bluuebunny/crossref_metadata_2025 huggingface dataset:
Insight into the Molecular Interaction of Cloxyquin (5-chloro-8-hydroxyquinoline) with Bovine Serum Albumin: Biophysical Analysis and Computational Simulation
Authors: Phopin Kamonrat , Ruankham Waralee , Prachayasittikul Supaluk , Prachayasittikul Virapong , Tantimongcolwat Tanawut
Published: January 2020

Cloxyquin is a potential therapeutic compound possessing various bioactivities, especially antibacterial, antifungal, cardioprotective, and pain relief activities. Herein, the interaction mechanism between cloxyquin and bovine serum albumin (BSA) has been elucidated in order to fulfill its pharmacokinetic and pharmacodynamic gaps essential for further development as a therapeutic drug. Multi-spectroscopic and biophysical model analysis suggested that cloxyquin interacts with BSA via a static process by ground-state complex formation. Its binding behavior emerged as a biphasic fashion with a moderate binding constant at the level of 104 M−1. Thermodynamic analysis and molecular docking simulation concurrently revealed that hydrophobic interaction is a major driving force for BSA–cloxyquin complexation. Binding of cloxyquin tends to slightly enlarge the monomeric size of BSA without a significant increase of aggregate fraction. Cloxyquin preferentially binds into the fatty acid binding site 5 (FA5) of the BSA via hydrophobic interaction amongst its quinoline scaffold and Phe550, Leu531, and Leu574 residues of BSA. The quinoline ring and hydroxyl moiety of cloxyquin also form the π–π interaction and the hydrogen bond with Phe506. Our data indicate a potential function of serum albumin as a carrier of cloxyquin in blood circulation.
Role of Metastasis-Related Genes in Cisplatin Chemoresistance in Gastric Cancer
Authors: Nishiguchi Yukiko , Oue Naohide , Fujiwara-Tani Rina , Sasaki Takamitsu , Ohmori Hitoshi , Kishi Shingo , Mori Shiori , Mori Takuya , Ikeda Naoya , Matsumoto Sohei , Wakatsuki Kohei , Luo Yi , Yasui Wataru , Sho Masayuki , Kuniyasu Hiroki
Published: January 2020

The role of metastasis-related genes in cisplatin (CDDP) chemoresistance in gastric cancer is poorly understood. Here, we examined the expression of four metastasis-related genes (namely, c-met, HMGB1, RegIV, PCDHB9) in 39 cases of gastric cancer treated with neoadjuvant therapy with CDDP or CDDP+5-fluorouracil and evaluated its association with CDDP responsiveness. Comparison of CDDP-sensitive cases with CDDP-resistant cases, the expression of c-met, HMGB1, and PCDHB9 was correlated with CDDP resistance. Among them, the expression of HMGB1 showed the most significant correlation with CDDP resistance in multivariate analysis. Treatment of TMK-1 and MKN74 human gastric cancer cell lines with ethyl pyruvate (EP) or tanshinone IIA (TAN), which are reported to inhibit HMGB1 signaling, showed a 4–5-fold increase in inhibition by CDDP. Treatment with EP or TAN also suppressed the expression of TLR4 and MyD88 in the HMGB1 signal transduction pathway and suppressed the activity of NFκB in both cell lines. These results suggest that the expression of these cancer metastasis-related genes is also related to anticancer drug resistance and that suppression of HMGB1 may be particularly useful for CDDP sensitization.
The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner
Authors: Hoang Xuan , Nguyen Nguyen , Nguyen Yen-Nhi , Watanabe Yasuko , Tran Lam-Son , Thao Nguyen
Published: January 2020

Being master regulators of gene expression, transcription factors (TFs) play important roles in determining plant growth, development and reproduction. To date, many TFs have been shown to positively mediate plant responses to environmental stresses. In the current study, the biological functions of a stress-responsive NAC [NAM (No Apical Meristem), ATAF1/2 (Arabidopsis Transcription Activation Factor1/2), CUC2 (Cup-shaped Cotyledon2)]-TF encoding gene isolated from soybean (GmNAC019) in relation to plant drought tolerance and abscisic acid (ABA) responses were investigated. By using a heterologous transgenic system, we revealed that transgenic Arabidopsis plants constitutively expressing the GmNAC019 gene exhibited higher survival rates in a soil-drying assay, which was associated with lower water loss rate in detached leaves, lower cellular hydrogen peroxide content and stronger antioxidant defense under water-stressed conditions. Additionally, the exogenous treatment of transgenic plants with ABA showed their hypersensitivity to this phytohormone, exhibiting lower rates of seed germination and green cotyledons. Taken together, these findings demonstrated that GmNAC019 functions as a positive regulator of ABA-mediated plant response to drought, and thus, it has potential utility for improving plant tolerance through molecular biotechnology.
L-DOPA in Parkinson’s Disease: Looking at the “False” Neurotransmitters and Their Meaning
Authors: Chagraoui Abdeslam , Boulain Marie , Juvin Laurent , Anouar Youssef , Barrière Grégory , Deurwaerdère Philippe De
Published: January 2020

