bluuebunny/crossref_metadata_2025 huggingface dataset:
Early Extra-Uterine Growth Restriction in Very-Low-Birth-Weight Neonates with Normal or Mildly Abnormal Brain MRI: Effects on a 2–3-Year Neurodevelopmental Outcome
Authors: Massirio Paolo , Battaglini Marcella , Bonato Irene , De Crescenzo Sara , Calevo Maria Grazia , Malova Mariya , Caruggi Samuele , Parodi Alessandro , Preiti Deborah , Zoia Agata , Uccella Sara , Tortora Domenico , Severino Mariasavina , Rossi Andrea , Traggiai Cristina , Nobili Lino , Striano Pasquale , Ramenghi Luca Antonio
Published: February 2024

Extra-uterine growth restriction (EUGR) is a common complication and a known risk factor for impaired development in very-low-birth-weight (VLBW) neonates. We report a population of 288 patients with no or with low-grade MRI lesions scanned at a term equivalent age (TEA) born between 2012 and 2018. Griffiths Mental Development Scale II (GMDS II) at 2 and 3 years, preterm complications and weight growth were retrospectively analyzed. EUGR was defined for weight z-score ˂ 10 percentile at TEA, 6 and 12 months of correct age or as z-score decreased by 1-point standard deviation (SDS) from birth to TEA and from TEA to 6 months. Multivariate analysis showed that a higher weight z-score at 6 months is protective for the global developmental quotient (DQ) at 2 years (OR 0.74; CI 95% 0.59–0.93; p = 0.01). EUGR at 6 months was associated with worse locomotor, personal/social, language and performance DQ at 2 years and worse language and practical reasoning DQ at 3 years. In conclusion, a worse weight z-score at 6 months of age seems to be an independent risk factor for significantly reduced GMDS in many areas. These results suggest that we should invest more into post-discharge nutrition, optimizing family nutritional education.
Assessment of Individual and Mixed Effects of Six Minerals on Thyroid Hormones in Chinese Pregnant Women
Authors: Gu Simeng , Mo Zhe , Chen Zhijian , Li Xueqing , Jiang Yujie , Liu Chenyang , Guo Fanjia , Li Yahui , Mao Guangming , Huang Xuemin , Wang Xiaofeng
Published: February 2024

The biosynthesis of thyroid hormones is essential for brain and neurological development. It requires iodine as a key component but is also influenced by other nutrients. Evidence for the combined nutrient status in relation to thyroid hormones during pregnancy is limited. We aimed to investigate the joint associations of iodine, selenium, zinc, calcium, magnesium and iron with maternal thyroid functions in 489 pregnant women from Hangzhou, China. Serum levels of six essential minerals and thyroid function parameters were measured during the first antenatal visit. Linear regression, quantile g-computation and Bayesian kernel machine regression were used to explore the individual and joint relationships between the six minerals and thyroid hormones. Linear regression analyses revealed that calcium was positively associated with free triiodothyronine (FT3). Zinc was positively associated with free thyroxine (FT4). Iodine was negatively associated with thyroid-stimulating hormone (TSH) and positively associated with FT3 and FT4. The quantile g-computation and BKMR models indicated that the joint nutrient concentration was negatively associated with TSH and positively associated with FT3 and FT4. Among the six minerals, iodine contributed most to thyroid function. The findings suggested that maintaining the appropriate concentration of minerals, either as individuals or a mixture, is important for thyroid health during pregnancy.
Erigeron annuus Extract Improves DNCB-Induced Atopic Dermatitis in a Mouse Model via the Nrf2/HO-1 Pathway
Authors: Jeong Myeongguk , Kwon Hyeokjin , Kim Yeeun , Jin Hyunwoo , Choi Go-Eun , Hyun Kyung-Yae
Published: February 2024

Atopic dermatitis (AD) is a persistent inflammatory skin condition resulting from an intricate interplay among genetic, immunological, and environmental factors. Erigeron annuus (EA), an annual winter plant belonging to the family Asteraceae, possesses anti-inflammatory, cytoprotective, and antioxidant activities. In this study, we hypothesized that Erigeron annuus extract (EAE) could be an effective agent for ameliorating AD-like symptoms. To confirm this hypothesis in vitro, we used H2O2-stimulated human keratinocytes (HaCaT cells) to demonstrate that pre-treatment with EAE protected against oxidative stress. HaCaT cells pretreated with EAE and stimulated with H2O2 showed decreased intracellular malondialdehyde content, increased superoxide dismutase activity, and reduced intracellular reactive oxygen species accumulation. To verify the in vivo hypothesis based on the intracellular results, an AD disease mouse model was induced with 1-chloro-2,4-dinitrobenzene (DNCB), and EAE was orally administered at a non-toxic concentration according to the toxicity evaluation results. The results showed that AD disease models in BALB/c mice exhibited reduced ear epidermal thickness, scratching behavior, and mast cell infiltration. In conclusion, our results indicate that EAE has the potential to improve AD by upregulating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway.
Ready-to-Use Multichamber Bags in Home Parenteral Nutrition for Patients with Advanced Cancer: A Single-Center Prospective Study
Authors: Fernández-Argüeso María , Gómez-Bayona Elena , Ugalde Beatriz , Vega-Piñero Belén , Gil-Díaz Mayra , Longo Federico , Pintor Rosario , Botella-Carretero José I.
Published: February 2024

