bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling the Differentiation Potential of Ovarian Theca Interna Cells from Multipotent Stem Cell-like Cells
Authors: Vlieghe Hanne , Sousa Maria João , Charif Dania , Amorim Christiani A.
Published: July 2024

Research question: Theca interna cells (TICs) are an indispensable cell source for ovarian follicle development and steroidogenesis. Recent studies have identified theca stem cells (TSCs) in both humans and animals. Interestingly, TSCs express mesenchymal stem cell (MSC)-related markers and can differentiate into mesenchymal lineages. MSCs are promising for tissue engineering and regenerative medicine due to their self-renewal and differentiation abilities. Therefore, this study investigated the potential origin of TICs from MSCs. Design: Whole ovaries from postmenopausal organ donors were obtained, and their cortex was cryopreserved prior to the isolation of stromal cells. These isolated cells were differentiated in vitro to TICs using cell media enriched with various growth factors and hormones. Immunocytochemistry, an enzyme-linked immunosorbent assay, flow cytometry, and reverse transcription–quantitative polymerase chain were employed at different timepoints. Data were analyzed using one-way ANOVA. Results: Immunocytochemistry showed an increase in TIC markers from day 0 to day 8 and a significant rise in MSC-like markers on day 2. This corresponds with rising androstenedione levels from day 2 to day 13. Flow cytometry identified a decreasing MSC-like cell population from day 2 onwards. The CD13+ cell population and its gene expression increased significantly over time. NGFR and PDGFRA expression was induced on days 0 and 2, respectively, compared to day 13. Conclusions: This study offers insights into MSC-like cells as the potential origin of TICs. Differentiating TICs from these widely accessible MSCs holds potential significance for toxicity studies and investigating TIC-related disorders like polycystic ovary syndrome (PCOS).
Therapeutic Potential of Olfactory Ensheathing Cells and Adipose-Derived Stem Cells in Osteoarthritis: Insights from Preclinical Studies
Authors: Chang Yu-Hsun , Wu Kun-Chi , Hsu Chih-Jung , Tu Tsui-Chin , Liu Mei-Chun , Chiang Raymond Yuh-Shyan , Ding Dah-Ching
Published: July 2024

Olfactory-ensheathing cells (OECs) are known for their role in neuronal regeneration and potential to promote tissue repair. Adipose-derived stem cells (ADSCs), characterized by mesenchymal stem cell (MSC) traits, display a fibroblast-like morphology and express MSC surface markers, making them suitable for regenerative therapies for osteoarthritis (OA). In this study, OECs and ADSCs were derived from tissues and characterized for their morphology, surface marker expression, and differentiation capabilities. Collagenase-induced OA was created in 10-week-old C57BL/6 mice, followed by intra-articular injections of ADSCs (1 × 105), OECs (1 × 105), or a higher dose of OECs (5 × 105). Therapeutic efficacy was evaluated using rotarod performance tests, MRI, histology, and immunohistochemistry. Both cell types exhibited typical MSC characteristics and successfully differentiated into adipocytes, osteoblasts, and chondrocytes, confirmed by gene expression and staining. Transplantation significantly improved rotarod performance and preserved cartilage integrity, as seen in MRI and histology, with reduced cartilage destruction and increased chondrocytes. Immunohistochemistry showed elevated type II collagen and aggrecan in treated joints, indicating hyaline cartilage formation, and reduced MMP13 and IL-1β expression, suggesting decreased inflammation and catabolic activity. These findings highlight the regenerative potential of OECs and ADSCs in treating OA by preserving cartilage, promoting chondrocyte proliferation, and reducing inflammation. Further research is needed to optimize delivery methods and evaluate long-term clinical outcomes.
Cutaneous Adverse Reactions and Survival Outcomes of Advanced Melanoma Treated with Immune Checkpoint Inhibitors in an Academic Medical Centre in Singapore
Authors: Lim Agnes Yeok-Loo , Chan Jason Yongsheng , Oh Choon Chiat
Published: July 2024

Programmed cell death-1 (PD1) inhibitors, a form of immune checkpoint inhibitor, are efficacious for metastatic melanoma but are associated with cutaneous adverse reactions (CARs). Studies in Europe and North America showed that CARs are associated with an increased overall survival. However, studies from Asia showed mixed results. There is a paucity of data regarding the efficacy of PD1 inhibitors and the effect of CARs on overall survival from Southeast Asia. A retrospective study of patients in the National Cancer Centre Singapore who were diagnosed with melanoma between 2015 and 2020 was conducted. Patients were included in the study if they had stage IV melanoma (advanced melanoma). Sixty-two patients were included in the study. The median age was 62.5 years and acral melanoma was the commonest subtype. Forty-three patients received PD1 inhibitors. Comparing patients who did not receive PD1 inhibitors to patients who received PD1 inhibitors, the former had a median overall survival of 6 months (95% CI: 5.07, 6.93), whereas the latter had a median overall survival of 21 months (95% CI: 13.33, 28.67; p < 0.001) (Hazard ratio 0.32; 95% CI: 0.16, 0.63; p = 0.001). Amongst patients who received PD1 inhibitors, patients who developed CARs had a greater median overall survival of 33 months (95% CI: 17.27, 48.73) compared to 15 months (95% CI: 9.20, 20.80; p = 0.013) for patients who did not (HR 0.29; 95% CI: 0.098, 0.834; p = 0.022). This study provides insight into the outcomes of metastatic melanoma in Singapore, and adds to the body of evidence supporting the use of PD1 inhibitors in Asians.
The NaviSight Study: Investigating How Diabetic Retinopathy and Retinitis Pigmentosa Affect Navigating the Built Environment
Authors: Cushley Laura N. , Csincsik Lajos , Virgili Gianni , Curran Katie , Silvestri Giuliana , Galway Neil , Peto Tunde
Published: July 2024

