bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish
Authors: Khatib Ihab , Horyn Oksana , Bodnar Oksana , Lushchak Oleh , Rychter Piotr , Falfushynska Halina
Published: March 2023

Our research sought to determine the molecular and biochemical effects of environmentally relevant exposure to commonly used chloro-s-triazine herbicide terbuthylazine and organophosphate insecticide malathion on zebrafish. To this aim, mature zebrafish were exposed to 2 and 30 µg L−1 terbuthylazine and 5 and 50 µg L−1 malathion alone and in combination for 14 days. Aside from the accumulation of TBARS and protein carbonyls, a decrease in antioxidants and succinate dehydrogenase activity, an increase in oxidized glutathione, and enhanced apoptosis via Caspase-3 and BAX overexpression were observed. Furthermore, terbuthylazine and malathion induced mitochondrial swelling (up to 210% after single exposure and up to 470% after co-exposure) and lactate dehydrogenase leakage (up to 268% after single exposure and up to 570% after co-exposure) in a concentration-dependent manner. Significant upregulation of ubiquitin expression and increased cathepsin D activity were characteristics that appeared only upon terbuthylazine exposure, whereas the induction of IgM was identified as the specific characteristic of malathion toxicity. Meanwhile, no alterations in the zebrafish hypothalamic-pituitary-thyroid axis was observed. Co-exposure increased the adverse effects of individual pesticides on zebrafish. This study should improve the understanding of the mechanisms of pesticide toxicity that lead to fish impairment and biodiversity decline.
Double-Fermented Soybean Meal Totally Replaces Soybean Meal in Broiler Rations with Favorable Impact on Performance, Digestibility, Amino Acids Transporters and Meat Nutritional Value
Authors: Abdel-Raheem Sherief M. , Mohammed El Said Yehia , Mahmoud Rania Elsaid , El Gamal Mahmoud Fathy , Nada Hend S. , El-Ghareeb Waleed Rizk , Marzok Mohamed , Meligy Ahmed M. A. , Abdulmohsen Mohamad , Ismail Hesham , Ibrahim Doaa , Kishawy Asmaa T. Y.
Published: March 2023

Inclusion of microbial fermented soybean meal in broiler feed has induced advantageous outcomes for their performance and gastrointestinal health via exhibiting probiotic effects. In this study, soybean meal (SBM) was subjected to double-stage microbial fermentation utilizing functional metabolites of fungi and bacteria. In broiler diet, DFSBM replaced SBM by 0, 25, 50 and 100%. DFSBM was reported to have higher protein content and total essential, nonessential and free amino acids (increased by 3.67%, 12.81%, 10.10% and 5.88-fold, respectively, compared to SBM). Notably, phytase activity and lactic acid bacteria increased, while fiber, lipid and trypsin inhibitor contents were decreased by 14.05%, 38.24% and 72.80%, respectively, in a diet containing 100% DFSBM, compared to SBM. Improved growth performance and apparent nutrient digestibility, including phosphorus and calcium, and pancreatic digestive enzyme activities were observed in groups fed higher DFSBM levels. In addition, higher inclusion levels of DFSBM increased blood immune response (IgG, IgM, nitric oxide and lysozyme levels) and liver antioxidant status. Jejunal amino acids- and peptide transporter-encoding genes (LAT1, CAT-1, CAT-2, PepT-1 and PepT-2) were upregulated with increasing levels of DFSBM in the ration. Breast muscle crude protein, calcium and phosphorus retention were increased, especially at higher inclusion levels of DFSBM. Coliform bacteria load was significantly reduced, while lactic acid bacteria count in broiler intestines was increased with higher dietary levels of DFSBM. In conclusion, replacement of SBM with DFSBM positively impacted broiler chicken feed utilization and boosted chickens’ amino acid transportation, in addition to improving the nutritional value of their breast meat.
Dairy Cows Are Limited in Their Ability to Increase Glucose Availability for Immune Function during Disease
Authors: Habel Jonas , Sundrum Albert
Published: March 2023

Shortages of energy and glucose have been hypothesized to play a key role in the development of and responses to production diseases in dairy cows during early lactation. Given the importance of glucose for immune functions, we used a recently established method for the estimation of glucose balance (GB) to evaluate glucose availability during disease phases. A dataset comprising ration analyses as well as individual daily milk yields (MY), dry matter intake (DMI), body weights, and health records of 417 lactations (298 cows) was used to calculate individual daily GB and energy balance (EB). The magnitude and dynamics of MY, DMI, GB, and EB were evaluated in the weeks before, at, and after diagnoses of inflammatory diseases in different stages of early lactation from week in milk 1 to 15. Diagnoses were categorized as mastitis, claw and leg diseases, and other inflammatory diseases. Mixed linear models with a random intercept and slope term for each lactation were used to evaluate the effect of diagnosis on MY, DMI, GB, and EB while accounting for the background effects of week in milk, parity, season, and year. When unaffected by disease, in general, the GB of cows was close to zero in the first weeks of lactation and increased as lactation progressed. Weekly means of EB were negative throughout all lactation stages investigated. Disease decreased both the input of glucose precursors due to a reduced DMI as well as the output of glucose via milk due to a reduced MY. On average, the decrease in DMI was −1.5 (−1.9 to −1.1) kg and was proportionally higher than the decrease in MY, which averaged −1.0 (−1.4 to −0.6) kg. Mastitis reduced yield less than claw and leg disease or other diseases. On average, GB and EB were reduced by −3.8 (−5.6 to −2.1) mol C and −7.5 (−10.2 to −4.9) MJ in the week of diagnosis. This indicates the need to investigate strategies to increase the availability of glucogenic carbon for immune function during disease in dairy cows.
Microbial Contamination and Antibiotic Resistance in Marketed Food in Bangladesh: Current Situation and Possible Improvements
Authors: Samad Mohammed Abdus , Eberson Linnea , Begum Ruhena , Alam Mohammad Gazi Shah , Talukdar Faisol , Akter Rahima , Dang-Xuan Sinh , Sharma Garima , Islam Shariful , Siddiky Nure Alam , Uddin ASM Ashab , Asheak Mahmud Mohammad , Sarker Md Samun , Rahman Md. Siddiqur , Grace Delia , Lindahl Johanna F.
Published: March 2023

