bluuebunny/crossref_metadata_2025 huggingface dataset:
Loop-Mediated Isothermal Amplification Assay for Detecting Tumor Markers and Human Papillomavirus: Accuracy and Supplemental Diagnostic Value to Endovaginal MRI in Cervical Cancer
Authors: Wormald Benjamin , Rodriguez-Manzano Jesus , Moser Nicolas , Pennisi Ivana , Ind Thomas E. J. , Vroobel Katherine , Attygalle Ayoma , Georgiou Pantelis , deSouza Nandita M.
Published: November 2021

Objective

To establish the sensitivity and specificity of a human papillomavirus (HPV) and tumor marker DNA/mRNA assay for detecting cervical cancer that is transferrable to a Lab-on-a-chip platform and determine its diagnostic benefit in early stage disease when used in conjunction with high-resolution endovaginal magnetic resonance imaging (MRI).

Methods

Forty-one patients (27 with Stage1 cervical cancer [Group1] and 14 non-cancer HPV negative controls [Group2]) had DNA and RNA extracted from cervical cytology swab samples. HPV16, HPV18, hTERT, TERC/GAPDH and MYC/GAPDH concentration was established using a loop mediated isothermal amplification (LAMP) assay. Thresholds for tumor marker detection for Group1 were set from Group2 analysis (any hTERT, TERC/GAPDH 3.12, MYC/GAPDH 0.155). Group 1 participants underwent endovaginal MRI. Sensitivity and specificity for cancer detection by LAMP and MRI individually and combined was documented by comparison to pathology.

Results

Sensitivity and specificity for cancer detection was 68.8% and 77.8% if any tumor marker was positive regardless of HPV status (scenario1), and 93.8% and 55.8% if tumor marker or HPV were positive (scenario 2). Adding endovaginal MRI improved specificity to 88.9% in scenario 1 (sensitivity 68.8%) and to 77.8%% in scenario2 (sensitivity 93.8%).

Conclusion

Specificity for cervical cancer detection using a LAMP assay is superior with tumor markers; low sensitivity is improved by HPV detection. Accuracy for early stage cervical cancer detection is optimal using a spatially multiplexed tumor marker/HPV LAMP assay together with endovaginal MRI.
A Comprehensive Strategy to Pain Management for Cancer Patients in an Inpatient Rehabilitation Facility
Authors: Pugh Terrence M. , Squarize Fabiana , Kiser Allison L.
Published: November 2021

Cancer pain has been shown to have a significant negative impact on health-related quality of life (HRQoL) for people experiencing it. This is also true for patients admitted to inpatient rehabilitation facilities (IRFs). An interdisciplinary approach is often needed to fully address a person's pain to help them attain maximum functional independence and to ensure a safe discharge home. Improving a patient's performance status in an IRF may also be a crucial determinant in their ability to continue receiving treatment for their cancer. However, if a person is determined to no longer be a candidate for aggressive, disease modulating treatment, IRFs can also be utilized to help patients and family's transition to comfort directed care with palliative or hospice services. This article will discuss the interventions of the multidisciplinary inpatient rehabilitation team to address a person's pain.
Drug-Repositioning Approaches Based on Medical and Life Science Databases
Authors: Zamami Yoshito , Hamano Hirofumi , Niimura Takahiro , Aizawa Fuka , Yagi Kenta , Goda Mitsuhiro , Izawa-Ishizawa Yuki , Ishizawa Keisuke
Published: November 2021

Drug repositioning is a drug discovery strategy in which an existing drug is utilized as a therapeutic agent for a different disease. As information regarding the safety, pharmacokinetics, and formulation of existing drugs is already available, the cost and time required for drug development is reduced. Conventional drug repositioning has been dominated by a method involving the search for candidate drugs that act on the target molecules of an organism in a diseased state through basic research. However, recently, information hosted on medical information and life science databases have been used in translational research to bridge the gap between basic research in drug repositioning and clinical application. Here, we review an example of drug repositioning wherein candidate drugs were found and their mechanisms of action against a novel therapeutic target were identified via a basic research method that combines the findings retrieved from various medical and life science databases.
Narcissistic Traits and Executive Functions
Authors: Nenadić Igor
Published: November 2021

Several personality disorders have been associated with cognitive impairment, including executive functions like working memory. Yet, it is unclear whether subclinical expression in non-clinical persons is associated with cognitive functioning. Recent studies indicate that non-clinical subjects might, in fact, perform better with increasing moderate to mild expressions of narcissistic features. We tested working memory performance in a cohort ofn=70 psychiatrically and neurologically healthy subjects using Wechsler Adult Intelligence Scale (WAIS/WIE) subtests Arithmetic, Digit Span and Letter-Number Sequencing, and assessed narcissistic features using three different inventories: the widely used Narcissistic Personality Inventory (NPI), as well as two clinically used measures of narcissistic traits and states, respectively, derived from schema-focused therapy, i.e., the Young Schema Questionnaire (YSQ) entitlement/grandiosity subscale and the Schema Mode Inventory (SMI) self-aggrandizer subscale. In accordance with our hypothesis, we found nominally significant positive correlations of WIE Arithmetic performance with NPI total score (Spearman’s rho=0.208;p=0.043) and SMI self-aggrandizer scale (Spearman’s rho=0.231;p=0.027), but findings did not survive false discovery rate (FDR) adjustment for multiple comparisons (pFDR=0.189 and pFDR=0.243, respectively). While our findings add to recent studies on cognitive performance in subclinical narcissism, they fail to demonstrate an association of cognitive performance with narcissistic traits across multiple working memory tests, indicating the need for additional study, including complementary executive functions in larger cohorts and ranges of phenotype expression.
Decision-Making Model for Addressing Role Conflict for Psychology Trainees When Supporting Family and Community
Authors: Larez Natalie A. , Sharkey Jill D.
Published: November 2021

