bluuebunny/crossref_metadata_2025 huggingface dataset:
Extracorporeal Shock Wave Therapy Salvages Critical Limb Ischemia in B6 Mice through Upregulating Cell Proliferation Signaling and Angiogenesis
Authors: Sung Pei-Hsun , Yin Tsung-Cheng , Chai Han-Tan , Chiang John Y. , Chen Chih-Hung , Huang Chi-Ruei , Yip Hon-Kan
Published: January 2022

(1) This study tests hypothesis whether extracorporeal shock wave (ECSW) therapy effectively salvages mouse critical limb ischemia (CLI). In vitro result demonstrated that the angiogenesis parameters (i.e., tubular length/cluster/network formation) and protein expressions of EGFR/VEGFR2/RAS/c-Raf/MEK/ERK/VEGF/p-PI3K/p-Akt/p-m-TOR were significantly and progressively increased with stepwise augmentation of ECSW energy (0.1/0.14/0.20 mJ/mm2/140 impulses). On the other hand, they were suppressed by administration of Avastin (20 μM). Adult male B6 mice (n = 24) were equally categorized into group 1 (sham-operated control), group 2 (CLI), group 3 [CLI + ECSW (0.12 mJ/mm2/120 impulses/at days 1/3/7 after CLI induction)] and group 4 [CLI + ECSW (0.12 mJ/mm2/120 impulses) + Avastin (1 mg/intramuscular-injection)] at days 1/3/7 after CLI induction] and quadriceps were harvested by day 14. The laser Doppler result showed that the ratio of left (ischemia) to right (normal) limb blood flow was highest in group 1, lowest in group 2, and significantly higher in group 3 than in group 4 by days 7/14 after the CLI procedure (p < 0.0001). The protein expressions of cell proliferation/migration/angiogenesis receptors (EGFR/VEGFR2), angiogenesis biomarkers (VEGF/CXCR4/SDF-1) and cell proliferation/growth/survival (Ras/c-Raf/MEK/ERK)/(PI3K/Akt/m-TOR) and cell motility/proliferation (p-FAK/p-Scr) signaling biomarkers were significantly higher in group 3 than in groups 1/2/4, and significantly lower in group 1 than in groups 2/4, but they did not show a difference between groups 2 and 4 (all p < 0.001). The small vessel density and cellular levels of endothelial cell surface marker (CD31+) exhibited an identical pattern of blood flow, whereas the angiogenesis (CXCR4+/VEGF+) displayed an identical pattern of VEGFR2 among the groups (all p < 0.0001). The in vitro and in vivo studies found ECSW salvaged the CLI mainly through upregulating Ras-Raf-MEK/ERK/cell motility, cell proliferation/growth pathways and angiogenesis.
Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)
Authors: Tavakoli Shima , Kisiel Marta A. , Biedermann Thomas , Klar Agnes S.
Published: January 2022

The immune system has a crucial role in skin wound healing and the application of specific cell-laden immunomodulating biomaterials emerged as a possible treatment option to drive skin tissue regeneration. Cell-laden tissue-engineered skin substitutes have the ability to activate immune pathways, even in the absence of other immune-stimulating signals. In particular, mesenchymal stem cells with their immunomodulatory properties can create a specific immune microenvironment to reduce inflammation, scarring, and support skin regeneration. This review presents an overview of current wound care techniques including skin tissue engineering and biomaterials as a novel and promising approach. We highlight the plasticity and different roles of immune cells, in particular macrophages during various stages of skin wound healing. These aspects are pivotal to promote the regeneration of nonhealing wounds such as ulcers in diabetic patients. We believe that a better understanding of the intrinsic immunomodulatory features of stem cells in implantable skin substitutes will lead to new translational opportunities. This, in turn, will improve skin tissue engineering and regenerative medicine applications.
Nonsense-Mediated mRNA Decay, a Finely Regulated Mechanism
Authors: Lejeune Fabrice
Published: January 2022

Nonsense-mediated mRNA decay (NMD) is both a mechanism for rapidly eliminating mRNAs carrying a premature termination codon and a pathway that regulates many genes. This implies that NMD must be subject to regulation in order to allow, under certain physiological conditions, the expression of genes that are normally repressed by NMD. Therapeutically, it might be interesting to express certain NMD-repressed genes or to allow the synthesis of functional truncated proteins. Developing such approaches will require a good understanding of NMD regulation. This review describes the different levels of this regulation in human cells.
Combinatorial Virtual Screening Revealed a Novel Scaffold for TNKS Inhibition to Combat Colorectal Cancer
Authors: Chang Chun-Chun , Pan Sheng-Feng , Wu Min-Huang , Cheng Chun-Tse , Su Yan-Rui , Jiang Shinn-Jong , Hsu Hao-Jen
Published: January 2022

