bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Daya Saing Ekspor Rumput Laut Olahan Indonesia
Authors: Adiguna Aries Dwi , Krisnamurthi Bayu , Erwidodo Erwidodo
Published: June 2022

Indonesia is one of the main exporters of seaweed in the world market. Seaweed traded consists of raw products and processed products. Processed seaweed products are in the form of gelatin with HS code 130231 and carrageenan with HS code 130239. The high demand and value of processed seaweed products is an opportunity for Indonesia. The total world demand for agar in 2018 was 275,188,000. US$ increased 4.9 percent from the previous year, while carrageenan in 2018 had total world demand amounted to 1,131,820,000 US$, an increase of 8.4 percent from the previous year (ITC, 2019). This research aimed to analyze the competitiveness of processed seaweed and determine policies to increase Indonesian processed seaweed exports in the world market. The data in this study used secondary data. The competitiveness of processed Indonesian seaweed products is measured using Revealed Comparative Analysis (RCA) and Export Product Dynamic (EPD). The results of the analysis showed that processed Indonesian seaweed in the form of carrageenan and agar has comparative competitiveness in the world market. The position of competitiveness in the main destination countries with rising star status has strong competitiveness, but the increasing demand for processed seaweed in the world has caused Indonesia to lose the opportunity to optimize its profits. Indonesia needs to increase the quantity of processed seaweed exports in the form of carrageenan and agar by encouraging domestic investment to develop the seaweed processing industry sector.
Kinerja Keuangan Perusahaan Kakao Selama Pandemi Covid-19: Sebuah Pendekatan Economic Value Added (EVA)
Authors: Aviny Eva Yolynda
Published: June 2022

The Covid-19 pandemic brings around a multidimensional crisis that sway the world’s economy and believed to affect the performance of any industry. Cocoa processing industry, which is well known greatly contributes to Indonesia’s economy, is one of industry that is suspected has a significant effect to the performance of Indonesia’s economy. As one of export commodity, during the pandemic (2019-2020) the cocoa processing industry contributed for about USD 548 million to total Indonesia’s foreign exchange. This lead to a question: does the increase implies that the cocoa processing industry has a good financial performance? Therefore, this study aims to evaluate the financial performance of two cocoa processing company before and during Covid-19 pandemic by occupying the financial report of PT Bumi Teknokultura Unggul TBK and PT Wahana Interfood Nusantara from 2018 till 2020. The Economic Value Added approach was used to evaluate the financial performance of both company. The result of Economic Value Added (EVA) Analysis confirmed that this pandemic seem affecting the financial performance of the two company, which is both company’s performance tend to decrease. Yet, a different performance between the two was depicted as well by EVA, in which the EVA of PT Wahana Interfood Nusantara is positive while the PT Bumi Teknokultura Unggul Tbk’s is negative. This implies that PT Wahana Interfood Nusantara has a better financial performance than PT Bumi Teknokultura Unggul Tbk.
Penerapan Metode CART pada Pengklasifikasian Bekerja dan Pengangguran di Kabupaten Subang
Authors: Nabila Ilma , Sumertajaya I Made , Raharjo Mulianto
Published: June 2022

Pengangguran merupakan masalah kompleks yang dihadapi oleh negara berkembang salah satunya Indonesia. Tingginya angka pengangguran di Indonesia berdampak pada kemiskinan sehingga pemerintah berupaya untuk melakukan pembangunan ekonomi. Subang merupakan salah satu kabupaten yang menyumbang 8,68 persen tingkat pengangguran terbuka pada tahun 2019 dan meningkat sebesar 9,48 persen pada tahun 2020. Gencarnya pembangunan kawasan industri dan smart city di Subang menjadi salah satu upaya mengurangi pengangguran. Classification and regression tree (CART) adalah metode yang digunakan pada penelitian ini untuk mengetahui faktor-faktor yang berpengaruh terhadap pengangguran di Kabupaten Subang. Metode CART memiliki kelebihan, yaitu mudah menginterpretasikan terhadap hasil analisis. Nilai akurasi dari pohon klasifikasi yang dihasilkan cukup rendah disebabkan oleh adanya ketidakseimbangan data. Oleh karena itu, metode SMOTE menjadi metode yang digunakan untuk menangani masalah tersebut. Pohon klasifikasi optimal terbentuk dari 17 simpul terminal dan 6 peubah penjelas. 7 simpul terminal menggambarkan bekerja sebagai bekerja dan 10 simpul terminal menggambarkan pengangguran sebagai pengangguran. 6 peubah penjelas terdiri dari status perkawinan (X3), mengikuti pelatihan kerja (X5), status dalam keluarga (X4), tingkat pendidikan (X2), jenis kelamin (X1), dan usia (X6).
Analisis Tingkat Kepuasan Pelanggan dan Loyalitas Pelanggan terhadap Cafe Infinity Coffee
Authors: Prathama Muhammad Nuruddin , Aidi Muhammad Nur , Soleh Agus Mohamad
Published: June 2022

