bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a Numerical Investigation Framework for Ground Vehicle Platooning
Authors: Bounds Charles Patrick , Rajasekar Sudhan , Uddin Mesbah
Published: November 2021

This paper presents a study on the flow dynamics involving vehicle interactions. In order to do so, this study first explores aerodynamic prediction capabilities of popular turbulence models used in computational fluid dynamics simulations involving tandem objects and thus, ultimately presents a framework for CFD simulations of ground vehicle platooning using a realistic vehicle model, DrivAer. Considering the availability of experimental data, the simulation methodology is first developed using a tandem arrangement of surface-mounted cubes which requires an understanding on the role of turbulence models and the impacts of the associated turbulence model closure coefficients on the prediction veracity. It was observed that the prediction accuracy of the SST k−ω turbulence model can be significantly improved through the use of a combination of modified values for the closure coefficients. Additionally, the initial validation studies reveal the inability of the Unsteady Reynolds-Averaged Navier-Stokes (URANS) approach to resolve the far wake, and its frailty in simulating tandem body interactions. The Improved Delayed Detached Eddy Simulations (IDDES) approach can resolve the wakes with a reasonable accuracy. The validated simulation methodology is then applied to the fastback DrivAer model at different longitudinal spacing. The results show that, as the longitudinal spacing is reduced, the trailing car’s drag is increased while the leading car’s drag is decreased which supports prior explanations of vortex impingement as the reason for drag changes. Additionally, unlike the case of platooning involving Ahmed bodies, the trailing model drag does not return to an isolated state value at a two car-length separation. However, the impact of the resolution of the far wake of a detailed DrivAer model, and its implication on the CFD characterization of vehicle interaction aerodynamics need further investigations.
Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs
Authors: Liu Chujun , Guo Yawen , Wang Bo , Chen Lan , Xie Kaizhou , Yang Chenggen
Published: November 2021

A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm) with an external calibration method and electron impact (EI) ionization. Samples were pretreated using an accelerated solvent extraction (ASE) procedure followed by solid-phase extraction (SPE) on HLB cartridges (60 mg/3 mL). The derivative, which was safer and easier to store than penicillin G, was obtained by reacting trimethylsilyl diazomethane (TMSD) with penicillin G. The method was validated by the following parameters: linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The matrix-matched calibration curves had good linearity (R2 ≥ 0.9994) within the concentration range of LOQ–200.0 µg/kg for penicillin G in the sample matrices. In the same concentration range, the accuracy, in terms of recovery, was 80.31–94.50%; the relative standard deviation (RSD), intra-day RSD and inter-day RSD ranged from 1.24 to 3.44%, 2.13 to 4.82% and 2.74 to 6.13%, respectively. The LODs and LOQs of penicillin G in the matrices were in the ranges of 1.70–3.20 and 6.10–8.50 μg/kg, respectively. The applicability of the GC–MS/MS method was demonstrated by the determination of poultry eggs obtained from local markets with no penicillin G residues.
Local Linear Scale Factors in Map Projections of an Ellipsoid
Authors: Lapaine Miljenko
Published: November 2021

The main problem in cartography is that it is not possible to map/project/transform a spherical or ellipsoidal surface into a plane without distortions. The distortions of areas, angles, and/or distances are immanent to all maps. It is known that scale changes from point to point, and at certain points, the scale usually depends on the direction. The local linear scale factor c is one of the most important indicators of distortion distribution in the theory of map projections. It is not possible to find out the values of the local linear scale factor c in directions of coordinate axes x and y immediately from the definition of c. To solve this problem, in this paper, we derive new formulae for the calculation of c for a rotational ellipsoid. In addition, we derive the formula for computing c in any direction defined by dx and dy. We also considered the position and magnitude of the extreme values of c and derived new formulae for a rotational ellipsoid.
Factors Related to Oral Intake of Food by Hospitalized Patients with Malnutrition under the Care of a Nutrition Support Team
Authors: Furuya Junichi , Suzuki Hiroyuki , Hidaka Rena , Nakagawa Kazuharu , Yoshimi Kanako , Nakane Ayako , Yamaguchi Kohei , Shimizu Yukue , Itsui Yasuhiro , Saito Keiko , Sato Yuji , Tohara Haruka , Minakuchi Shunsuke
Published: November 2021

This study aimed to evaluate the role of the general condition and oral health status in determining the primary nutritional route and suitable food form for oral ingestion among malnourished inpatients. This cross-sectional study included 255 inpatients referred to a nutrition support team (NST), which included dental professionals, at an acute care hospital. We assessed the participants’ basic information, and Dysphagia Severity Scale (DSS) and Oral Health Assessment Tool (OHAT) scores. The nutritional intake mode was evaluated based on the Functional Oral Intake Scale scores at the initial NST consultation (FOIS-I), and then revised by the NST based on the participants’ general condition and oral health (FOIS-R). There was a divergence between FOIS-I and FOIS-R, with FOIS-R being significantly higher than FOIS-I (p < 0.001). Logistic regression analysis of FOIS-R identified that consciousness level (odds ratio (OR): 0.448; 95% confidence interval (CI): 0.214–0.935) and DSS (OR: 3.521; 95% CI: 2.574–4.815) significantly affected the oral nutrition intake. Among participants who could ingest orally (FOIS-R ≥ 3; n = 126), FOIS score had significant negative and positive associations with the OHAT and DSS scores, respectively. These findings suggest that appropriate assessment of oral health status, including swallowing function, might contribute to high-quality nutrition management.
Running Performance during the Holy Month of Ramadan in Elite Professional Adult Soccer Players in Russia
Authors: Bezuglov Eduard , Talibov Oleg , Khaitin Vladimir , Pirmakhanov Bekzhan , Waśkiewicz Zbigniew , Butovskiy Mikhail , Morgans Ryland
Published: November 2021

