bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing Placebos for Clinical Research in Traditional Chinese Medicine: Assessing Organoleptic Properties of Three Dosage Forms (Oral Liquid, Capsule and Granule)
Authors: Xiao Mengli , Ying Jiake , Zhao Yang , Li Qingna , Zhao Yingpan , Gao Rui , Lu Fang
Published: June 2021

Background: The successful application of randomized, double-blind placebo-controlled studies requires maximum blinding. Organoleptic properties of the placebo should be similar to the drug, making it difficult to distinguish between the two. The uniqueness of traditional Chinese medicine (TCM) preparations makes it challenging to prepare placebo. Evaluation of the TCM placebo simulation effect can determine whether the preparation of placebo can be genuinely blind in clinical trials. There is still a lack of well-established methods to evaluate TCM placebos. Hence, this study aimed to explore the evaluation methodology of TCM placebo simulation.

Methods: An independent evaluation method and three comparative evaluation methods were proposed, and three dosage forms (oral liquid, capsule, and granule) were tested. The independent evaluation, in which each person was given an experimental drug or a placebo, gave an overall assessment of organoleptic properties in a blind state. We comparatively evaluated the similarity in organoleptic properties between the experimental drug and placebo. According to different distribution methods, we divided comparative evaluation methods into three. In method 1, the evaluator was given the experimental drug and placebo and was told that there must be a placebo among them. In method 2, each evaluator was randomly assigned to the combination group or two investigational drugs group. In method 3, the evaluator was assigned to a set of three coded samples, numbered by random three-digit numbers, each different, two of which were identical, and the two samples were equally frequent.

Results: In the independent evaluation, there was no difference between TCM placebo and experimental drugs in a blind state at the level of p = 0.05. Even though the comparative evaluation methods enabled identification of potential differences between the two samples, methods 2 and 3 were better than method 1 in eliminating psychological factors. Also, in method 3, the completely random method combined with the blind method eliminated the subjectivity and objectivity bias and improved the experiment’s credibility compared with the previous two methods.

Conclusion: Regardless of the methods that could evaluate the placebo’s simulated effect in actual clinical trials, we suggest that independent evaluation and comparative evaluation (method 3) should be combined to reflect better whether the placebo is truly blind.
A Randomized, Double-Blind, Single-Dose Study Comparing the Biosimilarity of HOT-1010 With Bevacizumab (Avastin®) in Chinese Healthy Male Subjects
Authors: Huang Kai , Que Linling , Ding Ying , Chu Nannan , Qian Zhenzhong , Shi Yunfei , Qin Wei , Li Zhenni , Chen Yuanxin , Gu Xianghong , Wang Jiakun , Zhang Lin , Zhang Jisheng , Zhu Xiangyang , Yang Yongmin , Tang Yuan , He Qing
Published: June 2021

Objective: This study was conducted to compare the pharmacokinetics, safety and immunogenicity of HOT-1010 with bevacizumab (Avastin®) in Chinese healthy male subjects.

Methods: A single-center, randomized, double-blind, single-dose, parallel trial was performed in 84 Chinese healthy male subjects who randomly (1:1) received a single intravenous infusion of 1 mg/kg HOT-1010 or Avastin® for 90 min and followed up for 85 days. Serum concentrations of bevacizumab were analyzed by enzyme-linked immunosorbent assay. Primary pharmacokinetic parameters, Cmax, AUC0-t and AUC0-∞, were calculated and evaluated the bioequivalence between HOT-1010 and Avastin®, the safety and immunogenicity of investigational drugs were also assessed.

Results: A total of 82 subjects completed the study. The 90% Confidence Intervals for geometric mean ratios of Cmax, AUC0-t and AUC0-∞ were 91.81–103.64%, 85.19–95.39% and 85.04–95.36%, which were all within the bioequivalence margin. Treatment-emergent adverse events were reported in 27 (65.9%) subjects in HOT-1010 group and 23 (56.1%) subjects in Avastin® group. Most TEAEs were mild or moderate. No TEAEs, Serious Adverse Events or deaths leading to discontinuation was reported. Subjects were all tested negative for Anti-drug Antibody.

Conclusion: HOT-1010 exhibited the similar pharmacokinetics, safety and immunogenicity profiles of bevacizumab (Avastin®) in Chinese healthy male subjects.

Clinical Trial Registration:http://www.chinadrugtrials.org.cn/index.html, CTR20181610.
Effect of Cigarette Smoke on Gut Microbiota: State of Knowledge
Authors: Gui Xiaohua , Yang Zhongli , Li Ming D.
Published: June 2021

