bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of s-palmitoylation in neurological diseases: implication for zDHHC family
Authors: Liao Dan , Huang Yutao , Liu Dan , Zhang Haofuzi , Shi Xinyu , Li Xin , Luo Peng
Published: January 2024

S-palmitoylation is a reversible posttranslational modification, and the palmitoylation reaction in human-derived cells is mediated by the zDHHC family, which is composed of S-acyltransferase enzymes that possess the DHHC (Asp-His-His-Cys) structural domain. zDHHC proteins form an autoacylation intermediate, which then attaches the fatty acid to cysteine a residue in the target protein. zDHHC proteins sublocalize in different neuronal structures and exert dif-ferential effects on neurons. In humans, many zDHHC proteins are closely related to human neu-rological disor-ders. This review focuses on a variety of neurological disorders, such as AD (Alz-heimer’s disease), HD (Huntington’s disease), SCZ (schizophrenia), XLID (X-linked intellectual disability), attention deficit hyperactivity disorder and glioma. In this paper, we will discuss and summarize the research progress regarding the role of zDHHC proteins in these neu-rological disorders.
Unusually weak irradiation effects in anisotropic iron-based superconductor RbCa2Fe4As4F2
Authors: Torsello Daniele , Piatti Erik , Fracasso Michela , Gerbaldo Roberto , Gozzelino Laura , Yi Xiaolei , Xing Xiangzhuo , Shi Zhixiang , Daghero Dario , Ghigo Gianluca
Published: January 2024

We report on the effects of 3.5 MeV proton irradiation in RbCa2Fe4As4F2, an iron-based superconductor with unusual properties in between those of the pnictides and of the cuprate high-temperature superconductors. We studied how structural disorder introduced by ion bombardment affects the critical temperature, superfluid density and gap values by combining a coplanar waveguide resonator technique, electric transport measurements and point-contact Andreev-reflection spectroscopy. We find an unusually weak dependence of the superconducting properties on the amount of disorder in this material when compared to other iron-based superconductors under comparable irradiation conditions. The nodal multigap state exhibited by pristine RbCa2Fe4As4F2 is also robust against proton irradiation, with a two-band d-d model being the one that best fits the experimental data.
Delaying early morning workouts to protect sleep in two-a-day athletes
Authors: Youngstedt Shawn D.
Published: January 2024

Two-a-day training is common for endurance athletes with training sessions typically beginning at 6 a.m. and 3 p.m. or 4 p.m. However, the early morning workouts could contribute to significant sleep loss, especially for night owls. Chronic sleep loss over a season could result in impaired performance, as well as an increased risk of physical and mental illness. It is hypothesized that shifting the early morning workout to later in the day could have beneficial effects for these athletes. A number of obstacles could make this hypothesis difficulty to test and implement. However, such a change could have dramatic benefits for some athletes.
Combined effect of cover crops and bio-fertilizer on sustainable popcorn maize production
Authors: Dragičević Vesna , Simić Milena , Dolijanović Željko , Đorđević Snežana , Stoiljković Milovan , Dimkić Ivica , Brankov Milan
Published: January 2024

Cover crops play an important role in low-input cropping systems, increasing the use of agro-ecosystem services. Due to the lack of information about the impact of cover crops and bio-fertilizers on popcorn maize (Zea mays everta Sturt.) growth and yield quality, especially the popping volume and nutritive quality, such as concentrations of protein and mineral elements, this research aimed to provide essential information. The interrelation between popcorn maize productivity and quality with important groups of soil microorganisms presents additional novelty. The results demonstrated that field pea is a beneficial cover crop, especially when combined with a bio-fertilizer, supporting the accumulation of maize biomass, chlorophyll, yield potential, and the concentrations of protein, Ca, Mg, Fe, and Zn. In addition, field pea residues promoted N-fixing bacteria, and the number of total microorganisms, especially actinomycetes and decomposing bacteria, which could promote nutrient uptake and grain quality. Residues of cover crop mixtures, common vetch + winter oats and field pea + winter oats, promoted the total number of microorganisms in the soil, and up to the end of vegetation, a greater number of decomposition and ammonification microorganisms were found, especially when the bio-fertilizer was applied, which consequently could support greater maize biomass. Popping volume, as a main trait of popcorn maize, had the highest value in the common vetch + winter oats variant, supporting again the statement that quality traits could be enhanced in sustainable production. Unlike living cover crops, mulch mainly affected soil microbial communities and promoted the development of actinomycetes and cellulolytic microorganisms during the growing season. The results of this research could contribute to the development of sustainable popcorn maize production for improved grain quality. They could also serve as a basis for isolating beneficial soil microorganisms to develop new bio-fertilizers that could improve maize production in synergy with cover crops.
Exploring the nano-wonders: unveiling the role of Nanoparticles in enhancing salinity and drought tolerance in plants
Authors: Rehman Abdul , Khan Sana , Sun Fenlei , Peng Zhen , Feng Keyun , Wang Ning , Jia Yinhua , Pan Zhaoe , He Shoupu , Wang Lidong , Qayyum Abdul , Du Xiongming , Li Hongge
Published: January 2024

