bluuebunny/crossref_metadata_2025 huggingface dataset:
A light microscopic study of placentae in eclamptic mothers at term
Authors: Meyur Rudradev , Sadhu Anirban , Mondal Hironmoy , Khanra Santanu Kumar
Published: August 2017

Background: Eclampsia is one of the leading causes of maternal and consequent foetal mortality in India. Studies on the histology of eclampsia are relatively uncommon, more so in the eastern region of India. The present study therefore is an attempt to analyse the changes which occur in placentae of eclamptic mothers with the help of light microscope as it is considered to be the epicentre of the disease. These findings are useful to understand the pathology of the killer disease and improve morbidity and mortality.Methods: 60 cases were selected for the study. 30 were non-eclamptic and 30 were eclamptic. Abnormalities in blood vessels, villi and basement membrane of placentae were searched for after routine H and E staining.Results: Eclamptic cases recorded with higher incidences of fibrinoid necrosis of villi, cytotrophoblastic proliferation, increased syncytial knots, hypo vascular villi and thickening of basement membrane. Non-eclamptic cases recorded only fibrinoid necrosis. Other features were not observed in the latter cases.Conclusions: Light microscopic changes observed in eclamptic placentae may contribute to the pathogenesis of the condition and serve as the baseline for further studies.
Yield of different bronchoscopic techniques in diagnosis of lung cancer
Authors: Kumar Vipul , Gupta K. B. , Aggarwal Ritu
Published: August 2017

Background: Lung cancer is generally diagnosed during late stage of the disease so early diagnosis of lung cancer is very important to reduce lung cancer death rate. Flexible fibreoptic bronchoscopy revolutionized early diagnosis of lung cancer as it provides sufficient cytologic and histologic specimens in form of bronchial brushings, broncho-alveolar lavage and bronchial forceps biopsy. Cytologic techniques are safe, economical, and provide quick results. They not only complement tissue biopsies in the diagnosis of lung cancer but are also comparable in yield.Methods: The present study analyzes cytology of Bronchoalveolar lavage, Bronchial Brushings and histology of bronchial biopsy in 45 patients diagnosed as lung cancer by fiber-optic bronchoscopy. Age, gender, smoking habits, various histological types of malignancies, and yield of various bronchoscopic diagnostic techniques in the diagnosis of lung cancer were evaluated.Results: Of the 45 cases with confirmed diagnosis, 37 (82.22%) were males and 8 (17.17%) were females with male to female ratio of 4.6:1. The mean age in this study group was 54.71 years. Squamous cell carcinoma was the most common primary bronchogenic tumor (62.22%) followed by adeno carcinoma (26.66%). The overall diagnostic yield of fiber-optic bronchoscopy procedures was 100% (32/32 patients) in bronchoscopically visible tumors. Bronchial biopsy was most sensitive (100%) followed by Bronchial Brushings (88%) and BAL (81.25%). However, in non-visible tumors, biopsy, brush and BAL yielded diagnostic specimens for lung cancer in 84.61%, 76.92% and 46.15% of patients respectively.Conclusions: Lung cancer is a common malignancy with male preponderance. Bronchial biopsy has a very high diagnostic yield. Cytopathological examination of bronchial brushings and broncho-alveolar lavage not only complement tissue biopsies in the diagnosis of lung cancer but have comparable diagnostic yield.
Implications of inter observer variability in cervical smear reporting
Authors: Sushma . , Jacob Roshny
Published: August 2017

Background: In spite of the Bethesda system 2001 (TBS 2001), formulating strict guidelines for reporting cervical smears, intra observer and inter observer variations are unavoidable and can be considered an inherent part of the reporting system. The implications of this variation are in the quality of performance of the reporting laboratory and in the patient management. Rescreening is a tool to reduce the variations and improve the quality of both the laboratory staff and laboratory as such. Rescreening by two or more experienced observers has helped in identifying new cases better. The present study aims to rescreen cervical smears by two independent observers, to compare the results of the two independent observers and to understand the implications of this variability on the quality of cervical smear reporting.Methods: 1000 consecutive cervical smears were rescreened by two experienced cyto-pathologists independently. Their findings were charted out and analyzed statistically for kappa value.Results: Initial reporting had identified 20 cases of neoplastic nature. First observer identified, in addition, 6 new cases and second observer identified 12 new cases. The inter observer variability of 6 cases showed a kappa value of 0.89.Conclusions: Rescreening is a safe way of picking up missed cases. Rescreening by two or more observers is better in identifying new cases. This helps in improving the quality of reporting personnel and the laboratory as well as in improving patient care.
OCT based evaluation of retinal changes in multiple sclerosis
Authors: Singh Sonal , Sharma Rishi , Gurunadh V. S. , Shankar Sandeep
Published: August 2017

