bluuebunny/crossref_metadata_2025 huggingface dataset:
Post-COVID-19 Pain Is Not Associated with DNA Methylation Levels of the ACE2 Promoter in COVID-19 Survivors Hospitalized Due to SARS-CoV-2 Infection
Authors: Fernández-de-las-Peñas César , Díaz-Gil Gema , Gil-Crujera Antonio , Gómez-Sánchez Stella M. , Ambite-Quesada Silvia , Franco-Moreno Anabel , Ryan-Murua Pablo , Torres-Macho Juan , Pellicer-Valero Oscar J. , Arendt-Nielsen Lars , Giordano Rocco
Published: July 2024

One of theories explaining the development of long-lasting symptoms after an acute severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection include changes in the methylation pattern of the host. The current study aimed to investigate whether DNA methylation levels associated with the angiotensin-converting enzyme 2 (ACE2) promoter are different when comparing individuals previously hospitalized due to COVID-19 who then developed long-lasting post-COVID pain with those previously hospitalized due to COVID-19 who did not develop post-COVID-19 pain symptoms. Non-stimulated saliva samples were obtained from a cohort of 279 (mean age: 56.5, SD: 13.0 years old, 51.5% male) COVID-19 survivors who needed hospitalization. Clinical data were collected from hospital medical records. Participants were asked to disclose pain symptoms developed during the first three months after hospital admission due to COVID-19 and persisting at the time of the interview. Methylations of five CpG dinucleotides in the ACE2 promoter were quantified (as percentages). Participants were evaluated up to 17.8 (SD: 5.3) months after hospitalization. Thus, 39.1% of patients exhibited post-COVID-19 pain. Most patients (77.05%) in the cohort developed localized post-COVID-19 pain. Headache and pain in the lower extremity were experienced by 29.4% of the patients. Seven patients received a post-infection diagnosis of fibromyalgia based on the presence of widespread pain characteristics (11.6%) and other associated symptoms. No significant differences in methylation percentages at any CpG location of the ACE2 promoter were identified when comparing individuals with and without post-COVID-19 pain. The current study did not observe differences in methylation levels of the ACE2 promoter depending on the presence or absence of long-lasting post-COVID-19 pain symptoms in individuals who needed hospitalization due to COVID-19 during the first wave of the pandemic.
Combined Effects of Fibroblast Growth Factor-2 and Carbonate Apatite Granules on Periodontal Healing: An In Vivo and In Vitro Study
Authors: Miyata Naoki , Mori Shinta , Murakami Tasuku , Bizenjima Takahiro , Seshima Fumi , Imamura Kentaro , Saito Atsushi
Published: July 2024

The aim of this study was to investigate in vivo and in vitro the effectiveness of the use of fibroblast growth factor (FGF)-2 with carbonate apatite (CO3Ap) on periodontal healing. Periodontal defects created in the maxillary first molars in rats were treated with FGF-2, CO3Ap, FGF-2 + CO3Ap or left unfilled. Healing was evaluated using microcomputed tomography, histological, and immunohistochemical analyses. In vitro experiments were performed to assess cellular behaviors and the expression of osteoblastic differentiation markers in MC3T3-E1 cells. At 4 weeks, the bone volume fraction in the FGF-2 + CO3Ap group was significantly greater than that in the CO3Ap group, but there was no significant difference from the FGF-2 group. The FGF-2 + CO3Ap group demonstrated greater new bone compared with the FGF-2 or CO3Ap group. The FGF-2 + CO3Ap group showed greater levels of osteocalcin-positive cells compared with the CO3Ap group, but there was no significant difference from the FGF-2 group. In vitro, the FGF-2 + CO3Ap group exhibited a greater extent of cell attachment and more elongated cells compared with the CO3Ap group. Compared with the CO3Ap group, the FGF-2 + CO3Ap group showed significantly higher viability/proliferation, but the expressions of Runx2 and Sp7 were reduced. The results indicated that the use of FGF-2 with CO3Ap enhanced healing in the periodontal defects. FGF-2 promoted cell attachment to and proliferation on CO3Ap and regulated osteoblastic differentiation, thereby contributing to novel bone formation.
Decompressive Hemicraniectomy without Evacuation of Acute Intraparenchymal Hemorrhage
Authors: Blanco-Acevedo Cristóbal , Aguera-Morales Eduardo , Fuentes-Fayos Antonio C. , Pelaez-Viña Nazareth , Diaz-Pernalete Rosa , Infante-Santos Nazaret , Muñoz-Jurado Ana , Porras-Pantojo Manuel F. , Ibáñez-Costa Alejandro , Luque Raúl M. , Solivera-Vela Juan
Published: July 2024

