An Italian Multicenter Study on Anti-NXP2 Antibodies: Clinical and Serological Associations
Authors: Fredi Micaela , Cavazzana Ilaria , Ceribelli Angela , Cavagna Lorenzo , Barsotti Simone , Bartoloni Elena , Benucci Maurizio , De Stefano Ludovico , Doria Andrea , Emmi Giacomo , Fabris Martina , Fornaro Marco , Furini Federica , Giudizi Maria Grazia , Govoni Marcello , Ghirardello Anna , Iaccarino Luca , Iannone Fiorenzo , Infantino Maria , Isailovic Natasa , Lazzaroni Maria Grazia , Manfredi Mariangela , Mathieu Alessandro , Marasco Emiliano , Migliorini Paola , Montecucco Carlomaurizio , Palterer Boaz , Parronchi Paola , Piga Matteo , Pratesi Federico , Riccieri Valeria , Selmi Carlo , Tampoia Marilina , Tripoli Alessandra , Zanframundo Giovanni , Radice Antonella , Gerli Roberto , Franceschini Franco ,   FIRMA (Forum Italiano per la Ricerca nelle Malattie Autoimmuni)
Published: January 2022

The identification of anti-NXP2 antibodies is considered a serological marker of dermatomyositis (DM), with calcinosis, severe myositis and, in some reports, with cancer. Historically, these associations with anti-NXP2 antibodies have been detected by immunoprecipitation (IP), but in the last few years commercial immunoblotting assays have been released. The aim of this collaborative project was to analyse the clinical features associated to anti-NXP2 antibodies, both with commercial line blot (LB) and IP. Myositis-specific and myositis-associated autoantibodies were detected in single centres by commercial line blot (LB); available sera were evaluated in a single centre by protein and RNA immunoprecipitation (IP), and IP-Western blot. Sixty patients anti-NXP2+ (NXP2+) positive by LB were compared with 211 patients anti-NXP2 negative with idiopathic inflammatory myositis (IIM). NXP2+ showed a younger age at IIM onset (p = 0.0014), more frequent diagnosis of dermatomyositis (p = 0.026) and inclusion-body myositis (p = 0.009), and lower rate of anti-synthetase syndrome (p < 0.0001). As for clinical features, NXP2+ more frequently develop specific skin manifestations and less frequently features related with overlap myositis and anti-synthetase syndrome. IP confirmed NXP2 positivity in 31 of 52 available sera (62%). Most clinical associations were confirmed comparing NXP2 LB+/IP+ versus NXP2-negative myositis, with the following exceptions: inclusion-body myositis diagnosis was not detected, whilst dysphagia and myositis were found more frequently in NXP2 LB+/IP+ patients. The 21 LB+ /IP-myositis patients did not show differences in clinical features when compared with the NXP2-myositis patients and more frequently displayed multiple positivity at LB. Risk of developing cancer-associated myositis was similar between NXP2-positive and NXP2-negative myositis patients, either when detected by LB or IP. Protein-IP confirmed NXP2 antibodies in nearly 60% of sera positive for the same specificity with commercial assay. Double-positive cases rarely occurred in myositis patients with a clinical diagnosis other than dermatomyositis. Patients only positive by LB (LB+/IP−) did not display clinical features typical of NXP2. NXP2 positivity by LB should be confirmed by other methods in order to correctly diagnose and characterize patients affected by idiopathic inflammatory myositis.