bluuebunny/crossref_metadata_2025 huggingface dataset:
The optimization of gold nanoparticles–horseradish peroxidase as peroxidase mimic using central composite design for the detection of hydrogen peroxide
Authors: Talapphet Natchanok , Huh Chang Soon
Published: February 2024

The oxidizing agent, hydrogen peroxide (H2O2), which is a part of reactive oxygen species (ROS) is well-known to contribute to oxidative stress-induced damage to biological molecules. An excess of free radicals can harm health and is associated with human diseases. Gold nanotechnology, a highly relevant nanomaterial, has been utilized as a new material in advanced sensor detection. In this study, colorimetric methods based on peroxidase enzymes were developed for measuring H2O2. The synthesized gold nanoparticles (AuNPs) showed a concentration of approximately 1.73 nM at a wavelength of 520 nm. The average diameter displayed a uniform size distribution, estimated at 18 nm, and an increase in the shell thickness of AuNPs-horseradish peroxidase (HRP) was observed in the TEM images. The AuNPs-HRP system demonstrated remarkable catalytic activity in the reaction of the chromogenic substrate tetramethylbenzidine (TMB) with H2O2, resulting in the production of an oxide product. The optimal conditions for the AuNPs-HRP system, as determined by central composite design (CCD), were a temperature of 25 °C and a pH of 7 within an 8 h period. A strong linear relationship was observed between different absorbance values and the H2O2 concentration, with a coefficient of determination of 0.9956. A portable platform was successfully used to determine H2O2 levels in beverages with recoveries ranging from 95.51% to 118.85%. These findings suggest that the AuNPs-HRP system could be applied to detect H2O2 in beverages.
ImmuneMirror: A machine learning-based integrative pipeline and web server for neoantigen prediction
Authors: Chuwdhury Gulam Sarwar , Guo Yunshan , Chiang Chi-Leung , Lam Ka-On , Kam Ngar-Woon , Liu Zhonghua , Dai Wei
Published: February 2024

Neoantigens are derived from somatic mutations in the tumors but are absent in normal tissues. Emerging evidence suggests that neoantigens can stimulate tumor-specific T-cell-mediated antitumor immune responses, and therefore are potential immunotherapeutic targets. We developed ImmuneMirror as a stand-alone open-source pipeline and a web server incorporating a balanced random forest model for neoantigen prediction and prioritization. The prediction model was trained and tested using known immunogenic neopeptides collected from 19 published studies. The area under the curve of our trained model was 0.87 based on the testing data. We applied ImmuneMirror to the whole-exome sequencing and RNA sequencing data obtained from gastrointestinal tract cancers including 805 tumors from colorectal cancer (CRC), esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma patients. We discovered a subgroup of microsatellite instability-high (MSI-H) CRC patients with a low neoantigen load but a high tumor mutation burden (> 10 mutations per Mbp). Although the efficacy of PD-1 blockade has been demonstrated in advanced MSI-H patients, almost half of such patients do not respond well. Our study identified a subset of MSI-H patients who may not benefit from this treatment with lower neoantigen load for major histocompatibility complex I (P < 0.0001) and II (P = 0.0008) molecules, respectively. Additionally, the neopeptide YMCNSSCMGV-TP53G245V, derived from a hotspot mutation restricted by HLA-A02, was identified as a potential actionable target in ESCC. This is so far the largest study to comprehensively evaluate neoantigen prediction models using experimentally validated neopeptides. Our results demonstrate the reliability and effectiveness of ImmuneMirror for neoantigen prediction.
Enhancing protein dynamics analysis with hydrophilic polyethylene glycol cross-linkers
Authors: Sun Min , Chen Jing , Zhao Chang , Zhang Lihua , Liu Maili , Zhang Yukui , Zhao Qun , Gong Zhou
Published: February 2024

Cross-linkers play a critical role in capturing protein dynamics in chemical cross-linking mass spectrometry techniques. Various types of cross-linkers with different backbone features are widely used in the study of proteins. However, it is still not clear how the cross-linkers’ backbone affect their own structure and their interactions with proteins. In this study, we systematically characterized and compared methylene backbone and polyethylene glycol (PEG) backbone cross-linkers in terms of capturing protein structure and dynamics. The results indicate the cross-linker with PEG backbone have a better ability to capture the inter-domain dynamics of calmodulin, adenylate kinase, maltodextrin binding protein and dual-specificity protein phosphatase. We further conducted quantum chemical calculations and all-atom molecular dynamics simulations to analyze thermodynamic and kinetic properties of PEG backbone and methylene backbone cross-linkers. Solution nuclear magnetic resonance was employed to validate the interaction interface between proteins and cross-linkers. Our findings suggest that the polarity distribution of PEG backbone enhances the accessibility of the cross-linker to the protein surface, facilitating the capture of sites located in dynamic regions. By comprehensively benchmarking with disuccinimidyl suberate (DSS)/bis-sulfosuccinimidyl-suberate(BS3), bis-succinimidyl-(PEG)2 revealed superior advantages in protein dynamic conformation analysis in vitro and in vivo, enabling the capture of a greater number of cross-linking sites and better modeling of protein dynamics. Furthermore, our study provides valuable guidance for the development and application of PEG backbone cross-linkers.
PREDAC-CNN: predicting antigenic clusters of seasonal influenza A viruses with convolutional neural network
Authors: Meng Jing , Liu Jingze , Song Wenkai , Li Honglei , Wang Jiangyuan , Zhang Le , Peng Yousong , Wu Aiping , Jiang Taijiao
Published: February 2024

