bluuebunny/crossref_metadata_2025 huggingface dataset:
A SÍNDROME DE BURNOUT EM AMBIENTES COM METAS E RECOMPENSAS E A INFLUÊNCIA DA MOTIVAÇÃO PARA O BEM-ESTAR DO COLABORADOR
Authors:   Universidade Presbiteriana MACKENZIE, Valim Karina Ap. Nascimento
Published: June 2022

The objective of this work is to understand the phenomenon of Burnout Syndrome, which is seen, in short, as a reaction to occupational stress in a chronic stage. Studies on Burnout syndrome were initiated by Herbert J. Freudenberger, an American psychoanalyst who, in the 1970s, used the term to address physical and mental exhaustion, as well as behavioral disorders in health professionals. This intervention is justified by the fact that in society and, above all, in companies, it has become an essential concern for physical and mental health, as well as a highly competitive factor, since the employee who is satisfied and motivated in his work, in addition to performing its tasks in the best way and with positive results, it can also generate profits for the company, since the employee who spontaneously discloses the services provided and products sold in the organization attracts new customers. As partial results of our investigation, we verified that this syndrome is presented as the result of a subjective experience of a negative character, normally constituted by attitudes, cognitions, emotions and degrading behaviors, directly related to the work, the professional role and the people with whom the individual needs to relate in their organizational environment.
The Relation Between The Book Of Life With Believers Verse Unbelievers
Authors: Sulastri Elisabeth , Eveline Sjanette
Published: June 2022

The first states that the believer's relationship in the book of life is permanently recorded. Jesus emphasized / explained on the status of believers who have been registered in the Book of Life (Luke 10:10, Hebrews 12:23). The Apostle Paul also said that partners in constant and permanent ministry are listed in the Book of Life (Phil. 4:3). The names of erasures from the Book of Life never apply to believers in Christ because believers are secure in Him, kept by the power of God (1 Pet. 1:5) and are safe in the hands of the Father and the Son (John 10:28-30). Second, how do unbelievers relate to our lives? In the book of Psalm 69, is a messianic psalm that describes the suffering of Christ caused by his enemies. Who are the enemies of Christ? Those who reject Him. What David said in Psalm 69:28, about the unbelievers, David said "May they be blotted out of the Book of Life and may they not be recorded as righteous." Why? Because their actions show not only unbelief, but a hard heart condition that prevents repentance from having faith and coming to the Messiah. The methodology used in writing this article is to study the original language words, text analysis, and interpretation to get the meaning as desired by the author. By means of theological interpretation, translation and analysis of the study of native language words. The Book of Life, especially in Daniel 12:1, has the purpose of protecting those listed in the Book of Life when tribulation comes. The Book of Life implicitly benefits those listed by providing protection to escape this great tribulation. However, for those who are not recorded in us life will suffer various punishments from God. Therefore, the names recorded in the Book of Life must be understood from, namely to protect entry into the Millennium Kingdom and enjoy all the blessings there.
Meaning Of The Word “Perish” According To 2 Corinthians 2:7
Authors: Tri Zebua Robert
Published: June 2022

The research approach in this journal uses a theological approach, specifically based on 2 Corinthians 2:7. If it is specified again, regarding the biblical texts that are considered to contain elements of Perish. Emphasis of research it tends to use a biblical theology approach. The biblical approach intends to expose the selected text. Systematics Doctrine does not get a large space or portion in this study. The results of this study can be used as the basis for the material about the word Perish in learning media or further application of learning media. Besides that, it also becomes an added value for knowledge of terms in the Bible for readers.
Seduhan Bawang Putih Terhadap Penurunan Tekanan Darah Pada Pasien Hipertensi: A Literature Review
Authors: Amir Amir , Rantesigi Nirva , Agusrianto Agusrianto
Published: June 2022

Hipertensi yang tidak terkontrol dapat menjadi penyebab utama terjadinya komplikasi seperti penyakit jantung, stroke dan gagal ginjal. Pengobatan hipertensi salah satunya dengan ramuan herbal yaitu dengan menggunakan bawang putih. Bawang putih mempunyai efek antihipertensi karena terdapat kandungan zat alisin dan hydrogen sulfide, yang bermanfaat untuk vasodilatasi pembuluh darah dan menurunkan tekanan darah. Penelitian ini bertujuan untuk mengidentifikasi studi literatur yang berhubungan dengan pemberian air seduhan bawang putih terhadap penurunan tekanan darah pada pasien hipertensi. Penelusuran artikel penelitian di beberapa database menggunakan kata kunci tertentu dalam periode tahun 2015-2019. Hasil penelusuran didapatkan 17 artikel menggunakan intervensi keperawatan, hanya 15 artikel yang memenuhi kriteria inklusi. Peneliti menilai 15 artikel tersebut dengan Duffy’s Research Appraisal Checklist Approach dan didapatkan 7 artikel termasuk kategori superior paper. Hasil literature review menunjukkan bahwa sebanyak 7 artikel menunjukkan air seduhan bawang putih mampu menurunkan tekanan darah pada pasien hipertensi. Rekomendasi literatur review ini dapat menjadikan intervensi seduhan bawang putih sebagai terapi alternatif pendamping terapi medis yang mudah didapat dan efektif dalam menurunkan tekanan darah
Análise de uma maior autonomia do Banco Central na condução da política monetária do Brasil
Authors: Júnior Armando Pereira Costa , Cordeiro Luciana Maria Costa , Rezende Luiz Paulo Fontes de , Fialho Tânia Marta Maia
Published: June 2022

Resumo: 