L-3,4-dihydroxyphenylalanine (L-DOPA) has been successfully used in the treatment of Parkinson’s disease (PD) for more than 50 years. It fulfilled the criteria to cross the blood–brain barrier and counteract the biochemical defect of dopamine (DA). It remarkably worked after some adjustments in line with the initial hypothesis, leaving a poor place to the plethora of mechanisms involving other neurotransmitters or mechanisms of action beyond newly synthesized DA itself. Yet, its mechanism of action is far from clear. It involves numerous distinct cell populations and does not mimic the mechanism of action of dopaminergic agonists. L-DOPA-derived DA is mainly released by serotonergic neurons as a false neurotransmitter, and serotonergic neurons are involved in L-DOPA-induced dyskinesia. The brain pattern and magnitude of DA extracellular levels together with this status of false neurotransmitters suggest that the striatal effects of DA via this mechanism would be minimal. Other metabolic products coming from newly formed DA or through the metabolism of L-DOPA itself could be involved. These compounds can be trace amines and derivatives. They could accumulate within the terminals of the remaining monoaminergic neurons. These “false neurotransmitters,” also known for some of them as inducing an “amphetamine-like” mechanism, could reduce the content of biogenic amines in terminals of monoaminergic neurons, thereby impairing the exocytotic process of monoamines including L-DOPA-induced DA extracellular outflow. The aim of this review is to present the mechanism of action of L-DOPA with a specific attention to “false neurotransmission.”
Characterization of a Maltase from an Early-Diverged Non-Conventional Yeast Blastobotrys adeninivorans
Authors: Visnapuu Triinu , Meldre Aivar , Põšnograjeva Kristina , Viigand Katrin , Ernits Karin , Alamäe Tiina
Published: January 2020

Genome of an early-diverged yeast Blastobotrys (Arxula) adeninivorans (Ba) encodes 88 glycoside hydrolases (GHs) including two α-glucosidases of GH13 family. One of those, the rna_ARAD1D20130g-encoded protein (BaAG2; 581 aa) was overexpressed in Escherichia coli, purified and characterized. We showed that maltose, other maltose-like substrates (maltulose, turanose, maltotriose, melezitose, malto-oligosaccharides of DP 4‒7) and sucrose were hydrolyzed by BaAG2, whereas isomaltose and isomaltose-like substrates (palatinose, α-methylglucoside) were not, confirming that BaAG2 is a maltase. BaAG2 was competitively inhibited by a diabetes drug acarbose (Ki = 0.8 µM) and Tris (Ki = 70.5 µM). BaAG2 was competitively inhibited also by isomaltose-like sugars and a hydrolysis product—glucose. At high maltose concentrations, BaAG2 exhibited transglycosylating ability producing potentially prebiotic di- and trisaccharides. Atypically for yeast maltases, a low but clearly recordable exo-hydrolytic activity on amylose, amylopectin and glycogen was detected. Saccharomyces cerevisiae maltase MAL62, studied for comparison, had only minimal ability to hydrolyze these polymers, and its transglycosylating activity was about three times lower compared to BaAG2. Sequence identity of BaAG2 with other maltases was only moderate being the highest (51%) with the maltase MalT of Aspergillus oryzae.
Targeting the 16S rRNA Gene for Bacterial Identification in Complex Mixed Samples: Comparative Evaluation of Second (Illumina) and Third (Oxford Nanopore Technologies) Generation Sequencing Technologies
Authors: Winand Raf , Bogaerts Bert , Hoffman Stefan , Lefevre Loïc , Delvoye Maud , Van Braekel Julien , Fu Qiang , Roosens Nancy HC , De Keersmaecker Sigrid CJ , Vanneste Kevin
Published: January 2020

Rapid, accurate bacterial identification in biological samples is an important task for microbiology laboratories, for which 16S rRNA gene Sanger sequencing of cultured isolates is frequently used. In contrast, next-generation sequencing does not require intermediate culturing steps and can be directly applied on communities, but its performance has not been extensively evaluated. We present a comparative evaluation of second (Illumina) and third (Oxford Nanopore Technologies (ONT)) generation sequencing technologies for 16S targeted genomics using a well-characterized reference sample. Different 16S gene regions were amplified and sequenced using the Illumina MiSeq, and analyzed with Mothur. Correct classification was variable, depending on the region amplified. Using a majority vote over all regions, most false positives could be eliminated at the genus level but not the species level. Alternatively, the entire 16S gene was amplified and sequenced using the ONT MinION, and analyzed with Mothur, EPI2ME, and GraphMap. Although >99% of reads were correctly classified at the genus level, up to ≈40% were misclassified at the species level. Both technologies, therefore, allow reliable identification of bacterial genera, but can potentially misguide identification of bacterial species, and constitute viable alternatives to Sanger sequencing for rapid analysis of mixed samples without requiring any culturing steps.
Melatonin Promotes Uterine and Placental Health: Potential Molecular Mechanisms
Authors: de Almeida Chuffa Luiz Gustavo , Lupi Luiz Antonio , Cucielo Maira Smaniotto , Silveira Henrique Spaulonci , Reiter Russel J. , Seiva Fábio Rodrigues Ferreira
Published: January 2020

