bluuebunny/crossref_metadata_2025 huggingface dataset:
Interspecific differences in ecological stoichiometric characteristics of invertebrates and their influencing factors from the Beibu Gulf, China
Authors: Wang Caiguang , Huang Liangliang , Kang Bin , Zhu Liang , Liu Hao , Zhao Shuwen , Cheng Yanan , Shahab Asfandyar , Yan Yunrong
Published: July 2024

Invertebrates can store carbon (C), nitrogen (N), phosphorus (P), and other elements in their body tissues at theoretically homeostatic rates, thus playing an important role in the biogeochemical cycle of aquatic ecosystems. To sustain homeostasis, consumers must either balance their resource supply or adjust their stoichiometric features in response to environmental changes. However, there is limited understanding regarding potential differences in the ecological stoichiometric characteristics of marine invertebrates. To explore the ecological stoichiometric characteristics of marine invertebrates, the C, N, P, calcium (Ca), δ13C, and δ15N contents of 18 invertebrate species were analyzed from the Beibu Gulf. The results revealed that the ranges of elemental variations (C, N, and P) in invertebrates were 25.17%-47.34%, 6.14%-14.13%, and 0.26%-1.31%, respectively. The content of P in invertebrates exhibited the most significant variation, leading to alterations in C:P and N:P ratios. A significant negative correlation was observed between P content and body weight in invertebrates (p < 0.01). Furthermore, the C and N content of invertebrates were significantly negatively correlated with δ13C (p < 0.01), suggesting that the variations in C content and N content in invertebrates are influenced by different food sources, while P content varies according to body size. Our results also indicated significant interspecific differences in the ecological stoichiometry of invertebrates from the Beibu Gulf (p < 0.05). Invertebrate growth may be inhibited by P, and they do not maintain strict homeostasis, with stable homeostasis observed in higher trophic levels.
Case report: Suspected organizing pneumonia secondary to severe respiratory syncytial virus pneumonia in an elderly patient
Authors: Feng Min , Zhang Jie , Li Xiangrui , Wang Shuai , Li Yanxia , Dong Chang
Published: July 2024

Respiratory syncytial virus (RSV) usually causes acute respiratory tract infection in infants. In recent years, it has gradually become an important pathogen of lower respiratory tract infection in elderly people with an underlying disease. However, at present, the treatment of severe RSV pneumonia in adults is unclear, and organizing pneumonia (OP) after severe RSV infection has rarely been reported. We reported a 76-year-old man with multiple chronic heart and lung diseases who presented with fever, cough and progressive dyspnea. Finally, severe RSV pneumonia was diagnosed after his nasopharyngeal swabs and bronchoalveolar lavage metagenomic next-generation sequencing tests were positive for RSV. After combined treatment with oral ribavirin, intravenous immunoglobulin and corticosteroids, the patient’s condition largely resolved, and he was discharged. However, when the corticosteroids were gradually tapered, the disease relapsed twice, and the patient experienced fever and aggravated dyspnea. Despite the lack of pathological evidence, we highly suspected organizing pneumonia secondary to severe RSV pneumonia based on the typical imaging manifestations and the clinical characteristics of a good response to corticosteroids. Finally, this patient was successfully treated with a course of corticosteroids and followed up for 14 months in total.
Fingerprint profiling for quality evaluation and the related biological activity analysis of polysaccharides from Liuweizhiji Gegen-Sangshen beverage
Authors: Wei Shulin , Li Mingxing , Zhao Long , Wang Tiangang , Wu Ke , Yang Jiayue , Tang Mingyun , Zhao Yueshui , Shen Jing , Du Fukuan , Chen Yu , Deng Shuai , Xiao Zhangang , Wei Mei , Li Zhi , Wu Xu
Published: July 2024

Introduction

Liuweizhiji Gegen-Sangshen beverage (LGS) is popular in China, which has been used for alleviating alcohol-mediated discomfort and preventing alcoholic liver disease (ALD). This beverage is consisted of six herbal components that are known as functional foods and fruits. LGS is rich in polysaccharides, however, the activity and quality evaluation of LGS-derived polysaccharides remain unexplored. The purpose of this study is thus to establish a comprehensive quality control methodology for the assessment of LGS polysaccharides (LGSP) and to further explore the anti-oxidant, anti-inflammatory as well as prebiotic effect of LGSP.

Methods

LGSP was extracted, followed by analysis of molecular weight distribution, monosaccharide content and structural characterization via integrating the application of high-performance size exclusion chromatography (HPSEC), 1-phenyl-3-methyl-5-pyrazolone-HPLC (PMP-HPLC), fourier transform infrared spectroscopy (FT-IR) as well as nuclear magnetic resonance spectroscopy (NMR) techniques. The anti-oxidation activity of LGSP was determined by DPPH, ABTS, hydroxyl radical scavenging capacity and total antioxidant capacity. The anti-inflammation of LGSP were assessed on the RAW 264.7 cells. The effect of LGSP on growth of Lactobacillus, Bifidobacterium bifidum and Bifidobacterium adolescentis was evaluated.

