bluuebunny/crossref_metadata_2025 huggingface dataset:
Beyond Housing: Perceptions of Indirect Displacement, Displacement Risk, and Aging Precarity as Challenges to Aging in Place in Gentrifying Cities
Authors: Versey H. Shellae , Murad Serene , Willems Paul , Sanni Mubarak
Published: November 2019

Neighborhoods within age-friendly cities and communities are an important factor in shaping the everyday lives of older adults. Yet, less is known about how neighborhoods experiencing change influence the ability to age in place. One type of rapid neighborhood change occurring across major cities nationally and globally is gentrification, a process whereby the culture of an existing neighborhood changes through the influx of more affluent residents and businesses. Few studies have considered the impact of gentrification on older adults, who are among the most vulnerable to economic and social pressures that often accompany gentrification. The current study explores one consequence of gentrification, indirect displacement. While gentrification-induced displacement can refer to the physical (e.g., direct) displacement of residents moving out of a neighborhood due to rising housing costs, it also references the replacement of the unique character and social identity of a neighborhood (e.g., indirect displacement). We examine perceptions of the latter, characterized by perceived cultural shifts and housing concerns among adults aging in place in a gentrifying neighborhood in New York City. The implications of indirect displacement for displacement risk and aging precarity are discussed as potential threats to aging in place in age-friendly cities.
Are the Levels of Lipid Parameters Associated with Biometeorological Conditions?
Authors: Skutecki Rafał , Cymes Iwona , Dragańska Ewa , Glińska-Lewczuk Katarzyna , Buciński Adam , Drozdowski Marek , Romaszko Jerzy
Published: November 2019

Lipid disorders, especially hypercholesterolemia, are one of the most thoroughly investigated cardiovascular risk factors. Their correlation with biometeorological conditions has been reported, with authors stressing seasonal increases of total cholesterol (TC) levels, mostly occurring in winter. This study aims at determining the correlation between the level of lipid parameters (LP) and meteorological conditions, analyzing seasonal variations in LP levels, and attempting to answer the following questions: do changes in LP levels result from the organism’s response to cold or heat stress, or are they secondary to seasonal dietary variations? An observational study comprised ambulatory patients from the city of Olsztyn (Poland), for whom laboratory test were performed in 2016–2018, with 106,325 records of TC, high-density lipoprotein (HDL), and triglycerides (TG). LP levels were matched with atmospheric conditions on the day when the test was conducted and expressed by the universal thermal climate index (UTCI). We demonstrated seasonal increases of TC in cold stress (in wintertime) and of TG in heat stress (summer). The analysis of LP levels in specific periods revealed the increase of TC levels after holidays (i.e., Christmas and Easter) in men by 4.56%, and the increase of TG levels in women by 13.46% in the same period. Our results suggest the secondary, diet-dependent underlying cause of the observed changes. This work contributes to the discussion concerning the impact of biometeorological factors on LP levels and may be of significance when planning population-dedicated preventive activities.
The Role of Health Literacy in Explaining the Relation between Educational Level and Decision Making about Colorectal Cancer Screening
Authors: Woudstra Anke J. , Smets Ellen M. A. , Verdam Mathilde G. E. , Fransen Mirjam P.
Published: November 2019

Little is known about why educational inequalities exist in informed decision making in colorectal cancer (CRC) screening. Insight into the role and impact of health literacy is essential for intervention development. This study investigates associations between health literacy and informed decision making in CRC screening and explores to what extent health literacy mediates the association between education and informed decision making in CRC screening. In total, 696 individuals eligible for CRC screening (55–75 years of age) were recruited from online panels and filled in an online questionnaire at T0 (n = 696), T1 (n = 407) and T2 (n = 327). A hypothetical mediation model was tested using structural equation modelling. Outcomes included CRC knowledge, CRC screening knowledge, attitude, injunctive norm, descriptive norm, risk perception, self-efficacy, decisional conflict and decisional certainty. Health literacy domains included Comprehension, Application, Numeracy and Communication. Comprehension, Application and Numeracy, were found to mediate the association between education and knowledge about CRC and CRC screening, injunctive norm, descriptive norm, decisional conflict and decisional certainty. In light of these findings, targeting multiple health literacy domains in decision-support interventions is essential for facilitating informed decision making in CRC screening.
Relationship Between Early Childhood Non-Parental Childcare and Diet, Physical Activity, Sedentary Behaviour, and Sleep: A Systematic Review of Longitudinal Studies
Authors: Costa Silvia , Benjamin-Neelon Sara E. , Winpenny Eleanor , Phillips Veronica , Adams Jean
Published: November 2019

