bluuebunny/crossref_metadata_2025 huggingface dataset:
Strain-level characterization of foodborne pathogens without culture enrichment for outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
Authors: Buytaers Florence E. , Verhaegen Bavo , Van Nieuwenhuysen Tom , Roosens Nancy H. C. , Vanneste Kevin , Marchal Kathleen , De Keersmaecker Sigrid C. J.
Published: March 2024

Introduction

Shotgun metagenomics has previously proven effective in the investigation of foodborne outbreaks by providing rapid and comprehensive insights into the microbial contaminant. However, culture enrichment of the sample has remained a prerequisite, despite the potential impact on pathogen detection resulting from the growth competition. To circumvent the need for culture enrichment, we explored the use of adaptive sampling using various databases for a targeted nanopore sequencing, compared to shotgun metagenomics alone.

Methods

The adaptive sampling method was first tested on DNA of mashed potatoes mixed with DNA of a Staphylococcus aureus strain previously associated with a foodborne outbreak. The selective sequencing was used to either deplete the potato sequencing reads or enrich for the pathogen sequencing reads, and compared to a shotgun sequencing. Then, living S. aureus were spiked at 105 CFU into 25 g of mashed potatoes. Three DNA extraction kits were tested, in combination with enrichment using adaptive sampling, following whole genome amplification. After data analysis, the possibility to characterize the contaminant with the different sequencing and extraction methods, without culture enrichment, was assessed.

Results

Overall, the adaptive sampling outperformed the shotgun sequencing. While the use of a host removal DNA extraction kit and targeted sequencing using a database of foodborne pathogens allowed rapid detection of the pathogen, the most complete characterization was achieved when using solely a database of S. aureus combined with a conventional DNA extraction kit, enabling accurate placement of the strain on a phylogenetic tree alongside outbreak cases.

Discussion

This method shows great potential for strain-level analysis of foodborne outbreaks without the need for culture enrichment, thereby enabling faster investigations and facilitating precise pathogen characterization. The integration of adaptive sampling with metagenomics presents a valuable strategy for more efficient and targeted analysis of microbial communities in foodborne outbreaks, contributing to improved food safety and public health.
Developmental patterns of extracellular matrix molecules in the embryonic and postnatal mouse hindbrain
Authors: Wéber Ildikó , Dakos Adél , Mészár Zoltán , Matesz Clara , Birinyi András
Published: March 2024

Normal brain development requires continuous communication between developing neurons and their environment filled by a complex network referred to as extracellular matrix (ECM). The ECM is divided into distinct families of molecules including hyaluronic acid, proteoglycans, glycoproteins such as tenascins, and link proteins. In this study, we characterize the temporal and spatial distribution of the extracellular matrix molecules in the embryonic and postnatal mouse hindbrain by using antibodies and lectin histochemistry. In the embryo, hyaluronan and neurocan were found in high amounts until the time of birth whereas versican and tenascin-R were detected in lower intensities during the whole embryonic period. After birth, both hyaluronic acid and neurocan still produced intense staining in almost all areas of the hindbrain, while tenascin-R labeling showed a continuous increase during postnatal development. The reaction with WFA and aggrecan was revealed first 4th postnatal day (P4) with low staining intensities, while HAPLN was detected two weeks after birth (P14). The perineuronal net appeared first around the facial and vestibular neurons at P4 with hyaluronic acid cytochemistry. One week after birth aggrecan, neurocan, tenascin-R, and WFA were also accumulated around the neurons located in several hindbrain nuclei, but HAPLN1 was detected on the second postnatal week. Our results provide further evidence that many extracellular macromolecules that will be incorporated into the perineuronal net are already expressed at embryonic and early postnatal stages of development to control differentiation, migration, and synaptogenesis of neurons. In late postnatal period, the experience-driven neuronal activity induces formation of perineuronal net to stabilize synaptic connections.
Management of intervenable factors to reduce vascular complications in patients with internal carotid artery occlusion treated by non-emergency endovascular treatment
Authors: Wu Guangyu , Nong Yuxin , Hong Shaorui , Wang Shuo , Dai Chengbo , He Chizhong , Li Changmao , Ma Tengyun , Yang Zhexian , Zhang Bin , Gao Yuyuan , Ma Guixian
Published: March 2024

Objective

This study aims to identify risk factors for vascular complications during non-emergency endovascular treatment in patients with internal carotid artery occlusion (ICAO) and to propose potential interventions.

Method

A retrospective analysis of 92 patients with ICAO who received non-emergency endovascular treatment in our center from 1 January 2018 to 31 June 2023, was conducted. The correlation between intraoperative vascular complications and potential risk factors was studied, and interaction analysis was performed.

Results

Our findings revealed that the use of non-neurology guide wires to open vessels (adjusted OR: 4.1, 95%CI: 1.3–12.8; p = 0.014) and glycosylated hemoglobin (HbA1c) ≥ 6.5 mmol/L (adjusted OR: 3.2, 95%CI: 1.2–8.9; p = 0.023) was significantly associated with vascular complications in non-emergency endovascular treatment of ICAO patients. The restricted cubic spline (RCS) showed that the higher the HbA1c level, the higher the risk of vascular complications.

