bluuebunny/crossref_metadata_2025 huggingface dataset:
Rule of Law in Tough Times – A Case Study on the Romanian Sanctioning Policy During the COVID-19 Pandemic
Authors: ȘTEFAN Laura , GRAMA Cezara
Published: November 2020

The COVID-19 pandemic was a genuine stress test for societies around the globe. Societal values were put under public scrutiny, while fear reigned supreme allowing large margins of maneuver for governments in taking restrictive measures promising at least to win some time for health systems to adapt to the new challenges. Along with health systems governments, judicial systems and societies at large had to change the way they function to face the pandemic. In this paper we will present a case study on Romania and the usage of sanctioning mechanisms by the Police and Gendarmerie during the state of emergency, March 16–May 14, 2020. We will explore the challenges regarding the adoption of a sound legal basis for restrictive measures in line with the constitutional provision and the actual implementation of these restrictions with a focus on the performance of two enforcement institutions – the Romanian Police and the Gendarmerie – in this process. In times of crisis, or particularly in times of crisis when the government enjoys even more power than usual, the governmental action must be transparent to build trust and ensure that abuses do not happen. Moreover, the data gathered during the first wave of COVID-19 could help shape public policies for the subsequent waves with the view to improve efficiency while possibly decreasing the restrictions of human rights.
Tension between visibility and invisibility: Science communication in new information environments
Authors: Metag Julia
Published: November 2020

The visibility and invisibility of scientific knowledge, its creation, and of scientists are at the core of science communication research. Thus, prominent paradigms, such as the public understanding of science or public engagement with science and technology, have implications for the visibility of scientific knowledge in the scientific community and among the public. This article posits that visibility in science communication is achieved with the availability of scientific knowledge, the approval of its dissemination, and its accessibility to third parties. The public understanding of science and public engagement with science paradigms emphasize different aspects of visibility with the latter focusing on the visibility of the creation of scientific knowledge more than public understanding of science which focuses on the knowledge itself. The digital information environment has engendered new formats and possibilities for visibility but also new risks, thereby creating tensions in science communication.
Potential of development of economic cooperation between Russia and EAEU and CIS countries
Authors: Migranyan A. A.
Published: November 2020

The article examines the peculiarities of the economic situation and the potential for the development of regional integration of Russia and the CIS and EAEU countries. The complexity of the current economic downturn against the backdrop of the COVID-19 pandemic and the turbulence of world energy markets has a mixed impact not only on the economic downturn in the countries of the region, but also on integration processes, their nature and development prospects. In this context, the study of a set of interrelated processes from the point of view of the formation of economic policies of countries, their foreign economic guidelines and goals is relevant. At the same time, the methodology for integrated assessment of the development potential of regional integration is based on the following assumptions: the need to coordinate and develop economic development strategies and geopolitical strategies; creating the attractiveness of the Russian economy as a core of integration; development of common energy markets as the basic platform for integration in the EAEU.
Human resource training for digital economy: current problems and targets
Authors: Trofimova Irina Nikolaevna
Published: November 2020

This article reviews the problem of training a new generation of specialists in the sphere of information and communication technologies. Relevance of the problem is substantiated by the tasks for overcoming technological gap and human resourcing for the digital transformation of national economy. The theoretical and methodological framework is comprised of the provisions that reveal complex and contradictory nature of the digital transformation process due to being affected by various social, cultural, economic and political factors. The key role is assigned to the educational institutions intended for formation of the new set of knowledge, competences and values. The goal consist in determination of factors that define staff requirements of the information and communication technologies sector (ICT. It is demonstrated that targets in human resource training for the information and communication technologies sector rely on the overall evaluation of economic situation and do not take into account a number of specific factors. It is underlined that the key characteristics of the Russian ICT sector consist in its heterogeneity and dependence on the general trends in the economy and job market, and staff shortage is substantiated by the outcome of previous stage in the development of ICT sector and specificity of the country's economy. In conclusion, the author emphasizes the importance of differentiated approach towards determination of the need and requirements of training of digital personnel in the context of isolated branches, regions and development trends of with regard of information and communication technologies.
Hubungan religiusitas, depresi, dan kecemasan Matematika Mahasiswa
Authors: Purnomo Andreas Wisnu Adi , Loekmono J.T. Lobby
Published: November 2020

Penelitian ini bertujuan untuk mengetahui ada tidaknya hubungan yang signifikan antara variabel religiusitas, depresi dan kecemasan matematika secara bersamaan pada mahasiswa Fakultas Teknik Informatika Universitas Kristen Satya Wacana (FTI UKSW) angkatan 2017 penerima beasiswa dari pemerintah Kabupaten Bengkayang. Penelitian ini berjenis penelitian korelasional dengan jumlah responden sebanyak 34 individu. Teknik sampling yang digunakan adalah Total sampling, yaitu semua populasi dijadikan sampel. Teknik analisis yang digunakan ialah korelasi ganda dengan koefisien signifikansi Sig< 0,05Â  dinyatakan ada hubungan signifikan. Berdasarkan hasil analisis didapati tidak ada hubungan yang signifikan antara variabel religiusitas, depresi dan kecemasan matematika secara bersamaan. Hal ini dilihat dari koefisien signifikansi korelasi berganda senilai 0,601> 0,05.Â Abstract: This study aims to determine whether there is a significant relationship between the variables of religiosity, depression and math anxiety simultaneously in the Satya Wacana Christian University (SWCU FTI) Informatics Engineering students class of 2017 who are scholarship recipients from the Bengkayang Regency government. This research is a correlational research type with the number of respondents as many as 34 individuals. The sampling technique used was total sampling, in which all populations were sampled. The analysis technique used is multiple correlation with a significance coefficient of Sig <0.05, it is stated that there is a significant relationship. Based on the results of the analysis, it was found that there was no significant relationship between the variables of religiosity, depression and math anxiety simultaneously. This can be seen from the multiple correlation significance coefficient of 0.601> 0.05.
Pemberdayaan Remaja Masjid di masa pandemi Covid-19 melalui workshop dan simulasi Konseling Sebaya
Authors: Sari Ratna , Saleh M Nurul Ikhsan , Rahman Diana Nur , Aisah Anita
Published: November 2020