The rising prevalence of childhood obesity is a global public health concern. Evidence suggests that exposure to non-parental childcare before age six years is associated with development of obesity, diet, and activity behaviours (physical activity, sedentary behaviour, and sleep). However, findings are inconsistent and mostly from cross-sectional studies, making it difficult to identify the direction of causation in associations. This review identified and synthesised the published research on longitudinal associations between non-parental childcare during early childhood, diet, and activity behaviours. Seven databases were searched, and results were independently double-screened through title/abstract and full-text stages. Included studies were evaluated for risk of bias. Of the 18,793 references screened, 13 met eligibility criteria and were included in the review. These presented results on 89 tested childcare/outcome associations, 63 testing diet outcomes (59% null, remainder mixed), and 26 testing activity behaviour outcomes (85% null, remainder mixed). The scarce available literature indicates little and mixed evidence of a longitudinal association. This reflects a paucity of research, rather than clear evidence of no effect. There is an urgent need for studies investigating the longitudinal associations of non-parental childcare on diet and activity behaviours to assess potential lasting effects and mechanisms; whether and how effects vary by provider; and differences by intensity, duration, and population sub-groups.
Cardiometabolic Risk Factors and Physical Activity Patterns Maximizing Fitness and Minimizing Fatness Variation in Malaysian Adolescents: A Novel Application of Reduced Rank Regression
Authors: Toumpakari Zoi , Jago Russell , Howe Laura D. , Majid Hazreen Abdul , Papadaki Angeliki , Mohammadi Shooka , Jalaludin Muhammad Yazid , Dahlui Maznah , Nahar Azmi Mohamed Mohd , Su Tin Tin , Johnson Laura
Published: November 2019

Patterns of physical activity (PA) that optimize both fitness and fatness may better predict cardiometabolic health. Reduced rank regression (RRR) was applied to identify combinations of the type (e.g., football vs. skipping), location and timing of activity, explaining variation in cardiorespiratory fitness (CRF) and Body Mass Index (BMI). Multivariable regressions estimated longitudinal associations of PA pattern scores with cardiometabolic health in n = 579 adolescents aged 13–17 years from the Malaysian Health and Adolescent Longitudinal Research Team study. PA pattern scores in boys were associated with higher fitness (r = 0.3) and lower fatness (r = −0.3); however, in girls, pattern scores were only associated with higher fitness (r = 0.4) (fatness, r = −0.1). Pattern scores changed by β = −0.01 (95% confidence interval (CI) −0.04, 0.03) and β = −0.08 (95% CI −0.1, −0.06) per year from 13 to 17 years in boys and girls respectively. Higher CRF and lower BMI were associated with better cardiometabolic health at 17 years, but PA pattern scores were not in either cross-sectional or longitudinal models. RRR identified sex-specific PA patterns associated with fitness and fatness but the total variation they explained was small. PA pattern scores changed little through adolescence, which may explain the limited evidence on health associations. Objective PA measurement may improve RRR for identifying optimal PA patterns for cardiometabolic health.
The Multifaceted Roles of Plant Hormone Salicylic Acid in Endoplasmic Reticulum Stress and Unfolded Protein Response
Authors: Poór Péter , Czékus Zalán , Tari Irma , Ördög Attila
Published: November 2019

Different abiotic and biotic stresses lead to the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum (ER), resulting in ER stress. In response to ER stress, cells activate various cytoprotective responses, enhancing chaperon synthesis, protein folding capacity, and degradation of misfolded proteins. These responses of plants are called the unfolded protein response (UPR). ER stress signaling and UPR can be regulated by salicylic acid (SA), but the mode of its action is not known in full detail. In this review, the current knowledge on the multifaceted role of SA in ER stress and UPR is summarized in model plants and crops to gain a better understanding of SA-regulated processes at the physiological, biochemical, and molecular levels.
Trypanosoma brucei J-Protein 2 Functionally Co-Operates with the Cytosolic Hsp70 and Hsp70.4 Proteins
Authors: Bentley Stephen John , Boshoff Aileen
Published: November 2019

The etiological agent of African trypanosomiasis, Trypanosoma brucei (Tb), has been identified to possess an expanded and diverse group of heat shock proteins, which have been implicated in cytoprotection, differentiation, and subsequently progression and transmission of the disease. Heat shock protein 70 (Hsp70) is a highly conserved and ubiquitous molecular chaperone that is important in maintaining protein homeostasis in the cell. Its function is regulated by a wide range of co-chaperones, and inhibition of these functions and interactions with co-chaperones are emerging as potential therapeutic targets for numerous diseases. This study sought to biochemically characterize the cytosolic TbHsp70 and TbHsp70.4 proteins and to investigate if they functionally co-operate with the Type I J-protein, Tbj2. Expression of TbHsp70 was shown to be heat inducible, while TbHsp70.4 was constitutively expressed. The basal ATPase activities of TbHsp70.4 and TbHsp70 were stimulated by Tbj2. It was further determined that Tbj2 functionally co-operated with TbHsp70 and TbHsp70.4 as the J-protein was shown to stimulate the ability of both proteins to mediate the refolding of chemically denatured β-galactosidase. This study provides further insight into this important class of proteins, which may contribute to the development of new therapeutic strategies to combat African Trypanosomiasis.
Strategies to Overcome Antimicrobial Resistance (AMR) Making Use of Non-Essential Target Inhibitors: A Review
Authors: Annunziato Giannamaria
Published: November 2019

Antibiotics have always been considered as one of the most relevant discoveries of the twentieth century. Unfortunately, the dawn of the antibiotic era has sadly corresponded to the rise of the phenomenon of antimicrobial resistance (AMR), which is a natural process whereby microbes evolve in such a way to withstand the action of drugs. In this context, the identification of new potential antimicrobial targets and/or the identification of new chemical entities as antimicrobial drugs are in great demand. To date, among the many possible approaches used to deal with antibiotic resistance is the use of antibiotic adjuvants that hit bacterial non-essential targets. In this review, the author focuses on the discovery of antibiotic adjuvants and on new tools to study and reduce the prevalence of resistant bacterial infections.
Ceftriaxone Treatment Affects EAAT2 Expression and Glutamatergic Neurotransmission and Exerts a Weak Anticonvulsant Effect in Young Rats
Authors: Zaitsev Aleksey V. , Malkin Sergey L. , Postnikova Tatyana Y. , Smolensky Ilya V. , Zubareva Olga E. , Romanova Irina V. , Zakharova Maria V. , Karyakin Vladimir B. , Zavyalov Vladimir
Published: November 2019