Results

The results demonstrated that LGSP had two molecular weight distribution peaks, with the average molecular weights of (6.569 ± 0.12) × 104 Da and (4.641 ± 0.30) × 104 Da. LGSP was composed of 8 monosaccharides, with galacturonic acid, glucose rhamnose and galactose representing the highest molar ratios. Homogalacturonic acid (HG) type and rhamnosegalacturonic acid glycans I (RG-I) type and α-1,4-glucan were present in LGSP. LGSP concentration in LGS was 17.94 ± 0.28 mg/mL. Furthermore, fingerprint analysis combined with composition quantification of 10 batches of LGSP demonstrated that there was a high similarity among batches. Notably, LGSP exhibited anti-oxidant effect and inhibited expressions of pro-inflammatory factors (TNF-α and IL-6) in LPS-stimulated RAW 264.7 cells. In addition, LGSP remarkably promoted the proliferation of probiotics Lactobacillus, Bifidobacterium bifidum and Bifidobacterium adolescentis, showing good prebiotic activity.

Discussion

The results of present study would be of help to gain the understanding of structure–activity relationship of LGSP, provide a reference for quality evaluation of bioactive LGSP, and facilitate development of unique health and functional products in the future.
Challenges in the treatment of BRAF K601E-mutated lung carcinoma: a case report of rapid response and resistance to dabrafenib and trametinib
Authors: Nishiyama Akihiro , Sato Shigeki , Sakaguchi Hiroyuki , Kotani Hiroshi , Yamashita Kaname , Ohtsubo Koushiro , Nanjo Shigeki , Yano Seiji , Mizuguchi Keishi , Ikeda Hiroko , Takeuchi Shinji
Published: July 2024

We report a case of limited effectiveness of dabrafenib and trametinib in a 59-year-old man with poorly differentiated lung carcinoma and a rare BRAF K601E mutation. The patient, unresponsive to chemotherapy and immunotherapy, received these targeted agents as second-line treatment. Despite a notable initial response, tumor regression lasted only 52 days. A subsequent liquid biopsy revealed additional alterations (BRAF amplification, KIT amplification, TP53 S241F), indicating a complex resistance mechanism. This case underscores the challenges in treating BRAF K601E-mutant lung carcinoma, emphasizing the need for advanced molecular diagnostics, personalized approaches, and further research into more effective therapies for unique genetic profiles.
Camrelizumab combined with anlotinib as second-line therapy for metastatic or recurrent small cell lung cancer: a retrospective cohort study
Authors: Shen Shujing , Li Xingya , Guo Sanxing , Xu Liang , Yan Ningning
Published: July 2024

Introduction

This retrospective study evaluates the efficacy of camrelizumab combined with anlotinib versus chemotherapy in patients with extensive-stage small-cell lung cancer (ES-SCLC) undergoing second-line treatment.

Methods

Data were sourced from medical records at a Chinese medical facility, involving 34 patients diagnosed with ES-SCLC after failing first-line treatment. Patients were divided into two groups: one received camrelizumab (200 mg every 3 weeks) with anlotinib (12 mg daily for 14 days followed by a 7-day rest), while the other group received physician-chosen chemotherapy administered every 3 weeks. The primary endpoint was progression-free survival (PFS), with secondary endpoints including overall survival (OS), objective response rate (ORR), and disease control rate (DCR).

Results

The combination therapy group showed a significant improvement in PFS compared to the chemotherapy group (median PFS: 7 months vs. 3 months; hazard ratio (HR): 0.34; 95% confidence interval (CI): 0.15-0.77; p<0.001). However, there was no statistically significant difference in OS between the groups (16.3 months vs. 17.3 months; p=0.82). The ORR was 52.9% in the combination therapy group versus 23.5% in the chemotherapy group (p=0.08), and the DCR was 82.4% compared to 58.8% (p=0.26). Grade 3 or higher adverse events were observed in 17.6% of the combination therapy group and 29.4% of the chemotherapy group.

Conclusions

The findings suggest that the combination of camrelizumab and anlotinib offers a superior anti-tumor response with a manageable safety profile in a second-line setting for ES-SCLC patients. This combination regimen may be a viable option for second-line ES-SCLC treatment.
Recent progress in Surface-Enhanced Raman Spectroscopy detection of biomarkers in liquid biopsy for breast cancer
Authors: Liu Xiaobei , Jia Yining , Zheng Chao
Published: July 2024

Breast cancer is the most commonly diagnosed cancer in women globally and a leading cause of cancer-related mortality. However, current detection methods, such as X-rays, ultrasound, CT scans, MRI, and mammography, have their limitations. Recently, with the advancements in precision medicine and technologies like artificial intelligence, liquid biopsy, specifically utilizing Surface-Enhanced Raman Spectroscopy (SERS), has emerged as a promising approach to detect breast cancer. Liquid biopsy, as a minimally invasive technique, can provide a temporal reflection of breast cancer occurrence and progression, along with a spatial representation of overall tumor information. SERS has been extensively employed for biomarker detection, owing to its numerous advantages such as high sensitivity, minimal sample requirements, strong multi-detection ability, and controllable background interference. This paper presents a comprehensive review of the latest research on the application of SERS in the detection of breast cancer biomarkers, including exosomes, circulating tumor cells (CTCs), miRNA, proteins and others. The aim of this review is to provide valuable insights into the potential of SERS technology for early breast cancer diagnosis.
Neutrophilia in the bronchoalveolar lavage fluid increases coughing during flexible fiberoptic bronchoscopy in a pediatric cohort
Authors: Danino Laura , Stehling Florian , Eckerland Maximilian , Orhan Eser , Tschiedel Eva
Published: July 2024

Objective

This study is an addition to the already published prospective randomized double-blinded trial by Tschiedel et al. that compared two different sedation regimes in fiberoptic flexible bronchoscopy in pediatric subjects. The objective of the presented study is to analyze the correlation between the neutrophil percentage of the bronchoalveolar lavage fluid (BALF) and coughing episodes during bronchoscopy.

