bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Genetic Landscape of Sepsis-Induced Cardiomyopathy: A Comprehensive Analysis
Authors: Weng Jinsen , Wang Xiaojing , Shang Xiuling , Xiao Jun , Ye Yong , Yu Rongguo , Shang Junya
Published: January 2024

Background: Sepsis poses a severe threat in critical care, often leading to septic cardiomyopathy. This study aimed to explore the genes related to mitochondrial damage in septic cardiomyopathy, observe their changes during sepsis, and analyze the possible pathogenesis of this disease.
Methods: Bioinformatics methods were used to identify differentially expressed genes (DEGs) and enrichment pathways associated with mitochondrial damage in patients with septic cardiomyopathy. Subsequently, clinical specimens and cardiomyocytes were verified and compared to clarify the expression of these genes and their change trend in the pathogenesis of septic myocardial injury to explore the possible relationship between these genes and septic myocardial mitochondrial dysfunction.
Results: In this study, using diverse datasets, 398 differentially expressed genes (DEGs) related to sepsis were identified, and 11 key genes (GNAS, MRPL2, TIMM17b, SLC25A3, SDHA, PRPF6, LMF2, IMMT, CS, UCP2, and CASP2) were significantly associated with these genes. Functional analysis highlighted the importance of the TIM23 complex in septic mitochondrial injury. Real-time fluorescence quantitative PCR was performed on 11 genes and TIMM23 expression in 24-48 hours in clinical specimens, and the expression of TIMM17b and TIMM23 was increased in the sepsis group, while the expression of the other 10 DEGs was decreased. Further verification via cell experiments revealed that the expression of 11 DEGs and 5 TIM23 complex member genes, TIMM23, TIMM17A, TIMM44, PAM16 and TIMM50, increased in the 6-hour group, while their expression decreased significantly in the 24-hour group; moreover, the expression of only TIMM17b was still greater than that in the normal control group. The expression of other genes was lower than or close to that of the normal control group.
Conclusion: This integrative study not only provides a comprehensive overview of DEGs associated with sepsis but also emphasizes the importance of the TIM23 complex. The identified genes and pathways offer potential targets for further mechanistic studies and therapeutic interventions in the context of sepsis-related complications.
Nomogram prediction model for the risk of intracranial hemorrhagic transformation after intravenous thrombolysis in patients with acute ischemic stroke
Authors: Ma Yong , Xu Dong-Yan , Liu Qian , Chen He-Cheng , Chai Er-Qing
Published: January 2024

Background: Hemorrhagic transformation (HT) after intravenous thrombolysis (IVT) leads to poor clinical prognosis, and a reliable predictive system is needed to identify the risk of hemorrhagic transformation after IVT.
Methods: Retrospective collection of patients with acute cerebral infarction treated with intravenous thrombolysis in our hospital from 2018 to 2022. 197 patients were included in the research study. Multivariate logistic regression analysis was used to screen the factors in the predictive nomogram. The performance of nomogram was assessed on the basis of area under the curve (AUC-ROC) of subjects' work characteristics, calibration plots and decision curve analysis (DCA).
Results: A total of 197 patients were recruited, of whom 24 (12.1%) developed HT. In multivariate logistic regression model National Institute of Health Stroke Scale (NIHSS) (OR, 1.362; 95% CI, 1.161 −1.652; P = 0.001), N-terminal pro-brain natriuretic peptide (NT-pro BNP) (OR, 1.012; 95% CI, 1.004 −1.020; P = 0.003), neutrophil to lymphocyte ratio (NLR) (OR, 3.430; 95% CI, 2.082 −6.262; P < 0.001), systolic blood pressure (SBP) (OR, 1.039; 95% CI, 1.009 −1.075; P = 0.016) were the independent predictors of HT which were used to generate nomogram. The nomogram showed good discrimination due to AUC-ROC values. Calibration plot showed good calibration. DCA showed that nomogram is clinically useful.
Conclusion: Nomograms consisting of NIHSS, NT-pro BNP, NLR, SBP scores predict the risk of HT in AIS patients treated with IVT.
Puerarin-containing Rhein-crosslinked Hydrogel for Antibacterial and Anti-Inflammatory Wound Dressings
Authors: Zheng Yu , Li Zhibei , Xu Min , Lu Yongping , Li Xiaofang
Published: January 2024