As the field of psychology continues to make efforts to diversify the field, training programs must adapt to include the needs of diverse students. Universities in the United States mirror middle-class norms and values, which implies that students are expected to separate from familial roles and focus on their personal growth. This conflicts with core values and intentions of students from collectivist cultures. Although psychology trainees are obligated to adhere to professional ethical standards, a growing number of psychology trainees from collectivistic cultures need support to manage role conflict within potentially ambiguous standards regarding how to care for family and community members. This need is further complicated when training programs consider the lack of equitable access to mental health care resources in communities where their psychology trainees come from. In this paper, we engage in ethical decision making to address two scenarios representing role conflict between training program expectations and collectivist community and familial obligations. Through this exercise we develop and propose a Decision-Making Model for Addressing Role Conflict for Psychology Trainees. This conceptual model details a novel framework to assist psychology trainees when addressing the mental health of family and community while also providing guidance to help graduate training programs proactively equip their students with the skills and ethical framework they need to balance role conflicts such as when family and community members desire and need mental health support.
Merging the Social Influence Theory and the Goal-Framing Theory to Understand Consumers’ Green Purchasing Behavior: Does the Level of Sensitivity to Climate Change Really Matter?
Authors: Yang Xianchuan , Tseng Yafen , Lee Beyfen
Published: November 2021

This study explored the formation of consumers’ green purchasing behavior (GPB) and investigated the moderating effect of sensitivity to climate change (SCC) to address this current knowledge gap. An integrated model merging the Social Influence Theory and the Goal-framing Theory was developed with the Stimulus-Organism-Response (S-O-R) paradigm. An empirical study was conducted, surveying 583 respondents and analyzing the questionnaire results using structural equation modeling. The results show that media, family, and peer influence (PEI) can effectively activate the consumers’ goal frames. Hedonic and normative goals had significant positive influences on GPB, while gain goals had no significant effect. SCC was found to significantly moderate social influence on GPB through the consumers’ goal frames. This research provided strong empirical support on understanding the relationship between social influence and GPB through three goal frames. In addition, the potential differences of the GPB formation process in two subgroups (high SCC and low SCC) are also investigated. The results of this study can help green practitioners develop more effective marketing strategies and incentives targeted to consumers with varying levels of environmental consciousness or sensitivity.
Relationship Between Gaming Disorder, Self-Compensation Motivation, Game Flow, Time Spent Gaming, and Fear of Missing Out Among a Sample of Chinese University Students: A Network Analysis
Authors: Li Li , Niu Zhimin , Griffiths Mark D. , Mei Songli
Published: November 2021

Background and Aims:In previous correlational research, the relationship between gaming disorder (GD), compensation motivation, game flow, time spent gaming, and fear of missing out (FoMO) has been examined. However, network analysis has rarely been applied to explore the relationship between GD, self-compensation motivation, game flow, time spent gaming, and FoMO. Therefore, the present study used network analysis to examine the relationship between the aforementioned variables among a sample of gamers.

Methods:The present study comprised gamers (N= 1,635) recruited from three Chinese universities, who completed an online survey including the Gaming Disorder Test, Self-Compensation Motivation Questionnaire, Game Flow Questionnaire, and Trait-State Fear of Missing Out Scale, as well as four items related to time spent gaming.

Results:Self-compensation motivation, game flow, time spent gaming, and FoMO were all significantly and positively associated with GD. In the domain-level and facet-level networks, weekday gaming hours and weekend gaming hours had the strongest edge intensity. The domain-level, facet-level, and item-level networks analysis also showed that GD was connected with self-compensation motivation, game flow, time spent gaming, and FoMO. The network structure demonstrated a significant difference between males and females (2.33 vs. 2.81,p= 0.001) using the domain-level network comparison test (NCT).

Conclusions:The results suggest that GD is closely associated with self-compensation motivation, game flow, time spent gaming, and FoMO. FoMO and gaming motivation (i.e., self-compensation and game flow) may increase time spent gaming and facilitate GD. Therefore, interventions that decrease game immersion and time spent gaming are likely to decrease GD.
Every Dog Has Its Data: Evaluation of a Technology-Aided Canine Rabies Vaccination Campaign to Implement a Microplanning Approach
Authors: Monroe Benjamin , Ludder Fleurinord , Dilius Pierre , Crowdis Kelly , Lohr Frederic , Cleaton Julie , Gamble Luke , Blanton Jesse , Etheart Melissa , Pieracci Emily G. , Natal Vigilato Marco Antonio , Molina-Flores Baldomero , Millien Max , Gibson Andrew D. , Wallace Ryan M.
Published: November 2021

Background: Robust dog vaccination coverage is the primary way to eliminate canine rabies. Haiti conducts annual canine mass vaccination campaigns, but still has the most human deaths in the Latin American and Caribbean region. We conducted an evaluation of dog vaccination methods in Haiti to determine if more intensive, data-driven vaccination methods, using smartphones for data reporting and geo-communication, could increase vaccination coverage to a level capable of disrupting rabies virus transmission.

