bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Impact of Two Generalist Predators on the Leafhopper Erasmoneura vulnerata Population Density
Authors: Prazaru Stefan Cristian , Zanettin Giulia , Pozzebon Alberto , Tirello Paola , Toffoletto Francesco , Scaccini Davide , Duso Carlo
Published: April 2021

Outbreaks of the Nearctic leafhopper Erasmoneura vulnerata represent a threat to vinegrowers in Southern Europe, in particular in North-eastern Italy. The pest outbreaks are frequent in organic vineyards because insecticides labeled for organic viticulture show limited effectiveness towards leafhoppers. On the other hand, the naturally occurring predators and parasitoids of E. vulnerata in vineyards are often not able to keep leafhopper densities at acceptable levels for vine-growers. In this study, we evaluated the potential of two generalist, commercially available predators, Chrysoperla carnea and Orius majusculus, in suppressing E. vulnerata. Laboratory and semi-field experiments were carried out to evaluate both species’ predation capacity on E. vulnerata nymphs. The experiments were conducted on grapevine leaves inside Petri dishes (laboratory) and on potted and caged grapevines (semi-field); in both experiments, the leaves or potted plants were infested with E. vulnerata nymphs prior to predator releases. Both predator species exhibited a remarkable voracity and significantly reduced leafhopper densities in laboratory and semi-field experiments. Therefore, field studies were carried out over two growing seasons in two vineyards. We released 4 O. majusculus adults and 30 C. carnea larvae per m2 of canopy. Predator releases in vineyards reduced leafhopper densities by about 30% compared to the control plots. Results obtained in this study showed that the two predators have a potential to suppress the pest density, but more research is required to define appropriate predator–prey release ratios and release timing. Studies on intraguild interactions and competition with naturally occurring predators are also suggested.
Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer
Authors: Opławski Marcin , Nowakowski Robert , Średnicka Agata , Ochnik Dominika , Grabarek Beniamin Oskar , Boroń Dariusz
Published: April 2021

Modern diagnostics are based on molecular analysis and have been focused on searching for new molecular markers to use in diagnostics. Included in this has been the search for the correlation between gene expression in tissue samples and liquid biological materials. The aim of this study was to evaluate the differences in the expression profile of messenger RNA (mRNA) and micro-RNA (miRNA) related to the epithelial–mesenchymal transition (EMT) in different grades of endometrial cancer (G1–G3), in order to select the most promising molecular markers. The study material consisted of tissue samples and whole blood collected from 30 patients with endometrial cancer (study group; G1 = 15; G2 = 8; G3 = 7) and 30 without neoplastic changes (control group). The molecular analysis included the use of the microarray technique and RTqPCR. Microarray analysis indicated the following number of mRNA differentiating the endometrial cancer samples from the control (tissue/blood): G1 vs. C = 21/18 mRNAs, G2 vs. C = 19/14 mRNAs, and G3 vs. C = 10/9 mRNAs. The common genes for the tissue and blood samples (Fold Change; FC > 3.0) were G1 vs. C: TGFB1, WNT5A, TGFB2, and NOTCH1; G2 vs. C: BCL2L, SOX9, BAMBI, and SMAD4; G3 vs. C STAT1 and TGFB1. In addition, mRNA TGFB1, NOTCH1, and BCL2L are common for all grades of endometrial cancer. The analysis showed that miR-144, miR-106a, and miR-30d are most strongly associated with EMT, making them potential diagnostic markers.
Effect of Earlier Door-to-CT and Door-to-Bleeding Control in Severe Blunt Trauma: A Retrospective Cohort Study
Authors: Murao Shuhei , Yamakawa Kazuma , Kabata Daijiro , Kinoshita Takahiro , Umemura Yutaka , Shintani Ayumi , Fujimi Satoshi
Published: April 2021

Blunt trauma is a potentially life-threatening injury that requires prompt diagnostic examination and therapeutic intervention. Nevertheless, how impactful a rapid response time is on mortality or functional outcomes has not been well-investigated. This study aimed to evaluate effects of earlier door-to-computed tomography time (D2CT) and door-to-bleeding control time (D2BC) on clinical outcomes in severe blunt trauma. This was a single-center, retrospective cohort study of patients with severe blunt trauma (Injury Severity Score > 16). To assess the effect of earlier D2CT and D2BC on clinical outcomes, we conducted multivariable regression analyses with a consideration for nonlinear associations. Among 671 patients with severe blunt trauma who underwent CT scanning, 163 patients received an emergency bleeding control procedure. The median D2CT and D2BC were 19 min and 57 min, respectively. In a Cox proportional hazard regression model, earlier D2CT was not associated with improved 28-day mortality (p = 0.30), but it was significantly associated with decreased mortality from exsanguination (p = 0.003). Earlier D2BC was significantly associated with improved 28-day mortality (p = 0.026). In conclusion, earlier time to a hemostatic procedure was independently associated with decreased mortality. Meanwhile, time benefits of earlier CT examination were not observed for overall survival but were observed for decreased mortality from exsanguination.
Real-World Outcomes of Ivacaftor Treatment in People with Cystic Fibrosis: A Systematic Review
Authors: Duckers Jamie , Lesher Beth , Thorat Teja , Lucas Eleanor , McGarry Lisa J. , Chandarana Keval , De Iorio Fosca
Published: April 2021

