bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined Rough Sets and Rule-Based Expert System to Support Environmentally Oriented Sandwich Pallet Loading Problem
Authors: Sawicki Piotr , Sawicka Hanna , Karkula Marek , Zajda Krzysztof
Published: January 2025

A sandwich pallet loading problem represents a significant challenge in the logistics of fast-moving consumer goods (FMCG), requiring optimisation of load units (LUs) arrangements to minimise their number in transportation and warehousing processes, leading to an environmental responsibility of organisations. This study introduces an innovative approach combining Dominance-Based Rough Set Theory (DRST) with a rule-based expert system to improve the efficiency of the pallet loading and to provide sustainable development. Key criteria and attributes for the LU assessment, such as weight, height, and fragility, are defined. DRST is utilised to classify these units, leveraging its capability to handle imprecise and vague information. The rule-based system ensures an optimal arrangement of LUs by considering critical control parameters, thereby reducing LU numbers and mitigating the environmental impact of logistics operations, as measured by energy consumption. The proposed approach is validated using real-world data from the FMCG distribution company. Results demonstrate that integrating DRST with an expert system improves decision-making consistency and significantly reduces the number of LUs. This study shows a way to increase the level of environmental responsibility of the organisation by cutting energy consumption and delivering economic and social benefits through fewer shipments. For example, the approach reduces energy consumption for a customer order delivery by 40%, from 0.60 to 0.36 (kWh/pskm).
Key Ecological and Cultural Characteristics of Homestead Windbreak Forest Landscapes in Okinawa, Japan
Authors: Chen Bixia , Minor Jesse
Published: January 2025

This study investigates the Fukugi (Garcinia subelliptica) windbreak landscapes on Iriomote Island through case studies in two of its oldest villages, Sonai and Hoshitate. These windbreak forests, integral to the cultural landscape of Okinawa, offer both ecological and socio-economic benefits. Using field measurements and surveys, the research analyzes the distribution, growth patterns, and historical significance of Fukugi groves within the village setting and compares naturally regenerated forests with those planted by humans. The findings underscore the importance of Fukugi trees in promoting sustainable rural landscapes, where they dominate the local ecosystem of rural settlements. Fukugi windbreak landscapes in Okinawa are characterized by trees encircling homes on all four sides, distinguishing them from homestead windbreaks found in other regions of Japan. Surveys from the two villages suggest that the original homestead windbreak forests in the Yaeyama region contain a diverse mix of tree species, with Fukugi and Calophyllum inophyllum being predominant, along with other useful species like Diospyros ferrea and Podocarpus macrophyllus. These species were selected not only for their windbreak capabilities but also for timber and home furniture production. The study’s findings on naturally regenerated Fukugi groups support the hypothesis that the cultivation of Fukugi as a windbreak species originated in the Yaeyama region and likely spread to other parts of Okinawa, influencing both the ecological and cultural evolution of the region’s landscapes.
Effects of Forest Land Mulching on the Soil CO2 Emission Rate of Phyllostachys violascens Forests
Authors: Shen Zhan , Zha Dongping , Zu Xinglan , Shi Jianmin , Li Zuyao , Chu Shuangshuang
Published: January 2025

This study investigates the dynamics of soil CO2 emissions during the cover period of Phyllostachys violascens and the impact of different cover measures, aiming to provide references for reducing the environmental effects of bamboo cover. An L27 (913) orthogonal experimental design was employed, setting the following variables: (1) heating materials: chicken manure, straw cake, and wheat ash; (2) thickness of husk layer: 15 cm, 25 cm, and 35 cm; (3) soil moisture levels before covering: moisture to 10 cm, 15 cm, and 20 cm. The soil CO2 emission rate showed a unimodal curve, with a significant overall increase during the cover period. Throughout the entire cover period, the average soil CO2 emission rate (25.39 μmol·m−2·s−1) was 5.1 times higher than that of the uncovered Lei bamboo forest (5.02 μmol·m−2·s−1) during the same period. Thicker husk layers (25 cm and 35 cm) corresponded to higher soil CO2 emission rates, with significant differences noted among the thicknesses. When the soil was moist to 10 cm, the CO2 emission rate was highest (62.51 μmol·m−2·s−1); moisture to 15 cm and 20 cm resulted in significantly lower emission rates. Chicken manure produced the highest peak CO2 emissions in the third week, at 70.64 μmol·m−2·s−1, while straw cake and wheat ash reached their peaks in the fifth week, at 66.56 μmol·m−2·s−1 and 57.58 μmol·m−2·s−1, respectively. The interactions between the three factors (heating materials, husk layer thickness, and moisture levels) significantly affected the soil CO2 emission rates. By optimally configuring these factors, CO2 emissions can be regulated. This study recommends using wheat ash or straw cake as heating materials, combined with a 25 cm husk layer thickness, and moistening the soil to 15 cm before covering. This approach effectively reduces the peak and total soil CO2 emissions while ensuring suitable soil temperatures for the growth of bamboo shoots in spring. This research provides a scientific basis for the environmental management of bamboo forests, aiding in the optimization of covering measures to achieve low-carbon and sustainable bamboo management.
Depth-Dependent Impacts of Long-Term Vegetation Restoration on Soil Carbon Stability and C/N Stoichiometry in Subtropical Plantations
Authors: Song Xianwei , Yang Lu , Nong Haiqin , Lyu Sidan , Wang Jingyuan
Published: January 2025