Background: Intracerebral hemorrhages (ICHs) are prevalent, with high morbidity and mortality. We analyzed whether decompressive craniectomy (DC) without evacuation of the acute intraparenchymal hematoma could produce better functional outcomes than treatment with evacuation. Methods: Patients with acute ICH treated with DC without clot evacuation, or evacuation with or without associated craniectomy were included. Matched univariate analyses were performed, and a binary logistic regression model was constructed using the Glasgow Outcome Scale (GOS) and modified Rankin scale (mRS) as dependent variables. Results: 27 patients treated with DC without clot evacuation were compared to 36 patients with clot evacuation; eleven of the first group were matched with 18 patients with evacuation. A significantly better functional prognosis in the group treated with DC without clot evacuation was found. Patients aged < 55 years and treated with DC without clot evacuation had a significantly better functional prognosis (p = 0.008 and p = 0.039, respectively). In multivariate analysis, the intervention performed was the greatest predictor of functional status at the end of follow-up. Conclusions: DC without clot evacuation improves the functional prognosis of patients with acute intraparenchymal hematomas. Larger multicenter studies are warranted to determine whether a change in the management of acute ICH should be recommended.
Thyroid Nodule Characterization: Overview and State of the Art of Diagnosis with Recent Developments, from Imaging to Molecular Diagnosis and Artificial Intelligence
Authors: David Emanuele , Grazhdani Hektor , Tattaresu Giuliana , Pittari Alessandra , Foti Pietro Valerio , Palmucci Stefano , Spatola Corrado , Lo Greco Maria Chiara , Inì Corrado , Tiralongo Francesco , Castiglione Davide , Mastroeni Giampiero , Gigli Silvia , Basile Antonio
Published: July 2024

Ultrasound (US) is the primary tool for evaluating patients with thyroid nodules, and the risk of malignancy assessed is based on US features. These features help determine which patients require fine-needle aspiration (FNA) biopsy. Classification systems for US features have been developed to facilitate efficient interpretation, reporting, and communication of thyroid US findings. These systems have been validated by numerous studies and are reviewed in this article. Additionally, this overview provides a comprehensive description of the clinical and laboratory evaluation of patients with thyroid nodules, various imaging modalities, grayscale US features, color Doppler US, contrast-enhanced US (CEUS), US elastography, FNA biopsy assessment, and the recent introduction of molecular testing. The potential of artificial intelligence in thyroid US is also discussed.
Human–Exoskeleton Coupling Simulation for Lifting Tasks with Shoulder, Spine, and Knee-Joint Powered Exoskeletons
Authors: Arefeen Asif , Xia Ting , Xiang Yujiang
Published: July 2024

In this study, we introduce a two-dimensional (2D) human skeletal model coupled with knee, spine, and shoulder exoskeletons. The primary purpose of this model is to predict the optimal lifting motion and provide torque support from the exoskeleton through the utilization of inverse dynamics optimization. The kinematics and dynamics of the human model are expressed using the Denavit–Hartenberg (DH) representation. The lifting optimization formulation integrates the electromechanical dynamics of the DC motors in the exoskeletons of the knee, spine, and shoulder. The design variables for this study include human joint angle profiles and exoskeleton motor current profiles. The optimization objective is to minimize the squared normalized human joint torques, subject to physical and task-specific lifting constraints. We solve this optimization problem using the gradient-based optimizer SNOPT. Our results include a comparison of predicted human joint angle profiles, joint torque profiles, and ground reaction force (GRF) profiles between lifting tasks with and without exoskeleton assistance. We also explore various combinations of exoskeletons for the knee, spine, and shoulder. By resolving the lifting optimization problems, we designed the optimal torques for the exoskeletons located at the knee, spine, and shoulder. It was found that the support from the exoskeletons substantially lowers the torque levels in human joints. Additionally, we conducted experiments only on the knee exoskeleton. Experimental data indicated that using the knee exoskeleton decreases the muscle activation peaks by 35.00%, 10.03%, 22.12%, 30.14%, 16.77%, and 25.71% for muscles of the erector spinae, latissimus dorsi, vastus medialis, vastus lateralis, rectus femoris, and biceps femoris, respectively.
Enhanced Nanoparticle Recognition via Deep Learning-Accelerated Plasmonic Sensing
Authors: Jin Ke-Xin , Shen Jia , Wang Yi-Jing , Yang Yu , Cao Shuo-Hui
Published: July 2024

Surface plasmon microscopy proves to be a potent tool for capturing interferometric scattering imaging data of individual particles at both micro and nanoscales, offering considerable potential for label-free analysis of bio-particles and bio-molecules such as exosomes, viruses, and bacteria. However, the manual analysis of acquired images remains a challenge, particularly when dealing with dense samples or strong background noise, common in practical measurements. Manual analysis is not only prone to errors but is also time-consuming, especially when handling a large volume of experimental images. Currently, automated methods for sensing and analysis of such data are lacking. In this paper, we develop an accelerated approach for surface plasmon microscopy imaging of individual particles based on combining the interference scattering model of single particle and deep learning processing. We create hybrid datasets by combining the theoretical simulation of particle images with the actual measurements. Subsequently, we construct a neural network utilizing the EfficientNet architecture. Our results demonstrate the effectiveness of this novel deep learning technique in classifying interferometric scattering images and identifying multiple particles under noisy conditions. This advancement paves the way for practical bio-applications through efficient automated particle analysis.
Enhanced Stability and Detection Range of Microbial Electrochemical Biotoxicity Sensor by Polydopamine Encapsulation
Authors: Guan Zengfu , Yan Jiaguo , Yan Haiyuan , Li Bin , Guo Lei , Sun Qiang , Geng Tie , Guo Xiaoxuan , Liu Lidong , Yan Wenqing , Wang Xin
Published: July 2024

