bluuebunny/crossref_metadata_2025 huggingface dataset:
DOES INFLATION TARGETING MATTER? AN EXPERIMENTAL INVESTIGATION
Authors: Cornand Camille , M'baye Cheick Kader
Published: August 2016

We use laboratory experiments with human subjects to test the relevance of different inflation-targeting regimes. In particular and within the standard New Keynesian model, we evaluate to what extent communication of the inflation target is relevant to the success of inflation targeting. We find that if the central bank cares only about inflation stabilization, announcing the inflation target does not make a difference in terms of macroeconomic performance compared with a standard active monetary policy. However, if the central bank also cares about the stabilization of economic activity, communicating the target helps to reduce the volatility of inflation, interest rate, and output gap, although their average levels are not affected. This finding is consistent with the theoretical literature and provides a rationale for the adoption of a flexible inflation-targeting regime.
Soft drinks consumption and child behaviour problems: the role of food insecurity and sleep patterns
Authors: King Christian
Published: August 2016

Objective

To examine whether the association between soft drinks consumption and child behaviour problems differs by food security status and sleep patterns in young children.

Design

Cross-sectional observational data from the Fragile Families and Child Wellbeing Study (FFCWS), which collected information on food insecurity, soft drinks consumption, sleep patterns and child behaviour problems. Bivariate and multivariate ordinary least-squares regression analyses predicting child behaviour problems and accounting for socio-economic factors and household characteristics were performed.

Setting

Twenty urban cities in the USA with a population of 200 000 or more.

Subjects

Parental interviews of 2829 children who were about 5 years old.

Results

Soft drinks consumption was associated with aggressive behaviours, withdrawn and attention problems for children aged 5 years. However, the association differed by food security status. The association was mostly statistically insignificant among food-secure children after accounting for socio-economic and demographic characteristics. On the other hand, soft drinks consumption was associated with behaviour problems for food-insecure children even after accounting for these factors. However, after accounting for child sleep patterns, the association between soft drinks consumption and child behaviour problems became statistically insignificant for food-insecure children.

Conclusions

The negative association between soft drinks consumption and child behaviour problems could be explained by sleep problems for food-insecure children. Since about 21 % of households with children are food insecure, targeted efforts to reduce food insecurity would help improve dietary (reduce soft drinks consumption) and health behaviours (improve sleep) and reduce child behaviour problems.
Discretionary food and beverage consumption and its association with demographic characteristics, weight status, and fruit and vegetable intakes in Australian adults
Authors: Sui Zhixian , Wong Weng Kei , Louie Jimmy Chun Yu , Rangan Anna
Published: August 2016

Objective

Excessive consumption of discretionary foods/beverages in the Australian population has been identified, increasing the risk of obesity and chronic disease. The present study aimed to examine the associations between demographic, anthropometric and dietary factors and the consumption of discretionary foods, discretionary beverages and discretionary foods/beverages combined.

Design

Discretionary food/beverage consumption reported in two 24 h recalls was analysed, stratified by gender, age, socio-economic status, country of birth, BMI, waist circumference, and fruit and vegetable intakes.

Setting

2011–12 National Nutrition and Physical Activity Survey.

Subjects

Australian adults (n 7873) aged 19 years or above.

Results

Mean discretionary food and beverage consumption was 631 g (28 % by weight from foods; 72 % from beverages), providing 2721 kJ of energy intake (72 % from foods; 28 % from beverages). Total discretionary food/beverage consumption was higher in younger age groups (P<0·001), those in lower socio-economic categories (P<0·001), those born in Australia (P<0·001), those with higher BMI (P<0·001) and those with lower fruit (P<0·001) or vegetable intake (P<0·001). Discretionary beverage consumption (β=6·6, P<0·001) was more strongly associated with BMI than discretionary food consumption (β=0·5, P=0·01).

Conclusions

Total discretionary food/beverage consumption as well as discretionary foods alone and discretionary beverages alone were associated with BMI in Australian adults. In addition, high intakes were associated with younger age, lower socio-economic status, and lower consumption of fruit and vegetables.
The longevity of habitable planets and the development of intelligent life
Authors: Simpson Fergus
Published: August 2016

Why did the emergence of our species require a timescale similar to the entire habitable period of our planet? Our late appearance has previously been interpreted by Carter (2008) as evidence that observers typically require a very long development time, implying that intelligent life is a rare occurrence. Here we present an alternative explanation, which simply asserts that many planets possess brief periods of habitability. We also propose that the rate-limiting step for the formation of observers is the enlargement of species from an initially microbial state. In this scenario, the development of intelligent life is a slow but almost inevitable process, greatly enhancing the prospects of future search for extra-terrestrial intelligence (SETI) experiments such as the Breakthrough Listen project.
Recognising Unpaid Care in Private Pension Schemes
Authors: Hamilton Myra , Thomson Cathy
Published: August 2016

Parents and carers often have interrupted workforce histories, causing gaps in their pension contributions and hence significantly lower retirement incomes. In some countries, to ameliorate these inequalities, carer credits have been introduced to maintain public pension contributions during periods of workforce absence. But improvements to credits in public schemes have taken place alongside a shift to private pensions that widens inequalities for carers. Introducing carer credits to private pensions is one method of addressing these inequalities. A search for examples of credits to private schemes in OECD countries revealed that, at present, they are rare and limited. This article sets out the design features and principles that should underpin carer credits to private pensions.
Lipids and leukocytes in newborn umbilical vein blood, birth weight and maternal body mass index
Authors: Brittos T. , de Souza W. B. , Anschau F. , Pellanda L.
Published: August 2016

