bluuebunny/crossref_metadata_2025 huggingface dataset:
Carbon Transfer Efficiency and Risk of Fisheries Collapse in Three Large Marine Ecosystems Around China
Authors: Chen Dongxing , Wang Xutao , Hou Minchi , Wang Qiabin , Liu Qianqian , Huang He , Zhang Yafeng
Published: June 2022

Fisheries catch is determined by a complex combination of biological and industrial factors. In this study, using data from the online database Sea Around Us from 1950 to 2018, the risk of fisheries collapse was assessed for the three large marine ecosystems (LMEs) around China by analyzing the carbon transfer efficiency, mean trophic level, and mean maximum length of fisheries catch and expansion factor. In addition, these were compared with the corresponding values for other LMEs, especially the Humboldt Current and the North Sea LMEs, which experienced fisheries collapse.

Our results revealed high carbon transfer efficiencies in LMEs around China, suggesting large fishing efforts compared with LMEs with similar primary production. Although marine fish landings did not decline significantly, they were maintained by potential resources associated with offshore and deep expansion and fishing of lower–trophic-level species and juvenile fish. However, the potential resources have been largely consumed in the East China Sea and South China Sea LMEs, where the ratio of the primary production required to sustain catches to the total primary production (%PPR) was greater than 50%. In contrast, this ratio in the Yellow Sea LME was lower; however, this value was still higher than the sustainable ratio in the Humboldt Current LME. Without proper fisheries management, the three fisheries around China are likely to collapse, as observed in case of the North Sea LME in the 1970s.
Carbon and Lipid Contents of the Copepod Calanus finmarchicus Entering Diapause in the Fram Strait and Their Contribution to the Boreal and Arctic Lipid Pump
Authors: Tarling Geraint A. , Belcher Anna , Blackwell Mayleen , Castellani Claudia , Cook Kathryn Barbara , Cottier Finlo R. , Dewar-Fowler Victoria , Freer Jennifer J. , Gerrish Laura , Johnson Magnus L. , Last Kim S. , Lindeque Penelope Kate , Mayor Daniel J. , Parry Helen E. , Stowasser Gabriele , Wootton Marianne
Published: June 2022

The boreal copepod Calanus finmarchicus sequesters substantial amounts of carbon (C) in the deep layers of the North Atlantic Ocean through their contribution to the “lipid pump.” This pump is driven by these zooplankton descending from the surface layers to spend prolonged periods at depth during which time they metabolise substantial lipid reserves and a fraction suffer mortality. C. finmarchicus is principally a boreal species but is expatriated by currents flowing northwards into Arctic regions such as the Fram Strait, where it is now able to complete its life cycle. We considered how this expansion to its distributional range adds to the estimated magnitude of the lipid pump. Field sampling in the Fram Strait found C. finmarchicus abundance to be spatially variable with high values, equivalent to those reported for core distributional areas further south, found mainly in the eastern region. Lipid reserve levels were sufficient for many individuals to survive the overwintering period and reproduce the following spring. In accordance with abundance patterns, lipid pump magnitude was greater in the Eastern Fram Strait (2.04 g C m−2 year−1) compared to the Western Fram Strait (0.33 g C m−2 year−1). At least for the eastern region, these rates are similar to those reported for this species elsewhere (average of 4.35 g C m−2 year−1). When extrapolated to the wider spatial area of the Fram Strait, the lipid pump generated by this species in this ocean sector amounts to 0.3 Mt C year−1. Although constituting a modest proportion of the total C. finmarchicus lipid pump of 19.3 Mt C year−1, it indicates that the continued northwards expansion of this species will act to increase the size of its lipid pump, which may counteract that lost through the northwards retreat of its Arctic congeners, Calanus glacialis and Calanus hyperboreus.
Evaluating Clinical Educators' Competence in an East Asian Context: Who Values What?
Authors: Jenq Chang-Chyi , Ou Liang-Shiou , Tseng Hsu-Min , Chao Ya-Ping , Lin Jiun-Ren , Monrouxe Lynn V.
Published: June 2022

Background

How to evaluate clinical educators is an important question in faculty development. The issue of who are best placed to evaluate their performance is also critical. However, the whos and the hows of clinical educator evaluation may differ culturally. This study aims to understand what comprises suitable evaluation criteria, alongside who is best placed to undertake the evaluation of clinical educators in medicine within an East Asian culture: specifically Taiwan.

Methods

An 84-item web-based questionnaire was created based on a literature review and medical educational experts' opinions focusing on potential raters (i.e., who) and domains (i.e., what) for evaluating clinical educators. Using purposive sampling, we sent 500 questionnaires to clinical educators, residents, Post-Graduate Year Trainees (PGYs), Year-4~6/Year-7 medical students (M4~6/M7) and nurses.

Results

We received 258 respondents with 52% response rate. All groups, except nurses, chose “teaching ability” as the most important domain. This contrasts with research from Western contexts that highlights role modeling, leadership and enthusiasm. The clinical educators and nurses have the same choices of the top five items in the “personal qualities” domain, but different choices in “assessment ability” and “curriculum planning” domains. The best fit rater groups for evaluating clinical educators were educators themselves and PGYs.

