bluuebunny/crossref_metadata_2025 huggingface dataset:
Public and Private Standards in Crop Production: Their Role in Ensuring Safety and Sustainability
Authors: Squatrito Salvatore , Arena Elena , Palmeri Rosa , Fallico Biagio
Published: January 2020

From the comparison of regulations and/or standards for the organic, conventional and/or integrated citrus production method and a voluntary certification, it emerges that farms certified with voluntary non-regulated certification systems, such as the IFA FV GLOBALG.A.P, are obliged to take into account the highest number of aspects, reported in a more complete register, than the organic ones. Moreover, this is also supported by a continuous-time planned process of revision and updating of the applicable versions of the standard. The environmental impact of the food production, the safety aspects of food products, as well as the health, ethics, and safety aspects of workers, are largely considered and inspected in the GLOBALG.A.P., while the organic system, despite the IFOAM suggestions and indications, is only considered partially. This means that, from a practical point of view, the organic product can be considered “clean and safe”, but not more environmentally friendly than the GLOBALG.A.P. products.
Environmental Impacts on the Performance of Solar Photovoltaic Systems
Authors: Mustafa Ramadan J. , Gomaa Mohamed R. , Al-Dhaifallah Mujahed , Rezk Hegazy
Published: January 2020

This study scrutinizes the reliability and validity of existing analyses that focus on the impact of various environmental factors on a photovoltaic (PV) system’s performance. For the first time, four environmental factors (the accumulation of dust, water droplets, birds’ droppings, and partial shading conditions) affecting system performance are investigated, simultaneously, in one study. The results obtained from this investigation demonstrate that the accumulation of dust, shading, and bird fouling has a significant effect on PV current and voltage, and consequently, the harvested PV energy. ‘Shading’ had the strongest influence on the efficiency of the PV modules. It was found that increasing the area of shading on a PV module surface by a quarter, half, and three quarters resulted in a power reduction of 33.7%, 45.1%, and 92.6%, respectively. However, results pertaining to the impact of water droplets on the PV panel had an inverse effect, decreasing the temperature of the PV panel, which led to an increase in the potential difference and improved the power output by at least 5.6%. Moreover, dust accumulation reduced the power output by 8.80% and the efficiency by 11.86%, while birds fouling the PV module surface was found to reduce the PV system performance by about 7.4%.
Social Housing: An Appraisal Model of the Economic Benefits in Urban Regeneration Programs
Authors: Della Spina Lucia , Calabrò Francesco , Rugolo Alessandro
Published: January 2020

The decision-making process relating to Social Housing (SH) policies and projects involves social and urban transformations and is consequently linked to urban planning, urban regeneration projects, the dynamics of the real estate market and cooperation between public and private developers. Furthermore, this decision-making process must be supported by assessments relating to economic feasibility and assessments on social and environmental sustainability. The paper illustrates a decision support evaluation model for the implementation of integrated urban redevelopment programs related to Social Housing interventions to be implemented in PPP. The model is based on the search for an economic balance between the interests of the parties involved, with the aim of maximizing the share of housing in SH by minimizing the public contribution quota. The model was developed on a degraded settlement of Public Residential Construction, the subject of a wider urban regeneration program in the Municipality of Reggio Calabria (Italy). Considering the financial feasibility constraint for the developer and the conditions of the local real estate market, with the risk of the investment attached to it, the model makes it possible to verify the economic sustainability and the financial feasibility of the interventions in SH through the estimation of: (i) The profit of the developer/investor; (ii) the trade-in value to be paid to the developer against the investment; (iii) the maximum share of SH to be carried out in development. The research and the results obtained highlight the utility of the model and the ease of use in the programming phase, in relation to urban regeneration programs that involve interventions in SH.
Trends and Characteristics of Inter-Provincial Migrants in Mainland China and Its Relation with Economic Factors: A Panel Data Analysis from 2011 to 2016
Authors: Shi Lishuo , Chen Wen , Xu Jiaqi , Ling Li
Published: January 2020

For areas facing challenges associated with migration, information about the number of migrants and their demographic characteristics is needed to formulate regional development planning. This study analyzed the trends and characteristics of inter-provincial migrants in provinces in mainland China and related economic factors using panel regression models. The results showed that the number of inter-provincial migrants had increased in provincial municipalities, as had the proportions of female and elderly migrants. A higher annual net migration rate was associated with slower growth rate of real gross domestic product (RGDP) per capita and faster growth rates of the tertiary and secondary industry GDPs. The higher proportion of female migrants was related to the faster growth rate of the tertiary industry GDP and the lower proportion of the secondary industry in GDP. The proportion of youth migrants was positively related to educational investment, while the proportion of elderly migrants was positively related to financial expenditure per capita on culture and recreation. These empirical results were robust across different estimation methods, except the result about the proportion of elderly migrants. These findings further reveal the association between inter-provincial migration and economy and provide policy reference for the management of migrants.
Sustainable Cruise Tourism in Marine World Heritage Sites
Authors: Cerveny Lee K. , Miller Anna , Gende Scott
Published: January 2020

