bluuebunny/crossref_metadata_2025 huggingface dataset:
Rethinking the Fertility Transition in Rural Aragón (Spain) Using Height Data
Authors: Marco-Gracia Francisco J. , López-Antón Margarita
Published: August 2021

Based on an analysis of the life trajectories of 2510 conscripts and their families from a Spanish rural area in the period 1835–1977, this paper studies the development of the fertility transition in relation to height using bivariate analyses. The use of heights is an innovative perspective of delving into the fertility transition and social transformation entailed. The results confirm that the men with a low level of biological well-being (related to low socio-economic groups) were those who started to control their fertility, perhaps due to the effect that increased average family size had on their budget. The children of individuals who controlled their fertility were taller than the children of other families. Therefore, the children of parents who controlled their fertility experienced the largest intergenerational increase in height (approximately 50% higher). This increase could be due to the consequence of a greater investment in children (Becker’s hypothesis) or a greater availability of resources for the whole family (resource dilution hypothesis).
Analysing the Impact of Large Data Imports in OpenStreetMap
Authors: Witt Raphael , Loos Lukas , Zipf Alexander
Published: August 2021

OpenStreetMap (OSM) is a global mapping project which generates free geographical information through a community of volunteers. OSM is used in a variety of applications and for research purposes. However, it is also possible to import external data sets to OpenStreetMap. The opinions about these data imports are divergent among researchers and contributors, and the subject is constantly discussed. The question of whether importing data, especially large quantities, is adding value to OSM or compromising the progress of the project needs to be investigated more deeply. For this study, OSM’s historical data were used to compute metrics about the developments of the contributors and OSM data during large data imports which were for the Netherlands and India. Additionally, one time period per study area during which there was no large data import was investigated to compare results. For making statements about the impacts of large data imports in OSM, the metrics were analysed using different techniques (cross-correlation and changepoint detection). It was found that the contributor activity increased during large data imports. Additionally, contributors who were already active before a large import were more likely to contribute to OSM after said import than contributors who made their first contributions during the large data import. The results show the difficulty of interpreting a heterogeneous data source, such as OSM, and the complexity of the project. Limitations and challenges which were encountered are explained, and future directions for continuing in this field of research are given.
Functional Characterization of Multiple Ehrlichia chaffeensis Sodium (Cation)/Proton Antiporter Genes Involved in the Bacterial pH Homeostasis
Authors: Wei Lanjing , Liu Huitao , Alizadeh Kimia , Juarez-Rodriguez Maria D. , Ganta Roman R.
Published: August 2021

Ehrlichia chaffeensis causes human monocytic ehrlichiosis. Little is known about how this and other related tick-borne rickettsia pathogens maintain pH homeostasis in acidified phagosomes and the extracellular milieu. The membrane-bound sodium (cation)/proton antiporters are found in a wide range of organisms aiding pH homeostasis. We recently reported a mutation in an antiporter gene of E. chaffeensis (ECH_0379) which causes bacterial in vivo attenuation. The E. chaffeensis genome contains 10 protein coding sequences encoding for predicted antiporters. We report here that nine of these genes are transcribed during the bacterial growth in macrophages and tick cells. All E. chaffeensis antiporter genes functionally complemented antiporter deficient Escherichia coli. Antiporter activity for all predicted E. chaffeensis genes was observed at pH 5.5, while gene products of ECH_0179 and ECH_0379 were also active at pH 8.0, and ECH_0179 protein was complemented at pH 7.0. The antiporter activity was independently verified for the ECH_0379 protein by proteoliposome diffusion analysis. This is the first description of antiporters in E. chaffeensis and demonstrates that the pathogen contains multiple antiporters with varying biological functions, which are likely important for the pH homeostasis of the pathogen’s replicating and infectious forms.
Ethanol Induces Extracellular Vesicle Secretion by Altering Lipid Metabolism through the Mitochondria-Associated ER Membranes and Sphingomyelinases
Authors: Ibáñez Francesc , Montesinos Jorge , Area-Gomez Estela , Guerri Consuelo , Pascual María
Published: August 2021

Recent evidence pinpoints extracellular vesicles (EVs) as key players in intercellular communication. Given the importance of cholesterol and sphingomyelin in EV biology, and the relevance of mitochondria-associated endoplasmic reticulum membranes (MAMs) in cholesterol/sphingomyelin homeostasis, we evaluated if MAMs and sphingomyelinases (SMases) could participate in ethanol-induced EV release. EVs were isolated from the extracellular medium of BV2 microglia treated or not with ethanol (50 and 100 mM). Radioactive metabolic tracers combined with thin layer chromatography were used as quantitative methods to assay phospholipid transfer, SMase activity and cholesterol uptake/esterification. Inhibitors of SMase (desipramine and GW4869) and MAM (cyclosporin A) activities were also utilized. Our data show that ethanol increases the secretion and inflammatory molecule concentration of EVs. Ethanol also upregulates MAM activity and alters lipid metabolism by increasing cholesterol uptake, cholesterol esterification and SMase activity in microglia. Notably, the inhibition of either SMase or MAM activity prevented the ethanol-induced increase in EV secretion. Collectively, these results strongly support a lipid-driven mechanism, specifically via SMases and MAM, to explain the effect of ethanol on EV secretion in glial cells.
Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis from Dermatophagoides farinae
Authors: Kim Geunyeong , Hong Minhwa , Kashif Ayesha , Hong Yujin , Park Beom-Seok , Mun Ji-Young , Choi Hyosun , Lee Ji-Sook , Yang Eun-Ju , Woo Ran-Sook , Lee Soo-Jin , Yang Minseo , Kim In-Sik
Published: August 2021

