bluuebunny/crossref_metadata_2025 huggingface dataset:
Small (≤5 cm) soft tissue tumors of the extremity and superficial trunk: MRI features and demographic characteristics associated with malignancy
Authors: Jin Kiok , Yoon Min A , Kim Do Yeon
Published: December 2022

Background

Although a substantial proportion of small soft tissue tumors are malignant, magnetic resonance imaging (MRI) features and demographic characteristics associated with these tumors have not been well described.

Purpose

To investigate the MRI features and demographic characteristics associated with small (≤5 cm) malignant soft tissue tumors, and to identify independent predictors that allow differentiation of small benign and malignant soft tissue tumors.

Material and Methods

This retrospective study evaluated patients who underwent surgical excision of small soft tissue tumors of the extremities and superficial trunk, and preoperative contrast-enhanced MRI. Seven MRI findings (tumor depth, tumor-fascia relationship, heterogeneity of signal intensity, necrosis, peritumoral edema, peritumoral enhancement, and margin) and two demographic parameters (age and sex) were included in univariate and multivariate logistic regression analyses to identify independent predictors of small malignant soft tissue tumors.

Results

A total of 221 patients (102 men; mean age=45.6 ± 17.6 years) with 72 malignant and 149 benign tumors were included. In the univariate analysis, peritumoral edema (odds ratio [OR] = 3.854; P < 0.001) and peritumoral enhancement (OR = 3.966; P < 0.001) and patient age (≥46 years) (OR = 2.154; P = 0.009) were significantly associated with malignancy. Multivariate analysis showed that peritumoral enhancement on MRI (OR = 3.728; P < 0.001) and patient age (≥46 years) (OR = 1.907; P = 0.036) were independent predictors of malignancy. The combination of these two parameters showed accuracy of 75.1%, sensitivity of 55.6%, and specificity of 84.6% to predict malignancy.

Conclusion

Among several MRI and demographic features, the presence of peritumoral enhancement on MRI and patient age (≥46 years) were independent predictors of malignancy in small soft tissue tumors.
Nomogram for Predicting Asphalt Pavement Roughness After Preventive Maintenance Based on Long-Term Pavement Performance Data
Authors: Zhang Miaomiao , Gong Hongren , Ma Yuetan , Jiang Xi , Huang Baoshan
Published: December 2022

Relative effectiveness has been used as the primary criterion when choosing pavement preventive maintenance (PM) treatments; however, the most effective treatment is not necessarily the most cost-effective treatment because of the higher cost. Cost-effectiveness should be determined by predictive models of PM-treated pavement performance, which has rarely been investigated. In this study, a predictive model for post-treatment pavement roughness—as defined by international roughness index (IRI)—was established utilizing the data from the Long-Term Pavement Performance Program (LTPP) Specific Pavement Study 3 (SPS-3). The generalized least squares (GLS) model was employed to improve the predictive performance by exploiting the characteristics of LTPP panel data. A nomogram was also provided to help highway agencies manually obtain the predicted post-treatment IRI values. Results show that post-treatment IRI was significantly higher in dry and non-freeze areas than in other climate areas. The effect of pavement structure on post-treatment IRI was time-dependent; it was insignificant at the beginning and gradually increased after 4 years. Although post-treatment IRI was affected by pavement structures and climate, the relative effectiveness of different PM treatments was only related to the pre-treatment IRI. Thin overlay significantly improved the pavement IRI, and when pre-treatment IRI was 2.0 m/km, the post-treatment IRI of the thin overlay would be reduced to 0.6 times that of the control. However, there was no significant difference in pavement IRI between different seal treatments.
Bringing Radiology Education to a New Reality: A Pilot Study of Using Virtual Reality as a Remote Educational Tool
Authors: Wu Yuhao , Mondal Prosanta , Stewart Matthew , Ngo Richard , Burbridge Brent
Published: December 2022

Purpose: We investigated virtual reality (VR) during a 2-week, undergraduate, radiology elective to determine if it improved learning outcomes and user satisfaction. Methods: Eighteen students enrolled between August 2021 and February 2022. Each student had a collaborative Zoom teaching session with a preceptor using a Picture Archive and Communications System (PACS)-like viewing system Online DICOM Image Navigator (ODIN), followed by a teaching session using a VR, Digital Imaging and Communications in Medicine (DICOM) viewer (SieVRt). After each teaching session, the students independently reviewed 8 imaging cases and completed case related questions. The students completed a survey, rating their subjective experiences using ODIN and SieVRt. Results: There was no difference in total test scores between the two learning strategies. However, students did perform statistically better on two of five questions designed to test the detection/measurement capabilities of SieVRt vs ODIN. Students stated that they preferred using SieVRt over ODIN and agreed that they were able to view subtle imaging findings and abnormalities better using SieVRt. However, students found that some of the functions of SieVRt (measuring angles/lengths, and multitasking) were difficult. There were technical challenges with VR and minor undesirable physical effects (dizziness, nausea, etc.). Conclusions: Virtual reality has the potential to enhance radiology education by providing an immersive and engaging experience. Objectively, students were able to perform two tasks better with SieVRt. Subjectively, the VR platform received favourable reviews from students for a variety of features. There were reported technical and physical challenges related to using VR. Future developments in VR systems should focus on improving the user experience.
Prescribing Patterns of Direct-Acting Oral Anticoagulants in Patients With Atrial Fibrillation and Chronic Kidney Disease: A Retrospective Cohort Analysis
Authors: Reyes Jorge L. , Herzog Charles A. , Yan Heng , Roetker Nicholas S. , Wetmore James B.
Published: December 2022

Background:

The association of patient and prescriber characteristics with use of warfarin versus direct-acting oral anticoagulants (DOACs) in patients with atrial fibrillation (AF) and chronic kidney disease (CKD) is not well studied.

