bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Locus Phylogeny and Morphology Reveal Two New Species of Hypoxylon (Hypoxylaceae, Xylariales) from Motuo, China
Authors: Zhu An-Hong , Song Zi-Kun , Wang Jun-Fang , Guan Hao-Wen , Ma Hai-Xia
Published: December 2023

Hypoxylaceous fungi are abundant in China, but their discovery and report are uneven in various provinces, with more fungi in Yunnan and Hainan and fewer fungi in Tibet. During the investigation of macro-fungi in Motuo county, Tibet Autonomous Region, we collected a number of xylarialean specimens. Six hypoxylaceous specimens growing on dead angiosperm were collected from the forests of Motuo county, and they were described and illustrated as two new species in Hypoxylon based on a combination of morphological characters and molecular evidence. Hypoxylon diperithecium was characterized by its bistratal perithecia, purple-brown stromatal granules, citrine to rust KOH-extractable pigments, and light brown to brown ascospores ellipsoid-inequilateral with conspicuous coil-like ornamentation. Hypoxylon tibeticum was distinct from other species by having pulvinate and applanate stromata, surface vinaceous, with orange granules, orange KOH-extractable pigments, and brown ascospores with inconspicuous ornamentation. The multi-gene phylogenetic analyses (ITS-LSU-RPB2-TUB) supported the two new taxa as separate lineages in the genus Hypoxylon. A key to all known Hypoxylon taxa from China is provided.
Shaping the Future of Probiotics: Novel Methodologies, Applications, and Mechanisms of Action
Authors: Galanis Alex
Published: December 2023

Probiotics are defined as live microorganisms that, when consumed in appropriate amounts, can promote host homeostasis, and induce health-promoting effects [...]
Recovery of Demolished House Rocks from Construction and Demolition Waste with Water Jigs
Authors: Teixeira Artur Bressanelli , Barkat Hassan , Sampaio Carlos Hoffmann , Moncunill Josep Oliva
Published: December 2023

The European Union (EU) is responsible for generating quantities ranging from 310 to almost 700 million tons of construction and demolition waste (CDW) per year. Consisting of over 70% inert material (concrete, ceramics, plaster, bricks, and rocks), CDW can be recycled for various uses, and studies on the concentration of the materials of interest are necessary to improve the management of this material and reduce waste. In CDW recycling plants in Spain, there is a significant presence of limestone from old houses (a common material used in civil construction before new construction materials and technologies emerged) that were demolished and mixed with CDW that can be recovered for use as aggregates in concretes with process density concentration processes such as water jigging. The jigging process is based on the difference in density between materials, allowing the concentration of the densest material at the bottom of the jig. Concrete, conventional construction bricks, and rocks from old houses were taken separately and then were crushed and mixed based on binary and ternary tests, and each test was performed in this study by applying the jigging separation method. The physical characterization tests of these materials was carried out to observe the jigging performance in the concentration of rocks as well as the aggregates present in concrete. Binary tests (with two different materials) and ternary tests (with three different materials) were carried out to analyze the concentration of particles with a density greater than 2.55 g/cm3. The efficiency of jigging in the concentration of these materials was proven, and products were generated with more than 70% recovery of this material, with a concentration comprised of more than 95% rocks and concrete.
Field Simulation Technique to Enhance the Mechanical Strength and Elemental Composition of Soft Clay Soil Using Thermal Treatment
Authors: Shareef Ali H. , Al-Neami Mohammed A. , Rahil Falah H.
Published: December 2023

This paper aims to improve the strength of soft clay soil using thermal treatment through a laboratory study that simulates the field application. The laboratory work consisted of preparing a soft clay (Cu = 14 kPa) inside a metal box (92.5 × 92.5 × 92.5) cm3. Boreholes of diameter 43 mm, with different lengths, spacing, and arrangements, were made inside the soil to work as a guide for heating pipes which connected to a controlled heating system. A novel heating system, using gas as a heat source, was developed and manufactured. After the end of the treatment periods, a load was applied until failure on a (20 × 20) cm2 square footing. Various parameter spacings (3, 4, and 5 times the outer diameter of the borehole), depths (1, 1.5, 2, and 2.5 times the width of the model footing), arrangements (square, circular, and triangular), and heating periods (2, 4, 6, 8, and 10 h) were investigated. The results showed the strength and behavior of the soil when subjected to the heated boreholes at different spacings, depths, and heating times, which were determined to be three times the outer diameter of the borehole, two times the width of the square footing, and eight hours, respectively, while the effect of the arrangement of the heated borehole casings was small. Also, a cone penetration probe (CPT) conducted on the heated soil showed that the unconsolidated shear strength (Cu) increased from 14 to 360 kPa and then decreased to 140 kPa (as an average with depth). In contrast, the average angle of internal friction (Ø) increased from 0 to 52 degrees and decreased to 16 degrees (as an average with depth) from the center of the heating model to the furthest point affected by heating. The EDS formula showed that components such as silicon, aluminum, and iron decreased at 300 °C and increased at 400 °C in the treated soils. The calcium content increased at 200 °C and then decreased sharply at 400 °C. The carbon percentage increased at 300 °C and decreased at 400 °C. The elemental proportions showed little change or remained stable at temperatures between 400 °C and 600 °C.
Identification of Some Gem Quality Blue to Green Li-Tourmalines
Authors: Pasetti Lorenzo , Borromeo Laura , Bersani Danilo , Andò Sergio , Schnellrath Jurgen , Hennebois Ugo , Karampelas Stefanos
Published: December 2023

