bluuebunny/crossref_metadata_2025 huggingface dataset:
Deep Two-Photon Imaging of The Live Mouse Brain Using an Array of Quantum Detectors
Authors: Tamimi Amr , Caldarola Martin , Noordzij Niels , Los Johannes W. N. , Guardiani Antonio , Kooiman Hugo , Boffi Juan Carlos , Hambura Sebastian , Wang Ling , Kieser Christian , Fognini Andreas , Prevedel Robert
Published: June 2024

We developed an array of quantum detectors to allow two-photon excited fluorescence in-vivo imaging of mouse brain vasculature in the short wave infrared (SWIR) region, achieving an imaging depth of up to ~900 µm.
Simultaneous Dual-region Functional Imaging in Miniaturized Two-photon Microscopy
Authors: Zhang Zixiao , Liu Shing-Jiuan , Mattison Ben , Yang Weijian
Published: June 2024

We demonstrate simultaneous dual-region in-vivo imaging of brain activity in mouse cortex through a miniaturized spatial-multiplexed two-photon microscope platform, which doubles the imaging speed. Neuronal signals from the two regions are computationally demixed and extracted.
Micro-camera Arrays for Ultra-widefield, Multi-site Cellular Resolution Calcium Imaging Across the Dorsal Cortex of Behaving Mice.
Authors: Cherkkil Arun , Hu Jia , Oladepo Ibrahim , Fausner Skylar , Surinach Daniel , Peters Ryan , Saxena Kapil , Horstmeyer Roarke , Kodandaramaiah Suhasa B.
Published: June 2024

Here, we introduce a miniaturized multi camera microscope that can simultaneously capture cellular resolution neural activity from multiple large fields of view of the cortex in a freely moving mouse.
Spatiotemporal Properties of Stimulus-evoked Responses in Mice Following Single Whisker Stimulation
Authors: Schloss Kaelyn H. , Wang Xiaodan , Padawer-Curry Jonah A. , Bice Annie R. , Bauer Adam Q.
Published: June 2024

We used novel peripheral stimulation methods to achieve highly precise single whisker targeting to examine spatiotemporal relationships between stimulus-evoked neuronal, metabolic, and hemodynamic activity in awake mice using wide-field optical imaging.
Dynamic Connectivity of Mouse Cortex Observed with Wide Field Optical Imaging
Authors: Bian Yuyang , Meyer-Baese Lisa , Wang Yunmiao , Jaeger Dieter , Keilholz Shella
Published: June 2024

Widefield optical imaging allows for novel methods of network-level functional connectivity analysis of neuronal voltage and hemodynamic signals to aid the interpretation of metabolic activation across mouse cortex.
An open-top light-sheet (OTLS) microscope for surveying gene expression in thick expanded tissues
Authors: Brenes David , Han Qinghua , Bishop Kevin , Glaser Adam , Liu Jonathan T. C.
Published: June 2024

We designed a novel non-orthogonal dual-objective open-top light-sheet microscope to resolve individual genomic/transcriptomic targets in thick hydrogel-based expanded fluorescence in situ hybridization labeled tissues at moderate tissue expansions.
Cerebral Autoregulation Analysis using Diffuse Correlation Spectroscopy on Adults undergoing Extracorporeal Membrane Oxygenation Therapy
Authors: Dar Irfaan A. , Khan Imad R. , Maddox Ross K. , Marinescu Mark A. , Busch David R. , Choe Regine
Published: June 2024

Here, we use DCS and MAP to analyze wavelet coherence and Pearson correlation coefficient in ECMO patients to evaluate neurological injuries. Results show neurologically injured patients had higher values compared to non-injured patients.
Speckle visibility spectroscopy laser-device for cerebral blood flow measurement
Authors: Mahler Simon , Huang Yu Xi , Dickson Maya , Yang Changhuei
Published: June 2024

Non-invasive measurement of cerebral blood flow presents a persistent challenge. This study endeavors to introduce a compact device for non-invasive cerebral blood flow measurements via speckle visibility spectroscopy device with remarkable sensitivity and temporal resolution.
Evaluating functional connectivity during motor imitation using diffuse optical tomography
Authors: Park Sung Min , George Tessa G. , Sobolewski Chloe M. , McMorrow Sophia R. , Yang Dalin , Nebel Mary B. , Tunçgenç Bahar , Vidal René , Marrus Natasha , Mostofsky Stewart H. , Eggebrecht Adam T.
Published: June 2024

High-density diffuse optical tomography assessed brain activity in neurotypical individuals during complex motor imitation. Functional connectivity derived from independent component analysis with reference varied between motor observation and imitation, correlating with behavior and imitation fidelity.
Multi-faceted approach to naturalistic functional neuromonitoring through a wearable, easy-to-setup, and 3-D aware modular fNIRS system
Authors: Xu Edward , Vanegas Morris , McCann Ashlyn , Mireles Miguel , Nguyen Michael , Meier Erin L. , Stefanik Joshua , Fang Qianqian
Published: June 2024

We present an ultra-lightweight wearable fNIRS system equipped with a suite of features, such as bendable modules, 3-D optode tracking, anatomically-derived head-caps, and real-time mounting guidance, specifically tailored towards simplifying measurements in naturalistic settings.
Mapping Brain Function Underlying Coherent and Scrambled Biological Motion Processing in Autistic School-Age Children Using HD-DOT
Authors: Yang Dalin , Svoboda Alexandra M. , George Tessa G. , Wheelock Muriah , Schroeder Mariel L. , Rafferty Sean , Sherafati Arefeh , Culver Joseph P. , Eggebrecht Adam T.
Published: June 2024

We used HD-DOT to image brain function in ninety-five school-age children with and without autism as they performed a passive biological motion perception task. Results show HD-DOT is sensitive to autism-associated differences in brain function.
Test-Retest Reliability of a Time-Domain Functional Near-Infrared Spectroscopy System
Authors: Dubois Julien , Field Ryan M. , Jawhar Sami , Koch Erin M. , Aghajan Zahra M. , Miller Naomi , Perdue Katherine L. , Taylor Moriah
Published: June 2024

