bluuebunny/crossref_metadata_2025 huggingface dataset:
The lncRNA H19 binding to let‐7b promotes hippocampal glial cell activation and epileptic seizures by targeting Stat3 in a rat model of temporal lobe epilepsy
Authors: Han Chun‐Lei , Liu Yun‐Peng , Guo Chen‐Jia , Du Ting‐Ting , Jiang Ying , Wang Kai‐Liang , Shao Xiao‐Qiu , Meng Fan‐Gang , Zhang Jian‐Guo
Published: July 2020

Objectives

Glial cell activation contributes to the inflammatory response and occurrence of epilepsy. Our preliminary study demonstrated that the long non‐coding RNA, H19, promotes hippocampal glial cell activation during epileptogenesis. However, the precise mechanisms underlying this effect remain unclear.

Materials and methods

H19 and let‐7b were overexpressed or silenced using an adeno‐associated viral vector in vivo. Their expression in a kainic acid‐induced epilepsy model was evaluated by real‐time quantitative PCR, fluorescence in situ hybridization, and cytoplasmic and nuclear RNA isolation. A dual‐luciferase reporter assay was used to evaluate the direct binding of let‐7b to its target genes and H19. Western blot, video camera monitoring and Morris water maze were performed to confirm the role of H19 and let7b on epileptogenesis.

Results

H19 was increased in rat hippocampus neurons after status epilepticus, which might be due to epileptic seizure‐induced hypoxia. Increased H19 aggravated the epileptic seizures, memory impairment and mossy fibre sprouting of the epileptic rats. H19 could competitively bind to let‐7b to suppress its expression. Overexpression of let‐7b inhibited hippocampal glial cell activation, inflammatory response and epileptic seizures by targeting Stat3. Moreover, overexpressed H19 reversed the inhibitory effect of let‐7b on glial cell activation.

Conclusions

LncRNA H19 could competitively bind to let‐7b to promote hippocampal glial cell activation and epileptic seizures by targeting Stat3 in a rat model of temporal lobe epilepsy.
Caring babies: Concern for others in distress during infancy
Authors: Davidov Maayan , Paz Yael , Roth‐Hanania Ronit , Uzefovsky Florina , Orlitsky Tal , Mankuta David , Zahn‐Waxler Carolyn
Published: July 2020

Concern for distressed others is a highly valued human capacity, but little is known about its early ontogeny. Theoretical accounts of empathy development have emphasized stages, but this has been called into question. This study sheds new light on four key issues: onset, consistency, development, and predictive power of early manifestations of concern for others. Three‐month‐old Israei infants (N = 165) were followed longitudinally at ages 6, 12, and 18 months, and their observed responses to others’ distress were assessed. Concern for distressed others was seen early in the first year of life, long before previous theories assumed. Empathic concern was moderately consistent across both situation and age, from as early as 3 months. Concern for others grew only modestly with age, plateauing during the second year, whereas prosocial behavior increased rapidly during the second year. Early individual differences in concern for others predicted later prosocial behavior on behalf of distressed others. Findings underscore the early roots of caring, and appear to refute assumptions of prior stage theories of empathy development, by showing that concern for others develops much earlier and more gradually than previously assumed.
Development and validation of a novel 15‐CpG‐based signature for predicting prognosis in triple‐negative breast cancer
Authors: Peng Yang , Shui Lin , Xie Jian , Liu Shengchun
Published: July 2020

DNA methylation is an important biological regulatory mechanism that changes gene expression without altering the DNA sequence. Increasing studies have revealed that DNA methylation data play a vital role in the field of oncology. However, the methylation site signature in triple‐negative breast cancer (TNBC) remains unknown. In our research, we analysed 158 TNBC samples and 98 noncancerous samples from The Cancer Genome Atlas (TCGA) in three phases. In the discovery phase, 86 CpGs were identified by univariate Cox proportional hazards regression (CPHR) analyses to be significantly correlated with overall survival (P < 0.01). In the training phase, these candidate CpGs were further narrowed down to a 15‐CpG‐based signature by conducting least absolute shrinkage and selector operator (LASSO) Cox regression in the training set. In the validation phase, the 15‐CpG‐based signature was verified using two different internal sets and one external validation set. Furthermore, a nomogram comprising the CpG‐based signature and TNM stage was generated to predict the 1‐, 3‐ and 5‐year overall survival in the primary set, and it showed excellent performance in the three validation sets (concordance indexes: 0.924, 0.974 and 0.637). This study showed that our nomogram has a precise predictive effect on the prognosis of TNBC and can potentially be implemented for clinical treatment and diagnosis.
Infestation of fruit by conspecific and heterospecific females deters oviposition in two Tephritidae fruit fly species
Authors: Liendo María Clara , Parreño María Alejandra , Pietrek Alejandro G. , Bouvet Juan Pedro , Milla Fabián H. , Vera M. Teresa , Cladera Jorge L. , Segura Diego F.
Published: July 2020