It is difficult to treat allergic diseases including asthma completely because its pathogenesis remains unclear. House dust mite (HDM) is a critical allergen and Toll-like receptor (TLR) 4 is a member of the toll-like receptor family, which plays an important role in allergic diseases. The purpose of this study was to characterize a novel allergen, Der f 38 binding to TLR4, and unveil its role as an inducer of allergy. Der f 38 expression was detected in the body and feces of Dermatophagoides farinae (DF). Electron microscopy revealed that it was located in the granule layer, the epithelium layer, and microvilli of the posterior midgut. The skin prick test showed that 60% of allergic subjects were Der f 38-positive. Der f 38 enhanced surface 203c expression in basophils of Der f 38-positive allergic subjects. By analysis of the model structure of Der p 38, the expected epitope sites are exposed on the exterior side. In animal experiments, Der f 38 triggered an infiltration of inflammatory cells. Intranasal (IN) administration of Der f 38 increased neutrophils in the lung. Intraperitoneal (IP) and IN injections of Der f 38 induced both eosinophils and neutrophils. Increased total IgE level and histopathological features were found in BALB/c mice treated with Der f 38 by IP and IN injections. TLR4 knockout (KO) BALB/c mice exhibited less inflammation and IgE level in the sera compared to wild type (WT) mice. Der f 38 directly binds to TLR4 using biolayer interferometry. Der f 38 suppressed the apoptosis of neutrophils and eosinophils by downregulating proteins in the proapoptotic pathway including caspase 9, caspase 3, and BAX and upregulating proteins in the anti-apoptotic pathway including BCL-2 and MCL-1. These findings might shed light on the pathogenic mechanisms of allergy to HDM.
Regulatory Mechanisms of Anthocyanin Biosynthesis in Apple and Pear
Authors: Liu Huimin , Liu Zijin , Wu Yu , Zheng Lamei , Zhang Genfa
Published: August 2021

Anthocyanins contribute to the quality and flavour of fruits. They are produced through the phenylpropanoid pathway, which is regulated by specific key genes that have been identified in many species. The dominant anthocyanin forms are reversibly transformed at different pH states, thus forming different colours in aqueous solutions. In plants, anthocyanins are controlled by specific factors of the biosynthetic pathway: light, temperature, phytohormones and transcription factors. Although great progress in research on anthocyanin structures and the regulation of anthocyanin biosynthesis has been made, the molecular regulatory mechanisms of anthocyanin biosynthesis in different plants remain less clear. In addition, the co-regulation of anthocyanin biosynthesis is poorly understood. In this review, we summarise previous findings on anthocyanin biosynthesis, including the biochemical and biological features of anthocyanins; differences in anthocyanin biosynthesis among fruit species, i.e., apple, red pear, and the model plant Arabidopsis thaliana; and the developmental and environmental regulation of anthocyanin accumulation. This review reveals the molecular mechanisms underlying anthocyanin biosynthesis in different plant species and provides valuable information for the development of anthocyanin-rich red-skinned and red-fleshed apple and pear varieties.
Nasal Rifampicin Improves Cognition in a Mouse Model of Dementia with Lewy Bodies by Reducing α-Synuclein Oligomers
Authors: Umeda Tomohiro , Hatanaka Yukari , Sakai Ayumi , Tomiyama Takami
Published: August 2021

α-Synuclein oligomers are thought to play an important role in the pathogenesis of dementia with Lewy bodies (DLB). There is no effective cure for DLB at present. Previously, we demonstrated that in APP- and tau-transgenic mice, oral or intranasal rifampicin reduced brain Aβ and tau oligomers and improved mouse cognition. In the present study, we expanded our research to DLB. Rifampicin was intranasally administered to 6-month-old A53T-mutant α-synuclein-transgenic mice at 0.1 mg/day for 1 month. The mice displayed memory impairment but no motor deficit at this age, indicating a suitable model of DLB. α-Synuclein pathologies were examined by the immunohistochemical/biochemical analyses of brain tissues. Cognitive function was evaluated by the Morris water maze test. Intranasal rifampicin significantly reduced the levels of [pSer129] α-synuclein in the hippocampus and α-synuclein oligomers in the visual cortex and hippocampus. The level of the presynaptic marker synaptophysin in the hippocampus was recovered to the level in non-transgenic littermates. In the Morris water maze, a significant improvement in spatial reference memory was observed in rifampicin-treated mice. Taken together with our previous findings, these results suggest that intranasal rifampicin is a promising remedy for the prevention of neurodegenerative dementia, including Alzheimer’s disease, frontotemporal dementia, and DLB.
Non-Rodent Genetic Animal Models for Studying Tauopathy: Review of Drosophila, Zebrafish, and C. elegans Models
Authors: Giong Hoi-Khoanh , Subramanian Manivannan , Yu Kweon , Lee Jeong-Soo
Published: August 2021

