bluuebunny/crossref_metadata_2025 huggingface dataset:
Cost of Serving Others: A Moderated Mediation Model of OCB, Ego Depletion, and Service Sabotage
Authors: Hongbo Li , Waqas Muhammad , Tariq Hussain , Yahya Farzan , Marfoh Joseph , Ali Ahsan , Ali Syed Muhammad
Published: March 2021

Taking support from ego-depletion theory, this study examines ego depletion as a mechanism that explains how employees’ organizational citizenship behavior (OCB) leads to antagonistic consequences, i.e., service sabotage. Employees’ positive psychological capital (PsyCap) is considered a moderator. PROCESS macro was used to test all the hypotheses using time-lagged, dyadic data collected from 420 employees and their 112 their supervisors associated with the service industry in China. This study finds that employees’ exhibition of OCB is positively linked to ego depletion, which in turn drives service sabotage behavior. Furthermore, employees’ PsyCap weakens the effect of OCB on employees’ ego depletion. This study highlights the dark side of OCB, the mechanism through which it causes adverse effects, and the moderating effect of PsyCap. It also provides insights to the organizations for managing service sector employees to effectively interact with customers.
Dream Big: Effects of Capitals, Socioeconomic Status, Negative Culture, and Educational Aspirations Among the Senior High School Student Athletes
Authors: Lee Chia-Wen , Yeh Ming-Chia , Hung Huang-Chia
Published: March 2021

To understand the impact of social, financial, cultural capitals, negative culture, and socioeconomic status of families on educational aspiration in the senior high school student athletes, it will be beneficial to promote their career developments. The purpose of this study is to explore the influence of ethnicity, year of sport experience family income, the educational expectations of significant others, and the three aforementioned types of capital on educational aspiration among the senior high school student athletes. This study was conducted with a sample of 262 U-18 male baseball student athletes. Of the participants, 20.20% had attained the qualifications to play on the national team. The results showed that monthly family income positively affected social capital and positively indirectly affected educational aspirations through social capital, whereas monthly family income negatively affected negative culture and positively affected educational aspirations through negative culture. Moreover, social capital positively affected educational aspirations compared with negative culture negatively affected educational aspirations. The results serve as a reference for the formulation of educational policy as it relates to student athletes.
Additional Exergames to Regular Tennis Training Improves Cognitive-Motor Functions of Children but May Temporarily Affect Tennis Technique: A Single-Blind Randomized Controlled Trial
Authors: Šlosar Luka , de Bruin Eling D. , Fontes Eduardo Bodnariuc , Plevnik Matej , Pisot Rado , Simunic Bostjan , Marusic Uros
Published: March 2021

This study evaluated the effects of an exergame program (TennisVirtua-4, Playstation Kinect) combined with traditional tennis training on autonomic regulation, tennis technique, gross motor skills, clinical reaction time, and cognitive inhibitory control in children. Sixty-three children were randomized into four groups (1st – two exergame and two regular trainings sessions/week, 2nd – one exergame and one regular training sessions/week, 3rd – two regular trainings sessions/week, and 4th – one regular training session/week) and compared at baseline, 6-month immediately post intervention and at 1-year follow-up post intervention. At 6-month post intervention the combined exergame and regular training sessions revealed: higher breathing frequency, heart rate (all ps ≤ 0.001) and lower skin conductance levels (p = 0.001) during exergaming; additional benefits in the point of contact and kinetic chain elements of the tennis forehand and backhand technique (all ps ≤ 0.001); negative impact on the shot preparation and the follow-through elements (all ps ≤ 0.017); higher ball skills (as part of the gross motor skills) (p < 0.001); higher percentages of clinical reaction time improvement (1st −9.7% vs 3rd group −7.4% and 2nd −6.6% vs 4th group −4.4%, all ps ≤ 0.003) and cognitive inhibitory control improvement in both congruent (1st −20.5% vs 3rd group −18.4% and 2nd −11.5% vs 4th group −9.6%, all ps ≤ 0.05) and incongruent (1st group −19.1% vs 3rd group −12.5% and 2nd group −11.4% vs 4th group −6.5%, all ps ≤ 0.001) trials. The 1-year follow-up test showed no differences in the tennis technique, clinical reaction time and cognitive inhibitory control improvement between groups with the same number of trainings per week. The findings support exergaming as an additional training tool, aimed to improve important cognitive-motor tennis skills by adding dynamics to the standardized training process. Caution should be placed to planning this training, e.g., in a mesocycle, since exergaming might decrease the improvement of specific tennis technique parts of the trainees. (ClinicalTrials.gov; ID: NCT03946436).
Pandemic Leadership: Sex Differences and Their Evolutionary–Developmental Origins
Authors: Luoto Severi , Varella Marco Antonio Correa
Published: March 2021