Home parenteral nutrition (HPN) is increasingly prescribed for patients with advanced cancer. This therapy improves free-fat mass, quality of life and survival, but it is not free from complications, especially catheter-related bloodstream infections (CRBSIs). The use of commercial multichamber bags in HPN has not been extensively explored in oncologic patients and their association with complications is not well known. In this prospective cohort study, we included 130 patients with advanced cancer and HPN. We compared the effects of individual compounded bags (n = 87) vs. commercial multichamber bags (n = 43) on complications. There were no differences in any complication, including thrombosis (p > 0.05). There were 0.28 episodes of CRBSI per 1000 catheter days in the individual compounded bag group and 0.21 in the multichamber bag group (p > 0.05). A total of 34 patients were weaned off HPN, 22 with individual bags and 12 with multichamber bags (p = 0.749). Regarding survival when on HPN, the group with individual bags showed a median of 98 days (95% CI of 49–147), whereas those with multichamber bags showed a median of 88 days (95% CI of 43–133 (p = 0.913)). In conclusion, commercial multichamber bags for HPN in patients with advanced cancer are non-inferior when compared to individual compounded bags in terms of complications.
The Effects of Yeast Protein on Gut Microbiota in Mice When Compared with Soybean Protein and Whey Protein Isolates
Authors: Zhou Xuewei , Liang Li , Sun Baoguo , Li Ku , Guo Hui , Zhang Yuyu
Published: February 2024

Different protein sources can impact gut microbiota composition and abundance, and also participate in health regulation. In this study, mice were gavaged with yeast protein (YP), soybean protein isolate (SPI), and whey protein isolate (WPI) for 28 days. Body weights showed similar patterns across different protein administration groups. The ileum in YP-supplemented mice exhibited good morphology, and tight-junction (TJ) proteins were slightly upregulated. Immunoglobulin (Ig)A, IgM, and IgG levels in the ileum of different protein groups were significantly increased (p < 0.05). Interleukin (IL)-10 levels were significantly increased, whereas IL-6 levels were significantly reduced in the YP group when compared with the control (C) (p < 0.05). Glutathione peroxidase (GSH-Px) levels in the ileum were significantly increased in the YP group (p < 0.05). These results indicate that YP potentially improved intestinal immunity and inflammatory profiles. The relative abundances of Parabacteroides, Prevotella, and Pseudobutyrivibrio in the YP group were more enriched when compared with the C and SPI groups, and Parabacteroides was significantly upregulated when compared with the WPI group (p < 0.05). Overall, the results indicate that YP upregulates the beneficial bacteria and improves ileal immunity and anti-inflammatory capabilities.
Carbohydrate Oral Rinsing, Cycling Performance and Individual Complex Carbohydrate Taste Sensitivity
Authors: Hartley Claudia , Carr Amelia , Roberts Spencer S. H. , Bredie Wender L. P. , Keast Russell S. J.
Published: February 2024

The aim of this pilot study was to determine the effect of individual complex carbohydrate taste sensitivity on cycling performance with complex carbohydrate oral rinsing. Ten male participants completed five cycling time trials in a fasted state with a seven-day washout period between each trial. Participants completed a fixed amount of work (738.45 ± 150.74 kJ) as fast as possible on a cycle ergometer while rinsing with an oral rinse for 10 s every 12.5% of the trial. An oral rinse (maltodextrin, oligofructose, glucose, sucralose or water control) was given per visit in a randomised, crossover, blinded design. Afterwards, participants had their taste assessed with three stimuli, complex carbohydrate (maltodextrin), sweet (glucose) and sour (citric acid), using taste assessment protocol to determine individual taste sensitivity status. Participants were subsequently grouped according to their complex carbohydrate taste sensitivity and complex carbohydrate taste intensity. There were no significant effects of the oral rinses on cycling performance time (p = 0.173). Participants who did not have improvements in exercise performance with the maltodextrin rinse experienced a stronger taste intensity with complex carbohydrate stimuli at baseline (p = 0.047) and overall (p = 0.047) than those who did have improvements in performance. Overall, a carbohydrate oral rinse was ineffective in significantly improving cycling performance in comparison with a water control. However, when participants were grouped according to complex carbohydrate taste intensity, differences in exercise performance suggest that individual sensitivity status to complex carbohydrates could impact the efficacy of a carbohydrate-based oral rinse.
Why Is the Duration of Erythema Migrans at Diagnosis Longer in Patients with Lyme Neuroborreliosis Than in Those without Neurologic Involvement?
Authors: Ogrinc Katarina , Bogovič Petra , Maraspin Vera , Lotrič-Furlan Stanka , Rojko Tereza , Kastrin Andrej , Strle Klemen , Wormser Gary P. , Strle Franc
Published: February 2024

In prior studies, the skin lesion erythema migrans (EM) was present for a longer time period before diagnosis of concomitant borrelial meningoradiculoneuritis (Bannwarth’s syndrome) compared to EM patients without neurologic symptoms. To determine if this observation pertains to other manifestations of Lyme neuroborreliosis (LNB), we compared EM characteristics in patients with borrelial meningoradiculoneuritis (n = 122) to those with aseptic meningitis without radicular pain (n = 72 patients), and to patients with EM but without neurologic involvement (n = 12,384). We also assessed factors that might impact duration. We found that the duration of EM at diagnosis in patients with borrelial meningoradiculoneuritis was not significantly different compared with those with LNB without radicular pain (34 vs. 26 days; p = 0.227). The duration of EM for each of these clinical presentations of LNB, however, was significantly longer than in patients with EM without LNB (10 days; p < 0.001). Contributing factors to this difference might have been that patients with LNB failed to recognize that they had EM or were unaware of the importance of not delaying antibiotic treatment for EM. In conclusion, the duration of the EM skin lesion in EM patients with LNB is longer than in patients with just EM, irrespective of the type of LNB.
Understanding Smallholder Pigkeepers’ Awareness and Perceptions of African Swine Fever and Its Control Measures in Ukraine
Authors: Moskalenko Lidiia , Schulz Katja , Nedosekov Vitalii , Mõtus Kerli , Viltrop Arvo
Published: February 2024

