bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Analysis of Exosomal MicroRNAs Derived from UVB-Irradiated Keratinocytes as Potential Melanogenesis Regulators
Authors: Yoon Jee-Hoe , Jo Chan-Song , Hwang Jae-Sung
Published: March 2024

The exosomes derived from keratinocytes can have a substantial impact on melanogenesis by influencing melanocytes. MicroRNAs (miRNAs) encapsulated within exosomes are implicated in the control of melanogenesis, particularly when under the influence of UVB irradiation. This investigation explores UVB-induced exosomal miRNAs from keratinocytes as potential regulators of melanogenesis. UVB-irradiated, keratinocyte-derived exosomes were observed to augment melanogenesis in melanocytes, resulting in an upregulation of MITF, TRP1, TRP2, and TYR expression compared to non-UVB-irradiated exosomes. Additionally, a subset of exosomal miRNAs was differentially selected and confirmed to exert both enhancing and inhibitory effects on melanogenesis through functional assays. Notably, hsa-miR-644a, hsa-miR-365b-5p, and hsa-miR-29c-3p were found to upregulate melanogenesis, while hsa-miR-18a-5p, hsa-miR-197-5p, and hsa-miR-4281 downregulated melanogenesis. These findings suggest the involvement of keratinocyte-derived exosomal miRNAs in melanogenesis regulation within melanocytes. The expression levels of exosomal miRNAs from keratinocytes exhibited a UVB-dependent increase, indicating a potential role for these miRNAs as regulators of melanogenesis in response to UVB irradiation. Furthermore, melanogenesis was found to be dependent on exosomes derived from keratinocytes. This underscores the potential of UVB-induced exosomal miRNAs derived from keratinocytes as regulators of melanogenesis. Moreover, this study unveils a significant role for exosomes in melanocyte pigmentation, presenting a novel pathway in the intricate process of melanogenesis.
Unveiling the Role of Protein Kinase C θ in Porcine Epidemic Diarrhea Virus Replication: Insights from Genome-Wide CRISPR/Cas9 Library Screening
Authors: Zhou Jinglin , Feng Zhihua , Lv Deyang , Wang Duokai , Sang Kai , Liu Zhihao , Guo Dong , Shen Yangkun , Chen Qi
Published: March 2024

Porcine epidemic diarrhea virus (PEDV), a member of the Alpha-coronavirus genus in the Coronaviridae family, induces acute diarrhea, vomiting, and dehydration in neonatal piglets. This study aimed to investigate the genetic dependencies of PEDV and identify potential therapeutic targets by using a single-guide RNA (sgRNA) lentiviral library to screen host factors required for PEDV infection. Protein kinase C θ (PKCθ), a calcium-independent member of the PKC family localized in the cell membrane, was found to be a crucial host factor in PEDV infection. The investigation of PEDV infection was limited in Vero and porcine epithelial cell-jejunum 2 (IPEC-J2) due to defective interferon production in Vero and the poor replication of PEDV in IPEC-J2. Therefore, identifying suitable cells for PEDV investigation is crucial. The findings of this study reveal that human embryonic kidney (HEK) 293T and L929 cells, but not Vero and IPEC-J2 cells, were suitable for investigating PEDV infection. PKCθ played a significant role in endocytosis and the replication of PEDV, and PEDV regulated the expression and phosphorylation of PKCθ. Apoptosis was found to be involved in PEDV replication, as the virus activated the PKCθ-B-cell lymphoma 2 (BCL-2) ovarian killer (BOK) axis in HEK293T and L929 cells to increase viral endocytosis and replication via mitochondrial apoptosis. This study demonstrated the suitability of HEK293T and L929 cells for investigating PEDV infection and identified PKCθ as a host factor essential for PEDV infection. These findings provide valuable insights for the development of strategies and drug targets for PEDV infection.
Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)
Authors: Uzelac Iva , Avramov Miloš , Knežić Teodora , Tatić Vanja , Gošić-Dondo Snežana , Popović Željko D.
Published: March 2024

During diapause, a state of temporarily arrested development, insects require low winter temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness. A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated the combined effects of thermal stress and the diapause program on the expression of selected genes involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis. By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was strongly controlled by the diapause program and refractory to heat stress, as was the case for mtn and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae and pupae of this important corn pest.
The Effect of HMGB1 and HMGB2 on Transcriptional Regulation Differs in Neuroendocrine and Adenocarcinoma Models of Prostate Cancer
Authors: Salamini-Montemurri Martín , Vizoso-Vázquez Ángel , Barreiro-Alonso Aida , Lorenzo-Catoira Lidia , Rodríguez-Belmonte Esther , Cerdán María-Esperanza , Lamas-Maceiras Mónica
Published: March 2024