The COVID-19 pandemic has caused a global societal, economic, and social upheaval unseen in living memory. There have been substantial cross-national differences in the kinds of policies implemented by political decision-makers to prevent the spread of the virus, to test the population, and to manage infected patients. Among other factors, these policies vary with politicians’ sex: early findings indicate that, on average, female leaders seem more focused on minimizing direct human suffering caused by the SARS-CoV-2 virus, while male leaders implement riskier short-term decisions, possibly aiming to minimize economic disruptions. These sex differences are consistent with broader findings in psychology, reflecting women’s stronger empathy, higher pathogen disgust, health concern, care-taking orientation, and dislike for the suffering of other people—as well as men’s higher risk-taking, Machiavellianism, psychopathy, narcissism, and focus on financial indicators of success and status. This review article contextualizes sex differences in pandemic leadership in an evolutionary framework. Evolution by natural selection is the only known process in nature that organizes organisms into higher degrees of functional order, or counteracts the unavoidable disorder that would otherwise ensue, and is therefore essential for explaining the origins of human sex differences. Differential sexual selection and parental investment between males and females, together with the sexual differentiation of the mammalian brain, drive sex differences in cognition and behavioral dispositions, underlying men’s and women’s leadership styles and decision-making during a global pandemic. According to thesexually dimorphic leadership specialization hypothesis, general psychobehavioral sex differences have been exapted during human evolution to create sexually dimorphic leadership styles. They may be facultatively co-opted by societies and/or followers when facing different kinds of ecological and/or sociopolitical threats, such as disease outbreaks or intergroup aggression. Early evidence indicates that against the invisible viral foe that can bring nations to their knees, the strategic circumspection of empathic feminine health “worriers” may bring more effective and humanitarian outcomes than the devil-may-care incaution of masculine risk-taking “warriors”.
Underwater Docking Approach and Homing to Enable Persistent Operation
Authors: Page Brian R. , Lambert Reeve , Chavez‐Galaviz Jalil , Mahmoudian Nina
Published: March 2021

One of the main limiting factors in deployment of marine robots is the issue of energy sustainability. This is particularly challenging for traditional propeller-driven autonomous underwater vehicles which operate using energy intensive thrusters. One emerging technology to enable persistent performance is the use of autonomous recharging and retasking through underwater docking stations. This paper presents an integrated navigational algorithm to facilitate reliable underwater docking of autonomous underwater vehicles. Specifically, the algorithm dynamically re-plans Dubins paths to create an efficient trajectory from the current vehicle position through approach into terminal homing. The path is followed using integral line of sight control until handoff to the terminal homing method. A light tracking algorithm drives the vehicle from the handoff location into the dock. In experimental testing using an Oceanserver Iver3 and Bluefin SandShark, the approach phase reached the target handoff within 2 m in 48 of 48 tests. The terminal homing phase was capable of handling up to 5 m offsets with approximately 70% accuracy (12 of 17 tests). In the event of failed docking, a Dubins path is generated to efficiently drive the vehicle to re-attempt docking. The vehicle should be able to successfully dock in the majority of foreseeable scenarios when re-attempts are considered. This method, when combined with recent work on docking station design, intelligent cooperative path planning, underwater communication, and underwater power transfer, will enable true persistent undersea operation in the extremely dynamic ocean environment.
Gap Affordance Judgments in Mixed Reality: Testing the Role of Display Weight and Field of View
Authors: Gagnon Holly C. , Zhao Yu , Richardson Matthew , Pointon Grant D. , Stefanucci Jeanine K. , Creem-Regehr Sarah H. , Bodenheimer Bobby
Published: March 2021

Measures of perceived affordances—judgments of action capabilities—are an objective way to assess whether users perceive mediated environments similarly to the real world. Previous studies suggest that judgments of stepping over a virtual gap using augmented reality (AR) are underestimated relative to judgments of real-world gaps, which are generally overestimated. Across three experiments, we investigated whether two factors associated with AR devices contributed to the observed underestimation: weight and field of view (FOV). In the first experiment, observers judged whether they could step over virtual gaps while wearing the HoloLens (virtual gaps) or not (real-world gaps). The second experiment tested whether weight contributes to underestimation of perceived affordances by having participants wear the HoloLens during judgments of both virtual and real gaps. We replicated the effect of underestimation of step capabilities in AR as compared to the real world in both Experiments 1 and 2. The third experiment tested whether FOV influenced judgments by simulating a narrow (similar to the HoloLens) FOV in virtual reality (VR). Judgments made with a reduced FOV were compared to judgments made with the wider FOV of the HTC Vive Pro. The results showed relative underestimation of judgments of stepping over gaps in narrow vs. wide FOV VR. Taken together, the results suggest that there is little influence of weight of the HoloLens on perceived affordances for stepping, but that the reduced FOV of the HoloLens may contribute to the underestimation of stepping affordances observed in AR.
Is Hemoglobin Mass at Age 16 a Predictor for National Team Membership at Age 25 in Cross-Country Skiers and Triathletes?
Authors: Wehrlin Jon Peter , Steiner Thomas
Published: March 2021