The abnormal Wnt signaling pathway leads to a high expression of β-catenin, which causes several types of cancer, particularly colorectal cancer (CRC). The inhibition of tankyrase (TNKS) activity can reduce cancer cell growth, invasion, and resistance to treatment by blocking the Wnt signaling pathway. A pharmacophore search and pharmacophore docking were performed to identify potential TNKS inhibitors in the training databases. The weighted MM/PBSA binding free energy of the docking model was calculated to rank the databases. The reranked results indicated that 26.98% of TNKS inhibitors that were present in the top 5% of compounds in the database and near an ideal value ranked 28.57%. The National Cancer Institute database was selected for formal virtual screening, and 11 potential TNKS inhibitors were identified. An enzyme-based experiment was performed to demonstrate that of the 11 potential TNKS inhibitors, NSC295092 and NSC319963 had the most potential. Finally, Wnt pathway analysis was performed through a cell-based assay, which indicated that NSC319963 is the most likely TNKS inhibitor (pIC50 = 5.59). The antiproliferation assay demonstrated that NSC319963 can decrease colorectal cancer cell growth; therefore, the proposed method successfully identified a novel TNKS inhibitor that can alleviate CRC.
Sensing of C-Reactive Protein Using an Extended-Gate Field-Effect Transistor with a Tungsten Disulfide-Doped Peptide-Imprinted Conductive Polymer Coating
Authors: Liu Kai-Hsi , Lin Hung-Yin , Thomas James L. , Chen Chen-Yuan , Chen Yen-Ting , Chen Chuen-Yau , Yang Chien-Hsin , Lee Mei-Hwa
Published: January 2022

C-reactive protein (CRP) is a non-specific biomarker of inflammation and may be associated with cardiovascular disease. In recent studies, systemic inflammatory responses have also been observed in cases of coronavirus disease 2019 (COVID-19). Molecularly imprinted polymers (MIPs) have been developed to replace natural antibodies with polymeric materials that have low cost and high stability and could thus be suitable for use in a home-care system. In this work, a MIP-based electrochemical sensing system for measuring CRP was developed. Such a system can be integrated with microfluidics and electronics for lab-on-a-chip technology. MIP composition was optimized using various imprinting template (CRP peptide) concentrations. Tungsten disulfide (WS2) was doped into the MIPs. Doping not only enhances the electrochemical response accompanying the recognition of the template molecules but also raises the top of the sensing range from 1.0 pg/mL to 1.0 ng/mL of the imprinted peptide. The calibration curve of the WS2-doped peptide-imprinted polymer-coated electrodes in the extended-gate field-effect transistor platform was obtained and used for the measurement of CRP concentration in real human serum.
Exploring Visualisation Methodology of Landscape Design on Rural Tourism in China
Authors: Wang Weijia , Watanabe Makoto , Ono Kenta , Zhou Donghong
Published: January 2022

Rural tourism has become a hot topic in China in the context of the nation’s rural revitalisation. Rural tourism allows tourists to experience local life and promotes local economic development. However, there is considerable controversy over the landscape design of ancient Chinese villages. Many problems, such as how to design and protect the landscape of these ancient villages and how to improve the tourist experience, are not resolved. For our research object, we selected the ancient Gaotiankeng Village in Kaihua County, Zhejiang Province. Using questionnaires, image interviews, and some user experience techniques such as mental maps, we collected user experience data by assessing design cases. The visualisation method presented a wide range of experience in the landscape and planning field. This study primarily used computer image processing, image entropy calculation, and colour mapping to process the data. A visualisation framework was defined to highlight the landscape aesthetics, landscape service, and tourists’ emotion. The results indicated the relationship of three elements. The objective of our study was to develop a method of landscape design and planning that can effectively enhance tourists’ experience and provide practical suggestions for rural landscapes and relatively better services.
Development of a Screening Method for Sulfamethoxazole in Environmental Water by Digital Colorimetry Using a Mobile Device
Authors: Peixoto Patrícia S. , Carvalho Pedro H. , Machado Ana , Barreiros Luisa , Bordalo Adriano A. , Oliveira Hélder P. , Segundo Marcela A.
Published: January 2022

Antibiotic resistance is a major health concern of the 21st century. The misuse of antibiotics over the years has led to their increasing presence in the environment, particularly in water resources, which can exacerbate the transmission of resistance genes and facilitate the emergence of resistant microorganisms. The objective of the present work is to develop a chemosensor for screening of sulfonamides in environmental waters, targeting sulfamethoxazole as the model analyte. The methodology was based on the retention of sulfamethoxazole in disks containing polystyrene divinylbenzene sulfonated sorbent particles and reaction with p-dimethylaminocinnamaldehyde, followed by colorimetric detection using a computer-vision algorithm. Several color spaces (RGB, HSV and CIELAB) were evaluated, with the coordinate a_star, from the CIELAB color space, providing the highest sensitivity. Moreover, in order to avoid possible errors due to variations in illumination, a color palette is included in the picture of the analytical disk, and a correction using the a_star value from one of the color patches is proposed. The methodology presented recoveries of 82–101% at 0.1 µg and 0.5 µg of sulfamethoxazole (25 mL), providing a detection limit of 0.08 µg and a quantification limit of 0.26 µg. As a proof of concept, application to in-field analysis was successfully implemented.
In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device
Authors: Kim Jae-Hyung , Lee Young-Ju , Ahn Yong-Jin , Kim Minyoung , Lee Gi-Ja
Published: January 2022