Human high-mobility group-B (HMGB) proteins regulate gene expression in prostate cancer (PCa), a leading cause of oncological death in men. Their role in aggressive PCa cancers, which do not respond to hormonal treatment, was analyzed. The effects of HMGB1 and HMGB2 silencing upon the expression of genes previously related to PCa were studied in the PCa cell line PC-3 (selected as a small cell neuroendocrine carcinoma, SCNC, PCa model not responding to hormonal treatment). A total of 72% of genes analyzed, using pre-designed primer panels, were affected. HMGB1 behaved mostly as a repressor, but HMGB2 as an activator. Changes in SERPINE1, CDK1, ZWINT, and FN1 expression were validated using qRT-PCR after HMGB1 silencing or overexpression in PC-3 and LNCaP (selected as an adenocarcinoma model of PCa responding to hormonal treatment) cell lines. Similarly, the regulatory role of HMGB2 upon SERPINE1, ZWINT, FN1, IGFPB3, and TYMS expression was validated, finding differences between cell lines. The correlation between the expression of HMGB1, HMGB2, and their targets was analyzed in PCa patient samples and also in PCa subgroups, classified as neuroendocrine positive or negative, in public databases. These results allow a better understanding of the role of HMGB proteins in PCa and contribute to find specific biomarkers for aggressive PCa.
Zosuquidar: An Effective Molecule for Intracellular Ca2+ Measurement in P-gp Positive Cells
Authors: Pelegrinova Livia , Sofrankova Lucia , Spaldova Jana , Stefik Pavol , Sulova Zdena , Breier Albert , Elefantova Katarina
Published: March 2024

Intracellular calcium, as a second messenger, is involved in multilevel cellular regulatory pathways and plays a role (among other processes) in switching between survival and initiation of cell death in neoplastic cells. The development of multidrug resistance (MDR) in neoplastic cells is associated with the ability of cells to escape programmed cell death, in which dysregulation of intracellular calcium may play an important role. Therefore, reliable monitoring of intracellular calcium levels is necessary. However, such a role might be limited by a real obstacle since several fluorescent intracellular calcium indicators are substrates of membrane ABC drug transporters. For example, Fluo-3/AM is a substrate of P-glycoprotein (ABCB1 member of the ABC family), whose overexpression is the most frequent cause of MDR. The overexpression of ABCB1 prevents MDR cell variants from retaining this tracer in the intracellular space where it is supposed to detect calcium. The solution is to use a proper inhibitor of P-gp efflux activity to ensure the retention of the tracer inside the cells. The present study showed that Zosuquidar and Tariquidar (P-gp inhibitors) are suitable for monitoring intracellular calcium, either by flow cytometry or confocal microscopy, in cells overexpressing P-gp.
Polyallylamine Binds to Aβ Amyloid and Inhibits Antibody Recognition
Authors: Tsuchie Yusuke , Kusuda Soichiro , Kawabe Haruka , Mori Wakako , Lindgren Mikael , Watanabe Yutaka , Zako Tamotsu
Published: March 2024

Protein amyloids have attracted attention for their application as functional amyloid materials because of their strong properties, such as high resistance to chemical or biological degradation, despite their medical issues. Amyloids can be used for various applications by modifying the amyloid surface with functional materials, such as proteins and polymers. In this study, we investigated the effect of polyallylamine (PAA), a functional cationic polymer as a candidate for amyloid modification, on the amyloids formed from amyloid β (Aβ) peptide. It was demonstrated for the first time that PAA can bind to Aβ amyloids through fluorescence observations and the quenched emission from the tyrosine at site 10 near the fibrillogenic core. These results suggest that PAA could be used to develop new functional amyloids. However, notably, coating Aβ amyloid with PAA could affect conventional amyloid detection assays such as thioflavin T assay and detection using antibodies. Thus, our results also indicate that consideration would be necessary for the analysis of functional amyloids coated with various polymers.
Sustained Disease Control in DME Patients upon Treatment Cessation with Brolucizumab
Authors: Garweg Justus G. , Steinhauer Sonja
Published: March 2024

Background: Treatment cessation due to a dry retina has not been systematically addressed in diabetic macular edema (DME). In three out of four patients receiving 6 mg of brolucizumab in the KITE study, treatment was terminated after the study ended. Methods: The KITE study was a double-masked, multicenter, active-controlled, randomized trial (NCT 03481660) in DME patients. Per protocol, patients received five loading injections of Brolucizumab at 6-week intervals, with the option to adjust to 8 weeks in case of disease activity or to extend in the second year to a maximum of 16 weeks in the absence of retinal fluid. Results: After two years, one patient required eight weekly injections, while three patients reached a maximal treatment interval of 16 weeks. The severity of diabetic retinopathy improved in all patients with no dye leakage according to fluorescein angiography (FA) and no retinal fluid according to OCT in three patients. Treatment was paused in these three patients for >36 months, while the fourth patient required continuous treatment at 5-week intervals after switching to other licensed anti-VEGF agents. Conclusions: The adoption of treatment according to individual needs, including considering treatment cessation, may contribute to improved treatment adherence in many patients and be more frequently possible than expected.
Association between Ustekinumab Trough Levels, Serum IL-22, and Oncostatin M Levels and Clinical and Biochemical Outcomes in Patients with Crohn’s Disease
Authors: Bertin Luisa , Barberio Brigida , Gubbiotti Alessandro , Bertani Lorenzo , Costa Francesco , Ceccarelli Linda , Visaggi Pierfrancesco , Bodini Giorgia , Pasta Andrea , Sablich Renato , Urbano Maria Teresa , Ferronato Antonio , Buda Andrea , De Bona Manuela , Del Corso Giulio , Massano Alessandro , Angriman Imerio , Scarpa Marco , Zingone Fabiana , Savarino Edoardo Vincenzo
Published: March 2024

