bluuebunny/crossref_metadata_2025 huggingface dataset:
Modified Hemocyanins from Rapana thomasiana and Helix aspersa Exhibit Strong Antitumor Activity in the B16F10 Mouse Melanoma Model
Authors: Stoyanova Emiliya , Mihaylova Nikolina , Ralchev Nikola , Bradyanova Silviya , Manoylov Iliyan , Raynova Yuliana , Idakieva Krassimira , Tchorbanov Andrey
Published: October 2024

Melanoma is one of the most common tumors worldwide, and new approaches and antitumor drugs for therapy are being investigated. Among the promising biomolecules of natural origin for antitumor research are gastropodan hemocyanins—highly immunogenic multimeric glycoproteins used as antitumor agents and components of therapeutic vaccines in human and mouse cancer models. A murine melanoma model established in C57BL/6 mice of the B16F10 cell line was used to study anticancer modified oxidized hemocyanins (Ox-Hcs) that were administered to experimental animals (100 μg/mouse) under different regimens: mild, intensive, and with sensitization. The solid tumor growth, antitumor response, cell infiltration in tumors, and survival were assessed using flow cytometry, ELISA, and cytotoxicity assays. Therapy with Ox-RtH or Ox-HaH resulted in the generation of enhanced specific immune response (increased levels of tumor-infiltrated mature NK cells (CD27+CD11b+) in sensitized groups and of macrophages in the intensively immunized animals) and tumor suppression. Beneficial effects such as delayed tumor incidence and growth as well as prolonged survival of tumor-bearing animals have been observed. High levels of melanoma-specific CTLs that mediate cytotoxic effects on tumor cells; tumor-infiltrating IgM antibodies expected to enhance antibody-dependent cellular cytotoxicity; type M1 macrophages, which stimulate the Th1 response and cytotoxic cells; and proinflammatory cytokines, were also observed after Ox-Hcs administration. The modified Hcs showed strong antitumor properties in different administration regimens in a murine model of melanoma with potential for future application in humans.
Current Challenges and Future Prospects in Human Reproduction and Infertility
Authors: Henry Laurie , Nisolle Michelle
Published: October 2024

Human reproduction is a captivating yet intricate field, constantly presenting new challenges and discoveries [...]
Oral and Pharyngeal Diverticula: A Rare Case of Dysphagia and Diagnostic Challenges
Authors: Alfaris Abdullah Mohammed , Al Awaji Nisreen Naser , Alabdulkarim Shaden Abdulmohsen , Mallisho Ammar Mammoun , Hamdoun Anas Osman , Bawazir Samir Mohammed , AlAjroush Noura Ahmed
Published: October 2024

This report describes the case of a 62-year-old male patient in Saudi Arabia who developed a diverticular pouch in his oral cavity. Diverticula are rare conditions that appear as protrusions or pouches within the digestive tract’s inner lining. The condition can occur in different parts of the digestive system, but the colon is the most affected part. As part of the patient’s symptoms, he presented with dysphagia, weight loss, and globus sensations. Multiple diagnostic tests, including ultrasound, computerized tomography (CT), and magnetic resonance imaging (MRI), did not identify the diverticula. Barium and upper gastrointestinal studies were the diagnostic tests that provided accurate diagnoses where several diverticula were discovered during the videofluoroscopic swallow study (VFSS) and fiberoptic endoscopic evaluation of swallowing (FEES). Surgical intervention was recommended by the multidisciplinary team; however, the patient rejected this treatment option. This report highlights the necessity for instrumental swallowing diagnostic evaluation in the workup of the infrequent etiologies of dysphagia and suggests a gap in the current knowledge, prompting further studies on the etiology, incidence, and optimum management of upper gastrointestinal (GI) tract diverticular disease.
Unveiling the Value of Meta-Analysis in Disease Prevention and Control: A Comprehensive Review
Authors: Ntais Christos , Talias Michael A.
Published: October 2024

Given the plethora of studies today that focus on the same topic, clinicians and other healthcare professionals increasingly rely on meta-analysis to aid in their evidence-based decision-making. This research method, which combines data from multiple studies to produce a single, more precise estimate of effect size, is invaluable for synthesizing evidence, resolving inconsistencies and guiding clinical practice and public health policies. Especially in disease prevention and control, meta-analysis has emerged as a critical tool. Meta-analysis is particularly valuable in assessing the effectiveness of preventive interventions such as vaccines, lifestyle modifications and screening programs. It provides robust evidence that supports the implementation of effective preventive measures and the discontinuation of ineffective or harmful ones. Furthermore, meta-analysis provides evidence to develop clinical practice guidelines, ensuring patients receive evidence-based treatments. In addition, public health policies aimed at disease prevention and control often rely on evidence from meta-analyses, which provide the data needed to justify and design large-scale public health initiatives. This comprehensive review delves into the role of meta-analysis in disease prevention and control, exploring its advantages, applications, challenges and overall impact on guiding clinical practice and public health policies. Through case studies and an examination of future directions, this paper underscores the pivotal role of meta-analysis in disease prevention and control.
Superior Biological Healing of Hamstring Autografts Compared with Tibialis Allografts after Anterior Cruciate Ligament Reconstruction: A Propensity Score Matching Analysis Based on Second-Look Arthroscopy
Authors: Lee Seo-Jun , Park Jun-Gu , Han Seung-Beom , Bae Ji-Hoon , Jang Ki-Mo
Published: October 2024

