bluuebunny/crossref_metadata_2025 huggingface dataset:
Surgery in times of COVID-19—recommendations for hospital and patient management
Authors: Flemming S. , Hankir M. , Ernestus R.-I. , Seyfried F. , Germer C.-T. , Meybohm P. , Wurmb T. , Vogel U. , Wiegering A.
Published: May 2020

Background

The novel coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has escalated rapidly to a global pandemic stretching healthcare systems worldwide to their limits. Surgeons have had to immediately react to this unprecedented clinical challenge by systematically repurposing surgical wards.

Purpose

To provide a detailed set of guidelines developed in a surgical ward at University Hospital Wuerzburg to safely accommodate the exponentially rising cases of SARS-CoV-2 infected patients without compromising the care of emergency surgery and oncological patients or jeopardizing the well-being of hospital staff.

Conclusions

The dynamic prioritization of SARS-CoV-2 infected and surgical patient groups is key to preserving life while maintaining high surgical standards. Strictly segregating patient groups in emergency rooms, non-intensive care wards and operating areas prevents viral spread while adequately training and carefully selecting hospital staff allow them to confidently and successfully undertake their respective clinical duties.
Acquired resistance to osimertinib in patients with non-small-cell lung cancer: mechanisms and clinical outcomes
Authors: Mu Yuxin , Hao Xuezhi , Xing Puyuan , Hu Xingsheng , Wang Yan , Li Teng , Zhang Jinyao , Xu Ziyi , Li Junling
Published: May 2020

Purpose

Osimertinib, a third-generation epidermal growth factor receptor tyrosine-kinase inhibitor (EGFR-TKI), has demonstrated substantial clinical benefit in patients with non-small-cell lung cancer (NSCLC) who were resistant to early-generation EGFR-TKIs and had acquired a T790M mutation. The aim of our study was to identify the mechanisms underlying resistance to osimertinib and to correlate them with clinical outcomes.

Methods

We retrospectively analyzed patients with advanced NSCLC who received osimertinib for T790M-mutated acquired resistance to prior EGFR-TKIs between March 1, 2017 and December 31, 2018. Patients with paired molecular data of pre-osimertinib and after resistance development, which were not confirmed with small-cell lung cancer (SCLC) transformation, were included in the molecular analysis set.

Results

Of 49 patients evaluated in the molecular analysis set, 24 patients maintained T790M mutation, while 25 patients exhibited T790M-loss. Molecular modifications were identified in 27 of 49 patients including EGFR acquired mutations (C797S, C796S, G796S, V802I, V834L, E758D and G724S), non-EGFR-dependent mutations (PIK3CA, ALK, BRAF, KRAS and TP53), EGFR amplification and MET amplification. At data cutoff, median progression-free survival (PFS) was 9.3 months in the T790M-retain group compared with 7.8 months in T790M-loss patients (P = 0.053). Median PFS was significantly longer in patients with EGFR-dependent resistance mechanism (13.5 months) than in those with alternative pathway activation (8.2 months; P = 0.012).

Conclusions

The study revealed heterogeneous mechanisms of resistance to osimertinib in advanced NSCLC patients and their association with clinical outcomes. Patients who maintained T790M mutation or with EGFR-dependent resistance mechanism had longer clinical outcome benefits.
Development and initial evaluation of a novel simulation model for comprehensive brain tumor surgery training
Authors: Grosch Anne Sophie , Schröder Timo , Schröder Torsten , Onken Julia , Picht Thomas
Published: May 2020

Background

Increasing technico-manual complexity of procedures and time constraints necessitates effective neurosurgical training. For this purpose, both screen- and model-based simulations are under investigation. Approaches including 3D printed brains, gelatin composite models, and virtual environments have already been published. However, quality of brain surgery simulation is limited due to discrepancies in visual and haptic experience. Similarly, virtual training scenarios are still lacking sufficient real-world resemblance. In this study, we introduce a novel simulator for realistic neurosurgical training that combines real brain tissue with 3D printing and augmented reality.

Methods

Based on a human CT scan, a skull base and skullcap were 3D printed and equipped with an artificial dura mater. The cerebral hemispheres of a calf’s brain were placed in the convexity of the skullcap and tumor masses composed of aspic, water, and fluorescein were injected in the brain. The skullcap and skull base were placed on each other, glued together, and filled up with an aspic water solution for brain fixation. Then, four surgical scenarios were performed in the operating room as follows: (1) simple tumor resection, (2) complex tumor resection, (3) navigated biopsy via burr hole trepanation, and (4) retrosigmoidal craniotomy. Neuronavigation, augmented reality, fluorescence, and ocular—as well as screen-based (exoscopic)—surgery were available for the simulator training. A total of 29 participants performed at least one training scenario of the simulator and completed a 5-item Likert-like questionnaire as well as qualitative interviews. The questionnaire assessed the realism of the tumor model, skull, and brain tissue as well as the capability for training purposes.

Results

Visual and sensory realism of the skull and brain tissue were rated,”very good,” while the sensory and visual realism of the tumor model were rated “good.” Both overall satisfaction with the model and eligibility of the microscope and neurosurgical instruments for training purposes were rated with “very good.” However, small size of the calf’s brain, its limited shelf life, and the inability to simulate bleedings due to the lack of perfusion were significant drawbacks.