Vegetation restoration has played a significant role in preventing soil erosion and enhancing soil carbon sequestration in the hilly regions of subtropical China. However, under long-term vegetation restoration, it remains unclear how soil organic carbon and nitrogen components synergistically change and whether their dynamics are consistent across different soil depths. This study investigates the soils of four major vegetation types in subtropical hilly regions: enclosed Masson pine forest (EMP), Masson pine forest (MP), slash pine forest (SP), and mixed broadleaf–conifer forest (MBC). Soil organic carbon (SOC) components, nitrogen components, and physicochemical properties were analyzed across different soil depths (0–100 cm) under these vegetation restoration types. Results showed that forest type significantly influenced SOC stability. The MBC exhibited the highest mineral-associated organic carbon (MAOC) content, indicating the highest SOC stability. Both the MBC and SP forests sequestered more iron-aluminum-bound organic carbon (FeOC) and less Ca-associated organic carbon (CaOC) compared to the other forest types. The dominance of FeOC over CaOC in these acidic soils highlights the critical role of iron-aluminum oxides in SOCSOC stabilization. Vegetation restoration significantly influenced the relationship between SOC and nitrogen components in the topsoil (0–20 cm), but this effect diminished in the subsoil (>20 cm). This study confirmed the depth-dependent impacts of long-term vegetation restoration on soil carbon stability and C/N stoichiometry in subtropical plantations. Targeted vegetation restoration strategies can improve SOC stability in subtropical plantations.
Any Sugar with That? Assessment of Dissolved Sucrose as Supplementary Feed in Nursery Rearing of Juvenile Bivalves
Authors: Jordan Andy , Skelton Bradley , Mugica Maria , Jeffs Andrew
Published: January 2025

The costly production of live microalgal feed prevents the inclusion of an extended nursery phase in bivalve aquaculture. One method of feeding juvenile bivalves that has received minimal attention is the use of dissolved nutrients to reduce the reliance on live microalgae as a sole feed input. This study aimed to determine whether dissolved sucrose could work as a supplement to live microalgae. Two different concentrations of dissolved sucrose (i.e., 100 μg mL−1 and 1 mg mL−1) were each provided daily for 2 h and 4 h to juvenile Greenshell™ mussels as a supplement to a diet of live microalgae. The growth and survival of the mussels were measured over three weeks. All combinations of sucrose concentrations and exposures improved the growth of spat compared with the control without sucrose. However, the best-performing spat were provided with a concentration of 1 mg mL−1 of dissolved sucrose for an exposure time of 4 h, which induced 57% greater spat growth daily compared with the control diet. The mussel spat supplemented with dissolved sucrose also accumulated greater carbohydrate content compared with those in the control treatment, indicating they were in greater nutritional condition. This demonstration that dissolved sucrose can significantly improve the growth and nutritional composition of mussel spat over periods as short as 2 h shows promise for the commercial application of sucrose as low-cost supplementary feed in bivalve nurseries.
An Aptamer Sensor Based on Alendronic Acid-Modified Upconversion Nanoparticles Combined with Magnetic Separation for Rapid and Sensitive Detection of Thiamethoxam
Authors: Huang Qian , Han Lu , Ma Hui , Lan Weijie , Tu Kang , Peng Jing , Su Jing , Pan Leiqing
Published: January 2025

The widespread use of thiamethoxam has led to pesticide residues that have sparked global concerns regarding ecological and human health risks. A pressing requirement exists for a detection method that is both swift and sensitive. Herein, we introduced an innovative fluorescence biosensor constructed from alendronic acid (ADA)-modified upconversion nanoparticles (UCNPs) linked with magnetic nanoparticles (MNPs) via aptamer recognition for the detection of thiamethoxam. Through base pairing, thiamethoxam-specific aptamer-functionalized MNPs (apt-MNPs) were integrated with complementary DNA-functionalized UCNPs (cDNA-UCNPs) to create the MNPs@UCNPs fluorescence biosensor. Thiamethoxam specifically attached to apt-MNPs, leading to their separation from cDNA-UCNPs, which in turn led to a reduction in fluorescence intensity at 544 nm following separation by an external magnetic field. The change in fluorescence intensity (ΔI) was directly correlated with the concentration of thiamethoxam, enabling the quantitative analysis of the pesticide. With optimized detection parameters, the biosensor was capable of quantifying thiamethoxam within a concentration span of 0.4–102.4 ng·mL−1, and it achieved a detection limit as minute as 0.08 ng·mL−1. Moreover, leveraging the swift magnetic concentration properties of MNPs, the assay duration could be abbreviated to 25 min. The research exhibited a swift and precise sensing platform that yielded promising results in samples of cucumber, cabbage, and apple.
Characterization of Anthocyanins Including Acetylated Glycosides from Highbush Blueberry (Vaccinium corymbosum L.) Cultivated in Korea Based on UPLC-DAD-QToF/MS and UPLC-Qtrap-MS/MS
Authors: Kim Ju Hyung , Kwon Ryeong Ha , Kim So Ah , Na Hyemin , Cho Jeong-Yong , Kim Heon-Woong
Published: January 2025

