bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of DNA Barcoding to Assess Phylogenetic β-Diversity in Mid-Subtropical Evergreen Broad-Leaved Forests of China
Authors: Liu Juan , Liu Jiajia , Ge Xuejun , Huang Guomin , Zhou Zengliang , Wan Songze
Published: October 2019

The application of quantifying phylogenetic information into measures of forest β-diversity is increasing for investigating the underlying drivers of community assembly along environmental gradients. In terms of assessing evolutionary inferences of community processes, a variety of plant DNA barcodes has been widely used in phylogenetic diversity measurements. However, relatively few studies have evaluated the effectiveness of DNA barcodes with using nuclear region in estimating phylogenetic β-diversity, particularly for communities in tropical or subtropical forests. In this study, we employed DNA barcodes combing with the nuclear region to construct the community phylogeny and examined the patterns of phylogenetic β-diversity of three mid-subtropical evergreen broad-leaved forests (EBLFs) in South China. Three phylogenetic construction methods were performed, including a Phylomatic-generated tree and two ML trees based on the combination of rbcL + matK + ITS with or without a constrained tree. Our results showed that the DNA barcodes including nuclear ITS constructed a highly resolved phylogenetic tree, but the application of a constrained tree had little influence on estimation of phylogenetic diversity metrics (mean pairwise distances and mean nearest taxon distances) based on branch lengths. Using both metrics and their standardized effect size metrics, we found that the patterns of phylogenetic β-diversity in mid-subtropical forests were non-random. There was a slight decline of phylogenetic β-diversity with increasing latitudes, but no trend was found along the altitude gradient. According to the analysis of variation partition, both environmental filtering and dispersion limitation could explain the variation of phylogenetic dissimilarity between communities in mid-subtropical EBLFs of China. Our results highlight the importance of neutrality and the niche conservatism in structuring the patterns of species diversity in subtropical woody communities.
Leaf and Crown Optical Properties of Five Early-, Mid- and Late-Successional Temperate Tree Species and Their Relation to Sapling Light Demand
Authors: Hagemeier Marc , Leuschner Christoph
Published: October 2019

The optical properties of leaves and canopies determine the availability of radiation for photosynthesis and the penetration of light through tree canopies. How leaf absorptance, reflectance and transmittance and radiation transmission through tree canopies change with forest succession is not well understood. We measured the leaf optical properties in the photosynthetically active radiation (PAR) range of five Central European early-, mid- and late-successional temperate broadleaf tree species and studied the minimum light demand of the lowermost shade leaves and of the species’ offspring. Leaf absorptance in the 350–720 nm range varied between c. 70% and 77% in the crown of all five species with only a minor variation from the sun to the shade crown and between species. However, specific absorptance (absorptance normalized by mass per leaf area) increased about threefold from sun to shade leaves with decreasing leaf mass area (LMA) in the late-successional species (Carpinus betulus L., Tilia cordata Mill., Fagus sylvatica L.), while it was generally lower in the early- to mid-successional species (Betula pendula Roth, Quercus petraea (Matt.)Liebl.), where it changed only a little from sun to shade crown. Due to a significant increase in leaf area index, canopy PAR transmittance to the forest floor decreased from early- to late-successional species from ~15% to 1%–3% of incident PAR, linked to a decrease in the minimum light demand of the lowermost shade leaves (from ~20 to 1%–2%) and of the species’ saplings (from ~20 to 3%–4%). The median light intensity on the forest floor under a closed canopy was in all species lower than the saplings’ minimum light demand. We conclude that the optical properties of the sun leaves are very similar among early-, mid- and late-successional tree species, while the shade leaves of these groups differ not only morphologically, but also in terms of the resource investment needed to achieve high PAR absorptance.
Evaluation of Ionic Liquids as In Situ Extraction Agents during the Alcoholic Fermentation of Carob Pod Extracts
Authors: Sanchez-Segado Sergio , Salar-García María José , Ortiz-Martínez Víctor Manuel , de los Ríos Antonia Pérez , Hernández-Fernández Francisco José , Lozano-Blanco Luis Javier
Published: October 2019

Anhydrous ethanol is a promising alternative to gasoline in fuel engines. However, since ethanol forms an azeotrope with water, high-energy-consumption separation techniques such as azeotropic distillation, extractive distillation, and molecular sieves are needed to produce anhydrous ethanol. This work discusses the potential development of an integrated process for bioethanol production using ionic liquids and Ceratonia siliqua as a carbohydrate source for further fermentation of the aqueous extracts. A four-stage counter-current system was designed to improve the sugar extraction yield to values close to 99%. The alcoholic fermentation of the extracts showed ethanol concentrations of 95 g/L using the microorganism Saccharomyces cerevisae. The production of anhydrous ethanol through extractive distillation with ethylene glycol was simulated using CHEMCAD software, with an energy consumption of 13.23 MJ/Kg of anhydrous ethanol. Finally, several ionic liquids were analyzed and are proposed as potential solvents for the recovery of bioethanol for the design of an integrated extraction–fermentation–separation process, according to their ability to extract ethanol from aqueous solutions and their biocompatibility with the microorganism used in this study.
Changes in Metabolic Profiles of Yellowtail (Seriola quinqueradiata) Muscle during Cold Storage as a Freshness Evaluation Tool Based on GC-MS Metabolomics
Authors: Mabuchi Ryota , Adachi Miwako , Ishimaru Ayaka , Zhao Huiqing , Kikutani Haruka , Tanimoto Shota
Published: October 2019