Conclusion

The combination of 3D printing and real brain tissue provided surgical scenarios with very good real-life resemblance. This novel neurosurgical model features a versatile setup for surgical skill training and allows for efficient training of technological support like image and fluorescence guidance, exoscopic surgery, and robotic technology.
Do not keep it simple: recent advances in the generation of complex organoids
Authors: Wörsdörfer Philipp , I Takashi , Asahina Izumi , Sumita Yoshinori , Ergün Süleyman
Published: May 2020

3D cell culture models which closely resemble real human tissues are of high interest for disease modelling, drug screening as well as a deeper understanding of human developmental biology. Such structures are termed organoids. Within the last years, several human organoid models were described. These are usually stem cell derived, arise by self-organization, mimic mechanisms of normal tissue development, show typical organ morphogenesis and recapitulate at least some organ specific functions. Many tissues have been reproduced in vitro such as gut, liver, lung, kidney and brain. The resulting entities can be either derived from an adult stem cell population, or generated from pluripotent stem cells using a specific differentiation protocol. However, many organoid models only recapitulate the organs parenchyma but are devoid of stromal components such as blood vessels, connective tissue and inflammatory cells. Recent studies show that the incorporation of endothelial and mesenchymal cells into organoids improved their maturation and might be required to create fully functional micro-tissues, which will allow deeper insights into human embryogenesis as well as disease development and progression. In this review article, we will summarize and discuss recent works trying to incorporate stromal components into organoids, with a special focus on neural organoid models.
CDK7 inhibitors as anticancer drugs
Authors: Sava Georgina P. , Fan Hailing , Coombes R. Charles , Buluwela Lakjaya , Ali Simak
Published: May 2020

Cyclin-dependent kinase 7 (CDK7), along with cyclin H and MAT1, forms the CDK-activating complex (CAK), which directs progression through the cell cycle via T-loop phosphorylation of cell cycle CDKs. CAK is also a component of the general transcription factor, TFIIH. CDK7-mediated phosphorylation of RNA polymerase II (Pol II) at active gene promoters permits transcription. Cell cycle dysregulation is an established hallmark of cancer, and aberrant control of transcriptional processes, through diverse mechanisms, is also common in many cancers. Furthermore, CDK7 levels are elevated in a number of cancer types and are associated with clinical outcomes, suggestive of greater dependence on CDK7 activity, compared with normal tissues. These findings identify CDK7 as a cancer therapeutic target, and several recent publications report selective CDK7 inhibitors (CDK7i) with activity against diverse cancer types. Preclinical studies have shown that CDK7i cause cell cycle arrest, apoptosis and repression of transcription, particularly of super-enhancer-associated genes in cancer, and have demonstrated their potential for overcoming resistance to cancer treatments. Moreover, combinations of CDK7i with other targeted cancer therapies, including BET inhibitors, BCL2 inhibitors and hormone therapies, have shown efficacy in model systems. Four CDK7i, ICEC0942 (CT7001), SY-1365, SY-5609 and LY3405105, have now progressed to Phase I/II clinical trials. Here we describe the work that has led to the development of selective CDK7i, the current status of the most advanced clinical candidates, and discuss their potential importance as cancer therapeutics, both as monotherapies and in combination settings. ClinicalTrials.gov Identifiers: NCT03363893; NCT03134638; NCT04247126; NCT03770494.
The effect of subacute poisoning with fenpropathrin on mice kidney function and the level of interleukin 1β and tumor necrosis factor α
Authors: Jaremek Maria , Nieradko-Iwanicka Barbara
Published: May 2020

Fenpropathrin (FEN) is a pyrethroid insecticide. Mammals can be exposed to these compounds with food and water as non-target organisms. Pyrethroids are classified into two types depending on chemical structure and neurotoxic effects. FEN has features of Type I and Type II pyrethroids. There is data that pyrethroids apart from neurotoxic properties, can be also nephrotoxic and immunotoxic. The aim of the study was to assess the influence of FEN on kidney function and concentration of proinflammatory cytokines: tumor necrosis factor alpha (TNFα) and interleukin 1 beta (IL-1β) in mice kidneys. Sixteen female mice were randomly divided into two groups: I—receiving saline and II—receiving FEN at the dose of 11.9 mg/kg ip for 28 consecutive days. On day 29 blood samples were obtained to measure serum creatinine concentration. The animals were killed, and kidneys were obtained in order to measure TNFα and interleukin IL-1β in mice kidneys with use of ELISA assay. The concentration of creatinine was (mean ± SD) in controls 0.2 ± 0.0 mg/dl in the group exposed to FEN 0.225 ± 0.046 mg/dl. TNFα concentration in the kidneys of controls was 6.154 ± 1.597 pg/ml and in the group intoxicated with FEN it was 6.318 ± 1.012 pg/ml. IL-1β concentration in the kidneys of controls was 4.67 ± 1.154 pg/ml while in the group intoxicated with FEN 27.983 ± 26.382 pg/ml (p < 0.05). In conclusion: FEN slightly affects kidney function and increases the concentration of proinflammatory IL-1β in mice kidneys, which supports the hypothesis about nephrotoxic and immunotoxic effect of this insecticide in non target organisms.
Culturally embedded schemata for false belief reasoning
Authors: Berio Leda
Published: May 2020