Maternal obesity during pregnancy may influence fetal development and possibly predispose offspring to cardiovascular disease. The aim of the present study was to evaluate the relationship between maternal pre-pregnancy body mass index (BMI) and weight gain during pregnancy, and newborn birth weight, with lipid profile, high-sensitivity C-reactive protein (hs-CRP) and leukocyte in newborns. We performed a cross-sectional study of 245 mothers and their children. Blood was collected from the umbilical vein and assayed for lipid profile, hs-CRP and leukocyte count. Newborns average weight was 3241 g, total cholesterol 53.9 mg/dl, high-density lipoprotein cholesterol (HDL-c) 21.9 mg/dl, low-density lipoprotein cholesterol (LDL-c) 26.2 mg/dl, triglyceride 29.5 mg/dl and leukocytes 13,777/mm3. There was a direct correlation of pre-pregnancy BMI of overweight mothers with total cholesterol (r=0.220, P=0.037) and LDL-c (r=0.268, P=0.011) of newborns. Total cholesterol, LDL-c and HDL-c were higher in pre-term newborns (66.3±19.7, 35.9±14.6 and 25.2±7.7 mg/dl, respectively) that in full-term (52.4±13.1, 25.0±8.7 and 21.5±6.0 mg/dl), with P=0.001, 0.001 and 0.003, respectively. Leukocyte counts were higher in full-term newborns (14,268±3982/mm3) compared with pre-term (9792±2836/mm3, P<0.0001). There was a direct correlation between birth weight and leukocyte counts of newborns (r=0.282, P<0.0001). These results suggest the possible interaction of maternal weight and fetal growth with lipid metabolism and leukocyte count in the newborn, which may be linked to programming of the immune system.
Early inherited risk for anxiety moderates the association between fathers’ child-centered parenting and early social inhibition
Authors: Brooker R. J. , Alto K. M. , Marceau K. , Najjar R. , Leve L. D. , Ganiban J. M. , Shaw D. S. , Reiss D. , Neiderhiser J. M.
Published: August 2016

Studies of the role of the early environment in shaping children’s risk for anxiety problems have produced mixed results. It is possible that inconsistencies in previous findings result from a lack of consideration of a putative role for inherited influences moderators on the impact of early experiences. Early inherited influences not only contribute to vulnerabilities for anxiety problems throughout the lifespan, but can also modulate the ways that the early environment impacts child outcomes. In the current study, we tested the effects of child-centered parenting behaviors on putative anxiety risk in young children who differed in levels of inherited vulnerability. We tested this using a parent–offspring adoption design and a sample in which risk for anxiety problems and parenting behaviors were assessed in both mothers and fathers. Inherited influences on anxiety problems were assessed as anxiety symptoms in biological parents. Child-centered parenting was observed in adoptive mothers and fathers when children were 9 months old. Social inhibition, an early temperament marker of anxiety risk, was observed at child ages 9 and 18 months. Inherited influences on anxiety problems moderated the link between paternal child-centered parenting during infancy and social inhibition in toddlerhood. For children whose birth parents reported high levels of anxiety symptoms, greater child-centered parenting in adoptive fathers was related to greater social inhibition 9 months later. For children whose birth parents reported low levels of anxiety symptoms, greater child-centered parenting in adoptive fathers was related to less social inhibition across the same period.
Response inhibition deficits: Reliability of alcohol-related assessment tasks
Authors: Czapla Marta , Vollstädt-Klein Sabine , Fauth-Bühler Mira , Best Eva , Fix Maria , Mann Karl , Herpertz Sabine C. , Loeber Sabine
Published: August 2016

Abstract. Background: While models of addictive behaviour stress the role of an impairment of response inhibition when drug-dependent individuals are confronted with drug-associated stimuli, results from different studies are conflicting. However, there is a considerable lack of studies that assess the reliability of tasks to assess deficits of response inhibition when drug-associated stimuli are presented. Methods: In the present paper we present results from four different studies in which either a stop-signal task (study 1), a go/no-go task (study 2/3) or a go/no-go shifting task (study 4) with alcohol-related stimuli was administered to alcohol-dependent patients or control participants and split-half and/or test-retest reliability of the different outcome measures calculated. Results: Our results suggest that the go/no-go task and the go/no-go shifting task are more reliable tasks to assess impairment of inhibition in response to alcohol-associated stimuli than to the stop-signal task. Especially the go/no-go shifting task achieves at least acceptable split-half as well as test-retest reliability indices for outcome measure related to the presentation of alcohol-associated stimuli. Nevertheless, for alcohol-dependent patients reliability indices are generally considerably lower than for control participants and thus care should be taken when these tasks are administered to alcohol-dependent patients. Conclusion: Future studies are warranted to enhance our understanding of true effects and random error
Patienten- und Therapeutensicht auf Abbruchgründe bei Therapien nach § 64 StGB
Authors: Querengässer Jan , Bulla Jan , Mielke Reinhard , Hoffmann Klaus , Ross Thomas
Published: August 2016