The development of the endometrium is a cyclic event tightly regulated by hormones and growth factors to coordinate the menstrual cycle while promoting a suitable microenvironment for embryo implantation during the “receptivity window”. Many women experience uterine failures that hamper the success of conception, such as endometrium thickness, endometriosis, luteal phase defects, endometrial polyps, adenomyosis, viral infection, and even endometrial cancer; most of these disturbances involve changes in endocrine components or cell damage. The emerging evidence has proven that circadian rhythm deregulation followed by low circulating melatonin is associated with low implantation rates and difficulties to maintain pregnancy. Given that melatonin is a circadian-regulating hormone also involved in the maintenance of uterine homeostasis through regulation of numerous pathways associated with uterine receptivity and gestation, the success of female reproduction may be dependent on the levels and activity of uterine and placental melatonin. Based on the fact that irregular production of maternal and placental melatonin is related to recurrent spontaneous abortion and maternal/fetal disturbances, melatonin replacement may offer an excellent opportunity to restore normal physiological function of the affected tissues. By alleviating oxidative damage in the placenta, melatonin favors nutrient transfer and improves vascular dynamics at the uterine–placental interface. This review focuses on the main in vivo and in vitro functions of melatonin on uterine physiological processes, such as decidualization and implantation, and also on the feto-maternal tissues, and reviews how exogenous melatonin functions from a mechanistic standpoint to preserve the organ health. New insights on the potential signaling pathways whereby melatonin resists preeclampsia and endometriosis are further emphasized in this review.
Identification of Nanobodies against the Acute Myeloid Leukemia Marker CD33
Authors: Romão Ema , Krasniqi Ahmet , Maes Laila , Vandenbrande Camille , Sterckx Yann G.-J. , Stijlemans Benoit , Vincke Cécile , Devoogdt Nick , Muyldermans Serge
Published: January 2020

Nanobodies (Nbs) are the smallest antigen-binding, single domain fragments derived from heavy-chain-only antibodies from Camelidae. Among the several advantages over conventional monoclonal antibodies, their small size (12–15 kDa) allows them to extravasate rapidly, to show improved tissue penetration, and to clear rapidly from blood, which are important characteristics for cancer imaging and targeted radiotherapy. Herein, we identified Nbs against CD33, a marker for acute myeloid leukemia (AML). A total of 12 Nbs were generated against recombinant CD33 protein, out of which six bound natively CD33 protein, expressed on the surface of acute myeloid leukemia THP-1 cells. The equilibrium dissociation constants (KD) of these six Nbs and CD33 range from 4 to 270 nM, and their melting temperature (Tm) varies between 52.67 and 67.80 °C. None of these Nbs showed leukemogenicity activity in vitro. The selected six candidates were radiolabeled with 99mTc, and their biodistribution was evaluated in THP-1-tumor-bearing mice. The imaging results demonstrated the fast tumor-targeting capacity of the Nbs in vivo. Among the anti-CD33 Nbs, Nb_7 showed the highest tumor uptake (2.53 ± 0.69 % injected activity per gram (IA/g), with low background signal, except in the kidneys and bladder. Overall, Nb_7 exhibits the best characteristics to be used as an anti-CD33 targeting vehicle for future diagnostic or therapeutic applications.
Collagen/Chitosan Complexes: Preparation, Antioxidant Activity, Tyrosinase Inhibition Activity, and Melanin Synthesis
Authors: Hua Yingying , Ma Chenjun , Wei Tiantian , Zhang Liefeng , Shen Jian
Published: January 2020

Bioactive collagen/chitosan complexes were prepared by an ion crosslinking method using fish skin collagen and chitosan solution as raw materials. Scanning electron microscopy observation confirmed that the collagen/chitosan complexes were of a uniform spherical shape and uniform particle size. The complexes were stable at different pH values for a certain period of time through swelling experiments. Differential scanning calorimetry (DSC) showed the collagen/ chitosan complexes were more stable than collagen. X-ray diffraction (XRD) showed that the complexes had a strong crystal structure, and Fourier transform infrared spectroscopy (FTIR) data revealed the changes in the secondary structure of the protein due to chitosan and TPP crosslinking. The content of malondialdehyde (MDA) in the complex treatment group was considerably lower, but the content of SOD was significantly higher than that of the collagen group or chitosan group. In addition, the collagen/chitosan complexes could considerably reduce melanin content, inhibit tyrosinase activity, and down-regulate tyrosinase mRNA expression. In conclusion, the collagen/chitosan complexes were potential oral protein preparation for antioxidant enhancement and inhibiting melanin synthesis.
Rapid Fabrication of Anatomically-Shaped Bone Scaffolds Using Indirect 3D Printing and Perfusion Techniques
Authors: Grottkau Brian E. , Hui Zhixin , Yao Yang , Pang Yonggang
Published: January 2020

Fused deposit modeling (FDM) 3D printing technology cannot generate scaffolds with high porosity while maintaining good integrity, anatomical-surface detail, or high surface area-to-volume ratio (S/V). Solvent casting and particulate leaching (SCPL) technique generates scaffolds with high porosity and high S/V. However, it is challenging to generate complex-shaped scaffolds; and solvent, particle and residual water removal are time consuming. Here we report techniques surmounting these problems, successfully generating a highly porous scaffold with the anatomical-shape characteristics of a human femur by polylactic acid polymer (PLA) and PLA-hydroxyapatite (HA) casting and salt leaching. The mold is water soluble and is easily removable. By perfusing with ethanol, water, and dry air sequentially, the solvent, salt, and residual water were removed 20 fold faster than utilizing conventional methods. The porosities are uniform throughout the femoral shaped scaffold generated with PLA or PLA-HA. Both scaffolds demonstrated good biocompatibility with the pre-osteoblasts (MC3T3-E1) fully attaching to the scaffold within 8 h. The cells demonstrated high viability and proliferation throughout the entire time course. The HA-incorporated scaffolds demonstrated significantly higher compressive strength, modulus and osteoinductivity as evidenced by higher levels of alkaline-phosphatase activity and calcium deposition. When 3D printing a 3D model at 95% porosity or above, our technology preserves integrity and surface detail when compared with FDM-generated scaffolds. Our technology can also generate scaffolds with a 31 fold larger S/V than FDM. We have developed a technology that is a versatile tool in creating personalized, patient-specific bone graft scaffolds efficiently with high porosity, good scaffold integrity, high anatomical-shaped surface detail and large S/V.
Histone Deacetylase (HDAC) Gene Family in Allotetraploid Cotton and Its Diploid Progenitors: In Silico Identification, Molecular Characterization, and Gene Expression Analysis under Multiple Abiotic Stresses, DNA Damage and Phytohormone Treatments
Authors: Imran Muhammad , Shafiq Sarfraz , Naeem Muhammad Kashif , Widemann Emilie , Munir Muhammad Zeeshan , Jensen Kevin B. , Wang Richard R.-C.
Published: January 2020

