bluuebunny/crossref_metadata_2025 huggingface dataset:
Burden and factors influencing intestinal parasitic infections among food handlers in Gondar City, Northwest Ethiopia
Authors: Getie Michael , Belay Gizeaddis , Amare Azanaw , Abebe Wondwossen , Deress Teshiwal
Published: June 2024

Background

Intestinal parasitic infections pose significant global health challenges, particularly in developing countries. Asymptomatic infections often present a considerable burden with food handlers serving as potential carriers. In Ethiopia, the prevalence of these parasites varies across regions, and accurate data in the study area is lacking. Therefore, this study aimed to investigate the prevalence of intestinal parasites among food handlers working in hotels and restaurants in Gondar City, Northwest Ethiopia.

Methods

A cross-sectional study collected stool samples from food handlers alongside a structured questionnaire gathering socio-demographic and hygiene practice information. Stool specimens were screened for intestinal parasites using direct wet mount and formol-ether concentration techniques. The collected data were checked for completeness, entered into EpiData software version 3.1, and exported to SPSS version 20 for analysis. A multivariable logistic regression analysis was deemed statistically significant if the p-value was less than 0.05.

Results

A total of 257 food handlers working in hotels and restaurants in Gondar City participated in the study. Of these, 33.5% (86/257) were found positive for one or more intestinal parasites, with a 95% confidence interval (CI) of 28.0–39.5%. The study identified nine types of intestinal parasites, with E. histolytica/dispar (8.2%, 21/257) and Ascaris lumbricoides (6.6%, 17/257) being the predominant parasites, followed by hookworm (3.5%, 9/257) and S. mansoni (2.3%, 6/257). The prevalence of mixed infections was 9.3% (24/257). A significant association was observed between intestinal parasitic infection and the educational level of food handlers.

Conclusion

In this study, a high prevalence of intestinal parasites was detected indicating poor hygiene practices of the food handlers at the study site. Even the prevalence of mixed infections was high. Regular training, strict adherence to personal hygiene and food-handling practices, and routine inspections and medical checkups for food handlers are crucial.
Engagement in a pilot produce prescription program in rural and urban counties in the Southeast United States
Authors: Owens Caroline E. , Cook Miranda , Reasoner Tammy , McLean Aleta , Webb Girard Amy
Published: June 2024

Introduction

In the United States, over one in every ten households experiences food insecurity. Food insecurity is associated with often co-occurring adverse health consequences, including risk for obesity, type 2 diabetes, and hypertension. Within the “Food is Medicine” intervention space, Produce Prescription Programs (PRx) seek to alleviate food insecurity and improve diet and health outcomes by leveraging access to produce through healthcare organizations. Though these programs are burgeoning across the United States, research surrounding their implementation and outreach is limited.

Methods

This study evaluates the implementation, reach, engagement, and retention of a PRx program piloted in two regions of Georgia (US) from 2020 to 2022. The study included 170 people living with one or more cardiometabolic conditions recruited from clinical sites in metropolitan and rural areas. The program provided pre-packaged produce boxes and nutrition education over six months. We examine participants’ baseline demographics, food security status, dietary patterns, and loss to follow-up across contexts (metropolitan and rural). We employ regression analyses and model comparison approaches to identify the strongest predictors of loss to follow-up during the pilot period.

Results

In the pilot period of this program, 170 participants enrolled across rural and metropolitan sites. Of these, 100 individuals (59%) remained engaged for the six-month program. While many individuals met the target criteria of living with or at-risk of food insecurity, not all lived with low or very low food security. Metropolitan participants, males, and those with children in the household had significantly higher odds of loss to follow-up compared to rural participants, females, and those without children in the household. No other significant demographic or household differences were observed.

Discussion

This study demonstrates the potential of PRx programs to enhance food and nutrition security and cardiometabolic health in metropolitan and rural clinical settings. Future research should focus on addressing barriers to engagement and expanding the reach, impact, and sustainability of PRx programs across diverse contexts.
The FITNESS study: longitudinal geriatric assessment, treatment toxicity, and biospecimen collection to assess functional disability among older adults with lung cancer
Authors: Grogan Madison , Hoyd Rebecca , Benedict Jason , Janse Sarah , Williams Nyelia , Naughton Michelle , Burd Christin E. , Paskett Electra D. , Rosko Ashley , Spakowicz Daniel J. , Presley Carolyn J.
Published: June 2024

Introduction

Older adults with chronic disease prioritize functional independence. We aimed to describe the feasibility of capturing functional disability and treatment toxicity among older adults with lung cancer using a longitudinal comprehensive geriatric assessment (CGA) and molecular biomarkers of aging.

Methods

This prospective study included adults ≥60 years with any newly diagnosed non-small-cell lung cancer. Participants were recruited from central Ohio (2018–2020). Study assessments included the Cancer and Aging Research Group CGA (CARG-CGA), short physical performance battery (SPPB), and the blessed orientation-memory concentration (BOMC) test at baseline, 3, 6, and 12 months. Activities of daily living (ADLs) and instrumental ADLs (IADLs), quality of life (QoL, PROMIS 10), and treatment toxicity were captured monthly. Stool and blood were collected to characterize the gut microbiome and age-related blood biomarkers.