Religious fasting in the Holy Month of Ramadan is an important element of the Muslim culture during which no eating or drinking is permitted from dawn till dusk. A considerable number of Muslim soccer players abide by these restrictions, which may cause a negative impact on key running performance parameters during competitive matches. Alterations to diet and water intake during the Holy Month of Ramadan may affect various running performance parameters in elite Muslim professional adult soccer players. This study was conducted with two groups of soccer players from the Russian Premier League (RPL): The Exposure Group (EG) consisted of 13 Muslims age 24.0 ± 2.8 years abiding by religious fasting and the Control Group (CG) included 13 non-Muslim age 26.0 ± 4.4 years. Using the Instat system, the running performance of each player was controlled in both groups during matches from the RPL before and in the third week of Ramadan (a total of two matches for every player). None of the measured parameters demonstrated significant changes in any match. In conclusion, restrictions in diet and liquid intake during the Holy Month of Ramadan had no negative influence on the running performance of elite Muslim professional adult soccer players during daytime matches.
College Satisfaction, Sense of Achievement, Student Happiness and Sense of Belonging of Freshmen in Chinese Private Colleges: Mediation Effect of Emotion Regulation
Authors: Tian Jing , Zhang Mohan , Zhou Haitao , Wu Jianfen
Published: November 2021

Sense of belonging constitutes a critical component of college students’ retention and academic achievement, especially in disadvantaged higher education institutions such as private colleges in China. Using nationwide survey data (n = 3816) from Chinese private colleges, this study explores how college satisfaction, sense of achievement, and student happiness contribute to freshmen’ sense of belonging. Structural equation modeling analyses have identified the significant positive associations between freshmen’s emotion regulation and sense of belonging. In particular, emotion regulation plays a mediating role in the relations between college satisfaction, sense of achievement, student happiness, and the sense of belonging. Therefore, private colleges should design and implement orientation programs to improve freshmen’s learning experience, especially college satisfaction and student happiness, to enhance their sense of belonging.
Sports, Executive Functions and Academic Performance: A Comparison between Martial Arts, Team Sports, and Sedentary Children
Authors: Giordano Giulia , Gómez-López Manuel , Alesi Marianna
Published: November 2021

It is well known that curricular physical activity benefits children’s executive functions and academic performance. Therefore, this study aimed to determine whether there is an influence of extracurricular sports on executive functions and academic performance. However, it is less known which specific types of the sport better enhance executive functions in children; to investigate this issue, this study compared the performance on executive functions tasks and academic performance in one hundred and two boys and girls with an average age of 11.84 years recruited from Italian schools and gyms (N = 102), who participated in martial arts or team sports or were sedentary children. Executive functions were measured with the tests: Attenzione e Concentrazione, Digit Span test, Tower of London, IOWA Gambling task BVN 5-11, and BVN 12-18. Results demonstrated that children practicing martial arts showed better executive functioning and higher school marks than those involved in team sports or not involved in any sports. Furthermore, participants aged 12 to 15 years old outperformed in cool and hot executive functions tasks and had a better academic performance. Thus, the present findings supported the view that regular practice of extracurricular sports enhances executive functions development and consequently influences academic performance.
Association between Maternal Exposure to Chemicals during Pregnancy and the Risk of Foetal Death: The Japan Environment and Children’s Study
Authors: Ooka Tadao , Horiuchi Sayaka , Shinohara Ryoji , Kojima Reiji , Akiyama Yuka , Miyake Kunio , Otawa Sanae , Yokomichi Hiroshi , Yamagata Zentaro ,   on behalf of the Japan Environment and Children’s Study Group
Published: November 2021

Scarce knowledge is available on the relationship between maternal chemical exposure during pregnancy and foetal deaths. We studied the association of spontaneous abortions and stillbirths with occupational or daily maternal exposure to chemicals commonly used by pregnant women. Data from the Japan Environment and Children’s Study (JECS), a nationwide prospective birth cohort study, were used. The participants of the study were asked about the frequency of their use of gasoline, pesticides, hair dye, and chlorine bleach during the first and the second to third trimesters of pregnancy. We investigated the relationship between the frequency of the use of chemicals and foetal death. Of the 104,065 foetuses, 923 (0.91%) were spontaneous abortions and 379 (0.37%) were stillbirths. Any type of exposure during the first trimester was not significantly associated with spontaneous abortions. Nevertheless, a more than weekly occupational use of hair dye from the first to the second/third trimester was significantly associated with stillbirth. The results of this study suggest that the frequent use of hair dye during pregnancy can have severe adverse effects on the foetus. These findings can help pregnant women, especially hairdressers, refrain from the continuous use of hair dyes.
Estimating Economic Losses Caused by COVID-19 under Multiple Control Measure Scenarios with a Coupled Infectious Disease—Economic Model: A Case Study in Wuhan, China
Authors: Chen Xingtian , Gong Wei , Wu Xiaoxu , Zhao Wenwu
Published: November 2021