Pandemi Covid-19 membuat ruang gerak semua orang menjadi terbatas, termasuk pada diri remaja. Remaja yang semestinya bebas untuk bersosialisasi dan berinteraksi, kini terbatasi oleh ruang-ruang online. Remaja disibukkan dengan sekolah online yang membuat lebih sibuk di dunia maya daripada di dunia nyata. Hal ini, tidak hanya menimbulkan kebosanan bagi remaja, melainkan juga berefek pada kesehatan mental remaja, sehingga membutuhkan rekan yang dapat membantu permasalahan yang dialami. Penelitian ini bermaksud untuk mengkaji efektifitas metode workshop dan simulasi sebagai wahana pemberdayaan remaja masjid dalam peningkatan skill konselor sebaya di masa pandemi Covid-19. Pendekatan penelitian mengkombinasikan metode kuantitatif dan kualitatif atau mixed method. Metode pengumpulan data berupa observasi serta wawancara terstruktur, pretest dan posttest. Responden dalam penelitian ini adalah remaja masjid yang berjumlah 22 orang. Data kuantitatif dianalisis dengan statistik deskriptif teknik frekuensi. Adapun data kualitatif dianalisis menggunakan teknik Miles dan Huberman, yaitu; reduksi data, verifikasi data dan penyajian data. Hasil dari penelitian ini adalah berupa peningkatan pengetahuan dan kompetensi yang ditunjukkan remaja masjid setelah mengikuti rangkaian workshop konselor sebaya. Responden yang memiliki pemahaman yang baik pada materi posttest sebesar 87% dan dalam simulasi responden mampu melakukan praktik konseling sebaya sesuai panduan.Â Abstract: The Covid 19 pandemic limited everyone's space, including adolescents. Online spaces now limit youths who should be free to socialize and interact. Adolescents are preoccupied with online schools, which make them busier in cyberspace than in the real world. This case causes boredom for adolescents and affects their mental health, thus requiring partners to help their problems. This study intends to examine the effectiveness of workshop and simulation methods to empower mosque youth in increasing peer counselor skills during the Covid-19 pandemic. The research approach combines quantitative and qualitative methods (mixed method). The data collection methods were observation, a structured interview, pretest and posttest. Respondents in this study were 22 mosque youths. The quantitative data were analyzed by using descriptive statistics with frequency technique. The qualitative data were analyzed using the Miles and Huberman technique consisting of data reduction, data verification, and data presentation. This study's result is increased knowledge and competence shown by mosque youth after participating in peer counselor workshops and simulations. Respondents who had a good understanding of the posttest material were 87%, and in the simulation, respondents were able to practice peer counseling according to the guidelines.
TRADISI BERSIH KALI (STUDI KEARIFAN LOKAL DAN POTENSINYA SEBAGAI SUMBER PEMBELAJARAN IPS SD
Authors: Sunyoto S
Published: November 2020

Penelitian ini bertujuan untuk menganalisis dan mendeskripsikan nilai kearifan lokal yang terkandung di dalam tradisi bersih kali di Desa Baosan Kidul Kecamatan Ngrayun Kabupaten Ponorogo dan potensinya sebagai sumber pembelajaran IPS SD. Penelitian ini menggunakan pendekatan kualitatif etnografi dengan memanfaatkan sumber data primer dan sumber data sekunder. Informan ditentukan dengan teknik purposive sampling. Data primer diperoleh dari informan melalui wawancara, observasi dan pencatatan dokumentasi. Sedangkan analisa data analisis data kualitatif menurut Miles dan Huberman yang meliputi reduksi data (data reduction), penyajian data (data display), serta penarikan kesimpulan dan verifikasi (conclusion drawing/verification). Hasil penelitian memberi gambaran bahwa Tradisi Bersih Kali adalah sebuah tradisi turun temurun yang dimiliki oleh masyarakat Desa Baosan Kidul, yang memiliki nilai-nilai kearifan lokal diantaranya: tanggung jawab, rela berkorban, persatuan atau kerukunan, gotong royong, hormat menghormati, religi, spiritual, dan estetika. Oleh karenanya Tradisi Bersih Kali ini berpotensi sebagai salah satu sumber pembelajaran kajian Mata Pelajaran IPS kelas 5 Sekolah Dasar.