Vaccination stands as the most effective and economical strategy for prevention and control of influenza. The primary target of neutralizing antibodies is the surface antigen hemagglutinin (HA). However, ongoing mutations in the HA sequence result in antigenic drift. The success of a vaccine is contingent on its antigenic congruence with circulating strains. Thus, predicting antigenic variants and deducing antigenic clusters of influenza viruses are pivotal for recommendation of vaccine strains. The antigenicity of influenza A viruses is determined by the interplay of amino acids in the HA1 sequence. In this study, we exploit the ability of convolutional neural networks (CNNs) to extract spatial feature representations in the convolutional layers, which can discern interactions between amino acid sites. We introduce PREDAC-CNN, a model designed to track antigenic evolution of seasonal influenza A viruses. Accessible at http://predac-cnn.cloudna.cn, PREDAC-CNN formulates a spatially oriented representation of the HA1 sequence, optimized for the convolutional framework. It effectively probes interactions among amino acid sites in the HA1 sequence. Also, PREDAC-CNN focuses exclusively on physicochemical attributes crucial for the antigenicity of influenza viruses, thereby eliminating unnecessary amino acid embeddings. Together, PREDAC-CNN is adept at capturing interactions of amino acid sites within the HA1 sequence and examining the collective impact of point mutations on antigenic variation. Through 5-fold cross-validation and retrospective testing, PREDAC-CNN has shown superior performance in predicting antigenic variants compared to its counterparts. Additionally, PREDAC-CNN has been instrumental in identifying predominant antigenic clusters for A/H3N2 (1968–2023) and A/H1N1 (1977–2023) viruses, significantly aiding in vaccine strain recommendation.
Prurigo nodularis: new insights into pathogenesis and novel therapeutics
Authors: Liao Viviane , Cornman Hannah L , Ma Emily , Kwatra Shawn G
Published: February 2024

Lay Summary

Prurigo nodularis (PN) is a chronic skin condition featuring extremely itchy nodules on the skin of the legs, arms and trunk of the body. PN affects approximately 72 per 100 000 people and the severe itch associated with the condition can negatively impact a person’s sleep, work and social life. However, the cause of PN remains unclear.

Current understanding of PN is based on imbalances in the immune system leading to widespread inflammation as well as dysregulation of the nerves in the skin. Immune molecules released from T cells [such as interleukin (IL)-4, -13, -31, -17, -22 and -31] increase systemic inflammation and are elevated in people with PN. Activated inflammatory cells (such as mast cells or eosinophils) may also release factors that promote inflammation, itch and neural changes within the skin. Neural dysregulation in PN features a lower density of itch-sensing nerve fibres in the epidermis (upper layer of the skin) and a higher density of itch-sensing nerve fibres in the dermis (lower layer of the skin). Because the pathogenesis of PN is not fully understood, the therapies available for PN have had limited success in reducing itch and nodules.

The only drug currently approved for PN in the USA and Europe is dupilumab, an IL-4Rα inhibitor that blocks signalling through IL-4 and IL-13, which is undergoing post-marketing surveillance. Other new drugs are being assessed in various phases of clinical trials, including nemolizumab, vixarelimab, barzolvolimab, ruxolitinib, abrocitinib, povorcitinib and nalbuphine.
Histamine- and pruritogen-induced itch is inhibited by a TRPM8 agonist: a randomized vehicle-controlled human trial
Authors: Lee Soyeon , Wei Edward T , Selescu Tudor , Babes Alexandru , Park Jinseo , Kim Jincheol , Chung Boyoung , Park Chunwook , Kim Hye One
Published: February 2024

Lay Summary

Managing itching and the effects of histamine can be difficult for people with allergies. Cooling the skin or applying menthol provides some relief from itch, but the way they work is not fully understood. Cooling agents interact with a protein called TRPM8 (also known as the ‘cold and menthol receptor’) and have shown potential for the management of itch. However, much of the research has been done on animals and has limitations when compared with human studies. Antihistamine medications can help with histamine-induced itching, but they may not work for other causes of itch.

This study investigated the effects of a specific TRPM8 agonist (a chemical that activates a receptor to produce a biologic response) gel called cryosim-1 on itch in human skin. To do this, we conducted tests on 30 healthy people using five different substances that cause itching. Participants rated the itch intensity and pain using a scale and we measured various aspects of their skin. The results showed that all substances caused significant itching compared to a control substance, but itchiness gradually decreased over time. Histamine and compound 48/80 also caused pain. However, when participants applied the TRPM8 activator gel before exposure, they experienced less itching and lower itch intensity versus the gel without the activator. There were no significant differences in pain between the TRPM8 activator and the gel without it.