O objetivo deste artigo é analisar os efeitos de uma maior autonomia operacional do Banco Central do Brasil na condução da política monetária após a adoção do regime de metas de inflação em1999. Além da discussão teórica sobre autonomia do Banco Central, estimou-se um modelo da regra de Taylor no período de julho de 1994 a abril de 2015. Conclui-se que o regime de metas é instrumento eficiente para dar maior autonomia ao Banco Central na condução da política monetária. Os resultados obtidos pela regra de Taylor foram favoráveis ao crescimento e à estabilidade da economia brasileira no período analisado.
Modelagem matemática na Engenharia Ambiental: motivação à aprendizagem significativa
Authors: Silva Sara Coelho da , Lobeiro Adilandri Mercio , Macowski Diogo Heron , Correa Wellington José
Published: June 2022

Neste artigo apresenta-se o relato de uma experiência com a modelagem matemática no Ensino Superior, mais especificamente no ensino de Geometria Analítica, na Engenharia Ambiental, buscando ressaltar a importância da modelagem como fator de motivação à aprendizagem significativa de matemática nos cursos de Engenharia da UTFPR, Câmpus Campo Mourão.
À mesa com Alberto Manguel: contribuições da leitura literária na formação docente
Authors: Oliveira Bruno Pires de , Cunha Renata Cristina Oliveira Barrichelo
Published: June 2022

A pesquisa teve como objetivo identificar, nos ensaios de Alberto Manguel, as funções da leitura e contribuições da leitura literária para a formação dos sujeitos e, em especial, relacionar as experiências de leitura ao desenvolvimento pessoal e profissional docente, extraindo lições para as práticas de formação inicial e continuada de professores. Com a sistematização das leituras das obras é possível afirmar que a literatura se faz indispensável nos processos formativos na medida em que promove, a partir da leitura criadora, autoconhecimento, consciência das relações com/no mundo e possibilidade de questionar e transformar a prática docente.
Repensando a infância, com Bourdieu
Authors: Alanen Leena
Published: June 2022

Leena Alanen é um dos principais nomes da Sociologia da Infância de Língua Inglesa. Seu pensamento sustenta a Sociologia da Infância Estrutural-relacional e traz para o debate conceitos e ideias desenvolvidas pelo sociólogo francês Pierre Bourdieu, para o qual as gerações não se definem apenas em termos de idade, mas dependem das relações. A proposta da autora neste texto publicado em sueco em 2009 e que agora apresentamos em português ao conjunto de pesquisadores brasileiros e portugueses é reafirmar a abordagem relacional da Sociologia da Infância e trazer para o Estudo da Infância os conceitos desenvolvidos por Bourdieu, constituindo o “campo”, por exemplo, como objeto de estudo sociológico e um conceito útil também para o desenvolvimento dos Estudos Sociais das Crianças e da Infância. Para além dos elementos teóricos oferecidos pela teoria de Bourdieu, Alanen destaca a análise dos campos, das posições ocupadas por cada um de seus integrantes, bem como do papel desempenhado por eles nas disputas inerentes a cada campo como uma metodologia possível de ser estendida ao estudo das crianças e da infância, tendo em visa a compreensão das famílias, por exemplo, como um campo intergeracional.
A dimensão pública das instituições de educação superior comunitárias
Authors: Lückmann Luiz Carlos , Cimadon Aristides
Published: June 2022

O texto analisa alguns pressupostos que se fizeram presentes no processo de regulação do novo marco legal das Instituições Comunitárias de Educação Superior (ICES), consubstanciado na Lei nº 12.881/13. Até então, as ICES vinham sendo tratadas pelo Estado brasileiro ora como instituições públicas, ora como instituições privadas, produzindo distorções de ordem conceitual e legal. Com a nova Lei, as ICES passam a ser reconhecidas como um modelo diferenciado de educação superior, cujas características são marcadas pela natureza pública de seus atos instituidores, pela regionalidade de atuação e pelo seu comprometimento com o processo de desenvolvimento social de comunidades interioranas do país.
v. 7, n. 13 (2015)
Authors: Nupem Revista
Published: June 2022

Vol. 7, No 13 (2015)
A santificação da figura feminina na literatura medieval: um estudo de caso de The Fisician's Tale de Geoffrey Chaucer
Authors: Medeiros Márcia Maria , Zimmermann Tânia Regina
Published: June 2022

Um dos mais populares tipos de literatura medieval é representado pela hagiografia, textos com caráter didático-pedagógicos utilizados pela Igreja, a fim de ensinar o cristianismo à população de forma simples, uma vez que a maioria das pessoas era analfabeta. Uma das principais características destas obras é colocada na santificação da figura feminina, através de recursos que gradualmente vão destruindo os elementos constitutivos da ideia de feminilidade (corte de cabelo, desfigurar o corpo), a fim de alcançar esta sacralização. O texto proposto para estudo neste artigo se encaixa neste contexto, e o propósito do trabalho a ser realizado será o de compreender como representações do feminino são idealizadas neste poema, a fim de compreender as estratégias concebidas para este processo de santificação.
Circulação urbana: um estudo sobre a mobilidade em Ituiutaba, MG
Authors: Silva Daniel de Araujo , Loboda Carlos Roberto
Published: June 2022

A presente pesquisa teve por objetivo principal analisar as condições da mobilidade urbana, sobretudo as vias de circulação, bem como a dinâmica do trânsito na cidade de Ituiutaba, Minas Gerais. Foram analisadas as vias de circulação da cidade, com ênfase para a dinâmica e comportamento do trânsito, se atentando para os seguintes elementos: faixas de pedestres; semáforos e circulação de veículos. Em relação às vias de circulação e dinâmica do trânsito, ressaltamos que o diagnóstico aqui realizado pode ser um importante instrumento para posterior intervenção e promoção de sinalização e controle de tráfego, principalmente nos locais onde não existem, para que se tenha assim, uma circulação segura tanto para os motoristas, quanto para os pedestres em toda a cidade, considerando a cidade enquanto um grande espaço público.
Situações-limite e o espetáculo – o hiperespetáculo pelo viés da construção das identidades
Authors: Reis Pedro Henrique Baptista
Published: June 2022

