bluuebunny/crossref_metadata_2025 huggingface dataset:
A technology platform for standardized cryoprotection and freezing of large-volume brain tissues for high-resolution histology
Authors: Kumarasami Ramdayalan , Verma Richa , Pandurangan Karthika , Ramesh Jivitha Jyothi , Pandidurai Sathish , Savoia Stephen , Jayakumar Jaikishan , Bota Mihail , Mitra Partha , Joseph Jayaraj , Sivaprakasam Mohanasankar
Published: November 2023

Understanding and mapping the human connectome is a long-standing endeavor of neuroscience, yet the significant challenges associated with the large size of the human brain during cryosectioning remain unsolved. While smaller brains, such as rodents and marmosets, have been the focus of previous connectomics projects, the processing of the larger human brain requires significant technological advancements. This study addresses the problem of freezing large brains in aligned neuroanatomical coordinates with minimal tissue damage, facilitating large-scale distortion-free cryosectioning. We report the most effective and stable freezing technique utilizing an appropriate choice of cryoprotection and leveraging engineering tools such as brain master patterns, custom-designed molds, and a continuous temperature monitoring system. This standardized approach to freezing enables high-quality, distortion-free histology, allowing researchers worldwide to explore the complexities of the human brain at a cellular level. Our approach combines neuroscience and engineering technologies to address this long-standing challenge with limited resources, enhancing accessibility of large-scale scientific endeavors beyond developed countries, promoting diverse approaches, and fostering collaborations.
Short-term postsynaptic plasticity facilitates predictive tracking in continuous attractors
Authors: Zhao Huilin , Yang Sungchil , Fung Chi Chung Alan
Published: November 2023

Introduction

The N-methyl-D-aspartate receptor (NMDAR) plays a critical role in synaptic transmission and is associated with various neurological and psychiatric disorders. Recently, a novel form of postsynaptic plasticity known as NMDAR-based short-term postsynaptic plasticity (STPP) has been identified. It has been suggested that long-lasting glutamate binding to NMDAR allows for the retention of input information in brain slices up to 500 ms, leading to response facilitation. However, the impact of STPP on the dynamics of neuronal populations remains unexplored.

Methods

In this study, we incorporated STPP into a continuous attractor neural network (CANN) model to investigate its effects on neural information encoding in populations of neurons. Unlike short-term facilitation, a form of presynaptic plasticity, the temporally enhanced synaptic efficacy resulting from STPP destabilizes the network state of the CANN by increasing its mobility.

Results

Our findings demonstrate that the inclusion of STPP in the CANN model enables the network state to predictively respond to a moving stimulus. This nontrivial dynamical effect facilitates the tracking of the anticipated stimulus, as the enhanced synaptic efficacy induced by STPP enhances the system's mobility.

Discussion

The discovered STPP-based mechanism for sensory prediction provides valuable insights into the potential development of brain-inspired computational algorithms for prediction. By elucidating the role of STPP in neural population dynamics, this study expands our understanding of the functional implications of NMDAR-related plasticity in information processing within the brain.

Conclusion

The incorporation of STPP into a CANN model highlights its influence on the mobility and predictive capabilities of neural networks. These findings contribute to our knowledge of STPP-based mechanisms and their potential applications in developing computational algorithms for sensory prediction.
Recovery from shift work
Authors: Kalkanis Alexandros , Demolder Saartje , Papadopoulos Dimitrios , Testelmans Dries , Buyse Bertien
Published: November 2023

One fifth of today's workforce is engaged in shift work and exposed to various mental and physical health risks including shift work disorder. Efficiently recovering from shift work through physical and mental interventions allows us to mitigate negative effects on health, enables a better work-life balance and enhances our overall wellbeing. The aim of this review is to provide a state-of-the-art overview of the available literature. The role of sleep timing and naps, light therapy and psychotherapy, diet and exercise in recovery from shift work is presented here. We further review the impact of shift schedules and social support on post-shift unwinding.
The effects of trauma on feedback processing: an MEG study
Authors: Sawalma Abdulrahman S. , Kiefer Christian M. , Boers Frank , Shah N. Jon , Khudeish Nibal , Neuner Irene , Herzallah Mohammad M. , Dammers Jürgen
Published: November 2023