In this study, anthocyanin glycosides from nine cultivars of highbush blueberries grown in Korea were characterized using UPLC-DAD-QToF/MS and UPLC-Qtrap-MS/MS. A total of twenty-two derivatives were identified, consisting of mono-glycosides and acetyl-glycosides attached to aglycones, such as cyanidin, peonidin, delphinidin, petunidin, and malvidin. Among them, seven acetylated glycosides were tentatively determined by comparing the related authentic standards and previous reports and presented mass fragmentation, in which the acetyl group remained as the form attached to the sugar without de-esterification in positive ionization mode. The mid-season cultivar ‘New Hanover’ showed the highest total anthocyanin content (1011.7 mg/100 g dry weight) with predominant malvidin and delphinidin glycosides. Particularly, the ‘Patriot’ (early season) recorded the highest proportion of acetylated glycosides (19.7%). Multivariate analysis showed a distinct separation between early and mid-seasons with Draper. Especially, delphinidin 3-O-galactoside (VIP = 1.94) was identified as a marker for mid-season, and malvidin 3-O-glucoside (VIP = 1.79) was identified as a marker for early season. These comprehensive anthocyanin profiles of Korean blueberries will serve as fundamental data for breeding superior cultivars, evaluating and developing related products as well as clinical and metabolomic research.
The Moderating Role of Social Capital Between Parenting Stress and Mental Health and Well-Being Among Working Mothers in China
Authors: He Langjie , Mai Zheyuan , Guan Xin , Cai Pengpeng , Li Xuhong , Feng Juxiong , Hung Suetlin
Published: January 2025

Rapid economic growth has transformed social and family structure globally, including in China, over the past few decades. With women’s engagement in the workforce, the dual demand of paid and domestic work has become a major source of stress, particularly among mothers. Working mothers face unique challenges of family obligations blended with contemporary values of women’s career aspirations. Methods: This study recruited 189 participants. This study employed quantitative methods to investigate the relationship between parenting stress and mental health, and the moderating role of social capital, among working mothers in Zhaoqing, Guangdong province of China. Results: The findings suggested a high level of parenting stress among Chinese working mothers. Their stress is associated with depressive symptoms. What should be noted is that social capital acts as a moderator between their stress and depressive symptoms. Discussion: This study reveals the buffering effect of social capital against parenting stress and depression in Chinese working mothers, with an emphasis on the importance of workplace flexibility and social support systems in addressing work–family conflicts. The study enriches the study of the buffer effect of social capital on mental health. This knowledge can inform the development of targeted interventions and support systems to improve working mothers’ overall well-being.
Stress and Burnout Among Anesthesia Technologists, Technicians, and Trainees: A Cross-Sectional Study in a Tertiary Hospital in Saudi Arabia
Authors: Alsabani Mohmad H. , Aljohani Fay , Alkathiri Ghaid Rakan , Alkhonain Judy Saad , Aljuhani Lama , Alanazi Shahad , Olayan Lafi H. , Aljuhani Turki , Alenezi Faraj K. , Al Harbi Mohammed K.
Published: January 2025

Background/Objectives: Occupational burnout poses a significant burden to healthcare personnel, institutions, and service users. Anesthesia technologists and technicians work in the shadow of the perioperative team, and a lack of attention to anesthesia support personnel may pose a significant risk to their wellbeing and the quality of care delivered. To date, only a few studies have investigated the prevalence of burnout among anesthesia technologists and technicians worldwide and in Saudi Arabia. Thus, the aim of this cross-sectional study was to assess the prevalence and contributing factors to burnout among anesthesia technologists and technicians in a single tertiary hospital in Saudi Arabia. Methods: The study utilized the Maslach Burnout Inventory-Human Services Survey (MBI-HSS) to assess burnout and a 10-point scale to assess stress levels. The MBI-HSS inventory consists of three subscales: emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA). Univariate and multivariate linear regression analyses were used to identify correlates of each burnout subscale. Gender was included in the multivariable regression analysis in addition to significant variables from univariable analysis. Results: A total of 89 participants completed the survey. Based on each subscale of the MBI-HSS, more than 60% of the participants reported high to moderate EE, and more than half reported high to moderate DP. For PA, only 25.8% of participants reported low PA. We found that age (β = −0.58, 95% CI: −0.95, −0.20; p = 0.003) and stress (β = 3.3, 95% CI: 2.1, 4.5; p < 0.001) were independently associated with EE. In addition, night shift (β = 3.3, 95% CI: 0.44, 6.1; p = 0.024) and stress (β = 0.73, 95% CI: 0.13, 1.3; p = 0.017) were independently associated with DP. Independent factors for PA were identified including night shifts (β = 6.6, 95% CI: 1.4, 12; p = 0.014) and stress levels (β = −1.3, 95% CI: −2.4, −0.12; p = 0.03). Conclusions: This research underscores the alarmingly high prevalence of burnout and the strong link between elevated EE and DP rates and workplace stress, emphasizing the necessity to identify and mitigate these stressors. It is therefore crucial to evaluate the effectiveness of the current wellbeing and mental health initiatives and programs in Saudi Arabia to ensure that they address evolving challenges and the overall mental health of healthcare personnel.
Genomic Characterization and Functional Validation of Six cis-Regulatory Sequences in Medicinal Plant Andrographis paniculata
Authors: Lv Xingbin , Yang Hua , Hu Yufang , Liang Qi , Tian Shuyun , Yang Lang , Huang Mingkun , Zhang Ling , Xu Yanqin
Published: January 2025