In summary, our findings showed that activating TRPM8 receptors with a specific substance effectively relieved itching caused by various irritants on human skin. This suggests its potential as a treatment for itch-related conditions. Further research is needed to understand its mechanisms better and evaluate its effectiveness in real-life situations.
Mindfulness-Based Social Work and Self-Care with Social Work Professionals: Replication and Expansion of a Randomised Controlled Trial
Authors: Maddock Alan , McGuigan Karen , McCusker Pearse
Published: February 2024

Social workers are at high risk of work stress and burnout, with the Covid-19 reported to have amplified this risk. The Mindfulness-based Social Work and Self-Care programme (MBSWSC) has been found to support cognitive and emotion regulation of social workers, leading to improved stress, burnout, mental health, and well-being. This randomised controlled trial (RCT) aimed to replicate and expand the findings of an earlier RCT of MBSWSC, with a wider group of social work professionals (including managers), by evaluating the effects of MBSWSC (n = 29) versus an active control (n = 31). Replication of RCTs acts as an important means by which findings can be confirmed, results replicated, generalisability assessed and processes and applicability improved. When compared to an online active control group, MBSWSC (which was also delivered online) was found to improve stress, emotional exhaustion, depersonalisation of service users, anxiety, depression, well-being, along with a range of mindfulness mechanisms of action which support cognitive and emotion self-regulation. The results from this study evidence the acceptability, effectiveness and durability of MBSWSC, and provide clear guidance that if MBSWSC is implemented across social work services, social workers are likely to experience improvements in these critical social work practice and self-care outcomes.
Ultradian rhythms in accelerometric and autonomic data vary based on seizure occurrence in paediatric epilepsy patients
Authors: Vieluf Solveig , Cantley Sarah , Krishnan Vaishnav , Loddenkemper Tobias
Published: February 2024

Ultradian rhythms are physiological oscillations that resonate with period lengths shorter than 24 hours. This study examined the expression of ultradian rhythms in patients with epilepsy, a disease defined by an enduring seizure risk that may vary cyclically. Using a wearable device, we recorded heart rate, body temperature, electrodermal activity and limb accelerometry in patients admitted to the paediatric epilepsy monitoring unit. In our case–control design, we included recordings from 29 patients with tonic–clonic seizures and 29 non-seizing controls. We spectrally decomposed each signal to identify cycle lengths of interest and compared average spectral power- and period-related markers between groups. Additionally, we related seizure occurrence to the phase of ultradian rhythm in patients with recorded seizures. We observed prominent 2- and 4-hour-long ultradian rhythms of accelerometry, as well as 4-hour-long oscillations in heart rate. Patients with seizures displayed a higher peak power in the 2-hour accelerometry rhythm (U = 287, P = 0.038) and a period-lengthened 4-hour heart rate rhythm (U = 291.5, P = 0.037). Those that seized also displayed greater mean rhythmic electrodermal activity (U = 261; P = 0.013). Most seizures occurred during the falling-to-trough quarter phase of accelerometric rhythms (13 out of 27, χ2 = 8.41, P = 0.038). Fluctuations in seizure risk or the occurrence of seizures may interrelate with ultradian rhythms of movement and autonomic function. Longitudinal assessments of ultradian patterns in larger patient samples may enable us to understand how such rhythms may improve the temporal precision of seizure forecasting models.
Confined space of a nickel-triazole metal–organic framework responsible for high product selectivity and enantiospecific yield of lactic acid converted from sugar in a water-based system
Authors: Adpakpang Kanyaporn , Ponchai Panyapat , Pukdeejorhor Ladawan , Faungnawakij Kajornsak , Bureekaew Sareeya
Published: February 2024

Enantioselective production of lactic acid from xylose sugar in the constrained pore space of the Ni-triazole metal-organic framework (MOF) is demonstrated. A robust hydrophilic structure with a confined nano-pocket is constructed from the [Ni3(µ3-OH)(Tz)3(OH)2(H2O)4]n secondary building unit (SBU). At elevated temperature, the de-coordination of water and hydroxide species on the Ni node can generate accessible an open metal site (OMS) possessing Lewis acidicity, acting as catalytic center for the catalysis of xylose to lactic acid. Density functional theory (DFT) calculations suggests that an enantiospecific yield of lactic acid is realizable by a preferential interaction between any pair of SBUs and the interlocked trans-pyruvaldehyde intermediate. This work highlights the unique spatial and chemical environment of MOFs as an advantageous platform for specific processes in catalysis.
Asymmetrical cortical surface area decrease in epilepsy patients with postictal generalized electroencephalography suppression
Authors: Zhao Chenyang , Tang Yingying , Xiao Yuan , Jiang Ping , Zhang Ziyi , Gong Qiyong , Zhou Dong
Published: February 2024

Postictal generalized electroencephalographic suppression is a possible electroencephalographic marker for sudden unexpected death in epilepsy. We aimed to investigate the cortical surface area abnormalities in epilepsy patients with postictal generalized electroencephalographic suppression. We retrospectively included 30 epilepsy patients with postictal generalized electroencephalographic suppression (PGES+), 21 epilepsy patients without postictal generalized electroencephalographic suppression (PGES−), and 30 healthy controls. Surface-based analysis on high-resolution T1-weighted images was conducted and cortical surface areas were compared among the three groups, alongside correlation analyses with seizure-related clinical variables. Compared with PGES− group, we identified reduced surface area in the bilateral insula with more extensive distribution in the right hemisphere in PGES+ group. The reduced right insular surface area was associated with younger seizure-onset age. When compared with healthy controls, PGES− group presented reduced surface area in the left caudal middle frontal gyrus; PGES+ group presented more widespread surface area reductions in the right posterior cingulate gyrus, left postcentral gyrus, middle frontal gyrus, and middle temporal gyrus. Our results suggested cortical microstructural impairment in patients with postictal generalized electroencephalographic suppression. The significant surface area reductions in the insular cortex supported the autonomic network involvement in the pathology of postictal generalized electroencephalographic suppression, and its right-sided predominance suggested the potential shared abnormal brain network for postictal generalized electroencephalographic suppression and sudden unexpected death in epilepsy.
Brain activity supporting alternating speech for semantic words: simultaneous magnetoencephalographic recording
Authors: Anada Risa , Watanabe Hayato , Shimojo Atsushi , Shiraishi Hideaki , Yokosawa Koichi
Published: February 2024