Background: Visual impairment is a global problem and, regardless of the cause, it substantially impacts people’s daily lives. Navigating towns and cities can be one of the most difficult tasks for someone with a visual impairment. This is because our streetscapes are often inaccessible for navigating safely and independently by people with a visual impairment. Barriers include street clutter, bollards, pavement parking, and shared spaces. Methodology: Participants with varying levels of diabetic retinopathy (DR) and retinitis pigmentosa (RP) were recruited. Each participant completed a clinical visit and a 1-mile walk. Participants discussed confidence, anxiety, difficulty, and any barriers encountered while completing the walkaround. Participants completed quality of life (RetDQol), diabetes distress scales, and a study questionnaire. They also underwent retinal imaging and visual function testing. Retinal imaging and visual function results were compared with confidence, difficulty, and anxiety levels during the walkaround using Spearman’s correlation. Results: Thirty-three participants took part in the study, 22 with diabetes and 11 with RP. Results showed that average confidence was correlated with visual acuity, RetDQol, mean visual fields, and vertical peripheral diameter visual fields. Average difficulty was associated with visual acuity, RetDQol, dark adaptation, mean visual fields, percentage of the retina, and both horizontal and vertical diameter visual fields. In addition, some of the barriers discussed were pavement issues, bollards, parked cars, uneven pavements, alfresco dining, light levels, and street features such as tree roots, poles, A-boards, and street clutter. Conclusions: People with RP and treated DR faced common barriers while navigating the walkaround. The removal of these common barriers would make our streetscapes more accessible for all and will allow for more independence in those with visual impairments.
The Perceptions of University Students as to the Benefits and Barriers to Using Immersive Virtual Reality in Learning to Work with Individuals with Developmental Disabilities
Authors: Luke Nicole , Keith Avery , Bajcar Nicole , Sureshkumar Brittney , Adebayo Oluwakemi
Published: July 2024

The aim of this study is to understand the experiences of university students who took part in a pilot program for an experiential learning opportunity in immersive virtual reality (iVR). Experiential learning opportunities are essential for students who will be expected to apply their knowledge in a professional setting. Head-mounted display devices were distributed to university students and individuals with developmental disabilities at a partnering community organization. The university students met community partners in a virtual world and interacted with them to learn about their partners’ self-selected goals related to communication and job skills. A mixed methods analysis of survey responses and journal entries was conducted. Students reported an overall positive experience with iVR and indicated an interest in pursuing future opportunities to include iVR in their learning.
Two-Dimensional Direction-of-Arrival Estimation Using Direct Data Processing Approach in Directional Frequency Analysis and Recording (DIFAR) Sonobuoy
Authors: Nemati Amirhossein , Zakeri Bijan , Molaei Amir Masoud
Published: July 2024

Today, the common solutions for underwater source angle detection require manned vessels and towed arrays, which are associated with high costs, risks, and deployment difficulties. An alternative solution for such applications is represented by acoustic vector sensors (AVSs), which are compact, lightweight and moderate in cost, and which have promising performance in terms of the bearing discrimination in two or three dimensions. One of the most popular devices for passive monitoring in underwater surveillance systems that employ AVSs is the directional frequency analysis and recording (DIFAR) sonobuoy. In this paper, direct data-processing (DDP) algorithms are implemented to calculate the azimuth angle of underwater acoustic sources by using short-time Fourier transform (STFT) via the arctan method instead of using fast Fourier transform (FFT). These algorithms for bearing estimation use the ‘Azigram’ to plot the estimated bearing of a source. It is demonstrated that by knowing the active sound intensity of the sound field and applying the inverse tangent to its real part, this matrix can be obtained. Announcing the time and frequency of the source simultaneously is one of the main advantages of this method, enabling the detection of multiple sources concurrently. DDP can also provide more details about sources’ characteristics, such as the frequency of the source and the time of the source’s presence.
Design Optimization of an Enhanced-Mode GaN HEMT with Hybrid Back Barrier and Breakdown Voltage Prediction Based on Neural Networks
Authors: Tian Kuiyuan , Hu Jinwei , Du Jiangfeng , Yu Qi
Published: July 2024

To improve the breakdown voltage (BV), a GaN-based high-electron-mobility transistor with a hybrid AlGaN back barrier (HBB-HEMT) was proposed. The hybrid AlGaN back barrier was constructed using the Al0.25Ga0.75N region and Al0.1G0.9N region, each with a distinct Al composition. Simulation results of the HBB-HEMT demonstrated a breakdown voltage (1640 V) that was 212% higher than that of the conventional HEMT (Conv-HEMT) and a low on-resistance (0.4 mΩ·cm2). Ultimately, the device achieved a high Baliga’s figure of merit (7.3 GW/cm2) among reported devices of similar size. A back-propagation (BP) neural network-based prediction model was trained to predict BV for enhanced efficiency in subsequent work. The model was trained and calibrated, achieving a correlation coefficient (R) of 0.99 and a prediction accuracy of 95% on the test set. The results indicated that the BP neural network model using the Levenberg–Marquardt algorithm accurately predicted the forward breakdown voltage of the HBB-HEMT, underscoring the feasibility and significance of neural network models in designing GaN power devices.
Data-Driven Rotary Machine Fault Diagnosis Using Multisensor Vibration Data with Bandpass Filtering and Convolutional Neural Network for Signal-to-Image Recognition
Authors: Łuczak Dominik
Published: July 2024