Background: The outbreak of the COVID-19 epidemic has caused an unprecedented public health crisis and drastically impacted the economy. The relationship between different control measures and economic losses becomes a research hotspot. Methods: In this study, the SEIR infectious disease model was revised and coupled with an economic model to quantify this nonlinear relationship in Wuhan. The control measures were parameterized into two factors: the effective number of daily contacts (people) (r); the average waiting time for quarantined patients (day) (g). Results: The parameter r has a threshold value that if r is less than 5 (people), the number of COVID-19 infected patients is very close to 0. A “central valley” around r = 5~6 can be observed, indicating an optimal control measure to reduce economic losses. A lower value of parameter g is beneficial to stop COVID-19 spread with a lower economic cost. Conclusion: The simulation results demonstrate that implementing strict control measures as early as possible can stop the spread of COVID-19 with a minimal economic impact. The quantitative assessment method in this study can be applied in other COVID-19 pandemic areas or countries.
Questionable Utility of the Eccentric Utilization Ratio in Relation to the Performance of Volleyball Players
Authors: Kozinc Žiga , Pleša Jernej , Šarabon Nejc
Published: November 2021

The difference between squat jump (SJ) and countermovement jump (CMJ), termed eccentric utilization ratio (EUR), is frequently suggested as an outcome that can be used in athletic training design. Unlike performance in SJ and CMJ, which is associated with sports performance, the association between EUR and sports performance is almost unexplored. This study aimed to investigate whether EUR is associated with performance in approach jump, linear sprint and change of direction (CoD) tasks in volleyball players. Forty-five male volleyball players performed SJ, CMJ, 25 m linear sprint, approach jump and two CoD tasks (505 test and modified T-test). EUR was calculated based on jump height, peak power, peak force and average power. SJ and CMJ variables showed moderate to high correlation with approach jump performance (r = 0.42–0.70), small correlation with modified T-test (r = 0.33–0.40) and small to moderate correlation with sprint time (r = 0.35–0.49). EUR showed only small associations with performance variables (r = 0.31–0.34). In all linear regression models with performance outcomes as dependent variables, the CMJ height was always the only statistically significant predictor. Our results support the recent arguments that the EUR offers limited insight into the neuromuscular capabilities of athletes.
Identification of DELLA Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
Authors: Guan Hongling , Huang Xinmin , Zhu Yunna , Xie Baoxing , Liu Houcheng , Song Shiwei , Hao Yanwei , Chen Riyuan
Published: November 2021

Flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) is an important and extensively cultivated vegetable in south China, and its stalk development is mainly regulated by gibberellin (GA). DELLA proteins negatively regulate GA signal transduction and may play an important role in determining bolting and flowering. Nevertheless, no systematic study of the DELLA gene family has been undertaken in flowering Chinese cabbage. In the present study, we found that the two-true-leaf spraying of gibberellin A3 (GA3) did not promote bolting but did promote flowering, whereas the three-true-leaf spraying of GA3 promoted both bolting and flowering. In addition, we identified five DELLA genes in flowering Chinese cabbage. All five proteins contained DELLA, VHYNP, VHIID, and SAW conserved domains. Protein-protein interaction results showed that in the presence of GA3, all five DELLA proteins interacted with BcGID1b (GA-INSENSITIVE DWARF 1b) but not with BcGID1a (GA-INSENSITIVE DWARF 1a) or BcGID1c (GA-INSENSITIVE DWARF 1c). Their expression analysis showed that the DELLA genes exhibited tissue-specific expression, and their reversible expression profiles responded to exogenous GA3 depending on the treatment stage. We also found that the DELLA genes showed distinct expression patterns in the two varieties of flowering Chinese cabbage. BcRGL1 may play a major role in the early bud differentiation process of different varieties, affecting bolting and flowering. Taken together, these results provide a theoretical basis for further dissecting the DELLA regulatory mechanism in the bolting and flowering of flowering Chinese cabbage.
Epigenetic Regulation of Endothelial Dysfunction and Inflammation in Pulmonary Arterial Hypertension
Authors: Hudson Jaylen , Farkas Laszlo
Published: November 2021

