bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study Of English Songs On Teaching Vocabulary At Kindergaten Students Of Lembaga Pendidikan Islam Bustanuddin Galis Pamekasan
Authors: Ratnawati Rinta , Wafi Abdul , Navlia Rusdiana
Published: October 2020

Teaching English to young learners, especially for kindergarten students, is focused on teaching vocabulary because they are still introduced to new language in which it has not ever been learned before. The use of songs in teaching vocabulary for young learners is an effective way to teach English. This study is descriptive qualitative research because (1) It is concerned with context and meaning, (2) the researcher works in natural setting, (3) the researcher is the key instrument in collecting data, (4) the data are presented descriptively, and (5) the data are analyzed inductively. The subjects are kindergarten students and the English teacher. In this study, the instruments used are observation checklist, field note, and interview guide for the four teachers. From the findings, it can be concluded the characteristics of the songs used in teaching vocabulary were the lyrics of the songs were simple and not too long, there was the repetition of the words while singing the songs, the vocabulary used in the lyrics was presented in the meaningful context, the lyrics were based on the theme in the school curriculum, and the rhythm of the songs was fun, The teachers also considered the criteria of the appropriate songs for their students while they were selecting the songs.
Prophetic Parenting dalam Membentuk Akhlak Islami Anak Usia Dini pada Himpunan Wali Santri (HIWASI) RA Al Mansur Bulangan Haji Kecamatan Pegantenan Kabupaten Pamekasan
Authors: Karimah Kiswatul , Ummah MS S. Sumihatul
Published: October 2020

Penelitian ini bertujuan untuk Mengetahui konsep implementasi, serta manfaat dari Prophetic Parenting  dalam Membentuk Akhlak Islami Anak Usia Dini pada Himpunan Wali Santri RA Al Mansur Bulangan Haji Kecamatan Pegantenan Kabupaten Pamekasan.Penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian deskriptif. Data yang diperoleh melelalui wawancara, observasi, dan dokumentasi, informannya yang dianggap menguasai bidang yang diteliti oleh peneliti adalah kepala sekolah, guru, ketua Himpunan wali santri dan wali santri. Sedangkan pengecekan keabsahan data melalui ketekunan, perpanjangan waktu dan trianggulasi.Hasil penelitian menunjukkan bahwa, pertama, konsep Prophetic Parenting  dalam Membentuk Akhlak Islami Anak Usia Dini pada Himpunan Wali Santri RA Al Mansur yaitu dikonsep dengan beberapa materi kegiatan yang salaras dengan bagaimana Rasulullah SAW. mendidik anak yaitu melalui pembiasaan yang baik, penanaman kejujuran, pembiasaan berbahasa Madura halus, menjadi suri tauladan, pemberian perhatian dan kasih sayang, pemberian nasehat, sanjungan dan hukuman, membangun sosial kemasyarakatan anak serta membentuk aktivitas ibadah anak. Kedua,  implementasi Prophetic Parenting  yaitu terdapat pada program Parenting Class, Visiting Home, dan Perayaan Hari Besar Islam. Ketiga manfaat yang dirasakan oleh wali santri dan guru yaitu meningkatkan kesadaran orangtua tentang bagaimana mendidik anak dengan baik dan benar, wali santri bisa lebih memahami bagaimana cara mendidik anak yang mengacu pada bagaimana Rasulullah SAW. mendidik anak, wali santri mendapatkan informasi mengenai program pendidikan anak, silaturahmi, serta bisa mengetahui metode serta bagaimana menerapkan cara Nabi mendidik anak.
Gambaran Self Efficacy Anggota Keluarga Yang Mengalami Stigma Selama Merawat Penderita Gangguan Jiwa Di Kecamatan Buduran, Sidoarjo
Authors: Farina Fara , Yusuf Ah , Wahyuni Sylvia Dwi
Published: October 2020

Pendahuluan: Stigma yang didapatkan penderita gangguan jiwa dari masyarakat juga dirasakan oleh keluarga, mereka akan merasa sedih, malu, takut, dan merasa tidak leluasa melakukan banyak aktivitas diluar. Beban psikologis yang dirasakan oleh keluarga yang merawat dapat mempengaruhi keadekuatan self efficacy dalam menjalani kegiatan sehari-hari. Tujuan dari penelitian ini Menjelaskan gambaran self efficacy keluarga yang mengalami stigma selama merawat penderita gangguan di Kecamatan Buduran, Sidoarjo.Metode: Jenis desain penelitian yang digunakan adalah metode penelitian kualitatif deskriptif fenomenologi dengan partisipan sebanyak 15 orang yang memiliki anggota keluarga dengan gangguan jiwa. Teknik pengambilan data yang digunakan berupa indepth interview. Data dianalisis menggunakan teknik Colaizi. Data didapatkan dengan melakukan wawancara secara mendalam kepada partisipan.Hasil: penelitian ini menghasilkan gambaran self efficacy anggota keluarga yang merawat penderita gangguan jiwa didasarkan pada besaran masalah yang dirasakan berupa stigma masyarakat pada dimensi magnitude, sehingga terbentuk mekanisme koping baik adaptif ataupun maladaptif pada dimensi strength, menghasilkan cakupan perilaku yang cukup terbatas pada dimensi generality, dan harapan para anggota keluarga dalam kesembuhan penderita dan pemaknaan kondisi.Kesimpulan: suatu keluarga yang memiliki sanak-saudara sebagai penderita gangguan jiwa rentan memiliki self efficacy yang tidak adekuat disebabkan adanya perilaku yang tidak wajar dari penderita. Stigma masyarakat yang berkembang turut dirasakan keluarga dan menjadi beban psikologis. Kondisi demikian mampu mempengaruhi gambaran self efficacy anggota keluarga yang merawat penderita gangguan jiwa.
Analisis Hubungan Antara Self Efficacy dan Beban Kerja Akademik dengan Stres Mahasiswa Profesi Ners
Authors: Dini Mitha Permata , Fitryasari Rizki , Panji Asmoro Candra
Published: October 2020