Zusammenfassung. Einleitung: Hohe Erledigungsquoten wegen Aussichtslosigkeit einer Weiterbehandlung beschäftigen den Maßregelvollzug nach § 64 StGB seit Jahren. Einige behandlungsprognostisch relevante Patienteneigenschaften sind bekannt, für den Einzelfall sind sie aber schwierig zu bewerten. Die konkreten Gründe für die vorzeitige Beendigung einer Therapie wurden kaum untersucht. Dasselbe gilt für die subjektive Einschätzung eines Therapieabbruchs von Patienten- und Therapeutenseite. Zielsetzung: Ein Perspektivenvergleich von Patienten und Therapeuten in Bezug auf Erledigungsgründe einer Unterbringung nach § 64 StGB. Methodik: 37 Patienten, bei denen ein Therapieabbruch angeregt wurde, bewerteten potentielle Abbruchgründe. Bei 29 Patienten erfolgte dies auch durch die fallführenden Therapeuten. Ergebnisse: Die Patienten gaben mehr Abbruchgründe an als die Therapeuten. Aus Patientensicht dominierten die Wahrnehmung eigener Anspannung und Aggressivität, Auseinandersetzungen mit Mitpatienten sowie andere negative Einflüsse in einem schlechten Stationsklima. Die Therapeuten hoben vor allem die mangelnde Änderungsbereitschaft der Patienten hervor. Deutlich übereinstimmende Angaben zwischen Patienten und Therapeuten waren selten. Schlussfolgerung: Mit therapeutischen Inhalten in Beziehung stehende Denk- und Erlebensmuster von Patienten sollten regelmäßig erfasst, evaluiert und mit der Therapeutenperspektive abgeglichen werden. Zeigen sich deutliche Differenzen, so deutet dies auf Probleme in der Therapiebeziehung hin, die frühzeitig bearbeitet werden könnten.
Integrative analysis of mutational and transcriptional profiles reveals driver mutations of metastatic breast cancers
Authors: Lee Ji-Hyun , Zhao Xing-Ming , Yoon Ina , Lee Jin Young , Kwon Nam Hoon , Wang Yin-Ying , Lee Kyung-Min , Lee Min-Joo , Kim Jisun , Moon Hyeong-Gon , In Yongho , Hao Jin-Kao , Park Kyung-Mii , Noh Dong-Young , Han Wonshik , Kim Sunghoon
Published: August 2016

Despite the explosion in the numbers of cancer genomic studies, metastasis is still the major cause of cancer mortality. In breast cancer, approximately one-fifth of metastatic patients survive 5 years. Therefore, detecting the patients at a high risk of developing distant metastasis at first diagnosis is critical for effective treatment strategy. We hereby present a novel systems biology approach to identify driver mutations escalating the risk of metastasis based on both exome and RNA sequencing of our collected 78 normal-paired breast cancers. Unlike driver mutations occurring commonly in cancers as reported in the literature, the mutations detected here are relatively rare mutations occurring in less than half metastatic samples. By supposing that the driver mutations should affect the metastasis gene signatures, we develop a novel computational pipeline to identify the driver mutations that affect transcription factors regulating metastasis gene signatures. We identify driver mutations in ADPGK, NUP93, PCGF6, PKP2 and SLC22A5, which are verified to enhance cancer cell migration and prompt metastasis with in vitro experiments. The discovered somatic mutations may be helpful for identifying patients who are likely to develop distant metastasis.
Intertidal resource use over millennia enhances forest productivity
Authors: Trant Andrew J. , Nijland Wiebe , Hoffman Kira M. , Mathews Darcy L. , McLaren Duncan , Nelson Trisalyn A. , Starzomski Brian M.
Published: August 2016

Human occupation is usually associated with degraded landscapes but 13,000 years of repeated occupation by British Columbia’s coastal First Nations has had the opposite effect, enhancing temperate rainforest productivity. This is particularly the case over the last 6,000 years when intensified intertidal shellfish usage resulted in the accumulation of substantial shell middens. We show that soils at habitation sites are higher in calcium and phosphorous. Both of these are limiting factors in coastal temperate rainforests. Western redcedar (Thuja plicata) trees growing on the middens were found to be taller, have higher wood calcium, greater radial growth and exhibit less top die-back. Coastal British Columbia is the first known example of long-term intertidal resource use enhancing forest productivity and we expect this pattern to occur at archaeological sites along coastlines globally.
Rewiring of jasmonate and phytochrome B signalling uncouples plant growth-defense tradeoffs
Authors: Campos Marcelo L. , Yoshida Yuki , Major Ian T. , de Oliveira Ferreira Dalton , Weraduwage Sarathi M. , Froehlich John E. , Johnson Brendan F. , Kramer David M. , Jander Georg , Sharkey Thomas D. , Howe Gregg A.
Published: August 2016

Plants resist infection and herbivory with innate immune responses that are often associated with reduced growth. Despite the importance of growth-defense tradeoffs in shaping plant productivity in natural and agricultural ecosystems, the molecular mechanisms that link growth and immunity are poorly understood. Here, we demonstrate that growth-defense tradeoffs mediated by the hormone jasmonate are uncoupled in an Arabidopsis mutant (jazQ phyB) lacking a quintet of Jasmonate ZIM-domain transcriptional repressors and the photoreceptor phyB. Analysis of epistatic interactions between jazQ and phyB reveal that growth inhibition associated with enhanced anti-insect resistance is likely not caused by diversion of photoassimilates from growth to defense but rather by a conserved transcriptional network that is hardwired to attenuate growth upon activation of jasmonate signalling. The ability to unlock growth-defense tradeoffs through relief of transcription repression provides an approach to assemble functional plant traits in new and potentially useful ways.
Synthesis and characterization of attosecond light vortices in the extreme ultraviolet
Authors: Géneaux R. , Camper A. , Auguste T. , Gobert O. , Caillat J. , Taïeb R. , Ruchon T.
Published: August 2016