Tauopathy refers to a group of progressive neurodegenerative diseases, including frontotemporal lobar degeneration and Alzheimer’s disease, which correlate with the malfunction of microtubule-associated protein Tau (MAPT) due to abnormal hyperphosphorylation, leading to the formation of intracellular aggregates in the brain. Despite extensive efforts to understand tauopathy and develop an efficient therapy, our knowledge is still far from complete. To find a solution for this group of devastating diseases, several animal models that mimic diverse disease phenotypes of tauopathy have been developed. Rodents are the dominating tauopathy models because of their similarity to humans and established disease lines, as well as experimental approaches. However, powerful genetic animal models using Drosophila, zebrafish, and C. elegans have also been developed for modeling tauopathy and have contributed to understanding the pathophysiology of tauopathy. The success of these models stems from the short lifespans, versatile genetic tools, real-time in-vivo imaging, low maintenance costs, and the capability for high-throughput screening. In this review, we summarize the main findings on mechanisms of tauopathy and discuss the current tauopathy models of these non-rodent genetic animals, highlighting their key advantages and limitations in tauopathy research.
Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice
Authors: Lewandowska Hanna , Eljaszewicz Andrzej , Poplawska Izabela , Tynecka Marlena , Walewska Alicja , Grubczak Kamil , Holl Jordan , Razak Hady Hady , Czaban Slawomir Lech , Reszec Joanna , Przybytniak Grażyna , Głuszewski Wojciech , Sadło Jarosław , Dąbrowska-Gralak Małgorzata , Kowalewski Cezary , Fiedor Piotr , Oldak Tomasz , Kaminski Artur , Zimek Zbigniew , Moniuszko Marcin
Published: August 2021

Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM; however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential.
Triggerfish Recording of IOP Patterns in Combined HFDS Minimally Invasive Glaucoma and Cataract Surgery: A Prospective Study
Authors: Pajic Bojan , Resan Mirko , Pajic-Eggspuehler Brigitte , Massa Horace , Cvejic Zeljka
Published: August 2021

Background: The aim of the study is to investigate whether the circadian IOP rhythm can be influenced by combined cataract surgery with high frequency deep sclerotomy (HFDS) and whether intraocular pressure (IOP) can be significantly reduced by HFDS. Methods: In our study 10 patients were included, in whom 24 h IOP monitoring was installed before and after HFDS/cataract surgery using a Triggerfish. HFDS is a minimally invasive glaucoma surgery (MIGS). Results: After performed HFDS combined with cataract surgery, the IOP was reduced from 27.7 ± 2.11 mmHg to 14.4 ± 2.59 mmHg, which is highly significant (p < 0.001). The contact lens sensor (CLS) cosinor analysis pre- and postoperatively showed that the circadian rhythm is not influenced by the surgery, i.e., the circadian IOP rhythm did not show significant differences before and after surgery. Conclusions: HFDS combined with cataract surgery is a potent surgical method that can significantly reduce the IOP. However, the circadian rhythm cannot be changed by the surgery. The acrophase remained during the night in all patients.
Etiological Work-Up for Adults with Bronchiectasis: A Predictive Diagnostic Score for Primary Ciliary Dyskinesia and Cystic Fibrosis
Authors: Schlemmer Frederic , Hamzaoui Agnes , Zebachi Sonia , Le Thuaut Aurelie , Mangiapan Gilles , Monnet Isabelle , Boudjema Amel , Jabot Laurence , Housset Bruno , Bastuji-Garin Sylvie , Bassinet Laurence , Maitre Bernard
Published: August 2021

Background: etiological investigations are not done for all adult patients with bronchiectasis because of the availability and interpretation of tests. The aim of the study was to elaborate a score to identify patients at high risk of having cystic fibrosis or primary ciliary dyskinesia (CF/PCD), which require appropriate management. Methods: diagnostic work-ups were carried out on a French monocenter cohort, and results were subjected to logistic-regression analyses to identify the independent factors associated with CF/PCD diagnosis and, thereby, elaborate a score to validate in a second cohort. Results: among 188 patients, 158 had no obvious diagnosis and were enrolled in the algorithm-construction group. In multivariate analyses, age at symptom onset (8.69 (2.10–35.99); p = 0.003), chronic ENT symptoms or diagnosed sinusitis (10.53 (1.26–87.57); p = 0.03), digestive symptoms or situs inversus (5.10 (1.23–21.14); p = 0.025), and Pseudomonas. aeruginosa and/or Staphylococcus aureus isolated from sputum (11.13 (1.34–92.21); p = 0.02) are associated with CF or PCD. Receiver operating characteristics curve analysis, using a validation group of 167 patients with bronchiectasis, confirmed the score’s performance with AUC 0.92 (95% CI: 0.84–0.98). Conclusions: a clinical score may help identify adult patients with bronchiectasis at higher risk of having CF or PCD.
Current Status of Next-Generation Sequencing-Based Cancer Genome Profiling Tests in Japan and Prospects for Liquid Biopsy
Authors: Yoshii Yumi , Okazaki Shunsuke , Takeda Masayuki
Published: August 2021

