bluuebunny/crossref_metadata_2025 huggingface dataset:
The Association Between Affective Temperament Traits and Dopamine Genes in Obese Population
Authors: Lesiewska Natalia , Borkowska Alina , Junik Roman , Kamińska Anna , Pulkowska-Ulfig Joanna , Tretyn Andrzej , Bieliński Maciej
Published: April 2019

Studies indicate the heritable nature of affective temperament, which shows personality traits predisposing to the development of mental disorders. Dopaminergic gene polymorphisms such as DRD4, COMTVal158Met, and DAT1 have been linked to affective disorders in obesity. Due to possible correlation between the aforementioned polymorphisms and the affective temperament, the aim of our research was to investigate this connection in an obese population. The study enrolled 245 obese patients (178 females; 67 males). The affective temperament was assessed using the Temperament Evaluation of Memphis, Pisa, Paris, and San Diego autoquestionnaire (TEMPS-A). Genetic polymorphisms of DAT1, COMTVal158Met and DRD4 were collected from peripheral blood sample and determined using a polymerase chain reaction (PCR). Only in COMT polymorphisms, the cyclothymic and irritable dimensions were significantly associated with Met/Val carriers (p = 0.04; p = 0.01). Another interesting finding was the correlation between the affective temperament and age in men and women. We assume that dopamine transmission in heterozygotes of COMT may determine the role of the affective temperament in obese persons. Dopaminergic transmission modulated by COMT may be responsible for a greater temperament expression in obese individuals. To our knowledge, this is the first study describing the role of affective temperament in the obese population, but more research is needed in this regard.
Intermittent Hypoxia Up-Regulates Gene Expressions of Peptide YY (PYY), Glucagon-like Peptide-1 (GLP-1), and Neurotensin (NTS) in Enteroendocrine Cells
Authors: Shobatake Ryogo , Itaya-Hironaka Asako , Yamauchi Akiyo , Makino Mai , Sakuramoto-Tsuchida Sumiyo , Uchiyama Tomoko , Ota Hiroyo , Takahashi Nobuyuki , Ueno Satoshi , Sugie Kazuma , Takasawa Shin
Published: April 2019

The patients with sleep apnea syndrome are exposed to intermittent hypoxia (IH) during sleep. We previously demonstrated the IH-induced up-regulation of the mRNA levels of anorexigenic peptides proopiomelanocortin (POMC), and cocaine- and amphetamine-regulated transcript (CART) in human neuronal cells. Appetite is regulated not only by the central nervous system but also by the peptides from gastrointestinal tract. Here, we investigated the effects of IH on the gene expression(s) of appetite-inhibiting gut hormones. Human enteroendocrine Caco-2 and mouse STC-1 cells were exposed to IH [64 cycles of 5 min hypoxia (1% O2) and 10 min normoxia (21% O2)] or normoxia for 24 h. Real-time RT-PCR revealed that IH significantly increased the mRNA levels of peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NTS) in Caco-2 and STC-1 cells. ELISA showed that the concentrations of PYY, GLP-1, and NTS in the culture medium were significantly increased by IH. The mRNA levels of PYY, GLP-1, and NTS were significantly up-regulated even in normoxia by Trichostatin A (TSA) and were significantly decreased even in IH by 5-azacytidine (5AZC), suggesting that IH increases PYY, GLP-1, and NTS mRNAs via alterations in the chromatin structure in enteroendocrine cells. IH might have an anorexigenic influence on the enteric nervous system.
Thrombomodulin Regulation of Mitogen-Activated Protein Kinases
Authors: Giri Hemant , Cai Xiaofeng , Panicker Sumith R. , Biswas Indranil , Rezaie Alireza R.
Published: April 2019

The multifaceted role of mitogen-activated protein kinases (MAPKs) in modulating signal transduction pathways in inflammatory conditions such as infection, cardiovascular disease, and cancer has been well established. Recently, coagulation factors have also emerged as key players in regulating intracellular signaling pathways during inflammation. Among coagulation factors, thrombomodulin, as a high affinity receptor for thrombin on vascular endothelial cells, has been discovered to be a potent anti-inflammatory and anti-tumorigenic signaling molecule. The protective signaling function of thrombomodulin is separate from its well-recognized role in the clotting cascade, which is to function as an anti-coagulant receptor in order to switch the specificity of thrombin from a procoagulant to an anti-coagulant protease. The underlying protective signaling mechanism of thrombomodulin remains largely unknown, though a few published reports link the receptor to the regulation of MAPKs under different (patho)physiological conditions. The goal of this review is to summarize what is known about the regulatory relationship between thrombomodulin and MAPKs.
Circadian Analysis of the Mouse Cerebellum Proteome
Authors: Plumel Marine , Dumont Stéphanie , Maes Pauline , Sandu Cristina , Felder-Schmittbuhl Marie-Paule , Challet Etienne , Bertile Fabrice
Published: April 2019

