bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological Activity of Peptide Fraction Derived from Hermetia illucens L. (Diptera: Stratiomyidae) Larvae Haemolymph on Gastric Cancer Cells
Authors: Rinaldi Roberta , Laurino Simona , Salvia Rosanna , Russi Sabino , De Stefano Federica , Galasso Rocco , Sgambato Alessandro , Scieuzo Carmen , Falco Geppino , Falabella Patrizia
Published: February 2025

Gastric cancer (GC) is one of the leading causes of cancer-related mortality worldwide, characterised by poor prognosis and limited responsiveness to chemotherapy. There is a need for new and more effective anticancer agents. Antimicrobial peptides (AMPs) represent a promising class of biomolecules for this purpose. Naturally occurring in the innate immune system, these peptides can also exert cytotoxic effects against cancer cells, earning them the designation of “anticancer peptides” (ACPs). They have the potential to be a viable support for current chemotherapy schedules due to their selectivity against cancer cells and minor propensity to induce chemoresistance in cells. Insects are an excellent source of AMPs. Among them, due to its ability to thrive in hostile and microorganism-rich environments, we isolated a peptide fraction from Hermetia illucens L. (Diptera: Stratiomyidae) haemolymph to evaluate a possible anticancer activity. We tested Peptide Fractions (PFs) against AGS and KATO III gastric cancer cell lines. Data obtained indicated that PFs, especially those resulting from Escherichia coli and Micrococcus flavus infection (to boost immune response), were able to inhibit tumour cell growth by inducing apoptosis or cell cycle arrest in a cell line-specific manner. These results support further investigation into the use of antimicrobial peptides produced from insects as possible anticancer agents.
The Natural Antherea pernyi Sericin Protein Suppresses Gastric Cancer Formation by Inhibiting Cell Proliferation and Inducing Cell Apoptosis
Authors: Wei Qiong-Qiong , Xiao Yu , Wu Qiao , Jing Cai , Dong Zhan-Qi , Chen Peng , Pan Min-Hui
Published: February 2025

Sericin, a natural macromolecular protein and the main component of silkworm cocoons, exhibits biocompatibility, excellent mechanical properties, and biodegradability. Previous research has confirmed that the sericin protein possesses anticancer properties. Gastric cancer (GC) poses a serious hazard to human health, with a low rate of early diagnosis and a poor prognosis. Investigating the safety and effectiveness of drugs for their used in treatment is imperative. In this study, we confirmed that Antherea pernyi sericin (APS) inhibited the proliferation, migration, and clonal formation of GC cells and caused apoptosis in the cells by regulating the expression of Bcl2 and Bax. Moreover, our data show that APS did not exhibit significant toxicity in normal gastric mucosal cells and mice. Furthermore, the results show that APS suppressed the proliferation of cisplatin-resistant GC cells and promoted cellular apoptosis; however, it had no synergistic effects with cisplatin. All the results indicated that APS exhibits antitumor activity against GC and is a prospective medicinal agent for the clinical treatment of GC, with minimal toxicity and adverse side effects. This research can provide a theoretical basis for sericin in the field of tumor treatment, especially for the application of natural macromolecular polypeptide drugs.
Salt-Resilient Cowpeas: Early Identification Through Growth Parameters and Gene Expression at Germination Stage
Authors: Afonso Patrícia , Castro Isaura , Carvalho Márcia
Published: February 2025

Soil salinity is one of the most severe impacts of climate change, negatively affecting plant growth and development. Seed germination and seedling emergence are among the most critical stages susceptible to salt stress, making it important to explore them to identify the most resilient accessions for crop yield improvement. Cowpea (Vigna unguiculata L. Walp.) is an important crop due to its ability to fix atmospheric nitrogen, improving soil health, and its high protein content. The main objectives of this study were to screen salt-resilient cowpea accessions from a worldwide collection and to evaluate cowpea responses to salt stress at germination stage through gene expression analysis. A total of 40 cowpea accessions from sixteen different countries were subjected to two treatments: control (water) and salt stress (150 mM NaCl solution). The seeds germinated, and the seedlings grew for ten days. The germination and growth parameters and lipid peroxidation quantification were determined. The results revealed significant differences in all parameters among accessions and treatments. A high variation in salt responses was detected among accessions, allowing the selection of five accessions (Co_2, Co_4, Co_21, Co_30, Co_31) as resilient to salt stress at germination stage. Subsequently, two salt stress-related genes (DREB2 and VuEXO) were evaluated through qPCR, revealing genotype-dependent regulation. These results provide valuable insights for the early selection of salt-resilient cowpea accessions, which may be considered for the development of improved and new varieties in the future.
Cannabis sativa L. Leaf Oil Displays Cardiovascular Protective Effects in Hypertensive Rats
Authors: Khamseekaew Juthamas , Duangjinda Monchai , Maneesai Putcharawipa , Labjit Chanon , Rattanakanokchai Siwayu , Rongpan Sudarat , Pakdeechote Poungrat , Potue Prapassorn
Published: February 2025