We applied metabolomics to the evaluation of yellowtail muscle as a new freshness evaluation method for fish meat. Metabolites from yellowtail ordinary and dark muscle (DM) stored at 0 °C and 5 °C were subjected to metabolomics for primary metabolites based on gas chromatography-mass spectrometry (GC-MS). For the annotated metabolites, we created statistically significant models for storage time prediction for all storage conditions by orthogonal partial least squares analysis, using storage time as the y-variable. DM is difficult to evaluate using the K value method, the predominant existing freshness evaluation method. However, in the proposed method, the metabolic component profiles of DM changed depending on storage time. Important metabolites determined from variables important for prediction (VIP) values included various metabolites, such as amino acids and sugars, in addition to nucleic-acid-related substances, especially inosine and hypoxanthine. Therefore, metabolomics, which comprehensively analyses different molecular species, has potential as a new freshness evaluation method that can objectively evaluate conditions of stored fish meat.
Insights into the Allergenic Potential of the Edible Yellow Mealworm (Tenebrio molitor)
Authors: Barre Annick , Pichereaux Carole , Velazquez Esmeralda , Maudouit Agathe , Simplicien Mathias , Garnier Lorna , Bienvenu Françoise , Bienvenu Jacques , Burlet-Schiltz Odile , Auriol Cédric , Benoist Hervé , Rougé Pierre
Published: October 2019

The edible yellow mealworm (Tenebrio molitor), contains an extremely diverse panel of soluble proteins, including proteins with structural functions such as muscle proteins, as well as proteins involved in metabolic functions such as enzymes. Most of these proteins display a more or less pronounced allergenic character toward previously sensitized people, especially people allergic to shrimps and other shellfish. A mass spectrometry approach following the separation of a mealworm protein, extracted by sodiumdodecyl sulfate-polyacrylamide gel electrophoresis, allowed us to identify up to 106 distinct protein fractions including molecules with structural and functional functions, susceptible to developing an allergenic potential due to the possibility of immunoglobulin E-binding cross-reactions with their counterparts occurring in shellfish. In this respect, most of the sera from people allergic to shrimps reacted with the mealworm protein extract in Western blot experiments. Moreover, the potential mealworm allergens triggered the in vitro degranulation of rat leukemic basophils transfected with the human high-affinity IgE receptor (FcεRI), upon sensitization by the IgE-containing sera from people allergic to shrimps and other shellfish foods. Owing to the large repertoire of IgE-binding cross-reacting allergens the yellow mealworm shares with other phylogenetically-related groups of arthropods, it would seem prudent to inform the consumers, especially those allergic to shellfish, by appropriate labeling on edible mealworm packages about the potential risk of developing an allergic reaction.
Frameshift Variant in MFSD12 Explains the Mushroom Coat Color Dilution in Shetland Ponies
Authors: Tanaka Jocelyn , Leeb Tosso , Rushton James , Famula Thomas R. , Mack Maura , Jagannathan Vidhya , Flury Christine , Bachmann Iris , Eberth John , McDonnell Sue M. , Penedo Maria Cecilia T. , Bellone Rebecca R.
Published: October 2019

Mushroom is a unique coat color phenotype in Shetland Ponies characterized by the dilution of the chestnut coat color to a sepia tone and is hypothesized to be a recessive trait. A genome wide association study (GWAS), utilizing the Affymetrix 670K array (MNEc670k) and a single locus mixed linear model analysis (EMMAX), identified a locus on ECA7 for further investigation (Pcorrected = 2.08 × 10−10). This locus contained a 3 Mb run of homozygosity in the 12 mushroom ponies tested. Analysis of high throughput Illumina sequencing data from one mushroom Shetland pony compared to 87 genomes from horses of various breeds, uncovered a frameshift variant, p.Asp201fs, in the MFSD12 gene encoding the major facilitator superfamily domain containing 12 protein. This variant was perfectly concordant with phenotype in 96 Shetland Ponies (P = 1.15 × 10−22), was identified in the closely related Miniature Horse for which the mushroom phenotype is suspected to occur (fmu = 0.02), and was absent in 252 individuals from seven additional breeds not reported to have the mushroom phenotype. MFSD12 is highly expressed in melanocytes and variants in this gene in humans, mice, and dogs impact pigmentation. Given the role of MFSD12 in melanogenesis, we propose that p.Asp201fs is causal for the dilution observed in mushroom ponies.
Measuring the Fate of Compost-Derived Phosphorus in Native Soil below Urban Gardens
Authors: Small Gaston E. , Osborne Sara , Shrestha Paliza , Kay Adam
Published: October 2019