Methods:

The 20% Centers for Medicare & Medicaid Services Parts A, B, and D claims data from 2010 to 2017 were used to identify patients with stage 3, 4, or 5 CKD and AF who received a DOAC (apixaban, dabigatran, rivaroxaban) or warfarin. Prescribers were categorized as cardiologists, primary care providers (PCPs), and others. Using logistic regression, we estimated odds ratios (ORs) for the association of baseline characteristics and prescriber specialty with first use of a DOAC, relative to warfarin.

Results:

We identified 22,739 individuals with CKD who were newly initiated on oral anticoagulation for AF. New DOAC prescriptions increased from 490 in 2011 to 3261 in 2017, and displaced warfarin over time (1849, 2011; 945, 2017). By Q4 of 2014, cardiologists prescribed DOACs as initial treatment more frequently than warfarin, but non-cardiologists did not do so until 2015. As of 2017, apixaban was the most widely prescribed anticoagulant, comprising 56% and 50% of prescriptions by cardiologists and non-cardiologists, respectively. PCPs (OR 0.54, 0.51-0.58) and other providers (OR 0.55, 0.51-0.59) were less likely than cardiologists to prescribe DOACs.

Conclusions:

DOAC prescriptions, particularly apixaban, increased over time and gradually displaced warfarin. The total number of patients with AF and CKD receiving anticoagulation increased over time. Cardiologists increased DOAC prescriptions more rapidly than non-cardiologists.
AAV2-hAADC (Eladocagene Exuparvovec) Biodistribution and Expression: Superiority of Intraputaminal versus Intracerebroventricular and Intrathecal (Lumbar) Routes of Administration
Authors: Compton David R , DeMarco Steven J , Yalamanchili Padmaja
Published: December 2022

Aromatic L-amino acid decarboxylase deficiency is a genetic disorder of enzyme loss with decreased neurotransmitter synthesis, and it is characterized by symptoms of impaired motor development and cognitive function, hypotonia, dystonia, and oculogyric crises. Though symptomatic severity varies, the majority of patients experience severe motor impairments, including an inability to sit, stand, or walk. One approved therapy for Aromatic L-amino acid decarboxylase deficiency involves intraputaminal delivery of an adeno-associated virus packaging the human Aromatic L-amino acid decarboxylase enzyme (hAADC) cDNA. The objective of this study in monkeys was to determine the acceptability of ICV/IT as minimally invasive dosing options by evaluating hAADC biodistribution and expression following intraputaminal, intracerebroventricular (ICV), or intrathecal (IT, lumbar) administration. Results show that all routes produced comparable CSF transgene levels and were well-tolerated. The intraputaminal route yielded the highest levels of transgene-derived mRNA expression in the putamen, caudate, and globus pallidus, while expression levels in the spinal cord and dorsal root ganglia (DRG, a target of special toxicological concern) were undetectable. In contrast, the highest transgene levels in ICV/IT groups were observed in the spinal cord and DRG, but levels were too low to result in expression in the putamen, caudate, and globus pallidus. Unlike ICV/IT, the intraputaminal route produced no transgene in blood, suggesting a lower likelihood of off-target toxicities. Additionally, intraputaminal dosing resulted in the lowest anti-AAV2 antibody (anti-drug antibody) levels. Together, these data demonstrate the superiority of intraputaminal administration over ICV/IT routes in achieving AAV2-hAADC transgene DNA distribution and mRNA expression in target therapeutic areas while minimizing risk of toxicity.
Specificity and sensitivity of the social communication questionnaire lifetime screening tool for autism spectrum disorder in a UK CAMHS service
Authors: Staton Amelia , Dawson David , Moghaddam Nima , McGrath Barbara
Published: December 2022

Introduction

The Social Communication Questionnaire is used to identify children and young people (CYP) who may require formal ASD assessment. However, there is a paucity of research on its utility in Children and Adolescent Mental Health Services. This evaluation aimed to determine the sensitivity and specificity of the Social Communication Questionnaire (SCQ) in a UK, Midlands CAMHS service.

Method

Forty young people (mean age 13.75 years) were screened using the caregiver reported SCQ before completing ‘gold standard’ assessment.

Results

The SCQ had a sensitivity of 80% and a specificity of 25.7%. ROC curve analysis indicated low diagnostic accuracy. Differences in predictive accuracy of SCQ and diagnostic standard were statistically significant ( p < 0.0001).