We validate that Kernel Flow2 TD-fNIRS system is capable of measuring robust neural activity, during rest and sensory tasks, that is reliable across time and devices; thus enabling applications that require neuroimaging at scale.
Nonlinear to geometric focusing transition: beam self-cleaning and self-focusing critical power
Authors: Pushkarev D. V. , Rizaev G. E. , Seleznev L. V.
Published: June 2024

We propose an easy but effective approach to find a transition numerical aperture between the regimes of laser pulse filamentation with nonlinear focusing and with geometric focusing predominance. The suggested method based on the beam profile measurements allows correction of the data provided by spectra measurements. Using a simple semi-analytical model, we study the dependence of the transition numerical aperture on the pulse power and medium nonlinearity. The analysis shows that in condensed media the transition from the nonlinear to geometric focusing regime occurs at much tighter focusing than in air. Moreover, if the medium nonlinear refractive index is high enough, only the nonlinear focusing regime is observed even at numerical apertures close to one, which allows symmetric plasma channel formation.
A compact and low-cost solution for rapid frequency domain lifetime imaging
Authors: Singh Lakhvir , Hu Linghao , Walsh Alex J.
Published: June 2024

A cost friendly and compact frequency domain fluorescent lifetime microscope (FD-FLIM), with the potential of capturing lifetimes with resolution of a few nanoseconds, has been theorized and designed using detection signal processing to encode the phase shift of fluorescence emission within the intensity captured via camera. Model simulations of the system parameters illustrate the calibration process and effectiveness of the technique. The results will facilitate the development of a novel FD-FLIM that uses simple optical components and a conventional camera for improved modularity, ergonomics, software-interface, precision control, automation, compactness, and cost-effectiveness over traditional lifetime microscopes.
Automatic detection of prostate cancer via 3D microscopy and deep learning
Authors: Serafin Robert B. , Wang Rui , Chow Sarah , Bishop Kevin , Baraznenok Elena , Lan Lydia , True Lawrence D. , Liu Jonathan T. C.
Published: June 2024

We present an annotation free deep-learning-assisted segmentation pipeline to automatically identify healthy and malignant glands in 3D microscopy images of prostate biopsies stained with fluorescent analogs of H&E.
Deep-learning triage of 3D pathology data for improved disease detection while reducing pathologist workloads
Authors: Gao Gan , Wang Fiona , Brenes David , Song Andrew H. , Chow Sarah S.L. , Mahmood Faisal , Liu Jonathan T.C.
Published: June 2024

3D pathology can potentially improve disease detection, but the datasets are too large to review. We’re developing a deep-learning-based triage method to identify the highest-risk 2D sections within 3D pathology datasets for rapid pathologist review.
A multiscale multimodal imaging platform for high-throughput morphodynamics and spatial biology
Authors: Ma Hongqiang , Chen Maomao , Xu Jianquan , Liu Yang
Published: June 2024

Spatiotemporal biology is essential in biomedical research, linking large-scale spatial data, microenvironment, dynamic cell behaviors, and their underlying molecular characteristics. Our platform integrates dynamic quantitative phase imaging with hyper-plex fluorescence microscopy, enabling multiscale insights from mesoscale to nanoscale.
Miniature Objective Lens for Scattering-based Light Sheet Microscopy
Authors: Zhao Jingwei , Kim Yongjun , Sugimura Momoka , Marcelino Kenneth , Romero Rafael , Liang Brooke , Khan Michelle , Yang Eric , Kang Dongkyun
Published: June 2024

We have designed and built a miniature objective lens for scattering-based light sheet microscopy (sLSM). The objective lens achieved a high resolution, large field of view, and small field curvature.
Open-top Light-sheet Microscopy of Densely Labeled Clinical Specimens Enabled by Axially Swept Illumination
Authors: Bishop Kevin W. , Erion Barner Lindsey A. , Baraznenok Elena , Lan Lydia , Poudel Chetan , Brenes David , Serafin Robert B. , Vaughan Joshua C. , Glaser Adam K. , Liu Jonathan T.C.
Published: June 2024

High-quality optical sectioning is required for volumetric imaging of densely labeled clinical tissues. We describe an optimized open-top light-sheet (OTLS) microscope with axially swept illumination to provide improved optical sectioning over large fields of view.
Comparison of De-Noising Methods Applied to Intravital Imaging
Authors: Shukla Suryansh , Jung Yookyung , Entenberg David
Published: June 2024

Intravital imaging (IVI), used for revealing real-time dynamics of biological systems, often suffers from noise. Here, we, for the first time, compare various denoising methods to determine the best algorithms for IVI in several tissues.
Optimization and Application of a Pathology Computer Assisted Microscope (PathCAM) for Real-Time Slide Digitization and Analysis
Authors: Cooper Max S. , Ashman Kimberly , Maira Cooper , Halat Shams , Sholl Andrew , Wenk Carola , Fox Sharon , Summa Brian , Brown J. Quincy
Published: June 2024

We describe the development and optimization of PathCAM, a system for real-time slide digitization during clinical glass slide review, and initial real-time “expert-in-the-loop” applications including assessment of completeness of review, and annotation, segmentation, and computation.
Rapid Histology of Fresh Tissue Samples Using a Clinical-Compatible Stimulated Raman Imaging Device
Authors: Brinkmann Maximilian , Rezaei Maryam , Droop Ramon , Engwer Christoph , Neumann Felix , Lüpken Niklas , Dobner Sven , Hellwig Tim
Published: June 2024

We present a fully integrated, clinical-compatible SRS imaging device giving access to the complete Raman spectrum for histological tissue examination during tumor surgeries.
Effects of Laser Treatment on Cartilage Diffusion Properties
Authors: Iorizzo Tyler W. , Wright Santana , Childs James , Yaroslavsky Ilya , Altshuler Gregory , Yaroslavsky Anna N.
Published: June 2024