Next-generation sequencing-based comprehensive genome profiling (CGP) testing, OncoGuide NCC Oncopanel System, and FoundationOne CDx Cancer Genomic Profile have been covered by the Japanese national health insurance system since June 2019. Because CGP was initially developed to enroll patients into an early-phase clinical trial for solid tumors, its approved indications have been limited to patients who have completed the standard chemotherapy treatment. Approximately 14,000 cases have been registered with the Center for Cancer Genomics and Advanced Therapeutics as of March 2021. Measuring the drug access rate is not enough due to patients’ deteriorating condition during CGP analysis and due to the limited number of ongoing clinical trials available, although tumor-agnostic therapies, such as the use of pembrolizumab in high microsatellite-instable solid tumors and in conditions with a high tumor mutational burden (≥10 mut/Mb) as well as the use of entrectinib and larotrectinib in NTRK fusion-positive tumors have been approved in Japan. Moreover, since this analysis is performed using DNA derived from tumor tissue, it is difficult to perform CGP in cases in which an insufficient amount of tissue exists. Thus, noninvasive blood-based assays have been developed, and CGP panels using circulating tumor DNA from blood were approved in March 2021. However, cost, timing, and the number of tests allowed by the health system have not yet been determined. Therefore, in this review, we outline the current status and issues of CGP testing using tumor tissues as well as the expectations and limitations of liquid biopsy for use in Japanese clinical practice.
Multi-Perspective Analysis of Building Orientation Effects on Microstructure, Mechanical and Surface Properties of SLM Ti6Al4V with Specific Geometry
Authors: Cai Wentong , Song Qinghua , Ji Hansong , Gupta Munish Kumar
Published: August 2021

Building orientation is important in selective laser melting (SLM) processes. Current studies only focus on the horizontal and vertical building orientations without considering different modes of horizontal orientations. In fact, for horizontal orientation, different surfaces of the sample that contact the substrate will affect the heat transfer mode and efficiency, and in turn affect the microstructure and material properties. In this paper, the effect of two modes of horizontal building orientations on microstructure, mechanical and surface properties of SLM Ti6Al4V was studied. Current research about building orientation is deficient because the geometry of samples or test surfaces are not strictly defined, which seriously influences the results due to their different heat transfer efficiency and mode. Therefore, the geometry of the samples and test surfaces were clearly defined, and its necessity was proved in this study. To achieve the research goal, three test samples were prepared: sample SLM-PB-S with the building orientation parallel to the substrate and the shorter side L1 contacts it, sample SLM-PB-L with the building orientation parallel to the substrate and the longer side L2 contacts it and sample SLM-VB with the building orientation vertical to the substrate. Subsequently, the microstructure, grain information, densification, residual stress, micro-hardness, tensile properties and surface topography of different samples were analyzed and compared. In the results, SLM-PB-S exhibited denser microstructure and better mechanical properties than SLM-PB-L, including smaller grain size, stronger texture, higher density, micro-hardness, tensile strength, plasticity and better surface quality. It originates from a higher cooling rate and shorter scanning time between layers during SLM-PB-S fabrication, leading to finer grains, lower porosity and better interlayer metallurgical bonding, thus resulting in better material properties. This study can provide a reference to select the proper building orientation in SLM.
X-ray Dose Rate and Spectral Measurements during Ultrafast Laser Machining Using a Calibrated (High-Sensitivity) Novel X-ray Detector
Authors: Mosel Philip , Sankar Pranitha , Düsing Jan Friedrich , Dittmar Günter , Püster Thomas , Jäschke Peter , Vahlbruch Jan-Willem , Morgner Uwe , Kovacev Milutin
Published: August 2021

Ultrashort pulse laser machining is subject to increase the processing speeds by scaling average power and pulse repetition rate, accompanied with higher dose rates of X-ray emission generated during laser–matter interaction. In particular, the X-ray energy range below 10 keV is rarely studied in a quantitative approach. We present measurements with a novel calibrated X-ray detector in the detection range of 2–20 keV and show the dependence of X-ray radiation dose rates and the spectral emissions for different laser parameters from frequently used metals, alloys, and ceramics for ultrafast laser machining. Our investigations include the dose rate dependence on various laser parameters available in ultrafast laser laboratories as well as on industrial laser systems. The measured X-ray dose rates for high repetition rate lasers with different materials definitely exceed the legal limitations in the absence of radiation shielding.
Empowering Materials Processing and Performance from Data and AI
Authors: Chinesta Francisco , Cueto Elias , Klusemann Benjamin
Published: August 2021

Third millennium engineering is addressing new challenges in materials sciences and engineering [...]
Compressive Properties of Functionally Graded Bionic Bamboo Lattice Structures Fabricated by FDM
Authors: Wen Zhou , Li Ming
Published: August 2021