Methods

Fifty subjects, aged 1–17 years, received flexible fiberoptic bronchoscopy under deep sedation. The BALF of 39 subjects was analyzed with reference to cytology and microbiology.

Results

The percentage of neutrophils from the total cell count ranged from 0% to 95.3% (median 2.7). Nineteen patients (49%) had a percentage of ≥3.0%. Pearson's correlation showed a high correlation (r = 0.529, p = 0.001) between the coughing episodes per minute and the neutrophil percentage in the BALF. Analysis of variance showed a significant difference in neutrophil percentage between the indication groups (p = 0.013). The t-test (p = 0.019) showed a significant difference between the neutrophil percentage for patients with a probable airway infection under immunosuppression (median 2.9) and patients with cystic fibrosis (median 49.6). The linear regression analysis showed a significantly stronger impact of the neutrophil percentage on coughing frequency than the sedation regime (βneutrophils = 0.526 with p = 0.001 vs. βsedation = 0.165 with p = 0.251).

Conclusion

When bronchoscopy is to be performed on a pediatric patient with suspected bacterial or viral infection, and therefore neutrophilic airway inflammation, coughing is to be expected.
Cyclophilin inhibition as a strategy for the treatment of human disease
Authors: Stauffer Winston T. , Goodman Asha Z. , Gallay Philippe A.
Published: July 2024

Cyclophilins (Cyps), characterized as peptidyl-prolyl cis-trans isomerases (PPIases), are highly conserved and ubiquitous, playing a crucial role in protein folding and cellular signaling. This review summarizes the biochemical pathways mediated by Cyps, including their involvement in pathological states such as viral replication, inflammation, and cancer progression, to underscore the therapeutic potential of Cyp inhibition. The exploration of Cyp inhibitors (CypI) in this review, particularly non-immunosuppressive cyclosporine A (CsA) derivatives, highlights their significance as therapeutic agents. The structural and functional nuances of CsA derivatives are examined, including their efficacy, mechanism of action, and the balance between therapeutic benefits and off-target effects. The landscape of CypI is evaluated to emphasize the clinical need for targeted approaches to exploit the complex biology of Cyps and to propose future directions for research that may enhance the utility of non-immunosuppressive CsA derivatives in treating diseases where Cyps play a key pathological role.
FGF21-dependent alleviation of cholestasis-induced liver fibrosis by sodium butyrate
Authors: Yang Jing , Chen Lei , Zhao Shan-Shan , Du Chuang , Fan Yi-Zhe , Liu Hui-Xin , Li Yongchun , Li Yong-Zhi
Published: July 2024

Background

The beneficial effects of fibroblast growth factor 21 (FGF21) and sodium butyrate (NaB) on protection against cholestasis-induced liver fibrosis are not well known. This study aimed to explore the effects of FGF21 and NaB on bile duct ligation (BDL)-induced liver fibrosis.

Methods

Wild-type (WT) and FGF21 knockout (KO) mice received BDL surgery for 14 days. Liver fibrosis was assessed by Masson’s staining for fibrosis marker expressions at the mRNA or protein levels. Adenovirus-mediated FGF21 overexpression in the WT mice was assessed against BDL damage. BDL surgeries were performed in WT and FGF21 KO mice that were administered either phosphate-buffered saline or NaB. The effects of NaB on the energy metabolism and gut microbiota were assessed using stable metabolism detection and 16S rRNA gene sequencing.

Results

BDL-induced liver fibrosis in the WT mice was accompanied by high induction of FGF21. Compared to the WT mice, the FGF21 KO mice showed more severe liver fibrosis induced by BDL. FGF21 overexpression protected against BDL-induced liver fibrosis, as proved by the decreasing α-SMA at both the mRNA and protein levels. NaB administration enhanced the glucose and energy metabolisms as well as remodeled the gut microbiota. NaB alleviated BDL-induced liver fibrosis in the WT mice but aggravated the same in FGF21 KO mice.

Conclusion

FGF21 plays a key role in alleviating cholestasis-induced liver damage and fibrosis. NaB has beneficial effects on cholestasis in an FGF21-dependent manner. NaB administration can thus be a novel nutritional therapy for treating cholestasis via boosting FGF21 signaling and regulating the gut microbiota.
Extracting fetal heart signals from Doppler using semi-supervised convolutional neural networks
Authors: Hirono Yuta , Kai Chiharu , Yoshida Akifumi , Sato Ikumi , Kodama Naoki , Uchida Fumikage , Kasai Satoshi
Published: July 2024

