bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of an Inkjet-Printed Rotary Bellows Actuator and Simulation of its Time-Dependent Deformation Behavior
Authors: Dämmer Gabriel , Lackner Michael , Laicher Sonja , Neumann Rüdiger , Major Zoltán
Published: June 2021

State-of-the-art Additive Manufacturing processes such as three-dimensional (3D) inkjet printing are capable of producing geometrically complex multi-material components with integrated elastomeric features. Researchers and engineers seeking to exploit these capabilities must handle the complex mechanical behavior of inkjet-printed elastomers and expect a lack of suitable design examples. We address these obstacles using a pneumatic actuator as an application case. First, an inkjet-printable actuator design with elastomeric bellows structures is presented. While soft robotics research has brought forward several examples of inkjet-printed linear and bending bellows actuators, the rotary actuator described here advances into the still unexplored field of additively manufactured pneumatic lightweight robots with articulated joints. Second, we demonstrate that the complex structural behavior of the actuator’s elastomeric bellows structure can be predicted by Finite Element (FE) simulation. To this end, a suitable hyperviscoelastic material model was calibrated and compared to recently published models in a multiaxial-state-of-stress relaxation experiment. To verify the material model, Finite Element simulations of the actuator’s deformation behavior were conducted, and the results compared to those of corresponding experiments. The simulations presented here advance the materials science of inkjet-printed elastomers by demonstrating use of a hyperviscoelastic material model for estimating the deformation behavior of a prototypic robotic component. The results obtained contribute to the long-term goal of additively manufactured and pneumatically actuated lightweight robots.
Fully Polymeric Domes as High-Stroke Biasing System for Soft Dielectric Elastomer Actuators
Authors: Neu Julian , Hubertus Jonas , Croce Sipontina , Schultes Günter , Seelecke Stefan , Rizzello Gianluca
Published: June 2021

The availability of compliant actuators is essential for the development of soft robotic systems. Dielectric elastomers (DEs) represent a class of smart actuators which has gained a significant popularity in soft robotics, due to their unique mix of large deformation (>100%), lightweight, fast response, and low cost. A DE consists of a thin elastomer membrane coated with flexible electrodes on both sides. When a high voltage is applied to the electrodes, the membrane undergoes a controllable mechanical deformation. In order to produce a significant actuation stroke, a DE membrane must be coupled with a mechanical biasing system. Commonly used spring-like bias elements, however, are generally made of rigid materials such as steel, and thus they do not meet the compliance requirements of soft robotic applications. To overcome this issue, in this paper we propose a novel type of compliant mechanism as biasing elements for DE actuators, namely a three-dimensional polymeric dome. When properly designed, such types of mechanisms exhibit a region of negative stiffness in their force-displacement behavior. This feature, in combination with the intrinsic softness of the polymeric material, ensures large actuation strokes as well as compliance compatibility with soft robots. After presenting the novel biasing concept, the overall soft actuator design, manufacturing, and assembly are discussed. Finally, experimental characterization is conducted, and the suitability for soft robotic applications is assessed.
Metabolizable Protein: 1. Predicting Equations to Estimate Microbial Crude Protein Synthesis in Small Ruminants
Authors: Santos Stefanie Alvarenga , de Carvalho Gleidson Giordano Pinto , Azevêdo José Augusto Gomes , Zanetti Diego , Santos Edson Mauro , Pereira Mara Lucia Albuquerque , Pereira Elzania Sales , Pires Aureliano José Vieira , Valadares Filho Sebastião de Campos , Teixeira Izabelle Auxiliadora Molina de Almeida , Tosto Manuela Silva Libânio , Leite Laudi Cunha , Mariz Lays Débora Silva
Published: June 2021

Microbial crude protein (MCP) produced in rumen could be estimated by a variety of protocols of experimental sampling and analysis. However, a model to estimate this value is necessary when protein requirements are calculated for small ruminants. This model could be useful to calculate rumen degradable protein (RDP) requirements from metabolizable protein (MP). Then, our objective was to investigate if there is a difference in MCP efficiency between sheep and goats, and to fit equations to predict ruminal MCP production from dietary energy intake. The database consisted of 19 studies with goats (n = 176) and sheep (n = 316), and the variables MCP synthesis (g/day), total digestible nutrients (TDN), and organic matter (OM) intakes (g/day), and OM digestibility (g/kg DM) were registered for both species. The database was used for two different purposes, where 70% of the values were sorted to fit equations, and 30% for validation. A meta-analytical procedure was carried out using the MIXED procedure of SAS, specie was considered as the fixed dummy effect, and the intercept and slope nested in the study were considered random effects. No effect of specie was observed for the estimation of MCP from TDN, digestible Organic Matter (dOM), or metabolizable energy (ME) intakes (P > 0.05), considering an equation with or without an intercept. Therefore, single models including both species at the same fitting were validated. The following equations MCP (g/day) = 12.7311 + 59.2956 × TDN intake (AIC = 3,004.6); MCP (g/day) = 15.7764 + 62.2612 × dOM intake (AIC = 2,755.1); and MCP (g/day) = 12.7311 + 15.3000 × ME intake (AIC = 3,007.3) presented lower values for the mean square error of prediction (MSEP) and its decomposition, and similar values for the concordance correlation coefficient (CCC) and for the residual mean square error (RMSE) when compared with equations fitted without an intercept. The intercept and slope pooled test was significant for equations without an intercept (P < 0.05), indicating that observed and predicted data differed. In contrast, predicted and observed data for complete equations were similar (P > 0.05).
Bat E-Commerce: Insights Into the Extent and Potential Implications of This Dark Trade
Authors: Chaber Anne-Lise , Amstrong Kyle N. , Wiantoro Sigit , Xerri Vanessa , Caraguel Charles , Boardman Wayne S. J. , Nielsen Torben D.
Published: June 2021