Epilepsy is a common neurological disorder. Despite the availability of a wide range of antiepileptic drugs, these are unsuccessful in preventing seizures in 20–30% of patients. Therefore, new pharmacological strategies are urgently required to control seizures. Modulation of glutamate uptake may have potential in the treatment of pharmacoresistant forms of epilepsy. Previous research showed that the antibiotic ceftriaxone (CTX) increased the expression and functional activity of excitatory amino acid transporter 2 (EAAT2) and exerted considerable anticonvulsant effects. However, other studies did not confirm a significant anticonvulsant effect of CTX administration. We investigated the impacts of CTX treatment on EAAT expression and glutamatergic neurotransmission, as well its anticonvulsant action, in young male Wistar rats. As shown by a quantitative real-time polymerase chain reaction (qPCR) assay and a Western blot analysis, the mRNA but not the protein level of EAAT2 increased in the hippocampus following CTX treatment. Repetitive CTX administration had only a mild anticonvulsant effect on pentylenetetrazol (PTZ)-induced convulsions in a maximal electroshock threshold test (MEST). CTX treatment did not affect the glutamatergic neurotransmission, including synaptic efficacy, short-term facilitation, or the summation of excitatory postsynaptic potentials (EPSPs) in the hippocampus and temporal cortex. However, it decreased the field EPSP (fEPSP) amplitudes evoked by intense electrical stimulation. In conclusion, in young rats, CTX treatment did not induce overexpression of EAAT2, therefore exerting only a weak antiseizure effect. Our data provide new insight into the effects of modulation of EAAT2 expression on brain functioning.
Correlations of Ion Composition and Power Efficiency in a Reverse Electrodialysis Heat Engine
Authors: Luo Fabao , Wang Yang , Sha Maolin , Wei Yanxin
Published: November 2019

The main objective of this study is to explore the influence of ion composition on the trans-membrane potential across the ion exchange membrane (IEM), and thus offers a reference for the deep insight of “reverse electrodialysis heat engine” running in the composite systems. In comparison to the natural system (river water | seawater), the performance of the reverse electrodialysis (RED) stack was examined using NaHCO3, Na2CO3, and NH4Cl as the supporting electrolyte in the corresponding compartment. The effect of flow rates and the concentration ratio in the high salt concentration compartment (HCC)/low salt concentration compartment (LCC) on energy generation was investigated in terms of the open-circuit voltage (OCV) and power density per membrane area. It was found that the new system (0.49 M NaCl + 0.01 M NaHCO3|0.01 M NaHCO3) output a relatively stable power density (0.174 W·m−2), with the open-circuit voltage 2.95 V under the low flow rate of 0.22 cm/s. Meanwhile, the simulated natural system (0.5 M NaCl|0.01 M NaCl) output the power density 0.168 W·m−2, with the open-circuit voltage 2.86 V under the low flow rate of 0.22 cm/s. The findings in this work further confirm the excellent potential of RED for the recovery of salinity gradient energy (SGP) that is reserved in artificially-induced systems (wastewaters).
New Viral Facets in Oral Diseases: The EBV Paradox
Authors: Tonoyan Lilit , Vincent-Bugnas Séverine , Olivieri Charles-Vivien , Doglio Alain
Published: November 2019

The oral cavity contributes to overall health, psychosocial well-being and quality of human life. Oral inflammatory diseases represent a major global health problem with significant social and economic impact. The development of effective therapies, therefore, requires deeper insights into the etiopathogenesis of oral diseases. Epstein–Barr virus (EBV) infection results in a life-long persistence of the virus in the host and has been associated with numerous oral inflammatory diseases including oral lichen planus (OLP), periodontal disease and Sjogren’s syndrome (SS). There is considerable evidence that the EBV infection is a strong risk factor for the development and progression of these conditions, but is EBV a true pathogen? This long-standing EBV paradox yet needs to be solved. This review discusses novel viral aspects of the etiopathogenesis of non-tumorigenic diseases in the oral cavity, in particular, the contribution of EBV in OLP, periodontitis and SS, the tropism of EBV infection, the major players involved in the etiopathogenic mechanisms and emerging contribution of EBV-pathogenic bacteria bidirectional interaction. It also proposes the involvement of EBV-infected plasma cells in the development and progression of oral inflammatory diseases. A new direction for preventing and treating these conditions may focus on controlling pathogenic EBV with anti-herpetic drugs.
microRNAs Regulating Human and Mouse Naïve Pluripotency
Authors: Wang Yuliang , Hussein Abdiasis M. , Somasundaram Logeshwaran , Sankar Rithika , Detraux Damien , Mathieu Julie , Ruohola-Baker Hannele
Published: November 2019

