aimed to clone the mallard (Anas platyrhynchos) cGAS homolog to investigate the function of duck cGAS (du-cGAS) in host antiviral innate immunity. The results showed that the open reading frame (ORF) region of the du-cGAS gene was 1296 bp, encoding 432 amino acids (aa) and exhibiting similar functional domains with its chicken counterpart. Knockdown of the endogenous du-cGAS by specific sgRNA strongly increased the replication of DNA viruses, including duck adenovirus B2 (DAdV B2) and duck short beak and dwarfism syndrome virus (SBDSV). However, the knockout did not impair the replication of novel duck reovirus (NDRV), an RNA virus. Furthermore, the mRNA expressions of type I interferon (IFNs) and vital interferon-stimulated genes (ISGs) were remarkably reduced in the du-cGAS knockout DEF cell line. Inversely, du-cGAS overexpression greatly activated the transcription of IFN-α, IFN-β, and vital ISGs, and impaired the replication of DAdV B2, SBDSV, and NDRV in the DEF cell line. Importantly, we found that a deletion of 68 aa in the N terminus didn’t impair the antiviral function of du-cGAS. Overexpressing NTase Core, C-Domain (Mab21), or Zinc-Ribbon domain independently had no antiviral effects. Generally, these results reveal that du-cGAS is a vital component of the innate immune system of ducks, with a universal antiviral activity, and provides a useful strategy for the control of waterfowl viral diseases.
Association of low ficolin-2 concentration in cord serum with respiratory distress syndrome in preterm newborns
Authors: Gajek Gabriela , Świerzko Anna S. , Jarych Dariusz , Mikulski Damian , Kobiela Paulina , Chojnacka Karolina , Kufelnicka-Babout Maja , Szala-Poździej Agnieszka , Chrzanowski Jędrzej , Sobczuk Katarzyna , Fendler Wojciech , Matsushita Misao , Domżalska-Popadiuk Iwona , Mazela Jan , Kalinka Jarosław , Sekine Hideharu , Cedzyński Maciej
Published: January 2023

Introduction

Ficolin-2 is a serum pattern recognition molecule, involved in complement activation via the lectin pathway. This study aimed to investigate the association of ficolin-2 concentration in cord blood serum with complications related to premature birth.

Methods

546 premature neonates were included. The concentration of ficolin-2 in cord blood serum was determined by a sandwich TRIFMA method. FCN2 genetic variants were analysed with RFLP-PCR, allele-specific PCR, Sanger sequencing or allelic discrimination using TaqMan probes method.

Findings

Cord blood serum ficolin-2 concentration correlated positively with Apgar score and inversely with the length of hospitalisation and stay at Neonatal Intensive Care Unit (NICU). Multivariate logistic regression analysis indicated that low ficolin-2 increased the possibility of respiratory distress syndrome (RDS) diagnosis [OR=2.05, 95% CI (1.24-3.37), p=0.005]. Median ficolin-2 concentration was significantly lower in neonates with RDS than in premature babies without this complication, irrespective of FCN2 gene polymorphisms localised to promoter and 3’untranslated regions: for patients born <33 GA: 1471 ng/ml vs. 2115 ng/ml (p=0.0003), and for patients born ≥33 GA 1610 ng/ml vs. 2081 ng/ml (p=0.012). Ficolin-2 level was also significantly lower in neonates requiring intubation in the delivery room (1461 ng/ml vs. 1938 ng/ml, p=0.023) and inversely correlated weakly with the duration of respiratory support (R=-0.154, p<0.001). Interestingly, in the neonates born at GA <33, ficolin-2 concentration permitted differentiation of those with/without RDS [AUC=0.712, 95% CI (0.612-0.817), p<0.001] and effective separation of babies with mild RDS from those with moderate/severe form of the disease [AUC=0.807, 95% CI (0.644-0.97), p=0.0002].