Results

This study enrolled 50 participants with an average age of 71.7 years. Ninety-two percent of participants were Caucasian, 58% were male, and all were non-Hispanic. Most had advanced stage (stage III/IV: 90%; stage I/II: 10%), with adenocarcinoma the predominant histologic subtype (68% vs. 24% squamous). First-line treatments included chemotherapy (44%), immune checkpoint inhibitors (ICIs, 22%), chemotherapy and ICIs (30%), or tyrosine kinase inhibitors (4%). The median baseline CARG toxicity score was 8 (range 2–12). Among patients with treatment-related toxicity (n = 49), 39 (79.6%) cases were mild (grade 1–2), and 10 (20.4%) were moderate to severe (≥ grade 3). Treatment toxicity was greater among those with a CARG score ≥8 (28.0% vs. 13.6%). Higher IADL independence, QoL, and SPPB scores at baseline were positively associated with Candidatus Gastranaerophilales bacterium, Lactobacillus rogosae, and Enterobacteria phage P4. Romboutsia ilealis, Streptococcus, and Lachnoclostridium sp An138 and T cell lag3 and cd8a were associated with worse IADLs, QoL, and SPPB scores at baseline.

Discussion

A longitudinal CGA and biomarker collection is feasible among older adults undergoing lung cancer treatment. Gut microbe and T cell gene expression changes correlated with subjective and objective functional status assessments. Future research will test causality in these associations to improve outcomes through novel supportive care interventions to prevent functional disability.
Assessing the accessibility and quality of mobile health applications for the treatment of obesity in the German healthcare market
Authors: Stapelfeldt Pia M. , Müller Sina A. M. , Kerkemeyer Linda
Published: June 2024

Introduction

Overweight and obesity are among the most prevalent health problems worldwide leading to various diseases and having a significant impact on the healthcare system. In Germany, the prevalence of obesity among adults is 19%. Mobile health applications offer a new approach to treatment and prevention and have been proven effective in previous studies. However, it is essential to investigate the availability and quality of these digital applications. The aim of this systematic assessment is to evaluate the accessibility and quality of digital health applications in German language designed to treat obesity.

Methods

In January 2024, a systematic search for mobile health applications was conducted on both the Google Play Store and Apple App Store. Just those apps available in German for both iOS and Android were considered acceptable. The German Mobile Application Rating Scale (MARS-G) was used to assess the quality of the apps. The content of mobile health applications was evaluated using the guideline from the German Obesity Society for the treatment of obesity. The characteristics of the apps were summarized and presented, and the results were analyzed using descriptive statistics and presented in tables.

Results

After screening, ten apps were included in the review. The apps varied in terms of calorie tracking, individual workout plans, educational aspects, nutritional plans, and exercises for behavioral change. On average, 6.4 out of 12 items of the German Obesity guideline recommendations were fulfilled. The MARS score (possible range from 1–5) reached a mean of 3.39 (SD = 0.39). The section “Engagement” had the lowest quality score with a mean of 3.14 (SD = 0.57), while the section “Aesthetics” achieved the highest mean of 3.57 (SD = 0.52).

Discussion

Most German mobile health applications for managing obesity meet some guideline recommendations. They demonstrate adequate to good quality according to the MARS score. Assessing the quality of mobile health applications can be challenging for patients, despite being easily accessible and low-threshold. However, such digital health applications, reimbursed by the German SHI, offer evidence-based information, even if access can be associated with higher hurdles.
Compilation of resources on subcellular localization of lncRNA
Authors: Choudhury Shubham , Rathore Anand Singh , Raghava Gajendra P. S.
Published: June 2024

Long non-coding RNAs (lncRNAs) play a vital role in biological processes, and their dysfunctions lead to a wide range of diseases. Due to advancements in sequencing technology, more than 20,000 lncRNA transcripts have been identified in humans, almost equivalent to coding transcripts. One crucial aspect in annotating lncRNA function is predicting their subcellular localization, which often determines their functional roles within cells. This review aims to cover the experimental techniques, databases, and in silico tools developed for identifying subcellular localization. Firstly, we discuss the experimental methods employed to determine the subcellular localization of lncRNAs. These techniques provide valuable insights into the precise cellular compartments where lncRNAs reside. Secondly, we explore the available computational resources and databases contributing to our understanding of lncRNAs, including information on their subcellular localization. These computational methods utilize algorithms and machine learning approaches to predict lncRNA subcellular locations using sequence and structural features. Lastly, we discuss the limitations of existing methodologies, future challenges, and potential applications of subcellular localization prediction for lncRNAs. We highlight the need for further advancements in computational methods and experimental validation to enhance the accuracy and reliability of subcellular localization predictions. To support the scientific community, we have developed a platform called LncInfo, which offers comprehensive information on lncRNAs, including their subcellular localization. This platform aims to consolidate and provide accessible resources to researchers studying lncRNAs and their functional roles (http://webs.iiitd.edu.in/raghava/lncinfo).
High-precision apple recognition and localization method based on RGB-D and improved SOLOv2 instance segmentation
Authors: Tang Shixi , Xia Zilin , Gu Jinan , Wang Wenbo , Huang Zedong , Zhang Wenhao
Published: June 2024

