bluuebunny/crossref_metadata_2025 huggingface dataset:
Cardiorenal Systems Modeling: Left Ventricular Hypertrophy and Differential Effects of Antihypertensive Therapies on Hypertrophy Regression
Authors: Hallow K. Melissa , Van Brackle Charles H. , Anjum Sommer , Ermakov Sergey
Published: June 2021

Cardiac and renal function are inextricably connected through both hemodynamic and neurohormonal mechanisms, and the interaction between these organ systems plays an important role in adaptive and pathophysiologic remodeling of the heart, as well as in the response to renally acting therapies. Insufficient understanding of the integrative function or dysfunction of these physiological systems has led to many examples of unexpected or incompletely understood clinical trial results. Mathematical models of heart and kidney physiology have long been used to better understand the function of these organs, but an integrated model of renal function and cardiac function and cardiac remodeling has not yet been published. Here we describe an integrated cardiorenal model that couples existing cardiac and renal models, and expands them to simulate cardiac remodeling in response to pressure and volume overload, as well as hypertrophy regression in response to angiotensin receptor blockers and beta-blockers. The model is able to reproduce different patterns of hypertrophy in response to pressure and volume overload. We show that increases in myocyte diameter are adaptive in pressure overload not only because it normalizes wall shear stress, as others have shown before, but also because it limits excess volume accumulation and further elevation of cardiac stresses by maintaining cardiac output and renal sodium and water balance. The model also reproduces the clinically observed larger LV mass reduction with angiotensin receptor blockers than with beta blockers. We further provide a mechanistic explanation for this difference by showing that heart rate lowering with beta blockers limits the reduction in peak systolic wall stress (a key signal for myocyte hypertrophy) relative to ARBs.
Cyberbullying Involvement, Resilient Coping, and Loneliness of Adolescents During Covid-19 in Rural China
Authors: Han Ziqiang , Wang Ziyi , Li Yuhuan
Published: June 2021

Cyberbullying involvement can lead to internal health issues, especially mental health problems. Different coping strategies may reduce or enhance the strengths between cyberbullying experience and mental health problems. In this study, we examined the correlations between cyberbullying involvement and loneliness among a group of children and adolescents during the Covid-19 pandemic in China, focusing on investigating the protecting effect of the resilient coping strategy. The results demonstrated that 86.68% of the students were not involved in cyberbullying activities, 8.19% were victims only, 1.89% was perpetrators only, and 3.24% were both victims and perpetrators. Compared with the non-involved, the victims-only group had a significantly higher degree of reported loneliness and a lower score of resilient coping, while the differences of the other groups were not significant. Resilient coping strategy can significantly reduce loneliness and play a mediating role between cyberbullying victimization and loneliness, but such mitigating effect was relatively weak. Besides, peer relations were the primary protective factors, and age was the primary risk factor of loneliness among the controlled variables. This study can enrich current knowledge of cyberbullying involvement and the psychological health among children and adolescents, especially in the context of the pandemic.
Catharsis Through Cinema: An Italian Qualitative Study on Watching Tragedies to Mitigate the Fear of COVID-19
Authors: Testoni Ines , Rossi Emil , Pompele Sara , Malaguti Ilaria , Orkibi Hod
Published: June 2021

Background: Among different ways of coping with the unsettling situation of the COVID-19 pandemic, a very peculiar one has been identified: a more frequent request, by the general population, of movies or TV series related to the very theme of viruses, contagions, and epidemics.

Objectives: The aim of the present study was to explore this peculiar phenomenon, in order to identify people's emotions and cognitions during and after the process, and to better understand the possible psychological function cinema can have during moments of intense and generalized crisis like the present COVID-19 pandemic.

Participants: Fifteen Italian adults took part in the study – eight women and seven men (average age = 30 years, SD = 10.54). Participants were recruited through social media via a specific announcement, and subsequently, through a “snowball sampling.”

Method: For the present study a qualitative approach was adopted, and more specifically, the principles of Interpretative Phenomenological Analysis (IPA) have been followed. Semi-structured interviews were conducted by telephone or through online meeting platforms (Zoom or Skype). The written texts obtained from the transcription of each interview were analyzed using thematic analysis with the support of the software Atlas.ti, in order to highlight their fundamental contents.

Results: From the data analysis, four main areas of thematic prevalence emerged, which reflected the participants' condition during the pandemic that actually led them to watch epidemic-themed movies or TV series: the need to document themselves concerning the theme of epidemics, the need to exorcize contagion anxiety, the desire to find a character with which they could identify, and the casual condition of finding a peculiar movie related to the theme of pandemics and being interested in it because of the striking resemblance with real life.