Bisnis cafe dan restoran merupakan salah satu bisnis paling kompetitif dan memiliki pasar yang cukup besar di Jakarta. Dalam hal ini pemilik restoran harus mengetahui keinginan serta preferensi dari pembelinya. Penelitian ini dilaksanakan di salah satu cafe di Jakarta “Infinity coffee”, penelitian ini dilakukan dengan mengidentifikasi karakteristik konsumen, kepuasan konsumen, serta loyalitas konsumen.  Menerapkan analisis kepuasan konsumen dalam bisnis Infinity coffee bisa meningkatkan pemahaman mengenai apa yang diinginkan konsumen Infinity coffee dan bisa meningkatkan kualitas pelayanan Infinity coffee berdasarkan hasil penelitian. Metode analisis yang digunakan pada penelitian ini yaitu analisis deskriptif, Important Performance Analysis (IPA), dan Consumer Satisfaction Index (CSI) serta analisis korespondensi. Hasil penelitian ini menunjukan seluruh indeks kepuasan pelayanan Infinity coffee untuk seluruh aspek berada diatas 80% yang berarti nilai tersebut masuk dalam kategori puas. Namun demikian, pada diagram pencar IPA terlihat bahwa terdapat atribut dengan tingkat kepentingan tinggi yang perlu ditingkatkan kualitas pelayanannya. Salah satu atribut terpenting yang jadi prioritas perbaikan adalah atribut kelengkapan sarana pendukung dan fasilitas fisik yang memadai. Metode yang digunakan terbukti cukup tepat dalam menguji tingkat kepuasan konsumen serta mengetahui lebih karakteristik konsumen.
Perbandingan Pengklasifikasian Metode Support Vector Machine dan Random Forest (Kasus Perusahaan Kebun Kelapa Sawit)
Authors: Achmad Nabila Destyana , Soleh Agus M , Rizki Akbar
Published: June 2022

Palm oil is one of the leading commodities that support the economy in Indonesia. One of the companies engaged in the oil palm plantation sector has 146 units of oil palm plantations. It is very important to optimize oil palm production, so it is necessary to classify the status of plantation units. Classification aims to predict new plantation units and find the most important variables in the modeling process. The variables used were the status of the garden as a response variable and nine explanatory variables, namely harvested area, rainfall, percentage of normal fruit, fresh fruit bunches production, oil palm loose fruits, production, harvest job performance, harvesting rotation, and farmers. The classification process is carried out using the Support Vector Machine and Random Forest methods to find which method is the best. The data is divided into 80% training data and 20% test data with ten iterations so that ten models are produced for each method. Comparing accuracy value, F1 score, and Area Under Curve (AUC) to evaluate the model. The modeling results show that the random forest method has better performance than the SVM method. The random forest has an average of accuracy, F1 score, and AUC, respectively, is 90%, 86%, and 89%. Variables of harvest job performance, oil palm loose fruits, harvested area, rainfall, and harvesting rotation are important variables that contribute more than 10% of the model. The results of the research are used for the evaluation and development process of oil palm companies by taking into account the result of important variables that affect productivity and predictive results of new plantation units
Analisis Regresi Data Panel Faktor-Faktor yang Memengaruhi Indeks Pembangunan Gender Perempuan di Jawa Barat
Authors: Shabrina Agatha Nur , Masjkur Mohammad , Soleh Agus M
Published: June 2022

The Gender Development Index (GDI) is a measure of human development based on the dimension of health, knowledge, and economic in a region by considering equality between women and men. West Java Province had the lowest women’s GDI in Java Island in 2017-2019. Those condition shows that there is still a gap in human development based on gender in West Java Province. The study intends to identify the factors affecting women’s GDI in West Java from 2017 to 2019 by applying a regression analysis method of panel data  involve 27 regions in West Java Province and determining the best model. Regression analysis of panel data is an alternative method to estimate parameter which is a combination of time series and cross section data. The method used were data exploration, regression analysis of panel data, and classical assumption tests. The results showed that there was an individual effect on the model. The best model was a fixed effect model with individual effects. This model produces a coefficient of determination wih an R2 value of 77,75%. This suggest that the model is good enough to illustrate that 22,25% of the varience of women’s GDI at the region level in West Java can not be explained by the model. Variable that have impact to women’s GDI are the percentage of the population who experience health complaints, the percentage of population who have health insurance for hospitalization, women's income contributions, and school participation rates at the Junior High School or the same level.
Aflatoxin and molecular characterization of isolates from stored food in some selected Local Government in Ekiti State
Authors: Fajilade OT  , Oyelade WA
Published: June 2022

Aflatoxins are secondary metabolites produced by fungi species from the genus Aspergillus, notably A. flavus, A. parasiticus and A. nomius, which develop naturally in food products and tend to cause wide array of toxic effects in several animal species, including humans. Aflatoxins are typically reported in dry food commodities such as cereals, spices, and dry fruits. This research was conducted in Ido-Osi, Efon, and Emure Local Government Areas in Ekiti State, Nigeria. Three samples; Local rice (Oryza sativa L), Maize (Zea mays) and Groundnut (Arachis hypogaea L) were obtained from three popular markets from each local government. About 10 grains of each sample were selected at random into separate petri plates and surface sterilized in 1% hypochlorite for 3 minutes. The grains were rinsed several times with distilled water. The organisms associated with the samples were isolated using Direct plating method while the aflatoxin content was determined using ELIZA. The result showed the occurrence of five (5) different fungal species distributed among the stored foods. These include: Aspergillus spp., Rhizopus spp. Penicillium spp. Mucor spp. and Botrytis spp. The species determination was confirmed by PCR with the primers specific for the ITS-rDNA region sequencing. The organisms were confirmed to the species level to be; Aspergillus flavus and Trichoderma viridie. Aflatoxin B1 was present in all the samples in which sample 6 has the highest values of 240ppb while aflatoxin B2 was relatively low. The prevalence of Aspergillus flavus in the stored foods is above 50%, which is very high and indicate the possible highlevel production of aflatoxin in food samples under study.
Grid interface of solar PV transformer-less inverters using enhanced phase locked loop
Authors: Janardhan Gurram  , NNV Surendra Babu Rudy  , GN Srinivas
Published: June 2022