Methods: Two cities were designated into “Traditional” and “Technology-aided” vaccination areas. Traditional areas utilized historical methods of vaccination staff management, whereas Technology-aided areas used smartphone-supported spatial coordination and management of vaccination teams. Smartphones enabled real time two-way geo-communication between campaign managers and vaccinators. Campaign managers provided geographic instruction to vaccinators by assigning mapped daily vaccination boundaries displayed on phone handsets, whilst vaccinators uploaded spatial data of dogs vaccinated for review by the campaign manager to inform assignment of subsequent vaccination zones. The methods were evaluated for vaccination effort, coverage, and cost.

Results: A total of 11,420 dogs were vaccinated during the 14-day campaign. The technology-aided approach achieved 80% estimated vaccination coverage as compared to 44% in traditional areas. Daily vaccination rate was higher in Traditional areas (41.7 vaccinations per team-day) compared to in technology-aided areas (26.8) but resulted in significantly lower vaccination coverages. The cost per dog vaccinated increased exponentially with the associated vaccination coverage, with a cost of $1.86 to achieve 25%, $2.51 for 50% coverage, and $3.19 for 70% coverage.

Conclusions: Traditional vaccination methods failed to achieve sufficiently high vaccination coverages needed to interrupt sustained rabies virus transmission, whilst the technology-aided approach increased coverage above this critical threshold. Over successive campaigns, this difference is likely to represent the success or failure of the intervention in eliminating the rabies virus. Technology-aided vaccination should be considered in resource limited settings where rabies has not been controlled by Traditional vaccination methods. The use of technology to direct health care workers based on near-real-time spatial data from the field has myriad potential applications in other vaccination and public health initiatives.
Unhealthy Immigrants: Sources for Health Gaps Between Immigrants and Natives in Israel
Authors: Semyonov-Tal Keren , Maskileyson Dina
Published: November 2021

The study focuses on sources for health gaps between Jewish immigrants and native-born Israelis. Unlike traditional immigrant societies where immigration is viewed as economically motivated, immigrants returning to Israel are viewed as the “returning diaspora”. Because immigrants in Israel are entitled to the same health benefits and medical services as native-born, we expect Israel to attract unhealthy immigrants in disproportionate numbers. The data for the analysis are obtained from the Israeli National Health Interview Survey (2013–2015). The data set provides detailed information on health status and illness, sociodemographic attributes and origin of immigrants. Three major origin groups of immigrants are distinguished: the former Soviet Union, Western Europeans or the Americans (mostly Ashkenazim), and Asians or North Africans (mostly Sephardim). Our findings lend support to the expectations that the health status of all immigrant groups is poorer than that of native-born Israelis. The nativity–illness gap is most pronounced in the case of male immigrants (from Europe or the Americas or South Africa or Australia) and for female immigrants (from countries in the Middle East or North Africa) and least pronounced in the case of immigrants arriving from the former Soviet Union for both gender groups. Decomposition of the gaps into components reveals that some portion of the illness gap can be attributed to nativity status, but the largest portion of the gap is attributed to demographic characteristics. Neither socioeconomic status nor health-related behavior accounts for a substantial portion of the nativity–illness gap for all subgroups of immigrants.
Intervención Cognitivo-Conductual en un Caso de Ansiedad Social
Authors: Reguera Lozano Santiago José
Published: November 2021

La ansiedad social es un trastorno caracterizado por el miedo intenso a situaciones sociales en las que la persona está expuesta a una posible evaluación por parte de otros individuos, por ejemplo, hablar en público. Presentamos el caso de un estudiante universitario de 21 años, que acude a consulta refiriendo problemas de interacción social e inseguridad. Esta conducta se mantiene por evitación de las situaciones sociales principalmente con desconocidos, que fomentan la inseguridad en sí mismo. Presenta pensamientos negativos automáticos evaluativos y síntomas fisiológicos propios de la ansiedad. Afronta las situaciones con gran dificultad, con presencia de rumiaciones y autovaloración negativa. El objetivo de la intervención, desde una perspectiva cognitivo-conductual, pretende optimizar sus interacciones sociales disminuyendo la ansiedad social, aumentar la seguridad en sí mismo y mejorar las habilidades sociales del paciente y su asertividad. Para la evaluación se han utilizado, además de una entrevista semiestructurada, los siguientes instrumentos: SCL-90, STAI, CORE-OM y NEO-FII. La propuesta de intervención incluyó técnicas de psicoeducación sobre la ansiedad social, reestructuración cognitiva para los pensamientos automáticos de evaluación negativa, ensayo de conducta y exposición en vivo a situaciones sociales y entrenamiento en habilidades sociales.  Los resultados obtenidos en el pre y post tratamiento muestran una disminución de la ansiedad tanto estado como rasgo, reducción de sintomatología clínica y aumento del bienestar y funcionamiento general. Tras la terapia se produce una disminución de factores relacionados con el malestar y la ansiedad social, además de un incremento de las relaciones sociales y aumento de autoconfianza.
Psicoterapia centrada en la persona y la ideación suicida
Authors: López Marín Alejandro
Published: November 2021

