bluuebunny/crossref_metadata_2025 huggingface dataset:
The Black Necrotic Lesion Enhanced Fusarium graminearum Resistance in Wheat
Authors: Zhao Lanfei , Su Peisen , Hou Bingqian , Wu Hongyan , Fan Yanhui , Li Wen , Zhao Jinxiao , Ge Wenyang , Xu Shoushen , Wu Shiwen , Ma Xin , Li Anfei , Bai Guihua , Wang Hongwei , Kong Lingrang
Published: June 2022

Fusarium head blight, mainly incited by Fusarium graminearum, is a devastating wheat disease worldwide. Diverse Fusarium head blight (FHB) resistant sources have been reported, but the resistance mechanisms of these sources remain to be investigated. FHB-resistant wheat germplasm often shows black necrotic lesions (BNLs) around the infection sites. To determine the relationship between BNL and FHB resistance, leaf tissue of a resistant wheat cultivar Sumai 3 was inoculated with four different F. graminearum isolates. Integrated metabolomic and transcriptomic analyses of the inoculated samples suggested that the phytohormone signaling, phenolamine, and flavonoid metabolic pathways played important roles in BNL formation that restricted F. graminearum extension. Exogenous application of flavonoid metabolites on wheat detached leaves revealed the possible contribution of flavonoids to BNL formation. Exogenous treatment of either salicylic acid (SA) or methyl jasmonate (MeJA) on wheat spikes significantly reduced the FHB severity. However, exogenous MeJA treatment prevented the BNL formation on the detached leaves of FHB-resistant wheat Sumai 3. SA signaling pathway influenced reactive oxygen species (ROS) burst to enhance BNL formation to reduce FHB severity. Three key genes in SA biosynthesis and signal transduction pathway, TaICS1, TaNPR1, and TaNPR3, positively regulated FHB resistance in wheat. A complex temporal interaction that contributed to wheat FHB resistance was detected between the SA and JA signaling pathways. Knowledge of BNLs extends our understanding of the molecular mechanisms of FHB resistance in wheat and will benefit the genetic improvement of wheat FHB resistance.
New Insights Into Tissue Culture Plant-Regeneration Mechanisms
Authors: Long Yun , Yang Yun , Pan Guangtang , Shen Yaou
Published: June 2022

Plant regeneration occurs when plants repair or replace damaged structures based on the totipotency and pluripotency of their cells. Tissue culture is one of the most widely used regenerative technologies. Recently, a series of breakthroughs were made in the study of plant regeneration. This review summarizes two regenerative pathways in tissue culture: somatic embryogenesis and de novo organogenesis. Furthermore, we review the environmental factors influencing plant regeneration from explant sources, basal culture medium, plant growth regulators, and light/dark treatment. Additionally, we analyse the molecular mechanisms underlying two pathways. This knowledge will promote an understanding of the fundamental principles of plant regeneration from precursor cells and lay a solid foundation for applying plant micropropagation and genetic modification.
The Millet of the Matter: Archeobotanical Evidence for Farming Strategies of Western Han Dynasty Core Area Inhabitants
Authors: Liao Jingwen , Li Ming , Allen Edward , Luo Wuhong , Sheng Pengfei
Published: June 2022

Despite decades of investigation, consensus has yet to be reached on when and where wheat replaced millet as the primary crop in the core regions of early Imperial China. Previous studies have suggested that wheat cultivation likely became widespread prior to or during the Han Dynasty (202 BC–AD 220). Here, we tested this hypothesis by applying archeobotanical tools to plant remains found in five pottery model granaries (cang) entombed in a newly excavated late Western Han burial at the Longzaocun cemetery in the Guanzhong Basin. This analysis allowed us to explore the extent of wheat expansion and agricultural strategies in the heartland of early dynastic China. Macro- and micro-botanical evidence shows that the Longzaocun residents consumed two kinds of crops: foxtail and common millet. Combining these findings with previous studies, we argue that millet-based multi-crop farming dominated the regional agricultural system during the Western Han Dynasty (202 BC-AD 8) and analyze the political and cultural motivations for the Han people’s usage on millet crops from the burial concepts and fiscal systems. Echoing previous studies, we argue that millets remained the most valuable subsistence food for inhabitants of the Loess Basins in the Han core, and that wheat was not cultivated on a large scale in this area during the Western Han Dynasty.
Genetic Diversity of Juglans mandshurica Populations in Northeast China Based on SSR Markers
Authors: Zhang Qinhui , Zhang Xinxin , Yang Yuchun , Xu Lianfeng , Feng Jian , Wang Jingyuan , Tang Yongsheng , Pei Xiaona , Zhao Xiyang
Published: June 2022

