bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Bamboo on Rainfall-Triggered Landslide Distribution at the Regional Scale: A Case Study from SE China
Authors: Guo Zizheng , Guo Zhanxu , Wen Chunchun , Xu Gang , Zhang Yuhua , Zhang Hao , Qin Haiyan , Zhang Yuzhi , He Jun
Published: December 2024

It is widely accepted that land use and land cover (LULC) is an important conditioning factor for landslide occurrence, especially when considering the role of tree roots in stabilizing slopes and consolidating the soil. However, it is still difficult to assess the impacts of a specific LULC type on landslide distribution. The objective of the present study is to reveal the relationship between bamboo and landslide distribution at the regional scale. We aim to answer the following question: do the areas covered by bamboo have a higher susceptibility to landslides? Wenzhou City in SE China was taken as the study area, and a landslide inventory containing 1725 shallow landslides was constructed. The generalized additive model (GAM) was employed to assess the significance of LULC and nine additional factors, all of which were generated using the GIS platform. The frequency ratio (FR) method was used to analyze and compare the landslide density in each LULC category. Machine learning models were applied to perform landslide susceptibility mapping of the region. The results show that in the Wenzhou region, LULC is the second most important factor for landslide occurrences after the slope factor, whereas bamboo has a relatively higher FR value than most other LULC categories. The accuracies of the landslide susceptibility maps obtained from the random forest and XGBoost models were 79.6% and 85.3%, respectively. Moreover, 23.8% and 25.5% of the bamboos were distributed in very-high- and high-susceptibility-level areas. The incidents and density of landslides in bamboo areas were significantly higher than those with debris flow and rock collapses, indicating a promotional effect of bamboo on slope failure in the study area. This work will improve our understanding regarding the role of geological and ecological conditions that affect slope stability, which may provide useful guidance for land use planning and landslide risk assessment and mitigation at the regional scale.
Analysis of CH4 and N2O Fluxes in the Dry Season: Influence of Soils and Vegetation Types in the Pantanal
Authors: Cugler Gabriela , Figueiredo Viviane , Gauci Vincent , Stauffer Tainá , Peixoto Roberta Bittencourt , Rao Pangala Sunitha , Enrich-Prast Alex
Published: December 2024

This study examines CH4 and N2O fluxes during the dry season in two distinct areas of the Pantanal: Barranco Alto Farm (BAF), dominated by grasslands, and Passo da Lontra (PL), a forested region. As climate change increases the occurrence of droughts, understanding greenhouse gas (GHG) fluxes in tropical wetlands during dry periods is crucial. Using static chambers, CH4 and N2O emissions were measured from soils and tree stems in both regions, with additional measurements from grass in BAF. Contrary to expectations, PL—characterized by clayey soils—had sandy mud samples that retained less water, promoting oxic conditions and methane uptake, making it a CH4 sink. Meanwhile, BAF’s sandy, well-drained soils exhibited minimal CH4 fluxes, with negligible methane uptake or emissions. N2O fluxes were generally higher in BAF, particularly from tree stems, indicating significant interactions between soil type, moisture, and vegetation. These findings highlight the pivotal roles of soil texture and aeration in GHG emissions, suggesting that well-drained, sandy soils in tropical wetlands may not always enhance methane oxidation. This underscores the importance of continuous GHG monitoring in the Pantanal to refine climate change mitigation strategies.
The Role of Ecological Space Structure Optimization in Synergetic Sustainable Development: Evidence from the Guangdong–Hong Kong–Macao Greater Bay Area
Authors: Fan Deliang , Zeng Xiaoyue , Wang Heyang , Wang Meixue , Li Qiuyao , Jiang Haiyan , Li Shijie
Published: December 2024

Improving carbon sink capacity is critical for meeting energy conservation and emission reduction targets, along with low-carbon development goals. Although many researchers have recognized that urban space can significantly influence the capacity of carbon sinks in urban areas, few studies have quantified the impact of urban landscape patterns, particularly urban green space (UGS) morphology, on carbon sinks. This study quantitatively investigated the impact of UGS morphology on carbon sinks using panel data from nine cities in the Guangdong–Hong Kong–Macao Greater Bay Area (GBA), China, from 2000 to 2017. A series of landscape metrics and land-use data was first selected to quantify the four dimensions of UGS: scale, compactness, type diversity, and shape complexity. Subsequently, the impact of UGS morphology on carbon sinks was estimated using a panel data model. The main findings were as follows. (1) From the carbon sink results, the carbon sink in the GBA was found to be in an evolutionarily stable state, reflecting the relatively limited potential for carbon enhancement in the region. (2) From the landscape metrics results, the AREA_MD was mainly distributed between 0.18 and 0.27. The AI was mainly distributed between 80 and 97, with a concentration at 90. The PAFRAC was mainly distributed between 1.39 and 1.43, with a concentration at 1.41. PR was mainly distributed between 6 and 7. There was a large disparity in the scale of UGS morphology between GBA cities, with an irregular and complex pattern that became more compact over time. (3) From the panel data results, the AI (p < 0.001) had a significant positive impact on carbon sinks, while PR (p < 0.001) had a significant negative impact. The more compact the UGS morphology, the greater the total carbon sink, and the more complex the UGS morphology, the smaller the total carbon sink. The findings highlighted the importance of urban planning and UGS morphological optimization for increasing carbon sink capacity, as well as providing policy makers and urban planners with theoretical references and guidance for achieving climate goals.
Improvement in Palm Kernel Meal Quality by Solid-Sate Fermentation with Bacillus velezensis, Saccharomyces cerevisiae and Lactobacillus paracasei
Authors: Zhu Xiaoyan , Deng Zechen , Wang Qiuyue , Hao Shanxin , Liu Pei , He Shuai , Li Xiangqian
Published: December 2024