Conclusion

Low cord serum ficolin-2 concentration (especially in neonates born at GA <33 weeks) is associated with a higher risk of developing moderate/severe RDS, requiring respiratory support and intensive care.
Morphometric and genetic characterization as tools for selection of Apis mellifera (Hymenoptera: Apidae) stocks in an area of natural hybridization in Argentina
Authors: Litvinoff Leonardo , Menescardi Francisco , Porrini Leonardo , Russo Romina , Liendo María Clara , Nucci Alejandro , Lusarreta Esteban , Ventura Rocio , Espasadin Luna , Monmany-Garzia A. Carolina , Scannapieco Alejandra C. , Galindo-Cardona Alberto
Published: January 2023

Beekeepers around the world select bees’ characteristics that facilitate and favor production. In regions where hybridization among lineages is taking place, this selection is a challenge, given that these regions are “natural laboratories”, where the action of evolutionary processes of a population or species occurs in real time. A natural honeybee (Apis mellifera) hybrid zone exists in Argentina between 28° and 35° South, where Africanized (AHB) and European (EHB) populations converge. In this zone, beekeepers use selected genetic resources of European origin mostly, since the local Africanized bees show a higher defensive behavior, which is not desirable for management. Although EHB colonies have many advantages for honey production, they are not fully adapted to the subtropical climate and are susceptible to certain parasitosis such as varroosis. In addition, both AHB and EHB mate in drone congregation areas (DCAs), where males and virgin queens fly to meet, resulting in variability in the desired characteristics. In this study, we explored the degree of hybridization within a DCA and its reference apiary, located in the province of Entre Ríos, by applying two complementary techniques. First, morphotypes with different degrees of hybridization between European and African subspecies were observed in the reference apiary, indicating a high sensitivity of this morphometric approach to detect hybridization in these populations. Second, a genetic analysis revealed haplotypes of both origins for drones in DCAs, with a higher prevalence of European haplotypes, while all the colonies from the reference apiary exhibited European haplotypes. Overall, our results are in line with the strong impact that commercial beekeeping has on the genetics of DCAs. We show how wing morphometry may be used to monitor hybridization between European and African subspecies, a tool that may be evaluated in other regions of the world where hybridization occurs.
Oceanic response to typhoons in the Northwest Pacific using Aquarius and SMAP data (2011–2020)
Authors: Park Kyung-Ae , Park Jae-Jin , Tang Wenqing
Published: January 2023

Typhoons, such as tropical cyclones, can produce a variety of ocean responses through drastic changes in atmospheric and oceanic environments. However, the uncertainty in satellite salinity data increases during the passage of a typhoon and may limit its potential application. To investigate whether the satellite salinity data can explain oceanic responses to typhoons in the Northwest Pacific, we validated the satellite salinity using Argo float data for the past decade (2011–2020). The Soil Moisture Active Passive (SMAP) and Aquarius salinity were relatively accurate in subtropical regions at low latitudes under high sea surface temperature conditions in summer. This demonstrates the validity of the satellite salinity data in typhoon studies. We analyzed the oceanic responses to 20 representative typhoons over the past decade. Both the Aquarius and SMAP satellites observed a decrease in the SSS on the left side of the typhoon in contrast to the high salinity on the right side of the typhoon. The locations of SSS freshening coincided with those of higher precipitation to the left of the typhoon centers. We also observed that the higher the precipitation rate, the lower the satellite salinity. The ratio of the salinity freshening to the precipitation rate was significant at approximately –0.0401 psu mm-1 h-1. Changes in the vertical profiles of the Argo data supported this partial freshening of salinity as well as the characteristic surface cooling and deepening of the mixed layer after the passage of the typhoon. We further demonstrated that the atmospheric environments in a rotated coordinate system along the typhoon paths showed clear salinity freshening in the forward and slightly left sides of the typhoon center. The spatial distinction of the wind and precipitation fields along the typhoon paths induced the characteristic synoptic response of salinity prior to the arrival of each typhoon. Our results provide reasonable observational evidence of oceanic responses to typhoons in the Northwest Pacific and contribute to the understanding of atmospheric and oceanic processes related to tropical storms.
Examining current best-practices for the use of wild post-larvae capture, culture, and release for fisheries enhancement
Authors: Richardson Laura E. , Lenfant Philippe , Clarke Leo J. , Fontcuberta Amelie , Gudefin Anaïs , Lecaillon Gilles , Le Vay Lewis , Radford Andrew N. , Simpson Stephen D.
Published: January 2023

