bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Genomics of Three Hybrid-Pathogen Multidrug-Resistant Escherichia coli Strains Isolated from Healthy Donors’ Feces
Authors: Ortega-Enríquez Judith Z. , Martínez-de la Peña Claudia , Lara-Ochoa Cristina , Rocha-Gracia Rosa del Carmen , Barrios-Villa Edwin , Arenas-Hernández Margarita M. P.
Published: August 2024

The present study shows the genomic characterization of three pathogenic Escherichia coli hybrid strains. All strains were previously characterized as diarrheagenic pathotypes (DEC), obtained from feces. The three sequenced strains have genes that encode adhesins (fimH and iha) and iron uptake systems (iucC and iutA). Antibiotic resistance genes were also found for fluoroquinolone and aminoglycoside families in the three strains. The presence of genomic islands (GIs) in the sequenced study strains presented 100% identity (Ec-25.2) and 99% identity (Ec-36.1) with previously reported Extraintestinal Pathogenic E. coli (ExPEC) strains. The Ec-36.4 strain shared a 99% identity with GI from the Enterotoxigenic E. coli (ETEC) pathotype of the diarrheagenic E. coli strain. Ec-25.2 belongs to ST69 and harbors a FimH27 variant, while Ec-36.1 and Ec-36.4 belong to ST4238 and share a FimH54 variant. Four incompatibility groups associated with conjugative plasmids were identified (IncFIB, IncF11, IncI1, and IncB/O/K/Z), as well as Insertion Sequences and MITEs elements.
Efficient Broad-Spectrum Cyanophage Function Module Mining
Authors: Guo Yujing , Dong Xiaoxiao , Li Huiying , Lin Wei , Cao Lei , Li Dengfeng , Zhang Yiming , Jin Jin , Tong Yigang , Liu Zihe
Published: August 2024

Cyanobacterial harmful algal blooms (CyanoHABs) cause health and environmental effects worldwide. Cyanophage is a virus that exclusively infects cyanobacteria. Using cyanophages to control blooms is the latest biological control method. However, little research on the genomics of cyanophages and the presence of numerous proteins with unidentified functions in cyanophage genomes pose challenges for their practical application and comprehensive investigation. We selected the broad-spectrum and efficient cyanophage YongM for our study. On the one hand, through rational analysis, we analyze essential genes, establish the minimal cyanophage genome and single essential gene modules, and examine the impact of essential modules on growth. Additionally, we conducted ultraviolet mutagenesis on YongM to generate more efficient cyanophages’ critical modules through random mutagenesis. Then, we sequenced and analyzed the functionality of the mutational gene modules. These findings highlight several gene modules that contribute to a deeper understanding of the functional components within cyanophage genomes.
Separation of Microplastics from Blood Samples Using Traveling Surface Acoustic Waves
Authors: Mesquita Pedro , Lin Yang , Gong Liyuan , Schwartz Daniel
Published: August 2024

Microplastics have emerged as ubiquitous contaminants, attracting increasing global attention. Recent evidence confirms the presence of microplastics in human blood, suggesting their potential to interact with cells and induce adverse physiological reactions in various organs as blood circulates. To quantify the distribution of microplastics and assess their potential effects on human health, the effective separation of microplastics from blood is crucial. However, current methods for separating microplastics from blood are limited in effectiveness and simplicity. This study proposes a microfluidic device that utilizes traveling surface acoustic waves to separate microplastics from blood. While traveling surface acoustic waves have been employed to separate various particles, a systematic study on the separation of microplastics from blood samples has not been previously reported. Specifically, the theoretical values of the acoustic radiation factor for various types of microplastics and blood cells were investigated. The significant differences in resonant frequencies indicated the feasibility of separating microplastics of different sizes and types from blood cells. Experimental validation was performed using a polydimethylsiloxane microfluidic device on a piezoelectric lithium niobate substrate. The device successfully separated 5- and 10-micrometer polystyrene microplastics from blood samples. The effects of power and flow rate on separation efficiency were also systematically investigated. This study provides a novel approach for the effective separation of microplastics from blood, contributing to the assessment of their distribution and potential health impacts.
Sexual Dimorphism and Hypothalamic Astrocytes: Focus on Glioprotection
Authors: Thomaz Natalie K. , Bobermin Larissa Daniele , Quincozes-Santos André
Published: August 2024

Sexual dimorphism refers to biological differences between males and females in the same species, including morphological, physiological, and behavioral characteristics. Steroid hormones are associated with changes in several brain regions, as well as the pathophysiology of aging, obesity, and neuropsychiatric diseases. The hypothalamus controls several physiological processes, including metabolism, reproduction, circadian rhythm, and body homeostasis. Refined communication between neurons and glial cells, particularly astrocytes, coordinates physiological and behavioral hypothalamic functions. Therefore, from previously published studies, this review aims to highlight sex-related differences in rodent hypothalamic astrocytes, since we believe that this brain region is essential for the understanding of dimorphic patterns that are influenced by steroid sex hormones. Thus, we review concepts of sexual dimorphism, the hypothalamic-pituitary-gonadal axis, the role of hormonal influence on hypothalamic astrocyte functions, neuroglial communication, as well as sexual dimorphism and neuropsychiatric disorders and glioprotective mechanisms associated with the hypothalamus.
A Practical Guide to Manual and Semi-Automated Neurosurgical Brain Lesion Segmentation
Authors: Jain Raunak , Lee Faith , Luo Nianhe , Hyare Harpreet , Pandit Anand S.
Published: August 2024

