bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Drug reaction with eosinophilia and systemic symptoms (DRESS)-induced hemophagocytic disorder
Authors: Pope Eliza I. , Au Hosanna , Levy Deborah M. , Verstegen Ruud H. J.
Published: November 2022

Hemophagocytic disorders are severe and life-threatening conditions that can be genetic in origin [i.e., primary hemophagocytic lymphohistiocytosis (HLH)] or result from infections (i.e., secondary hemophagocytic lymphohistiocytosis), rheumatologic disease [i.e., macrophage activation syndrome (MAS)], and less frequently immunodeficiency or metabolic disease. Although rare, drug-induced hemophagocytosis needs to be considered in the work-up as it requires specific management strategies. Most drug-induced hemophagocytic disorders are related to Drug Reaction with Eosinophilia and Systemic Symptoms (DRESS). We present the case of a 7-year-old girl who initially presented with fever, maculopapular rash, and unilateral lymphadenopathy, who went on to develop hemophagocytosis secondary to DRESS caused by prolonged combination treatment with amoxicillin/clavulanic acid and trimethoprim/sulfamethoxazole. This case illustrates the importance of considering adverse drug reactions in the evaluations of patients with a hemophagocytic process.
Artificial intelligence-based internet hospital pharmacy services in China: Perspective based on a case study
Authors: Bu Fengjiao , Sun Hong , Li Ling , Tang Fengmin , Zhang Xiuwen , Yan Jingchao , Ye Zhengqiang , Huang Taomin
Published: November 2022

Background: Recently, internet hospitals have been emerging in China, saving patients time and money during the COVID-19 pandemic. In addition, pharmacy services that link doctors and patients are becoming essential in improving patient satisfaction. However, the existing internet hospital pharmacy service mode relies primarily on manual operations, making it cumbersome, inefficient, and high-risk.

Objective: To establish an internet hospital pharmacy service mode based on artificial intelligence (AI) and provide new insights into pharmacy services in internet hospitals during the COVID-19 pandemic.

Methods: An AI-based internet hospital pharmacy service mode was established. Initially, prescription rules were formulated and embedded into the internet hospital system to review the prescriptions using AI. Then, the “medicine pick-up code,” which is a Quick Response (QR) code that represents a specific offline self-pick-up order, was created. Patients or volunteers could pick up medications at an offline hospital or drugstore by scanning the QR code through the window and wait for the dispensing machine or pharmacist to dispense the drugs. Moreover, the medication consultation function was also operational.

Results: The established internet pharmacy service mode had four major functional segments: online drug catalog search, prescription preview by AI, drug dispensing and distribution, and AI-based medication consultation response. The qualified rate of AI preview was 83.65%. Among the 16.35% inappropriate prescriptions, 49% were accepted and modified by physicians proactively and 51.00% were passed after pharmacists intervened. The “offline self-pick-up” mode was preferred by 86% of the patients for collecting their medication in the internet hospital, which made the QR code to be fully applied. A total of 426 medication consultants were served, and 48.83% of them consulted outside working hours. The most frequently asked questions during consultations were about the internet hospital dispensing process, followed by disease diagnosis, and patient education. Therefore, an AI-based medication consultation was proposed to respond immediately when pharmacists were unavailable.

Conclusion: The established AI-based internet hospital pharmacy service mode could provide references for pharmacy departments during the COVID-19 pandemic. The significance of this study lies in ensuring safe/rational use of medicines and raising pharmacists’ working efficiency.
Osteopontin: An important protein in the formation of kidney stones
Authors: Jia Qingxia , Huang Ziye , Wang Guang , Sun Xia , Wu Yuyun , Yang Bowei , Yang Tongxin , Liu Jianhe , Li Pei , Li Jiongming
Published: November 2022

The incidence of kidney stones averages 10%, and the recurrence rate of kidney stones is approximately 10% at 1 year, 35% at 5 years, 50% at 10 years, and 75% at 20 years. However, there is currently a lack of good medicines for the prevention and treatment of kidney stones. Osteopontin (OPN) is an important protein in kidney stone formation, but its role is controversial, with some studies suggesting that it inhibits stone formation, while other studies suggest that it can promote stone formation. OPN is a highly phosphorylated protein, and with the deepening of research, there is growing evidence that it promotes stone formation, and the phosphorylated protein is believed to have adhesion effect, promote stone aggregation and nucleation. In addition, OPN is closely related to immune cell infiltration, such as OPN as a pro-inflammatory factor, which can activate mast cells (degranulate to release various inflammatory factors), macrophages (differentiated into M1 macrophages), and T cells (differentiated into T1 cells) etc., and these inflammatory cells play a role in kidney damage and stone formation. In short, OPN mainly exists in the phosphorylated form in kidney stones, plays an important role in the formation of stones, and may be an important target for drug therapy of kidney stones.
Do antibody–drug conjugates increase the risk of sepsis in cancer patients? A pharmacovigilance study
Authors: Xia Shuang , Zhao Yi-Chang , Guo Lin , Gong Hui , Wang Yi-Kun , Ma Rui , Zhang Bi-Kui , Sheng Yue , Sarangdhar Mayur , Noguchi Yoshihiro , Yan Miao
Published: November 2022

Introduction: Antibody–drug conjugates (ADCs) produce unparalleled efficacy in refractory neoplasms but can also lead to serious toxicities. Although ADC-related sepsis has been reported, the clinical features are not well characterized in real-world studies.

