bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifying Themes and Patterns on Management of Horticultural Innovations with an Automated Text Analysis
Authors: Spina Daniela , Vindigni Gabriella , Pecorino Biagio , Pappalardo Gioacchino , D’Amico Mario , Chinnici Gaetano
Published: May 2021

This research provides an overview on horticulture innovations in the last decade through a literature review and the use of a computer qualitative data analysis. We used Leximancer text mining software to identify concepts, themes and pathways linked with horticulture innovations. The software tool enabled us to “zoom out” to gain a broad perspective of the pooled data, and it indicated which studies clustered around the dominant topic. It displays the extracted information in a visual form, to wit, an interactive concept map, which summaries the interconnected themes and demonstrates any interdependencies. The text mining analysis revealed that the themes strongly related to “innovation” are “water”, “urban”, “system”, “countries” and “technology”. The outputs identified have been interpreted to discover meaning from the content analysis, since the software can facilitate a comprehensive and transparent data coding but cannot replace researcher’s interpretive work. Furthermore, we focused on the diffusion and the barriers for the spread of innovation, pointing out the differences about developing and advanced countries. This analysis allows the researcher to have a holistic understanding of the examination area and could lead to further studies.
Optimization of Agrobacterium Mediated Genetic Transformation in Paspalum scrobiculatum L. (Kodo Millet)
Authors: Bhatt Ritika , Asopa Prem Prakash , Jain Rohit , Kothari-Chajer Aditi , Kothari Shanker Lal , Kachhwaha Sumita
Published: May 2021

An efficient and reproducible protocol for Agrobacterium tumefaciens mediated genetic transformation was developed for kodo millet (Paspalum scrobiculatum L.) by optimizing various parameters. Agrobacterium strains EHA 105 and LBA 4404 harboring plasmids pCNL 56 and pCAMBIA 2300, respectively, provided the highest transformation efficiency. Addition of acetosyringone (AS) in infection medium (200 µM-EHA 105, 250 µM-LBA 4404) and co-cultivation medium (50 µM) increased the transformation efficiency. Transient and stable expression of gus gene was confirmed with histochemical assay of infected embryos and leaves of transformed plants, respectively. The best GUS response was obtained by pretreatment of callus with an antinecrotic mixture (10 mg/L Cys + 5 mg/L Ag + 2.5 mg/L As) at infection time of 20 min followed by co-cultivation for 3 days (EHA 105) and 5 days (LBA 4404) in dark. Regenerated transgenic plants were obtained after 8 to 10 weeks of selection on callus induction medium (NAA 0.5 mg/L, BAP 1 mg/L) containing 50 mg/L Kan + 250 mg/L Cef and were rooted for 2 weeks on MS medium containing PAA (1 mg/L) and phytagel. The plantlets established in greenhouse showed normal growth. Therefore, the protocol developed in the present study can be used for development of improved varieties of kodo millet.
H3K4me2 Promotes the Activation of lncCPSET1 by Jun in the Chicken PGC Formation
Authors: Zhang Chen , Zuo Qisheng , Gao Xiaomin , Hu Cai , Zhou Shujian , Chen Chen , Zou Yichen , Zhao Juanjuan , Zhang Yani , Li Bichun
Published: May 2021

Primordial germ cells are the ancestors of female and male cells. Current research has shown that long non-coding RNA (lncRNA) and Histone methylation are the pivotal epigenetic factors in the PGC formation. However, there are few studies on the regulatory mechanism of lncRNA in the formation of PGC. Here, we define the lncRNA highly expressed in chicken PGC, lncCPSET1 (chicken-PGC-specifically-expressed transcript 1) This study found that compared with the interference of lncCPSET1/histone methylase Mll2 alone, the PGC formation was severely inhibited with the interference of lncCPSET1 and histone methylase Mll2 jointly in vivo and in vitro. Studies on the transcription level of lncCPSET1 found that H3K4me2 and transcription factor Jun have a positive effect on the activation of lncCPSET1; while DNA hypomethylation inhibits the expression of lncCPSET1. In terms of mechanism, compared with DNA methylation, H3K4me2 dominates lncCPSET1 activation. H3K4me2 can be enriched in the lncCPSET1 promoter, change its chromosome conformation, recruit the transcription factor Jun, and activate the expression of lncCPSET1. Taken together, we confirmed the model that H3K4me2 rather than DNA hypomethylation mediates Jun to regulate lncCPSET1 transcription, which broadens the study of lncCPSET1 pre-transcriptional mechanism.
Effect of Post-Hatch Heat-Treatment in Heat-Stressed Transylvanian Naked Neck Chicken
Authors: Tóth Roland , Tokodyné Szabadi Nikolett , Lázár Bence , Buda Kitti , Végi Barbara , Barna Judit , Patakiné Várkonyi Eszter , Liptói Krisztina , Pain Bertrand , Gócza Elen
Published: May 2021