Cystic fibrosis (CF) is a rare, progressive, multi-organ genetic disease. Ivacaftor, a small-molecule CF transmembrane conductance regulator modulator, was the first medication to treat the underlying cause of CF. Since its approval, real-world clinical experience on the use of ivacaftor has been documented in large registries and smaller studies. Here, we systematically review data from real-world observational studies of ivacaftor treatment in people with CF (pwCF). Searches of MEDLINE and Embase identified 368 publications reporting real-world studies that enrolled six or more pwCF treated with ivacaftor published between January 2012 and September 2019. Overall, 75 publications providing data from 57 unique studies met inclusion criteria and were reviewed. Studies reporting within-group change for pwCF treated with ivacaftor consistently showed improvements in lung function, nutritional parameters, and patient-reported respiratory and sino-nasal symptoms. Benefits were evident as early as 1 month following ivacaftor initiation and were sustained over long-term follow-up. Decreases in pulmonary exacerbations, Pseudomonas aeruginosa prevalence, and healthcare resource utilization also were reported for up to 66 months following ivacaftor initiation. In studies comparing ivacaftor treatment to modulator untreated comparator groups, clinical benefits similarly were reported as were decreases in mortality, organ-transplantation, and CF-related complications. The safety profile of ivacaftor observed in these real-world studies was consistent with the well-established safety profile based on clinical trial data. Our systematic review of real-world studies shows ivacaftor treatment in pwCF results in highly consistent and sustained clinical benefit in both pulmonary and non-pulmonary outcomes across various geographies, study designs, patient characteristics, and follow-up durations, confirming and expanding upon evidence from clinical trials.
Clinical Characteristics and Degree of Glycemic and Cardiovascular Risk Factor Control in Patients with Type 1 Diabetes in Catalonia (Spain)
Authors: Gimenez-Perez Gabriel , Franch-Nadal Josep , Ortega Emilio , Mata-Cases Manel , Goday Albert , Real Jordi , Rodriguez Angel , Vlacho Bogdan , Mauricio Dídac
Published: April 2021

Background: This study aims to evaluate the clinical characteristics, complications, degree of glycemic control, and cardiovascular risk factor control in patients with type 1 diabetes in Catalonia (Northwest of Spain). Methods: Cross-sectional study using a database including clinical, laboratory, and treatment data. Patients with an ICD10 diagnosis of type 1 diabetes were included, excluding those treated with glucose-lowering agents other than insulin, or treated only with basal insulin two years after diagnosis. Results: 15,008 patients were analysed. Median IQR age was 42 (31–53) years, diabetes duration 11.8 (6.8–16.0) years, 56.5% men. Median (IQR) HbA1c was 7.9% (7.1–8.8). Microvascular complications were present in 24.4% of patients, 43.6% in those with a diabetes duration >19 years. In presence of known cardiovascular disease 69.3% of patients showed an LDL-C concentration >70 mg/dL, 37% had a systolic blood pressure >135 mmHg and 22.4% were smokers. Conclusions: This study provides a reliable snapshot about the clinical situation of a large population of patients with T1D in Catalonia, which is similar to that of other western areas. The lack of adequate control of cardiovascular risk factors in a significant proportion of patients with cardiovascular disease deserves a more detailed analysis and urges the need for improvement strategies.
Drugs That Induce or Cause Deterioration of Myasthenia Gravis: An Update
Authors: Sheikh Shuja , Alvi Usman , Soliven Betty , Rezania Kourosh
Published: April 2021

Myasthenia gravis (MG) is an autoimmune neuromuscular disorder which is characterized by presence of antibodies against acetylcholine receptors (AChRs) or other proteins of the postsynaptic membrane resulting in damage to postsynaptic membrane, decreased number of AChRs or blocking of the receptors by autoantibodies. A number of drugs such as immune checkpoint inhibitors, penicillamine, tyrosine kinase inhibitors and interferons may induce de novo MG by altering the immune homeostasis mechanisms which prevent emergence of autoimmune diseases such as MG. Other drugs, especially certain antibiotics, antiarrhythmics, anesthetics and neuromuscular blockers, have deleterious effects on neuromuscular transmission, resulting in increased weakness in MG or MG-like symptoms in patients who do not have MG, with the latter usually being under medical circumstances such as kidney failure. This review summarizes the drugs which can cause de novo MG, MG exacerbation or MG-like symptoms in nonmyasthenic patients.
Physical Activity and the Risk of COVID-19 Infection and Mortality: A Nationwide Population-Based Case-Control Study
Authors: Cho Dong-Hyuk , Lee Sun Ju , Jae Sae Young , Kim Woo Joo , Ha Seong Jun , Gwon Jun Gyo , Choi Jimi , Kim Dong Wook , Kim Jang Young
Published: April 2021

Regular physical activity (PA) is known to reduce the risk of serious community-acquired infections. We examined the association of PA with the morbidity and mortality resulting from coronavirus disease (COVID-19) infection in the South Korean population. Patients who tested positive for severe acute respiratory coronavirus 2 and who underwent public health screening between 2014 and 2017 (n = 6288) were included. Age- and sex-matched controls (n = 125,772) were randomly selected from the Korean National Health Insurance Service database. Leisure-time PA was assessed using a self-reported questionnaire. The mean PA levels were lower in the patient than in the control group (558.2 ± 516.3 vs. 580.2 ± 525.7 metabolic equivalent of task (MET)-min/week, p = 0.001). Patients with moderate to vigorous PA (MVPA) were associated with a lower risk of COVID-19 morbidity (odds ratio (OR), 0.90; 95% confidence interval (CI), 0.86–0.95). In addition, a standard deviation (SD) increment in MET/week (525.3 MET-min/week) was associated with a 4% decrease in the risk of COVID-19 morbidity (OR, 0.96; 95% CI, 0.93–0.99). MVPA and an SD increment in MET/week were associated with lower mortality (MVPA: OR, 0.47; 95% CI, 0.26–0.87; per SD increment: OR, 0.65; 95% CI, 0.48–0.88). Higher levels of regular PA were associated with a lower risk of COVID-19 infection and mortality, highlighting the importance of maintaining appropriate levels of PA along with social distancing amid the COVID-19 pandemic.
Ship Roll Prediction Algorithm Based on Bi-LSTM-TPA Combined Model
Authors: Wang Yuchao , Wang Hui , Zou Dexin , Fu Huixuan
Published: April 2021