Plant cis-regulatory sequences (CRSs) are essential for gene expression and transcriptional regulation in plants. With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the 35S promoter, to avoid some negative effects caused by exogenous DNA sequences. In this study, we provided a comprehensive analysis of the published genomic data of Andrographis paniculata and identified six candidate ApACRs. Based on the transient assays, two candidate CRSs (ApACR1 and ApACR2) demonstrated high transcriptional activities comparable to those of the 35S promoter in several plant species, in contrast to others with lower activities, suggesting their further potential applications in plant biotechnology and genetic engineering.
GSTP1 and GSTO1 Variant Alleles Affect Susceptibility to Helicobacter pylori Infection and Severity of Helicobacter pylori-Associated Clinical Manifestations
Authors: Pantic Ivana , Lugonja Sofija , Jerotic Djurdja , Pljesa-Ercegovac Marija , Matic Marija , Bakovic Nikola , Vojnovic Marko , Simic Tatjana , Milovanovic Tamara , Savic-Radojevic Ana
Published: January 2025

Considering the mutual relationship between redox disbalance and inflammation in Helicobacter pylori (HP) infection, we aimed to evaluate whether the polymorphisms in antioxidant glutathione transferases genes (GSTP1 rs1695, GSTP1rs1138272, GSTO1 rs4925 and GSTO2 rs156697) modify susceptibility to HP infection, as well as the severity of HP-associated gastric manifestation development. Therefore, GST gene polymorphisms were determined via the appropriate PCR in 101 HP-positive and 107 HP-negative patients. Our results show that carriers of the GSTP1*G/G variant genotype (rs1695) or at least one GSTP1*T variant allele (rs1138272) were more prone to the development of HP-positive gastritis compared with reference allele carriers (OR = 3.21, 95%CI = 1.15–8.91, p = 0.025 and OR = 2.31, 95%CI = 1.14–4.89, p = 0.021, respectively), which was confirmed by haplotype analysis. HP-positive carriers of the GSTO1*A variant allele showed increased risk of developing gastric atrophy and precancerous gastric lesions compared with the reference one (OR = 2.49, 95%CI:1.04–5.96, p = 0.04 and OR = 2.98, 95%CI = 1.21–7.34, p = 0.018, respectively). HP-positive carriers of the GSTO2*G variant allele were less prone to developing moderate/severe inflammatory infiltration (OR = 0.35, 95%CI = 1.04–5.96, p = 0.04), whereas the GSTP1*T variant allele was significantly associated with active inflammation (OR = 4.09, 95%CI = 1.04–5.96, p = 0.042). In conclusion, antioxidant GST genetic propensity seems to have an important impact on both acute and chronic forms of HP infection.
Optimization and Validation of an Ultra-Performance Liquid Chromatography with Quadrupole Detector Mass Spectrometry Quantification Method for the Simultaneous Detection of Tazarotene and Tazarotenic Acid in Porcine Skin: An In Vitro Study
Authors: Hamzehpour Helena , Hauksson Kristófer H. , Jónsson Helgi , Gizurarson Sveinbjorn , Snorradottir Bergthora S.
Published: January 2025

Exploring tazarotene, a third-generation retinoid for potential hand osteoarthritis treatment, this study presents the development and validation of an ultra-performance liquid chromatography with quadrupole detector mass spectrometry (UPLC-QDa) method for the simultaneous quantification of tazarotene and tazarotenic acid, its active metabolite, in porcine skin. Method development involved a design-of-experiments approach for chromatographic optimization of gradient steepness, organic solvent volume, column temperature, capillary voltage, flow rate, and cone voltage. Central composite orthogonal design was used to optimize peak area, peak width, retention time, and resolution. Validation was performed in accordance with U.S. Food and Drug Administration guidelines. The method was linear over the concentration range of 0.4–18,750 ng/mL for tazarotene and 13.3–12,500 ng/mL for tazarotenic acid, with r2 values of ≥0.99. Chromatographic analysis demonstrated acceptable accuracy and precision (<15%), and stability tests confirmed the analytes’ stability under various conditions. This validated method offers a reliable and accurate approach for the simultaneous analysis of tazarotene and tazarotenic acid, facilitating further research into their therapeutic applications for hand osteoarthritis.
Differential Activity and Expression of Proteasome in Seminiferous Epithelium During Mouse Spermatogenesis
Authors: Zapata-Carmona Héctor , Díaz Emilce Silvina , Morales Patricio , Jara Marco
Published: January 2025

Proteasome-mediated protein degradation is essential for maintaining cellular homeostasis, particularly during spermatogenesis, where extensive cellular transformations, such as spermatid differentiation, require precise protein turnover. A key player in this process is the ubiquitin–proteasome system (UPS). This study aimed to investigate proteasome enzymatic activity at different stages of the spermatogenic cycle within the seminiferous tubules of mice and explore the regulatory mechanisms that influence its proteolytic function. Specifically, we assessed the trypsin-like, chymotrypsin-like, and peptidyl-glutamyl-peptide-hydrolyzing (PGPH) activities of the proteasome. Additionally, we examined the expression of catalytic and structural subunits of the 20S core, the assembly of the 20S core with regulatory complexes, and the phosphorylation status of proteasome subunits in various segments of the seminiferous tubules. Our findings demonstrated distinct patterns of proteasomal enzymatic activity in the analyzed segments. While the expression levels of structural and catalytic subunits of the 20S core remained consistent, significant differences were detected in the assembly of the 20S core, the expression of regulatory complexes, and the phosphorylation of proteasome subunits mediated by protein kinase A. These results indicate that proteasomal activity is finely regulated through multiple mechanisms depending on the specific stage of the seminiferous epithelial cycle, highlighting the complexity of proteostasis during spermatogenesis.
Evaluation of 3D-Printed Microfluidic Structures for Use in AML-Specific Biomarker Detection of PML::RARA
Authors: Emde Benedikt , Niehaus Karsten , Tickenbrock Lara
Published: January 2025