Cigarette smoke is a representative source of toxic chemical exposures to humans, and the adverse consequences of cigarette smoking are mediated by its effect on both neuronal and immune–inflammatory systems. Cigarette smoking also is a major risk factor for intestinal disorders, such as Crohn’s disease and peptic ulcer. On the other hand, cigarette smoking is protective against developing ulcerative colitis. The effects of cigarette smoking on intestinal disorders include changes in intestinal irrigation and microbiome, increases in permeability of the mucosa, and impaired mucosal immune responses. However, the underlying mechanism linking cigarette smoking with intestinal microbiota dysbiosis is largely unknown. In this communication, we first review the current knowledge about the mechanistic interaction between cigarette smoke and intestinal microbiota dysbiosis, which include the likely actions of nicotine, aldehydes, polycyclic aromatic hydrocarbons, heavy metals, volatile organic compounds and toxic gases, and then reveal the potential mechanisms of the lung–gut cross talk and skin-gut cross talk in regulating the balance of intestinal microbiota and the interrelation of intestinal microbiota dysbiosis and systemic disorders.
A Systematic Review and Meta-Analysis Examining Whether Changing Ovarian Sex Steroid Hormone Levels Influence Cerebrovascular Function
Authors: Skinner Bethany D. , Davies Rebecca J. , Weaver Samuel R. , Cable N. Tim , Lucas Samuel J. E. , Lucas Rebekah A. I.
Published: June 2021

Sex differences in cerebrovascular disease rates indicate a possible role for ovarian sex steroid hormones in cerebrovascular function. To synthesise and identify knowledge gaps, a systematic review and meta-analysis was conducted to assess how ovarian sex steroid hormone changes across the lifespan affect cerebrovascular function in women. Three databases (EMBASE, MEDLINE and Web of Science) were systematically searched for studies on adult cerebrovascular function and ovarian sex steroid hormones. Forty-five studies met pre-defined inclusion criteria. Studied hormone groups included hormone replacement therapy (HRT; n = 17), pregnancy (n = 12), menstrual cycle (n = 7), menopause (n = 5), oral contraception (n = 2), and ovarian hyperstimulation (n = 2). Outcome measures included pulsatility index (PI), cerebral blood flow/velocity (CBF), resistance index (RI), cerebral autoregulation, and cerebrovascular reactivity. Meta-analysis was carried out on HRT studies. PI significantly decreased [−0.05, 95% CI: (−0.10, −0.01); p = 0.01] in post-menopausal women undergoing HRT compared to post-menopausal women who were not, though there was considerable heterogeneity (I2 = 96.8%). No effects of HRT were seen in CBF (p = 0.24) or RI (p = 0.77). This review indicates that HRT improves PI in post-menopausal women. However, there remains insufficient evidence to determine how changing ovarian sex steroid hormone levels affects cerebrovascular function in women during other hormonal phases (e.g., pregnancy, oral contraception).
Phenotyping Flowering in Canola (Brassica napus L.) and Estimating Seed Yield Using an Unmanned Aerial Vehicle-Based Imagery
Authors: Zhang Ti , Vail Sally , Duddu Hema S. N. , Parkin Isobel A. P. , Guo Xulin , Johnson Eric N. , Shirtliffe Steven J.
Published: June 2021

Phenotyping crop performance is critical for line selection and variety development in plant breeding. Canola (Brassica napus L.) flowers, the bright yellow flowers, indeterminately increase over a protracted period. Flower production of canola plays an important role in yield determination. Yellowness of canola petals may be a critical reflectance signal and a good predictor of pod number and, therefore, seed yield. However, quantifying flowering based on traditional visual scales is subjective, time-consuming, and labor-consuming. Recent developments in phenotyping technologies using Unmanned Aerial Vehicles (UAVs) make it possible to effectively capture crop information and to predict crop yield via imagery. Our objectives were to investigate the application of vegetation indices in estimating canola flower numbers and to develop a descriptive model of canola seed yield. Fifty-six diverse Brassica genotypes, including 53 B. napus lines, two Brassica carinata lines, and a Brassica juncea variety, were grown near Saskatoon, SK, Canada from 2016 to 2018 and near Melfort and Scott, SK, Canada in 2017. Aerial imagery with geometric and radiometric corrections was collected through the flowering stage using a UAV mounted with a multispectral camera. We found that the normalized difference yellowness index (NDYI) was a useful vegetation index for representing canola yellowness, which is related to canola flowering intensity during the full flowering stage. However, the flowering pixel number estimated by the thresholding method improved the ability of NDYI to detect yellow flowers with coefficient of determination (R2) ranging from 0.54 to 0.95. Moreover, compared with using a single image date, the NDYI-based flowering pixel numbers integrated over time covers more growth information and can be a good predictor of pod number and thus, canola yield with R2 up to 0.42. These results indicate that NDYI-based flowering pixel numbers can perform well in estimating flowering intensity. Integrated flowering intensity extracted from imagery over time can be a potential phenotype associated with canola seed yield.
An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives
Authors: Kaur Amarpreet , Batish Daizy R. , Kaur Shalinder , Chauhan Bhagirath S.
Published: June 2021