When ships sail on the sea, the changes of ship motion attitude presents the characteristics of nonlinearity and high randomness. Aiming at the problem of low accuracy of ship roll angle prediction by traditional prediction algorithms and single neural network model, a ship roll angle prediction method based on bidirectional long short-term memory network (Bi-LSTM) and temporal pattern attention mechanism (TPA) combined deep learning model is proposed. Bidirectional long short-term memory network extracts time features from the forward and reverse of the ship roll angle time series, and temporal pattern attention mechanism extracts the time patterns from the deep features of a bidirectional long short-term memory network output state that are beneficial to ship roll angle prediction, ignore other features that contribute less to the prediction. The experimental results of real ship data show that the proposed Bi-LSTM-TPA combined model has a significant reduction in MAPE, MAE, and MSE compared with the LSTM model and the SVM model, which verifies the effectiveness of the proposed algorithm.
Evaluation of Social Cognition Measures for Japanese Patients with Schizophrenia Using an Expert Panel and Modified Delphi Method
Authors: Okano Hiroki , Kubota Ryotaro , Okubo Ryo , Hashimoto Naoki , Ikezawa Satoru , Toyomaki Atsuhito , Miyazaki Akane , Sasaki Yohei , Yamada Yuji , Nemoto Takahiro , Mizuno Masafumi
Published: April 2021

Social cognition is strongly linked to social functioning outcomes, making it a promising treatment target. Because social cognition measures tend to be sensitive to linguistic and cultural differences, existing measures should be evaluated based on their relevance for Japanese populations. We aimed to establish an expert consensus on the use of social cognition measures in Japanese populations to provide grounds for clinical use and future treatment development. We assembled a panel of experts in the fields of schizophrenia, social psychology, social neuroscience, and developmental disorders. The panel engaged in a modified Delphi process to (1) affirm expert consensus on the definition of social cognition and its constituent domains, (2) determine criteria to evaluate measures, and (3) identify measures appropriate for Japanese patients with a view toward future quantitative research. Through two online voting rounds and two online video conferences, the panel agreed upon a definition and four-domain framework for social cognition consistent with recent literature. Evaluation criteria for measures included feasibility and tolerability, reliability, clinical effectiveness, validity, and international comparability. The panel finally identified nine promising measures, including one task originally developed in Japan. In conclusion, we established an expert consensus on key discussion points in social cognition and arrived at an expert-selected set of measures. We hope that this work facilitates the use of these measures in Japanese clinical scenarios. We plan to further examine these measures in a psychometric evaluation study.
Evaluation of In Vitro Neuronal Protection by Postconditioning with Poloxamer 188 Following Simulated Traumatic Brain Injury
Authors: Meyer Luise J. , Riess Matthias L.
Published: April 2021

Traumatic brain injury (TBI) leads to morbidity and mortality worldwide. Reperfusion after ischemia adds detrimental injury to cells. Ischemia/reperfusion (I/R) injures cells in a variety of ways including cell membrane disruption. Hence, methods to improve endogenous membrane resealing capacity are crucial. Poloxamer (P) 188, an amphiphilic triblock copolymer, was found to be effective against I/R and mechanical injury in various experimental settings. The aim of this study was to establish an in vitro mouse neuronal TBI model and, further, to investigate if postconditioning with P188 directly interacts with neurons after compression and simulated I/R injury, when administered at the start of reoxygenation. Cellular function was assessed by cell number/viability, mitochondrial viability, membrane damage by lactated dehydrogenase (LDH) release and FM1-43 incorporation as well as apoptosis-activation by Caspase 3. Five hours hypoxia ± compression with 2 h reoxygenation proved to be a suitable model for TBI. Compared to normoxic cells not exposed to compression, cell number and mitochondrial viability decreased, whereas membrane injury by LDH release/FM1-43 dye incorporation and Caspase 3 activity increased in cells exposed to hypoxic conditions with compression followed by reoxygenation. P188 did not protect neurons from simulated I/R and/or compression injury. Future research is indicated.
A General Framework for Mixed and Incomplete Data Clustering Based on Swarm Intelligence Algorithms
Authors: Villuendas-Rey Yenny , Barroso-Cubas Eley , Camacho-Nieto Oscar , Yáñez-Márquez Cornelio
Published: April 2021