Conclusion

The use of non-neurology guide wires for vessel opening during non-emergency endovascular treatment in patients with ICAO increases the risk of vascular complications. Preoperative assessment and management of HbA1c levels can reduce the incidence of intraoperative vascular complications.
Renal cell oncocytoma tumor evolutions: a case report
Authors: Alduraibi Alaa Khalid , Essa Noora Bin , Bomanji Jamshed
Published: March 2024

This report delineates the clinical progression of a renal oncocytoma in a 38-year-old female, initially asymptomatic, over a three-year follow-up period following her treatment for papillary thyroid cancer. The timeline of this case is as follows: In 2016, the patient was treated with total thyroidectomy and I131 for thyroid cancer. During an annual follow-up, an incidental renal mass was detected via FDG PET/CT, initially characterized as a benign, non-FDG-avid renal oncocytoma. Over two years, this lesion demonstrated a remarkable increase in FDG uptake and a slight growth in size, coupled with new I131 uptake in subsequent scans. These findings led to a reassessment of the diagnosis, initially suggesting a potential small renal cell carcinoma (RCC). Histopathological analysis eventually confirmed the diagnosis of oncocytoma. This case is notable for the tumor’s unusual metabolic evolution and the challenges it posed in differential diagnosis.
Genetic alterations in myeloid sarcoma among acute myeloid leukemia patients: insights from 37 cohort studies and a meta-analysis
Authors: Untaaveesup Suvijak , Trithiphen Sasinipa , Kulchutisin Kamolchanok , Rungjirajittranon Tarinee , Leelakanok Nattawut , Panyoy Sujitra , Kaokunakorn Thanapon , Owattanapanich Weerapat
Published: March 2024

Introduction

Variations in mutation rates among acute myeloid leukemia (AML) patients with myeloid sarcoma (MS) underscore the need for a thorough examination. This meta-analysis was conducted to fill the information gap concerning mutation frequencies in AML patients presenting with MS.

Materials and methods

This study included retrospective and prospective cohorts. It examined genetic alterations in AML patients with and without MS across all age groups. The search strategy employed terms such as “acute myeloid leukemia,” “extramedullary,” “granulocytic sarcoma,” “myeloid sarcoma,” and “leukemic cutis” in the EMBASE, MEDLINE, and Scopus databases. Excluded from the study were reviews, case reports, and case series with fewer than 10 cases. Statistical analyses were performed with Review Manager 5.4 software.

Results

The primary analysis incorporated data from 37 cohorts involving 5646 diagnosed AML patients and revealed a 17.42% incidence of MS. The most prevalent mutation among AML patients with MS was FLT3-ITD, with a pooled prevalence of 17.50% (95% CI 12.60% to 22.50%; I2 82.48%). The dominant fusion gene was RUNX1::RUNX1T1, displaying a pooled prevalence of 28.10% (95% CI 15.10% to 41.20%; I2 96.39%). In comparison, no significant intergroup differences were observed for NPM1, FLT3-ITD, KIT, and IDH2 mutations. Interestingly, the CEBPA mutation exhibited protective effects for MS patients, with an odds ratio of 0.51 (95% CI 0.32 to 0.81; I2 0%). Conversely, the NRAS mutation was associated with an increased risk of MS development, with an odds ratio of 5.07 (95% CI 1.87 to 13.73; I2 0%).

Conclusion

This meta-analysis sheds light on the prevalence of genetic mutations in AML patients with MS, providing insights into the unique characteristics of the mutations and their frequencies. These discoveries are crucial in informing therapeutic and prognostic decisions for individuals with myeloid sarcoma.
Case report and literature review: plexiform schwannoma in the penile and inguinal region in a child
Authors: Qi Xiaoying , Tan Yifei , Feng Yanru , Ma Dan , Wang Ling , Pang Houqing
Published: March 2024

Penile schwannoma is an uncommonly seen peripheral nerve tumor, of which penile plexiform schwannomas (PS) is extremely rare that has only been reported in several adults. We present a case of penile PS with a similar lesion in inguinal region in a 9-year-old child, which appeared as painless masses and rapidly growing within one year. Penile ultrasonography suggested well-defined lesions with limited vascularity. Both masses presented with low-to-intermediated signal intensity and no definite enhancement in computed tomography. The lesions were completely resected with minimal intraoperative bleeding, and a diagnose of benign PS was confirmed based on H&E staining and positive S-100 expression in immunohistochemistry. There was no evidence of tumor recurrence or metastasis after 6 months of follow-up. Only 6 cases of penile schwannoma in children were recorded, of which 5 were malignant, and none was PS. The malignancy rate of penile schwannoma in children may be overestimated due to delayed diagnose of benign ones. A rapidly growing penile mass with a suspected metastatic lesion in inguinal region could be easily misdiagnosed as malignant. This case report and literature review is expected to assist clinicians in getting a comprehensive understanding of children penile schwannomas and choosing the best management strategy when faced with this rare condition.
Burkitt's lymphoma in a young boy progressing to systemic lupus erythematosus during follow-up: a case report and literature review
Authors: Liu Chenxi , Pan Ci , Jin Yingying , Huang Hua , Ding Fei , Xu Xuemei , Bao Shengfang , Han Xiqiong , Jin Yanliang
Published: March 2024

Introduction

Patients with systemic lupus erythematosus (SLE) are at a higher risk of developing cancer, particularly hematological malignancies such as lymphoma and leukemia. However, existing studies on this topic that assess cancer incidence following SLE diagnosis are limited. In addition, SLE can be diagnosed after cancer, although such cases in children have been rarely reported.