The heavy reliance on compost inputs in urban gardening provides opportunities to recycle nutrients from the urban waste stream, but also creates potential for buildup and loss of soil phosphorus (P). We previously documented P in leachate from raised-bed garden plots in which compost had been applied, but the fate of this P is not known. Here, we measured P concentrations in soils below four or six-year-old urban garden plots that were established for research. We hypothesize that the soil P concentration and depth of P penetration will increase over time after gardens are established. Soil cores were collected in five garden plots of each age and quantified for inorganic weakly exchangeable P. Inorganic weakly exchangeable P was significantly elevated in native soil below garden plots (>35 cm deep) relative to reference soil profiles, and excess P decreased with increasing depth, although differences between garden plots of different ages were not significant. Our analysis shows that excess P from compost accumulates in native soil below urban garden plots. While urban agriculture has the potential to recycle P in urban ecosystems, over-application of compost has the potential to contribute to soil and water pollution.
Toileting Behaviors Related to Urination in Women: A Scoping Review
Authors: Wu Chen , Xue Kaikai , Palmer Mary H.
Published: October 2019

This scoping review explores the state of science regarding women’s toileting behaviors, gaps in knowledge, and areas for future research. Online databases were searched to identify papers published in English between January 2010 through July 2019; the search identified 25 articles. The Toileting Behaviors–Women’s Elimination Behaviors scale has been published in four validated language versions and used in 17 of the 25 studies. The most frequent behaviors include concern about public toilet cleanliness, delaying urination when busy or away from home, and using different toileting postures at and away from home. Determinants of toileting behaviors include environmental factors, chronic health conditions, and cognitive/psychological factors. Associations were found between toileting behaviors and lower urinary tract symptoms and between toileting postures and uroflowmetric parameters and post-void residual volume. Strategies that address modifiable determinants of toileting behaviors should be developed and tested in future research. Furthermore, little is known about the toileting behaviors and bladder health in older women and women from developing countries. Rigorous studies are needed to better understand the underlying mechanisms of toileting behaviors, the nature of associations between toileting behaviors and lower urinary tract symptoms, and effects of the environment on women’s toileting behaviors.
Identifying Driving Factors of Jiangsu’s Regional Sulfur Dioxide Emissions: A Generalized Divisia Index Method
Authors: Yang Junliang , Shan Haiyan
Published: October 2019

The Chinese government has made some good achievements in reducing sulfur dioxide emissions through end-of-pipe treatment. However, in order to implement the stricter target of sulfur dioxide emission reduction during the 13th “Five-Year Plan” period, it is necessary to find a new solution as quickly as possible. Thus, it is of great practical significance to identify driving factors of regional sulfur dioxide emissions to formulate more reasonable emission reduction policies. In this paper, a distinctive decomposition approach, the generalized Divisia index method (GDIM), is employed to investigate the driving forces of regional industrial sulfur dioxide emissions in Jiangsu province and its three regions during 2004–2016. The contribution rates of each factor to emission changes are also assessed. The decomposition results demonstrate that: (i) the factors promoting the increase of industrial sulfur dioxide emissions are the economic scale effect, industrialization effect, and energy consumption effect, while technology effect, energy mix effect, sulfur efficiency effect, energy intensity effect, and industrial structure effect play a mitigating role in the emissions; (ii) energy consumption effect, energy mix effect, technology effect, sulfur efficiency effect, and industrial structure effect show special contributions in some cases; (iii) industrial structure effect and energy intensity effect need to be further optimized.
Factors Influencing Unintended Pregnancies amongst Adolescent Girls and Young Women in Cambodia
Authors: Rizvi Farwa , Williams Joanne , Hoban Elizabeth
Published: October 2019

Background: Unintended pregnancies in Cambodian youth are a major reproductive health concern with detrimental personal and socioeconomic consequences. A social ecological model was used to identify sociodemographic factors potentially associated with unintended pregnancies, and an analysis of data from the 2014 Cambodian Demographic and Health Survey was used to determine associations. Methods: Weighted data were analysed using multiple logistic regression analyses for 3406 Cambodian sexually active single, in union or married females aged 15–29 years. Results: The prevalence of unintended pregnancy was 12.3%. Unintended pregnancy was significantly associated with younger age groups (15–24 years), multiparity, history of abortion, and current use of modern contraceptive methods. All women had an increased likelihood of unintended pregnancy when the husband alone or someone else in the household made decisions about their access to healthcare. Conclusion: The burden of unintended pregnancies is associated with young age, multiparity, history of abortions, unemployment, and low autonomy for accessing healthcare. Multi-pronged, holistic reproductive and sexual health program interventions are needed to increase literacy and accessibility to modern contraception and to raise awareness about women’s health and status in Cambodia.
Environmental Impact of Burning Composite Materials Used in Aircraft Construction on the Air
Authors: Vajdová Iveta , Jenčová Edina , Szabo Stanislav , Melníková Lucia , Galanda Jozef , Dobrowolska Malgorzata , Ploch Jindřich
Published: October 2019