Plants experience diverse abiotic stresses, encompassing low or high temperature, drought, water logging and salinity. The challenge of maintaining worldwide crop cultivation and food sustenance becomes particularly serious due to drought and salinity stress. Sustainable agriculture has significant promise with the use of nano-biotechnology. Nanoparticles (NPs) have evolved into remarkable assets to improve agricultural productivity under the robust climate alteration and increasing drought and salinity stress severity. Drought and salinity stress adversely impact plant development, and physiological and metabolic pathways, leading to disturbances in cell membranes, antioxidant activities, photosynthetic system, and nutrient uptake. NPs protect the membrane and photosynthetic apparatus, enhance photosynthetic efficiency, optimize hormone and phenolic levels, boost nutrient intake and antioxidant activities, and regulate gene expression, thereby strengthening plant’s resilience to drought and salinity stress. In this paper, we explored the classification of NPs and their biological effects, nanoparticle absorption, plant toxicity, the relationship between NPs and genetic engineering, their molecular pathways, impact of NPs in salinity and drought stress tolerance because the effects of NPs vary with size, shape, structure, and concentration. We emphasized several areas of research that need to be addressed in future investigations. This comprehensive review will be a valuable resource for upcoming researchers who wish to embrace nanotechnology as an environmentally friendly approach for enhancing drought and salinity tolerance.
Testing plant growth promoting microorganisms in the field - a proposal for standards
Authors: Neuhoff Daniel , Neumann Günter , Weinmann Markus
Published: January 2024

In the European Union and worldwide there are a burgeoning markets for plant growth promoting microorganisms (PGPM) and other biological agents as soil improvers, bio-fertilizers, plant bio-stimulants, and biological control agents or bio-pesticides. Microbial agents have a major share in this development. The use of such products is often advertised with the promise of contributing to sustainable agricultural practices by increasing crop growth and yield and offering an alternative or substitute to decrease the dependency of agriculture on hazardeous agrochemicals. In contrast to registered microbial plant protection products, PGPM that are marketed in the EU as soil improvers or plant biostimulants, are not strictly required to have proven minimum efficacy levels under field conditions. Manufacturers only have to ensure that these products do not pose unacceptable risks to human, animal or plant health, safety or the environment. Uniform guidelines comparable to the EPPO - standards (European and Mediterranean Plant Protection Organisation) to test the efficacy in field trials are not available. This paper attempts to fill the gap. It proposes guidelines for PGPM field trial design and implementation, as well as recommendations for the type and scope of data collection and evaluation. Selected research papers from literature were evaluated to analyze, whether and to what extent the requirements are already met. The majority of the papers had a clear experimental design followed by proper data evaluation. Frequent deficiencies were the low number of tested environments and crop species, insufficient site and agronomic management description and missing data on soil humidity and temperature. Using the suggested standards is assumed to increase the expressive power of tested microbial products.
Development and validation of the Chinese family environment influencing physical activity habits scale
Authors: Zhang Xulin , Yan Jingfei , Zhu Weiqiang , Fu Xiaoya
Published: January 2024

Introduction

Adolescence represents a pivotal stage in the development of healthy behaviors, where establishing positive physical activity habits can have enduring effects on an individual's overall wellbeing. The ecological model highlights the influence of environmental factors on human behavior, with the family environment playing a significant role in shaping the physical activity habits of adolescents.

Methods

The aim of this scale is to develop a reliable and effective tool, customized for the unique societal context of China, to assess how family factors influence the physical activity habits of Chinese adolescents. Participants were recruited through stratified cluster sampling from 24 secondary schools in six Chinese provinces between October and November 2021, resulting in 1,061 participants. Analysis was conducted on 1,004 valid questionnaires, divided into two samples. Sample 1, consisting of 502 students (248 males and 254 females; Mage = 15.5), underwent item analysis and exploratory factor analysis. Sample 2, comprising 502 students (267 males and 235 females; Mage = 16.5), underwent confirmatory factor analysis and internal consistency reliability analysis.

Results

Through exploratory factor analysis, we extracted three factors comprising 15 items: “Family Environment Construction” (4 items), “Family Action Support” (6 items), and “Family Health Awareness” (5 items). The Cronbach's alpha values for these factors ranged from 0.890 to 0.894. Confirmatory factor analysis confirmed a satisfactory model fit (CMIN/DF = 1.45, SRMR = 0.027, GFI = 0.991, TLI = 0.989, RMSEA = 0.03).