Conclusion

This evaluation builds on previous research suggesting that the SCQ may not be an efficient screening tool in CAMHS settings.
Intelligent defect location of a U-shaped boom using helical guided waves
Authors: Lu Zhaojing , Liu Zenghua , Jiang Wenshuo , Wu Bin , He Cunfu
Published: December 2022

Truck cranes are indispensable heavy-duty loading and unloading equipment in industrial production. A boom is the key load-bearing component of a truck crane, and its health has a vital influence on a crane’s lifting performance and safety in production. Therefore, it is urgent to develop a structural health monitoring (SHM) method for a boom structure. In this research, an improved intelligent defect location algorithm based on helical guided waves is applied to the defect detection of a U-shaped boom. The improved intelligent algorithm is a defect location algorithm based on the ellipse imaging principle, which combines an evolutionary algorithm with a K-means algorithm and can identify the location of defects through the distribution of individuals. According to the propagation characteristics of the helical guided waves in the structure of a U-shaped boom, an optimization scheme for the improved intelligent defect location algorithm is proposed that fully considers the fact that the group velocity of the helical guided waves varies with the wall thickness, and the corresponding group velocity is used to accurately calculate the arrival time of each initial individual to improve the accuracy of the defect location. Numerical simulations and experiments are conducted to verify the effectiveness of the proposed improved intelligent defect location algorithm in the defect location algorithm. When only one group velocity is considered, the improved intelligent defect location algorithm is in good agreement with the elliptical imaging algorithm. When the group velocities for different wall thicknesses are fully considered, the detection results of the optimized intelligent defect location algorithm have a higher resolution. This optimization algorithm provides a tool for the SHM of a U-shaped boom based on guided waves.
Intravascular Ultrasound in Treating Iliac Vein Compression With Endovascular Stenting: A Necessary Tool for Optimal Outcomes
Authors: Melian Christina M. , Giannopoulos Stefanos , Volteas Panagiotis , Virvilis Dimitrios
Published: December 2022

Symptomatic compression of the left common iliac vein between the right common iliac artery and spinal vertebrae is referred to as May-Thurner Syndrome (MTS). Atypical cases of MTS including compression of the left external iliac vein, right iliac vein or the inferior vena cava can also coexist and cause double vein compression. Current literature suggests that endovascular treatment including thrombolysis, thrombectomy, venoplasty and stent placement to correct the mechanical obstruction together with anticoagulation therapy is safe and a reasonable management for patients with MTS. Intravascular ultrasound (IVUS) can aid in the diagnosis and the operative planning of MTS, especially regarding sizing and precise deployment of venous stents. Here we present 2 unique atypical cases of MTS with double left iliac vein compression treated endovascularly with stent placement along the common and external iliac vein with the assistance of IVUS.
Cohesion and collective efficacy as antecedents and team performance as an outcome of team resilience in team sports
Authors: López-Gajardo Miguel A. , García-Calvo Tomás , González-Ponce Inmaculada , Díaz-García Jesús , Leo Francisco M.
Published: December 2022

This research project, composed of two studies, aimed to analyse various antecedents and outcomes of team resilience. Study 1, which involved 394 sports team players ( Mage  =  20.41, SD  =  5.23), cross-sectionally examined the relationship between group cohesion and collective efficacy with team resilience, and the mediating effect of collective efficacy on the cohesion-team resilience relationship, using players’ perceptions assessed through questionnaires. Study 2, which involved 434 players ( Mage  =  21.53, SD  =  5.57) from collective sports, aimed to longitudinally corroborate the relationship between cohesion (Time 1) and collective efficacy (Time 1) with team resilience (Time 2), and to examine perceived team performance (Time 2) as a consequence of team resilience, again evaluated through questionnaires. Results showed that task cohesion, social cohesion and collective efficacy in Studies 1 and 2 were positively related to resilience characteristics. Also, task cohesion was negatively related to vulnerability under pressure in both studies. Collective efficacy was also shown to be an antecedent of vulnerability under pressure in Study 1 and a mediator of the relationship between task cohesion and resilience characteristics in both studies. Lastly, team resilience was a predictor of perceived team performance at the end of the season in Study 2. Thus, the findings provide new empirical evidence of the antecedents and outcomes of team resilience in the field of sports psychology. Coaches and practitioners should develop group cohesion and collective efficacy in training sessions and matches during the season to achieve more resilient and less vulnerable teams.
Men’s sexual desire, and why women often don’t recognize it? Prevalence of erectile dysfunction and associated factors among diabetes mellitus patients attending Mettu Karl Comprehensive Specialized Hospital: A cross-sectional study
Authors: Bekele Firomsa , Fantahun Lelise , Garbessa Bayisa , Dekosa Fili
Published: December 2022

Objective:

The study aimed to assess the magnitude and predictors of erectile dysfunction at Mettu Karl Comprehensive Specialized Hospital.

Methods:

Male diabetic patients visiting chronic care follow-up at Mettu Karl Comprehensive Specialized Hospital were face-to-face interviewed as part of a retrospective cross-sectional study from April 6, 2022 to May 6, 2022. SPSS version 21 for Windows was used to enter and analyze the data for the descriptive statistics. Following that, a binary logistic regression and a multivariate logistic regression model were used. Based on a p-value of 0.05, variables that had an independent link with sexual dysfunction were found. The adjusted odds ratio and its associated 95% confidence interval were also used to analyze the direction and intensity of the link.

Results:

A total of 307 diabetic men participated in the study, with a mean age of (52.74, 16.16) and a mean duration of (5.94, 2.974). In this study, 252 people (82.1%) experienced erectile dysfunction, with mild, moderate, and severe cases represented by 30%, 38%, and 14.1% of respondents, respectively. There were statistically significant associations between erectile dysfunction and age (adjusted odds ratio: 4.8, 95% confidence interval: 1.27–18.32), type of diabetes (adjusted odds ratio: 5.8, 95% confidence interval: 1.96–17.73), and comorbidities (adjusted odds ratio: 0.32, 95% confidence interval: 0.15–0.71).