To improve the quality of palm kernel meal (PKM), the effect of solid-state fermentation (SSF) with Bacillus velezensis, Saccharomyces cerevisiae and Lactobacillus paracasei on nutritional components, anti-nutritional factor and antioxidant activity were investigated. The results show that inoculation ratio of three strains 4:2:1, inoculation amount 21%, moisture content 52%, fermentation temperature 34 °C and fermentation time 60 h were the optimal SSF conditions. After 60 h of fermentation, the content of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose and hemicellulose in PKM were significantly decreased by 22.5%, 18.2%, 20.2%, 17.6% and 32.4%, respectively. Meanwhile, the content of crude protein, soluble protein, peptides, amino acids and reducing sugar were increased significantly by 27.3%, 193%, 134%, 16.3% and 228%, respectively. SSF significantly improved the total phenolic content, DPPH radical scavenging activity, hydroxyl radical scavenging activity and reducing power. In addition, in vitro dry matter digestibility (IVDMD) and in vitro crude protein digestibility (IVCPD) were increased. Scanning electron microscopy (SEM) analysis revealed microstructural alterations in PKM. The results indicate that SSF with B. velezensis, S. cerevisiae and L. paracasei is an effective and promising method to enhance the nutritional value and antioxidant activity of PKM, providing a feasible solution for increasing the utilization of PKM in animal feed.
Impact of Defects on Tensile Properties of Ancient and Modern Egyptian Flax Fibers: Multiscale X-Ray Microtomography and Numerical Modeling
Authors: Rajakumaran Vasuki , Guessasma Sofiane , D’Orlando Angélina , Melelli Alessia , Scheel Mario , Weitkamp Timm , Perrin Jonathan , Bourmaud Alain , Proudhon Henry , Beaugrand Johnny
Published: December 2024

Flax fibers, while offering numerous benefits, are susceptible to mechanical weakening due to the presence of kink-bands within their structure. The novelty of this study lies in linking mechanical behavior to fiber morphology and defects at multiple scales by utilizing X-ray microtomography to generate detailed 3D images of elementary flax fibers, enabling the creation of accurate finite element (FE) models for analysis. Aging reduces flax fibers’ strength, so both modern and ancient fibers were analyzed to understand their structural evolution over time. Static X-ray microtomography images were converted into 3D FE models for tensile simulations, and tensile tests provided essential properties for numerical modeling. Morphological analysis for both fiber types revealed that kink-bands contain multiple pores oriented ~45° to the fiber/lumen axis, with ancient fibers showing higher porosity (5.6%) and kink-band density (20.8 mm⁻¹) than modern fibers (3.3% and 16.6 mm⁻¹). SEM images confirmed that the intricate lumen and kink-bands lead to fiber failure under tensile loading. Numerical analysis highlighted higher stress concentrations at the kink-band region, particularly at pores in the kink-band region, which can initiate cracks and lead to rupture.
Computational Modeling of Biomass Fast Pyrolysis in Fluidized Beds with Eulerian Multifluid Approach
Authors: Venier Cesar M. , Torres Erick , Fouga Gastón G. , Rodriguez Rosa A. , Mazza Germán , Reyes Urrutia Andres
Published: December 2024

This study investigated the fast pyrolysis of biomass in fluidized-bed reactors using computational fluid dynamics (CFD) with an Eulerian multifluid approach. A detailed analysis was conducted on the influence of various modeling parameters, including hydrodynamic models, heat transfer correlations, and chemical kinetics, on the product yield. The simulation framework integrated 2D and 3D geometrical setups, with numerical experiments performed using OpenFOAM v11 and ANSYS Fluent v18.1 for cross-validation. While yield predictions exhibited limited sensitivity to drag and thermal models (with differences of less than 3% across configurations and computational codes), the results underline the paramount role of chemical kinetics in determining the distribution of bio-oil (TAR), biochar (CHAR), and syngas (GAS). Simplified kinetic schemes consistently underestimated TAR yields by up to 20% and overestimated CHAR and GAS yields compared to experimental data (which is shown for different biomass compositions and different operating conditions) and can be significantly improved by redefining the reaction scheme. Refined kinetic parameters improved TAR yield predictions to within 5% of experimental values while reducing discrepancies in GAS and CHAR outputs. These findings underscore the necessity of precise kinetic modeling to enhance the predictive accuracy of pyrolysis simulations.
The Effects of Cooking Methods on Gel Properties, Lipid Quality, and Flavor of Surimi Gels Fortified with Antarctic Krill (Euphausia superba) Oil as High Internal Phase Emulsions
Authors: Lv Yinyin , Wang Xiuqin , Hao Ruoyi , Zhang Xianhao , Xu Xianbing , Li Shengjie , Dong Xiuping , Pan Jinfeng
Published: December 2024

