bluuebunny/crossref_metadata_2025 huggingface dataset:
Learning Feedforward Control Using Multiagent Control Approach for Motion Control Systems
Authors: Dao Phong B.
Published: January 2021

Multiagent control system (MACS) has become a promising solution for solving complex control problems. Using the advantages of MACS-based design approaches, a novel solution for advanced control of mechatronic systems has been developed in this paper. The study has aimed at integrating learning control into MACS. Specifically, learning feedforward control (LFFC) is implemented as a pattern for incorporation in MACS. The major novelty of this work is that the feedback control part is realized in a real-time periodic MACS, while the LFFC algorithm is done on-line, asynchronously, and in a separate non-real-time aperiodic MACS. As a result, a MACS-based LFFC design method has been developed. A second-order B-spline neural network (BSN) is used as a function approximator for LFFC whose input-output mapping can be adapted during control and is intended to become equal to the inverse model of the plant. To provide real-time features for the MACS-based LFFC system, the open robot control software (OROCOS) has been employed as development and runtime environment. A case study using a simulated linear motor in the presence of nonlinear cogging and friction force as well as mass variations is used to illustrate the proposed method. A MACS-based LFFC system has been designed and implemented for the simulated plant. The system consists of a setpoint generator, a feedback controller, and a time-index LFFC that can learn on-line. Simulation results have demonstrated the applicability of the design method.
Epigenetic Regulators of White Adipocyte Browning
Authors: Nanduri Ravikanth
Published: January 2021

Adipocytes play an essential role in maintaining energy homeostasis in mammals. The primary function of white adipose tissue (WAT) is to store energy; for brown adipose tissue (BAT), primary function is to release fats in the form of heat. Dysfunctional or excess WAT can induce metabolic disorders such as dyslipidemia, obesity, and diabetes. Preadipocytes or adipocytes from WAT possess sufficient plasticity as they can transdifferentiate into brown-like beige adipocytes. Studies in both humans and rodents showed that brown and beige adipocytes could improve metabolic health and protect from metabolic disorders. Brown fat requires activation via exposure to cold or β-adrenergic receptor (β-AR) agonists to protect from hypothermia. Considering the fact that the usage of β-AR agonists is still in question with their associated side effects, selective induction of WAT browning is therapeutically important instead of activating of BAT. Hence, a better understanding of the molecular mechanisms governing white adipocyte browning is vital. At the same time, it is also essential to understand the factors that define white adipocyte identity and inhibit white adipocyte browning. This literature review is a comprehensive and focused update on the epigenetic regulators crucial for differentiation and browning of white adipocytes.
Plasticity of Leaf Traits of Juglans regia L. f. luodianense Liu et Xu Seedlings Under Different Light Conditions in Karst Habitats
Authors: Wang Deng , Huang XiaoLong , Chen JingZhong , Li LiXia , Cheng Jing , Wang Shu , Liu JiMing
Published: January 2021

This study examined the effects of light intensity on the plasticity of the leaves of Juglans regia f. luodianense seedlings in karst habitat and how they respond to changes in light intensity. The light intensity of 1-year-old seedlings of J. regia f. luodianense in different niches in a karst area was set as 100% (bare land), 75% (forest margin), 50% (forest gap), and 25% (under forest) of natural light. The material harvested after four months was compared to analyze the differences in various morphological characteristics, biomass allocation, and physiological characteristics of the leaves of seedlings of J. regia f. luodianense, and a comprehensive evaluation of the plasticity indexes was conducted. The results showed that under moderate (50%) full light intensity, the leaf area, specific leaf area, leaf biomass, and chlorophyll content increased, and improved photosynthesis and promoted the accumulation of free proline content and peroxidase (POD) activity. The accumulation of malondialdehyde was also the lowest in this treatment, indicating that the plants had the strongest adaptability under this light intensity. Moreover, under high (75%) full light intensity, the above functional characteristics of plants showed good performance. Under low (25%) full light intensity, plants also had higher specific leaf area, leaf biomass, and photosynthetic parameters. However, under full light, the cell membrane permeability decreased, the chlorophyll accumulation was the lowest, and the photosynthetic index was seriously inhibited. Our results showed that the plasticity of morphological characters was greater than that of biomass allocation and physiological characters; POD activity and stomatal conductance were the highest, followed by leaf area and chlorophyll b, whereas the plasticity of palisade tissue/sponge tissue thickness and lower-epidermis thickness were the lowest. In summary, there are evident differences in the sensitivity and regulation mechanisms of morphological characteristics, biomass allocation, and physiological indices of the seedling leaves of J. regia f. luodianense in response to light intensity. During the stage of seedling establishment, only the plants in the bare ground under full light can be induced to show obvious inhibition of phenotypic traits. In contrast, the plants in the forest margins and gaps and under the forest habitats under light intensity can regulate their own characteristics to maintain their growth and development. The wide light range and strong plasticity of the species might be two of the important reasons for its existence in a highly heterogeneous karst habitat.
Soy Protein Pressed Gels: Gelation Mechanism Affects the In Vitro Proteolysis and Bioaccessibility of Added Phenolic Acids
Authors: Marinea Marina , Ellis Ashling , Golding Matt , Loveday Simon M.
Published: January 2021

