bluuebunny/crossref_metadata_2025 huggingface dataset:
Anisodamine (654-1/654-2) ameliorates septic kidney injury in rats by inhibiting inflammation and apoptosis
Authors: Liu Dong , Tang Fei , Zhang Li , Wan Feng , Xu Li-Yue , Zhang Jing-Nan , Zhao Xiao-Lan , Ao Hui , Peng Cheng
Published: September 2024

Introduction

To investigate the protective effects of anisodamine (654-1/654-2) against acute kidney injury (AKI) in LPS-induced septic shock rats and explore its molecular mechanisms.

Methods

56 rats were randomly divided into 8 groups: control, LPS, LPS + 654-1, and LPS + 654-2 (1.25, 2.5 and 5 mg/kg). The model was evaluated by monitoring MAP, HR, and plasma LD levels. ELISA and biochemical assay kits were used to measure the levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and kidney injury markers (BUN and CRE). Additionally, RNA-seq and bioinformatic analysis were performed to explore the mechanism of action of 654-1/654-2, and verification was conducted by western blotting and RT-PCR.

Results

654-1/654-2 significantly restored the levels of MAP, HR, and plasma LD in septic shock rats. Furthermore, 654-1/654-2 (5 mg/kg) effectively ameliorated LPS-induced kidney structural damage and exhibited a dose-dependent reduction in levels of inflammatory cytokines and kidney injury markers. In addition, RNA-seq, WB, and RT-PCR analyses revealed that 654-1/654-2 exerted its effects by inhibiting the expressions of the NF-κB and MAPK pathways and activating the Pi3K/Akt/Bcl-2 signaling pathway, thereby mitigating AKI.

Discussion

This study suggested that 654-1/654-2 could alleviate AKI in septic shock rats by improving inflammation invasion and cell apoptosis. Notably, 654-1/654-2 collectively suppressed inflammation response through the p38/JNK/AP-1/NF-κB pathway. Additionally, 654-1 promotes survival signaling via the Pi3K/Akt/Bcl-2 pathway, whereas 654-2 reduces apoptosis through the P53/Bax pathway. These findings provided a theoretical basis for the clinical application of 654-1/654-2 in treating organ damage caused by septic shock.
Traditional clinical symptoms and signs: Kampo pattern diagnosis in modern gastrointestinal disease
Authors: Zedler Paul , Büntzel Judith , Kuchta Kenny , Yamaoka Denichiro , Sato Nanoha , Watanabe Kenji , Cameron Silke
Published: September 2024

Introduction

In traditional Japanese Kampo medicine, a profound anamnesis is completed by clinical examination. The resulting clinical image forms the basis of the patient’s diagnosis pattern, including the recent mental, physical, and social contexts. Kampo questionnaires support pattern diagnosis and bridge traditional and Western medicine diagnoses.

Aims of the study

Traditional Kampo therapy is tailored to a specific body constitution, while Western medicine treatment is tailored to a specific disease. The aims of this study were to analyze whether traditional Kampo diagnosis is applicable to German patients and whether specific symptom patterns are characteristic for defined diseases.

Material and methods

This study validates for the first time a Kampo questionnaire adapted for German patients. The analysis focuses on patients with gastrointestinal diseases, the main field for Kampo medicines.

Results

In total, we prospectively included 251 participants; of those, 58 were cancer patients (23.1%), 35 had Crohn’s disease (13.9%), 18 had ulcerative colitis (7.2%), 17 had irritable bowel syndrome (6.8%), and 103 had other abdominal diseases (41%), as well as 20 German controls (8%). The patient population consisted of 144 female (57.4%) and 107 male (42.6%) participants. The median age was 65 years. The disease duration (average: 211 months) varied from 1 month (cancer patient) to 540 months (patient with Crohn’s disease). The scores for questions on the state of mind were significantly higher in patients with inflammatory bowel disease (IBD) as well as irritable bowel syndrome (IBS)—in comparison to the tumor and control groups. This was reflected in questions about abdominal discomfort, appetite, fecal habits, and cold sensation. Accordingly, symptoms of Qi (i.e., vital energy) deficiency were mostly observed in patients with chronic diseases such as Crohn’s disease and ulcerative colitis. Defined symptom combinations did not reflect conventional Western diagnosis.

Conclusion

Our study results show that symptom patterns are independent of the underlying disease. They rather depict the individual patient within an individual time frame. Traditional Kampo questionnaires were found to be valid for the analysis of a patients’ body constitution (sho) and serve as a guide for Kampo treatment. We propose that individual pattern diagnosis should be taken into account to help treatment individualization.
Formononetin triggers ferroptosis in triple-negative breast cancer cells by regulating the mTORC1/SREBP1/SCD1 pathway
Authors: Xie Dong , Jiang Yulang , Wang Huan , Zhu Lingyi , Huang Shuangqin , Liu Sheng , Zhang Weihong , Li Tian
Published: September 2024

Introduction

Triple-negative breast cancer (TNBC) is the most malignant type of breast cancer, and its prognosis is still the worst. It is necessary to constantly explore the pathogenesis and effective therapeutic targets of TNBC. Formononetin is an active ingredient with anti-tumor effects that we screened earlier. The main purpose of this study is to elucidate mechanism of the inhibitory effect of Formononetin on TNBC.