Discussion

The scale demonstrated strong internal consistency and test-retest reliability, confirming its effectiveness in empirical research. This study holds significant implications for enhancing the physical activity levels of adolescents, promoting their physical and mental wellbeing, enriching their developmental experiences, and contributing to their overall sense of happiness.
An examination of the psychometric properties of the Patient Health Questionnaire-9 (PHQ-9) in a Multiracial/ethnic population in the United States
Authors: Shaff Jaimie , Kahn Geoffrey , Wilcox Holly C.
Published: January 2024

Depression and suicide are significant public health issues. The Patient Health Questionnaire-9 (PHQ-9) is commonly used to assess for symptoms of depression, but its psychometric properties within Multiracial/ethnic populations remains uncertain. In a study involving 1,012 English-speaking Multiracial/ethnic participants from the United States (US), the PHQ-9 showed strong internal consistency (α = 0.93) and supported a one-factor structure. No measurement variance was observed between Non-White and White/Non-White Multiracial/ethic subgroups. PHQ-2, with a cutoff of ≥3, identified fewer depression cases than PHQ-9 (32% vs. 40%), with sensitivities of 75–99% and specificities of 74–96%; a cutoff of ≥2 missed fewer cases. Item performance of the ninth PHQ-9 question, addressing thoughts of death or self-harm, varied across generations with younger generations more likely to endorse thoughts of death or self-harm at any level of symptom severity. The findings suggest the PHQ-9 demonstrated adequate reliability within a population of Multiracial/ethnic adults in the US; however, the use of the 9th item of the PHQ-9 may not be adequate for identifying individuals at risk for suicidal thoughts and/or behaviors, particularly for older Multiracial/ethnic adults. The lower sensitivity of the PHQ-2 with a ≥ 3 cutoff suggests a cutoff of ≥2 may be preferable to miss fewer cases of depression.
A study on the impact of mental health problems on the academic buoyancy of medical students and the mechanisms
Authors: Hu Bin , Wang Yue , Zhou Hai Tao , Li Min , Zheng Li
Published: January 2024

Objective

To analyze the effects of different family environmental backgrounds and mental health problems on academic buoyancy and to explore the potential mechanisms of their effects, using a sample of 2085 medical students in Jiangsu province.

Methods

Using the multiple linear regression to analyze the impact of mental health problems on academic buoyancy in different family environment contexts.

Results

(1) Higher family income and parental literacy implied higher levels of academic buoyancy in children; (2) mental health problems and academic buoyancy were negatively related, and generalized anxiety and uncertainty stress negatively predicted academic buoyancy levels; (3) uncertainty stress may have an indirect effect on academic buoyancy levels through a partially mediating effect of generalized anxiety.
The association between leisure activity and mental health in the older adults in China: amazing Guangchangwu
Authors: Yu Jiajun , Chiu Ya-Ling , Guu Sy-Ming , Wang Jying-Nan
Published: January 2024

Objective

Since the mental health of older adult is an important topic in the aging society, the main purpose of this study is to understand the mental health status of the older adult in China under different conditions. More importantly, although people generally believe that leisure activities can improve mental health, the impact of these activities on older adult has not yet been fully discussed. Hence, this study further explores that what kind of leisure activity is associated with mental health of the older adult given different conditions.

Methods

We conducted a cross-sectional questionnaire survey to explore the relationships of various leisure activities on mental health among older adults under different demographics. This study used the Geriatric Depression Scale short forms scale (GDS-15) to assess the mental health of older adults. Based on a sample of 2,006 participants, both two-sample t-test and ANOVA were adopted to analyze the characteristics of mental health among specific subsamples.

Results

Our findings indicated that older adults generally have higher mental health scores if they do not have chronic diseases, live with other family members, or reside in urban. First, three leisure activities including walking, Guangchangwu, and hiking have positive associations on mental health for older adults with chronic diseases. Second, the older adults living alone engaged in Guangchangwu or hiking significantly associated with their good mental health. Finally, only Guangchangwu has a significantly positive associated with the mental health of rural older adults.

Conclusions

Based on our results, the government and healthcare planners can better allocate limited resources under different conditions to promote certain leisure activities, which are helpful to enhance the mental health of older adults. Guangchangwu is an activity that meets the characteristics of Chinese culture, so we further conclude that it is significantly associated with the good mental health of older adults in China.
Coronary atherosclerosis and periodontitis have similarities in their clinical presentation
Authors: Barbosa De Accioly Mattos Marcelo , Bernardo Peixoto Camila , Geraldo de Castro Amino José , Cortes Leandro , Tura Bernardo , Nunn Martha , Giambiagi-deMarval Marcia , Sansone Carmelo
Published: January 2024

Background

Periodontitis (PE) and coronary heart disease (CHD) possess multiple mechanisms for a putative association. This case-control study compared the periodontal status among CHD subjects to controls without CHD, while also investigating atheroma invasion by known periodontal pathogens.

Methods

161 subjects participated in this study were divided into three CHD groups: No CHD, chronic CHD, acute CHD. Additional analysis involved grouping subjects according to number of atheromas: no atheroma, 1–4 atheromas, 5–18 atheromas. Data were collected from medical records, periodontal examinations, and questionnaires that included demographic, behavioral, and oral health variables. Angiographic catheterizations were analyzed according to the number of atheroma lesions, lesion size, lesion location, and atheroma lesion stability. Lipoprotein profile, inflammatory markers and cells were analyzed. The microbiological branch added 30 individuals who had their atheroma lesion and subgingival plaque analyzed using polymerase chain reaction probes against the 16 s region, red complex and Aggregatibacter actinomycetemcomitans' DNA.