The negative impact of air incidents and emergency situations results from the leakage of liquids into the soil and water and the leakage of flue gases and combustion products of aircraft structural materials into the air during fires. This article deals with air pollution caused by the combustion of composite materials commonly used in general aviation. Samples of composite materials of aircraft registered in the Czech Republic were selected. These samples of composite materials were tested for flammability, according to ISO 5660-1:2002 Reaction to fire tests—Heat Release, smoke production and mass loss rate (ISO—International Organization for Standardization). Total smoke release and total oxygen consumed were assessed in this study, both of which have a significant impact on air quality in the case of an air incident. Based on the results of the research, differences resulting from the diversity of the structures of the tested composite materials were found. The most hazardous composite material was evaluated from the point of view of its impact on air quality during combustion.
Distributional Regression Techniques in Socioeconomic Research on the Inequality of Health with an Application on the Relationship between Mental Health and Income
Authors: Silbersdorff Alexander , Schneider Kai Sebastian
Published: October 2019

This study addresses the much-discussed issue of the relationship between health and income. In particular, it focuses on the relation between mental health and household income by using generalized additive models of location, scale and shape and thus employing a distributional perspective. Furthermore, this study aims to give guidelines to applied researchers interested in taking a distributional perspective on health inequalities. In our analysis we use cross-sectional data of the German socioeconomic Panel (SOEP). We find that when not only looking at the expected mental health score of an individual but also at other distributional aspects, like the risk of moderate and severe mental illness, that the relationship between income and mental health is much more pronounced. We thus show that taking a distributional perspective, can add to and indeed enrich the mostly mean-based assessment of existent health inequalities.
Parental Approach to the Management of Childhood Fever: Differences between Health Professional and Non-Health Professional Parents
Authors: Villarejo-Rodríguez María Gloria , Rodríguez-Martín Beatriz
Published: October 2019

Fever is responsible for 30% of pediatric consultations at primary care services. The aim of this study was to explore the parental approach to fever in children aged between 0 and 12 years old by both health professional and non-health professional parents. A qualitative study based on grounded theory was performed. Focus groups were conducted, segmented by sex, place of residence, and healthcare training, using a triangulated sample (theoretical and snowball sampling) of parents of children aged between 0 and 12 years who were treated for fever at primary care emergency services. The constant comparative method and a process of coding was used for the analysis. The study findings reveal that the health training of parents, their former experience, family pressures, the age of the child, and the parents’ work outside the home, all influenced how they approached fever management. These findings could be incorporated into clinical practice to improve care and compliance with fever treatment.
The Contextual Effect of Area-Level Unemployment Rate on Lower Back Pain: A Multilevel Analysis of Three Consecutive Surveys of 962,586 Workers in Japan
Authors: Ikeda Takaaki , Sugiyama Kemmyo , Aida Jun , Tsuboya Toru , Osaka Ken
Published: October 2019

This study examined the associations between area-level unemployment rates and lower back pain using large-scale data provided by the Japanese working population. We analyzed data from a nationally representative, repeated, cross-sectional study across three waves from 2010, 2013, and 2016 in 47 Japanese subnational level areas. Workers aged 18–64 years (n = 962,586) were eligible to participate in the study. A multilevel logistic model was used to examine the association between the unemployment rate and lower back pain. The self-report of lower back pain was a dependent variable. The prefecture-level unemployment rate was analyzed as an independent variable, adjusted for individual-level covariates (e.g., gender, age, socioeconomic status). After adjusting for all covariates, the main effect of the prefecture-level unemployment rate was statistically significant: the odds ratio (OR) (95% credible interval (CrI)) was 1.01 (1.002, 1.03). Additionally, the OR (95% CrI) for the interaction between gender and the prefecture-level unemployment rate was 1.02 (1.01, 1.03) indicating that women were more affected by area-level employment status than men. In conclusion, a significant association between the unemployment rate and lower back pain was observed in the Japanese working population. Women were more sensitive to the unemployment rate.
Effects of Slope Ecological Restoration on Runoff and Its Response to Climate Change
Authors: He Shan , Qin Tianling , Liu Fang , Liu Shanshan , Dong Biqiong , Wang Jianwei , Nie Hanjiang
Published: October 2019

Slope ecological restoration and climate change are important factors affecting the hydrological processes of the Huangshui River Basin in Qinghai province, China. How to quantitatively identify the impact of slope ecological restoration on runoff and whether slope ecological restoration can mitigate the impact of future climate change on runoff are both very important. In this paper, the Huangshui River above the center of Minhe county was taken as the research area, and the Pinus tabulaeformis and shrubs were taken as the main forest land types of slope ecological restoration. First, based on the law of forest land variation, the construction scales of slope ecological restoration in different periods were identified. The influence of slope ecological restoration on runoff was then quantitatively evaluated by using a distributed hydrological model. Second, the future climate scenarios of five general circulation models (GCMs) under three representative concentration pathways (RCPs) (i.e., RCP2.6, RCP4.5, and RCP8.5) from 2021 to 2050 were selected and modified by model integration. Combined with the slope ecological restoration scenarios, the influence of slope ecological restoration on runoff under future climate scenarios was explored. The results showed that the effect of slope ecological restoration was significant. Compared with 1980, the area of slope ecological restoration increased by 24% in 2017. Under the present climate conditions (1960–2017), different periods of slope ecological restoration have an effect on the process of runoff in the wet season (June, July, August, and September) and dry season (January, February, March, and December), which eliminates the maximum, replenishes the minimum, and reduces the variability of runoff processes in the watershed. Under the future climate scenario (2021–50), slope ecological restoration will reduce runoff. On the other hand, climate change will increase runoff, and the combination of the two effects will have a certain offsetting effect. On the whole, comparing the influence of slope ecological restoration on the runoff process with that of climate change in different seasons, due to the main influence of slope ecological restoration, the runoff decreased by about 55% in the temperate season (April, May, October, and November), and increased by about 50% in the dry season or wet season due to the main influence of future climate scenarios.
Two Approaches to Increase Physical Activity for Preschool Children in Child Care Centers: A Matched-Pair Cluster-Randomized Trial
Authors: Tandon Pooja S. , Downing Katherine L. , Saelens Brian E. , Christakis Dimitri A.
Published: October 2019