Objective: The aim of this study was to identify the association between ADCs and sepsis using FAERS data and uncover the clinical characteristics of ADC-related sepsis.

Methods: We performed disproportionality analysis using FAERS data and compared rates of sepsis in cancer patients receiving ADCs vs. other regimens. Associations between ADCs and sepsis were assessed using reporting odds ratios (RORs) and information component (IC). For each treatment group, we detected drug interaction signals, and conducted subgroup analyses (age, gender, and regimens) and sensitivity analyses.

Results: A total of 24,618 cases were reported with ADCs between Q1, 2004 and Q3, 2021. Sepsis, septic shock, multiple organ dysfunction syndrome, and other sepsis-related toxicities were significantly associated with ADCs than other drugs in this database. Sepsis and multiple organ dysfunction syndrome have the highest safety concerns with ADCs compared with other anticancer monotherapies. Gemtuzumab ozogamicin and inotuzumab ozogamicin showed increased safety risks than other ADCs. For the top nine ADC-related sepsis, males showed higher sepsis safety concern than females (p <0.001); however, age did not exert influence on the risk of sepsis. We identified that 973 of 2,441 (39.9%) cases had acute myeloid leukemia (AML), and 766 of 2613 (29.3%) cases on ADCs died during therapy. Time-to-onset analysis indicated ADC-related sepsis is prone to occur within a month after administration. Co-administration of ADCs with colony-stimulating factors, proton pump inhibitors, H2-receptor antagonists, or CYP3A4/5 inhibitors showed to synergistically increase the risk of sepsis-related toxicities.

Conclusion: Antibody–drug conjugates may increase the risk of sepsis in cancer patients, leading to high mortality. Further studies are warranted to characterize the underlying mechanisms and design preventive measures for ADC-related sepsis.
Assessment by ABPM verified the presence of hypertension in patients with self-reported hypertension, pregnant women, as well as differences between ethnicities in women aged 38-39 years in the Ribeirão Preto cohort
Authors: Brandão Tetzi Oliveira , Veiga Eduardo Carvalho de Arruda , Levy Rozeli Ferreira , Damaso Enio Luis , Sandrim Valeria Cristina , Cavalli Ricardo Carvalho
Published: November 2022

Introduction: Arterial hypertension is a global health problem and one of the main risk factors for cardiovascular diseases (CVD), and therefore for morbidity and mortality among adult men and women. Factors related to obstetric history, family history, sociodemographic characteristics, and lifestyle habits are known determinants of arterial hypertension.

Methods: Case-control study of women belonging to the 1978/79 birth cohort conducted in the city of Ribeirão Preto/SP. Sociodemographic data, presence of comorbidities, maternal comorbidities, paternal comorbidities, comorbidities during pregnancy, and biometric and biophysical markers associated with blood pressure measured by 24-h ambulatory blood pressure monitoring (ABPM) were assessed in women aged 38–39 years. We want to study which variables of the previous sentence are related to the presence of hypertension measured by ABPM.

Results: Data from 281 women were analyzed. Our results showed that ethnicity, a history of hypertension, and gestational hypertension reported by the women were significantly associated with the presence of hypertension measured by ABPM. Other factors such as marital status, educational level, comorbidities of the woman, paternal or maternal comorbidities, anthropometric measurements or serum levels of cardiovascular markers were not associated with the presence of hypertension measured by ABPM.

Conclusion: We conclude that ethnicity, self-reported hypertension, and gestational hypertension are associated with arterial hypertension measured by ABPM.
Reducing brain TACE activity improves neuroinflammation and cardiac function in heart failure rats
Authors: Yu Yang , Xue Baojian , Irfan Nafis Md , Beltz Terry , Weiss Robert M , Johnson Alan Kim , Felder Robert B , Wei Shun-Guang
Published: November 2022

Tumor necrosis factor (TNF)-α converting enzyme (TACE) is a key metalloprotease mediating ectodomain shedding of a variety of inflammatory mediators, substrates, and growth factors. We previously reported that TACE-mediated production of TNF-α in the hypothalamic paraventricular nucleus (PVN) contributes to sympathetic excitation in heart failure (HF). Here, we sought to determine whether central interventions in TACE activity attenuate neuroinflammation and improve cardiac function in heart failure. Myocardial infarction-induced HF or sham-operated (SHAM) rats were treated with bilateral paraventricular nucleus microinjection of a TACE siRNA or a 4-week intracerebroventricular (ICV) infusion of the TACE inhibitor TAPI-0. Compared with SHAM rats, scrambled siRNA-treated HF rats had higher TACE levels in the PVN along with increased mRNA levels of TNF-α, TNF-α receptor 1 and cyclooxygenase-2. The protein levels of TNF-α in cerebrospinal fluid and phosphorylated (p-) NF-κB p65 and extracellular signal-regulated protein kinase (ERK)1/2 in the PVN were also elevated in HF rats treated with scrambled siRNA. The expression of these inflammatory mediators and signaling molecules in the PVN of HF rats were significantly attenuated by TACE siRNA. Interestingly, the mRNA level of TNF-α receptor 2 in the PVN was increased in HF treated with TACE siRNA. Moreover, sympathetic excitation, left ventricular end-diastolic pressure, pulmonary congestion, and cardiac hypertrophy and fibrosis were reduced by PVN microinjection of TACE siRNA. A 4-week treatment with intracerebroventricular TAPI-0 had similar effects to ameliorate these variables in HF rats. These data indicate that interventions suppressing TACE activity in the brain mitigate neuroinflammation, sympathetic activation and cardiac dysfunction in HF rats.
Enhancement of cucumber resistance under salt stress by 2, 4-epibrassinolide lactones
Authors: He Xianxia , Wan Zilong , Jin Ning , Jin Li , Zhang Guobin , Lyu Jian , Liu Zeci , Luo Shilei , Yu Jihua
Published: November 2022