Results

Subjects with CHD had high levels of systemic inflammatory markers and low levels of high-density lipoproteins compared to subjects without CHD. Subjects without CHD and clear coronaries had a prevalence of mild CAL, while individuals with more atheroma lesions had advanced CAL and more active PE. Subjects with more advanced CAL were 4 times more likely to have CHD compared to subjects with less, which is comparable to smoking. Only 4 subjects had the screened pathogens detected in atheroma, although these subjects also have the screened pathogens in subgingival plaque. However, 80% of atheromas had bacteria.

Conclusions

CHD and PE showed similarities in progression while active PE led to more atheroma lesions that also tended to be larger in size.
Ulcus Vulvae Acutum Lipschütz—UVAL: case series at a Swiss university hospital emergency room
Authors: Guareschi Vittoria , Mathevet Patrice , Jacot-Guillarmod Martine
Published: January 2024

Background

Ulcus Vulvae Acutum Lipschütz (UVAL) is a largely unknown disease with a broad and complex differential diagnosis.

Objectives

To provide a description of the main characteristics of UVAL, determine the most appropriate diagnostic process and describe the current therapeutic approach.

Methods

We designed a retrospective, descriptive cohort study using the gynecological-ER database of our institution. Inclusion criteria: female patients aged between 10 and 20 years old with suspicion of a UVAL diagnosis at CHUV's gynecological ER. Data extraction: epidemiological characteristics, clinical presentation, laboratory tests, established diagnostics, treatment, and ulcer outcomes.

Results

15 patients were included for the analysis; average age: 15 years old; 60% of patients were virgo at the time of ulcer onset; all patients had at least one flu-like symptom concomitant with the vulvar lesion; the most-performed serology was for EBV and acute disease was present in only one patient; for diagnostic purposes two biopsies were performed with both inconclusive histopathology analysis; the main prescribed treatments were: oral NSAIDs, Paracetamol, and Lidocaine gel; 93% of cases presented signs of regression; the average follow-up time was 10 days. The diagnostic algorithm of Sadoghi et al: 10 out of 15 cases were retrospectively diagnosed with UVAL by the algorithm; half were diagnosed with UVAL, and the other half received a diagnosis of “ulcers of unknown origin” at the time of the gynecological ER visit.

Conclusions

We highly recommend the diagnostic and therapeutic algorithms developed by Sadoghi et al. as valuable tools to guide clinical reasoning and, consequently, improve acute vulvar ulcers management.
Metagenomic probing toward an atlas of the taxonomic and metabolic foundations of the global ocean genome
Authors: Laiolo Elisa , Alam Intikhab , Uludag Mahmut , Jamil Tahira , Agusti Susana , Gojobori Takashi , Acinas Silvia G. , Gasol Josep M. , Duarte Carlos M.
Published: January 2024

The global ocean genome (the pool of genes in marine organisms and the functional information they encode) is a major, untapped resource for science and society with a growing range of biotechnology applications in sectors such as biomedicine, energy, and food. Shotgun sequencing and metagenomics can now be used to catalog the diversity of ocean microbial life and to explore its functional potential, but has been limited by sample coverage, access to suitable sequencing platforms, and computational capacity. Here we provide a novel synthesis of the global ocean genome based on analysis of 2,102 sampled ocean metagenomes, with gene assembly and annotation via the KAUST Metagenome Analysis Platform (KMAP) Global Ocean Gene Catalog 1.0 containing 308.6 million gene clusters. Taxonomically, we report the distribution of marine genes across the tree of life and different ocean basins and depth zone biomes. Functionally, we map its relationship to protein families and biogeochemical processes, including the major microbial metabolic pathways that process three elements that play fundamental roles in biogeochemical cycles and are relevant to climate change. These data extend our understanding of the complex, dynamic nature of the ocean microbiome and its metabolic capabilities. Further research is of critical global importance both to unlock the potential of the ocean genome and to understand and predict the effects of human-induced changes, including pollution and climate change. Further hypothesis-driven research should target under-sampled deep sea and benthic microbial communities using enhanced metagenomic methods, to better understand marine ecosystem functioning. Investment in the necessary computational capacity is essential, as are suitable intellectual property frameworks.
Effects of a stepwise alveolar recruitment maneuver on lung volume distribution in dogs assessed by computed tomography
Authors: Sanchez Ana Flávia , Ambrósio Aline Magalhães , Pinto Ana Carolina B. C. Fonseca , Pereira Marco Aurélio Amador , Andrade Felipe Silveira Rego Monteiro , Rodrigues Renata Ramos , de Carvalho Martins Alessandro Rodrigues , Baroni Carina Outi , Ferrante Bruno , Fantoni Denise Tabacchi
Published: January 2024

Background

Pulmonary atelectasis is a commonly occurs during anesthesia. In these cases, mechanical ventilation (MV) associated with alveolar recruitment maneuvers (ARMs) and positive end-expiratory pressure (PEEP) is indicated to reverse the condition, ensure adequate gas exchange and improve oxygenation. ARMs can trigger volutrauma, barotrauma, and atelectrauma. Therefore, computed tomography (CT) is the gold-standard method for monitoring lung aeration after ARM.

Objective

To evaluate lung volume distribution after stepwise ARMs using computed tomography (CT).

