bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation on the impact of quality characteristics and storage stability of foxtail millet induced by air cold plasma
Authors: Wang Lang-Hong , Li Zhongyan , Qin Jiale , Huang Yanyan , Zeng Xin-An , Aadil Rana Muhammad
Published: December 2022

The aim of this work was to investigate the effects of dielectric barrier discharge-air cold plasma (DBD-ACP, 15–35 kV, 2–12 min) on the quality of foxtail millets. The L and b* values were evaluated by a digital colorimeter representing that the color of millets was significantly changed at 25 kV for 4–12 min or at 35 kV for 2–12 min. The results were consistent with the change of total yellow pigment in millets, indicating that DBD-ACP damaged the carotenoids if the treatment condition was too high. The activity of lipoxygenase and lipase, involving the oxidation and hydrolysis of lipids of millet, decreased significantly induced by DBD-ACP. For example, the lipoxygenase and lipase activity of Mizhi millet was decreased from 44.0 to 18.7 U g–1min–1, 56.0–15.1 U/(mg pro) (p<0.05) after being exposed to 25 kV for 2–12 min, respectively. Changes of color, lipoxygenase and lipase activity, and malondialdehyde content of millets were determined during accelerated storage (40 ± 2°C and 75% Relative Humidity) for 15 days after being treated by DBD-ACP under 15 and 25 kV for 4 min. Results showed that millets treated by DBD-ACP at 15 kV kept a better color with lower malondialdehyde content, and lower lipoxygenase and lipase activity compared to control. This work implied that DBD-ACP is an underlying approach for the storage of foxtail millets.
18F-PSMA-1007 PET/CT-derived semi-quantitative parameters for risk stratification of newly diagnosed prostate cancer
Authors: Dong Siying , Li Yanmei , Chen Jian , Li Yongliang , Yang Pengfei , Li Juan
Published: December 2022

Purpose

This study aimed to assess the value of 18F-PSMA-1007 positron emission tomography/computed tomography (PET/CT)-derived semi-quantitative parameters of primary tumor for risk stratification of newly diagnosed prostate cancer (PCa).

Methods

Sixty patients referred for 18F-PSMA-1007 PET/CT imaging for primary PCa were retrospectively analyzed and classified into the low-intermediate-risk (LIR) or high-risk (HR) group. The maximum standardized uptake value (SUVmax) of primary tumor, prostate total lesion PSMA (TL-PSMAp), and prostate PSMA-tumor volume (PSMA-TVp) were measured, and group differences were evaluated using the Mann–Whitney U test. Spearman’s correlation was performed to assess the correlation between the above parameters with prostate-specific antigen (PSA) levels and Gleason score (GS). Receiver operating characteristic (ROC) curve analysis was used to determine optimal cut-off values for SUVmax, TL-PSMAp, and PSMA-TVp to identify high-risk PCa and compare diagnostic efficacy.

Results

Among 60 patients, 46 were assigned to the HR group and 16 to the LIR group. In all patients, SUVmax, TL-PSMAp, and PSMA-TVp were moderately correlated with pre-treatment PSA values (r = 0.411, p = 0.001; r = 0.663, p < 0.001; and r = 0.549, p < 0.001, respectively). SUVmax and TL-PSMAp were moderately correlated with GS (r = 0.457 and r = 0.448, respectively; p < 0.001), while PSMA-TVp was weakly correlated with GS (r = 0.285, p = 0.027). In the ROC curve analysis, the optimal cut-off values of SUVmax, TL-PSMAp, and PSMA-TVp for identifying high-risk PCa were 9.61, 59.62, and 10.27, respectively, and the areas under the operating curve were 0.828, 0.901, and 0.809, respectively. The sensitivities of SUVmax, TL-PSMAp, and PSMA-TVp were 91.03%, 71.74%, and 63.04%, respectively, and the specificities were 71.43%, 100.00%, and 92.86%, respectively.

Conclusions

TL-PSMAp had a superior ability to identify high-risk PCa. The semi-quantitative parameters of primary tumor on 18F-PSMA-1007 PET/CT imaging can be an objective imaging reference index to determine PCa risk stratification.
DEPDC1B promotes development of cholangiocarcinoma through enhancing the stability of CDK1 and regulating malignant phenotypes
Authors: Zhang Zhenhai , Wang Xinxing , Nie Peihua , Qin Yejun , Shi Junping , Xu Shifeng
Published: December 2022

