bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of endoscopic thyroidectomy by complete areola approach and conventional open surgery in the treatment of differentiated thyroid carcinoma: A retrospective study and meta-analysis
Authors: Yuan Yuquan , Sun Chenyu , Yin Tingjie , Shao Cong , Pan Bin , Lu Dengwei , Hou Shaodong , Lowe Scott , Bentley Rachel , Chen Shuya , Huang Christy , Cheng Ce , Li Yaru , King Bethany , Zhou Qin , Yan Cunye , Zhang Fan
Published: December 2022

Background

The feasibility of endoscopic thyroidectomy by complete areola approach (ETCA) remains controversial. This study was conducted by combining our clinical data with the data obtained from a systematic review literature search to examine the effectiveness and safety of ETCA compared with conventional open thyroidectomy (COT) in differentiated thyroid carcinoma (DTC).

Methods

A total of 136 patients with a diagnosis of DTC who underwent unilateral thyroidectomy with central neck dissection from August 2020 to June 2021 were enrolled. The enrolled patients were divided into the ETCA group (n = 73) and the COT group (n = 63). The operative time, intraoperative bleeding volume, number of removed lymph nodes, number of metastatic lymph nodes, postoperative drainage volume, length of postoperative hospital stay, postoperative parathyroid hormone (PTH) levels, and complications were analyzed. Then, a systemic review and comprehensive literature search were conducted by using PubMed, Google Scholar, Embase, Web of Science, CNKI, Wanfang, and VIP database up to June 2022. Review Manager software version 5.3 was used for the meta-analysis.

Results

The results of clinical data showed that there were significant differences between the two groups in the operative time, intraoperative bleeding volume, removed lymph nodes, and postoperative drainage volume. There were no statistical differences in the length of postoperative hospital stay, number of metastatic lymph nodes, postoperative PTH level, and complications. In the systematic review and meta-analysis, 2,153 patients from fourteen studies (including our data) were ultimately included. The results of the meta-analysis found that ETCA had a longer operative time, larger postoperative drainage volume, and lower intraoperative bleeding volume. In terms of the length of postoperative hospital stay, the number of removed lymph nodes, and surgical complications, there was no significant difference between the two groups.

Conclusion

ETCA poses lower surgical bleeding and better cosmetic appearance compared with COT, while the length of operation and postoperative drainage in ETCA is less favorable compared with COT. In addition, ETCA is not inferior to COT in terms of the postoperative hospitalization stay, the number of removed lymph nodes, and surgical complications. Given its overall advantages and risks, ETCA is an effective and safe alternative for patients with cosmetic concerns.
Food-borne zoonotic echinococcosis: A review with special focus on epidemiology
Authors: Alvi Mughees Aizaz , Alsayeqh Abdullah F.
Published: December 2022

Echinococcosis is a neglected, WHO-listed cyclozoonotic parasitic disease that is caused by a number of species belonging to the genus Echinococcus. This disease is widespread across the globe, resulting in heavy economic losses for farmers and cystic disease in aberrant human hosts. This review paper briefly discussed taxonomy, a brief history, the magnitude of economic losses, host spectrum and life cycle, risk factors, and clinical manifestations. Furthermore, the copro- and sero-ELISA-based prevalence of echinococcosis on different continents was summarized. Finally, the authors analyzed the frequency and use of molecular epidemiology in the taxonomy of Echinococcus species based on molecular markers. This review will serve as a quick reference to Echinococcus.
miRNA profiling in intrauterine exosomes of pregnant cattle on day 7
Authors: Zhai Yaying , Shi Qiaoting , Chu Qiuxia , Chen Fuying , Feng Yajie , Zhang Zijing , Qi Xinglei , Arends Danny , Brockmann Gudrun A. , Wang Eryao , Lyu Shijie
Published: December 2022

Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (p-value < 0.05, |log2(FoldChange)| > 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (PBenjamini-Hochberg < 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation.
Neural Aspects of Prospective Control through Resonating Taus in an Interceptive Timing Task
Authors: van der Weel F. R. (Ruud) , Sokolovskis Ingemārs , Raja Vicente , van der Meer Audrey L. H.
Published: December 2022

High-density electroencephalography from visual and motor cortices in addition to kinematic hand and target movement recordings were used to investigate τ-coupling between brain activity patterns and physical movements in an interceptive timing task. Twelve adult participants were presented with a target car moving towards a destination at three constant accelerations, and an effector dot was available to intercept the car at the destination with a swift movement of the finger. A τ-coupling analysis was used to investigate involvement of perception and action variables at both the ecological scale of behavior and neural scale. By introducing the concept of resonance, the underlying dynamics of interceptive actions were investigated. A variety of one- and two-scale τ-coupling analyses showed significant differences in distinguishing between slow, medium, and fast target speed when car motion and finger movement, VEP and MRP brain activity, VEP and car motion, and MRP and finger movement were involved. These results suggested that the temporal structure present at the ecological scale is reflected at the neural scale. The results further showed a strong effect of target speed, indicating that τ-coupling constants k and kres increased with higher speeds of the moving target. It was concluded that τ-coupling can be considered a valuable tool when combining different types of variables at both the ecological and neural levels of analysis.
Lipid Metabolism Heterogeneity and Crosstalk with Mitochondria Functions Drive Breast Cancer Progression and Drug Resistance
Authors: Azam Aurelien , Sounni Nor Eddine
Published: December 2022