Pendahuluan: Stres pada mahasiswa yang menjalani praktik klinik masih menjadi masalah yang serius karena dapat berdampak terhadap kesehatan dan prestasi akademik. Self efficacy dan beban kerja akademik dapat berpengaruh terhadap stres mahasiswa keperawatan. Penelitian ini bertujuan untuk mengidentifikasi hubungan antara self efficacy dan beban kerja akademik dengan stres mahasiswa profesi ners.Metode: Metode penelitian menggunakan desain literatur review. Pencarian jurnal atau artikel menggunakan database yang terindeks Scopus, ScienceDirect, Google Scholar. Variabel yang digunakan untuk mencari artikel adalah self efficacy, beban kerja akademik, dan stres. Framework yang digunakan untuk mereview adalah PICOS dan kriteria inklusi yang digunakan yakni jurnal berbahasa Inggris dan Indonesia dengan terbitan tahun 2015 sampai 2020. Hasil dari pencarian artikel didapatkan 10 studi yang sesuai dengan kriteria inklusi yang akan di review. Dilakukan analisis narrative dan tabulasi data pada artikel atau jurnal. Judul, abstrak, teks lengkap dan metodologi dinilai untuk menentukan kelayakan artikel atau jurnal.Hasil: Hasil penelitian ini menunjukkan bahwa self efficacy membantu mahasiswa meningkatkan kompetensi sehingga mahasiswa bisa menyelesaikan tugas dan mengambil langkah-langkah praktis dalam situasi yang menantang sehingga hal ini dapat mengurangi stressor dan menurunkan stres. Selain itu, stres dipengaruhi oleh beban kerja akademik, beban kerja yang berlebihan dapat memicu stres. Stres akademik yang terjadi berdampak negatif pada kompetensi dan kinerja akademik mahasiswa.Kesimpulan: Self efficacy membantu mahasiswa dalam mengurangi stres yang dialami selama menjalani praktik klinik. Beban kerja akademik menjadi stressor terbesar bagi mahasiswa keperawatan yang menjalani praktik klinik.
Analysis of Organizational Readiness towards Library 4.0: A Case Study at X Library
Authors: Qomariyah Astutik Nur , Mursidah Eva , Gonti Yeni Anita , Wahyuni Davi
Published: October 2020

Background of the study: The reality of the inevitability of the Industrial Revolution 4.0 era that must be faced by X University and will have an impact on the X Library towards Library 4.0 has become a serious concern at X University. Thus, it is important to conduct a research study on the readiness of the X Library in implementing Library 4.0.Purpose: The purpose of this study is to identify and analyze the readiness of the X library to implement Library 4.0 in the face of the Industrial Revolution 4.0 by referring to the TORC (Theory of Organizational Readiness for Change), in terms of five contextual factors- the policies and procedures, past experience, organizational resources (human resources and technological resources), organizational structure, and organizational culture.Method: The research method used this study is mixed methods, which combine quantitative and qualitative approaches.Findings: The results showed that the level of organizational readiness in implementing Library 4.0 is the mean value of 2,60. This showed that the X Library is not ready, and needs some of work to implement Library 4.0Conclusion: Based on the five contextual factors to measure organizational readiness toward Library 4.0, it showed that almost all of them are the level of not ready, but there is one factor that shows it is quite ready- the policies and procedures. The lack of organizational readiness because leadership is an important key related to contextual factors that affect the readiness of Library X towards Library 4.0.
Management of Accredited Scientific Education Journals in State Islamic Universities
Authors: Albantani Azkia Muharom , Arifin Fatkhul
Published: October 2020

Background of the study: The paradigm shift in the management of scientific journals from print to electronic also has an impact on accreditation policies that were initially oriented towards print scientific periodicals to the accreditation of electronic-based scientific journals.Purpose: This study aims to identify and explain the traditions and innovations in the management of accredited scientific education journals in PTKIN.Method: This research is a qualitative research with the type of field research with the orientation of empirical data collection in the field. The researcher directly reviews the documents related to management, and observes the physical / non-physical facilities used in the management of scientific journals.Findings: The results showed that of the 9 (Nine) accredited education journals of PTKIN before 2018, the most widely cited is Dinamika Ilmu Journal, amounting to 251 (two hundred fifty one) times. This has happened because Dinamika Ilmu Journal has entered the age of 18 (eighteen) years. While the Edukasia Journal is the least quoted, which is 5 (five) times. Even though the age of Edukasia Journal enters the 13th (thirteenth) year.Conclusion: From the 9 (nine) journals, only the Edukasia Journal still maintains to use the Indonesian full text even though it has been nationally accredited. While Edusains Journal, JPI Journal, Tarbiya Journal, and the Dinamika Ilmu Journal use English full text. This was done in order to support the vision of going international for his journals.
The Multifaceted Roles of Copper in Cancer: a Trace Metal Element with Dysregulated Metabolism, but also a Target or a Bullet for Therapy
Authors: Lelièvre Pierre , Sancey Lucie , Coll Jean-Luc , Deniaud Aurélien , Busser Benoît
Published: October 2020