African swine fever (ASF) has posed a significant threat to Ukrainian pig farming since its identification in 2012. In this study, recognising the pivotal role of pigkeepers in disease control, we conducted ten focus groups involving 52 smallholders across eight regions in Ukraine. Using participatory methods, we revealed their awareness of ASF signs, transmission routes, preventive measures, and the perceptions of stakeholders involved in ASF control. Furthermore, we identified the smallholders’ acceptance of eradication and restriction measures, the perceived impact of zoning consequences, and their main sources of ASF information. Smallholders identified fever and skin haemorrhage as the most indicative signs of ASF and highlighted rodents as a primary transmission concern. Disinfection was seen as the most effective measure for preventing the introduction of ASF. Pigkeepers who perceived their stakeholder role in ASF control showed more trust in themselves and veterinarians than in central veterinary authorities. Farm-level ASF eradication measures were generally accepted; however, culling within the protection zone was least accepted, with economic losses listed as the most impactful consequence for pigkeepers. For ASF information, pigkeepers favour web searches and veterinarians, as well as traditional media and word-of-mouth news. This study provides valuable insights into refining the ASF communication strategies in Ukraine.
A Bird’s-Eye View of the Pathophysiologic Role of the Human Urobiota in Health and Disease: Can We Modulate It?
Authors: Jirillo Emilio , Palmirotta Raffaele , Colella Marica , Santacroce Luigi
Published: February 2024

For a long time, urine has been considered sterile in physiological conditions, thanks to the particular structure of the urinary tract and the production of uromodulin or Tamm–Horsfall protein (THP) by it. More recently, thanks to the development and use of new technologies, i.e., next-generation sequencing and expanded urine culture, the identification of a microbial community in the urine, the so-called urobiota, became possible. Major phyla detected in the urine are represented by Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. Particularly, the female urobiota is largely represented by Lactobacillus spp., which are very active against urinary pathogenic Escherichia (E.) coli (UPEC) strains via the generation of lactic acid and hydrogen peroxide. Gut dysbiosis accounts for recurrent urinary tract infections (UTIs), so-called gut–bladder axis syndrome with the formation of intracellular bacterial communities in the course of acute cystitis. However, other chronic urinary tract infections are caused by bacterial strains of intestinal derivation. Monomicrobial and polymicrobial infections account for the outcome of acute and chronic UTIs, even including prostatitis and chronic pelvic pain. E. coli isolates have been shown to be more invasive and resistant to antibiotics. Probiotics, fecal microbial transplantation, phage therapy, antimicrobial peptides, and immune-mediated therapies, even including vaccines for the treatment of UTIs, will be described.
Targeting Hypoxia-Inducible Factor-1 (HIF-1) in Cancer: Emerging Therapeutic Strategies and Pathway Regulation
Authors: Qannita Reem A. , Alalami Ayah I. , Harb Amani A. , Aleidi Shereen M. , Taneera Jalal , Abu-Gharbieh Eman , El-Huneidi Waseem , Saleh Mohamed A. , Alzoubi Karem H. , Semreen Mohammad H. , Hudaib Mohammad , Bustanji Yasser
Published: February 2024

Hypoxia-inducible factor-1 (HIF-1) is a key regulator for balancing oxygen in the cells. It is a transcription factor that regulates the expression of target genes involved in oxygen homeostasis in response to hypoxia. Recently, research has demonstrated the multiple roles of HIF-1 in the pathophysiology of various diseases, including cancer. It is a crucial mediator of the hypoxic response and regulator of oxygen metabolism, thus contributing to tumor development and progression. Studies showed that the expression of the HIF-1α subunit is significantly upregulated in cancer cells and promotes tumor survival by multiple mechanisms. In addition, HIF-1 has potential contributing roles in cancer progression, including cell division, survival, proliferation, angiogenesis, and metastasis. Moreover, HIF-1 has a role in regulating cellular metabolic pathways, particularly the anaerobic metabolism of glucose. Given its significant and potential roles in cancer development and progression, it has been an intriguing therapeutic target for cancer research. Several compounds targeting HIF-1-associated processes are now being used to treat different types of cancer. This review outlines emerging therapeutic strategies that target HIF-1 as well as the relevance and regulation of the HIF-1 pathways in cancer. Moreover, it addresses the employment of nanotechnology in developing these promising strategies.
Targeted Covalent Inhibitors in Drug Discovery, Chemical Biology and Beyond
Authors: Serafim Ricardo A. M. , Gehringer Matthias , Borsari Chiara
Published: February 2024

Covalent inhibitors have experienced a revival in medicinal chemistry and chemical biology in recent decades [...]
Versatile Peptide-Based Nanosystems for Photodynamic Therapy
Authors: Li Qiuyan , Ming Ruiqi , Huang Lili , Zhang Ruoyu
Published: February 2024

