bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrochemical Toluene Hydrogenation Using Binary Platinum-Based Alloy Nanoparticle-Loaded Carbon Catalysts
Authors: Imada Toyoki , Iida Yusuke , Ueda Yousuke , Chiku Masanobu , Higuchi Eiji , Inoue Hiroshi
Published: March 2021

A couple of toluene (TL) and its hydrogenation product, methylcyclohexane (MCH), are promising high-density hydrogen carriers to store and transport large amounts of hydrogen. Electrochemical hydrogenation of TL to MCH can achieve energy savings compared with hydrogenation using molecular hydrogen generated separately, and development of highly active catalysts for electrochemical TL hydrogenation is indispensable. In this study, binary Pt3M (M = Rh, Au, Pd, Ir, Cu and Ni) alloy nanoparticle-loaded carbon catalysts were prepared by a colloidal method, and their activity for electrochemical TL hydrogenation was evaluated by linear sweep voltammetry. Each Pt3M electrode was initially activated by 100 cycles of potential sweep over a potential range of 0–1.2 or 0.8 V vs. reversible hydrogen electrode (RHE). For all activated Pt3M electrodes, the cathodic current density for electrochemical TL hydrogenation was observed above 0 V, that is the standard potential of hydrogen evolution reaction. Both specific activity, cathodic current density per electrochemical surface area, and mass activity, cathodic current density per mass of Pt3M, at 0 V for the Pt3Rh/C electrode were the highest, and about 8- and 1.2-times as high as those of the commercial Pt/C electrode, respectively, which could mainly be attributed to electronic modification of Pt by alloying with Rh. The Tafel slope for each activated Pt3M/C electrode exhibited the alloying of Pt with the second metals did not change the electrochemical TL hydrogenation mechanism.
Heme Oxgenase-1, a Cardinal Modulator of Regulated Cell Death and Inflammation
Authors: Ryter Stefan W.
Published: March 2021

Heme oxygenase catalyzes the rate-limiting step in heme degradation in order to generate biliverdin, carbon monoxide (CO), and iron. The inducible form of the enzyme, heme oxygenase-1 (HO-1), exerts a central role in cellular protection. The substrate, heme, is a potent pro-oxidant that can accelerate inflammatory injury and promote cell death. HO-1 has been implicated as a key mediator of inflammatory cell and tissue injury, as validated in preclinical models of acute lung injury and sepsis. A large body of work has also implicated HO-1 as a cytoprotective molecule against various forms of cell death, including necrosis, apoptosis and newly recognized regulated cell death (RCD) programs such as necroptosis, pyroptosis, and ferroptosis. While the antiapoptotic potential of HO-1 and its reaction product CO in apoptosis regulation has been extensively characterized, relatively fewer studies have explored the regulatory role of HO-1 in other forms of necrotic and inflammatory RCD (i.e., pyroptosis, necroptosis and ferroptosis). HO-1 may provide anti-inflammatory protection in necroptosis or pyroptosis. In contrast, in ferroptosis, HO-1 may play a pro-death role via enhancing iron release. HO-1 has also been implicated in co-regulation of autophagy, a cellular homeostatic program for catabolic recycling of proteins and organelles. While autophagy is primarily associated with cell survival, its occurrence can coincide with RCD programs. This review will summarize the roles of HO-1 and its reaction products in co-regulating RCD and autophagy programs, with its implication for both protective and detrimental tissue responses, with emphasis on how these impact HO-1 as a candidate therapeutic target in disease.
Targeting the Activin Receptor Signaling to Counteract the Multi-Systemic Complications of Cancer and Its Treatments
Authors: Hulmi Juha J. , Nissinen Tuuli A. , Penna Fabio , Bonetto Andrea
Published: March 2021

Muscle wasting, i.e., cachexia, frequently occurs in cancer and associates with poor prognosis and increased morbidity and mortality. Anticancer treatments have also been shown to contribute to sustainment or exacerbation of cachexia, thus affecting quality of life and overall survival in cancer patients. Pre-clinical studies have shown that blocking activin receptor type 2 (ACVR2) or its ligands and their downstream signaling can preserve muscle mass in rodents bearing experimental cancers, as well as in chemotherapy-treated animals. In tumor-bearing mice, the prevention of skeletal and respiratory muscle wasting was also associated with improved survival. However, the definitive proof that improved survival directly results from muscle preservation following blockade of ACVR2 signaling is still lacking, especially considering that concurrent beneficial effects in organs other than skeletal muscle have also been described in the presence of cancer or following chemotherapy treatments paired with counteraction of ACVR2 signaling. Hence, here, we aim to provide an up-to-date literature review on the multifaceted anti-cachectic effects of ACVR2 blockade in preclinical models of cancer, as well as in combination with anticancer treatments.
Tumor Necrosis Factor Alpha in Amyotrophic Lateral Sclerosis: Friend or Foe?
Authors: Guidotti Giulia , Scarlata Chiara , Brambilla Liliana , Rossi Daniela
Published: March 2021