The purpose of the article is to provide a practical guide for manual and semi-automated image segmentation of common neurosurgical cranial lesions, namely meningioma, glioblastoma multiforme (GBM) and subarachnoid haemorrhage (SAH), for neurosurgical trainees and researchers. Materials and Methods: The medical images used were sourced from the Medical Image Computing and Computer Assisted Interventions Society (MICCAI) Multimodal Brain Tumour Segmentation Challenge (BRATS) image database and from the local Picture Archival and Communication System (PACS) record with consent. Image pre-processing was carried out using MRIcron software (v1.0.20190902). ITK-SNAP (v3.8.0) was used in this guideline due to its availability and powerful built-in segmentation tools, although others (Seg3D, Freesurfer and 3D Slicer) are available. Quality control was achieved by employing expert segmenters to review. Results: A pipeline was developed to demonstrate the pre-processing and manual and semi-automated segmentation of patient images for each cranial lesion, accompanied by image guidance and video recordings. Three sample segmentations were generated to illustrate potential challenges. Advice and solutions were provided within both text and video. Conclusions: Semi-automated segmentation methods enhance efficiency, increase reproducibility, and are suitable to be incorporated into future clinical practise. However, manual segmentation remains a highly effective technique in specific circumstances and provides initial training sets for the development of more advanced semi- and fully automated segmentation algorithms.
The Impact of the Mediterranean Diet on Telomere Biology: Implications for Disease Management—A Narrative Review
Authors: Baliou Stella , Ioannou Petros , Apetroaei Miruna-Maria , Vakonaki Elena , Fragkiadaki Persefoni , Kirithras Evangelos , Tzatzarakis Manolis N. , Arsene Andreea Letitia , Docea Anca Oana , Tsatsakis Aristides
Published: August 2024

Introduction: Telomeres are nucleoprotein complexes at the ends of chromosomes that are under the control of genetic and environmental triggers. Accelerated telomere shortening is causally implicated in the increasing incidence of diseases. The Mediterranean diet has recently been identified as one that confers protection against diseases. This review aimed to identify the effect of each component of the Mediterranean diet on telomere length dynamics, highlighting the underlying molecular mechanisms. Methods: PubMed was searched to identify relevant studies to extract data for conducting a narrative review. Results: The Mediterranean diet alleviates clinical manifestations in many diseases. Focusing on autoimmune diseases, the Mediterranean diet can be protective by preventing inflammation, mitochondrial malfunction, and abnormal telomerase activity. Also, each Mediterranean diet constituent seems to attenuate aging through the sustenance or elongation of telomere length, providing insights into the underlying molecular mechanisms. Polyphenols, vitamins, minerals, and fatty acids seem to be essential in telomere homeostasis, since they inhibit inflammatory responses, DNA damage, oxidative stress, mitochondrial malfunction, and cell death and induce telomerase activation. Conclusions: The Mediterranean diet is beneficial for maintaining telomere dynamics and alleviating age-related illnesses. This review provides a comprehensive overview of cross-sectional, observational, and randomized controlled trials regarding the beneficial impact of every constituent in the Mediterranean diet on telomere length and chronic disease management.
Identifying Future Study Designs and Indicators for Somatic Health Associated with Diets of Cohorts Living in Eco-Regions: Findings from the INSUM Expert Workshop
Authors: Średnicka-Tober Dominika , Góralska-Walczak Rita , Kopczyńska Klaudia , Kazimierczak Renata , Oczkowski Michał , Strassner Carola , Elsner Friederike , Matthiessen Lea Ellen , Bruun Thea Steenbuch Krabbe , Philippi Rosane Beatriz , Zanasi Cesare , Van Vliet Marja , Dragsted Lars Ove , Husain Sarah , Damsgaard Camilla Trab , Lairon Denis , Kesse-Guyot Emmanuelle , Baudry Julia , Leclercq Catherine , Stefanovic Lilliana , Welch Ailsa , Bügel Susanne Gjedsted
Published: August 2024

Diets, but also overall food environments, comprise a variety of significant factors with direct and indirect impacts on human health. Eco-Regions are geographical areas with a territorial approach to rural development, utilizing organic food and farming practices, and principles and promoting sustainable communities and food systems. However, so far, little attention has been given to quantifying aspects of the health of citizens living in these sustainable transition territories. The project “Indicators for Assessment of Health Effects of Consumption of Sustainable, Organic School Meals in Eco-Regions” (INSUM) aims to identify and discuss research approaches and indicators that could be applied to effectively measure the somatic, mental, and social health dimensions of citizens in Eco-Regions, linked to the intake of organic foods in their diets. In this paper, we focus on the somatic (physical) health dimension. A two-day workshop was held to discuss suitable methodology with an interdisciplinary, international group of experts. The results showed the limitations of commonly used tools for measuring dietary intake (e.g., relying on the memory of participants), and nutritional biomarkers (e.g., variations in correlations with specific intakes) for research understanding dietary intake and the health effects of diets. To investigate the complexity of this issue, the most suitable approach seems to be the combination of traditional markers of physical and mental health alongside emerging indicators such as the microbiome, nutrigenomics, metabolomics, or inflammatory biomarkers. Using new, digital, non-invasive, and wearable technologies to monitor indicators could complement future research. We conclude that future studies should adopt systemic, multidisciplinary approaches by combining not only indicators of somatic and mental health and social wellbeing (MHSW) but also considering the potential benefits of organic diets for health as well as aspects of sustainability connected to food environments.
Unraveling the Interaction between Inflammation and the Cardiometabolic Index in Older Men: A Pilot Study
Authors: Carvalho Rafael L. , Brito Tábatta R. P. , Amaral Jônatas B. , Monteiro Fernanda R. , Lima Daniela B. , Pereira Thalles A. M. , da Costa Beatriz F. , Furtado Guilherme E. , Rodrigues Pamella M. M. , dos Santos Carlos A. F. , Bachi André L. L. , Sarmento Adriana de Oliveira
Published: August 2024