Infrared and visible light beams carrying orbital angular momentum (OAM) are currently thoroughly studied for their extremely broad applicative prospects, among which are quantum information, micromachining and diagnostic tools. Here we extend these prospects, presenting a comprehensive study for the synthesis and full characterization of optical vortices carrying OAM in the extreme ultraviolet (XUV) domain. We confirm the upconversion rules of a femtosecond infrared helically phased beam into its high-order harmonics, showing that each harmonic order carries the total number of OAM units absorbed in the process up to very high orders (57). This allows us to synthesize and characterize helically shaped XUV trains of attosecond pulses. To demonstrate a typical use of these new XUV light beams, we show our ability to generate and control, through photoionization, attosecond electron beams carrying OAM. These breakthroughs pave the route for the study of a series of fundamental phenomena and the development of new ultrafast diagnosis tools using either photonic or electronic vortices.
A stochastic model of randomly accelerated walkers for human mobility
Authors: Gallotti Riccardo , Bazzani Armando , Rambaldi Sandro , Barthelemy Marc
Published: August 2016

Recent studies of human mobility largely focus on displacements patterns and power law fits of empirical long-tailed distributions of distances are usually associated to scale-free superdiffusive random walks called Lévy flights. However, drawing conclusions about a complex system from a fit, without any further knowledge of the underlying dynamics, might lead to erroneous interpretations. Here we show, on the basis of a data set describing the trajectories of 780,000 private vehicles in Italy, that the Lévy flight model cannot explain the behaviour of travel times and speeds. We therefore introduce a class of accelerated random walks, validated by empirical observations, where the velocity changes due to acceleration kicks at random times. Combining this mechanism with an exponentially decaying distribution of travel times leads to a short-tailed distribution of distances which could indeed be mistaken with a truncated power law. These results illustrate the limits of purely descriptive models and provide a mechanistic view of mobility.
Cross-species identification of genomic drivers of squamous cell carcinoma development across preneoplastic intermediates
Authors: Chitsazzadeh Vida , Coarfa Cristian , Drummond Jennifer A. , Nguyen Tri , Joseph Aaron , Chilukuri Suneel , Charpiot Elizabeth , Adelmann Charles H. , Ching Grace , Nguyen Tran N. , Nicholas Courtney , Thomas Valencia D. , Migden Michael , MacFarlane Deborah , Thompson Erika , Shen Jianjun , Takata Yoko , McNiece Kayla , Polansky Maxim A. , Abbas Hussein A. , Rajapakshe Kimal , Gower Adam , Spira Avrum , Covington Kyle R. , Xiao Weimin , Gunaratne Preethi , Pickering Curtis , Frederick Mitchell , Myers Jeffrey N. , Shen Li , Yao Hui , Su Xiaoping , Rapini Ronald P. , Wheeler David A. , Hawk Ernest T. , Flores Elsa R. , Tsai Kenneth Y.
Published: August 2016

Cutaneous squamous cell carcinoma (cuSCC) comprises 15–20% of all skin cancers, accounting for over 700,000 cases in USA annually. Most cuSCC arise in association with a distinct precancerous lesion, the actinic keratosis (AK). To identify potential targets for molecularly targeted chemoprevention, here we perform integrated cross-species genomic analysis of cuSCC development through the preneoplastic AK stage using matched human samples and a solar ultraviolet radiation-driven Hairless mouse model. We identify the major transcriptional drivers of this progression sequence, showing that the key genomic changes in cuSCC development occur in the normal skin to AK transition. Our data validate the use of this ultraviolet radiation-driven mouse cuSCC model for cross-species analysis and demonstrate that cuSCC bears deep molecular similarities to multiple carcinogen-driven SCCs from diverse sites, suggesting that cuSCC may serve as an effective, accessible model for multiple SCC types and that common treatment and prevention strategies may be feasible.
Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity
Authors: Li Chia-Wei , Lim Seung-Oe , Xia Weiya , Lee Heng-Huan , Chan Li-Chuan , Kuo Chu-Wei , Khoo Kay-Hooi , Chang Shih-Shin , Cha Jong-Ho , Kim Taewan , Hsu Jennifer L. , Wu Yun , Hsu Jung-Mao , Yamaguchi Hirohito , Ding Qingqing , Wang Yan , Yao Jun , Lee Cheng-Chung , Wu Hsing-Ju , Sahin Aysegul A. , Allison James P. , Yu Dihua , Hortobagyi Gabriel N. , Hung Mien-Chie
Published: August 2016

Extracellular interaction between programmed death ligand-1 (PD-L1) and programmed cell death protein-1 (PD-1) leads to tumour-associated immune escape. Here we show that the immunosuppression activity of PD-L1 is stringently modulated by ubiquitination and N-glycosylation. We show that glycogen synthase kinase 3β (GSK3β) interacts with PD-L1 and induces phosphorylation-dependent proteasome degradation of PD-L1 by β-TrCP. In-depth analysis of PD-L1 N192, N200 and N219 glycosylation suggests that glycosylation antagonizes GSK3β binding. In this regard, only non-glycosylated PD-L1 forms a complex with GSK3β and β-TrCP. We also demonstrate that epidermal growth factor (EGF) stabilizes PD-L1 via GSK3β inactivation in basal-like breast cancer. Inhibition of EGF signalling by gefitinib destabilizes PD-L1, enhances antitumour T-cell immunity and therapeutic efficacy of PD-1 blockade in syngeneic mouse models. Together, our results link ubiquitination and glycosylation pathways to the stringent regulation of PD-L1, which could lead to potential therapeutic strategies to enhance cancer immune therapy efficacy.
Optical patterning of trapped charge in nitrogen-doped diamond
Authors: Jayakumar Harishankar , Henshaw Jacob , Dhomkar Siddharth , Pagliero Daniela , Laraoui Abdelghani , Manson Neil B. , Albu Remus , Doherty Marcus W. , Meriles Carlos A.
Published: August 2016