Background: Ustekinumab (UST) has demonstrated effectiveness in treating patients with Crohn’s disease. Monitoring treatment response can improve disease management and reduce healthcare costs. We investigated whether UST trough levels (TLs), serum IL22, and Oncostatin M (OSM) levels could be early indicators of non-response by analysing their correlation with clinical and biochemical outcomes in CD. Methods: Patients with CD initiating UST treatment from October 2018 to September 2020 were enrolled at six Italian centres for inflammatory bowel disease (IBD). Clinical and biochemical data were collected at four time points: baseline, second subcutaneous (SC) dose, fourth SC dose, and 52 weeks. TLs were measured during maintenance, at the second SC dose, and at the fourth SC dose. IL-22 and OSM serum levels were assessed at baseline and the second SC dose. We analysed whether TLs, IL22 levels, and OSM serum levels were associated with clinical response, clinical remission, biochemical remission, and endoscopic remission using the appropriate statistical tests. Results: Out of eighty-four initially enrolled patients, five were lost to follow-up, and eleven discontinued the drug before 52 weeks. At the 52-week time point, 47% achieved biochemical remission based on faecal calprotectin levels, and 61.8% achieved clinical remission. TLs at the second SC dose significantly correlated with biochemical remission at the same time point (p = 0.011). However, TLs did not correlate with clinical remission. Baseline OSM levels did not correlate with biochemical or clinical remission or response. IL22 levels notably decreased during UST therapy (p = 0.000), but its values did not correlate with biochemical or clinical remission. Conclusions: UST is an effective therapy for patients with CD. TLs measured at the second SC dose significantly correlated with biochemical remission, emphasising their potential role in treatment monitoring. Levels of OSM and IL-22, despite a significant decrease in the latter during therapy, did not exhibit correlations with clinical or biochemical outcomes in our study. Further studies are needed to confirm these findings.
Studies on the Mechanical, Strengthening Mechanisms and Tribological Characteristics of AA7150-Al2O3 Nano-Metal Matrix Composites
Authors: Maddaiah K. Chinna , Kumar G. B. Veeresh , Pramod R.
Published: March 2024

Stir-casting with ultrasonic cavitation produced nano-Al2O3-filled AA7150 matrix composites in this study. The SEM microstructure study shows that all composites include nano-Al2O3 particles with consistent particle sizes and homogenous distribution. EDS and XRD showed no secondary phases or impurities in the composite. Optical microscopy showed intense ultrasonic cavitation effects, and nano-Al2O3 particles caused grain refinement in the AA7150 matrix. The composite’s mechanical characteristics improved when the Al2O3 nanoparticle weight percentage (wt.%) increased. With only 2.0 wt.% nano-Al2O3 particles, the composites yielded 232 MPa, 97.52% higher than the sonicated AA7150 matrix alloy. Multiple models were used to characterize the strength of the AA7150 nano-Al2O3 composite. The findings showed that thermal incongruity, Orowan strengthening, the Hall–Petch mechanism, and load transfer effects contributed the most towards the increased strength of the composite. Increasing the nano-Al2O3 wt.% in the AA7150 matrix improved hardness by 95.08%, yield strength by 90.34%, and sliding wear resistance by 46.52%. This enhancement may be attributed to the combined effects of better grain refinement, enhanced dispersion with dislocation strengthening, and better load transfer between the matrix and reinforcement, which are assisted by the inclusion of reinforcements. This result was confirmed by optical studies.
The Potential Teeth Bleaching and Halitosis Prevention Effects of Pediococcus inopinatus THK-30, a Kimchi-Derived Lactic Acid Bacterium: In Vitro Study
Authors: Nguyen Trang Thi Minh , Zheng Qiwen , Yi Eun-Ji , Bellere Arce Defeo , Jin Xiangji , Kim Hong-Yong , Yi Tae-Hoo
Published: March 2024

Background: Recent developments in addressing dental aesthetic concerns, encompassing issues like teeth discoloration and halitosis, underscore the demand for safer alternative solutions. Purpose: This study aims to confirm the effects of lactic acid bacteria (LAB) from kimchi on artificial teeth bleaching and their potential impact in terms of preventing halitosis-related bacteria. Materials and Methods: To evaluate the antimicrobial effects against oral pathogens, disc diffusion tests and broth microdilution methods were used. Additionally, crystal violet analysis was performed to confirm the biofilm inhibition effect. The bleaching effects on stained artificial teeth were analyzed using the CIEDE2000 colorimetric method. Statistical analyses were performed using GraphPad Prism 9 with one-way and two-way ANOVA, with the significance level set at α < 0.05. Results: The strain THK-30, isolated from kimchi, exhibited antibacterial activity against Streptococcus mutans, Porphyromonas gingivalis, and Fusobacterium nucleatum, and was identified as Pediococcus inopinatus. Moreover, THK-30 showed a synergistic antibacterial effect against Gram-negative oral pathogens with 8% sodium hexametaphosphate (SHMP). In the stained artificial teeth bleaching test and artificial teeth biofilm inhibition test, the cell-free supernatant of THK-30 displayed significant teeth bleaching effects and caused the inhibition of biofilm formation, both independently and in combination with SHMP 8%. Conclusions: This study has demonstrated the potential applicability of LAB in teeth discoloration and halitosis. These findings are poised to provide a foundation for the development of research pertaining to the control of oral bacteria.
Revealing the Spatial-Temporal Evolution and Obstacles of Ecological Security in the Xiamen-Zhangzhou-Quanzhou Region, China
Authors: Zhang Yaping , Zhang Jianjun , Li Yufei , Liang Sen , Chen Wei , Dai Yixin
Published: March 2024