Once perceived as a disorder treated by vasodilation, pulmonary artery hypertension (PAH) has emerged as a pulmonary vascular disease with severe endothelial cell dysfunction. In the absence of a cure, many studies seek to understand the detailed mechanisms of EC regulation to potentially create more therapeutic options for PAH. Endothelial dysfunction is characterized by complex phenotypic changes including unchecked proliferation, apoptosis-resistance, enhanced inflammatory signaling and metabolic reprogramming. Recent studies have highlighted the role of epigenetic modifications leading to pro-inflammatory response pathways, endothelial dysfunction, and the progression of PAH. This review summarizes the existing literature on epigenetic mechanisms such as DNA methylation, histone modifications, and non-coding RNAs, which can lead to aberrant endothelial function. Our goal is to develop a conceptual framework for immune dysregulation and epigenetic changes in endothelial cells in the context of PAH. These studies as well as others may lead to advances in therapeutics to treat this devastating disease.
Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
Authors: Wójcik Barbara , Sawosz Ewa , Szczepaniak Jarosław , Strojny Barbara , Sosnowska Malwina , Daniluk Karolina , Zielińska-Górska Marlena , Bałaban Jaśmina , Chwalibog André , Wierzbicki Mateusz
Published: November 2021

Pancreatic cancer, due to its asymptomatic development and drug-resistance, is difficult to cure. As many metallic and carbon-based nanomaterials have shown anticancer properties, we decided to investigate their potential use as anticancer agents against human pancreatic adenocarcinoma. The objective of the study was to evaluate the toxic properties of the following nanomaterials: silver (Ag), gold (Au), platinum (Pt), graphene oxide (GO), diamond (ND), and fullerenol (C60(OH)40) against the cell lines BxPC-3, AsPC-1, HFFF-2, and HS-5. The potential cytotoxic properties were evaluated by the assessment of the cell morphology, cell viability, and cell membrane damage. The cancer cell responses to GO and ND were analysed by determination of changes in the levels of 40 different pro-inflammatory proteins. Our studies revealed that the highest cytotoxicity was obtained after the ND treatment. Moreover, BxPC-3 cells were more sensitive to ND than AsPC-1 cells due to the ND-induced ROS production. Furthermore, in both of the cancer cell lines, ND caused an increased level of IL-8 and a decreased level of TIMP-2, whereas GO caused only decreased levels of TIMP-2 and ICAM-1 proteins. This work provides important data on the toxicity of various nanoparticles against pancreatic adenocarcinoma cell lines.
Experimental Models, Induction Protocols, and Measured Parameters in Dry Eye Disease: Focusing on Practical Implications for Experimental Research
Authors: Rahman Md. Mahbubur , Kim Dong Hyun , Park Chul-Kyu , Kim Yong Ho
Published: November 2021

Dry eye disease (DED) is one of the major ophthalmological healthcare challenges worldwide. DED is a multifactorial disease characterized by a loss of homeostasis of the tear film, and its main pathogenesis is chronic ocular surface inflammation related with various cellular and molecular signaling cascades. The animal model is a reliable and effective tool for understanding the various pathological mechanisms and molecular cascades in DED. Considerable experimental research has focused on developing new strategies for the prevention and treatment of DED. Several experimental models of DED have been developed, and different animal species such as rats, mice, rabbits, dogs, and primates have been used for these models. Although the basic mechanisms of DED in animals are nearly identical to those in humans, proper knowledge about the induction of animal models is necessary to obtain better and more reliable results. Various experimental models (in vitro and in vivo DED models) were briefly discussed in this review, along with pathologic features, analytical approaches, and common measurements, which will help investigators to use the appropriate cell lines, animal, methods, and evaluation parameters depending on their study design.
Enterococcus faecium Regulates Honey Bee Developmental Genes
Authors: Du Yating , Luo Shiqi , Zhou Xin
Published: November 2021

Honey bees provide essential pollination services to the terrestrial ecosystem and produce important agricultural products. As a beneficial lactic acid bacterium, Enterococcus faecium is often supplied as a probiotic for honey bees and other animals. However, the underlying mechanisms of its actions and possible safety risks are not well understood. We present the first complete genome sequence of E. faecium isolated from the honey bee gut using nanopore sequencing, and investigate the effects and mechanisms of interactions between E. faecium and honey bees via transcriptome and miRNA analysis. E. faecium colonization increased honey bee gut weight. Transcriptome analysis showed that developmental genes were up-regulated. In accordance, the target genes of the down-regulated miRNAs were enriched in developmental pathways. We describe how E. faecium increases honey bee gut weight at the transcriptional and post-transcriptional levels, and add insights about how miRNAs mediate host and bacteria interactions.
Current Understanding of Temperature Stress-Responsive Chloroplast FtsH Metalloproteases
Authors: Luo Shengji , Kim Chanhong
Published: November 2021