Wound infections put patients at risk for serious physical harm, even death, and place a heavy financial strain on society. As such, they are one of the most pressing issues facing society today. There is an immediate need for hydrogel dressings with immune-regulating and antibacterial properties. This work is predicated on the two small natural molecules’ complementary physical and efficacious properties. An injectable hydrogel is created by loading the puerarin (PUE) into tyramine-modified hyaluronic acid, then adding rhein (RHE) and H2O2/horseradish peroxidase to facilitate the formation of inter-phenol crosslinks (HA-Tyr-R@P). With its ability to release oxygen and scavenge active oxygen, the developed hydrogel exhibits excellent biocompatibility and modifies the microenvironment favorably. HA-TYR-R@P hydrogels have an antibacterial rate of over 95% and a wound closure rate twice as high as the control group. HA-TYR-R@P hydrogels can inhibit bacteria, reduce inflammation in wounds, encourage wound healing, and have good biocompatibility because of their matched physicochemical characteristics and synergistic therapeutic effects. This work emphasizes the creation of a single-component, multipurpose hydrogel platform that eliminates the need for complex preparations and side effects associated with drugs. Additionally, it provides a comprehensive approach for creating RHE-based hydrogel for wound healing and highlights the importance of natural small molecules in the advancement of biological materials.
MiR-574-5p activates human TLR8 to promote autoimmune signaling and lupus
Authors: Wang Tao , Song Dan , Li Xuejuan , Luo Yu , Yang Dianqiang , Liu Xiaoyan , Kong Xiaodan , Xing Yida , Bi Shulin , Zhang Yan , Hu Tao , Zhang Yunyun , Dai Shuang , Shao Zhiqiang , Chen Dahan , Hou Jinpao , Ballestar Esteban , Cai Jianchun , Zheng Feng , Yang James Y.
Published: January 2024

Endosomal single-stranded RNA-sensing Toll-like receptor-7/8 (TLR7/8) plays a pivotal role in inflammation and immune responses and autoimmune diseases. However, the mechanisms underlying the initiation of the TLR7/8-mediated autoimmune signaling remain to be fully elucidated. Here, we demonstrate that miR-574-5p is aberrantly upregulated in tissues of lupus prone mice and in the plasma of lupus patients, with its expression levels correlating with the disease activity. miR-574-5p binds to and activates human hTLR8 or its murine ortholog mTlr7 to elicit a series of MyD88-dependent immune and inflammatory responses. These responses include the overproduction of cytokines and interferons, the activation of STAT1 signaling and B lymphocytes, and the production of autoantigens. In a transgenic mouse model, the induction of miR-574-5p overexpression is associated with increased secretion of antinuclear and anti-dsDNA antibodies, increased IgG and C3 deposit in the kidney, elevated expression of inflammatory genes in the spleen. In lupus-prone mice, lentivirus-mediated silencing of miR-574-5p significantly ameliorates major symptoms associated with lupus and lupus nephritis. Collectively, these results suggest that the miR-574-5p-hTLR8/mTlr7 signaling is an important axis of immune and inflammatory responses, contributing significantly to the development of lupus and lupus nephritis.
The Main Features of Food Consumption Mobile Apps in Saudi Arabia: A Scoping Review
Authors: Alammari Duaa , Alruhimi Mayasem , Alkhunein Sarah , Alabdulkarim Aljawharah
Published: January 2024