Background: Optical coherence tomography is a non-invasive imaging technique routinely used in ophthalmology to visualize and quantify the layers of the retina. It also provides information on optic nerve head topography, peripapillary retinal nerve fibre layer thickness and macular volume which correlate with axonal loss. These measurements are of interest in optic neuropathies and in multiple sclerosis. The OCT parameters are now used as endpoints in neurologic clinical trials.Methods: A prospective study involving 30 patients of multiple sclerosis and equal number of age and sex matched controls were subjected to evaluation of retinal changes (peripapillary retinal nerve fiber layer and central macular thickness) using Zeiss Cirrus HD-OCT machine. The results collected were then subjected to statistical analysis.Results: Significant RNFL thinning was seen in patients of multiple sclerosis compared to the age and sex matched controls. Marked thinning was seen in superior and temporal quadrants of right eye (p value of 0.002 and 0.008 respectively) and all quadrants in left eye with a p value of < 0.001. Patients with multiple sclerosis for more than 5 yrs showed statistically significant RNFL thinning in the superior quadrant of right eye (p<.005), however, no such changes were seen in rest of the quadrants of right eye and in none of the quadrants of left eye Significant RNFL thinning was seen in the patients of multiple sclerosis without prior history of optic neuritis than patients with prior history of optic neuritis which was statistically significant with p value of .001.Conclusions: Patients with multiple sclerosis for more than 5 yrs showed statistically significant RNFL thinning in the superior quadrant of right eye (p<.005). Significant RNFL thinning was seen in the patients of multiple sclerosis without prior history of optic neuritis than patients with prior history of Optic neuritis which was statistically significant with p value of .001. No significant changes were seen in central macular thickness in multiple sclerosis compared to the controls which was corroborated by statistical analysis (p value of 0.37).
Study of metabolic changes-glycoprotein and phospholipids levels in patients of malaria
Authors: Tyagi Rupal A. , Tyagi Amit G. , Choudhary Prema Ram , Shekhawat Jaidev Singh
Published: August 2017

Background: In erythrocytic stage, malarial parasites meet their high glucose requirement only by modulating the host cell membrane by increasing transport of sugar across the host cell membrane. This leads to a transmembrane gradient of the substrate and finally leading to alterations of metabolic changes and permeability of RBC membrane. Therefore, the aim of present study was to determine the parameters which reflect the status of RBC membrane and their association with the severity of malaria in a large cohort of known patients of malaria, which was caused by the Plasmodium Species.Methods: Blood sample were collected in EDTA bulb at the time of admission (day-1) and on third day (day-3). The samples were analyzed within 24 hours of collection. Erythrocytic total phospholipid is measured by modified connerty method, Total sialic acid (TSA) is measured by TBA/dimethyl sulphoxide method.Results: The mean levels of erythrocytic phospholipid, plasma TSA and PBSA in the cases of malaria were significantly increase (P<0.001) as compared to those in the control group. In the follow up study the same parameters were studied in patients post anti-malarial treatment day-3. The level of erythrocyte phospholipid, plasma TSA and PBSA were reversed.Conclusions: On the basis of the present study it is suggested that the anti-malarial drug regimen must be supported by antioxidants and trace elements supplementation to improve the status of deviated biochemical parameters towards normalcy.
Assessment of innovations in tutorial method of teaching among medical students
Authors: A. Velavan , V Saravanan , Bazroy Joy , Singh Zile
Published: August 2017

Background: The main focus of a tutorial is to make learning more interactive. Enthusiasm and innovations of tutors make tutorials more effective. This study was done to assess the usefulness of few innovative approaches applied during a tutorial session among medical students.Methods: A total of 84 medical undergraduate students were divided in to seven groups with 12 students in each group with a faculty as a facilitator. A pretest was conducted among the students to assess their knowledge and understanding on the topic of discussion. Then the students were given a review article on the topic of discussion to read followed by group discussion. After this each group was assessed by the way of a quiz programme and individually by a post test.Results: The mean scores obtained in the pretest increased significantly in the post test from 53.6% to 83.7%. Most of the students agreed upon the various advantages of this method of teaching. About 97% of the students rated this method of teaching as good and above.Conclusions: Tutorials are effective methods of teaching medical graduates. The innovations used in this study were well appreciated by the students.
Temporary cardiac pacing induced electrocardiographic changes simulating myocardial infarction
Authors: Behera Suresh Kumar , Samal Akshaya Kumar , Pradhan Akshyaya Kumar
Published: August 2017

Temporary transvenous pacing is an immediate lifesaving measure in patients with Stokes-Adams syndrome and in patients with symptomatic bradycardia. Bradyarrhythmias are known to occur in acute myocardial infarction. But in a paced heart, it is difficult to diagnose myocardial infarction from electrocardiogram (ECG) because pacemaker rhythm causes distortion of natural wave forms. On the other hand, remarkable T wave inversions and ST depressions do occur in the ventricular paced ECG as secondary changes. The case report describes a patient who developed profound de novo T wave inversions and ST depressions in the unpaced ECG following temporary transvenous pacing simulating MI.
Paraplegia in young female after stab injury: a rare injury with heavy socioeconomic burden and its impact
Authors: Wandile Kiran Narayanrao , Mote Girish Balasaheb , Badole Chandrashekhar Martand
Published: August 2017

Thoracic spinal cord stab injuries are rare lesions. We report a 32 years old young married female, of a lower middle class, who was stabbed on her back with a sickle by her neighbor after a quarrel. She presented with complete paraplegia with muscle power of zero on all muscle groups, complete sensory loss from thoracic dermatome level 4 and below, acute urinary retention, and a 3-centimeter vertically placed wound on the posterior thoracic region from which cerebrospinal fluid mixed with blood was oozing out. A high-dose methylprednisolone protocol was started (30 mg/kg in one hour and then 5.4 mg/kg over next 23 hours), urinary catheter placed and sterile cleaning and dressing was done. Antibiotics and analgesics were also administered. The Magnetic Resonance Imaging scan was done urgently and scanning revealed thoracic spinal cord contusion at D5 vertebral level with cord oedema at D4 to D6 vertebral level and fracture spinous process of T4 vertebral body. The case is managed conservatively and she is under follow up. As patient is a young married female, wedge worker by occupation, having lower middle class of socioeconomic status, this condition has high impact considering the socioeconomic issues.
Anesthetic management of a patient with situs inversus posted for laparoscopic cholecystectomy
Authors: Hazarika Plabon , Das Prabir Pranjal
Published: August 2017