Demand for marine fisheries is rising despite global impacts on the productive capacity of wild fish stocks due to overfishing, habitat loss, and global warming. Fisheries enhancement programs—aimed at augmenting stocks by releasing juveniles into the wild—are expected to play an increasingly important auxiliary role in addressing capture-based fishery limitations into the future. However, concerns exist over the impacts and efficacy of aquaculture-based enhancement (ABE), releasing captive-bred fish into wild populations. An alternative but understudied approach for fisheries enhancement is wild post-larvae capture, culture, and release (PCCR). Here, we provide an overview of the PCCR process, from initial planning to measuring success, providing an overview of its implementation in a viable finfish fishery, the white seabream Diplodus sargus in the Mediterranean. We discuss management application of PCCR-based enhancement and its limitations, highlighting existing knowledge gaps and future research required to realize the full potential of this alternative approach. Notwithstanding some limitations–including limited uptake for full evaluation, some species restrictions, density-dependent mortality, and the remaining open challenge for stock enhancement generally of tracking released fish through to reproduction–PCCR offers potential as a credible auxiliary management tool for fisheries restoration.
Optimization of environmental DNA analysis using pumped deep-sea water for the monitoring of fish biodiversity
Authors: Yoshida Takao , Kawato Masaru , Fujiwara Yoshihiro , Nagano Yuriko , Tsuchida Shinji , Yabuki Akinori
Published: January 2023

Deep-sea ecosystems present difficulties in surveying and continuous monitoring of the biodiversity of deep-sea ecosystems because of the logistical constraints, high cost, and limited opportunities for sampling. Environmental DNA (eDNA) metabarcoding analysis provides a useful method for estimating the biodiversity in aquatic ecosystems but has rarely been applied to the study of deep-sea fish communities. In this study, we utilized pumped deep-sea water for the continuous monitoring of deep-sea fish communities by eDNA metabarcoding. In order to develop an optimum method for continuous monitoring of deep-sea fish biodiversity by eDNA metabarcoding, we determined the appropriate amount of pumped deep-sea water to be filtered and the practical number of filtered sample replicates required for biodiversity monitoring of deep-sea fish communities. Pumped deep-sea water samples were filtered in various volumes (5–53 L) at two sites (Akazawa: pumping depth 800 m, and Yaizu: pumping depth 400 m, Shizuoka, Japan) of deep-sea water pumping facilities. Based on the result of evaluations of filtration time, efficiency of PCR amplification, and number of detected fish reads, the filtration of 20 L of pumped deep-sea water from Akazawa and filtration of 10 L from Yaizu were demonstrated to be suitable filtration volumes for the present study. Fish biodiversity obtained by the eDNA metabarcoding analyses showed a clear difference between the Akazawa and Yaizu samples. We also evaluated the effect of the number of filter replicates on the species richness detected by eDNA metabarcoding from the pumped deep-sea water. At both sites, more than 10 sample replicates were required for the detection of commonly occurring fish species. Our optimized method using pumped deep-sea water and eDNA metabarcoding can be applied to eDNA-based continuous biodiversity monitoring of deep-sea fish to better understand the effects of climate change on deep-sea ecosystems.
Reliability assessment of ultrasound muscle echogenicity in patients with rheumatic diseases: Results of a multicenter international web-based study
Authors: Di Matteo Andrea , Moscioni Erica , Lommano Maria Giovanna , Cipolletta Edoardo , Smerilli Gianluca , Farah Sonia , Airoldi Carla , Aydin Sibel Zehra , Becciolini Andrea , Bonfiglioli Karina , Carotti Marina , Carrara Greta , Cazenave Tomas , Corradini Davide , Cosatti Micaela Ana , de Agustin Juan Josè , Destro Castaniti Giulia Maria , Di Carlo Marco , Di Donato Eleonora , Di Geso Luca , Elliott Ashley , Fodor Daniela , Francioso Francesca , Gabba Alessandra , Hernández-Díaz Cristina , Horvath Rudolf , Hurnakova Jana , Jesus Diogo , Marin Josefina , Martire Maria Victoria , Mashadi Mirza Riccardo , Massarotti Marco , Musca Alice Andreea , Nair Jagdish , Okano Tadashi , Papalopoulos Ioannis , Rosa Javier , Rosemffet Marcos , Rovisco João , Rozza Davide , Salaffi Fausto , Scioscia Crescenzio , Scirè Carlo Alberto , Tamas Maria-Magdalena , Tanimura Shun , Ventura-Rios Lucio , Villota-Eraso Catalina , Villota Orlando , Voulgari Paraskevi V. , Vreju Florentin Ananu , Vukatana Gentiana , Hereter Johana Zacariaz , Zanetti Anna , Grassi Walter , Filippucci Emilio
Published: January 2023