Conclusions: The findings suggest that cinema could indeed represent a powerful tool to help people face the intense uncertainty of the new COVID-19 epidemic, since it allows spectators to both obtain more insight into the current situation, and to project their fears and uncertainties into the movie or TV series, thus reaching a sort of cathartic liberation that offers them hope toward the future.
Creating a Social Learning Environment for and by Older Adults in the Use and Adoption of Smartphone Technology to Age in Place
Authors: Haan Marjolein den , Brankaert Rens , Kenning Gail , Lu Yuan
Published: June 2021

Smartphone technologies can support older adults in their daily lives as they age in place at home. However, they may struggle to use these technologies which impacts acceptance, adoption, and sustainable use. Peer to peer community learning has the potential to support older adults to learn using (smartphone) technologies. This paper studies such a learning community approach and how it can support older adults to learn using and adopt the smartphone application GoLivePhone. This technology assists older adults in their daily living by supporting them through fall detection and activity tracking. In particular, the interface of this application can evolve and adapt as older adults become more knowledgeable during the use process or as their abilities change. This paper shows a field study with seven older adults learning and using the GoLivePhone technology through a living lab approach. These older adults participated in this research in a technology learning community that was set-up for research purposes. For this we used ordinary Samsung A3 smartphones with the simplified GoLivePhone software, particularly designed for older adults. At the end of the learning class we conducted an additional focus group to both explore factors facilitating older adults to learn using this technology and to identify their main personal drivers and motivators to start and adopt this technology. We collected qualitative data via open questions and audio recording during the focus group. This collected data was subject to a thematic analysis, coding was primarily performed by the first author, and reviewed by the other authors. We provide insights into how peer to peer community learning can contribute, and found both super-users and recall tools to be helpful to support sustainable use of smartphone technology to support older adults to age in place.
Digital Overuse and Addictive Traits and Their Relationship With Mental Well-Being and Socio-Demographic Factors: A National Population Survey for Wales
Authors: Bellis Mark A. , Sharp Catherine A. , Hughes Karen , Davies Alisha R.
Published: June 2021

Introduction: Population health concerns have been raised about negative impacts from overuse of digital technologies. We examine patterns of online activity predictive of Digital Overuse and Addictive Traits (DOAT). We explore associations between DOAT and mental well-being and analyse how both relate to self-reported changes in self-esteem, perceived isolation, and anxiety about health when individuals use the internet for health purposes.

Methods: A cross-sectional nationally representative household survey of adults using stratified random sampling (compliance 75.4%, n = 1,252). DOAT was measured using self-reported questions adapted from a social media addiction scale (failure to cut down use, restlessness when not using, and impact on job/studies and home/social life in the last year), combined into a single DOAT score. Higher DOAT score was defined as >1 standard deviation above population mean. The Short Warwick-Edinburgh Mental Well-being Scale was used to measure mental well-being. Analyses were limited to those with internet access (n = 1,003).

Results: Negative impacts of digital technology use on work and home/social lives were reported by 7.4% of respondents. 21.2% had tried but failed to cut down use in the past year. Higher DOAT was associated with higher social media and internet use but also independently associated with greater risks of low mental well-being. Higher DOAT was associated with both improvement and worsening of self-esteem, perceived isolation and anxiety about health when using the internet for health reasons, with no change in these outcomes most likely in those with lower DOAT. Lower mental well-being was associated with a similar bi-directional impact on perceived isolation and was also associated with worsening self-esteem.

Conclusions: Substantial proportions of individuals report negative impacts on home, social and working lives from digital technology use, with many trying but failing to cut down use. Individuals with higher DOAT may experience improvements or worsening in self-esteem and other measures of mental well-being when using the internet for health purposes. From a public health perspective, a greater understanding of risk factors for digital overuse, its impacts on well-being and how to reasonably limit use of technology are critical for a successful digital revolution.
A Citywide ‘Virus Testing': Chinese Government's Response to Preventing and Controlling the Second Outbreak of SARS-CoV-2
Authors: Zhou Liting , Seesaghur Hans Nibshan , Akhtar Nadeem , Boolakee Jason , Pratt Cornelius B.
Published: June 2021

Containing the spread of SARS-CoV-2 is a daunting challenge globally. China, as well as a handful of other countries, has, for the most part, contained it by implementing strict policies. Wuhan's citywide virus-testing program presents a way forward in preventing and controlling the uncertainty, anxiety, instability and complexity it faces over the outbreak of SARS-CoV-2. Inarguably, the health crisis requires time-tested strategies and tactics for coordinating governments' and social entities' response to the health crisis, with a goal toward having and ensuring sustained effectiveness. Because of a possible recurrence of SARS-CoV-2 in Wuhan, the Prevention and Control Headquarters of Wuhan on COVID-19 launched a massive virus testing of Wuhan's 11 million residents; it was completed within 10 days. In light of this unprecedented mass testing, this study applies the situational crisis communication theory to analyze this massive virus-testing process and the mechanisms involved to contain SARS-CoV-2 in Wuhan. While many countries still have partial lockdowns, the second outbreak in Wuhan was an indication of what awaited all SARS-CoV-2-stricken countries post-lockdowns and after community restrictions had been lifted. Therefore, the recently implemented Wuhan control mechanism (in cities, districts and townships) may become a hortatory guide to other world regions as they contend with and consider appropriate measures to control the spread of SARS-CoV-2 and to ensure public safety.
Helping Mothers Survive: Program Evaluation and Early Outcomes of Maternal Care Training in the Dominican Republic
Authors: Smith Meghan , Leader Alexandra , Roa Wanny , Jaramillo Ericka , Lazala Davina , Flores Jose , Cadet Claudia , Vazifedan Turaj , Bentley Suzanne , Jensen Lloyd
Published: June 2021

Introduction: In 2017, approximately 295,000 women died during and immediately following pregnancy and childbirth worldwide, with 94% of these deaths occurring in low-resource settings. The Dominican Republic (DR) exhibits one of the highest maternal mortality ratios in the region of Latin America and the Caribbean despite the fact that 99% of registered births in the country are reportedly attended by a skilled birth attendant. This paradox implies that programs to support healthcare worker knowledge and skills improvement are vital to improving maternal health outcomes in the DR. Helping Mothers Survive (HMS) is a provider training program developed by Jhpiego and global partners. The goal of HMS is to combat maternal mortality by contributing to quality improvement efforts that reinforce maternal health skills of local healthcare workers.