In this study, silver carp surimi products enriched with Antarctic krill oil high internal phase emulsions (AKO-HIPEs) were cooked using steaming (STE), microwave heating (MIC), and air-frying (AIR), respectively. The gel and flavor properties, lipid quality and stability were investigated. Compared to the MIC and AIR groups, the STE surimi gel added with HIPEs had better texture properties, exhibiting higher water-holding capacity and a more homogeneous structure, while the air-frying treatment resulted in visually brighter surimi products. The degree of lipid oxidation during cooking was in an order of STE < MIC < AIR as determined by electron paramagnetic resonance spectrometer and thiobarbituric acid reactive substances. HIPE-added surimi gels retained more nutrients and flavor when cooked by AIR compared to STE and MIC. Results imply that the texture properties and lipid stability of surimi products fortified with AKO-HIPEs were better than those of the oil group under any cooking method. In conclusion, surimi products added with AKO-HIPEs had better gel properties and retained more fatty acids and flavor than AKO-SO.
Indirect Fortification of Traditional Nixtamalized Tortillas with Nixtamalized Corn Flours
Authors: Nieves-Hernandez María Guadalupe , Correa-Piña Brenda Lizbeth , Garcia-Chavero Oscar , Lopez-Ramirez Salomon , Florez-Mejia Rosendo , Barrón-García Oscar Yael , Gutierrez-Cortez Elsa , Gaytán-Martínez Marcela , Rojas-Molina Juana Isela , Rodriguez-Garcia Mario E.
Published: December 2024

Background: This work focused on the study of the indirect fortification of Mexican tortillas made from nixtamalized masa (NM) with nixtamalized commercial corn flour (NCC-F) fortified with Zn, Fe, vitamins and folic acid. Methods: The chemical proximate values (CPV), ash content, mineral composition by inductively coupled plasma, in vitro protein digestibility (PD), protein digestibility-corrected amino acid score (PDCAAS), the total starch content, the resistant starch (RS) content in nixtamalized corn tortillas (NC-T) and nixtamalized commercial corn flour tortillas (NCCF-T) and the contribution of tortillas prepared with a mixture of NM and NCC-F (75:25 and 50:50, NM:NCC-F) to the recommended dietary intake (RDI) of minerals and vitamins were determined. Results: No significant differences (p < 0.05) were found in CPV and RS content between NCCF-T and NC-T. Ca content was significantly higher (p < 0.05) in NC-T than in NCCF-T, while Fe, K, Zn, folic acid contents, PD and PDCAAS content was higher in NCCF-T compared to NC-T (p < 0.05). The tortillas made with a mixture of NM and NCC-F (50:50) provide 43.07% of the RDI of Ca for Mexican children and adults, while ~45% and >100% of the RDI of Mg for adults and children, respectively, are provided by these tortillas. Similarly, tortillas from the NM:NCC-F mixture (50:50) provide average values of 45, 71 and ~91% of the RDI of Fe, Zn and folic acid, respectively, for all age groups of the Mexican population. Conclusions: NCCF-T contribute significantly to the recommended daily intake (RDI) of micronutrients such as iron, zinc, magnesium and folic acid, while tortillas made from the traditional nixtamalized corn of the Mexican diet have a higher calcium content. Tortillas made from a mixture of NCC-F and traditional NM may be an effective way to address micronutrient deficiencies in the Mexican population.
Oat Kilning and Its Effects on Liquid Oat-Base Production
Authors: Ekelund Sofie , Gladkauskas Eimantas , Krause Svenja , Sirijovski Nick , Adlercreutz Patrick , Rayner Marilyn , Grey Carl , Tullberg Cecilia
Published: December 2024

The aim of this study was to give insights on the effects of an industrially relevant kilning method, with a focus on lipase inactivation and oat-base production. Storage of non-kilned, dehulled oat kernels in either room temperature or at 37 °C for up to 64 days led to increasing lipase activity with time, despite a decrease in moisture content and water activity, demonstrating the importance of kilning before storage. It was shown that the temperature and relative humidity used during the kilning had a major impact on both protein solubility and lipase inactivation. Steaming with saturated steam at 90 °C for 30 min followed by drying at 100 °C for 30 min was found to be enough to inactivate all lipases but still leave a relatively high protein solubility (42% of the total soluble protein). In the sensory trial, the indication was that kilning was a vital step from a sensory quality perspective, giving a pleasant aroma to the final oat-base product. However, with kilning, the final protein content decreased; thus, these results are important for the development of future oat-base products, where quality, nutritional content, and taste need to be balanced.
Efficacy of Hovenia dulcis Fruit Extract in Hangover Mitigation: Double-Blind Randomized Clinical Evaluation
Authors: Paik Dong Hyun , Lee Ki Won , Shim Youn Young , Reaney Martin J. T. , Park Ilbum , Lee Sang-Hun , Park Jong-Yul , Park Euddeum , Lee Sung-Bum , Kim In Ah , Xu Guangpeng , Hong Ji Youn , Kim Young Jun
Published: December 2024

The fruit of Hovenia dulcis Thunb. (HD) is renowned for its medicinal properties and is rich in bioactive compounds, traditionally used in East Asian medicine as a natural antidote for alcohol intoxication. A randomized, double-blind, crossover, placebo (PLA)-controlled clinical trial was conducted to compare the effects of beverages containing 0.475% HD (HDB), HD combined with 0.1% Pueraria lobata extract (HDPB), and HD combined with 0.02% glutathione yeast extract (HDGB) with the PLA on the symptoms of a hangover. Subjects (n = 30) were randomized into six test groups consuming three beverages, including the PLA. After ingestion, blood alcohol and blood acetaldehyde concentrations were measured at 0, 0.25, 0.5, 1, 2, 4, 6, and 15 h post-alcohol consumption. No significant differences in hematology tests and vital signs were observed amongst the treatment groups; however, at 0.5 and 6 h, the blood alcohol concentrations of the HDB and HDPB groups were significantly lower compared to the PLA group (p < 0.05). Additionally, the blood acetaldehyde concentrations in the HDPB group showed significantly lower values than the PLA after 6 h (p < 0.05). These findings suggest that HD may aid in alcohol detoxification and limit acetaldehyde accumulation. This indicates the potential for HD as a functional food ingredient for alleviating hangover symptoms.
Classification of Plant-Based Drinks Based on Volatile Compounds
Authors: Papp Zsigmond , Nemeth Laura Gabriela , Nzetchouang Siyapndjeu Sandrine , Bufa Anita , Marosvölgyi Tamás , Gyöngyi Zoltán
Published: December 2024