Situs inversus totalis is a rare congenital visceral malrotation anomaly that results from disturbances in establishment of left-right asymmetry. It is an autosomal recessive condition, in which organs are transposed from their normal location to the opposite side of the body and the predicted incidence is one in 10, 000 among the general population. In a patient with situs inversus totalis, not just the diagnosis of any acute abdomen pathology is difficult but equally challenging is the anesthetic management during the respective surgical procedure. We are reporting a patient who had situs inversus totalis and was operated for laparoscopic cholecystectomy under general anesthesia, and endotracheal tube as an airway conduit. Though the problems related to such patients are mainly of surgical feasibility, an anesthesiologist must be aware of the associated problems of both, situs inversus and the surgical procedures. The present case report lays an emphasis on the potential difficulties during anesthetic management and its various implications in a remote area in North East India with resource limitations. To the best of our knowledge, we report the first case from a remote are in North East India of a successful laparoscopic cholecystectomy in a patient with situs inversus totalis under general anesthesia which was uneventful.
Vasoplegic syndrome after off pump coronary artery bypass grafting surgery
Authors: Vaidyanathan Swaminathan , Chidambaram Kumar , Mohanraj Anbarasu
Published: August 2017

We report a case of 48-years-old man who developed nor-epinephrine resistant vasoplegic syndrome after elective off pump coronary artery bypass grafting surgery. The failure of norepinephrine to improve the patients’ hemodynamics promted us to start treatment with vasopressin. Within an hour, the hemodynamics begin to improve. After 4 hours, patient was stable enough to be weaned from vasopressin, extubated at the end of 24 hrs and shifted to ward at the end of 48 hours. Vasoplegic syndrome can occur commonly after cardiopulmonary bypass and incidence can go up to 10%. This case was reported to insist upon the fact that vasoplegic syndrome can occur even after off pump surgeries. Herein we discuss the aetiology, risk factors, pathophysiology and prophylactic measures to be taken, for the prevention of vasoplegic syndrome.
Análise da influência do horário do dia e da época do ano em imagens fotográficas para identificação de manifestações patológicas em fachadas na cidade de Pelotas/RS
Authors: Rodrigues Aretusa Oliveira , Paliga Charlei Marcelo , Torres Ariela Da Silva
Published: August 2017

A preocupação com os problemas patológicos em edificações é muito antiga, podendo, estes danos, aparecerem em qualquer elemento da construção. Um dos componentes mais propícios a possuírem problemas patológicos é o revestimento de fachada, devido a sua exposição às intempéries. Porém, apesar dessa preocupação, não existem procedimentos normatizados para analisar as manifestações patológicas em edificações. Todavia, há um consenso que os subsídios levantados na coleta de dados são fundamentais para a formulação do diagnóstico e a definição da conduta. Baseado neste contexto, o objetivo principal deste trabalho foi analisar a influência de variáveis em imagens fotográficas para identificação de manifestações patológicas em fachadas argamassadas na cidade de Pelotas/RS. Foram realizadas observações dos danos de fissura, descolamento e mancha de umidade com presença de microrganismos em fachadas sudeste e noroeste, analisando a influência dos horários do dia e das estações do ano. Como resultados, verificou-se a influência do horário do dia e da época do ano no dano de mancha de umidade com presença de microrganismo, sendo considerado o horário das 12:00 horas e estação da primavera como os que permitiram mais fácil visualização do referente dano nas imagens fotográficas; no problema patológico de fissura houve influência somente do horário do dia, onde percebeu-se que quando não há projeção dos raios solares o mesmo torna-se de mais fácil visualização nas fotografias; e, na manifestação patológica de descolamento não houve influência dessas variáveis.
Engaging Pre-Service Teachers in the Drama in Teacher Leadership
Authors: Cranston Dr. Jerome , Kusanovich Kristin
Published: August 2017

This paper presents the findings of a qualitative research study that examined the effects of a transdisciplinary ethnotheatre workshop designed to support the professional development of school leaders as they navigate the complexities of teacher leadership. The site of inquiry was a pre-service teacher leadership workshop held in a graduate school class where participants analyzed, witnessed, and enacted ethnodramas, problematizing tensions in teacher leadership. Using a constant comparative approach, the participant journals were read and re-read, coded, and then categorized thematically with particular focus on negative case analysis. The authors present excerpts from the ethnodrama scripts used in the workshop alongside the research findings, which suggest that, while some participants perceived the injustice of a given situation presented in the ethnodrama, and could articulate how the process of ethnotheatre created avenues for learning about the lived reality of teacher leaders, others indicated either a lack of awareness or a tacit acceptance of the bullying and discriminatory behaviors embedded within the scripts.
Arts-based Methods in Socially Engaged Research Practice: A Classification Framework
Authors: Wang Qingchun , Coemans Sara , Siegesmund Richard , Hannes Karin
Published: August 2017

Arts-based research has recently gained an increasing popularity within qualitative inquiry. It is applied in various disciplines, including health, psychology, education, and anthropology. Arts-based research uses artistic forms and expressions to explore, understand, represent, and even challenge human experiences. In this paper we aim to create order in the messy field of artistically inspired methods of socially engaged research. We review literature to establish study and distinguished three major categories for classifying arts-based research: research about art, art as research, and art in research. We further identify five main forms of arts-based research: visual art, sound art, literary art, performing art, and new media. Relevant examples of socially engaged research are provided to illustrate how different artistic methods are used within the forms identified. This classification framework provides artists and researchers a general introduction to arts-based research and helps them to better position themselves and their projects in a field in full development.
Reviews Editorial: Introduction to Snapshot Reviews
Authors: Prendergast Monica
Published: August 2017

