bluuebunny/crossref_metadata_2025 huggingface dataset:
Bisphenol A Induces Histopathological, Hematobiochemical Alterations, Oxidative Stress, and Genotoxicity in Common Carp (Cyprinus carpio L.)
Authors: Afzal Gulnaz , Ahmad Hafiz Ishfaq , Hussain Riaz , Jamal Adil , Kiran Shumaila , Hussain Tarique , Saeed Saba , Nisa Mehr un
Published: January 2022

Bisphenol A (BPA) is one of the environmental endocrine disrupting toxicants and is widely used in the industry involving plastics, polycarbonate, and epoxy resins. This study was designed to investigate the toxicological effects of BPA on hematology, serum biochemistry, and histopathology of different organs of common carp (Cyprinus carpio). A total of 60 fish were procured and haphazardly divided into four groups. Each experimental group contained 15 fish. The fish retained in group A was kept as the untreated control group. Three levels of BPA 3.0, 4.5, and 6 mg/L were given to groups B, C, and D for 30 days. Result indicated significant reduction in hemoglobin (Hb), lymphocytes, packed cell volume (PCV), red blood cells (RBC), and monocytes in a dose-dependent manner as compared to the control group. However, significantly higher values of leucocytes and neutrophils were observed in the treated groups (

P
<
0.05

). Results on serum biochemistry revealed that the quantity of glucose, cholesterol, triglycerides, urea, and creatinine levels was significantly high (

P
<
0.05

). Our study results showed significantly (

P
<
0.05

) increase level of oxidative stress parameters like reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) and lower values of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) in treated groups (4.5 mg/L and 6 mg/L)) in the brain, liver, gills, and kidneys. Our study depicted significant changes in erythrocytes (pear shaped erythrocytes, leptocytes, microcytes, spherocytes, erythrocytes with broken, lobed, micronucleus, blabbed, vacuolated nucleus, and nuclear remnants) among treated groups (4.5 mg/L and 6 mg/L). Comet assay showed increased genotoxicity in different tissues including the brain, liver, gills, and kidneys in the treated fish group. Based on the results of our experiment, it can be concluded that the BPA exposure to aquatic environment is responsible for deterioration of fish health, performance leading to dysfunction of multiple vital organs.
Artificial Intelligence Algorithm-Based Computed Tomography Image of Both Kidneys in Diagnosis of Renal Dysplasia
Authors: Liu Yonghui , Tang Siai
Published: January 2022

The objective of this study was to explore the accuracy of low-dosage computed tomography (CT) images based on the expectation maximization algorithm denoising algorithm (EM algorithm) in the detection and diagnosis of renal dysplasia, so as to provide reasonable research basis for accuracy improvement of clinical diagnosis of renal dysplasia. 120 patients with renal dysplasia in hospital were randomly selected as the research objects, and they were divided into two groups by random number method, with 60 patients in each group. The low-dosage CT images of patients in the control group were not processed (nonalgorithm group), and the low-dosage CT images of patients in the observation group were denoised using the EM algorithm (algorithm group). In addition, it was compared with the results of the comprehensive diagnosis (gold standard) to analyze the accuracy of the diagnosis of the two groups of patients and the consistency with the results of the pathological diagnosis. The results were compared with those of the comprehensive diagnosis (gold standard) to analyze the accuracy of the diagnosis of the two groups of patients. The results showed that the peak signal-to-noise ratio (PSNR) (15.9 dB) of the EM algorithm was higher than the regularized adaptive matching pursuit (RAMP) algorithm (1.69 dB) and the mean filter (4.3 dB) (

P
<
0.05

). The time consumption of EM algorithm (21 s) was shorter than that of PWLS algorithm (34 s) and MS-PWLS algorithm (39 s) (

P
<
0.05

). The diagnosis accuracy of dysplasia of single kidney, absence of single kidney, horseshoe kidney, and duplex kidney was obviously higher in the algorithm group than the control group (

P
<
0.05

), which were 66.67% vs. 90%, 60% vs. 88.89%, 71.42% vs. 100%, and 60% vs. 88.89%, respectively. The incidence of hypertension in patients with autosomal dominant polycystic kidney disease (ADPKD) (56.77%) was much higher than that of the other diseases (

P
<
0.05

). After denoising by the EM algorithm, low-dosage CT image could improve the diagnostic accuracy of several types of renal dysplasia except ADPKD, showing certain clinical application value. In addition, ADPKD was easy to cause hypertension.
Geothermal Resource Exploration in Magmatic Rock Areas Using a Comprehensive Geophysical Method
Authors: Zhu Jie , Jin Sheng , Yang Yang , Zhang Tianyu
Published: January 2022

Geothermal resources have significant development and usage potential. It is critical to conduct geological investigation of geothermal resources prior to mining, so as to deepen our knowledge and comprehension of geothermal resources. Ground water is heated by magmatic rocks and geothermal resources can be created in magmatic rock areas. However, their communication is weak, and the depth of burial is typically great. It is difficult for traditional geophysical methods, such as induced polarization method, to achieve useful exploration depths, and they have low accuracy. In this article, a comprehensive geophysical method, based on the controlled source audio-frequency magnetotelluric method (CSAMT) and transient electromagnetic method (TEM), is applied to geothermal exploration in a magmatic rock area. This method compensates for the shortcomings of a single method and achieves a good exploration effect, thereby providing a reliable geological foundation for further development and utilization of geothermal resources.
Investigation on Boron Alpha Nanotube by Studying Their M‐Polynomial and Topological Indices
Authors: Afzal Deeba , Afzal Farkhanda , Hussain Sabir , Chaudhry Faryal , Thapa Dhana Kumari
Published: January 2022