The cognitive impact of psychological trauma can manifest as a range of post-traumatic stress symptoms that are often attributed to impairments in learning from positive and negative outcomes, aka reinforcement learning. Research on the impact of trauma on reinforcement learning has mainly been inconclusive. This study aimed to circumscribe the impact of psychological trauma on reinforcement learning in the context of neural response in time and frequency domains. Two groups of participants were tested - those who had experienced psychological trauma and a control group who had not - while they performed a probabilistic classification task that dissociates learning from positive and negative feedback during a magnetoencephalography (MEG) examination. While the exposure to trauma did not exhibit any effects on learning accuracy or response time for positive or negative feedback, MEG cortical activity was modulated in response to positive feedback. In particular, the medial and lateral orbitofrontal cortices (mOFC and lOFC) exhibited increased activity, while the insular and supramarginal cortices showed decreased activity during positive feedback presentation. Furthermore, when receiving negative feedback, the trauma group displayed higher activity in the medial portion of the superior frontal cortex. The timing of these activity changes occurred between 160 and 600 ms post feedback presentation. Analysis of the time-frequency domain revealed heightened activity in theta and alpha frequency bands (4–10 Hz) in the lOFC in the trauma group. Moreover, dividing the two groups according to their learning performance, the activity for the non-learner subgroup was found to be lower in lOFC and higher in the supramarginal cortex. These differences were found in the trauma group only. The results highlight the localization and neural dynamics of feedback processing that could be affected by exposure to psychological trauma. This approach and associated findings provide a novel framework for understanding the cognitive correlates of psychological trauma in relation to neural dynamics in the space, time, and frequency domains. Subsequent work will focus on the stratification of cognitive and neural correlates as a function of various symptoms of psychological trauma. Clinically, the study findings and approach open the possibility for neuromodulation interventions that synchronize cognitive and psychological constructs for individualized treatment.
Perioperative cognition in association with malnutrition and frailty: a narrative review
Authors: Dammavalam Vikalpa , Murphy Jasper , Johnkutty Meenu , Elias Murad , Corn Ryan , Bergese Sergio
Published: November 2023

Postoperative delirium (POD) is a prevalent clinical entity characterized by reversible fluctuating altered mental status and cognitive impairment with acute and rapid onset a few days after major surgery. Postoperative cognitive decline (POCD) is a more permanent extension of POD characterized by prolonged global cognitive impairment for several months to years after surgery and anesthesia. Both syndromes have been shown to increase morbidity and mortality in postoperative patients making their multiple risk factors targets for optimization. In particular, nutrition imparts a significant and potentially reversible risk factor. Malnutrition and frailty have been linked as risk factors and predictive indicators for POD and less so for POCD. This review aims to outline the association between nutrition and perioperative cognitive outcomes as well as potential interventions such as prehabilitation.
Improvements during long-term fasting in patients with long COVID – a case series and literature review
Authors: Grundler Franziska , Mesnage Robin , Cerrada Alberto , Wilhelmi de Toledo Françoise
Published: November 2023

Background

Post-acute sequelae of a severe acute respiratory syndrome coronavirus 2 infection, also known as long COVID, comprises a variety of symptoms that impair the quality of life. This represents a growing public health burden, with millions of individuals worldwide affected.

Case description

We present a case series of 14 COVID-19 patients with post-acute symptoms who underwent medically supervised long-term fasting (6 to 16 days) according to the peer-reviewed Buchinger Wilhelmi protocol. The EQ-5D-5L questionnaire and visual scales were used to evaluate the intensity of the symptoms, retrospectively during the acute phase, and prospectively before and after long-term fasting. Blood tests were also performed before and after fasting. Thirteen patients reported that fasting caused an enhancement in their perceived overall health. Only one patient had no improvement. Both frequent (fatigue, breathlessness, muscle and joint pains) and less frequent (cognitive impairment, smell and taste disorders) sequelae ameliorated. Body weight and other risk factors for cardiometabolic diseases like blood pressure, blood glucose, total cholesterol, low-density-lipoprotein cholesterol, and triglycerides were reduced. No severe side effects occurred.

Discussion

This case series reports beneficial changes in self-perceived symptoms in patients with long COVID after long-term fasting. This highlights the potential of long-term fasting as an effective intervention for managing and treating long COVID.
Genome-wide association study of powdery mildew resistance in cultivated soybean from Northeast China
Authors: Sang Yongsheng , Zhao Hongkun , Liu Xiaodong , Yuan Cuiping , Qi Guangxun , Li Yuqiu , Dong Lingchao , Wang Yingnan , Wang Dechun , Wang Yumin , Dong Yingshan
Published: November 2023

Powdery mildew (PMD), caused by the pathogen Microsphaera diffusa, leads to substantial yield decreases in susceptible soybean under favorable environmental conditions. Effective prevention of soybean PMD damage can be achieved by identifying resistance genes and developing resistant cultivars. In this study, we genotyped 331 soybean germplasm accessions, primarily from Northeast China, using the SoySNP50K BeadChip, and evaluated their resistance to PMD in a greenhouse setting. To identify marker-trait associations while effectively controlling for population structure, we conducted genome-wide association studies utilizing factored spectrally transformed linear mixed models, mixed linear models, efficient mixed-model association eXpedited, and compressed mixed linear models. The results revealed seven single nucleotide polymorphism (SNP) loci strongly associated with PMD resistance in soybean. Among these, one SNP was localized on chromosome (Chr) 14, and six SNPs with low linkage disequilibrium were localized near or in the region of previously mapped genes on Chr 16. In the reference genome of Williams82, we discovered 96 genes within the candidate region, including 17 resistance (R)-like genes, which were identified as potential candidate genes for PMD resistance. In addition, we performed quantitative real-time reverse transcription polymerase chain reaction analysis to evaluate the gene expression levels in highly resistant and susceptible genotypes, focusing on leaf tissues collected at different times after M. diffusa inoculation. Among the examined genes, three R-like genes, including Glyma.16G210800, Glyma.16G212300, and Glyma.16G213900, were identified as strong candidates associated with PMD resistance. This discovery can significantly enhance our understanding of soybean resistance to PMD. Furthermore, the significant SNPs strongly associated with resistance can serve as valuable markers for genetic improvement in breeding M. diffusa-resistant soybean cultivars.
Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings
Authors: Slocum Robert D. , Mejia Peña Carolina , Liu Zhongchi
Published: November 2023