Neste artigo pretendemos aproximar e articular os conceitos de espetáculo, de Guy Debord, e de situação-limite, de Karl Jaspers, afim de transpor certas aporias com os quais o pensamento debordiano se enfrenta, confronta e transforma numa época que se desenha como tecnocrática e na qual os meios de comunicação e informações tornam-se ubíquos e os sujeitos, mediados e mediadores, se veem transformados por uma vivência na presença e através desses meios.
A história oral como disciplina, a memória coletiva e a “costura artesanal” – religião, política
Authors: Alonso Leandro Seawright
Published: June 2022

Procurei demonstrar que a história oral se desenvolveu no âmbito de uma disciplinaridade; utilizei-me de excertos da literatura machadiana para demonstrar a importância das entrevistas; apresentei o conceito de “costura artesanal”. Posteriormente, utilizei-me do conceito de memória coletiva e de história oral testemunhal para demonstrar ser possível trabalhar com trauma, política e religião.
A constituição do professor de matemática na vivência do estágio supervisionado
Authors: Nehring Cátia Maria , Pozzobon Marta Cristina Cezar , Battisti Isabel Koltermann
Published: June 2022

Este artigo é resultado de uma pesquisa desenvolvida por um grupo de professoras que atuam/ram em um curso de licenciatura em Matemática de uma instituição do interior do Rio Grande do Sul. Investiga-se a seguinte questão: Como a vivência de estágio supervisionado possibilita aos licenciandos se constituírem em professores de matemática da Educação Básica? Como material empírico, consideram-se os Relatórios de Estágio do Ensino Fundamental e do Ensino Médio dos anos de 2009 a 2011, perfazendo um total de 20 trabalhos. Para dar conta de tais análises, toma-se o conceito de compreensão proposto por Lee Shulman (1987, 1992, 2005), em que destaca que as ideias e conceitos a serem ensinados, precisam ser transformadas, de modo que os licenciandos compreendam a essência do ato de raciocinar pedagogicamente, o que se constitui no exercício da docência; que o licenciando em vivência de estágio necessita compreender o conteúdo matemático, ter intencionalidade para ensinar, realizar escolhas metodológicas, refletir sobre suas escolhas e conseguir analisá-las para constituir-se como professor de matemática.
Literatura e história: aproximações e distanciamentos
Authors: Ozelame Josiele Kaminski Corso , Oliveira Raíza Brustolin de
Published: June 2022

Essa pesquisa tem como objetivo discutir semelhanças e diferenças entre a narrativa histórica e a narrativa literária, tendo em vista que, ambas as narrativas constroem conhecimento por meio da escrita e para isso se baseiam na realidade. Essa construção (ou reconstrução) pode ser realizada com mais ou menos elementos fictícios; com uma maior ou menor aproximação com o leitor; com mais ou menos autonomia do autor. Tais aspectos caracterizam ambos os tipos de narrativa, ora aproximando-as, ora as distanciando. Estes e outros fatores serão discutidos, em vista de propor que ambas são formas de olhar para o passado.
Biodiesel from Dodonaea Plant Oil: Synthesis and Characterization—A Promising Nonedible Oil Source for Bioenergy Industry
Authors: Bukhari Syeda Alia Zehra , Ullah Kifayat , Ahmad Mushtaq , Zafar Muhammad , Ullah Anwar , Sultana Shazia , Ibrahim Mohamed Mousa , Hessien Mahmoud Mohamed , Mersal Gaber Ahmad Mahmoud , Ghoneim Sherif Salama Mohamed
Published: June 2022

In this work, Dodonaea oil was studied as a potential biodiesel source. Dodonaea (Dodonaea viscosa Jacq.) is an evergreen shrubby plant that thrives in tropical and subtropical conditions. The plant produces high-grade biodiesel in terms of both quantity and quality despite its naturally high fat content. In the transesterification followed by esterification reaction, varied ratios of oil to methanol, constant temperature (60°), reaction duration (1 h), and different catalyst concentrations (0.25–0.75% (w/w) were utilized. A maximum biodiesel yield of 90% was achieved. For fuel characteristic analysis, the prepared biodiesel was specified and compared to ASTM criteria. The chemical composition was verified using analytical techniques such as FT-IR and NMR spectroscopy. As a result of the foregoing, Dodonaea is considered a possible bioenergy source, particularly in the transport sector.
Effect of the Innovative Running Shoes With the Special Midsole Structure on the Female Runners’ Lower Limb Biomechanics
Authors: Fu Fengqin , Guo Lianming , Tang Xunfei , Wang Jiayu , Xie Zhihao , Fekete Gusztáv , Cai Yuhui , Hu Qiuli , Gu Yaodong
Published: June 2022