Graph theory provides an effective tool such as graph polynomial and topological indices (TIs) to the chemist to analyze the different chemical structures. TIs are the numerical entity deducted from the molecular structure. TI helps to study the relationship between the physicochemical properties and structure of the chemical compound. In this article, we investigate the boron α‐nanotube by computing its M‐polynomial and then deducing its TI. Results are also shown by plotting the graphs.
Machine Learning-Based Secure Data Acquisition for Fake Accounts Detection in Future Mobile Communication Networks
Authors: Prabhu Kavin B. , Karki Sagar , Hemalatha S. , Singh Deepmala , Vijayalakshmi R. , Thangamani M. , Haleem Sulaima Lebbe Abdul , Jose Deepa , Tirth Vineet , Kshirsagar Pravin R. , Adigo Amsalu Gosu
Published: January 2022

Social media websites are becoming more prevalent on the Internet. Sites, such as Twitter, Facebook, and Instagram, spend significantly more of their time on users online. People in social media share thoughts, views, and facts and create new acquaintances. Social media sites supply users with a great deal of useful information. This enormous quantity of social media information invites hackers to abuse data. These hackers establish fraudulent profiles for actual people and distribute useless material. The material on spam might include commercials and harmful URLs that disrupt natural users. This spam content is a massive problem in social networks. Spam identification is a vital procedure on social media networking platforms. In this paper, we have proposed a spam detection artificial intelligence technique for Twitter social networks. In this approach, we employed a vector support machine, a neural artificial network, and a random forest technique to build a model. The results indicate that, compared with RF and ANN algorithms, the suggested support vector machine algorithm has the greatest precision, recall, and F-measure. The findings of this paper would be useful in monitoring and tracking social media shared photos for the identification of inappropriate content and forged images and to safeguard social media from digital threats and attacks.
The Influence of Interference Layer on the Prediction of Seismic Information of Channel Sedimentary Reservoir
Authors: Bao Xiangsheng , Cai Zhanhu , Wang Qingzhen , Yu Junfeng , Li Yecheng
Published: January 2022

Channel sedimentary reservoirs are the main oil and gas accumulation sites at home and abroad. With the deepening of oil and gas exploration, channel-related oil and gas reservoirs with obvious seismic characteristics have been mostly discovered, and the rest are basically oil and gas reservoirs with unobvious seismic characteristics and difficult to be discovered. The difficulty and risk of discovering these oil and gas reservoirs are on the increase. In order to reduce the exploration risk, it is necessary to form a more reliable time window control theory with a better seismic interpretation technique for channel reservoir reliability. In this paper, 2D forward modeling was used to study the influence of the upper and lower interference layers on the imaging of channel reservoir. When the lithological composition is relatively stable, the degree of interference of the interference layer is closely related to the seismic wavelet, and it is especially susceptible to the main frequency of the seismic wavelet. The lower the main frequency of seismic wavelet, the greater the degree of interference of interference layers on the imaging of channel reservoir, and vice versa. Further analysis on the characteristics of the Ricker wavelet was made and showed that the influence of the upper and lower interference layers on the imaging information of the channel reservoir is concentrated in half apparent major period. Then, considering the dynamic change of distance between the interference layers and the top and bottom interfaces of channel reservoir, the influence of interference layers on the imaging of channel reservoir was studied through 1D forward modeling. The interference area was divided into maximum interference area and main interference area depending on the degree of influence. On this basis, the time window control theory of channel reservoir prediction was clarified. The seismic information which reflects the channel reservoir reliably can be obtained by avoiding the maximum interference area, especially the main interference area.
Interactive Art Design Based on Intelligent Sensors and Information Fusion Technology
Authors: Zhu Yanming , Qiu Tingting , Miao Wei
Published: January 2022

In the era of rapid development of information technology, the application of smart sensors is becoming more and more extensive. All measurement and control equipment need to obtain raw data through sensors, and machines can also obtain various information through sensors. And this information, especially from the perspective of reliability, accuracy, and intelligent interaction, requires higher interactivity in people’s lives. In order to solve the problem that the existing interactive design is difficult and not intelligent enough, and the information fusion is not uniform enough, the results of the design have various deviations. This article intends to design through the use of smart sensors and information fusion technology to make an improvement to its interactive art design.
Valorization of Waste Chicken Feathers: Fabrication and Characterization of Novel Keratin Nanofiber Conduits for Potential Application in Peripheral Nerve Regeneration
Authors: Khumalo Mduduzi , Sithole Bruce , Tesfaye Tamrat , Lekha Prabashni
Published: January 2022

Flexible, porous, biocompatible, and biodegradable tubular keratin nanofibers were fabricated as nerve regeneration conduits. Keratin was extracted from waste chicken feathers and then blended with polyvinyl alcohol and transformed into nanofiber conduits by electrospinning. The nanofiber conduits had average diameters that ranged from 170 to 234 nm. The nanofibers’ average diameter decreased when the keratin content was increased. In contrast, the range of nanofiber diameter distribution narrowed, suggesting that as nanofibers became thin, their numbers increased, thus reducing the interfacial spaces between them. The analysis confirmed the presence of keratin protein in nanofibers, guaranteeing biocompatibility and biodegradation. TGA showed that keratin improved the thermal stability and hydrophilicity of the nanofibers.
Domination Numbers of Amalgamations of Cycles at Connected Subgraphs
Authors: Boonmee Prakassawat , Ma - In Jirapa , Panma Sayan
Published: January 2022

