bluuebunny/crossref_metadata_2025 huggingface dataset:
KHẢO SÁT KIẾN THỨC, THÁI ĐỘ, THỰC HÀNH VỀ PHÒNG CHỐNG DỊCH COVID-19 CỦA SINH VIÊN TRƯỜNG CAO ĐẲNG Y TẾ HÀ NỘI NĂM 2020
Authors: Tùng Bùi Huy , Nguyệt Minh Hà Thị , Thu Hường Lê Thị , Hồng Ngọc Nguyễn Thị , Huyền Nguyễn Thanh , Bảo Nghi Trần Đỗ
Published: July 2021

The research surveyed 434 nursing students at Hanoi Medical College for 2 months (October-November 2020). Synthesizing students’ scores on knowledge, attitude, and practice of COVID-19 epidemic prevention showed that 74.9% of students had good knowledge, and 25.1% which still didn’t achieved. The proportion of students with a good attitude was 93.2%, not good 6.8%. Research results showed that the proportion of students whose practices wasn’t good is quite high (56.3%), only 43.7% of students had good practices. The above situation of students shows that it is essential to improve and consolidate drasticmeasures to deter students from disease prevention.
STUDY ON THE PRODUCTION PROCESS OF SINGLE DOSE MEASLES-RUBELLA COMBINED VACCINE AT POLYVAC
Authors: Huong Nguyen Thuy , Hoa Le Thi , Hung Le Quoc , Huong Ngo Thu , Hien Nguyen Dang
Published: July 2021

Center for Research and Production of Vaccines and Biologicals (POLYVAC) has conducted “Study on the production process of single dose measles-rubella combined vaccine (MRVAC)” (1dose/vial) in lyophilized form to make it convenient to use for vaccination services and export. The results have determined  the formulation of MRVAC final bulk, freeze-dried process. The quality of researched products met WHO and Vietnam Pharmacopoeia V standards in both visual tests of lyophilized vaccine cake and after being reconstituted with sterile water for injection, the titer of measles virus ranges from 4.03 to 4.28 lgPFU/0.5mL and titer of rubella virus is from 3.79 to 3.98 lgPFU/0.5 mL. Results on thermal stability test when incubating the vaccine at 37oC/7days, the titer of measles virus decreased from 0.79 to 0.96 lgPFU/ 0.5mL and titer of rubella virus decreased from 0 to 0.18 lgPFU/0.5mL. Residual moisture content ranges from 0.35 to 0.72%. pH of vaccine is stable, ranges from 7.42 to 7.62.
SITUATION AND SOME RELATED FACTORS OF PHYSICAL ACTIVITIES AMONG ADUTLS IN PHAN DINH PHUNG WARD, THAI NGUYEN CITY IN 2019
Authors: Hien Nguyen Van , Tuan Nguyen Manh , Trang Le Thi Huyen
Published: July 2021

Objective: To describe the situation and some related factors of physical activity among adults inPhan Dinh Phung ward, Thai Nguyen city in 2019.Method: a cross-sectional descriptive studies was conducted on 495 adults in Phan Dinh Phungward, Thai Nguyen city. Data were collected by face-to-face interview using global physical activityquestionnaire (GPAQ).Results: The rate of high-level physical activity in men was 62.1% and for women was 47.8%, with43.8% of women and 62.1% of men achieving physical activity according to WHO recommendations.Age, location convenience, status of hypertension, diabetes, and cardiovascular disease weresignificant related factors of physical activity.
CHARACTERISTICS OF USING 3RD AND 4TH GENERATION CEPHALOSPORIN AT THONG NHAT HOSPITAL
Authors: Thai Hoang Duc , Minh Trinh Nhut , Chi Bui Dang Phuong
Published: July 2021

Objective: Describing characteristics of using 3rd and 4th generation Cephalosporin antibiotics at Thong Nhat Hospital in the period of 2019 - 2020.Subjects and methods: cross-sectional descriptive study on 158 medical records of patients discharged from hospital from February 20th, 2020 to February 24th, 2020 (5 days) was kept at themedical records store, Department of general planning, Thong Nhat hospital.Results: The majority of patients had no diagnosis of infection but had signs of infection, accounting for 69.92%. Duration of C3G/C4G antibiotic treatment of group with signs of infection was 9.22± 2.79 days. Cefotaxim was used the most, accounting for 26.58%. The majority of indications for the use of antibiotics C3G/C4G for patients were appropriate or partly appropriate, accounting for 74.05%. Efficiency of using appropriate or partially appropriate antibiotics had achieved high results, accounting for 39.24%. Most of the cases diagnosed or showed signs of infection after using antibiotics C3G/C4G obtained treatment efficacy, accounting for 75%. The majority of patients using C3G/C4G antibiotics achieved effectiveness in preventing wound infection in the patient with no signs of infection, accounting for 77.78%. The rate of elevated liver enzyme was 7.59%, the rate of thrombocytopenia was 3.16%.Conclusion: Duration of C3G/C4G antibiotic treatment of group with signs of infection was 9.22 ± 2.79 days. Cefotaxim were used the most. Most of indications to use antibiotics C3G/C4G were appropriate or partially appropriate. Efficiency of using appropriate or partially appropriate antibiotics had achieved high results. The rate of elevated liver enzyme was 7.59%, the rate of thrombocytopenia was 3.16%.
AWARENESS OF DRUG USE OF OUTPATIENTS AT THONG NHAT HOSPITAL
Authors: Hiep Bui Tung , Nhan Nguyen Huu , Phuoc Nguyen Huu , Huong Bui Dang Lan
Published: July 2021