Conclusions

There may well be specific suitable domains and populations for evaluating clinical educators' competence in East Asian culture contexts. Further research in these contexts is required to examine the reach of these findings.
Cardiovascular Biomarkers for Prediction of in-hospital and 1-Year Post-discharge Mortality in Patients With COVID-19 Pneumonia
Authors: Motloch Lukas J. , Jirak Peter , Gareeva Diana , Davtyan Paruir , Gumerov Ruslan , Lakman Irina , Tataurov Aleksandr , Zulkarneev Rustem , Kabirov Ildar , Cai Benzhi , Valeev Bairas , Pavlov Valentin , Kopp Kristen , Hoppe Uta C. , Lichtenauer Michael , Fiedler Lukas , Pistulli Rudin , Zagidullin Naufal
Published: June 2022

Aims

While COVID-19 affects the cardiovascular system, the potential clinical impact of cardiovascular biomarkers on predicting outcomes in COVID-19 patients is still unknown. Therefore, to investigate this issue we analyzed the prognostic potential of cardiac biomarkers on in-hospital and long-term post-discharge mortality of patients with COVID-19 pneumonia.

Methods

Serum soluble ST2, VCAM-1, and hs-TnI were evaluated upon admission in 280 consecutive patients hospitalized with COVID-19-associated pneumonia in a single, tertiary care center. Patient clinical and laboratory characteristics and the concentration of biomarkers were correlated with in-hospital [Hospital stay: 11 days (10; 14)] and post-discharge all-cause mortality at 1 year follow-up [FU: 354 days (342; 361)].

Results

11 patients died while hospitalized for COVID-19 (3.9%), and 11 patients died during the 1-year post-discharge follow-up period (n = 11, 4.1%). Using multivariate analysis, VCAM-1 was shown to predict mortality during the hospital period (HR 1.081, CI 95% 1.035;1.129, p = 0.017), but not ST2 or hs-TnI. In contrast, during one-year FU post hospital discharge, ST2 (HR 1.006, 95% CI 1.002;1.009, p < 0.001) and hs-TnI (HR 1.362, 95% CI 1.050;1.766, p = 0.024) predicted mortality, although not VCAM-1.

Conclusion

In patients hospitalized with Covid-19 pneumonia, elevated levels of VCAM-1 at admission were associated with in-hospital mortality, while ST2 and hs-TnI might predict post-discharge mortality in long term follow-up.
Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts
Authors: Wang Hufei , Li Zhi , Ou Suwen , Song Yanni , Luo Kangjia , Guan Zilong , Zhao Lei , Huang Rui , Yu Shan
Published: June 2022

Despite immune checkpoint blockade (ICB) therapy contributed to significant advances in cancer therapy, only a small percentage of patients with colorectal cancer (CRC) respond to it. Identification of these patients will facilitate ICB application in CRC. In this study, we integrated multiple CRC cohorts (2,078 samples) to construct tumor microenvironment (TME) subtypes using TME indices calculated by CIBERSORT and ESTIMATE algorithms. Furthermore, a surrogate quantitative indicator, a tumor microenvironment immune gene (TMEIG) score system, was established using the key immune genes between TME clusters 1 and 2. The subsequent analysis demonstrated that TME subtypes and the TMEIG score system correlated with clinical outcomes of patients in multiple CRC cohorts and exhibited distinct immune statuses. Furthermore, Tumor Immune Dysfunction and Exclusion (TIDE) analysis indicated that patients with low TMEIG scores were more likely to benefit from ICB therapy. A study on two ICB cohorts (GSE78220 and IMvigor210) also validated that patients with low TMEIG scores exhibited higher ICB response rates and better prognoses after ICB treatment. The biomarker evaluation module on the TIDE website revealed that the TMEIG score was a robust predictive biomarker. Moreover, differential expression analysis, immunohistochemistry, qPCR experiments, and gene set prioritization module on the TIDE website demonstrated that the five genes that constitute the TMEIG score system (SERPINE1, FABP4, SCG2, CALB2, and HOXC6) were closely associated with tumorigenesis, immune cells, and ICB response indices. Finally, TMEIG scores could accurately predict the prognosis and ICB response of patients with CRC. SERPINE1, FABP4, SCG2, CALB2, and HOXC6 might be potential targets related to ICB treatment. Furthermore, our study provided new insights into precision ICB therapy in CRC.
Comprehensive Analysis Reveals USP45 as a Novel Putative Oncogene in Pan-Cancer
Authors: Li Kai , Wang Qian , Bian Hua , Chen Zhiguo , He Haifa , Zhao Xulin , Gong Pengju
Published: June 2022

Background: Deubiquitinating enzymes specifically removes ubiquitin molecules from ubiquitin-tagged target proteins, thereby inhibiting the degradation of target proteins and playing an important role in tumor. However, the mechanism of deubiquitinating enzyme USP45 in tumors remains unclear.

Methods: Based on the RNA-seq data of tissues and cell lines in The Cancer Genome Atlas (TCGA) database, GTEx and CCLE database, the pan-cancer analysis of USP45 expression and survival outcome were performed using R software and Kaplan-Meier Plotter. The structural variants, gene mutations and gene copy number alteration of USP45 were analyzed using the TCGA Pan-Cancer Atlas Studies dataset in the cBioPortal database. The relationships between USP45 and mRNA methylation, tumor heterogeneity, tumor stemness, and tumor immunity were performed by Sangerbox platform and TIMER2.0 using Pearson correlation analysis. Through the ENCORI database and string database, we constructed the ceRNA regulatory mechanism and protein-protein interaction network for USP45. Based on the RNA-seq data in TCGA and GTEx databases, we also constructed the downstream regulatory network for USP45 using the Limma and ClusterProfiler packages of R software. At last, the protein expression levels of USP45 were detected by immunohistochemistry in tumor tissue microarrays.