microRNAs are ~22bp nucleotide non-coding RNAs that play important roles in the post-transcriptional regulation of gene expression. Many studies have established that microRNAs are important for cell fate choices, including the naïve to primed pluripotency state transitions, and their intermediate state, the developmentally suspended diapause state in early development. However, the full extent of microRNAs associated with these stage transitions in human and mouse remain under-explored. By meta-analysis of microRNA-seq, RNA-seq, and metabolomics datasets from human and mouse, we found a set of microRNAs, and importantly, their experimentally validated target genes that show consistent changes in naïve to primed transitions (microRNA up, target genes down, or vice versa). The targets of these microRNAs regulate developmental pathways (e.g., the Hedgehog-pathway), primary cilium, and remodeling of metabolic processes (oxidative phosphorylation, fatty acid metabolism, and amino acid transport) during the transition. Importantly, we identified 115 microRNAs that significantly change in the same direction in naïve to primed transitions in both human and mouse, many of which are novel candidate regulators of pluripotency. Furthermore, we identified 38 microRNAs and 274 target genes that may be involved in diapause, where embryonic development is temporarily suspended prior to implantation to uterus. The upregulated target genes suggest that microRNAs activate stress response in the diapause stage. In conclusion, we provide a comprehensive resource of microRNAs and their target genes involved in naïve to primed transition and in the paused intermediate, the embryonic diapause stage.
Mycobacterium abscessus, an Emerging and Worrisome Pathogen among Cystic Fibrosis Patients
Authors: Degiacomi Giulia , Sammartino José Camilla , Chiarelli Laurent Roberto , Riabova Olga , Makarov Vadim , Pasca Maria Rosalia
Published: November 2019

Nontuberculous mycobacteria (NTM) have recently emerged as important pathogens among cystic fibrosis (CF) patients worldwide. Mycobacterium abscessus is becoming the most worrisome NTM in this cohort of patients and recent findings clarified why this pathogen is so prone to this disease. M. abscessus drug therapy takes up to 2 years and its failure causes an accelerated lung function decline. The M. abscessus colonization of lung alveoli begins with smooth strains producing glycopeptidolipids and biofilm, whilst in the invasive infection, “rough” mutants are responsible for the production of trehalose dimycolate, and consequently, cording formation. Human-to-human M. abscessus transmission was demonstrated among geographically separated CF patients by whole-genome sequencing of clinical isolates worldwide. Using a M. abscessus infected CF zebrafish model, it was demonstrated that CFTR (cystic fibrosis transmembrane conductance regulator) dysfunction seems to have a specific role in the immune control of M. abscessus infections only. This pathogen is also intrinsically resistant to many drugs, thanks to its physiology and to the acquisition of new mechanisms of drug resistance. Few new compounds or drug formulations active against M. abscessus are present in preclinical and clinical development, but recently alternative strategies have been investigated, such as phage therapy and the use of β-lactamase inhibitors.
Towards Exploring Toxin-Antitoxin Systems in Geobacillus: A Screen for Type II Toxin-Antitoxin System Families in a Thermophilic Genus
Authors: Alkhalili Rawana , Wallenius Joel , Canbäck Björn
Published: November 2019

The toxin-antitoxin (TA) systems have been attracting attention due to their role in regulating stress responses in prokaryotes and their biotechnological potential. Much recognition has been given to type II TA system of mesophiles, while thermophiles have received merely limited attention. Here, we are presenting the putative type II TA families encoded on the genomes of four Geobacillus strains. We employed the TA finder tool to mine for TA-coding genes and manually curated the results using protein domain analysis tools. We also used the NCBI BLAST, Operon Mapper, ProOpDB, and sequence alignment tools to reveal the geobacilli TA features. We identified 28 putative TA pairs, distributed over eight TA families. Among the identified TAs, 15 represent putative novel toxins and antitoxins, belonging to the MazEF, MNT-HEPN, ParDE, RelBE, and XRE-COG2856 TA families. We also identified a potentially new TA composite, AbrB-ParE. Furthermore, we are suggesting the Geobacillus acetyltransferase TA (GacTA) family, which potentially represents one of the unique TA families with a reverse gene order. Moreover, we are proposing a hypothesis on the xre-cog2856 gene expression regulation, which seems to involve the c-di-AMP. This study aims for highlighting the significance of studying TAs in Geobacillus and facilitating future experimental research.
The Good, the Bad, and the Ethical Implications of Bridging Blockchain and Multi-Agent Systems
Authors: Calvaresi Davide , Calbimonte Jean-Paul , Dubovitskaya Alevtina , Mattioli Valerio , Piguet Jean-Gabriel , Schumacher Michael
Published: November 2019

The agent based approach is a well established methodology to model distributed intelligent systems. Multi-Agent Systems (MAS) are increasingly employed in applications dealing with safety and information critical tasks (e.g., in eHealth, financial, and energy domains). Therefore, transparency and the trustworthiness of the agents and their behaviors must be enforced. For example, employing reputation based mechanisms can promote the development of trust. Nevertheless, besides recent early stage studies, the existing methods and systems are still unable to guarantee the desired accountability and transparency adequately. In line with the recent trends, we advocate that combining blockchain technology (BCT) and MAS can achieve the distribution of the trust, removing the need for trusted third parties (TTP), potential single points of failure. This paper elaborates on the notions of trust, BCT, MAS, and their integration. Furthermore, to attain a trusted environment, this manuscript details the design and implementation of a system reconciling MAS (based on the Java Agent DEvelopment Framework (JADE)) and BTC (based on Hyperledger Fabric). In particular, the agents’ interactions, computation, tracking the reputation, and possible policies for disagreement-management are implemented via smart contracts and stored on an immutable distributed ledger. The results obtained by the presented system and similar solutions are also discussed. Finally, ethical implications (i.e., opportunities and challenges) are elaborated before concluding the paper.
Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae)
Authors: Zhang Yiqiu , Xu Letian , Li Shengchun , Zhang Jiang
Published: November 2019