Juglans mandshurica is a native tree species in Northeast China. Due to habitat destruction and human disturbance, its population size has sharply decreased. Currently, information on molecular markers of J. mandshurica is limited and cannot meet the needs of germplasm resource evaluation and molecular marker-assisted breeding of J. mandshurica. Based on transcriptomic data from three tissues (leaves, bark, and fruit pericarp), we developed expressed sequence tag-simple sequence repeats (EST-SSRs) for J. mandshurica, and 15 polymorphic EST-SSR primers were initially selected. The average number of alleles (Na), expected heterozygosity (He), and the polymorphic information content (PIC) at different loci were 18.27, 0.670, and 0.797, respectively. Population genetic diversity analysis revealed that the average Na, He, and Shannon information indices (I) for 15 J. mandshurica populations were 6.993, 0.670, and 1.455, respectively. Among them, population Hunchun exhibited the highest genetic diversity (Na = 7.933, He = 0.723, and I = 1.617), while population Heihe exhibited the lowest genetic diversity (Na = 4.200, He = 0.605, and I = 1.158). STRUCTURE analysis, neighbor-joining method cluster analysis, and principal coordinate analysis showed that the 343 individuals of J. mandshurica from 15 populations were clustered into three categories. Category 1 (green) had 147 individuals from eight populations in Qingyuan, Caohekou, Jian, Ningan, Yongji, Baishishan, Helong, and Maoershan; category 2 (blue) had 81 individuals from three populations in Hulin, Boli, and Sanchazi; and category 3 (red) had 115 individuals from four populations in Heihe, Hunchun, Fangzheng, and Liangshui. Analysis of molecular variance (AMOVA) showed that genetic variations among and within individuals accounted for 16.22% and 21.10% of the total genetic variation, respectively, indicating that genetic variations within populations were greater than genetic variations among populations. The average genetic differentiation coefficient (Fst) and gene flow (Nm) between different populations were 0.109 and 4.063, respectively, implying moderate levels of genetic differentiation and gene flow. Based on the genetic diversity characteristics of different populations, we proposed various genetic conservation strategies for J. mandshurica.
Seed Priming With Melatonin Promotes Seed Germination and Seedling Growth of Triticale hexaploide L. Under PEG-6000 Induced Drought Stress
Authors: Guo Yuhui , Li Dongxiao , Liu Liantao , Sun Hongchun , Zhu Lingxiao , Zhang Ke , Zhao Haiming , Zhang Yongjiang , Li Anchang , Bai Zhiying , Tian Liwen , Dong Hezhong , Li Cundong
Published: June 2022

Melatonin (N-acetyl-5-methoxytryptamine, MT) can mitigate abotic stress, including drought stress on a number of crops. However, it is unclear whether and how seed priming with melatonin alleviates the effects of drought stress on seed germination and seedling growth of triticale (Triticale hexaploide L.). In this study, we investigated the effects of seed priming with MT on seed germination, protective enzyme activity, superoxide anion, and hydrogen peroxide in triticale under PEG-6000 induced drought stress. Seed priming with 20 μM MT alleviated the adverse effects of PEG-6000 induced drought stress on seed germination and seedling growth. Triticale seeds primed with 20 μM MT exhibited improved germination potential, germination rate, germ and radicle length. Specifically, MT priming increased the germination rate by 57.67% compared with unprimed seeds. Seed priming with melatonin also alleviated the adverse effects of PEG-6000 induced drought stress on triticale seedlings. MT pretreatment with 20 μM significantly increased the net photosynthetic rate, transpiration rate, stomatal conductance, plant height, leaf area, and relative chlorophyll concentration, enhanced the activities of superoxide dismutase and peroxidase, and decreased reactive oxygen species (ROS) and malonaldehyde content in the seeds (germ and radicle) and seedlings (leaf and root). Collectively, these results suggest that seed priming with melatonin promotes ROS scavenging capacity and enhances energy supply and antioxidant enzyme activities to alleviate the adverse effects of drought stress in triticale.
Cyclocarya paliurus Reprograms the Flavonoid Biosynthesis Pathway Against Colletotrichum fructicola
Authors: Zheng Xiang-Rong , Zhang Mao-Jiao , Qiao Yu-Hang , Li Ran , Alkan Noam , Chen Jie-Yin , Chen Feng-Mao
Published: June 2022

Cyclocarya paliurus is an endemic Chinese tree species with considerable medicinal, timber, and horticultural value. The anthracnose disease of C. paliurus is caused by the fungal pathogen Colletotrichum fructicola, which results in great losses in yield and quality. Here, resistance evaluation of six cultivars of C. paliurus exhibited varying degrees of resistance to C. fructicola infection, where Wufeng was the most resistant and Jinggangshan was the most susceptive. Physiological measurements and histochemical staining assays showed that the Wufeng cultivar exhibits intense reactive oxygen species accumulation and defense capabilities. A multiomics approach using RNA sequencing and metabolome analyses showed that resistance in C. paliurus (Wufeng) is related to early induction of reprogramming of the flavonoid biosynthesis pathway. In vitro antifungal assays revealed that the flavonoid extracts from resistant cultivars strongly inhibited C. fructicola hyphal growth than susceptible cultivars. Relative gene expression analysis further demonstrated the pivotal antifungal role of C. paliurus flavonoids in targeting Colletotrichum appressorium formation. Together, these results represent a novel resistance mechanism of C. paliurus against anthracnose through the reprogramming of flavonoids, which will lay a foundation for breeding anthracnose-resistant varieties and the application of flavonoid extraction of C. paliurus as a natural antifungal treatment.
eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7
Authors: Liu Taibo , Liu Qianyu , Yu Zhen , Wang Chunling , Mai Huafu , Liu Guolan , Li Ruijing , Pang Gang , Chen Dingwu , Liu Huili , Yang Jiangyi , Tao Li-Zhen
Published: June 2022