 

 
Modeling of dynamic mosaicism on ring chromosomes in human fibroblasts and IPSCS
Authors: Никитина Т.В. , Кашеварова А.А. , Гридина М.М. , Хабарова А.А. , Мензоров А.Г. , Яковлева Ю.С. , Васильев С.А. , Пристяжнюк И.Е. , Минина Ю.М. , Распопова М.А. , Дериглазов Д.А. , Серов О.Л. , Лебедев И.Н.
Published: November 2020

Митотическая нестабильность кольцевых хромосом может приводить к появлению клеточных клонов с различной генетической структурой. В качестве модели нестабильности кольцевых хромосом в митозе мы использовали фибробласты от пациентов с r(8), r(13), r(18) и r(22) и полученные из них индуцированные плюрипотентные стволовые клетки (ИПСК). Линии ИПСК с r(22) имели относительно стабильный кариотип на протяжении десятков (до 60) пассажей и сохраняли неизменную структуру кольцевой хромосомы. Кариотип линий ИПСК с r(8) и r(18) на ранних пассажах стабильный, планируется его изучение на поздних пассажах. Наибольшее разнообразие кариотипа выявлено в линиях ИПСК с r(13), в которых наблюдали различные перестройки и выраженную клеточную гетерогенность. Определение факторов, влияющих на митотическую стабильность кольцевых хромосом, может иметь значение для консультирования пациентов.

Mitotic instability of ring chromosomes can lead to the appearance of cell clones with different genetic structure. IPSCs from fibroblasts of patients with r(8), r(13), r(18), and r(22) were used as a model of ring chromosomes mitotic behavior. Karyotypes of iPSC lines with r(8) and r(18) have so far been evaluated only in the early passages, lines with r(22) have maintained a relatively stable karyotype up to 60 passages. The occurrence of rearrangements and cellular heterogeneity was found characteristic for r(13) iPSCs. The determination of factors affecting the ring chromosomes mitotic stability would be beneficial for the patient’s prognosis.
Clinical and molecular-genetics diagnosis of microdeletion Xp11.4
Authors: Миньженкова М.Е. , Маркова Ж.Г. , Маркова Т.В. , Шилова Н.В.
Published: November 2020

Представлены клинические и молекулярно-генетические результаты обследования девочки с задержкой психомоторного, речевого и физического развития, микроцефалией, гипоплазией моста и мозжечка. Хромосомный микроматричный анализ позволил выявить делецию Хр11.4, затрагивающую ген CASK, который ассоциирован с клиническими проявлениями MICPCH-синдрома (mental retardation and microcephaly with pontine and cerebellar hypoplasia).

Сlinical and molecular-genetic study results of a girl with developemental and psychomotor delay, lack of speech development, microcephaly, and pontocerebellar hypoplasia are presented. Chromosomal microarray analysis revealed a deletion of Xp11.4 affecting the CASK gene that is associated with clinical manifestations of MICPCH-syndrome.
Klinefelter syndrome: low level and tissue mosaicism
Authors: Требка Е.Г.
Published: November 2020

Представлены результаты исследования FISH лимфоцитов крови и буккального эпителия 9 пациентов с синдромом Клайнфельтера и азооспермией.

The results of FISH study of lymphocytes and buccal epithelium of 9 patients with Klinefelter syndrome and azoospermia are presented.
Direct and reverse ontogenetics of chromosomal diseases
Authors: Лебедев И.Н.
Published: November 2020

Представлен анализ современных тенденций исследований на стыке молекулярной цитогенетики, вспомогательных репродуктивных технологий и клеточного репрограммирования, направленных на моделирование патогенеза хромосомных заболеваний в динамике индивидуального развития.

Current trends in molecular cytogenetics, assisted reproductive technologies and cell reprogramming for the revelation of chromosomal diseases pathogenesis are reviewed.
A clinical case of prenatal trisomy 2
Authors: Талантова О.Е. , Серебрякова Е.А. , Малышева О.В. , Тихонов А.В. , Шабанова Е.С. , Ефимова О.А. , Чиряева О.Г. , Прохорова В.С. , Глотов А.С.
Published: November 2020

Представлен редкий случай трисомии 2, подтверждённый пренатально на сроке 18/19 недель беременности методом хромосомного микроматричного анализа (arrayCGH) ворсин плаценты.

A rare case of trisomy 2 is presented. Prenatally at 18/19 weeks of pregnancy, confirmed by the method of chromosome microarray analysis (arrayCGH) of placental villi.
Genetic counselling for patients with fetal sex chromosome aberrations
Authors: Тарасова Ю.А. , Гнетецкая В.А. , Ижевская В.Л. , Мельникова Л.Л.
Published: November 2020

Распространение НИПТ с оценкой риска аберраций половых хромосом привело к увеличению количества пациентов, нуждающихся в генетическом консультировании в отношении этих заболеваний. Анализ факторов, влияющих на репродуктивный выбор семьи, необходим для улучшения качества предоставляемой семье информации.

The spread of NIPTs with risk assessment of sex chromosome aberrations has led to an increase in the number of patients requiring genetic counselling for these pathologies. Analysis of factors influencing family reproductive choices is necessary to improve the quality of information provided to families.
The chromosomal microarray analysis of abortive material in pregnancy loss
Authors: Панченко Е.Г. , Канивец И.В. , Романова И.И. , Киевская Ю.К. , Кудрявцева Е.В. , Пьянков Д.В. , Коростелев С.А.
Published: November 2020

Цель исследования - оценить распространенность и типы хромосомных аномалий (ХА) в абортивном материале за период с 2015 по 2019 гг. Методом хромосомного микроматричного анализа был исследован 2201 образец ДНК, выделенной из абортивного материала при неразвивающейся беременности. ХА были обнаружены в 49,57% случаев, из них анеуплоидии, в том числе нескольких хромосом и мозаичные формы, составляют 79,65%, триплоидия - 10,72%, другие ХА, возможно имеющие клиническое значение, - 8,62%, тетраплоидия - 1,01%. Таким образом, хромосомный микроматричный анализ может быть рекомендован как рутинный метод для поиска несбалансированных ХА в абортивном материале при невынашивании беременности.

