bluuebunny/crossref_metadata_2025 huggingface dataset:
Rural Family Caregiving: A Closer Look at the Impacts of Health, Care Work, Financial Distress, and Social Loneliness on Anxiety
Authors: L’Heureux Tanya , Parmar Jasneet , Dobbs Bonnie , Charles Lesley , Tian Peter George J. , Sacrey Lori-Ann , Anderson Sharon
Published: June 2022

Even before the COVID-19 pandemic, earlier acute care patient discharges, restricted admissions to long-term care, and reduced home care services increased the amount and complexity of family caregivers’ care work. However, much less is known about rural caregivers’ experiences. Thus, our aim in this sequential mixed-methods study was to understand how COVID-19 affected rural family caregivers. Thematically analyzed interviews and linear regression on survey data were used to understand family caregiver stress. Fourteen rural caregivers participated in interviews. They acknowledged that they benefitted from the circle of support in rural communities; however, they all reported having to cope with fewer healthcare and social services. 126 rural caregivers participated in the online survey. About a third (31%) of these caregivers had moderate frailty, indicating that they could benefit from support to improve their health. In linear regression, frailty, social loneliness, financial hardship, and younger age were associated with caregiver anxiety. Contrary to the qualitative reports that people in rural communities are supportive, over two-thirds of the rural caregivers completing the survey were socially lonely. Rural family caregivers are vulnerable to anxiety and social loneliness due to the nature of caregiving and the lack of healthcare and social service supports in rural areas. Primary healthcare and home care teams are well-positioned to assess caregivers’ health and care situation as well as to signpost them to needed supports that are available in their areas.
Construct of the Association between Sleep Quality and Perinatal Depression: A Literature Review
Authors: Poeira Ana Filipa , Zangão Maria Otília
Published: June 2022

Pregnancy is characterized by hormonal and physiological changes; some of these changes cause changes in sleep, presenting excessive sleep in early pregnancy due to the action of progesterone, and difficulty sleeping at the end of pregnancy due to weight gain and frequency of urination. Objective: to identify and systematize the evidence on the association between sleep quality and perinatal depression in pregnant and postpartum women. Methods: an integrative literature review was carried out with a search in the CINAHL, MEDLINE, and SCOPUS databases using the PRISMA flowchart. Results: Of the 92 articles, 10 studies were included according to the eligibility criteria. Results indicate that poor sleep quality during pregnancy is predictive of prenatal and postnatal depression. Sleep quality worsens with increasing gestational and maternal age. Conclusions: Sleep quality during pregnancy is associated with perinatal depression, a global public health problem with high prevalence. Due to its severe consequences for women, children, and families, perinatal depression needs to be identified early, preferably during pregnancy or soon after childbirth, justifying the priority of screening and prevention.
Breast Cancer—How Can Imaging Help?
Authors: Iacob Roxana , Manolescu Diana Luminita , Stoicescu Emil Robert , Fabian Antonio , Malita Daniel , Oancea Cristian
Published: June 2022

Breast cancer is the most common malignant disease among women, causing death and suffering worldwide. It is known that, for the improvement of the survival rate and the psychological impact it has on patients, early detection is crucial. For this to happen, the imaging techniques should be used at their full potential. We selected and examined 44 articles that had as subject the use of a specific imaging method in breast cancer management (mammography, ultrasound, MRI, ultrasound-guided biopsy, PET-CT). After analyzing their data, we summarized and concluded which are the best ways to use each one of the mentioned techniques for a good outcome. We created a simplified algorithm with easy steps that can be followed by radiologists when facing this type of neoplasia.
Survey on Anxiety and Post-Traumatic Stress Disorder in Intensive Care Personnel during the COVID-19 Pandemic in a Medically Under-Resourced Country
Authors: Lazar Alexandra Elena , Szederjesi Janos , Coman Oana , Elekes Andrea , Suciaghi Mariana , Grigorescu Bianca Liana
Published: June 2022

The COVID-19 pandemic has increased the need for intensive care personnel. Romania has a low number of physicians per inhabitant. The stress of pandemics upon an already weak medical health system triggered some psychological effects upon burnt-out personnel. The main objective is to provide an insight into the psychological status of Romanian ICU personnel by evaluating their level of anxiety. The secondary objectives aim to identify the level of post-traumatic stress disorder and anxiety in different groups and to identify the personnel most affected psychologically. This study enrolled adult responders from the ICU of Târgu Mureș Emergency Clinical County Hospital, Romania, participating voluntarily. The evaluation tests were the State-Trait Anxiety Inventory and Post-Traumatic Stress Test. Out of the 126 eligible participants, 87 adult employees were enrolled—with a 69% response rate. The study comprised three groups: doctors, nurses, and auxiliary personnel. All three groups scored for moderate anxiety symptoms. COVID-19-related anxiety was strongly correlated with age and number of working years in all groups. Increased PTSD scores were observed in doctors and nurses. All ICU personnel who dealt with COVID-19 patients presented with moderate anxiety and post-traumatic stress disorder symptoms. The years of ICU experience had a positive impact on anxiety symptoms.
Analyses of Stalked Jellyfish in Kitsunezaki, Japan: Calvadosia nagatensis, and Two Lineages of Haliclystus inabai with Early Life Stages Observed in an Aquarium in Canada
Authors: Adriansyah Amanda S. , Astri Agatha , Hirano Yayoi , Collins Allen G. , Deshaies Marie-Lyne , Putra Delta , Sekiguchi Shu , Ikeda Shuhei , Okuizumi Kazuya , Chikuchishin Mitsuko , Aoki Masakazu , Ames Cheryl L.
Published: June 2022

