bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of diagnostic and triage accuracy and usability of a symptom checker in an emergency department: observational study (Preprint)
Authors: Fraser Hamish Scott , Cohan Gregory , Koehler Christopher , Anderson Jared , Lawrence Alexis , Pateña John , Bacher Ian , Ranney Megan
Published: April 2022

BACKGROUND

Symptom checkers (SCs) are diagnostic decision support apps for patients, used by tens of millions of people annually. They are designed to provide diagnosis and triage advice and assist users in seeking the appropriate level of care. Little evidence is available on their diagnostic and triage accuracy with direct use by patients for urgent conditions.

OBJECTIVE

To determine the diagnostic and triage accuracy and usability of a symptom checker in use by for patients presenting to an emergency department

METHODS

We recruited a convenience sample of English-speaking patients presenting for care in an urban emergency department. Each consenting patient used a leading SC from Ada Health prior to ED evaluation. Diagnostic accuracy was evaluated, comparing (1) the SC’s diagnoses and (2) those of 3 independent emergency physicians viewing the patient-entered symptom data, to (3) the final diagnoses from the ED evaluation. The Ada diagnoses and triage were also critiqued by the independent physicians. Patients completed a usability survey based on the Technology Acceptance Model.

RESULTS

Forty participants (80% of those approached, mean age 40.1 years (range 18 – 76); 65% female, 68% White, 48% Hispanic or Latino, completed the SC assessment and usability survey. Sensitivity for at least one of the ED final diagnoses by Ada (based on it’s top 5 diagnoses) was 70.0% (95% CI 54% – 86%) close to the mean sensitivity for the 3 physicians (on their top 3 diagnoses) of 68.9%. The physicians rated Ada triage decisions as 62% fully agree, and 24% safe but too cautious. It was rated unsafe and too risky in 22% by at least one physician, 14% by two and 5% by all 3, although none of the under-triaged patients suffered an adverse event in the ED. Some cases only had symptoms recorded for their ED diagnosis, limiting analysis. Usability was rated highly, participants agreed or strongly agreed a mean of 84.6% with the 7 TAM usability questions, although “satisfaction” and “enjoyment” were rated low.

CONCLUSIONS

This study provides preliminary evidence that a SC can provide acceptable usability and diagnostic accuracy for patients with a variety of urgent conditions. 14% of SC triage recommendations were deemed unsafe and too risky by at least 2 physicians based on the symptoms recorded. Larger studies are needed of diagnosis and triage performance with direct patient use in different clinical environments.
Evaluation of a Digital Self-management Platform for Patients With Chronic Illness in Primary Care: Qualitative Study of Stakeholders’ Perspectives (Preprint)
Authors: van de Vijver Steven , Hummel Deirdre , van Dijk Annericht Hester , Cox Jan , van Dijk Oscar , Van den Broek Nicoline , Metting Esther
Published: April 2022

BACKGROUND

Population aging and multimorbidity has led to increasing chronic care needs associated with new challenges in managing growing costs, rising health care professional workloads, and the adoption of rigorous guidelines. These issues could all benefit from greater digitalization and a more patient-centered approach to chronic care, a situation brought to the fore by the COVID-19 pandemic. Little is known about real-life use in primary care.

OBJECTIVE

This study aimed to explore the views, thoughts, usability, and experiences concerning a recently introduced digital self-care platform for chronic conditions in 3 Dutch primary care practices.

METHODS

We conducted an explorative study combining questionnaires and interviews among patients and general practitioners from 3 general practices that used the digital platform. Questionnaires were sent to patients in each practice to seek the views and experiences of both patient nonusers (n=20) and patient users (n=58) of the platform, together with standardized questionnaires about illness perception and quality of life. In addition, patients (n=15) and general practitioners (n=4) who used the platform took part in semistructured interviews. We transcribed interviews verbatim and performed qualitative content analysis using a deductive approach. The results of the questionnaires were analyzed with descriptive analysis.

RESULTS

Among patients who had not actively used the platform but had received an explanation, only 35% (7/20) would recommend its use due to concerns over communication and handling. However, this percentage increased to 76.3% (45/59) among the people who actively used the platform. Interviews with patients and general practitioners who used the platform uncovered several key benefits, including reduced time requirements, reduced workload, improved care quality, and improved accessibility due to the greater patient-centeredness and use of different communication tools. In addition, the self-management tool led to greater patient autonomy and empowerment. Although users considered the platform feasible, usable, and easy to use, some technical issues remained and some patients expressed concerns about the reduction in human contact and feedback.

CONCLUSIONS

The overall experience and usability of the platform was good. Support for the online self-management platform for chronic care increased when patients actively used the tool and could experience or identify important advantages. However, patients still noted several areas for improvement that need to be tackled in future iterations. To ensure benefit in the wider population, we must also evaluate this platform in cohorts with lower digital and health literacy.
Issue or Intervention in Demographic/Disease/Condition: Method/Study Design (Preprint)
Authors: Account Developer
Published: April 2022

UNSTRUCTURED

Issue or Intervention in Demographic/Disease/Condition: Method/Study Design Issue or Intervention in Demographic/Disease/Condition: Method/Study Design Issue or Intervention in Demographic/Disease/Condition: Method/Study Design Issue or Intervention in Demographic/Disease/Condition: Method/Study Design
Exploring users' health behavior changes in online health communities: Heuristic-Systematic Perspective Study (Preprint)
Authors: Gong Liyue , Guo Yi , Jiang Hao , Kong Yi , Wu XuSheng , Gao YunYun , Hu DeHua
Published: April 2022

BACKGROUND

With the continuous advancement of internet technology, the internet plus medical service provides a new solution to solve the shortage of medical resources and uneven distribution. Online health communities (OHCs) that emerged at the historical moment have flourished with their advantages, such as being free from location and time constraints. Understanding the users' behavior change via engagement in OHCs is necessary to support the development of internet medicine and promote public health.

