bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversidad de diatomeas en un sistema fluvial andino: los nutrientes y la conductividad como factores de explicación
Authors: Donato-Rondón Jhon Ch.
Published: November 2020

Se utilizaron comunidades de algas bénticas que crecieron durante cuatro semanas en sustratos artificiales para estudiar la estructura de las comunidades de diatomeas y se midieron los cambios de la diversidad durante nueve (9) muestreos mensuales entre octubre de 2007 y enero de 2009 en un tramo de 50 m en el sector medio del río Tota (Boyacá, Colombia), a 2.540 m de altitud. Se determinaron taxonómicamente las especies de diatomeas de la comunidad béntica, se calcularon los índices de diversidad de Shannon-Wiener [H´] y de Simpson [S] y se evaluaron los cambios durante el muestreo. Así mismo, se midieron las variables hidrológicas y ambientales. La diversidad de especies se incrementó significativamente (p=≤0,05) con las variaciones de fosfatos (P-PO4 3-), de nitratos (N-NO3-) y de flujo (L S-1), lo que sugiere que estos factores locales moldearon la diversidad y la estructura de las diatomeas en el tramo. En el análisis de redundancia (redundancy analysis, RDA) se evidenció que la conductividad (28 %), los fosfatos (27,5 %), el caudal (11,4 %) y los nitratos (8,0 %) contribuyeron a la varianza. Melosira varians, Navicula rynchocephala y Navicula capitatoradiata se asociaron con altas concentraciones de fosfatos, en tanto que Achnanthidium minutissimum y Nitzschia sp2, con concentraciones bajas. Las concentraciones altas de nitratos y el caudal se asociaron con Eunotia minor, Nitzschia sp1 y Reimeria sinuata, en tanto que Epithemia sorex y Gomphonema parvulum, con aguas de alta conductividad y caudales bajos. Los ríos andinos están inmersos en una red que tiene diversos patrones ambientales y físicos y, en ese contexto, el conocimiento de la biodiversidad es la base para la ordenación ambiental de las cuencas y la elaboración de planes de manejo regional de conservación de la biodiversidad.
Estadios ninfales de Anacroneuria spp. (Plecoptera: Perlidae) y su relación con variables fisicoquímicas en tres microcuencas del río Combeima (Ibagué, Colombia).
Authors: Váquiro Capera Carlos
Published: November 2020

El orden Plecoptera representa uno de los grupos de insectos más relevantes en bioindicación en Colombia. En los últimos años su conocimiento ha aumentado ampliamente; no obstante, en algunas zonas de la región andina, los estudios taxonómicos y ecológicos son aún incipientes. Dada la importancia de los plecópteros en quebradas de alta montaña, se evaluó la relación entre la presencia y abundancia de ninfas del género Anacroneuria con variables fisicoquímicas y bacteriológicas en tres microcuencas andinas asociadas con el río Combeima (Tolima, Colombia): Las Perlas, La Plata y Cay. Se realizaron seis colectas diurnas con red Surber en diferentes sustratos (arena, grava-guijarro, roca y hojarasca), durante un ciclo hidrológico (2016-2017; épocas de altas lluvias, bajas lluvias y transiciones). Se registraron 750 organismos, de los cuales, por morfometría tradicional se determinaron dos morfoespecies de Anacroneuria (Asp1 y Asp2). Se encontró correlación negativa entre la abundancia de Asp1 con el fósforo (rs= -0,5266) en la quebrada Las Perlas; conductividad eléctrica (rs= -0,4725) en la quebrada La Plata y nitratos (rs= -0,4821) en la quebrada Cay, mientras que la Asp2 la presentó en DQO (rs= -0,5634) y correlación positiva con temperatura (rs= 0,6750), pH (rs= 0,7079, nitratos (rs= 0,5569) y fosfatos (rs= 0,6511) en la quebrada Las Perlas.
Determinación del espectro de energía de un haz de rayos X terapéutico de kilovoltaje a partir de su curva de atenuación
Authors: Gonçalves Ana Carolina , Wilches Visbal Jorge Homero , Martins Da Costa Alessandro
Published: November 2020

Muchas áreas científicas y tecnológicas asociadas a procesos industriales, radiodiagnóstico y terapia radiante demandan altos niveles de precisión y practicidad en la medición de espectros de energía de rayos X. El espectro de un haz de rayos X puede determinarse de manera directa, a través de un detector de alta resolución, o de manera indirecta utilizando su curva de atenuación y una apropiada técnica matemática. La medición directa del espectro de rayos X suele ser costosa, problemática y exige considerable experticia. El propósito del presente trabajo fue determinar el espectro de energía de un haz de rayos X de kilovoltaje (kV) utilizando una medición indirecta del mismo, basada en la curva de atenuación del haz y en el uso de transformadas de Laplace. Para esto, i) se llevó a cabo una descripción completa y exhaustiva de la derivación matemática de la función que representa al espectro de energía; ii) se reconstruyó el espectro usando el algoritmo multistart junto con la función lsqnonlin de MATLAB; iii) se validó la reconstrucción del espectro comparando los valores de la capa semirreductora del espectro y de la curva de atenuación. Los resultados mostraron que los valores de las capas semirreductoras del espectro y la curva de atenuación fueron próximos entre sí y que el tiempo de cálculo del espectro fue notablemente corto. Por tanto, se concluye que la determinación indirecta del espectro de rayos X a partir de su curva de atenuación y mediante el uso novedoso de la sinergia multistart-lsqnonlin, es una alternativa más simple, más rápida y tan eficaz como la de medición directa del espectro.
The effect of aminoglycosides on horizontal gene transfer in Klebsiella pneumoniae
Authors: Acosta Iván Camilo , Posada Leonardo , Huertas Mónica Gabriela , Zambrano Eder María Mercedes
Published: November 2020