With the rapid development of modern industry, it is urgently needed to measure the biotoxicity of complex chemicals. Microbial electrochemical biotoxicity sensors are an attractive technology; however, their application is usually limited by their stability and reusability after measurements. Here, we improve their performance by encapsulating the electroactive biofilm with polydopamine (PDA), and we evaluate the improvement by different concentrations of heavy metal ions (Cu2+, Ag+, and Fe3+) in terms of inhibition ratio (IR) and durability. Results indicate that the PDA-encapsulated sensor exhibits a more significant detection concentration than the control group, with a 3-fold increase for Cu2+ and a 1.5-fold increase for Ag+. Moreover, it achieves 15 more continuous toxicity tests than the control group, maintaining high electrochemical activity even after continuous toxicity impacts. Images from a confocal laser scanning microscope reveal that the PDA encapsulation protects the activity of the electroactive biofilm. The study, thus, demonstrates that PDA encapsulation is efficacious in improving the performance of microbial electrochemical biotoxicity sensors, which can extend its application to more complex media.
Effect of Spatial and Temporal Prediction on Tactile Sensitivity
Authors: Kunimura Hiroshi , Oda Hitoshi , Kawasaki Taku , Gao Han , Fukuda Shiho , Hiraoka Koichi
Published: July 2024

The purpose of the present study was to examine whether spatial or temporal prediction of the tactile stimulus contributes to tactile sensitivity. To investigate the effect of spatial prediction on tactile sensitivity, electrical stimuli were provided for the digit nerve in one of five fingers, and advanced notice of the stimulating finger was provided before the stimulus in some trials but not in others. There was no significant effect of spatial prediction on the intensity at the perceptual threshold of the digit nerve stimulus. This indicates that spatial prediction of the tactile stimulus does not influence tactile sensitivity. To examine the effect of temporal prediction, an auditory warning cue was provided 0, 1, or 10 s before the electrical stimulus to the digit nerve. The stimulus intensity at the perceptual threshold in the trials with the 1 s warning cue was lower than those with the 0 s warning cue. This indicates that temporal prediction enhances tactile sensitivity. The stimulus intensity at the perceptual threshold in the trials with the 1 s warning cue was lower than those with the 10 s warning cue. This means that the contribution of temporal prediction to the tactile sensitivity is greater as the warning cue is closer to the time of the stimulus. This finding may be explained by a defense mechanism activated when humans predict that a tactile stimulus is coming soon.
Neural Dynamics of Processing Inflectional Morphology: An fMRI Study on Korean Inflected Verbs
Authors: Kim Joonwoo , Kim Sangyub , Nam Kichun
Published: July 2024

The present study aimed to elucidate the neural mechanisms underpinning the visual recognition of morphologically complex verbs in Korean, a morphologically rich, agglutinative language with inherent polymorphemic characteristics. In an fMRI experiment with a lexical decision paradigm, we investigated whether verb inflection types (base, regular, and irregular) are processed through separate mechanisms or a single system. Furthermore, we explored the semantic influence in processing inflectional morphology by manipulating the semantic ambiguity (homonymous vs. unambiguous) of inflected verbs. The results showed equivalent activation levels in the left inferior frontal gyrus for both regular and irregular verbs, challenging the dichotomy between the two. Graded effects of verb regularity were observed in the occipitotemporal regions, with regular inflections eliciting increased activation in the fusiform and lingual gyri. In the middle occipital gyrus, homonyms showed decreased activation relative to that of unambiguous words, specifically for base and irregular forms. Furthermore, the angular gyrus exhibited significant modulation with all verb types, indicating a semantic influence during morphological processing. These findings support single-system theories and the connectionist framework, challenging the assumptions of purely orthographic morphological decomposition and dual-mechanism accounts. Furthermore, they provide evidence for a semantic influence during morphological processing, with differential reliance on semantic activation for regular and irregular inflections.
“All You Need Is Love” a Social Network Approach to Understanding Attachment Networks in Adulthood
Authors: Tian Junnan , Freeman Harry
Published: July 2024

This study examined five dimensions of attachment network structure in a large sample of adults (n = 930, 57% female) between 24 to 80 years of age. We employed a newly validated diagrammatic measure, the web-based hierarchical mapping technique (WHMT), to measure the attachment strength to participants’ five closest relationships and the physical distance to and between network members. Our first aim was to replicate existing research on the composition of adult attachment networks, exploring variations in network patterns by age, romantic status, and parental status. Our second aim was to explore four new network dimensions, including physical distance to network members, hierarchical patterns, centrality, and density. The results replicated previous work on network composition, highlighting the pivotal role of romantic partners as primary attachment figures through adulthood. The analysis of the new network dimensions revealed a clear divide between adults in romantic relationships and those who are not. Compared to the single adults, the adults in romantic relationships were more hierarchical in their attachment preferences, reported lower emotional connection to friends and parents, and lived farther from their network, which was also more geographically dispersed. In other words, romantically involved adults put more of their attachment eggs in one basket. The results also showed that the older adults tended to live further away from their attachment network and had a more geographically dispersed network compared to the younger adults.
Study on Seismic Behavior of Cross-Shaped-Steel-Reinforced RPC Columns
Authors: Wang Jingmin , Zhu Zhiyu , Wang Kun
Published: July 2024