Tephritidae fruit fly larvae develop entirely in the host chosen by the females. To improve the fitness of their progeny, females would benefit from rejecting previously exploited hosts. Anastrepha fraterculus and Ceratitis capitata are two species of fruit flies having similar nutritional requirements and overlapping in their distribution. Previous studies found that competition between the larvae of these species might reach high levels, suggesting that cross‐recognition would be an adaptive trait. In this work, we tested the ability of A. fraterculus and C. capitata females to recognize and avoid fruits previously infested by both conspecific and heterospecific females. In laboratory behavioural arenas, females were presented with fruits that had been previously exposed to either conspecific or heterospecific females. Then, we conducted choice and non‐choice assays to compare the response of A. fraterculus and C. capitata females to infested and non‐infested fruits. In non‐choice tests, the females from both species rejected fruits previously infested by conspecific and heterospecific individuals. However, the rejection occurred at different steps of the sequence leading to oviposition: A. fraterculus showed a lower rate of visits to infested fruits, whereas C. capitata visited both infested and non‐infested fruits, but the latency to visit a fruit and the rejection frequency were higher and the duration of the visit to infested fruit was lower. In choice assays, the rejection of heterospecific infested fruit was higher than that of conspecific infested fruits, for both species. Our results suggest that, regardless of the sensory mechanism used by females, the recognition of previous infestation is bidirectional and females of both species, belonging to different genera, recognize fruit infested by heterospecifics. These responses indicate that cross‐recognition, supposedly a highly beneficial trait, could be occurring in nature, thus reducing interspecific competition and contributing to the coexistence of these species.
The “ART” of facial filler injections: Avoid, recognize, and treat hyaluronic acid‐induced complications
Authors: Zeltzer Assaf , Geeroms Maxim , Antoniazzi Elisa , Giunta Gabriele , De Baerdemaeker Randy , Hendrickx Benoit , Hamdi Moustapha
Published: July 2020

Background

Hyaluronic acid (HA) fillers are frequently used for cosmetic purposes as volumizers or as wrinkle fillers. One of the major advantages of hyaluronic acid use is the possibility to neutralize it by using hyaluronidase should complications occur.

Patient

A case of a 21‐year‐old female patient is presented, in whom a hyaluronic acid injection‐induced vascular occlusion was seen four days after the initial injection, with increasing pain and severe signs of ischemia.

Method

Treatment consisted of an immediate hyaluronidase injection, supplemented with administration of acetylsalicylic acid, piracetam, low molecular weight heparin (LMWH), corticosteroids, analgesics, prophylactic antibiotics, application of topical nitroglycerin and warmth, smoking cessation, and hyperbaric oxygen therapy.

Results

The initial progress and evolution of the deformity with possible therapeutic options are being discussed.

Conclusion

The “ART” (avoid, recognize, and treat) in handling filler complications is presented, as a new universal guideline for clinical aesthetic practitioners and injectors.
Singularity in the wake of slavery: Adriana Cavarero's ontology of uniqueness and Alex Haley's Roots
Authors: Söderbäck Fanny
Published: July 2020

This essay examines Italian philosopher Adriana Cavarero's ontology of uniqueness through a reading of Alex Haley's novel Roots, and the recent television adaptation of that book. If Cavarero has insisted throughout her work that we need to challenge the philosophical privileging of abstract universality and focus instead on the irreducibility of embodied singularity, and if such a move in her work has always relied on a feminist analysis of the role women play in such a drama, I argue that attention to issues of race and the institution of slavery both supports and complicates her analysis in meaningful ways.
Peter Winch: “Unity: Presupposition or Demand?”
Authors: Burns Steven
Published: July 2020

This is a translation of a paper which the late Peter Winch wrote in German for a 1987 conference. He deals with fundamental issues in ethics, especially with the Wittgensteinian idea that “primitive reactions” play a crucial role in the formation of moral concepts. It also responds to an important objection, namely that primitive reactions can be as much immoral as moral. Ranging as it does over Winch’s interests in Wittgenstein, Simone Weil and Plato, the paper can serve as a concise introduction to Winch’s work.
TEACH and repeat: Deliberate practice for teaching
Authors: Marcus Carolyn H , Newman Lori R , Winn Ariel S , Antanovich Kate , Audi Zeena , Cohen Amy , Hirsch Alexander W , Harris Holly K , Miller Kelsey A , Michelson Catherine D
Published: July 2020

Background

Deliberate practice facilitates skill mastery. We aimed to create a novel resident‐as‐teacher rotation, leveraging a deliberate practice framework with repeated practice in real‐life teaching settings, with feedback from dedicated faculty member coaches.

Methods

A resident‐as‐teacher rotation was designed for 35 Postgraduate Year‐2 (PGY2) paediatric residents. To facilitate deliberate practice, teaching experiences were observed by faculty member coaches and were repeated with different audiences. Participating residents received pre‐rotation, post‐rotation and follow‐up surveys on their confidence and comfort with teaching, supervision and feedback skills. All residents were also surveyed the year before and the year after implementation on their perceptions of their teaching, supervision and feedback skills, and whether the residency provided adequate training on these topics.

Results

Survey response rates varied from 40 to 71%. The rotation was highly valued, and deliberate practice was a most valued aspect. Mean scores in comfort and confidence significantly increased from pre‐ to post‐rotation, with these increases sustained months later. Prior to implementation, residents’ perceptions of their teaching skills and the adequacy of their training increased incrementally with each postgraduate year. After the inaugural year of the rotation, the PGY2 class rated their teaching skills and training as higher than more experienced residents.