Bionic design is considered a promising approach to improve the performance of lattice structures. In this work, bamboo-inspired cubic and honeycomb lattice structures with graded strut diameters were designed and manufactured by 3D printing. Uniform lattice structures were also designed and fabricated for comparison. Quasi-static compression tests were conducted on lattice structures, and the effects of the unit cell and structure on the mechanical properties, energy absorption and deformation mode were investigated. Results indicated that the new bionic bamboo structure showed similar mechanical properties and energy absorption capacity to the honeycomb structure but performed better than the cubic structure. Compared with the uniform lattice structures, the functionally graded lattice structures showed better performance in terms of initial peak strength, compressive modulus and energy absorption.
The Relationship between Tribo-Magnetization and Wear State during Reciprocating Sliding
Authors: Zhao Kunpeng , Fan Jianchun , Zhang Laibin
Published: August 2021

In order to investigate the relationship between tribo-magnetization and wear phenomena, a series of sliding-friction tests were carried out using a pin-on-block reciprocator type device, which was specially designed to generate sliding friction and to detect magnetic fields. The tribological behavior was observed by a charge-coupled device (CCD) camera in real time, and the magnetic signal was detected by using a two-axis magnetic sensor. It was found that as the friction continues, both the tangential and the normal components of magnetic field on the material surface increase gradually, and that both the average value of the tangential magnetic field ΔH(x)mean and the peak-to-peak value of the normal magnetic field Vpp at the wear scar clearly reflect changes of the wear state. Furthermore, in order to better understand the tribo-magnetization phenomenon, the changes of surface morphology and wear debris in different wear stages were analyzed. Moreover, an improved magnetic dipole model that considered the distribution of magnetic charges on the worn surface was established. This model can successfully describe the tribo-magnetization phenomenon during the stable wear stage, and should be useful for characterizing frictional wear phenomena in the future.
A Soft Pressure Sensor Array Based on a Conducting Nanomembrane
Authors: Jung Daekwang , Kang Kyumin , Jung Hyunjin , Seong Duhwan , An Soojung , Yoon Jiyong , Kim Wooseok , Shin Mikyung , Baac Hyoung , Won Sangmin , Shin Changhwan , Son Donghee
Published: August 2021

Although skin-like pressure sensors exhibit high sensitivity with a high performance over a wide area, they have limitations owing to the critical issue of being linear only in a narrow strain range. Various strategies have been proposed to improve the performance of soft pressure sensors, but such a nonlinearity issue still exists and the sensors are only effective within a very narrow strain range. Herein, we fabricated a highly sensitive multi-channel pressure sensor array by using a simple thermal evaporation process of conducting nanomembranes onto a stretchable substrate. A rigid-island structure capable of dissipating accumulated strain energy induced by external mechanical stimuli was adopted for the sensor. The performance of the sensor was precisely controlled by optimizing the thickness of the stretchable substrate and the number of serpentines of an Au membrane. The fabricated sensor exhibited a sensitivity of 0.675 kPa−1 in the broad pressure range of 2.3–50 kPa with linearity (~0.990), and good stability (>300 Cycles). Finally, we successfully demonstrated a mapping of pressure distribution.
Effects of Seed-Layer N2O Plasma Treatment on ZnO Nanorod Based Ultraviolet Photodetectors: Experimental Investigation with Two Different Device Structures
Authors: Lee Seungmin , Nam Kiyun , Kim Jae Hyun , Hong Gi Young , Kim Sam-Dong
Published: August 2021

The crystalline quality of ZnO NR (nanorod) as a sensing material for visible blind ultraviolet PDs (photodetectors) critically depends on the SL (seed layer) material of properties, which is a key to high-quality nanocrystallite growth, more so than the synthesis method. In this study, we fabricated two different device structures of a gateless AlGaN/GaN HEMT (high electron mobility transistor) and a photoconductive PD structure with an IDE (interdigitated electrode) pattern implemented on a PET (polyethylene terephthalate) flexible substrate, and investigated the impact on device performance through the SL N2O plasma treatment. In case of HEMT-based PD, the highest current on-off ratio (~7) and spectral responsivity R (~1.5 × 105 A/W) were obtained from the treatment for 6 min, whereas the IDE pattern-based PD showed the best performance (on-off ratio = ~44, R = ~69 A/W) from the treatment for 3 min and above, during which a significant etch damage on PET substrates was produced. This improvement in device performance was due to the enhancement in NR crystalline quality as revealed by our X-ray diffraction, photoluminescence, and microanalysis.
The Effects of a Nutrition Education Intervention on Sports Nutrition Knowledge during a Competitive Season in Highly Trained Adolescent Swimmers
Authors: Foo Wee Lun , Faghy Mark A. , Sparks Andy , Newbury Josh W. , Gough Lewis A.
Published: August 2021

The aim of this study was to evaluate the effects of a seven-week nutrition education intervention on the sports nutrition knowledge (SNK) of highly trained UK adolescent swimmers. Fifteen national and international adolescent swimmers (males = 5; females = 10, 15.5 ± 1.1 years, 170.2 ± 7.5 cm, 60.3 ± 5.7 kg) participated in the study during seven consecutive weeks of the competitive swimming season. The participants received 30 min of nutrition education once per week in a classroom-based setting after they had completed their regular swim training. An undergraduate sports nutrition student delivered all nutrition education sessions and SNK questionnaires were administered to the participants pre- and post-intervention. The mean total SNK score improved by 8.3% (SD = 8.4%, 95% CI = 4.1–12.6; p = 0.006; ES = 1.0) following the nutrition education sessions. On an individual basis, ten swimmers significantly improved their total SNK score, whereas four swimmers did not improve, and one swimmer performed significantly worse after the intervention. Moreover, the swimmers’ knowledge of hydration improved by 22.2% (SD = 20.6%, 95% CI = 11.8–32.6, p = 0.004, ES = 1.1) over the seven-week timeframe, which was the only nutrition topic to have a significantly increased knowledge score. The current study therefore suggests that a nutrition education intervention can positively influence the SNK of highly trained adolescent swimmers.
Temperatures Influence Susceptibility to Insecticides in Aedes aegypti and Aedes albopictus (Diptera: Culicidae) Mosquitoes
Authors: Salinas Wendy S. , Feria-Arroyo Teresa P. , Vitek Christopher J.
Published: August 2021