In this study, a paper-integrated analytical device that combined a paper-based colorimetric assay with a paper-based cell culture platform was developed for the in situ detection of hydrogen sulfide (H2S) in three-dimensional (3D)-cultured, live prostate cancer cells. Two kinds of paper substrates were fabricated using a simple wax-printing methodology to form the cell culture and detection zones, respectively. LNCaP cells were seeded directly on the paper substrate and grown in the paper-integrated analytical device. The cell viability and H2S production of LNCaP cells were assessed using a simple water-soluble tetrazolium salt colorimetric assay and H2S-sensing paper, respectively. The H2S-sensing paper showed good sensitivity (sensitivity: 6.12 blue channel intensity/μM H2S, R2 = 0.994) and a limit of quantification of 1.08 μM. As a result, we successfully measured changes in endogenous H2S production in 3D-cultured, live LNCaP cells within the paper-integrated analytical device while varying the duration of incubation and substrate concentration (L-cysteine). This paper-integrated analytical device can provide a simple and effective method to investigate H2S signaling pathways and drug screening in a 3D culture model.
Assessment of Public Special Education Teachers Training Needs on Evidence-Based Practice for Students with Autism Spectrum Disorders in Spain
Authors: Larraceleta Aitor , Castejón Luis , Iglesias-García María-Teresa , Núñez José Carlos
Published: January 2022

Over decades, the concern for the quality of psychoeducational practices for students with autism spectrum disorders has led to study to what extent are evidence-based educational methods disseminated among teachers. The purpose of this cross-sectional study, taking as reference Hsiao and Sorensen’s previous research, was to identify through a survey to what extent social-communication evidence-based practices for these students were provided in teacher education and in-service training programs, in a sample of 108 special education teachers from Spain, and to compare these results with Hsiao and Sorensen’s. Overall, more than 70% of the teachers reported that evidence-based practices in their teacher education programs (87.6%) and in-service training programs (73.6%) were never taught or mentioned incidentally. Finally, a higher percentage of addressing on each practice (i.e., mentioned and discussed or mentioned and taught through direct instruction) is shown in the sample of American teachers compared to the Spaniards, in both training paths.
Comparison of Hospital Consultation and Summer Camp Lifestyle Intervention Programs for Sustained Body Weight Loss in Overweight/Obese Greek Children
Authors: Papageorgiou Anna L. , Efthymiou Vasiliki , Giannouli Aikaterini , Xekouki Paraskevi , Kranioti Christina C. , Chrousos George P.
Published: January 2022

Two lifestyle intervention programs of a health initiative named “Evrostia” were conducted at (a) an outpatient obesity clinic of a children’s hospital and (b) summer camp (SC), respectively. Thirty overweight/obese children were randomly selected to participate in each intervention arm to assess the efficacy of the SC intervention and its possible superiority over usual hospital consultation (HC) practice. There was a statistically significant decrease in body weight (BW), and body mass index (BMI) in both programs. A higher duration of reduced BW was observed in the SC compared to HC intervention. Regarding the nutritional behavior, there was a significant increase in the consumption of breakfast, fruit and vegetables, and a reduction in the consumption of beverages and sweets in the SC group. A significant increase in the hours of weekly physical activity was also observed in children of the SC program. The comparison between the two lifestyle intervention programs showed that the SC program improved nutritional behaviors and physical activity and promoted longer preservation of BW loss than that of the HC program. Thus, the holistic and experiential approach of the SC program was more successful in the treatment of overweight and obesity in children than a conventional HC program.
Early Developmental Signs in Children with Autism Spectrum Disorder: Results from the Japan Environment and Children’s Study
Authors: Shimomura Hideki , Hasunuma Hideki , Tokunaga Sachi , Taniguchi Yohei , Taniguchi Naoko , Fujino Tetsuro , Utsunomiya Takeshi , Tanaka Yasuhiko , Tokuda Narumi , Okuda Masumi , Shima Masayuki , Takeshima Yasuhiro ,   The Japan Environment and Children’s Study (JECS) Group
Published: January 2022

Autism spectrum disorder (ASD) is a developmental disability in early childhood. Early identification and intervention in children with ASD are essential for children and their families. This study aimed to identify the earliest signs of ASD. Using a large cohort including data from 104,062 fetal records in the Japan Environment and Children’s Study, we examined the Ages and Stages Questionnaires® (ASQ-3TM) scores of children with and without ASD. The ASQ-3 comprises five domains: communication, gross motor, fine motor, problem solving, and personal-social. The ASQ-3 scores were obtained at ages 6 months, 1 year, and 3 years. There were 64,501 children with available ASQ-3 data. The number of children diagnosed with ASD was 188 (0.29%) at 3 years of age. The highest relative risk (RR) for any domain below the monitoring score at 6 months was in the communication (RR 1.90, 95% CI 1.29–2.78, p = 0.0041), followed by fine motor (RR 1.50, 95% CI 1.28–1.76, p < 0.0001) domain. A low ASQ-3 score in the communication domain at 6 months was related to an ASD diagnosis at 3 years of age. The ASQ-3 score at 6 months can contribute to the early identification of and intervention for ASD.
Historic Climate in Heritage Building and Standard 15757: Proposal for a Common Nomenclature
Authors: Fabbri Kristian
Published: January 2022