Histone deacetylases (HDACs) play a significant role in a plant’s development and response to various environmental stimuli by regulating the gene transcription. However, HDACs remain unidentified in cotton. In this study, a total of 29 HDACs were identified in allotetraploid Gossypium hirsutum, while 15 and 13 HDACs were identified in Gossypium arboretum and Gossypium raimondii, respectively. Gossypium HDACs were classified into three groups (reduced potassium dependency 3 (RPD3)/HDA1, HD2-like, and Sir2-like (SRT) based on their sequences, and Gossypium HDACs within each subgroup shared a similar gene structure, conserved catalytic domains and motifs. Further analysis revealed that Gossypium HDACs were under a strong purifying selection and were unevenly distributed on their chromosomes. Gene expression data revealed that G. hirsutum HDACs were differentially expressed in various vegetative and reproductive tissues, as well as at different developmental stages of cotton fiber. Furthermore, some G. hirsutum HDACs were co-localized with quantitative trait loci (QTLs) and single-nucleotide polymorphism (SNPs) of fiber-related traits, indicating their function in fiber-related traits. We also showed that G. hirsutum HDACs were differentially regulated in response to plant hormones (abscisic acid (ABA) and auxin), DNA damage agent (methyl methanesulfonate (MMS)), and abiotic stresses (cold, salt, heavy metals and drought), indicating the functional diversity and specification of HDACs in response to developmental and environmental cues. In brief, our results provide fundamental information regarding G. hirsutum HDACs and highlight their potential functions in cotton growth, fiber development and stress adaptations, which will be helpful for devising innovative strategies for the improvement of cotton fiber and stress tolerance.
The Use of Proton Pump Inhibitors May Increase Symptoms of Muscle Function Loss in Patients with Chronic Illnesses
Authors: Vinke Paulien , Wesselink Evertine , van Orten-Luiten Wout , van Norren Klaske
Published: January 2020

Long-term use of proton pump inhibitors (PPIs) is common in patients with muscle wasting-related chronic diseases. We explored the hypothesis that the use of PPIs may contribute to a reduction in muscle mass and function in these patients. Literature indicates that a PPI-induced reduction in acidity of the gastrointestinal tract can decrease the absorption of, amongst others, magnesium. Low levels of magnesium are associated with impaired muscle function. This unwanted side-effect of PPIs on muscle function has been described in different disease backgrounds. Furthermore, magnesium is necessary for activation of vitamin D. Low vitamin D and magnesium levels together can lead to increased inflammation involved in muscle wasting. In addition, PPI use has been described to alter the microbiota’s composition in the gut, which might lead to increased inflammation. However, PPIs are often provided together with nonsteroidal anti-inflammatory drugs (NSAIDs), which are anti-inflammatory. In the presence of obesity, additional mechanisms could further contribute to muscle alterations. In conclusion, use of PPIs has been reported to contribute to muscle function loss. Whether this will add to the risk factor for development of muscle function loss in patients with chronic disease needs further investigation.
Disabling the Protease DDI2 Attenuates the Transcriptional Activity of NRF1 and Potentiates Proteasome Inhibitor Cytotoxicity
Authors: Northrop Amy , Vangala Janakiram R. , Feygin Alex , Radhakrishnan Senthil K.
Published: January 2020

Proteasome inhibition is used therapeutically to induce proteotoxic stress and trigger apoptosis in cancer cells that are highly dependent on the proteasome. As a mechanism of resistance, inhibition of the cellular proteasome induces the synthesis of new, uninhibited proteasomes to restore proteasome activity and relieve proteotoxic stress in the cell, thus evading apoptosis. This evolutionarily conserved compensatory mechanism is referred to as the proteasome-bounce back response and is orchestrated in mammalian cells by nuclear factor erythroid derived 2-related factor 1 (NRF1), a transcription factor and master regulator of proteasome subunit genes. Upon synthesis, NRF1 is cotranslationally inserted into the endoplasmic reticulum (ER), then is rapidly retrotranslocated into the cytosol and degraded by the proteasome. In contrast, during conditions of proteasome inhibition or insufficiency, NRF1 escapes degradation, is proteolytically cleaved by the aspartyl protease DNA damage inducible 1 homolog 2 (DDI2) to its active form, and enters the nucleus as an active transcription factor. Despite these insights, the cellular compartment where the proteolytic processing step occurs remains unclear. Here we further probed this pathway and found that NRF1 can be completely retrotranslocated into the cytosol where it is then cleaved and activated by DDI2. Furthermore, using a triple-negative breast cancer cell line MDA-MB-231, we investigated the therapeutic utility of attenuating DDI2 function. We found that DDI2 depletion attenuated NRF1 activation and potentiated the cytotoxic effects of the proteasome inhibitor carfilzomib. More importantly, expression of a point-mutant of DDI2 that is protease-dead recapitulated these effects. Taken together, our results provide a strong rationale for a combinational therapy that utilizes inhibition of the proteasome and the protease function of DDI2. This approach could expand the repertoire of cancer types that can be successfully treated with proteasome inhibitors in the clinic.
Development of an Improved Peroxidase-Based High-Throughput Screening for the Optimization of D-Glycerate Dehydratase Activity
Authors: Begander Benjamin , Huber Anna , Döring Manuel , Sperl Josef , Sieber Volker
Published: January 2020