Swarm intelligence has appeared as an active field for solving numerous machine-learning tasks. In this paper, we address the problem of clustering data with missing values, where the patterns are described by mixed (or hybrid) features. We introduce a generic modification to three swarm intelligence algorithms (Artificial Bee Colony, Firefly Algorithm, and Novel Bat Algorithm). We experimentally obtain the adequate values of the parameters for these three modified algorithms, with the purpose of applying them in the clustering task. We also provide an unbiased comparison among several metaheuristics based clustering algorithms, concluding that the clusters obtained by our proposals are highly representative of the “natural structure” of data.
The C-Terminus of Perilipin 3 Shows Distinct Lipid Binding at Phospholipid-Oil-Aqueous Interfaces
Authors: Titus Amber R. , Ridgway Ellyse N. , Douglas Rebecca , Brenes Elena Sánchez , Mann Elizabeth K. , Kooijman Edgar E.
Published: April 2021

Lipid droplets (LDs) are ubiquitously expressed organelles; the only intracellular organelles that contain a lipid monolayer rather than a bilayer. Proteins localize and bind to this monolayer as they do to intracellular lipid bilayers. The mechanism by which cytosolic LD binding proteins recognize, and bind, to this lipid interface remains poorly understood. Amphipathic α-helix bundles form a common motif that is shared between cytosolic LD binding proteins (e.g., perilipins 2, 3, and 5) and apolipoproteins, such as apoE and apoLp-III, found on lipoprotein particles. Here, we use pendant drop tensiometry to expand our previous work on the C-terminal α-helix bundle of perilipin 3 and the full-length protein. We measure the recruitment and insertion of perilipin 3 at mixed lipid monolayers at an aqueous-phospholipid-oil interface. We find that, compared to its C-terminus alone, the full-length perilipin 3 has a higher affinity for both a neat oil/aqueous interface and a phosphatidylcholine (PC) coated oil/aqueous interface. Both the full-length protein and the C-terminus show significantly more insertion into a fully unsaturated PC monolayer, contrary to our previous results at the air-aqueous interface. Additionally, the C-terminus shows a preference for lipid monolayers containing phosphatidylethanolamine (PE), whereas the full-length protein does not. These results strongly support a model whereby both the N-terminal 11-mer repeat region and C-terminal amphipathic α-helix bundle domains of perilipin 3 have distinct lipid binding, and potentially biological roles.
Self-Expanding Anchors for Stabilizing Percutaneously Implanted Microdevices in Biological Tissues
Authors: Bhagavatula Sharath , Thompson Devon , Dominas Christine , Haider Irfanullah , Jonas Oliver
Published: April 2021

Percutaneously implanted miniaturized devices such as fiducial markers, miniaturized sensors, and drug delivery devices have an important and expanding role in diagnosing and treating a variety of diseases. However, there is a need to develop and evaluate anchoring methods to ensure that these microdevices remain secure without dislodgement, as even minimal migration within tissues could result in loss of microdevice functionality or clinical complications. Here we describe two anchoring methods made from biocompatible materials: (1) a self-expanding nitinol mesh anchor and (2) self-expanding hydrogel particles contained within pliable netting. We integrate these anchors into existing drug-screening microdevices and experimentally measure forces required to dislodge them from varying tissues. We report similar dislodgement forces of 738 ± 37, 707 ± 40, 688 ± 29, and 520 ± 28 mN for nitinol-anchored microdevices, and 735 ± 98, 702 ± 46, 457 ± 47, and 459 ± 39 mN for hydrogel-anchored microdevices in liver, kidney, fat, and muscle tissues, respectively—significantly higher compared with 13 ± 2, 15 ± 3, 15 ± 2, and 15 ± 3 mN for non-anchored microdevices (p < 0.001 in all tissues). The anchoring methods increased resistance to dislodgement by a factor of 30–50× in all tissues, did not increase the required needle gauge for insertion, and were compatible with percutaneous implantation and removal. These results indicate that anchoring significantly improves microdevice stability and should reduce migration risk in a variety of biological tissues.
Human Endogenous Retroviruses in Glioblastoma Multiforme
Authors: Yuan Zihao , Yang Yuntao , Zhang Ningyan , Soto Claudio , Jiang Xiaoqian , An Zhiqiang , Zheng Wenjin Jim
Published: April 2021

Glioblastoma multiforme (GBM) is the most aggressive and deadly brain tumor. It is primarily diagnosed in the elderly and has a 5-year survival rate of less than 6% even with the most aggressive therapies. The lack of biomarkers has made the development of immunotherapy for GBM challenging. Human endogenous retroviruses (HERVs) are a group of viruses with long terminal repeat (LTR) elements, which are believed to be relics from ancient viral infections. Recent studies have found that those repetitive elements play important roles in regulating various biological processes. The differentially expressed LTR elements from HERVs are potential biomarkers for immunotherapy to treat GBM. However, the understanding of the LTR element expression in GBM is greatly lacking. Methods: We obtained 1077.4 GB of sequencing data from public databases. These data were generated from 111 GBM tissue studies, 30 GBM cell lines studies, and 45 normal brain tissues studies. We analyzed repetitive elements that were differentially expressed in GBM and normal brain samples. Results: We found that 48 LTR elements were differentially expressed (p-value < 0.05) between GBM and normal brain tissues, of which 46 were HERV elements. Among these 46 elements, 34 significantly changed HERVs belong to the ERV1 superfamily. Furthermore, 43 out of the 46 differentially expressed HERV elements were upregulated. Conclusion: Our results indicate significant differential expression of many HERV LTR elements in GBM and normal brain tissues. Expression levels of these elements could be developed as biomarkers for GBM treatments.
Phylogenomic Investigation of Increasing Fluoroquinolone Resistance among Belgian Cases of Shigellosis between 2013 and 2018 Indicates Both Travel-Related Imports and Domestic Circulation
Authors: Bogaerts Bert , Winand Raf , Van Braekel Julien , Mattheus Wesley , De Keersmaecker Sigrid C. J. , Roosens Nancy H. C. , Marchal Kathleen , Vanneste Kevin , Ceyssens Pieter-Jan
Published: April 2021