Objective: To survey outpatient awareness of drug use at Thong Nhat Hospital.Subjects and methods: cross-sectional descriptive study on 120 patients who were given health insurance drugs waiting to take drugs, before entering the counseling room from September 1st, 2020 to December 1st, 2020 at the Thong Nhat hospital.Results: Patients based on reference of the drug name in the prescription at 51.15% and take drugs each time according to the prescription when being prescribed many drugs at the same time (57.50%). Using refresh water to take medicine accounted for 68.33%. There were 59.16% of patients taking the whole pill. Some patients took medicine following the other way such as breaking the pill before taking it (11.67%); Dissolve the drug in water (7.5%). Most of the patients (89.17%) learned about drug instructions. Doctors were the source of information for patients (61.67%) and the majority ofpatients (57.5%) heard counseling on drug use.Conclusion: The proportion of patients based on reference of the drug name in the prescription and taking each time according to the prescription when being prescribed many drugs at the sametime was moderate. Most patients used refresh water to take medicine and take whole pill. Most patients learned about medication instructions. Physicians were the primary source of informationfor patients and the majority of patients heard advice about drug use.
EXPECTATIONS OF ORAL HEALTH CARE SERVICES OF FIRST-YEAR MEDICAL STUDENTS AT HANOI MEDICAL UNIVERSITY 2020 - 2021
Authors: Thao Vu Thi Phuong , Tuan Vu Manh , Toan Ngo Van
Published: July 2021

Objective: To describe the expectations of oral health care services of first-year medical students at Hanoi Medical University.Subject and method: A cross-sectional descriptive study was applied with data collection by using online interview. Information of expectations about the elements of oral health care services wasrecorded and analysis according to the Likert scale. The study was carried out on 574 subjects. Results: Study participants had high expectations in the cleanliness of medical facilities, accountingfor 74.56%. Influencing factors of expectations in treatment procedures and treatments were informed about side effects, risks of treatment; were examined on time (mean expected score was 3.9; 3.87, respectively). Study participants have high expectations for all items in communication factors (mean score was above 3.92) and treatment outcomes (mean expected scores are over 3.89). The items with low expectations were the need to give a test/X-ray order, convenient and reassuring medical facility location (mean expected score of 3.45; 3.49 and 3.57 respectively).Conclusion: Most of the study subjects had high expectations about the elements of health care services. In which, the highest expectation belongs to the items of communication between doctor -patient and treatment results.
CHARACTERISTICS OF PATIENTS WITH BRAIN INJURY DUE TO LAND TRAFFIC ACCIDENTS TREATED AT NINH BINH PROVINCIAL GENERAL HOSPITAL IN 2018
Authors: Hien Luong Xuan , Tien Nguyen Quoc , Phuong Tran Thi
Published: July 2021

This was an epidemiological study through a retrospective review of 215 medical records of patients with brain injury due to land traffic accidents treated at Ninh Binh Provincial General Hospital. Theresults showed that the majority of research subjects were male, accounting for 80.5%; The age group with higher prevalence of accidents was under 30 years old, accounting for 31.2%; The agegroup of 50-59 years old was at lower prevalence of accidents accounting for 15.8%. The time of the higher chance of occurring accident was between 4p.m and 11p.m daily. The majorityof traffic accidents occurred when riding motorbikes and electric bicycles (82.3%). About 75.0% of the victims received first aid with a gauze bandage; 5.4% of them had broken bones fixed. Amongthe brain injuries, 34.4% of the victims suffered from frontal trauma; 29.3% had unilateral cerebral hemisphere injury, 24.2% had temporal region injury, 15.8% had occipital region injury, and 8.4% ofvictims had parietal injury. More than half of the study subjects (53.5%) were hospitalized for 7-10 days. The rate of patients who have been discharged from the hospital with improved symptoms was81.4%; and 14.9% of patients were transferred to higher level hospitals.
RESULTS OF TREATMENT OF CHILDREN WITH IDIOPATHIC NEPHROTIC SYNDROME AT BAC NINH OBSTETRIC AND CHILDREN'S HOSPITAL
Authors: Chi Vu Thi , Phuong Ta Thi Thanh , Kien Pham Trung
Published: July 2021

Objectives: describe the clinical characteristics, laboratory findings and evaluate the results of treatment of idiopathic nephrotic syndrome in pediatric patients at Bac Ninh Obstetrics and Children’sHospital. Method: descriptive study. From January 1, 2020 to June 30, 2021.Result: Fiffty two children were recruited into the study sample. Males got involed in this disease more than females with a ratio male to female of 2.05/1 (there were 17 women and 35 men). The meanage was 6,75 years (from 1 to 15 years old), 55,8% of them were under 5 years old. The percentages of severe edema was 7,7%; increased blood pressure was 9.6%; anuria was 19.2%. The average 24-hour proteinuria was 143.46 mg/kg/day; average protein/creatinine ratio was 878.08 mg/mmol. The percentages of patient with good treatment results is 76.9%. Proteinuria, protein/creatinine in urinedecreased significantly after treatment (p<0.05). Blood albumin increased and cholesterol decreased significantly after treatment (p<0.05).Conclusion: Children with idiopathic nephrotic syndrome most were males, young age, severe edema, severe reduction in blood protein and proteinuria. The disease was completely treatable and monitored locally
MỨC ĐỘ HÀI LÒNG CỦA NGƯỜI BỆNH ĐẾN KHÁM VÀ ĐIỀU TRỊ TẠI CÁC CƠ SỞ Y TẾ Ở VIỆT NAM. NGHIÊN CỨU TỔNG QUAN CÓ HỆ THỐNG, GIAI ĐOẠN 2010-2020
Authors: Ly Tran Thi , Nhu Dang Duc , Thang Tran Quoc , Luong Duong Duy
Published: July 2021