I argue that both language acquisition and cultural and social factors contribute to the formation of schemata that facilitate false belief reasoning. While the proposal for an active role of language acquisition in this sense has been partially advanced by several voices in the mentalizing debate, I argue that other accounts addressing this issue present some shortcomings. Specifically, I analyze the existing proposals distinguishing between “structure-oriented” views (with the most relevant representative in de Villiers and de Villiers in Complements enable representation of the contents of false beliefs: the evolution of a theory of theory of mind. Language Acquisition, Foster-Cohen, 2009; de Villiers in Int Rev Gen Linguistics 117(11):1858–1878, 2007; de Villiers in Why language matters for theory of mind. Oxford University Press, New York, pp 186–219, 2005), that stress the role of language as a set of rules providing syntactic input and providing a representational format, and “cultural/social-oriented views”, that stress the role of social interaction (among others Nelson in Why language matters for theory of mind. Oxford University Press, Oxford, 2005; Hutto in Folk psychological narratives: the sociocultural basis of understanding reasons. Cambridge University Press, Cambridge, 2008b). Starting from the analysis of these views, I defend my own account of the role of language acquisition in aiding false belief reasoning. I argue that language acquisition plays a pivotal role in the formation of schemata used by pre-schoolers to pass the false belief task. I propose a specific learning mechanism for exploiting linguistic information that taps into specific cognitive abilities, thus making a very concrete suggestion about the role of linguistic input in specific cultural contexts for the development of false belief skills.
Development and Application of a Multiplex Marker Assay to Detect PVY Resistance Genes in Solanum tuberosum
Authors: Elison Gregory L. , Hall Darren G. , Novy Richard G. , Whitworth Jonathan L.
Published: May 2020

Potato virus Y (PVY) is a major pathogen affecting potato production worldwide. Three independent genes (Ryadg,Rysto,and Rychc) conferring genetic resistance to all known strains of the virus are currently utilized by breeding programs to develop potato varieties containing extreme resistance to infection by PVY. These resistance genes are typically detected using primers specific for the generation of markers closely-linked to the resistance genes. In recent years there have been attempts to develop a multiplex PCR assay for all resistance genes, but all current published methods either omit at least one resistance gene or are unnecessarily complex and therefore difficult to incorporate successfully for marker-assisted selection. We have developed an assay which tests for the presence of all three sources of resistance in a single, easy to use PCR protocol. The multiplex PCR assay was applied to potato varieties, second-field year breeding germplasm, and a selection of test crosses having progeny segregating for multiple resistance genes to validate the methodology for use in marker-assisted selection in potato breeding programs.
Prefrontal Nectin3 Reduction Mediates Adolescent Stress-Induced Deficits of Social Memory, Spatial Working Memory, and Dendritic Structure in Mice
Authors: Wang Hong-Li , Li Ji-Tao , Wang Han , Sun Ya-Xin , Liu Rui , Wang Xiao-Dong , Su Yun-Ai , Si Tian-Mei
Published: May 2020

Chronic stress may disrupt the normal neurodevelopmental trajectory of the adolescent brain (especially the prefrontal cortex) and contribute to the pathophysiology of stress-related mental illnesses, but the underlying molecular mechanisms remain unclear. Here, we investigated how synaptic cell adhesion molecules (e.g., nectin3) are involved in the effects of adolescent chronic stress on mouse medial prefrontal cortex (mPFC). Male C57BL/6N mice were subjected to chronic social instability stress from postnatal days 29 to 77. One week later, the mice exposed to chronic stress exhibited impaired social recognition and spatial working memory, simplified dendritic structure, and reduced spine density in the mPFC. Membrane localization of nectin3 was also altered, and was significantly correlated with behavioral performance. Furthermore, knocking down mPFC nectin3 expression by adeno-associated virus in adolescent mice reproduced the stress-induced changes in behavior and mPFC morphology. These results support the hypothesis that nectin3 is a potential mediator of the effects of adolescent chronic stress on prefrontal structural and functional abnormalities.
Contribution of women’s fisheries substantial, but overlooked, in Timor-Leste
Authors: Tilley Alexander , Burgos Ariadna , Duarte Agustinha , dos Reis Lopes Joctan , Eriksson Hampus , Mills David
Published: May 2020

A greater understanding of gendered roles in fisheries is necessary to value the often-hidden roles that women play in fisheries and households. We examine women’s contributions to household food and income using focus group discussions, market surveys, and landings data in six communities in Timor-Leste. Women were actively fishing more days per month than men. Gleaning was the most frequent activity and 100% of trips returned with catch for food and/or income. Mollusc and crab catches were common and exploitation appeared targeted on a dynamic reappraisal of changing food values and changing estimates of group needs. With as many as 80% of households in coastal areas involved in fishing, and at least 50% of women fishing, this highlights the current lack of women’s engagement as a critical gap in fisheries management approaches. The current androcentric dialogue limits social-ecological understanding of these systems and the potential for their effective stewardship.
Flexibility Grave – Partial Reproduction Focus and Closed System Fetishism in CJEU, Pelham
Authors: Senftleben Martin
Published: May 2020