Cholangiocarcinoma (CCA) is the second most common primary tumor of the hepatobiliary system. At present, the therapeutic efficiency of cholangiocarcinoma is fairly low and the prognosis is poor. The root cause is that the molecular mechanism of the occurrence and development of CCA is largely unclear. This work intended to clarify the role of DEP domain-containing protein 1B (DEPDC1B) in the progress of CCA through cellular biology research strategies and further clarify the molecular mechanism of CCA. Clinical tissue-related detection showed that the expression level of DEPDC1B in tumor tissues was significantly higher than that in normal tissues and was positively correlated with tumor grade. Knockdown of the endogenous DEPDC1B of CCA cells can significantly inhibit cell proliferation and migration, while promoting cell apoptosis and blocking the cell cycle. DEPDC1B overexpression induced the opposite effects. Studies in animal models also showed that the downregulation of DEPDC1B can reduce the tumorigenicity of CCA cells. In addition, through gene profiling analysis and molecular biology studies, we found that CDK1 may be an important downstream mediator of DEPDC1B, the protein stability of which was significantly decreased through the ubiquitin–proteasome system in DEPDC1B knockdown cells. Moreover, knockdown of CDK1 can weaken the promotion of CCA caused by DEPDC1B overexpression. In summary, our research showed that DEPDC1B plays an important role in the development of CCA and its targeted inhibition may become one of the important methods to inhibit the progress of CCA.
Breast tumor IGF1R regulates cell adhesion and metastasis: alignment of mouse single cell and human breast cancer transcriptomics
Authors: Obr Alison E. , Bulatowicz Joseph J. , Chang Yun-Juan , Ciliento Virginia , Lemenze Alexander , Maingrette Krystopher , Shang Quan , Gallagher Emily J. , LeRoith Derek , Wood Teresa L.
Published: December 2022

Introduction

The acquisition of a metastatic phenotype is the critical event that determines patient survival from breast cancer. Several receptor tyrosine kinases have functions both in promoting and inhibiting metastasis in breast tumors. Although the insulin-like growth factor 1 receptor (IGF1R) has been considered a target for inhibition in breast cancer, low levels of IGF1R expression are associated with worse overall patient survival.

Methods

To determine how reduced IGF1R impacts tumor phenotype in human breast cancers, we used weighted gene co-expression network analysis (WGCNA) of Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) patient data to identify gene modules associated with low IGF1R expression. We then compared these modules to single cell gene expression analyses and phenotypes of mouse mammary tumors with reduced IGF1R signaling or expression in a tumor model of triple negative breast cancer.

Results

WGCNA from METABRIC data revealed gene modules specific to cell cycle, adhesion, and immune cell signaling that were inversely correlated with IGF1R expression in human breast cancers. Integration of human patient data with single cell sequencing data from mouse tumors revealed similar pathways necessary for promoting metastasis in basal-like mammary tumors with reduced signaling or expression of IGF1R. Functional analyses revealed the basis for the enhanced metastatic phenotype including alterations in E- and P-cadherins.

Discussion

Human breast and mouse mammary tumors with reduced IGF1R are associated with upregulation of several pathways necessary for promoting metastasis supporting the conclusion that IGF1R normally helps maintain a metastasis suppressive tumor microenvironment. We further found that reduced IGF1R signaling in tumor epithelial cells dysregulates cadherin expression resulting in reduced cell adhesion.
Neonatal AKI profile using KDIGO guidelines: A cohort study in tertiary care hospital ICU of Lahore, Pakistan
Authors: Gul Rafia , Anwar Zahid , Sheikh Mehmood , Salamat Ayesha , Iqbal Samer , Saleem Furqan , Fatima Samer
Published: December 2022

Background and objective

Acute kidney injury (AKI) was observed in sick neonates and was associated with poor outcomes. Our cohort represents the neonatal characteristics of those diagnosed with AKI using Kidney Disease: Improved Global Outcome (KDIGO) guidelines.

Methodology

A cohort study was conducted in the NICU of FMH from June 2019 to May 2021. Data were collected on a proforma. All continuous variables were not normally distributed and expressed as the median and interquartile range. Categorical variables were analyzed by proportional differences with the Pearson chi-square test or Fisher's exact tests. A multinomial logistic regression model was used to explore the independent risk factors for AKI. Time to the event (death) and the cohort's survival curves were plotted using the Cox proportional hazard model.

Results

AKI occurred in 473 (37.6%) neonates. The risk factors of AKI were outborn birth [adjusted odds ratio (AOR): 3.987, 95% confidence interval (CI): 2.564–6.200, p: 0.000], birth asphyxia (AOR: 3.567, 95% CI: 2.093–6.080, p: 0.000), inotropic agent (AOR: 2.060, 95% CI: 1.436–2.957, p: 0.000), antenatal steroids (AOR: 1.721, 95% CI: 1.213–2.443, p: 0.002), central lines (AOR: 1.630, 95% CI: 1.155–2.298, p: 0.005) and intraventricular hemorrhage (IVH)/intracranial hemorrhage/disseminated intravascular coagulopathy (AOR: 1.580, 95% CI: 1.119–2.231, p: 0.009). AKI significantly increases the duration of stay and mortality rates by 16.5% vs. 3.9% in neonates with normal renal function (p < 0.001).

Conclusion

About one-third of critically sick neonates had AKI. Significant risk factors for AKI were outborn birth, asphyxia inotropic agents, necrotizing enterocolitis, antenatal steroids central lines, and IVH. AKI is associated with an increased length of stay and increased mortality.
A nomogram for predicting upper urinary tract damage risk in children with neurogenic bladder
Authors: Li Qi , Cai Miao , Pu Qingsong , Wu Shengde , Liu Xing , Lin Tao , He Dawei , Wen Jianguo , Wei Guanghui
Published: December 2022

Purpose

To establish a predictive model for upper urinary tract damage (UUTD) in children with neurogenic bladder (NB) and verify its efficacy.