Đặt vấn đề: Mức độ hài lòng của người bệnh (NB) là thước đo đánh giá uy tín, chất lượng cung cấp dịch vụ của các cơ sở y tế. Do vậy, xác định mức độ hài lòng của NB đến khám, điều trị tại các cơ sở y tế và các yếu tố liên quan trong bối cảnh hiện nay là vô cùng cần thiết và có ý nghĩa, nhằm cung cấp bằng chứng, làm cơ sở đề xuất giải pháp nâng cao chất lượng khám, chữa bệnh (KCB).Mục tiêu: Nghiên cứu tổng quan có hệ thống nhằm cung cấp thông tin tổng quát về mức độ hài lòng và các yếu tố liên quan của NB đến khám và điều trị tại các cơ sở y tế ở Việt Nam, phân tích điểm mạnh, điểm yếu và những thiếu hụt cần bổ sung cho các nghiên cứu tiếp theo về vấn đề này.Phương pháp: Tổng quan tài liệu có hệ thống (Systematic review)Kết quả: Tìm kiếm và phân tích 30 công trình nghiên cứu trong nước liên quan đến mức độ hài lòng của NB. Kết quả cho thấy, các nghiên cứu về mức độ hài lòng của NB được thực hiện trên nhiều đốitượng và địa điểm với mức độ hài lòng khác nhau.Kết luận: Mức độ hài lòng của NB khá cao (trung bình đạt trên 70%, điểm trung bình trên 3,5). Các lĩnh vực có tỷ lệ NB hài lòng cao gồm: Thái độ ứng xử, năng lực chuyên môn của NVYT; sự minhbạch thông tin và thủ tục khám bệnh, điều trị. Các lĩnh vực có tỷ lệ NB hài lòng chưa cao như: Khả năng tiếp cận; kết quả cung cấp dịch vụ và cơ sở vật chất và phương tiện phục vụ NB. Về các yếu tố liên quan: Sự hài lòng của NB chưa/ít có liên quan đến các yếu tố nhân khẩu học như: Tuổi, nghề nghiệp, trình độ học vấn, đối tượng KCB (có BHYT/không có BHYT). Các yếu tố liên quan nhiều đến mức độ hài lòng của NB bao gồm: Thời gian chờ đợi KCB, thời gian điều trị, cơ sở vật chất.
Investigation of Immunomodulatory Activity of Methanolic Extract and Isolated Compound of Pavonia odorata Roots in Mice
Authors: Singune Shantilal , Vaghela Jai S
Published: July 2021

The immune system plays a role in protective mechanism against the foreign and its modulation either suppression or stimulation was implicated in several human diseases like infection, inflammatory and autoimmune diseases, etc. For which several allopathic immunomodulators have been used for treatment, exhibit potential side effects. Biological active compounds from natural sources used in the treatment of an immune-related disorder. Being the importance of natural compound. The present study was undertaken to evaluate the immunomodulatory activity of methanolic roots extract of Pavonia odorata and isolated compounds from Pavonia odorata. Immunomodulatory activities were determined by in vivo models- delayed-type hypersensitivity (DTH), carbon clearance assay (Phagocytic index), and cyclophosphamide-induced immunosuppression. The methanolic extracts of Pavonia odorata and isolated compound, in the concentration range 5, 10, 25, 50 and 100µg were also tested for phagocytic effect on neutrophils using the in vitro models-nitroblue tetrazolium (NBT) dye test. Mice treated with dose i.e. 200mg/kg (Group III) and dose 370mg/kg (Group-IV) increase response in footpad edema was found to be statistically significant (*p˂ 0.05), (**p˂ 0.01) and showed statistically significant (*p˂ 0.05) increase in WBC and platelets count and phagocytic index when compared to cyclophosphamide (20mg/kg) treated and control-treated mice. In cyclophosphamide induces immunosuppression, methanolic roots extract Pavonia odorata and isolated compound showed significant (P < 0.001) increase total differential leucocytes count DLC and hematological parameters RBCs, Hb, Platelets and WBCs significant increases when compared with cyclophosphamide group. Methanolic extract of Pavonia odorata and isolated compound in the concentration range 5, 10, 25, 50 and 100µg also showed significant (p˂ 0.001) phagocytic effect on neutrophils in the parameter studied. The present experimental finding demonstrated that isolated compound has superior immunomodulatory activity then methnolic extract of Pavonia odorata this effect presumably due to the greater ability of isolated compound to boost the innate and adaptive immune system.
Evaluation of Cytotoxicity of normal Vero and Anticancer Activity of Human Breast Cancer Cell Lines by Aqueous Unripe Fruit Extract of Solanum torvum
Authors: Shanthi D. , Saravanan R.
Published: July 2021

In the present study aqueous extract of Solanum torvum unripe fruits was used to evaluate its cytotoxic effect and anticancer activity through in vitro studies by 3-(4, 5 dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide- MTT assay) on Normal VERO cell line and MCF-7 (Human breast adenocarcinoma cell line). Aqueous extracts of Solanum torvum unripe fruits was found to be effective in the prevention of cell proliferation by breast adenocarcinoma cell lines at 1000 µg/ml from the results obtained during 24 hours of incubation.
Stability Indicating RP-HPLC Method Development and Validation for the Determination of Solifenacin Succinate in Bulk and its Pharmaceutical Formulation
Authors: Tanuja A. , Ganapaty S. , S N Murthy Varanasi
Published: July 2021

The current work aims to establish a novel and advanced reverse phase isocratic liquid chromatography system followed by validation and to conduct stability analysis in active pharmaceutical ingredients and formulations for the quantification of Solifenacin Succinate. The optimized elution was achieved with column Sunfire C8 (4.6 x 150mm, 5µm), using the mobile phase of Buffer: Methanol: Acetonitrile in the composition ratio of 45:45:10 v/v. The wavelength of detection was selected as 220nm with 1.0ml/ min flow rate and 30μl injection volume. The retention time of Solifenacin Succinate was found 2.94 min respectively. The method developed has been validated for various analytical parameters according to ICH guidelines. The Linearity was attained at 20 to100 μg/ml of concentration range. The established method was proved as reproducible. The Assay was obtained as 100.40%. The degradation studies were carried out at all degradative conditions and the results of degradation studies denote that the current method was specific, reliable, and economical. Hence, the developed method can be applied for the qualitative and quantitative determination of the selected drug and its commercial formulations.
The use of Topical Platelet-Rich Plasma on Second Degree Burn Injury: An Experimental study
Authors: Paramita Desiree A , Paramita Deryne A , Siregar E S , Syahputra R A
Published: July 2021

