bluuebunny/crossref_metadata_2025 huggingface dataset:
Cognitive impairment in Chinese adult patients with type III spinal muscular atrophy without disease-modifying treatment
Authors: Hu Ying , Wei Ling , Li Aonan , Liu Tingting , Jiang Yubao , Xie Chengjuan , Wang Kai
Published: November 2023

Objective

Spinal muscular atrophy (SMA) is a neurodegenerative disorder characterized by the degeneration of motor neurons in the spinal cord. It remains uncertain whether the cognitive performance of adult patients with SMA is impaired. The objective of this study was to assess the cognitive profile of adult Chinese patients with SMA and the association between clinical features and cognitive ability, particularly executive function.

Methods

This cross-sectional study included 22 untreated adult patients with type III SMA and 20 healthy subjects. The following variables were assessed: general intelligence, memory, attention, language, executive function, depression, anxiety, and other demographic and clinical parameters. In addition, physical function was evaluated using the Hammersmith Functional Motor Scale Expanded (HFMSE), the Revised Upper Limb Module (RULM), and the 6-Minute Walk Test (6MWT).

Results

SMA patients had lower scores than healthy subjects in the Verbal Fluency Test, Stroop effect, Total Errors, Perseverative Responses, Perseverative Errors, and Non-perseverative Errors in the Wisconsin Card Sorting Test, showing impaired abilities of SMA patients in executive function. In the Attention Network Test (ANT), the results indicated that the SMA patients also had selective deficits in their executive control networks. Ambulant patients had better executive function test performance than non-ambulant ones. Compromised executive abilities in patients with SMA were correlated with a younger age at onset, poorer motor function, and higher levels of anxiety and depression.

Conclusion

Our study presented the distribution of cognitive impairment in a Chinese cohort with SMA. Patients with type III SMA showed selective deficits in executive function, which may be associated with disease severity, physical impairment, depression and anxiety. Future cognitive studies, accounting for motor and emotional impairment, are needed to evaluate if executive impairment is driven by specific brain changes or by those confounding factors.
Effects of soft robotic exoskeleton for gait training on clinical and biomechanical gait outcomes in patients with sub-acute stroke: a randomized controlled pilot study
Authors: Xie Ruimou , Zhang Yanlin , Jin Hainan , Yang Fei , Feng Yutong , Pan Yu
Published: November 2023

Background

Ankle function impairment is a critical factor impairing normal walking in survivors of stroke. The soft robotic exoskeleton (SRE) is a novel, portable, lightweight assistive device with promising therapeutic potential for gait recovery during post-stroke rehabilitation. However, whether long-term SRE-assisted walking training influences walking function and gait quality in patients following subacute stroke is unknown. Therefore, the primary objective of this study was to assess the therapeutic effects of SRE-assisted walking training on clinical and biomechanical gait outcomes in the rehabilitation of patients with subacute stroke.

Methods

A group patients who had experienced subacute stroke received conventional rehabilitation (CR) training combined with 10-session SRE-assisted overground walking training (30 min per session, 5 sessions/week, 2 weeks) (SRE group, n = 15) compared with the control group that received CR training only (CR group, n = 15). Clinical assessments and biomechanical gait quality measures were performed pre-and post-10-session intervention, with the 10-Minute Walk Test (10MWT) and 6-Minute Walk Test (6MWT) used to define the primary clinical outcome measures and the Functional Ambulation Category, Fugl-Meyer Assessment for Lower Extremity (FMA-LE) subscale, and Berg Balance Scale defined the secondary outcome measures. The gait quality outcome measures included spatiotemporal and symmetrical parameters during walking.

Results

After the 10-session intervention, the SRE and CR groups exhibited significant within-group improvements in all clinical outcome measures (p < 0.05). Between-comparison using covariance analyses demonstrated that the SRE group showed greater improvement in walking speed during the 10MWT (p < 0.01), distance walked during the 6MWT (p < 0.05), and FMA-LE scores (p < 0.05). Gait analyses showed that the SRE group exhibited significantly improved spatiotemporal symmetry (p < 0.001) after 10-session training, with no significant changes observed in the CR group.

Conclusion

Compared with CR training, SRE-assisted walking training led to greater improvements in walking speed, endurance, and motor recovery. Our findings provide preliminary evidence that SRE may be considered for inclusion in intensive gait training clinical rehabilitation programs to further improve walking function in patients who have experienced stroke.
Restoring autophagic function: a case for type 2 diabetes mellitus drug repurposing in Parkinson’s disease
Authors: Greco Marco , Munir Anas , Musarò Debora , Coppola Chiara , Maffia Michele
Published: November 2023

Parkinson’s disease (PD) is a predominantly idiopathic pathological condition characterized by protein aggregation phenomena, whose main component is alpha-synuclein. Although the main risk factor is ageing, numerous evidence points to the role of type 2 diabetes mellitus (T2DM) as an etiological factor. Systemic alterations classically associated with T2DM like insulin resistance and hyperglycemia modify biological processes such as autophagy and mitochondrial homeostasis. High glucose levels also compromise protein stability through the formation of advanced glycation end products, promoting protein aggregation processes. The ability of antidiabetic drugs to act on pathways impaired in both T2DM and PD suggests that they may represent a useful tool to counteract the neurodegeneration process. Several clinical studies now in advanced stages are looking for confirmation in this regard.
Positive or negative environmental modulations on human brain development: the morpho-functional outcomes of music training or stress
Authors: Mucignat-Caretta Carla , Soravia Giulia
Published: November 2023