Low and high temperatures are life-threatening stress factors, diminishing plant productivity. One of the earliest responses of plants to stress is a rapid burst of reactive oxygen species (ROS) in chloroplasts. Widespread efforts over the past decade shed new light on the chloroplast as an environmental sensor, translating the environmental fluctuation into varying physiological responses by utilizing distinct retrograde (chloroplast-to-nucleus) signals. Recent studies have unveiled that chloroplasts mediate a similar unfolded/misfolded/damaged protein response (cpUPR) as observed in the endoplasmic reticulum and mitochondria. Although observing cpUPR is not surprising since the chloroplast is a prime organelle producing harmful ROS, the intertwined relationship among ROS, protein damage, and chloroplast protein quality controls (cpPQCs) with retrograde signaling has recently been reported. This finding also gives rise to critical attention on chloroplast proteins involved in cpPQCs, ROS detoxifiers, transcription/translation, import of precursor proteins, and assembly/maturation, the deficiency of which compromises chloroplast protein homeostasis (proteostasis). Any perturbation in the protein may require readjustment of proteostasis by transmitting retrograde signal(s) to the nucleus, whose genome encodes most of the chloroplast proteins involved in proteostasis. This review focuses on recent findings on cpUPR and chloroplast-targeted FILAMENTOUS TEMPERATURE-SENSITIVE H proteases involved in cpPQC and retrograde signaling and their impacts on plant responses to temperature stress.
Alveologenesis: What Governs Secondary Septa Formation
Authors: Rippa Alexandra L. , Alpeeva Elena V. , Vasiliev Andrey V. , Vorotelyak Ekaterina A.
Published: November 2021

The simplification of alveoli leads to various lung pathologies such as bronchopulmonary dysplasia and emphysema. Deep insight into the process of emergence of the secondary septa during development and regeneration after pneumonectomy, and into the contribution of the drivers of alveologenesis and neo-alveolarization is required in an efficient search for therapeutic approaches. In this review, we describe the formation of the gas exchange units of the lung as a multifactorial process, which includes changes in the actomyosin cytoskeleton of alveocytes and myofibroblasts, elastogenesis, retinoic acid signaling, and the contribution of alveolar mesenchymal cells in secondary septation. Knowledge of the mechanistic context of alveologenesis remains incomplete. The characterization of the mechanisms that govern the emergence and depletion of αSMA will allow for an understanding of how the niche of fibroblasts is changing. Taking into account the intense studies that have been performed on the pool of lung mesenchymal cells, we present data on the typing of interstitial fibroblasts and their role in the formation and maintenance of alveoli. On the whole, when identifying cell subpopulations in lung mesenchyme, one has to consider the developmental context, the changing cellular functions, and the lability of gene signatures.
Emerging Anti-Atherosclerotic Therapies
Authors: Gluba-Brzózka Anna , Franczyk Beata , Rysz-Górzyńska Magdalena , Ławiński Janusz , Rysz Jacek
Published: November 2021

Cardiovascular disease (CAD) is the main cause of morbidity and deaths in the western world. The development of atherosclerosis underlying CAD development begins early in human life. There are numerous genetic and environmental risk factors accelerating its progression which then leads to the occurrence of acute events. Despite considerable progress in determining risk factors, there is still a lot of work ahead since identified determinants are responsible only for a part of overall CAD risk. Current therapies are insufficient to successfully reduce the risk of atherosclerosis development. Therefore, there is a need for effective preventive measures of clinical manifestations of atherosclerosis since the currently available drugs cannot prevent the occurrence of even 70% of clinical events. The shift of the target from lipid metabolism has opened the door to many new therapeutic targets. Currently, the majority of known targets for anti-atherosclerotic drugs focus also on inflammation (a common mediator of many risk factors), mechanisms of innate and adaptive immunity in atherosclerosis, molecule scavengers, etc. The therapeutic potential of cyclodextrins, protein kinase inhibitors, colchicine, inhibitors of p38 mitogen-activated protein kinase (MAPK), lipid dicarbonyl scavengers, a monoclonal antibody targeting interleukin-1β, and P-selectin inhibitors is still not fully confirmed and requires confirmation in large clinical trials. The preliminary results look promising.
Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from Cobitis taenia Complex
Authors: Dedukh Dmitrij , Marta Anatolie , Janko Karel
Published: November 2021

The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, how many gonial cells it affects and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: Cobitis) that reproduce by gynogenesis. We found that in naturally and experimentally produced F1 hybrids asexuality is achieved by genome endoreplication, which occurs in gonocytes just before entering meiosis or, rarely, one or a few divisions before meiosis. However, genome endoreplication was observed only in a minor fraction of the hybrid’s gonocytes, while the vast majority of gonocytes were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from natural clones than of experimentally produced F1 hybrids. Thus, asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with a possible impact on reproductive potential.
Mechanisms of Nucleosome Reorganization by PARP1
Authors: Maluchenko Natalya V. , Nilov Dmitry K. , Pushkarev Sergey V. , Kotova Elena Y. , Gerasimova Nadezhda S. , Kirpichnikov Mikhail P. , Langelier Marie-France , Pascal John M. , Akhtar Md. Sohail , Feofanov Alexey V. , Studitsky Vasily M.
Published: November 2021