Methods

Twelve dogs weighing 24.0 ± 6.0 kg, aged 3 ± 1 years, of both sexes and different breeds, underwent orchiectomy or ovariohysterectomy. The animals were anesthetized and ventilated in volume-controlled mode. ARMs were then initiated by positive end-expiratory pressure (PEEP) titration (5, 10, 15, and 20 cmH2O). CT scans, cardiovascular parameters, and ventilatory mechanics were evaluated at all time points. Data were assessed for normality using the Shapiro–Wilk test and a two-way analysis of variance, followed by a post-hoc Bonferroni test to identify differences between time points. Statistical significance was attributed to a value of p of <0.05.

Results

CT demonstrated that the ARMs increased ventilation throughout the lung, including the dependent regions, with volumes that increased and decreased proportionally with PEEP titration. When they reached PEEP 10 and 5 cmH2O descending (d), they remained significantly higher than those in PEEP 0 cmH2O (baseline). Static compliance improved about 40% at PEEP 10d and PEEP 5d compared to baseline. There was an increase in heart rate (HR) from PEEP 15 increasing (i) (74.5%) to PEEP 10d (54.8%) compared to baseline. Mean arterial blood pressure (MABP) decreased approximately 9% from PEEP 15i to PEEP 15d compared to baseline.

Conclusion

Lung attenuation and regional and global volumes assessed by CT showed that maximum pulmonary aeration distribution followed by PEEP titration occurred at PEEP 20 cmH2O, maintaining the lungs normoaerated and without hyperaeration.
Palliative Care for Cancer Patients in Asia: Challenges and Countermeasures
Authors: Wang Yu , Zhang Xinqing , Huang Yilin , Ma Xiangyu
Published: January 2024

With the increasing incidence of cancer worldwide, palliative care has become an effective intervention to relieve cancer patients’ pain and improve their quality of life, although the present development of palliative medicine and hospice care in many Asian countries remains insufficient. To this end, this review comprehensively discussed the main challenges that influence the promotion of palliative medicine, from the perspective of both healthcare professionals and cancer patients. We further proposed and summarized a series of potentially effective countermeasures and solutions, including the shared decision-making modal, multidisciplinary professional cooperation, application of modern science and technology, standardization training for medical workers, personalized palliative treatment regimens, and others, aiming to improve the clinical quality of palliative care practice for cancer patients and promote the development of palliative medicine in Asian regions.
Addressing Water Stress and Climate Variability in the Mediterranean: Study of Regulated Deficit Irrigation (RDI) and Non-Irrigation (NI) in Tempranillo and Cabernet Sauvignon (Vitis vinifera L.)
Authors: Sánchez-Ortiz Antoni , Lampreave Miriam , Mateos Maria Assumpta
Published: January 2024

Climate variability in Mediterranean viticultural areas, primarily attributed to climate change, will significantly impact water requirements, consequently leading to changes in irrigation management. The primary aim of this study was to assess the response of the Tempranillo and Cabernet Sauvignon grape varieties when subjected to deficit drip irrigation (RDI), in comparison to non-irrigation (NI), during various climatic years. The defined irrigation strategies involved water application equivalent to 35% and 80% of the (ET0 (reference crop evapotranspiration) × Kc (crop coefficient)). The ecophysiology of both grapevines was evaluated through the measurement of stomatal conductance (gs), sap flow, transpiration, leaf water potential (LWP), and CO2 assimilation (A). Additionally, essential parameters including the crop coefficient (Kc), transpiration, and intrinsic water use efficiency were calculated. The information gathered from the pressure–volume curves of Cabernet Sauvignon and Tempranillo encompassed the osmotic potential at full turgor (γπ100), osmotic potential at turgor loss or 0 turgor (γπ0), water content at turgor loss (CHR0), modulus of elasticity (ε), and water potential at turgor loss 0 (γH0). The results enable a precise estimation of the water requirements for irrigation, contributing to a deeper understanding of the physiological responses of both varieties. This comprehension aids in assessing the sustainability of these vineyards amidst unexpected changes in the global mean surface temperature.
The Role of Culinary Tourism in Local Marketplace Business—New Outlook in the Selected Developing Area
Authors: Vuksanović Nikola , Demirović Bajrami Dunja , Petrović Marko D. , Radovanović Milan M. , Malinović-Milićević Slavica , Radosavac Adriana , Obradović Valentina , Ergović Ravančić Maja
Published: January 2024