Discussion

A novel resident‐as‐teacher rotation successfully incorporated deliberate practice in real‐life settings by repeating teaching activities with feedback from dedicated coaches. The rotation led to sustained increases in residents’ confidence in their teaching, supervising and feedback skills, and improved perceptions of their teaching training during residency.
Geochemical study of Cenozoic mafic volcanism in the west-central Great Basin, western Nevada, and the Ancestral Cascades Arc, California
Authors: Timmermans Ann C. , Cousens Brian L. , Henry Christopher D.
Published: July 2020

Processes linked to shallow subduction, slab rollback, and extension are recorded in the whole-rock major-, trace-element, and Sr, Nd, and Pb isotopic compositions of mafic magmatic rocks in both time and space over southwestern United States. Eocene to Mio-Pliocene volcanic rocks were sampled along a transect across the west-central Great Basin (GB) in Nevada to the Ancestral Cascade Arc (ACA) in the northern Sierra Nevada, California (∼39°–40° latitude), which are interpreted to represent a critical segment of a magmatic sweep that occurred as a result of subduction from east-northeast convergence between the Farallon and North American plates and extension related to the change from a convergent to a transform margin along the western edge of North America.

Mafic volcanic rocks from the study area can be spatially divided into three broad regions: GB (5–35 Ma), eastern ACA, and western ACA (2.5–16 Ma). The volcanic products are dominantly calc-alkalic but transition to alkalic toward the east. Great Basin lavas erupted far inland from the continental margin and have higher K, P, Ti, and La/Sm as well as lower (Sr/P)pmn, Th/Rb, and Ba/Nb compared to ACA lavas. Higher Pb isotopic values, combined with lower Ce/Ce* and high Th/Nb ratios in some ACA lavas, are interpreted to come from slab sediment. Mafic lavas from the GB and ACA have overlapping 87Sr/86Sr and 143Nd/144Nd values that are consistent with mantle wedge melts mixing with a subduction-modified lithospheric mantle source. Eastern and western ACA lavas largely overlap in age and elemental and isotopic composition, with the exception of a small subset of lavas from the westernmost ACA region; these lavas show lower 87Sr/86Sr at a given 143Nd/144Nd. Results show that although extension contributes to melting in some regions (e.g., selected lavas in the GB and Pyramid Lake), chemical signatures for most mafic melts are dominated by subduction-related mantle wedge and a lithospheric mantle component.
Compact Circular CSRR Loaded Koch Fractal Inspired Frequency Band Reconfigurable Antenna
Authors: Saha Puspendu Bikash , Ghoshal Dibyendu , Dash Rajib Kumar
Published: July 2020

A compact frequency band reconfigurable dual-mode antenna utilizing both Koch fractal geometry and circular complementary split ring resonators (CSRRs) is presented in this paper. The proposed antenna can be electrically switched into both dual- and single-band resonance modes. A Koch curve derivative novel fractal curve is used to design the patch boundary of the antenna. Two optimized circular CSRRs are loaded inside the antenna geometry to achieve double-band resonance. The antenna operates at both 5.1[Formula: see text]GHz (upper WLAN band) and 9.6[Formula: see text]GHz ([Formula: see text]-band) in dual resonance mode and resonates only at 8[Formula: see text]GHz ([Formula: see text]-band) in single resonance mode. A properly biased RF-range PIN diode placed at the slotted ground plane reconfigures the antenna in two operating modes by switching into its ON–OFF states. The antenna is fabricated on cost-efficient epoxy (FR-4) substrate consuming the maximum dimension of [Formula: see text] which is much more compact than the conventional rectangular patch antennas designed for similar bands. Stable radiation characteristics with reduced cross-polarization are achieved at all the resonant bands of the antenna. A prototype of the proposed antenna is fabricated and measured. The simulated results are in good agreement with the measured ones.
Reduced Duration of Postchikungunya Musculoskeletal Pain in Rheumatological Patients Treated with Biologicals
Authors: Brito Carlos A. A. de , Marques Claudia D. L. , França Rafael F. O. , Monteiro Janaína R. , de Brito Marina C. M. , Lopes Fabiana , Lima Anton , Marques Gabriel L. , Valadares Maria Luisa , Duarte Angela L. B. P.
Published: July 2020

Chikungunya fever (CHIK) has caused important epidemic outbreaks in the Americas, with musculoskeletal involvement being the main manifestation, causing chronic symptoms in half of the affected patients. This study was performed to evaluate the clinical course of the infection in 168 patients with autoimmune inflammatory disease using biological disease-modifying antirheumatic drugs (bDMARDs), comparing this group with 56 household controls. Anti-CHIKV IgG serology was positive in 42 (25%) of the patients in the bDMARD group and in 15 (27%) of the controls (p=0.79). Of those with positive serology, 32 (76%) and 14 (93%) were symptomatic among subjects in the bDMARD and control groups, respectively (p=0.87). Persistence of musculoskeletal symptoms for more than three months occurred in 64% of the patients in the control group and only in 28% in the bDMARD group (p=0.021), maintaining a statistically significant difference only for users of anti-TNF. This study found that patients affected by chikungunya fever using bDMARDs did not present severe forms or complications in the acute phase of the disease, and patients using anti-TNF biologicals had a lower frequency of chronic joint symptoms than the household controls. This favorable outcome may be related to the cytokine blockade, with a reduction in the inflammatory response and joint damage.
Computationally Efficient Ambiguity-Free Two-Dimensional DOA Estimation Method for Coprime Planar Array: RD-Root-MUSIC Algorithm
Authors: Chen Luo , Ye Changbo , Li Baobao
Published: July 2020