Case report

We present the case of a 2.6-year-old boy who presented to our institute with fever and abdominal pain. His physical examination revealed a periumbilical mass, which was pathologically diagnosed as Burkitt's lymphoma. Autologous stem cell transplantation was performed to consolidate the effect of chemotherapy and reduce the risk of cancer relapse. He was diagnosed with SLE 5 years later, following the presentation of a fever with rash, positive autoantibodies, decreased complement, and kidney involvement. At the final follow-up, the patient was still alive and showed no recurrence of Burkitt's lymphoma or disease activity of SLE.

Conclusion

Despite the low frequency of SLE in children with lymphoma, cancer and SLE may be induced by a common mechanism involving B-cell cloning and proliferation. Therefore, hematologists and rheumatologists should be aware of the occurrence of these two conditions during patient follow-up.
Optimization and preparation of a compound cod liver oil film former agent: an orthogonal design
Authors: He Yao , Zhang Ying , Li Zheng
Published: March 2024

Background: Cod liver oil has anti-inflammatory properties and could help regulate recurrent aphthous stomatitis (RAS). An orthogonal experiment was used to evaluate and improve the dosage form of compound cod liver oil, which has replaced the previously used liniment preparation based on film method.

Methods: An orthogonal experiment was adopted, and the appearance and film-forming time of the film coating agents were used as indicators. The optimal ratio in the preparation process for the compound cod liver oil film agent was then optimized. A method for determination of compound cod liver oil film was established using High-Performance Liquid Chromatography (HPLC).

Results: The results indicate that the blank films prepared using 55 mg polyvinyl alcohol (PVA) (PVA low), 45 mg of PVA (PVA medium), and 10 mg glycerol had the optimal performance, which was defined as PVAa. The drug-carrying film prepared from 3 mL PVAa (i.e., film-forming material with the optimal proportion), 30 mg dexamethasone acetate, and 30 mg metronidazole had the optimal performance. The verified sample has a complete and smooth appearance, uniform thickness and color, and no evident bubbles, which meets the requirements for a film agent defined in the Chinese Pharmacopoeia, 2020 edition. HPLC was used to determine the major components: dexamethasone acetate, metronidazole, and dyclonine hydrochloride, and the optimal separation effect was obtained. The method has advantages of good specificity, good linear results, high recovery rate, and good repeatability.

Conclusion: This study proposes an optimized compound cod liver oil film former agent and preparation method. The results indicate that the compound cod liver oil film former agent had good performance, reflecting the high feasibility of this research method. The detection method of compound cod liver oil film was established by HPLC. The method was feasible, and the validity and stability of the formulation and preparation technology were guaranteed. The role of the newly developed agent in patients with RAS should be investigated further.
The effects of Massa Medicata Fermentata on the digestive function and intestinal flora of mice with functional dyspepsia
Authors: Wang Shuyu , Li Yuanlin , Yang Xiaoqi , Hao Yinxue , Zhan Xueyan
Published: March 2024

Introduction: The purpose of this study was to identify the chemical components of Massa Medicata Fermentata (MMF) in different fermentation methods, analyze its regulatory effects on gastrointestinal propulsion and intestinal flora in mice with food accumulation, and further explore its mechanism of action in the treatment of dyspepsia.

Methods: The chemical compositions of three kinds of MMF were identified using the UPLC-Q- Exactive Orbitrap mass spectrometer. A model of spleen deficiency and food accumulation in mice was established. The gastric emptying rate and intestinal propulsion rate were calculated, serum gastrin concentration and cholinesterase activity were measured, and 16S rRNA microbial detection was performed in different groups of mouse feces.

Results: The results showed that a total of 95 chemical components were identified from the three MMF extracts, 62 of which were the same, but there were differences in flavonoids and their glycosides, organic acids, and esters. MMF, PFMMF, and commercial MMF could all significantly improve the gastric emptying rate, intestinal propulsion rate, and GAS concentration in the serum of model mice; PFMMF has a better effect, while there was no significant difference in cholinesterase activity among the groups (p > 0.05). The 16S rRNA sequencing results showed that the MMF and PFMMF could increase the content of beneficial bacteria Bacteroidetes and decrease the pathogenic bacteria Verrucomicrobia in the intestines of model mice, while the commercial MMF could not.