Shigellosis is an acute enteric infection caused mainly by the species Shigella flexneri and Shigella sonnei. Since surveillance of these pathogens indicated an increase in ciprofloxacin-resistant samples collected in Belgium between 2013 and 2018, a subset of 148 samples was analyzed with whole genome sequencing (WGS) to investigate their dispersion and underlying genomic features associated with ciprofloxacin resistance. A comparison between observed phenotypes and WGS-based resistance prediction to ciprofloxacin revealed perfect correspondence for all samples. Core genome multi-locus sequence typing and single nucleotide polymorphism-typing were used for phylogenomic investigation to characterize the spread of these infections within Belgium, supplemented with data from international reference collections to place the Belgian isolates within their global context. For S. flexneri, substantial diversity was observed with ciprofloxacin-resistant isolates assigned to several phylogenetic groups. Besides travel-related imports, several clusters of highly similar Belgian isolates could not be linked directly to international travel suggesting the presence of domestically circulating strains. For S. sonnei, Belgian isolates were all limited to lineage III, and could often be traced back to travel to countries in Asia and Africa, sometimes followed by domestic circulation. For both species, several clusters of isolates obtained exclusively from male patients were observed. Additionally, we illustrated the limitations of conventional serotyping of S. flexneri, which was impacted by serotype switching. This study contributes to a better understanding of the spread of shigellosis within Belgium and internationally, and highlights the added value of WGS for the surveillance of this pathogen.
Electrospun Shape Memory Polymer Micro-/Nanofibers and Tailoring Their Roles for Biomedical Applications
Authors: Zare Mohadeseh , Davoodi Pooya , Ramakrishna Seeram
Published: April 2021

Shape memory polymers (SMPs) as a relatively new class of smart materials have gained increasing attention in academic research and industrial developments (e.g., biomedical engineering, aerospace, robotics, automotive industries, and smart textiles). SMPs can switch their shape, stiffness, size, and structure upon being exposed to external stimuli. Electrospinning technique can endow SMPs with micro-/nanocharacteristics for enhanced performance in biomedical applications. Dynamically changing micro-/nanofibrous structures have been widely investigated to emulate the dynamical features of the ECM and regulate cell behaviors. Structures such as core-shell fibers, developed by coaxial electrospinning, have also gained potential applications as drug carriers and artificial blood vessels. The clinical applications of micro-/nanostructured SMP fibers include tissue regeneration, regulating cell behavior, cell growth templates, and wound healing. This review presents the molecular architecture of SMPs, the recent developments in electrospinning techniques for the fabrication of SMP micro-/nanofibers, the biomedical applications of SMPs as well as future perspectives for providing dynamic biomaterials structures.
Carbon Dot Nanoparticles: Exploring the Potential Use for Gene Delivery in Ophthalmic Diseases
Authors: Biswal Manas R. , Bhatia Sofia
Published: April 2021

Ocular gene therapy offers significant potential for preventing retinal dystrophy in patients with inherited retinal dystrophies (IRD). Adeno-associated virus (AAV) based gene transfer is the most common and successful gene delivery approach to the eye. These days, many studies are using non-viral nanoparticles (NPs) as an alternative therapeutic option because of their unique properties and biocompatibility. Here, we discuss the potential of carbon dots (CDs), a new type of nanocarrier for gene delivery to the retinal cells. The unique physicochemical properties of CDs (such as optical, electronic, and catalytic) make them suitable for biosensing, imaging, drug, and gene delivery applications. Efficient gene delivery to the retinal cells using CDs depends on various factors, such as photoluminescence, quantum yield, biocompatibility, size, and shape. In this review, we focused on different approaches used to synthesize CDs, classify CDs, various pathways for the intake of gene-loaded carbon nanoparticles inside the cell, and multiple studies that worked on transferring nucleic acid in the eye using CDs.
Measurements of Bicarbonate in Water Containing Ocean-Level Sulfate Using a Simple Multi-Pass Optical Raman System
Authors: Shende Chetan , Farquharson Stuart , Farquharson Duncan , Brouillette Carl
Published: April 2021

The concentration of dissolved inorganic carbon in the oceans at depths of a few meters to thousands of meters is a critical parameter for understanding global warming. The concentration is both pH dependent and depth dependent. Current analysis that employs pH meters must account for several other parameters, such as salinity, temperature, pressure, and the dissolved carbon’s form, carbon dioxide, bicarbonate, or carbonate. Recently, Raman spectroscopy has been used to measure these forms directly in water at ~1000 ppm, which is unfortunately insufficient for typical ocean concentrations, such as ~115 ppm bicarbonate near the surface. Here, we employed a simple multi-pass optical system, a flat mirror to reflect the laser back through the sample, and a concave mirror opposite the entrance slit that effectively doubled the laser power and the collected Raman photons, respectively. This multi-pass optical Raman system with a 1.5 W, 532 nm laser was used to measure 30 ppm bicarbonate in water that contained 2650 ppm sulfate to simulate ocean water, a bicarbonate concentration well below that near the ocean surface. Furthermore, spectral analysis employed the bicarbonate C=O symmetric stretch at 1360 cm−1 instead of the C–OH stretch at 1015 cm−1 to avoid the intense, overlapping sulfate SO4 symmetric stretch at 985 cm−1. The calculated standard deviation of ~5 ppm for the described approach suggests that accurate measurement of bicarbonate in situ is possible, which has been, heretofore, either calculated based on pH or measured in a lab.
Influence of Soil-Borne Inoculum of Plasmodiophora brassicae Measured by qPCR on Disease Severity of Clubroot-Resistant Cultivars of Winter Oilseed Rape (Brassica napus L.)
Authors: Wallenhammar Ann-Charlotte , Omer Zahra Saad , Edin Eva , Jonsson Anders
Published: April 2021