While the two-dimensional (2D) spectral peak search suffers from expensive computational burden in direction of arrival (DOA) estimation, we propose a reduced-dimensional root-MUSIC (RD-Root-MUSIC) algorithm for 2D DOA estimation with coprime planar array (CPA), which is computationally efficient and ambiguity-free. Different from the conventional 2D DOA estimation algorithms based on subarray decomposition, we exploit the received data of the two subarrays jointly by mapping CPA to the full array of the CPA (FCPA), which contributes to the enhanced degrees of freedom (DOFs) and improved estimation performance. In addition, due to the ambiguity-free characteristic of the FCPA, the extra ambiguity elimination operation can be avoided. Furthermore, we convert the 2D spectral search process into 1D polynomial rooting via reduced-dimension transformation, which substantially reduces the computational complexity while preserving the estimation accuracy. Finally, numerical simulations demonstrate the superiority of the proposed algorithm.
Simultaneous Voltammetric Determination of Uric Acid, Xanthine, and Hypoxanthine Using CoFe2O4/Reduced Graphene Oxide-Modified Electrode
Authors: Hoan Nguyen Thi Vuong , Minh Nguyen Ngoc , Trang Nguyen Thi Hong , Thuy Le Thi Thanh , Van Hoang Cao , Mau Tran Xuan , Vu Ho Xuan Anh , Thu Phan Thi Kim , Phong Nguyen Hai , Khieu Dinh Quang
Published: July 2020

In the present paper, the synthesis of cobalt ferrite/reduced graphene oxide (Co2Fe2O4/rGO) composite and its use for the simultaneous determination of uric acid (UA), xanthine (XA), and hypoxanthine (HX) is demonstrated. Cobalt ferrite hollow spheres were synthesized by using the carbonaceous polysaccharide microspheres prepared from aD-glucose solution as templates, followed by calcination. The CoFe2O4/rGO composite was prepared with the ultrasound-assisted method. The obtained material was characterized by using X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, EDX elemental mapping, and nitrogen adsorption/desorption isotherms. The electrochemical behavior of UA, XA, and HX on the CoFe2O4/rGO-modified electrode was studied with cyclic voltammetry and differential pulse voltammetry (DPV). The modified electrode exhibits excellent electrocatalytic activity towards the oxidation of the three compounds. The calibration curves for UA, XA, and HX were obtained over the range of 2.0–10.0 μM from DPV. The limits of detection for UA, XA, and HX are 0.767, 0.650, and 0.506 μM, respectively. The modified electrode was applied to the simultaneous detection of UA, XA, and HX in human urine, and the results are consistent with those obtained from the high-performance liquid chromatography technique.
Genomic Profiling of Prostate Cancers from Men with African and European Ancestry
Authors: Koga Yusuke , Song Hanbing , Chalmers Zachary R. , Newberg Justin , Kim Eejung , Carrot-Zhang Jian , Piou Daphnee , Polak Paz , Abdulkadir Sarki A. , Ziv Elad , Meyerson Matthew , Frampton Garrett M. , Campbell Joshua D. , Huang Franklin W.
Published: July 2020

Purpose:

African American (AFR) men have the highest mortality rate from prostate cancer (PCa) compared with men of other racial/ancestral groups. Differences in the spectrum of somatic genome alterations in tumors between AFR men and other populations have not been well-characterized due to a lack of inclusion of significant numbers in genomic studies.

Experimental Design:

To identify genomic alterations associated with race, we compared the frequencies of somatic alterations in PCa obtained from four publicly available datasets comprising 250 AFR and 611 European American (EUR) men and a targeted sequencing dataset from a commercial platform of 436 AFR and 3018 EUR men.

Results:

Mutations in ZFHX3 as well as focal deletions in ETV3 were more frequent in tumors from AFR men. TP53 mutations were associated with increasing Gleason score. MYC amplifications were more frequent in tumors from AFR men with metastatic PCa, whereas deletions in PTEN and rearrangements in TMPRSS2-ERG were less frequent in tumors from AFR men. KMT2D truncations and CCND1 amplifications were more frequent in primary PCa from AFR men. Genomic features that could impact clinical decision making were not significantly different between the two groups including tumor mutation burden, MSI status, and genomic alterations in select DNA repair genes, CDK12, and in AR.

Conclusions:

Although we identified some novel differences in AFR men compared with other populations, the frequencies of genomic alterations in current therapeutic targets for PCa were similar between AFR and EUR men, suggesting that existing precision medicine approaches could be equally beneficial if applied equitably.
A revision of the French Telothelepodidae and Thelepodidae (Annelida, Terebelliformia), with descriptions of three species and first European record of a non-indigenous species
Authors: LAVESQUE NICOLAS , LONDOÑO-MESA MARIO H. , DAFFE GUILLEMINE , HUTCHINGS PAT
Published: July 2020

Telothelepodidae and Thelepodidae from French waters are revised based on material available in French marine stations and newly collected specimens. This work is the second part of the Spaghetti Project aiming to revise French species of “Spaghetti” worms. It describes three new species using both morphological and molecular tools: Streblosoma cabiochi n. sp., Streblosoma lindsayae n. sp. and Thelepus corsicanus n. sp. This study also permitted us to detect the presence of an Asiatic species, Thelepus japonicus, in Arcachon Bay and Normandy, introduced via oysters transfers. An identification key for European species of both families is also provided.
Changes in inflammatory biomarkers are related to the antidepressant effects of Ayahuasca
Authors: Galvão-Coelho Nicole Leite , de Menezes Galvão Ana Cecília , de Almeida Raíssa Nóbrega , Palhano-Fontes Fernanda , Campos Braga Isaac , Lobão Soares Bruno , Maia-de-Oliveira João Paulo , Perkins Daniel , Sarris Jerome , de Araujo Draulio Barros
Published: July 2020

Background:

Ayahuasca is a traditional Amazon brew and its potential antidepressant properties have recently been explored in scientific settings. We conducted a double-blind placebo-controlled trial of ayahuasca with treatment-resistant depression patients ( n = 28) and healthy controls ( n = 45).