An obstacle for many microfluidic developments is the fabrication of its structures, which is often complex, time-consuming, and expensive. Additive manufacturing can help to reduce these barriers. This study investigated whether the results of a microfluidic assay for the detection of the promyelocytic leukemia (PML)-retinoic acid receptor α (RARα) fusion protein (PML::RARA), and thus for the differential diagnosis of acute promyelocytic leukemia (APL), could be transferred from borosilicate glass microfluidic structures to additively manufactured fluidics. Digital light processing (DLP) and stereolithography (SLA) printers as well as different photopolymerizable methacrylate-based resins were tested for fabrication of the fluidics. To assess suitability, both print resolution and various physical properties, serializability, biocompatibility, and functionalization with biological molecules were analyzed. The results show that additively manufactured microfluidics are suitable for application in leukemia diagnostics. This was demonstrated by transferring the microfluidic sandwich enzyme-linked immunosorbent assay (ELISA) for PML::RARA onto the surface of magnetic microparticles from a glass structure to three-dimensional (3D)-printed parts. A comparison with conventional glass microstructures suggests lower sensitivity but highlights the potential of additive manufacturing for prototyping microfluidics. This may contribute to the wider use of microfluidics in biotechnological or medical applications.
Gboxin Induced Apoptosis and Ferroptosis of Cervical Cancer Cells by Promoting Autophagy-Mediated Inhibition of Nrf2 Signaling Under Low-Glucose Conditions
Authors: Liu Wei , Lu Junlin , Li Jiarui , Wang Lu , Chen Yao , Wu Yulun , Zhang Ziying , Zhang Jingying , Gao Feng , Jia Chaoran , Bao Yongli , Yang Xiaoguang , Song Zhenbo
Published: January 2025

Cervical cancer poses a substantial threat to women’s health, underscoring the necessity for effective therapeutic agents with low toxicity that specifically target cancer cells. As cancer progresses, increased glucose consumption causes glucose scarcity in the tumor microenvironment (TME). Consequently, it is imperative to identify pharmacological agents capable of effectively killing cancer cells under conditions of low glucose availability within the TME. Previous studies showed that Gboxin, a small molecule, inhibited glioblastoma (GBM) growth by targeting ATP synthase without harming normal cells. However, its effects and mechanisms in cervical cancer cells in low-glucose environments are not clear. This study indicates that Gboxin notably enhanced autophagy, apoptosis, and ferroptosis in cervical cells under low-glucose conditions without significantly affecting cell survival under normal conditions. Further analysis revealed that Gboxin inhibited the activity of complex V and the production of ATP, concurrently leading to a reduction in mitochondrial membrane potential and the mtDNA copy number under low-glucose culture conditions. Moreover, Gboxin inhibited tumor growth under nutrient deprivation conditions in vivo. A mechanistic analysis revealed that Gboxin activated the AMPK signaling pathway by targeting mitochondrial complex V. Furthermore, increased AMPK activation subsequently promoted autophagy and reduced p62 protein levels. The decreased levels of p62 protein facilitated the degradation of Nrf2 by regulating the p62-Keap1-Nrf2 axis, thereby diminishing the antioxidant capacity of cervical cancer cells, ultimately leading to the induction of apoptosis and ferroptosis. This study provides a better theoretical basis for exploring Gboxin as a potential drug for cervical cancer treatment.
Evidence Suggesting That Alzheimer’s Disease May Be a Transmissible Disorder
Authors: Saw Genevieve , Yi Ling-Xiao , Tan Eng King , Zhou Zhi Dong
Published: January 2025

Alzheimer’s disease (AD) is characterised by progressive neurodegeneration with the formation of amyloid beta (Aβ) plaques and neurofibrillary tau tangles in the brain parenchyma. The causes of AD have been attributed to a combination of age-related changes within the brain as well as genetic, environmental and lifestyle factors. However, a recent study by Banerjee et al. highlights the possibility that AD may be a transmissible disease and that iatrogenic AD could be environmentally acquired, similar to iatrogenic Creutzfeldt–Jakob disease (iCJD). The study reports that contaminated Aβ in cadaver-derived pituitary growth hormone (c-hGH) therapy, which patients received during childhood inoculation, may accidentally transmit into their brains, triggering neurodegeneration and AD onset in older age. Furthermore, corroborating evidence from various animal model studies and human case reports suggests that AD can be potentially transmissible.
Luminescence Properties of Hoechst 33258 in Polyvinyl Alcohol Films
Authors: Lee Bong , Jablonska Agnieszka , Pham Danh , Sagoo Rajveer , Gryczynski Zygmunt , Pham Trang Thien , Gryczynski Ignacy
Published: January 2025