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by a massive neuroinflammatory reaction, which plays a key role in the progression of the disease. One of the major mediators of the inflammatory response is the pleiotropic cytokine tumor necrosis factor α (TNFα), mainly released within the central nervous system (CNS) by reactive astrocytes and microglia. Increased levels of TNFα and its receptors (TNFR1 and TNFR2) have been described in plasma, serum, cerebrospinal fluid and CNS tissue from both ALS patients and transgenic animal models of disease. However, the precise role exerted by TNFα in the context of ALS is still highly controversial, since both protective and detrimental functions have been reported. These opposing actions depend on multiple factors, among which includes the type of TNFα receptor activated. In fact, TNFR2 seems to mediate a harmful role being involved in motor neuron cell death, whereas TNFR1 signaling mediates neuroprotective effects, promoting the expression and secretion of trophic factors. This suggests that a better understanding of the cytokine impact on ALS progression may enable the development of effective therapies aimed at strengthening the protective roles of TNFα and at suppressing the detrimental ones.
MiR-200c-3p Contrasts PD-L1 Induction by Combinatorial Therapies and Slows Proliferation of Epithelial Ovarian Cancer through Downregulation of β-Catenin and c-Myc
Authors: Anastasiadou Eleni , Messina Elena , Sanavia Tiziana , Mundo Lucia , Farinella Federica , Lazzi Stefano , Megiorni Francesca , Ceccarelli Simona , Pontecorvi Paola , Marampon Francesco , Di Gioia Cira Rosaria Tiziana , Perniola Giorgia , Panici Pierluigi Benedetti , Leoncini Lorenzo , Trivedi Pankaj , Lenzi Andrea , Marchese Cinzia
Published: March 2021

Conventional/targeted chemotherapies and ionizing radiation (IR) are being used both as monotherapies and in combination for the treatment of epithelial ovarian cancer (EOC). Several studies show that these therapies might favor oncogenic signaling and impede anti-tumor responses. MiR-200c is considered a master regulator of EOC-related oncogenes. In this study, we sought to investigate if chemotherapy and IR could influence the expression of miR-200c-3p and its target genes, like the immune checkpoint PD-L1 and other oncogenes in a cohort of EOC patients’ biopsies. Indeed, PD-L1 expression was induced, while miR-200c-3p was significantly reduced in these biopsies post-therapy. The effect of miR-200c-3p target genes was assessed in miR-200c transfected SKOV3 cells untreated and treated with olaparib and IR alone. Under all experimental conditions, miR-200c-3p concomitantly reduced PD-L1, c-Myc and β-catenin expression and sensitized ovarian cancer cells to olaparib and irradiation. In silico analyses further confirmed the anti-correlation between miR-200c-3p with c-Myc and β-catenin in 46 OC cell lines and showed that a higher miR-200c-3p expression associates with a less tumorigenic microenvironment. These findings provide new insights into how miR-200c-3p could be used to hold in check the adverse effects of conventional chemotherapy, targeted therapy and radiation therapy, and offer a novel therapeutic strategy for EOC.
Short-Term Amygdala Low-Frequency Stimulation Does not Influence Hippocampal Interneuron Changes Observed in the Pilocarpine Model of Epilepsy
Authors: Mihály István , Molnár Tímea , Berki Ádám-József , Bod Réka-Barbara , Orbán-Kis Károly , Gáll Zsolt , Szilágyi Tibor
Published: March 2021

Temporal lobe epilepsy (TLE) is characterized by changes in interneuron numbers in the hippocampus. Deep brain stimulation (DBS) is an emerging tool to treat TLE seizures, although its mechanisms are not fully deciphered. We aimed to depict the effect of amygdala DBS on the density of the most common interneuron types in the CA1 hippocampal subfield in the lithium-pilocarpine model of epilepsy. Status epilepticus was induced in male Wistar rats. Eight weeks later, a stimulation electrode was implanted to the left basolateral amygdala of both pilocarpine-treated (Pilo, n = 14) and age-matched control rats (n = 12). Ten Pilo and 4 control animals received for 10 days 4 daily packages of 50 s 4 Hz regular stimulation trains. At the end of the stimulation period, interneurons were identified by immunolabeling for parvalbumin (PV), neuropeptide Y (NPY), and neuronal nitric oxide synthase (nNOS). Cell density was determined in the CA1 subfield of the hippocampus using confocal microscopy. We found that PV+ cell density was preserved in pilocarpine-treated rats, while the NPY+/nNOS+ cell density decreased significantly. The amygdala DBS did not significantly change the cell density in healthy or in epileptic animals. We conclude that DBS with low frequency applied for 10 days does not influence interneuron cell density changes in the hippocampus of epileptic rats.
Role of Heme-Oxygenase-1 in Biology of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells
Authors: Jeż Mateusz , Martyniak Alicja , Andrysiak Kalina , Mucha Olga , Szade Krzysztof , Kania Alan , Chrobok Łukasz , Palus-Chramiec Katarzyna , Sanetra Anna M. , Lewandowski Marian H. , Pośpiech Ewelina , Stępniewski Jacek , Dulak Józef
Published: March 2021