In the human body, Copper (Cu) is a major and essential player in a large number of cellular mechanisms and signaling pathways. The involvement of Cu in oxidation-reduction reactions requires close regulation of copper metabolism in order to avoid toxic effects. In many types of cancer, variations in copper protein levels have been demonstrated. These variations result in increased concentrations of intra-tumoral Cu and alterations in the systemic distribution of copper. Such alterations in Cu homeostasis may promote tumor growth or invasiveness, or even confer resistance to treatments. Once characterized, the dysregulated Cu metabolism is pinpointing several promising biomarkers for clinical use, with prognostic or predictive capabilities. The altered Cu metabolism in cancer cells and the different responses of tumor cells to Cu are strongly supporting the development of treatments to disrupt, deplete or increase Cu levels in tumors. The metallic nature of Cu, as a chemical element, is key for the development of anticancer agents via the synthesis of nanoparticles or copper-based complexes with antineoplastic properties for therapy. Finally, some of these new therapeutic strategies such as chelators or ionophores have shown promising results in a preclinical setting, while others are already in the clinic.
Influence of Unpadded Transplanting on Rice (Oryza sativa L.) Retained Reside of Previous Mustard
Authors: Hossain Mohammad Mobarak , Begum Mahfuza , Rahman Md. Moshiur , Hashem Abul , Bell Richard W.
Published: October 2020

On-farm research was conducted at Gouripur sub-district under Mymensingh district of Bangladesh during boro (mid November-June) season in 2013-14 and 2014-15 to evaluate the performance of unpadded rice cultivation with crop residue retention. The rice var. BRRI dhan28 was transplanted by two tillage practices viz., puddled conventional tillage (CT) and non-puddled strip tillage (ST) and two levels of mustard residues, i.e., no residue (R0) and 50% residue (R50). The experiment had designed in a randomized complete block design with four replications. There were no significant yield differences between tillage practices and residue levels in 2013-14. But in the following year, ST yielded higher grains (5.72 t ha-1), which was about 9.36 % higher compared to CT. The higher grain yield in ST, leading to 22.23% higher BCR compared to CT. Retention of 50% residue increased by 3.15 % yield compared to no-residue, which contributed to 10.58 % higher benefit-cost ratio (BCR). The ST combine with 50 % residue retention yielded the highest grain yield (5.81 t ha-1) which credited to obtain the highest BCR (1.06).
Modeling Combination of Question Order Effect, Response Replicability Effect, and QQ-Equality with Quantum Instruments
Authors: Ozawa Masanao , Khrennikov Andrei
Published: October 2020

We continue to analyze basic constraints on human's decision making from the viewpoint of quantum measurement theory (QMT). As has been found, the conventional QMT based on the projection postulate cannot account for combination of the question order effect (QOE) and the response replicability effect (RRE). This was an alarm signal for quantum-like modeling of decision making. Recently, it was shown that this objection to quantum-like modeling can be removed on the basis of the general QMT based on quantum instruments. In the present paper we analyse the problem of combination of QOE, RRE, and the famous QQ-equality (QQE). This equality was derived by Busemeyer and Wang and it was shown (in the joint paper with Solloway and Shiffrin) that statistical data from many social opinion polls satisfies it. Now, we construct quantum instruments satisfying QOE, RRE, and QQE. The general features of our approach are formalized with postulates which generalize {\it Wang-Busemeyer} postulates for quantum-like modeling of decision making. Moreover, we show that our model closely reproduces the statistics of the famous Clinton-Gore Poll data with a prior belief state independent of the question order. This model successfully removes the order effect from the data to determine the genuine distribution of the opinions in the Poll. The paper also provides a psychologist-friendly introduction to the theory of quantum instruments - the most general mathematical framework for quantum measurements. We hope that this theory will attract attention of psychologists and will stimulate further applications.
Automated Human Activity Recognition by Colliding Bodies Optimization-based Optimal Feature Selection with Recurrent Neural Network (RNN)
Authors: Khatiwada Pankaj , Subedi Matrika , Chatterjee Ayan , Wulf Gerdes Martin
Published: October 2020

— In a smart healthcare system," Human Activity Recognition (HAR)" is considered as an efficient approach in pervasive computing from activity sensor readings. The "Ambient Assisted Living (AAL)" in the home or community helps the people to provide independent care and enhanced living quality. However, many AAL models are restricted to multiple factors that include both the computational cost and system complexity. Moreover, the HAR concept has more relevance because of its applications, such as content-based video search, sports play analysis, crowd behavior prediction systems, patient monitoring systems, and surveillance systems. This paper attempts to implement the HAR system using a popular deep learning algorithm, namely "Recurrent Neural Network (RNN)" with the activity data collected from smart activity sensors over time, and it is publicly available in the "UC Irvine Machine Learning Repository (UCI)". The proposed model involves three processes: (1) data collection, (b) optimal feature learning, and (c) activity recognition. The data gathered from the benchmark repository was initially subjected to optimal feature selection that helped to select the most significant features. The proposed optimal feature selection method is based on a new meta-heuristic algorithm called "Colliding Bodies Optimization (CBO)". An objective function derived from the recognition accuracy has been used for accomplishing the optimal feature selection. The proposed model on the concerned benchmark dataset outperformed the conventional models with enhanced performance.
On Soft Lebesgue Measure
Authors: Goldar Sujay , Ray Subhasis
Published: October 2020