Methods

We conducted experiments through both in vivo and in vitro methodologies. The in vivo experiments utilized a nude mice xenotransplantation model, while the in vitro investigations employed two breast cancer cell lines, MDA-MB-231 and MDA-MB-468. Concurrently, ferroptosis associated proteins, lipid peroxide levels, and proteins related to the rapamycin complex 1 were analyzed in both experimental settings.

Results

In our study, Formononetin exhibits significant inhibitory effects on the proliferation of triple TNBC, both in vivo and in vitro. Moreover, it elicits an increase in lipid peroxide levels, downregulates the expression of ferroptosis-associated proteins GPX4 and xCT, and induces ferroptosis in breast cancer cells. Concurrently, Formononetin impedes the formation of the mammalian target of rapamycin complex 1 (mTORC1) and suppresses the expression of downstream Sterol regulatory element-binding protein 1(SREBP1). The utilization of breast cancer cells with SREBP1 overexpression or knockout demonstrates that Formononetin induces ferroptosis by modulating the mTORC1-SREBP1 signaling axis.

Discussion

In conclusion, this study provides evidence that Formononetin exerts an anti-proliferative effect on triple-negative breast cancer by inducing ferroptosis. Moreover, the mTORC1-SREBP1 signal axis is identified as the primary mechanism through which formononetin exerts its therapeutic effects. These findings suggest that formononetin holds promise as a potential targeted drug for clinical treatment of TNBC.
Improving the convergence of an iterative algorithm for solving arbitrary linear equation systems using classical or quantum binary optimization
Authors: Castro Erick R. , Martins Eldues O. , Sarthour Roberto S. , Souza Alexandre M. , Oliveira Ivan S.
Published: September 2024

Recent advancements in quantum computing and quantum-inspired algorithms have sparked renewed interest in binary optimization. These hardware and software innovations promise to revolutionize solution times for complex problems. In this work, we propose a novel method for solving linear systems. Our approach leverages binary optimization, making it particularly well-suited for problems with large condition numbers. We transform the linear system into a binary optimization problem, drawing inspiration from the geometry of the original problem and resembling the conjugate gradient method. This approach employs conjugate directions that significantly accelerate the algorithm’s convergence rate. Furthermore, we demonstrate that by leveraging partial knowledge of the problem’s intrinsic geometry, we can decompose the original problem into smaller, independent sub-problems. These sub-problems can be efficiently tackled using either quantum or classical solvers. Although determining the problem’s geometry introduces some additional computational cost, this investment is outweighed by the substantial performance gains compared to existing methods.
Structured illumination lensless digital holographic microscopy (SI-LDHM)
Authors: Zheng Juanjuan , Guo Xuhong , Ma Ying , Wen Kai , An Sha , Wang Xiaofang , Gao Peng , Qian Jiaming , Zuo Chao , Gao Peng
Published: September 2024

In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates a sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two copies of object waves along the ±1st diffraction orders of each SI. The reconstructed object waves under different illumination orientations are respectively propagated to the sample plane along the +1st diffraction order and then averaged, thus yielding a clean image without the artifact of twin images. Experimental results demonstrated that thanks to the multi-oriented SI strategy, the twin image in SI-LDHM is sevenfold reduced compared to conventional DHM, while the spatial resolution is 1.15 times higher.
High-throughput phenotyping reveals multiple drought responses of wild and cultivated Phaseolinae beans
Authors: Verheyen Jon , Dhondt Stijn , Abbeloos Rafael , Eeckhout Joris , Janssens Steven , Leyns Frederik , Scheldeman Xavier , Storme Veronique , Vandelook Filip
Published: September 2024

Introduction

Although drought resistance of a plant may be achieved through morphological, structural, physiological, cellular, and molecular adaptations, most studies remain limited to quantifying the effect of drought on biomass.

Methods

Using a highthroughput phenotypic imaging system, we evaluated the drought resistance of 151 bean accessions (Phaseolinae; Fabaceae) in an explorative approach, by quantifying five different traits simultaneously: biomass, water use efficiency (WUE), relative water content (RWC), chlorophyll content (NDVI), and root/shoot ratio. Since crop wild relatives are important resources for breeding programs, we analyzed both wild and cultivated accessions, most of which have never been evaluated for drought resistance before.

Results

We demonstrate that the five traits are affected very differently by drought in the studied accessions, with significant correlations existing only between the biomass and WUE indicators (r=0.39), and between the RWC and NDVI indicators (r=0.40). When grouping accessions by subgenus or by species, large intraspecific and withinsubgenus variation was found. For this reason, we performed a cluster analysis, which grouped the accessions into five distinct clusters with similar response profiles. We also correlated the drought resistance for each accession to local climate variables at their original collection sites. The biomass, WUE, and RWC indicators were significantly correlated to annual precipitation (r=0.40, r=0.20, r=0.22, respectively), confirming that accessions from arid environments are generally more drought resistant.