Both cardiometabolic and chronic inflammatory diseases pose a significant challenge to global public health, particularly among older adults. Here, we investigated the interplay between systemic inflammatory status and the cardiometabolic index (CMI) in older men with adequate weight or obesity. In this observational cross-sectional study, older men (71.79 ± 7.35 years) were separated into groups with normal weight (NW, n = 34) and obesity (O, n = 32) to assess circulating levels of pro- and anti-inflammatory cytokines and CMI. Overall, the O group showed not only a higher inflammatory status but also increased CMI (p < 0.0001) compared with the NW group. Interestingly, only positive correlations were found between pro- and anti-inflammatory cytokines in both groups. Through multivariate regression analysis, IL-6 (β = −0.2276, p = 0.0003) and IL-10 (β = 0.2023, p = 0.0030) significantly influenced CMI in the NW group. No significant results were found in the O group. Our findings reinforce the effects of obesity in inflammaging, as well as suggesting that the influence of cytokines in CMI occurs in older men with normal weight, since the elevated pro-inflammatory profile observed in older men with obesity can interfere in this effect.
Impact of Physical Activity on Overall Survival and Liver Cirrhosis Incidence in Steatotic Liver Disease: Insights from a Large Cohort Study Using Inverse Probability of Treatment Weighting
Authors: Yang Keungmo , Chung Beom Sun , Ryu Tom
Published: August 2024

Physical activity is a cornerstone of a healthy lifestyle, with benefits in managing chronic diseases. This study investigates the relationship between physical activity and liver-related outcomes with or without steatotic liver diseases, including metabolic dysfunction-associated steatotic liver disease (MASLD) and MASLD and increased alcohol intake (MetALD). The primary outcomes of interest were overall survival in the entire population, individuals without steatotic liver disease, patients with MASLD, and those with MetALD. The secondary outcomes included the incidence of liver cirrhosis. Participants were categorized based on physical activity frequency and Kaplan–Meier survival curves and Cox proportional hazards models were used for analysis. Higher physical activity was associated with significantly better survival in the overall cohort and MASLD cohort before and after inverse probability of treatment weighting (IPTW). In participants without steatotic liver disease and the MetALD cohort, higher physical activity showed significant survival improvement after IPTW. For the incidence of liver cirrhosis, higher physical activity showed significant associations before IPTW in the overall cohort and MASLD cohort, but these associations were not significant after IPTW. Marginal significance was observed in the MetALD cohort before and after IPTW. In conclusion. promoting physical activity may be key in improving liver-related outcomes.
Identifying Malnutrition Risk in the Elderly: A Single- and Multi-Parameter Approach
Authors: Kujawowicz Karolina , Mirończuk-Chodakowska Iwona , Cyuńczyk Monika , Witkowska Anna Maria
Published: August 2024

Malnutrition is a significant concern affecting the elderly, necessitating a complex assessment. This study aims to deepen the understanding of factors associated with the assessment of malnutrition in the elderly by comparing single- and multi-parameter approaches. In this cross-sectional study, 154 individuals underwent a comprehensive geriatric assessment (CGA). Malnutrition risk was determined using the mini nutritional assessment (MNA). Additional factors assessed included sarcopenia, polypharmacy, depression, appetite, handgrip strength, and gait speed. Phase angle (PA) and body composition were measured using bioelectrical impedance analysis (BIA). The MNA identified a malnutrition risk in 36.8% of individuals. The geriatric depression scale (GDS) and PA demonstrated moderate effectiveness in assessing malnutrition risk, with AUC values of 0.69 (95% CI: 0.60–0.78) and 0.62 (95% CI: 0.54–0.72), respectively. A logistic regression model incorporating handgrip strength, skeletal muscle mass, sarcopenia, osteoporosis, depression, specific antidepressant use, mobility, appetite, and smoking achieved superior performance in predicting malnutrition risk, with an AUC of 0.84 (95% CI: 0.77–0.91). In conclusion, this study demonstrates that integrating multiple parameters into a composite model provides a more accurate and comprehensive assessment of malnutrition risk in elderly adults.
Nursing Process Related to the Nursing Focus “Airway Clearance”: A Scoping Review
Authors: Gaspar Luís , Reis Neuza , Sousa Paula , Silva Abel Paiva e , Cardoso Alexandrina , Brito Alice , Bastos Fernanda , Campos Joana , Parente Paulo , Pereira Filipe , Machado Natália
Published: August 2024