Although numerous studies reported the effects of heat stress in chickens, it was not investigated in the Transylvanian Naked Neck breed. In our research, Transylvanian Naked Neck chickens, 24 h after hatching, were heat-treated at 38.5 °C for 12 h. We compared the control and heat-treated adult chickens’ productivity parameters following 12 weeks of heat-stress at 30 °C. We found that the heat-treated layers had significantly higher egg production in heat stress, but in cockerels, the sperm quality did not differ significantly between the two groups. To detect the effect of heat-treatment on a molecular level, the expression of two heat-shock proteins and four heat-shock factors were analysed in the gonads of control and heat-treated chickens. We found that the expression level of HSP90 and HSF4 increased significantly in heat-treated female chicken gonads. Still, in adult females, the expression of HSF2 and HSF3 were substantially lower compared to the control. In adult heat-treated males, the HSP70, HSF1 and HSF3 expression levels showed a significant increase in both gonads compared to the control. We think that the presented significant differences in egg production might be related to the increased expression level of HSP90 and HSF4 in heat-treated female gonads.
Effect of Vitamin A Supplementation on Growth Performance, Serum Biochemical Parameters, Intestinal Immunity Response and Gut Microbiota in American Mink (Neovison vison)
Authors: Nan Weixiao , Si Huazhe , Yang Qianlong , Shi Hongpeng , Zhang Tietao , Shi Qiumei , Li Guangyu , Zhang Haihua , Liu Hanlu
Published: May 2021

This experiment investigated the effect of vitamin A supplementation on growth, serum biochemical parameters, jejunum morphology and the microbial community in male growing-furring mink. Thirty healthy male mink were randomly assigned to three treatment groups, with 10 mink per group. Each mink was housed in an individual cage. The mink in the three groups were fed diets supplemented with vitamin A acetate at dosages of 0 (CON), 20,000 (LVitA) and 1,280,000 IU/kg (HVitA) of basal diet. A 7-day pretest period preceded a formal test period of 45 days. The results show that 20,000 IU/kg vitamin A increased the ADG, serum T-AOC and GSH-Px activities, villus height and villus height/crypt depth ratio (p < 0.05). The mRNA expression levels of IL-22, Occludin and ZO-1 in the jejunum of mink were significantly higher in the LVitA group than those in the CON and HVitA groups (p < 0.05). Vitamin A supplementation increased the diversity of jejunum bacteria, decreased the ratio of Firmicutes to Bacteroidetes and increased the relative abundance of Akkermansia, uncultured bacterium f Muribaculaceae, Allobaculum, Lachnospiraceae NK4A136 group, Rummeliibacillus and Parasutterella. The comparison of potential functions also showed enrichment of glycan biosynthesis and metabolism, transport and catabolism pathways in the vitamin A supplementation groups compared with the CON group. In conclusion, these results indicate that dietary vitamin A supplementation could mediate host growth by improving intestinal development, immunity and the relative abundance of the intestinal microbiota.
The Detection of Bovine Estrus by Lactoferrin Monoclonal Antibody
Authors: Lee Jihwan , Lee Suhyun , Park Younbae , Lee Seokhyun , Ha Seungmin , Han Manhye , Jang Gulwon , Park Myunghum , Kim Kyungwoon , Chung Hakjae
Published: May 2021

To improve reproductive performance in cattle, the accurate detection of estrus and optimization of insemination relative to ovulation are necessary. However, poor heat detection by farm staff leads to a decreased conception rate, thus inflicting economic damage to the beef and dairy industries. This study aimed to develop monoclonal antibodies (mAb) that can specifically bind to the bovine lactoferrin (bLF) protein, which we have previously demonstrated to be overexpressed in bovine cervical mucus during estrus. Female rats were intraperitoneally immunized with bLF protein as the antigen. Anti-bLF mAbs were then purified by affinity chromatography, and their binding affinity for the bLF antigen was examined using ELISA. We found a high binding affinity between mAbs and bLF. Finally, we developed a rapid bovine heat detection kit using the anti-bLF mAbs that we generated and tested on cervical mucus from 12 cows (estrous synchronization, n = 2; natural cycling, n = 10). We found that the kits accurately detected estrus. Overall, our fabricated heat detection kit based on rat anti-bLF mAbs could pave the way for the development of potent tools for heat detection devices for dairy cattle, thereby preventing economic loss.
Unraveling the Relationship between Milk Yield and Quality at the Test Day with Rumination Time Recorded by a PLF Technology
Authors: Marino Rosanna , Petrera Francesca , Speroni Marisanna , Rutigliano Teresa , Galli Andrea , Abeni Fabio
Published: May 2021

The study aimed to estimate the components of rumination time (RT) variability recorded by a neck collar sensor and the relationship between RT and milk composition. Milk test day (TD) and RT data were collected from 691 cows in three farms. Daily RT data of each animal were averaged for 3, 7, and 10 days preceding the TD date (RTD). Variance component analysis of RTD, considering the effects of farm, cow, parity, TD date, and lactation phase, showed that a farm, followed by a cow, had major contributions to the total variability. The RT10 variable best performed on TD milk yield and quality records across models by a multi-model inference approach and was adopted to study its relationship with milk traits, by linear mixed models, through a 3-level stratification: low (LRT10 ≤ 8 h/day), medium (8 h/day < MRT10 ≤ 9 h/day), and high (HRT10 > 9 h/day) RT. Cows with HRT10 had greater milk, fat, protein, casein, and lactose daily yield, and lower fat, protein, casein contents, and fat to protein ratio compared to MRT10 and LRT10. Higher percentages of saturated fatty acid and lower unsaturated and monounsaturated fatty acid were found in HRT10, with respect to LRT10 and MRT10 observations.
Maternal and Neonatal Behaviour in Italian Mediterranean Buffaloes
Authors: Lanzoni Lydia , Chincarini Matteo , Giammarco Melania , Fusaro Isa , Gloria Alessia , Contri Alberto , Ferri Nicola , Vignola Giorgio
Published: May 2021

