bluuebunny/crossref_metadata_2025 huggingface dataset:
Interleukin-6 as a Director of Immunological Events and Tissue Regenerative Capacity in Hemodialyzed Diabetes Patients
Authors: Trandafir Maria-Florina , Savu Octavian , Pasarica Daniela , Bleotu Coralia , Gheorghiu Mihaela
Published: June 2024

Hemodialyzed patients have innate immunity activation and adaptive immunity senescence. Diabetes mellitus is a frequent cause for chronic kidney disease and systemic inflammation. We studied the immunological pattern (innate and acquired immunity) and the tissular regeneration capacity in two groups of hemodialyzed patients: one comprised of diabetics and the other of non-diabetics. For inflammation, the following serum markers were determined: interleukin 6 (IL-6), interleukin 1β (IL-1β), tumoral necrosis factor α (TNF-α), IL-6 soluble receptor (sIL-6R), NGAL (human neutrophil gelatinase-associated lipocalin), and interleukin 10 (IL-10). Serum tumoral necrosis factor β (TNF-β) was determined as a cellular immune response marker. Tissue regeneration capacity was studied using neurotrophin-3 (NT-3) and vascular endothelial growth factor β (VEGF-β) serum levels. The results showed important IL-6 and sIL-6R increases in both groups, especially in the diabetic patient group. IL-6 generates trans-signaling at the cellular level through sIL-6R, with proinflammatory and anti-regenerative effects, confirmed through a significant reduction in NT-3 and VEGF-β. Our results suggest that the high serum level of IL-6 significantly influences IL-1β, TNF-β, NT-3, VEGF-β, and IL-10 behavior. Our study is the first that we know of that investigates NT-3 in this patient category. Moreover, we investigated VEGF-β and TNF-β serum behavior, whereas most of the existing data cover only VEGF-α and TNF-α in hemodialyzed patients.
Effect of Bismuth Content and Heating Rate on MnS Inclusions in Free-Cutting Steel
Authors: Xie Chunlu , Zhang Hongmei , Zhao Gang , Li Xiangyu , Li Hongnan , Yu Guoao , Jiang Zhengyi
Published: June 2024

In this paper, the influence of bismuth content and heating rate on the morphology of MnS inclusions in bismuth-containing free-cutting steel during heating was investigated through in situ observation experiments and 3D electrolytic corrosion experiments. By observing the microscopic morphology of inclusions in the original sample, it was found that MnS inclusions in the sample were rod-shaped, spherical, irregular, small in size, and mostly clustered at the grain boundary in the form of chains and divergences. With the addition of bismuth, MnS inclusions of a larger size appear in the steel, and the inclusions distributed at grain boundaries are also reduced. When bismuth (0.010~0.020%) is added to the steel, MnS is mainly spherical and uniformly dispersed in the steel matrix. If the bismuth content is too high, the inclusions aggregate. Through in situ observations of the inclusions in the sample, it was found that the addition of bismuth in the heating process delays the appearance of ferrite grain boundaries and contributes to the spheroidization of MnS inclusions. Mn and S elements can fully diffuse slowly in the matrix with a heating rate below 1 °C/s and a long holding time (300 s), which provides the possibility for the spheroidization of MnS inclusions.
Micro-WEDM of Ti-29Nb-13Ta-4.6Zr Alloy for Antibacterial Properties: Experimental Investigation and Optimization
Authors: Ali Shahid , Omarov Salikh , Utebayeva Altynay , Pham Tri Thanh , Talamona Didier , Perveen Asma
Published: June 2024

Recent developments of orthopedic implant applications have discovered a variety of new metallic biomaterials known as β-type titanium alloys. The μ-WEDM (micro-wire electro discharge machining) surface treatment technique, capable of improving the surface properties of orthopedic implants, was studied in a machining Ti-29Nb-13Ta-4.6Zr alloy. This study aimed to evaluate material removal rate (MRR), kerf width, average surface roughness, microhardness and antibacterial response at different machining parameters which are capacitance (1 nF, 10 nF and 100 nF) and gap voltage (80 V, 95 V and 110 V). The Taguchi method was used to optimize the mentioned output parameters, while ANOVA (analysis of variance) described the significance and contribution of capacitance and gap voltage. Grey relation analysis (GRA) was conducted to perform multiple output optimization. For antibacterial response, cultivations of B. subtilis, E. coli, P. aeruginosa and S. aureus bacteria on treated surfaces for 72 h were performed. As the results, optimal values of MRR, kerf width, crater area, average surface roughness and microhardness were equal to 0.0637 mm3/min, 93.0 μm, 21.8 μm2, 0.348 μm and 442 HV, respectively. Meanwhile, μ-WEDM treatment improved antibacterial properties while the highest antibacterial response was achieved at the lowest average surface roughness resulting in least biofilm formation on treated surfaces.
Microstructure and Mechanical Properties of Ti-6Al-4V In Situ Alloyed with 3 wt% Cr by Laser Powder Bed Fusion
Authors: Goettgens Valerie Sue , Weber Luca , Braun Jakob , Kaserer Lukas , Letofsky-Papst Ilse , Mitsche Stefan , Schimbäck David , Leichtfried Gerhard
Published: June 2024