Methods: An international, multisectoral group of stakeholders collaborated in the implementation of two HMS curricula, Bleeding After Birth (BAB) and pre-eclampsia & eclampsia (PE&E). Demographic information as well as pre- and post-training knowledge scores were recorded for each participant. Knowledge score improvement was assessed in order to support effectiveness of the program on knowledge acquisition of healthcare workers.

Results: Three hundred and twenty healthcare workers participated in the HMS training workshops between October 2016–August 2020. Of the 320 participants, 132 were trained as master trainers. The majority of participants identified as attending physicians, followed by residents/interns, nurses, students, and “other.” A significant improvement in knowledge scores was observed for both the BAB and PE&E curricula, with a 21.24 and 30.25% change in average score (pre- to post-test), respectively. In response to COVID-19 pandemic restrictions, flexibility of the local team led to a PE&E virtual training pilot workshop in August 2020.

Discussion/Conclusions: Simulation-based training improved the knowledge levels of healthcare workers for both HMS curricula. These results suggest that simulation-based workshops have an impact on knowledge acquisition and skills of healthcare workers immediately following training. For the PE&E curriculum, no significant difference in knowledge acquisition was observed between in-person and virtual training sessions. The ongoing pandemic poses challenges to program implementation; however, these preliminary results provide evidence that conducting virtual workshops may be a viable alternative to in-person training.
Data Sharing in Southeast Asia During the First Wave of the COVID-19 Pandemic
Authors: Amit Arianna Maever L. , Pepito Veincent Christian F. , Gutierrez Bernardo , Rawson Thomas
Published: June 2021

Background: When a new pathogen emerges, consistent case reporting is critical for public health surveillance. Tracking cases geographically and over time is key for understanding the spread of an infectious disease and effectively designing interventions to contain and mitigate an epidemic. In this paper we describe the reporting systems on COVID-19 in Southeast Asia during the first wave in 2020, and highlight the impact of specific reporting methods.

Methods: We reviewed key epidemiological variables from various sources including a regionally comprehensive dataset, national trackers, dashboards, and case bulletins for 11 countries during the first wave of the epidemic in Southeast Asia. We recorded timelines of shifts in epidemiological reporting systems and described the differences in how epidemiological data are reported across countries and timepoints.

Results: Our findings suggest that countries in Southeast Asia generally reported precise and detailed epidemiological data during the first wave of the pandemic. Changes in reporting rarely occurred for demographic data, while reporting shifts for geographic and temporal data were frequent. Most countries provided COVID-19 individual-level data daily using HTML and PDF, necessitating scraping and extraction before data could be used in analyses.

Conclusion: Our study highlights the importance of more nuanced analyses of COVID-19 epidemiological data within and across countries because of the frequent shifts in reporting. As governments continue to respond to impacts on health and the economy, data sharing also needs to be prioritised given its foundational role in policymaking, and in the implementation and evaluation of interventions.
Hybrid Parallel Compliance Allows Robots to Operate With Sensorimotor Delays and Low Control Frequencies
Authors: Ashtiani Milad Shafiee , Aghamaleki Sarvestani Alborz , Badri-Spröwitz Alexander
Published: June 2021

Animals locomote robustly and agile, albeit significant sensorimotor delays of their nervous system and the harsh loading conditions resulting from repeated, high-frequent impacts. The engineered sensorimotor control in legged robots is implemented with high control frequencies, often in the kilohertz range. Consequently, robot sensors and actuators can be polled within a few milliseconds. However, especially at harsh impacts with unknown touch-down timing, controllers of legged robots can become unstable, while animals are seemingly not affected. We examine this discrepancy and suggest and implement a hybrid system consisting of a parallel compliant leg joint with varying amounts of passive stiffness and a virtual leg length controller. We present systematic experiments both in computer simulation and robot hardware. Our system shows previously unseen robustness, in the presence of sensorimotor delays up to 60 ms, or control frequencies as low as 20 Hz, for a drop landing task from 1.3 leg lengths high and with a compliance ratio (fraction of physical stiffness of the sum of virtual and physical stiffness) of 0.7. In computer simulations, we report successful drop-landings from 3.8 leg lengths (1.2 m) for a 2 kg quadruped robot with 100 Hz control frequency and a sensorimotor delay of 35 ms.
Robot Care Ethics Between Autonomy and Vulnerability: Coupling Principles and Practices in Autonomous Systems for Care
Authors: Pirni Alberto , Balistreri Maurizio , Capasso Marianna , Umbrello Steven , Merenda Federica
Published: June 2021