Intelligent apple-picking robots can significantly improve the efficiency of apple picking, and the realization of fast and accurate recognition and localization of apples is the prerequisite and foundation for the operation of picking robots. Existing apple recognition and localization methods primarily focus on object detection and semantic segmentation techniques. However, these methods often suffer from localization errors when facing occlusion and overlapping issues. Furthermore, the few instance segmentation methods are also inefficient and heavily dependent on detection results. Therefore, this paper proposes an apple recognition and localization method based on RGB-D and an improved SOLOv2 instance segmentation approach. To improve the efficiency of the instance segmentation network, the EfficientNetV2 is employed as the feature extraction network, known for its high parameter efficiency. To enhance segmentation accuracy when apples are occluded or overlapping, a lightweight spatial attention module is proposed. This module improves the model position sensitivity so that positional features can differentiate between overlapping objects when their semantic features are similar. To accurately determine the apple-picking points, an RGB-D-based apple localization method is introduced. Through comparative experimental analysis, the improved SOLOv2 instance segmentation method has demonstrated remarkable performance. Compared to SOLOv2, the F1 score, mAP, and mIoU on the apple instance segmentation dataset have increased by 2.4, 3.6, and 3.8%, respectively. Additionally, the model’s Params and FLOPs have decreased by 1.94M and 31 GFLOPs, respectively. A total of 60 samples were gathered for the analysis of localization errors. The findings indicate that the proposed method achieves high precision in localization, with errors in the X, Y, and Z axes ranging from 0 to 3.95 mm, 0 to 5.16 mm, and 0 to 1 mm, respectively.
Blebbistatin as a novel antiviral agent targeting equid herpesvirus type 8
Authors: Li Liangliang , Cui Xiu , Yu Yue , Sun Qi , Li Wenjing , Li Yubao , Li Shuwen , Chen Li , Khan Muhammad Zahoor , Wang Changfa , Wang Tongtong
Published: June 2024

Introduction

Equid herpesvirus type 8 (EqHV-8) poses a significant threat to equine health, leading to miscarriages and respiratory diseases in horses and donkeys, and results in substantial economic losses in the donkey industry. Currently, there are no effective drugs or vaccines available for EqHV-8 infection control.

Methods

In this study, we investigated the in vitro and in vivo antiviral efficacy of Blebbistatin, a myosin II ATPase inhibitor, against EqHV-8.

Results

Our results demonstrated that Blebbistatin significantly inhibited EqHV-8 infection in Rabbit kidney (RK-13) and Madin-Darby Bovine Kidney (MDBK) cells in a concentration-dependent manner. Notably, Blebbistatin was found to disrupt EqHV-8 infection at the entry stage by modulating myosin II ATPase activity. Moreover, in vivo experiments revealed that Blebbistatin effectively reduced EqHV-8 replication and mitigated lung pathology in a mouse model.

Conclusion

Collectively, these findings suggest that Blebbistatin holds considerable potential as an antiviral agent for the control of EqHV-8 infection, presenting a novel approach to addressing this veterinary challenge.
Seroprevalence of infectious pathogens of zoonotic and veterinary importance in wild ruminants from Slovenia
Authors: Žele Vengušt Diana , Krt Brane , Blagus Rok , Vengušt Gorazd , Bandelj Petra
Published: June 2024

Introduction

Wildlife represents an increasingly important source of pathogens of medical and veterinary importance. Surveillance in wildlife offers an insight on current epidemiological status of selected pathogens and help to prevent spillovers to humans and livestock.

Material and methods

Our study included 312 wild ruminants belonging to five species: Roe deer (n = 134), red deer (n = 113), Alpine chamois (n = 53), European mouflon (n = 10) and Alpine ibex (n = 2). Seven pathogens that may have profound effect on human/livestock health and economic viability of the farms were tested using serological methods.

Results

Antibodies against Toxoplasma gondii, Neospora caninum, Coxiella burnetii, Brucella spp., Chlamydophila abortus, Mycobacterium avium subsp. paratuberculosis (MAP) and Mycobacterium bovis were detected in 34.62% (108/312), 0.96% (3/312), 2.24% (7/312), 0, 0.96% (3/312), 0, 0.64% (2/312) of animals tested, respectively. Because of low prevalences, risk factors were assessed only for T. gondii. Sex (female>male) and species (roe deer>red deer, roe deer>Alpine chamois) were significantly associated with the T. gondii positive outcome, while age was not.

Discussion

Adult males had the lowest T. gondii prevalence which offers future research opportunities. The lower seroprevalence of most investigated pathogens suggests game meat, if properly cooked, as being relatively safe for human consumption. This is the first study investigating the seroprevalence and associated risk factors of selected pathogens in wild ruminants in Slovenia.
Comparison of the performance of SAG2, GRA6, and GRA7 for serological diagnosis of Toxoplasma gondii infection in cats
Authors: Sabukunze Serges , Gu Haorong , Zhao Lin , Jia Honglin , Guo Huanping
Published: June 2024

Toxoplasmosis is an important zoonotic disease caused by Toxoplasma gondii that can infect almost all warm-blooded animals worldwide, including humans. The high prevalence of T. gondii infection and its ability to cause serious harm to humans and animals, especially immunodeficient individuals, make it a key public health issue. Accurate diagnostic tools with high sensitivity are needed for controlling T. gondii infection. In the current study, we compared the performance of recombinant SAG2, GRA6, and GRA7 in ELISA for the serological diagnosis of T. gondii infection in cats. We further investigated the antigenicity of recombinant dense granule protein 3 (rGRA3), rGRA5, rGRA8, and rSRS29A expressed in a plant-based, cell-free expression system for detecting antibodies in T. gondii-infected cats. In summary, our data suggest that GRA7 is more sensitive than the other two antigens for the serodiagnosis of T. gondii infection in cats, and GRA3 expressed in the cell-free system is also a priming antigen in serological tests for detecting T. gondii infection in cats.
Synthesis of Circular Antenna Arrays for Achieving Lower Side Lobe Level and Higher Directivity Using Hybrid Optimization Algorithm
Authors: Mittal Vikas , Sharma Kanta , Thangarasu Narmadha , Sarat Udandarao , Hourani Ahmad , Salgotra Rohit
Published: June 2024