This paper proposes a novel data-driven method for machine fault diagnosis, named multisensor-BPF-Signal2Image-CNN2D. This method uses multisensor data, bandpass filtering (BPF), and a 2D convolutional neural network (CNN2D) for signal-to-image recognition. The proposed method is particularly suitable for scenarios where traditional time-domain analysis might be insufficient due to the complexity or similarity of the data. The results demonstrate that the multisensor-BPF-Signal2Image-CNN2D method achieves high accuracy in fault classification across the three datasets (constant-velocity fan imbalance, variable-velocity fan imbalance, Case Western Reserve University Bearing Data Center). In particular, the proposed multisensor method exhibits a significantly faster training speed compared to the reference IMU6DoF-Time2GrayscaleGrid-CNN, IMU6DoF-Time2RGBbyType-CNN, and IMU6DoF-Time2RGBbyAxis-CNN methods, which use the signal-to-image approach, requiring fewer iterations to achieve the desired level of accuracy. The interpretability of the model is also explored. This research demonstrates the potential of bandpass filters in the signal-to-image approach with a CNN2D to be robust and interpretable in selected frequency bandwidth machine fault diagnosis using multiple sensor data.
Soil as a Source of Fungi Pathogenic for Public Health
Authors: Grishkan Isabella
Published: July 2024

Soil is an environment for huge diversity of fungi, which fulfill various tasks and support the maintaining of soil health. At the same time, varieties of soil fungal species, which produce numerous airborne spores and a range of mycotoxins, are known to be pathogenic for human health. The present review aims to summarize the current knowledge on soil fungi causing public health problems, including dermatoses, allergies, pulmonary diseases, wound infections, infections of the central nervous system, etc.
Pectins Rich in RG-I Extracted from Watermelon Peel: Physicochemical, Structural, Emulsifying, and Antioxidant Properties
Authors: Ma Xiaojun , Cheng Xinxin , Du Yuyi , Tang Peiyao , Chen Liangxiao , Chen Wei , Zheng Zhenjia
Published: July 2024

RG-I pectin has excellent health benefits, but its raw materials are relatively scarce, and its complex structure often breaks down its side-chain structure during the extraction process. In this study, the physicochemical and antioxidant properties of a branched-chain-rich pectin gained from watermelon peel were demonstrated, and the structure–function relationships of RG-I-enriched pectin and emulsification properties were investigated. Fourier transform infrared spectroscopy, high-performance anion exchange chromatography, high-performance gel permeation chromatography, nuclear magnetic resonance spectroscopy, and methylation analyses reveal it as acetylated, low-methoxylated pectin, rich in RG-I side chains (MW: 1991 kDa, RG-I = 66.17%, methylation degree: 41.45%, (Ara + Gal)/Rha: 20.59%). RPWP outperforms commercial citrus pectin in emulsification and stability, significantly preventing lipid oxidation in emulsions. It also exhibits free radical scavenging abilities, contributing to its effectiveness in preventing lipid oxidation. Emulsions made with RPWP show higher viscosity and form a weak gel network (G′ > G″), enhancing stability by preventing phase separation. These findings position watermelon peel as a good source of RG-I pectin and deepen our understanding of RPWP behavior in emulsion systems, which may be useful in the food and pharmaceutical fields.
Predictors of Meat Reduction: The Case of Slovenia
Authors: Kirbiš Andrej , Korže Vanesa , Lubej Maruša
Published: July 2024

Health, environmental, and animal advocacy organisations emphasise reducing or eliminating high meat consumption due to its adverse effects on health, sustainability, climate change, and animal welfare. Increasingly, people are deciding to reduce their meat consumption frequency. Our study aimed to examine predictors of meat reduction among Slovenian consumers, focusing on gender, age, partner and children status, size of residential settlement, socioeconomic status, and political orientation. We conducted a survey using non-probability sampling. We examined demographic, socioeconomic, and political predictors of individuals’ self-assessed intent to reduce meat consumption in the month following the survey. Additionally, we analysed respondents’ meat reduction during the three years prior. A correlation analysis revealed that higher age and education levels were significantly positively correlated with meat reduction patterns. An ordinal logistic regression analysis indicated that age was the only significant predictor of meat reduction intentions and past behaviour. Our findings suggest that middle-aged and elderly individuals are more likely than younger adults to report meat reduction behaviours. Future public health interventions should tailor approaches to different age groups, and, in particular, target younger individuals. Educational campaigns should highlight the health and environmental benefits of reducing meat and animal product consumption, particularly in primary and secondary schools.
Understanding the Factors Driving Consumers’ Willingness to Pay for Gene-Edited Foods in China
Authors: Gao Shuqing , Chen Jingru , Yang Yuqin , Wang Guoyan
Published: July 2024

