bluuebunny/crossref_metadata_2025 huggingface dataset:
Percutaneous Coronary Intervention Using the DynamX Sirolimus‐Eluting Bioadaptor: 12‐Month Clinical and Imaging Outcomes
Authors: Webster Mark , Scott Douglas , Menon Madhav , McClean Dougal , El-Jack Seif , Wilkins Gerard , Harding Scott A.
Published: October 2024

The DynamX Bioadaptor is a novel implant with uncaging elements that disengage after polymer resorption and permits restoration of vessel function, thereby potentially reducing late adverse cardiac events. We evaluated the safety and efficacy of this novel implant with imaging follow‐up at 6‐months, the time point when the bioadaptor starts to uncage. This prospective study enrolled 44 patients with up to two de novo lesions at seven centers in New Zealand treated with the DynamX Bioadaptor. Follow‐up was planned out to 12‐months. The primary safety and efficacy endpoints were 6‐month target lesion failure (TLF) and late lumen loss. Patients presented with non‐ST elevation myocardial infarction in 49%. Lesions (n = 45) were 16.0 ± 6.7 mm long, and 2.81 ± 0.41 mm in diameter. Device and procedure success were 100%, and acute lumen gain was 1.93 ± 0.35 mm. At 6‐months, median in‐device late lumen loss was 0.09 mm (IQR:0.05–0.16). Measured by intravascular ultrasound, the vessel, lumen, and device area remained constant. By optical coherence tomography, 96.4% ± 5.9% of struts per lesion were covered. Out to 12‐months, no TLF and no definite or probable device thrombosis were reported. In conclusion, this study provides evidence of the safety and performance of the DynamX Bioadaptor with excellent 12‐month clinical and 6‐month imaging outcomes comparable to best‐in‐class drug‐eluting stents (DES).

Trial Registration: ClinicalTrials.gov identifier: NCT03634020, NCT05464147, NCT04562805, and NCT04192747.
Supplementary Tutorial from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Tutorial for using the portal
Supplementary Table S2 from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Major data types on the portal
Supplementary Notes from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Supplementary Notes
Supplementary Figure S8 from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Exporting data for haplotype analysis
Supplementary Figure S7 from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

User interface for genetic association analysis on the portal
Supplementary Figure S5 from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Scatter plot and complete regression results measuring the association between mental health, amputation, and age
Supplementary Figure S3 from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Association between ARID5B variants and ALL in African and European ancestries
Supplementary Figure S1 from St. Jude Survivorship Portal: Sharing and Analyzing Large Clinical and Genomic Datasets from Pediatric Cancer Survivors
Authors: Matt Gavriel Y. , Sioson Edgar , Shelton Kyla , Wang Jian , Lu Congyu , Zaldivar Peraza Airen , Gangwani Karishma , Paul Robin , Reilly Colleen , Acić Aleksandar , Liu Qi , Sandor Stephanie R. , McLeod Clay , Patel Jaimin , Wang Fan , Im Cindy , Wang Zhaoming , Sapkota Yadav , Wilson Carmen L. , Bhakta Nickhill , Ness Kirsten K. , Armstrong Gregory T. , Hudson Melissa M. , Robison Leslie L. , Zhang Jinghui , Yasui Yutaka , Zhou Xin
Published: October 2024

Design and architecture of the St. Jude Survivorship Portal
Supplementary Table S8 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

Significant GSEA full list of Bulk RNA-seq for BT549 shTDO2-98 (IDO1 comp) vs. shTDO2-82
Supplementary Table S2 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

qPCR primers used in this study
Supplementary Table S10 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

Gene co-expression with TDO2 list of single-cell RNA-seq for MDA-MB-453 treated with Vehicle
Supplementary Table S1 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

shRNA used in this study.
Supplementary Figure S9 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

The AhR ChIP seq dataset shows high enrichments at the promoter region of ZEB1 transcript.
Supplementary Figure S7 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

AT-0174 reduced invasion in TDO2 OE cells.
Supplementary Figure S3 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

Tryptophan catabolism to kynurenine was enhanced in cells surviving in the anchorage independent condition over time.
Supplementary Figure S2 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

Increased tryptophan catabolism and accumulation of kynurenine and other downstream catabolites.
Supplementary Figure S11 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

AhR, ZEB1 and TDO2 expression in clinical breast cancer database.
Supplementary Figure S10 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Authors: Kuo Li-Wei , Crump Lyndsey S. , O’Neill Kathleen , Williams Michelle M. , Christenson Jessica L. , Spoelstra Nicole S. , Roy Micaela Kalani , Argabright Amy , Reisz Julie A. , D’Alessandro Angelo , Boorgula Meher P. , Goodspeed Andrew , Bickerdike Mike , Bitler Benjamin G. , Richer Jennifer K.
Published: October 2024