Calotropis procera(Aiton) Dryand. (commonly known as the apple of sodom, calotrope, and giant milkweed) is an evergreen, perennial shrub of the family Apocynaceae, mainly found in arid and semi-arid regions. It is a multipurpose plant, which can be utilized for medicine, fodder, and fuel purposes, timber and fiber production, phytoremediation, and synthesis of nanoparticles. It has been widely used in traditional medicinal systems across North Africa, Middle East Asia, and South-East Asia. At present, it is being extensively explored for its potential pharmacological applications. Several reports also suggest its prospects in the food, textile, and paper industries. Besides,C.procerahas also been acknowledged as an ornamental species. High pharmacological potential and socio-economic value have led to the pantropical introduction of the plant. Morpho-physiological adaptations and the ability to tolerate various abiotic stresses enabled its naturalization beyond the introduced areas. Now, it is recognized as an obnoxious environmental weed in several parts of the world. Its unnatural expansion has been witnessed in the regions of South America, the Caribbean Islands, Australia, the Hawaiian Islands, Mexico, Seychelles, and several Pacific Islands. In Australia, nearly 3.7 million hectares of drier areas, including rangelands and Savannahs, have been invaded by the plant. In this review, multiple aspects ofC.procerahave been discussed including its general characteristics, current and potential uses, and invasive tendencies. The objectives of this review are a) to compile the information available in the literature onC.procera, to make it accessible for future research, b) to enlist together its potential applications being investigated in different fields, and c) to acknowledgeC.proceraas an emerging invasive species of arid and semi-arid regions.
Diversity of Plastid Types and Their Interconversions
Authors: Choi Heebak , Yi Taegyu , Ha Sun-Hwa
Published: June 2021

Plastids are pivotal subcellular organelles that have evolved to perform specialized functions in plant cells, including photosynthesis and the production and storage of metabolites. They come in a variety of forms with different characteristics, enabling them to function in a diverse array of organ/tissue/cell-specific developmental processes and with a variety of environmental signals. Here, we have comprehensively reviewed the distinctive roles of plastids and their transition statuses, according to their features. Furthermore, the most recent understanding of their regulatory mechanisms is highlighted at both transcriptional and post-translational levels, with a focus on the greening and non-greening phenotypes.
Optogenetic Dissection of Neural Circuits Underlying Stress-Induced Mood Disorders
Authors: Liu Qing , Zhang Zhinuo , Zhang Wenjuan
Published: June 2021

Objectives: This review aims to (i) summarize the literature on optogenetic applications of different stress-induced mood disorder models of the medial prefrontal cortex (mPFC) and its projection circuits, and (ii) examine methodological variability across the literature and how such variations may influence the underlying circuits of stress-induced mood disorders.

Methods: A variety of databases (PubMed, Web of Science, Elsevier, Springer, and Wiley) were systematically searched to identify optogenetic studies that applied to mood disorders in the context of stress.

Results: Eleven studies on optogenetic stimulation of the mPFC and the effect of its efferent circuitry on anxiety- and depression-like behaviors in different rodent models were selected. The results showed that the optogenetics (i) can provide insights into the underlying circuits of mood disorders in the context of stress (ii) and also points out new therapeutic strategies for treating mood disorders.

Conclusions: These findings indicate a clear role for the mPFC in social avoidance, and highlight the central role of stress reactivity circuitry that may be targeted for the treatment of stress-induced mood disorders.
A Combined Effect of Self and Reward: Relationship of Self- and Reward-Bias on Associative Learning
Authors: Wang Lingyun , Qi Yuxin , Li Lihong , Jia Fanli
Published: June 2021

Previous studies have demonstrated that individuals process information related to themselves or a high reward quickly and have referred to this as self-bias or reward-bias. However, no previous study has presented self- and reward-bias simultaneously. The present study investigated perceptual processing using the associated learning paradigm when both self and reward were prioritized (condition of double salience) as well as when only self or reward was prioritized (condition of single salience). The present study established these two conditions by manipulating self-relevance (self vs. stranger in Experiment 1; self vs. friend in Experiment 2). The results showed that (1) when the self was pitted against a stranger and received a high or low reward, perceptual processing of the participants mainly involved self-bias (Experiment 1); (2) when the self was pitted against a friend, perceptual processing involved both self-bias and reward-bias (Experiment 2). The study revealed a complex relationship between self- and reward-bias, which depends on the degree of affinity between oneself and others.
Tailoring the Input to Children's Needs: The Use of Fine Lexical Tuning in Speech Directed to Normally Hearing Children and Children With Cochlear Implants
Authors: Odijk Lotte , Gillis Steven
Published: June 2021

Purpose: The aim of the present study was to explore fine lexical tuning in Dutch infant-directed speech (IDS) addressed to congenitally deaf infants who received a cochlear implant (CI) early in life (<2 years of age) in comparison with children with normal hearing (NH). The longitudinal pattern of parents' utterance length in the initial stages of the child's lexical development was examined. Parents' utterances containing the words the children eventually acquired in the earliest developmental stages were selected and their MLU (Mean Length of Utterance) was measured.

Method: Transcriptions of monthly recordings of spontaneous interactions of 10 CI children and 30 NH children with their parents were analyzed. The children with CI were followed from the moment their device was switched on, and the NH children from the age of 6 months onwards. A total of 57,846 utterances of parents of CI children and 149,468 utterances of parents of NH children were analyzed.

Results: IDS addressed to children with NH and children with CI exhibits fine lexical tuning: parents adjust the MLU of the utterances that contain the words that children are on the verge of producing themselves. More specifically, the parents' mean length of those utterances decreased in relation to the point when the children began using the item. Consequently, the number of occurrences in isolation of the lexical item increased. The speech addressed to all the children exhibited this phenomenon, but it was significantly more strongly present in speech addressed to the children with CI.