Results: USP45 is upregulated in most types of tumors and negatively correlated with the overall survival and recurrence-free survival of patient. Furthermore, the structural variation, gene mutations and gene copy number variation of USP45 were identified in different types of tumors. The pan-cancer analysis showed that USP45 was closely related to mRNA methylation, tumor heterogeneity and tumor stemness. In most types of tumors, the expression of USP45 was positively correlated with many immune checkpoint molecules and immune regulators such as PD-L1, while negatively correlated with the infiltration levels of NK cells, Th1 cells, macrophages, and dendritic cells in the tumor microenvironment. Finally, we constructed the ceRNA regulatory network, protein-protein interaction network and downstream regulatory network for USP45 in different types of tumors.

Conclusion: Our study firstly explored the putative oncogenic role of USP45 in pan-cancer, and provided insights for further investigation of USP45.
Evaluation of Clove Phytochemicals as Potential Antiviral Drug Candidates Targeting SARS-CoV-2 Main Protease: Computational Docking, Molecular Dynamics Simulation, and Pharmacokinetic Profiling
Authors: Chandra Manivannan Arun , Malaisamy Arunkumar , Eswaran Murugesh , Meyyazhagan Arun , Arumugam Vijaya Anand , Rengasamy Kannan R. R. , Balasubramanian Balamuralikrishnan , Liu Wen-Chao
Published: June 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus can cause a sudden respiratory disease spreading with a high mortality rate arising with unknown mechanisms. Still, there is no proper treatment available to overcome the disease, which urges the research community and pharmaceutical industries to screen a novel therapeutic intervention to combat the current pandemic. This current study exploits the natural phytochemicals obtained from clove, a traditional natural therapeutic that comprises important bioactive compounds used for targeting the main protease of SARS-CoV-2. As a result, inhibition of viral replication effectively procures by targeting the main protease, which is responsible for the viral replication inside the host. Pharmacokinetic studies were evaluated for the property of drug likeliness. A total of 53 bioactives were subjected to the study, and four among them, namely, eugenie, syzyginin B, eugenol, and casuarictin, showed potential binding properties against the target SARS-CoV-2 main protease. The resultant best bioactive was compared with the commercially available standard drugs. Furthermore, validation of respective compounds with a comprehensive molecular dynamics simulation was performed using Schrödinger software. To further validate the bioactive phytochemicals and delimit the screening process of potential drugs against coronavirus disease 2019, in vitro and in vivo clinical studies are needed to prove their efficacy.
Shared Control of Bimanual Robotic Limbs With a Brain-Machine Interface for Self-Feeding
Authors: Handelman David A. , Osborn Luke E. , Thomas Tessy M. , Badger Andrew R. , Thompson Margaret , Nickl Robert W. , Anaya Manuel A. , Wormley Jared M. , Cantarero Gabriela L. , McMullen David , Crone Nathan E. , Wester Brock , Celnik Pablo A. , Fifer Matthew S. , Tenore Francesco V.
Published: June 2022

Advances in intelligent robotic systems and brain-machine interfaces (BMI) have helped restore functionality and independence to individuals living with sensorimotor deficits; however, tasks requiring bimanual coordination and fine manipulation continue to remain unsolved given the technical complexity of controlling multiple degrees of freedom (DOF) across multiple limbs in a coordinated way through a user input. To address this challenge, we implemented a collaborative shared control strategy to manipulate and coordinate two Modular Prosthetic Limbs (MPL) for performing a bimanual self-feeding task. A human participant with microelectrode arrays in sensorimotor brain regions provided commands to both MPLs to perform the self-feeding task, which included bimanual cutting. Motor commands were decoded from bilateral neural signals to control up to two DOFs on each MPL at a time. The shared control strategy enabled the participant to map his four-DOF control inputs, two per hand, to as many as 12 DOFs for specifying robot end effector position and orientation. Using neurally-driven shared control, the participant successfully and simultaneously controlled movements of both robotic limbs to cut and eat food in a complex bimanual self-feeding task. This demonstration of bimanual robotic system control via a BMI in collaboration with intelligent robot behavior has major implications for restoring complex movement behaviors for those living with sensorimotor deficits.
Modeling the Sequential Pattern Variability of the Electromotor Command System of Pulse Electric Fish
Authors: Lareo Angel , Varona Pablo , Rodriguez Francisco B.
Published: June 2022

Mormyridae, a family of weakly electric fish, use electric pulses for communication and for extracting information from the environment (active electroreception). The electromotor system controls the timing of pulse generation. Ethological studies have described several sequences of pulse intervals (SPIs) related to distinct behaviors (e.g., mating or exploratory behaviors). Accelerations, scallops, rasps, and cessations are four different SPI patterns reported in these fish, each showing characteristic stereotyped temporal structures. This article presents a computational model of the electromotor command circuit that reproduces a whole set of SPI patterns while keeping the same internal network configuration. The topology of the model is based on a simplified representation of the network with four neuron clusters (nuclei). An initial configuration was built to reproduce nucleus characteristics and network topology as described by detailed morphological and electrophysiological studies. Then, a methodology based on a genetic algorithm (GA) was developed and applied to tune the model connectivity parameters to automatically reproduce a whole set of patterns recorded from freely-behaving Gnathonemus petersii specimens. Robustness analyses of input variability were performed to discard overfitting and assess validity. Results show that the set of SPI patterns is consistently reproduced reaching a dynamic balance between synaptic properties in the network. This model can be used as a tool to test novel hypotheses regarding temporal structure in electrogeneration. Beyond the electromotor model itself, the proposed methodology can be adapted to fit models of other biological networks that also exhibit sequential patterns.
Nucleus Accumbens Core Dopamine D2 Receptor-Expressing Neurons Control Reversal Learning but Not Set-Shifting in Behavioral Flexibility in Male Mice
Authors: Macpherson Tom , Kim Ji Yoon , Hikida Takatoshi
Published: June 2022