Antibiotic-resistant bacteria represent a global risk to public health. Horizontal gene transfer, a common mechanism for genetic exchange in bacteria, plays an essential role in the acquisition of resistance genes. In this work, we evaluated the effect of sub-lethal concentrations of antibiotics on plasmid transfer by conjugation and transformation in the opportunistic pathogen Klebsiella pneumoniae. Despite not being naturally competent, this bacterium could acquire extracellular DNA from various plasmids at a very low frequency, which increased upon incubating cells with the aminoglycoside antibiotics amikacin and gentamicin. Transfer by conjugation analyzed using a clinical isolate carrying plasmid pNDM-1 also increased in the presence of sub-lethal concentrations of antibiotics. An RNAseq analysis showed differential expression of several genes when cells were incubated in the presence of sub-lethal concentrations of amikacin suggesting metabolic and regulatory changes, as well as alteration of cell envelope components that could affect the uptake of foreign DNA. These results suggest that sub-lethal concentrations of some aminoglycosides, in particular amikacin, can promote the transfer of resistance-bearing genetic elements in K. pneumoniae, which is relevant for understanding the spread of resistance determinants in this human pathogen.
An Effect Analysis Model for Corporate Marketing Mix Based on Artificial Neural Network
Authors: Wang Haiping
Published: November 2020

Under the boom of market economy in China, marketing mix is being updated constantly to facilitate the all-round development of various industries. To accurate evaluate the effect of corporate marketing mix, this paper designs an effect analysis model for corporate marketing mix based on artificial neural network (ANN). Firstly, a complete evaluation index system (EIS) was created for corporate marketing mix, and the weight of each index was assigned through analytic hierarchy process (AHP). Then, a marketing mix of big marketing + service marketing was proposed, and subject to fuzzy comprehensive evaluation (FCE). Finally, an FCE model was constructed for the effect of corporate marketing mix, based on three-layer backpropagation neural network (BPNN). The effectiveness of the model was verified through experiments. The research findings provide a reference for the application of ANN in other fields of effect analysis.
Number of Pixel Change Rate and Unified Average Changing Intensity for Sensitivity Analysis of Encrypted inSAR Interferogram
Authors: Saidi Riad , Cherrid Nada , Bentahar Tarek , Mayache Hicham , Bentahar Atef
Published: November 2020

The transmission of images from satellites to earth is on the brink of many threats which can affect the confidentiality of the data as well as its quality. Several encryption algorithms are used to secure the transmitted images. The objective in this work is to analyze the sensitivity of a particular type of satellite image, which is an interferogram from interferometric imaging systems inSAR system. This image is encrypted by cryptosystem based on the Advanced Encryption Standard with key length of 256 bits (AES-256) standard and the asymmetric Rivest, Shamir & Adelman (RSA) encryption algorithm using Counter-mode encryption (CTR) mode and Output FeedBack (OFB) mode. The analysis made in this paper is carried out on two types of sensitivity. The first analysis is the sensitivity to change of a pixel in the original interferogram and the second is the sensitivity to the key. Two parameters are used to assess sensitivity: The Number of Pixel Change Rate (NPCR) and the Unified Average Changing Intensity (UACI). The obtained results show that the two modes AES-256-OFB and AES-256-CTR are favorable but cannot be implemented on board a satellite without providing a mechanism capable of compensating for the low resistance to error propagation. Metrics on the clear and encrypted interferogram are exploited such as the Structural Similarity Index (SSIM), Gradient-based Structural Similarity (GSSIM), The use of these metrics, allowed us to see that a change of one pixel in the interferogram and the change of the encryption key will affect the quality of the interferogram, as well as a statistical histogram analysis.
Recombinant Sort: N-Dimensional Cartesian Spaced Algorithm Designed from Synergetic Combination of Hashing, Bucket, Counting and Radix Sort
Authors: Kumar Peeyush , Gangal Ayushe , Kumari Sunita
Published: November 2020

Sorting is an essential operation which is widely used and is fundamental to some very basic day to day utilities like searches, databases, social networks and much more. Optimizing this basic operation in terms of complexity as well as efficiency is cardinal. Optimization is achieved with respect to space and time complexities of the algorithm. In this paper, a novel left-field N-dimensional cartesian spaced sorting method is proposed by combining the best characteristics of bucket sort, counting sort and radix sort, in addition to employing hashing and dynamic programming for making the method more efficient. Comparison between the proposed sorting method and various existing sorting methods like bubble sort, insertion sort, selection sort, merge sort, heap sort, counting sort, bucket sort, etc., has also been performed. The time complexity of the proposed model is estimated to be linear i.e.
Tracking and Extracting Action Trajectory of Athlete Based on Hierarchical Features
Authors: Pan Tao
Published: November 2020

The feature extraction from athlete action images is a research hotspot. To accurately evaluate athlete actions, it is necessary to partition the original image into refined blocks, and extract different levels of image features. However, the traditional feature extraction algorithms can only roughly divide action images into several classes, failing to acquire the accurate feature sets of the actions. This leads to relatively poor performance of feature extraction from action images. To overcome the defect of the traditional methods, this paper puts forward a feature extraction method for the action images of badminton players based on hierarchical features. The underlying image features were analyzed based on the techniques of badminton players, and mapped to the feature space of the corresponding dimension. Simulation results show that the proposed method can accurately extract the features from athlete action images.
Object Detection Using Stacked YOLOv3
Authors: Padmanabula Sai Shilpa , Puvvada Ramya Chowdary , Sistla Venkatramaphanikumar , Kolli Venkata Krishna Kishore
Published: November 2020

