bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrical Resistivity Tomography (ERT) for investigating subsurface structures in the karst area of the Ek Leuntie Cave, Aceh Province
Authors: Khatimah Husnul , Ismail Nazli , Kurniawan Fikry , Muksin Muksin , Nanda Muhammad , Asyqari Amir , Pramana Agus Hari , Afrizal Tomi , Putri Deviyani Rusdiyanti , Munir Badrul , Azmi Nurul
Published: June 2024

Ek Leuntie Cave, in the southern city of Banda Aceh, Aceh Province, is recognized as an educational media based on geopark sites because it holds a 7400-year paleotsunami track record. As a karst area, Ek Leuntie Cave is vulnerable to the impact of geological disasters such as earthquakes, sinkholes and subsidence. As an effort to reduce the negative impact of geological disasters, geophysical studies have been applied in the Ek Leuntie Cave Karst area. The study aims to identify the subsurface geological structure of the Karst area. The identification was carried out using the Electrical Resistivity Tomography (ERT) method of Wenner-Schlumberger configuration applied to two measurement trajectories that have South-North and West-East directions in front of the Ek Leuntie Cave door. ERT data acquisition results were then modeled in inversion using Res2Dinv software to produce a 2D model of the subsurface geological structure of the Ek Leuntie Cave karst area based on variations in rock resistivity values. Interpretation of subsurface geological structure modeling is validated with shallow drilling data (Hand Auger). The inversion modeling results show that the rock resistivity value in the Ek Leuntie Cave area reaches a depth of 30 m which has a resistivity value of 0 - 1000 Ωm. The results of the interpretation of resistivity values and validation of drill data show that the Ek Leuntie Cave area is covered by a layer of sand with fine to coarse texture and the layer below is a layer of limestone at a depth of ± 0.4 meters with a resistivity value of 50 - 250 Ωm. Limestone generally shows porosity with the possibility of caves or pores in the rock formation at Ek Leuntie Cave, making it vulnerable to damage from earthquakes, landslides, and subsidence. The results of the study are expected to provide updated information in efforts to develop the Ek Leuntie Cave geopark area.
MENINGKATKAN PERKEMBANGAN MOTORIK ANAK USIA 5-6 TAHUN MELALUI TARIAN TRADISONAL MINANG, MAOLAH TARI
Authors: Sari Diah Andika
Published: June 2024

Dari hasil observasi pada kelompok B, di TK Budi Mulia, Tangerang Selatan, masih banyak perkembangan motorik anak yang belum berkembang dengan baik. Banyak anak yang belum mempunyai keseimbangan gerak seperti: bergerak mengikuti  irama music; memindahkan tubuh ke segala arah, Berpindah dengan melangkah kedepan, dan kebelakang; melangkah ke samping kiri dan kanan; melangkah  silang ke depan dan ke belakang secara bergantian (langkah menyilang); berdiri diatas kedua lutut; melompat dengan sebelah kaki; melakukan gerakan menjinjit; koordinasi gerakan tubuh (mata-tangan dan kaki). Anak-anak di TK Budi Mulia mengenal tari Balet serta tarian modern lainnya, tapi belum mengenal tarian tradisional Indonesia. Untuk itu penelitian ini bertujuan untuk meningkatkan kemampuan motorik 12 orang anak usia 5-6 tahun di TK Budi Mulia, Tangerang Selatan, dengan memperkenalkan tarian tradisional Minang, Molah Manari. Gerakan pada Tarian Molah Manari sangat sesuai dengan perkembangan motorik anak usia 5-6 tahun. Penelitian ini menggunakan metode Penelitian Tindakan Kelas (PTK) Model Kemis Taggart. Kegiatan terdiri dari 3 siklus, dimana setiap siklus terdiri dari 3 kali pertemuan, dengan total pertemuan sebanyak 10 kali, selama 4 minggu. Dari hasil penelitian, setelah melakukan kegiatan menari Molah Manari, terjadi peningkatan kemampuan motorik anak. Awalnya pada saat obervasi di pra siklus, kemampuan anak rata-rata observarsi berada pada tahap 1.3 skala 4, atau 34%, berada pada tahap belum berkembang. Setelah Latihan menari tarian Molah Manari dengan 3 siklus, pada siklus 1 meningkat menjadi 1.9 skala 4, atau 48%, masih pada tahap mulai berkembang. Pada siklus 2 meningkat menjadi 2.5 skala 4, atau 62% yang berada pada tahap berkembang sesuai harapan. Pada siklus 3, meningkat menjadi 3.1 skala 4, atau 78% yang bearti berkembang sangat baik. Sehingga dapat terlihat peningkatan kemampuan motoric yang signifikan setelah melakukan kegiatan menari tradisional Minang, tarian Molah Manari.
Kualitas Tidur Berhubungan dengan Kejadian Anemia pada Siswa MTs Pondok Pesantren Terpadu Al-Fauzan Lumajang
Authors: Khoiroh Iswatin , Isnawati Iin Aini , Mariani Mariani , Yunita Rizka
Published: June 2024