Successful directed evolution examples span a broad range of improved enzyme properties. Nevertheless, the most challenging step for each single directed evolution approach is an efficient identification of improved variants from a large genetic library. Thus, the development and choice of a proper high-throughput screening is a central key for the optimization of enzymes. The detection of low enzymatic activities is especially complicated when they lead to products that are present in the metabolism of the utilized genetic host. Coupled enzymatic assays based on colorimetric products have enabled the optimization of many of such enzymes, but are susceptible to problems when applied on cell extract samples. The purpose of this study was the development of a high-throughput screening for D-glycerate dehydratase activity in cell lysates. With the aid of an automated liquid handling system, we developed a high-throughput assay that relied on a pre-treatment step of cell extract prior to performing the enzymatic and assay reactions. We could successfully apply our method, which should also be transferable to other cell extract-based peroxidase assays, to identify an improved enzyme for the dehydration of D-glycerate.
Functional Characterization of a Venom Protein Calreticulin in the Ectoparasitoid Pachycrepoideus vindemiae
Authors: Yang Lei , Wang Beibei , Qiu Liming , Wan Bin , Yang Yi , Liu Mingming , Wang Fang , Fang Qi , Stanley David W. , Ye Gongyin
Published: January 2020

Venom proteins act in the immunological interactions between parasitoids and their host insects. The effect of venom proteins on host immunity is not fully understood in pupal parasitoids. We identified the functions of a venom protein, calreticulin (PvCRT), in the pupal ectoparasitoid Pachycrepoideus vindemiae. Here, we report that PvCRT features a signal peptide and two conserved “calreticulin” domains. Multiple sequence alignments show that PvCRT shares 83.54% amino acid identity with CRT from both Pteromalus puparum and Nasonia vitripennis, which infers a close relationship among these three species. Using qPCR analysis, we found a lower expression level of PvCRT (0.27-fold) in the venom apparatus compared to the corresponding carcass. Immunohistochemical localization revealed that PvCRT was ubiquitously expressed in venom gland. The expression of the PvCRT gene in Drosophila transgenic lines via the UAS/Gal4 binary expression system reduced the self-encapsulation phenotype of tu(1)Sz1 mutants. Additionally, studies on humoral immunity indicate that PvCRT does not affect the antimicrobial immune responses of the host. This work on an ectoparasitoid will increase our understanding of venom–mediated host-parasitoid interactions.
Chronic High-Dose Neonicotinoid Exposure Decreases Overwinter Survival of Apis mellifera L.
Authors: Wood Sarah C. , Kozii Ivanna V. , Medici de Mattos Igor , de Carvalho Macedo Silva Roney , Klein Colby D. , Dvylyuk Ihor , Moshynskyy Igor , Epp Tasha , Simko Elemir
Published: January 2020

Overwinter colony mortality is an ongoing challenge for North American beekeepers. During winter, honey bee colonies rely on stored honey and beebread, which is frequently contaminated with the neonicotinoid insecticides clothianidin and thiamethoxam. To determine whether neonicotinoid exposure affects overwinter survival of Apis mellifera L., we chronically exposed overwintering field colonies and winter workers in the laboratory to thiamethoxam or clothianidin at different concentrations and monitored survival and feed consumption. We also investigated the sublethal effects of chronic thiamethoxam exposure on colony pathogen load, queen quality, and colony temperature regulation. Under field conditions, high doses of thiamethoxam significantly increased overwinter mortality compared to controls, with field-realistic doses of thiamethoxam showing no significant effect on colony overwinter survival. Under laboratory conditions, chronic neonicotinoid exposure significantly decreased survival of winter workers relative to negative control at all doses tested. Chronic high-dose thiamethoxam exposure was not shown to impact pathogen load or queen quality, and field-realistic concentrations of thiamethoxam did not affect colony temperature homeostasis. Taken together, these results demonstrate that chronic environmental neonicotinoid exposure significantly decreases survival of winter workers in the laboratory, but only chronic high-dose thiamethoxam significantly decreases overwinter survival of colonies in the field.
Hazard Mapping of Subterranean Termite Attacks in Makassar City, South Sulawesi, Indonesia
Authors: Arif Astuti , Putri Giselawati , Muin Musrizal
Published: January 2020