Little is known about the global bat souvenir trade despite previous research efforts into bat harvest for bushmeat. We screened eBay listings of bats in Australia, Canada, Italy, Switzerland, United Kingdom and USA to assess the nature and extent of the online offers. A total of 237 listings were retrieved in between the 11th and 25th of May 2020 with a median price per item of US$38.50 (range: US$8.50–2,500.00). Items on offer were mostly taxidermy (61.2%) or skull (21.1%) specimens. Overall, 32 different species of bat were advertised, most of which (n = 28) are listed as “Least Concern” on the International Union for Conservation of Nature (IUCN) Red List. One species (Nycteris javanica) is classified as “Vulnerable” and one (Eidolon helvum) as “Near Threatened.” Pteropus spp. specimens were the most expensive specimens on offer and the conservations status of these species may range from “Critically Endangered” to “Data Deficient” by IUCN and the entire genus is listed in the Appendix II by the Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES). However, the exact species concerned, and their respective conservation status, could not be confirmed based on the listings' photos. The sourcing of bat was restricted to mostly South-East Asian countries (a third of items sourced from Indonesia) and to two African countries. Our survey revealed that the online offer of bat products is diverse, abundant, and facilitated by worldwide sellers although most offered bats species are from South-East Asia. With a few exceptions, the species on offer were of little present conservation concern, however, many unknowns remain on the potential animal welfare, biosecurity, legal implications, and most importantly public health risks associated with this dark trade.
Effects of Alpha-Connexin Carboxyl-Terminal Peptide (aCT1) and Bowman-Birk Protease Inhibitor (BBI) on Canine Oral Mucosal Melanoma (OMM) Cells
Authors: Sato Ayami , da Fonseca Ivone Izabel Mackowiak , Nagamine Márcia Kazumi , de Toledo Gabriela Fernandes , Olio Rennan , Hernandez-Blazquez Francisco Javier , Yano Tomohiro , Yeh Elizabeth Shinmay , Dagli Maria Lucia Zaidan
Published: June 2021

Oral mucosal melanomas (OMM) are aggressive cancers in dogs, and are good models for human OMM. Gap junctions are composed of connexin units, which may have altered expression patterns and/or subcellular localization in cancer cells. Cell-to-cell communication by gap junctions is often impaired in cancer cells, including in melanomas. Meanwhile, the upregulated expression of the gap junction protein connexin 43 (Cx43) inhibits melanoma progression. The α-connexin carboxyl-terminal (aCT1) peptide reportedly maintains Cx43 expression and cell-cell communication in human mammary cells and increases the communication activity through gap junctions in functional assays, therefore causing decreased cell proliferation. The Bowman-Birk protease inhibitor (BBI), a component of soybeans, induces Cx43 expression in several tumor cells as a trypsin–chymotrypsin inhibition function, with antineoplastic effects. This study investigated the effect of aCT1 peptide and BBI treatment, alone or in combination, on TLM1 canine melanoma cell viability. Cell viability after treatment with aCT1, the reverse sequence peptide (R-pep), and/or BBI for 5 days was analyzed by PrestoBlue assay. Immunofluorescence was used to observe Cx43 localization and expression. aCT1 (200 μM) alone did not significantly decrease cell viability in TLM1 cells, whereas BBI (400 μg/ml) alone significantly decreased the TLM1 viability. Combined treatment with both aCT1 (200 μM) and BBI (400 μg/ml) significantly decreased cell viability in TLM1 cells. Cx43 expression, as identified by immunostainings in TLM1 cells, was increased in the cell membrane after the combination treatment with BBI and aCT1. This dual treatment can be combined to achieve the anticancer activity, possibly by increasing Cx 43 expression and affecting Cx43 migration to the cell membrane. In conclusion, a treatment strategy targeting Cx43 with BBI and aCT1 may possibly lead to new effective therapies for canine OMM.
Influence of Novel Airframe Technologies on the Feasibility of Fully-Electric Regional Aviation
Authors: Karpuk Stanislav , Elham Ali
Published: June 2021