The need for rest and sleep may only be fulfilled somewhat, as experienced by students in Islamic boarding schools. The tight study hours in Islamic boarding schools cause students to pay less attention to rest and sleep. Lack of fulfillment of rest and sleep needs is thought to have an impact on the emergence of physiological and psychological disorders, one of which is anemia. This study aims to explain the relationship between sleep quality and the incidence of anemia in MTs students at the Al-Fauzan Lumajang integrated Islamic boarding school. This research uses an analytical observational design with a cross-sectional approach. The sample in this study consisted of 121 respondents, whom the researchers selected using simple random sampling techniques. Data collection uses the Pittsburgh Quality Sleep Index (PSQI) to measure sleep quality and the Hb meter to measure the incidence of anemia. Data analysis used the Pearson chi-square test. The results of measuring the sleep quality of MTs students at the Al-Fauzan Lumajang Islamic boarding school showed that 92 students, or 76% of the total respondents, had poor sleep quality, and 29 students, or 24% of the total respondents, had good quality. The results of measuring the incidence of anemia showed that 99 students, or 81.8% of the total respondents, had anemia, and 22 people, or 18.2% of the total respondents, did not experience anemia. The results of data analysis show a significant relationship between sleep quality and the incidence of anemia in MTs students at the Al-Fauzan Lumajang integrated Islamic boarding school with ρ-value=0.000<0.05. The results of this study show the importance of paying attention to students' sleep quality in preventing and treating anemia. Sleep improvement measures can be implemented in Islamic boarding schools to improve students' sleep quality and reduce the incidence of anemia.
Penerapan Motivational Interviewing terhadap Peningkatan Stages of Change pada Pasien Penyalahgunaan NAPZA: Case Report
Authors: Damayanti Selvi Rizkia , Fahrizal Yanuar , Triyana Triyana
Published: June 2024

Subtance abuse abuse (Narcotics, Psychotropics, and other Addictive Substances) is carried out illegally and is a complex problem in Indonesia. Substance abuse can affect changes in a person's behavior which can be overcome with motivational interviewing (MI) interventions. This study aims to see the effect of the application  of motivational interviewing (MI) on the stages of change in behavior in patients who are being rehabilitated by drugs. This research method uses case reports to evaluate nursing implementation using motivational interviewing to improve behavior change in substance abuse patients who are being rehabilitated with three evaluations during 15 days of meetings. The stages of change  measurement tool used is the URICA questionnaire. The results of this study showed that the patient's URICA value on the first day was 11.2 (contemplation) which increased after being given a motivational interviewing  intervention on the fifteenth day to 12.9 (action). There is an influence between the application of motivational interviewing on increasing stages of change in subtance abuse patients so that it is hoped that this motivational interviewing can be used in a structured way.
Flood Mapping and Impact Assessment in Agricultural Land in Hoa Vang, Da Nang Using Remote Sensing and Google Earth Engine
Authors: Hanh Le Ngoc , Hoang Son Nguyen , Phuc Chi Lang Le , Van An Nguyen , Thi An Tran
Published: June 2024

: Hoa Vang district in Da Nang city, is frequently affected by floods. This research was conducted to generate a flood map in Hoa Vang District, Da Nang City, during the peak flooding period in 2022, using Sentinel 1 imagery on the Google Earth Engine (GEE) cloud computing platform. The research also aims to assess the impact of this flooding event on the productivity of agricultural land in the district. Based on the Sentinel 1 imagery's backscattering coefficient, the study utilizes the Otsu algorithm for thresholding to determine the flood inundated areas. The results indicated that the optimal Otsu threshold value range from -16.003 to -10.631 dB. In addition, to assess the reliability of the flood mapping results, the study utilizes the in-situ flood inventory data to calculate the overall error and Kappa index. The research findings indicate a high level of reliability with an overall accuracy of 0.89 and a Kappa index of 0.79. Based on this analysis, the study successfully established a flood map for the peak flooding period 2022 in the study area. Furthermore, the study assessed the impact of floods on agricultural land productivity in the communes of Hoa Vang District. The findings showed that the flooded agricultural land area was 1,979.8 hectares, accounting for 77.3% of the total flooded area and 2.7% of the total natural area, primarily concentrated in the southern communes of the district such as Hoa Tien, Hoa Chau, Hoa Phuoc, and Hoa Phong. These findings serve as scientific background for assessing further impacts of flooding on agricultural land in Hoa Vang District, Da Nang City.
NILAI-NILAI PENDIDIKAN KEAGAMAAN YANG TERKANDUNG DALAM PENGGUNAAN GELANG TRIDATU BAGI UMAT HINDU
Authors: Sanjaya I Komang Alit Adi
Published: June 2024

Agama Hindu merupakan agama yang paling banyak menggunakan simbol dalam ritualnya. Kehadiran simbol ini digunakan sebagai instrumen untuk menghubungkan diri dengan Tuhan untuk dapat meningkatkan sraddha dan bhakti. Simbol dalam Hindu berperan sebagai lambang keagamaan dan merupakan benda sakral karena melalui proses sakralisasi (pasupati). Salah satu simbol yang sering digunakan dalam pelaksanaan ritual agama Hindu adalah gelang tridatu. Gelang tridatu merupakan gelang sakral, yang terdiri atas tiga warna yaitu: merah, hitam, dan putih. Ketiga warna ini mewakili manifestasi Tuhan sebagai konsep Tri Murti. Nilai-nilai yang terkandung dalam penggunaan gelang tridatu oleh umat Hindu diantaranya nilai religius, nilai komunikasi, nilai estetika, nilai ketenangan dan kenyamanan, dan nilai militansi. Penggunaan gelang tridatu dimaknai sebagai media realisasi diri yang dapat menumbuhkan kesadaran akan jati dirinya sebagai umat Hindu. Gelang tridatu adalah representasi dari Tri Murti, Tri Kona, Tri Pramana, dan Tri Kaya Parisudha yang dapat membangkitkan kesadaran umat. Penggunaan gelang tridatu juga berperan sebagai media komunikasi kultural. Penggunaan benang tridatu dalam kehidupan sehari-hari secara tidak langsung dapat mengkonstruksi sebuah identitas. Identitas yang menyatakan jika penggunanya adalah seorang umat Hindu. Dalam gelang tridatu juga terkandung nilai estetika karena keindahan kombinasi warna yang dihasilkan sehingga menghasilkan aura atau vibrasi positif dari simbol kekuatan Tri Murti. Umat yang menggunakan gelang tridatu akan merasa lebih aman dan tenang serta merasa terlindungi dari hal-hal yang bersifat negatif terutama secara niskala. Umat Hindu dapat menunjukkan rasa militansinya dengan menghadirkan  penanda dalam keutuhan agama dan juga sebagai pembeda dengan umat agama lainnya melalui menggunakan gelang tridatu
EKSISTENSI PANYAWANGAN PURA KAHYANGAN JAGAT TUNGGUL BESI DALAM KEHIDUPAN SOSIAL MASYARAKAT HINDU DI DESA ADAT ASAHDUREN JEMBRANA BALI
Authors: Sudharka Utama I Komang
Published: June 2024