Use of resistant cultivars is considered the most effective tool in managing clubroot. Three clubroot-resistant commercial winter oilseed rape (OSR) cultivars and a susceptible ‘Cultivar mix’ were evaluated for disease severity index (DSI) and yield performance in field soils, selected for varying abundance of natural inoculum of Plasmodiophora brassicae. Seven field trials were carried out during 2017–2019 in winter OSR crops, and comparative bioassays were performed in a growth chamber. Substantial variation in clubroot infection between years was observed in the field trials. For Cultivar mix, a negative correlation (y = −252.3ln(x) + 58,897.6) was found between inoculum density and seed yield in five trials, whereas no correlation was found for the resistant cultivars. In bioassays, Cultivar mix exhibited a significantly high correlation between DSIb and number of gene copies g−1 soil (R2 = 0.72). For resistant cvs., Mentor and Alister, correlation was R2 = 0.45 and 0.58, respectively, indicating that resistance was under pressure. In field trials, DSIf of the resistant cultivars was lower (<27). The recommendation is thus to use clubroot-resistant cultivars of OSR as part of Integrated Pest Management in situations where abundance of P. brassicae DNA exceeds 1300 gene copies g−1 soil.
Genetic Risk Factors of Creutzfeldt-Jakob Disease in the Population of Newborns in Slovakia
Authors: Kosorinova Dana , Belay Girma , Zakova Dana , Stelzer Martin , Mitrova Eva
Published: April 2021

The most frequent human prion disease is Creutzfeldt–Jakob disease (CJD). It occurs as sporadic (sCJD), genetic (gCJD), iatrogenic (iCJD) form and as variant CJD. The genetic form represents about 10–15% of confirmed cases worldwide, in Slovakia as much as 65–75%. Focal accumulation of gCJD was confirmed in Orava region. The most common point mutation of the prion protein gene (PRNP) is E200K. CJD has a long asymptomatic phase and it is not known when the carriers of the mutation E200K become infectious. Precautions to prevent iCJD are focused especially on clinical CJD cases, but asymptomatic CJD-specific mutation carriers cannot be excluded, and represent a potential genetic CJD-risk group. The aim of this study was to determine the occurrence, frequency and geographic distribution of the E200K mutation among the newborns, comparing the areas of focal accumulation of gCJD with extra-focal ones, as well as distribution of the polymorphism M129V of the PRNP gene. A total of 2915 samples of dry blood spots from anonymous newborns were analyzed. We used RealTime PCR method to determine the presence of the E200K mutation and the M129V polymorphism. Genetic testing revealed 13 carriers of the E200K mutation. Investigation of the M129V polymorphism affirmed higher representation of methionine homozygotes (48% MM, 44% MV, 8% VV). Achieved results fully confirmed our previous observations concerning both the specific and nonspecific genetic CJD risk among the Slovak general population. The 48% of methionine homozygotes and 4 carriers of the E200K mutation among 1000 live-born children in Slovakia underline the benefits of genetic testing.
Factors Determining the Implementation of Measures Aimed at Preventing Zoonotic Diseases in Veterinary Practices
Authors: Renault Véronique , Fontaine Sébastien , Saegerman Claude
Published: April 2021

Background: Zoonoses prevention relies mainly on the implementation of different biosecurity measures. This study aimed to assess the level of implementation of biosecurity measures by veterinary practitioners and students and to identify the possible behaviour change determinants. Methods: The data was collected through a cross-sectional survey (N = 382). Statistical analyses were implemented based on the Health Belief Model to identify the possible determinant of the behaviours and the explanatory variables of the perceptions. Results: The survey showed a good level of implementation of the biosecurity measures (median of 81%). The implementation was associated with a higher perception of the zoonoses’ susceptibility and the measures’ benefits, and with a lower perception of the zoonoses’ severity. The study also revealed that the decision to implement a measure was mainly taken on a case-by-case basis depending on the perceived risk of exposure related to a specific context or intervention. Conclusion: The main determining factors identified for the implementation of biosecurity measures (BSMs) were the risk susceptibility and the benefits of the biosecurity measures, which could be influenced by evidence-based communication. The methodology developed can be applied regularly and in other countries to better capture these changes in perceptions over time.
Heating Device Based on Modified Microwave Oven: Improved to Measure Liquid Temperature by Using FBG Sensors
Authors: Garavito Jesus , Galvis Carlos , López Ana Milena , Franco Arlet Patricia , Barreiro Francisco , Tarazona Rosa Liliana , Serpa-Imbett Claudia Milena
Published: April 2021

