bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensor Design Optimization of Innovative Low-Power, Large Area FD-MAPS for HEP and Applied Science
Authors: Neubüser Coralie , Corradino T. , Dalla Betta G-F. , De Cilladi L. , Pancheri L.
Published: November 2021

Fully depleted monolithic active pixel sensors (FD-MAPSs) represent a state-of-the-art detector technology and profit from a low material budget and cost for high-energy physics experiments and other fields of research like medical imaging and astro-particle physics. Compared to the MAPS currently in use, fully depleted pixel sensors have the advantage of charge collection by drift, which enables a fast and uniform response overall to the pixel matrix. The functionality of these devices has been shown in previous proof-of-concept productions. In this article, we describe the optimization of the test pixel designs that will be implemented in the first engineering run of the demonstrator chip of the ARCADIA project. These optimization procedures include radiation damage models that have been employed in Technology Computer Aided Design simulations to predict the sensors’ behavior in different working environments.
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing
Authors: Ce Fuquan , Mei Jiaqin , He Haiyan , Zhao Yu , Hu Wenhui , Yu Fengqun , Li Qinfei , Ren Xuesong , Si Jun , Song Hongyuan , Qian Wei
Published: November 2021

Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.
Trichoderma longibrachiatum (TG1) Enhances Wheat Seedlings Tolerance to Salt Stress and Resistance to Fusarium pseudograminearum
Authors: Boamah Solomon , Zhang Shuwu , Xu Bingliang , Li Tong , Calderón-Urrea Alejandro
Published: November 2021

Salinity is abiotic stress that inhibits seed germination and suppresses plant growth and root development in a dose-dependent manner.Fusarium pseudograminearum(Fg) is a plant pathogen that causes wheat crown rot. Chemical control methods against Fg are toxic to the environment and resistance has been observed in wheat crops. Therefore, an alternative approach is needed to manage this devastating disease and the effects of salinity. Our research focused on the mycoparasitic mechanisms ofTrichoderma longibrachiatum(TG1) on Fg and the induction of defenses in wheat seedlings under salt and Fg stress at physiological, biochemical and molecular levels. The average inhibition rate of TG1 against Fg was 33.86%, 36.32%, 44.59%, and 46.62%, respectively, in the four NaCl treatments (0, 50, 100, and 150 mM). The mycoparasitic mechanisms of TG1 against Fg were coiling, penetration, and wrapping of Fg hyphae. In response to inoculation of TG1 with Fg, significant upregulation of cell wall degrading enzymes (CWDEs) was observed. The expression of β-1, 6-glucan synthase (PP4), endochitinase precursor (PH-1), and chitinase (chi18-15) increased by 1. 6, 1. 9, and 1.3-fold on day 14 compared with day 3. Wheat seedlings with combined TG1 + Fg treatments under different NaCl stress levels decreased disease index by an average of 51.89%; increased the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity by an average of 38%, 61%, and 24.96%, respectively; and decreased malondialdehyde (MDA) and hydrogen peroxide (H2O2) content by an average of 44.07% and 41.75% respectively, compared with Fg treated seedlings. The combined TG1 + Fg treatment induced the transcription level of plant defense-related genes resulting in an increase in tyrosin-protein kinase (PR2), chitinase class I (CHIA1), and pathogenesis-related protein (PR1-2) by an average of 1.15, 1.35, and 1.37-fold, respectively compared to Fg treatment. However, the expression levels of phenylalanine ammonia-lyase (PAL) increased 3.40-fold under various NaCl stresses. Our results suggest that TG1 enhances wheat seedling growth and controls wheat crown rot disease by strengthening the plant defense system and upregulating the expression of pathogenesis-related genes under both Fg and salt stress.
Mental Simulation to Promote Exercise Intentions and Behaviors
Authors: Zhong Weitan , Zhang Guoli
Published: November 2021

Mental simulation, which employs specific patterns of imagery, can increase the intention to exercise as well as actual engagement in exercise. The present studies explored the effects of mental simulation on the intention to engage in exercise while regulating emotions. The first study confirmed that mental simulation did promote intentions of participants. The second found that video-primed mental simulation was a more effective method of exercise intention promotion than semantic-primed and image-primed mental simulation. In the third study, it was found that combining process-based and outcome-based mental simulations increased exercise intentions. Positive emotions mediated imagery ability and intention to exercise. The final study found that the mental simulation interventions most effective for exercise adherence were those that balanced the valence of process and outcome components in such a way that a challenging process results in a positive outcome, or a smooth process results in a negative outcome. Each of these results has practical implications for exercise interventions that will be discussed.
Cross-Cultural Validation of a New Version in Spanish of Four Items of the Preventive COVID-19 Infection Behaviors Scale (PCIBS) in Twelve Latin American Countries
Authors: Caycho-Rodríguez Tomás , Vilca Lindsey W. , Valencia Pablo D. , Carbajal-León Carlos , Vivanco-Vidal Andrea , Saroli-Araníbar Daniela , Reyes-Bossio Mario , White Michel , Rojas-Jara Claudio , Polanco-Carrasco Roberto , Gallegos Miguel , Cervigni Mauricio , Martino Pablo , Palacios Diego Alejandro , Moreta-Herrera Rodrigo , Samaniego-Pinho Antonio , Lobos-Rivera Marlon Elías , Ferrari Ilka Franco , Flores-Mendoza Carmen , Figares Andrés Buschiazzo , Puerta-Cortés Diana Ximena , Corrales-Reyes Ibraín Enrique , Calderón Raymundo , Tapia Bismarck Pinto , Gallegos Walter L. Arias
Published: November 2021