This work studied the microstructure and mechanical properties of Ti-6Al-4V in situ alloyed with 3 wt% Cr by laser powder bed fusion (LPBF). Specimens with a relative density of 99.14 ± 0.11% were produced, showing keyhole and lack of fusion pores. Due to incomplete mixing of the components during melting, chemical inhomogeneities were observed in the solidified material. The addition of Cr promoted thermal supercooling during solidification and induced a reduction in the primary β grain size in the longitudinal direction and a weakening of the otherwise strong ⟨100⟩β texture, both typical issues for Ti-6Al-4V produced by LPBF. The primary β at first transformed martensitically to α’, but by preheating the substrate plate to 500 °C and cyclically reheating the material by melting subsequent layers, in situ martensite decomposition was achieved, resulting in a fine lamellar α + β microstructure. In addition, the B19 phase was detected in the β matrix, presumably caused by Fe impurities in the Cr powder feedstock. Specimens exhibited a hardness of 402 ± 18 HV10, and an excellent ultimate tensile strength of 1450 ± 22 MPa at an elongation at break of 4.5 ± 0.2%.
On the Enhancement of Material Formability in Hybrid Wire Arc Additive Manufacturing
Authors: Pragana João P. M. , Brito Beatriz , Bragança Ivo M. F. , Silva Carlos M. A. , Martins Paulo A. F.
Published: June 2024

This paper is focused on improving material formability in hybrid wire-arc additive manufacturing comprising metal forming stages to produce small-to-medium batches of customized parts. The methodology involves fabricating wire arc additive manufactured AISI 316L stainless steel parts subjected to mechanical and thermal processing (MTP), followed by microhardness measurements, tensile testing with digital image correlation, as well as microstructure and microscopic observations. Results show that mechanical processing by pre-straining followed by thermal processing by annealing can reduce material hardness and strength, increase ductility, and eliminate anisotropy by recrystallizing the as-built dendritic-based columnar grain microstructure into an equiaxed grain microstructure.
Low-Cycle Corrosion Fatigue Deformation Mechanism for an α+β Ti-6Al-4V-0.55Fe Alloy
Authors: Sun Yangyang , Qian Shenwei , Chang Hui , Feng Liang , Li Feng , Zhou Lian
Published: June 2024

Titanium alloys with high strength and good corrosion resistance have become one of the critical bearing structural materials in marine engineering. But in service, corrosion fatigue would occur under the synergetic action of cyclic external load and corrosion environment, threatening the safety of components. In this study, compared with low-cycle fatigue in laboratory air, the low-cycle corrosion fatigue deformation mechanism and fracture characteristic of the Ti-6Al-4V-0.55Fe alloy were investigated in 3.5% NaCl corrosion solution under selected stress amplitudes. The results showed that under low stress amplitude, corrosion fatigue was determined by fatigue damage and corrosion damage, causing a reduction in fatigue life. The local stress concentration caused by corrosion pits and dislocations pile-up accelerated the initiation of fatigue cracks, and other corrosion behavior including crevice corrosion promoted fatigue crack propagation; the corrosion solution increased the surface damage. While under high stress amplitude, due to the short contact time between the sample and solution and higher applied stress, the fatigue life is determined by fatigue damage caused by multiple slips.
Temperature Dependency of Modified Mohr–Coulomb Criterion Parameters for Advanced High Strength Dual-Phase Steel DP780
Authors: Li Yukuan , Li Di , Song Hui , Wang Yiqun , Wu Dongze
Published: June 2024

The Modified Mohr–Coulomb criterion has been demonstrated to exhibit high accuracy in the prediction of fracture in high-strength steels. Taking DP780 as the research object, the undetermined parameters of the Modified Mohr–Coulomb criterion at different temperatures were calibrated by tensile and shear tests combined with simulation. The relationships between the parameters and temperature were investigated. Finally, the relationship between criterion parameters and temperature was verified using the stretch-bending tests of U-shape parts. The fracture of automotive parts can be accurately predicted by simulation during warm stamping, thereby guiding actual production.
Metabolic Insight into Glioma Heterogeneity: Mapping Whole Exome Sequencing to In Vivo Imaging with Stereotactic Localization and Deep Learning
Authors: Servati Mahsa , Vaccaro Courtney N. , Diller Emily E. , Pellegrino Da Silva Renata , Mafra Fernanda , Cao Sha , Stanley Katherine B. , Cohen-Gadol Aaron A. , Parker Jason G.
Published: June 2024

Intratumoral heterogeneity (ITH) complicates the diagnosis and treatment of glioma, partly due to the diverse metabolic profiles driven by underlying genomic alterations. While multiparametric imaging enhances the characterization of ITH by capturing both spatial and functional variations, it falls short in directly assessing the metabolic activities that underpin these phenotypic differences. This gap stems from the challenge of integrating easily accessible, colocated pathology and detailed genomic data with metabolic insights. This study presents a multifaceted approach combining stereotactic biopsy with standard clinical open-craniotomy for sample collection, voxel-wise analysis of MR images, regression-based GAM, and whole-exome sequencing. This work aims to demonstrate the potential of machine learning algorithms to predict variations in cellular and molecular tumor characteristics. This retrospective study enrolled ten treatment-naïve patients with radiologically confirmed glioma. Each patient underwent a multiparametric MR scan (T1W, T1W-CE, T2W, T2W-FLAIR, DWI) prior to surgery. During standard craniotomy, at least 1 stereotactic biopsy was collected from each patient, with screenshots of the sample locations saved for spatial registration to pre-surgical MR data. Whole-exome sequencing was performed on flash-frozen tumor samples, prioritizing the signatures of five glioma-related genes: IDH1, TP53, EGFR, PIK3CA, and NF1. Regression was implemented with a GAM using a univariate shape function for each predictor. Standard receiver operating characteristic (ROC) analyses were used to evaluate detection, with AUC (area under curve) calculated for each gene target and MR contrast combination. Mean AUC for five gene targets and 31 MR contrast combinations was 0.75 ± 0.11; individual AUCs were as high as 0.96 for both IDH1 and TP53 with T2W-FLAIR and ADC, and 0.99 for EGFR with T2W and ADC. These results suggest the possibility of predicting exome-wide mutation events from noninvasive, in vivo imaging by combining stereotactic localization of glioma samples and a semi-parametric deep learning method. The genomic alterations identified, particularly in IDH1, TP53, EGFR, PIK3CA, and NF1, are known to play pivotal roles in metabolic pathways driving glioma heterogeneity. Our methodology, therefore, indirectly sheds light on the metabolic landscape of glioma through the lens of these critical genomic markers, suggesting a complex interplay between tumor genomics and metabolism. This approach holds potential for refining targeted therapy by better addressing the genomic heterogeneity of glioma tumors.
Serum Metabolomic and Lipidomic Profiling Reveals the Signature for Postoperative Obesity among Adult-Onset Craniopharyngioma
Authors: Zhang Qiongyue , Feng Yonghao , Wu Dou , Xie Yinyin , Wu Guoming , Wu Wei , Wang Hui , Liu Xiaoyu , Fan Linling , Xiang Boni , Sun Quanya , Li Yiming , Wang Yongfei , Ye Hongying
Published: June 2024