Aedes aegypti and Aedes albopictus (Diptera: Culicidae) are vectors for several arboviruses, including dengue, Zika virus and chikungunya virus. The primary method of controlling these diseases is controlling the vector population, often with insecticides. Insecticide resistance may impact the success of these efforts. We tested the effect of variable temperature exposures on susceptibility to insecticides by exposing adult A.aegypti and A. albopictus to different temperatures and tested their susceptibility to insecticides. We hypothesized that adults maintained at high temperatures would show increased susceptibility to insecticides relative to lower temperatures. Colony mosquitoes were hatched, reared to adulthood and then maintained in three temperature regimes that reflect average seasonal temperatures in the Rio Grande Valley, TX. Susceptibility to permethrin and deltamethrin was assessed using the CDC bottle bioassay method. Overall Aedes albopictus had higher susceptibility to all insecticides than Aedes aegypti. Mosquitoes kept at different temperatures exhibited differential susceptibility to insecticides. Low temperature exposed mosquitoes had decreased susceptibility while high temperature conditions resulted in increased mortality. Our results suggest public health officials must consider temperature effects when controlling mosquitoes with insecticides.
Continuous Real-Time Motility Analysis of Acanthamoeba Reveals Sustained Movement in Absence of Nutrients
Authors: Campolo Allison , Harris Valerie , Walters Rhonda , Miller Elise , Patterson Brian , Crary Monica
Published: August 2021

Acanthamoeba keratitis is a serious ocular infection which is challenging to treat and can lead to blindness. While this pathogen is ubiquitous and can contaminate contact lenses after contact with water, its habits remain elusive. Understanding this organism’s natural behavior will better inform us on how Acanthamoeba colonize contact lens care systems. Acanthamoeba trophozoites were allowed to adhere to either a glass coverslip or non-nutrient agar (NNA) within a flow cell with nutrients (Escherichia coli or an axenic culture medium (AC6)) or without nutrients (Ringer’s solution). Images were taken once every 24 s over 12 h and compiled, and videos were analyzed using ImageJ Trackmate software. Acanthamoeba maintained continuous movement for the entire 12 h period. ATCC 50370 had limited differences between conditions and surfaces throughout the experiment. Nutrient differences had a noticeable impact for ATCC 30461, where E. coli resulted in the highest total distance and speed during the early periods of the experiment but had the lowest total distance and speed by 12 h. The Ringer’s and AC6 conditions were the most similar between strains, while Acanthamoeba in the E. coli and NNA conditions demonstrated significant differences between strains (p < 0.05). These results indicate that quantifiable visual tracking of Acanthamoeba may be a novel and robust method for identifying the movement of Acanthamoeba in relation to contact lens care products. The present study indicates that Acanthamoeba can undertake sustained movement for at least 12 h with and without nutrients, on both rough and smooth surfaces, and that different strains have divergent behavior.
The Idea of Landing Sites and Its Inheritance through Tactileology
Authors: Akiba Fuminori
Published: August 2021

From the perspective of sustainability, empowering people to live positively without being dominated by death is an important issue. One thing we can do in this vein is to expand one’s own physical sensation, which is the basis for us to live. From this point of view, Shusaku Arakawa and Madeline Gins’ idea of “landing sites” is very important. Landing sites are physical experiences that result from person–environment collaboration. In order to make as many people as possible aware of their physical sensations through landing sites, Arakawa and Gins created artificial environments such as “Site of Reversible Destiny Yoro” where people could gain new physical sensations. They wanted people to build new ethics and move toward social reformation based on their new physical sensations. However, at present, these artificial environments have some problems. It is the time to seriously consider how we can pass on the experience of landing sites to future generations. The aim of this paper is to provide an answer to the question by Yasuhiro Suzuki’s scientific research on tactile sense, called tactileology. I first introduce Arakawa and Gin’s text about the idea of “landing sites” and make clear its importance. Next, I point out that, now, “landing sites” present certain difficulties. I then confirm that tactileology inherits the idea of “landing sites”.
Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization
Authors: Škarpa Petr , Školníková Marie , Antošovský Jiří , Horký Pavel , Smýkalová Iva , Horáček Jiří , Dostálová Radmila , Kozáková Zdenka
Published: August 2021