The ability to use environmental cues to flexibly guide responses is crucial for adaptive behavior and is thought to be controlled within a series of cortico-basal ganglia-thalamo-cortical loops. Previous evidence has indicated that different prefrontal cortical regions control dissociable aspects of behavioral flexibility, with the medial prefrontal cortex (mPFC) necessary for the ability to shift attention to a novel strategy (set-shifting) and the orbitofrontal cortex (OFC) necessary for shifting attention between learned stimulus-outcome associations (reversal learning). The nucleus accumbens (NAc) is a major downstream target of both the mPFC and the OFC; however, its role in controlling reversal learning and set-shifting abilities is still unclear. Here we investigated the contribution of the two major NAc neuronal populations, medium spiny neurons expressing either dopamine D1 or D2 receptors (D1-/D2-MSNs), in guiding reversal learning and set-shifting in an attentional set-shifting task (ASST). Persistent inhibition of neurotransmitter release from NAc D2-MSNs, but not D1-MSNs, resulted in an impaired ability for reversal learning, but not set-shifting in male mice. These findings suggest that NAc D2-MSNs play a critical role in suppressing responding toward specific learned cues that are now associated with unfavorable outcomes (i.e., in reversal stages), but not in the suppression of more general learned strategies (i.e., in set-shifting). This study provides further evidence for the anatomical separation of reversal learning and set-shifting abilities within cortico-basal ganglia-thalamo-cortical loops.
Cellular Tolerance Induced by Chronic Opioids in the Central Nervous System
Authors: Adhikary Sweta , Williams John T.
Published: June 2022

Opioids are powerful analgesics that elicit acute antinociceptive effects through their action the mu opioid receptor (MOR). However opioids are ineffective for chronic pain management, in part because continuous activation of MORs induces adaptive changes at the receptor level and downstream signaling molecules. These adaptations include a decrease in receptor-effector coupling and changes to second messenger systems that can counteract the persistent activation of MORs by opioid agonists. Homeostatic regulation of MORs and downstream signaling cascades are viewed as precursors to developing tolerance. However, despite numerous studies identifying crucial mechanisms that contribute to opioid tolerance, no single regulatory mechanism that governs tolerance in at the cellular and systems level has been identified. Opioid tolerance is a multifaceted process that involves both individual neurons that contain MORs and neuronal circuits that undergo adaptations following continuous MOR activation. The most proximal event is the agonist/receptor interaction leading to acute cellular actions. This review discusses our understanding of mechanisms that mediate cellular tolerance after chronic opioid treatment that, in part, is mediated by agonist/receptor interaction acutely.
Prediction of Soluble Solids and Lycopene Content of Processing Tomato Cultivars by Vis-NIR Spectroscopy
Authors: Égei Márton , Takács Sándor , Palotás Gábor , Palotás Gabriella , Szuvandzsiev Péter , Daood Hussein Gehad , Helyes Lajos , Pék Zoltán
Published: June 2022

Tomato-based products are significant components of vegetable consumption. The processing tomato industry is unquestionably in need of a rapid definition method for measuring soluble solids content (SSC) and lycopene content. The objective was to find the best chemometric method for the estimation of SSC and lycopene content from visible and near-infrared (Vis-NIR) absorbance and reflectance data so that they could be determined without the use of chemicals in the process. A total of 326 Vis-NIR absorbance and reflectance spectra and reference measurements were available to calibrate and validate prediction models. The obtained spectra can be manipulated using different preprocessing methods and multivariate data analysis techniques to develop prediction models for these two main quality attributes of tomato fruits. Eight different method combinations were compared in homogenized and intact fruit samples. For SSC prediction, the results showed that the best root mean squared error of cross-validation (RMSECV) originated from raw absorbance (0.58) data and with multiplicative scatter correction (MSC) (0.59) of intact fruit in Vis-NIR, and first derivatives of reflectance (R2 = 0.41) for homogenate in the short-wave infrared (SWIR) region. The best predictive ability for lycopene content of homogenate in the SWIR range (R2 = 0.47; RMSECV = 17.95 mg kg–1) was slightly lower than that of Vis-NIR (R2 = 0.68; 15.07 mg kg–1). This study reports the suitability of two Vis-NIR spectrometers, absorbance/reflectance spectra, preprocessing methods, and partial least square (PLS) regression to predict SSC and lycopene content of intact tomato fruit and its homogenate.
Exergaming During Ramadan Intermittent Fasting Improve Body Composition as Well as Physiological and Psychological Responses to Physical Exercise in Adolescents With Obesity
Authors: Abedelmalek Salma , Aloui Khouloud , Denguezli Bouzgarou Meriam , Adam Halima , Souissi Nizar , Chtourou Hamdi
Published: June 2022

