bluuebunny/crossref_metadata_2025 huggingface dataset:
The Election Commission of India: Guardian of Democracy
Authors: Ahuja Amit , Ostermann Susan
Published: November 2020

This chapter tells the story of how the Election Commission of India (ECI) became one of the most awe-inspiring electoral regulatory bodies in the world. One of the most widely celebrated and trusted public institutions in India, it has ensured the integrity—free and fair—of 17 national and more than 370 state elections since 1947, in what is not only the most populous but also one of the most potentially fractious democracies in the world. Ever under pressure from the executive branch and governing parties to bow to demands fed by their desire for electoral windfalls, the ECI managed to strengthen its autonomy through assertive leadership by a series of Chief Electoral Commissioners following the decline of the Congress Party’s political dominance. The rise of the Hindu Nationalist BJP as the new dominant force in Indian politics provides a crucial test for the endurance of the ECI’s role as India’s guardian of electoral integrity.
The BBC: Guardian of Public Understanding
Authors: Seaton Jean
Published: November 2020

The British Broadcasting Corporation (BBC) is a long-standing institution with a worldwide reputation as the maker and supplier of trustworthy news embedded in programmes aimed at serving the public not commoditising it. This chapter describes the BBC’s institutional DNA and explains how its birth characteristics informed its institutional trajectory over the decades. The chapter discusses the internal principles and the particular ‘craft’ that has made this a true British institution that has become equally revered outside Britain’s borders. It analyses how the BBC survived institutional crises to reach a moment in history where the very idea underlying this venerable but agile institution has come under fire.
The European Court of Justice: Guardian of European Integration
Authors: Boin Arjen , Schmidt Susanne K.
Published: November 2020

The European Court of Justice (ECJ) was founded in 1952. At that time, few observers would have predicted a future of a powerful institution, shaping Europe’s faith through its rulings. But that is exactly what happened. After a slow start, the ECJ gradually evolved into one of the most important institutions of the European Union. This chapter describes how this little court shrouded in secrecy managed to climb the institutional ladder. It analyses the role of institutional leadership, the relation between the ECJ and its authorizing environment, the near-existential crisis it faced in the 1970s, and the potential vulnerabilities that have grown over the years.
The World Anti-Doping Agency: Guardian of Elite Sport’s Credibility
Authors: van Bottenburg Maarten , Geeraert Arnout , de Hon Olivier
Published: November 2020

WADA is a hybrid public–private agency that leads the global fight against doping. This chapter explores why and how this agency developed into an institution that receives support from sports organizations and governments worldwide. Despite initial scepticism about its ability to overcome the ineffectiveness of anti-doping policies prior to its foundation in 1999, WADA quickly grew into a broadly trusted and well-respected organization. It successfully developed a globally harmonized anti-doping system that reinforced the credibility of international sports competitions and the legitimacy of elite sport policies. From its inception, it had a distinct identity as a neutral, impartial and objective standard setter and referee agent in a morally challenging organizational field. Nonetheless, being relatively young, WADA remains a vulnerable institution. It must continuously take an independent position with regard to partial interests of sporting and public authorities that are responsible for WADA’s funding and governance. This requires institutional leadership that the organization cannot always offer, as recent doping affairs show.
CERN: Guardian of the Human Aspiration to Understand the Universe
Authors: Engelen Jos , ‘t Hart Paul
Published: November 2020

The European Organization for Nuclear Research (CERN) is the world’s most formidable centre for particle physics. Its mission is radically ambitious: uncovering what the universe is made of and how it works. It advances that mission by providing particle accelerator facilities that enable world-class research in fundamental physics, bringing together scientists from all over the world to push the frontiers of science and technology. It has become widely recognized as one of the most successful cross-national collaborative research organizations of all times. Smart institutional design, good governance, resourceful leadership and resilient collaboration have underpinned the strong sense of interdependence, entrenched norms of mutual respect, trust, empathy and consensual decision-making that have allowed it to thrive.
Surface operators in superspace
Authors: Cremonini C. A. , Grassi P. A. , Penati S.
Published: November 2020

Abstract

We generalize the geometrical formulation of Wilson loops recently introduced in [1] to the description of Wilson Surfaces. For N = (2, 0) theory in six dimensions, we provide an explicit derivation of BPS Wilson Surfaces with non-trivial coupling to scalars, together with their manifestly supersymmetric version. We derive explicit conditions which allow to classify these operators in terms of the number of preserved supercharges. We also discuss kappa-symmetry and prove that BPS conditions in six dimensions arise from kappa-symmetry invariance in eleven dimensions. Finally, we discuss super-Wilson Surfaces — and higher dimensional operators — as objects charged under global p-form (super)symmetries generated by tensorial supercurrents. To this end, the construction of conserved supercurrents in supermanifolds and of the corresponding conserved charges is developed in details.
Intraspecific macroscopic digestive anatomy of ring-tailed lemurs (Lemur catta), including a comparison of frozen and formalin-stored specimens
Authors: Clauss Marcus , Trümpler Jelscha , Ackermans Nicole L. , Kitchener Andrew C. , Hantke Georg , Stagegaard Julia , Takano Tomo , Shintaku Yuta , Matsuda Ikki
Published: November 2020