Heme oxygenase-1 (HO-1, encoded by HMOX1) is a cytoprotective enzyme degrading heme into CO, Fe2+, and biliverdin. HO-1 was demonstrated to affect cardiac differentiation of murine pluripotent stem cells (PSCs), regulate the metabolism of murine adult cardiomyocytes, and influence regeneration of infarcted myocardium in mice. However, the enzyme’s effect on human cardiogenesis and human cardiomyocytes’ electromechanical properties has not been described so far. Thus, this study aimed to investigate the role of HO-1 in the differentiation of human induced pluripotent stem cells (hiPSCs) into hiPSC-derived cardiomyocytes (hiPSC-CMs). hiPSCs were generated from human fibroblasts and peripheral blood mononuclear cells using Sendai vectors and subjected to CRISPR/Cas9-mediated HMOX1 knock-out. After confirming lack of HO-1 expression on the protein level, isogenic control and HO-1-deficient hiPSCs were differentiated into hiPSC-CMs. No differences in differentiation efficiency and hiPSC-CMs metabolism were observed in both cell types. The global transcriptomic analysis revealed, on the other hand, alterations in electrophysiological pathways in hiPSC-CMs devoid of HO-1, which also demonstrated increased size. Functional consequences in changes in expression of ion channels genes were then confirmed by patch-clamp analysis. To the best of our knowledge, this is the first report demonstrating the link between HO-1 and electrophysiology in human cardiomyocytes.
Spin-Crossover 2-D Hofmann Frameworks Incorporating an Amide-Functionalized Ligand: N-(pyridin-4-yl)benzamide
Authors: Ong Xandria , Ahmed Manan , Xu Luonan , Brennan Ashley T. , Hua Carol , Zenere Katrina A. , Xie Zixi , Kepert Cameron J. , Powell Benjamin J. , Neville Suzanne M.
Published: March 2021

Two analogous 2-D Hofmann-type frameworks, which incorporate the novel ligand N-(pyridin-4-yl)benzamide (benpy) [FeII(benpy)2M(CN)4]·2H2O (M = Pd (Pd(benpy)) and Pt (Pt(benpy))) are reported. The benpy ligand was explored to facilitate spin-crossover (SCO) cooperativity via amide group hydrogen bonding. Structural analyses of the 2-D Hofmann frameworks revealed benpy-guest hydrogen bonding and benpy-benpy aromatic contacts. Both analogues exhibited single-step hysteretic spin-crossover (SCO) transitions, with the metal-cyanide linker (M = Pd or Pt) impacting the SCO spin-state transition temperature and hysteresis loop width (Pd(benpy): T½↓↑: 201, 218 K, ∆T: 17 K and Pt(benpy): T½↓↑: 206, 226 K, ∆T: 20 K). The parallel structural and SCO changes over the high-spin to low-spin transition were investigated using variable-temperature, single-crystal, and powder X-ray diffraction, Raman spectroscopy, and differential scanning calorimetry. These studies indicated that the ligand–guest interactions facilitated by the amide group acted to support the cooperative spin-state transitions displayed by these two Hofmann-type frameworks, providing further insight into cooperativity and structure–property relationships.
Immune Response Failure in Paucisymptomatic Long-Standing SARS-CoV-2 Spreaders
Authors: Corrao Salvatore , Gervasi Francesco , Di Bernardo Francesca , Argano Christiano
Published: March 2021

The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly spread throughout the world. This disease has a spectrum of different clinical pictures with different outcomes. Herein, we report all the data from three paucisymptomatic patients during a hospital stay that might represent a paradigmatic example of the method by which SARS-CoV-2 is shed. We demonstrated the lack of an adequate qualitative and quantitative immune response by multiparametric flow cytometry analysis. Our data can provide a new perspective about the method by which SARS-CoV-2 is shed and the clinical weight of viral persistence. In all three cases, the long persistence of the virus and the consistent reduction in both innate and adaptative immune cells are not associated with greater disease severity. These patients might represent at least part of the population. In particular, one patient oscillated between positive and negative swab tests several times without presenting any immune response. In all three cases, the immune response failure was not associated with a clinically significant involvement, indicating that it is not the virus’s ability to impair the immune system, as well as its presence and persistence the fundamental mechanism that might causally lead to death. Finally, this kind of immune response in paucisymptomatic patients could pose a considerable danger to public health that questions the quarantine period. It is urgent to quantify the phenomenon with a large sample study.
Role of Hydrodynamics, Li+ Addition and Transformation Kinetics on the Formation of Plate-Like {001} Calcite Crystals
Authors: Matijaković Mlinarić Nives , Kontrec Jasminka , Njegić Džakula Branka , Falini Giuseppe , Kralj Damir
Published: March 2021

Plate-like calcite crystals with expressed unstable {001} planes are interesting research model for investigations of interfacial interactions of different additive molecules, but also the crystal growth mechanisms. The aim of this study is to reproducibly prepare a significant amount of well-defined plate-like calcite crystals and to investigate the critical experimental parameters. Thus, in precipitation system c(NaHCO3) = c(CaCl2) = 0.1 mol dm−3, the influence of hydrodynamic parameters (mode of mixing of the reaction components) and a presence of lithium ions Li+ within a wide range of concentrations, 0.0 mol dm−3 < c(Li+) < 1.0 mol dm−3, have been studied. In addition, the kinetics of the solution mediated transformation of the initially formed metastable polymorph, vaterite, were followed in order to reproducibly describe the formation of stable calcite with expressed unstable morphology. The results indicate that the plate-like calcite is formed predominantly when the ultrasound irradiation is applied at c(Li+) ≥ 0.3 mol dm−3. On the other hand, when the magnetic and mechanical stirring are applied at higher Li+ concentrations, truncated rhombohedral crystals in a mixture with plate-like crystals are obtained. It was also found that the Li+ addition significantly prolonged the transformation, mainly by inhibiting the crystal growth of calcite.
Sonographic Assessment of Complex Ultrasound Morphology Adnexal Tumors in Pregnant Women with the Use of IOTA Simple Rules Risk and ADNEX Scoring Systems
Authors: Czekierdowski Artur , Stachowicz Norbert , Smoleń Agata , Kluz Tomasz , Łoziński Tomasz , Miturski Andrzej , Kraczkowski Janusz
Published: March 2021