Conclusion:

This study showed that the prevalence rate of erectile dysfunction is high in Mettu Karl Comprehensive Specialized Hospital. Age, type of diabetes mellitus, and the presence of comorbidity were factors associated with erectile dysfunction. Therefore, assessment and management of erectile dysfunction in the diabetic clinic should be part of routine medical care during follow-up visits with diabetic patients.
Conformity to Heterosexual Engagement Proposal Scripts: Do Same-Sex Couples Benefit?
Authors: Cast Alicia D. , Schweingruber David S.
Published: December 2022

Previous research on engagement proposals finds that conformity to a traditional engagement proposal script legitimates the couple in the eyes of the larger community. This script, however, has long been held to be heteronormative, enforcing norms about not only marriage but also gender. Using a sample of college students at a midwestern university, the authors explore whether conformity to a heterosexual engagement proposal script transfers the same social legitimacy to same-sex couples. Using vignettes, participants were asked to rate the strength and likelihood of staying together of three types of hypothetical couples (female-male, female-female, and male-male) on the basis of the traditionality of the engagement proposal script. Results suggest that relationships that conform to a traditional engagement proposal script are evaluated as being stronger and more likely to last, regardless of the sex category composition of the couple.
Energy Expenditure and Substrate Utilization with Hands-Free Crutches Compared to Conventional Lower-Extremity Injury Mobility Devices
Authors: Hackney Kyle J. , Bradley Adam P. , Roehl Alexis S. , McGrath Ryan , Smith Joseph
Published: December 2022

Background:

A Hands-Free crutch (HFC) is a relatively new device that can be used during the nonweightbearing period to increase mobility. The primary aim of this investigation was to examine aerobic oxygen consumption (V.o2) and substrate utilization with HFC compared to conventional ambulation devices as well as normal ambulation. A secondary purpose was to quantify perceived exertion, pain, and performance during each ambulation condition.

Methods:

Forty participants completed 4 separate 10-minute ambulation conditions around a rectangular course. The order of the ambulation conditions was randomized and consisted of (1) walking, (2) medical knee scooter (MKS), (3) HFC, and (4) axillary crutch (AC). Indirect calorimetry was used to determine V.o2 and the respiratory exchange ratio (RER), an indicator of substrate utilization. Perceived exertion and pain were also assessed using questionnaires.

Results:

All mobility devices significantly elevated V.o2 (+35%) compared to walking (13.14 ± 1.70 mL/kg/min; P < .001). AC had significantly greater V.o2 requirements (20.26 ± 2.62 mL/kg/min) compared to both the MKS (15.28 ± 2.29 mL/kg/min; P < .001) and HFC (15.88 ± 2.03 mL/kg/min; P < .001). There was no difference in average V.o2 between MKS and HFC ( P = .368). Compared to walking (0.78 ± 0.43), RER was significantly elevated in MKS (0.81 ± 0.05, P < .001) and AC (0.84 ± 0.06, P < .001), but not in HFC (0.79 ± 0.04, P = .350). RPE and pain were elevated in all ambulatory conditions (all P values <.001). Pain was significantly greater in AC compared with MKS ( P < .001) and HFC ( P < .001).

Conclusion:

HFC and MKS share similar V.o2 requirements over a 10-minute ambulation interval and are below those needed in AC. Substrate utilization in HFC was similar to regular walking with a greater reliance on lipid utilization for energy as evidenced by a lower RER. Exertion and pain scores were the most tolerable in HFC and MKS.

Level of Evidence:

Level II, prospective comparative study.
Using Extreme Pedagogy to Enhance Entrepreneurship Education
Authors: Silberman Dave , Aguinis Herman , Carpenter Rob E.
Published: December 2022

We address the ongoing concern that entrepreneurship education (EE) is not preparing students sufficiently well for jobs in the 21st-century. We argue that many criticisms leveled against EE for not effectively addressing the entrepreneurial skills gap are due in part to EE’s emphasis on the roles of specific stakeholder groups separately (i.e., universities and their leaders, instructors, students, potential employers) rather than a shared focus on developing valuable graduates. Stated differently, there are competing and conflicting “ownerships” over the entrepreneurial skills gap. We enhance current pedagogical methods by offering a learning innovation called extreme pedagogy. Extreme pedagogy takes place when all stakeholders have a collective intention and ownership in producing graduates with relevant entrepreneurial skills. We describe extreme pedagogy’s conceptual foundation based on psychological ownership theory, the effective of use of extreme ownership in military contexts, and the role of universities and their leaders, instructors, students, and potential employers in the implementation of extreme pedagogy. We then summarize themes from a workshop involving entrepreneurial leaders across industries on EE challenges and the role of extreme pedagogy in addressing them. We close by describing anticipated benefits of extreme pedagogy for all EE stakeholders.
Extract antibody and antigen names from biomedical literature
Authors: Dinh Thuy Trang , Vo-Chanh Trang Phuong , Nguyen Chau , Huynh Viet Quoc , Vo Nam , Nguyen Hoang Duc
Published: December 2022

Background

The roles of antibody and antigen are indispensable in targeted diagnosis, therapy, and biomedical discovery. On top of that, massive numbers of new scientific articles about antibodies and/or antigens are published each year, which is a precious knowledge resource but has yet been exploited to its full potential. We, therefore, aim to develop a biomedical natural language processing tool that can automatically identify antibody and antigen entities from articles.