In this work, staurozoans of two distinct morphotypes are reported in Kitsunezaki (Ishinomaki City, Miyagi, Japan) in the years following the Great East Japan Earthquake and Tsunami. Staurozoa specimens were collected from Eisenia and Gelidium macroalgal beds at the Kitsunezaki survey site (October 2019–July 2021). Morphological observations indicated that the Kitsunezaki staurozoans represented two species, Haliclystus inabai and Calvadosia nagatensis, but molecular analyses of the genetic markers 16S rRNA and COI suggested that the former actually encompasses two distinct lineages, H. inabai and a cryptic as yet unnamed species. Phylogenetic analysis reveals the two H. inabai lineages are separated by significant divergences for both gene markers. H. inabai lineage 1 includes specimens sampled with molecular sequences from Hokkaido (Japan) and Kitsunezaki (Japan), whereas H. inabai lineage 2 includes sequences from Victoria (Australia), Kitsunezaki, as well as populations that appeared in a lab in Germany and aquariums in Tsuruoka and Kagoshima (Japan) and Québec (Canada). Conversely, C. nagatensis from Kitsunezaki appears to be a species distributed only in the temperate NW Pacific. Observations on early life stages of H. inabai lineage 2 within aquarium tanks permitted confirmation of the presence of “microhydrula” settled larva, frustules, and elongated settled larvae. C. nagatensis was collected from the Kitsunezaki survey site in warm months only, and always exhibited gonads, while H. inabai stauromedusae were collected in most months throughout the year, with gonads usually present irrespective of season. An extensive literature review covering more than 100 years and observations in this study revealed seaweed and seagrass as the primary substrates for these two Staurozoa species. Our findings show C. nagatensis is associated with just two types of algal substrates and seagrass, while H. inabai has a much broader substrate preference, consistent with its wider geographic distribution. These findings have contributed to our understanding of Staurozoa epibiotic associations in exposed bays during the recovery period following a major natural disaster.
Emergence of the G118R Pan-Integrase Resistance Mutation as a Result of Low Compliance to a Dolutegravir-Based cART
Authors: Mens Helene , Fjordside Lasse , Fonager Jannik , Gerstoft Jan
Published: June 2022

HIV-1 resistance towards integrase inhibitors is a potential threat of the success of HIV-1 combination treatment. G118R is a rare drug resistance mutation conferring pan-integrase resistance. Here, we describe the occurrence of G118R in a HIV-1 subtype-B-positive individual with major compliance problems, detected while the patient was on dolutegravir-based cART. We speculate the pre-selection of M184I/V aided the occurrence of G118R in this case, and discuss the robustness of dolutegravir-based therapies.
Construct Validity and Responsiveness of Instruments Measuring Depression and Anxiety in Pregnancy: A Comparison of EPDS, HADS-A and CES-D
Authors: Heller Hanna Margaretha , Draisma Stasja , Honig Adriaan
Published: June 2022

Depression and anxiety occur frequently in pregnancy and may have unfavourable consequences for mother and child. Therefore, adequate symptom measurement seems important. Commonly used instruments are the Center for Epidemiologic Studies Depression Scale (CES-D), the Edinburgh Postpartum Depression Scale (EPDS), and the Hospital Anxiety and Depression Scale, anxiety subscale (HADS-A). We compared the (1) structural and (2) longitudinal validity of these instruments. The data originated from a study on the effectiveness of an Internet intervention for pregnant women with affective symptoms. (1) A confirmatory factor analysis was used to estimate the construct validity. The theoretical factorial structure that was defined in earlier studies of the CES-D and the EPDS, but not the HADS-A, could be sufficiently replicated with acceptable CFI and RMSEA values. (2) Since there were two measurements in time, the hypotheses concerning plausible directions of the change scores of subscales that were (un)related to each other could be formulated and tested. In this way, longitudinal validity in the form of responsiveness was estimated. Ten of sixteen hypotheses were confirmed, corroborating the longitudinal validity of all constructs, except anhedonia, probably due to inconsistent conceptualization. The HADS-A seems less suitable to screen for anxiety in pregnancy. Anhedonia needs better conceptualisation to assess the change of symptoms over time with the CES-D and the EPDS.
Conservative Media Use and COVID-19 Related Behavior: The Moderating Role of Media Literacy Variables
Authors: Borah Porismita , Lorenzano Kyle , Vishnevskaya Anastasia , Austin Erica
Published: June 2022