RNA interference (RNAi) has emerged as a novel and feasible strategy for pest management. Methods for cost-effective production and stable delivery of double-stranded RNA (dsRNA) to the target insects are crucial for the wide application of RNAi for pest control. In this study, we tested the expression of dsRNA in RNaseIII-deficient Escherichia coli HT115 which was then fed to Plagiodera versicolora larvae, an insect pest of Salicaceae plants worldwide. By targeting six potential genes, including actin (ACT), signal recognition particle protein 54k (SRP54), heat shock protein 70 (HSC70), shibire (SHI), cactus (CACT), and soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAP), we found that feeding bacteria-expressed dsRNA successfully triggered the silencing of the five target genes tested and the suppression of ACT and SRP54 genes caused significant mortality. Our results suggest that the oral delivery of bacteria-expressed dsRNA is a potential alternative for the control of P. versicolora, and that ACT and SRP54 genes are the potent targets.
Age and Method of Inoculation Influence the Infection of Worker Honey Bees (Apis mellifera) by Nosema ceranae
Authors: Urbieta-Magro Almudena , Higes Mariano , Meana Aránzazu , Barrios Laura , Martín-Hernández Raquel
Published: November 2019

The microsporidian parasite Nosema ceranae is a highly prevalent, global honey bee pathogen. Apis mellifera is considered to be a relatively recent host for this microsporidia, which raises questions as to how it affects its host’s physiology, behavior and longevity, both at the individual and colony level. As such, honey bees were inoculated with fresh purified spores of this pathogen, both individually (Group A) or collectively (Group B) and they were studied from 0 to 15 days post-emergence (p.e.) to evaluate the effect of bee age and the method of inoculation at 7 days post-infection. The level of infection was analyzed individually by qPCR by measuring the relative amount of the N. ceranae polar tubule protein 3 (PTP3) gene. The results show that the bee’s age and the method of infection directly influence parasite load, and thus, early disease development. Significant differences were found regarding bee age at the time of infection, whereby the youngest bees (new-born and 1 day p.e.) developed the highest parasite load, with this load decreasing dramatically in bees infected at 2 days p.e. before increasing again in bees infected at 3–4 days p.e. The parasite load in bees infected when older than 4 days p.e. diminished as they aged. When the age cohort data was pooled and grouped according to the method of infection, a significantly higher mean concentration and lower variation in N. ceranae infection was evident in Group A, indicating greater variation in experimental infection when spores were administered collectively to bees through their food. In summary, these data indicate that both biological and experimental factors should be taken into consideration when comparing data published in the literature.
Regorafenib CSF Penetration, Efficacy, and MRI Patterns in Recurrent Malignant Glioma Patients
Authors: Zeiner Pia S. , Kinzig Martina , Divé Iris , Maurer Gabriele D. , Filipski Katharina , Harter Patrick N. , Senft Christian , Bähr Oliver , Hattingen Elke , Steinbach Joachim P. , Sörgel Fritz , Voss Martin , Steidl Eike , Ronellenfitsch Michael W.
Published: November 2019

(1) Background: The phase 2 Regorafenib in Relapsed Glioblastoma (REGOMA) trial indicated a survival benefit for patients with first recurrence of a glioblastoma when treated with the multikinase inhibitor regorafenib (REG) instead of lomustine. The aim of this retrospective study was to investigate REG penetration to cerebrospinal fluid (CSF), treatment efficacy, and effects on magnetic resonance imaging (MRI) in patients with recurrent high-grade gliomas. (2) Methods: Patients were characterized by histology, adverse events, steroid treatment, overall survival (OS), and MRI growth pattern. REG and its two active metabolites were quantified by liquid chromatography/tandem mass spectrometry in patients’ serum and CSF. (3) Results: 21 patients mainly with IDH-wildtype glioblastomas who had been treated with REG were retrospectively identified. Thirteen CFS samples collected from 3 patients of the cohort were available for pharmacokinetic testing. CSF levels of REG and its metabolites were significantly lower than in serum. Follow-up MRI was available in 19 patients and showed progressive disease (PD) in all but 2 patients. Two distinct MRI patterns were identified: 7 patients showed classic PD with progression of contrast enhancing lesions, whereas 11 patients showed a T2-dominant MRI pattern characterized by a marked reduction of contrast enhancement. Median OS was significantly better in patients with a T2-dominant growth pattern (10 vs. 27 weeks respectively, p = 0.003). Diffusion restrictions were observed in 13 patients. (4) Conclusion: REG and its metabolites were detectable in CSF. A distinct MRI pattern that might be associated with an improved OS was observed in half of the patient cohort. Treatment response in the total cohort was poor.
Association of Pulse Volume Recording at Ankle with Total and Cardiovascular Mortality in Hemodialysis Patients
Authors: Lee Wen-Hsien , Hsu Po-Chao , Huang Jiun-Chi , Chen Ying-Chih , Chen Szu-Chia , Wu Pei-Yu , Lee Meng-Kuang , Lee Chee-Siong , Yen Hsueh-Wei , Su Ho-Ming
Published: November 2019