The feasibility of regional electric aviation to reduce environmental impact highly depends on technological advancements of energy storage techniques, available battery energy density, and high-power electric motor technologies. However, novel airframe technologies also strongly affect the feasibility of a regional electric aircraft. In this paper, the influence of novel technologies on the feasibility of regional electric aviation was investigated. Three game-changing technologies were applied to a novel all-electric regional aircraft: active flow control, active load alleviation, and novel materials and structure concepts. Initial conceptual design and mission analysis of the aircraft was performed using the aircraft design framework SUAVE, and the sensitivity of the most important technologies on the aircraft characteristics and performance were studied. Obtained results were compared against a reference ATR-72 aircraft. Results showed that an all-electric aircraft with airframe technologies might be designed with the maximum take-off weight increase of 50% starting from the battery pack energy density of 700 Wh/kg. The overall emission level of an all-electric aircraft with novel technologies is reduced by 81% compared to the ATR-72. On the other hand, novel technologies do not contribute to the reduction in Direct Operating Costs (DOC) starting from 700 Wh/kg if compared to an all-electric aircraft without technologies. An increase in DOC ranges from 43% to 30% depending on the battery energy density which creates a significant market obstacle for such type of airplanes. In addition, the aircraft shows high levels of energy consumption which concerns its energy efficiency. Finally, the sensitivity of DOC to novel technologies and sensitivities of aircraft characteristics to each technology were assessed.
Bioactive Compounds of Tomato Fruit in Response to Salinity, Heat and Their Combination
Authors: Botella María Ángeles , Hernández Virginia , Mestre Teresa , Hellín Pilar , García-Legaz Manuel Francisco , Rivero Rosa María , Martínez Vicente , Fenoll José , Flores Pilar
Published: June 2021

In light of foreseen global climatic changes, we can expect crops to be subjected to several stresses that may occur at the same time, but information concerning the effect of long-term exposure to a combination of stresses on fruit yield and quality is scarce. This work looks at the effect of a long-term combination of salinity and high temperature stresses on tomato yield and fruit quality. Salinity decreased yield but had positive effects on fruit quality, increasing TSS, acidity, glucose, fructose and flavonols. High temperatures increased the vitamin C content but significantly decreased the concentration of some phenolic compounds (hydroxycinnamic acids and flavanones) and some carotenoids (phytoene, phytofluene and violaxanthin). An idiosyncrasy was observed in the effect of a combination of stresses on the content of homovanillic acid O-hexoside, lycopene and lutein, being different than the effect of salinity or high temperature when applied separately. The effect of a combination of stresses may differ from the effects of a single stress, underlining the importance of studying how stress interactions may affect the yield and quality of crops. The results show the viability of exploiting abiotic stresses and their combination to obtain tomatoes with increased levels of health-promoting compounds.
Fluid Dynamic Approaches for Prediction of Spray Drift from Ground Pesticide Applications: A Review
Authors: Hong Se-woon , Park Jinseon , Jeong Hanna , Lee Seyeon , Choi Lakyeong , Zhao Lingying , Zhu Heping
Published: June 2021

Spray drifts have been studied by mathematical models and computer simulations as an essential complement to lab and field tests, among which are fluid dynamic approaches that help to understand the transport of spray droplets in a turbulent atmosphere and their potential impacts to the environment. From earlier fluid mechanical models to highly computational models, scientific advancement has led to a more realistic prediction of spray drift, but the current literature lacks reviews showing the trends and limitations of the existing approaches. This paper is to review the literature on fluid-mechanical-based modelling of spray drift resulting from ground spray applications. Consequently, it provides comprehensive understanding of the transition and development of fluid dynamic approaches and the future directions in this research field.
Determining the Effect of Planting Date and Land Preparation Method on Seedling Emergence, Forage Mass, and Forage Nutritive Value of Forage Brassica
Authors: Denman Tayler D. , Hancock Dennis W. , Dillard S. Leanne , Basinger Nicholas T. , Hale John D.
Published: June 2021

Forage brassicas are used as a forage in monoculture and multispecies mixtures, yet research defining best management practices is limited. Two studies were conducted to evaluate the effects of land preparation method and planting date on establishment, forage mass, and quality of two Brassica species, ‘Inspiration’ canola (Brassica napus L.) and ‘T-Raptor’ hybrid turnip (B. rapa subsp. rapa × B. rapa L.). Each experiment was a randomized complete block design evaluating four land preparation methods: (conventional till (CT), no-till after burning (NB), no-till after mowing (NM), and no-till without residue removal (NR)), and four planting dates: (1 September (S1), 15 September (S15), 1 October (O1), and 15 October (O15)). Significant differences were observed for the canola seedling emergence, but are not of biological significance. Hybrid turnip seedling emergence was the greatest for the CT treatment and consistently greater than NM and NR treatments. Forage mass in the canola study was greatest for the CT and NB. Hybrid turnip forage mass demonstrated similar trends to the canola. CT had the greatest mass and the NR and NM treatments tended to provide the lowest forage mass. Across both studies, earlier planting dates (S1 and S15) resulted in greater overall forage mass than the later planting dates (O1 and O15). Forage nutritive value overall was lower in earlier planted brassica; however, forage nutritive value was not reduced enough to negatively impact livestock.
The Influence of Chicken Egg Lysozyme or Zinc-Bacitracin Antibiotic on the Growth Performance, Antibacterial Capacity, Blood Profiles, and Antioxidative Status of Rabbits: A Comparative Study
Authors: EL-Deep Mahmoud H. , Amber Khairy A. , Eid Yahia Z. , Aboelenin Salama Mostafa , Soliman Mohamed Mohamed , Sakr Mohamed S. , Dawood Mahmoud A. O.
Published: June 2021