Circular antenna arrays (CAAs) find extensive utility in a range of cutting-edge communication applications such as 5G networks, the Internet of Things (IoT), and advanced beamforming technologies. In the realm of antenna design, the side lobes levels (SLL) in the radiation pattern hold significant importance within communication systems. This is primarily due to its role in mitigating signal interference across the entire radiation pattern’s side lobes. In order to suppress the subsidiary lobe, achieve the required primary lobe orientation, and improve directivity, an optimization problem is used in this work. This paper introduces a method aimed at enhancing the radiation pattern of CAA by minimizing its SLL using a Hybrid Sooty Tern Naked Mole-Rat Algorithm (STNMRA). The simulation results show that the hybrid optimization method significantly reduces side lobes while maintaining reasonable directivity compared to the uniform array and other competitive metaheuristics.
Seismic Performance of Steel-Reinforced High Strength Concrete Joints Considering Bond Slip Effect
Authors: Liu Wei , Guo Jiacheng
Published: June 2024

This study presents a solution for push-out failure for the staged bond-slip constitutive relationship between the structural steel and high strength concrete, taking into account the concrete strength grade, anchoring length, and stirrup ratio. The critical point coordinates for different stages are determined by the tests of 14 steel-reinforced high strength concrete (SRHSC) specimens. It is observed that with the increase in the concrete strength grade and anchorage length, the ultimate load of the specimens increased significantly, but the influence on the residual bond strength was not significant. The effect of the stirring ratio was mainly manifested in a slight increase in the initial bond strength. The formula for calculating SRHSC characteristic bond strength and characteristic slip value is established, and the bond-slip constitutive relation of SRHSC is proposed based on the tests. The material constitutive model considering the effect of bond-slip is implanted into the software in the case of the ABAQUS finite element platform. The material is applied to the numerical simulation analysis of the SRHSC exterior joints. The rationality and accuracy of the new material are verified by comparing the simulation results with the test results.
Multi-Cohort Transcriptomic Profiling of Medical Gas Plasma-Treated Cancers Reveals the Role of Immunogenic Cell Death
Authors: Gkantaras Antonios , Kotzamanidis Charalampos , Kyriakidis Konstantinos , Farmaki Evangelia , Makedou Kali , Tzimagiorgis Georgios , Bekeschus Sander , Malousi Andigoni
Published: June 2024

The therapeutic potential of cold physical gas plasma operated at atmospheric pressure in oncology has been thoroughly demonstrated in numerous preclinical studies. The cytotoxic effect on malignant cells has been attributed mainly to biologically active plasma-generated compounds, namely, reactive oxygen and nitrogen species. The intracellular accumulation of reactive oxygen and nitrogen species interferes strongly with the antioxidant defense system of malignant cells, activating multiple signaling cascades and inevitably leading to oxidative stress-induced cell death. This study aims to determine whether plasma-induced cancer cell death operates through a universal molecular mechanism that is independent of the cancer cell type. Using whole transcriptome data, we sought to investigate the activation mechanism of plasma-treated samples in patient-derived prostate cell cultures, melanoma, breast, lymphoma, and lung cancer cells. The results from the standardized single-cohort gene expression analysis and parallel multi-cohort meta-analysis strongly indicate that plasma treatment globally induces cancer cell death through immune-mediated mechanisms, such as interleukin signaling, Toll-like receptor cascades, and MyD88 activation leading to pro-inflammatory cytokine release and tumor antigen presentation.
Metastatic Colorectal Cancer 2.0
Authors: Passardi Alessandro , Marisi Giorgia , Ulivi Paola
Published: June 2024

Colorectal cancer (CRC) ranks third in frequency among cancers diagnosed in males and second in females [...]
Outcome of Patients with Locally Advanced Rectal Cancer Pursuing Non-Surgical Strategy in National Cancer Database
Authors: Kakish Hanna , Ahmed Fasih A. , Ocuin Lee M. , Miller-Ocuin Jennifer L. , Steinhagen Emily , Hoehn Richard S. , Mahipal Amit , Towe Christopher W. , Chakrabarti Sakti
Published: June 2024