Local producers constitute a crucial segment of the local economy, playing a pivotal role in driving rural development and the progress of tourism. Their avenues for showcasing products extend beyond markets, bazaars, or food events, often being integrated into a destination’s tourism offerings. Moreover, they contribute to the culinary progress within tourism. Originating from wider rural areas or nearby villages, local producers significantly impact everyday migrations, services, and financial transactions in relationships spanning suburban–urban, village–town, and cross-border cooperation. This study aims to scrutinize the social facets of the organization and work of local producers, offering insights into contemporary market processes. It also serves to illustrate cross-border cooperation and the role of culinary tourism in local business. Through qualitative data processing, we will delve into the outcomes of cross-border projects, emphasizing ethical and sustainable values rooted in territory, landscape, local culture, authenticity, and the application of culinary elements in tourism. The results will shed light on the economic, social, and cultural ramifications on markets in border regions, influencing daily life and the economy. This study will define key aspects of rural development. These research findings can inform local governments, the economy, and communities in future strategic planning for developing this market segment. Tourism, especially in hospitality, will empower rural communities to enhance financial inflow and create local employment opportunities, such as roles for vineyard tour guides or local chefs. Simultaneously, it will bolster other sectors of the local economy, such as agriculture.
Interpreting the Interaction of Genotype with Environmental Factors in Barley Using Partial Least Squares Regression Model
Authors: Bratković Kamenko , Luković Kristina , Perišić Vladimir , Savić Jasna , Maksimović Jelena , Adžić Slađan , Rakonjac Aleksandra , Matković Stojšin Mirela
Published: January 2024

Genotype by environment interaction (GEI) is a complex problem that complicates the barley selection and breeding process. The knowledge of the relationship between cereal phenology and climatic data is important for understanding GEI and the physiological pathways responsible for the interaction effect. The grain yield of twenty winter barley genotypes in six environments was observed. Factors influencing the variability were analyzed using a linear mixed model. The partial least squares regression (PLSR) model was applied to determine the most relevant environmental variables in certain stages of development that explained GEI effects. Biplot with environmental variables explained 43.7% of the GEI. The barley was generally the most sensitive to the environmental conditions (relative humidity, maximum temperature and its variation, sun hours, and precipitation) during the anthesis and filling stage (May) which caused GEI. Temperature variables did not show significance only in the vegetative phase. Different genotypes responded differently to environmental factors. Genotypes NS-525, NS-589, and J-103 were highlighted as widely adaptable, and Zaječar was a suitable and reliable location for yield testing. The GEI information presented in this paper can be useful in traditional plant breeding and future breeding programs through molecular research of crop developmental genes and examination of physiological processes in two-row barley.
Exploring Genetic Diversity in Black Gram (Vigna mungo (L.) Hepper) for Pre-Harvest Sprouting Tolerance
Authors: Verma Jyotsna , Gore Padmavati G. , Kumari Jyoti , Wankhede Dhammaprakash P. , Jacob Sherry R. , Thirumani Venkatesh Arun Kumar , Nair Ramakrishnan M. , Tripathi Kuldeep
Published: January 2024

Pre-harvest sprouting (PHS) is a condition triggered by environmental factors, particularly prevalent in humid conditions, leading to substantial yield losses in black gram. While the potential for genotypic PHS tolerance exists, it has not been thoroughly assessed in black gram. Hence, the present study aimed to delve into the genetic variation for PHS tolerance in diverse black gram (Vigna mungo (L.) Hepper) germplasm, and also to comprehend the impact of various physical and physiological traits on PHS. A diverse set of 112 black gram accessions collected across the phytogeographical zones of India were examined for their seed and pod characteristics. Water absorption by pods and seeds and fresh-seed germination was calculated by following the standard procedure given by the International Seed Testing Association. The alpha-amylase activity was measured on dry seeds (0 h), 24 h, 48 h, and 72 h after germination of each accession, using a UV-VIS spectrophotometer, and hard-seededness was measured using a texture analyzer machine. The results showed a wide range in PHS tolerance and FSG, and 13 accessions were found to be PHS-tolerant (PHS value < 10%). An indicator of PHS, seed germination in a pod, ranged from 2.75% in IC485641 (highly tolerant to PHS) to 95.85% in IC530501 (highly susceptible to PHS). Correlation and multivariate analysis revealed that PHS was positively correlated with water imbibition by pod and seed, fresh-seed germination and alpha-amylase activity. PHS-tolerant accessions showed a slow increase in alpha-amylase activity, in contrast to PHS-susceptible accessions. The utilization of alpha-amylase activity as a biochemical marker has the potential for evaluating PHS tolerance across various black gram accessions. The identified PHS-tolerant accessions can be used as donors in crop improvement programs aimed at developing PHS-tolerant black gram varieties.
Caregiver Burden in Small Animal Clinics: A Comparative Analysis of Dermatological and Oncological Cases
Authors: Silva Pollyana T. R. F. , Coura Fernanda M. , Costa-Val Adriane P.
Published: January 2024