In the ongoing discussion about the impact of fundamental rights on EU copyright law, thePelhamjudgment of the Court of Justice of the European Union (CJEU) has received much attention. However, the decision also raises important legal-doctrinal issues. The CJEU employs the harmonized right of reproduction as a vehicle to regulate adaptations of pre-existing source material. Moreover, the Court insists on a balancing of interests within the EU matrix of exclusive rights and limitations. The closed list of limitations in EU copyright law, however, can hardly be expected to offer sufficient breathing space for adaptation scenarios. As the Information Society Directive did not harmonize the right of adaptation, there was no need to include indispensable free adaptation rules that have evolved at the national level, such as the German “free use” doctrine. Instead of embracing national rules of equity and fairness to fill the gap, the CJEU is reluctant to borrow from the legal traditions of EU Member States and misses an important opportunity to provide guidance for the regulation of adaptations outside the sound sampling arena. After an introduction to the German “Metall auf Metall” saga that led to thePelhamdecision, the following analysis sheds light on these developments in EU copyright law and discusses problems arising from the approach taken by the CJEU.
Provenance-Aware Knowledge Representation: A Survey of Data Models and Contextualized Knowledge Graphs
Authors: Sikos Leslie F. , Philp Dean
Published: May 2020

Expressing machine-interpretable statements in the form of subject-predicate-object triples is a well-established practice for capturing semantics of structured data. However, the standard used for representing these triples, RDF, inherently lacks the mechanism to attach provenance data, which would be crucial to make automatically generated and/or processed data authoritative. This paper is a critical review of data models, annotation frameworks, knowledge organization systems, serialization syntaxes, and algebras that enable provenance-aware RDF statements. The various approaches are assessed in terms of standard compliance, formal semantics, tuple type, vocabulary term usage, blank nodes, provenance granularity, and scalability. This can be used to advance existing solutions and help implementers to select the most suitable approach (or a combination of approaches) for their applications. Moreover, the analysis of the mechanisms and their limitations highlighted in this paper can serve as the basis for novel approaches in RDF-powered applications with increasing provenance needs.
Bildpräsenz
Authors: Grave Johannes
Published: May 2020

In vielen literarischen Texten umkreist Goethe problematische Effekte von Bildern und Bildpraktiken. Bilder können dazu einladen, das bildlich Dargestellte für einen Teil der Wirklichkeit zu halten, sie können Gegenstand fragwürdiger Projektionen werden oder aber dazu beitragen, die Wirklichkeit selbst bildhaft erstarren zu lassen. Diese Gefahren im Umgang mit Bildern sind nicht zuletzt auf deren spezifische temporale Verfasstheit zurückzuführen, insbesondere wenn die physische Präsenz eines Bildes genutzt wird, um Vergangenes oder Abwesendes zu vergegenwärtigen. Der Beitrag entfaltet diese Problemlage an verschiedenen exemplarischen Texten Goethes. Am Beispiel des Aufsatzes »Ruysdael als Dichter« skizziert er eine alternative Form des Umgangs mit Bildern, die nicht allein auf deren Präsenzeffekt setzt, sondern eine dem Bild eigene Temporalität im Prozess des Sehens und Reflektierens zur Geltung bringt.
Religiöse Identitäten und Vorurteil in Deutschland und der Schweiz – Konzeptionelle Überlegungen und empirische Befunde
Authors: Pickel Gert , Liedhegener Antonius , Jaeckel Yvonne , Odermatt Anastas , Yendell Alexander
Published: May 2020

Immer wieder werden Bezüge zwischen Religion und Vorurteilen hergestellt. Zum einen dienen religiöse Gruppen als Ziel von Vorurteilen, zum anderen wird debattiert, inwieweit der Wahrheitsanspruch von Religionen religiöse Menschen für Vorurteile anfälliger macht. Im Artikel werden mithilfe des aktuellen KONID Survey 2019 beide Fragen empirisch und Ländervergleichend untersucht. Trotz einzelner Abweichungen in Ausprägungen und Bezügen von Vorurteilen, sind die Strukturen der Vorurteile, ihre Erklärung sowie ihre demokratischen Auswirkungen in Deutschland und der Schweiz sehr ähnlich. Religionsgemeinschaften und ihre Mitglieder geraten vor allem aufgrund von Bedrohungsängsten in der Bevölkerung, aber auch Verschwörungstheorien und autoritären Einstellungen in den Fokus von Vorurteilen. Dabei wirkt eine dogmatische, exklusivistisch gedachte und teilweise fundamentalistische religiöse Identität als Vorurteile befördernd, während eine liberale religiöse Identität Antisemitismus, antimuslimischen Rassismus, aber auch Antiziganismus und die Ablehnung von Nichtbinären Geschlechteridentitäten entgegenwirkt.
Trade policy in a “GVC World”: Multinational corporations and trade liberalization
Authors: Anderer Christina , Dür Andreas , Lechner Lisa
Published: May 2020

The globalization of production is changing the political economy of trade policymaking: Traditional supporters of free trade (exporters seeking market access in foreign countries) are joined by new actors (companies needing intermediates from abroad for their production processes) in their lobbying efforts for trade liberalization. Multinational corporations (MNCs) play a crucial role in this new alliance due to their strong involvement in international trade and endowment with resources that can be used to lobby policymakers. We derive an argument from these premises that leads to the expectation of variation in trade policy outcomes across industries depending on their degree of integration in a global network of multinational corporations. Disaggregated data on the level of tariffs and speed of tariff cuts in preferential trade agreements, international mergers and acquisitions at the firm level, and MNC imports of intermediates by sector allow us to test the argument. The findings support our theoretical expectations. The paper sheds light on the processes and outcomes of trade policymaking in a globalized economy by further developing an existing argument about GVCs and trade policy outcomes as well as expanding on it by adding data on international corporate connections.
Respiratory surveillance wards as a strategy to reduce nosocomial transmission of COVID-19 through early detection: The experience of a tertiary-care hospital in Singapore
Authors: Wee Liang En , Hsieh Jenny Yi Chen , Phua Ghee Chee , Tan Yuyang , Conceicao Edwin Philip , Wijaya Limin , Tan Thuan Tong , Tan Ban Hock
Published: May 2020

Objectives:

Patients with COVID-19 may present with respiratory syndromes indistinguishable from those caused by common viruses. Early isolation and containment is challenging. Although screening all patients with respiratory symptoms for COVID-19 has been recommended, the practicality of such an effort has yet to be assessed.