At the start of the COVID-19 pandemic, there was no vaccine to cure or slow its impact due to the novelty of the virus, nor were there were any other standardized measures to handle its spread. Yet, despite the detrimental consequences of the pandemic and its impact on people’s lives, the behavior of individuals to combat the pandemic was not necessarily consistent with official guidelines. To make things worse, the pandemic was highly politicized in countries such as the U.S. With a help of a national survey from the U.S., we examine the associations between media literacy variables and willingness to perform recommended COVID-19 related health behavior. Moreover, we also examine the moderating role of conservative media use in this relationship. Our findings show that conservative media use was negatively associated with these protective behaviors, and that both media literacy variables were positively related with willingness to perform recommended COVID-19 related health behavior. Our results show that media literacy can mitigate some of the impact of conservative media use on individuals. Our findings help understand the complexity of protective behavior against the virus during a highly politicized pandemic.
COVID-19 Victimization Experience and College Students’ Mobile Phone Addiction: A Moderated Mediation Effect of Future Anxiety and Mindfulness
Authors: Chen Lili , Li Jun , Huang Jianhao
Published: June 2022

This study proposed a moderated mediation model to investigate the association between COVID-19 victimization experience and mobile phone addiction, the mediating role of future anxiety, and the moderating role of mindfulness. This study employed the COVID-19 victimization experience scale, the mobile phone addiction scale, a future anxiety scale, and a mindfulness scale in a survey study among Chinese college students; 840 valid questionnaires were received. The reliability and confirmatory factor analysis results showed that all four scales had good reliability and validity. Bootstrap results demonstrated that COVID-19 victimization experience significantly predicted mobile phone addiction in college students (B = 0.202, LLCI = 0.136, ULCI = 0.268). Future anxiety fully mediated the association between COVID-19 victimization experience and mobile phone addiction (B = 0.178, LLCI = 0.136, ULCI = 0.222). Mindfulness moderated the effect of COVID-19 victimization experience on the college students’ future anxiety (B = 0.159, LLCI = 0.007, ULCI = 0.054). A higher level of mindfulness was more likely than a lower level of mindfulness to attenuate the effect of COVID-19 victimization experience on the college students’ future anxiety. These findings broaden our understanding regarding the association between COVID-19 victimization experience and mobile phone addiction and the moderating role of mindfulness.
Determination of Critical Power Using Different Possible Approaches among Endurance Athletes: A Review
Authors: Lipková Lucie , Kumstát Michal , Struhár Ivan
Published: June 2022

Critical power represents an important parameter of aerobic function and is the highest average effort that can be sustained for a period of time without fatigue. Critical power is determined mainly in the laboratory. Many different approaches have been applied in testing methods, and it is a difficult task to determine which testing protocol it the most suitable. This review aims to evaluate all possible tests on bicycle ergometers or bicycles used to estimate critical power and to compare them. A literature search was conducted in four databases (PubMed, Scopus, SPORTDiscus, and Web of Science) published from 2012 to 2022 and followed the PRISMA guidelines to process the review. Twenty-one articles met the eligibility criteria: records with trained or experienced endurance athletes (adults > 18), bicycle ergometer, a description of the testing protocol, and comparison of the tests. We found that the most widely used tests were the 3-min all-out tests set in a linear mode and the traditional protocol time to exhaustion. Some other alternatives could have been used but were not as regular. To summarize, the testing methods offered two main approaches in the laboratory (time to exhaustion test andthe 3-min all-out test with different protocols) and approach in the field, which is not yet completely standardized.
Health Communication through Chinese Media on E-Cigarette: A Topic Modeling Approach
Authors: Liu Qian , Liang Yu , Wang Siyi , Huang Zhongguo , Wang Qing , Jia Miaoyutian , Li Zihang , Ming Wai-Kit
Published: June 2022

Background: Electronic cigarettes (e-cigarettes) have been a newsworthy topic in China. E-cigarettes are receiving greater consumer attention due to the rise of the Chinese e-cigarettes industry. In the past decade, e-cigarettes have been widely debated across the media, particularly their identity and their health effects. Objective: this study aims to (1) find the key topics in e-cigarette news and (2) provide suggestions for future media strategies to improve health communication. Method: We collected Chinese e-cigarettes news from 1 November 2015 to 31 October 2020, in the Huike (WiseSearch) database, using “e-cigarettes” (Chinese: “电子烟”) as the keyword. We used the Jieba package in python to perform the data cleaning process and the Dirichlet allocation (LDA) topic modeling method to generate major themes of the health communication through news content. Main finding: through an analysis of 1584 news articles on e-cigarettes, this paper finds 26 topics covered with 4 themes as regulations and control (n = 475, 30%), minor protection (n = 436, 27.5%), industry activities (n = 404, 25.5%), and health effects (n = 269, 17%). The peak and decline of the number of news articles are affected by time and related regulations. Conclusion: the main themes of Chinese news content on e-cigarettes are regulations and control, and minor protection. Newspapers should shoulder the responsibilities and play an important role in health communication with balanced coverage.
Sleep Problems and 6-Sulfatoxymelatonin as Possible Predictors of Symptom Severity, Adaptive and Maladaptive Behavior in Children with Autism Spectrum Disorder
Authors: Bartakovicova Kristina , Kemenyova Petra , Belica Ivan , Janik Szapuova Zofia , Stebelova Katarina , Waczulikova Iveta , Ostatnikova Daniela , Babinska Katarina
Published: June 2022