OBJECTIVE

Drawing on the heuristic-systematic model, we explored the impact of users' engagement and information processing in OHCs on their healthy behavior changes.

METHODS

The hypotheses of our research model were developed based on the PADM and HSM. A questionnaire was developed through previous studies and verified using a presurvey. We used structural equation modeling to test the research hypotheses.

RESULTS

The perceived benefits positively affect information needs, thereby promoting users' engagement in OHCs; engagement in OHCs has a significant positive impact on health behavior change, and it also significantly positively affects users' health behavior change through systematic processing indirectly.

CONCLUSIONS

Our study offers support for the usefulness of the PADM and HSM in explaining users' health behavior changes. For practitioners, this study introduces influence processes as policy tools that managers can employ for health-promoting with mHealth.
Kecenderungan Arah Penelitian Pada Tugas Akhir Mahasiswa Fkip Universitas Bung Hatta
Authors: Har Erman
Published: April 2022

Penelitian ini mempelajari kecenderungan arah penelitian yang dilaksanakan oleh mahasiswa fkip  Universitas Bung Hatta pada pelaksanaan tugas akhir. Sasarannya 10 orang mahasiswa dari  tujuh program studi dengan jumlah responden sebanyak 70 orang. Analisis data secara deskriptif, t-test . Hasil penelitian menunjukkan bahwa arah penelitian yang dilakukan oleh mahasiswa pada tugas akhir memperlihatkan kecenderungan mengarah pada penelitian eksperimen, diikuti dengan penelitian survei, dan penelitian pengembangan. Uji t-test menunjukkan bahwa terdapat perbedaan yang signifikan  antara penelitian yang dilakukan oleh mahasiswa laki-laki dengan mahasiswa perempuan terutama arah penelitian eksperimen lebih tinggi dilakukan oleh mahasiswa perempuan di banding dengan mahasiswa laki-laki. Implikasi penelitian ini adalah mahasiswa lebih memahami penelitian eksperimen dibanding dengan penelitian yang lain. Oleh sebab itu para dosen yang mengajar metode penelitian pada berbagai program studi dapat memberikan materi yang seimbang untuk berbagai jenis penelitian.
INDUSTRY AND RESEARCH COLLABORATION A MODEL FOR INDIA
Authors: GOEL ROSHANI
Published: April 2022

The purpose of this paper is to understand the technological innovation taken place in pharmaceutical industry based on institutional research and the gaps between the investment made in research the utilisation of research by the industry. Though the topic is open for argument but it will be beneficial for public policy makers concerned with science and technology and economist thriving for technological change and innovation. The paper aims at putting a discussion for a need on investment and private businesses collaboration with institutional research. This gives space and time for new knowledge to transform the existing technology for betterment of life. It critically talks in the context of pharmaceutical companies in India while getting an outline from industrially developed countries. It highlights the fact that first industry academia collaboration enhancement agenda was held on 5th March 2013, in New Delhi. This itself explains the requirement of institutional research for technological upliftment which will further be supported by literature presented by the researchers.

The international scenario showed that 1980’s Bayh Dole Act of United States is the best model to strengthen the industry and research collaboration. The model has been implemented worldwide. This paper presents the analysis of countries like U.S.A, United Kingdom, China and OCED countries. The reason for this is that these countries have well developed academia industry collaboration and strategic partnership between them. Though these countries have better GDP and stable economy. However, India is a growing economy and new ideas will help it in making it stronger.

India is at a starting stage of developing the process academia research and industry collaboration, so there is a way for new strategies to make way in the bottleneck for collaboration. Even in India Bayh Dole Act has been implemented under different name. But one of the major challenges regarding its implementation that India receives is in transparency and clarity in terms of policy and lack of inputs from different organisations. In addition, different are positive about India’s growth and in order to sustain such growth in coming years it is imperative to have technological advancement and innovation to accelerate to propel the economic growth of the country. Thus, research centres near firms seems to get more related to research work concerned or required by the industry. In other words, in industry research collaboration geographical proximity has main of the main role to play in it. The study bases its argument on rich literature that advocates that the collaboration is beneficial for both research and industry.
The Impact of Talking Classroom in Teaching and Learning 3Rs in Public Pre-Primary Schools in Ilemela Municipality, Mwanza-Tanzania
Authors: Mugyabuso Witness Berthesa , Tangi Felista
Published: April 2022