Methods:

Over a 6-week period during a SARS-CoV-2 outbreak, our institution introduced a “respiratory surveillance ward” (RSW) to segregate all patients with respiratory symptoms in designated areas, where appropriate personal protective equipment (PPE) could be utilized until SARS-CoV-2 testing was done. Patients could be transferred when SARS-CoV-2 tests were negative on 2 consecutive occasions, 24 hours apart.

Results:

Over the study period, 1,178 patients were admitted to the RSWs. The mean length-of-stay (LOS) was 1.89 days (SD, 1.23). Among confirmed cases of pneumonia admitted to the RSW, 5 of 310 patients (1.61%) tested positive for SARS-CoV-2. This finding was comparable to the pickup rate from our isolation ward. In total, 126 HCWs were potentially exposed to these cases; however, only 3 (2.38%) required quarantine because most used appropriate PPE. In addition, 13 inpatients overlapped with the index cases during their stay in the RSW; of these 13 exposed inpatients, 1 patient subsequently developed COVID-19 after exposure. No patient–HCW transmission was detected despite intensive surveillance.

Conclusions:

Our institution successfully utilized the strategy of an RSW over a 6-week period to contain a cluster of COVID-19 cases and to prevent patient–HCW transmission. However, this method was resource-intensive in terms of testing and bed capacity.
The COVID-19 infection control arms race
Authors: Rhee Chanu , Baker Meghan A. , Klompas Michael
Published: May 2020

US hospitals are engaged in an infection control arms race. Hospitals, specialties, and professional groups are spurring one another on to adopt progressively more aggressive measures in response to COVID-19 that often exceed federal and international standards. Examples include universal masking of providers and patients; decreasing thresholds to test asymptomatic patients; using face shields and N95 respirators regardless of symptoms and test results; novel additions to the list of aerosol-generating procedures; and more comprehensive personal protective equipment including hair, shoe, and leg covers. Here, we review the factors underlying this arms race, including fears about personal safety, ongoing uncertainty around how SARS-CoV-2 is transmitted, confusion about what constitutes an aerosol-generating procedure, increasing recognition of the importance of asymptomatic infection, and the limited accuracy of diagnostic tests. We consider the detrimental effects of a maximal infection control approach and the research studies that are needed to eventually de-escalate hospitals and to inform more evidence-based and measured strategies.
The effect of the National Birth Defects Intervention Project on the prevention of congenital disabilities among children in China: a natural experiment
Authors: Guo Chao , Zheng Xiaoying
Published: May 2020

Most childhood disabilities are caused by congenital factors such as birth defects. The present study aims to evaluate the effect of periconceptional nutrition intervention on the prevention of congenital disability among Chinese children using the National Birth Defects Intervention Project as a natural experiment. We obtained individual-level data from the Second National Sample Survey on Disability, a nationally representative survey, and 110 365 children born between September 1999 and August 2003 were included for analysis. Difference-in-differences estimates of the project effects on congenital disability were captured by exploiting temporal variation in the timing of project exposure across four birth cohorts along with geographical variation in project category at the province level. The findings contribute to an emerging body of evidence showing that prenatal micronutrient intervention before and during early pregnancy could substantially reduce the risk of congenital disability in childhood (OR 0·73; 95 % CI 0·57, 0·94). The National Birth Defects Intervention Project improved the awareness of reproductive health and disability prevention in the population. It highlights the need for a potential policy change focusing on early-life health investment in China.
Identification and evaluation of the main risk periods of Botrytis cinerea infection on grapevine based on phenology, weather conditions and airborne conidia
Authors: González-Fernández E. , Piña-Rey A. , Fernández-González M. , Aira M. J. , Rodríguez-Rajo F. J.
Published: May 2020

In the present study, a new method for a decision-support system for fungicide administration against the pathogen Botrytis cinerea in vineyards was developed based on Integrated Pest Management principles which identified an infection risk before the appearance of disease symptoms. The proposed method is based on the combination of (i) the phenological observations of the main susceptible stages to infection, (ii) the airborne spores monitoring, (iii) the forecasting of the suitable meteorological conditions for B. cinerea spore germination during the subsequent 4–6 days after the spore detection. Aerobiological, phenological and meteorological analyses were carried out using data from 2008 to 2015 in a vineyard of Northwestern Spain. Aerobiological spore data were obtained using a Lanzoni VPPS-2000 pollen-spore trap. Phenological observations were conducted on 22 plants of Treixadura cultivar following the BBCH (Biologische Bundesanstalt für Land und Forstwirtschaft, Bundessortenamt und CHemische Industrie) scale. The Magarey generic fungal model was applied for the identification of the main meteorological suitable periods for infection within the susceptible phenological stages of flowering and ripening of berries. Our results showed that climatic conditions favoured fungal development during flowering, although a higher incidence of B. cinerea infection risk-periods occurred during the prior-to-harvest stage of ripening of berries, the most susceptible phenological stage to B. cinerea infection obtained by the proposed methodology. This approach enables more precise targeting in pesticide spraying and reduction in pesticide application from 4–5 to 2–3 times per year at our commercial study. It also illustrates the real-world benefits of integrated disease risk modelling.
Exploring species diversity of lissorchiid trematodes (Digenea: Lissorchiidae) associated with the gravel snail,Lithoglyphus naticoides, in European freshwaters
Authors: Petkevičiūtė R. , Stanevičiūtė G. , Stunžėnas V.
Published: May 2020