Photodynamic therapy (PDT) has become an important therapeutic strategy because it is highly controllable, effective, and does not cause drug resistance. Moreover, precise delivery of photosensitizers to tumor lesions can greatly reduce the amount of drug administered and optimize therapeutic outcomes. As alternatives to protein antibodies, peptides have been applied as useful targeting ligands for targeted biomedical imaging, drug delivery and PDT. In addition, other functionalities of peptides such as stimuli responsiveness, self-assembly, and therapeutic activity can be integrated with photosensitizers to yield versatile peptide-based nanosystems for PDT. In this article, we start with a brief introduction to PDT and peptide-based nanosystems, followed by more detailed descriptions about the structure, property, and architecture of peptides as background information. Finally, the most recent advances in peptide-based nanosystems for PDT are emphasized and summarized according to the functionalities of peptide in the system to reveal the design and development principle in different therapeutic circumstances. We hope this review could provide useful insights and valuable reference for the development of peptide-based nanosystems for PDT.
Liposome Encapsulation of the Palmitoyl–KTTKS Peptide: Structural and Functional Characterization
Authors: Vitali Alberto , Paolicelli Patrizia , Bigi Barbara , Trilli Jordan , Di Muzio Laura , Carriero Vito Cosimo , Casadei Maria Antonietta , Petralito Stefania
Published: February 2024

In this study, the amphiphilic N-palmitoyl–KTTKS peptide was integrated in the bilayer of egg-derived phosphatidylcholine (PC) vesicles using two different preparation methods, namely thin-film evaporation (TLE) and reverse-phase evaporation (REV). Both the REV and TLE methods allowed for the formation of homogeneous liposome dispersions (PdI < 0.20) with mean hydrodynamic diameters of <100 nm and <200 nm, respectively, a net negative surface charge and a percentage of structured phospholipids higher than 90%. The inclusion of the amphiphilic N-palmitoyl–KTTKS peptide within phospholipid-based vesicles could improve peptide stability and skin delivery. Therefore, the obtained liposomes were evaluated via experiments assessing the synthesis of collagen and the ECM in 3T3-NIH fibroblasts. The obtained results showed that, when delivered with PC liposomes, pal-KTTKS stimulated collagen production more than free pentapeptide and 1 mM ascorbic acid, used as a positive control.
Development of Ciprofloxacin-Loaded Electrospun Yarns of Application Interest as Antimicrobial Surgical Suture Materials
Authors: Teno Jorge , Pardo-Figuerez Maria , Evtoski Zoran , Prieto Cristina , Cabedo Luis , Lagaron Jose M.
Published: February 2024

Surgical site infections (SSI) occur very frequently during post-operative procedures and are often treated with oral antibiotics, which may cause some side effects. This type of infection could be avoided by encapsulating antimicrobial/anti-inflammatory drugs within the surgical suture materials so that they can more efficiently act on the site of action during wound closure, avoiding post-operative bacterial infection and spreading. This work was aimed at developing novel electrospun bio-based anti-infective fibre-based yarns as novel suture materials for preventing surgical site infections. For this, yarns based on flying intertwined microfibres (1.95 ± 0.22 µm) were fabricated in situ during the electrospinning process using a specially designed yarn collector. The electrospun yarn sutures (diameter 300–500 µm) were made of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with different contents of 3HV units and contained ciprofloxacin hydrochloride (CPX) as the antimicrobial active pharmaceutical ingredient (API). The yarns were then analysed by scanning electron microscopy, Fourier transform infrared spectroscopy, wide-angle X-ray scattering, differential scanning calorimetry, and in vitro drug release. The yarns were also analysed in terms of antimicrobial and mechanical properties. The material characterization indicated that the varying polymer molecular architecture affected the attained polymer crystallinity, which was correlated with the different drug-eluting profiles. Moreover, the materials exhibited the inherent stiff behaviour of PHBV, which was further enhanced by the API. Lastly, all the yarn sutures presented antimicrobial properties for a time release of 5 days against both Gram-positive and Gram-negative pathogenic bacteria. The results highlight the potential of the developed antimicrobial electrospun yarns in this study as potential innovative suture materials to prevent surgical infections.
Nanoparticles of Thiolated Xanthan Gum for the Oral Delivery of Miconazole Nitrate: In Vitro and In Vivo Evaluation
Authors: Namazi Nader I. , Alrbyawi Hamad , Alanezi Abdulkareem Ali , Almuqati Afaf F , Shams Anwar , Ali Hany S. M.
Published: February 2024

The objective of this research was to develop a mucoadhesive delivery system that improves permeation for the administration of poorly absorbed oral medications. Thiolation of xanthan gum (XGM) was carried out by esterification with mercaptobutyric acid. Fourier-transformed infrared spectroscopy was used to confirm thiol-derivatization. Using Ellman’s technique, it was revealed that the xanthan-mercaptobutyric acid conjugate had 4.7 mM of thiol groups in 2 mg/mL of polymeric solution. Using mucosa of sheep intestine, the mucoadhesive properties of XGM and thiolated xanthan gum (TXGM) nanoparticles were investigated and we found that TXGM had a longer bioadhesion time than XGM. The disulfide link that forms between mucus and thiolated XGM explains why it has better mucoadhesive properties than XGM. A study on in vitro miconazole (MCZ) release using phosphate buffer (pH 6.8) found that TXGM nanoparticles released MCZ more steadily than MCZ dispersion did. A 1-fold increase in the permeation of MCZ was observed from nanoparticles using albino rat intestine compared to MCZ. Albino rats were used to test the pharmacokinetics of MCZ, and the results showed a 4.5-fold increase in bioavailability. In conclusion, the thiolation of XGM enhances its bioavailability, controlled release of MCZ for a long period of time, and mucoadhesive activity.
Thermosensitive Polymeric Nanoparticles for Drug Co-Encapsulation and Breast Cancer Treatment
Authors: Dartora Vanessa Franco Carvalho , Passos Julia S. , Costa-Lotufo Leticia V. , Lopes Luciana B. , Panitch Alyssa
Published: February 2024