Background: Survival data on patients with locally advanced rectal cancer (LARC) undergoing non-operative management (NOM) in a real-world setting are lacking. Methods: We analyzed LARC patients from the National Cancer Database with the following features: treated between 2010 and 2020, age 18–65 years, Charlson comorbidity index (CCI) ≤ 1, received neoadjuvant multiagent chemotherapy plus radiation ≥ 45 Gray, and underwent surgery or NOM. Patients were stratified into two groups: (A) clinical T1-3 tumors with positive nodes (cT1-3N+) and (B) clinical T4 tumors, N+/− (cT4N+/−). We performed a comparative analysis of overall survival (OS) with NOM versus surgery by the Kaplan–Meier method and propensity score matching. Additionally, a multivariable analysis explored the association between NOM and OS. Results: NOM exhibited significantly lower OS than surgery in both groups. In cT1-3N+ patients, NOM resulted in a 5-year OS of 73.9% (95% confidence interval [CI] = 69.7–77.6%) versus 84.5% (95% CI = 83.6–85.3%) with surgery (p < 0.001). In the cT4N+/− group, NOM yielded a 5-year OS of 44.5% (95% CI = 37.0–51.8%) versus 72.5% (95% CI = 69.9–74.8%) with surgery (p < 0.001). Propensity score matching and multivariable analyses revealed similar conclusions. Conclusion: Patients with LARC undergoing NOM versus surgery in real-world settings appear to have inferior survival.
Survival in Elderly Ovarian Cancer Remains Challenging in the Nordic Countries
Authors: Hemminki Kari , Zitricky Frantisek , Försti Asta , Hemminki Akseli
Published: June 2024

Background: Despite treatment having improved through intensive surgical procedures and chemotherapy—and more recently, targeted therapies—ovarian cancer is the most fatal female cancer. As such, we wanted to analyze age-specific survival trends for ovarian cancer in Denmark, Finland, Norway and Sweden over the past 50 years, with a special aim of comparing survival development between the age groups. Methods: We modelled survival data from the NORDCAN database for 1-, 5- and conditional 5/1-year relative (between years 1 and 5) survival for ovarian cancer from 1972 to 2021. Results: Young patients had a 70% 5-year survival while the survival was only 30% for the oldest patients. Conditional survival showed that survival between years 1 and 5 did not improve for patients older than 60 years throughout the 50-year period, during which time the gaps between the youngest and the oldest patients widened. Conclusions: Improvement in 1-year survival was so large that it masked the modest development between years 1 and 5, resulting in a widening age disparity in 5-year survival. The current treatment practices, which appear increasingly effective for younger patients, have not helped remedy the large age differences in ovarian cancer survival. Early detection methods and therapeutic innovations are urgently needed, and aged patients need a special focus.
Anatolia: A Hotspot of Avian Genetic Diversity in the Western Palaearctic
Authors: Albayrak Tamer , Tunçel Tuğba , Öğe Pınar , Tietze Dieter Thomas , Forcina Giovanni
Published: June 2024

Located at the crossroads of two continents and at the southeastern edge of the Mediterranean Basin, Anatolia was one of the most important Pleistocene glacial refugia in the Western Palaearctic. As part of the Irano-Anatolian, Caucasus and Mediterranean Basin biodiversity hotspots, this region is also home to a rich avian community including nearly 400 breeding species. Nevertheless, research addressing the genetic structure and diversity of local bird populations is limited, and information on glacial refugia in this region is still scant, especially when compared to other large Mediterranean peninsulas, namely the Balkan, Italian and Iberian ones. In this study, we contribute to filling this gap by addressing the biogeographic pattern of four common resident songbirds—the Eurasian blue tit (Cyanistes caeruleus), the great tit (Parus major), the Eurasian chaffinch (Fringilla coelebs) and the Eurasian blackbird (Turdus merula)—and one endemic species—the Krüper’s nuthatch (Sitta krueperi)—by amplifying two mitochondrial DNA genes in individuals from Anatolia (n = 329) and comparing their sequences to those of conspecifics from the rest of their distribution range across the western Palaearctic (n = 357) deposited in public databases. The overall genetic structure of these species is consistent with a scenario of isolation for multiple populations in different refugia across Anatolia and subsequent secondary contact in the wake of ice retreat, which makes this region a hotspot of genetic diversity for both widespread and endemic avian species.
Enhancing Power Supply Flexibility in Renewable Energy Systems with Optimized Energy Dispatch in Coupled CHP, Heat Pump, and Thermal Storage
Authors: Chen Dongwen , Chu Zheng
Published: June 2024

The use of renewable energy by converting it into heat is an important form of storing energy in a usable form and improving the energy supply flexibility; therefore, the electricity–heating system (EHS) can cope with load fluctuations. However, relevant research is lacking on improving the energy supply limitations by the optimal dispatch of energy flow at the typical EHS, such as the coupled CHP–heat pump–thermal storage system (CCHTS). Based on the study of the energy supply characteristics of the CCHTS for extending the energy supply limitation, this study develops an optimal dispatch method using a heat pump (HP) and the thermal storage (TS) of heating networks to improve the flexibility of the CCHTS and the accommodation capacity of renewable energy. The maximum and minimum energy supply limitation model of the CCHTS and the output power characteristic model are established. Based on the piecewise power supply constraint, the energy flow of the EHS is optimized by using the quadratic programming algorithm. The CCHTS can significantly improve the energy supply flexibility; both coupled combined heat and power (CHP) + HP and coupled CHP + TS can improve the power supply flexibility, but the enhanced effect of CHP + HP is better than that of CHP + TS. An increase of 7.6% in wind power consumption is achieved. The consumption of renewable energy increases by 17.9% in the energy flow optimization results.
Alien vs. Native—Influence of Fallow Deer (Dama dama) Introduction on the Native Roe Deer (Capreolus capreolus) Population
Authors: Gryz Jakub , Krauze-Gryz Dagny , Jasińska Karolina D.
Published: June 2024