Digestive tract measurements are often considered species specific, but little information exists on the degree to which they change during ontogeny within a species. Additionally, access to anatomical material from nondomestic species is often limited, with fixed tissues possibly representing the only available source, though the degree to which this material is representative in terms of dimensions and weight is debatable. In the present study, the macroscopic anatomy of the digestive tract (length of intestinal sections, and tissue weights of stomach and intestines) of 58Lemur catta[ranging in age from 1 month (neonates) to 25 years], which had been stored frozen (n = 27) or fixed in formalin (n = 31), was quantified. Particular attention was paid to the caecum and the possible presence of an appendix. The intraspecific allometric scaling of body mass (BM)0.46[0.40;0.51]for total intestine length and BM0.48[0.41;0.54]for small intestine length was higher than the expected geometric scaling of BM0.33, and similar to that reported in the literature for interspecific scaling. This difference in scaling is usually explained by the hypothesis that, to maintain optimal absorption, the diameter of the intestinal tube cannot increase geometrically. Therefore, geometric volume gain of increasing body mass is accommodated for by more-than-geometric length scaling. According to the literature, not allL. cattahave an appendix. No appendix was found in the specimens in the present study. The proportions of length measurements did not change markedly during ontogeny, indicating that the proportions of the foetus are representative of those of the adult animal. By contrast, width and tissue-mass scaling of the caecum indicated disproportionate growth of this organ during ontogeny that was not reflected in its length. Compared to overall intraspecific variation, the method of storage (frozen vs. formalin) had no relevant impact on length or weight measurements.
Can sodium MRI be used as a method for mapping of cartilage stiffness?
Authors: Brinkhof Sander , Froeling Martijn , Janssen Rob P. A. , Ito Keita , Klomp Dennis W. J.
Published: November 2020

Objective

Sodium concentration is responsible for (at least part of) the stiffness of articular cartilage due to the osmotic pressure it generates. Therefore, we hypothesized that we could use sodium MRI to approximate the stiffness of cartilage to assess early cartilage degeneration.

Methods

Four human tibial plateaus were retrieved from patients undergoing total knee replacement (TKR), and their cartilage stiffness mapped with indentation testing, after which samples were scanned in a 7 T MRI to determine sodium concentration. The relation of biomechanical parameters to MRI sodium and glycosaminoglycan (GAG) concentration was explored by a linear mixed model.

Results

Weak correlations of GAG concentration with apparent peak modulus (p = 0.0057) and apparent equilibrium modulus (p = 0.0181) were observed and lack of correlation of GAG concentration versus MRI sodium concentration was observed. MRI sodium concentration was not correlated with apparent peak modulus, though a moderate correlation of MRI sodium concentration with permeability was shown (p = 0.0014).

Discussion and conclusion

Although there was correlation between GAG concentration and cartilage stiffness, this was not similar with sodium concentration as measured by MRI. Thus, if the correlation between MRI sodium imaging and GAG concentration could be resolved, this strategy for assessing cartilage functional quality still holds promise.
Stability of Drinking Reductions and Long-term Functioning Among Patients with Alcohol Use Disorder
Authors: Witkiewitz Katie , Kranzler Henry R. , Hallgren Kevin A. , Hasin Deborah S. , Aldridge Arnie P. , Zarkin Gary A. , Mann Karl F. , O’Malley Stephanie S. , Anton Raymond F.
Published: November 2020

Background

The World Health Organization (WHO) categorizes alcohol consumption according to grams consumed into low-, medium-, high-, and very-high-risk drinking levels (RDLs). Although abstinence has been considered the ideal outcome of alcohol treatment, reductions in WHO RDLs have been proposed as primary outcomes for alcohol use disorder (AUD) trials.

Objective

The current study examines the stability of WHO RDL reductions and the association between RDL reductions and long-term functioning for up to 3 years following treatment.

Design and Participants

Secondary data analysis of patients with AUD enrolled in the COMBINE Study and Project MATCH, two multi-site, randomized AUD clinical trials, who were followed for up to 3 years post-treatment (COMBINE: n = 694; MATCH: n = 806).

Measures

Alcohol use was measured via calendar-based methods. We estimated all models in the total sample and among participants who did not achieve abstinence during treatment.

Key Results

One-level RDL reductions were achieved by 84% of patients at the end of treatment, with 84.9% of those individuals maintaining that reduction at a 3-year follow-up. Two-level RDL reductions were achieved by 68% of patients at the end of treatment, with 77.7% of those individuals maintaining that reduction at a 3-year follow-up. One- and two-level RDL reductions at the end of treatment were associated with significantly better mental health, quality of life (including physical quality of life), and fewer drinking consequences 3 years after treatment (p < 0.05), as compared to no change or increased drinking.