Aims:

We are evaluating the blood inflammatory biomarkers: C-reactive protein and interleukin 6, as a potential consequence of ayahuasca intake and their correlation with serum cortisol and brain-derived neurotrophic factor levels. Blood samples were collected at pre-treatment and 48 hours after substance ingestion to assess the concentration of inflammatory biomarkers, together with administration of the Montgomery-Åsberg Depression Rating Scale.

Results:

At pre-treatment, patients showed higher C-reactive protein levels than healthy controls and a significant negative correlation between C-reactive protein and serum cortisol levels was revealed ( rho = –0.40, n = 14). C-reactive protein in those patients was not correlated with Montgomery-Åsberg Depression Rating Scale scores. We observed a significant reduction of C-reactive protein levels across time in both patients and controls treated with ayahuasca, but not with placebo. Patients treated with ayahuasca showed a significant correlation ( rho = + 0.57) between larger reductions of C-reactive protein and lower depressive symptoms at 48 hours after substance ingestion (Montgomery-Åsberg Depression Rating Scale). No significant result with respect to interleukin 6 and brain-derived neurotrophic factor was found. Furthermore, these biomarkers did not predict the antidepressant response or remission rates observed.

Conclusions:

These findings enhance the understanding of the biological mechanisms behind the observed antidepressant effects of ayahuasca and encourage further clinical trials in adults with depression.
Understanding match-fixing from the perspective of social capital: A case study of Taiwan’s professional baseball system
Authors: Tzeng Chien-Chun , Lee Ping-Chao
Published: July 2020

The present study aims to explore the social conditions and underlying mechanism of match-fixing, especially when it is not facilitated in a quid pro quo relationship. Analyzing scandals in Taiwan’s Chinese Professional Baseball League, our research investigates how both individual agendas and organizational interests are served in match-fixing. Practically, the prerequisites and actors’ rationales for their involvement in match-fixing are explained. Theoretically, actors’ motivations and the exchange of intangible resources (not in the form of money) are examined. Our findings speak to theories of structural, cognitive and relational social capital by elaborating on the dark side of social capital. The relational attributes possessed by actors embedded in their social networks are involved in the cognitive process of actors’ decision making. Alternative policy implications are proposed to reduce the potential for match-fixing.
Detecting Local Clusters of Under-5 Malnutrition in the Province of Marinduque, Philippines Using Spatial Scan Statistic
Authors: Leyso Novee Lor C , Palatino Maylin C
Published: July 2020

Underweight and overweight among under-5 children continue to persist in the island Province of Marinduque, Philippines. Local spatial cluster detection provides a spatial perspective in understanding this phenomenon, specifically in which areas the double burden of malnutrition occurs. Using data from a province-wide census conducted in 2014-2016, we aimed to identify spatial clusters of different forms of malnutrition in the province and determine its relative risk. Weight-for-age z score was used to categorize the malnourished children into severely underweight, moderately underweight, and overweight. We used the multinomial model of Kulldorff’s elliptical spatial scan statistic, adjusting for age and socioeconomic status. Four significant clusters across municipalities of Boac, Buenavista, Gasan, and Torrijos were found to have high risk of overweight and underweight simultaneously, indicating existence of double burden of malnutrition within these communities. These clusters should be targeted with tailored plans to respond to malnutrition, at the same time maximizing the resources and benefits.
A quantitative assessment of the parameters of the role of receptionists in modern primary care using the work design framework
Authors: Burrows Michael , Gale Nicola , Greenfield Sheila , Litchfield Ian
Published: July 2020

Background

Amidst increased pressures on General Practice across England, the receptionist continues to fulfil key administrative and clinically related tasks. The need for more robust support for these key personnel to ensure they stay focussed and motivated is apparent, however, to be effective a more systematic understanding of the parameters of their work is required. Here we present a valuable insight into the tasks they fulfil, their relationship with colleagues and their organisation and their attitudes and behaviour at work collectively defined as their ‘work design’.

Methods

Our aim was to quantitatively assess the various characteristics of receptionists in primary care in England using the validated Work Design Questionnaire (WDQ) a 21 point validated questionnaire, divided into four categories: task, knowledge and social characteristics and work context with a series of sub-categories within each, disseminated online and as a postal questionnaire to 100 practices nationally.

Results

Seventy participants completed the WDQ, 54 online and 16 using the postal questionnaire with the response rate for the latter being 3.1%. The WDQ suggested receptionists experience high levels of task variety, task significance and of information processing and knowledge demands, confirming the high cognitive load placed on receptionists by performing numerous yet significant tasks. Perhaps in relation to these substantial responsibilities a reliance on colleagues for support and feedback to help negotiate this workload was reported.