In the last couple of decades, the study of human living brain has benefitted of neuroimaging and non-invasive electrophysiological techniques, which are particularly valuable during development. A number of studies allowed to trace the usual stages leading from pregnancy to adult age, and relate them to functional and behavioral measurements. It was also possible to explore the effects of some interventions, behavioral or not, showing that the commonly followed pathway to adulthood may be steered by external interventions. These events may result in behavioral modifications but also in structural changes, in some cases limiting plasticity or extending/modifying critical periods. In this review, we outline the healthy human brain development in the absence of major issues or diseases. Then, the effects of negative (different stressors) and positive (music training) environmental stimuli on brain and behavioral development is depicted. Hence, it may be concluded that the typical development follows a course strictly dependent from environmental inputs, and that external intervention can be designed to positively counteract negative influences, particularly at young ages. We also focus on the social aspect of development, which starts in utero and continues after birth by building social relationships. This poses a great responsibility in handling children education and healthcare politics, pointing to social accountability for the responsible development of each child.
Uptake of Pu(IV) by hardened cement paste in the presence of gluconate at high and low ionic strengths
Authors: Stietz J. , Amayri S. , Häußler V. , Prieur D. , Reich T.
Published: November 2023

The uptake of Pu(IV) by hardened cement paste (HCP) at degradation state I was investigated in the absence and presence of gluconate (GLU). Furthermore, the influence of the ionic strength was examined in different background electrolytes. Artificial cement pore water (ACW, pH = 13) was used for low ionic strength (I = 0.3 M), and cement pore water based on the diluted caprock solution (ACW-VGL, pH = 12.5) was used for high ionic strength (I = 2.5 M). Sorption experiments were performed under an Ar atmosphere using HCP in the HCP/GLU binary system ([GLU]0 = 1 × 10−1–1 × 10−8 M) and the HCP/Pu(IV)/GLU ternary system ([239Pu(IV)]0 = 1 × 10−8 M, [GLU]0 = 1 × 10−2 M) with solid-to-liquid (S/L) ratios of 0.5–50 g L–1 within a contact time of 72 h. GLU sorbs strongly on HCP; a saturation of the sorption sites of HCP with GLU was observed at [GLU] ≥ 1 × 10−4 M at S/L = 5 g L–1. The effects of the order of addition of the components Pu(IV) and GLU on the sorption of Pu(IV) on HCP were investigated. In the absence of GLU, a quantitative uptake (S% ≥ 99%) of Pu(IV) by HCP was observed, independent of the ionic strength of the background electrolytes. In the presence of 1 × 10−2 M GLU, the sorption of Pu(IV) on HCP was significantly lower. For X-ray absorption fine structure (XAFS) measurements, powder samples with Pu ([239Pu(III)]0 = 5 × 10−6 M) sorbed on HCP (S/L = 2.5 g L–1) were prepared at pH ≈ 13 in ACW and ACW-VGL, respectively. One additional sample was prepared in the presence of GLU ([GLU]0 = 1 × 10−2 M) with ACW-VGL as the electrolyte for comparison. Pu LIII-edge X-ray absorption near-edge structure (XANES) spectra show that Pu is in the tetravalent oxidation state after being taken up by the HCP. The structural parameters obtained from extended X-ray absorption fine structure (EXAFS) analysis and comparison with literature indicate incorporation of Pu(IV) into the calcium-silicate-hydrate (C-S-H) phases of HCP. The different ionic strengths and the presence of GLU had no influence on the near-neighbor environment of Pu in HCP.
The association of blood metals with latent tuberculosis infection among adults and adolescents
Authors: Wu Jinyi , Wang Kai , Tao Fengxi , Li Qingwen , Luo Xin , Xia Fang
Published: November 2023

Objective

We aimed to investigate the relationship of metal exposure and latent tuberculosis infection (LTBI) among US adults and adolescents.

Methods

Participants from the National Health and Nutrition Examination Surveys (NHANES 2011 ~ 2012) were included. Multiple logistic regression models were used to explore the associations between metal exposure and LTBI. A total of 5,248 adults and 1,860 adolescents were included in the present analysis.

Results

For adults, we only found a positive association between total mercury and LTBI (OR: 1.411; 95% CI: 1.164 ~ 1.710) when used as a continuous variable. Compared with Q1, Q4 increased the prevalence of LTBI (2.303; 1.455 ~ 3.644) when used as a quartile. The OR of total mercury and LTBI was higher among females (1.517; 1.009 ~ 2.279), individuals aged 45 ~ 64 (1.457; 1.060 ~ 2.002), and non-Hispanic White individuals (1.773; 1.316 ~ 2.388). A relationship was observed among only participants with obesity (1.553; 1.040 ~ 2.319) or underweight (1.380; 1.076 ~ 1.771), with college or above (1.645; 1.184 ~ 2.286), with PIR > 3.0 (1.701; 1.217 ~ 2.376), reported smoking (1.535; 1.235 ~ 1.907) and drinking (1.464; 1.232 ~ 1.739). For adolescents, blood manganese was positively associated with LTBI. The OR and 95% CIs for each one-unit increase in the log-transformed level of blood manganese with LTBI were 9.954 (1.389 ~ 71.344).

