bluuebunny/crossref_metadata_2025 huggingface dataset:
A Pan-Cancer Analysis of Predictive Methylation Signatures of Response to Cancer Immunotherapy
Authors: Xu Bingxiang , Lu Mingjie , Yan Linlin , Ge Minghui , Ren Yong , Wang Ru , Shu Yongqian , Hou Lin , Guo Hao
Published: December 2021

Recently, tumor immunotherapy based on immune checkpoint inhibitors (ICI) has been introduced and widely adopted for various tumor types. Nevertheless, tumor immunotherapy has a few drawbacks, including significant uncertainty of outcome, the possibility of severe immune-related adverse events for patients receiving such treatments, and the lack of effective biomarkers to determine the ICI treatments’ responsiveness. DNA methylation profiles were recently identified as an indicator of the tumor immune microenvironment. They serve as a potential hot spot for predicting responses to ICI treatment for their stability and convenience of measurement by liquid biopsy. We demonstrated the possibility of DNA methylation profiles as a predictor for responses to the ICI treatments at the pan-cancer level by analyzing DNA methylation profiles considered responsive and non-responsive to the treatments. An SVM model was built based on this differential analysis in the pan-cancer levels. The performance of the model was then assessed both at the pan-cancer level and in specific tumor types. It was also compared to the existing gene expression profile-based method. DNA methylation profiles were shown to be predictable for the responses to the ICI treatments in the TCGA cases in pan-cancer levels. The proposed SVM model was shown to have high performance in pan-cancer and specific cancer types. This performance was comparable to that of gene expression profile-based one. The combination of the two models had even higher performance, indicating the potential complementarity of the DNA methylation and gene expression profiles in the prediction of ICI treatment responses.
Agreement and Repeatability of Central and Peripheral Refraction by One Novel Multispectral-Based Refractor
Authors: Lu Weicong , Ji Rongyuan , Ding Wenzhi , Tian Yuyin , Long Keli , Guo Zhen , Leng Lin
Published: December 2021

Purpose: To evaluate the repeatability of a multispectral-based refractor in central and peripheral refraction measurement, and to assess the agreement of such measurements with objective refraction (OR) and subjective refraction (SR) in patients with myopia.

Methods: A total of 60 subjects were recruited in this prospective research. Patients were divided into three groups according to the refractive error. Next, the central and peripheral refraction parameters were measured using multispectral refractive tomography (MRT) before and after cycloplegia. In addition, OR and SR measurements were also performed. The intraobserver repeatability was analyzed using within-subject standard deviation (Sw), test–retest repeatability (TRT), and intraclass correlation coefficient (ICC). Agreement was evaluated using Bland-Altman plot and 95% limits of agreement (LoA).

Results: The ICC value of central and peripheral refraction were all higher than 0.97 with or without cycloplegia. The peripheral refraction in the nasal, temporal, superior, and inferior quadrants was slightly worse than other parameters, with the largest error interval being 1.43 D. The 95% LoA of the central refraction and OR or SR ranged from −0.89 to 0.88 D and −1.24 to 1.16 D without cycloplegia, respectively, and from −0.80 to 0.42 D and −1.39 to −0.84 D under cycloplegia, respectively.

Conclusions: The novel multispectral refraction topography demonstrated good repeatability in central and peripheral refraction. However, the refraction in the nasal, temporal, superior, and inferior quadrants were not as good as that of central and circle peripheral refraction.
The Strength of the Medial Olivocochlear Reflex in Chinchillas Is Associated With Delayed Response Performance in a Visual Discrimination Task With Vocalizations as Distractors
Authors: Vicencio-Jimenez Sergio , Bucci-Mansilla Giuliana , Bowen Macarena , Terreros Gonzalo , Morales-Zepeda David , Robles Luis , Délano Paul H.
Published: December 2021

The ability to perceive the world is not merely a passive process but depends on sensorimotor loops and interactions that guide and actively bias our sensory systems. Understanding which and how cognitive processes participate in this active sensing is still an open question. In this context, the auditory system presents itself as an attractive model for this purpose as it features an efferent control network that projects from the cortex to subcortical nuclei and even to the sensory epithelium itself. This efferent system can regulate the cochlear amplifier sensitivity through medial olivocochlear (MOC) neurons located in the brainstem. The ability to suppress irrelevant sounds during selective attention to visual stimuli is one of the functions that have been attributed to this system. MOC neurons are also directly activated by sounds through a brainstem reflex circuit, a response linked to the ability to suppress auditory stimuli during visual attention. Human studies have suggested that MOC neurons are also recruited by other cognitive functions, such as working memory and predictability. The aim of this research was to explore whether cognitive processes related to delayed responses in a visual discrimination task were associated with MOC function. In this behavioral condition, chinchillas held their responses for more than 2.5 s after visual stimulus offset, with and without auditory distractors, and the accuracy of these responses was correlated with the magnitude of the MOC reflex. We found that the animals’ performance decreased in presence of auditory distractors and that the results observed in MOC reflex could predict this performance. The individual MOC strength correlated with behavioral performance during delayed responses with auditory distractors, but not without them. These results in chinchillas, suggest that MOC neurons are also recruited by other cognitive functions, such as working memory.
Real-Time Edge Neuromorphic Tasting From Chemical Microsensor Arrays
Authors: LeBow Nicholas , Rueckauer Bodo , Sun Pengfei , Rovira Meritxell , Jiménez-Jorquera Cecilia , Liu Shih-Chii , Margarit-Taulé Josep Maria
Published: December 2021