Conclusion

The evidence of our survey suggests that the role of modern GP receptionists requires an array of skills to accommodate various administrative, communicative, problem solving, and decision-making duties. There are ways in which the role might be better supported for example devising ways to separate complex tasks to avoid the errors involved with high cognitive load, providing informal feedback, and perhaps most importantly developing training programmes.
The protective effect of 3% diquafosol on meibomian gland morphology in glaucoma patients treated with prostaglandin analogs: a 12-month follow-up study
Authors: Guo Yue , Ha Jun Young , Piao He Long , Sung Mi Sun , Park Sang Woo
Published: July 2020

Background

To determine if 3% diquafosol (DQS) can preserve the meibomian gland morphology in glaucoma patients treated with prostaglandin analogs (PGA) for a 12-month follow-up period.

Methods

This study included 84 eyes of 46 normal tension glaucoma (NTG) patients who were treated with either preservative-containing PGA (PC-PGA; 16 patients, 28 eyes), preservative-free PGA (PF-PGA; 21 patients, 39 eyes), or a combination of PC-PGA and 3% DQS (PC-PGA + DQS; 9 patients, 17 eyes). The meibography of the upper eyelid was acquired using Keratograph® 5 M at baseline and at each follow-up (1, 3, 6, 9, and 12 months). Meibomian gland loss (MGL) was quantitatively analyzed by using ImageJ software.

Results

In the PC-PGA group, MGL increased significantly from baseline to month 9 and month 12, whereas no significant changes were observed in the PF-PGA and PC-PGA + DQS groups during the entire 12 months. All groups showed similar MGL at each follow-up time from baseline to six months. However, MGL in the PC-PGA group was significantly higher than those in the PF-PGA and PC-PGA + DQS groups at the 9 and 12 months.

Conclusions

Combining 3% DQS with PC-PGA was as effective as PF-PGA in preserving the meibomian gland morphology for at least 12 months. Our results suggest that 3% DQS may be a promising strategy for managing glaucoma patients with a high risk of developing meibomian gland dysfunction due to preservative-containing topical medications.
Quality of life of patients with metastatic pancreatic adenocarcinoma initiating first-line chemotherapy in routine practice
Authors: Laquente Berta , Macarulla Teresa , Bugés Cristina , Martín Marta , García Carlos , Pericay Carles , Merino Sandra , Visa Laura , Martín Teresa , Pedraza Manuela , Carnero Beatriz , Guardeño Raquel , Verdaguer Helena , Mut Alejandro , Vilanova David , García Adelaida
Published: July 2020

Background

Despite advances in surgery, radiotherapy, and chemotherapy, pancreatic adenocarcinoma often progresses rapidly and causes death. The physical decline of these patients is expected to impact their quality of life (QoL). Therefore, in addition to objective measures of effectiveness, the evaluation of health-related QoL should be considered a matter of major concern when assessing therapy outcomes.

Methods

Observational, prospective, multicenter study including patients with metastatic pancreatic adenocarcinoma who started first-line chemotherapy in 12 Spanish centers. Treatment and clinical characteristics were recorded at baseline. Patients’ health-related quality of life, ECOG, and Karnofsky index were measured at baseline, at Days 15 and 30, and every four weeks up to 6 months of chemotherapy. Health-related quality of life was measured using the EORTC-QLQ-C30 and EQ-5D questionnaires. Other endpoints included overall survival and progression-free survival.

Results

The study sample included 116 patients (median age of 65 years). Mean (SD) scores for the QLQ-C30 global health status scale showed a significant increasing trend throughout the treatment (p = 0.005). Patients with either a Karnofsky index of 70–80 or ECOG 2 showed greater improvement in the QLQ-C30 global health status score than the corresponding groups with better performance status (p ≤ 0.010). Pain, appetite, sleep disturbance, nausea, and constipation significantly improved throughout the treatment (p < 0.005). Patients with QLQ-C30 global health status scores ≥50 at baseline had significantly greater overall survival and progression-free survival (p = 0.005 and p = 0.021, respectively). No significant associations were observed regarding the EQ-5D score.

Conclusions

Most metastatic pancreatic adenocarcinoma patients receiving first-line chemotherapy showed an increase in health-related quality of life scores throughout the treatment. Patients with lower performance status and health-related quality of life at baseline tended to greater improvement. The EORTC QLQ-C30 scale allowed us to measure the health-related quality of life of metastatic pancreatic adenocarcinoma patients receiving first-line chemotherapy.
Usefulness of speckle-tracking echocardiography for early detection in children with Duchenne muscular dystrophy: a meta-analysis and trial sequential analysis
Authors: Song Guang , Zhang Jing , Wang Xin , Zhang Xintong , Sun Feifei , Yu Xiaona
Published: July 2020

Background

Duchenne muscular dystrophy (DMD) is the most common form of inherited muscle disease in children. The incidence of cardiomyopathy induced by DMD increases with age. Left ventricular ejection fraction usually fails to reflect the subclinical left ventricular dysfunction. Several studies have assessed this dysfunction using myocardial strain measured by speckle-tracking echocardiography (STE). However, the results were inconsistent and incomplete.

Methods

Several databases were searched from their inception to February 5, 2020. The summarized weighted mean difference (WMD) with 95% confidence intervals (CIs) were estimated for myocardial strain between DMD and healthy controls and a meta-analysis was conducted. Trial sequential analysis estimated whether the resulting evidence was sufficient.

