bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Steel Fiber Types and Volume Fraction on the Physical and Mechanical Properties of Concrete
Authors: Wang Zijian , Li Hao , Zhang Xin , Chang Yongzai , Wang Yi , Wu Liming , Fan Hanxiu
Published: May 2023

Different types and amounts of steel fibers have varying effects on the improvement of concrete’s mechanical properties. In order to identify the most suitable steel fiber types for the practical production of prefabricated pavements and derive a formula to predict and evaluate the mechanical properties based on steel fiber volume fraction, this study conducted experimental research on the physical mechanical properties of concrete using the method of equal volume substitution of coarse aggregate. The influence of steel fiber type and volume fraction on the microstructure and failure mechanism of steel fiber reinforced concrete (SFRC) was analyzed through electron microscopy scanning. The mechanical properties of plain concrete were used as benchmark. The results showed that when the steel fiber volume fractions were 0.6% and 1.5%, the bending and split tensile strengths of milled steel fibers were 3.1% higher than those of hooked-end steel fibers and the compressive strength of SFRC was significantly increased by 13.5%. The comprehensive mechanical properties of wave-shaped steel fibers were inferior to the first two types. Considering the requirements of SFRC in engineering, milled steel fibers are more suitable to be concrete components. This is because the tangling property of the hooked-end steel fibers makes them difficult to be shaped. In contrast, the smooth surface of milled steel fibers exerts a smaller impact on the forming of specimen and they are more economical. The mechanical properties of SFRC improve as the volume fraction of steel fibers increases from 0% to 2%, but start to decline when the volume fraction exceeds 2%. A volume fraction of 0.6% for steel fibers is sufficient to meet the standard for bending and tensile strength in heavy-duty concrete pavements. Finally, the relationship expressions between the compressive strength, flexural strength, and split tensile strength of SFRC and the steel fiber volume fraction were obtained through fitting the experimental data using Origin software.
Examining and Reforming the Rothermel Surface Fire Spread Model under No-Wind and Zero-Slope Conditions for the Karst Ecosystems
Authors: Zhang Yunlin , Tian Lingling
Published: May 2023

The Rothermel model, which has been widely used to predict the rate of forest fire spread, has errors that restrict its ability to reflect the actual rate of spread (ROS). In this study, the fuels from seven typical tree species in the Karst ecosystems in southern China were considered as the research objects. Through indoor burning simulation, three methods, namely directly using the Rothermel model, re-estimating the parameters of the Rothermel, and reforming the model, were evaluated for applicability in Karst ecosystems. We found that the direct use of the Rothermel model for predicting the ROS in the Karst ecosystems is not practical, and the relative error can be as high as 50%. However, no significant differences between the prediction effect of re-estimating the parameters of the Rothermel and the reformed model were found, but the reform model showed more evident advantages of being simpler, and the errors were lower. Our research proposes a new method that is more suitable for predicting the rate of forest fire spread of typical fuels in Karst ecosystems under flat and windless conditions, which is of great significance for further understanding and calculating the ROS of forest fires in the region.
Can Wood Pellets from Canada’s Boreal Forest Reduce Net Greenhouse Gas Emissions from Energy Generation in the UK?
Authors: Ter-Mikaelian Michael T. , Chen Jiaxin , Desjardins Sabrina M. , Colombo Stephen J.
Published: May 2023

We present the results of a study on the climate forcing effects of replacing coal for power generation in the United Kingdom (UK) with wood pellets produced in northern Ontario, Canada. Continuous wood pellet production from two biomass sources were considered: fiber from increased harvesting of standing live trees (stemwood scenario) and from harvest residue provided by ongoing harvesting operations (residue scenario). In both scenarios, biomass was collected from harvesting operations in two forest management units (FMUs) with contrasting harvest residue treatments: natural decay of slash piles in the Hearst FMU and slash pile burning in the Kenora FMU. Life cycle emissions associated with wood pellets were assessed for production, transportation, and combustion to replace coal at a hypothetical power generating station in the UK. Greenhouse gas (GHG) emissions and removals in wood pellet and coal scenarios were assessed using two methods: global warming potential (GWP)-based mass balance and dynamic life cycle assessment (LCA) approaches. In the stemwood scenario, climate change mitigation from replacing coal with wood pellets was not achieved within the study timeline (2020–2100). In the residue scenario, immediate climate change mitigation was achieved with fiber sourced from the Kenora FMU where the current practice is to burn slash piles; for the Hearst FMU, where slash is allowed to decompose in the forest, climate change mitigation occurred 11.6 and 3.1 years after biomass collection began, as assessed by the mass balance and dynamic LCA methods, respectively. Factors affecting mitigation potential in the studied scenarios are discussed.
Thinning Increases Individual Tree Growth While Reducing the Growth Heterogeneity of Lodgepole Pine
Authors: Ara Mostarin , Pinno Bradley D. , Scaria Francis , Froese Robert E. , Bokalo Mike
Published: May 2023