Cardiotocography (CTG) measurements are critical for assessing fetal wellbeing during monitoring, and accurate assessment requires well-traceable CTG signals. The current FHR calculation algorithm, based on autocorrelation to Doppler ultrasound (DUS) signals, often results in periods of loss owing to its inability to differentiate signals. We hypothesized that classifying DUS signals by type could be a solution and proposed that an artificial intelligence (AI)-based approach could be used for classification. However, limited studies have incorporated the use of AI for DUS signals because of the limited data availability. Therefore, this study focused on evaluating the effectiveness of semi-supervised learning in enhancing classification accuracy, even in limited datasets, for DUS signals. Data comprising fetal heartbeat, artifacts, and two other categories were created from non-stress tests and labor DUS signals. With labeled and unlabeled data totaling 9,600 and 48,000 data points, respectively, the semi-supervised learning model consistently outperformed the supervised learning model, achieving an average classification accuracy of 80.9%. The preliminary findings indicate that applying semi-supervised learning to the development of AI models using DUS signals can achieve high generalization accuracy and reduce the effort. This approach may enhance the quality of fetal monitoring.
Dentin matrix protein 1 and HUVEC-ECM scaffold promote the differentiation of human dental pulp stem cells into endothelial lineage: implications in regenerative medicine
Authors: Ganapathy Amudha , Narayanan Karthikeyan , Chen Yinghua , Villani Cassandra , George Anne
Published: July 2024

Reprograming of the dental pulp somatic cells to endothelial cells is an attractive strategy for generation of new blood vessels. For tissue regeneration, vascularization of engineered constructs is crucial to improve repair mechanisms. In this study, we show that dentin matrix protein 1 (DMP1) and HUVEC-ECM scaffold enhances the differentiation potential of dental pulp stem cells (DPSCs) to an endothelial phenotype. Our results show that the differentiated DPSCs expressed endothelial markers CD31 and VE-Cadherin (CD144) at 7 and 14 days. Expression of CD31 and VE-Cadherin (CD144) were also confirmed by immunofluorescence. Furthermore, flow cytometry analysis revealed a steady increase in CD31 and VE-Cadherin (CD144) positive cells with DMP1 treatment when compared with control. In addition, integrins specific for endothelial cells were highly expressed during the differentiation process. The endothelial cell signature of differentiated DPSCs were additionally characterized for key endothelial cell markers using gene expression by RT-PCR, Western blotting, immunostaining, and RNA-seq analysis. Furthermore, the angiogenic phenotype was confirmed by tubule and capillary sprout formation. Overall, stimulation of DPSCs by DMP1 and use of HUVEC-ECM scaffold promoted their differentiation into phenotypically, transcriptionally, and functionally differentiated bonafide endothelial cells. This study is novel, physiologically relevant and different from conventional strategies.
Factor structure of the Chinese version of Emotion Regulation Goals Scale
Authors: Chen Shengdong , Chi Cheng , Luo Li , Zhu Weiwei , Chen Yi , Wang Tao , Yuan Jiajin
Published: July 2024

Recent studies in Western cultures suggested emotion regulation goals have important implications for mental health. This study aimed to test the factor structure of Emotion Regulation Goals Scale (ERGS) in a Chinese cultural context. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were first used to examine the factor structure of the ERGS, and then reliability and validity tests were conducted to examine the psychometric properties of the ERGS. Results showed that the original five-factor model demonstrated fit during both EFA and CFA, and was thus adopted for further psychometric analyses. Most of the five factors were significantly associated with emotion regulation tendencies and negative emotional outcomes (e.g., depression), except for the non-significant associations between pro-hedonic goals and expressive suppression, and pro-social and impression management goals with depression. The ERGS also showed good internal consistency and split-half reliability. However, the test-retest reliabilities varied substantially across the five factors. The pro-hedonic goal had a higher test-retest reliability, whereas the contra-hedonic, performance, pro-social, and impression management goals showed lower values, especially the latter two. In brief, the ERGS showed a promising five-factor structure in assessing emotion regulation goals in Chinese cultural context.
Cariprazine in the management of emotionally unstable personality disorder in female patients: a case series
Authors: Pappa Sofia , Caldwell-Dunn Ellice , Kalniunas Arturas , Kamal Manzar
Published: July 2024

Background

Emotionally unstable personality disorder (EUPD) is debilitating psychiatric disorder, particularly common in female and forensic populations. However, appropriate pharmacological treatment to effectively manage symptoms of EUPD remains an unmet clinical need. Dopamine receptor partial agonists (DRPAs), such as aripiprazole, have a favourable tolerability profile and have demonstrated some benefits in targeting symptoms of emotional dysregulation, although, evidence regarding the effects of novel D2/D3 DRPA cariprazine in EUPD patients has been limited.

Objectives

To evaluate the efficacy and tolerability of cariprazine for EUPD in a case series of female forensic inpatients where the diagnosis is more prevalent.

Methods

Demographic and clinical information of the patients were collected from patient electronic records during their admission in a specialized NHS forensic service. Treatment response was measured using the Positive and Negative Syndrome Scale (PANSS) at baseline, 3 and 6 months and Global Clinical Impression Scale (CGI-scores) at baseline and 6 months. Tolerability and BMI, ECG QTc interval and prolactin levels were recorded prior to initiation and at 6 months.

Results

Eight female patients with EUPD (mean age 29.8 years, SD 5.3) were treated with cariprazine (range 3–6mg). Total CGI-scores modestly improved from 5.6 baseline to 5.0 at 6 months. There was a reduction in mean total PANSS scores from baseline to 6 months (92.5, SD 8.1 to 72.4, SD 15.8), general psychopathology (56.1 SD 6.7 to 42.5, SD9.7), positive (21.9 SD 4.6 to 17.1, SD4.8) and negative PANSS scores (14.5 SD 6.3 to 12.8, SD4.6), corresponding to a 21%, 23%, 20% and 3% mean score reduction, respectively. Cariprazine demonstrated a favourable metabolic and hormonal side effect profile with no treatment discontinuation at 6 months follow up.