Discussion: Studies suggest that MMF has a variety of possible mechanisms for improving food accumulation and treating gastrointestinal dyspepsia, which provides reference value for the quality evaluation and clinical application of MMF.
Transient submillimeter-scale periodic banding of Ag2O precipitate through reaction–transport–reaction processes
Authors: Hayashi Hisashi , Yamada Kaho
Published: March 2024

We describe a new class of the periodic banding of Ag2O precipitates through reaction–transport–reaction processes in an agarose-hydrogel column; the gel contains NaNO3 as an additive and is inserted between Ag and Ti rods with an applied constant voltage of 4.0–7.0 V. Submillimeter-scale periodic dark-brown precipitation bands were transiently generated with high reproducibility in this system, in which (i) electrochemical reactions at the metal rods to generate Ag+ and OH−, (ii) transportation of the reactant ions by the electric field, and (iii) reactions to produce Ag2O are expected to couple. The bands successively emerged mainly toward the cathode, accompanied by substantial changes in the electric current through the gel. The periodic banding depended on the applied voltage, duration of application, and concentration of NaNO3 initially loaded in the gel. The banding was most clearly observed in a 2.0 mass% agarose gel column containing 0.005 M NaNO3 under a constant applied voltage of ∼5.5 V for ∼3 h. This optimal condition resulted in more than ten periodic bands, of which the bandwidth (w) and the distance between the adjacent bands (d) were randomly distributed around the average values: for the bands formed up to 2 h, w = 0.25 ± 0.04 mm and d = 0.42 ± 0.05 mm; for the bands formed after 2 h, w = 0.42 ± 0.11 mm and d = 0.68 ± 0.07 mm. The generated periodic bands were gradually painted out with time to form an almost continuous broad band, even after the applied voltage was discontinued, although immersion of the gel in deionized water for 3 h could suppress this uniformization. Observations using optical and scanning electron microscopes with an energy-dispersive X-ray detector suggested that the periodic bands were composed of gelatinous Ag2O and micrometer-scale (1–50 μm) clusters, which comprised Ag-abundant nanoparticles with considerable size dispersion (10–500 nm). The relationship between the periodic banding and inhomogeneous distribution of the reactant ions and potential applications of the observed banding phenomena are discussed.
Efficient 10 ns-scale 2-μm optical parametric oscillator based on 1064 nm pump source
Authors: Chen Si-Ying , Yang Yun-Fei , Li Yun-Fei , Wang Gong , Yu Yu , Du Hai-Yang , Cao Feng-Li , Yin Hong-Wei , Zhu Chen-Yu , Wang Yu-Lei , Lu Zhi-Wei
Published: March 2024

Lasers operating around 2-µm have been extensively researched for various applications, such as LiDAR, remote sensing, optical communication, and medicine. One of the approaches to generate 2-µm emission is to employ an optical parametric oscillator pumped by 1-µm laser; however, improving the 2-µm laser conversion efficiency of optical parametric oscillators is still a challenge. In this study, we exploit the high nonlinear coefficient of the KTP crystal at the 2-µm band to realize a high-efficiency optical parametric oscillator. The OPO is driven by a laser diode (LD) side-pumped, electro-optic Q-modulated Nd:YAG laser. The cavity was designed and optimized based on mode matching to increase the peak power density of the pump laser. Different powers were obtained for different pump laser focusing conditions, and the maximum power was obtained when the KTP crystal was placed in front of the pump laser focus. The output characteristics of the KTP at different phase-matching angles were also investigated. An output power of 2.44 W and an output pulse width of 9.04 ns were obtained at 2154.4 nm with an optical-to-optical conversion efficiency of 23.39% and a slope efficiency of 36.09%.
Lipid droplets in Arabidopsis thaliana leaves contain myosin-binding proteins and enzymes associated with furan-containing fatty acid biosynthesis
Authors: Omata Yuto , Sato Reina , Mishiro-Sato Emi , Kano Keiko , Ueda Haruko , Hara-Nishimura Ikuko , Shimada Takashi L.
Published: March 2024

Lipid droplets (LDs) are lipid storage organelles in plant leaves and seeds. Seed LD proteins are well known, and their functions in lipid metabolism have been characterized; however, many leaf LD proteins remain to be identified. We therefore isolated LDs from leaves of the leaf LD–overaccumulating mutant high sterol ester 1 (hise1) of Arabidopsis thaliana by centrifugation or co-immunoprecipitation. We then performed LD proteomics by mass spectrometry and identified 3,206 candidate leaf LD proteins. In this study, we selected 31 candidate proteins for transient expression assays using a construct encoding the candidate protein fused with green fluorescent protein (GFP). Fluorescence microscopy showed that MYOSIN BINDING PROTEIN14 (MYOB14) and two uncharacterized proteins localized to LDs labeled with the LD marker. Subcellular localization analysis of MYOB family members revealed that MYOB1, MYOB2, MYOB3, and MYOB5 localized to LDs. LDs moved along actin filaments together with the endoplasmic reticulum. Co-immunoprecipitation of myosin XIK with MYOB2-GFP or MYOB14-GFP suggested that LD-localized MYOBs are involved in association with the myosin XIK–LDs. The two uncharacterized proteins were highly similar to enzymes for furan fatty acid biosynthesis in the photosynthetic bacterium Cereibacter sphaeroides, suggesting a relationship between LDs and furan fatty acid biosynthesis. Our findings thus reveal potential molecular functions of LDs and provide a valuable resource for further studies of the leaf LD proteome.
Comparative efficacy of non-electric cooling techniques to reduce nutrient solution temperature for the sustainable cultivation of summer vegetables in open-air hydroponics
Authors: Nisar Muhammad Mohsin , Mahmood Rashid , Tayyab Salman , Anees Moazzam , Nadeem Faisal , Bibi Sadia , Waseem Faiza , Ahmed Nazir , Li Jing , Song Zhao
Published: March 2024