Background
Food consumption apps seem to be supportive digital tools for facilitating weight management and facilitating healthier eating habits. There is a wide variety of food consumption apps available; however, there is limited evidence that determines the main features of these apps. Therefore, this study aimed to identify the scope of prominent food consumption mobile apps across two commercial app stores (Apple iOS and Google Play) and to determine the main features of the apps systematically.
Methods
This scoping review was conducted across the Apple iOS and Google Play Stories in three steps: (1) an electronic app search, (2) the extraction of data, and (3) the analysis of app characteristics and features.
Results
A total of 99 apps were included, 41 (41%) of which were from the iOS store and 58 (59%) of which were from the Google Play store. Approximately 52% of the apps were rated “4.5 or less”, and 48% of the apps were rated “4.6 and above”. Most of the apps offered more than one language, 81% of which provided 1 to 6 languages, and only 10.1% of the apps offered 7 to 12 languages. We identified a total of 23 features; 29% of the apps had 13 to 18 features, 27% of the apps had 7 to 12 features, and 23% of the apps had 19 + features. In contrast, 18.2% of the apps had 1 to 6 features.
Conclusions
The findings of this review indicate that while a wide range of food consumption apps are available, most apps offer limited features. Therefore, there is a need to introduce unique features, such as entering food by picture or barcode scanning, food composition tables, and tracking of micronutrients, to attract more users. In addition, the number of features was shown to be associated with app ratings. Notably, high ratings may increase the app's visibility; therefore, this factor should be considered by app developers when adding desirable features in the future.
Structure simplification of GNE-140 and discovery of a pyridone based analogue as a potential lead for hLDHA inhibitors
Authors: Mo Wen-Hui , Luo Zhi-Yong , Liu Su-You , Ma Da-You
Published: January 2024

In this paper, taking GNE-140, a structurally complicated hLDHA inhibitor, as a lead compound, 19 simple analogues with a pyrimidone, pyranone or pyridone core were designed and conveniently synthesized. Subsequent biological evaluation indicated that although the pyrimidone core had a weak contribution to hLDHA inhibition, compounds with pyranone or pyridone core exhibited better anti-hLDHA activity. Notably, pyridone-bearing compound 1c showed significant selective inhibition of hLDHA (IC50 = 19.5 ± 2.9 µM) over hLDHB (IC50 = 117.6 ± 13.2 µM). Meanwhile, 1c showed inhibition on the lactate production of lung cancer cell A549. Thus, the result of this study indicated 1c is worthy of further study owing to its convenient synthesis and strong selective hLDHA inhibition.
Oncologists’ perspective on Advance Directives, a French national prospective cross-sectional survey – the ADORE Study
Authors: Cambriel Amélie , Serey Kevin , Pollina-Bachellerie Adrien , Cancel Mathilde , Michalet Morgan , Bay Jacques-Olivier , Bouleuc Carole , Lotz Jean-Pierre , Philippart Francois
Published: January 2024

Background
The foreseeable severity of the prognosis of cancer invites to offer patients the possibility to express their values for care management. However, the prevalence of advance directives(AD) among oncology patients remains low. The aim of this study was to measure the interests and limits to the implementation of AD from the perspective of oncologists.
Methods
French national online survey of hospital-based oncologists. The questionnaire was composed of 5 domains: information on AD, redaction assistance, usage of AD, personal perception of the interest of ADs and clinical particularities of the respondent. Primary outcome was the reported frequency of patients’ information about existence of AD. Secondary outcomes were the measure of factors associated and barriers to the provision of AD information.
Results
410 oncologists (50%) responded to the survey. 75% (n = 308) considered AD as a relevant tool. 36% (n = 149) regularly inform their patients. 25% (n = 102) remain suspicious of this provision, either because they see it as a source of anxiety (n = 211; 60%) or that patients require specific information to write relevant AD (n = 367; 90%). Experience in palliative care was associated with more frequent approach of AD (43% vs 32.3%, p = 0.027). Previous experience of critical care was associated with higher levels of AD distrust (31.5% vs 18.8%, p = 0.003). 68.5% (n = 281) of respondents considered a person of trust more appropriate than AD.
Conclusion
Although AD are mostly considered as a relevant tool, only one third of oncologists regularly inform their patients about them. Uncertainty about the safety and relevance of AD remain high.
Death, Inequality, and the Pandemic in the Nation’s Capital
Authors: Alva Maria L. , Illa Srujana S. , Haber Jaren
Published: January 2024