Pulse volume recording is an accurate modality for detecting arterial occlusion in the lower extremities. There are two indexes of pulse volume recording measured at ankle, percentage of mean arterial pressure (%MAP) and upstroke time (UT). The aim of the study was to examine the ability of %MAP and UT for the prediction of overall and cardiovascular mortality in hemodialysis (HD) patients. In 197 routine HD patients, ankle %MAP, ankle UT, and ankle–brachial index (ABI) were automatically measured by Colin VP-1000 instrument. Fourteen cardiovascular mortality and 29 overall mortalities were documented during 2.7 ± 0.6 years follow-up. In the univariate analysis, in addition to co-morbidities and traditional clinical parameters, increased total mortality was associated with decreased ABI, ABI < 0.9, increased %MAP and UT, %MAP > 50%, and UT > 169 ms (p ≤ 0.041) and increased cardiovascular mortality was associated with increased UT and %MAP > 50% (p ≤ 0.022). After multivariate analysis, increased %MAP and %MAP > 50% (p ≤ 0.047) were still the predictors of total mortality and %MAP > 50% (p = 0.024) was still the predictor of cardiovascular mortality. In HD patients, we found that ankle %MAP and %MAP > 50% could predict total mortality and ankle %MAP > 50% could predict cardiovascular mortality in the multivariate analysis. Hence, assessment of %MAP from pulse volume recording at ankle might be helpful in identifying the high-risk group for poor prognosis in HD patients.
Hospital Malnutrition, a Call for Political Action: A Public Health and NutritionDay Perspective
Authors: Hiesmayr Michael , Tarantino Silvia , Moick Sigrid , Laviano Alessandro , Sulz Isabella , Mouhieddine Mohamed , Schuh Christian , Volkert Dorothee , Simon Judit , Schindler Karin
Published: November 2019

Disease-related malnutrition (DRM) is prevalent in hospitals and is associated with increased care needs, prolonged hospital stay, delayed rehabilitation and death. Nutrition care process related activities such as screening, assessment and treatment has been advocated by scientific societies and patient organizations but implementation is variable. We analysed the cross-sectional nutritionDay database for prevalence of nutrition risk factors, care processes and outcome for medical, surgical, long-term care and other patients (n = 153,470). In 59,126 medical patients included between 2006 and 2015 the prevalence of recent weight loss (45%), history of decreased eating (48%) and low actual eating (53%) was more prevalent than low BMI (8%). Each of these risk factors was associated with a large increase in 30 days hospital mortality. A similar pattern is found in all four patient groups. Nutrition care processes increase slightly with the presence of risk factors but are never done in more than 50% of the patients. Only a third of patients not eating in hospital receive oral nutritional supplements or artificial nutrition. We suggest that political action should be taken to raise awareness and formal education on all aspects related to DRM for all stakeholders, to create and support responsibilities within hospitals, and to create adequate reimbursement schemes. Collection of routine and benchmarking data is crucial to tackle DRM.
The Role of Animal Models in Developing Pharmacotherapy for Obstructive Sleep Apnea
Authors: Kim Lenise Jihe , Freire Carla , Fleury Curado Thomaz , Jun Jonathan C. , Polotsky Vsevolod  Y.
Published: November 2019

Obstructive sleep apnea (OSA) is a highly prevalent disease characterized by recurrent closure of the upper airway during sleep. It has a complex pathophysiology involving four main phenotypes. An abnormal upper airway anatomy is the key factor that predisposes to sleep-related collapse of the pharynx, but it may not be sufficient for OSA development. Non-anatomical traits, including (1) a compromised neuromuscular response of the upper airway to obstruction, (2) an unstable respiratory control (high loop gain), and (3) a low arousal threshold, predict the development of OSA in association with anatomical abnormalities. Current therapies for OSA, such as continuous positive airway pressure (CPAP) and oral appliances, have poor adherence or variable efficacy among patients. The search for novel therapeutic approaches for OSA, including pharmacological agents, has been pursued over the past years. New insights into OSA pharmacotherapy have been provided by preclinical studies, which highlight the importance of appropriate use of animal models of OSA, their applicability, and limitations. In the present review, we discuss potential pharmacological targets for OSA discovered using animal models.
Simulated Performance of a Xenohybrid Bone Graft (SmartBone®) in the Treatment of Acetabular Prosthetic Reconstruction
Authors: Grottoli Carlo Francesco , Cingolani Alberto , Zambon Fabio , Ferracini Riccardo , Villa Tomaso , Perale Giuseppe
Published: November 2019