Conclusions: The speech addressed to children with NH and CI is characterized by fine lexical tuning and a high incidence of single-word utterances in the period leading up to the children's first use of words in speech production. Notwithstanding striking commonalities, IDS addressed to children with a hearing impairment is markedly different, which suggests that parents take this specific character of the children into account.
Revisiting the Age-Prospective Memory Paradox Using Laboratory and Ecological Tasks
Authors: Koo Yu Wen , Neumann David L. , Ownsworth Tamara , Shum David H. K.
Published: June 2021

Prospective memory (PM) is the ability to perform a planned action at a future time. Older adults have shown moderate declines in PM, which are thought to be driven by age-related changes in the prefrontal cortex. However, an age-PM paradox is often reported, whereby deficits are evident in laboratory-based PM tasks, but not naturalistic PM tasks. The key aims of this study were to: (1) examine the age-PM paradox using the same sample across laboratory and ecological settings; and (2) determine whether self-reported PM and cognitive factors such as working memory and IQ are associated PM performance. Two PM tasks were administered (ecological vs. laboratory) to a sample of 23 community-dwelling older adults (Mage= 72.30,SDage= 5.62) and 28 young adults (Mage= 20.18,SDage= 3.30). Participants also completed measures of general cognitive function, working memory, IQ, and self-reported memory. Our results did not support the existence of the age-PM paradox. Strong age effects across both laboratory and ecological PM tasks were observed in which older adults consistently performed worse on the PM tasks than young adults. In addition, PM performance was significantly associated with self-reported PM measures in young adults. For older adults, IQ was associated with time-based PM. These findings suggest that the age-PM paradox is more complex than first thought and there are differential predictors of PM performance for younger and older adults.
Perspectives on Recruitment and Representativeness in Forensic Psychiatric Research
Authors: Pedersen Sven H. , Bergman Henrik , Berlin Johan , Hartvigsson Thomas
Published: June 2021

Participant representativeness and statistical power are crucial elements of robust research with human participants, both of which relate to the successful recruitment of research participants. Nevertheless, such core features may often not be fully reported or duly considered in psychiatric research. Building on our experiences of collecting data in the context of forensic mental health services, we discuss issues regarding participant recruitment and representativeness in our field with its particular characteristics. A quick sampling and brief overview of the literature in four specialized forensic mental health journals is presented, demonstrating that published manuscripts rarely describe the data in sufficient detail for the reader to assess sample representativeness and statistical power. This lack of transparency leads not only to difficulties in interpreting the research; it also entails risks relating to the already meager evidence base of forensic mental health services being relevant only to a subset of patients. Accordingly, we provide suggestions for increased transparency in reporting and improved recruitment of research participants. We also discuss the balance of ethical considerations pertinent to the pursuit of increased participation rates in forensic mental health research.
Barriers and Enablers to Shared Decision Making in Psychiatric Medication Management: A Qualitative Investigation of Clinician and Service Users' Views
Authors: Kaminskiy Emma , Zisman-Ilani Yaara , Morant Nicola , Ramon Shulamit
Published: June 2021

Shared decisionmaking (SDM) is a recommended health communication approach in mental health settings. Yet, implementation of SDM in psychiatric consultations discussing medication management is challenging. Insufficient attention has been given to examine the views of both clinicians and service users together about the experiences of SDM in psychiatric medication management. The purpose of this paper is to examine the views of service users, community psychiatric nurses, and psychiatrists about enablers and barriers of SDM. A thematic analysis of 30 semi structured interviews with service users, psychiatrists, and community psychiatric nurses, in a community mental health team in the UK, was conducted. A service user advisory group was involved in all phases of the research cycle, including data collection, analysis, and dissemination. The results offer a detailed contextualized account of how medication decisions are made. For psychiatrists and service user participants SDM is seen as a way of enhancing service users' engagement in and control over treatment decisions. While psychiatrists value the transactional benefits of SDM, service user participants and psychiatric nurses conceptualize SDM as a long-term endeavor embedded within therapeutic partnerships. For service users these partnerships mitigate acknowledged problems of feeling unable to be fully involved during times of crisis. This study identified a range of barriers and facilitators to SDM concerning psychiatric medications from the lived experience of service users and the professional experience of clinicians. Furthermore, it indicates new potential intervention points to support SDM in psychiatric medication decisions.
Antimicrobial Resistance Profiles of Coagulase-Negative Staphylococci in Community-Based Healthy Individuals in Germany
Authors: Marincola Gabriella , Liong Olivia , Schoen Christoph , Abouelfetouh Alaa , Hamdy Aisha , Wencker Freya D. R. , Marciniak Tessa , Becker Karsten , Köck Robin , Ziebuhr Wilma
Published: June 2021