The cerebellum contains a circadian clock, generating internal temporal signals. The daily oscillations of cerebellar proteins were investigated in mice using a large-scale two-dimensional difference in gel electrophoresis (2D-DIGE). Analysis of 2D-DIGE gels highlighted the rhythmic variation in the intensity of 27/588 protein spots (5%) over 24 h based on cosinor regression. Notably, the rhythmic expression of most abundant cerebellar proteins was clustered in two main phases (i.e., midday and midnight), leading to bimodal distribution. Only six proteins identified here to be rhythmic in the cerebellum are also known to oscillate in the suprachiasmatic nuclei, including two proteins involved in the synapse activity (Synapsin 2 [SYN2] and vesicle-fusing ATPase [NSF]), two others participating in carbohydrate metabolism (triosephosphate isomerase (TPI1] and alpha-enolase [ENO1]), Glutamine synthetase (GLUL), as well as Tubulin alpha (TUBA4A). Most oscillating cerebellar proteins were not previously identified in circadian proteomic analyses of any tissue. Strikingly, the daily accumulation of mitochondrial proteins was clustered to the mid-resting phase, as previously observed for distinct mitochondrial proteins in the liver. Moreover, a number of rhythmic proteins, such as SYN2, NSF and TPI1, were associated with non-rhythmic mRNAs, indicating widespread post-transcriptional control in cerebellar oscillations. Thus, this study highlights extensive rhythmic aspects of the cerebellar proteome.
Influence of Cigarette Smoke Inhalation on an Autogenous Onlay Bone Graft Area in Rats with Estrogen Deficiency: A Histomorphometric and Immunohistochemistry Study
Authors: Moretto Nunes Camilla Magnoni , Bernardo Daniella Vicensotto , Ferreira Camila Lopes , Gomes Mônica Fernandes , De Marco Andrea Carvalho , Santamaria Mauro Pedrine , Jardini Maria Aparecida Neves
Published: April 2019

Purpose: The present study aimed to evaluate the influence of cigarette smoke inhalation on an autogenous onlay bone graft area, either covered with a collagen membrane or not, in healthy and estrogen-deficient rats through histomorphometry and immunohistochemistry. Materials and Methods: Sixty female rats (Wistar), weighing 250–300 g, were randomly divided and allocated into groups (either exposed to cigarette smoke inhalation or not, ovariectomized and SHAM). After 15 days, the test group underwent cigarette smoke inhalation. Sixty days after exposition, autogenous bone grafting was only performed on all right hemimandibles, and the left ones underwent autogenous onlay bone grafting with the collagen membrane (BioGide®). The graft was harvested from the parietal bone and attached to the animals’ jaws (right and left). They were euthanized at 21, 45, and 60 days after grafting. Histological measurements and immunohistochemical analyses were performed, and results were submitted to a statistical analysis. Results: The addition of a collagen membrane to the bone graft proved more efficient in preserving graft area if compared to the graft area without a collagen membrane and the one associated with cigarette smoke inhalation at 21 (p = 0.0381) and 60 days (p = 0.0192), respectively. Cigarette smoke inhalation combined with ovariectomy promoted a significant reduction of the autogenous graft area at 21 and 60 days. At 45 days, no statistically significant results were observed. In the immunohistochemical analysis, the ovariectomized and smoking subgroups, combined or not with collagen membrane, received moderate and intense immunolabeling at 21 days for Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) (p = 0.0017 and p = 0.0381, respectively). For Osteoprotegerin (OPG), intense immunolabeling was observed in most subgroups under analysis at 60 days. Conclusion: Smoking inhalation promoted resorption on the autogenous onlay bone graft, mainly when associated with ovariectomy. Furthermore, when associated with the collagen membrane, a lower resorption rate was observed if compared to the absence of the membrane.
Green Ferrate(VI) for Multiple Treatments of Fracturing Wastewater: Demulsification, Visbreaking, and Chemical Oxygen Demand Removal
Authors: Han Hongjing , Li Jinxin , Ge Qin , Wang Yizhen , Chen Yanguang , Wang Baohui
Published: April 2019

Fracturing wastewater is often highly emulsified, viscous, and has a high chemical oxygen demand (COD), which makes it difficult to treat and recycle. Ferrate(VI) is a green oxidant that has a high redox potential and has been adopted for the efficient oxidation of fracturing wastewater to achieve triple effects: demulsification, visbreaking, and COD removal. Firstly, optimal conditions were identified to build a model for fast and efficient treatment. Secondly, wastewater treatment using ferrate oxidation was investigated via demulsification, visbreaking, and COD removal. Finally, a mechanism for ferrate oxidation was proposed for the three effects using Fourier-transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The theoretical and experimental data demonstrated that the ferrate oxidation achieved the three desired effects. When ferrate was added, the demulsification efficiency increased from 56.2% to 91.8%, the total viscosity dropped from 1.45 cp to 1.10 cp, and the total removal rate of COD significantly increased to 74.2%. A mechanistic analysis showed that the strongly-oxidizing ferrate easily and efficiently oxidized the O/W interfacial film materials, viscous polymers, and compounds responsible for the COD, which was a promising result for the triple effects.
Green Tea Seed Oil Suppressed Aβ1–42-Induced Behavioral and Cognitive Deficit via the Aβ-Related Akt Pathway
Authors: Kim Jong Min , Park Seon Kyeong , Kang Jin Yong , Park Su Bin , Yoo Seul Ki , Han Hye Ju , Cho Kyoung Hwan , Kim Jong Cheol , Heo Ho Jin
Published: April 2019