The positive effect of thinning on individual tree growth is well known, but the subsequent growth dynamics of individual trees over a rotation is relatively unknown, even though this is critical for interpreting actual thinning effects. Therefore, in this study, we aimed to investigate the thinning response of individual tree growth dynamics of lodgepole pine (Pinus contorta var. latifolia) over rotation using an individual tree growth model. We used data from an operational site to use as input to the Mixedwood Growth Model, an individual tree growth model, to simulate tree growth throughout the rotation. Overall, we found that thinning increased the absolute growth of individual trees and reduced the growth heterogeneity throughout the rotation. Combining pre-commercial thinning prior to commercial thinning provided maximum growth and less growth variability in individual trees. The positive effect of thinning was immediate and declined with time since thinning with most of the responses occurred within the first 10–15 years of thinning.
Strategic Information Suppression in Borrowing and Pre-Lending Cognition: Theory and Evidence
Authors: Chen Zhongwen , Zhao Xiaojian
Published: May 2023

This paper theoretically studies the interaction between an informed borrower and an uninformed lender facing possible default of a loan application. The lender is motivated to invest cognitive resources before making a lending decision. If the regulatory fine is weak, it is impossible for a bad-debt borrower to fully disclose his situation in the application. In this case, when the likelihood of a bad debt is low, the borrower always claims that nothing in the application is wrong. Otherwise, the borrower randomizes between full disclosure and information suppression. The transaction cost of the lender’s pre-lending cognition increases with the default probability, as the default probability is small and decreases thereafter. Evidence from a peer-to-peer lending platform with 816,274 observations between 2012 and 2015 in the United States is largely consistent with our model implications.
Transcriptional Dysregulations of Seven Non-Differentially Expressed Genes as Biomarkers of Metastatic Colon Cancer
Authors: Lv Xiaoying , Li Xue , Chen Shihong , Zhang Gongyou , Li Kewei , Wang Yueying , Duan Meiyu , Zhou Fengfeng , Liu Hongmei
Published: May 2023

Background: Colon cancer (CC) is common, and the mortality rate greatly increases as the disease progresses to the metastatic stage. Early detection of metastatic colon cancer (mCC) is crucial for reducing the mortality rate. Most previous studies have focused on the top-ranked differentially expressed transcriptomic biomarkers between mCC and primary CC while ignoring non-differentially expressed genes. Results: This study proposed that the complicated inter-feature correlations could be quantitatively formulated as a complementary transcriptomic view. We used a regression model to formulate the correlation between the expression levels of a messenger RNA (mRNA) and its regulatory transcription factors (TFs). The change between the predicted and real expression levels of a query mRNA was defined as the mqTrans value in the given sample, reflecting transcription regulatory changes compared with the model-training samples. A dark biomarker in mCC is defined as an mRNA gene that is non-differentially expressed in mCC but demonstrates mqTrans values significantly associated with mCC. This study detected seven dark biomarkers using 805 samples from three independent datasets. Evidence from the literature supports the role of some of these dark biomarkers. Conclusions: This study presented a complementary high-dimensional analysis procedure for transcriptome-based biomarker investigations with a case study on mCC.
Factors Associated with the Level of Knowledge about Biosafety against COVID-19 in Peruvian Dental Students: A Cross-Sectional Study under a Multivariable Regression Model
Authors: Santome-Pariona John , Briceño-Vergel Gissela , Córdova-Limaylla Nancy , Ladera-Castañeda Marysela , Huamani-Echaccaya José , Tolmos-Valdivia Rita , Huamani-Cantoral Juan , Solís-Dante Fredy , Cervantes-Ganoza Luis , Cayo-Rojas César
Published: May 2023

Aim: Biosafety is a set of preventive measures aimed at controlling risk factors arising from biological, physical, and/or chemical agents. This topic is particularly important in the dental field since saliva is the main biological agent of the transmission of coronavirus. The present study aimed to determine the factors associated with the level of knowledge about biosafety against COVID-19 in Peruvian dentistry students. Materials and Methods: The present observational, cross-sectional, and analytical study evaluated 312 Peruvian dentistry students. A validated 20-question questionnaire was used to measure the level of knowledge. The nonparametric Mann–Whitney U and Kruskal–Wallis tests were used to compare levels of knowledge between categories of each variable. A logit model was used to evaluate associated factors such as sex, age, marital status, place of origin, academic year of study, being in the academic upper third, history of COVID-19, and living with vulnerable family members. A significance level of p < 0.05 was considered. Results: 36.2%, 31.4%, and 32.4% presented poor, fair, and good knowledge levels, respectively. Students under 25 years of age were 64% less likely to pass the biosafety against COVID-19 questionnaire than students 25 years of age and older (OR = 0.36; CI: 0.20–0.66). Students in the academic upper third were nine times more likely to pass the test than other students (OR = 9.38; CI: 4.61–19.07). Finally, third-year students were 52% less likely to pass the exam than fifth-year students (OR = 0.48; CI: 0.28–0.83). Conclusion: Only a minority of dentistry students had a good level of knowledge about biosafety against COVID-19. Younger and less educated students were more likely to fail the questionnaire. On the other hand, those students with outstanding academic performance were more likely to pass the questionnaire.
Abscisic Acid: A Potential Secreted Effector Synthesized by Phytophagous Insects for Host-Plant Manipulation
Authors: Seng Stephannie , Ponce Gabriela E. , Andreas Peter , Kisiala Anna , De Clerck-Floate Rosemarie , Miller Donald G. , Chen Ming-Shun , Price Peter W. , Tooker John F. , Emery R. J. Neil , Connor Edward F.
Published: May 2023