The study aimed to research the effects of innovative running shoes (a high heel-to-toe drop and special structure of midsole) on the biomechanics of the lower limbs and perceptual sensitivity in female runners. Fifteen healthy female runners were recruited to run through a 145-m runway with planted force plates at one peculiar speed (3.6 m/s ± 5%) with two kinds of shoe conditions (innovative running shoes vs. normal running shoes) while getting biomechanical data. The perception of shoe characteristics was assessed simultaneously through a 15-cm visual analog scale. The statistical parametric mapping technique calculated the time-series parameters. Regarding 0D parameters, the ankle dorsiflexion angle of innovative running shoes at touchdown was higher, and the peak dorsiflexion angle, range of motion, peak dorsiflexion velocity, and plantarflexion moment on the metatarsophalangeal joint of innovative running shoes during running were significantly smaller than those of normal running shoes (all p < 0.001). In addition, the braking phase and the time of peak vertical force 1 of innovative running shoes were found to be longer than those of normal running shoes (both p < 0.05). Meanwhile, the average vertical loading rate 1, peak vertical loading rate 1, peak braking force, and peak vertical force 1 in the innovative running shoes were lower than those of the normal running shoes during running (both p < 0.01). The statistical parametric mapping analysis exhibited a higher ankle dorsiflexion angle (0–4%, p < 0.05), a smaller knee internal rotation angle (0–6%, p < 0.05) (63–72%, p < 0.05), a decreased vertical ground reaction force (11–17%, p = 0.009), and braking anteroposterior ground reaction force (22–27%, p = 0.043) for innovative running shoes than normal running shoes. Runners were able to perceive the cushioning of innovative running shoes was better than that of normal running shoes. These findings suggested combining the high offset and structure of the midsole would benefit the industrial utilization of shoe producers in light of reducing the risk of running injuries for female runners.
Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar
Authors: Yan Huiling , Zhang Xinxin , Li Xiang , Wang Xuelai , Li Hanxi , Zhao Qiushuang , Yin Peng , Guo Ruixue , Pei Xiaona , Hu Xiaoqing , Han Rui , Zhao Xiyang
Published: June 2022

Populus alba × Populus glandulosa (84K poplar) is model material with excellent genetic engineering resource and ornamental value. In our study, AmRosea1 (Antirrhinum majus) was overexpressed in 84K poplar, and the transgenic 84K (AM) poplar with high content of anthocyanin exhibited red pigmentation leaves. The transcriptome analysis between wild type (WT) and AM showed that 170 differentially expressed genes (DEGs) (86 up-regulated and 84 down-regulated) were found, and some DEGs were involved in flavone and flavonol biosynthesis, flavonoid biosynthesis and anthocyanin biosynthesis. The metabolome analysis showed that 13 anthocyanins-related differentially accumulated metabolites (DAMs) were detected in AM. The correlation analysis between DEGs and DAMs were performed, and the results revealed that 18 DEGs, including 11 MYB genes, two BZ1 genes, one FG2 gene, one ANS gene, and three IF7MAT genes, were negatively or positively correlated with 13 DAMs. The phylogenetic analysis demonstrated that there was high homology between AmRosea1 and PagMYB113, and MYB113 co-expressed with BZ1, ANS and DFR directly. Our results elucidated the molecular mechanism of plant color change mediated by anthocyanin biosynthesis pathway, which laid the foundation for the development and utilization of colorful woody plant.
Titanate Nanotubes-Based Heterogeneous Catalyst for Efficient Production of Biomass Derived Chemicals
Authors: Zhou Shuolin , Wu Lu , Bai Junzhuo , Liu Xianxiang , Lei Min , Long Min , Huang Keying
Published: June 2022

The development of efficient heterogeneous catalytic system to convert plentiful biomass to renewable bio-chemicals is urgent need. Titanate nanotubes-based materials obtained from hydrothermal treatment have been reported as low-cost and efficient catalytic materials in chemical syntheses for bio-based chemicals production with interesting catalytic performance. This mini-review expressly revealed the significance and potential of using titanate nanotubes based material as sustainable and environmentally benign solid catalysts/supports for synthesis of various bio-based chemicals, including glycerol-derived solketal, jet fuel range alkanes precursors, biomass-derived esters, aldehydes, aromatic compounds and so on. From the current knowledge on titanate nanotubes-based material via hydrothermal method here summarized, the future lines of research in the field of catalysis/supports for bio-based chemicals production are outlined.
High Dose Intramuscular Vitamin D3 Supplementation Impacts the Gut Microbiota of Patients With Clostridioides Difficile Infection
Authors: Lee Sang Hoon , Park Han-Ki , Kang Chang Don , Choi Dae Hee , Park Sung Chul , Park Jin Myung , Nam Seung-Joo , Chae Gi Bong , Lee Kyoung yul , Cho Hyunseok , Lee Sung Joon
Published: June 2022

Background and Aim

Current therapeutic strategies for Clostridioides difficile infections (CDI), including oral vancomycin, metronidazole and fecal microbial transplantation, have limited efficacy and treatment failure may occur in as many as one- third of cases. Recent studies have reported that lower concentrations of 25-hydroxyvitamin D are associated with CDI severity and recurrence. However, there have been no studies on microbiota composition after the administration of vitamin D in patients with CDI. Therefore, our study aimed to compare the microbiota composition between the two groups, including eight CDI-positive patients with vitamin D supplementation and ten CDI-positive patients without vitamin D supplementation by using 16S rRNA microbial profiling.

Methods

Twenty subjects were enrolled in this prospective randomized controlled study. One subject dropped out due to lack of contact with the guardian after discharge and one subject dropped out due to withdrawal of consent. Thus, 18 patients with CDI and vitamin D insufficiency (vitamin D level < 17 ng/mL) were divided into two groups: CDI with vitamin D supplementation (n = 8) and CDI without vitamin D supplementation (control: n = 10). Subjects with vitamin D insufficiency were randomized to receive 200,000 IU intramuscular cholecalciferol whereas patients in the control group received only oral vancomycin. Stool samples were obtained twice before vancomycin was administered and eight weeks after treatment; the V3-V4 16S rRNA metagenomic sequencing was performed using EzBioCloud.