Dietary egg lysozyme has beneficial roles in the growth performance and health conditions of animals. The study was performed using 90 multicolored rabbits in three groups (each replicate with thirty rabbits). In the control group, rabbits were fed a diet without zinc bacitracin (ZnB) or egg lysozyme, while the second and third groups were treated with ZnB and lysozyme additive at 100 mg/kg, respectively. After eight weeks, the final weight and body weight gain (BWG) of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p < 0.05). Nevertheless, the feed conversion ratio (FCR) was markedly decreased by dietary egg lysozyme and ZnB (p < 0.05). Interestingly, dietary egg lysozyme resulted in higher final weight and BWG and lower FCR than rabbits treated with ZnB (p < 0.05). Rabbits treated with egg lysozyme and ZnB additives had markedly lower populations of Clostridium spp. and Escherichia coli (p < 0.05) compared with the control. However, the counts of Lactobacillus and total bacteria were meaningfully increased in the the intestines of rabbits treated with egg lysozyme and ZnB (p < 0.05). The blood total protein and globulin of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p < 0.05). Blood creatinine was significantly lowered by dietary egg lysozyme compared with the control and ZnB-treated rabbits (p < 0.05). The levels of blood urea, ALT, and AST were markedly lowered (p < 0.05) by dietary egg lysozyme and ZnB. The gene expressions of superoxide dismutase 1 (SOD1) and glutathione peroxidase (GPX) in the liver of rabbits fed dietary egg lysozyme and ZnB additives were markedly upregulated (p < 0.05) compared with the control. Dietary egg lysozyme resulted in higher expression of SOD1 and GPX genes than rabbits treated with ZnB (p < 0.05). In conclusion, the inclusion of egg lysozyme could replace the inclusion of ZnB in the diets of rabbits.
Diversity of Epibionts Associated with Lepidochelys olivacea (Eschscholtz 1829) Sea Turtles Nesting in the Mexican South Pacific
Authors: Ramos-Rivera Brenda Sarahí , Castro-Mondragon Himmer , Kuk-Dzul José Gabriel , Flores-Rodríguez Pedro , Flores-Garza Rafael
Published: June 2021

The present study contributes to the knowledge of epibionts recorded on sea turtles that nested on a beach in the South Pacific of Mexico. A total of 125 Lepidochelys olivacea turtles nested on Llano Real beach, Guerrero, Mexico, were examined. We collected 450 conspicuous organisms from 8 species from 43 turtles. The corresponding data analysis was carried out to obtain the relative abundance, the relationship between turtle sizes and the presence of organisms, the similarity of species between the sampling months, and the interspecific relationships between the epibionts and the turtles observed. Chelonibia testudinaria was the most abundant species, while Remora remora was the least abundant species. The turtles were divided into six body sections, with the greatest abundance of these organisms located in the head–neck section of turtles, and there was a significant difference in the size of the turtles that presented epibionts and those that did not. C. testudinaria showed greater similarity between sampling months, and the interspecific relationships recorded were commensalism, parasitism, amensalism, and protocooperation. This research contributes the first record of epibionts in L. olivacea nesting in Guerrero, Mexico.
The Return of Large Carnivores and Extensive Farming Systems: A Review of Stakeholders’ Perception at an EU Level
Authors: Franchini Marcello , Corazzin Mirco , Bovolenta Stefano , Filacorda Stefano
Published: June 2021

Conflicts between large carnivores and human activities undermine both the maintenance of livestock practices as well as the conservation of carnivores across Europe. Because large carnivore management is driven by a common EU policy, the purpose of this research was to assess stakeholders’ perception towards bears and wolves at an EU level. We conducted a systematic search and subsequent analysis of 40 peer-reviewed studies collected from 1990 to September 2020 within Member States of the EU. Rural inhabitants and hunters exhibited the most negative attitude compared to urban inhabitants and conservationists, whose attitude was more positive. We showed that direct experience with predators as a consequence of ongoing re-colonization may have affected the degree of acceptance of certain categories and that the long-term coexistence between humans and carnivores does not necessarily imply increased tolerance. To encourage coexistence, we recommend monitoring changes in attitudes over time relative to carnivore population dynamics.
Milk Lactose as a Biomarker of Subclinical Mastitis in Dairy Cows
Authors: Antanaitis Ramūnas , Juozaitienė Vida , Jonike Vesta , Baumgartner Walter , Paulauskas Algimantas
Published: June 2021

Bovine subclinical mastitis can cause great harm to dairy herds because of its negative impact on milk production and quality and cow health. Improved diagnostic tools are needed to maximise the control of subclinical mastitis distribution and ensure the high quality of milk as an industrial product. Between 2015 and 2020, seventy-two dairy herds were screened for bovine subclinical mastitis causative agents to identify the relationship between seasons, lactose levels and subclinical mastitis infection. The predominant species found in the milk samples were mixed microbiota, coagulase-negative Staphylococcus and Staphylococcus aureus. Yeasts were found exclusively in autumn, while Enterococcus spp. and Escherichia coli were only found in summer and autumn. A negative correlation was detected between milk lactose and number of somatic cells in milk (−0.471; p < 0.001). The lactose levels in milk were closely associated with the prevalence (%) of subclinical mastitis pathogens, such as Streptococcus agalactiae (y = −1.8011x + 10.867, R2 = 0.9298), Staph. aureus (y = −3.5216x + 25.957, R2 = 0.8604) and other Streptococci (y = −0.5956x + 7.6179, R2 = 0.6656). These findings suggest that milk lactose may be used as a biomarker of suspected udder inflammation in modern health prevention programmes for cows to reduce the prevalence of subclinical mastitis pathogens in dairy cattle herds.
Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical, and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens
Authors: Hashem Mohamed A. , Abd El Hamied Sahar S. , Ahmed Eman M. A. , Amer Shimaa A. , Hassan Aziza M.
Published: June 2021