Antimicrobial resistance (AMR) is a public health problem worldwide. Bangladesh, like its neighboring countries, faces many public health challenges, including access to safe food, inadequate food surveillance, as well as increasing AMR. This study investigated bacterial contamination and the AMR profile of pathogens in marketed food in Bangladesh and explored barriers to reducing AMR in the country. We collected 366 tomatoes, 359 chicken and 249 fish samples from 732 vendors in traditional markets in urban, peri-urban and rural areas in Bangladesh, as well as from 121 modern retails in Dhaka capital to analyse Vibrio cholerae and Escherichia coli in fish, Salmonella in chicken, and Salmonella and E. coli in tomatoes. Antibiotic susceptibility against 11 antibiotics was tested using a disc diffusion test and interpreted by an automated zone inhibition reader. In addition, a qualitative study using key informant interviews was conducted to explore antimicrobial use and AMR reduction potential in Bangladesh. We found E. coli in 14.21% of tomatoes and 26.91% of fish samples, while 7.38% of tomatoes and 17.27% of chicken were positive for Salmonella, and 44.98% of fish were positive for Vibrio cholerae. In total 231/319 (72.4%) of all pathogens isolated were multidrug-resistant (MDR) (resistant to three or more antibiotic groups). Qualitative interviews revealed an inadequate surveillance system for antibiotic use and AMR in Bangladesh, especially in the agriculture sector. To be able to fully understand the human health risks from bacterial hazards in the food and the AMR situation in Bangladesh, a nationwide study with a one health approach should be conducted, within all sectors, including AMR testing as well as assessment of the antimicrobial use and its drivers.
Knowledge, Perceptions, and Perspectives of Medical Students Regarding the Use of Antibiotics and Antibiotic Resistance: A Qualitative Research in Galicia, Spain
Authors: Vázquez-Lago Juan M. , Montes-Villalba Rodrigo A. , Vázquez-Cancela Olalla , Otero-Santiago María , López-Durán Ana , Figueiras Adolfo
Published: March 2023

Antibiotic resistance is a significant public health concern, with numerous studies linking antibiotic consumption to the development of resistance. As medical students will play a pivotal role in prescribing antibiotics, this research aimed to identify their perceptions of current use and factors that could influence future inappropriate use of antibiotics. The study employed a qualitative research approach using Focus Group discussions (FGs) consisting of students from the final theoretical course of the Medicine degree. The FGs were conducted based on a pre-script developed from factors contributing to antibiotic misuse identified in previous studies. All sessions were recorded and transcribed for analysis by two independent researchers, with all participants signing informed consent. Seven focus groups were conducted, with a total of 35 participants. The study identified factors that could influence the future prescription of antibiotics, including the low applicability of knowledge, insecurity, clinical inertia, difficulties in the doctor-patient relationship, unawareness of available updates on the topic, and inability to assess their validity. The students did not perceive antibiotic resistance as a current problem. However, the study found several modifiable factors in medical students that could explain the misuse of antibiotics, and developing specific strategies could help improve their use.
Novel Anti-Acanthamoebic Activities of Irosustat and STX140 and Their Nanoformulations
Authors: Siddiqui Ruqaiyyah , Rawas-Qalaji Mutasem , El-Gamal Mohammed I. , Sajeev Sreedevi , Jagal Jayalakshmi , Zaraei Seyed-Omar , Sbenati Rawan M. , Anbar Hanan S. , Dohle Wolfgang , Potter Barry V. L. , Khan Naveed Ahmed
Published: March 2023

Pathogenic Acanthamoeba produce keratitis and fatal granulomatous amoebic encephalitis. Treatment remains problematic and often ineffective, suggesting the need for the discovery of novel compounds. For the first time, here we evaluated the effects of the anticancer drugs Irosustat and STX140 alone, as well as their nanoformulations, against A. castellanii via amoebicidal, excystment, cytopathogenicity, and cytotoxicity assays. Nanoformulations of the compounds were successfully synthesized with high encapsulation efficiency of 94% and 82% for Irosustat and STX140, respectively. Nanoparticles formed were spherical in shape and had a unimodal narrow particle size distribution, mean of 145 and 244 nm with a polydispersity index of 0.3, and surface charge of −14 and −15 mV, respectively. Irosustat and STX140 exhibited a biphasic release profile with almost 100% drug released after 48 h. Notably, Irosustat significantly inhibited A. castellanii viability and amoebae-mediated cytopathogenicity and inhibited the phenotypic transformation of amoebae cysts into the trophozoite form, however their nanoformulations depicted limited effects against amoebae but exhibited minimal cytotoxicity when tested against human cells using lactate dehydrogenase release assays. Accordingly, both compounds have potential for further studies, with the hope of discovering novel anti-Acanthamoeba compounds, and potentially developing targeted therapy against infections of the central nervous system.
Microbiological Retention on PTFE versus Silk Suture: A Quantitative Pilot Study in Third Molar Surgery
Authors: Parrini Stefano , Bovicelli Alessandro , Chisci Glauco
Published: March 2023