A prototype device based on a modified domestic microwave oven for liquid food products pasteurization is presented. This novel design has a coiling glass pipe adapted inside a microwave cavity to allow liquid continuous flow, in which several temperature optical sensors based on Fiber Bragg Grating were installed to measure, by means of contact, the circulating liquid temperature in the pipe, while the oven is on, to obtain the profile temperature as a function of time at different pipe points. The temperatures at liquid input and output were also measured with thermocouples. This device was tested to establish how well it may perform using different standardized liquids with well-known physicochemical and dielectric properties, such as water, water with dissolved sugars, water with dissolved salts, and water with dissolved sugars and salts. It could be observed that the maximum temperature reached was 90 °C for distilled water, 80 °C for water with dissolved salts, 60 °C for water with dissolved sugars and 80 °C for water with dissolved sugars and salts, showing that these data were in agreement with previous results in the literature. This type of device would be potentially useful to establish the device’s efficiency in terms of retention time, energy consumption, and volume of processed liquid, thus, broadening the use of this microwave heating technology with several types of liquid substances.
Genome-Wide Mapping of Histone H3 Lysine 4 Trimethylation (H3K4me3) and Its Involvement in Fatty Acid Biosynthesis in Sunflower Developing Seeds
Authors: Moreno-Pérez Antonio J. , Santos-Pereira José M. , Martins-Noguerol Raquel , DeAndrés-Gil Cristina , Troncoso-Ponce M. Adrián , Venegas-Calerón Mónica , Sánchez Rosario , Garcés Rafael , Salas Joaquín J. , Tena Juan J. , Martínez-Force Enrique
Published: April 2021

Histone modifications are of paramount importance during plant development. Investigating chromatin remodeling in developing oilseeds sheds light on the molecular mechanisms controlling fatty acid metabolism and facilitates the identification of new functional regions in oil crop genomes. The present study characterizes the epigenetic modifications H3K4me3 in relationship with the expression of fatty acid-related genes and transcription factors in developing sunflower seeds. Two master transcriptional regulators identified in this analysis, VIV1 (homologous to Arabidopsis ABI3) and FUS3, cooperate in the regulation of WRINKLED 1, a transcriptional factor regulating glycolysis, and fatty acid synthesis in developing oilseeds.
Polytetrafluoroethylene Films in Rigid Polyurethane Foams’ Dielectric Permittivity Measurements with a One-Side Access Capacitive Sensor
Authors: Beverte Ilze , Cabulis Ugis , Gaidukovs Sergejs
Published: April 2021

As a non-metallic composite material, widely applied in industry, rigid polyurethane (PUR) foams require knowledge of their dielectric properties. In experimental determination of PUR foams’ dielectric properties protection of one-side capacitive sensor’s active area from adverse effects caused by the PUR foams’ test objects has to be ensured. In the given study, the impact of polytetrafluoroethylene (PTFE) films, thickness 0.20 mm and 0.04 mm, in covering or simulated coating the active area of one-side access capacitive sensor’ electrodes on the experimentally determined true dielectric permittivity spectra of rigid PUR foams is estimated. Penetration depth of the low frequency excitation field into PTFE and PUR foams is determined experimentally. Experiments are made in order to evaluate the difference between measurements on single PUR foams’ samples and on complex samples “PUR foams + PTFE film” with two calibration modes. A modification factor and a small modification criterion are defined and values of modifications are estimated in numerical calculations. Conclusions about possible practical applications of PTFE films in dielectric permittivity measurements of rigid PUR foams with one-side access capacitive sensor are made.
Comparison of a Novel Polymeric Hollow Fiber Heat Exchanger and a Commercially Available Metal Automotive Radiator
Authors: Kroulíková Tereza , Kůdelová Tereza , Bartuli Erik , Vančura Jan , Astrouski Ilya
Published: April 2021

A novel heat exchanger for automotive applications developed by the Heat Transfer and Fluid Flow Laboratory at the Brno University of Technology, Czech Republic, is compared with a conventional commercially available metal radiator. The heat transfer surface of this heat exchanger is composed of polymeric hollow fibers made from polyamide 612 by DuPont (Zytel LC6159). The cross-section of the polymeric radiator is identical to the aluminum radiator (louvered fins on flat tubes) in a Skoda Octavia and measures 720 × 480 mm. The goal of the study is to compare the functionality and performance parameters of both radiators based on the results of tests in a calibrated air wind tunnel. During testing, both heat exchangers were tested in conventional conditions used for car radiators with different air flow and coolant (50% ethylene glycol) rates. The polymeric hollow fiber heat exchanger demonstrated about 20% higher thermal performance for the same air flow. The efficiency of the polymeric radiator was in the range 80–93% and the efficiency of the aluminum radiator was in the range 64–84%. The polymeric radiator is 30% lighter than its conventional metal competitor. Both tested radiators had very similar pressure loss on the liquid side, but the polymeric radiator featured higher air pressure loss.
Corrosion Resistance of Cr–Co Alloys Subjected to Porcelain Firing Heat Treatment—In Vitro Study
Authors: Rylska Dorota , Januszewicz Bartłomiej , Sokołowski Grzegorz , Sokołowski Jerzy
Published: April 2021