Results

Eight studies with a total of 269 DMD children and 299 healthy participants were included. STE revealed that global longitudinal strain (GLS), global circumferential strain, average longitudinal strain (measured by two-dimensional STE at the apical four-chamber view), and average circumferential strain (measured by two-dimensional STE at the papillary muscle short-axis level) decreased (WMD = 4.17, 95% CI: 3.03–5.32; WMD = 3.98, 95% CI: 0.29–7.68; WMD = 4.18, 95% CI: 2.75–5.62; and WMD = 4.90, 95% CI: 2.38–7.43, respectively; all P < 0.05) compared with the controls and global radial strain was unchanged in the DMD group (WMD = − 4.33, 95% CI: − 9.53–0.87, P = 0.103). Trial sequential analysis indicated that available GLS samples were sufficient and confirmed that adequate evidence was accumulated. The credibility of other myocardial strains was questioned due to insufficiently involved studies.

Conclusion

GLS can be useful for early detection of left ventricle myocardial dysfunction in children with DMD.
Physical activity-mediated associations between perceived neighborhood social environment and depressive symptoms among Jackson Heart Study participants
Authors: Tamura Kosuke , Langerman Steven D. , Orstad Stephanie L. , Neally Sam J. , Andrews Marcus R. , Ceasar Joniqua N. , Sims Mario , Lee Jae E. , Powell-Wiley Tiffany M.
Published: July 2020

Background

Little is known about the associations between perceived neighborhood social environment (PNSE) and depressive symptoms among African Americans. Furthermore, the role of physical activity (PA) as a mediator of this association has not been investigated. The two-fold objectives of this study, therefore, were (1) to examine the associations between PNSE and depressive symptoms among African Americans, and (2) to test the degree to which these associations were mediated by total PA.

Methods

We used baseline data from the Jackson Heart Study (JHS), a single-site, prospective, community-based study of African-American adults (n = 2209) recruited from Jackson, Mississippi. PNSE variables included scores for neighborhood violence (i.e., higher score = more violence), problems (higher score = more problems), and social cohesion (higher score = more cohesion). Depressive symptoms were measured by the 20-item Center for Epidemiological Studies Depression (CES-D) score. First, multilevel modeling, controlling for census tract clustering effects, was used to estimate associations between each PNSE variable and CES-D score, adjusting for covariates, including demographic, health-related, and population density. Second, validated, self-reported total PA, based on active living, sport, and home indices, was tested as the mediator. Multivariable linear regressions with bootstrap-generated 95% bias-corrected confidence intervals (BC CIs) were estimated to test for significant unstandardized indirect effects, controlling for all covariates.

Results

Our participants were 64.2% female with a mean age of 52.6 (SD = 12.2) and a mean CES-D score of 10.8 (SD = 8.1). In the fully-adjusted model, neighborhood violence and problems were positively related to depressive symptoms (B = 3.59, 95%CI = 0.93, 6.26, and B = 3.06, 95%CI = 1.19, 4.93, respectively). Neighborhood violence and problems were also indirectly related to depressive symptoms via total PA (B = 0.26, 95%BC CI = 0.05, 0.55; and B = 0.15, 95%BC CI = 0.02, 0.34, respectively). Social cohesion was neither directly nor indirectly related to depressive symptoms.

Conclusions

We found that higher levels of perceived neighborhood problems and violence were directly and positively associated with depressive symptoms. These associations may be explained in part by lower total PA levels. Future interventions to reduce depressive symptoms attributed to neighborhood features should consider emphasizing built environment features that facilitate PA increases in conjunction with community efforts to reduce neighborhood violence and problems.
CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons
Authors: Chang Guo-Qing , Karatayev Olga , Boorgu Devi Sai Sri Kavya , Leibowitz Sarah F.
Published: July 2020

Background

Clinical and animal studies show that alcohol consumption during pregnancy produces lasting behavioral disturbances in offspring, including increased alcohol drinking, which are linked to inflammation in the brain and disturbances in neurochemical systems that promote these behaviors. These include the neuropeptide, melanin-concentrating hormone (MCH), which is mostly expressed in the lateral hypothalamus (LH). Maternal ethanol administration at low-to-moderate doses, while stimulating MCH neurons without affecting apoptosis or gliogenesis, increases in LH the density of neurons expressing the inflammatory chemokine C-C motif ligand 2 (CCL2) and its receptor CCR2 and their colocalization with MCH. These neural effects associated with behavioral changes are reproduced by maternal CCL2 administration, reversed by a CCR2 antagonist, and consistently stronger in females than males. The present study investigates in the embryo the developmental origins of this CCL2/CCR2-mediated stimulatory effect of maternal ethanol exposure on MCH neurons.

Methods

Pregnant rats from embryonic day 10 (E10) to E15 during peak neurogenesis were orally administered ethanol at a moderate dose (2 g/kg/day) or peripherally injected with CCL2 or CCR2 antagonist to test this neuroimmune system’s role in ethanol’s actions. Using real-time quantitative PCR, immunofluorescence histochemistry, in situ hybridization, and confocal microscopy, we examined in embryos at E19 the CCL2/CCR2 system and MCH neurons in relation to radial glia progenitor cells in the hypothalamic neuroepithelium where neurons are born and radial glia processes projecting laterally through the medial hypothalamus that provide scaffolds for neuronal migration into LH.