The effects of exergaming on biochemical responses has been investigated; however, no data is available for this effect during Ramadan intermittent fasting (RIF). RIF is a daily fasting characterized by abstaining from eating and drinking from sunrise to sunset for 29–30 days. The purpose of this study was to investigate the effect of exergaming during RIF on body composition, physical performance and hematological parameters in overweight and adolescents with obesity. Twenty-four adolescents with obesity were divided into two groups [control group (CG), n = 12, or cooperative sport exergaming group (EG), n = 12: 45 min per session during five days per week)]. Participants completed a 6-min walking test (6MWT), a squat jump test and a 10 and a 30m sprint tests in four different occasions: before Ramadan (T0), the second week of Ramadan (T1), the fourth week of Ramadan (T2), and after Ramadan (T3). Blood pressure, rating of perceived exertion (RPE), body composition, central obesity index, dietary intake and profile of mood states (POMS) were, also, assessed over the four periods. The results showed that body weight, body mass index and body fat percentage were significantly lower at T2 compared to T0 and T1 in the EG. After RIF, body composition returned to the values recorded before RIF. The POMS score was significantly lower during T2 compared to T0, T1 and T3 in the EG. The vertical jump and the 6MWT distance were significantly higher (i) at T2 compared to T0, T1 and T3 in the EG and (ii) in EG compared to CG at T2. RPE was significantly lower (i) at T2 compared to T0, T1 and T3 in the EG and (ii) in EG compared to CG at T2. Blood pressure was lower during T2 compared to the other periods in EG. The EG experienced significant decreases in total cholesterol and triglycerides during T2. However, no significant changes between groups and periods was reported for all the other parameters. In conclusion, exergaming during RIF has a positive effect on body composition and physiological and psychological responses in adolescents with obesity.
Sugar Is the Key Cause of Overweight/Obesity in Sugar-Sweetened Beverages (SSB)
Authors: Yu Lianlong , Zhou Han , Zheng Fengjia , Song Jian , Lu Yutong , Yu Xiao , Zhao Changsheng
Published: June 2022

To evaluate association between overweight/obesity and sugar-sweetened beverages (SSB) types, SSB sugar, among children and adolescents. A total of 1,068 children and 751 adolescents were identified from a provincial survey conducted in Shandong, China. χ2 tests, logistic regression, restricted cubic spline (RCS), mediation analysis, pathway analysis, and ordinary least square (OLS) regression were applied to test association among overweight/obesity, SSB sugar, SSB types, and a set of factors. The mean daily SSB intake for children and adolescents was 210.7 and 208 ml, respectively. The threshold of SSB sugar consumption causing overweight/obesity was around 25 g/day and verified by RCS based on logistic regression. Approximately, 44.8% of the study subjects consumed more than 25 g/day of SSB sugar. SSB sugar intake above 25 g/day resulted in higher risk of overweight/obesity (OR = 1.391, 95% CI, 1.115–1.734). The effects of all types of SSBs on overweight/obesity were fully mediated by SSB sugar (p < 0.05), except for milk. Consumption of any types of SSBs had a positive impact on SSB sugar intake in both children and adolescents. Any type of SSB intake was a risk factor in excessive intake of SSB sugar (OR > 1, p < 0.05). In particular, milk powder, tea, and tea-flavored drinks, and carbonated have greater ORs for excessive intake of SSB sugar (OR = 76.08, 8.879, 4.355, p < 0.05, respectively). It was found that the effect of SSB on overweight/obesity was mediated by the intake of SSB sugar, and the effects of various SSBs were different according to multiple linear regression and pathway analysis (p < 0.05, respectively).
Free and Bound Phenolic Profiles of Rosa roxburghii Tratt Leaves and Their Antioxidant and Inhibitory Effects on α-Glucosidase
Authors: Yang Yuzhe , Li Wu , Xian Wenyan , Huang Wei , Yang Ruili
Published: June 2022

Rosa roxburghii Tratt (R. roxburghii) tea is a traditional Chinese beverage. This study aims to investigate and compare the phenolics in free and bound forms of two cultivars of R. roxburghii leaves, and their bioactivities. The total phenolic content of free and bound fractions was 72.71 and 17.75 mg GAE/g DW in Gui Nong No. 5 (GNN5) and 94.28 and 11.19 mg GAE/g DW in Seedless Cili (SC). A total of 37 phenolic compounds were characterized and quantified by UPLC-Q-Exactive Orbitrap/MS with ellagic acid, quercitrin, isoquercitrin, and quininic acid in free fraction, while gallic acid, ellagic acid, and hyperoside were main compounds in bound fraction. The free fraction with higher phenolic contents also showed excellent performances on antioxidant activities and α-glucosidase inhibitory potency than bound phenolics. Therefore, the results highlight that R. roxburghii leaves are a promising source enriched in phenolic constituents for functional beverages and nutritional foods.
Identification and Validation of Prognostic Related Hallmark ATP-Binding Cassette Transporters Associated With Immune Cell Infiltration Patterns in Thyroid Carcinoma
Authors: Wang Lidong , Sun Xiaodan , He Jingni , Liu Zhen
Published: June 2022