We recently measured the development of hemoglobin mass (Hbmass) in 10 Swiss national team endurance athletes between ages 16–19. Level of Hbmass at age 16 was an important predictor for Hbmass and endurance performance at age 19. The aim was to determine how many of these young athletes were still members of Swiss national teams (NT) at age 25, how many already terminated their career (TC), and whether Hbmass at ages 16 and 19 was different between the NT and TC group. We measured Hbmass using the optimized carbon monoxide re-breathing technique in 10 high-performing endurance athletes every 0.5 years beginning at age 16 and ending at age 19. At age 25, two athletes were in the NT group and eight athletes in the TC group. Mean absolute, body weight-, and lean body mass (LBM) related Hbmass at age 16 was 833 ± 61 g, 13.7 ± 0.2 g/kg and 14.2 ± 0.2 g/kg LBM in the NT group and 742 ± 83 g, 12.2 ± 0.7 g/kg and 12.8 ± 0.8 g/kg LBM in the TC group. At age 19, Hbmass was 1,042 ± 89 g, 14.6 ± 0.2 g/kg and 15.4 ± 0.2 g/kg LBM in the NT group and 863 ± 109 g, 12.7 ± 1.1 g/kg and 13.5 ± 1.1 g/kg LBM in the TC group. Body weight- and LBM related Hbmass were higher in the NT group than in the TC group at ages 16 and 19 (p < 0.05). These results indicate, that Hbmass at ages 16 and 19 possibly could be an important predictor for later national team membership in endurance disciplines.
Improved Carotid Elasticity but Altered Central Hemodynamics and Carotid Structure in Young Athletes
Authors: Baumgartner Lisa , Weberruß Heidi , Appel Katharina , Engl Tobias , Goeder Daniel , Oberhoffer-Fritz Renate , Schulz Thorsten
Published: March 2021

Young athletes most often exceed the physical activity recommendations of the World Health Organization. Therefore, they are of special interest for investigating cardiovascular adaptions to exercise. This study aimed to examine the arterial structure and function of young athletes 12–17 years old and compare these parameters to reference values of healthy cohorts. Carotid intima-media thickness (cIMT), carotid diameter, cIMT÷carotid diameter-ratio (cIDR), arterial compliance (AC), elastic modulus (Ep), β stiffness index (β), and carotid pulse wave velocity (PWVβ) were determined using ultrasound in 331 young athletes (77 girls; mean age, 14.6 ± 1.30 years). Central systolic blood pressure (cSBP) and aortic PWV (aPWV) were measured using the oscillometric device Mobil-O-Graph. Standard deviation scores (SDS) of all parameters were calculated according to German reference values. The 75th and 90th percentiles were defined as the threshold for elevated cIMT and arterial stiffness, respectively. Activity behavior was assessed with the MoMo physical activity questionnaire, and maximum power output with a standard cardiopulmonary exercise test. One-sample t-tests were performed to investigate the significant deviations in SDS values compared to the value “0”. All subjects participated in competitive sports for at least 6 h per week (565.6 ± 206.0 min/week). Of the 331 young athletes, 135 (40.2%) had cIMT >75th percentile, 71 (21.5%) had cSBP >90th percentile, and 94 (28.4%) had aPWV>90th percentile. We observed higher cIMT SDS (p < 0.001), cIDR SDS (p = 0.009), and AC SDS (p < 0.001) but lower β SDS (p < 0.001), Ep SDS (p < 0.001), and PWVβ SDS (p < 0.001) compared to the reference cohort. The cSBP SDS (p < 0.001) and aPWV SDS (p < 0.001) were elevated. In conclusion, cIMT and cIDR were higher in young athletes than in a reference cohort. Furthermore, young athletes presented better carotid elasticity and lower arterial stiffness of the carotid artery. However, central arterial stiffness was higher compared to the reference cohort. The thickening of the carotid intima-media complex in combination with a reduction in arterial stiffness indicates a physiological adaptation to exercise in youth.
Assessment of Safety Culture at a Veterinary Teaching Hospital in the United States
Authors: Love Lydia C. , Hedgpeth Mari-Wells , Robertson James B. , Marks Steven L. , Schoenfeld-Tacher Regina M.
Published: March 2021

This study assessed the fidelity of an existing questionnaire regarding attitudes toward safety culture in an academic veterinary hospital setting and gathered baseline data on these attitudes in a local population. A cross-sectional study design was used to evaluate perceptions held by veterinary teaching hospital employees. An established veterinary safety culture survey was modified and administered as a confidential online survey to faculty, house officers, and professional staff of a veterinary teaching hospital in the United States. Confirmatory and exploratory factor analysis were conducted to compare the adapted survey to the established version. Descriptive statistics were used to characterize baseline safety culture. The adapted survey exhibited factor groupings that were mostly in agreement with, but slightly different from, the original instrument. In general, survey respondents outlined positive attitudes toward the various domains of safety culture, though we identified opportunities for improvement in some areas. An adapted veterinary safety culture survey can be applied to a veterinary teaching hospital in the United States to assess baseline data surrounding the culture of safety and to identify opportunities for focused improvement efforts.
Fundamental Matrix Computing Based on 3D Metrical Distance
Authors: Li Xinsheng , Yuan Xuedong
Published: March 2021

To reconstruct point geometry from multiple images, computation of the fundamental matrix is always necessary. With a new optimization criterion, i.e., the re-projective 3D metric geometric distance rather than projective space under RANSAC (Random Sample And Consensus) framework, our method can reveal the quality of the fundamental matrix visually through 3D reconstruction. The geometric distance is the projection error of 3D points to the corresponding image pixel coordinates in metric space. The reasonable visual figures of the reconstructed scenes are shown but only some numerical result were compared, as is standard practice. This criterion can lead to a better 3D reconstruction result especially in 3D metric space. Our experiments validate our new error criterion and the quality of fundamental matrix under the new criterion.
Remote Sensing Image Super-Resolution for the Visual System of a Flight Simulator: Dataset and Baseline
Authors: Ge Wenyi , Wang Zhitao , Wang Guigui , Tan Shihan , Zhang Jianwei
Published: March 2021

