bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanosensitivity Is a Characteristic Feature of Cultured Suburothelial Interstitial Cells of the Human Bladder
Authors: Neuhaus Jochen , Gonsior Andreas , Cheng Sheng , Stolzenburg Jens-Uwe , Berger Frank Peter
Published: August 2020

Bladder dysfunction is characterized by urgency, frequency (pollakisuria, nocturia), and dysuria and may lead to urinary incontinence. Most of these symptoms can be attributed to disturbed bladder sensitivity. There is growing evidence that, besides the urothelium, suburothelial interstitial cells (suICs) are involved in bladder afferent signal processing. The massive expansion of the bladder during the filling phase implicates mechanical stress delivered to the whole bladder wall. Little is known about the reaction of suICs upon mechanical stress. Therefore, we investigated the effects of mechanical stimulation in cultured human suICs. We used fura-2 calcium imaging as a major physiological readout. We found spontaneous intracellular calcium activity in 75 % of the cultured suICs. Defined local pressure application via a glass micropipette led to local increased calcium activity in all stimulated suICs, spreading over the whole cell. A total of 51% of the neighboring cells in a radius of up to 100 µm from the stimulated cell showed an increased activity. Hypotonic ringer and shear stress also induced calcium transients. We found an 18-times increase in syncytial activity compared to unstimulated controls, resulting in an amplification of the primary calcium signal elicited in single cells by 50%. Our results speak in favor of a high sensitivity of suICs for mechanical stress and support the view of a functional syncytium between suICs, which can amplify and distribute local stimuli. Previous studies of connexin expression in the human bladder suggest that this mechanism could also be relevant in normal and pathological function of the bladder in vivo.
Differential Effects of Constant Light and Dim Light at Night on the Circadian Control of Metabolism and Behavior
Authors: Rumanova Valentina S. , Okuliarova Monika , Zeman Michal
Published: August 2020

The disruption of circadian rhythms by environmental conditions can induce alterations in body homeostasis, from behavior to metabolism. The light:dark cycle is the most reliable environmental agent, which entrains circadian rhythms, although its credibility has decreased because of the extensive use of artificial light at night. Light pollution can compromise performance and health, but underlying mechanisms are not fully understood. The present review assesses the consequences induced by constant light (LL) in comparison with dim light at night (dLAN) on the circadian control of metabolism and behavior in rodents, since such an approach can identify the key mechanisms of chronodisruption. Data suggest that the effects of LL are more pronounced compared to dLAN and are directly related to the light level and duration of exposure. Dim LAN reduces nocturnal melatonin levels, similarly to LL, but the consequences on the rhythms of corticosterone and behavioral traits are not uniform and an improved quantification of the disrupted rhythms is needed. Metabolism is under strong circadian control and its disruption can lead to various pathologies. Moreover, metabolism is not only an output, but some metabolites and peripheral signal molecules can feedback on the circadian clockwork and either stabilize or amplify its desynchronization.
Corynebacterium glutamicum CrtR and Its Orthologs in Actinobacteria: Conserved Function and Application as Genetically Encoded Biosensor for Detection of Geranylgeranyl Pyrophosphate
Authors: Henke Nadja A. , Austermeier Sophie , Grothaus Isabell L. , Götker Susanne , Persicke Marcus , Peters-Wendisch Petra , Wendisch Volker F.
Published: August 2020

Carotenoid biosynthesis in Corynebacteriumglutamicum is controlled by the MarR-type regulator CrtR, which represses transcription of the promoter of the crt operon (PcrtE) and of its own gene (PcrtR). Geranylgeranyl pyrophosphate (GGPP), and to a lesser extent other isoprenoid pyrophosphates, interfere with the binding of CrtR to its target DNA in vitro, suggesting they act as inducers of carotenoid biosynthesis. CrtR homologs are encoded in the genomes of many other actinobacteria. In order to determine if and to what extent the function of CrtR, as a metabolite-dependent transcriptional repressor of carotenoid biosynthesis genes responding to GGPP, is conserved among actinobacteria, five CrtR orthologs were characterized in more detail. EMSA assays showed that the CrtR orthologs from Corynebacteriumcallunae, Acidipropionibacteriumjensenii, Paenarthrobacternicotinovorans, Micrococcusluteus and Pseudarthrobacterchlorophenolicus bound to the intergenic region between their own gene and the divergently oriented gene, and that GGPP inhibited these interactions. In turn, the CrtR protein from C. glutamicum bound to DNA regions upstream of the orthologous crtR genes that contained a 15 bp DNA sequence motif conserved between the tested bacteria. Moreover, the CrtR orthologs functioned in C. glutamicum in vivo at least partially, as they complemented the defects in the pigmentation and expression of a PcrtE_gfpuv transcriptional fusion that were observed in a crtR deletion mutant to varying degrees. Subsequently, the utility of the PcrtE_gfpuv transcriptional fusion and chromosomally encoded CrtR from C. glutamicum as genetically encoded biosensor for GGPP was studied. Combined FACS and LC-MS analysis demonstrated a correlation between the sensor fluorescent signal and the intracellular GGPP concentration, and allowed us to monitor intracellular GGPP concentrations during growth and differentiate between strains engineered to accumulate GGPP at different concentrations.
Proteomic Research in Peritoneal Dialysis
Authors: Bonomini Mario , Borras Francesc E. , Troya-Saborido Maribel , Carreras-Planella Laura , Di Liberato Lorenzo , Arduini Arduino
Published: August 2020