The current investigation evaluated the alleviating effects of vitamin C and vitamin E on oxidative stress, hematobiochemical, and histopathological changes in the kidney induced by copper sulfate (CuSO4) toxicity in chickens. Two hundred and fifty-one-day-old male broiler chicks were randomly allotted into five experimental groups (five replicates/group, ten chicks/replicate): 1st group—basal diet with no additives (control group), 2nd group—basal diet complemented with CuSO4 (300 mg/kg diet), 3rd group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet), 4th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin E (250 mg/kg diet), and 5th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet) + vitamin E (250 mg/kg diet) for a 42 day feeding period. The results showed a significant reduction in red blood cells (RBCs), hemoglobin (Hb) concentration, and hematocrit values as well as total leukocyte counts (WBCs), lymphocyte, heterophil, and monocyte counts in the CuSO4-intoxicated birds (2.42 × 106/µL, 9.54 g/dL, 26.02%, 15.80 × 103/µL, 7.86 × 103/µL, 5.26 × 103/µL, and 1.18 × 103/µL, respectively, at the 6th week) compared to (2.79 × 106/µL, 10.98 g/dL, 28.46%, 21.07 × 103/µL, 10.84 × 103/µL, 7.12 × 103/µL, and 1.60 × 103/µL, respectively) in the control group. Moreover, CuSO4-intoxicated birds showed hypoglycemia with a rise in serum uric acid and creatinine levels (122.68, 5.18, and 0.78 mg/dL at the 6th week) compared to (159.46, 4.41, and 0.61 mg/dL) in the control group. The CuSO4 toxicity in birds induced oxidative stress, indicated by a high serum malondialdehyde level (MDA) and diminished activity of the antioxidant enzymes (glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)) (2.01 nmol/mL, 37.66 U/mL, and 2.91 U/mL, respectively, at the 6th week) compared to (1.34 nmol/mL, 57.00 U/mL, 4.99 U/mL, respectively) in the control group. High doses of Cu exposure caused severe microscopic alterations in kidney architecture. The addition of vitamins C and E, singularly or in combination, displayed a beneficial effect in alleviating these harmful effects of Cu toxicity. These findings showed the possible mitigating impacts of dietary antioxidants on the hematobiochemical alterations, oxidative stress, and kidney damage induced by CuSO4 toxicity.
Amino Acid Absorption Profiles in Growing Pigs Fed Different Protein Sources
Authors: Nørgaard Jan V. , Florescu Iulia C. , Krogh Uffe , Nielsen Tina Skau
Published: June 2021

The aim of the present study was to determine postprandial amino acid (AA) appearance in the blood of growing pigs as influenced by protein source. Seven growing pigs (average body weight 18 kg), in a 7 × 5 Youden square design, were fitted with a jugular vein catheter and fed seven diets containing wheat, soybean meal, enzyme-treated soybean meal, hydrothermally-treated rapeseed meal, casein, hydrolyzed casein, and a crystalline AA blend with the same AA profile as casein. The latter was not eaten by the pigs, therefore being excluded. Blood samples were collected at −30, 30, 60, 90, 120, 180, and 360 min after a meal and analyzed for free AA. Overall, plasma AA concentrations were highest 60 min after feeding. There were no differences in plasma AA concentration between casein and hydrolyzed casein, but soybean meal resulted in lower AA plasma concentrations compared with enzyme-treated soybean meal at 60 and 120 min after feeding. There were no differences between hydrothermally-treated rapeseed meal and soybean meal. In conclusion, the ingredients could not clearly be categorized as being slow or fast protein with regard to protein digestion and absorption of AA, but soybean meal resulted in a prolonged appearance of plasma AA compared to casein and hydrolyzed casein.
Effects of Dog-Assisted Education on Physical and Communicative Skills in Children with Severe and Multiple Disabilities: A Pilot Study
Authors: Lobato Rincón Luis Lucio , Rivera Martín Beatriz , Medina Sánchez María Ángeles , Villafaina Santos , Merellano-Navarro Eugenio , Collado-Mateo Daniel
Published: June 2021

Animal-assisted interventions have shown promising benefits in different populations such as children with cerebral palsy or autism spectrum disorder. Human–animal interaction leads to different physical, cognitive, and emotional benefits in the child. The aim of the current pilot study was to evaluate the effects of a dog-assisted education program on the postural, oculomotor, linguistic and autonomy dimensions in children affected by severe and multiple disabilities. Fourteen children aged 3–12 years and affected by intellectual and physical disabilities participated in a dog-assisted program consisted of 12 sessions. The intervention involved different types of activities, exercises, and games with the dogs. A strict protocol to ensure animal wellbeing and avoid any type of stress or fatigue was followed. Children who participated in the study improved their postural control, eye-motor coordination, expression of sensations and feelings, spontaneous interaction, autonomy, and confidence. However, these results must be taken with caution due to the lack of a control group and the heterogeneity of the participants.
Elucidating the Antimycobacterial Mechanism of Action of Decoquinate Derivative RMB041 Using Metabolomics
Authors: Knoll Kirsten E. , Lindeque Zander , Adeniji Adetomiwa A. , Oosthuizen Carel B. , Lall Namrita , Loots Du Toit
Published: June 2021