Methods

A retrospective study was conducted that consisted of a training cohort with 167 NB patients and a validation cohort with 100 NB children. The clinical data of the two groups were compared first, and then univariate and multivariate logistic regression analyses were performed on the training cohort to identify predictors and develop the nomogram. The accuracy and clinical usefulness of the nomogram were verified by receiver operating characteristic (ROC) curve, calibration curve and decision curve analyses.

Results

There were no significant differences in other parameters between the training and validation cohorts except for age (all P > 0.05). Recurrent urinary tract infection, bladder compliance, detrusor leak point pressure, overactive bladder and clean intermittent catheterization were identified as predictors and assembled into the nomogram. The nomogram showed good discrimination with the area under the ROC curve (AUC) in the training cohort (0.806, 95% CI: 0.737–0.874) and validation cohort (0.831, 95% CI: 0.753–0.0.909). The calibration curve showed that the nomograms were well calibrated, with no significant difference between the predicted and observed probabilities. Decision curve analysis indicated that the nomogram has good clinical applicability.

Conclusion

This study presents an effective nomogram incorporating five clinical characteristics that can be conveniently applied to assess NB children' risk of progressing to UUTD.
Post-transplant lymphoproliferative disease after pediatric kidney transplant
Authors: Fulchiero Rosanna , Amaral Sandra
Published: December 2022

Post-transplant lymphoproliferative disease (PTLD) is the most common malignancy complicating solid organ transplantation (SOT) in adults and children. PTLD encompasses a spectrum of histopathologic features and organ involvement, ranging from benign lymphoproliferation and infectious-mononucleosis like presentation to invasive neoplastic processes such as classical Hodgkin lymphoma. The predominant risk factors for PTLD are Epstein-Barr virus (EBV) serostatus at the time of transplant and the intensity of immunosuppression following transplantation; with EBV-negative recipients of EBV-positive donor organs at the highest risk. In children, PTLD commonly presents in the first two years after transplant, with 80% of cases in the first year, and over 90% of cases associated with EBV-positive B-cell proliferation. Though pediatric kidney transplant recipients are at lower risk (1–3%) for PTLD compared to their other SOT counterparts, there is still a significant risk of morbidity, allograft failure, and an estimated 5-year mortality rate of up to 50%. In spite of this, there is no consensus for monitoring of at-risk patients or optimal management strategies for pediatric patients with PTLD. Here we review pathogenesis and risk factors for the development of PTLD, with current practices for prevention, diagnosis, and management of PTLD in pediatric kidney transplant recipients. We also highlight emerging concepts, current research gaps and potential future developments to improve clinical outcomes and longevity in these patients.
Underlying beneficial effects of Rhubarb on constipation-induced inflammation, disorder of gut microbiome and metabolism
Authors: Gao Han , He Chengwei , Hua Rongxuan , Liang Chen , Wang Boya , Du Yixuan , Xin Shuzi , Guo Yuexin , Gao Lei , Zhang Lucia , Shang Hongwei , Xu Jingdong
Published: December 2022

Graphical Abstract

1. Constipation leads to the enhanced thickness of muscle layer and sedimentation of collagenous fiber. 2. Constipation promotes not only the pro-inflammtory cytokines secretion but also damage the integrity of intestinal barrier. 3. The present results are significant in at least two major respects in the alternations of the diversity of gut microbiome along with the fluctuation of the fatty acid and bioamine in constipation mice. 4. One unanticipated finding is that the rhubarb extract has the potential to reverse the alterations induced by constipation.
Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment
Authors: Xue Xin-miao , Liu Yu-yang , Chen Xue-min , Tao Bing-yan , Liu Peng , Zhou Han-wen , Zhang Chi , Wang Li , Jiang Yu-ke , Ding Zhi-wei , Shen Wei-dong , Zhang Jun , Yang Shi-ming , Wang Fang-yuan
Published: December 2022

Background: Ecto-5′-nucleotidase (NT5E) encodes the cluster of differentiation 73 (CD73), whose overexpression contributes to the formation of immunosuppressive tumor microenvironment and is related to exacerbated prognosis, increased risk of metastasis and resistance to immunotherapy of various tumors. However, the prognostic significance of NT5E in pan-cancer is obscure so far.

Methods: We explored the expression level of NT5E in cancers and adjacent tissues and revealed the relationship between the NT5E expression level and clinical outcomes in pan-cancer by utilizing the UCSC Xena database. Then, correlation analyses were performed to evaluate the relationship between NT5E expression and immune infiltration level via EPIC, MCP-counter and CIBERSORT methods, and the enrichment analysis were employed to identify NT5E-interacting molecules and functional pathways. Furthermore, we conducted single-cell analysis to explore the potential role of NT5E on single-cell level based on the CancerSEA database. Meanwhile, gene set enrichment analysis (GSEA) in single-cell level was also conducted in TISCH database and single-cell signature explorer was utilized to evaluate the epithelial-mesenchymal transition (EMT) level in each cell type.