Background and Objectives: Remodeling and healing of the graft are crucial processes for long-term graft survival after anterior cruciate ligament reconstruction (ACLR). However, few studies have objectively evaluated the differences in graft healing between autografts and allografts. This study aimed to compare the status of the anterior cruciate ligament (ACL) grafts between hamstring tendon (HT) autografts and tibialis anterior tendon (TAT) allografts using second-look arthroscopy. Materials and Methods: The outcomes of 193 consecutive patients (153 males and 40 females, with an average age of 30.38 and BMI of 25.43 kg/m2) who underwent second-look arthroscopy following primary ACLR were retrospectively reviewed. Prior to participating in this study, all patients provided written informed consent. The patients were divided into two groups: those with HT autografts and those with TAT allografts. Confounding factors were matched between the two groups using propensity score matching (PSM). ACL graft status was assessed during second-look arthroscopy using a numeric scale system based on the degree of four parameters: graft tension, continuity, synovium coverage, and vascular marking. Clinical outcomes were assessed using the Lysholm and International Knee Documentation Committee (IKDC) scores. Graft status and clinical outcomes were compared between the two groups. Additionally, a subgroup analysis based on the timing of the second-look arthroscopy (12–24 months vs. >24 months after the initial ACLR) was conducted. Results: After PSM, 62 patients were included in each group. The second-look arthroscopy was conducted at 23.6 ± 6.6 months for the HT group and at 24.0 ± 7.9 months for the TAT group (p = 0.749). The continuity and tension of the ACL graft were not significantly different between the two groups (p = 0.146 and 0.075, respectively). However, the TAT group exhibited significantly inferior synovial coverage and vascular marking of the ACL graft compared with the HT group (p = 0.021 and 0.007, respectively). These findings were consistent regardless of the timing of the second-look arthroscopy. Clinical outcomes, according to the Lysholm and IKDC scores, significantly improved in both groups with no significant differences (p = 0.386 and 0.733, respectively). Conclusions: Although there were no differences in graft tension and continuity between HT autografts and TAT allografts, the biological healing of ACL grafts, in terms of synovialization and vascularization, was superior in HT autografts compared to TAT allografts.
A Potential Pneumothorax Induced by Immune Checkpoint Inhibitors: A Case Report and Literature Review
Authors: Shin Yoon-E , Kim Hyuk , Yoo Jeong-Ju , Kim Sang Gyune , Kim Young Seok
Published: October 2024

Background and Objectives: Immune checkpoint inhibitors (ICIs), which target immune checkpoints in cancer cells, are increasingly used as a mainstay in anticancer treatment. The combination of atezolizumab and bevacizumab is also a first-line treatment for hepatocellular carcinoma (HCC). However, ICIs can cause immune-related adverse events (IrAEs) which range from mild to severe, potentially leading to the need for discontinuing immunotherapy. We report a case of a pneumothorax, a rare side effect caused by IrAEs. Materials and Methods: This paper reports a case of a 78-year-old male HCC patient who developed a recurrent pneumothorax, suspected to be an adverse effect of ICIs. Results: The patient was a current smoker with a 30 pack-year smoking history. Prior to initiating ICIs, a chest CT scan showed mild emphysema and fibrosis attributable to smoking. Following ICI treatment, the patient developed a recurrent pneumothorax. Further tests revealed no underlying cause for the pneumothorax other than the ICIs and smoking, and there were no signs of intrapulmonary metastasis or pneumonitis. Conclusions: When a pneumothorax occurs in a patient undergoing immunotherapy, it is important to consider it as a potential adverse effect of the treatment. Special attention should be given to the possibility that immunotherapy may exacerbate underlying lung conditions. Patients should be advised on the importance of smoking cessation. As there are currently no guidelines for resuming immunotherapy after a pneumothorax, it is crucial to weigh the risks and benefits and consider dose reduction or discontinuation of the medication.
The Determination of the Elastoplastic and Phase-Field Parameters for Monotonic and Fatigue Fracture of Sintered Steel Astaloy™ Mo+0.2C
Authors: Polančec Tomislav , Lesičar Tomislav , Tonković Zdenko
Published: October 2024

This paper presents a procedure for determining the elastoplastic parameters of phase-field fracture of sintered material. The material considered was sintered steel Astaloy™ Mo+0.2C of three densities: 6.5, 6.8 and 7.1 g/cm3. The stress–strain curve and Wöhler curve, which are experimentally obtained, are utilized for validation of the numerical simulations. For modelling of damage evolution, a CCPF (Convergence check phase-field) algorithm was used as a numerical framework. During calibration of the numerical parameters, two-dimensional as well as three-dimensional modelling was used. A comparison of different fatigue degradation functions known from the literature is also made. To improve the efficiency of numerical simulations of fatigue behaviour, the cycle skip technique is also employed.
Association between Inflammatory and Metabolic Biomarkers and Common Mental Disorders among Adults: 2015 Health Survey of São Paulo, SP, Brazil
Authors: Ferreira Letícia do Nascimento Maximiano , Fisberg Regina Mara , Sarti Flavia Mori , Rogero Marcelo Macedo
Published: October 2024