Despite advances in breast cancer treatment, there remains a need for local management of noninvasive, low-grade ductal carcinoma in situ (DCIS). These focal lesions are well suited for local intraductal treatment. Intraductal administration supported target site drug retention, improved efficacy, and reduced systemic exposure. Here, we used a poly(N-isopropyl acrylamide, pNIPAM) nanoparticle delivery system loaded with cytotoxic piplartine and an MAPKAP Kinase 2 inhibitor (YARA) for this purpose. For tumor environment targeting, a collagen-binding peptide SILY (RRANAALKAGELYKSILYGSG-hydrazide) was attached to pNIPAM nanoparticles, and the nanoparticle diameter, zeta potential, drug loading, and release were assessed. The system was evaluated for cytotoxicity in a 2D cell culture and 3D spheroids. In vivo efficacy was evaluated using a chemical carcinogenesis model in female Sprague–Dawley rats. Nanoparticle delivery significantly reduced the IC50 of piplartine (4.9 times) compared to the drug in solution. The combination of piplartine and YARA in nanoparticles further reduced the piplartine IC50 (~15 times). Treatment with these nanoparticles decreased the in vivo tumor incidence (5.2 times). Notably, the concentration of piplartine in mammary glands treated with nanoparticles (35.3 ± 22.4 μg/mL) was substantially higher than in plasma (0.7 ± 0.05 μg/mL), demonstrating targeted drug retention. These results indicate that our nanocarrier system effectively reduced tumor development with low systemic exposure.
Comparison between Different Optical Configurations of Active-FRAME Setup in Multispectral Imaging of Flames
Authors: Chorey Devashish , Boggavarapu Prasad , Deshmukh Devendra , Rayavarapu Ravikrishna , Mishra Yogeshwar Nath
Published: February 2024

Snapshot multispectral imaging of chemical species in the flame is essential for improved understanding of the combustion process. In this article, we investigate the different configurations of a structured laser sheet-based multispectral imaging approach called the Frequency Recognition Algorithm for Multiple Exposures (FRAME). Using FRAME, a snapshot of Laser-Induced Fluorescence (LIF) of Polycyclic Aromatic Hydrocarbons (PAH) excited by 283.5 nm laser and Laser-Induced Incandescence (LII) of soot particles excited by 532 nm laser are acquired simultaneously on a single FRAME image. A laminar diffusion flame of acetylene produced by a Gülder burner is used for the experiments. The standard FRAME approach is based on creating two spatially modulated laser sheets and arranging them in a cross-patterned configuration (X). However, the effect of using different configurations (angles) of the two laser sheets on the multispectral planar imaging of the flame has not yet been studied. Therefore, we have compared the FRAME approach in four different configurations while keeping the same flame conditions. First, we have compared the relation between laser fluence and LII signals with and without spatial modulation of the 532 nm laser sheet and found that both detections follow the same curve. When comparing the maps of flame species reconstructed from the standard FRAME configuration and other configurations, there are some dissimilarities. These differences are attributed to minor changes in the imaging plane, optical alignment, laser path length, different modulation frequencies of the laser sheet, laser extinction, laser fluence, etc.
CO2 Measurement under Different Pressure and Vibration Conditions Using Tunable Diode Laser Absorption Spectroscopy
Authors: Ban Deyue , Li Nan , Zheng Yongqiu , Xue Chenyang
Published: February 2024

The greenhouse effect resulting from fuel combustion has drawn growing attention, and CO2 emissions from fossil fuel power stations are one of the main sources of greenhouse gases. It is crucial to monitor the concentration of CO2 in the flue gas ducts of these stations. However, pressure and vibration caused by the combustion of boilers make the measurement of CO2 in flue gas ducts extremely challenging. In this study, tunable diode laser absorption spectroscopy (TDLAS) combined with Wave Modulation Spectroscopy (WMS) was employed to measure the concentration of CO2 under different pressure and vibration conditions in the laboratory. The absorption line of CO2 at the wavenumber 6357.38 cm−1 was recorded under varying pressure conditions ranging from 0 to 1.4 atm, acceleration conditions ranging from 0 to 7.7 m/s2, and a combination of both. Firstly, a negative linear correlation was found between the pressure and the amplitude of the second harmonic, with a maximum relative error of 4.645% observed at a pressure of 1.4 atm. Secondly, the maximum acceleration that the system can withstand was determined to be 7.3 m/s2, as it was not possible to provide a sufficiently low fitting error at higher accelerations. For the combined effects of the pressure and vibration, a dramatic increase in the relative error of amplitude can be observed within the acceleration range of 5.0~6.0 m/s2 while under the pressure conditions of 0.6 atm, 1.0 atm, and 1.4 atm. Moreover, the maximum endurable acceleration decreases with the increase in pressure, which infers that effective measurements can be achieved when the acceleration is below 5 m/s2 within the pressure range of 0~1.4 atm. This suggests that TDLAS combined with WMS possesses a potential for online measuring of CO2 concentrations in flue gas ducts within a certain acceleration range. This work can provide some insights for stable gas detection using TDLAS under varied pressure and vibration conditions.
Beneficial Effect of Exogenously Applied Calcium Pyruvate in Alleviating Water Deficit in Sugarcane as Assessed by Chlorophyll a Fluorescence Technique
Authors: Dias Mirandy dos Santos , da Silva Francisco de Assis , Fernandes Pedro Dantas , Farias Carlos Henrique de Azevedo , de Lima Robson Felipe , da Silva Maria de Fátima Caetano , Lima Vitória Régia do Nascimento , de Lima Andrezza Maia , de Lacerda Cassiano Nogueira , Reis Lígia Sampaio , Souza Weslley Bruno Belo de , Silva André Alisson Rodrigues da , Arruda Thiago Filipe de Lima
Published: February 2024