In Arabidopsis seedlings, inhibition of aspartate transcarbamoylase (ATC) and de novo pyrimidine synthesis resulted in pyrimidine starvation and developmental arrest a few days after germination. Synthesis of pyrimidine nucleotides by salvaging of exogenous uridine (Urd) restored normal seedling growth and development. We used this experimental system and transcriptional profiling to investigate genome-wide responses to changes in pyrimidine availability. Gene expression changes at different times after Urd supplementation of pyrimidine-starved seedlings were mapped to major pathways of nucleotide metabolism, in order to better understand potential coordination of pathway activities, at the level of transcription. Repression of de novo synthesis genes and induction of intracellular and extracellular salvaging genes were early and sustained responses to pyrimidine limitation. Since de novo synthesis is energetically more costly than salvaging, this may reflect a reduced energy status of the seedlings, as has been shown in recent studies for seedlings growing under pyrimidine limitation. The unexpected induction of pyrimidine catabolism genes under pyrimidine starvation may result from induction of nucleoside hydrolase NSH1 and repression of genes in the plastid salvaging pathway, diverting uracil (Ura) to catabolism. Identification of pyrimidine-responsive transcription factors with enriched binding sites in highly coexpressed genes of nucleotide metabolism and modeling of potential transcription regulatory networks provided new insights into possible transcriptional control of key enzymes and transporters that regulate nucleotide homeostasis in plants.
Communication with cancer patients: the perspective of caregivers versus non-caregivers in Iran
Authors: Naghavi Azam , Salimi Samaneh , Rief Winfried , von Blanckenburg Pia
Published: November 2023

Objective

This study investigated and compared the attitudes of healthy Iranian individuals (n = 302) in forms of two groups of caregivers and non-caregivers of cancer patients about the communication with cancer patients, and their personal wish to know the diagnosis if they ever had cancer. In addition, this study aimed to identify how many participants in the caregivers’ group had spoken with their family member affected by cancer about their illness.

Methods

Caregivers (50.7%) and non-caregivers (49.3%) responded to two questionnaires regarding their general attitudes about communicating with cancer patients, and their willingness to know about their illness if they had cancer.

Results

The majority of participants (92.8%), especially in the caregiver group, agreed with the right of patients to know the diagnosis and prognosis, and also wished to know if they ever had cancer. However, around 64% of caregivers never talked about cancer with the affected patients.

Conclusion

Participants generally believed that patients have the right to know the diagnosis and prognosis, and they also wished to know if they ever had cancer. However, in reality many cancer patients are not included in communication sessions in Iran. Health professionals should focus on how to create a balance between medical bioethics with cultural influences on communication with patients.
Artificial intelligence-based language learning: illuminating the impact on speaking skills and self-regulation in Chinese EFL context
Authors: Qiao Hongliang , Zhao Aruna
Published: November 2023

Introduction

This study investigated the effectiveness of artificial intelligence-based instruction in improving second language (L2) speaking skills and speaking self-regulation in a natural setting. The research was conducted with 93 Chinese English as a foreign language (EFL) students, randomly assigned to either an experimental group receiving AI-based instruction or a control group receiving traditional instruction.

Methods

The AI-based instruction leveraged the Duolingo application, incorporating natural language processing technology, interactive exercises, personalized feedback, and speech recognition technology. Pre- and post-tests were conducted to assess L2 speaking skills and self-regulation abilities.

Results

The results of the study demonstrated that the experimental group, which received AI-based instruction, exhibited significantly greater improvement in L2 speaking skills compared to the control group. Moreover, participants in the experimental group reported higher levels of self-regulation.

Discussion

These findings suggest that AI-based instruction effectively enhances L2 speaking skills and fosters self-regulatory processes among language learners, highlighting the potential of AI technology to optimize language learning experiences and promote learners’ autonomy and metacognitive strategies in the speaking domain. However, further research is needed to explore the long-term effects and specific mechanisms underlying these observed improvements.
PCR standard curve quantification in an extensive wastewater surveillance program: results from the Dutch SARS-CoV-2 wastewater surveillance
Authors: Nagelkerke Erwin , Hetebrij Wouter A. , Koelewijn Jaap M. , Kooij Jannetje , van der Drift Anne-Merel R. , van der Beek Rudolf F. H. J. , de Jonge Eline F. , Lodder Willemijn J.
Published: November 2023