The aim of this study was to investigate the availability of seeds, one of the byproducts of green tea, and evaluate the physiological activity of seed oil. The ameliorating effect of green tea seed oil (GTO) was evaluated on H2O2-induced PC12 cells and amyloid beta (Aβ)1–42-induced ICR mice. GTO showed improvement of cell viability and reduced reactive oxygen species (ROS) production in H2O2-induced PC12 cells by conducting the 2′,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 2′,7′-dichlorofluorescein diacetate (DCF-DA) analysis. Also, administration of GTO (50 and 100 mg/kg body weight) presented protective effects on behavioral and memory dysfunction by conducting Y-maze, passive avoidance, and Morris water maze tests in Aβ-induced ICR mice. GTO protected the antioxidant system by reducing malondialdehyde (MDA) levels, and by increasing superoxide dismutase (SOD) and reducing glutathione (GSH) contents. It significantly regulated the cholinergic system of acetylcholine (ACh) contents, acetylcholinesterase (AChE) activities, and AChE expression. Also, mitochondrial function was improved through the reduced production of ROS and damage of mitochondrial membrane potential (MMP) by regulating the Aβ-related c-Jun N-terminal kinase (JNK)/protein kinase B (Akt) and Akt/apoptosis pathways. This study suggested that GTO may have an ameliorating effect on cognitive dysfunction and neurotoxicity through various physiological activities.
Learning Subword Embedding to Improve Uyghur Named-Entity Recognition
Authors: Saimaiti Alimu , Wang Lulu , Yibulayin Tuergen
Published: April 2019

Uyghur is a morphologically rich and typical agglutinating language, and morphological segmentation affects the performance of Uyghur named-entity recognition (NER). Common Uyghur NER systems use the word sequence as input and rely heavily on feature engineering. However, semantic information cannot be fully learned and will easily suffer from data sparsity arising from morphological processes when only the word sequence is considered. To solve this problem, we provide a neural network architecture employing subword embedding with character embedding based on a bidirectional long short-term memory network with a conditional random field layer. Our experiments show that subword embedding can effectively enhance the performance of the Uyghur NER, and the proposed method outperforms the model-based word sequence method.
Electronic Structure of Cubane-Like Vanadium–Nitrogen Cationic Clusters [V4N4]+ and [V6N6]+
Authors: He Piao , Zhang Jian-Guo , McGrady John E.
Published: April 2019

Density Functional Theory and Complete Active Space Self-Consistent Field (CASSCF) methodologies are used to explore the electronic structure of the cationic V–N clusters, [V4N4]+ and [V6N6]+, that have been identified in recent mass spectrometric experiments. Our calculations indicate that both clusters are based on cubane-like fragments of the rock-salt lattice. In the smaller [V4N4]+ cluster, the V–V bonding is delocalized over the tetrahedron, with net bond orders of 1/3 per V–V bond. In [V6N6]+, in contrast, the V–V bonding is strongly localized in the central V2N2 unit, which has a short V=V double bond. CASSCF calculations reveal that both localized and delocalized V–V bonds are highly multi-configurational.
A Multivariate Evaluation of 25 Proximal and Distal Risk-Factors for Gambling-Related Harm
Authors: Browne Matthew , Hing Nerilee , Rockloff Matthew , Russell Alex M. T. , Greer Nancy , Nicoll Fiona , Smith Garry
Published: April 2019

Individual differences in the risk of developing gambling-related harm play an important role in theoretical models and practical interventions. The present study attempted comprehensive measurement and evaluation of 25 known risk factors for gambling-related harm in order to determine which factors provided large and unique explanatory power. We surveyed 1650 regular gamblers from an online panel, screening in 1174 (466 male) who passed all checks of attention and response consistency. We evaluated each risk factor based on bivariate correlations with harms, then made separate multivariate evaluations of proximal (e.g., gambling motivations) and distal (e.g., religiosity) risk factors. Almost all bivariate correlations were significant, but most distal factors were not significant in multivariate models. Trait impulsivity was the most important risk factor by a large margin. Excessive consumption, less use of safe gambling practices, and more fallacies were key proximal risks of harm. Many well-known correlates of gambling harm (e.g., youth, lower educational attainment) do not show a direct role in the development of gambling harm when controlling for other factors. The results support theoretical models that emphasise early conditioning and biological vulnerability (manifested through impulsivity). Since maladaptive cognitive and behavioural schemas appear to be more important than motivations (e.g., escape, excitement, ego), interventions may benefit by targeting these proximal drivers of harm.
Stability Analysis of Near-Wellbore Reservoirs Considering the Damage of Hydrate-Bearing Sediments
Authors: Zhang Xiaoling , Xia Fei , Xu Chengshun , Han Yan
Published: April 2019

The stability of hydrate-bearing near-wellbore reservoirs is one of the key issues in gas hydrate exploitation. In most previous investigations, the damage evolution process of the sediment structure and its effect on near-wellbore reservoir stability have been neglected. Therefore, the damage variable is introduced into a multi-field coupled model based on continuous damage theory and multi-field coupling theory. A thermo-hydro-mechanical-chemical (THMC) multi-field coupling mathematical model considering damage of hydrate-bearing sediments is established. The effects of damage of hydrate-bearing sediments on the thermal field, seepage field, and mechanical field are considered. Finally, the distributions of hydrate saturation, pore pressure, damage variable, and effective stress of a near-wellbore reservoir in gas hydrate exploitation by depressurization are calculated, and the stability of a hydrate-bearing near-wellbore reservoir is analyzed using the model. Through calculation and analysis, it is found that structural damage of hydrate-bearing sediments has an adverse effect on the stability of hydrate-bearing near-wellbore reservoirs. The closer to the wellbore, the worse the reservoir stability, and the near-wellbore reservoir stability is the worst in the direction of minimum horizontal ground stress.
Implementing Green Infrastructure in Spatial Planning in Europe
Authors: Slätmo Elin , Nilsson Kjell , Turunen Eeva
Published: April 2019