Discussion

Our results demonstrate that the drought resistance of Phaseolinae beans is a multifaceted characteristic and cannot be simply quantified through biomass. Furthermore, the broader knowledge of the drought resistance of the accessions studied here may prove an invaluable resource for future crop production.
Plant disease recognition datasets in the age of deep learning: challenges and opportunities
Authors: Xu Mingle , Park Ji-Eun , Lee Jaehwan , Yang Jucheng , Yoon Sook
Published: September 2024

Although plant disease recognition has witnessed a significant improvement with deep learning in recent years, a common observation is that current deep learning methods with decent performance tend to suffer in real-world applications. We argue that this illusion essentially comes from the fact that current plant disease recognition datasets cater to deep learning methods and are far from real scenarios. Mitigating this illusion fundamentally requires an interdisciplinary perspective from both plant disease and deep learning, and a core question arises. What are the characteristics of a desired dataset? This paper aims to provide a perspective on this question. First, we present a taxonomy to describe potential plant disease datasets, which provides a bridge between the two research fields. We then give several directions for making future datasets, such as creating challenge-oriented datasets. We believe that our paper will contribute to creating datasets that can help achieve the ultimate objective of deploying deep learning in real-world plant disease recognition applications. To facilitate the community, our project is publicly available at https://github.com/xml94/PPDRD with the information of relevant public datasets.
Evaluation and identification of advanced inter-specific derivatives from crosses of Cicer arietinum with C. reticulatum and C. echinospermum for agro-morphological, quality traits and disease resistance
Authors: Vadithya Amool Singh , Singh Inderjit , Rani Upasana , Venadan Sreya , Jajoriya Rajdeep , Singh Mohar , Oberoi Harpreet Kaur , Singh Sarvjeet , Bharadwaj Chellapilla , Bindra Shayla
Published: September 2024

Inter-specific hybridization is a key strategy in modern crop improvement, aiming to integrate desirable traits from wild species into cultivated backgrounds. This study delves into the evaluation and identification of advanced inter-specific derivatives (IDs) derived from crosses of cultivated chickpea with Cicer reticulatum and C. echinospermum. The primary aim was to incorporate desirable yield enhancement traits, disease resistance, and nutritional quality traits into cultivated chickpea. The IDs were assessed during rabi 2021-22 and 2022-23 in the northern plains zone of India. Significant amount of genetic variability was observed for key agro-morphological traits having high heritability and genetic advance. Superior derivatives were identified for early flowering, high seed yield, and resistance to Ascochyta blight, Botrytis grey mould, and Fusarium wilt. Significant variability for crude protein and total soluble sugar content was also observed among the derivatives. The findings highlight the potential of utilizing wild Cicer species to broaden the genetic base of cultivated chickpea for the development of robust, high-yielding, disease-resistant varieties with improved nutritional traits suitable for diverse environmental conditions. The superior derivatives identified in this study hold promise for future breeding programmes for improving productivity, disease resistance and nutritional quality.
Teacher support as predictors of Chinese EFL learners’ classroom flow: the mediating role of academic self-efficacy
Authors: Gong Wenting , Xu Chuang
Published: September 2024

Informed by social support theory and control-value theory, this study investigates the predictive role of teacher support on classroom flow among English as a Foreign Language (EFL) learners and the mediating effect of academic self-efficacy. A survey was conducted among 557 Chinese university EFL learners to gather relevant data. Descriptive statistics indicate that the participants exhibited a moderate level of classroom flow and exhibited significant variations based on gender and grade. Analysis using structural equation modeling revealed that teacher support has a noteworthy, positive predictive impact on EFL learners’ classroom flow. Furthermore, academic self-efficacy serves as a partial mediator between teacher support and classroom flow, with instrumental and emotional teacher support showing the strongest indirect effects. Notably, various dimensions of teacher support, aligning with students’ psychological needs as outlined by self-determination theory, have unique predictive effects on both classroom flow and academic self-efficacy. Appraisal and informational teacher support have the greatest predictive influence on classroom flow among the teacher support considered. The findings enhance understanding of the dynamics between teacher support, academic self-efficacy, and classroom flow, offering practical implications for creating autonomy-supportive educational environments that can elevate EFL learners’ engagement and academic achievements.
Antimicrobial stewardship situation analysis in selected hospitals in Zambia: findings and implications from a national survey
Authors: Chizimu Joseph Yamweka , Mudenda Steward , Yamba Kaunda , Lukwesa Chileshe , Chanda Raphael , Nakazwe Ruth , Simunyola Bwalya , Shawa Misheck , Kalungia Aubrey Chichonyi , Chanda Duncan , Chola Uchizi , Mateele Tebuho , Thapa Jeewan , Kapolowe Kenneth , Mazaba Mazyanga Lucy , Mpundu Mirfin , Masaninga Freddie , Azam Khalid , Nakajima Chie , Suzuki Yasuhiko , Bakyaita Nathan Nsubuga , Wesangula Evelyn , Matu Martin , Chilengi Roma
Published: September 2024

Background

Antimicrobial stewardship (AMS) programs are critical in combating antimicrobial resistance (AMR). In Zambia, there is little information regarding the capacity of hospitals to establish and implement AMS programs. The objective of this study was to conduct a baseline assessment of WHO core elements for an AMS program implementation in eight hospitals in Zambia.

Materials and methods

We conducted an exploratory cross-sectional study from September 2023 to December 2023 using a self-scoring Periodic National and Healthcare Facility Assessment Tool from the World Health Organization (WHO) policy guidance on integrated AMS activities in human health. Eight public hospitals were surveyed across the five provinces of Zambia. Data was analyzed using the WHO self-scoring tool and thematic analysis.