The increasing popularity of plant-based drinks has led to an expanded consumer market. However, available quality control technologies for plant-based drinks are time-consuming and expensive. Two alternative quality control methods, gas chromatography with ion mobility spectrometry (GC-IMS) and an electronic nose, were used to assess 111 plant-based drink samples. Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to compare 58 volatile organic compound areas of GC-IMS gallery plots and 63 peptide sensors of the electronic nose. PCA results showed that GC-IMS was only able to completely separate one sample, whereas the electronic nose was able to completely separate seven samples. LDA application to GC-IMS analyses resulted in classification accuracies ranging from 15.4% to 100%, whereas application to electronic nose analyses resulted in accuracies ranging from 96.2% to 100%. Both methods were useful for classification, but each had drawbacks, and the electronic nose performed slightly better than GC-IMS. This study represents one of the first studies comparing GC-IMS and an electronic nose for the analysis of plant-based drinks. Further research is necessary to improve these methods and establish a rapid, cost-effective food quality control system based on volatile organic compounds.
Human Identification in Mass Disasters: Analyzing Complex Tattoos in the Brumadinho Tragedy
Authors: Furtado Alexandre Neves , Dionísio Alexander Santos , Araújo Ricardo Moreira , Lemos Yara Vieira
Published: December 2024

Background: The identification of victims in mass disasters is a challenging task, particularly when forensic teams must address fragmented human remains. Objectives: This article reports two necropsy cases from the 2019 collapse of the Brumadinho mine tailing dam, the largest humanitarian disaster in Brazilian history. Results: In both cases, identification was achieved through comparative anthropological analysis of complex tattoos. Positive identification was based on the presence of multiple coincident points between postmortem (PM) and antemortem (AM) photographic records of the tattoo designs, along with the absence of exclusionary elements. Conclusion: The authors propose that the comparative analysis of PM and AM tattoos should be more widely adopted as a low-cost, rapid identification method, particularly in complex forensic scenarios such as mass disasters and other challenging cases in the medical and forensic anthropology context.
Strategic Traffic Management in Mixed Traffic Road Networks: A Methodological Approach Integrating Game Theory, Bilevel Optimization, and C-ITS
Authors: Kotsi Areti , Politis Ioannis , Mitsakis Evangelos
Published: December 2024

The integration of Connected Vehicles into conventional traffic systems presents significant challenges due to the diverse behaviors and objectives of different drivers. Conventional vehicle drivers typically follow User Equilibrium principles, aiming to minimize their individual travel times without considering the overall network impact. In contrast, Connected Vehicle drivers, guided by real-time information from central authorities or private service providers, can adopt System Optimum strategies or Cournot-Nash oligopoly behaviors, respectively. The coexistence of these distinct player classes in mixed-traffic environments complicates the task of achieving optimal traffic flow and network performance. This paper presents a comprehensive framework for optimizing mixed-traffic road networks through a multiclass traffic assignment model. The framework integrates three distinct types of players: conventional vehicle drivers adhering to User Equilibrium principles, Connected Vehicle drivers following System Optimum principles under a central governing authority, and Connected Vehicle drivers operating under Cournot-Nash oligopoly conditions with access to services from private companies. The methodology includes defining a model to achieve optimal mixed equilibria, designing an algorithm for multiclass traffic assignment, formulating strategic games to analyze player interactions, and establishing key performance indicators to evaluate network efficiency and effectiveness. The framework is applied to a real-world road network, validating its practicality and effectiveness through computational results. The extraction and analysis of computational results are used to propose optimal traffic management policies for mixed-traffic environments. The findings provide significant insights into the dynamics of mixed traffic networks and offer practical recommendations for improving traffic management in increasingly complex urban transportation systems.
Risk Factors and Postoperative Outcomes in Pouchitis Following Restorative Proctocolectomy: An 18-Year Single-Center Study
Authors: Bertin Luisa , Nasrallah Mohamad , Redavid Carlo , Bonazzi Erica , Maniero Daria , Lorenzon Greta , De Barba Caterina , Facchin Sonia , Scarpa Marco , Ruffolo Cesare , Angriman Imerio , Buda Andrea , Fassan Matteo , Lacognata Carmelo , Barberio Brigida , Zingone Fabiana , Savarino Edoardo Vincenzo
Published: December 2024