Based on the hysteretic tests of steel-reinforced reactive powder concrete (RPC) columns and reinforced RPC columns, the finite element numerical models of these two kinds of RPC columns were established by OpenSees (2016). The feasibility of the model was verified by comparing the results of tests and simulation. On this basis, the nonlinear analysis of seismic performance of cross-shaped-steel-reinforced RPC columns was carried out. The influences of different factors such as longitudinal reinforcement ratio, steel sectional resistance moment, RPC grade, steel strength and section form of shape steel on the hysteretic performance were investigated. Finally, the hysteretic model of cross-shaped-steel-reinforced RPC columns was established. The results showed that, compared with H-shaped-steel-reinforced RPC columns, the peak bearing capacity of cross-shaped-steel-reinforced RPC columns was increased by 21.2%, but the displacement ductility was obviously reduced. With the increase of slenderness ratio, the lateral stiffness and horizontal bearing capacity of cross-shaped steel RPC columns decreased rapidly. In addition, the peak load was improved with the increase of RPC strength, steel sectional resistance moment and longitudinal reinforcement ratio. The hysteretic model was consistent with the simulation results, which can effectively predict the hysteretic characteristics of cross-shaped-steel-reinforced RPC columns. The research results can provide a theoretical basis for the engineering design and application of cross-shaped-steel-reinforced RPC columns.
Mitigating Making-Do Practices Using the Last Planner System and BIM: A System Dynamic Analysis
Authors: Karaz Mahmoud , Teixeira José Manuel Cardoso , Amaral Tatiana Gondim do
Published: July 2024

Effective waste elimination is critical for the success of construction projects. Although several studies have focused on various aspects of construction waste, limited efforts have yet to investigate the dynamic effect of Making-Do (MD) practices on productivity, rework, defects, and material waste. From a lean construction perspective, this study aims to address MD waste using the Last Planner System (LPS) and BIM. First, the causal structure that can cause MD in construction projects was expressed in a causal loop diagram (CLD), and thematic analysis uncovered the strategies of LPS-BIM to eliminate MD identified by reviewing the literature. Secondly, twenty-five strategies from the LPS and BIM strategies to address MD using structural equation modeling (SEM) were assessed. Subsequently, a system dynamics model (SDM) for investigating LPS-BIM strategies on MD decisions in a construction project was formulated based on the underlying causal loop diagrams and the mathematical relations among the variables. Finally, the model was applied to three projects, and simulations for four LPS-BIM scenarios were carried out. The findings show that dynamic interactions among diverse production planning and control factors are critical in evaluating MD impacts on a construction project. The results demonstrate that the LPS-BIM approach resulted in an average 43.8% reduction in the tasks performed with MD, 45.3% of constraints, 66.5% of construction waste, an increasing 13.7% completion rate, and a 29.3% cost reduction, demonstrating that LPS-BIM is a more efficient solution for MD mitigation and construction planning. This study aims to guide construction planners and policymakers to better manage their production constraints by eliminating negative MD practices from their plans.
Retrospective Analysis of Efficacy and Toxicity of Stereotactic Body Radiotherapy and Surgical Resection of Adrenal Metastases from Solid Tumors
Authors: Lütscher Jamie , Gelpke Hans , Zehnder Adrian , Mauti Laetitia , Padevit Christian , John Hubert , Batifi Nidar , Zwahlen Daniel Rudolf , Förster Robert , Schröder Christina
Published: July 2024

Background: This single-center retrospective study aimed to evaluate the efficacy and toxicity profiles of stereotactic body radiotherapy (SBRT) and surgical resection in patients with adrenal metastases originating from solid tumors. Methods/Materials: Patients with advanced tumor conditions or comorbidities typically received SBRT, whereas those considered physically fit underwent standard surgical treatment. Endpoints included local control (LC), progression free survival (PFS), overall survival (OS), and complication rates (CR). Results: 41 patients with 48 adrenal metastases were included, with 27 (65.9%) patients receiving SBRT and 14 (34.1%) patients undergoing adrenalectomy. One- and two-year LC values were 100% for both periods after adrenalectomy, and 70.0% and 52.5% after SBRT (p = 0.001). PFS showed values of 40.2% and 32.1% at one and two years after adrenalectomy and of 10.6% for both periods after SBRT (p = 0.223). OS was 83.3% both one and two years after surgery and 67.0% and 40.2% after SBRT (p = 0.031). There was no statistically significant difference between the two groups regarding acute complications (p = 0.123). Conclusion: Despite potential confounders, adrenalectomy exhibited statistically significant superior LC and OS compared to SBRT in managing adrenal metastases, while both treatment methods displayed acceptable toxicity profiles. However, patient selection bias must be taken into account when directly comparing the two therapy modalities. Nevertheless, the study provides new and important results for the scientific and medical communities regarding oncological outcomes after SBRT or surgical resection of adrenal metastases.
Enhanced Lung Cancer Detection Using a Combined Ratio of Antigen–Autoantibody Immune Complexes against CYFRA 21-1 and p53
Authors: Kim Heyjin , Lee Jin Kyung , Kim Hye-Ryoun , Hong Young Jun
Published: July 2024