This study investigated the effects of exogenous 2, 4-epibrassinolide lactone (EBR) on the growth, photosynthetic pigments, antioxidant defense system, ion homeostasis, MAPK cascade and key genes of SOS signaling pathway of cucumber seedlings under salt stress using cucumber “Xinchun 4” as the test material. The experiment was set up with four treatments: foliar spraying of distilled water (CK), 50 mmol.L-1 NaCl (NaCl), 50 mmol.L-1 NaCl+foliar spray of 0.02 μmol.L-1 EBR (EBR+NaCl), and 50 mmol.L-1 NaCl+foliar spray of 24 μmol.L-1 Brassinazole (BRZ) (BRZ+NaCl). The results showed that EBR+NaCl treatment significantly increased plant height, above-ground fresh weight, total root length, total root surface area, average rhizome and photosynthetic pigment content compared to NaCl treatment. Meanwhile, compared with NaCl treatment, EBR+NaCl treatment significantly increased superoxide dismutase, catalase and ascorbate peroxidase (SOD, CAT and APX) activities, significantly promoted the accumulation of osmoregulatory substances (soluble sugars and proline), and thus effectively reduced malondialdehyde (MDA) content and relative electrical conductivity of cucumber leaves. Exogenous spraying of EBR also significantly reduced Na+/K+ under NaCl stress, effectively alleviating the toxic effects of Na+ ions. In addition, exogenous EBR induced the up-regulated expression of CsMAPK3, CsMAPK4, CsMAPK6 and CsMAPK9 genes in the MAPK cascade signaling pathway and CsSOS1, CsSOS2 and CsSOS3 genes in the SOS signaling pathway to enhance salt tolerance in cucumber under NaCl stress. Therefore, exogenous spraying EBR may effectively reduce the damage of salt stress on cucumber seedlings by improving antioxidant capacity, maintaining ion homeostasis and activating salt-tolerant related signaling pathways, which might promote the growth of cucumber seedlings and the establishment of root system morphology. This study provides a reference for EBR to improve the salt tolerance of cucumber.
Comparative phylogenomic analyses and co-expression gene network reveal insights in flowering time and aborted meiosis in woody bamboo, Bambusa oldhamii ‘Xia Zao’ ZSX
Authors: Zhao Wanqi , Guo Chunce , Yao Wenjing , Zhang Li , Ding Yulong , Yang Zhenzhen , Lin Shuyan
Published: November 2022

Woody bamboos have peculiar flowering characteristics with intervals ranging from several years to more than 100 years. Elucidating flowering time and reproductive development in bamboo could be beneficial for both humans and wildlife. To identity the mechanisms responsible for flowering time and embryo abortion in Bambusa oldhamii ‘Xia Zao’ ZSX, a transcriptome sequencing project was initiated to characterize the genes involved in developing flowers in this bamboo species. Morphological studies showed that pollen abortion in this bamboo species was mainly caused by a delay in tapetum degradation and abnormal meiotic process. Differential expression (DE) and optimized hierarchical clustering analyses identified three of nine gene expression clusters with decreasing expression at the meiosis of flowering stages. Together with enriched Gene Ontology Biological Process terms for meiosis, this suggests that their expression pattern may be associated with aborted meiosis in B. oldhamii ‘Xia Zao’. Moreover, our large-scale phylogenomic analyses comparing meiosis-related transcripts of B. oldhamii ‘Xia Zao’ with well annotated genes in 22 representative angiosperms and sequence evolution analyses reveal two core meiotic genes NO EXINE FORMATION 1 (NFE1) and PMS1 with nonsense mutations in their coding regions, likely providing another line of evidence supporting embryo abortion in B. oldhamii ‘Xia Zao’. Similar analyses, however, reveal conserved sequence evolution in flowering pathways such as LEAFY (LFY) and FLOWERING LOCUS T (FT). Seventeen orthogroups associated with flowering were identified by DE analyses between nonflowering and flowering culm buds. Six regulators found primarily in several connected network nodes of the photoperiod pathway were confirmed by mapping to the flowering time network in rice, such as Heading date (Hd3a) and Rice FT-like 1 (RFT1) which integrate upstream signaling into the downstream effectors. This suggests the existence of an intact photoperiod pathway is likely the key regulators that switch on/off flowering in B. oldhamii ‘Xia Zao’.
Increasing connections among temporal invariability, resistance and resilience of alpine grasslands on the Tibetan Plateau
Authors: Yang Yuting , Sun Yi , Niu Ben , Feng Yunfei , Han Fusong , Li Meng
Published: November 2022