Results: The expression level of NT5E was aberrant in almost all cancer types, and was correlated with worse prognosis in several cancers. Notably, NT5E overexpression was related to worse overall survival (OS) in pancreatic adenocarcinoma (PAAD), head and neck squamous cell carcinoma (HNSC), mesothelioma (MESO), stomach adenocarcinoma (STAD), uveal melanoma (UVM) and cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) (p < 0.01). NT5E-related immune microenvironment analysis revealed that NT5E is associated positively with the degree of infiltration of cancer-associated fibroblasts (CAFs) and endothelial cells in most cancers. Enrichment analysis of cellular component (CC) demonstrated the critical part of NT5E played in cell-substrate junction, cell-substrate adherens junction, focal adhesion and external side of plasma membrane. Finally, single-cell analysis of NT5E illuminated that EMT function of CAFs was elevated in basal cell carcinoma (BCC), skin cutaneous melanoma (SKCM), HNSC and PAAD.

Conclusion: NT5E could serve as a potential prognostic biomarker for cancers. The potential mechanism may be related to the upregulated EMT function of CAFs, which provides novel inspiration for immunotherapy by targeting CAFs with high NT5E expression.
A numerical analysis of the transport of modified hybrid nanofluids containing various nanoparticles with mixed convection applications in a vertical cylinder
Authors: Riaz Arshad , Abbasi A. , Al-Khaled Kamel , Gulzar Sidra , Khan Sami Ullah , Farooq W. , El-Din ElSayed M. Tag
Published: December 2022

The hybrid materials are an impressive class of nanofluids with exciting thermal outcomes and present applications in enhancing the heat transfer procedure, solar energy, extrusion processes, and in different engineering processes. The current contribution aims to reflect the improved mechanism of the heat transfer phenomenon for hybrid nanofluids. Aluminum oxide, copper, and copper oxide at different solid volume fractions are used to report the thermal phenomenon. For the base material, water is used. The mixed convection applications are also encountered. The moving cylinder with a stretched uniform velocity causes the flow. The velocity slip and convective boundary constraints are used to observe the flow phenomenon. The hybrid nanofluid is expressed via different mathematical relations. The shape factors for hybrid nanomaterials are presented. The Keller box numerical method with effective accuracy has been entertained for the simulation process. The applications of parameters for the current model are explained via graphs.
A three-dimension terminal simulation tool based on transfinite element method
Authors: Yang Siyi , Wang Hao , Xu Li , Chen Muyi , Liu Bingqi , Liu Hangxin , Li Bin
Published: December 2022

In the design of high-speed interconnection systems, it is often necessary to evaluate signal integrity and electromagnetic interference performance by extracting terminal network parameters and differential and common mode network parameters. However, in a multi-conductor transmission line, the characteristic parameters related to different conductors, which named terminal in terminal theory, are couped together in traditional model network parameters and could not be extracted directly. Therefore, in this paper, a three-dimension terminal simulation tool based on transfinite element method is developed to solve this problem. The vector finite element method is combined with the transfinite element method, and the electric field of the truncation surface is approximated by the expansion of the truncation surface mode function. As far as we know, the combination of the transfinite element method and the terminal theory is proposed for the first time. The terminal simulation tool has a human-computer interaction interface, as well as functions such as modeling, mesh generation, solution setting, and post-processing result display, which can accurately obtain the terminal network parameters and differential and common mode network parameters. Numerical examples are given to demonstrate the accuracy and efficiency of the developed tool compared with the results from HFSS.
Correlations of strength, proprioception, and tactile sensation to return-to-sports readiness among patients with anterior cruciate ligament reconstruction
Authors: Ma Xiaoli , Lu Lintao , Zhou Zhipeng , Sun Wei , Chen Yan , Dai Guofeng , Wang Cheng , Ding Lijie , Fong Daniel Tik-Pui , Song Qipeng
Published: December 2022

Objectives: Anterior cruciate ligament reconstruction (ACLR) is the most common surgery for anterior cruciate ligament (ACL) injuries, and the relationships between patients’ return to sports (RTS) readiness and different physical functions are inconclusive among patients with ACLR. This study aimed to investigate the correlations of strength, proprioception, and tactile sensation to the RTS readiness among patients with ACLR.

Methods: Forty-two participants who received ACLR for at least 6 months were enrolled in this study. Their strength, proprioception, and tactile sensation were tested, and their RTS readiness was measured with the Knee Santy Athletic Return to Sports (K-STARTS) test, which consists of a psychological scale [Anterior Cruciate Ligament Return to Sports after Injury scale (ACL-RSI)] and seven functional tests. Partial correlations were used to determine their correlations while controlling for covariates (age, height, weight, and postoperative duration), and factor analysis and multivariable linear regressions were used to determine the degrees of correlation.

Results: Knee extension strength was moderately correlated with K-STARTS total, ACL-RSI, and functional scores. Knee flexion strength, knee flexion and extension proprioception, and tactile sensation at the fifth metatarsal were moderately correlated with K-STARTS total and functional scores. Strength has higher levels of correlation with functional scores than proprioception.