The invariance of the Preventive COVID-19 Infection Behaviors Scale (PCIBS) was evaluated in 12 Latin American countries (Argentina, Bolivia, Chile, Colombia, Cuba, Ecuador, El Salvador, Guatemala, Mexico, Paraguay, Peru, and Uruguay). A total of 5183 people from the aforementioned countries participated, selected using the snowball sampling method. Measurement invariance was assessed by multigroup confirmatory factor analysis (MG-CFA) and Multi-Group Factor Analysis Alignment (CFA-MIAL). In addition, item characteristics were assessed based on Item Response Theory. The results indicate that the original five-item version of the PCIBS is not adequate; whereas a four-item version of the PCIBS (PCIBS-4) showed a good fit in all countries. Thus, using the MG-CFA method, the PCIBS-4 achieved metric invariance, while the CFA-MIAL method indicated that the PCIBS-4 shows metric and scalar invariance. Likewise, the four items present increasing difficulties and high values in the discrimination parameters. The comparison of means of the PCIBS-4 reported irrelevant differences between countries; however, Mexico and Peru presented the highest frequency of preventive behaviors related to COVID-19. It is concluded that the PCIBS-4 is a unidimensional self-report measure which is reliable and invariant across the twelve participating Latin American countries. It is expected that the findings will be of interest to social and health scientists, as well as those professionals directly involved in public health decision making.
Measuring Staff Attitudes to Coercion in Poland
Authors: Lickiewicz Jakub , Husum Tonje Lossius , Ruud Torleif , Siqveland Johan , Musiał Zofia , Makara-Studzińska Marta
Published: November 2021

Introduction: Coercion can be defined as the use of force to limit a person's choices. In Poland, coercive measures may tend to be overused. However, there is limited information regarding the attitudes of nurses toward coercion in psychiatric settings and the factors influencing any decisions to use coercion.

Aims: To validate the Staff Attitudes to Coercion Scale (SACS) for a group of psychiatric nurses and psychiatrists, to compare the said with the original Norwegian SACS version, and to compare nurses' attitudes with those displayed by psychiatrists. A second aim was to understand the relationship between self-efficacy and attitudes to coercion.

Method: We surveyed 351 psychiatric nurses and psychiatrists rating SACS and GSES (General Self Efficacy Scale). We validated the SACS factor structure using confirmatory principal component factor analysis, calculated the internal consistency of subscales, and analyzed the test-retest reliability and face validity of the subscales themselves. Further, we analyzed the differences in attitudes toward coercion between nurses and psychiatrists, as well as whether there was an association between GSES and the SACS subscales. We compared the means on the SACS items between three countries—Germany, Norway, and Poland.

Results: The confirmatory factor analysis of the Polish version of SACS found the same factor structure with three factors as was displayed in the original Norwegian SACS, except that one item was loaded on another factor. Internal consistency was acceptable for the factors on coercion as security and the coercion as offending, and unacceptable for the factor on coercion as treatment. Test-retest reliability was excellent for all the three subscales. Face validity was high for the factor coercion as security, partly present for coercion as offending, and not present for coercion as treatment. The subscale Coercion as Treatment was rated significantly higher by nurses than by psychiatrists, but there was no difference for the two other subscales. There was no significant association between the General Self-Efficacy Scale and any of the SACS subscales. The biggest differences in attitudes toward forms of coercion was noted between Poland and Germany.

Discussion: The three-factor structure of SACS was the best solution for the Polish nurses and psychiatrists. The attitudes toward coercion differed between the two groups, but a low correlation was computed for the SACS subscales and self-efficacy. There is a cultural diversity visible amongst the three countries examined. Reduction in the use of coercion is a priority worldwide. More knowledge about the process involved in using coercive measures may contribute to this. The use of coercive interventions may harm patients and threaten patients' rights. Thus, education is needed for pre-service and in-service nurses alike.
Obstacles Affect Perceptions of Egocentric Distances in Virtual Environments
Authors: Mine Daisuke , Kimoto Sakurako , Yokosawa Kazuhiko
Published: November 2021

Distance perception in humans can be affected by oculomotor and optical cues and a person’s action capability in a given environment, known as action-specific effects. For example, a previous study has demonstrated that egocentric distance estimation to a target is affected by the width of a transparent barrier placed in the intermediate space between a participant and a target. However, the characteristics of a barrier’s width that affect distance perception remain unknown. Therefore, we investigated whether visual and tactile inputs and actions related to a barrier affect distance estimation to a target behind the barrier. The results confirmed previous studies by demonstrating that visual and tactile presentations of the barrier’s width affected distance estimation to the target. However, this effect of the barrier’s width was not observed when the barrier was touchable but invisible nor when the barrier was visible but penetrable. These findings indicate the complexity of action-specific effects and the difficulty of identifying necessary information for inducing these effects.
Contribution, Utilization, and Improvement of Legumes-Driven Biological Nitrogen Fixation in Agricultural Systems
Authors: Kebede Erana
Published: November 2021