Ecological stability contains multiple components, such as temporal invariability, resistance and resilience. Understanding the response of stability components to perturbations is beneficial for optimizing the management of biodiversity and ecosystem functioning. Although previous studies have investigated the effects of multiple perturbations on each stability component, few studies simultaneously measure the multiple stability components and their relationships. Alpine grasslands on the Tibetan Plateau are exposed to co-occurring perturbations, including climate change and human activities. Here, we quantified three stability components (temporal invariability, resistance, and resilience) of alpine grasslands on the Tibetan Plateau during periods of high (2000-2008) and low (2009-2017) human activity intensity, respectively. We focused on the effects of climate variables (temperature, precipitation, radiation) and human activities (grazing intensity) on covariation among stability components. The results show that (1) for periods of high and low human activity, temporal invariability was positively correlated with resistance and resilience, while resistance was independent of resilience; (2) the dimensionality of alpine grasslands decreased by almost 10%, from 0.61 in the first period to 0.55 in the second period, suggesting the increasing connections among temporal invariability, resistance and resilience of alpine grasslands; and (3) temperature but not grazing intensity dominated the changes in the dimensionality of stability. These findings improve our understanding of multi-dimensional stability and highlight the importance of climate variability on alpine grassland stability on the Tibetan Plateau.
Unlocking the hidden variation from wild repository for accelerating genetic gain in legumes
Authors: Singh Gurjeet , Gudi Santosh , Amandeep  , Upadhyay Priyanka , Shekhawat Pooja Kanwar , Nayak Gyanisha , Goyal Lakshay , Kumar Deepak , Kumar Pradeep , Kamboj Akashdeep , Thada Antra , Shekhar Shweta , Koli Ganesh Kumar , DP Meghana , Halladakeri Priyanka , Kaur Rajvir , Kumar Sumit , Saini Pawan , Singh Inderjit , Ayoubi Habiburahman
Published: November 2022

The fluctuating climates, rising human population, and deteriorating arable lands necessitate sustainable crops to fulfil global food requirements. In the countryside, legumes with intriguing but enigmatic nitrogen-fixing abilities and thriving in harsh climatic conditions promise future food security. However, breaking the yield plateau and achieving higher genetic gain are the unsolved problems of legume improvement. Present study gives emphasis on 15 important legume crops, i.e., chickpea, pigeonpea, soybean, groundnut, lentil, common bean, faba bean, cowpea, lupin, pea, green gram, back gram, horse gram, moth bean, rice bean, and some forage legumes. We have given an overview of the world and India’s area, production, and productivity trends for all legume crops from 1961 to 2020. Our review article investigates the importance of gene pools and wild relatives in broadening the genetic base of legumes through pre-breeding and alien gene introgression. We have also discussed the importance of integrating genomics, phenomics, speed breeding, genetic engineering and genome editing tools in legume improvement programmes. Overall, legume breeding may undergo a paradigm shift once genomics and conventional breeding are integrated in the near future.
Straw incorporation and nitrogen reduction effect on the uptake and use efficiency of nitrogen as well as soil CO2 emission of relay strip intercropped soybean
Authors: Zheng Benchuan , Chen Ping , Du Qing , Yang Huan , Luo Kai , Wang Xiaochun , Yang Feng , Yong Taiwen , Yang Wenyu
Published: November 2022

Intercropping can increase crop N uptake and reduce carbon emissions. However, the effects of straw incorporation and N reduction on N use and carbon emissions in intercropping are still unclear. We explored the mechanism of N uptake, N use efficiency, and CO2 emissions in the wheat-maize-soybean relay strip intercropping system. A two-year field experiment was conducted with two straw managements, i.e., wheat straw incorporation (SI) and straw removal (SR), and four N application levels of soybean, i.e., 60 (N60), 30 (N30), 15 (N15), and 0 kg N ha-1 (N0). We assessed soil properties, CO2 emissions, and characteristics of roots, nodules, and aboveground N uptake of intercropped soybean. Results showed that geometry mean diameter of aggregate, soil porosity, soil total N, and soil urease activity were notably greater in SI than in SR. N input reduced from N60 to N30 did not significantly affect the soil total N content and urease activity in SI. The root length, root surface area, root volume, root biomass, root bleeding intensity, and inorganic N content of bleeding sap were greater in SI than in SR. In the SI, although the root length and surface area peaked at N60, the root biomass and inorganic N content of bleeding sap were insignificant between N60 and N30. The nodule number, nodule dry weight, nodule nitrogenase activity, and nodule nitrogen fixation potential in SI were notably increased compared with SR. The nodule nitrogen fixation potential in SI notably increased with the decrease of N input at the R3 stage, but it peaked in N30 at the R5 stage. On average, the aboveground N uptake and nitrogen recovery efficiency (RE) was notably higher by 43.7% and 76.8% in SI than in SR. SI+N30 achieved the greatest aboveground N uptake and RE. The CO2 emission and accumulated CO2 emission were notably greater in SI than in SR, and the accumulated CO2 emission of SI was the lowest with N30 input. In conclusion, SI+N30 promoted N uptake and utilization efficiency with reduced CO2 emissions during the soybean cropping season. It provides a potential strategy for sustainable agricultural development in intercropping systems.
Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance
Authors: Yang Chaochen , Wu Pengfei , Cao Yongqing , Yang Bingbing , Liu Linxiu , Chen Juanjuan , Zhuo Renying , Yao Xiaohua
Published: November 2022