Conclusion: Rehabilitation to promote muscle strength, proprioception and tactile sensation should be performed among patients with ACLR, muscle strength has the highest priority, followed by proprioception, with tactile sensation making the least contribution.
Deletion of classical transient receptor potential 1, 3 and 6 alters pulmonary vasoconstriction in chronic hypoxia-induced pulmonary hypertension in mice
Authors: Malkmus Kathrin , Brosien Monika , Knoepp Fenja , Schaffelhofer Lisa , Grimminger Friedrich , Rummel Christoph , Gudermann Thomas , Dietrich Alexander , Birnbaumer Lutz , Weissmann Norbert , Kraut Simone
Published: December 2022

Chronic hypoxia-induced pulmonary hypertension (CHPH) is a severe disease that is characterized by increased proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) leading to pulmonary vascular remodeling. The resulting increase in pulmonary vascular resistance (PVR) causes right ventricular hypertrophy and ultimately right heart failure. In addition, increased PVR can also be a consequence of hypoxic pulmonary vasoconstriction (HPV) under generalized hypoxia. Increased proliferation and migration of PASMCs are often associated with high intracellular Ca2+ concentration. Recent publications suggest that Ca2+-permeable nonselective classical transient receptor potential (TRPC) proteins—especially TRPC1 and 6—are crucially involved in acute and sustained hypoxic responses and the pathogenesis of CHPH. The aim of our study was to investigate whether the simultaneous deletion of TRPC proteins 1, 3 and 6 protects against CHPH-development and affects HPV in mice. We used a mouse model of chronic hypoxia as well as isolated, ventilated and perfused mouse lungs and PASMC cell cultures. Although right ventricular systolic pressure as well as echocardiographically assessed PVR and right ventricular wall thickness (RVWT) were lower in TRPC1, 3, 6-deficient mice, these changes were not related to a decreased degree of pulmonary vascular muscularization and a reduced proliferation of PASMCs. However, both acute and sustained HPV were almost absent in the TRPC1, 3, 6-deficient mice and their vasoconstrictor response upon KCl application was reduced. This was further validated by myographical experiments. Our data revealed that 1) TRPC1, 3, 6-deficient mice are partially protected against development of CHPH, 2) these changes may be caused by diminished HPV and not an altered pulmonary vascular remodeling.
Correlations between forest soil quality and aboveground vegetation characteristics in Hunan Province, China
Authors: Shen Yafei , Li Jing , Chen Fangfang , Cheng Ruimei , Xiao Wenfa , Wu Lichao , Zeng Lixiong
Published: December 2022

As a key component of terrestrial ecosystems, soil interacts directly with aboveground vegetation. Evaluating soil quality is therefore of great significance to comprehensively explore the interaction mechanism of this association. The purpose of this study was to fully understand the characteristics of aboveground vegetation, soil quality, and their potential coupling relationship among different forest types in Hunan Province, and to provide a theoretical basis for further exploring the mechanisms underlying soil–vegetation interactions in central China. We have set up sample plots of five kinds of forests (namely broad-leaved forest, coniferous forest, coniferous broad-leaved mixed forest, bamboo forest, and shrub forest) in Hunan Province. To explore the differences of vegetation characteristics and soil physical and chemical properties among the five stand types, variance analysis, principal component analysis, and regression analysis were used. Finally, we explored the coupling relationship between soil quality and aboveground vegetation characteristics of each forest. We found that there were significant differences in soil quality among the forest types, ranked as follows: shrub forest > bamboo forest > broad-leaved forest > mixed coniferous and broad-leaved forest > coniferous forest. In general, there was a negative correlation between vegetation richness and soil quality in the broad-leaved forest and the shrub forest, but they showed a positive correlation in the coniferous forest, the mixed coniferous and broad-leaved forest, and the bamboo forest. As a necessary habitat condition for aboveground vegetation, soil directly determines the survival and prosperity of plant species. These results indicated that for vegetation–soil dynamics in a strong competitive environment, as one aspect wanes the other waxes. However, in a weak competitive environment, the adverse relationship between vegetation and soil is less pronounced and their aspects can promote.
Modelling the stand dynamics after a thinning induced partial mortality: A compensatory growth perspective
Authors: Li Chao , Barclay Hugh , Huang Shongming , Roitberg Bernard , Lalonde Robert , Xu Wenli , Chen Yingbing
Published: December 2022

Introduction

With increasing forest areas under management, dynamics of managed stands have gained more attention by forest managers and practitioners. Improved understanding on how trees and forest stands would respond to different disturbances is required to predict the dynamics of managed stand.s. Partial mortality commonly occurs in stand development, and different response patterns of trees and stands to partial mortality would govern stand dynamics.

Methods

To investigate the possible response patterns using existing knowledge of growth and yield relationships, we developed TreeCG model, standing for Tree’s Compensatory Growth, a state-dependent individual tree-based forest growth model that simulates the compensatory growth of trees after experiencing a partial mortality. The mechanism behind the simulation is the redistribution of resources, including nutrients and space, freed from died trees to surviving trees. The developed new algorithm simplified the simulations of annual growth increments of individual trees over a long period of stand development.

Results

The model was able to reproduce the forest growth patterns displayed in long-term precommercial thinning experiments. The simulated forest growth displayed the process of compensatory growth from under compensation, to compensation-induced-equality, and to overcompensation over time.