The nitrogen-vacancy (NV) centre in diamond is emerging as a promising platform for solid-state quantum information processing and nanoscale metrology. Of interest in these applications is the manipulation of the NV charge, which can be attained by optical excitation. Here, we use two-colour optical microscopy to investigate the dynamics of NV photo-ionization, charge diffusion and trapping in type-1b diamond. We combine fixed-point laser excitation and scanning fluorescence imaging to locally alter the concentration of negatively charged NVs, and to subsequently probe the corresponding redistribution of charge. We uncover the formation of spatial patterns of trapped charge, which we qualitatively reproduce via a model of the interplay between photo-excited carriers and atomic defects. Further, by using the NV as a probe, we map the relative fraction of positively charged nitrogen on localized optical excitation. These observations may prove important to transporting quantum information between NVs or to developing three-dimensional, charge-based memories.
A social-ecological database to advance research on infrastructure development impacts in the Brazilian Amazon
Authors: Tucker Lima Joanna M. , Valle Denis , Moretto Evandro Mateus , Pulice Sergio Mantovani Paiva , Zuca Nadia Lucia , Roquetti Daniel Rondinelli , Beduschi Liviam Elizabeth Cordeiro , Praia Amanda Salles , Okamoto Claudia Parucce Franco , da Silva Carvalhaes Vinicius Leite , Branco Evandro Albiach , Barbezani Bruna , Labandera Emily , Timpe Kelsie , Kaplan David
Published: August 2016

Recognized as one of the world’s most vital natural and cultural resources, the Amazon faces a wide variety of threats from natural resource and infrastructure development. Within this context, rigorous scientific study of the region’s complex social-ecological system is critical to inform and direct decision-making toward more sustainable environmental and social outcomes. Given the Amazon’s tightly linked social and ecological components and the scope of potential development impacts, effective study of this system requires an easily accessible resource that provides a broad and reliable data baseline. This paper brings together multiple datasets from diverse disciplines (including human health, socio-economics, environment, hydrology, and energy) to provide investigators with a variety of baseline data to explore the multiple long-term effects of infrastructure development in the Brazilian Amazon.
Data for evaluation of fast kurtosis strategies, b-value optimization and exploration of diffusion MRI contrast
Authors: Hansen Brian , Jespersen Sune Nørhøj
Published: August 2016

Here we describe and provide diffusion magnetic resonance imaging (dMRI) data that was acquired in neural tissue and a physical phantom. Data acquired in biological tissue includes: fixed rat brain (acquired at 9.4 T) and spinal cord (acquired at 16.4 T) and in normal human brain (acquired at 3 T). This data was recently used for evaluation of diffusion kurtosis imaging (DKI) contrasts and for comparison to diffusion tensor imaging (DTI) parameter contrast. The data has also been used to optimize b-values for ex vivo and in vivo fast kurtosis imaging. The remaining data was obtained in a physical phantom with three orthogonal fiber orientations (fresh asparagus stems) for exploration of the kurtosis fractional anisotropy. However, the data may have broader interest and, collectively, may form the basis for image contrast exploration and simulations based on a wide range of dMRI analysis strategies.
Combinatory annotation of cell membrane receptors and signalling pathways of Bombyx mori prothoracic glands
Authors: Moulos Panagiotis , Samiotaki Martina , Panayotou George , Dedos Skarlatos G.
Published: August 2016

The cells of prothoracic glands (PG) are the main site of synthesis and secretion of ecdysteroids, the biochemical products of cholesterol conversion to steroids that shape the morphogenic development of insects. Despite the availability of genome sequences from several insect species and the extensive knowledge of certain signalling pathways that underpin ecdysteroidogenesis, the spectrum of signalling molecules and ecdysteroidogenic cascades is still not fully comprehensive. To fill this gap and obtain the complete list of cell membrane receptors expressed in PG cells, we used combinatory bioinformatic, proteomic and transcriptomic analysis and quantitative PCR to annotate and determine the expression profiles of genes identified as putative cell membrane receptors of the model insect species, Bombyx mori, and subsequently enrich the repertoire of signalling pathways that are present in its PG cells. The genome annotation dataset we report here highlights modules and pathways that may be directly involved in ecdysteroidogenesis and aims to disseminate data and assist other researchers in the discovery of the role of such receptors and their ligands.
Inhibition of nuclear factor kappaB proteins-platinated DNA interactions correlates with cytotoxic effectiveness of the platinum complexes
Authors: Brabec Viktor , Kasparkova Jana , Kostrhunova Hana , Farrell Nicholas P.
Published: August 2016