In this article, we introduce the concept of soft intervals, soft ordering and sequences of soft real numbers and study their properties and some interesting results. Also the notion of soft Lebesgue measure on the soft real numbers has been introduced. A correspondence relationship between the soft Lebesgue measure and the Lebesgue measure has been established.
МУЛЬТИСИСТЕМНЫЙ ВОСПАЛИТЕЛЬНЫЙ СИНДРОМ, АССОЦИИРОВАННЫЙ С COVID-19 У ДЕТЕЙ, КАК ИСХОД ЛЕГКИХ ФОРМ ЗАБОЛЕВАНИЯ
Authors: Мелехина Елена , Музыка Анна , Николаева Светлана , Усенко Денис , Понежева Жанна , Мирзонов Владислав , Барыкин Вадим , Новиков Дмитрий , Горелов Александр
Published: October 2020

Согласно данным литературы, новая коронавирусная инфекция COVID-19 у детей развивается реже (до 10% в структуре инфицированных вирусом SARS-CoV-2) и протекает легче, чем у взрослых, а летальные исходы – единичны. Однако у детей, перенесших легкие формы COVID-19, может развиваться мультисистемный воспалительный синдром (МВС). Для МВС характерно поражение респираторного и желудочно-кишечного тракта (боль в животе, рвота, диарея), менингеальные признаки и признаки поражения сердечно-сосудистой системы (неспецифические изменения на ЭКГ по типу миокардита, систолическая дисфункция в сочетании с низким систолическим артериальным давлением, но без развития тромбоза коронарных артерий), а также лейкопения с выраженной лимфопенией, тромбоцитопенией, повышением уровня ферритина и маркеров миокардита. Возможно появление кожной сыпи, хейлита, цервикальной аденопатии и менингизма. Развитие тяжелого МВС у детей может происходить через 2-6 недель после перенесенных легких форм COVID-19. Это диктует необходимость раннего назначения противовирусных средств для экстренной профилактики в очаге инфекции и лечения легких форм болезни у детей согласно действующим клиническим рекомендациям.
Optimization of Slotted Liner in Rubiales Field: Trade-Off between Sand Control, Flow Performance, and Plugging Tendency
Authors: Roostaei Morteza , Cespedes Edgar Alberto Mayorga , Uzcátegui Alberto A. , Soroush Mohammad , Hosseini Seyed Abolhassan , Izadi Hossein , Schroeder Brad , Mahmoudi Mahdi , Gomez Dionis M. , Mora Edgar , Alpire Javier , Torres Joselvis , Fattahpour Vahidoddin
Published: October 2020

Designing and selecting the proper sand control mechanism for horizontal wells in unconsolidated heavy-oil reservoirs tend to be underlooked in some cases. Standalone completions pose some sand control challenges, which could jeopardize the oil production or even lead to critical problems. Massive sand production, screen/formation plugging, hot spots, and mechanical integrity failures are some of the well-known issues. This study attempts to optimize the slotted liner design for horizontal wells in a heavy-oil field in Colombia.

A careful selection of representative core data was made to study the variation of sand particle-size distribution (PSD) within the development area. Reservoir fluid properties were analyzed. Based on PSD variation and current design criteria in the industry, several seamed slotted-liner configurations were proposed as an alternative completion for testing. Later, a series of large-scale sand retention tests (SRTs) were performed to assess the selected alternatives under typical field production conditions. The effects of aperture size and open-to-flow area were investigated to evaluate flow and sand control performance.

This investigation started with a detailed study of the PSD, particle shape variation, and composition of fines in the development area. The PSD then classified into four distinct minor and major sand facies, ranging from medium to very coarse sand with different fines content. Further investigations have shown that current design is only suitable for a limited number of the PSDs, while the overall PSD classes indicate the requirement for wider slot aperture sizes. The results of the SRTs indicated that the flow performance of the screen is mainly controlled by the slot aperture. Choosing the optimized aperture size avoids unacceptable sanding even for the multiphase flow scenarios with gas. Results also indicated that by increasing the aperture size and application of the seamed slots for the studied formation, plugging could be mitigated.

A comprehensive sand control design workflow for cold primary heavy-oil production in horizontal wells is presented in this work. The current study is one of the first that investigates and compares conventional straight slotted liners with seamed slotted liners at a larger scale for this field. Moreover, this study helps to better understand the effect of design parameters of seamed slotted liners on sand control, flow performance, and plugging tendency.
Socio-demographic and clinical characteristics of diabetes mellitus in rural Rwanda: Time to contextualize the interventions? A cross-sectional study
Authors: Munganyinka Charlotte Bavuma , Musafiri Sanctus , Rutayisire Pierre-Claver , Ng’ang’a Loise M , McQuillan Ruth , Wild Sarah H.
Published: October 2020