Conclusion

Significant associations were observed in girls, aged ≥12 years and in the non-Hispanic white population. In conclusion, total mercury is associated with an increased prevalence of LTBI among adults and positive association between blood manganese and LTBI was observed among adolescents. Further studies should be conducted to verify the results and explore potential biological mechanisms.
Advanced gastric cancer with metachronous intracranial oligometastases without recurrence after multidisciplinary team discussion and comprehensive treatment: a case report
Authors: Shao Lijuan , Zhang Hongyu , He Wenting , Wu Jun , Zhu Danxia , Cheng Haibo
Published: November 2023

This article describes the process of multidisciplinary team (MDT) discussion and comprehensive treatment of a case of advanced gastric cancer that tested positive for programmed death ligand 1 (PD-L1). During diagnosis, the patient presented with advanced gastric cancer and numerous unresectable metastases in the lesser omental lymph nodes, both lungs, liver, and left parietal occipital lobe. A meeting was arranged for the departments of oncology, gastrointestinal surgery, radiotherapy, imaging, and pathology to discuss the case. Initially, the patient had a partial response to the first-line treatment, which was a combination of pembrolizumab and chemotherapy. However, after nineteen months, the patient presented with a metachronous isolated lesion in the left frontal lobe. After mutual agreement among the oncology, brain surgery, gastrointestinal surgery, radiotherapy, imaging, and pathology departments, the intracranial lesion underwent resection. Following this, the operation was supplemented by stereotactic radiation therapy (SRT) and whole-brain radiation therapy (WBRT). The patient showed excellent signs of recovery after the operation, and her general condition remained favorable after 16 months of follow-up. Nonetheless, the outlook for patients facing advanced-stage gastric cancer remains distressing. Through multidisciplinary team (MDT) discussions, patients diagnosed with advanced gastric cancer can receive standardized diagnostic and treatment approaches to develop reasonable and personalized comprehensive treatment plans. Such plans help to improve the quality of life of patients and effectively prolong their survival time.
Acidification of intracellular pH in MM tumor cells overcomes resistance to hypoxia-mediated apoptosis in vitro and in vivo
Authors: Gastelum Gilberto , Kraut Jeffry , Veena Mysore , Baibussinov Alisher , Lamb Christopher , Lyons Kylee , Chang Eric Y. , Frost Patrick
Published: November 2023

Introduction

Multiple myeloma (MM) is an incurable cancer of malignant plasma cells that engraft in the bone marrow (BM). It is more than likely that the poorly investigated physical parameters of hypoxia and pH in the tumor microenvironment (TME) is critical for MM survival. Here, we explore the effects of a hypoxic environment on pH regulation and its role in MM survival.

Methods

We used in vitro models of MM, in which the culturing medium was modified to specific pH and pO2 levels and then measured the effects on cell survival that was correlated with changes in intracellular (pHi) and extracellular pH (pHe). In a MM xenograft model, we used PET/CT to study hypoxia-mediated effects on tumor growth.

Results

Hypoxia-mediated apoptosis of MM cells is correlated with acidic intracellular pHi (less than < 6.6) that is dependent on HIF activity. Using a polyamide HIF responsive element binding compound, a carbonic anhydrase inhibitor (acetazolamide), and an NHE-1 inhibitor (amiloride) acidified the pHi and lead to cell death. In contrast, treatment of cells with an alkalization agent, Na-lactate, rescued these cells by increasing the pHi (pH > 6.6). Finally, treatment of mice with acetazolamide decreased cell growth in the tumor nodules.

Discussion

Targeting hypoxia and HIF have been proposed as an anti-tumor therapy but the clinical efficacy of such strategies are modest. We propose that targeting the pHi may be more effective at treating cancers within a hypoxic TME.
Cholesterol in colorectal cancer: an essential but tumorigenic precursor?
Authors: He Xing , Lan Huanrong , Jin Ketao , Liu Fanlong
Published: November 2023

Colorectal cancer (CRC) is one of the most lethal human malignancies, and with the growth of societies and lifestyle changes, the rate of people suffering from it increases yearly. Important factors such as genetics, family history, nutrition, lifestyle, smoking, and alcohol can play a significant role in increasing susceptibility to this cancer. On the other hand, the metabolism of several macromolecules is also involved in the fate of tumors and immune cells. The evidence discloses that cholesterol and its metabolism can play a role in the pathogenesis of several cancers because there appears to be an association between cholesterol levels and CRC, and cholesterol-lowering drugs may reduce the risk. Furthermore, changes or mutations of some involved genes in cholesterol metabolism, such as CYP7A1 as well as signaling pathways, such as mitogen-activated protein kinase (MAPK), can play a role in CRC pathogenesis. This review summarized and discussed the role of cholesterol in the pathogenesis of CRC as well as available cholesterol-related therapeutic approaches in CRC.
Human behavior recognition based on sparse transformer with channel attention mechanism
Authors: Cao Keyan , Wang Mingrui
Published: November 2023