Since the start of the COVID-19 pandemic in 2020, wastewater surveillance programs were established, or upscaled, in many countries around the world and have proven to be a cost-effective way of monitoring infectious disease pathogens. Many of these programs use RT-qPCR, and quantify the viral concentrations in samples based on standard curves, by including preparations of a reference material with known nucleic acid or virus concentrations in the RT-qPCR analyses. In high-throughput monitoring programs it is possible to combine data from multiple previous runs, circumventing the need for duplication and resulting in decreased costs and prolonged periods during which the reference material is obtained from the same batch. However, over time, systematic shifts in standard curves are likely to occur. This would affect the reliability and usefulness of wastewater surveillance as a whole. We aim to find an optimal combination of standard curve data to compensate for run-to-run measurement variance while ensuring enough flexibility to capture systematic longitudinal shifts. Based on more than 4000 observations obtained with the CDC N1 and N2 assays, taken as a part of the National Sewage Surveillance program at the Dutch National Institute for Public Health and the Environment, we show that seasonal and long-term shifts in RT-qPCR efficiency and sensitivity occur. We find that in our setting, using five days of standard-curve data to quantify, results in the least error prone curve or best approximation. This results in differences up to 100% in quantified viral loads when averaged out over a nationwide program of >300 treatment plants. Results show that combining standard curves from a limited set of runs can be a valid approach to quantification without obscuring the trends in the viral load of interest.
Children can rate perceived effort but do not follow intensity instructions during soccer training
Authors: Reinke Marco , Schmitz Gerd
Published: November 2023

The perception of effort is elementary for the self-regulation of exercise intensity in sports. The competence for rating perceived effort (RPE) seems to be related to physical and cognitive development. Children accurately rate perceived effort during incremental exercise tests when loads progressively increase, but it remains unclear how children perform when they participate in sports games, which are characterized by complex tasks with varying intensity profiles. The present study investigates children's competencies for rating perceived effort and producing predetermined intensities during soccer training. Twenty-five children aged 11–13 years performed two similar training sessions. In the first session, the children trained without intensity instructions and continuously rated their effort. In the second session, the children were instructed to produce predefined intensities. Before the first training session, executive functions were assessed by cognitive performance tests and a self-report measure. RPE correlated significantly with heart rate measures (R2 = 0.27, p < 0.001). As confirmed by factor analysis, individual differences in these correlations were related to the outcomes of the cognitive tests and the self-report measure. RPE in training session 2 differed from RPE in training session 1 (d = 1.22, p < 0.001), although the heart rate data did not differ significantly between training sessions (d = −0.19, p = 0.780). Thirteen-year-old children performed significantly better than eleven-year-old children (d = 1.69, p = 0.027). The results suggest that children are able to rate perceived effort during soccer training and that this ability is related to executive functions. Conversely, children may not be able to alter their intensities in response to instructions, although their ratings suggest that they have largely succeeded in doing so.
Elite athletes: what lessons for the future can be learned from their responses to the social disruption caused by the COVID-19 pandemic?
Authors: Poteko Kaja , Coakley Jay , Doupona Mojca
Published: November 2023

In mid-March 2020, the Covid-19 pandemic was declared, disrupting established routines and impacting every aspect of our lives. Sport as a social phenomenon was no exception. On the one hand, with the suspension and postponement of competitions and the various restrictions, the pandemic seemed to stop time, destroying the previously familiar ways of functioning of the sports sector, and forcing it to constantly reinvent, restructure and adapt. On the other hand, the changed situation highlighted the problems and inequalities that sport had faced long before and that the pandemic had mostly exacerbated, but not caused. In this review article, we identify the lessons and insights the pandemic has brought, especially for elite athletes. Because many scientific articles have emerged in connection with the pandemic, we select and review them according to our focus on elite athletes and summarise their findings. We then use those findings to derive guidelines that can serve top athletes as a tool in potentially similar situations in the future. Our proposed guidelines are divided into macro-, meso-, and micro-social levels, encompassing national and international, organizational and individual perspectives.
Identification and characterization of porcine Rotavirus A in Chilean swine population
Authors: Neira Victor , Melgarejo Cristián , Urzúa-Encina Constanza , Berrios Felipe , Valdes Valentina , Mor Sunil , Brito-Rodriguez Barbara , Ramirez-Toloza Galia Andrea
Published: November 2023

Rotavirus A (RVA) is a common cause of diarrhea in newborn pigs, leading to significant economic losses. RVA is considered a major public health concern due to genetic evolution, high prevalence, and pathogenicity in humans and animals. The objective of this study was to identify and characterize RVA in swine farms in Chile. A total of 154 samples (86 oral fluids and 68 fecal samples) were collected, from 22 swine farms. 58 (38%) samples belonging to 14 farms were found positive for RVA by real-time RT-PCR. The samples with low Ct values (21) and the two isolates were selected for whole genome sequencing. Nearly complete genomes were assembled from both isolates and partial genomes were assembled from five clinical samples. BLAST analysis confirmed that these sequences are related to human and swine-origin RVA. The genomic constellation was G5/G3-P[7]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Phylogenetic analysis showed that VP4, VP1, VP2, NSP2, NSP3, NSP4, and NSP5 sequences were grouped in monophyletic clusters, suggesting a single introduction. The phylogenies for VP7, VP6, VP3, and NSP1 indicated two different origins of the Chilean sequences. The phylogenetic trees showed that most of the Chilean RVA sequences are closely related to human and swine-origin RVA detected across the world. The results highlight the potential zoonotic nature of RVA circulating in Chilean swine farms. Therefore, it is important to continue RVA whole genome sequencing globally to fully understand its complex epidemiology and early detection and characterization of zoonotic strains.
“Pseudo-Contracted” Workers as a Means of Bypassing Labour Law in Greece
Authors: Rompoti Eleni D. , Ioannides Alexis D.
Published: November 2023