Hemp (Cannabis sativa L.) leaf oil (HLO) contains several bioactive compounds such as phenolics, flavonoids, and quercetin. However, the effects of HLO on hypertensive conditions have not yet been investigated. This study investigated the cardiovascular protective effects of HLO in a nitric oxide (NO) synthase inhibitor-induced hypertensive rat model. Five weeks of HLO administration significantly prevented blood pressure elevation, improved cardiac function, and mitigated cardiac hypertrophy. Furthermore, HLO ameliorated vascular dysfunction by reducing sympathetic nerve stimulation-induced vasoconstriction, increasing endothelium-dependent vasorelaxation, as well as decreasing vascular wall thickness and vascular smooth muscle cell proliferation. HLO inhibited renin–angiotensin system (RAS) activation and downregulated angiotensin II type 1 (AT1) receptor and NADPH oxidase expression. Additionally, HLO normalized the circulating NO metabolites, decreased oxidative stress, and enhanced antioxidant status. These findings suggest that HLO protects against cardiovascular dysfunction and preserves its morphology. The mechanism of action might involve the suppression of RAS overactivity and oxidative stress through the Ang II/AT1 receptor/NOX2 pathway in NO-deficient hypertension.
Electrospinning Synthesis of Nano-Scale MgO Fibers and Their Methylene Blue Adsorption Efficiency
Authors: Ma Chunyang , He Hongxin , Li Huaxing , Cao Mengyu , Xia Fafeng
Published: February 2025

Water pollution from industrial dyes like methylene blue poses severe environmental and health risks, necessitating effective wastewater treatment methods. Among various adsorbents, MgO stands out due to its high surface area, tunable porosity, and superior adsorption capabilities. This research presents the preparation of nano-scale magnesium oxide (MgO) fibers using electrospinning, followed by calcination at temperatures of 300 °C, 400 °C, 500 °C, 600 °C, and 700 °C. The effects of calcination temperatures on MgO’s surface characteristics, microstructure, crystalline phases, and adsorption performance were investigated. SEM and TEM analyses revealed that fibers calcined at 500 °C possessed the most distinct porous structure, with a coarse surface and substantial pores, which enhanced adsorption properties. XRD analysis confirmed that the 500 °C calcined MgO fibers had the highest crystallinity, particularly the (200) crystal plane. Notably, BET surface area analysis confirmed the superior adsorption properties of these fibers, making them highly effective for wastewater treatment applications. Adsorption tests for methylene blue (MB) indicated that these fibers achieved a maximum dye removal efficiency of 52.52% and an adsorption capacity of 43.11 mg/g within 90 min. The adsorption process aligned with a quasi-second-order kinetic model (R2 = 0.9846) and fit the Langmuir isotherm (R2 = 0.991), indicating monolayer chemisorption. This study underscores the effectiveness of MgO fibers calcined at 500 °C, demonstrating enhanced adsorption characteristics that are beneficial for wastewater treatment applications.
Pertuzumab in Combination with Trastuzumab and Docetaxel as Adjuvant Doublet Therapy for HER2-Positive Breast Cancer: A Systematic Review
Authors: Ventura Ignacio , Salcedo Nerea Pinilla , Pérez-Bermejo Marcelino , Pérez-Murillo Javier , Tejeda-Adell Manuel , Tomás-Aguirre Francisco , Legidos-García María Ester , Murillo-Llorente María Teresa
Published: February 2025

Breast cancer is the most clinically relevant pathology of the mammary gland and is currently the most diagnosed malignant disease among women worldwide. In breast cancer prevention, it is important to consider that the risk of developing the disease is not the same for the entire population. This pathology presents heterogeneous clinical manifestations and the most common classification is related to the following hormonal receptors: estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), and triple-negative (TNBC). Currently, a new class of therapy is being used for cancer treatment: anti-body-drug conjugates. A bibliographic search was performed by establishing keywords and then combining them using Boolean operators OR and AND. Thus, the search equation was formulated according to the PICO search question to be used in the PubMed database. Results: Fifteen studies that met the established inclusion criteria were analyzed, and their methodological quality was assessed using the Joanna Briggs Institute approach, demonstrating high reliability in the results obtained, the analyzed studies focus on the combination of adjuvant Pertuzumab + Trastuzumab with chemotherapy for the treatment of HER2-positive breast cancer. Scientific evidence suggests that the combination of pertuzumab and trastuzumab not only improves the survival of patients with HER2-positive breast cancer but also provides a safe and flexible treatment option.
Research Progress on Anti-Inflammatory Mechanism of Inula cappa
Authors: Wu Ningning , Wang Siqi , Zhang Yuqian , Wang Siming
Published: February 2025

The incidence of various inflammatory diseases has remained high. Inula cappa is a kind of Chinese herbal medicine with a wide range of pharmacological uses and application value. It has anti-inflammatory, antibacterial, antioxidant, hepatoprotective and other pharmacological activities. The monomeric compounds that have been confirmed to have anti-inflammatory effects are luteolin, chrysoerilol, artemetin, chlorogenic acid, neochlorogenic acid, cryptchlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and 1,3-O-dicaffeoylquinic acid. This article introduces the relationship between Inula cappa and inflammation, the anti-inflammatory components of I. cappa, the modulation of each component on the inflammatory transduction signal pathway, and the TLR2/MyD88/NF-KB anti-inflammatory signaling pathway, providing a theoretical basis for anti-inflammatory research on and clinical medication using Inula cappa.
Unveiling Novel miRNA–mRNA Interactions and Their Prognostic Roles in Triple-Negative Breast Cancer: Insights into miR-210, miR-183, miR-21, and miR-181b
Authors: Xu Jiatong , Cai Xiaoxuan , Huang Junyang , Huang Hsi-Yuan , Wang Yong-Fei , Ji Xiang , Huang Yuxin , Ni Jie , Zuo Huali , Li Shangfu , Lin Yang-Chi-Dung , Huang Hsien-Da
Published: February 2025