Background.
Abrupt changes in mortality rates and life expectancy allow us to understand how shocks like COVID-19 can exacerbate health inequalities across groups. We look at Washington, D.C., a major city with a diverse population and long-standing socio-economic divisions. Because DC is a socioeconomically and racially diverse city, the data describing all-cause mortality, cause-specific mortality, and life expectancy provides an informative case study to understand how the pandemic has widened pre-existing gaps across socio-demographic groups.
Methods.
Death certificates for 2015–2021 from the DC Department of Health’s Vital Records and the Census Bureau’s American Community Survey annual estimates are used to compute mortality rate trends by age, sex, race, and residence location. Using the life table approach, we calculate years of life lost (YLL) attributable to COVID-19.
Results.
We find that in 2020 and 2021, there were respectively 1,128 and 629 excess deaths (158 per 100K and 94 per 100K) compared to the annual average over the previous five years, and 689 and 363 deaths in 2020 and 2021, respectively (97 per 100K and 54 per 100K) listing COVID-19 as a cause of death. Accidental and violent deaths increased disproportionately by race. Death rates in 2020 and 2021, compared to the five previous years, were higher for men than women by about 12pp and 5pp and occurred almost entirely among residents 45 and older. Excess deaths between 2020 and 2021 were higher for Black and Hispanic residents by about 286 and 97 per 100K, respectively—with the highest proportional increase (almost twofold) for Hispanics in 2020. Life expectancy in the United States decreased for the first time in 2020 by 1.5 years. In the nation’s capital, the decline in life expectancy was 2.7 years but YLL was highest for Hispanic males and lowest for White females.
Conclusions.
Local-level sociodemographic research like ours reveals intersectional inequalities often obscured by national statistics used in popular and scholarly discourse. The demographic disparities in life expectancy exacerbate existing longevity gaps, which could have severe intergenerational consequences above and beyond the pandemic's immediate morbidity and mortality impacts.
Fluorescent Carbon Dots: Aggregation-Induced Emission Enhancement, Application as probe for CN - and Cr 2 O 7 -2 , sensing strips and bio-imaging agent
Authors: Bhatt Madhuri , Bhatt Shreya , Vyas Gaurav , Raval Ishan , Kumar Anshu , Paul Parimal
Published: January 2024

Fluorescent carbon dots (Trp-CDs) were prepared using tryptophan as precursor and were characterized on the basis of elemental analysis, powder-XRD, IR, Raman spectroscopy, 13C-NMR, UV-Vis, fluorescence and TEM. Trp-CDs exhibit poor fluorescence in 100% water but showed strong Aggregation Induced Emission (AIE) in ethanol and higher alcohols. The anion sensing study of Trp-CD revealed that it selectively detects CN− and Cr2O7 − 2 and from fluorescence quenching titration study, quenching constant, LOD and range of detection were evaluated. The emission life-time of Trp-CD before and after addition of CN− and Cr2O7 − 2 were measured, the decay curve before addition of anion was best fitted with a bi-exponential function with life-time of τ1 2.79 ns (10.74%) and τ2 18.93 ns (89.26%). The mechanistic study revealed that for CN−, the fluorescence quenching is due to its interaction with protons attached to surface functional groups and for Cr2O7 − 2, it is due to inner filter effect (IFE). Sensing strips were prepared by coating Trp-CDs onto various solid surfaces including agarose films and were used for detection of CN− and Cr2O7−. Trp-CD was found to be nontoxic and biocompatible and used as staining agent for Artemia and Bacteria (Bacillus Subtilis, Pseudomonas) and detection of CN− and Cr2O7−.
Chronic pelvic pain in congestion pelvic syndrome: clinical impact and electromyography pelvic floor activity prior and after endovascular treatment
Authors: Corvino Fabio , Giurazza Francesco , Coppola Milena , Tomasello Antonio , Coletta Francesco , Sala Crescenzo , de Martino Bernardo Maria , corvino Antonio , Niola Raffaella
Published: January 2024