The growing demand for food production has led to an increase in agricultural areas, including many with low and irregular rainfall, stressing the importance of studies aimed at mitigating the harmful effects of water stress. From this perspective, the objective of this study was to evaluate calcium pyruvate as an attenuator of water deficit on chlorophyll a fluorescence of five sugarcane genotypes. The experiment was conducted in a plant nursery where three management strategies (E1—full irrigation, E2—water deficit with the application of 30 mM calcium pyruvate, and E3—water deficit without the application of calcium pyruvate) and five sugarcane genotypes (RB863129, RB92579, RB962962, RB021754, and RB041443) were tested, distributed in randomized blocks, in a 3 × 5 factorial design with three replications. There is dissimilarity in the fluorescence parameters and photosynthetic pigments of the RB863129 genotype in relation to those of the RB041443, RB96262, RB021754, and RB92579 genotypes. Foliar application of calcium pyruvate alleviates the effects of water deficit on the fluorescence parameters of chlorophyll a and photosynthetic pigments in sugarcane, without interaction with the genotypes. However, subsequent validation tests will be necessary to test and validate the adoption of this technology under field conditions.
Utilizing Two Populations Derived from Tropical Maize for Genome-Wide Association Analysis of Banded Leaf and Sheath Blight Resistance
Authors: Li Shaoxiong , Jiang Fuyan , Bi Yaqi , Yin Xingfu , Li Linzhuo , Zhang Xingjie , Li Jinfeng , Liu Meichen , Shaw Ranjan K. , Fan Xingming
Published: February 2024

Banded leaf and sheath blight (BLSB) in maize is a soil-borne fungal disease caused by Rhizoctonia solani Kühn, resulting in significant yield losses. Investigating the genes responsible for regulating resistance to BLSB is crucial for yield enhancement. In this study, a multiparent maize population was developed, comprising two recombinant inbred line (RIL) populations totaling 442 F8RILs. The populations were generated by crossing two tropical inbred lines, CML444 and NK40-1, known for their BLSB resistance, as female parents, with the high-yielding but BLSB-susceptible inbred line Ye107 serving as the common male parent. Subsequently, we utilized 562,212 high-quality single nucleotide polymorphisms (SNPs) generated through genotyping-by-sequencing (GBS) for a comprehensive genome-wide association study (GWAS) aimed at identifying genes responsible for BLSB resistance. The objectives of this study were to (1) identify SNPs associated with BLSB resistance through genome-wide association analyses, (2) explore candidate genes regulating BLSB resistance in maize, and (3) investigate pathways involved in BLSB resistance and discover key candidate genes through Gene Ontology (GO) analysis. The GWAS analysis revealed nineteen SNPs significantly associated with BLSB that were consistently identified across four environments in the GWAS, with phenotypic variation explained (PVE) ranging from 2.48% to 11.71%. Screening a 40 kb region upstream and downstream of the significant SNPs revealed several potential candidate genes. By integrating information from maize GDB and the NCBI, we identified five novel candidate genes, namely, Zm00001d009723, Zm00001d009975, Zm00001d009566, Zm00001d009567, located on chromosome 8, and Zm00001d026376, on chromosome 10, related to BLSB resistance. These candidate genes exhibit association with various aspects, including maize cell membrane proteins and cell immune proteins, as well as connections to cell metabolism, transport, transcriptional regulation, and structural proteins. These proteins and biochemical processes play crucial roles in maize defense against BLSB. When Rhizoctonia solani invades maize plants, it induces the expression of genes encoding specific proteins and regulates corresponding metabolic pathways to thwart the invasion of this fungus. The present study significantly contributes to our understanding of the genetic basis of BLSB resistance in maize, offering valuable insights into novel candidate genes that could be instrumental in future breeding efforts to develop maize varieties with enhanced BLSB resistance.
Nanostructured Poly-l-lactide and Polyglycerol Adipate Carriers for the Encapsulation of Usnic Acid: A Promising Approach for Hepatoprotection
Authors: Brugnoli Benedetta , Perna Greta , Alfano Sara , Piozzi Antonella , Galantini Luciano , Axioti Eleni , Taresco Vincenzo , Mariano Alessia , Scotto d’Abusco Anna , Vecchio Ciprioti Stefano , Francolini Iolanda
Published: February 2024

The present study investigates the utilization of nanoparticles based on poly-l-lactide (PLLA) and polyglycerol adipate (PGA), alone and blended, for the encapsulation of usnic acid (UA), a potent natural compound with various therapeutic properties including antimicrobial and anticancer activities. The development of these carriers offers an innovative approach to overcome the challenges associated with usnic acid’s limited aqueous solubility, bioavailability, and hepatotoxicity. The nanosystems were characterized according to their physicochemical properties (among others, size, zeta potential, thermal properties), apparent aqueous solubility, and in vitro cytotoxicity. Interestingly, the nanocarrier obtained with the PLLA-PGA 50/50 weight ratio blend showed both the lowest size and the highest UA apparent solubility as well as the ability to decrease UA cytotoxicity towards human hepatocytes (HepG2 cells). This research opens new avenues for the effective utilization of these highly degradable and biocompatible PLLA-PGA blends as nanocarriers for reducing the cytotoxicity of usnic acid.
Preparation of PLGA Microspheres Using the Non-Toxic Glycofurol as Polymer Solvent by a Modified Phase Inversion Methodology
Authors: Sobel Douglas , Ramasubramanian Barath , Sawhney Puja , Parmar Keerat
Published: February 2024