Triple-negative breast cancer (TNBC) poses a major clinical challenge due to its aggressive progression and limited treatment options, making early diagnosis and prognosis critical. MicroRNAs (miRNAs) are crucial post-transcriptional regulators that influence gene expression. In this study, we unveil novel miRNA–mRNA interactions and introduce a prognostic model based on miRNA–target interaction (MTI), integrating miRNA–mRNA regulatory correlation inference and the machine learning method to effectively predict the survival outcomes in TNBC cohorts. Using this method, we identified four key miRNAs (miR-181b-5p, miR-21-5p, miR-210-3p, miR-183-5p) targeting eight downstream target genes, forming a novel regulatory network of 19 validated miRNA–mRNA pairs. A prognostic model constructed based on the top 10 significant MTI pairs using random forest combination effectively classified patient survival outcomes in both TCGA and independent dataset GSE19783 cohorts, demonstrating good predictive accuracy and valuable prognostic insights for TNBC patients. Further analysis uncovered a complex network of 71 coherent feed-forward loops involving transcription factors, miRNAs, and target genes, shedding light on the mechanisms driving TNBC progression. This study underscores the importance of considering regulatory networks in cancer prognosis and provides a foundation for new therapeutic strategies aimed at improving TNBC treatment outcomes.
Dexmedetomidine Reduces Presynaptic γ-Aminobutyric Acid Release and Prolongs Postsynaptic Responses in Layer 5 Pyramidal Neurons in the Primary Somatosensory Cortex of Mice
Authors: Tang Bo , Tang Jiali , Huang Yuguang
Published: February 2025

Dexmedetomidine (DEX) exhibits notable sedative, analgesic, and anesthetic-sparing properties. While growing evidence suggests these effects are linked to the modulation of γ-aminobutyric acid (GABA) system, the precise pre- and postsynaptic mechanisms of DEX action on cortical GABAergic signaling remain unclear. In this study, we applied whole-cell patch-clamp recording to investigate the impact of DEX on GABAergic transmission in layer 5 pyramidal neurons of the mouse primary somatosensory cortex. We recorded spontaneous inhibitory postsynaptic currents (sIPSCs), miniature IPSCs (mIPSCs), and evoked inhibitory postsynaptic potentials (eIPSPs) before and during DEX application. Our findings demonstrated that DEX reduced activity-dependent spontaneous GABAergic transmission, as evidenced by a decrease in sIPSC frequency, while mIPSC frequency was unaffected. eIPSPs were not significantly influenced by DEX either. Additionally, DEX prolonged the kinetics of both sIPSCs and mIPSCs, increasing the rise and decay times of sIPSCs and the decay time of mIPSCs. We proposed that DEX modulated cortical neuronal activity by limiting GABA release and altering GABAA receptor kinetics. Collectively, these results indicated that DEX modulated cortical GABAergic signaling at both presynaptic and postsynaptic sites, which likely underlined its sedative, analgesic, and anesthetic-sparing effects.
Clinical Importance of Amyloid Beta Implication in the Detection and Treatment of Alzheimer’s Disease
Authors: Pokrzyk Justyna , Kulczyńska-Przybik Agnieszka , Guzik-Makaruk Ewa , Winkel Izabela , Mroczko Barbara
Published: February 2025

The role of amyloid beta peptide (Aβ) in memory regulation has been a subject of substantial interest and debate in neuroscience, because of both physiological and clinical issues. Understanding the dual nature of Aβ in memory regulation is crucial for developing effective treatments for Alzheimer’s disease (AD). Moreover, accurate detection and quantification methods of Aβ isoforms have been tested for diagnostic purposes and therapeutic interventions. This review provides insight into the current knowledge about the methods of amyloid beta detection in vivo and in vitro by fluid tests and brain imaging methods (PET), which allow for preclinical recognition of the disease. Currently, the priority in the development of new therapies for Alzheimer’s disease has been given to potential changes in the progression of the disease. In light of increasing amounts of data, this review was focused on the diagnostic and therapeutic employment of amyloid beta in Alzheimer’s disease.
Exploring the Potential of Coumarin Derivatives on Serotonin Receptors 5-HT1A and 5HT2A
Authors: Ostrowska Kinga , Horosz Gabriela , Kruk Karolina , Sieroń Bartłomiej , Leśniak Anna , Czartoryska Zofia , Bujalska-Zadrożny Magdalena , Milenkovic Dejan , Trzaskowski Bartosz
Published: February 2025