Background: Airway clearance impairment has a significant impact on self-care and quality of life. Identifying clinical data, nursing diagnoses, and nursing interventions is essential to clinical reasoning and enhancing nursing care. This study aims to map the existing evidence on clinical data, nursing diagnoses, and nursing interventions addressing the nursing focus on “airway clearance”. Methods: Research was conducted based on Joanna Briggs’s Scoping Review Methodology. We searched four databases for published studies until December 2023. Results: From the initial 1854 studies identified, 123 were included in the review. The findings highlighted two areas of nursing attention: one related to signs and symptom management, and the other related to education and coping strategies. The data that led to nursing diagnoses were divided into cognitive and clinical data. The nursing diagnoses were mostly related to secretion retention, excessive mucus production, and airway obstruction. The most commonly identified nursing interventions were educational interventions assembled into predesigned education programs rather than patient-tailored programs. Conclusions: Findings can add substantial value for systematizing the nursing process related to “airway clearance”, improving nursing decision-making and care quality. This study was prospectively registered with the Open Science Framework (OSF) on 02 December 2022, with the registration number wx5ze.
Prevalence of Respiratory Pathogens in Nasopharyngeal Swabs of Febrile Patients with or without Respiratory Symptoms in the Niakhar Area of Rural Senegal
Authors: Ndiaye Dame , Diatta Georges , Bassene Hubert , Cortaredona Sébastien , Sambou Masse , Ndiaye Anna Julienne Selbe , Bedotto-Buffet Marielle , Edouard Sophie , Mediannikov Oleg , Sokhna Cheikh , Fenollar Florence
Published: August 2024

Acute respiratory tract infections are one of the leading causes of morbidity and mortality worldwide. More data are needed on circulating respiratory microorganisms in different geographical areas and ecosystems. We analyzed nasopharyngeal swabs from 500 febrile patients living in the Niakhar area (Senegal), using FTDTM multiplex qPCR and simplex qPCR to target a panel of 25 microorganisms. We detected at least one microorganism for 366/500 patients (73.2%), at least one virus for 193/500 (38.6%), and at least one bacterium for 324/500 (64.8%). The most frequently detected microorganisms were Streptococcus pneumoniae (36.8%), Haemophilus influenzae (35.8%), adenovirus (11.8%), influenza viruses (6.4%), rhinovirus (5.0%), SARS-CoV-2 (4.0%), and RSV (4.0%). The main microorganisms significantly associated with respiratory symptoms, with a p-value ≤ 0.05, were influenza virus (11.9% in patients with respiratory symptoms versus 2.9% in patients without), RSV (6.5% versus 2.6%), metapneumovirus (5.4% versus 1.3%), HPIVs (7.6% versus 1.0%), S. pneumoniae (51.9% versus 28.0%), and H. influenzae (54.6% versus 24.5%). Co-infections were significantly associated with respiratory symptoms (65.4% versus 32.9%). All the epidemiological data show a high level of circulation of respiratory pathogens among febrile patients, including those preventable by vaccination such as S. pneumoniae, raising the question of the serotypes currently circulating. Furthermore, the availability of affordable real-time etiological diagnostic tools would enable management to be adapted as effectively as possible.
Morphological and Biochemical Changes in the Mediterranean Cereal Cyst Nematode (Heterodera latipons) during Diapause
Authors: Abumuslem Motasem , Toktay Halil , Sadder Monther T. , Dababat Abdelfattah A. , Salem Nida’ M. , AL-Banna Luma
Published: August 2024

The cereal cyst nematode (Heterodera latipons) is becoming an economically important species in global cereal production as it is being identified in many new cereal cultivated areas and causes significant losses. Consequently, understanding its biology becomes crucial for researchers in identifying its vulnerabilities and implementing effective control measures. In the current study, different morphological and biochemical changes of H. latipons cysts containing eggs with infective juveniles from a barley field in Jordan were studied during the summer of 2021, at two sample dates. The first, at the harvest of the cereal crop (June 2021), when the infective second-stage juveniles (J2s) were initiating diapause, and the second, before planting the sequent cereal crop (late October 2021), when the J2s were ending diapause. The studied population was characterized morphologically and molecularly, showing 98.4% molecular similarity to both JOD from Jordan and Syrian “300” isolates of H. latipons. The obtained results and observations revealed that there were dramatic changes in all the investigated features of the cysts and eggs they contained. Morphological changes such as cyst color, sub-crystalline layer, and thickness of the rigid eggshell wall were observed. A slight change in the emergence time of J2s from cysts was observed without any difference in the number of emerged J2s. The results of biochemical changes showed that the total contents of carbohydrates, glycogen, trehalose, glycerol, and protein were higher in cysts collected in October when compared to those cysts collected in June. The SDS-PAGE pattern indicated the presence of a protein with the size of ca. 100 kDa in both sampling dates, whereas another protein (ca. 20 kDa) was present only in the cysts of October. Furthermore, the expression of trehalase (tre) gene was detected only in H. latipons collected in October. The outcomes of this study provide new helpful information that elucidates diapause in H. latipons and may be used for the implementation of new management strategies of cyst nematodes.
Optimization of Sodium Iodide-Based Root Filling Material for Clinical Applications: Enhancing Physicochemical Properties
Authors: Park Hye-Shin , Kim Yu-Jin , Chang Soo-Jin , Lee Hae-Hyoung , Han Mi-Ran , Lee Joon-Haeng , Kim Jong-Soo , Kim Jong-Bin , Shin Ji-Sun , Lee Jung-Hwan
Published: August 2024