Gene editing contributes to enhancing food security through the creation of novel foods. However, public perception of gene-edited (GE) foods is crucial to their acceptance and adoption. This study expanded the knowledge–attitude–practice model and constructed an integrated framework comprising four dimensions: demographic factors, scientific literacy and beliefs, social trust, and perceptions of gene technology, aimed at explaining the public’s attitudes toward GE foods. A questionnaire survey was conducted (N = 649), revealing a positive attitude toward GE foods, with over 80% expressing a certain willingness to pay (WTP) for them. Factors such as income level, subjective knowledge, scientific beliefs, trust in scientists, trust in government, and trust in national technological capabilities and perceived benefits positively correlated with WTP. Conversely, objective knowledge, perceived risks, and perceived ethical concerns were negatively correlated with WTP. The impact of objective knowledge on attitudes toward GE foods demonstrated a significant, nonlinear relationship. Additionally, it is noteworthy that the Chinese public currently exhibits relatively low trust in national technological capabilities, necessitating vigilance against the emergence of conspiracy theories akin to those surrounding genetically modified foods. This research contributes theoretical insights into the public communication of GE foods.
“Except for This Hysteria, She Is the Perfect Woman”: Women and Hysteria in An Inconvenient Wife
Authors: Voigt Nina Marie
Published: July 2024

Historical fiction can be understood as a hybrid space: it represents the past and simultaneously allows a consideration of the culture it is written in. Under the assumption that novels help address cultural shifts and attitudes, this paper aims to investigate how, why, and with what implications medical discourses surrounding women are depicted in fiction. This paper explores the manifold conceptualizations of hysteria in An Inconvenient Wife written by Megan Chance in 1998, arguing that the novel presents a complex view of discourses of medicalization. Its central claim is that the novel constructs hysteria not only as a tool of oppression but also as a tool with which to escape social constraints and patriarchal control. Through understanding historical fiction as not merely commenting on the past, but as addressing contemporary issues, the text adds to discussions centering on intersections of medicine and literature.
A Mobile Application for Enhancing Caregiver Support and Resource Management for Long-Term Dependent Individuals in Rural Areas
Authors: Turnbull Niruwan , Sriruecha Chanaphol , Kongmant Ruchakron , Nghiep Le Ke , Tudpor Kukiat
Published: July 2024

The “SmartCaregivers” 1.0 mobile application is a beacon of hope for caregivers (CG) in rural areas, often facing limited access to facilities and support. This study, conducted from February to August 2021, aimed to comprehensively analyze the need for developing a database system and a mobile application tailored to enhance caregiver support and resource management for long-term dependent individuals in the rural areas of Maha Sarakham province, Thailand. The research followed a rigorous research and development (R & D) approach, specifically the ADDIE model (analysis, design, development, implementation, and evaluation). Data were collected from 402 caregivers and 10 key informants through surveys and interviews, as well as from 402 caregivers during the implementation and evaluation phases. The application’s impact was assessed using a quasi-experimental design with a one-group pre–post-test, and its acceptance was evaluated through the technology acceptance model (TAM). The application significantly improved caregivers’ knowledge scores, with a mean increase from 10.49 ± 2.53 to 12.18 ± 2.76 post-intervention. High scores for perceived usefulness (4.36 ± 0.62) and ease of use (4.31 ± 0.59) reassure the audience about the application’s effectiveness in providing rapid access to health information, aiding decision-making, and improving care coordination. The system quality was also highly rated, with users appreciating the variety of functions and structural design. This potential for transformation and improvement instills hope and optimism for the future of caregiving in rural areas.
Community-Based Interventions in People with Palliative Care Needs: An Integrative Review of Studies from 2017 to 2022
Authors: Vélez-López Antonia , Carmona-Torres Juan Manuel , López-González Ángel , Laredo-Aguilera José Alberto , Callado-Pérez David , Rabanales-Sotos Joseba
Published: July 2024

Aim: To describe the latest scientific evidence regarding community-based interventions performed on patients in need of palliative care worldwide. Introduction and background: Given the rise of chronic diseases, their complexities and the fragility of patients, we are facing around 56.8 million people in need of palliative care. Community-based healthcare, particularly palliative care, can address social inequalities and improve the biopsychosocial health of disadvantaged populations. Therefore, primary care, as the main health referent in the community, has a central role in the care of these patients. Methods: This is an integrative review from January 2017 to June 2022 that follows the PRISMA statement and has been registered in PROSPERO. PubMed, Cuiden, the Web of Science (WoS), Cochrane and LILACS were the five databases searched. The scientific quality assessment of the articles was carried out following the CASPe methodology. Study selection was carried out by two researchers, A.V.L. and J.M.C.T., using the inclusion and exclusion criteria mentioned below. In cases of doubt or discrepancy, a third author (J.R.S.) was consulted. Results: The interventions mentioned in the 16 articles analysed were classified under the following categories: music therapy, laughter therapy, spiritual and cognitive interventions, aromatherapy, interdisciplinary and community-based teams, advance care planning and community, volunteering, telemedicine and care mapping. Example: Educating people to talk about different ethical issues could improve their quality of life and help develop more compassionate cities. Conclusions: We have identified interventions that are easily accessible (laughter therapy, telemedicine or music therapy), simple enough to be carried out at the community level and do not incur high costs. This is why they are recommended for people with palliative care needs in order to improve their quality of life.
Algal Adaptation to Environmental Stresses: Lipidomics Research
Authors: Chadova Ksenia
Published: July 2024