Technological developments involving robotics and artificial intelligence devices are being employed evermore in elderly care and the healthcare sector more generally, raising ethical issues and practical questions warranting closer considerations of what we mean by “care” and, subsequently, how to design such software coherently with the chosen definition. This paper starts by critically examining the existing approaches to the ethical design of care robots provided by Aimee van Wynsberghe, who relies on the work on the ethics of care by Joan Tronto. In doing so, it suggests an alternative to their non-principled approach, an alternative suited to tackling some of the issues raised by Tronto and van Wynsberghe, while allowing for the inclusion of two orientative principles. Our proposal centres on the principles of autonomy and vulnerability, whose joint adoption we deem able to constitute an original revision of a bottom-up approach in care ethics. Conclusively, the ethical framework introduced here integrates more traditional approaches in care ethics in view of enhancing the debate regarding the ethical design of care robots under a new lens.
Educational Robotics and Robot Creativity: An Interdisciplinary Dialogue
Authors: Gubenko Alla , Kirsch Christiane , Smilek Jan Nicola , Lubart Todd , Houssemand Claude
Published: June 2021

There is a growing literature concerning robotics and creativity. Although some authors claim that robotics in classrooms may be a promising new tool to address the creativity crisis in school, we often face a lack of theoretical development of the concept of creativity and the mechanisms involved. In this article, we will first provide an overview of existing research using educational robotics to foster creativity. We show that in this line of work the exact mechanisms promoted by robotics activities are rarely discussed. We use a confluence model of creativity to account for the positive effect of designing and coding robots on students' creative output. We focus on the cognitive components of the process of constructing and programming robots within the context of existing models of creative cognition. We address as well the question of the role of meta-reasoning and emergent strategies in the creative process. Then, in the second part of the article, we discuss how the notion of creativity applies to robots themselves in terms of the creative processes that can be embodied in these artificial agents. Ultimately, we argue that considering how robots and humans deal with novelty and solve open-ended tasks could help us to understand better some aspects of the essence of creativity.
A Study on the Hysteresis Effect and Spectral Evolution in the Mini-Outbursts of Black Hole X-Ray Binary XTE J1550-564
Authors: Dong Ai-Jun , Liu Chang , Ge Kang , Liu Xiang , Zhi Qi-Jun , You Zi-Yi
Published: June 2021

One normal outburst and three mini-outbursts have been detected by Rossi X-ray Timing Explorer satellite after 2000 in the well-known black hole X-ray binary XTE J1550-564. In this work, we explore the hysteresis effect of the four outbursts, which is a phenomenon that a similar spectral state transition occurs at different luminosity in an outburst of black hole X-ray binary. A q-like track was found in the hardness-intensity diagram of the normal outburst in 2000 but not in the three mini-outbursts that only occur in the Low/Hard state. The results demonstrate that the hysteresis effect is not apparent in the three mini-outbursts and the X-ray spectra are harder than that of the normal outburst at the same photon count rate. Furthermore, the results of the correlation analysis show that the Γ − F2−10keV correlation of mini-outburst maintain negative in the Low/Hard state with the harder spectra than that of the normal outburst at the same X-ray flux. The X-ray spectral evolution can be well-explained by the state-transition model from the Shakura–Sunyaev disk to the advection-dominated accretion flow, which implies that the three mini-outbursts of XTE J1550-564 might originate from a smaller discrete accretion event.
Assessing Smallholder Farmer's Participation in the Wheat Value Chain in North-West Mt. Kenya
Authors: Mwangi Veronica , Owuor Samuel , Kiteme Boniface , Giger Markus
Published: June 2021

Wheat is the second most important carbohydrate staple in Kenya and is produced by both smallholder and large-scale farmers. Smallholders are the majority, but produce <20% of the total national production. Compared to large-scale farmers, they have been considered to be less efficient producers and thus fail to benefit fully from their participation in the wheat value chain. This study aims at establishing the value accruing to smallholder and large-scale farmers participating in wheat production in north-west Mt. Kenya. For comparative purposes and to explore the potential of smallholder farmers, a sample of 58 smallholder and seven large-scale farmers was selected for the study. We use budget analysis to determine the gross output, cost of production and gross margins attained by the smallholder and large-scale farmers. Further, an analysis of constraints to productivity is done to establish the factors hindering farmers from reaching high yield potential. Results of the study show that smallholder farmers obtain lower yields, have higher costs of production per bag of wheat and lower gross margins compared to large-scale farmers. High cost of inputs, low market prices, low bargaining power, high cost of machinery services, diseases and weeds were among factors excluding smallholder farmers from benefitting from their participation in the value chain. However, results also show that smallholder farmers can produce wheat profitably albeit with necessary support. Thus, interventions should consider these constraints and aim at improving smallholder farmers' horizontal and vertical integration in the value chain. We recommend continued investment in research and development on wheat, adoption of climate resilient agricultural practices, improvements in the fertilizer subsidy programme and creating or increasing participation in producer groups that will provide possibilities of increased bargaining power and reduction of costs through improved access to machinery services, markets and credit.
Genetic Parameters for First Lactation and Lifetime Traits of Nili-Ravi Buffaloes
Authors: Tamboli Pooja , Bharadwaj Anurag , Chaurasiya Amit , Bangar Yogesh Chandrakant , Jerome Andonissamy
Published: June 2021