Climate change and human activities have caused various ecological risks to coastal urban agglomerations. Ecological security refers to the state of health of an ecosystem and its integrity. An objective and comprehensive evaluation of ecological security is significant for protecting the structure and function of coastal ecosystems. The driving force–pressure–state–impact–response (DPSIR) model was used to construct a dynamic simulation model of ecological security in the Xiamen–Zhangzhou–Quanzhou region (XZQR), located on the eastern coast of China. The ecological security level (ESL) characteristics of the spatial and temporal patterns were evaluated by calculating the ecological security index (ESI). Obstacle factors were analyzed as well. The results show the following: (1) From 2011 to 2021, the average ESI rose from 0.238 to 0.686 and went through a relatively insecure stage (2011–2015), a critical stage (2016–2019), and a relatively secure stage (2020–2021). (2) The ESI level in Quanzhou was higher in the early stage, and the level of ecological security in Zhangzhou showed a significant rising trend, increasing by 0.541. Its increase depended on increases in the impact layer. (3) The impact layer is the main obstacle layer affecting the ESL, and the main obstacles include CO2 emissions (0.117), annual rainfall (0.091), general public budget expenditures (0.082), GDP growth rates (0.082), and green coverage in built-up areas (0.075). Therefore, we recommend promoting the complementary advantages of the XZQR and implementing ecological restoration projects.
Green Transition Assessment, Spatial Correlation, and Obstacles Identification: Evidence from Urban Governance Data of 288 Cities in China
Authors: Yu Ziao , Guo Tianjiao , Song Xiaoqian , Zhang Lifan , Cai Linmei , Zhang Xi , Zhao Aiwen
Published: March 2024

The green transition of China’s cities is crucial for ecology civilization realization. Based on the driver–pressure–state–impact–response (DPSIR) framework, an integrated technique for order preference by similarity to ideal solution (TOPSIS) model with entropy weight, this study achieved the comprehensive assessment of the green transition of 288 province-level municipalities and prefecture-level cities in China over 18 years from 2002 to 2019, in addition to the spatial correlations and obstacles analysis. The results indicate that major cities in China have a more significant green transition value, and the eastern region is developing fast, while the northeast region is relatively slow. There was heterogeneous spatial distribution for green transition, because of the disequilibrium sustainable development of 288 cities. Green transition has a significantly positive spatial autocorrelation in the cities of China, the high–high significant clusters greatly increased, and the main locations changed from the northeast to southeast of China. Frequent obstacles were also found, including road infrastructure construction, water resources, and the green coverage of urban built-up areas. Based on these results, several policy implications were put forward, including the optimization of environmental laws and regulations, the development of green transportation infrastructure, resource conservation and the circular economy, the establishment of a green financial system, and increasing the linkage for the green transition of different cities.
Properties of Cementitious Materials Utilizing Seashells as Aggregate or Cement: Prospects and Challenges
Authors: Zhu Yunpeng , Chen Da , Yu Xiaotong , Liu Ruiwen , Liao Yingdi
Published: March 2024

Nowadays, the sustainable development of the construction industry has become a focus of attention. Crushing and grinding waste seashells originating from the fishery industry, such as oyster shells, cockle shells, mussel shells, and scallop shells, into different particle sizes for usage as aggregate and cement in concrete or mortar provides an effective and sustainable solution to environmental problems by reducing natural resource dependence. Numerous studies have attempted to analyze the suitability of waste seashell as a possible alternative to natural aggregates and cement in concrete or mortar. This paper presents an up-to-date review of the characteristics of different types of waste seashell, as well as the physical, mechanical, durability, and other notable functional properties of seashell concrete or mortar. From the outcome of the research, waste seashell could be an inert material, and it is important to conduct a series of proper treatment for a better-quality material. It is also seen from the results that although the mechanical properties of seashell concrete have been reduced, they all meet the required criteria set by various international standards and codes. Therefore, it is recommended that the replacement of seashells as aggregate and cement should not exceed 20% and 5%, respectively. Seashell concrete or mortar would then have sufficient workability and strength for non-structural purposes. However, there is still a lack of investigation concerning the different properties of reinforced concrete members using seashells as the replacement of aggregate or cement. Further innovative research can solidify its utilization towards sustainable development.
Biocomposite Materials Derived from Andropogon halepensis: Eco-Design and Biophysical Evaluation
Authors: Barbinta-Patrascu Marcela-Elisabeta , Nichita Cornelia , Bita Bogdan , Antohe Stefan
Published: March 2024

This research work presents a “green” strategy of weed valorization for developing silver nanoparticles (AgNPs) with promising interesting applications. Two types of AgNPs were phyto-synthesized using an aqueous leaf extract of the weed Andropogon halepensis L. Phyto-manufacturing of AgNPs was achieved by two bio-reactions, in which the volume ratio of (phyto-extract)/(silver salt solution) was varied. The size and physical stability of Andropogon—AgNPs were evaluated by means of DLS and zeta potential measurements, respectively. The phyto-developed nanoparticles presented good free radicals-scavenging properties (investigated via a chemiluminescence technique) and also urease inhibitory activity (evaluated using the conductometric method). Andropogon—AgNPs could be promising candidates for various bio-applications, such as acting as an antioxidant coating for the development of multifunctional materials. Thus, the Andropogon-derived samples were used to treat spider silk from the spider Pholcus phalangioides, and then, the obtained “green” materials were characterized by spectral (UV-Vis absorption, FTIR ATR, and EDX) and morphological (SEM) analyses. These results could be exploited to design novel bioactive materials with applications in the biomedical field.
Correction: Zhang et al. Dynamic Multi-Objective Optimization in Brazier-Type Gasification and Carbonization Furnace. Materials 2023, 16, 1164
Authors: Zhang Xi , Zhang Guiyun , Zhang Dong , Zhang Liping
Published: March 2024