The aim of this article is to investigate two external flexible forms of employment—the leasing of workers through Temporary Work Agencies (TWAs) and the contracted workers employed through Business Service Providers (contractors). Undoubtedly, these two forms of employment are complex and often give rise to confusion. First, this article reviews the characteristics of these types of workers and the operation of these businesses. Second, it presents the results of a mixed method of empirical research (quantitative and qualitative) regarding contracted workers. Our sample was 365 contracted workers from the cities of Athens, Thessaloniki and Patras, Greece. In particular, quantitative research is conducted using a methodology called RDS (Respondent Driven Sampling) that is innovative in the field of labour economics and labour relations. Some significant findings of our qualitative research are used to improve, extend, and interpret the quantitative results. Our research proves that contracted workers, who are employed at the premises of the banks, are leased workers, and the contracting undertakings usually operate unlawfully as TWAs. Our research proves that Banks in Greece are using “pseudo-contracting” to circumvent the European Directive 2008/104/EC and the Greek Laws 4052/2012 and 4254/2014, both of which provide institutional protection to workers leased through TWAs. In more detail, the relevant European Directive and the Greek Law 4052/2012 provide salary equality and equal labour rights for the leased workers in Greece and the EU, when they share the same qualifications as the permanent employees of the user undertakings. The employers’ aim in adopting this policy is mainly to pay lower salaries to contracted workers, who in practice have the characteristics of leased workers.
Role of Free-Ranging Synanthropic Egyptian Geese (Alopochen aegyptiaca) as Natural Host Reservoirs for Salmonella spp. in Germany
Authors: Fischer Ella F. , Müller Romy , Todte Matthias , Taubert Anja , Hermosilla Carlos
Published: November 2023

Salmonella is one of the most common and pathogenic bacteria worldwide, causing severe enteritis in humans and representing a relevant intestinal illness in One Health for young, old and immunosuppressed patients. Various Salmonella serovars have been described to be responsible for human Salmonellosis. Birds represent natural carriers of different zoonotic-relevant Salmonella serovars and Anseriformes can not only transmit Salmonella spp. to humans but also manifest clinical Salmonellosis. In this study, 138 scat samples (n = 138) of free-ranging Egyptian geese (EG; Alopochen aegyptiaca) were collected in Germany, including 83 scat samples from city parks, 30 samples from 14 public swimming pools and 25 fresh caecal samples of dead EG. Collected EG scat samples were examined for the presence of Salmonella spp. according either to the ISO 6579 (2017) norm or to a combination of bacterial pre-enrichment and specific PCR for detection of Salmonella DNA. All 138 analysed EG faecal samples resulted Salmonella-negative. Furthermore, the survival of Salmonella enterica subsp. enterica Serovar Anatum in spiked EG droppings was tested in four different concentrations of chlorinated pool water. In vitro testing demonstrated that S. Anatum-spiked EG droppings were still infectious for up to six hours in chlorinated pool water according to current German regulations for public swimming pools. This study is to be considered as a baseline investigation to clarify the role of synanthropic EG as natural carriers of zoonotic Salmonella in cities; nonetheless, large-scale epidemiological studies, including higher numbers of samples as well as more urban locations, are needed for final conclusions on the occurrence of this intestinal bacteria in neozootic EG.
Are Veterinary Costs and Socioeconomic Status Risk Factors for Companion Animal Relinquishment in the Republic of Korea?
Authors: Rah HyungChul , Choi Seok-Hwa
Published: November 2023

More than 110,000 companion animals are sent to shelters each year due to abandonment in Republic of Korea, and there is a need to analyze the causes of the relinquishment of animals and implement appropriate policies. Veterinary costs have been blamed for this issue in Republic of Korea above the reported leading causes of socioeconomic status of owners, cost and behavior issues of the animals, or housing restrictions. However, it is rare to find supporting evidence. In this study, we aimed to determine whether veterinary costs and socioeconomic factors are related to animal relinquishment in Republic of Korea. Multiple regression models were used to test if veterinary costs and socioeconomic indicators can account for relinquishment in 128 regions of Republic of Korea in 2020 and 2021. When five independent variables (two veterinary cost data and three socioeconomic indicators) were included, the regression model showed significance in explaining pet relinquishment in 2020, with an adjusted coefficient of determination of 0.3956. Pet relinquishment can also be explained by the same five variables for 2021, with an adjusted coefficient of determination of 0.391 with p < 0.0001. The findings suggest that intervention to reduce companion animal relinquishment in Republic of Korea should focus on lightening the financial burdens of owners as the socioeconomic status of a community worsens.
Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation
Authors: Giuliani Angelica , Giudetti Anna Maria , Vergara Daniele , Del Coco Laura , Ramini Deborah , Caccese Sara , Sbriscia Matilde , Graciotti Laura , Fulgenzi Gianluca , Tiano Luca , Fanizzi Francesco Paolo , Olivieri Fabiola , Rippo Maria Rita , Sabbatinelli Jacopo
Published: November 2023