Breast cancer (BC) is a heterogeneous disease that can be triggered by genetic alterations in mammary epithelial cells, leading to diverse disease outcomes in individual patients. The metabolic heterogeneity of BC enhances its ability to adapt to changes in the tumor microenvironment and metabolic stress, but unfavorably affects the patient’s therapy response, prognosis and clinical effect. Extrinsic factors from the tumor microenvironment and the intrinsic parameters of cancer cells influence their mitochondrial functions, which consequently alter their lipid metabolism and their ability to proliferate, migrate and survive in a harsh environment. The balanced interplay between mitochondria and fatty acid synthesis or fatty acid oxidation has been attributed to a combination of environmental factors and to the genetic makeup, oncogenic signaling and activities of different transcription factors. Hence, understanding the mechanisms underlying lipid metabolic heterogeneity and alterations in BC is gaining interest as a major target for drug resistance. Here we review the major recent reports on lipid metabolism heterogeneity and bring to light knowledge on the functional contribution of diverse lipid metabolic pathways to breast tumorigenesis and therapy resistance.
Microbiota in the Natural History of Pancreatic Cancer: From Predisposition to Therapy
Authors: Binda Cecilia , Gibiino Giulia , Sbrancia Monica , Coluccio Chiara , Cazzato Maria , Carloni Lorenzo , Cucchetti Alessandro , Ercolani Giorgio , Sambri Vittorio , Fabbri Carlo
Published: December 2022

Early microbiome insights came from gut microbes and their role among intestinal and extraintestinal disease. The latest evidence suggests that the microbiota is a true organ, capable of several interactions throughout the digestive system, attracting specific interest in the biliopancreatic district. Despite advances in diagnostics over the last few decades and improvements in the management of this disease, pancreatic cancer is still a common cause of cancer death. Microbiota can influence the development of precancerous disease predisposing to pancreatic cancer (PC). At the same time, neoplastic tissue shows specific characteristics in terms of diversity and phenotype, determining the short- and long-term prognosis. Considering the above information, a role for microbiota has also been hypothesized in the different phases of the PC approach, providing future revolutionary therapeutic insights. Microbiota-modulating therapies could open new issues in the therapeutic landscape. The aim of this narrative review is to assess the most updated evidence on microbiome in all the steps regarding pancreatic adenocarcinoma, from early development to response to antineoplastic therapy and long-term prognosis.
Preparation of CuS/PbS/ZnO Heterojunction Photocatalyst for Application in Hydrogen Production
Authors: Chiu Ming-Huan , Kuo Cheng-Ching , Huang Chao-Wei , Yang Wein-Duo
Published: December 2022

A hexagonal wurtzite ZnO photocatalyst was prepared via a precipitation method. CuS nanoparticles (NPs) and PbS quantum dots (QDs) were loaded onto ZnO via a hydrothermal method to obtain a CuS/PbS/ZnO heterojunction photocatalyst. The CuS/PbS/ZnO photocatalyst obtained via the abovementioned method has significant absorption capabilities in the ultraviolet to near-infrared spectral regions, and effectively reduced the recombination of electron–hole pairs during a photocatalytic reaction. Electron microscope images showed that in the CuS/PbS/ZnO photocatalyst prepared at 130 °C, the particle size of the PbS QDs was approximately 5.5–5.7 nm, and the bandgap determined from the Tauc plot was 0.84 eV; this catalyst demonstrated the best water splitting effect. Furthermore, after adding a 0.25 M mixed solution of Na2S and Na2SO3 as the sacrificial reagent in photocatalysis for 5 h, the hydrogen production efficiency from water splitting reached 6654 μmol g−1 h−1.
Cr-Zn/Ni-Containing Nanocomposites as Effective Magnetically Recoverable Catalysts for CO2 Hydrogenation to Methanol: The Role of Metal Doping and Polymer Co-Support
Authors: Sorokina Svetlana A. , Kuchkina Nina V. , Grigoriev Maxim E. , Bykov Alexey V. , Ratnikov Andrey K. , Doluda Valentin Yu. , Sulman Mikhail G. , Shifrina Zinaida B.
Published: December 2022

CO2 hydrogenation to methanol is an important process that could solve the problem of emitted CO2 that contributes to environmental concern. Here we developed Cr-, Cr-Zn-, and Cr-Ni-containing nanocomposites based on a solid support (SiO2 or Al2O3) with embedded magnetic nanoparticles (NPs) and covered by a cross-linked pyridylphenylene polymer layer. The decomposition of Cr, Zn, and Ni precursors in the presence of supports containing magnetic oxide led to formation of amorphous metal oxides evenly distributed over the support-polymer space, together with the partial diffusion of metal species into magnetic NPs. We demonstrated the catalytic activity of Cr2O3 in the hydrogenation reaction of CO2 to methanol, which was further increased by 50% and 204% by incorporation of Ni and Zn species, respectively. The fine intermixing of metal species ensures an enhanced methanol productivity. Careful adjustment of constituent elements, e.g., catalytic metal, type of support, presence of magnetic NPs, and deposition of hydrophobic polymer layer contributes to the synergetic promotional effect required for activation of CO2 molecules as well. The results of catalytic recycle experiments revealed excellent stability of the catalysts due to protective role of hydrophobic polymer.
Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis
Authors: Lu Jiangting , Liao Jinling , Qin Min , Li Hui , Zhang Qingyuan , Chen Yang , Cheng Jiwen
Published: December 2022