The era of Renewable Energy sources has overtaken the usage of conventional energy sources like coal, fossil fuels etc. This paper presents grid interface of single phase transformerless inverter system using enhanced phase locked loop. To connect solar PV with grid it is required to estimate phase angle, voltage amplitude and phase angle measurement of grid and the output of inverter. It is required to track exact utility voltage vector for reliable control of power to the grid. This paper compares the frequency measurement and phase angle and voltage amplitude using simple phase locked loop system along with enhanced phase locked loop so that the enhanced phase locked loop (EPLL) is proved to be giving superior performance over simple phase locked loop. The simulation of both the techniques are pursued and the results are compared.
Role of stem cell in medical care and treatment
Authors: Faizan Khan  , Kailash Sahu  , Saman Pathan  , Bhaskar Kumar Gupta
Published: June 2022

In stem cell transplants, stem cells replace cells damaged by serve as a way for the donor's immune system to fight some types of cancer and blood-related diseases, such as leukemia, lymphoma, neuroblastoma and multiple myeloma. This research has the potential to affect the lives of Millions of people by offering unprecedented opportunities for developing new medical Therapies for debilitating diseases and a new way to explore fundamental questions of Biology. Stem cells can be guided into becoming specific cells that can be used in people to regenerate and repair tissues that have been damaged or affected by disease. People who might benefit from stem cell therapies include those with spinal cord injuries, type -1 diabetes, Parkinson's disease, amyotrophic lateral sclerosis, Alzheimer's disease, heart disease, stroke, burns, cancer and osteoarthritis. Stem cells may have the potential to be grown to become new tissue for use in transplant and regenerative medicine. Researchers continue to advance the knowledge on stem cells and their applications in transplant and regenerative medicine. Specialized types of stem cells have the ability to stop immune responses. Stem cells may therefore be very useful as a therapy for diseases in which organs are damaged or where the immune system is too active. Some types of stem cells are already used for therapy, such as the hematopoietic (blood) stem cells, which are used for the treatment of bone marrow cancer.
Urinary schistosomiasis prevalence and risk factors among school children at matta-barrage in the Tikar Plain of Magba, West Region, Cameroon: A situational analysis in rural area
Authors: Gilbert Gautier Bong Bong  , Véronique Metiendjo  , Ghislaine Haverie Ateba Mimfoumou  , Christian Taheu Ngounouh  , Philippe Salomon Nguwoh
Published: June 2022

Background: Urinary schistosomiasis (US), caused by Schistosoma haematobium (S. haematobium) has reported very high in Cameroon and children were more infected. The study was conducted to assess the prevalence of US and risk factors among school children at Matta-Barrage in Magba sub-division. Methods: A cross-sectional study was conducted during one month period from May to June, 2019 among pupils at Matta-Barrage public school after parental consent. The sample was taken after physical exercise and the urine was collected at the end of micturition in a sterile urine container. Data collected were performed using SPSS version 25.0 and P-value ˂ 0.05 was considered statistically significant. Results: Of the 300 pupils enrolled, the overall prevalence of US was 43.0% and among children infested, 82.17% (106/129) had hematuria and 58.13% (75/129) had dysuria. The positivity rate of S. haematobium was higher in boys (69.0%; 89/129) versus (vs.) 31.0% (40/129) for the girls (P=0.03) and the children aged between 8-10 years were more infested (44.78%; 57/129), P=0.38. Regarding the risk factors, the multivariate analysis shows that sources of washing, sources of cooking/drinking water, and ethnic groups such as Arabe, Kotoko, Fulbe and Tikar were statistically associated with S. haematobium infestation (P˂ 0.05). Conclusion: The prevalence of US remain high among school children at Matta-Barrage in Magba sub-division. Meanwhile, the boys and childen aged 8-10 years were more infested. The risk factors such as sources of washing, sources of cooking/drinking water, and ethnic groups such as Arabe, Kotoko, Fulbe, and Tikar were statistically associated with S. haematobium infestation.
Study of the physio-chemical parameters for testing water: A review
Authors: Dhaswadikar Usha Sitaram
Published: June 2022

Due to undesired changes in the physical, chemical and biological characteristics of air, water and soil people on global scale are under threat. Due to increased human population, industrialization, use of fertilizers and man-made activity water become highly polluted. Hence the quality of drinking water should be checked at regular time of interval, because due to use of contaminated water, human population suffers from various water brone diseases, like cholera, hepatitis, dysentery, typhoid etc. The good quality of water prevents many diseases and saves the life of human beings. Hence it is necessary to know about different physic-chemical properties such as colour, temp, acidity, hardness, PH, DO, BOD, alkalinity, sulphate, chloride used for testing the water quality. Some water analysis reports with physic chemical parameters have been given for the exploring parameter study guidelines of different physic chemical parameters also have been given for comparing the value of real water sample.
The effect of taxpayer awareness, taxpayer numbers, tax billing, and taxpayer compliance to tax revenue
Authors: Fransiskus X. Seran  , Pompong Budi Setiadi  , Sri Rahayu
Published: June 2022