Hubungan harmonis antara manusia dengan Ida Sang Hyang Widhi Wasa (Parhyangan) diaktualisasikan dengan mendirikan bangunan suci yang disebut Pura. Salah satu pura Kahyangan Jagat yang belum banyak dikenal masyarakat Hindu adalah Pura Kahyangan Jagat Tunggul Besi yang terletak di Desa Adat Temukus Karangasem. Pada Tahun 1933, sebanyak 10 KK warga pengempon yang pindah ke Desa Adat Asahduren Jembrana mendirikan Pura Panyawangan Kahyangan Jagat Tunggul Besi. Semula kegiatan sosial keagamaan di Pura Panyawangan ini berjalan harmonis. Namun akhir-akhir ini timbul konflik sosial atas eksistensi Pura Panyawangan ini. 
Rumusan masalah dalam penelitian ini ada 3 yaitu bagaimana sejarah berdirinya Panyawangan ini, bagaimana eksistensi Pura Panyawangan ini berkembang menjadi konflik sosial, dan bagaimana upaya penyelesaian konflik sosial yang terjadi. Teori-teori yang digunakan untuk membedah permasalahan adalah Teori eksistensialisme, Teori Religi dan Teori Konflik. Karya ilmiah ini merupakan penelitian kualitatif, penentuan informan dengan metode purposive, dan dikumpulkan dengan teknik observasi partisipatif, studi dokumen, dan studi kepustakaan. Data yang terkumpul, dianalisis dengan teknik deskriptif kualitatif.
Adapun hasil yang diperoleh dari penelitian ini adalah : (1) Pura penyawagan ini dibangun pada tahun 1933 oleh 10 KK yang disebabkan pada masa itu sangat sulit mendapatkan transportasi untuk kegiatan sosial keagamaan di pura asal; (2) Eksistensi Pura Panyawangan ini yang semula harmonis kemudian timbul konflik karena adanya keinginan beberapa warga pengempon untuk melakukan pralina dan mengembalikan fungsi pura ini ke Pura asal di Karangasem; (3) Upaya penyelesaian konflik sosial ini adalah dengan melibatkan pihak yang bisa sebagai katup penyelamat untuk melakukan dialog-dialog sehingga didapatkan kesepakatan baik untuk jangka pendek maupun jangka panjang, sehingga kehidupan sosial keagamaan para pengempon bisa harmonis sesuai ajaran Tri Hita Karana. 
Kata kunci : Tri Hita Karana, Eksistensi, Konflik
PŪJĀ TRISANDHYĀ DI DESA ADAT TONJA KECAMATAN DENPASAR UTARA KOTA DENPASAR
Authors: Tuty Setiarsih Dewa Ayu Putu , I Ketut Donder  , I Gusti Made Widya Sena
Published: June 2024

Konsep ibadah merupakan aspek yang sangat penting bagi setiap umat beragama. Cara sembahyang umat Hindu di Indonesia dibimbing dengan melakukan Pūjā Trisandhyā agar mengetahui dan merasakan atau mengenali sifat-sifat dan kemahakuasaan Tuhan sesuai dengan keyakinan dan kepercayaannya. Pūjā Trisandhyā tergolong dalam upasana dan merupakan jalan bhakti yoga. Mantra Pūjā Trisandhyā hanya dikumandangkan melalui alat pengeras suara, tanpa adanya usaha untuk memberikan edukasi terkait pemahaman yang mendalam di dalamnya. Tujuan penelitian ini untuk memperkaya ulasan Trisandhyā dan mengeksflorasi fenomena berkaitan dengan implementasi Pūjā. Metode yang digunakan dalam penelitian ini adalah metode kualitatif dengan pendekatan filosofis dan fenomenologi. Hasil dari penelitian ini menunjukkan implementasi Pūjā Trisandhyā di Desa Adat Tonja cenderung dipandang sebagai hal yang biasa yang wajib dipraktikkan untuk melengkapi persembahyangan dalam sebuah ritual piodalan. Jika tidak dilaksanakan tiga kali sesuai dengan ketentuan waktu, sebagian masyarakat merasa hal tersebut tidak/bukan menjadi masalah, implementasi puja sudah tergantikan dengan aktivitas yang lain; seperti mebanten canang, sembahyang dalam hati, ngayah di banjar untuk kegiatan suka dan duka, bekerja mencari nafkah dan sebagainya.
 Kata Kunci: Pūjā Trisandhyā, Desa Adat Tonja
RELASIONALITAS ANAK RONA-ANAK WINA DALAM UNGKAPAN TOE SALANG TUAK-WAE TEKU TEDENG: TINJAUAN FILSAFAT ARMADA RIYANTO
Authors: Hamat Yulianus Evantus , Riyanto F. X. Armada
Published: June 2024