ATP-binding cassette (ABC) transporters are a large superfamily of membrane proteins that facilitate the translocation of heterogeneous substrates. Studies indicate that ABC transporters may play important roles in various carcinomas. However, the correlation between ABC transporters and immunomodulation in thyroid carcinoma (TC), as well as the prognoses for this disease, is poorly understood.TC data from The Cancer Genome Atlas (TCGA) database were used to identify prognostic hallmark ABC transporters associated with immune cell infiltration patterns via multiple bioinformatic analyses. Thereafter, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression of these selected hallmark ABC transporters in both TC and para-cancerous thyroid tissues. Of a total of 49 ABC transporters, five (ABCA8, ABCA12, ABCB6, ABCB8, and ABCC10) were identified as hallmark ABC transporters. All five were differentially expressed in TC and associated with the relapse-free survival rates of patients with TC. Immunoregulation by these five hallmark ABC transporters involved the modulation of various aspects of immune cell infiltration, such as hot or cold tumor subsets and the abundances of infiltrating immune cells, as well as specific immunomodulators and chemokines. Besides the diverse significantly correlated factors, the five hallmark ABC transporters and correlated genes were most highly enriched in plasma membrane, transporter activity, and transmembrane transport of small molecules. In addition, many chemicals, namely bisphenol A and vincristine, affected the expression of these five transporters. The qRT-PCR results of collected TC and para-cancerous thyroid tissues were consistent with those of TCGA. The findings in this study may reveal the role played by these five hallmark ABC transporters in regulating immune cell infiltration patterns in TC as well as the molecular mechanisms underlying their functions, leading to a better understanding of their potential prognostic and immunotherapeutic values.
Skeletal Muscle Mass Measurement Using Cone-Beam Computed Tomography in Patients With Head and Neck Cancer
Authors: Huang Wei , Tan Peixin , Zhang Hongdan , Li Zhen , Lin Hui , Wu Youxing , Du Qinwen , Wu Qidi , Cheng Jun , Liang Yu , Pan Yi
Published: June 2022

Background and purpose

Head and neck cancer (HNC) patients usually present with malnutrition during radiotherapy, leading to loss of skeletal muscle mass (SMM) and poor clinical outcomes. CT has been used in clinical practice for measuring SMM in cancer patients. However, its clinical application for monitoring SMM is limited by the expensive price and high radiation exposure. This study aimed to investigate the feasibility of cone-beam computed tomography (CBCT) for assessing SMM and its changes in HNC patients undergoing radiotherapy.

Materials and methods

This study was divided into two parts. In part 1 (n = 32), the cross-sectional of skeletal muscle area (SMA) at the third cervical vertebra (C3) based on CBCT and computed tomography (CT) was assessed. In part 2 (n = 30), CT and CBCT were performed, and patients’ weight was measured before and at four different time points during radiotherapy. SMAs at C3 were independently identified by three senior radiation oncologists. The interobserver agreement of SMA on CBCT (SMACBCT) findings was analyzed using the intraclass correlation coefficient (ICC). One-way analysis of variance was used to evaluate the interobserver variability and statistical significance for SMA measurements. CBCT and CT measurement differences and correlations were analyzed using paired sample t-test and Pearson correlation analysis, respectively. The Krouwer variant of the Bland–Altman plot was used to analyze the agreement of SMA measurements between CBCT and CT. A simple linear regression model was used to analyze the relationship of SMA measurements between the two imaging techniques, and the equation was established. A repeated-measures ANOVA was performed to evaluate the effects and interactions between weight loss, SMA loss, and time.

Results

SMACBCT demonstrated excellent interobserver reliability; no significant difference between SMACBCT and SMA on CT (SMACT) at C3 was observed in all patients. The SMACBCT and SMACT were highly correlated (r = 0.966; 95% confidence interval = 0.955–0.975; p < 0.001). Bland–Altman analysis revealed that SMACBCT was generally higher than SMACT. The predicted SMA value at C3 on CT using CBCT was similar to the actual value. Moreover, significant differences between SMA and weight loss (F =10.99, p = 0.002), groups (weight loss and SMA loss) and times (4 time points) (F = 3.93, p = 0.013), and mean percent loss over time (F = 7.618, p < 0.001) were noted.

Conclusion

CBCT may be used as an alternative for CT to measure SMA in HNC patients during radiotherapy.
A Genetic Variant in CD274 Is Associated With Prognosis in Metastatic Colorectal Cancer Patients Treated With Bevacizumab-Based Chemotherapy
Authors: Qin Wan , Zhao Ben , Wang Duanrui , Liu Jiamin , Zhou Yilu , Zhu Wenjun , Huang Yongbiao , Qiu Hong , Yuan Xianglin
Published: June 2022

Bevacizumab plus chemotherapy is a well-established first-line treatment for metastatic colorectal cancer (mCRC). We investigated whether polymorphisms of genes involved in immune regulation signaling are related to the clinical outcome of mCRC patients treated with bevacizumab-based chemotherapy. In this study, we genotyped 14 single-nucleotide polymorphisms (SNP) in IFN-γ/IFNGRs/JAKs/STATs/PD-L1 pathway by using DNA from blood samples of 141 mCRC patients treated with first-line bevacizumab-based chemotherapy. In the univariate and multivariate analysis, patients with AA genotype of CD274:rs2297136 had a significantly better PFS and OS than patients with AG or GG genotype (10.8 versus 9.8, log-rank P=0.0031; 31.4 versus 20.9, log-rank P=0.0233). Patients with AG/GG genotype of IFNGR1:rs2234711, CT/TT genotype of IFNGR1:rs9376267 also showed longer OS than patients with AA or CC genotype, however, the statistic did not reach significant after adjusted by clinical factors in the multivariate analysis. A nomogram based on the genetic variants and clinic characteristics was developed with a good accuracy to predict patients’ survival. Our result indicates that CD274:rs2297136 is one of the most important predictors for the prognosis of mCRC patients treated with bevacizumab-based chemotherapy, if further validated in larger population.
Nanoparticle-Based Combination Therapy for Melanoma
Authors: Chen Hongbo , Hou Kai , Yu Jing , Wang Le , Chen Xue
Published: June 2022