Genetic knockdown or pharmacological inhibition of TDO2 or AhR decreased ZEB1 mRNA expression but increased while IDO1 compensated; KYN treatment increased ZEB1 expression.
The Role of DLK1 Deficiency in Central Precocious Puberty and Association with Metabolic Dysregulation
Authors: d'Aniello Francesco , Mariniello Katia , Al Sayed Yasmin , Bhavsar Karishma , Read Jordan E. , Guasti Leonardo , Howard Sasha R.
Published: October 2024

Introduction: Precocious puberty is defined as the appearance of secondary sexual characteristics before the age of 8 years in girls and 9 years in boys. Central precocious puberty (CPP) is a rare condition that is diagnosed when premature activation of the hypothalamic-pituitary-gonadal axis is detected, in association with precocious breast development or testicular growth. Idiopathic CPP is historically considered to be the most common form, but in recent years defects in a small but growing number of genes regulating the timing of puberty have been identified in an increasing proportion of cases of CPP. Delta-like non-canonical Notch ligand 1 (DLK1) is understood to be one of the key genes involved in the etiology of CPP, although its mechanistic role is not yet fully understood. Case Presentation: We identified a novel de novo variant of DLK1 (c.835C>T; p.Gln279*) in an 8-year-old girl of Bangladeshi origin. She presented with an advanced Tanner staging of B4P4A2, significantly advanced bone age (BA, 13 years), a near-adult proportioned uterus, with a history of menarche at the age of 7.4 years. Diagnosis was confirmed by raised basal luteinizing hormone concentration. She was found to have truncal obesity associated with abnormal fasting insulin levels and mildly elevated cholesterol levels. These findings are consistent with previous literature describing an association between patients with DLK1 deficiency and an impaired metabolic profile. The patient was treated for 2 years with GnRH agonists with ongoing biochemical follow-up into adolescence. Conclusion: This case illustrates the susceptibility to metabolic derangement for patients with mutations in DLK1 and the need for ongoing monitoring after puberty. Our summary of previously identified DLK1 variants and their metabolic consequences demonstrates the frequency of obesity, lipid abnormalities, and insulin dysregulation in this patient cohort in childhood and beyond. This knowledge can guide future clinical practice for patients with CPP patients due to DLK1 deficiency.
Crítica fora do esquadro: homenagem a Antonio Candido
Authors: Otsuka Edu Teruki
Published: October 2024

Crítica fora do esquadro: homenagem a Antonio Candido.
Prenda que se ganha em lamento
Authors: Oliveira Marcelo Freitas Ferreira de
Published: October 2024

Após a publicação de A rosa do povo, em 1945, houve uma notória inflexão temática e estilística na poesia de Drummond, em cuja produção se passa a notar certo distanciamento face aos acontecimentos contemporâneos e às referências histórico-sociais, elaborando-se, a partir daí, uma poética voltada a matérias mais abstratas, de caráter universalizante ou metafísico. Nesse período há ainda o aprofundamento de um comportamento melancólico do sujeito poético amoroso drummondiano, em cujo canto se intensifica a alusão à ambiguidade e ao caráter fantasmático do amor. Neste ensaio apresentamos uma análise do poema “Escada”, de Fazendeiro do ar (1955), em que se lê o movimento de interpretação da experiência amorosa enquanto fantasmagoria potencial. Pois cabe quase sempre ao amante, amando, a consciência da perda, fazendo do poema amoroso um canto elegíaco, em que, ambiguamente, o amor e o poema só podem ser experimentados em sua precariedade e convite à dissolução, à fantasmagoria.
Diário da queda (trecho)
Authors: Laub Michel
Published: October 2024

Trechos de Diário da Queda, de Michel Laub.
Eighty years later: a new Neotropical species of Megalopelma Enderlein (Mycetophilidae) from the Cerrado and its conservation units
Authors: AFIUNE GIOVANA P. S. , OLIVEIRA SARAH S.
Published: October 2024

The genus Megelopelma was erected by Enderlein in 1910 for a species from Nova Teutônia district, Seara municipality in Santa Catarina state, Brazil. Edwards, in 1940, added three Neotropical species to the genus and no more species have been described so far, nor with the recent Diptera surveys in the Neotropics, which did not sample the genus. With a distance of more than 1,000 km from the genus type-locality and more than eighty years since the last species was described, we present a new species of Megalopelma from conservation units of Brazilian Cerrado, the central area of the Neotropical region, including photos, a distribution map, and a brief discussion regarding its potential use as a biological indicator for long-term monitoring.
Revision of the genus Goniopsara Metcalf, 1952 (Hemiptera: Fulgoroidea: Nogodinidae), with the description of a new species from Laos
Authors: GNEZDILOV VLADIMIR M.
Published: October 2024