Nuclear DNA is the target responsible for anticancer activity of platinum anticancer drugs. Their activity is mediated by altered signals related to programmed cell death and the activation of various signaling pathways. An example is activation of nuclear factor kappaB (NF-κB). Binding of NF-κB proteins to their consensus sequences in DNA (κB sites) is the key biochemical activity responsible for the biological functions of NF-κB. Using gel-mobility-shift assays and surface plasmon resonance spectroscopy we examined the interactions of NF-κB proteins with oligodeoxyribonucleotide duplexes containing κB site damaged by DNA adducts of three platinum complexes. These complexes markedly differed in their toxic effects in tumor cells and comprised highly cytotoxic trinuclear platinum(II) complex BBR3464, less cytotoxic conventional cisplatin and ineffective transplatin. The results indicate that structurally different DNA adducts of these platinum complexes exhibit a different efficiency to affect the affinity of the platinated DNA (κB sites) to NF-κB proteins. Our results support the hypothesis that structural perturbations induced in DNA by platinum(II) complexes correlate with their higher efficiency to inhibit binding of NF-κB proteins to their κB sites and cytotoxicity as well. However, the full generalization of this hypothesis will require to evaluate a larger series of platinum(II) complexes.
Hyperuricemia in acute gastroenteritis is caused by decreased urate excretion via ABCG2
Authors: Matsuo Hirotaka , Tsunoda Tomoyuki , Ooyama Keiko , Sakiyama Masayuki , Sogo Tsuyoshi , Takada Tappei , Nakashima Akio , Nakayama Akiyoshi , Kawaguchi Makoto , Higashino Toshihide , Wakai Kenji , Ooyama Hiroshi , Hokari Ryota , Suzuki Hiroshi , Ichida Kimiyoshi , Inui Ayano , Fujimori Shin , Shinomiya Nariyoshi
Published: August 2016

To clarify the physiological and pathophysiological roles of intestinal urate excretion via ABCG2 in humans, we genotyped ABCG2 dysfunctional common variants, Q126X (rs72552713) and Q141K (rs2231142), in end-stage renal disease (hemodialysis) and acute gastroenteritis patients, respectively. ABCG2 dysfunction markedly increased serum uric acid (SUA) levels in 106 hemodialysis patients (P = 1.1 × 10−4), which demonstrated the physiological role of ABCG2 for intestinal urate excretion because their urate excretion almost depends on intestinal excretion via ABCG2. Also, ABCG2 dysfunction significantly elevated SUA in 67 acute gastroenteritis patients (P = 6.3 × 10−3) regardless of the degree of dehydration, which demonstrated the pathophysiological role of ABCG2 in acute gastroenteritis. These findings for the first time show ABCG2-mediated intestinal urate excretion in humans, and indicates the physiological and pathophysiological importance of intestinal epithelium as an excretion pathway besides an absorption pathway. Furthermore, increased SUA could be a useful marker not only for dehydration but also epithelial impairment of intestine.
Knowledge discovery from high-frequency stream nitrate concentrations: hydrology and biology contributions
Authors: Aubert Alice H. , Thrun Michael C. , Breuer Lutz , Ultsch Alfred
Published: August 2016

High-frequency, in-situ monitoring provides large environmental datasets. These datasets will likely bring new insights in landscape functioning and process scale understanding. However, tailoring data analysis methods is necessary. Here, we detach our analysis from the usual temporal analysis performed in hydrology to determine if it is possible to infer general rules regarding hydrochemistry from available large datasets. We combined a 2-year in-stream nitrate concentration time series (time resolution of 15 min) with concurrent hydrological, meteorological and soil moisture data. We removed the low-frequency variations through low-pass filtering, which suppressed seasonality. We then analyzed the high-frequency variability component using Pareto Density Estimation, which to our knowledge has not been applied to hydrology. The resulting distribution of nitrate concentrations revealed three normally distributed modes: low, medium and high. Studying the environmental conditions for each mode revealed the main control of nitrate concentration: the saturation state of the riparian zone. We found low nitrate concentrations under conditions of hydrological connectivity and dominant denitrifying biological processes, and we found high nitrate concentrations under hydrological recession conditions and dominant nitrifying biological processes. These results generalize our understanding of hydro-biogeochemical nitrate flux controls and bring useful information to the development of nitrogen process-based models at the landscape scale.
Emergence of metapopulations and echo chambers in mobile agents
Authors: Starnini Michele , Frasca Mattia , Baronchelli Andrea
Published: August 2016

Multi-agent models often describe populations segregated either in the physical space, i.e. subdivided in metapopulations, or in the ecology of opinions, i.e. partitioned in echo chambers. Here we show how both kinds of segregation can emerge from the interplay between homophily and social influence in a simple model of mobile agents endowed with a continuous opinion variable. In the model, physical proximity determines a progressive convergence of opinions but differing opinions result in agents moving away from each others. This feedback between mobility and social dynamics determines the onset of a stable dynamical metapopulation scenario where physically separated groups of like-minded individuals interact with each other through the exchange of agents. The further introduction of confirmation bias in social interactions, defined as the tendency of an individual to favor opinions that match his own, leads to the emergence of echo chambers where different opinions coexist also within the same group. We believe that the model may be of interest to researchers investigating the origin of segregation in the offline and online world.
Identification of lead chemotherapeutic agents from medicinal plants against blood flukes and whipworms
Authors: Wangchuk Phurpa , Giacomin Paul R. , Pearson Mark S. , Smout Michael J. , Loukas Alex
Published: August 2016