Due to their appealing colors, gem quality tourmalines, particularly the blue to green Cu- and Mn-bearing Li-tourmalines known as the Paraíba type, have been of significant interest since their discovery at the end of 1980s. At the same time, the demand of other similar colored tourmalines increased. Most Paraíba-type tourmalines belong to the elbaite species; however, liddicoatite gems can also be found. Recognizing and classifying various tourmaline species, especially these valued Paraíba-type tourmalines, are important for geologists, mineralogists, mineral collectors, and gemologists. This study explores the application of Raman spectroscopy in random crystal orientations to distinguish between the elbaite and liddicoatite tourmaline species. Raman spectra were collected from faceted blue to green Li-tourmalines alongside chemical analysis using EDXRF (Energy Dispersive X-ray Fluorescence), UV-Vis-NIR (Ultraviolet-Visible-Near InfraRed Spectroscopy), and PL (Photoluminescence spectroscopy) to provide comprehensive characterization. The results show that Raman spectroscopy, particularly in the OH stretching region, is a useful tool for differentiating elbaite from liddicoatite, and this identification remains consistent regardless of crystal orientation. The fingerprint region in the Raman spectra, on the other hand, is orientation-dependent and can only differentiate the two species when detected in specific orientations. Furthermore, Paraíba-type tourmalines can be identified by visible-near infrared (Vis-NIR) spectroscopy, although not by Raman spectroscopy.
Effect of Film Morphology on Electrical Conductivity of PEDOT:PSS
Authors: Saha Aditya , Ohori Daisuke , Sasaki Takahiko , Itoh Keisuke , Oshima Ryuji , Samukawa Seiji
Published: December 2023

Commercially available formulations of the popular conductive polymer, poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) are aqueous dispersions that require the addition of secondary dopants such as dimethyl sulphoxide (DMSO) or ethylene glycol (EG) for fabricated films to have the desired levels of conductivity. CleviosTM F HC Solar, a formulation of PEDOT:PSS produced by Heraeus, GmbH, achieves over 500 S/cm without these secondary dopants. This work studies whether secondary dopants such as DMSO have any additional effect on this type of PEDOT:PSS. The temperature dependencies of the conductivity of F HC Solar spin-coated thin films measured using a four-probe method seem to exhibit different charge transport properties compared with secondary doped PH1000. Observations made using atomic force microscopy (AFM) show that different concentrations of DMSO affect the orientation of the PEDOT domains in the thin film. These morphological changes cause room temperature conductivity to reduce from 640 S/cm in pristine films to as low as 555 S/cm after adding 7 wt% of DMSO along the film. Such tuning may prove useful in future applications of PEDOT:PSS, such as nanoprobes, transistors and hybrid solar cells.
Characterization and Exploration of the Neuroprotective Potential of Oat-Protein-Derived Peptides in PC12 Cells and Scopolamine-Treated Zebrafish
Authors: Rafique Hamad , Hu Xinzhong , Ren Tian , Dong Rui , Aadil Rana Muhammad , Zou Liang , Sharif Mian Kamran , Li Lu
Published: December 2023

Neurodegenerative disorders pose a substantial risk to human health, and oxidative stress, cholinergic dysfunction, and inflammation are the major contributors. The purpose of this study was to explore the neuroprotective effects of oat protein hydrolysate (OPH) and identify peptides with neuroprotective potential. This study is the first to isolate and identify OPH peptides with neuroprotective potential, including DFVADHPFLF (DF-10), HGQNFPIL (HL-8), and RDFPITWPW (RW-9), by screening via peptidomes and molecular-docking simulations. These peptides showed positive effects on the activity of antioxidant enzymes and thus reduced oxidative stress through regulation of Nrf2-keap1/HO-1 gene expression in vitro and in vivo. The peptides also significantly ameliorated scopolamine-induced cognitive impairment in the zebrafish model. This improvement was correlated with mitigation of MDA levels, AChE activity, and levels of inflammatory cytokines in the brains of zebrafish. Furthermore, these peptides significantly upregulated the mRNA expression of Bdnf, Nrf2, and Erg1 in the brains of zebrafish with neurodegenerative disorders. Collectively, oat peptides have potential for use as active components in nutraceutical applications for the prevention of neurodegenerative diseases.
PsyNBIOsis: Investigating the Association between Maternal Gestational Diabetes, Mental Health, Diet and Childhood Obesity Risk: Protocol for a Prospective, Longitudinal, Observational Study
Authors: Gilbert Leah , Raubenheimer David , Hibbert Emily J. , Nanan Ralph
Published: December 2023