Infertility affects lots of couples, half of which are caused by male factors. The LanCL1 gene is highly expressed in testis specifically, which might affect the development of sperms. In order to understand the potential functions of the LanCL1 gene in the testis, this study was conducted with constructed transgenic LanCL1 knockout mice. The mouse breeding experiment, semen analysis and single-cell RNAseq of testicular tissue were performed. Results suggested that the LanCL1 gene would significantly influence the reproduction ability and sperm motility of male mice. Single-cell RNAseq also confirmed the high expression of the LanCL1 gene in the spermatocytes and spermatids. Downregulating the LanCL1 gene expression could promote M2 macrophage polarity to maintain testicular homeostasis. Moreover, the LanCL1 gene could affect both the germ cells and stromal cells through various pathways such as the P53 signaling and the PPAR signaling pathway to disturb the normal process of spermatogenesis. However, no effects of the LanCL1 gene in testosterone synthesis and serum testosterone level were shown. Further studies are needed to discuss the mechanisms of the LanCL1 gene in the various cells of the testis independently.
Selection of New Heat Treatment Conditions for Novel Electroless Nickel-Boron Deposits and Characterization of Heat-Treated Coatings
Authors: Vitry Véronique , Yunacti Muslum , Mégret Alexandre , Khalid Hafiza Ayesha , Staia Mariana Henriette , Montagne Alex
Published: December 2022

Electroless nickel-boron coatings were deposited from a novel deposition bath that was stabilizer-free. Those coatings were analyzed by DSC to determine the crystallization temperature of nickel-boride phases, and then the best heat treatment conditions for the new coatings were selected using the Knoop hardness test and XRD. The results of DSC analysis and XRD were coherent with the findings of previous studies, which shows that the new coating has a boron content lower than 4% wt. The maximum hardness was obtained after heat treatment at 300 °C for 4 h and reached 1196 ± 120 hk50, which is much higher than the as-deposited coating. The heat-treated coatings were then fully characterized using optical and scanning electron microscopy, as well tribological and corrosion tests. Various sliding tests (ball-on-disk and ball-on-flat configurations) were conducted to analyze the coefficient of friction (COF) and wear behavior of the coatings. The maximum von Mises stress was calculated, and values of 624 MPa and 728 MPa were obtained for the ball-on-disk and ball-on flat, respectively, at a depth close to 14 µm from the surface, confirming the suitability of the applied load. Abrasive, adhesive, and fatigue wear mechanisms were observed on the worn sample morphology using SEM. It has been determined that during the corrosion test the OCP and corrosion potential values for the heat-treated coating increase as compared with the as-deposited one, whereas its corrosion resistance decreases.
Host-Parasite Relationships of Quill Mites (Syringophilidae) and Parrots (Psittaciformes)
Authors: Marciniak-Musial Natalia , Skoracki Maciej , Kosicki Jakub Z. , Unsöld Markus , Sikora Bozena
Published: December 2022

The family Syringophilidae (Acari: Prostigmata) includes obligatory ectoparasites, which occupy feather quills from various parts of avian plumage, where they feed and reproduce. Our study was concerned with the global fauna of syringophilid mites associated with Psittaciformes, as well as host-parasite specificity and evolution. We assumed that the system composed of quill mites and parrots represents a model group that can be used in a broader study of the relationships between parasites and hosts. In total, we examined 1524 host individuals of parrots belonging to 195 species, 73 genera, and 4 families (which constitute ca. 50% of global parrot fauna) from all zoogeographical regions where Psittaciformes occur. Among them, 89 individuals representing 81 species have been infested by quill mites belonging to 45 species and 8 genera. The prevalence of host infestations by syringophilid mites varied from 2.8% to 100% (95% confidence interval (CI Sterne method) = 0.1–100). We applied a bipartite analysis to determine the parasite-host interaction, network indices, and host specificity at the species and whole network levels. The Syringophilidae-Psittaciformes network was composed of 24 mite species and 47 host species. The bipartite network was characterized by a high network level specialization H2′ = 0.98, connectance C = 0.89, and high modularity Q = 0.90, with 23 modules, but low nestedness N = 0.0333. Moreover, we reconstructed the phylogeny of the quill mites on the generic level, and this analysis shows two distinct clades: Psittaciphilus (Peristerophila + Terratosyringophilus) (among Syringophilinae subfamily) and Lawrencipicobia (Pipicobia + Rafapicobia) (among Picobiinae). Finally, the distributions and host-parasite relationships in the system composed of syringophilid mites and parrots are discussed.
Evaluation of Transport Properties and Energy Conversion of Bacterial Cellulose Membrane Using Peusner Network Thermodynamics
Authors: Ślęzak-Prochazka Izabella , Batko Kornelia M. , Ślęzak Andrzej
Published: December 2022