An introduction to the "snapshot" reviews in this issue of Art/Research International from Section Editor Monica Prendergast.
GOVERNO DOS ALGORITMOS
Authors: Silveira Sergio Amadeu
Published: August 2017

Este texto trata da governamentalidade dos algoritmos no setor público. Debate as noções de governo, governança e governamentalidade. A partir dessas definições mostrar o nível de penetração dos algoritmos em nossa sociedade e indica a tendência de expansão de seu uso nas instituições do Estado. Por fim, analisa as implicações dos algoritmos no setor público e os desafios de mantê-los sob controle de uma gestão democrática e socialmente inclusiva, superando as perspectivas tecnocráticas, positivistas e neoliberais.
A INDÚSTRIA PARANAENSE E O PROGRAMA PARANÁ COMPETITIVO: DESCENTRALIZAÇÃO E O DESENVOLVIMENTO DO SETOR
Authors: Pelinski Raiher Augusta , Souza do Carmo Alex Sander , Yukio Higachi Hermes , Wekerlin Jorge Eduardo
Published: August 2017

O objetivo deste trabalho é analisar a descentralização e o desenvolvimento da indústria entre os municípios paranaenses, investigando a importância do Programa Paraná Competitivo neste processo. Para isso, construiu-se um índice de desenvolvimento da indústria (IDI) e por meio da econometria espacial, analisou-se o efeito do Programa Paraná Competitivo na descentralização e no desenvolvimento da indústria entre os municípios paranaenses. Verificou-se um processo de descentralização da indústria ao longo do estado, com o melhoramento da sua produtividade, apresentando um efeito positivo e significativo do Programa tanto no processo de descentralização como também no desenvolvimento do setor
ANÁLISE DA EVOLUÇÃO DO IMPOSTO SOBRE SERVIÇOS NA ARRECADAÇÃO TRIBUTÁRIA NO MUNICÍPIO DE CAMPINAS-SP
Authors: Cappa Josmar , Filho José Vicente de Souza
Published: August 2017

O artigo analisa a importância do Aeroporto Internacional de Viracopos paraa arrecadação tributária do município de Campinas-SP. Para isso, foi feita discussãoteórica sobre o papel estratégico de grandes aeroportos na dinâmica daeconomia contemporânea e reconstituído o debate sobre a estrutura tributáriabrasileira para destacar, a partir da Constituição Federal de 1988, a descentralizaçãotributária para estados e municípios e a elaboração dos orçamentos públicosno país. A análise constata que a evolução da arrecadação tributária emCampinas foi recuperada entre 1995 e 2013 a partir de informações primáriasda Secretaria Municipal de Finanças. Concluiu-se que Viracopos foi importanteno desempenho do ISS, apesar de não ser exclusivo, e que estamos diante denova tendência na arrecadação tributária, tornando-se necessária a instituiçãode políticas públicas de gestão aeroportuária.Palavras-chave: Políticas Públicas, desenvolvimento local, transporte aéreo,aeroporto.
Emociones e identidad profesional docente: relaciones e incidencia
Authors: Buitrago-Bonilla Rafael Enrique , Cárdenas-Soler Ruth Nayibe
Published: August 2017

La Identidad Profesional Docente (IPF) está íntima y continuamente ligada a las emociones desde el mismo momento en que se estructura el perfil de identidad y en cada transformación que se da. Los estudios que abordan la identidad están pasando de plantear cuestiones respecto a quién se es para asumir nuevas perspectivas orientadas a cuándo, dónde y cómo se es. En este marco conceptual, el presente artículo presenta resultados concernientes a los aspectos relacionados con la identidad y las emociones que diecisiete estudiantes de últimos semestres de los programas de formación de maestros de la Universidad Pedagógica y Tecnológica de Colombia (UPTC) atribuyen a la profesión que han escogido. La investigación se valió del estudio de caso, con múltiples unidades de análisis, y de la entrevista a profundidad como técnica para la recolección de los datos, los cuales se analizaron con el apoyo del programa informático ATLAS TI. En conclusión, las identidades son diversas, dinámicas y complejas, y se configuran a partir de componentes intrapersonales, vivencias emocionales y experiencias en la práctica de aula, en correspondencia con las características del contexto.
¿Sirve la historia de la educación hoy?
Authors: Pulido Cortés Oscar
Published: August 2017

La pregunta por la historia de la educación, como campo investigativo o disciplina, revive cada vez que existen cuestionamientos y reformas curriculares que involucran las formas como se materializa la formación de profesores en las actuales circunstancias históricas, que exigen cambios y transformaciones. La historia de la educación se convierte en una posibilidad de retornar con perspectivas contemporáneas a las formas primigenias y posibilitadoras de los discursos y las prácticas pedagógicas; asimismo, permite a las nuevas generaciones mostrar cómo se configuró lo que hoy entendemos como lo educativo y pedagógico. De igual forma, esta mirada a las condiciones de posibilidad de proyectos formativos, instituciones y formas de enseñanza y aprendizaje produce un particular interés por comprender los contextos y las posibilidades de lo educativo y pedagógico en ámbitos de incertidumbre y cambio veloz en todos los sectores.
Editorial English
Authors: Santos Gildenir Carolino , Passos Rosemary
Published: August 2017

We are pleased to bring to you this issue, volume 10 - number 1, with unpublished works of researchers and authors of various areas, that once again contribute with ETD - Digital Thematic Education, and therefore, with the whole area of education and related fields.
Jogando com o narrador: estratégias narrativas na produção de textos em ambientes escolares informatizados
Authors: Mungioli Maria Cristina Palma
Published: August 2017