Legumes improve soil fertility through the symbiotic association with microorganisms, such as rhizobia, which fix the atmospheric nitrogen and make nitrogen available to the host and other crops by a process known as biological nitrogen fixation (BNF). Legumes included in the cropping system improve the fertility of the soil and the yield of crops. The advantages of legumes in the cropping system are explained in terms of direct nitrogen transfer, residual fixed nitrogen, nutrient availability and uptake, effect on soil properties, breaking of pests' cycles, and enhancement of other soil microbial activity. The best benefits from the legumes and BNF system can be utilized by integrating them into cropping systems. The most common practices to integrate legumes and their associated BNF into agricultural systems are crop rotation, simultaneous intercropping, improved fallows, green manuring, and alley cropping. However, the level of utilizing nitrogen fixation requires improvement of the systems, such as selecting appropriate legume genotypes, inoculation with effective rhizobia, and the use of appropriate agronomic practices and cropping systems. Therefore, using legumes at their maximum genetic potential, inoculation of legumes with compatible rhizobia, and using appropriate agronomic practices and cropping systems are very important for increasing food production. Importantly, the utilization of legumes as an integral component of agricultural practice in promoting agricultural productivity has gained more traction in meeting the demand of food production of the world populace. Priority should, thus, be given to value the process of BNF through more sustainable technologies and expansion of knowledge to the system.
Intracellular Staphylococcus aureus Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial Cells
Authors: Chen Yuhao , Ma Yuze , Ji Qiang , Yang Xiaoru , Feng Xue , Yao Ruiyuan , Cheng Xiaoou , Li Tingting , Wang Yanfeng , Wang Zhigang
Published: November 2021

Staphylococcus aureus (S. aureus) is one of the main pathogens in cow mastitis, colonizing mammary tissues and being internalized into mammary epithelial cells, causing intracellular infection in the udder. Milk that is produced by cows that suffer from mastitis due to S. aureus is associated with decreased production and changes in protein composition. However, there is limited information on how mastitis-inducing bacteria affect raw milk, particularly with regard to protein content and protein composition. The main purpose of this work was to examine how S. aureus infection affects milk protein synthesis in bovine mammary epithelial cells (BMECs). BMECs were infected with S. aureus, and milk protein and amino acid levels were determined by ELISA after S. aureus invasion. The activity of mTORC1 signaling and the transcription factors NF-κB and STAT5 and the expression of the amino acid transporters SLC1A3 and SLC7A5 were measured by western blot or immunofluorescence and RT-qPCR. S. aureus was internalized by BMECs in vitro, and the internalized bacteria underwent intracellular proliferation. Eight hours after S. aureus invasion, milk proteins were downregulated, and the level of BMECs that absorbed Glu, Asp, and Leu from the culture medium and the exogenous amino acids induced β-casein synthesis declined. Further, the activity of mTORC1 signaling, NF-κB, and STAT5 was impaired, and SLC1A3 and SLC7A5 were downregulated. Eight hours of treatment with 100 nM rapamycin inhibited NF-κB and STAT5 activity, SLC1A3 and SLC7A5 expression, and milk protein synthesis in BMECs. Thus mTORC1 regulates the expression of SLC1A3 and SLC7A5 through NF-κB and STAT5. These findings constitute a model by which S. aureus infection suppresses milk protein synthesis by decreasing amino acids uptake in BMECs.
Is One Teacher Model Enough to Transfer Knowledge to a Student Model?
Authors: Landro Nicola , Gallo Ignazio , La Grassa Riccardo
Published: November 2021

Nowadays, the transfer learning technique can be successfully applied in the deep learning field through techniques that fine-tune the CNN’s starting point so it may learn over a huge dataset such as ImageNet and continue to learn on a fixed dataset to achieve better performance. In this paper, we designed a transfer learning methodology that combines the learned features of different teachers to a student network in an end-to-end model, improving the performance of the student network in classification tasks over different datasets. In addition to this, we tried to answer the following questions which are in any case directly related to the transfer learning problem addressed here. Is it possible to improve the performance of a small neural network by using the knowledge gained from a more powerful neural network? Can a deep neural network outperform the teacher using transfer learning? Experimental results suggest that neural networks can transfer their learning to student networks using our proposed architecture, designed to bring to light a new interesting approach for transfer learning techniques. Finally, we provide details of the code and the experimental settings.
Management of Acute Myeloid Leukemia: Current Treatment Options and Future Perspectives
Authors: Fleischmann Maximilian , Schnetzke Ulf , Hochhaus Andreas , Scholl Sebastian
Published: November 2021