Craniopharyngioma patients often suffer from a diminished quality of life after surgery, which is usually associated with metabolic disorders and hypothalamic obesity. However, the precise etiology of these conditions remains elusive. To identify the metabolic changes after surgery, we conducted a cross-sectional study using metabolomic and lipidomic analysis to profile metabolic alterations in adult-onset craniopharyngioma patients with postoperative obesity. A cohort of 120 craniopharyngioma patients who had undergone surgery were examined. Differential analyses, including clinical characteristics, serum metabolome, and lipidome, were conducted across distinct body mass index (BMI) groups. Our findings indicated no statistically significant differences in age, sex, and fasting blood glucose among postoperative craniopharyngioma patients when stratified by BMI. However, a noteworthy difference was observed in uric acid and blood lipid levels. Further investigation revealed that alterations in metabolites and lipids were evidently correlated with increased BMI, indicating that postoperative obesity of craniopharyngioma patients affected their whole-body metabolism. Additionally, the multi-omics analysis identified specific metabolites and lipids, including uric acid and DG(18:2/20:4), as contributors to the metabolic disorders associated with postoperative obesity of craniopharyngioma patients. This work provides valuable insight into the involvement of metabolites and lipids in metabolic disorders subsequent to craniopharyngioma surgery.
Rapid Fabrication of Tungsten Oxide-Based Electrochromic Devices through Femtosecond Laser Processing
Authors: Wang Liqun , Zhai Zihao , Li Longnan
Published: June 2024

The sol-gel method is a widely adopted technique for the preparation of tungsten trioxide (WO3) materials, favored for its cost-effectiveness and straightforward production procedures. However, this method encounters challenges such as prolonged annealing periods and limited flexibility in fabricating patterned WO3 films. This study introduces a novel approach that integrates femtosecond laser processing with the sol-gel method to enhance the fabrication of WO3 films. By adjusting polyvinylpyrrolidone (PVP) concentrations during sol-gel synthesis, precise control over film thickness and optimized film properties were achieved. The innovative technique significantly reduced the annealing time required to achieve an 80% transmittance rate from 90 min to 40 min, marking a 56% decrease. Laser processing increased the surface roughness of the films from Sa = 0.032 to Sa = 0.119, facilitating enhanced volatilization of organics during heat treatment. Additionally, this method improved the transmittance modulation of the films by 22% at 550 nm compared to unprocessed counterparts. This approach not only simplifies the manufacturing process but also enhances the optical efficiency of electrochromic devices, potentially leading to broader applications and more effective energy conservation strategies.
Design and Evaluation of an Eye Mountable AutoDALK Robot for Deep Anterior Lamellar Keratoplasty
Authors: Opfermann Justin D. , Wang Yaning , Kaluna James , Suzuki Kensei , Gensheimer William , Krieger Axel , Kang Jin U.
Published: June 2024

Partial-thickness corneal transplants using a deep anterior lamellar keratoplasty (DALK) approach has demonstrated better patient outcomes than a full-thickness cornea transplant. However, despite better clinical outcomes from the DALK procedure, adoption of the technique has been limited because the accurate insertion of the needle into the deep stroma remains technically challenging. In this work, we present a novel hands-free eye mountable robot for automatic needle placement in the cornea, AutoDALK, that has the potential to simplify this critical step in the DALK procedure. The system integrates dual light-weight linear piezo motors, an OCT A-scan distance sensor, and a vacuum trephine-inspired design to enable the safe, consistent, and controllable insertion of a needle into the cornea for the pneumodissection of the anterior cornea from the deep posterior cornea and Descemet’s membrane. AutoDALK was designed with feedback from expert corneal surgeons and performance was evaluated by finite element analysis simulation, benchtop testing, and ex vivo experiments to demonstrate the feasibility of the system for clinical applications. The mean open-loop positional deviation was 9.39 µm, while the system repeatability and accuracy were 39.48 µm and 43.18 µm, respectively. The maximum combined thrust of the system was found to be 1.72 N, which exceeds the clinical penetration force of the cornea. In a head-to-head ex vivo comparison against an expert surgeon using a freehand approach, AutoDALK achieved more consistent needle depth, which resulted in fewer perforations of Descemet’s membrane and significantly deeper pneumodissection of the stromal tissue. The results of this study indicate that robotic needle insertion has the potential to simplify the most challenging task of the DALK procedure, enable more consistent surgical outcomes for patients, and standardize partial-thickness corneal transplants as the gold standard of care if demonstrated to be more safe and more effective than penetrating keratoplasty.
3D-Printed Microdevices: From Design to Applications
Authors: Kalinke Cristiane , Muñoz Rodrigo A. A.
Published: June 2024