Nuclear Magnetic Resonance and optical imaging experiments were used to compare diffusion through cartilage samples before and after laser treatment.
Developing Methods for Characterizing Nuclear Signatures of Colon Polyps via Spectroscopic Optical Coherence Tomography
Authors: O’Kane Erin C. , Wang Wan , Highland Trey , Miller David A. , Wax Adam
Published: June 2024

We propose the use of spectroscopic OCT to characterize nuclear signatures of colon polyps ex vivo for early colorectal cancer detection. We justify using SOCT by accurately measuring the size of 4.78 µm polystyrene beads.
Tailored noise-suppressed STOC-T system for in vivo cellular-level imaging of the human retina
Authors: Mikuła-Zdańkowska Marta , Borycki Dawid , Węgrzyn Piotr , Wojtkowski Maciej
Published: June 2024

We introduce a modified experimental STOC-T system with an increased lateral resolution of ~3 µm and a high-speed phase modulator that optimizes illumination. The integration of this rapid phase modulator with a numerical averaging process facilitates a noticeable improvement in the signal-to-noise ratio (SNR). This enhancement's effects have been showcased through volumetric imaging of both a scattering object and the human retina.
Computational aberration correction enables full-thickness, neuronal-level human retinal imaging with spatio-temporal optical coherence tomography (STOC-T)
Authors: Borycki Dawid , Mikuła-Zdańkowska Marta , Węgrzyn Piotr , Wojtkowski Maciej
Published: June 2024

We demonstrate that spatio-temporal optical coherence tomography (STOC-T) supported by computational aberration correction provides neuronal-level imaging of the human retina. We show en face images depicting the amacrine cells, the multipolar retinal neurons.
In Vivo Evaluation of Hydrogel-Mediated Wound Healing Using Optical Coherence Tomography
Authors: Shah Shreyas , Song Jiyeon , Samanta Bibek R. , Sarkar Sohini , Crouch Michael S. , Eggleston Michael S. , Gerecht Sharon
Published: June 2024

We demonstrate in vivo monitoring of wound healing dynamics in mice treated with hydrogels of varying stiffness using OCT. Distinct cellular responses were visualized and tracked, revealing the potential of OCT for optimizing hydrogel-based treatments.
Quantitative Analysis of Cellular Dynamics: Unveiling the Impact of Varying Oxygen Saturations through High-Speed Optical Interferometric Imaging
Authors: Park Soongho , Nguyen Thine , Mutersbaugh John , Gandjbakhche Amir H.
Published: June 2024

Utilizing dynamic full-field optical coherence microscopy, we analyzed intracellular movement in aerobic cells with varying oxygen saturations. Employing a deep learning algorithm, we classified dynamic activities based on oxygen saturation, revealing insightful trends.
Utilizing optical coherence angiography to assess the acute and persistent effects of prenatal alcohol exposure on fetal brain vasculature in utero
Authors: Gutierrez Jessica , Singh Manmohan , Miranda Rajesh C. , Larin Kirill V.
Published: June 2024

Prenatal alcohol exposure diminishes cerebrovascular blood flow, impairing development. We analyze the acute and persistent effects of different alcohol dosages (1.5g/kg, 3g/kg, and 4.5g/kg) on fetal brain vasculature by utilizing correlation mapping optical coherence angiography.
Noninvasive Evaluation of Mouse Embryo Development Using Time-lapse Optical Coherence Microscopy
Authors: Wang Fei , Hao Senyue , Park Kibeom , Ahmady Ali , Zhou Chao
Published: June 2024

Optical coherence microscopy (OCM) offers volumetric imaging for non-invasive evaluation of embryo quality in in-vitro fertilization (IVF). Time-lapse OCM images of cryopreserved mouse embryos revealed development milestones, blastocyst grading, and stage timing inside the incubator.
Tracking the effect of PFOA exposure on mammary epithelial organoids longitudinally by OCT-structural functional imaging (OCT-SFI)
Authors: Yang Lin , Ji Pan , Miranda Buzetta Abel A. , Lockett Matthew R. , Oldenburg Amy L.
Published: June 2024

Utilizing OCT-SFI, this study uncovers substantial morphological alterations and inhibition of intracellular motility in mammary epithelial organoids exposed to PFOA over 12 days, introducing a novel long-term research tool to prioritize PFAS for carcinogenic potential.
Classification of Reconstructed Breast Optical Coherence Tomography Volumes via Compressed Sensing
Authors: Joasil Arielle S. , Song Cho Diego M. , Jerome Manuel J. , Hibshoosh Hanina , Hendon Christine P.
Published: June 2024

Identifying cancerous lesions in reconstructed optical coherence volumes using com-pressed sensing is imperative to deploying OCT to the clinical breast pathology workflow. We find that classification does not vary greatly with the sampling rate.
Prediction model for laser marking colors based on color mixing
Authors: Xue Zhishuang , Wan Xiaoxia , Wang Xueqiang , Qin Xiao , Song Kun
Published: June 2024

The current techniques for coloring surfaces using lasers necessitate the identification of numerous laser marking parameters, which is a laborious process. Furthermore, the quantitative analysis of generating a wide variety of colors through fewer sets of laser marking parameters is a huge challenge. This work employs a nanosecond laser to generate mixed structural colors from micro-nano structures on the surface of stainless steel in order to address these issues. Additionally, the color mixing principle is investigated in relation to these micro-nano structures. On this basis, the spectral reflectance of the primary color is mapped to the linear mixed color space, and the linear mixed color space is constructed by minimizing the linear deviation function. In this space, a precise mathematical model for color prediction is developed, which effectively captures the correlation between the primary color and the resulting mixed color. Four primary colors are created using four sets of laser marking parameters. Mixing these primary colors in varying proportions can achieve more than 100 new tones with rich colors. The average color difference ΔE
a
b
∗ and ΔE00∗ are 1.98 and 1.80, respectively. By utilizing this model to adjust the proportion of primary colors in each subgraph, an image with vibrant and rich colors is generated, thereby achieving the implementation of a structural color image based on mixed colors.
Growth and characterization of micro-LED based on GaN substrate
Authors: Wang Guobin , Huang Jinpeng , Wang Yang , Tao Tao , Zhu Xiaohui , Wang Ziwei , Li Kai , Wang Yuning , Su Xujun , Wang Jianfeng , Liu Bin , Cao Bing , Xu Ke
Published: June 2024