Results

Overall, 62.5% (6/8) of the facilities scored low (below 60%) in implementing AMS programs. Most facilities had challenges with reporting AMS feedback within the hospital (average score = 46%), Drugs and Therapeutics Committee (DTC) functionality (average score = 49%), AMS actions (average score = 50%), education and training (average score = 54%), and leadership commitment to AMS activities (average score = 56%). The overall score for all AMS core elements was average (56%). All the hospitals (100%) did not have an allocated budget for AMS programs. Finally, there were neither antibiograms to guide antimicrobial utilization nor AMS-trained staff in more than 50% of the hospitals surveyed.

Conclusion

This study found low AMS implementation in these public hospitals, especially where DTCs were non-functional. The identified challenges and gaps require urgent attention for sustainable multidisciplinary AMS programs.
The association between hobby engagement and depressive symptoms among Chinese middle-aged and older adults: evidence from the China health and retirement longitudinal study
Authors: Lu Qiong , Hu Peijing , Lian Cheng , Chen Xinglin
Published: September 2024

Background

It has been demonstrated that receptive cultural engagement, such as visits to theaters and museums, can reduce depression in older adults. However, the association between hobby engagement and lower rates of depressive symptoms (DS) remains unclear. The objective of this study was to investigate the potential relationship between hobby engagement and depressive symptoms.

Methods

The data for this prospective cross-sectional study were collected from participants in the China Longitudinal Study of Health and Retirement (CHARLS) wave 2020. To assess DS, a score of 10 or more on the Center for Epidemiological Studies Depression Scale was used to indicate depression. Hobby engagement was gaged by trained staff through the administration of a battery of standardized questions. Logistic regression and inverse probability of treatment weighting (IPTW) using the propensity score analyses were employed to investigate the relationship between hobby engagement and DS.

Results

A total of 16,057 participants were included, with a mean age of 62.4 ± 9.2 years. Of these, 7,699 were male and 8,358 were female. The proportion of individuals exhibiting depressive symptoms was 31.57% (1,286 out of 4,073) among those with hobby engagement, while it was 39.67% (4,754 out of 11,984) among those without hobby engagement. After adjusting for covariates, the odds ratio was 0.89 (95% CI: 0.82–0.97, p = 0.0109). Propensity-score analyses also supported these findings, with the odds ratio being 0.91 (95% CI: 0.84–0.99, p = 0.0204). The finding was consistent with multiple sensitivity analyses.

Conclusion

Our study found hobby engagement was associated with a reduced risk of depressive symptoms in Chinese middle-aged and older adults. Our findings need to be confirmed in future studies.
Acceptance and commitment therapy group protocol for caregivers of anxious youth: an open trial pilot study
Authors: Raftery-Helmer Jacquelyn N. , Hart Ashley S. , Levitt Madeline R. , Hodge Steven M. , Coyne Lisa W. , Moore Phoebe S.
Published: September 2024

Introduction

Anxiety disorders are common, distressing, and impairing for children and families. Cognitive-behavioral interventions targeting the role of family interactions in child anxiety treatment may be limited by lack of attention to antecedents to parental control; specifically, internal parent factors such as experiential avoidance and cognitive fusion. This pilot study evaluates the preliminary efficacy of a group-delivered caregiver treatment program, ACT for Parents of Anxious Children (ACT-PAC) that targets parental experiential avoidance, cognitive fusion, and child internalizing symptoms.

Methods

Twenty-three youth ages 7–17 years with a primary anxiety disorder diagnosis and their primary caregiver participated in six one-hour, weekly group treatment sessions. Parents and children reported on child symptomatology and parents reported on parent symptomatology and quality of life at two assessment points: within one week before ACT-PAC treatment and within one week after treatment. Parents self-reported on parental internal processes specifically targeted by ACT (e.g., cognitive fusion) weekly during the 6-week treatment.

Results

Results support the feasibility and acceptability of ACT-PAC and indicate reductions in parents' cognitive fusion and child internalizing symptoms.
Effects of oral liquiritigenin inoculation on gut microbiota and gene expression in intestinal and extraintestinal tissues of mice
Authors: Suo Zhaotaize , Yu Ying , Shi Fangyun , Tian Jijing , Hao Zhihui , Zhang Jingren , Zou Jun
Published: September 2024

Introduction

Liquiritigenin (LQ), a natural flavonoid found in traditional Chinese medicine and often administered orally, holds potential to affect both the gut and its microbiota, that potentially mediating or influencing its biological and pharmacological effects. However, the effects of LQ on gut microbiota composition and intestinal function remain poorly understood. In this study, we aimed to explore the impact of LQ on gut microbiota and gene expression in both intestinal and extraintestinal tissues.

Methods

We orally inoculated six-week-old SPF C57BL/6 mice with either LQ (a concentration of 4 mg/ml diluted in dimethylsulfoxide, (DMSO)) or DMSO, and administered daily for a duration of 2 weeks. At the end of the experimental period, all mice were euthanized. Fresh fecal samples, as well as samples from the intestine, lung, and liver, were collected for subsequent microbiota analysis, RNA-seq, or histochemical and immunohistochemical (IHC) staining.

Results

Findings show that LQ alters gut microbiota composition, enhancing microbial correlations in the colon but causing some dysbiosis, evidenced by increased pathobionts, decreased beneficial bifidobacteria, and reduced microbiota diversity. Gene expression analysis reveals LQ upregulates mucosal immune response genes and antiinfection genes in both the intestine and lung, with histology confirming increased Paneth cells and antimicrobial peptides in the intestine. Additionally, LQ affects tissue-specific gene expression, triggering hypersensitivity genes in the colon, downregulating metabolic genes in the small intestine, and reducing cell motility and adhesion genes in the lung.