The aim of this study was to describe the neonatal and maternal behaviour of Italian Mediterranean buffaloes. Thirty primiparous buffaloes were moved into individual pens 12.5 (±2.5) days before calving. Maternal and neonatal behaviours were recorded for 48 h after calving and the analysis was performed in continuous sampling with the software BORIS. Calves’ clinical evaluations (temperature, weight, and heart and respiratory rates) were performed at different time intervals and correlated with behavioural data from the dam. Data were analysed with parametric and non-parametric methods after controlling their distribution. The maternal behavioural pattern found highlighted buffaloes’ priorities during the post-partum period: firstly, they stand and start grooming to ensure proper care for the calf; it is only after this that they dedicate time to maintenance behaviours (feeding and lying). The dams mainly groomed the calf during the first six hours after calving (average time in the 1–6-h interval: 7.7 ± 2.5 min., F = (2.5, 60.2) = 75.0; p < 0.001) to ensure the formation of the mother–infant bond; thereafter, the behaviour decreased over time. As reported in the literature, inexperienced mothers could sometimes delay the calf’s first suckling with aggressive or rejection behaviours. In this regard, 16 buffalo dams showed at least one maternal rejection behaviour, which was found to negatively correlate with calves’ daily weight gain (DWG) at 14 (rs = −0.5, p = 0.02) and 21 days (rs = −0.7, p < 0.001). The calves took on average 212.0 ± 110.0 min to suckle, and this behaviour was mainly shown during the first six hours. Overall, suckling behaviour was correlated with standing: (rs = 0.6, p < 0.001) and walking (rs = 0.9, p < 0.001). The calves’ live weight and DWG were consistently higher than the values reported in the literature. Our results present a detailed description of maternal and neonatal behaviour in the early post-partum period in Italian Mediterranean buffaloes. We also found that maternal rejection behaviours can negatively influence the calves’ growth. Finally, we think that such results can improve the management of buffaloes during the period around parturition.
Effect of Hybrid Type on Fermentation and Nutritional Parameters of Whole Plant Corn Silage
Authors: Liu Yue , Wang Guogen , Wu Hao , Meng Qingxiang , Khan Muhammad Zahoor , Zhou Zhenming
Published: May 2021

This study was designed to evaluate the effect of hybrid type on the fermentation and nutritional parameters of whole-plant corn silage (dual-purpose and silage-specific corn). For this purpose, the two corn hybrid types were harvested at the one-half to three-fourths milk line and ensiled in fermentation bags (50 × 80 cm) for 60 day. Our results demonstrated that the ratio of lactic acid to acetic acid (p = 0.004), propionic acid (p < 0.001), Flieg point (p < 0.001), ether extract (p = 0.039), starch (p < 0.001), milk-per-ton index (p < 0.005), net energy for lactation (p = 0.003), total digestible nutrients (p < 0.001), neutral detergent soluble fiber (p =0.04), and in situ dry matter digestibility (TDMDis) (p < 0.001) were higher in dual-purpose corn silage, while the pH (p = 0.014), acetic acid (p = 0.007), the ratio of ammonia nitrogen to total nitrogen (p = 0.045), neutral detergent fiber (p < 0.001), acid detergent fiber (p < 0.001), acid detergent lignin (p < 0.001), dry matter yield per ha (p < 0.001), milk-per-acre index (p = 0.003), available neutral detergent fiber (p < 0.001), and unavailable neutral detergent fiber (p < 0.001) were higher in silage-specific corn silage. Based on our analysis, we concluded that under favourable production conditions for whole-plant corn silage, the nutritive value per unit was higher in dual-purpose corn while biomass yield and nutrient value per ha were higher in silage-specific corn.
Potential Benefits to Dairy Cow Welfare of Using a Ceftiofur–Ketoprofen Combination Drug for the Treatment of Inflammatory Disease Associated with Pyrexia: A Field Clinical Trial on Acute Puerperal Metritis
Authors: Lora Isabella , Massignani Mattia , Stefani Annalisa , Gottardo Flaviana
Published: May 2021

This study aimed at investigating the benefits of using a drug combining ceftiofur and ketoprofen in a single injection on dairy cow welfare in the case of inflammatory disease with pyrexia, such as acute puerperal metritis (APM). Cows of an Italian dairy farm were examined between 5 and 14 days of calving: those with APM were randomly treated either with combined ceftiofur–ketoprofen (CD) or ceftiofur alone (C), starting from Day 0, and an equal number of healthy cows served as a control (CTR). Clinical examination and blood sampling were performed until Day 7 in each group according to specific schedules. Daily cow activity was recorded until Day 14 and daily milk production until Day 30. Additional data on fertility were collected until 120 days in milk (DIM). Data of 20 cows per group were analyzed. Body temperature and haptoglobin concentration dropped between Day 0 and 4 in both CD and C, approaching the level of CTR. The cure rate at Day 7 (body temperature < 39.0 °C) was 65 (CD) and 55% (C), without statistical difference. Neither cow activity nor milk production differed among the three groups. Reproductive performances in both CD and C were similar to CTR, but CD cows were 2.8 times more likely to be pregnant within 120 DIM than C, becoming pregnant about 14 days sooner. Both treatments (CD and C) have been effective in bringing the cows back to health conditions (CTR), and further studies would be needed to confirm the positive effect observed for CD on days open of the affected cows.
Dietary Supplementation of EGF Ameliorates the Negatively Effects of LPS on Early-Weaning Piglets: From Views of Growth Performance, Nutrient Digestibility, Microelement Absorption and Possible Mechanisms
Authors: Xue Junjing , Xie Liang , Liu Bo , Zhou Liyuan , Hu Yajun , Ajuwon Kolapo Matthew , Fang Rejun
Published: May 2021