A set S of vertices of a graph G is a dominating set of G if every vertex in V(G) is adjacent to some vertex in S. A minimum dominating set in a graph G is a dominating set of minimum cardinality. The cardinality of a minimum dominating set is called the domination number of G and is denoted by γ(G). Let G1 and G2 be disjoint graphs, H1 be a subgraph of G1, H2 be a subgraph of G2, and f be an isomorphism from H1 to H2. The amalgamation (the glued graph) of G1 and G2 at H1 and H2 with respect to f is the graph obtained by forming the disjoint union of G1 and G2 and then identifying H1 and H2 with respect to f. In this paper, we determine the domination numbers of the amalgamations of two cycles at connected subgraphs.
Detecting User Behavior in Cyber Threat Intelligence: Development of Honeypsy System
Authors: Odemis Murat , Yucel Cagatay , Koltuksuz Ahmet
Published: January 2022

This research demonstrates a design of an experiment of a hacker infiltrating a server where it is assumed that the communication between the hacker and the target server is established, and the hacker also escalated his rights on the server. Therefore, the honeypot server setup has been designed to reveal the correlation of a hacker’s actions with that of the hacker’s experience, personality, expertise, and psychology. To the best of our knowledge, such a design of experiment has never been tested rigorously on a honeypot implementation except for self-reporting tests applied to hackers in the literature. However, no study evaluates the actual data of these hackers and these tests. This study also provides a honeypot design to understand the personality and expertise of the hacker and displays the correlation of these data with the tests. Our Honeypsy system is composed of a Big-5 personality test, a cyber expertise test, and a capture-the-flag (CTF) event to collect logs with honeypot applied in this sequence. These three steps generate data on the expertise and psychology of known cyber hackers. The logs of the known hacker activities on honeypots are obtained through the CTF event that they have participated in. The design and deployment of a honeypot, as well as the CTF event, were specifically prepared for this research. Our aim is to predict an unknown hacker's expertise and personality by analyzing these data. By examining/analyzing the data of the known hackers, it is now possible to make predictions about the expertise and personality of the unknown hackers. The same logic applies when one tries to predict the next move of the unknown hackers attacking the server. We have aimed to underline the details of the personalities and expertise of hackers and thus help the defense experts of victimized institutions to develop their cyber defense strategies in accordance with the modus operandi of the hackers.
Research on the Technology of Small Coal Pillars of Gob-Side Entry Retained in Deep Mines Based on the Roof Cutting for Pressure Unloading in the Lower Key Stratum
Authors: Yang Yongjie , Zhang Yang
Published: January 2022

With the mining depth getting deeper and deeper, surrounding rock control technology has become a difficult issue in the development of modern mining, especially the mining roadways affected by mining. It is very difficult to control the surrounding rock due to the intense mining of the last working faces. In order to alleviate the impact of dynamic pressure on the gob-side entry retained, solve the ventilation difficulties caused by the commonly used method of the gob-side entry retained without leaving coal pillars, and solve the disadvantages of the inability to effectively isolate water and harmful gases, this paper proposes a small coal pillar gob-side entry retained technology based on the key stratum, that is, cutting the key stratum above the top plate, transferring lateral support pressure from the workings further up the seam to the depths of the coal seam, and then protecting gob-side entry retained method. In the final method of precracking and cutting joints by blasting with D-type polytunnel, we directionally cut the lower key stratum above the roadway in the designed direction and position. The theoretical analysis, numerical simulation and field test, and the method of the blasting broken roof pressure release effect are analyzed. Compared to the unloaded pressure method, the relatively complete direct roof is conducive to the maintenance of the surrounding rock on the gob-side entry retained. At the same time, the pressure relief caused by the fracture of the lower key stratum of the coal roof significantly improves the stress environment. The stress is no longer concentrated on the small coal pillars and the gob-side entry retained, and the required roadway support strength is greatly reduced and no longer needs to carry out the construction of roadside support system. This technology reduces labor intensity and production costs.
The Potential Hepatoprotective Effect of Paeoniae Radix Alba in Thioacetamide-Induced Acute Liver Injury in Rats
Authors: Shin Mi-Rae , Lee Se Hui , Roh Seong-Soo
Published: January 2022

Aim. Acute liver injury (ALI) can occur for various reasons by induced inflammation and apoptosis of liver cells including hepatocytes, Kupffer cells, and hepatic stellate cells. Thioacetamide (TAA), which is a classic hepatotoxin, causes oxidative stress, membrane damage, and accumulation of lipid droplets and subsequently provokes consecutive liver injury. In the current study, we tested whether Paeoniae Radix Alba (PR) could alleviate TAA-induced ALI. Methods. Thirty-five male rats were equally separated into five groups. The first group was the normal group, which received distilled water only. The remaining four groups received intraperitoneal TAA (200 mg/kg) for 3 days to induce ALI. The four groups were divided into the control group (no treatment), silymarin-treated, 100 mg/kg PR-treated, and 200 mg/kg PR-treated. The efficacy of PR against hepatotoxicity was evaluated in terms of the serum biochemical index and protein expression associated with inflammation and apoptosis. Moreover, the dissected livers were analyzed by hematoxylin and eosin stain. Results. PR alleviated liver dysfunction as evidenced by decreased levels of aspartate aminotransferase, alanine aminotransferase, and ammonia. Phosphorylated AMP-activated protein kinase (AMPK) and Sirtuin 1 (Sirt1) levels were obviously decreased in the TAA control group, whereas PR reversed these changes. PR also prevented deteriorative effects through inhibition of inflammation and apoptosis via nuclear transcription factor-kappa Bp65 (NF-κBp65) inactivation. Moreover, we found that the hepatoprotective effect of PR pretreatment was mediated by restoration of histopathological changes. Conclusion. PR efficiently blocked both the inflammatory response and apoptosis through activating the AMPK/Sirt1/NF-κBp65 pathway. Therefore, PR is considered a potential therapeutic agent against ALI.
Short Tear Breakup Time Could Exacerbate the Progression of Presbyopia in Women
Authors: Ayaki Masahiko , Negishi Kazuno
Published: January 2022