Fallow deer is one of the most widespread alien mammals in Europe. We documented the response of the roe deer population shortly after the fallow deer was introduced to a hunting ground in central Poland. Mean roe density dropped from 17.6 ind./100 ha to 10.5 ind./100 ha after the alien species was introduced. In the reference area, where fallow deer was absent, the roe deer density did not change in the analogue study period. At both study sites, mean roe deer productivity before fallow deer introduction was similar (1.6 juv./female). However, in the first study area, the productivity dropped to 1.4, while in the reference study area, it slightly increased to 1.75. The presence of fallow deer influenced roe deer space use negatively, i.e., the number of pellet groups of roe deer decreased with an increase in the number of fallow deer feces. Overall, the introduction of the fallow deer was successful and the population grew quickly. Yet, the economic impact of its introduction was far from satisfactory. At the same time, its negative influence on the roe deer was apparent. This shows that the fallow deer is an alien species threatening local biodiversity.
Temporal Recall Strategies in Rheumatoid Arthritis Patients’ Emotionally Intense Life Events
Authors: Balikó Fanni , Csókási Krisztina , Pohárnok Melinda , Vincze Orsolya , Kumánovics Gábor , Deme Marcell , Long Preston Alexander , Stamm Tanja
Published: June 2024

Background: Rheumatoid arthritis (RA) patients often encounter psychological challenges due to chronic pain, fatigue, side effects of medications, and disability. This study examines the relationship between autobiographical narratives and recollection patterns in RA patients. We investigated how different recall strategies for positive life events affect the emotional processing of negative episodes. We hypothesized that vividly recalling positive life events provides psychological resources that support a more intense emotional elaboration of stressful memories, allowing individuals to delve deeper into negative life experiences. Additionally, we explored the impact of these perspectives on self-reported well-being and physical health, proposing that re-living positive events improves overall well-being. Methods: We collected and analyzed high-point and low-point life-story episodes from 60 RA patients (85% female; age mean 61 ± 11 years; range 37–79) using episodic narrative interviews and the Narrative Categorical Content Analysis algorithm (NarrCat). Participants were categorized into 2 clusters based on their temporal perspective during high-point episodes: 25 used a Retrospective viewpoint, while 35 employed a Re-experiencing strategy. Depression and anxiety were assessed with the Hospital Anxiety and Depression Scale (HADS), and functioning was measured using the Health Assessment Questionnaire (HAQ). Results: The Re-experiencing group, which was more likely to articulate their high-point episode in vivid and real-time narrative, used more psychological perspectives (U(58) = 223, p < 0.01) and showed heightened emotional frequency (U(58) = 280, p < 0.05; positive: U(58) = 328, p < 0.05; negative: U(58) = 278, p < 0.05) in low-point episodes. No significant difference emerged between the two groups regarding psychological state (anxiety, depressive symptoms) and physical impairment. Conclusions: Vividly recalling positive events may facilitate a deeper exploration of negative memories. The Re-experiencing group showed increased positive emotions during low points, suggesting better emotion regulation. However, no significant association was found between recalling strategies, psychological state, and physical impairment. This indicates that further research is needed to determine whether re-experiencing positive life events is adaptive or maladaptive.
Glyphosate-Induced Phosphonatase Operons in Soil Bacteria of the Genus Achromobacter
Authors: Epiktetov Dmitry O. , Sviridov Alexey V. , Tarlachkov Sergey V. , Shushkova Tatyana V. , Toropygin Ilya Yu. , Leontievsky Alexey A.
Published: June 2024

Achromobacter insolitus and Achromobacter aegrifaciens, bacterial degraders of the herbicide glyphosate, were found to induce phosphonatase (phosphonoacetaldehyde hydrolase, EC 3.11.1.1) when grown on minimal media with glyphosate as the sole source of phosphorus. The phosphonatases of the strains were purified to an electrophoretically homogeneous state and characterized. The enzymes differed in their kinetic characteristics and some other parameters from the previously described phosphonatases. The phosphonatase of A. insolitus was first revealed to separate into two stable forms, which had similar kinetic characteristics but interacted differently with affinity and ion-exchange resins. The genomes of the investigated bacteria were sequenced. The phosphonatase genes were identified, and their context was determined: the bacteria were shown to have gene clusters, which, besides the phosphonatase operon, included genes for LysR-type transcription activator (substrate sensor) and putative iron-containing oxygenase PhnHD homologous to monooxygenases PhnY and TmpB of marine organophosphonate degraders. Genes of 2-aminoethylphosphonate aminotransferase (PhnW, EC 2.6.1.37) were absent in the achromobacterial phosphonatase operons; instead, we revealed the presence of genes encoding the putative flavin oxidase HpnW. In silico simulation showed 1-hydroxy-2-aminoethylphosphonate to be the most likely substrate of the new monooxygenase, and a number of glycine derivatives structurally similar to glyphosate to be substrates of flavin oxidase.
A Systematic Review of Genetics- and Molecular-Pathway-Based Machine Learning Models for Neurological Disorder Diagnosis
Authors: Aljarallah Nasser Ali , Dutta Ashit Kumar , Sait Abdul Rahaman Wahab
Published: June 2024