Abscisic acid (ABA) is an isoprenoid-derived plant signaling molecule involved in a wide variety of plant processes, including facets of growth and development as well as responses to abiotic and biotic stress. ABA had previously been reported in a wide variety of animals, including insects and humans. We used high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-(ESI)-MS/MS) to examine concentrations of ABA in 17 species of phytophagous insects, including gall- and non-gall-inducing species from all insect orders with species known to induce plant galls: Thysanoptera, Hemiptera, Lepidoptera, Coleoptera, Diptera, and Hymenoptera. We found ABA in insect species in all six orders, in both gall-inducing and non-gall-inducing species, with no tendency for gall-inducing insects to have higher concentrations. The concentrations of ABA in insects often markedly exceeded those typically found in plants, suggesting it is highly improbable that insects obtain all their ABA from their host plant via consumption and sequestration. As a follow-up, we used immunohistochemistry to determine that ABA localizes to the salivary glands in the larvae of the gall-inducing Eurosta solidaginis (Diptera: Tephritidae). The high concentrations of ABA, combined with its localization to salivary glands, suggest that insects are synthesizing and secreting ABA to manipulate their host plants. The pervasiveness of ABA among both gall- and non-gall-inducing insects and our current knowledge of the role of ABA in plant processes suggest that insects are using ABA to manipulate source-sink mechanisms of nutrient allocation or to suppress host-plant defenses. ABA joins the triumvirate of phytohormones, along with cytokinins (CKs) and indole-3-acetic acid (IAA), that are abundant, widespread, and localized to glandular organs in insects and used to manipulate host plants.
Comparative Study of Porous Iron Foams for Biodegradable Implants: Structural Analysis and In Vitro Assessment
Authors: Gąsior Gabriela , Grodzicka Marlena , Jędrzejewski Tomasz , Wiśniewski Marek , Radtke Aleksandra
Published: May 2023

Biodegradable metal systems are the future of modern implantology. This publication describes the preparation of porous iron-based materials using a simple, affordable replica method on a polymeric template. We obtained two iron-based materials with different pore sizes for potential application in cardiac surgery implants. The materials were compared in terms of their corrosion rate (using immersion and electrochemical methods) and their cytotoxic activity (indirect test on three cell lines: mouse L929 fibroblasts, human aortic smooth muscle cells (HAMSC), and human umbilical vein endothelial cells (HUVEC)). Our research proved that the material being too porous might have a toxic effect on cell lines due to rapid corrosion.
Social Media as the Accelerator of Neoliberal Subjectivation and an Entrepreneurial Influencer Self among Youth?—The Hungarian Theater
Authors: Iványi Márton
Published: May 2023

Arguably, social media provides a new playground for the ever-expanding processes of neoliberal subjectivation in accordance with social or ethical standards such as the principles of performance and pleasure, competitiveness and consumerism vis à vis the general population. According to relevant data analyzed in the context of this research, any corresponding model anticipating such tendencies may seem to have limited validity in the context of Hungary, applying to certain segments of the population (aged 18–35) using social networking sites, but not necessarily a general experience. Thus, the present paper offers a theoretical and a Hungarian youth-focused empirical framework based on contemporary experiences for understanding the domestic nuances of real or perceived processes of neoliberal subjectivation.
Production of Open-Cell Metal Foams by Recycling of Aluminum Alloy Chips
Authors: Jozić Sonja , Lela Branimir , Krolo Jure , Jakovljević Suzana
Published: May 2023

In this paper, an innovative sustainable method of producing metal foams was presented. The base material was aluminum alloy waste in the form of chips obtained by machining process. The leachable agent, used to create pores in the metal foams, was natrium chloride, which was later removed by leaching, resulting in metal foams with open cells. Open-cell metal foams were produced with three different input parameters: volume percentage of natrium chloride, compaction temperature, and force. The obtained samples were subjected to compression tests during which displacements and compression forces were measured to obtain the necessary data for further analysis. To determine the influence of the input factors on the selected response values such as relative density, stress and energy absorption at 50% deformation, an analysis of variance was performed. As expected, the volume percentage of natrium chloride was shown to be the most influential input factor because it has a direct impact on the obtained metal foam porosity and thus on the density. The optimal values of the input parameters with which the metal foams will have the “most desirable” performances are a 61.44% volume percentage of natrium chloride, a compaction temperature of 300 °C and a compaction force of 495 kN.
Enhancing the Mechanical Properties of Cu–Al–Ni Shape Memory Alloys Locally Reinforced by Alumina through the Powder Bed Fusion Process
Authors: Abolhasani Daniyal , Kwon Ha-Neul , Park Yong-Han , Moon Young-Hoon
Published: May 2023