Discussion

These results suggest LQ’s potential to modulate common mucosal immunity but also highlight possible risks of gut dysbiosis and hypersensitivity, particularly in vulnerable individuals. Our study, while informative about the effects of LQ on gut health, lacks direct evidence on whether changes in gut microbiota and gene expression caused by LQ impact inflammatory diseases or are causally linked. Future research should investigate this through fecal microbiota transplantation to explore the causal relationships and LQ’s potential effects on immune responses and disease outcomes in relevant models.
Relationship between the contact load and time-loss injuries in rugby union
Authors: Iwasaki Yusuke , Someya Yuki , Nagao Masashi , Nozu Shojiro , Shiota Yuki , Takazawa Yuji
Published: September 2024

Objective

Quantifying and managing the matches and training loads of players is important for injury prevention. As rugby union is a full-contact sport and frequent contact injuries occur, it might also be important to quantify and manage players’ contact loads. This study aimed to clarify the relationship between contact load and injury incidence in elite rugby union players.

Methods

Forty-eight elite rugby union players (27.0 ± 3.5 years) in Japan were monitored during one season (8 months). The contact load, an index of training load, was evaluated as collision count and collision load measured using a global positioning system device, and then calculated using the acute:chronic workload ratio (ACWR) based on the exponentially weighted moving average (EWMA). The association between the EWMA-ACWR of contact load and injury incidence was analyzed using generalized estimating equations.

Results

Of the 58 injuries during one season, 70.7% were contact injuries. Collision counts and collision load calculated by EWMA-ACWR were associated with the risk of injury (p < 0.01 both), with the odds ratios were 4.20 [95% confidence interval (CI): 1.74–10.11] and 4.44 (95% CI: 1.95–10.13), respectively.

Conclusion

Contact load calculated using EWMA-ACWR was associated with injury in elite rugby union players.
Molecular characterization of cDNA coding for 33.5 and 41 kDa oocyst and sporocyst proteins that are differentially regulated in different strains of Eimeria maxima
Authors: Jenkins Mark C. , Parker Carolyn , Jansen Andrew , Papadopoulos Marianne Dias , Tucker Matthew S.
Published: September 2024

Eimeria maxima (APU1 and APU2) differ in virulence for chickens, due in part to the greater fecundity of the former. In a previous study, RNA-seq was used to identify a transcripts upregulated in E. maxima APU1 compared to E. maxima APU2. In this study, 2 of these upregulated genes (EMWEY 23530 and EMWEY 48910) were characterized by first confirming upregulation using quantitative RT-PCR. For both EMWEY 23530 and EMWEY 48910, RNA transcription was fairly consistent during sporulation. The extent of differential expression was about 2-fold log2 higher in APU-1 compared to APU-2 (peaking at 18 h for EMWEY 23530 and 0 h for EMWEY 48910). EMWEY 23530 and EMWEY 48910 cDNA were cloned and expressed as polyHis-fusion proteins in Escherichia coli. The observed size of recombinant EMWEY 23530 was 24 kDa; the observed size of recombinant EMWEY 48910 was 35 kDa, which are consistent with the predicted size based on the coding sequences. Immunostaining 2D gel blots of E. maxima APU1 and APU2 oocyst/sporocyst protein with antisera specific for EMWEY 23530 identified a 33.5 kDa protein with a pH 7.4 isoelectric point (Emax p33.5). Similar 2D gel blot analysis with EMWEY 48910 identified a 41 kDa protein with a pH 7.2 isoelectric point (Emax p41). The intensity of Emax p33.5 and Emax p41 was noticeably greater in oocyst/sporocyst proteins from E. maxima APU1 compared to E. maxima APU2. This was corroborated by ELISA wherein equal amounts of total E. maxima APU1 and APU2 protein were probed with serial dilutions of anti-rEmax p33.5 or anti-rEmax p41. Immunofluorescence (IFA) staining of permeabilized unsporulated E. maxima APU1 and APU2 oocysts revealed Emax p33.5 to be localized in one end of oocysts, while Emax p41 appeared on the surface of oocysts. After sporulation, the p33.5 and p41 antigens appeared loosely associated with sporocysts. Taken together, these data confirm excess expression of two proteins in the E. maxima strain characterized by greater fecundity and virulence, and may provide insight into basis for phenotypic differences among different E. maxima.
Intelligent Detection of Underwater Defects in Concrete Dams Based on YOLOv8s-UEC
Authors: Liang Chenxi , Zhao Yang , Kang Fei
Published: September 2024

This study proposes a concrete dam underwater apparent defect detection algorithm named YOLOv8s-UEC for intelligent identification of underwater defects. Due to the scarcity of existing images of underwater concrete defects, this study establishes a dataset of underwater defect images by manually constructing defective concrete walls for the training of defect detection networks. For the defect feature ambiguity that exists in underwater defects, the ConvNeXt Block module and Efficient-RepGFPN structure are introduced to enhance the feature extraction capability of the network, and the P2 detection layer is fused to enhance the detection capability of small-size defects such as cracks. The results show that the mean average precision (mAP0.5 and mAP0.5:0.95) of the improved algorithm are increased by 1.4% and 5.8%, and it exhibits good robustness and considerable detection effect for underwater defects.
The Rumen and Gastrointestinal Microbial Environment and Its Association with Feed Efficiency and Pregnancy in Female Beef Cattle
Authors: Martin M. Gabbi , Cordero-Llarena Juan F. , Voy Brynn H. , McLean Kyle J. , Myer Phillip R.
Published: September 2024