High-resolution remote sensing images are the key data source for the visual system of a flight simulator for training a qualified pilot. However, due to hardware limitations, it is an expensive task to collect spectral and spatial images at very high resolutions. In this work, we try to tackle this issue with another perspective based on image super-resolution (SR) technology. First, we present a new ultra-high-resolution remote sensing image dataset named Airport80, which is captured from the airspace near various airports. Second, a deep learning baseline is proposed by applying the generative and adversarial mechanism, which is able to reconstruct a high-resolution image during a single image super-resolution. Experimental results for our benchmark demonstrate the effectiveness of the proposed network and show it has reached satisfactory performances.
Spatial-Temporal Evolution of Scientific Production about Genetically Modified Maize
Authors: Santillán-Fernández Alberto , Salinas-Moreno Yolanda , Valdez-Lazalde José René , Pereira-Lorenzo Santiago
Published: March 2021

Maize is the grain cereal that is the basis of human and animal diets in Mexico and Latin America; it constitutes an essential crop for global food security. The objective of this study was to analyze the spatial–temporal evolution of scientific production on the theme of GMO maize, through a bibliometric analysis of the texts available in the main editorial houses (Elsevier, Scopus, and Springer), open access journal articles database (Conricyt, Scielo, Redalyc, Latindex, Claryvate Analytics, Periodica, and DOAJ), and freely accessible web search engine Google Scholar, to determine the factors that influence the impact of the studies. From 1991 to 2019, 917 texts were found whose spatial–temporal evolution showed a linear growth that concentrated in Latin America (58.56%). The low impact (measured by the number of bibliographic citations) of scientific studies developed in countries of Latin America was related to their publication in journals edited in their own countries and in Spanish, which restricts the constructive criticism of peer review. For the case of Mexico, a spatial discrepancy was also found between research centers and production areas, which limits the transference of technology; and no specialized author in theme of GMO maize was found; the researchers responded to “scientific trends” in agreement with the agrarian policies of the time.
Quality Assessment of Raw Honey Issued from Eastern Romania
Authors: Albu Aida , Radu-Rusu Cristina-Gabriela , Pop Ioan Mircea , Frunza Gabriela , Nacu Gherasim
Published: March 2021

Romania is known among the main European honey producers, due to the variety of landforms as well as the diversity of the flora. Thirty-four honey samples of the acacia, linden and multifloral types, produced in eastern Romania and collected during 2013–2018, were physico-chemically analyzed using methods provided by the national and EU standards. The results of water-insoluble solids, color and refractive index were found to be 0.023–0.131%, 0.3–76.4 mm Pfund and 1.485–1.499, respectively. The moisture content ranged between 15.20% and 20.77%, solid substances content ranged between 79.23% and 84.80% and total soluble substances content ranged between 77.83 °Brix and 83.26 °Brix. The obtained values of specific gravity were from 1.414 to 1.450 g/cm3, pH ranged from 3.673 to 5.503 and free acidity ranged between 2.4 meq and 50 meq kg−1. The ash content and the electrical conductivity varied between 0.030 and 0.543% and 130 and 679 µS cm−1, respectively. Pearsonʹs correlation analysis showed an intense association of the ash content with electrical conductivity (r = 0.81). Our findings reveal the qualitative level of Romanian honey and the variation in quality parameters due to factors such as geographical region, climatic conditions, botanical origin and handling or storage conditions.
Potassium: A Vital Macronutrient in Potato Production—A Review
Authors: Torabian Shahram , Farhangi-Abriz Salar , Qin Ruijun , Noulas Christos , Sathuvalli Vidyasagar , Charlton Brian , Loka Dimitra A.
Published: March 2021

Potassium (K) is a primary macronutrient for overall plant growth, yield potential, product quality and stress resistance of crops. Potato (Solanum tuberosum L.) crops require a high amount of potassium to achieve the ideal yield and quality. Therefore, the determination of optimum K rate and efficient source for potato is necessary because K affects crop physiological processes, dry matter production, cooking, and processing requirements. Through modeling on the pooled data extracted from 62 studies, the highest tuber yields might be obtained at an exchangeable soil K level of 200 mg kg−1 approximately, dependent on soil pH, texture, and organic matter. Through modeling on the data of 48 studies, it also revealed that application of potassium sulfate (K2SO4) and potassium chloride (KCl) at rates of 200 kg ha−1 and potassium nitrate (KNO3) at a rate of 100 kg ha−1 might achieve the ideal yield, implying the importance of K sources in potato production. However, these values (either soil exchangeable K content, or fertilizer rates) might not be applicable in a specific growing environment for a specific potato variety. It seems that there is no discrimination among split, pre-plant or in-season application of K, although pre-plant fertilization might be a trustworthy strategy for economic tuber yield. Owing to the luxury consumption of K by potato crop, a combination of factors, including soil exchangeable K level, petiole K concentration, crop removal amount, soil conditions, management practices, climatic conditions, and potato variety, should be considered in order to make rational K fertilizer recommendations.
Effect of Ozonated Fertigation in Pepper Cultivation under Greenhouse Conditions
Authors: Colunje Julian , Garcia-Caparros Pedro , Moreira Jorge F. , Lao María Teresa
Published: March 2021