In children with autism spectrum disorder (ASD), sleep disturbances are a frequent comorbidity with an adverse effect on their behavior and functioning. It was suggested that melatonin deficit is at least partly responsible for the sleep problems. The study aimed to investigate, in a sample of 56 children with ASD aged 2.8–13.3 years, if the sleep problems and melatonin secretion can serve as predictors of adaptive functioning and severity of the ASD core symptoms. We demonstrated that, after adjustment for age, the Sleep score assessed by the Children’s Sleep Habits Questionnaire predicts the Adaptive behavior composite score only in children younger than 6 years, and the preferred predictive model is for the domain Socialization. The age-adjusted Sleep score predicted Externalizing and Internalizing maladaptive behavior, with a near-zero contribution of age to the relationship between the Internalizing maladaptive behavior and Sleep score. After adjustment for age, the reduced night-time melatonin secretion predicted a higher severity of ASD symptoms in the domain Social affect and the Calibrated Severity Score, but not the sleep problems. Our results emphasize the importance of assessing sleep problems as a modifiable predictor of behavior in children with ASD and support the hypothesis about the role of melatonin in pathophysiology of ASD.
Evaluation of Measurement Procedures for Solid Particle Number (SPN) Measurements during the Periodic Technical Inspection (PTI) of Vehicles
Authors: Melas Anastasios , Selleri Tommaso , Suarez-Bertoa Ricardo , Giechaskiel Barouch
Published: June 2022

Periodic technical inspection (PTI) of vehicles guarantees safety and environmental compliance during their lifetime. Particulate matter emissions of diesel vehicles are controlled with opacity measurements. After the introduction of diesel particulate filters (DPFs), particulate matter emissions have drastically decreased and the sensitivity of the opacity method is questioned. Several countries have already or are planning to introduce a solid particle number (SPN) method at their PTI that will either substitute or complement opacity measurements. However, there are differences in the measurement procedures and the limit values. In this study, we compared the different approaches and investigated topics which are still not well defined, such as the uncertainty of the SPN-PTI instruments, repeatability of the procedures, impact of the DPF fill state, and the correlation between type-approval SPN emissions and SPN concentrations during PTI tests. Finally, we compared the SPN-PTI instruments with the opacity meters. Our results showed that SPN-PTI measurements can detect tampered and defective DPFs. We also made suggestions on the measurement procedures and the concentration limit.
Is Teledentistry a Method for Optimizing Dental Practice, Even in the Post-Pandemic Period? An Integrative Review
Authors: Kui Andreea , Popescu Codruța , Labuneț Anca , Almășan Oana , Petruțiu Adrian , Păcurar Mariana , Buduru Smaranda
Published: June 2022

Background: For the past two and half years, dentists all across the world, along with their patients, have faced numerous challenges. In this context, the aim of this integrative review was to assess if dentists’ and patients’ attitudes regarding teledentistry (TD) have changed since the COVID-19 outbreak, and if the use of TD will continue to rise, even in the post-pandemic period; (2) Methods: A literature search was performed between August 2021 and January 2022. The PubMed, Scopus, and Science Direct databases were searched for articles published between 2012 and 2022 using a combination of the following Mesh terms: “COVID-19”, “pandemic”, “oral telemedicine”, “teledentistry”; (3) Results: Among the 52 included papers, nine papers were published between 2011 and 2019, and 43 articles were published after 2020 (12 were published in 2020, 29 papers were published in 2021, and two in 2022). Among the articles published before 2020, seven out of nine included papers were reviews, and two were original research. Among the 43 papers published after 2020, 18 were reviews and 25 original research. (4) Conclusions: Based on the results of this integrative review, there is clear evidence that the interest in teledentistry and teleassistance in the dental field has increased rapidly, especially in the context of the COVID-19 pandemic. Therefore, while dental practitioners should be encouraged to keep themselves updated about new technologies, patients should also be constantly informed about their options for receiving special oral health care.
Spatial-Temporal Attentive LSTM for Vehicle-Trajectory Prediction
Authors: Jiang Rui , Xu Hongyun , Gong Gelian , Kuang Yong , Liu Zhikang
Published: June 2022

Vehicle-trajectory prediction is essential for intelligent traffic systems (ITS), as it can help autonomous vehicles to plan a safe and efficient path. However, it is still a challenging task because existing studies have mainly focused on the spatial interactions of adjacent vehicles regardless of the temporal dependencies. In this paper, we propose a spatial-temporal attentive LSTM encoder–decoder model (STAM-LSTM) to predict vehicle trajectories. Specifically, the spatial attention mechanism is used to capture the spatial relationships among neighboring vehicles and then obtain the global spatial feature. Meanwhile, the temporal attention mechanism is designed to distinguish the effects of different historical time steps on future trajectory prediction. In addition, the motion feature of vehicles is extracted to reveal the influence of dynamic information on vehicle-trajectory prediction, and is combined with the local and global spatial features to represent the integrated features of the target vehicle at each historical moment. The experiments were conducted on public highway trajectory datasets—US-101 and I-80 in NGSIM—and the results demonstrate that our model achieves state-of-the-art prediction performance.
In Silico Molecular Dynamics of Griseofulvin and Its Derivatives Revealed Potential Therapeutic Applications for COVID-19
Authors: Aris Parisa , Mohamadzadeh Masoud , Wei Yulong , Xia Xuhua
Published: June 2022