Caregiver burden, a response to the challenges faced by those caring for sick loved ones, combines objective caregiving aspects with subjective experiences. This study aimed to describe the caregiver burden in guardians of ailing animals with dermatological and oncological pathologies. Additionally, this study aimed to correlate the degree of caregiver burden with the duration of the animal’s treatment, disease stability, family income, owner’s age, and the number of people living in the same household responsible for the animal’s treatment. Using a cross-sectional approach, questionnaires were administered to guardians at a veterinary hospital. Out of 182 valid responses, 50.55% related to oncological patients and 49.45% to dermatological patients. Notably, 36.9% of oncological and 37.8% of dermatological guardians exhibited a clinically significant burden, with no statistical differences between the groups. Of the respondents, 84.6% were dog guardians, with 34.4% showing a significant burden. Feline guardians (15.4% of respondents) exhibited a higher burden (53.5%) compared to dog guardians (p = 0.02). Disease stability, time since diagnosis, and guardian age significantly influenced the burden, while household composition, income, and treatment duration showed no substantial impact. These findings underscore the psychosocial impact of caring for animals, emphasizing the need for a comprehensive understanding of the caregiver’s perspective.
Evaluation of Truck Cab Decontamination Procedures following Inoculation with Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus
Authors: Houston Grace E. , Jones Cassandra K. , Evans Caitlin , Otott Haley K. , Stark Charles R. , Bai Jianfa , Poulsen Porter Elizabeth G. , de Almeida Marcelo N. , Zhang Jianqiang , Gauger Phillip C. , Blomme Allison K. , Woodworth Jason C. , Paulk Chad B. , Gebhardt Jordan T.
Published: January 2024

This experiment aimed to evaluate commercially available disinfectants and their application methods against porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) on truck cab surfaces. Plastic, fabric, and rubber surfaces inoculated with PEDV or PRRSV were placed in a full-scale truck cab and then treated with one of eight randomly assigned disinfectant treatments. After application, surfaces were environmentally sampled with cotton gauze and tested for PEDV and PRRSV using qPCR duplex analysis. There was a disinfectant × surface interaction (p < 0.0001), indicating a detectable amount of PEDV or PRRSV RNA was impacted by disinfectant treatment and surface material. For rubber surfaces, 10% bleach application had lower detectable amounts of RNA compared to all other treatments (p < 0.05) except Intervention via misting fumigation, which was intermediate. In both fabric and plastic surfaces, there was no evidence (p > 0.05) of a difference in detectable RNA between disinfectant treatments. For disinfectant treatments, fabric surfaces with no chemical treatment had less detectable viral RNA compared to the corresponding plastic and rubber (p < 0.05). Intervention applied via pump sprayer to fabric surfaces had less detectable viral RNA than plastic (p < 0.05). Furthermore, 10% bleach applied via pump sprayer to fabric and rubber surfaces had less detectable viral RNA than plastic (p < 0.05). Also, a 10 h downtime, with no chemical application or gaseous fumigation for 10 h, applied to fabric surfaces had less detectable viral RNA than other surfaces (p < 0.05). Sixteen treatments were evaluated via swine bioassay, but all samples failed to produce infectivity. In summary, commercially available disinfectants successfully reduced detectable viral RNA on surfaces but did not eliminate viral genetic material, highlighting the importance of bioexclusion of pathogens of interest.
Using a Stakeholder Analysis to Implement the Belgian One Health National Report for Antimicrobial Use and Resistance
Authors: Cargnel Mickaël , Kelly Moira , Imberechts Hein , Catry Boudewijn , Filippitzi Maria-Eleni
Published: January 2024

(1) Background. Antimicrobial resistance (AMR) poses a substantial global health threat with profound economic implications. Acknowledging the imperative for a One Health (OH) strategy to combat this menace, Belgium introduced an annual national OH report, known as the “BELMAP report,” encompassing antimicrobial use (AMU) and AMR, with the first edition completed in 2021. The integration of innovations for the healthcare system demands a meticulously planned process. (2) Methods. We introduced a three-step stakeholder analysis (SA) as a prospective framework for navigating this new report process, fostering complementary collaboration, pinpointing obstacles, suggesting approaches to overcome them, and facilitating national policy development. The SA unfolds in three steps: stakeholders identify and list their relevant activities, assess their positions regarding the BELMAP report, and complete “actor mapping” of national AMR and AMU stakeholders. (3) Results. Stakeholder identification reveals a fragmented landscape of AMR and AMU activities across Belgium. Assessment of stakeholder positions uncovers diverse expectations, collaborative challenges, and resource considerations. “Actor mapping” identifies key stakeholders, emphasizing the importance of high-interest and high-power actors. (4) Conclusions. This SA approach not only provides insights into the present stakeholder landscape in Belgium, it can also serve as a blueprint for other countries in the process of developing OH reports.
Fighting Microbial Infections from Escherichia coli O157:H7: The Combined Use of Three Essential Oils of the Cymbopogon Genus and a Derivative of Esculentin-1a Peptide
Authors: Scotti Raffaella , Casciaro Bruno , Stringaro Annarita , Maggi Filippo , Colone Marisa , Gabbianelli Roberta
Published: January 2024

The absence of effective therapy against Escherichia coli O157:H7 infections has led to the need to develop new antimicrobial agents. As the use of synergistic combinations of natural antimicrobial compounds is growing as a new weapon in the fight against multidrug-resistant bacteria, here, we have tested new synergistic combinations of natural agents. Notably, we investigated a possible synergistic effect of combinations of essential oils and natural peptides to counteract the formation of biofilm. We chose three essential oils (i.e., Cymbopogon citratus, C. flexuosus and C. martinii) and one peptide already studied in our previous works. We determined the fractional inhibitory concentration (FIC) by analyzing the combination of the peptide derived from esculentin-1a, Esc(1–21), with the three essential oils. We also studied the effects of combinations by time–kill curves, scanning electron microscopy on biofilm and Sytox Green on cell membrane permeability. Finally, we analyzed the expression of different genes implicated in motility, biofilm formation and stress responses. The results showed a different pattern of gene expression in bacteria treated with the mixtures compared to those treated with the peptide or the single C. citratus essential oil. In conclusion, we demonstrated that the three essential oils used in combination with the peptide showed synergy against the E. coli O157:H7, proving attractive as an alternative strategy against E. coli pathogen infections.
Combinatorial Test Case Generation Based on ROBDD and Improved Particle Swarm Optimization Algorithm
Authors: Li Shunxin , Song Yinglei , Zhang Yaying
Published: January 2024