Human activity recognition (HAR) has recently become a popular research field in the wearable sensor technology scene. By analyzing the human behavior data, some disease risks or potential health issues can be detected, and patients’ rehabilitation progress can be evaluated. With the excellent performance of Transformer in natural language processing and visual tasks, researchers have begun to focus on its application in time series. The Transformer model models long-term dependencies between sequences through self-attention mechanisms, capturing contextual information over extended periods. In this paper, we propose a hybrid model based on the channel attention mechanism and Transformer model to improve the feature representation ability of sensor-based HAR tasks. Extensive experiments were conducted on three public HAR datasets, and the results show that our network achieved accuracies of 98.10%, 97.21%, and 98.82% on the HARTH, PAMAP2, and UCI-HAR datasets, respectively, The overall performance is at the level of the most advanced methods.
Discrepancies in resistant starch and starch physicochemical properties between rice mutants similar in high amylose content
Authors: Luo Mingrui , Gong Wanxin , Zhang Siyan , Xie Lanyu , Shi Yitao , Wu Dianxing , Shu Xiaoli
Published: November 2023

The content of resistant starch (RS) was considered positively correlated with the apparent amylose content (AAC). Here, we analyzed two Indica rice mutants, RS111 and Zhedagaozhi 1B, similar in high AAC and found that their RS content differed remarkably. RS111 had higher RS3 content but lower RS2 content than Zhedagaozhi 1B; correspondingly, cooked RS111 showed slower digestibility. RS111 had smaller irregular and oval starch granules when compared with Zhedagaozhi 1B and the wild type. Zhedagaozhi 1B showed a B-type starch pattern, different from RS111 and the wild type, which showed A-type starch. Meantime, RS111 had more fa and fb1 but less fb3 than Zhedagaozhi 1B. Both mutants showed decreased viscosity and swelling power when compared with the parents. RS111 had the lowest viscosity, and Zhedagaozhi 1B had the smallest swelling power. The different fine structures of amylopectin between RS111 and Zhedagaozhi 1B led to different starch types, gelatinization properties, paste viscosity, and digestibility. In addition to enhancing amylose content, modifications on amylopectin structure showed great potent in breeding rice with different RS2 and RS3 content, which could meet the increasing needs for various rice germplasms.
The relationship between teacher care behavior and EFL learning anxiety: the chain mediation effect of learning engagement and learning strategies
Authors: Wang Dongmei
Published: November 2023

This study aims to discuss the impact of teacher care behavior on EFL learning anxiety, as well as the mediating role of learning engagement and learning strategies. The Process plugin Model6 was used to measure the interaction between teacher care behavior (TCB), EFL learning anxiety (EFLLA), learning engagement (LE), and English learning strategies (ELS), in order to analyze and summarize their impact characteristics on college students’ EFL learning anxiety. The results show that teacher care behavior has a direct impact on EFL learning anxiety and a significant negative predictive effect on it. Learning engagement and English learning strategies play a mediating and chain mediating role between teacher care behavior and EFL learning anxiety. Thus, it can be seen that these factors can help reduce EFL learning anxiety, manifested in aspects such as mood, cognition, emotion, and behavior. Given the research findings, this study further provides suggestions for alleviating college students’ EFL learning anxiety, optimizing English teaching and learning design, and cultivating English learning strategies for college students.
A post-traumatic stress disorder among internally displaced people in sub-Saharan Africa: a systematic review
Authors: Koshe Tura , Jarso Mohammedamin Hajure , Walde Mandaras Tariku , Ebrahim Jemal , Mamo Aman , Esmael Adem , Wedajo Lema Fikadu , Seife Solomon , Mohammedhussein Mustefa , Nigatu Desalegn , Debele Gebiso Roba , Gezmu Wubishet
Published: November 2023

Introduction

Despite the prevalence of post-traumatic disorder in internally displaced persons, which is well established, and the fact that respective international organizations are working on the issues, little attention is given in the context of sub-Saharan Africa, This study aims to review the available data about the prevalence and determinants of post-traumatic stress disorders among internally displaced people in sub-Saharan Africa.

Methods

Studies published in the English language that have a clear outcome of interest and are available in full text were included. Six electronic databases were searched to identify published studies on the prevalence and determinants of posttraumatic stress disorder among IDPs in sub-Saharan Africa. This includes PubMed/MEDLINE, Scopus, EMBASE, PsychInfo, and the Web of Science. All relevant studies till June, 2023 were assessed. The review was done as per the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA-2009) and registered on PROSPERO (CRD420222997111).

Results

Originally, 33,138 articles were found in six databases, and finally, eleven studies were reviewed. The prevalence of post-traumatic stress disorder in sub-Saharan African countries was disproportionately presented in this review, ranging from 12.3% in Central Sudan to 85.5% in Nigeria. From a total of 11 studies, eight of them reported more than 50% of the magnitude of post-traumatic stress disorder, pointing to a higher magnitude of the prevalence of post-traumatic stress disorders in the region. The study identified numerous factors that contributed to post-traumatic stress disorder among the internally displaced population. Female gender, depression, anxiety, stress, being single, low level of educational status, experiencing or witnessing traumatic events, and psychological trauma were evidenced for their association with post-traumatic stress disorder.

Conclusion

These results demonstrate a higher prevalence of post-traumatic stress disorder compared to other regions of the world. The participants’ socio-demographic characteristics, including age, being single, being female, and a low level of education, were identified as factors contributing to PTSD. Moreover, the review identified that depression, anxiety, and experiencing or witnessing traumatic events were also influencing factors for PTSD among IDPs. The concerned bodies need to reinforce the monitoring and evaluation of the mental health programs of IDPs in the region.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=299711, CRD42022299711.
Effects of Linpan nature therapy on health benefits in older women with and without hypertension
Authors: Ye Xiang , Dou Zhiwen , Jiang Mingyan , Luo Zhenghua , Li Mao , Tang Haixiong , Huang Xiao , Wang Yuqian , Dong Liwei , Mao Xiaoguang , Feng Yu
Published: November 2023

Background

Nature therapy can significantly benefit the physiology and psychology of middle-aged and older people, but previous studies have focused on forest environments. The restoration potential of rural environments in urban fringe areas, which are more accessible to older people on a daily basis, has not been fully studied. This study assessed the effects of nature therapy on the physical and mental health of older women in a rural setting (locally known as Linpan) in the urban fringe area of Chengdu, China.