In consideration of the contributions to this work, Feng Qian unequivocally requests the removal of his name from the author list of this publication [...]
The Study of Humic Substances’ Impact on Anion Exchangers
Authors: Wiercik Paweł , Garbowski Tomasz , Chrobot Przemysław
Published: March 2024

Humic substances (HSs) present in water and wastewater cause fouling of anion exchange resins (AERs), which mainly results in reducing the ion exchange capacity (IEC). In this paper, an attempt was made to investigate fouling of two polystyrene and one polyacrylic AER using water from the Oder River, treated wastewater after the ultrafiltration process (UFTW) and digester reject water from sludge dewatering at the Janówek Wastewater Treatment Plant (WWTP) in Wrocław. HSs contained in digester reject water were characterised by the lowest aromaticity and molecular weights (MWs), the highest proportion of hydrophilic fraction and the highest amount of oxygenated functional groups. The Fourier-transform infrared (FTIR) analyses made it possible to identify chemical bonds characteristic of HSs and determine the mechanism of their retention on the surface of AER beads. The conducted experiments brought unexpected results, as the IEC increased with the amount of organic matter in the feed. Presumably, the humic substances accumulated on the beads and in the porosity of the anion exchangers themselves participated in the ion exchange process.
Performance Enhancement of Three-Dimensional MAPbI3 Perovskite Solar Cells by Doping Perovskite Films with CsPbX3 Quantum Dots
Authors: Tsai Ming-Chen , Chu Sheng-Yuan , Kao Po-Ching
Published: March 2024

Perovskite thin films directly impact solar cell properties, making defect reduction crucial in perovskite solar cell research. In our study, we used perovskite quantum dots in the anti-solvent to act as nucleation centers in MAPbI3 thin films. These centers had lower nucleation barriers than homogeneous nucleation, improving perovskite crystallinity, reducing defects, and extending carrier lifetime. Fine-tuning the energy band also enhanced carrier transport. The most effective results were obtained using CsPb(Br0.5 I0.5)3 perovskite quantum dots. The resulting device, ITO/SnO2/MAPbI3 (300 nm)/spiro-OMeTAD (200 nm)/Ag (100 nm), achieved a 12.88% power conversion efficiency, a 16% increase from the standard element. The modified device maintained approximately 95% of its efficiency over 100 h in a 70% humidity environment.
Chains with Connections of Diffusion and Advective Types
Authors: Kashchenko Sergey
Published: March 2024

The local dynamics of a system of oscillators with a large number of elements and with diffusive- and advective-type couplings containing a large delay are studied. Critical cases in the problem of the stability of the zero equilibrium state are singled out, and it is shown that all of them have infinite dimensions. Applying special methods of infinite normalization, we construct quasinormal forms, namely, nonlinear boundary value problems of the parabolic type, whose nonlocal dynamics determine the behavior of the solutions of the initial system in a small neighborhood of the equilibrium state. These quasinormal forms contain either two or three spatial variables, which emphasizes the complexity of the dynamical properties of the original problem.
Current Status of Indole-Derived Marine Natural Products: Synthetic Approaches and Therapeutic Applications
Authors: Fernández Sergio , Arnáiz Virginia , Rufo Daniel , Arroyo Yolanda
Published: March 2024

Indole is a versatile pharmacophore widely distributed in bioactive natural products. This privileged scaffold has been found in a variety of molecules isolated from marine organisms such as algae and sponges. Among these, indole alkaloids represent one of the biggest, most promising family of compounds, having shown a wide range of pharmacological properties including anti-inflammatory, antiviral, and anticancer activities. The aim of this review is to show the current scenario of marine indole alkaloid derivatives, covering not only the most common chemical structures but also their promising therapeutic applications as well as the new general synthetic routes developed during the last years.
Consequences of the Direct Reduction and Electric Steelmaking Grid Creation on the Italian Steel Sector
Authors: Dall’Osto Gianluca , Mombelli Davide , Mapelli Carlo
Published: March 2024

The consequences on the Italian steel sector following the conversion of the sole integrated steel plant and the establishment of a direct reduction/electric arc furnace (DR/EAF) grid in the period 2022–2050 were analyzed. Imported natural gas (pathway 0), green hydrogen (pathway 1) and biomethane (pathway 2) were studied as possible reducing gases to be exploited in the DR plant and to be introduced as a methane substitute in EAFs. The results showed that the environmental targets for the sustainable development scenario could be achieved in both 2030 and 2050. In particular, the main reduction would occur by 2030 as a result of the cease of the integrated plant itself, allowing for an overall reduction of 71% of the CO2 emitted in 2022. On the other hand, reaching the maximum production capacity of the DR plants by 2050 (6 Mton) would result in final emission reductions of 25%, 80% and 35% for pathways 0, 1 and 2, respectively. Finally, the creation of a DR/EAF grid would increase the energy demand burden, especially for pathway 1, which would require three times as much green energy as pathway 0 and/or 2 (36 TWh/y vs. ca. 12 TWh/y).
Daily Injection of the β2 Adrenergic Agonist Clenbuterol Improved Muscle Glucose Metabolism, Glucose-Stimulated Insulin Secretion, and Hyperlipidemia in Juvenile Lambs Following Heat-Stress-Induced Intrauterine Growth Restriction
Authors: Gibbs Rachel L. , Wilson James A. , Swanson Rebecca M. , Beard Joslyn K. , Hicks Zena M. , Beer Haley N. , Marks-Nelson Eileen S. , Schmidt Ty B. , Petersen Jessica L. , Yates Dustin T.
Published: March 2024