The study confined to public pre-primary schools based on assumption that early age is the best opportunity to teach children the skills of reading, writing and arithmetic (3Rs). The study aim is to assess the impact of talking classroom in teaching and learning basic 3Rs skills in public pre-primary schools in Ilemela Municipality, Mwanza, Tanzania. The study conducted in ten public primary schools which involved twenty participants whereby ten was pre-primary teachers and ten was public primary school head teachers. The study employed qualitative approach method with a case study design. In sampling technique, the study adopted convenience and purposeful sampling technique, which included critical case to obtain participants. Data collected through structured interviews, observation checklist and documentary review guideline. The study revealed that pre-primary teachers in public primary schools had positive perceptions on the use of talking classroom in teaching and learning 3Rs in pre-primary children. Also, the study revealed that for effective use of 3Rs in public pre-primary classroom, there must be enough teaching and learning materials. The study recommends that the government should ensure the increase of school capitation funds especially in primary schools.
ВЛИЯНИЕ ПОВЕРХНОСТНОГО ЗАРЯДА ЭЛЕКТРЕТОВ НА ГРИБОСТОЙКОСТЬ ФТОРУГЛЕРОДНЫХ ПОЛИМЕРНЫХ МАТЕРИАЛОВ
Authors: Елинсон В.М. , Аболенцев А.С. , Ходырев Т.В. , Щур П.А.
Published: April 2022

В данной работе рассматриваются результаты исследования грибостойкости наноструктурированных фторуглеродных пленок, созданных на поверхности полимеров, в зависимости от величины поверхностного заряда. По-видимому, на грибостойкость пленки влияет не только поверхностный заряд, но и другие характеристики поверхности, такие как рельеф и химический состав. Фторуглеродные пленки были сформированы в условиях переходных процессов с использованием двухкомпонентной фторуглеродной газовой смеси (CF4 + С6Н12).
СИЛА СКАНИРУЮЩЕГО СИЛОВОГО МИКРОСКОПА
Authors: Синицына О.В. , Яминский И.В.
Published: April 2022

У атомно-силового микроскопа есть и другое название – сканирующий силовой микроскоп. При получении изображений с его помощью необходимо использовать обратную связь для контроля силы взаимодействия между зондом и образцом. Как правило, ее нужно поддерживать на минимальном значении, чтобы не деформировать образец и зонд в процессе измерений. Напротив, в нанолитографии и наноиндентировании следует прилагать большую силу, чтобы получить четкий рисунок на поверхности. В данной статье рассматривается, какие силы возникают и сколько энергии затрачивается при возникновении типичного дефекта в виде шестиконечной звезды на графите.
Personalized Mobile App Recommendation by Learning User’s Interest from Social Media
Authors: Sawant Miss. Pratiksha Yuvraj , Salunke Mr. Mangesh D
Published: April 2022

Abstract: In social media, user interests and knowledge are vital but often overlooked resources. There are a few ways to get a sense of what people are known for, such as Twitter lists and LinkedIn Skill Tags, but most people are untagged, so their interests and expertise are effectively hidden from applications like personalised recommendation and community detection and expert mining. We obtain personalised app recommendations by learning the interest's association between applications and tweets by introducing an unique generative model called IMCF+ to convert user interest from rich tweet information to sparse app usage. We analyse the performance of this technique predicts the top ten apps with an 82.5 percent success rate using only 10% training data. Furthermore, in the high sparsity situation and user cold-start scenario, this purpose technique outperforms the other six state-of-the-art algorithms by 4.7 percent and 10%, demonstrating the effectiveness of our technology. All of these findings show that our method can reliably extract user interests from tweets in order to aid in the solution of the personalised app recommendation problem. Keywords: Social Media, User Profile, deep learning, Privacy, matrix factorization,App recommendation.
Microscopic Simulation-Based Model analysis at Jaipur
Authors: Dwivedi Avinash , Ram Ramoo , Pareek Ravi Kant
Published: April 2022

Abstract: Socio-Economic development of a country includes sustainable transportation system. As simulation is safer, less expensive and faster than field implementation and testing, Microscopic simulation models have been widely used in transportation operations and management analysis. The accuracy and reliability decide the usefulness of these models in making design and traffic control. Nowin India, VISSIM which is microscopic traffic simulation software is used. However, modifications to the default behavioral parameters are essential to effectively simulate Indian heterogeneous traffic conditions. In this study, the queue length and travel time of link connected to Narayan Singh Circle and Trimurti Circle intersection is analyzed before and after transfer the bus stop at Narayan Singh Circle to the periphery of city. For this, intersections are designed in VISSIM software and traffic flow performance is validated via VISSIM. Then average vehicular delay under different traffic volumes was analyzed. The results are finally compared before and after transfer the bus stop at Narayan Singh Circle to the peripheryof city. Keywords: Non-destructive Testing, Concrete, Structural Health Monitoring, Ultrasonic Pulse Velocity,Rebound Hammer.
Foot Operated Washing Machine
Authors: Mandavkar Omkar A. , Komawar Tejas P. , Gawad Dipesh R. , Gupta Shubham V. , Bari Shaikh Abdul
Published: April 2022

Abstract: The foot operated washing machine is a huge innovation all by itself. Foot operated washing machine is especially designed for its use for washing laundry by means of foot application. Today, because of non-renewable energy cries out its basic need to use energy in another way or to save energy. This project involves the construction and use of the foot operated washing machine. The next pages in the paper include the constructions of foot operated washing machine, its raw material, its operation , benefits of the foot washing machine in terms of the actual electronic washing machine save time, water, electricity and not very expensive. His main expectation is exercises with the application of the foot to wash the cloths. Keywords: foot operated, pedal, chain, cloths, washing, rinsing
Transformation of perception and requirements for seaside towns, their saturation, architectural appearance
Authors: Ilicheva Daria A.
Published: April 2022

Introduction. The present-day life of Russian seaside towns, their architecture and aesthetics feature an urgent demand for improving the quality and adding new choice options for residents and guests, the variability in the functional saturation of architectural objects and, what is also extremely important, the holistic and comfortable appearance of buildings.