Poly(D,L-lactide-co-glycolide is a biodegradable copolymer that can release pharmaceuticals. These pharmaceuticals can provide local therapy and also avert the clinical issues that occur when a drug must be given continuously and/or automatically. However, the drawbacks of using poly(D,L-lactide-co-glycolide include the kinetics and duration of time of poly(D,L-lactide-co-glycolide drug release, the denaturing of the drug loaded drug, and the potential clinical side effects. These drawbacks are mainly caused by the volatile organic solvents needed to prepare poly(D,L-lactide-co-glycolide spheres. Using the non-toxic solvent glycofurol solvent instead of volatile organic solvents to construct poly(D,L-lactide-co-glycolide microspheres may deter the issues of using volatile organic solvents. Up to now, preparation of such glycofurol spheres has previously met with limited success. We constructed dexamethasone laden poly(D,L-lactide-co-glycolide microspheres utilizing glycofurol as the solvent within a modified phase inversion methodology. These prepared microspheres have a higher drug load and a lower rate of water diffusion. This prolongs drug release compared to dichloromethane constructed spheres. The glycofurol-generated spheres are also not toxic to target cells as is the case for dichloromethane-constructed spheres. Further, glycofurol-constructed spheres do not denature the dexamethasone molecule and have kinetics of drug release that are more clinically advantageous, including a lower drug burst and a prolonged drug release.
Machine Learning Backpropagation Prediction and Analysis of the Thermal Degradation of Poly (Vinyl Alcohol)
Authors: Otaru Abdulrazak Jinadu , Alhulaybi Zaid Abdulhamid , Dubdub Ibrahim
Published: February 2024

Thermogravimetric analysis (TGA) is crucial for describing polymer materials’ thermal behavior as a result of temperature changes. While available TGA data substantiated in the literature significantly focus attention on TGA performed at higher heating rates, this study focuses on the machine learning backpropagation analysis of the thermal degradation of poly (vinyl alcohol), or PVA, at low heating rates, typically 2, 5 and 10 K/min, at temperatures between 25 and 600 °C. Initial TGA analysis showed that a consistent increase in heating rate resulted in an increase in degradation temperature as the resulting thermograms shifted toward a temperature maxima. At degradation temperatures between 205 and 405 °C, significant depths in the characterization of weight losses were reached, which may be attributed to the decomposition and loss of material content. Artificial neural network backpropagation of machine learning algorithms were used for developing mathematical descriptions of the percentage weight loss (output) by these PVA materials as a function of the heating rate (input 1) and degradation temperature (input 2) used in TGA analysis. For all low heating rates, modelling predictions were observably correlated with experiments with a 99.2% correlation coefficient and were used to interpolate TGA data at 3.5 and 7.5 K/min, indicating trends strongly supported by experimental TGA data as well as literature research. Thus, this approach could provide a useful tool for predicting the thermograms of PVA materials at low heating rates and contribute to the development of more advanced PVA/polymer materials for home and industrial applications.
Catalysis of Silver and Bismuth in Various Epoxy Resins
Authors: Jeong Hayun , Jang Keon-Soo
Published: February 2024

Epoxy resins find extensive utility across diverse applications owing to their exceptional adhesion capabilities and robust mechanical and thermal characteristics. However, the demanding reaction conditions, including extended reaction times and elevated reaction temperature requirements, pose significant challenges when using epoxy resins, particularly in advanced applications seeking superior material properties. To surmount these limitations, the conventional approach involves incorporating organic catalysts. Within the ambit of this investigation, we explored the catalytic potential of metallic powders, specifically bismuth (Bi) and silver (Ag), in epoxy resins laden with various curing agents, such as diacids, anhydrides, and amines. Metallic powders exhibited efficacious catalytic activity in epoxy–diacid and epoxy–anhydride systems. In contrast, their influence on epoxy–amine systems was rendered negligible, attributed to the absence of requisite carboxylate functional groups. Additionally, the catalytic performance of Bi and Ag are different, with Bi displaying superior efficiency owing to the presence of inherent metal oxide layers on its powder surfaces. Remarkably, the thermal and mechanical properties of uncatalyzed, fully cured epoxy resins closely paralleled those of their catalyzed counterparts. These findings accentuate the potential of Bi and Ag metal catalysts, particularly in epoxy–diacid and epoxy–anhydride systems, spanning a spectrum of epoxy-based applications. In summary, this investigation elucidates the catalytic capabilities of Bi and Ag metal powders, underscoring their ability to enhance the curing rate of epoxy resin systems involving diacids and anhydrides but not amines. This research points toward a promising trajectory for multifarious epoxy-related applications.
Wastewater Treatment Using Membrane Bioreactor Technologies: Removal of Phenolic Contaminants from Oil and Coal Refineries and Pharmaceutical Industries
Authors: Khan Mohd , Wibowo Agung , Karim Zoheb , Posoknistakul Pattaraporn , Matsagar Babasaheb , Wu Kevin , Sakdaronnarong Chularat
Published: February 2024