The outbreak of anthracnose caused by Colletotrichum spp. represents a devastating epidemic that severely affects oil tea (Camellia oleifera) production in China. However, the unknown resistance mechanism to anthracnose in C. oleifera has impeded the progress of breeding disease-resistant varieties. In this study, we investigated the physiological responses of resistant and susceptible lines during C. gloeosporioides infection. Our results showed that the accumulation of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in both disease-resistant and susceptible lines increased by C. gloeosporioides infection. Also, disease-resistant lines exhibited lower MDA, but higher POD, SOD, and CAT activities compared to susceptible lines. The accumulation of flavonoids in both resistant and susceptible C. oleifera leaves increased following C. gloeosporioides infection, and the increase was greater in resistant lines. Further, we identified and functionally characterized the dihydroflavonol 4-reductase (CoDFR) from the resistant C. oleifera line. We showed that the full-length coding sequence (CDS) of CoDFR is 1044 bp encoding 347 amino acids. The overexpression of CoDFR in tobacco altered the expression of flavonoid biosynthetic genes, resulting in an increased flavonoid content in leaves. CoDFR transgenic tobacco plants exhibited increased anthracnose resistance. Furthermore, the transgenic plants had higher salicylic acid content. These findings offer potential insights into the pivotal role of CoDFR involved in flavonoid-mediated defense mechanisms during anthracnose invasion in resistant C. oleifera.
Cleaner production technologies for the amelioration of soil health, biomass and secondary metabolites in Ocimum basilicum L. under Indian Western Himalaya
Authors: Raj Yog , Ali Nilofer , Pati Aparna Maitra , Kumar Rakesh
Published: November 2022

Ocimum basilicum L. and its derived products are primarily consumed by humans; hence, agrochemical use seems inappropriate for its cultivation. However, farmers are accustomed to using rampant inorganic fertilizers to augment crop productivity, which has unintendedly engendered severe environmental perturbations. Concomitantly, farmers will soon have to confront the challenges of growing crops under suboptimal conditions driven by global climate change. Consequently, to develop a clean, sustainable, and resilient production technology, field experiments spanning over two years (2020 and 2021) were conducted, comprising three biostimulants, viz., vermicompost (0, 4, and 8 Mg ha−1), biofertilizer (uninoculated and inoculated), and liquid seaweed extract (without and at 7 ml L−1) in the Indian western Himalaya for the first time. Soil health indicators, leaf photosynthetic pigments, gaseous exchange, mineral contents, essential oil (EO) quantity, and composition were evaluated. Soil microbial respiration (SMR), microbial biomass carbon (MBC), organic carbon (OC), dehydrogenase (DHA), alkaline phosphatase (ALP), and β-glucosidase activities were increased by 36.23, 83.98, 30.61, 42.69, 34.00, and 40.57%, respectively, when compared with the initial soil status. The net photosynthetic rate (Pn) was significantly increased with the highest (8 Mg ha−1) and moderate (4 Mg ha−1) vermicompost dosages by 13.96% and 4.56%, respectively, as compared with the unfertilized control (0 Mg ha−1). Likewise, the biofertilizer and seaweed extract also enhanced Pn by 15.09% and 10.09%, respectively. The crop’s key EO constituents, viz., methyl chavicol and linalool, were significantly improved with the highest and moderate vermicompost rates of 2.71, 9.85%, and 1.18, 5.03%, respectively. Similarly, biofertilization and seaweed application also boosted methyl chavicol and linalool by 3.29, 8.67%, and 1.93, 3.66%, respectively. In both years, significantly higher herbage (8.86 and 11.25 Mg ha−1) and EO yield (113.78 and 154.87 kg ha−1) were recorded with a congregate treatment of the highest vermicompost dose, biofertilizer, and liquid seaweed extract. In conclusion, the integrated use of biostimulants having complementary properties can sustainably maximize the quantity and quality of O. basilicum and concomitantly ameliorate soil health. This study can inspire scientific communities and industries to develop second-generation biostimulant products, delivering better sustainability and resilience for a renaissance in agriculture.
Characterization and selection of endophytic actinobacteria for growth and disease management of Tea (Camellia sinensis L.)
Authors: Hazarika Shabiha Nudrat , Saikia Kangkon , Thakur Debajit
Published: November 2022