As the diminution of micro-LED pixels advances, the pivotal role of dislocation phenomena becomes increasingly pronounced. This study provides insight into the key characteristics and dominant mechanisms of GaN-based micro-LEDs by comparing the homoepitaxial and heteroepitaxial configurations. Our findings reveal that variability in V-shaped pits distribution markedly influences the performance and uniformity of micro-LED chips. While the homoepitaxial micro-LEDs, alongside significantly reduced dislocation density and residual stress, effectively preclude the formation of them and thus ensuring superior uniformity both within and among micro-LED chips. Notably, the external quantum efficiency (EQE) peak of homoepitaxial micro-LEDs surpasses that of heteroepitaxial variants by 40%. Motivated by the realization that the reduced MQW thickness at the sidewalls of V-shaped pit aids carrier injection, a great enhancement in EQE from 7.9% to 14.8% (@ 10 A/cm2) was achieved by the optimization of homoepitaxial structure. Therefore, the growth of micro-LED with lower dislocation density, lower residual stress, and epi-structure of low-energy-barrier MQWs demonstrated the profound impact on advancing micro-LED technology to obtain the performance of high uniformity, high brightness, and low power consumption.
Design of boadband THz multi-beam splitting metasurface
Authors: Teng Yan , Wang Tengyu , Li Chun , Huang Zhengwei , Jiang Ling
Published: June 2024

Generating multiple local oscillator (LO) beams by beam splitters is a crucial aspect of large heterodyne array receivers operating at terahertz (THz) frequencies, with over 100 pixels. Metasurfaces have received considerable attention due to their unique and flexible wavefront modulation capabilities. Nevertheless, the design of beam-splitting metasurfaces faces significant challenges in increasing the number of diffraction beams, improving power efficiency, and achieving greater homogeneity. A SA-GS-based design model for beam-splitting metasurfaces is proposed to achieve multi-beam with high power efficiency and homogeneity. As a proof of concept, we have designed and optimized a 16-beam splitting metasurface from 0.82 THz to 1.6 THz. The objective is to develop large-pixel THz multi-beam heterodyne array receivers and optical systems. The number of beams is also extended to 100-, 144-, 225-, and 289-beam configurations, with power efficiencies of 93.55%, 93.92%, 96.01%, and 96.18% at 0.85 THz, respectively. Moreover, the main beams exhibit excellent homogeneity. This model can be employed in the design of multi-beam metasurfaces with variable deflection angles and intensity ratios. Finally, the multi-beam splitting metasurface is fabricated, and the experimental measurement agrees with the simulation. This work presents an effective approach for the inverse design of beam splitters or holographic imaging devices.
Suspended Nanophotonic Waveguide for Isotope-Specific CO₂ Detection
Authors: Jágerská Jana , Salaj Jehona , Vlk Marek , Zakoldaev Roman , Seton Ragnar , Ctyroky Jiri , Alberti Sebastian , Aksnes Astrid
Published: June 2024

Spectroscopic detection of gases and their stable isotopes holds significant value in bio-sciences and climate studies. However, achieving high precision has long been confined to bulky and costly equipment. In this work, we introduce a nanophotonic waveguide that is capable of detecting CO₂ gas down to 20 parts per billion, and for the first time perform accurate stable isotope ratio measurements. The waveguide leverages a suspended membrane design with microstructured cladding, providing a 102% high evanescent field confinement factor, moderate loss of 3.4 dB/cm, and effective suppression of etalons. The δ¹³C isotope ratio precision of 0.2‰ was achieved, replicating the performance of high-end laser absorption spectrometers on a photonic chip. This marks the inaugural instance of on-chip, isotope-specific gas detection facilitating a compact and cost-efficient system scalable to sensor networks.
Temporal Point Spread Function Deconvolution in Time-resolved Fluorescence Lifetime Imaging using Deep Learning Model
Authors: Pandey Vikas , Erbas Ismail , Michalet Xavier , Ulku Arin , Bruschini Claudio , Charbon Edoardo , Intes Xavier
Published: June 2024

We report a deep RNN model for temporal point spread function (TPSFs) deconvolution in multimodal imaging systems to extract true sample fluorescence decay rate. It’s performance has been validated in phantom and well-plate data.
In Vivo Labeling and Detection of Circulating Tumor Cells with Fluorescent Molecular Contrast Agents
Authors: Pace Joshua , Ma Junxiong , Lee Jane , Srinivasarao Madduri , Kallepu Shivakrishna , Wang Lei , Hubbell Grace , Malankar Gauri , Whalen Riley , Wong Melissa H. , Gibbs Summer L. , Low Phil S. , Niedre Mark
Published: June 2024

The goal of this research is to develop a method to label, detect, and count circulating tumor cells in vivo with different types of injectable receptor targeted fluorescent molecular probes and near infrared (NIR) light.
Noninvasive cerebral blood flow sensing in humans in vivo using spatial correlations in parallel near-infrared spectroscopy
Authors: Nowacka Klaudia , Samaei Saeed , Dąbrowski Michał , Borycki Dawid
Published: June 2024

Optical methods can provide noninvasive approach for continuous cerebral blood flow (CBF) monitoring in humans in vivo. Diffuse correlation spectroscopy (DCS) is an established modality for qualitative CBF monitoring. DCS decodes the CBF from an analysis of the temporal correlations of the light scattered by the tissue. This, however, requires ultra-fast, generating vast amount of data to be processed. Instead of rapidly sensing temporal correlations, we can decode sample dynamics by quantifying speckle contrast, which is inversely proportional to the blood flow. Here, we analyze such an approach in the continuous-wave parallel interferometric near-infrared spectroscopy (CW-πNIRS).
Effect of neural source depth on diffuse optical tomography enhanced electroencephalography reconstruction
Authors: Qin Yutian , Wu Jingyi , Bulger Eli , Cao Jiaming , Dehghani Hamid , Shinn-Cunningham Barbara , Kainerstorfer Jana M
Published: June 2024