Background: To evaluate the accuracy of subjective assessment (SA), the International Ovarian Tumor Analysis (IOTA) group Simple Rules Risk (SRR) and the Assessment of Different NEoplasias in the adneXa (ADNEX) model for the preoperative differentiation of adnexal masses in pregnant women. Methods: The study population comprised 36 pregnant women (median age: 28.5 years old, range: 20–42 years old) with a mean gestation age of 13.5 (range: 8–31) weeks at diagnosis. Tumors were prospectively classified by local sonographers as probably benign or probably malignant using SA. Final tumor histological diagnosis was used as the reference standard in all cases. Logistic regression SRR and ADNEX models were used to obtain a risk score for every case. Serum CA125 and human epidydimis protein 4 (HE4) concentrations were also retrieved and the Risk of Ovarian Malignancy Algorithm (ROMA) value was calculated. The calculated predictive values included positive and negative likelihood ratios of ultrasound and biochemical tests. Results: Final histology confirmed 27 benign and 9 malignant (including 2 borderline) masses. The highest sensitivity (89%) and specificity (70%) were found for the subjective tumor assessment. Although no malignancy was classified as benign using the SRR criteria (sensitivity = 100%), the specificity of this scoring system was only 37%. At the cut-off risk level of >20%, the ADNEX model had a sensitivity of 78% and a specificity of 70%. Serum levels of CA125, HE4 and the ROMA risk model correctly identified adnexal malignant tumors with a sensitivity of 67%, 25% and 25%, respectively. Corresponding specificities were 72%, 100% and 100%, respectively. The highest positive and negative likelihood ratios were found for SA (LR+ = 3.0 and LR− = 0.16, respectively). Overall diagnostic accuracy of all predictive methods used in this study were similar (range: 70–75%) except for SRR (53%). Conclusion: Subjective assessment remains the best predictive method in complex adnexal masses found at prenatal ultrasound in pregnant women. For less experienced sonographers, both the SRR and ADNEX scoring systems may be also used for the characterization of such tumors, while serum tumor markers CA125 and HE4, along with the ROMA algorithm appear to be less accurate.
Prenatal Sonographic Features of CHARGE Syndrome
Authors: Traisrisilp Kuntharee , Chankhunaphas Wisit , Sittiwangkul Rekwan , Phokaew Chureerat , Shotelersuk Vorasuk , Tongsong Theera
Published: March 2021

CHARGE syndrome is a rare autosomal dominant disorder, associated with coloboma (C), heart defects (H), choanal atresia (A), retardation of growth and/or central nervous system (R), genitourinary anomalies (G) and ear abnormalities (E). Prenatal diagnosis of the syndrome is very rare but may be suspected when a combination of such abnormalities is identified. We describe a prenatally suspected case of CHARGE syndrome due to unique findings of cardiac defects (DORV) in combination with minor clues, including a structurally malformed ear with persistent non-response to an acoustic stimulation (which has never been prenatally described elsewhere), renal malrotation and growth restriction. Postnatal diagnosis was made based on confirmation of the prenatal findings and additional specific findings of bilateral coloboma, choanal atresia and ear canal stenosis. Finally, molecular genetic testing by whole exome sequencing of the neonate and her parents revealed a novel de novo heterozygous frameshift c.3506_3509dup variant in the CHD7 gene, confirming the clinical diagnosis of CHARGE syndrome. In conclusion, we describe unique prenatal features of CHARGE syndrome. Educationally, this is one of the rare examples of CHARGE syndrome, comprising all of the six specific anomalies as originally described; it is also supported by the identification of a specific genetic mutation. The identified genetic variant has never been previously reported, thereby expanding the mutational spectrum of CHD7. Finally, this case can inspire prenatal sonographers to increase awareness of subtle or minor abnormalities as genetic sonomarkers.
A Comparative Experimental Study of MIMO A&F and D&F Relay Nodes Using a Software-Defined Radio Platform
Authors: Verdecia-Peña Randy , Alonso José I.
Published: March 2021

The relaying technologies in co-operative systems are considered a core element in actual and future wireless communications, assisting the network by enhancing its reliability and improving its capability through exploiting co-operativity. In this paper, a co-operative system testbed based on Software Defined Radio (SDR) through Universal Software Radio Peripherals (USRPs) and the MatlabTM software is presented. The main novelty in this development of the platform is the implementation of 4G signal features, such as Physical Downlink Shared Channel (PDSCH) and Downlink Shared Channel (DL-SCH) for transport channel coding, which is one of the main contribution of the paper. The developed Multi-Input and Multi-Output (MIMO) SDR co-operative platform is capable of developing prototypes for the Relay Nodes. More specifically, the Amplify-&-Forward (A&F)—with or without Zero Forcing (ZF) and Minimum Mean Square Error (MMSE) Pre-Equalization—and Decode-&-Forward (D&F) protocols were implemented. Both Single-Input and Single-Output (SISO) and MIMO modes are supported by our testbed. The developed A&F and D&F MIMO co-operative systems in this paper utilize Orthogonal Space-Frequency Block Codes (OSFBCs) for the transmission of data symbols from the source to the destination. Our results show that relay nodes can substantially improve the Bit Error Rate (BER) and throughput in communications between the eNodeB (eNB) and User Equipment (UE). In particular, the maximum throughput achieved by conventional MIMO A&F is 9.3Mbps at SNR=16dB, which is 4Mbps higher than throughput of MIMO Non-Co-operative. It also shows the capacity improvement when considering the pre-equalization in the A&F schemes, compared to the conventional A&F Relay Node. For example, with MIMO A&F-MMSE pattern, a value of 11.8 Mbps is achieved for SNR=16dB, which is 84.8 % of the maximum system throughput (13.95 Mbps). On the other hand, the obtained results with D&F schemes far exceed those obtained with A&F strategies, achieving the maximum performance with the 2 × 2 MIMO D&F protocol from SNR=8dB. Furthermore, this work constitutes a first stage to the implementation of a 5G New-Radio Co-operative System platform.
Forest Landscape Restoration under Global Environmental Change: Challenges and a Future Roadmap
Authors: Noulèkoun Florent , Mensah Sylvanus , Birhane Emiru , Son Yowhan , Khamzina Asia
Published: March 2021

