bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Germination on Protein, γ-Aminobutyric Acid, Phenolic Acids, and Antioxidant Capacity in Wheat
Authors: Kim Mi Jeong , Kwak Han Sub , Kim Sang Sook
Published: September 2018

Germinated wheat is a food material with potential health benefits due to its high phenolic and antioxidant content, but the reason why germination increases this content is unclear. The aim of this study was to investigate the relationships between protein changes (determined by two-dimensional gel electrophoresis (2-DE)), phenolics, γ-aminobutyric acid (GABA) levels, and antioxidant capacity of wheat germinated for various periods (24, 48, 72, and 96 h) compared to control. Each phenolic acid tended to increase with increasing germination time. The GABA content was highest (39.98 mg/100 g dwb) after 96 h of germination. The total oxygen radical absorbance capacity (ORAC) was 1.97 times higher after 96 h than in ungerminated seeds. Fifteen proteins, among 82 proteins separated by 2-DE, were highly related with ORAC and were identified by peptide mass fingerprinting (PMS). The PMS revealed strong expression of granule bound starch synthase (GBSS) and glutathione S-transferase (GSTF) after 96 h of germination. Overall, the ORAC at 96 h exhibited a close relationship with the levels of phenolic acids, GABA, and proteins such as GBSS and GSTF. In conclusion, these findings add to the existing knowledge of wheat protein changes and their relationship to the antioxidant properties of germinating wheat seeds.
Identification, Recombinant Expression, and Characterization of LGH2, a Novel Antimicrobial Peptide of Lactobacillus casei HZ1
Authors: He Junfang , Luo Xuegang , Jin Duxin , Wang Yunyang , Zhang Tongcun
Published: September 2018

L. casei HZ1 was identified from Chinese traditional fermented milk, and angiotensin converting enzyme inhibitory peptide was separated from its culture in our previous work. Here, LGH2 was a novel AMP, identified from the genome of L. casei HZ1. Altogether, roughly 52.76% of LGH2 was α -helical, with the remainder in β -strand and random coil in 50% TFE solution tested by CD. The peptide was also an amphipathic and cationic molecule, which was composed of 20 amino acid residues. The similarity of the amino acid sequence between LGH2 and Temporin-RN3 was highest. Then, the peptide successfully expressed in E. coli Rossetta (DE3) pLysS using the SUMO fusion expression system and purified by chromatography technologies. The molecular weight of the peptide was 2448 Da determined by MALDI-TOF MS. Antimicrobial tests showed that the peptide has strong activities against G+ bacteria, special for S. aureus (MIC = 4 μM). The toxicity assay showed that the peptide exhibits a low hemolytic activity against sheep red blood cells. The antimicrobial mechanisms of LGH2 against pathogens were further investigated by dye leakage, CLSM, SEM, and FCM assays. We found that LGH2 can bind to the cell membrane, and destroy its integrity. These significant results indicate that LGH2 has great potential to treat the infections caused by pathogenic bacteria such as S. aureus, and it provides a new template to improve antimicrobial peptides targeting antibiotic-resistant pathogenic bacteria.
Improved Detection and Fragmentation of Disulphide-Linked Peptides
Authors: Maes Evelyne , Clerens Stefan , Dyer Jolon M. , Deb-Choudhury Santanu
Published: September 2018

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.
Atomic Scale Simulation on the Fracture Mechanism of Black Phosphorus Monolayer under Indentation
Authors: Liu Yang , Liu Yuhong , Luo Jianbin
Published: September 2018

Molecular dynamics simulations on the indentation process of freestanding and Pt(111)-supported black phosphorus (BP) monolayer were conducted to study the fracture mechanism of the membrane. For the freestanding BP monolayer, crack grows firstly along armchair direction and then zigzag direction during the indentation process. Whereas, for the Pt(111)-supported BP monolayer, crack growth shows no obvious directionality, with irregular distribution of crack tips. Further study on stress distribution shows that maximum normal stress component at elastic stage is in zigzag direction for the freestanding BP monolayer, and in vertical direction for the Pt(111)-supported BP monolayer. As BP monolayer is remarkably anisotropic for in-plane mechanical properties and homogeneous for out-of-plane mechanical properties, the difference of stress state may be a key reason for the different fracture behavior in these two cases. These findings may help to understand the failure mechanism of BP, when applied in nano-devices.
Preparation of Nickel Nanoparticles by Direct Current Arc Discharge Method and Their Catalytic Application in Hybrid Na-Air Battery
Authors: Su Fengmei , Qiu Xuechao , Liang Feng , Tanaka Manabu , Qu Tao , Yao Yaochun , Ma Wenhui , Yang Bin , Dai Yongnian , Hayashi Katsuro , Watanabe Takayuki
Published: September 2018