The nogodinid genus Goniopsara Metcalf, 1952 is revised. The type species of the genus, Goniopsara mystica (Melichar, 1899), is redescribed and illustrated along with male genitalia structure is examined and described for the first time. A new species, Goniopsara ridens sp. nov., is described from Laos. A key to species of Goniopsara is given and the relatioships of this genus with Goniopsarites Meng, Wang et Wang, 2014 is briefly discussed.
A new species and new record of Nemourinae (Plecoptera: Nemouridae) from China
Authors: LI MENGYU , YANG DING , LI WEIHAI , MO RAORAO
Published: October 2024

A new species of the genus Illiesonemoura Baumann, 1975, I. lulang sp. nov., is described from Xizang, southwestern China, and compared with a related congener. Zapada quadribranchiata (Zhiltzova, 1977) is recorded from China for the first time.
Self-care strategies used by disaster responders after the 2023 earthquake in Turkey and Syria: a mixed methods study
Authors: Blomberg Karin , Murphy Jason , Hugelius Karin
Published: October 2024

Background

Disaster responders are exposed to several physical and mental health risks. This study aimed to describe self-care strategies used by disaster responders after the earthquake in Syria and eastern Turkey in February 2023.

Methods

A study specific web-based questionnaire survey was used to collect quantitative and qualitative data according to a convergent mixed methods approach. Data from 252 disaster responders responding to the earthquakes in Turkey and Syria were analyzed using both descriptive and analytical statistics and summative content analysis of free-text answers. Data were collected in March to July, 2023.

Results

The most used self-care strategies included resting, social support from colleagues in the field, extra intake of food or drink, and intake of medicines. The recovery strategies varied due to previous disaster response experience, indicating that supportive self-care strategies can be developed or learned.

Conclusion

Given the extreme conditions and limited possibilities of external support, sufficient self-care is an essential competence among disaster responders. Self-care strategies can be both external processed such as intake of medicines, social support from others, and internal processes such as personal reflection. Providing oneself with self-care activities seems to be a skill developed with increasing experience supported by pre-deployment training. Therefore, to enhance resilience, self-care strategies should be encompassed in pre-disaster response training.
Knowledge, perceptions, and feelings associated with Alzheimer’s disease and related dementias: a qualitative study among middle-aged latinas residing in an underserved agricultural community in California
Authors: Ambriz Elizabeth , De Pierola Camila , Norma Morga C. , Calderon Lucia , Kogut Katherine , Deardorff Julianna , Torres Jacqueline M.
Published: October 2024

Background

Middle age is increasingly acknowledged as a critical window for prevention of Alzheimer’s disease and related dementia (ADRD) since research has shown that AD develops in the course of 20–30 years (1) but we know very little about middle-aged individuals’ perspectives on ADRD. Knowledge gaps are particularly large for Latinas living in regions typically underrepresented in ADRD research, such as rural and/or agricultural regions. This is important given that over the next 40 years Latinos are projected to have the largest increase in ADRD cases in the U.S. Therefore, this study aims to assess knowledge, perceptions, and feelings associated with ADRD among a sample of middle-age, Spanish-speaking Latina women.

Method

Using qualitative methods involving semi-structured interviews, we examined knowledge, perceptions, and feelings associated with ADRD among a subsample of the Center for Health Assessment of Mothers and Children of Salinas (CHAMACOS) study. Participants are Latina women residing in an underserved agricultural community entering mid-life (mean = 46.5 years old). Interviews were conducted with 20 women and data was analyzed with inductive thematic content analysis.

Results

We identified themes regarding perceptions, knowledge, and feelings. First, participants perceive ADRD as involving (1) Loss of memory, (2) Getting lost; (3) Losing the person they once were. With regard to knowledge about ADRD, participants reported: (1) Some knowledge about protective and risk factors for ADRD, (2) No awareness of the links between cardiovascular risk factors and ADRD; (3) A desire to learn prevention methods alongside signs and symptoms of ADRD. Themes related to feelings about ADRD were: (1) Fear of developing ADRD and not being aware of reality or who they are; (2) Worry about losing relationships with loved ones and caretaking if diagnosed with ADRD; (3) Sadness about forgetting one’s family and depending on others if diagnosed with ADRD.