Object detection is a stimulating task in the applications of computer vision. It is gaining a lot of attention in many real-time applications such as detection of number plates of suspect cars, identifying trespassers under surveillance areas, detecting unmasked faces in security gates during the COVID-19 period, etc. Region-based Convolution Neural Networks(R-CNN), You only Look once (YOLO) based CNNs, etc., comes under Deep Learning approaches. In this proposed work, an improved stacked Yolov3 model is designed for the detection of objects by bounding boxes. Hyperparameters are tuned to get optimum performance. The proposed model evaluated using the COCO dataset, and the performance is better than other existing object detection models. Anchor boxes are used for overlapping objects. After removing all the predicted bounding boxes that have a low detection probability, bounding boxes with the highest detection probability are selected and eliminated all the bounding boxes whose Intersection Over Union value is higher than 0.4. Non-Maximal Suppression (NMS) is used to only keep the best bounding box. In this experimentation, we have tried with various range of values, but finally got better result at threshold 0.5.
Analysis on the Current Situation and Optimization Strategy of Statistics Teaching in China
Authors: Shang Yuncheng
Published: November 2020

As one of the important teaching subjects, the development and implementation of statistics teaching is conducive to the further realization of the training and construction of China’s statistical talents, and has an important role in promoting China’s social development and economic prosperity. Based on this, people from all walks of life have recently paid more and more attention to the training of statistics talents, thus effectively promoting the development and implementation of statistics teaching reform in China. In this article, the development of statistics teaching in colleges and universities in China is deeply explored and analyzed. During this process, the main shortcomings in statistics teaching in China are explored in detail, and corresponding optimization strategies are formulated, aiming at further improving and optimizing the quality of statistics teaching.
Plantas medicinais utilizadas na comunidade de Campo Preto, Arneiroz, Ceará
Authors: Brito Selma Freire de , Evangelista Antônio Wanderson Lopes
Published: November 2020

A etnobotânica tem o papel de ajudar na compreensão das relações entre o homem e as plantas de uma comunidade. O objetivo desta pesquisa foi realizar um levantamento sobre as plantas medicinais utilizadas na comunidade de Campo Preto, Armeiroz, Ceará, localizada em uma região de Caatinga. Os dados foram obtidos através de uma entrevista previamente preparada que buscava identificar o perfil das pessoas que usam; quais as plantas medicinais utilizadas; as formas de preparo; as partes consumidas; onde essas plantas são obtidas; e para quais doenças são empregadas. Sobre os entrevistados a maioria possui ensino fundamental incompleto e aprendeu sobre plantas medicinais com alguém da família. Foram relatadas 19 plantas, sendo a mais citada Lippia alba. A comunidade recorre ao uso de plantas principalmente para combater resfriados, sendo preparado principalmente na forma de chá. Chama atenção a alta quantidade de espécies exóticas usadas pela população local. Por fim, sabe-se que as plantas medicinais são consumidas por todas as famílias entrevistadas, ressaltando-se a necessidade de conhecer e de incentivar o uso de espécies da flora nativa.
Assessment of Hip Joint Spacer for Treating Patients with Periprosthetic Infection
Authors:   Voronezh State Medical University named after N.N. Burdenko, Varfolomeev D.I. , Samoday V.G. ,   Voronezh State Medical University named after N.N. Burdenko
Published: November 2020

Цель. Оценка возможности использования разработанного спейсера при лечении инфекционных осложнений после операции эндопротезирования тазобедренного сустава. Материал и методы. Для улучшения результатов лечения парапротезной инфекции разработан спейсер тазобедренного сустава. Он состоит из головки, шейки и ножки, изготовленных из костного цемента. Внутри него имеется каркас, соединенный с помповым микронасосом. Каркас выполнен в виде четырех металлических трубок, внутри которых находятся растворы лекарственных средств. Поршни насосов изготовлены из ферромагнита для возможности взаимодействия с магнитом, подносимым к поверхности тела. В спейсере имеется возможность выделения из него лекарственных средств в послеоперационном периоде посредством воздействия на него внешнего магнитного поля. На базе кафедры травматологии и ортопедии Воронежского государственного медицинского университета им. Н.Н. Бурденко был создан действующий макет спейсера. Для оценки функциональных возможностей спейсера были проведены экспериментальные исследования на кроликах. В брюшную полость животным под внутривенным наркозом устанавливали имплантат, содержащий раствор Пирогенала. Проводили оценку средней температуры в исследуемой группе до начала эксперимента и после введения Пирогенала (под воздействием магнитного поля). Результаты. Температура после начала работы спейсера достоверно отличалась от температуры до начала эксперимента (t-критерий – 17,17, p<0,05), что можно объяснить попаданием в кровь животных Пирогенала, содержащегося в спейсере. Проведенные исследования продемонстрировали возможность выделения лекарственных средств, находящихся внутри спейсера, во внутреннюю среду под воздействием на спейсер магнитного поля. Заключение. Предложенный имплантат позволяет увеличить объем и количество лекарственных средств, выделяемых из него в рану. При этом у хирургов есть возможность после операции неинвазивно контролировать скорость введения препаратов в парапротезное пространство для обеспечения их необходимой концентрации. Научная новизна статьи впервые разработан спейсер тазобедренного сустава, позволяющий под воздействием внешнего магнитного поля выделять в парапротезное пространство лекарственные средства, находящиеся внутри самого спейсера. По сравнению с другими способами введения спейсер увеличивает количество лекарственных средств, поступающих непосредственно в околопротезные ткани, что, соответственно, снижает их возможные побочные эффекты.
Peripheral Neurodestruction in Patients with Local Cold Injury
Authors:   Chita State Medical Academy, Mikhailichenko M.I. , Shapovalov K.G. ,   Chita State Medical Academy, Figurski S.A. ,   Chita State Medical Academy, Mudrov V.A. ,   Chita State Medical Academy, Mikhailichenko S.I. ,   Chita State Medical Academy
Published: November 2020