Liquid analysis is key to track conformity with the strict process quality standards of sectors like food, beverage, and chemical manufacturing. In order to analyse product qualities online and at the very point of interest, automated monitoring systems must satisfy strong requirements in terms of miniaturization, energy autonomy, and real time operation. Toward this goal, we present the first implementation of artificial taste running on neuromorphic hardware for continuous edge monitoring applications. We used a solid-state electrochemical microsensor array to acquire multivariate, time-varying chemical measurements, employed temporal filtering to enhance sensor readout dynamics, and deployed a rate-based, deep convolutional spiking neural network to efficiently fuse the electrochemical sensor data. To evaluate performance we created MicroBeTa (Microsensor Beverage Tasting), a new dataset for beverage classification incorporating 7 h of temporal recordings performed over 3 days, including sensor drifts and sensor replacements. Our implementation of artificial taste is 15× more energy efficient on inference tasks than similar convolutional architectures running on other commercial, low power edge-AI inference devices, achieving over 178× lower latencies than the sampling period of the sensor readout, and high accuracy (97%) on a single Intel Loihi neuromorphic research processor included in a USB stick form factor.
Total Flavonoids of Chuju Decrease Oxidative Stress and Cell Apoptosis in Ischemic Stroke Rats: Network and Experimental Analyses
Authors: Wang Cong , Chen Hao , Jiang Hui-hui , Mao Bin-bin , Yu Hao
Published: December 2021

Background: Pharmacological research results showed that total flavonoids of Chuju (TFCJ) could be used to treat acute myocardial ischemia and myocardial ischemia-reperfusion injury. In this study, we explored the protective effect of TFCJ on ischemic stroke (IS) in the IS rat model. We hypothesized that TFCJ might exert its neuroprotective effects by suppressing apoptosis and oxidative stress that are closely related to PI3K/Akt/mTOR signaling pathway.

Method: TFCJ (10, 20, and 40 mg/kg) was administered for 7 days. Rats (260 ± 20 g) were subjected to middle cerebral artery occlusion (MCAO) for 2 h and reperfusion for 24 h. The neuroprotective effect of TFCJ was substantiated in terms of neurological deficits, oxidative stress (superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde), pathomorphological changes (HE staining and TUNEL staining), and neurobehavioral functions in the rats. Then, we employed network pharmacology to reveal the potential mechanism of TFCJ against IS. Western blot was used to determine the levels of PI3K/AKT/mTOR pathway proteins. The expression of BCL-2, BAX, and cleaved-Caspase-3 was also measured by Western blots and RT-PCR.

Results: The histopathological assessment showed that TFCJ reduced MCAO-induced brain damage. Besides, TFCJ exerted a protective role in MCAO rats by alleviating cell apoptosis and oxidative stress. Network pharmacology showed that TFCJ might be used against IS through the PI3K/AKT signaling pathway. TFCJ reduced cell apoptosis and oxidative stress by increasing the level of p-AKT and p-mTOR in MCAO rats, while the effect of TFCJ was significantly reversed when applying LY294002 (PI3k inhibitor).

Conclusion: These results indicated that TFCJ might decrease oxidative stress and apoptosis that are closely related to PI3K/Akt/mTOR pathway in IS. TFCJ is a promising authentic traditional Chinese medicine for the management of IS.
Ketogenic Diet for Obese COVID-19 Patients: Is Respiratory Disease a Contraindication? A Narrative Review of the Literature on Ketogenic Diet and Respiratory Function
Authors: Gangitano Elena , Tozzi Rossella , Mariani Stefania , Lenzi Andrea , Gnessi Lucio , Lubrano Carla
Published: December 2021

Morbid obese people are more likely to contract SARS-CoV-2 infection and its most severe complications, as need for mechanical ventilation. Ketogenic Diet (KD) is able to induce a fast weight loss preserving lean mass and is particularly interesting as a preventive measure in obese patients. Moreover, KD has anti-inflammatory and immune-modulating properties, which may help in preventing the cytokine storm in infected patients. Respiratory failure is actually considered a contraindication for VLCKD, a very-low calorie form of KD, but in the literature there are some data reporting beneficial effects on respiratory parameters from ketogenic and low-carbohydrate high-fat diets. KD may be helpful in reducing ventilatory requirements in respiratory patients, so it should be considered in specifically addressed clinical trials as an adjuvant therapy for obese patients infected with SARS-CoV-2.
Dexamethasone is Associated With a Lower Risk of the Progression of Thoracic Aortic Calcification in Breast Cancer Survivors
Authors: Lei Juan , Liu Aiting , Ma Yujia , Shi Guangzi , Han Feng , Jiang Wenlong , Zhou Yongqiao , Zhang Chao , Liu Yimin , Huang Xiaobo , Huang Hui , Chen Jie
Published: December 2021

Background and Purpose: Breast cancer survivors have an increased cardiovascular risk, and vascular calcification is the pathological basis of cardiovascular disease. Some factors that affect the progression of thoracic aortic calcification (TAC) in survivors are unclear, and this study aims to explore the relationship between dexamethasone or radiotherapy and the progression of TAC in survivors.

Materials and Methods: This study included 189 female patients with breast cancer, and they were divided into the progression and non-progression TAC groups. Radiation or dexamethasone doses, and related laboratory parameters were collected.