Results

We demonstrate that maternal ethanol increases radial glia cell density and their processes while stimulating the CCL2/CCR2 system and these effects are mimicked by maternal administration of CCL2 and blocked by a CCR2 antagonist. While stimulating CCL2 colocalization with radial glia and neurons but not microglia, ethanol increases MCH neuronal number near radial glia cells and making contact along their processes projecting into LH. Further tests identify the CCL2/CCR2 system in NEP as a primary source of ethanol’s sexually dimorphic actions.

Conclusions

These findings provide new evidence for how an inflammatory chemokine pathway functions within neuroprogenitor cells to mediate ethanol’s long-lasting, stimulatory effects on peptide neurons linked to adolescent drinking behavior.
Socio-demographic factors of cesarean births in Nha Trang city, Vietnam: a community-based survey
Authors: Takegata Mizuki , Ronsmans Carine , Nguyen Hien Anh T. , Kitamura Noriko , Iwasaki Chihiro , Toizumi Michiko , Moriuchi Hiroyuki , Dang Duc Anh , Yoshida Lay-Myint
Published: July 2020

Background

The cesarean section rate in Vietnam has been increasing especially in urban area. However, limited evidence identified regarding socio-demographic factors of the cesarean section birth. The objective of this study was to determine the current cesarean birth rate and the associated socio-demographic factors among mothers in Nha Trang city, south-central Vietnam.

Methods

A community-based cross-sectional study was conducted between October and November in 2016 as part of a Streptococcus pneumoniae carriage survey conducted in 27 communes of Nha Trang city. From each commune, 120 mothers and their children less than 2 years old were randomly selected. Mothers were asked to answer standardized questions regarding socio-demographic information and mode of birth. Multivariate logistic regression was adopted to examine associations between socio-demographic variables and mode of birth.

Results

Of 3148 participants, the number of cesarean births was 1396 (44.3 %). Older maternal age (≥ 30 years old), having another child going to school or kindergarten, monthly income more than 644 USD, gestational weeks at birth over 42 weeks, and low (< 2500 g) or high (≥ 3500 g) birth weight were associated with higher likelihood of cesarean births.

Conclusion

The CS rate obtained in this study was more than twice of what is recommended by the World Health Organization, which is consistent with the previous nation-wide study in Viet Nam. Further monitoring is suggested to examine the non-medical reason for the increased CS rate.
Multiparametric quantitative analysis of tumor perfusion and diffusion with 3T MRI: differentiation between benign and malignant soft tissue tumors
Authors: Lee Seul Ki , Jee Won-Hee , Jung Chan Kwon , Chung Yang-Guk
Published: July 2020

Objective:

To evaluate multiparametric MRI for differentiating benign and malignant soft tissue tumors.

Methods:

This retrospective study included 67 patients (mean age, 55 years; 18–82 years) with 35 benign and 32 malignant soft tissue tumors. Intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI)-derived parameters (D, D*, f), apparent diffusion coefficient (ADC), and dynamic contrast-enhanced (DCE)-MRI parameters (Ktrans, Kep, Ve, iAUC) were calculated. Myxoid and non-myxoid soft tissue tumors were divided for subgroup analysis. The parameters were compared between benign and malignant tumors.

Results:

ADC and D were significantly lower in malignant than benign soft tissue tumors (1170 ± 488 vs 1472 ± 349 µm2/s; 1132 ± 500 vs 1415 ± 374 µm2/s; p < 0.05). Ktrans, Kep, Ve, and iAUC were significantly different between malignant and benign soft tissue tumors (0.209 ± 0.160 vs 0.092 ± 0.067 min−1; 0.737 ± 0.488 vs 0.311 ± 0.230 min−1; 0.32 ± 0.17 vs 0.44 ± 0.28; 0.23 ± 0.14 vs 0.12 ± 0.09, p < 0.05, respectively). ADC (0.752), D (0.742), and Kep (0.817) had high AUCs. Subgroup analysis showed that only Ktrans, and iAUC were significantly different in myxoid tumors, while, ADC, D, Ktrans, Kep, and iAUC were significantly different in non-myxoid tumor for differentiating benign and malignant tumors. D, Kep, and iAUC were the most significant parameters predicting malignant soft tissue tumors.

Conclusion:

Multiparametric MRI can be useful to differentiate benign and malignant soft tissue tumors using IVIM-DWI and DCE-MRI.

Advances in knowledge:

1. Pure tissue diffusion (D), transfer constant (Ktrans), rate constant (Kep), and initial area under time–signal intensity curve (iAUC) can be used to differentiate benign malignant soft tissue tumors. 2. Ktrans and iAUC enable differentiation of benign and malignant myxoid soft tissue tumors.
BAME staff and microaggressions
Authors: Foster Sam
Published: July 2020

Sam Foster, Chief Nurse, Oxford University Hospitals, considers the role of reverse mentoring in her approach to being a BAME ambassador
Qualitative behaviour assessment as an indicator of animal emotional welfare in farm assurance
Authors: Cooper Richard , Wemelsfelder Françoise
Published: July 2020

Acknowledgement of animals as sentient beings (including farm animal species), capable of experiencing positive and negative emotions, has highlighted the need for suitable ‘welfare outcome’ measures in farm assurance schemes. Current schemes tend to focus on measures of physical health and productivity, but there is as yet a lack of indicators addressing farmed animals' emotional wellbeing. A number of assessment techniques exist that may help us develop such indicators, and better understand the extent to which farm animals do, or do not, experience ‘a good life’. This article focuses on one such technique, ‘qualitative behaviour assessment’ (QBA), and reviews the potential benefits of, and challenges to, its practical deployment in the field.