Early childhood education settings are critical for promoting physical activity (PA) but intervention effects are often small. The aim of this study was to develop, test, and compare two approaches to increasing physical activity among preschoolers at child care centers: one focused on a teacher-led PA curriculum (Active Play!) and the other on increasing outdoor child-initiated free play time (Outdoor Play!). We conducted a matched-pair cluster-randomized study in 10 centers in and around Seattle, WA, USA (n = 97 children, mean age 4.6). Pre- and post-intervention data were collected from observations and accelerometers. At pre-intervention, 19% of Active Play! and 25% of Outdoor Play! children achieved >120 min/day of PA during child care. The total opportunity for PA increased in both interventions (Active Play! = 11 min/day; Outdoor Play! = 14 min/day), with the largest increase in outdoor child-initiated free playtime (Active Play! = 19 min/day; Outdoor Play! = 24 min/day). No changes in sedentary time, light or moderate- to vigorous-intensity PA (MVPA) were observed in either intervention and there was no difference between interventions in the percentage of children attaining more than 120 min/day of PA. A small (<3 min/day) relative increase in teacher-led outdoor activity was observed in the Active Play! intervention. Both intervention strategies led to an increase in active play opportunities, predominantly outdoors, but neither was able to substantially increase the intensity and/or duration of children’s PA. Future studies are needed to better understand and inform sustainable approaches to increase PA in early learning settings.
‘There You Enjoy Life, Here You Work’: Brazilian and Dominican Immigrants’ Views on Work and Health in the U.S.
Authors: Brinkerhoff Cristina Araujo , Siqueira C. Eduardo , Negrón Rosalyn , Tracy Natalicia , Troncoso Lama Magalis , Sprague Martinez Linda
Published: October 2019

Structural inequalities in the U.S. work environment place most immigrants in low paying, high-risk jobs. Understanding how work experiences and influence the health of different immigrant populations is essential to address disparities. This article explores how Brazilian and Dominican immigrants feel about their experiences working in the U.S. and how the relationship between work and culture might impact their health. In partnership with the Dominican Development Center and the Brazilian Worker Center, we held five cultural conversations (CCs) with Brazilians (n = 48) and five with Dominicans (n = 40). CCs are participatory, unstructured groups facilitated by representatives from or embedded in the community. Brazilian immigrants focused on physical health and the American Dream while Dominicans immigrants emphasized concerns about the influence of work on mental health. Dominicans’ longer tenure in the U.S. and differences in how Brazilians and Dominicans are racialized in the region might account for the variation in perspectives between groups. Future studies should further investigate the relationship between health and how immigrants’ work lives are shaped by culture, race and immigrant status.
A Scoping Review and Conceptual Model of Social Participation and Mental Health among Refugees and Asylum Seekers
Authors: Niemi Maria , Manhica Hélio , Gunnarsson David , Ståhle Göran , Larsson Sofia , Saboonchi Fredrik
Published: October 2019

Social participation plays a key role in the integration of refugees and asylum seekers into their host societies, and is also closely tied to the mental health of those populations. The aim of this scoping review was to study how the concept of social participation is described in empirical research, and how it is associated with mental health outcomes. Methods: In total, 64 studies were identified through searches in PubMed, PsycInfo, and Sociological Abstracts. These studies describe various forms of social participation among refugees and asylum seekers, and 33 of them also addressed various forms of mental health outcomes. Results: The identified studies described forms and conditions of social participation—both in the host country and transnationally—that could be synthesized into three broad dimensions: (1) Regulatory frameworks, conditions and initiatives; (2) Established societal organizations and social structures; and (3) Community organized groups. Each of these consisted of several sub-domains. The identified dimensions of social participation were also associated with psychosocial well-being and decreased psychological distress. Conclusions: There is a need for policies to enable and support the participation of refugees and asylum seekers in various dimensions of social structures in host societies. Social participation enhances resilience, re-establishes social lives, and acts as a protective factor against poor mental health outcomes.
Influence of Two-Stage Combinations of Constructed Wetlands on the Removal of Antibiotics, Antibiotic Resistance Genes and Nutrients from Goose Wastewater
Authors: Huang Xiaofeng , Luo Yi , Liu Zuolan , Zhang Changlian , Zhong Hang , Xue Jiajia , Wang Qigui , Zhu Zhiping , Wang Chao
Published: October 2019