The cultivation of summer vegetables in open-air nutrient film technique (NFT) hydroponics is limited due to the elevated nutrient solution temperature (NST). In this regard, non-electric evaporative-cooling techniques were explored to maintain NST in open-air NFT hydroponics. Four cooling setups were employed by wrapping polyvinyl chloride (PVC) grow pipes with one and two layers of either wet or dry jute fabrics and attaching them with coiled aluminum pipe buried inside a) wet sand-filled brick tunnels (Cooling Setup I), b) two inverted and vertically stacked earthen pots (Cooling Setup II), c) two inverted and vertically stacked earthen pots externally wrapped with wet jute fabric (Wrapped Cooling Setup II), and d) an earthen pitcher wrapped with wet jute fabric (Cooling Setup III). Wrapping grow pipes with two layers of wet jute fabric reduced NST by 5°C as compared to exposed (naked) grow pipes. The double-layer jute fabric-wrapped grow pipes produced 182% more reduction in NST in comparison to single layer-wrapped grow pipes. Additionally, the installation of Wrapped Cooling Setup II and Cooling Setup III outperformed Cooling Setup I and Cooling Setup II through NST reduction of approximately 4°C in comparison to control. Interestingly, Cooling Setup III showed its effectiveness through NST reductions of 193%, 88%, and 23% during 11 a.m.–12 p.m. as compared to Cooling Setup I, Cooling Setup II, and Wrapped Cooling Setup II, respectively. In contrast, Wrapped Cooling Setup II caused NST reductions of 168%, 191%, and 18% during 2–3 p.m. in comparison to Cooling Setup I, Cooling Setup II, and Cooling Setup III, respectively. Thus, the double-layer jute fabric-wrapped grow pipes linked with Wrapped Cooling Setup II can ensure summer vegetable cultivation in open-air NFT hydroponics as indicated by the survival of five out of 12 vegetable plants till harvest by maintaining NST between 26°C and 28°C.
Evidence from Albania: local government, public goods, and the free-rider problem
Authors: Lipi Rudina , Lipi Ilirjan , Kumi Evis , Leskaj Etleva
Published: March 2024

Public goods and their benefits are necessarily related to their nature. From an economic point of view, citizens are always looking for more and more public goods, judging that they should always finance them even less. Especially in a democracy, it is sufficient to remember that people who don't vote still benefit from the government's agenda. Mainly in developing countries and especially in ex-communist ones, the public does not have enough literacy and interest to understand the issues of public finance, political economy, the fiscal system, legal and political systems, social paradigms, and economics. So, it generally attributes the provision of public goods necessarily to the central and local governments without understanding or knowing any of the elements of the public goods ecosystem. So it seems that everything that the local government provides must be of high quality and be free, which proves evidence and traces of one of the main problems of public goods, which is the free-rider problem. From the research point of view, regardless of the moderation made by the civil society for the awareness of the social contract as well as the transparency of the economic activity of the municipalities, the citizens' awareness about the nature and problems of the provision of public goods still remains a concern. Therefore, the purpose of this article is to address the free-rider problem as an issue that must be recognized and considered so that optimal local legal regulations can be formulated, especially in small communities like Vlora municipality, where the free-rider problem is on a smaller scale compared to larger municipalities in the world.
Relationship between positive parenting and cyberbullying perpetration among adolescents: role of self-esteem and smartphone addiction
Authors: Kim Ji Hye , Song Hye Young , Jung Gye Hyun
Published: March 2024

Introduction

Adolescents may perpetrate cyberbullying in cyberspace, which they perceive as a medium of social communication, and cyberbullying perpetration is closely related to adolescent behavior, mental health, and development. This study aimed to examine the relationship of certain factors related to cyberbullying in adolescents using the framework of Jessor’s problem behavior theory.

Methods

This cross-sectional study investigated the mediating effect of adolescent self-esteem and smartphone addiction in the relationship between positive parenting and adolescent cyberbullying perpetration. The secondary analysis used data from the 2018 Korean Children and Youth Panel Survey. The data on positive parenting, adolescent self-esteem, smartphone addiction, and perpetration of cyberbullying of 2,394 Korean adolescents (boys: 1,297, 54.2%) were analyzed. Hayes’ PROCESS macro program was used to confirm the mediating role.

Results

The results revealed that 26.5% (n = 634) of adolescents had perpetrated cyberbullying. Positive parenting did not directly relate to cyberbullying perpetration. Adolescent self-esteem and smartphone addiction played a mediating role between positive parenting and adolescent cyberbullying.

Discussion

Individual adolescent characteristics and parent/family system characteristics should be considered in policies aimed at preventing adolescent cyberbullying perpetration, preceded by the management of appropriate smartphone use. Improving young people’s self-esteem and teaching them how to use smartphones correctly can help prevent cyberbullying.
Sex differences in symptom network structure of depression, anxiety, and self-efficacy among people with diabetes: a network analysis
Authors: Wu Daoai , Shi Zhaoming , Wu Chenchen , Sun Weihua , Jin Guoxi
Published: March 2024

Aims

The present study aims to explore the relations between symptoms of depression and anxiety and self-efficacy among people with diabetes. At the same time, we also examined the sex difference between network structures.