Discussion

Our model can simulate stand growth trajectories after different partial harvest regimes at different times and intensities, thus support decisions in best partial harvest strategies. This generic model can be refined with given tree species and specific site conditions to predict stand dynamics after given partial mortality for any jurisdictions under management. The simulation reassembles growth trajectories of natural stands when no thinning is conducted.
Phylogenetic affiliation of endophytic actinobacteria associated with selected orchid species and their role in growth promotion and suppression of phytopathogens
Authors: Saikia Juri , Mazumdar Rajkumari , Thakur Debajit
Published: December 2022

Endophytic actinobacteria aid in plant development and disease resistance by boosting nutrient uptake or producing secondary metabolites. For the first time, we investigated the culturable endophytic actinobacteria associated with ten epiphytic orchid species of Assam, India. 51 morphologically distinct actinobacteria were recovered from surface sterilized roots and leaves of orchids and characterized based on different PGP and antifungal traits. According to the 16S rRNA gene sequence, these isolates were divided into six families and eight genera, where Streptomyces was most abundant (n=29, 56.86%), followed by Actinomadura, Nocardia, Nocardiopsis, Nocardioides, Pseudonocardia, Microbacterium, and Mycolicibacterium. Regarding PGP characteristics, 25 (49.01%) isolates demonstrated phosphate solubilization in the range of 61.1±4.4 - 289.7±11.9 µg/ml, whereas 27 (52.94%) isolates biosynthesized IAA in the range of 4.0 ± 0.08 - 43.8 ± 0.2 µg/ml, and 35 (68.62%) isolates generated ammonia in the range of 0.9 ± 0.1 - 5.9 ± 0.2 µmol/ml. These isolates also produced extracellular enzymes, viz. protease (43.13%), cellulase (23.52%), pectinase (21.56%), ACC deaminase (27.45%), and chitinase (37.25%). Out of 51 isolates, 27 (52.94%) showed antagonism against at least one test phytopathogen. In molecular screening, most isolates with antifungal and chitinase producing traits revealed the presence of 18 family chitinase genes. Two actinobacterial endophytes, Streptomyces sp. VCLA3 and Streptomyces sp. RVRA7 were ranked as the best strains based on PGP and antifungal activity on bonitur scale. GC-MS examination of ethyl acetate extract of these potent strains displayed antimicrobial compound phenol, 2,4-bis-(1,1-dimethylethyl) as the major metabolite along with other antifungal and plant growth beneficial bioactive chemicals. SEM analysis of fungal pathogen F. oxysporum (MTCC 4633) affected by Streptomyces sp. VCLA3 revealed significant destruction in the spore structure. An in vivo plant growth promotion experiment with VCLA3 and RVRA7 on chili plants exhibited statistically significant (p<0.05) improvements in all of the evaluated vegetative parameters compared to the control. Our research thus gives insight into the diversity, composition, and functional significance of endophytic actinobacteria associated with orchids. This research demonstrates that isolates with multiple plant development and broad-spectrum antifungal properties are beneficial for plant growth. They may provide a viable alternative to chemical fertilizers and pesticides and a sustainable solution for chemical inputs in agriculture.
Novel sources of drought tolerance in sorghum landraces revealed via the analyses of genotype-by-environment interactions
Authors: Enyew Muluken , Carlsson Anders S. , Geleta Mulatu , Tesfaye Kassahun , Hammenhag Cecilia , Seyoum Amare , Feyissa Tileye
Published: December 2022

Globally, sorghum is the fifth most important crop, which is used for food, feed and fuel. However, its production and productivity are severely limited by various stresses, including drought. Hence, this study aimed to determine the responses of different drought-tolerance related traits in the Ethiopian sorghum germplasm through multi-environment field trials, thereby identifying novel sources of germplasm that can be used for breeding the crop for drought-tolerance. Three hundred twenty sorghum landraces and four improved varieties were grown at three sites within drought-prone areas (Melkassa, Mieso and Mehoni) in Ethiopia. The targeted traits were chlorophyll content at flowering (CHLF), chlorophyll content at maturity (CHLM), green leaf number at flowering (GLNF), stay-green (SG), flag leaf area (FLA), peduncle length (PDL), and panicle exertion (PAE). Multi-variate analyses of the collected data revealed the presence of high phenotypic variation in all traits. The combined and AMMI Analysis of variance showed that phenotypic variation due to the genotypes was higher for SG, CHLM, CHLF and GLNF and lower for FLA, PE and PDL in comparison with variation due to the environments or genotype by environment interactions. High broad sense heritability was observed for CHLF, CHLM, SG, GLNF, FLA, and PDL, whereas PAE showed moderate heritability. Due to the high heritability of chlorophyll content and the relatively small effect of environmental factors on it, it could serve as a criterion for selecting desirable genotypes for drought-tolerant breeding in sorghum. It has been found that chlorophyll content has a significant positive correlation with stay-green and grain yield, indicating that high chlorophyll content contributes to increasing grain yield by delaying the process of leaf senescence. The analyses of AMMI, GGE biplot, and genotype selection index revealed that several sorghum landraces outperformed the improved varieties with respect to CHLF, CHLM, and SG. Such landraces could serve as novel sources of germplasm for improving drought tolerance through breeding.
The moderating effect of marital conflict on the relationship between social avoidance and socio-emotional functioning among young children in suburban China
Authors: Zhu Jingjing , Liu Mowei , Shu Xin , Xiang Shuhui , Jiang Yaoqin , Li Yan
Published: December 2022