Cruise-ship tourism is one of the fastest growing industry sectors, with itineraries that regularly visit marine parks and protected areas. UNESCO Marine World Heritage (MWH) Sites feature some of the world’s most exceptional ecosystems, resulting in some cruise lines targeting these sites. To understand the extent of cruise ship visitation and determine perceptions of cruise ship sustainability within and across environmental, economic, and sociocultural dimensions, we conducted an online survey of 45 (out of 50) sites. The survey included responses about the characteristics of cruise ship visitation, strategies for sustainably managing ships, and ideas for encouraging sustainable practices. Among the 45 respondents, 30 (67%) indicated that their MWH site hosts cruise ships or cruise ship passengers, and 25 sites have cruise ships that enter the protected area marine waters. Most sites (62%) indicated an increase in cruise visitation over the last three years. While most sites regulate ballast water (73%) and wastewater (73%) discharge, common concerns focused on ship air emissions and wildlife interactions. Lack of funds generated by cruise ships toward community infrastructure was noted. MWH site managers expressed interest in developing site networks to facilitate sharing of ideas as a first step for increasing sustainability across all sites.
Framing Descriptive Norms as Self-Benefit Versus Environmental Benefit: Self-Construal’s Moderating Impact in Promoting Smart Energy Devices
Authors: Mingolla Carla , Hudders Liselot , Cauberghe Veroline
Published: January 2020

Recently, awareness has been raised concerning the importance of sustainable energy use. Nevertheless, many obstacles must be overcome to change individuals’ energy consumption habits. This study examines how a message should be framed to convince individuals to purchase a smart energy device that provides feedback on household energy use. As such, this device can assist households in adjusting their energy-wasting habits. Through two experimental studies, this paper examines how a descriptive normative message, indicating that the majority of US households have already purchased a smart energy device, can increase individuals’ intention to purchase the device. Both studies consider the moderating influence of the self-construal, which refers to individuals’ consideration of themselves as either part of a group (interdependent self-construal) or independent from others (independent self-construal). The first study (n = 231) reveals that a descriptive norm (versus no norm) leads to a higher purchase intention through an enhanced normative influence regardless of participants’ self-construal. The second study (n = 128) adds to the finding that combining a descriptive norm with a self-benefit (versus environmental) frame more strongly impacts the purchase intent of individuals with a dominant independence. No significant differences are identified between the two benefit frames’ effectiveness among individuals with a dominant interdependence.
A Process Oriented MCDM Approach to Construct a Circular Economy Composite Index
Authors: Garcia-Bernabeu Ana , Hilario-Caballero Adolfo , Pla-Santamaria David , Salas-Molina Francisco
Published: January 2020

The purpose of this contribution is to develop a Circular Economy Composite indicator to benchmark EU countries performance. Europe is at the forefront of the global transition towards a sustainable and circular economy. To this end, the European Commission has launched in 2015 a Circular Economy Action Plan including a monitoring framework to measure progress and to assess the effectiveness of initiatives towards the circular economy in the European Union (EU) and Member States. Still, this monitoring framework lacks a composite indicator at the national level to aggregate the circular economy dimensions into a single summary indicator. Although there is a wide range of sustainability composite indicators, no aggregate circular economy index exits to this date. We use a multi-criteria approach to construct a circular economy composite index based on TOPSIS (Technique for Order Preferences by Similarity to Ideal Solutions) methodology. In addition, we introduce a novel aggregation methodology for building a composite indicator where different levels of compensability for the distances to the ideal and anti-ideal (or negative-ideal) values of each indicator are considered. In order to illustrate the advantages of this proposal, we have applied it to evaluate the Circular Economy performance of EU Member States for the year 2016. This proposal can be a valuable tool for identifying areas in which the countries need to concentrate their efforts to boost their circular economy performance.
An Empirical Study on Distance Education and Job Match
Authors: Li Fengliang , Wang Liang
Published: January 2020

Job match has always been the focus of educational research. However, current empirical studies are limited to the analysis of face-to-face education, and there’s no empirical study focusing on the job match of distance education. To fill the gap in this research field, this study analyzes the distance learners in China to demonstrate the relationship between distance education and job match by using the data from a nationwide household survey. The empirical results involve two significant findings. Firstly, distance learners and face-to-face learners have no significant difference in job match. This study attempts to explain this with the human capital theory, that is, distance learners and face-to-face learners have no difference in obtaining their specific human capital, so they both prefer to work on a position characterized by job match. Secondly, job mismatch has no significant negative effect on the income of distance learners. This study attempts to explain this with the screening theory, that is, though distance education would improve the learners’ specific human capital, it still acts as a diploma signal, to some extent, in China, thus making it impossible for the specific human capital obtained by distance learners to transform into a superiority in income.
Digitally Printed AgNPs Doped TiO2 on Commercial Porcelain-Grès Tiles: Synergistic Effects and Continuous Photocatalytic Antibacterial Activity
Authors: Bianchi Claudia Letizia , Cerrato Giuseppina , Bresolin Bianca Maria , Djellabi Ridha , Rtimi Sami
Published: January 2020