Algal lipidomics is a new field of research that is gaining increasing popularity. The use of high-performance liquid chromatography–mass spectrometry (HPLC-MS) has made it possible to accurately determine the structure of each lipid molecule in a sample. Since algae are considered as a promising source of various compounds with pharmacological and biotechnological potential, including bioactive lipids and polyunsaturated fatty acids, lipidomics research of this group of organisms are of particular interest. The algae lipidome has high plasticity, which is due to the influence of abiotic and biotic environmental factors, and the observed changes in lipid composition are, as a rule, adaptive reactions. This review examines current research in the field of algal lipidomics, discusses the results of studying the influence of various environmental factors, such as temperature, light intensity, nutrient concentration, epi- and endophytic infections on the algae lipidome, and seasonal and geographical plasticity of algae lipidome; questions about the adaptation mechanisms of algae at the level of individual lipid molecular species are considered, and gaps in this area of research are noted.
Early Diagnosis of Amyloidosis and Cardiac Involvement through Carpal Tunnel Surgery and Predictive Factors
Authors: Navarro-Saez María del Carmen , Feijoo-Massó Carlos , Berenguer Sánchez Alex , Parra Parente Tamara , Guillamon Toran Laura , Marcano-Fernández Francesc , Camara-Cabrera Jaume , Bravo Ferrer Zully del Carmen , Comet Monte Ricard , Calvet Calvo Xavier
Published: July 2024

Background/Objectives: To determine the prevalence of amyloidosis through the analysis of synovial tissue and transverse carpal ligament (TCL) in patients undergoing surgery for carpal tunnel syndrome (CTS), detect predictive factors for the presence of amyloid, and assess cardiac involvement degree. Methods: A prospective study with longitudinal cohort follow-up at a teaching hospital. Patients undergoing CTS surgery from 1 January 2019 to 31 May 2021 were included. Samples from synovial and TCL tissues were examined for amyloid presence. Multivariate analysis was used to detect predictive factors of the presence of amyloid. Patients with amyloid underwent echocardiography, laboratory analyses, and scintigraphy. Results: Two hundred and forty-six patients were included. The prevalence of amyloid was 11.4% in TCL and 12.6% in synovial tissues. Age (p = 0.035; OR 1.123), bilateral CTS symptoms (p = 0.022; OR 3.647), and trigger finger (p < 0.001; OR 3.537) were predictors of the presence of amyloid. Seventeen patients were diagnosed with transthyretin amyloidosis (ATTR) located in the carpus (no scintigraphic cardiac uptake or grade 0), one with light chain amyloidosis, eight with ATTR with cardiac involvement (grades 2–3), and five with ATTR in the carpus and scintigraphic uptake grade 1 (with normal echocardiogram and blood and urine tests). Conclusions: We detected amyloid in 12.6% of unselected consecutive patients who underwent CTS surgery. Biopsy in patients with CTS for amyloid detection, especially in elderly patients with bilateral symptoms and trigger finger, may be useful for the early diagnosis of amyloidosis, primarily due to transthyretin.
A Rare Case of Bullous Morphea Associated with Autoimmune Hepatitis
Authors: Jokubaitė Jorinta , Klapatauskaitė Goda , Macejevska Monika Marta , Grigaitienė Jūratė
Published: July 2024

(1) Background: Bullous morphea is an extremely rare form of localized scleroderma, a condition that is marked by the presence of sporadic and intermittent blisters on sclerodermatous skin. This condition stands out due to its rarity and the unique manifestation of blistering, which sets it apart from other forms of localized scleroderma. Due to the infrequent presentation of bullous morphea, there is a significant gap in our understanding of its pathogenesis. The exact mechanisms that lead to the development of this condition remain largely unknown, which poses a challenge for medical professionals in terms of both diagnosis and treatment. The limited number of reported cases makes it difficult to establish a standardized approach to managing this condition, and as a result, treatment options are often limited and may vary from one patient to another. (2) Methods: In this case report, we present a rare case of bullous morphea that manifested before the onset of autoimmune hepatitis. When morphea presents unusually or is resistant to traditional immunosuppressive treatment, a comprehensive assessment of possible concurrent autoimmune illnesses provoking the rash must be conducted. (3) Results: We report a successful case of bullous morphea treated with systemic corticosteroids following a diagnosis of autoimmune hepatitis. (4) Conclusions: This case highlights the importance of considering overlapping autoimmune conditions in the management of bullous morphea and the potential efficacy of systemic corticosteroids in such scenarios. Collaborative efforts involving dermatologists, rheumatologists, and hepatologists are essential to enhance understanding and optimize treatment outcomes for patients affected by this rare and complex condition. Thus, further research is necessary to gain a deeper understanding of the pathogenesis of bullous morphea and to develop more effective and targeted treatment options for patients affected by this condition.
Identification of Catecholamine and Drug Target α2A-Adrenoceptor in Human Testis and Human Testicular Peritubular Cells
Authors: Harald Welter , Nicole Kreitmair , Michaela Schneider , Carola Herrmann , Nina Schmid , Youli Stepanov , Thomas Fröhlich , Frank-Michael Köhn , Ulrich Pickl , Matthias Trottmann , Artur Mayerhofer
Published: July 2024