We evaluated the transport properties of a bacterial cellulose (BC) membrane for aqueous ethanol solutions. Using the Rr version of the Kedem–Katchalsky–Peusner formalism (KKP) for the concentration polarization (CP) conditions of solutions, the osmotic and diffusion fluxes as well as the membrane transport parameters were determined, such as the hydraulic permeability (Lp), reflection (σ), and solute permeability (ω). We used these parameters and the Peusner (Rijr) coefficients resulting from the KKP equations to assess the transport properties of the membrane based on the calculated dependence of the concentration coefficients: the resistance, coupling, and energy conversion efficiency for aqueous ethanol solutions. The transport properties of the membrane depended on the hydrodynamic conditions of the osmotic diffusion transport. The resistance coefficients R11r, R22r, and Rdetr were positive and higher, and the R12r coefficient was negative and lower under CP conditions (higher in convective than nonconvective states). The energy conversion was evaluated and fluxes were calculated for the U-, F-, and S-energy. It was found that the energy conversion was greater and the S-energy and F-energy were lower under CP conditions. The convection effect was negative, which means that convection movements were directed vertically upwards. Understanding the membrane transport properties and mechanisms could help to develop and improve the membrane technologies and techniques used in medicine and in water and wastewater treatment processes.
Smart Contract Vulnerability Detection Model Based on Siamese Network (SCVSN): A Case Study of Reentrancy Vulnerability
Authors: Guo Ran , Chen Weijie , Zhang Lejun , Wang Guopeng , Chen Huiling
Published: December 2022

Blockchain technology is currently evolving rapidly, and smart contracts are the hallmark of the second generation of blockchains. Currently, smart contracts are gradually being used in power system networks to build a decentralized energy system. Security is very important to power systems and attacks launched against smart contract vulnerabilities occur frequently, seriously affecting the development of the smart contract ecosystem. Current smart contract vulnerability detection tools suffer from low correct rates and high false positive rates, which cannot meet current needs. Therefore, we propose a smart contract vulnerability detection system based on the Siamese network in this paper. We improved the original Siamese network model to perform smart contract vulnerability detection by comparing the similarity of two sub networks with the same structure and shared parameters. We also demonstrate, through extensive experiments, that the model has better vulnerability detection performance and lower false alarm rate compared with previous research results.
Commutation Behavior and Stray Inductance Analysis of a FC-3L-BDC Phase-Leg PEBB
Authors: Liu Haitao , Xie Shunmeng , Dou Zechun , Qi Yu , Liu Feng , Tan Yifan
Published: December 2022

The bidirectional dc-dc converter is a critical component for extending the use of renewable energy and improving the efficiency of high-power electronic systems. This paper presents the analysis of the stray inductance of a commutation loop and the commutation behavior of IGBT devices in a flying capacitor three-level bidirectional DC-DC converter (FC-3L-BDC) phase-leg power electronic building block (PEBB). An FC-3L-BDC phase-leg PEBB was designed as an example, which can be used to build 400 kW to MW-grade light rail train chargers, battery energy storage interface converters, or metro regenerative braking energy recovery converters with a single PEBB or several PEBBs interleaved parallel. In order to optimize the stray inductance of commutation paths and realize snubberless operation, a five-layer laminated bus bar was carefully designed, and the stray inductance of the bus bar was extracted by three-dimensional finite element analysis simulation. To obtain higher accuracy, the stray inductances of IGBT devices and capacitors were extracted from the test instead of their datasheets. Then, the accuracy of the commutation loop stray inductance analysis method was verified by practical experiments. The impact of the stray inductance of the commutation loop on the commutation behavior of IGBT devices was analyzed, and the switching characteristics of IGBT devices were measured under maximum DC-link voltage and entire current rating range at the temperatures of −40 °C, 25 °C, and 150 °C, respectively, finding that neither the excessive turn-off overvoltage of IGBTs nor the snappy reverse recovery of FWDs was observed.
Studies on Methane Gas Hydrate Formation Kinetics Enhanced by Isopentane and Sodium Dodecyl Sulfate Promoters for Seawater Desalination
Authors: Bamaga Omar , Ahmed Iqbal , Wafiyah Asim M. , Albeirutty Mohammed , Abulkhair Hani , Shaiban Amer , Linga Praveen
Published: December 2022