Conclusion

AUD patients can maintain WHO RDL reductions for up to 3 years after treatment. Patients who had WHO RDL reductions functioned significantly better than those who did not reduce their drinking. These findings are consistent with prior reports suggesting that drinking reductions, short of abstinence, yield meaningful improvements in patient health, well-being, and functioning.
The ‘end of the world’ vs. the ‘end of the month’: understanding social resistance to sustainability transition agendas, a lesson from the Yellow Vests in France
Authors: Martin Mathilde , Islar Mine
Published: November 2020

This paper investigates the Yellow Vests movement and the extent it constitutes an original platform for resistance to a sustainability transition agenda in the French context. The movement represents a disruption to global climate mitigation solutions that are often formalized at a global scale, and illustrates cultural and economic constraints in providing social justice in the age of climate change. Using a cultural performative approach, this case study reveals the relevance of framing and cultural analyses to understand such resistance. This qualitative exploration initiates a narrative analysis to assess how the universal resolve of the 2015 Paris Conference and the related legitimacy of the sustainability discourse has been further contested by the Yellow Vests and their fractured framing. From the ‘end of the world’ to the ‘end of the month’, we investigate the rise and fall of the legitimacy of the French sustainability discourse by analysing politicians’ and activists’ speeches, historical narratives as well as visual materials of resistance in France in the context of sustainability transitions. Tip of a broader social crisis, the movement reveals an original conflict of temporalities, symptomatic of the inevitable interdependency of socio-economic inequalities to sustainability transitions. Beyond the resistance itself, the Yellow Vests embody an original exemplar for the importance of cultural appropriation within the sustainability discourse’ legitimation processes.
The African golden cat Caracal aurata in Tanzania: first record and vulnerability assessment
Authors: Greco Ilaria , Rovero Francesco
Published: November 2020

We report on the first population found in Tanzania of the Vulnerable African golden cat Caracal aurata, extending its documented range c. 200 km to the south and south-east. This is one of the least-known and truly forest-dependent felines in Africa, ranging across the Guinea–Congolian forest block. We recorded the new population in Minziro Nature Forest Reserve, north-west Tanzania, during a 3-month survey in 2018. We deployed 70 camera traps on a regular grid and obtained 33 detection events of the golden cat at 26% of sites, with a minimum of 10 individuals across 257 km2. We estimated occupancy and detection probability and modelled these in relation to the distance of sampling sites to the forest edge, which coincides with both the Reserve boundary and proximity to human settlements surrounding the Reserve. Mean estimated occupancy was 0.41 ± SE 0.12 (mean detectability = 0.13 ± SE 0.05), with occupancy increasing significantly with distance from the forest edge. Detectability did not vary significantly with distance from the forest edge, but was higher for camera models that had a shorter trigger time. Our findings add to the scant data available for this species. It appears threatened by human activity, which we recorded both outside and within the Reserve, and the presence of the species indicates Minziro Forest is an important site for its conservation.
Using the Rorschach as a Group Intervention to Promote the Understanding of Adolescents by Staff Members in Inpatient Residential Programs
Authors: Aschieri Filippo , Vetere Carlo
Published: November 2020

Abstract. Mental health professionals working in adolescent residential treatment facilities face various challenges in delivering effective treatment to their patients. Establishing therapeutic alliance is often regarded as particularly daunting. Adolescents’ acting-out behaviors, emotional dysregulation, and difficulty in trusting adults can trigger strong reactions in mental health professionals. These reactions may increase the risk that mental health professionals respond to adolescents’ behaviors in similarly unmodulated ways and become involved in a reciprocal pathological process. The Rorschach test can provide vivid imagery and response content that depicts the subjective dilemmas adolescents are struggling with during their recovery in the treatment center. In this paper we show how to use Rorschach imagery to allow the mental health professionals working in adolescent inpatient treatment centers to understand their patients and increase empathy in response to adolescents’ troubling behaviors. We illustrate this procedure through the case of an adolescent patient who engaged in severely disruptive acting-out behaviors with the treating staff. Even though the staff were considering terminating his treatment in the facility as the only viable option, they instead found new empathy and a way to work with him after discussion of his Rorschach led by the inpatient center psychologist.
The Thurston Cradock Test of Shame as a Crucial “Empathy Magnifier”
Authors: Cradock O’Leary Julie , Nakamura Noriko , Finn Stephen E.
Published: November 2020

Abstract. This article presents a Therapeutic Assessment (TA; Finn, 2007 ) case study of a teenage girl assessed because of a complex set of symptoms: history of suicidal statements, sensory sensitivity, questions about gender identity, depression, anxiety/OCD, and possible subclinical psychotic symptoms. Logan’s parents, Joe and Susan, participated in the assessment, with Susan attending every appointment and Joe attending only the first and last. Ten instruments were administered, with each providing information about different aspects of Logan. The Thurston Cradock Test of Shame (TCTS; Thurston & Cradock O’Leary, 2009 ) scores and analysis encompassed all of the other test findings, clarified details not fully explained by tests such as the Rorschach and MMPI-A, and brought the core adolescent and family issue of shame to the foreground. When the assessors explained shame to Logan’s mother, she was better able to understand her daughter’s shame and connect with her own feelings of shame. When Logan’s father arrived for the final feedback session, he was quite tense, argumentative, and threatened to cancel the appointment. The TCTS allowed the assessors to understand Joe’s aggressive behavior as signs of TCTS defenses of deflation, aggression, and inflation/contempt, appreciate his underlying feelings of shame, and to delicately restore the “interpersonal bridge” ( Kaufman, 1996 ), which is key to reducing shame. As a result, Joe’s defensiveness decreased, and he was open to continuing the feedback session. In the end, Joe was able to identify with his daughter in a new way and even admit his own sense of shame in the session. This provided an opportunity for healing in the whole family.
How Communication in Organizations Is Related to Foci of Commitment and Turnover Intentions
Authors: Holzwarth Sebastian , Gunnesch-Luca George , Soucek Roman , Moser Klaus
Published: November 2020