Cellular senescence is closely linked to endothelial dysfunction, a key factor in age-related vascular diseases. Senescent endothelial cells exhibit a proinflammatory phenotype known as SASP, leading to chronic inflammation (inflammaging) and vascular impairments. Albeit in a state of permanent growth arrest, senescent cells paradoxically display a high metabolic activity. The relationship between metabolism and inflammation is complex and varies across cell types and senescence inductions. While some cell types shift towards glycolysis during senescence, others favor oxidative phosphorylation (OXPHOS). Despite the high availability of oxygen, quiescent endothelial cells (ECs) tend to rely on glycolysis for their bioenergetic needs. However, there are limited data on the metabolic behavior of senescent ECs. Here, we characterized the metabolic profiles of young and senescent human umbilical vein endothelial cells (HUVECs) to establish a possible link between the metabolic status and the proinflammatory phenotype of senescent ECs. Senescent ECs internalize a smaller amount of glucose, have a lower glycolytic rate, and produce/release less lactate than younger cells. On the other hand, an increased fatty acid oxidation activity was observed in senescent HUVECs, together with a greater intracellular content of ATP. Interestingly, blockade of glycolysis with 2-deoxy-D-glucose in young cells resulted in enhanced production of proinflammatory cytokines, while the inhibition of carnitine palmitoyltransferase 1 (CPT1), a key rate-limiting enzyme of fatty acid oxidation, ameliorated the SASP in senescent ECs. In summary, metabolic changes in senescent ECs are complex, and this research seeks to uncover potential strategies for modulating these metabolic pathways to influence the SASP.
Goji Berry in the Diet of the Rabbit Buck: Effects on Semen Quality, Oxidative Status and Histological Features of the Reproductive Tract
Authors: Brecchia Gabriele , Muça Gerald , Munga Albana , Menchetti Laura , Galosi Livio , Rossi Giacomo , Barbato Olimpia , Pastorelli Grazia , Agradi Stella , Serra Valentina , Sulçe Majlind , Ozuni Enkeleda , Turmalaj Luigj , Castrica Marta , Ceccarini Maria Rachele , Riva Federica , Fioretti Bernard , Quattrone Alda , Marongiu Maria Laura , Curone Giulio
Published: November 2023

Goji berry (GB) shows beneficial effects on human health, although its effects on the male rabbit have been little investigated. This study examines the impact of GB dietary supplementation on the semen traits, antioxidant capacity of seminal plasma, and histological features of the reproductive tract of rabbit buck. Eighteen rabbits were distributed into two dietary groups: one receiving a commercial feed (Control), and the other a feed supplemented with 1% of GB (Goji). After a nutritional adaptation period of 60 days, the animals were subjected to semen collection every 15 days. The semen traits, libido, antioxidant, and inflammatory parameters were collected and analyzed. The rabbits were sacrificed after 60 days, and tissues of the genital tract were analyzed. Compared to the Control group, the Goji group showed higher spermatozoa concentration, motility, and vitality (p < 0.05), as well as fewer abnormal spermatozoa and a higher libido (p < 0.1). Histological features such as functional activity and hyperplasia were improved by GB and correlated with some semen traits (p < 0.05). Conversely, antioxidant and anti-inflammatory parameters were unaffected by the diet. These findings suggest that GB acts on the tissues of the reproductive tract positively influencing semen quality, although further studies are needed to understand the effect on oxidative stress.
Removal of Antibiotics by Biochars: A Critical Review
Authors: Sen Umut , Esteves Bruno , Aguiar Terencio , Pereira Helena
Published: November 2023

Antibiotics are pharmaceuticals that are used to treat bacterial infections in humans and animals, and they are also used as growth promoters in livestock production. These activities lead to an alarming accumulation of antibiotics in aquatic environments, resulting in selection pressure for antibiotic resistance. Given that it is impractical to completely avoid the use of antibiotics, addressing the removal of antibiotics from the environment has become an important challenge. Adsorption methods and adsorbents have received particular attention because adsorption is highly efficient in the removal of low-concentration chemicals. Among the different adsorbents, biochars have shown promise for antibiotic removal, owing to their low cost and efficiency as well as their potential for modification to further increase their adsorption capacity. This review attempts to analyze the surface properties and ash contents of different biochars and to critically discuss the knowledge gaps in antibiotic adsorption. A total of 184 articles on antibiotic properties, adsorption of antibiotics, and biochar properties were reviewed, with a focus on the last 12 years. Antibiotic adsorption by pristine biochars and modified biochars was critically reviewed. Recommendations are provided for the adsorption of different antibiotic classes by biochars.
Evaluation and Projection of Gale Events in North China
Authors: Yuan Rong , Li Qiuyue , Wu Lingfang , Huo Miao , Huang Yi
Published: November 2023