Methane hydrate applications in gas storage and desalination have attracted increasing attention in recent years. In the present work, the effect of isopentane (IP), sodium dodecyl sulfate (SDS), and IP/SDS blends as promoters on methane hydrate formation kinetics, in terms of the pressure–temperature (P-T) profile, gas uptake, hydrate induction time (HIT), and water-to-hydrate conversion ratio (WHCR), were studied for distilled water and seawater samples with an IP/water sample ratio of 3:10 (by volume) and an SDS/water sample ratio of 1:1000 (by mass). Each solution was tested in a stirred tank at 600 rpm at a temperature and pressure of 2 °C and 5.2–5.3 MPa. In the case of methane hydrate formation in distilled water, the highest WHCR attained was 9.97% without additives, and 45.71% and 72.28% for SDS and isopentane additives, respectively. However, when using seawater at a salinity of 3.9%, the highest WHCR attained was 2.26% without additives and 9.89% and 18.03% for SDS and IP promoters, respectively, indicating the inhibiting effect of salinity on hydrate formation. However, the HIT was longer for seawater hydrate formation, with an average of 13.1 min compared to 9.90 min for methane hydrate formation. Isopentane enhances the HIT for methane hydrate formation in seawater by 2.23 times compared to SDS. For methane hydrate formation in seawater, the presence of IP shortened the HIT by 15.6 min compared to the seawater sample without promoters. Additionally, a synergistic effect was observed when IP and SDS were combined and used in methane hydrate formation in distilled water and seawater systems. The positive effect of IP on methane hydrate formation is possibly due to the binary hydrate formation mechanism, which improves the hydrate formation thermodynamic and kinetic parameters.
Framework of Transactive Energy Market Strategies for Lucrative Peer-to-Peer Energy Transactions
Authors: Loganathan Arun S. , Ramachandran Vijayapriya , Perumal Angalaeswari Sendraya , Dhanasekaran Seshathiri , Lakshmaiya Natrayan , Paramasivam Prabhu
Published: December 2022

Leading to the enhancement of smart grid implementation, the peer-to-peer (P2P) energy transaction concept has grown dramatically in recent years allowing the end-users to successfully exchange their excess generation and demand in a more profitable way. This paper presents local energy market (LEM) architecture with various market strategies for P2P energy trading among a set of end-users (consumers and prosumers) in a smart residential locality. In a P2P fashion, prosumers/consumers can export/import the available generation/demand in the LEM at a profit relative to utility prices. A common portal known as the transactive energy market operator (TEMO) is introduced to manage the trading in the LEM. The goal of the TEMO is to develop a transaction agreement among P2P players by establishing a price for each transaction based on the price and trading demand provided by the participants. A few case studies on a location with ten residential P2P participants validate the performance of the proposed TEMO.
Numerical Simulation of an Improved Updraft Biomass Gasifier Based on Aspen Plus
Authors: Zhu Fugang , Shen Laihong , Xu Pengcheng , Yuan Haoran , Hu Ming , Qi Jingwei , Chen Yong
Published: December 2022

In this paper, numerical investigation and optimization is conducted upon an improved updraft gasifier which is expected to overcome the weakness of conventional updraft gasifier. The comprehensive Aspen Plus model of the improved updraft gasifier is based on the RYield and RCSTR reactor. The tar prediction model is constructed, and the yield of tar is determined by the volatile of biomass and gasification temperature. The Aspen Plus simulation results agree very well with experiment results for the product yields and gasification efficiency, which shows the accuracy of the Aspen Plus model. The tar content in syngas of the improved gasifier is proved to be much lower than that of the conventional one by this model. The inflection point of the gasification efficiency occurs when air ratio is 0.25, and the optimum steam proportion in the air is 7.5%. Such a comprehensive investigation could provide necessary information for the optimal design and operation of the improved updraft gasifier.
Trajectories of Job Burnout among Bus Drivers in China: A Three-Year Follow-Up Study
Authors: Huang Andi , Liu Lili , Wang Xiayong , Li Xueguo , Li Jiahong , Luo Cong , Chen Jianbin , Zhao Jingbo
Published: December 2022

This study aimed to characterize job burnout in longitudinal trajectories among bus drivers and examine the impact of variables related to job burnout for trajectories. A longitudinal study was conducted in 12,793 bus drivers in Guangdong province, China, at 3-year follow-up assessments. Growth mixture modeling (GMM) was used to estimate latent classes of burnout trajectories and multinomial logistic regression models were applied to predict membership in the trajectory classes. In general, there was a decrease in job burnout in 3 years [slope = −0.29, 95%CI = (−0.32, −0.27)]. Among those sub-dimensions, reduced personal accomplishment accounted for the largest proportion. GMM analysis identified five trajectory groups: (1) moderate-decreased (n = 2870, 23%), (2) low-stable (n = 5062, 39%), (3) rapid-decreased (n = 141, 1%), (4) moderate-increased (n = 1504, 12%), and (5) high-stable (n = 3216, 25%). Multinomial logistic regression estimates showed that depression symptoms, anxiety symptoms, and insomnia were significant negative predictors, while daily physical exercise was a significantly positive predictor. We found an overall downward trend in bus drivers’ burnout, particularly in the sub-dimension of personal accomplishment. Mentally healthier drivers and those who were usually exercising were more resilient to occupational stress and less likely to suffer burnout.
Calcium Enhances Thallium Uptake in Green Cabbage (Brassica oleracea var. capitata L.)
Authors: Jia Yanlong , Xiao Tangfu , Sun Jialong , Ning Zengping , Xiao Enzong , Lan Xiaolong , Chen Yuxiao
Published: December 2022