Background
To characterize clinical impact of endovascular treatment in Chronic Pelvic Pain (CPP) patients due to Pelvic Congestion Syndrome (PCS) and to assess the diagnostic value of surface electromyography (sEMG) study of pelvic floor musculature (PFM) in PCS patients pre- and post-endovascular treatment. Between January 2019 and July 2023, we studied consecutive patients who were referred for interventional radiology assessment and treatment to a tertiary trauma care Hospital, had evidence of non-obstructive PCS by Magnetic Resonance Imaging (MRI), had performed a sEMG of PFM and underwent endovascular treatment. The primary outcome was clinical, defined as change in symptoms severity after endovascular treatment. The secondary outcome was a difference in the sEMG values pre- and after endovascular therapy.
Results
We included 32 women (mean age 38 years). CPP was the leading symptom in 100% patients, followed by dysmenorrhea (75%) and post-coital pain (68.7%). Endovascular therapy included ovarian vein embolization in 28 patients (87.5%) and internal iliac vein embolization in only 2 patients (6.2%). After a median of 8 (range 6–10) months from endovascular treatment, 29 (90%) of patients reported improvement of the main symptoms, and 15 (46%) were symptom-free. The sEMG values didn’t show a statistical difference pre- and after PCS endovascular treatment.
Conclusions
Endovascular treatment appeared to be highly effective in CPP due to PCS and was associated with a low rate of complication. sEMG study could be useful in revealing alteration of PFM electrophysiology, but a difference pre and after embolization in PCS patient wasn’t demonstrated.
Long-term Outcomes of Surgical Intervention for Symptomatic Aural Atresia and Stenosis
Authors: Yang Runqin , Zhao Yu , Du Miaomiao , Li Rui , Dong Chen , Zha Dingjun , Han Yu
Published: January 2024

Background
This study aims to analyze the clinic features of symptomatic aural atresia and stenosis, and to explore the surgical techniques and postoperative care for aural atresia and stenosis.
Methods
A retrospective analysis on 24 patients with aural atresia (nine patients) or stenosis (15 patients) was conducted. They had done surgery and followed over one years. The basic information, etiologies, symptoms, with or without cholesteatoma were collected. For preventing postoperative restenosis, a plastic sheet with expansive sponge was commonly used as support material. And triamcinolone acetonide was locally injected when a stenotic tendency occurred. Additionally, three representative cases are presented.
Results
The main symptoms were hearing loss, otorrhea and otalgia. Cholesteatoma appeared in 10 patients (66.7%, 10/15) with aural stenosis and six patients (66.7%, 6/9) with aural atresia. After surgery intervention, the air-bone gaps (ABG) in both stenosis and atresia group significantly improved. Restenosis was observed in two patients (8.3%, 2/24) with aural stenosis. The etiologies of them were congenital and acquired, respectively. Tympanic membrane (TM) lateralization occurred in one patient (4.2%, 1/24) with aural atresia. Intralesional injection of triamcinolone acetonide was performed in two patients. One received injection three times and obtained a satisfying outcome. However, the other one failed to continue this treatment and restenosis occurred.
Conclusion
Surgical intervention is a gold standard for symptomatic aural stenosis and aural atresia. To achieve a promising outcome, a plastic sheet with expansive sponge was helpful to support the new EAC and a regular follow-up was mandatory. When a restenosis tendency occurred, intralesional injection of triamcinolone acetonide might benefit to improve it.
Prenatal Utility of Chromosomal Microarray Analysis and Pregnancy Outcomes ： A Cohort Study of 4211 Pregnancies
Authors: Li Huafeng , Hu Juan , Wu Qingyu , Qiu Jigang , Zhang Li , Zhu Jinping
Published: January 2024

With the gradual liberalization of the three-child policy and the development of assisted reproductive technology in China, the population of high-risk pregnancies is gradually increasing. In this study, 4,211 pregnant women who underwent chromosomal microarray analysis(CMA) for high-risk indications were analyzed. The results showed that the overall prenatal detection rate of CMA was 11.4%(480/4211), among which the abnormal chromosome number was 5.82%(245/4211), copy number variants༈CNVs༉was 5.58%༈235/4211༉. Additionally, the detection rate of clinically significant copy number variants (CNVs) was 3.78% (235/4211) and 1.8% (76/4211) for variants of uncertain significance. The detection rate of abnormal chromosomes for pregnant women with AMA was 6.42%༈30/467༉, 6.01%༈50/832༉with high-risk MSS, 39.09%༈224/573༉with high-risk NIPT, 9.21%༈127/1379༉with abnormal ultrasound, and 5.1%༈49/960༉ with other indications. During follow-up, of the 4211 fetuses, 3677 fetuses (3677/4211,87.32%) were normal after birth, 462 fetuses (462/4211,10.97%) were terminated pregnancy, 51 (51/4211,1.21%) fetuses were abnormal after birth, and 21 (21/4211,0.50%) fetuses refused follow-up. These findings indicate that the diagnostic rate of CMA varies significantly among different indications, and can serve as a guide for clinicians to evaluate the application range of CMA technology in prenatal diagnosis.
Heart rate deceleration and acceleration capacities associated with circadian rhythm of blood pressure in essential hypertension
Authors: Wang Jijing , Xu Jinyi , Yang Lihong , Zhang You , Wu Rui , Wang Wentao , Gao Chuanyu
Published: January 2024