Background: Gestational diabetes mellitus (GDM) is associated with poorer maternal mental health (depression and anxiety). Maternal mental health and GDM are likely to influence diet, which in turn impacts the course of GDM. Maternal diet may also be directly or indirectly associated with changes in infant anthropometry. The aims of this study are to (1) examine the associations between maternal GDM, mental health and diet, and (2) evaluate the associations between these maternal factors, breastmilk composition and infant anthropometry. Methods: This prospective, observational, longitudinal cohort study compares a cohort of women with and without GDM. Maternal mental health and diet are assessed using validated questionnaires. Breastmilk composition is measured with the Human Milk Analyzer, and infant body composition is measured with air displacement plethysmography. Significance and Impact: Once data have been collected, PsyNBIOsis will provide evidence for the associations between maternal mental health, GDM status and diet, and their impact on breastmilk composition and early infant growth. The results may inform the Developmental Origins of Health and Disease framework and provide data on which to build cost-effective interventions to prevent both the development of mental health issues in mothers and adverse growth patterns in infants.
Association between Western Dietary Patterns, Typical Food Groups, and Behavioral Health Disorders: An Updated Systematic Review and Meta-Analysis of Observational Studies
Authors: Zhang Huang , Li Maiquan , Mo Lan , Luo Jie , Shen Qingwu , Quan Wei
Published: December 2023

Western dietary patterns (WDP) and typical food groups may play a major role in the risk of behavioral health disorders. Nevertheless, the relationships between WDP, common food categories, and mental health disorders lack consistency and remain incompletely understood in relation to potential mechanisms. Therefore, the objective of the present study was conducted to synthesize available evidence linking WDP and typical food groups to these outcomes. Web of Science, PubMed, EMBASE, and MEDLINE were searched up to August 2023. Random effect meta-analyses were performed to obtain pooled odds ratio and the relative risk for the prevalence of outcomes and the incidence of outcomes, respectively. A total of 54 articles were included. WDP was associated with increased risk of both depression (1.19; 95% CI: 1.06–1.32) and depressive symptoms (1.20; 95% CI: 1.08–1.34). Except for high-fat dairy products, food groups are associated with an increased risk of anxiety, depression, and depressive symptoms. This review presents evidence to further understand the relationship between WDP, typical food groups, and the incidence of behavioral health disorders, and more randomized controlled trials and cohort studies are urgently required to confirm these findings and elucidate potential mechanisms.
First Identification of Trichinella pseudospiralis in a Golden Jackal (Canis aureus) in Romania
Authors: Marin Ana-Maria , Popovici Dan-Cornel , Marucci Gianluca , Cherchi Simona , Mederle Narcisa
Published: December 2023

Trichinella spp. are etiological zoonotic agents that spread throughout the world and affect mammals, birds, and reptiles. Within this genus, Trichinella pseudospiralis is the only recognized non-encapsulated species known to infect mammals and birds. This species has been reported in the majority of European countries, and the real epidemiological scenario of this species remains to be defined because its detection in mammals is much lower than that of the capsulated species. The aim of this study was to examine the presence of Trichinella larvae isolated from the muscles of a jackal from the hunting fund of 36 Murfatlar, Constanta County, Romania. The muscle samples were examined by artificial digestion, and the larvae were identified at the species level by multiplex PCR. The presence of larvae belonging to T. pseudospiralis, a species more frequently reported in carnivorous birds, was observed. This study describes the first identification of T. pseudospiralis in a jackal. The results suggest that there is an urgent need to investigate which species of mammals and/or birds act as reservoirs for this zoonotic nematode in Romania.
Pathogenic Potential and Antibiotic Susceptibility: A Comprehensive Study of Enterococci from Different Ecological Settings
Authors: Pandova Maria , Kizheva Yoana , Tsenova Margarita , Rusinova Mariya , Borisova Tsvetomira , Hristova Petya
Published: December 2023

The pathway and the lifestyle of known enterococcus species are too complicated. The aim of the present study is to trace the path of pathogenicity of enterococci isolated from seven habitats (Cornu aspersum intestine; Bulgarian yoghurt; goat and cow feta cheese—mature and young, respectively; Arabian street food—doner kebab; cow milk; and human breast milk) by comparing their pathogenic potential. In total, 72 enterococcal strains were isolated and identified by MALDI-TOF, sequencing, and PCR. Hemolytic and gelatinase activity were biochemically determined. PCR was carried out for detection of virulence factors (cylB, esp, gls24, nucl, psaA, agg, gelE, and ace) and antibiotic resistance (erm, ermB, blaZ, vanA, aphA, mefA, gyrA, catpIP501, and aac6′-aph2″). Phenotypic antibiotic resistance was assigned according to EUCAST. Eleven representatives of the genus Enterococcus were identified: E. mundtii, E. casseliflavus, E. gilvus, E. pseudoavium, E. pallens, E. malodoratus, E. devriesei, E. gallinarum, E. durans, E. faecium, and E. faecalis. Twenty-two strains expressed α-hemolysis. Thirteen strains had the cylB gene. Only two strains expressed α-hemolysis and possessed the cylB gene simultaneously. Positive amplification for gelE was found in 35% of the isolates, but phenotypic gelatinase activity was observed only in three strains. All isolates showed varying antibiotic resistance. Only E. faecalis BM15 showed multiple resistance (AMP-HLSR-RP). Correlation between genotypic and phenotypic macrolide resistance was revealed for two E. faecalis strains.
The Discovery of Novel Agents against Staphylococcus aureus by Targeting Sortase A: A Combination of Virtual Screening and Experimental Validation
Authors: Liu Kang , Tong Jiangbo , Liu Xu , Liang Dan , Ren Fangzhe , Jiang Nan , Hao Zhenyu , Li Shixin , Wang Qiang
Published: December 2023