The adverse impacts of ecosystem degradation have raised the need for forest landscape restoration (FLR) to be included in international sustainability agendas. However, the path towards successful FLR implementation faces numerous biophysical, socioeconomic and governance challenges because FLR operates within complex socioecological systems. In the present study, we review and discuss FLR challenges in the context of global environmental change. We propose a roadmap consisting of five interlinked steps to overcome these challenges: (1) advancing ecological knowledge supporting FLR, (2) adapting FLR management to environmental change through strengthening globally distributed experimental networks, (3) implementing modelling approaches, (4) improving socioeconomic and governance dimensions, and (5) developing evidence-based knowledge platforms. The roadmap offers an iterative and adaptive framework for the continuous evaluation and improvement of FLR strategies and outcomes.
Climate Variability, Land Cover Changes and Livelihoods of Communities on the Fringes of Bobiri Forest Reserve, Ghana
Authors: Baffour-Ata Frank , Antwi-Agyei Philip , Nkiaka Elias
Published: March 2021

Climate variability coupled with land use and land cover changes have resulted in significant changes in forest reserves in Ghana with major implications for rural livelihoods. Understanding the link between climate variability, land use and land cover changes and rural livelihoods is key for decision-making, especially regarding sustainable management of forest resources, monitoring of ecosystems and related livelihoods. The study determined the extent to which climate variability drives land cover changes in the Bobiri forest reserve, Ghana. Landsat images from 1986, 2003, 2010 and 2014 were used to evaluate land cover changes of the Bobiri forest reserve in Ghana. Participatory research approaches including household questionnaire surveys, focus group discussions and key informant interviews were conducted in four fringe communities of the Bobiri forest reserve. Findings showed that local people perceived changes in rainfall and temperature patterns over the past years. Historical rainfall and temperature data for the study area showed increased variability in rainfall and an increasing temperature trend, which are consistent with the perception of the study respondents. Analysis of land cover satellite images showed that there has been significant transformation of closed forest to open forest and non-forest land cover types over the 28-year period (1986–2014), with an overall kappa statistic of 0.77. Between 2003 and 2014, closed forest decreased by 15.6% but settlement/bare ground and crop land increased marginally by 1.5% and 0.9%, respectively. Focus group discussions and key informant interviews revealed that increased land cover changes in the Bobiri forest reserve could partly be attributed to erratic rainfall patterns. Other factors such as logging and population growth were reported to be factors driving land cover changes. The study concluded that the Bobiri forest reserve has witnessed significant land cover changes and recommended that alternative livelihood sources should be provided to reduce the direct dependency of fringe communities on the forest for livelihood and firewood.
Heuristic Optimization of Thinning Individual Douglas-Fir
Authors: West Todd , Sessions John , Strimbu Bogdan M.
Published: March 2021

Research Highlights: (1) Optimizing mid-rotation thinning increased modeled land expectation values by as much as 5.1–10.1% over a representative reference prescription on plots planted at 2.7 and 3.7 m square spacings. (2) Eight heuristics, five of which were newly applied to selecting individual trees for thinning, produced thinning prescriptions of near identical quality. (3) Based on heuristic sampling properties, we introduced a variant of the hero heuristic with a 5.3–20% greater computational efficiency. Background and Objectives: Thinning, which is arguably the most subjective human intervention in the life of a stand, is commonly executed with limited decision support in tree selection. This study evaluated heuristics’ ability to support tree selection in a factorial experiment that considered the thinning method, tree density, thinning age, and rotation length. Materials and Methods: The Organon growth model was used for the financial optimization of even age Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) harvest rotations consisting of a single thinning followed by clearcutting on a high-productivity site. We evaluated two versions of the hero heuristic, four Monte Carlo heuristics (simulated annealing, record-to-record travel, threshold accepting, and great deluge), a genetic algorithm, and tabu search for their efficiency in maximizing land expectation value. Results: With 50–75 years rotations and a 4% discount rate, heuristic tree selection always increased land expectation values over other thinning methods. The two hero heuristics were the most computationally efficient methods. The four Monte Carlo heuristics required 2.8–3.4 times more computation than hero. The genetic algorithm and the tabu search required 4.2–8.4 and 21–52 times, respectively, more computation than hero. Conclusions: The accuracy of the resulting thinning prescriptions was limited by the quality of stand measurement, and the accuracy of the growth and yield models was linked to the heuristics rather than to the choice of heuristic. However, heuristic performance may be sensitive to the chosen models.
Combined Functionalization of Carbon Nanotubes (CNT) Fibers with H2SO4/HNO3 and Ca(OH)2 for Addition in Cementitious Matrix
Authors: Batiston Eduardo , de Matos Paulo Ricardo , Gleize Philippe Jean Paul , Fediuk Roman , Klyuev Sergey , Vatin Nikolai , Karelina Maria
Published: March 2021