Communication, especially conversation, is essential for human social life. Many previous studies have examined the neuroscientific underpinnings of conversation, i.e. language comprehension and speech production. However, conversation inherently involves two or more people, and unless two people actually interact with one another, the nature of the conversation cannot be truly revealed. Therefore, in this study, we used two magnetoencephalographs that were connected together, and simultaneously recorded brain activity while two people took turns speaking in a word association/alphabet completion task. We compared the amplitude modulation of the alpha- and beta-band rhythms within each of the 62 brain regions under semantic (word association; less predictable) and non-semantic (alphabet completion; more predictable) conditions. We found that the amplitudes of the rhythms were significantly different between conditions in a wide range of brain regions. Additionally, significant differences were observed in nearly the same group of brain regions after versus before each utterance, indicating that a wide range of brain areas is involved in predicting a conversation partner’s next utterance. This result supports the idea that mentalizing, e.g. predicting another person's speech, plays an important role in conversation, and suggests that the neural network implicated in mentalizing extends over a wide range of brain regions.
Mapping individual structural covariance network in development brain with dynamic time warping
Authors: Sun Hui , Sun Qinyao , Li Yuanyuan , Zhang Jiang , Xing Haoyang , Wang Jiaojian
Published: February 2024

A conspicuous property of brain development or maturity is coupled with coordinated or synchronized brain structural co-variation. However, there is still a lack of effective approach to map individual structural covariance network. Here, we developed a novel individual structural covariance network method using dynamic time warping algorithm and applied it to delineate developmental trajectories of topological organizations of structural covariance network from childhood to early adulthood with a large sample of 655 individuals from Human Connectome Project-Development dataset. We found that the individual structural covariance network exhibited small-worldness property and the network global topological characteristics including small-worldness, global efficiency, local efficiency, and modularity linearly increase with age while the shortest path length linearly decreases with age. The nodal topological properties including betweenness and degree increased with age in language and emotion regulation related brain areas, while it decreased with age mainly in visual cortex, sensorimotor area, and hippocampus. Moreover, the topological attributes of structural covariance network as features could predict the age of each individual. Taken together, our results demonstrate that dynamic time warping can effectively map individual structural covariance network to uncover the developmental trajectories of network topology, which may facilitate future investigations to establish the links of structural co-variations with respect to cognition and disease vulnerability.
The shorter a man is, the more he defends fairness: relative height disadvantage promoting third-party punishment—evidence from inter-brain synchronization
Authors: Meng Yujia , Jia Shuyu , Liu Jingyue , Zhang Chenyu , Wang He , Liu Yingjie
Published: February 2024

Third-party punishment occurs in interpersonal interactions to sustain social norms, and is strongly influenced by the characteristics of the interacting individuals. During social interactions, height is the striking physical appearance features first observed, height disadvantage may critically influence men’s behavior and mental health. Herein, we explored the influence of height disadvantage on third-party punishment through time-frequency analysis and electroencephalography hyperscanning. Two participants were randomly designated as the recipient and third party after height comparison and instructed to complete third-party punishment task. Compared with when the third party’s height is higher than the recipient’s height, when the third party’s height is lower, the punishment rate and transfer amount were significantly higher. Only for highly unfair offers, the theta power was significantly greater when the third party’s height was lower. The inter-brain synchronization between the recipient and the third party was significantly stronger when the third party’s height was lower. Compared with the fair and medium unfair offers, the inter-brain synchronization was strongest for highly unfair offers. Our findings indicate that the height disadvantage-induced anger and reputation concern promote third-party punishment and inter-brain synchronization. This study enriches research perspective and expands the application of the theory of Napoleon complex.
SEPDB: a database of secreted proteins
Authors: Wang Ruiqing , Ren Chao , Gao Tian , Li Hao , Bo Xiaochen , Zhu Dahai , Zhang Dan , Chen Hebing , Zhang Yong
Published: February 2024

Detecting changes in the dynamics of secreted proteins in serum has been a challenge for proteomics. Enter secreted protein database (SEPDB), an integrated secretory proteomics database offering human, mouse and rat secretory proteomics datasets collected from serum, exosomes and cell culture media. SEPDB compiles secreted protein information from secreted protein database, UniProt and Human Protein Atlas databases to annotate secreted proteomics data based on protein subcellular localization and disease markers. SEPDB integrates the latest predictive modeling techniques to measure deviations in the distribution of signal peptide structures of secreted proteins, extends signal peptide sequence prediction by excluding transmembrane structural domain proteins and updates the validation analysis pipeline for secreted proteins. To establish tissue-specific profiles, we have also created secreted proteomics datasets associated with different human tissues. In addition, we provide information on heterogeneous receptor network organizational relationships, reflective of the complex functional information inherent in the molecular structures of secreted proteins that serve as ligands. Users can take advantage of the Refreshed Search, Analyze, Browse and Download functions of SEPDB, which is available online at https://sysomics.com/SEPDB/.