Staphylococcus aureus (S. aureus), commonly known as “superbugs”, is a highly pathogenic bacterium that poses a serious threat to human health. There is an urgent need to replace traditional antibiotics with novel drugs to combat S. aureus. Sortase A (SrtA) is a crucial transpeptidase involved in the adhesion process of S. aureus. The reduction in virulence and prevention of S. aureus infections have made it a significant target for antimicrobial drugs. In this study, we combined virtual screening with experimental validation to identify potential drug candidates from a drug library. Three hits, referred to as Naldemedine, Telmisartan, and Azilsartan, were identified based on docking binding energy and the ratio of occupied functional sites of SrtA. The stability analysis manifests that Naldemedine and Telmisartan have a higher binding affinity to the hydrophobic pockets. Specifically, Telmisartan forms stable hydrogen bonds with SrtA, resulting in the highest binding energy. Our experiments prove that the efficiency of adhesion and invasion by S. aureus can be decreased without significantly affecting bacterial growth. Our work identifies Telmisartan as the most promising candidate for inhibiting SrtA, which can help combat S. aureus infection.
Assessment of Some Unsymmetrical Porphyrins as Promising Molecules for Photodynamic Therapy of Cutaneous Disorders
Authors: Burloiu Andreea Mihaela , Manda Gina , Lupuliasa Dumitru , Socoteanu Radu Petre , Mihai Dragos Paul , Neagoe Ionela Victoria , Anghelache Laurentiu-Iliuta , Surcel Mihaela , Anastasescu Mihai , Olariu Laura , Gîrd Cerasela Elena , Barbuceanu Stefania Felicia , Ferreira Luis Filipe Vieira , Boscencu Rica
Published: December 2023

In order to select for further development novel photosensitizers for photodynamic therapy in cutaneous disorders, three unsymmetrical porphyrins, namely 5-(4-hydroxy-3-methoxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl) porphyrin (P2.2), 5-(2-hydroxy-5-methoxyphenyl)-10,15,20-tris-(4-carboxymethylphenyl) porphyrin (P3.2), and 5-(2,4-dihydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl) porphyrin (P4.2), along with their fully symmetrical counterparts 5,10,15,20-tetrakis-(4-acetoxy-3-methoxyphenyl) porphyrin (P2.1) and 5,10,15,20-tetrakis-(4-carboxymethylphenyl) porphyrin (P3.1) were comparatively evaluated. The absorption and fluorescence properties, as well as atomic force microscopy measurements were performed to evaluate the photophysical characteristics as well as morphological and textural properties of the mentioned porphyrins. The cellular uptake of compounds and the effect of photodynamic therapy on the viability, proliferation, and necrosis of human HaCaT keratinocytes, human Hs27 skin fibroblasts, human skin SCL II squamous cell carcinoma, and B16F10 melanoma cells were assessed in vitro, in correlation with the structural and photophysical properties of the investigated porphyrins, and with the predictions regarding diffusion through cell membranes and ADMET properties. All samples were found to be isotropic and self-similar, with slightly different degrees of aggregability, had a relatively low predicted toxicity (class V), and a predicted long half-life after systemic administration. The in vitro study performed on non-malignant and malignant skin-relevant cells highlighted that the asymmetric P2.2 porphyrin qualified among the five investigated porphyrins to be a promising photosensitizer candidate for PDT in skin disorders. P2.2 was shown to accumulate well within cells, and induced by PDT a massive decrease in the number of metabolically active skin cells, partly due to cell death by necrosis. P2.2 had in this respect a better behavior than the symmetric P.2.1 compound and the related asymmetric compound P4.2. The strong action of P2.2-mediated PDT on normal skin cells might be an important drawback for further development of this compound. Meanwhile, the P3.1 and P3.2 compounds were not able to accumulate well in skin cells, and did not elicit significant PDT in vitro. Taken together, our experiments suggest that P2.2 can be a promising candidate for the development of novel photosensitizers for PDT in skin disorders.
Paclitaxel and Therapeutic Drug Monitoring with Microsampling in Clinical Practice
Authors: Radovanovic Mirjana , Galettis Peter , Flynn Alex , Martin Jennifer H. , Schneider Jennifer J.
Published: December 2023

Paclitaxel is an anticancer agent efficacious in various tumors. There is large interindividual variability in drug plasma concentrations resulting in a wide variability in observed toxicity in patients. Studies have shown the time the concentration of paclitaxel exceeds 0.05 µM is a predictive parameter of toxicity, making dose individualization potentially useful in reducing the adverse effects. To determine paclitaxel drug concentration, a venous blood sample collected 24 h following the end of infusion is required, often inconvenient for patients. Alternatively, using a microsampling device for self-sampling would facilitate paclitaxel monitoring regardless of the patient’s location. We investigated the feasibility of collecting venous and capillary samples (using a Mitra® device) from cancer patients to determine the paclitaxel concentrations. The relationship between the venous plasma and whole blood and venous and capillary blood (on Mitra®) paclitaxel concentrations, defined by a Passing–Bablok regression, were 0.8433 and 0.8569, respectively. Demonstrating a clinically acceptable relationship between plasma and whole blood paclitaxel concentration would reduce the need to establish new target concentrations in whole blood. However, in this study, comparison of venous and capillary blood using Mitra® for sampling displayed wide confidence intervals suggesting the results from the plasma and whole blood on this device may not be interchangeable.
Eugenol-Rich Essential Oil from Pimenta dioica: In Vitro and In Vivo Potentialities against Leishmania amazonensis
Authors: Monzote Lianet , Machín Laura , González Adiel , Scull Ramón , Gutiérrez Yamilet I. , Satyal Prabodh , Gille Lars , Setzer William N.
Published: December 2023