Materials and methods. The author will consider how the perception of life in seaside towns has changed, what requests have appeared, and what can be done to meet them. These tasks will be considered in a social context from the standpoint of each category of residents. The author uses Sevastopol as a case city.

Results. The author has identified rapidly changing workflow organization methods, tightening standards of comfortable places of residence, their surroundings, and tougher requirements for an urban environment as a whole. Artistic and stylistic unity, environmental aesthetics have become obligatory and integral constituents of comfortable living in a space capable of giving a quick response to the needs of a resident. It is necessary to trace the historical and modern trends inherent in a particular place, to understand what requests citizens have, what is needed for the harmonious development and life in it, what special features should be inherent in new residential buildings, what artistic and stylistic proposals are to be made.

Conclusions. A set of common problems and requests, that require solutions and entail a number of circumstances creating discomfort in urban living, has been formulated for each category of citizens identified using cultural and economic criteria. To solve these problems, the authors suggests concepts of a development model, demonstrating multifunctionality and variability, creating a holistic comfortable environment, focusing both on socioeconomic issues and equally important aspects of architectural and stylistic perception. Recommendations on the “use” of this model and the development of urban appearance are also made.
Modeling thermal and wind processes in the near-wall layer of building envelopes subjected to insolation
Authors: Giyazov Adham I. , Mirzoev Saidmukhammad M. , Abdulrahman Karum
Published: April 2022

Introduction. Insolation patterns play a decisive role in creating a comfortable microclimatic environment in building spaces. This is a relevant problem of thermal safety assurance for modern construction projects and better sanitary and hygienic conditions of indoor environments. The radiant energy of the sun is a priority in the architectural and structural design and the design of thermal shells of buildings.

Materials and methods. Theoretical, full-scale microclimatic and thermophysical field studies on structural envelopes of buildings were conducted to identify the contribution of insolation to thermal and wind patterns in the near-wall layer of building envelopes. These methods were verified using the analysis of research techniques, developed by domestic and foreign authors specializing in thermal physics of energy-efficient buildings. The authors developed a set of methods allowing to conduct new experimental studies of physical processes in the thermal air shell of the near-wall layer of envelope structures of real buildings.

Results. As a result of the field studies of thermal and wind processes underway near the structural envelope of buildings subjected to prolonged insolation, the authors learned that the microclimate turns uncongenial in the near-wall air layer of buildings and in building spaces. This microclimate has unique thermal conditions. The extent of impact, made by the external walls of buildings on the generation and regulation of thermal and wind patterns of the near-wall microclimate was identified. A model of convective flows of the near-wall microclimatic layer of multi-storied residential buildings was developed. These convective flows demonstrate high capacity and vertical mobility and cause the air mass to travel through the space of a building floor that has columns due to the thermal contrast between the opposite walls of buildings.

Conclusions. The authors have identified the contribution of solar energy to thermal and wind conditions in the air layer of thermal protective shells of buildings subjected to insolation. The mechanism, underlying the thermophysical processes arising in the course of interaction between insolation and the active surface of the thermal protective shell, is identified. The authors have formulated the preconditions for adjusting the design air temperature when assessing the thermal stability of building envelopes, taking into account the insolation conditions of facade systems of buildings. The preconditions for the relationship between outdoor and indoor air environments, as well as methods of natural aeration of premises have been demonstrated.
The concept for developing a programme model for the scientific and technical support of lifecycles of unique deeply embedded buildings
Authors: Lapidus Azariy A. , Topchiy Dmitry V. , Shevchenko Irina S.
Published: April 2022

Introduction. Scientific and technical support (STS) serves as a quality control tool and a safety assurance instrument employed in the process of construction of unique buildings and structures having higher levels of importance. However, along with the lack of approved provisions on performance of these types of work at the stages of engineering surveys, design and construction, no scope of work is specified for deeply embedded buildings and structures. The aim of the study is to develop a model of a programme that will ensure the automated selection of works on the basis of pre-set parameters for of unique deeply embedded buildings and structures.

Materials and methods. The authors propose a step-by-step development pattern for an STS support programme model for unique deeply embedded buildings and structures. A step-by-step development of the model of a program for scientific and technical support of unique buildings and structures with the large deepening is proposed, which is based on: a comprehensive analysis of modern regulatory documents and existing ideas for improving the process of scientific and technical support, which forms a database of works of STS; general and special parameters for selecting works from a database of works have been established, which correspond with the under consideration type of unique object; the need for these parameters is reflected in the development of the model of a program and further use. The authors have proven the need to use these parameters in the process of developing the programme model.

Results. The programme model of STS support for unique deeply embedded buildings and structures tracks the actions taken by its users, the programme operation, the use of parameters by the programme, as well as internal interactions between the programme and the database.