We report a comprehensive investigation of the photophysical properties of Hoechst 33258 (HOE) embedded in polyvinyl alcohol (PVA) films. HOE displays a bright, highly polarized, blue fluorescence emission centered at 430 nm, indicating effective immobilization within the polymer matrix of PVA. Its fluorescence quantum yield is notably high (~0.74), as determined relative to a quinine sulfate standard. In addition, we observed that HOE-doped PVA films exhibit room temperature phosphorescence (RTP) that remains visible for several seconds after UV excitation ceases. The slightly negative phosphorescence anisotropy implies that the triplet–singlet radiative transition is orthogonal to the singlet–singlet transition governing fluorescence. Notably, we observed that direct triplet-state excitation at longer wavelengths (beyond the primary absorption band) produces highly polarized RTP. We believe this possibility of direct triplet-state excitation opens new avenues for studying RTP in polymer-immobilized molecules.
A Comparative Kinetic and Thermodynamic Adsorption Study of Methylene Blue and Its Analogue Dye on Filter Paper
Authors: Bogyor Andrea , Csavdari Alexandra Ana , Lovász Tamás , Bitay Enikő
Published: January 2025

A comparative adsorption study was carried out for methylene blue (MB) and its 3,7-bis(N,N-(2-hydroxyethyl)amino)-phenothiazinium dye analog (MBI). Batch experiments employed aqueous solutions and commercial filter paper. Out of seven kinetic models tested by means of four quality statistical indicators, the pseudo-second-order, the double-exponential, and the bi-linear Weber–Morris equations were best fits. For both dyes, the process was described as a succession of two diffusion-controlled steps. The Freundlich isotherm was chosen from 11 models describing a variety of mechanism assumptions. Physisorption was considered responsible for the dye removal from liquid. Adsorption of MB is thermodynamically favored, whereas that of MBI is sterically hindered. Both processes are exothermic and exhibit reduced randomness at the S-L interface. The paper was found suitable for retaining MB but served rather filtration/purification purposes for MBI.
Post, Predict, and Rank: Exploring the Relationship Between Social Media Strategy and Higher Education Institution Rankings
Authors: Rocha Bruna , Figueira Álvaro
Published: January 2025

In today’s competitive higher education sector, institutions increasingly rely on international rankings to secure financial resources, attract top-tier talent, and elevate their global reputation. Simultaneously, these universities have expanded their presence on social media, utilizing sophisticated posting strategies to disseminate information and boost recognition and engagement. This study examines the relationship between higher education institutions’ (HEIs’) rankings and their social media posting strategies. We gathered and analyzed publications from 18 HEIs featured in a consolidated ranking system, examining various features of their social media posts. To better understand these strategies, we categorized the posts into five predefined topics—engagement, research, image, society, and education. This categorization, combined with Long Short-Term Memory (LSTM) and a Random Forest (RF) algorithm, was utilized to predict social media output in the last five days of each month, achieving successful results. This paper further explores how variations in these social media strategies correlate with the rankings of HEIs. Our findings suggest a nuanced interaction between social media engagement and the perceived prestige of HEIs.
Physiology-Versus Angiography-Guided Complete Coronary Revascularization in STEMI Patients with Multivessel Disease: A Network Meta-Analysis
Authors: Martino Giovanni , Quarta Rossella , Greco Francesco , Spaccarotella Carmen , Indolfi Ciro , Curcio Antonio , Polimeni Alberto
Published: January 2025

Background: In patients with ST-segment elevation myocardial infarction (STEMI) and multivessel disease (MVD), complete revascularization (CR) is recommended over culprit-only PCI to reduce adverse cardiovascular outcomes. However, the optimal strategy for CR, whether angiography (Angio)-guided or physiology-guided, remains uncertain. Methods: This network meta-analysis included 14 randomized controlled trials (RCTs) with 11,568 patients to compare the efficacy of angio-guided CR, physiology-guided CR, and culprit-only PCI in reducing major adverse cardiovascular events (MACE), all-cause mortality, recurrent myocardial infarction (MI), cardiovascular (CV) death, and unplanned revascularization. The frequentist and Bayesian approaches were applied to assess the effectiveness of each strategy. Results: The pairwise meta-analysis showed that angio-guided CR showed superior efficacy, significantly reducing MACE (OR = 0.44; 95% CI: 0.37–0.52), recurrent myocardial infarction, and unplanned revascularization compared to culprit-only PCI. Physiology-guided CR also reduced MACE (OR = 0.64, 95% CI: 0.45–0.91) and unplanned revascularization. The network metanalysis showed that CV death was lower in the physiology-guided CR group (OR 0.56; 95% CI 0.25–1.05), suggesting a protective effect, but the difference did not reach statistical significance. Furthermore, physiology-guided CR was not significantly better than angio-guided CR in most outcomes. Conclusions: Angio-guided CR appears to provide the best overall outcomes for patients with STEMI and MVD, outperforming physiology-guided CR in most endpoints. Further large-scale trials are needed to clarify the relative efficacy of angio-guided CR and physiology-guided CR in this patient population.
The Impact of Fibular Fixation Method on Pilon Fracture Healing
Authors: Perugini Anthony , Hyland Scott , Iandoli James , Hill Zachary , Peabody John , DeGenova Daniel , Faherty Mallory , Taylor Benjamin
Published: January 2025