The process of identification and management of neurological disorder conditions faces challenges, prompting the investigation of novel methods in order to improve diagnostic accuracy. In this study, we conducted a systematic literature review to identify the significance of genetics- and molecular-pathway-based machine learning (ML) models in treating neurological disorder conditions. According to the study’s objectives, search strategies were developed to extract the research studies using digital libraries. We followed rigorous study selection criteria. A total of 24 studies met the inclusion criteria and were included in the review. We classified the studies based on neurological disorders. The included studies highlighted multiple methodologies and exceptional results in treating neurological disorders. The study findings underscore the potential of the existing models, presenting personalized interventions based on the individual’s conditions. The findings offer better-performing approaches that handle genetics and molecular data to generate effective outcomes. Moreover, we discuss the future research directions and challenges, emphasizing the demand for generalizing existing models in real-world clinical settings. This study contributes to advancing knowledge in the field of diagnosis and management of neurological disorders.
Mechanisms Underlying the Effects of Chloroquine on Red Blood Cells Metabolism
Authors: Russo Annamaria , Patanè Giuseppe Tancredi , Putaggio Stefano , Lombardo Giovanni Enrico , Ficarra Silvana , Barreca Davide , Giunta Elena , Tellone Ester , Laganà Giuseppina
Published: June 2024

Chloroquine (CQ) is a 4-aminoquinoline derivative largely employed in the management of malaria. CQ treatment exploits the drug’s ability to cross the erythrocyte membrane, inhibiting heme polymerase in malarial trophozoites. Accumulation of CQ prevents the conversion of heme to hemozoin, causing its toxic buildup, thus blocking the survival of Plasmodium parasites. Recently, it has been reported that CQ is able to exert antiviral properties, mainly against HIV and SARS-CoV-2. This renewed interest in CQ treatment has led to the development of new studies which aim to explore its side effects and long-term outcome. Our study focuses on the effects of CQ in non-parasitized red blood cells (RBCs), investigating hemoglobin (Hb) functionality, the anion exchanger 1 (AE1) or band 3 protein, caspase 3 and protein tyrosine phosphatase 1B (PTP-1B) activity, intra and extracellular ATP levels, and the oxidative state of RBCs. Interestingly, CQ influences the functionality of both Hb and AE1, the main RBC proteins, affecting the properties of Hb oxygen affinity by shifting the conformational structure of the molecule towards the R state. The influence of CQ on AE1 flux leads to a rate variation of anion exchange, which begins at a concentration of 2.5 μM and reaches its maximum effect at 20 µM. Moreover, a significant decrease in intra and extracellular ATP levels was observed in RBCs pre-treated with 10 µM CQ vs. erythrocytes under normal conditions. This effect is related to the PTP-1B activity which is reduced in RBCs incubated with CQ. Despite these metabolic alterations to RBCs caused by exposure to CQ, no signs of variations in oxidative state or caspase 3 activation were recorded. Our results highlight the antithetical effects of CQ on the functionality and metabolism of RBCs, and encourage the development of new research to better understand the multiple potentiality of the drug.
MicroRNA Expression in Patients with Coronary Artery Disease and Hypertension—A Systematic Review
Authors: Kondracki Bartosz , Kłoda Mateusz , Jusiak-Kłoda Anna , Kondracka Adrianna , Waciński Jakub , Waciński Piotr
Published: June 2024

Coronary artery disease (CAD) and hypertension significantly contribute to cardiovascular morbidity and mortality. MicroRNAs (miRNAs) have recently emerged as promising biomarkers and therapeutic targets for these conditions. This systematic review conducts a thorough analysis of the literature, with a specific focus on investigating miRNA expression patterns in patients with CAD and hypertension. This review encompasses an unspecified number of eligible studies that employed a variety of patient demographics and research methodologies, resulting in diverse miRNA expression profiles. This review highlights the complex involvement of miRNAs in CAD and hypertension and the potential for advances in diagnostic and therapeutic strategies. Future research endeavors are imperative to validate these findings and elucidate the precise roles of miRNAs in disease progression, offering promising avenues for innovative diagnostic tools and targeted interventions.
Integrated miRNA–mRNA Analysis Reveals Critical miRNAs and Targets in Diet-Induced Obesity-Related Glomerulopathy
Authors: López-Martínez Marina , Armengol Maria Pilar , Pey Irina , Farré Xavier , Rodríguez-Martínez Paula , Ferrer Mireia , Porrini Esteban , Luis-Lima Sergio , Díaz-Martín Laura , Rodríguez-Rodríguez Ana Elena , Cruz-Perera Coriolano , Alcalde Marta , Navarro-Díaz Maruja
Published: June 2024

This study aimed to investigate obesity-related glomerulopathy (ORG) at cellular, structural, and transcriptomic levels. Thirty Wistar rats were randomized into two groups: 15 rats were fed with a standard diet (SD-rats), and 15 rats were fed with a high-fat diet (HFD-rats). After 10 weeks, the weight, kidney function, histological features, and transcriptomic changes were assessed. HFD-rats gained significantly more weight (55.8% vs. 29.2%; p < 0.001) and albuminuria (10,384.04 ng/mL vs. 5845.45 ng/mL; p < 0.001) compared to SD-rats. HFD-rats exhibited early stages of ORG, with predominant mesangial matrix increase and podocyte hypertrophy (PH). These lesions correlated with differentially expressed (DE) genes and miRNAs. Functional analysis showed that miR-205, which was DE in both the kidneys and urine of HFD-rats, negatively regulated the PTEN gene, promoting lipid endocytosis in podocytes. The downregulation of PTEN was proved through a higher PTEN/nephrin ratio in the SD-rats and the presence of lipid vacuoles in HFD-podocytes. This study has found a specific targetome of miRNAs and gene expression in early stages of ORG. Also, it emphasizes the potential value of miR-205 as a urinary biomarker for detecting podocyte injury in ORG, offering a tool for early diagnosis, and opening new avenues for future therapeutic research of obesity-related glomerulopathy.
The Uniform Distribution of Hydroxyapatite in a Polyurethane Foam-Based Scaffold (PU/HAp) to Enhance Bone Repair in a Calvarial Defect Model
Authors: Chen Chiu-Fang , Chou Ya-Shuan , Lee Tzer-Min , Fu Yin-Chih , Ou Shih-Fu , Chen Szu-Hsien , Lee Tien-Ching , Wang Yan-Hsiung
Published: June 2024