In this study, a model system of firm tofu (pressed gel) was prepared to study how the coagulation mechanism—acidification with glucono δ-lactone (GDL) or coagulation with magnesium sulphate (MgSO4)—affected the physical properties of the gels along with their in vitro proteolysis (or extent of proteolysis). The two types of gels were also fortified with 3.5 mM protocatechuic (PCA) and coumaric acid (CMA) to test whether they can be used as bioactive delivery systems. Texture analysis showed that all MgSO4-induced gels (fortified and control) had a higher hydration capacity and a weaker texture than the GDL-induced gels (p < 0.05). MgSO4 gels had almost double proteolysis percentages throughout the in vitro digestion and showed a significantly higher amino acid bioaccessibility than the GDL gels (essential amino acid bioaccessibility of 56% versus 31%; p < 0.05). Lastly, both gel matrices showed a similar phenolic acid release profile, on a percentage basis (~80% for PCA and ~100% for CMA). However, GDL gels delivered significantly higher masses of bioactives under simulated intestinal conditions because they could retain more of the bioactives in the gel after pressing. It was concluded that the coagulation mechanism affects both the macro- and microstructure of the soy protein pressed gels and as a result their protein digestibility. Both pressed gel matrices are promising delivery systems for bioactive phenolic acids.
Elemental Analysis and Phenolic Profiles of Selected Italian Wines
Authors: Fermo Paola , Comite Valeria , Sredojević Milica , Ćirić Ivanka , Gašić Uroš , Mutić Jelena , Baošić Rada , Tešić Živoslav
Published: January 2021

The study of the chemical composition of wines is nowadays a topic of great interest because of the importance of this market, especially in Italy, and also considering the numerous cases of falsification of famous and very expensive wines. The present paper focused on the analysis of metals and polyphenols in Italian wines belonging to different provenance and types. At this purpose 20 elements were quantified by inductively coupled plasma optical emission spectrometry (ICP-OES) and ICP mass spectrometry (ICP-MS). Regarding polyphenols, a total of 32 were quantified, among 6 were anthocyanins. Furthermore, in 4 samples (1 rosè and 3 red wines) 42 anthocyanins and related compounds were identified by ultra-high performance liquid chromatography (UHPLC)-Orbitrap MS technique (among these, 6 were also quantified). Non-anthocyanins were determined using UHPLC coupled with a diode array detector and triple-quadrupole mass spectrometer (UHPLC–DAD-QqQ-MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were measured using spectrophotometric methods. The results obtained by elemental techniques were submitted to principal components analysis (PCA) allowing to get information on both geographical and botanical origin of the examined wine samples. Some polyphenols have been detected in higher concentrations only in a certain type of wine, as for example in the case of Grechetto wine. Most of the identified anthocyanin derivatives (pyranoanthocyanins) are formed during the aging of wine by reaction with the other wine components.
Rapid Evaluation and Validation Method of Above Ground Forest Biomass Estimation Using Optical Remote Sensing in Tundi Reserved Forest Area, India
Authors: Kumar Praveen , Krishna Akhouri P. , Rasmussen Thorkild M. , Pal Mahendra K.
Published: January 2021

Optical remote sensing data are freely available on a global scale. However, the satellite image processing and analysis for quick, accurate, and precise forest above ground biomass (AGB) evaluation are still challenging and difficult. This paper is aimed to develop a novel method for precise, accurate, and quick evaluation of the forest AGB from optical remote sensing data. Typically, the ground forest AGB was calculated using an empirical model from ground data for biophysical parameters such as tree density, height, and diameter at breast height (DBH) collected from the field at different elevation strata. The ground fraction of vegetation cover (FVC) in each ground sample location was calculated. Then, the fraction of vegetation cover (FVC) from optical remote sensing imagery was calculated. In the first stage of method implementation, the relation model between the ground FVC and ground forest AGB was developed. In the second stage, the relational model was established between image FVC and ground FVC. Finally, both models were fused to derive the relational model between image FVC and forest AGB. The validation of the developed method was demonstrated utilizing Sentinel-2 imagery as test data and the Tundi reserved forest area located in the Dhanbad district of Jharkhand state in eastern India was used as the test site. The result from the developed model was ground validated and also compared with the result from a previously developed crown projected area (CPA)-based forest AGB estimation approach. The results from the developed approach demonstrated superior capabilities in precision compared to the CPA-based method. The average forest AGB estimation of the test site obtained by this approach revealed 463 tons per hectare, which matches the previous estimate from this test site.
Semantics-Driven Remote Sensing Scene Understanding Framework for Grounded Spatio-Contextual Scene Descriptions
Authors: Potnis Abhishek V. , Durbha Surya S. , Shinde Rajat C.
Published: January 2021