Results

We first annotated an antibody-antigen corpus including 3210 relevant PubMed abstracts using a semi-automatic approach. The Inter-Annotator Agreement score of 3 annotators ranges from 91.46 to 94.31%, indicating that the annotations are consistent and the corpus is reliable. We then used the corpus to develop and optimize BiLSTM-CRF-based and BioBERT-based models. The models achieved overall F1 scores of 62.49% and 81.44%, respectively, which showed potential for newly studied entities. The two models served as foundation for development of a named entity recognition (NER) tool that automatically recognizes antibody and antigen names from biomedical literature.

Conclusions

Our antibody-antigen NER models enable users to automatically extract antibody and antigen names from scientific articles without manually scanning through vast amounts of data and information in the literature. The output of NER can be used to automatically populate antibody-antigen databases, support antibody validation, and facilitate researchers with the most appropriate antibodies of interest. The packaged NER model is available athttps://github.com/TrangDinh44/ABAG_BioBERT.git.
An endophytic Schizophyllum commune possessing antioxidant activity exhibits genoprotective and organprotective effects in fresh water fish Channa punctatus exposed to bisphenol A
Authors: Sharma Avinash , Chauhan Pooja , Sharma Khushboo , Kalotra Vishali , Kaur Anupam , Chadha Pooja , Kaur Sukhraj , Kaur Amarjeet
Published: December 2022

Background

Oxidative stress is responsible for the onset of several chronic and degenerative diseases. Exogenous supply of antioxidants is reported to neutralize the effects of oxidative stress. Several synthetic antioxidants suffer from various side effects which necessitates the exploration of antioxidant compounds from natural sources. Endophytic fungi residing in the plants are gaining the attention of researchers as a source of novel antioxidants. Majority of the research conducted so far on endophytic fungi has been restricted to the members of phylum ascomycota. Basidiomycota, inspite of their immense bioactive potential remain relatively unexploited. This study aimed to assess the ameliorative effects of an endophyticSchizophyllum commune(basidiomycetous fungus) against oxidative stress associated altered antioxidant levels, genotoxicity and cellular damage to different organs in bisphenol A exposed fresh water fishChanna punctatus.

Results

Good antioxidant and genoprotective potential was exhibited byS. communeextract inin vitrostudies conducted using different antioxidant, DNA damage protection, and cytokinesis blocked micronuclei assays. In vivo studies were performed in fresh water fishChanna punctatusexposed to bisphenol A. A significant decrease in the considered parameters for DNA damage (% micronuclei and comet assay) were recorded in fish treated withS. communeextract on comparison with untreated bisphenol A exposed group. TheS. communeextract treated fish also exhibited an increase in the level of antioxidant enzymesviz. catalase, superoxide dismutase and glutathione reductase as well as histoprotective effect on various organs. GC-MS analysis revealed the presence of 3-n-propyl-2,4-pentanedione, n-heptadecanol-1, trans-geranylgeraniol, 3-ethyl-2-pentadecanone, 1-heneicosanol and squalene as some of the compounds inS. communeextract.

Conclusion

The study highlights the significance of an endophytic basidiomycetous fungusS. communeas a source of antioxidant compounds with possible therapeutic potential.
Exploring combat stress exposure effects on burn pain in a female rodent model
Authors: Strain Misty M. , Tongkhuya Sirima , Wienandt Nathan , Alsadoon Farah , Chavez Roger , Daniels Jamar , Garza Thomas , Trevino Alex V. , Wells Kenney , Stark Thomas , Clifford John , Sosanya Natasha M.
Published: December 2022

In the military, constant physiological and psychological stress encountered by Soldiers can lead to development of the combat and operational stress reaction (COSR), which can effect pain management. Similar effects are seen in other populations subjected to high levels of stress. Using a model of COSR, our lab recently showed that four weeks of stress prior to an injury increases pain sensitivity in male rats. With the roles of women in the military expanding and recent studies indicating sex differences in stress and pain processing, this study sought to investigate how different amounts of prior stress exposure affects thermal injury-induced mechanosensitivity in a female rat model of COSR. Adult female Sprague Dawley rats were exposed to the unpredictable combat stress (UPCS) procedure for either 2 or 4 weeks. The UPCS procedure included exposure to one stressor each day for four days. The stressors include: (1) sound stress for 30 min, (2) restraint stress for 4 h, (3) cold stress for 4 h, and (4) forced swim stress for 15 min. The order of stressors was randomized weekly. Mechanical and thermal sensitivity was tested twice weekly. After the UPCS procedure, a sub-set of rats received a thermal injury while under anesthesia. The development of mechanical allodynia and thermal hyperalgesia was examined for 14 days post-burn. UPCS exposure increased mechanosensitivity after two weeks. Interestingly, with more stress exposure, females seemed to habituate to the stress, causing the stress-induced changes in mechanosensitivity to decrease by week three of UPCS. If thermal injury induction occurred during peak stress-induced mechanosensitivity, after two weeks, this resulted in increased mechanical allodynia in the injured hind paw compared to thermal injury alone. This data indicates a susceptibility to increased nociceptive sensitization when injury is sustained at peak stress reactivity. Additionally, this data indicates a sex difference in the timing of peak stress. Post-mortem examination of the prefrontal cortex (PFC) showed altered expression of p-TrkB in 4-week stressed animals given a thermal injury, suggesting a compensatory mechanism. Future work will examine treatment options for preventing stress-induced pain to maintain the effectiveness and readiness of the Warfighter.
Evolution and functional analysis of the GRAS family genes in six Rosaceae species
Authors: Bai Yibo , Liu Hui , Zhu Kaikai , Cheng Zong-Ming
Published: December 2022

Background

GRAS genes formed one of the important transcription factor gene families in plants, had been identified in several plant species. The family genes were involved in plant growth, development, and stress resistance. However, the comparative analysis of GRAS genes in Rosaceae species was insufficient.