Research on the relationship between microclimate and heritage buildings or historic buildings has increased dramatically in the last few decades. Research has focused on indoor climate or indoor microclimate or the environment or micro-environment, and the field of these studies regards several variables, physical—air temperature, air speed, relative humidity—or chemical, dust, CO2, pollution, etc., all of which can have an effect or damage buildings or artifacts inside buildings. Moreover, all these variables should be monitored in a monitoring campaign following the standard EN 15757; in spite of this, scientific literature contains mistakes with regard to the words and objects of study. In this short contribution, the author proposes a common nomenclature in the research field of climate and microclimate in heritage buildings and heritage artifacts. A new nomenclature should be useful for the community of heritage scientists working on preventive measures to distinguish between climate and environment, or the object of study, e.g., the room (wall, wood structure, fresco, etc.) where the artifacts are or the air around them (painting, canvas, statue, piece of furniture, documents, books, etc.).
Diurnal Extrema Timing—A New Climatological Parameter?
Authors: Žaknić-Ćatović Ana , Gough William A.
Published: January 2022

We address the following question: Are turning points of daily air temperature function a piece of relevant climatological information worth recording and analyzing? Diurnal Extrema Timing (DET) are daily occurrence times of air temperature minimum and maximum. Although unrecognized and unrecorded as a meteorological variable, the exact timing of daily temperature extrema plays a crucial role in the characterization of air temperature variability. In this study, we introduce the DET concept and assess the plausibility of this potential parameter in detecting temperature extrema timing changes. Conceptualization of the DET parameter has, for a primary goal, the supplementation of vital spatial information to the daily measurements of air temperature extrema. The elementary analysis of annual trends of daily DET examines the significance of this parameter in describing changes in the time domain of air temperature variability. The introduction of the new Climate Parameter Sensitivity Index (CPSI) for evaluating the susceptibility of climate parameters to climate change directs attention to the importance of the systematic acquisition of the timing of daily extrema in climate observations. The results of this study reveal the timing of daily air temperature maximum as the most vulnerable to climate change among temperature and timing extrema indices.
On Numerical Modeling of Thermal Performance Enhancementof a Heat Thermal Energy Storage System Using a Phase Change Material and a Porous Foam
Authors: Mabrouk Riheb , Naji Hassane , Dhahri Hacen , Younsi Zouhir
Published: January 2022

In this investigation, a comprehensive numerical analysis of the flow involved in an open-ended straight channel fully filled with a porous metal foam saturated and a phase change material (paraffin) has been performed using a single relaxation time lattice Boltzmann method (SRT-LBM) at the representative elementary volume (REV) scale. The enthalpy-based approach with three density functions has been employed to cope with the governing equations under the local thermal non-equilibrium (LTNE) condition. The in-house code has been validated through a comparison with a previous case in literature. The pore per inch density (10≤PPI≤60) and porosity (0.7≤ε≤0.9) effects of the metal structure were analyzed during melting/solidifying phenomena at two Reynolds numbers (Re = 200 and 400). The relevant findings are discussed for the LTNE intensity and the entropy generation rate (Ns). Through the simulations, the LTNE hypothesis turned out to be secure and valid. In addition, it is maximum for small PPI value (=10) whatever the parameters deemed. On the other hand, high porosity (=0.9) is advised to reduce the system’s irreversibility. However, at a moderate Re (=200), a small PPI (=10) would be appropriate to mitigate the system irreversibility during the charging case, while a large value (PPI = 60) might be advised for the discharging case. In this context, it can be stated that during the melting period, low porosity (=0.7) with low PPI (=10) improves thermal performance, reduces the system irreversibility and speeds up the melting rate, while for high porosity (=0.9), a moderate PPI (=30) should be used during the melting process to achieve an optimal system.
Morphea Profunda with Tertiary Lymphoid Follicles: Description of Two Cases and Review of the Literature
Authors: Cassisa Angelo , Vannucchi Margherita
Published: January 2022

Morphea profunda or subcutaneous (deep) morphea is a variant of localized morphea, characterized by one or more ill-defined, deep sclerotic plaque. Preferential sites are the abdomen, trunk, sacral area, or extremities. The presence of hyperplastic lymphoid follicles in the context of the sclerotic bands of morphea is rarely described. Localized scleroderma is sustained by a profibrotic inflammatory profile. Transforming growth factor-β (TGF-β), an imbalance between functional subclasses of T-lymphocytes (innate immune cells) has a role in activate collagen deposition. In this case report, we present two cases of morphea profunda with lymphoid follicular hyperplasia. A systematic review of the literature on the pathophysiology of localized scleroderma is also presented, with particular reference to the presence of lymphoid structures.
Hidden Dissipation and Irreversibility in Maxwell’s Demon
Authors: Fontana Paul W.
Published: January 2022

Maxwell’s demon is an entity in a 150-year-old thought experiment that paradoxically appears to violate the second law of thermodynamics by reducing entropy without doing work. It has increasingly practical implications as advances in nanomachinery produce devices that push the thermodynamic limits imposed by the second law. A well-known explanation claiming that information erasure restores second law compliance fails to resolve the paradox because it assumes the second law a priori, and does not predict irreversibility. Instead, a purely mechanical resolution that does not require information theory is presented. The transport fluxes of mass, momentum, and energy involved in the demon’s operation are analyzed and show that they imply “hidden” external work and dissipation. Computing the dissipation leads to a new lower bound on entropy production by the demon. It is strictly positive in all nontrivial cases, providing a more stringent limit than the second law and implying intrinsic thermodynamic irreversibility. The thermodynamic irreversibility is linked with mechanical irreversibility resulting from the spatial asymmetry of the demon’s speed selection criteria, indicating one mechanism by which macroscopic irreversibility may emerge from microscopic dynamics.
University Students’ Classroom Emotional Climate and Attitudes during and after COVID-19 Lockdown
Authors: McLure Felicity I. , Koul Rekha B. , Fraser Barry J.
Published: January 2022