In the present study, we use commercial digitally printed ceramic tiles, functionnalized by AgNPs doped micro–TiO2, to investigate the mechanism of Ag in the continouos photocatalytic antibacterial activity. The novelty of the research lies in the attempt to understand the mechanism of Ag, supported on TiO2, able to exhibit the same antibacterial activity of a standard system containing Ag species, but here, totally embedded on the tile surface, and thus not free to move and damage the bacteria cell. UV/vis diffuse reflectance spectroscopy (DRS) of AgNPs–TiO2 tiles indicated an enhanced visible light response, wherein a new absorption band was produced around 18,000–20,000 cm−1 (i.e., in the 400–600 nm range) owing to the surface plasmon resonance (SPR) of AgNPs. The antibacterial photocatalytic experiments were conducted towards the inactivation of E. coli under solar light and indoor light. It was found that the degradation speed of E. coli in the presence of AgNPs–TiO2 tiles is solar light-intensity depending. This justifies the semiconductor behavior of the material. Furthermore, the AgNPs–TiO2 tiles exhibit a high ability for the inactivation of E. coli at a high load (104–107 colony-forming unit (CFU)/mL). Additionally, AgNPs–TiO2 tiles showed a remarkable antibacterial activity under indoor light, which confirms the good photocatalytic ability of such tiles. On the basis of the reactive oxygen species (ROS) quenching experiments, O2•− species and h+ were more reactive for the inactivation of E. coli rather than •OH species. This is because of the different lifetime (bacteria are more likely oxidized by ROS with longer lifetime); in fact, O2•− and h+ exhibit a longer lifetime compared with •OH species. The generation of H2O2 as the most stable ROS molecule was also suggested.
Constitutive Model of Stress-Dependent Seepage in Columnar Jointed Rock Mass
Authors: Niu Zihao , Zhu Zhende , Que Xiangcheng
Published: January 2020

Columnar jointed rock mass (CJRM) is a highly symmetrical natural fractured structure. As the rock mass of the dam foundation of the Baihetan Hydropower Station, the study of its permeability anisotropy is of great significance to engineering safety. Based on the theory of composite mechanics and Goodman’s joint superposition principle, the constitutive model of joints of CJRM is derived according to the Quadrangular prism, the Pentagonal prism and the Hexagonal prism model; combined with Singh’s research results on intermittent joint stress concentration, considering column deflection angles, the joint constitutive model of CJRM in three-dimensional space is established. For the CJRM in the Baihetan dam site area, the Quadrangular prism, the Pentagonal prism and the Hexagonal prism constitutive models were used to calculate the permeability coefficients of CJRM under different deflection angles. The permeability anisotropy characteristics of the three models were compared and verified by numerical simulation results. The results show that the calculation results of the Pentagonal prism model are in good agreement with the numerical simulation results. The variation of permeability coefficient under different confining pressures is compared, and the relationship between permeability coefficient and confining pressure is obtained, which accords with the negative exponential function and conforms to the general rule of joint seepage.
Modelling of Autonomous Search and Rescue Missions by Interval-Valued Neutrosophic WASPAS Framework
Authors: Semenas Rokas , Bausys Romualdas
Published: January 2020

The application of autonomous robots in search and rescue missions represents a complex task which requires a robot to make robust decisions in unknown and dangerous environments. However, imprecise robot movements and small measurement errors obtained by robot sensors can have an impact on the autonomous environment exploration quality, and therefore, should be addressed while designing search and rescue (SAR) robots. In this paper, a novel frontier evaluation strategy is proposed, that address technical, economic, social, and environmental factors of the sustainable environment exploration process, and a new extension of the weighted aggregated sum product assessment (WASPAS) method, modelled under interval-valued neutrosophic sets (IVNS), is introduced for autonomous mobile robots. The general-purpose Pioneer 3-AT robot platform is applied in simulated search and rescue missions, and the conducted experimental assessment shows the proposed method efficiency in commercial and public-type building exploration. By addressing the estimated measurement errors in the initial data obtained by the robot sensors, the proposed decision-making framework provides additional reliability for comparing and ranking candidate frontiers. The interval-valued multi-criteria decision-making method combined with the proposed frontier evaluation strategy enables the robot to exhaustively explore and map smaller SAR mission environments as well as ensure robot safety and efficient energy consumption in relatively larger public-type building environments.
On Neutrosophic Extended Triplet LA-hypergroups and Strong Pure LA-semihypergroups
Authors: Hu Minghao , Smarandache Florentin , Zhang Xiaohong
Published: January 2020