Abstract. The current study analyzes how two components of perceived organizational communication (vertical and horizontal) are related to employee turnover intentions via three types of affective commitment foci (organization, supervisor, and team). Using second-order confirmatory factor analysis and structural equation modeling techniques with a large cross-sectional dataset ( n = 3,317), our results show that, in line with social cohesion theory, vertical communication (e.g., supportiveness from the organization) is strongly related to affective organizational commitment, whereas horizontal communication (e.g., supportiveness from colleagues) is primarily related to affective team commitment. Additionally, both communication dimensions are related to affective supervisory commitment. Finally, these three foci of affective commitment incrementally explain and differentially mediate the relationship between perceived organizational communication and turnover intention.
Modeling Gravity Waves with Machine Learning
Authors: Wheeling Kate
Published: November 2020

Researchers used neural networks to better define the parameterizations necessary for modeling the distribution and characteristics of orographic gravity waves.
GLM Observations of the Brightest Lightning in the Americas
Authors: Peterson Michael , Lay Erin
Published: November 2020

Two years of Geostationary Lightning Mapper (GLM) science data are used to document the brightest lighting flashes observed on the Americas continent. The most radiant optical lightning emissions—termed “superbolts”—were first identified by the Vela satellite constellation in the 1970s (Turman, 1977, https://doi.org/10.1029/JC082i018p02566) and are defined in terms of peak optical power. GLM is an integrating sensor that, instead, measures the total optical energy from a lightning pulse. While GLM might not correctly classify short‐duration superbolts, its top lightning cases certainly fall in the superbolt category, and the wealth of GLM measurements over its stationary hemispheric field of view provide an unmatched sample of extraordinarily bright lightning. While radiant bolts in excess of 100× the optical energy of typical lightning are ubiquitous across the Americas and result from many types of lightning processes, we find the most radiant cases (>1,000×) are concentrated in the central United States and in the La Plata basin in South America. Coincident Earth Networks Global Lightning Network (ENGLN) observations reveal that these extremely bright emissions usually result from + CG strokes with high peak currents in long horizontal flashes outside of the convective core. Single cases of these megaflashes might produce multiple superbolts over their durations.
Reproductive potential does not cause loss of heat shock response performance in honey bees
Authors: Shih S. R. , Huntsman E. M. , Flores M. E. , Snow J. W.
Published: November 2020

In other species characterized to date, aging, as a function of reproductive potential, results in the breakdown of proteaostasis and a decreased capacity to mount responses by the heat shock response (HSR) and other proteostatic network pathways. Our understanding of the maintenance of stress pathways, such as the HSR, in honey bees, and in the reproductive queen in particular, is incomplete. Based on the findings in other species showing an inverse relationship between reproductive potential and HSR function, one might predict that that HSR function would be lost in the reproductive queens. However, as queens possess an atypical uncoupling of the reproduction-maintenance trade-off typically found in solitary organisms, HSR maintenance might also be expected. Here we demonstrate that reproductive potential does not cause loss of HSR performance in honey bees as queens induce target gene expression to levels comparable to those induced in attendant worker bees. Maintenance of HSR function with advent of reproductive potential is unique among invertebrates studied to date and provides a potential model for examining the molecular mechanisms regulating the uncoupling of the reproduction-maintenance trade-off in queen bees, with important consequences for understanding how stresses impact different types of individuals in honey bee colonies.
Citizen science via social media revealed conditions of symbiosis between a marine gastropod and an epibiotic alga
Authors: Kagawa Osamu , Uchida Shota , Yamazaki Daishi , Osawa Yumiko , Ito Shun , Chiba Satoshi ,   The green-costumed snail’s citizen researchers, Kagawa Emiko , Tamada Akihiro , Ishida So , Yoshida Junko , Kimura Kazuki , Iijima Akiko , Suenaga Takayuki , Momoi Teruaki , Kato You , Nikaido Satoshi , Kimura Taeko , Kobayashi Shingo , Niwa Kazuo , Nishi Hirotaka , Fujita Haruto , Kakihara Hideaki , Makino Shinichi , Suzuki Hiroe , Namikawa Akane , Yamakami Ryusei , Higashi Kanae , Watanabe Kota , Yoshimura Taro , Isotomo  , Sagara Mitsunori , Aoki Yuta , Sugimoto Ryoya
Published: November 2020

Environmental factors promote symbiosis, but its mechanism is not yet well understood. The algaPseudocladophora conchopheriagrows only on the shell of an intertidal gastropodLunella correensis, and these species have a close symbiotic relationship which the alga reduces heat stress of the gastropod. In collaboration with general public, we investigated how environmental conditions alter the symbiotic interaction between the alga and the gastropod. Information about the habitats of each gastropod and images of shells was obtained from the Japanese and Korean coasts via social media. We constructed the hierarchical Bayesian model using the data. The results indicated that the proportion of shell area covered byP. conchopheriaincreased as the substrate size utilized by the gastropod increased. Meanwhile, temperature did not affect the proportion ofP. conchopheriaon the shell. These suggested that the alga provides no benefits for the gastropod on small substrates because gastropod can reduce the heat stress by diving into the small sediment. Further, the gastropod’s cost incurred by growing the alga on the shell seems to be low as the algae can grow even in cooler places where no benefits of heat resistance for gastropods. Different environments can yield variable conditions in symbiosis.
Single-cell RNA sequencing confirms IgG transcription and limited diversity of VHDJH rearrangements in proximal tubular epithelial cells
Authors: Deng Zhenling , Wang Xinyao , Liu Yue , Tian Xinyu , Deng Shaohui , Sun Yingchun , Wang Song , Zheng Danxia , Cui Zhuan , Pan Yuejuan , A Lata , Yan Huige , Qiu Xiaoyan , Wang Yue
Published: November 2020