Methods

We recruited a total of 60 older women (65.3 ± 5.5 years old) living in cities for 3 days of nature therapy in the winter (30 subjects) and spring (30 subjects), including 20 hypertensive patients.

Results

The results showed that the overall blood pressure, pulse and sleep dysfunction rating scores of the participants were significantly lower than the pretest levels, and the finger blood oxygen saturation, mid-day salivary alpha-amylase and cortisol were increased post-treatment. Increases in these biomarker indicates and increase in stress. There were significant differences in the changes in systolic blood pressure between the hypertension group (HTN) and the normal group (normal) (HTN decreased by 8.8%, normal decreased by 5.4%), salivary alpha-amylase content (HTN decreased by 0.3%, normal increased by 16.9%), and sleep dysfunction rating scores (HTN decreased by 59.6%, normal decreased by 54%). The decreases in systolic blood pressure and pulse in the winter group were higher than those in the spring group by 1.8 and 4.4%, respectively, while the increases in salivary alpha-amylase content and salivary cortisol content were lower than those in the spring group by 11.7 and 11.2%, respectively, and the decrease in sleep dysfunction rating scores was lower than that in the spring group by 7.1%.

Conclusion

Our study concluded that nature therapy based on various health activities in the Linpan has significant health effects on older women. It can regulate blood pressure and pulse in older women, relieve cardiovascular disease, improve sleep quality. Meanwhile, older women with high blood pressure experienced a more significant effect than the healthy group.
Creativity as a framework for innovation in dental education
Authors: Byrne Samantha J. , Glasser Solange
Published: November 2023

Dental education is rich with examples of innovation as educators have responded to advances in knowledge, technology, the needs of the community, and most recently the challenges of the COVID-19 pandemic. Current challenges requiring innovative pedagogies include developing graduates who are interprofessional collaborative practice-ready, adapting to technological advances, embedding sustainability in the curriculum, and addressing equity and diversity in dental education. Creativity is the production of something that is novel and useful and is intimately linked to innovation which is the implementation of new and improved ways of doing things. To develop innovative pedagogies and address the current challenges facing dental education, educators and dental schools must reflect on the factors necessary for supporting creativity and innovation and seek to remove barriers to or biases against creativity. Here, we discuss the importance of creativity in supporting innovation in dental education, and call for leadership to actively support all elements of creativity for continued innovation to address the challenges we face in educating the future oral health workforce.
Assessment of glyphosate and its metabolites’ residue concentrations in cultured African Catfish offered for sale in selected markets in Ibadan, Oyo State, Nigeria
Authors: Alarape Selim Adewale , Fagbohun Adekemi Florence , Ipadeola Oladeni Adegoke , Adeigbo Anthony Ayodeji , Adesola Ridwan Olamilekan , Adeyemo Olanike Kudirat
Published: November 2023

Introduction: Glyphosate is a non-targeted organophosphate insecticide whose solubility and mobility in hydrophilic solvents enable its rapid leaching into the soil and subsequent contamination of ground and surface water and possible build-up in the aquatic food chain. Based on the public health importance of glyphosate in fish through consumption, it is crucial to determine the current residue concentration in culture Clarias gariepinus species. The aim of the present study is to evaluate glyphosate’s residue concentrations and its metabolites in cultured African Catfish offered for sale in selected markets in Ibadan.

Methods: A total of twenty-five (25) adult Clarias gariepinus (300 ± 50 g) were sourced from five (5) selected active fish markets (Ojoo, Iwo road, Eleyele, Challenge, and Apata) within the Ibadan metropolis. The collected fish tissue samples (liver, kidney, and spleen) were prepared for glyphosate residue concentration analysis using Liquid Chromatography (LC).

Results: The results showed that glyphosate residues were recorded in all the seventy-five (75) fish tissue samples obtained from the selected fish markets in the Ibadan metropolis and all residue concentrations were above both the recommended Acceptable Daily Intake (ADI) of 1.0 mg/kg (1 × 10−3 mg/L) and Maximum Residue Limits (MRL) of 0.01 mg/kg (1 × 10−5 mg/L). Isopropylamine has the highest residue concentration followed by N-Phosphonomethyl and Aminomethylphosphonic Acid (AMPA), while N-Acetyl Glyphosate has the least residue concentration across the sampled markets.