Цель. Определить уровень периферического нейрофиламента Н у пациентов в разные сроки криоповреждения, оценить изменения биоэлектрических потенциалов мышц у пострадавших. Материал и методы. В исследование включено 30 человек. Пострадавшие находились на стационарном лечении в краевом центре термической травмы на базе городской больницы №1 г. Читы с местной холодовой травмой дистальных сегментов стоп в период с 2018 по 2019 год. Измерение биоэлектрической активности выполнялось на 5-е и 30-е сутки от момента получения травмы. Для оценки биоэлектрической активности мышц использован метод накожной электронейромиографии. Концентрацию периферического нейрофиламента н в сыворотке крови определяли методом иФа с использованием реагентов фирмы «Вектор-Бест» на 5-е и 30-е сутки с момента криоповреждения. Результаты. на 5-е сутки с момента криотравмы в исследуемой группе уровень периферического нейрофиламента был выше относительно показателей контроля. Амплитуда М-ответа, резидуальная латентность и скорость распространения возбуждения достоверно были ниже относительно контроля. На 30-е сутки отмечено снижение уровня периферического нейрофиламента по сравнению с более ранним периодом, но значение по-прежнему остается выше показателей контроля. При этом исследуемые показатели электронейромиографии возросли по сравнению с данными в ранние сроки криотравмы, но по-прежнему были ниже группы контроля. Выявлено увеличение нейромышечной активности у пострадавших относительно более раннего периода травмы, что указывает на восстановление нервной проводимости. Заключение. у пострадавших с отморожениями II-IV степени в крови выявлено резкое повышение фосфорилированного периферического нейрофиламента в разные периоды криоповреждения. У пациентов в разные сроки местной холодовой травмы II-IV степени отмечается снижение биоэлектрической активности мышц. На 30-е сутки комплексного лечения отмечается увеличение биоэлектрической активности мышц и снижение уровня нейрофиламента Н относительно более раннего периода. Научная новизна статьи впервые изучено содержание периферического нейрофиламента н у пациентов с местной холодовой травмой в разные сроки криоповреждения. Впервые исследована биоэлектрическая активность мышц у пациентов с отморожениями. Установлено, что показатель биоэлектрической активности мышц и уровень содержания периферического нейрофиламента н у пациентов с местной холодовой травмой имеют обратную корреляционную связь.
Features of Endothelial Dysfunction in the Development of Hemorrhagic Complications in Patients with Urolithiasis in the Postoperative Period
Authors:   Voino-Yasenetsky Krasnoyarsk State Medical University, Dunaevskaya S.S. , Vinnik Yu.S. ,   Voino-Yasenetsky Krasnoyarsk State Medical University, Berezhnoi A.G. ,   Voino-Yasenetsky Krasnoyarsk State Medical University
Published: November 2020

Цель. Выявить особенности эндотелиальной дисфункции при развитии геморрагических осложнений в послеоперационном периоде у пациентов с уролитиазом. Материал и методы. Проведено исследование 174 пациентов с мочекаменной болезнью. Пациенты были разделены на две клинические группы: I группа – 93 пациента с неосложненным течением послеоперационного периода; II группа – 81 пациент, у которых развились геморрагические осложнения. В предоперационном периоде и на 3-и сутки после операции проводили оценку экспрессии CD38 и CD31 на лимфоцитах; подсчет количества мембран-высвобожденных микрочастиц, а также оценивали блеббинг лимфоцитов периферической крови. Результаты. В предоперационном периоде было зарегистрировано увеличение экспрессии лимфоцитами CD38 и CD31, более выраженное во II клинической группе. У пациентов I клинической группы количество лимфоцитов в состоянии начального и терминального блеббинга было в пределах средних значений группы контроля. Во II клинической группе отмечалось значимое увеличение данных показателей. количество свободных микрочастиц лимфоцитарного происхождения в периферической крови пациентов было статистически увеличено во II клинической группе и составляло 874 [643; 1095]. При анализе данных, полученных в послеоперационном периоде, было выявлено статистически значимое повышение экспрессии лимфоцитами CD38 и CD31, преобладающее во II клинической группе, количество лимфоцитов в состоянии начального блеббинга было увеличено (7,61 и 9,26 соответственно). Показатель блеббингирующих лимфоцитов в терминальном состоянии был увеличен в обеих клинических группах (8,55 и 19,17 соответственно). Заключение. Таким образом, у пациентов с уролитиазом в послеоперационном периоде развивается эндотелиальная недостаточность. Однако, при развитии геморрагических осложнений механизм и выраженность межклеточной коммуникации в патогенезе формирования дисфункции эндотелия существенно отличаются, что необходимо учитывать при проведении оперативного пособия и назначении гемостатической терапии в раннем послеоперационном периоде. Научная новизна статьи впервые изучены особенности развития эндотелиальной дисфункции при развитии геморрагических осложнений в раннем послеоперационном периоде у пациентов с уролитиазом. Установлено, что в послеоперационном периоде происходит повышение экспрессии лимфоцитами CD38 и CD31, возрастает количество лимфоцитов в терминальном состоянии и свободных микрочастиц лимфоцитарного происхождения в периферической крови, данные изменения преобладают в группе пациентов с геморрагическими осложнениями.
Features of Platelet Aggregation in Patients with COVID-19 Infection. Preliminary results
Authors:   Mogilev Regional Clinical Hospital, Marochkov А.V. ,   Vitebsk State Medical University, Lipnitski A.L. ,   Mogilev Regional Clinical Hospital,   Vitebsk State Medical University, Tsopau D.S. ,   Mogilev Hospital No1, Kupreyeva I.A. ,   Mogilev Regional Clinical Hospital, Dazortsava V.U. ,   Mogilev Regional Clinical Hospital
Published: November 2020