Epidermal growth factor (EGF) plays an important role in nutrients absorption. However, whether it can be an effective additive to improve the growth performance and nutrients absorption in lipopolysaccharide (LPS) challenged early weaning piglets is still unknown. A 14-days trial was conducted to investigate how EGF attenuates the effect of LPS on the growth performance, nutrient digestibility, microelement absorption of early-weaned pigs, and study the underlying mechanism. A total of 48 early weaned piglets, aged 25 days, were randomly distributed to four groups (control, EGF, LPS and EGF + LPS groups) consisting of a 2 × 2 factorial design. The main factors were the level of LPS (HLPS = high LPS: 100 μg/kg body weight; ZLPS = low LPS: 0 μg/kg body weight) and EGF (HEGF = high EGF: 2 mg/kg diet; ZEGF = low EGF: 0 mg/kg diet). Each group had four replicates and each replicate consisted of three piglets. The results showed that piglets injected with HLPS level significantly decreased the average daily gain (ADG), and significantly increased the feed conversion ratio (FCR) compared with the piglets injected with ZLPS level, while piglets fed HEGF level significantly increased the average daily feed intake (ADFI) compared with the piglets fed ZEGF level (p < 0.05). Piglets injected with HLPS level significantly decreased the apparent digestibility of crude fat compared with the piglets injected with ZLPS level (p < 0.05). Piglets injected with HLPS level significantly increased the concentration of most microelements in the gastrointestinal tract chyme and feces, and significantly decreased the expression levels of most microelement transport-relative genes in the mucosa of gastrointestinal tissues compared with the piglets injected with ZLPS level (p < 0.05). Piglets fed HEGF level significantly decreased the concentration of microelement in the gastrointestinal tract chyme and feces, and significantly increased the expression levels of the microelement transport-relative genes in the mucosa of gastrointestinal tissues compared with the piglets fed ZEGF level (p < 0.05). In conclusion, dietary EGF could attenuate the negative effect of LPS exposure on the apparent digestibility of crude fat and microelement absorption of early-weaning piglets. EGF and LPS influenced the absorption of essential trace element through changing the expression levels of microelement transport-relative genes in the mucosa of gastrointestinal tissues. In the early weaning piglets, EGF can be used as an additive to increase the essential trace elements absorption.
D-Penicillamine: The State of the Art in Humans and in Dogs from a Pharmacological and Regulatory Perspective
Authors: Pugliese Michela , Biondi Vito , Gugliandolo Enrico , Licata Patrizia , Peritore Alessio Filippo , Crupi Rosalia , Passantino Annamaria
Published: May 2021

Chelant agents are the mainstay of treatment in copper-associated hepatitis in humans, where D-penicillamine is the chelant agent of first choice. In veterinary medicine, the use of D-penicillamine has increased with the recent recognition of copper-associated hepatopathies that occur in several breeds of dogs. Although the different regulatory authorities in the world (United States Food and Drugs Administration—U.S. FDA, European Medicines Agency—EMEA, etc.) do not approve D-penicillamine for use in dogs, it has been used to treat copper-associated hepatitis in dogs since the 1970s, and is prescribed legally by veterinarians as an extra-label drug to treat this disease and alleviate suffering. The present study aims to: (a) address the pharmacological features; (b) outline the clinical scenario underlying the increased interest in D-penicillamine by overviewing the evolution of its main therapeutic goals in humans and dogs; and finally, (c) provide a discussion on its use and prescription in veterinary medicine from a regulatory perspective.
ATP Bioluminescence for Rapid and Selective Detection of Bacteria and Yeasts in Wine
Authors: Monica Saverio , Bancalari Elena , Castellone Vincenzo , Rijkx Jos , Wirth Sarah , Jahns Anika , Bottari Benedetta
Published: May 2021

Microbial contamination may represent a loss of money for wine producers as several defects can arise due to a microorganism’s growth during storage. The aim of this study was to implement a bioluminescence assay protocol to rapidly and simultaneously detect bacteria and yeasts in wines. Different wines samples were deliberately contaminated with bacteria and yeasts at different concentrations and filtered through two serial filters with decreasing mesh to separate bacteria and yeasts. These were resuscitated over 24 h on selective liquid media and analyzed by bioluminescence assay. ATP measurements discriminated the presence of yeasts and bacteria in artificially contaminated wine samples down to 50 CFU/L of yeasts and 1000 CFU/L of bacteria. The developed protocol allowed to detect, rapidly (24 h) and simultaneously, bacteria and yeasts in different types of wines. This would be of great interest for industries, for which an early detection and discrimination of microbial contaminants would help in the decision-making process.
A Novel Software Architecture Solution with a Focus on Long-Term IoT Device Security Support
Authors: Dodig Ivica , Cafuta Davor , Kramberger Tin , Cesar Ivan
Published: May 2021