Pimenta dioica L. is one the most recognized species with diverse biological activities. In this study, in vitro activity and in vivo efficacy of essential oil from P. dioica (EO-Pd) was evaluated. The main compound was also included in the animal studies and its in silico prediction related to biological activities, molecular ligands, drug likeness, and ADME (absorption, distribution, metabolism, and excretion) properties are listed. The chemical composition analyzed by GC-MS retrieved 45 components, which the most abundant compound was the eugenol (80.1%). The EO-Pd was able to inhibit the growth of L. amazonensis (IC50 = 9.7 ± 0.7 and 11.3 ± 2.1 µg/mL, promastigotes and amastigotes, respectively). The cytotoxicity assay showed a CC50 of 104.5 ± 0.9 µg/mL and a selectivity index of 9. In the model of cutaneous leishmaniasis in BALB/c mice, the effect of EO-Pd and eugenol was observed after treatment at 30 mg/kg by intralesional route with 5 administrations every 4 days. In the in silico predictions, some targets that justified the antileishmanial activity of eugenol and good drug like properties for this compound, were obtained. This study showed for first time the potential of EO-Pd to inhibit L. amazonensis, which could be linked to the activity of major compound eugenol.
Systemic and Brain Pharmacokinetics of Milnacipran in Mice: Comparison of Intraperitoneal and Intravenous Administration
Authors: Bagchi Sounak , Nozohouri Ehsan , Ahn Yeseul , Patel Dhavalkumar , Bickel Ulrich , Karamyan Vardan T.
Published: December 2023

Milnacipran is a dual serotonin and norepinephrine reuptake inhibitor, clinically used for the treatment of major depression or fibromyalgia. Currently, there are no studies reporting the pharmacokinetics (PK) of milnacipran after intraperitoneal (IP) injection, despite this being the primary administration route in numerous experimental studies using the drug. Therefore, the present study was designed to investigate the PK profile of IP-administered milnacipran in mice and compare it to the intravenous (IV) route. First a liquid chromatography–mass spectrometry (LC-MS/MS) method was developed and validated to accurately quantify milnacipran in biological samples. The method was used to quantify milnacipran in blood and brain samples collected at various time-points post-administration. Non-compartmental and PK analyses were employed to determine key PK parameters. The maximum concentration (Cmax) of the drug in plasma was at 5 min after IP administration, whereas in the brain, it was at 60 min for both routes of administration. Curiously, the majority of PK parameters were similar irrespective of the administration route, and the bioavailability was 92.5% after the IP injection. These findings provide insight into milnacipran’s absorption, distribution, and elimination characteristics in mice after IP administration for the first time and should be valuable for future pharmacological studies.
Role of Histone Deacetylase 6 and Histone Deacetylase 6 Inhibition in Colorectal Cancer
Authors: Vuletić Ana , Mirjačić Martinović Katarina , Spasić Jelena
Published: December 2023

Histone deacetylase 6 (HDAC6), by deacetylation of multiple substrates and association with interacting proteins, regulates many physiological processes that are involved in cancer development and invasiveness such as cell proliferation, apoptosis, motility, epithelial to mesenchymal transition, and angiogenesis. Due to its ability to remove misfolded proteins, induce autophagy, and regulate unfolded protein response, HDAC6 plays a protective role in responses to stress and enables tumor cell survival. The scope of this review is to discuss the roles of HDCA6 and its implications for the therapy of colorectal cancer (CRC). As HDAC6 is overexpressed in CRC, correlates with poor disease prognosis, and is not essential for normal mammalian development, it represents a good therapeutic target. Selective inhibition of HDAC6 impairs growth and progression without inducing major adverse events in experimental animals. In CRC, HDAC6 inhibitors have shown the potential to reduce tumor progression and enhance the therapeutic effect of other drugs. As HDAC6 is involved in the regulation of immune responses, HDAC6 inhibitors have shown the potential to improve antitumor immunity by increasing the immunogenicity of tumor cells, augmenting immune cell activity, and alleviating immunosuppression in the tumor microenvironment. Therefore, HDAC6 inhibitors may represent promising candidates to improve the effect of and overcome resistance to immunotherapy.
Sensitizing the Efficiency of ICIs by Neoantigen mRNA Vaccines for HCC Treatment
Authors: Han Rui , Wang Yuqian , Lu Lingeng
Published: December 2023

This study builds upon the groundbreaking mRNA vaccine Nobel Prize win in 2023 for COVID-19 prevention, paving the way for next-generation mRNA cancer vaccines to revolutionize immunotherapy. Despite the existing challenges, such as the presence of a suppressive tumor microenvironment and the identification of cancer-associated antigens, recent results from the KEYNOTE-942 trial have successfully demonstrated the effectiveness of mRNA-based cancer treatments, providing clinical evidence for the first time. This trial aimed to evaluate the efficacy and safety of combining immune checkpoint inhibitors with mRNA-based therapies in treating cancer. This advancement undeniably represents new hope for hepatocellular carcinoma (HCC) patients. However, progress in this field remains limited. In this article, we summarized the current state of applying immune checkpoint inhibitors (ICIs) combined with neoantigen mRNA vaccines. Additionally, we discussed potential targets for designing novel mRNA vaccines and potential mRNA vaccine delivery vehicles. The objective of this article is to inspire enthusiasm for the exploration of innovative therapeutic strategies that combine ICIs with neoantigen mRNA vaccines for HCC treatment and HCC prevention.
Facile Strategy for Boosting of Inorganic Fillers Retention in Paper
Authors: Maślana Klaudia , Sielicki Krzysztof , Wenelska Karolina , Kędzierski Tomasz , Janusz Joanna , Mariańczyk Grzegorz , Gorgon-Kuza Aleksandra , Bogdan Wojciech , Zielińska Beata , Mijowska Ewa
Published: December 2023