Discussion: The presence of residues of glyphosate and its metabolites in ready-to-eat fish calls for holistic, systematic, and effective risk management strategies towards monitoring pesticide/herbicide usage in aquaculture production and ensuring the provision of wholesome fish and fish products for the consumers.
Etiology, clinical presentation, and outcome of temporomandibular joint luxation in cats: 21 cases (2000–2018)
Authors: Jung Jin W. , Castejón-González Ana C. , Stefanovski Darko , Reiter Alexander M.
Published: November 2023

This study was performed to report etiology, clinical presentation, treatment, and outcome of temporomandibular joint (TMJ) luxation in 21 cats (2000–2018). TMJ luxation was diagnosed by either dental radiography or computed tomography (CT). Causes for TMJ luxation included altercation with a dog (38%), hit-by-car (19%), unknown (19%), fall (19%), and ran into inanimate object (5%). The most common complication was development of permanent malocclusion (24%), followed by reduced vertical mandibular range of motion (5%). Reduction of TMJ luxation was successful in 94.7% of the cases. Reduction of the TMJ luxation was significantly associated with time from injury to treatment. No other significant associations were observed between cause of injury, type of TMJ stabilization, and outcome. TMJ luxation in cats carries an excellent prognosis with early joint reduction and supportive care. Concurrent dental trauma and maxillofacial injuries are common, and all patients with head trauma must be stabilized and evaluated with a thorough examination.
Deep Dive into Fake News Detection: Feature-Centric Classification with Ensemble and Deep Learning Methods
Authors: Alarfaj Fawaz Khaled , Khan Jawad Abbas
Published: November 2023

The online spread of fake news on various platforms has emerged as a significant concern, posing threats to public opinion, political stability, and the dissemination of reliable information. Researchers have turned to advanced technologies, including machine learning (ML) and deep learning (DL) techniques, to detect and classify fake news to address this issue. This research study explores fake news classification using diverse ML and DL approaches. We utilized a well-known “Fake News” dataset sourced from Kaggle, encompassing a labelled news collection. We implemented diverse ML models, including multinomial naïve bayes (MNB), gaussian naïve bayes (GNB), Bernoulli naïve Bayes (BNB), logistic regression (LR), and passive aggressive classifier (PAC). Additionally, we explored DL models, such as long short-term memory (LSTM), convolutional neural networks (CNN), and CNN-LSTM. We compared the performance of these models based on key evaluation metrics, such as accuracy, precision, recall, and the F1 score. Additionally, we conducted cross-validation and hyperparameter tuning to ensure optimal performance. The results provide valuable insights into the strengths and weaknesses of each model in classifying fake news. We observed that DL models, particularly LSTM and CNN-LSTM, showed better performance compared to traditional ML models. These models achieved higher accuracy and demonstrated robustness in classification tasks. These findings emphasize the potential of DL models to tackle the spread of fake news effectively and highlight the importance of utilizing advanced techniques to address this challenging problem.
Diversity and Traits of Multiple Biotic Stressors Elicit Differential Defense Responses in Legumes
Authors: Basu Saumik , Moroz Natalia , Lee Benjamin W. , Tanaka Kiwamu , Oeller Liesl , Baerlocher Chase W. , Crowder David W.
Published: November 2023

In agroecosystems, plants frequently confront multiple biotic stressors, including herbivores and pathogens. The nature of these interactions plays a crucial role in mediating the activation of plant defense mechanisms. However, induction of plant chemical defenses has been more well studied than the induction of physical defenses. Here, we assessed the physical and chemical defense responses of pea (Pisum sativum) plants after exposure to three stressors: a vector herbivore (pea aphid, Acrythosiphon pisum), a non-vector herbivore (pea leaf weevil, Sitona lineatus), and a virus (Pea enation mosaic virus, PEMV). We used various histochemical staining techniques show that viruliferous A. pisum (transmitting PEMV) strongly induced callose deposition (aniline blue staining) and antioxidant-mediated defenses (DAB and NBT staining) in peas, primarily through accumulating reactive oxygen species (ROS). High-throughput phenotyping showed that viruliferous aphids reduced plant photosynthetic efficiency, but plants infected with PEMV had increased cell death (trypan blue staining). However, herbivory by aphids and weevils did not strongly induce defenses in peas, even though weevil feeding significantly reduced pea leaf area. These results show that not all herbivores induce strong defensive responses, and plant responses to vector species depends on their virus infection status. More broadly, our results indicate that variable stressors differentially regulate various plant responses through intricate chemical and physical defense pathways.
Effects of Gamma-Ray Irradiation of Bacteria Colonies in Animal Feeds and on Growth and Gut Health of Weaning Piglets
Authors: Wei Hao , Yang Min , Jiang Xuemei , Hua Lun , Jin Chao , Wu De , Wang Yan , Zhuo Yong
Published: November 2023

Animal feeds contain a substantial number and diversity of microorganisms, and some of them have pathogenic potential. The objectives of this study were to investigate the effects of different doses of gamma (γ)-ray irradiation on the bacteria count in different types of feed and then to test the effect of γ-ray-irradiation-treated fishmeal on the gut health and growth performance of weaning piglets. In trial 1, three fishmeal samples, two feather meal samples, three meat meal samples, three soybean meal samples, and three vitamin complexes were treated with γ-ray irradiation doses of 0, 3, 6, or 9 kGy. The 6 and 9 kGy doses eliminated most of the bacteria in the feed but also resulted in a loss of vitamin C and B1. In trial 2, 96 weaning piglets were fed one of the following three diets with eight replicates (pens) per group over a 14-day period: (1) the control diet—the basal diet supplemented with 6% fishmeal with a low bacteria count (40 CFU/g) and no E. coli; (2) the fishmeal-contaminated diet (FM-contaminated) diet—the basal diet supplemented with 6% fishmeal with a high bacteria count (91,500 CFU/g) and E. coli contamination; and (3) the irradiated fishmeal (irradiated FM) diet—the basal diet supplemented with γ-ray-irradiation-treated E. coli-contaminated fishmeal. The piglets that received the FM-contaminated diet had significantly lower average daily gain and a greater diarrhea index compared to those fed the control diet, whereas γ-ray irradiation treatment abrogated the negative effect of the E. coli-contaminated fishmeal. Collectively, γ-ray irradiation at a dose of 6–9 kGy was sufficient to eliminate the microorganisms in the feed, thereby benefitting the growth performance and gut health of the weaning piglets.
Elevated IgE Levels—An Allergy or an Underlying Inborn Error of Immunity in Children with Recurrent Infections?
Authors: Kostova Polina , Papochieva Vera , Miteva Dimitrinka , Georgieva Bilyana , Mileva Sirma , Shahid Martin , Lukanov Tsvetelin , Petrova Guergana
Published: November 2023