Este relato apresenta a análise de algumas estratégias narrativas empregadas por alunos de 5a. e 6a. séries do Ensino Fundamental na produção de textos em ambiente informatizado. O foco de atenção recai sobre as estratégias usadas pelo narrador na construção textual. As narrativas analisadas foram elaboradas por meio de um processador de textos e de imagens (Micromundos). A perspectiva de análise adotada considera tanto as pesquisas acerca da narrativa, entendida como forma de compreensão, representação e criação da realidade quanto questões relativas à dialogia e à interação verbal. Abordam-se também alguns aspectos referentes ao uso do computador em ambiente escolar. Discutem-se algumas das transformações ocorridas com a chegada do computador na sala de aula no que diz respeito ao relacionamento aluno-aluno, aluno-computador, aluno-professor e aluno-conhecimento.
Efeitos da ausência paterna na aprendizagem
Authors: Monticelli Fernanda Ferreyro
Published: August 2017

O texto parte de uma situação de ensino envolvendo gênero dos substantivos como modo de abordar outra compreensão do erro no processo de aprendizagem. Sugere como abordagem as significações imaginárias presentes na sociedade ao verificar como a resposta do aluno pode indicar para um ponto significativo na estrutura do sujeito que no caso apresentado poderia ter relação com a ausência paterna. Após, com a intenção de ampliar a discussão sobre as conseqüências da ausência do pai é apresentado uma síntese das dissertações e teses cadastradas no CAPES cuja categoria envolve paternidade.
Reflexões contemporâneas para as altas habilidades/superdotação
Authors: Delpretto Bárbara Martins de Lima , Freitas Soraia Napoleão
Published: August 2017

Cientes das transformações tecnológicas e científicas da atualidade sob influência da globalização, investigações científicas quanto à temática das altas habilidades/superdotação se mostram necessárias e essenciais, em uma ênfase a diversidade. O presente estudo tem por objetivo evidenciar, desta forma, reflexões contemporâneas com bases em pesquisas na área, de modo a estabelecer uma relação entre algumas das prioridades no conhecimento formal e as metas a ampliação de oportunidades para tais sujeitos.
Filarmônica Nossa Senhora da Conceição (Itabaiana-SE): estudo musicológico-histórico sobre a origem dos seus antecedentes
Authors: Moreira Marcos
Published: August 2017

Este artigo pretende fazer uma abordagem o processo de ensino da Filarmônica N.S. da Conceição de Itabaiana, município que se localiza a 60 km de Aracaju, Estado de Sergipe. O objetivo do tema procede para que possamos entender e refletir sobre educação musical em Sergipe através da história da Instituição.
The Extracellular Domain of Human High Affinity Copper Transporter (hNdCTR1), Synthesized by E. coli Cells, Chelates Silver and Copper Ions in Vivo
Authors: Sankova Tatiana P. , Orlov Iurii A. , Saveliev Andrey N. , Kirilenko Demid A. , Babich Polina S. , Brunkov Pavel N. , Puchkova Ludmila V.
Published: August 2017

There is much interest in effective copper chelators to correct copper dyshomeostasis in neurodegenerative and oncological diseases. In this study, a recombinant fusion protein for expression in E. coli cells was constructed from glutathione-S-transferase (GST) and the N-terminal domain (ectodomain) of human high affinity copper transporter CTR1 (hNdCTR1), which has three metal-bound motifs. Several biological properties of the GST-hNdCTR1 fusion protein were assessed. It was demonstrated that in cells, the protein was prone to oligomerization, formed inclusion bodies and displayed no toxicity. Treatment of E. coli cells with copper and silver ions reduced cell viability in a dose- and time-dependent manner. Cells expressing GST-hNdCTR1 protein demonstrated resistance to the metal treatments. These cells accumulated silver ions and formed nanoparticles that contained AgCl and Ag0. In this bacterial population, filamentous bacteria with length about 10 μm were often observed. The possibility for the fusion protein carrying extracellular metal binding motifs to integrate into the cell’s copper metabolism and its chelating properties are discussed.
GATS and International Trade in Health Services: Impact and Regulations
Authors: Driss Bakhouya
Published: August 2017

This article is interested in studying the impact of trade agreements on the quality of health services, and the statement of the role of the Ministry of Health to protect the quality of these services. The problems that arise in this study; how the application of GATT on trade in health, and services? This article also includes the impact of international trade agreements on the issue of attracting foreign investments in the field of health, and to clarify the government's ability to maintain the health and political, and the capacity of national health products to compete with foreign products. As pointed out repeatedly in this paper, it is too early to make definitive conclusions regarding the impact of GATS, or the liberalization of trade, in the health sector. Nevertheless, we conclude that although so far only a few countries where trade in health services has a major role, trading as it does –which is not based on the GATS or GATS commitments– and appears to be increasing, GATS agreement provides a framework for international trade in services, including health services, but the actual content and obligations are determined in large part by the national government.
Studi Tempat Bertengger Burung Cikalang di Suaka Margasatwa Pulau Rambut
Authors: Wardhani Putri Kesuma , Sukandar Paskal , Isfaeni Hanum
Published: August 2017

Abstract

Frigatebird is one of seabird species found in Pulau Rambut Wildlife Reserve (SMPR). The bird activities include roost in fish pole, flying, foraging in the ocean and also roosting in SMPR at night. The aim of this research was to know roosting site used by frigatebird while at SMPR. This research was conducted in SMPR, Jakarta on August and September 2010 by descriptive method and survey techniques. Data was collected directly on the object of research. Frigatebird population was calculated directly by counting individuals one by one as they flew low in the air over SMPR. Frigatebird roosting site was also researched directly by observing frigatebird when going to roost. Furthermore, vegetation analysis was also conducted on trees in roosting site. Based on our obsevations, the population of frigatebird obtained in SMPR ranged between 87-193 birds. Frigatebird utilize Sterculia foetida tree to be used as a place to roost. This roosting site located in mixed secondary forest to the west and south SMPR close to mangrove forest. Average of height and diameter of S. foetida tree used to roost is 23.47 m and 42.26 cm. There were commonly 3 species of tree of frigatebird roosting site namely Ficus sp. (INP 16.91%), Allophylus cobbe (INP 16.78%) dan Diospyros maritima (INP 14.23).