Ozone has a high oxidizing power avoiding the presence of residues, so it is a good candidate for use in organic farming. However, its application in fertigation has been little studied. Two experiments were conducted simultaneously to check the aims of this work. The aim of the first experiment was to study pepper response under the nutrient solution supply and the application of O3 and its interactions, and the aim of the second experiment was to determine the optimal dosage of O3 to be supplied, from the point of view of plant development and nutritional and physiological status in both experiments. We conclude that O3 supply via fertigation increased pepper biomass production. According to the results obtained, we recommend that the optimal dosage of O3 in pepper plants be from 0.18 to 0.36 mg L−1 due to its higher associated biomass production. Under these dosages, pepper plants showed a higher leaf area and higher photosynthetic pigment concentration. Similarly, under these dosages, N, P, and K plant uptake were higher, allowing a higher synthesis of bioassimilates.
Exogenous Auxin-Mediated Salt Stress Alleviation in Faba Bean (Vicia faba L.)
Authors: Abdel Latef Arafat Abdel Hamed , Tahjib-Ul-Arif Md. , Rhaman Mohammad Saidur
Published: March 2021

Auxin not only controls the development processes, but also regulates the stress responses of plants. In this investigation, we explored the potential roles of exogenously applied indole-3-acetic acid (IAA) in conferring salt tolerance in the faba bean (Vicia faba L.). Our results showed that foliar application of IAA (200 ppm) to salt-exposed (60 mM and 150 mM NaCl) plants promoted growth, which was evidenced by enhanced root–stem traits. IAA application ensured better osmotic protection in salt-stressed plants which was supported by reduced proline and enhanced soluble sugar, soluble protein, and total free amino acid contents in the roots, stem, and seeds. IAA application also increased the number of nodules in salt-stressed plants, which may facilitate better nitrogen assimilation. Moreover, IAA mediated improvements in mineral homeostasis (K+, Ca2+, and Mg2+) and the translocation of Na+, while it also inhibited excessive accumulation of Na+ in the roots. Salt-induced oxidative damage resulted in increased accumulation of malondialdehyde, whereas IAA spraying relegated malondialdehyde by improving antioxidant enzymes, including superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Together, these results together with a principal component analysis uncovered that foliar spraying of IAA alleviated the antagonistic effects of salt stress via enhancing osmolyte accumulation, ionic homeostasis, and antioxidant activity. Finally, exogenous IAA enhanced the yield of broad beans under high salinity conditions.
Cover Crop Introduction in a Mediterranean Maize Cropping System. Effects on Soil Variables and Yield
Authors: Silvestri Nicola , Grossi Nicola , Mariotti Marco , Arduini Iduna , Guglielminetti Lorenzo , Raffaelli Michele , Cardelli Roberto
Published: March 2021

Cover crops (CCs) are able to affect subsequent crop behaviour by acting on many soil variables and affecting the dynamics of different ecological processes. This study aimed to investigate the effects of introducing CC in continuous-maize cropping systems within Mediterranean areas. The experimental site was located in Central Italy, on a sandy loam and the research activity was carried out over two years (2019–2020). The two cropping systems in comparison differed from each other in terms of the CC cultivation: TR (treated, with CC) and CO (control, without CC). In both years, we observed a significant reduction (p < 0.05) of soil nitrate and water content for the TR system. In the shallowest layer (0–30 cm), nitrate content was reduced by up −80% and −65% (July 2019 and 2020), whereas soil moisture showed decreases ranging from −13% (July 2019) to −34% (May 2019). In 2019, the TR-maize (Zea mays L.) yield was statistically lower than CO (−443 g dm m−2), whereas in 2020 the yields of the two systems resulted statistically equivalent. This different behaviour can be explained with the serious delay in the CC sowing occurred in 2019 (12 December). Conversely, an increase in the apparent remaining N in the soil (+140 and +133 kg N ha−1 for 2019 and 2020, respectively) and in the C (carbon) inputs (+4.78 and +7.39 t dm ha−1 of biomass) were pointed out for the TR system. The large use of inputs in Mediterranean maize cropping systems limited some of the benefits from CCs, but their suitability has to be evaluated by considering all the involved effects, some of which need a long time to become appreciable.
Crocus sativus L. Ecotypes from Mediterranean Countries: Phenological, Morpho-Productive, Qualitative and Genetic Traits
Authors: Cardone Loriana , Castronuovo Donato , Perniola Michele , Cicco Nunzia , Molina Rosa V. , Renau-Morata Begoña , Nebauer Sergio G. , Candido Vincenzo
Published: March 2021

The characterization of C. sativus ecotypes is of great interest for preserving them from a possible genetic erosion due to the decrease of European cultivation surface. In this study, we evaluated four ecotypes from Italy (Sardinia and Abruzzo), Spain (Castilla-La Mancha), and Greece (Kozani) in order to detect the existence of variability and promote the biodiversity of this crop. Thirty-one traits related to saffron flowering, flower morphology, production of spice and daughter corms, vegetative development (leaf and corm traits), and spice quality, were evaluated. In addition, a genetic analysis through three PCR-based approaches, SSRs, RAPD, and SRAP was assessed. Results highlighted a phenotypic variation among ecotypes during two consecutive years. All the studied parameters were influenced by the ecotype except for the stamen length, color coordinates of tepals, leaf length, and leaf number per plant. Sardinia had a longer flowering interval, earlier flowering, and higher spice yield and quality than the other corm origins. The maximum values of morphological traits, such as stigma length, dry weight of stigmas, tepals, flowers and leaves, leaf area, and daughter corm weight were observed in the Abruzzo ecotype. Principal component analysis (PCA) showed a clear separation among ecotypes, in which Sardinia and Spain showed more similarities than Abruzzo and Kozani. Significant negative correlation was found between days to flower with stigma yield and quality. However, we could not find molecular markers discriminating among corm origins. In conclusion, this study suggests the importance of C. sativus ecotypes as precious source of biodiversity and bioactive compounds, and of their enhancement as fundamental prerequisite for a sustainable development strategy and as an agricultural diversification opportunity for growers.
Improving Grain Zinc Concentration in Wetland and Upland Rice Varieties Grown under Waterlogged and Well-Drained Soils by Applying Zinc Fertilizer
Authors: Tuiwong Patcharin , Lordkaew Sithisavet , Prom-u-thai Chanakan
Published: March 2021