The excavation of the potential of domestic revenues will continue to be improved optimally through the expansion of non-oil and gas revenue sources, in order to replace the funding of countries sourced from external debt. One of the dominant sources of domestic income is tax revenue. Tax revenue is a description of public participation in financing the implementation of government and state development. Given that the tax revenue will not be able to increase without the participation of the taxpayer itself. The purpose of this study is to analyze the influence of awareness of taxpayers, NPWP ownership, tax collection, and taxpayer compliance with tax revenue. Samples in this study are taxpayers of individuals registered in KPP Pratama Surabaya Wonocolo, Indonesia with sampling technique that is Convenience sampling. The data used are primary data collected through answers to each given statement. The method of analysis using multiple linear regression. The result of this research is the awareness of the taxpayer has an influence on the tax revenue, while the ownership of NPWP, tax collection, and taxpayer compliance has no effect on tax revenue.
Review on Green Synthesis of Nanoparticles using Various Strong Electrolytic Metal Solutions Mediated by Various Plant Parts
Authors: Vanitha Gothandapani , Manikandan Ramalingam , Sathiyamoorthi Kaliyan , Dhinakaran Boorasamy
Published: June 2022

The present review targets the comparative biogenic synthesis and mechanisms of nanoparticles using various plant-parts extracts and various solutions of strong electrolytic metal salts. The metal salts are AgNO3, Zn(OAc)2, Zn(NO3)2, ZnSO4, CuSO4.5H2O, Cu(NO3)2, Cu(OAc)2, CuCl2, HAuCl4.3H2O, Mg(NO3)2, Mg(OAc)2, TiO(OH)2, Fe(NO3)2.6H2O, FeCl3.6H2O, FeCl2.4H2O, Al(NO3)2, BaCl2·2H2O, Bi(NO3)2, PdCl2, H2PtCl6.6H2O, NaHSeO3, Na2SeO3, Ca(NO3)2.4H2O, ZrOCl2.4H2O, Zr(OAc)4, NiCl2, NiSO4.6H2O, Ni(NO3)2, VCl2, Co(NO3)2 6H2O, CoCl2, Hg(OAc)2, Mn(OAc)2, (NH4)6Mo7O24, Sr(NO3)2, SnCl2.2H2O, Na2S2O3.5H2O, K2Cr2O7, Cr(NO)3.9H2O and Pb(NO3)2. These salts are soluble to produce to highly active positive metal ions in deionised water. These ions are effectively nucleated in the plant constituents. The plant acts akin to a huge ‘‘bio-laboratory” comprising of leaves, seeds, steam, root, sprout, fruits, latex, parks, fruits peel, fruits, juices. etc… which are composed of biomolecules and phytoconstituents. These naturally happening biomolecules and phytoconstituents have been recognized to play an energetic role in the formation of nanoparticles with discrete shapes and sizes thus acting as a pouring force for the manipulative of greener, safe and environmentally benign protocols for the synthesis of nanoparticles.
Efficiency of Bovine Embryo Transfer in JSC "Agroindustrial Firm "Nasha Zhitnitsa"
Authors: Knurov D.A. , Brigida A.V. , Ivanova D.V. , Ignatiev A.V.
Published: June 2022

The aim of the study was to estimate the effectiveness of embryo survival rate in cows and heifers selected as recipients. Embryo transfer was carried out in JSC "Agroindustrial Firm "Nasha Zhitnitsa", Rodomanovo village, Smolensk region. The embryos were obtained from donors of the Aberdeen-Angus breed located in the Center for Reproductive Technologies LLC, Syreyka, Samara region. For the experiment, mongrel animals (n = 110) were selected and divided into two groups. The group I contained cows (n = 52) with 2-4 calves. Heifers (n = 58) of a random age were put into the group II. All the animals selected for the experiment were previously subjected to hormonal stimulation in order to synchronize their estrus cycles. The synchronization scheme included the introduction of an introvaginal Y-shaped implant "CIDR", intramuscular administration of GnRH analogues at a dose of 10 ml per animal and PgF2? cloprostenol at a dose of 500 mcg per animal. On the 7th day of the estrus cycle, all animals were transferred frozen-thawed embryos into the lateral horn of the uterus relative to the corpus luteum diagnosed on the ovary. Pregnancy diagnosis was performed on the 25th day after embryo transfer, which corresponded to the 32nd day of embryo development. During rectal examination of the cow recipients in group I, as for the result of the survival rate of transferred frozen-thawed embryos, pregnancy on the 30th day was diagnosed in 53.9% (28/52) of animals. In group II, in which heifers of random age were selected as recipients, pregnancy was diagnosed in 62.1% (36/58) of animals. Thus, the research results show that the survival rate of embryos in group II was significantly (P < 0.01) higher by 8.2% compared with the results obtained on animals in group I.
Evaluation of therapeutic efficacy of Tulatrin drug in infectious keratoconjunctivitis in cattle
Authors: Koba I.S. , Kozlov Yu.V. , Reshetnikova D.G.
Published: June 2022