Achieving the desired properties of paper such as strength, durability, and printability remains challenging. Paper mills employ calcium carbonate (CaCO3) as a filler to boost paper’s brightness, opacity, and printability. However, weak interaction between cellulose fibers and CaCO3 particles creates different issues in the papermaking industry. Therefore, this study explores the influence of various inorganic additives as crosslinkers such as mesoporous SiO2 nanospheres, TiO2 nanoparticles, h-BN nanoflakes, and hydroxylated h-BN nanoflakes (h-BN-OH) on inorganic fillers content in the paper. They were introduced to the paper pulp in the form of a polyethylene glycol (PEG) suspension to enable bonding between the inorganic particles and the paper pulp. Our findings have been revealed based on detailed microscopic and structural analyses, e.g., transmission and scanning electron microscopy, X-ray diffraction, Raman spectroscopy, and N2 adsorption/desorption isotherms. Finally, the inorganic fillers (CaCO3 and respective inorganic additives) content was evaluated following ISO 1762:2001 guidelines. Conducted evaluations allowed us to identify the most efficient crosslinker (SiO2 nanoparticles) in terms of inorganic filler retention. Paper sheets modified with SiO2 enhance the retention of the fillers by ~12.1%. Therefore, we believe these findings offer valuable insights for enhancing the papermaking process toward boosting the quality of the resulting paper.
Design of Experiment for Optimizing Microencapsulation by the Solvent Evaporation Technique
Authors: Loureiro Mónica V. , Aguiar António , Santos Rui G. dos , Bordado João C. , Pinho Isabel , Marques Ana C.
Published: December 2023

We employed microemulsion combined with the solvent evaporation technique to produce biodegradable polycaprolactone (PCL) MCs, containing encapsulated isophorone diisocyanate (IPDI), to act as crosslinkers in high-performance adhesive formulations. The MC production process was optimized by applying a design of experiment (DoE) statistical approach, aimed at decreasing the MCs’ average size. For that, three different factors were considered, namely the concentration of two emulsifiers, polyvinyl alcohol (PVA) and gum arabic (GA); and the oil-to-water phase ratio of the emulsion. The significance of each factor was evaluated, and a predictive model was developed. We were able to decrease the average MC size from 326 μm to 70 µm, maintaining a high encapsulation yield of approximately 60% of the MCs’ weight, and a very satisfactory shelf life. The MCs’ average size optimization enabled us to obtain an improved distributive and dispersive mixture of isocyanate-loaded MCs at the adhesive bond. The MCs’ suitability as crosslinkers for footwear adhesives was assessed following industry standards. Peel tests revealed peel strength values above the minimum required for casual footwear, while the creep test results indicated an effective crosslinking of the adhesive. These results confirm the ability of the MCs to release IPDI during the adhesion process and act as crosslinkers for new adhesive formulations.
High-Thermal Stable Epoxy Resin through Blending Nanoarchitectonics with Double-Decker-Shaped Polyhedral Silsesquioxane-Functionalized Benzoxazine Derivatives
Authors: Kao Yang-Chin , Lin Jing-Yu , Chen Wei-Cheng , Gamal Mohamed Mohamed , Huang Chih-Feng , Chen Jung-Hui , Kuo Shiao-Wei
Published: December 2023

A series of di-functional benzoxazine (BZ) monomers was synthesized, specifically the double-decker silsesquioxane (DDSQ) cage structure (DDSQ-BZ). Comparative analyses were conducted between DDSQ-BZ monomers and the most commonly utilized bisphenol A-functionalized bifunctional benzoxazine (BPA-BZ) monomer. DDSQ-BZ compounds possess better thermal properties such as high char yield and high thermal decomposition temperature (Td10) after thermal ring-opening polymerization (ROP) because the inorganic DDSQ cage nanostructure features a nano-reinforcement effect. In addition, blending inorganic DDSQ-BZ compounds with epoxy resin was explored to form organic/inorganic hybrids with enhanced thermal and mechanical properties following thermal ROP. The improvement in mechanical properties is primarily attributed to the network structure formed by the cross-linking between DDSQ-BZ and the epoxy resin during thermal ROP, as well as hydrogen bonding interactions formed between the hydroxyl groups generated during thermal ROP and the Si-O-Si bonds in the DDSQ structure.
Enhanced DC Dielectric Properties of Crosslinked Polyethylene Comprehensively Modified by the Grafting of a Multifunctional Voltage Stabilizer
Authors: Li Peng , Wang Xuan , Jin Jin , Sun Xiangxiang , Zhang Hui , Zhang Runsheng
Published: December 2023