Interest in green infrastructure (GI) has grown in research, policy and planning in recent decades. The central idea behind GI is the understanding of the physical non-built-up environment as an infrastructure capable of delivering a wide variety of benefits to society, including the ability to preserve biodiversity; to provide food, feed, fuel and fibre; to adapt to and mitigate climate change and to contribute to enhanced human health and quality of life. The European Union (EU) has had a GI strategy since 2013, and member states are involved in several strategic and applied GI initiatives and projects. The aim of this study is to explore if and how the European strategy has been implemented. The study adds to the body of knowledge of current GI policies and measures in Europe via an online survey and insights into previous research. The survey reveals that GI is integrated into one or more policy sectors in all 32 countries covered. In 11 of the 32 countries, GI-specific policies are already in place or are being drawn up at a national level. In general, the respondents see the responsibility for GI policy and strategy as a matter of national governments and the implementation as a matter of local governments. They also see the LIFE+ and Horizon 2020 project funds, the European Regional Development Fund (ERDF) and the European Agricultural Fund for Rural Development (EAFRD), as the most important EU funding sources for the implementation of the GI strategy. The study also identifies availability of georeferenced information, zoning and biotope area factor as three of the spatial planning tools used to implement GI.
Performance Analysis and Soil Quality Indexing for Dalbergia sissoo Roxb. Grown in Marginal and Degraded Land of Eastern Uttar Pradesh, India
Authors: Edrisi Sheikh Adil , Tripathi Vishal , Abhilash Purushothaman Chirakkuzhyil
Published: April 2019

The successful utilization of marginal and degraded lands for biomass and bioenergy production depends upon various factors such as climatic conditions, the adaptive traits of the tree species and their growth rate and respective belowground responses. The present study was undertaken to evaluate the growth performance of a bioenergy tree (Dalbergia sissoo Roxb.) grown in marginal and degraded land of the Mirzapur district of Uttar Pradesh, India and to analyze the effect of D. sissoo plantations on soil quality improvement over the study years. For this, a soil quality index (SQI) was developed based on principal component analysis (PCA) to understand the effect of D. sissoo plantations on belowground responses. PCA results showed that among the studied soil variables, bulk density (BD), moisture content (MC), microbial biomass carbon (MBC) and soil urease activity (SUA) are the key variables critically influencing the growth of D. sissoo. The SQI was found in an increasing order with the growth period of D. sissoo. (i.e., from 0.419 during the first year to 0.579 in the fourth year). A strong correlation was also observed between the growth attributes (diameter at breast height, R2 = 0.870; and plant height, R2 = 0.861) and the soil quality (p < 0.01). Therefore, the developed SQI can be used as key indicator for monitoring the restoration potential of D. sissoo growing in marginal and degraded lands and also for adopting suitable interventions to further improve soil quality for multipurpose land restoration programs, thereby attaining land degradation neutrality and United Nations Sustainable Development Goals.
Thermo–Mechanical Behavior and Constitutive Modeling of In Situ TiB2/7050 Al Metal Matrix Composites Over Wide Temperature and Strain Rate Ranges
Authors: Lin Kunyang , Wang Wenhu , Jiang Ruisong , Xiong Yifeng , Shan Chenwei
Published: April 2019

The thermo–mechanical behavior of in situ TiB2/7050 Al metal matrix composites is investigated by quasi-static and Split Hopkinson Pressure Bar compression tests over a wide range of temperature (20~30 °C) and strain rate (0.001~5000 s−1). Johnson–Cook and Khan–Liu constitutive models determined from curve fitting and constrained optimization are used to predict the flow stress during deformation. In addition, another Johnson–Cook model calculated from an orthogonal cutting experiment and finite element simulation is also compared in this study. The prediction capability of these models is compared in terms of correlation coefficient and average absolute error. Due to the assumptions in orthogonal cutting theory, the determined Johnson–Cook model from cutting cannot describe the material deformation behavior accurately. The results also show that the Khan–Liu model has better performance in characterizing the material’s thermo–mechanical behavior.
Hydrometallurgical Process for Tantalum Recovery from Epoxy-Coated Solid Electrolyte Tantalum Capacitors
Authors: Chen Wei-Sheng , Ho Hsing-Jung , Lin Kuan-Yan
Published: April 2019

Tantalum is a critical metal that is widely used in electronic products. The demand for tantalum is increasing, but the supply is limited. As tantalum waste products have increased in Taiwan in recent years, the treatment of spent tantalum capacitors has become necessary and important. The recycling of tantalum from tantalum capacitors will not only decrease pollution from waste, but will also conserve tantalum resources. The tantalum content in epoxy-coated solid electrolyte tantalum capacitors (EcSETCs) is over 40 wt.%. Here, we designed a recycling process that includes pre-treatment, leaching, and solvent extraction to recover tantalum. In the pre-treatment process, epoxy resin and wires were removed. During hydrometallurgical process, pressure leaching by hydrofluoric acid was used to leach tantalum and manganese from solid electrolyte tantalum capacitors (SETCs). During our testing of this proposed process, the acid concentration, reaction time, temperature, and solid–liquid ratio were examined for leaching. After the leaching process, Alamine 336 was used to extract tantalum from the leaching solution. The pH value, extractant concentration, extraction time, and aqueous–organic ratio were investigated. Then, tantalum was stripped using HNO3, and the HNO3 concentration, stripping time, and organic–aqueous ratio were analyzed in detail. Under optimal conditions, the recovery efficiency of tantalum reached over 98%, and a final product of tantalum pentoxide with 99.9% purity was obtained after chemical precipitation and calcination.
The Influence of Microstructure on the Passive Layer Chemistry and Corrosion Resistance for Some Titanium-Based Alloys
Authors: El-Bagoury Nader , Ahmed Sameh I. , Ahmed Abu Ali Ola , El-Hadad Shimaa , Fallatah Ahmed M. , Mersal G. A. M. , Ibrahim Mohamed M. , Wysocka Joanna , Ryl Jacek , Boukherroub Rabah , A. Amin Mohammed
Published: April 2019