Phosphorus (P) is an important nutrient in plant nutrition. Its absorption by plants from the soil is influenced by many factors. Therefore, a foliar application of this nutrient could be utilized for the optimal nutrition state of plants. The premise of the study is that foliar application of phosphorus will increase the yield of normal-phytate (npa) cultivars (CDC Bronco a Cutlass) and low-phytate (lpa) lines (1-2347-144, 1-150-81) grown in soils with low phosphorus supply and affect seed quality depending on the ability of the pea to produce phytate. A graded application of phosphorus (H₃PO₄) in four doses: without P (P0), 27.3 mg P (P1), 54.5 mg P (P2), and 81.8 mg P/pot (P3) realized at the development stages of the 6th true leaf led to a significant increase of chlorophyll contents, and fluorescence parameters of chlorophyll expressing the CO2 assimilation velocity. The P fertilization increased the yield of seeds significantly, except the highest dose of phosphorus (P3) at which the yield of the npa cultivars was reduced. The line 1-2347-144 was the most sensible to the P application when the dose P3 increased the seed production by 42.1%. Only the lpa line 1-150-81 showed a decreased tendency in the phytate content at the stepped application of the P nutrition. Foliar application of phosphorus significantly increased ash material in seed, but did not tend to affect the protein and mineral content of seeds. Only the zinc content in seeds was significantly reduced by foliar application of P in npa and lpa pea genotypes. It is concluded from the present study that foliar phosphorus application could be an effective way to enhance the pea growth in P-deficient condition with a direct effect on seed yield and quality.
Allelopathic and Herbicidal Effects of Crude Extract from Chromolaena odorata (L.) R.M.King and H.Rob. on Echinochloa crus-galli and Amaranthus viridis
Authors: Poonpaiboonpipat Thanatsan , Krumsri Ramida , Kato-Noguchi Hisashi
Published: August 2021

The present study was aimed at investigating the allelopathic effects of a crude extract from Chromolaena odorata (L.) R.M.King and H.Rob. (Siam weed). The effects of 70% crude ethanol extract from the whole plant, leaf, stem, and root on the germination and growth of Echinochloa crus-galli and Amaranthus viridis seedlings were evaluated using Petri-dish tests under laboratory conditions. Crude extracts from the leaf showed the highest inhibitory activity. The leaf extract (OR) was further separated by sequential solvent extraction to provide hexane (HX), ethyl acetate (ET), and butanol (BU) fractions, which were also evaluated using Petri-dish tests. The hexane fraction was significantly the most active; therefore, it was selected for formulation in a concentrated suspension and tested for its herbicidal characteristics. The formulation showed greater early post-emergence than post- and pre-emergence activities, respectively. The physiological mechanism of the formulation was tested against E. crus-galli and showed that chlorophyll a and b and the carotenoid contents of the leaf dramatically decreased when the concentration was increased, suggesting its ability to disrupt the process of photosynthesis. As thiobarbituric acid reactive substances also occurred in the leaf of E. crus-galli, this suggests lipid peroxidation and cell disruption. These results represent the possibility that C. odorata extract contains inhibitory compounds with herbicidal activity and could be used as an early post-emergence herbicide for weed control.
Study of Postharvest Quality and Antioxidant Capacity of Freshly Cut Amaranth after Blue LED Light Treatment
Authors: Jin Siyuan , Ding Zhaoyang , Xie Jing
Published: August 2021

Freshly cut vegetables are susceptible to microbial contamination and oxidation during handling and storage. Hence, light-emitting diode technology can effectively inhibit microbial growth and improve antioxidant enzyme activity. In this paper, the freshly cut amaranth was treated with different intensities of blue light-emitting diode (LED460nm) over 12 days. Chlorophyll content, ascorbic acid content, antioxidant capacity, antioxidant enzymes activity, the changes in microbial count, and sensorial evaluation were measured to analyze the effects of LED treatment on the amaranth. Blue LED460nm light irradiation improved the vital signs of the samples and extended the shelf life by 2–3 days. The AsA–GSH cycle was effectively activated with the irradiation of 30 μmol/(m2·s) blue LED460nm light. According to the results, the LED460nm light could retard the growth of colonies and the main spoilage bacteria, Pseudomonas aeruginosa, of freshly cut amaranth.
Effects of Non-Thermal Plasma Treatment on Seed Germination and Early Growth of Leguminous Plants—A Review
Authors: Šerá Božena , Scholtz Vladimír , Jirešová Jana , Khun Josef , Julák Jaroslav , Šerý Michal
Published: August 2021

The legumes (Fabaceae family) are the second most important agricultural crop, both in terms of harvested area and total production. They are an important source of vegetable proteins and oils for human consumption. Non-thermal plasma (NTP) treatment is a new and effective method in surface microbial inactivation and seed stimulation useable in the agricultural and food industries. This review summarizes current information about characteristics of legume seeds and adult plants after NTP treatment in relation to the seed germination and seedling initial growth, surface microbial decontamination, seed wettability and metabolic activity in different plant growth stages. The information about 19 plant species in relation to the NTP treatment is summarized. Some important plant species as soybean (Glycine max), bean (Phaseolus vulgaris), mung bean (Vigna radiata), black gram (V. mungo), pea (Pisum sativum), lentil (Lens culinaris), peanut (Arachis hypogaea), alfalfa (Medicago sativa), and chickpea (Cicer aruetinum) are discussed. Likevise, some less common plant species i.g. blue lupine (Lupinus angustifolius), Egyptian clover (Trifolium alexandrinum), fenugreek (Trigonella foenum-graecum), and mimosa (Mimosa pudica, M. caesalpiniafolia) are mentioned too. Possible promising trends in the use of plasma as a seed pre-packaging technique, a reduction in phytotoxic diseases transmitted by seeds and the effect on reducing dormancy of hard seeds are also pointed out.
Intraspecific Genetic Diversity of Cistus creticus L. and Evolutionary Relationships to Cistus albidus L. (Cistaceae): Meeting of the Generations?
Authors: Lukas Brigitte , Jovanovic Dijana , Schmiderer Corinna , Kostas Stefanos , Kanellis Angelos , Gómez Navarro José , Aytaç Zehra , Koç Ali , Sözen Emel , Novak Johannes
Published: August 2021