3D printing represents an emerging technology in several fields, including engineering, medicine, and chemistry [...]
Viscosity Modeling for Blood and Blood Analog Fluids in Narrow Gap and High Reynolds Numbers Flows
Authors: Knüppel Finn , Malchow Sasha , Sun Ang , Hussong Jeanette , Hartmann Alexander , Wurm Frank-Hendrik , Torner Benjamin
Published: June 2024

For the optimization of ventricular assist devices (VADs), flow simulations are crucial. Typically, these simulations assume single-phase flow to represent blood flow. However, blood consists of plasma and blood cells, making it a multiphase flow. Cell migration in such flows leads to a heterogeneous cell distribution, significantly impacting flow dynamics, especially in narrow gaps of less than 300 μm found in VADs. In these areas, cells migrate away from the walls, forming a cell-free layer, a phenomenon not usually considered in current VAD simulations. This paper addresses this gap by introducing a viscosity model that accounts for cell migration in microchannels under VAD-relevant conditions. The model is based on local particle distributions measured in a microchannels with a blood analog fluid. We developed a local viscosity distribution for flows with particles/cells and a cell-free layer, applicable to both blood and analog fluids, with particle volume fractions of up to 5%, gap heights of 150 μm, and Reynolds numbers around 100. The model was validated by comparing simulation results with experimental data of blood and blood analog fluid flow on wall shear stresses and pressure losses, showing strong agreement. This model improves the accuracy of simulations by considering local viscosity changes rather than assuming a single-phase fluid. Future developments will extend the model to physiological volume fractions up to 40%.
Performance-Enhanced Piezoelectric Micromachined Ultrasonic Transducers by PDMS Acoustic Lens Design
Authors: Jia Licheng , Liang Yong , Meng Fansheng , Zhang Guojun , Wang Renxin , He Changde , Yang Yuhua , Cui Jiangong , Zhang Wendong , Wu Guoqiang
Published: June 2024

This paper delves into enhancing the performance of ScAlN-based Piezoelectric Micromachined Ultrasonic Transducers (PMUTs) through the implementation of Polydimethylsiloxane (PDMS) acoustic lenses. The PMUT, encapsulated in PDMS, underwent thorough characterization through the utilization of an industry-standard hydrophone calibration instrument. The experimental results showed that the ScAlN-based PMUT with the PDMS lenses achieved an impressive sensitivity of −160 dB (re: 1 V/μPa), an improvement of more than 8 dB compared to the PMUT with an equivalent PDMS film. There was a noticeable improvement in the −3 dB main lobe width within the frequency response when comparing the PMUT with PDMS encapsulation, both with and without lenses. The successful fabrication of high-performance PDMS lenses proved instrumental in significantly boosting the sensitivity of the PMUT. Comprehensive performance evaluations underscored that the designed PMUT in this investigation surpassed its counterparts reported in the literature and commercially available transducers. This encouraging outcome emphasizes its substantial potential for commercial applications.
Innovative Stacked Yellow and Blue Mini-LED Chip for White Lamp Applications
Authors: Lee Tzu-Yi , Huang Chien-Chi , Miao Wen-Chien , Hsiao Fu-He , Tsai Chia-Hung , Hung Yu-Ying , Chen Fang-Chung , Lin Chun-Liang , Ohkawa Kazuhiro , He Jr-Hau , Hong Yu-Heng , Kuo Hao-Chung
Published: June 2024

This study introduces a novel approach for fabricating vertically stacked mini-LED arrays, integrating InGaN yellow and blue epitaxial layers with a stress buffer layer to enhance optoelectronic characteristics and structural stability. This method significantly simplifies the LED design by reducing the need for RGB configurations, thus lowering costs and system complexity. Employing vertical stacking integration technology, the design achieves high-density, efficient white light production suitable for multifunctional applications, including automotive lighting and outdoor signage. Experimental results demonstrate the exceptional performance of the stacked yellow and blue mini-LEDs in terms of luminous efficiency, wavelength precision, and thermal stability. The study also explores the performance of these LEDs under varying temperature conditions and their long-term reliability, indicating that InGaN-based yellow LEDs offer superior performance over traditional AlGaInP yellow LEDs, particularly in high-temperature environments. This technology promises significant advancements in the design and application of lighting systems, with potential implications for both automotive and general illumination markets.
Quantification of HPV16 E7 Oncoproteins in Urine Specimens from Women with Cervical Intraepithelial Neoplasia
Authors: Makioka Daiki , Inada Mikio , Awano Masayuki , Saito Ema , Shinoda Takuya , Abe Satoko , Yoshimura Teruki , Müller Martin , Sasagawa Toshiyuki , Ito Etsuro
Published: June 2024