We introduce the notions of neutrosophic extended triplet LA-semihypergroup, neutrosophic extended triplet LA-hypergroup, which can reflect some symmetry of hyperoperation and discuss the relationships among them and regular LA-semihypergroups, LA-hypergroups, regular LA-hypergroups. In particular, we introduce the notion of strong pure neutrosophic extended triplet LA-semihypergroup, get some special properties of it and prove the construction theorem about it under the condition of asymmetry. The examples in this paper are all from Python programs.
Efficient Lattice CP-ABE AC Scheme Supporting Reduced-OBDD Structure for CCN/NDN
Authors: Affum Eric , Zhang Xiasong , Wang Xiaofen , Ansuura John Bosco
Published: January 2020

In line with the proposed 5th Generation network, content centric network/named data networking (CCN/NDN) has been offered as one of the promising paradigms to cope with the communication needs of future realistic network communications. CCN/NDN allows network communication based on content names and also allows users to obtain information from any of the nearest intermediary caches on the network. Due to that, the ability of cached content to protect itself is essential since contents can be cached on any node everywhere, and publishers may not have total control over their own published data. The attribute based encryption (ABE) scheme is a preferable approach, identified to enable cached contents to be self-secured since it has a special property of encryption with policies. However, most of the proposed ABE schemes for CCN/NDN suffer from some loopholes. They are not flexible in the expression of access policy, they are inefficient, they are based on bilinear maps with pairings, and they are vulnerable to quantum cryptography algorithms. Hence, we propose the ciphertext policy attribute based encryption access control (CP-ABE AC) scheme from a lightweight ideal lattice based on ring learning with error (R-LWE) problem, and demonstrated its use in practical applications. The proposed scheme is proved to be secure and efficient under the decision ring LWE problem in the selective set model. To achieve an efficient scheme, we used an efficient trapdoor technique and the access tree representation of access structure describing the access policies was modified into a new structure, based on a reduced ordered binary decision diagram (reduce-OBDD). This access structure can support Boolean operations such as AND, NOT, OR, and threshold gates. The final result showed that the proposed scheme was secure and efficient for applications, thereby supporting CCN/NDN as a promising paradigm.
Role of Shiga Toxins in Cytotoxicity and Immunomodulatory Effects of Escherichia coli O157:H7 during Host-Bacterial Interactions in vitro
Authors: Bruballa Andrea Cecilia , Shiromizu Carolina Maiumi , Bernal Alan Mauro , Pineda Gonzalo Ezequiel , Sabbione Florencia , Trevani Analia Silvina , Bentancor Leticia Verónica , Ramos María Victoria , Fernández-Brando Romina Jimena , Muñoz Manuel Javier , Palermo Marina Sandra
Published: January 2020

Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this aim, macrophage-differentiated THP-1 cells and the intestinal epithelial cell line HCT-8 were incubated with E. coli O157:H7. A time course analysis of cellular and bacterial survival, Stx2 production, stx2 transcription, and cytokine secretion were analyzed in both human cell lines. We demonstrated that macrophages are able to internalize and kill EHEC. Simultaneously, Stx2 produced by internalized bacteria played a major role in macrophage death. In contrast, HCT-8 cells were completely resistant to EHEC infection. Besides, macrophages and HCT-8 infected cells produce IL-1β and IL-8 inflammatory cytokines, respectively. At the same time, bacterial stx2-specific transcripts were detected only in macrophages after EHEC infection. The interplay between bacteria and host cells led to Stx production, triggering of inflammatory response and cell damage, all of which could contribute to a severe outcome after EHEC infections.
Product Development Partnerships: Delivering Innovation for the Elimination of African Trypanosomiasis?
Authors: Taylor Emma Michelle , Smith James
Published: January 2020

African trypanosomiasis has been labelled as a ‘tool-deficient’ disease. This article reflects on the role that Product Development Partnerships (PDPs) have played in delivering new tools and innovations for the control and elimination of the African trypanosomiases. We analysed three product development partnerships—DNDi, FIND and GALVmed—that focus on delivering new drugs, diagnostic tests, and animal health innovations, respectively. We interviewed key informants within each of the organisations to understand how they delivered new innovations. While it is too early (and beyond the scope of this article) to assess the role of these three organisations in accelerating the elimination of the African trypanosomiases, all three organisations have been responsible for delivering new innovations for diagnosis and treatment through brokering and incentivising innovation and private sector involvement. It is doubtful that these innovations would have been delivered without them. To varying degrees, all three organisations are evolving towards a greater brokering role, away from only product development, prompted by donors. On balance, PDPs have an important role to play in delivering health innovations, and donors need to reflect on how best to incentivise them to focus and continue to deliver new products.
Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage
Authors: Yang Denghui , Wang Zhaofei , Ma Jingjiao , Fu Qiang , Wu Lifei , Wang Hengan , Wang Shaohui , Yan Yaxian , Sun Jianhe
Published: January 2020