With the advent of COVID-19, universities around the world have been forced to move to a fully online mode of delivery because of lockdown policies. This led to a flurry of studies into issues such as internet access, student attitudes to online learning and mental health during lockdown. However, researchers need a validated survey for assessing the classroom emotional climate and student attitudes towards learning in universities that can be used for online, face-to-face or blended delivery. Such a survey could be used to illuminate students’ perceptions of the experiences that make up learning at university level, in terms of such factors as care from teachers, collaboration and motivation. In this article, we report the validation of a University Classroom Emotional Climate (UCEC) questionnaire and an Attitudes to Learning scale, as well as their use in comparing the classroom emotional climate and attitudes during COVID-19 lockdown (fully online delivery) with post-lockdown (mixed-mode delivery). Female students experienced the post-lockdown condition significantly more positively than during lockdown for all scales except Care, while the only significant difference for males between the during and post-lockdown was their choice to engage with learning (Control) and the degree of Challenge that they found with the learning materials.
The Potential to Build Collective Capacity for Organisational Learning in the Context of Teachers’ Use of Digital Technology for School Improvement
Authors: Davis-Singaravelu Susila
Published: January 2022

This article considers how digital spaces focused on whole school improvement combined with supportive leadership may be mobilised towards building collective capacity for evidence-informed practice and organisational learning. This topic originated from a qualitative, multi-method design-based research (DBR) project that studied practitioners’ use of an online resource for primary school practitioners called Pathways for school improvement, designed by Oxford University Press (OUP). Semi-structured interviews, participant observations and a documentary analysis were conducted with teachers and senior leaders in five primary schools across England between 2014 and 2016. Connections were made with the dynamic approach to school improvement (DASI) that encourages practitioners to systematically engage with a variety of evidence in their reflections and efforts to design school and classroom improvement strategies. Pathways’ four-step system and series of systematised tasks under each step seemed to provide opportunities for practitioners to explore elements of theory and practice in conjunction with empirical and pupil performance data, and potentially guide them through how to collaborate with others in developing specific whole school approaches to improvement. Opportunities and challenges in developing collective capacity for improvement are also explored.
Exergy Analysis of Alternative Configurations of Biomass-Based Light Olefin Production System with a Combined-Cycle Scheme via Methanol Intermediate
Authors: Li Yuping , Ye Maolin , Tan Fenghua , Wang Chenguang , Long Jinxing
Published: January 2022

Thermodynamic performance of three conceptual systems for biomass-derived olefin production with electricity cogeneration was studied and compared via exergy analysis at the levels of system, subsystem and operation unit. The base case was composed of the subsystems of gasification, raw fuel gas adjustment, methanol/light olefin synthesis and steam & power generation, etc. The power case and fuel case were designed as the combustion of a fraction of gasification gas to increase power generation and the recycle of a fraction of synthesis tail gas to increase olefin production, respectively. It was found that the subsystems of gasification and steam & power generation contribute ca. 80% of overall exergy destruction for each case, of which gasifier and combustor are the main exergy destruction sources, due to the corresponding chemical exergy degrading of biomass and fuel gas. The low efficiency of 33.1% for the power case could be attributed to the significant irreversibility of the combustor, economizer, and condenser in the combined-cycle subsystem. The effect of the tail gas recycle ratio, moisture content of feedstock, and biomass type was also investigated to enhance system exergy performance, which could be achieved by high recycle ratio, using dry biomass and the feedstock with high carbon content. High system efficiency of 38.9% was obtained when oil palm shell was used, which was 31.7% for rice husk due to its low carbon content.
Prediction of Oxidation Stability of Biodiesel Derived from Waste and Refined Vegetable Oils by Statistical Approaches
Authors: Çamur Hüseyin , Al-Ani Ahmed Muayad Rashid
Published: January 2022

The oxidation stability (OX) of the biodiesel is an essential parameter mainly during storage, which reduces the quality of the biodiesel, thus affecting the engine performance. Moreover, many factors affect oxidation stability. Therefore, determining the most significant parameter is essential for achieving accurate predictions. In this paper, an empirical equation (Poisson Regression Model (PRM)), machine learning models (Multilayer Feed-Forward Neural Network (MFFNN), Cascade Feed-forward Neural Network (CFNN), Radial Basis Neural Network (RBFNN), and Elman neural network (ENN)) with various combinations of input parameters are utilized and employed to identify the most relevant parameters for prediction of the oxidation stability of biodiesel. This study measured the physicochemical properties of 39 samples of waste frying methyl ester and their blends with various percentages of palm biodiesel and refined canola biodiesel. To this aim, 14 parameters including concentration amount of WFME (X1), PME (X2), and RCME (X3) in the mixture, kinematic viscosity (KV) at 40 °C, density at 15 °C (D), cloud point (CP), pour point (PP), the estimation value of the sum of the saturated (∑SFAMs), monounsaturated (∑MUFAMs), polyunsaturated (∑PUFAMs), degree of unsaturation (DU), long-chain saturated factor (LCSF), very-long-chain fatty acid (VLCFA), and ratio (∑MUFAMs+∑PUFAMs∑SFAMs) fatty acid composition were considered. The results demonstrated that the RBFNN model with the combination of X1, X2, X3, ∑SFAMs, ∑MUFAMs, ∑PUFAMs. VLCFA, DU, LCSF, ∑MUFAMs+∑PUFAMs∑SFAMs, KV, and D has the lowest value of root mean squared error and mean absolute error. In the end, the results demonstrated that the RBFNN model performed well and presented high accuracy in estimating the value of OX for the biodiesel samples compared to PRM, MFFNN, CFNN, and ENN.
Selection of Employees for Performing Work Activities in Currently Used Ventilation Systems in Hard Coal Mining
Authors: Grodzicka Aneta , Plewa Franciszek , Krause Marcin , Figiel Andrzej , Rozmus Magdalena
Published: January 2022