Treatment options for Coronavirus Disease 2019 (COVID-19) remain limited, and the option of repurposing approved drugs with promising medicinal properties is of increasing interest in therapeutic approaches to COVID-19. Using computational approaches, we examined griseofulvin and its derivatives against four key anti-SARS-CoV-2 targets: main protease, RdRp, spike protein receptor-binding domain (RBD), and human host angiotensin-converting enzyme 2 (ACE2). Molecular docking analysis revealed that griseofulvin (CID 441140) has the highest docking score (–6.8 kcal/mol) with main protease of SARS-CoV-2. Moreover, griseofulvin derivative M9 (CID 144564153) proved the most potent inhibitor with −9.49 kcal/mol, followed by A3 (CID 46844082) with −8.44 kcal/mol against M protease and ACE2, respectively. Additionally, H bond analysis revealed that compound A3 formed the highest number of hydrogen bonds, indicating the strongest inhibitory efficacy against ACE2. Further, molecular dynamics (MD) simulation analysis revealed that griseofulvin and these derivatives are structurally stable. These findings suggest that griseofulvin and its derivatives may be considered when designing future therapeutic options for SARS-CoV-2 infection.
The Role of Nitric Oxide Signaling in Plant Responses to Cadmium Stress
Authors: Meng Yuting , Jing Huaikang , Huang Jing , Shen Renfang , Zhu Xiaofang
Published: June 2022

Nitric oxide (NO) is a widely distributed gaseous signaling molecule in plants that can be synthesized through enzymatic and non-enzymatic pathways and plays an important role in plant growth and development, signal transduction, and response to biotic and abiotic stresses. Cadmium (Cd) is a heavy metal pollutant widely found in the environment, which not only inhibits plant growth but also enters humans through the food chain and endangers human health. To reduce or avoid the adverse effects of Cd stress, plants have evolved a range of coping mechanisms. Many studies have shown that NO is also involved in the plant response to Cd stress and plays an important role in regulating the resistance of plants to Cd stress. However, until now, the mechanisms by which Cd stress regulates the level of endogenous NO accumulation in plant cells remained unclear, and the role of exogenous NO in plant responses to Cd stress is controversial. This review describes the pathways of NO production in plants, the changes in endogenous NO levels in plants under Cd stress, and the effects of exogenous NO on regulating plant resistance to Cd stress.
Non-Covalent Interaction on the Self-Healing of Mechanical Properties in Supramolecular Polymers
Authors: Buaksuntear Kwanchai , Limarun Phakamat , Suethao Supitta , Smitthipong Wirasak
Published: June 2022

Supramolecular polymers are widely utilized and applied in self-assembly or self-healing materials, which can be repaired when damaged. Normally, the healing process is classified into two types, including extrinsic and intrinsic self-healable materials. Therefore, the aim of this work is to review the intrinsic self-healing strategy based on supramolecular interaction or non-covalent interaction and molecular recognition to obtain the improvement of mechanical properties. In this review, we introduce the main background of non-covalent interaction, which consists of the metal–ligand coordination, hydrogen bonding, π–π interaction, electrostatic interaction, dipole–dipole interaction, and host–guest interactions, respectively. From the perspective of mechanical properties, these interactions act as transient crosslinking points to both prevent and repair the broken polymer chains. For material utilization in terms of self-healing products, this knowledge can be applied and developed to increase the lifetime of the products, causing rapid healing and reducing accidents and maintenance costs. Therefore, the self-healing materials using supramolecular polymers or non-covalent interaction provides a novel strategy to enhance the mechanical properties of materials causing the extended cycling lifetime of products before replacement with a new one.
Identification and Characterization of Abiotic Stress–Responsive NF-YB Family Genes in Medicago
Authors: Du Wenxuan , Yang Junfeng , Li Qian , He Chunfeng , Pang Yongzhen
Published: June 2022

Nuclear factor YB (NF-YB) are plant-specific transcription factors that play a critical regulatory role in plant growth and development as well as in plant resistance against various stresses. In this study, a total of 49 NF-YB genes were identified from the genomes of Medicago truncatula and Medicago sativa. Multiple sequence alignment analysis showed that all of these NF-YB members contain DNA binding domain, NF-YA interaction domain and NF-YC interaction domain. Phylogenetic analysis suggested that these NF-YB proteins could be classified into five distinct clusters. We also analyzed the exon–intron organizations and conserved motifs of these NF-YB genes and their deduced proteins. We also found many stress-related cis-acting elements in their promoter region. In addition, analyses on genechip for M. truncatula and transcriptome data for M. sativa indicated that these NF-YB genes exhibited a distinct expression pattern in various tissues; many of these could be induced by drought and/or salt treatments. In particular, RT-qPCR analysis revealed that the expression levels of gene pairs MsNF-YB27/MtNF-YB15 and MsNF-YB28/MtNF-YB16 were significantly up-regulated under NaCl and mannitol treatments, indicating that they are most likely involved in salt and drought stress response. Taken together, our study on NF-YB family genes in Medicago is valuable for their functional characterization, as well as for the application of NF-YB genes in genetic breeding for high-yield and high-resistance alfalfa.
Salivary Effector Sm9723 of Grain Aphid Sitobion miscanthi Suppresses Plant Defense and Is Essential for Aphid Survival on Wheat
Authors: Zhang Yong , Liu Xiaobei , Fu Yu , Crespo-Herrera Leonardo , Liu Huan , Wang Qian , Zhang Yumeng , Chen Julian
Published: June 2022