Acid treatment is commonly used to improve the dispersion of carbon nanotubes (CNT) in a cementitious matrix, but it causes undesired delay on cement hydration kinetics. This work reports a combined CNT functionalization method with H2SO4/HNO3 and Ca(OH)2 for addition in a cementitious matrix. Results showed that the Ca(OH)2 exposure neutralized the active sites generated by acid exposure, compensating the delay in hydration. As a result, CNT exposed to H2SO4/HNO3 for 9 h and further Ca(OH)2 treatment led to equivalent hydration kinetics than un-treated CNT did with improved stability.
A Generalization of Quantal Response Equilibrium via Perturbed Utility
Authors: Allen Roy , Rehbeck John
Published: March 2021

We present a tractable generalization of quantal response equilibrium via non-expected utility preferences. In particular, we introduce concave perturbed utility games in which an individual has strategy-specific utility indices that depend on the outcome of the game and an additively separable preference to randomize. The preference to randomize can be viewed as a reduced form of limited attention. Using concave perturbed utility games, we show how to enrich models based on logit best response that are common from quantal response equilibrium. First, the desire to randomize can depend on opponents’ strategies. Second, we show how to derive a nested logit best response function. Lastly, we present tractable quadratic perturbed utility games that allow complementarity.
Decoding the Reproductive System of the Olive Fruit Fly, Bactrocera oleae
Authors: Gregoriou Maria-Eleni , Reczko Martin , Kakani Evdoxia G. , Tsoumani Konstantina T. , Mathiopoulos Kostas D.
Published: March 2021

In most diploid organisms, mating is a prerequisite for reproduction and, thus, critical to the maintenance of their population and the perpetuation of the species. Besides the importance of understanding the fundamentals of reproduction, targeting the reproductive success of a pest insect is also a promising method for its control, as a possible manipulation of the reproductive system could affect its destructive activity. Here, we used an integrated approach for the elucidation of the reproductive system and mating procedures of the olive fruit fly, Bactrocera oleae. Initially, we performed a RNAseq analysis in reproductive tissues of virgin and mated insects. A comparison of the transcriptomes resulted in the identification of genes that are differentially expressed after mating. Functional annotation of the genes showed an alteration in the metabolic, catalytic, and cellular processes after mating. Moreover, a functional analysis through RNAi silencing of two differentially expressed genes, yellow-g and troponin C, resulted in a significantly reduced oviposition rate. This study provided a foundation for future investigations into the olive fruit fly’s reproductive biology to the development of new exploitable tools for its control.
Detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae in Culture Negative Cerebrospinal Fluid Samples from Meningitis Patients Using a Multiplex Polymerase Chain Reaction in Nepal
Authors: Sharma Supriya , Acharya Jyoti , Caugant Dominique A. , Banjara Megha Raj , Ghimire Prakash , Singh Anjana
Published: March 2021

The rapid identification of bacteria causing meningitis is crucial as delays in the treatment increase mortality rate. Though considered as the gold standard for the laboratory diagnosis of bacterial meningitis, culture might give false negative results in a case of patients under antibiotics prior to lumbar puncture. This study aimed to detect Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae by a multiplex polymerase chain reaction (PCR) in culture-negative cerebrospinal fluid samples collected from clinically suspected meningitis cases attending different hospitals in Kathmandu, Nepal from January 2017 to December 2019. S. pneumoniae, N. meningitidis and H. influenzae were detected in 8.59% (33/384) of the specimens by PCR and 7.55% (29/384) of the specimens by culture. Correlation between culture and PCR of the same sample was good (Spearman’s rho correlation coefficient = 0.932). However, the difference in positivity between culture and PCR was statistically not significant (p value > 0.05). In four specimens, culture could not detect any of the targeted bacteria whereas PCR could detect presence of H. influenzae. PCR increases the diagnostic yield for bacterial meningitis. PCR may be considered as an adjunctive test for establishing the cause of infection in culture negative clinically suspected meningitis cases.
Evaluation of Gonadal Alterations in a Population Environmentally Exposed to a Mixture of Endocrine Active Pesticides
Authors: Requena-Mullor Mar , Navarro-Mena Angeles , Wei Ruqiong , López-Guarnido Olga , Lozano-Paniagua David , Alarcon-Rodriguez Raquel
Published: March 2021

Although there are studies that show that some pesticides produce gonadal dysfunction and gonadal cancer in different animals, there are not many studiesregardinghumans. This study determined the prevalence and risk in humans of developing ovarian or testicular dysfunction or cancer in areas with distinct exposure to pesticides, which have endocrine disrupting properties. A population-based case-control study was carried out on humans living in ten health districts of Andalusia (Southern Spain) classified as areas of high or low environmental exposure to pesticides according to agronomic criteria. The study population included 5332 cases and 13,606 controls. Data were collected from computerized hospital records between 2000 and 2018.The risk of gonadal dysfunction or cancer was significantly higher in areas with higher use of pesticides in relation to those with lower use.
Effectiveness of Conservative Treatment According to Severity and Systemic Disease in Carpal Tunnel Syndrome: A Systematic Review
Authors: Hernández-Secorún Mar , Montaña-Cortés Raquel , Hidalgo-García César , Rodríguez-Sanz Jacobo , Corral-de-Toro Jaime , Monti-Ballano Sofia , Hamam-Alcober Sami , Tricás-Moreno José Miguel , Lucha-López María Orosia
Published: March 2021