Antibiotic and antibiotic resistance genes (ARGs) have been considered as emerging environmental contaminants and possess potential crisis to global public health. However, little is known about the differences between various configurations of two-stage combinations of constructed wetlands (CWs) on antibiotics and ARG removal from wastewater. In the study, three configurations of two-stage hybrid CWs (horizontal subsurface flow-down-flow vertical subsurface flow CWs, HF-DVF; horizontal subsurface flow-up-flow vertical subsurface flow CWs, HF-UVF; down-flow vertical subsurface flow-up-flow vertical subsurface flow CWs, DVF-UVF) were operated to evaluate their ability to remove high-concentration antibiotics (tilmicosin—TMS and doxycycline—DOC), ARGs (seven tet genes and three erm genes), intI1, 16S rRNA, and nutrients from goose wastewater. The results showed that all three hybrid CWs could remove more than 98% of TMS and DOC from wastewater, without significant difference among treatments (p > 0.05). For ARGs, DVF-UVF showed significantly higher removal efficiencies of intI1, ermB, ermC, ermF, tetW, and tetG compared to HF-UVF (p < 0.05), mainly because they might remove and arrest growth of bacteria. The relatively high removal efficiencies of NH4+-N, NO3—N, and NO2--N were also observed from DVF-UVF, ranging from 87% to 95% (p > 0.05), indicating that anaerobic ammonium oxidation (anammox) might be established in the CWs. Our results demonstrate that the removal performances of antibiotics using two-stage hybrid CWs are not affected by the combined configuration, whereas the combination of DVF and UVF CWs perform better on the removal of ARGs and nutrients compared with HF-DVF and HF-UVF CWs.
Work-Related Stress, Physio-Pathological Mechanisms, and the Influence of Environmental Genetic Factors
Authors: Cannizzaro Emanuele , Ramaci Tiziana , Cirrincione Luigi , Plescia Fulvio
Published: October 2019

Work-related stress is a growing health problem in modern society. The stress response is characterized by numerous neurochemicals, neuroendocrine and immune modifications that involve various neurological systems and circuits, and regulation of the gene expression of the different receptors. In this regard, a lot of research has focused the attention on the role played by the environment in influencing gene expression, which in turn can control the stress response. In particular, genetic factors can moderate the sensitivities of specific types of neural cells or circuits mediating the imprinting of the environment on different biological systems. In this current review, we wish to analyze systematic reviews and recent experimental research on the physio-pathological mechanisms that underline stress-related responses. In particular, we analyze the relationship between genetic and epigenetic factors in the stress response.
Analysis of the Association between Running Performance and Game Performance Indicators in Professional Soccer Players
Authors: Modric Toni , Versic Sime , Sekulic Damir , Liposek Silvester
Published: October 2019

Running performance (RP) and game performance indicators (GPI) are important determinants of success in soccer (football), but there is an evident lack of knowledge about the possible associations between RP and GPI. This study aimed to identify associations between RP and GPI in professional soccer players and to compare RP and GPI among soccer playing positions. One hundred one match performances were observed over the course of half of a season at the highest level of national competition in Croatia. Players (mean ± SD, age: 23.85 ± 2.88 years; body height: 183.05 ± 8.88 cm; body mass: 78.69 ± 7.17 kg) were classified into five playing positions (central defenders (n = 26), full-backs (n = 24), central midfielders (n = 33), wide midfielders (n = 10), and forwards (n = 8). RP, as measured by global positioning system, included the total distance covered, distance covered in five speed categories (walking, jogging, running, high-speed running, and maximal sprinting), total number of accelerations, number of high-intensity accelerations, total number of decelerations, and number of high-intensity decelerations. The GPI were collected by the position-specific performance statistics index (InStat index). The average total distance was 10,298.4 ± 928.7 m, with central defenders having the shortest and central midfielders having the greatest covered distances. The running (r = 0.419, p = 0.03) and high-intensity accelerations (r = 0.493, p = 0.01) were correlated with the InStat index for central defenders. The number of decelerations of full-backs (r = −0.43, p = 0.04) and the distance covered during sprinting of forwards (r = 0.80, p = 0.02) were associated with their GPI obtained by InStat index. The specific correlations between RP and GPI should be considered during the conditioning process in soccer. The soccer training should follow the specific requirements of the playing positions established herein, which will allow players to meet the game demands and to perform successfully.
Levels of Physical Activity, Obesity and Related Factors in Young Adults Aged 18–30 During 2009–2017
Authors: Laredo-Aguilera José Alberto , Cobo-Cuenca Ana Isabel , Santacruz-Salas Esmeralda , Martins María Manuela , Rodríguez-Borrego María Aurora , López-Soto Pablo Jesús , Carmona-Torres Juan Manuel
Published: October 2019