Endophytic microbes are vital for nutrient solubilization and uptake, growth, and survival of plants. Here, 88 endophytic actinobacteria (EnA) associated with five tea clones were isolated, assessed for their diversity, plant growth promoting (PGP), and biocontrol traits, and then used as an inoculant for PGP and disease control in host and non-host plants. Polyphasic methods, including phenotypic and genotypic characteristics led to their identification as Streptomyces, Microbacterium, Curtobacterium, Janibacter, Rhodococcus, Nocardia, Gordonia, Nocardiopsis, and Kribbella. Out of 88 isolates, 35 (39.77%) showed antagonistic activity in vitro against major fungal pathogens, viz. Fusarium oxysporum, Rhizoctonia solani, Exobasidium vexans, Poria hypobrunnea, Phellinus lamaensis, and Nigrospora sphaerica. Regarding PGP activities, the percentage of isolates that produced indole acetic acid, siderophore, and ammonia, as well as P-solubilisation and nitrogen fixation, were 67.05, 75, 80.68, 27.27, 57.95, respectively. A total of 51 and 42 isolates showed chitinase and 1-aminocyclopropane-1-carboxylic acid deaminase activity, respectively. Further, two potent Streptomyces strains KA12 and MA34, selected based on the bonitur scale, were screened for biofilm formation ability and tested in vivo under nursery conditions. Confocal laser scanning microscopy and the crystal violet staining technique revealed that these Streptomyces strains can form biofilms, indicating the potential for plant colonization. In the nursery experiment, they significantly enhanced the shoot and root biomass, shoot and root length, and leaf number in host tea plants. Additionally, treatment of tomato seeds by KA12 suppressed the growth of fungal pathogen Fusarium oxysporum, increased seed germination, and improved root architecture, demonstrating its ability to be used as a seed biopriming agent. Our results confirm the potential of tea endophytic actinobacterial strains with multifarious beneficial traits to enhance plant growth and suppress fungal pathogens, which may be used as bioinoculant for sustainable agriculture.
Biostimulation can prime elicitor induced resistance of grapevine leaves to downy mildew
Authors: Jacquens Lucile , Trouvelot Sophie , Lemaitre-Guillier Christelle , Krzyzaniak Yuko , Clément Gilles , Citerne Sylvie , Mouille Grégory , Moreau Estelle , Héloir Marie-Claire , Adrian Marielle
Published: November 2022

Using plant defense elicitors to protect crops against diseases is an attractive strategy to reduce chemical pesticide use. However, development of elicitors remains limited because of variable effectiveness in the field. In contrast to fungicides that directly target pathogens, elicitors activate plant immunity, which depends on plant physiological status. Other products, the biostimulants, can improve certain functions of plants. In this study, the objective was to determine whether a biostimulantviaeffects on grapevine physiology could increase effectiveness of a defense elicitor. A new methodology was developed to study biostimulant activity under controlled conditions usingin vitroplantlets. Both biostimulant and defense elicitor used in the study were plant extracts. When added to the culture medium, the biostimulant accelerated the beginning of plantlet growth and affected the shoot and root development. It also modified metabolomes and phytohormone contents of leaves, stems, and roots. When applied on shoots, the defense elicitor changed metabolite and phytohormone contents, but effects were different depending on whether plantlets were biostimulated or controls. Defense responses and protection againstPlasmopara viticola(downy mildew agent) were induced only for plantlets previously treated with the biostimulant, Therefore, the biostimulant may act by priming the defense elicitor action. In this study, a new method to screen biostimulants active on grapevine vegetative growth was used to demonstrate that a biostimulant can optimize the efficiency of a plant defense elicitor.
An empirical study on the moderating effect of environmental audit on the impact of economic development and ecological environment
Authors: Xiong Mingliang
Published: November 2022

Environmental audit is a new thing. As an important part of national audit, it is an important environmental governance supervision and control mechanism in China, but what is the macro environmental governance effect of environmental audit? Can environmental audit promote regional pollution control? Does environmental audit play a role in regulating the effect of environmental pollution control on the basis of economic development? The research on these issues has great practical and theoretical significance. Through questionnaire, 217 valid data were finally obtained as research samples. Using correlation analysis, partial correlation analysis, full regression, and stepwise regression analysis, on the basis of testing the explanatory power of economic development and environmental audit on ecological environment quality, the paper further tested the impact of the interactive multiplier of environmental audit and economic development on ecological environment quality. The study found that: not only do economic development and environmental auditing each have a significant positive correlation on the quality of the ecological environment; moreover, economic development and environmental auditing interact with each other to influence the ecological environment, and the degree of correlation and influence of environmental auditing on the ecological environment is greater than the degree of correlation and influence of economic development on the ecological environment. Environmental auditing has a significant positive moderating effect on economic development in the process of improving ecological and environmental quality, and on the basis of economic development, environmental auditing by the government has a more significant effect on the improvement of ecological and environmental quality. Studying this problem is not only conducive to guiding the government audit department to take on the role in the ecological environment governance, but also to more effectively control the environmental pollution problem, realizing the cooperation and mutual penetration of the role of government environmental audit in economic development and ecological environment protection policies. It provides reference for future empirical research using micro survey data, and also provides policy suggestions for economic construction, environmental audit, and ecological environment governance in various regions of China, and provides empirical evidence support for contingency theory.
Event-related potential and lexical decision task in dyslexic adults: Lexical and lateralization effects
Authors: Silva Patrícia Botelho , Oliveira Darlene Godoy , Cardoso Amanda Douat , Laurence Paulo Guirro , Boggio Paulo Sérgio , Macedo Elizeu Coutinho
Published: November 2022