The data on first lactation and lifetime performance records of 501 Nili-Ravi were collected for a period from 1983 to 2017 (35 years) maintained at ICAR-Central Institute for Research on Buffaloes, Sub-Campus, Nabha, Punjab. The data were analyzed to calculate heritability, genetic and phenotypic correlation for first lactation traits, viz., Age at First Calving (AFC), First Lactation Total Milk Yield (FLTMY), First Lactation Standard (305 days or less) Milk Yield (FLSMY), First Peak Milk Yield (FPY), First Lactation Length (FLL), First Dry Period (FDP), First Service Period (FSP) and First Calving Interval (FCI), Herd Life (HL), Productive Life (PL), Productive Days (PD), Unproductive Days (UD), Breeding Efficiency (BE), Total Lifetime Milk Yield (Total LTMY), Standard Lifetime Milk Yield (Standard LTMY), Milk Yield Per Day of Productive Life (MY/PL), Milk Yield Per Day of Productive Days (MY/PD), and Milk Yield Per Day of Herd Life (MY/HL). For estimation of variance component and heritability separately for each trait, the uni-trait animal model was equipped, whereas to estimate genetic and phenotypic correlations between traits, bi-trait animal models were fitted. The estimates of heritability for production and reproduction traits of Nili-Ravi were medium, i.e., 0.365 ± 0.087, 0.353 ± 0.071, 0.318 ± 0.082, 0.354 ± 0.076, and 0.362 ± 0.086 for FLSMY, FDP, FSP, FCI, and AFC, respectively. The estimates of heritability were low, i.e., 0.062 ± 0.088, 0.123 ± 0.090, 0.158 ± 0.090, 0.155 ± 0.091, and 0.129 ± 0.091 for HL, PL, PD, Total LTMY, and Standard LTMY and high, i.e., 0.669 ± 0.096 for BE. Genetic correlation for FLTMY was high with FLL (0.710 ± 0.103), and genetic correlation of FLTMY was high and positive with HL, Total LTMY, MY/PL, and MY/PD while low and positive with PL. Genetic correlation of AFC was low and negative with PL, PD, UD, BE, Total LTMY, Standard LTMY, MY/PL, and MY/PD and negative with MY/HL. Significant positive phenotypic association of FPY was seen with FLTMY, FLSMY, FLL, AFC, HL, Total LTMY, and Standard LTMY. Higher heritability of first lactation traits especially FPY suggests sufficient additive genetic variability, which can be exploited under selection and breeding policy in order to improve overall performance of Nili-Ravi buffaloes.
Identification of Differentially Expressed Non-coding RNA Networks With Potential Immunoregulatory Roles During Salmonella Enteritidis Infection in Ducks
Authors: Zhang Yu , Dong Xiaoqian , Hou Lie , Cao Zhengfeng , Zhu Guoqiang , Vongsangnak Wanwipa , Xu Qi , Chen Guohong
Published: June 2021

Salmonella enterica serovar Enteritidis (S. Enteritidis) is a pathogen that can colonize the preovulatory follicles of poultry, thereby causing both reduced egg production and an elevated risk of foodborne salmonellosis in humans. Although a few studies have revealed S. Enteritidis preferentially invades the granulosa cell layer within these follicles, it can readily persist and proliferate through mechanisms that are not well-understood. In this study, we characterized competing endogenous RNA (ceRNA) regulatory networks within duck granulosa cells following time-course of S. Enteritidis challenge. The 8108 long non-coding RNAs (lncRNAs), 1545 circular RNAs (circRNAs), 542 microRNAs (miRNAs), and 4137 mRNAs (fold change ≥2; P < 0.01) were differentially expressed during S. Enteritidis challenge. Also, eight mRNAs, eight lncRNAs and five circRNAs were selected and the consistent expression trend was found between qRT-PCR detection and RNA-seq. Moreover, the target genes of these differentially expressed ncRNAs (including lncRNAs, circRNAs and miRNAs) were predicted, and significantly enriched in the innate immune response and steroidogenesis pathways. Then, the colocalization and coexpression analyses were conducted to investigate relationships between ncRNAs and mRNAs. The 16 differentially expressed miRNAs targeting 60 differentially expressed mRNAs were identified in granulosa cells at 3 and 6 h post-infection (hpi) and enriched in the MAPK, GnRH, cytokine-cytokine receptor interaction, Toll-like receptor, endocytosis, and oxidative phosphorylation signaling pathways. Additionally, underlying lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA ceRNA networks were then constructed to further understand their interaction during S. Enteritidis infection. Lnc_012227 and novel_circ_0004892 were identified as ceRNAs, which could compete with miR-let-7g-5p and thereby indirectly modulating map3k8 expression to control S. Enteritidis infection. Together, our data thus identified promising candidate ncRNAs responsible for regulating S. Enteritidis infection in the preovulatory follicles of ducks, offering new insights regarding the ovarian transmission of this pathogen.
The long-term effects of the biolicit procedure for original and biosimilar GCSF and EPO products in Hungary
Authors: Hornyák Lajos , Nagy Zsolt , Vathy Fogarassyné Ágnes , Endrei Dóra , Csákvári Tímea , Répásy Balázs , Kresz Sára , Boncz Imre
Published: June 2021