Fokus utama tulisan ini ialah menggali makna filosofis atas Go’et toe salang tuak-wae teku tedeng dalam relasi Anak rona-anak wina. Proses penggalian dan pendalaman ini dilakukan dalam terang filsafat relasionalitas Armada Riyanto. Konsep filosofis Armada Riyanto menjadi pisau bedah yang dipakai penulis untuk memahami go’et toe salang tuak-wae teku tedeng dalam bingkai relasionalitas. Upaya pendalaman ini didasarkan pada fenomena pergeseran makna belis yang berdampak pada problem relasi keduanya. Atas dasar fenomena ini, penulis menawarkan konsep filosofis go’et toe salang tuak-wae teku tedeng sebagai upaya untuk menjernihkan kembali cara pandang terhadap makna belis di kalangan orang Manggarai. Metode yang digunakan adalah pendekatan studi pustaka disertai dengan pembacaan kritis atas karya dari Armada Riyanto. Tulisan ini menemukan bahwa relasi antara anak rona-anak wina akan terjalin dengan harmonis tatkala pemahaman akan belis tidak direduksi pada upaya pemiskinan keluarga anak wina tetapi lebih kepada penghormatan kepada wanita serta sarana untuk merekatkan hubungan antara anak rona dengan anak wina dalam suatu perkawinan.
PERIKOP ANAK YANG HILANG (LUK 15:11-24) DALAM PERSPEKTIF HIERARKI NILAI MAX SCHELER DAN RELEVANSI PADA ZAMAN SEKARANG
Authors: Babtista Thomas Rosario
Published: June 2024

Di zaman yang serba instan ini, banyak orang-orang yang mempertimbangkan sebuah keputusan dengan suatu nilai kenikmatan. Jelas penilaian seperti ini adalah nilai yang sangat dangkal dan artifisial. Seharusnya penilaian didasari pada nilai-nilai yang luhur.  Dalam teori hierarki nilainya, Max Scheler mengemukakan ada empat hierarki nilai, yaitu nilai kenikmatan, nilai kehidupan atau vitalitas, nilai spiritual, dan nilai kekudusan. Hierarki yang paling rendah adalah nilai kenikmatan dan yang paling tinggi adalah nilai kekudusan. Nilai ini pasti ada dalam kehidupan, karena ini adalah suatu makna dibalik tindakan. Hal ini tampak dalam perumpamaan “Anak Yang Hilang” (Luk 15:11-24) yang diceritakan oleh Yesus bagi para pemungut cukai dan orang berdosa. Ada suatu hierarki nilai yang ditampakkan dalam penokohan dan perjalanan batin dari tokoh-tokoh teks tersebut. Dengan mendalami teks Luk 15:11-24 dan disandingkan dengan teori hierarki nilai Max Scheler, penulis berharap dapat membantu setiap orang pada zaman instan ini untuk lebih mempertimbangkan berbagai macam hal dari nilai-nilai yang paling luhur. Ada dua metode yang dilakukan dalam penulisan teks ini, yaitu studi Pustaka dan hermeneutika. Metode ini diharapkan bisa membantu penulis dalam menganalisis dan menafsirkan teks secara terperinci dan holistik. Secara garis besar, penulis menemukan bahwa tokoh Anak Bungsu mengalami peningkatan dalam penghayatan nilai-nilai, yaitu berawal dari nilai kenikmatan, hingga nilai spiritual. Teks ini ditutup dengan penghayatan nilai kekudusan yang dilakukan oleh Bapa. Dengan kata lain, teks ini memberikan suatu penghayatan nilai yang utuh dari hierarki nilai Max Scheler.
WHEN PHILOSOPHERS DISCUSS HISTORY: THE THOUGHTS OF HERMENEUTIC PHILOSOPHERS IN EXAMINING HISTORY
Authors: Mahardika Moch. Dimas Galuh
Published: June 2024

The analysis of hermeneutics has been a subject of great interest and research among scholars, who have documented their findings in numerous books and scientific journals. In this article, the author endeavors to provide a deeper insight into hermeneutics as a tool and approach for examining the past. The ideas of prominent philosophers from the 19th and 20th centuries have contributed significantly to advancing historical research, particularly in comprehending past human activities and behaviors. Several renowned philosophers, including Dilthey, Gadamer, and Collingwood, have provided invaluable insights into interpreting and explaining history based on existing textual sources. This literature-based study seeks to explore the perspectives and discussions of these philosophers on the hermeneutic approach to interpreting history.
Relative leaf expansion rate as an indicator of compensatory growth of defoliated vines of Prokupac (Vitis vinifera L.)
Authors:   Academy of Vocational Studies Southern Serbia, Department of Agricultural and Food Studies in Prokuplje, Serbia, CIRKOVIC DUSICA , MATIJASEVIC SASA ,   University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia, CIRKOVIC BRATISLAV ,   University of Pristina – Kosovska Mitrovica, Faculty of Agriculture, Kopaonicka bb, 38219 Lesak, Serbia, BESLIC ZORAN ,   University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Published: June 2024

Reaction of the grapevine to the early defoliation is to mitigate its effects through compensatory growth by producing more lateral shoots with a greater number of leaves. In this study, we evaluated the usage of non-destructive and continuous measurements of mean and lateral leaf area on the same shoots for the purpose of monitoring the leaf area development and calculating relative leaf expansion rate - RLER during vegetation. This study has shown that the grapevine's ability to recover its leaf area after defoliation depends mainly on the time when the defoliation occurs. Early defoliated vines had time to compensate removed leaves by producing more lateral shoots with a greater number of leaves, which resulted in a larger leaf area. With the decrease in the intensity of shoot growth during vegetation, the recovery ability decreases, therefore, compensatory growth is not enough to restore the reduced leaf area. Based on the value of RLER, its defoliation is performed in the period of intensive growth of shoots, it affects the stagnation of the emergence of new shoots and leaves over several days, followed by a period of re-growth. Very slow or no growth of shoots and leaves occurred after the veraison stage.
Use of Stabilized rice bran for preparing Beef Burger
Authors:   Rice Research Section, Field Crops Research Institute, Agric. Res. Center, Giza, Egypt, EL-DAKARANY HAGER IBRAHIM , EL-ABD ABDEL MOATY ,   Rice Research Section, Field Crops Research Institute, Agric. Res. Center, Giza, EL-BANA MOHAMED AHMED ,   Food Technology Research Institute, Agricultural Research Center, Giza, 12611, Egypt, SALAMA MOHAMED ABDELBASET , ABDELHADY SAHAR RAMADAN ,   Food Technology Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33511, Egypt, GHAZI ADEL ,   Food Technology Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33511, Egypt
Published: June 2024