Background: Mandibular third molar (M3M) removal and management of postoperative complications represent a common matter of interest in oral and maxillofacial surgery. This potential quantitative study assessed the ability of two types of surgical sutures, Silk and polytetrafluoroethylene polymer (PTFE), to carry aerobic and anaerobic bacteria on wounds after mandibular third molar surgery, with a collection of the stitches at the suture removal and study in the laboratory on the basis of colony-forming units. Methods: This prospective quantitative study sampled a total of 10 consecutive healthy patients for mandibular third molar surgery at the Oral Surgery School, Dentistry and Dental Prosthodontics, Department of Medical Biotechnologies, University of Siena, Siena, Italy. The mean age of the patients was 31 years (range 25–40 years), seven patients were male and three patients were female. Inclusion criteria were: presence of a partially impacted mandibular third molar. Exclusion criteria were: smoking and diabetes mellitus. Extraction of the mandibular third molar was performed under local anesthesia: after the third molar surgery, two sutures were applied on the surgical site distally to the second mandibular molar: one single 3/0 silk stitch; one single 3/0 PTFE stitch. No sutures were applied on the release incision. Sutures were removed after 7 days and were immediately conserved and sent to the laboratory to be rated on the basis of colony-forming units (CFUs). CFUs were evaluated and reported on GraphPad Prism and transformed into its base 10 logarithm. Data were analyzed with a non-parametric Wilcoxon test, and p-values < 0.05 were evaluated as statistically significant. Results: All the patients attended the suture removal date, and all the sutures were present in the site. None of the surgical sites presented dehiscence. No stitch loss was reported, and no patient reported mouth washing or tooth brushing in the surgery site. All interventions were uneventful and no major complications were reported after M3M surgery. Bacterial retention resulted as statistically greater in silk sutures rather than PTFE sutures, both in Brain Heart Infusion samples (p = 0.003) and Wilkins-Chalgren anaerobe samples (p = 0.002). Conclusions: We found the PTFE suture to be superior to the silk suture in a reduction in the bacterial biofilm in both aerobic and anaerobic evaluations after M3M surgery.
Manipulation of HSP70-SOD1 Expression Modulates SH-SY5Y Differentiation and Susceptibility to Oxidative Stress-Dependent Cell Damage: Involvement in Oxotremorine-M-Mediated Neuroprotective Effects
Authors: Scordino Miriana , Frinchi Monica , Urone Giulia , Nuzzo Domenico , Mudò Giuseppa , Di Liberto Valentina
Published: March 2023

The differentiation of neural progenitors is a complex process that integrates different signals to drive transcriptional changes, which mediate metabolic, electrophysiological, and morphological cellular specializations. Understanding these adjustments is essential within the framework of stem cell and cancer research and therapy. Human neuroblastoma SH-SY5Y cells, widely used in neurobiology research, can be differentiated into neuronal-like cells through serum deprivation and retinoic acid (RA) supplementation. In our study, we observed that the differentiation process triggers the expression of Heat Shock Protein 70 (HSP70). Notably, inhibition of HSP70 expression by KNK437 causes a dramatic increase in cell death. While undifferentiated SH-SY5Y cells show a dose-dependent decrease in cell survival following exposure to hydrogen peroxide (H2O2), differentiated cells become resistant to H2O2-induced cell death. Interestingly, the differentiation process enhances the expression of SOD1 protein, and inhibition of HSP70 expression counteracts this effect and increases the susceptibility of differentiated cells to H2O2-induced cell death, suggesting that the cascade HSP70-SOD1 is involved in promoting survival against oxidative stress-dependent damage. Treatment of differentiated SH-SY5Y cells with Oxotremorine-M (Oxo), a muscarinic acetylcholine receptor agonist, enhances the expression of HSP70 and SOD1 and counteracts tert–Butyl hydroperoxide-induced cell death and reactive oxygen species (ROS) generation. It is worth noting that co-treatment with KNK437 reduces SOD1 expression and Oxo-induced protection against oxidative stress damage, suggesting the involvement of HSP70/SOD1 signaling in this beneficial effect. In conclusion, our findings demonstrate that manipulation of the HSP70 signal modulates SH-SY5Y differentiation and susceptibility to oxidative stress-dependent cell death and unravels novel mechanisms involved in Oxo neuroprotective functions. Altogether these data provide novel insights into the mechanisms underlying neuronal differentiation and preservation under stress conditions.
Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity
Authors: Wang Likai , Yan Laiqing , Han Qi , Li Guangdong , Wu Hao , Ma Xiao , Zhao Mengmeng , Ma Wenkui , Ji Pengyun , Zhang Ran , Liu Guoshi
Published: March 2023