Recent studies suggest that plasma inflammatory biomarker concentrations may represent valuable indicators for the diagnosis and prognosis of mental disorders. At the same time, metabolic alterations may contribute to the development and progression of systemic low-grade inflammation. Background/Objectives: This study evaluated the association between plasma inflammatory biomarkers and common mental disorders (CMD), exploring the relationship between metabolic biomarkers, metabolic syndrome (MetS), and inflammatory biomarkers in younger and older adults. Methods: This cross-sectional study used data from the 2015 Health Survey of São Paulo with a Focus on Nutrition Study. The occurrence of CMD was assessed through the Self-Reporting Questionnaire (SRQ-20). Blood samples were used to measure plasma concentrations of inflammatory and cardiometabolic biomarkers. MetS was defined according to the International Diabetes Federation Consensus. The Mann–Whitney test compared inflammatory biomarker concentrations across CMD groups and cardiometabolic conditions, and logistic regression models explored associations between inflammatory biomarker concentration and CMD. Results: The sample included 575 participants, 22.6% (n = 130) of whom had CMD. Concentrations of plasminogen activator inhibitor 1, C-reactive protein (CRP), and the systemic low-grade inflammation score varied significantly among CMD groups. CRP concentrations were positively associated with the presence of CMD, independent of confounding factors. Participants with insulin resistance, dyslipidemia, and MetS exhibited significantly higher CRP concentrations than individuals without these conditions. Conclusions: The findings suggest that increased plasma CRP concentrations may be a potential risk factor for CMD. Higher CRP concentrations were observed in individuals with insulin resistance, dyslipidemia, and MetS. Future interventional studies should explore these hypotheses in diverse populations.
Resistant Potato Starch Supplementation Reduces Serum Free Fatty Acid Levels and Influences Bile Acid Metabolism
Authors: Bush Jason R. , Iwuamadi Izuchukwu , Han Jun , Schibli David J. , Goodlett David R. , Deehan Edward C.
Published: October 2024

Background: Resistant starches, such as high-amylose maize starch and resistant potato starch (RPS), have prebiotic effects that are linked to improved metabolism at >15 g/day, but the effects at lower doses have not been reported. Methods: We performed an exploratory post hoc analysis of free fatty acids (FFAs), bile acids (BAs), and ketone bodies in serum previously collected from a randomized, double-blind, placebo-controlled clinical trial evaluating the effects of one- and four-week consumption of 3.5 g/day RPS versus a placebo using two-way ANOVA adjusted by pFDR. Associations between week 4 changes in FFAs, BAs, and ketone bodies were assessed by Pearson’s correlations. Results: RPS consumption reduced total FFAs relative to the placebo, including multiple unsaturated FFAs and octanedioic acid, with reductions in taurine- and glycine-conjugated secondary BAs also detected (q < 0.05). No changes in ketone bodies were observed (q > 0.05). Changes in 7-ketodeoxycholic acid (r = −0.595) and glycolithocholic acid (r = −0.471) were inversely correlated with treatment-induced reductions in FFAs for RPS but not the placebo, suggesting the effects were from the prebiotic. Shifts in β-hydroxybutyrate were further correlated with FFA changes in both treatments (q < 0.05). Conclusions: These findings demonstrate that low doses of RPS positively influence fatty acid metabolism in humans, reducing circulating levels of FFA and conjugated BAs.
Hydrogen-Rich Water to Enhance Exercise Performance: A Review of Effects and Mechanisms
Authors: Zhou Qiaorui , Li Huixin , Zhang Ye , Zhao Yirui , Wang Can , Liu Chang
Published: October 2024

Background: Hydrogen-rich water (HRW) has garnered significant interest within the sports and exercise science community due to its selective antioxidant properties. Despite its potential benefits, comprehensive reviews specifically addressing its effects on athletic performance are limited. This review aims to assess the impact of HRW on sports performance and explore the underlying molecular biological mechanisms, with the goal of elucidating how HRW might enhance athletic performance. Methods: This review synthesizes research on HRW by examining articles published between 1980 and April 2024 in databases such as PubMed, the Cochrane Library, Embase, Scopus, and Web of Science. Results: It highlights HRW’s effects on various aspects of athletic performance, including endurance, strength, sprint times, lunge movements, countermovement jump height, and time to exhaustion. While the precise mechanisms by which HRW affects athletic performance remain unclear, this review investigates its general molecular biological mechanisms beyond the specific context of sports. This provides a theoretical foundation for future research aimed at understanding how HRW can enhance athletic performance. HRW targets the harmful reactive oxygen and nitrogen species produced during intense exercise, thereby reducing oxidative stress—a critical factor in muscle fatigue, inflammation, and diminished athletic performance. HRW helps to scavenge hydroxyl radicals and peroxynitrite, regulate antioxidant enzymes, mitigate lipid peroxidation, reduce inflammation, protect against mitochondrial dysfunction, and modulate cellular signaling pathways. Conclusions: In summary, while a few studies have indicated that HRW may not produce significant beneficial effects, the majority of research supports the conclusion that HRW may enhance athletic performance across various sports. The potential mechanisms underlying these benefits are thought to involve HRW’s role as a selective antioxidant, its impact on oxidative stress, and its regulation of redox homeostasis. However, the specific molecular biological mechanisms through which HRW improves athletic performance remain to be fully elucidated.
Theoretical Study on Transverse Mode Instability in Raman Fiber Amplifiers Considering Mode Excitation
Authors: Huang Shanmin , Hao Xiulu , Li Haobo , Fan Chenchen , Chen Xiao , Yao Tianfu , Huang Liangjin , Zhou Pu
Published: October 2024