Results: The cumulative dose of dexamethasone was higher [40 (10–180) mg versus 180 (80–270) mg, p < 0.001], and the cycle was longer [4 (1–6) cycles versus 6 (4–8) cycles, p < 0.001] in the non-progression TAC group. The cumulative dose (r = −0.303, p < 0.001) and cycle (r = −0.357, p < 0.001) of dexamethasone were negatively correlated with the level of increased TAC Agatston scores in survivors. Logistic regression analysis showed that dexamethasone was a protective factor for the progression of TAC (p = 0.029, odds ratio = 0.263, 95% confidence interval = 0.08–0.872). However, there wasn’t significant relationship between radiotherapy, radiation dose, follow-up time and the progression of TAC (all p > 0.05). In addition, aorta volume was positively correlated with the level of increased TAC Agatston scores in intensity modulated radiation therapy (r = 0.460, p < 0.001).

Conclusion: Dexamethasone is associated with a lower risk of the progression of TAC in breast cancer survivors, and there’s no correlation between radiotherapy and progression of TAC, but the aorta volume may be a predictor of the severity of progression of TAC.
Vincristine-Induced Peripheral Neuropathy in Childhood Acute Lymphoblastic Leukemia: Genetic Variation as a Potential Risk Factor
Authors: Yang Qing-Yan , Hu Ya-Hui , Guo Hong-Li , Xia Ying , Zhang Yong , Fang Wei-Rong , Li Yun-Man , Xu Jing , Chen Feng , Wang Yong-Ren , Wang Teng-Fei
Published: December 2021

Vincristine (VCR) is the first-line chemotherapeutic medication often co-administered with other drugs to treat childhood acute lymphoblastic leukemia. Dose-dependent neurotoxicity is the main factor restricting VCR’s clinical application. VCR-induced peripheral neuropathy (VIPN) sometimes results in dose reduction or omission, leading to clinical complications or affecting the patient’s quality of life. With regard to the genetic basis of drug responses, preemptive pharmacogenomic testing and simultaneous blood level monitoring could be helpful for the transformation of various findings into individualized therapies. In this review, we discussed the potential associations between genetic variants in genes contributing to the pharmacokinetics/pharmacodynamics of VCR and VIPN incidence and severity in patients with acute lymphoblastic leukemia. Of note, genetic variants in the CEP72 gene have great potential to be translated into clinical practice. Such a genetic biomarker may help clinicians diagnose VIPN earlier. Besides, genetic variants in other genes, such as CYP3A5, ABCB1, ABCC1, ABCC2, TTPA, ACTG1, CAPG, SYNE2, SLC5A7, COCH, and MRPL47, have been reported to be associated with the VIPN, but more evidence is needed to validate the findings in the future. In fact, a variety of complex factors jointly determine the VIPN. In implementing precision medicine, the combination of genetic, environmental, and personal variables, along with therapeutic drug monitoring, will allow for a better understanding of the mechanisms of VIPN, improving the effectiveness of VCR treatment, reducing adverse reactions, and improving patients’ quality of life.
Impact of Qi-Invigorating Traditional Chinese Medicines on Diffuse Large B Cell Lymphoma Based on Network Pharmacology and Experimental Validation
Authors: Huang Qian , Lin Jinkun , Huang Surong , Shen Jianzhen
Published: December 2021

Background: It has been verified that deficiency of Qi, a fundamental substance supporting daily activities according to the Traditional Chinese Medicine theory, is an important symptom of cancer. Qi-invigorating herbs can inhibit cancer development through promoting apoptosis and improving cancer microenvironment. In this study, we explored the potential mechanisms of Qi-invigorating herbs in diffuse large B cell lymphoma (DLBCL) through network pharmacology and in vitro experiment.

Methods: Active ingredients of Qi-invigorating herbs were predicted from the Traditional Chinese Medicine Systems Pharmacology Database. Potential targets were obtained via the SwissTargetPrediction and STITCH databases. Target genes of DLBCL were obtained through the PubMed, the gene-disease associations and the Malacards databases. Overlapping genes between DLBCL and each Qi-invigorating herb were collected. Hub genes were subsequently screened via Cytoscape. The Gene Ontology and pathway enrichment analyses were performed using the DAVID database. Molecular docking was performed among active ingredients and hub genes. Hub genes linked with survival and tumor microenvironment were analyzed through the GEPIA 2.0 and TIMER 2.0 databases, respectively. Additionally, in vitro experiment was performed to verify the roles of common hub genes.

Results: Through data mining, 14, 4, 22, 22, 35, 2, 36 genes were filtered as targets of Ginseng Radix et Rhizoma, Panacis Quinquefolii Radix, Codonopsis Radix, Pseudostellariae Radix, Astragali Radix, Dioscoreae Rhizoma, Glycyrrhizae Radix et Rhizoma for DLBCL treatment, respectively. Then besides Panacis Quinquefolii Radix and Dioscoreae Rhizoma, 1,14, 10, 14,13 hub genes were selected, respectively. Molecular docking studies indicated that active ingredients could stably bind to the pockets of hub proteins. CASP3, CDK1, AKT1 and MAPK3 were predicted as common hub genes. However, through experimental verification, only CASP3 was considered as the common target of Qi-invigorating herbs on DLBCL apoptosis. Furthermore, the TIMER2.0 database showed that Qi-invigorating herbs might act on DLBCL microenvironment through their target genes. Tumor-associated neutrophils may be main target cells of DLBCL treated by Qi-invigorating herbs.