The CRISPR/Cas system protects bacteria against bacteriophage and plasmids through a sophisticated mechanism where cas operon plays a crucial role consisting of cse1 and cas3. However, comprehensive studies on the regulation of cas3 operon of the Type I-E CRISPR/Cas system are scarce. Herein, we investigated the regulation of cas3 in Escherichia coli. The mutation in gcvP or crp reduced the CRISPR/Cas system interference ability and increased bacterial susceptibility to phage, when the casA operon of the CRISPR/Cas system was activated. The silence of the glycine cleavage system (GCS) encoded by gcvTHP operon reduced cas3 expression. Adding N5, N10-methylene tetrahydrofolate (N5, N10-mTHF), which is the product of GCS-catalyzed glycine, was able to activate cas3 expression. In addition, a cAMP receptor protein (CRP) encoded by crp activated cas3 expression via binding to the cas3 promoter in response to cAMP concentration. Since N5, N10-mTHF provides one-carbon unit for purine, we assumed GCS regulates cas3 through associating with CRP. It was evident that the mutation of gcvP failed to further reduce the cas3 expression with the crp deletion. These results illustrated a novel regulatory pathway which GCS and CRP co-regulate cas3 of the CRISPR/Cas system and contribute to the defence against invasive genetic elements, where CRP is indispensable for GCS regulation of cas3 expression.
Single Amino Acid Substitutions in the Cucumber Mosaic Virus 1a Protein Induce Necrotic Cell Death in Virus-Inoculated Leaves without Affecting Virus Multiplication
Authors: Tian Ainan , Miyashita Shuhei , Ando Sugihiro , Takahashi Hideki
Published: January 2020

When Arabidopsis thaliana ecotype Col-0 was inoculated with a series of reassortant viruses created by exchanging viral genomic RNAs between two strains of cucumber mosaic virus (CMV), CMV(Y), and CMV(H), cell death developed in the leaves inoculated with reassortant CMV carrying CMV(H) RNA1 encoding 1a protein, but not in noninoculated upper leaves. In general, cell death in virus-infected plants is a critical event for virus survival because virus multiplication is completely dependent on host cell metabolism. However, interestingly, this observed cell death did not affect either virus multiplication in the inoculated leaves or systemic spread to noninoculated upper leaves. Furthermore, the global gene expression pattern of the reassortant CMV-inoculated leaves undergoing cell death was clearly different from that in hypersensitive response (HR) cell death, which is coupled with resistance to CMV. These results indicated that the observed cell death does not appear to be HR cell death but rather necrotic cell death unrelated to CMV resistance. Interestingly, induction of this necrotic cell death depended on single amino acid substitutions in the N-terminal region surrounding the methyltransferase domain of the 1a protein. Thus, development of necrotic cell death might not be induced by non-specific damage as a result of virus multiplication, but by a virus protein-associated mechanism. The finding of CMV 1a protein-mediated induction of necrotic cell death in A. thaliana, which is not associated with virus resistance and HR cell death, has the potential to provide a new pathosystem to study the role of cell death in virus–host plant interactions.
Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins
Authors: Chang Hao , Siarot Lowela , Matsuura Ryosuke , Lo Chieh-Wen , Sato Hirotaka , Otsuki Hiroyuki , Aida Yoko
Published: January 2020

Viral protein R (Vpr) is an accessory protein found in various primate lentiviruses, including human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2) as well as simian immunodeficiency viruses (SIVs). Vpr modulates many processes during viral lifecycle via interaction with several of cellular targets. Previous studies showed that HIV-1 Vpr strengthened degradation of Mini-chromosome Maintenance Protein10 (MCM10) by manipulating DCAF1-Cul4-E3 ligase in proteasome-dependent pathway. However, whether Vpr from other primate lentiviruses are also associated with MCM10 degradation and the ensuing impact remain unknown. Based on phylogenetic analyses, a panel of primate lentiviruses Vpr/x covering main virus lineages was prepared. Distinct MCM10 degradation profiles were mapped and HIV-1, SIVmus and SIVrcm Vprs induced MCM10 degradation in proteasome-dependent pathway. Colocalization and interaction between MCM10 with these Vprs were also observed. Moreover, MCM10 2-7 interaction region was identified as a determinant region susceptible to degradation. However, MCM10 degradation did not alleviate DNA damage response induced by these Vpr proteins. MCM10 degradation by HIV-1 Vpr proteins was correlated with G2/M arrest, while induction of apoptosis and oligomerization formation of Vpr failed to alter MCM10 proteolysis. The current study demonstrated a distinct interplay pattern between primate lentiviruses Vpr proteins and MCM10.
Cross-Protection Induced by a A/MAY/97 Emergency Vaccine Against Intra-Serotype Heterologous Challenge with a Foot-and-Mouth Disease Virus from the A/ASIA/G-VII Lineage
Authors: Dekker Aldo , Sanz-Bernardo Beatriz , Singanallur Nagendrakumar Balasubramanian , Ludi Anna B. , Horsington Jacquelyn , Eblé Phaedra L. , King Donald P. , Vosloo Wilna
Published: January 2020