Eukaryotic translation initiation factor 4E1 (eIF4E1) is required for the initiation of protein synthesis. The biological function of eIF4E1 in plant–potyvirus interactions has been extensively studied. However, the role of eIF4E1 in Arabidopsis development remains unclear. In this study, we show that eIF4E1 is highly expressed in the embryo and root apical meristem. In addition, eIF4E1 expression is induced by auxin. eIF4E1 mutants show embryonic cell division defects and short primary roots, a result of reduced cell divisions. Furthermore, our results show that mutation in eIF4E1 severely reduces the accumulation of PIN-FORMED (PIN) proteins and decreases auxin-responsive gene expression at the root tip. Yeast two-hybrid assays identified that eIF4E1 interacts with an RAC/ROP GTPase activator, RopGEF7, which has been previously reported to be involved in the maintenance of the root apical meristem. The interaction between eIF4E1 and RopGEF7 is confirmed by protein pull-down and bimolecular fluorescent complementation assays in plant cells. Taken together, our results demonstrated that eIF4E1 is important for auxin-regulated embryo development and root growth. The eIF4E1–RopGEF7 interaction suggests that eIF4E1 may act through ROP signaling to regulate auxin transport, thus regulating auxin-dependent patterning.
Feeling Blue and Getting Red: An Exploratory Study on the Effect of Color in the Processing of Emotion Information
Authors: Kang June , Park Yeo Eun , Yoon Ho-Kyoung
Published: June 2022

Specific emotions and colors are associated. The current study tested whether the interference of colors with affective processing occurs solely in the semantic stage or extends to a more complex stage like the lexical processing of emotional words. We performed two experiments to determine the effect of colors on affective processing. In Experiment 1, participants completed a color-emotion priming task. The priming stimulus included a color-tinted (blue, red, and gray) image of a neutral face, followed by a target stimulus of gray-scaled emotional (angry and sad) and neutral faces after 50 ms. Experiment 2 used a modified emostroop paradigm and superimposed emotion words on the center of the color-tinted emotional and neutral faces. Results showed the priming effect of red for the angry face compared to the control, but not in blue for the sad face compared to the control. However, responses to the blue-sad pair were significantly faster than the red-sad pair. In the color-emostroop task, we observed a significant interaction between color and emotion target words in the modified emostroop task. Participants detected sad targets more accurately and faster in blue than red, but only in the incongruent condition. The results indicate that the influence of color in the processing of emotional information exists at the semantic level but found no evidence supporting the lexical level effect.
Influence of Film Role on the Positive Development of College Students’ Entrepreneurial Behavior Using Positive Psychology
Authors: Zhang Hang , Long Huizhen , Chen Yinchang
Published: June 2022

To help College Students improve their entrepreneurial psychology and enrich their innovative achievements, this paper investigates College Students’ entrepreneurial behavior based on the theory of positive psychology under the background of multiculturalism. Firstly, the background and development status of multiculturalism are expounded, and the main features of the multicultural era are pointed out; Secondly, the current situation is explained for College Students’ mental health and entrepreneurial behavior, and the specific connotation of College Students’ entrepreneurial psychology is summarized according to entrepreneurial behavior, including entrepreneurial consciousness, entrepreneurial will, entrepreneurial capability, and entrepreneurial personality; Thirdly, the positive effects of inspirational films and roles on the psychology of College Students are discussed. From the connotation of film and television culture, the main features of films are reviewed: intuitiveness, immediacy, universality, entertainment, and orientation. Finally, according to the psychological impact of inspirational films on College Students, a Questionnaire Survey (QS) is designed and distributed among the local College Students, and assumptions are put forward. The results show that the differences of College Students’ entrepreneurial level are mainly reflected in gender, urban, and rural household registration, only-child-or-no, and entrepreneurial experience, and the gender differences are mainly in entrepreneurial consciousness, will, and personality (p < 0.05); There are significant differences in consciousness, will, capability, and personality between urban and rural household registration and entrepreneurial experience (p < 0.05); The differences between the only-child and non-only-child are obvious in three aspects: will, capability, and personality (p < 0.05). After the intervention of inspirational films, the average scores of all dimensions of the entrepreneurial level of students in the intervention group are 35.45, 36.41, 38.17, and 37.22, respectively, which have a certain improvement compared with the situation before the intervention. There are significant differences in the four dimensions (p < 0.05), which is statistically significant, indicating that the intervention of inspirational films has a positive impact on students’ entrepreneurial level.
Red Tape and Community Workers’ Proactive Behavior During COVID-19: Applying the Job Demands–Resources Model
Authors: Hu Wei , Zhang Shengjie , Liu Songbo
Published: June 2022

Since the outbreak of COVID-19, community workers’ proactive behavior has played a noteworthy role in the crisis response. Previous research has not highlighted this group and their proactive behavior. To address this important gap, drawing upon the job demands–resources (JD–R) model, this study explores how red tape affects proactive behavior and investigates the mediating role of lack of goal progress (LOGP) and the moderating role of public service motivation (PSM) in this relationship. Based on a two-wave survey with a sample of 656 community workers in China, we found a negative relationship between red tape and proactive behavior. Moreover, this study showed that LOGP mediated this relationship. Contrary to our hypothesis, PSM did not moderate the relationship between LOGP and proactive behavior. These findings have important theoretical and practical implications and can better inform community work during COVID-19.
The Evolution of Food Calls: Vocal Behaviour of Sooty Mangabeys in the Presence of Food
Authors: Quintero Fredy , Touitou Sonia , Magris Martina , Zuberbühler Klaus
Published: June 2022