Conclusion

The knowledge gaps and negative feelings associated with ADRD highlighted in this study underscore the need for ADRD interventions to include CVD prevention, particularly for mid-life Latino populations residing in rural regions.
Coincidence of HCV and chronic kidney disease-a systematic review and meta-analysis
Authors: Nawaz Rabia , Ahmad Muhammad , Raza Muhammad Saad , Rashad Muhammad , Nawaz Ayesha , Tabassum Khadija , Hassan Jalees Ul , Ahad Ammara , Idrees Muhammad
Published: October 2024

Background

There are reported studies of Hepatitis C and chronic kidney disease association. However, how this liver virus infection affects the general population’s susceptibility to the onset of the kidney disease is still unknown.

Methods

To determine if a positive anti-HCV serologic status is linked to a greater incidence of chronic kidney disease in the general adult population, a systematic evaluation of the published medical literature since 2015 was conducted. A summary estimate of the relative risk of chronic kidney disease with HCV was produced using a random-effects model. Moreover, stratified analysis and meta-regression were performed.

Results

Twelve studies (n = 605858 patients) were filtered and included. Meta-analyses were conducted according to the outcome. Pooling results of longitudinal studies (n = 06 studies, n = 347120 unique patients) demonstrated an association between positive anti-HCV serologic status and increased incidence of CKD. The summary estimate for adjusted hazard ratio was 1.21 with (95% confidence interval 1.13; 1.29, P = 0.001), and between studies heterogeneity was noted (P value by Q test < 0.001). In the subset of Asian surveys, the risk of the occurrence of chronic kidney disease linked to HCV was 1.70 (95% confidence interval 1.40; 2.00) without heterogeneity (P value by Q test = 0.6).

Conclusions

We found a strong correlation between HCV infection and a higher risk of chronic renal disease in general global population.
Association between fish oil and glucosamine use and mortality in patients diagnosed with cancer: the role of the Life Essential 8 score and cancer prognosis
Authors: Lam Chun Sing , Hua Rong , Loong Herbert Ho-Fung , Chung Vincent Chi-Ho , Cheung Yin Ting
Published: October 2024

Background

The effect of supplements on mortality risk in patients with cancer remains uncertain and has scarcely been investigated in subgroups of patients with varying characteristics. This study aimed to investigate the association between two popular supplements, fish oil and glucosamine, and mortality risk in a large population-based cohort and determine whether cardiovascular health and clinical prognosis influence these associations.

Methods

This prospective cohort study analyzed the data of UK Biobank participants who were diagnosed with cancer. The associations of fish oil and glucosamine consumption with mortality were analyzed using Cox proportional hazards models. Subgroup analyses were performed to assess the effects of Life Essential 8 [LE8] scores (a measure of cardiovascular health) and cancer prognosis (grouped according to the survival rates of specific cancer types) on the associations between supplement use and mortality.

Results

This analysis included 14,920 participants (mean age = 59.9 years; 60.2% female). One third (34.1%) of the participants reported using fish oil, and one fifth (20.5%) reported using glucosamine. Over a median follow-up of 12.0 years, 2,708 all-cause deaths were registered. The use of fish oil was associated with reduced risks of all-cause mortality (adjusted hazard ratio [aHR] = 0.89, 95% Confidence Interval [CI] = 0.81–0.97) and cancer mortality (aHR = 0.89, 95% CI = 0.81–0.98). Similarly, glucosamine use was associated with reduced risks of all-cause mortality (aHR = 0.83, 95% CI = 0.74–0.92) and cancer mortality (aHR = 0.83, 95% CI = 0.74–0.93) in the fully adjusted model. Subgroup analyses revealed that the protective effects of fish oil and glucosamine against mortality risk were only observed in patients with LE8 scores lower than the mean score or a poor cancer prognosis. Additionally, the association between glucosamine use and a reduced risk of CVD-related mortality was only observed in patients with lower LE8 scores.

Conclusions

This large cohort study identified the potential differential impact of LE8 scores and cancer prognosis on the associations of fish oil and glucosamine supplementation with survival in patients with cancer. This suggests the importance of considering these factors in future research on supplements and in the provision of personalized integrative cancer care.
Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR: ABL1 signaling
Authors: Lebon Chiara , Grossmann Sebastian , Mann Greg , Lindner Florian , Koide Akiko , Koide Shohei , Diepold Andreas , Hantschel Oliver
Published: October 2024

Background

The inability of biologics to pass the plasma membrane prevents their development as therapeutics for intracellular targets. To address the lack of methods for cytosolic protein delivery, we used the type III secretion system (T3SS) of Y. enterocolitica, which naturally injects bacterial proteins into eukaryotic host cells, to deliver monobody proteins into cancer cells. Monobodies are small synthetic binding proteins that can inhibit oncogene signaling in cancer cells with high selectivity upon intracellular expression. Here, we engineered monobodies targeting the BCR::ABL1 tyrosine kinase for efficient delivery by the T3SS, quantified cytosolic delivery and target engagement in cancer cells and monitored inhibition of BCR::ABL1 signaling.