The objective of this study was to evaluate the responses in grain yield and zinc concentration of wetland and upland rice varieties to Zn fertilizer application and different growing conditions. The wetland (Chainat 1; CNT1) and upland (Kum Hom CMU; KH CMU) rice varieties were grown under waterlogged and well-drained soil conditions with or without Zn fertilizer application. Zinc fertilizer (ZnSO4) was applied at 0 and 60 kg ha−1 in three stages at tillering, booting, and flowering. In the wetland variety, CNT1, grain yield decreased by 18.0% in the well-drained soil compared to the waterlogged conditions, but there was an 8.9% decrease in grain yield in the waterlogged soil compared to the well-drained soil in the upland variety, KH CMU. Applying Zn fertilizer affected yields differently between the varieties, decreasing grain yield by 11.9% in CNT1 while having no effect in KH CMU. For grain Zn concentrations in brown rice, applying Zn fertilizer increased Zn concentration by 16.5–23.1% in CNT1 and KH CMU under both growing conditions. In the well-drained soil, applying Zn fertilizer increased straw Zn concentration by 51.6% in CNT1 and by 43.4% in KH CMU compared with the waterlogged conditions. These results indicated that the wetland and upland rice varieties responded differently to Zn fertilizer application when grown in different conditions. Applying Zn fertilizer in the appropriate rice variety and growing conditions would help farmers to improve both the desirable grain yield and Zn concentration in rice.
Exploring Climate Change Effects on Vegetation Phenology by MOD13Q1 Data: The Piemonte Region Case Study in the Period 2001–2019
Authors: Sarvia Filippo , De Petris Samuele , Borgogno-Mondino Enrico
Published: March 2021

Rising temperature, rainfall, and wind regime changes, increasing of frequency and intensity of extreme events are only some of the effects of climate change affecting the agro-forestry sector. Earth Observation data from satellite missions (often available for free) can certainly support analysis of climate change effects on vegetation, making possible to improve land management in space and time. Within this context, the present work aims at investigating natural and agricultural vegetation, as mapped by Corine Land Cover (CLC) dataset, focusing on phenological metrics trends that can be possibly conditioned by the ongoing climate-change. The study area consists of the entire Piemonte region (NW-Italy). MOD13Q1-v6 dataset from TERRA MODIS mission was used to describe pluri-annual (2001–2019) phenological behavior of vegetation focusing on the following CLC classes: Non-irrigated arable land, Vineyards, Pastures, and Forests. After computing and mapping some phenological metrics as derivable from the interpretation of at-pixel level NDVI (Normalized Difference Vegetation Index) temporal profile, we found that the most significant one was the maximum annual NDVI (MaxNDVI). Consequently, its trend was analyzed at CLC class level for the whole Piemonte region. Natural and semi-natural vegetation classes (Pastures and Forests) were furtherly investigated testing significance of the Percent Total Variation (TV%) of MaxNDVI in the period 2001–2019 for different altitude classes. Results proved that Non-irrigated arable land showed a not significant trend of MaxNDVI; differently, vineyards and forests showed a significant increasing one. Concerning TV%, it was found that it increases with altitude for the Forests CLC class, while it decreases with altitude for the pastures class.
Brassinosteroid Signaling Pathways Interplaying with Diverse Signaling Cues for Crop Enhancement
Authors: Hwang Hyeona , Ryu Hojin , Cho Hyunwoo
Published: March 2021

The signaling pathways of brassinosteroids (BRs), a unique plant steroid hormone, are critically involved in a diverse range of plant growth and developmental processes as well as many important agronomic traits. Recent advances in the understanding of BR biosynthetic and signaling pathways in model organisms and crops have increased the feasibility of modulating BR responses in crop plants to enhance adaptation to various vulnerable environmental changes. In particular, the identification and functional analysis of BR signaling components in rice (Oryza sativa) present the possibility of their utilization to improve many agricultural traits involved in crop yields. In this review, we summarize recent advances and progress in the understanding of the BR signaling pathway and its interactions with diverse internal and external signaling cues. We also discuss how these physiological modulations of BR and the abundant signaling crosstalk can be applied to enhance rice productivity through the manipulation of plant architecture and fine-tuning of stress responses. Finally, we discuss how the complex regulation of BR signaling pathways could favor application in the molecular design of plant growth and development, precise breeding strategies, and cultivation methods for rice crop improvement.
Chromosome Abnormalities and Fertility in Domestic Bovids: A Review
Authors: Iannuzzi Alessandra , Parma Pietro , Iannuzzi Leopoldo
Published: March 2021