Nickel nanoparticles were prepared by the arc discharge method. Argon and argon/hydrogen mixtures were used as plasma gas; the evaporation of anode material chiefly resulted in the formation of different arc-anode attachments at different hydrogen concentrations. The concentration of hydrogen was fixed at 0, 30, and 50 vol% in argon arc, corresponding to diffuse, multiple, and constricted arc-anode attachments, respectively, which were observed by using a high-speed camera. The images of the cathode and anode jets were observed with a suitable band-pass filter. The relationship between the area change of the cathode/anode jet and the synchronous voltage/current waveform was studied. By investigating diverse arc-anode attachments, the effect of hydrogen concentration on the features of nickel nanoparticles were investigated, finding that 50 vol% H2 concentration has high productivity, fine crystallinity, and appropriate size distribution. The synthesized nickel nanoparticles were then used as catalysts in a hybrid sodium–air battery. Compared with commercial a silver nanoparticle catalyst and carbon black, nickel nanoparticles have better electrocatalytic performance. The promising electrocatalytic activity of nickel nanoparticles can be ascribed to their good crystallinity, effective activation sites, and Ni/NiO composite structures. Nickel nanoparticles prepared by the direct current (DC) arc discharge method have the potential to be applied as catalysts on a large scale.
Phosphorescent Modulation of Metallophilic Clusters and Recognition of Solvents through a Flexible Host-Guest Assembly: A Theoretical Investigation
Authors: Li Zhi-Feng , Yang Xiao-Ping , Li Hui-Xue , Zuo Guo-Fang
Published: September 2018

MP2 (Second order approximation of Møller–Plesset perturbation theory) and DFT/TD-DFT (Density functional theory/Time-dependent_density_functional_theory) investigations have been performed on metallophilic nanomaterials of host clusters [Au(NHC)2]+⋅⋅⋅[M(CN)2]−⋅⋅⋅[Au(NHC)2]+ (NHC = N-heterocyclic carbene, M = Au, Ag) with high phosphorescence. The phosphorescence quantum yield order of clusters in the experiments was evidenced by their order of μS1/ΔES1−T1 values ( μ S 1 : S0 → S1 transition dipole, ∆ E S 1 − T 1 : splitting energy between the lowest-lying singlet S1 and the triplet excited state T1 states). The systematic variation of the guest solvents (S1: CH3OH, S2: CH3CH2OH, S3: H2O) are employed not only to illuminate their effect on the metallophilic interaction and phosphorescence but also as the probes to investigate the recognized capacity of the hosts. The simulations revealed that the metallophilic interactions are mainly electrostatic and the guests can subtly modulate the geometries, especially metallophilic Au⋅⋅⋅M distances of the hosts through mutual hydrogen bond interactions. The phosphorescence spectra of hosts are predicted to be blue-shifted under polar solvent and the excitation from HOMO (highest occupied molecular orbital) to LUMO (lowest unoccupied molecular orbital) was found to be responsible for the 3MLCT (triplet metal-to-ligand charge transfer) characters in the hosts and host-guest complexes. The results of investigation can be introduced as the clues for the design of promising blue-emitting phosphorescent and functional materials.
Nano-MnO2 Decoration of TiO2 Microparticles to Promote Gaseous Ethanol Visible Photoremoval
Authors: Stucchi Marta , Boffito Daria C. , Pargoletti Eleonora , Cerrato Giuseppina , Bianchi Claudia L. , Cappelletti Giuseppe
Published: September 2018

TiO2-based photocatalysis under visible light is an attractive way to abate air pollutants. Moreover, developing photocatalytic materials on a large-scale requires safe and low-cost precursors. Both high-performance TiO2 nanopowders and visible-light active noble metals do not match these requirements. Here, we report the design of novel Mn-decorated micrometric TiO2 particles. Pigmentary TiO2 replaced unsafe nano-TiO2 and firmly supported MnOx particles. Mn replaced noble metals such as Au or Ag, opening the way for the development of lower cost catalysts. Varying Mn loading or pH during the impregnation affected the final activity, thus giving important information to optimize the synthesis. Photocatalytic activity screening occurred on the gas-phase degradation of ethanol as a reference molecule, both under ultraviolet (UV) (6 h) and Light Emitting Diode (LED) (24 h) irradiation. Mn-doped TiO2 reached a maximum ethanol degradation of 35% under visible light after 24 h for the sample containing 20% of Mn. Also, we found that an acidic pH increased both ethanol degradation and mineralization to CO2, while an alkaline pH drastically slowed down the reaction. A strict correlation between photocatalytic results and physico-chemical characterizations of the synthesized powders were drawn.
Measuring Children’s Sodium and Potassium Intakes in NZ: A Pilot Study
Authors: Eyles Helen , Bhana Neela , Lee Sang Eun , Grimes Carley , McLean Rachael , Nowson Caryl , Wall Clare
Published: September 2018

Low sodium and high potassium intakes in childhood protect against rises in blood pressure (BP) and risk of cardiovascular disease (CVD) later in life. Our aim was to pilot methods for collection of 24-h urine samples (gold standard) and diet recalls to assess sodium and potassium intakes and their food sources in 30 children aged 8–11 years at one New Zealand primary school. A diverse sample (n = 27) was recruited over a two-week period. All children provided a urine sample (71% complete) and interviewer-assisted 24-h diet recall (Intake24 software). Median (range) sodium intake was 2191 (1087 to 4786) mg/day (salt equivalent 5.5 g), potassium intake was 1776 (800–2981) mg/day, BP was 105 (84–129)/62 (53–89) mmHg, and sodium to potassium molar ratio was 2.0 (1.1–4.8). Frequent use of discretionary salt was uncommon. Major food sources of sodium were bread, pies and pastries, and bread and pasta-based dishes, and potassium were sauces and condiments, dairy products, and non-alcoholic beverages. Most participants provided adequate data and enjoyed taking part. A larger survey is warranted to confirm findings and inform a potential intervention(s). Small improvements to study procedures and resources should improve completeness of urine samples and quality of 24-h diet recall data.
Selenium, Selenoproteins, and Immunity
Authors: Avery Joseph , Hoffmann Peter
Published: September 2018