Coagulase-negative staphylococci (CoNS) are common opportunistic pathogens, but also ubiquitous human and animal commensals. Infection-associated CoNS from healthcare environments are typically characterized by pronounced antimicrobial resistance (AMR) including both methicillin- and multidrug-resistant isolates. Less is known about AMR patterns of CoNS colonizing the general population. Here we report on AMR in commensal CoNS recovered from 117 non-hospitalized volunteers in a region of Germany with a high livestock density. Among the 69 individuals colonized with CoNS, 29 had reported contacts to either companion or farm animals. CoNS were selectively cultivated from nasal swabs, followed by species definition by 16S rDNA sequencing and routine antibiotic susceptibility testing. Isolates displaying phenotypic AMR were further tested by PCR for presence of selected AMR genes. A total of 127 CoNS were isolated and Staphylococcus epidermidis (75%) was the most common CoNS species identified. Nine isolates (7%) were methicillin-resistant (MR) and carried the mecA gene, with seven individuals (10%) being colonized with at least one MR-CoNS isolate. While resistance against gentamicin, phenicols and spectinomycin was rare, high resistance rates were found against tetracycline (39%), erythromycin (33%) and fusidic acid (24%). In the majority of isolates, phenotypic resistance could be associated with corresponding AMR gene detection. Multidrug-resistance (MDR) was observed in 23% (29/127) of the isolates, with 33% (23/69) of the individuals being colonized with MDR-CoNS. The combined data suggest that MR- and MDR-CoNS are present in the community, with previous animal contact not significantly influencing the risk of becoming colonized with such isolates.
Science Diplomacy in Latin America and the Caribbean: Current Landscape, Challenges, and Future Perspectives
Authors: Soler Marga Gual
Published: June 2021

Science, technology, and innovation are taking center stage in international affairs and increasingly influencing the geopolitical dynamics and a country's standing on the global stage. New scientific and technological advancements are acquiring greater strategic relevance to ensure competitive advantages in the twenty-first century global order. At the same time, international scientific collaboration contributes to generating and democratizing knowledge and improving relations between countries as a “soft power” tool to coordinate science-based solutions to transboundary problems, and to build bridges between countries with tense diplomatic relations. Science diplomacy is not a new concept, but most of its intellectual foundations and practical applications have emerged in the Global North. This article describes the diverse approaches, policies and practices adopted by Latin American and Caribbean countries at the national, sub-national, and regional levels. It analyzes their successes and challenges and identifies opportunities to guide the region toward a common science diplomacy strategy to achieve sustainable development through incorporating science as a permanent element in the foreign policy toolkit of Latin American nations. By documenting and illuminating best practices in the region, this article also seeks to balance the emphasis that has so far been largely concentrated on the regions of Europe and North America and contribute to future efforts and strategies for the development of sustainable science diplomacy mechanisms at the national, regional, North-South and South-South levels.
A Minimal Design of a Human Infant Presence: A Case Study Toward Interactive Doll Therapy for Older Adults With Dementia
Authors: Sumioka Hidenobu , Yamato Nobuo , Shiomi Masahiro , Ishiguro Hiroshi
Published: June 2021

We introduce a minimal design approach to manufacture an infant-like robot for interactive doll therapy that provides emotional interactions for older people with dementia. Our approach stimulates their imaginations and then facilitates positive engagement with the robot by just expressing the most basic elements of humanlike features. Based on this approach, we developed HIRO, a baby-sized robot with an abstract body representation and no facial features. The recorded voice of a real human infant emitted by robots enhances the robot’s human-likeness and facilitates positive interaction between older adults and the robot. Although we did not find any significant difference between HIRO and an infant-like robot with a smiling face, a field study showed that HIRO was accepted by older adults with dementia and facilitated positive interaction by stimulating their imagination. We also discuss the importance of a minimal design approach in elderly care during post–COVID-19 world.
Criticality-Driven Evolution of Adaptable Morphologies of Voxel-Based Soft-Robots
Authors: Talamini Jacopo , Medvet Eric , Nichele Stefano
Published: June 2021

The paradigm of voxel-based soft robots has allowed to shift the complexity from the control algorithm to the robot morphology itself. The bodies of voxel-based soft robots are extremely versatile and more adaptable than the one of traditional robots, since they consist of many simple components that can be freely assembled. Nonetheless, it is still not clear which are the factors responsible for the adaptability of the morphology, which we define as the ability to cope with tasks requiring different skills. In this work, we propose a task-agnostic approach for automatically designing adaptable soft robotic morphologies in simulation, based on the concept of criticality. Criticality is a property belonging to dynamical systems close to a phase transition between the ordered and the chaotic regime. Our hypotheses are that 1) morphologies can be optimized for exhibiting critical dynamics and 2) robots with those morphologies are not worse, on a set of different tasks, than robots with handcrafted morphologies. We introduce a measure of criticality in the context of voxel-based soft robots which is based on the concept of avalanche analysis, often used to assess criticality in biological and artificial neural networks. We let the robot morphologies evolve toward criticality by measuring how close is their avalanche distribution to a power law distribution. We then validate the impact of this approach on the actual adaptability by measuring the resulting robots performance on three different tasks designed to require different skills. The validation results confirm that criticality is indeed a good indicator for the adaptability of a soft robotic morphology, and therefore a promising approach for guiding the design of more adaptive voxel-based soft robots.
Viruses—Agents of Change in the Oceans
Authors: Avrani Sarit , Sher Daniel
Published: June 2021