Background/Objectives: Restorative proctocolectomy with ileo-anal pouch anastomosis (IPAA) remains the preferred surgical treatment for ulcerative colitis (UC). However, complications like pouchitis can occur. This study aimed to describe patients who underwent IPAA for inflammatory bowel disease (IBD) at Padua Hospital from 2005 to 2023 and identify risk factors for pouchitis. Secondary objectives included evaluating the effectiveness of biological therapy in chronic antibiotic-refractory pouchitis (CARP), Crohn’s disease of the pouch (CDP), and Crohn’s-like inflammation of the pouch (CDLPI), and assessing risk factors for pouch failure. Methods: This retrospective, observational study included 109 patients whose data were collected from medical records. Univariate logistic regression was used to analyze associations between preoperative and postoperative factors and outcomes such as acute pouchitis and pouch failure. The effectiveness of biological therapy was assessed by measuring changes in the Pouchitis Disease Activity Index (PDAI) and the Modified Pouchitis Disease Activity Index (mPDAI) over a 12-month treatment period. Results: Univariate logistic regression revealed significant associations between preoperative extraintestinal manifestations (OR 3.569, 95% CI 1.240–10.720), previous diagnosis of Crohn’s disease (OR 10.675, 95% CI 1.265–90.089), and transmural inflammation at cross-sectional imaging before surgery (OR 3.453, 95% CI 1.193–9.991) with an acute pouchitis risk. Pouch failure was significantly associated with a previous diagnosis of Crohn’s disease (OR 9.500, 95% CI 1.821–49.571) and post-surgical fistulas (OR 41.597, 95% CI 4.022–430.172). Biological therapy led to a significant reduction in the PDAI score in patients with CARP, decreasing from a median of 10 to 4 (p = 0.006). Similarly, in patients with CDP or CDLPI, the mPDAI score was significantly reduced from a median of 9 to 1 (p = 0.034), with remission achieved in 5/6 (83.3%) of these patients. Conclusions: This study provides valuable insights into the management of IPAA patients and highlights the importance of early identification and treatment of risk factors for pouchitis and failure. Biological therapy demonstrated significant effectiveness in reducing disease activity in patients with CARP, CDP, and CDLPI, suggesting its role as a crucial component in managing these complications.
Correction: Mallphanov et al. Novel Approach to Increasing the Amplitude of the Mechanical Oscillations of Self-Oscillating Gels: Introduction of Catalysts Both as Pendant Groups and as Crosslinkers. Gels 2024, 10, 727
Authors: Mallphanov Ilya L. , Eroshik Michail Y. , Safonov Dmitry A. , Sychev Alexander V. , Bulakov Vyacheslav E. , Lavrova Anastasia I.
Published: December 2024

In the original publication [...]
Genetic Variations on Chromosome 6p21 Are Associated with Asthma Risk and Disease Severity: A Case–Control Study from Pakistan
Authors: Aslam Aqsa , Vijverberg Susanne J. H. , Zee Anke-Hilse Maitland-van der , Sabar Muhammad Farooq
Published: December 2024

Background: Genetic factors play a role in asthma severity. However, low- and middle-income countries have minimal contribution to genomic asthma research. The current study investigates the influence of an important genetic asthma region (6p21) on severe asthma in a cohort of asthmatics in Pakistan. Materials and Methods: In this case–control study, mild to severe asthmatic patients (n = 255) and controls (n = 260) were enrolled from Lahore, Pakistan. Blood samples were collected, and genomic DNA was extracted for the genotyping of 11 single nucleotide polymorphisms located in the 6p21 region. Severe asthma was defined based on the defined daily dose of inhaled corticosteroids equivalent to 200 mcg of beclomethasone dipropionate (as per the global initiative for asthma guidelines). An additive genetic model was followed to find the associations between these variants and the outcome. Univariate and multivariate logistic regression, adjusted for confounders, was performed. Odds ratio (OR), 95% confidence interval (95% CI), p-value, and q-values after FDR adjustment were estimated. Results: The genetic variants rs3025028, rs987870, and rs3025039 showed strong associations with the incidence of asthma with odds ratios of 1.58, 1.62, and 2.70 (95% CI = 1.16–2.16, 1.15–2.30, and 1.40–5.39, respectively). Further stratification analysis to study the risk of severe asthma also revealed markedly significant associations for rs3025020 and rs1799964 (OR = 2.28 and 2.99; 95% CI = 1.39–3.86 and 1.75–5.33, respectively). However, the SNPs rs2070600, rs987870, and rs3025039 also showed a significant relationship with the severity (OR = 2.34, 1.75, and 2.72; 95% CI = 1.02–5.97, 1.07–2.98, and 1.11–7.71, respectively), but FDR-adjusted q-values were insignificant (0.10, 0.07, and 0.07, respectively). Conclusions: The 6p21 region variants rs3025028, rs987870, and rs3025039 are associated with the incidence, whereas rs3025020 and rs1799964 are associated with the risk of more severe asthma in the Pakistani population.
Conventional Cytogenetic Analysis of Solid Tumor Abnormalities: A 25-Year Review of Proficiency Test Results from the College of American Pathologists/American College of Medical Genetics and Genomics Cytogenetics Committee
Authors: Vanderschelden Rachel , Sukov William , Gardner Juli-Anne , Rehder Catherine , Levy Brynn , Velagaleti Gopalrao , Toydemir Reha , Tang Guilin , Boles Brittany , Cao Yang , Mixon Christopher , Zou Ying , Astbury Caroline , Tsuchiya Karen , Peterson Jess
Published: December 2024