Selenium is an essential micronutrient that plays a crucial role in development and a wide variety of physiological processes including effect immune responses. The immune system relies on adequate dietary selenium intake and this nutrient exerts its biological effects mostly through its incorporation into selenoproteins. The selenoproteome contains 25 members in humans that exhibit a wide variety of functions. The development of high-throughput omic approaches and novel bioinformatics tools has led to new insights regarding the effects of selenium and selenoproteins in human immuno-biology. Equally important are the innovative experimental systems that have emerged to interrogate molecular mechanisms underlying those effects. This review presents a summary of the current understanding of the role of selenium and selenoproteins in regulating immune cell functions and how dysregulation of these processes may lead to inflammation or immune-related diseases.
Health and Nutrition Studies Related to Cereal Biodiversity: A Participatory Multi-Actor Literature Review Approach
Authors: Sofi Francesco , Dinu Monica , Pagliai Giuditta , Cei Leonardo , Sacchi Giovanna , Benedettelli Stefano , Stefani Gianluca , Gagliardi Edneia , Tosi Paola , Bocci Riccardo , Bussi Bettina , De Santis Giuseppe , Rodriguez y Hurtado Ismael , De Kochko Patrick , Riviere Pierre , Carrascosa-García María , Martínez Ignacio
Published: September 2018

Recently, a large and growing body of literature has investigated the health potential of different wheat species. In particular, a considerable number of studies dealing with nutritional aspects has grown up around the theme of the recovery of ancient wheat varieties (species that have remained unchanged over the last hundred years). According to several studies, indeed, ancient varieties present a healthier nutritional profile than modern ones. In the framework of the European project “CERERE, CEreal REnaissance in Rural Europe: embedding diversity in organic and low-input food systems”, this paper aimed to review recent research on the issue of health and nutritional cereal systems by adopting an innovative and participatory multi-actor approach which involved practitioners along with researchers. The participatory approach is the main innovation and peculiarity of this literature review. Nevertheless, the review highlights the many positive effects derived from eating whole and ancient grains such as a significant reduction in the risk of chronic diseases such as cancer, cardiovascular disease, and also a more favorable long-term weight management and increase in satiety. This review may be considered as a fruitful starting point that integrates research results to foster current and future healthier and sustainable practices in cereal systems.
Dietary Supplement Use among U.S. Children by Family Income, Food Security Level, and Nutrition Assistance Program Participation Status in 2011–2014
Authors: Jun Shinyoung , Cowan Alexandra E. , Tooze Janet A. , Gahche Jaime J. , Dwyer Johanna T. , Eicher-Miller Heather A. , Bhadra Anindya , Guenther Patricia M. , Potischman Nancy , Dodd Kevin W. , Bailey Regan L.
Published: September 2018

This analysis characterizes use of dietary supplements (DS) and motivations for DS use among U.S. children (≤18 years) by family income level, food security status, and federal nutrition assistance program participation using the 2011–2014 National Health and Nutrition Examination Survey data. About one-third (32%) of children used DS, mostly multivitamin-minerals (MVM; 24%). DS and MVM use were associated with higher family income and higher household food security level. DS use was lowest among children in households participating in the Supplemental Nutrition Assistance Program (SNAP; 20%) and those participating in the Special Supplemental Nutrition Assistance Program for Women, Infants, and Children (WIC; 26%) compared to both income-eligible and income-ineligible nonparticipants. Most children who used DS took only one (83%) or two (12%) products; although children in low-income families took fewer products than those in higher income families. The most common motivations for DS and MVM use were to “improve (42% or 46%)” or “maintain (34 or 38%)” health, followed by “to supplement the diet (23 or 24%)” for DS or MVM, respectively. High-income children were more likely to use DS and MVM “to supplement the diet” than middle- or low-income children. Only 18% of child DS users took DS based on a health practitioner’s recommendation. In conclusion, DS use was lower among children who were in low-income or food-insecure families, or families participating in nutrition assistance programs.
Inhibition of Lipid Peroxidation of Kiwicha (Amaranthus caudatus) Hydrolyzed Protein Using Zebrafish Larvae and Embryos
Authors: Vilcacundo Rubén , Barrio Daniel Alejandro , Piñuel Lucrecia , Boeri Patricia , Tombari Andrea , Pinto Adelita , Welbaum James , Hernández-Ledesma Blanca , Carrillo Wilman
Published: September 2018