Peritoneal dialysis (PD) is an established home care, cost-effective renal replacement therapy (RRT), which offers several advantages over the most used dialysis modality, hemodialysis. Despite its potential benefits, however, PD is an under-prescribed method of treating uremic patients. Infectious complications (primarily peritonitis) and bio-incompatibility of PD solutions are the main contributors to PD drop-out, due to their potential for altering the functional and anatomical integrity of the peritoneal membrane. To improve the clinical outcome of PD, there is a need for biomarkers to identify patients at risk of PD-related complications and to guide personalized interventions. Several recent studies have shown that proteomic investigation may be a powerful tool in the prediction, early diagnosis, prognostic assessment, and therapeutic monitoring of patients on PD. Indeed, analysis of the proteome present in PD effluent has uncovered several proteins involved in inflammation and pro-fibrotic insult, in encapsulating peritoneal sclerosis, or even in detecting early changes before any measurable modifications occur in the traditional clinical parameters used to evaluate PD efficacy. We here review the proteomic studies conducted thus far, addressing the potential use of such omics methodology in identifying potential new biomarkers of the peritoneal membrane welfare in relation to dialytic prescription and adequacy.
Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice
Authors: Jung Yu Jin , Kim Jong Hee , Lee Hyo Ju , Kim Dong Hyun , Yu Jihyeon , Bae Sangsu , Cho Yong-Gu , Kang Kwon Kyoo
Published: August 2020

The rice SLR1 gene encodes the DELLA protein (protein with DELLA amino acid motif), and a loss-of-function mutation is dwarfed by inhibiting plant growth. We generate slr1-d mutants with a semi-dominant dwarf phenotype to target mutations of the DELLA/TVHYNP domain using CRISPR/Cas9 genome editing in rice. Sixteen genetic edited lines out of 31 transgenic plants were generated. Deep sequencing results showed that the mutants had six different mutation types at the target site of the TVHYNP domain of the SLR1 gene. The homo-edited plants selected individuals without DNA (T-DNA) transcribed by segregation in the T1 generation. The slr1-d7 and slr1-d8 plants caused a gibberellin (GA)-insensitive dwarf phenotype with shrunken leaves and shortened internodes. A genome-wide gene expression analysis by RNA-seq indicated that the expression levels of two GA-related genes, GA20OX2 (Gibberellin oxidase) and GA3OX2, were increased in the edited mutant plants, suggesting that GA20OX2 acts as a convert of GA12 signaling. These mutant plants are required by altering GA responses, at least partially by a defect in the phytohormone signaling system process and prevented cell elongation. The new mutants, namely, the slr1-d7 and slr1-d8 lines, are valuable semi-dominant dwarf alleles with potential application value for molecule breeding using the CRISPR/Cas9 system in rice.
The Role of Pre-Clinical 3-Dimensional Models of Osteosarcoma
Authors: Smith Hannah L. , Beers Stephen A. , Gray Juliet C. , Kanczler Janos M.
Published: August 2020

Treatment for osteosarcoma (OS) has been largely unchanged for several decades, with typical therapies being a mixture of chemotherapy and surgery. Although therapeutic targets and products against cancer are being continually developed, only a limited number have proved therapeutically active in OS. Thus, the understanding of the OS microenvironment and its interactions are becoming more important in developing new therapies. Three-dimensional (3D) models are important tools in increasing our understanding of complex mechanisms and interactions, such as in OS. In this review, in vivo animal models, in vitro 3D models and in ovo chorioallantoic membrane (CAM) models, are evaluated and discussed as to their contribution in understanding the progressive nature of OS, and cancer research. We aim to provide insight and prospective future directions into the potential translation of 3D models in OS.
Flexible NAD+ Binding in Deoxyhypusine Synthase Reflects the Dynamic Hypusine Modification of Translation Factor IF5A
Authors: Chen Meirong , Gai Zuoqi , Okada Chiaki , Ye Yuxin , Yu Jian , Yao Min
Published: August 2020

The eukaryotic and archaeal translation factor IF5A requires a post-translational hypusine modification, which is catalyzed by deoxyhypusine synthase (DHS) at a single lysine residue of IF5A with NAD+ and spermidine as cofactors, followed by hydroxylation to form hypusine. While human DHS catalyzed reactions have been well characterized, the mechanism of the hypusination of archaeal IF5A by DHS is not clear. Here we report a DHS structure from Pyrococcus horikoshii OT3 (PhoDHS) at 2.2 Å resolution. The structure reveals two states in a single functional unit (tetramer): two NAD+-bound monomers with the NAD+ and spermidine binding sites observed in multi-conformations (closed and open), and two NAD+-free monomers. The dynamic loop region V288–P299, in the vicinity of the active site, adopts different positions in the closed and open conformations and is disordered when NAD+ is absent. Combined with NAD+ binding analysis, it is clear that PhoDHS can exist in three states: apo, PhoDHS-2 equiv NAD+, and PhoDHS-4 equiv NAD+, which are affected by the NAD+ concentration. Our results demonstrate the dynamic structure of PhoDHS at the NAD+ and spermidine binding site, with conformational changes that may be the response to the local NAD+ concentration, and thus fine-tune the regulation of the translation process via the hypusine modification of IF5A.
Aesculetin Attenuates Alveolar Injury and Fibrosis Induced by Close Contact of Alveolar Epithelial Cells with Blood-Derived Macrophages via IL-8 Signaling
Authors: Oh Su Yeon , Kim Yun-Ho , Kang Min-Kyung , Lee Eun-Jung , Kim Dong Yeon , Oh Hyeongjoo , Kim Soo-Il , Na Woojin , Kang Young-Hee
Published: August 2020