El presente articulo corresponde a un ensayo teórico que explora el trabajo centrado en la persona con clientes que presentan ideación suicida. Inicialmente se presenta un panorama general sobre el suicido, luego, se plantea una mirada comprensiva sobre el fenómeno de la ideación suicida desde el enfoque centrado en la persona, y, finalmente, se plantean algunas reflexiones sobre  el  proceso  psicoterapéutico  para  trabajar  con personas que presentan ideación suicida. Se concluye que este enfoque puede contribuir al trabajo terapéutico en personas que presentan ideación suicida, y, por lo mismo, la necesidad de realizar mayores investigaciones sobre el tema.
Adherencia y satisfacción en psicoterapia de grupo online vs presencial en tiempos de COVID-19
Authors: Castrillo Ramírez Paula , Mateos Agut Manuel
Published: November 2021

En las últimas décadas estamos presenciando un importante crecimiento de experiencias clínicas basadas en el uso de la tecnología para el desarrollo de la psicoterapia, nos encontramos en un periodo de investigación y desarrollo teórico ante la incorporación de un recurso emergente y su integración en la práctica profesional. Nuestro trabajo parte de una búsqueda bibliográfica acerca de la psicoterapia de grupo online a través de las publicaciones más relevantes hasta la actualidad, realizamos una síntesis de información acerca de las ventajas e inconvenientes, resultados sobre su eficacia. Además, aportamos un resumen de nuestra experiencia clínica en psicoterapia de grupo online por videoconferencia en el Hospital de Día del Servicio de Psiquiatría, analizando los resultados obtenidos sobre adherencia al tratemiento y satisfacción de los participantes, en su comparación con la psicoterapia de grupo presencial. En este estudio comparamos la asistencia en Hospital de Día durante los meses de mayo y junio de 2019 (antes de la pandemia COVID-19) en psicoterapia grupal presencial, con la asistencia en los mismos meses en 2020, en esta ocasión online. En este segundo grupo evaluamos la percepción subjetiva de satisfacción a través de un cuestionario ad hoc realizado por este grupo mediante fusión de cuestionarios previos. Observamos que disminuye la asistencia de manera significativa en el grupo virtual, no encontrando diferencias significativas en la comparación de asistencia por grupo diagnóstico; que la satisfacción con la psicoterapia online es aceptable; y observamos el desarrollo de fenómenos propios de la relación grupal en su formato virtual.
Psicoanálisis Relacional y Psicoterapia Individual Sistémica: Epistemología y Psicopatología.
Authors: De Pablo Urban Juan Miguel
Published: November 2021

En el presente artículo se analizan algunos de los elementos teóricos comunes encontrados entre el psicoanálisis relacional y la psicoterapia individual sistémica, en particular, aquellos que hacen referencia a la importancia del modelo epistemológico aportado desde la posmodernidad, a la concepción que prima sobre el concepto de enfermedad y/o la salud mental y, en consecuencia, a la visión existente en torno al uso del diagnóstico psicopatológico en psicoterapia.
Profile of the Small-Scale Farms Willing to Cooperate—Evidence from Lithuania
Authors: Droždz Jolanta , Vitunskienė Vlada , Novickytė Lina
Published: November 2021

Cooperatives cover a large part of the agricultural sectors and have substantial market shares in agri-food supply chains in the EU Western countries. They account for approximately half of agricultural trade in the EU. By contrast, in the EU Western countries, where farmer cooperatives are widespread and successful, agricultural cooperation in Lithuania has developed intermittently in the last century. We still have very limited knowledge of why the country’s agricultural producers (especially smallholder farmers) are reluctant to cooperate in Lithuania. The aim of this study is to assess the level of the willingness to cooperate among smallholder farmers in Lithuania and to draw up the profiles of small-scale farms that participate in and intend to join cooperatives and, conversely, that do not participate in cooperatives and do not intend to do so. To achieve this goal, a representative survey of small-scale farms was conducted. Results of surveys carried out in 2019 in Lithuania on a group of 1002 small-scale farms showed that only 8% of the surveyed farms participate in producer groups or cooperatives, while another 8% intend to participate. Small-scale farms in Lithuania have weak market integration, with no bargaining power on input and output markets. The vast majority of small-scale farms are reluctant to participate in cooperative activities in Lithuania. Therefore, this study aimed to determine the profile of a small farm that tends to cooperate. The main social characteristics of farm managers and economic factors of farms willing to cooperate have been identified.
Interaction of Inherited Microbiota from Cover Crops with Cash Crops
Authors: Ulcuango Kelly , Navas Mariela , Centurión Nelly , Ibañez Miguel Á. , Hontoria Chiquinquirá , Mariscal-Sancho Ignacio
Published: November 2021