The effect of microstructure and chemistry on the kinetics of passive layer growth and passivity breakdown of some Ti-based alloys, namely Ti-6Al-4V, Ti-6Al-7Nb and TC21 alloys, was studied. The rate of pitting corrosion was evaluated using cyclic polarization measurements. Chronoamperometry was applied to assess the passive layer growth kinetics and breakdown. Microstructure influence on the uniform corrosion rate of these alloys was also investigated employing dynamic electrochemical impedance spectroscopy (DEIS). Corrosion studies were performed in 0.9% NaCl solution at 37 °C, and the obtained results were compared with ultrapure Ti (99.99%). The different phases of the microstructure were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Chemical composition and chemistry of the corroded surfaces were studied using X-ray photoelectron spectroscopy (XPS) analysis. For all studied alloys, the microstructure consisted of α matrix, which was strengthened by β phase. The highest and the lowest values of the β phase’s volume fraction were recorded for TC21 and Ti-Al-Nb alloys, respectively. The susceptibility of the investigated alloys toward pitting corrosion was enhanced following the sequence: Ti-6Al-7Nb < Ti-6Al-4V << TC21. Ti-6Al-7Nb alloy recorded the lowest pitting corrosion resistance (Rpit) among studied alloys, approaching that of pure Ti. The obvious changes in the microstructure of these alloys, together with XPS findings, were adopted to interpret the pronounced variation in the corrosion behavior of these materials.
Global Stability Analysis of Two-Stage Quarantine-Isolation Model with Holling Type II Incidence Function
Authors: Safi Mohammad A.
Published: April 2019

A new two-stage model for assessing the effect of basic control measures, quarantine and isolation, on a general disease transmission dynamic in a population is designed and rigorously analyzed. The model uses the Holling II incidence function for the infection rate. First, the basic reproduction number (

R
0

) is determined. The model has both locally and globally asymptotically stable disease-free equilibrium whenever

R
0

<
1

. If

R
0

>
1
,

then the disease is shown to be uniformly persistent. The model has a unique endemic equilibrium when

R
0

>
1
.

A nonlinear Lyapunov function is used in conjunction with LaSalle Invariance Principle to show that the endemic equilibrium is globally asymptotically stable for a special case.
Some Liouville Theorems on Finsler Manifolds
Authors: Wang Minqiu , Yin Songting
Published: April 2019

We give some Liouville type theorems of L p harmonic (resp. subharmonic, superharmonic) functions on a complete noncompact Finsler manifold. Using the geometric relationship between a Finsler metric and its reverse metric, we remove some restrictions on the reversibility. These improve the recent literature (Zhang and Xia, 2014).
Exploring the Antiangiogenic Potential of Solomonamide A Bioactive Precursors: In Vitro and In Vivo Evidences of the Inhibitory Activity of Solo F-OH During Angiogenesis
Authors: Carrillo Paloma , Martínez-Poveda Beatriz , Cheng-Sánchez Iván , Guerra Jessica , Tobia Chiara , López-Romero J. Manuel , Sarabia Francisco , Medina Miguel Ángel , Quesada Ana R.
Published: April 2019

Marine sponges are a prolific source of bioactive compounds. In this work, the putative antiangiogenic potential of a series of synthetic precursors of Solomonamide A, a cyclic peptide isolated from a marine sponge, was evaluated. By means of an in vitro screening, based on the inhibitory activity of endothelial tube formation, the compound Solo F–OH was selected for a deeper characterization of its antiangiogenic potential. Our results indicate that Solo F–OH is able to inhibit some key steps of the angiogenic process, including the proliferation, migration, and invasion of endothelial cells, as well as diminish their capability to degrade the extracellular matrix proteins. The antiangiogenic potential of Solo F–OH was confirmed by means of two different in vivo models: the chorioallantoic membrane (CAM) and the zebrafish yolk membrane (ZFYM) assays. The reduction in ERK1/2 and Akt phosphorylation in endothelial cells treated with Solo F–OH denotes that this compound could target the upstream components that are common to both pathways. Taken together, our results show a new and interesting biological activity of Solo F–OH as an inhibitor of the persistent and deregulated angiogenesis that characterizes cancer and other pathologies.
Microstructure and Magnetic Properties of Mn55Bi45 Powders Obtained by Different Ball Milling Processes
Authors: Li Xiang , Pan Dong , Xiang Zhen , Lu Wei , Batalu Dan
Published: April 2019