With the anticipated population growth by 2050, the demand for high-quality protein for human consumption is set to rise. To enhance the sustainability of U.S. cattle production, producers and researchers have traditionally concentrated on improving the feed efficiency of steers through advancements in genetics, nutrition, and microbiome tools, resulting in a more marketable beef product. However, without successful pregnancies, there would be no marketable animals to feed. Despite extensive research on hormonal impacts on cattle physiology, including nutrition and reproduction, there is limited knowledge about how the rumen microbial environment is impacted by pregnancy and feed efficiency in female beef cattle. Understanding the rumen microbiome’s role in feed efficiency and its response to hormonal changes during pregnancy is crucial for advancing sustainable beef cattle production. Therefore, this review highlights the importance of understanding the microbial dynamics in the rumen of pregnant beef females. Although progress has been made, gaps remain in understanding how varying nutritional requirements throughout pregnancy affect the rumen microbiome, highlighting the need for continued research. Addressing these areas will lead to more efficient and sustainable cattle production practices, benefiting beef production and contributing to global food security.
Preparation and Characterization of Hydroxylated Recombinant Collagen by Incorporating Proline and Hydroxyproline in Proline-Deficient Escherichia coli
Authors: Cheng Zhimin , Hong Bin , Li Yanmei , Wang Jufang
Published: September 2024

Collagen possesses distinctive chemical properties and biological functions due to its unique triple helix structure. However, recombinant collagen expressed in Escherichia coli without post-translational modifications such as hydroxylation lacks full function since hydroxylation is considered to be critical to the stability of the collagen triple-helix at body temperature. Here, a proline-deficient E. coli strain was constructed and employed to prepare hydroxylated recombinant collagens by incorporating proline (Pro) and hydroxyproline (Hyp) from the culture medium. By controlling the ratio of Pro to Hyp in the culture medium, collagen with different degrees of hydroxylation (0–88%) can be obtained. When the ratio of Pro and Hyp was adjusted to 12:8 mM, the proline hydroxylation rate of recombinant human collagen (rhCol, 55 kDa) ranged from 40–50%, which was also the degree of natural collagen. After proline hydroxylation, both the thermal stability and cell binding of rhCol were significantly enhanced. Notably, when the hydroxylation rate approached that of native human collagen (40–50%), the improvements were most pronounced. Moreover, the cell binding of rhCol with a hydroxylation rate of 43% increased by 29%, and the melting temperature (Tm) rose by 5 °C compared to the non-hydroxylated rhCol. The system achieved a yield of 1.186 g/L of rhCol by batch-fed in a 7 L fermenter. This innovative technology is expected to drive the development and application of collagen-related biomaterials with significant application value in the fields of tissue engineering, regenerative medicine, and biopharmaceuticals.
Adipose Tissue Plasticity: A Comprehensive Definition and Multidimensional Insight
Authors: Mo Yu-Yao , Han Yu-Xin , Xu Shi-Na , Jiang Hong-Li , Wu Hui-Xuan , Cai Jun-Min , Li Long , Bu Yan-Hong , Xiao Fen , Liang Han-Dan , Wen Ying , Liu Yu-Ze , Yin Yu-Long , Zhou Hou-De
Published: September 2024

Adipose tissue is composed of adipocytes, stromal vascular fraction, nerves, surrounding immune cells, and the extracellular matrix. Under various physiological or pathological conditions, adipose tissue shifts cellular composition, lipid storage, and organelle dynamics to respond to the stress; this remodeling is called “adipose tissue plasticity”. Adipose tissue plasticity includes changes in the size, species, number, lipid storage capacity, and differentiation function of adipocytes, as well as alterations in the distribution and cellular composition of adipose tissue. This plasticity has a major role in growth, obesity, organismal protection, and internal environmental homeostasis. Moreover, certain thresholds exist for this plasticity with significant individualized differences. Here, we comprehensively elaborate on the specific connotation of adipose tissue plasticity and the relationship between this plasticity and the development of many diseases. Meanwhile, we summarize possible strategies for treating obesity in response to adipose tissue plasticity, intending to provide new insights into the dynamic changes in adipose tissue and contribute new ideas to relevant clinical problems.
Advancements in Pharmacological Interventions and Novel Therapeutic Approaches for Amyotrophic Lateral Sclerosis
Authors: Montiel-Troya María , Mohamed-Mohamed Himan , Pardo-Moreno Teresa , González-Díaz Ana , Ruger-Navarrete Azahara , de la Mata Fernández Mario , Tovar-Gálvez María Isabel , Ramos-Rodríguez Juan José , García-Morales Victoria
Published: September 2024