The two main theories of food-associated calls in animals propose functions either in cooperative recruitment or competitive spacing. However, not all social animals produce food calls and it is largely unclear under what circumstances this call type evolves. Sooty mangabeys (Cercocebus atys) do not have food calls, but they frequently produce grunts during foraging, their most common vocalisation. We found that grunt rates were significantly higher when subjects were foraging in the group’s periphery and with small audiences, in line with the cooperative recruitment hypothesis. In a subsequent field experiment we presented highly desired food items and found that discovering individuals called, unless harassed by competitors, but that the calls never attracted others, confirming that the grunts do not convey any information referential to food. Our data thus suggest that the evolution of cooperative food calling is a two-step process, starting with increased motivation to vocalise in the feeding context, followed by the evolution of acoustic variants derived from context-general contact calls. This evolutionary transition may only occur in species that feed on clumped, high-quality resources where social feeding is competitive, a condition not met in sooty mangabeys.
Institutional Pressures and Servitization Paradox: The Moderating Effect of Organizational Identity Orientations
Authors: Wang Hui , Lu Xiaojing , Hu Chaping , Wang Haijun
Published: June 2022

It is believed in many studies that the servitization of manufactures is driven by internal economy, but the situation in China may be somewhat different. In this study, we consider the influence of external institutional environment on manufacturers’ servitization and the final performance, and discuss the moderating effect of organizational identity orientation on firms’ response to external institutional pressures. We conduct a survey where we collect responses from 312 manufacturers in China. Regression analyses are performed to test whether institutional pressures (normative pressure, mimetic pressure, and coercive pressure) coming from the external institutional environment have a positive effect on the level of manufacturing servitization or not. Moreover, we study if this positive effect is moderated by the individualistic identity orientation and the relational identity orientation. We also examine the impact of servitization strategy on manufacture’s market performance and financial performances. Furthermore, we separate out the influences comes from institutional pressures by using a new estimated method and try to explain the cause of “servitization paradox.” Our study is innovative in that it bridges the servitization and institutional theory, and provides practical guidance for the adoption of manufacturing servitization strategy.
Brief Remote Intervention to Manage Food Cravings and Emotions During the COVID-19 Pandemic: A Pilot Study
Authors: Devonport Tracey J. , Chen-Wilson Chao-Hwa , Nicholls Wendy , Robazza Claudio , Cagas Jonathan Y. , Fernández-Montalvo Javier , Choi Youngjun , Ruiz Montse C.
Published: June 2022

As a result of the COVID-19 pandemic people have endured potentially stressful challenges which have influenced behaviors such as eating. This pilot study examined the effectiveness of two brief interventions aimed to help individuals deal with food cravings and associated emotional experiences. Participants were 165 individuals residing in United Kingdom, Finland, Philippines, Spain, Italy, Brazil, North America, South Korea, and China. The study was implemented remotely, thus without any contact with researchers, and involved two groups. Group one participants were requested to use daily diaries for seven consecutive days to assess the frequency of experience of their food cravings, frequency of giving in to cravings, and difficulty resisting cravings, as well as emotional states associated with their cravings. In addition to completing daily food diaries, participants in group two were asked to engage in mindful eating practice and forming implementation intentions. Participants assessed their perceived changes in eating, wellbeing, and health at the beginning and end of the intervention. Repeated measures MANOVAs indicated that participants experienced significantly less food cravings (i.e., craving experience, acting on cravings, difficulty resisting), as well as lower intensities of unpleasant states associated with cravings across time (T1 vs. T7). In contrast to our hypothesis, the main effects of the group (food craving diary vs. food craving diary and mindful eating practice) were not significant. Participants reported less eating and enhanced wellbeing at the end of the study (T7 vs. T1). Our findings can be used to inform future remote interventions to manage food cravings and associated emotions and highlight the need for alternative solutions to increase participant engagement.
Learning English as a Foreign Language Writing Skills in Collaborative Settings: A Cognitive Load Perspective
Authors: Jiang Dayu , Kalyuga Slava
Published: June 2022

Learning to write in a foreign language is a complex cognitive process. The process-genre approach is a common instructional practice adopted by language teachers to develop learners’ writing abilities. However, the interacting elements of procedural knowledge, linguistic knowledge, and generic knowledge in this approach may exceed the capacity of an individual learner’s working memory, thus actually hindering the acquisition of writing skills. According to the collective working memory effect, it was hypothesized that teaching writing skills of English as a foreign language by adopting a process-genre approach in collaborative conditions could lead to better writing performance, lower cognitive load, and higher instructional efficiency. The reported experiment compared learning writing skills of English as a foreign language in individual and collaborative instructional conditions from a cognitive load perspective, a rarely adopted approach in this field. The results indicated that the collaborative instructional condition was more effective and efficient than the individual instructional condition in improving the quality of written products as well as in optimizing the cognitive (working memory) load experienced by the learners. Measures of cognitive load were used to support the cognitive load theory’s interpretation of the results, which is the unique contribution of this research study to the field.
The Association Between Morningness-Eveningness Preference, Depression, Anxiety and Insomnia Among Chinese Textile Workers With or Without Shift Work
Authors: Jiang Jiaqi , Wang Dongfang , Scherffius Andrew , Chen Dingxuan , Ma Zijuan , Chen Zihao , Zhang Yifan , Yu Qian , Fan Fang
Published: June 2022

Objective

Circadian preference and mental health disorders are closely related to insomnia. This study aimed to evaluate insomnia symptoms in textile factory workers with different work schedules, and to investigate the association between insomnia, morningness–eveningness preference, anxiety, and depression.