Viruses are usually thought of as the cause of countless diseases. However, in the oceans, viruses are part of the natural cycle of life and death. This article discusses marine viruses that infect phytoplankton—the tiny micro-algae that form the base of the marine food web and affect Earth’s climate. Through an ongoing “arms race” between viruses and the cells they infect, viruses can promote the evolution of their hosts, and even help their hosts acquire genes that can help them survive. By killing phytoplankton species that become very abundant, viruses can allow other species to grow, promoting biodiversity. Finally, viruses affect global cycles of carbon and other elements, indirectly influencing the climate of our planet.
Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows
Authors: Lima Ewerton de Souza , Blagitz Maiara Garcia , Batista Camila Freitas , Alves Alexandre José , Fernandes Artur Cezar de Carvalho , Ramos Sanchez Eduardo Milton , Frias Torres Hugo , Diniz Soraia Araújo , Silva Marcos Xavier , Della Libera Alice Maria Melville Paiva , de Souza Fernando Nogueira
Published: June 2021

The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production of reactive oxygen and nitrogen species (RONS) by macrophages in milk samples from dairy cows naturally infected with BLV with or without persistent lymphocytosis (PL). No effect of BLV infection in the overall number of macrophages per milliliter and in the percentage of viable macrophages among overall milk viable cells was found. Furthermore, BLV-infected dairy cows had a higher frequency of viable milk macrophages, while healthy animals had a tendency toward a higher percentage of apoptotic milk macrophages. The percentage of milk macrophages that phagocytosedStaphylococcus aureusin seronegative animals was higher than that in BLV-infected dairy cows. No effect of BLV infection on the intracellular RONS production and the intensity of phagocytosis by milk macrophages was observed. Thus, this study provides new insights into the implications of BLV infections in the bovine mammary gland.
Perception of Person-Centred Maternity Care and Its Associated Factors Among Post-Partum Women: Evidence From a Cross-Sectional Study in Enugu State, Nigeria
Authors: Ogbuabor Daniel C. , Nwankwor Chikezie
Published: June 2021

Objectives: This study validated a person-centred maternity care (PCMC) scale and assessed perception of PCMC and its associated factors among post-partum women.

Methods: A cross-sectional study was conducted among 450 post-partum women in two districts in Enugu State, Nigeria, using a 30-item PCMC scale. Exploratory and confirmatory factor analyses, descriptive, bivariate and Generalized Linear Models analyses were conducted.

Results: Twenty-two items were retained in the PCMC scale with high internal reliability and goodness-of-fit indices. About 25% of women received high PCMC. Marrying at 20–29 years (β = 3.46, ρ = 0.017) and 30–49 years (β = −5.56, ρ = 0.020); self-employment (β = −7.50, ρ = 0.005); marrying government worker (β = 7.12, ρ = 0.020); starting antenatal care in the third trimester (β = −6.78, ρ = 0.003); high participation in decision-making (β = −10.41, ρ < 0.001); domestic violence experience (β = 3.60, ρ = 0.007); delivery at health centre (β = 18.10, ρ < 0.001), private/mission hospital (β = 4.01, ρ = 0.003), by non-skilled attendant (β = −16.55, ρ < 0.001) and community health worker (β = −10.30, ρ < 0.001); and pregnancy complication (β = 4.37, ρ = 0.043) influenced PCMC.

Conclusion: The PCMC scale is valid and reliable in Nigeria. PCMC requires improvement in Enugu State. This study identified factors that may be considered for inclusion in intervention strategies.
An Overview of the Epidemiologic, Diagnostic and Treatment Approaches of COVID-19: What do We Know?
Authors: Beyrampour-Basmenj Hanieh , Milani Morteza , Ebrahimi-Kalan Abbas , Ben Taleb Ziyad , Ward Kenneth D , Dargahi Abbasabad Ghader , Aliyari-serej Zeynab , Ebrahimi Kalan Mohammad
Published: June 2021

Background:In late December 2019, a new infectious respiratory disease (COVID-19) was reported in a number of patients with a history of exposure to the Huanan seafood market in China. The World Health Organization officially announced the COVID-19 pandemic on March 11, 2020. Here, we provided an overview of the epidemiologic, diagnostic and treatment approaches associated with COVID-19.

Methods:We reviewed the publications indexed in major biomedical databases by December 20, 2020 or earlier (updated on May 16, 2021). Search keywords included a combination of: COVID-19, Coronavirus disease 2019, SARS-CoV-2, Epidemiology, Prevention, Diagnosis, Vaccine, and Treatment. We also used available information about COVID-19 from valid sources such as WHO.

Results and Conclusion:At the time of writing this review, while most of the countries authorized COVID-19 vaccines for emergency use starting December 8, 2020, there is no a definite cure for it. This review synthesizes current knowledge of virology, epidemiology, clinical symptoms, diagnostic approaches, common treatment strategies, novel potential therapeutic options for control and prevention of COVID-19 infection, available vaccines, public health and clinical implications.
Authentic Talent Development in Women Leaders Who Opted Out: Discovering Authenticity, Balance, and Challenge through the Kaleidoscope Career Model
Authors: Knowles Jennifer , Mainiero Lisa
Published: June 2021