Burn is a tissue injury due to extreme heat, electrical, radiation, corrosive chemical and friction sources. It is one of the major reasons of death and inability and accounts the fourth cause of injuries worldwide. Partial thickness burn damages the epidermis and part of the dermis. Platelet-rich plasma (PRP) has been used in several surgeries and showed a positive result regarding the healing process. In this study, 20 Wistar rats were applied the PRP and 20 others were control Histopathological analysis was done confirming the partial-thickness burn injury. The mean of weight in the control group was 276.2(±14.00) grams compared to 278.7(±11.86) grams in the PRP group, having no statistical difference (p-value 0.609)
Molecular Docking Analysis of the T450A Mutation of the Gene rpoB Mycobacterium leprae from Leprosy Patients in Papua, West Papua and North Maluku, Indonesia
Authors: Maladan Yustinus , Krismawati Hana , Wahyuni Tri , Lorensi Hutapea Hotma Martogi , Rokhmad Muhammad Fajri , Parikesit Arli Aditya
Published: July 2021

Leprosy persists to be a health problem in Indonesia, especially in the provinces of North Maluku, West Papua and Papua. Early diagnosis and complete treatment with multidrug therapy (MDT) remain the key strategy for reducing the disease burden. One of the major components of MDT is rifampicin which in certain cases in several countries, M. leprae resistance to this drug issue has been reported albeit only a few. This research aimed to detect and analyze polymorphism in M. leprae rpoB gene that was isolated from leprosy patients in three provinces: North Maluku Province, West Papua Province and Papua Province, Indonesia. The identification of mutations in the M. leprae rpoB gene was carried out by aligning the results of DNA sequencing with the reference strain. The 3D structure of rpoB was derived using the Swiss Model. The T450A, S456L, and H451Y variants of RNA Polymerase B subunits were constructed using FoldX based on the wild-type structure. The structures were repaired, and protein stability was evaluated using foldX under the Yasara viewer. The QC of the rpoB M. leprae homology models was conducted with Ramachandran Plot modeling using PROCHECK. The difference in binding affinity between native protein and T450A, S456L, and H45I variants were analyzed using molecular docking. rpoB gene of M. leprae contains a mutation found in nucleotide of 1348 bp. The mutation triggered the conversion of the amino acid Threonine to Alanine in the amino acid to 450 rpoB subunit B. The structure of 3D RNA Polymerase Subunit B was constructed using rpoB Mycobacterium tuberculosis with PDB code 5UH5 as template. According to Ramachandran Plot, the percentage of residues in the most favored regions are 91.9%, and there was no significant number of residues in the disallowed regions. The results of molecular docking showed that the T450A variant had the same binding affinity with the native protein which was -8.9 kcal. Binding affinity on the S456L and H451Y variants increased by -7.3 kcal and -8.2 kcal, respectively. According to Molecular Docking analysis, T450A variant did not affect the energy binding between RNA polymerase and rifampicin.
Development of Dermatological Ointment based on Aloe arborescens juice
Authors: Svetlana Nikolaevna Glushchenko , Elena Alekseevna Mikhaylova , Aleksandrovich Kurkin Vladimir , Anna Alexandrovna Kochukova
Published: July 2021

The purpose of this study is to select the optimal ointment base for the creation of a soft dosage form, to develop methods for standardizing dermatological ointment based on Aloe arborescens juice and to study antibacterial activity. The choice of the optimal ointment base, ensuring the maximum therapeutic effect of the ointment, was made. Ointments were prepared in accordance with the physical-chemical properties of the components of the basics. The criterion for evaluating the effectiveness of the used ointment bases was the degree of release of the active substances (anthracene derivatives). During the studies, antimicrobial activity was determined in the in vitro experiment. The comparative determination of the antimicrobial activity of ointment bases and juice of Aloe arborescens was performed by direct diffusion into agar. To develop the method of quantitative determination, the absorption spectra of Aloe arborescens juice and ointment extraction with 60% ethyl alcohol were studied. The optical density of the solution on the spectrophotometer at a wavelength of 396nm was measured. The quantitative content of the total active substances in the ointment, calculated on barbaloin was determined by using UV spectrophotometry.
The effectiveness of Smartphone application-based education “Teman Diabetes” on clinical outcomes of Type-2 Diabetes mellitus patients
Authors: Hasanah Nidaul , Ikawati Zullies , Akbar Zainal Zainol
Published: July 2021