Since 2015, outbreaks of foot-and-mouth disease (FMD) in the Middle East have been caused by a new emerging viral lineage, A/ASIA/G-VII. Invitro vaccine matching data indicated that this virus poorly matched (low r1-value) with vaccines that were being used in the region as well as most other commercially available vaccines. The aim of this study was to assess the performance of two candidate vaccines against challenge with a representative field virus from the A/ASIA/G-VII lineage. The results from an initial full dose protection study provided encouraging data for the A/MAY/97 vaccine, while the A22/IRQ/64 vaccine only protected 2/7 vaccinated animals. In view of these promising results, this vaccine was tested in a potency test (PD50) experiment in which 5 cattle were vaccinated with a full dose, 5 cattle with a 1/3 dose and 5 cattle with a 1/9 dose of vaccine. At 21 days post vaccination these vaccinated cattle and 3 control cattle were challenged intradermolingually with a field isolate from the A/ASIA/G-VII lineage. The intra-serotype heterologous potency test resulted in an intra-serotype heterologous potency of 6.5 PD50/dose. These data support previous studies showing that a high potency emergency vaccine can protect against clinical disease when challenged with a heterologous strain of the same serotype, indicating that not only the r1-value of the vaccine, but also the homologous potency of a vaccine should be taken into account when advising vaccines to control an outbreak.
HPV-Specific Systemic Antibody Responses and Memory B Cells are Independently Maintained up to 6 Years and in a Vaccine-Specific Manner Following Immunization with Cervarix and Gardasil in Adolescent and Young Adult Women in Vaccination Programs in Italy
Authors: Nicoli Francesco , Mantelli Barbara , Gallerani Eleonora , Telatin Valentina , Bonazzi Irene , Marconi Peggy , Gavioli Riccardo , Gabrielli Liliana , Lazzarotto Tiziana , Barzon Luisa , Palù Giorgio , Caputo Antonella
Published: January 2020

Human papillomavirus (HPV) persistent infections are associated with cervical cancer and other HPV-related diseases and tumors. Thus, the characterization of long lasting immunity to currently available HPV vaccines is important. A total of 149 female subjects vaccinated with Cervarix or Gardasil participated to the study and they were stratified according to age (10–12-year-old and 16–20-year-old). Humoral immune responses (IgG and neutralizing antibody titers, antibody avidity) and circulating memory B cells were analyzed after an average of 4–6 years from the third immunization. The humoral responses against HPV-16 and HPV-18 (and HPV-6 and HPV-11 for Gardasil) were high in both age groups and vaccines up to six years from the third dose. However, Cervarix induced significantly higher and more persistent antibody responses, while the two vaccines were rather equivalent in inducing memory B cells against HPV-16 and HPV-18. Moreover, the percentage of subjects with vaccine-specific memory B cells was even superior among Gardasil vaccinees and, conversely, Cervarix vaccinated individuals with circulating antibodies, but undetectable memory B cells were found. Finally, a higher proportion of Cervarix-vaccinated subjects displayed cross-neutralizing responses against non-vaccine types HPV-31 and HPV-45. Gardasil and Cervarix may, thus, differently affect long-lasting humoral immunity from both the quantitative and qualitative point of view.
Investigation of Melioidosis Outbreak in Pig Farms in Southern Thailand
Authors: Kwanhian Wiyada , Jiranantasak Treenate , Kessler Aleeza T. , Tolchinsky Bryn E. , Parker Sarah , Songsri Jirarat , Wisessombat Suebtrakool , Pukanha Kawinsaya , Testamenti Vincentius A. , Khrongsee Pacharapong , Sretrirutchai Somporn , Kaewrakmuk Jedsada , Tangpong Jitbanjong , Tuanyok Apichai
Published: January 2020

Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a potentially life-threatening infection that can affect humans and a wide variety of animals in the tropics. In December 2017, a swine melioidosis case was discovered during a meat inspection at a privately-owned slaughterhouse in Nakhon Si Thammarat Province in southern Thailand. The infection, which continued for several months, caused a dispute about where the disease began. An environmental investigation into two farms—both involved in raising the first infected pig—ensued. Through genetic analysis, the investigation revealed that a contaminated water supply at one farm was the probable source of infection. The three local sequence types identified in the investigation were types 51, 298 and 392.
Formulation and Characterization of a Heterotrophic Nitrification-Aerobic Denitrification Synthetic Microbial Community and its Application to Livestock Wastewater Treatment
Authors: Zhang Qi-yu , Yang Ping , Liu Lai-sheng , Liu Zeng-jin
Published: January 2020