Raman fiber lasers (RFLs), which are based on the stimulated Raman scattering effect, generate laser beams and offer distinct advantages such as flexibility in wavelength, low quantum defects, and absence from photo-darkening. However, as the power of the RFLs increases, heat generation emerges as a critical constraint on further power scaling. This escalating thermal load might result in transverse mode instability (TMI), thereby posing a significant challenge to the development of RFLs. In this work, a static model of the TMI effect in a high-power Raman fiber amplifier based on stimulated thermal Rayleigh scattering is established considering higher-order mode excitation. The variations of TMI threshold power with different seed power levels, fundamental mode purities, higher-order mode losses, and fiber lengths are investigated, while a TMI threshold formula with fundamental mode pumping is derived. This work will enrich the theoretical model of TMI and extend its application scope in TMI mitigation strategies, providing guidance for understanding and suppressing TMI in the RFLs.
Area-Time-Efficient Secure Comb Scalar Multiplication Architecture Based on Recoding
Authors: Zhang Zhantao , Wang Weijiang , Zhang Jingqi , He Xiang , Ma Mingzhi , Ren Shiwei , Dang Hua
Published: October 2024

With the development of mobile communication, digital signatures with low latency, low area, and high security are in increasing demand. Elliptic curve cryptography (ECC) is widely used because of its security and lightweight. Elliptic curve scalar multiplication (ECSM) is the basic arithmetic in ECC. Based on this background information, we propose our own research objectives. In this paper, a low-latency and low-area ECSM architecture based on the comb algorithm is proposed. The detailed methodology is as follows. The recoding-k algorithm and randomization-Z algorithm are used to improve security, which can resist sample power analysis (SPA) and differential power analysis (DPA). A low-area multi-functional architecture for comb is proposed, which takes into account different stages of the comb algorithm. Based on this, the data dependency is considered and the comb architecture is optimized to achieve a uniform and efficient execution pattern. The interleaved modular multiplication algorithm and modified binary inverse algorithm are used to achieve short clock cycle delay and high frequency while taking into account the need for a low area. The proposed architecture has been implemented on Xilinx Virtex-7 series FPGA to perform ECSM on 256-bits prime field GF(p). In the hardware architecture with only 7351 slices of resource usage, a single ECSM only takes 0.74 ms, resulting in an area-time product (ATP) of 5.41. The implementation results show that our design can compete with the existing state-of-the-art engineering in terms of performance and has higher security. Our design is suitable for computing scenarios where security and computing speed are required. The implementation of the overall architecture is of great significance and inspiration to the research community.
Microalgal Metabolomes and Recent Biotechnological Advances for Their Industrial Application
Authors: Saini Dinesh Kumar , Manchanda Geetanjali , Chaudhary Ratiram Gomaji , Singh Raghvendra Pratap
Published: October 2024

In recent decades, microalgae have emerged as new sustainable resources for the production of various bioactive and biochemical compounds. These organisms are photosynthetic, use CO2 as their sole carbon source, and grow rapidly under different environmental conditions. This makes microalgae a promising source of feedstock for many bio-commodities. However, the economic viability for large-scale production through microalgal cells is limited to certain organisms. Recent advances in omics technologies, along with high-throughput approaches, have provided new insights for understanding microalgal metabolites. With the improvement in metabolomic technology, sensitivity for the detection of cellular metabolites has increased, and it has directly enhanced our understanding of cellular metabolism and the corresponding metabolic pathways. Moreover, these metabolic techniques, along with other omics technologies, help us to characterize the changes in the cellular functioning of the different cells under environmental conditions. Metabolomic research on microalgae explores new metabolites and the understanding of their biosynthesis at the metabolic level. In this review, we discuss how these metabolomics techniques are applied to microalgae to study their metabolic networks. Additionally, we also discuss how integrating other tools of systems biology can improve the productivity of microalgal cells, especially for industrially important commodities.
Genomic and Transcriptomic Analyses Identify Two Key Glycosyltransferase Genes alhH and alhK of Exopolysaccharide Biosynthesis in Pantoea alhagi NX-11
Authors: He Kun , Shi Xiaolong , Tao Zhongming , Hu Xing , Sun Liang , Wang Rui , Gu Yian , Xu Hong , Qiu Yibin , Lei Peng
Published: October 2024