Increasing evidence has confirmed that immunoglobulins (Igs) can be expressed in non-B cells. Our previous work demonstrated that mesangial cells and podocytes express IgA and IgG, respectively. The aim of this work was to reveal whether proximal tubular epithelial cells (PTECs) express Igs. High-throughput single-cell RNA sequencing (scRNA-seq) detected Igs in a small number of PTECs, and then we combined nested PCR with Sanger sequencing to detect the transcripts and characterize the repertoires of Igs in PTECs. We sorted PTECs from the normal renal cortex of two patients with renal cancer by FACS and further confirmed their identify by LRP2 gene expression. Only the transcripts of the IgG heavy chain were successfully amplified in 91/111 single PTECs. We cloned and sequenced 469 VHDJH transcripts from 91 single PTECs and found that PTEC-derived IgG exhibited classic VHDJH rearrangements with nucleotide additions at the junctions and somatic hypermutations. Compared with B cell-derived IgG, PTEC-derived IgG displayed less diversity of VHDJH rearrangements, predominant VH1-24/DH2-15/JH4 sequences, biased VH1 usage, centralized VH gene segment location at the 3′ end of the genome and non-Gaussian distribution of the CDR3 length. These results demonstrate that PTECs can express a distinct IgG repertoire that may have implications for their role in the renal tubular epithelial-mesenchymal transition.
Hierarchical analysis of ontogenetic time to describe heterochrony and taxonomy of developmental stages
Authors: Lecointre Guillaume , Schnell Nalani K. , Teletchea Fabrice
Published: November 2020

Even though an accurate description of early life stages is available for some teleostean species in form of embryonic and post-embryonic developmental tables, there is poor overlap between species-specific staging vocabularies beyond the taxonomic family level. What is called “embryonic period”, “larval period”, “metamorphosis”, or “juvenile” is anatomically different across teleostean families. This problem, already pointed out 50 years ago, challenges the consistency of developmental biology, embryology, systematics, and hampers an efficient aquaculture diversification. We propose a general solution by producing a proof-of-concept hierarchical analysis of ontogenetic time using a set of four freshwater species displaying strongly divergent reproductive traits. With a parsimony analysis of a matrix where “operational taxonomic units” are species at a given ontogenetic time segment and characters are organs or structures which are coded present or absent at this time, we show that the hierarchies obtained have both very high consistency and retention index, indicating that the ontogenetic time is correctly grasped through a hierarchical graph. This allows to formally detect developmental heterochronies and might provide a baseline to name early life stages for any set of species. The present method performs a phylogenetic segmentation of ontogenetic time, which can be correctly seen as depicting ontophylogenesis.
Synthesis and molecular structure of perhalogenated rhenium-oxo corroles
Authors: Alemayehu Abraham B. , Einrem Rune F. , McCormick-McPherson Laura J. , Settineri Nicholas S. , Ghosh Abhik
Published: November 2020

As part of our efforts to develop rhenium-oxo corroles as photosensitizers for oxygen sensing and photodynamic therapy, we investigated the potential β-perhalogenation of five ReO meso-tris(para-X-phenyl)corroles, Re[TpXPC](O) (X = CF3, H, F, CH3, and OCH3), with elemental chlorine and bromine. With Cl2, β-octachlorinated products Re[Cl8TpXPC](O) were rapidly obtained for X = CF3, H, and CH3, but X = OCH3 resulted in overchlorination on the meso-aryl groups. Full β-octabromination proved slower relative to Cu and Ir corroles, but the desired Re[Br8TpXPC](O) products were finally obtained for X = H and F after a week at room temperature. For X = CH3 and OCH3, these conditions led to undecabrominated products Re[Br11TpXPC](O). Compared to the β-unsubstituted starting materials, the β-octahalogenated products were found to exhibit sharp 1H NMR signals at room temperature, indicating that the aryl groups are locked in place by the β-halogens, and substantially redshifted Soret and Q bands. Single-crystal X-ray structures of Re[Cl8TpCF3PC](O), Re[Cl8TpCH3PC](O), and Re[Br8TpFPC](O) revealed mild saddling for one Cl8 structure and the Br8 structure. These structural variations, however, appear too insignificant to explain the slowness of the β-octabromination protocols, which seems best attributed to the deactivating influence of the high-valent Re center.
Effects of humic acid on enhanced removal of lead ions by polystyrene-supported nano-Fe (0) nanocomposite
Authors: Wang Luyao , Wei Shiqiang , Jiang Zhenmao
Published: November 2020