A classical problem with Cu-based shape memory alloys (SMAs) is brittle fracture at triple junctions. This alloy possesses a martensite structure at room temperature and usually comprises elongated variants. Previous studies have shown that introducing reinforcement into the matrix can refine grains and break martensite variants. Grain refinement diminishes brittle fracture at triple junctions, whereas breaking the martensite variants can negatively affect the shape memory effect (SME), owing to martensite stabilization. Furthermore, the additive element may coarsen the grains under certain circumstances if the material has a lower thermal conductivity than the matrix, even when a small amount is distributed in the composite. Powder bed fusion is a favorable approach that allows the creation of intricate structures. In this study, Cu–Al–Ni SMA samples were locally reinforced with alumina (Al2O3), which has excellent biocompatibility and inherent hardness. The reinforcement layer was composed of 0.3 and 0.9 wt% Al2O3 mixed with a Cu–Al–Ni matrix, deposited around the neutral plane within the built parts. Two different thicknesses of the deposited layers were investigated, revealing that the failure mode during compression was strongly influenced by the thickness and reinforcement content. The optimized failure mode led to an increase in fracture strain, and therefore, a better SME of the sample, which was locally reinforced by 0.3 wt% alumina under a thicker reinforcement layer.
A Review on Sustainable Inks for Printed Electronics: Materials for Conductive, Dielectric and Piezoelectric Sustainable Inks
Authors: Sanchez-Duenas Leire , Gomez Estibaliz , Larrañaga Mikel , Blanco Miren , Goitandia Amaia M. , Aranzabe Estibaliz , Vilas-Vilela José Luis
Published: May 2023

In the last decades, the demand for electronics and, therefore, electronic waste, has increased. To reduce this electronic waste and the impact of this sector on the environment, it is necessary to develop biodegradable systems using naturally produced materials with low impact on the environment or systems that can degrade in a certain period. One way to manufacture these types of systems is by using printed electronics because the inks and the substrates used are sustainable. Printed electronics involve different methods of deposition, such as screen printing or inkjet printing. Depending on the method of deposition selected, the developed inks should have different properties, such as viscosity or solid content. To produce sustainable inks, it is necessary to ensure that most of the materials used in the formulation are biobased, biodegradable, or not considered critical raw materials. In this review, different inks for inkjet printing or screen printing that are considered sustainable, and the materials that can be used to formulate them, are collected. Printed electronics need inks with different functionalities, which can be mainly classified into three groups: conductive, dielectric, or piezoelectric inks. Materials need to be selected depending on the ink’s final purpose. For example, functional materials such as carbon or biobased silver should be used to secure the conductivity of an ink, a material with dielectric properties could be used to develop a dielectric ink, or materials that present piezoelectric properties could be mixed with different binders to develop a piezoelectric ink. A good combination of all the components selected must be achieved to ensure the proper features of each ink.
Flexible Pressure Sensors Based on Microcrack Structure and Composite Conductive Mechanism for Medical Robotic Applications
Authors: Zou Qiang , Xie Yuheng , Yin Yunjiang , Liu Baoguo , Yu Yi
Published: May 2023

With the advancement of intelligent medical robot technology, machine touch utilizing flexible sensors has emerged as a prominent research area. In this study, a flexible resistive pressure sensor was designed incorporating a microcrack structure with air pores and a composite conductive mechanism of silver/carbon. The aim was to achieve enhanced stability and sensitivity with the inclusion of macro through-holes (1–3 mm) to expand the sensitive range. This technology solution was specifically applied to the machine touch system of the B-ultrasound robot. Through meticulous experimentation, it was determined that the optimal approach involved uniformly blending ecoflex and nano carbon powder at a mass ratio of 5:1, and subsequently combining the mixture with an ethanol solution of silver nanowires (AgNWs) at a mass ratio of 6:1. This combination of components resulted in the fabrication of a pressure sensor with optimal performance. Under the pressure testing condition of 5 kPa, a comparison of the resistance change rate was conducted among samples using the optimal formulation from the three processes. It was evident that the sample of ecoflex-C-AgNWs/ethanol solution exhibited the highest sensitivity. Its sensitivity was increased by 19.5% compared to the sample (ecoflex-C) and by 11.3% compared to the sample (ecoflex-C-ethanol). The sample (ecoflex-C-AgNWs/ethanol solution), which only incorporated internal air pore microcracks without through-holes, exhibited sensitive response to pressures below 5 N. However, with the addition of through-holes, the measurement range of its sensitive response increased to 20 N, representing a 400% increase in the measurement range.
Methods in Nutrition & Gut Microbiome Research: An American Society for Nutrition Satellite Session [13 October 2022]
Authors: Hughes Riley L. , Frankenfeld Cara L. , Gohl Daryl M. , Huttenhower Curtis , Jackson Scott A. , Vandeputte Doris , Vogtmann Emily , Comstock Sarah S. , Kable Mary E.
Published: May 2023