There have been many studies on single strains in wastewater treatment and a new synthetic microbial community was prepared in this study, which provides a reference for the application of heterotrophic nitrification-aerobic denitrification in actual wastewater treatment. The growth period distribution of the composite bacteria was determined by plotting growth curves with different sole nitrogen sources, and the influence of the carbon source, carbon to nitrogen ratio (C/N) ratio, pH, and temperature on ammonia removal by the composite heterotrophic nitrifying-aerobic denitrifying strain was investigated. The optimal conditions for the heterotrophic nitrification process were sodium citrate as the carbon source, a C/N ratio of 10, a pH of 7, and a temperature of 30 °C, and only trace amounts of nitrate and nitrite were observed during the process. When the sequencing batch reactor (SBR) of a pig farm wastewater treatment plant was inoculated with the synthetic microbial community, the average removals of the chemical oxygen demand (COD) and ammonia nitrogen in the effluent were 92.61% and 20.56%, respectively. From the results, the synthetic microbial community was able to simultaneously perform heterotrophic nitrification-aerobic denitrification indicating great potential for full-scale applications.
Modeling River Ice Breakup Dates by k-Nearest Neighbor Ensemble
Authors: Sun Wei , Lv Ying , Li Gongchen , Chen Yumin
Published: January 2020

Forecasting of river ice breakup timing is directly related to the local ice-caused flooding management. However, river ice forecasting using k-nearest neighbor (kNN) algorithms is limited. Thus, a kNN stacking ensemble learning (KSEL) method was developed and applied to forecasting breakup dates (BDs) for the Athabasca River at Fort McMurray in Canada. The kNN base models with diverse inputs and distance functions were developed and their outputs were further combined. The performance of these models was examined using the leave-one-out cross validation method based on the historical BDs and corresponding climate and river conditions in 1980–2015. The results indicated that the kNN with the Chebychev distance functions generally outperformed other kNN base models. Through the simple average methods, the ensemble kNN models using multiple-type (Mahalanobis and Chebychev) distance functions had the overall optimal performance among all models. The improved performance indicates that the kNN ensemble is a promising tool for river ice forecasting. The structure of optimal models also implies that the breakup timing is mainly linked with temperature and water flow conditions before breakup as well as during and just after freeze up.
Study on Runoff and Infiltration for Expansive Soil Slopes in Simulated Rainfall
Authors: Lei Wenkai , Dong Hongyuan , Chen Pan , Lv Haibo , Fan Liyun , Mei Guoxiong
Published: January 2020

In order to understand the hydrological process of expansive soil slopes, simulated rainfall experiments were conducted to study the effects of slope gradient and initial soil moisture content on runoff and infiltration for expansive soil slopes located in south China. The field program consisted of four neighboring slopes (70%, 47%, 32%, and 21%) instrumented by a runoff collection system and moisture content sensors (EC-5). Results from the monitored tests indicate that there was delay in the response of surface runoff. The runoff initiation time decreased with initial soil water content and increasing slope gradient. After the generation of runoff, the cumulative runoff per unit area and the runoff rate increased linearly and logarithmically with time, respectively. The greater the initial soil moisture content was, the smaller the influence of slope gradient on runoff. A rainfall may contribute from 39% to about 100% of its total rainfall as infiltration, indicating that infiltration remained an important component of the rainwater falling on the slope, despite the high initial soil water content. The larger the initial sealing degree of slope surface was the smaller the cumulative infiltration per unit area of the slope. However, the soil moisture reaction was more obvious. The influence of inclination is no longer discernible at high initial moisture levels. The greater the initial soil moisture content and the smaller the slope gradient, the weaker was the change of soil water content caused by simulated rainfall. The influence of initial soil moisture content and slope gradient on the processes of flow and changes of soil water content identified in this study may be helpful in the surface water control for expansive soil slopes.
Experimental Investigation of Substrate Shock and Environmental Ammonium Concentration on the Stability of Ammonia-Oxidizing Bacteria (AOB)
Authors: Keerio Hareef Ahmed , Bae Wookeun
Published: January 2020

A wastewater treatment plant (WWTP) frequently encounters fluctuation in ammonium concentration or flow rate (Q), which may affect the stability of ammonium oxidizing bacteria (AOB). In this study, two continuous stirred tank reactors (CSTRs) were operated for 588 days and ammonium concentration was varied at various steady-state conditions. There was no inhibition observed in CSTR operation and AOB acclimated once at a certain ammonium concentration. Cells at an acclimated steady-state concentration (200 mgTAN/L from R(A) and 1000 mgTAN/L from R(B)) were extracted to perform a batch test at operating conditions, and self-inhibition behavior was observed in the batch reaction. In CSTR operation, the environmental ammonium concentration was varied and the specific oxygen uptake rate (SOUR) value was estimated from daily profile data and compared with batch reaction. In the CSTR operation as a substitute for self-inhibition, the SOUR was shifted towards the maximum specific oxygen uptake rate (SOURmax) and no self-inhibition was observed. For further justification of the CSTR’s stability, several total ammonium nitrogen (TAN) concentrations (range from ~−106 to ~+2550 mgTAN/L) were directly added to interrupt the stability of the process. As a substitute for any effect on the SOUR, the CSTRs were recovered back to the original stable steady-state conditions without varying the operational conditions.
Assessing the Impact of Climate Change on Groundwater Quality of the Shallow Coastal Aquifer of Eastern Dahomey Basin, Southwestern Nigeria
Authors: Aladejana Jamiu A. , Kalin Robert M. , Sentenac Philippe , Hassan Ibrahim
Published: January 2020