Background: The joint College of American Pathologists/American College of Medical Genetics and Genomics Cytogenetics Committee works to ensure the competency and proficiency of clinical cytogenetic testing laboratories through proficiency testing (PT) programs for various clinical tests offered by such laboratories, including the evaluation of cytogenetic abnormalities in solid tumors. Methods: Review and analyze 25 years (1999–2023) of solid tumor chromosome analysis PT results, utilizing G-banded karyograms. A retrospective review of results from 1999 to 2023 was performed, identifying the challenges addressing solid tumors. The chromosomal abnormalities and overall performance were evaluated. Results: A total of 21 solid tumor challenges were administered during the period 1999–2018. No solid tumor challenges were administered during the period 2019–2023. Challenges consisted of metaphase images and accompanying clinical history for the evaluation of numerical and/or structural abnormalities. All 21 cases reached 80% grading consensus for abnormality recognition. However, five cases (24%) failed to reach consensus for nomenclature reporting by participating laboratories. These cases illustrate errors in reporting chromosomal abnormalities, including whole-arm translocations and those involving sex chromosomes. In addition, they highlight the challenges with differentiation of terminal and interstitial deletions, difficulties in identifying correct breakpoints, and omission of brackets in neoplastic cases. Conclusions: This comprehensive 25-year review demonstrates the exceptional proficiency of cytogenetic laboratories in accurately identifying chromosome abnormalities in solid tumors, while also highlighting the challenges of reporting specific types of chromosomal abnormalities.
Advancements and Challenges in Preimplantation Genetic Testing for Aneuploidies: In the Pathway to Non-Invasive Techniques
Authors: del Arco de la Paz Ana , Giménez-Rodríguez Carla , Selntigia Aikaterini , Meseguer Marcos , Galliano Daniela
Published: December 2024

The evolution of preimplantation genetic testing for aneuploidy (PGT-A) techniques has been crucial in assisted reproductive technologies (ARTs), improving embryo selection and increasing success rates in in vitro fertilization (IVF) treatments. Techniques ranging from fluorescence in situ hybridization (FISH) to next-generation sequencing (NGS) have relied on cellular material extraction through biopsies of blastomeres at the cleavage stage on day three or from trophectoderm (TE) cells of the blastocyst. However, this has raised concerns about its potential impact on embryo development. As a result, there has been growing interest in developing non-invasive techniques for detecting aneuploidies, such as the analysis of blastocoel fluid (BF), spent culture medium (SCM), and artificial intelligence (AI) models. Non-invasive methods represent a promising advancement in PGT-A, offering the ability to detect aneuploidies without compromising embryo viability. This article reviews the evolution and principles of PGT-A, analyzing both traditional techniques and emerging non-invasive approaches, while highlighting the advantages and challenges associated with these methodologies. Furthermore, it explores the transformative potential of these innovations, which could optimize genetic screening and significantly improve clinical outcomes in the field of assisted reproduction.
Genome-Wide Identification and Evolutionary Analysis of Functional BBM-like Genes in Plant Species
Authors: Hong Zhengyuan , Zhu Linghong , Liu Chaolei , Wang Kejian , Rao Yuchun , Lu Hongwei
Published: December 2024

Background/Objectives: BABY BOOM (BBM), a transcription factor from the APETALA2 (AP2) protein family, plays a critical role in somatic embryo induction and apomixis. BBM has now been widely applied to induce apomixis or enhance plant transformation and regeneration efficiency through overexpression or ectopic expression. However, the structural and functional evolutionary history of BBM genes in plants is still not well understood. Methods: The protein sequences of 10 selected plant species were used to locate the branch of BBM-Like by key domain identification and phylogenetic tree construction. The identified BBML genes were used for further conserved motif identification, gene structural analysis, miRNA binding site prediction, cis-acting element prediction, collinear analysis, protein–protein interaction network construction, three-dimensional structure modeling, molecular docking, and expression pattern analysis. Results: A total of 24 BBML proteins were identified from 10 representative plant species. Phylogenetic relationship analysis displayed that BBML proteins from eudicots and monocots were divided into two clusters, with monocots exhibiting a higher number of BBMLs. Gene duplication events indicated that whole genome/segmental duplication were the primary drivers of BBML genes’ evolution in the tested species, with purifying selection playing a key role during evolution processes. Comparative analysis of motif, domains, and gene structures revealed that most BBMLs were highly evolutionarily conserved. The expression patterns of BBML genes revealed significant tissue specificity, particularly in the root and embryo. We also constructed protein–protein interaction networks and molecular docking models to identify functional pathways and key amino acid residues of BBML proteins. The functions of BBMLs may differ between monocots and eudicots, as suggested by the functional enrichment of interacting proteins. Conclusions: Our research delved into the molecular mechanism, evolutionary relationships, functional differentiation, and expression patterns of BBML genes across plants, laying the groundwork for further investigations into the molecular properties and biological roles of BBMLs.
Is Critias a Sophist?
Authors: MacPhail Eric
Published: December 2024

The coherence and indeed the reality of the sophists as a philosophical school or movement has been contested and debated in modern scholarship, with inconclusive results. While their collective identity, not to mention their exemplarity, is subject to probing scrutiny, we usually have a fairly good idea of which historical figures we mean when we speak of the sophists. However, the case of Critias, the most infamous of the Thirty Tyrants of Athens, is particularly challenging since he does not seem to fit the professional profile of the other figures who are generally recognized as sophists and with whom his fragments have been edited and collected. This essay will briefly reconsider Critias’ candidacy as one of the ancient Greek sophists, not on the basis of what might be conjecturally reconstituted as his own philosophy, but rather on the basis of his association with the notion of the Greek or Sophistic Enlightenment. This notion and the periodization that it implies will be the focus of attention.
Healthcare Access and County-Level COVID-19 Mortality: Exploring the Impact of Hospital Proximity and Uninsurance Rates
Authors: Benavidez Gabriel A. , Zgodic Anja
Published: December 2024