The microbial cells colonizing the human body form an ecosystem that is integral to the regulation and maintenance of human health. Elucidation of specific associations between the human microbiome and health outcomes is facilitating the development of microbiome-targeted recommendations and treatments (e.g., fecal microbiota transplant; pre-, pro-, and post-biotics) to help prevent and treat disease. However, the potential of such recommendations and treatments to improve human health has yet to be fully realized. Technological advances have led to the development and proliferation of a wide range of tools and methods to collect, store, sequence, and analyze microbiome samples. However, differences in methodology at each step in these analytic processes can lead to variability in results due to the unique biases and limitations of each component. This technical variability hampers the detection and validation of associations with small to medium effect sizes. Therefore, the American Society for Nutrition (ASN) Nutritional Microbiology Group Engaging Members (GEM), sponsored by the Institute for the Advancement of Food and Nutrition Sciences (IAFNS), hosted a satellite session on methods in nutrition and gut microbiome research to review currently available methods for microbiome research, best practices, as well as tools and standards to aid in comparability of methods and results. This manuscript summarizes the topics and research discussed at the session. Consideration of the guidelines and principles reviewed in this session will increase the accuracy, precision, and comparability of microbiome research and ultimately the understanding of the associations between the human microbiome and health.
Antiplasmodial, Trypanocidal, and Genotoxicity In Vitro Assessment of New Hybrid α,α-Difluorophenylacetamide-statin Derivatives
Authors: Araujo-Lima Carlos Fernando , de Cassia Castro Carvalho Rita , Rosario Sandra Loureiro , Leite Debora Inacio , Aguiar Anna Caroline Campos , de Souza Santos Lizandra Vitoria , de Araujo Julianna Siciliano , Salomão Kelly , Kaiser Carlos Roland , Krettli Antoniana Ursine , Bastos Monica Macedo , Aiub Claudia Alessandra Fortes , de Nazaré Correia Soeiro Maria , Boechat Nubia , Felzenszwalb Israel
Published: May 2023

Background: Statins present a plethora of pleiotropic effects including anti-inflammatory and antimicrobial responses. A,α-difluorophenylacetamides, analogs of diclofenac, are potent pre-clinical anti-inflammatory non-steroidal drugs. Molecular hybridization based on the combination of pharmacophoric moieties has emerged as a strategy for the development of new candidates aiming to obtain multitarget ligands. Methods: Considering the anti-inflammatory activity of phenylacetamides and the potential microbicidal action of statins against obligate intracellular parasites, the objective of this work was to synthesize eight new hybrid compounds of α,α-difluorophenylacetamides with the moiety of statins and assess their phenotypic activity against in vitro models of Plasmodium falciparum and Trypanosoma cruzi infection besides exploring their genotoxicity safety profile. Results: None of the sodium salt compounds presented antiparasitic activity and two acetated compounds displayed mild anti-P. falciparum effect. Against T. cruzi, the acetate halogenated hybrids showed moderate effect against both parasite forms relevant for human infection. Despite the considerable trypanosomicidal activity, the brominated compound revealed a genotoxic profile impairing future in vivo testing. Conclusions: However, the chlorinated derivative was the most promising compound with chemical and biological profitable characteristics, without presenting genotoxicity in vitro, being eligible for further in vivo experiments.
Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome
Authors: Nikolic Marina , Lazarevic Nevena , Novakovic Jovana , Jeremic Nevena , Jakovljevic Vladimir , Zivkovic Vladimir , Bradic Jovana , Pecarski Danijela , Tel-Çayan Gülsen , Glamocija Jasmina , Sokovic Marina , Gregori Andrej , Petrovic Jovana
Published: May 2023

The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.
Pharmacokinetic and Biomarker Quantification Studies on Vancomycin-Loaded PEGylated Liposomes and Its Potential to Reduce Vancomycin-Induced Kidney Injury: A Rat Study
Authors: Joshi Medha D. , Iacoban Paulina , Scheetz Marc H.
Published: May 2023

Vancomycin is a commonly used antibiotic in hospital settings, especially against Methicillin-resistant staphylococcus aureus (MRSA). One of the major adverse events of vancomycin use in adults is kidney injury. The drug concentration, specifically the area under the concentration curve, predicts kidney injury in adults receiving vancomycin. To attempt to reduce vancomycin-induced nephrotoxicity, we have successfully encapsulated vancomycin in polyethylene glycol-coated liposomes (PEG-VANCO-lipo). We have previously carried out in vitro cytotoxicity studies on kidney cells using PEG-VANCO-lipo and found it to be minimally toxic compared to the standard vancomycin. In this study, we have dosed male adult rats with PEG-VANCO-lipo or vancomycin HCl and compared plasma vancomycin concentrations and KIM-1 as an injury biomarker in rat urine. Male Sprague Dawley rats (350 ± 10 g) were administered vancomycin (n = 6) or PEG-VANCO-lipo (n = 6) 150 mg/kg/day for three days using an IV infusion in the left jugular vein catheter. Blood was collected for plasma at 15, 30, 60, 120, 240, and 1440 min after the first and the last IV dose. Urine was collected 0–2, 2–4, 4–8, and 8–24 h after the first and the last IV infusions using metabolic cages. The animals were observed for three days after the last compound administration. Vancomycin was quantified in plasma by LC-MS/MS. Urinary KIM-1 analysis was done by using an ELISA kit. Three days after the last dose, under terminal anesthesia with IP ketamine (65–100 mg/kg) and xylazine (7–10 mg/kg), rats were euthanized. Vancomycin urine and kidney concentrations and KIM-1 were lower on day three in the PEG-Vanco-lipo group compared to the vancomycin group (p < 0.05, ANOVA and/or t-test). There was a significant reduction in plasma vancomycin concentration on day one and day three (p < 0.05, t-test) in the vancomycin group compared to the PEG-VANCO-lipo group. Vancomycin-loaded PEGylated liposomes resulted in lower levels of kidney injury, as noted by a decrease in KIM-1 values. Moreover, longer circulation in plasma with increased concentration in plasma as opposed to the kidney was observed with the PEG-VANCO-lipo group. The results indicate the high potential of PEG-VANCO-lipo in decreasing the nephrotoxicity of vancomycin clinically.
Nafcillin-Loaded Photocrosslinkable Nanocomposite Hydrogels for Biomedical Applications
Authors: Toader Gabriela , Podaru Ionela , Rusen Edina , Diacon Aurel , Ginghina Raluca , Alexandru Mioara , Zorila Florina , Gavrila Ana , Trica Bogdan , Rotariu Traian , Ionita Mariana
Published: May 2023