Aphid salivary effectors play important roles in modulating plant defense responses. The grain aphid Sitobion miscanthi is one of the most economically important cereal aphids worldwide. However, little information is available on the identification and functional analysis of salivary effectors of S. miscanthi. In this study, a candidate salivary effector Sm9723 was identified, which was specifically expressed in aphid salivary glands and highly induced during the aphid feeding phase. Transient overexpression of Sm9723 in Nicotiana benthamiana suppressed BAX and INF1-induced cell death. Further, Sm9723 overexpression inhibited N. benthamiana defense responses by reducing pattern-triggered immunity associated callose deposition and expression levels of jasmonic and salicylic acid-associated defense genes. In addition, the salivary effector Sm9723 of S. miscanthi was effectively silenced through nanocarrier-mediated dsRNA delivery system. After silencing Sm9723, fecundity and survival of S. miscanthi decreased significantly, and the aphid feeding behavior was also negatively affected. These results suggest salivary effector Sm9723 is involved in suppressing plant immunity and is essential in enabling aphid virulence, which could be applied as potential target gene for RNAi-mediated pest control of S. miscanthi.
Importance of Matrix Cues on Intervertebral Disc Development, Degeneration, and Regeneration
Authors: Kibble Matthew J. , Domingos Marco , Hoyland Judith A. , Richardson Stephen M.
Published: June 2022

Back pain is one of the leading causes of disability worldwide and is frequently caused by degeneration of the intervertebral discs. The discs’ development, homeostasis, and degeneration are driven by a complex series of biochemical and physical extracellular matrix cues produced by and transmitted to native cells. Thus, understanding the roles of different cues is essential for designing effective cellular and regenerative therapies. Omics technologies have helped identify many new matrix cues; however, comparatively few matrix molecules have thus far been incorporated into tissue engineered models. These include collagen type I and type II, laminins, glycosaminoglycans, and their biomimetic analogues. Modern biofabrication techniques, such as 3D bioprinting, are also enabling the spatial patterning of matrix molecules and growth factors to direct regional effects. These techniques should now be applied to biochemically, physically, and structurally relevant disc models incorporating disc and stem cells to investigate the drivers of healthy cell phenotype and differentiation. Such research will inform the development of efficacious regenerative therapies and improved clinical outcomes.
Correction: Cutuli et al. Neuroprotective Role of Dietary Supplementation with Omega-3 Fatty Acids in the Presence of Basal Forebrain Cholinergic Neurons Degeneration in Aged Mice. Int. J. Mol. Sci. 2020, 21, 1741
Authors: Cutuli Debora , Landolfo Eugenia , Decandia Davide , Nobili Annalisa , Viscomi Maria Teresa , La Barbera Livia , Sacchetti Stefano , De Bartolo Paola , Curci Annacarmen , D’Amelio Marcello , Farioli-Vecchioli Stefano , Petrosini Laura
Published: June 2022

The authors wish to make the following corrections to this paper [...]
The Role of Inflammatory Mediators in the Development of Gastrointestinal Motility Disorders
Authors: Docsa Tibor , Sipos Adám , Cox Charles S. , Uray Karen
Published: June 2022

Feeding intolerance and the development of ileus is a common complication affecting critically ill, surgical, and trauma patients, resulting in prolonged intensive care unit and hospital stays, increased infectious complications, a higher rate of hospital readmission, and higher medical care costs. Medical treatment for ileus is ineffective and many of the available prokinetic drugs have serious side effects that limit their use. Despite the large number of patients affected and the consequences of ileus, little progress has been made in identifying new drug targets for the treatment of ileus. Inflammatory mediators play a critical role in the development of ileus, but surprisingly little is known about the direct effects of inflammatory mediators on cells of the gastrointestinal tract, and many of the studies are conflicting. Understanding the effects of inflammatory cytokines/chemokines on the development of ileus will facilitate the early identification of patients who will develop ileus and the identification of new drug targets to treat ileus. Thus, herein, we review the published literature concerning the effects of inflammatory mediators on gastrointestinal motility.
A Preliminary Study on Photic Driving in the Electroencephalogram of Children with Autism across a Wide Cognitive and Behavioral Range
Authors: Vetri Luigi , Maniscalco Laura , Diana Paola , Guidotti Marco , Matranga Domenica , Bonnet-Brilhault Frédérique , Tripi Gabriele
Published: June 2022