Earth Observation data possess tremendous potential in understanding the dynamics of our planet. We propose the Semantics-driven Remote Sensing Scene Understanding (Sem-RSSU) framework for rendering comprehensive grounded spatio-contextual scene descriptions for enhanced situational awareness. To minimize the semantic gap for remote-sensing-scene understanding, the framework puts forward the transformation of scenes by using semantic-web technologies to Remote Sensing Scene Knowledge Graphs (RSS-KGs). The knowledge-graph representation of scenes has been formalized through the development of a Remote Sensing Scene Ontology (RSSO)—a core ontology for an inclusive remote-sensing-scene data product. The RSS-KGs are enriched both spatially and contextually, using a deductive reasoner, by mining for implicit spatio-contextual relationships between land-cover classes in the scenes. The Sem-RSSU, at its core, constitutes novel Ontology-driven Spatio-Contextual Triple Aggregation and realization algorithms to transform KGs to render grounded natural language scene descriptions. Considering the significance of scene understanding for informed decision-making from remote sensing scenes during a flood, we selected it as a test scenario, to demonstrate the utility of this framework. In that regard, a contextual domain knowledge encompassing Flood Scene Ontology (FSO) has been developed. Extensive experimental evaluations show promising results, further validating the efficacy of this framework.
Multiscale Regulation of the Intervertebral Disc: Achievements in Experimental, In Silico, and Regenerative Research
Authors: Baumgartner Laura , Wuertz-Kozak Karin , Le Maitre Christine L. , Wignall Francis , Richardson Stephen M. , Hoyland Judith , Ruiz Wills Carlos , González Ballester Miguel A. , Neidlin Michael , Alexopoulos Leonidas G. , Noailly Jérôme
Published: January 2021

Intervertebral disc (IVD) degeneration is a major risk factor of low back pain. It is defined by a progressive loss of the IVD structure and functionality, leading to severe impairments with restricted treatment options due to the highly demanding mechanical exposure of the IVD. Degenerative changes in the IVD usually increase with age but at an accelerated rate in some individuals. To understand the initiation and progression of this disease, it is crucial to identify key top-down and bottom-up regulations’ processes, across the cell, tissue, and organ levels, in health and disease. Owing to unremitting investigation of experimental research, the comprehension of detailed cell signaling pathways and their effect on matrix turnover significantly rose. Likewise, in silico research substantially contributed to a holistic understanding of spatiotemporal effects and complex, multifactorial interactions within the IVD. Together with important achievements in the research of biomaterials, manifold promising approaches for regenerative treatment options were presented over the last years. This review provides an integrative analysis of the current knowledge about (1) the multiscale function and regulation of the IVD in health and disease, (2) the possible regenerative strategies, and (3) the in silico models that shall eventually support the development of advanced therapies.
Pluripotent Stem Cells for Disease Modeling and Drug Discovery in Niemann-Pick Type C1
Authors: Völkner Christin , Liedtke Maik , Hermann Andreas , Frech Moritz J.
Published: January 2021

The lysosomal storage disorders Niemann-Pick disease Type C1 (NPC1) and Type C2 (NPC2) are rare diseases caused by mutations in the NPC1 or NPC2 gene. Both NPC1 and NPC2 are proteins responsible for the exit of cholesterol from late endosomes and lysosomes (LE/LY). Consequently, mutations in one of the two proteins lead to the accumulation of unesterified cholesterol and glycosphingolipids in LE/LY, displaying a disease hallmark. A total of 95% of cases are due to a deficiency of NPC1 and only 5% are caused by NPC2 deficiency. Clinical manifestations include neurological symptoms and systemic symptoms, such as hepatosplenomegaly and pulmonary manifestations, the latter being particularly pronounced in NPC2 patients. NPC1 and NPC2 are rare diseases with the described neurovisceral clinical picture, but studies with human primary patient-derived neurons and hepatocytes are hardly feasible. Obviously, induced pluripotent stem cells (iPSCs) and their derivatives are an excellent alternative for indispensable studies with these affected cell types to study the multisystemic disease NPC1. Here, we present a review focusing on studies that have used iPSCs for disease modeling and drug discovery in NPC1 and draw a comparison to commonly used NPC1 models.
Purinergic Receptor Blockade with Suramin Increases Survival of Postnatal Neural Progenitor Cells In Vitro
Authors: Herrera Alejandro , Morcuende Sara , Talaverón Rocío , Benítez-Temiño Beatriz , Pastor Angel M. , Matarredona Esperanza R.
Published: January 2021

Neural progenitor cells (NPCs) are self-renewing and multipotent cells that persist in the postnatal and adult brain in the subventricular zone and the hippocampus. NPCs can be expanded in vitro to be used in cell therapy. However, expansion is limited, since the survival and proliferation of adult NPCs decrease with serial passages. Many signaling pathways control NPC survival and renewal. Among these, purinergic receptor activation exerts differential effects on the biology of adult NPCs depending on the cellular context. In this study, we sought to analyze the effect of a general blockade of purinergic receptors with suramin on the proliferation and survival of NPCs isolated from the subventricular zone of postnatal rats, which are cultured as neurospheres. Treatment of neurospheres with suramin induced a significant increase in neurosphere diameter and in NPC number attributed to a decrease in apoptosis. Proliferation and multipotency were not affected. Suramin also induced an increase in the gap junction protein connexin43 and in vascular endothelial growth factor, which might be involved in the anti-apoptotic effect. Our results offer a valuable tool for increasing NPC survival before implantation in the lesioned brain and open the possibility of using this drug as adjunctive therapy to NPC transplantation.
Topical Application of Mesenchymal Stem Cell Exosomes Alleviates the Imiquimod Induced Psoriasis-Like Inflammation
Authors: Zhang Bin , Lai Ruenn Chai , Sim Wei Kian , Choo Andre Boon Hwa , Lane Ellen Birgit , Lim Sai Kiang
Published: January 2021