We present the validity of using an ultrasensitive enzyme-linked immunosorbent assay (ELISA) for quantifying high-risk human papillomavirus (HPV) 16 E7 oncoproteins in urine specimens as a noninvasive method of analyzing the oncogenic activity of HPV. Some reports claim that the oncogenic activity of HPV is a more relevant clinical indicator than the presence of HPV DNA for estimating malignant potential. In the present study, urine containing HPV16 and related types were selected by uniplex E6/E7 polymerase chain reaction and classified according to the pathologic diagnosis of cervical intraepithelial neoplasia (CIN) in cervical biopsy specimens. Our ultrasensitive ELISA was able to detect attomole levels of HPV16 E7 oncoproteins, and it detected HPV16-positive SiHa cells at >500 cells/mL without detecting HPV18-positive cells. Our ELISA results showed E7 oncoproteins in 80% (4/5) of urine specimens from women with HPV16-positive CIN1, 71% (5/7) of urine specimens from CIN2 patients, and 38% (3/8) of urine specimens from CIN3 patients. Some urine specimens with undetectable E7 oncoproteins were thought to be negative for live HPV 16-positive cells or in an inactivated state of infection. These results provide the basis for assessing oncogenic activity by quantifying E7 oncoproteins in patient urine.
Hepatitis B Virus Prevalence among HIV-Uninfected People Living in Rural and Peri-Urban Areas in Botswana
Authors: Anderson Motswedi , Mangogola Thabo , Phinius Bonolo B. , Mpebe Gorata , Aimakhu Christopher O. , Choga Wonderful T. , Phakedi Basetsana , Bhebhe Lynnette N. , Ditshwanelo Doreen , Baruti Kabo , Mpofu-Dobo Linda , Othusitse Lebogang , Ratsoma Tsholofelo , Gaolathe Tendani , Makhema Joseph , Shapiro Roger , Lockman Shahin , Moyo Sikhulile , Gaseitsiwe Simani
Published: June 2024

(1) Background: we determined the prevalence of the hepatitis B virus (HBV) amongst people without human immunodeficiency virus (HIV) in rural and peri-urban areas in Botswana. (2) Methods: We screened for the hepatitis B surface antigen (HBsAg) from archived plasma samples of people without HIV (n = 2135) randomly selected from the Botswana Combination Prevention Program (BCPP) (2013–2018). We sequenced 415 bp of the surface region using BigDye sequencing chemistry. (3) Results: The median age of participants was 31 (IQR: 24–46) and 64% (1360/2135) were female. HBV prevalence was 4.0% (86/2135) [95% CI: 3.3–4.9]) and ranged between 0–9.2%. Older participants (>35 years) had increased odds of HBV positivity (OR: 1.94; 95% CI: [1.32–2.86]; p = 0.001). Thirteen samples were sequenced and seven (53.8%) were genotype A, three (23.1%) were genotype D and genotype E each. Clinically significant mutations were identified in the surface region, but no classic drug resistance mutations were identified. (4) Conclusions: We report an HBV prevalence of 4.0% (95% CI 3.3–4.9) among people without HIV in rural and peri-urban communities in Botswana with varying rates in different communities. A comprehensive national HBV program is required in Botswana to guide HBV prevention, testing and management.
Effect of Bacterial Extracellular Polymeric Substances from Enterobacter spp. on Rice Growth under Abiotic Stress and Transcriptomic Analysis
Authors: Aoudi Yosra , Agake Shin-ichiro , Habibi Safiullah , Stacey Gary , Yasuda Michiko , Ohkama-Ohtsu Naoko
Published: June 2024

Plant biostimulants have received attention as sustainable alternatives to chemical fertilizers. Extracellular polymeric substances (EPSs), among the compounds secreted by plant growth-promoting rhizobacteria (PGPRs), are assumed to alleviate abiotic stress. This study aims to investigate the effect of purified EPSs on rice under abiotic stress and analyze their mechanisms. A pot experiment was conducted to elucidate the effects of inoculating EPSs purified from PGPRs that increase biofilm production in the presence of sugar on rice growth in heat-stress conditions. Since all EPSs showed improvement in SPAD after the stress, Enterobacter ludwigii, which was not characterized as showing higher PGP bioactivities such as phytohormone production, nitrogen fixation, and phosphorus solubilization, was selected for further analysis. RNA extracted from the embryos of germinating seeds at 24 h post-treatment with EPSs or water was used for transcriptome analysis. The RNA-seq analysis revealed 215 differentially expressed genes (DEGs) identified in rice seeds, including 139 up-regulated and 76 down-regulated genes. A gene ontology (GO) enrichment analysis showed that the enriched GO terms are mainly associated with the ROS scavenging processes, detoxification pathways, and response to oxidative stress. For example, the expression of the gene encoding OsAAO5, which is known to function in detoxifying oxidative stress, was two times increased by EPS treatment. Moreover, EPS application improved SPAD and dry weights of shoot and root by 90%, 14%, and 27%, respectively, under drought stress and increased SPAD by 59% under salt stress. It indicates that bacterial EPSs improved plant growth under abiotic stresses. Based on our results, we consider that EPSs purified from Enterobacter ludwigii can be used to develop biostimulants for rice.
Assessing PCR-Positive Acanthamoeba Keratitis—A Retrospective Chart Review
Authors: Blaser Frank , Bajka Anahita , Grimm Felix , Metzler Simone , Herrmann Didier , Barthelmes Daniel , Zweifel Sandrine Anne , Said Sadiq
Published: June 2024