Study objective is to assess the prevalence and pattern of chromosomal abnormalities (CAs) in products of conception (POC) for the period from 2015 to 2019. Materials and Methods: 2201 samples of POC were studied by the chromosomal microarray analysis. Study Results: CAs were detected in 49.57% of cases, of which aneuploidy, including several chromosomal and mosaic forms, were detected in 79.65%, triploidy - 10.72%, other CAs with possible clinical significance - 8.62%, tetraploidy - 1.01%. Conclusion: chromosomal microarray analysis can be recommended as a routine method for searching of unbalanced CAs in POC.
Noninvasive prenatal screening for fetal aneuploidy (NIPS): usage for obstetric and gynecological care
Authors: Барков И.Ю. , Шубина Е. , Каретникова Н.А. , Трофимов Д.Ю.
Published: November 2020

Используемый в настоящее время в России пренатальный скрининг хромосомной патологии основан на косвенных маркерах и имеет ограничение по чувствительности и специфичности. Поэтому более перспективным является применение неинвазивного пренатального ДНК-скрининга анеуплоидий (НИПС). Целью данной работы являлась оценка возможности применения полногеномного НИПС при оказании акушерско-гинекологической помощи. Проведена валидация ДНК-скрининга на образцах с известными результатами пренатальной инвазивной диагностики (N=1134). Доказана возможность транспортировки и хранения образцов (N=477). Проведено отсроченное исследование аликвот плазмы через год после первоначального исследования (N=70). Оценены факторы, которые могут влиять на результаты НИПС - доля плодовой ДНК, индекс массы тела (ИМТ), срок беременности, мозаицизм и другие.

Widely performed in Russia prenatally first-trimester screening is based on secondary markers of pathology and has limited sensitivity and specificity. The most promising alternative is the use of noninvasive prenatal screening for fetal aneuploidy (NIPS). This study aims to validate the possibility of NIPS usage for obstetric and gynecological care. DNA screening was validated on samples with known results of invasive prenatal diagnostics (N=1134). It was proven that it is possible to store and transport the samples (N=477). We studied plasma aliquot samples after one year of storage (N=70). Factors that can influence the NIPS results were also evaluated: the proportion of fetal DNA, body mass index (BMI), gestational age, mosaicism, and others.
Application of nipt by ngs sequencing in D.O. Ott research institute for obstetrics, gynaecology and reproductology
Authors: Козюлина П.Ю. , Вашукова Е.С. , Моршнева А.В. , Двойнова Н.М. , Тарасенко О.Е. , Талантова О.Е. , Коротеев А.Л. , Иванов Д.О. , Серебрякова Е.А. , Шабанова Е.С. , Пендина А.А. , Тихонов А.В. , Чиряева О.Г. , Ефимова О.А. , Иващенко Т.Э. , Баранов В.С. , Глотов А.С.
Published: November 2020

Представлены результаты оригинальной технологии использования неинвазивного пренатального скрининга на основе полногеномного секвенирования внеклеточной ДНК (вкДНК) плода в материнской крови. Охарактеризованы основные параметры технологии и предложены новые возможности ее использования.

The results of the original technology of non-invasive prenatal screening based on genome-wide fetal DNA (cfDNA) sequencing of the fetus in maternal blood are presented. The main parameters of the technology are characterized and new possibilities for its use are proposed.
Siamese twin pregnancy in peritoneal dialysis : first case in the world
Authors: El Hamssili D , Bouattar T. , Bayahia R. , Benamar L.
Published: November 2020

Introduction: The probability of conception is low in peritoneal dialysis (PD), and the probability of successful pregnancy is even lower. Nearly 60 years after the first reported case of successful pregnancy in a dialysis patient, many questions about pregnancy during dialysis remain unresolved, namely the required dialysis dose, the follow-up rhythm, the choice of the the most appropriate dialysis technique.

Case report: We report the first case worldwide of twin Siamese pregnancy in a patient on continuous ambulatory peritoneal dialysis (CAPD). The pregnancy was diagnosed at 7 weeks of amenorrhea (WA). The patient was initially under 2 exchanges per day, with 2-liter bags of Dianealof 1.36% glucose. As soon as the pregnancy was discovered, the prescription was modified, putting the patient under 3 exchanges a day with the same concentrates. The infusion volume was maintained at 2 liters up to 18 WA , and then reduced to 1.5 liters due to patient discomfort and abdominal hyperpressure. Daily ultrafiltration ranged from 180 to 800 ml with 1 liter diuresis. The blood pressure was well controlled, with an average of 125/80 mmHg, without need of  antihypertensive drugs. On a biological level, the Kt / V was 2.2, nPCR was 0.8, and the weekly clearance was 80 L / 1.73 m². The average hemoglobin was 11.5 g / dl under erythropoietin 9000 units per week. At 20 WA, obstetrical ultrasound revealed a mono-chorionic, mono-amniotic twin pregnancy. Due to high maternal and fetal risks, closer obstetrical control was indicated. At 24 WA, morphological obstetrical ultrasound coupled with Doppler examination of the uterine and umbilical arteries favored the diagnosis of mono-amniotic mono-chorionic twin pregnancy with strong suspicion of Siamese contiguous to the pelvis, with a cerebral malformation in one of the fetuses. The patient experienced intense contractions and pelvic pain leading to an emergency cesarean section with two surviving Siamese twins, contiguous to the pelvis, weighing 900g, but who died one day after delivery.