This study was performed to investigate the effect of thermal processing (microwave oven, roasting by dry heat at 130°, for 10 and 20 min. and steaming by using an autoclave under atmospheric pressure for 10 and 20 min.) on the stability of rice bran and the utilization of microwave stabilized rice bran as fat replacers in preparing a low-fat beef burger. The obtained results revealed that: rice bran contained high levels of carbohydrates and moderate amounts of ether extract and crude protein. No differences were detected in the refractive index of rice bran oil among the stabilization procedures, whereas slight differences were found in the case of specific gravity. When rice bran oil was subjected to stabilized procedures, the acid value, iodine value, peroxide value, saponification value, and unsaponifiable matter were reduced. Moreover, the total saturated fatty acids content of rice bran oil increased as a result of subjecting to stabilization procedures whereas the total unsaturated fatty acid content decreased. The results show that stabilized rice bran is effective in improving the chemical and functional characteristics of a beef burger.
PETase from Ideonella sakaiensis: towards facile bacterial expression system
Authors:   Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engi¬neering, Babes-Bolyai University, Arany Janos Street 11, RO-400028 Cluj-Napoca, Romania, BOROS KRISZTINA , HORVATH ILKA ,   Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engi¬neering, Babes-Bolyai University, Arany Janos Street 11, RO-400028 Cluj-Napoca, Romania, ROTARU CATALINA ,   Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engi¬neering, Babes-Bolyai University, Arany Janos Street 11, RO-400028 Cluj-Napoca, Romania, TOMOIAGA RALUCA BIANCA ,   Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engi¬neering, Babes-Bolyai University, Arany Janos Street 11, RO-400028 Cluj-Napoca, Romania, TOȘA MONICA IOANA ,   Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engi¬neering, Babes-Bolyai University, Arany Janos Street 11, RO-400028 Cluj-Napoca, Romania, BENCZE LASZLO-CSABA ,   Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engi¬neering, Babes-Bolyai University, Arany Janos Street 11, RO-400028 Cluj-Napoca, Romania
Published: June 2024

One of the most promising PET-hydrolysing enzyme, IsPETase, originates from 'plastic-consuming' bacteria Ideonella sakaiensis and operates at moderate temperatures (30-40 °C) in aqueous environment, showing far higher hydrolysing efficiency than several cutinases. Accordingly, the development of proper recombinant expression system providing facile access to the isolated/purified IsPETase is also of high interest. In our aim to produce active recombinant IsPETase, the unsuccessful expression registered by employing several reported expression systems, directed us towards the optimization/ development of a facile/accessible recombinant expression system suitable for IsPETase production. Testing several plasmid constructs, providing different N- or C-terminal affinity tags for the expression of full-length or truncated (residues 28-290) IsPETase and various E. coli expression hosts, revealed several non-reported issues hindering the recombinant expression of IsPETase. After optimization of the construct and expression host, the use of N-terminal His-tag and Rosetta-gami B expression host provided recombinant IsPETase in high purity and titer-yield. The thermal denaturation profile and PET-hydrolysing activity of the obtained IsPETase agree with the reported data, supporting proper folding of the purified protein. The results support that the in vivo folding process of IsPETase might be differently affected among the different E. coli host strains, moreover, underline the importance of the proper selection of the cloning strategy for the successful expression of the IsPETase.
In vitro elicitation supports the enrichment of 2H4MB production in callus suspension cultures of D. hamiltonii Wight & Arn
Authors:   Plant Cell Biotechnology Department, Council of Scientific and Industrial Research — Central Food Technological Research Institute, Mysore — 570 020, Karnataka, India, KOPPADA UMASHANKAR , MATAM PRADEEP ,   Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India, PARVATAM GIRIDHAR ,   Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India
Published: June 2024

Influence of elicitation on in vitro production of 2-Hydroxy-4-Methoxy Benzaldehyde (2H4MB), a structural isomer of vanillin from callus suspension cultures of Decalepis hamiltonii was investigated. In vitro culture conditions were optimized to induce callus, suspension culture and biomass followed by metabolite production. Suspension cultures were established using leaf generated friable callus. Maximum content of 2H4MB production 0.079 ± 0.01 mg 100g-1 DW and biomass 197.5 ± 1.5 g1_,-1 were observed by 4th week of culturing. Elicitation was induced to suspension cultures by using m¬topolin (mT), sodium nitroprusside (SNP), and pectin individually at different concentrations. m¬topolin (15 itiM), pectin (15 itiM) and SNP (10 itiM) supported 0.31 mg100g-1DW, 0.27 mg100g-1DW and 0.21 mg100g-lof 2H4MB production respectively by 4th week. This data infers that the elicitation improves 2H4MB content in callus suspension cultures of D. hamiltonii.
Effects of prebiotics on acid and bile resistance of Bifidobacterium lactis and Lactobacillus acidophilus probiotic bacteria
Authors:   Olive Research Institute, Izmir, Turkey, MUMCU AYLA , TEMIZ AYHAN ,   Hacettepe University, Faculty of Engineering, Department of Food Engineering, Ankara, Turkey
Published: June 2024