After discovering the Robertsonian translocation rob(1;29) in Swedish red cattle and demonstrating its harmful effect on fertility, the cytogenetics applied to domestic animals have been widely expanded in many laboratories in order to find relationships between chromosome abnormalities and their phenotypic effects on animal production. Numerical abnormalities involving autosomes have been rarely reported, as they present abnormal animal phenotypes quickly eliminated by breeders. In contrast, numerical sex chromosome abnormalities and structural chromosome anomalies have been more frequently detected in domestic bovids because they are often not phenotypically visible to breeders. For this reason, these chromosome abnormalities, without a cytogenetic control, escape selection, with subsequent harmful effects on fertility, especially in female carriers. Chromosome abnormalities can also be easily spread through the offspring, especially when using artificial insemination. The advent of chromosome banding and FISH-mapping techniques with specific molecular markers (or chromosome-painting probes) has led to the development of powerful tools for cytogeneticists in their daily work. With these tools, they can identify the chromosomes involved in abnormalities, even when the banding pattern resolution is low (as has been the case in many published papers, especially in the past). Indeed, clinical cytogenetics remains an essential step in the genetic improvement of livestock.
Keeping Dairy Cows for Longer: A Critical Literature Review on Dairy Cow Longevity in High Milk-Producing Countries
Authors: Dallago Gabriel M. , Wade Kevin M. , Cue Roger I. , McClure J T. , Lacroix René , Pellerin Doris , Vasseur Elsa
Published: March 2021

The ability of dairy farmers to keep their cows for longer could positively enhance the economic performance of the farms, reduce the environmental footprint of the milk industry, and overall help in justifying a sustainable use of animals for food production. However, there is little published on the current status of cow longevity and we hypothesized that a reason may be a lack of standardization and an over narrow focus of the longevity measure itself. The objectives of this critical literature review were: (1) to review metrics used to measure dairy cow longevity; (2) to describe the status of longevity in high milk-producing countries. Current metrics are limited to either the length of time the animal remains in the herd or if it is alive at a given time. To overcome such a limitation, dairy cow longevity should be defined as an animal having an early age at first calving and a long productive life spent in profitable milk production. Combining age at first calving, length of productive life, and margin over all costs would provide a more comprehensive evaluation of longevity by covering both early life conditions and the length of time the animal remains in the herd once it starts to contribute to the farm revenues, as well as the overall animal health and quality of life. This review confirms that dairy cow longevity has decreased in most high milk-producing countries over time and its relationship with milk yield is not straight forward. Increasing cow longevity by reducing involuntary culling would cut health costs, increase cow lifetime profitability, improve animal welfare, and could contribute towards a more sustainable dairy industry while optimizing dairy farmers’ efficiency in the overall use of resources available.
Impact of Ambient Temperature Sample Storage on the Equine Fecal Microbiota
Authors: Martin de Bustamante Michelle , Plummer Caryn , MacNicol Jennifer , Gomez Diego
Published: March 2021

Sample storage conditions are an important factor in fecal microbiota analyses in general. The objective of this study was to investigate the effect of sample storage at room temperature on the equine fecal microbiota composition. Fecal samples were collected from 11 healthy horses. Each sample was divided into 7 sealed aliquots. One aliquot was immediately frozen at −80 °C; the remaining aliquots were stored at room temperature (21 to 22 °C) with one transferred to the freezer at each of the following time points: 6, 12, 24, 48, 72 and 96 h. The Illumina MiSeq sequencer was used for high-throughput sequencing of the V4 region of the 16S rRNA gene. Fibrobacteraceae (Fibrobacter) and Ruminococcaceae (Ruminococcus) were enriched in samples from 0 h and 6 h, whereas taxa from the families Bacillaceae, Planococcaceae, Enterobacteriaceae and Moraxellaceae were enriched in samples stored at room temperature for 24 h or greater. Samples frozen within the first 12 h after collection shared similar community membership. The community structure was similar for samples collected at 0 h and 6 h, but it was significantly different between samples frozen at 0 h and 12 h or greater. In conclusion, storage of equine fecal samples at ambient temperature for up to 6 h before freezing following sample collection had minimal effect on the microbial composition. Longer-term storage at ambient temperature resulted in alterations in alpha-diversity, community membership and structure and the enrichment of different taxa when compared to fecal samples immediately frozen at −80 °C.
The Role of Mustelids in the Transmission of Sarcocystis spp. Using Cattle as Intermediate Hosts
Authors: Prakas Petras , Balčiauskas Linas , Juozaitytė-Ngugu Evelina , Butkauskas Dalius
Published: March 2021