Conclusion: Our results support the effects of Qi-invigorating herbs on DLBCL. Hub genes and immune infiltrating cells provided the molecular basis for each Qi-invigorating herb acting on DLBCL.
Single-Larva RNA Sequencing Identifies Markers of Copper Toxicity and Exposure in Early Mytilus californianus Larvae
Authors: Hall Megan R. , Gracey Andrew Y.
Published: December 2021

One of the challenges facing efforts to generate molecular biomarkers for toxins is distinguishing between markers that are indicative of exposure and markers that provide evidence of the effects of toxicity. Phenotypic anchoring provides an approach to help segregate markers into these categories based on some phenotypic index of toxicity. Here we leveraged the mussel embryo-larval toxicity assay in which toxicity is estimated by the fraction of larvae that exhibit an abnormal morphology, to isolate subsets of larvae that were abnormal and thus showed evidence of copper-toxicity, versus others that while exposed to copper exhibited normal morphology. Mussel larvae reared under control conditions or in the presence of increasing levels of copper (3–15 μg/L Cu2+) were physically sorted according to whether their morphology was normal or abnormal, and then profiled using RNAseq. Supervised differential expression analysis identified sets of genes whose differential expression was specific to the pools of abnormal larvae versus normal larvae, providing putative markers of copper toxicity versus exposure. Markers of copper exposure and copper-induced abnormality were involved in many of the same pathways, including development, shell formation, cell adhesion, and oxidative stress, yet unique markers were detected in each gene set. Markers of effect appeared to be more resolving between phenotypes at the lower copper concentration, while markers of exposure were informative at both copper concentrations.
Effect of Intensity on Changes in Cardiac Autonomic Control of Heart Rate and Arterial Stiffness After Equated Continuous Running Training Programs
Authors: Soltani Mohammad , Baluchi Masoud Jokar , Boullosa Daniel , Daraei Ali , Doyle-Baker Patricia K. , Saeidi Ayoub , Knechtle Beat , Dehbaghi Kambiz Moradi , Mollabashi Shirin Shirzad , VanDusseldorp Trisha A. , Zouhal Hassane
Published: December 2021

Background: It is well known that exercise training has positive effects on both cardiac autonomic function and arterial stiffness (AS). However, it is not clear that which exercise training variables, intensity or volume, or both, play a crucial role in this regard. This study investigates the chronic effects of high-volume moderate-intensity training (HVMIT) and low-volume high-intensity training (LVHIT) on heart rate variability (HRV) and AS in sedentary adult men.

Materials and Methods: Notably, 45 males (age: 42 ± 5.7 years) were randomly assigned to a control (n = 15), HVMIT (n = 15), or LVHIT (n = 15). The HVMIT group ran three times per week on a treadmill at 50–60% of VO2max for 45–60 min, while the LVHIT trained at 70–85% of VO2max for 25–40 min. Both training protocols were equated by caloric expenditure. HRV, pulse wave velocity (PWV), hemodynamic variables, and body composition were measured before and after 12 weeks.

Results: Both protocols (i.e., HVMIT and LVHIT) significantly increased the SD of normal sinus beat intervals (SDNN) and high-frequency (HF) bands (p < 0.05) after 12 weeks. Whereas the low-frequency (LF)-HF ratio decreased significantly in both training protocols (p < 0.05); however, these changes were significantly greater in the LVHIT protocol (p < 0.05). Furthermore, the root mean square of successive RR interval differences (RMSSD) significantly increased only in the LVHIT (p < 0.05). Moreover, a significant decrease in LF and PWV was only observed following the LVHIT protocol (p < 0.05). Some measures of HRV and PWV were significantly correlated (r = 0.275–0.559; p < 0.05).

Conclusion: These results show that the LVHIT protocol was more efficient for improving HRV variables and PWV than the HVMIT protocol after 12 weeks of continuous running training. Interestingly, changes in some HRV parameters were related to changes in PWV. Further studies should elaborate on the link between central and peripheral cardiovascular adaptations after continuous and intermittent training regimens differing in intensity.
Impact of Huanglongbing Pathogen Infection on the Amino Acid Composition in Both Citrus Plants and the Asian Citrus Psyllid
Authors: Zhang Li-He , Ren Su-Li , Su Zheng-Qin , Xu Pei-Ping , Ou Da , Wang Li-Jun , Sang Wen , Qiu Bao-Li
Published: December 2021

The Asian citrus psyllid (ACP) Diaphorina citri is the main vector of the pathogen Candidatus Liberibacter asiaticus (CLas), which is the causal agent of citrus Huanglongbing disease. Feeding by both ACP nymphs and adults on host plants allows them to obtain nutrition. Therefore, the nutritional content within the plant phloem is of much importance for the development and reproduction of ACP. The infection by pathogenic microbiomes may affect the amino acid contents of their host plants and then indirectly affect the biology of sap-feeding insects. In this study, we investigated the amino acid contents and their proportions in both CLas-infected and CLas-free citrus plants, ACP adults, and also in honeydew produced by ACP nymphs. Results showed that infection by CLas had a large impact on the amino acid species and proportion in all the tested target plants, ACP adults, and in the honeydew of ACP nymphs. The content of total amino acids in CLas-infected citrus was much higher than that of CLas-free citrus. However, CLas infection significantly reduced the proportion of essential amino acids (EAAs) in these plants. When feeding on CLas-infected citrus plants, ACP adults absorbed less total amino acids than those adults feeding on healthy plants, but the proportion of EAAs was significantly higher when they fed on CLas-infected citrus plants. The proportion of EAAs also significantly increased in the honeydew secreted by ACP nymphs that fed on CLas-infected citrus plants. However, EAA detection in the honeydew of ACP nymphs indicated that the utilization rate of EAAs by CLas positive ACP nymphs was reduced. Our study has revealed that CLas infection significantly affects the contents, proportion, and utilization efficiency of different amino acids in citrus plants, ACP adults, and nymphs, leading to a developmental pattern of ACP that is more conducive to CLas transmission.
MiRNA126 – RGS16 – CXCL12 Cascade as a Potential Mechanism of Acute Exercise-Induced Precursor Cell Mobilization
Authors: Schmid Michelle , Martins Helena Caria , Schratt Gerhard , Kröpfl Julia M. , Spengler Christina M.
Published: December 2021