Treatment of acute myeloid leukemia (AML) has improved in recent years and several new therapeutic options have been approved. Most of them include mutation-specific approaches (e.g., gilteritinib for AML patients with activating FLT3 mutations), or are restricted to such defined AML subgroups, such as AML-MRC (AML with myeloid-related changes) or therapy-related AML (CPX-351). With this review, we aim to present a comprehensive overview of current AML therapy according to the evolved spectrum of recently approved treatment strategies. We address several aspects of combined epigenetic therapy with the BCL-2 inhibitor venetoclax and provide insight into mechanisms of resistance towards venetoclax-based regimens, and how primary or secondary resistance might be circumvented. Furthermore, a detailed overview on the current status of AML immunotherapy, describing promising concepts, is provided. This review focuses on clinically important aspects of current and future concepts of AML treatment, but will also present the molecular background of distinct targeted therapies, to understand the development and challenges of clinical trials ongoing in AML patients.
Energy Poverty and Sustainable Economic Development: An Exploration of Correlations and Interdependencies in European Countries
Authors: Tundys Blanka , Bretyn Agnieszka , Urbaniak Maciej
Published: November 2021

The problem of energy poverty exists in practically every European country. Its size and scope are determined by a variety of factors, ranging from economic development to the direction of energy and climate policy implementation to cultural factors. Our aim in this paper was to carry out a comparative analysis of indicators related to energy poverty and sustainable development to identify correlations and links between the two issues and determine how they are related. The fact that the analysis was performed for most European countries is new and represents a broad spectrum of research; we were not limited to studies of countries bound by formal political-economic arrangements or by consideration of the degree of economic development. This approach enabled explication of how diverse the situation is in Europe. The research methods used included a critical analysis of the literature and the use of descriptive and mathematical-statistical tools. The main conclusions and findings of the analysis were that in some countries in economically developed Europe, energy poverty is a major problem, and that, in this respect, there are large differences between “old European Union” and “new European Union” countries, and in the countries that do not belong to political-economic structures in Europe. It is clear, from the research, which countries are rapidly and effectively reducing their energy poverty problems and which factors are the determinants of this. These results are linked to the new direction of energy policy and the shift towards more environmentally friendly energy use. In conclusion, it has been possible to identify the causes of energy poverty and how the energy poverty situation in Europe is changing.
Integrated Capacitive Deionization and Humidification-Dehumidification System for Brackish Water Desalination
Authors: Soomro Sadam-Hussain , Jande Yusufu Abeid Chande , Memon Salman , Kim Woo-Seung , Kim Young-Deuk
Published: November 2021

A hybrid capacitive deionization and humidification-dehumidification (CDI–HDH) desalination system is theoretically investigated for the desalination of brackish water. The CDI system works with two basic operations: adsorption and regeneration. During adsorption, water is desalted, and during the regeneration process the ions from electrodes are detached and flow out as wastewater, which is higher in salt concentration. This wastewater still contains water but cannot be treated again via the CDI unit because CDI cannot treat higher-salinity waters. The discarding of wastewater from CDI is not a good option, since every drop of water is precious. Therefore, CDI wastewater is treated using waste heat in a process that is less sensitive to high salt concentrations, such as humidification-dehumidification (HDH) desalination. Therefore, in this study, CDI wastewater was treated using the HDH system. Using the combined system (CDI–HDH), this study theoretically investigated brackish water of various salt concentrations and flow rates at the CDI inlet. A maximum distillate of 1079 L/day was achieved from the combined system and the highest recovery rate achieved was 24.90% from the HDH unit. Additionally, two renewable energy sources with novel ideas are recommended to power the CDI–HDH system.
Modeling and Sizing of a Fuel Cell—Lithium-Ion Battery Direct Hybridization System for Aeronautical Application
Authors: Jarry Thomas , Lacressonnière Fabien , Jaafar Amine , Turpin Christophe , Scohy Marion
Published: November 2021

Nowadays, many aircraft manufacturers are working on new airplanes to reduce the environmental footprint and therefore meet greenhouse gas reduction targets. The concept of more electric aircraft is one of the solutions to achieve this goal. For this aircraft architecture, several electrical devices are used in order to supply propulsive and non-propulsive functions. This paper focuses on the sizing of a direct hybridization system to supply a non-propulsive function in an aircraft. It is composed of a High-Temperature Proton Exchange Membrane Fuel Cell (HT-PEMFC) and a lithium-ion (Li-ion) battery. This sizing is based on a static model of each storage device. The accuracy of these models is compared with dynamic models during a simulation for an aeronautical mission. Static models are implemented in a genetic algorithm to achieve two goals: on the one hand, satisfy the mission profile, and on the other hand, minimize the mass of the system. Other criteria, such as battery and fuel cell aging estimation, are considered. The obtained results show that the direct hybridization system allows protecting the fuel cell against an accelerated aging.
Reliability and Maintenance Management Analysis on OffShore Wind Turbines (OWTs)
Authors: V. Taboada Jose , Diaz-Casas Vicente , Yu Xi
Published: November 2021