Thallium (Tl) is a nonessential and toxic trace metal that is detrimental to plants, but it can be highly up-taken in green cabbage (Brassica oleracea L. var. capitata L.). It has been proven that there is a significant positive correlation between Tl and Calcium (Ca) contents in plants. However, whether Ca presents a similar role for alleviating Tl toxicity in plants remains unclear, and little is known in terms of evidence for both Ca-enhanced uptake of Tl from soils to green cabbage and associated geochemical processes. In this study, we investigated the influence of Ca in soils on Tl uptake in green cabbage and the associated geochemical process. The pot experiments were conducted in 12 mg/kg Tl(I) and 8 mg/kg Tl(III) treatments with various Ca dosages. The results showed that Ca in soils could significantly enhance Tl uptake in green cabbage, increasing 210% in content over the control group. The soluble concentrations of Tl were largely increased by 210% and 150%, respectively, in 3.0 g/kg Ca treatment, compared with the corresponding treatment without Ca addition. This was attributed to the geochemical process in which the enhanced soluble Ca probably replaces Tl held on the soil particles, releasing more soluble Tl into the soil solution. More interestingly, the bioconcentration factor of the leaves and whole plant for the 2.0, 2.5, 3.0 g/kg Ca dosage group were greatly higher than for the non-Ca treatment, which could reach 207%, implying the addition of Ca can improve the ability of green cabbage to transfer Tl from the stems to the leaves. Furthermore, the pH values dropped with the increasing Ca concentration treatment, and the lower pH in soils also increased Tl mobilization, which resulted in Tl accumulation in green cabbage. Therefore, this work not only informs the improvement of agricultural safety management practices for the farming of crops in Tl-polluted and high-Ca-content areas, but also provides technical support for the exploitation of Ca-assisted phytoextraction technology.
CRISPR/Cas9-Mediated Gene Editing in Salmonids Cells and Efficient Establishment of Edited Clonal Cell Lines
Authors: Strømsnes Trygve A. H. , Schmidke Sebastian E. , Azad Mitra , Singstad Øyvind , Grønsberg Idun M. , Dalmo Roy A. , Okoli Arinze S.
Published: December 2022

Finfish production has seen over three-fold increase in the past 30 years (1990–2020), and Atlantic salmon (A. salmon; salmo salar) accounted for approximately 32.6% of the total marine and coastal aquaculture of all finfish species in the year 2020, making it one of the most profitable farmed fish species globally. This growth in production is, however, threatened by a number of problems which can be solved using the CRISPR/Cas technology. In vitro applications of CRISPR/Cas using cell lines can complement its in vivo applications, but salmonids-derived cell lines are difficult to gene edit because they grow slowly, are difficult to transfect and isolate single clones of gene-edited cells. While clonal isolation of the gene-edited Chinook salmon cell line (CHSE-214) has successfully been performed, there is no report of successful clonal isolation of the gene-edited A. salmon ASK-1 and SHK-1cell lines. In the current study, two gene loci—cr2 and mmp9 of A. salmon—were efficiently edited using the ribonucleoprotein (RNP) and plasmid CRISPR/Cas9 strategies. Edited cells were enriched using flow cytometer-activated cell sorting (FACS), followed by clonal isolation and expansion of edited cells. The study both confirms the recent report of the highly efficient editing of these widely used model cell lines, as well as extends the frontline in the single-cell cloning of gene-edited salmonids cells. The report also highlights the pitfalls and future directions in the application of CRISPR/Cas9 in these cells.
Dysregulated Expression of Transposable Elements in TDP-43M337V Human Motor Neurons That Recapitulate Amyotrophic Lateral Sclerosis In Vitro
Authors: Valdebenito-Maturana Braulio , Rojas-Tapia Matias Ignacio , Carrasco Mónica , Tapia Juan Carlos
Published: December 2022

Amyotrophic lateral sclerosis (ALS) is a disease that progressively annihilates spinal cord motor neurons, causing severe motor decline and death. The disease is divided into familial and sporadic ALS. Mutations in the TAR DNA binding protein 43 (TDP-43) have been involved in the pathological emergence and progression of ALS, although the molecular mechanisms eliciting the disease are unknown. Transposable elements (TEs) and DNA sequences capable of transposing within the genome become dysregulated and transcribed in the presence of TDP-43 mutations. We performed RNA-Seq in human motor neurons (iMNs) derived from induced pluripotent stem cells (iPSCs) from TDP-43 wild-type—iMNs-TDP-43WT—and mutant—iMNs-TDP-43M337V—genotypes at 7 and 14 DIV, and, with state-of-the-art bioinformatic tools, analyzed whether TDP-43M337V alters both gene expression and TE activity. Our results show that TDP-43M337V induced global changes in the gene expression and TEs levels at all in vitro stages studied. Interestingly, many genetic pathways overlapped with that of the TEs activity, suggesting that TEs control the expression of several genes. TEs correlated with genes that played key roles in the extracellular matrix and RNA processing: all the regulatory pathways affected in ALS. Thus, the loss of TE regulation is present in TDP-43 mutations and is a critical determinant of the disease in human motor neurons. Overall, our results support the evidence that indicates TEs are critical regulatory sequences contributing to ALS neurodegeneration.
Cardiac Sarcomere Signaling in Health and Disease
Authors: Martin Ashley A. , Thompson Brian R. , Hahn Dongwoo , Angulski Addeli Bez Batti , Hosny Nora , Cohen Houda , Metzger Joseph M.
Published: December 2022