Conclusion: Pregnancy during dialysis is at high maternal and fetal risks. It is necessary to prepare and follow this pregnancy to optimize the chances of success. This implies an adaptation of the medical treatment and dialysis prescription, including the volume and modality of exchanges. The same holds true for gynecological and obstetrical follow-up, which must be regular and strict, in order to prevent any maternal-fetal complications.
Why do we have to use new low flux dialysis machine for daily home dialysis
Authors: Fessi Hafedh
Published: November 2020

After a decline at the end of 90’s, Home Hemodialysis recently found a second breath. This renewal doesn’t only concern France, but also the US and Western Europe (GB, Italy and Spain).Several studies confirm that low flux dialysat machines deliver adequate dialysis dose, based on high dialysat saturation and low daily ultrafiltration rate. Frequent sessions avoid long interdialytic interval. They also are easy to use, allow easier session management with more flexibility and mobility.Daily hemodialysis with low dialysat flux responds to new goals: improving quality of life reaching better cardiovascular parameters and reduce recovery time post dialysis.Better patient adhesion to the treatment with low flux dialysat machines allows a decrease in barriers to home memodialysis development.It gives a good opportunity today to improve dialysis quality. The role of patient care partner is determinant
Les pièges des bilans de qualité de dialyse et nutrition en dialyse péritonéale
Authors: Padernoz-Lavallée Marie-Christine , Veniez Ghislaine
Published: November 2020

Le RDPLF met à la disposition de ses membres différents modules de suivi et d’évaluation du traitement par DP. Le « Module nutrition et Dialyse Adéquate » est l’un des plus utilisés. Les résultats de ce module sont criblés, vérifiés, avant d’être enregistrés dans la base de données statistique du Registre. Nous présentons les principaux problèmes rencontrés lors des contrôles de qualité des données.

English abstract

The RDPLF makes available to its members different modules for monitoring and evaluating treatment by PD. The Nutrition and adequacy module is one of the most used. The results of this module are screened and verified before being recorded in the registry database. We present the main problems encountered during the data quality controls.

Cette revue est mise à disposition selon les termes de la Licence Creative Commons Attribution 4.0 International.
Données 2016-2017 incluses dans le registre d'hémodialyse à domicile du RDPLF : comparaison des pratiques en France et Belgique francophone.
Authors: Cougnet Philippe
Published: November 2020

En 2013, le RDPLF a inclus un nouveau module afin de récolter et d’analyser les informations liées à l’hémodialyse à domicile (HDD). On peut estimer que ce registre inclut des données pour environ un tiers des patients traités en HDD en France et la quasi-totalité des patients suivis en Belgique francophone. Nous rapportons ici une comparaison des pratiques concernant l’HDD entre nos deux pays à la lumière des données récoltées dans le module HDD en 2016 et 2017. Le but de cette analyse comparative entre la France et la Belgique est de mettre en évidence les différences nationales qui pourraient exister dans la prise en charge des patients en HDD.

English abstract :

A new module about home hemodialysis (HHD) has been created by the RDPLF in 2013. Though not exhaustive, this module catches up to one third of the French HHD patients and almost all HHD patients from the French-speaking part of Belgium, respectively. We here report on some comparisons between HHD practices within both countries through the 2016 and 2017 annual reports. The aim of this comparative analyis is to highlight some different approaches in both care and management of the dialysis technique itself, mode of vascular access, fistula ponction and dialysis supervision for our HHD patients.

Cette revue est mise à disposition selon les termes de la Licence Creative Commons Attribution 4.0 International.
Comment faire accepter un résumé ou abstract dans un congrès.
Authors: Lanot Antoine , Verger Christian
Published: November 2020

La communication médicale passe par la présentation orale ou écrite de travaux.

Pour assurer un progrès continue de la médecine, Il est capital que les professionnels de santé, quel que soit leur mode d’exercice ou profession, partagent leurs connaissances et expérience. En dehors des réseaux sociaux les principaux supports formels sont la présentation orale lors d’un congrès ou la rédaction d’un article dans une revue. Les deux modes de communication sont soumis à l’accord d’un comité de lecture qui décide de l’opportunité d’accepter le travail. Afin d’aider nos jeunes lecteurs et lectrices, nous rappelons dans ce numéro les règles de soumission d’un résumé pour un congrès. Dans un prochain  numéro, un article sera dédié à la soumission d’un article dans une revue.
What do we learn about the “Anemia Module” of the French language Peritoneal Dialysis ? Interest and Results
Authors: Issad Belkacem , Griuncelli Mireille , Verger Christian , Rostoker Guy
Published: November 2020

Background: Anemia is commonly observed in patients with chronic kidney disease (CKD) as soon as the glomerular filtration rate falls below than 30 ml/min. CKD patients frequently have iron deficiency. The use of both erythropoiesis-stimulating agents (ESA) and iron therapy is the backbone of anemia management in CKD. For this reason, an adequate iron supply is mandatory to achieve the optimal therapeutic benefit of erythropoiesis stimulating agents (ESAs). Many groups agree that anemia in peritoneal dialysis (PD) patients is less severe than in hemodialysis (HD) patients and that there are important differences in treatment practices for anemia between PD and HD patients.