Infectious keratoconjunctivitis, or moraxellosis, is a highly contagious infectious disease of the eyes of cattle, characterized by conjunctivitis and ulcerative keratitis, found throughout the world. In this regard, the development of new means and treatment schemes is an urgent area in modern practical veterinary science. The aim of the work was to evaluate the therapeutic effectiveness of the drug Tulatrin in infectious keratoconjunctivitis in cattle caused by Moraxella bovis Tulatrin is a drug developed by NITA-PHARM, which belongs to the group of antibacterial agents of macrolides. Active substance of the drug Tulatromycin -- has a wide spectrum of action against many gram-positive and gram-negative microorganisms, including Mannheimia (Pasteurella) haemolytica, Pasteurella multocida, Haemophilus somnus, Haemophilus parasuis, Mycoplasma hyopneumoniae, Moraxella bovis, Neisseria spp.Analyzing anamnestic data, we found that in most cases calves developed one-sided keratoconjunctivitis, which was characterized by the following clinical signs: first, abundant lacrimation appeared, the outflows were first transparent, then they thickened and took on a purulent character, the mucosa acquired a fine-grained structure.After the therapy, the following changes were noted in the clinical picture: in the experimental group, the release of purulent exudate decreased in animals on day 2-3. By day 3-4, a decrease in swelling and conjunctival hyperemia was noted, lacrimal fluid acquired transparency. On day 4-6, the absence of lacrimation, and inflammation of the conjunctiva, as well as lightening of the focus of corneal haze were recorded.The therapeutic efficacy of the drug Tulatrin in infectious keratoconjunctivitis in cattle caused - Moraxella bovis was 100%. The use of the Draksin analogue drug in the same pathology made it possible to obtain a similar therapeutic effect - 100%.When using the drug Tulatrin, the recovery of animals occurred on average on the 5th day. The recovery time for animals using Draksin was 6.3 days.
Reduced sanitary quality of milk in patients with metrite cows after childbirth
Authors: Loshchinin S.O. , Firsov G.M. , Filatova A.V. , Akhmadov V.T. , Avdeenko V.S. , Aygubova S.A.
Published: June 2022

t has been established that the development of purulent-catharal inflammation of the former gravid horn of the uterus in cows after delivery as a result of abortion, dystocia, stillbirth, caesarean section, uterine dislocation or seedlings is accompanied by an increased microbial and fungal background of the uterus, in their symbiotic relationships. In cows without delivery during childbirth, only from the 5... 9th day after calving in 35.37% cows, the genitals were contaminated with various non-pathogenic microflora. In cows given maternity care, already on the 3rd day after calving, bacteria were isolated, which in 74.5% of cases were contaminated with pathogenic microflora S. aureus in 15.5% of cases, E. coli in 37%, K. pneumonia - 12% and S. pyogenes in 10% of cases. The results of mycological studies revealed that A. fumigatus, C. albicans and C. crusei were isolated from cows as a result of childbirth. It was found that in cows with purulent-catharal inflammation of the uterus in the milk of sick cows, a significant change, with a high degree of correlation, is: the content of somatic cells (CK) r = 0.63, lactoperoxidase (LPO) r = 0.65 and lactoferin (LF) r = 0.66. Milk obtained from patients with purulent-catharal inflammation of the former gravid horn of the uterus showed 2 times the total bacterial insemination than milk obtained from clinically healthy animals. At the same time it has a reduced number of lactic acid organisms after the first days of storage. Acid formation occurs faster by 5.0-15.0% than in control samples of milk prepared to produce lactic acid products. Acidity in Lactobacillus bulgarus-fermented milk was 12.0-13.3% higher than in starter control samples, and the viability of symbiotic coupling cells was an order of magnitude lower (2.5x106 vs. 2.5x107) compared to lactic acid control samples.
Application of IHC method for diagnosis of CSF
Authors: Stafford V.V. , Alekseev K.P. , Yuzhakov A.G. , Gulyukin A.M. , Aliper T.I.
Published: June 2022

The article presents experimental data on the reproduction of classical swine fever and the development of immunohistochemical method for the study of the obtained pathological material. 2 groups of animals were involved in the experiment (experimental infection group and non-control). During the experiment, blood was taken to obtain serum for further study, as well as organs for histological (paraffin embedded samples) examination and observation of organ pathology and native samples (frozen slices) for testing them in an immunohistochemical reaction. For indirect immunohistochemical reaction, primary mouse monoclonal antibodies specific to the recombinant protein E2 of the classical swine fever virus and secondary goat antibodies to mouse immunoglobulin labeled with horseradish peroxidase were used. Histological examination of the palatine tonsil, kidneys, spleen, liver, lung, lymph nodes and spinal cord brain revealed the development of pathological in all samples from the experimental infection group. The use of the ELISA method confirmed seroconversion of inoculated animals from the experimental infection group by the end of the study (DPI 14). Also, PCR studies have shown viremia in the same animals starting from the 2nd day of the experiment and up to the date of euthanasia of the animals at the end of the experiment, which corresponds to 14 days after infection. Despite the pathological changes in all the organs we studied, the indirect immunohistochemical method showed a positive result only in the spleen and in the palatine tonsil, while the number of positive sites was greater in the spleen. The nature of the chromogen distribution indicated the presence of the CSF virus antigen in the red pulp of the spleen, in leukocyte cells. The data obtained by us in the immunohistochemical reaction correlate with the data obtained in ELISA and PCR in the study of blood sera.
Metabolism of cows when feeding premixes based on forest biomass and natural minerals
Authors: Tereshchenko V.A. , Ivanov E.A. , Lyubimova Yu.G. , Ivanova O.V. , Kicheeva A.G.
Published: June 2022