The cardiac sarcomere is a triumph of biological evolution wherein myriad contractile and regulatory proteins assemble into a quasi-crystalline lattice to serve as the central point upon which cardiac muscle contraction occurs. This review focuses on the many signaling components and mechanisms of regulation that impact cardiac sarcomere function. We highlight the roles of the thick and thin filament, both as necessary structural and regulatory building blocks of the sarcomere as well as targets of functionally impactful modifications. Currently, a new focus emerging in the field is inter-myofilament signaling, and we discuss here the important mediators of this mechanism, including myosin-binding protein C and titin. As the understanding of sarcomere signaling advances, so do the methods with which it is studied. This is reviewed here through discussion of recent live muscle systems in which the sarcomere can be studied under intact, physiologically relevant conditions.
Acting on the CFTR Membrane-Spanning Domains Interface Rescues Some Misfolded Mutants
Authors: Baatallah Nesrine , Elbahnsi Ahmad , Chevalier Benoit , Castanier Solène , Mornon Jean-Paul , Pranke Iwona , Edelman Aleksander , Sermet-Gaudelus Isabelle , Callebaut Isabelle , Hinzpeter Alexandre
Published: December 2022

ABC transporters are large membrane proteins sharing a complex architecture, which comprises two nucleotide-binding domains (NBDs) and two membrane-spanning domains (MSDs). These domains are susceptible to mutations affecting their folding and assembly. In the CFTR (ABCC7) protein, a groove has been highlighted in the MSD1 at the level of the membrane inner leaflet, containing both multiple mutations affecting folding and a binding site for pharmaco-chaperones that stabilize this region. This groove is also present in ABCB proteins, however it is covered by a short elbow helix, while in ABCC proteins it remains unprotected, due to a lower position of the elbow helix in the presence of the ABCC-specific lasso motif. Here, we identified a MSD1 second-site mutation located in the vicinity of the CFTR MSD1 groove that partially rescued the folding defect of cystic fibrosis causing mutations located within MSD1, while having no effect on the most frequent mutation, F508del, located within NBD1. A model of the mutated protein 3D structure suggests additional interaction between MSD1 and MSD2, strengthening the assembly at the level of the MSD intracellular loops. Altogether, these results provide insightful information in understanding key features of the folding and function of the CFTR protein in particular, and more generally, of type IV ABC transporters.
The IL-1 Family and Its Role in Atherosclerosis
Authors: González Leticia , Rivera Katherine , Andia Marcelo E. , Martínez Rodriguez Gonzalo
Published: December 2022

The IL-1 superfamily of cytokines is a central regulator of immunity and inflammation. The family is composed of 11 cytokines (with agonist, antagonist, and anti-inflammatory properties) and 10 receptors, all tightly regulated through decoy receptor, receptor antagonists, and signaling inhibitors. Inflammation not only is an important physiological response against infection and injury but also plays a central role in atherosclerosis development. Several clinical association studies along with experimental studies have implicated the IL-1 superfamily of cytokines and its receptors in the pathogenesis of cardiovascular disease. Here, we summarize the key features of the IL-1 family, its role in immunity and disease, and how it helps shape the development of atherosclerosis.
Recent Developments in Zn-Based Biodegradable Materials for Biomedical Applications
Authors: Hussain Muzamil , Ullah Sami , Raza Muhammad Rafi , Abbas Naseem , Ali Ahsan
Published: December 2022

Zn-based biodegradable alloys or composites have the potential to be developed to next-generation orthopedic implants as alternatives to conventional implants to avoid revision surgeries and to reduce biocompatibility issues. This review summarizes the current research status on Zn-based biodegradable materials. The biological function of Zn, design criteria for orthopedic implants, and corrosion behavior of biodegradable materials are briefly discussed. The performance of many novel zinc-based biodegradable materials is evaluated in terms of biodegradation, biocompatibility, and mechanical properties. Zn-based materials perform a significant role in bone metabolism and the growth of new cells and show medium degradation without the release of excessive hydrogen. The addition of alloying elements such as Mg, Zr, Mn, Ca, and Li into pure Zn enhances the mechanical properties of Zn alloys. Grain refinement by the application of post-processing techniques is effective for the development of many suitable Zn-based biodegradable materials.
Effect of Al Addition on Microstructure and Properties of CoCrNi Medium-Entropy Alloy Prepared by Powder Metallurgy
Authors: Ding Xuekun , He Jichang , Zhong Jinde , Wang Xiang , Li Zhanjiang , Tian Jun , Dai Pinqiang
Published: December 2022