Purpose. The contributory factors and symptoms for presbyopia progression have not been fully determined. The purpose of the study was to compare presbyopia progression in subjects with short and normal tear breakup time and to explore the severity of common ocular symptoms associated with presbyopia progression. Method. We conducted a clinic‐based, retrospective, cross‐sectional study. Inclusion criteria were bilateral phakic patients aged 40–69 years with best‐corrected distance visual acuity better than 20/30, and exclusion criteria were the use of glaucoma eye drops, any disease affecting vision, or history of ocular surgery. We measured the binocular near add power and compared the results using Kaplan‐Meier analysis. Association between near add power and ocular symptoms was explored. Results. There were 1411 participants (mean age of 50.1 years). There were no significant differences in age, intraocular pressure, spherical equivalent, astigmatism, or anisometropia between the sexes. Kaplan‐Meier analysis indicated that women with short tear breakup time reached the endpoint (near add power of +3.00 D) significantly earlier than those with normal tear breakup time (P = 0.043; Cox‐Mantel test). Eye fatigue was most severe in the group with an add power of 1.25‐2.00 D. Near add power was correlated with hyperopia, astigmatic errors, and anisometropia. Conclusions. This study suggests an exacerbation of presbyopia progression in women with short tear breakup time. Eye fatigue was most severe in those with an add power of 1.25‐2.00 D.
HNF4A Regulates the Proliferation and Tumor Formation of Cervical Cancer Cells through the Wnt/β-Catenin Pathway
Authors: Ma Hong-Mei , Zhang Qian , Yang Xue-Mei , Hu Yan , Zhang Juan , Chen Lin , Zhao Bin , Yang Wen-ting , Xu Rui
Published: January 2022

Hepatocyte nuclear factor 4 alpha (HNF4A) is a transcriptional factor which plays an important role in the development of the liver, kidney, and intestines. Nevertheless, its role in cervical cancer and the underlying mechanism remain unknown. In this study, both immunohistochemistry and western blotting revealed that the expression of HNF4A was downregulated in cervical cancer. Xenograft assays suggested that HN4A could inhibit tumorigenic potential of cervical cancer in vivo. Functional studies illustrated that HNF4A also inhibited the proliferation and viability of cervical cancer cells in vitro. In addition, FACS analysis implied that HNF4A could induce cell cycle arrest from the G0/G1 phase to S phase. Further studies suggested that HNF4A downregulated the activity of the Wnt/β-catenin pathway. Altogether, our data demonstrated that HNF4A inhibited tumor formation and proliferation of cervical cancer cells through suppressing the activity of the Wnt/β-catenin pathway.
Increased Sphingosine Kinase 1 Expression Is Associated with Poor Prognosis in Human Solid Tumors: A Meta-Analysis
Authors: Zhang Chuanmeng , Zhou Chenglin , Xu Jie , Xue Shanshan
Published: January 2022

Background and Aim. Sphingosine kinase 1 (SPHK1) is a key enzyme of sphingolipid metabolism which is involved in the pathogenesis and progression of human cancer. It has been demonstrated to be upregulated in various types of human malignancies. However, the prognostic value of SPHK1 remains unclear. Therefore, we performed this meta-analysis to assess its predictive value in the prognosis of cancer patients. Methods. PubMed, Web of Science, Embase, CNKI, and Wanfang databases were thoroughly searched for eligible studies, in which the relationship between SPHK1 expression and cancer prognosis was evaluated. Hazard ratios (HRs) and 95% confidence intervals (CIs) were pooled to estimate the impact of SPHK1 expression on cancer patients’ survival. Odds ratios (ORs) and 95% CIs were combined to assess the association between SPHK1 expression and clinicopathological characteristics of cancer patients. The certainty of evidence was evaluated by Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) criteria. Results. Thirty studies comprising 32 cohorts with 5965 patients were included in this meta-analysis. The outcomes indicated that elevated SPHK1 expression was associated with worse overall survival (OS) (

HR
=
1.71

, 95% CI: 1.45-2.01,

P
<
0.001

) and disease-free survival (DFS) (

HR
=
1.34

, 95% CI: 1.13-1.59,

P
=
0.001

). What is more, SPHK1 overexpression was significantly correlated with certain phenotypes of tumor aggressiveness, such as clinical stage (