Amaranth protein concentrate (APC) was hydrolyzed under in vitro gastrointestinal conditions. APC proteins were partially degraded by pepsin at pHs 1.2, 2.0, and 3.2. During the intestinal phase (pepsin/pancreatin enzymes at pH 7.0), no polypeptide bands were observed in the gel, suggesting the susceptibility of amaranth proteins to the action of digestive enzymes. The potent in vitro inhibition of lipid peroxidation, shown by the gastric and intestinal digests, was confirmed in the zebrafish larvae, with a 72.86% reduction in oxidation of lipids in the presence of the gastric hydrolysate at pH 2.0, compared to a 95.72% reduction in the presence of the gastrointestinal digest. APC digests were capable of reducing reactive oxygen species (ROS) production in the zebrafish embryo model with a value of fluorescence of 52.5% for the gastric hydrolysate, and 48.4% for the intestinal hydrolysate.
Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers
Authors: Baek In-Hyuk , Han Hyung-Seop , Baik Seungyun , Helms Volkhard , Kim Youngjun
Published: September 2018

Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid (CA) as a chemical pollutant. Adsorption and quartz crystal microbalance with dissipation monitoring experiments showed that the phage-functionalized MIP had a good binding affinity for CA, compared with the non-imprinted polymer and MIP. In addition, the reusability of the phage-functionalized MIP was demonstrated for at least five repeated cycles, without significant loss in the binding activity. The results indicate that the exposed amino acids of the phage, together with the polymer matrix, create functional binding cavities that provide higher affinity to acidic pharmaceutical compounds.
Nanomechanics and Nanorheology of Microgels at Interfaces
Authors: Backes Sebastian , Von Klitzing Regine
Published: September 2018

The review addresses nanomechanics and nanorheology of stimuli responsive microgels adsorbed at an interface. In order to measure the mechanical properties on a local scale, an atomic force microscope is used. The tip presents an indenter with a radius of curvature of a few 10 s of nm. Static indentation experiments and dynamic studies with an excited cantilever are presented. The effect of several internal and external parameters on the mechanical properties is reviewed. The focus is on the correlation between the swelling abilities of the gels and their mechanical properties. Several results are surprising and show that the relationship is not as simple as one might expect.
A Novel Synthetic UV-Curable Fluorinated Siloxane Resin for Low Surface Energy Coating
Authors: Zhu Chunfang , Yang Haitao , Liang Hongbo , Wang Zhengyue , Dong Jun , Xiong Lei , Zhou Jianping , Ke Junjun , Xu Xi , Xi Weixian
Published: September 2018

Low surface energy materials have attracted much attention due to their properties and various applications. In this work, we synthesized and characterized a series of ultraviolet (UV)-curable fluorinated siloxane polymers with various fluorinated acrylates—hexafluorobutyl acrylate, dodecafluoroheptyl acrylate, and trifluorooctyl methacrylate—grafted onto a hydrogen-containing poly(dimethylsiloxane) backbone. The structures of the fluorinated siloxane polymers were measured and confirmed by proton nuclear magnetic resonance and Fourier transform infrared spectroscopy. Then the polymers were used as surface modifiers of UV-curable commercial polyurethane (DR-U356) at different concentrations (1, 2, 3, 4, 5, and 10 wt %). Among three formulations of these fluorinated siloxane polymers modified with DR-U356, hydrophobic states (91°, 92°, and 98°) were obtained at low concentrations (1 wt %). The DR-U356 resin is only in the hydrophilic state at 59.41°. The fluorine and siloxane element contents were investigated by X-ray photoelectron spectroscopy and the results indicated that the fluorinated and siloxane elements were liable to migrate to the surface of resins. The results of the friction recovering assays showed that the recorded contact angles of the series of fluorinated siloxane resins were higher than the original values after the friction-annealing progressing.
Effective Formation of Well-Defined Polymeric Microfibers and Nanofibers with Exceptional Uniformity by Simple Mechanical Needle Spinning
Authors: Lee Hoik , Inoue Yuma , Kim Myungwoong , Ren Xuehong , Kim Ick Soo
Published: September 2018

The fabrication of nanofibers with a mechanical force has attracted increasing attention owing to its facile and easy fabrication. Herein, we demonstrate a novel and facile fabrication technique with the mechanical force, needle spinning, which utilizes a needle tip to draw a polymer solution to form fibrous structures. We studied the effect of the processing parameters to the nanofiber structure, namely, the pulling away speed, pulling away distances, needle size, and polymer concentration, which were systemically controlled. As the needle spinning provides an effective route to adjust those parameters, highly uniform nanofibers can be achieved. There are clear tendencies in the diameter; it was increased as the polymer concentration and needle size were increased, and was decreased as the pulling away distance and pulling away speed were increased. Needle spinning with a precise control of the processing parameter enables us to readily fabricate well-defined nanofibers, with controlled dimensions in diameter and length; plus, single nanofibers also can be easily formed. Those features cannot be realized in common spinning process such as electrospinning. Therefore, this technique will lead to further development of the use of mechanical force for nanofiber fabrication and will expand the range of nanofibers applications.
Shear-Flow Coupled Behavior of Artificial Joints with Sawtooth Asperities
Authors: Zhao Cheng , Zhang Rui , Zhang Qingzhao , Shi Zhenming , Yu Songbo
Published: September 2018