Methods

This study recruited 413 participants with diabetes, and they completed Generalized Anxiety Disorder Scale (GAD-7), Patient Health Questionnaire (PHQ-9), and the Self-efficacy for Diabetes (SED). Symptom network analysis and network comparison test were used to construct and compare the depression-anxiety symptom network models of the female and male groups. Finally, we conducted flow diagrams to explore the symptoms directly or indirectly related to self-efficacy.

Results

The strongest edges in the depression-anxiety symptom networks are the edge between “GAD3” (Excessive worry) and “GAD4” (Trouble relaxing) and the edge between “PHQ1” (Anhedonia) and “PHQ4” (Energy) in the female and male groups, respectively. Most of the symptoms with the highest EI and bridge EI are related to worry and nervousness. Additionally, in the flow diagram of the female group, “PHQ6” (Guilt) has a high negative association with self-efficacy.

Conclusion

Females with diabetes are more vulnerable to depression and anxiety. Interventions targeting key symptoms in the network may be helpful in relieving the psychological problems among people with diabetes.
Four weeks of meditation training improves sustained attention in community-dwelling older adults: a proof-of-concept randomized controlled trial
Authors: Ford Sabrina D. , Nagamatsu Lindsay S.
Published: March 2024

Introduction: With our rapidly expanding population of older adults, identifying effective intervention strategies to improve cognitive functioning is an increasing priority. This study sought to examine whether 4 weeks of thrice-weekly meditation training can improve attention in older adults, as well as whether such benefits may extend to other domains of cognition as well as mobility.

Methods: Forty-three participants (mean age 68 years) were randomized into either the focused attention meditation group or the music listening control group (Clinicaltrials.gov ID NCT03417635). Participants completed three 20-minute guided group sessions per week for four consecutive weeks. Our primary outcome measure was behavioural performance on the Sustained Attention to Response Task (SART). Secondary and tertiary outcome measures included event-related potentials (ERPs) during the SART task, measures of executive functioning, and measures of mobility.

Results: We found that meditation training significantly improved attention, as demonstrated by improved SART accuracy and changes in N2 ERP amplitude and latency.

Discussion: These findings suggest that meditation may lead to changes in attention and underlying cognitive processing in older adults, although a full-scale definitive trial is needed. Future research on the long-term benefits with real world applications is warranted.
Video games and disability—a risk and benefit analysis
Authors: Kuo Hung Jen , Yeomans Michael , Ruiz Derek , Lin Chien-Chun
Published: March 2024

Purpose

Over the past decades, video games have become a substantial part of the entertainment industry. While ubiquitous, video game participation remains low among people with disabilities amid potential negative effects. This article analyzes the risks and benefits that video games may present to individuals with disabilities.

Methodology

In this conceptual article, we explored the literature pertaining to video games and disability. To better understand the impact of video games on individuals with disabilities, we focused on the unique features of video games through the lens of the Self-Determination Theory.

Findings

Our findings show that individuals with disabilities are most at risk from excessive video game use, leading to increased aggression, sedentary behavior, and negative impact on academic performance. Identified benefits include promoting physical rehabilitation and psychological well-being, improving cognitive abilities and emotional regulation, and utility in promoting exercises, and managing chronic pain.

Originality

This article presents a number of strategies and resources to help guide individuals with disabilities, educators, practitioners, and researchers in maximizing the benefits of video games while controlling the risks.
Are tactile function and body awareness of the foot related to motor outcomes in children with upper motor neuron lesions?
Authors: Marsico Petra , Meier Lea , van der Linden Marietta L. , Mercer Thomas H. , van Hedel Hubertus J. A.
Published: March 2024

Introduction

Somatosensory function can be reduced in children with Upper Motor Neuron (UMN) lesions. Therefore, we investigated relationships between somatosensory functions of the foot and motor outcomes in children with UMN lesions.

Method

In this cross-sectional study, we assessed the Tactile Threshold (TT) with monofilaments and body awareness with Tactile Localisation Tasks for spatial-related action (TLTaction) and structural-related perception (TLTperception) body representation at the foot sole. Furthermore, we assessed four motor outcomes: the Selective Control Assessment of the Lower Extremity (SCALE), the modified Timed Up and Go test (mTUG), the Gillette Functional Assessment Questionnaire (FAQ), and the Functional Mobility Scale (FMS). Spearman's correlations (ρ) were applied to assess relationships between the somatosensory function of the foot sole and the applied motor outcomes.

Results

Thirty-five children with UMN lesions, on average 11.7 ± 3.4 years old, participated. TLTperception correlated significantly with all lower limb motor outcomes (|ρ|=0.36–0.57; p < 0.05), but TLTaction (|ρ|=0.00–0.27; p = 0.15–0.97, and TT did not (|ρ|=0.01–0.83; p = 0.73–0.94). TLTperception correlated strongly with the Gross Motor Function Classification System (|ρ|=0.62; p = 0.001) in children with cerebral palsy (n = 24).