Background/Objectives: Many social and environmental factors contribute to the disproportionate burden of COVID-19 mortality. Access to healthcare services has not been thoroughly examined as a factor contributing to COVID-19 mortality. This study examines distance to ERs and ICUs, uninsurance rates, and county-level COVID-19 mortality rates. Methods: Using data from the American Hospital Association survey, we identified hospitals providing emergency and intensive care services. Hospital locations were geocoded, and straight-line distance was calculated from the population-weighted county centroid. The county proportion of uninsured residents came from the American Community Survey. Generalized linear regression models with a log-link were used to examine study factors and county COVID-19 mortality rates. Results: A total of 2640 (84.0%) U.S. counties or county-equivalents were included in this analysis. The median COVID-19 mortality rate was 240 deaths per 100,000. In adjusted models, increasing distance to ERs (IRR: 0.95; 95% CI: 0.92, 0.98) or ICUs (IRR: 0.61; 95% CI: 0.57, 0.65) was not significantly associated with increased COVID-19 mortality. The proportion of residents (IRR: 3.81; CI: 2.58, 5.62) uninsured was significantly associated with increased COVID-19 mortality rates. Conclusions: Being in close proximity to hospital-based healthcare services may not provide any significant benefit for COVID-19 mortality outcomes, considering that hospitals are largely located in more densely populated areas conducive to COVID-19 spread. Financial barriers may largely contribute to persons avoiding necessary COVID-19 care. To continue to combat COVID-19 and future pandemics, greater attention should be focused on eliminating financial barriers to receiving medically necessary care.
Climate Risk and Conservation Challenges at Palermo’s Specola Museum
Authors: Carotenuto Maria Rosalia , Chinnici Ileana , Camuffo Dario , della Valle Antonio , Prestileo Fernanda , Megna Bartolomeo , Cavallaro Giuseppe , Lazzara Giuseppe
Published: December 2024

The Specola Museum is housed on the premises of the old Palermo Observatory, founded in 1790, and preserves most of the observatory’s cultural heritage. Environmental monitoring following the activation of air conditioning systems in 2018 revealed significant deviations from the historic thermo-hygrometric trends, with particularly dangerous fluctuations in relative humidity. A notable example of the impact of these changes is a 19th-century painted wooden Model of Mars, displayed in the Merz Hall since 2021. In less than two years, the Model has shown progressive damage to its paint layers. Conservation actions have been adopted to stop the deterioration process, but the risk of further deterioration phenomena involving other objects is expected to increase substantially in the absence of intervention. This paper presents the outcomes of a preliminary study on the thermo-hygrometric conditions in the Merz Hall. Based on the European Standard EN 15757: 2010 and the Italian Legislative decree of 10 May 2001, safe ranges for temperature and relative humidity have been identified for the long-term preservation of the collection. These findings will inform future climate management strategies in the museum.
Digital Immortality in Palaeoanthropology and Archaeology: The Rise of the Postmortem Avatar
Authors: Wilkinson Caroline M. , Roughley Mark A. , Shrimpton Sarah L.
Published: December 2024

It has been proposed that we are entering the age of postmortalism, where digital immortality is a credible option. The desire to overcome death has occupied humanity for centuries, and even though biological immortality is still impossible, recent technological advances have enabled possible eternal life in the metaverse. In palaeoanthropology and archaeology contexts, we are often driven by our preoccupation with visualising and interacting with ancient populations, with the production of facial depictions of people from the past enabling some interaction. New technologies and their implementation, such as the use of Artificial Intelligence (AI), are profoundly transforming the ways that images, videos, voices, and avatars of digital ancient humans are produced, manipulated, disseminated, and viewed. As facial depiction practitioners, postmortalism crosses challenging ethical territory around consent and representation. Should we create a postmortem avatar of someone from past just because it is technically possible, and what are the implications of this kind of forced immortality? This paper describes the history of the technologically mediated simulation of people, discussing the benefits and flaws of each technological iteration. Recent applications of 4D digital technology and AI to the fields of palaeoanthropological and historical facial depiction are discussed in relation to the technical, aesthetic, and ethical challenges associated with this phenomenon.
A Framework for Multi-Objective Optimization in Energy Retrofit of Heritage Museums: Enhancing Preservation, Comfort, and Conservation Conditions
Authors: Gea-Salim Camila , Flores-Larsen Silvana , Hongn Marcos , Gonzalez Silvina
Published: December 2024

Energy rehabilitation of heritage buildings used as museums presents unique challenges, as it must balance the preservation of cultural heritage with achieving comfortable indoor conditions for visitors and staff. These objectives often conflict, requiring innovative approaches. We propose a methodology that uses dynamical simulation with EnergyPlus and multi-objective optimization with jEPlus + EA software to reduce energy consumption and maximize comfort while maintaining architectural integrity and conservation conditions evaluated by a quantitative analysis based on the European Standards. The framework also includes monitoring microclimate conditions and assessing the risk levels of the exposed collections. A case study in Salta City, Argentina—a historical building repurposed as a museum—illustrates this approach. Results show improved energy performance and comfort conditions for visitors, with an annual energy requirement of about 36% of the original requirement (16.4 kWh/m2 versus 45 kWh/m2) and a decrease of about 32% in the discomfort hours (from 5362 h to 3643 h). Additionally, conservation conditions improved, achieving a more stable microclimate for air temperature and relative humidity. The framework proved useful for evaluating retrofit solutions that enhance the building’s energy performance and comfort without compromising the conservation conditions of the exhibited collections.
The Collaborative Redesign of ‘Authentic’ Technology-Enhanced Learning: Analysis of a Change Laboratory Research-Intervention in Engineering Higher Education
Authors: Moffitt Philip , Bligh Brett
Published: December 2024