In this study, the effects of daily melatonin supplementation (2 mg/kg) at the late gestational stage on the reproductive performance of the sows have been investigated. This treatment potentially increased the litter size and birth survival rate and significantly increased the birth weight as well as the weaning weight and survival rate of piglets compared to the controls. The mechanistic studies have found that these beneficial effects of melatonin are not mediated by the alterations of reproductive hormones of estrogen and progesterone, nor did the glucose and lipid metabolisms, but they were the results of the reduced oxidative stress in placenta associated with melatonin supplementation. Indeed, the melatonergic system, including mRNAs and proteins of AANAT, MTNR1A and MTNR1B, has been identified in the placenta of the sows. The RNA sequencing of placental tissue and KEGG analysis showed that melatonin activated the placental tissue fluid shear stress pathway to stimulate the Nrf2 signaling pathway, which upregulated its several downstream antioxidant genes, including MGST1, GSTM3 and GSTA4, therefore, suppressing the placental oxidative stress. All these actions may be mediated by the melatonin receptor of MTNR1B.
A Sex-Specific Comparative Analysis of Oxidative Stress Biomarkers Predicting the Risk of Cardiovascular Events and All-Cause Mortality in the General Population: A Prospective Cohort Study
Authors: Bourgonje Martin F. , Abdulle Amaal E. , Kieneker Lyanne M. , la Bastide-van Gemert Sacha , Bakker Stephan J. L. , Gansevoort Ron T. , Gordijn Sanne J. , van Goor Harry , Bourgonje Arno R.
Published: March 2023

Oxidative stress plays a pivotal role in cardiovascular (CV) disease, but current biomarkers used to predict CV events are still insufficient. In this study, we comparatively assessed the utility of redox-related biomarkers in predicting the risk of CV events and all-cause mortality in male and female subjects from the general population. Subjects (n = 5955) of the Prevention of REnal and Vascular ENd-stage Disease (PREVEND) population-based cohort study were included. Blood homocysteine, gamma-GT, HDL cholesterol, bilirubin and protein-adjusted free thiol (R-SH, sulfhydryl groups) levels were quantified at baseline and were prospectively analyzed in association with the risk of CV events and all-cause mortality. After adjustment for potentially confounding factors, protein-adjusted R-SH and homocysteine levels were significantly associated with the risk of CV events in men (HR 0.63 [0.40–0.99], p = 0.045 and HR 1.58 [1.20–2.08], p = 0.001, respectively). Protein-adjusted R-SH and HDL cholesterol levels were significantly associated with the risk of all-cause mortality in men (HR 0.52 [0.32–0.85], p = 0.009 and HR 0.90 [0.85–0.94], p < 0.001, respectively), while the same was observed for bilirubin and homocysteine levels in women (HR 0.68 [0.48–0.98], p = 0.040 and HR 2.30 [1.14–3.76], p < 0.001, respectively). Lower levels of protein-adjusted R-SH were robustly associated with an increased risk of CV events and all-cause mortality in men. Our results highlight the value of R-SH levels in cardiovascular risk assessment and their potential significance as being amenable to therapeutic intervention, while reaffirming the importance of other oxidative stress-related biomarkers, such as homocysteine, HDL cholesterol and bilirubin.
Transcriptional Inhibition of AGPAT2 Induces Abnormal Lipid Metabolism and Oxidative Stress in the Liver of Nile Tilapia Oreochromis niloticus
Authors: Feng Tiantian , Tao Yifan , Yan Yue , Lu Siqi , Li Yan , Zhang Xing , Qiang Jun
Published: March 2023

The enzyme 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) is an intermediate enzyme in triglyceride synthesis. The aim was to study the regulatory mechanism of AGPAT2 on Nile tilapia, Oreochromis niloticus. In this study, antisense RNA technology was used to knock-down AGPAT2 in Nile tilapia. Compared with the control groups (transfected with ultrapure water or the blank expression vector), the AGPAT2 knock-down group showed a significantly higher weight gain rate, special growth rate, visceral somatic index, and hepatopancreas somatic index; and significantly increased the total cholesterol, triglycerides, glucose, low-density lipoprotein cholesterol, and insulin levels in serum. In addition, the contents of total cholesterol and triglycerides and the abundance of superoxide dismutase, catalase, and glutathione peroxidase in the liver significantly increased, while the malondialdehyde content significantly decreased. The liver cells became severely vacuolated and accumulated lipids in the AGPAT2 knock-down group. Comparative transcriptome analyses (AGPAT2 knock-down vs. control group) revealed 1789 differentially expressed genes (DEGs), including 472 upregulated genes and 1313 downregulated genes in the AGPAT2 knock-down group. Functional analysis showed that the main pathway of differentially expressed genes enrichment was lipid metabolism and oxidative stress, such as steroid biosynthesis, unsaturated fatty acid biosynthesis, the PPAR signaling pathway, and the P53 pathway. We used qRT-PCR to verify the mRNA expression changes of 13 downstream differential genes in related signaling pathways. These findings demonstrate that knock-down of AGPAT2 in tilapia leads to abnormal lipid metabolism and oxidative stress.
Ceruloplasmin Interferes with the Assessment of Blood Lipid Hydroperoxide Content in Small Ruminants
Authors: Cecchini Gualandi Stefano , Boni Raffaele
Published: March 2023