Ophthalmologists’ diagnostic and treatment competence in Acanthamoeba keratitis varies widely. This investigator-initiated, retrospective, single-center chart review examined the electronic patient files regarding PCR-positive Acanthamoeba keratitis. We included corneal and contact lens assessments. We further reviewed the patient’s medical history, corneal scraping results regarding viral or fungal co-infections, and the duration from symptom onset to final diagnosis. We identified 59 eyes of 52 patients from February 2010 to February 2023, with 31 of 52 (59.6%) being female patients. The median (IQR, range) patient age was 33 (25.3 to 45.5 [13 to 90]) years, and the mean (SD, range) time to diagnosis after symptom onset was 18 (10.5 to 35 [3 to 70]) days. Overall, 7 of 52 (7.7%) patients displayed a bilateral Acanthamoeba infection, and 48 (92.3%) used contact lenses at symptom onset. Regarding other microbiological co-infections, we found virologic PCR testing in 45 of 52 (86.5%) patients, with 3 (6.7%) positive corneal scrapings. Fungal cultures were performed in 49 of 52 (94.2%) patients, with 5 (10.2%) positive corneal scrapings. The medical treatment success rate was 45/46 (97.8%). This study raises awareness of patient education in contact lens handling and screens for further microbial co-infections in suspected Acanthamoeba cases.
Tuberculosis in a Migrant Population: Integrated Management of a Case through the Prevention Department and Hospital Services
Authors: Fiorito Nahuel , Piacentini Daniela , Cian Serena , Voltolini Anna , Fagherazzi Jacopo , Bino Erica , Brancher Marika , De Luca Giorgia , Battistin Marica , Manzi Mattia , Marcotrigiano Vincenzo , Vedana Angela , Napoli Christian , Cinquetti Sandro
Published: June 2024

Among numerous public health actions, the Prevention Departments of Local Health Authorities take charge of the migrant asylum-seeking population for health assessments, for the implementation of preventive activities, and for any consequent actions. This report describes two cases of tuberculosis in Belluno Province managed by a multidisciplinary team made up of healthcare workers that involved numerous diagnostic, clinical, and prophylactic implications, as well as an analysis of the epidemiological aspects related to the incidence of cases along the migration route. Although the cases occurred in a northeastern Italian territory, the management methods described here may represent good practices to share on this operational line, which can promote the strengthening of cooperation between Health Authorities and Emergency Reception Centers to correctly identify cases of active tuberculosis that may not have been initially screen-detected.
Mineralogy and Selenium Speciation Analysis of Early Cambrian Selenium-Rich Black Shale in Southern Shaanxi Province, China
Authors: Feng Caixia , Liu Shen , Song Wenlei , Hou Chenhui , Yang Yanhong
Published: June 2024

Selenium (Se) is an essential trace element for humans and animals, and an excess of or deficiency in Se is harmful to health. Research on the selenium enrichment zone began in the late 1970s in Shuang’an, Ziyang, southern Shaanxi Province. Naore village is only one selenosis area in Shuang’an, Ziyang, China. Different scholars have conducted systematic studies on the occurrence of selenium, its organic geochemistry and biomarkers, and its content and enrichment patterns in this area. This study applied the TIMA (TESCAN integrated mineral analyzer) for the first time to conduct detailed mineralogical work. The minerals included quartz, carbonate minerals (calcite and dolomite), feldspar (plagioclase, albite, and orthoclase), biotite and muscovite, clay minerals (chlorite and kaolinite), hematite, pyrite, and accessory minerals (almandine, olivine, zircon, and apatite) in Naore village, Ziyang, Shaanxi Province. The ATi index (100 × apatite/(apatite + tourmaline)) is used to determine the source of heavy minerals and the degree of heavy minerals’ weathering. The content POS (100 × (pyroxene + olivine + spinel)/transparent heavy mineral) of olivine, pyroxene, and spinel in heavy minerals can reflect the contribution of basic and ultrabasic rocks in the source area. The ATi and POS indexes for the heavy minerals in the research area were 91.83~99.96 and 0.01~18.75, respectively, reflecting the abundance of volcanic rock material in their source. In addition, the migration, transformation, bioavailability, and toxicity of selenium in the environment are closely related to its species. The species of selenium in various selenium-rich areas (Naore, Wamiao, and Guanquan) mainly include unusable residues and organic forms, followed by humic-acid-bound selenium. The proportions of water-soluble, exchangeable, and carbonate-bound selenium are relatively small, and the proportion of Fe-Mn oxide-bound selenium is the lowest.
Clay Minerals and Biopolymers in Film Design: Overview of Properties and Applications
Authors: Trigueiro Pollyana , Pereira Juliane P. de L. , Ferreira Mirelly G. , Silva Lucas B. , Neves Luan , Peña-Garcia Ramón R.
Published: June 2024

Research to replace petroleum-based plastics has been quite challenging. Currently, there is a lot of interest in biopolymers as an alternative. However, biopolymers do not have suitable mechanical properties when in film form, which limits their applications. To resolve this issue, clay minerals are being incorporated as a strategy. Clay minerals offer the films good barrier, thermal, rheological, optical, and mechanical properties. They can also work with other additives to promote antioxidant and antimicrobial activity. This brief review focuses on incorporating clay minerals with other nanofillers and bioactives to improve their physical, chemical, and functional characteristics. The synergy of these materials gives the films exceptional properties and makes them suitable for applications such as food coatings, packaging materials, dressings, and bandages for treating skin wounds.
Low-Temperature Thermochronology Records the Convergence between the Anatolide–Tauride Block and the Arabian Platform along the Southeast Anatolian Orogenic Belt
Authors: Gildir Semih , Karaoğlan Fatih , Gülyüz Erhan
Published: June 2024