Premature loss of root canal-treated primary teeth has long been a concern in dentistry. To address this, researchers developed a sodium iodide-based root canal-filling material as an alternative to traditional iodoform-based materials. The goal of this study was to improve the physicochemical properties of the sodium iodide-based material to meet clinical use standards. To resolve high solubility issues in the initial formulation, researchers adjusted component ratios and added new ingredients, resulting in a new paste called L5. This study compared L5 with L0 (identical composition minus lanolin) and Vitapex as controls, conducting physicochemical and antibacterial tests. Results showed that L5 met all ISO 6876 standards, demonstrated easier injection and irrigation properties than Vitapex, and exhibited comparable antibacterial efficacy to Vitapex, which is currently used clinically. The researchers conclude that if biological stability is further verified, L5 could potentially be presented as a new option for root canal-filling materials in primary teeth.
Identification of the Biotransformation Pathways of a Potential Oral Male Contraceptive, 11β-Methyl-19-Nortestosterone (11β-MNT) and Its Prodrugs: An In Vitro Study Highlights the Contribution of Polymorphic Intestinal UGT2B17
Authors: Bachhav Namrata , Singh Dilip Kumar , Blithe Diana L. , Lee Min S. , Prasad Bhagwat
Published: August 2024

11β-Methyl-19-nortestosterone dodecylcarbonate (11β-MNTDC) is a prodrug of 11β-MNT and is being considered as a promising male oral contraceptive candidate in clinical development. However, the oral administration of 11β-MNTDC exhibits an ~200-fold lower serum concentration of 11β-MNT compared to 11β-MNTDC, resulting in the poor bioavailability of 11β-MNT. To elucidate the role of the first-pass metabolism of 11β-MNT in its poor bioavailability, we determined the biotransformation products of 11β-MNT and its prodrugs in human in vitro models. 11β-MNT and its two prodrugs 11β-MNTDC and 11β-MNT undecanoate (11β-MNTU) were incubated in cryopreserved human hepatocytes (HHs) and subjected to liquid chromatography–high resolution tandem mass spectrometry analysis, which identified ten 11β-MNT biotransformation products with dehydrogenated and glucuronidation (11β-MNTG) metabolites being the major metabolites. However, 11β-MNTG formation is highly variable and prevalent in human intestinal S9 fractions. A reaction phenotyping study of 11β-MNT using thirteen recombinant UDP-glucuronosyltransferase (UGT) enzymes confirmed the major role of UGT2B17 in 11β-MNTG formation. This was further supported by a strong correlation (R2 > 0.78) between 11β-MNTG and UGT2B17 abundance in human intestinal microsomes, human liver microsomes, and HH systems. These results suggest that 11β-MNT and its prodrugs are rapidly metabolized to 11β-MNTG by the highly polymorphic intestinal UGT2B17, which may explain the poor and variable bioavailability of the drug.
Quenched Zwitterionic Cyclic Arg-Gly-Asp-Containing Pentapeptide Probe for Real-Time Brain Tumor Imaging
Authors: Kim Hyunjin , Liu Maixian , Choi Yongdoo
Published: August 2024

The efficacy of glioblastoma treatment is closely associated with complete tumor resection. However, conventional surgical techniques often result in incomplete removal, leading to poor prognosis. A major challenge is the accurate delineation of tumor margins from healthy tissues. Imaging-guided surgery, particularly using fluorescent probes, is a promising solution for intraoperative guidance. The recently developed ‘always-on’ types of targeted fluorescence probes generate signals irrespective of their presence in tumor cells or in blood circulation, hampering their effectiveness. Here, we propose a novel activatable fluorescence imaging probe, Q-cRGD, that targets glioma cells via the specific binding of the cyclic Arg-Gly Asp-containing pentapeptide (cRGD) to integrins. The Q-cRGD probe was synthesized by conjugating a near-infrared (NIR) dye to a tryptophan quencher via a disulfide linkage, including a cRGD-targeting ligand. This activatable probe remained inactive until the redox-responsive cleavage of the disulfide linkage occurred within the target cell. The zwitterionic nature of NIR dyes minimizes nonspecific interactions with serum proteins, thereby enhancing the tumor-to-background signal ratio (TBR). An in vivo fluorescence imaging study demonstrated a TBR value of 2.65 within 3 h of the intravenous injection of Q-cRGD, confirming its potential utility in imaging-guided brain cancer surgery.
Chitosan-Coated Liposome Formulations for Encapsulation of Ciprofloxacin and Etoposide
Authors: Gil-Gonzalo Rubén , Durante-Salmerón D. Alonzo , Pouri Saeedeh , Doncel-Pérez Ernesto , Alcántara Andrés R. , Aranaz Inmaculada , Acosta Niuris
Published: August 2024