Due to the extreme marine operating environment, the remoteness from the maintenance base, and the expensive specialized accessibility and overhaul equipment needed (e.g., barges, boats, ships, and vessels), offshore O&M costs are greater than those for onshore-based installations. In the operation of wind farms, the main challenges are related to sudden and unexpected failures and downtimes. This paper has three main objectives. The first is to compare and optimize implementation techniques for maintenance strategies. The second is to analyze the cost-benefit of each maintenance strategy model. The third objective is to demonstrate the optimization and effectiveness of maintenance procedures and strategies recreated with stochastic and probabilistic life cycle cost (LCC) models, depending upon the degree of reliability and the maintenance process for offshore wind farms. The cost of operation and maintenance is directly dependent on failure rates, spare parts costs, and the time required by technicians to perform each task in the maintenance program. Calculations for each case study, with either light vessel/transfer boats (Alternative 1) or oilfield support vessels (Alternative 2), focused on the operational costs for transportation. In addition, each case study demonstrated which maintenance conditions and strategies are operational and optimal, and their corresponding cost–risk impacts. Results from this paper suggest that O&M costs are highly correlated with maintenance round frequency (offshore trips) and the operating costs for transportation by light vessel/transfer boat (CTV) and oil-field support vessel (FSV). The paper analyzes cumulative lifecycle costs and finds that for long-term life cycles (25 years), the implement of light vessels (Alternative 1) is more suitable and cost-effective. In contrast, oilfield support vessels (Alternative 2) are more expensive to operate, but they guarantee major capabilities, as well as the advantage of achieving the access levels need to efficiently operate. According to the results obtained by the outcome analysis, it can be concluded that the implementation of light vessels (Alternative 1) shows a lower overall LCC (<million $), which is mainly due to the fact that corrective maintenance and minor repairs are less costly. It should be noted that the cost of major repair operations with light vessels in Alternative 1 is still less than the high costs for minor repairs in Alternative 2 (with FSV).
Novel Dual Walling Cob Building: Dynamic Thermal Performance
Authors: Zeghari Kaoutar , Gounni Ayoub , Louahlia Hasna , Marion Michael , Boutouil Mohamed , Goodhew Steve , Streif François
Published: November 2021

This paper emphasizes the experimental and numerical study of new cob mixes used for insulation and load bearing wall elements. The experimental study provides complete datasets of thermal properties of the new walling materials, using cob with density ranging from 1107 kg/m3 to 1583 kg/m3 for structural walls and less than 700 kg m−3 for insulation walls. Various mixes of French soils and fibres (reed, wheat straw, hemp shiv, hemp straw, and flax straw) with different water contents are studied. The lowest average thermal conductivity is obtained for the structural cob mix prepared of 5% wheat straw and 31% of water content. The insulation mix, prepared with 25% reed and 31% water content, has the lowest thermal conductivity. Investigation of diffusivity, density, and heat capacity shows that, when thermal conductivity is lower than 0.4 W m−1 K−1, the decrease in cob density leads to better insulation values and higher heat capacity. Little variation is noticed regarding the density and heat capacity for cob mixes with thermal conductivity higher than 0.4 W m−1 K−1. Furthermore, the non-uniformity of local thermal conductivity and heat losses through the samples is due mainly to the non-uniform distribution of fibres inside the mixes inducing an increase in heat loss up to 50% for structural walls and 25% for insulation walls. Cob thermal properties are used in a comparative simulation case study of a typical house under French and UK climatic conditions. The energy performance of the conventional building is compared to a dual walled cob building, showing remarkable reduction in energy consumption as the cob walls, whilst maintaining comfortable indoor conditions without additional heating.
Comparative Study on Quality of Fuel Pellets from Switchgrass Treated with Different White-Rot Fungi
Authors: Onu Olughu Onu , Tabil Lope G. , Dumonceaux Tim , Mupondwa Edmund , Cree Duncan
Published: November 2021

Fungal pretreatment of switchgrass using Phanerochaete chrysosporium (PC), Trametes versicolor 52J (TV52J), and the Trametes versicolor mutant strain (m4D) under solid-state fermentation was conducted to improve its pellet quality. For all three fungal strains, the fermentation temperature had a significant effect (p < 0.05) on pellet unit density and tensile strength. The p-values of the quadratic models for all the response variables showed highly significant regression models (p < 0.01) except for dimensional stability. In addition, 3.1-fold and 2.8-fold increase in pellet tensile strength were obtained from P. chrysosporium- and T. versicolor 52J-treated materials, respectively. Microstructural examination showed that fungal pretreatment reduced pores in the pellets and enhanced pellet particle bonding. Among the fungal strains, PC had the shortest optimum fermentation time (21 d) and most positive impact on the pellet tensile strength and hydrophobicity. Therefore, switchgrass pretreatment using PC has the potential for resolving the challenges of switchgrass pellet transportation and storage and reducing the overall pelletization cost. However, a detailed comparative technoeconomic analysis would be required to make definitive cost comparisons.
Key Proteins and Metabolic Pathways Involved in 24-Epibrasionlide Improving Drought Tolerance of Rhododendron delavayi Franch
Authors: Cai Yan-Fei , Zhang Lu , Peng Lv-Chun , Li Shi-Feng , Song Jie , Xie Wei-Jia , Wang Ji-Hua
Published: November 2021