Conclusion

This is the first case series to evaluate the effectiveness of cariprazine in EUPD. Its efficacy in improving PANSS and CGI-S scores was overall modest and highly variable, reflective of an inherently heterogenous and comorbid patient sample but the benefits on treatment perseverance and tolerability were considerable. Cariprazine may be of particular benefit in EUPD where psychotic symptoms are co-morbid, as an augmentation strategy to clozapine, or where previous antipsychotics have caused metabolic or hormonal side effects.
Exploring the relationship between vaccine hesitancy and mothers’ perspectives on COVID-19 vaccines for children ages 5–11 years during the omicron predominant period 2021–2022: a qualitative study
Authors: Suragh Tiffany A. , Adzrago David , Allicock Marlyn A. , Yeh Paul G. , Cuccaro Paula
Published: July 2024

Background

The United States Food and Drug Administration authorized COVID-19 vaccines for children ages 5–11 years in October 2021 during the Omicron predominant period. Parental vaccine hesitancy was prevalent during this time, resulting in low childhood COVID-19 vaccine uptake. Most studies exploring factors influencing parental vaccine hesitancy have focused on racial and ethnic minorities and lower socioeconomic populations; however, there is little knowledge of the drive drivers of vaccine hesitancy among White parents with higher education and socioeconomic statuses.

Methods

We conducted semi-structured interviews with a sample of 15 White mothers of children ages 5–11 years in Atlanta, GA, between October–December 2021. Thematic analysis was performed using NVivo 12.

Results

Mothers were college-educated, homeowners, and fully vaccinated against COVID-19. Key findings included decreased pediatrician’s recommendations for COVID-19 vaccines, reliance on information from specialized doctors and scientists, distrust in public health authorities, high risk-perception of COVID-19 vaccines, and low risk-perception of COVID-19 disease. Factors related to vaccine acceptance were altruism and practicality.

Conclusion

This study adds to the sparse literature on reasons for vaccine hesitancy among White mothers of children ages 5–11 years with higher educational and socioeconomic status. Improving vaccine uptake among this group is critical for protecting the health of their children and other vulnerable populations. Tailored vaccine messaging and intervention are warranted to address their unique attitudes, beliefs, and behaviors. An enhanced understanding of the factors influencing subpopulations of parents can help vaccine policymakers and healthcare providers improve efforts to reduce vaccine hesitancy, particularly for new vaccines.
Data quality and timeliness analysis for post-vaccination adverse event cases reported through healthcare data exchange to FDA BEST pilot platform
Authors: Deady Matthew , Duncan Ray , Jones Lance D. , Sang Arianna , Goodness Brian , Pandey Abhishek , Cho Sylvia , Forshee Richard A. , Anderson Steven A. , Ezzeldin Hussein
Published: July 2024

Introduction

This study is part of the U.S. Food and Drug Administration (FDA)’s Biologics Effectiveness and Safety (BEST) initiative, which aims to improve the FDA’s postmarket surveillance capabilities by using real-world data (RWD). In the United States, using RWD for postmarket surveillance has been hindered by the inability to exchange clinical data between healthcare providers and public health organizations in an interoperable format. However, the Office of the National Coordinator for Health Information Technology (ONC) has recently enacted regulation requiring all healthcare providers to support seamless access, exchange, and use of electronic health information through the interoperable HL7 Fast Healthcare Interoperability Resources (FHIR) standard. To leverage the recent ONC changes, BEST designed a pilot platform to query and receive the clinical information necessary to analyze suspected AEs. This study assessed the feasibility of using the RWD received through the data exchange of FHIR resources to study post-vaccination AE cases by evaluating the data volume, query response time, and data quality.

Materials and methods

The study used RWD from 283 post-vaccination AE cases, which were received through the platform. We used descriptive statistics to report results and apply 322 data quality tests based on a data quality framework for EHR.

Results

The volume analysis indicated the average clinical resources for a post-vaccination AE case was 983.9 for the median partner. The query response time analysis indicated that cases could be received by the platform at a median of 3 min and 30 s. The quality analysis indicated that most of the data elements and conformance requirements useful for postmarket surveillance were met.

Discussion

This study describes the platform’s data volume, data query response time, and data quality results from the queried postvaccination adverse event cases and identified updates to current standards to close data quality gaps.
The sweet symphony of N-glycans in myeloid malignancies
Authors: Sanmartín-Martínez Javier , Wiersma Valerie R. , Marneth Anna E.
Published: July 2024

Although the involvement of glycan structures in diseases has long been recognized, their detailed and high-throughput investigation has only recently been made possible due to technological advancements. For this reason, glycosylation is a generally understudied phenomenon, however it could provide critical information on the pathobiology of many disorders by virtue of its widespread abundance and critical role in protein function. Here, we focus on myeloid malignancies, conditions for which the survival rates are often poor and curative therapeutic options are generally limited. We review the current literature on (1) N-glycosylation of major hematopoietic growth receptors found mutated in myeloid malignancies, (2) chemoresistance through intracellular glycan-related processes, and (3) mechanisms by which altered N-glycosylation contributes to interactions between myeloid blasts and bone marrow stromal cells leading to niche hijacking. For each topic, we describe the related pathobiology and its (potential) clinical implications. The combination of glycoproteomic and genomic information is expected to result in a deeper molecular understanding of the pathobiology of these diseases, which could subsequently be used for improving prognostication and therapeutic strategies.
An economical self-coalescing microfluidic device with an easily observable readout
Authors: Kamat Lily , Delgado Priscilla , Dissanayaka Anjana , Myers David R.
Published: July 2024