As an important weather extreme, gales greatly impact the air quality, agriculture, aviation, and renewable energy in North China. However, the spatial–temporal changes in gale events remain unknown in North China. In this study, using the ERA5 reanalysis with high spatiotemporal resolution and multi-model simulations from the Coupled Model Intercomparison Project Phase Six (CMIP6), we investigate the temporal changes, including daily, seasonal and decadal variations in gale events in North China between 1980 and 2021, and we project the changes in gale events in the mid and late 21st century under two shared socio-economic pathways (SSPs). The gale events show large spatial heterogeneity in frequency, with a high frequency of >25 days/year in central Inner Mongolia province, northern Hebei province, and northwestern Beijing. Over the past four decades, the gale events in North China have shown a decadal reversal, with a decrease between 1980 and 2006 and an increase between 2007 and 2014. Furthermore, the gale events show strong temporal variations in North China. For seasonal variation, the gale events exhibit double peaks, with the largest peak in April and the smallest peak in October. For daily variation, the gale events show a single peak, with the maximum from 10:00 to 16:00 local time in North China. Multi-model simulations from CMIP6 reveal a continuous decreasing trend of gale events in North China by the end of the 21st century under both SSP1-2.6 and SSP2-4.5 scenarios relative to the historical period. Our results provide comprehensive support for planning aviation, renewable energy, and agriculture in the future.
Machine Learning Technology for EEG-Forecast of the Blood–Brain Barrier Leakage and the Activation of the Brain’s Drainage System during Isoflurane Anesthesia
Authors: Semyachkina-Glushkovskaya Oxana , Sergeev Konstantin , Semenova Nadezhda , Slepnev Andrey , Karavaev Anatoly , Hramkov Alexey , Prokhorov Mikhail , Borovkova Ekaterina , Blokhina Inna , Fedosov Ivan , Shirokov Alexander , Dubrovsky Alexander , Terskov Andrey , Manzhaeva Maria , Krupnova Valeria , Dmitrenko Alexander , Zlatogorskaya Daria , Adushkina Viktoria , Evsukova Arina , Tuzhilkin Matvey , Elizarova Inna , Ilyukov Egor , Myagkov Dmitry , Tuktarov Dmitry , Kurths Jürgen
Published: November 2023

Anesthesia enables the painless performance of complex surgical procedures. However, the effects of anesthesia on the brain may not be limited only by its duration. Also, anesthetic agents may cause long-lasting changes in the brain. There is growing evidence that anesthesia can disrupt the integrity of the blood–brain barrier (BBB), leading to neuroinflammation and neurotoxicity. However, there are no widely used methods for real-time BBB monitoring during surgery. The development of technologies for an express diagnosis of the opening of the BBB (OBBB) is a challenge for reducing post-surgical/anesthesia consequences. In this study on male rats, we demonstrate a successful application of machine learning technology, such as artificial neural networks (ANNs), to recognize the OBBB induced by isoflurane, which is widely used in surgery. The ANNs were trained on our previously presented data obtained on the sound-induced OBBB with an 85% testing accuracy. Using an optical and nonlinear analysis of the OBBB, we found that 1% isoflurane does not induce any changes in the BBB, while 4% isoflurane caused significant BBB leakage in all tested rats. Both 1% and 4% isoflurane stimulate the brain’s drainage system (BDS) in a dose-related manner. We show that ANNs can recognize the OBBB induced by 4% isoflurane in 57% of rats and BDS activation induced by 1% isoflurane in 81% of rats. These results open new perspectives for the development of clinically significant bedside technologies for EEG-monitoring of OBBB and BDS.
Adolescent Use of Dating Applications and the Associations with Online Victimization and Psychological Distress
Authors: Huang Tzu-Fu , Hou Chun-Yin , Chang Fong-Ching , Chiu Chiung-Hui , Chen Ping-Hung , Chiang Jeng-Tung , Miao Nae-Fang , Chuang Hung-Yi , Chang Yen-Jung , Chang Hsi , Chen Hsueh-Chih
Published: November 2023

In this study, we examined the relationships between the use of online dating applications (apps), online victimization, and psychosocial distress among adolescents. This study was conducted in 2020. A sample of 2595 seventh-grade students from 30 Taiwanese middle schools was surveyed. We conducted a self-administered survey. Overall, 15% of the adolescents reported using online dating apps in the past year, while 78% reported having seen dating app advertisements on the internet in the past year. Multivariate analysis results indicated that adolescents’ exposure to the marketing of dating apps and poor academic performance were both associated with the use of online dating apps. Adolescents who used dating apps were more likely to experience online privacy victimization, cyberbullying victimization, and online sexual harassment. The use of dating apps by adolescents, online privacy victimization, cyberbullying victimization, and online sexual harassment were associated with higher levels of depression, anxiety, and stress. In conclusion, adolescent use of dating apps is related to online victimization and psychological distress.
Thermo–Solid Coupling Analysis of Bionic Piston for a Mud Pump in Tunnel Engineering
Authors: Tian Hongjie , Tian Dongzhuang , Ju Pei , He Jinxuan
Published: November 2023