(1) Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease in which the patient suffers from an affection of both upper and lower motor neurons at the spinal and brainstem level, causing a progressive paralysis that leads to the patient’s demise. Gender is also considered a predisposing risk factor for developing the disease. A brief review of the pathophysiological mechanisms of the disease is also described in this work. Despite the fact that a cure for ALS is currently unknown, there exists a variety of pharmacological and non-pharmacological therapies that can help reduce the progression of the disease over a certain period of time and alleviate symptoms. (2) We aim to analyze these pharmacological and non-pharmacological therapies through a systematic review. A comprehensive, multidisciplinary approach to treatment is necessary. (3) Drugs such as riluzole, edaravone, and sodium phenylbutyrate, among others, have been investigated. Additionally, it is important to stay updated on research on new drugs, such as masitinib, from which very good results have been obtained. (4) Therapies aimed at psychological support, speech and language, and physical therapy for the patient are also available, which increase the quality of life of the patients.
Chronic Kidney Disease Progression—A Challenge
Authors: Maringhini Silvio , Zoccali Carmine
Published: September 2024

Chronic kidney disease (CKD) is a progressive condition characterized by a continuous decline in renal function, independent of the initial cause of damage or external factors such as infection, inflammation, or toxins. The accurate measurement of renal function, typically assessed using the glomerular filtration rate (GFR), is crucial for managing CKD. The most accepted hypothesis for CKD progression is glomerular damage caused by hyperfiltration. Various factors can accelerate CKD progression, and several biomarkers have been identified to monitor this progression. Numerous studies have explored the risk factors associated with CKD progression, and some of these factors can be modified. Additionally, several drugs are now available that can reduce CKD progression. This review summarizes recent publications and highlights potential future research directions in CKD progression. It discusses the evolution of GFR measurement methods, the mechanisms driving CKD progression, and the latest findings on biomarkers and risk factors. Furthermore, it explores therapeutic strategies, including dietary modifications and pharmacological interventions, to slow CKD progression. Understanding these mechanisms and interventions is crucial for developing effective therapeutic strategies to prevent or slow CKD progression.
Exploring Liraglutide in Lithium–Pilocarpine-Induced Temporal Lobe Epilepsy Model in Rats: Impact on Inflammation, Mitochondrial Function, and Behavior
Authors: Antmen Fatma Merve , Fedaioglu Zeynep , Acar Dilan , Sayar Ahmed Kerem , Yavuz Ilayda Esma , Ada Ece , Karakose Bengisu , Rzayeva Lale , Demircan Sevcan , Kardouh Farah , Senay Simge , Kolgazi Meltem , Suyen Guldal , Oz-Arslan Devrim
Published: September 2024

Background/Objectives: Glucagon-like peptide-1 receptor agonists such as liraglutide are known for their neuroprotective effects in neurodegenerative disorders, but their role in temporal lobe epilepsy (TLE) remains unclear. We aimed to investigate the effects of liraglutide on several biological processes, including inflammation, antioxidant defense mechanisms, mitochondrial dynamics, and function, as well as cognitive and behavioral changes in the TLE model. Methods: Low-dose, repeated intraperitoneal injections of lithium chloride–pilocarpine hydrochloride were used to induce status epilepticus (SE) in order to develop TLE in rats. Fifty-six male Sprague Dawley rats were subjected and allocated to the groups. The effects of liraglutide on inflammatory markers (NLRP3, Caspase-1, and IL-1β), antioxidant pathways (Nrf-2 and p-Nrf-2), and mitochondrial dynamics proteins (Pink1, Mfn2, and Drp1) were evaluated in hippocampal tissues via a Western blot. Mitochondrial function in peripheral blood mononuclear cells (PBMCs) was examined using flow cytometry. Cognitive-behavioral outcomes were assessed using the open-field, elevated plus maze, and Morris water maze tests. Results: Our results showed that liraglutide modulates NLRP3-mediated inflammation, reduces oxidative stress, and triggers antioxidative pathways through Nrf2 in SE-induced rats. Moreover, liraglutide treatment restored Pink1, Mfn2, and Drp1 levels in SE-induced rats. Liraglutide treatment also altered the mitochondrial function of PBMCs in both healthy and epileptic rats. This suggests that treatment can modulate mitochondrial dynamics and functions in the brain and periphery. Furthermore, in the behavioral aspect, liraglutide reversed the movement-enhancing effect of epilepsy. Conclusions: This research underscores the potential of GLP-1RAs as a possibly promising therapeutic strategy for TLE.
Bacterial and Viral Co-Infections in COVID-19 Patients: Etiology and Clinical Impact
Authors: Trifonova Ivelina , Madzharova Iveta , Korsun Neli , Levterova Viktoria , Velikov Petar , Voleva Silvya , Ivanov Ivan , Ivanov Daniel , Yordanova Ralitsa , Tcherveniakova Tatiana , Angelova Svetla , Christova Iva
Published: September 2024