OR
=
2.07

, 95% CI: 1.39-3.09,

P
<
0.001

), tumor invasion (

OR
=
2.16

, 95% CI: 1.47-3.18,

P
<
0.001

), lymph node metastasis (

OR
=
2.04

, 95% CI: 1.71-2.44,

P
<
0.001

), and distant metastasis (

OR
=
3.16

, 95% CI: 2.44-4.09,

P
<
0.001

). The quality of the evidence for both OS and DFS was low. Conclusions. Increased SPHK1 expression is related to poor prognosis in human cancers and may serve as a promising prognostic marker and therapeutic target for malignant patients. However, conclusions need to be treated with caution because of lack of high quality of evidence.
Dapagliflozin Ameliorates the Formation and Progression of Experimental Abdominal Aortic Aneurysms by Reducing Aortic Inflammation in Mice
Authors: Liu Haole , Wei Panpan , Fu Weilai , Xia Congcong , Li Yankui , Tian Kangli , Li Yafeng , Cheng Daxin , Sun Jiaying , Xu Yangwei , Lu Ming , Xu Boyu , Zhang Yali , Wang Rong , Wang Weirong , Xu Baohui , Liu Enqi , Zhao Sihai
Published: January 2022

Background. Dapagliflozin, a sodium glucose transporter protein-2 (SGLT-2) inhibitor, reduces the risk for cardiovascular diseases. However, the influence of dapagliflozin on nondissecting abdominal aortic aneurysms (AAAs) remains unclear. Methods. AAAs were created in male C57BL/6 mice via intra-aortic porcine pancreatic elastase (PPE) infusion. Mice were daily treated with dapagliflozin (1 or 5 mg/kg body weight) or an equal volume of vehicle through oral gavage beginning one day prior to PPE infusion for 14 days. To investigate its translational value, dapagliflozin or vehicle was also administered to mice with existing AAAs in another cohort. Aortic diameters were measured prior to (day 0 for baseline) and 14 days after PPE infusion. After sacrifice, mice aortae were collected, and following histological analyses were performed. Results. Dapagliflozin treatment significantly reduced aneurysmal aortic expansion following PPE infusion as compared to vehicle treatment especially at 5 mg/kg body weight (approximately 21% and 33% decreases in 1 and 5 mg/kg treatment groups, respectively). The dose-dependent attenuation of AAAs by dapagliflozin was also confirmed on histological analyses. Dapagliflozin remarkably reduced aortic accumulation of macrophages, CD4+ T cells, and B cells particularly following dapagliflozin treatment at 5 mg/kg. Dapagliflozin treatment also markedly attenuated medial SMC loss. Though the difference was not significant, dapagliflozin treatment tended to attenuate CD8+ T cells and elastin degradation. Dapagliflozin treatment at 5 mg/kg caused a 53% reduction in neovessel density. Furthermore, dapagliflozin treatment mitigated further progress of existing AAAs. Conclusion. Dapagliflozin treatment ameliorated PPE-induced AAAs by inhibiting aortic leukocytes infiltration and angiogenesis.
Multiple Intestinal Bacteria Associated with the Better Protective Effect of Bifidobacterium pseudocatenulatum LI09 against Rat Liver Injury
Authors: Zha Hua , Si Guinian , Wang Chenyu , Zhang Hua , Li Lanjuan
Published: January 2022

Bifidobacterium pseudocatenulatum LI09 could protect rats from D‐galactosamine‐ (D‐GalN‐) induced liver injury. However, individual difference in the protective effects of LI09 on the liver injury remains poorly understood. The present study is aimed at determining the multiple intestinal bacteria associated with the better protective effect of LI09 against D‐GalN‐induced rat liver injury. Two rat cohorts, i.e., the nonsevere and severe cohorts, were divided based on their liver injury severity. Higher level of ALB and lower levels of ALT, AST, TBA, TB, IL‐5, and MIP‐3α were determined in the nonsevere cohort than the severe cohort. The alpha diversity indices (i.e., observed species, Shannon, and Pielou indices) did not yield significant differences between the intestinal microbiota of the nonsevere and severe cohorts. The intestinal microbiota composition was different between the two cohorts. Ten phylotypes assigned to Bacteroides, Clostridia_UCG‐014, Clostridium Lachnospiraceae, Lachnospiraceae_NK4A136, and Parabacteroides were closely associated with the nonsevere cohort, among which, ASV8_Lachnospiraceae_NK4A136 was the most associated one. At the structure level, two groups of phylotypes with most correlations were determined in the intestinal microbiota networks of the two cohorts. Among them, ASV135_Lachnospiraceae_NK4A136 was the most powerful gatekeeper in the microbiota network of the nonsevere cohort. In conclusion, some intestinal bacteria, e.g., Lachnospiraceae_NK4A136, Parabacteroides, and Clostridium, were associated with the better protective effect of LI09 against D‐GalN‐induced rat liver injury. They were likely to enhance the effectiveness of LI09, and their clinical application deserves further investigation.
A Cellular Automata Model for Heterogeneous Traffic Flow Incorporating Micro Autonomous Vehicles
Authors: Tanveer Muhammad , Kashmiri Faizan Ahmad , Yan Huimin , Wang Tianshi , Lu Huapu
Published: January 2022

Despite the fact that significant research efforts have been made to the traffic flow theory of autonomous vehicles and manual vehicles, few existing studies have incorporated different modes of both vehicles in their analysis. In this study, we develop a cellular automata simulation model to investigate the impact of different modes of autonomous vehicles (autonomous car, autonomous bus, and autonomous micro car) and conventional vehicles (manual car, manual bus, and manual micro car) on the characteristics of traffic flow. A new type of autonomous mode, i.e., autonomous micro car, is investigated in the model to study the effects of this vehicle mode on the overall capacity of the network. Furthermore, two types of lane-changing behavior, i.e., aggressive lane changing and polite lane changing, are incorporated into the model. The results reveal that micro cars (manual and autonomous) have the potential to reduce traffic congestions and increase the capacity or flow rate (vehicles/hour) of the road. Where the average vehicle occupancy is less than 2, if autonomous micro cars are deployed alongside autonomous cars, the flow rate (vehicles/hour) can be increased significantly. The results highlight the significance of the autonomous micro cars to traffic flow, passenger occupancy, and road capacity.
Clinical Value of PPM1G Gene in Survival Prognosis and Immune Infiltration of Hepatocellular Carcinoma
Authors: Xiao Qingyu , Cheng Zhen , Kuang Wenbin , Wu Haijun , Luo Xi , Wang Renling
Published: January 2022