Skin infections are frequently treated via intravenous or oral administration of antibiotics, which can lead to serious adverse effects and may sometimes contribute to the proliferation of resistant bacterial strains. Skin represents a convenient pathway for delivering therapeutic compounds, ensured by the high number of blood vessels and amount of lymphatic fluids in the cutaneous tissues, which are systematically connected to the rest of the body. This study provides a novel, straightforward method to obtain nafcillin-loaded photocrosslinkable nanocomposite hydrogels and demonstrates their performance as drug carriers and antimicrobial efficacy against Gram-positive bacteria. The novel formulations obtained, based on polyvinylpyrrolidone, tri(ethylene glycol) divinyl ether crosslinker, hydrophilic bentonite nanoclay, and/or two types of photoactive (TiO2 and ZnO) nanofillers, were characterized using various analytical methods (transmission electron microscopy (TEM), scanning electron microscopy–energy-dispersive X-ray analysis (SEM-EDX), mechanical tests (tension, compression, and shear), ultraviolet-visible spectroscopy (UV-Vis), swelling investigations, and via specific microbiological assays (“agar disc diffusion method” and “time-kill test”). The results reveal that the nanocomposite hydrogel possessed high mechanical resistance, good swelling abilities, and good antimicrobial activity, demonstrating a decrease in the bacteria growth between 3log10 and 2log10 after one hour of direct contact with S. aureus.
1064/1319 nm Dual-Wavelength Alternating Electro-Optic Q-Switched Laser Based on the Common Q-Switching Bias Voltage
Authors: Sun Jingdong , Yu Chunhe , Dong Yuan , Wu Chunting , Jin Guangyong
Published: May 2023

A dual-wavelength alternating electro-optic (EO) Q-switched laser operating at 1064 and 1319 nm is designed, which takes the structure of double the gain crystals and a single EO modulator with the common Q-switching bias voltage (CQBV). The output characteristics of alternating dual-wavelength pulse lasers are studied via simulations and experiments. The results show that the energy ratio of the two lasing wavelengths can be controlled by changing the CQBV. This is because the CQBV affects the loss of two resonators, 1064 and 1319 nm, at the same time. The gain–loss relationship in the dual-wavelength laser resonators can be controlled by changing the CQBV in a certain range.
Biological Safety Assessments of High-Purified Ovine Collagen Type I Biomatrix for Future Therapeutic Product: International Organisation for Standardisation (ISO) and Good Laboratory Practice (GLP) Settings
Authors: Fadilah Nur Izzah Md , Ahmat Nazeha , Hao Looi Qi , Maarof Manira , Rajab Nor Fadilah , Idrus Ruszymah Binti Hj , Fauzi Mh Busra
Published: May 2023

Wound care management is incredibly challenging for chronic injuries, despite the availability of various types of wound care products in the market. However, most current wound-healing products do not attempt to mimic the extracellular matrix (ECM) and simply provide a barrier function or wound covering. Collagen is a natural polymer that involves a major constituent of the ECM protein, thus making it attractive to be used in skin tissue regeneration during wound healing. This study aimed to validate the biological safety assessments of ovine tendon collagen type-I (OTC-I) in the accredited laboratory under ISO and GLP settings. It is important to ensure that the biomatrix will not stimulate the immune system to produce any adverse reaction. Therefore, we successfully extracted collagen type-I from the ovine tendon (OTC- I) using a method of low-concentration acetic acid. The three-dimensional (3D) skin patch of spongy OTC-I was a soft and white colour, being tested for safety and biocompatibility evaluations based on ISO 10993-5, ISO 10993-10, ISO 10993-11, ISO 10993-23, USP 40 <151>, and OECD 471. For the dermal sensitisation and acute irritation test, none of the tested animals displayed any erythema or oedema effects (p > 0.005). In addition, there were no abnormalities detected in the organ of the mice after being exposed to OTC-I; additionally, no morbidity and mortality were observed in the acute systemic test under the guideline of ISO 10993-11:2017. The grade 0 (non-reactive) based on ISO 10993-5:2009 was graded for the OTC-I at 100% concentration and the mean number of the revertant colonies did not exceed 2-fold of the 0.9% w/v sodium chloride compared to the tester strains of S. typhimurium (TA100, TA1535, TA98, TA1537), and E. coli (WP2 trp uvrA). Our study revealed that OTC-I biomatrix does not present any adverse effects or abnormalities in the present study’s condition of induced skin sensitization effect, mutagenic and cytotoxic towards cells and animals. This biocompatibility assessment demonstrated a good agreement between in vitro and in vivo results regarding the absence of skin irritation and sensitization potential. Therefore, OTC-I biomatrix is a potential medical device candidate for future clinical trials focusing on wound care management.
Śravaṇ Kumār: Rethinking a Cultural Ideal for Indian Youth
Authors: Baniwal Vikas , Chaudhary Anshu
Published: May 2023