In this paper, a new multifunctional compound, 1,1′-(oxalylbis(4,1-phenylene))bis(1H-pyrrole-2,5-dione) (BVM), is grafted onto crosslinked polyethylene (XLPE) by radical-initiated grafting to play triple roles as a voltage stabilizer, space-charge inhibitor and crosslinking auxiliary and to achieve the purpose of comprehensively enhancing the DC dielectric properties of polymers while decreasing the type and number of additives. By analyzing the DC breakdown field strength, current density and space-charge distribution of the materials at different temperatures, it is demonstrated that BVM grafting can comprehensively and effectively enhance the electrical properties of the materials, with little dependence on temperature. The BVM molecule has two polar groups and an effective molecular structure that acts as a voltage stabilizer, thus enabling the introduction of dense, uniform, deeply trapped energy levels within the material to inhibit the space charge and to capture high-energy electrons to prevent damage to the material structure; however, the two functions do not affect each other. This is also consistent with first-principles calculations and quantum-chemical calculations. Gel content testing shows no effect on polymer crosslinking, even with a 27.8% reduction in the amount of the crosslinking agent di-isopropyl peroxide (DCP), which reduces the damage to the polymer’s electrical resistance caused by the byproducts of DCP decomposition. Therefore, grafting multifunctional BVM compounds to improve the dielectric characteristics of polymers is a viable area of study in the development of high-voltage direct current (HVDC) cable materials.
Investigation of the Mechanism for Removal of Typical Pathogenic Bacteria from Three-Compartment Septic Tanks under Low Temperature Conditions
Authors: Cheng Shenwei , Yang Shuoxin , Huang Jianyin , Liu Fang , Shen Feng
Published: December 2023

Three-compartment septic tanks are a prominently advocated environmentally sustainable sanitation facility in rural China. However, the comprehensive elimination efficacy and underlying mechanisms of pathogenic bacteria within septic tanks remain incompletely understood. In particular, the operational performance in low-temperature conditions has received limited attention in the existing literature. In this work, a simulation of the three-compartment septic tank treatment system was conducted under low-temperature conditions (15 °C). The operational results exemplify the synergistic interplay of volatile fatty acids (VFAs), NH3-N, and bacterial communities, culminating in a partial reduction in Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis, within the three-compartment septic tank. Their respective population abundances were decreased by magnitudes of 2.2, 1.3, 0.03, and 1.46 logarithmic units (copies/mL), respectively. Through the utilization of qPCR and physicochemical indicators, it was observed that the bactericidal effect of VFA primarily occurred during the initial 0–21-day period, while NH3-N consistently proved to be the most vital sterilizing agent throughout the operation of the three-compartment septic tank. Predominant bacterial communities within the septic tank, such as Christensenellaceae_R-7_group, Brevundimonas, Acinetobacter, and Saccharimonadales, exerted substantial inhibitory impacts on Enterococcus faecalis, Escherichia coli, and Salmonella enteritidis through niche competition and suppression. In essence, this study elucidated the actual efficiency of elimination and the underlying mechanisms of typical pathogenic bacteria within three-compartment septic tanks in low-temperature conditions, thereby providing compelling evidence supporting the viability of environmentally sound treatment using such septic tanks. Concurrently, it also shed light on several limitations associated with this treatment approach, aiming to contribute valuable insights for the assessment of ecological risks and health hazards associated with the environmentally benign treatment of rural toilet waste.
Exploring Pathways toward the Development of High-Proportion Solar Photovoltaic Generation for Carbon Neutrality: The Example of China
Authors: Yuan Jiehui , Tang Xiaoming , Yuan Wenli
Published: December 2023

Solar photovoltaic (PV) generation will play a crucial role in the global clean energy transition toward carbon neutrality. While the development of solar PV generation has been explored in depth, the development of high-proportion solar PV generation has yet to be discussed. Considering the back force of the constraint of achieving carbon neutrality within the specified timeframe, this paper establishes a unified, multi-dimensional, and achievable framework through which to perform a system analysis for exploring the potential risks and challenges involved in the development process of high-proportion solar PV generation and investigating possible pathways to fostering the development of high-proportion solar PV generation. The results show that the critical risks and challenges include a low conversion efficiency, poorer resource endowment, more limited land resources, a low use of rooftop resources, an increasing complexity of power system scheduling, and low public awareness. These challenges have emerged with the development of solar PV generation in China and the aim of developing high-proportion solar PV generation. Based on our findings, possible pathways toward developing high-proportion solar PV generation have been determined, including promoting the research and development of higher PV efficiency, determining the optimal development sequence of solar resources, tapping the potential of land resources, increasing the use of rooftop resources, improving the resilience of the power system, and enhancing the public awareness of deploying solar PV generation. Finally, recommendations are proposed to optimize policy formulation for stimulating the high-quality development of high-proportion solar PV generation for carbon neutrality in countries including China.
Assessment of Nutrients in Natural Saltlicks, Artificial Saltlicks, and General Soils Used by Wild Asian Elephants (Elephas maximus) in the Western Forests of Thailand
Authors: Chaiyarat Rattanawat , Kanthachompoo Salisa , Thongtip Nikorn , Yuttitham Monthira
Published: December 2023