Severe psoriasis, a chronic inflammatory skin disease is increasingly being effectively managed by targeted immunotherapy but long-term immunotherapy poses health risk and loss of response. Therefore, there is a need for alternative therapy strategies. Mesenchymal stem/stromal cell (MSC) exosomes are widely known for their potent immunomodulatory properties. Here we investigated if topically applied MSC exosomes could alleviate psoriasis-associated inflammation. Topically applied fluorescent exosomes on human skin explants were confined primarily to the stratum corneum with <1% input fluorescence exiting the explant over a 24-h period. Nevertheless, topically applied MSC exosomes in a mouse model of imiquimod (IMQ) psoriasis significantly reduced IL-17 and terminal complement activation complex C5b-9 in the mouse skin. MSC exosomes were previously shown to inhibit complement activation, specifically C5b-9 complex formation through CD59. Infiltration of neutrophils into the stratum corneum is characteristic of psoriasis and neutrophils are a major cellular source of IL-17 in psoriasis through the release of neutrophil extracellular traps (NETs). We propose that topically applied MSC exosomes inhibit complement activation in the stratum corneum and this alleviates IL-17 release by NETS from neutrophils that accumulate in and beneath the stratum corneum.
Evaluation of the Multimycotoxin-Degrading Efficiency of Rhodococcus erythropolis NI1 Strain with the Three-Step Zebrafish Microinjection Method
Authors: Garai Edina , Risa Anita , Varga Emese , Cserháti Mátyás , Kriszt Balázs , Urbányi Béla , Csenki Zsolt
Published: January 2021

The multimycotoxin-degrading efficiency of the Rhodococcus erythropolis NI1 strain was investigated with a previously developed three-step method. NI1 bacterial metabolites, single and combined mycotoxins and their NI1 degradation products, were injected into one cell stage zebrafish embryos in the same doses. Toxic and interaction effects were supplemented with UHPLC-MS/MS measurement of toxin concentrations. Results showed that the NI1 strain was able to degrade mycotoxins and their mixtures in different proportions, where a higher ratio of mycotoxins were reduced in combination than single ones. The NI1 strain reduced the toxic effects of mycotoxins and mixtures, except for the AFB1+T-2 mixture. Degradation products of the AFB1+T-2 mixture by the NI1 strain were more toxic than the initial AFB1+T-2 mixture, while the analytical results showed very high degradation, which means that the NI1 strain degraded this mixture to toxic degradation products. The NI1 strain was able to detoxify the AFB1, ZEN, T-2 toxins and mixtures (except for AFB1+T-2 mixture) during the degradation experiments, which means that the NI1 strain degraded these to non-toxic degradation products. The results demonstrate that single exposures of mycotoxins were very toxic. The combined exposure of mycotoxins had synergistic effects, except for ZEN+T-2 and AFB1+ZEN +T-2, whose mixtures had very strong antagonistic effects.
Molecular and Genetic Aspects of Grain Number Determination in Rice (Oryza sativa L.)
Authors: Yin Changxi , Zhu Yanchun , Li Xuefei , Lin Yongjun
Published: January 2021

Rice grain yield is a complex trait determined by three components: panicle number, grain number per panicle (GNPP) and grain weight. GNPP is the major contributor to grain yield and is crucial for its improvement. GNPP is determined by a series of physiological and biochemical steps, including inflorescence development, formation of rachis branches such as primary rachis branches and secondary rachis branches, and spikelet specialisation (lateral and terminal spikelets). The molecular genetic basis of GNPP determination is complex, and it is regulated by numerous interlinked genes. In this review, panicle development and the determination of GNPP is described briefly, and GNPP-related genes that influence its determination are categorised according to their regulatory mechanisms. We introduce genes related to rachis branch development and their regulation of GNPP, genes related to phase transition (from rachis branch meristem to spikelet meristem) and their regulation of GNPP, and genes related to spikelet specialisation and their regulation of GNPP. In addition, we describe other GNPP-related genes and their regulation of GNPP. Research on GNPP determination suggests that it is possible to cultivate rice varieties with higher grain yield by modifying GNPP-related genes.
Physiology, Biochemistry, and Pharmacology of Transporters for Organic Cations
Authors: Ciarimboli Giuliano
Published: January 2021

This editorial summarizes the 13 scientific papers published in the Special Issue “Physiology, Biochemistry, and Pharmacology of Transporters for Organic Cations” of the International Journal of Molecular Sciences [...]
Single Administration of the T-Type Calcium Channel Enhancer SAK3 Reduces Oxidative Stress and Improves Cognition in Olfactory Bulbectomized Mice
Authors: Yuan Dian , Cheng An , Kawahata Ichiro , Izumi Hisanao , Xu Jing , Fukunaga Kohji
Published: January 2021