Acute exercise enhances circulating stem and precursor cells (CPCs) in the peripheral blood. The responsible mechanisms and molecular pathways, however, have not been fully identified. The aim of the present study was to investigate a pathway related to elevated levels of apoptotic peripheral blood mononuclear cells (MNCs) and their secretome. An increased uptake of miRNA126 in MNCs was suggested to lead to reduced levels of RGS16 mRNA and, in turn, an enhanced translation and secretion of CXCL12. Eighteen healthy, young men underwent two identical incremental cycling exercises of which the first served as control while the second was preceded by a 7-day-long antioxidative supplementation. Blood samples were collected at baseline (−10min) and several time points after exercise (0, 30, 90, 180, and 270min). Relative concentrations of miRNA126 in MNCs and CXCL12 levels in plasma were determined at all time points while RGS16 mRNA was assessed in MNCs at baseline and 30min after exercise. CXCL12 increased after exercise and strongly correlated with CPC numbers. MiRNA126 increased 30min and, to a lesser extent, also 180 and 270min after exercise but only with supplementation. RGS16 mRNA decreased 30min after exercise independent of the intervention. The amount of RGS16 mRNA inversely correlated with levels of miRNA126, but not with plasma CXCL12. In conclusion, even though plasma CXCL12 correlated with CPC numbers, the increase in CXCL12 cannot be explained by the increased concentration of miRNA126 and lower RGS16 mRNA in MNCs that would have allowed for an enhanced translation of CXCL12.

Clinical Trial Registration: ClinicalTrials.gov, NCT03747913. Registered 20 November 2018, https://clinicaltrials.gov/ct2/show/NCT03747913.
Melatonin Promotes the Chilling Tolerance of Cucumber Seedlings by Regulating Antioxidant System and Relieving Photoinhibition
Authors: Zhang Xiaowei , Feng Yiqing , Jing Tongtong , Liu Xutao , Ai Xizhen , Bi Huangai
Published: December 2021

Chilling adversely affects the photosynthesis of thermophilic plants, which further leads to a decline in growth and yield. The role of melatonin (MT) in the stress response of plants has been investigated, while the mechanisms by which MT regulates the chilling tolerance of chilling-sensitive cucumber remain unclear. This study demonstrated that MT positively regulated the chilling tolerance of cucumber seedlings and that 1.0 μmol⋅L–1 was the optimum concentration, of which the chilling injury index, electrolyte leakage (EL), and malondialdehyde (MDA) were the lowest, while growth was the highest among all treatments. MT triggered the activity and expression of antioxidant enzymes, which in turn decreased hydrogen peroxide (H2O2) and superoxide anion (O2⋅–) accumulation caused by chilling stress. Meanwhile, MT attenuated the chilling-induced decrease, in the net photosynthetic rate (Pn) and promoted photoprotection for both photosystem II (PSII) and photosystem I (PSI), regarding the higher maximum quantum efficiency of PSII (Fv/Fm), actual photochemical efficiency (ΦPSII), the content of active P700 (ΔI/I0), and photosynthetic electron transport. The proteome analysis and western blot data revealed that MT upregulated the protein levels of PSI reaction center subunits (PsaD, PsaE, PsaF, PsaH, and PsaN), PSII-associated protein PsbA (D1), and ribulose-1,5-bisphosphate carboxylase or oxygenase large subunit (RBCL) and Rubisco activase (RCA). These results suggest that MT enhances the chilling tolerance of cucumber through the activation of antioxidant enzymes and the induction of key PSI-, PSII-related and carbon assimilation genes, which finally alleviates damage to the photosynthetic apparatus and decreases oxidative damage to cucumber seedlings under chilling stress.
Teaching Children’s Motor Skills for Team Games Through Guided Discovery: How Constraints Enhance Learning
Authors: Newell Karl M. , Rovegno Inez
Published: December 2021

In this paper we examine the role of instructional strategies as constraints within a discovery learning framework for the teaching of open skill team ball games to elementary school-aged children. The cohesive and adaptive integration of constraints (individual, environment, and task) by practitioners of movement and physical activity (instructor, teacher, coach) is proposed as the pathway to exploiting the effectiveness of guided discovery learning. The qualitative analysis of the practical instantiations of this framework by expert teachers is examined with respect to the learning of open skill team invasion games (e.g., basketball, soccer). The primary constraints to action in this learning-teaching developmental framework are coordinated so as to keep the self-organization of skill development (movement pattern and tactics) continually evolving, while preserving the child’s motivation and enjoyment for the expanding repertoire and performance capacity of his/her perceptual-motor skills. In this open skill and elementary school age-related context, generalityandspecificity are both necessary and complementary in the expression of task, skill and practice influences on motor learning and performance.
The Differential Impact of Mystery in Nature on Attention: An Oculometric Study
Authors: Marois Alexandre , Charbonneau Brooke , Szolosi Andrew M. , Watson Jason M.
Published: December 2021