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), still remains one of the leading causes of death from a single infectious agent worldwide. The high prevalence of this disease is mostly ascribed to the rapid development of drug resistance to the current anti-TB drugs, exacerbated by lack of patient adherence due to drug toxicity. The aforementioned highlights the urgent need for new anti-TB compounds with different antimycobacterial mechanisms of action to those currently being used. An N-alkyl quinolone; decoquinate derivative RMB041, has recently shown promising antimicrobial activity against Mtb, while also exhibiting low cytotoxicity and excellent pharmacokinetic characteristics. Its exact mechanism of action, however, is still unknown. Considering this, we used GCxGC-TOFMS and well described metabolomic approaches to analyze and compare the metabolic alterations of Mtb treated with decoquinate derivative RMB041 by comparison to non-treated Mtb controls. The most significantly altered pathways in Mtb treated with this drug include fatty acid metabolism, amino acid metabolism, glycerol metabolism, and the urea cycle. These changes support previous findings suggesting this drug acts primarily on the cell wall and secondarily on the DNA metabolism of Mtb. Additionally, we identified metabolic changes suggesting inhibition of protein synthesis and a state of dormancy.
Super-Cationic Peptide Dendrimers—Synthesis and Evaluation as Antimicrobial Agents
Authors: Ramchuran Estelle J. , Pérez-Guillén Isabel , Bester Linda A. , Khan René , Albericio Fernando , Viñas Miguel , de la Torre Beatriz G.
Published: June 2021

Microbial infections are a major public health concern. Antimicrobial peptides (AMPs) have been demonstrated to be a plausible alternative to the current arsenal of drugs that has become inefficient due to multidrug resistance. Herein we describe a new AMP family, namely the super-cationic peptide dendrimers (SCPDs). Although all members of the series exert some antibacterial activity, we propose that special attention should be given to (KLK)2KLLKLL-NH2 (G1KLK-L2KL2), which shows selectivity for Gram-negative bacteria and virtually no cytotoxicity in HepG2 and HEK293. These results reinforce the validity of the SCPD family as a valuable class of AMP and support G1KLK-L2KL2 as a strong lead candidate for the future development of an antibacterial agent against Gram-negative bacteria.
The Association between the Risk of Aortic Aneurysm/Aortic Dissection and the Use of Fluroquinolones: A Systematic Review and Meta-Analysis
Authors: Lai Chih-Cheng , Wang Ya-Hui , Chen Kuang-Hung , Chen Chao-Hsien , Wang Cheng-Yi
Published: June 2021

This study aimed to investigate the association between the risk of aortic aneurysm (AA)/aortic dissection (AD) and the use of fluoroquinolones (FQs). PubMed, Embase, Cochrane CENTRAL, Cochrane Database of Systematic Reviews, Web of Science and Scopus were searched for relevant articles to 21st February 2021. Studies that compared the risk of AA/AD in patients who did and did not receive FQs or other comparators were included. The pooled results of nine studies with 11 study cohorts showed that the use of FQs increased the risk of AA/AD by 69% (pooled risk ratio (RR) = 1.69 (95% CI = 1.08, 2.64)). This significant association remained unchanged using leave-one-out sensitivity test analysis. Similar results were found for AA (pooled RR = 1.58 (1.21, 2.07)) but no significant association was observed for AD (pooled RR = 1.23 (0.93, 1.62)). Stratified by the comparators, the use of FQs was associated with a significantly higher risk of AA/AD compared to azithromycin (pooled RR = 2.31 (1.54, 3.47)) and amoxicillin (pooled RR = 1.57 (1.39, 1.78)). In contrast, FQ was not associated with a higher risk of AA/AD, when compared with amoxicillin/clavulanic acid or ampicillin/sulbactam (pooled RR = 1.18 (0.81, 1.73)), sulfamethoxazole–trimethoprim (pooled RR = 0.89 (0.65, 1.22)) and other antibiotics (pooled RR = 1.14 (0.90, 1.46)). In conclusion, FQs were associated with an increased risk of AA or AD, although the level of evidence was not robust. However, FQs did not exhibit a higher risk of AA or AD compared with other broad-spectrum antibiotics. Further studies are warranted to clarify the role of FQs in the development of AA or AD.
How Will Rwandan Land Use/Land Cover Change under High Population Pressure and Changing Climate?
Authors: Li Chaodong , Yang Mingyi , Li Zhanbin , Wang Baiqun
Published: June 2021

In recent decades, population growth and economic development have greatly influenced the pattern of land use/land cover (LULC) in Rwanda. Nevertheless, LULC patterns and their underlying change mechanisms under future climate conditions are not well known. Therefore, it is particularly important to explore the direction of LULC transfer in the study area, identify the factors driving the transfer of different types of LULC and their changes, and simulate future LULC patterns under future climate conditions. Based on LULC analyses of Rwanda in 1990, 2000, 2010, and 2015, the LULC pattern of Rwanda in the next 30 years was simulated using an LULC transition matrix, random forest sampling, the Markov chain model, and the PLUS model. The results showed that LULC change in the study area primarily comprised a decrease in forest area and expansion of cropland area, accompanied by a small increase in grassland area and an annual increase in urban land area. Prior to 2000, the LULC in Rwanda was mainly converted from forest and grassland to cropland, with the ratio being 0.72:0.28. After 2010, the LULC was mainly converted from forest to grassland and cropland, with the ratio being 0.83:0.17. Changes in forests, grasslands, and cropland are driven by multiple factors, whereas changes in wetlands, water, urban land, and unused land are more likely to be driven by a single factor. The existing trend of LULC change will continue for the next 30 years, and the future LULC pattern will exhibit a trend in which cropland area will increase in the west and grassland area will decrease, whereas grassland area will increase in the east and cropland area will decrease.
Dynamic Analysis and Experiment of a Space Mirror Based on a Linear State Space Expression
Authors: Liu Ruijing , Li Zongxuan , Xu Wei , Yang Xiubin , Zhang Defu , Yao Zhao , Yang Kun
Published: June 2021