Background: Existing prevention and treatment strategies target the classic types of diabetes yet this approach might not always be appropriate in some settings where atypical phenotypes exist. This study aims to assess the socio-demographic and clinical characteristics of people with diabetes in rural Rwanda compared to those of urban dwellers.Methods: A cross-sectional, clinic-based study was conducted in which individuals with diabetes mellitus were consecutively recruited from April 2015 to April 2016. Demographic and clinical data were collected from patient interviews, medical files and physical examinations. Chi-square tests and T-tests were used to compare proportions and means between rural and urban residents.Results: A total of 472 participants were recruited (mean age 40.2±19.1years), including 295 women and 315 rural residents. Compared to urban residents, rural residents had lower levels of education, were more likely to be employed in low-income work and to have limited access to running water and electricity. Diabetes was diagnosed at a younger age in rural residents (mean ± SD 32±18 vs 41±17 years; p < 0.001). Physical inactivity, family history of diabetes and obesity were significantly less prevalent in rural than in urban individuals (44% vs 66%, 14.9% vs 28.7% and 27.6% vs 54.1%, respectively; p < 0.001). The frequency of fruit and vegetable consumption was lower in rural than in urban participants. High waist circumference was more prevalent in urban than in rural women and men (75.3% vs 45.5% and 30% vs 6%, respectively; p< 0.001). History of childhood under-nutrition was more frequent in rural than in urban individuals (22.5% vs 6.4%; p< 0.001).Conclusions: Characteristics of people with diabetes in rural Rwanda appear to differ from those of individuals with diabetes in urban settings, suggesting that sub-types of diabetes exist in Rwanda. Generic guidelines for diabetes prevention and management may not be appropriate in different populations.
53 Years Old is A Reasonable Cut-Off Value To Define Young And Old Patients In Clear Cell Renal Cell Carcinoma：A Study Based On TCGA And SEER Database
Authors: Tang Fucai , Lu Zechao , He Chengwu , Zhang Hanbin , Wu Weijia , He Zhaohui
Published: October 2020

Background: The objectives of this study were to screen out cut-off age value and age-related differentially expressed genes (DEGs) in clear cell renal cell carcinoma (CCRCC) from Surveillance Epidemiology and End Results (SEER) database and The Cancer Genome Atlas (TCGA) database. Methods: We selected 45,974 CCRCC patients from SEER and 530 RNA-seq data from TCGA database. The age cut-off value was defined using the X-tile program. Propensity score matching (PSM) was used to balance the differences between young and old groups. Hazard ratio (HR) was applied to evaluate prognostic risk of age in different subgroups. Age-related DEGs were identified via RNA-seq data. Survival analysis was used to assess the relationship between DEGs and prognosis.Results: In this study, we divided the patients into young (n=14276) and old (n=31698) subgroups according to cut-off value (age=53). Age >53 years was indicated as independent risk factor for overall survival (OS) and cancer specific survival (CSS) of CCRCC before and after PSM. The prognosis of old group was worse than that in young group. Eleven gene were differential expression between the younger and older groups in CCRCC. The expression levels of PLA2G2A and SIX2 were related to prognosis of the elderly. Conclusion: 53 years old was cut-off value in CCRCC. The prognosis of the elderly was worse than young people. It remind clinicians that more attention and better treatment should be given to CCRCC patients who are over 53 years old. PLA2G2A and SIX2 were age-related differential genes which might play an important role in the poor prognosis of elderly CCRCC patients.
Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production
Authors: Martani Francesca , Maestroni Letizia , Torchio Mattia , Ami Diletta , Natalello Antonino , Lotti Marina , Porro Danilo , Branduardi Paola
Published: October 2020

BackgroundLipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To develop economically viable microbial processes, the use of residual feedstocks as growth and production substrates is required.ResultsIn this work we investigated sugar beet pulp (SBP) and molasses, the main residues of sugar beet processing, as sustainable substrates for the growth and lipid accumulation by the oleaginous yeast Lipomyces starkeyi. We observed that in hydrolysed SBP the yeast cultures reached a limited biomass, cellular lipid content, lipid production and yield (2.5 g/L, 19.2 %, 0.5 g/L and 0.08 g/g, respectively). To increase the initial sugar availability, cells were grown in SBP blended with molasses. Under batch cultivation, the cellular lipid content was more than doubled (47.2 %) in the presence of 6 % molasses. Under pulsed-feeding cultivation, final biomass, cellular lipid content, lipid production and lipid yield were further improved, reaching respectively 20.5 g/L, 49.2 %, 9.7 g/L and 0.178 g/g. Finally, we observed that SBP can be used instead of ammonium sulphate to fulfil yeasts nitrogen requirement in molasses-based media for microbial oil production. ConclusionsThis study demonstrates for the first time that SBP and molasses can be blended to create a feedstock for the sustainable production of lipids by L. starkeyi. The data obtained pave the way to further improve lipid production by designing a fed-batch process in bioreactor.
SCC: An accurate imputation method for scRNA-seq dropouts based on a mixture model
Authors: zheng yan , Zhong Yuanke , Hu Jialu , Shang Xuequn
Published: October 2020

Background: Single-cell RNA sequencing (scRNA-seq) enables the possibility of many in-depth transcriptomic analyses at a single-cell resolution, it’s already widely used for exploring the dynamic development process of life, studying the gene regulation mechanism, and discovering new cell types. However, the low RNA capture rate, which cause highly sparse expression with dropout, makes it difficult to do downstream analyses.Method: Most current methods use bimodal model to fit the gene expression with overwhelming zero. In this paper, we proposed scRNA-seq complementation (SCC) to solve the dropout problem in scRNA-seq data. Firstly, we find the nearest neighbor cells of every cell. Then we use a mixture model to impute the dropouts of scRNA-seq data. The model can identify the possibility of dropouts and estimates the reasonable gene expression value.Results: Experiment results show that SCC gives competitive results compared to two existing methods while showing superiority in reducing the intra-class distance of cells and improving the clustering accuracy in both simulation and real data.Conclusions: SCC is an effective tool to resolve the dropout noise in scRNA-seq data. The code is freely accessible at https://github.com/nwpuzhengyan/SCC.
Use of T-SPOT.TB for the diagnosis of unconventional pleural tuberculosis is superior to ADA in high prevalence areas: A prospective analysis of 601 cases
Authors: Yang Xinting , zhang jing , Liang Qingtao , Pan Liping , Duan Hongfei , Yang Yang , Li Hua , Guo Chao , Sun Qi , Jia Hongyan , Du Boping , Wei Rongrong , Xing Aiying , Zhang Zongde , Chen Xiaoyou
Published: October 2020