Alzheimer’s disease (AD), characterized by cognitive impairments, is considered to be one of the most widespread chronic neurodegenerative diseases worldwide. We recently introduced a novel therapeutic agent for AD treatment, the T-type calcium channel enhancer ethyl-8-methyl-2,4-dioxo-2-(piperidin-1-yl)-2H-spiro[cyclopentane-1,3-imidazo[1,2-a]pyridin]-2-ene-3-carboxylate (SAK3). SAK3 enhances calcium/calmodulin-dependent protein kinase II and proteasome activity, thereby promoting amyloid beta degradation in mice with AD. However, the antioxidative effects of SAK3 remain unclear. We investigated the antioxidative effects of SAK3 in olfactory bulbectomized mice (OBX mice), compared with the effects of donepezil as a positive control. As previously reported, single oral administration of both SAK3 (0.5 mg/kg, p.o.) and donepezil (1.0 mg/kg, p.o.) significantly improved cognitive and depressive behaviors in OBX mice. Single oral SAK3 administration markedly reduced 4-hydroxy-2-nonenal and nitrotyrosine protein levels in the hippocampus of OBX mice, which persisted until 1 week after administration. These effects are similar to those observed with donepezil therapy. Increased protein levels of oxidative stress markers were observed in the microglial cells, which were significantly rescued by SAK3 and donepezil. SAK3 could ameliorate oxidative stress in OBX mice, like donepezil, suggesting that the antioxidative effects of SAK3 and donepezil are among the neuroprotective mechanisms in AD pathogenesis.
Involvement of SUT1 and SUT2 Sugar Transporters in the Impairment of Sugar Transport and Changes in Phloem Exudate Contents in Phytoplasma-Infected Plants
Authors: Marco Federica De , Batailler Brigitte , Thorpe Michael R. , Razan Frédérique , Le Hir Rozenn , Vilaine Françoise , Bouchereau Alain , Martin-Magniette Marie-Laure , Eveillard Sandrine , Dinant Sylvie
Published: January 2021

Phytoplasmas inhabit phloem sieve elements and cause abnormal growth and altered sugar partitioning. However, how they interact with phloem functions is not clearly known. The phloem responses were investigated in tomatoes infected by “Candidatus Phytoplasma solani” at the beginning of the symptomatic stage, the first symptoms appearing in the newly emerged leaf at the stem apex. Antisense lines impaired in the phloem sucrose transporters SUT1 and SUT2 were included. In symptomatic sink leaves, leaf curling was associated with higher starch accumulation and the expression of defense genes. The analysis of leaf midribs of symptomatic leaves indicated that transcript levels for genes acting in the glycolysis and peroxisome metabolism differed from these in noninfected plants. The phytoplasma also multiplied in the three lower source leaves, even if it was not associated with the symptoms. In these leaves, the rate of phloem sucrose exudation was lower for infected plants. Metabolite profiling of phloem sap-enriched exudates revealed that glycolate and aspartate levels were affected by the infection. Their levels were also affected in the noninfected SUT1- and SUT2-antisense lines. The findings suggest the role of sugar transporters in the responses to infection and describe the consequences of impaired sugar transport on the primary metabolism.
Novel Tyrosine Kinase Targets in Urothelial Carcinoma
Authors: Torres-Jiménez Javier , Albarrán-Fernández Víctor , Pozas Javier , Román-Gil María San , Esteban-Villarrubia Jorge , Carrato Alfredo , Rosero Adriana , Grande Enrique , Alonso-Gordoa Teresa , Molina-Cerrillo Javier
Published: January 2021

Urothelial carcinoma represents one of the most prevalent types of cancer worldwide, and its incidence is expected to grow. Although the treatment of the advanced disease was based on chemotherapy for decades, the developments of different therapies, such as immune checkpoint inhibitors, antibody drug conjugates and tyrosine kinase inhibitors, are revolutionizing the therapeutic landscape of this tumor. This development coincides with the increasing knowledge of the pathogenesis and genetic alterations in urothelial carcinoma, from the non-muscle invasive setting to the metastatic one. The purpose of this article is to provide a comprehensive review of the different tyrosine kinase targets and their roles in the therapeutic scene of urothelial carcinoma.
Phosphodiesterase Type-5 Inhibitor Tadalafil Modulates Steroid Hormones Signaling in a Prostate Cancer Cell Line
Authors: Bimonte Viviana M. , Marampon Francesco , Antonioni Ambra , Fittipaldi Simona , Ferretti Elisabetta , Pestell Richard G. , Curreli Mariaignazia , Lenzi Andrea , Vitale Giovanni , Brunetti Antonio , Migliaccio Silvia , Aversa Antonio
Published: January 2021

Background: The androgen receptor (AR) plays a key role in normal prostate homeostasis and in prostate cancer (PCa) development, while the role of aromatase (Cyp19a1) is still unclear. We evaluated the effects of a treatment with Tadalafil (TAD) on both these proteins. Methods: Androgen-sensitive human PCa cell line (LnCAP) was incubated with/without TAD (10−6 M) and bicalutamide (BCT) (10−4 M) to evaluate a potential modulation on cell proliferation, protein and mRNA expression of Cyp19a, AR and estrogen receptor-β (ERβ), respectively. Results: TAD increased early AR nuclear translocation (p < 0.05, after 15 min of exposure), and increased AR transcriptional activity (p < 0.05) and protein expression (p < 0.05) after 24 h. Moreover, after 24 h this treatment upregulated Cyp19a1 and ERβ mRNA (p < 0.05 and p < 0.005 respectively) and led to an increase in protein expression of both after 48 h (p < 0.05). Interestingly, TAD counteracted Cyp19a1 stimulation induced by BCT (p < 0.05) but did not alter the effect induced by BCT on the AR protein expression. Conclusion: We demonstrate for the first time that TAD can significantly modulate AR expression and activity, Cyp19a1 and ERβ expression in PCa cells, suggesting a specific effect of these proteins. In addition, TAD potentiates the antiproliferative activity of BCT, opening a new clinical scenario in the treatment of PCa.
Systematic Identification of Protein Targets of Sub5 Using Saccharomyces cerevisiae Proteome Microarrays
Authors: Shah Pramod , Chen Chien-Sheng
Published: January 2021