The procedure of ceramics fusion to cobalt–chromium (Co–Cr) base dental crowns affects their corrosion behavior and biological tolerance. This study’s purpose was to comparatively evaluate the effect of heat treatment (HT) applicable for dental ceramics firing on the corrosion properties among Co–Cr base alloys fabricated via different methods: casting (CST), milling soft metal and post sintering (MSM), and selective laser melting (SLM). All specimens were subjected to a heat treatment corresponding to a full firing schedule. The microstructure and elemental composition of oxidized surfaces were investigated by scanning electron microscopy and energy dispersive spectroscopy. Corrosion properties were examined by electrochemical potentiodynamic polarization tests. The values of jcorr, Ecorr, Rp, and breakdown potential Ebr were estimated. The oxide layers formed during the HT process corresponded to the composition of the original alloys’ structure. Among the thermal treated alloys, SLM showed the highest corrosion resistance, followed by the MSM and CST. This may be attributed to uniform distribution of alloying elements in homogenous structure and to the reduced porosity, which enhances corrosion resistance and decreases the risk of crevice corrosion. The overall corrosion behavior was strongly influenced by the segregation of alloying elements in the microstructure, thus, is directly determined by the manufacturing method.
Role of Sparger Configuration in Determining Flotation Performance under Oscillatory Air Supply
Authors: Ji Yiming , Song Ajuan , Li Chao , Cao Yijun
Published: April 2021

Bubble size is crucial for determining flotation efficiency. Fine bubbles can be cost-effectively generated using a multi-orifice sparger with oscillatory air supply. Sparger configuration is defined by the orifice size, the plate thickness and the chamber volume. To date, the effect of sparger configuration on bubble size with oscillatory air supply is not clear yet. To facilitate the control of bubble size formed with oscillatory air supply, the present work investigated the dependence of bubble size on sparger configuration. It was found that bubble size was positively correlated with the chamber volume and the orifice size, while a nonlinear relationship was observed with the plate thickness. Besides, it was found that flotation recovery decreased over increasing bubble size when changing the sparger configuration. The results indicated that sparger configuration exhibited a significant effect on flotation performance via influencing bubble size when oscillatory air supply was applied.
The Protein Interactome of Glycolysis in Escherichia coli
Authors: Chowdhury Shomeek , Hepper Stephen , Lodi Mudassir K. , Saier Milton H. , Uetz Peter
Published: April 2021

Glycolysis is regulated by numerous mechanisms including allosteric regulation, post-translational modification or protein-protein interactions (PPI). While glycolytic enzymes have been found to interact with hundreds of proteins, the impact of only some of these PPIs on glycolysis is well understood. Here we investigate which of these interactions may affect glycolysis in E. coli and possibly across numerous other bacteria, based on the stoichiometry of interacting protein pairs (from proteomic studies) and their conservation across bacteria. We present a list of 339 protein-protein interactions involving glycolytic enzymes but predict that ~70% of glycolytic interactors are not present in adequate amounts to have a significant impact on glycolysis. Finally, we identify a conserved but uncharacterized subset of interactions that are likely to affect glycolysis and deserve further study.
The Effect of Article Characteristics on Citation Number in a Diachronic Dataset of the Biomedical Literature on Chronic Inflammation: An Analysis by Ensemble Machines
Authors: Galli Carlo , Guizzardi Stefano
Published: April 2021

Citations are core metrics to gauge the relevance of scientific literature. Identifying features that can predict a high citation count is therefore of primary importance. For the present study, we generated a dataset of 121,640 publications on chronic inflammation from the Scopus database, containing data such as titles, authors, journal, publication date, type of document, type of access and citation count, ranging from 1951 to 2021. Hence we further computed title length, author count, title sentiment score, number of colons, semicolons and question marks in the title and we used these data as predictors in Gradient boosting, Bagging and Random Forest regressors and classifiers. Based on these data, we were able to train these machines, and Gradient Boosting achieved an F1 score of 0.552 on classification. These models agreed that document type, access type and number of authors were the best predicting factors, followed by title length.
The Importance of Betting Early
Authors: Innocenti Alessandro , Nannicini Tommaso , Ricciuti Roberto
Published: April 2021

We evaluate the impact of timing on decision outcomes when both the timing and the relevant decision are chosen under uncertainty. Sports betting provides the testing ground, as we exploit an original dataset containing more than one million online bets on games of the Italian Major Soccer League. We find that individuals perform systematically better when they place their bets farther away from the game day. The better performance of early bettors holds controlling for (time-invariant) unobservable ability, learning during the season, and timing of the odds. We attribute this result to the increase of noisy information on game day, which hampers the capacity of late (non-professional) bettors to use very simple prediction methods, such as team rankings or last game results. We also find that more successful bettors tend to bet in advance, focus on a smaller set of events, and prefer games associated with smaller betting odds.
Satellite-Based Observations Reveal the Altitude-Dependent Patterns of SIFyield and Its Sensitivity to Ambient Temperature in Tibetan Meadows
Authors: Chen Ruonan , Liu Liangyun , Liu Xinjie
Published: April 2021

Photosynthesis and its sensitivity to the changing environment in alpine regions are of great significance to the understanding of vegetation–environment interactions and other global ecological processes in the context of global change, while their variations along the elevation gradient remain unclear. Using solar-induced chlorophyll fluorescence (SIF) derived from satellite observations, we discovered an increase in solar-induced fluorescence yield (SIFyield) with rising elevation in Tibetan meadows in the summer, related to the altitudinal variation in temperature sensitivity at both seasonal and interannual scales. Results of the altitudinal patterns of SIFyield demonstrated higher temperature sensitivity at high altitudes, and the sensitivity at the interannual scale even exceeds that at seasonal scale when the elevation reaches above 4700 m. This high-temperature sensitivity of SIFyield at high altitudes implies potential adaptation of alpine plants and also indicates that changes in photosynthesis-related physiological functions at high altitudes should receive more attention in climate change research. The altitudinal SIFyield patterns revealed in this study also highlight that variations in temperature sensitivity should be considered in models, otherwise the increasing trend of SIFyield observations can never be discovered in empirical simulations.