(1) Background: Carpal tunnel syndrome (CTS) is the most common peripheral neuropathy in the upper extremity. Conservative treatment has been effective for mild and moderate idiopathic CTS. However, severe CTS and systemic conditions were an exclusion criterion from the studies. The aim of this study is to review the effectiveness of conservative treatment in patients with CTS regardless of the level of severity and the presence or not of systemic diseases in the last ten years. (2) Methods: Randomized controlled clinical trials that compared the effect of conservative treatment on the Boston questionnaire and pain were selected. PubMed, PEDro, Scopus, Cochrane, and Web of Science databases were used. PRISMA statement checklist was performed. (3) Results: 876 studies were recorded, 29 were selected. Pharmacology, Electrotherapy and Manual Therapy had benefits for CTS. Electrotherapy and manual therapy could be effective for severe CTS patients with a systemic condition in the short term, but there was a low percentage of these patients included in the studies. (4) Conclusion: Some pharmacological treatments, manual therapy and electrotherapy have shown benefits for handling CTS, although the most effective combination of techniques is unknown. It would be necessary to include patients with systemic conditions in the selection criteria for future studies.
The Association of White Blood Cells and Air Pollutants—A Population-Based Study
Authors: Hung Shih-Chiang , Cheng Hsiao-Yuan , Yang Chen-Cheng , Lin Chia-I , Ho Chi-Kung , Lee Wen-Huei , Cheng Fu-Jen , Li Chao-Jui , Chuang Hung-Yi
Published: March 2021

The links of air pollutants to health hazards have been revealed in literature and inflammation responses might play key roles in the processes of diseases. WBC count is one of the indexes of inflammation, however the l iterature reveals inconsistent opinions on the relationship between WBC counts and exposure to air pollutants. The goal of this population-based observational study was to examine the associations between multiple air pollutants and WBC counts. This study recruited community subjects from Kaohsiung city. WBC count, demographic and health hazard habit data were collected. Meanwhile, air pollutants data (SO2, NO2, CO, PM10, and O3) were also obtained. Both datasets were merged for statistical analysis. Single- and multiple-pollutants models were adopted for the analysis. A total of 10,140 adults (43.2% males; age range, 33~86 years old) were recruited. Effects of short-term ambient concentrations (within one week) of CO could increase counts of WBC, neutrophils, monocytes, and lymphocytes. However, SO2 could decrease counts of WBC, neutrophils, and monocytes. Gender, BMI, and smoking could also contribute to WBC count increases, though their effects are minor when compared to CO. Air pollutants, particularly SO2, NO2 and CO, may thus be related to alterations of WBC counts, and this would imply air pollution has an impact on human systematic inflammation.
Community Water Fluoridation: Caveats to Implement Justice in Public Oral Health
Authors: Song Youngha , Kim Junhewk
Published: March 2021

Community water fluoridation (CWF), a long-established public health intervention, has been studied for scientific evidence from both of yea and nay standpoints. To justify CWF with scientific evidence inevitably leads to ethical justification, which raises the question of whether oral health is of individual concern or social responsibility. As dental caries is a public health problem, public health ethics should be applied to the topic instead of generic clinical ethics. From both pro- and anti-fluoridationists’ perspectives, CWF is a public health policy requiring a significant level of intervention. Thus, there needs to take further considerations for justifying CWF beyond the simple aspect of utility. For further ethical considerations on CWF, three caveats were suggested: procedural justice, social contexts, and maintenance of trust. The process to justify CWF should also be justified, not simply by majority rule but participatory decision-making with transparency and pluralistic democracy. Social contexts are to be part of the process of resolving conflicting values in public health interventions. Public trust in the dental profession and the oral healthcare system should be maintained over the considerations. This article suggests accountability for reasonableness as a framework to consider infringement by CWF for public justification of its implementation.
How Do We Assess Patient Skills in a Competence-Based Program? Assessment of Patient Competences Using the Spanish Version of the Prolapse and Incontinence Knowledge Questionnaire and Real Practical Cases in Women with Pelvic Floor Disorders
Authors: Sánchez-Sánchez Beatriz , Arranz-Martín Beatriz , Navarro-Brazález Beatriz , Vergara-Pérez Fernando , Bailón-Cerezo Javier , Torres-Lacomba María
Published: March 2021