Introduction: Smartphone-based education has been proven to indirectly improve clinical outcomes of type-2 diabetes mellitus patients via practical disease management. “Teman Diabetes” is a new smartphone application that helps diabetics better manage their diabetes, and allows collaboration between diabetics, their inner circle, and medical professionals. However, there is still limited evidence about the effectiveness of “Teman Diabetes” on clinical outcomes of diabetic patients, especially type-2 diabetes mellitus. The objective of this research is to determine the positive effect of smartphone application-based education “Teman Diabetes” on the clinical outcomes of type-2 diabetes mellitus patients. The clinical outcomes measured were HbA1c and fasting plasma glucose levels. Method: Using a quasi-experimental design, a total of 83 participants were selected by consecutive sampling. Patients in the intervention group (n = 44) received "Teman Diabetes" when they assigned the informed consent form. Furthermore, they received a WhatsApp message containing a different link from the "Artikel" feature on the Teman Diabetes every week from the research team, from the second week to twelfth week. The control group (n = 39) only received standard therapy without any additional interventions. Patients in both groups were followed before and after 3 months (HbA1c and fasting plasma glucose levels). The statistical test used in this study is Mann-Whitney test for hypotheses and comparison of mean changes between groups; Wilcoxon test for comparison of mean changes between treatments; and analysis of covariance (ANCOVA) test for the effect of confounding variables. Results: After 3 months, the mean HbA1c reduction was greater in the intervention group by -0.7 ± 0.9% (P<0.001) than in the control group by -0.1 ± 1.1% (P=0.17). Also, the mean fasting plasma glucose reduction was greater in the intervention group by -25.0 ± 71.4mg/dl (P=.02) instead of in the control group by 7.6 ± 72.4mg/dl (P=0.78). It is concluded that smartphone application-based education “Teman Diabetes” give a significant positive effect on clinical outcomes among type-2 diabetes mellitus patients. The main features that contributed to the clinical outcome of this study were "Artikel", "Forum", and "Edukasi" feature.
Comparative Study of Chemerin and Inflammatory markers between Obese with Insulin Resistance and lean healthy women
Authors: Zaki Moushira , Kamal Sanaa , Elmotaleb A. Hussein Mona Abd , Tawfeek Hend M. , Girgiss Mina Wassef , Ibrahim Amr MM , Youness Eman R.
Published: July 2021

Background: Chemerin has been newly defined to be released from mature adipocytes and the chemerin concentrations in human serum augmented with obesity. There is a subclinical chronic low-grade inflammatory response where insulin resistance (IR) may develop. The aim of this study is to expound the prospective role of chemerin the in inflammation. Also, investigate relation between chemerin and serum lipid, glucose, body fat percentage, and metabolic parameters in obese and lean women with IR. Lymphocytes and neutrophils play a major role in inflammation and comprise the first line of defense against infection. The ratio of absolute neutrophil count to lymphocyte count, the neutrophil-to-lymphocyte ratio (NLR), determined as is a novel inflammatory biomarker utilized as a prognostic factor in numerous diseases Methods: This study was designed to investigate serum chemerin, NLR, and high-sensitive C-reactive protein (hsCRP) levels in 50 obese women with IR and 50 lean healthy women. Results: Obese group had significant higher levels of serum chemerin, NLR, hsCRP levels and metabolic parameters than lean one. Chemerin also correlated positively with NLR inflammatory marker and body mass index (BMI). Neutrophil‐to‐lymphocyte ratio was related to pro‐inflammatory Conclusion: The present study elucidates that chemerin levels are concomitant with obesity and IR and could play a role in the inflammation, having key aspects of metabolic syndrome.
In Vitro Antiviral Activity of Pinus merkusii (Pinaceae) Stem Bark and Cone against Dengue Virus Type-2 (DENV-2)
Authors: Muhammad Ansori Arif Nur , Fadholly Amaq , Proboningrat Annise , Hayaza Suhailah , Kuncoroningrat Susilo Raden Joko , Naw Sin War , Villar Posa Gabrielle Ann , Firza Yusrizal Yuhanis , Sibero Mada Triandala , Hari Sucipto Teguh , Soegijanto Soegeng
Published: July 2021

Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with IC50= 140.63 μg/mL and 73.78 μg/mL, CC50= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.
Pharmacognostical, Phytochemical and Fluorescence analysis of the plant Bougainvillea spectabilis (Willd.)
Authors: Kumar Das Prabhat , Vaghela Jai Singh , Badore Narendra
Published: July 2021

Objective: The aim of the study is to investigate the Phytochemical, Pharmacognostical, Fluorescence analysis and heavy metal testing of the plant Bougainvillea spectabilis (WILLD.). Methods: The plant powder was extracted with different solvents such as petroleum ether, chloroform, ethyl acetate, ethanol, and water. The different extracts were tested qualitatively for the identification of various phytochemical constituents. The plant powder was subjected to fluorescence analysis in daylight and in ultraviolet-light (254 nm and 365 nm) and heavy metal testing. Results: Water soluble extractive value was found to be higher than ethanol, Ethyl acetate, chloroform and petroleum ether. The total ash values were found to be higher followed by water soluble ash and acid insoluble ash. From the phytochemical screening result showed the presence of various phytochemical constituents such as alkaloid, glycoside, Carbohydrate, Flavonoids, Saponins, Terpenoids, Taninns and phytosterols. Fluorescence analysis of leaf powder of Bougainvillea spectabilis showed characteristic coloration with various chemicals. The presence of heavy metals like cobalt, mercury, nickel, silver and zinc were found negative. Conclusion: Thus the bioactive natural products in leaf extracts of Bougainvillea spectabilis can be used in the development of new pharmaceuticals that enhances therapeutic use.
Low-Cost Media for In-vitro Callus Development in
Catharanthus roseus (L) G. Don
Authors: S Saumya , S Shalini , S. Leelavathy , P. Deepa Sankar
Published: July 2021