The early detection of lung cancer (LC) improves patient outcomes, but current methods have limitations. Autoantibodies against tumor-associated antigens have potential as early biomarkers. This study evaluated the 9G testTM Cancer/Lung, measuring circulating complexes of two antigen–autoantibody immune complexes (AIC) against their respective free antigens (CYFRA 21-1 and p53) for LC diagnosis. We analyzed 100 LC patients and 119 healthy controls using the 9G testTM Cancer/Lung, quantifying the levels of AICs (CYFRA 21-1-Anti-CYFRA 21-1 autoantibody immune complex (CIC) and p53-Anti-p53 autoantibody immune complex (PIC)), free antigens (CYFRA 21-1 and p53), and ratios of AICs/antigens (LC index). The levels of the CICs and PICs were significantly elevated in LC compared to the controls (p < 0.0062 and p < 0.0026), while free antigens showed no significant difference. The CIC/CYFRA 21-1 and PIC/p53 ratios were also significantly higher in LC (all, p < 0.0001). The LC index, when combining both ratios, exhibited the best diagnostic performance with an area under the curve (AUC) of 0.945, exceeding individual CICs, PICs, and free antigens (AUCs ≤ 0.887). At a cut-off of 3.60, the LC index achieved 81% sensitivity and 95% specificity for LC diagnosis. It detected early-stage (Stage I–II) LC with 87.5% sensitivity, exceeding its performance in advanced stages (72.7%). The LC index showed no significant differences based on age, gender, smoking status (former, current, or never smoker), or pack years smoked. The LC index demonstrates promising potential for early LC diagnosis, exceeding conventional free antigen markers.
Analysis of Molecular Imaging and Laboratory Baseline Biomarkers in PSMA-RLT: Whole-Body Total Lesion PSMA (TLP) Predicts Overall Survival
Authors: Hein Connor , Burgard Caroline , Blickle Arne , Bastian Moritz B. , Maus Stephan , Schaefer-Schuler Andrea , Hoffmann Manuela A. , Schreckenberger Mathias , Ezziddin Samer , Rosar Florian
Published: July 2024

The aim of this retrospective study was to identify pre-therapeutic predictive laboratory and molecular imaging biomarkers for response and overall survival (OS) in patients with metastatic castration-resistant prostate cancer (mCRPC) treated with prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT). Pre-therapeutic laboratory and [68Ga]Ga-PSMA-11 PET/CT data of n = 102 mCRPC patients receiving [177Lu]Lu-PSMA-617 RLT within a prospective registry (REALITY Study, NCT04833517) were analyzed including laboratory parameters such as alkaline phosphatase (ALP), prostate-specific antigen (PSA), gamma glutamyl transferase (GGT), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), neuron specific enolase (NSE), hemoglobin (Hb), and imaging parameters such as maximum standardized uptake value of the tumor lesions (SUVmax), the mean standardized uptake value of all tumor lesions (SUVmean), the whole-body molecular tumor volume (MTV), and the whole-body total lesion PSMA (TLP). Mann–Whitney U test, univariate and multivariable Cox-regression were performed to test for association of the parameters with response and OS. The SUVmean of all lesions was significantly different between responders and non-responders (SUVmean responders 8.95 ± 2.83 vs. non-responders 7.88 ± 4.46, p = 0.003), whereas all other tested biochemical and imaging parameters did not reveal significant differences. Hb and the molecular imaging parameters MTV and TLP showed a significant association with OS (p = 0.013, p = 0.005; p = 0.009) in univariant Cox regression; however, only TLP remained significant in multivariable analysis (Hazard ratio 1.033, p = 0.009). This study demonstrates a statistically significant association between the quantitative PET/CT imaging parameter SUVmean and PSA response, as well as between the baseline TLP and OS of mCRPC patients undergoing RLT.
The Antidepressant Action of Fluoxetine Involves the Inhibition of Dlx5/6 in Cortical GABAergic Neurons through a TrkB-Dependent Pathway
Authors: Aouci Rym , Fontaine Anastasia , Vion Amïn , Belz Lou , Levi Giovanni , Narboux-Nême Nicolas
Published: July 2024

Major depressive disorder (MDD) is a complex and devastating illness that affects people of all ages. Despite the large use of antidepressants in current medical practice, neither their mechanisms of action nor the aetiology of MDD are completely understood. Experimental evidence supports the involvement of Parvalbumin-positive GABAergic neurons (PV-neurons) in the pathogenesis of MDD. DLX5 and DLX6 (DLX5/6) encode two homeodomain transcription factors involved in cortical GABAergic differentiation and function. In the mouse, the level of expression of these genes is correlated with the cortical density of PV-neurons and with anxiety-like behaviours. The same genomic region generates the lncRNA DLX6-AS1, which, in humans, participates in the GABAergic regulatory module downregulated in schizophrenia and ASD. Here, we show that the expression levels of Dlx5/6 in the adult mouse brain are correlated with the immobility time in the forced swim test, which is used to measure depressive-like behaviours. We show that the administration of the antidepressant fluoxetine (Flx) to normal mice induces, within 24 h, a rapid and stable reduction in Dlx5, Dlx6 and Dlx6-AS1 expression in the cerebral cortex through the activation of the TrkB-CREB pathway. Experimental Dlx5 overexpression counteracts the antidepressant effects induced by Flx treatment. Our findings show that one of the short-term effects of Flx administration is the reduction in Dlx5/6 expression in GABAergic neurons, which, in turn, has direct consequences on PV expression and on behavioural profiles. Variants in the DLX5/6 regulatory network could be implicated in the predisposition to depression and in the variability of patients’ response to antidepressant treatment.
Changes in Smartphone Usage among Adolescents and Associated Subjective Health Concerns: A Secondary Analysis of the Korea Youth Risk Behavior Survey
Authors: Lee Geun Woo , Moon Jongwon , Lee Donghun
Published: July 2024