Methods

In vitro assays were performed to characterize destabilized monobodies (thermal shift assay and isothermal titration calorimetry) and to assess their secretion by the T3SS. Immunoblot assays were used to study the translocation of monobodies into different cell lines and to determine the intracellular concentration after translocation. Split-Nanoluc assays were performed to understand translocation and degradation kinetics and to evaluate target engagement after translocation. Phospho flow cytometry and apoptosis assays were performed to assess the functional effects of monobody translocation into BCR:ABL1-expressing leukemia cells.

Results

To enable efficient translocation of the stable monobody proteins by the T3SS, we engineered destabilized mutant monobodies that retained high affinity target binding and were efficiently injected into different cell lines. After injection, the cytosolic monobody concentrations reached mid-micromolar concentrations considerably exceeding their binding affinity. We found that injected monobodies targeting the BCR::ABL1 tyrosine kinase selectively engaged their target in the cytosol. The translocation resulted in inhibition of oncogenic signaling and specifically induced apoptosis in BCR::ABL1-dependent cells, consistent with the phenotype when the same monobody was intracellularly expressed.

Conclusion

Hence, we establish the T3SS of Y. enterocolitica as a highly efficient protein translocation method for monobody delivery, enabling the selective targeting of different oncogenic signaling pathways and providing a foundation for future therapeutic application against intracellular targets.
Transmitted drug resistance and molecular transmission network among treatment-naive HIV-1 patients in Wenzhou, China, 2020–2023
Authors: Zhang Tianran , Dou Huifen , Ye Hui , Tang Han , Wang Weiqin , Hu Wenxue , Lv Binbin , Zhou Mingshi , Dai Hupiao , Wang Weilong , Sun Baochang
Published: October 2024

Background

Transmitted drug resistance (TDR) increases the risk of antiretroviral therapy (ART) failure in HIV-1 patients. This study investigated the molecular epidemiology of TDR and its transmission networks among newly diagnosed HIV-1 patients in Wenzhou, China.

Methods

We enrolled 1878 ART-naive HIV-1 patients from January 2020 to October 2023. TDR was evaluated using the Stanford University HIV Drug Resistance Database. We performed phylogenetic analysis, genotyping, transmission clustering, and population-based TDR-related factor analysis.

Results

Among 1782 patients with successful genotyping, TDR prevalence was 5.7%. Multivariable analysis identified CRF08_BC subtype (adjusted odds ratio [aOR] 18.59, 95% CI 3.79-336.18, p = 0.004), CD4 > 500 cells/mm³ (aOR 2.19, 95% CI 1.16–4.03, p = 0.013), and year 2023 (aOR 1.83, 95% CI 1.11–4.89, p = 0.039) as factors associated with higher TDR risk. The most prevalent NNRTI mutations were K103N, E138A, and V179E. Seven TDR transmission clusters were identified, notably one with V179D that expanded during 2020–2023.

Conclusions

While TDR prevalence in Wenzhou remained lower than in other Chinese regions, an upward trend was observed. Most resistant individuals were in transmission clusters, predominantly middle-aged and elderly. NNRTI resistance was severe and concentrated in efavirenz, nevirapine, and rilpivirine. Enhanced HIV surveillance and wider free antiretroviral options are crucial to control drug-resistant HIV spread in Wenzhou.
Role of migratory birds as a risk factor for the transmission of multidrug resistant Salmonella enterica and Escherichia coli to broiler poultry farms and its surrounding environment
Authors: Tawakol Maram M. , Nabil Nehal M. , Samir Abdelhafez , Hassan Heba M. , Reda Reem M. , Abdelaziz Ola , Hagag Sahar , Elsayed Mona M.
Published: October 2024