DOT-enhanced EEG localization has high spatiotemporal resolution but the resolution decreases for deep neural sources. We analyzed the reconstruction of neural sources at different depths.
MCX-LLM: an experiment in bridging natural language problem descriptions with quantitative scientific simulations
Authors: Yen Fan-Yu , Fang Qianqian
Published: June 2024

We explored large language models (LLM) in converting natural language descriptions into machine-readable inputs to run Monte Carlo simulations. Albeit limited success, LLM shows the potential to simplify scientific software interfaces.
Speckle-Plethysmography Waveform Analysis Predicts Continuous Hemodynamic Changes
Authors: Parker Helen E. , Mostashari Golnar , Quang Timothy , Hill Brian , Bowling Andrea , Perreault John , Kanthi Yogendra , Tromberg Bruce J.
Published: June 2024

Wearable optical sensors can be used for non-invasive hemodynamic monitoring. Speckle-plethysmography (SPG) measures blood flow. We report on the use of SPG for tracking changes in hemodynamic state in an adult healthy population.
Development of speckle contrast line scanner for estimation of coagulation depth in burn wounds
Authors: Johansson Johannes D. , Hultman Martin , Saager Rolf B.
Published: June 2024

A novel system and method for 3D laser speckle imaging of coagulation depth in partial thickness burn wounds using a line laser to provide depth-varying imaging is presented.
A Comparative Study of Blood Flow Monitoring Using Interferometric Diffusing Wave Spectroscopy, Speckle Contrast Optical Spectroscopy, and Diffuse Correlation Spectroscopy
Authors: Majeski J.B. , Forti R.M. , Chong S.H. , Ramachandran N.V. , Abramson K. , Aparanji S. , Zhao M. , Srinivasan V.J. , Baker W.B. , Yodh A.G.
Published: June 2024

We compared blood flow index measurements and their coefficients of variation obtained with three diffuse optical blood flow monitoring techniques on intralipid phantoms and on the forearm during an arm-cuff ischemia protocol.
A Real-Time FPGA-Based DCS System for Blood Flow Monitoring
Authors: Moore Christopher H. , Sunar Ulas , Lin Wei
Published: June 2024

A field-programmable gate array (FPGA) design was created to perform all diffuse correlation spectroscopy (DCS) computations necessary to derive a blood flow index on a single chip. A good match was observed between cuff ischemia results from our FPGA system and a reference standard DCS system.
Novel frequency-domain data types for deeper sensitivity to tissues
Authors: Sassaroli Angelo , Tavakoli Fatemeh , Blaney Giles , Frias Jodee , Fantini Sergio
Published: June 2024

We carry out a simulation study for the application of novel data types in frequency-domain near-infrared spectroscopy during arterial or venous occlusion of the human forearm.
Enhanced, Non-Invasive, Blood Flow Measurements Enabled by Coherence Gating
Authors: Robinson Mitchell B , Renna Marco , Franceschini Maria Angela , Carp Stefan A
Published: June 2024

Diffuse correlation spectroscopy enables non-invasive measurements of blood flow. Recent advances have enhanced measurement capabilities using interferometric detection. In this work, we report our progress on the development of pathlength selective, interferometric diffuse correlation spectroscopy.
Assessing Oxygenated Hemoglobin Concentration by Time-Domain Diffuse Optical Spectroscopy at 1064 nm
Authors: Di Sieno L. , Bossi A. , Sangalli F. , Torricelli A. , Durduran T. , Tachtsidis I. , Pifferi A. , Mora A. Dalla
Published: June 2024

The assessment of concentration of oxygenated hemoglobin is a crucial parameter for oximetry application. We explore the possibility of using 1064 nm wavelength to assess the concentration of oxygenated, discussing possible advantages and issues.
Fast acquisitions of heartbeat-induced absorption changes with compact time-domain diffuse optics system
Authors: Avanzi E. , Talala T. , Mora A. Dalla , Nissinen I. , Nissinen J. , Di Sieno L.
Published: June 2024

This work describes a compact and potentially wearable health monitoring time-domain diffuse optics system. It employs a CMOS driver, a laser diode and a 256-channel SPAD line sensor and detects heartbeat induced absorption changes.
Wearable Broad Bandwidth Frequency-Domain Near Infrared Spectroscopy (fd-NIRS)
Authors: Lahade Shashikant , Yazdi Siavash , Green Michael M. , O’Sullivan Thomas D.
Published: June 2024

We present a miniature dual-wavelength fd-NIRS device for wearable real-time physiological monitoring. Based on a custom CMOS circuit, wearable device demonstrates accurate phase and amplitude measurements for modulation frequencies up to 300 MHz.
Effects of Matrix Conditioning strategies on Multifrequency High-density Diffuse Optical Tomography
Authors: Zhang Chengfeng (Aaron) , Fan Weihao , Eggebrecht Adam T.
Published: June 2024

High-frequency modulation has been shown to provide superior image quality over continuous wave high density diffuse optical tomography (HD-DOT). Herein, we evaluated matrix conditioning strategies for multi-frequency HD-DOT for potential improvements in imaging performance.
Experimental validation of multi-parameter diffuse optical tomography using deep-learning
Authors: Dale Robin , Zheng Biao , O’Sullivan Thomas , Dehghani Hamid
Published: June 2024

This study presents experimental validation of a deep-learning (DL) model for simultaneous 3D reconstruction of absolute absorption and reduced scattering. The model improved structural similarity and contrast, but increased cross-talk compared to an analytical algorithm.
Comprehensive dataset of absorption and scattering spectra of in-vivo biological tissues using time-domain diffuse optical spectroscopy
Authors: Bargigia Ilaria , Karremans Siënna , Damagatla Vamshi , Bossi Alessandro , Taroni Paola , Pifferi Antonio
Published: June 2024