Saltlicks are fundamental resources for wild Asian elephants (Elephas maximus). This study aimed to assess the nutrients found in natural saltlicks (NSs) and artificial saltlicks (ASs), as well as general soils (GS) in the natural forest of Salakphra Wildlife Sanctuary (SWS) and a restoration area of Kui Buri National Park (KNP), a which is a forest in Western Thailand. We monitored 33 NSs, 35 ASs, and 20 GSs used by wild Asian elephants. In both areas, the K, Mg, Fe, and Cu in NSs were significantly higher than in ASs. The Ca and Zn in NSs of KNP were lower than the ASs of SWS. The salinity of ASs was the highest, making it significantly higher than that of the NSs in both areas. The ASs can supplement Na, thereby increasing salinity in both areas. The Ca, K, Mg, Fe, and Cu in NSs were significantly higher than in ASs, making them a primary target for elephants. These findings have consequences for conserving elephants and other large herbivores by supplementing essential macro- and micro-nutrients in ASs.
Improving Landslide Prediction: Innovative Modeling and Evaluation of Landslide Scenario with Knowledge Graph Embedding
Authors: Chen Luanjie , Peng Ling , Yang Lina
Published: December 2023

The increasing frequency and magnitude of landslides underscore the growing importance of landslide prediction in light of factors like climate change. Traditional methods, including physics-based methods and empirical methods, are beset by high costs and a reliance on expert knowledge. With the advancement of remote sensing and machine learning, data-driven methods have emerged as the mainstream in landslide prediction. Despite their strong generalization capabilities and efficiency, data-driven methods suffer from the loss of semantic information during training due to their reliance on a ‘sequence’ modeling method for landslide scenarios, which impacts their predictive accuracy. An innovative method for landslide prediction is proposed in this paper. In this paper, we propose an innovative landslide prediction method. This method designs the NADE ontology as the schema layer and constructs the data layer of the knowledge graph, utilizing tile lists, landslide inventory, and environmental data to enhance the representation of complex landslide scenarios. Furthermore, the transformation of the landslide prediction task into a link prediction task is carried out, and a knowledge graph embedding model is trained to achieve landslide predictions. Experimental results demonstrate that the method improves the F1 score by 5% in scenarios with complete datasets and 17% in scenarios with sparse datasets compared to data-driven methods. Additionally, the application of the knowledge graph embedding model is utilized to generate susceptibility maps, and an analysis of the effectiveness of entity embeddings is conducted, highlighting the potential of knowledge graph embeddings in disaster management.
Enhanced Micro-Doppler Feature Extraction Using Adaptive Short-Time Kernel-Based Sparse Time-Frequency Distribution
Authors: Yang Yang , Cheng Yongqiang , Wu Hao , Yang Zheng , Wang Hongqiang
Published: December 2023

The extraction of the micro-Doppler (m-D) feature based on time-frequency distribution (TFD) is of great significance for target detection and identification. To improve the feature extraction performance, numerous TFDs have been developed, with the majority falling under Cohen’s class. Nevertheless, these TFDs basically face a trade-off between artifact suppression and energy concentration. The main reason is that each Cohen’s class TFD is constructed by applying the two-dimensional Fourier transform to a kerneled ambiguity function directly, while existing kernels generally attenuate artifacts at the expense of losing valuable information. In this paper, a TFD reconstruction method employing an adaptive short-time kernel (ASTK) is developed in the framework of sparse representation (SR) theory to overcome this trade-off and enhance the m-D feature. Firstly, the task of the optimal kernel is explained from the viewpoint of the instantaneous auto-correlation function (IAF). Secondly, based on the quasi-linear frequency modulation feature of most m-D signals during short-time periods, the distribution rule of the short-time IAF (STIAF) in the ambiguity plane is concluded. Guided by this rule, an ASTK that can effectively remove unwanted artifacts with the least information loss is designed. Finally, an SR-based reconstruction procedure is conducted on the kerneled STIAF to generate an artifact-free TFD with high energy concentration, which can effectively enhance the m-D feature. Experiments using both simulated and real-world m-D signals demonstrate the effectiveness of the proposed method.
STELO: A New Modular Robotic Gait Device for Acquired Brain Injury—Exploring Its Usability
Authors: Cumplido-Trasmonte Carlos , Barquín-Santos Eva , Gor-García-Fogeda María Dolores , Plaza-Flores Alberto , García-Varela David , Ibáñez-Herrán Leticia , González-Alted Carlos , Díaz-Valles Paola , López-Pascua Cristina , Castrillo-Calvillo Arantxa , Molina-Rueda Francisco , Fernandez Roemi , Garcia-Armada Elena
Published: December 2023

In recent years, the prevalence of acquired brain injury (ABI) has been on the rise, leading to impaired gait functionality in affected individuals. Traditional gait exoskeletons are typically rigid and bilateral and lack adaptability. To address this, the STELO, a pioneering modular gait-assistive device, was developed. This device can be externally configured with joint modules to cater to the diverse impairments of each patient, aiming to enhance adaptability and efficiency. This study aims to assess the safety and usability of the initial functional modular prototype, STELO, in a sample of 14 ABI-diagnosed participants. Adverse events, device adjustment assistance and time, and gait performance were evaluated during three sessions of device use. The results revealed that STELO was safe, with no serious adverse events reported. The need for assistance and time required for device adjustment decreased progressively over the sessions. Although there was no significant improvement in walking speed observed after three sessions of using STELO, participants and therapists reported satisfactory levels of comfort and usability in questionnaires. Overall, this study demonstrates that the STELO modular device offers a safe and adaptable solution for individuals with ABI, with positive user and therapist feedback.