Background: Clonidine has been used in clinical medicine, e.g., to treat high blood pressure and other conditions. Animal studies have linked its use to impairments of male reproductive functions, and although only a few reports exist for the human species, such actions may exist in man as well. The underlying reasons and, specifically, possible actions of clonidine at the level of the testis are not known. Introduction: Clonidine is an agonist at the α2A-adrenoceptor (ADRA2A), which, as data bank mining indicated, is expressed by several cells of the human testis. The human testis and most of its cells are, however, not readily accessible to experimental testing. Cells from the peritubular wall compartment (human testicular peritubular cells; HTPCs) are the exception. Methods and Results: As shown by immunohistochemical/immunocytochemical and PCR techniques these cells express ADRA2A and retain expression upon isolation and culture. When tested over a concentration range (1–1000 µM) and 24 h, clonidine did not visibly affect HTPC morphology but significantly stimulated IL6 mRNA levels in a concentration-dependent manner. ELISA measurements of cell culture supernatants confirmed a stimulatory action of clonidine (10 µM) on secreted IL6. When examined in collagen gel contraction assays of HTPCs, clonidine (10 µM) exerted a slight relaxing action, while a proteomic study revealed that clonidine (10 µM) did not significantly change cellular protein abundance of HTPCs after 24 h (data available via ProteomeXchange with identifier PXD052220). Conclusion: Thus, ADRA2A-bearing cells in the human testis are targets for catecholamines and drugs such as clonidine. The results of this HTPCs-focused study only show the tip of the iceberg. It is likely that catecholamines/catecholaminergic drugs have the potential to interfere with human testicular functions.
Current Challenges in Diagnosis and Treatment of Cardiovascular Disease
Authors: Samanidis George
Published: July 2024

Cardiovascular disease is a leading the cause of death worldwide among the various cardiac pathologies that directly or indirectly affect the quality of life of patients [...]
Small Fiber Neuropathy Associated with Post-COVID-19 and Post-COVID-19 Vaccination Arthritis: A Rare Post-Infective Syndrome or a New-Onset Disease?
Authors: Bandinelli Francesca , Nassini Romina , Gherardi Eleonora , Chiocchetti Barbara , Manetti Mirko , Cincotta Massimo , Nozzoli Filippo , Nucci Elena , De Logu Francesco , Pimpinelli Nicola
Published: July 2024

Post-COVID-19 (PC) and post-COVID-19 vaccination (PCV) syndromes are considered emergent multidisciplinary disorders. PC/PCV small fiber neuropathy (SFN) was rarely described and its association with undifferentiated arthritis (UA) was never defined. We aimed to evaluate PC/PCV-UA associated with the recent onset of severe lower limb paresthesia, compare SFN positive (+) to negative (−) patients, and evaluate changes in biomarkers in SFN+ during treatments. Nineteen PC/PCV-UA-patients with possible SFN underwent skin biopsy at the Usl Tuscany Center (Florence) early arthritis outpatient clinic from September 2021 to March 2024. Eight selected SFN+ were compared to ten SFN− patients. In SFN+ patients, baseline joint ultrasound (US), electromyography (EMG), optical coherence tomography (OCT), and skin biopsy were repeated at six months. Moreover, SFN+ patients were clinically assessed by a 0–10 numeric rating scale for neurological symptoms and DAS28/ESR up to 12 months follow-up. SFN+ patients showed a lower intraepidermal nerve fiber density at histopathological examination of skin biopsies and a higher frequency of OCT and EMG abnormalities in comparison to SFN− patients. In SFN+ patients, US and DAS28/ESR significantly improved, while intraepidermal nerve fiber density did not significantly change at the six-month follow-up. Fatigue, motor impairment, burning pain, brain fog, and sensitivity disorders decreased at long-term follow-up (12 months).
Research on the Impact of Landscape Planning on Visual and Spatial Perception in Historical District Tourism: A Case Study of Laomendong
Authors: Shao Lingfang , Ma Pengfei , Zhou Zijin
Published: July 2024

Exploring the impact of landscape planning on visual and spatial perception is particularly significant for historical district tourism. The existing literature offers limited insight into which historical landscapes most effectively influence tourists’ visual and spatial perceptions. Our study investigates this relationship within the cultural heritage context of Laomendong, a historical district in Nanjing, China. Utilizing the Stimulus–Organism–Response (SOR) theoretical framework, this research explored how the stylistic, symbolic, and spatial dimensions of historical landscapes influence tourists’ visual and spatial perceptions. Structural equation modeling (SEM) was used to test hypotheses concerning the relationships between historical landscapes, visual perception, spatial perception, and individual predispositions. The findings revealed that stylistic factors (material, color, and ornament), symbolic factors (landmark, relic, and sign), and spatial factors (openness, scale, and layout) significantly enhance visual perception and subsequently spatial perception. Visual perception plays a mediating role in the influence of historical landscapes on spatial perception. Individual predispositions, like sensation-seeking, intensify spatial perception, while destination familiarity surprisingly shows a negative effect, suggesting that over-familiarity might diminish the novelty and excitement of a spatial experience. These insights provide practical implications for the sustainable development of landscape planning in historical district tourism.
Effects of Urban Land-Use Planning on Housing Prices in Chiang Mai, Thailand
Authors: Lu Shichao , Zhang Zhihua , Crabbe M. James C. , Suntichaikul Prin
Published: July 2024