Natural plant and mineral renewable resources are a constant source of a complex of nutrients and biologically active substances necessary for the animal organism. In order to balance diets, it is important to use forest biomass and natural minerals in feeding farm animals. Studies on the influence of premixes based on forest resources (pine needles, pine nut shells, arabinogalactan) and natural minerals (bentonite clay, expanded vermiculite) on the metabolism of lactating cows were conducted in the EPF "Mikhailovskoye" branch of the FRC KSC SB RAS of the Krasnoyarsk region. According to the principle of analogues, 3 groups of Black-Motley breed dairy cows were formed at the age of the third calving, with a live weight of 530-550 kg (control and two experimental, 10 heads each). For 60 days, the control group of cows was fed the main diet, the cows of the 1st experimental group were fed coniferous flour (50 g/head/day), pine nut shell (50 g/head/day), arabinogalactan (5 g/head/day), vitamin and mineral complex (10 g/head/day) and bentonite clay (100 g/head/day) in addition to the main diet; 2nd the experimental group - coniferous flour (g/head/day), pine nut shell (g/head/day), arabinogalactan (g/head/day), vitamin and mineral complex (g/head/day), expanded vermiculite (g/head/day). The cows were kept in a tethered-walking way in the same conditions corresponding to the technology of dairy cattle breeding. Research and data processing were carried out according to generally accepted methods using modern equipment. As a result of the studies, it was determined that feeding the studied premixes does not have a negative effect on the metabolism of cows, all the studied blood and urine parameters corresponded to the norm for healthy animals. The most favorable biological effect on the body of cows was provided by feeding premix, which included expanded vermiculite (2nd experimental group), increasing the blood content of total protein by 14.4%, calcium by 23.8%, phosphorus by 16.8%, iron by 14.6% and contributing to an increase in the concentration of vitamin C in the blood by 6.0%.
Meat productivity of broiler chickens depending on the conditions of keeping and feeding when using microbial additives in the diet
Authors: Boyko A.A. , Koschaev A.G. , Lysenko Yu.A. , Shantyz A.Kh. , Luneva A.V. , Kopyltsov S.V.
Published: June 2022

The publication presents the results of the study of the influence of conditions of keeping and feeding with the use of a microbial feed additive in the diet of broiler chickens of the Ross 308 cross. The birds were kept both in the outdoor way and in cages. The additive is a lyophilized mass of beneficial microorganisms (Bifidobacterium lactis, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus) and vegetable fillers (edible citrus fiber and maltodextrin). The conducted scientific, production and laboratory studies have demonstrated that the conditions of broiler chickens did not have a significant impact on the studied parameters, however, the use of probiotics in the poultry diet contributed to the improvement of the safety, live weight and meat productivity of the cross. It was found that the safety of the experimental poultry contained by outdoor and cage methods, which additionally received the studied microbial additive in the diet, increased compared to similar groups, but without additives by 4.0 and 6.0 %, the live weight of broiler chickens on the last day of the experiment was 5.5 and 5.6 % more, there was also a decrease in feed costs by 1.0 kg of poultry growth by 3.9 and 4.4 %. The use of microbial additive contributed to an increase in the slaughter yield of the gutted carcass of experimental groups of broiler chickens by 3.2-4.0 % with statistically significant differences in the mass of the body parts of experimental birds compared with control groups.
The efficiency of obtaining twins by replanting embryos in preinseminated animals
Authors: Brigida A.V.
Published: June 2022

The purpose of our work was to determine the effectiveness of using of dairy cows as recipients for obtaining twin calves of the meat direction of produc-tivity. The study was conducted in LLC "Agricultural enterprise" Nikolaevskoe", Lugovets village, Mglinsky district. For the experiment cows and first-calf heifers (n=50) were selected and divided into three groups according to the breed princi-ple. In group I (n=22) Swiss breed animals were selected, in group II (n=13) - black-and-white cows, in group III (n=15) - crossbred cattle. All animals selected for the experiment were previously subjected to hormonal stimulation in order to synchronize the sexual cycles. The synchronization scheme included the injection of GnRh analogues at a dose of 10 ml IM and PgF2a cloprostenol at a dose of 500 mg per animal. With the manifestation of symptoms of estrus, all animals were arti-ficially inseminated with the sexed semen of a Belgian blue bull. On the seventh day of the sexual cycle, all inseminated animals had the procedure of replanting a frozen-thawed embryo into the contralateral uterine horn relative to the corpus lu-teum diagnosed on the ovary. To avoid freemartinism of heifers, the recipients were implanted with sexed embryos which were previously obtained from donor cows of the Hereford breed. During the study, it was found that the recipients in groups I, II and III had twins in 45.46% (10/22), 15.39% (2/13) and 20.0% (3/15) cases of joint application of biotechnological methods. The birth of one transplant calf was recorded in group I in 18.18% (4/22) of cows, in group II - 23.08% (3/13) and in group III - 33.33% (5/15) respectively. The birth of one calf obtained from artificial insemination was noted in group I in 31.82% (7/22) of animals, in group II - 38.46% (5/13) and in group III - 40.0% (6/15) respectively. The rest of the cows had no calves. Thus, it was found that the percentage of twins in the progeny was significantly higher (P?0.01) in group I compared with groups II and III. In groups I, II and III the total yield of calves was 140.92% (31/22), 92.32% (12/13) and 113.33% (17/15), respectively.
Analysis and Forecasting of the Climate Change Impact on the Traditional Industry of the Arctic North
Authors: Vladimirov L.N. , Machakhtyrov G.N. , Machakhtyrova V.A. , Slepsov E.S. , Shadrina Y.L.
Published: June 2022