Cover crops (CC) provide important ecosystem services that are demanded to achieve more sustainable agrosystems. However, the legacy effects of CC on the microbial community structure and its interactions with the subsequent cash crops (CaC) are still poorly understood, especially when CC mixtures are involved. In this work, five CC (3 monocultures and 2 mixtures) were selected in an experiment under semi-controlled conditions to investigate if CC monocultures and mixtures differed in their effects on soil and crop variables and if the identity of the subsequent crop modulates these effects. The two most consumed crops worldwide, wheat and maize, were sown separately after CC. The legacy effects of CC on the studied microbial variables largely depended on the interaction with the CaC. The vetch and the barley-vetch mixture stood out by providing the microbial conditions that enhanced the absorption of macro- and micronutrients, to finally seek the highest wheat biomass (>80% more than the control). In maize, the effects of CC on soil microbiota were more limited. The soil microbial responses for CC mixtures were complex and contrasting. In wheat, the barley-vetch mixture behaved like barley monoculture, whereas in maize, this mixture behaved like vetch monoculture. In both CaC, the barley-melilotus mixture differed completely from its monocultures, mainly through changes in archaea, Glomeromycota, and F:B ratio. Therefore, it is necessary to deepen the knowledge on the CC-CaC-microbial interactions to select the CC that most enhance the sustainability and yield of each agrosystem.
Exploring the Phenotypic Stability of Soybean Seed Compositions Using Multi-Trait Stability Index Approach
Authors: Abdelghany Ahmed M. , Zhang Shengrui , Azam Muhammad , Shaibu Abdulwahab S. , Feng Yue , Qi Jie , Li Jing , Li Yanfei , Tian Yu , Hong Huilong , Lamlom Sobhi F. , Li Bin , Sun Junming
Published: November 2021

In order to ensure an ongoing and long-term breeding progress of soybean, stable sources of major quality traits across multi-environments need to be identified. Here, a panel of 135 soybean genotypes was tested in three different Chinese environments, including Beijing, Anhui, and Hainan during the 2017 and 2018 growing seasons to identify stable genotypes for cultivation under varying environmental conditions. The weighted average of absolute scores biplot (WAASB) for the best linear unbiased predictions of the genotype-environment interaction and multi-trait stability index (MTSI) were utilized to determine the stability of the soybeans for seven seed composition traits viz; protein content, oil content, and five fatty acids (palmitic, stearic, oleic, linoleic, and linolenic acids). Based on the WAASB index, the following genotypes were identified as stable genotypes for some specific traits; ZDD12828 and ZDD12832 for protein content, WDD01583 and WDD03025 for oil content, ZDD23040 for palmitic acid, WDD00033 for stearic acid, ZDD23822 for oleic acid, ZDD11183 for linoleic acid, and ZDD08489 for linolenic acid. Furthermore, based on MTSI at a selection intensity of 10%, 14 soybean genotypes were selected for their average performance and stability. Overall, the MTSI was shown to be a powerful and simple tool for identifying superior genotypes in terms of both performance and stability, hence, identifying stable soybean genotypes for future breeding programs of quality traits.
Molecular Characterization of Spider Plant (Cleome gynandra) Accessions Using SSR Markers
Authors: Thovhogi Fhatuwani , Gwata Eastonce T. , Mchau Godwin R. A. , Safodien Sieyaam S. , Koopman Trevor
Published: November 2021

Spider plant (Cleome gynandra L.) is an indigenous leafy vegetable that is a rich source of minerals, proteins, vitamins, flavonoids, antioxidants and many other compounds. Morphological characterization of the germplasm for optimal genetic exploitation is limited by environmental factors. The objective of this study was to determine the degree of genetic diversity between exotic and local spider plant accessions using molecular markers. The spider plant germplasm consisted of 18 local and exotic accessions. Seven polymorphic simple sequence repeat (SSR) markers were used in the study. The SSR marker combinations revealed a total of 46 alleles at 7 loci, with an average of 6.57 alleles per locus. An average polymorphic information index of 0.69 was recorded. According to Nei’s genetic distance analysis, low population differentiation was observed between the local and exotic spider plant accessions, which was indicated by a low fixation index value of 0.024 and a high Nm (10.20). Analysis of molecular variance showed no significant (p < 0.327) variation among and within the local and exotic accessions. Furthermore, low population differentiation was recorded when accessions were grouped based on their stem colour, with an Fst value of 0.007. In both the hierarchical clustering using an unweighted pair group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCoA), the local accession ‘ML-3-KK’ was grouped alone, suggesting that this accession was different from the rest of the accessions and could be useful in selection programmes for spider plant genetic improvement. Both the UPGMA and PCoA were similar in clustering the eighteen spider plant accessions into four groups. The local spider plant accessions were more genetically diverse (with sixteen rare alleles) when compared to the exotic accessions (with only three rare alleles). The rare alleles provided useful information on the genetic variability in certain loci and identification of accessions that could be used as parental lines in spider plant breeding programmes.
Identification of Soybean Yield QTL in Irrigated and Rain-Fed Environments
Authors: Lee Geung-Joo , Lee Sungwoo , Carter Tommy E. , Shannon Grover , Boerma H. Roger
Published: November 2021