The way in which rescue actions are carried out in a hard coal mine is conditioned by a number of factors, including the type, scale, and location of the hazard; location of employees at the danger and level of their endangerment; and the ventilation system used in the impacted area. In this article, the importance and necessity to take into account a human factor, specifically the propensity for risky behavior, alongside the selection of rescuers for rescue action is pointed out. As an introduction to the key research studies presented in this article, main ventilation systems used in hard coal mines are described and three real cases of natural hazard occurrences in hard coal mines are discussed. An analysis of these events has shown that the degree of difficulty of a rescue action depends, among other aspects, on the ventilation system applied. Next, a study covering a synthetic assessment of 25 mining rescuers taking into account the ‘risky behavior’ parameter is presented. The results were interpreted considering the—described earlier—cases of hazard occurrence in coal mines and ventilation systems applied there. For the research sample, a selection of rescuers to carry out actions in particular types of ventilation systems, taking as a criterion the mark they obtained in the synthetic assessment, is proposed.
Measuring Technologies for CFB Solid Circulation Rate: A Review and Future Perspectives
Authors: Liu Xiandong , Zhang Man , Zhang Shuangming , Ding Yi , Huang Zhong , Zhou Tuo , Yang Hairui , Yue Guangxi
Published: January 2022

Solid circulation rate (Gs) represents the mass flux of circulating particles in circulating fluidized bed (CFB) systems and is a significant parameter for the design and operation of CFB reactors. Many measuring technologies for Gs have been proposed, though few of them can be applied in industrial units. This paper presents a comprehensive study on measuring technologies, and the results indicate that though the accumulation method is most widely applied, it is constrained by the disturbance of normal particle circulation. Some publications have proposed mathematic models based on pressure drop or other parameters to establish Gs measurement models; these necessitate the accurate modeling of complicated gas-solid flows in industrial devices. Methods based on certain measurement devices to specify parameters like velocity require device endurance in the industrial operation environment and stable local gas-solid flow. The Gs measuring technologies are strongly influenced by local gas-solid flow states, and the packed bed flow in standpipes make the bottom of standpipes an ideal place to realize Gs measurement.
The Impact of HVAC on the Development of Autonomous and Electric Vehicle Concepts
Authors: König Adrian , Mayer Sebastian , Nicoletti Lorenzo , Tumphart Stephan , Lienkamp Markus
Published: January 2022

Automation and electrification are changing vehicles and mobility. Whereas electrification is mainly changing the powertrain, automation enables the rethinking of the vehicle and its applications. The actual driving range is an important requirement for the design of automated and electric vehicles, especially if they are part of a fleet. To size the battery accordingly, not only the consumption of the powertrain has to be estimated, but also that of the auxiliary users. Heating Ventilation and Air Conditioning (HVAC) is one of the biggest auxiliary consumers. Thus, a variable HVAC model for vehicles with electric powertrain was developed to estimate the consumption depending on vehicle size and weather scenario. After integrating the model into a tool for autonomous and electric vehicle concept development, various vehicle concepts were simulated in different weather scenarios and driving cycles with the HVAC consumption considered for battery sizing. The results indicate that the battery must be resized significantly depending on the weather scenario to achieve the same driving ranges. Furthermore, the percentage of HVAC consumption is in some cases higher than that of the powertrain for urban driving cycles, due to lower average speeds. Thus, the HVAC and its energy demand should especially be considered in the development of autonomous and electric vehicles that are primarily used in cities.
Occurrence of Low-Level Jets over the Eastern U.S. Coastal Zone at Heights Relevant to Wind Energy
Authors: Aird Jeanie A. , Barthelmie Rebecca J. , Shepherd Tristan J. , Pryor Sara C.
Published: January 2022

Two years of high-resolution simulations conducted with the Weather Research and Forecasting (WRF) model are used to characterize the frequency, intensity and height of low-level jets (LLJ) over the U.S. Atlantic coastal zone. Meteorological conditions and the occurrence and characteristics of LLJs are described for (i) the centroids of thirteen of the sixteen active offshore wind energy lease areas off the U.S. east coast and (ii) along two transects extending east from the U.S. coastline across the northern lease areas (LA). Flow close to the nominal hub-height of wind turbines is predominantly northwesterly and southwesterly and exhibits pronounced seasonality, with highest wind speeds in November, and lowest wind speeds in June. LLJs diagnosed using vertical profiles of modeled wind speeds from approximately 20 to 530 m above sea level exhibit highest frequency in LA south of Massachusetts, where LLJs are identified in up to 12% of hours in June. LLJs are considerably less frequent further south along the U.S. east coast and outside of the summer season. LLJs frequently occur at heights that intersect the wind turbine rotor plane, and at wind speeds within typical wind turbine operating ranges. LLJs are most frequent, intense and have lowest core heights under strong horizontal temperature gradients and lower planetary boundary layer heights.
Selected Mathematical Models Describing Flow in Gas Pipelines
Authors: Osiadacz Andrzej J. , Gburzyńska Marta
Published: January 2022