Nature exposure can provide benefits on stress, health and cognitive performance. According to Attention Restoration Theory (ART), the positive impact of nature on cognition is mainly driven by fascination. Fascinating properties of nature such as water or a winding hiking trail may capture involuntary attention, allowing the directed form of attention to rest and to recover. This claim has been supported by studies relying on eye-tracking measures of attention deployment, comparing exposure to urban and nature settings. Yet, recent studies have shown that promoting higher engagement with a nature setting can improve restorative benefits, hence challenging ART’s view that voluntary attention is resting. Besides, recent evidence published by Szolosi et al. (2014) suggests that voluntary attention may be involved during exposure to high-mystery nature images which they showed as having greater potential for attention restoration. The current study explored how exposure to nature images of different scenic qualities in mystery (and restoration potential) could impact the engagement of attention. To do so, participants were shown nature images characterized by either low or high mystery properties (with allegedly low or high restoration potential, respectively) and were asked to evaluate their fascination and aesthetic levels. Concurrently, an eye tracker collected measures of pupil size, fixations and spontaneous blinks as indices of attentional engagement. Results showed that high-mystery nature images had higher engagement than low-mystery images as supported by the larger pupil dilations, the higher number of fixations and the reduced number of blinks and durations of fixations. Taken together, these results challenge ART’s view that directed attention is merely resting during exposure to restorative nature and offer new hypotheses on potential mechanisms underlying attention restoration.
Does an Imitation Strategy Promote Long-Term Firm Growth in a Dynamic Environment? A Meta-Analysis
Authors: Peng Huatao , Zhou Chen , Sadowski Bert M. , Sun Tingshu
Published: December 2021

The increasing number of successful latecomer enterprises has led to a growing research interest in the area, but there is a lack of consensus in academic circles on the relationship between imitation strategy and firm growth. While some enterprises achieved sustainable growth based on an imitation strategy, others withdrew from the market soon after their initial market entry. In this context, this meta-analysis synthesizes empirical findings including 23 independent samples (N = 66,110) to obtain evidence and explore the extent to which an imitation strategy affects firm growth. Moreover, by further examining the moderating effects of industry conditions, country-specific factors, and performance time horizons, this research also aims to address a complementary research question: in which context is imitation strategy more beneficial for the firm growth? We found that an imitation strategy is more effective in promoting firm growth in low-tech industries than in high-tech industries and in non-OECD countries than in OECD countries. It fosters the short-term performance rather than the long-term performance of a firm. Our research findings are meaningful for enterprises to choose an appropriate imitation strategy according to their unique attributes, enabling sustainable growth in a dynamic environment.
Liaison Psychiatry During the Peak of the Coronavirus Pandemic: A Description of Referrals and Interventions
Authors: López-Atanes Mayte , González-Briceño Juan Pablo , Abeal-Adham Adrián , Fuertes-Soriano Sara , Cabezas-Garduño Janire , Peña-Rotella Álvar , Sáenz-Herrero Margarita
Published: December 2021

Introduction: The novel coronavirus SARS-CoV-2 belongs to the coronavirus family, a group of viruses that can cause upper respiratory infections in humans. Among other symptoms, it can present as an asymptomatic infection or as a more severe disease requiring hospitalization. Neuropsychiatric symptoms have been described in the acute phase of the illness and as long-term repercussions. We describe the characteristics and interventions in those COVID-19 patients referred to our liaison psychiatry service.

Materials and Methods: This is a cross-sectional descriptive study. This study was carried out within the Department of Psychiatry of Cruces University Hospital (Basque Country, Spain). Data from each psychiatric consultation within our consultation-liaison service were consecutively obtained for 1 month from March 17 to April 17, 2020. We recruited data regarding clinical and referral characteristics and psychiatric interventions.

Results: Of a total of 721 SARS-CoV-2 hospitalizations, 43 (5.6%) patients were referred to our psychiatry liaison service. The median age was 61 years old, and 62.8% were women. The infectious disease department was the most frequent petitioner (37.2%), and the most common reason for referral was patient anxiety (25.6%). A total of 67.4% of patients received psychological counseling and 55.8% received some pharmacological approach, with a median of 3.7 visits/calls per patient. In addition, 20.3% needed a medication switch due to potential interactions between psychotropics and drugs used to treat SARS-CoV-2.

Discussion: In our study, up to 5.6% of SARS-CoV-2 hospitalized patients needed a psychiatric evaluation, especially for anxiety and mood symptoms. Psychosocial factors associated with the pandemic, drugs used to treat the infection, or a direct causative effect of the virus may explain our findings.
Bidirectional Associations Between Depressive and Anxiety Symptoms and Loneliness During the COVID-19 Pandemic: Dynamic Panel Models With Fixed Effects
Authors: McDowell Cillian P. , Meyer Jacob D. , Russell Daniel W. , Sue Brower Cassandra , Lansing Jeni , Herring Matthew P.
Published: December 2021

Background: Understanding the direction and magnitude of mental health-loneliness associations across time is important to understand how best to prevent and treat mental health and loneliness. This study used weekly data collected over 8 weeks throughout the COVID-19 pandemic to expand previous findings and using dynamic panel models with fixed effects which account for all time-invariant confounding and reverse causation.