Introduction: Self-coalescing microfluidic devices represent an exciting opportunity for leveraging viscous dominated flow phenomena to reconstitute a series of reagents with a single sample infusion. In a self-coalescing microfluidic device, spatially separated dried reagents are reconstituted using a single infusion. Due to the unique device geometry, the reagents remain spatially separated after infusion. As such, self-coalescing microfluidic devices have the potential to simplify point-of-care testing by simultaneously performing multiple colorimetric tests in one device. The current versions of these devices use standard, more costly microfabrication processes and are too small to be easily read by eye.

Methods: Here, we created a low cost and scaled up version of a self-coalescing microfluidic device by using laser-cut-roll-based silicone tape. In addition to eliminating the need for cleanrooms, our approach simplifies the integration of assay reagents since they can be spotted onto a coverslip and covered with the tape microfluidic device. We empirically optimized our device, finding that flow rate significantly influenced the formation of self-coalescence as well as proper reagent reconstitution.

Results and Discussion: When flow rates were too slow or fast, reagents would streak, leading to inadvertent mixing between different spatial locations. Our studies further revealed that geometry had a stronger influence on device operation in low-flow conditions. Our final optimized device exhibited a 100% success rate, demonstrated through self-coalescence with no reagent streaking at 100 μL/min, which enabled the isotropic diffusion of reagents. Furthermore, the spots are spatially separated and large enough to be visualized by the naked eye and captured by a smartphone camera for downstream analysis. Taken together, our innovative device leverages the advantages of sticker microfluidics and low-cost manufacturing methods to offer standalone functionality. This approach has the potential to significantly impact point-of-care diagnostics, particularly in resource-limited regions. By enabling multiplexed diagnostic assays through our novel technology, we aim to provide accessible and affordable healthcare solutions.
Kinanthropometry and dietary habits of non-professional rugby players
Authors: Holway Francis E. , Campa Francesco , Petri Cristian , Spena Luciano R. , Szydlowski Natalia Y.
Published: July 2024

Introduction

Evaluating the body composition and dietary habits of non-professional athletes can help identify areas for improvement to enhance sports performance. The present study aimed to describe the anthropometric and body composition features, as well as the dietary habits, of non-professional rugby players in Argentina.

Methods

Fifty-seven rugby players from a Group III Club of the Unión de Rugby de Buenos Aires (URBA) were assessed using extensive anthropometric measurements according to the International Society for the Advancement of Kinanthropometry (ISAK) protocol. Reference data from professional rugby players in Group I clubs were used as a control for body composition comparisons. Dietary intake was evaluated using the 24-h recall method, and nutrient analysis was performed with SARA software.

Results

Non-professional rugby players were shorter (Forwards: 175.9 vs. 181.5 cm; Backs: 172.5 vs. 175.7 cm), had higher body fat percentages (Forwards: 16.4 vs. 12.3%; Backs: 11.0 vs. 9.3%), and were less muscular (Forwards: 46.0 vs. 48.8%; Backs: 48.4 vs. 50.2%) compared to professional rugby players. The average dietary intake was 3,363 Kcal, with protein and carbohydrate intakes of 1.4 g kg−1 day−1 and 4.1 g kg−1 day−1, respectively, and 35% of energy intake from fat. Backs reported a higher caloric intake than forwards (3,682 vs. 2,827 Kcal). There was a high prevalence of insufficient intake of calcium (58%), vitamin A (49%), and vitamin C (65%), the latter two corresponding with a low intake of fruits and vegetables (6% of total energy intake). Meal pattern analysis showed that 46% of total energy was ingested at dinner.

Conclusions

The body composition of non-professional rugby players from low-income clubs could be improved to enhance rugby performance, as compared to players in more competitive tiers. Economic constraints might contribute to a sub-optimal nutritional profile, potentially affecting body composition and on-field performance negatively. Recommendations to improve dietary intake should be made considering the budget constraints of these players.
Whole-genome resequencing of native and imported dairy goat identifies genes associated with productivity and immunity
Authors: Zhao Jianqing , Mu Yuanpan , Gong Ping , Liu Baolong , Zhang Fuhong , Zhu Lu , Shi Chenbo , Lv Xuefeng , Luo Jun
Published: July 2024

Understanding the differences in genetic variation between local Chinese dairy goat breeds and imported breeds can help germplasm innovation and molecular breeding. However, the research is limited in this area. In this study, whole-genome resequencing data from 134 individuals of both local and imported dairy goat breeds were analyzed, and their differences in genomic genetic variation, genetic diversity, and population structure were subsequently identified. We also screened candidate genes associated with important traits of dairy goats such as milk production (STK3, GHR, PRELID3B), reproduction (ATP5E), growth and development (CTSZ, GHR), and immune function (CTSZ, NELFCD). Furthermore, we examined allele frequency distributions for the genes of interest and found significant differences between the two populations. This study provides valuable resources for the study of genetic diversity in dairy goats and lays the foundation for the selective breeding of dairy goats in the future.
SCMs: Systematic Conglomerated Models for Audio Cough Signal Classification
Authors: Prabhakar Sunil Kumar , Won Dong-Ok
Published: July 2024