Background: We evaluated changes in the smartphone use rate and time among Korean adolescents and their awareness of associated health problems. Methods: This study was a secondary analysis of the Korea Youth Risk Behavior Survey (2020–2023) conducted by the Korean Disease Control and Prevention Agency. The total number of enrolled adolescents aged 12–18 years was 214,526. Results: The weekly smartphone usage rate increased from 96.4% to 97.1% (p = 0.03), with no significant changes observed in weekend usage. The average smartphone use time was 4.7 h on weekdays (p = 0.17) and 6.6 h on weekends (p = 0.37). Middle school adolescents had a higher weekday use rate than high school adolescents, but the average smartphone use time was significantly less. By 2023, the proportion of adolescents with overdependence was 28% (n = 14,672). Additionally, 11.8% (n = 6255) responded that they had experienced health problems due to smartphone use. Conclusions: The longer they used their smartphones for on the weekends, the more likely they considered their health to be worse. In conclusion, our youth population needs to be educated on the proper use of smartphones.
Biologics and Small Molecule Targeted Therapies for Pediatric Alopecia Areata, Psoriasis, Atopic Dermatitis, and Hidradenitis Suppurativa in the US: A Narrative Review
Authors: Yi Robin C. , Moran Shannon K. , Gantz Hannah Y. , Strowd Lindsay C. , Feldman Steven R.
Published: July 2024

Background: The management of pediatric dermatological conditions such as alopecia areata (AA), psoriasis, atopic dermatitis (AD), and hidradenitis suppurativa (HS) has significantly evolved with the introduction of biologics and small molecule targeted therapies. The advancement in understanding the immunopathogenesis of these chronic skin conditions has led to the development and approval of novel biologics and small molecule therapies. Initially approved by the United States Food and Drug Administration (FDA) for adults, most of these therapies are now being evaluated in clinical trials for safety and efficacy in adolescents and children, expanding new treatment options for pediatric patients. The role of the FDA in drug approval is multifaceted from drug inception, ensuring that research, data, and evidence show that the proposed drug is effective and safe for the intended use. Objective: The goal of this review article is to provide an overview of the recently FDA-approved and potential biologic and oral small molecule therapies in clinical trials for AA, psoriasis, AD, and HS in pediatric patients. Methods: The search for this review included keywords in ClinicalTrials.gov, PubMed, and Google Scholar for the latest research and clinical trials relevant to these conditions and treatments without the PRISMA methodology. Results: For pediatric AA, ritlecitinib is FDA-approved, while baricitinib and updacitinib are in phase 3 clinical trials for pediatric approval. The FDA-approved drugs for pediatric psoriasis include secukinumab, ustekinumab, ixekizumab, etanercept, and apremilast. Other phase 3 clinical trials for pediatric psoriasis include risankizumab, guselkumab, tildrakizumab, brodalumab, and deucravacitinib. For pediatric AD, the FDA-approved drugs are dupilumab, tralokinumab, abrocitinib, and upadacitinib, with many other drugs in phase 3 trials. Adalimumab is an FDA-approved biologic for pediatric HS, with various clinical trials ongoing for adults. The approved biologics and small molecule therapies had higher efficacy and improved safety profiles compared to traditional medications. Conclusions: With numerous ongoing trials, the success of these clinical trials could lead to their inclusion in treatment guidelines for these chronic skin conditions. Biologics and small molecule therapies offer new avenues for effective disease management, enabling personalized therapeutic interventions and improving pediatric health outcomes.
Relations between Neurocognitive Function and Visual Acuity: A Cross-Sessional Study in a Cohort of Premature Children
Authors: Tu Chun-Hsien , Wu Wei-Chi , Chin Wei-Chih , Hsu Shih-Chieh , Tang I , Hsu Jen-Fu , Chou Hung-Da , Kang Eugene Yu-Chuan , Huang Yu-Shu
Published: July 2024

Background: Premature children with retinopathy of prematurity (ROP) have been reported to an have increased risk of visual and neurocognitive impairments, yet little is known about whether vision could affect specific neurocognition. This study aimed to clarify the correlations between neurocognition and vision in premature children. Materials and Methods: This is a nonrandomized, cross-sectional, observational study in a pediatric cohort with five groups: (1) full-term (n = 25), (2) prematurity without ROP (n = 154), (3) prematurity with ROP but without treatment (n = 39), (4) prematurity with ROP and with bevacizumab (IVB) treatment (n = 62), and (5) prematurity with ROP and with laser/laser + IVB treatment (n = 20). Neurocognitive function was evaluated by the Wechsler Preschool and Primary Scale of Intelligence, Fourth Edition (WPPSI-IV) around the age of 4 years. Visual acuity (VA) and refractive errors were tested. Correlations between WPPSI parameters and visual outcomes were analyzed across five groups. Results: Among the 300 recruited children (mean age = 4.02 + 0.97 years, male = 56.3%), 297 were assessed by WPPSI-IV and 142 were assessed by vision tests. The Full-Scale Intelligence Quotient (FSIQ) index was worse in the premature groups. After adjusting for covariates, seven items, including FSIQ-Index (p = 0.047), fluid-reasoning index (p = 0.004), FR-percentile ranking (p = 0.008), object assembly (p = 0.034), picture concept (p = 0.034), zoo locations (p = 0.014) and bug search (p = 0.020), showed significant differences between groups. The better the best corrected VA (BCVA), the higher the scores on Verbal Comprehension Index (VCI), VCI-PR, and the subtest of information. Conclusions: Specific cognitive dysfunctions are related to the BCVA in this large cohort. Subtest performance profiles in WPPSI can be affected by prematurity, ROP treatment, and different ROP treatment. FSIQ is generally lower in premature children and even lower in children with ROP.
Self-Healing and Thermal Stability of LaMgAl11O19-Ti3AlC2 Composites for High-Temperature Abradable Applications
Authors: Huang Jingqi , Chen Wenbo , Lü Kaiyue , Xu Mingyi , Deng Longhui , Jiang Jianing , Dong Shujuan , Chen Meizhu , Cao Xueqiang
Published: July 2024