Objectives

To investigate the inter/intra-reliability of ultrasound (US) muscle echogenicity in patients with rheumatic diseases.

Methods

Forty-two rheumatologists and 2 radiologists from 13 countries were asked to assess US muscle echogenicity of quadriceps muscle in 80 static images and 20 clips from 64 patients with different rheumatic diseases and 8 healthy subjects. Two visual scales were evaluated, a visual semi-quantitative scale (0–3) and a continuous quantitative measurement (“VAS echogenicity,” 0–100). The same assessment was repeated to calculate intra-observer reliability. US muscle echogenicity was also calculated by an independent research assistant using a software for the analysis of scientific images (ImageJ). Inter and intra reliabilities were assessed by means of prevalence-adjusted bias-adjusted Kappa (PABAK), intraclass correlation coefficient (ICC) and correlations through Kendall’s Tau and Pearson’s Rho coefficients.

Results

The semi-quantitative scale showed a moderate inter-reliability [PABAK = 0.58 (0.57–0.59)] and a substantial intra-reliability [PABAK = 0.71 (0.68–0.73)]. The lowest inter and intra-reliability results were obtained for the intermediate grades (i.e., grade 1 and 2) of the semi-quantitative scale. “VAS echogenicity” showed a high reliability both in the inter-observer [ICC = 0.80 (0.75–0.85)] and intra-observer [ICC = 0.88 (0.88–0.89)] evaluations. A substantial association was found between the participants assessment of the semi-quantitative scale and “VAS echogenicity” [ICC = 0.52 (0.50–0.54)]. The correlation between these two visual scales and ImageJ analysis was high (tau = 0.76 and rho = 0.89, respectively).

Conclusion

The results of this large, multicenter study highlighted the overall good inter and intra-reliability of the US assessment of muscle echogenicity in patients with different rheumatic diseases.
Trace element zinc and skin disorders
Authors: Zou Pan , Du Yuxin , Yang Chunguang , Cao Yuchun
Published: January 2023

Zinc is a necessary trace element and an important constituent of proteins and other biological molecules. It has many biological functions, including antioxidant, skin and mucous membrane integrity maintenance, and the promotion of various enzymatic and transcriptional responses. The skin contains the third most zinc in the organism. Zinc deficiency can lead to a range of skin diseases. Except for acrodermatitis enteropathic, a rare genetic zinc deficiency, it has also been reported in other diseases. In recent years, zinc supplementation has been widely used for various skin conditions, including infectious diseases (viral warts, genital herpes, cutaneous leishmaniasis, leprosy), inflammatory diseases (hidradenitis suppurativa, acne vulgaris, rosacea, eczematous dermatitis, seborrheic dermatitis, psoriasis, Behcet's disease, oral lichen planus), pigmentary diseases (vitiligo, melasma), tumor-associated diseases (basal cell carcinoma), endocrine and metabolic diseases (necrolytic migratory erythema, necrolytic acral erythema), hair diseases (alopecia), and so on. We reviewed the literature on zinc application in dermatology to provide references for better use.