Methods

A total of 3,883 textile workers were assessed using the 3-items of Pittsburg Sleep Quality Index, Composite Scale of Morningness, Beck Anxiety Inventory, Center for Epidemiologic Studies-Depression Scale, and socio-demographic questionnaires.

Results

The prevalence rate of insomnia in textile workers was 16.7% (N = 646), with 49.8% (N = 322) were shift workers. Among shift workers (N = 1,833), 9.5% had difficulty initiating sleep, and almost 9.0% suffered from early morning awakening, a rate significantly higher than among daytime workers. Logistics regressions revealed that work schedule was insignificantly associated with insomnia. Depression (OR = 1.034, 95% CI = 1.022–1.046) and anxiety (OR = 1.031, 95% CI = 1.018–1.043) positively predicted insomnia, whereas morningness preference (OR = 0.977, 95% CI = 0.960–0.995) decreased the likelihood of insomnia. Furthermore, mediation analysis showed that both anxiety and depression independently mediated the association between circadian preferences and insomnia in textile workers with or without shift work.

Conclusion

This study highlighted the insomnia, depression, and anxiety of textile factory workers in a Chinese textile factory. To improve insomnia symptoms, interventions to promote morningness circadian preference and reduce depressive and anxious symptoms among workers are encouraged.
Effects of Stressors of COVID-19 on Chinese College Students' Problematic Social Media Use: A Mediated Moderation Model
Authors: Zhao Jun , Ye Baojuan , Yu Li , Xia Fei
Published: June 2022

Purpose

Isolation policies are long-term and strictly enforced in China during the COVID-19 outbreak. Social media might be widely used for communication, work, understanding the development of the epidemic, etc. However, these behaviors might lead to problematic social media use. The present study investigated the effect of stressors of COVID-19 on problematic social media use, as well as the internal mechanisms involved.

Methods

One thousand three hundred seventy-three Chinese college students (Mage = 19.53, SDage = 1.09) were recruited randomly from four grades who completed Coronavirus Stress Scale, Fear of Missing Out Scale, Problematic Mobile Social Media Usage Assessment Questionnaire, and Regulatory Emotional Self-Efficacy Scale.

Results

Stressors of COVID-19 were positively related to problematic social media use. The link between stressors of COVID-19 and problematic social media use was mediated by fear of missing out. Additionally, the association between fear of missing out and problematic social media use, as well as the association between stressors of COVID-19 and problematic social media use were moderated by regulatory emotional self-efficacy.

Conclusion

The current findings reveal the mechanism that may be used to reduce the likelihood of problematic social media use in the context of the COVID-19 outbreak. To prevent and intervene in problematic social media use during the COVID-19 pandemic, this study stressed the importance of decreasing the fear of missing out and enhancing regulatory emotional self-efficacy.
Effectiveness and functional magnetic resonance imaging outcomes of Tuina therapy in patients with post-stroke depression: A randomized controlled trial
Authors: Tao Jiming , Zhang Shuaipan , Kong Lingjun , Zhu Qingguang , Yao Chongjie , Guo Qingjuan , Wu Jiajia , Shan Chunlei , Fang Min
Published: June 2022

Objective

To investigate the effectiveness and functional magnetic resonance imaging (fMRI) outcomes of Tuina therapy in patients with post-stroke depression (PSD).

Methods

This was a single-center, randomized, two-armed, controlled trial. Eighty-four patients with PSD were selected and randomly assigned to a Tuina therapy group or a routine rehabilitation control group. The patients underwent five 20-min treatment sessions per week over a period of 2 weeks. The primary outcome measure was change in Hamilton Depression Rating Scale (HAMD) score over the 2 weeks of intervention, whereas the secondary outcome measures were changes in Fugl-Meyer Assessment (FMA) score, Modified Barthel index (MBI), and Mini Mental State Examination (MMSE) score.

Results

The Tuina group showed significantly improved HAMD scores compared to the routine rehabilitation control group (5.85, [2.54, 9.16]). For the secondary outcomes, the Tuina group showed better MMSE scores than the routine rehabilitation group (1.97, [1.19, 2.76]); however, there were no significant differences between the other secondary outcomes of both groups (P > 0.05). After 2 weeks, both groups showed a significant decrease in HAMD score compared to baseline. In addition, the Tuina group showed a significant decrease in MMSE score compared to baseline (2.35, [1.8, 2.9]); however, there were no significant differences in the MBI and FMA scores of the two group after the intervention (P > 0.05). Regarding fMRI results, the zALFF values of the right caudate nucleus, right putamen, right insula, left superior temporal gyrus, right parahippocampal gyrus, right hippocampus, left middle temporal gyrus, left angular gyrus, and left thalamus were higher in the Tuina group. In the Tuina group, the functional connectivity between the hippocampus and thalamus, and the thalamus and caudate nucleus, were significantly different (P <0.01). In addition, the zALFF value of the hippocampus was significantly negatively correlated with HAMD score. No serious adverse events were observed in both groups.