Schistosomiasis and trichuriasis are two of the most common neglected tropical diseases (NTD) that affect almost a billion people worldwide. There is only a limited number of effective drugs to combat these NTD. Medicinal plants are a viable source of parasiticides. In this study, we have investigated six of the 19 phytochemicals isolated from two Bhutanese medicinal plants, Corydalis crispa and Pleurospermum amabile, for their anthelmintic properties. We used the xWORM technique and Scanning Electron Microscope-based imaging to determine the activity of the compounds. Of the six compounds tested, isomyristicin and bergapten showed significant anthelmintic activity against Schistosoma mansoni and Trichuris muris with bergapten being the most efficacious compound one against both parasites (S. mansoni IC50 = 8.6 μg/mL and T. muris IC50 = 10.6 μg/mL) and also against the schistosomulum stage of S. mansoni. These two compounds induced tegumental damage to S. mansoni and affected the cuticle, bacillary bands and bacillary glands of T. muris. The efficacy against multiple phylogenetically distinct parasites and different life stages, especially the schistosomulum where praziquantel is ineffective, makes isomyristicin and bergapten novel scaffolds for broad-spectrum anthelmintic drug development that could be used for the control of helminths infecting humans and animals.
A novel inhibitory effect of oxazol-5-one compounds on ROCKII signaling in human coronary artery vascular smooth muscle cells
Authors: Al-Ghabkari Abdulhameed , Deng Jing-Ti , McDonald Paul C. , Dedhar Shoukat , Alshehri Mana , Walsh Michael P. , MacDonald Justin A.
Published: August 2016

The selectivity of (4Z)-2-(4-chloro-3-nitrophenyl)-4-(pyridin-3-ylmethylidene)-1,3-oxazol-5-one (DI) for zipper-interacting protein kinase (ZIPK) was previously described by in silico computational modeling, screening a large panel of kinases and determining the inhibition efficacy. Our assessment of DI revealed another target, the Rho-associated coiled-coil-containing protein kinase 2 (ROCKII). In vitro studies showed DI to be a competitive inhibitor of ROCKII (Ki, 132 nM with respect to ATP). This finding was supported by in silico molecular surface docking of DI with the ROCKII ATP-binding pocket. Time course analysis of myosin regulatory light chain (LC20) phosphorylation catalyzed by ROCKII in vitro revealed a significant decrease upon treatment with DI. ROCKII signaling was investigated in situ in human coronary artery vascular smooth muscle cells (CASMCs). ROCKII down-regulation using siRNA revealed several potential substrates involved in smooth muscle contraction (e.g., LC20, Par-4, MYPT1) and actin cytoskeletal dynamics (cofilin). The application of DI to CASMCs attenuated LC20, Par-4, LIMK and cofilin phosphorylations. Notably, cofilin phosphorylation was not significantly decreased with a novel ZIPK selective inhibitor (HS-38). In addition, CASMCs treated with DI underwent cytoskeletal changes that were associated with diminution of cofilin phosphorylation. We conclude that DI is not selective for ZIPK and is a potent inhibitor of ROCKII.
Theoretical Study of the ESIPT Process for a New Natural Product Quercetin
Authors: Yang Yunfan , Zhao Jinfeng , Li Yongqing
Published: August 2016

The investigation of excited-state intramolecular proton transfer (ESIPT) has been carried out via the density functional theory (DFT) and the time-dependent density functional theory (TDDFT) method for natural product quercetin in dichloromethane (DCM) solvent. For distinguishing different types of intramolecular interaction, the reduced density gradient (RDG) function also has been used. In this study, we have clearly clarified the viewpoint that two kinds of tautomeric forms (K1, K2)originated from ESIPT processconsist inthe first electronic excited state (S1). The phenomenon of hydrogen bonding interaction strengtheninghas been proved by comparing the changes of infrared (IR) vibrational spectra and bond parameters of the hydrogen bonding groups in the ground state with that in the first excited state. The frontier molecular orbitals (MOs)provided visual electron density redistribution have further verified the hydrogen bond strengthening mechanism. It should be noted that the ESIPT process of the K2form is easier to occur than that of the K1form via observing the potential energy profiles. Furthermore, the RDG isosurfaces has indicated that hydrogen bonding interaction of the K2form is stronger than that of the K1formin the S1state, which is also the reason why the ESIPT process of the K2form is easier to occur.
MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds
Authors: Li Xiang , Xia Kuaifei , Liang Zhen , Chen Kunling , Gao Caixia , Zhang Mingyong
Published: August 2016

Rice tillering has an important influence on grain yield, and is promoted by nitrogen (N) fertilizer. Several genes controlling rice tillering, which are regulated by poor N supply, have been identified. However, the molecular mechanism associated with the regulation of tillering based on N supply is poorly understood. Here, we report that rice microRNA393 (OsmiR393) is involved in N-mediated tillering by decreasing auxin signal sensitivity in axillary buds. Expression analysis showed that N fertilizer causes up-regulation of OsmiR393, but down-regulation of two target genes (OsAFB2 and OsTB1). In situ expression analysis showed that OsmiR393 is highly expressed in the lateral axillary meristem. OsmiR393 overexpression mimicked N-mediated tillering in wild type Zhonghua 11 (ZH11). Mutation of OsMIR393 in ZH11 repressed N-promoted tillering, which simulated the effects of limited N, and this could not be restored by supplying N fertilizer. Western blot analysis showed that OsIAA6 was accumulated in both OsmiR393-overexpressing lines and N-treated wild type rice, but was reduced in the OsMIR393 mutant. Therefore, we deduced that N-induced OsmiR393 accumulation reduces the expression of OsTIR1 and OsAFB2, which alleviates sensitivity to auxin in the axillary buds and stabilizes OsIAA6, thereby promoting rice tillering.
High Mobility Group Box Protein 1 Boosts Endothelial Albumin Transcytosis through the RAGE/Src/Caveolin-1 Pathway
Authors: Shang Dan , Peng Tao , Gou Shanmiao , Li Yiqing , Wu Heshui , Wang Chunyou , Yang Zhiyong
Published: August 2016