Social avoidance has been found to be related to negative social adjustment, yet evidence of the relationship between social avoidance and social adjustment is very limited in suburban preschoolers in China. Moreover, the underlying mechanisms that help to explain the associations between social avoidance and socio-emotional adjustment remain poorly considered. The goal of the present study was to examine the moderating effect of marital conflict in the link between social avoidance and indices of socio-emotional functioning among young children in mainland China. Participants were N = 256 children aged from 49 to 72 months (125 boys, 131 girls, Mage = 59.06 months, SD = 6.58) attending preschools/kindergartens in suburban areas of Shanghai, People’s Republic of China. Multi-source assessments were employed, with mothers reporting marital conflict as well as rating their children’s social withdrawal (i.e., social avoidance, shyness, unsociability), and teachers assessing indices of children’s socio-emotional functioning at school (i.e., anxious-fearful behavior, peer exclusion, and loneliness). Children were asked about their loneliness. Among the results, social avoidance was positively associated with anxious-fearful behavior, peer exclusion, and loneliness (marginal significance). Moreover, as hypothesized, marital conflict was found to exacerbate the relations between social avoidance and peer exclusion, and loneliness. Results are discussed in terms of the etiology and implications of social avoidance among young children in mainland China.
The importance of the comparative benchmark for measuring composite financial literacy with survey data
Authors: Liu Fang , Lei Hanyun , Lu Wenyi , Yin Qiang
Published: December 2022

Introduction

Using survey data to calculate composite financial literacy (CFL), existed studies do not consider the geographical difference of the means of objectively-measured financial literacy and subjectively-perceived financial literacy, i.e., comparative benchmark.

Methods

Taking the survey data of National Financial Capability Study (NFCS) for example, we explain why it is more reasonable to use the within-state average rather than the national average of financial literacy as the comparative benchmark to measure CFL. Then we use NFCS 2009, 2012, 2015 and 2018 dataset to comparatively analyze the difference between CFL measured with the two benchmarks.

Results

The results of statistical analysis show that there is a great difference among the four groups of CFL measured with the two benchmarks, and 10.7% of respondents are categorized as a particular group of CFL incorrectly for all datasets. Additionally, the findings of spatial distribution analysis unveils that 36, 19, 15, and 6 states have respondents miscategorized in the four groups of CFL for 2009, 2012, 2015, and 2018 respectively, in which the highest proportion of the population miscategorized in a state is up to 49.91%. Finally, we find that several groups of CFL measured with the two benchmarks have significantly different effects on stock market participation behavior.

Discussion

Using the national average as a benchmark to determine all the respondents’ relative financial literacy levels for different states is not meaningful, and will lose the practical appeal to tackle the regional inequalities of financial literacy among the households. Therefore, we suggest that the within-state average of financial literacy, not the national average, should be taken as the comparative benchmark for identifying the more precise groups of CFL in survey.
How does rapport impact knowledge transfer from older to younger employees? The moderating role of supportive climate
Authors: Rui Hainan , Ju Hailong
Published: December 2022

Introduction

Knowledge transfer from older to younger employees plays a key role in lessening knowledge loss and maintaining firms' competitiveness. While the disharmony derived from a salient age difference between younger and older workers hinders such knowledge transfer. This study aims to construct a rapport model to address it.

Methods

Data from 318 respondents in various industries were collected through a questionnaire-based survey to test the proposed model. The research hypotheses were tested using hierarchical multiple regressions.

Results

Our empirical results show that almost all rapport dimensions facilitate such knowledge transfer; The moderating role of supportive climate is strong that it enhances or replaces the effects of rapport dimensions on such knowledge transfer.

Discussion

This study contributes to research on knowledge transfer and rapport by providing a detailed understanding of the relational mechanism of the knowledge transfer from older to younger employees based on a revised model of rapport. It also serves as a reference for firms to leverage rapport-building and a supportive climate to enhance this invaluable knowledge transfer.
To explore the impact of augmented reality digital picture books in environmental education courses on environmental attitudes and environmental behaviors of children from different cultures
Authors: Chen Shih-Yeh
Published: December 2022

Introduction

Environmental education has long been closely related to sustainable development. In this study, in response to the United Nations Sustainable Development Goals (SDGs), an augmented reality (AR) digital picture book was created using the unique natural ecosystem of Taiwan’s Orchid Island as a source of self-efficacy for science learning.

Methods

Interactive environmental education learning through AR drawing was used to determine whether students’ science learning self-efficacy and environmental attitudes significantly influenced the environmental behavioral skills of culturally diverse children. In this study, 26 elementary sixth-grade Taiwanese students and 26 elementary sixth-grade Japanese students were invited to participate in an extended reality drawing activity as an environmental education curriculum.

Results

Based on the sample size of 52, the survey results were accurate with a sampling error of 3.8% with a confidence level of 95%. A questionnaire survey was administered to the 52 students after the event. After the valid questionnaire samples were collected, a partial least squares structural equation modeling (PLS-SEM) analysis was conducted with Smart PLS 3.0 on the small sample. The results of the study showed that students who had a better self-efficacy in science learning were more likely to engage in conservation actions related to the natural environment in their daily lives.