Total hip arthroplasty (THA) is a surgical procedure for the replacement of hip joints with artificial prostheses. Several approaches are currently employed in the treatment of this kind of defect. Overall, the most common method involves using a quite invasive metallic support (a Burch–Schneider ring). Moreover, valid alternatives and less invasive techniques still need to be supported by novel material development. In this work, we evaluated the performance of SmartBone®, a xenohybrid bone graft composed of a bovine bone matrix reinforced with biodegradable polymers and collagen, as an effective support in acetabular prosthesis reconstruction. Specifically, the material’s mechanical properties were experimentally determined (E = ~1.25 GPa, Ef = ~0.34 GPa, and Et = ~0.49 GPa) and used for simulation of the hip joint system with a SmartBone® insert. Moreover, a comparison with a similar case treated with a Burch–Schneider ring was also conducted. It was found that it is possible to perform THA revision surgeries without the insertion of an invasive metal support and it can be nicely combined with SmartBone®’s osteointegration characteristics. The material can withstand the loads independently (σmax = ~12 MPa) or be supported by a thinner titanium plate in contact with the bone in the worst cases. This way, improved bone regeneration can be achieved.
Morphing Hydrofoil Model Driven by Compliant Composite Structure and Internal Pressure
Authors: Fatiha Mohammed Arab , Augier Benoît , Deniset François , Casari Pascal , Astolfi Jacques André
Published: November 2019

In this work, a collaborative experimental study has been conducted to assess the effect an imposed internal pressure has on the controlling the hydrodynamic performance of a compliant composite hydrofoil. It was expected that the internal pressure together with composite structures be suitable to control the hydrodynamic forces as well as cavitation inception and development. A new concept of morphing hydrofoil was developed and tested in the cavitation tunnel at the French Naval Academy Research Institute. The experiments were based on the measurements of hydrodynamic forces and hydrofoil deformations under various conditions of internal pressure. The effect on cavitation inception was studied too. In parallel to this experiment, a 2D numerical tool was developed in order to assist the design of the compliant hydrofoil shape. Numerically, the fluid-structure coupling is based on an iterative method under a small perturbation hypothesis. The flow model is based on a panel method and a boundary layer formulation and was coupled with a finite-element method for the structure. It is shown that pressure driven compliant composite structure is suitable to some extent to control the hydrodynamic forces, allowing the operational domain of the compliant hydrofoil to be extended according to the angle of attack and the internal pressure. In addition, the effect on the cavitation inception is pointed out.
Development of a Model Test System for a Piston Ring/Cylinder Liner-Contact with Focus on Near-to-Application Seizure Behaviour
Authors: Pusterhofer Michael , Summer Florian , Wuketich Daniel , Grün Florian
Published: November 2019

Physical simulations of tribo contacts in internal combustion engines can act as a supporting tool to match upcoming guidelines and emission restrictions. In particular, the scuffing resistance of the contact between the piston ring and cylinder liner suffers under decreasing oil viscosity and limitation of antiwear additives. This paper aims to provide an experimental method to simulate the scuffing of the piston ring/cylinder liner-contact and to validate this method with real engine parts and the literature from engine tests. The experimental methodology uses a linear tribometer TE77 to test specimens from original piston rings and liners under reciprocating motion. Additionally, the ring specimen is given the opportunity to perform secondary movements (ring twisting, ring turning) and to run under deficient lubrication conditions similar to the engine. A specially designed test strategy enables the reproducible creation of seizure of the tribosystem. The seizure resistance of two engine oils, tested for validation, correlates with the known engine performance. Therefore, the model test system can be seen as a reproducible tool for simulating seizure of a ring/liner-system, showing similar trends and wear mechanisms as in an engine. Surface analysis depicts similarities between the scuffed surfaces of an engine and the model and discusses the origin of seizure based on the model specimens together with the relevant literature.
Paenidigyamycin G: 1-Acetyl-2,4-dimethyl-3-phenethyl-1H-imidazol-3-ium
Authors: Tetevi Gilbert Mawuli , Kwain Samuel , Mensah Thomas , Camas Anil Sazak , Camas Mustafa , Dofuor Aboagye Kwarteng , Azerigyik Faustus Akankperiwen , Oluwabusola Emmanuel , Deng Hai , Jaspars Marcel , Kyeremeh Kwaku
Published: November 2019

The Ghanaian Paenibacillus sp. DE2SH (GenBank Accession Number: MH091697) is a prolific producer of potent antiparasitic alkaloids. Further detailed study of the culture broth of this strain produced the compound Paenidigyamycin G (1), which is a derivative of the known antiparasitic compound Paenidigyamycin A (2). Compound (1) was isolated on HPLC at tR ≈ 37.5 min and its structure determined by IR, UV, MS, 1D, and 2D-NMR data. Compound 1 produced weak to moderate antileishmanial and antitrypanosomal activity when tested against Leishmania donovani (Laveran and Mesnil) Ross (D10) and Trypanosoma brucei subsp. brucei strain GUTat 3.1 with IC50 = 115.41 and 28.75 μM, respectively. This result is interesting since the parent compound 2 is known to possess consistent and potent antiparasitic activity. However, 1 displayed a promising selectivity profile towards T. brucei subsp. brucei due to its relatively low toxicity against normal mouse macrophages RAW 264.7 cells (SI = 8.70). Given that compound 1 is also the main metabolite found in the hexane fraction of all extracts produced by Paenibacillus sp. DE2SH when it is co-cultured with other bacteria strains, it must possess some unique biological functions which should make it an excellent candidate for further biological activity screening in other bioassays.
Magnesium Phosphate Cement as Mineral Bone Adhesive
Authors: Brückner Theresa , Meininger Markus , Groll Jürgen , Kübler Alexander C. , Gbureck Uwe
Published: November 2019