With the development of mud shield tunnel construction technology, the demands on the working performance of a mud pump are becoming higher and higher. As one of the critical components of a mud pump that is easy to wear, the performance of the piston directly affects the operational efficiency and lifespan of the mud pump. The bionic shape of the piston was designed under the guidance of non-smooth surface characteristics of natural organisms to enhance friction and wear performance as well as longevity. The stress field and temperature field characteristics of the pistons with three bionic structures (pit, stripe, and prismatic) were analyzed based on finite element simulation. The stress field analysis results indicated that, for the prismatic shape and pit shape pistons, the maximum stress was concentrated in the lip regions, and both of them bore large stress at the root. For the stripe-shaped piston, the stress was dispersed on both sides of the stripe structure, the stress at the root was small, and the stress gradient along the axial direction was relatively gentle. The stripe-shaped bionic structure can significantly improve the stress distribution state on the piston surface, and the optimal stripe width was recommended to be between 1 and 1.5 mm. The temperature field analysis results indicated that, for the stripe-shaped piston, the surface temperature and heat flux were the smallest, and the temperature gradient was relatively smaller than that of pit-shaped and prismatic-shaped pistons, so it was easier to dissipate heat. When the stripe width was 1.5 mm, the temperature distribution was the most uniform, and the heat flux in localized areas was the smallest, so the heat generated by friction was relatively easy to discharge in the unit area.
Baseline Values of Circulating IL-6 and TGF-β Might Identify Patients with HNSCC Who Do Not Benefit from Nivolumab Treatment
Authors: Merlano Marco Carlo , Paccagnella Matteo , Denaro Nerina , Abbona Andrea , Galizia Danilo , Sangiolo Dario , Gammaitoni Loretta , Fiorino Erika , Minei Silvia , Bossi Paolo , Licitra Lisa , Garrone Ornella
Published: November 2023

Background: The immunotherapy of head and neck cancer induces a limited rate of long-term survivors at the cost of treating many patients exposed to toxicity without benefit, regardless of PD-L1 expression. The identification of better biomarkers is warranted. We analyzed a panel of cytokines, chemokines and growth factors, hereinafter all referred to as ‘cytokines’, as potential biomarkers in patients with head and neck cancer treated with nivolumab. Materials and methods: A total of 18 circulating cytokines were analyzed. Samples were gathered at baseline (T0) and after 3 courses of nivolumab (T1) in patients with relapsed/metastatic disease. The data extracted at T0 were linked to survival; the comparison of T0–T1 explored the effect of immunotherapy. Results: A total of 22 patients were accrued: 64% current heavy smokers, 36% female and 14% had PS = 2. At T0, ROC analysis showed that IL-6, IL-8, IL-10 and TGF-β were higher in patients with poor survival. Cox analysis demonstrated that only patients with the IL-6 and TGF-β discriminate had good or poor survival, respectively. Longitudinal increments of CCL-4, IL-15, IL-2 and CXCL-10 were observed in all patients during nivolumab treatment. Conclusion: In this small population with poor clinical characteristics, this study highlights the prognostic role of IL-6 and TGF-β. Nivolumab treatment is associated with a positive modulation of some Th1 cytokines, but it does not correlate with the outcome.
MicroRNA Landscape in Endometrial Carcinomas in an Asian population: Unraveling Subtype-Specific Signatures
Authors: Tan Gideon Ze Lin , Leong Sai Mun , Jin Yu , Kuick Chik Hong , Chee Jeremy Joon Keat , Low San Zeng , Ding Ling-Wen , Cheng He , Lim Diana , Hue Susan Swee-Shan
Published: November 2023

MicroRNAs (MiRNAs) are small, non-coding RNA molecules that function in RNA silencing and post-transcriptional regulation of gene expression. We analyzed the differential expression of miRNAs in 119 endometrial carcinomas, measuring their expression in histological subtypes, molecular subtypes, and tumors with CTNNB1 mutations. Tumors were subdivided into histological and molecular subtypes as defined by The Cancer Genome Atlas. The expression levels of 352 miRNAs were quantified using the PanoramiR panel. Mir-449a, mir-449b-5p, and mir-449c-5p were the top three miRNAs showing increased expression in both endometrioid and de-differentiated carcinomas but were not significantly increased in serous and clear cell carcinomas. The miRNAs with the most increased expression in serous and clear cell carcinomas were miR-9-3p and miR-375, respectively. We also identified 62 differentially expressed miRNAs among different molecular subtypes. Using sequential forward selection, we built subtype classification models for some molecular subtypes of endometrial carcinoma, comprising 5 miRNAs for MMR-deficient tumors, 10 miRNAs for p53-mutated tumors, and 3 miRNAs for CTNNB1-mutated tumors, with areas under curves of 0.75, 0.85, and 0.78, respectively. Our findings confirm the differential expression of miRNAs between various endometrial carcinoma subtypes and may have implications for the development of diagnostic and prognostic tools.