Pulmonary fibrosis is a disease in which lung tissues become fibrous and thereby causes severe respiratory disturbances. Various stimuli induce infiltration of macrophages to the respiratory tract, secreting inflammatory cytokines, which subsequently leads to the development of pulmonary fibrosis. Aesculetin, a major component of the sancho tree and chicory, is known to biologically have antioxidant and anti-inflammatory effects. Human alveolar epithelial A549 cells were cultured for 24 h in conditioned media of THP-1 monocyte-derived macrophages (mCM) with 1–20 μM aesculetin. Micromolar aesculetin attenuated the cytotoxicity of mCM containing inflammatory tumor necrosis factor-α (TNF)-α and interleukin (IL)-8 as major cytokines. Aesculetin inhibited alveolar epithelial induction of the mesenchymal markers in mCM-exposed/IL-8-loaded A549 cells (≈47–51% inhibition), while epithelial markers were induced in aesculetin-treated cells subject to mCM/IL-8 (≈1.5–2.3-fold induction). Aesculetin added to mCM-stimulated A549 cells abrogated the collagen production and alveolar epithelial CXC-chemokine receptor 2 (CXCR2) induction. The production of matrix metalloproteinase (MMP) proteins in mCM-loaded A549 cells was reduced by aesculetin (≈52% reduction), in parallel with its increase in tissue inhibitor of metalloproteinases (TIMP) proteins (≈1.8-fold increase). In addition, aesculetin enhanced epithelial induction of tight junction proteins in mCM-/IL-8-exposed cells (≈2.3–2.5-fold induction). The inhalation of polyhexamethylene guanidine (PHMG) in mice accompanied neutrophil predominance in bronchoalveolar lavage fluid (BALF) and macrophage infiltration in alveoli, which was inhibited by orally administrating aesculetin to mice. Treating aesculetin to mice alleviated PHMG-induced IL-8-mediated subepithelial fibrosis and airway barrier disruption. Taken together, aesculetin may antagonize pulmonary fibrosis and alveolar epithelial barrier disruption stimulated by the infiltration of monocyte-derived macrophages, which is typical of PHMG toxicity, involving interaction of IL-8 and CXCR2. Aesculetin maybe a promising agent counteracting macrophage-mediated inflammation-associated pulmonary disorders.
NR2F6 as a Prognostic Biomarker in HNSCC
Authors: Klapper Luise , Ribbat-Idel Julika , Kuppler Patrick , Paulsen Finn-Ole , Bruchhage Karl-Ludwig , Rades Dirk , Offermann Anne , Kirfel Jutta , Wollenberg Barbara , Idel Christian , Perner Sven
Published: August 2020

Head and neck squamous cell carcinoma (HNSCC)is the 6th most common cancer in humans worldwide and is associated with a poor prognosis for patients. NR2F6 has been identified as an immune checkpoint molecule in tumor-infiltrating T lymphocytes and is associated with a poor prognostic outcome in various cancers. The prognostic value of NR2F6 in HNSCC has not been described yet. We used a large, representative and clinically well-characterized cohort of 383 HNSCC patients, of which 22.4% developed a local recurrence. The NR2F6 expression was analyzed by using immunohistochemistry and was afterward correlated with clinical characteristics and clinicopathological features of HNSCC patients. Primary tumors from patients who develop a local recurrence have a higher NR2F6 expression than primary tumors which do not develop a local recurrence. Furthermore, a high NR2F6 expression is associated with poorer recurrence-free survival, although there is no correlation with overall survival. NR2F6 expression is independent of the T stage and UICC stage. NR2F6 might be a new prognostic biomarker for the early detection of local recurrences in HNSCC patients. Therefore, it may help to improve the recognition of patients who would benefit from more frequent follow-up examinations.
Vitamins and Uterine Fibroids: Current Data on Pathophysiology and Possible Clinical Relevance
Authors: Ciebiera Michał , Ali Mohamed , Zgliczyńska Magdalena , Skrzypczak Maciej , Al-Hendy Ayman
Published: August 2020