Database URL:  https://sysomics.com/SEPDB/
Risks and benefits of sharing patient information on social media: a digital dilemma
Authors: van der Boon Robert M A , Camm A John , Aguiar C , Biasin E , Breithardt G , Bueno H , Drossart I , Hoppe N , Kamenjasevic E , Ladeiras-Lopes R , McGreavy Paul , Lanzer P , Vidal-Perez R , Bruining Nico
Published: February 2024

Social media (SoMe) has witnessed remarkable growth and emerged as a dominant method of communication worldwide. Platforms such as Facebook, X (formerly Twitter), LinkedIn, Instagram, TikTok, and YouTube have become important tools of the digital native generation. In the field of medicine, particularly, cardiology, attitudes towards SoMe have shifted, and professionals increasingly utilize it to share scientific findings, network with experts, and enhance teaching and learning. Notably, SoMe is being leveraged for teaching purposes, including the sharing of challenging and intriguing cases. However, sharing patient data, including photos or images, online carries significant implications and risks, potentially compromising individual privacy both online and offline. Privacy and data protection are fundamental rights within European Union treaties, and the General Data Protection Regulation (GDPR) serves as the cornerstone of data protection legislation. The GDPR outlines crucial requirements, such as obtaining ‘consent’ and implementing ‘anonymization’, that must be met before sharing sensitive and patient-identifiable information. Additionally, it is vital to consider the patient’s perspective and prioritize ethical and social considerations when addressing challenges associated with sharing patient information on SoMe platforms. Given the absence of a peer-review process and clear guidelines, we present an initial approach, a code of conduct, and recommendations for the ethical use of SoMe. In conclusion, this comprehensive review underscores the importance of a balanced approach that ensures patient privacy and upholds ethical standards while harnessing the immense potential of SoMe to advance cardiology practice and facilitate knowledge dissemination.
High diversity, abundance, and expression of hydrogenases in groundwater
Authors: Li Shengjie , Mosier Damon , Kouris Angela , Humez Pauline , Mayer Bernhard , Strous Marc , Diao Muhe
Published: February 2024

Hydrogen may be the most important electron donor available in the subsurface. Here we analyse the diversity, abundance and expression of hydrogenases in 5 proteomes, 25 metagenomes, and 265 amplicon datasets of groundwaters with diverse geochemistry. A total of 1545 new [NiFe]-hydrogenase gene sequences were recovered, which considerably increased the number of sequences (1999) in a widely used database. [NiFe]-hydrogenases were highly abundant, as abundant as the DNA-directed RNA polymerase. The abundance of hydrogenase genes increased with depth from 0 to 129 m. Hydrogenases were present in 481 out of 1245 metagenome-assembled genomes. The relative abundance of microbes with hydrogenases accounted for ~50% of the entire community. Hydrogenases were actively expressed, making up as much as 5.9% of methanogen proteomes. Most of the newly discovered diversity of hydrogenases was in “Group 3b”, which has been associated with sulfur metabolism. “Group 3d”, facilitating the interconversion of electrons between hydrogen and NAD, was the most abundant and mainly observed in methanotrophs and chemoautotrophs. “Group 3a”, associated with methanogenesis, was the most abundant in proteomes. Two newly discovered groups of [NiFe]-hydrogenases, observed in Methanobacteriaceae and Anaerolineaceae, further expanded diversity. Our results highlight the vast diversity, abundance and expression of hydrogenases in groundwaters, suggesting a high potential for hydrogen oxidation in subsurface habitats.
Can feature structure improve model’s precision? A novel prediction method using artificial image and image identification
Authors: He Yupeng , Sun Qiwen , Matsunaga Masaaki , Ota Atsuhiko
Published: February 2024

Objectives

This study aimed to develop an approach to enhance the model precision by artificial images.

Materials and Methods

Given an epidemiological study designed to predict 1 response using f features with M samples, each feature was converted into a pixel with certain value. Permutated these pixels into F orders, resulting in F distinct artificial image sample sets. Based on the experience of image recognition techniques, appropriate training images results in higher precision model. In the preliminary experiment, a binary response was predicted by 76 features, the sample set included 223 patients and 1776 healthy controls.

Results

We randomly selected 10 000 artificial sample sets to train the model. Models’ performance (area under the receiver operating characteristic curve values) depicted a bell-shaped distribution.