Rhododendron delavayi Franch. is a famous ornamental plant. However, seasonal drought caused by a monsoon climate seriously limits its growth and flowering performance in urban gardens. Our previous study has found that brassinosteroids (BRs) can improve the tolerance of R. delavayi to drought stress. Here, we employed a data-independent acquisition (DIA) approach to compare the protein expression profiles under drought treatment (D) and pre-treatment with BR before drought treatment (BR). With an increase in drought stress, the net photosynthetic rate, stomatal conductance, and transpiration rate in the BR treatment showed more stable changes that were significantly higher than those in the D treatment. However, the contents of malondialdehyde, soluble sugar, soluble protein, and the activity of superoxide dismutase (SOD), peroxidase, and catalase showed opposite trends. The pre-treatment with BR alleviated the negative effect of drought stress on the photosynthetic performance of R. delavayi. A total of 3453 differentially expressed proteins (DEPs) were identified, and 683 DEPs were significantly expressed in the D and BR treatments. The DEPs uniquely expressed in the BR treatment participated in the pathways of “ribosome”, “ether lipid metabolism”, “photosynthesis”, and “oxidative phosphorylation”. The improvement effect of the BR treatment on the drought tolerance of R. delavayi was mainly attributed to improved photosynthesis by alleviating stomatal closure and oxidative stress, maintaining the integrity and stability of the ribosomal complex to mediate protein synthesis and the balance between energy metabolism and carbon metabolism. Our study presents a comprehensive understanding of the key proteins and metabolic pathways related to the response of R. delavayi to drought and will contribute to the breeding of drought-tolerant rhododendrons.
Exercise Training Alleviates Cardiac Fibrosis through Increasing Fibroblast Growth Factor 21 and Regulating TGF-β1-Smad2/3-MMP2/9 Signaling in Mice with Myocardial Infarction
Authors: Ma Yixuan , Kuang Yixin , Bo Wenyan , Liang Qiaoqin , Zhu Wenfei , Cai Mengxin , Tian Zhenjun
Published: November 2021

Exercise training has been reported to alleviate cardiac fibrosis and ameliorate heart dysfunction after myocardial infarction (MI), but the molecular mechanism is still not fully clarified. Fibroblast growth factor 21 (FGF21) exerts a protective effect on the infarcted heart. This study investigates whether exercise training could increase FGF21 protein expression and regulate the transforming growth factor-β1 (TGF-β1)-Smad2/3-MMP2/9 signaling pathway to alleviate cardiac fibrosis following MI. Male wild type (WT) C57BL/6J mice and Fgf21 knockout (Fgf21 KO) mice were used to establish the MI model and subjected to five weeks of different types of exercise training. Both aerobic exercise training (AET) and resistance exercise training (RET) significantly alleviated cardiac dysfunction and fibrosis, up-regulated FGF21 protein expression, inhibited the activation of TGF-β1-Smad2/3-MMP2/9 signaling pathway and collagen production, and meanwhile, enhanced antioxidant capacity and reduced cell apoptosis in the infarcted heart. In contrast, knockout of Fgf21 weakened the cardioprotective effects of AET after MI. In vitro, cardiac fibroblasts (CFs) were isolated from neonatal mice hearts and treated with H2O2 (100 μM, 6 h). Recombinant human FGF21 (rhFGF21, 100 ng/mL, 15 h) and/or 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR, 1 mM, 15 h) inhibited H2O2-induced activation of the TGF-β1-Smad2/3-MMP2/9 signaling pathway, promoted CFs apoptosis and reduced collagen production. In conclusion, exercise training increases FGF21 protein expression, inactivates the TGF-β1-Smad2/3-MMP2/9 signaling pathway, alleviates cardiac fibrosis, oxidative stress, and cell apoptosis, and finally improves cardiac function in mice with MI. FGF21 plays an important role in the anti-fibrosis effect of exercise training.
A Comparison Study on the Magneto-Responsive Properties and Swelling Behaviors of a Polyacrylamide-Based Hydrogel Incorporating with Magnetic Particles
Authors: Xu Chanchan , Li Bin , Wang Xiaojie
Published: November 2021

This work investigates the mechanical properties, microstructures, and water-swelling behavior of a novel hydrogel filled with magnetic particles. The nanoparticles of magnetite (Fe3O4) and the micro-particles of carbonyl iron (CI) were selected and filled into a polyacrylamide (PAAM) hydrogel matrix to create two types of magnetic hydrogels. The isotropy and anisotropy of magnetic hydrogels are also presented in this study. The isotropic samples were cured without applying a magnetic field (MF), and the anisotropic samples were cured by applying an MF in the direction perpendicular to the thickness of the samples. The effects of the size, content, and inner structures of magnetic particles on the magneto-responsive and swelling properties of magnetic hydrogels were investigated. It was found that the magnetorheological (MR) effect of anisotropic samples was apparently higher than that of isotropic samples, and the hydrogels with CI exhibited a noticeable MR effect than those with Fe3O4. The storage modulus can be enhanced by increasing the filler content and size, forming an anisotropic structure, and applying an external MF. In addition, the magnetic hydrogels also have a swelling ability that can be tuned by varying the content and size of the particle fillers.
Uniaxial Hydroxyapatite Growth on a Self-Assembled Protein Scaffold
Authors: Danesi Alexander L. , Athanasiadou Dimitra , Mansouri Ahmad , Phen Alina , Neshatian Mehrnoosh , Holcroft James , Bonde Johan , Ganss Bernhard , Carneiro Karina M. M.
Published: November 2021