Results

In this study, a total of 333 GRAS genes were identified in six Rosaceae species, including 51 in strawberry (Fragaria vesca), 78 in apple (Malus domestica), 41 in black raspberry (Rubus occidentalis), 59 in European pear (Pyrus communis), 56 in Chinese rose (Rosa chinensis), and 48 in peach (Prunus persica). Motif analysis showed the VHIID domain, SAW motif, LR I region, and PFYRE motif were considerably conserved in the six Rosaceae species. All GRAS genes were divided into 10 subgroups according to phylogenetic analysis. A total of 15 species-specific duplicated clades and 3 lineage-specific duplicated clades were identified in six Rosaceae species. Chromosomal localization presented the uneven distribution of GRAS genes in six Rosaceae species. Duplication events contributed to the expression of the GRAS genes, and Ka/Ks analysis suggested the purification selection as a major force during the evolution process in six Rosaceae species. Cis-acting elements and GO analysis revealed that most of the GRAS genes were associated with various environmental stress in six Rosaceae species. Coexpression network analysis showed the mutual regulatory relationship between GRAS and bZIP genes, suggesting the ability of the GRAS gene to regulate abiotic stress in woodland strawberry. The expression pattern elucidated the transcriptional levels of FvGRAS genes in various tissues and the drought and salt stress in woodland strawberry, which were verified by RT-qPCR analysis.

Conclusions

The evolution and functional analysis of GRAS genes provided insights into the further understanding of GRAS genes on the abiotic stress of Rosaceae species.
Comparative transcriptome profiling of resistant and susceptible foxtail millet responses to Sclerospora graminicola infection
Authors: Wang He , Han Yanqing , Wu Caijuan , Zhang Baojun , Zhao Yaofei , Zhu Jiao , Han Yuanhuai , Wang Jianming
Published: December 2022

Background

Downy mildew of foxtail millet, which is caused by the biotrophic oomyceteSclerospora graminicola(Sacc.) Schroeter, is one of the most disruptive diseases. The foxtail millet-S. graminicolainteraction is largely unexplored. Transcriptome sequencing technology can help to reveal the interaction mechanism between foxtail millet and its pathogens.

Results

Transmission electron microscopy observations of leaves infected withS. graminicolashowed that the structures of organelles in the host cells gradually became deformed and damaged, or even disappeared from the 3- to 7-leaf stages. However, organelles in the leaves of resistant variety were rarely damaged. Moreover, the activities of seven cell wall degrading enzymes in resistant and susceptible varieties were also quite different after pathogen induction and most of enzymes activities were significantly higher in the susceptible variety JG21 than in the resistant variety G1 at all stages. Subsequently, we compared the transcriptional profiles between the G1 and JG21 in response toS. graminicolainfection at 3-, 5-, and 7-leaf stages using RNA-Seq technology. A total of 473 and 1433 differentially expressed genes (DEGs) were identified in the resistant and susceptible varieties, respectively. The pathway analysis of the DEGs showed that the highly enriched categories were related to glutathione metabolism, plant hormone signalling, phenylalanine metabolism, and cutin, suberin and wax biosynthesis. Some defence-related genes were also revealed in the DEGs, including leucine-rich protein kinase, Ser/Thr protein kinase, peroxidase, cell wall degrading enzymes, laccases and auxin response genes. Our results also confirmed the linkage of transcriptomic data with qRT-PCR data. In particular, LRR protein kinase encoded by Seita.8G131800, Ser/Thr protein kinase encoded by Seita.2G024900 and Seita. 2G024800, which have played an essential resistant role during the infection byS. graminicola.

Conclusions

Transcriptome sequencing revealed that host resistance toS. graminicolawas likely due to the activation of defence-related genes, such as leucine-rich protein kinase and Ser/Thr protein kinase. Our study identified pathways and genes that contribute to the understanding of the interaction between foxtail millet andS. graminicolaat the transcriptomic level.The results will help us better understand the resistance mechanism of foxtail millet againstS. graminicola.
Magnetic controlled capsule endoscope (MCCE)‘s diagnostic performance for H. pylori infection status based on the Kyoto classification of gastritis
Authors: Xi Sun , Jing Liu , Lili Wu , Tingting Li , Jun Li , Ming Wang , Zhiqiang Wang , Peng Jin
Published: December 2022

Background

Previous studies have shown that the Kyoto classification of gastritis can accurately predict H. pylori infection status on conventional gastroscopy. The aim of this study was to test whether the Kyoto classification of gastritis applies well to magnetic controlled capsule endoscopy (MCCE).

Methods

We consecutively recruited 227 participants who underwent both MCCE and urea breath tests (UBTs). Two physicians who were blinded to the UBT results independently made the diagnosis of H. pylori infection status according to 10 findings listed in the Kyoto classification of gastritis after reviewing MCCE images. We also developed 2 predictive models to assess H. pylori infection status by combining these 10 findings.

Results

The MCCE’s overall diagnostic accuracy for H. pylori infection status was 80.2%. The sensitivity, specificity and diagnostic odds ratio (DOR) for current infection were 89.4%, 90.1% and 77.1, respectively. Major specific findings were mucosal swelling and spotty redness for current infection, regular arrangement of collecting venules (RAC), streak redness, fundic gland polyp (FGP) for noninfection, and map-like redness for past-infection. In the two prediction models, the area under the curve (AUC) values for predicting noninfection and current infection were 84.7 and 84.9, respectively.