Uterine fibroid (UF) is the most common benign tumor pathology of the female reproductive organs. UFs constitute the main reason for a hysterectomy and hospitalization due to gynecological conditions. UFs consist of uterine smooth muscle immersed in a large amount of extracellular matrix (ECM). Genetic studies have demonstrated that UFs are monoclonal tumors originating from the myometrial stem cells that have underwent specific molecular changes to tumor initiating stem cells which proliferate and differentiate later under the influence of steroid hormones. There is growing interest in the role of micronutrients, for example, vitamins, in UFs. This article is a comprehensive review of publications regarding the available data concerning the role of vitamins in the biology and management of UFs. In summary, the results showed that some vitamins are important in the biology and pathophysiology of UFs. For example, vitamins A and D deserve particular attention following studies of their influence on the treatment of UF tumors. Vitamins B3, C, and E have not been as widely studied as the abovementioned vitamins. However, more research could reveal their potential role in UF biology.
Goat’s Milk Intake Prevents Obesity, Hepatic Steatosis and Insulin Resistance in Mice Fed A High-Fat Diet by Reducing Inflammatory Markers and Increasing Energy Expenditure and Mitochondrial Content in Skeletal Muscle
Authors: Delgadillo-Puga Claudia , Noriega Lilia G. , Morales-Romero Aurora M. , Nieto-Camacho Antonio , Granados-Portillo Omar , Rodríguez-López Leonardo A. , Alemán Gabriela , Furuzawa-Carballeda Janette , Tovar Armando R. , Cisneros-Zevallos Luis , Torre-Villalvazo Ivan
Published: August 2020

Goat’s milk is a rich source of bioactive compounds (peptides, conjugated linoleic acid, short chain fatty acids, monounsaturated and polyunsaturated fatty acids, polyphenols such as phytoestrogens and minerals among others) that exert important health benefits. However, goat’s milk composition depends on the type of food provided to the animal and thus, the abundance of bioactive compounds in milk depends on the dietary sources of the goat feed. The metabolic impact of goat milk rich in bioactive compounds during metabolic challenges such as a high-fat (HF) diet has not been explored. Thus, we evaluated the effect of milk from goats fed a conventional diet, a conventional diet supplemented with 30% Acacia farnesiana (AF) pods or grazing on metabolic alterations in mice fed a HF diet. Interestingly, the incorporation of goat’s milk in the diet decreased body weight and body fat mass, improved glucose tolerance, prevented adipose tissue hypertrophy and hepatic steatosis in mice fed a HF diet. These effects were associated with an increase in energy expenditure, augmented oxidative fibers in skeletal muscle, and reduced inflammatory markers. Consequently, goat’s milk can be considered a non-pharmacologic strategy to improve the metabolic alterations induced by a HF diet. Using the body surface area normalization method gave a conversion equivalent daily human intake dose of 1.4 to 2.8 glasses (250 mL per glass/day) of fresh goat milk for an adult of 60 kg, which can be used as reference for future clinical studies.
Social Fear Memory Requires Two Stages of Protein Synthesis in Mice
Authors: Kornhuber Johannes , Zoicas Iulia
Published: August 2020

It is well known that long-term consolidation of newly acquired information, including information related to social fear, require de novo protein synthesis. However, the temporal dynamics of protein synthesis during the consolidation of social fear memories is unclear. To address this question, mice received a single systemic injection with the protein synthesis inhibitor, anisomycin, at different time-points before or after social fear conditioning (SFC), and memory was assessed 24 h later. We showed that anisomycin impaired the consolidation of social fear memories in a time-point-dependent manner. Mice that received anisomycin 20 min before, immediately after, 6 h, or 8 h after SFC showed reduced expression of social fear, indicating impaired social fear memory, whereas anisomycin caused no effects when administered 4 h after SFC. These results suggest that consolidation of social fear memories requires two stages of protein synthesis: (1) an initial stage starting during or immediately after SFC, and (2) a second stage starting around 6 h after SFC and lasting for at least 5 h.
Function of Platelet Glycosphingolipid Microdomains/Lipid Rafts
Authors: Komatsuya Keisuke , Kaneko Kei , Kasahara Kohji
Published: August 2020

Lipid rafts are dynamic assemblies of glycosphingolipids, sphingomyelin, cholesterol, and specific proteins which are stabilized into platforms involved in the regulation of vital cellular processes. The rafts at the cell surface play important functions in signal transduction. Recent reports have demonstrated that lipid rafts are spatially and compositionally heterogeneous in the single-cell membrane. In this review, we summarize our recent data on living platelets using two specific probes of raft components: lysenin as a probe of sphingomyelin-rich rafts and BCθ as a probe of cholesterol-rich rafts. Sphingomyelin-rich rafts that are spatially and functionally distinct from the cholesterol-rich rafts were found at spreading platelets. Fibrin is translocated to sphingomyelin-rich rafts and platelet sphingomyelin-rich rafts act as platforms where extracellular fibrin and intracellular actomyosin join to promote clot retraction. On the other hand, the collagen receptor glycoprotein VI is known to be translocated to cholesterol-rich rafts during platelet adhesion to collagen. Furthermore, the functional roles of platelet glycosphingolipids and platelet raft-binding proteins including G protein-coupled receptors, stomatin, prohibitin, flotillin, and HflK/C-domain protein family, tetraspanin family, and calcium channels are discussed.
Comprehensive Analysis of Applicability Domains of QSPR Models for Chemical Reactions
Authors: Rakhimbekova Assima , Madzhidov Timur I. , Nugmanov Ramil I. , Gimadiev Timur R. , Baskin Igor I. , Varnek Alexandre
Published: August 2020