Comparative analysis using complete ITS2 and partial 28S rDNA sequence data revealed that cercariaeum developing in rediae inLithoglyphus naticoidesrepresent two different lissorchiid species. One morphotype of cercariaeum is conspecific with adultPalaeorchis incognitusfrom European roach,Rutilus rutilus. The other cercariaeum is attributable to the genusAsymphylodora, but the species identity is not yet determined. We also generate the first rDNA sequences forAsymphylodora progeneticabased on new collections fromBithynia tentaculatafrom Lithuania. Phylogenetic analyses of the newly generated sequences, together with information for other lissorchiids available on GenBank, showed that all representatives of Lissorchiidae form a strongly supported clade. Three monophyletic lineages,Asymphylodora,PalaeorchisandLissorchis, were recognized at the generic level. Karyological analysis of the chromosome set of larvalP. incognitusrevealed a diploid number of 2n= 20. Its karyotype with subtelocentric chromosomes prevailing can be regarded as comparatively ‘primitive’, which is consistent with the basal position ofP. incognitusin the 28S tree relative to the representatives of the genusAsymphylodora. The present study adds significant new information for establishing species-specific markers for the confident characterization of different developmental stages of lissorchiid species and clarification of their life cycles.
New records of Ericthonius didymus Krapp-Schickel, 2013 (Crustacea: Amphipoda: Ischyroceridae) in European waters with a focus in Arcachon Bay, France and key to Ericthonius species
Authors: Gouillieux Benoit , Ariyama Hiroyuki , Costa Ana Cristina , Daffe Guillemine , Marchini Agnese , Micael Joana , Ulman Aylin
Published: May 2020

The amphipod Ericthonius didymus Krapp-Schickel, 2013 is reported for the first time outside its type locality in European waters, from the French Atlantic coast and from the Azores, Portugal. In particular, a large population was recorded in Arcachon, France. Molecular analyses were made to confirm the validity of three closely related Ericthonius species: E. didymus, E. convexus and E. pugnax. An identification key is provided, distinguishing Ericthonius species into three groups, based on the presence or absence of stridulating ridges on coxa 2 and posterodistal lobe on basis of male pereopod 5.
Evaluation of the impact of patient involvement in health technology assessments: A scoping review
Authors: Mason Robert J. , Searle Karlee M. , Bombard Yvonne , Rahmadian Amanda , Chambers Alexandra , Mai Helen , Morrison Marjorie , Chan Kelvin K. W. , Jerzak Katarzyna J.
Published: May 2020

Objectives

While involving patients in health technology assessment (HTA) has become increasingly common and important around the world, little is known about the optimal methods of evaluating patients’ involvement (PI) in HTA. This scoping review was undertaken to provide an overview of currently available methods for the evaluation of PI, specifically the impact of PI on HTA recommendations.

Methods

A literature search was conducted using nine databases as well as a grey literature search of the websites of 26 organizations related to the conduct, practice or research of HTA to identify articles, reports and abstracts related to the evaluation of PI impact in HTA.

Results

We identified 1,248 unique citations, six of which met our eligibility criteria. These six records (five articles, and one report) were all published after 2012. Four assessed the impact of patient experience submissions on final HTA recommendations; one evaluated the impact of direct involvement on HTA committees, and one assessed impact of multiple forms of involvement. Methods of evaluation included quantitative analyses of reimbursement decisions, qualitative interviews with those directly involved in an assessment, surveys of patient groups and committee members, and the review of HTA reports.

Conclusions

Quantitative evaluation of PI based on associations with funding decisions may not be feasible or fully capture the relevant impact of PI in the assessment of health technologies. Rather, a combination of both qualitative and quantitative strategies may allow for the most comprehensive assessment of the impact of PI on HTA recommendations when possible.
VOLATILITY ANALYSIS OF REGIME-SWITCHING MODELS
Authors: Liu Yue , Xie Zhuyun , Yao Jingjing , Li Kaodui
Published: May 2020

This paper investigates the volatility in regime-switching models formulated based on the geometric Brownian motion with its drift and volatility factors randomized with Markov chains. By developing explicit formulas about occupation time of Markov chains, we analysis the difference between global volatility of this model and the volatility caused by Brownian randomness, in order to measure the volatility caused by regime-switching after justifying its existence. Utilizing this structure of volatility, we optimize the methods of volatility parameters estimation.
Prognostic roles of time to positivity of blood cultures in patients with Escherichia coli bacteremia
Authors: Chen Yufang , Huang Xun , Wu Anhua , Lin Xuan , Zhou Pengcheng , Liu Yao , Wu Yayun , Fu Chenchao , Dou Qingya , Jiang Huaye
Published: May 2020