The coupling between hydraulic and mechanical processes in rock joints has significantly influenced the properties and applications of rock mass in many engineering fields. In this study, a series of regular shear tests and shear-flow coupled tests were conducted on artificial joints with sawtooth asperities. Shear deformation, strength, and seepage properties were comprehensively analyzed to reveal the influence of joint roughness, normal stress, and seepage pressure on shear-flow coupled behavior. The results indicate that the shear failure mode, which can be divided into sliding and cutting, is dominated by joint roughness and affected by the other two factors under certain conditions. The seepage process makes a negative impact on shear strength as a result of the mutual reinforcing of offsetting and softening effects. The evolution of hydraulic aperture during the shear-flow coupled tests embodies a consistent pattern of four stages: shear contraction, shear dilation, re-contraction, and stability. The permeability of joint sample is considerably enlarged with the increase of joint roughness, but decreases with the addition of normal stress.
Kinematic Calibration of a Cable-Driven Parallel Robot for 3D Printing
Authors: Qian Sen , Bao Kunlong , Zi Bin , Wang Ning
Published: September 2018

Three-dimensional (3D) printing technology has been greatly developed in the last decade and gradually applied in the construction, medical, and manufacturing industries. However, limited workspace and accuracy restrict the development of 3D printing technology. Due to the extension range and flexibility of cables, cable-driven parallel robots can be applied in challenging tasks that require motion with large reachable workspace and better flexibility. In this paper, a cable-driven parallel robot for 3D Printing is developed to obtain larger workspace rather than traditional 3D printing devices. A kinematic calibration method is proposed based on cable length residuals. On the basis of the kinematic model of the cable-driven parallel robot for 3D Printing, the mapping model is established among geometric structure errors, zero errors of the cable length, and end-effector position errors. In order to improve the efficiency of calibration measurement, an optimal scheme for measurement positions is proposed. The accuracy and efficiency of the kinematics calibration method are verified through numerical simulation. The calibration experiment based on the motion capture system indicates that the position error of end-effector is decreased to 0.6157 mm after calibration. In addition, the proposed calibration method is effective and verified for measurement positions outside optimal positions set through experiments.
The Fate of Chemical Pollutants with Soil Properties and Processes in the Climate Change Paradigm—A Review
Authors: Biswas Bhabananda , Qi Fangjie , Biswas Jayanta Kumar , Wijayawardena Ayanka , Khan Muhammad Atikul Islam , Naidu Ravi
Published: September 2018

Heavy metal(loid)s and organic contaminants are two major groups of pollutants in soils. The fate and exposure of such pollutants in soil depends on their chemical properties, speciation, and soil properties. Soil properties and processes that control the toxicological aspects of pollutants include temperature, moisture, organic matter, mineral fractions, and microbial activities. These processes are vulnerable to climate change associated with global warming, including increased incidences of extreme rainfall, extended dry periods, soil erosion, and a rise in sea level. Here we explain evidence that relates to the effects of climate change-driven soil processes on the mobility, transport, and storage of pollutants in soil. The review found that changes in climate could increase human exposure to soil contaminants mainly due to processes involving soil organic carbon (SOC), surface runoff, redox state, and microbial community. However, uncertainties remain in relation to the extent of contaminant toxicity to human health, which is linked to global change drivers.
The Role of Community-led Governance in Innovation Diffusion: The Case of RFID Waste Pricing System in the Republic of Korea
Authors: Lee Sabinne , Jung Kwangho
Published: September 2018

This study explores the role of community-led governance in innovation adoption by drawing determinants of RFID waste pricing system adoption. The Ministry of Environment of the Republic of Korea adopted an individual-incentive based RFID waste pricing system for apartment complexes in 2013 in an effort to reduce food waste. After invention and wide usage during World War Two, RFID has received lots of intention especially in public policy field. This innovative system using RFID machines and RFID tag-embedded cards showed effectiveness in waste reduction. Residents of each apartment complex decide whether and when to adopt the RFID system. Based on innovation diffusion theory, we would like to draw which determinant factors most affect RFID pricing system adoption. Although innovation diffusion is dependent on community level, many previous studies surveyed individuals and asked them about organizational adoption. We use non-perceptional data representing the characteristics of the level of apartment complex. This study, relying on ecological variables collected from 2018 apartment complexes across 25 autonomous districts located in Seoul City, explores significant factors related to the process of the RFID adoption. According to the empirical results, there were two important determinants: how much information the government provided, and how the apartment complex residents have opportunity to gather opinion and decide the RFID pricing system adoption. We emphasize the importance of community-led governance in adopting an innovative environmental system and how community-led governance contributes to sustainable innovation adoption.
A Systematic Initial Study of Civic Scientific Literacy in China: Cross-National Comparable Results from Scientific Cognition to Sustainable Literacy
Authors: Wu Sheng , Zhang Yi , Zhuang Zheng-Yun
Published: September 2018

Civic scientific literacy (CSL) is an important factor for the development of any country, and this is especially true for a country which is under development and at the same time pursues sustainability. In this article, we conduct the firstly systematic study of CSL in China based on survey data in 2013. Under the multi-dimension CSL framework and by using item response theory, we assess the CSL level in China, which can be fairly compared with that in U.S. and Europe (US-EU). The comparable survey results provided a number of implicational findings: for example, the status of CSL of China in 2013 would have ranked as middling and poor compared to the CSL results for the various US-EU countries in 1995 and 2005, respectively. Some group-based analyses were also conducted to show how people’s attitudes to the environment and socio-environmental behaviours correlate with the CSL-qualified rate in China. The empirical results provided by this study not only can serve as references for improving CSL in China or other emerging countries that also address the sustainability issues during development, but could also serve as indicators for future studies (e.g., in causational modelling).
Photofermentative Poly-3-Hydroxybutyrate Production by Rhodopseudomonas sp. S16-VOGS3 in a Novel Outdoor 70-L Photobioreactor
Authors: Carlozzi Pietro , Seggiani Maurizia , Cinelli Patrizia , Mallegni Norma , Lazzeri Andrea
Published: September 2018