Objective: The study focuses on formulating and validating low-cost media for in vitro callus induction and development in Catharanthus roseus. Methods: Percentage response, nature and mass of in vitro grown C. roseus callus was recorded for the conventional MS media with casein hydrolysate (1g/L) and three low-cost media supplemented with casein hydrolysate (1g/L). The effect of two hormonal combinations, viz., 1.5mg/L 2,4-D + 1mg/L Kin and 1mg/L BAP + 1mg/L NAA had been studied for callus development. Results: Two media combinations including MS (urea replacing NH4NO3) + table sugar (30g/L) + casein hydrolysate (1 g/L) + Food grade agar (FGA, 7g/L) and MS + table sugar (30g/L) + casein hydrolysate (1g/L) + sago (80g/L) was observed to take less number of days to callus induction, 11 and 12 days respectively, than the conventional MS media + casein hydrolysate (1g/L) + gelrite (2.2g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L), which took 13 days to callus induction. MS (urea replacing NH4NO3) + table sugar (30g/L) + casein hydrolysate (1g/L) + FGA (7g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L) gave rise to high callus mass, averaging to 12.351g, at the end of 4th subculture and also, high percentage of cost reduction per liter of media was observed compared to conventional MS media with casein hydrolysate. Conclusion: The present study indicated MS (urea replacing NH4NO3) + casein hydrolysate (1g/L) + FGA (7g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L) as a better media for developing and proliferating C. roseus callus In vitro.
Floating micro particles of Stavudine: An Exceptional approach for Gastric retention and Sustainable drug action
Authors: Kumar Ande Hemanth , Sudheer Preethi , M. Ashwini
Published: July 2021

Stavudine is synthetic analog of reverse transcriptase inhibitor possessing a short half-life of 0.8 to 1.5 hours. Therefore frequent administration of the medication is required which results in poor patient acceptability The following research work aims to prepare the floating microparticles of stavudine with an intention to increase the gastric retention time. Microparticles were prepared via emulsion solvent diffusion method utilizing Eudragit S 100 and Eudragit L 100 as the rate controlling polymers. The influence of these polymers and its compositions on various formulation parameters in addition to the in vitro release characteristics of the microspheres was investigated. The particle size of the prepared microparticles were found to be in the range of 108.25µm to 152.41µm. Free flowing particles which are spherical free flowing with a buoyancy ≥12 hour in the simulated gastric fluid were obtained. The drug content of the selected micro particles (F12) showed an encapsulation efficiency of up to 85.28±0.18%. In vitro release profiles of floating microspheres indicated a sustained drug release up to 14 hours. Thus, the present formulations could be a superior alternative to conventional oral therapy due to the sustained drug action.
UV Spectrophotometric Method Development and Validation of Luliconazole in Bulk and Formulation
Authors: Suryawanshi Anuja , Ansari Afaque , Kalshetti Mallinath
Published: July 2021

Objective: A new, simple, economical, sensitive, precise and reproducible UV visible spectrophotometric method was developed for the estimation of luliconazole in pure form and pharmaceutical formulation as per ICH guidelines. Method: A UV spectrophotometric method has been developed using methanol and water as solvent to determine the luliconazole in bulk and pharmaceutical dosage formulation. The λmax of luliconazole in methanol and water was found to be 297nm. Results: The drug was proved linear in the range of 3-15µg/ml and exhibited good correlation coefficient (R2= 0.9993) and excellent mean recovery (98-99%). The % RSD for intra-day and inter-day precision was found to be 1.051288 and 1.138658 respectively. The LOD and LOQ of Luliconazole was found to be 1.1168µg/ml and 3.3845µg/ml respectively. This method was successfully applied to luliconazole content in marketed brands and results were in good agreement with the label claims. Conclusion: The method was validated for linearity, precision, repeatability and reproducibility. The obtained results proved that the method can be employed for the routine analysis of luliconazole in bulks as well as in commercial formulations.
Development of cefdinir loaded Functionalized carbon Nanotubes dry powder Inhaler for the Treatment of cystic Fibrosis
Authors: Dhekale Krishnat D. , Kamble Ravindra N.
Published: July 2021

A dry-powder inhaler (DPI) carries medication to lungs as a dry powder, useful against respiratory diseases. The current research was endeavoured to examine the capabilities of Multi-walled carbon nanotubes (MWCNT) as a pulmonary transporter for directing cefdinir to cystic fibrosis (CF). Functionalized MWCNTs were loaded with cefdinir to formulate DPI (F-CEF FMWCNTs DPI) having efficient treatment against lung infections and were evaluated successfully. The outcomes demonstrated that cefdinir loaded FMWCNTs were non-toxic and accomplished 79.73 % entrapment with better flow properties. The optimized formulation had Mass Median Aerodynamic Diameter (MMAD), Fine particle fraction (FPF), and particle size of 3.45±0.09 μm, 58.52±1.06%, 5.25 ± 0.03 μm (CEF FMWCNT DPI) and 4. 29±0.16μm 38.74±1.02%, 7.54 ± 0.02 μm (C-DPI) respectively. The loaded nanotubes showed 72. 63 % release after 15 hours in a controlled manner. The outcome of work recognized a unique, simple, and stable product having improved drug loading and increased dispersibility of carbon nanotubes (CNTs) thus improved bioavailability at a lung infection place with less adverse actions.
Prevalence of 25-Hydroxyvitamin D Deficiency and its severity correlation with Acute Traumatic brain Injury in Indian Patients:
A Perspective Observation Study
Authors: Choudhary Ajay , Sharma Rajesh , Kumar Ashok , Kinja Kuldeep , Berwal Ravi , Sharma Swapnil
Published: July 2021