Drought is the primary abiotic stress that limits yield of soybean (Glycine max (L.) Merr.). The study aimed to identify yield-related quantitative trait loci (QTLs) in soybeans using a population of 160 F4-derived lines from ‘Hutcheson’ × PI 471938 crosses, which were cultivated under rain-fed and irrigated conditions. Seed yield was determined based on a total of nine irrigated and five rain-fed environments over two years. Twenty and twenty-seven SSR markers associated with yield (p ≤ 0.05) were identified in the irrigated and rain-fed environments, respectively. Four markers accounted for 22% of the yield variation in the irrigated environments (IR-YLD) and five markers explained 34% of the yield variation in the rain-fed environments (RF-YLD). Two independent IR-YLD and RF-YLD QTLs on chromosome (Chr) 13 (LG-F) were mapped to the Satt395-Sat_074 interval (4.2 cM) and near Sat_375 (3.0 cM), which explained 8% (LOD = 2.6) and 17% (LOD = 5.5) of the yield variation, respectively. The lines homozygous for the Hutcheson allele at the IR-YLD QTL linked to Sat_074 averaged 100 kg ha−1 higher yield than the lines homozygous for the PI 471938 allele. At two independent RF-YLD QTLs on Chr 13 and Chr 17, the lines homozygous for the PI 471938 alleles were 74 to 101 kg ha−1 higher in yield than the lines homozygous for the Hutcheson alleles. Three of the five significant SSR markers associated with RF-YLD were located in a genomic region known for canopy-wilting QTLs, in which the favorable alleles were inherited from PI 471938. The identification of yield-QTLs under the respective rain-fed and irrigated environments provides knowledge regarding differential responses of yield under different irrigation conditions, which will be helpful in developing high-yielding soybean cultivars.
Susceptibility of Commensal E. coli Isolated from Conventional, Antibiotic-Free, and Organic Meat Chickens on Farms and at Slaughter toward Antimicrobials with Public Health Relevance
Authors: Musa Laura , Proietti Patrizia Casagrande , Marenzoni Maria Luisa , Stefanetti Valentina , Kika Tana Shtylla , Blasi Francesca , Magistrali Chiara Francesca , Toppi Valeria , Ranucci David , Branciari Raffaella , Franciosini Maria Pia
Published: November 2021

The spread of resistant bacteria from livestock to the food industry promoted an increase of alternative poultry production systems, such as organic and antibiotic-free ones, based on the lack of antimicrobial use, except in cases in which welfare is compromised. We aimed to investigate the antibiotic susceptibility of commensal Escherichia coli isolated from organic, antibiotic-free, and conventional broiler farms and slaughterhouses toward several antimicrobials critically important for human health. To assess antimicrobial susceptibility, all E. coli isolates and extended spectrum beta-lactamase (ESBL) E. coli were analysed by the microdilution method. The prevalence of tigecycline, azithromycin and gentamicin E. coli-resistant strains was highest in organic samplings. Conversely, the lowest prevalence of resistant E. coli strains was observed for cefotaxime, ceftazidime and ciprofloxacin in organic systems, representing a significant protective factor compared to conventional systems. All E. coli strains were colistin-susceptible. Contamination of the external environment by drug-resistant bacteria could play a role in the presence of resistant strains detected in organic systems. Of interest is the highest prevalence of cephalosporin resistance of E. coli in conventional samplings, since they are not permitted in poultry. Our results suggest that monitoring of antibiotic resistance of the production chain may be helpful to detect “risks” inherent to different rearing systems.
Genomic Features of Salmonella enterica Subspecies houtenae Serotype 45:g,z51:- Isolated from Multiple Abdominal Abscesses of an African Fat-Tailed Gecko, United States, 2020
Authors: Hyeon Ji-Yeon , Helal Zeinab H. , Polkowski Robert , Vyhnal Kristin , Mishra Neha , Kim Junwon , Risatti Guillermo R. , Lee Dong-Hun
Published: November 2021

Salmonella enterica subsp. houtenae (S. houtenae) is a common subspecies in reptiles and has been implicated as a source of serious and life-threatening diseases in humans. Although occurrence and significance of S. houtenae infections have been extensively studied, the genetic features of S. houtenae have remained unknown due to a lack of available high-quality genome sequences. We obtained the complete genome sequence of S. houtenae 45:g,z51:- strain 20-369 isolated from multiple abdominal abscesses of an African fat-tailed gecko (Hemitheconyx caudicinctus) using Nanopore and Illumina sequencing technologies and generated the 4.65Mbp complete genome sequence of the S. houtenae str. 20-369. We annotated and analyzed the genome sequence with the aim to gain a deeper understanding of the genome characteristics associated with its pathogenicity. Overall, this study found several interesting genomic features such as pseudogene formation, virulence gene profile, and novel genomic islands. This study provides basis for an understanding possible genetic mechanism underlying pathogenicity of S. houtenae 45:g,z51:- as well as a high-quality genome reference for future comparison studies.
Pet Owners and Antibiotics: Knowledge, Opinions, Expectations, and Communication Preferences
Authors: Scarborough Ri , Hardefeldt Laura , Browning Glenn , Bailey Kirsten
Published: November 2021