BACKGROUND: Tuberculous pleural effusion (TPE) is the most common extrapulmonary manifestation which still has lasting effect on lung function, however conventional diagnostic tests for TPE register multiple limitations. OBJECTIVES: This study aims to investigate diagnostic efficacy of the interferon gamma release assay (IGRA: T-SPOT.TB) in TPE patients of different characteristics comparing to adenosine deaminase (ADA), in order to clarify its appropriate setting in clinical diagnosis.METHODS: We performed a prospective, single-centre study including all suspected pleural effusion patients consecutively from June 2015 to October 2018. By using receiver operating characteristic (ROC) curves, technical cut-offs for all enrolled participants were determined, and the utility of IGRA for pleural fluid (PF) was analysed. We obtained the independent risk factors using logistic regression analysis for TPE and evaluated the performance of the T-SPOT assay stratified by risk factors in comparison to ADA.RESULTS: A total of 601 individuals was consecutively recruited. The maximum of early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) in the PF T-SPOT assay had the best diagnostic efficiency in our study, with a sensitivity of 83.0% and a specificity of 83.1%. The corresponding cut-off value was 466 SFCs/106 mononuclear cells, which was equal to ADA (0.885 vs 0.887, P=0.957) and superior to PB. Among the TPE patients with low ADA (<40 IU/L), the sensitivity and specificity of PF T-SPOT were still 87.9% and 90.5%, respectively. The utility of ADA was negatively related to increasing age, but the PF T-SPOT test had a steady performance at all ages. Age (<45 yrs; odds ratio (OR) = 5.61, 95% confidence interval (CI) 3.59-8.78; P<0.001), gender (male; OR = 2.68, 95% CI 1.75-2.88; P<0.001) and body mass index (BMI) (<22; OR = 1.93, 95% CI 1.30-2.88; P=0.001) were independently associated with the risk of TB by multivariate logistic regression analysis. Notably, when stratified by risk factor, the sensitivity of PF T-SPOT was superior to the sensitivity (76.5% vs. 23.5%, P=0.016) for ADA and had noninferior specificity (84.4% vs. 96.9%, P=0.370).CONCLUSIONS: In conclusion, the overall potency of the PF T-SPOT assay is equal to that of ADA for diagnosing TPE. In addition, the PF T-SPOT assay can effectively discriminate TPE patients whose ADA is lower than 40 IU/L and is extremely superior to ADA in unconventional TPE patients (age≥45 yrs, female or BMI≥22). The PF T-SPOT assay is an excellent choice to supplement ADA to diagnose TPE.
A novel research method for workshops and co-production of interventions: using a secret Facebook group
Authors: Buelo Audrey , Kirk Alison , Jepson Ruth
Published: October 2020

Background: Co-production of interventions is reliant on good communication and consensus between participants and researchers but attending in-person meetings and workshops is hard for time-constrained groups such as new mums, who may be geographically dispersed without reliable transport. Discussions with a lay advisory group resulted in the decision to hold a workshop over a secret Facebook group. The aim of this study was to test the feasibility a secret Facebook group for co-production activities. In the example presented, the population was women with previous gestational diabetes; the topic was physical inactivity; and the purpose was to develop an acceptable physical activity intervention.Methods: The researchers created a secret Facebook group with content similar to an in-person workshop that sequentially progressed to develop a programme theory for an intervention. The researcher posted 1-2 times per day for 14 days and members of the group were invited to comment and discuss the content. Feasibility and acceptability of the group were analysed using Facebook analytics and a post-workshop survey.Results: Twenty-one participants took part. In total, 521 comments were provided in response to 18 posts of varying types (average = 28.9 comments per post). The total word count of participant comments was 21,142 words. The workshop was viewed positively, with 20 of 21 participants saying they liked the workshop ‘somewhat’ or ‘a great deal’, and felt the group was a safe and open environment to share opinions. When asked if they would take part in something like this again, 15 of 21 said “Yes”. Participants mentioned the format was convenient; it allowed them to reflect on their experiences and they liked helping research progress. Those who say “maybe” said it was difficult finding time and depended on what else was going on.Conclusion: Using a secret Facebook group as a method of co-production or as a workshop in the research process is a feasible and acceptable method. Social media holds significant potential for co-production and involvement in research for populations who are geographically dispersed or time-constrained; with an uncommon condition; or other circumstances where in-person meetings are either not appropriate or not possible.
Knowledge and Attitudes Towards Contraceptives Among Adolescents and Young Adults
Authors: Sharma Aanchal , McCabe Edward , Jani Sona , Gonzalez Anthony , Demissie Seleshi , Lee April
Published: October 2020