Biomineralization is a crucial process whereby organisms produce mineralized tissues such as teeth for mastication, bones for support, and shells for protection. Mineralized tissues are composed of hierarchically organized hydroxyapatite crystals, with a limited capacity to regenerate when demineralized or damaged past a critical size. Thus, the development of protein-based materials that act as artificial scaffolds to guide hydroxyapatite growth is an attractive goal both for the design of ordered nanomaterials and for tissue regeneration. In particular, amelogenin, which is the main protein that scaffolds the hierarchical organization of hydroxyapatite crystals in enamel, amelogenin recombinamers, and amelogenin-derived peptide scaffolds have all been investigated for in vitro mineral growth. Here, we describe uniaxial hydroxyapatite growth on a nanoengineered amelogenin scaffold in combination with amelotin, a mineral promoting protein present during enamel formation. This bio-inspired approach for hydroxyapatite growth may inform the molecular mechanism of hydroxyapatite formation in vitro as well as possible mechanisms at play during mineralized tissue formation.
Comparative Analysis of Coding and Non-Coding Features within Insect Tolerance Loci in Wheat with Their Homologs in Cereal Genomes
Authors: Muslu Tugdem , Akpinar Bala Ani , Biyiklioglu-Kaya Sezgi , Yuce Meral , Budak Hikmet
Published: November 2021

Food insecurity and malnutrition have reached critical levels with increased human population, climate fluctuations, water shortage; therefore, higher-yielding crops are in the spotlight of numerous studies. Abiotic factors affect the yield of staple food crops; among all, wheat stem sawfly (Cephus cinctus Norton) and orange wheat blossom midge (Sitodiplosis mosellana) are two of the most economically and agronomically harmful insect pests which cause yield loss in cereals, especially in wheat in North America. There is no effective strategy for suppressing this pest damage yet, and only the plants with intrinsic tolerance mechanisms such as solid stem phenotypes for WSS and antixenosis and/or antibiosis mechanisms for OWBM can limit damage. A major QTL and a causal gene for WSS resistance were previously identified in wheat, and 3 major QTLs and a causal gene for OWBM resistance. Here, we present a comparative analysis of coding and non-coding features of these loci of wheat across important cereal crops, barley, rye, oat, and rice. This research paves the way for our cloning and editing of additional WSS and OWBM tolerance gene(s), proteins, and metabolites.
Special Issue “Microglia Heterogeneity and Its Relevance for Translational Research”
Authors: Michelucci Alessandro , Miron Veronique E. , Priller Josef
Published: November 2021

Microglia are necessary for the development and function of the central nervous system (CNS) [...]
A Hypothetical Model Suggesting Some Possible Ways That the Progesterone Receptor May Be Involved in Cancer Proliferation
Authors: Check Jerome H. , Check Diane L.
Published: November 2021

Cancer and the fetal-placental semi-allograft share certain characteristics, e.g., rapid proliferation, the capacity to invade normal tissue, and, related to the presence of antigens foreign to the host, the need to evade immune surveillance. Many present-day methods to treat cancer use drugs that can block a key molecule that is important for one or more of these characteristics and thus reduce side effects. The ideal molecule would be one that is essential for both the survival of the fetus and malignant tumor, but not needed for normal cells. There is a potential suitable candidate, the progesterone induced blocking factor (PIBF). The parent 90 kilodalton (kDa) form seems to be required for cell-cycle regulation, required by both the fetal-placental unit and malignant tumors. The parent form may be converted to splice variants that help both the fetus and tumors escape immune surveillance, especially in the fetal and tumor microenvironment. Evidence suggests that membrane progesterone receptors are involved in PIBF production, and indeed there has been anecdotal evidence that progesterone receptor antagonists, e.g., mifepristone, can significantly improve longevity and quality of life, with few side effects.
The Somatic Mutation Paradigm in Congenital Malformations: Hirschsprung Disease as a Model
Authors: MacKenzie Katherine C. , Garritsen Rhiana , Chauhan Rajendra K. , Sribudiani Yunia , de Graaf Bianca M. , Rugenbrink Tim , Brouwer Rutger , van Ijcken Wilfred F. J. , de Blaauw Ivo , Brooks Alice S. , Sloots Cornelius E. J. , Meeuwsen Conny J. H. M. , Wijnen René M. , Newgreen Donald F. , Burns Alan J. , Hofstra Robert M. W. , Alves Maria M. , Brosens Erwin
Published: November 2021