Background: This study aimed to investigate the potential association between the circadian rhythm of blood pressure and deceleration capacity (DC)/acceleration capacity (AC) in patients with essential hypertension.
Methods: This study included 318 patients with essential hypertension who underwent 24-hour ambulatory blood pressure monitoring. Patients were categorized into three groups based on the percentage of nocturnal systolic blood pressure (SBP) dipping: the dipper, non-dipper and reverse dipper groups. Baseline demographic characteristics, ambulatory blood pressure monitoring (ABPM) parameters, Holter recordings (including DC and AC), and echocardiographic parameters were collected.
Results: In this study, the lowest DC values were observed in the reverse dipper group, followed by the non-dipper and dipper groups (6.46±2.06 vs. 6.65±1.95 vs. 8.07±1.79 ms, P <.001). Additionally, the AC gradually decreased (-6.32±2.02 vs. -6.55±1.95 vs. -7.80±1.73 ms, P <.001). There was a significant association between DC (r=0.307, P<.001), AC (r=-0.303, P<.001) and nocturnal SBP decline. Furthermore, DC (β = 0.785, P =.001) was positively associated with nocturnal SBP decline, whereas AC was negatively associated with nocturnal SBP (β = -0.753, P<.002). By multivariate logistic regression analysis, deceleration capacity [OR (95% CI): 0.705 (0.594–0.836), p <.001], and acceleration capacity [OR (95% CI): 1.357 (1.141-1.614), p =.001] were identified as independent risk factors for BP nondipper status. The analysis of ROC curves revealed that the area under the curve for DC/AC in predicting the circadian rhythm of blood pressure was 0.711/0.697, with a sensitivity of 73.4%/65.1% and specificity of 66.7%/71.2%.
Conclusions: Abnormal DC and AC density were correlated with a blunted decline in nighttime SBP, suggesting a potential association between the circadian rhythm of blood pressure in essential hypertension patients and autonomic nervous dysfunction.
Clinical and Genetic Analysis of Li-Fraumeni Syndrome with Novel TP53 Mutations
Authors: Tian Yiping , Ma Zhengxiao , Wu Conghui , Chen Xu , Yue Zhuo , Ying Lisha , Su Dan
Published: January 2024

Objective
Mutations in the TP53 gene can cause Li-Fraumeni syndrome (LFS), an autosomal dominant genetic syndrome that increases susceptibility to various tumors. This study aims to explore the clinical and pathological features as well as the genetic characteristics of LFS to provide a theoretical basis for genetic counseling in affected families.
Methods
We conducted a retrospective analysis of clinical data and family history in three LFS cases with TP53 germline mutations. High-throughput sequencing technology was used to screen for hereditary tumor-related genes in the probands, and Sanger sequencing was used to confirm and analyze candidate pathogenic variant sites in their family members.
Results
Three different types of TP53 mutation variants were found in our study. The first family, spanning four generations and consisting of 30 individuals, included 9 adults diagnosed with 8 different types of cancer. Genetic testing revealed the TP53 c.642_643delTA p.H214Qfs*7 mutation in this family, showing that the age of onset tended to become younger in successive generations. The second family, with two patients having four different malignant tumors, carried the TP53 c.742C > T p.R248W mutation. This family had an average diagnosis age younger than the first family. The third proband, a 13-year-old boy, carried the TP53 c.844C > T p.R282W mutation and had no family history, indicating that this may be a new TP53 germline mutation in his family.
Conclusion
Our study identified and reported the pathogenic variant TP53 p.H214Qfs*7 frameshift mutation for the first time, expanding the mutation spectrum of the TP53 gene. We recommend timely genetic counseling and TP53 germline mutation testing for patients with childhood tumors or multiple familial tumors. Systematic monitoring of individuals carrying these mutations is crucial for early intervention to prevent primary and secondary tumors.
Ramsay-Hunt-Syndrome Associated with Unilateral Parotitis Following COVID-19 Vaccine
Authors: Soltanipur Masood , Yarmohammadi Hossein , Anari Seyed Fatah Mahdavi , Siadat Seyed Davar , Nemch Mohammadreza Karimi
Published: January 2024