A series of 2- and 3-methoxyphenylpiperazine derivatives in combination with a 2-hydroxypropoxy linker and coumarins containing various substituents was synthesized and evaluated for antidepressant-like activity. Microwave-assisted synthesis was used, and the structures of all compounds were confirmed by 1H, 13C NMR, and HRMS spectrometry. The affinity toward the 5-HT1A and 5-HT2A receptors was determined using radioligand binding assays and analyzed by molecular docking studies. Among the compounds evaluated, four demonstrated high affinity for the 5-HT1A receptor with the following Ki values: 5-(2-hydroxy-3-(4-(2-methoxyphenyl)piperazin-1-yl)propoxy)-4,7-dimethyl-2H-chromen-2-one (5) (90 nM), 6-acetyl-5-(2-hydroxy-3-(4-(2-methoxyphenyl)piperazin-1-yl)propoxy)-4,7-dimethyl-2H-chromen-2-one (7) (90 nM), 7-(2-hydroxy-3-(4-(3-methoxyphenyl)piperazin-1-yl) propoxy)-4-methyl-2H-chromen-2-one (10) (87 nM), and 8-acetyl-7-(2-hydroxy-3-(4-(2-methoxy phenyl)piperazin-1-yl)propoxy)-4-methyl-2H-chromen-2-one (11) (96 nM), and four demonstrated high affinity for the 5-HT2A receptor with the following Ki values: 6-acetyl-7-(2-hydroxy-3-(4-(3-methoxyphenyl)piperazin-1-yl)propoxy)-4-methyl-2H-chromen-2-one (2) (83 nM), 8-acetyl-7-(2-hydroxy-3-(4-(3-methoxyphenyl)piperazin-1-yl)propoxy)-4-methyl-2H-chromen-2-one (12) (67 nM), 7-(2-hydroxy-3-(4-(2-methoxyphenyl)piperazin-1-yl) propoxy)-2H-chromen-2-one (13) (18 nM), and 7-(2-hydroxy-3-(4-(3-methoxyphenyl)piperazin-1-yl)propoxy)-2H-chromen-2-one (14) (68 nM). In functional assays, 8-acetyl-7-(2-hydroxy-3-(4-(2-methoxyphenyl) piperazin-1-yl)propoxy)-4-methyl-2H-chromen-2-one (compound 11) exhibited a significant 5-HT1A antagonistic profile. Computational studies revealed the structural details responsible for the high affinity of selected derivatives, which were compared to known 5HT1A partial agonists.
Maternal Melatonin Contributes to Offspring Hair Follicle Development Through Transcriptional Regulation of the AP-1 Complex and MAPK Pathway
Authors: Feng Yang , Yang Ruixin , Zhang Jianqiang , Yuan Haonan , Yan Zunqiang , Wang Pengfei , Ma Xiaochun , Liu Ting , Gun Shuangbao
Published: February 2025

Maternal melatonin (MT) readily crosses the placental barrier to enter the fetal circulation, and it holds the potential to enhance hair follicle (HF) development, possibly augmented through nutritional interventions during pregnancy. However, the specific impact of maternal MT treatment on fetal HF development remains largely unexplored. In this study, we implanted pregnant rabbits with 10 mg of MT-containing and non-MT-containing silica gel microcapsules. We then assessed HF density and the extent of HF cell apoptosis in the neonatal rabbits. Our findings revealed that maternal MT implantation significantly reduced HF cell apoptosis and promoted an increased HF density in the neonates. Mechanistically, this process involved MT downregulating the expression of JUN/FOS and AP-1, while concurrently upregulating equol expression and reducing norepinephrine levels. Analysis of key protein expression within the MAPK pathway indicated that maternal MT activated this pathway. These results suggest that maternal MT treatment promotes beneficial HF development in offspring. Notably, the transcriptional regulation of JUN/FOS members of the AP-1 complex emerges as a pivotal factor mediating the beneficial effects of MT on neonatal hair follicle development.
Genomic Analysis of Hexokinase Genes in Foxtail Millet (Setaria italica): Haplotypes and Expression Patterns Under Abiotic Stresses
Authors: Zhou Wei , Cao Xiaoning , Li Hangyu , Cui Xiaokuo , Diao Xianmin , Qiao Zhijun
Published: February 2025

Hexokinases (HXKs) in plants are multifunctional enzymes that not only phosphorylate hexose but also function as glucose sensors, integrating nutrient, light, and hormone signaling networks to regulate cell metabolism and signaling pathways, thereby controlling growth and development in response to environmental changes. To date, limited information is available regarding the HXKs of foxtail millet (Setaria italica L.). In this study, six HXK genes were identified and characterized in foxtail millet. Phylogenetic analysis revealed that the foxtail millet hexokinases were classified into three subfamilies, corresponding to the two types (B-type and C-type) of hexokinases in plants. Gene structure and conserved motif analysis showed that the SiHXKs exhibited varying numbers of introns and exons, with proteins in each subfamily showing similar motif organization. Evolutionary divergence analysis indicated that the foxtail millet HXK and green foxtail HXK genes families underwent both positive and negative selection and experienced a large-scale duplication event approximately 1.18–154.84 million years ago. Expression analysis revealed that these genes are widely expressed in roots, stems, leaves, panicles, anthers, and seeds, with most genes showing significantly increased expression in roots under abiotic stress conditions, including 20% PEG 6000 (drought stress), 200 μmol/L NaCl (salt stress), and 1 μmol/L BR (brassinosteroid-mediated stress response). These results suggest that these genes may play a pivotal role in enhancing stress tolerance. Subcellular localization assay showed that SiHXK5 and SiHXK6 were predominantly localized in mitochondria. Haplotype analysis revealed that SiHXK3-H1 was associated with higher plant height and grain yield. These findings provide valuable insights into the functional characteristics of HXK genes, especially in the context of marker-assisted selection and the pyramiding of advantageous haplotypes in foxtail millet breeding programs.
A Comprehensive Review of Recent Advances in Perovskite Materials: Electrical, Dielectric, and Magnetic Properties
Authors: Tayari Faouzia , Teixeira Silvia Soreto , Graca Manuel Pedro F. , Nassar Kais Iben
Published: February 2025