Developmental dyslexia is a specific learning disorder that presents cognitive and neurobiological impairments related to different patterns of brain activation throughout development, continuing in adulthood. Lexical decision tasks, together with electroencephalography (EEG) measures that have great temporal precision, allow the capture of cognitive processes during the task, and can assist in the understanding of altered brain activation processes in adult dyslexics. High-density EEG allows the use of temporal analyses through event-related potentials (ERPs). The aim of this study was to compare and measure the pattern of ERPs in adults with developmental dyslexia and good readers, and to characterize and compare reading patterns between groups. Twenty university adults diagnosed with developmental dyslexia and 23 healthy adult readers paired with dyslexics participated in the study. The groups were assessed in tests of intelligence, phonological awareness, reading, and writing, as well as through the lexical decision test (LDT). During LDT, ERPs were recorded using a 128-channel EEG device. The ERPs P100 occipital, N170 occipito-temporal, N400 centro-parietal, and LPC centro-parietal were analyzed. The results showed a different cognitive profile between the groups in the reading, phonological awareness, and writing tests but not in the intelligence test. In addition, the brain activation pattern of the ERPs was different between the groups in terms of hemispheric lateralization, with higher amplitude of N170 in the dyslexia group in the right hemisphere and opposite pattern in the control group and specificities in relation to the items of the LDT, as the N400 were more negative in the Dyslexia group for words, while in the control group, this ERP was more pronounced in the pseudowords. These results are important for understanding different brain patterns in developmental dyslexia and can better guide future interventions according to the changes found in the profile.
Exposure to unpredictability and mental health: Validation of the brief version of the Questionnaire of Unpredictability in Childhood (QUIC-5) in English and Spanish
Authors: Lindert Natasha G. , Maxwell Megan Y. , Liu Sabrina R. , Stern Hal S. , Baram Tallie Z. , Poggi Davis Elysia , Risbrough Victoria B. , Baker Dewleen G. , Nievergelt Caroline M. , Glynn Laura M.
Published: November 2022

Unpredictability is increasingly recognized as a primary dimension of early life adversity affecting lifespan mental health trajectories; screening for these experiences is therefore vital. The Questionnaire of Unpredictability in Childhood (QUIC) is a 38-item tool that measures unpredictability in childhood in social, emotional and physical domains. The available evidence indicates that exposure to unpredictable experiences measured with the QUIC predicts internalizing symptoms including depression and anxiety. The purpose of the present study was to validate English and Spanish brief versions (QUIC-5) suitable for administration in time-limited settings (e.g., clinical care settings, large-scale epidemiological studies). Five representative items were identified from the QUIC and their psychometric properties examined. The predictive validity of the QUIC-5 was then compared to the QUIC by examining mental health in four cohorts: (1) English-speaking adult women assessed at 6-months postpartum (N = 116), (2) English-speaking male veterans (N = 95), (3) English-speaking male and female adolescents (N = 155), and (4) Spanish-speaking male and female adults (N = 285). The QUIC-5 demonstrated substantial variance in distributions in each of the cohorts and is correlated on average 0.84 (r’s = 0.81–0.87) with the full 38-item version. Furthermore, the QUIC-5 predicted internalizing symptoms (anxiety and depression) in all cohorts with similar effect sizes (r’s = 0.16–0.39; all p’s < 0.05) to the full versions (r’s = 0.19–0.42; all p’s < 0.05). In sum, the QUIC-5 exhibits good psychometric properties and is a valid alternative to the full QUIC. These findings support the future use of the QUIC-5 in clinical and research settings as a concise way to measure unpredictability, identify risk of psychopathology, and intervene accordingly.
E-commerce adoption among micro agri-business enterprise in Longsheng, China: The moderating role of entrepreneurial orientation
Authors: Li Hanfang , Liow Guateng , Yuan Shengjun
Published: November 2022

E-commerce in agri-business enterprises is a potent force in eradicating rural poverty in China. However, it has not reached its potential as many of the agri-business enterprises in rural areas are slow in re-thinking their distribution channel and implementing e-commerce. This study utilizes the multidimensionality of the Tornatsky and Fleisher Technology-Organization-Environment (TOE) framework to provide insights into why rural agri-business enterprises are not adopting e-commerce strategies. Specifically, it examines the moderating role of entrepreneurial orientation (EO) on the relationship between the sub-variables in the three contexts and e-commerce adoption. Empirical data from 192 micro agri-business enterprises were analyzed using PLS-SEM. The results show that relative advantage, organization readiness, competitive pressure, and government support have a direct positive impact on e-commerce adoption except for cost. The results also show that EO does not have a significant role in moderating the variables in the organizational context and environmental context. EO only moderated the relationship between relative advantage in the technology context and e-commerce adoption. This study advances research on e-commerce adoption by highlighting the importance of the owners' EO as a moderator between TOE factors and e-commerce adoption. It suggests that entrepreneurship must be pursued vigorously among agribusiness owners in rural China to enhance the adoption of e-commerce.
Study on influence factors of public participation willingness in substation project based on integrated TPB-NAM model
Authors: Ma Xin , Li Junpeng , Guo Fuli , Cui Caocao , Chen Tengfei , Xv Fan , Wang Wenbin
Published: November 2022