The objective of this study was to analyze the temporal trend of physical activity and body mass index in young adults aged 18–30 in Spain and to ascertain their relationship with sociodemographic and psychosocial variables in the period of 2009–2017. Methods: A descriptive study with a sample of 10,061 young adults aged 18–30 years was performed. The data were obtained from the European Health Survey in Spain in 2009 and 2014 and the National Health Survey in 2011/2012 and 2017. The chi-square test was used for qualitative variables, and multiple linear regression analysis was performed for physical activity. Results: Sedentary levels had decreased in 2017 as compared to 2011/2012 (p < 0.001); smokers were more sedentary than non-smokers (p < 0.001); men were more active than women (p < 0.001); and the year with the highest physical activity was 2014. Body mass index in the total sample increased from 2009 to 2017 (p < 0.01), showing a significant increase in obesity in women (p < 0.05) and no difference in men (p ≥ 0.05). Conclusions: In the period 2011/2012–2017, the sedentary lifestyle of young adults was reduced and physical activity was increased, with men being more active than women.
Depicting Conformational Ensembles of α-Synuclein by Single Molecule Force Spectroscopy and Native Mass Spectroscopy
Authors: Corti Roberta , Marrano Claudia A. , Salerno Domenico , Brocca Stefania , Natalello Antonino , Santambrogio Carlo , Legname Giuseppe , Mantegazza Francesco , Grandori Rita , Cassina Valeria
Published: October 2019

Description of heterogeneous molecular ensembles, such as intrinsically disordered proteins, represents a challenge in structural biology and an urgent question posed by biochemistry to interpret many physiologically important, regulatory mechanisms. Single-molecule techniques can provide a unique contribution to this field. This work applies single molecule force spectroscopy to probe conformational properties of α-synuclein in solution and its conformational changes induced by ligand binding. The goal is to compare data from such an approach with those obtained by native mass spectrometry. These two orthogonal, biophysical methods are found to deliver a complex picture, in which monomeric α-synuclein in solution spontaneously populates compact and partially compacted states, which are differently stabilized by binding to aggregation inhibitors, such as dopamine and epigallocatechin-3-gallate. Analyses by circular dichroism and Fourier-transform infrared spectroscopy show that these transitions do not involve formation of secondary structure. This comparative analysis provides support to structural interpretation of charge-state distributions obtained by native mass spectrometry and helps, in turn, defining the conformational components detected by single molecule force spectroscopy.
Determination of the Pathological Features of NPC1 Variants in a Cellular Complementation Test
Authors: Feng Xiao , Cozma Claudia , Pantoom Supansa , Hund Christina , Iwanov Katharina , Petters Janine , Völkner Christin , Bauer Claudia , Vogel Florian , Bauer Peter , Weiss Frank U. , Lerch Markus M. , Knospe Anne-Marie , Hermann Andreas , Frech Moritz J. , Luo Jiankai , Rolfs Arndt , Lukas Jan
Published: October 2019

Niemann-Pick Type C (NP-C) is a rare disorder of lipid metabolism caused by mutations within the NPC1 and NPC2 genes. NP-C is a neurovisceral disease leading to a heterogeneous, multisystemic spectrum of symptoms in those affected. Until now, there is no investigative tool to demonstrate the significance of single variants within the NPC genes. Hence, the aim of the study was to establish a test that allows for an objective assessment of the pathological potential of NPC1 gene variants. Chinese hamster ovary cells defective in the NPC1 gene accumulate cholesterol in lysosomal storage organelles. The cells were transfected with NPC1-GFP plasmid vectors carrying distinct sequence variants. Filipin staining was used to test for complementation of the phenotype. The known variant p.Ile1061Thr showed a significantly impaired cholesterol clearance after 12 and 24 h compared to the wild type. Among the investigated variants, p.Ser954Leu and p.Glu1273Lys showed decelerated cholesterol clearance as well. The remaining variants p.Gln60His, p.Val494Met, and p.Ile787Val showed a cholesterol clearance indistinguishable from wild type. Further, p.Ile1061Thr acquired an enhanced clearance ability upon 25-hydroxycholesterol treatment. We conclude that the variants that caused an abnormal clearance phenotype are highly likely to be of clinical relevance. Moreover, we present a system that can be utilized to screen for new drugs.
Functional Prediction of Candidate MicroRNAs for CRC Management Using in Silico Approach
Authors: Fadaka Adewale Oluwaseun , Pretorius Ashley , Klein Ashwil
Published: October 2019

Approximately 30–50% of malignant growths can be prevented by avoiding risk factors and implementing evidence-based strategies. Colorectal cancer (CRC) accounted for the second most common cancer and the third most common cause of cancer death worldwide. This cancer subtype can be reduced by early detection and patients’ management. In this study, the functional roles of the identified microRNAs were determined using an in silico pipeline. Five microRNAs identified using an in silico approach alongside their seven target genes from our previous study were used as datasets in this study. Furthermore, the secondary structure and the thermodynamic energies of the microRNAs were revealed by Mfold algorithm. The triplex binding ability of the oligonucleotide with the target promoters were analyzed by Trident. Finally, evolutionary stage-specific somatic events and co-expression analysis of the target genes in CRC were analyzed by SEECancer and GeneMANIA plugin in Cytoscape. Four of the five microRNAs have the potential to form more than one secondary structure. The ranges of the observed/expected ratio of CpG dinucleotides of these genes range from 0.60 to 1.22. Three of the candidate microRNA were capable of forming multiple triplexes along with three of the target mRNAs. Four of the total targets were involved in either early or metastatic stage-specific events while three other genes were either a product of antecedent or subsequent events of the four genes implicated in CRC. The secondary structure of the candidate microRNAs can be used to explain the different degrees of genetic regulation in CRC due to their conformational role to modulate target interaction. Furthermore, due to the regulation of important genes in the CRC pathway and the enrichment of the microRNA with triplex binding sites, they may be a useful diagnostic biomarker for the disease subtype.
In Silico and In Vitro Assessment of Portuguese Oyster (Crassostrea angulata) Proteins as Precursor of Bioactive Peptides
Authors: Gomez Honey Lyn R. , Peralta Jose P. , Tejano Lhumen A. , Chang Yu-Wei
Published: October 2019