Polyurethane (PU) is a promising material for addressing challenges in bone grafting. This study was designed to enhance the bone grafting capabilities of PU by integrating hydroxyapatite (HAp), which is known for its osteoconductive and osteoinductive potential. Moreover, a uniform distribution of HAp in the porous structure of PU increased the effectiveness of bone grafts. PEG/APTES-modified scaffolds were prepared through self-foaming reactions. A uniform pore structure was generated during the spontaneous foaming reaction, and HAp was uniformly distributed in the PU structure (PU15HAp and PU30HAp) during foaming. Compared with the PU scaffolds, the HAp-modified PU scaffolds exhibited significantly greater protein absorption. Importantly, the effect of the HAp-modified PU scaffold on bone repair was tested in a rat calvarial defect model. The microstructure of the newly formed bone was analyzed with microcomputed tomography (μ-CT). Bone regeneration at the defect site was significantly greater in the HAp-modified PU scaffold group than in the PU group. This innovative HAp-modified PU scaffold improves current bone graft materials, providing a promising avenue for improved bone regeneration.
Mast Cells in Human Health and Diseases 2.0
Authors: Traina Giovanna
Published: June 2024

This Special Issue collects some scientific pieces of the multifaceted research on the mast cell (MC), and it intends to highlight the broad spectrum of activity that MCs have, both in physiological conditions and in pathological states, focusing attention on some of them [...]
Analysis of the BDNF Gene rs6265 Polymorphism in a Group of Women with Alcohol Use Disorder, Taking into Account Personality Traits
Authors: Boroń Agnieszka , Suchanecka Aleksandra , Chmielowiec Krzysztof , Chmielowiec Jolanta , Masiak Jolanta , Trybek Grzegorz , Strońska-Pluta Aleksandra , Rychel Monika , Grzywacz Anna
Published: June 2024

It seems that BDNF has a direct influence on the brain pathways and is typically engaged during the processing of rewards. A surge in BDNF levels in the ventral tegmental area (the region from which the dopaminergic neurons of the mesocorticolimbic dopamine system originate and extend to the dorsolateral and ventromedial striatum) triggers a state of reward similar to that produced by opiates in animal studies. The aims of the study were (1) to analyze the association of the BDNF gene rs6265 polymorphism with AUD (alcohol use disorder) in women, (2) analyze personality and anxiety in alcohol-dependent and control woman, and (3) conduct an interaction analysis of rs6265 on personality, anxiety, and alcohol dependence. Our study found a notable interaction between the anxiety (trait and state), neuroticism, rs6265, and AUD. The alcohol AUD G/A genotype carriers revealed higher level of the anxiety trait (p < 0.0001) and neuroticism (p < 0.0001) compared to the control group with G/A and G/G genotypes. The alcohol use disorder subjects with the G/A genotype displayed higher levels of an anxiety state than the control group with G/A (p < 0.0001) and G/G (p = 0.0014) genotypes. Additionally, the alcohol use disorder subjects with the G/G genotype obtained lower levels of agreeability compared to the controls with G/A (p < 0.0001) and G/G (p < 0.0001) genotypes. Our study indicates that anxiety (trait and state) and neuroticism are interacting with the BDNF gene rs6265 polymorphism in alcohol-dependent women. Characteristics like anxiety (both as a trait and a state) and neuroticism could have a significant impact on the mechanism of substance dependency, particularly in females who are genetically susceptible. This is regardless of the reward system that is implicated in the emotional disruptions accompanying anxiety and depression.
Efficient DNA Coding Algorithm for Polymerase Chain Reaction Amplification Information Retrieval
Authors: Wang Qing , Zhang Shufang , Li Yuhui
Published: June 2024

Polymerase Chain Reaction (PCR) amplification is widely used for retrieving information from DNA storage. During the PCR amplification process, nonspecific pairing between the 3’ end of the primer and the DNA sequence can cause cross-talk in the amplification reaction, leading to the generation of interfering sequences and reduced amplification accuracy. To address this issue, we propose an efficient coding algorithm for PCR amplification information retrieval (ECA-PCRAIR). This algorithm employs variable-length scanning and pruning optimization to construct a codebook that maximizes storage density while satisfying traditional biological constraints. Subsequently, a codeword search tree is constructed based on the primer library to optimize the codebook, and a variable-length interleaver is used for constraint detection and correction, thereby minimizing the likelihood of nonspecific pairing. Experimental results demonstrate that ECA-PCRAIR can reduce the probability of nonspecific pairing between the 3’ end of the primer and the DNA sequence to 2–25%, enhancing the robustness of the DNA sequences. Additionally, ECA-PCRAIR achieves a storage density of 2.14–3.67 bits per nucleotide (bits/nt), significantly improving storage capacity.