In the present study, the performance of a 70 L photobioreactor, operating outdoors, was investigated using a purple bacterial strain as Rhodopseudomonas sp. S16-VOGS3 for producing poly-3-hydroxybutyrate (PHB). The novel photobioreactor was equipped with 5 rows L-shaped; the bottom of every row was placed in a stainless-steel tank containing water with controlled temperature. The photofermentation trials were carried out under fed-batch mode and under a semi-continuous regimen using lactic acid as the carbon source. The effect of the irradiance and the carbon/nitrogen ratio on the PHB accumulation was investigated, in order to evaluate the optimal bacterial growth. The results showed the feasibility of the prototype photobioreactor for the production of PHB by Rhodopseudomonas sp. S16-VOGS3 under the natural light/dark cycle. During the fed-batch growth (144 h long), the cumulative PHB increased quickly reaching a maximum value of 377 mg/L and decreased to 255 mg/L during the semi-continuous regimen (336 h long).
How to Develop the Interdisciplinary Innovation Teams Sustainably?—A Simulation Model from a Perspective of Knowledge Fission and Fusion
Authors: Cheng Xue , Zhang Qingpu
Published: September 2018

Knowledge interaction is vital in order that interdisciplinary innovation teams (IITs) develop sustainably. This paper aims to reveal the laws of knowledge interaction in IITs from a perspective of knowledge fission and fusion. Herein, the conceptions of knowledge fission to depict the team member’s divergent thinking and knowledge fusion to depict the team member’s convergent thinking based on the concept of social physics are proposed. Furthermore, the Markov process describing knowledge interaction is built. The paper uses a case study and a simulation analysis to explain the process of knowledge interaction. The results show that knowledge fission and knowledge fusion have different influences on the various stages of knowledge interaction. To conclude, the model built describes the complex phenomenon of the knowledge interaction process. It reveals the transformation rules from knowledge fission to knowledge fusion in the process of knowledge interaction in IITs. This study also provides new insight for IITs to maintain team sustainability.
Surveying and Digital Restoration of Towering Architectural Heritage in Harsh Environments: a Case Study of the Millennium Ancient Watchtower in Tibet
Authors: Chen Siliang , Yang Haozhong , Wang Shusheng , Hu Qingwu
Published: September 2018

Aiming at the problem of difficult data collection and modeling in high-rise ancient buildings with narrow interiors, a method is proposed in this paper for modeling and supporting digital restoration based on unmanned aerial vehicle oblique photogrammetry combined with three-dimensional (3D) laser scanning technology. The ancient watchtower complex in the Tibetan region of China is taken as an example. Firstly, the data is collected using an unmanned aerial vehicle and 3D laser scanner. Secondly, the two types of data are merged to generate a three-dimensional status model. Finally, by analyzing the status model and combining the similar remaining conditions, a virtual restoration scheme is proposed, and a 3D restoration model is established. The results show that virtual restoration based on 3D measurement technology can be used as a new method for the research and protection of towering ancient buildings, asrecorded by adopting targeted technology for digital documentation. It is necessary and effective to adopt a method combining unmanned aerial vehicle oblique photogrammetry and the ground 3D laser scanning technology in harsh environments. The digital model can promote the sustainable utilization of cultural heritage. It is necessary to analyze and make full use of the status model of such ancient buildings based on accurately measured data for the virtual restoration of the damaged ancient buildings. The status model of the ancient buildings can be used for display browsing and disaster recording. The restoration model can be dismantled and used to guide the repair work.
Influence of Perceptual Range on Human Perceived Restoration
Authors: Kim Moohan
Published: September 2018

In daily living environments, an individual’s different state of mind influences their spatial perception. The current study, based on Attention Restoration Theory, aimed to explore differences in the health utility of nature according to individual differences in spatial perception. It focused on Cheonggyecheon stream in Seoul, South Korea. Cognitive mapping and the Perceived Restorativeness Scale (PRS) were used to assess two groups’ different perceived spatial ranges and the restorative effect of the environment. After gathering data, two groups were defined: one describing only the internal area of the research site (composed of green materials), and the other illustrating the external area of the site, including buildings and roads. The former had higher overall PRS, Being Away, Fascination, and Compatibility scores. The latter had higher scores only on the Coherence subscale. These results illustrate that the frequency of nature visits and time spent traveling influence the two groups’ attentional restoration, which has great implications for highly stressful urban environments.
Empirical Study towards Corporate Social Responsibility Practices and Company Financial Performance. Evidence for Companies Listed on the Bucharest Stock Exchange
Authors: Simionescu Liliana Nicoleta , Dumitrescu Dalina
Published: September 2018