Patients with Hirschsprung disease (HSCR) do not always receive a genetic diagnosis after routine screening in clinical practice. One of the reasons for this could be that the causal mutation is not present in the cell types that are usually tested—whole blood, dermal fibroblasts or saliva—but is only in the affected tissue. Such mutations are called somatic, and can occur in a given cell at any stage of development after conception. They will then be present in all subsequent daughter cells. Here, we investigated the presence of somatic mutations in HSCR patients. For this, whole-exome sequencing and copy number analysis were performed in DNA isolated from purified enteric neural crest cells (ENCCs) and blood or fibroblasts of the same patient. Variants identified were subsequently validated by Sanger sequencing. Several somatic variants were identified in all patients, but causative mutations for HSCR were not specifically identified in the ENCCs of these patients. Larger copy number variants were also not found to be specific to ENCCs. Therefore, we believe that somatic mutations are unlikely to be identified, if causative for HSCR. Here, we postulate various modes of development following the occurrence of a somatic mutation, to describe the challenges in detecting such mutations, and hypothesize how somatic mutations may contribute to ‘missing heritability’ in developmental defects.
Enigmatic Pilus-Like Endospore Appendages of Bacillus cereus Group Species
Authors: Zegeye Ephrem Debebe , Pradhan Brajabandhu , Llarena Ann-Katrin , Aspholm Marina
Published: November 2021

The endospores (spores) of many Bacillus cereus sensu lato species are decorated with multiple hair/pilus-like appendages. Although they have been observed for more than 50 years, all efforts to characterize these fibers in detail have failed until now, largely due to their extraordinary resilience to proteolytic digestion and chemical solubilization. A recent structural analysis of B. cereus endospore appendages (Enas) using cryo-electron microscopy has revealed the structure of two distinct fiber morphologies: the longer and more abundant “Staggered-type” (S-Ena) and the shorter “Ladder-like” type (L-Ena), which further enabled the identification of the genes encoding the S-Ena. Ena homologs are widely and uniquely distributed among B. cereus sensu lato species, suggesting that appendages play important functional roles in these species. The discovery of ena genes is expected to facilitate functional studies involving Ena-depleted mutant spores to explore the role of Enas in the interaction between spores and their environment. Given the importance of B. cereus spores for the food industry and in medicine, there is a need for a better understanding of their biological functions and physicochemical properties. In this review, we discuss the current understanding of the Ena structure and the potential roles these remarkable fibers may play in the adhesion of spores to biotic and abiotic surfaces, aggregation, and biofilm formation.
Modeling, Synthesis, and Biological Evaluation of Potential Retinoid-X-Receptor (RXR) Selective Agonists: Analogs of 4-[1-(3,5,5,8,8-Pentamethyl-5,6,7,8-tetrahyro-2-naphthyl)ethynyl]benzoic Acid (Bexarotene) and 6-(Ethyl(4-isobutoxy-3-isopropylphenyl)amino)nicotinic Acid (NEt-4IB)
Authors: Jurutka Peter W. , di Martino Orsola , Reshi Sabeeha , Mallick Sanchita , Sabir Zhela L. , Staniszewski Lech J. P. , Warda Ankedo , Maiorella Emma L. , Minasian Ani , Davidson Jesse , Ibrahim Samir J. , Raban San , Haddad Dena , Khamisi Madleen , Suban Stephanie L. , Dawson Bradley J. , Candia Riley , Ziller Joseph W. , Lee Ming-Yue , Liu Chang , Liu Wei , Marshall Pamela A. , Welch John S. , Wagner Carl E.
Published: November 2021

Five novel analogs of 6-(ethyl)(4-isobutoxy-3-isopropylphenyl)amino)nicotinic acid—or NEt-4IB—in addition to seven novel analogs of 4-[1-(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydro-2-naphthyl)ethynyl]benzoic acid (bexarotene) were prepared and evaluated for selective retinoid-X-receptor (RXR) agonism alongside bexarotene (1), a FDA-approved drug for cutaneous T-cell lymphoma (CTCL). Bexarotene treatment elicits side-effects by provoking or disrupting other RXR-dependent pathways. Analogs were assessed by the modeling of binding to RXR and then evaluated in a human cell-based RXR-RXR mammalian-2-hybrid (M2H) system as well as a RXRE-controlled transcriptional system. The analogs were also tested in KMT2A-MLLT3 leukemia cells and the EC50 and IC50 values were determined for these compounds. Moreover, the analogs were assessed for activation of LXR in an LXRE system as drivers of ApoE expression and subsequent use as potential therapeutics in neurodegenerative disorders, and the results revealed that these compounds exerted a range of differential LXR-RXR activation and selectivity. Furthermore, several of the novel analogs in this study exhibited reduced RARE cross-signaling, implying RXR selectivity. These results demonstrate that modification of partial agonists such as NEt-4IB and potent rexinoids such as bexarotene can lead to compounds with improved RXR selectivity, decreased cross-signaling of other RXR-dependent nuclear receptors, increased LXRE-heterodimer selectivity, and enhanced anti-proliferative potential in leukemia cell lines compared to therapeutics such as 1.