Conclusions. The presented stages of developing a programme model will not only computerize the selection of works on the basis of general and special parameters within the framework of STS, they will also improve the STS support in general.
Efecto de la edad al primer parto sobre la longevidad, el número de días en producción y la producción de leche durante la vida productiva de las vacas lecheras Holstein y Pardo Suizo en Honduras
Authors: Copas Medina Karen Alessa , Valladares Rodas Manuel , Baeza Rodríguez Juan José , Magaña Monforte Juan Gabriel , Segura Correa José Candelario
Published: April 2022

The aim was to determine the effect of age at first calving on longevity, lifetime number of productive days and lifetime milk yield of Holstein and Brown Swiss cows in the tropical savanna of Honduras.  The information was collected from three dairy farms with Holstein (n = 1,391) and four farms with Brown Swiss cows (n = 480), born from 1993 to 2013, managed under intensive systems. The statistical model that described the variables of interest included the effect of farm, group of age at first calving, period of birth, season of birth and the interaction farm x period and the residual error. Effects of farm, period group of age at first calving and interaction of farm x period were found on the response variables. A favorable effect of the cows that had their first calving at an earlier age on lifetime number of days in production and lifetime milk yield, and an increase in longevity, in both Holstein and Brown Swiss cows (P<0.05). In conclusion, cows that calved at an early age had more productive days in the farm and produced more milk during their productive life. Therefore, make the heifers to calf at an early age could be a management strategy to increase productivity in the farm. However, the weight at first calving and physiological aspects of the animal should be taken into consideration.
Efecto del aceite de orégano en las propiedades fisicoquímicas, texturales y sensoriales del queso panela
Authors: Sánchez-Zamora Niriel , Cepeda-Rizo Mónica Dinorah , Tamez-Garza Katty Lorena , Rodríguez-Romero Beatriz Adriana , Sinagawa-García Sugey Ramona , Luna Maldonado Alejandro Isabel , Flores-Girón Emmanuel , Méndez-Zamora Gerardo
Published: April 2022

Los aceites esenciales obtenidos de plantas están siendo usados en la industria alimentaria debido a su efectividad antimicrobiana, antioxidante y sensorial. En esta investigación se evaluó el efecto del aceite esencial de orégano (AEO) en la elaboración del queso panela (QP) sobre sus propiedades fisicoquímicas, texturales y sensoriales en 15 días. Tres tratamientos se establecieron: testigo (QP1), AEO en 0.05 g/L (QP2) y 0.10 g/L de leche (QP3). El pH de los quesos fue mayor (P<0.05) al día 1 y menor al día 15, y la acidez menor en QP2 al día 1 y en QP3 al día 4. La luminosidad al día 1 fue mayor (P<0.05) en QP2 y menor en QP1. La dureza aumentó (P<0.05) en QP1 y disminuyó en QP2 al día 1 y 8. El pH, acidez y textura del queso se mantienen con 0.05 g AEO/L, y con 0.1 g AEO/L leche mejora la aceptación sensorial.
Incubación, pre-lisis y post-purificación en el rendimiento y pureza de ácidos nucleicos extraídos de sangre de cabras domésticas contenida en tarjetas FTA
Authors: Sancho-Blanco Carolina , Jiménez-Alfaro Esteban J. , Molina-Bravo Ramón , Umaña-Castro Rodolfo
Published: April 2022

Técnicas moleculares requieren extracciones de ácidos nucleicos en cantidad y pureza adecuadas. Este trabajo describe un modelo lineal generalizado (GLM) de un factor ajustado con efectos fijos sobre el rendimiento de ácido nucleico (ng/μl) y la pureza (A260/A280 y A260/A230), para cinco métodos de extracción de ADN utilizando tarjetas FTA con sangre de cabra (Capra aegagrus hircus). Se ensayaron dos métodos comerciales basados en columnas de sílice (Invitrogen y Macherey Nagel; MN), método resina quelante (Chelex), método CTAB y el método de fenol-cloroformo-alcohol isoamílico (PCI). Adicionalmente, para MN, se evaluó una etapa de incubación con tampón PBS (Phosphate Buffered Saline) en alta temperatura previa a la lisis y una etapa de purificación posterior a la extracción utilizando un modelo de efecto fijo de dos factores con interacción. Las concentraciones de ADN y las proporciones de pureza fueron variables; la concentración más alta se obtuvo con el kit MN (170.45 ng/μl), pero con deficiencias en la pureza (0.32 de A260/A230, 0.34 de A260/A280). A pesar de esto, todos los métodos de extracción generaron productos PCR con cebadores específicos D-loop (ADNmt). El efecto combinado de las etapas de pre-incubación y post-purificación arrojó valores de pureza satisfactorios (1.89 para A260/A230 y 1.65 para A260/A280), así como relaciones de concentración (476.78 ng/μl) con baja variabilidad. En conclusión, la concentración y pureza del ADN de muestras de sangre mejora considerablemente cuando se usa un kit comercial en combinación con incubación previa a la lisis y purificación posterior a la extracción. Estos ácidos nucleicos se sugieren para uso en potenciales aplicaciones moleculares a posteriori.
Efecto del comportamiento higiénico sobre la resistencia a la cría calcárea (Ascosphaera apis) en colonias de abejas africanizadas (Apis mellifera)
Authors: Medina-Flores Carlos Aurelio , Medina Medina Luis Abdelmir , Guzmán-Novoa Ernesto
Published: April 2022