Myths and mythological figures serve as cultural symbols that people live by and emulate. Śravaṇ Kumār is one such mythological figure. He carried his blind parents on his shoulders and, with great hardships, tried to fulfil their wish for a pilgrimage. However, before he could complete the journey, he met a tragic end at the hands of Prince Daśrath. Due to his devotion to his parents, he is revered as an ideal youth in the Indian Hindu context. One wonders what values are conveyed about a society that has, for centuries now, idealised the tragic mythical figure of Śravaṇ Kumār? What could be the underlying fascination with the tragic story of Śravaṇ Kumār, his parents, and the guilt-ridden prince responsible for their tragic deaths and the subsequent ordeal the prince’s son Rām had to endure in accordance with a curse? This paper reinterprets this myth and examines its relevance in contemporary times. The reinterpretation of the myth is further discussed in connection with the relevant psychoanalytic identity development theories, keeping in view the adolescents in the urban metropolitan context in India. The paper concludes by discussing the significance of having relevant mythical and cultural ideals for the identity development of youth.
Assessment of Soil Sustainability Using the LUCAS Database in the Southwest Region of Romania
Authors: Popa Roxana-Gabriela , Șchiopu Emil-Cătălin , Bălăcescu Aniela , Popescu Luminița-Georgeta , Pătrașcu Aurelia
Published: May 2023

To ensure soil sustainability, the European Union considers the mitigation of the ecological, social and economic impacts and the prevention of soil degradation, which is the primary source of the ecosystem. In this respect, Land Use and Coverage Area Frame Survey (LUCAS) studies aim to investigate land use at the community level to gather information necessary for the analysis of the interactions between agriculture, environment and rural landscape and to provide estimates of agricultural areas with main crops. According to data from Eurostat, between May and October 2022, through the use of digital techniques, the levels of land coverage and land use, pastures, as well as irrigation management and structural elements in the landscape, were examined on the ground throughout the European Union. Data on the agricultural environment and soil were collected in the georeferenced points belonging to a representative sample by observing and completing the field form. At the level of the southwest region of Romania, the study was based on the inspection of 274 points by taking soil samples to analyze the quality indicators and identify key species of flowering plants. Data on land coverage and use can be used for a variety of environmental and socioeconomic projects in different fields.
Labor Protection, Enterprise Innovation, and Sustainable Development
Authors: Xu Xinpeng , Zhang Yaqin , Liao Yanran , Fu Xiaopeng
Published: May 2023

As an important way and means to protect the legitimate rights and interests of workers, the implementation of the “Labor Contract Law” is crucial for building and developing harmonious and stable labor relations and the sustainable development of enterprises. Based on the samples of Shanghai and Shenzhen A-share listed enterprises from 2005 to 2013, this paper adopted the difference-in-difference method to empirically test the impact of labor protection on the sustainable development of enterprises and its mechanism. The research finds that the enhancement of labor protection contributes to the sustainable development of highly labor-intensive enterprises, in which enterprise innovation plays a mediating role in the relationship between the two; the heterogeneity test found that labor protection promotes sustainable development more significantly in state-owned enterprises, enterprises in highly market-oriented regions, and large-scale enterprises. This article expands the research on the economic consequences of labor protection and provides empirical inspiration for the improvement of the “Labor Contract Law” system.
Mental Health and Coping Strategies among University Staff during the COVID-19 Pandemic: A Cross–Sectional Analysis from Saudi Arabia
Authors: Mallhi Tauqeer Hussain , Khan Nimra Aslam , Siddique Amina , Salman Muhammad , Bukhari Syed Nasir Abbas , Butt Muhammad Hammad , Khan Faiz Ullah , Khalid Mohammad , Mustafa Zia Ul , Tanveer Nida , Ahmad Naveed , Ahmad Muhammad Masood , Rahman Hidayat Ur , Khan Yusra Habib
Published: May 2023