Poly(ADP-ribose) polymerase 1 (PARP1) is an enzyme involved in DNA repair, chromatin organization and transcription. During transcription initiation, PARP1 interacts with gene promoters where it binds to nucleosomes, replaces linker histone H1 and participates in gene regulation. However, the mechanisms of PARP1-nucleosome interaction remain unknown. Here, using spFRET microscopy, molecular dynamics and biochemical approaches we identified several different PARP1-nucleosome complexes and two types of PARP1 binding to mononucleosomes: at DNA ends and end-independent. Two or three molecules of PARP1 can bind to a nucleosome depending on the presence of linker DNA and can induce reorganization of the entire nucleosome that is independent of catalytic activity of PARP1. Nucleosome reorganization depends upon binding of PARP1 to nucleosomal DNA, likely near the binding site of linker histone H1. The data suggest that PARP1 can induce the formation of an alternative nucleosome state that is likely involved in gene regulation and DNA repair.
An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress
Authors: Vernazza Stefania , Tirendi Sara , Passalacqua Mario , Piacente Francesco , Scarfì Sonia , Oddone Francesco , Bassi Anna Maria
Published: November 2021

Primary Open-Angle Glaucoma (POAG) is a neurodegenerative disease, and its clinical outcomes lead to visual field constriction and blindness. POAG’s etiology is very complex and its pathogenesis is mainly explained through both mechanical and vascular theories. The trabecular meshwork (TM), the most sensitive tissue of the eye anterior segment to oxidative stress (OS), is the main tissue involved in early-stage POAG, characterized by an increase in pressure. Preclinical assessments of neuroprotective drugs on animal models have not always shown correspondence with human clinical studies. In addition, intra-ocular pressure management after a glaucoma diagnosis does not always prevent blindness. Recently, we have been developing an innovative in vitro 3Dadvanced human trabecular cell model on a millifluidicplatform as a tool to improve glaucoma studies. Herein, we analyze the effects of prolonged increased pressure alone and, in association with OS, on such in vitro platform. Moreover, we verify whethersuch damaged TM triggers apoptosis on neuron-like cells. The preliminary results show that TM cells are less sensitive to pressure elevation than OS, and OS-damaging effects were worsened by the pressure increase. The stressed TM releases harmful signals, which increase apoptosis stimuli on neuron-like cells, suggesting its pivotal role in the glaucoma cascade.
Immediate Restoration of Single-Piece Zirconia Implants: A Prospective Case Series—Long-Term Results after 11 Years of Clinical Function
Authors: Steyer Elisabeth , Herber Valentin , Koller Martin , Végh Dániel , Mukaddam Khaled , Jakse Norbert , Payer Michael
Published: November 2021

Objectives: The aim of this prospective case series was to evaluate single-piece zirconia implants restored with lithium disilicate CAD/CAM crowns through a long-term follow-up. Methods: In this trial, 20 one-piece zirconia implants were placed in 20 patients. Implants were restored (i) immediately with lithium disilicate CAD/CAM provisionals, and (ii) permanently four months after surgery. Patients were followed for 11 years. Clinical parameters and radiological measurements of the zirconia implants were assessed. For the statistical analysis, paired t-test was applied. Results: Four implants were counted as implant failure due to the loss of implant stability, resulting in a Kaplan–Meier survival rate of 80% up to 11 years. The mean bleeding on probing values were 19.1% (SD ± 13.1) and 18.2% (SD ± 17.6) 96 and 11 years after implant placement, respectively. The plaque index revealed a significant decrease over time (p < 0.001) with a value between 25.9% (SD ± 5.7) and 12.6% (SD ± 10.0) at baseline and 11-years follow-up respectively. The marginal bone level revealed a significant decrease 4, 8, and 11 years after implant insertion (p = 0.001, p = 0.019, and p = 0.027, respectively). Conclusions: Immediately loaded zirconia single-piece implants showed a suitable success rate in clinical and radiographic outcomes.
Environmental Effects on Piezoelectric Sensors Array Signals and a Compensated Damage Imaging Method
Authors: Wang Zhiling , Zhong Yongteng , Zhou Jinyu , Li Chaoyue , Zhong Lina
Published: November 2021

Piezoelectric sensors array based damage imaging method as a high resolution source localization algorithm is becoming a promising method in structural health monitoring (SHM) technology. However, the environmental variations could affect the gain-phase of array signal. This paper experimentally evaluates the environmental effects on piezoelectric sensors array, and presents a compensated 2D-MUSIC based damage imaging method for composite structures. Firstly, detailed analysis and comparison discussion about the gain-phase difference of array signal when the environmental parameters change, and the gain-phase changes respect to the environmental parameters could be obtained. Secondly, array error matrix is structured and substituted into the steering vector of the original 2D-MUSIC algorithm to compensate. Finally, the compensated 2D-MUSIC algorithm is applied for estimating the initial estimates of damage. After substituting these initial estimates, the cost function is minimized by adaptive iterative calculating the reasonable location of the damage source. The experiments on an epoxy laminate plate demonstrate the validity and effectiveness of the proposed method.
Demultiplexer of Multi-Order Correlation Interference in Nitrogen Vacancy Center Diamond
Authors: Li Xinghua , Raza Faizan , Li Yufeng , Wang Jinnan , Wang Jinhao , Ali Hasnain , Wang Luyuan , Zhao Yuan , Zhang Yanpeng
Published: November 2021