Background: Pilon fractures are associated with high-energy injuries, and there is presently much debate as to optimal fixation strategies and timing of intervention. There is little evidence comparing the type of fibular fixation during pilon fracture fixation. The purpose of this study was to compare fibular fixation methods in complex pilon injuries as it relates to pilon union rates and development of post-traumatic arthritis. Methods: This was a retrospective review from an urban Level 1 trauma center from January 2009 to May 2019, including patients age ≥ 18 who sustained a pilon fracture with an associated fibula fracture. Patients were allocated into one of three groups based on fibular fracture treated with plating, intramedullary device, or no fixation. Radiographic analysis was performed postoperatively and at final follow up to evaluate for tibial or fibular nonunion, malunion, talocrural angle, and ankle Kellgren–Lawrence grade. Results: Of the 107 patients in this study, 42 underwent surgical fixation of their fibular fracture. There were no differences with respect to tibial or fibular union rates amongst the three groups. Furthermore, there were no differences in the presence of radiographic ankle arthritis at final follow up. However, Kellgren–Lawrence arthritis grading did appear to be a more severe grade in patients who did not undergo fibular fixation (p = 0.001). Conclusions: Fibular intramedullary fixation does not appear to influence tibial or fibular nonunion rates as compared to plating in complex pilon injuries.
Long-Term Survivor with Paraneoplastic Cerebellar Ataxia and Small-Cell Lung Cancer
Authors: Tsoukalas Konstantinos , Ntanasis-Stathopoulos Ioannis , Andrikopoulou Angeliki , Tzartos John S. , Dimopoulos Meletios A. , Gavriatopoulou Maria
Published: January 2025

Background/Objectives: Paraneoplastic cerebellar degeneration (PCD) is an inflammatory autoimmune process caused by onconeural antibodies directed against cerebellar Purkinje cells. In most cases, prognosis is poor as disease progression leads to pancerebellar dysfunction and permanent neurological damage. Through this case report, we aim to highlight the clinical presentation, diagnostic process, and therapeutic implications associated with PCD secondary to SCLC. Methods: Herein, we present the case of a 57-year-old patient diagnosed with PCD who presented with progressive limb ataxia and impaired mobility. CT scans and EBUS (endobronchial ultrasound) bronchoscopy established the diagnosis of limited-stage small-cell lung cancer (SCLC) of the right lung with marked lymphadenopathy. Results: Anti-CV2/CRMP5 and anti-SOX1 autoantibodies were identified in the serum that confirmed the diagnosis of PCD related to SCLC. A total of six cycles of chemotherapy with carboplatin and etoposide resulted in rapid clinical improvement and complete response of the disease. The patient remains in remission six years after the initial diagnosis with no neurological deficits. Conclusions: The prognosis of PCD greatly depends on early detection and management of the underlying malignancy. Despite the poor prognosis, early diagnosis and prompt initiation of chemotherapy may offer a great survival benefit in these patients.
Long-Term Clinical Outcomes of Transalveolar Maxillary Sinus Floor Elevation with Rotatory Instruments: An 8-Year Follow-Up Prospective Clinical Study
Authors: Jiménez-Guerra Álvaro , Velasco-Ortega Eugenio , Matos-Garrido Nuno , Ortiz-García Iván , Moreno-Muñoz Jesús , Núñez-Márquez Enrique , Rondón-Romero José-Luis , Kewalramani Naresh , Salgado-Peralvo Ángel-Orión , Monsalve-Guil Loreto
Published: January 2025

Background: Transalveolar sinus floor elevation (TSFE) is a surgical technique for the placement of dental implants in patients with reduced height of the maxillary posterior alveolar bone. This study aims to demonstrate the clinical outcomes of TSFE using the minimal invasive sinus elevation (MISE) technique in partially and totally edentulous maxillary patients. Methods: This prospective clinical study followed STROBE guidelines. TSFE was performed using the MISE technique with the simultaneous placement of implants. Dental implants were loaded at 6 months. Maxillary vertical bone gain was measured by CBCT, and marginal bone loss was assessed by periapical radiographs. Results: Ninety-one patients, with a mean age of 62.1 ± 11.8 years, were treated with TSFE and the placement of 107 implants, with a mean follow-up of 96.2 ± 11.7 months. An increase of 4.3 ± 0.4 mm in bone height was achieved, with a dental implant cumulative survival rate of 97.2%. Peri-implantitis was observed in 9.3% of implants, and membrane perforation occurred in 7.7% of cases. Technical complications were noted in 5.5% of patients. Conclusions: Within the limitations of this clinical study, it can be concluded that the MISE technique is a successful protocol for the placement of implants in the posterior maxilla with reduced height of the alveolar ridge, with a rate of biological and prosthetic complications below 10% over an average follow-up period of 8 years.
Hereditary Pancreatic Cancer: Advances in Genetic Testing, Early Detection Strategies, and Personalized Management
Authors: Blanco Abad Carmen , Gomila Pons Paula , Campos Ramírez Sara , Álvarez Alejandro María , Torres Ramón María Irene , Miramar Gallart María Dolores , Izquierdo Álvarez Silvia , Polo Marques Eduardo , Pazo Cid Roberto
Published: January 2025