Conclusions

The Kyoto classification of gastritis applied well to MCCE. H. pylori infection status could be accurately assessed on MCCE according to the Kyoto classification of gastritis.
Association of pain and quality of life among middle-aged and older adults of India
Authors: Goyal Amit Kumar , Mohanty Sanjay K
Published: December 2022

Background

India is passing through a phase of demographic and epidemiological transition where ageing and chronic morbidities are being more common. Though studies have examined the prevalence and risk factors of pain and other chronic morbidities, nationally representative research examining the association of pain and quality of life (QoL) is limited in India. This study examines the association between pain and QoL among middle-aged and older adults in India.

Methods

This study uses the data from wave 1 of the Longitudinal Ageing Study in India (LASI) conducted in 2017-18. This study is restricted to 58,328 individuals from all states (except Sikkim), aged 45 years and above. The quality of life is measured in 6 domains (physical, psychological, social, environment, general health and life satisfaction) with 21 variables that range from 0 to 100. The principal component analysis was used to generate a composite score of QoL and the multiple linear regression was used to show the association between pain and quality of life.

Results

It is estimated that approximately 37% of Indian middle-aged and older populations were often troubled with pain. Pain prevalence increase with age and is more common among older adults aged 75 + years (43.37%; 95% CI, 40.95–45.80), and female (41.38%; 95% CI, 39.36–43.39). The average QoL score among those with pain was 81.6 compared to 85.2 among those without pain. QoL was lower among elderly age 75 and above, females, rural residents and illiterates. Controlling for socio-demographic factors, pain reduces the QoL by 2.57 points (β= −2.57; 95% CI, −3.02 - −2.11).

Conclusion

Pain reduces the quality of life among middle-aged adults and older adults in India. This evidence could potentially help the policymakers to consider pain as a significant determinant of quality of life in India.
Interactions between the apolipoprotein E4 gene and modifiable risk factors for cognitive impairment: a nationally representative panel study
Authors: Kolli Ajay , Zhou Yunshu , Chung Grace , Ware Erin B. , Langa Kenneth M. , Ehrlich Joshua R.
Published: December 2022

Background

Few studies using rigorous clinical diagnosis have considered whether associations with cognitive decline are potentiated by interactions between genetic and modifiable risk factors. Given the increasing burden of cognitive impairment (CI) and dementia, we assessed whether Apolipoprotein E ε4 (APOE4) genotype status modifies the association between incident CI and key modifiable risk factors .

Methods

Older adults (70+) in the US were included. APOE4 status was genotyped. Risk factors for CI were self-reported. Cognitive status (normal, CI, or dementia) was assigned by clinical consensus panel. In eight separate Cox proportional hazard models, we assessed for interactions between APOE4 status and other CI risk factors.

Result

The analytical sample included 181 participants (mean age 77.7 years; 45.9% male). APOE4 was independently associated with a greater hazard of CI in each model (Hazard Ratios [HR] between 1.81–2.66, p < 0.05) except the model evaluating educational attainment (HR 1.65, p = 0.40). The joint effects of APOE4 and high school education or less (HR 2.25, 95% CI: 1.40–3.60, p < 0.001), hypertension (HR 2.46, 95% CI: 1.28–4.73, p = 0.007), elevated depressive symptoms (HR 5.09, 95% CI: 2.59–10.02, p < 0.001), hearing loss (HR 3.44, 95% CI: 1.87–6.33, p < 0.0001), vision impairment (HR 5.14, 95% CI: 2.31–11.43, p < 0.001), smoking (HR 2.35, 95% CI: 1.24–4.47, p = 0.009), or obesity (HR 3.80, 95% CI: 2.11–6.85, p < 0.001) were associated with the hazard of incident CIND (compared to no genetic or modifiable risk factor) in separate models. The joint effect of Apolipoprotein ε4 and type 2 diabetes was not associated with CIND (HR 1.58, 95% CI: 0.67–2.48, p = 0.44).

Discussion

The combination of APOE4 and selected modifiable risk factors conveys a stronger association with incident CI than either type of risk factor alone.
Frozen embryo transfer in the menstrual cycle after moderate-severe ovarian hyperstimulation syndrome: a retrospective analysis
Authors: Yang Yulu , Zhu Damin , Wang Qiushuang , Ma Cong , Li Danyang , Wang Jing , Zhou Ping , Wei Zhaolian , Peng Xiaoqing , Cao Yunxia , Xu Xiaofeng
Published: December 2022

Background

Ovarian hyperstimulation syndrome (OHSS) is a rare but serious complication of controlled ovarian stimulation. Frozen-embryo transfer (ET) is prompted to be performed in the next menstrual cycles after cancellation of fresh-ET after occurrence of OHSS. However, effects of frozen-ET in the second menstrual cycle have never been investigated. Therefore, this study aimed to assess this in the menstrual cycle after OHSS.

Methods

The OHSS group included 342 women with moderate-severe OHSS who underwent the first frozen-ET in the second menstrual cycle in the First Affiliated Hospital of Anhui Medical University from June 2018 to September 2019. A total of 342 women without OHSS who received frozen-ET in the second menstrual cycle were selected as control group matched by age, body mass index, fertility history, ovulation induction scheme. Uni- and multi-variable conditional logistic regression was used to estimate the association between moderate-severe OHSS and pregnancy outcomes.