Polymer-supported nanozero-valent iron composites (D001-nZVI) were fabricated for the removal of lead ions from aqueous solutions by embedding nZVI into the porous polystyrene anion exchanger D001. Humic acid (HA) was selected as a model species because of its ubiquitous existence to gain insight into the influencing factors in the actual application process. The iron contents of the composites were approximately 11.2%, and the smallest ZVI particle size was ~ 5 nm. The experimental results showed that the effect of HA on the reduction of lead ions by D001-nZVI was a concentration-dependent process. At low HA concentrations, the surface-competitive adsorption of HA and Pb2+ dominated; therefore, the removal efficiency of Pb2+ by D001-nZVI decreased from 97.5 to 90.2% with an increasing HA concentration. When the HA concentration increased to 30 mg/L or more, the lead ions removal remained constant with the following possible cooperation mechanism: the competitive adsorption of HA and Pb2+ on the nZVI surface and the well-dispersed particles were caused by electrostatic interactions between the HA coating and the nZVI surface. In addition, the adsorption complexation between HA and Pb2+ also had a positive effect on the removal of Pb2+ at higher concentrations of HA.
Antibiofilm effects of N,O-acetals derived from 2-amino-1,4-naphthoquinone are associated with downregulation of important global virulence regulators in methicillin-resistant Staphylococcus aureus
Authors: Novais Juliana Silva , Carvalho Mariana Fernandes , Ramundo Mariana Severo , Beltrame Cristiana Ossaille , Geraldo Reinaldo Barros , Jordão Alessandro Kappel , Ferreira Vítor Francisco , Castro Helena Carla , Figueiredo Agnes Marie Sá
Published: November 2020

Despite the existing antibiotics, antimicrobial resistance is a major challenge. Consequently, the development of new drugs remains in great demand. Quinones is part of a broad group of molecules that present antibacterial activity besides other biological properties. The main purpose of this study was to evaluate the antibiofilm activities of synthetic N,O-acetals derived from 2-amino-1,4-naphthoquinone [7a: 2-(methoxymethyl)-amino-1,4-naphthoquinone; 7b: 2-(ethoxymethyl)-amino-1,4-naphthoquinone; and 7c: 2-(propynyloxymethyl)-amino-1,4-naphthoquinone] against methicillin-resistant Staphylococcus aureus (MRSA). The derivatives 7b and 7c, specially 7b, caused strong impact on biofilm accumulation. This inhibition was linked to decreased expression of the genes fnbA, spa, hla and psmα3. More importantly, this downregulation was paralleled by the modulation of global virulence regulators. The substitution of 2-ethoxymethyl (7b) in comparison with 2-propynyloxymethyl (7c) enhanced sarA-agr inhibition, decreased fnbA transcripts (positively regulated by sarA) and strongly impaired biofilm accumulation. Indeed, 7b triggered intensive autolysis and was able to eliminate vancomycin-persistent cells. Consequently, 7b is a promising molecule displaying not only antimicrobial effects, but also antibiofilm and antipersistence activities. Therefore, 7b is a good candidate for further studies involving the development of novel and more rational antimicrobials able to act in chronic and recalcitrant infections, associated with biofilm formation.
Production of synthetic wheat lines to exploit the genetic diversity of emmer wheat and D genome containing Aegilops species in wheat breeding
Authors: Mirzaghaderi Ghader , Abdolmalaki Zinat , Ebrahimzadegan Rahman , Bahmani Farshid , Orooji Fatemeh , Majdi Mohammad , Mozafari Ali-Akbar
Published: November 2020

Due to the accumulation of various useful traits over evolutionary time, emmer wheat (Triticum turgidumsubsp.dicoccumanddicoccoides, 2n = 4x = 28; AABB), durum wheat (T. turgidumsubsp.durum, 2n = 4x = 28; AABB),T. timopheevii(2n = 4x = 28; AAGG) and D genome containingAegilopsspecies offer excellent sources of novel variation for the improvement of bread wheat (T. aestivumL., AABBDD). Here, we made 192 different cross combinations between diverse genotypes of wheat andAegilopsspecies including emmer wheat × Ae. tauschii(2n = DD or DDDD), durum wheat × Ae. tauschii,T. timopheevii × Ae. tauschii,Ae. crassa × durum wheat,Ae. cylindrica × durum wheat andAe. ventricosa × durum wheat in the field over three successive years. We successfully recovered 56 different synthetic hexaploid and octaploid F2lines with AABBDD, AABBDDDD, AAGGDD, D1D1XcrXcrAABB, DcDcCcCcAABB and DvDvNvNvAABB genomes via in vitro rescue of F1embryos and spontaneous production of F2seeds on the Flplants. Cytogenetic analysis of F2lines showed that the produced synthetic wheat lines were generally promising stable amphiploids. Contribution of D genome bearingAegilopsand the less-investigated emmer wheat genotypes as parents in the crosses resulted in synthetic amphiploids which are a valuable resource for bread wheat breeding.
Digital holographic microscopy for real-time observation of surface-relief grating formation on azobenzene-containing films
Authors: Rekola Heikki , Berdin Alex , Fedele Chiara , Virkki Matti , Priimagi Arri
Published: November 2020