Mineral bone cements were actually not developed for their application as bone-bonding agents, but as bone void fillers. In particular, calcium phosphate cements (CPC) are considered to be unsuitable for that application, particularly under moist conditions. Here, we showed the ex vivo ability of different magnesium phosphate cements (MPC) to adhere on bovine cortical bone substrates. The cements were obtained from a mixture of farringtonite (Mg3(PO4)2) with different amounts of phytic acid (C6H18O24P6, inositol hexaphosphate, IP6), whereas cement setting occurred by a chelation reaction between Mg2+ ions and IP6. We were able to show that cements with 25% IP6 and a powder-to-liquid ratio (PLR) of 2.0 g/mL resulted in shear strengths of 0.81 ± 0.12 MPa on bone even after 7 d storage in aqueous conditions. The samples showed a mixed adhesive–cohesive failure with cement residues on the bone surface as indicated by scanning electron microscopy and energy-dispersive X-ray analysis. The presented material demonstrated appropriate bonding characteristics, which could enable a broadening of the mineral bone cements’ application field to bone adhesives.
Finite Element Method-Based Skid Resistance Simulation Using In-Situ 3D Pavement Surface Texture and Friction Data
Authors: Peng Yi , Li Joshua Qiang , Zhan You , Wang Kelvin C. P. , Yang Guangwei
Published: November 2019

Skid resistance is an important surface characteristic that influences roadway safety. Various studies have been performed to understand the interaction between pavement and tires through numerical simulation for skid resistance prediction. However, the friction parameters required for simulation inputs are generally determined by objective assumptions. This paper develops a finite element method (FEM)-based skid resistance simulation framework using in-situ 3D pavement surface texture and skid resistance data. A 3D areal pavement model is reconstructed from high resolution asphalt pavement surface texture data. The exponential decay friction model is implemented in the simulation and the interface friction parameters required for the simulation are determined using the binary search back-calculation approach based on a trial process with the desired level of differences between simulated and observed skid numbers. To understand the influence of texture characteristics on interface friction parameters, the high-resolution 3D texture data is separated into macro- and micro-scales through Butterworth filtering and various areal texture indicators are calculated at both levels. Principal component analysis (PCA) regression analysis is conducted to quantify the relationship between various texture characteristics and the interface friction parameters. The results from this study can be used to better prepare the inputs of friction parameters for FEM simulation.
Heat Treatment, Impact Properties, and Fracture Behaviour of Ti-6Al-4V Alloy Produced by Powder Compact Extrusion
Authors: Singh Ajit Pal , Yang Fei , Torrens Rob , Gabbitas Brian
Published: November 2019

The mechanical properties of titanium and titanium alloys are very sensitive to processing, microstructure, and impurity levels. In this paper, a blended powder mixture of Ti-6Al-4V alloy was consolidated by powder compact extrusion that involved warm compaction, vacuum sintering, and hot extrusion. The as-processed material with an oxygen content of 0.34 wt.% was subjected to various annealing treatments. The impact toughness of heat-treated material was determined using Charpy V-notch impact testing at room temperature. An emphasis was placed on establishing a relationship among fracture behaviour, microstructure, and the resulting properties of tested material. From the results, it is apparent that the highest impact toughness value of 19.3 J was achieved after α/β annealing and is comparable with typical values given in the literature for wrought Ti-6Al-4V. In terms of fracture behaviour, it is quite apparent that the crack propagation behaviour of powder-produced material is rather complex compared with the limited amount of data reported for ingot counterparts.
Microwave-Assisted Synthesis and Characterization of Poly(L-lysine)-Based Polymer/Carbon Quantum Dot Nanomaterials for Biomedical Purposes
Authors: Janus Łukasz , Piątkowski Marek , Radwan-Pragłowska Julia
Published: November 2019

Carbon nanomaterials in the form of quantum dots have a high potential due to their luminescent properties and low cytotoxicity which allows their use in optical probes for use in bioimaging and biodetection. In this article, we present a novel type of nanomaterials and their obtainment method under microwave-assisted conditions using poly(L-lysine) as a raw material. The ready products were characterized over their chemical structure, pH-dependent fluorescence properties and cytotoxicity on human dermal fibroblasts. Moreover, their antioxidant activity as well as ability to biologically active molecules (vitamins) and heavy metal ions detection was evaluated. The results confirmed the obtainment of biocompatible nanomaterials with advanced properties and good water solubility according to sustained development principles.
Can Sub-zero Treatment at −75 °C Bring Any Benefits to Tools Manufacturing?
Authors: Kusý Martin , Rízeková-Trnková Lýdia , Krajčovič Jozef , Dlouhý Ivo , Jurči Peter
Published: November 2019

: Vanadis 6 ledeburitic tool steel was subjected to sub-zero treatment at −75 °C for different durations, and for different subsequent tempering regimes. The impact of these treatments on the microstructure, hardness variations, and toughness characteristics of the steel was investigated. The obtained results infer that the retained austenite amount was reduced to one fourth by sub-zero treatment (SZT), and the population density of add-on carbides was increased by factor of three to seven, depending on the duration of SZT. Tempering always reduced the population density of these particles. A hardness increased by 30–60 HV10 was recorded after sub-zero treatment but tempering to the secondary hardness peak induced much more significant hardness decrease than what was established in conventionally quenched steel. The flexural strength was not negatively influenced by sub-zero treatment at −75 °C while the fracture toughness tests gave worse values of this quantity, except the case of steel tempered to the secondary hardness peak.