Simple and inexpensive analytical methods for assessing redox balance in biological matrixes are widely used in animal and human diagnostics. Two of them, reactive oxygen metabolites (ROMs) and total oxidant status (TOS), evaluate the lipid hydroperoxide (LOOH) content of the sample and are based on iron-mediated mechanisms. However, these tests provide uncorrelated results. In this study, we compared these two tests in the blood serum of goat kids and lambs, together with an evaluation of ceruloplasmin (CP) oxidase activity. No significant correlation was found between ROMs and TOS, or between TOS and CP oxidase activity, in either species. Conversely, ROMs and CP oxidase activity were highly correlated in both kid and lamb samples (p < 0.001). A significant progressive reduction in the analytical signal in the ROMs assay was observed when sodium azide, an effective CP inhibitor, was added to the samples before the assay (p < 0.001). This decrease was related to sodium azide concentration (p < 0.01) and was not found when sodium azide was added at the same concentrations in the TOS assay. These findings suggest that ROMs, unlike TOS, may be affected by CP, which interferes with LOOH detection in blood samples.
Different Effects and Mechanisms of Selenium Compounds in Improving Pathology in Alzheimer’s Disease
Authors: Zhang Zhong-Hao , Peng Jia-Ying , Chen Yu-Bin , Wang Chao , Chen Chen , Song Guo-Li
Published: March 2023

Owing to the strong antioxidant capacity of selenium (Se) in vivo, a variety of Se compounds have been shown to have great potential for improving the main pathologies and cognitive impairment in Alzheimer’s disease (AD) models. However, the differences in the anti-AD effects and mechanisms of different Se compounds are still unclear. Theoretically, the absorption and metabolism of different forms of Se in the body vary, which directly determines the diversification of downstream regulatory pathways. In this study, low doses of Se-methylselenocysteine (SMC), selenomethionine (SeM), or sodium selenate (SeNa) were administered to triple transgenic AD (3× Tg-AD) mice for short time periods. AD pathology, activities of selenoenzymes, and metabolic profiles in the brain were studied to explore the similarities and differences in the anti-AD effects and mechanisms of the three Se compounds. We found that all of these Se compounds significantly increased Se levels and antioxidant capacity, regulated amino acid metabolism, and ameliorated synaptic deficits, thus improving the cognitive capacity of AD mice. Importantly, SMC preferentially increased the expression and activity of thioredoxin reductase and reduced tau phosphorylation by inhibiting glycogen synthase kinase-3 beta (GSK-3β) activity. Glutathione peroxidase 1 (GPx1), the selenoenzyme most affected by SeM, decreased amyloid beta production and improved mitochondrial function. SeNa improved methionine sulfoxide reductase B1 (MsrB1) expression, reflected in AD pathology as promoting the expression of synaptic proteins and restoring synaptic deficits. Herein, we reveal the differences and mechanisms by which different Se compounds improve multiple pathologies of AD and provide novel insights into the targeted administration of Se-containing drugs in the treatment of AD.
Metal-Based Nanoparticles and Their Relevant Consequences on Cytotoxicity Cascade and Induced Oxidative Stress
Authors: Min Yunhui , Suminda Godagama Gamaarachchige Dinesh , Heo Yunji , Kim Mangeun , Ghosh Mrinmoy , Son Young-Ok
Published: March 2023

Emerging nanoscience allows us to take advantage of the improved evolutionary components and apply today’s advanced characterization and fabrication techniques to solve environmental and biological problems. Despite the promise that nanotechnology will improve our lives, the potential risks of technology remain largely uncertain. The lack of information on bio-impacts and the absence of consistent standards are the limitations of using metal-based nanoparticles (mNPs) for existing applications. To analyze the role played by the mNPs physicochemical characteristics and tactics to protect live beings, the field of nanotoxicology nowadays is focused on collecting and analyzing data from in vitro and in vivo investigations. The degree of reactive oxygen species (ROS) and oxidative stress caused by material nanoparticles (NPs) depends on many factors, such as size, shape, chemical composition, etc. These characteristics enable NPs to enter cells and interact with biological macromolecules and cell organelles, resulting in oxidative damage, an inflammatory response, the development of mitochondrial dysfunction, damage to genetic material, or cytotoxic effects. This report explored the mechanisms and cellular signaling cascades of mNPs-induced oxidative stress and the relevant health consequences.
Potential Role of Natural Antioxidant Products in Oncological Diseases
Authors: Marino Pasquale , Pepe Giacomo , Basilicata Manuela Giovanna , Vestuto Vincenzo , Marzocco Stefania , Autore Giuseppina , Procino Alfredo , Gomez-Monterrey Isabel Maria , Manfra Michele , Campiglia Pietro
Published: March 2023

Nutrition has a significant effect and a crucial role in disease prevention. Low consumption of fruit and vegetables and a sedentary lifestyle are closely related with the onset and development of many types of cancer. Recently, nutraceuticals have gained much attention in cancer research due to their pleiotropic effects and relatively non-toxic behavior. In fact, although in the past there have been conflicting results on the role of some antioxidant compounds as allies against cancer, numerous recent clinical studies highlight the efficacy of dietary phytochemicals in the prevention and treatment of cancer. However, further investigation is necessary to gain a deeper understanding of the potential anticancer capacities of dietary phytochemicals as well as the mechanisms of their action. Therefore, this review examined the current literature on the key properties of the bioactive components present in the diet, such as carotenoids, polyphenols, and antioxidant compounds, as well as their use in cancer therapy. The review focused on potential chemopreventive properties, evaluating their synergistic effects with anticancer drugs and, consequently, the side effects associated with current cancer treatments.
Expression of the H2O2 Biosensor roGFP-Tpx1.C169S in Fission and Budding Yeasts and Jurkat Cells to Compare Intracellular H2O2 Levels, Transmembrane Gradients, and Response to Metals
Authors: de Cubas Laura , Mallor Jorge , Herrera-Fernández Víctor , Ayté José , Vicente Rubén , Hidalgo Elena
Published: March 2023