Results

The alpha diversity of the gut microbiota in the recovery state was significantly higher than that in the CDI state. Analysis of bacterial relative abundance showed significantly lower Proteobacteria and higher Lachnospiraceae, Ruminococcaceae, Akkermansiaceae, and Bifidobacteriaceae in the recovery state. When comparing the control and vitamin D treatment groups after eight weeks, increase in alpha diversity and, abundance of Lachnospiraceae, and Ruminococcaceae exhibited the same trend in both groups. A significant increase in Bifidobacteriaceae and Christensenellaceae was observed in the vitamin D group; Proteobacteria abundance was significantly lower in the vitamin D treatment group after eight weeks than that in the control group.

Conclusion

Our study confirmed that the increase in the abundance of beneficial bacteria such as Bifidobacteriaceae, and Christensenellaceae were prominently evident during recovery after administration of a high dose of cholecalciferol. These findings indicate that vitamin D administration may be useful in patients with CDI, and further studies with larger sample sizes are required.
Angiographic Lesion Morphology Provides Incremental Value to Generalize Quantitative Flow Ratio for Predicting Myocardial Ischemia
Authors: Zhang Jie , Zhao Na , Xu Bo , Xie Lihua , Yin Weihua , An Yunqiang , Yan Hankun , Yu Yitong , Lu Bin
Published: June 2022

Aim

The quantitative flow ratio (QFR) is favorable for functional assessment of coronary artery stenosis without pressure wires and induction of hyperemia. The aim of this study was to explore whether angiographic lesion morphology provides incremental value to generalize QFR for predicting myocardial ischemia in unselected patients.

Methods

This study was a substudy to the CT-FFR CHINA trial, referring 345 participants from five centers with suspected coronary artery disease on coronary CT angiography for diagnostic invasive coronary angiography (ICA). Fractional flow reserve (FFR) was measured in all vessels with 30–90% diameter stenosis. QFR was calculated in 186 lesions from 159 participants in a blinded manner. In addition, parameters to characterize lesion features were recorded or measured, including left anterior descending arteries (LADs)-involved lesions, side branch located at stenotic lesion (BL), multiple lesions (ML), minimal lumen diameter (MLD), reference lumen diameter (RLD), percent diameter stenosis (%DS), lesion length (LL), and LL/MLD4. Logistic regression was used to construct two kinds of models by combining single or two lesion parameters with the QFR. The performances of these models were compared with that of QFR on a per-vessel level.

Results

A total of 148 participants (mean age: 59.5 years; 101 men) with 175 coronary arteries were included for final analysis. In total, 81 (46%) vessels were considered hemodynamically significant. QFR correctly classified 82.29% of the vessels using FFR with a cutoff of 0.80 as reference standard. The area under the receiver operating characteristic curve (AUC) of QFR was 0.86 with a sensitivity, specificity, positive predictive value, and negative predictive value of 80.25, 84.04, 81.25, and 83.16%, respectively. The combined models (QFR + LAD + MLD, QFR + LAD + %DS, QFR + BL + MLD, and QFR + BL + %DS) outperformed QFR with higher AUCs (0.91 vs. 0.86, P = 0.02; 0.91 vs. 0.86, P = 0.02; 0.91 vs. 0.86, P = 0.02; 0.90 vs. 0.86, P = 0.03, respectively). Compared with QFR, the sensitivity of the combined models (QFR + BL and QFR + MLD) was improved (91.36 vs. 80.25%, 91.36 vs. 80.25%, respectively, both P < 0.05) without compromised specificity or accuracy.

Conclusion

Combined with angiographic lesion parameters, QFR can be optimized for predicting myocardial ischemia in unselected patients.
Photoperiod and Temperature as Dominant Environmental Drivers Triggering Plant Phenological Development of American Ginseng Along With Its Quality Formation
Authors: Chen Tingting , Wang Liqin , Wang Hongtao , Jiang Sheng , Zhou San
Published: June 2022

American ginseng (Panax quinquefolius L.) has been cultivated in many locations in China, and we have noticed that its phenological performance and quality characteristics varied from location to location. However, the exogenous factors driving this process are still poorly understood, and this reduces the successful introduction of American ginseng for quality ensuring. Here, we conducted field and cabinet experiments to explore the relationship among environmental factors, phenological development, and ginsenoside accumulation in American ginseng. In the field experiments, we found that American ginseng from different original locations showed different phenological rhythms and different ginsenoside accumulation. Especially, those from higher latitude locations (e.g., Wuchang, 44.55°N) presented delayed spring phenology and advanced autumn phenology, but higher ginsenoside contents along with higher gene expression levels of ginsenoside biosynthesis than those from lower latitude locations (e.g., Wendeng, 37.23°N). Data analysis indicated that the phenological rhythm of American ginseng is closely related to the seasonal change in environmental factors, especially winter chilling duration, spring warming, and autumn day-length shortening. In the cabinet experiments, results further proved that temperature interaction with photoperiod plays a decisive role in the phenological development and ginsenoside accumulation of American ginseng in the absence of water and nutrient limitation. Both field and cabinet experiments indicated that sufficient winter cold exposure coupled with spring long day-length photoperiod is a beneficial environmental factor for normal spring phenology development, while shortening autumn day-length coupled with autumn cooling is a beneficial environmental factor for normal autumn phenology development of American ginseng. Results also indicated that insufficient cold exposure and unsuitable day-length photoperiod are detrimental factors to both spring and autumn phenology, and are not good for ginsenoside accumulation in American ginseng. An understanding of the environmental factors influencing phenological development along with ginsenoside accumulation can provide guidance for predicting suitable cultivation locations and improving planting techniques for high yield and good quality of American ginseng.
Riverbed Temperature and 4D ERT Monitoring Reveals Heterogenous Horizontal and Vertical Groundwater-Surface Water Exchange Flows Under Dynamic Stage Conditions
Authors: Johnson Tim , Thomle Jon , Stickland Chris , Goldman Amy , Stegen James
Published: June 2022