Through time-domain diffuse optical spectroscopy measurements, we present a comprehensive overview of the optical properties (reduced scattering and absorption co-efficients) found in in-vivo tissues. Furthermore, this dataset represents a working example of Open Data.
Diffuse Optical Spectroscopy of Lactating and Non-lactating Mammary Physiology
Authors: Boamfa Ana , Coverstone Caitlin , Abdalsalam Ola , de Almeida Barreto Ana Flavia , Wei Alicia , de Wolf Johanna R. , Schoustra Sjoukje M. , O’Sullivan Thomas D. , Bosschaart Nienke
Published: June 2024

We apply broadband Diffuse Optical Spectroscopy (DOS) to investigate optical and physiological differences between i) lactating and non-lactating breasts, ii) the areolar and non-areolar breast region, and iii) lactating breasts prior and post milk extraction.
Hemodynamic Monitoring of Bone Healing with Diffuse Correlation Tomography and Spatial Frequency Domain Imaging
Authors: Choe Regine , Lee Youngjoo , Majeski Joseph B. , March Alyson , Li Zihao , Dar Irfaan A. , Ren Jingxuan , Han Songfeng
Published: June 2024

The effects of age and parathyroid hormone treatment on bone healing were investigated using non-invasive longitudinal diffuse correlation tomography and spatial frequency domain imaging of bone injury in murine femurs.
New algorithm strategies in structural and functional photoacoustic computational tomography
Authors: Gao Rongkang , Liu Chengbo
Published: June 2024

This work presents new tools for high dynamic, high-resolution, and deep biological 3D structural and functional imaging research and applications.
Exploring Subsurface Raman Spectroscopy for Non-invasive Sex Determination of Chicken Eggs
Authors: van den Tweel Lennard , Ariese Freek , van der Pol Carla , van den Brand Henry
Published: June 2024

Raman spectroscopy of extra -embryonic blood vessels confirmed sex-specific differences in hemoglobin levels and near-infrared fluorescence intensity. These insights incited ongoing development of non-invasive methods for in-ovo sex determination of chicken eggs using diffuse spectroscopy techniques.
Detection of structural changes in DNA due to methylation using Raman spectroscopy
Authors: Kanae Haruki , Ito Terumasa , Tsukakoshi Kaori , Inaba Shintaro , Tomizawa Mizuki , Das Kaustav , Ikebukuro Kazunori , Misawa Kazuhiko
Published: June 2024

Steric conformational changes in the guanine quadruplex (G4) structure due to DNA methylation are associated with cancer. We show that Raman spectroscopy can be used to detect the conformational changes in the G4 structure.
Creating tunable complex light-emitting optical phantoms utilizing multifilament mixing based 3-D printing
Authors: Ragunathan Rahul , Simmons Victoria , Mireles Miguel , Lewis Aiden , Fang Qianqian
Published: June 2024

We report initial results for direct 3-D printing of heterogeneous optical phantoms with tunable light emitting levels using a multi-color mixing filament extruder. We report preliminary characterizations of the emitted light temporal and spatial profiles.
Manipulating exciton confinement for stable and efficient flexible quantum dot light-emitting diodes
Authors: Hu Xiaoyun , Yang Jianfang , Tu Yufei , Su Zhen , Zhu Fei , Guan Qingqing , Ma Zhiwei
Published: June 2024

Flexible quantum dot light-emitting diodes (QLEDs) show great promise for the next generation of flexible, wearable, and artificial intelligence display applications. However, the performance of flexible QLEDs still lags behind that of rigid substrate devices, hindering their commercialization for display applications. Here we report the superior performance of flexible QLEDs based on efficient red ZnCdSe/ZnS/ZnSe QDs (A-QDs) with anti-type-I nanostructures. We reveal that using ZnS as an intermediate shell can effectively confine the exciton wavefunction to the inner core, reducing the surface sensitivity of the QDs and maintaining its excellent emission properties. These flexible QLEDs exhibit a peak external quantum efficiency of 23.0% and a long lifetime of 63,050 h, respectively. The anti-type-I nanostructure of A-QDs in the device simultaneously suppresses defect-induced nonradiative recombination and balances carrier injection, achieving the most excellent performance of flexible QLEDs ever reported. This study provides new insights into achieving superior performance in flexible QD-based electroluminescent devices.
Advancements in Microscopy Image Analysis: A MATLAB-Based Histogram Segmentation Approach for Particle Detection
Authors: Esmaeili Shahriar
Published: June 2024

This paper presents a MATLAB-based method for particle detection in microscopy images using histogram segmentation. It enhances accuracy in identifying particle clusters, offering significant applications in biomedical imaging and material science research.
Characterization of functional groups of Olive Leaves adopting Fourier transform Infrared spectroscopy
Authors: Khedr Amal Abdlelfattah
Published: June 2024

Vibrational molecular spectroscopic technique known as Fourier transform infrared spectroscopy (FTIRS) has been utilized for detection of active function groups in two different varieties of olive leaves. Olive leaves have been collected from two farms lie in Al Jouf city in the north of the Kingdom of Saudi Arabia. Phenolic compounds which are essential in various functional food and folk medicine were investigated.
A novel hyperspectral imaging tool for preclinical and translational investigations of brain tissue physiology and pathology
Authors: Giannoni Luca , Toaha Anam , Marradi Marta , Bonaudo Camilla , Puppa Alessandro Della , Pavone Francesco S.
Published: June 2024

We present a novel hyperspectral imaging setup enabling high-resolution, fast reconstruction of large spectral datasets of brain tissue reflectance and fluorescence, with flexibility in the wavelengths selection, for in vivo and ex vivo preclinical applications
Spiral-Shaped SPR-PCF biosensor with Circular Ring
Authors: Mittal Shweta , Saharia Ankur , Tiwari Manish
Published: June 2024