Discussion

In this study, the constructs of environmental behavior were further discussed and the hypothesis model was validated using the quantitative empirical method. The results of the study revealed good reliability, convergent validity, and discriminant validity of the constructs in the hypothesis model, and the hypothesis model itself was validated. In the validated model, students’ science learning self-efficacy affects the sustainability of their environmental behaviors, but only through the role of environmental attitudes. However, the environmental attitudes construct plays a fully mediating role in the model.
Vlogger's persuasive strategy and consumers' purchase intention: The dual mediating role of para-social interactions and perceived value
Authors: Sheng Xiayan , Zeng Zhenhua , Zhang Wen , Hu Yuanhui
Published: December 2022

It is a new advertising marketing method for commodity companies to use vloggers to endorse their products, and to influence consumers' attitudes and decisions. In view of this phenomenon, there are few studies on the relationship between vloggers and consumers, and this study aims to explore how the persuasive strategies used by vloggers to promote products influence consumers' purchase intentions. Based on the concepts of Aristotle's persuasion theory, this study extracts two specific persuasive approaches, “two-sided messages” and “emotional appeal,” to explore consumers' perceptions of them and the effectiveness of these two strategies. At the same time, para-social interaction and perceived value as intermediary factors are also included in the study for further discussion. The study empirically analyzed a sample of 511 questionnaires from participants who had purchased products recommended by vloggers and came to the following conclusions: (1) vloggers can enhance consumers' purchase intention by adopting two-sided messages persuasion when promoting products; (2) vloggers' emotional persuasion can enhance consumers' purchase intention; (3) as an intermediary variable, para-social interaction plays a more obvious role in vloggers' persuasion by appealing to emotions. The audience can have common feelings with vloggers, and they are more connected with each other, thus increasing their willingness to buy; (4) Perceived value, as an intermediary variable, plays a more obvious role in vloggers' persuasion with the two-sided messages. The two-sided messages can show vloggers' credibility and more abundant information about products, so that consumers have a positive perception of product value, and the direct persuasion effect of the two-sided messages is greater. Based on the results of the study, this paper helps vloggers to adopt different persuasive approaches for different types of audiences, choose the proper marketing methods, attract the potential customers, and achieve such purposes as enriching product marketing forms and increasing market share.
Appropriating Shakespearean graphic novels for Malaysian classrooms to create correct gender representations
Authors: Ismail Hanita Hanim , Azizan Mazlin , Rashid Radzuwan Ab , Asif Muhammad
Published: December 2022

Public awareness on gender education may not be easily translated in living classrooms, which prompts new alternatives. In this study, we explored Shakespearean graphic novels in exposing Malaysian school students to gender-related issues. The two-fold research entails tracing the female presence in eight selected images from digital graphic novels that define the gender and place social expectations across the globe; and secondly, gathering teachers’ perception of these selected graphic novels. The chosen images were divided into two categories—those published as the cover pages of graphic novels and those that were contested for the Graphic Shakespeare Competition established in 2016. These stereotypical images of women are inconsistent with the objective of achieving inclusivity and correct gender representations. The data analysed based on a semi-structured interview on six ESL teachers suggest that as much as the graphic novels are seen as valuable in pedagogical contexts and in exposing the students to learning English and gender representations through literature, the material selection, and pedagogical approaches, including determining the classroom activities must be cautiously considered in terms of their cultural appropriateness to ensure students’ readiness and effective outcomes. Discussing these pertinent issues, especially in addressing gender (mis)representations relevant to education, helps pave a new route within the UN SDG Goal 5 where gender nuances and phrases ought to be carefully constructed in a new narrative that shapes global perception towards women.
How does leaders' information-sharing behavior affect subordinates' taking charge behavior in public sector? A moderated mediation effect
Authors: Liu Jun-Na , Hou Yun-Zhang , Wang Jun , Fu Ping , Xia Cong-Zhen
Published: December 2022

Introduction

Taking charge behavior (TCB) of civil servants is an important part of individual innovation performance, which is not only a key step for innovation in the public but also a real need for high-quality cadres construction in the public sector in the new era. Therefore, it is necessary to carry out an in-depth discussion on civil servants' taking charge behavior. Based on the theory of planned behavior, this paper constructs the framework of"cognition-motivation-behavior" to deeply explore the relationship between public sector leaders' information-sharing behavior and subordinates' taking charge behavior, as well as the mediating and moderating effects of subordinates' public service motivation and emotional trust.

Method

This study collected 200 civil servants' questionnaires by online survey, and conducted regression analysis through SPSS/AMOS/PROCESS.

Result and discussion

The empirical study finds that the information-sharing behavior of leaders in the public sector can significantly affect the TCB of subordinates; the public service motivation partially mediates the relationship between them; emotional trust positively moderates the mediation effect of public service motivation in the relationship between leaders' information-sharing behavior and subordinates' TCB in the public. This study not only enriches the research on civil servants' TCB theoretically but also provides meaningful enlightenment for promoting civil servants' taking charge behavior.