Despite the important role of antimicrobial use in companion animals in the global challenge presented by antimicrobial resistance (AMR), very few studies have quantified pet owner factors that can contribute to suboptimal veterinary antimicrobial use. We conducted an online survey of pet owners, asking about their experiences with veterinarians, their opinions on antibiotic use and knowledge of antibiotics, and their communication preferences regarding judicious prescribing. Just over half (54%) of the 558 pet owners had received antibiotics for their pet at their last non-routine veterinary consultation and most owners were happy (83%) with the antibiotic prescribing decision of their veterinarian. A quarter (25%) indicated that they had been surprised, disappointed or frustrated when a veterinarian had not given their pet antibiotics; 15% had explicitly requested them. Owners placed a higher priority on their pet receiving the most effective treatment than on treatment being cheap or convenient. Most respondents recognized the limitations of antibiotic therapy and the risks associated with antibiotic use, but 50% believed the risks were confined to the treated animal; only a minority was aware of inter-species transfer of bacteria. Pet owners indicated that they would find judicious prescribing messages focused on the direct risks of antibiotics to their pet more compelling than those about public health. Our findings suggest that veterinary communications about responsible antibiotic use should focus on pet owners’ priorities and address or bypass their gaps in understanding regarding antibiotic resistance.
Molecular Characteristics and Antimicrobial Resistance of Salmonella enterica Serovar Schwarzengrund from Chicken Meat in Japan
Authors: Matsui Kaoru , Nakazawa Chisato , Thiri Maung Maung Khin Shwe , Iwabuchi Eriko , Asai Tetsuo , Ishihara Kanako
Published: November 2021

Our previous study revealed that Salmonella enterica serovar Schwarzengrund-contaminated areas of broiler chickens have expanded from West Japan to East Japan. The present study investigated the antimicrobial resistance and molecular characteristics of 124 S. Schwarzengrund isolates obtained from chicken meat produced in East and West Japan from 2008 to 2019. Comparing the isolates obtained in 2008 and 2015–2019, an increase in the proportion of those resistant to kanamycin [51.4–89.7% (p < 0.001)] was observed. In contrast, the proportion of isolates resistant to both streptomycin and tetracycline and those that harbored a 1.0-kb class 1 integron, aadA1, and tetA, significantly decreased from 100% in 2008 to 47.1% in 2015–2019 (p < 0.001). A 1.0-kb class 1 integron containing aadA1, harbored by 78 isolates, was different from that reported in globally distributed S. Schwarzengrund strains (1.9 kb, containing the dfrA12-aadA2 gene cassette). Twenty-five isolates from different product districts and years of isolation were typed as sequence type (ST) 241 with multilocus sequence typing. Our results suggest that S. Schwarzengrund, which contaminates chicken meat in Japan, shares a common ancestor regardless of the product district from 2008 to recent years. Moreover, S. Schwarzengrund ST241 may have spread from western to eastern Japan.
Possible Gender Influence in the Mechanisms Underlying the Oxidative Stress, Inflammatory Response, and the Metabolic Alterations in Patients with Obesity and/or Type 2 Diabetes
Authors: Contreras-Zentella Martha Lucinda , Hernández-Muñoz Rolando
Published: November 2021

The number of patients afflicted by type 2 diabetes and its morbidities has increased alarmingly, becoming the cause of many deaths. Normally, during nutrient intake, insulin secretion is increased and glucagon secretion is repressed, but when plasma glucose concentration increases, a state of prediabetes occurs. High concentration of plasma glucose breaks the redox balance, inducing an oxidative stress that promotes chronic inflammation, insulin resistance, and impaired insulin secretion. In the same context, obesity is one of the most crucial factors inducing insulin resistance, inflammation, and contributing to the onset of type 2 diabetes. Measurements of metabolites like glucose, fructose, amino acids, and lipids exhibit significant predictive associations with type 2 diabetes or a prediabetes state and lead to changes in plasma metabolites that could be selectively affected by gender and age. In terms of gender, women and men have biological dissimilarities that might have an important role for the development, diagnosis, therapy, and prevention of type 2 diabetes, obesity, and relevant hazards in both genders, for type 2 diabetes. Therefore, the present review attempts to analyze the influence of gender on the relationships among inflammatory events, oxidative stress, and metabolic alterations in patients undergoing obesity and/or type 2 diabetes.
Effect of Green Tea Supplementation on Antioxidant Status in Adults: A Systematic Review and Meta-Analysis of Randomized Clinical Trials
Authors: Rasaei Niloufar , Asbaghi Omid , Samadi Mahsa , Setayesh Leila , Bagheri Reza , Gholami Fatemeh , Soveid Neda , Casazza Krista , Wong Alexei , Suzuki Katsuhiko , Mirzaei Khadijeh
Published: November 2021

It is well-established that green tea supplementation has antioxidant properties. However, whether green tea supplementation leads to oxidative stress reduction remains unclear, as clinical investigations on this subject have yielded inconsistent outcomes. Consequently, we aimed to determine the effects of green tea supplementation on oxidative stress in adults. A systematic search of English language publications up to 21 August 2021 was carried out in PubMed, Scopus, Embase, and ISI Web of Science, utilizing pertinent keywords. These searches included randomized controlled trials (RCTs) evaluating the relationship between green tea supplementation, malondialdehyde (MDA), and total antioxidant capacity (TAC) in adults. A random-effects model was used to estimate the weighted mean difference (WMD) and 95% confidence intervals (95% CI). Meta-regression and non-linear dose-response analyses were performed to investigate the association between the dosage of green tea (mg/day) and the duration of the intervention (weeks) with pooled effect size. Sixteen RCTs with seventeen arms including 760 participants met the inclusion criteria. Our results indicated that green tea supplementation had significant effects on TAC (weighted mean difference [WMD]: 0.20 mmol/L; 95% CI: 0.09, 0.30, p < 0.001) and significant heterogeneity between studies (I2 = 98.6%, p < 0.001), which was largely related to gender and body mass index (BMI). Subgroup analysis in TAC identified a significant relationship except with low dose supplementation and obese individuals. No relationship between MDA and green tea supplementation was observed in any subgroups; however, meta-regression analysis revealed a linear inverse association between the dosage and significant change in MDA (r = −2117.18, p = 0.017). Our outcomes suggest that green tea supplementation improves TAC and affects MDA based on the dose of the intervention in adults. Future RCTs with longer durations are needed to expand our findings.
Sulfur Administration in Fe–S Cluster Homeostasis
Authors: Rydz Leszek , Wróbel Maria , Jurkowska Halina
Published: November 2021