Methods: Analysis of the Anemia module of the French Language Peritoneal Dialysis Registry (RDPLF) register from the database set up in 2005 with a study of the period 2010-2017.

Results: Data from 568 patients who participated in the Anemia module were analysed during the 2010-2017 follow-up period. Their median age were 71 years, 42% were female, median dialysis vintage was 13 months, 40,5% of patients had diabetes mellitus, 74% of patients were treated with ESA, 23% were on oral iron and only 11% have received intravenous iron. In terms of biological assessment, the average hemoglobin level was close to 12 g/dl and median CRP was close to 5 mg/l. For the iron balance, ferritin reached an average level of 270 µg/l in 2013 and stabilized in 2017 at 200 µg/l. The transferrin saturation coefficient always fluctuated between 23 % and 25 % from year 2010 to year 2017.

Conclusion: The results of the Anemia module of RDPLF register appear to be in line with the target values of the ERA-EDTA latest European guideline on anemia (ERBP 2013) and show the low use of intravenous iron in PD (usually as second line therapy).
Exit site infection in peritoneal dialysis : predictive factors for adverse outcome
Authors: Silva Filipa Sofia , Tavares Joana , Correia Sofia O , Freitas Cristina , Santos Olivia , Carvalho Maria João , Malheiro Jorge , Cabrita António , Rodrigues Anabela
Published: November 2020

Infection-related complications in patients on peritoneal dialysis (PD) is a leading complication.

Our aim was to evaluate the type and natural course of ESI events in a cohort of PD treated in last decade of our PD program.

Registry data of ESI events (n=126, in 74 patients) were retrieved. ESI protocols followed standard international guidelines. A systematic quality control is performed.

               The median follow-up was 29.1 (14.0-47.4) months. In this population the adverse outcomes of TI rate and peritonitis rate was 0.12 and 0.13 patient/year, respectively.                Male sex (0.048), older age (0.007) and Staphylococcus aureus (SA) agent (0.006) were predictive of TI while non-optional PD and lower levels of albumin were predictive of peritonitis.                 After grouping the ESI events according to the date of the occurrence of infection (group 1: 2008 to 2012, group 2: 2013 to 2017 and group 3: 2018) a substantial increase of TI in 2018 was evident (P <0.001 when comparing group 3 vs 1 and 0.005 when comparing group 2 and 3).               When ESI occurs simultaneous with TI, the probability of not reaching cure is 65%. Drop-out occurred in 50% of ESI without peritonitis vs 86% with peritonitis (P <0.001). SA is the microorganism most implicated in the failure to heal (P 0.002) and drop-out (P 0.010).

In spite of a number of efforts to reduce ESI, a regular audit still point to the need for protocols review in order to avoid adverse outcomes. Focused training of patients is mandatory but also prophylaxis and antibiotic protocols deserve improvement.
Revue de presse des jeunes néphrologues : 3e trimestre 2019 de la Dialyse à Domicile
Authors: Nodimar Céline , Seret Guillaume , Vrigneaud Laurence , Bataille Stanislas , Carceles Odette , Gosselin Morgane , Lanot Antoine , Lavainne Frederic , Morinière Beaume Julie Morinière
Published: November 2020

Un groupe de néphrologues issus du Club des jeunes Néphrologues sélectionne chaque mois à tour de rôle un article de la littérature internationale en rapport avec la dialyse à domicile, Dialyse Péritonéale ou Hémodialyse à Domicile, et en propose un résumé en français et son analyse. Ces résumés sont librement téléchargeables sur le site du RDPLF à l’adresse : https://www.rdplf.org/biblio.html. Depuis décembre 2018 ceux sélectionnés au cours du trimestre par ces néphrologues sont publiés sous leur nom dans la présente rubrique du Bulletin de la Dialyse à Domicile.
Buttonhole: requirements and experience
Authors: Charytoniuc Krystyna
Published: November 2020

Home hemodialysis requires total autonomy from the patient, who punctures himself.

To this end, the vascular approach must be of good quality and the puncture technic should be easy to handle.

A recognized approach is the "Buttonhole" technique. An easy technique, it encourages patients to continue their dialysis at home.

But the risk of arterio-venous fistula (AVF) infection related to the puncture of the "Buttonhole" is very high. However, this technique has many positive points for the AVF itself, for the patients and for the caring teams.

We looked for ways to improve this technique and eliminate as much as possible the risk of infection and to maintain the functionality of the fistula. We present our experience in this field.
Dialyse péritonéale et pandémie COVID-19
Authors: Verger Christian , Dratwa Max , Chanliau Jacques , Durand Pierre-Yves
Published: November 2020

L’épidémie à Coronavirus COVID-19, qui touche la planète entière, oblige chaque pays et leurs habitants à adapter leurs modes de vie afin, à laLfois, de se préserver et de préserver son entourage. La prise en charge des personnes souffrant d’une maladie chronique associée doit évoluer durant cette période en fonction des pathologies et du lieu de traitement.

Rapidement, en sus des directives ministérielles, les sociétés savantes ont proposé des recommandations qu’il importe à chacun d’adapter en fonction des possibilités et de l’environnement d’exercice. Certaines sont synthétiques et générales, d’autres concernent des groupes plus particuliers d’individus.