Stress-induced fetal programming diminishes β2 adrenergic tone, which coincides with intrauterine growth restriction (IUGR) and lifelong metabolic dysfunction. We determined if stimulating β2 adrenergic activity in IUGR-born lambs would improve metabolic outcomes. IUGR lambs that received daily injections of saline or the β2 agonist clenbuterol from birth to 60 days were compared with controls from pair-fed thermoneutral pregnancies. As juveniles, IUGR lambs exhibited systemic inflammation and robust metabolic dysfunction, including greater (p < 0.05) circulating TNFα, IL-6, and non-esterified fatty acids, increased (p < 0.05) intramuscular glycogen, reduced (p < 0.05) circulating IGF-1, hindlimb blood flow, glucose-stimulated insulin secretion, and muscle glucose oxidation. Daily clenbuterol fully recovered (p < 0.05) circulating TNFα, IL-6, and non-esterified fatty acids, hindlimb blood flow, muscle glucose oxidation, and intramuscular glycogen. Glucose-stimulated insulin secretion was partially recovered (p < 0.05) in clenbuterol-treated IUGR lambs, but circulating IGF-1 was not improved. Circulating triglycerides and HDL cholesterol were elevated (p < 0.05) in clenbuterol-treated IUGR lambs, despite being normal in untreated IUGR lambs. We conclude that deficient β2 adrenergic regulation is a primary mechanism for several components of metabolic dysfunction in IUGR-born offspring and thus represents a potential therapeutic target for improving metabolic outcomes. Moreover, benefits from the β2 agonist were likely complemented by its suppression of IUGR-associated inflammation.
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
Authors: He Jinjian , Li Yuying , Sun Xihang , Zuo Dinghui , Wang Mansheng , Zheng Xia , Yu Pinglian , Shi Pengjun
Published: March 2024

A putative β-glucosidase gene, BglAc, was amplified from Acidilobus sp. through metagenome database sampling from a hot spring in Yellowstone National Park. BglAc is composed of 485 amino acid residues and bioinformatics analysis showed that it belongs to the GH1 family of β-glucosidases. The gene was successfully expressed in Escherichia coli with a molecular weight of approximately 55.3 kDa. The purified recombinant enzyme showed the maximum activity using p-nitrophenyl-β-D-glucopyranoside (pNPG) as the substrate at optimal pH 5.0 and 100 °C. BglAc exhibited extraordinary thermostability, and its half-life at 90 °C was 6 h. The specific activity, Km, Vmax, and Kcat/Km of BglAc toward pNPG were 357.62 U mg−1, 3.41 mM, 474.0 μmol min−1·mg−1, and 122.7 s−1mM−1. BglAc exhibited the characteristic of glucose tolerance, and the inhibition constant Ki was 180.0 mM. Furthermore, a significant ethanol tolerance was observed, retaining 96% relative activity at 10% ethanol, and even 78% at 20% ethanol, suggesting BglAc as a promising enzyme for cellulose saccharification. BglAc also had a strong ability to convert the major soybean isoflavone glycosides (daidzin, genistin, and glycitin) into their corresponding aglycones. Overall, BglAc was actually a new β-glucosidase with excellent thermostability, ethanol tolerance, and glycoside hydrolysis ability, indicating its wide prospects for applications in the food industry, animal feed, and lignocellulosic biomass degradation.
Ruminal Microbiome Differences in Angus Steers with Differing Feed Efficiencies during the Feedlot Finishing Phase
Authors: Carmichael Mia N. , Dycus Madison M. , Lourenco Jeferson M. , Welch Christina B. , Davis Dylan B. , Krause Taylor R. , Rothrock Michael J. , Fluharty Francis L. , Pringle Timothy D. , Callaway Todd R.
Published: March 2024

The catabolic activity of the ruminal microbial community of cattle enables the conversion of low-quality feedstuffs into meat and milk. The rate at which this conversion occurs is termed feed efficiency, which is of crucial importance given that feed expenses account for up to 70% of the cost of animal production. The present study assessed the relationship between cattle feed efficiency and the composition of their ruminal microbial communities during the feedlot finishing period. Angus steers (n = 65) were fed a feedlot finishing diet for 82 days and their growth performance metrics were evaluated. These included the dry matter intake (DMI), average daily gain (ADG), and residual feed intake (RFI). Steers were rank-ordered based upon their RFI, and the five lowest RFI (most efficient) and five highest RFI (least efficient) steers were selected for evaluations. Ruminal fluid samples were collected on days 0 and 82 of the finishing period. Volatile fatty acids (VFA) were quantified, and microbial DNA was extracted and the 16S rRNA gene was sequenced. The results showed that the ADG was not different (p = 0.82) between efficiency groups during the 82-day feedlot period; however, the efficient steers had lower (p = 0.03) DMI and RFI (p = 0.003). Less-efficient (high RFI) steers developed higher (p = 0.01) ruminal Methanobrevibacter relative abundances (p = 0.01) and tended (p = 0.09) to have more Methanosphaera. In high-efficiency steers (low RFI), the relative abundances of Ruminococcaceae increased (p = 0.04) over the 82-day period. The molar proportions of VFA were not different between the two efficiency groups, but some changes in the concentration of specific VFA were observed over time. The results indicated that the ruminal microbial populations of the less-efficient steers contained a greater relative abundance of methanogens compared to the high-efficiency steers during the feedlot phase, likely resulting in more energetic waste in the form or methane and less dietary energy being harvested by the less-efficient animals.
The Relationship between Granitic Magma and Mineralization in the Darongxi Skarn W Deposit, Xiangzhong District, South China: Constrained by Zircon and Apatite
Authors: Cai Lei , Li Wei , Xie Guiqing , Yin Fangyuan
Published: March 2024