In this study, the potential bioactivities of Portuguese oyster (Crassostrea angulata) proteins were predicted through in silico analyses and confirmed by in vitro tests. C. angulata proteins were characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and identified by proteomics techniques. Hydrolysis simulation by BIOPEP-UWM database revealed that pepsin (pH > 2) can theoretically release greatest amount of bioactive peptides from C. angulata proteins, predominantly angiotensin I-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibitory peptides, followed by stem bromelain and papain. Hydrolysates produced by pepsin, bromelain and papain have shown ACE and DPP-IV inhibitory activities in vitro, with pepsin hydrolysate (PEH) having the strongest activity of 78.18% and 44.34% at 2 mg/mL, respectively. Bioactivity assays of PEH fractions showed that low molecular weight (MW) fractions possessed stronger inhibitory activity than crude hydrolysate. Overall, in vitro analysis results corresponded with in silico predictions. Current findings suggest that in silico analysis is a rapid method to predict bioactive peptides in food proteins and determine suitable enzymes for hydrolysis. Moreover, C. angulata proteins can be a potential source of peptides with pharmaceutical and nutraceutical application.
N-Glycosylation of TREK-1/hK2P2.1 Two-Pore-Domain Potassium (K2P) Channels
Authors: Wiedmann Felix , Schlund Daniel , Faustino Francisco , Kraft Manuel , Ratte Antonius , Thomas Dierk , Katus Hugo A. , Schmidt Constanze
Published: October 2019

Mechanosensitive hTREK-1 two-pore-domain potassium (hK2P2.1) channels give rise to background currents that control cellular excitability. Recently, TREK-1 currents have been linked to the regulation of cardiac rhythm as well as to hypertrophy and fibrosis. Even though the pharmacological and biophysical characteristics of hTREK-1 channels have been widely studied, relatively little is known about their posttranslational modifications. This study aimed to evaluate whether hTREK-1 channels are N-glycosylated and whether glycosylation may affect channel functionality. Following pharmacological inhibition of N-glycosylation, enzymatic digestion or mutagenesis, immunoblots of Xenopus laevis oocytes and HEK-293T cell lysates were used to assess electrophoretic mobility. Two-electrode voltage clamp measurements were employed to study channel function. TREK-1 channel subunits undergo N-glycosylation at asparagine residues 110 and 134. The presence of sugar moieties at these two sites increases channel function. Detection of glycosylation-deficient mutant channels in surface fractions and recordings of macroscopic potassium currents mediated by these subunits demonstrated that nonglycosylated hTREK-1 channel subunits are able to reach the cell surface in general but with seemingly reduced efficiency compared to glycosylated subunits. These findings extend our understanding of the regulation of hTREK-1 currents by posttranslational modifications and provide novel insights into how altered ion channel glycosylation may promote arrhythmogenesis.
Effect of Single Amino Acid Substitutions by Asn and Gln on Aggregation Properties of Bence-Jones Protein BIF
Authors: Timchenko Maria , Abdullatypov Azat , Kihara Hiroshi , Timchenko Alexander
Published: October 2019

The nature of renal amyloidosis involving Bence-Jones proteins in multiple myeloma is still unclear. The development of amyloidosis in neurodegenerative diseases is often associated with a high content of asparagine and glutamine residues in proteins forming amyloid deposits. To estimate the influence of Asn and Gln residues on the aggregation of Bence-Jones protein BIF, we obtained recombinant BIF and its mutants with the substitution of Tyr187→Asn (Y187N) in α-helix of CL domain, Lys170→Asn (K170N) and Ser157→Gln (S157Q) in CL domain loops, Arg109→Asn in VL-CL linker (R109N) and Asp29→Gln in VL domain loop (D29Q). The morphology of protein aggregates was studied at pH corresponding to the conditions in bloodstream (pH 7.2), distal (pH 6.5) and proximal renal tubules (pH 4.5) by atomic force microscopy (AFM) and small-angle X-ray scattering (SAXS). The Lys170→Asn replacement almost completely inhibits amyloidogenic activity. The Y187N forms fibril-like aggregates at all pH values. The Arg109→Asn replacement resulted in formation of fibril-like structures at pH 7.2 and 6.5 while the substitutions by Gln provoked formation of those structures only at pH 7.2. Therefore, the amyloidogenic properties are highly dependent on the location of Asn or Gln.