Mitochondria are the key organelles of Fe–S cluster synthesis. They contain the enzyme cysteine desulfurase, a scaffold protein, iron and electron donors, and specific chaperons all required for the formation of Fe–S clusters. The newly formed cluster can be utilized by mitochondrial Fe–S protein synthesis or undergo further transformation. Mitochondrial Fe–S cluster biogenesis components are required in the cytosolic iron–sulfur cluster assembly machinery for cytosolic and nuclear cluster supplies. Clusters that are the key components of Fe–S proteins are vulnerable and prone to degradation whenever exposed to oxidative stress. However, once degraded, the Fe–S cluster can be resynthesized or repaired. It has been proposed that sulfurtransferases, rhodanese, and 3-mercaptopyruvate sulfurtransferase, responsible for sulfur transfer from donor to nucleophilic acceptor, are involved in the Fe–S cluster formation, maturation, or reconstitution. In the present paper, we attempt to sum up our knowledge on the involvement of sulfurtransferases not only in sulfur administration but also in the Fe–S cluster formation in mammals and yeasts, and on reconstitution-damaged cluster or restoration of enzyme’s attenuated activity.
Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
Authors: Chen Tao , Luo Siyuan , Wang Xiaoju , Zhou Yiling , Dai Yali , Zhou Lijun , Feng Shiling , Yuan Ming , Ding Chunbang
Published: November 2021

Blumea laciniata is widely used as a folk medicine in Asia, but relevant literature on it is rarely reported. We confirmed that polyphenol extract (containing chlorogenic acid, rutin, and luteolin-4-O-glucoside) from B. laciniata (EBL) showed strong antioxidant ability in vitro. Hence, in this work, we applied Caenorhabditis elegans to further investigate the antioxidant and anti-ageing abilities of EBL in vivo. The results showed that EBL enhanced the survival of C. elegans under thermal stress by 12.62% and sharply reduced the reactive oxygen species level as well as the content of malonaldehyde. Moreover, EBL increased the activities of antioxidant enzymes such as catalase and superoxide dismutase. Additionally, EBL promoted DAF-16, a transcription factor, into the nucleus. Besides, EBL extended the lifespan of C. elegans by 17.39%, showing an anti-ageing effect. Different mutants indicated that the insulin/IGF-1 signaling pathway participated in the antioxidant and anti-ageing effect of EBL on C. elegans.
The Chloride Ion Penetration Mechanism in Basalt Fiber Reinforced Concrete under Compression after Elevated Temperatures
Authors: Lu Limin , Wu Shaohua , Qin Yuwen , Yuan Guanglin , Zhao Qingli , Doh Jeung-Hwan
Published: November 2021

Chloride ion penetration frequently leads to steel corrosion and reduces the durability of reinforced concrete. Although previous studies have investigated the chloride ion permeability of some fiber concrete, the chloride ion permeability of the basalt fiber reinforced concrete (BFRC) has not been widely investigated. Considering that BFRC may be subjected to various exposure environments, this paper focused on exploring the chloride ion permeability of BFRC under the coupling effect of elevated temperatures and compression. Results demonstrated that the chloride ion content in concrete increased linearly with temperature. After exposure to different elevated temperatures, the chloride ion content in BFRC varied greatly with increasing stress. The compressive stress ratio threshold for the chloride ion penetration was measured. A calculation model of BFRC chloride ion diffusion coefficient under the coupling effect of elevated temperatures and mechanical damage (loading test) was proposed.
The Relationship between Dentofacial Vertical Pattern and Bite Force Distribution among Children in Late Mixed Dentition
Authors: AlShammery Deema Ali , AlShuruf Ahmad Mahdi , AlQhtani Nasser , Pani Sharat Chandra
Published: November 2021

Background: Digital bite measurement systems such as the T-Scan III allow for the computerized measurement of occlusal force distribution. This study aimed to establish the relationship between dentofacial vertical pattern and bite force distribution among children in late mixed dentition. Materials and Methods: In total, 86 children (45 male, 41 female) aged between 9 and 11 years with short (n = 28), medium (n = 28), and long (n = 30) facial heights were included in this study. The height, weight, age, and gender were recorded. Occlusal bite force distribution and time of occlusal cycle were recorded using a T-Scan III device (Tekscan Corp. Boston, MA, USA). The bite force distribution was compared among facial types using a One-Way ANOVA and post hoc test, a linear regression model with time of occlusion as dependent variable was developed. Results: No significant differences were observed in occlusion time between genders. Children with long facial height had a significantly lower anterior bite force distribution (p < 0.05) and significantly higher posterior bite force distribution (p < 0.05) than those with average or short facial height. Age, gender, height, and weight had no significant association with time of the occlusal cycle. Conclusion: Children with an increased vertical facial height have a more posterior distribution of force than children with average or short facial heights in the late mixed dentition.