 

Key words: frigatebird, Pulau Rambut Wildlife Reserve (SMPR), roosting site
Keragaman Karakter Morfologi, Komponen Hasil, dan Hasil Plasma Nutfah Kedelai (Glycine max L.)
Authors: Putri Priskilla Purnaning , Adisyahputra Adisyahputra , Asadi Asadi
Published: August 2017

Abstract

Soybean (Glycine max L.) is annual crop that have high morphologies and yield components diversity. The research was conducted at the first season of 2011, the objective of the research were to find morphological, yield, and yield component of Soybean germplasm (Glicine max L.). The research was carried out at experimental station BB-BIOGEN Citayam, Depok, and laboratory of Gene Bank BB-BIOGEN. The experiment used randomized block design with 100 different accessions and three replications for each accession. Based on the observation, the morphological characters have many visual forms. They are as follows: growth percentage in which 19.33 – 99%; growth types were determinate and indeterminate, the leave form was triangle to sharp; purple and white flowers; yellow and black seeds color. The range of values for each characteristic component are as follows: plant height 29,23 – 104,25 cm; number of pods per plant was 23,6 – 99,82; flowering time 33 – 47 days after planting; 100 seed weight 5,98 – 20,77 gram; maturing time 75 – 96,67 days after planting; root nodule’s weight 0,004 – 0,109 gram; seed’s weight 3,15 – 11,45 gram/plant. Among the accessions, the highest yield was shown by B 4323 (643,27 gram/3,6 m2). Significant correlation was shown between soybean’s yield components and yield which were plant’s height, growth percentage, numbers of main stem’s node, numbers of pods, seeds weight for each plant and root nodule’s weight. 100 seeds weight showed significant negative correlation with soybean components.

 

Key words: germplasm, morphological characteristics, soybean, yield components
DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA
Authors: Rachmawati Rezki , Muzajjanah Muzajjanah , Rustam Yoswita
Published: August 2017

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.
PERBANDINGAN PROFIL LIPID PADA SERUM DARAH PENDERITA HIPERTENSI DAN NORMOTENSI
Authors: Widyamurti Pratiwi , Rusdi Rusdi , Rahayu Sri
Published: August 2017

ABSTRACT

Increased blood pressure more than 140/90 mmHg taken from three measurement in 24 hours can be diagnosed as hypertension. Abnormality of lipid values condition was found at many hypertensive. Based on this reason examination of lipid profile in hypertensive and normotensive should be done. The aim of this research was to measure and compare lipid profile on blood serum in hypertensive    and normotensive. Lipid profile was measured by Konelab 20XT clinical chemistry analyzer. Ex     Post Facto used as method and Cross-sectional used as design. A total of 50 blood samples collected from Hypertensive (N1=25) and normotensive (N2=25) from June to August 2014. SPSS 16.0 was used to analyze the data, T-test was used to compare value of LDL cholesterol, HDL cholesterol and total cholesterol while U Mann-Whitney test was used to compare value of triglyceride. The result      of this research showed that the mean value of triglyceride was 146.56 mg/dL in hypertensive and

143.92 mg/dL in normotensive (p=0.11). The mean value of LDL cholesterol was 129.80 mg/dL in hypertensive and 136.72 mg/dL in normotensive (p=0.62). The mean value of HDL cholesterol was  38.80 mg/dL in hypertensive and 45.04 mg/dL in normotensive (p=0.1). The mean value of total cholesterol was 201.04 mg/dL in hypertensive and 221.88 mg/dL in normotensive (p=0.25). In conclusion, there was no different of lipid profile on blood serum in hypertensive and normotensive.

 Keywords: hypertension, lipid profile, normotensive
IDENTIFIKASI FRAGMEN DNA MIOGLOBIN SEPANJANG 114PB PADA BEBERAPA JENIS HEWAN LAUT YANG MAMPU HIDUP PADA ZONA MINIMUM OKSIGEN
Authors: Febrianti Isrina , Puspitaningrum Rini , Rusdi Rusdi
Published: August 2017

ABSTRACT

Myoglobin is a hemoprotein that contributes to intracellular oxygen storage and facilitates diffusion oxygen into mitochondria. Myoglobin production will increase as low levels of environmental oxygen called hypoxia. When oxygen in tissue is low, the body will respond by forming myoglobin in some tissues, such as muscle tissue. The aims of this study was to detect DNA fragments of myoglobin along 114pb in muscle tissue of marine animals are capable to live in the oxygen minimum zone, such as the green turtle, mackerel tuna and hammerhead sharks. The oxygen minimum zone is zone of oceans which have low dissolved oxygen levels at > 1,4μLO2L-1, the oxygen levels can be categorized as hypoxic conditions. This study was conducted in the Biochemistry laboratory, State University of Jakarta. This study used descriptive method by PCR and electrophoresis. The results indicated that myoglobin found in muscles of marine animals were capable live in oxygen minimum zone such as the green turtle, mackerel tuna and hammerhead shark. This information can be used as the basic that the tissue able to make adaptation strategy towards low oxygen environmental levels by increasing formation of myoglobin in muscle tissue.