Perovskite materials have emerged as one of the most promising classes of compounds in recent years due to their unique combination of electrical, dielectric, and magnetic properties, which make them ideal candidates for a wide range of advanced technological applications. This comprehensive review explores the latest developments in the electrical, dielectric, and magnetic behavior of perovskites, providing an in-depth analysis of the underlying mechanisms and their potential for improving device performance. The review covers the fundamental aspects of charge transport, polarization, and magnetic interactions in perovskite structures including the impact of crystal symmetry, ion migration, and external stimuli on their properties. Moreover, it highlights the various strategies used to tailor these properties through compositional engineering, doping, and structural modifications, resulting in enhanced efficiency, stability, and multifunctionality in applications such as photovoltaics, capacitors, dielectric resonators, and spintronic devices. Additionally, the paper addresses the challenges associated with the practical implementation of perovskite materials including stability issues under harsh environmental conditions and scalability for industrial applications. The review concludes with an outlook on future directions, emphasizing the need for further research to overcome these challenges and unlock the full potential of perovskite materials in next-generation electronics, energy storage, and magnetic devices.
Association Between Joint Pain and Cancer in 8.45 Million Korean Adults: Insights from a National Cross-Sectional Study
Authors: Kim Taewook
Published: February 2025

Background: Joint pain, a multifactorial musculoskeletal symptom, is rising globally due to an aging population. Simultaneously, cancer is increasingly considered a chronic condition with growing prevalence and improved survival rates, similar to hypertension and diabetes. Although the association between chronic diseases such as diabetes and joint pain has been well studied, the relationship between cancer and joint pain remains underexplored, especially as cancer’s chronic disease status evolves. Methods: This study analyzed data from the Korean National Health and Nutrition Examination Survey (KNHANES V) to investigate associations between cancer and joint pain in 8,451,047 individuals, representing Koreans over 50. Descriptive analyses identified demographic characteristics and disparities in joint pain prevalence by age and sex. Multivariate logistic regression analyzed seven common cancers in relation to spine, hip, and knee pain, adjusting for various factors and the Kellgren–Lawrence radiographic grade to pinpoint cancers significantly associated with each joint pain type. Results: Analysis demonstrated significant associations between certain cancers and joint pain. Back pain was linked to gastric, liver, cervical, and lung cancers; hip pain to breast and thyroid cancers; and knee pain to liver cancer. These findings underline complex relationships that suggest further investigation is needed to clarify specific cancer-related joint pain mechanisms. Conclusions: Descriptive and regression analyses highlighted essential demographic factors and significant associations between certain cancers and joint pain types. These insights enhance understanding of cancer’s chronic impact on joint pain and underscore the need for further research to refine these associations.
Intolerance of Uncertainty and Emotion Dysregulation as Predictors of Generalized Anxiety Disorder Severity in a Clinical Population
Authors: Larochelle Sébastien , Dugas Michel J. , Langlois Frédéric , Gosselin Patrick , Belleville Geneviève , Bouchard Stéphane
Published: February 2025

Background/objectives: Several factors have been shown to play a role in the development and maintenance of generalized anxiety disorder (GAD), including intolerance of uncertainty and emotion dysregulation. Although the individual contribution of both of these factors is well documented, their combined effect has yet to be studied in a clinical population with GAD. The aim of the present study was to examine the relative contribution of intolerance of uncertainty and emotion dysregulation to the prediction of worry and GAD severity in adults with GAD. Methods: The sample consisted of 108 participants diagnosed with GAD. The participants completed measures of worry, GAD severity, depressive symptoms, intolerance of uncertainty, and emotion dysregulation. Results: Multiple regression indicated that both intolerance of uncertainty and emotion dysregulation significantly contributed to both worry and GAD severity, over and above the contribution of depressive symptoms. Of note, the model explained 36% of the variance in GAD severity scores. Conclusions: The present results provide preliminary evidence of complementarity among dominant models of GAD, and point to the potential role of integrative conceptualizations and treatment strategies for GAD.
Influence of Complete Lesion Removal During Vacuum-Assisted Breast Biopsy on the Upgrade Rate of B3 Lesions Presenting as Microcalcifications
Authors: Irmici Giovanni , Depretto Catherine , Pinto Alessandra , Della Pepa Gianmarco , D’Ascoli Elisa , De Berardinis Claudia , Bonanomi Alice , Ancona Eleonora , Ballerini Daniela , Rabiolo Lidia , Schiaffino Simone , Cozzi Andrea , Scaperrotta Gianfranco
Published: February 2025

Background: B3 lesions of the breast, for which vacuum-assisted biopsy (VABB) represents the standard tissue sampling approach, have different risks of upgrade to malignancy at surgery and/or follow-up. This study aimed to investigate if complete or partial lesion removal during VABB of B3 lesions presenting as microcalcifications influences their subsequent upgrade rate. Methods: For this retrospective single-center study, we retrieved 165 lesions diagnosed as B3 at VABB that presented solely as microcalcifications categorized as Breast Imaging Reporting & Data System (BI-RADS) 4 or 5 at mammography between January 2016 and December 2020. Surgical pathology or at least 3-year follow-up were obtained to determine potential lesion upgrade to malignancy. χ2, Fisher’s, and Mantel–Haenszel tests were performed to assess if complete lesion removal influenced upgrade rates overall and among different B3 subtypes. Results: Complete lesion removal was achieved in 99/165 cases (60.0%) and did not differ among B3 subtypes (p = 0.092). The overall upgrade rate was 8.5% (95% confidence interval [CI] 5.1–13.7%, 14/165), without statistically significant differences among B3 subtypes (p = 0.562). Conversely, completely removed lesions (4.0%, 95% CI 1.6–9.9%) had a statistically significant lower upgrade rate compared to partially removed lesions (15.2%, 95% CI 8.4–25.7%, p = 0.019). According to stratified analysis according to B3 subtypes, the odds ratio of upgrade among completely and partially removed flat epithelial atypia (0.13, 95% CI 0.00–1.45) was lower (Mantel-Haenszel test p = 0.016) than those of atypical ductal hyperplasia (0.31, 95% CI 0.02–3.17) and of lobular neoplasia (0.73, 95% CI 0.01–60.62). Conclusions: The upgrade rate of B3 lesions is significantly influenced by complete lesion removal, both overall and among different B3 subtypes.
Investigation of the Discrepancy Between Optically and Gravimetrically Calculated Fiber Volume Fraction in Flax-Fiber-Reinforced Polymer
Authors: Maier Christoph , Beckmann Alexander , Wittmann Armin , Koch Klaus Peter , Fischer Georg
Published: February 2025