Therapeutic patient education programs must assess the competences that patients achieve. Evaluation in the pedagogical domain ensures that learning has taken place among patients. The Prolapse and Incontinence Knowledge Questionnaire (PIKQ) is a tool for assessing patient knowledge about urinary (UI) and pelvic organ prolapse (POP) conditions. The aim of this study was to translate the Prolapse and Incontinence Knowledge Questionnaire (PIKQ) into Spanish and test its measurement properties, as well as propose real practical cases as a competence assessment tool. The cross-cultural adaptation was conducted by a standardized translation/back-translation method. Measurement properties analysis was performed by assessing the validity, reliability, responsiveness, and interpretability. A total of 275 women were recruited. The discriminant validity showed statistically significant differences in the PIKQ scores between patients and expert groups. Cronbach’s alpha revealed good internal consistency. The test–retest reliability showed excellent correlation with UI and POP scales. Regarding responsiveness, the effect size, and standardized response mean demonstrated excellent values. No floor or ceiling effects were shown. In addition, three “real practical cases” evaluating skills in identifying and analyzing, decision making, and problem-solving were developed and tested. The Spanish PIKQ is a comprehensible, valid, reliable, and responsive tool for the Spanish population. Real practical cases are useful competence assessment tools that are well accepted by women with pelvic floor disorders (PFD), improving their understanding and their decision-making regarding PFD.
Old Tricks, New Opportunities: How Companies Violate the International Code of Marketing of Breast-Milk Substitutes and Undermine Maternal and Child Health during the COVID-19 Pandemic
Authors: Ching Constance , Zambrano Paul , Nguyen Tuan T. , Tharaney Manisha , Zafimanjaka Maurice Gerald , Mathisen Roger
Published: March 2021

Breastfeeding is critical to maternal and child health and survival, and the benefits persist until later in life. Inappropriate marketing of breastmilk substitutes (BMS), feeding bottles, and teats threatens the enabling environment of breastfeeding, and exacerbates child mortality, morbidity, and malnutrition, especially in the context of COVID-19. These tactics also violate the International Code of Marketing of Breast-Milk Substitutes. This study identified marketing tactics of BMS companies since the start of the COVID-19 pandemic by reviewing promotional materials and activities from 9 companies in 14 countries, and the official Code reporting data from the Philippines. Eight qualitative themes emerged that indicate companies are capitalizing on fear related to COVID-19 by using health claims and misinformation about breastfeeding. Other promotional tactics such as donations and services were used to harness the public sentiment of hope and solidarity. Past studies show that these tactics are not new, but the pandemic has provided a new entry point, helped along by the unprecedented boom in digital marketing. There was a sharp increase of reported violations in the Philippines since the pandemic: 291 during the first months of the outbreak compared with 70 in all of 2019, corroborating the thematic findings. A lack of public awareness about the harm of donations and inadequate Code implementation and enforcement have exacerbated these problems. Proposed immediate action includes using monitoring findings to inform World Health Assembly (WHA) actions, targeted enforcement, and addressing misinformation about breastfeeding in the context of COVID-19. Longer-term action includes holding social media platforms accountable, raising public awareness on the Code, and mobilizing community monitoring.
How Knowledge about Stem Cells Influences Attitudes towards Breastfeeding: Case Study of Polish Women
Authors: Witkowska-Zimny Malgorzata , Majczyna Dorota
Published: March 2021

Maternal breastfeeding is associated with multiple health benefits, both for the child and the mother. Since breastfeeding rates are declining, finding new, future-oriented strategies to strengthen and support mothers’ positive attitudes towards breastfeeding need to be developed. In this paper, we describe how information about the presence of stem cells in breast milk can influence the willingness to breastfeed in the population of Polish pregnant women. A cross-sectional study involving a group of 150 pregnant women was conducted to assess the correlation between their knowledge about stem cells and their attitude towards breastfeeding. Among the respondents, only 6% claimed that they did not know anything about stem cells, but general knowledge about stem cells in the research group was poor. The survey results indicated that city residence, university degree, maternal experience and advanced pregnancy correlated with higher general knowledge regarding stem cells. Most respondents (77.3%) had no knowledge regarding the presence of stem cells in breast milk. Approximately two-thirds of mothers with earlier negative breastfeeding experience declared that information about the presence of stem cells in breast milk could have influenced the decision to continue and extend the time of breastfeeding. Hence highlighting the presence of stem cells in breast milk can be used to encourage breastfeeding as a unique activity.
Microvascular Dysfunction in Peripheral Artery Disease: Is Heat Therapy a Viable Treatment?
Authors: Anderson Cody P. , Pekas Elizabeth J. , Park Song-Young
Published: March 2021

Peripheral artery disease (PAD) is characterized by the development of atherosclerotic plaques in the lower-body conduit arteries. PAD is commonly accompanied by microvascular disease, which may result in poor wound healing, plantar ulcer development, and subsequent limb amputation. Understanding the mechanisms underlying the development of plantar ulcers is a critical step in the development of adequate treatment options for patients with PAD. Skin is classified into two major components: glabrous and non-glabrous. These skin types have unique microcirculation characteristics, making it important to differentiate between the two when investigating mechanisms for plantar ulcer development in PAD. There is evidence for a microcirculation compensatory mechanism in PAD. This is evident by the maintenance of basal microcirculation perfusion and capillary filling pressure despite a reduced pressure differential beyond an occlusion in non-critical limb ischemia PAD. The major mechanism for this compensatory system seems to be progressive vasodilation of the arterial network below an occlusion. Recently, heat therapies have emerged as novel treatment options for attenuating the progression of PAD. Heat therapies are capable of stimulating the cardiovascular system, which may lead to beneficial adaptations that may ultimately reduce fatigue during walking in PAD. Early work in this area has shown that full-body heating is capable of generating an acute cardiovascular response, similar to exercise, which has been suggested as the most efficient treatment modality and may generate adaptations with chronic exposure. Heat therapies may emerge as a conservative treatment option capable of attenuating the progression of PAD and ultimately impeding the development of plantar ulcers.