Background: Despite endorsements supporting the use of intrauterine devices (IUDs) for adolescents and young adult women (AYA), they have limited knowledge about them Male partners can influence contraceptive decisions, however their perceived knowledge about IUDs is lower than their objective knowledge. We aim to establish current AYA baseline contraceptive knowledge and attitudes so providers can better target their sexual health educational interventions.Methods: Females and males, aged 13 to 23 years old, from our suburban adolescent clinic, completed an anonymous survey that assessed their knowledge and attitudes towards methods of contraception, with an emphasis on the IUD.Results: Completed surveys totaled 130 (99 females/31 males). Demographic results revealed 31.3% Black/African-American, 30.5% Latino/Hispanic, 17.6% White, 3.0% Asian, and 14.5% Other. The majority of participants (80%) were sexually active. The majority (69.5%) stated they/their partner were currently using a contraceptive method; only 2.6% used IUDs. Half of females (56.6%) and 10.1% of males had heard of IUDs. Despite this, male and female participants lacked knowledge regarding specific IUD facts. Of the participants who had used emergency contraception (EC), only 6.4% knew the copper IUD could be used for EC.Conclusion: Contraceptive knowledge deficits, especially regarding the IUD, continue to exist for AYA patients. Many participants stated they required EC despite “satisfaction” with their birth control method(s) and most were unaware that the copper IUD could be used as EC. These discrepancies highlight the importance of comprehensive contraceptive education for AYA patients. Enhanced and consistent contraceptive options counseling can help providers ensure that their AYA patients make well-informed decisions about family planning, thus improving their quality of life.
Association of Adipocytokines with Lipid and Glycemic Profiles in Women with Normal Weight Obesity
Authors: Taheri Ehsaneh , Hosseini Saeed , Qorbani Mostafa , Mirmiran Parvin
Published: October 2020

Background: Individuals with normal weight obesity (NWO) are prone to cardiometabolic disorders. This study aims to investigate the circulating levels of vaspin, leptin and their association with glycemic and lipid profiles in women with NWO.Methods: Forty women with body mass index (BMI) = 18.5-24.9 kg/m2 and fat mass (FM) ≥ 30% were assigned in the NWO group. Thirty age-matched women with identical BMI range and FM < 30% (normal weight non-obese; NWNO) were considered as a control group. In addition to anthropometric measurements, glycemic and lipid profiles and also, circulating levels of leptin and vaspin were measured.Results: The mean ± standard deviation (SD) age of participants was 28.76 ± 4.76 years in the NWO group and 29.23 ± 4.50 years in the control group. The NWO group had higher mean serum levels of insulin (9.02 ± 4.75 vs. 6.24 ± 2.51, P = 0.009), leptin (17.31 ± 8.10 vs. 9.94 ± 4.30, P < 0.001) and homeostatic model assessment of insulin resistance (HOMA-IR) (33.77 ± 20.71 vs. 23.48 ± 10.03, P = 0.009) compared to the NWNO group. Serum level of vaspin was higher in the NWO group compared to the control group (34.82 pg/ml vs. 27.72 pg/ml, respectively, P = 0.12). In NWO group, serum levels of leptin correlated positively with FBS (r = 0.45, P = 0.02), insulin (r = 0.51, P = 0.008), and HOMA-IR (r = 0.46, P = 0.02) and vaspin concentration was positively associated with insulin (r = 0.36, P = 0.02) and HOMA-IR (r = 0.30, P = 0.06).Conclusion: It is concluded that the concentration of insulin and HOMA-IR index were significantly higher in women with NWO compared to NWNO. Higher levels of leptin and vaspin in the NWO group were associated with glycemic profiles.
Dendritic cells transduced with TIPE-2 recombinant adenovirus induces T cells suppression
Authors: Liu Shudong , wang jie , Li Wenyan , Shi Hui , Zhou Changlong , Tang Ge , Zhang Jiangwei , yang zhao
Published: October 2020

Introduction: TIPE-2 has been identified as a negative regulator of both innate and adaptive immunity and is involved in several inflammatory diseases. However, the role of immune suppression of dendritic cells (DCs) transduced with TIPE-2 has not been well studied. Methods: In this study, DCs were transduced with TIPE-2 recombinant adenovirus, and then were cocultured with allogeneic CD4+ or CD8+T cells. The proliferation, cytokine production and activation marker levels of CD4+ or CD8+T cell were detected. Results: The data demonstrated that T cell proliferation, cytokine production and activation marker levels were attenuated after treated with TIPE-2 transduced DCs. Conclusions: These results suggested that TIPE-2 transduced DCs are capable of inducing allogeneic CD4+ or CD8+T cell immune suppression, which provide a promising way for the therapeutical strategies of transplantation or autoimmune diseases.
The miR-200c/FUT4 axis prevents the proliferation of colon cancer cells by downregulating the Wnt/β-catenin pathway
Authors: Cong Jinchun , Yang Chuanjia , Xia Zhixiu , Gong Jian , Zhang Hong
Published: October 2020

BackgroundMicroRNA(miR)-200c has been widely reported to be involved in colon cancer progress. However, the mechanisms of miR-200c in regulating tumor metastasis and growth remain to be fully elucidated. This study aimed to investigate the mechanism of miR-200c targets fucosyltransferase 4 (FUT4) on the proliferation of colon cancer.MethodsThe miR-200c and FUT4 mRNA levels in LoVo and SW480 cells were measured by real-time quantitative polymerase chain reaction. Further, miR-200c mimic, FUT4 siRNA and FUT4 mimic were transfected into cells, separately. Cell counting kit-8, plate colony formation and transwell assays were used to analyse the cells biological behaviour.. Immunofluorescence was used to analyse the Ki-67 expression Moreover, the Wnt/β-catenin pathway-related proteins were detected by western blots. A double luciferase experiment was performed to confirm the relationship between miR-200c and FUT4. In vivo, tumour growth and Wnt/β-catenin pathway-related proteins were also analysed.ResultsIn vitro, the expression of miR-200c and FUT4 were negatively correlated in LoVo and SW480 cells (correlation coefficients were -0.9046 and -0.9236, respectively). MiR-200c overexpression inhibited the proliferation, migration and invasion of LoVo and SW480 cells by downregulating FUT4. The Ki67-positive cells and Wnt/β-catenin signalling pathway-related proteins were reduced in the miR-200c overexpression and FUT4 silencing groups. A dual luciferase reporting system identified FUT4 as the target of miR-200c. The results in vivo were further confirmed the foundation of cells study.ConclusionsIn summary, miR-200c overexpression inhibits proliferation of colon cancer targeting FUT4 to downregulate the Wnt/β-catenin pathway, which promises molecular targets to inhibit metastasis for colon cancer therapy.
High expression of Fibroblast activation protein (FAP) predicts poor outcome in high-grade serous ovarian cancer
Authors: Li Min , Cheng Xue , Rong Rong , Gao Yan , Tang Xiuwu , Chen Youguo
Published: October 2020