El objetivo fue evaluar el comportamiento higiénico (CH) de abejas melíferas (Apis mellifera) africanizadas y su impacto en la resistencia a la ascosferosis causada por Ascosphaera apis. Se evaluó el CH y la población de abejas adultas y de cría de 50 colonias. Además, colonias con alto (>95 %) y bajo (<50 %) CH fueron inoculadas con A. apis y en ellas se determinó el número de crías con signos de ascosferosis (momias) durante 17 días, datos que se correlacionaron con su grado de CH. También se evaluó la susceptibilidad a A. apis de larvas de colonias con alto y bajo CH en un medio ambiente común, para separar efectos ambientales de genotípicos. El grado de CH entre colonias varió significativamente (CV>36 %), con 20 % de las colonias mostrando alto CH (>95 %) y éste no se correlacionó con la población de abejas adultas y cría. Las colonias con alto CH tuvieron un número significativamente menor de momias que las colonias con bajo CH y hubo una correlación negativa entre el CH y el número de momias (r= -0.63, P= 0.02). Además, las larvas de colonias con alto o bajo CH fueron igualmente susceptibles al hongo. Estos resultados sugieren que el CH y la susceptibilidad de las larvas no están asociados y que el principal mecanismo de protección contra A. apis en poblaciones de abejas africanizadas es el CH. Por ello, la selección de colonias con alto comportamiento higiénico podría contribuir a mejorar la salud y productividad de las abejas melíferas.
Efecto de la viscosidad en el medio para la criopreservación espermática de gallo (Gallus gallus)
Authors: Herrera Barragán José Antonio , Huitrón José Manuel , Pérez-Rivero Juan José , Guzmán Sánchez Adrián , Ávalos Rodríguez Alejandro , Rosales Torres Ana María , Camarillo Flores Ricardo
Published: April 2022

En semen de mamíferos, se ha demostrado que la viscosidad influye de manera negativa para su conservación. En semen de aves los estudios sobre las características físicas de los eyaculados son limitados, particularmente la viscosidad no ha sido estudiada. Los medios para la criopreservación, no consideran la viscosidad para mantener la viabilidad espermática. Por lo cual, el objetivo de este estudio fue evaluar el efecto de la viscosidad en el medio para mantener su viabilidad post descongelación. Se determinó los parámetros de evaluación básica, de maduración y reacción acrosomal evaluando la presencia y distribución de Ca2+ mediante la co-incubación con clortetraciclina. Se realizaron 25 evaluaciones de pool seminal, criopreservados en medio Lake suplementado con 6 % de dimetilacetamida y con 0 % (Testigo), 10 %, 30 % y 45 % de ficoll, para ajustar la viscosidad del medio a condiciones similares a las del semen y del líquido oviductal y a un grado mayor de viscosidad. La movilidad espermática fue menor (P£0.05) en alícuotas con mayor porcentaje de ficoll. El porcentaje de espermatozoides vivos fue similar (P>0.05) en el testigo y todas las alícuotas con diferente porcentaje de ficoll. La maduración espermática presentó un mayor (P£0,05) porcentaje de espermatozoides no capacitados cuando se utilizó 10% de ficoll. De manera inversa el porcentaje de espermatozoides con reacción acrosomal fue menor (P£0,05) también cuando se utilizó 10 % de ficoll. Los resultados de este estudio demuestran que variaciones en el grado de viscosidad del medio, pueden mantener o incrementar la viabilidad espermática post descongelación.
Impacto económico y productivo de una mezcla herbal con derivados de colina en la producción de conejos
Authors: Jaurez-Espinosa Minerva , Hernández-García Pedro Abel , Osorio-Terán Amada Isabel , Mendoza-Martínez Germán David , Ojeda-Carrasco Juan José , Tapia-Rodríguez María Zamira , Espinosa-Ayala Enrique
Published: April 2022

El uso, así como el efecto productivo y económico de compuestos herbales en producción cunícola ha sido poco estudiado, por tal motivo el objetivo fue evaluar el efecto de una mezcla poliherbal rica en conjugados de colina a base de Trachyspermum ammi, Achyranthes aspera, Azadirachta indica y Citrullus colocynthis, sobre la respuesta económica y productiva de conejos para carne. Para lo cual se utilizaron 40 conejos Nueva Zelanda X California (30 días de edad), los cuales fueron aleatorizados en cinco grupos (0.0, 200, 400, 600 y 800 mg kg-1 de MS poliherbal, BioCholina®), el experimento tuvo una duración de 34 días. Se evaluó la respuesta productiva, calidad de la carne e indicadores económicos. Se utilizó un diseño completamente al azar con un arreglo de polinomios ortogonales para determinar efectos lineales y cuadráticos, con un nivel de significancia de P<0.05. Los resultados en cuanto a los parámetros productivos fueron similares entre tratamientos, excepto consumo de alimento (P=0.006) y conversión alimenticia (P=0.005) con un efecto linealmente a mayor concentración del poliherbal. La inclusión del poliherbal incrementó pH en carne (lineal, P=0.004) y coordenada b* (lineal, P=0.009), se observó que el tratamiento con 200 mg presentó los mejores indicadores económicos mejorando en 9 unidades porcentuales la razón ingresos-egresos. Se concluye que la adición de mezclas poliherbales a base de conjugado de colina natural no mejoró las variables productivas, sin embargo, se marca una tendencia económica favorable con la adición de 200 mg kg-1 de MS.
Axel Honneth’s Dialogue on Hegel’s Philosophy of Law
Authors: Euler Werner
Published: April 2022