The present paper empirically examines the relation between corporate social responsibility (CSR) practices and company financial performance (CFP) for firms listed on the Bucharest Stock Exchange. Likewise, the paper analyzes the CSR policies adopted by the companies as CSR practices towards six types of stakeholders that influence the CFP. Using principal component analysis, we developed a CSR index and several specific indices for CSR practices. By estimating cross-sectional regression models, our study provides support for a positive link between CSR and CFP, when companies implement CSR policies regarding employees, environmental protection, and ethics as social practices. Further, empirical findings show that companies responsive to the CSR concept and those considering international standards and regulations for quality products and services in their business strategy enhance CFP.
New Concepts on Vertex and Edge Coloring of Simple Vague Graphs
Authors: Dey Arindam , Son Le Hoang , Kumar P. K. Kishore , Selvachandran Ganeshsree , Quek Shio Gai
Published: September 2018

The vague graph has found its importance as a closer approximation to real life situations. A review of the literature in this area reveals that the edge coloring problem for vague graphs has not been studied until now. Therefore, in this paper, we analyse the concept of vertex and edge coloring on simple vague graphs. Specifically, two new definitions for vague graphs related to the concept of the λ-strong-adjacent and ζ-strong-incident of vague graphs are introduced. We consider the color classes to analyze the coloring on the vertices in vague graphs. The proposed method illustrates the concept of coloring on vague graphs, using the definition of color class, which depends only on the truth membership function. Applications of the proposal in solving practical problems related to traffic flow management and the selection of advertisement spots are mainly discussed.
Introduction to the Special Issue: Applications of Internet of Things
Authors: Chen Chi-Hua , Al-Masri Eyhab , Hwang Feng-Jang , Ktoridou Despo , Lo Kuen-Rong
Published: September 2018

This editorial introduces the special issue, entitled “Applications of Internet of Things”, of Symmetry. The topics covered in this issue fall under four main parts: (I) communication techniques and applications, (II) data science techniques and applications, (III) smart transportation, and (IV) smart homes. Four papers on sensing techniques and applications are included as follows: (1) “Reliability of improved cooperative communication over wireless sensor networks”, by Chen et al.; (2) “User classification in crowdsourcing-based cooperative spectrum sensing”, by Zhai and Wang; (3) “IoT’s tiny steps towards 5G: Telco’s perspective”, by Cero et al.; and (4) “An Internet of things area coverage analyzer (ITHACA) for complex topographical scenarios”, by Parada et al. One paper on data science techniques and applications is as follows: “Internet of things: a scientometric review”, by Ruiz-Rosero et al. Two papers on smart transportation are as follows: (1) “An Internet of things approach for extracting featured data using an AIS database: an application based on the viewpoint of connected ships”, by He et al.; and (2) “The development of key technologies in applications of vessels connected to the Internet”, by Tian et al. Two papers on smart home are as follows: (1) “A novel approach based on time cluster for activity recognition of daily living in smart homes”, by Liu et al.; and (2) “IoT-based image recognition system for smart home-delivered meal services”, by Tseng et al.
Applications of Differential Form Wu’s Method to Determine Symmetries of (Partial) Differential Equations
Authors: Chaolu Temuer , Bilige Sudao
Published: September 2018

In this paper, we present an application of Wu’s method (differential characteristic set (dchar-set) algorithm) for computing the symmetry of (partial) differential equations (PDEs) that provides a direct and systematic procedure to obtain the classical and nonclassical symmetry of the differential equations. The fundamental theory and subalgorithms used in the proposed algorithm consist of a different version of the Lie criterion for the classical symmetry of PDEs and the zero decomposition algorithm of a differential polynomial (d-pol) system (DPS). The version of the Lie criterion yields determining equations (DTEs) of symmetries of differential equations, even those including a nonsolvable equation. The decomposition algorithm is used to solve the DTEs by decomposing the zero set of the DPS associated with the DTEs into a union of a series of zero sets of dchar-sets of the system, which leads to simplification of the computations.
Intrauterine Exposure to Cadmium Reduces HIF-1 DNA-Binding Ability in Rat Fetal Kidneys
Authors: Jacobo-Estrada Tania , Cardenas-Gonzalez Mariana , Santoyo-Sánchez Mitzi Paola , Thevenod Frank , Barbier Olivier
Published: September 2018

During embryonic development, some hypoxia occurs due to incipient vascularization. Under hypoxic conditions, gene expression is mainly controlled by hypoxia-inducible factor 1 (HIF-1). The activity of this transcription factor can be altered by the exposure to a variety of compounds; among them is cadmium (Cd), a nephrotoxic heavy metal capable of crossing the placenta and reaching fetal kidneys. The goal of the study was to determine Cd effects on HIF-1 on embryonic kidneys. Pregnant Wistar rats were exposed to a mist of isotonic saline solution or CdCl2 (DDel = 1.48 mg Cd/kg/day), from gestational day (GD) 8 to 20. Embryonic kidneys were obtained on GD 21 for RNA and protein extraction. Results show that Cd exposure had no effect on HIF-1α and prolyl hydroxylase 2 protein levels, but it reduced HIF-1 DNA-binding ability, which was confirmed by a decrease in vascular endothelial growth factor (VEGF) mRNA levels. In contrast, the protein levels of VEGF were not changed, which suggests the activation of additional regulatory mechanisms of VEGF protein expression to ensure proper kidney development. In conclusion, Cd exposure decreases HIF-1-binding activity, posing a risk on renal fetal development.
Enterohemorrhagic E. coli (EHEC)—Secreted Serine Protease EspP Stimulates Electrogenic Ion Transport in Human Colonoid Monolayers
Authors: Tse C. Ming , In Julie G. , Yin Jianyi , Donowitz Mark , Doucet Michele , Foulke-Abel Jennifer , Ruiz-Perez Fernando , Nataro James P. , Zachos Nicholas C. , Kaper James B. , Kovbasnjuk Olga
Published: September 2018