In applications of software testing, the cause–effect graph method is an approach often used to design test cases by analyzing various combinations of inputs with a graphical approach. However, not all inputs have equal impacts on the results, and approaches based on exhaustive testing are generally time-consuming and laborious. As a statute-based software-testing method, combinatorial testing aims to select a small but effective number of test cases from the large space of all possible combinations of the input values for the software to be tested, and to generate a set of test cases with a high degree of coverage and high error detection capability. In this paper, the reduced ordered binary decision diagram is utilized to simplify the cause–effect graph so as to reduce the numbers of both the inputs and test cases, thereby saving the testing cost. In addition, an improved particle swarm optimization algorithm is proposed to significantly reduce the computation time needed to generate test cases. Experiments on several systems show that the proposed method can generate excellent results for test case generation.
Renewable-Aware Frequency Scaling Approach for Energy-Efficient Deep Learning Clusters
Authors: Park Hyuk-Gyu , Kang Dong-Ki
Published: January 2024

Recently, renewable energy has emerged as an attractive means to reduce energy consumption costs for deep learning (DL) job processing in modern GPU-based clusters. In this paper, we propose a novel Renewable-Aware Frequency Scaling (RA-FS) approach for energy-efficient DL clusters. We have developed a real-time GPU core and memory frequency scaling method that finely tunes the training performance of DL jobs while maximizing renewable energy utilization. We introduce quantitative metrics: Deep Learning Job Requirement (DJR) and Deep Learning Job Completion per Slot (DJCS) to accurately evaluate the service quality of DL job processing. Additionally, we present a log-transformation technique to convert our non-convex optimization problem into a solvable one, ensuring the rigorous optimality of the derived solution. Through experiments involving deep neural network (DNN) model training jobs such as SqueezeNet, PreActResNet, and SEResNet on NVIDIA GPU devices like RTX3060, RTX3090, and RTX4090, we validate the superiority of our RA-FS approach. The experimental results show that our approach significantly improves performance requirement satisfaction by about 71% and renewable energy utilization by about 31% on average, compared to recent competitors.
The Drawback of Optimizing Air Cleaner Filters for the Adsorption of Formaldehyde
Authors: Schumacher Stefan , Caspari Anna , Schneiderwind Ute , Staack Katharina , Sager Uta , Asbach Christof
Published: January 2024

Air cleaners with activated carbon (AC) filters for the adsorption of gaseous pollutants are often used to improve indoor air quality. As formaldehyde is a common and health-relevant indoor air pollutant, many testing standards for air cleaners, such as GB/T 18801:2015, require the cleaning efficacy to be tested with this substance. This often persuades manufacturers to optimize the employed filters specifically for formaldehyde. However, in regions where indoor formaldehyde levels are far below the guideline values, other gaseous pollutants might be more relevant. Thus, the question arises of whether the optimization for formaldehyde can have a negative impact on the adsorption of other gases. To address this question, the clean air delivery rate (CADR) of an air cleaner was determined for different test gases with either a standard AC filter or an AC filter modified for improved formaldehyde adsorption. Although the modified AC filter performed substantially better for formaldehyde, a strong reduction in the CADR was observed for toluene and nitrogen dioxide. This is a drawback for situations in which these gases are more problematic than formaldehyde. The findings suggest using either specialized filters for different applications or blends of different adsorbants to find the best compromise for the most relevant pollutants.
The Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Antibiotic and Anticancer Agent Marinomycin
Authors: Abraham Emily , Lawther Hannah A. , Wang Yunpeng , Zarins-Tutt Joseph S. , Rivera Gerry Sann , Wu Chengcang , Connolly Jack A. , Florence Gordon , Agbo Matthias , Gao Hong , Goss Rebecca J. M.
Published: January 2024

With the rise in antimicrobial resistance, there is an urgent need for new classes of antibiotic with which to treat infectious disease. Marinomycin, a polyene antibiotic from a marine microbe, has been shown capable of killing methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VREF), as well as having promising activity against melanoma. An attractive solution to the photoprotection of this antibiotic has been demonstrated. Here, we report the identification and analysis of the marinomycin biosynthetic gene cluster (BGC), and the biosynthetic assembly of the macrolide. The marinomycin BGC presents a challenge in heterologous expression due to its large size and high GC content, rendering the cluster prone to rearrangement. We demonstrate the transformation of Streptomyces lividans using a construct containing the cluster, and the heterologous expression of the encoded biosynthetic machinery and production of marinomycin B.