Light-induced surface structuring of azobenzene-containing films allows for creation of complex surface relief patterns with varying heights, patterns which would be difficult to create using conventional lithography tools. In order to realize the full potential of these patternable surfaces, understanding their formation dynamics and response to different types of light fields is crucial. In the present work we introduce digital holographic microscopy (DHM) for real time, in-situ observation of surface-relief grating (SRG) formation on azobenzene-containing films. This instrument allows us to measure the surface topography of films while illuminating them with two individually controlled laser beams for creating periodically varying patterns. By utilizing the information of the grating formation dynamics, we combine multiple grating patterns to create pixels with wide gamut structural colors as well as blazed grating structures on the film surface. As long as the material behaviour is linear, any Fourier optical surface can be created utilizing this multiple patterning approach. The DHM instrument presented here has the potential for creating complex 3D surface reliefs with nanometric precision.
Structure of Nora virus at 2.7 Å resolution and implications for receptor binding, capsid stability and taxonomy
Authors: Laurinmäki Pasi , Shakeel Shabih , Ekström Jens-Ola , Mohammadi Pezhman , Hultmark Dan , Butcher Sarah J.
Published: November 2020

Nora virus, a virus of Drosophila, encapsidates one of the largest single-stranded RNA virus genomes known. Its taxonomic affinity is uncertain as it has a picornavirus-like cassette of enzymes for virus replication, but the capsid structure was at the time for genome publication unknown. By solving the structure of the virus, and through sequence comparison, we clear up this taxonomic ambiguity in the invertebrate RNA virosphere. Despite the lack of detectable similarity in the amino acid sequences, the 2.7 Å resolution cryoEM map showed Nora virus to have T = 1 symmetry with the characteristic capsid protein β-barrels found in all the viruses in the Picornavirales order. Strikingly, α-helical bundles formed from the extended C-termini of capsid protein VP4B and VP4C protrude from the capsid surface. They are similar to signalling molecule folds and implicated in virus entry. Unlike other viruses of Picornavirales, no intra-pentamer stabilizing annulus was seen, instead the intra-pentamer stability comes from the interaction of VP4C and VP4B N-termini. Finally, intertwining of the N-termini of two-fold symmetry-related VP4A capsid proteins and RNA, provides inter-pentamer stability. Based on its distinct structural elements and the genetic distance to other picorna-like viruses we propose that Nora virus, and a small group of related viruses, should have its own family within the order Picornavirales.
Asthma prevalence, lung and cardiovascular function in adolescents born preterm
Authors: Arroyas Maria , Calvo Cristina , Rueda Santiago , Esquivias Maria , Gonzalez-Menchen Cristina , Gonzalez-Carrasco Ersilia , Garcia-Garcia Maria Luz
Published: November 2020

Our main objective was to study respiratory evolution and pulmonary and cardiac function in adolescents born preterm in the post-surfactant era. Observational cross-sectional study, comparing very preterm (< 32 weeks) and moderately-late preterm adolescents (≥ 32 weeks) (74 each group). We recorded respiratory symptoms, spirometry and functional echocardiogram. Very preterm adolescents required more respiratory admissions (45.9% vs. 28.4%) (p = 0.03, OR 2.1, CI95% 1.1–4.2) and had more current asthma (21.6% vs. 9.5%, p = 0.04, OR 2.3, CI95% 1.1–5.2). Preterm subjects with intrauterine growth restriction (IUGR) presented lower FEV1 (88.7 ± 13.9 vs. 95.9 ± 13.3, p = 0.027) and lower FVC (88.2 ± 13.6 vs. 95.5 ± 13.3, p = 0.025). When assessing right ventricle, very preterm showed a greater E/E’ ratio (p = 0.02) and longer myocardial performance index (MPI) (p = 0.001). Adolescents with IUGR showed less shortening fraction (p = 0.016), worse E/E′ ratio (p = 0.029) and longer MPI (p = 0.06). Regarding left ventricle, very preterm showed less E′ wave velocity (p = 0.03), greater E/E′ ratio (p = 0.005) and longer MPI (p < 0.001). Gestational age < 32 weeks is independently associated with current asthma in adolescence. Children 13–14 years old born very preterm required more respiratory admissions and had poorer diastolic and global function of both ventricles. IUGR is a risk factor for poorer lung function in preterm adolescents, regardless gestational age.
Efficient solution of Boolean satisfiability problems with digital memcomputing
Authors: Bearden Sean R. B. , Pei Yan Ru , Di Ventra Massimiliano
Published: November 2020

Boolean satisfiability is a propositional logic problem of interest in multiple fields, e.g., physics, mathematics, and computer science. Beyond a field of research, instances of the SAT problem, as it is known, require efficient solution methods in a variety of applications. It is the decision problem of determining whether a Boolean formula has a satisfying assignment, believed to require exponentially growing time for an algorithm to solve for the worst-case instances. Yet, the efficient solution of many classes of Boolean formulae eludes even the most successful algorithms, not only for the worst-case scenarios, but also for typical-case instances. Here, we introduce a memory-assisted physical system (a digital memcomputing machine) that, when its non-linear ordinary differential equations are integrated numerically, shows evidence for polynomially-bounded scalability while solving “hard” planted-solution instances of SAT, known to require exponential time to solve in the typical case for both complete and incomplete algorithms. Furthermore, we analytically demonstrate that the physical system can efficiently solve the SAT problem in continuous time, without the need to introduce chaos or an exponentially growing energy. The efficiency of the simulations is related to the collective dynamical properties of the original physical system that persist in the numerical integration to robustly guide the solution search even in the presence of numerical errors. We anticipate our results to broaden research directions in physics-inspired computing paradigms ranging from theory to application, from simulation to hardware implementation.
Neurotrophic effects of dental pulp stem cells on trigeminal neuronal cells
Authors: Sultan Nessma , Amin Laila E. , Zaher Ahmed R. , Grawish Mohammed E. , Scheven Ben A.
Published: November 2020