This paper contributes to international debates about ‘authenticity’ in higher education, especially where this is embroiled in technology-oriented aspects of educational policy and practice. It describes activity theoretical research in a setting of engineering higher education, in industrial attachments taking place at distance, during which students and lecturers experience problems with technology-mediated interactions. Prior to the research-intervention that we describe in the paper, these interactions had been implemented through top-down policy, with work-related practices being conducted in the name of authenticity—a notion used in framing education as preparing students for economic work, in which digital technologies are increasingly embroiled. We describe an activity theoretical approach, and an online Change Laboratory methodology, through which students and lecturers envision and enact change to these practices. Their activity is thus reconfigured through their confrontation and renegotiation of authenticity. Our core contribution is to illustrate, through exposing and aggravating contradictions in technology-mediated activity, how practice in higher education can be considered authentic in and of itself, as distinct from solely having authenticity in preparing students for economic work.
Botanical Roots and Word Origins: A Systematic Reconstruction of Alor Plant Name Etymologies
Authors: Ong Brenda Man Qing , Perono Cacciafoco Francesco
Published: December 2024

This research provides a systematic reconstruction of nine botanical names from the Papuan languages spoken on Alor Island. Although genealogical links among the Papuan Timor–Alor–Pantar languages have been previously investigated, linguistic micro-studies of the hypothesized ‘shared’ ancestor languages within languages of the individual islands remain sparse. This study has three main aims: Firstly, to provide etymological reconstructions of Alor–Pantar Papuan words on a micro-level, focusing on Alor Island and specifically on plant names, which represent a cornerstone of Alor culture and history. Adopting the Comparative Method comparing cognates of 15 local languages, this research postulates historical phonetic shifts and language alignment phenomena and proposes proto-forms of the words in proto-Alor. Secondly, to shed light on possible prehistoric language contact and settlement patterns. A key finding suggests that the initial consonantal shifts *b > b > f > p in the languages is a probable loanword feature originating from the Austronesians. The geographical spread could indicate the influence of external trade and/or settlement patterns within the Papuan populations. Lastly, to provide a backbone for future etymological research on Papuan languages in Alor (and beyond) by mapping out aspects of language origins and phonetic influences and establish features of the shared proto-language(s).
Quality Evaluation and Shipping Export Potential of ‘Eliteggul’ Korean Melon (Cucumis melo var. makuwa) to Singapore Using MAP Technology
Authors: Jang Yu-Jin , Ko Da-Yeong , Ryu Jung-A , Kang Min-Gu , Han Jeung-Sul , Ku Kang-Mo
Published: December 2024

Korean melons (Cucumis melo var. makuwa), which have a short shelf life and are prone to browning, face challenges when exported over long-distance via maritime shipping. However, the high firmness and low suture browning rate of the ‘Eliteggul’ cultivar suggest its potential suitability for long-distance export. This study aimed to evaluate the export suitability of ‘Eliteggul’ by comparing it to the widely grown cultivar ‘Alchankkul’. Consumer preferences were assessed based on fragrance, sweetness, overall preference, appearance, and firmness. Both cultivars were grown and harvested in a commercial greenhouse in Seongju-gun, South Korea. After being treated with modified atmosphere film, the fruits were stored at 6 °C for 1 day, transported to Singapore in a shipping container at 7.4 °C for 13 days, and distributed at 22 °C for 3 days. After 14 days of storage, analysis in Singapore showed that ‘Eliteggul’ maintained a significantly higher firmness at 12.9 ± 0.3 kg·cm2 compared to ‘Alchankkul’, which showed firmness at 8.1 ± 0.4 kg·cm2. Additionally, ‘Eliteggul’ exhibited less browning on the white sutures at 1.3 ± 0.1, indicating better preservation of its quality than ‘Alchankkul’, at 2.0 ± 0.3. Even at 14 + 3 days, ‘Eliteggul’ maintained a firmness of 12.8 ± 0.2 kg·cm2, which was significantly higher than that of ‘Alchankkul’, which showed a firmness of 7.7 ± 0.5 kg·cm2. Consumer preference surveys (n = 78) conducted in the local market revealed that customers favored the fragrance and sweetness of ‘Alchankkul’. Correspondingly, an analysis of volatile organic compounds (VOCs) from melon juice using a Twister stir bar method showed that ‘Alchankkul’ had significantly higher levels of ethyl(methylthio)acetate, ethyl propanoate, and benzyl alcohol, known to contribute to a sweet flavor. Furthermore, a thermal desorption tube VOCs test indicated that hexyl acetate and 2-methylbutyl 2-methylbutyrate were also significantly higher in ‘Alchankkul’. Despite this result, there was no significant difference in the overall preference scores between the two cultivars. This study indicates that ‘Eliteggul’, with a superior firmness, reduced browning, and high consumer acceptance, is ideal for long-distance export, offering foundational data to bridge the research gap in export-oriented melons and support the broader distribution of Korean cultivars.