Huge amounts of noxious chemicals from coal and petrochemical refineries and pharmaceutical industries are released into water bodies. These chemicals are highly toxic and cause adverse effects on both aquatic and terrestrial life. The removal of hazardous contaminants from industrial effluents is expensive and environmentally driven. The majority of the technologies applied nowadays for the removal of phenols and other contaminants are based on physio-chemical processes such as solvent extraction, chemical precipitation, and adsorption. The removal efficiency of toxic chemicals, especially phenols, is low with these technologies when the concentrations are very low. Furthermore, the major drawbacks of these technologies are the high operation costs and inadequate selectivity. To overcome these limitations, researchers are applying biological and membrane technologies together, which are gaining more attention because of their ease of use, high selectivity, and effectiveness. In the present review, the microbial degradation of phenolics in combination with intensified membrane bioreactors (MBRs) has been discussed. Important factors, including the origin and mode of phenols’ biodegradation as well as the characteristics of the membrane bioreactors for the optimal removal of phenolic contaminants from industrial effluents are considered. The modifications of MBRs for the removal of phenols from various wastewater sources have also been addressed in this review article. The economic analysis on the cost and benefits of MBR technology compared with conventional wastewater treatments is discussed extensively.
The Influence of the Flexibility of a Polymeric Adhesive Layer on the Mechanical Response of a Composite Reinforced Concrete Slab and a Reinforced Concrete Beam Girder
Authors: Szeptyński Paweł , Pochopień Jan Grzegorz , Jasińska Dorota , Kwiecień Arkadiusz
Published: February 2024

This study addresses the challenges of modeling flexible connections in composite structures employing a polymeric adhesive layer. These types of connections provide a more uniform stress distribution compared to conventional rigid connectors. However, they lack standardized design rules and still require much research to sufficiently comprehend their properties. The novelty of this research lies in proposing an analytical solution to address these issues. Its aim is to investigate the influence of the stiffness of the polymer adhesive on the girder’s deflection and on the maximum stresses in both the adhesive and concrete. The analyzed composite structure consists of a reinforced concrete (RC) slab and an RC beam connected with a layer of flexible polyurethane (FPU) adhesive. Analytical and numerical approaches for the description of the mechanical response of a composite bridge girder are presented. Another objective is to validate the analytical design formulas using 3D nonlinear numerical analysis, both in the case of uncracked and cracked concrete. Seven types of FPUs are tested in the uniaxial tension test, each examined at five strain rates. The obtained data is used to predict the mechanical response of the considered girder using finite element analysis (FEA) as well as with a simplified one-dimensional composite beam theory. Fair agreement is found between the FEA results and theoretical predictions. A comparison of the results obtained for these two models is performed, and the similarities and discrepancies are highlighted and discussed.
Precise Lightning Strike Detection in Overhead Lines Using KL-VMD and PE-SGMD Innovations
Authors: Dong Xinsheng , Liu Jucheng , He Shan , Han Lu , Dong Zhongkai , Cai Minbo
Published: February 2024

When overhead lines are impacted by lightning, the traveling wave of the fault contains a wealth of fault information. The accurate extraction of feature quantities from transient components and their classification are fundamental to the identification of lightning faults. The extraction process may involve modal aliasing, optimal wavelet base issues, and inconsistencies between the lightning strike distance and the fault point. These factors have the potential to impact the effectiveness of recognition. This paper presents a method for identifying lightning strike faults by utilizing Kullback–Leibler (KL) divergence enhanced Variational Mode Decomposition (VMD) and Symmetric Geometry Mode Decomposition (SGMD) improved with Permutation Entropy (PE) to address the aforementioned issues. A model of a 220 kV overhead line is constructed using real faults to replicate scenarios of winding strike, counterstrike, and short circuit. The three-phase voltage is chosen and then subjected to Karenbaren decoupling in order to transform it into zero mode, line mode 1, and line mode 2. The zero-mode voltage is decomposed using KL-VMD and PE-SGMD methods, and the lightning identification criteria are developed based on various transient energy ratios. The research findings demonstrate that the criteria effectively differentiate between winding strike, counterstrike, and short-circuit faults, thus confirming the accuracy and efficacy of the lightning fault identification criteria utilizing KL-VMD and PE-SGMD.
Integrated and Hybrid Processes for the Treatment of Actual Wastewaters Containing Micropollutants: A Review on Recent Advances
Authors: Asheghmoalla Mina , Mehrvar Mehrab
Published: February 2024

The global concern regarding the release of micropollutants (MPs) into the environment has grown significantly. Considerable amounts of persistent micropollutants are present in industrial discharges. Depending solely on a singular treatment approach is inadequate for the effective removal of MPs from wastewater due to their complex composition. The performance of different treatment methods to meet the discharge standards has been widely studied. These efforts are classified as hybrid and sequential processes. Despite their adequate performance, the optimization and industrial application of these methods could be challenging and costly. This review focuses on integrated (sequential) and hybrid processes for MP removal from actual wastewater. Furthermore, to provide a thorough grasp of the treatment approaches, the operational conditions, the source of wastewater containing MPs, and its characteristics are detailed. It is concluded that the optimal sequence to achieve the removal of MPs involves biological treatment followed by an advanced oxidation process (AOP) with a final passage through an activated carbon column. To refine this process further, a membrane unit could be added based on the desired effluent quality. Nevertheless, considering practical feasibility, this study identifies specific areas requiring additional research to implement this integrated treatment strategy effectively.