This paper presents a solution for upgrading a previous device model to an Industry 4.0 smart device, with the goal of maintaining high compatibility. A novel IoT architecture is presented that satisfies the characteristics of a smart device. We analysed existing IoT architectures and proposed a new architecture to achieve long-term security and usability. To ensure long-term security, we eliminated the possibility of device configuration outside the immediate vicinity of the device with a dedicated protocol. The security concepts of the existing architectures were also analysed and further modified. To improve compatibility with previous device models, we propose a new method to collect data from sensors by introducing a multithreaded microcontroller. We propose additional software components to ensure factory programming, maintenance, and cloud Big Data analysis. Based on our experiments, we adapted the algorithm to increase the accuracy of the temperature and flow sensors by using a temperature calibration device and known flow cycles. Measurement results are presented to confirm the successful upgrade. We designed a hardware architecture to ensure compatibility with previous and future device models. Issues with previous sensors encountered during the upgrade were discussed and resolved. A novel software architecture based on security for long-term IoT devices is proposed.
A Feedrate Planning Method for the NURBS Curve in CNC Machining Based on the Critical Constraint Curve
Authors: Guo Peng , Wu Yijie , Yang Guang , Shen Zhebin , Zhang Haorong , Zhang Peng , Lou Fei , Li Hengbo
Published: May 2021

The curvature of the NURBS curve varies along its trajectory, therefore, the commonly used feedrate-planning method, which based on the acceleration/deceleration (Acc/Dec) model, is difficult to be directly applied in CNC machining of a NURBS curve. To address this problem, a feedrate-planning method based on the critical constraint curve of the feedrate (CCC) is proposed. Firstly, the problems of existing feedrate-planning methods and their causes are analyzed. Secondly, by considering both the curvature constraint and the kinematic constraint during the Acc/Dec process, the concept of CCC which represents the relationship between the critical feedrate-constraint value and the arc length is proposed. Then the CCC of a NURBS curve is constructed, and it has a concise expression conforming to the Acc/Dec model. Finally, a feedrate-planning method of a NURBS curve based on CCC and the Acc/Dec model is established. In the simulation, a comparison between the proposed method and the conventional feedrate-planning method is performed, and the results show that, the proposed method can reduce the Acc/Dec time by over 40%, while little computational burden being added. The machining experimental results validate the real-time performance and stability of the proposed method, and also the machining quality is verified. The proposed method offers an effective feedrate-planning strategy for a NURBS curve in CNC machining.
Effect of Muscle-Specific Fatigue on the Risk of Anterior Cruciate Ligament Injury in Females
Authors: Moon Jeheon , Lee Jinseok , Kim Keehyun , Koo Dohoon , Lee Jusung , Pathak Prabhat , Sanchez Gustavo Adrian Ruiz , Panday Siddhartha Bikram
Published: May 2021

The aim of our study was to investigate the effect of muscle-specific fatigue of the quadriceps and hamstring muscles on the biomechanical factors of anterior cruciate ligament (ACL) injury using musculoskeletal modeling techniques during directional diversion maneuver. Fifteen female subjects performed a directional diversion maneuver under three treatment conditions (quadriceps fatigue, hamstring fatigue, and control gait). Data from the 3D motion capture system and force platform were used to extract anterior/posterior ACL forces using the two-bundle ACL musculoskeletal modeling approach. A decrease in maximum extension (51.3%) and flexion (50.7%) torque after fatigue was observed. After quadriceps fatigue, the extension (p = 0.041) and adduction moments (p = 0.046) of the knee joint and the mean anterior bundle of ACL force (p = 0.021) decreased significantly. The knee flexion angle (p = 0.003), knee valgus angle (p = 0.013), and shear force (p = 0.043) decreased significantly after hamstring fatigue. The decrease in ACL force after quadriceps fatigue confirms its significant role in causing an ACL injury. However, no significant differences in ACL load after hamstring fatigue leads us to speculate that the antagonist muscle group, i.e., the hamstring, might not have a preventive mechanism against ACL injury.
Optimization of the Configuration of the Power Sections of Special Small-Sized Positive Displacement Motors for Deep-Penetrating Perforation Using the Technical System “Perfobore”
Authors: Liagov Ilia , Liagov Alexander , Liagova Anastasiia
Published: May 2021

Innovative technologies for the secondary drilling of productive reservoirs, based on the multihole drilling deeply remoted from the wellbore and with controlled trajectories, to overcome the contamination of the pay zone, and with the ability to carry out various geological and technological wellworks many times over, can significantly increase the efficiency of the development of old and marginal wells. To implement such tasks, it is necessary to use special technological drilling equipment, one such technical system (TS) is the Perfobore system, which uses a modular construction. The methodology allows to choose an optimal variant for the power sections with a high-torque, small-sized positive displacement motor PDM, previously developed by specialists at the Perfobore company. This made it possible to design universal sectional motors with diameters of 43 to 54 mm with improved characteristics compared to serial PDMs. The production of prototypes of multi-section PDMs and their successful bench and then field tests (FT), from 2018 to 2020, in the Uralopovolzhskaya and West Siberian oil and gas provinces, as part of the TS Perfobore, proved the correctness of the chosen technical strategy.
Rough Dental Implant Surfaces and Peri-Implantitis: Role of Phase-Contrast Microscopy, Laser Protocols, and Modified Home Oral Hygiene in Maintenance. A 10-Year Retrospective Study
Authors: Caccianiga Gianluigi , Rey Gérard , Caccianiga Paolo , Leonida Alessandro , Baldoni Marco , Baldoni Alessandro , Ceraulo Saverio
Published: May 2021