Dynamic analysis of the optical–mechanical structure based on the linear state space expression is performed for a three-point flexural mount lightweight space mirror with a diameter of ϕ740 mm. Using linear structure dynamics and linear state space theories, the state space model of the mirror assembly is established based on modal information. The DC gain method is used to reduce modes and a frequency response analysis of the reduced modes is performed to obtain the frequency domain transfer function between the excitation input and response output points and determine the contribution of each mode to the total frequency response. The frequency response curve is plotted. A mechanical vibration test is performed to verify the accuracy and rationality of simulation analysis. The dynamic analysis method based on state space theory provides a new method of investigating optical and mechanical structures, which can help efficiently and accurately analyze the frequency response characteristics of complex linear systems.
An Approach for Detecting Feasible Paths Based on Minimal SSA Representation and Symbolic Execution
Authors: Marashdih Abdalla Wasef , Zaaba Zarul Fitri , Suwais Khaled
Published: June 2021

Static analysis is one of the techniques used today to analyze source codes and minimize the issue of software vulnerability. Static analysis has the ability to observe all possible software paths in an application through the scrutiny of a web application’s source code. Among those paths, some may be considered feasible paths, which refer to any paths that the test cases can execute. The detection of feasible paths in the results of a static analysis helps to minimize the false positive rate. However, the detection of feasible paths can be challenging, especially for programs that have multiple conditions in the same branch. The aim is to ensure that each feasible path is detected only once (not duplicated). This paper proposes an approach based on minimal static single assignment (MSSA) form and symbolic execution to detect feasible paths. The proposed approach starts by converting the source code into an abstract syntax tree (AST), followed by converting the AST to minimal SSA representation, which helps to decrease the number of instructions in the SSA form. An algorithm was built to examine all of the instructions of the SSA form, identify whole paths in the source code, and extract constraints along each path. A path weight method (PWM) is proposed in this work to avoid detecting duplicated feasible paths. The satisfiability modulo theory (SMT) solver was used to check the satisfiability of each path condition. The proposed approach was tested on seven well-known test programs that have been used in related studies and 10 large scale programs. The experimental results indicate that the proposed method (PWM) can avoid detecting duplicated feasible paths, and the proposed approach reduced the time required for generating the paths compared to that in related studies.
A Novel Advancing Signal Processing Method Based on Coupled Multi-Stable Stochastic Resonance for Fault Detection
Authors: Cui Hongjiang , Guan Ying , Chen Huayue , Deng Wu
Published: June 2021

In recent years, methods for detecting motor bearing faults have attracted increasing attention. However, it is very difficult to detect the faults from weak motor bearing signals under the strong noise. Stochastic resonance (SR) is a popular signal processing method, which can process weak signals with the noise, but the traditional SR is burdensome in determining its parameters. Therefore, in this paper, a new advancing coupled multi-stable stochastic resonance method, with two first-order multi-stable stochastic resonance systems, namely CMSR, is proposed to detect motor bearing faults. Firstly, the effects of the output signal-to-noise ratio (SNR) for system parameters and coupling coefficients are analyzed in-depth by numerical simulation technology. Then, the SNR is considered as the fitness function for the seeker optimization algorithm (SOA), which can adaptively optimize and determine the system parameters of the SR by using the subsampling technique. An advancing coupled multi-stable stochastic resonance method is realized, and the pre-processed signal is input into the CMSR to detect the faults of motor bearings by using Fourier transform. The faults of motor bearings are determined according to the output signal. Finally, the actual vibration data of induction motor bearings are used to prove the effectiveness of the proposed CMSR. The comparison results with the MSR show that the CMSR can obtain a higher output SNR, which is more beneficial to extract weak signal features and realize fault detection. At the same time, this method also has practical application value for engineering rotating machinery.
Complete Characterization of Degradation Byproducts of Olmesartan Acid, Degradation Pathway, and Ecotoxicity Assessment
Authors: Luongo Giovanni , Siciliano Antonietta , Libralato Giovanni , Guida Marco , Saviano Lorenzo , Previtera Lucio , Di Fabio Giovanni , Zarrelli Armando
Published: June 2021