A common and natural physiological response of the human body is cough, which tries to push air and other wastage thoroughly from the airways. Due to environmental factors, allergic responses, pollution or some diseases, cough occurs. A cough can be either dry or wet depending on the amount of mucus produced. A characteristic feature of the cough is the sound, which is a quacking sound mostly. Human cough sounds can be monitored continuously, and so, cough sound classification has attracted a lot of interest in the research community in the last decade. In this research, three systematic conglomerated models (SCMs) are proposed for audio cough signal classification. The first conglomerated technique utilizes the concept of robust models like the Cross-Correlation Function (CCF) and Partial Cross-Correlation Function (PCCF) model, Least Absolute Shrinkage and Selection Operator (LASSO) model, elastic net regularization model with Gabor dictionary analysis and efficient ensemble machine learning techniques, the second technique utilizes the concept of stacked conditional autoencoders (SAEs) and the third technique utilizes the concept of using some efficient feature extraction schemes like Tunable Q Wavelet Transform (TQWT), sparse TQWT, Maximal Information Coefficient (MIC), Distance Correlation Coefficient (DCC) and some feature selection techniques like the Binary Tunicate Swarm Algorithm (BTSA), aggregation functions (AFs), factor analysis (FA), explanatory factor analysis (EFA) classified with machine learning classifiers, kernel extreme learning machine (KELM), arc-cosine ELM, Rat Swarm Optimization (RSO)-based KELM, etc. The techniques are utilized on publicly available datasets, and the results show that the highest classification accuracy of 98.99% was obtained when sparse TQWT with AF was implemented with an arc-cosine ELM classifier.
An Experimental Investigation of Low-Frequency Active Excitation in Scramjet Combustor Using a Micro-Pulse Detonation Engine
Authors: Lee Keon-Hyeong , Kim Min-Su , Choi Jeong-Yeol , Yu Kenneth H.
Published: July 2024

A micro-pulse detonation engine (μPDE) was designed and installed to a direct-connect scramjet combustor of Pusan National University (PNU-DCSC). The active excitation on the scramjet combustor was experimentally studied using the μPDE operating at frequencies of 10–20 Hz. A vitiation air heater (VAH) was used to supply high-enthalpy vitiated air to the isolator and the scramjet combustor at a Mach number of 2.0, with a total temperature of 1600 K and a total pressure of 1.68 MPa. The exit of μPDE was located at the center of the cavity of the scramjet combustor. Active excitation was performed at equivalence ratios of 0.111 and 0.163, and characteristics were analyzed through Schlieren recording and bottom wall pressure measurement. As a result, when the detonation emitted from the μPDE entered the scramjet combustor, it instantly formed a shock train and moved forward within the scramjet combustor. The flame instantaneously changed from the cavity shear layer flame to the cavity flame. Through bottom wall pressure measurement, it was also observed that active excitation resulted in a significant pressure increase near the cavity compared to when active excitation was not performed. This revealed combustion characteristics, indicating improved combustion efficiency from the pressure increase in the scramjet combustor.
Balancing Greenhouse Gas Emissions and Yield through Rotational Tillage in the Cold Rice-Growing Region
Authors: Dong Wenjun , Tang Ao , Zhang Jun , Liu Youhong , Meng Ying , Zhang Xijuan , Wang Lizhi , Yang Zhongliang
Published: July 2024

Tillage practices are of critical importance in maintaining soil quality on cropland and for food production, with rice cultivation representing a significant portion of the world’s food production and greenhouse gas (GHG) emissions. While numerous studies have examined the effects of reduced and no-tillage on soil GHG emissions and rice yields, the impact of adopting a rotational approach to tillage practices on the rice cultivation cycle remains uncertain. In this study, we conducted a four-year (2017–2020) field experiment in a single rice-growing area in Northeast China with the aim of investigating the effects of different tillage practices on GHG emissions from paddy fields and rice yields under full straw return conditions. We set up three experimental treatments: rotary tillage, plowing, and rotational tillage (i.e., a combination of one year of plowing and one year of rotary tillage). The results showed that averaged across all treatments, average methane (CH4, 302.6 ± 51.1 kg ha−1) and nitrous oxide (N2O, 0.86 ± 0.361 kg ha−1) emissions and rice yield (9.0 ± 0.9 t ha−1) did not exhibit significant inter-annual variability during the entire experimental period and were comparable to the average for the region. The ranking of GHG emissions during the rice-growing season was as follows: rotary tillage > plowing > rotational tillage. Across the experimental period, CH4 and N2O emissions were 9.1% and 8.5% lower in the plowing treatment and 21.2% and 13.1% lower in the rotational tillage treatment compared to the rotary tillage treatment. During the experimental period, there was no significant effect of tillage treatments on rice yield. This reduction in emissions may be attributed to changes in soil penetration resistance. In the rotational and plowing treatments, soil penetration resistance was in a range more adapted to rice growth and GHG emissions reduction compared to the rotary tillage treatment. The yield-scale GHG emission intensity was reduced by 12.7% and 26.1% in the plowing and rotational tillage treatments, respectively, in comparison to the rotary tillage treatment. This suggests that rotational tillage is a management practice that can achieve greenhouse gas emission reductions in paddy fields and stabilize or possibly increase rice yields. Consequently, the results demonstrated that a rotational alternation of multiple tillage practices is a synergistic strategy for achieving low carbon and high yield in rice in the cold rice-growing region of Northeast China.
Exploring the Evolution of Postharvest Quality and Composition in Novel Mini Plum Tomatoes with Different Fruit Pigmentations
Authors: Cannata Claudio , Mauro Rosario Paolo , Rutigliano Chiara Alessandra Carmen , Basile Federico , Muratore Giuseppe , Restuccia Cristina , Sabatino Leo , Leonardi Cherubino
Published: July 2024