Nowadays, the problem of the model’s applicability domain (AD) definition is an active research topic in chemoinformatics. Although many various AD definitions for the models predicting properties of molecules (Quantitative Structure-Activity/Property Relationship (QSAR/QSPR) models) were described in the literature, no one for chemical reactions (Quantitative Reaction-Property Relationships (QRPR)) has been reported to date. The point is that a chemical reaction is a much more complex object than an individual molecule, and its yield, thermodynamic and kinetic characteristics depend not only on the structures of reactants and products but also on experimental conditions. The QRPR models’ performance largely depends on the way that chemical transformation is encoded. In this study, various AD definition methods extensively used in QSAR/QSPR studies of individual molecules, as well as several novel approaches suggested in this work for reactions, were benchmarked on several reaction datasets. The ability to exclude wrong reaction types, increase coverage, improve the model performance and detect Y-outliers were tested. As a result, several “best” AD definitions for the QRPR models predicting reaction characteristics have been revealed and tested on a previously published external dataset with a clear AD definition problem.
Expression and Role of Biosynthetic, Transporter, Receptor, and Responsive Genes for Auxin Signaling during Clubroot Disease Development
Authors: Robin Arif Hasan Khan , Saha Gopal , Laila Rawnak , Park Jong-In , Kim Hoy-Taek , Nou Ill-Sup
Published: August 2020

Auxins play a pivotal role in clubroot development caused by the obligate biotroph Plasmodiophora brassicae. In this study, we investigated the pattern of expression of 23 genes related to auxin biosynthesis, reception, and transport in Chinese cabbage (Brassica rapa) after inoculation with P. brassicae. The predicted proteins identified, based on the 23 selected auxin-related genes, were from protein kinase, receptor kinase, auxin responsive, auxin efflux carrier, transcriptional regulator, and the auxin-repressed protein family. These proteins differed in amino acids residue, molecular weights, isoelectric points, chromosomal location, and subcellular localization. Leaf and root tissues showed dynamic and organ-specific variation in expression of auxin-related genes. The BrGH3.3 gene, involved in auxin signaling, exhibited 84.4-fold increase in expression in root tissues compared to leaf tissues as an average of all samples. This gene accounted for 4.8-, 2.6-, and 5.1-fold higher expression at 3, 14, and 28 days post inoculation (dpi) in the inoculated root tissues compared to mock-treated roots. BrNIT1, an auxin signaling gene, and BrPIN1, an auxin transporter, were remarkably induced during both cortex infection at 14 dpi and gall formation at 28 dpi. BrDCK1, an auxin receptor, was upregulated during cortex infection at 14 dpi. The BrLAX1 gene, associated with root hair development, was induced at 1 dpi in infected roots, indicating its importance in primary infection. More interestingly, a significantly higher expression of BrARP1, an auxin-repressed gene, at both the primary and secondary phases of infection indicated a dynamic response of the host plant towards its resistance against P. brassicae. The results of this study improve our current understanding of the role of auxin-related genes in clubroot disease development.
Optical and Mechanical Properties of Highly Translucent Dental Zirconia
Authors: Kim Hee-Kyung
Published: August 2020

The aim was to evaluate the translucency, opalescence, and fluorescence of highly translucent zirconia, lithium disilicate, and bovine teeth. One mm-thick specimens of five monolithic zirconia systems, two glass-ceramics, and bovine enamel/dentin were investigated. A spectrophotometer (Ci7600) was used to measure the CIELab color coordinates, and the translucency and opalescence values were obtained. For evaluating the fluorescence emission, the differences in spectral reflectance by the UV component of illumination were calculated. The microstructures of ceramic specimens were examined with a scanning electron microscope and the chemical compositions were determined with an X-ray fluorescence spectrometer. Mechanical properties were appraised with three-point bending strength, indentation fracture toughness, and Vickers hardness. Data were analyzed using a one-way ANOVA, followed by Tukey’s multiple comparison test (α = 0.05). A higher yttria content (5 mol%) significantly improved the translucency of zirconia ceramics, while they were less translucent than lithium disilicate (p < 0.05). Lowering the alumina content below 0.05 wt.% enhanced the translucency (p < 0.05), but a small amount of alumina was still required to obtain full densification. 0.05 wt.% Fe was used to increase the chroma of zirconia specimens without compromising their mechanical properties. The Er-containing zirconia specimen showed a maximal fluorescence emission at 430 nm. The degree of opalescence was affected by the microstructures of ceramic materials. The microstructure, incorporation of a secondary phase, and sintering behavior can have a strong impact on the final mechanical and optical properties of dental ceramics. Addition of small amounts of metal oxides can affect the translucency, opalescence or fluorescence qualities of zirconia
Botulinum Toxin-Chitosan Nanoparticles Prevent Arrhythmia in Experimental Rat Models
Authors: Sergeevichev David , Fomenko Vladislav , Strelnikov Artem , Dokuchaeva Anna , Vasilieva Maria , Chepeleva Elena , Rusakova Yanina , Artemenko Sergey , Romanov Alexander , Salakhutdinov Nariman , Chernyavskiy Alexander
Published: August 2020