Conclusion

The model construction strategy developed in the research has potential to capture feature order related information and enhance model predictability.
Evaluation of the Canadian approved drug screening equipment cut-off levels for tetrahydrocannabinol (THC)
Authors: Copley Heather , Filbert Angela , Williams Kali
Published: February 2024

In 2018, Canada introduced roadside oral fluid (OF) screening devices, called Approved Drug Screening Equipment (ADSE), as an investigative tool in impaired driving investigations to detect tetrahydrocannabinol (THC), cocaine and/or methamphetamine in drivers. In this work, we compare the detection and concentration of THC in blood samples collected from suspected impaired drivers that tested positive at the roadside for THC on an ADSE. The two ADSEs that were utilized were the Dräger DrugTest® 5000 (DDT) and the Abbott SoToxa™ (SoToxa), both configured with a THC OF concentration cut-off concentration of 25 ng/mL. Blood samples were screened for cannabinoids using immunoassay and positive results were followed up by confirmation/quantitation of THC by ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS-MS). A total of 230 cases were available where a blood sample was collected from a suspected impaired driver subsequent to a positive THC screen result on an ADSE. The blood samples were taken an average of 1.4 hours (range = 9 minutes to 3.2 hours) after the ADSE test. THC was confirmed in 98% of blood samples with concentrations across all samples ranging from not detected (cut = off 0.5 ng/mL) to greater than 20 ng/mL. Further, 90% of the blood samples had a THC concentration of 2.0 ng/mL (the lower per se limit in Canada) or greater. A positive ADSE test of a suspected impaired driver may predict that the driver has a detectable level of THC in their blood, and there is a high likelihood that the THC blood concentration is 2.0 ng/mL or higher. Hence, ADSE may be a useful tool for law enforcement and aid in the development of grounds to believe that a driver is operating a conveyance with a THC concentration exceeding Canadian per se limits.
Description of a new species of Forcipomyia (Lasiohelea) (Diptera: Ceratopogonidae) and a key to species of the F. (L.) taiwana species group in China
Authors: Zhang Yu Ling , Jiang Xiao Hong , Hou Xiao Hui
Published: February 2024

Forcipomyia (Lasiohelea) (Meigen, Diptera: Ceratopogonidae) penguin sp. nov. is described and illustrated based on male specimens from China. It is characterized by the approximately rectangular aedeagus, which is longitudinally split in the middle, with the apices slightly bending to the outside, making it into the shape of a hook; bilobed aedeagus is very closely connected. The description is provided using scanning electron microscopes, light microscopes, and camera lucida drawing. The specimens were collected from woods near a pond in Liping County, Guizhou Province. We provide both keys to male and female of Forcipomyia (Lasiohelea) taiwana species group in China.
Impact of incidental synucleinopathy in mild cognitive impairment due to Alzheimer disease
Authors: Shriram Jahnavi , Malek-Ahmadi Michael , Irwin Chase , Sabbagh Marwan
Published: February 2024

Recent evidence suggests that the presence of α-synuclein Lewy bodies (LBs) correlates with accelerated disease progression in patients with Alzheimer disease (AD) but it is unclear whether this effect is also exerted in the mild cognitive impairment (MCI) phase of AD. We sought to determine whether incidental LB pathology in patients with MCI due to AD is associated with a faster rate of cognitive decline compared to MCI controls without LB pathology. We identified patients within the National Alzheimer’s Coordinating Center (NACC) database with MCI due to AD and stratified the cohort by the presence or absence of synucleinopathy. We utilized a repeated measures longitudinal analysis of Mini-Mental State Examination (MMSE) scores to determine whether the decline in performance occurred at a greater rate in the synucleinopathy patients. A total of 206 participants were studied; 80 had coincident synucleinopathy. The rate of decline in MMSE scores between the groups did not differ. This may suggest that a synergistic effect of LB and AD neuropathology is only appreciable in the later stages of disease progression. Further investigation into the effect of mixed LB and AD pathology in the early stages of cognitive impairment is warranted to highlight opportunities for targeted early intervention in patients.
How shared ties and journalistic cultures shape global news coverage of disruptive media events: the case of the 9/11 terror attacks
Authors: Jungblut Marc , Althaus Scott , Bajjalieh Joseph , Chan Chung-hong , Welbers Kasper , van Atteveldt Wouter , Wessler Hartmut
Published: February 2024

In recent decades, disruptive media events, such as major terrorist attacks, have gained increasing relevance in news coverage around the world. Despite the growing importance of such globally broadcast media events, little research to date has examined cross-national variation in event coverage or the predictors of this variation. This study examines news coverage about the 9/11 terror attacks in the United States across 51 countries to analyze whether the topical focus and emotional tone of news coverage about the attacks varied according to a country’s proximity to the United States and the dominant role perceptions of its journalistic culture. We show that these macro-level predictors are associated in varying degrees with the country-level topical focus and emotional tone of reporting over the 30 days following this salient event. Moreover, our analysis also suggests that temporal developments may have uniformly structured much of this worldwide coverage.
The one-sayers model for the Extended Crosswise design
Authors: Cruyff Maarten J L F , Sayed Khadiga H A , Petróczi Andrea , van der Heijden Peter G M
Published: February 2024