Defects such as interconnected pores and cracks can improve the abradability of ceramic-based abradable sealing coatings (ASCs) but may reduce the lifetime. Self-healing can potentially close cracks and transform interconnected pores into isolated ones through filling and sintering effects. Ti3AlC2 (TAC) was incorporated into LaMgAl11O19 (LMA) as both the self-healing agent and sintering aid, and plasma-sprayed LMA-based composite coatings were annealed at 1200 °C to assess their self-healing capabilities and then subjected to oxidation in air and corrosion in steam at 1300 °C to study their long-term stability. Results indicated that increasing TAC content significantly enhances self-healing effectiveness, evidenced by the closure of cracks and the isolation of pores. Oxidation and corrosion at 1300 °C led to significant grain growth and the formation of equiaxed grains with an aspect ratio of approximately 3, which may impair the toughening mechanism. Meanwhile, due to the preferential volatilization of Al in a steam environment, LTA decomposed into α-La2/3TiO3 and La4Ti3O12 phases, and the accelerated mass transfer also resulted in grain coarsening. Interestingly, the L20T composite coating with a porosity of 32.17 ± 0.94% and a hardness of 74.88 ± 1.55 HR15Y showed great potential for abradable applications due to its stable phase composition and uniform pore distribution.
Stereotactic Body Radiation Therapy for Symptomatic Pancreatic Insulinoma: Two-Case Report and Literature Review
Authors: Namysl-Kaletka Agnieszka , Wydmanski Jerzy , Debosz-Suwinska Iwona , Kaszuba Malgorzata , Gabrys Dorota , Roch-Zniszczol Agata , Handkiewicz-Junak Daria
Published: July 2024

Insulinoma is the most common functional neuroendocrine tumor of the pancreas, with the main clinical symptom being hypoglycemia. The standard treatment is surgery, but some patients are not eligible for surgery, while in those operated on, the risk of perioperative complications is up to 30%. Diazoxide treatment to prevent hypoglycemia is effective only in 50% of patients. To prevent tumor growth and hormonal excess, stereotactic radiotherapy may be an alternative to surgical treatment. In our paper, we present two cases of patients with insulinoma treated successfully with stereotactic body radiation therapy (SBRT).
Oxygen Concentration and Its Implications for Microbial Structure and Metabolism: A Case Study in a Deep Tropical Reservoir
Authors: Del’Duca Alessandro , Janiques Amanda Meirelles de Sá , Azevedo Raiza dos Santos , Roland Fábio , Cesar Dionéia Evangelista
Published: July 2024

The vertical stratification of oxygen concentration in deep reservoirs impacts nutrient cycling and ecosystem biodiversity. The Serra da Mesa reservoir, the largest in Brazil, was studied to evaluate the structure and production of the prokaryote community at five depths. Using 3H leucine incorporation and fluorescent in situ hybridization (FISH), the study focused on different depths near the dam, particularly within the euphotic zone. The water column was characterized into oxic, transitional, and hypoxic layers based on dissolved oxygen concentration. The highest densities and biomasses of prokaryotes were found at the euphotic zone’s depth limit, where bacterial production was low, suggesting inactive or slow-growing bacteria. Cell size differences and filamentous bacteria presence near the surface were observed, likely due to varying predation pressures. Prokaryote community composition differed across depths. At the subsurface level, with high dissolved organic carbon, alphaproteobacteria, betaproteobacteria, and Cytophaga–Flavobacter had similar densities, but the lowest bacterial biomass was recorded. The highest dissolved oxygen concentration depth had the lowest bacterial density, dominated by alphaproteobacteria and gammaproteobacteria. The study revealed that prokaryotic community structure and production vary with depth, indicating that microbial participation in layer dynamics is differentiated, with variations in abundance and distribution linked to oxygen concentrations.
SparrKULee: A Speech-Evoked Auditory Response Repository from KU Leuven, Containing the EEG of 85 Participants
Authors: Accou Bernd , Bollens Lies , Gillis Marlies , Verheijen Wendy , Van hamme Hugo , Francart Tom
Published: July 2024