The exopolysaccharide (EPS) produced by Pantoea alhagi NX-11, referred to as alhagan, enhances plant stress resistance, improves soil properties, and exhibits notable rheological properties. Despite these benefits, the exact bio-synthetic process of alhagan by P. alhagi NX-11 remains unclear. This study focused on sequencing the complete genome of P. alhagi NX-11 and identifying an alhagan synthesis gene cluster (LQ939_RS12550 to LQ939_RS12700). Gene annotation revealed that alhagan biosynthesis in P. alhagi NX-11 follows the Wzx/Wzy-dependent pathway. Furthermore, transcriptome analysis of P. alhagi NX-11 highlighted significant upregulation of four glycosyltransferase genes (alhH, wcaJ, alhK, and alhM) within the alhagan synthesis gene cluster. These glycosyltransferases are crucial for alhagan synthesis. To delve deeper into this process, two upregulated and uncharacterized glycosyltransferase genes, alhH and alhK, were knocked out. The resulting mutants, ΔalhH and ΔalhK, showed a notable decrease in EPS yield, reduced molecular weight, and altered monosaccharide compositions. These findings contribute to a better understanding of the alhagan biosynthesis mechanism in P. alhagi NX-11.
Wheat Bran and Saccharomyces Cerevisiae Biomass’ Effect on Aerobic and Anaerobic Degradation Efficiency of Paper Composite
Authors: Markevičiūtė Zita , Guerreschi Arianna , Menin Glauco , Malpei Francesca , Varžinskas Visvaldas
Published: October 2024

This study is a continuation of research on sustainable food packaging materials made from locally available feedstock and industrial by-products within the Baltic Sea region. Its main focus is the impact of wheat bran filler and Saccharomyces cerevisiae additive, which was used to develop a novel bio-coating for paper composite packaging, on the biodegradation efficiency of paper composites under aerobic and anaerobic conditions. In this study, we analyzed the effect of 15% and 40% concentrations of wheat bran filler and Saccharomyces cerevisiae biomass on the biodegradation efficiency of paper composites. This research was conducted under controlled environmental conditions, with aerobic biodegradation tested at 46 °C in a compost-based mesophilic–thermophilic environment and anaerobic biodegradation tested at 55 °C in an active inoculum thermophilic environment. The results show that the presence of wheat bran filler significantly improves biodegradation efficiency compared to microcrystalline cellulose reference material. Under aerobic conditions, the biodegradation efficiency for the 40% wheat bran and yeast sample was 6.34%, compared to only 0.71% for the cellulose reference material. In anaerobic conditions, the 15% wheat bran and yeast sample showed a biodegradation efficiency of 96.62%, compared to 82.32% for the cellulose reference material.
The Composition and Function of Intestinal Microbiota Were Altered in Farmed Bullfrog Tadpoles (Aquarana catesbeiana) during Metamorphosis
Authors: Zheng Xiaoting , Chen Qiuyu , Liang Xueying , Kumar Vikash , Loor Alfredo , Dong Hongbiao , Liu Chang , Yang Jinlong , Zhang Jiasong
Published: October 2024

The bullfrog Aquarana catesbeiana is one of the main farmed frog species in China, with a low overall survival of farmed bullfrogs from hatching to harvest since bullfrog tadpoles are fragile during the metamorphosis period. The intestinal bacterial community can play crucial roles in animal development; however, little is known about the alteration of the gut microbial community of A. catesbeiana during metamorphosis. The present study used 16S rRNA amplicon sequencing to investigate the intestinal bacterial community in A. catesbeiana at four distinct developmental stages. Moreover, we determined the bullfrog’s body morphological parameters and the intestine histology at different developmental stages. The results showed a reduction in the total length and snout–vent length of A. catesbeiana during metamorphosis. The intestinal microbial composition of A. catesbeiana exhibited variation throughout the process of metamorphosis. The terrestrial stage showed shifts in the bacterial composition compared to the aquatic stages, including a reduction in Bacteroidetes and an increase in Firmicutes. Furthermore, the presence of Prevotella, Bifidobacterium, Leucobacter, Corynebacterium, Bulleidia, Dorea, Robinsoniella, and Clostridium in A. catesbeiana metamorphosis appears to be mainly related to the host’s epithelial cells’ height and total body mass. The results indicated that the intestinal microbial composition changed with the bullfrog–tadpole metamorphosis. The genera of Prevotella, Bifidobacterium, Leucobacter, Corynebacterium, Bulleidia, Dorea, Robinsoniella, and Clostridium might be potential probiotics.
Sex-Dependent Rhizosphere Microbial Dynamics and Function in Idesia polycarpa through Floral and Fruit Development
Authors: Li Zhi , Yuan Qiupeng , Wang Shasha , Zhang Tao , Wang Yanmei , Cai Qifei , Geng Xiaodong , Yang Yi , Miao Chao , Dai Li , Rana Sohel , Liu Zhen
Published: October 2024

Male Idesia polycarpa, which display distinct morphological and physiological traits, exhibit greater adaptability to stressful environments than females. However, the connection between this adaptability and rhizosphere processes remains unclear. Here, we investigate the differences in root bacterial community structures between male and female plants at different developmental stages, identifying bacterial strains associated with plant sex through functional predictions. This study aims to inform the optimal allocation of male and female plants during cultivation and provide a theoretical basis for sex identification and breeding. Samples from seven-year-old male and female plants were collected during the flowering (May) and fruit ripening (October) stages. Rhizosphere nutrient content and bacterial diversity were analyzed using Illumina high-throughput sequencing technology. The results demonstrate that total nitrogen (TN), total carbon (TC), and available potassium (AK) varied between sexes at different times. No significant differences between male and female plants were observed in the Shannon, Simpson, and Chao1 indexes during the flowering period. However, the Chao1 and Shannon indexes were significantly higher at fruit maturity in male rather than female plants. The predominant phyla of rhizosphere bacteria were Pseudomonadota, Acidobacteriota, and Actinomycetes. Interestingly, from flowering to fruit ripening, the dominant phyla in both male and female plants shifted from Actinomycetes to Pseudomonadota. A significant correlation was observed between pH and AK and rhizosphere bacteria (p < 0.05), with metabolism being the main functional difference. This study provides preliminary insights into the functional predictions and analyses of bacteria associated with Idesia polycarpa. The above findings lay the groundwork for further investigation into the sex-specific differences in microbial flora across different developmental stages, elucidating the mechanisms underlying flora changes and offering theoretical support for the high-quality management of Idesia polycarpa.
Role of Humic Substances in the (Bio)Degradation of Synthetic Polymers under Environmental Conditions
Authors: Senko Olga , Maslova Olga , Stepanov Nikolay , Aslanli Aysel , Lyagin Ilya , Efremenko Elena
Published: October 2024