Multidrug resistance (MDR) considered as global crisis facing poultry industry. Migratory birds play very important role in the dissemination of antimicrobial resistant pathogen during their fly way specially to poultry farms. Therefore, 750 samples from migratory birds and 300 samples from broiler chicken farms and its environment were collected during the winter seasons of five years (2019 to 2023). The samples were subjected to the isolation of Salmonella enterica and Escherichia coli with the detection of antimicrobial resistance (phenotypic and genotypic) with insight to the genetic similarity between the isolates from migratory birds and broiler chickens’ farms. Different members of Enterobacteriaceae were isolated; Salmonella enterica, Escherichia coli, Citrobacter, Enterobacter, Klebsiella, Proteus, Providencia, Serratia, Hafnia. 298 (28.4%) of S. enterica strains belonging to 27 serovars. S. Typhimurium, S. Kentucky, S. Enteritidis and S. Shangani were the common 4 serotypes between migratory birds and farms. Meanwhile, we found 489 (46.6%) isolates of E. coli belonging to 24 serogroups and O91, O128, O26, O125, O55, O103 and O159 were the common 7serogroups between migratory birds and farms samples. The majority of Salmonella (91.6%; 274 out of 298) and E. coli (92%; 450 out of 489) were MDR. The MDRI range of Salmonella and E. coli was 0.08- 1.The genetic similarity between the isolates of migratory birds and broiler chicken farms were detected by ERICPCR and hot map. This study suggests the continuous applications of surveillance programs for migratory birds and biosecurity measures in poultry farms.
Applications of ChatGPT in the diagnosis, management, education, and research of retinal diseases: a scoping review
Authors: Bellanda Victor C. F. , Santos Mateus Lins dos , Ferraz Daniel Araujo , Jorge Rodrigo , Melo Gustavo Barreto
Published: October 2024

Purpose

This scoping review aims to explore the current applications of ChatGPT in the retina field, highlighting its potential, challenges, and limitations.

Methods

A comprehensive literature search was conducted across multiple databases, including PubMed, Scopus, MEDLINE, and Embase, to identify relevant articles published from 2022 onwards. The inclusion criteria focused on studies evaluating the use of ChatGPT in retinal healthcare. Data were extracted and synthesized to map the scope of ChatGPT’s applications in retinal care, categorizing articles into various practical application areas such as academic research, charting, coding, diagnosis, disease management, and patient counseling.

Results

A total of 68 articles were included in the review, distributed across several categories: 8 related to academics and research, 5 to charting, 1 to coding and billing, 44 to diagnosis, 49 to disease management, 2 to literature consulting, 23 to medical education, and 33 to patient counseling. Many articles were classified into multiple categories due to overlapping topics. The findings indicate that while ChatGPT shows significant promise in areas such as medical education and diagnostic support, concerns regarding accuracy, reliability, and the potential for misinformation remain prevalent.

Conclusion

ChatGPT offers substantial potential in advancing retinal healthcare by supporting clinical decision-making, enhancing patient education, and automating administrative tasks. However, its current limitations, particularly in clinical accuracy and the risk of generating misinformation, necessitate cautious integration into practice, with continuous oversight from healthcare professionals. Future developments should focus on improving accuracy, incorporating up-to-date medical guidelines, and minimizing the risks associated with AI-driven healthcare tools.
A retrospective cohort analysis comparing the effectiveness and safety of perioperative angiotensin II to adrenergic vasopressors as a first-line vasopressor in kidney transplant recipients
Authors: Urias George , Benken Jamie , Nishioka Hokuto , Benedetti Enrico , Benken Scott T.
Published: October 2024

Background

Perioperative adrenergic vasopressors in kidney transplantation have been linked to negative outcomes and arrhythmias. Synthetic angiotensin II (AT2S) could improve renal hemodynamics, preserve allograft function, and reduce arrhythmias.

Objective

We aimed to compare the effectiveness and safety of AT2S to adrenergic vasopressors when used for perioperative hypotension in kidney transplant.

Methods

This single-center, retrospective cohort study included adults with perioperative shock requiring AT2S or adrenergic agents as first-line vasopressors during kidney transplant. The primary outcome was the need for a second continuous infusion vasopressor agents beyond the first-line agent. Secondary outcomes assessed adverse events and early allograft outcomes.

Results

Twenty patients receiving AT2S and 60 patients receiving adrenergic vasopressor agents were included. Intraoperatively, 1 of 20 patients (5%) in the AT2S group needed a second continuous vasopressor compared to 7 of 60 patients (11.7%) who needed a second continuous vasopressor in the adrenergic vasopressor group (P = 0.672). Postoperatively, 1 of 20 patients (5%) in the AT2S group compared to 12 of 60 patients (20%) in the adrenergic vasopressor group required a second vasopressor (P = 0.168). There were significantly fewer arrhythmias (1/20 [5%] vs. 17/60 [28.3%]), P = 0.03) and ischemic complications (0/20 [0%] vs. 11/20 [18.3%], P = 0.031) in patients who received AT2S. There were no differences in immediate, slow, or delayed graft function or in discharge, 1-month, and 3-month glomerular filtration rates (p > 0.05).