The Extended Crosswise design is a randomized response design characterized by a sensitive and an innocuous question and two sub-samples with complementary randomization probabilities of the innocuous question. The response categories are ‘One’ with two different answers and ‘Two’ with two answers that are the same. Due to the complementary randomization probabilities, ‘One’ is the incriminating response in one sub-sample, and ‘Two’ in the other. The use of two sub-samples generates a degree of freedom to test for response biases with a goodness-of-fit test, but this test is unable to detect bias resulting from self-protective respondents giving the non-incriminating response when the incriminating response was required. This raises the question what a significant goodness-of-fit test measures? In this paper, we hypothesize that respondents are largely unaware which response is associated with the sensitive characteristic, and intuitively perceive ‘One’ as the safer response. We present empirical evidence for one-saying in six surveys among a total of 4,242 elite athletes, and present estimates of doping use corrected for it. Furthermore, logistic regression analyses are conducted to test the hypothesis that respondents who complete the survey in a short time are more likely to answer randomly, and therefore are less likely to be one-sayers.
Anti-E alloimmunization from a platelet apheresis transfusion in a 22-month-old male with acute myeloid leukemia
Authors: Burks Martin , Warren Christina S , Lightfoot Thomas , Fadeyi Emmanuel A
Published: February 2024

RhD alloimmunization from platelet transfusions have been documented in the literature. However, non-RhD platelet alloimmunization is much less frequent and the risk for non-RhD alloimmunization from platelets is thought to be extremely low and most associated with buffy coat pooled platelets. A 22-month-old male with acute myeloid leukemia received 99 mL apheresis platelets for thrombocytopenia. Three months later, an antibody screen, the direct antiglobulin test (DAT), and red blood cell (RBC) genotype were sent for laboratory evaluation. The antibody screen was positive, with anti-E identified. The DAT was negative and the RBC genotype of the patient was predicted to be negative for the E antigen whereas the platelet donor was predicted to be positive for E antigen. There is a risk of alloimmunization of non-RhD antigen from platelet pheresis transfusion even in a patient less than 2 years old.
Poaceae plants transfer cyclobutane pyrimidine dimer photolyase to chloroplasts for ultraviolet-B resistance
Authors: Otake Momo , Teranishi Mika , Komatsu Chiharu , Hara Mamoru , Yoshiyama Kaoru Okamoto , Hidema Jun
Published: February 2024

Photoreactivation enzyme that repairs cyclobutane pyrimidine dimer (CPD) induced by ultraviolet-B radiation, commonly called CPD photolyase (PHR) is essential for plants living under sunlight. Rice (Oryza sativa) PHR (OsPHR) is a unique triple-targeting protein. The signal sequences required for its translocation to the nucleus or mitochondria are located in the C-terminal region but have yet to be identified for chloroplasts. Here, we identified sequences located in the N-terminal region, including the serine-phosphorylation site at position 7 of OsPHR, and found that OsPHR is transported/localized to chloroplasts via a vesicle transport system under the control of serine-phosphorylation. However, the sequence identified in this study is only conserved in some Poaceae species, and in many other plants, PHR is not localized to the chloroplasts. Therefore, we reasoned that Poaceae species need the ability to repair CPD in the chloroplast genome to survive under sunlight and have uniquely acquired this mechanism for PHR chloroplast translocation.
Abscisic acid root-to-shoot translocation by transporter AtABCG25 mediates stomatal movements in Arabidopsis
Authors: Yang Qin , Deng Xiaojuan , Liu Ting , Qian Jiayun , Zhang Penghong , Zhu Engao , Wang Jingqi , Zhu Xiaoxian , Kudoyarova Guzel , Zhao Jiangzhe , Zhang Kewei
Published: February 2024

The phytohormone abscisic acid (ABA) plays a central role in regulating stomatal movements under drought conditions. The root-derived peptide CLAVATA3/EMBRYO SURROUNDING REGION-RELATED 25 (CLE25) moves from the root to shoot for activating ABA biosynthesis under drought conditions. However, the root-to-shoot translocation of root-derived ABA and its regulation of stomatal movements in the shoot remain to be clarified. Here, we reveal that the ABA transporter ATP-binding cassette subfamily G member 25 (AtABCG25) mediates root-to-shoot translocation of ABA and ABA-glucosyl ester (ABA-GE) in Arabidopsis (Arabidopsis thaliana). Isotope-labeled ABA tracer experiments and hormone quantification in xylem sap showed that the root-to-shoot translocation of ABA and ABA-GE was substantially impaired in the atabcg25 mutant under nondrought and drought conditions. However, the contents of ABA and ABA-GE in the leaves were lower in the atabcg25 mutant than in the wild type (WT) under nondrought but similar under drought conditions. Consistently, the stomatal closure was suppressed in the atabcg25 mutant under nondrought but not under drought conditions. The transporter activity assays showed that AtABCG25 directly exported ABA and ABA-GE in planta and in yeast (Saccharomyces cerevisiae) cells. Thus, we proposed a working model in which root-derived ABA transported by AtABCG25 via xylem mediates stomatal movements in the shoot under nondrought conditions but might exhibit little effect on stomatal movements under drought conditions. These findings extend the functions of AtABCG25 and provide insights into the long-distance translocation of ABA and its role in stomatal movements.
Superstition in Surgery: A Population-Based Cohort Study to Assess the Association Between Surgery on Friday the 13th and Postoperative Outcomes
Authors: Ranganathan Sanjana , Riveros Carlos , Geng Michael , Chang Courtney , Tsugawa Yusuke , Ravi Bheeshma , Melchiode Zachary , Hu Siqi , Kobashi Kathleen , Miles Brian J. , Klaassen Zachary , Nathens Avery , Coburn Natalie , Detsky Allan S. , Jerath Angela , Wallis Christopher J. D. , Satkunasivam Raj
Published: February 2024

Objective:

We sought to examine whether the outcomes of patients who receive a surgical procedure on Friday the 13th differ from patients who receive surgery on flanking Fridays.