The fiber volume fraction significantly influences the mechanical properties of fiber-reinforced composites. However, accurate measurements can be particularly challenging in natural-fiber-reinforced polymers. This study compared indirect methods using gravimetric and volumetric measurements with a U-Net-based direct method using micro-CT images for flax-fiber-reinforced polymers made via compression molding at 2.33–13.5 bar. A notable discrepancy was observed between the direct and indirect methods, with the latter yielding a fiber volume fraction approximately 25% lower than what could be determined optically. This difference arose from the matrix being absorbed by the fibers, resulting in a mixed region between dry fiber and pure matrix, further explained using a four-phase model. Our findings indicate that the volume fraction depended on the applied pressure. Specifically, we established a linear relationship between the fiber volume fraction and the pressure up to 9.4 bar, beyond which the fiber volume fraction plateaued. Furthermore, we examined the impact of void distribution in relation to pressure. At lower pressures, voids were distributed irregularly throughout the composite, whereas at higher pressures, the overall number of voids decreased, and they tended to concentrate primarily in the center.
Transforming Burnishing Techniques: A Novel Method to Enhance Efficiency and Surface Quality Through Tool Feed Variation
Authors: Kebede Frezgi Tesfom , Felho Csaba
Published: February 2025

Slide burnishing is a non-material-removal process that improves surface finish by plastically deforming peaks into valleys using a spherical hard tool. Conventionally, multiple passes were applied with the same setup to address residual peaks, even though the first pass achieves most of the deformation. Each pass consumes equal time and energy unless parameters like feed or force are adjusted, and changing these parameters requires another cycle of passes. This repetitive approach leads to inefficiencies in time and energy, highlighting the need for different approaches to reduce redundancy while improving surface integrity. This experiment aims to change these processes by changing the feed after each pass to help the tool follow a different path than the first round. Four levels of tool feed with three levels of force and three passes were tested in nine runs that required 27 runs in the conventional method for comparison and were used to burnish the face-milled C45 steel. The new method enhanced the initial surface with up to a 66.6% decrease in Sq, greater than by the old method, and up to 69.3% in Ssk. The new method showed more steady and predictable changes of skewness than the old method during parameter changes. The old method performed better in reducing kurtosis levels when a higher number of passes (three and four) were used. These results demonstrate significant improvement over the traditional techniques, paving the way for innovative advancements and further research in the field.
An Adaptive Prediction Framework of Ship Fuel Consumption for Dynamic Maritime Energy Management
Authors: Gao Ya , Tan Yanghui , Jiang Dingyu , Sang Peisheng , Zhang Yunzhou , Zhang Jie
Published: February 2025

Accurate prediction of fuel consumption is critical for achieving efficient and low-carbon ship operations. However, the variability of the marine environment introduces significant challenges, as it leads to dynamic changes in monitoring data, complicating real-time and precise fuel consumption prediction. To address this issue, the authors proposed an incremental learning-based prediction framework to enhance adaptability to temporal dependencies in fuel consumption data. The framework dynamically adjusts a dual adaption mechanism for input features and target labels while incorporating rolling retraining to enable continuous model updates. The effectiveness of the proposed approach was validated using a real-world dataset from an LPG carrier, where it was benchmarked against conventional machine learning models, including Random Forest (RF), Linear Regression (LR), Support Vector Machine (SVM), and Multi-Layer Perceptron (MLP). Experimental results demonstrate that the proposed approach could significantly improve prediction accuracy in both offline and online scenarios. In offline mode, the proposed framework improves the R2 of various machine learning models by at least 21.97%. In online mode, the proposed method increases R2 by at least 17.97%. This work provides a new solution for real-time fuel consumption prediction in dynamic marine environments.
Experimental and Numerical Study on the Seakeeping Performance of a Wind-Powered Generation Ship Considering Boom Configurations
Authors: Trang Nguyen Thi Huyen , Mitsuyuki Taiga , Hirakawa Yoshiaki , Sadakata Katsuaki , Nakamura Yuki
Published: February 2025

This study investigates the seakeeping performance of a wind power generation ship (WPG ship). This type of vessel uses rigid sails for propulsion and submerged turbines in the form of either two or four booms to generate energy. The research includes both tank tests and simulations using Ansys AQWA, validated with the new strip method (NSM). The vessel used in this study is the container ship KCS. Overall, the power generator increases the ship’s stability and reduces roll but has almost no impact on pitch. The findings show that the 4-boom configuration offers better stability and seakeeping than the 2-boom configuration. The ship’s speed has a significant impact on the ship’s RAO, especially roll and pitch, both for the bare hull and the hull with power generation equipment. When the ship’s speed increases slightly, the roll RAO tends to decrease, but as the speed becomes higher, the RAO tends to increase. Wind conditions notably increase the roll RAO peak, reducing stability, while pitch changes are minimal. The KCS model maintains operational capability in winds up to Beaufort scale 11.
Research on the Inversion Method of Dust Retention in Grassland Plant Canopies Based on UAV-Borne Hyperspectral Data
Authors: Zhao Yibo , Lei Shaogang
Published: February 2025