Background: Mixed infections can worsen disease symptoms. This study investigated the impact of mixed infections with viral and bacterial pathogens in patients positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Using the in-house multiplex PCR method, we tested 337 SARS-CoV-2 positive samples for co-infections with three bacterial and 14 other viral pathogens. Results: Between August 2021 and May 2022, 8% of 337 SARS-CoV-2-positive patients had bacterial co-infections, 5.6% had viral co-infections, and 1.4% had triple mixed infections. The most common causes of mixed infections were Haemophilus influenzae (5.93%) and respiratory syncytial virus (RSV) (1.18%). Children < 5 years old had more frequent co-infections than adults < 65 years old (20.8% vs. 16.4%), while adults showed a more severe clinical picture with a higher C-reactive protein (CRP) level (78.1 vs.16.2 mg/L; p = 0.033), a lower oxygen saturation (SpO2) (89.5 vs. 93.2%), and a longer hospital stay (8.1 vs. 3.1 days; p = 0.025) (mean levels). The risk of a fatal outcome was 41% in unvaccinated patients (p = 0.713), which increased by 2.66% with co-infection with two pathogens (p = 0.342) and by 26% with three pathogens (p = 0.005). Additionally, 50% of intensive care unit (ICU) patients had a triple infection, compared with only 1.3% in the inpatient unit (p = 0.0029). The risk of death and/or ICU admission was 12 times higher (p = 0.042) with an additional pathogen and increased by 95% (p = 0.003) with a third concomitant pathogen. Conclusions: Regular multiplex testing is important for prompt treatment and targeted antibiotic use.
Selective Laser Trabeculoplasty after a Previous Glaucoma Treatment
Authors: Thelen Patrick , Böhringer Daniel , Keye Philip , Reinhard Thomas , Lübke Jan
Published: September 2024

Background/Objectives: Recent prospective studies have shown that selective laser tra-beculoplasty (SLT) is a safe and cost-effective alternative to pressure-reducing eye drop therapy as a first-line treatment for ocular hypertension or open-angle glaucoma. In addition to its comparable efficacy to eye drop therapy, SLT has been particularly effective in delaying the time until a surgical intervention is needed. The aim of our evaluation is to analyze patients who have received SLT following a pressure-reducing procedure. The primary endpoint is the duration until a subsequent interventional or surgical procedure is required. Methods: A retrospective analysis of 98 patients who underwent selective laser trabeculoplasty following a previous pressure-reducing procedure between 2017 and 2023. The statistical analyses included Cox regression and Kaplan–Meier survival estimations. Results: In total, 122 eyes of 98 patients received selective laser trabeculoplasty following a previous pressure-reducing procedure at the Department of Ophthalmology in Freiburg. The median follow-up period was 381.5 days (range 43.25–862.75 days). Approximately 68% of the eyes did not require another pressure-reducing procedure 365 days after the intervention, while about 58% of the eyes remained without another procedure after 730 days, according to Kaplan–Meier analysis. No significant difference was found between the different types of glaucoma regarding the duration until a subsequent pressure-reducing procedure was needed. The study indicated a tendency for patients with pseudoexfoliation glaucoma to undergo a secondary intervention earlier compared to those with primary open-angle glaucoma (p = 0.16). The intraocular pressure before SLT had a significant impact on the duration until the subsequent operation (p = 0.005). Conclusions: SLT is an effective method even after a previous pressure-reducing procedure for patients in whom further pressure-reducing interventions need to be delayed.
Leveraging Machine Learning for Optimized Mechanical Properties and 3D Printing of PLA/cHAP for Bone Implant
Authors: Omigbodun Francis T. , Osa-Uwagboe Norman , Udu Amadi Gabriel , Oladapo Bankole I.
Published: September 2024

This study explores the fabrication and characterisation of 3D-printed polylactic acid (PLA) scaffolds reinforced with calcium hydroxyapatite (cHAP) for bone tissue engineering applications. By varying the cHAP content, we aimed to enhance PLA scaffolds’ mechanical and thermal properties, making them suitable for load-bearing biomedical applications. The results indicate that increasing cHAP content improves the tensile and compressive strength of the scaffolds, although it also increases brittleness. Notably, incorporating cHAP at 7.5% and 10% significantly enhances thermal stability and mechanical performance, with properties comparable to or exceeding those of human cancellous bone. Furthermore, this study integrates machine learning techniques to predict the mechanical properties of these composites, employing algorithms such as XGBoost and AdaBoost. The models demonstrated high predictive accuracy, with R2 scores of 0.9173 and 0.8772 for compressive and tensile strength, respectively. These findings highlight the potential of using data-driven approaches to optimise material properties autonomously, offering significant implications for developing custom-tailored scaffolds in bone tissue engineering and regenerative medicine. The study underscores the promise of PLA/cHAP composites as viable candidates for advanced biomedical applications, particularly in creating patient-specific implants with improved mechanical and thermal characteristics.
A Simple Methodology to Gain Insights into the Physical and Compositional Features of Ternary and Quaternary Compounds Based on the Weight Percentages of Their Constituent Elements: A Proof of Principle Using Conventional EDX Characterizations
Authors: Garrido-García Luis Fernando , Pérez-Martínez Ana Laura , Reyes-Gasga José , Aguilar-Del-Valle María del Pilar , Wong Yew Hoong , Rodríguez-Gómez Arturo
Published: September 2024

Ternary and quaternary compounds offer vast potential for tailoring material properties through compositional adjustments and complex interactions among their constituent elements. However, many of their compositional possibilities still need to be investigated. Energy-dispersive X-ray spectroscopy (EDX) is crucial for determining elemental composition but is inadequate for identifying chemical bonds and physical properties. This work introduces a novel methodology using a stoichiometric deviation vector (SDV) to estimate the physical and compositional feature characteristics of Si, N, and O compounds by comparing actual molar ratios with ideal stoichiometric references. We validated this method by estimating Si-O bonds in silicon oxynitride samples, demonstrating strong agreement with FTIR and refractive index results. We also extended our proof of principle for SiAlON compounds and established an adaptable procedure to analyze compounds with more than three elements. This flexible methodology will significantly value the materials research community, providing valuable compositional features and physical insights by performing elemental EDX characterizations.