Low-temperature phase (LTP) MnBi is considered as a promising rare-earth-free permanent magnetic material with high coercivity and unique positive temperature coefficient of coercivity. Mn55Bi45 ribbons with high purity of LTP MnBi phase were prepared by melt spinning. Then, Mn55Bi45 powders with different particle size were obtained by low-energy ball milling (LEBM) with and without added surfactant. The coercivity is enhanced in both cases. Microstructure characterization reveals that Mn55Bi45 powders obtained by surfactant assisted low-energy ball milling (SALEBM) have better particle size uniformity and show higher decomposition of LTP MnBi. Coercivity can achieve a value of 17.2 kOe and the saturation magnetization (Ms) is 16 emu/g when Mn55Bi45 powders milled about 10 h by SALEBM. Coercivity has achieved a maximum value of 18.2 kOe at room temperature, and 23.5 kOe at 380 K after 14 h of LEBM. Furthermore, Mn55Bi45 powders obtained by LEBM have better magnetic properties.
Relevance of In Vitro Metabolism Models to PET Radiotracer Development: Prediction of In Vivo Clearance in Rats from Microsomal Stability Data
Authors: Schneider Daniela , Oskamp Angela , Holschbach Marcus , Neumaier Bernd , Bauer Andreas , Bier Dirk
Published: April 2019

The prediction of in vivo clearance from in vitro metabolism models such as liver microsomes is an established procedure in drug discovery. The potentials and limitations of this approach have been extensively evaluated in the pharmaceutical sector; however, this is not the case for the field of positron emission tomography (PET) radiotracer development. The application of PET radiotracers and classical drugs differs greatly with regard to the amount of substance administered. In typical PET imaging sessions, subnanomolar quantities of the radiotracer are injected, resulting in body concentrations that cannot be readily simulated in analytical assays. This raises concerns regarding the predictability of radiotracer clearance from in vitro data. We assessed the accuracy of clearance prediction for three prototypical PET radiotracers developed for imaging the A1 adenosine receptor (A1AR). Using the half-life (t1/2) approach and physiologically based scaling, in vivo clearance in the rat model was predicted from microsomal stability data. Actual clearance could be accurately predicted with an average fold error (AFE) of 0.78 and a root mean square error (RMSE) of 1.6. The observed slight underprediction (1.3-fold) is in accordance with the prediction accuracy reported for classical drugs. This result indicates that the prediction of radiotracer clearance is possible despite concentration differences of more than three orders of magnitude between in vitro and in vivo conditions. Consequently, in vitro metabolism models represent a valuable tool for PET radiotracer development.
Computational Methods Enabling Next-Generation Bioprocesses
Authors: Banga Julio R. , Menolascina Filippo
Published: April 2019

Synthetic biology—the engineering of cells to rewire the biomolecular networks inside them—has witnessed phenomenal progress [...]
Deity Citadels: Sacred Sites of Bio-Cultural Resistance and Resilience in Bhutan
Authors: Allison Elizabeth
Published: April 2019

Consistent with the pan-Himalayan tendency to see the landscape as lively and animated, protector deities and local spirits are perceived to inhabit various features of the landscape in Bhutan, causing these places to be treated with reverence and respect. Local spiritual beliefs are prized as central to the cultural identity of the Kingdom, making their way into government planning documents, town planning negotiations, and the 2008 Constitution. This elevation of local spiritual belief has been central to the maintenance and preservation of Bhutanese culture in its encounter with globally hegemonic social, economic, and political norms. Spirits and deities are believed to be the original owners of the land predating the introduction of Buddhism from Tibet. According to terma texts—spiritual treasures hidden by great Buddhist teachers to be discovered later—the initial introduction of Buddhism into Bhutan occurred in the seventh century. At that time, the Tibetan king Songtsen Gampo, the 32nd king of the Yarlung dynasty, built two temples in western and central parts of Bhutan as part of a strategy to pin down a demoness who was ravaging the Himalaya. About a century after the construction of the temples, Padmasambhava, known throughout the Himalayas as Guru Rimpoche, or “Precious Teacher,” arrived in Bhutan, subjugated eight classes of local spirits and made them sworn protectors of the Dharma. In this way, local deities and spirits became incorporated into Bhutan’s Vajrayana Buddhism to the extent that images of them are found at Buddhist temples and monasteries. Vajrayana Buddhism and local deities and spirits twine together in Bhutan to shape a cosmology that recognizes a spectrum of sentient beings, only some of whom are visible. The presence of deities and spirits informs local land use. Deity abodes or “citadels” (Dz.: pho brang) are restricted from human use. The presence of a deity citadel is sufficient in some locales to cause the diversion or reconsideration of human construction and resource use. By grounding spiritual beliefs in specific sites of the landscape, the citadels of deities sanctify the landscape, becoming nodes of resistance and resilience that support the Bhutanese in inhabiting their own internally-consistent cosmology, even as the pressures of global integration seek to impose hegemonic Western norms.
On the Design of a Safe Human-Friendly Teleoperated System for Doppler Sonography
Authors: Sandoval Arévalo Juan Sebastián , Laribi Med Amine , Zeghloul Saïd , Arsicault Marc
Published: April 2019

Variable stiffness actuators are employed to improve the safety features of robots that share a common workspace with humans. In this paper, a study of a joint variable stiffness device developed by PPRIME Institute—called V2SOM— for implementation in the joints of a multi-DoF robot is presented. A comparison of the interaction forces produced by a rigid body robot and a flexible robot using the V2SOM is provided through a dynamic simulator of a 7-DoF robot. As an example of potential applications, robot-assisted Doppler echography is proposed, which mainly focuses on guaranteeing patient safety when the robot holding the ultrasound probe comes into contact with the patient. For this purpose, an evaluation of both joint and Cartesian control approaches is provided. The simulation results allow us to corroborate the effectiveness of the V2SOM device to guarantee human safety when it is implemented in a multi-DoF robot.
Ionospheric Rayleigh Wave Disturbances Following the 2018 Alaska Earthquake from GPS Observations
Authors: Liu Yuhan , Jin Shuanggen
Published: April 2019