Results

There were no significant differences in maternal outcomes (miscarriage, preterm birth and pregnancy complications including gestational diabetes mellitus, pregnancy-induced hypertension, placenta previa, premature rupture of membranes and postpartum hemorrhage) and in neonatal outcome (birth-weight and body length, neonatal congenital diseases and other complications) between the two groups in either uni- or multi-variable models.

Conclusions

Frozen-ET in the menstrual cycle after OHSS has similar maternal and neonatal outcomes as in women without OHSS. This study indicates that frozen-ET could be performed in the second menstrual cycle in women who recovered from moderate-severe OHSS.
Adult abuse and poor prognosis in Taiwan, 2000–2015: a cohort study
Authors: Yu Pi-Ching , Chiang Ya-Hsuan , Huang Shi-Hao , Chung Ren-Jei , Lin Iau-Jin , Wang Bing-Long , Yu Chia-Peng , Chou Yu-Ching , Sun Chien-An , Tang Shih-En , Huang Yao-Ching , Chien Wu-Chien , Chiang Chun-Hsien
Published: December 2022

Background

To investigate the risk of poor prognosis regarding schizophrenic disorders, psychotic disorders, suicide, self-inflicted injury, and mortality after adult violence from 2000 to 2015 in Taiwan.

Methods

This study used data from National Health Insurance Research Database (NHIRD) on outpatient, emergency, and inpatient visits for two million people enrolled in the National Health Insurance (NHI) from 2000 to 2015. The case study defined ICD-9 diagnosis code N code 995.8 (abused adult) or E code E960-E969 (homicide and intentional injury of another). It analyzed first-time violence in adults aged 18–64 years (study group). 1:4 ratio was matched with injury and non-violent patients (control group). The paired variables were sex, age (± 1 year), pre-exposure to the Charlson comorbidity index, and year of medical treatment. Statistical analysis was conducted using SAS 9.4 and Cox regression for data analysis.

Results

In total, 8,726 individuals experienced violence (case group) while34,904 did not experienced violence (control group) over 15 years. The prevalence of poor prognosis among victims of violence was 25.4/104, 31.3/104, 10.5/10,4 and 104.6/104 for schizophrenic disorders, psychotic disorders, suicide or self-inflicted injury and mortality, respectively. Among adults, the risks of suicide or self-inflicted injury, schizophrenic disorders, psychotic disorders, and mortality after exposure to violence (average 9 years) were 6.87-, 5.63-, 4.10-, and 2.50-times (p < 0.01), respectively, compared with those without violence. Among males, the risks were 5.66-, 3.85-, 3.59- and 2.51-times higher, respectively, than those without violence (p < 0.01), and they were 21.93-, 5.57-, 4.60- and 2.46-times higher than those without violence (p < 0.01) among females.

Conclusion

The risk of poor prognosis regarding schizophrenic disorders, psychotic disorders, suicide, or self-inflicted injury and mortality after adult violence was higher than in those who have not experienced a violent injury. Adults at the highest risk for violent suicide or self-inflicted injuries due to exposure to violent injuries —males were at risk for schizophrenia and females were at risk for suicide or self-inflicted injuries. Therefore, it is necessary for social workers and medical personnel to pay attention to the psychological status of victims of violence.
Associations between smoking status and involvement of personal and professional relations among individuals reporting symptoms related to a diagnosis of lung cancer: a population-based study
Authors: Vognsgaard Frederik , Sætre Lisa Maria Sele , Rasmussen Sanne , Jarbøl Dorte Ejg
Published: December 2022

Background

Smoking is the leading cause of lung cancer, but individuals who currently smoke seek healthcare less frequently. This study of individuals reporting symptoms related to diagnosis of lung cancer has the following aims: 1) to explore the involvement of personal and professional relations; 2) to analyse whether age, sex and smoking status are associated with involving personal and professional relations; and 3) to analyse whether involving a personal relation is associated with healthcare-seeking.

Methods

Data was extracted from a Danish population-based survey from 2012 with 100,000 randomly selected invitees 20 years or older. We describe the involvement of personal and professional relations among individuals experiencing four predefined symptoms indicative of lung cancer: prolonged coughing, prolonged hoarseness, shortness of breath and haemoptysis, either alone or in combination. Using multivariate logistic regression, we analyse the associations between involving personal or professional relations and various covariates (sex, age, smoking status). Moreover, we analyse the association between involving a personal relation and healthcare-seeking.

Results

A total of 35,958 individuals over 40 years old completed the questionnaire. Of these, 5,869 individuals reported at least one lung cancer symptom. A higher percentage of participants with prolonged hoarseness and prolonged coughing reported no involvement of personal and professional relations (27.6% and 22.7%, respectively) compared to shortness of breath (12.4%). The most involved personal and professional relations were the spouse (46.2–62.5%) and the general practitioner (GP) (31.3–54.5%), respectively. Women and individuals in the oldest age group had higher odds of involving personal and professional relations. Individuals who currently smoke involved all relations less frequently than individuals who formerly,- and never smoked. Odds of contacting the GP or another doctor were three to seven-fold higher when a personal relation was also involved.

Conclusion

Women and the oldest age group had higher odds of involving relations, whereas individuals who currently smoked tended to be less likely to contact any personal or professional relations. Involving a personal relation was associated with higher odds of healthcare-seeking. The findings could be useful for GPs in terms of identifying patients at risk of postponing relevant healthcare-seeking with potential lung cancer symptoms.