Antihypertensive drugs are among the most prescribed drugs. Olmesartan acid, of the sartan class, belongs to a relatively new generation of antihypertensive drugs called angiotensin II receptor blockers. There are very few studies on the presence and fate of sartans in the environment, despite them being marketed in huge quantities, metabolized in low percentages, and detected in wastewater and water bodies. This paper presents a study on the less abundant and more polar fractions that have been neglected in previous studies, which led to the isolation by chromatographic methods of thirteen degradation byproducts (DPs), six of which are new, identified by nuclear magnetic resonance and mass spectrometry. A mechanism of degradation from the parent drug was proposed. The ecotoxicity of olmesartan acid and identified compounds was evaluated in Aliivibrio fischeri bacteria and Raphidocelis subcapitata algae to assess acute and chronic toxicity. For 75% of the DPs, acute and chronic exposure to the compounds, at concentrations of 5 mg/L, inhibited population growth in the algae and decreased bioluminescence in the bacteria.
Melting of Paraffin Waxes Embedded in a Porous Matrix Made by Additive Manufacturing
Authors: Diani Andrea , Moro Lorenzo , Rossetto Luisa
Published: June 2021

The recent advances in additive manufacturing technology have widened the choice of materials that can be printed, opening new frontiers in the field of heat transfer devices. This paper explores the use of a solid porous matrix in which paraffin waxes, having different melting temperatures (42, 55, and 64 °C), were embedded. The solid matrix is made by additive manufacturing. The parent cell of the porous matrix occupies the volume of a cube with an edge of 5 mm. The entire 3D printed matrix has a square base with an edge of 100 mm, and it has a height of 20 mm. The solid matrix was printed between two plates, each one with a thickness of 10 mm, where thermocouples were inserted, and it was tested in an upright position, laterally heated applying three different heat fluxes (10, 15, and 20 kW m−2). The experimental results are given in terms of the temperature of the heated side, as well as of the phase change material, during the heating process. The temperature reached by the heated side and the time needed to completely melt the paraffin waxes are compared at the different working conditions. Furthermore, the thermal conductivities and diffusivities of the three paraffins and of the parent material of the porous matrix were experimentally evaluated.
BIM-Based Research Framework for Sustainable Building Projects: A Strategy for Mitigating BIM Implementation Barriers
Authors: Manzoor Bilal , Othman Idris , Gardezi Syed Shujaa Safdar , Altan Haşim , Abdalla Salem Buhashima
Published: June 2021

Although Building Information Modeling (BIM) can enhance efficiency of sustainable building projects, its adoption is still plagued with barriers. In order to incorporate BIM more efficiently, it is important to consider and mitigate these barriers. The aim of this study is to explore and develop strategies to alleviate barriers in developing countries, such as Malaysia, to broaden implementation of BIM with the aid of quantitative and qualitative approaches. To achieve this aim, a comprehensive literature review was carried out to identify the barriers, and a questionnaire survey was conducted with construction projects’ stakeholders. The ranking analysis results revealed the top five critical barriers to be “unavailability of standards and guidelines”, “lack of BIM training”, “lack of expertise”, “high cost”, and “lack of research and BIM implementation”. Comparative study findings showed that “lack of research and BIM implementation” is the least important barrier in other countries like China, United Kingdom, Nigeria, and Pakistan. Furthermore, qualitative analysis revealed the strategies to mitigate the BIM implementation barriers to enhance sustainable goals. The final outcome of this study is the establishment of a framework incorporated with BIM implementation barriers and strategies namely, the “BIM-based research framework”, which can assist project managers and policymakers towards effective sustainable construction.
Evaluation of Weakening Characteristics of Reinforced Concrete Using High-Frequency Induction Heating Method
Authors: Lim Myung-kwan , Lee Changhee
Published: June 2021

To increase the quality of recycling, a new demolition technique is required that can work in parallel with existing crushing methods, which use large equipment with high crushing efficiency. Moreover, the efficient collection of the remains from the fractional dismantling method needs to be considered based on its procedure, and the technology for partial dismantling that is efficient in remodeling, maintenance, and reinforcement has to be developed. In this study, the temperature-increasing characteristics of rebars inside ferroconcrete with respect to their arrangement was investigated by partial rapid heating through high-frequency induction heating. Based on this, the chemical and physical vulnerability characteristics of ferroconcrete due to the thermal conduction generated on the rebar surface and the cracks caused by the thermal expansion pressure of the rebar were verified. In addition, the objective of this study was to verify the applicability of the technology by specifying the vulnerability range of ferroconcrete based on the heating range with adequate consumption of energy.
Synergistic Effects of Heat-Moisture Treatment Regime and Grape Peels Addition on Wheat Dough and Pasta Features
Authors: Iuga Mădălina , Batariuc Ana , Mironeasa Silvia
Published: June 2021

Heat moisture treatment (HMT) can be a useful method of wheat flour functionality modification, improving the nutritional value of pasta along with grape peels (GPF) addition. The aim of this study was to investigate the combined effects of HMT temperature, time, and moisture and GPF level on dough and pasta properties. Dough rheology and texture, pasta color, texture, total polyphenols (TPC), dietary fiber (DF), and resistant starch (RS) contents were evaluated. Furthermore, an optimization was performed based on Response Surface Methodology (RSM) and desirability function. The results showed that HMT regime and GPF determined proportional dough viscoelastic moduli and firmness increase. On the other hand, cooked pasta firmness and gumminess decreased with HMT conditions and GPF level rise. Higher pasta RS and DF content was promoted by HMT and GPF components. The reduction effect of HMT on TPC was countered by the incorporation of GPF, a rich source of polyphenols. The optimization revealed that the recommended wheat flour treatment regime would be 87.56 °C, 3 h, and 26.01% moisture, while the quantity of GPF that could be added was 4.81%. For these values, the maximum functional and nutritional values would be achieved with minimum negative impact on pasta quality.