Several experimental studies have recently demonstrated that temporary autonomic block using botulinum toxin (BoNT/A1) might be a novel option for the treatment of atrial fibrillation. However, the assessment of antiarrhythmic properties of BoNT has so far been limited, relying exclusively on vagal stimulation and rapid atrial pacing models. The present study examined the antiarrhythmic effect of specially formulated BoNT/A1-chitosan nanoparticles (BTN) in calcium chloride-, barium chloride- and electrically induced arrhythmia rat models. BTN enhanced the effect of BoNT/A1. Subepicardial injection of BTN resulted in a significant antiarrhythmic effect in investigated rat models. BTN formulation antagonizes arrhythmia induced by the activation of Ca, K and Na channels.
Study on Characteristics of Electromagnetic Coil Used in MEMS Safety and Arming Device
Authors: Sun Yi , Lou Wenzhong , Feng Hengzhen , Zhao Yuecen
Published: August 2020

Traditional silicon-based micro-electro-mechanical system (MEMS) safety and arming devices, such as electro-thermal and electrostatically driven MEMS safety and arming devices, experience problems of high insecurity and require high voltage drive. For the current electromagnetic drive mode, the electromagnetic drive device is too large to be integrated. In order to address this problem, we present a new micro electromagnetically driven MEMS safety and arming device, in which the electromagnetic coil is small in size, with a large electromagnetic force. We firstly designed and calculated the geometric structure of the electromagnetic coil, and analyzed the model using COMSOL multiphysics field simulation software. The resulting error between the theoretical calculation and the simulation of the mechanical and electrical properties of the electromagnetic coil was less than 2% under the same size. We then carried out a parametric simulation of the electromagnetic coil, and combined it with the actual processing capacity to obtain the optimized structure of the electromagnetic coil. Finally, the electromagnetic coil was processed by deep silicon etching and the MEMS casting process. The actual electromagnetic force of the electromagnetic coil was measured on a micro-mechanical test system, compared with the simulation, and the comparison results were analyzed.
Primary Amebic Meningoencephalitis Related to Groundwater in Costa Rica: Diagnostic Confirmation of Three Cases and Environmental Investigation
Authors: Retana Moreira Lissette , Zamora Rojas Leidy , Grijalba Murillo Muriel , Molina Castro Silvia Elena , Abrahams Sandí Elizabeth
Published: August 2020

During the first trimester of 2020, the Ministry of Health of Costa Rica reported the first three cases of primary amebic meningoencephalitis (PAM). In two cases, laboratory personnel of the hospitals preliminarily identified amoeboid forms in cerebrospinal fluid (CSF) samples. For the molecular confirmation of species, CSF samples were sent to our laboratory. We carried out microscopic analyses and exflagellation assays. Besides, samples were cultured in 2% casein hydrolysate medium and in non-nutrient agar plates supplemented with Escherichia coli. Finally, PCR and sequencing were employed for the molecular diagnosis and species identification. In all cases, the presence of Naegleria fowleri was confirmed. An environmental investigation to identify the possible infection sources was also performed. Water samples from hot springs and groundwater from an artisan well were collected and after filtration and culture in non-nutrient agar plates supplemented with E. coli, thermotolerance and exflagellation assays were carried out. For the positive samples, PCR and sequencing were performed, confirming the presence of N. fowleri in several water samples. The report of these cases and the possible association with hot springs has had a significant impact on the population and health authorities of Costa Rica.
Deep Transfer Learning for Time Series Data Based on Sensor Modality Classification
Authors: Li Frédéric , Shirahama Kimiaki , Nisar Muhammad Adeel , Huang Xinyu , Grzegorzek Marcin
Published: August 2020

The scarcity of labelled time-series data can hinder a proper training of deep learning models. This is especially relevant for the growing field of ubiquitous computing, where data coming from wearable devices have to be analysed using pattern recognition techniques to provide meaningful applications. To address this problem, we propose a transfer learning method based on attributing sensor modality labels to a large amount of time-series data collected from various application fields. Using these data, our method firstly trains a Deep Neural Network (DNN) that can learn general characteristics of time-series data, then transfers it to another DNN designed to solve a specific target problem. In addition, we propose a general architecture that can adapt the transferred DNN regardless of the sensors used in the target field making our approach in particular suitable for multichannel data. We test our method for two ubiquitous computing problems—Human Activity Recognition (HAR) and Emotion Recognition (ER)—and compare it a baseline training the DNN without using transfer learning. For HAR, we also introduce a new dataset, Cognitive Village-MSBand (CogAge), which contains data for 61 atomic activities acquired from three wearable devices (smartphone, smartwatch, and smartglasses). Our results show that our transfer learning approach outperforms the baseline for both HAR and ER.
Validation of an IMU Suit for Military-Based Tasks
Authors: Mavor Matthew P. , Ross Gwyneth B. , Clouthier Allison L. , Karakolis Thomas , Graham Ryan B.
Published: August 2020