Conclusion

Tuina therapy administered 10 times within 2 weeks is safe and can effectively relieve depression and improve cognitive function in patients with PSD. This finding may be closely related to the effect of Tuina therapy on the activation and functional connectivity of the hippocampus.

Clinical Trial Registration

http://www.chictr.org.cn/showproj.aspx?proj=55151, identifier ChiCTR200003388.
Gender Gap in Scientific Publications on COVID-19 in Italy During the First Wave of the Pandemic: An Observational Study
Authors: Mazzalai Elena , Turatto Federica , De Vito Corrado
Published: June 2022

Background

Worldwide, concerns rise on how COVID-19 pandemic impacted heavily on women, even on those belonging to the scientific community. The Italian scientific production regarding the COVID-19 throughout the first months of the health emergency could help to understand the heft of female researchers in this unique period.

Objectives

This study aims to investigate the gender gap in the scientific production on COVID-19 in Italy during the first months of the pandemic.

Methods

A systematic search of the literature was conducted and, for each included study, first and last author's gender, type of study, number of co-authors, type of affiliation, journal's Impact Factor (IF) and specialization were extracted. Descriptive and univariate analyses were performed.

Results

22.2% of the articles were signed by a woman as first author, 18.1% as last authors. Female authorship was less frequent than male authorship regardless of the type of study, number of co-authors, type of affiliation and field of specialization.

Conclusion

This analysis reveal a low prevalence of studies with a female first or last author and suggests that the low share of female authors publishing on COVID-19 during the considered timespan is a transversal issue throughout the Italian medical field.
Research Progress of Ferroptosis Regulatory Network and Bone Remodeling in Osteoporosis
Authors: Yan Chunlu , Zhang Jinlong , An Fangyu , Wang Jiayu , Shi Yao , Yuan Lingqing , Lv Donghui , Zhao Yanzhen , Wang Yongfeng
Published: June 2022

Ferroptosis was induced the programmed cell death with iron overload Fenton reaction. Currently, ferroptosis has not been studied thoroughly. Existing studies have confirmed that ferroptosis involves the metabolisms of the Fe, lipids, amino acid, each mechanism is mutually independent but interrelated, and they are formed a complex regulatory network. Other evidence supports that ferroptosis is participated osteoporotic bone remodeling, predominantly affecting the interaction between bone formation and bone resorption, explicitly bone resorption exceeded bone formation. Based on previous studies, this review will summarize the regulatory network mechanism of ferroptosis on bone remodeling and reveal the role of ferroptosis in osteoporosis (OP).
Analysis on Four Derivative Waveforms of Photoplethysmogram (PPG) for Fiducial Point Detection
Authors: Suboh Mohd Zubir , Jaafar Rosmina , Nayan Nazrul Anuar , Harun Noor Hasmiza , Mohamad Mohd Shawal Faizal
Published: June 2022

Fiducial points of photoplethysmogram (PPG), first derivative PPG (VPG), and second derivative PPG (APG) are essential in extracting numerous parameters to diagnose cardiovascular disease. However, the fiducial points were usually detected using complex mathematical algorithms. Inflection points from derivatives waveforms are not thoroughly studied, whereas they can significantly assist in peak detection. This study is performed to investigate the derivative waveforms of PPG and use them to detect the important peaks of PPG, VPG, and APG. PPGs with different morphologies from 43 ischemic heart disease subjects are analyzed. Inflection points of the derivative waveforms up to the fourth level are observed, and consistent information (derivative markers) is used to detect the fiducial points of PPG, VPG, and APG with proper sequence. Moving average filter and simple thresholding techniques are applied to detect the primary points in VPG and the third derivative waveform. A total of twelve out of twenty derivative markers are found reliable in detecting fiducial points of two common types of PPG. Systolic peaks are accurately detected with 99.64% sensitivity and 99.38% positive predictivity using the 43 IHD dataset and Complex System Laboratory (CSL) Pulse Oximetry Artifact Labels database. The study has introduced the fourth derivative PPG waveform with four main points, which are significantly valuable for detecting the fiducial points of PPG, VPG, and APG.
Multi-Omics Analysis and Machine Learning Prediction Model for Pregnancy Outcomes After Intracytoplasmic Sperm Injection–in vitro Fertilization
Authors: Chen Fangying , Chen Yixin , Mai Qinyun
Published: June 2022

Background

To explore the methylation profiles in cumulus cells (CCs) of women undergoing intracytoplasmic sperm injection–in vitro fertilization (ICSI–IVF) and establish a prediction model of pregnancy outcomes using machine learning approaches.

Methods

Methylation data were retrieved from the Gene Expression Omnibus (GEO) database, and differentially methylated genes (DMGs) were subjected to gene set analyses. Support vector machine (SVM), random forest (RF), and logistic regression (LR) were used to establish the prediction model, and microarray data from GEO was analyzed to identify differentially expressed genes (DEGs) associated with the dichotomous outcomes of clinical pregnancy (pregnant vs. non-pregnant). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis provided multi-dimensional validation for selected DMGs.