Antimicrobial peptides (AMPs) are intensively studied in terms of alternative drugs. Sub5 is a synthetic 12-mer AMP with substitutions of five amino acids of bactenecin 2A (Bac2A), a linear-ized bactenecin variant of bovine. Sub5 is highly effective against fungi with an ability to trans-locate cell membrane, but its targets are unknown. Systematic analysis of Sub5 targets will facil-itate our understanding on its mechanism of action. In this study, we used high-throughput Saccharomyces cerevisiae proteome microarrays to explore the potential protein targets of Sub5. The screening results showed 128 potential protein targets of Sub5. Bioinformatics analysis of protein targets of Sub5 revealed significant gene ontology (GO) enrichment in actin related pro-cess of “actin filament-based process”, “actin filament organization”, “actin cortical patch or-ganization”, regulation of “actin filament bundle assembly”. Moreover, the other enriched cat-egories in GO enrichment mostly contained actin associate proteins. In total, 11 actin-associated proteins were identified in the protein targets of Sub5. Protein family (PFAM) enrichment anal-ysis shows protein domain enriched in actin binding, i.e., “Cytoskeletal-regulatory complex EF hand (helix E-loop-helix F motif)”. Being consistent with GO analysis, Search Tool for the Re-trieval of Interacting Genes/Proteins (STRING) analysis of the protein targets of Sub5 showed ac-tin network with involvement of 15 protein targets. Along with actin-network, STRING analysis showed protein–protein interaction network in ribonucleoprotein, transcription and translation, chromosome, histone, and ubiquitin related, DNA repair, and chaperone. Multiple Expression motifs for Motif Elicitation (MEME) suite provided a consensus binding motif of [ED][ED]EEE[ED][ED][ED][ED][ED], in total of 75 protein targets of Sub5. This motif was present in 9 out of 15 actin-related proteins identified among protein targets of Sub5.
Applications and Perspectives of Ultrasonic Multi-Gas Analysis with Simultaneous Flowmetry
Authors: Hallewell Gregory , Dingley John , Doubek Martin , Feuillassier Robin , Katunin Sergey , Nagai Koichi , Robinson David , Rozanov Alexandre , Williams David , Vacek Vaclav
Published: January 2021

We have developed ultrasonic instrumentation for simultaneous flow and composition measurement in a variety of gas mixtures. Flow and composition are respectively derived from measurements of the difference and average of sound transit times in opposite directions in a flowing process gas. We have developed a sound velocity-based algorithm to compensate for the effects of additional gases, allowing the concentrations of a pair of gases of primary interest to be acoustically measured on top of a varying baseline from ‘third party’ gases whose concentrations in the multi-gas mixture are measured by other means. Several instruments are used in the CERN ATLAS experiment. Three monitor C3F8, (R218), and CO2 coolant leaks into N2-purged environmental envelopes. Precision in molar concentration of better than 2 × 10−5 is routinely seen in mixtures of C3F8 in N2 in the presence of varying known concentrations of CO2. Further instruments monitor air ingress and C3F8 vapor flow (at high mass flows around 1.1 kg s−1) in the 60 kW thermosiphon C3F8 evaporative cooling recirculator. This instrumentation and analysis technique, targeting binary pairs of gases of interest in multi-gas mixtures, is promising for mixtures of anesthetic gases, particularly in the developing area of xenon anesthesia.
Could Chronic Idiopatic Intestinal Pseudo-Obstruction Be Related to Viral Infections?
Authors: Sinagra Emanuele , Pellegatta Gaia , Maida Marcello , Rossi Francesca , Conoscenti Giuseppe , Pallio Socrate , Alloro Rita , Raimondo Dario , Anderloni Andrea
Published: January 2021

Chronic idiopathic intestinal pseudo-obstruction (CIIPO) is a disease characterized by symptoms and signs of small bowel obstruction in the absence of displayable mechanical obstruction. Due to the known neuropathic capacity of several viruses, and their localization in the intestine, it has been hypothesized that such viruses could be involved in the pathogenesis of CIIPO. The most frequently involved viruses are John Cunningham virus, Herpesviridae, Flaviviruses, Epstein–Barr virus and Citomegalovirus. Therefore, the present narrative review aims to sum up some new perspectives in the etiology and pathophysiology of CIIPO.
Gadolinium-Based Contrast Media Nephrotoxicity in Kidney Impairment: The Physio-Pathological Conditions for the Perfect Murder
Authors: Martino Francesca , Amici Gianpaolo , Rosner Mitchell , Ronco Claudio , Novara Giacomo
Published: January 2021