Information on the detection of the presence and potential for degradation of synthetic polymers (SPs) under various environmental conditions is of increasing interest and concern to a wide range of specialists. At this stage, there is a need to understand the relationship between the main participants in the processes of (bio)degradation of SPs in various ecosystems (reservoirs with fresh and sea water, soils, etc.), namely the polymers themselves, the cells of microorganisms (MOs) participating in their degradation, and humic substances (HSs). HSs constitute a macrocomponent of natural non-living organic matter of aquatic and soil ecosystems, formed and transformed in the processes of mineralization of bio-organic substances in environmental conditions. Analysis of the main mechanisms of their influence on each other and the effects produced that accelerate or inhibit polymer degradation can create the basis for scientifically based approaches to the most effective solution to the problem of degradation of SPs, including in the form of microplastics. This review is aimed at comparing various aspects of interactions of SPs, MOs, and HSs in laboratory experiments (in vitro) and environmental investigations (in situ) aimed at the biodegradation of polymers, as well as pollutants (antibiotics and pesticides) that they absorb. Comparative calculations of the degradation velocity of different SPs in different environments are presented. A special place in the analysis is given to the elemental chemical composition of HSs, which are most successfully involved in the biodegradation of SPs. In addition, the role of photo-oxidation and photoaging of polymers under the influence of the ultraviolet spectrum of solar radiation under environmental conditions on the (bio)degradation of SPs in the presence of HSs is discussed.
One-Step Soft Agar Enrichment and Isolation of Human Lung Bacteria Inhibiting the Germination of Aspergillus fumigatus Conidia
Authors: Palmieri Fabio , Diserens Jérémy , Gresse Manon , Magnin Margo , Helle Julina , Salamin Benoît , Bisanti Lorenzo , Bernasconi Eric , Pernot Julie , Shanmuganathan Apiha , Trompette Aurélien , von Garnier Christophe , Junier Thomas , Neuenschwander Samuel , Bindschedler Saskia , Pagni Marco , Koutsokera Angela , Ubags Niki , Junier Pilar
Published: October 2024

Fungi of the genus Aspergillus are widespread in the environment, where they produce large quantities of airborne conidia. Inhalation of Aspergillus spp. conidia in immunocompromised individuals can cause a wide spectrum of diseases, ranging from hypersensitivity responses to lethal invasive infections. Upon deposition in the lung epithelial surface, conidia encounter and interact with complex microbial communities that constitute the lung microbiota. The lung microbiota has been suggested to influence the establishment and growth of Aspergillus spp. in the human airways. However, the mechanisms underlying this interaction have not yet been sufficiently investigated. In this study, we aimed to enrich and isolate bacterial strains capable of inhibiting the germination and growth of A. fumigatus conidia from bronchoalveolar lavage fluid (BALF) samples of lung transplant recipients using a novel enrichment method. This method is based on a soft agar overlay plate assay in which bacteria are directly in contact with conidia, allowing inhibition to be readily observed during enrichment. We isolated a total of five clonal bacterial strains with identical genotypic fingerprints, as shown by random amplified polymorphic DNA PCR (RAPD–PCR). All strains were identified as Pseudomonas aeruginosa (strains b1–b5). The strains were able to inhibit the germination and growth of Aspergillus fumigatus in a soft agar confrontation assay, as well as in a germination multiplate assay. Moreover, when compared with ten P. aeruginosa strains isolated from expectoration through standard methods, no significant differences in inhibitory potential were observed. Additionally, we showed inhibition of A. fumigatus growth on Calu-3 cell culture monolayers. However, the isolated P. aeruginosa strains were shown to cause significant damage to the cell monolayers. Overall, although P. aeruginosa is a known opportunistic lung pathogen and antagonist of A. fumigatus, we validated this novel one-step enrichment approach for the isolation of bacterial strains antagonistic to A. fumigatus from BALF samples as a proof-of-concept. This opens up a new venue for the targeted enrichment of antagonistic bacterial strains against specific fungal pathogens.
Silver and Copper Nanoparticles Hosted by Carboxymethyl Cellulose Reduce the Infective Effects of Enterotoxigenic Escherichia coli:F4 on Porcine Intestinal Enterocyte IPEC-J2
Authors: Tchoumi Neree Armelle , Noori Farzaneh , Azzouz Abdelkrim , Costa Marcio , Fairbrother John Morris , Mateescu Mircea Alexandru , Chorfi Younes
Published: October 2024