The effects of prebiotics on acid and bile resistance of probiotics were evaluated. Two commercial strains, Bifidobacterium lactis BB-12 and Lactobacillus acidophilus LA-5 had higher acid and bile resistance characteristics than the ATCC strains. B. lactis BB-12 was the most resistant to acid and bile and showed higher survival capacity. The counts of B. lactis BB-12 viable cells in pH 2 medium decreased by 2.7 log unit while the viability of other strains decreased by 4.5-7 log units. The counts of B. lactis BB-12 viable cells in the bile medium after 6 and 24 hours of incubation were determined as 6.1 and 2.8 log CFU/ml, respectively, while no viable bacteria could be determined for the other strains after 24 hours of incubation. The acid and bile resistance of probiotic bacteria remarkably varied with the type of prebiotics. Acid and bile resistance properties of L. acidophilus LA-5 and L. acidophilus ATCC 4356 were found higher when INU was present in the growth medium. Acid and bile resistance properties of B. bifidum ATCC 15969 was higher when the medium contained GOS whereas acid and bile resistance of B. lactis BB-12 was found to be higher when medium contained XOS.
Representações do prazer e do corpo feminino em Marina Colasanti e Fabiana Faleiros
Authors: Marzani Caroline , Caetano de Almeida Rogério
Published: June 2024

Este artigo pretende revelar imagens do corpo feminino e as questões ligadas ao prazer da mulher em poemas de Marina Colasanti e Fabiana Faleiros. Para tanto, utilizamos as obras Fino sangue (2005), de Marina Colasanti, e O pulso que cai e as tecnologias do toque (2016), de Fabiana Faleiros. Ambas as poetas trazem o olhar sensível e erótico (BATAILLE, 2017) sobre a autodescoberta corporal feminina. Analisando a história da sexualidade (FOUCAULT, 2009; 2015) e as questões de gênero, corpo (BUTLER, 2016; HARAWAY, 2009) e lugar de fala (DOLAR, 2015), discutimos a desconstrução de tabus sobre o corpo feminino.
Guido Oldani’s poetics of terminal realism
Authors: Chiara Canta Carmelina , Oldani Guido
Published: June 2024

Terminal Realism (TR) is an avant-garde movement founded in the literary-artistic field in 2010 by Guido Oldani, a contemporary Italian poet who aims to critically represent the globalised civilisation of the third millennium. In particular, TR presents itself as a movement of civil commitment. The Founding Manifesto is presented in 2010 and sets out the principles of poetics, the relationship of man with the object, the artificial versus the natural, the piling up of people and things, and pandemics. The poetry of the new millennium therefore expresses the artificial and the artefact using a new poetic language including: the stacking, the Inverted simile and the irony.
Por que estudar as antologias?
Authors: Junqueira Guimarães Raquel Beatriz , Hélder Pinheiro Alves José
Published: June 2024

Apresentação à Texto Poético 42.
KERAGAAN MORFO-FISIOLOGI SERTA KOMPONEN HASIL DAN HASIL GENOTIPE PADI PADA METODE BUDIDAYA TANAM BENIH LANGSUNG LAHAN BASAH
Authors: Rifqi Fauzi Ahmad , Junaedi Ahmad , Lubis Iskandar , Ghulamhdi Munif , Aswidinnoor Hajrial
Published: June 2024

Sistem budidaya tanam benih langsung (Tabela) telah banyak dipraktikkan di berbagai negara Asia karena dianggap lebih hemat air dan tenaga kerja serta memberikan keuntungan yang lebih baik dibandingkan sistem pindah tanam. Namun demikian, perbaikan pengelolaan praktik sistem budidaya ini masih diperlukan khususnya dalam pola tanam dan penggunaan varietas yang sesuai. Penelitian ini bertujuan untuk mengkaji karakter fisiologi, komponen hasil dan hasil genotipe padi pada sistem budidaya pindah tanam dan Tabela. Penelitian ini dilaksanakan di lahan dengan sistem budidaya pindah tanam dan Tabela (sebar alur dan sebar permukaan) serta menggunakan 4 galur unggulan dan satu varietas nasional. Sebanyak 15 kombinasi perlakuan dirancang dengan rancangan kelompok acak lengkap (RAKL) dengan 3 ulangan setiap perlakuan. Hasil penelitian menunjukkan bahwa padi yang ditanam dengan Tabela laju fotosintesis yang sama dengan tanaman padi pada budidaya pindah tanam, namun menghasilkan panjang malai, jumlah gabah per malai, dan jumlah gabah isi yang lebih rendah serta waktu berbunga lebih cepat. Genotipe IPB193-F-17-2-3 menghasilkan produktivitas tertinggi pada budidaya tanam benih langsung lahan basah (metode alur dan sebar) dan lahan kering (metode alur). Genotipe IPB193-F-38-2-1 menghasilkan produktivitas tertinggi pada sistem pindah tanam dan tanam benih langsung lahan kering (metode sebar). Produktivitas varietas Ciherang pada sistem tanam benih langsung (metode alur) tidak berbeda dengan sistem pindah tanam. Temuan ini mengindikasikan bahwa sistem tanam benih langsung dengan metode alur cukup menjanjikan untuk dikembangkan lebih lanjut.
Features of tall middle maturing soybean cultivar Jaguar with a trait of accelerated growth of biomass
Authors:   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Lukomets V.M. , Zelentsov S.V. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Moshnenko E.V. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Trunova M.V. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Budnikov E.N. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Saenko G.M. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Dul’tsev E.P. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Ramazanova S.A. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops
Published: June 2024