Public infrastructure, such as substations, is crucial for the advancement of the economy and society. However, the “not in my backyard” phenomenon is causing concern among the population, and these two things are at odds with one another. This study aims to investigate the driving mechanism that influences participation willingness of the public in order to promote the construction of substations, so the study proposes an integration model based on the planned behavior theory and the normative activation theory. Moreover, a structural equation model is created using the two dimensions, namely, social altruism and personal egoism, while data of 568 questionnaires are used for empirical research in combination with the “Decision-Making Trial and Evaluation Laboratory” method; these data are collected in the surrounding areas of three 110kV substations in Jiaozuo city, China. The key factors that affect participation willingness of the public are discussed, and the study demonstrates that the model is most significantly impacted by public trust, which is an a priori variable. Furthermore, the direct path coefficient of personal norms on participation willingness is the largest, which confirms that increased moral responsibility has a beneficial effect on project execution, and subjective norms contribute to the improvement of the assessment model overall since they are the main variables with the largest centrality degree in the system. The findings of this research better our understandings about the mechanism of “not in my backyard” and offer practical implications for its dissolution. On the basis of this, we present pertinent policy proposals for the “not in my backyard” effect that develops during the construction of public infrastructure.
Perceived stigma, discrimination and psychological problems among patients with epilepsy
Authors: Malik Najma Iqbal , Fatima Rabia , Ullah Irfan , Atta Mohsin , Awan Ashraf , Nashwan Abdulqadir J. , Ahmed Saeed
Published: November 2022

Objective

The current study sought to investigate the effects of perceived stigma and discrimination on epilepsy patients' psychological problems, such as depression, anxiety, and quality of life.

Study design

A purposive sampling technique was used in this cross-sectional study.

Materials and methods

The sample consisted of 186 patients with epilepsy. The Stigma Scale, Depression Anxiety Stress Scale, and Quality of life in Epilepsy-10 were used to measure the study variables.

Results

Findings showed that both the perception of stigma and discrimination have a significant positive correlation with depression as well as anxiety. Stigma is a significant positive predictor of depression and anxiety at [F (1, 184) = 27.8, 15.92,p< 0.001, 0.001, respectively] and explains 12.7 and 7.5% variance that could be attributed to Stigma. Stigma is also a significant predictor of quality of life at [F (1, 184) = 16.10,p< 0.001] and explains the 7.5% variance that could be attributed to Stigma. Results also indicate that discrimination is a significant positive predictor of depression and anxiety at [F (1, 184) = 32.39, 19.91,p< 0.001] and explains 15 and 9.8 % variance, respectively, that could be attributed to Stigma. However, stigma negatively predicts quality of life at [F (1, 184) = 20.34,p< 0.001] and explains 10 % variance. Non-significant differences were found in all the demographic variables (i.e., gender, socio-economic status, and disease duration), except the quality of life was significantly high among individuals with high Socio-economic status.

Conclusions

Stigma is significantly higher in epileptic patients and has a detrimental effect on the patient's quality of life, recovery, and prognosis. Thus, there is undoubtedly a need to address psychological issues, most notably the stigma associated with illnesses. Psychologists, psychiatrists, other physicians, and care givers of epileptic patients must pay close attention to the stigma in this patient population.
Relationship between allergic diseases and mental disorders in women: A systematic review and meta-analysis
Authors: Liu Lisha , Luo Chao , Zhang Mengni , Ao Xudong , Liu Huixia , Peng Shunlin
Published: November 2022

Background

The relationship between allergic diseases (AD) and mental disorders (MD) in women has not been fully systematically evaluated. We aimed at validating this correlation.

Methods

The relevant cohort and case-control studies from the establishment of the database to February 18, 2022 in PubMed, Embase, and Cochrane library were searched by computer. The researchers conducted the quality evaluation of the included articles by reviewing and discussing with reference to relevant standards, and conducted the analysis of the correlation between female patients with AD and MD by using Review Manager 5.4.

Results

Six observational studies from 2631 studies (n = 1160858 women) were assessed as medium and high-quality studies. The meta-analysis demonstrated that AD was correlated with MD in female patients (OR = 1.21, 95%CI: 1.14–1.29), including asthma (OR = 1.16, 95%CI: 1.11–1.22), allergic rhinitis (OR = 1.31, 95%CI: 1.06–1.63), and atopic dermatitis in women (OR = 1.37, 95%CI: 1.24–1.50) were associated with MD. At the same time, subgroup analysis was performed according to region, study design, criteria of AD and MD, and the results demonstrated that both AD and MD were correlated in these different conditions.

Conclusion

Allergic diseases in female patients do have an association with mental disorders.

Systematic review registration

[https://www.crd.york.ac.uk/PROSPERO/], identifier [CRD42022311146].