SE Anatolia is witnessing the final stage of the Wilson Cycle, where a continental collision between the Tauride–Anatolide block and Arabian platform occurred, and a 1.5 km Eastern Tauride mountain chain formed. We present new low-temperature thermochronology (LTT) ages, including eight apatite fission track (AFT) and seven apatite and zircon U-Th-Sm/He (AHe, ZHe) ages, for the metamorphic rocks from the Nappe Zone of the Southeast Anatolian Orogenic Belt. The ZHe ages vary from 51.2 ± 0.7 Ma to 30.4 ± 0.6 Ma, the AFT ages range from 33.1 ± 1.6 Ma to 18.1 ± 0.9 Ma, and the AHe ages range from 23.6 ± 2.5 Ma to 6 ± 1.9 Ma. The LTT data show a continuous slow uplift of the region. However, the thermal modeling results suggest an Eocene and middle–late Miocene fast uplift of the region. Similar to our results, the LTT studies along the SAOB show that the vertical movements initiated during the Eocene period have continued in a steady-state regime to recent times. The Eocene epoch is identified by arc–back-arc setting in the region, whereas the Miocene epoch is marked by the continental collision. Within this tectonic framework, vertical movements on the overriding plate are controlled by both extensional and compressional tectonics. The LTT data obtained along the SAOB show fingerprints of thrust propagation from north to south.
Study of Pore–Throat Structure in Tight Triassic Sandstone: A Case Study on the Late Triassic Yanchang Formation, Southwestern Ordos Basin, China
Authors: Xiao Hui , Wang Haonan , Ning Yao , Ma Xiaoli , Yin Shuai
Published: June 2024

In order to better understand pore–throat structure characteristics, the coupling relationship between micropore–throat structure and macro reservoir quality and influencing factors caused by authigenic minerals were studied. Petrographic analyses, scanning electron microscopy (SEM), pressure-controlled mercury injection (PMI), nuclear magnetic resonance (NMR), and X-ray diffraction (XRD) were performed on a suite of tight reservoir samples from the Chang 8 Member of the Upper Triassic Yanchang Formation in the southwestern Ordos Basin, China. The results show that the pore–throat sizes obtained with the combination of PMI and NMR methods varied from nano- to microscale, revealing pore–throat sizes ranging from 0.001 μm to 70 μm, and showing that pore–throats with a radius larger than 1.0 μm are rare. Larger pore–throats with good connectivity (>rapex) account for a smaller part of the total pore volume, ranging from approximately 7.58% to 38.90% with an average of 22.77%, but account for more than 80% of contributions to permeability. The effective movable fluid porosity (φemp) measured by NMR, ranging from approximately 0.10% to 7.07% with an average of 2.56%, had a positive contribution to permeability. The contents of chlorite occurrence state, other than illite, are beneficial to pore–throat preservation. A new reservoir evaluation scheme of the Chang 8 reservoir is established. These research results provide a theoretical basis for the evaluation and development of tight sandstone oil and gas exploration.
The Application of Geosynthetics in Tailings Storage Facilities: A General Review
Authors: Rowe Ronald Kerry , Fan Jiying
Published: June 2024

This paper is a summary of many of the key findings on the application of geosynthetics in tailings storage facilities. Topics include the compressibility and permeability of tailings, the equations predicting leakage through circular and non-circular geomembrane holes, the effect of the subgrade permeability, and the effect of a lateral drainage system within tailings on leakage predictions. Two commonly encountered engineering problems relating to the piping through circular geomembrane holes and the opening width of non-circular defective geomembrane seams are given to demonstrate the potential application of leakage prediction equations. Meanwhile, issues related to the subgrade imperfection and the long-term performance of both high-density polyethylene (HDPE) and bituminous geomembranes in tailings storage applications are addressed. The research highlights that an appropriate HDPE geomembrane liner can be expected to perform very well for an extremely long time, limiting leakage and contaminant migration from the facility into the surrounding environment if the liner is well constructed on a suitable subgrade.
Exploring the Potential of Endophytic Microorganisms and Nanoparticles for Enhanced Water Remediation
Authors: Manganyi Madira Coutlyne , Dikobe Tshegofatso Bridget , Maseme Mametsi Rahab
Published: June 2024

Endophytic microorganisms contribute significantly to water bioremediation by enhancing pollutant degradation and supporting aquatic plant health and resilience by releasing bioactive compounds and enzymes. These microorganisms inhabit plant tissues without causing disease or any noticeable symptoms. Endophytes effectively aid in eliminating contaminants from water systems. Nanoparticles serve as potent enhancers in bioremediation processes, augmenting the efficiency of pollutant degradation by increasing surface area and bioavailability, thereby improving the efficacy and rate of remediation. Their controlled nutrient release and ability to stabilize endophytic colonization further contribute to the enhanced and sustainable elimination of contaminated environments. The synergistic effect of endophytes and nanoparticles in water remediation has been widely explored in recent studies, revealing compelling outcomes. Water pollution poses significant threats to human health, ecosystems, and economies; hence, the sixth global goal of the Sustainable Development Agenda 2030 of the United Nations aims to ensure the availability and sustainable management of water resources, recognizing their crucial importance for current and future generations. Conventional methods for addressing water pollution exhibit several limitations, including high costs, energy-intensive processes, the production of hazardous by-products, and insufficient effectiveness in mitigating emerging pollutants such as pharmaceuticals and microplastics. Noticeably, there is an inability to effectively remove various types of pollutants, thus resulting in incomplete purification cycles. Nanoparticle-enhanced water bioremediation offers an innovative, eco-friendly alternative for degrading contaminants. A growing body of research has shown that integrating endophytic microorganisms with nanoparticles for water bioremediation is a potent and viable alternative. This review examines the potential of using endophytic microorganisms and nanoparticles to enhance water remediation, exploring their combined effects and applications in water purification. The paper also provides an overview of synthetic methods for producing endophyte–nanoparticle composites to optimize their remediation capabilities in aqueous environments. The final section of the review highlights the constraints related to integrating endophytes with nanoparticles.
Switchable Site-Selective Benzanilide C(sp2)-H Bromination via Promoter Regulation
Authors: Sun Yonghui , He Qiyu , Lv Xucheng , Zhang Naizhen , Yan Wei , Sun Jianghui , Zhuang Lili
Published: June 2024