High-mobility group box protein 1 (HMGB1), an inflammatory mediator, has been reported to destroy cell-cell junctions, resulting in vascular endothelial hyperpermeability. Here, we report that HMGB1 increases the endothelial transcytosis of albumin. In mouse lung vascular endothelial cells (MLVECs), HMGB1 at a concentration of 500 ng/ml or less did not harm cell-cell junctions but rapidly induced endothelial hyperpermeability to 125I-albumin. HMGB1 induced an increase in 125I-albumin and AlexaFluor 488-labeled albumin internalization in endocytosis assays. Depletion of receptor for advanced glycation end products (RAGE), but not TLR2 or TLR4, suppressed HMGB1-induced albumin transcytosis and endocytosis. Genetic and pharmacological destruction of lipid rafts significantly inhibited HMGB1-induced albumin endocytosis and transcytosis. HMGB1 induced the rapid phosphorylation of caveolin (Cav)-1 and Src. Either RAGE gene silencing or soluble RAGE suppressed Cav-1 Tyr14 phosphorylation and Src Tyr418 phosphorylation. The Src inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo[3,4-d] pyrimidine (PP2) blocked HMGB1-induced Cav-1 Tyr14 phosphorylation. PP2 and overexpression of Cav-1 with a T14F mutation significantly inhibited HMGB1-induced transcytosis and albumin endocytosis. Our findings suggest that HMGB1 induces the transcytosis of albumin via RAGE-dependent Src phosphorylation and Cav-1 phosphorylation. These studies revealed a new mechanism of HMGB1-induced endothelial hyperpermeability.
Mammalian non-CG methylations are conserved and cell-type specific and may have been involved in the evolution of transposon elements
Authors: Guo Weilong , Zhang Michael Q. , Wu Hong
Published: August 2016

Although non-CG methylations are abundant in several mammalian cell types, their biological significance is sparsely characterized. We gathered 51 human and mouse DNA methylomes from brain neurons, embryonic stem cells and induced pluripotent stem cells, primordial germ cells and oocytes. We utilized an unbiased sub-motif prediction method and reported CW as the representative non-CG methylation context, which is distinct from CC methylation in terms of sequence context and genomic distribution. A two-dimensional comparison of non-CG methylations across cell types and species was performed. Unambiguous studies of sequence preferences and genomic region enrichment showed that CW methylation is cell-type specific and is also conserved between humans and mice. In brain neurons, it was found that active long interspersed nuclear element-1 (LINE-1) lacked CW methylations but not CG methylations. Coincidentally, both human Alu and mouse B1 elements preferred high CW methylations at specific loci during their respective evolutionary development. Last, the strand-specific distributions of CW methylations in introns and long interspersed nuclear elements are also cell-type specific and conserved. In summary, our results illustrate that CW methylations are highly conserved among species, are dynamically regulated in each cell type, and are potentially involved in the evolution of transposon elements.
Spatial limitations in averaging social cues
Authors: Florey Joseph , Clifford Colin W. G. , Dakin Steven , Mareschal Isabelle
Published: August 2016

The direction of social attention from groups provides stronger cueing than from an individual. It has previously been shown that both basic visual features such as size or orientation and more complex features such as face emotion and identity can be averaged across multiple elements. Here we used an equivalent noise procedure to compare observers’ ability to average social cues with their averaging of a non-social cue. Estimates of observers’ internal noise (uncertainty associated with processing any individual) and sample-size (the effective number of gaze-directions pooled) were derived by fitting equivalent noise functions to discrimination thresholds. We also used reverse correlation analysis to estimate the spatial distribution of samples used by participants. Averaging of head-rotation and cone-rotation was less noisy and more efficient than averaging of gaze direction, though presenting only the eye region of faces at a larger size improved gaze averaging performance. The reverse correlation analysis revealed greater sampling areas for head rotation compared to gaze. We attribute these differences in averaging between gaze and head cues to poorer visual processing of faces in the periphery. The similarity between head and cone averaging are examined within the framework of a general mechanism for averaging of object rotation.
Novel facile method for obtaining CdSe/polyaniline/C60 composite materials
Authors: Rusen Edina , Diacon Aurel , Mocanu Alexandra , Nistor Leona Cristina
Published: August 2016

This study presents a novel method for the oxidative polymerization of aniline (ANI) by employing fullerene C60/cadmium selenide (CdSe) quantum dots, as promoting agent of the polymerization system. The polymerization initiation mechanism is based on the difference between the HOMO-LUMO energy levels of the components which permits the formation of a continuous donor-acceptor exchange. Both the polymerization reaction evolution and the molecular weights of the obtained polymers have been characterized. The novelty of the paper consists in the synthesis of a novel nano-composite material through a novel polymerization technique. The resulting material containing PANI, CdSe quantum dots and C60 has been characterized by UV-Vis, NIR, fluorescence, TEM and GPC analyses.