We investigated the parameters of authenticity, balance, and challenge as viewed through the lens of the Kaleidoscope Career Model to discern the career motives of women who opted out and then returned to the workforce. We also were interested in comparing women and men in their leader practices. We triangulated quantitative and qualitative methods to explore these phenomena. First, in Study 1, 2009 individuals completed both the Kaleidoscope Career Self Inventory (the KCSI) as well as an abbreviated version of Kouzes and Posner’s Leadership Practices Inventory (the LPI). Participants rated their needs for authenticity, balance, and challenge on the KCSI as well as their leader practices of challenging the process, inspiring a shared vision, modeling the way, enabling others to act, and Encouraging the Heart. Women were found to exceed the leader practices of men, and women were most interested in authenticity. In Study 2, situational mapping and life history process was used to determine themes of eight high-achieving women who opted out. We interviewed an additional 15 women to deeply understand and assess their opt-out and re-entry experiences as well as “career shocks” they experienced upon returning to the workforce. Our second study offers a robust, deep, penetrating look into social ascription processes and endemic discriminatory social structures that hold women back from achieving advancement. To stop this “brain drain” of talent, we propose a series of actions for human resource professionals to develop the authentic leadership talent of women who reenter the workforce.
Genotyping of Two Mediterranean Trout Populations in Central-Southern Italy for Conservation Purposes Using a Rainbow-Trout-Derived SNP Array
Authors: Palombo Valentino , De Zio Elena , Salvatore Giovanna , Esposito Stefano , Iaffaldano Nicolaia , D’Andrea Mariasilvia
Published: June 2021

Mediterranean trout is a freshwater fish of particular interest with economic significance for fishery management, aquaculture and conservation biology. Unfortunately, native trout populations’ abundance is significantly threatened by anthropogenic disturbance. The introduction of commercial hatchery strains for recreation activities has compromised the genetic integrity status of native populations. This work assessed the fine-scale genetic structure of Mediterranean trout in the two main rivers of Molise region (Italy) to support conservation actions. In total, 288 specimens were caught in 28 different sites (14 per basins) and genotyped using the Affymetrix 57 K rainbow-trout-derived SNP array. Population differentiation was analyzed using pairwise weighted FST and overall F-statistic estimated by locus-by-locus analysis of molecular variance. Furthermore, an SNP data set was processed through principal coordinates analysis, discriminant analysis of principal components and admixture Bayesian clustering analysis. Firstly, our results demonstrated that rainbow trout SNP array can be successfully used for Mediterranean trout genotyping. In fact, despite an overwhelming number of loci that resulted as monomorphic in our populations, it must be emphasized that the resulted number of polymorphic loci (i.e., ~900 SNPs) has been sufficient to reveal a fine-scale genetic structure in the investigated populations, which is useful in supporting conservation and management actions. In particular, our findings allowed us to select candidate sites for the collection of adults, needed for the production of genetically pure juvenile trout, and sites to carry out the eradication of alien trout and successive re-introduction of native trout.
Long-Term Determinants of the Seroprevalence of the Hepatitis E Virus in Wild Boar (Sus scrofa)
Authors: Barroso Patricia , Risalde María A. , García-Bocanegra Ignacio , Acevedo Pelayo , Barasona José Ángel , Caballero-Gómez Javier , Jiménez-Ruiz Saúl , Rivero-Juárez Antonio , Montoro Vidal , Vicente Joaquín
Published: June 2021

The hepatitis E virus (HEV) is an emerging zoonotic pathogen whose main reservoir is suids. Most of the ecological and epidemiological aspects of its sylvatic cycle remain unknown. Thus, in this work, we study the drivers of HEV exposure in the wild boar population of Doñana National Park (DNP, southwest Spain) operating in the medium and long-term (2005–2018). Anti-HEV antibodies are widely distributed throughout the wild boar (46.7 ± 3.8%, 327 out of 700 sampled), showing a statistically significant age-increasing pattern. The temporal pattern displayed important interannual fluctuations. This could be mediated by marked variations in the population control of the wild boar, and subsequent changes in abundance rates, and its interplay with climatic conditions; as wet years together with a low abundance of wild boar led to the lowest seroprevalence. The fact that seroprevalence is high during conditions of high abundance, and not affected by rainfall level, is probably due to the increased interactions among the animals, and possibly, the subsequent higher environmental contamination with HEV particles. The proximity to the marshland (the main water body of the study area) is associated with a higher risk of testing positive, which is probably mediated by the preferential use of this area during the dry season and the favourable environmental conditions for the survival of HEV particles. A deeper understanding of the epidemiology of HEV in host communities deserves future research concerning other susceptible species. Most importantly, wild boar population control remains a challenge at the international level, and an increase of shared pathogen-related conflicts associated with this species is expected, as exemplified by HEV. Therefore, surveillance of wild boar diseases, including integrated population monitoring and sustainable population control programmes, will be essential to control the associated risks.
Research Trends in Octopus Biological Studies
Authors: Di Cosmo Anna , Pinelli Claudia , Scandurra Anna , Aria Massimo , D’Aniello Biagio
Published: June 2021