The time to positivity (TTP) of blood cultures has been considered a predictor of clinical outcomes for bacteremia. This retrospective study aimed to determine the clinical value of TTP for the prognostic assessment of patients with Escherichia coli bacteremia. A total of 167 adult patients with E.coli bacteremia identified over a 22-month period in a 3500-bed university teaching hospital in China were studied. The standard cut-off TTP was 11 h in the patient cohort. The septic shock occurred in 27.9% of patients with early TTP (⩽11 h) and in 7.1% of those with a prolonged TTP (>11 h) (P = 0.003). The mortality rate was significantly higher for patients in the early than in the late group (17.7% vs. 4.0%, P < 0.001). Multivariate analysis showed that an early TTP (OR 4.50, 95% CI 1.70–11.93), intensive care unit admission (OR 8.39, 95% CI 2.01–35.14) and neutropenia (OR 4.20, 95% CI 1.55–11.40) were independently associated with septic shock. Likewise, the independent risk factors for mortality of patients were an early TTP (OR 3.80, 95% CI 1.04–12.90), intensive care unit admission (OR 6.45; 95% CI 1.14–36.53), a Pittsburgh bacteremia score ⩾2 (OR 4.34, 95% CI 1.22–15.47) and a Charlson Comorbidity Index ⩾3 (OR 11.29, 95% CI 2.81–45.39). Overall, a TTP for blood cultures within 11 h appears to be associated with worse outcomes for patients with E.coli bacteremia.
Interleukin-1β and TNF-α systems in ovarian follicles and their roles during follicular development, oocyte maturation and ovulation
Authors: Silva José R.V. , Lima Francisco E.O. , Souza Ana L.P. , Silva Anderson W.B.
Published: May 2020

Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are cytokines that are involved in the development, proliferation and apoptosis of ovarian follicular cells in domestic mammals. The expression of these cytokines in various follicular compartments, depending on the stage of follicle development, demonstrates their involvement in the control of primordial follicle growth up to the preovulatory stage. The mechanism of action of these factors depends on the presence of their receptors that transduce their biological actions. This review shows the expression sites of TNF-α, IL-1β and their receptors in ovarian follicles, and discusses the mechanism of action of these cytokines during follicle development, oocyte maturation and ovulation in domestic animals.
Transcatheter radiofrequency ablation using near-zero fluoroscopy in children with fascicular ventricular tachycardia: a single-centre experience
Authors: Balli Sevket , Kucuk Mehmet , Epçaçan Serdar
Published: May 2020

Objective:

Fascicular tachycardia is a common form of sustained idiopathic left ventricular tachycardia. This study aimed to achieve successful results with catheter ablation procedures performed through three-dimensional electroanatomic mapping using near-zero fluoroscopy in fascicular tachycardia patients.

Methods and results:

In this study, we included 33 consecutive children with fascicular tachycardia, for whom we performed a transcatheter radiofrequency ablation procedure using the EnSite® system. Activation mapping was performed in all patients during tachycardia, and the earliest presystolic purkinje potentials were the target site for radiofrequency lesions.

Results:

Twenty-five patients were male, and eight were female. The mean weight of the patients was 39.6 ± 10.4 kg, and the mean age was 13.6 ± 2.5 years. The mean procedure time was 121.3 ± 44.3 minutes. The mean follow-up period was 18.4 ± 6.5 months. No fluoroscopy was needed in 30 patients. The mean fluoroscopy time in the remaining patients was 166.6 ± 80 seconds. All of the patients had left posterior fascicular tachycardia except for one who had left anterior fascicular tachycardia. The acute success rate was perfect (100%). No patients developed left bundle branch block or complete atrioventricular block. Recurrence developed in one patient.

Conclusion:

We suggest that radiofrequency ablations via an electroanatomic mapping system are quite safe and effective, with high success rates in paediatric patients with fascicular tachycardia. This method has the advantage of avoiding ionising radiation exposure for both the patient and operator, thus reducing the lifetime risk of malignancy in the paediatric population.
A systematic review of interventions to improve the dietary intake, physical activity and weight status of children attending family day care services
Authors: Yoong Sze Lin , Lum Melanie , Jones Jannah , Kerr Erin , Falkiner Maryann , Delaney Tessa , McCrabb Sam , Chai Li Kheng , Seward Kirsty , Grady Alice
Published: May 2020

Objective:

To (i) identify and synthesise findings from interventions to improve the dietary intake, physical activity and weight status of children aged 0–6 years attending family day care services; and (ii) assess the impact of interventions on family day care environments, intervention cost and adverse outcomes.

Design:

Medline in Process, PsycINFO, ERIC, Embase, CINAHL, CENTRAL and Scopus databases were searched in March 2019. Studies were included if they (i) evaluated an intervention to improve the diet, physical activity and/or weight of children aged 0–6 years; (ii) were delivered in family day care services; (iii) targeted child diet, physical activity and/or weight; and (iv) used a parallel control group design. Screening was undertaken by two reviewers with disagreements resolved by a third reviewer.

Setting:

Family day care services, also known as family childcare homes.

Participants:

Children aged 0–6 years attending family day care services.

Results:

In total, 8977 titles were retrieved, and 199 full-texts reviewed. No studies met the inclusion criteria for the primary outcome; however, two studies reporting on the secondary outcome of family day care environments were included. The 4-year community-wide obesity prevention programme and the 12-month train-the-trainer programme both reported statistically significant improvements in the healthy eating and physical activity environments of family day care, compared to cross-sectional state-average control groups.