Big earthquakes often excite the acoustic resonance between the earth’s surface and the lower atmosphere. The perturbations can propagate upward into the ionosphere and trigger ionospheric anomalies detected by dual-frequency GPS observations, but coseismic ionospheric disturbance (CID) directivity and mechanism are not clear. In this paper, the ionospheric response to the Mw = 7.9 Alaska earthquake on 23 January 2018 is investigated from about 100 continuous GPS stations near the epicenter. The fourth-order zero-phase Butterworth band-pass filter with cutoffs of 2.2 mHz and 8 mHz is applied to obtain the ionospheric disturbances. Results show that the CIDs with an amplitude of up to 0.06 total electron content units (TECU) are detected about 10 min after the Alaska earthquake. The CIDs are as a result of the upward propagation acoustic waves triggered by the Rayleigh wave. The propagation velocities of TEC disturbances are around 2.6 km/s, which agree well with the wave propagation speed of 2.7 km/s detected by the bottom pressure records. Furthermore, the ionospheric disturbances following the 2018 Mw = 7.9 Alaska earthquake are inhomogeneous and directional which is rarely discussed. The magnitude of ionospheric disturbances in the western part of the epicenter is more obvious than in the eastern part. This phenomenon also corresponds to the data obtained from the seismographs and bottom pressure records (BPRs) at the eastern and western side of the epicenter.
Monitoring Extreme Agricultural Drought over the Horn of Africa (HOA) Using Remote Sensing Measurements
Authors: Qu Carolyn , Hao Xianjun , Qu John J.
Published: April 2019

The Horn of Africa ((HOA), including Djibouti, Eritrea, Ethiopia, and Somalia) has been slammed by extreme drought within the past years, and has become one of the most food-insecure regions in the world. Millions of people in the HOA are undernourished and are at risk of famine. Meanwhile, global climate change continues to cause more extreme weather and climate events, such as drought and heat waves, which have significant impacts on crop production and food security. This study aimed to investigate extreme drought in the Horn of Africa region, using satellite remote sensing data products from the Moderate Resolution Imaging Spectroradiometer (MODIS), a key instrument onboard the National Aeronautics and Space Administration (NASA) satellites Terra and Aqua, as well as Tropical Rainfall Measuring Mission (TRMM) precipitation data products. Normalized Difference Vegetation Index (NDVI), Vegetation Condition Index (VCI), Temperature Condition Index (TCI), and Vegetation Health Index (VHI) data from 2000 to 2017 were derived from the MODIS measurements and analyzed for assessments of the temporal trend of vegetation health and the impacts of extreme drought events. The results demonstrated the severity of vegetation stress and extreme drought during the past decades. From 1998 to 2017, monthly precipitation over major crop growth seasons decreased significantly. From 2001 to 2017, the mean VHI anomaly of HOA cropland decreased significantly, at a trend of −0.2364 ± 0.1446/year, and the mean TCI anomaly decreased at a trend of −0.2315 ± 0.2009/year. This indicated a deterioration of cropland due to drought conditions in the HOA. During most of the crop growth seasons in 2015 and 2016, the VHI values were below the 10-year (2001–2010) average: This was caused by extreme drought during the 2015–2016 El Niño event, one of the strongest El Niño events in recorded history. In addition, monthly VHI anomalies demonstrated a high correlation with monthly rainfall anomalies in July and August (the growth season of major crops in the HOA), and the trough points of the monthly rainfall and VHI anomaly time series of July and August were consistent with the timing of drought events and El Niño events.
Assessment of Individual Tree Detection and Canopy Cover Estimation using Unmanned Aerial Vehicle based Light Detection and Ranging (UAV-LiDAR) Data in Planted Forests
Authors: Wu Xiangqian , Shen Xin , Cao Lin , Wang Guibin , Cao Fuliang
Published: April 2019

Canopy cover is a key forest structural parameter that is commonly used in forest inventory, sustainable forest management and maintaining ecosystem services. Recently, much attention has been paid to the use of unmanned aerial vehicle (UAV)-based light detection and ranging (LiDAR) due to the flexibility, convenience, and high point density advantages of this method. In this study, we used UAV-based LiDAR data with individual tree segmentation-based method (ITSM), canopy height model-based method (CHMM), and a statistical model method (SMM) with LiDAR metrics to estimate the canopy cover of a pure ginkgo (Ginkgo biloba L.) planted forest in China. First, each individual tree within the plot was segmented using watershed, polynomial fitting, individual tree crown segmentation (ITCS) and point cloud segmentation (PCS) algorithms, and the canopy cover was calculated using the segmented individual tree crown (ITSM). Second, the CHM-based method, which was based on the CHM height threshold, was used to estimate the canopy cover in each plot. Third, the canopy cover was estimated using the multiple linear regression (MLR) model and assessed by leave-one-out cross validation. Finally, the performance of three canopy cover estimation methods was evaluated and compared by the canopy cover from the field data. The results demonstrated that, the PCS algorithm had the highest accuracy (F = 0.83), followed by the ITCS (F = 0.82) and watershed (F = 0.79) algorithms; the polynomial fitting algorithm had the lowest accuracy (F = 0.77). In the sensitivity analysis, the three CHM-based algorithms (i.e., watershed, polynomial fitting and ITCS) had the highest accuracy when the CHM resolution was 0.5 m, and the PCS algorithm had the highest accuracy when the distance threshold was 2 m. In addition, the ITSM had the highest accuracy in estimation of canopy cover (R2 = 0.92, rRMSE = 3.5%), followed by the CHMM (R2 = 0.94, rRMSE = 5.4%), and the SMM had a relative low accuracy (R2 = 0.80, rRMSE = 5.9%).The UAV-based LiDAR data can be effectively used in individual tree crown segmentation and canopy cover estimation at plot-level, and CC estimation methods can provide references for forest inventory, sustainable management and ecosystem assessment.
Hyperspectral Unmixing with Gaussian Mixture Model and Low-Rank Representation
Authors: Ma Yong , Jin Qiwen , Mei Xiaoguang , Dai Xiaobing , Fan Fan , Li Hao , Huang Jun
Published: April 2019