Melanoma is a cutaneous carcinoma, and its incidence is rapidly increasing with every year. The treatment options for melanoma have been comprehensively studied. Conventional treatment methods (e.g., radiotherapy, chemotherapy and photodynamic therapy) with surgical removal inevitably cause serious complications; moreover, resistance is common. Nanoparticles (NPs) combined with conventional methods are new and promising options to treat melanoma, and many combinations have been achieving good success. Due to their physical and biological features, NPs can help target intended melanoma cells more efficiently with less damage. This creates new hope for a better treatment strategy for melanoma with minimum damage and maximum efficacy.
Biocompatible Supramolecular Mesoporous Silica Nanoparticles as the Next-Generation Drug Delivery System
Authors: Mohamed Farahidah , Oo May K. , Chatterjee Bappaditya , Alallam Batoul
Published: June 2022

Supramolecular mesoporous silica nanoparticles (MSNs) offer distinct properties as opposed to micron-sized silica particles in terms of their crystal structure, morphology–porosity, toxicity, biological effects, and others. MSN biocompatibility has touched the pharmaceutical realm to exploit its robust synthesis pathway for delivery of various therapeutic molecules including macromolecules and small-molecule drugs. This article provides a brief review of MSN history followed by special emphasis on the influencing factors affecting morphology–porosity characteristics. Its applications as the next-generation drug delivery system (NGDDS) particularly in a controlled release dosage form via an oral drug delivery system are also presented and shall be highlighted as oral delivery is the most convenient route of drug administration with the economical cost of development through to scale-up for clinical trials and market launch.
Astragalus mongholicus Bunge (Fabaceae): Bioactive Compounds and Potential Therapeutic Mechanisms Against Alzheimer’s Disease
Authors: Dong Qianyu , Li Zhen , Zhang Qian , Hu Yueyu , Liang Huazheng , Xiong Lize
Published: June 2022

Astragalus mongholicus Bunge (Fabaceae) (also known as Astragali radix-AR), a widely used herb by Traditional Chinese Medicine practitioners, possesses a wide range of pharmacological effects, and has been used to treat Alzheimer’s disease (AD) historically. Its bioactive compounds are categorized into four families: saponins, flavonoids, polysaccharides, and others. AR’s bioactive compounds are effective in managing AD through a variety of mechanisms, including inhibiting Aβ production, aggregation and tau hyperphosphorylation, protecting neurons against oxidative stress, neuroinflammation and apoptosis, promoting neural stem cell proliferation and differentiation and ameliorating mitochondrial dysfunction. This review aims to shed light upon the chemical constituents of AR and the mechanisms underlying the therapeutic effect of each compound in manging AD. Also presented are clinical studies which reported successful management of AD with AR and other herbs. These will be helpful for drug development and clinical application of AR to treat AD.
Network Robustness Revisited
Authors: Gross Thilo , Barth Laura
Published: June 2022

The robustness of complex networks was one of the first phenomena studied after the inception of network science. However, many contemporary presentations of this theory do not go beyond the original papers. Here we revisit this topic with the aim of providing a deep but didactic introduction. We pay attention to some complications in the computation of giant component sizes that are commonly ignored. Following an intuitive procedure, we derive simple formulas that capture the effect of common attack scenarios on arbitrary (configuration model) networks. We hope that this easy introduction will help new researchers discover this beautiful area of network science.
Feasibility Analysis of Sapphire Compound Refractive Lenses for Advanced X-Ray Light Sources
Authors: Wang Yunzhu , Dong Xiaohao , Hu Jun
Published: June 2022

The compound refractive lens (CRL) is a commonly used X-ray optical component for photon beam conditioning and focusing on the beamlines of the X-ray facilities. The normal preparation materials are beryllium, aluminum, silicon of current lenses, and they all suffered from high heat load fatigue and short pulse damage risks. Hard materials based CRL is engaged attention for the advanced X-ray application. Sapphire crystal has the advantages of high density, high melting point, low thermal expansion coefficient. In this paper, properties of the refraction and absorption ratio of Sapphire and parameters of Sapphire lenses of effective aperture, transmittance, resolution, number of lenses needed for a certain focus, are taken into account for the CRL design, comparing with those of several common materials as well. The calculation results show that the performance of the sapphire lens is better than that of the aluminum lens and silicon lens, and inferior to that of the beryllium lens and diamond lens, but the number of lenses used is less. In the meantime, performances of sapphire lenses focusing are simulated and thermal effects on lenses are analyzed. Analysis and discussion are carried out under the same conditions as the metal Aluminum ones. The focusing simulation shows that the sapphire lenses can obtain a smaller spot with more intensity. The thermal analysis indicates that the temperature during use of the sapphire lens is much lower than the melting point of sapphire, and the thermal deformation is negligible.
A High-Power Radar Rotary Joint
Authors: Zhang Zhiqiang , Xue Kaibin , Weng Zibin
Published: June 2022

Due to the power capacity limitation of the rotary joint, mechanical scanning radars are limited to use high-power microwave sources to improve their performance furthermore. To solve this problem, an over-mode circular waveguide rotary joint with radial mutations at the rotation point is introduced in this letter. The structure of the radial mutation is optimized to suppress unwanted modes. By connecting the choke slot and the rotating part, the breakdown risks of the rotary joint can be reduced. In addition, the choke structure is connected to the inner wall of the waveguide with a gap, even the breakdown occurs in the choke, the normal modes inside the waveguide will not be affected. To verify the design, a prototype of the rotary joint is fabricated and measured with the operating band in the range of 9.5–10.5 GHz and a power capacity of 3 GW.
Effect of Acute Cold Exposure on Energy Metabolism and Activity of Brown Adipose Tissue in Humans: A Systematic Review and Meta-Analysis
Authors: Huo Chuanyi , Song Zikai , Yin Jianli , Zhu Ying , Miao Xiaohan , Qian Honghao , Wang Jia , Ye Lin , Zhou Liting
Published: June 2022

Background: The benefit of cold exposure for humans against obesity has brought the energy metabolism and activity of brown adipose tissue (BAT) induced by cold into focus. But the results are inconsistent. This review is aimed to systematically explore the effect of cold exposure on the activity of BAT and energy metabolism in humans.