Results

A total of 338 differentially methylated CpG sites associated with 146 unique genes across the genome were identified. Among the identified pathways, the prominent ones were involved in the regulation of cell growth and oocyte development (hsa04340, hsa04012, hsa04914, hsa04614, hsa04913, hsa04020, and hsa00510). The area under the curve (AUC) of machine learning classifiers was 0.94 (SVM) vs. 0.88 (RF) vs. 0.97 (LR). 196 DEGs were found in transcriptional microarray. Mapped genes were selected through overlapping enriched pathways in transcriptional profiles and methylated data of CCs, predictive of successful pregnancy.

Conclusions

Methylated profiles of CCs were significantly different between women receiving ICSI-IVF procedures that conceived successfully and those that did not conceive. Machine learning approaches are powerful tools that may provide crucial information for prognostic assessment. Pathway analysis may be another way in multiomics analysis of cumulus cells.
The Potential Contribution of Dental Foci and Oral Mucositis to Febrile Neutropenia in Patients Treated With Myelosuppressive Chemotherapy for Solid Tumors and Lymphoma
Authors: Zecha Judith A. E. M. , Raber-Durlacher Judith E. , Laheij Alexa M. G. A. , Westermann Anneke M. , de Lange Jan , Smeele Ludi E.
Published: June 2022

Introduction

Febrile neutropenia (FN) is a potential life-threatening complication of myelosuppressive chemotherapy, particularly when induced by infection. There is evidence that FN can originate from the oral cavity, but its contribution to FN is largely understudied in patients treated for solid tumors. The aim of this study was to assess the prevalence of FN in these patients and to evaluate its relation with dental foci and oral mucositis.

Material and Methods

A prospective longitudinal observational study was conducted. Patients diagnosed with solid tumors and lymphoma scheduled to be treated with myelosuppressive chemotherapy with an intermediate risk of developing FN were included. A pre-chemotherapy dental examination was performed and patients were followed during and after chemotherapy regimen. During subsequent hospital visits for chemotherapy administration, the oral cavity was inspected and oral mucositis (OM) was scored using the CTC-AE version 3.0. When patients presented with fever, a comprehensive full body examination including laboratory/microbiological/imaging investigation was performed.

Results

Eighty-eight patients were included. Pre-chemotherapy, 39 patients (44.3%) were diagnosed with a dental focus. During chemotherapy, 46 patients developed OM (53.4%), of which 15 patients had a maximum score of grade II (ulcerative mucositis). Ten patients developed FN during the follow-up period. Patients with FN more often suffered from ulcerative OM compared to patients without FN; both FN and mucositis risk was associated with the myelotoxicity of chemotherapy. However, no relation could be established between the presence of dental foci prior to chemotherapy and the development of FN (p > 0.05).

Conclusion

A significant relation was identified between ulcerative OM and FN, but no robust conclusions could be drawn with respect to a relationship between the presence of dental foci and FN.
Techno-Economic Optimization of a Low-Temperature Organic Rankine System Driven by Multiple Heat Sources
Authors: Dokl Monika , Kravanja Zdravko , Čuček Lidija
Published: June 2022

This study presents the optimization of organic Rankine cycle (ORC) which utilizes low temperature waste heat from the aluminum production process and two low temperature renewable energy sources, solar thermal energy and geothermal energy. As geothermal energy is present at lower temperature level compared to the other two heat sources, two separate ORC cycles are considered. Optimization of the proposed system is performed based on a non-linear programming (NLP) formulation by maximizing the thermodynamic and economic performance of the system. The main variables considered in the model include temperature, pressure, flowrate, mass enthalpy, and energy flows of all the streams in the system. To optimize the variables in the system, correlations were developed, which were formulated as NLP models and optimized by minimizing the sum of least squares. The results show that most of the generated power output can be provided by the waste heat, while the lowest by the solar energy due to the relatively low average solar irradiance at considered location. When monthly time periods are considered, the highest electricity production is generated by the working fluid R1245fa in June, and amounts to 830.4 kW from waste heat, 246.5 kW from geothermal energy and 149.4 kW from solar energy. The proposed system is economically feasible for all three studied working fluids and the discount rates of 2% and higher. The final conclusions indicate that the proposed ORC system utilizing waste heat, geothermal and solar thermal energy, can generate power in a more sustainable way.
A Strong Correlation Between the Severity of Flatfoot and Symptoms of Knee Osteoarthritis in 95 Patients
Authors: Zhang Min , Nie Mao-dan , Qi Xin-zheng , Ke Song , Li Jun-wei , Shui Yang-yang , Zhang Zhuo-yue , Wang Min , Cheng Cheng-Kung
Published: June 2022

Objective

The purpose of this study is to assess the association between the presence and severity of flatfoot and symptoms of knee OA.

Methods

95 participants with knee OA were recruited from a patient cohort at a regional hospital. Symptoms of knee OA, including knee degeneration, femorotibial alignment, pain, stiffness and dysfunction were assessed using the Kellgren-Lawrence (K-L) grading system, femoral-tibial angle (FTA), and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Participants were divided into groups with flatfoot (mild, moderate and severe) and without flatfoot based on the Clarke's angle. Linear regression and ordinal logistic regression were used for statistical analysis, as appropriate.