Intracellular hydrogen peroxide (H2O2) levels can oscillate from low, physiological concentrations, to intermediate, signaling ones, and can participate in toxic reactions when overcoming certain thresholds. Fluorescent protein-based reporters to measure intracellular H2O2 have been developed in recent decades. In particular, the redox-sensitive green fluorescent protein (roGFP)-based proteins fused to peroxiredoxins are among the most sensitive H2O2 biosensors. Using fission yeast as a model system, we recently demonstrated that the gradient of extracellular-to-intracellular peroxides through the plasma membrane is around 300:1, and that the concentration of physiological H2O2 is in the low nanomolar range. Here, we have expressed the very sensitive probe roGFP2-Tpx1.C169S in two other model systems, budding yeast and human Jurkat cells. As in fission yeast, the biosensor is ~40–50% oxidized in these cell types, suggesting similar peroxide steady-state levels. Furthermore, probe oxidation upon the addition of extracellular peroxides is also quantitatively similar, suggesting comparable plasma membrane H2O2 gradients. Finally, as a proof of concept, we have applied different concentrations of zinc to all three model systems and have detected probe oxidation, demonstrating that an excess of this metal can cause fluctuations of peroxides, which are moderate in yeasts and severe in mammalian cells. We conclude that the principles governing H2O2 fluxes are very similar in different model organisms.
The Combination of Baicalein and Memantine Reduces Oxidative Stress and Protects against β-amyloid-Induced Alzheimer’s Disease in Rat Model
Authors: Jadhav Ratnakar , Kulkarni Yogesh A.
Published: March 2023

Alzheimer’s disease (AD) is a neuronal condition causing progressive loss of memory and cognitive dysfunction particularly in elders. An upsurge in the global old age population has led to a proportionate increase in the prevalence of AD. The current treatments for AD are symptomatic and have debilitating side effects. A literature review and current research have directed scientists to explore natural products with better safety and efficacy profiles as new treatment options for AD. Baicalein, belonging to the flavone subclass of flavonoids, has been reported for its anti-oxidant, anti-inflammatory, AChE enzyme inhibitory activity and anti-amyloid protein aggregation activity, which collectively demonstrates its benefits as a neuroprotective agent. Presently, memantine, a NMDAR antagonist, is one of the important drugs used for treatment of Alzheimer’s disease. The current study aims to investigate the effect of baicalein in combination with memantine in β-amyloid-induced AD in albino Wistar rats. Baicalein (10 mg/kg) alone, 5 mg/kg and 10 mg/kg in combination with memantine (20 mg/kg) was administered for 21 days. Treatment with baicalein in combination with memantine showed significant improvement in behavioural studies. The combination treatment decreased oxidative stress, β-amyloid plaque formation and increased the expression of brain-derived neurotrophic factor (BDNF) in the brain. From the results, it can be concluded that treatment with baicalein and memantine could be beneficial for reducing the progression of neurodegeneration in rats. Baicalein has an additive effect in combination with memantine, making it a potential option for the treatment of AD.
Dual Role of DUOX1-Derived Reactive Oxygen Species in Melanoma
Authors: Pardo-Sánchez Irene , Ibañez-Molero Sofía , García-Moreno Diana , Mulero Victoriano
Published: March 2023

Melanoma is the most serious type of skin cancer. Inflammation and oxidative stress play an essential role in the development of several types of cancer, including melanoma. Although oxidative stress promotes tumor growth, once cells escape from the primary tumor, they are subjected to a more hostile environment, with higher levels of oxidative stress typically killing most cancer cells. As Dual Oxidase 1 (DUOX1) is a major producer of reactive oxygen species (ROS) in epithelia, we used allotransplantation and autochthonous melanoma models in zebrafish together with in silico analysis of the occurrence and relevance of DUOX1 expression of the skin cutaneous melanoma (SKCM) cohort of The Cancer Genome Atlas (TCGA) to address the role of this enzyme in the aggressiveness of melanoma cells in vivo. It was found that high transcript levels of the gene encoding DUOX1 were associated with the poor prognosis of patients in the early-stage melanoma of TCGA cohort. However, DUOX1 transcript levels were not found to be associated to the prognosis of late-stage SKCM patients. In addition, the transcript level of DUOX1 in metastatic SKCM was lower than in primary SKCM. Using zebrafish primary melanoma and allotransplantation models, we interrogated the role of DUOX1 in vivo. Our results confirmed a dual role of DUOX1, which restrains melanoma proliferation but promotes metastasis. As this effect is only observed in immunocompromised individuals, the immune system appears to be able to counteract this elevated metastatic potential of DUOX1-deficient melanomas.
Effect of Rearing in Small-Cell Combs on Activities of Catalase and Superoxide Dismutase and Total Antioxidant Capacity in the Hemolymph of Apis mellifera Workers
Authors: Dziechciarz Piotr , Strachecka Aneta , Borsuk Grzegorz , Olszewski Krzysztof
Published: March 2023