Цель. Определить изменения агрегации тромбоцитов при интенсивной терапии пациентов с инфекцией COVID-19, находящихся в критическом состоянии. Материал и методы. Проведено пилотное нерандомизированное проспективное клиническое исследование агрегации тромбоцитов у пациентов, которые поступали в отделения анестезиологии и реанимации сострым респираторным дистресс – синдромом с диагнозом COVID-19 (группа 1, n=15). Также была сформирована группа сравнения (группа 2, n=15), в которой у практически здоровых людей амбулаторно проводили исследование агрегации тромбоцитов. Забор крови в группе 1 проводился в 1-3-и сутки пребывания пациентов в палате интенсивной терапии. Исследование проводилось на анализаторе агрегации тромбоцитов АР 2110 (ЗАО «СОЛАР», Республика Беларусь). Результаты. При индукции агрегации тромбоцитов высокими дозами АДФ (1,25 и 2,5 мкг/мл) у пациентов с COVID-19 наблюдалась более низкая степень агрегации (58,7 (34,2; 63,2) % и 66,5 (59,6; 70,9) % в сравнении с 67,2 (64,1; 70,3) % и 77,5 (71,8; 80,3) % в группе 2 соответственно (р<0,05)), которая происходила существенно быстрее (161,5 (103; 214) с и 197 (127; 309) с в сравнении с 413 (325; 465) с и 362,5 (296,5; 422) с в группе 2 соответственно (р<0,05)). С индуктором адреналином в дозах 2,5 и 5 мкМ степень агрегации также была достоверно ниже у пациентов с COVID-19 (40,8 (26,1; 63,9) % и 38,7 (29,4; 66,8)% соответственно). C индуктором коллагеном у пациентов с инфекцией COVID-19 наблюдалась однофазная необратимая агрегация тромбоцитов со степенью агрегации 75,2 (71,9; 83,2) %. Заключение. У пациентов с COVID-19 степень агрегации тромбоцитов с АДФ и адреналином была значительно снижена, что связано с назначением им антикоагулянтной и антиагрегантной терапии (в связи с основной и сопутствующей патологией). Научная новизна статьи Впервые было проведено исследование агрегации тромбоцитов у пациентов с инфекцией COVID-19, находящихся в критическом состоянии. Установлено, что степень агрегации тромбоцитов с АДФ и адреналином была значительно снижена в связи с проведением антикоагулянтной и антиагрегантной терапии данным пациентам. С индуктором коллагеном наблюдалась однофазная необратимая агрегация тромбоцитов со степенью агрегации 75,2 (71,9; 83,2) %.
Experience in the Treatment of Late Mechanical Suture Failure after Longitudinal Laparoscopic Sleeve Gastrectomy Using Minimally Invasive Techniques
Authors:   E.A.Vagner Perm State Medical University, Kotelnikova L.P. ,   Perm Regional Clinical Hospitall, Fedachuk A.N. ,   Perm Regional Clinical Hospitall, Shvarev A.V. ,   Perm Regional Clinical Hospitall
Published: November 2020

последнее десятилетие лапароскопическая продольная резекция желудка заняла ведущую позицию среди бариатрических операций. К наиболее тяжелым осложнениям послеоперационного периода относят несостоятельность механического шва культи желудка. Целью данного сообщения служит демонстрация случая успешного лечения поздней несостоятельности механического шва после лапароскопической продольной резекции желудка с использованием мини-инвазивных методик. Пациентке, 56 лет, с индексом массы тела 50, выполнена лапароскопическая продольная резекция желудка с использованием калибровочного зонда 36 Fr. Проверка на герметичность линии механического шва проведена введением 150 мл жидкости, окрашенной метиленовым синим. Несостоятельность механического шва была диагностирована на 12 сутки после операции с помощью компьютерной томографии. При рентгенологическом исследовании желудка, проведенном дважды, обнаружить затеков контрастного вещества не удалось. С целью закрытия зоны несостоятельности культи желудка эндоскопическим путем был установлен полностью покрытый саморасширяющийся стент, а зона абсцесса была дренирована при релапароскопии. При дислокации стента ниже кардиального жома проведена его переустановка. Через 4 недели свищ перестал функционировать,
Case Report of Giant Nonmetastatic Gastrointestinal Solid Tumor without Clinical Manifestations in a Middle-Aged Male Patient
Authors:   Sumy State University, Vynnychenko I. , Kononenko M. ,   Sumy State University, Smorodska O. ,   Sumy State University,   Utility Non-Profit Enterprise Clinical Hospital No5 of Sumy Municipal Council, Ivanov S. ,   Utility Non-Profit Enterprise Clinical Hospital No5 of Sumy Municipal Council, Moskalenko Y. ,   Sumy State University, Vynnychenko O. ,   Sumy State University, Sikora V. ,   Sumy State University, Lyndin M. ,   Sumy State University, Romaniuk A. ,   Sumy State University
Published: November 2020