Background: High-grade serous ovarian cancer (HGSOC) is a fatal form of ovarian cancer. Previous studies indicated some potential biomarkers for clinical evaluation of HGSOC prognosis. However, there is a lack of systematic analysis of different expression genes (DEGs) to screen and detect significant biomarkers of HGSOC.Methods: TCGA database was conducted to analyze relevant genes expression in HGSOC. Outcomes of candidate genes expression, including overall survival (OS) and progression-free survival (PFS), were calculated by Cox regression analysis for hazard rates (HR). Histopathological investigation of the identified genes was carried out in 151 Chinese HGSOC patients to validate gene expression in different stages of HGSOC.Results: Of all 57,331 genes that were analyzed, FAP was identified as the only novel gene that significantly contributed to both OS and PFS of HGSOC. In addition, FAP had a consistent expression profile between carcinoma-paracarcinoma and early-advanced stages of HGSOC. Immunological tests in paraffin section also confirmed that up-regulation of FAP was present in advanced stage HGSOC patients. Prediction of FAP network association suggested that FN1 could be a potential downstream gene which further influenced HGSOC survival.Conclusions: High-level expression of FAP was associated with poor prognosis of HGSOC via FN1 pathway.
Perceived facilitators and barriers to chronic disease management in primary care networks: a qualitative study
Authors: Foo Chuan De , Surendran Shilpa , Tam Chen Hee , Ho Elaine Qiao Ying , Matchar David Bruce , Car Josip , Koh Gerald Choon Huat
Published: October 2020

Objective: The increasing chronic disease burden has placed tremendous strain on tertiary healthcare resources in most countries, necessitating a shift in chronic disease management from tertiary to primary care providers. Therefore, the Primary Care Network (PCN) policy was promulgated as a model of care to organise private general practitioners (GPs) into groups to provide GPs with resources to anchor patients with chronic conditions with them in the community. As the PCN is still in its embryonic stages, there is a void in research regarding its ability to empower GPs to manage chronic patients effectively. This qualitative study aims to explore the facilitators and barriers for the management of chronic patients by GPs enrolled in the PCN. Design: We conducted 30 semi-structured interviews with GPs enrolled in a PCN followed by a thematic analysis of audio transcripts until data saturation was achieved. Setting: Singapore Results: Our results suggest that PCNs facilitated GPs to more effectively manage chronic patients through 1) provision of ancillary services such as diabetic foot screening, diabetic retinal photography and nurse counselling to permit a “one-stop-shop”, 2) systematic monitoring of process and clinical outcome indicators through a chronic disease registry (CDR) to promote accountability for patients’ health outcomes and 3) funding streams for PCNs to hire additional manpower to oversee operations and to reimburse GPs for extended consultations. Barriers include high administrative load in maintaining the CDR due to the lack of a smart electronic clinic management system and financial gradient faced by patients seeking services from private GPs which incur higher out-of-pocket expenses than public primary healthcare institutions.Conclusion: PCNs demonstrate great promise in empowering enrolled GPs to manage chronic patients. However, barriers will need to be addressed to ensure the viability of PCNs in managing more chronic patients in the face of an ageing population.
How to improve the transition process to independent living for adolescents with profound intellectual disabilities? Experiences from parents and employees.
Authors: Roos Eirik , Sondenaa Erik
Published: October 2020

BackgroundThe transition process from the family home to independent living for young adults with profound intellectual disability (PID) becomes delayed. Those families face challenges that exceed those of typical families such as higher objective and subjective burden, more frequent psychological distress and lower social support. The aim of this study was to explore the collaboration process between parents and employees and identify factors that improve the transition with less burden.MethodsA descriptive qualitative study was undertaken with 18 persons (9 parents and 9 employees) interviewed individually and in groups. In accordance with the municipality`s guidelines, families with profound intellectual disability (PID) child should apply for housing, when the child turns 16. The purpose is to ensure interdisciplinary collaboration, information flow and coordinated services according to family’s needs. The main question in the interviews was ‘What was your experience with cooperation in the transition process, and what would you do to improve this process?’ The interviews were analysed with a thematic approach using systematic text condensation.ResultsThe parents experienced a lack of general information about the ‘housing waiting list’, level of services, and the plan for time of moving from the family home, and how to choose where and whom to live with. Parents described that they had an unsustainable burden of care during the waiting period, and a family crisis caused the allocation of an apartment in a group house. Employees shared challenges to meet families’ wishes, as there were too few group homes. They experienced good collaboration with families and said they offered respite care, due to reduce parents’ burden of care. Employees experienced that PID children developed skills, mastery and degrees of independence after completing a residency at the Folk High School.ConclusionsTo improve the transition process from family home to independent living for young adults with PID, the informants highlighted some factors to reduce the burden of care to families: 1) Systematic follow-up program for families to observe their needs at an early stage 2) More available group houses 3) Information about the housing-priorities of the services and 4) Educational preparing programs to families.