Conclusion and Relevance

Both AT2S and adrenergic vasopressors are effective for perioperative hypotension in kidney transplant, with AT2S showing a lower incidence of arrhythmias and ischemic complications.
Delays in Presentation, Diagnosis, and Treatment Among Patients With GI Cancer in Southwest Nigeria
Authors: Onwuka Justina Ucheojor , Wuraola Funmilola Olanike , Owoade Israel Adeyemi , Ogunyemi Yetunde Florence , Di Bernardo Matteo , Dare Anna J. , Mohammed Tajudeen Olakunle , Sheikh Mahdi , Olasehinde Olalekan , Kingham T. Peter , Robbins Hilary A. , Alatise Olusegun Isaac
Published: October 2024

PURPOSE

The incidence of GI cancers is increasing in sub-Saharan African countries. We described the oncological care pathway and assessed presentation, diagnosis, and treatment intervals and delays among patients with GI cancer who presented to the Obafemi Awolowo University Teaching Hospitals Complex in Ile-Ife, Nigeria.

METHODS

We analyzed data from 545 patients with GI cancer in the African Research Group for Oncology (ARGO) database. We defined presentation interval as the interval between symptom onset and presentation to tertiary hospital, diagnostic interval as between presentation and diagnosis, and treatment interval as between diagnosis and initiation of treatment. We considered >3 months, >1 month, and >1 month to be presentation, diagnosis, and treatment delays, respectively. We compared lengths of intervals using Mann-Whitney
U
tests and logistic regression.

RESULTS

The most frequent cancer types were pancreatic (32%) and colorectal (28%). Most patients presented at stages III (38%) and IV (30%). The median presentation interval was 84 days (IQR, 56-191), and 49% presented after 3 months or longer. The median diagnosis and treatment intervals were 0 (IQR, 0-8) and 7 (IQR, 0-23) days, respectively. There was no relationship between age, sex, education, or distance to tertiary hospital and presentation delay, but patients with stage III to IV versus I to II had higher odds of presentation delay (odds ratio [OR], 1.68 [95% CI, 1.13 to 2.50]). Among patients with pancreatic cancer, older patients were less likely to have a diagnosis delay (OR, 0.50 [95% CI, 0.25 to 0.98]).

CONCLUSION

About half of patients with GI cancer in Ile-Ife, Nigeria, did not present to tertiary hospitals until more than 90 days after noticing symptoms. Efforts are warranted to improve public knowledge of GI cancer symptoms and to strengthen health systems for prompt diagnosis and referral to specialty care.
Influence of the ionic electric field on Stark shifts of neutral helium lines in plasmas
Authors: Amieur B. , Meftah M. T. , Chenini K. , Nana Y. Ben , Guerrida H.
Published: October 2024

In this work we provide a contribution to Stark shift for some lines of neutral helium in plasmas. The mentioned shift is caused by the collisions of the unbounded electrons with helium atoms in plasmas. We calculate this contribution by taking into account only weak collisions. This contribution takes into consideration the presence of the local electric microfield created by the ions that constitute in part the plasma. The effect of this microfield modifies the electron trajectory and therefore changes the shift formula developed early by using the straight trajectories in the case of neutral emitters. Our new contribution to the Stark shift caused by electron's collision is applied to some HeI lines: 3188

, 3888

, 6678

and 7065

. Our results are confronted with the experimental and theoretical data from the literature which clearly show that the influence of the local electric microfield created by the ions on the Stark shift caused by electron's collision is not negligible and must be taken into account. It turns out that the correction to the theoretical shift for the lines under consideration tends to red.
Effect of classical laryngeal mask or I-gel use on otolaryngeal system in ambulatory inhalation general anesthesia: A prospective study
Authors: Saylan Sedat , Cobanoglu Hatice Bengu Yaldiz
Published: October 2024

Objective: To investigate the effects of supraglottic airway tools such as classical laryngeal mask (cLMA) and I-gel, which can be used without the need for muscle relaxation in the airway management of general anesthesia patients, on the otolaryngeal system.
Methods: This prospective randomised study was conducted at Karadeniz Technical University Hospital, Faculty of Medicine, Trabzon, Turkey, during November 2020 to December 2021. Eighty-nine patients in the American Society of Anesthesiologists (ASA) grade I-II group, who would undergo elective surgery under general anesthesia, were randomized into two groups, namely Group cLMA and Group I-gel. cLMA and I-gel were used for airway management of the patients. After anesthesia induction, tympanometric measurements were taken at regular intervals for middle ear pressures of both ears.
Results: While air way pressures, SpO2 and EtCO2 values were within normal limits, there were no differences in terms of complications. In tympanometric measurements, middle ear pressure increase was statistically higher in the cLMA group than in the I-gel group (p <0.001).
Conclusions: We think that I-gel may be a more advantageous supra glottic airway device in terms of otolaryngeal effecs and middle ear pressure in theair way management during short surgical procedures.
doi: https://doi.org/10.12669/pjms.40.10.8416
How to cite this: Saylan S, Cobanoglu HBY. Effect of classical laryngeal mask or I-gel use on otolaryngeal system in ambulatory inhalation general anesthesia: A prospective study. Pak J Med Sci. 2024;40(10):2299-2304. doi: https://doi.org/10.12669/pjms.40.10.8416
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Impact and outcomes of hyperglycemia among post-operative children who underwent repair of congenital cardiac defects in a tertiary care setup of Pakistan
Authors: Yumna Aslam  , Ayaz Ur Rehman  , Tarab Zehra  , Noor Ulain
Published: October 2024