This work presented a Spiral-Shaped SPR-PCF biosensor with a Circular Ring. This work possesses the phase matching condition at 790 nm for the refractive index of analyte (n
a
) is 1.40 having a peak of confinement loss of 2.001553 dB/cm.
Deep brain monitoring for extremely low-birth-weight infants utilized time-domain diffuse optical spectroscopy in transmittance mode
Authors: Suzuki H. , Imanishi T. , Suzuki N. , Okuyama T. , Homma S. , Yoshimoto K. , Iida T. , Mimura T. , Wada H. , Ohmae E. , Shimizu M. , Ueda Y.
Published: June 2024

We developed a time-domain diffuse optical spectroscopy system for monitoring deep brain hemodynamics in premature infants in transmittance mode in the clinical setting and showed the system can measure the premature head in transmittance mode.
Deep Learning-based Automated Cell Viability Measurement in Tissue Scaffold using OCT
Authors: Shih Meijie , Wang Jiaying , Yu Xiaojun , Gan Yu
Published: June 2024

We proposed a deep learning-based approach to analyze cell viability from tissue scaffold images obtained from optical coherence tomography. Experimental results demonstrated the distinct viability patterns between active and dead cells and 3D visualization of cell distribution.
Auto-calibrated method recovers absolute optical properties of turbid media in a cuvette geometry
Authors: Blaney Giles , Sassaroli Angelo , Frias Jodee , Tavakoli Fatemeh , Fantini Sergio
Published: June 2024

Measuring scattering samples’ absolute optical properties (absorption and reduced scattering coefficients) is valuable in bio-medicine and beyond. This work presents experimental results recovering these properties in a standard cuvette volume with less than 3 % error.
Computational Model for Blood Pressure Estimation Using Pulse Wave Transit Time
Authors: Mizuno Haruka , Tabuchi Tohko , Nakabayashi Mikie , Ichinose Masashi , Ono Yumie
Published: June 2024

The squared value of pulse transit time data measured between two photoplethysmography sensors placed on the upper limb showed a potential for non-invasive, cuffless, and continuous blood pressure estimation.
Simulation of UV-visible Photoacoustic Imaging Using Metalenses
Authors: Barulin Aleksandr , Park Hyemi , Kim Inki , Park Byullee
Published: June 2024

High numerical aperture and micron-thick metal lenses solve the challenges of ultraviolet-visible photoacoustic microscopy, enabling simultaneous imaging of cellular DNA/RNA and blood vessels. Our simulation results show that these lenses hold promise for miniaturization for the advancement of portable photoacoustic microscopy.
Multidimensional Compressive Fluorescence Microscopy
Authors: Farina A. , Ghezzi A. , Lenz A.J.M. , Soldevila F. , Tajahuerce E. , Vurro V. , D’Andrea C.
Published: June 2024

A system for multispectral lifetime imaging based on single-pixel camera and compressed-sensing is presented together with two algorithms devoted to the improvement of the spatial resolution and the global reconstruction time.
In-process Monitoring of Bioreactors via Diffuse Optical Tomography with Genetically Encoded Fluorescent Tags
Authors: Gorecki J. , Cao J. , Redwood-Sawyyer C. , Lindeque R. , Polizzi K. , Kontoravdi C. , Dehghani H. , Rowlands C.
Published: June 2024

Here we propose to use fluorescent tags which are genetically modified to respond to various cell stimuli, such as lactate concentration, in combination with diffuse optical tomography to reconstruct 3D maps of in-situ cell behavior.
Label-free Imaging with Photonic Crystal Surface for Hematopoietic Stem Cell Differentiation
Authors: Zhuo Yue , Choi Ji Sun , Marin Thibault , Yu Hojeong , Harley Brendan A. , Cunningham Brian T.
Published: June 2024

With the Photonic Resonator Outcoupler Microscopy (PROM), it is possible to detect and monitor weak-adhesive HSC adhesion without labeling. These findings indicate that PROM can be used to quantitatively and dynamically study HSC adhesion.
High throughput evanescent-wave biosensor for the early-stage detection of biomarkers in liquid biopsies
Authors: Dallari Caterina , Perego Laura , Gardini Lucia , Falciani Chiara , Credi Caterina , Pavone Francesco Saverio
Published: June 2024

Innovative liquid biopsy technologies, exploiting total internal reflection spectroscopy, enable highly sensitive biomarkers detection. A compact, low-cost microscope exhibiting impressive detection limits and reproducibility was built, making it a promising diagnostic tool for real-time disease monitoring, applicable to human biofluids.
Non-invasive Cerebral Hemodynamic Monitoring in a Porcine Cardiac Arrest Model
Authors: Habash Shakeeb , Muldoon Ailis C. , Mazumder Dibbyan , Robinson Mitchell B. , Carr Bryce , Jung Ki-Tae , Koo Bonsung , Creed Ekaterina , Lee Kichang , Silverman Michael G. , Carp Stefan A.
Published: June 2024

Non-invasive optical monitoring holds potential for assessing cerebral hemodynamics post-cardiac arrest, as shown in a porcine cardiac arrest model, highlighting its potential to enhance patient care and outcomes.
Long-term monitoring of an aneurysmal subarachnoid hemorrhage patient using diffuse correlation spectroscopy
Authors: Muldoon Ailis , Robinson Mitchell B. , Habash Shakeeb , Yang Joanna , Sunwoo John , Gelman Justin , Webb Andrew , Rosenthal Eric , Franceschini Maria Angela , Chung David Y. , CARP Stefan A.
Published: June 2024

We present a case study for long-term DCS monitoring of an SAH patient. This case study demonstrates that DCS is suitable for continuous bedside monitoring and that MAP is a poor marker of cerebral perfusion.
FREEnet: a dynamic deep-learning model for freehand diffuse optical tomography
Authors: Dale Robin , O’Sullivan Thomas , Dehghani Hamid
Published: June 2024

A deep-learning (DL) model for handheld diffuse optical tomography is presented. The fully convolutional network can reconstruct 3D absorption and scattering from arbitrarily undersampled scan data at a rate of 18.5Hz, enabling real-time imaging.
Silicon Photonics MEMS Platform-based Optomechanical Ultrasound Sensor Array for 3D Photoacoustic Deep Brain Region Imaging
Authors: Nam Sangwoo , Choi Dongju , Han Sangyoon , Yu Jaesok
Published: June 2024