One of the characteristic manifestations of Shiga-toxin-producing Escherichia coli (E. coli) infection in humans, including EHEC and Enteroaggregative E. coli O104:H4, is watery diarrhea. However, neither Shiga toxin nor numerous components of the type-3 secretion system have been found to independently elicit fluid secretion. We used the adult stem-cell-derived human colonoid monolayers (HCM) to test whether EHEC-secreted extracellular serine protease P (EspP), a member of the serine protease family broadly expressed by diarrheagenic E. coli can act as an enterotoxin. We applied the Ussing chamber/voltage clamp technique to determine whether EspP stimulates electrogenic ion transport indicated by a change in short-circuit current (Isc). EspP stimulates Isc in HCM. The EspP-stimulated Isc does not require protease activity, is not cystic fibrosis transmembrane conductance regulator (CFTR)-mediated, but is partially Ca2+-dependent. EspP neutralization with a specific antibody reduces its potency in stimulating Isc. Serine Protease A, secreted by Enteroaggregative E. coli, also stimulates Isc in HCM, but this current is CFTR-dependent. In conclusion, EspP stimulates colonic CFTR-independent active ion transport and may be involved in the pathophysiology of EHEC diarrhea. Serine protease toxins from E. coli pathogens appear to serve as enterotoxins, potentially significantly contributing to watery diarrhea.
Recent Trends (2012–2016) of N, Si, and P Export from the Nemunas River Watershed: Loads, Unbalanced Stoichiometry, and Threats for Downstream Aquatic Ecosystems
Authors: Vybernaite-Lubiene Irma , Zilius Mindaugas , Saltyte-Vaisiauske Laura , Bartoli Marco
Published: September 2018

The Curonian Lagoon, the largest in Europe, suffers from nuisance cyanobacterial blooms during summer, probably triggered by unbalanced nutrient availability. However, nutrient delivery to this system was never analysed in detail. During 2012–2016, we analysed the monthly discharge, nutrient loads, and ecological stoichiometry at the closing section of the Nemunas River, the main nutrient source to the lagoon. The aim of this study was to investigate seasonal and annual variations of nitrogen (N), silica (Si), and phosphorous (P) with respect to discharge, climatic features, and historical trends. The nutrient loads varied yearly by up to 50% and their concentrations underwent strong seasonality, with summer N and Si limitation. The river discharge (16 ± 4 km3·yr−1) was lower than the historical average (21.8 km3·yr−1). Changes in agricultural practices resulted in similar N export from the river watershed compared to historical data (1986–2002), while sewage treatment plant improvements led to a ~60% decrease of P loads. This work contributes new data to the scattered available information on the most important nutrient source to the Curonian Lagoon. Further P reduction is needed to avoid unbalanced dissolved inorganic nitrogen and phosphorus (DIN:DIP~10) ecological stoichiometry in summer, which may stimulate undesired cyanobacterial blooms.
Among Mountains and Between Rivers: A Preliminary Appraisal of the Arrival, Spread, and Development of Daoism and Buddhism in the Central Hunan [Xiangzhong] Region
Authors: Robson James
Published: September 2018

Le but de cet essai est de fournir, à travers une étude de géographie locale, un aperçu de l'arrivée, du développement et de la diffusion du bouddhisme et du taoïsme dans la province du Hunan. Alors que l'auteur se proposait initialement de donner les contours de cette histoire jusqu'à nos jours, l'accent est surtout mis sur des périodes plus anciennes. Le Xiangzhong, ou « région centrale du Hunan », correspond à une aire délimitée par le fleuve Xiang à l'Est, les fleuves Zi et Yuan à l'Ouest, et le fleuve Li au Nord. Ceci étant dit, les sources discutées dans cet essai révèlent que les centres pour le développement de l'histoire du bouddhisme et du taoïsme dans le Hunan sont pour la plupart périphériques par rapport à cette région et demeurent très rares pour le cœur même de cette région (districts de Xinhua et d'Anhua par exemple). En conclusion, l'auteur estime que pour avoir une meilleure compréhension de l'histoire religieuse de la région du centre du Hunan, il est nécessaire d'aller au-delà des sources historiques classiques et des texte canoniques, en s'intéressant aux manuscrits locaux et, pour la période contemporaine, en effectuant des enquêtes ethnographiques. Dans cette perspective, les documents qui accompagnent les statuettes du Hunan, les manuels de rituel locaux et les généalogies sont tous de première importance pour écrire (et récrire) l'histoire religieuse de cette région, de la dynastie des Qing jusqu'à aujourd'hui.