Nous citerons ainsi les recommandations (en Anglais) de l’ERA-EDTA :https://www.era-edta.org/en/wp-content/uploads/2020/03/200311_Press-Release_SARS-CoV-2.pdfL’expérience de Wuhan en hémodialyse : https://doi.org/10.1101/2020.02.24.20027201Les recommandations générales de la SFNDT : https://www.sfndt.org/sites/www.sfndt.org/files/medias/documents/Conseils%20SFNDT%20COVID-19%20-%20090320%20vDef.pdf

Peu de recommandations étaient fournies concernant les patients traités par dialyse péritonéale en dehors de celles de l’ISPD récemment publiées en plusieurs langues: https://ispd.org/strategies-covid19/ (traduction française réalisée par le RDPLF)

La France et certains pays francophones se caractérisent aussi par une proportion importante de patients en dialyse péritonéale non autonomes assistés par infirmière. Les responsables du Registre de Dialyse Péritonéale de Langue Française (RDPLF) ont rédigé, en accord avec la Société Francophone de Néphrologie Dialyse et Transplantation (SFNDT) des recommandations, volontairement simples, et spécifiques du mode de prise en charge dans nos régions.

Ces recommandations complètent celles précédemment citées et ne concernent que le suivi du patient lors de son maintien à domicile pour le protéger et protéger son entourage de toute contamination.

Il appartient à chaque professionnel de s’inspirer de ces recommandations et de les adapter en fonction de ses possibilités..
Approche économique d e l’hémodialysie quotidienne à domicile (HDQ)
Authors: Landi Vincent
Published: November 2020

Les besoins toujours croissants de la population en matière de soins est une réalité encore plus marquée lorsqu’il s’agit de prise en charge des patients atteints de maladies chroniques. L’INSEE [1] met clairement en évidence les difficultés auxquelles sera soumis le système de Santé Français si une réflexion sur les modalités de financement mais également sur la structure de l’offre de soins n’est pas menée dans les meilleurs délais.

Dans ce contexte de nécessaire évolution du système, il semble important que les acteurs de terrain puissent également apporter des éléments objectifs permettant d’alimenter une réflexion collective.

Cette note concerne la prise en charge des patients atteints de maladie rénale chronique. Elle a vocation à apporter des informations issues de l’expertise de professionnels du secteur destinées à objectiver quelques propositions détaillées dans sa dernière partie.
Impact of diuresis and number of exchanges on Continuous Ambulatory Peritoneal Dialysis related peritonitis risk in RDPLF registry.
Authors: Vachey Clément , Roubiou Caroline , Bresson-Vautrin Catherine , Courivaud Cécile
Published: November 2020

Introduction

Peritonitis is still a frequent complication among patients undergoing peritoneal dialysis (PD) and it’s associated with a significant morbimortality. The aim of our study was to investigate the impact of diuresis volume and number of exchanges (NE) on continuous ambulatory peritoneal dialysis (CAPD) related peritonitis risk.

Methods

This study was performed with data from the French peritoneal dialysis registry (RDPLF). We included every incident patient in the registry from January 2010 to November 2019 who had at least an adequacy evaluation. Peritonitis risk was assessed firstly by estimating a peritonitis rate per year undergoing PD and secondly by focusing on time to first peritonitis, taking into account competing risks (kidney transplantation, switch to hemodialysis, PD withdrawal whatever the cause or death). Patients whose diuresis was <500mL/24 hours were considered oliguric.

Results

We included 620 patients in our analysis. The mean age was 72,9 (standard deviation (SD)=15,1). Two hundred and six (39,55%) had at least one peritonitis episode. No difference was observed between oliguric patients and the others. However, we noticed an increased risk in patients with a NE≥22 per week (HR=1,55, P=0,0005 and HR=1,47, P=0,02 considering competing risks). We also observed a lower risk in diabetic patients HR=0,74, p=0,02 and HR=0,77, p=0,0497).

Conclusion

We didn’t find any impact of diuresis volume on peritonitis risk. Whereas, the NE seems to be a considerable risk factor, especially when it’s superior to 22 per week.
Situation of the Covid-19 epidemic in patients on peritoneal dialysis on 2020/05/15 in France : RDPLF data-base
Authors: Nouvier Mathilde , Verger Christian , Fouque Denis
Published: November 2020

Numerous studies have shown that chronic renal failure, whatever the treatment, is an important risk factor during the SARS-Cov2 pandemic. We present the incidence of COVID-19 infection, and its lethality, in France according to data from the French Language Peritoneal Dialysis Registry (RDPLF), during the period of the epidemic peak between March 1 and May 15, 2020.

Of the 3,104 patients treated with PD during this period, from 156 centers, 59 contracted COVID-19, ie 1.8%, a percentage significantly lower than that observed in center hemodialysis. Diabetes was found in 64% of infected patients while it was only present in 36% of uncontaminated patients. The mode of contamination was attributed to a hospital stay in 19% of the cases, a family infection in 17% of the cases, treatment in nursing homes in 15% of the cases, unknown in 44% of the cases. Sixty-two percent of the infected patients were on assisted PD, without identifying the source of contamination. The mortality rate was high at 40%, comparable to other countries in PD. A comparison with hemodialysis could only be made after adjustment for comorbidities and patient profiles: data on hemodialysis are not available in the RDPLF.