We reported the second- and third-order temporal interference of two non-degenerate pseudo-thermal sources in a nitrogen-vacancy center (NV−). The relationship between the indistinguishability of source and path alternatives is analyzed at low temperature. In this article, we demonstrate the switching between three-mode bunching and frequency beating effect controlled by the time offset and the frequency difference to realize optical demultiplexer. Our experimental results suggest the advanced technique achieves channel spacing and speed of the demultiplexer of about 96% and 17 ns, respectively. The proposed demultiplexer model will have potential applications in quantum computing and communication.
High Aspect Ratio Structuring of Glass with Ultrafast Bessel Beams
Authors: Vetter Christian , Giust Remo , Furfaro Luca , Billet Cyril , Froehly Luc , Courvoisier Francois
Published: November 2021

Controlling the formation of high aspect ratio void channels inside glass is important for applications like the high-speed dicing of glass. Here, we investigate void formation using ultrafast Bessel beams in the single shot illumination regime. We characterize the morphology of the damages as a function of pulse energy, pulse duration, and position of the beam inside fused silica, Corning Eagle XG, and Corning Gorilla glass. While a large set of parameters allow for void formation inside fused silica, the operating window is much more restricted for Eagle XG and Gorilla glass. The transient formation of a molten layer around voids enables us interpreting the evolution of the morphology with pulse energy and duration.
Microstructure Evolution and a Unified Constitutive Model of Ti-55511 Alloy Compressed at Stepped Strain Rates
Authors: Su Gang , Yun Zhong , Lin Yong-Cheng , He Dao-Guang , Zhang Song , Chen Zi-Jian
Published: November 2021

The flow behavior and microstructure change of the Ti-55511 alloy are investigated by thermal compression experiments with stepped strain rates. The phase transformation features, the dynamic recrystallization (DRX) behavior of the β matrix, the dynamic spheroidization mechanism of the lamellar α phase and the evolution of the β sub-grain size are quantitatively analyzed. A unified constitutive model is constructed to characterize the hot deformation features of the Ti-55511 alloy. In the established model, the work hardening effect is taken into account by involving the coupled effects of the equiaxed and lamellar α phases, as well as β substructures. The dynamic softening mechanisms including the dynamic recovery (DRV), DRX and dynamic spheroidization mechanisms are also considered. The material parameters are optimized by the multi-objective algorithm in the MATLAB toolbox. The consistency between the predicted and experimental data indicates that the developed unified model can accurately describe the flow features and microstructure evolution of the hot compressed Ti-55511 at stepped strain rates.
Laboratory Tests of Concrete Beams Reinforced with Recycled Steel Fibres and Steel Bars
Authors: Pająk Małgorzata , Wandzik Grzegorz
Published: November 2021

This paper explores the possibility of the partial replacement of the longitudinal reinforcement in reinforced concrete (RC) beams with recycled steel fibres (RSF). Testing was focused on the contribution of two volume ratios of the RSF—0.5%, 1.0%. Basic compression and flexural tensile tests were performed to evaluate the effectiveness of the fibres following current standards. Additionally, the full-scale beams with and without conventional reinforcement were subjected to four-point bending tests. The results indicate that RSF improved the load-bearing capacity of the RC beams. Cooperation of RSF with the steel bars in carrying loads was proved. Findings from the Digital Image Correlation (DIC) revealed no impact on the cracking pattern of the RC beams.
A Novel Fabricating Method of Micro Lens-on-Lens Arrays with Two Focal Lengths
Authors: Liu Xin , Li Min , Bian Jiang , Du Junfeng , Li Bincheng , Fan Bin
Published: November 2021

Micro lens-on-lens array (MLLA) is a novel 3D structure with unique optical properties that cannot be fabricated accurately and quickly by existing processing methods. In this paper, a new fabricating method of MLLAs with two focal lengths is proposed. By introducing the soft lithography technology, nano-imprint technology and mask alignment exposure technology, MLLAs with high precisions can be obtained. A MLLA is successfully fabricated with two focal lengths of 58 μm and 344 μm, and an experiment is carried out. The results show that the MLLA has excellent two-level focusing and imaging abilities. Furthermore, the fabricated profiles of the MLLA agree well with the designed profiles, and the morphology deviation of the MLLA is better than 2%, satisfying the application requirements. The results verify the feasibility and validity of the novel fabricating method. By adjusting mask patterns and processing parameters, MLLAs with both changeable sizes and focal lengths can be obtained.
Uranium (VI) Adsorbate Structures on Portlandite [Ca(OH)2] Type Surfaces Determined by Computational Modelling and X-ray Absorption Spectroscopy
Authors: Lee Christopher A. , van Veelen Arjen , Morris Katherine , Mosselmans J. Fred W. , Wogelius Roy A. , Burton Neil A.
Published: November 2021