Regioselective benzanilide bromination that generates either regioisomer from the same starting material is desirable. Herein, we develop switchable site-selective C(sp2)-H bromination by promoter regulation. This protocol leads to regiodivergent brominated benzanilide starting from the single substrate via selection of promoters. The protocol demonstrates excellent regioselectivity and good tolerance of functional groups with high yields. The utility effectiveness of this method has been well exemplified in the late-stage modification of biologically important molecules.
Measurement of Zinc Ions in Seawater Samples Using a Microfluidic System Based on the GR/CeO2/Nafion Material
Authors: Tao Wei , Zeng Zexi , Qiu Chengjun , Qu Wei , Zhuang Yuan , Gu Yang , Hao Huili , Zhao Zizi
Published: June 2024

Considering that heavy-metal contamination of seawater is getting worse, building a quick, accurate and portable device for detecting trace zinc in seawater in real time would be very beneficial. In this work, a microfluidic system was developed that includes a planar disc electrode, a micro-cavity for detection, an electrochemical workstation, a computer, a container for waste liquid reprocessing, an external pipeline and other components as well as a graphene/cerium oxide/nano-cerium oxide/Nafion composite membrane was used to modify the planar disc electrode (GR/CeO2/Nafion/Au) to investigate the electrochemical behaviour of Zn(II) using cyclic voltammetry, square-wave voltammetry and orthogonal test methods. Under optimal experimental conditions, the peak reaction current of Zn(II) showed a good linear relationship with the concentration of Zn(II) in the range of 1–900 μg/L with a correlation coefficient of 0.998, and the detection limit of the method was 0.87 μg/L. In addition, the microfluidic system had good stability, reproducibility and anti-interference. The system was used for determining zinc ions in real seawater samples, and the results were very similar to those of inductively coupled plasma–emission spectrometry, demonstrating the practicality of the system for the detection of trace zinc.
Insight into the Discriminative Efficiencies and Mechanisms of Peroxy Activation via Fe/Cu Bimetallic Catalysts for Wastewater Purification
Authors: Xu Tingjin , Fan Lu , Xiong Zhaokun , Lai Bo
Published: June 2024

Fe/Cu bimetallic catalysts have a synergistic effect that can effectively enhance catalytic activity, so Fe/Cu bimetallic catalysts have been extensively studied. However, the efficacy and mechanisms of Fe/Cu bimetallic catalysts’ peroxidation activation have rarely been explored. In this study, Fe/Cu bimetallic materials were fabricated to catalyze different oxidizing agents, including peroxymonosulfate (PMS), peroxydisulfate (PDS), peroxyacetic acid (PAA), and hydrogen peroxide (H2O2), for the degradation of sulfamethoxazole (SMX). The Fe/Cu/oxidant systems exhibited an excellent degradation efficiency of sulfamethoxazole (SMX). In the Fe/Cu/PMS, Fe/Cu/PDS, and Fe/Cu/PAA systems, the main reactive oxygen species (ROS) responsible for SMX degradation were hydroxyl radical (•OH) and singlet oxygen (1O2), while the main ROS was only •OH in the H2O2 system. The differences in the surface structure of the materials before and after oxidation were examined, revealing the presence of a large amount of flocculent material on the surface of the oxidized PMS material. Anion experiments and actual body experiments also revealed that the PMS system had a strong anti-interference ability. Finally, a comprehensive comparison concluded that the PMS system was the optimal system among the four oxidation systems. Overall, this work revealed that the PMS oxidant has a better catalytic degradation of SMX compared to other oxidizers for Fe/Cu, that PMS generates more ROS, and that the PMS system has a stronger resistance to interference.
Thermal Surface Properties, London Dispersive and Polar Surface Energy of Graphene and Carbon Materials Using Inverse Gas Chromatography at Infinite Dilution
Authors: Hamieh Tayssir
Published: June 2024

The thermal surface properties of graphenes and carbon materials are of crucial importance in the chemistry of materials, chemical engineering, and many industrial processes. Background: The determination of these surface properties is carried out using inverse gas chromatography at infinite dilution, which leads to the retention volume of organic solvents adsorbed on solid surfaces. This experimental and fundamental parameter actually reflects the surface thermodynamic interactions between injected probes and solid substrates. Methods: The London dispersion equation and the Hamieh thermal model are used to quantify the London dispersive and polar surface energy of graphenes and carbon fibers as well their Lewis acid-base constants by introducing the coupling amphoteric constant of materials. Results: The London dispersive and polar acid-base surface energies, the free energy of adsorption, the polar enthalpy and entropy, and the Lewis acid-base constants of graphenes and carbon materials are determined. Conclusions: It is shown that graphene exhibited the highest values of London dispersive surface energy, polar surface energy, and Lewis acid-base constants. The highest characteristics of graphene justify its great potentiality and uses in many industrial applications.