Gastrointestinal stromal tumors (GIST) are one of the most common mesenchymal tumors that originate from the intestinal cells of Cajal. The most common localization of GIST is the gastrointestinal tract, however, extragastrointestinal forms are sometimes found. Most GISTs are small in size, which most often does not exceed 5 - 8 cm in maximum dimension. This article describes the case of a patient with a non-metastatic giant tumor of the stomach without any clinical manifestations. The tumor was localized in the abdominal cavity, occupying almost all of it, squeezing and displacing the surrounding organs. Due to the cystic-solid structure, its gigantic size and intimate adherence to the surrounding organs, it was not possible to carry out an accurate differential diagnosis before surgery. Intraoperative imaging of the tumor, further histological and immunohistochemical (CD117, CD34, S100, CD45, PanCK; Ki-67) studies made possible to establish the diagnosis of gastric GIST T4N0M0 stage II. Based on the data obtained, despite the gigantic size, the prognosis of the disease was considered favorable (low mitotic index, no metastasis). During the period of clinical observation, within 1.5 years after surgery, no relapse or disease progression was recorded, which indicates a favorable prognosis for the patient.
Primary health care policy and vision for community pharmacy and pharmacists in Colombia
Authors: Amariles Pedro , Ceballos Mauricio , Gonzalez-Giraldo Cesar
Published: November 2020

Colombia is a decentralized republic with a population of 50 million, constituted by 32 departments (territorial units) and 1,204 municipalities. The health system provides universal coverage and equal access to health care services to 95% of the population. Primary health care is seen as a practical approach that guarantees the health and well-being of whole-of-society. The National Pharmaceutical Policy (NPP, 2012) goal is "to develop strategies that enable the Colombian population equitable access to effective medicines, through quality pharmaceutical services (PS)”. There are 4,351 providers certified to deliver PS: 3,699 (85%) ambulatory and 652 (15%) hospital care. The goals for PS are: a) promoting healthy lifestyles; b) preventing risk factors arising from medication errors; c) promoting rational use of medicines; and d) implementing Pharmaceutical Care. There are a number of ways that ambulatory patients access medications: through intermediary private companies, public and private hospitals pharmacies, and retail establishments (drugstores and pharmacies). Intermediary private companies are similar to Pharmaceutical Benefits Management in the U.S. health system, and act as intermediaries between health insurers, pharmaceutical laboratories, and patients. Pharmacists are being employed by these companies and in health insurance companies managing, auditing and delivering rational use of medicines programs. In 2014 there were approximately 20,000 pharmacies and drugstores, (private establishments) where a significant number of prescription-only medicines are sold without medical prescription. Colombian laws allow personal without pharmacy education to be a “director” in these establishments, so the training and education of persons working in drugstores and pharmacies is an important challenge. There about 8,000 registered pharmaceutical chemists with 25% to 30% working in patient care. Since the 90´s, there are more favorable conditions for pharmacist’s participation and contribution to health system and patient’s health outcome. These environmental facilitators include: a) laws and regulations regarding pharmaceutical services (2005-2007), b) establishment of a NPP (2012), and c) opportunities associated with the consolidation of private health management companies providing health services with an interest in pharmaceutical services (since 1995). Finally, telepharmacy, comprehensive care routes for pharmaceutical services, and further strengthen of postgraduate training in pharmacy practice are future strategies to improve the pharmacy profession in Colombia. They provide an opportunity to influence the recognition and value of the pharmacist as the health care professional.
Upravljanje vzdrževanja
Authors: Drstvenšek Igor , Leber Marjan
Published: November 2020

Učbenik opisuje funkcije vzdrževanja, namenjene ugotavljanju storilnosti in zagotavljanju učinkovitosti proizvodnega procesa. Za razumevanje proizvodne učinkovitosti so najprej pojasnjeni pojmi učinkovitosti, zanesljivosti in vzdrževalnosti proizvodne opreme in njihov vpliv na stroške vzdrževanja. V nadaljevanju sta podrobneje pojasnjeni metodi preventivnega in vzdrževanja na osnovi zanesljivosti. Celostno produktivno vzdrževanje je koncept, ki je uspel združiti vse dobre prakse, ki so se razvile skozi razvoj preventivnega vzdrževanja, vzdrževanja na osnovi zanesljivosti, celostnega upravljanja kakovosti (TQM) in tako imenovanih vitkih načel proizvodnje. Predstavljeni so vidiki uvajanja celostnega produktivnega vzdrževanja, s poudarkom na metodah, ki so se uspešno izkazale v zahodnem svetu. Med njimi je najpomembnejše merjenje skupne učinkovitosti, ki pokaže velikost proizvodnih izgub. Metode 5S, SMED in 20 ključev, opisane v nadaljevanju služijo odpravljanju osnovnih izgub v podjetju in s tem višanju faktorja skupne učinkovitosti opreme.
Improving Production and Productivity of Chickpea (Cicer arietinum L.) Through Scaling-up of Improved Technologies in the Vertisol Highland Areas of Amhara Region, Ethiopia
Authors: Kassa Yehuala , Mamo Dejene , Abie Amsalu , Tigabe Abiro , Ayele Teklemariam , Kefelegn Nigussie , Teferra Beneberu
Published: November 2020