Groundwater surface water exchange plays a critical role in physical, biological, and geochemical function of coastal and riverine systems. Observing exchange flow behavior in heterogeneous systems is a primary challenge, particularly when flows are governed by dynamic river stage or tidal variations. In this paper we demonstrate a novel application of time-lapse 3D electrical resistivity tomography and temperature monitoring where an array of thermistors installed beneath a riverbed double as resistivity electrodes. We use the array to monitor stage driven exchange flows over a 6-day period in a dynamic, stage-driven high order stream. We present a method for addressing the otherwise confounding effects of the moving river-surface boundary on the raw resistivity data, thereby enabling successful tomographic imaging. Temperature time-series at each thermistor location and time-lapse 3D images of changes in bulk electrical conductivity together provide a detailed description of exchange dynamics over a 10-meter by 45-meter section of the riverbed, to a depth of approximately 5 m. Results reveal highly variable flux behavior throughout the monitoring domain including both horizontal and vertical exchange flows.
The rs10830963 Polymorphism of the MTNR1B Gene: Association With Abnormal Glucose, Insulin and C-peptide Kinetics
Authors: Vejrazkova Daniela , Vankova Marketa , Vcelak Josef , Krejci Hana , Anderlova Katerina , Tura Andrea , Pacini Giovanni , Sumova Alena , Sladek Martin , Bendlova Bela
Published: June 2022

Background

The MTNR1B gene encodes a receptor for melatonin, a hormone regulating biorhythms. Disruptions in biorhythms contribute to the development of type 2 diabetes mellitus (T2DM). Genetic studies suggest that variability in the MTNR1B gene affects T2DM development. Our aim was to compare the distribution of the genetic variant rs10830963 between persons differing in glucose tolerance in a sample of the Czech population (N=1206). We also evaluated possible associations of the polymorphism with insulin sensitivity, beta cell function, with the shape of glucose, insulin and C-peptide trajectories measured 7 times during a 3-hour oral glucose tolerance test (OGTT) and with glucagon response. In a subgroup of 268 volunteers we also evaluated sleep patterns and biorhythm.

Results

13 persons were diagnosed with T2DM, 119 had impaired fasting blood glucose (IFG) and/or impaired glucose tolerance (IGT). 1074 participants showed normal results and formed a control group. A higher frequency of minor allele G was found in the IFG/IGT group in comparison with controls. The GG constellation was present in 23% of diabetics, in 17% of IFG/IGT probands and in 11% of controls. Compared to CC and CG genotypes, GG homozygotes showed higher stimulated glycemia levels during the OGTT. Homozygous as well as heterozygous carriers of the G allele showed lower very early phase of insulin and C-peptide secretion with unchanged insulin sensitivity. These differences remained significant after excluding diabetics and the IFG/IGT group from the analysis. No associations of the genotype with the shape of OGTT-based trajectories, with glucagon or with chronobiological patterns were observed. However, the shape of the trajectories differed significantly between men and women.

Conclusion

In a representative sample of the Czech population, the G allele of the rs10830963 polymorphism is associated with impaired early phase of beta cell function, and this is evident even in healthy individuals.
The Immunoregulatory Actions of DHEA in Tuberculosis, A Tool for Therapeutic Intervention?
Authors: Bongiovanni Bettina , Díaz Ariana , Santucci Natalia , D’Attilio Luciano David , Bottasso Oscar , Hernández Pando Rogelio , Bay María Luisa
Published: June 2022

Dehydroepiandrosterone (DHEA) is an androgen synthesized by the adrenal cortex, which is an intermediary in the biosynthesis of sex hormones, such as testosterone and estradiol. DHEA mostly circulates as a conjugated ester, in the form of sulfate (DHEA-S). There exist several endogenous factors able to influence its synthesis, the most common ones being the corticotrophin-releasing hormone (CRH), adrenocorticotrophin (ACTH), growth factors, and proinflammatory cytokines, among others. Like other steroid hormones, DHEA, can alter the functioning of immune cells and therefore the course of diseases exhibiting an immune-inflammatory component, mostly from autoimmune or infectious nature. We herein review the role played by DHEA during a major infectious disease like tuberculosis (TB). Data recorded from TB patients, mouse models, or in vitro studies show that DHEA is likely to be implied in better disease control. This provides a stimulating background for carrying out clinical studies aimed at assessing the usefulness of DHEA as an adjuvant in TB patients.
Prevention of Hypomineralization In Auditory Ossicles of Vitamin D Receptor (Vdr) Deficient Mice
Authors: Delsmann Maximilian M. , Peichl Jonathan , Yorgan Timur A. , Beil Frank Timo , Amling Michael , Demay Marie B. , Rolvien Tim
Published: June 2022