Termites are distributed throughout the world and often cause economic losses. This study aims to; (1) analyze the relationship between the distribution of termite species and the environmental conditions of Makassar city; (2) determine the level of attack in the Makassar City; and (3) map the risk of termite attack in the Makassar City. Samples of Pinus merkusii (2 × 2 × 20 cm) were placed at 13 observation stations and covered using polyvinyl chloride (PVC) tubes (4″ in diameter, 25-cm in length). Samples remained in place for 6 months, at which point the presence of termite attacks as well as their intensity and frequency were analyzed. Three species of termites were found among the 13 stations: Schedorhinotermes sp., Coptotermes gestroi, and Microcerotermes serrula. Their presence was significantly influenced by environmental factors at each station. The study results showed a low rate of attack in several subdistricts of Bontoala, Biring Kanaya, Makassar, Mamajang, Mariso, Manggala, Panakukang, Rappocini, Tallo, Tamalanrea, Tamalate, Ujung Pandang, Ujung Tanah, and Wajo districts. A medium level of attacks was found in subdistricts within Biring Kanaya, Manggala, Mamajang, Panakukang, Rappocini, and Tallo Districts. Finally, a high risk of termite attacks was present in several subdistricts of Biringkanaya, Tamalantera, Rappocini, Manggala, and Tamalate districts.
Electron Beam Brightness and Undulator Radiation Brilliance for a Laser Plasma Acceleration Based Free Electron Laser
Authors: Ghaith Amin , Loulergue Alexandre , Oumbarek Driss , Marcouillé Olivier , Valléau Mathieu , Labat Marie , Corde Sebastien , Couprie Marie-Emmanuelle
Published: January 2020

We report here on spontaneous undulator radiation and free electron laser calculations after a 10-m long transport line (COXINEL) using a Laser Plasma acceleration (LPA) source. The line enables the manipulation of the properties of the produced electron beams (energy spread, divergence, dispersion) in view of light source applications. The electron beam brightness and undulator radiation brilliance are addressed by an analytical approach enabling us to point out the influence of chromatic effects in the COXINEL case.
Ulcerative Colitis: Current and Emerging Treatment Strategies
Authors: Kayal Maia , Shah Shailja
Published: January 2020

Historically, medical therapy for ulcerative colitis (UC) was limited to corticosteroids. Excitingly, over the past just 1–2 decades, the options for medical therapy have expanded and include biologics and small molecules, with more agents actively being developed. In this article, we review the current and emerging treatment strategies for UC stratified according to disease severity.
Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish
Authors: Montesano Alessia , De Felice Elena , Leggieri Adele , Palladino Antonio , Lucini Carla , Scocco Paola , de Girolamo Paolo , Baumgart Mario , D’Angelo Livia
Published: January 2020

Nesfatin-1 (Nesf-1) was identified as an anorexigenic and well conserved molecule in rodents and fish. While tissue distribution of NUCB2 (Nucleobindin 2)/Nesf-1 is discretely known in vertebrates, reports on ontogenetic expression are scarce. Here, we examine the age-related central and peripheral expression of NUCB2/Nesf-1 in the teleost African turquoise killifish Nothobranchius furzeri, a consolidated model organism for aging research. We focused our analysis on brain areas responsible for the regulation of food intake and the rostral intestinal bulb, which is analogous of the mammalian stomach. We hypothesize that in our model, the stomach equivalent structure is the main source of NUCB2 mRNA, displaying higher expression levels than those observed in the brain, mainly during aging. Remarkably, its expression significantly increased in the rostral intestinal bulb compared to the brain, which is likely due to the typical anorexia of aging. When analyzing the pattern of expression, we confirmed the distribution in diencephalic areas involved in food intake regulation at all age stages. Interestingly, in the rostral bulb, NUCB2 mRNA was localized in the lining epithelium of young and old animals, while Nesf-1 immunoreactive cells were distributed in the submucosae. Taken together, our results represent a useful basis for gaining deeper knowledge regarding the mechanisms that regulate food intake during vertebrate aging.
Accuracy of Computer-Aided Dynamic Navigation Compared to Computer-Aided Static Procedure for Endodontic Access Cavities: An In Vitro Study
Authors: Zubizarreta-Macho Álvaro , de Pedro Muñoz Ana , Riad Deglow Elena , Agustín-Panadero Rubén , Mena Álvarez Jesús
Published: January 2020

Purpose: To analyze the accuracy of two computer-aided navigation techniques to guide the performance of endodontic access cavities compared with the conventional access procedure. Materials and Methods: A total of 30 single-rooted anterior teeth were selected, which were randomly distributed into three study groups: Group A—guided performance of endodontic access cavities through computer-aided static navigation system (n = 10) (SN); Group B—guided performance of endodontic access cavities through computer-aided dynamic navigation system (n = 10) (DN); and Group C—manual (freehand) performance of endodontic access cavities (n = 10) (MN). The endodontic access cavities of the SN group were performed with a stereolithography template designed on 3D implant planning software, based on preoperative cone-beam computed tomography (CBCT) and a 3D extraoral surface scan, and endodontic access cavities of the DN group were planned and performed by the dynamic navigation system. After endodontic access cavities were performed, a second CBCT was done, and the degree of accuracy between the planned and performed endodontic access cavities was analyzed using therapeutic planning software and Student’s t-test. Results: Paired t-test revealed no statistically significant differences between SN and DN at the coronal (p = 0.6542), apical (p = 0.9144), or angular (p = 0.0724) level; however, statistically significant differences were observed between the two computer-aided navigation techniques and the MN group at the coronal (p < 0.0001), apical (p < 0.0001), and angular (p < 0.0001) level. Conclusion: Both computer-aided static and dynamic navigation procedures allowed accurate performance of endodontic access cavities.
Ceruloplasmin, NT-proBNP, and Clinical Data as Risk Factors of Death or Heart Transplantation in a 1-Year Follow-Up of Heart Failure Patients
Authors: Romuk Ewa , Jacheć Wojciech , Zbrojkiewicz Ewa , Mroczek Alina , Niedziela Jacek , Gąsior Mariusz , Rozentryt Piotr , Wojciechowska Celina
Published: January 2020