Octopuses represent interesting model studies for different fields of scientific inquiry. The present study provides a bibliometric analysis on research trends in octopuses biological studies. The analysis was executed from January 1985 to December 2020 including scientific products reported in the Web of Science database. The period of study was split into two blocks (“earlier period” (EP): 1985−2010; “recent period” (RP): 2011−2020) to analyze the evolution of the research topics over time. All publications of interest were identified by using the following query: ((AK = octopus) OR (AB = octopus) OR (TI = octopus)). Data information was converted into an R-data frame using bibliometrix. Octopuses studies appeared in 360 different sources in EP, while they increased to 408 in RP. Sixty countries contributed to the octopuses studies in the EP, while they were 78 in the RP. The number of affiliations also increased between EP and RP, with 835 research centers involved in the EP and 1399 in the RP. In the EP 5 clusters (i.e., “growth and nutrition”, “pollution impact”, “morphology”, “neurobiology”, “biochemistry”) were represented in a thematic map, according to their centrality and density ranking. In the RP the analysis identified 4 clusters (i.e., “growth and nutrition”, “ecology”, “pollution impact”, “genes, behavior, and brain evolution”). The UK with Ireland, and the USA with Canada shared the highest number of publications in the EP, while in the RP, Spain and Portugal were the leading countries. The current data provide significant insight into the evolving trends in octopuses studies.
Multi-Approach Assessment for Stress Evaluation in Rainbow Trout Females, Oncorhynchus mykiss (Walbaum, 1792) from Three Different Farms during the Summer Season
Authors: Uiuiu Paul , Lațiu Călin , Păpuc Tudor , Craioveanu Cristina , Ihuț Andrada , Sava Alexandru , Răducu Camelia , Șonea Cosmin , Constantinescu Radu , Cocan Daniel , Mireșan Vioara
Published: June 2021

Blood biochemistry parameters are valuable tools for monitoring fish health. Their baseline values are still undefined for a multitude of farmed fish species. In this study, changes in the blood profile of rainbow trout females (Oncorhynchus mykiss) from three farms were investigated using different biomarkers during the summer season. In the given context, the main water physicochemical parameters were investigated and twelve biochemical parameters were measured from blood samples of rainbow trout reared in the Fiad, Șoimul de Jos, and Strâmba farms. We selected these farms because the genetic background of the rainbow trout is the same, with all studied specimens coming from the Fiad farm, which has an incubation station. Forty-five samples were collected monthly (May to August) throughout summer to observe the changes in the blood profile of rainbow trout. Principal component analysis showed a clear separation both among the studied farms and months. Furthermore, significant correlations (p < 0.05) between the majority of the biochemical parameters were found, indicating that the environmental parameters can influence several blood parameters at the same time. The present study provides several useful norms for assessing the welfare of rainbow trout, indicating that the relationships among different parameters are important factors in interpreting the blood biochemical profiles.
Ice Nucleation Activity of Alpine Bioaerosol Emitted in Vicinity of a Birch Forest
Authors: Seifried Teresa M. , Bieber Paul , Kunert Anna T. , Schmale David G. , Whitmore Karin , Fröhlich-Nowoisky Janine , Grothe Hinrich
Published: June 2021

In alpine environments, many plants, bacteria, and fungi contain ice nuclei (IN) that control freezing events, providing survival benefits. Once airborne, IN could trigger ice nucleation in cloud droplets, influencing the radiation budget and the hydrological cycle. To estimate the atmospheric relevance of alpine IN, investigations near emission sources are inevitable. In this study, we collected 14 aerosol samples over three days in August 2019 at a single site in the Austrian Alps, close to a forest of silver birches, which are known to release IN from their surface. Samples were taken during and after rainfall, as possible trigger of aerosol emission by an impactor and impinger at the ground level. In addition, we collected aerosol samples above the canopy using a rotary wing drone. Samples were analyzed for ice nucleation activity, and bioaerosols were characterized based on morphology and auto-fluorescence using microscopic techniques. We found high concentrations of IN below the canopy, with a freezing behavior similar to birch extracts. Sampled particles showed auto-fluorescent characteristics and the morphology strongly suggested the presence of cellular material. Moreover, some particles appeared to be coated with an organic film. To our knowledge, this is the first investigation of aerosol emission sources in alpine vegetation with a focus on birches.
Neuropsychiatric and Cognitive Deficits in Parkinson’s Disease and Their Modeling in Rodents
Authors: Decourt Mélina , Jiménez-Urbieta Haritz , Benoit-Marand Marianne , Fernagut Pierre-Olivier
Published: June 2021

Parkinson’s disease (PD) is associated with a large burden of non-motor symptoms including olfactory and autonomic dysfunction, as well as neuropsychiatric (depression, anxiety, apathy) and cognitive disorders (executive dysfunctions, memory and learning impairments). Some of these non-motor symptoms may precede the onset of motor symptoms by several years, and they significantly worsen during the course of the disease. The lack of systematic improvement of these non-motor features by dopamine replacement therapy underlines their multifactorial origin, with an involvement of monoaminergic and cholinergic systems, as well as alpha-synuclein pathology in frontal and limbic cortical circuits. Here we describe mood and neuropsychiatric disorders in PD and review their occurrence in rodent models of PD. Altogether, toxin-based rodent models of PD indicate a significant but non-exclusive contribution of mesencephalic dopaminergic loss in anxiety, apathy, and depressive-like behaviors, as well as in learning and memory deficits. Gene-based models display significant deficits in learning and memory, as well as executive functions, highlighting the contribution of alpha-synuclein pathology to these non-motor deficits. Collectively, neuropsychiatric and cognitive deficits are recapitulated to some extent in rodent models, providing partial but nevertheless useful options to understand the pathophysiology of non-motor symptoms and develop therapeutic options for these debilitating symptoms of PD.