Honeybee nests constructed without man-made wax foundation have significantly more variability of cell widths/sizes than those in commercially-kept colonies. The effects of this natural variability in comb cell widths on individual and colony traits have not been explained to date. The investigation of this problem can lead to new findings about the biology, physiology, and possibly, the evolution of the honeybee. The aim of the study was to compare the catalase and superoxide dismutase activities and the total antioxidant capacity levels in the hemolymph of honeybee workers reared in small-cell combs and standard-cell combs in colonies kept simultaneously on standard- and small-cell combs. The ratio of the small-cell combs to the standard-cell combs in the nest was 1:1. The workers reared in small-cell combs were characterized by higher antioxidant activities in the hemolymph than those reared in standard-cell combs. Consequently, their hemolymph had a greater antioxidant capacity, which indicates that they may be better predisposed to be foragers than workers reared in standard-cell combs. To describe the physiological differences between worker bees reared in small- and standard-cell combs in the same colony, the role of the considerable variation in the cell width in natural combs built without the use of artificially produced wax foundation is worth elucidating. The comparison of the apiary and cage experiments indicated that changes in antioxidant activities predominantly result from worker activities, especially those requiring the intensification of metabolism, rather than the age of the worker bees. To reduce the impact on the results of random environmental factors potentially present in one-season studies of honeybee research, investigations should preferably be carried out over a few consecutive years.
Influence of Microwave Radiation on Pollutant Removal and Biomethane Production Efficiency in Anaerobic Treatment of High-Load Poultry Wastewater
Authors: Zieliński Marcin , Dębowski Marcin , Rusanowska Paulina , Kazimierowicz Joanna
Published: March 2023

The growing consumption of poultry meat has spurred the development of meat-processing plants and an associated rise in wastewater generation. Anaerobic digestion is one of the preferred processes for treating such waste. The current push towards biogas upgrading and out-of-plant use necessitates new, competitive ways of heating digesters. One such alternative is to use electromagnetic microwave radiation (EMR). The aim of the study was to assessment how EMR used as a heat source impacts the anaerobic processing of high-load poultry slaughterhouse wastewater (H-LPSW) and its performance. Microwave heating (MWH) was found to boost the CH4 fraction in the biogas under mesophilic conditions (35 °C) as long as the organic load rate (OLR) was maintained within 1.0 kgCOD/dm3·d to 4.0 kgCOD/dm3·d. The best performing variant—EPM heating (55 °C), OLR = 3.0 kgCOD/dm3·d, HRT = 5 days—produced 70.4 ± 2.7% CH4. High COD and TOC removal, as well as the highest biogas yields, were achieved for loadings of 1.0 gCOD/dm3·d to 4.0 gCOD/dm3·d. Effluent from the EMR-heated reactors (1.0 gCOD/dm3·d) contained, on average, 0.30 ± 0.07 gO2/dm3 at 55 °C and 0.38 ± 0.10 gO2/dm3 at 35 °C. The corresponding COD removal rates were 97.8 ± 0.6% and 98.1 ± 0.4%, respectively. The 5.0 gCOD/dm3·d and 6.0 gCOD/dm3·d OLR variants showed incremental decreases in performance. Based on the polymerase chain reaction results of 16S rDNA analysis, diversity of bacterial communities were mostly determined by OLR, not way of heating.
Theoretical and Experimental Analysis of the Thermal Response in Induction Thermography in the Frequency Range of 2.5 Hz to 20 kHz
Authors: Netzelmann Udo
Published: March 2023

The one-dimensional propagation of electromagnetic waves and the propagation of the resulting thermal waves in conducting material are analysed in a coherent way. The heat release due to resistive losses has a static and an oscillating part. Both are considered as heat source terms for the thermal diffusion equation. The time dependence of the temperature is described by analytical solutions. Electrically and thermally conducting materials are classified by the ratio of thermal penetration depth to the skin depth. Experiments performed on ferritic steel, stainless steel and carbon-fibre-reinforced polymer show the time dependence of the thermal signal after heating begins, as described by the theory. At low induction frequencies, an oscillating part of the surface temperature at the double of the induction frequency is detected in accordance with the theory. The results point out new opportunities for induction thermography.
Experiment and Simulation of the Startup Processes for the Supercritical Carbon-Dioxide Closed Brayton Cycle
Authors: Qin Shuo , Liang Shiqiang , Zhu Yuming , Li Zhigang , Gong Xinyu , Jiang Jiawei , Shen Zhixuan
Published: March 2023

The startup process is the crucial transition phase of the supercritical carbon-dioxide Brayton cycle, so it is essential to focus on and investigate the transient performance for the system’s safety and stability. The pressure in the buffer tank approaches the safety upper limit with different startup schemes during the joint commissioning of the compressor and heater in a MWe-scale experiment system, while the maximum temperature is 309 °C. Hence, dynamic simulations are carried out to explore the dynamic startup characteristics from a cold state or a warm state to the turbine pre-start condition, in which 60% of the rated mass flow rate and 67% of the rated compressor speed are reached in the end. The results show that, when starting from a cold state, the startup scheme of simultaneously heating and speeding up has a limited effective application scope. Two venting operations during the above process help the system establish heat regeneration and promote temperature uniformity in the system. Furthermore, when starting from a warm state with an existing temperature gradient in the system, the startup scheme of simultaneously heating and speeding up is more effective and has a more extensive range of control.
A Taxonomy of Idea Management Tools for Supporting Front-End Innovation
Authors: Zhu Di , Al Mahmud Abdullah , Liu Wei
Published: March 2023