Discussion

Assessing structural body representation of the foot sole should be considered when addressing lower limb motor impairments, including gait, in children with upper motor neuron lesions. Our results suggest that the assessment of tactile function and spatial body representation may be less related to lower limb motor function.
There are alternatives. Models for sustainable employment structures in the German system of higher education
Authors: Kuhnt Mathias , Müßig Peter , Reitz Tilman
Published: March 2024

The German system of higher education and public research is characterized by a high rate of temporary contracts with short contract durations and a nearly complete absence of structured career options. About 82% of employees not holding a full professorship have fixed-term contracts, with an average contract period of 20 months. This is facilitated by a special fixed-term employment law (Wissenschaftszeitvertragsgesetz) which universities and other public research institutions have stretched to its limits. Against the background of a survey which we conducted in 2021 and whose results once more demonstrate the shortcomings of this system, we discuss alternative options in the form of model calculations. We propose a reform of employment structures and career paths that could improve not only personal working conditions but also ensure the quality of research and teaching in German academia. By quantitative comparison with the current employment situation, our model calculation demonstrates that plannable career decisions can be enabled at an early stage without changing budgets or teaching duties. We also show that the counter argument of a “congestion” of positions is not substantiated, and that young scholars will still have the opportunity to start a career in the reformed system, while the total number of employees can be kept nearly constant.
Unidentified fish sounds as indicators of coral reef health and comparison to other acoustic methods
Authors: Jarriel Sierra D. , Formel Nathan , Ferguson Sophie R. , Jensen Frants H. , Apprill Amy , Mooney T. Aran
Published: March 2024

The global decline of coral reefs is a major contributor to the global biodiversity crisis and requires improved monitoring at these critically important habitats. Non-invasive passive acoustic assessments may address this need, leveraging the rich variety and spatiotemporal variability of biological sounds present in coral reef environments and offering near-continuous temporal coverage. Despite this, acoustic metrics that reliably represent coral reef health are still debated, and ground-truthing of methods is limited. Here we investigated how the prevalence of low frequency biotic sounds (without species information) relates to coral reef health, providing a foundation from which one can compare assessment methods. We first quantified call rates of these low frequency sounds for three reefs exhibiting different community assemblages around St. John, U.S. Virgin Islands, by manually annotating presumed fish noises for 1 min every 30 min across 8 days for each site. Annotated days were selected at key points across lunar cycles. These call rates were then compared with traditional visual surveys, and several acoustic methods and indices commonly used in underwater soundscape research. We found that, overall, manually detected fish call rates successfully differentiated between the three reefs, capturing variation in crepuscular activity levels–a pattern consistent with previous work that highlights the importance of diel choruses. Moreover, fish vocal rates were predictors of hard coral cover, fish abundance, and fish species richness, while most acoustic indices failed to parse out fine distinctions among the three sites. Some, such as the Acoustic Complexity Index, failed to reveal any expected differences between sites or times of day, while the Bioacoustic Index could only identify the most acoustically active reef, otherwise having weak correlations to visual metrics. Of the indices tested, root-mean-squared sound pressure level and Acoustic Entropy, both calculated in the low frequency fish band (50–1,200 Hz), showed the strongest association with visual health measures. These findings present an important step toward using soundscape cues for reef health assessments. The limited generalizability of acoustic indices across different locations emphasizes the need for caution in their application. Therefore, it is crucial to improve methods utilizing fish sounds, such as automatic fish call detectors that are able to generalize well to new soundscapes.
LIBS for prospecting and Raman spectroscopy for monitoring: two feasibility studies for supporting in-situ resource utilization
Authors: Rammelkamp Kristin , Schröder Susanne , Lomax Bethany A. , Clavé Elise , Hübers Heinz-Wilhelm
Published: March 2024

Laser-induced breakdown spectroscopy (LIBS) and Raman spectroscopy are still rather new techniques for in-situ exploration of extraterrestrial planetary surfaces but have shown their suitability and great potential in several successful robotic missions already. Next to serving primary scientific applications, both methods can also be used in the context of in-situ resource utilization (ISRU) such as scouting for wanted substances and the surveillance of extraction processes. Here, we present two laboratory studies conducted in the context of ISRU with a focus on the chain from prospecting to extracting oxygen from lunar regolith. For LIBS, with optimized data processing and combined with state-of-the-art multivariate data analysis approaches, we show the potential of the technique for identifying samples with increased ilmenite content and for elemental quantification. The measurements were done using lunar regolith simulant and low pressures simulating vacuum on atmosphereless bodies such as the Moon. With Raman spectroscopy, we analyzed lunar regolith simulant samples that underwent electrochemical alteration for oxygen extraction and production of metal alloys demonstrating the potential of Raman spectroscopy for ISRU process monitoring. We also discuss the results in a broader context, evaluating the potential of both methods for other aspects of ISRU support.
Nutritional composition, phytochemicals, and antioxidant activities of Abies marocana Trab. needles
Authors: Zirari Malak , Aouji Marouane , Imtara Hamada , Hmouni Driss , Tarayrah Mahmoud , Noman Omar M. , El Mejdoub Nouredine
Published: March 2024

Introduction

Finding natural sources of bioactive compounds is turning into a cutting- edge task for the scientific community and industry alike. Abies marocana, Moroccan fir, holds great importance due to its ecological, economic, social, and cultural significance.

Material and Methods

The current study aimed to evaluate the needles of Abies marocana in terms of its nutritional and anti-nutrient content, bioactive components, and antioxidant capacity. The AOAC technique was used to determine the composition of the needles. Mineral content was analyzed by inductively coupled plasma optical emission spectrometry. Phytochemical screening of methanol extract was performed using standard procedures, and multiple assays evaluated antioxidant activity. The extract’s volatile profile was elucidated using GC-MS method.