The currently observed climate change towards warming affects the expansion of the suitable range of new species of insects and microorganisms for the northern region, which, in turn, being carriers of many diseases, can increase the risk of invasion of domestic animals that have not previously been found in these areas of diseases. It is easy to assume that any climate change can affect the established way of life of the indigenous peoples of the North, the main life-supporting occupation for which is still traditional farming - breeding reindeer, cattle, including aboriginal, and herd horses, as well as hunting, fishing, picking berries and herbs. At the same time, northern animal husbandry occupies an important place in the economy, due to the characteristic way of life of the indigenous peoples of the North, especially northern reindeer husbandry. The trend of change in the indicator of the average annual temperature from 1961 to 2020 has been established in Yakutia towards warming in both central and northern regions. Such changes in the considered climatic indicators have a significant impact on the expansion of the distribution area of the ixodidae (hard ticks) to previously inaccessible northern territories. Since 2000, in the territory of the Republic of Sakha (Yakutia), there has been a gradual increase of tick attacks cases on people with the annual coverage of new regions, including the Arctic. Although Yakutia is not endemic for tick-borne encephalitis, there is concern about the annual growth and expansion of the geography of tick attacks on the northern territories - if cases were previously noted only in 3 southern regions, then at present - in 21 regions, including Verkhoyansk, which is part of the Arctic group of regions. The correlation between these indicators was = 0.584. In the situation under study, in 34.14% of the total variability, the increase in the number of registered cases of tick bites is explained by changes in the average annual air temperature in certain areas.
The use of monoclonal antibodies to assess the B-cell immunity of cattle, sheep and goats
Authors: Ezdakova I.Yu. , Kapustina O.V. , Valtsiferova S.V. , Grigoriev A.G.
Published: June 2022

Currently, the use of monoclonal antibodies to study the immune status of animals is the most common in both medicine and veterinary medicine. Monoclonal antibodies were obtained by standard methods using a 2-fold intra-splenic injection of Ig with an interval of 14 days. As a result, an oligoclonal system of monoclonal antibodies to conformational epitopes of cattle IgM was created, specifically interacting with the antigenic determinants of the original, membrane-bound form of IgM, which is the basis of the antigenic B-cell receptor (BCR). B cells are an important structural component of the immune system. The process of antibody formation depends on the number and functional state of B cells. Early detection of target cells, which may be lymphocytes or their subpopulations, is important in infectious diseases. Monomeric IgM pentamer molecules are anchored in the B cell membrane and serve as the basis of the antigenic receptor. In the experiment, the method of immunoperoxidase staining was used, which is based on the detection and determination of the degree of intensity of membrane (sIgM) and cytoplasmic IgM (cIgM) staining. The number of B-lymphocytes in the blood of cattle (25.3±2.6%), sheep (32.0± 2.2%) and goats (20.0±1.5%) was determined. In the blood of calves aged 1 month, the number of B-lymphocytes is 19.2 ± 0.8%. Also, cells with the cytoplasmic form (cIgM) of class M immunoglobulin were found in the blood of calves. cIgM cells can be pre-B cells with cytoplasmic ?-chains or plasma cells synthesizing a soluble form of IgM. It was found that the main form of localization of surface IgM-receptors on B cells of cattle and sheep is an uneven distribution of sIgM along the perimeter of the cell, namely in the form of "patch-endocytosis", which is 68.9±3.9% and 84.0±4.2%, respectively; expression of sIgM-receptors on B lymphocytes of cattle livestock is 8.0high ± 1.0% and 10.5low ± 1.5%, and sheep - 6.3high ± 0.9% and 13.3low ± 0.6%. Thus, in healthy animals, the sIgM-receptors of B lymphocytes are distributed unevenly and with low density in the membrane.
Biological properties of cow colostrum
Authors: Ermilova T.S. , Soshnikov N.M. , Zakharkina N.I. , Kasharnaya O.V. , Ogarkova Zh.V.
Published: June 2022

Colostrum (beastings) is a specific secret of the mammary gland, preceding milk formation, having a unique composition and high nutritive value. Nowadays, no biologically active supplement or drug can replace it. This article presents a review of literature data of domestic and foreign authors on the composition and main biological properties of cow colostrum. Viability of young animals directly depends on colostrum quality and compliance with the rules of its feeding. The main biological properties of colostrum are primarily due to the content of active immune components in it, which are necessary for the formation of colostral (passive) immunity in calves and further formation of their immune system. During the first days of life, colostrum is necessary to protect the newborn from pathogenic microflora. The review paper discusses the mechanisms of passive immunity in calves, which are formed with involvement of immune proteins and enzymes of mammary gland secretion. In addition, colostrum contributes to the formation of the digestive, hematopoietic and endocrine systems. In connection with named functions, we reviewed the available data on the basic components of colostrum, vitamin and mineral composition, and acidity. The variability of indicators is discussed depending on the number of lactation, the transition to the production of mature milk, and other factors.
Benzthiophenes containing a 2-amino-ethyl fragment: synthesis, antibacterial, antiprotozoal and fungistatic activity
Authors: Chekrysheva V.V. , Urban G.A. , Zubenko A.A. , Fetisov L.N. , Svyatogorova A.E. , Kononenko K.N. , Klimenko A.I.
Published: June 2022