The aim of this study was to evaluate two different kinds of rough implant surface and to assess their tendency to peri-implantitis disease, with a follow-up of more than 10 years. Data were obtained from a cluster of 500 implants with Ti-Unite surface and 1000 implants with Ossean surface, with a minimum follow-up of 10 years. Implants had been inserted both in pristine bone and regenerated bone. We registered incidence of peri-implantitis and other causes of implant loss. All patients agreed with the following maintenance protocol: sonic brush with vertical movement (Broxo), interdental brushes, and oral irrigators (Broxo) at least two times every day. For all patients with implants, we evaluated subgingival plaque samples by phase-contrast microscopy every 4 months for a period of more than 10-years. Ti-Unite surface implants underwent peri-implantitis in 1.6% of the total number of implants inserted and Ossean surface implants showed peri-implantitis in 1.5% of the total number of implants. The total percentage of implant lost was 4% for Ti-Unite surfaces and 3.6% for Ossean surfaces. Strict control of implants leads to low percentage of peri-implantitis even for rough surfaces dental implants.
Influence of Shock Wave on Loss and Breakdown of Tip-Leakage Vortex in Turbine Rotor with Varying Backpressure
Authors: Wei Zuojun , Ren Guangming , Gan Xiaohua , Ni Ming , Chen Weijie
Published: May 2021

In modern turbine rotors, tip-leakage flow is a common phenomenon that accounts for about 1/3 of the stage loss. Studies show that as the imposed load increases, a shock wave appears in the tip region, which causes a significant interference on the leakage vortex. In the present study, numerical simulations are carried out to investigate the influence of the shock wave on the loss and breakdown of the tip-leakage vortex. The obtained results indicate that with no effective control on the flow, the loss of the leakage vortex has an approximate exponential growth up to about 10 times as the outlet Mach number increases from 0.67 to 1.15 and the corresponding proportion in the total loss increases sharply to 30.2%. It is found that the stagnation position of the breakdown changes with the backpressure and the amplitude of variation along the axial direction is up to 0.13 Cx. It is inferred that the breakdown of the leakage vortex core may be affected by the periodical passing of downstream blade and the induced pressure fluctuation may result in additional vibration in this rotor blade. The leakage vortex is unstable in supersonic flow with a shock wave and it may transfer to a flow with a low-velocity bubble in its core region. It is concluded that the leakage vortex breakdown mainly originates from interferences of the shock wave, while the internal cause of such breakdown is the centrifugal instability of the vortex.
A Hybrid Gearbox Fault Diagnosis Method Based on GWO-VMD and DE-KELM
Authors: Yao Gang , Wang Yunce , Benbouzid Mohamed , Ait-Ahmed Mourad
Published: May 2021

In this paper, a vibration signal-based hybrid diagnostic method, including vibration signal adaptive decomposition, vibration signal reconstruction, fault feature extraction, and gearbox fault classification, is proposed to realize fault diagnosis of general gearboxes. The main contribution of the proposed method is the combining of signal processing, machine learning, and optimization techniques to effectively eliminate noise contained in vibration signals and to achieve high diagnostic accuracy. Firstly, in the study of vibration signal preprocessing and fault feature extraction, to reduce the impact of noise and mode mixing problems on the accuracy of fault classification, Variational Mode Decomposition (VMD) was adopted to realize adaptive signal decomposition and Wolf Grey Optimizer (GWO) was applied to optimize parameters of VMD. The correlation coefficient was subsequently used to select highly correlated Intrinsic Mode Functions (IMFs) to reconstruct the vibration signals. With these re-constructed signals, fault features were extracted by calculating their time domain parameters, energies, and permutation entropies. Secondly, in the study of fault classification, Kernel Extreme Learning Machine (KELM) was adopted and Differential Evolutionary (DE) was applied to search its regularization coefficient and kernel parameter to further improve classification accuracy. Finally, gearbox vibration signals in healthy and faulty conditions were obtained and contrast experiences were conducted to validate the effectiveness of the proposed hybrid fault diagnosis method.
Real Color Model of a Cadaver for Deep Brain Stimulation of the Subthalamic Nucleus
Authors: Kim Chung-Yoh , Park Jin-Seo , Chung Beom-Sun
Published: May 2021

When performing deep brain stimulation (DBS) of the subthalamic nucleus, practitioners should interpret the magnetic resonance images (MRI) correctly so they can place the DBS electrode accurately at the target without damaging the other structures. The aim of this study is to provide a real color volume model of a cadaver head that would help medical students and practitioners to better understand the sectional anatomy of DBS surgery. Sectioned images of a cadaver head were reconstructed into a real color volume model with a voxel size of 0.5 mm × 0.5 mm × 0.5 mm. According to preoperative MRIs and postoperative computed tomographys (CT) of 31 patients, a virtual DBS electrode was rendered on the volume model of a cadaver. The volume model was sectioned at the classical and oblique planes to produce real color images. In addition, segmented images of a cadaver head were formed into volume models. On the classical and oblique planes, the anatomical structures around the course of the DBS electrode were identified. The entry point, waypoint, target point, and nearby structures where the DBS electrode could be misplaced were also elucidated. The oblique planes could be understood concretely by comparing the volume model of the sectioned images with that of the segmented images. The real color and high resolution of the volume model enabled observations of minute structures even on the oblique planes. The volume models can be downloaded by users to be correlated with other patients’ data for grasping the anatomical orientation.
Optimal Control for a Hydraulic Recuperation System Using Endoreversible Thermodynamics
Authors: Masser Robin , Hoffmann Karl Heinz
Published: May 2021