Investigating the effects of load carriage on military soldiers using optical motion capture is challenging. However, inertial measurement units (IMUs) provide a promising alternative. Our purpose was to compare optical motion capture with an Xsens IMU system in terms of movement reconstruction using principal component analysis (PCA) using correlation coefficients and joint kinematics using root mean squared error (RMSE). Eighteen civilians performed military-type movements while their motion was recorded using both optical and IMU-based systems. Tasks included walking, running, and transitioning between running, kneeling, and prone positions. PCA was applied to both the optical and virtual IMU markers, and the correlations between the principal component (PC) scores were assessed. Full-body joint angles were calculated and compared using RMSE between optical markers, IMU data, and virtual markers generated from IMU data with and without coordinate system alignment. There was good agreement in movement reconstruction using PCA; the average correlation coefficient was 0.81 ± 0.14. RMSE values between the optical markers and IMU data for flexion-extension were less than 9°, and 15° for the lower and upper limbs, respectively, across all tasks. The underlying biomechanical model and associated coordinate systems appear to influence RMSE values the most. The IMU system appears appropriate for capturing and reconstructing full-body motion variability for military-based movements.
Vision Measurement of Gear Pitting Under Different Scenes by Deep Mask R-CNN
Authors: Xi Dejun , Qin Yi , Wang Yangyang
Published: August 2020

To accurately and quantitatively detect the gear pitting of different levels on the actual site, this paper studies a new vision measurement approach based on a tunable vision detection platform and the mask region-based convolutional neural network (Mask R-CNN). The shooting angle can be properly set according to the specification of the target gear. With the obtained sample set of 1500 gear pitting images, an optimized deep Mask R-CNN was designed for the quantitative measurement of gear pitting. The effective tooth surface and pitting was firstly and simultaneously recognized, then they were segmented to calculate the pitting area ratio. Considering three situations of multi-level pitting, multi-illumination, and multi-angle, several indexes were used to evaluate detection and segmentation results of deep Mask R-CNN. Experimental results show that the proposed method has higher measurement accuracy than the traditional method based on image processing, thus it has significant practical potential.
Time-Domain Investigation of Switchable Filter Wide-Band Antenna for Microwave Breast Imaging
Authors: Haider Amir , Rahman MuhibUr , Naghshvarianjahromi Mahdi , Kim Hyung Seok
Published: August 2020

This paper investigates the time-domain performance of a switchable filter impulse radio ultra-wideband (IR-UWB) antenna for microwave breast imaging applications. A miniaturized CPW-fed integrated filter antenna with switchable performance in the range of the Worldwide Interoperability for Microwave Access (WiMAX) and Wireless Local Area Network (WLAN) bands could operate well within a 3.0 to 11 GHz frequency range. The time-domain performance of the filter antenna was investigated in comparison to that of the designed reference wideband antenna. By comparing both antennas’ time-domain characteristics, it was seen that the switchable filter antenna had good time-domain resolution along with the frequency-domain operation. Additionally, the time-domain investigation revealed that the switchable filter wide-band antenna performed similarly to the reference wide band antenna. This antenna was also utilized for a tumor detection application, and it was seen that the switchable filter wide-band antenna could detect a miniaturized irregularly shaped tumor easily, which is quite promising. Such an antenna with a good time-domain resolution and tumor detection capability will be a good candidate and will find potential applications in microwave breast imaging.
Validity Evaluation Method Based on Data Driving for On-Line Monitoring Data of Transformer under DC-Bias
Authors: He Yuanda , Zhou Qi , Lin Sheng , Zhao Liping
Published: August 2020

The DC-bias monitoring device of a transformer is easily affected by external noise interference, equipment aging, and communication failure, which makes it difficult to guarantee the validity of monitoring data and causes great problems for future data analysis. For this reason, this paper proposes a validity evaluation method based on data driving for the on-line monitoring data of a transformer under DC-bias. First, the variation rule and threshold range of monitoring data for neutral point DC, vibration, and noise of the transformer under different working conditions are obtained through statistical analysis. Then, the data validity criterion of DC bias monitoring data is proposed to achieve a comprehensive evaluation of data validity based on data threshold, continuity, impact, and correlation. In addition, case studies are carried out on the real measured data of the DC bias magnetic monitoring system of a regional power grid by using this evaluation method. The results show that the proposed method can systematically and comprehensively evaluate the validity of the DC bias monitoring data and can judge whether the monitoring device fails to a certain extent.
Pediatric Chronic Rhinosinusitis: Unmet Needs
Authors: Hopp Russell
Published: August 2020

Pediatric chronic rhinosinusitis (CRS) remains an elusive diagnostic medical condition, largely based on imperfect diagnostic criteria, lack of controlled studies of therapy, lack of measure for resolution, and lack of information of pediatric sinus microbiome dysbiosis. The true prevalence of pediatric CRS is unknown, and symptoms often over-lap with other diagnoses. We review the unmet needs in pediatric CRS, to highlight potential research opportunities to improve understanding and therapy of the disease process.
Financial and Non-Financial Practices Driving Sustainable Firm Performance: Evidence from Banking Sector of Developing Countries
Authors: Asghar Bilal , Wasim Ahmad , Qazi Usama , Rasool Azfar
Published: August 2020