The Xiangzhong district is the largest low-temperature W-Au-Sb metallogenic area in the world. The Darongxi skarn W deposit in the north of the Xiangzhong district is closely related to biotite monzonite granite, muscovite monzonite granite, and felsophyre, but the nature of granitic magma and its relationship with mineralization is relatively weak. In this paper, U-Pb dating, Lu-Hf isotope, the in situ composition of zircon, and the apatite of biotite monzonite granite, muscovite monzonite granite, and felsophyre in the Darongxi mining area are systematically studied, and the formation age, magma property and source, and their relationship with mineralization are discussed. The values of zircon U-Pb age and the εHf(t) of biotite monzonite granite are 222.2 ± 0.54 Ma and −2.9~−6.4, respectively. The values of zircon U-Pb age and the εHf(t) of muscovite monzonite granite are 220.8 ± 0.58 Ma and −2.7 to −8.1, respectively. The values of zircon U-Pb age and the εHf(t) of felsophyre are 222.3 ± 2.20 Ma and −2.2~−5.4, respectively. Magmatic apatite grains from biotite monzonite granite and muscovite monzonite granite show distinctive core–rim and oscillatory zoning textures in CL images, and demonstrate a bright yellow in colorful CL images. The magmatic apatite has a total rare earth concentration (3766~4627 ppm), exhibiting right-inclined nomorlized rare earth element patterns and obvious negative Eu anomalies. The geochemical data of magmatic zircon and apatite indicate that magma sources are responsible for these intrusions in the Darongxi mining area, mainly derived from the partial melting of the Mesoproterozoic crust, which is rich in W; the magma is rich in F and poor in Cl (F = 2.4~3.3 wt%, Cl = 0.0024~0.0502 wt%). The oxygen fugacity of magmatic zircon (ΔFMQAVG = −4.02~−0.26), the high negative Eu anomaly (δEu = 0.06~0.12) and the low positive Ce anomaly (δCe = 1.09~1.13) of magmatic apatite, and the occurrence of ilmenite all indicate that the redox condition of magma from the Darongxi mining area is reduced. The reduced F-rich crust-source granitic rock and W-rich source provide favorable conditions for the mineralization of the Darongxi reduced skarn W deposit.
Research Progress of Bioactive Components in Sanghuangporus spp.
Authors: Lu Jungu , Su Manman , Zhou Xuan , Li Deming , Niu Xinhui , Wang Yi
Published: March 2024

The species in Sanghuangporus are a group of edible mushrooms with a long history of oral use in East Asia as a health-improvement method. They should be classified under the genus Sanghuangporus rather than mistakenly in Phellinus or Inonotus. The major components in this genus consist of polysaccharides, polyphenols, triterpenoids, and flavonoids, all of which exist in the fruiting bodies and mycelia. For extraction, studies have shown methods using hot water, ethanol, DES solvent, and alkaline, followed by purification methods including traditional anion column, Sevag solution, macroporous resin, and magnetic polymers. Proven by modern medical technology, these components possess promising anti-inflammatory, antioxidative, antitumor, and immunoregulation effects; additionally, they have health-improving effects including pulmonary protection, hypoglycemic properties, sleep improvement, gout mitigation, antiaging, neuroprotection, and muscle-strengthening abilities. Several toxicity studies have revealed their safety and recommend a dose of 1 g/kg for mice. As a newly emerged concept, functional food can provide not only life-sustaining nutrients but also some health-improving effects. In conclusion, we substantiate Sanghuang as a functional food by comprehensively presenting information on extraction and purification methods, component medical and structural properties, and nontoxicity, hoping to benefit the development of Sanghuang species as a group of functional food.
A Convenient Strategy for the Voltammetric Direct Determination of Ce(III) in Environmental Waters Using a Multi-Walled Carbon Nanotubes Modified Screen-Printed Carbon Electrode
Authors: Grabarczyk Malgorzata , Fialek Marzena , Wlazlowska Edyta
Published: March 2024

A simple and fast stripping voltammetric procedure for trace determination of Ce(III) in environmental water samples has been developed. The procedure of cerium determination in the presence of Alizarin S and acetate buffer was employed as the initial method. The adsorption material, multi-walled carbon nanotubes, was used as a screen-printed electrode modifier ensuring efficient accumulation of the Ce(III)-Alizarin S complex. The calibration graph for Ce(III) for an accumulation time of 60 s was linear in the range from 1 × 10−8 to 7 × 10−7 mol L−1 with the linear correlation coefficient r = 0.997. The detection limit was estimated from three times the standard deviation of low Ce(III) concentration and an accumulation time of 60 s was about 3.5 × 10−9 mol L−1. The proposed method was successfully applied to Ce(III) determination at trace levels in environmental water samples, such as river, lake and rain water with recoveries ranged from 93% to 98%.
The Roles of Exosome-Derived microRNAs in Cardiac Fibrosis
Authors: Tang Xinyuan , Leng Mingyang , Tang Wenyue , Cai Zhenlu , Yang Lin , Wang Liang , Zhang Yue , Guo Jiao
Published: March 2024