Objective: To determine frequency of postoperative hyperglycemia in children who underwent repair of Congenital Cardiac Defects (CCDs) in a tertiary care hospital of Pakistan. The other objective was to compare outcomes in children with versus without postoperative hyperglycemia who underwent repair of CCDs in a tertiary care hospital of Pakistan.
Methods: A descriptive study was conducted at the Cardiac Intensive Care Unit (CICU), Aga Khan University Hospital (AKUH), Karachi, Pakistan from October 3, 2020, to April 2, 2021. A total of 185 patients aged one month-18 years admitted to CICU underwent elective surgical repair of CCDs meeting the eligibility criteria were included. Glucose monitoring was collected at immediate, 4th and 8th postoperative hours on the first day and once daily on postoperative days Two and Three to assess hyperglycemia. Data including age, weight, gender, pre-operative diagnosis, type of surgery and variables such as CICU stay, duration of hospital stay and duration of mechanical ventilation were recorded.
Results: The mean age of the patients was 40.40±48.63 months. Out of 185 patients, 115 (62.2%) were male while 70 (37.8%) were female. Hyperglycemia was found to be in 182 (98.4%) patients. Out of 182 hyperglycemic patients, 53 (28.6%) had moderate hyperglycemia while 129 (69.7%) had severe hyperglycemia. Mean duration of hospital stays was 9.24±7.45, mean duration of pediatric CICU stays was 141.61±169.02 hours and mean duration of mechanical ventilation was 80.59±98.54 hours.
Conclusion: Hyperglycemia is a frequent finding in children who underwent repair of CCDs, and it is not significantly associated with duration of mechanical ventilation, length of hospital and CICU stay.
doi: https://doi.org/10.12669/pjms.40.10.8763
How to cite this: Aslam Y, Ayaz Ur Rehman, Zehra T, Noor Ul Ain. Impact and outcomes of hyperglycemia among post-operative children who underwent repair of congenital cardiac defects in a tertiary care setup of Pakistan. Pak J Med Sci. 2024;40(10):2277-2280.  doi: https://doi.org/10.12669/pjms.40.10.8763
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Effect of educational interventions addressing blood groups and donation aspects on the knowledge and attitude of allied health sciences students
Authors: Saqlain Nazish , Habib Hafsa , Riaz Shazia , Hareem Sidra
Published: October 2024

Objective: To determine the effect of educational interventions on knowledge and approach of Allied Health Sciences Students regarding blood groups and blood donation.
Methods: It was a cross-sectional study conducted at the department of Hematology and Transfusion Medicine, University of Child Health Sciences, The Children’s Hospital, Lahore from October 2022-January 2023. A self-designed questionnaire was used to assess the baseline knowledge of students followed by educational interventions in the form of small group discussions, video lectures and practical demonstrations. Post-interventions assessment was done using the same questionnaire 30 days later. Data obtained was analyzed using SPSS-23 software. A p-Value <0.05 was considered significant. The reliability of the questionnaire was checked after the pilot study by using the SPSS-23 software and calculated Cronbach’s Alpha was 0.82.
Results: Of the 99 participants, there were 13 males and 86 females with mean age of 21.41±1.478 years. Pre-interventions, majority participants had moderate knowledge (81.8%). Medical Lab technologists showed better baseline knowledge than the rest. Post-interventions, the level of knowledge among students increased to 94.9%. Pre-interventions, knowledge about blood groups and donation ranged from 14.1-79% and 25.5-77.8% correct responses respectively while post-interventions it went up to 92-100% for both aspects. The willingness to donate blood in future raised from 46.4% to 92.9%.
Conclusions: Educational interventions were effective in improving the knowledge of Allied Health Sciences students about blood groups and blood donation. The enlightenment strategies positively influenced students towards the active participation in blood donation campaigns.
doi: https://doi.org/10.12669/pjms.40.10.8803
How to cite this: Saqlain N, Habib H, Riaz S, Hareem S. Effect of educational interventions addressing blood groups and donation aspects on the knowledge and attitude of allied health sciences students . Pak J Med Sci. 2024;40(10):2261-2266. doi: https://doi.org/10.12669/pjms.40.10.8803
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.