Home peritoneal dialysis decreases the risk of Covid-19 contamination, but associated comorbidities and age are the source of high mortality. Non-autonomous patients have a higher risk of contamination.
Revue de presse club des jeunes néphrologues (CJN) : 3e trimestre 2020 de la Dialyse à Domicile
Authors: Gosselin Morgane , Lanot Antoine , Lavainne Frédéric , Morinière Beaume Julie , Nodimar Céline , Seret Guillaume , Vrigneaud Laurence , Bataille Stanislas , Carceles Odette
Published: November 2020

Introduction (NDLR)

Un groupe de néphrologues issus du Club des jeunes Néphrologues sélectionne chaque mois à tour de rôle un article de la littérature internationale en rapport avec la dialyse à domicile, Dialyse Péritonéale ou Hémodialyse à Domicile, et en propose un résumé en français et son analyse. Ces résumés sont librement téléchargeables sur le site du RDPLF à l’adresse :https://www.rdplf.org/biblio.html. Depuis décembre 2018 ceux sélectionnés au cours du trimestre par ces néphrologues sont publiés sous leur nom dans la présente rubrique du Bulletin de la Dialyse à Domicile.
Accessing Chemo- and Regioselective Benzylic and Aromatic Oxidations by Protein Engineering of an Unspecific Peroxygenase
Authors: Knorrscheidt Anja , Soler Jordi , Hünecke Nicole , Püllmann Pascal , Garcia-Borràs Marc , Weissenborn Martin
Published: November 2020

Unspecific peroxygenases (UPOs) enable oxyfunctionalisations of a broad substrate range with unparalleled activities. Tailoring these enzymes for chemo- and regioselective transformations represents a grand challenge due to the difficulties in their heterologous productions. Herein, we performed a protein engineering in S. cerevisiae with the novel MthUPO. Experimental approaches were combined with computational modelling resulting in the screening of more than 5,300 transformants. This protein engineering led to a significant reshaping of the active site as elucidated by molecular dynamics. The kcat/Km was improved by 16.5-fold. Variants were identified with high chemo- and regioselectivities in the oxyfunctionalisation of aromatic and benzylic carbons, respectively. The benzylic hydroxylation was demonstrated to perform with excellent enantioselectivities of 95 % ee. Additionally, the first reported effective exchange of the conserved catalytic Glu residue was observed.
Piezoelectricity of a Ferrocene-based Organic Small Molecule
Authors: Feng Xing , Li Ling , Zhang Jianyu , Wang Xiaohui , Wang Qingsong , Gao Wei , Redshaw Carl , Huo Nengjie , Li Jingbo , Lam Jacky W. Y. , Tang Ben Zhong
Published: November 2020

Non-centrosymmetric crystals with piezoelectric properties have emerged as promising materials for smart wearable systems and biomimetic robots. Here we present a novel small ferrocene-based organic molecule crystal (Fc-Cz) possessing high anisotropic-dependent optical and electronic properties, which has been utilized as an ultrasensitive piezoelectric material for the development of a strain sensor. The flexible piezoelectric sensor can distinguish subtle strain or deformations (such as wrist motion) with fast response time (< 40 ms) via detectable piezoelectric signals (Imax = 580 pA). Density functional theory (DFT) indicated that the external pressure can affect the dipole moment by changing the molecular configuration of the asymmetric single crystal Fc-Cz in the crystalline state, leading to a change of polarity, as well as an enhanced dielectric constant. Based on our knowledge, this work is the first example verifying that artificial organic small molecules can serve as simple, stable, high-performance tactile sensors, and this has the potential to open the door to low-cost flexible wearable devices and energy harvesting applications.
The Role of Al3+-Based Aqueous Electrolytes in the Charge Storage Mechanism of MnOx Cathodes
Authors: Balland Véronique , Mateos Mickaël , Harris Kenneth D. , Limoges Benoit
Published: November 2020

Rechargeable aqueous aluminium batteries are the subject of growing interest, but the charge storage mechanisms at manganese oxide-based cathodes remain poorly understood with as many mechanisms as studies. Here, we use an original in situ spectroelectrochemical methodology to unambiguously demonstrate that the reversible proton-coupled MnO2-to-Mn2+ conversion is the main charge storage mechanism occurring at MnO2 cathodes over a range of slightly acidic Al3+-based aqueous electrolytes. In Zn/MnO2 assemblies, this mechanism is associated with high gravimetric capacity and discharge potentials, up to 560 mAh·g-1 and 1.76 V respectively, attractive efficiencies (CE > 98.5 % and EE > 80%) and excellent cyclability (> 750 cycles at 10 A·g-1). Finally, we conducted a critical analysis of the data previously published on MnOx cathodes in Al3+-based aqueous electrolytes to conclude on a universal charge storage mechanism, i.e., the reversible electrodissolution/electrodeposition of MnO2.
In Situ Generation of Radical Initiators: Air Stable Amine-Borane Complexes Promote Atom Transfer Radical Additions of Alkyl Halides to Alkenes
Authors: Liautard Virginie , Delgado Marine , Colin Boris , Chabaud Laurent , Michaud Guillaume , Pucheault Mathieu
Published: November 2020

Despite their instability, carbon-centered radicals have been involved as intermediates in many organic transformations that are essential to mankind. Radical polymerization,1-5 for example, provides a large part of polyacrylates and polystyrenes used as commodities in our everyday life. On a molecular level, the outcome of radical-mediated reactions is usually very different from those obtained via classical ionic based organic reactions. This is known since the 1930’s6 and has witnessed extensive developments such as radical cyclization or Atom Transfer Radical Additions (ATRA)7-9 . Radical processes10 typically begin with an initiation step in which the first radical species is created. It is eventually followed by one or several transfer steps which ultimately conduct to the formation of the main radical intermediate responsible for the reaction. The propagation steps of the radical chain are a succession of radical based transformations which, combined, outline the expected transformation.