Gaussian mixture model (GMM) has been one of the most representative models for hyperspectral unmixing while considering endmember variability. However, the GMM unmixing models only have proper smoothness and sparsity prior constraints on the abundances and thus do not take into account the possible local spatial correlation. When the pixels that lie on the boundaries of different materials or the inhomogeneous region, the abundances of the neighboring pixels do not have those prior constraints. Thus, we propose a novel GMM unmixing method based on superpixel segmentation (SS) and low-rank representation (LRR), which is called GMM-SS-LRR. we adopt the SS in the first principal component of HSI to get the homogeneous regions. Moreover, the HSI to be unmixed is partitioned into regions where the statistical property of the abundance coefficients have the underlying low-rank property. Then, to further exploit the spatial data structure, under the Bayesian framework, we use GMM to formulate the unmixing problem, and put the low-rank property into the objective function as a prior knowledge, using generalized expectation maximization to solve the objection function. Experiments on synthetic datasets and real HSIs demonstrated that the proposed GMM-SS-LRR is efficient compared with other current popular methods.
A Framework for Estimating Clear-Sky Atmospheric Total Precipitable Water (TPW) from VIIRS/S-NPP
Authors: Zhou Shugui , Cheng Jie
Published: April 2019

Atmospheric water vapor content or total precipitable water (TPW) is a highly variable atmospheric constituent, yet it remains one of the meteorological parameters that is most difficult to characterize accurately. We develop a framework for estimating atmospheric TPW from Visible Infrared Imaging Radiometer Suite (VIIRS) data in this study. First, TPW is retrieved from VIIRS top-of-atmosphere (TOA) radiance of channels 15 and 16 using the refined split-window covariance-variance ratio (SWCVR) method. Then, the VIIRS TPW is blended with the microwave integrated retrieval system (MIRS) derived TPW via Bayesian model averaging (BMA) to improve the accuracy of VIIRS TPW. Three years (2014–2017) of ground measurements collected from SuomiNet sites over North America are used to validate the VIIRS TPW and blended TPW. The mean bias error (MBE) and root mean square error (RMSE) of the VIIRS TPW are 0.21 g/cm2 and 0.73 g/cm2, respectively, and the accuracy of the VIIRS TPW in daytime is much better than at night time. The MBE and RMSE of BMA integrated TPW are 0.06 g/cm2 and 0.35 g/cm2, and the accuracy difference between daytime and nighttime is also removed. The global radiosonde measurements are also collected to validate the BMA integrated VIIRS TPW. The MBE and RMSE of the BMA integrated TPW are 0.09 g/cm2 and 0.44 g/cm2 compared to the radiosonde measurements. This accuracy is also superior to the VIIRS TPW. Therefore, it is concluded that the developed framework can be used to derive accurate clear-sky TPW for VIIRS. This is the first time that we can obtain high accuracy TPW from VIIRS. This study will certainly benefit the study of atmospheric processes and climate change.
A Method for Simultaneously Measuring 6DOF Geometric Motion Errors of Linear and Rotary Axes Using Lasers
Authors: Zheng Fajia , Feng Qibo , Zhang Bin , Li Jiakun
Published: April 2019

A novel method for simultaneously directly measuring six-degrees-of-freedom (6DOF) geometric motion errors of CNC machine tools was proposed, and a corresponding measurement system was developed. This method can not only be applied for measuring a linear axis, but also for a rotary axis. A single-mode fiber was used to separate the measuring unit from the laser source in order to ensure system thermal stability and measurement accuracy. The method has the advantages of high efficiency and good accuracy, and requires no complicated decoupling calculation. The positioning error of the linear axis and radial motion error of the rotary axis are measured by laser interferometry and other 5DOF geometric motion errors by laser collimation. A series of experiments were performed to verify the feasibility and effectiveness of the developed measurement system.
Combined Thickness and Permittivity Measurement of Thin Layers with Open-Ended Coaxial Probes
Authors: Folgerø Kjetil , Haukalid Kjetil , Kocbach Jan , Peterson Andreas Soto
Published: April 2019

This paper presents a method to simultaneously determine the thickness and permittivity of thin layers from multi-frequency reflection coefficient measurements using an open-ended coaxial probe. This is achieved by exploiting that the probe becomes radiating at frequencies higher than the probe’s typical operating range. Permittivity information is extracted from measurements in the typical frequency range, whereas thickness information is obtained from high frequency measurements by exploiting resonances that occur when the radiated waves are reflected at the layer boundary. A finite element model of the measurement set-up is made in COMSOL MultiphysicsTM, and a matrix of simulations spanning the relevant layer thicknesses and permittivity range is generated. The measured permittivity spectra of unknown samples are compared to the simulation matrix to estimate layer thickness and permittivity. The method is verified by measurements of water–ethanol mixtures. An application example where the water fraction and layer thickness of a gas hydrate deposition layer is estimated from permittivity measurements in a multiphase flow loop is also presented.