Background: Chickpea seed supply is limited for a local level farmer to the farmer seed exchange systems. The scale-up was conducted in the vertisol potential areas of North Shewa Zone of Amhara Region to create wider demand for improved chickpea varieties, strengthen the linkage among the possible actors and enhancing technology multiplication and dissemination to improve its productivity.
Methods: Naatolii, Mastewal, Arerti and Habru improved chickpea varieties were introduced and more than 393ha of land was covered and about 826 farmers were accessed through delivering more than 49.3 tons of improved seed.
Result: The productivity of chickpea in the area was boosted to 2.61tons ha-1 and yield advantage of 73% to 97.7% was obtained from improved varieties compared to the local cultivar. As a result of this intervention, many farmers benefited easy access to improved seeds of different chickpea varieties through direct seed marketing and farmer to farmer seed exchange.
Prevalence of Nematodes in Rhizosphere of Kiwi Plants (Actinidia deliciosa Chev., 1984) in International Centre for Integrated Mountain Development (ICIMOD), Knowledge Park, Godawari, Lalitpur, Nepal
Authors: Chhetri B. , Subedi J. R.
Published: November 2020

Background: Plants harbor many trophic groups of nematodes in them. The plant production is also determined by the occurrence of nematodes adjacent to the rhizosphere of plants, such as parasitic, free-living etc.
Methods: Altogether 60 samples (30 from 20 cm depth and 30 from 30 cm depth) were examined from the Kiwi plants to detect the nematode distribution in Kiwi plants in ICIMOD, Godawari, Lalitpur, Nepal. Overall, 1510 individuals of 10 nematodes genera (both free living/beneficial and parasitic) belonging to four orders were found to be associated with Kiwi plants, among them the highest report was of order Mononchida (44.04%) which was followed by Rhabditida (33.44%), Dorylaimida (17.88%) and Tylenchida (4.64%). The number of nematodes were higher at 20 cm depth than at 30 cm depth. Iotonchus spp. was highest (32.68%) and the number of Helichotylenchus spp. (3.93%) and Mesorhabditis spp. (3.92) were lowest at 20 cm depth. Similarly, Iotonchus spp. was highest (20.13%) and the number of Discolaimus spp. (4.03%) was lowest at 30 cm depth.
Extent of Adoption of “Novel Organic Liquid Nutrients” in Fruits and Vegetable Crops
Authors: Modi P. K. , Chavan S. M. , Verma P. D.
Published: November 2020

Background: Novel organic liquid Nutrients have been developed based on banana pseudostem comprising of 10 macro and micro nutrients and 2 hormones, which are naturally synthesized. It is developed by Navsari Agricultural University, Navsari in Year 2009-10 under management and value addition of banana pseudostem (NAIP-II) project. To know the extent of adoption of Novel organic liquid nutrients in fruit and vegetable crops, a study was formulated to know the correlation of different factors and characteristics for adoption of the product.
Methods: This study was conducted in the Vyara, Valod, Songadh and Dolvan blocks of Tapi district of Gujarat through Krishi Vigyan Kendra, NAU, Vyara, Tapi during 2018-19. In the study, the sample size was of 100 respondent were selected through stratified random sampling method and determine the correlation of dependent and independent variable on knowledge and adoption of Novel organic liquid nutrients in fruits and vegetable crops.
Result: In this study revealed that the adoption of Novel organic liquid nutrients up to 59 per cent in fruit crops and 76 per cent in vegetables crops among farmers. The data on correlation for extent of adoption of Novel organic liquid nutrients in fruits and vegetable crops were showed significantly results with education, training and knowledge at 5 per cent (0.1965) level of probability.
Investigation of the Effects of Micromeria congesta Essential Oil Extract on Wound Healing in Rabbits and Molecular Genetics Applications
Authors: Yavuz U. , Dinc H. , Yigin A. , Yumusak N. , Aslan M. , Yoldas A. , Ozel A. , Mýzraklýdag A. S.
Published: November 2020

Background: The chemical composition and antimicrobial and antioxidant properties of Micromeria congesta (M. congesta) have been previously revealed, but their effect on wound healing has not been investigated. This study aimed to investigate the effects of M. congesta extract in rabbits where full-thickness skin wounds were used.
Methods: Twentyone New Zealand rabbits that were used in the study were divided into 3 groups as Group I (M. congesta’s essential oil extract), Group II (Centella asiatica (C. asiatica) extract in pomade form) and Group III (control). Two wounds of 2.8 cm in size were created at the caudal border of the scapula. While the unhealed wound areas were measured on the 7th, 14th, 21st and 28th days.
Result: It was observed histopathologically and immunohistochemically that the healing was more noticeable in Group II in comparison to the others and the wound healing in Group I was more limited. In the genetic analysis, the expression levels of all the target genes in Group I and Group II were significantly higher than those in the control group on the 7th day (p less than 0.05). The results demonstrate that topical application of the oily form of M. congesta results in significant improvements on wound healing in rabbits.
Molecular Identification and Characterization of Chicken Parvovirus from Indian Chicken and Association with Runting and Stunting Syndrome
Authors: Pradeep M. , Reddy M. R. , Kannaki T. R.
Published: November 2020