Background:

Numerous studies have demonstrated that increased anxiety from the provider or patient around the time of surgery can lead to worse outcomes. Superstitious patients often express significant concern and anxiety when undergoing a surgical procedure on Friday the 13th.

Methods:

A retrospective, population-based cohort study of 19,747 adults undergoing 1 of 25 common surgical procedures on Friday the 13th or flanking control Fridays (Friday the 6th and Friday the 20th) between January 1, 2007, and December 31, 2019, with 1 year of follow-up. The main outcomes included death, readmission, and complications at 30 days (short-term), 90 days (intermediate-term), and 1 year (long-term).

Results:

A total of 7,349 (37.2%) underwent surgery on Friday the 13th, and 12,398 (62.8%) underwent surgery on a flanking Friday during the study period. Patient characteristics were similar between the 2 groups. We found no evidence that patients receiving surgery on Friday the 13th group were more likely to experience the composite primary outcome at 30 days [adjusted odds ratio (aOR) = 1.02 (95% CI = 0.94–1.09)], 90 days [aOR = 0.97 (95% CI = 0.90–1.04)], and 1 year [aOR = 0.99 (95% CI = 0.94–1.04)] after surgery.

Conclusion:

Patients receiving surgery on Friday the 13th do not appear to fare worse than those treated on ordinary Fridays with respect to the composite outcome.
Cell-Free Hemoglobin in the Pathophysiology of Trauma: A Scoping Review
Authors: Ross James T. , Robles Anamaria J. , Mazer Monty B. , Studer Amy C. , Remy Kenneth E. , Callcut Rachael A.
Published: February 2024

OBJECTIVES:

Cell-free hemoglobin (CFH) is a potent mediator of endothelial dysfunction, organ injury, coagulopathy, and immunomodulation in hemolysis. These mechanisms have been demonstrated in patients with sepsis, hemoglobinopathies, and those receiving transfusions. However, less is known about the role of CFH in the pathophysiology of trauma, despite the release of equivalent levels of free hemoglobin.

DATA SOURCES:

Ovid MEDLINE, Embase, Web of Science Core Collection, and BIOSIS Previews were searched up to January 21, 2023, using key terms related to free hemoglobin and trauma.

DATA EXTRACTION:

Two independent reviewers selected studies focused on hemolysis in trauma patients, hemoglobin breakdown products, hemoglobin-mediated injury in trauma, transfusion, sepsis, or therapeutics.

DATA SYNTHESIS:

Data from the selected studies and their references were synthesized into a narrative review.

CONCLUSIONS:

Free hemoglobin likely plays a role in endothelial dysfunction, organ injury, coagulopathy, and immune dysfunction in polytrauma. This is a compelling area of investigation as multiple existing therapeutics effectively block these pathways.
A Retrospective Analysis of Safety in Participants Treated with a Hybrid Hyaluronic Acid and Calcium Hydroxyapatite Filler
Authors: Braz André , Colucci Ligia , Macedo de Oliveira Luciana , Monteiro Grasiela , Ormiga Patricia , Wanick Fabiana , Cazerta Camila , Kerson Graeme , Musumeci Maria , Silberberg Michael
Published: February 2024

Background:

Limited long-term safety data are published on HA/CaHA/L, a hybrid dermal filler combining hyaluronic acid (HA), calcium hydroxyapatite (CaHA), and lidocaine (L).

Methods:

This retrospective multicenter study assessed treatment-emergent adverse events (TEAEs) in adults treated with HA/CaHA/L. The full analysis set (FAS) included eligible consented adults (N = 403); the long-term safety analysis (LTSA) set included FAS participants with greater than or equal to 12-months HA/CaHA/L exposure (n = 243).

Results:

Participants were majority female (94.0%), with Fitzpatrick skin phototypes II/III (80.1%) and a mean age of 50.1 years. Most participants (86.4%) received one HA/CaHA/L treatment. The median time between participants’ first HA/CaHA/L treatment and chart review was 15.4 months. Participants received a mean of 2.2 mL (0.5-8.9 mL) filler per treatment. Treated areas were predominantly malar (71.2%) and mandible (69.7%) regions. Most participants (95.0%) had one or more aesthetic treatments other than HA/CaHA/L [eg, other dermal fillers (84.1%), botulinum toxin (63.3%)]. Nineteen (4.7%) FAS participants had 20 documented TEAEs; most (3.5%, n = 14 participants) were mild in severity. Twelve TEAEs in 11 participants (2.7%) were related to HA/CaHA/L: induration (three, 0.7%), edema (3, 0.7%), and implant site nodules (five, 1.2%), which were noninflammatory and likely related to product placement. Among the LTSA, 15 (6.2%) participants had 16 documented TEAEs (six edema, five implant site nodules, one inflammation, three skin induration, one hypersensitivity); most were mild in severity. Nine TEAEs in eight participants (3.3%) were HA/CaHA/L-related. No treatment-emergent serious AEs were reported.

Conclusion:

The data from this noninterventional retrospective study support the favorable longer term (>12 month) safety profile of HA/CaHA/L.