Gadolinium-based contrast media (GBCM) toxicity in patients with kidney disease is a concern for the possible development of systemic nephrogenic fibrosis and possible renal complications. This review focuses on the pathological mechanisms underlying the potential kidney toxicity of gadolinium. Gadolinium, as a free compound (Gd3+), is highly toxic in humans because it competes with divalent calcium (Ca2+) and magnesium (Mg2+) ions, interfering in some relevant biologic processes. Its toxicity is blunted by the complexing of Gd3+ with a carrier, allowing its use in magnetic resonance imaging. The binding reaction between gadolinium and a carrier is thermodynamically reversible. Consequently, under some conditions, gadolinium can be released in the interstitial space as a free Gd3+ compound with the possibility of toxicity. Other metals such as iron, copper, and calcium can interfere with the binding between gadolinium and its carrier because they compete for the same binding site. This process is known as transmetallation. In patients with kidney impairment, conditions such as low clearance of the Gd-carrier complex, acid-base derangements, and high serum phosphorous can increase the presence of free Gd3+, leading to a higher risk for toxicity.
Biomimetics and Composite Materials toward Efficient Mobility: A Review
Authors: Boaretto Joel , Fotouhi Mohammad , Tende Eduardo , Aver Gustavo Francisco , Marcon Victoria Rafaela Ritzel , Cordeiro Guilherme Luís , Bergmann Carlos Pérez , Vannucchi de Camargo Felipe
Published: January 2021

The development of new materials has always been strictly related to the rise of new technologies and progressively efficient systems. However, cutting-edge materials might not be enough to ensure the effectiveness of a given product if the design guidelines used do not favor the specific advantages of this material. Polymeric composites are known for their excellent mechanical properties, but current manufacturing techniques and the relatively narrow expertise in the field amongst engineers impose the challenge to provide the most suitable designs to certain applications. Bio-inspired designs, supported by thousands of years of evolution of nature, have shown to be extremely profitable tools for the design of optimized yet structurally complex shapes in which the tailoring aspect of polymeric composites perfectly fit. Bearing in mind the current but old-fashioned designs of auto-parts and vehicles built with metals with little or no topological optimization, the present work addresses how biomimicry is being applied in the mobility industry nowadays to provide lightweight structures and efficient designs. A general overview of biomimicry is made regarding vehicles, approaching how the use of composite materials has already contributed to successful cases.
A Systematic Review of Strength and Conditioning Protocols for Improving Neck Strength and Reducing Concussion Incidence and Impact Injury Risk in Collision Sports; Is There Evidence?
Authors: Daly Ed , Pearce Alan J. , Ryan Lisa
Published: January 2021

The objective of this systematic literature review was to evaluate the evidence regarding the development of neck strength in reducing concussion and cervical spine injuries in adult amateur and professional sport populations. PubMed, CINAHL, Science Direct, and Web of Science databases were searched systematically. The criteria for inclusion in the review were as follows: (1) a human adult (≥18 or above); (2) involved in amateur, semi-professional, or professional sports; (3) sports included involved collisions with other humans, apparatus or the environment; (4) interventions included pre- and post-neck muscle strength measures or neck stability measures; (5) outcomes included effects on increasing neck strength in participants and/or injury incidence. Database searches identified 2462 articles. Following title, abstract, and full paper screening, three papers were eligible for inclusion. All of the papers reported information from male participants, two were focused on rugby union, and one on American football. Two of the included studies found a significant improvement in isometric neck strength following intervention. None of the studies reported any impact of neck strengthening exercises on cervical spine injuries. This review has shown that there is currently a lack of evidence to support the use of neck strengthening interventions in reducing impact injury risk in adult populations who participate in sport.
Verification and Validation of CFD Based Form Factors as a Combined CFD/EFD Method
Authors: Korkmaz Kadir Burak , Werner Sofia , Bensow Rickard
Published: January 2021

Predicting the propulsive power of ships with high accuracy still remains a challenge. Well established practices in the 1978 ITTC Power Prediction method have been questioned such as the form factor approach and its determination method. This paper investigates the possibility to improve the power predictions by the introduction of a combined CFD/EFD Method where the experimental determination of form factor is replaced by double body RANS computations. Following the Quality Assurance Procedure proposed by ITTC, a best practice guideline has been derived for the CFD based form factor determination method by applying systematic variations to the CFD set-ups. Following the verification and validation of the CFD based form factor method in model scale, the full scale speed-power-rpm relations between large number of speed trials and full scale predictions using the CFD based form factors in combination with ITTC-57 line and numerical friction lines are investigated. It is observed that the usage of CFD based form factors improves the predictions in general and no deterioration is noted within the limits of this study. Therefore, the combination of EFD and CFD is expected to provide immediate improvements to the 1978 ITTC Performance Prediction Method.
A Proposed Land Exchange Algorithm for Eliminating the External Plot Patchwork
Authors: Stręk Żanna , Leń Przemysław , Wójcik-Leń Justyna , Postek Paweł , Mika Monika , Dawid Leszek
Published: January 2021