Results

Having flatfoot was associated with a significantly increased risk of having a higher K-L grade (OR: 20.03; 95% CI, 5.88, 68.27; p < 0.001), smaller FTA (Beta: −2.96; 95% CI, −4.41, −1.50; p < 0.001), higher pain score (Beta: 0.47; 95% CI, 0.24, 0.69; p < 0.001) and greater loss of function (Beta: 0.25; 95% CI, 0.02, 0.48; p = 0.03). Severe grades of flat feet were associated with a higher K-L grade (OR: 0.19; 95% CI, 0.08, 0.44; p < 0.001), smaller FTA (Beta: 1.51; 95% CI, 0.66, 2.35; p = 0.001), higher pain score (Beta: −0.25; 95% CI, −0.39, −0.11; p = 0.001), greater stiffness (Beta: −0.24; 95% CI, −0.38, −0.09; p = 0.002) and greater loss of function (Beta: −0.27; 95% CI, −0.41, −0.14; p < 0.001).

Conclusion

The results indicated that the severity of flattening is significantly associated with symptoms of knee OA. For the conservative management of knee OA, both flatfoot and its severity should be carefully considered.
Mycotoxin Contamination of Feeds and Raw Materials in China in Year 2021
Authors: Hao Wei , Li Anping , Wang Jinyong , An Gang , Guan Shu
Published: June 2022

In this research, we performed a large-scale survey of mycotoxin contamination in several feed commodities and assessed regional differences in mycotoxin occurrence in maize across China in 2021. Concentrations of aflatoxins, zearalenone (ZEN), fumonisins, and trichothecenes type B were analyzed in 2,643 raw material and compound feed samples collected from eight provinces. Generally, trichothecenes type B, fumonisins, and ZEN were most prevalent and detected in averages of positive concentrations at 1,167, 1,623, and 204 μg/kg, respectively. In the new season maize, samples were also seriously infested with trichothecenes type B, fumonisins, and ZEN, and their averages of positive concentrations were 1,302, 2,518, and 225 μg/kg, respectively. Wheat was commonly contaminated with trichothecenes type B and ZEN, and the highest concentration levels of trichothecenes type B, fumonisins, and ZEN were all detected in the samples from maize by-products. Among the different geographical regions, distinct trends were observed in new season maize. Samples from Shandong province were highly contaminated with trichothecenes type B, fumonisins, and ZEN, while special attention should be paid to aflatoxins and fumonisins in Anhui and Jiangsu provinces in East China. In addition, the present survey showed that compound feeds and raw materials are commonly contaminated by multiple mycotoxins. Trichothecenes type B and ZEN concentrations were correlated significantly in this survey.
On Edge Pruning of Communication Networks under an Age-of-Information Framework
Authors: Sawwan Abdalaziz , Wu Jie
Published: June 2022

Effective non-repetitive routing among nodes in a network is an essential function in communication networks. To achieve that, pruning the links of the network is helpful with the trade-off of making the network less robust in transmitting messages while reducing redundancy to increase flow with limited network bandwidth, so we enhance the quality of service (QoS). In our paper, we study the case that if a link removal has either no effect or an insignificant effect on the Age of Information (AoI) of the messages delivered in the communication network. The pruning of such links can be applied to the k least significant links in terms of their impact on the AoI of the messages transmitted in the system. The objective of our work is to study the effect of pruning a number of links in a network on the AoI, in order to reduce the redundancy of the messages that may be received at the destination many times while transmitted only once. In our context, the objective of the communication system would be to maintain the information from the source as fresh as possible when it arrives at the destination while reducing the redundancy of messages. In this work, we introduce an efficient reduction method designed for series-parallel networks with links of exponentially distributed wait times. In addition, we consider the deterministic case and present the pruning technique when link removal would not affect the AoI. Lastly, we present a comprehensive simulation to study the effect of pruning the links on the AoI of the network and the redundancy of messages received by the destination.
Retrofitting Cost Modeling in Aircraft Design
Authors: Della Vecchia Pierluigi , Mandorino Massimo , Cusati Vincenzo , Nicolosi Fabrizio
Published: June 2022

Aircraft retrofitting is a challenging task involving multiple scenarios and stakeholders. Providing a strategy to retrofit an existing platform needs detailed knowledge of multiple aspects, ranging from aircraft performance and emissions, development and conversion costs to the projected operating costs. This paper proposes a methodology to account for retrofitting costs at an industrial level, explaining the activities related to such a process. Costs are mainly derived from three contributions: development costs, conversion costs and equipment acquisition costs. Different retrofitting packages, such as engine conversion and onboard systems electrification, are applied in the retrofitting of an existing 90 PAX regional turbofan aircraft, highlighting the impact on both aircraft performance and industrial costs. Multiple variables and scenarios are considered regarding trade-offs and decision-making, including the number of aircraft to be retrofitted, the heritage of an aircraft and its utilization, the fuel price and the airport charges. The results show that a reduction of 15% in fuel demand and emissions are achievable, considering a fleet of 500 platforms, through a conspicuous investment of around EUR 20 million per aircraft (50% of the estimated price). Furthermore, depending on the scenarios driven by the regulatory authorities, governments or airlines, this paper provides a useful methodology to evaluate the feasibility of retrofitting activities.