 Kata kunci: Myoglobin DNA, Green Turtle, Mackerel Tuna and Hammerhead Sharks
INDUKSI MUTASI PADA PISANG (Musa sp. - ABB) cv. KEPOK DENGAN IRADIASI GAMMA SECARA IN VITRO
Authors: Masykuroh Luthfia , Adisyahputra Adisyahputra , Indrayanti Reni
Published: August 2017

Banana (Musa sp. - ABB) cv. Kepok is one of type banana processed that have a very potential commodities fruit developed to support food survival. The purpose of this study was to knowing the effect of gamma irradiation on the growth of banana plants cv. Kepok in vitro. This study was conducted in October 2014 – October 2015 in Plant Tissue Culture Laboratory, Biological – Science UNJ. The methods used was experiment with fully randomized design. Factors that tested was 6 gamma irradiation doses (0, 20, 30, 40, 50, 60 Gy) with 10 repetition. Observation of phenotypic generate diverse characters on the growth of the number of shoots and leaves. Gamma irradiation dose of 50 Gy is doses most inhibits the growth of character. Mutations that occur in banana plantlets cv. Kepok generated by the treatment doses gamma irradiation induced mutation is random.
VARIASI STRUKTUR VOKALISASI OWA JAWA (Hylobates moloch Audebert, 1798) DI HUTAN LINDUNG PETUNGKRIYONO, PEKALONGAN, JAWA TENGAH
Authors: Maida Sity , Sukandar Paskal , Istiadi Yossa
Published: August 2017

Javan gibbon is one of an endemic species of Java with kind of unique vocalizations. Supported by a study which showed that every individual has the different anatomy vocal tract that will produce a different sound. Character vocalizations formed from the structure of vocalizations vary between individuals would be the identity of an individual so that there will be effective communication between the giver signal to the receiver. The objective of research is to find out the structure variation of Javan gibbon vocalizations.The research was accomplished in August-September 2015 at the Petungkriyono Protected Forest, Pekalongan, Central Java. The method used is the Focal Continuous sampling. Ratio coefficient of variation within and between female, indicates that the variable vocalizations vary between individuals, and the correct rate of individual identification yielded bydiscriminant function analysis using variable vocalizations was satisfactory (94,4%). Variable vocalization differentiatoris total great call duration (8,99-13,53 s), total great call duration excluding termination phase, duration pre-trill phase, duration introduction note (0,147-0,612 s), frequency range introduction note (408,54-1209,6 Hz), minimal frequency introduction note, maximal frequency introduction note, minimal frequency second trill note, minimal frequency at end of trill note, and minimal frequency range in trill. These vocalizations potentially allow individual recognition of animals.
ANALISIS BAKTERI Escherichia coli PADA MAKANAN SIAP SAJI DI KANTIN RUMAH SAKIT X DAN KANTIN RUMAH SAKIT Y
Authors: Saridewi Inggit , Pambudi Arief , Ningrum Yulia Fitria
Published: August 2017

The increasing human activity makes the preference for fast food increases. However, some people do not pay attention to the hygiene conditions of food processed from food stalls. Food handlers, equipment utilization, food processing, clean water, and the packaging are the critical points of bacterial contamination. Escherichia coli is a bacterium that usually used as the indicator of food hygiene. The objective of this study is to examine the contamination of coliform bacteria, especially E. coli at two hospital cafeteria by using MPN method and questionnaire regarding the implementation of the basic principles of hygiene. Stages of tests performed that are the presumption test, confirmation test, complementary test, gram stain test, biochemical test IMViC and supported by a questionnaire. From the two locations tested, some samples showed positive result in a presumption test and confirmation test but negatively complementary to biochemical test. This indicates that the sample does not contain E. coli bacteria in food, but there is the possibility of Citrobacter. The negative results of the IMVIC test showed that it is possible bacteria found in the presumption test and confirmation test not E. coli and non-pathogenic bacteria. Based on the results of the questionnaire, most of restaurant owner has understood to served food. Food at the hospital X and Y cafetaria are safe to consume because it has a negative E.coli.
Hubungan Antara Coping Dengan Kualitas Perkawinan Pada Ibu Rumah Tangga
Authors: Sutarjo Ayu Annisa , Hasanah Uswatun , Artanti Guspri Devi
Published: August 2017