In many countries of the world, rural areas are characterized by a defective spatial structure of agricultural land. The most frequent defects are large fragmentation and distribution of farmland. The fragmentation of land has been an issue widely described by many authors throughout the world. The problem of the distribution of land owned by individual farmers is slightly different, since due to the complexity of the problem this issue was not widely explored in Poland (plot patchwork) or in other countries of Europe and the world. Land fragmentation and distribution of plots in rural areas has a negative effect on the profitability and efficiency of agricultural production. Land consolidation and exchange is an operation facilitating spatial structure improvement. The authors attempted to develop a universal land exchange algorithm for eliminating the external plot patchwork. As it turns out, so far no land exchange algorithm has been developed. Specific analyses were carried out in Puchaczów commune, county of Łęczna, Lublin voivodeship in the eastern part of Poland, covering an area of 6907.80 ha, split into 15,211 plots. The chequerboard arrays method was used. The publication presents the algorithm and its practical application using a test sample. A result of the studies is a proposal concerning the exchange of land between landowners in the villages of the commune of Puchaczów. Using the algorithm, the area of individual lands in the commune, after the exchange, will increase by 172.09 ha, which is 2.5% for the area of individual lands, and 1.9% for the commune.
Definition of Damage Indices for Railway Axle Bearings: Results of Long-Lasting Tests
Authors: Pennacchi Paolo , Chatterton Steven , Vania Andrea , Massocchi Davide
Published: January 2021

The European Rail Transport System has set goals of improving the reliability, availability, durability, high-speed capacity, and maintenance. In this context, the condition monitoring approach is of fundamental importance for all components of the “train” system. The authors have long been in the diagnostics of the railway traction system, with a focus on rolling element bearings. In this paper, signal processing techniques, already developed in the past and experimentally validated in the railway field, are applied to vibration data for the definition of damage indices for railway axle bearings. This allows to evaluate the “status” of the bearing and the monitoring of the evolution of any damage that may occur. The experimental data were obtained by means of a test-rig built according to EN 12082:2017 and following the test specifications of the same standard.
New Series Solution of the Caputo Fractional Ambartsumian Delay Differential Equationation by Mittag-Leffler Functions
Authors: Alharbi Weam , Hristova Snezhana
Published: January 2021

The fractional generalization of the Ambartsumian delay equation with Caputo’s fractional derivative is considered. The Ambartsumian delay equation is very difficult to be solved neither in the case of ordinary derivatives nor in the case of fractional derivatives. In this paper we combine the Laplace transform with the Adomian decomposition method to solve the studied equation. The exact solution is obtained as a series which terms are expressed by the Mittag-Leffler functions. The advantage of the present approach over the known in the literature ones is discussed.
Chaotic Multi-Objective Simulated Annealing and Threshold Accepting for Job Shop Scheduling Problem
Authors: Frausto-Solis Juan , Hernández-Ramírez Leonor , Castilla-Valdez Guadalupe , González-Barbosa Juan J. , Sánchez-Hernández Juan P.
Published: January 2021

The Job Shop Scheduling Problem (JSSP) has enormous industrial applicability. This problem refers to a set of jobs that should be processed in a specific order using a set of machines. For the single-objective optimization JSSP problem, Simulated Annealing is among the best algorithms. However, in Multi-Objective JSSP (MOJSSP), these algorithms have barely been analyzed, and the Threshold Accepting Algorithm has not been published for this problem. It is worth mentioning that the researchers in this area have not reported studies with more than three objectives, and the number of metrics they used to measure their performance is less than two or three. In this paper, we present two MOJSSP metaheuristics based on Simulated Annealing: Chaotic Multi-Objective Simulated Annealing (CMOSA) and Chaotic Multi-Objective Threshold Accepting (CMOTA). We developed these algorithms to minimize three objective functions and compared them using the HV metric with the recently published algorithms, MOMARLA, MOPSO, CMOEA, and SPEA. The best algorithm is CMOSA (HV of 0.76), followed by MOMARLA and CMOTA (with HV of 0.68), and MOPSO (with HV of 0.54). In addition, we show a complexity comparison of these algorithms, showing that CMOSA, CMOTA, and MOMARLA have a similar complexity class, followed by MOPSO.
A Microencapsulation Method for Delivering Tetrodotoxin to Bivalves to Investigate Uptake and Accumulation
Authors: Biessy Laura , Smith Kirsty F. , Wood Susanna A. , Tidy Annabel , van Ginkel Roel , Bowater Joel R. D. , Hawes Ian
Published: January 2021

Most marine biotoxins are produced by microalgae. The neurotoxin tetrodotoxin (TTX) has been reported in many seafood species worldwide but its source is unknown, making accumulation and depuration studies in shellfish difficult. Tetrodotoxin is a water-soluble toxin and cannot be directly ingested by shellfish. In the present study, a method was developed which involved binding TTX to solid particles of humic acid and encapsulating them in agar-gelatin capsules. A controlled quantity of TTX-containing microcapsules (size range 20–280 μm) was fed to Paphies australis, a bivalve known to accumulate TTX in the wild. The TTX-containing microcapsules were fed to P. australis every second day for 13 days. Ten P. australis (including five controls fed non-toxic microalgae) were harvested after 7 days and ten after 13 days. Paphies australis accumulated TTX, reaching concentrations of up to 103 µg kg−1 by day 13, exceeding the European Food Safety Authority recommended concentration of 44 μg kg−1 in shellfish. This novel method will allow future studies to explore the effects, accumulation and depuration rates of TTX in different animals and document how it is transferred through food webs.