Chiang Mai is an emerging tourism-oriented city in Thailand. The booming tourism industry during the past decades has triggered significant expansion in its urban land area, resulting in a large number of newly-built residential communities appearing on unplanned land. In this study, we used multiscale geographically weighted regression (MGWR)-based hedonic price analysis to investigate 4624 housing transactions from 524 residential communities in Chiang Mai. This showed that the recent land-use planning in Chiang Mai has had unusual effects on housing prices; specifically, the effects of accessibility to hospitals, primary and secondary schools, green parks, and shopping malls could be ignored, demonstrating that local residents were well satisfied with land-use planning for high-quality medical and education sources and good living environments throughout the whole of Chiang Mai, and that no more land-use planning and investment on these facilities was needed. However, limited bus routes were only used for tourism and could not provide convenient routes for local residents, leading to their negative effects on housing prices in downtown areas, so the local government should lower the bus stop density in downtown areas and strengthen the transportation links between downtown areas and suburbs. Our study will not only support the urban land planning department of Chiang Mai to optimize residential communities and nearby facilities, but can also provide insights into housing price formation mechanisms in similar tourism-oriented cities in Thailand and beyond.
Stakeholder Participation in REDD+ Program: The Case of the Consultation Process in Laos
Authors: Sawathvong Soukphavanh , Hyakumura Kimihiko , Fujisaki Taiji
Published: July 2024

REDD+ aims to mitigate climate change by reducing deforestation and forest degradation in developing countries while ensuring social and environmental benefits through the involvement of diverse stakeholders. While several studies evaluate stakeholder participation in various aspects of REDD+, such as the level and type of stakeholder participation at both the project and national context level, there is a lack of research on how the government conducted the consultation meetings across all administrative levels. The objective of this study is to evaluate the participation of different stakeholders in REDD+ in Lao People’s Democratic Republic (Lao PDR), or Laos, funded by the World Bank, with a focus on analyzing government consultation processes to identify drivers of deforestation and forest degradation. The study applied both a literature review and online interviews, conducted with government officials and representatives of CSOs, which took place from March to April 2020. The results indicate significant involvement from cross-sectoral stakeholders. However, central government agencies and development partners dominated stakeholder participation, potentially influenced by government ownership. Non-state stakeholders, though invited to the consultations, had limited participation, highlighting the need for greater emphasis on their roles to ensure the integration of diverse interests and views in the REDD+ initiative.
Land Use Carbon Budget Pattern and Carbon Compensation Mechanism of Counties in the Pearl River Basin: A Perspective Based on Fiscal Imbalance
Authors: Fan Zhenggen , Xia Wentong , Yu Hu , Liu Ji , Liu Binghua
Published: July 2024

Implementing watershed carbon compensation under the constraint of fiscal imbalance is a crucial approach for China to achieve its “dual carbon” goals. Focusing on 257 counties within the Pearl River Basin (PRB), this paper first measured and modified the land use carbon emissions, carbon absorption, and the land use carbon budget (LUCB) from 2005 to 2020. Subsequently, their spatiotemporal patterns and the changes before and after the modifications were analyzed. Finally, this paper determined the subject–object, value, and priority order of carbon compensation using the modified LUCB as the baseline value, and a carbon compensation mechanism that combines vertical and horizontal directions was constructed. The following findings were obtained: (1) At the time scale, the proportion of construction land and forestland in the land use structure increased, and carbon emissions, carbon absorption, and the LUCB showed an overall upward trend from 2005 to 2020. (2) At the spatial scale, the areas with high carbon emissions and carbon deficits were mainly located in the lower reaches of the basin, whereas the areas with high carbon absorption and carbon surpluses were widely distributed in the upper and middle reaches. The carbon deficit in urban municipal districts and resource-based counties was relatively serious. (3) In 2020, the total amount of carbon compensation in the PRB was CNY −8088.61 million. The number of counties that needed to be paid and compensated was 75 and 182, respectively. The carbon compensation mechanism constructed in this paper can provide a reference for other countries and regions with financial imbalances to achieve regional carbon neutrality.
Factors Predicting Effectiveness of Eradication Therapy for Helicobacter pylori-Associated Dyspepsia Symptoms
Authors: Yasuda Kohei , Chinda Daisuke , Shimoyama Tadashi , Arai Tetsu , Akitaya Kazuki , Fujiwara Sae , Nomiya Hiroki , Sasaki Yoshio , Komai Kazuo , Sawada Yoshihiko , Saito Yoshiharu , Chiba Hironobu , Sakuraba Hirotake , Fukuda Shinsaku ,   the RINGO Study Group
Published: July 2024

Functional dyspepsia is distinguishable from Helicobacter pylori-associated dyspepsia. However, distinguishing H. pylori-associated dyspepsia from functional dyspepsia before H. pylori eradication is difficult. Therefore, in the present study, we aimed to investigate whether serum pepsinogen levels before H. pylori eradication are associated with the amelioration of dyspepsia after successful H. pylori eradication. Additionally, we examined the usefulness of serum pepsinogen levels and other factors in predicting dyspepsia outcomes. H. pylori eradication was effective in 14 patients (Responders) and ineffective in 19 patients (Non-responders). The pepsinogen I/II ratio in Responders (3.4 ± 1.2) and Non-responders (2.3 ± 1.0) differed significantly (p = 0.006). The optimal cut-off pepsinogen I/II value was 2.3. Multivariate logistic regression analysis showed that the adjusted odds ratio for Non-responders was 26.1 (95% confidence interval: 2.0–338.0, p = 0.012) for a pepsinogen I/II ratio ≤ 2.3 and 8.10 (95% confidence interval: 1.1–57.6, p = 0.037) for smoking habits. The pepsinogen I/II ratio and smoking habits were associated with the effects of H. pylori eradication on dyspeptic symptoms. Thus, the pepsinogen I/II ratio cut-off value can be used to identify patients likely to respond to H. pylori eradication after the resolution of dyspeptic symptoms.
Comparative Genome-Wide Analysis Underscores the Rapid Expansion of Cytochrome P450s for Secondary Metabolism in the Mycoparasite Pezizomycetes
Authors: Syed Puleng Rosinah , Padayachee Tiara , Gamede Philasande , Nkosi Bridget Valeria Zinhle , Nelson David R. , Karpoormath Rajshekhar , Syed Khajamohiddin
Published: July 2024