Objective: To study the prevalence of 25-hydroxyvitamin D deficiency pattern during three year (2017-2020) and severity correlation among individuals with acute traumatic brain injury (TBI). Methodology: Subjects with acute TBI admitted from June 1st, 2017 through June 30th, 2020 were recruited. 280 out of 445 met inclusion criteria. The demographic injury related details, assessment of 25 OH vitamin D and Glasgow Comma (GCS) score were done at the time of admission. Results: The year wise enrolled subjects were young with mean age of 28.39±0.86 years with males (73.3%) and female (23.7%), in first year, 27.77±5.35 years with males (81.67%) and female (18.33%), in second year and 23.04±7.10 years with males (88.57%) and female (11.42%), in third years. Mean value of 25(OH) vitamin D in subjects during three years were 23.78±11.79ng/mL, 21.65±12.53 ng/mL and 25.18±18.58ng/mL. The vitamin D deficiency levels in this study were tabulated as: deficient (level <20 ng/mL), insufficient (level 20–29.9ng/mL), and sufficient (level ≥30ng/mL). Which were found during three years as: In First year, Deficient (64.44%), Sufficient (11.11%), insufficient (24.44%), in second years, Deficient (88.33%), Sufficient (2.66%), insufficient (10.00%) and in third year Deficient (88.57%), Sufficient (1.42%), insufficient (10.00%). In which sufficient level were found to be decreased statistically significant with years with P value= 0.0001. The severity assessment through GCS score were found to be statistically increased with deficient levels with P values=0.0447, but found no significance, when comparison were done between years wise GCS score and levels of vitamin D. Conclusion: The study found decreased prevalence of vitamin D deficiency levels with increased severity. Therefore it should be routinely screened and treated as indicated.
Variabilidad genética de dos subpoblaciones de Cuyes (Cavia porcellus) nativos del sur del Ecuador
Authors: Rosales-Jaramillo Cornelio , Nieto-Escandón Pedro , Román-Bravo Rafael , Aranguren-Méndez José Atilio
Published: July 2021

La creciente necesidad actual de conservar los recursos zoogenéticos locales como fuentes de variabilidad genética (VG), plantea la necesidad de realizar investigaciones que ayuden a conocer su estado actual; por ello, se estudió la VG de dos subpoblaciones nativas de cuyes (Cavia porcellus) denominadas: Azuay y Cañar, geográficamente ubicadas al sur de Ecuador. Para su caracterización se utilizaron seis marcadores microsatélites de ADN, muestreándose 50 animales de cada subpoblación y un tercer grupo de 50 animales de origen peruano como grupo externo para comparación. De los seis marcadores usados, cinco pudieron ser amplificados; se encontró un número de alelos considerable (9,8) en la población general y un valor de (7,02) igual en ambas poblaciones con un alto grado de polimorfismo (PIC = 0,7035); dos loci estudiados, el MS I en ambas subpoblaciones y MS III en Azuay no se encontraron en equilibrio HW. La VG variabilidad fue alta en ambas subpoblaciones (Ho 0,694), así como un cierto grado de diferenciación genética (GST = 0,066), existiendo dos alelos privados con frecuencias superiores al 10 % (MS IV, Azuay 301 pb y 297 pb en Cañar), dando indicio de constituirse como marcadores de raza. La distancia genética entre subpoblaciones Azuay y Cañar es media (0,17), no obstante, resultó ser superior a las encontradas entre cada una de éstas y la población Perú. La realidad genética encontrada sugiere la necesidad de intervenir sobre estas poblaciones con la finalidad de conservar el material genético nativo incorporando un manejo sostenible del recurso zoogenético.
Nudos críticos de procesos gerenciales en unidades productivas de Cuyes de engorde en Milagro, Ecuador
Authors: Castro-García Alex , Nava Juan Carlos
Published: July 2021

Este estudio propone identificar los nudos críticos (NC) en los procesos gerenciales (PG) en unidades productivas (UP) de Cuyes de Engorde (CE) en Milagro, Provincia de Guayas, Ecuador, siendo la cría de C una actividad de importancia en diferentes zonas rurales. Por lo tanto, se visitó la mencionada zona, aplicando un cuestionario socio-económico como instrumento de recolección de datos; la población y la muestra estuvieron constituidas por 260 Productores (P) de CE. La investigación fue de carácter explicativa, según el diseño no experimental, de campo. Los NC que limitaron los PG en las UP de CE fueron: deficiente toma de decisiones (DTD), ausencia del presupuesto (AP), no llevan registros (NR) y no programan las actividades a realizar (NPA). Los P al identificar los NC deberían analizar cuáles son los factores que inciden en la producción de CE; planificando, revisando que necesidades existen, realizado ajustes, utilizando registros, con la necesidad de mejorar el manejo, la productividad y los ingresos netos; tomando en cuenta los costos de producción, con la elaboración del presupuesto, con supervisión y ajustes. Pese a que los P están realizando diferentes actividades relacionadas con los CE y las consideraron importantes, se detectó un desconocimiento en definir estrategias para luego tomar decisiones en beneficio de la UP, de acuerdo a cada situación real que se presente, con estrategias definidas previamente para buscar obtener mejores rendimientos.
Optimize the Learning State: Techniques and Habits
Authors: Peper Erik , Wilson Vietta
Published: July 2021

The use of online learning or consulting for biofeedback and neurofeedback will continue long after the COVID-19 pandemic. The authors reviewed findings of the effectiveness of online presentations and recommendations on how to enhance the online learner's ability to engage by using pre-performance routines or habits. This approach is derived from coaching athletes to achieve peak performance and includes environment cueing and personal cueing as strategies to enhance performance.
ANALISIS PEMAHAMAN KONSEP KELILING DAN LUAS SEGITIGA PESERTA DIDIK KELAS VIII SMP IT NURUL HUDA KEEROM BERDASARKAN TAKSONOMI BLOOM
Authors: Raldi Raldi , Rofiki Ahmad Arif , Lestari Nining Puji
Published: July 2021