We investigated whether the additional determination of ceruloplasmin (Cp) levels could improve the prognostic value of N-terminal pro-B-type natriuretic peptide (NT-proBNP) in heart failure (HF) patients in a 1-year follow-up. Cp and NT-proBNP levels and clinical and laboratory parameters were assessed simultaneously at baseline in 741 HF patients considered as possible heart transplant recipients. The primary endpoint (EP) was a composite of all-cause death (non-transplant patients) or heart transplantation during one year of follow-up. Using a cut-off value of 35.9 mg/dL for Cp and 3155 pg/mL for NT-proBNP (top interquartile range), a univariate Cox regression analysis showed that Cp (hazard ratio (HR) = 2.086; 95% confidence interval (95% CI, 1.462–2.975)), NT-proBNP (HR = 3.221; 95% CI (2.277–4.556)), and the top quartile of both Cp and NT-proBNP (HR = 4.253; 95% CI (2.795–6.471)) were all risk factors of the primary EP. The prognostic value of these biomarkers was demonstrated in a multivariate Cox regression model using the top Cp and NT-proBNP concentration quartiles combined (HR = 2.120; 95% CI (1.233–3.646)). Lower left ventricular ejection fraction, VO2max, lack of angiotensin-converting enzyme inhibitor or angiotensin receptor blocker therapy, and nonimplantation of an implantable cardioverter-defibrillator were also independent risk factors of a poor outcome. The combined evaluation of Cp and NT-proBNP had advantages over separate NT-proBNP and Cp assessment in selecting a group with a high 1-year risk. Thus multi-biomarker assessment can improve risk stratification in HF patients.
A Method to Extract Measurable Indicators of Coastal Cliff Erosion from Topographical Cliff and Beach Profiles: Application to North Norfolk and Suffolk, East England, UK
Authors: Muñoz López Pablo , Payo Andrés , Ellis Michael A. , Criado-Aldeanueva Francisco , Owen Jenkins Gareth
Published: January 2020

Recession of coastal cliffs (bluffs) is a significant problem globally, as around 80% of Earth’s coastlines are classified as sea cliffs. It has long been recognised that beaches control wave energy dissipation on the foreshore and, as a result, can provide protection from shoreline and cliff erosion. However, there have been few studies that have quantified the relationship between beach levels and cliff recession rates. One of the few quantitative studies has shown that there is a measurable relationship between the beach thickness (or beach wedge area (BWA) as a proxy for beach thickness) and the annual cliff top recession rate along the undefended coast of North Norfolk and Suffolk in eastern England, United Kingdom (UK). Additionally, previous studies also found that for profiles with low BWA, the annual cliff top recession rate frequency distribution follows a bimodal distribution. This observation suggests that as BWA increases, not only does cliff top recession rate become lower, but also more predictable, which has important implications for coastal stakeholders particularly for planning purposes at decadal and longer time scales. In this study, we have addressed some of the limitations of the previous analysis to make it more transferable to other study sites and applicable to longer time scales. In particular, we have automatised the extraction of cliff tops, toe locations, and BWA from elevation profiles. Most importantly, we have verified the basic assumption of space-for-time substitution in three different ways: (1) Extending the number or years analysed in a previous study from 11 to 24 years, (2) extending the number of locations at which cliff top recession rate and BWA are calculated, and (3) exploring the assumption of surface material remaining unchanged over time by using innovative 3D subsurface modelling. The present study contributes to our understanding of a poorly known aspect of cliff–beach interaction and outlines a quantitative approach that allows for simple analysis of widely available topographical elevation profiles, enabling the extraction of measurable indicators of coastal erosion.
Empirical Finance
Authors: Hamori Shigeyuki
Published: January 2020

The research field related to finance has made great progress in recent years due to the development of information processing technology and the availability of large-scale data. This special issue is a collection of 16 articles on empirical finance and one book review. The content is six articles on machine learning, five articles based on traditional econometric analysis, and five articles on emerging markets. The large share of articles on the application of machine learning is in line with recent trends in finance research. This special issue provides a state-of-the-art overview of empirical finance from economic, financial, and technical points of view.
Exchange Rate Regime and Economic Growth in Asia: Convergence or Divergence
Authors: Ha Dao Thi-Thieu , Hoang Nga Thi
Published: January 2020

Exchange rates and exchange rate regimes in a constantly changing economy have always attracted much attention from scholars. However, there has not been a consensus on the effect of exchange rate on economic growth. To determine the direction and magnitude of the impact of an exchange rate regime on economic growth, this study uses the exchange rate database constructed by Reinhart and Rogoff. This study also employs the GMM (Generalized Method of Moments) technique on unbalanced panel data to analyze the effect of the exchange rate regime on economic growth in Asian countries from 1994 to 2016. Empirical results suggest that a fixed exchange rate regime (weak flexibility) will affect economic growth in the same direction. As such, results from the study will serve as quantitative evidence for countries in the Asian region to consider when selecting a suitable policy and an exchange rate regime to attain high economic growth.
Short Term Effects of Revegetation on Labile Carbon and Available Nutrients of Sodic Soils in Northeast China
Authors: Yu Pujia , Tang Xuguang , Liu Shiwei , Liu Wenxin , Zhang Aichun
Published: January 2020