Intermittent photic stimulation (IPS) is a useful technique in electroencephalography (EEG) to investigate the neurophysiological anomalies of brain activity. Although not an active task, IPS has also been explored in ASD; it is thought to capture local potential oscillators at specific frequencies and perhaps tap into rhythmic activity in a way that general resting-state recordings cannot. Previous studies suggest that individuals with ASD showed photic driving reactivity predominantly at lower frequencies of stimulation. In our study we used IPS to measure rhythmic oscillatory activity in a sample of 81 ASD children. We found a significant correlation linking ASD children with photic driving activation only at low frequencies (δθ band) and increased severity of “restricted behavior”. This suggests that ASD children with higher severity of restricted behaviors could have a hypersynchronous θ power and an impaired resonance synchronization at middle-ranged frequencies (α). Furthermore, we found some evidence of hemispherical oscillatory asymmetry linked particularly to behavioral impairments. This result is in line with the EEG pattern model indicating a “U-shaped profile” of electrophysiological power alterations with excess power in low- and high-frequency bands and a reduction of power in the middle-ranged frequencies. IPS technique in electroencephalography is confirmed to reveal EEG biomarkers in autistic children, with a focus on spectral power, coherence, and hemisphere asymmetries.
Lethal Congenital Contracture Syndrome 11: A Case Report and Literature Review
Authors: Potrony Miriam , Borrell Antoni , Masoller Narcís , Nadal Alfons , Rodriguez-Carunchio Leonardo , Saez de Gordoa Elizalde Karmele , Quesada-Espinosa Juan Francisco , Villanueva-Cañas Jose Luis , Pauta Montse , Jodar Meritxell , Madrigal Irene , Badenas Celia , Alvarez-Mora Maria Isabel , Rodriguez-Revenga Laia
Published: June 2022

Lethal congenital contracture syndrome 11 (LCCS11) is caused by homozygous or compound heterozygous variants in the GLDN gene on chromosome 15q21. GLDN encodes gliomedin, a protein required for the formation of the nodes of Ranvier and development of the human peripheral nervous system. We report a fetus with ultrasound alterations detected at 28 weeks of gestation. The fetus exhibited hydrops, short long bones, fixed limb joints, absent fetal movements, and polyhydramnios. The pregnancy was terminated and postmortem studies confirmed the prenatal findings: distal arthrogryposis, fetal growth restriction, pulmonary hypoplasia, and retrognathia. The fetus had a normal chromosomal microarray analysis. Exome sequencing revealed two novel compound heterozygous variants in the GLDN associated with LCCS11. This manuscript reports this case and performs a literature review of all published LCCS11 cases.
Incidence and Risk Factors for Berger’s Space Development after Uneventful Cataract Surgery: Evidence from Swept-Source Optical Coherence Tomography
Authors: Zhang Zhengwei , Yao Jinhan , Chang Shuimiao , Kanclerz Piotr , Khoramnia Ramin , Deng Minghui , Wang Xiaogang
Published: June 2022

Background: This study investigates the incidence and risk factors for the development of Berger’s space (BS) after uneventful phacoemulsification based on swept-source optical coherence tomography (SS-OCT). Methods: Cataractous eyes captured using qualified SS-OCT images before and after uneventful phacoemulsification cataract surgery were included. Six high-resolution cross-sectional anterior segment SS-OCT images at 30° intervals were used for BS data measurements. BS width was measured at three points on each scanned meridian line: the central point line aligned with the cornea vertex and two point lines at the pupil’s margins. Results: A total of 223 eyes that underwent uneventful cataract surgery were evaluated. Preoperatively, only two eyes (2/223, 0.9%) were observed to have consistent BS in all six scanning directions. BS was observed postoperatively in 44 eyes (44/223, 19.7%). A total of 13 eyes (13/223, 5.8%) with insufficient image quality, pupil dilation, or lack of preoperative image data were excluded from the study. A total of 31 postoperative eyes with BS and 31 matched eyes without BS were included in the final data analysis. The smallest postoperative BS width was in the upper quadrant of the vertical meridian line (90°), with a mean value of 280 μm. The largest BS width was observed in the opposite area of the main clear corneal incision, with a mean value >500 μm. Conclusions: Uneven-width BS is observable after uneventful phacoemulsification. Locations with a much wider BS (indirect manifestation of Wieger zonular detachment) are predominantly located in the opposite direction to the main corneal incisions.
New Practice of E-Commerce Platform: Evidence from Two Trade-In Programs
Authors: Hu Qiang , Lou Tingyuan , Li Jicai , Zuo Wenjin , Chen Xihui , Ma Lindong
Published: June 2022

In the context of developing the digital platform economy, trade-in programs have become an effective strategy for e-commerce platforms to stimulate consumption. Many head e-commerce platforms have launched their own trade-in programs. However, the existing research on trade-in programs is still stuck in the traditional trade-in model. The purpose of this study is to explore whether there is a new and more beneficial trade-in program. In this paper, we construct the Stackelberg game model between a brand owner and a B2C e-commerce platform under two trade-in programs and use optimization theory to obtain the equilibrium results of the model. The results indicate that the performance improvement of the new-generation product will promote the increase in two-generation products’ price under traditional trade-in programs, the price of the new-generation product will increase, and the price of the previous-generation product will decrease under new trade-in programs. The brand owner always prefers traditional trade-in to new trade-in. However, the e-commerce platform prefers traditional trade-in to new trade-in just when the previous-generation product is durable enough and the performance improvement of a new-generation product is small enough; otherwise, it prefers new trade-in to traditional trade-in. These findings are beneficial to the operational practices of e-commerce platforms and brand owners.
Mining Heritage and Mining Landscape Krušnohoří/Erzgebirge as a Part of the UNESCO Heritage
Authors: Jelen Jakub
Published: June 2022