The tall, middle maturing soybean cultivar Jaguar with a trait of accelerated formation of aboveground biomass was obtained as a result of individual selection in F4 from the hybrid combination Vilana × D-7/10 by tall growth (> 150 cm) of the stem, large seeds, middle maturity and lodging resistance. Based on the results of variety testing in 2020–2023, the soybean cultivar Jaguar exceeded the standard cultivar Vilana by 0.26 t/ha of yield. With an average vegetative period of 123 days at the latitude of Krasnodar (45°), the height of plants of this cultivar is 138 cm, varying over the years from 120 to 154 cm. In optimal moisture conditions, the weight of 1000 seeds is 160–190 g. The phenomenon of accelerated formation of aboveground biomass of plants of the cultivar Jaguar y in optimal cultivation conditions is confirmed by a 44% more intense daily increase in plant height and a 55% more intense daily increase in aboveground biomass compared to these indicators for the standard cultivar Vilana. The new tall, middle maturing soybean variety Jaguar, with a trait of accelerated formation of aboveground biomass, is characterized by increased responsiveness to additional moisture by increasing the plant height to ultra-high, increasing the number of nodes, beans and seeds, seed size and weight, and yield. It has increased resistance of tall plants to tilting and lodging due to excessive moisture due to the increased mechanical strength of the stem. Due to its tall growth and high positive response to additional moisture supply, it is suitable for cultivation in the southern foothill and central zones of the Krasnodar region, with irrigation throughout the North Caucasus and Lower Volga regions of the Russian Federation, as well as in the republics of Central Asia and Transcaucasia.
Increase of breeding efficiency of soybeans with reduced response on a day length taking the example of a cultivar Lipchanka
Authors:   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Zelentsov S.V. , Paspekov D.I. ,   Lipetsk Research Institute of Rapeseed, Moshnenko E.V. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Trunova M.V. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Tevchenkov A.A. ,   Lipetsk Research Institute of Rapeseed, Budnikov E.N. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Saenko G.M. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Ramazanova S.A. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Kuzenkova V.V. ,   Lipetsk Research Institute of Rapeseed
Published: June 2024

A very early maturing soybean cultivar Lipchanka with a reduced response on a day length was isolated from the hybrid combination F4 D-12/14 × L-247 by traits of productivity, cold resistance, photoperiodic sensitivity, and the morphotype of the flower ovary style. The Lipchanka variety belongs to a very early maturity group, at the latitude of Krasnodar (45°) it ripens in 95 days, and in terms of yield exceeds the standard cultivar Barguzin by an average of 0.11 t/ha. In a competitive variety testing in Krasnodar in 2021–2023 its yield was 1.32–3.19 t/ha. In a competitive variety trial in Lipetsk at a latitude of 52°, the duration of the growing season of this cultivar averaged 105 days with a yield of 2.41–2.67 t/ha, which exceeded the standard variety Barguzin by 0.09 t/ha. Under the conditions of the Kaluga region, at a latitude of 54°, the cultivar Lipchanka had a growing season of 113 days and successfully ripened until mid-September. The height of Lipchanka plants at the latitude of Krasnodar was 70–85 cm. In the Lipetsk region, the plant height of Lipchanka varied within 70–95 cm. In the conditions of the Kaluga region, the height of the cultivar Lipchanka was 71 cm. The new very early maturing soybean cultivar Lipchanka has reduced sensitivity to the range of day lengths formed at latitudes of 45–54°, high yield in the long-day conditions of the Central Black Earth region, and successful ripening in the conditions of the Central region of the Russian Federation. Such features of the cultivar allows to recommend it for cultivation in the Central, Volga-Vyatka, Central Black Earth, North Caucasus, Middle Volga, Lower Volga, Ural, and West Siberian regions of the Russian Federation.
Ecological stability and plasticity of spring brown mustard variety of VNIIMK breeding
Authors:   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Trubina V.S. , Serdyuk О.А. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops, Gorlova L.А. ,   V.S. Pustovoit All-Russian Research Institute of Oil Crops
Published: June 2024

Analysis of ecological plasticity and stability must be a base in the development of new genotypes of spring brown mustard. The purpose of the research was a comparative characteristic of spring brown mustard cultivars bred at the V.S. Pustovoit All-Russian Research Institute of Oil Crops by calculated statistic parameters of adaptability, ecological plasticity, stability, stress-resistance, and genetic flexibility in the central zone of the Krasnodar region in 2018–2022. We studied commercial (Nika, Yunona, Gorlinka, Galateya) and promising (VN11/18, VN-27/18, VN-9/18, and VN-25/18) cultivars. The indicators of ecological plasticity and stability were calculated basing on seed yield of spring brown mustard cultivars for five years by a methodology of Eberhart S.A., Rassell W.A. as reworded by Zykin V.А. To calculate a coefficient of a linear regression, we determined environment indexes, which characterized variability of conditions of the cultivar cultivation. The stress-resistance and genetic flexibility were determined by an equation of Rossille A.A., Hamblin J. as presented by Goncharenko А.А. The potential productivity and adaptability were revealed by a methodology of Zhivotkov L.А. The weather conditions in 2020 and 2021 were the most favorable, environmental index had the highest positive meaning and was equal to 1.74 and 1.82 under the highest average yield of brown mustard, which was equal to 1.92 and 1.93 t/ha, respectively. The weather conditions of 2018 and 2022 were less favorable for estimation of brown mustard samples (Ij = -1.71 and -2.00). A comparative analysis of brown mustard cultivars bred at the V.S. Pustovoit All-Russian Research Institute of Oil Crops by parameters of ecological plasticity and stability in the Krasnodar region showed the cultivars Yunona, VN-27/18, and VN-11/18 had the highest plasticity, and Yunona and Galateya demonstrated the highest stability and adaptability to declining of cultivation conditions. Thus, the cultivars Yunona and Galateya were characterized with high yield, plasticity, stability, genetic flexibility, and adaptability. In contrast conditions, the promising cultivar VN-9/18 had high indicators of resistance to stress factors, yield, and adaptability coefficient.
Ocular filariasis in the form of a conjunctival granuloma – A rare case report from non-endemic part of India
Authors: Sarkar Annesha , Das Dipankar , Islam Saidul , Deka Apurba
Published: June 2024