Zero-valent copper and silver metals (Ms) nanoparticles (NPs) supported on carboxymethylcellulose (CMC) were synthesized for treating Enterotoxigenic Escherichia coli fimbriae 4 (ETEC:F4), a major cause of diarrhea in post-weaned pigs. The antibacterial properties of Cu0/CMC and Ag0/CMC were assessed on infected porcine intestinal enterocyte IPEC-J2, an in vitro model mimicking the small intestine. The lower average particle size (218 nm) and polydispersity index [PDI]: 0.25) for Ag0/CMC, when compared with those of Cu0/CMC (367 nm and PDI 0.96), were explained by stronger Ag0/CMC interactions. The minimal inhibitory concentration (MIC) and half inhibitory concentration (IC50) of Ag0/CMC were lower in both bacteria and IPEC-J2 cells than those of Cu0/CMC, confirming that silver nanoparticles are more bactericidal than copper counterparts. IPEC-J2, less sensitive in MNP/CMC treatment, was used to further investigate the infective process by ETEC:F4. The IC50 of MNP/CMC increased significantly when infected IPEC-J2 cells and ETEC were co-treated, showing an inhibition of the cytotoxicity effect of ETEC:F4 infection and protection of treated IPEC-J2. Thus, it appears that metal insertion in CMC induces an inhibiting effect on ETEC:F4 growth and that MNP/CMC dispersion governs the enhancement of this effect. These results open promising prospects for metal-loaded biopolymers for preventing and treating swine diarrhea.
Effects of Size and Mechanical Pre-Treatment on Aluminium Recovery from Municipal Solid Waste Incineration Bottom Ash
Authors: Gökelma Mertol , Hatipoğlu Utku , Vallejo-Olivares Alicia , Önen Tüzgel Rabia , Kıvrak Olcay , Bazoğlu Elif , Çizen Zeynep Su , Tranell Gabriella
Published: October 2024

Municipal solid waste (MSW) is incinerated to reduce the volume and recover energy and materials. The generation of MSW has been increasing over the past few decades due to the increase in population and changing consumption habits. Rising environmental and economic concerns have increased the importance of waste treatment and recovery. Currently, MSW may take three alternate or parallel routes: direct recycling, incineration, or landfill, depending on the country and location. MSW incineration has three products in addition to energy: bottom ash, fly ash, and off-gas. After incineration, bottom ash usually still contains many materials to be recovered, such as glass, ceramics, and metals with a degree of oxidation. This study focuses on aluminium recovery from MSW incineration bottom ash from two different countries. The 2–30 mm fraction of aluminium particles was characterized in terms of its size, shape, and oxide thickness, and its effects on aluminium recovery were investigated. In addition, the ability of mechanical pre-treatment to remove oxides prior to melting was studied. The results were compared with the analytical modeling developed in this study. An increasing particle size and surface area resulted in an increase in aluminium recovery. Mechanical pre-treatment increased the yield for smaller particles to a larger extent than larger particles due to the difference in the oxide/metal ratio.
Influence of Steel and Poly Vinyl Alcohol Fibers on the Development of High-Strength Geopolymer Concrete
Authors: Hussain Shaik , Matthews John , Amritphale Sudhir , Edwards Richard , Matthews Elizabeth , Paul Niloy , Kraft John
Published: October 2024

The present study focuses on the mechanical performance of steel and polyvinyl alcohol fibers embedded in the geopolymer matrix. A high-strength geopolymer concrete with fly ash, slag and silica fume as precursors and sodium hydroxide and sodium silicate solutions as activators has been tested for its strength in compression and flexure. The influence of fibers on flowability, long-term shrinkage and sulphuric acid attack on the geopolymer concrete has also been studied. The dosage of fibers was maintained at 1%, 2% and 3% by volume, and fibers of length 13 mm have been used in the study. Results indicate that slag with 3% steel fibers by volume had a predominant influence on the strength development of steel fiber-reinforced geopolymer concrete, yielding a compressive strength of 107 MPa after 28 days. Blast furnace slag resulted in increasing the shrinkage of concrete due to rapid gel formation owing to the presence of calcium ions, although the fibers helped reduce the shrinkage to some extent. The strength of steel fiber geopolymer concrete was superior to PVA fiber geopolymer concrete; however, after an acid attack, the strength of steel fiber geopolymer concrete was reduced more than PVA fiber geopolymer concrete due to the enhanced corrosion resistance of PVA fibers.
Mineralogy and Geochemistry of Listvenite-Hosted Ni–Fe Sulfide Paragenesis—A Case Study from Janjevo and Melenica Listvenite Occurrences (Kosovo)
Authors: Kluza Konrad , Pršek Jaroslav , Mederski Sławomir
Published: October 2024