Cistus (Cistaceae) comprises a number of white- and purple-flowering shrub species widely distributed in the Mediterranean basin. Within genus Cistus, many taxa are subject to various taxonomic uncertainties. Cistus creticus, a prominent member of the purple-flowered clade, is a prime case of the current taxonomic troubles. Floras and databases approve different species names and utilise different or additional/fewer synonyms. Various intraspecific classification systems based on subspecies or varieties are in use. The inconsistent determination of plant material makes it difficult to compare literature regarding the phytochemical diversity and biological activities of plant material and impedes a systematic utilization of the manifold medicinal properties of C. creticus. In the present investigation, we used DNA sequence data from one nuclear region (ITS) and two chloroplast regions (trnL-trnF, rpl32-trnL) to test the intraspecific genetic diversity of C. creticus and its evolutionary relationships to the closely related C. albidus. The combined DNA data confirmed C. creticus as a rather heterogeneous species that integrates two major evolutionary lineages with clearly different genetic characteristics. The ‘Eastern Mediterranean clade’ seems to represent old and ancestral characteristics. This lineage exhibits a close relationship to the geographically distant C. albidus, expressed by very closely related ribotypes and an interspecifically shared chlorotype. The ‘Western Mediterranean clade’ is characterized by a distinctive ITS polymorphism (co-occurring paralogous ribotypes) and more distantly related chlorotypes. The formation of the genetically complex ‘Western Mediterranean clade’ seems to have involved hybridization and recurrent formation or migration movements.
Viscoelastic Behavior of Drug-Loaded Polyurethane
Authors: Abbasnezhad Navideh , Shirinbayan Mohammadali , Chabi Fatiha , Champmartin Stephane , Tcharkhtchi Abbas , Bakir Farid
Published: August 2021

Drug-eluting stents are desirable platforms for local medicine delivery. However, the incorporation of drugs into polymers can influence the mechanical and physicochemical properties of said matrix, which is a topic that is still poorly understood. In fact, this is more noticeable since the apposition is most often accompanied by mechanical stresses on the polymer coating, which can induce therapeutic failure that can result in death. It is therefore necessary to better understand their behavior by examining their properties in conditions such as those in living beings. We studied polyurethane drug carriers made in-house. Diclofenac epolamine was chosen as a model hydrophilic medicine. We used thermal measurements (DMTA) and tensile tests. The aim was to establish the influence of the loading and release of the drug on the physicochemical properties of this polymer in the presence of a stagnant or circulating fluid medium, phosphate-buffered saline (PBS). For the two PU/drug loadings studied, the effect of the initial drug load was more marked. The free volume fraction and the number of pores in the samples increased with the increasing percent of the drug and with release time. The kinetic profiles were accelerated with the loading ratio and with the presence of flow. Young′s modulus and ultimate stress were not significantly influenced by the release time. A relevant relationship between the tensile properties and the viscoelastic behavior of the samples was developed. Our results have implications for optimizing the performance of drug coatings for stents.
Environmentally Friendly UV-Protective Polyacrylate/TiO2 Nanocoatings
Authors: Zeljko Martina , Ocelić Bulatović Vesna , Špada Vedrana , Blagojević Sanja Lučić
Published: August 2021

The development of coatings that maintain the attractive natural appearance of wood while providing ultraviolet (UV) protection is extremely important for the widespread use of wood products. In this study, the influence of different types (powder form and aqueous dispersions) of TiO2 in an amount of 1.0 wt% by monomer weight on the properties of environmentally friendly polyacrylate (PA)/TiO2 emulsions prepared by ex situ and in situ polymerization, as well as on the UV-protective properties of the coating films, was investigated. The results showed that the addition of TiO2 significantly affected the particle size distribution of PA and the viscosity of PA varied according to the preparation method. Compared with the ex situ preparation method, in situ polymerization provides better dispersibility of TiO2 nanoparticles in PA coating film, as well as a better UV protection effect and greater transparency of the coating films. Better morphology and transparency of nanocoating films were achieved by adding TiO2 nanofillers in aqueous dispersion as compared to the addition of TiO2 in powder form. An increase in the glass transition temperature during UV exposure associated with cross-linking in the polymer was less pronounced in the in situ-prepared coating films, confirming better UV protection, while the photocatalytic effect of TiO2 was more pronounced in the ex situ-prepared coating films. The results indicate that the method of preparation has a significant influence on the properties of the coating films.