Portlandite [Ca(OH)2] is a potentially dominant solid phase in the high pH fluids expected within the cementitious engineered barriers of Geological Disposal Facilities (GDF). This study combined X-ray Absorption Spectroscopy with computational modelling in order to provide atomic-scale data which improves our understanding of how a critically important radionuclide (U) will be adsorbed onto this phase under conditions relevant to a GDF environment. Such data are fundamental for predicting radionuclide mass transfer. Surface coordination chemistry and speciation of uranium with portlandite [Ca(OH)2] under alkaline groundwater conditions (ca. pH 12) were determined by both in situ and ex situ grazing incidence extended X-ray absorption fine structure analysis (EXAFS) and by computational modelling at the atomic level. Free energies of sorption of aqueous uranyl hydroxides, [UO2(OH)n]2–n (n = 0–5) with the (001), (100) and (203) or (101) surfaces of portlandite are predicted from the potential of mean force using classical molecular umbrella sampling simulation methods and the structural interactions are further explored using fully periodic density functional theory computations. Although uranyl is predicted to only weakly adsorb to the (001) and (100) clean surfaces, there should be significantly stronger interactions with the (203/101) surface or at hydroxyl vacancies, both prevalent under groundwater conditions. The uranyl surface complex is typically found to include four equatorially coordinated hydroxyl ligands, forming an inner-sphere sorbate by direct interaction of a uranyl oxygen with surface calcium ions in both the (001) and (203/101) cases. In contrast, on the (100) surface, uranyl is sorbed with its axis more parallel to the surface plane. The EXAFS data are largely consistent with a surface structural layer or film similar to calcium uranate, but also show distinct uranyl characteristics, with the uranyl ion exhibiting the classic dioxygenyl oxygens at 1.8 Å and between four and five equatorial oxygen atoms at distances between 2.28 and 2.35 Å from the central U absorber. These experimental data are wholly consistent with the adsorbate configuration predicted by the computational models. These findings suggest that, under the strongly alkaline conditions of a cementitious backfill engineered barrier, there would be significant uptake of uranyl by portlandite to inhibit the mobility of U(VI) from the near field of a geological disposal facility.
Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
Authors: Kongkiatkamon Suchada , Ramachandran Amsaveni , Knoernschild Kent L. , Campbell Stephen D. , Sukotjo Cortino , George Anne
Published: November 2021

Dentin matrix protein 1 (DMP1) contains a large number of acidic domains, multiple phosphorylation sites, a functional arginine-glycine-aspartate (RGD) motif, and a DNA binding domain, and has been shown to play essential regulatory function in dentin and bone mineralization. DMP1 could also orchestrate bone matrix formation, but the ability of DMP1 on Ti to human mesenchymal stem cell (hMSC) conversion to osteoblasts has not been studied. There is importance to test if the DMP1 coated Ti surface would promote cell migration and attachment to the metal surface and promote the differentiation of the attached stem cells to an osteogenic lineage. This study aimed to study the human mesenchymal stem cells (hMSCs) attachment and proliferation on DMP1 coated titanium (Ti) disks compared to non-coated disks, and to assess possible osteoblastic differentiation of attached hMSCs. Sixty-eight Ti disks were divided into two groups. Group 1 disks were coated with dentin matrix protein 1 and group 2 disks served as control. Assessment with light microscopy was used to verify hMSC attachment and proliferation. Cell viability was confirmed through fluorescence microscopy and mitochondrial dehydrogenase activity. Real-time polymerase chain reaction analysis was done to study the gene expression. The proliferation assay showed significantly greater cell proliferation with DMP1 coated disks compared to the control group (p-value < 0.001). Cell vitality analysis showed a greater density of live cells on DMP1 coated disks compared to the control group. Alkaline phosphatase staining revealed higher enzyme activity on DMP1 coated disks and showed itself to be significantly higher than the control group (p-value < 0.001). von Kossa staining revealed higher positive areas for mineralized deposits on DMP1 coated disks than the control group (p-value < 0.05). Gene expression analysis confirmed upregulation of runt-related transcription factor 2, osteoprotegerin, osteocalcin, osteopontin, and alkaline phosphatase on DMP1 coated disks (p-value < 0.001). The dentin matrix protein promoted the adhesion, proliferation, facilitation differentiation of hMSC, and mineralized matrix formation.
Bipolar Resistive Switching Behavior of PVP-GQD/HfOx/ITO/Graphene Hybrid Flexible Resistive Random Access Memory
Authors: Kim Jin Mo , Hwang Sung Won
Published: November 2021

We have investigated highly flexible memristive devices using reduced graphene oxide (RGO) nanosheet nanocomposites with an embedded GQD Layer. Resistive switching behavior of poly (4-vinylphenol):graphene quantum dot (PVP:GQD) composite and HfOx hybrid bilayer was explored for developing flexible resistive random access memory (RRAM) devices. A composite active layer was designed based on graphene quantum dots, which is a low-dimensional structure, and a heterogeneous active layer of graphene quantum dots was applied to the interfacial defect structure to overcome the limitations. Increasing to 0.3–0.6 wt % PVP-GQD, Vf changed from 2.27–2.74 V. When negative deflection is applied to the lower electrode, electrons travel through the HfOx/ITO interface. In addition, as the PVP-GQD concentration increased, the depth of the interfacial defect decreased, and confirmed the repetition of appropriate electrical properties through Al and HfOx/ITO. The low interfacial defects help electrophoresis of Al+ ions to the PVP GQD layer and the HfOx thin film. A local electric field increase occurred, resulting in the breakage of the conductive filament in the defect.