Benzthiophenes containing a 2-aminoethyl fragment were synthesized, at the NCRSRVI branch of FSBSC Federal Rostov Agrarian Scientific Center, and their antibacterial, antiprotozoal, and fungistatic activities were studied. We synthesized compounds based on 1-(2-chloroaryl)-3,4-dihydroisoquinolines by base-catalyzed nucleophilic substitution of an atom for a mercapto group containing an electron-withdrawing substituent, followed by recycling to the corresponding benzthiophenes, developed by us for the first time. Fifteen benzthiophene derivatives containing a 2-aminoethyl fragment have been synthesized. The starting compounds for the synthesis of benzothiophenes (compounds 1-6) showed a relatively weak activity against all the cultures studied. Only hydrazide 5 in terms of antiprotozoal activity corresponds to the level of chloroquine and is 4 times more active than amprolium. The formation of a benzothiophene ring significantly changes the activity of the compounds. Thus, compounds 14 and 15 (arylurea derivatives) have a noticeable fungistatic activity, although all other structures presented in this study are completely inactive against Penicillium italicum. It is also necessary to note the high antibacterial activity of compounds 14 and 15 against both gram-positive and gram-negative bacteria, which allows us to conclude that the search for active structures in the series of urea analogues 14 and 15 is promising. The azabenzthiophene derivative 11 showed the highest antiprotozoal activity, exceeding the indicator of chloroquine by 2 times and that of amprolium by 6 times. It should be assumed that the synthesis of analogues of compound 11 with other heterocyclic residues instead of the benzimidazole core (pyridine, imidazole, quinoxaline, pyrimidine) can lead to an increase in both antiprotozoal and (for the corresponding ureas) antibacterial activity. Analysis of the results of studying the biological activity of new compounds also showed that a high level of protistocidal activity (31.25-7.8 ?g/ml) was found in three compounds of the benzthiophene series: 5, 8, 11; average level (125-62.5 mcg/ml) - in five substances: 1, 4, 10, 14, 15. The antibacterial activity of the studied benzthiophene derivatives was manifested to a greater extent in relation to Gram-positive bacteria: twelve substances inhibited the growth of St. aureus All-Russian Collection of Microorganisms B-128. Five compounds were active against Gram-negative bacteria (E. coli All-Russian Collection of Microorganisms B-820). Combined antiprotozoal and antibacterial activity was found in compounds 1, 4, 5, 9, 10, 11, 14 and 15.
Examination of Obesity Awareness Levels of University Students in Terms of Different Variables
Authors:   Examination of Obesity Awareness Levels of University Students in Terms of Different Variables, Tozoğlu Burak , Polat Şeyda , Celık Hüsniye , Mazlumoglu Bahadır
Published: June 2022

The purpose of this study is to examine the obesity awareness levels of university students in terms of sport and different variables. The research was carried out on a total of 310 students, 107 male and 203 female, studying at Erzurum Atatürk University Kazım Karabekir Faculty of Education in the 2021/2022 academic year. Research data were collected by the obesity awareness scale developed by Allen (2011) and adapted into Turkish by Kafkas and Özen (2014) and a demographic information form prepared by the researchers. Frequency distribution was used to determine the demographic characteristics of the students. “T-test for Independent Samples” was used to examine the differentiation between two independent variables and obesity awareness scale sub-dimensions, "One-Way Analysis of Variance" was used to examine the differentiation between more than two variables and obesity awareness scale sub-dimensions, Pearson correlation analyzes were applied to determine the relationship between students' ages and obesity awareness scale sub-dimensions. All these tests were analyzed in the SPSS 21 package program and the significance level was taken as p<.05. As a result of the analyzes, it was determined that there was a significant difference between the students' personal monthly income and sports activity status and the obesity awareness scale sub-dimensions. It was found that the obesity awareness levels of the students who do sports activities are higher than those who do not.
Present situation of agricultural information needs and accessibility of women farmers in Imbulpe DS division in Sri Lanka
Authors: RATHNACHANDRA Dilini , MALKANTHİ Pushpa , SİVASHANKAR Padmanadan
Published: June 2022

Agricultural information and accessibility of women farmers are more crucial for enhancing food production. Thus, the main objectives of this research were to study the present situation of the agricultural information needs and accessibility of the women farmers in Imbulpe Divisional Secretariate (DS) Division of the country. Out of the women farmers of the study area, 238 were selected from the simple random sampling method as the sample for the study. A pre-tested, interviewer-administered questionnaire survey was used as the primary data collection method from March to July 2019. Descriptive statistics and chi-square analysis were used as the data analyzing methods. Based on the findings, women farmers showed that, they need more information regarding improved crop varieties, application of agrochemicals, new cropping systems and irrigation systems. Furthermore, most of the respondents showed that the higher level of accessibility to agricultural information on improved crop varieties. However, a lower level of agricultural information accessibility on suitable storage facilities was observed. Extension agents and successful women farmers act as their major sources of agricultural information. Furthermore, ICT equipment act as the least important agricultural information source. Age, marital status, educational level and monthly income were shown as the considerably higher associatiative variables with the agricultural information accessibility than the size of the farmland and farming experience of them. However, farmland size represented a moderate level of statistically significant positive association with the accessibility of agricultural information for the women farmers. Therefore, agricultural information accessibility can be enhanced by organizing awareness programs and extension service for the women farmers. Moreover, enhancement of accessibility for market information through the ICTs and encouragement of women farmers’ participation in the farming societies of Imbulpe area will be very important to develop the agricultural information accessibility furthermore.