Objective. Liver cancer is one of the most common malignancies, but its prognosis is still poor. Exploring potential biomarkers is an important direction of tumor research. We intend to use bioinformatics methods to explore potential biomarkers related to survival and prognosis of HCC. Methods. The mRNA and protein expressions of PPM1G in liver cancer were analyzed by HPA, TIMER, and UALCAN databases, and the effects of PPM1G on the prognosis of liver cancer patients were explored by the GEPIA database. We also explored the correlation between PPM1G expression and liver cancer immune infiltration through the TIMER database and further explored the potential protein interaction network of PPM1G through the STRING database. Results. The mRNA and protein expression of PPM1G gene in hepatocellular carcinoma tissues was lower than that in normal adjacent tissues. Liver cancer patients with high expression of PPM1G have a better prognosis than those with low expression of PPM1G. The expression of PPM1G is positively or negatively correlated with different immune cells of liver cancer, such as CD4+ T lymphocytes, CD8+ T lymphocytes, B cells, macrophages, and neutrophils. Conclusion. The liver cancer patients with high expression of PPM1G have a good prognosis, and PPM1G gene may be a potential immunotherapy target and prognostic marker of liver cancer.
Application Value of Serum Multi-Antibody Combined Detection in Differential Diagnosis and Typing of Lung Cancer
Authors: Cai Tian , Yao Weishen , Liang Fuyou , Yang Qingshui , Han Fulang
Published: January 2022

One of the most prevalent malignant tumours is lung cancer. Circulating microRNAs (miRNAs) have shown to have significant promise for lung cancer diagnosis and prognosis, according to a growing body of research. The researchers wanted to explore if serum exosomal miR-1246 has any treatment significance in patients with non-small-cell lung cancer (NON-SCLC). Real-time PCR was used to determine the stage of exosomal miR-1246 serum expression in NON-SCLC patients. The researchers next looked into the link regarding exosomal miR-1246 serum stages and NON-SCLC prognosis. In NON-SCLC patients, exosomal miR-1246 serum appearance was considerably higher. According to a receiver operating characteristic (ROC) research, serum exosomal miR-1246 was effective in discriminating NON-SCLC patients from normal controls and non-malignant respiratory illness patients. Following treatment, the amount of serum exosomal miR-1246 reduced but increased in cases of recurrence. Furthermore, the level of serum exosomal miR-1246 was connected to distant metastases and TNM stages in a significant way. According to a survival analysis, cases with severe levels of exosomal miR-1246 serum had reduced overall or disease-free survival. The level of exosomal miR-1246 serum was found to be an autonomous predictive issue for NON-SCLC in multi-variate analysis. Finally, exosomal miR-1246 serum may be a useful prognosis biomarker for non-small-cell lung cancer.
Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway
Authors: Yu Zhaoxia , Liu Ju , Fan Qiqi , Yu Jun , Ren Xiaoting , Wang Xiaobin
Published: January 2022

Nowadays, microRNA-375 (miR-375) has been implicated in many types of cancers, including hepatocellular carcinoma (HCC), and the functions of miRNAs encapsulated by extracellular vesicles (EV) in HCC progression have also been extensively investigated. In this research, we aimed to probe into the mechanism of EV-encapsulated miR-375 from bone marrow-derived mesenchymal stem cells (BM-MSCs) in HCC progression. At first, miR-375 expression in HCC tissues and cells was detected using RT-qPCR, and miR-375 was overexpressed to specify the effects of miR-375 on the malignant phenotype of HCC cells. miR-375 was downregulated in HCC, and overexpression of miR-375 suppressed HCC cell growth. Then, BM-MSCs and EV were isolated and identified, and, EV were cocultured with HCC cells for further functional assays. It was found that miR-375 encapsulated by EV could restrict the malignant phenotypes of HCC cells. Furthermore, the downstream genes and signaling cascades involved in HCC growth were investigated. HOXB3 was determined to be a downstream target of miR-375, and upregulation of miR-375 decreased Wnt1 and β-catenin protein expression. Furthermore, HOXB3 blocked the repressive effects of miR-375 on HCC cells and Wnt1 and β-catenin expression. This study highlights that miR-375 encapsulated by EV inhibits HCC development via modulating the HOXB3/Wnt/β-catenin axis.
Analysis of Dynamic Damage Fractal and Energy Consumption Characteristics of Dolomite Marble
Authors: Zuo Ting , Zhao Zehu , Wang Jianguo , Li Xianglong , Tao Zihao , Yang Changhui
Published: January 2022