The main aim of simulation programs is to study the behavior of gas pipe networks in certain conditions. Solving a specified set of differential equations describing transient (unsteady) flow in a gas pipeline for the adopted parameters of load and supply will help us find out the value of pressure or flow rate at selected points or along selected sections of the network. Transient gas flow may be described by a set of simple or partial differential equations classified as hyperbolic or parabolic. Derivation of the mathematical model of transient gas flow involves certain simplifications, of which one-dimensional flow is most important. It is very important to determine the conditions of pipeline/transmission network operation in which the hyperbolic model and the parabolic model, respectively, should be used. Parabolic models can be solved numerically in a much simpler way and can be used to design simulation programs which allow us to calculate the network of any structure and any number of non-pipe elements. In some conditions, however, they describe the changes occurring in the network less accurately than hyperbolic models do. The need for analysis, control, and optimization of gas flows in high-pressure gas pipelines with complex structure increases significantly. Very often, the time allowed for analysis and making operational decisions is limited. Therefore, efficient models of unsteady gas flows and high-speed algorithms are essential.
Two Birds with One Stone: Bioplastics and Food Waste Anaerobic Co-Digestion
Authors: Kakadellis Sarah , Lee Po-Heng , Harris Zoe M.
Published: January 2022

Following the BBC’s Blue Planet II nature documentary series on marine ecosystems, plastic packaging has come under public fire, with consumers demanding greener alternatives. The biodegradable properties of some bioplastics have offered a potential solution to the global challenge of plastic pollution, while enabling the capture of food waste through anaerobic digestion as a circular and energy-positive waste treatment strategy. However, despite their increasing popularity, currently bioplastics are being tested in environments that do not reflect real-life waste management scenarios. Bioplastics find their most useful, meaningful and environmentally-sound application in food packaging—why is there so little interest in addressing their anaerobic co-digestion with food waste? Here, we provide a set of recommendations to ensure future studies on bioplastic end-of-life are fit for purpose. This perspective makes the link between the environmental sustainability of bioplastics and the role of food waste anaerobic digestion as we move towards an integrated food–energy–water–waste nexus. It shines light on a novel outlook in the field of bioplastic waste management while uncovering the complexity of a successful path forward. Ultimately, this research strives to ensure that the promotion of bioplastics within a circular economy framework is supported across waste collection and treatment stages.
Impact of Respiratory Fluctuation on Hemodynamics in Human Cardiovascular System: A 0-1D Multiscale Model
Authors: Li Ruichen , Sughimoto Koichi , Zhang Xiancheng , Wang Sirui , Hiraki Yuto , Liu Hao
Published: January 2022

To explore hemodynamic interaction between the human respiratory system (RS) and cardiovascular system (CVS), here we propose an integrated computational model to predict the CVS hemodynamics with consideration of the respiratory fluctuation (RF). A submodule of the intrathoracic pressure (ITP) adjustment is developed and incorporated in a 0-1D multiscale hemodynamic model of the CVS specified for infant, adolescent, and adult individuals. The model is verified to enable reasonable estimation of the blood pressure waveforms accounting for the RF-induced pressure fluctuations in comparison with clinical data. The results show that the negative ITP caused by respiration increases the blood flow rates in superior and inferior vena cavae; the deep breathing improves the venous return in adolescents but has less influence on infants. It is found that a marked reduction in ITP under pathological conditions can excessively increase the flow rates in cavae independent of the individual ages, which may cause the hemodynamic instability and hence increase the risk of heart failure. Our results indicate that the present 0-1D multiscale CVS model incorporated with the RF effect is capable of providing a useful and effective tool to explore the physiological and pathological mechanisms in association with cardiopulmonary interactions and their clinical applications.
Ultrasonic Atomization: New Spray Characterization Approaches
Authors: Panão Miguel
Published: January 2022

In particle engineering, spray drying is an essential technique that depends on producing sprays, ideally made of equal-sized droplets. Ultrasonic sprays appear to be the best option to achieve it, and Faraday waves are the background mechanism of ultrasonic atomization. The characterization of sprays in this atomization strategy is commonly related to the relation between characteristic drop sizes and the capillary length produced by the forcing frequency of wavy patterns on thin liquid films. However, although this atomization approach is practical when the intended outcome is to produce sprays with droplets of the same size, drop sizes are diverse in real applications. Therefore, adequate characterization of drop size is paramount to establishing the relations between empirical approaches proposed in the literature and the outcome of ultrasonic atomization in actual operating conditions. In this sense, this work explores new approaches to spray characterization applied to ultrasonic sprays produced with different solvents. The first two introduced are the role of redundancy in drop size measurements to avoid resolution limitation in the measurement technique and compare using regular versus variable bin widths when building the histograms of drop size. Another spray characterization tool is the Drop Size Diversity to understand the limitations of characterizing ultrasonic sprays solely based on representative diameters or moments of drop size distributions. The results of ultrasonic spray characterization obtained emphasize: the lack of universality in the relation between a characteristic diameter and the capillary length associated with Faraday waves; the variability on drop size induced by both liquid properties and flow rate on the atomization outcome, namely, lower capillary lengths produce smaller droplets but less efficiently; the higher sensibility of the polydispersion and heterogeneity degrees in Drop Size Diversity when using variable bin widths to build the histograms of drop size; the higher drop size diversity for lower flow rates expressed by the presence of multiple clusters of droplets with similar characteristics leading to multimodal drop size distributions; and the gamma and log-normal mathematical probability functions are the ones that best describe the organization of drop size data in ultrasonic sprays.
Influence of Partial Replacements of NaCl by KCl on Quality Characteristics and the Heterocyclic Aromatic Amine Contents of Bacon
Authors: Du Hongzhen , Li Xiangao , Wang Qiang , Liu Qian , Chen Qian , Kong Baohua
Published: January 2022