Energy savings in the traffic sector are of considerable importance for economic and environmental considerations. Recuperation of mechanical energy in commercial vehicles can contribute to this goal. One promising technology rests on hydraulic systems, in particular for trucks which use such system also for other purposes such as lifting cargo or operating a crane. In this work the potential for energy savings is analyzed for commercial vehicles with tipper bodies, as these already have a hydraulic onboard system. The recuperation system is modeled based on endoreversible thermodynamics, thus providing a framework in which realistic driving data can be incorporated. We further used dissipative engine setups for modeling both the hydraulic and combustion engine of the hybrid drive train in order to include realistic efficiency maps. As a result, reduction in fuel consumption of up to 26% as compared to a simple baseline recuperation strategy can be achieved with an optimized recuperation control.
Unveiling the Reaction Mechanism of the Das/Chechik/Marek Synthesis of Stereodefined Quaternary Carbon Centers
Authors: Silva Pedro J. , Bernardo Carlos E. P.
Published: May 2021

The reaction mechanism of the Cu+-catalyzed introduction of two all-carbon-substituted stereocenters in an ynamide system using a Grignard reagent, a zinc carbenoid, and an aldehyde, was investigated using density-functional theory. In contrast to the formation of an organocopper(I) compound and subsequent carbocupration reaction, previously postulated as the initial step, the reaction proved to instead proceed through an initial complexation of the substrate alkyne bond by the Cu+-catalyst, which primes this bond for reaction with the Grignard reagent. Subsequent addition of the zinc carbenoid then enables the nucleophilic attack on the incoming aldehyde, which is revealed as the rate-limiting step. Our computations have also identified the factors governing the regio- and setereoselectivity of this interesting reaction, and suggest possible paths for its further development.
A Watermarking Scheme for Color Image Using Quaternion Discrete Fourier Transform and Tensor Decomposition
Authors: Li Li , Bai Rui , Lu Jianfeng , Zhang Shanqing , Chang Ching-Chun
Published: May 2021

To protect the copyright of the color image, a color image watermarking scheme based on quaternion discrete Fourier transform (QDFT) and tensor decomposition (TD) is presented. Specifically, the cover image is partitioned into non-overlapping blocks, and then QDFT is performed on each image block. Then, the three imaginary frequency components of QDFT are used to construct a third-order tensor. The third-order tensor is decomposed by Tucker decomposition and generates a core tensor. Finally, an improved odd–even quantization technique is employed to embed a watermark in the core tensor. Moreover, pseudo-Zernike moments and multiple output least squares support vector regression (MLS–SVR) network model are used for geometric distortion correction in the watermark extraction stage. The scheme utilizes the inherent correlations among the three RGB channels of a color image, and spreads the watermark into the three channels. The experimental results indicate that the proposed scheme has better fidelity and stronger robustness for common image-processing and geometric attacks, can effectively resist each color channel exchange attack. Compared with the existing schemes, the presented scheme achieves better performance.
Application of Electrocoagulation with a New Steel-Swarf-Based Electrode for the Removal of Heavy Metals and Total Coliforms from Sanitary Landfill Leachate
Authors: de Oliveira Mayk Teles , Torres Ieda Maria Sapateiro , Ruggeri Humberto , Scalize Paulo , Albuquerque Antonio , Gil Eric de Souza
Published: May 2021

Sanitary landfill leachate (LL) composition varies according to climate variables variation, solid waste characteristics and composition, and landfill age. Leachate treatment is essentially carried out trough biological and physicochemical processes, which have showed variability in efficiency and appear a costly solution for the management authorities. Electrocoagulation (EC) seems a suitable solution for leachate treatment taking into account the characteristics of the liquor. One of the problems of EC is the electrode passivation, which affects the longevity of the process. One solution to this problem could be the replacement of the electrode by one made of recyclable material, which would make it possible to change it frequently and at a lower cost. The objective of the present work was to evaluate the removal of heavy metals (As, Ba, Cd, Cr, Cu, Fe, Pb, Mn, Ni, Se and Zn) and coliforms from a LL by EC using electrodes made from steel swarf (SfE) up to 8 h. Removal efficiencies of detected heavy metals were 51%(Cr), 59%(As), 71%(Cd), 72%(Zn), 92%(Ba), 95%(Ni) and >99%(Pb). The microbial load of coliforms in leachate was reduced from 10.76 × 104 CFU/mL (raw leachate) to less than 1 CFU/mL (after treatment with SfE) (i.e., approximately 100% reduction). The use of SfE in EC of LL is very effective in removing heavy metals and coliforms and can be used as alternative treatment solution for such effluents.
Protein Expression Level Changes in Weissella koreensis during Garlic Media Fermentation
Authors: Park Youn-Jin , Jang Myoung-Jun
Published: May 2021

This study investigated the changes in Weissella koreensis (WK) protein expression levels during fermentation in MRS medium supplemented with garlic of WK. WK was first discovered as lactic acid bacteria (LAB) in a Korean fermented cabbage dish known as kimchi. The number of WK cells in MRS medium with garlic (MBCG) and without (MB) after 7 days was 3.55 × 1010 and 2.55 × 1010 CFU/mL, respectively. To observe the changes in the carbon sources in the media, we measured the glucose, sucrose, lactic acid, and acetic acid levels in each medium (MB and MBCG). Thus, 67.2 ± 2.4 (MB) and 64.2 ± 4.7 (MBCG) mmol−1 of glucose were consumed. For sucrose, the level was 3.5 ± 2.2 (MB), and 3.4 ± 2.5 (MBCG) mmol−1. There was not much difference in the lactic acid and acetic acid levels at 160.8 ± 0.4 (MB) and 159.2 ± 0.2 (MBCG) and 2.4 ± 0.4 (MB) and 2.2 ± 8.1 (MBCG) mmol−1, respectively. After the 7-day fermentation period, two-dimensional electrophoresis (2DE) was used to confirm the protein expression pattern in the WK strain. The results show that the fusA and ssb1 proteins were reduced, and the clpP protein was increased. Afterwards, the expression patterns of the above proteins were confirmed through qRT-PCR. Thus, this study confirms the changes in protein expression levels in Weissella koreensis when garlic was added to the media. This study provides basic data for future studies on the major biosynthetic pathways of WK.
Entomopathogenic Fungi and Bacteria in a Veterinary Perspective
Authors: Ebani Valentina Virginia , Mancianti Francesca
Published: May 2021