Despite the increasing interest in climate change and water security, research linking climate change and groundwater quality is still at an early stage. This study explores the seasonal effect of the change in biogeochemical process for the redox-sensitive ions and metals Fe2+, Mn2+, SO42−, and NO3− to assess the groundwater quality of the shallow coastal aquifer of Eastern Dahomey Basin in southwestern Nigeria. Field physicochemical measurement of EC, pH TDS, Eh, salinity, temperature, and the static water level (SWL) was carried out on 250 shallow wells; 230 water samples were collected for analysis between June 2017 and April 2018. A spatial distribution map of these ions and metals showed an increasing concentration in the dry season water samples compared to those of the wet season. This higher concentration could be attributed to change in the intensity of hydrochemical processes such as evaporation, redox, and mineral precipitation. Results of linear regression modelling established significant relationships between SWL, SO42−, NO3−, Fe, and Eh for both wet and dry seasons with the p-value falling between 75% and 95%, which can also be seen in the plots of Eh/ORP against Fe2+, Mn2+, SO42−, and NO3−. These results revealed the influence of the redox process for both seasons, while also having a higher impact in the dry season while variation of concentration revealed decrease with increase in depth, which could be attributed to a decrease in well hydraulic properties and aeration. An Eh-pH geochemical diagram revealed NO3− as the controlling biogeochemical process over Fe in most of the sample wells. Concentrations of NO3−, Fe, and Mn are above the World Health Organization’s (WHO) standard for drinking water in most water samples. This study has established the link between climate change and groundwater quality in shallow coastal aquifers and suggested the need for strategic groundwater management policy and planning to ameliorate groundwater quality deterioration.
Long-Term Isotope Records of Precipitation in Zagreb, Croatia
Authors: Krajcar Bronić Ines , Barešić Jadranka , Borković Damir , Sironić Andreja , Mikelić Ivanka Lovrenčić , Vreča Polona
Published: January 2020

The isotope composition of precipitation has been monitored in monthly precipitation at Zagreb, Croatia, since 1976. Here, we present a statistical analysis of available long-term isotope data (3H activity concentration, δ2H, δ18O, and deuterium excess) and compare them to basic meteorological data. The aim was to see whether isotope composition reflected observed climate changes in Zagreb: a significant increase in the annual air temperature and larger variations in the precipitation amount. Annual mean δ18O and δ2H values showed an increase of 0.017‰ and 0.14‰ per year, respectively, with larger differences in monthly mean values in the first half of the year than in the second half. Mean annual d-excess remained constant over the whole long-term period, with a tendency for monthly mean d-excess values to decrease in the first half of the year and increase in the second half due to the influence of air masses originating from the eastern Mediterranean. Changes in the stable isotope composition of precipitation thus resembled changes in the temperature, the circulation pattern of air masses, and the precipitation regime. A local meteoric water line was obtained using different regression methods, which did not result in significant differences between nonweighted and precipitation-weighted slope and intercept values. Deviations from the Global Meteoric Water Line GMWL (lower slopes and intercepts) were observed in two recent periods and could be explained by changes in climate parameters. The temperature gradient of δ18O was 0.33‰/°C. The tritium activity concentrations in precipitation showed slight decreases during the last two decades, and the mean A in the most recent period, 2012–2018, was 7.6 ± 0.8 Tritium Units (TU).
Analysis of the Flow in a Typified USBR II Stilling Basin through a Numerical and Physical Modeling Approach
Authors: Macián-Pérez Juan Francisco , García-Bartual Rafael , Huber Boris , Bayon Arnau , Vallés-Morán Francisco José
Published: January 2020

Adaptation of stilling basins to higher discharges than those considered for their design implies deep knowledge of the flow developed in these structures. To this end, the hydraulic jump occurring in a typified United States Bureau of Reclamation Type II (USBR II) stilling basin was analyzed using a numerical and experimental modeling approach. A reduced-scale physical model to conduct an experimental campaign was built and a numerical computational fluid dynamics (CFD) model was prepared to carry out the corresponding simulations. Both models were able to successfully reproduce the case study in terms of hydraulic jump shape, velocity profiles, and pressure distributions. The analysis revealed not only similarities to the flow in classical hydraulic jumps but also the influence of the energy dissipation devices existing in the stilling basin, all in good agreement with bibliographical information, despite some slight differences. Furthermore, the void fraction distribution was analyzed, showing satisfactory performance of the physical model, although the numerical approach presented some limitations to adequately represent the flow aeration mechanisms, which are discussed herein. Overall, the presented modeling approach can be considered as a useful tool to address the analysis of free surface flows occurring in stilling basins.