To investigate the damage fractal and energy dissipation of dolomite marble under blasting load, Dahongshan Copper Mine was selected as the test object and one-dimensional SHPB impact tests with different strain rates were carried out. The dynamic compressive strength, damage morphology, fractal fragmentation, and energy dissipation of the rock were analyzed. The results showed that the absorbed energy per unit volume and the crushing energy per unit mass of dolomite marble are closely related to the dynamic compressive strength. The fractal dimension D of dolomite marble increases with increasing strain rate, and the smaller the average block size of the rock, the degree of crushing deepens. In addition, as the incident energy increases, the crushing energy of the rock also increases, and the energy dissipation intensifies. Finally, after comparative analysis, the reasonable strain rate range is 68.14–77.61 s−1, and the reasonable specific energy absorption value range is 0.65–0.98 J/cm3. The fractal pattern of rock deformation and damage can be reasonably explained from the perspective of energy dissipation.
LINC02273 Promotes Hepatocellular Carcinoma Progression via Retaining β‐Catenin in the Nucleus to Augment Wnt Signaling
Authors: Peng Liang , Ye Rong , Zhu Xiansen , Xie Yuankang , Zhong Baiyin , Liu Yao , Li Heping , Xie Binhui
Published: January 2022

Hepatocellular carcinoma (HCC) is a lethal malignancy whereas the molecular mechanisms remain poorly understood. Recently, long noncoding RNAs (lncRNA) have been shown to regulate HCC progression. However, the involved lncRNAs remain to be fully explored. Here, we showed the expression pattern and biological function of a recently identified lncRNA, LINC02273, in HCC. LINC02273 played a critical role in HCC progression via stabilizing β‐catenin. Knockdown of LINC02237 remarkably inhibited the proliferation, stemness, migration, and invasion abilities, whereas it increased the apoptosis of HCC cells. Overall, we characterized the functions of LINC02273 in HCC and its potential as a novel HCC targeting candidate.
Problems and Solutions of Art Professional Service Rural Revitalization Strategy Based on Random Forest Algorithm
Authors: Liang Jianhui
Published: January 2022

The rural revitalization strategy proposal is not a passive water, but rather one with its own internal historical logic. The rural revitalization strategy has been implemented in a consistent manner with China’s rural construction practice over the last 100 years. Since modern times, the Chinese agricultural civilization has been severely harmed by the collision and friction with western industrial and commercial civilizations, which has directly aroused the strong reflection of people with lofty ideals from all walks of life in Chinese society and led them down the path of rural construction aimed at “rejuvenating” the countryside. Accelerating the development of rural culture is beneficial to the overall improvement of social culture and the happiness of the Chinese people. As a result, the development and construction of rural culture will have a significant impact on China. The random forest algorithm simulates the data learning process based on data mining, creates a model, and returns a judgment result. The strategy of art professional service is examined in this study. The random forest algorithm was used to revitalize rural areas. The random forest algorithm’s learning process is very quick, and it is still very effective in dealing with art professional services. The current random forest algorithm weighs the importance of all variables to determine the significance of switching from independent to dependent variables.
Identification of MAD2L1 as a Potential Biomarker in Hepatocellular Carcinoma via Comprehensive Bioinformatics Analysis
Authors: Chen Qian , Yang Sibo , Zhang Yewei , Li Bo , Xu Huanming , Zuo Shi
Published: January 2022

Background. Hepatocellular carcinoma (HCC) is widely acknowledged as a malignant tumor with rapid progression, high recurrence rate, and poor prognosis. At present, there is a paucity of reliable biomarkers at the clinical level to guide the management of HCC and improve patient outcomes. Our research is aimed at assessing the prognostic value of MAD2L1 in HCC. Methods. Four datasets, GSE121248, GSE101685, GSE85598, and GSE62232, were selected from the GEO database to analyze differentially expressed genes (DEGs) between HCC and normal liver tissues. After functional analysis, we constructed a protein‐protein interaction network (PPI) for DEGs and identified core genes in this network with high connectivity with other genes. We assessed the relationship between core genes and the pathogenesis and prognosis of HCC. Finally, we explored the gene regulatory signaling mechanisms involved in HCC pathogenesis. Results. 145 DEGs were screened from the intersection of the four GEO datasets. MAD2L1 was associated with most genes according to the PPI network and was selected as a candidate gene for further study. Survival analysis suggested that high MAD2L1 expression in HCC correlated with a worse prognosis. In addition, real‐time quantitative PCR (RT‐qPCR), western blot (WB), and immunohistochemistry (IHC) findings suggested that the expression of MAD2L1 was abnormally increased in HCC tissues and cells compared to paraneoplastic tissues and normal hepatocytes. Conclusion. We found that high MAD2L1 expression in HCC was significantly associated with overall patient survival and clinical features. We also explored the potential biological properties of this gene.
A Mixture of Ginkgo biloba L. Leaf and Hericium erinaceus (Bull.) Pers. Fruit Extract Attenuates Scopolamine-Induced Memory Impairments in Mice
Authors: Hong Seong Min , Yoon Da Hye , Lee Mi Kyeong , Lee Jae Kang , Kim Sun Yeou
Published: January 2022