Evidence indicates that dental pulp stem cells (DPSC) secrete neurotrophic factors which play an important role in neurogenesis, neural maintenance and repair. In this study we investigated the trophic potential of DPSC-derived conditioned medium (CM) to protect and regenerate isolated primary trigeminal ganglion neuronal cells (TGNC). DPSC and TGNC were harvested by enzymatic digestion from Wister-Hann rats. CM was collected from 72 h serum-free DPSC cultures and neurotrophic factors; nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and glial cell line-derived neurotrophic factor (GDNF) were analysed by specific enzyme-linked immunosorbent assays (ELISAs). Primary co-cultures of DPSC and TGNC were established to evaluate the paracrine effects of DPSC. In comparison, NGF was used to evaluate its neurotrophic and neuritogenic effect on TGNC. Immunocytochemistry was performed to detect the neuronal-markers; neuronal nuclei (NeuN), microtubule-associated protein-2 (MAP-2) and βIII-tubulin. Quantitative real time polymerase chain reaction (qRT-PCR) was used to analyse neuronal-associated gene expression of NeuN, MAP-2, βIII-tubulin in addition to growth-associated protein-43 (GAP-43), Synapsin-I and thermo-sensitive transient receptor potential vanilloid channel-1 (TRPV1). DPSC-CM contained significant levels of NGF, BDNF, NT-3 and GDNF. DPSC and DPSC-CM significantly enhanced TGNC survival with extensive neurite outgrowth and branching as evaluated by immunocytochemistry of neuronal markers. DPSC-CM was more effective in stimulating TGNC survival than co-cultures or NGF treated culture. In comparison to controls, DPSC-CM significantly upregulated gene expression of several neuronal markers as well as TRPV1. This study demonstrated that DPSC-derived factors promoted survival and regeneration of isolated TGNC and may be considered as cell-free therapy for TG nerve repair.
Withaferin A activates TRIM16 for its anti-cancer activity in melanoma
Authors: Nagy Zsuzsanna , Cheung Belamy B. , Tsang Wing , Tan Owen , Herath Mika , Ciampa Olivia C. , Shadma Fatima , Carter Daniel R. , Marshall Glenn M.
Published: November 2020

Although selective BRAF inhibitors and novel immunotherapies have improved short-term treatment responses in metastatic melanoma patients, acquired resistance to these therapeutics still represent a major challenge in clinical practice. In this study, we evaluated the efficacy of Withaferin A (WFA), derived from the medicinal plant Withania Somnifera, as a novel therapeutic agent for the treatment of melanoma. WFA showed selective toxicity to melanoma cells compared to non-malignant cells. WFA induced apoptosis, significantly reduced cell proliferation and inhibited migration of melanoma cells. We identified that repression of the tumour suppressor TRIM16 diminished WFA cytotoxicity, suggesting that TRIM16 was in part responsible for the cytotoxic effects of WFA in melanoma cells. Together our data indicates that WFA has potent cytopathic effects on melanoma cells through TRIM16, suggesting a potential therapeutic application of WFA in the disease.
Intra-individual variability of eGFR trajectories in early diabetic kidney disease and lack of performance of prognostic biomarkers
Authors: Kerschbaum Julia , Rudnicki Michael , Dzien Alexander , Dzien-Bischinger Christine , Winner Hannes , Heerspink Hiddo Lambers , Rosivall László , Wiecek Andrzej , Mark Patrick B. , Eder Susanne , Denicolò Sara , Mayer Gert
Published: November 2020

Studies reporting on biomarkers aiming to predict adverse renal outcomes in patients with type 2 diabetes and kidney disease (DKD) conventionally define a surrogate endpoint either as a percentage of decrease of eGFR (e.g. ≥ 30%) or an absolute decline (e.g. ≥ 5 ml/min/year). The application of those study results in clinical practise however relies on the assumption of a linear and intra-individually stable progression of DKD. We studied 860 patients of the PROVALID study and 178 of an independent population with a relatively preserved eGFR at baseline and at least 5 years of follow up. Individuals with a detrimental prognosis were identified using various thresholds of a percentage or absolute decline of eGFR after each year of follow up. Next, we determined how many of the patients met the same criteria at other points in time. Interindividual eGFR decline was highly variable but in addition intra-individual eGFR trajectories also were frequently non-linear. For example, of all subjects reaching an endpoint defined as a decrease of eGFR by ≥ 30% between baseline and 3 years of follow up, only 60.3 and 45.2% lost at least the same amount between baseline and year 4 or 5. The results were similar when only patients on stable medication or subpopulations based on baseline eGFR or albuminuria status were analyzed or an eGFR decline of ≥ 5 ml/min/1.73m2/year was used. Identification of reliable biomarkers predicting adverse prognosis is a strong clinical need given the large interindividual variability of DKD progression. However, it is conceptually challenging in early DKD because of non-linear intra-individual eGFR trajectories. As a result, the performance of a prognostic biomarker may be accurate after a specific time of follow-up in a single population only.