The present study aimed to review the papers dealing with the biological activity of fungi and bacteria against some mites and ticks of veterinary interest. In particular, the attention was turned to the research regarding acarid species, Dermanyssus gallinae and Psoroptes sp., which are the cause of severe threat in farm animals and, regarding ticks, also pets. Their impact on animal and human health has been stressed, examining the weaknesses and strengths of conventional treatments. Bacillus thuringiensis, Beauveria bassiana and Metarhizium anisopliae are the most widely employed agents. Their activities have been reviewed, considering the feasibility of an in-field application and the effectiveness of the administration alone or combined with conventional and alternative drugs is reported.
Clinical Assessment of Endothelial Function in Convalescent COVID-19 Patients Undergoing Multidisciplinary Pulmonary Rehabilitation
Authors: Ambrosino Pasquale , Molino Antonio , Calcaterra Ilenia , Formisano Roberto , Stufano Silvia , Spedicato Giorgio Alfredo , Motta Andrea , Papa Antimo , Di Minno Matteo Nicola Dario , Maniscalco Mauro
Published: May 2021

Background: Growing evidence points to a key role of endothelial dysfunction in the pathogenesis of COVID-19. In this study, we evaluated changes in endothelium-dependent flow-mediated dilation (FMD) in a cohort of convalescent COVID-19 patients undergoing pulmonary rehabilitation (PR). Methods: After swab test negativization, convalescent COVID-19 patients referring to a post-acute care facility for PR were consecutively screened for inclusion. Study procedures were performed at the time of hospitalization and discharge. Results: We enrolled 82 convalescent COVID-19 patients (85.4% males, mean age 60.4 years). After PR, a significant improvement in most pulmonary function tests and exercise capacity was documented. FMD changed from 2.48% ± 2.01 to 4.24% ± 2.81 (p < 0.001), corresponding to a 70.9% increase. Significantly higher changes in FMD were found in patients without a history of vascular events as compared to those with (+2.04% ± 2.30 vs. +0.61% ± 1.83, p = 0.013). Values of forced expiratory volume in 1 s (FEV1%), forced vital capacity (FVC%) and diffusion capacity for carbon monoxide (DLCO%) significantly and directly correlated with FMD both at baseline and after PR. Patients with normal FEV1% (≥80% predicted) during the overall study period or those normalizing FEV1% after PR showed a more significant FMD change as compared to patients with persistently impaired FEV1% (<80% predicted) (p for trend = 0.029). This finding was confirmed in a multivariate analysis. Conclusions: Clinically evaluated endothelial function improves after PR in convalescent COVID-19 patients. A direct and persistent association between the severity of pulmonary and vascular disease can be hypothesized. Endothelial function testing may be useful in the follow-up of convalescent COVID-19 patients.
In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis
Authors: Huang Shang-En , Sulistyowati Erna , Chao Yu-Ying , Wu Bin-Nan , Dai Zen-Kong , Hsu Jong-Hau , Yeh Jwu-Lai
Published: May 2021

Osteoarthritis is a degenerative arthropathy that is mainly characterized by dysregulation of inflammatory responses. KMUP-1, a derived chemical synthetic of xanthine, has been shown to have anti-inflammatory and antioxidant properties. Here, we aimed to investigate the in vitro anti-inflammatory and in vivo anti-osteoarthritis effects of KMUP-1. Protein and gene expressions of inflammation markers were determined by ELISA, Western blotting and microarray, respectively. RAW264.7 mouse macrophages were cultured and pretreated with KMUP-1 (1, 5, 10 μM). The productions of TNF-α, IL-6, MMP-2 and MMP- 9 were reduced by KMUP-1 pretreatment in LPS-induced inflammation of RAW264.7 cells. The expressions of iNOS, TNF-α, COX-2, MMP-2 and MMP-9 were also inhibited by KMUP-1 pretreatment. The gene expression levels of TNF and COX families were also downregulated. In addition, KMUP-1 suppressed the activations of ERK, JNK and p38 as well as phosphorylation of IκBα/NF-κB signaling pathways. Furthermore, SIRT1 inhibitor attenuated the inhibitory effect of KMUP-1 in LPS-induced NF-κB activation. In vivo study showed that KMUP-1 reduced mechanical hyperalgesia in monoiodoacetic acid (MIA)-induced rats OA. Additionally, KMUP-1 pretreatment reduced the serum levels of TNF-α and IL-6 in MIA-injected rats. Moreover, macroscopic and histological observation showed that KMUP-1 reduced articular cartilage erosion in rats. Our results demonstrated that KMUP-1 inhibited the inflammatory responses and restored SIRT1 in vitro, alleviated joint-related pain and cartilage destruction in vivo. Taken together, KMUP-1 has the potential to improve MIA-induced articular cartilage degradation by inhibiting the levels and expression of inflammatory mediators suggesting that KMUP-1 might be a potential therapeutic agent for OA.