Conclusions:

Findings highlight few existing interventions in family day care services and a need for high-quality controlled trials to identify effective interventions to improve children’s diet, activity and weight in this setting.
Constraints on the Timing of Explosive Volcanism at Aso and Aira Calderas (Japan) Between 50 and 30 ka: New Insights From the Lake Suigetsu Sedimentary Record (SG14 Core)
Authors: McLean D. , Albert P. G. , Suzuki T. , Nakagawa T. , Kimura J.‐I. , Chang Q. , Miyabuchi Y. , Manning C. J. , MacLeod A. , Blockley S. P. E. , Staff R. A. , Yamada K. , Kitaba I. , Yamasaki A. , Haraguchi T. , Kitagawa J. ,   SG14 Project Members, Smith V. C.
Published: May 2020

Volcanoes in the East Asian/Pacific region have been the source of some of the largest magnitude eruptions during the Late Quaternary, and accurately evaluating their eruptive histories is essential for hazard assessments. To overcome difficulties in resolving and precisely dating eruptions in the near‐source realm, the high‐resolution (varved) sediments of Lake Suigetsu (central Honshu, Japan) were examined for the presence of non‐visible (cryptotephra) layers from 50 ka up until the 30 ka Aira‐Tanzawa (AT) caldera‐forming event of Aira volcano. Cryptotephra layers are four times more frequently preserved than visible markers in the Suigetsu sediments, meaning that this archive provides a unique and unprecedented record of eruptions that were dispersed over the densely populated regions of central Honshu. Major and trace element volcanic glass chemistry is used to fingerprint the ash layers and pinpoint their volcanic origin. Tephras are found throughout the investigated sediments, but the highest abundance of ash fall events are recorded between 39 and 30 ka, capturing a period of intense volcanism at calderas on Kyushu Island (Japan). The augmented Suigetsu tephrostratigraphy records at least seven eruptions from Aso caldera (southern Kyushu) that post‐date the widespread ACP‐4 Plinian eruption (ca. 50 ka) and four explosive events from Aira (central Kyushu) that occurred leading up to the catastrophic caldera‐forming AT eruption (ca. 30 ka).
Rapid Viscoelastic Deformation Slows Marine Ice Sheet Instability at Pine Island Glacier
Authors: Kachuck S. B. , Martin D. F. , Bassis J. N. , Price S. F.
Published: May 2020

The ice sheets of the Amundsen Sea Embayment (ASE) are vulnerable to the marine ice sheet instability (MISI), which could cause irreversible collapse and raise sea levels by over a meter. The uncertain timing and scale of this collapse depend on the complex interaction between ice, ocean, and bedrock dynamics. The mantle beneath the ASE is likely less viscous (∼1018 Pa s) than the Earth's average mantle (∼1021 Pa s). Here we show that an effective equilibrium between Pine Island Glacier's retreat and the response of a weak viscoelastic mantle can reduce ice mass lost by almost 30% over 150 years. Other components of solid Earth response—purely elastic deformations and geoid perturbations—provide less stability than the viscoelastic response alone. Uncertainties in mantle rheology, topography, and basal melt affect how much stability we expect, if any. Our study indicates the importance of considering viscoelastic uplift during the rapid retreat associated with MISI.
Crossbreeding CMIP6 Earth System Models With an Emulator for Regionally Optimized Land Temperature Projections
Authors: Beusch Lea , Gudmundsson Lukas , Seneviratne Sonia I.
Published: May 2020

The newest generation of the Coupled Model Intercomparison Project (CMIP6) exhibits a larger spread in temperature projections at the end of the 21st century than the previous generation. Here, a modular Earth System Model emulator is used to evaluate the realism of the warming signal in CMIP6 models on both global and regional scales, by comparing their global trends and regional response parameters to observations. Subsequently, the emulator is employed to derive large “crossbred” multimodel initial‐condition ensembles of regionally optimized land temperature projections by combining observationally constrained global mean temperature trend trajectories with observationally constrained local parameters. In the optimized ensembles, the warmest temperature projections are generally reduced and for the coolest projections both higher and lower values are found, depending on the region. The median shows less changes in large parts of the globe. These regional differences highlight the importance of a geographically explicit evaluation of Earth System Model projections.
Small‐Scale Topographic Form Stress and Local Dynamics of the Southern Ocean
Authors: Zhang Xihan , Nikurashin Maxim
Published: May 2020

The contribution of small‐scale abyssal hill topography to the topographic form stress and local dynamics of the Southern Ocean is investigated using a high‐resolution model of the sector of the Southern Ocean south of Tasmania and New Zealand. The results of two simulations, with and without small, O(1–100 km), scale topography, confirm that the effects of small‐scale topography are exerted through the generation of strong topographic form stress leading to transient eddy dissipation and changes in flow meanders. Small‐scale topographic form stress is comparable in magnitude to that generated by large‐scale topography, but with a pairwise distribution of positive and negative stress values upstream and downstream of the Macquarie Ridge, consistent with the meandering of the flow. In the experiment without small‐scale topography, the bottom mean flow speed increases, while the surface mean speed slightly decreases, making the mean flow more barotropic. Eddy kinetic energy also greatly enhances throughout the water column after removing small‐scale topography. Our results suggest that small‐scale topography has strong impact on transient eddies and plays an important role for setting the vertical structure of the flow and the equilibration and position of flow meanders.