Cardiovascular disease (CVD) stands as the foremost cause of patient mortality, and the lack of early diagnosis and defined treatment targets significantly contributes to the suboptimal prevention and management of CVD. Myocardial fibrosis (MF) is not only a complex pathogenic process with no effective treatment currently available but also exerts detrimental effects on the progression of various cardiovascular diseases, thereby escalating their mortality rates. Exosomes are nanoscale biocommunication vehicles that facilitate intercellular communication by transporting bioactive substances, such as nucleic acids and proteins, from specific cell types. Numerous studies have firmly established that microRNAs (miRNAs), as non-coding RNAs, wield post-transcriptional regulatory mechanisms and exhibit close associations with various CVDs, including coronary heart disease (CHD), atrial fibrillation (AF), and heart failure (HF). MiRNAs hold significant promise in the diagnosis and treatment of cardiovascular diseases. In this review, we provide a concise introduction to the biological attributes of exosomes and exosomal miRNAs. We also explore the roles and mechanisms of distinct cell-derived exosomal miRNAs in the context of myocardial fibrosis. These findings underscore the pivotal role of exosomes in the diagnosis and treatment of cardiac fibrosis and emphasize their potential as biotherapies and drug delivery vectors for cardiac fibrosis treatment.
Multi-Layer Palladium Diselenide as a Contact Material for Two-Dimensional Tungsten Diselenide Field-Effect Transistors
Authors: Murastov Gennadiy , Aslam Muhammad Awais , Leitner Simon , Tkachuk Vadym , Plutnarová Iva , Pavlica Egon , Rodriguez Raul D. , Sofer Zdenek , Matković Aleksandar
Published: March 2024

Tungsten diselenide (WSe2) has emerged as a promising ambipolar semiconductor material for field-effect transistors (FETs) due to its unique electronic properties, including a sizeable band gap, high carrier mobility, and remarkable on–off ratio. However, engineering the contacts to WSe2 remains an issue, and high contact barriers prevent the utilization of the full performance in electronic applications. Furthermore, it could be possible to tune the contacts to WSe2 for effective electron or hole injection and consequently pin the threshold voltage to either conduction or valence band. This would be the way to achieve complementary metal–oxide–semiconductor devices without doping of the channel material.This study investigates the behaviour of two-dimensional WSe2 field-effect transistors with multi-layer palladium diselenide (PdSe2) as a contact material. We demonstrate that PdSe2 contacts favour hole injection while preserving the ambipolar nature of the channel material. This consequently yields high-performance p-type WSe2 devices with PdSe2 van der Waals contacts. Further, we explore the tunability of the contact interface by selective laser alteration of the WSe2 under the contacts, enabling pinning of the threshold voltage to the valence band of WSe2, yielding pure p-type operation of the devices.
The Impact of Diet on miRNA Regulation and Its Implications for Health: A Systematic Review
Authors: DeLucas María , Sánchez Juana , Palou Andreu , Serra Francisca
Published: March 2024

The evidence suggests that diet can modulate endogenous microRNA (miRNA) expression. Changes in miRNA expression may affect metabolic processes and consequently be involved in health status and disease development. The aim of this systematic review was to summarize the evidence of the role of diet and specific food components in the regulation of miRNA expression and discuss its implications for human health and disease development. The PubMed, Embase and Web of Science databases were searched in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines for relevant studies. A total of 32 interventional and 5 observational studies performed in adults and evaluating dietary modulation of miRNA expression were included. Energy- and fat-controlled diets along with plant-based foods show substantial evidence of modulating endogenous miRNA levels. Plasma, serum and peripheral blood mononuclear cells (PBMCs) are the main sources used to measure miRNAs. A total of 108 miRNAs modulated by diet were identified. We confirmed that dietary habits are closely associated with the modulation of endogenous miRNAs. Particularly, energy content and fat intake appeared to be key factors influencing miRNA levels. Furthermore, since miRNAs are involved in the regulation of several biological processes, this modulatory process may affect health status and lead to metabolic disorders.
Galleria mellonella as a Model for the Study of Fungal Pathogens: Advantages and Disadvantages
Authors: Giammarino Andrea , Bellucci Nicolò , Angiolella Letizia
Published: March 2024

The study of pathogenicity and virulence of fungal strains, in vivo in the preclinical phase, is carried out through the use of animal models belonging to various classes of mammals (rodents, leproids, etc.). Although animals are functionally more similar to humans, these studies have some limitations in terms of ethics (animal suffering), user-friendliness, cost-effectiveness, timing (physiological response time) and logistics (need for adequately equipped laboratories). A good in vivo model must possess some optimal characteristics to be used, such as rapid growth, small size and short life cycle. For this reason, insects, such as Galleria mellonella (Lepidoptera), Drosophila melanogaster (Diptera) and Bombyx mori (Lepidoptera), have been widely used as alternative non-mammalian models. Due to their simplicity of use and low cost, the larvae of G. mellonella represent an optimal model above all to evaluate the virulence of fungal pathogens and the use of antifungal treatments (either single or in combination with biologically active compounds). A further advantage is also represented by their simple neuronal system limiting the suffering of the animal itself, their ability to survive at near-body ambient temperatures as well as the expression of proteins able to recognise combined pathogens following the three R principles (replacement, refinement and reduction). This review aims to assess the validity as well as the advantages and disadvantages of replacing mammalian classes with G. mellonella as an in vivo study model for preclinical experimentation.