Berdasarkan hasil belajar peserta didik kelas VIII SMP IT Nurul Huda Keerom pada materi segitiga sebagian besar kurang memuaskan. Hal ini membuktikan bahwa pemahaman konsep keliling dan luas segitiga masih rendah dan pemahaman setiap peserta didik berbeda-beda. Penelitian ini bertujuan untuk mengetahui (1) pemahaman konsep keliling dan luas segitiga peserta didik kelas VIII SMP IT Nurul Huda Keerom dan (2) Faktor-faktor yang mempengaruhi pemahaman konsep peserta didik kelas VIII SMP IT Nurul Huda Keerom. Jenis penelitian ini adalah penelitian deskriptif kualitatif. Pendekatan penelitian menggunakan pendekatan pedagogi. Teknik analisis data menggunakan reduksi data, penyajian data, dan penarikan kesimpulan. Penyajian keabsahan datanya yaitu kepercayaan, keteralihan, kebergantungan, dan kepastian. Hasil penelitian menunjukkan bahwa (1) Subjek kemampuan matematika tinggi berada pada tahapan mengaplikasikan, subjek kemampuan matematika sedang berada pada tahapan mengaplikasikan, subjek kemampuan matematika rendah hanya berada pada level tahapan mengingat berdasarkan Taksonomi Bloom. (2) Faktor internal yang mempengaruhi berkemampuan matematika tinggi memiliki kesiapan dalam belajar dan memiliki sedikit minat dalam belajar. Subjek berkemampuan matematika sedang memiliki kesiapan dalam belajar dan memiliki minat untuk belajar. Subjek berkemampuan matematika rendah selalu memiliki kesiapan dalam belajar dan memiliki motivasi dari dalam diri untuk belajar. Sedangkan faktor eksternalnya semua subjek mendapatkan motivasi dari orang tua, guru, dan sekolah.
Ecological aspect of using road-rail vehicles for
shunting operations
Authors: Pielecha Jacek , Merkisz-Guranowska Agnieszka , Andrzejewski Maciej , Daszkiewicz Paweł , Stawecka Hanna
Published: July 2021

W artykule dokonano oceny korzystności zastosowania pojazdów szynowo-drogowych
w prowadzeniu prac manewrowych na torach, zamiast stosowanych powszechnie,
spalinowych lokomotyw manewrowych. Skupiono się głównie na aspektach ekologicznych
– emisji toksycznych substancji w spalinach. Przedstawiono w nim między
innymi przykładowe wyniki badań emisji zanieczyszczeń z wybranych pojazdów
transportu masowego, użytkowanych na polskich liniach kolejowych. W celu dokonania
analizy uciążliwości środowiskowej tych pojazdów wykonano pomiary toksyczności
spalin w warunkach testu badawczego (ISO 8178-F) oraz, co jest znacznie
istotniejsze, podczas rzeczywistego użytkowania pojazdów – przy wykorzystaniu mobilnej
aparatury naukowo-badawczej.
Fire safety of rolling stock within the meaning of
Polish and European legislation
Authors: Wojciechowska Estera , Szczurek Maria
Published: July 2021

Głównym celem ochrony w przypadku wystąpienia pożaru na pokładzie pojazdu
szynowego jest umożliwienie pasażerom i załodze ewakuacji z pojazdu w obszar
możliwie największego bezpieczeństwa. Czas przebywania pasażerów w pojeździe
powinien być jak najkrótszy.
Dzięki materiałom ograniczającym palność i dostępnych na pojazdach szynowych
gaśnicom pożar rozprzestrzenia się powoli a zagrożenie występuje tylko w
bezpośrednim otoczeniu ogniska pożaru. Działanie pożaru jest tym samym mocno
ograniczone lokalnie.
Superkondensatory hybrydowe dla komunikacji miejskiej
Authors: Bramson Edward , Staroński Krzysztof
Published: July 2021

W artykule przedstawiono budowę najnowszych rozwiązań superkondensatorów
dwuokładzinowych oraz Li-ion. Analiza porównawcza ich parametrów
znamionowych pozwoliła na przedstawienie potencjału wykorzystania w
trakcji elektrycznej. W kolejnym rozdziale przedstawiono wyniki badań
symulacyjnych, które obrazują wpływ różnic parametrów badanych
superkondensatorów na efektywność ich wykorzystania. Następnie
zamieszczono wyniki badań eksperymentalnych, mających na celu zbadanie
stałości parametrów superkondensatorów EDLC oraz LIC. Zamieszczono
opis wykonywanych prób, metodykę wyznaczania szukanych parametrów
oraz wykresy ilustrujące wyniki badań i obliczeń.
Badania właściwości taboru dotyczącej zgodności
z systemami wykrywania pociągów
Authors: Olejniczak Tomasz , Woźniak Krystian , Kowalski Michał
Published: July 2021

W artykule przedstawiono źródła powstawania zakłóceń elektromagnetycznych
mogących wpływać na poprawne działanie urządzeń sterowania ruchem kolejowym.
Zestawiono obowiązujące w Polsce dopuszczalne poziomy występowania
zakłóceń dla wybranych typów czujników. Badania wykonywane na infrastrukturze
kolejowej wymagają odpowiedniego przygotowania zarówno merytorycznego,
badanego obiektu jak i stanowiska pomiarowego. Badania wykonuje się zgodnie z
przygotowanym scenariuszem uwzględniającym możliwie wszystkie sytuacje, w
których badany pojazd może generować zakłócenia powodujące niestabilną pracę
czujników, takie jak rozruch czy hamowanie. Przedstawiono przykładowe wyniki
pomiarów wraz z kryteriami oraz sposoby przeprowadzania badań, a także szczegółowe
wymagania dotyczące aparatury pomiarowej.
La opacidad de la oralidad en el aula de clase: un reto en la docencia
Authors: Tafur-Muñoz Sandra Patricia , Armesto-Argüelles Nelsy Jhoanní
Published: July 2021

El desarrollo de la oralidad en el aula de clase es una de las realidades que enfrenta el docente investigador, es por ello que este artículo de reflexión reconoce que, desde la perspectiva lingüístico-discursiva, el orden pedagógico e investigativo puede comprender y resignificar la importancia de la oralidad en el proceso formativo del estudiante. Esta propuesta se enfoca desde la investigación pedagógica de las actividades discursivas de la lengua y el análisis crítico del discurso (ACD) a fin de teorizar y generar dinámicas que, a corto y largo plazo, reduzcan las asimetrías sociales a través del lenguaje al tiempo que se disminuyen los niveles de glosofobia resultado de las relaciones de poder en el aula.