There is a lack of research on the role of mustelids in the transmission of various Sarcocystis spp. In the present study we tested the hypothesis that widespread mustelids in Lithuania could be involved in the transmission of Sarcocystis spp. using cattle as intermediate hosts. In 2016–2020, intestinal samples of 84 mustelids were examined. Sarcocystis spp. were identified by species-specific PCR targeting the cox1 gene and subsequent sequencing. Under a light microscope, oocysts/sporocysts of Sarcocystis spp. were observed in 40 samples (47.6%), while using molecular methods, they were detected in 75 animals (89.3%). Four Sarcocystis spp. were identified in the intestinal samples of American mink (Neovisonvison), Beech marten (Martes foina), European pine marten (Martes martes), European badger (Meles meles) and European polecat (Mustela putorius). The prevalence of predominant Sarcocystis spp., S. bovifelis (89.3%) and S. cruzi (73.8%) was significantly higher than that of S. hirsuta (3.6%) and S. hominis (1.2%). In an individual sample, most frequently two Sarcocystis spp. were identified (69.0%), then a single species (15.5%) and three species (4.8%). The present study provides strong evidence that mustelids serve as definitive hosts for Sarcocystis spp. using cattle as intermediate hosts.
Animal Welfare Implications of Digital Tools for Monitoring and Management of Cattle and Sheep on Pasture
Authors: Herlin Anders , Brunberg Emma , Hultgren Jan , Högberg Niclas , Rydberg Anna , Skarin Anna
Published: March 2021

The opportunities for natural animal behaviours in pastures imply animal welfare benefits. Nevertheless, monitoring the animals can be challenging. The use of sensors, cameras, positioning equipment and unmanned aerial vehicles in large pastures has the potential to improve animal welfare surveillance. Directly or indirectly, sensors measure environmental factors together with the behaviour and physiological state of the animal, and deviations can trigger alarms for, e.g., disease, heat stress and imminent calving. Electronic positioning includes Radio Frequency Identification (RFID) for the recording of animals at fixed points. Positioning units (GPS) mounted on collars can determine animal movements over large areas, determine their habitat and, somewhat, health and welfare. In combination with other sensors, such units can give information that helps to evaluate the welfare of free-ranging animals. Drones equipped with cameras can also locate and count the animals, as well as herd them. Digitally defined virtual fences can keep animals within a predefined area without the use of physical barriers, relying on acoustic signals and weak electric shocks. Due to individual variations in learning ability, some individuals may be exposed to numerous electric shocks, which might compromise their welfare. More research and development are required, especially regarding the use of drones and virtual fences.
Ishige okamurae Suppresses Trimethyltin-Induced Neurodegeneration and Glutamate-Mediated Excitotoxicity by Regulating MAPKs/Nrf2/HO-1 Antioxidant Pathways
Authors: Kwon Oh Yun , Lee Seung Ho
Published: March 2021

Many neurodegenerative diseases have several similar cellular dysregulations. We investigated the inhibitory role of Ishige okamurae, an edible brown alga, on neurodegenerative processes by estimating the effects of Ishige okamurae on excitotoxicity induced by glutamate in vitro and neurodegeneration induced by trimethyltin (TMT) in vivo. This study aimed to describe the molecular mechanisms responsible for the mediating anti-neurodegenerative effects of Ishige okamurae extract (IOE). The oral administration of IOE to TMT-injected mice impeded the TMT-mediated short- and long-term memory impairments investigated by the Morris water maze and Y-maze test. IOE attenuated TMT-mediated cellular apoptosis and the expression of brain-derived neurotrophic factor, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in mice brains. Glutamate-induced apoptosis and the expression of reactive oxygen species, Nrf2, and HO-1 in HT22 cells were also attenuated by IOE. In addition, TMT- and glutamate-induced phosphorylation of mitogen-activated protein kinases (MAPKs) in mouse brain tissues and HT22 cells were attenuated by the treatment of IOE. In HT22 cells, administration of MAPK inhibitors recovered the glutamate induced by the expression of Nrf2, HO-1, and cellular dysregulation to the equal extent to IOE administration. Taken together, these results suggest that IOE could attenuate neurodegenerative processes, such as TMT- and glutamate-mediated neuronal dysregulation, by regulating MAPKs/Nrf-2/HO-1 antioxidant pathways.
Intra-Individual Variation and Reliability of Biomarkers of the Antioxidant Defense System by Considering Dietary and Lifestyle Factors in Premenopausal Women
Authors: Jungert Alexandra , Frank Jan
Published: March 2021

Epidemiological studies frequently rely on a single biomarker measurement to assess the relationship between antioxidant status and diseases. This bears an inherent risk for misclassification, if the respective biomarker has a high intra-individual variability. The present study investigates the intra-individual variation and reliability of enzymatic and non-enzymatic biomarkers of the antioxidant system in premenopausal women. Forty-four apparently healthy females provided three consecutive fasting blood samples in a four-week rhythm. Analyzed blood biomarkers included Trolox equivalent antioxidant capacity (TEAC), catalase, glutathione peroxidase, glutathione, vitamin C, bilirubin, uric acid, coenzyme Q10, tocopherols, carotenoids and retinol. Intra- and inter-individual variances for each biomarker were estimated before and after adjusting for relevant influencing factors, such as diet, lifestyle and use of contraceptives. Intraclass correlation coefficient (ICC), index of individuality, reference change value and number of measurements needed to confine attenuation in regression coefficients were calculated. Except for glutathione and TEAC, all biomarkers showed a crude ICC ≥ 0.50 and a high degree of individuality indicating that the reference change value is more appropriate than population-based reference values to scrutinize and classify intra-individual changes. Apart from glutathione and TEAC, between 1 and 9 measurements were necessary to reduce attenuation in regression coefficients to 10%. The results indicate that the majority of the assessed biomarkers have a fair to very good reliability in healthy premenopausal women, except for glutathione and TEAC. To assess the status of the antioxidant system, the use of multiple measurements and biomarkers is recommended.