The main goal of this paper is to determine the order of the paragenetic sequence and phase transitions of the Ni–Fe sulfide association hosted in listvenites. Listvenites are hydrothermally altered mafic and ultramafic rocks that are often associated with active tectonic settings, such as transform faults, suture zones, and regional extensional faults, usually in contact with volcanic or carbonate rocks. Listvenitization is displayed by a carbonation process when the original olivine, pyroxene, and serpentine group minerals are altered to Mg–Fe–Ca carbonates (magnesite, calcite, dolomite, and siderite), talc, quartz, and accessory Cr spinel, fuchsite, and Ni–Fe sulfides. The formed rocks are highly reactive; therefore, very often, younger hydrothermal processes are observed, overprinting the mineralogy and geochemistry of the original listvenitization products, including accessory Ni–Fe sulfide paragenesis. The studied samples of listvenites were collected from two locations in Kosovo (Vardar Zone): Janjevo and Melenica. The Ni–Fe sulfide textures and relationships with the surrounding listvenite-hosted minerals were obtained using reflected and transmitted light microscopy, while their chemical composition was determined using an electron microprobe. They form accessory mono-or polymetallic aggregates that usually do not exceed 100 μm in size disseminated in the studied listvenites. Generally, the paragenetic sequence of Ni–Fe sulfides is divided into three stages. The first pre-listvenite magmatic phase is represented by pentlandite and millerite. The second listvenite stage consists of Ni–Co bearing pyrite I (Ni content up to 11.57 wt.% [0.24 apfu], and Co content up to 6.54 wt.% [0.14 apfu]) and differentiated thiospinels (violarite + siegenite ± polydymite). The last, late listvenite stage is represented by younger gersdorffite−ullmannite and base metal mineralization: pyrite + marcasite + sphalerite + galena ± chalcopyrite ± sulfosalts. The findings obtained should help in the interpretation of many disseminated accessory Ni–Fe–Co mineralizations associated with mafic and ultramafic rocks worldwide.
Static Correction of Passive-Source Seismic Reflection Exploration on Undulating Surfaces
Authors: Zheng Jun , Yan Haofei , Liu Guofeng
Published: October 2024

Elevation static correction is an important step in seismic data processing used to eliminate distortions in events caused by surface fluctuations. There is a well-established processing sequence for active-source seismic data, with static correction applied first, followed by subsequent processing. For passive-source data, static correction can be carried out using two methods. The first method is similar to the static correction of active-source seismic data and involves performing static correction on virtual shot gathers. The second method involves directly applying static correction to ambient noise and then using the corrected ambient noise to retrieve the virtual shot gathers. In this study, we derived and compared these two methods to perform static correction on passive-source data. We compared the above two methods through the forward modeling of passive-source data and field data from the Daqiao Gold Mine. The results showed that applying static correction to ambient noise and virtual shot gathers can correct passive-source data from an undulating surface to a flat surface. The equivalence of static correction on virtual shot gathers, and ambient noise offers more choices for conducting static correction of passive-source seismic reflection exploration on undulating surfaces.
Procedure Design and Reliability Analysis for Prediction of Surface Subsidence of a Metal Mine Induced by Block Caving Method—A Case Study of Pulang Copper Mine in China
Authors: Ling Weijia , Zhu Zhonghua , Feng Xinglong , Wang Liguan , Wang Weixiong , Li Zhengrong , Qiu Jiadong
Published: October 2024

Surface subsidence resulting from block caving mining causes considerable environmental and economic harm in mining areas, highlighting the critical need for accurate predictions of surface subsidence. Given the unique features of the block caving technique and the resemblance between the released ore pillars and the mining processes, this paper developed a lightweight model to forecast surface settlement utilizing the probability integration approach to address the issue of predicting surface settlement in metallic mines. This study focuses on the Pulang Copper Mine, situated in the northeast of Shangri-La County within the Yunnan Province, as a case example. This mine employs the block caving method, which results in substantial surface subsidence. A visual mining simulation program is designed to combine the ore mining plan with the prediction model, manage the ore output of each mining point in batches, treat the ore pillars released in the planning cycle as strip work, and simulate and calculate the surface area above the ore pillars settlement value. The calculated values of surface subsidence induced by ore drawing are then interpreted as the downward displacement of the surface subsidence beneath the strip workings. Furthermore, to verify the reliability of the model, three-dimensional laser point cloud data of the Pulang Copper Mine in recent years were collected, and the differences between the predicted surface and the measured surface were calculated and analyzed.
Joint Inversion of DC Resistivity and Gravity Data with Undulating Terrain Based on Deformed Hexahedral Mesh
Authors: Sun Peng , Li Tonglin , Zhang Rongzhe , Yang Hetian , He Haoyuan , Kang Xinze , Liu Xianghao
Published: October 2024

In the field of mineral resource exploration, accurate imaging of subsurface structures is key to discovering and assessing potential mineral deposits. Traditional single geophysical methods, limited by terrain variations and their own constraints, can lead to divergent solutions and structural inconsistencies, affecting the reliability of exploration outcomes. To address these challenges, this paper presents a joint inversion method for three-dimensional direct current (DC) resistivity and gravity data based on a deformed hexahedral mesh. The article begins by outlining the current state of development of the method under study and proposes a research plan, followed by a detailed explanation of the theoretical basis and algorithmic implementation of the proposed method. Model tests confirm the advantages of the deformed hexahedral mesh in reducing terrain impacts and enhancing model resolution, demonstrating the optimization and complementarity of the resolution between the two methods after joint inversion. Finally, applying this method to actual data from the Huaniu Mountain area shows that joint inversion not only improves the consistency of the ore belt structure but also provides a more precise analysis for the quantitative interpretation of the distribution of underground mineral resources. This confirms the method’s effectiveness and potential in practical geological exploration.