Powder metallurgy possesses the advantages of low energy consumption, less material consumption, uniform composition, and near-final forming. In order to improve the mechanical properties and high-temperature oxidation resistance of CoCrNi medium-entropy alloy (MEA), CoCrNiAlX (X = 0, 0.1, 0.3, 0.5, 0.7) MEAs were prepared using mechanical alloying (MA) and spark-plasma sintering (SPS). The effect of aluminum content on the microstructure and properties of the MEAs was investigated. The results show that the CoCrNi MEA is composed of face center cubic (fcc) phase and some carbides (Cr23C6). With the increase in Al content, there exists Al2O3 precipitation. When the Al content is increased to Al0.5 and Al0.7, the body center cubic (bcc) phase begins to precipitate. The addition of aluminum significantly enhances the properties of the alloys, especially those containing fcc+bcc dual-phase solid solutions. The yield strength, compressive strength, and hardness of CoCrNiAl0.7 alloy are as high as 2083 MPa, 2498 MPa, and 646 HV, respectively. The high-temperature resistance also reaches the oxidation resistance level. Different oxides include Cr2O3, Al2O3, and (Co, Ni) Cr2O4 and NiCrO3 spinel oxides formed on the surface of alloys. The formation of an Al2O3 oxidation film prevents the further erosion of the matrix by oxygen elements.
Notch Effects on the Stress Intensity Factor and on the Fatigue Crack Path for Eccentric Circular Internal Cracks in Elliptically Notched Round Bars under Tensile Loading
Authors: Toribio Jesús , González Beatriz , Matos Juan-Carlos , González Iván
Published: December 2022

In this paper, stress intensity factor (SIF) solutions are numerically obtained for notched bars subjected to tensile loading containing an eccentric circular inner crack located in the cross-section corresponding to the notch root. The finite element method and the J-integral have been used to obtain the SIF and to analyze the effect on it of three elliptical notch geometries (of equal radial depth). The results show how the SIF is greater in the notched bars than in the smooth bar and within the former when the axial semi-axis of the notch rises, its effect being greater as the diameter and eccentricity of the inner crack increase. In addition, the fatigue growth of an eccentric crack induces an increase in such eccentricity, greater as the notch axial semi-axis increases. The cause of these phenomena can be attributed to the constraint loss caused by the notch, which also facilitates bending of the specimen due to the asymmetry generated by the crack eccentricity.
Effect of Cooling Rate and Sulfur Content on Sulfide Inclusions in Invar Alloy
Authors: Chen Jing , Feng Yanbiao , Zheng Shaobo
Published: December 2022

Invar alloy has been widely used in modern industry for its extremely low coefficients of thermal expansion (CTE). Sulfide inclusions have a significant influence on the mechanical performance and corrosion resistance of Invar alloy. To improve the mechanical properties of this alloy, which are significantly influenced by the existing sulfide inclusions, a good understanding of the characteristics, including the morphology, size, distribution, and formation mechanism of sulfide inclusions, is required. This study outlines three different cooling modes, water cooling (43.1 K/s), air cooling (16.8 K/s), and furnace cooling (3.1 K/s), to examine the characteristics of calcium sulfide (CaS) inclusions. In addition, a variety of initial sulfur contents under air cooling conditions were investigated. Both laboratory experiments and thermodynamic calculations support the research foundation. The sulfide inclusion particles were extracted through non-aqueous solution electrolysis for further morphology analysis. From the results, we conclude that the cooling rate affects the sulfide inclusion size through the local solidification time. The faster the cooling rate, the larger the average inclusion size. The cooling rate also indirectly influences the morphology of calcium sulfide inclusions that precipitated during the solidification process. In addition, the precipitation of CaS takes precedence over MnS under any circumstances. MnS can only precipitate when there is excessive S content. The different initial S content derived separate supersaturation during CaS precipitation and growth, further influencing the inclusion morphologies.
A Metallurgical Dynamics-Based Method for Production State Characterization and End-Point Time Prediction of Basic Oxygen Furnace Steelmaking
Authors: Qian Qingting , Dong Qianqian , Xu Jinwu , Zhao Wei , Li Min
Published: December 2022

Basic Oxygen Furnace (BOF) steelmaking is an important way for steel production. Correctly recognizing different blowing periods and abnormal refining states is significant to ensure normal production process, while accurately predicting the end-point time helps to increase the first-time qualification rate of molten steel. Since the decarburization products CO and CO2 are the main compositions of off-gas, information of off-gas is explored for BOF steelmaking control. However, the problem is that most of the existing research directly gave the proportions of CO and CO2 as model input but barely considered the variation information of off-gas to describe the production state. At the same time, the off-gas information can be expected to recognize the last blowing period and predict the end-point time earlier than the existing methods that are based on sub-lance or furnace flame image, but little literature makes an attempt. Therefore, this work proposes a new method based on functional data analysis (FDA) and phase plane (PP), defined as FDA-PP, to describe and predict the BOF steelmaking process from the metallurgical dynamics viewpoint. This method extracts the total proportion of CO and CO2 and its first-order derivative as dynamics features of steelmaking process via FDA, which indicate the reaction velocity and acceleration of decarburization reaction, and describes the evolution of dynamics features via PP. Then, the FDA-PP method extracts the features of phase trajectories for production state recognition and end-point time prediction. Experiments on a real production dataset demonstrate that the FDA-PP method has higher production state recognition accuracy than the classical phase space, SVM, and BP methods, which is 87.78% for blowing periods of normal batches, 90.94% for splashing anomaly, and 81.29% for drying anomaly, respectively. At the same time, the FDA-PP method decreases the mean relative prediction error (MRE) of the end-point time prediction for abnormal batches by about 10% compared with the SVM and BP methods.