Aim: We aimed to analyse the long-term effects of the biosimilar bids of NEAK regarding GCSF and EPO drugs. Data and Methods: Our analysis is based on the data derived from the nationwide pharmaceutical database of NEAK. The treatment days and reimbursement figures of 12 months periods has been compared, from 01.07.2011-30.06.2014. and 01.07.2017.-30.06.2019. Results: In the 12 months preceding the price competition bid of biosimilar products 13974 patients received G-CSF, 7.49 billion HUF health insurance reimbursement has been paid, 5 years later the turnover of the product increased (314760, 340100 DOT value), whereas the reimbursement decreased (2.03 billion HUF, 1.95 billion HUF respectively). 12 months before the biosimial price competition, 4167 patients were treated with erythropoietin, resulting in 2.33 billion HUF of reimbursements, in the last 2 years the turnover increased (48727, 50813 DOT value respectively) with decreased reimbursement (1.004 billion HUF, 1.002 billion HUF respectively). Conclusions: The long-range analyses price competition bid of biosimilar products revealed that in case of products the health insurance reimbursement decreased despite the elevated turnover in a longer period as well. During the years following the start of the price competition bid the switch from original products to biosimilars could be observed.
Effect of Inorganic Zinc on Selected Immune Parameters in Chicken Blood and Jejunum after A. galli Infection
Authors: Karaffová Viera , Revajová Viera , Dvorožňáková Emília , Grešáková Ľubomíra , Levkut Martin , Ševčíková Zuzana , Herich Róbert , Levkut Mikulas
Published: June 2021

Ascaridiosis in poultry results in a reduction in body weight gain, egg production, as well as microelement levels. Infected poultry have higher demands on feed with the addition of essential elements including zinc. The effects of the infection by Ascaridia galli and the supplementation of inorganic zinc on the immune status of broilers were monitored through evaluation of the relative expression of selected genes (interleukins, IFN-γ, and TNF-α) by real-time PCR, haematology parameters by microscopy, and quantitative changes of lamina propria lymphocytes by flow cytometry in day 7 and day 14 of the study. We observed that the enrichment of the diet with inorganic zinc has a positive effect on the relative percentage of CD4+ lamina propria lymphocytes in the jejunum and on heterophil counts in blood. In addition, it was concluded that inorganic zinc has an anti-inflammatory effect (downregulation of TNF-α and IL-17) and activates IgA-producing cells in the jejunum of chicks infected with A. galli.
Comparative Appraisal of Three Low-Cost GPS Speed Sensors with Different Data Update Frequencies
Authors: Akkamis Mustafa , Keskin Muharrem , Sekerli Yunus Emre
Published: June 2021

Low-cost GPS (Global Positioning System) speed sensors have been available to quantify vehicle speed on different platforms including agricultural tractors in precision agriculture applications such as yield monitoring, variable rate fertilizer and pesticide applications. One of the advances in low-cost GPS receivers is the higher data update frequencies. However, we found no studies on the accuracy of low-cost GPS speed sensors with different update frequencies, especially under variable speed conditions. Thus, this work investigated the effect of the update frequency on the accuracy of low-cost GPS speed sensors under both constant and varying speed conditions. Three GPS speed sensors with update frequencies of 1 Hz, 5 Hz and 7 Hz (GPS1Hz, GPS5Hz and GPS7Hz) were simultaneously tested under the same conditions. A total of 144 tests were conducted on three different days and at three different times of each day with four speed levels and four repetitions. The percent errors were found to be up to 2.3%, 1.8% and 1.4% at constant speeds; up to −47%, −16% and −12% at the increasing speeds and 24%, 6% and 5% at the decreasing speeds, depending on the acceleration and deceleration levels, for GPS1Hz, GPS5Hz and GPS7Hz, respectively. The differences among the error values of the GPS speed sensors were found to be statistically significant (p < 0.05). The GPS speed sensors with higher update frequencies (5 and 7 Hz) provided higher accuracy compared to the one with lower frequency (1 Hz), particularly in the case of higher acceleration conditions. In sum, low-cost GPS speed sensors with higher update frequencies should be used for better accuracy, especially in variable speed conditions.
Agro-Industrial and Urban Compost as an Alternative of Inorganic Fertilizers in Traditional Rainfed Olive Grove under Mediterranean Conditions
Authors: L. de Sosa Laura , Benítez Emilio , Girón Ignacio , Madejón Engracia
Published: June 2021

A three-year field study was conducted to evaluate the impact of two different agro-industrial byproducts on soil properties, provisioning services, olive quality and production in a traditional rainfed olive grove to assess suitable management options for recycling organic wastes and reduce the use of inorganic fertilizers. The organic amendments consisted of compost (AC), made from residues from the olive oil industry (“alperujo”), and biosolid compost (BC), constituted of wastewater sludge and green waste from parks and gardens. The compost addition enhanced carbon storage, available phosphorous and potassium content overtime, whereas no effect was detected on soil hydraulics, yield and olive trees growth, partly due to the high variability encountered among plots. Beneficial effects, especially carbon storage, were more evident during the fourth sampling, where carbon content increased by almost 40% for BC, suggesting that compost effects need to be evaluated in the long term. Strong seasonal changes of most of the physico-chemical parameters were detected, and therefore the effect of the compost could have been partly masked. Establishing a non-climatic variation scenario would be advisable to fully detect compost effects. Our results suggest that different agro-industrial byproducts could be potentially viable and valuable source of fertilization, favoring in this way a circular economy of zero waste.
Monitoring Errors of Semi-Mechanized Coffee Planting by Remotely Piloted Aircraft
Authors: Santana Lucas Santos , Ferraz Gabriel Araújo e Silva , Cunha João Paulo Barreto , Santana Mozarte Santos , Faria Rafael de Oliveira , Marin Diego Bedin , Rossi Giuseppe , Conti Leonardo , Vieri Marco , Sarri Daniele
Published: June 2021