Results and Discussion

The presence of various components in the needles was discovered through proximate analysis, including carbohydrates, crude protein, crude fiber, crude fat, ash, and moisture. Anti-nutrients such as chlorophyll, carotenoids, and vitamin A were confirmed. Needles are a rich source of mineral elements and contain significant levels of phenols, flavonoids, tannins, and phytosterols. Palmitic acid, 17-octadecynoic acid, and (Z)-18- octadec-9-enolide were the main components identified by GC-MS analysis. The methanolic extract exhibited substantial antioxidant capacity. The DPPH scavenging activity displayed a remarkable percentage inhibition. The integration of Abies marocana needles into sustainable diets can contribute to the well- being of humans and the planet, since the nutrient and bioactive compounds present have potential applications in the pharmaceutical and food industries.
Case Report: Inflamed Jacobson nerve: an uncommon cause of persisting otalgia after an acute otitis media
Authors: Camurdan Aybige , Kilgue Alexander , Scholtz Lars Uwe , Todt Ingo
Published: March 2024

Introduction

Otalgia can have multiple causes. Mostly otalgia is caused by a tubal dysfunction or an acute middle ear infection. This case describes a patient with an inflammation of the Jacobson's nerve causing severe persistent otalgia after an acute otitis media. The patients complaints completely disappeared after neurolysis of the Jacobson's nerve.

Case presentation

We describe a case of a 21-year-old female caucasian patient with acute otitis media and persistent intractable otalgia. Infection was first successfully controlled by antibiotics. But the patient reported a persistent otalgia not responding to analgetics. We performed a CT scan, which exhibited a regular aerated middle ear finding, and a diagnostic tympanoscopy to examine the middle ear structures particularly the tympanic Jacobson's nerve as a possible cause for persistent pain. The following neurolysis of Jacobson's nerve under general anaesthesia led to a resolution of otalgia.

Conclusion

An inflamed tympanic Jacobson's nerve is a rare observation and a persisting otalgia after an acute otitis media not responding to conservative treatment can be treated by a neurolysis.
Review of yeast culture concerning the interactions between gut microbiota and young ruminant animals
Authors: Liu Shixiong , Yang Lan , Zhang Yufei , Chen Hui , Li Xueqiang , Xu Zixuan , Du Rui , Li Xiao , Ma Jiabin , Liu Dacheng
Published: March 2024

Microorganisms inhabit the gastrointestinal tract of ruminants and regulate body metabolism by maintaining intestinal health. The state of gastrointestinal health is influenced not only by the macro-level factors of optimal development and the physiological structure integrity but also by the delicate equilibrium between the intestinal flora and immune status at the micro-level. Abrupt weaning in young ruminants causes incomplete development of the intestinal tract resulting in an unstable and unformed microbiota. Abrupt weaning also induced damages to the microecological homeostasis of the intestinal tract, resulting in the intestinal infections and diseases, such as diarrhea. Recently, nutritional and functional yeast culture has been researched to tackle these problems. Herein, we summarized current known interactions between intestinal microorganisms and the body of young ruminants, then we discussed the regulatory effects of using yeast culture as a feed supplement. Yeast culture is a microecological preparation that contains yeast, enriched with yeast metabolites and other nutrient-active components, including β-glucan, mannan, digestive enzymes, amino acids, minerals, vitamins, and some other unknown growth factors. It stimulates the proliferation of intestinal mucosal epithelial cells and the reproduction of intestinal microorganisms by providing special nutrient substrates to support the intestinal function. Additionally, the β-glucan and mannan effectively stimulate intestinal mucosal immunity, promote immune response, activate macrophages, and increase acid phosphatase levels, thereby improving the body’s resistance to several disease. The incorporation of yeast culture into young ruminants’ diet significantly alleviated the damage caused by weaning stress to the gastrointestinal tract which also acts an effective strategy to promote the balance of intestinal flora, development of intestinal tissue, and establishment of mucosal immune system. Our review provides a theoretical basis for the application of yeast culture in the diet of young ruminants.
Adaptive Nonsingular Fast-Reaching Terminal Sliding Mode Control Based on Observer for Aerial Robots
Authors: Yang Pu , Xuan Yan , Li Wanting
Published: March 2024

In this article, an observer-based adaptive non-singular fast-reaching terminal sliding mode control strategy is proposed to tackle the problem of actuator faults and uncertain disturbance in aerial robot systems. Firstly, a model of an aerial robot system is established through dynamic analysis. Next, an adaptive observer, combined with a fast adaptive fault estimation (FAFE) algorithm, is proposed to estimate system states and actuator failure and compensate for faults in a precise and prompt manner. In addition, a non-singular fast terminal sliding surface is defined, taking into account the fast convergence of the tracking errors in order to provide appropriate trajectory tracking results. Since the upper bounds of the disturbances caused by the manipulator of the system in practice are unknown, the control approach may benefit from the addition of an adaptive control strategy that can suppress the influence of uncertain disturbances. The Lyapunov stability theory demonstrates that tracking errors are able to converge stably and quickly. In the end, the contrast experiment is conducted to exhibit the effectiveness of the proposed control strategy. The results demonstrate quicker convergence and improved estimating accuracy.