The article deals with the perception and methods of management of the mining heritage and mining the landscapes from the perspective of individual stakeholders, entities and interest groups. The first part deals with the conceptualization of heritage in general, discussing various ways of defining and looking at heritage; later, the general discussion focuses on a specific group of heritage, which is the mining heritage. The following is an introduction to the area of interest of the Jáchymov region (part of the Ore Mountains, i.e., an area characterized by a long mining history and a number of mining monuments). It is important, mainly, because it is part of the world heritage; the Mining Cultural Landscape of the Krušnohoří/Erzgebirge was added to the UNESCO list in 2019. The second part of the article is devoted to field research in the Jáchymov region. The aim of the research was to find out how the mining heritage in the Jáchymov region is treated, and how and for what purposes it is used. Representatives of the public, private and non-profit sectors from the local, regional and national levels were involved in the research. Research is a comprehensive view of the process of heritage formation and management.
Service of General Interest in the Rhenish Coal-Mining Area in Context of Structural Change
Authors: Gall-Roehrig Saskia , Rosier Steffen , Riss Katharina , Greiving Stefan
Published: June 2022

Territorial cohesion is of main interest in the EU and its member state Germany. It should lead to a reduction of disparities between different stats of development and is closely related to the accessibility to Service of General (SGI) in different areas. The investigated area in this article is characterized by the end of coal fired power generation by the Coal-Phase-out act. In this article the accessibility to SGI is discussed. Additionally, the accessibility to specific SGI is investigated with a special view to the southern rural parts of the Rhenish coal-mining area. It is outlined that the provision to SGIs is high near to agglomerations and decreases in rural parts. This can also be observed for broadband expansion, while the access to internet allows a higher accessibility to SGIs for different analysed indicators. This research is used to establish a regional development strategy to maintain equal living conditions for the entire region.
Climate Impact Assessment for Sustainable Structural Change in the Rhenish Lignite Mining Region
Authors: Becker Dennis , Othmer Felix J. , Greiving Stefan
Published: June 2022

The Rhenish lignite mining region is facing enormous structural changes due to the withdrawal from opencast mining. The current planning of the regional transformation process, however, has so far only insufficiently considered the local impacts of climate change and thus also existing needs for action as well as opportunities in the context of sustainable regional development. However, the integrated consideration of these challenges is probably one of the biggest strategic challenges for this region. The aim of our study was therefore to apply a method for the integrated analysis of structural and climatic changes in the context of a climate impact assessment. We follow a parallel spatial modelling approach and use scenario corridors to describe the bandwidth of potential future conditions. The results clearly show the influence of other drivers such as changes in population and land use, and thus the adaptation options within the context of a sustainable transformation process. Structural changes should be considered in the context of climate impact analyses, as well as climate changes in the management of structural change.
Optimizing Crowdsourced Land Use and Land Cover Data Collection: A Two-Stage Approach
Authors: Moltchanova Elena , Lesiv Myroslava , See Linda , Mugford Julie , Fritz Steffen
Published: June 2022

Citizen science has become an increasingly popular approach to scientific data collection, where classification tasks involving visual interpretation of images is one prominent area of application, e.g., to support the production of land cover and land-use maps. Achieving a minimum accuracy in these classification tasks at a minimum cost is the subject of this study. A Bayesian approach provides an intuitive and reasonably straightforward solution to achieve this objective. However, its application requires additional information, such as the relative frequency of the classes and the accuracy of each user. While the former is often available, the latter requires additional data collection. In this paper, we present a two-stage approach to gathering this additional information. We demonstrate its application using a hypothetical two-class example and then apply it to an actual crowdsourced dataset with five classes, which was taken from a previous Geo-Wiki crowdsourcing campaign on identifying the size of agricultural fields from very high-resolution satellite imagery. We also attach the R code for the implementation of the newly presented approach.
Effect of Waste Basalt Fines and Recycled Concrete Components on Mechanical, Water Absorption, and Microstructure Characteristics of Concrete
Authors: Sharaky Ibrahim A. , Elamary Ahmed S. , Alharthi Yasir M.
Published: June 2022

In this paper, the recycled fine aggregates and powders produced from crushing old basaltic concrete and natural basalt were used to produce new concrete. The sand was partially replaced by two types of recycled wastes at five percentages: 0%, 20%, 40%, 60%, and 80%. The cement was partially replaced by recycled powders and silica fume (SF) at four percentages: 0, 5%, 10%, and 20%. The concrete strengths and water absorption were obtained at several curing ages. The obtained results emphasized the positive effects of increasing the curing time on enhancing the concrete properties, regardless of the types or the waste sources. Moreover, the recycled powders retarded the hydration reaction. In addition, the recycled fine aggregates and powders could achieve about 99.5% and 99.3% of the ordinary concrete strength and enhance the tensile strength. Furthermore, the mix containing 40% of recycled fine concrete aggregate diffused the highest contents of both calcium and silicate, which led to enhancing the interfacial transition zone (ITZ) and concrete properties, compared to the other tested mixes. Finally, the water absorption of all tested concrete mixes decreased with an increase in the curing age, while the mixes integrating 10% and 20% of SF experienced the lowest values of water absorption.