Background: Runting-stunting syndrome (RSS) in chickens is a worldwide problem, attributed with several etiological agents. The present study aimed to identify the association of enteric viruses with RSS in different chicken flocks.
Methods: Intestinal samples from 14 flocks of chicken of different age and breed and with or without RSS were collected randomly from necropsy samples, isolated nucleic acids, and screened for major enteric viruses by Polymerised chain reaction (PCR), using species-specific primers.
Result: Chicken Parvovirus (ChPV) was identified in 100% of the flocks with RSS, in two of which ChPV alone was detected. While in others it was associated with Avian nephritis virus, Avian Rotavirus, Chicken astrovirus, and Fowl adenovirus in 80%, 50%, 30% and 10% flocks, respectively. RSS was reproduced and isolated ChPV by chicken embryo inoculation using the samples from ChPV alone infected cases. Sequence analysis of ChPV revealed closer association with Ecuodor isolates than the Asian isolates. The results indicated the presence of ChPV in Indian chicken flocks and its close association with RSS.
Studies on Development and Antigen Expression of Biofilm Cells of Vibrio anguillarum for Oral Vaccination in Aquaculture
Authors: Ramesh K. S. , Poojary Sathish Rama , Kumar B.T. Naveen , Patil Prakash , Abhiman P. B. , Shankar K. M. , Patel Deepti Manjari
Published: November 2020

Background: Vibrio anguillarum is one of the major pathogen causing economic loss in the aquaculture industry. And, most of the microorganisms will use biofilm strategy for their survival in the host. Hence, less effectiveness of antibiotic and free cell (whole cell) vaccine may be observed in the aquaculture practice. Therefore the developed vaccine should be the mirror image of pathogen molecule, in regard; the present study was carried out to standardize the nutrient requirement for optimum biofilm production and antigen expression of biofilm and free cells of Vibrio anguillarum. Also, to identify the deferentially expressed protein in the biofilm mode.
Methods: Culture conditions were optimized for the maximum biofilm production of V. anguillarum on chitin flakes as a substrate in nutrient restricted conditions. Through proteomic approach such as SDS PAGE was conducted to know the protein profile of biofilm and free cells of V. anguillarum and further, nanoLC-MS/MS was used to identify the differentially expressed protein in the biofilm of V. anguillarum.
Result: Maximum biofilm production was observed on the 3rd day on 0.1% TSB concentration supplemented with 0.3% chitin flakes and 2% NaCl. Significant changes in the antigen expression of biofilm of V. anguillarum were observed in SDS PAGE and it revealed that in the biofilm mode, three new novel proteins were expressed and about ten proteins repressed as compared to that of free cell counterpart.
Epidemiological and Clinical Studies in Cats with Thoracolumbar Vertebral Fracture and Luxation and Its Management
Authors: Nath I. , Dhanalakshmi S. , Das M. R. , Panda S. K. , Kundu A. K. , Mishra U. K. , Parvathamma P. S.
Published: November 2020

Background: Thoraco-lumbar spinal trauma frequently results in neurological dysfunction of varying degrees in domestic cats. The consequences may be permanent disability or death depending on severity and segment of spinal cord affected. Assessment of primary damage to spinal cord is important to prevent secondary damage and complications arising from neurological deficit. Since assessment of neurological dysfunction and its treatment in spinal trauma in cats is an evolving field, the present study was undertaken with an objective to record and associate epidemiological data and clinical examination findings at the time of presentation with clinical outcome on 60th post-treatment day in cats with thoracolumbar vertebral fracture and luxation, for a better clinical approach in this condition.
Methods: 24 affected cats were assessed epidemiologically and clinically at the time of presentation. All the cats were treated either conservatively or surgically based on extent of neurological dysfunction and severity of injury on radiographic evaluation. Post-treatment observations was then correlated with epidemiological data and clinical findings to assess the clinical outcome.
Result: In the present study, sub-adult semi-domicile cats were most affected and major cause was automobile accident. Presence of concomitant injuries (30%) affected recovery. Thoracolumbar spinal cord segment (T3-L3) was most affected (71%). Among 16 cats with unstable vertebral fractures, 5/6 cats recovered after surgical treatment and 2/10 cats recovered after conservative treatment. Survival and recovery was poor when degree vertebral canal displacement was more than 70% (10 cats). All 7 cats with grade 1 or grade 2 and one cat with grade 3 neurological dysfunction recovered after conservative treatment. 5 cats with grade 3 and 2 cats with grade 4 neurological dysfunction recovered after surgical treatment. Selection of surgical treatment provided better recovery in cats with unstable vertebral fractures and greater degree of neurological dysfunction.
Optimization of Perilla, Roselle and Zanthoxylum Levels in Pork Patties by Application of Conjoint Analysis
Authors: . Rongsensusang , Rao V. Appa , Babu R. Narendra , Karunakaran R. , Dorai R. Palani , Pandian A. Serma Saravana
Published: November 2020

Background: Perilla, roselle and zanthoxylum are spices which are commonly used in preparation of meat dishes. They provide unique and distinctive flavour and taste which improves the sensory properties of the products to which they are added. In addition, they also possess desirable functional properties like antioxidant and antimicrobial properties of natural origin. The objective of the study was to optimize the inclusion levels in combination with the most acceptable sensory quality and to study the storage stability of pork patties at refrigerated (4±1oC) temperature.
Methods: Three different levels of perilla 0.5, 0.7 and 0.9%, roselle 0.2, 0.3 and 0.4% and zanthoxylum 0.1, 0.15 and 0.2% were used in nine (9) different combinations designated as C1, C2, C3, C4, C5, C6, C7, C8, and C9 in the preparation of pork patties, to determine the most preferred combination according to physicochemical and sensory analysis. The patties were analyzed for pH, emulsion stability, colour and sensory evaluation. Sensory quality of patties was conducted and rating score for each profile, contribution of each attribute to the respondent’s preference was calculated by using conjoint analysis procedure.
Result: The results of conjoint analysis revealed that the combination with perilla 0.5%, roselle 0.2% and zanthoxylum 0.1% was the most preferred combination as compared to all other combinations. Treated patties show lower oxidative and microbial parameters values and higher sensory scores over a period of 21 days.