The influence of partial replacements of NaCl by KCl (0, 10, 20, and 30%) on the heterocyclic aromatic amine (HAAs) contents and quality characteristics of bacon were investigated. The Na+ content, moisture, aw, pH, L* value, and sensory saltiness decreased and K+ content, a* value, and sensory bitterness increased significantly with increased substituting rates of NaCl by KCl (p < 0.05). There were no significant differences between the control and KCl substitution samples for the b* value, redness, and sensory off-odor (p > 0.05). The creatine content was not affected by the different KCl-substituting rates during the marinating process (p > 0.05), but it diminished in the smoking and frying processes (p < 0.05). The increase in the KCl-substituting rates increased the total heterocyclic aromatic amine (HAA) contents in fried bacon (p < 0.05). Moreover, the nonpolar HAA content in bacon was higher than the polar HAA content (p < 0.05). In summary, the partial replacement of NaCl by KCl increased the total HAA content and led to changes in bacon quality.
Retail Packaging Affects Colour, Water Holding Capacity, Texture and Oxidation of Sheep Meat more than Breed and Finishing Feed
Authors: Ha Minh , Warner Robyn Dorothy , King Caitlin , Wu Sida , Ponnampalam Eric N.
Published: January 2022

This study investigated the CIELab colour, water holding capacity, texture and oxidative stability of sheep meat from different breeds, finishing feeds, and retail packaging methods. Leg primal cuts from a subset of Composite wether lambs (n = 21) and Merino wether yearlings (n = 21) finished on a standard diet containing grain and cereal hay, a standard diet with camelina forage, or a standard diet with camelina meal, were used in this study. Semimembranosus and Vastus lateralis were packaged in vacuum skin packaging (VSP), or modified atmosphere packaging with 80% O2 and 20% CO2 (HioxMAP), or with 50% O2, 30% N2, and 20% CO2 (TrigasMAP). Packaging had a greater effect (p < 0.001) on L*, a*, b*, hue, and chroma than the effects from breed and finishing feed. Purge loss was affected by packaging. Cooking loss was affected by breed for Semimembranosus and packaging for both muscle types. HioxMAP and TrigasMAP increased WBSF and Texture Profile Analysis hardness of the meat compared to VSP. Lipid oxidation, assessed by TBARS, were lower in camelina forage or camelina meal supplemented diets and TrigasMAP compared to standard diet and HioxMAP, respectively. Total carbonyl and free thiol content were lower in VSP. Thus, supplementing feed with camelina forage or meal and lowering oxygen content in retail packaging by TrigasMAP or VSP are recommended to ensure optimal sheep meat quality.
RP-HPLC-ESI-QTOF-MS Qualitative Profiling, Antioxidant, Anti-Enzymatic, Anti-Inflammatory, and Non-Cytotoxic Properties of Ephedra alata Monjauzeana
Authors: Khattabi Latifa , Boudiar Tarek , Bouhenna Mustapha Mounir , Chettoum Aziez , Chebrouk Farid , Chader Henni , Lozano-Sánchez Jesús , Segura-Carretero Antonio , Nieto Gema , Akkal Salah
Published: January 2022

An investigation was conducted to study the beneficial effects of Ephedra alata monjauzeana crude extract (EamCE). The chemical profile was determined using RP-HPLC–ESI-QTOF-MS analysis, revealing the presence of twenty-one flavonoids and phenolic acids. A series of antioxidant assays was carried out using ten different methods. The EamCE has demonstrated a significant antioxidant potential, with interesting IC50 values not exceeding 40 µg/mL in almost activities. Likewise, a significant inhibition of key enzymes, involved in some health issues, such as Alzheimer’s disease, diabetes, hyperpigmentation, dermatological disorders, gastric/urinary bacterial infections, and obesity, was observed for the first time. The IC50 values ranged from 22.46 to 54.93. The anti-inflammatory and non-cytotoxic activities were assessed by heat-induced hemolysis and cell culture methods, respectively; the EamCE has shown a prominent effect in both tests, notably for the anti-inflammatory effect that was superior to the reference compound “diclofenac” (IC50: 71.03 ± 1.38 > 70.23 ± 0.99 (µg/mL)). According to these results, this plant could be used in a large spectrum as a food supplement, as a natural remedy for various physiological disorders and pathologies; and it might serve as a preventive and health care agent.