Cancer and bacterial infections rank among the most significant global health threats. accounting for roughly 25 million fatalities each year. This statistic underscores the urgent necessity for developing novel drugs, enhancing current treatments, and implementing systems that boost their bioavailability to achieve superior therapeutic outcomes. Liposomes have been recognised as effective carriers; nonetheless, they encounter issues with long-term stability and structural integrity, which limit their pharmaceutical applicability. Chitosomes (chitosan-coated liposomes) are generally a good alternative to solve these issues. This research aims to demonstrate the effective individual encapsulation of ciprofloxacin (antibacterial, hydrophilic) and etoposide (anticancer, hydrophobic), within chitosomes to create more effective drug delivery systems (oral administration for ciprofloxacin, parenteral administration for etoposide). Thus, liposomes and chitosomes were prepared using the thin-film hydration technique and were characterised through ATR-FTIR, Dynamic Light Scattering (DLS), zeta potential, and release profiling. In both cases, the application of chitosomes enhanced long-term stability in size and surface charge. Chitosome-encapsulated ciprofloxacin formulations exhibited a slower and sustained release profile, while the combined effect of etoposide and chitosan showed heightened efficacy against the glioblastoma cell line U373. Therefore, coating liposomes with chitosan improved the encapsulation system’s properties, resulting in a promising method for drug delivery.
Modulation of Interlayer Nanochannels via the Moderate Heat Treatment of Graphene Oxide Membranes
Authors: Meng Na , Sun Xin , Liu Jinxin , Mi Jialing , Chen Xuan , Rong Rong
Published: August 2024

In response to the phenomenon of interlayer transport channel swelling caused by the hydration of oxygen-containing functional groups on the GO membrane surface, a moderate heat treatment method was employed to controllably reduce the graphene oxide (GO) membrane and prepare a reduced GO composite nanofiltration membrane (mixed cellulose membrane (MCE)/ethylenediamine (EDA)/reduced GO-X (RGO-X)). The associations of different heat treatment temperatures with the hydrophilicity, interlayer structure, permeability and dye/salt rejection properties of GO membranes were systematically explored. The results indicated that the oxygen-containing groups of the GO membrane were partially eliminated after heat treatment, and the hydrophilicity was weakened. This effectively weakened the hydration between the GO membrane and the water molecules and inhibited the swelling of the oxidized graphene membrane. In the dye desalination test, the MCE/EDA/RGO membrane exhibited an ultra-high rejection rate of over 97% for methylene blue (MB) dye molecules. In addition, heat treatment increased the structural defects of the GO membrane and promoted the fast passage of water molecules via the membrane. In pure water flux testing, the water flux of the membrane remained above 46.58 Lm−2h−1bar−1, while the salt rejection rate was relatively low.
Study on Bonding Characteristics of Polymer Grouted Concrete-Soil Interface
Authors: Wang Lina , Yang Xiaodong , Diao Yueliang , Guo Chengchao
Published: August 2024

The issue of interfacial shear damage has been a significant challenge in the field of geotechnical engineering, particularly in the context of diaphragm walls and surrounding soils. Polymer grouting is a more commonly used repair and reinforcement method but its application to interface repair and reinforcement in the field of geotechnical engineering is still relatively rare. Consequently, this paper presents a new polymer grouting material for use in grouting reinforcement at the interface between concrete and soils. The bonding characteristics and shear damage mode of the interface after grouting were investigated by the direct shear test, and the whole process of interface shear damage was investigated by digital image correlation (DIC) technology. Finally, the reinforcement mechanism was analyzed by microscopic analysis. The results demonstrate that the permeable polymer is capable of effectively filling the pores of soil particles and penetrating into the concrete-soil interface. Through a chemical reaction with water in the soil, the polymer cements the soil particles together, forming chemical adhesion at the interface and thereby achieving the desired reinforcement and repair effect. In the shear process, as the normal stress increased, the horizontal displacement and horizontal compressive strain at the distal end of the loading end decreased, while the maximum vertical displacement and maximum vertical strain of the cured soil also decreased. The results of scanning electron microscopy (SEM) demonstrated that the four groups of test polymers exhibited a reduction in soil porosity of 53.47%, 58.79%, 52.71%, and 54.12%, respectively. Additionally, the form of concrete-soil interfacial bonding was observed in the concrete-cohesive layer-cured soil mode. The findings of this study provide a foundation for further research on diaphragm wall repair and reinforcement.
The Incorporation of Sulfonated PAF Enhances the Proton Conductivity of Nafion Membranes at High Temperatures
Authors: Cai Kun , Yu Jinzhu , Tan Wenjun , Gao Cong , Zhao Zili , Yuan Suxin , Cheng Jinghui , Yang Yajie , Yuan Ye
Published: August 2024

Nafion membranes are widely used as proton exchange membranes, but their proton conductivity deteriorates in high-temperature environments due to the loss of water molecules. To address this challenge, we propose the utilization of porous aromatic frameworks (PAFs) as a potential solution. PAFs exhibit remarkable characteristics, such as a high specific surface area and porosity, and their porous channels can be post-synthesized. Here, a novel approach was employed to synthesize a PAF material, designated as PAF-45D, which exhibits a specific surface area of 1571.9 m2·g−1 and possesses the added benefits of facile synthesis and a low cost. Subsequently, sulfonation treatment was applied to PAF-45D in order to introduce sulfonic acid groups into its pores, resulting in the formation of PAF-45DS. The successful incorporation of sulfonic groups was confirmed through TG, IR, and EDS analyses. Furthermore, a novel Nafion composite membrane was prepared by incorporating PAF-45DS. The Nyquist plot of the composite membranes demonstrates that the sulfonated PAF-45DS material can enhance the proton conductivity of Nafion membranes at high temperatures. Specifically, under identical film formation conditions, doping with a 4% mass fraction of PAF-45DS, the conductivity of the Nafion composite membrane increased remarkably from 2.25 × 10−3 S·cm−1 to 5.67 × 10−3 S·cm−1, nearly 2.5 times higher. Such promising and cost-effective materials could be envisioned for application in the field of Nafion composite membranes.
Hybridization of Polymer-Encapsulated MoS2-ZnO Nanostructures as Organic–Inorganic Polymer Films for Sonocatalytic-Induced Dye Degradation
Authors: Palanisamy Gowthami , Bhosale Mrunal , Magdum Sahil S. , Thangarasu Sadhasivam , Oh Tae-Hwan
Published: August 2024