Methods: Prospective data on a convenience and snowball sample from all 50 US states and the District of Colombia (n = 2,361 with ≥2 responses, 63.8% female; 76% retention rate) were collected weekly via online survey at nine consecutive timepoints (April 3–June 3, 2020). Anxiety and depressive symptoms and loneliness were assessed at each timepoint and participants reported the COVID-19 containment strategies they were following. Dynamic panel models with fixed effects examined bidirectional associations between anxiety and depressive symptoms and loneliness, and associations of COVID-19 containment strategies with these outcomes.

Results: Depressive symptoms were associated with small increases in both anxiety symptoms (β = 0.065, 95% CI = 0.022–0.109; p = 0.004) and loneliness (β = 0.019, 0.008–0.030; p = 0.001) at the subsequent timepoint. Anxiety symptoms were associated with a small subsequent increase in loneliness (β = 0.014, 0.003–0.025; p = 0.015) but not depressive symptoms (β = 0.025, −0.020–0.070; p = 0.281). Loneliness was strongly associated with subsequent increases in both depressive (β = 0.309, 0.159–0.459; p < 0.001) and anxiety (β = 0.301, 0.165–0.436; p < 0.001) symptoms. Compared to social distancing, adhering to stay-at-home orders or quarantining were not associated with anxiety and depressive symptoms or loneliness (both p ≥ 0.095).

Conclusions: High loneliness may be a key risk factor for the development of future anxiety or depressive symptoms, underscoring the need to combat or prevent loneliness both throughout and beyond the COVID-19 pandemic. COVID-19 containment strategies were not associated with mental health, indicating that other factors may explain previous reports of mental health deterioration throughout the pandemic.
Anxiety, Anger and Depression Amongst Low-Income Earners in Southwestern Uganda During the COVID-19 Total Lockdown
Authors: Archibong Victor , Usman Ibe Michael , Kasozi Keneth Iceland , Aigbogun Eric Osamudiamwen , Josiah Ifie , Monima Ann Lemuel , Ssebuufu Robinson , Chekwech Gaudencia , Terkimbi Swase Dominic , Owoisinke Okon , Mbiydzenyuy Ngala Elvis , Adeoye Azeez , Aruwa Joshua Ojodale , Afodun Adam Moyosore , Odoma Saidi , Ssempijja Fred , Ayikobua Emmanuel Tiyo , Ayuba John Tabakwot , Nankya Viola , Onongha Comfort , Henry Sussan , Matama Kevin , Yusuf Helen , Nalugo Halima , MacLeod Ewan , Welburn Susan Christina
Published: December 2021

Background: Low-income earners are particularly vulnerable to mental health, consequence of the coronavirus disease 2019 (COVID-19) lockdown restrictions, due to a temporary or permanent loss of income and livelihood, coupled with government-enforced measures of social distancing. This study evaluates the mental health status among low-income earners in southwestern Uganda during the first total COVID-19 lockdown in Uganda.

Methods: A cross-sectional descriptive study was undertaken amongst earners whose income falls below the poverty threshold. Two hundred and fifty-three (n = 253) male and female low-income earners between the ages of 18 and 60 years of age were recruited to the study. Modified generalized anxiety disorder (GAD-7), Spielberger's State-Trait Anger Expression Inventory-2 (STAXI-2), and Beck Depression Inventory (BDI) tools as appropriate were used to assess anxiety, anger, and depression respectively among our respondents.

Results: Severe anxiety (68.8%) followed by moderate depression (60.5%) and moderate anger (56.9%) were the most common mental health challenges experienced by low-income earners in Bushenyi district. Awareness of mental healthcare increased with the age of respondents in both males and females. A linear relationship was observed with age and depression (r = 0.154, P = 0.014) while positive correlations were observed between anxiety and anger (r = 0.254, P < 0.001); anxiety and depression (r = 0.153, P = 0.015) and anger and depression (r = 0.153, P = 0.015).

Conclusion: The study shows the importance of mental health awareness in low resource settings during the current COVID-19 pandemic. Females were identified as persons at risk to mental depression, while anger was highest amongst young males.
Associations Between Physical Fitness, Objectively Measured Physical Activity and Academic Performance
Authors: Ðurić Saša , Bogataj Špela , Zovko Vinko , Sember Vedrana
Published: December 2021

There is evidence that physical activity (PA) can improve the academic performance. We recruited healthy adolescent girls, aged 11–12 years, and measured their PA with the accelerometer ActiGraph GT3X for the consecutive 5 days. Physical fitness (PF) was measured with eight motoric tests and three anthropometry measures. Academic performance (AP) was assessed for the six academic narrated school subjects. The results revealed that the girls were more physically active during the week days and less active at weekend (557 vs. 516 counts/min). Physical education grade shows the highest overall correlations with the results of the PF test battery (r= 0.53–0.95,p< 0.01). Nevertheless, correlations surprisingly decrease for the combined daily PA (r= 0.45), especially the weekend PA (r= 0.28). Grade point average and PF correlated moderately (r= 0.43-0.64), while they were moderate to high for PA (r= 0.59–0.87). Many questions arose after the completion of the present study and several new topics opened up, such as the question of how parental education affects the duration of PA and AP of the children and the influence of the place of residence AP of the children.
Geochemistry of Groundwater in the Uva Province, Sri Lanka—Implications for Chronic Kidney Disease of Uncertain Origin
Authors: Piyathilake I. D. U. H. , Udeshani W. A. C. , Hapuarachchi H. A. C. S. , Ranaweera L. V. , Udayakumara E. P. N. , Gunatilake S. K. , Dissanayake Chandra B.
Published: December 2021