Mechanized operations on terrain slopes can still lead to considerable errors in the alignment and distribution of plants. Knowing slope interference in semi-mechanized planting quality can contribute to precision improvement in decision making, mainly in regions with high slope. This study evaluates the quality of semi-mechanized coffee planting in different land slopes using a remotely piloted aircraft (RPA) and statistical process control (SPC). In a commercial coffee plantation, aerial images were collected by a remotely piloted aircraft (RPA) and subsequently transformed into a digital elevation model (DEM) and a slope map. Slope data were subjected to variance analysis and statistical process control (SPC). Dependent variables analyzed were variations in distance between planting lines and between plants in line. The distribution of plants on all the slopes evaluated was below expected; the most impacted was the slope between 20–25%, implementing 7.8% fewer plants than projected. Inferences about the spacing between plants in the planting row showed that in slopes between 30–40%, the spacing was 0.53 m and between 0 and 15% was 0.55 m. This denotes the compensation of the speed of the operation on different slopes. The spacing between the planting lines had unusual variations on steep slopes. The SCP quality graphics are of lower quality in operations between 30–40%, as they have an average spacing of 3.65 m and discrepant points in the graphics. Spacing variations were observed in all slopes as shown in the SCP charts, and possible causes and implications for future management were discussed, contributing to improvements in the culture installation stage.
Basics for Improved Use of Phages for Therapy
Authors: Serwer Philip , Wright Elena , De La Chapa Jorge , Gonzales Cara
Published: June 2021

Blood-borne therapeutic phages and phage capsids increasingly reach therapeutic targets as they acquire more persistence, i.e., become more resistant to non-targeted removal from blood. Pathogenic bacteria are targets during classical phage therapy. Metastatic tumors are potential future targets, during use of drug delivery vehicles (DDVs) that are phage derived. Phage therapy has, to date, only sometimes been successful. One cause of failure is low phage persistence. A three-step strategy for increasing persistence is to increase (1) the speed of lytic phage isolation, (2) the diversity of phages isolated, and (3) the effectiveness and speed of screening phages for high persistence. The importance of high persistence-screening is illustrated by our finding here of persistence dramatically higher for coliphage T3 than for its relative, coliphage T7, in murine blood. Coliphage T4 is more persistent, long-term than T3. Pseudomonas chlororaphis phage 201phi2-1 has relatively low persistence. These data are obtained with phages co-inoculated and separately assayed. In addition, highly persistent phage T3 undergoes dispersal to several murine organs and displays tumor tropism in epithelial tissue (xenografted human oral squamous cell carcinoma). Dispersal is an asset for phage therapy, but a liability for phage-based DDVs. We propose increased focus on phage persistence—and dispersal—screening.
Evaluation of Benzguinols as Next-Generation Antibiotics for the Treatment of Multidrug-Resistant Bacterial Infections
Authors: Nguyen Hang , Morshed Mahmud , Vuong Daniel , Crombie Andrew , Lacey Ernest , Garg Sanjay , Pi Hongfei , Woolford Lucy , Venter Henrietta , Page Stephen , Piggott Andrew , Trott Darren , Ogunniyi Abiodun
Published: June 2021

Our recent focus on the “lost antibiotic” unguinol and related nidulin-family fungal natural products identified two semisynthetic derivatives, benzguinols A and B, with unexpected in vitro activity against Staphylococcus aureus isolates either susceptible or resistant to methicillin. Here, we show further activity of the benzguinols against methicillin-resistant isolates of the animal pathogen Staphylococcus pseudintermedius, with minimum inhibitory concentration (MIC) ranging 0.5–1 μg/mL. When combined with sub-inhibitory concentrations of colistin, the benzguinols demonstrated synergy against Gram-negative reference strains of Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa (MICs of 1–2 μg/mL in the presence of colistin), whereas the benzguinols alone had no activity. Administration of three intraperitoneal (IP) doses of 20 mg/kg benzguinol A or B to mice did not result in any obvious adverse clinical or pathological evidence of acute toxicity. Importantly, mice that received three 20 mg/kg IP doses of benzguinol A or B at 4 h intervals exhibited significantly reduced bacterial loads and longer survival times than vehicle-only treated mice in a bioluminescent S. aureus murine sepsis challenge model. We conclude that the benzguinols are potential candidates for further development for specific treatment of serious bacterial infections as both stand-alone antibiotics and in combination with existing antibiotic classes.
N-Acetylcysteine (NAC): Impacts on Human Health
Authors: Tenório Micaely Cristina dos Santos , Graciliano Nayara Gomes , Moura Fabiana Andréa , Oliveira Alane Cabral Menezes de , Goulart Marília Oliveira Fonseca
Published: June 2021