Ketika suatu perkawinan sering diwarnai pertengkaran, merasa tidak bahagia, atau masalah lainnya sering kali terpikir untuk segera mengakhiri pernikahan tersebut. Berdasarkan data di Pengadilan Agama Kota Bekasi, tahun 2012 jumlah perkara cerai sebanyak 1.716, tahun 2013 perkara cerai sebanyak 1.750 dan tahun 2014 menjadi 1.825 perkara cerai. Faktor jenis perkara cerai terbanyak setiap tahunnya disebabkan ketidak harmonisan 30%, gangguan pihak ketiga 24%, persoalan ekonomi 20%. Kualitas dalam pernikahan bergantung pada kebahagiaan salah satu pasangan dengan hubungan mereka, sensitivitas mereka satu sama lain, validasi mereka terhadap perasaan satu sama lain, serta keterampilan komunikasi dan manajemen konflik mereka. Tujuan penelitian ini untuk menganalisis hubungan antara coping ibu dengan kualitas perkawinan. Penelitian ini dilaksanakan di Kota Bekasi. Metode penelitian yang digunakan adalah survei. Penelitian ini menggunakan metode penelitian kuantatif asosiatif. Populasi pada penelitian ini adalah ibu rumah tangga di Kota Bekasi, jumlah sampel yang digunakan sebanyak 80 orang. Teknik sampling yang digunakan multistage sampling. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara Coping dengan Kualitas Perkawinan dengan koefisien korelasi sebesar 0,351. Hasil uji signifikansi pada koefisiennya sebesar 3,320 dan dapat disimpulkan bahwa koefisien korelasi adalah signifikan. Koefisien determinasi yang diperoleh sebesar 12,3%, menunjukan variabel Kualitas Perkawinan ditentukan oleh Coping. Persamaan regresi yang dihasilkan menunjukkan bahwa setiap peningkatan satu skor coping dapat menyebabkan peningkatan kualitas perkawinan ibu rumah tangga sebesar 0,39 pada konstanta 43,39. Hasil Uji Hipotesis menyatakan bahwa terdapat hubungan yang positif dan signifikan antara Coping dengan Kualitas Perkawinan pada Ibu rumah tangga di Kota Bekasi.
PENGARUH KELEKATAN KELUARGA TERHADAP ADVERSITY QUOTIENT PADA MAHASISWA BIDIK MISI
Authors: Hikmatussyarifah Hikmatussyarifah , Hasanah Uswatun , Tarma Tarma
Published: August 2017

Adversity quotient adalah suatu kecerdasan dalam menghadapi kesulitaan hidup atau masalah. Seberapa tinggi atau rendahnya tingkat adversity quotient yang dimiliki seseorang akan mempengaruhi bagaimana individu tersebut bisa dengan baik menghadapi kesulitaan hidupnya. Sedangkan dalam penelitian ini data diperoleh dari Humas Bidik Misi sebanyak 2660 mahasiswa yang tercatat sebagai penerima beasiswa bidik misi di Fakultas Teknik, Universitas Negeri Jakarta pada periode angkatan tahun 2012-2015. Dari jumlah tersebut sebanyak 275 mahasiwa tercatat beasiswanya dicabut atau dihentikan. Penelitian ini bertujuan untuk mengetahui pengaruh kelekatan keluarga terhadap adversity quotient. Penelitian ini menggunakan metode survey dengan pendekatan korelasi yang dilakukan pada mahasiswa seluruh Program Studi di Fakultas Teknik, Universitas Negeri Jakarta, Rawamangun, Jakarta Timur. Penelitian ini menggunakan Metode Penelitian Kuantitatif. Populasi pada penelitian ini adalah seluruh mahasiswa Program Studi di Fakultas Teknik, Universitas Negeri Jakarta.  Sampel yang digunakan dalam penelitian ini sebanyak 200 orang. Teknik sampling yang digunakan adalah Teknik Purposive Sampling. Hasil dari penelitian ini menunjukan bahwa tidak terdapat pengaruh yang signifikan antara Kelekatan Keluarga terhadap adversity quotient pada mahasiswa Bidik Misi. Kesimpulan dari penelitian ini adalah Ho diterima, yaitu tidak terdapat pengaruh yang signifikan antara kelekatan keluarga terhadap adversity quotient pada mahasiswa bidik misi. Dari hasil penelitian yang tidak berpengaruh tersebut, jenis kelamin menjadi salah satu faktor penyebab tidak adanya pengaruh kelekatan keluarga terhadap adversity quotient. Karena dapat kita ketahui secara umum anak laki-laki tingkat kelekatan dengan keluarganya tidak terjalin begitu baik.
NILAI ANAK PADA MASYARAKAT BETAWI DIWILAYAH SETUBABAKAN DAN HUBUNGANNYA DENGAN KETAHANAN KELUARGA
Authors: Nurpuspitasari Cindy , Mashabi Nurlaila Abdullah , Muhariati Metty
Published: August 2017

Dalam proses kehidupan sebuah keluarga tidak luput dari suatu masalah untuk menghadapi masalah tersebut dibutuhkan ketangguhan (ketahanan) dalam keluarga agar keluarga tetap hidup harmonis dan damai. Menurut Sunarti (2001) ketahanan keluarga ialah kemampuan keluarga  untuk memenuhi kebutuhan keluarga dengan cara dapat mengelola masalah yang dihadapi dalam keluarga. Dalam hal ini keluarga dari masyarakat Betawi sangat memanfaatkan anggota keluarganya dalam mengatasi masalah dalam keluaga. Penelitian ini bertujuan adalah untuk mengetahui dan menganalisi hubungan antara ketahanan keluarga dengan Nilai Anak pada Masyarakat Betawi di wilayah Setu Babakan Kelurahan Srengseng Sawah, Kecamatan Jagakarsa, Kota Jakarta Selatan. Waktu penelitian dimulai pada bulan Desember 2016. Penelitian ini menggunakan metode survey dengan pendekatan kuantitatif  asosiatif. Populasi penelitian ini adalah keluarga dari masyarakat Betawi asli diwilayah Setu Babakan Jakarta Selatan. Teknik pengambilan sampel yang digunakan pada penelitian ini adalah purposive sampling. Jumlah sampel sebanyak 60 responden keluarga masyarakat asli Betawi yang memiliki anak usia 17 thn keatas. Pengujian hipotesis menggunakan analisis person product moment. Hasil perhitungan koefisien korelasi person product moment menghasilkan Thitung sebesar 2.197 dan Ttabel 2.0017 sehingga dapat disimpulkan bahwa Ho di tolak atau terdapat hubungan positif antara ketahanan keluarga dengan nilai anak yang signifikan. Besarnya koefisien determinasi Ketahanan keluarga sebear 6% terhadap nilai anak sedangkan sisanya 84% di pengaruhi oleh faktor lain.