Alzheimer’s disease (AD) is a neurodegenerative disease that is characterized by loss of memory and cognitive impairment via dysfunction of the cholinergic nervous system. In cholinergic dysfunction, it is well known that impaired cAMP response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) signaling are major pathological markers and are some of the strategies for the development of AD therapy. Therefore, this study is aimed at evaluating whether a mixture comprising Ginkgo biloba L. leaf (GL) and Hericium erinaceus (Bull.) Pers. (HE) fruit extract (GH mixture) alleviated cognitive impairment induced in a scopolamine-induced model. It was discovered that GH reduced neuronal apoptosis and promoted neuronal survival by activating BDNF signaling in an in vitro assay. In addition, the GH (p.o. 240 mg/kg) oral administration group significantly restored the cognitive deficits of the scopolamine-induced mouse group (i.p. 1.2 mg/kg) in the behavior tests such as Y-maze and novel object recognition task (NORT) tests. This mixture also considerably enhanced cholinergic system function in the mouse brain. Furthermore, GH markedly upregulated the expressed levels of extracellular signal-regulated kinase (ERK), CREB, and BDNF protein levels. These results demonstrated that GH strongly exerted a neuroprotective effect on the scopolamine-induced mouse model, suggesting that an optimized mixture of GL and HE could be used as a good material for developing functional foods to aid in the prevention of neurodegenerative diseases, including AD.
Phase I Trial of Cetuximab, Radiotherapy, and Ipilimumab in Locally Advanced Head and Neck Cancer
Authors: Ferris Robert L. , Moskovitz Jessica , Kunning Sheryl , Ruffin Ayana T. , Reeder Carly , Ohr James , Gooding William E. , Kim Seungwon , Karlovits Brian J. , Vignali Dario A.A. , Duvvuri Umamaheswar , Johnson Jonas T. , Petro Daniel , Heron Dwight E. , Clump David A. , Bruno Tullia C. , Bauman Julie E.
Published: January 2022

Purpose:

Concurrent radiotherapy with cetuximab, an anti-EGFR mAb, is a standard treatment for locally advanced head and neck squamous carcinoma (HNSCC). Cytotoxic T lymphocyte antigen-4–positive (CTLA-4+) regulatory T cells (Treg) dampen cellular immunity and correlate negatively with clinical outcomes. This phase I study added ipilimumab, an anti–CTLA-4 mAb, to cetuximab-radiotherapy.

Patients and Methods:

A (3 + 3) design was used to establish the recommended phase II dose (RP2D) of ipilimumab, added at week 5 for four, every-3-week doses to fixed, standard cetuximab-radiotherapy. Eligible subjects had stage III to IVb, high-risk [human papillomavirus–negative (HPV−)] or intermediate-risk HPV-positive (HPV+)] HNSCC. Dose-limiting toxicity (DLT) was defined as any grade 4 adverse event (AE) except in-field radiation dermatitis or immune-related (ir) AE requiring ≥2 weeks of systemic steroids. Baseline tumor and serial blood specimens were collected for immune correlatives.

Results:

From July 2013 to May 2016, 18 patients enrolled. Two of 6 in cohort 1 (ipilimumab 3 mg/kg) experienced grade 3 dermatologic DLTs, triggering deescalation of ipilimumab to 1 mg/kg. Dose level -1 was expanded to N  =  12 without DLT. irAE included: grade 1, 2, and 3 dermatitis (2, 1, and 3 cases), grade 4 colitis (1), and grade 1 hyperthyroidism (1). Three-year disease-free survival (DFS) and overall survival were 72% [90% confidence interval (CI), 57–92] and 72% (90% CI, 56–92). High expression of coinhibitory receptors PD1/LAG3/CD39 on baseline tumor-infiltrating Treg was associated with worse DFS (HR = 5.6; 95% CI, 0.83–37.8; P = 0.08).

Conclusions:

The RP2D for ipilimumab plus standard cetuximab–radiotherapy is 1 mg/kg in weeks 5, 8, 11, and 14. The regimen is tolerable and yields acceptable survival without cytotoxic chemotherapy.
Phase I Clinical Trial of DNA Methyltransferase Inhibitor Decitabine and PARP Inhibitor Talazoparib Combination Therapy in Relapsed/Refractory Acute Myeloid Leukemia
Authors: Baer Maria R. , Kogan Aksinija A. , Bentzen Søren M. , Mi Tian , Lapidus Rena G. , Duong Vu H. , Emadi Ashkan , Niyongere Sandrine , O'Connell Casey L. , Youngblood Benjamin A. , Baylin Stephen B. , Rassool Feyruz V.
Published: January 2022

Purpose:

Patients with acute myeloid leukemia (AML) unfit for, or resistant to, intensive chemotherapy are often treated with DNA methyltransferase inhibitors (DNMTi). Novel combinations may increase efficacy. In addition to demethylating CpG island gene promoter regions, DNMTis enhance PARP1 recruitment and tight binding to chromatin, preventing PARP-mediated DNA repair, downregulating homologous recombination (HR) DNA repair, and sensitizing cells to PARP inhibitor (PARPi). We previously demonstrated DNMTi and PARPi combination efficacy in AML in vitro and in vivo. Here, we report a phase I clinical trial combining the DNMTi decitabine and the PARPi talazoparib in relapsed/refractory AML.

Patients and Methods:

Decitabine and talazoparib doses were escalated using a 3 + 3 design. Pharmacodynamic studies were performed on cycle 1 days 1 (pretreatment), 5 and 8 blood blasts.

Results:

Doses were escalated in seven cohorts [25 patients, including 22 previously treated with DNMTi(s)] to a recommended phase II dose combination of decitabine 20 mg/m2 intravenously daily for 5 or 10 days and talazoparib 1 mg orally daily for 28 days, in 28-day cycles. Grade 3–5 events included fever in 19 patients and lung infections in 15, attributed to AML. Responses included complete remission with incomplete count recovery in two patients (8%) and hematologic improvement in three. Pharmacodynamic studies showed the expected DNA demethylation, increased PARP trapping in chromatin, increased γH2AX foci, and decreased HR activity in responders. γH2AX foci increased significantly with increasing talazoparib doses combined with 20 mg/m2 decitabine.

Conclusions:

Decitabine/talazoparib combination was well tolerated. Expected pharmacodynamic effects occurred, especially in responders.