Researchers investigating the neural mechanisms underlying speech perception often employ electroencephalography (EEG) to record brain activity while participants listen to spoken language. The high temporal resolution of EEG enables the study of neural responses to fast and dynamic speech signals. Previous studies have successfully extracted speech characteristics from EEG data and, conversely, predicted EEG activity from speech features. Machine learning techniques are generally employed to construct encoding and decoding models, which necessitate a substantial quantity of data. We present SparrKULee, a Speech-evoked Auditory Repository of EEG data, measured at KU Leuven, comprising 64-channel EEG recordings from 85 young individuals with normal hearing, each of whom listened to 90–150 min of natural speech. This dataset is more extensive than any currently available dataset in terms of both the number of participants and the quantity of data per participant. It is suitable for training larger machine learning models. We evaluate the dataset using linear and state-of-the-art non-linear models in a speech encoding/decoding and match/mismatch paradigm, providing benchmark scores for future research.
Design of an Innovative Twin-Disc Device for the Evaluation of Wheel and Rail Profile Wear
Authors: Magelli Matteo , Pagano Rosario , Zampieri Nicolò
Published: July 2024

The tribological properties of steels used to realise railway wheels play a fundamental role in the performances of both vehicle and infrastructure. In particular, the wear process, caused by the wheel–rail interaction, modifies the shape of wheel and rail profiles, changing the performances of the vehicle. For this reason, research institutes and vehicle manufacturers have worked hard to develop predictive tools able to estimate the evolution of the wheel and rail profiles. The efficiency of these tools is strongly influenced by the tribological properties of the materials, i.e., the wear coefficients, which are used as input data. The characterisation of these properties requires specific tools and long-lasting experimental campaigns, which are usually performed under controlled operating conditions, using twin-disc test benches. These devices usually do not consider the real contact conditions in terms of normal load, contact geometry, and slip velocity, since they are equipped with small-size rollers. The paper proposes an innovative 1:5 scaled twin-disc, which allows the reproduction of the real wheel–rail contact conditions, thanks to Pascal’s scaling technique. The testing device allows the reproduction of a wide range of typical operating conditions of railway vehicles, thanks to high-power independent brushless motors, used to actuate the rollers, and an innovative loading system.
Peripherical Blood hsa-miR-335-5p Quantification as a Prognostic, but Not Diagnostic, Marker of Gastric Cancer
Authors: Ramírez-Vidal Lizbeth , Becerril-Rico Jared , Monroy-Mora Alberto , Tinajero-Rodríguez Jose Manuel , Centeno-Cruz Federico , Oñate-Ocaña Luis F. , Ortiz-Sánchez Elizabeth
Published: July 2024

Gastric cancer (GC) is a leading cause of death, and this pathology often receives a diagnosis in an advanced stage. The development of a less invasive and cost-effective test for detection is essential for decreasing the mortality rate and increasing the life expectancy of GC patients. We evaluated the potential targeting of CD54/ICAM1, a marker of gastric cancer stem cells, with miRNAs to detect GC in blood samples. The analyses included 79 blood samples, 38 from GC patients and 41 from healthy donors, who attended INCan, México City. The total RNA was obtained from the blood plasma, and RT-PCR and qPCR were performed to obtain the relative expression of each miRNA. Hsa-miR-335-5p was detected in the plasma of GC patients and healthy donors at the same levels. The ROC curve analyses indicated that this miRNA was not a candidate for the molecular diagnosis of GC. We did not observe a correlation between the expression of hsa-miR-335-5p and clinical variables; however, the Kaplan–Meier analyses indicated that, in patients who survived more than 12 months, a lower expression of hsa-miR-335-5p was correlated with a better prognosis. It would be convenient to evaluate a larger panel of miRNAs, including miRNAs expressed in a limited number of cell types or with a low number targets, to obtain more specific candidates for developing a robust test for the diagnosis/prognosis of GC.
The Auditory Steady-State Response and the Relationship between Electrophysiological and Behavioural Thresholds
Authors: Luiz Cyntia Barbosa Laureano , Gil Daniela , Skarzynski Piotr Henryk , Skarżyńska Magdalena Beata , Sanfins Milaine Dominici , Azevedo Marisa Frasson de
Published: July 2024

Background: This study examined the relationship between behavioural thresholds as measured by pure tone audiometry and electrophysiological thresholds measured by the Auditory Steady-State Response (ASSR) in children with normal hearing and sensorineural hearing loss. Materials and Methods: After being assessed, 45 children of both sexes, ranging in age from 5 to 15, were split into four groups: 10 with moderate to moderately severe sensorineural hearing loss (G2M); 10 with steeply sloping sensorineural hearing loss (G2D); 10 with profound and severe sensorineural hearing loss (G2S); and 15 with normal hearing (G1). ASSR, tympanometry, acoustic reflex testing, pure tone audiometry, and speech audiometry (SRT and SDT) were performed. Results: The electrophysiological maximum in the group with normal hearing thresholds varied from 19 to 27 dB NA. The correlation in the group with moderate to moderately severe hearing loss was 0.42–0.74. The correlation in the steeply sloping hearing loss group was 0.68–0.94. The correlation in the group of people with profound and severe hearing loss was 0.59–0.86. The normal hearing group’s mean differences in ASSR threshold and audiometric threshold ranged from −0.3 to 12 dB, in the moderate and moderately severe hearing loss group from −9 to 2 dB, in the steeply sloping hearing loss group from 1.4 to 7.5 dB, and in the severe and profound hearing loss group from −0.40 to 8.5 dB. Conclusion: As expected, there was no strong relationship between behavioural and electrophysiological thresholds in the group with normal hearing. But in children with hearing loss, there was a strong correlation between electrophysiological and behavioural thresholds; this relationship was especially evident in children with severe and profound hearing loss and those with steeply sloping hearing loss.