Mycoparasite secondary metabolites control fungal infections or diseases in agriculture and human health. Among genes involved in synthesizing secondary metabolites, cytochrome P450 monooxygenases (CYPs/P450s) play a key role in synthesizing and attributing diversity to the secondary metabolites. Despite the importance of P450s, a comparative analysis of P450s in mycoparasites has yet to be reported. This study is aimed at addressing this research gap. Genome-wide analysis of P450s in 43 fungi representing six fungal phyla and three distinct lifestyles, such as mycoparasitic (24 species), saprophytic (5 species), and ectomycorrhizal (14 species), revealed the expansion of P450s in Pezizomycete mycoparasites for the synthesis of secondary metabolites. The number of P450s and their families and subfamilies, the number of secondary-metabolite biosynthetic gene clusters (SMBGCs), and the number of P450s that are part of these SMBGCs were found to be highest in Pezizomycete mycoparasites compared to their counterparts of saprophytes and ectomycorrhiza, indicating P450s also play a key role in mycoparasitism. An analysis of P450 location as part of SMBGCs and the available literature on Pezizomycete P450s revealed that P450s play a key role in the synthesis of anti-fungal secondary metabolites such as trichothecene sesquiterpene, harzianum A, heptelidic acid, and gliotoxin. The mycoparasite Trichoderma virens Tv29.8 P450 CYP68Q3 is found to be a bifunctional enzyme with epoxidation and oxidation capability, and CYP5117A3 performs a Baeyer–Villiger oxidation reaction with regioselectivity. This study serves as a reference for future annotation of P450s in mycoparasites.
Exploring the Antibacterial Potential of Bile Salts: Inhibition of Biofilm Formation and Cell Growth in Pseudomonas aeruginosa and Staphylococcus aureus
Authors: Tyagi Anuradha , Kumar Vinay , Joshi Navneet , Dhingra Harish Kumar
Published: July 2024

Chronic infections often involve notorious pathogens like Pseudomonas aeruginosa and Staphylococcus aureus, demanding innovative antimicrobial strategies due to escalating resistance. This investigation scrutinized the antibacterial prowess of bile salts, notably taurocholic acid (TCA), ursodeoxycholic acid (UDCA), and ox bile salt (OBS), against these pathogens. Evaluations encompassed minimum inhibitory concentration (MIC) determination, scrutiny of their impact on biofilm formation, and anti-virulence mechanisms. UDCA exhibited the highest efficacy, suppressing S. aureus and P. aeruginosa biofilms by 83.5% and 78%, respectively, at peak concentration. TCA also significantly reduced biofilm development by 81% for S. aureus and 75% for P. aeruginosa. Microscopic analysis revealed substantial disruption of biofilm architecture by UDCA and TCA. Conversely, OBS demonstrated ineffectiveness against both pathogens. Mechanistic assays elucidated UDCA and TCA’s detrimental impact on the cell membrane, prompting the release of macromolecular compounds. Additionally, UDCA and TCA inhibited protease and elastase synthesis in P. aeruginosa and staphyloxanthin and lipase production in S. aureus. These results underscore the potential of UDCA and TCA in impeding biofilm formation and mitigating the pathogenicity of S. aureus and P. aeruginosa.
Carbon Nanodot–Microbe–Plant Nexus in Agroecosystem and Antimicrobial Applications
Authors: Prokisch József , Nguyen Duyen H. H. , Muthu Arjun , Ferroudj Aya , Singh Abhishek , Agrawal Shreni , Rajput Vishnu D. , Ghazaryan Karen , El-Ramady Hassan , Rai Mahendra
Published: July 2024

The intensive applications of nanomaterials in the agroecosystem led to the creation of several environmental problems. More efforts are needed to discover new insights in the nanomaterial–microbe–plant nexus. This relationship has several dimensions, which may include the transport of nanomaterials to different plant organs, the nanotoxicity to soil microbes and plants, and different possible regulations. This review focuses on the challenges and prospects of the nanomaterial–microbe–plant nexus under agroecosystem conditions. The previous nano-forms were selected in this study because of the rare, published articles on such nanomaterials. Under the study’s nexus, more insights on the carbon nanodot–microbe–plant nexus were discussed along with the role of the new frontier in nano-tellurium–microbe nexus. Transport of nanomaterials to different plant organs under possible applications, and translocation of these nanoparticles besides their expected nanotoxicity to soil microbes will be also reported in the current study. Nanotoxicity to soil microbes and plants was investigated by taking account of morpho-physiological, molecular, and biochemical concerns. This study highlights the regulations of nanotoxicity with a focus on risk and challenges at the ecological level and their risks to human health, along with the scientific and organizational levels. This study opens many windows in such studies nexus which are needed in the near future.