This article has two purposes. First, it aims to present a detailed analysis of the argument of “recognition” or even of the “fight for recognition”, which Hegel uses in his fragments from Jena treating of the system of philosophy, especially of the philosophy of spirit. It will be necessary to determine precedently by means of an exact interpretation the content of that expressions, in order to criticize and to compare, his original significance in Hegel with the theoretical application made by Honneth. The special aim hereby is to correct and to explain the function of the “fight for recognition”, even in its sharpening significance as a “fight for life and death”, with relation to the genesis of the state of right. Second, the figure of “recognition” is related to Hegel’s Philosophy of right in the version of 1820. In this work, recognition participates in the theory of “institutions” which is analyzed by Honneth as well. Apart from the merits gained through the endeavour to introduce a classic philosophical theory into a conception of justice in society, we feel obliged to state an error in Honneth‘s interpretation in so far as he understands an institution as a simple social community of communication (or as intersubjective community). For that reason, a conclusion based on a critical commentary can only include a negative recommendation, that means, not to follow those traces of interpretation but rather to defend and to consult the texts originally composed by Hegel.
Soberanía y guerra en la Filosofía del derecho de Hegel
Authors: Mizrahi Esteban
Published: April 2022

En este trabajo se intenta esclarecer los fundamentos conceptuales de la soberanía en la Filosofía del Derecho de Hegel con el propósito de brindar una respuesta a esta pregunta y estar así en condiciones de evaluar los alcances del soberanismo contemporáneo y lo ajustado de sus pretensiones. Como se verá, Hegel construye su propia posición en diálogo crítico con los abordajes de Hobbes y Kant, con referencias más o menos explícitas. El desarrollo de este artículo está dividido en tres partes. En la primera, (a) se presenta la concepción hegeliana de la soberanía en el marco de su comprensión organicista del Estado, atendiendo tanto a su aspecto interior como exterior. En la segunda, (b) se analizan los fundamentos teóricos de la guerra en Hegel y su posición respecto de las relaciones internacionales. Por último, (c) se discuten los alcances del soberanismo en la actualidad a la luz del pensamiento hegeliano.
Espiritualidade no ensino em saúde: scoping review
Authors: Silva Fabiana Taborda de Ramos da , Silveira Isabela Hodecker da , Guetter Rosiane Mello , Franco Renato Soleiman , Sanches Leide da Conceição
Published: April 2022

Scoping review to map scientific productions on spirituality in health education. The acronym Population Concept Context (PCC) - Population for undergraduate health students, Concept for spirituality and religion, and Context for health teaching. This study was directed by PRISMAScr and included articles published in Portuguese, Spanish, and English from January 2010 to September 2020. The initial search identified 798 articles of which 12 were included in the review. The main results reveal an increase in publications between 2010 e 2019 and indicate that among the 12 articles included in the study, eight contain methodological proposals for health education and spirituality, with a search for more appropriate methodologies and structures from a pedagogical point of view. It is concluded that bringing spirituality to health students allows self-reflection and training for the professors themselves.
New General Decay Result for a Class of Viscoelastic Pseudo-parabolic Equations with Critical Sobolev Exponent
Authors: Ngo Vu , Dao Dung
Published: April 2022

This work is concerned with a multi-dimensional viscoeastic
pseudo-parabolic equation with critical Sobolev exponent. First, with
some suitable conditions, we prove that the weak solution exists
globally. Next, we show that the stability of the system holds for a
much larger class of kernels than the ones considered in previous
literatures. More precisely, we consider the kernel
$g:\left[ {0,\infty }
\right) \longrightarrow
\left( {0,\infty }
\right)$ satisfying ${g^\prime
}\left( t \right)
\leqslant - \xi \left( t
\right)G\left( {g\left( t
\right)} \right)$, where
$\xi$ and $G$ are functions satisfying some specific
properties.
Thermal entry problem for Vo ̵́cadlo fluid model bounded within passive tube and channel with axial conduction and viscous dissipation: a Graetz-Nusselt problem
Authors: Khan Muhammad Waris , Ali Naisr , Asghar Zeeshan
Published: April 2022