Elevated immunoglobulin E (IgE) is a hallmark of allergic diseases. However, high IgE levels also occur in a number of other infectious and noninfectious diseases. In most cases, elevated IgE levels indicate allergy, eczema, or chronic skin infection. Very high IgE levels are not uncommon in patients with active eczema but more often indicate monogenic atopic disorder or inborn errors of immunity with an atopic phenotype. We conducted a retrospective study of 385 children with suspected immune deficiency referred to the clinic over a 9-year period. Measurement of IgE, IgG, IgA, IgM, and IgG subclasses in blood samples revealed that nearly one-third of the patients had elevated serum IgE levels. Most of the cases with elevated IgE were children with underlying atopy—mainly atopic dermatitis and, to a lesser extent, bronchial asthma—whereas 40.12% (37 children) had no atopy at all. In the most severe cases (with extremely elevated IgE or severe dermatitis), we confirmed genetic mutations for underlying immunodeficiency. Our results indicate that allergic phenotype should not be underestimated and that children with more severe allergic disease should be evaluated for an underlying inborn error of immunity. If inborn error of immunity (IEI) is suspected, a comprehensive immunologic evaluation is required. Genetic testing helps identify the specific genetic abnormality, which provides important insight into the immunopathogenesis of the disease and accurate determination of optimal therapy.
Trends in the Development of Antibody-Drug Conjugates for Cancer Therapy
Authors: Song Chi Hun , Jeong Minchan , In Hyukmin , Kim Ji Hoe , Lin Chih-Wei , Han Kyung Ho
Published: November 2023

In cancer treatment, the first-generation, cytotoxic drugs, though effective against cancer cells, also harmed healthy ones. The second-generation targeted cancer cells precisely to inhibit their growth. Enter the third-generation, consisting of immuno-oncology drugs, designed to combat drug resistance and bolster the immune system’s defenses. These advanced therapies operate by obstructing the uncontrolled growth and spread of cancer cells through the body, ultimately eliminating them effectively. Within the arsenal of cancer treatment, monoclonal antibodies offer several advantages, including inducing cancer cell apoptosis, precise targeting, prolonged presence in the body, and minimal side effects. A recent development in cancer therapy is Antibody-Drug Conjugates (ADCs), initially developed in the mid-20th century. The second generation of ADCs addressed this issue through innovative antibody modification techniques, such as DAR regulation, amino acid substitutions, incorporation of non-natural amino acids, and enzymatic drug attachment. Currently, a third generation of ADCs is in development. This study presents an overview of 12 available ADCs, reviews 71 recent research papers, and analyzes 128 clinical trial reports. The overarching objective is to gain insights into the prevailing trends in ADC research and development, with a particular focus on emerging frontiers like potential targets, linkers, and drug payloads within the realm of cancer treatment.
Prevalence of Multidrug-Resistant and ESBL-Producing Bacterial Pathogens in Patients with Chronic Wound Infections and Spinal Cord Injury Admitted to a Tertiary Care Rehabilitation Hospital
Authors: Binsuwaidan Reem , Khan Mohammad Aatif , Alzahrani Raghad H. , Aldusaymani Aljoharah M. , Almallouhi Noura M. , Alsabti Alhanouf S. , Ali Sajjad , Khan Omar Sufyan , Youssef Amira M. , Alnajjar Lina I.
Published: November 2023

A pressure ulcer is defined as a skin lesion of ischemic origin, a condition that contributes to morbidity and mortality in patients with spinal cord injuries. The most common complication of ulcers is a bacterial infection. Antimicrobial therapy should be selected with caution for spinal cord injury patients since they have a high risk of developing multidrug-resistant (MDR) infections. The aim of this study was to determine the prevalence of different bacterial pathogens in patients with pressure ulcers admitted with spinal cord injuries. This was a retrospective single-center study that included adult patients aged 18 years and above, admitted with chronic pressure wounds after a spinal cord injury requiring hospitalization between 2015 and 2021. A total of 203 spinal cord injury patients with pressure ulcers were included in the study. Ulcers were commonly infected by Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, and they were mostly located in the sacral and gluteal areas. More than half of the bacteria isolated from patients were sensitive to commonly tested antibiotics, while 10% were either MDR- or pan-drug-resistant organisms. Of the MDR bacterial isolates, 25.61% were methicillin-resistant S. aureus, and 17.73% were extended-spectrum beta-lactamase Enterobacteriaceae. The most prevalent bacteria in pressure ulcers of spinal cord injury patients were S. aureus. Other antibiotic-resistant organisms were also isolated from the wounds.
Traditional Cultures versus Next Generation Sequencing for Suspected Orthopedic Infection: Experience Gained from a Reference Centre
Authors: Rimoldi Sara Giordana , Brioschi Davide , Curreli Daniele , Salari Federica , Pagani Cristina , Tamoni Alessandro , Longobardi Concetta , Bosari Raffaella , Rizzo Alberto , Landonio Simona , Coen Massimo , Passerini Matteo , Gismondo Maria Rita , Gori Andrea , Manzotti Alfonso
Published: November 2023