Methods: We searched relevant papers that were published from 1990 to 2021 and were cited in PubMed Central, Web of science, Embase and Cochrane Library databases to conduct this systematic review and meta-analysis. Energy metabolism, BAT volume, BAT activity and non-esterified fatty acids (NEFA) data reported in eligible researches were extracted. Meta-analysis was applied to combine the mean difference or standard mean difference with their 95% confidence intervals (95%CI). RevMan 5.3 software was used for meta-analysis and evaluating the risk of bias. Stata 16.0 was used for evaluating the publication bias.

Results: Ten randomized controlled trials were included in meta-analysis. Compared with human exposed in room temperature at 24°C, the energy expenditure (EE) was increased after acute cold exposure at 16∼19°C (Z = 7.58, p < 0.05, mean different = 188.43kal/d, 95% CI = 139.73–237.13); BAT volume (Z = 2.62, p < 0.05; standard mean different = 0.41, 95% CI = 0.10–0.73); BAT activity (Z = 2.05, p = 0.04, standard mean difference = 1.61, 95% CI = 0.07–3.14) and the intake of BAT NEFA (Z = 2.85, p < 0.05; standard mean different = 0.53, 95% CI = 0.17–0.90) also increased.

Conclusion: Acute cold exposure could improve the energy expenditure and BAT activity in adults, which is beneficial for human against obesity.
Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067”
Authors: Zhang Peipei , Lan Caixia , Singh Ravi P. , Huerta-Espino Julio , Li Zaifeng , Lagudah Evans , Bhavani Sridhar
Published: June 2022

Leaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have been a valuable resource for mining new genes for various abiotic and biotic stresses including wheat rusts. Afghan wheat landrace “KU3067” displayed high seedling infection type (IT) for leaf rust and low IT for stripe rust; however, it displayed high levels of field resistance for both rusts when tested for multiple seasons against the Mexican rust isolates. This study focused on identifying loci-conferring seedling resistance to stripe rust, and also loci-conferring adult plant resistance (APR) against the Mexican races of leaf rust and stripe rust. A backcrossed inbred line (BIL) population advanced to the BC1F5 generation derived from the cross of KU3067 and Apav (triple rust susceptible line) was used for both, inheritance and QTL mapping studies. The population and parents were genotyped with Diversity Arrays Technology-genotyping-by-sequencing (DArT-Seq) and phenotyped for leaf rust and stripe rust response at both seedling and adult plant stages during multiple seasons in Mexico with relevant pathotypes. Mapping results identified an all-stage resistance gene for stripe rust, temporarily designated as YrKU, on chromosome 7BL. In total, six QTL-conferring APR to leaf rust on 1AS, 2AL, 4DL, 6BL, 7AL, and 7BL, and four QTL for stripe rust resistance on 1BS, 2AL, 4DL, and 7BL were detected in the analyses. Among these, pleiotropic gene Lr67/Yr46 on 4DL with a significantly large effect is the first report in an Afghan landrace-conferring resistance to both leaf and stripe rusts. QLr.cim-7BL/YrKU showed pleiotropic resistance to both rusts and explained 7.5–17.2 and 12.6–19.3% of the phenotypic variance for leaf and stripe rusts, respectively. QYr.cim-1BS and QYr.cim-2AL detected in all stripe environments with phenotypic variance explained (PVE) 12.9–20.5 and 5.4–12.5%, and QLr.cim-6BL are likely to be new. These QTL and their closely linked markers will be useful for fine mapping and marker-assisted selection (MAS) in breeding for durable resistance to multiple rust diseases.
Transcriptional Proposition for Uniquely Developed Protocorm Flowering in Three Orchid Species: Resources for Innovative Breeding
Authors: Ahmad Sagheer , Chen Jinliao , Chen Guizhen , Huang Jie , Hao Yang , Shi Xiaoling , Liu Yuying , Tu Song , Zhou Yuzhen , Zhao Kai , Lan Siren , Liu Zhongjian , Peng Donghui
Published: June 2022

During orchid seed culture, seeds germinate as protocorms, and protocorms normally develop into plant with leaves and roots. Orchids require many years of vegetative development for flowering. However, under a certain combination of growth cultures, we observed that protocorms can directly flower without leaves and roots. Therefore, we performed comparative transcriptome analysis to identify the different transcriptional regulators of two types of protocorms of Cymbidium ensifolium, Cymbidium sinense, and Cymbidium goeringii. Zinc finger, MYB, AP2, and bHLH were the most abundant transcription factor (TF) families in the transcriptome. Weighted gene coexpression network analysis (WGCNA) was performed to identify hub genes related to leaf and flower development. The key hubs included SPL6, SVP, SEP2, KNOX1, AP2, OFP1, COL12, MYB13, MYB36, MYB59, bHLH086, and ARF7. The hub genes were further validated through statistical tools to propose the roles of key TFs. Therefore, this study initiates to answer that why there is no leaf initiation and root development and how can protocorm bypass the vegetative phase to flower? The outcomes can direct future research on short-span flowering in orchids through protocorms.