Filariasis, a predominantly lymphatic disease can rarely have ocular involvement in endemic as well as a few non-endemic zones of the Indian subcontinent. It has also been reported from countries of South East Asia. In India, Wuchereria bancrofti and Brugia malayi are the nematodes causing filariais. Ocular involvement can happen in a wide spectrum, involving lacrimal glands, conjunctiva and fornices, cornea, anterior chamber, and vitreous cavity. We report a case of adult filarial worm (B. malayi) inside a conjunctival granuloma in a lady hailing from a rural, non-endemic part of the country. The lady presented with a short history of ocular irritation and redness. Clinical examination revealed a small granuloma-like lesion with surrounding inflammation over the conjunctiva. The rest of the anterior segment as well as the posterior segment was fairly within normal limits. The history as well as clinical examination was not supportive of a parasitic infestation, leading the doctors to treat the lesion as an allergic disease. However, the lesion remained unresolved, and surgical excision was undertaken. On surgical intervention, a live worm was spotted inside the lesion. Parasitological evaluation of the worm was confirmatory of adult filaria (B. malayi). The patient responded to a combination therapy of oral diethylcarbamazine and albendazole.
Clinical profile and risk factors for type-2 diabetes – A cross-sectional study
Authors: Okoro Peace N. , Olarinoye Kola , Esan Arinola
Published: June 2024

Objectives

Type 2 diabetes is a chronic metabolic disorder characterised by elevated blood glucose levels due to insulin resistance or insufficient insulin production. Understanding the prevalence, characteristics, and markers of this disease is essential for effective prevention, management and treatment. The study aims to explore the association between type 2 diabetes and its prevalence, characteristics and markers.

Material and Methods

Adults with type 2 diabetes and matched healthy controls were enrolled in the study. Statistical calculations were used to establish the sample size. Information on demographics, physical examinations and lab tests were collected. We assessed plasma glucose, glycated haemoglobin (HbA1c), serum lipids and serum DPP4.

Results

The mean age (SD) of the population under research was 55.7 (6.12) years for the study subjects and 55.7 (6.11) years for the controls. A positive family history of diabetes was present in 34 (or 33%) of the diabetes patients compared to 11 (or 11%) of the non-diabetic patients (p 0.001). The mean HbA1c in the diabetic group was substantially greater than that of the non-diabetic controls (5.47 1.89%), as expected (7.23 2.69%), p 0.001. It’s interesting to note that total cholesterol was markedly higher in the diabetes participants (5.59 2.24 mmol/L) than in the non-diabetic controls (6.48 1.54 mmol/L), p = 0.001.

Conclusion

Due to common risk factors and underlying mechanisms, type 2 diabetes and hypertension may be related, as suggested by the high prevalence of hypertension in the diabetic group. Type 2 diabetes was substantially related to elevated HbA1c and fasting plasma glucose levels, indicating poor glycaemic control. The higher mean serum Dipeptidyl peptidase-4 (DPP4) level in the group with type 2 diabetes shows a link between high DPP4 levels and the disease, which may have an effect on incretin hormone activity, insulin resistance and therapeutic options.
THE EFFECT OF FEATURE EXTRACTION ON COVID-19 CLASSIFICATION
Authors: Hamaamin Rebin A. , Wady Shakhawan H. , Kareem Sangawi Ali W.
Published: June 2024

X-ray imaging stands as a prominent technique for diagnosing COVID-19, and it also serves as a crucial tool in the medical field for the analysis of various diseases. Numerous approaches are available to facilitate this analysis. Among these techniques, one involves the utilization of a Feature Extractor, which effectively captures pertinent characteristics from X-ray images. In a recent study, a comprehensive examination was conducted using 25 distinct feature extractors on X-ray images specific to COVID-19 cases. These images were categorized into two classes: COVID-19-positive and non-COVID-19. To enable a thorough evaluation, a sequence of machine learning classifiers was employed on these categorized images. The outcomes derived from this experimentation gauged the magnitude of impact that each individual feature exerted on COVID-19-related imagery. This assessment aimed to determine the efficacy levels of various feature extractors in terms of detection capability. Consequently, a distinction emerged between the more effective and less effective feature extractors, shedding light on their varying degrees of contribution to the detection process. Moreover, the comparative performance of different classifiers became evident, revealing the classifiers that exhibited superior performance when measured against their counterparts.
Evaluating the Linear Viscoelasticity of Vitrimers Using Start-up and Cessation Tests
Authors: Cho Noah , El-Zaatari Bassil , Gokarn Ria , Van Zee Nicholas , Chapman Steven , Craig Stephen , Kalow Julia , Richards Jeffrey
Published: June 2024

Stress relaxation experiments are often performed on reprocessable polymer networks to evaluate their characteristic relaxation time, which represents the bond lifetime in the absence of structural contributions. While traditional cessation tests employ the application of an instantaneous small strain, the strain amplitude and the rate of strain applied during startup are known to influence the subsequent stress relaxation behavior. Here we illustrate this phenomenon using vitrimers with characteristic bond exchange times spanning seconds to hours. Our experiments show that controlling the dimensionless strain amplitude and shear rate applied during startup is critical to obtaining the true distribution of relaxation times for non-Maxwellian materials. In particular, consistent with rheology performed on thermoplastics, we find that experiments performed at small Deborah numbers, the dimensionless start-up timescale, and small Weissenberg numbers, the dimensionless accumulated strain, result in a stress relaxation time consistent with that observed in SAOS tests.
Digital Skills in Chemical Education
Authors: McCluskey Andrew , Rivera Miguel , Mey Antonia
Published: June 2024

The role of computing in the chemical sciences is changing. Previously the domain of the theoretical or computational chemist, advanced digital skills, including data analysis and simulation, are becoming extremely relevant to all. Here, we discuss the importance of integrating computing and digital skills into an undergraduate chemistry program and highlight some work currently being carried out to achieve this.