We propose a silicon photonic optomechanical ultrasound sensor array for 3D photoacoustic imaging of a mouse brain’s deep region. The performance evaluation and 3D photoacoustic in-vitro imaging were conducted for the feasibility study.
Quantifying Myelin Degradation Using Quantitative Birefringence Microscopy and Deep Learning
Authors: Gray Alexander J. , Robinson Rhiannon , Novoseltseva Anna , Li Shuying , Berghol Samer A. , Packard Logan , Moore Tara L. , Rosene Douglas L. , Bigio Irving J.
Published: June 2024

We report on an object detection network for automatically identifying structurally altered myelin in the brain tissue of a rhesus monkey model of limited cortical injury to be used for direct quantification of myelin damage.
Training Materials and Documentation Methods in NeuroDOT, a Toolbox for Optical Brain Mapping
Authors: Segel Ari , Speh Emma , Eggebrecht Adam
Published: June 2024

Multiple training methods are essential to facilitate global engagement and harmonization in optical brain mapping. We detail the methodology employed by NeuroDOT for creating documentation and trainings for use by the broader optical neuroimaging community.
An independent replicability study of MesoNet for automated segmentation and landmark identification in functional widefield optical imaging in mice
Authors: Yuan Aurora , Fisher Hayden B. , Padawer-Curry Jonah , Bauer Adam Q. , White Brian R.
Published: June 2024

MesoNet is an automated landmark identification and segmentation program for widefield optical imaging in mice. Using a multi-institutional dataset to assess external reliability we find promising results, but errors that prevent use without retraining.
Neurovascular Coupling during Cortical Slow Wave Activity in the Neocortex
Authors: Chetia Sumana , Casas-Torremocha Diana , Suarez-Perez Alejandro , Dragojevic Tanja , Sanchez-Vives Maria V. , Durduran Turgut
Published: June 2024

Slow wave activity is an intrinsic brain activity, and its investigation is relevant in both research and clinical domains. This work focusses on probing spatio-temporal aspects of neurovascular coupling during this cortical activity.
Monitoring of Brain Oxygenation with NIRS Under Lower Body Negative Pressure
Authors: Kacprza Michał , Sawosz Piotr , Krej Mariusz , Gerega Anna , Sudakou Aleh , Lipiński Kamil , Dziuda Łukasz , Wojtkiewicz Stanisław , Liebert Adam
Published: June 2024

The study investigated cerebral oxygenation in healthy volunteers using in-house developed NIRS, focusing on hemodynamic responses to position of a membrane of the in-house developed lower body negative pressure (LBNP) chamber.
Early Caries Enamel Detection through Dual-spectrometer Raman Polarized System
Authors: Chen Fang , Yang Shan
Published: June 2024

Investigating dental hard tissues, we designed a dual-spectrometer Raman polarized system with CCD and InGaAs detectors. This innovative tool assesses enamel properties, aiding early caries detection and linking them to dental issues.
Optical imaging of HIF-1α mediated metabolic changes in the radio-resistant head and neck squamous carcinoma cells
Authors: Yan Jing , Gonçalves Carlos Frederico Lima , Saha Pranto Soumik , Zhu Caigang
Published: June 2024

We demonstrate that an optical microscope can be a cost-effective tool for non-destructive characterization of HIF-1α induced metabolic reprogramming under RT stress in the acquisition of radio-resistance in HNSCC for therapeutic discovery.
Cerebral perfusion monitoring during acute stroke therapy with Diffuse Correlation Spectroscopy
Authors: Raghu Vishnukumar , Mohammad Parveen Penaz Sultana , Letavay Amy Arianna , Amin Sheyar Wala , Mokin Maxim , Parthasarathy Ashwin B
Published: June 2024

We report cerebral blood flow (CBF) measurements utilizing Diffuse Correlation Spectroscopy (DCS) carried out on patients undergoing revascularization therapy for acute ischemic strokes.
Functional Connectivity Changes in Mouse Models of Maple Syrup Urine Disease
Authors: Lavery Sarah , Adepoju Temilola , Fisher Hayden B. , Chan Claudia , Yuan Aurora , Kuhs Amanda , Ahrens-Nicklas Rebecca C. , White Brian R.
Published: June 2024

Using optical intrinsic signaling, we investigated resting state functional connectivity in mouse models of Maple Syrup Urine Disease before and after experimental metabolic crisis. We observed significant changes between control and experimental groups.
Characterization of Scattering for Whole Blood Oximetry in Sub-Diffuse Regimes
Authors: Elgabori Osama , Scammon William B. , Strong Kelly R. , Cook Keith , Kainerstorfer Jana M.
Published: June 2024

We propose a method for accounting for scattering in oximetry performed on whole blood. In this sub-diffuse regime, the diffusion approximation fails and we use Mie theory for characterization of scattering in whole blood.
Estimating the Optical Properties of Calf Brain during Thermal Treatment
Authors: Bossi Alessandro , Bianchi Leonardo , Saccomandi Paola , Pifferi Antonio
Published: June 2024

Thermal therapies are potentially less painful than surgery but lack accurate monitoring. We explore changes in the optical properties of calf brain during treatment, using time-domain diffuse optics and investigate their relation to damage.
Comparison of Resting-State Hemodynamic Parameters of the Vastus Lateralis Muscle Measured with TD NIRS in Middle-Aged and Old Population
Authors: Nabacino M. , Amendola C. , Pilotto A. , Ansaldo M. , Maggio M. , Salvi M. , Contini D. , Spinelli L. , Torricelli A. , Lauretani F. , Re R.
Published: June 2024

Absolute values of resting-state muscle hemodynamic parameters of 62 volunteers were measured using Time-Domain Near-Infrared Spectroscopy. Significant variations are observed with gender, likely related to adipose tissue thickness, while no differences are found with age.