This study examined psychological health and coping strategies among faculty and staff at a Saudi Arabian university. A web-based self-administered survey was used to assess probable anxiety, depression, post-traumatic stress disorder (PTSD), and coping strategies by using the Generalized Anxiety Disorder-7 (GAD-7), Patient Health Questionnaire-9 (PHQ-9), Impact of Event Scale-Revised (IES-R), and Brief-COPE scale, respectively. Of 502 participants (mean age 36.04 ± 10.32 years, male: 66.3%), 24.1% (GAD-7 ≥ 10) had probable anxiety. Anxiety score was significantly higher in females (p < 0.001), those with a history of COVID-19 infection (p = 0.036), and participants with less work experience (p = 0.019). Approximately 40% of participants met the criteria of probable depression, with females (p < 0.001) and participants with less experience having more depressive symptoms. Around one-fourth (27.7%) of study participants indicated probable PTSD (score ≥ 33), with higher symptoms in females (p <0.001), less experienced staff (p < 0.001), and academic staff (p = 0.006). Correlation analysis indicated a significant positive correlation between anxiety and depression (r = 0.844, p < 0.001), anxiety and PTSD (r = 0.650, p < 0.001), and depression and PTSD (r = 0.676, p < 0.001). Active coping, religious/spiritual coping, and acceptance were common coping strategies, while substance use was the least adopted coping method among the study participants. This study indicated a high prevalence of probable psychological ailments among university staff.
Evaluation of Machine Learning Models for Smart Grid Parameters: Performance Analysis of ARIMA and Bi-LSTM
Authors: Chen Yuanhua , Bhutta Muhammad Shoaib , Abubakar Muhammad , Xiao Dingtian , Almasoudi Fahad M. , Naeem Hamad , Faheem Muhammad
Published: May 2023

The integration of renewable energy resources into smart grids has become increasingly important to address the challenges of managing and forecasting energy production in the fourth energy revolution. To this end, artificial intelligence (AI) has emerged as a powerful tool for improving energy production control and management. This study investigates the application of machine learning techniques, specifically ARIMA (auto-regressive integrated moving average) and Bi-LSTM (bidirectional long short-term memory) models, for predicting solar power production for the next year. Using one year of real-time solar power production data, this study trains and tests these models on performance measures such as mean absolute error (MAE) and root mean squared error (RMSE). The results demonstrate that the Bi-LSTM (bidirectional long short-term memory) model outperforms the ARIMA (auto-regressive integrated moving average) model in terms of accuracy and is able to successfully identify intricate patterns and long-term relationships in the real-time-series data. The findings suggest that machine learning techniques can optimize the integration of renewable energy resources into smart grids, leading to more efficient and sustainable power systems.
Digital Economy, Energy Structure Transformation, and Regional Carbon Dioxide Emissions
Authors: Sun Jingwei , Chen Jingzhu
Published: May 2023

For China to reach its “dual carbon” aim, the digital economy presents both opportunities and obstacles. This paper examines the potential impact of digital economy development on regional carbon dioxide emissions, concluding that while the direct impact on regional carbon dioxide emissions through industrial structure upgrading and technological progress is unclear, the indirect impact through promoting energy structure transformation is both positive and clear. This research experimentally validates the results of a theoretical analysis using panel data from 30 Chinese provinces from 2011 to 2019 and the STIRPAT model and discovers an inverted U-shaped association between digital economy development and regional carbon dioxide emissions. According to the transmission path test, the digital economy primarily achieves its carbon dioxide emission reduction effect by promoting energy structure transformation, while the carbon dioxide emission reduction effect from upgrading industrial institutions and technological progress is insignificant. By region, the expansion of the digital economy in eastern China has a large carbon dioxide emission reduction effect but not in central and western China. The policy implication of this paper is that curbing new carbon dioxide emissions caused by digital infrastructure construction using the digital economy to accelerate energy structure transformation and accelerating digital economy development in central and western China can be effective policy options for the Chinese government to achieve the goal of carbon dioxide emission reduction.
Open Innovation in Agribusiness: Barriers and Challenges in the Transition to Agriculture 4.0
Authors: Silva Francisco Tardelli da , Baierle Ismael Cristofer , Correa Ricardo Gonçalves de Faria , Sellitto Miguel Afonso , Peres Fernanda Araujo Pimentel , Kipper Liane Mahlmann
Published: May 2023

Industry 4.0 digital technologies in agribusiness will enable traditional farming systems to migrate to Agriculture 4.0. Open innovation emerges as an enabler for implementing these technologies and increased sector competitiveness. However, there are still doubts and questions about how technologies and open innovation relate to and will drive Agriculture 4.0. This study identified which digital technologies of Industry 4.0 have more adherence to agribusiness, what the barriers and facilitators for using these technologies are, and how open innovation can increase the competitiveness of agribusiness. The results show that of the Industry 4.0 technologies related to agribusiness, the Internet of Things (IoT) is the most prominent. The main barriers are the users’ need for more knowledge and advanced skills, which evidences the need for investment in training operators. Among the facilitators stand the pre-existence of several technologies, which bring with them already defined basic structures, control of the technology, and communication between systems. To overcome the barriers and enhance the migration to Agriculture 4.0, developing devices, tools, systems, software, and machines is essential. More stakeholders, managers, and practitioners may share such opportunities for innovation in agribusiness through the concept of Open Innovation. To benefit from it, facilitators, managers, and practitioners of agribusiness should search for alternatives for their problems with engineering solutions providers.