The development of environmentally friendly technology is vital to effectively address the issues related to environmental deterioration. This work integrates ZnO-decorated MoS2 (MZ) to create a high-performing PVDF-based PVDF/MoS2-ZnO (PMZ) hybrid polymer composite film for sonocatalytic organic pollutant degradation. An efficient synergistic combination of MZ was identified by altering the ratio, and its influence on PVDF was assessed using diverse structural, morphological, and sonocatalytic performances. The PMZ film demonstrated very effective sonocatalytic characteristics by degrading rhodamine B (RhB) dye with a degradation efficiency of 97.23%, whereas PVDF only degraded 17.7%. Combining MoS2 and ZnO reduces electron–hole recombination and increases the sonocatalytic degradation performance. Moreover, an ideal piezoelectric PVDF polymer with MZ enhances polarization to improve redox processes and dye degradation, ultimately increasing the degradation efficiency. The degradation efficiency of RhB was seen to decrease while employing isopropanol (IPA) and p-benzoquinone (BQ) due to the presence of reactive oxygen species. This suggests that the active species •O2− and •OH are primarily responsible for the degradation of RhB utilizing PMZ2 film. The PMZ film exhibited improved reusability without substantially decreasing its catalytic activity. The superior embellishment of ZnO onto MoS2 and effective integration of MZ into the PVDF polymer film results in improved degrading performance.
Economic Dispatch of Integrated Energy Systems Considering Wind–Photovoltaic Uncertainty and Efficient Utilization of Electrolyzer Thermal Energy
Authors: Li Ji , Xu Lei , Zhang Yuying , Kou Yang , Liang Weile , Bieerke Alihan , Yuan Zhi
Published: August 2024

Currently, high levels of output stochasticity in renewable energy and inefficient electrolyzer operation plague IESs when combined with hydrogen energy. To address the aforementioned issues, an IGDT-based economic scheduling strategy for integrated energy systems is put forth. Firstly, this strategy establishes an IES consisting of coupled electricity, heat, hydrogen, and gas taking the hydrogen production electrolyzer’s thermal energy utilization into account. Second, to minimize the system’s overall operating costs, a deterministic scheduling model of the IES is built by taking into account the stepped carbon trading mechanism and the integrated demand response. Lastly, an optimal dispatch model is built using the information gap decision theory under the two strategies of risk aversion and risk seeking, taking into account the uncertainty of renewable energy generation. CPLEX is the solver used to solve the proposed model. After taking into account the effective use of thermal energy from the electrolyzer and loads demand response, the results show that the system carbon emission is reduced by 2597.68 kg and the operating cost is lowered by 44.65%. The IES scheduling model based on IGDT can effectively manage costs while maintaining system risk control, all while accommodating decision-makers’ varying risk preferences. This study can provide a useful reference for the research related to the scheduling of the IES low-carbon economy.
Reverse Logistics and the Circular Economy: A Study before and after the Implementation of the National Solid Waste Policy in Brazil
Authors: Aguirre Rodríguez Elias Carlos , Hernández Cecilia Toledo , Aguirre-Rodríguez Elen Yanina , da Silva Aneirson Francisco , Marins Fernando Augusto Silva
Published: August 2024

This article analyzes Reverse Logistics (RL) practices associated with the concept of Circular Economy (CE), comparing studies conducted before and after the implementation of the National Solid Waste Policy (NSWP) in Brazil. Data from companies in the Sul Fluminense region were analyzed using the Analytic Hierarchy Process to prioritize RL practices and identify those related to CE. The research included companies from nine Brazilian economic sectors that engage in some form of RL. The comparison of results aimed to evaluate the influence of the NSWP on these practices, with data analyzed using the Super Decisions software (version 3.2) to establish priorities and aggregate them using the Aggregating Individual Priorities method. It was observed that the implementation of the NSWP in 2010 emphasized the importance of sustainability. Although the concept of CE is recent, its operationalization is based on consolidated RL practices such as recycling, remanufacturing, and material reuse. The study provides insights to optimize and expand these practices, facilitating the transition from a linear economy to a circular one.
The Glories of Scripturally Informed Natural Law in Secular Education
Authors: Wenkel David H.
Published: August 2024

Today’s culture is becoming increasingly secularized and characterized by social fragmentation. Christian teachers in public schools should no longer expect to share the same worldview as their students. It is only natural to ask the question: can anything good come about in a public-school classroom? This essay outlines a Christian vision for individual educators in public and pluralistic contexts that draws from a scripturally informed view of natural law. This paper answers this question in the affirmative: not only can it be good, but it can also be glorious, even if such glories are diminutive.
A Comprehensive Heritage BIM Methodology for Digital Modelling and Conservation of Built Heritage: Application to Ghiqa Historical Market, Saudi Arabia
Authors: Baik Ahmad
Published: August 2024