N-acetylcysteine (NAC) is a medicine widely used to treat paracetamol overdose and as a mucolytic compound. It has a well-established safety profile, and its toxicity is uncommon and dependent on the route of administration and high dosages. Its remarkable antioxidant and anti-inflammatory capacity is the biochemical basis used to treat several diseases related to oxidative stress and inflammation. The primary role of NAC as an antioxidant stems from its ability to increase the intracellular concentration of glutathione (GSH), which is the most crucial biothiol responsible for cellular redox imbalance. As an anti-inflammatory compound, NAC can reduce levels of tumor necrosis factor-alpha (TNF-α) and interleukins (IL-6 and IL-1β) by suppressing the activity of nuclear factor kappa B (NF-κB). Despite NAC’s relevant therapeutic potential, in several experimental studies, its effectiveness in clinical trials, addressing different pathological conditions, is still limited. Thus, the purpose of this chapter is to provide an overview of the medicinal effects and applications of NAC to human health based on current therapeutic evidence.
Ozone-Based Eye Drops Activity on Ocular Epithelial Cells and Potential Pathogens Infecting the Front of the Eye
Authors: Paduch Roman , Urbanik-Sypniewska Teresa , Kutkowska Jolanta , Chorągiewicz Tomasz , Matysik-Woźniak Anna , Zweifel Sandrine , Czarnek-Chudzik Aleksandra , Załuska Wojciech , Rejdak Robert , Toro Mario Damiano
Published: June 2021

Confirmation of the biological effectiveness of new ophthalmic preparations introduced in the market is an important element in maintaining the safety of using this type of medications. This study aimed to investigate the activity of Ozodrop® on human corneal and conjunctival epithelial cells, as well as its antibacterial and antifungal activity. Cytotoxicity analyses of ocular surface epithelial cells were performed in vitro by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and Neutral Red uptake assays. The level of nitric oxide released by the cells was assessed by the Griess method. The reduction of the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical by the tested formulation was analyzed. Microbiological tests were also performed. It was found that the Ozodrop® preparation exhibited biological activity, but was less active than the reference antibiotics and the anti-yeast agent. The cytotoxic activity of the Ozodrop® formulation was dependent on the time of cell exposure to it. No toxic effect was observed in the short-term, for up to 3 h. It appeared after 24 h of exposure of the cells to the preparation. The drops showed antioxidant activity in the specified concentration range. They also stimulated the release of nitric oxide, mainly by corneal epithelial cells. The Ozodrop® formulation exhibits biological activity that can be considered useful in the treatment of infections in the front part of the eye.
A Novel Strategy to Produce a Soluble and Bioactive Wheat Bran Ingredient Rich in Ferulic Acid
Authors: Martín-Diana Ana Belén , Tomé-Sánchez Irene , García-Casas María Jesús , Martínez-Villaluenga Cristina , Frías Juana , Rico Daniel
Published: June 2021

Wheat bran (WB) is a byproduct from the milling industry that contains bioactive compounds beneficial to human health. The aim of this work was on the one hand, increasing extractability of antioxidant and anti-inflammatory compounds (specifically ferulic acid, FA), through enzymatic hydrolysis combined with hydrothermal treatment (HT) and high hydrostatic pressure (HHP). On the other hand, enhancing the stability of final ingredient applying spray-drying (SPD) and microencapsulation (MEC). The use of HT increased FA, total phenolics (TP), and antioxidant capacity (AC) in WB hydrolysates, regardless the HT duration. However, the HT tested (30 min, HT30) produced a loss in anti-inflammatory activity (AIA). The combination of HT (15 min, HT15) with HHP increased AIA of the WB. SPD enhanced the TP yield in WB with no significant effect of inlet temperature (up to 140 °C) on phenolic profile mainly composed of trans-FA and smaller amounts of cis-FA and apigenin diglucosides. SPD caused a temperature-dependent increase in AC (160 °C > 140 °C > 130 °C). SPD inlet temperatures affected total solids yield (from 22 to 36%), with the highest values at 140 °C. The use of HHP in combination with HT resulted in >2-fold increase in total solids yield.
Integrating Convolutional Neural Network and Multiresolution Segmentation for Land Cover and Land Use Mapping Using Satellite Imagery
Authors: Atik Saziye Ozge , Ipbuker Cengizhan
Published: June 2021

Depletion of natural resources, population growth, urban migration, and expanding drought conditions are some of the reasons why environmental monitoring programs are required and regularly produced and updated. Additionally, the usage of artificial intelligence in the geospatial field of Earth observation (EO) and regional land monitoring missions is a challenging issue. In this study, land cover and land use mapping was performed using the proposed CNN–MRS model. The CNN–MRS model consisted of two main steps: CNN-based land cover classification and enhancing the classification with spatial filter and multiresolution segmentation (MRS). Different band numbers of Sentinel-2A imagery and multiple patch sizes (32 × 32, 64 × 64, and 128 × 128 pixels) were used in the first experiment. The algorithms were evaluated in terms of overall accuracy, precision, recall, F1-score, and kappa coefficient. The highest overall accuracy was obtained with the proposed approach as 97.31% in Istanbul test site area and 98.44% in Kocaeli test site area. The accuracies revealed the efficiency of the CNN–MRS model for land cover map production in large areas. The McNemar test measured the significance of the models used. In the second experiment, with the Zurich Summer dataset, the overall accuracy of the proposed approach was obtained as 92.03%. The results are compared quantitatively with state-of-the-art CNN model results and related works.