Monitoring the dust retention content in grassland plants around open-pit coal mines is of significant importance for environmental pollution monitoring and the development of dust control strategies. This paper focuses on the HulunBuir grassland in the Inner Mongolia Autonomous Region, China. UAV-borne hyperspectral data and measured dust retention content in plant canopies are used as data sources. The spectral response characteristics of canopy dust retention are analyzed, and four types of optimized spectral indices are constructed, including the difference index (DI), ratio index (RI), normalized difference index (NDI), and inverse difference index (IDI). The spectral index with the highest absolute value of the correlation coefficient with the canopy dust retention is selected as the feature variable for each spectral index. In addition, machine learning methods such as the partial least squares regression (PLSR), support vector machine (SVM), and random forest (RF) methods are used to develop models for the inversion of canopy dust retention. The results show that as the dust retention content increases, the canopy reflectance in the visible wavelength initially increases and then decreases, while the reflectance in the near-infrared wavelength gradually decreases. The spectral reflectance values at different dust retention levels exhibit significant differences in the 400–420 nm, 579–698 nm, and 714–1000 nm ranges. The four types of spectral indices constructed exhibit high correlations with the canopy dust retention content, and the spectral index with the highest absolute value of the correlation coefficient is composed of near-infrared bands. The dust retention inversion model established using the RF method is more accurate than those established using the PLSR and SVM methods and yields a higher prediction accuracy. The high canopy dust retention areas are mainly distributed within 900 m of the mining area, and the dust retention gradually decreases with distance. In addition, with increasing dust retention, the fractional vegetation cover (FVC) decreases. The results of this study provide a theoretical basis and technical support for monitoring dust retention in grassland plant canopies and for dust control measures.
State’s Techniques and Local Communities’ Strategies in Land Contestations over Agro-Based Community Forests in Myanmar
Authors: Han Phyu Phyu , Paing Win Min , Ota Masahiko , Fujiwara Takahiro
Published: February 2025

Forest tenure reforms through community-based forest management programs have gained popularity in the Global South. Agricultural land use and local forest encroachment have caused the global decline of natural forests. Most community forestry (CF) studies have considered local communities as a state intervention target, underestimating their agency in local forest management. Therefore, this study aims to scrutinize land-related and counter techniques employed by the forest department and local communities in Myanmar to determine the incongruent and insufficient arrangement of de jure procedures in state CF programs. The findings reveal that although the CF program is deployed as a land control tool to regain the “reserved forest” status, realizing its institutional goals is difficult owing to local communities’ land utilization practices. Additionally, CF’s rigid institutional approach cannot manage changing, diverse, and minute local land control techniques. Meanwhile, local communities lack the indispensable, customary arrangements, leading to unequal land use, owing to which the state has to become a guarantor of common forest resources. Thus, this nature of contesting encroached forests reveals the need to critically reconsider land rights and invoke more profound steps beyond the framing of the contemporary “bundle of rights”.
Quantifying Land Subsidence Probability and Intensity Using Weighted Bayesian Modeling in Shanghai, China
Authors: Jin Chengming , Zhan Qing , Shi Yujin , Wan Chengcheng , Zhang Huan , Zhao Luna , Liu Jianli , Tian Tongfei , Liu Zilong , Wen Jiahong
Published: February 2025

Land subsidence, a slow-onset geohazard, poses a severe threat to cities worldwide. However, the lack of quantification in terms of intensity, probability, and hazard zoning complicates the assessment and understanding of the land subsidence risk. In this study, we employ a weighted Bayesian model to explicitly present the spatial distribution of land subsidence probability and map hazard zoning in Shanghai. Two scenarios based on distinct aquifers are analyzed. Our findings reveal the following: (1) The cumulative land subsidence probability density functions in Shanghai follow a skewed distribution, primarily ranging between 0 and 50 mm, with a peak probability at 25 mm for the period 2017–2021. The proportions of cumulative subsidence above 100 mm and between 50 and 100 mm are significantly lower for 2017–2021 compared to those for 2012–2016, indicating a continuous slowdown in land subsidence in Shanghai. (2) Using the cumulative subsidence from 2017–2021 as a measure of posterior probability, the probability distribution of land subsidence under the first scenario ranges from 0.02 to 0.97. The very high probability areas are mainly located in the eastern peripheral regions of Shanghai and the peripheral areas of Chongming District. Under the second scenario, the probability ranges from 0.04 to 0.98, with high probability areas concentrated in the eastern coastal area of Pudong District and regions with intensive construction activity. (3) The Fit statistics for Scenario I and Scenario II are 67% and 70%, respectively, indicating a better fit for Scenario II. (4) High-, medium-, low-, and very low-hazard zones in Shanghai account for 14.2%, 48.7%, 23.6%, and 13.5% of the city, respectively. This work develops a method based on the weighted Bayesian model for assessing and zoning land subsidence hazards, providing a basis for land subsidence risk assessment in Shanghai.
The Neuroscience Behind Writing: Handwriting vs. Typing—Who Wins the Battle?
Authors: Marano Giuseppe , Kotzalidis Georgios D. , Lisci Francesco Maria , Anesini Maria Benedetta , Rossi Sara , Barbonetti Sara , Cangini Andrea , Ronsisvalle Alice , Artuso Laura , Falsini Cecilia , Caso Romina , Mandracchia Giuseppe , Brisi Caterina , Traversi Gianandrea , Mazza Osvaldo , Pola Roberto , Sani Gabriele , Mercuri Eugenio Maria , Gaetani Eleonora , Mazza Marianna
Published: February 2025