In response to land degradation and the decline of farmers’ income, some low quality croplands were converted to forage or grassland in Northeast China. However, it is unclear how such land use conversions influence soil nutrients. The primary objective of this study was to investigate the influences of short term conversion of cropland to alfalfa forage, monoculture Leymus chinensis grassland, monoculture Leymus chinensis grassland for hay, and successional regrowth grassland on the labile carbon and available nutrients of saline sodic soils in northeastern China. Soil labile oxidizable carbon and three soil available nutrients (available nitrogen, available phosphorus, and available potassium) were determined at the 0–50 cm depth in the five land uses. Results showed that the treatments of alfalfa forage, monoculture grassland, monoculture grassland for hay, and successional regrowth grassland increased the soil labile oxidizable carbon contents (by 32%, 28%, 15%, and 32%, respectively) and decreased the available nitrogen contents (by 15%, 19%, 34%, and 27%, respectively) in the 0–50 cm depth compared with cropland, while the differences in the contents of available phosphorus and available potassium were less pronounced. No significant differences in stratification ratios of soil labile carbon and available nutrients, the geometric means of soil labile carbon and available nutrients, and the sum scores of soil labile carbon and available nutrients were observed among the five land use treatments except the stratification ratio of 0–10/20–30 cm for available phosphorus and the values of the sum scores of soil labile carbon and available nutrients in the 0–10 cm depth. These findings suggest that short term conversions of cropland to revegetation have limited influences on the soil labile carbon and available nutrients of sodic soils in northeastern China.
Bearing Power Losses with Water-Containing Gear Fluids
Authors: Yilmaz Mustafa , Lohner Thomas , Michaelis Klaus , Stahl Karsten
Published: January 2020

Lubricants have a large influence on gearbox power losses. Recent investigations at a gear efficiency test rig have shown the high potential of water-containing gear fluids in drastically reducing load-dependent gear losses and temperatures. In this study, the bearing power losses with water-containing gear fluids were evaluated at a specific bearing power loss test rig explicitly and compared with mineral and polyalphaolefine oils. For all investigated lubricants, a Stribeck curve behavior of the load-dependent losses is observed. The water-containing gear fluids demonstrate lower no-load bearing losses and higher load-dependent bearing losses at higher rotational speeds. The comparison of measured bearing losses with typical calculation procedures showe partially large differences. The results underline the importance of having detailed knowledge of bearing losses when evaluating gear losses in gearboxes.
Effects of Rotary Swaging Parameters and Artificial Ageing on Mechanical Properties and Microstructure of 2024 Precipitation-Hardenable Aluminium Alloy
Authors: Nacházel Jan , Palán Jan , Dlouhý Jaromír , Sláma Peter , Nový Zbyšek
Published: January 2020

This work concerns mechanical properties in relation to microstructural changes in hardenable EN AW-2024 aluminium alloy in wrought and heat treated condition. The treated material benefits from synergistic effects of hardening mechanisms. Grain boundary strengthening and work hardening were activated in this material by rotary swaging. Rotary swaging is a method which shows great promise for industrial use. Precipitation hardening was achieved thanks to the material’s age hardening ability. First, the material was artificially-aged in a furnace at 140–180 °C. Second, natural ageing was used. Mechanical properties of the as-treated material were tested and microstructural processes were explored using electron microscopy and differential scanning calorimetry. The treatment route which delivered the best results was as follows: solution annealing 500 °C/1 h + water cooling + rotary swaging + artificial ageing 160 °C/21 h. This led to a yield strength close to 640 MPa, and ultimate strength above 660 MPa, and elongation of 8%. Electron backscatter diffraction observation revealed that in this condition, the ratio of Low-angle to High-angle grain boundaries is 80:20. The microstructure contains both T-phase in the interior of grains, whose particles are normally oval-shaped, and S-phase, which is present in two shapes: small oval particles or coherent needles aligned to <100> direction.
Preparation of Cu-Al/SiO2 Porous Material and Its Effect on NO Decomposition in a Cement Kiln
Authors: Gan Yanling , Cui Suping , Ma Xiaoyu , Guo Hongxia , Wang Yali
Published: January 2020

Nitrogen oxide (NOx) emissions have attracted much attention for increasing concern on the quality of the atmospheric environment. In view of NO decomposition in the cement production process, the preparation of Cu-Al/SiO2 porous material and its effect on NO decomposition were studied, and the denitrification mechanism was proposed in this paper. The NO decomposition performance of the Cu-Al/SiO2 porous material was tested via the experimental setup and infrared spectrometer and micro gas chromatography (GC). The result shows that the Cu-Al/SiO2 porous material with the template of cetyltrimethylammonium bromide (CTAB) had a better NO decomposition rate than materials with other templates when the temperature was above 500 °C, and NO decomposition rate could approach 100% at high temperatures above 750 °C. Structure analysis indicates that the prepared Cu-Al/SiO2 material structure was a mesoporous structure. The X-Ray Diffraction (XRD) and Ultraviolet–visible spectrophotometry (UV–Vis) results of the denitrification product show that the Cu-Al/SiO2 material mainly decomposed to Cu2O and Si2O, and the CuO decomposed to Cu2O and O2 at high temperature. The Cu(I)O was considered as the active phase. The redox process between Cu(II)O and Cu(I)O was thought to be the denitrification mechanism of the Cu-Al/SiO2 porous material.