Since independence, state-owned enterprises in Pakistan have been struggling for performance enhancement. The goal of sustainable performance is still unachievable. Therefore, the paper evaluates sustainable corporate performance based on financial, social, and environmental performance areas. The organizational restructuring framework for sustainable performance enhancement is developed on software PLS-SEM. The financial and economic performance (FEP) was evaluated through financial reports and surveys; however, social and environmental performances (SEP) were quantified through survey questionnaires for seven performance areas with multiple sub factors, based on Weisbord’s six box model. The study time period in focus is 2011 to 2015. Data was collected from 517 employees of 19 public, private, and privatized banks of Pakistan. The results demonstrate that the total effect of FEP and SEP is much stronger (t-value = 7.619) than the individual direct impact of FEP (t-value = 5.189) on sustainable firm performance (SFP). This is a clear indication of the mediating role of SEP for SFP evaluation. Furthermore, FEP depends on significant indicators include net assets, total deposits, profit before tax, and earnings per share of total deposits with outer loadings, which are given as 0.995, 0.992, 0.978, and 0.954, respectively. Moreover, SEP depends on indicators, i.e., reward policies, redefining organizational purpose, coordination mechanism among employees, and supervisor relationships, with correlations of 0.864, 0.849, 0.805, and 0.761, respectively. The framework will assist in the enhancement of the performance of economically unviable public and loss-making privatized entities.
Adaptation of the Management Model of Internationalization Processes in the Development of Railway Transport Activities
Authors: Jarašūnienė Aldona , Sinkevičius Gintaras , Čižiūnienė Kristina , Čereška Audrius
Published: August 2020

The paper analyzes the importance of the system of management of internationalization processes in developing railway transport. The article also highlights the synergistic effect of a multilevel management model in internationalization processes. The main purpose of the paper was to establish a new model of management of internationalization processes in the frame of railway transport development, presenting a related management model. The article analyzes possibilities of integration of the national railway transport sector into global railway networks and provides a model for the management of internationalization processes in developing railway transport. The developed model was approved in an expert survey conducted in application of a questionnaire method. The goal of the expert evaluation was to ascertain the suitability of the theoretical management model of internationalization processes. Expert evaluation consisting of a questionnaire, the evaluation of concordance of expert opinions, and the results obtained were used in the analysis. The research led to the development of a new railway transport model using the “alliance + cluster” system, which is based on the management of internationalization processes. Many theoretical sources on internationalization processes were analyzed with the aim to adapt main advantages of the internationalization theory in the field of development of railway transport and its prospects for further research.
The Implementation Of Football Sports Extracurricular Activities In Junior High School 25 Padang
Authors: Apriyano Berto , Wiranata Rendra , Atradinal Atradinal
Published: August 2020

The purpose of the study is to know the implementation of extracurricular sports football activities in junior high Country 25 fields, include the variable quality of coaches, infrastructure, and motivated athletes. The type of this research is descriptive. The entire research population athletes/students who follow football-playing ie 17 people. With a drawing of samples is done with the Total sampling, where all athletes/students extracurricular activities that follow the sport of football. Research data collection instrument using the question form by using the Guttman scale. Data were analyzed with descriptive statistics. Using the formula P = f / n x 100. Data collection of the entire variable, namely the quality of coaches, infrastructure, the motivation of athletes does with question form deployment techniques. Data analysis is a descriptive analysis and conducting interviews and observation. Data analysis of the results of the implementation of extracurricular sports football activities in junior high Country Meadow is at 25 classifications.
The Effect Of Plyometrics Training Method On Sabit Kicks
Authors: Melico Afdalia , Emral Emral , Alnedral Alnedral
Published: August 2020

The problem in this research is the low skill Crescent Kick Athlete Skills Pencak Silat Anak Nagari South Coastal District that affect the appearance and cause decreased performance. The problem is likely to occur due to a lack of training methods used precisely by the coach. This study aims to reveal the effect of the method of the Skills Training Plyometrics Crescent kick. The research is a quasi-experiment. The population in this study are all athletes Pencak Silat Anak Nagari South Coastal District totaling 50 people. The sampling technique used a purposive sampling method, the total of samples used as many as 25 people. Data collected by Skills test crescent kick by kicking to the target 20 times kick. Data were analyzed using a t-test or a different test mean. Results of research and analysis of the data showed that the method Plyometrics exercises a significant influence on the athlete's skills Sabit kick Pencak Silat Anak Nagari South Coastal District. Based on the analysis, it was found t = 30.2> table = 1.71 with an average increase was 12.08.
The Biomechanic Of Bridge Up Analysis
Authors: Lusiana
Published: August 2020

Sport is a physical activity to maintain physic fitness and health. Physical fitness can be obtained with correct physical activity, one of which is by doing gymnastic movements. Bridge up is one of the movements in gymnastics on the floor with a supine body shape or posture which rests on both hands and legs with knees bent. The correct movement is a movement that is in accordance with the anatomy and physiology of the human body, coupled with a mechanical study of efficient movement. In terms of biomechanics, the mechanical laws of motion are: 1) center of gravity, 2) balance and 3) force. It takes coordination between balance, flexibility and good strength to be able to make movement bridge up. Efforts to avoid mistakes that can result in injury can be done by applying the principles of proper training and adequate stretching. In the learning process, a teacher/lecturer must pay attention to the condition of students by providing step by step exercises or a series of movements from simple to complex.
High School Student Physical Fitness Profile
Authors: Wahyudi Ade , Bafirman Bafirman
Published: August 2020

This study is to determine the physical fitness profile of high school students of Negeri 1 Sungai Penuh City. The sample in this study were 193 people consisting of 113 female students and 80 male students. Data were collected using the TKJI instrument and then classified according to the TKJI norm. The data obtained is then processed using a percentage formula. The results showed that there were differences in average physical fitness of male students 11.16 while female students 10.71.