Intact mineralization of the auditory ossicles - the smallest bones in the body - is essential for sound transmission in the middle ear, while ossicular hypomineralization is associated with conductive hearing loss. Here, we performed a high-resolution analysis of the ossicles in vitamin D receptor deficient mice (Vdr-/-), which are characterized by hypocalcemia and skeletal mineralization defects, and investigated whether local hypomineralization can be prevented by feeding a calcium-rich rescue diet (Vdr-/- res). In Vdr-/- mice fed a regular diet (Vdr-/- reg), quantitative backscattered electron imaging (qBEI) revealed an increased void volume (porosity, p<0.0001) along with lower mean calcium content (CaMean, p=0.0008) and higher heterogeneity of mineralization (CaWidth, p=0.003) compared to WT mice. Furthermore, a higher osteoid volume per bone volume (OV/BV; p=0.0002) and a higher osteocyte lacunar area (Lc.Ar; p=0.01) were found in histomorphometric analysis in Vdr-/- reg mice. In Vdr-/- res mice, full rescue of OV/BV and Lc.Ar (both p>0.05 vs. WT) and partial rescue of porosity and CaWidth (p=0.02 and p=0.04 vs. WT) were observed. Compared with Hyp mice, a model of X-linked hypophosphatemic rickets, Vdr-/- reg mice showed a lower osteoid volume in the ossicles (p=0.0002), but similar values in the lumbar spine. These results are consistent with later postnatal impairment of mineral homeostasis in Vdr-/- mice than in Hyp mice, underscoring the importance of intact mineral homeostasis for ossicle mineralization during development. In conclusion, we revealed a distinct phenotype of hypomineralization in the auditory ossicles of Vdr-/- mice that can be partially prevented by a rescue diet. Since a positive effect of a calcium-rich diet on ossicular mineralization was demonstrated, our results open new treatment strategies for conductive hearing loss. Future studies should investigate the impact of improved ossicular mineralization on hearing function.
Research on the Impact of Green Finance Policy on Regional Green Innovation-Based on Evidence From the Pilot Zones for Green Finance Reform and Innovation
Authors: Zhang Chi , Cheng Xinwei , Ma Yanyan
Published: June 2022

To develop green finance and ensure the goal of carbon peaking and carbon neutrality, China set up the pilot zones for green finance reform and innovation in 2017. We empirically tested the policy effect of the pilot zones with data from 2010 to 2019 for prefecture-level cities in China. The study shows that the pilot zones have induced an effect on regional green technology innovation, reflected in the application and acquisition of both green invention patents and green utility patents, and the promotion effect is better for green utility patents than green invention patents, which is supported by the robustness test using PSM-DID. This study provides theoretical support and empirical evidence for evaluating the policy effects of the pilot zones and provides a reference for the differentiated formulation of green financial policies.
RETRACTED: Globally, Freshwater Ecosystems Emit More CO2 Than the Burning of Fossil Fuels
Authors: Pollard Peter C.
Published: June 2022

Freshwater emits substantial volumes of CO2 to the atmosphere. This has largely gone unnoticed in global carbon budgets. My aim was to quantify the CO2 emanating from freshwater from 66° N to 47° S latitudes via in situ bacterial respiration (BR). I determined BR (n = 326) as a function of water temperature. Freshwater is emitting CO2 at a rate of 58.5 Pg C y−1 (six times that of fossil fuel burning). Most is emitted from the Northern Hemisphere. This is because the high northern summer temperatures coincide with most of the world’s freshwater. Diffuse DOC sources, for example dust, may be driving high freshwater BR. However, many sources remain elusive and not individually quantified in the literature. We must include freshwater CO2 emissions in climate models. Identifying, quantifying and managing freshwater’s diffuse sources of Dissolved Organic Carbon (DOC) will hopefully provide us with another opportunity to change our current climate trajectory.
Effect of Polymer Mixtures on Physical-Chemical Properties of Sandy Soil and Plant Growth
Authors: Yang Qingwen , Pei Xiangjun , Fu Cheng
Published: June 2022

Serious desertification problems were found in alpine grassland of the Qinghai-Tibet Plateau, China. The rehabilitation progress of degraded grassland is slow under natural conditions that have low rainfall and large evaporation. Organic agent-solidified soil is one of the most important measurements that limit desertification and grassland conservation-restoration. However, the characteristics of vegetation growth and development in solidified soil need to be studied in depth. This research is based on the modified carboxymethyl cellulose (MCMC) of independent development. Based on the sand soil taken from moving sandy land of Zoige, potted plants were tested in the environmental chamber at the laboratory. The physical, chemical, and biological characteristics of root soil were monitored for more than 7 months. As a result, the plant biomass in the experimental group increased, lodging rate decreased, drought tolerance and survival rate increased, and the number of plant roots and root surface area index, root cross-sectional area ratio, and root volume ratio increased compared with the matched group. It is shown that MCMC can effectively promote plant root development and improve plant drought tolerance and lodging resistance. We also found that, compared with the matched group, the values of the rhizosphere soil mass, root soil mass, and soil crust layer thickness of the experimental group were much higher, meaning that MCMC has strengthened the root soil-fixation ability and soil-crusting ability of plants. The soil nutrient indexes and bioactivity of the experimental group were higher than the matched group, indicating that MCMC has a positive influence on soil maturation. The quantitative description model of soil aging enhancement of MCMC material during root growth and development was established to explore the soil-fixation mechanism of MCMC plant root succession and provided an important scientific basis and technical support for the conservation and restoration of alpine grassland desertification grassland.
Identification of the Immune Subtype of Hepatocellular Carcinoma for the Prediction of Disease-Free Survival Time and Prevention of Recurrence by Integrated Analysis of Bulk- and Single-Cell RNA Sequencing Data
Authors: Fu Jie , Lei Xiaohua
Published: June 2022

Background

The main factors affecting the long-term prognosis of hepatocellular carcinoma (HCC) patients undergoing radical surgery are recurrence and metastasis. However, the methods for predicting disease-free survival (DFS) time and preventing postoperative recurrence of HCC are still very limited.