Background: The advent of digital technology has significantly altered ways of writing. While typing has become the dominant mode of written communication, handwriting remains a fundamental human skill, and its profound impact on cognitive processes continues to be a topic of intense scientific scrutiny. Methods: This paper investigates the neural mechanisms underlying handwriting and typing, exploring the distinct cognitive and neurological benefits associated with each. By synthesizing findings from neuroimaging studies, we explore how handwriting and typing differentially activate brain regions associated with motor control, sensory perception, and higher-order cognitive functions. Results: Handwriting activates a broader network of brain regions involved in motor, sensory, and cognitive processing. Typing engages fewer neural circuits, resulting in more passive cognitive engagement. Despite the advantages of typing in terms of speed and convenience, handwriting remains an important tool for learning and memory retention, particularly in educational contexts. Conclusions: This review contributes to the ongoing debate about the role of technology in education and cognitive development. By understanding the neural differences between handwriting and typing, we can gain insights into optimal learning strategies and potential cognitive advantages, in order to optimize educational, cognitive, and psychological methodologies.
Benefits of Combined Therapies in Burn Lesions: Enzymatic Debridement and Other Modern Approaches—Our Clinical Experience
Authors: Tecuceanu Angela , Tamaş Camelia , Sava Anca , Vatavu Ruxandra , Avram Andreea Mioara , Olaru Iulia , Ciuntu Bogdan Mihnea , Abdulan Irina Mihaela , Ciuntu Roxana , Corlade Mihaela , Hreniuc Jemnoschi Irina Mihaela , Ludușanu Andreea , Bușilă Irina , Stamate Teodor , Stan Cristinel Ionel
Published: February 2025

Background: In thermal injuries, enzymatic debridement is a viable option for treating partial- and full-thickness burns, allowing for rapid removal of damaged tissue with minimal bleeding and without sacrificing healthy dermis. Enzymatic debridement using Nexobrid® combined with negative wound pressure therapy (NWPT) appears to promote healing, as enzymatic debridement helps preserve healthy tissue integrity and epithelial reserves. We explored therapeutic alternatives following enzymatic debridement to assess healing outcomes and reduce reliance on skin grafts. Methods: 24 patients with deep or partially deep thermal burns on 5–40% of total body surface area (TBSA) underwent enzymatic debridement; then, half received NWPT and the other half were treated with topicals. Results: Enzymatic debridement effectively removed necrotic tissue and facilitated healing. Only three patients required skin grafts (<10% TBSA). Enzymatic debridement combined with NWPT expedited daily healing, reduced hospitalization days, and eliminated wound secretion, as confirmed by bacteriological examination. This approach was more effective compared to enzymatic debridement followed by topical treatments. Conclusions: Nexobrid® in combination with NWPT is a promising alternative to surgical treatment, improving healing, reducing the need for skin grafts, and alleviating pain associated with dressing changes. It may be particularly useful in extensive burns, where epithelial reserves are limited.
Acute Kidney Injury Secondary to Abdominal Compartment Syndrome: Biomarkers, Pressure Variability, and Clinical Outcomes
Authors: Muğlu Harun , İnan Kahraman Eslem , Sünger Erdem , Murt Ahmet , Bilici Ahmet , Görgülü Numan
Published: February 2025

Background and Objectives: Abdominal compartment syndrome (ACS) is a severe clinical condition caused by intra-abdominal hypertension (IAH), often observed in surgical and trauma patients. However, ACS can also develop in non-surgical patients with massive ascites, leading to acute kidney injury (AKI) due to renal hypoperfusion. This study investigates the association between intra-abdominal pressure (IAP) changes, renal biomarkers, and mortality in patients with ACS-related AKI. Materials and Methods: A prospective cohort study was conducted on 24 hospitalized patients with ascites due to malignancy, cirrhosis, or heart failure. IAP was measured via the trans-vesical method on the first and seventh days of hospitalization. Serum and urinary biomarkers, including kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), and interleukin-6 (IL-6), were assessed for their correlation with IAP changes. The primary outcome was in-hospital mortality, and the secondary outcomes included AKI progression and the effect of paracentesis on IAP reduction. Results: The overall in-hospital mortality rate was 50%. Patients who survived had significantly lower IAP on the seventh day compared to those who died (14.9 ± 3.5 mmHg vs. 20.2 ± 5.6 mmHg, p = 0.01). A 25% reduction in IAP was associated with improved kidney function and increased survival (p < 0.001). Urinary KIM-1 and serum NGAL levels showed a moderate correlation with IAP (r = 0.55, p = 0.02 and r = 0.61, p = 0.018, respectively), while IL-6 levels were significantly higher in non-survivors (p = 0.03). Paracentesis was associated with improved survival outcomes (p = 0.04). Conclusions: ACS is a critical but often overlooked cause of AKI in non-surgical patients with massive ascites. Lowering IAP significantly improves renal function and reduces mortality. Urinary KIM-1 and serum NGAL may serve as useful biomarkers for monitoring IAP changes. The early identification and management of IAH through timely interventions such as paracentesis and volume control strategies could improve patient outcomes.