Idea management is a crucial pillar of corporate management. Organizations may save research expenses, influence future development, and maintain distinctive competency by controlling front-end ideas. To date, several idea management tools have been developed. However, it is unknown to what extent they support the idea management process. Therefore, this scoping review aims to understand the classification of idea management tools and their effectiveness through an overview of the academic literature. Electronic databases (Scopus, ACM Digital Library, Web of Science Core Index, Elsevier ScienceDirect, and SpringerLink) were searched, and a total of 38 journal papers (n = 38) from 2010 to 2020 were retrieved. We identified 30 different types of idea management tools categorized as digital tools (n = 21), guidelines (n = 5), and frameworks (n = 4), and these tools have been utilized by software designers, hardware designers, and stakeholders. The identified tools may support various stages of idea management, such as capturing, generating, implementing, monitoring, refinement, retrieving, selection, and sharing. However, most tools only support a single stage (either capture or generate), and they cannot track the life cycle of the ideas, which may lead to misunderstanding. Therefore, it is essential to develop tools for managing ideas that would allow end users, designers, and other stakeholders to minimize bias in selecting and prioritizing ideas.
Properties and Fractal Analysis of High-Protein Milk Powders
Authors: Dec Bogdan , Kiełczewska Katarzyna , Smoczyński Michał , Baranowska Maria , Kowalik Jarosław
Published: March 2023

(1) Background: Optimization of production and evaluation of the quality of preparations containing milk proteins facilitates their use in various branches of the food industry. The aim of this study was to produce and characterize high-protein milk powders (MCC, SPC) obtained by membrane techniques, and to compare them with industrially produced powders (WPC, CH, WPH, WPI). (2) Methods: The composition, reconstitution and flow properties, particle size, and microstructure of milk powders were determined, and fractal analysis was performed. (3) Results: MCC and SPC produced by the membrane separation of skim milk and industrially produced powders were characterized by a wettability of >180 s and a high solubility (0.10–0.85 cm3), excluding MCC (10.75 cm3 of insoluble particles). Flowability expressed as the Carr index was very good in CH (<15%), good in MCC (15–20%), and fairly good in the remaining powders (20–25%). All powders were moderately cohesive, excluding CH, which was characterized by low cohesiveness. The analyzed preparations differed in the values of fractal dimension. (4) Conclusions: The reconstitution and rheological properties of high-protein milk powders were affected by their composition, particle size, porosity, and surface properties. The fractal approach to the microstructure of milk particles enabled the unambiguous detection of subtle differences in the microstructure of the analyzed samples, which could not be identified during a visual assessment.
Optimization of Laser-Assisted Polypropylene Aluminum Joining
Authors: Amne Elahi Mahdi , Marozzi Anthony , Plapper Peter
Published: March 2023

Laser joining of polymers to metals is a rising research subject due to the potential of considerably reducing the weight of structures. This article deals with the laser joining process between polypropylene and aluminum. Without pre-treatment, laser joining of these materials is not feasible, and the method applied in this study to circumvent this issue is a surface modification of aluminum with a pulsed laser to create mechanical interlocking for the heat conduction laser joining technique. Different patterns and various laser parameters are analyzed with the design of experiments to best understand the effects of each parameter along with microscopic observations. It is found that engraving weakens the mechanical properties of the aluminum samples. The compromise between the engraving depth and the mechanical properties of the samples is optimized, and the engraving process with a 0.28 mm line width, 27.3% density and 150 mm/s speed provides the highest mechanical performance of the assembly with minimum degradation of aluminum samples. Moreover, by adjusting the laser power and using power modulation below 300 W, the decomposition of polypropylene occurring at high temperatures is reduced to a minimum. After the final optimization, the joined samples reliably withstand a maximum force of 1500 N, which is, approximately, a shear strength of 20 MPa.
Effect of Heat Treatment on the Microstructure and Mechanical Properties of Rotary Friction Welded Dissimilar IN718 to SS304L Alloys
Authors: Pavan Perumandla , Talari Mahesh Kumar , Babu Nagumothu Kishore , Rehman Ateekh Ur , Srirangam Prakash
Published: March 2023

The present study investigated the effect of heat treatment (pre- and post-weld) on the microstructure and mechanical properties of an SS304L/IN718 dissimilar rotary friction welded alloy. Optical and scanning electron micrographs of the dissimilar rotary friction welded SS304L/IN718 joints in solution-treated (ST), solution-treated and aged (STA), and post-weld heat treatment (PWHT) conditions revealed defect-free welds. Furthermore, various zones were observed across the weld region, namely the fully deformed zone (FDZ), thermomechanical affected zone (TMAZ), heat affected zone (HAZ), and base material (BM). Among the SS304L/IN718 dissimilar friction welds with different heat treatment conditions (prior ST and STA, PWHT), the PWHTed dissimilar welds exhibited excellent mechanical properties, which could be attributed to the formation of the strengthening precipitates γ′ and γ″ during double aging in PWHT. In contrast, the mechanical properties were found to be the poorest in the STA condition, possibly due to the dissolution of the strengthening precipitates γ′ and γ″ during friction welding. It was observed that the SS304L/IN718 dissimilar friction welds in the ST and STA conditions failed in the HAZ of the SS304L side, away from the weld interface, indicating that the weld region was stronger than the weakest base metal (SS304L) in the various joints.