Methods

In this study, immune cell abundances in HCC samples were analyzed by single-sample gene set enrichment analysis (ssGSEA), while the prognostic values of immune cells for DFS time prediction were evaluated by the least absolute shrinkage and selection operator (LASSO) and subsequent univariate and multivariate Cox analyses. Next, a risk score was constructed based on the most prognostic immune cells and their corresponding coefficients. Interactions among prognostic immune cells and the specific targets for the prevention of recurrence were further identified by single-cell RNA (scRNA) sequencing data and CellMiner.

Results

A novel efficient T cell risk score (TCRS) was constructed based on data from the three most prognostic immune cell types (effector memory CD8 T cells, regulatory T cells and follicular helper T cells) for identifying an immune subtype of HCC patients with longer DFS times and inflammatory immune characteristics. Functional differences between the high- and low-score groups separated by TCRS were clarified, and the cell-cell communication among these immune cells was elucidated. Finally, fifteen hub genes that may be potential therapeutic targets for the prevention of recurrence were identified.

Conclusions

We constructed and verified a useful model for the prediction of DFS time of HCC after surgery. In addition, fifteen hub genes were identified as candidates for the prevention of recurrence, and a preliminarily investigation of potential drugs targeting these hub genes was carried out.
Transcriptional Pattern Analysis of Virus-Specific CD8+ T Cells in Hepatitis C Infection: Increased Expression of TOX and Eomesodermin During and After Persistent Antigen Recognition
Authors: Wildner Nils H. , Walker Andreas , Brauneck Franziska , Ditt Vanessa , Peine Sven , Huber Samuel , Haag Friedrich , Beisel Claudia , Timm Joerg , Schulze zur Wiesch Julian
Published: June 2022

Thymocyte selection-associated high mobility group box (TOX) has been described to be a key regulator in the formation of CD8+ T cell exhaustion. Hepatitis C virus (HCV) infection with different lengths of antigen exposure in acute, chronic, and after resolution of HCV infection is the ideal immunological model to study the expression of TOX in HCV-specific CD8+ T cells with different exposure to antigen. HCV-specific CD8+ T cells from 35 HLA-A*01:01, HLA-A*02:01, and HLA-A*24:02 positive patients were analyzed with a 16-color FACS-panel evaluating the surface expression of lineage markers (CD3, CD8), ectoenzymes (CD39, CD73), markers of differentiation (CD45RO, CCR7, CD127), and markers of exhaustion and activation (TIGIT, PD-1, KLRG1, CD226) and transcription factors (TOX, Eomesodermin, T-bet). Here, we defined on-target T cells as T cells against epitopes without escape mutations and off-target T cells as those against a “historical” antigen mutated in the autologous sequence. TOX+HCV-specific CD8+ T cells from patients with chronic HCV and on-target T cells displayed co-expression of Eomesodermin and were associated with the formation of terminally exhausted CD127-PD1hi, CD39hi, CD73low CD8+ T cells. In contrast, TOX+HCV-specific CD8+ T cells in patients with off-target T cells represented a progenitor memory Tex phenotype characterized by CD127hi expression and a CD39low and CD73hi phenotype. TOX+HCV-specified CD8+ T cells in patients with a sustained virologic response were characterized by a memory phenotype (CD127+, CD73hi) and co-expression of immune checkpoints and Eomesodermin, indicating a key structure in priming of HCV-specific CD8+ T cells in the chronic stage, which persisted as a residual after therapy. Overall, the occurrence of TOX+HCV-specific CD8+ T cells was revealed at each disease stage, which impacted the development of progenitor Tex, intermediate Tex, and terminally exhausted T cell through an individual molecular footprint. In sum, TOX is induced early during acute infection but is modulated by changes in viral sequence and antigen recognition. In the case of antigen persistence, the interaction with Eomesodermin leads to the formation of terminally exhausted virus-specific CD8+ T cells, and there was a direct correlation of the co-expression of TOX and Eomes and terminally exhausted phenotype of virus-specific CD8+ T cells.
Lung Ultrasound Signs and Their Correlation With Clinical Symptoms in COVID-19 Pregnant Women: The “PINK-CO” Observational Study
Authors: Vetrugno Luigi , Sala Alessia , Orso Daniele , Meroi Francesco , Fabbro Sebastiano , Boero Enrico , Valent Francesca , Cammarota Gianmaria , Restaino Stefano , Vizzielli Giuseppe , Girometti Rossano , Merelli Maria , Tascini Carlo , Bove Tiziana , Driul Lorenza ,   The PINK-CO study investigators
Published: June 2022

Objective

To analyze the application of lung ultrasound (LUS) diagnostic approach in obstetric patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and compare LUS score and symptoms of the patients.

Design

A single-center observational retrospective study from October 31, 2020 to March 31, 2021.

Setting

Department of Ob/Gyn at the University-Hospital of Udine, Italy.

Participants

Pregnant women with SARS-CoV-2 diagnosed with reverse transcription-PCR (RT-PCR) swab test were subdivided as symptomatic and asymptomatic patients with COVID-19.

Exposure

Lung ultrasound evaluation both through initial evaluation upon admission and through serial evaluations.

Main Outcome

Reporting LUS findings and LUS score characteristics.

Results

Symptomatic patients with COVID-19 showed a higher LUS (median 3.5 vs. 0, p < 0.001). LUS was significantly correlated with COVID-19 biomarkers as C-reactive protein (CPR; p = 0.011), interleukin-6 (p = 0.013), and pro-adrenomedullin (p = 0.02), and inversely related to arterial oxygen saturation (p = 0.004). The most frequent ultrasound findings were focal B lines (14 vs. 2) and the light beam (9 vs. 0).

Conclusion

Lung ultrasound can help to manage pregnant women with SARS-CoV-2 infection during a pandemic surge.

Study Registration

ClinicalTrials.gov, NCT04823234. Registered on March 29, 2021.