This study evaluated the postharvest evolution in terms of quality and functional traits of recently introduced mini plum tomato cultivars, differing for fruit pigmentation. The cultivars Santy Naranja (orange fruit) and Melange (dark red, green-striped fruit), along with the well-established ‘Angelle’ (red fruit), were monitored during refrigerated storage (10 °C) for 0, 7, and 14 days (hereafter S0, S7, and S14, respectively) inside closed polylactic acid trays. ‘Melange’ displayed the highest fruit fresh weight loss and total soluble solids (TSS) (9.28 °Bx), along with the lowest firmness (4.69 N), whereas ‘Santy Naranja’ showed the strongest color deviation during the storage period. Titratable acidity (TA) was relatively stable in ‘Melange’, while ‘Angelle’ experienced the highest decrease between S7 and S14, resulting in stronger TSS/TA fluctuations. Regarding the functional traits, ‘Melange’ had the highest total polyphenols content (TPC), whereas ‘Angelle’ excelled in total carotenoid content (TCC), ascorbic acid content (AsA), and fruits’ antioxidant activity, as revealed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. With reference to storage time, the O2 concentration in the headspace, TPC, AsA, and DPPH decreased between S0 and S14, whereas CO2 concentration, TCC, and FRAP increased, but these variations were mostly cultivar-dependent. This study provides insights into the postharvest behavior of novel mini plum tomato typologies and their ability to maintain quality over time, providing information for developing strategies to preserve their sensory and nutraceutical characteristics during refrigerated storage.
Novel Antibiotics for Gram-Negative Nosocomial Pneumonia
Authors: Almyroudi Maria Panagiota , Chang Aina , Andrianopoulos Ioannis , Papathanakos Georgios , Mehta Reena , Paramythiotou Elizabeth , Koulenti Despoina
Published: July 2024

Nosocomial pneumonia, including hospital-acquired pneumonia and ventilator-associated pneumonia, is the leading cause of death related to hospital-acquired infections among critically ill patients. A growing proportion of these cases are attributed to multi-drug-resistant (MDR-) Gram-negative bacteria (GNB). MDR-GNB pneumonia often leads to delayed appropriate treatment, prolonged hospital stays, and increased morbidity and mortality. This issue is compounded by the increased toxicity profiles of the conventional antibiotics required to treat MDR-GNB infections. In recent years, several novel antibiotics have been licensed for the treatment of GNB nosocomial pneumonia. These novel antibiotics are promising therapeutic options for treatment of nosocomial pneumonia by MDR pathogens with certain mechanisms of resistance. Still, antibiotic resistance remains an evolving global crisis, and resistance to novel antibiotics has started emerging, making their judicious use crucial to prolong their shelf-life. This article presents an up-to-date review of these novel antibiotics and their current role in the antimicrobial armamentarium. We critically present data for the pharmacokinetics/pharmacodynamics, the in vitro spectrum of antimicrobial activity and resistance, and in vivo data for their clinical and microbiological efficacy in trials. Where possible, available data are summarized specifically in patients with nosocomial pneumonia, as this cohort may exhibit ‘critical illness’ physiology that affects drug efficacy.
Differences in the Dwell Time of Peripherally Inserted Central Catheters between Patients with Catheter Colonization and Those Developing Central Line-Associated Bloodstream Infection: A Single Centre Retrospective Cohort Study
Authors: Pitiriga Vassiliki C. , Campos Elsa , Bakalis John , Saroglou George , Tsakris Athanasios
Published: July 2024

Substantial knowledge gaps exist concerning the varying durations of peripherally inserted central catheter (PICC) placements that lead to either central line-associated bloodstream infection (CLABSI) or catheter colonization. We aimed to compare PICCs dwell time between patients who developed CLABSIs due to multidrug-resistant microorganisms (MDROs) and patients with catheter colonization by MDROs. Data from 86 patients admitted consecutively to a tertiary-care hospital from 2017 to 2020 were retrospectively analyzed. The mean dwell time was 25.73 ± 16.19 days in the PICC-CLABSI group and 16.36 ± 10.28 days in the PICC-colonization group (p = 0.002). The mean dwell time was 17.38 ± 9.5 days in the PICC-MDRO group and 22.48 ± 15.64 days in the PICC-non-MDRO group (p = 0.005). Within the PICC-CLABSI group, the mean dwell time for CLABSIs caused by MDROs was 21.50 ± 12.31 days, compared to 27.73 ± 16.98 days for CLABSIs caused by non-MDROs (p = 0.417). Within the PICC-colonization group, the mean dwell time was 15.55 ± 7.73 days in PICCs colonized by MDROs and 16.92 ± 11.85 days in PICCs colonized by non-MDROs (p = 0.124). The findings of the present study suggest that CLABSIs caused by MDROs in PICCs are associated with a shorter mean catheter dwell time compared to those caused by non-MDROs, underscoring the importance of considering infections by MDROs when evaluating PICC dwell times.