The biomedical applications of the Graetz-Nusselt problem with
non-Newtonian properties are closely associated with heat transfer
processes in fluid flow systems. Inspired by the ubiquity of complex
rheological fluids in the thermal entry flow problem, we extend the
classical Graetz-Nusselt problem for Vo~̵́cadlo non-Newtonian fluid. This
analysis is also valid for Bingham viscoplastic liquid, power-law and
Newtonian fluids. The problem is solved by a semi-analytic technique.
For each fluid model, we obtain the closed-form solution of the velocity
field for both planner and cylindrical confinements. The separation of
variable approach is utilized to solve the energy equation along with
the Dirichlet boundary condition. Furthermore, to obtain the refined
eigenvalues (from Sturm-Liouville boundary value problem) MATLAB’s
built-in bvp4c function is utilized. The viscous dissipation (Brinkman
number) and axial conduction (Péclet number) effects on bulk temperature
and Nusselt numbers are also incorporated. The plots of Nusselt number
and mean temperature are presented for several values of yield stress
parameter, power-law index and Brinkman number with their detailed
explanation. The local Nusselt number for both geometries achieved fully
developed condition for the larger values of axial distance x~̵̅. The
current investigation can be applied in the field of nanotechnology,
mechanical and biomedical engineering. Designing several thermal types
of equipment and microfluidic devices also falls in the scope of this
study.
Identification of RCC1- LCK as a novel fusion gene in pediatric erythroid sarcoma
Authors: Oya Satoru , Osone Shinya , Yoshida Masanori , Nishimoto Sota , Taura Yoshihiro , Yoshida Hideki , Miyachi Mitsuru , Inaba Tohru , Konishi Eiichi , Kato Motohiro , Imamura Toshihiko , Iehara Tomoko
Published: April 2022

Erythroid sarcoma is very rare form of pure erythroid leukemia with
undetermined biological features. Here, we present an infant with a
multifocal erythroid sarcoma, diagnosed because the tumor cells were
positive for glycophorin A. After acute myeloid leukemia-oriented
chemotherapy and surgical resection followed by cord blood
transplantation, he has successfully maintained complete remission
without any late effects. Total transcriptome analysis of the tumor
identified a novel fusion gene, RCC1-LCK, and high LCK expression
levels, suggesting that LCK overexpression was involved in
leukemogenesis in this case.
Serosurvey and Risk Factors of Coxiella burnetii in Sheep and Goats in three agricultural zones of Borno State, Nigeria
Authors: Adamu G , Kia G. , Saidu S.N.A , Ejeh F.
Published: April 2022

Coxiellosis (Q-fever) caused by Coxiella burnetii is a
cosmopolitan zoonosis that causes significant losses through abortions
and stillbirths in small ruminants. A cross-sectional seroprevalence
study was conducted in three agricultural zones of Borno State in the
flocks of sheep and goats. Seven hundred sixty-eight small ruminants
(384 sheep and goats each) of both sexes (282 males and 486 females) of
different age groups from 90 flocks were randomly selected to collect
sera and related epidemiological data information. A commercial indirect
enzyme-linked immunosorbent assay (iELISA; I.D. Vet) was used to test
the samples for the presence of both phases I and II antibodies to
Coxiella burnetii infections. The seroprevalence in sheep and
goats was 10.9% (44/384) and 12.0% (46/384). There was no
statistically significant association in the two species of the animals
tested and the infection rates of coxiellosis. Bivariate analysis showed
that female animals of all species tested were more seropositive for
antibodies to Coxiella burnetii than males. There was a
statistically significant association between the sex of both sheep and
goats tested and the infection rates of coxiellosis. Breed and age of
sheep and goats were not statistically significant for seropositivity to
Coxiella burnetii. The study indicates that seroprevalence of
coxiellosis was high in the studied small ruminants’ population,
particularly among female sheep and goats and can be considered a
potential risk for both susceptible animals and humans in the study
area.
Evaluación de la digestibilidad in vivo en conejos utilizando Tithonia diversifolia como remplazo parcial del concentrado
Authors: Chinchilla Silva Erika Nathaly , Roa Vega María Ligia
Published: April 2022

La producción de conejos en Colombia es un sistema de producción poco explotado hasta el momento, pero que día a día ha ido tomando más auge por la facilidad de su establecimiento, sus altos índices productivos y su gran aporte nutricional a la alimentación humana, pero su establecimiento en el país se ha visto obstaculizado por los altos costos de los insumos utilizados para su alimentación, ya que en su gran mayoría son importados y empleados para la elaboración de concentrados, por esta razón es necesario buscar estrategias de alimentación, que satisfagan los requerimientos nutricionales de los animales y que sean de fácil acceso y económicos para el productor. El presente estudio tuvo como objetivo, evaluar la digestibilidad in vivo en conejos utilizando Tithonia diversifolia como remplazo parcial del concentrado, usando como parámetros de evaluación el porcentaje de nutrientes digestibles totales (NDT), coeficientes de digestibilidad (Cod) de proteína cruda (PC), fibra cruda (FC), extracto etéreo (EE) y extracto no nitrogenado (ENN); energía metabolizable (EM) y energía digestible (ED) para cada uno de los tratamientos; usando como unidad experimental, 24 conejos con un peso promedio de 2 kg ± 300 gr, distribuidos por un diseño completamente al azar, de la siguiente manera, el T0 fue el control, con dieta basal 100% concentrado;T1 remplazo del 10% del concentrado por Tithonia diversifolia; T2 remplazo del 20% y T3 remplazo del 30%. De donde se concluyó que la inclusión de Botón de oro en todos los tratamientos, presentó un aporte de nutrientes concordantes con lo requeridos por los conejos y dichos nutrientes presentaron una buena digestibilidad, lo cual constituye un recurso alimenticio alternativo en su alimentación en condiciones tropicales, con el fin de disminuir los costos de producción y mantener los rendimientos.