Background: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with a five-year survival rate of approximately 13% for advanced stages. While the majority of PDAC cases are sporadic, a significant subset is attributable to hereditary and familial predispositions, accounting for approximately 25% of cases. This article synthesizes recent advancements in the understanding, detection, and management of hereditary pancreatic cancer (PC). Results: Our review highlights the critical role of genetic testing (GT) in identifying high-risk individuals (HRIs), with germline pathogenic variants (PVs) found in up to 20% of hereditary PDAC cases. Since the implementation of next-generation sequencing (NGS) panels in 2014, detection capabilities have been significantly enhanced. HRIs can be included in screening programs that facilitate the early detection of PDAC. Early detection strategies, including the use of microribonucleic acid (miRNAs) signatures and novel imaging techniques like hyperpolarized 13C-magnetic resonance spectroscopy (MRS) have shown promising results. The identification of germline pathogenic variants (PVs) or mutations in homologous recombination (HR) genes plays a predictive role in the response to various treatments, prolonging patient survival. Discussion: Universal germline testing for PDAC, as recommended by the National Comprehensive Cancer Network (NCCN), is now a standard practice, facilitating the identification of at-risk individuals and enabling targeted surveillance and intervention. Multidisciplinary management, integrating genetic counseling, imaging, and gastrointestinal services, is essential for optimizing outcomes. Conclusions: Advances in genetic testing and biomarker research are transforming the landscape of hereditary PC management. Early detection and personalized treatment strategies are pivotal in improving survival rates. Ongoing multi-institutional research efforts are crucial for validating biomarkers and developing preventive measures, ultimately aiming to reduce the burden of this aggressive cancer.
Prevalence of Knee Pain and Its Relation to Depression, Anxiety, and Health-Related Quality of Life Among Maintenance Hemodialysis Patients
Authors: Tharwat Samar , Nagy Eman , Elsayed Abdelrahman Mohammed , Salem Karem Mohamed , Salah Ahmed M. , Mohamed Sherin Zohdy , Nassar Mohammed Kamal
Published: January 2025

Background/Objectives: Knee pain in hemodialysis (HD) patients might affect health-related quality of life (HRQoL) and may be related to anxiety and depressive symptoms. The aim of this study was to assess the prevalence of knee pain in chronic HD patients and to determine its relationship with anxiety, depression, and HRQoL, Methods: This multicenter cross-sectional study was carried out on chronic HD patients. Sociodemographic, clinical, and therapeutic data were collected. The Knee Pain Screening Tool (KNEST) was used to screen for knee pain. Patients with knee pain were instructed to complete the visual analog scale (VAS) for pain and the Western Ontario and McMaster Universities Arthritis Index (WOMAC). The patients also completed an Arabic-language version of the Hospital Anxiety and Depression Scale (HADS) and the Kidney Disease Quality of Life-36 (KDQOL-36™) questionnaire. Results: This study included 271 chronic HD patients; the median age was 51 (IQR 21) years, and most of them were males (59%). Of them, 158 had knee pain. Those with knee pain were more likely to have anxiety compared to those without (p = 0.002) and significantly lower scores on the symptom/problem (p = 0.03) and burden of kidney disease domains (p = 0.047) and the physical health (p < 0.001) and mental health components (p = 0.001). Furthermore, those with moderate to severe knee pain were more likely to experience anxiety (p = 0.001) and depression (p = 0.005) and have a lower physical health composite (PHC) than those with mild knee pain (p = 0.046). Conclusions: HD patients have a significant prevalence of knee pain that is usually associated with anxiety and leads to worse HRQoL than those without knee pain.
Video Head Impulse Test in Children—A Systematic Review of Literature
Authors: Dasgupta Soumit , Mukherjee Aditya Lal , Crunkhorn Rosa , Dawabah Safaa , Aslier Nesibe Gul , Ratnayake Sudhira , Manzari Leonardo
Published: January 2025

Background and Objectives: The video head impulse test is a landmark in vestibular diagnostic methods to assess the high-frequency semicircular canal system. This test is well established in the adult population with immense research since its discovery. The usefulness and feasibility of the test in children is not very well defined, as research has been limited. This systematic review investigated and analysed the existing evidence regarding the test. The objectives were to derive meaningful inferences in terms of the feasibility, implementation, and normative vestibulo-ocular reflex (VOR gain) in normal children and in children with vestibular hypofunction. Methods: Research repositories were searched with keywords, along with inclusion and exclusion criteria, to select publications that investigated the vHIT in both a normative population of children as well as in pathological cohorts. The average normal VOR gain was then calculated in all semicircular canals for both the normal and the vestibular hypofunction groups. For the case–control studies, a meta-analysis was performed to assess the heterogeneity and pooled effect sizes. Results and Discussion: The review analysed 26 articles that included six case–control studies fulfilling the study selection criteria, out of more than 6000 articles that have been published on the vHIT. The described technique suggested 10–15 head impulses at 100–200°/s head velocity and 10–20° displacement fixating on a wall target 1 to 1.5 m away. The average VOR gain in the lateral semicircular canals combining all studies was 0.96 +/− 0.07; in anterior semicircular canals, it was 0.89 +/− 0.13, and for posterior semicircular canals, it was 0.9 +/− 0.12. The normal VOR gains measured with individual equipment (ICS Impulse, EyeSeeCam and Synapsys) in the lateral semicircular canals were largely similar (p > 0.05 when ICS Impulse and EyeSeeCam were compared). The pooled effect size in the control group was 1, and the heterogeneity was high. It was also observed that implementing the test is different from that in adults and requires considerable practice with children, factoring in the issue of peripheral and central vestibular maturation. Special considerations were suggested in terms of the pupillary calibration, goggle fitting, and slippage and play techniques. Conclusions: The vHIT as a diagnostic test is possible in children with important caveats, practice, and knowledge regarding a developing vestibular system. It yields significantly meaningful inferences about high-frequency semicircular canal function in children. Adult norms should not be extrapolated in children, as the VOR gain is different in children.