Preserving historical architectural structures is crucial for safeguarding cultural heritage. This study explores the application of Heritage Building Information Modelling (HBIM) to enhance the documentation process of the Ghiqa Historical Market in Saudi Arabia, a monument known for its intricate architecture and cultural significance. Traditional documentation methods often fail to capture detailed features accurately and rely on labour-intensive manual processes. HBIM uses advanced digital technologies to improve precision, efficiency, and preservation efforts. In this study, point cloud data from 3D laser scanning is used to create a detailed digital model of the market, covering structural systems, material attributes, architectural features, and historical context. The research also integrates historical archives and photographs to enrich the model with additional contextual information. This comprehensive approach provides a holistic understanding of the Ghiqa Historical Market, aiding accurate preservation and restoration decisions. HBIM offers several advantages in architectural documentation. The digital model enhances visualization, allowing stakeholders to explore the site from multiple perspectives. It also serves as a tool for analysing structural integrity, identifying potential risks, and planning restoration interventions. Moreover, digital documentation ensures effective knowledge transfer across generations, preserving valuable architectural heritage for future reference and research. Additionally, it promotes interdisciplinary collaboration among architects, historians, conservators, and other stakeholders involved in preservation. Including the Ghiqa Historical Market in the UNESCO World Heritage List would highlight its global significance, attracting international attention and resources for its preservation. This designation would underscore the market’s cultural and historical importance, fostering a sense of pride and responsibility in its maintenance. The implementation of Heritage BIM demonstrates its potential to revolutionize heritage conservation by combining diverse data sources into a single, detailed, and accessible digital resource.
Semi-Arid to Arid Scenario Shift: Is the Cabrobó Desertification Nucleus Becoming Arid?
Authors: da Silva José Lucas Pereira , da Silva Junior Francisco Bento , de Souza Santos João Pedro Alves , dos Santos Almeida Alexsandro Claudio , da Silva Thieres George Freire , Oliveira-Júnior José Francisco de , Araújo Júnior George do Nascimento , Scheibel Christopher Horvath , da Silva Jhon Lennon Bezerra , de Lima João Luís Mendes Pedroso , da Silva Marcos Vinícius
Published: August 2024

Monitoring areas susceptible to desertification contributes to the strategic development of regions located in environments of extreme hydric and social vulnerability. Therefore, the objective of this study is to evaluate the process of soil degradation in the Desertification Nucleus of Cabrobó (DNC) over the past three decades using remote sensing techniques. This study used primary climatic data from TerraClimate, geospatial data of land use and land cover (LULC), and vegetation indices (SAVI and LAI) via Google Earth Engine (GEE) from Landsat 5/TM and 8/OLI satellites, and established the aridity index (AI) from 1992 to 2022. The results indicated 10 predominant LULC classes with native vegetation suppression, particularly in agriculture and urbanization. SAVI ranged from −0.84 to 0.90, with high values influenced by La Niña episodes and increased rainfall; conversely, El Niño episodes worsened the rainfall regime in the DNC region. Based on the Standardized Precipitation Index (SPI), it was possible to correlate normal and severe drought events in the DNC with years under the influence of El Niño and La Niña phases. In summary, the AI images indicated that the DNC remained semi-arid and that the transition to an arid region is a cyclical and low-frequency phenomenon, occurring in specific periods and directly influenced by El Niño and La Niña phenomena. The Mann–Kendall analysis showed no increasing trend in AI, with a Tau of −0.01 and a p-value of 0.97. During the analyzed period, there was an increase in Non-Vegetated Areas, which showed a growing trend with a Tau of 0.42 in the Mann–Kendall analysis, representing exposed soil areas. Annual meteorological conditions remained within the climatic pattern of the region, with annual averages of precipitation and actual evapotranspiration (ETa) close to 450 mm and an average temperature of 24 °C, showing changes only during El Niño and La Niña events, and did not show significant increasing or decreasing trends in the Mann–Kendall analysis.
Strip Adjustment of Multi-Temporal LiDAR Data—A Case Study at the Pielach River
Authors: Wimmer Michael H. , Mandlburger Gottfried , Ressl Camillo , Pfeifer Norbert
Published: August 2024

With LiDAR (Light Detection and Ranging) time series being used for various applications, the optimal realization of a common geodetic datum over many epochs is a highly important prerequisite with a direct impact on the accuracy and reliability of derived measures. In our work, we develop and define several approaches to the adjustment of multi-temporal LiDAR data in a given software framework. These approaches, ranging from pragmatic to more rigorous solutions, are applied to an 8-year time series with 21 individual epochs. The analysis of the respective results suggests that a sequence of bi-temporal adjustments of each individual epoch and a designated reference epoch brings the best results while being more flexible and computationally viable than the most extensive approach of using all epochs in one single multi-temporal adjustment. With a combination of sparse control patches measured in the field and one selected reference block, the negative impacts of changing surfaces on orientation quality are more effectively avoided than in any other approach. We obtain relative discrepancies in the range of 1–2 cm between epoch-wise DSMs for the complete time series and mean offsets from independent checkpoints in the range of 3–5 cm. Based on our findings, we formulate design criteria for setting up and adjusting future time series with the proposed method.