The prevalence of chronic kidney disease of uncertain etiology (CKDu) in the Uva Province (UP) of Sri Lanka has received much attention over the past two decades. Many scientists assumed that prolonged consumption of drinking water with high levels of contaminants may be the causative factor. Thus, the prime objective of this study is to develop a binary logistic regression model based on water quality parameters and the prevalence of CKDu to find out the geochemical risk factors that affect the CKDu. For this, 260 groundwater samples were collected following a stratified random sampling method and analyzed for its major cations, anions, and selected trace element contents. In the model, the dichotomous dependent variable defines the availability of CKDu patients, and explanatory variables define groundwater quality parameters. According to the best-fit model, F− and PO43- levels of the groundwater were found to be the geochemical risk factors that were significantly associated with the progression of CKDu in the study area. Furthermore, it was shown that geochemical processes such as dissolution of bedrocks are the causative phenomenon of the enhancement of F− levels in the groundwater sources. It has also been observed that the PO43- concentrations of the groundwater possibly increase because of the intensive application of agrochemicals in addition to geogenic sources. The results of this study can be used by the government authorities in groundwater management and the management of the prevalence of CKDu disease in the study area. Furthermore, the findings of this study will contribute to the policy-makers in Sri Lanka for providing safe drinking water to meet the sustainable development goals (SDGs).
Finding Your Way: Shortest Paths on Networks
Authors: Rexin Teresa , Porter Mason A.
Published: December 2021

Traveling to different destinations is a major part of our lives. We visit a variety of locations both during our daily lives and when we are on vacation. How can we find the best way to navigate from one place to another? Perhaps we can test all of the different ways of traveling between two places, but another method is to use mathematics and computation to find a shortest path between them. In this article, we discuss how to construct shortest paths and introduce Dijkstra’s algorithm to minimize the total cost of a path, where the cost may be the travel distance, the travel time, or some other quantity. We also discuss how to use shortest paths in the real world to save time and increase traveling efficiency.
Potential Association Between the Low-Energy Plasma Structure and the Patchy Pulsating Aurora
Authors: Liang Jun , Nishimura Y. , Donovan E. , Yang B. , Angelopoulos V.
Published: December 2021

While the pulsating auroral phenomena have been recognized and studied for decades, our understating of their generation mechanisms remains incomplete to date. In one main class of pulsating auroras which is termed “patchy pulsating auroras” (PPA), the auroral patches are found to basically maintain their shape and size over many pulsation cycles. Also, PPAs are repeatedly found to essentially co-move with the ExB convection drift. The above properties led many researchers to hypothesize that PPA might connect to a structure of enhanced cold plasma in the magnetosphere. In this study, we review the existing evidence, and provide new perspective and support, of the low-energy plasma structure potentially associated with PPA. Based on observations from both the magnetosphere and the topside ionosphere, we suggest that ionospheric auroral outflows might constitute one possible source mechanism of the flux tubes with enhanced low-energy plasma that connect to the PPA. We also review the existing theories of pulsating auroras, with particular focus on the role of low-energy plasma in these theories. To date, none of the existing theories are complete and mature enough to offer a quantitatively satisfactory explanation of pulsating auroras. At last, we suggest a few future research directions to advance our understanding of pulsating auroras: a) more accurate measurements of the cold plasma density, b) more developed theories of the underlying mechanisms of ELF/VLF wave modulation, and c) auxiliary processes in the topside ionosphere or near-Earth region accompanying pulsating auroras.
The Interplay Between Affect, Dog's Physical Activity and Dog–Owner Relationship
Authors: Väätäjä Heli , Majaranta Päivi , Cardó Anna Valldeoriola , Isokoski Poika , Somppi Sanni , Vehkaoja Antti , Vainio Outi , Surakka Veikko
Published: December 2021

Leaving a dog home alone is part of everyday life for most dog owners. Previous research shows that dog–owner relationship has multifarious effects on dog behavior. However, little is known about the interplay between dog–owner relationship, physical activity of the dog, and affective experiences at the time of the owner leaving home and reunion when the owner comes home. In this paper, we explored how the general (daily, home alone, and over the 2-week study period) physical activity of the dog, and owner's perceptions of the dog's affective state were correlated at those particular moments. Nineteen volunteer dog owners had their dogs (N = 19) wear two activity trackers (ActiGraph wGT2X-GT and FitBark2) for 2 weeks 24 h/day. Prior to the 2-week continuous physical activity measurement period, the owners filled in questionnaires about the dog–owner relationship and the dog behavior. In daily questionnaires, owners described and assessed their own and their perception of the emotion-related experiences of their dog and behavior of the dog at the moment of separation and reunion. The results indicated that the dog–owner relationship has an interplay with the mean daily and weekly physical activity levels of the dog. An indication of strong emotional dog–owner relationship (especially related to the attentiveness of the dog, continuous companionship, and time spent together when relaxing) correlated positively with the mean daily activity levels of the dog during the first measurement week of the study. Results also suggest that the mean daily and over the 2-week measurement period physical activity of the dog correlated the affective experiences of the dog and owner as reported by the owner when the dog was left home alone. More research is needed to understand the interplay between affect, physical activity of the dog, dog–owner relationship, and the effects of these factors on, and their interplay with, the welfare of dogs.