(Background) The diagnosis and the antimicrobial treatment of orthopedic infection are challenging, especially in cases with culture-negative results. New molecular methods, such as next-generation sequencing (NGS), promise to overcome some limitations of the standard culture, such as the detection of difficult-to-grow bacteria. However, data are scarce regarding the impact of molecular techniques in real-life scenarios. (Methods) We included cases of suspected orthopedic infection treated with surgery from May 2021 to September 2023. We combined traditional cultures with NGS. For NGS, we performed a metagenomic analysis of ribosomal 16s, and we queried dedicated taxonomic libraries to identify the species. To avoid false positive results, we set a cut-off of 1000 counts of the percentage of frequency of reads. (Results) We included 49 patients in our study. Our results show the presence of bacteria in 36/49 (73%) and 29/49 (59%) cases studied with NGS and traditional cultures, respectively. The concordance rate was 61%. Among the 19/49 discordant cases, in 11/19 cases, cultures were negative and NGS positive; in 4/19, cultures were positive and NGS negative; and in the remaining 4/19, different species were detected by traditional cultures and NGS. (Conclusions) Difficult-to-grow microorganisms, such as slow-growing anaerobic bacteria, were better detected by NGS compared to traditional culture in our study. However, more data to distinguish between true pathogens and contaminants are needed. NGS can be an additional tool to be used for the diagnosis of orthopedic infections and the choice of appropriate antimicrobial therapy.
Fusion of Attention-Based Convolution Neural Network and HOG Features for Static Sign Language Recognition
Authors: Kumari Diksha , Anand Radhey Shyam
Published: November 2023

The deaf and hearing-impaired community expresses their emotions, communicates with society, and enhances the interaction between humans and computers using sign language gestures. This work presents a strategy for efficient feature extraction that uses a combination of two different methods that are the convolutional block attention module (CBAM)-based convolutional neural network (CNN) and standard handcrafted histogram of oriented gradients (HOG) feature descriptor. The proposed framework aims to enhance accuracy by extracting meaningful features and resolving issues like rotation, similar hand orientation, etc. The HOG feature extraction technique provides a compact feature representation that signifies meaningful information about sign gestures. The CBAM attention module is incorporated into the structure of CNN to enhance feature learning using spatial and channel attention mechanisms. Then, the final feature vector is formed by concatenating these features. This feature vector is provided to the classification layers to predict static sign gestures. The proposed approach is validated on two publicly available static Massey American Sign Language (ASL) and Indian Sign Language (ISL) databases. The model’s performance is evaluated using precision, recall, F1-score, and accuracy. Our proposed methodology achieved 99.22% and 99.79% accuracy for the ASL and ISL datasets. The acquired results signify the efficiency of the feature fusion and attention mechanism. Our network performed better in accuracy compared to the earlier studies.
Technological Aspects and Performance of High Entropy Alloys with Potential Application in Dental Restorations and Reducing Implant Failure
Authors: Demetrescu Ioana , Nartita Radu , Andrei Mihai , Didilescu Andreea Cristiana , Cimpean Anisoara , Ionita Daniela
Published: November 2023

Amidst the prevalence of aggressive bacterial infections that can impact both oral and systemic health following various dental and implant procedures, the search for alternative, high-performing and biocompatible materials has become a challenging pursuit. The need for such investigations is increasing owing to the fact that toxicological risks of cobalt–chromium (CoCr) alloys used in dentistry have become a part of the EU’s new Medical Devices Regulations establishing that cobalt metal has been classified as carcinogenic, genotoxic and detrimental to reproduction. Within this context, this review proposes high entropy alloys (HEA) as potential alternatives and presents their characteristics and in vitro biological performance when used as a substrate or coating. Anatomical details of the oral cavity in relationship with prosthodontics and implant dentistry support the paper’s motivation and presentation. The review highlights the innovative manufacturing procedures, microstructure and properties of both the bulk and coatings of BioHEA. It evaluates the performance of BioHEAs based on their complete characterization and assesses their suitability for novel applications in dentistry, serving as the primary objective of this manuscript.
SSDLiteX: Enhancing SSDLite for Small Object Detection
Authors: Kang Hyeong-Ju
Published: November 2023

Object detection in many real applications requires the capability of detecting small objects in a system with limited resources. Convolutional neural networks (CNNs) show high performance in object detection, but they are not adequate to resource-limited environments. The combination of MobileNet V2 and SSDLite is one of the common choices in such environments, but it has a problem in detecting small objects. This paper analyzes the structure of SSDLite and proposes variations leading to small object detection improvement. The feature maps with the higher resolution are utilized more, and the base CNN is modified to have more layers in the high resolution. Experiments have been performed for the various configurations and the results show the proposed CNN, SSDLiteX, improves the detection accuracy AP of small objects by 1.5 percent points in the MS COCO data set.