Varicella-zoster-virus (VZV) reactivation is a recognized complication following COVID-19 vaccination; however, Ramsey-hunt-syndrome (RHS) rarely has been reported. This case presents a 36-year-old immunocompetent man with features of RHS three weeks following the second dose of the BBIBP-CorV (Sinopharm) vaccine. Interestingly, he developed acute unilateral parotitis on his left side and the ultrasonography showed a 55*44*18 mm parotid gland with decreased parenchymal echogenicity. He was treated with valacyclovir, and after seven days he was discharged. Also, his parotitis was resolved by day 29 following the presentation of the first symptoms with no further complications. The possibility of VZV reactivation after COVID-19 vaccination is an important point and it should be noted that this reactivation might present in unusual clinical scenarios.
Studies on new dithiophosphonic acid derivatives: Synthesis, characterization, DFT and molecular docking calculations and in vitro cytotoxicity assessment
Authors: Bulat Elif , Sağlam Ertuğrul Gazi , Akkoç Senem , Zeyrek Celal Tuğrul , Zorlu Yunus , Dal Hakan
Published: January 2024

2,4-bis(3,4-dimethoxyphenyl)-1,3-dithia-2,4-diphosphetane 2,4-disulfide (SAV-A1) was treated with five different alcohols to obtain dithiophosphonic acids (HLn, HS2P((2,4-CH3O)2-C6H3)(ORn); n=1-5, R1=n-pentyl-; R2=2-pentyl-; R3=4-tert-but-benzyl-; R4=2-propyl-; R5=2-butyl-). HLn series were converted to their ammonium salts, ([NH4Ln]). [NH4Ln] were reacted with NiCl2.6H2O to obtain the corresponding Ni(II) coordination compounds ([Ni(Ln)2]) in ethanol medium. The structures of all the compounds were elucidated by spectroscopic techniques. Single-crystal X-ray diffraction analysis of [Ni(L1)2], [Ni(L2)2], [Ni(L4)2] and [Ni(L5)2] were also carried out. The ligands and their nickel complexes were tested on human liver and colon cancer cell lines using (MTT) assay method. The salts [NH4L2] and [NH4L5] demonstrated more antiproliferative effects than others in colon cancer cell line with IC50 values 82.73 and 74.87 µM, respectively. Density Functional Theory (DFT) calculations of [Ni(L1)2], [Ni(L2)2], [Ni(L4)2] and [Ni(L5)2] were done. The molecular docking studies of [Ni(L1)2], [Ni(L2)2], [Ni(L4)2] and [Ni(L5)2] with colon cancer antigen proteins, ID 2HQ6 and liver cancer protein, PDB ID: 3WZE were done to foresee the interactions of the complexes.
Antibacterial and antibiofilm activities from soil Streptomyces spp. isolated from Muna Island, Indonesia against multidrug-resistant clinical isolates.
Authors: Prastya Muhammad Eka , Simbolon Sumihartati , Priyanto Jepri Agung , Hasidu La Ode Abdul Fajar , Permatasari Vera , Primahana Gian , Dewi Rizna Triana , Kristiana Rhesi , Suryanti Erma
Published: January 2024

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly been gaining concern and leading for investigating new strategies to tackle this problem. In this study, the antibacterial potential of 25 soil actinomycetes strain has been evaluated by initial screening against MDR bacterial strains including Escherichia coli strain M19, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M19, and Methicillin Resistant Staphylococcus aureus (MRSA). Among them, three actinomycetes isolates encoded APM-7, APM-11, and APM-21 exhibited strong and a broad antibacterial spectrum, hence there were selected for further study to extract its secondary metabolites following antibacterial, and antibiofilm assessment. The ethyl acetate extract of those three selected actinomycetes were evaluated for its antibacterial spectrum, and the minimum inhibitory concentration (MIC) ranged from 78 to 10000 µg/mL. Those extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract showed the best antibacterial and antibiofilm activities with the strongest values. Further, nucleotide sequencing of the 16S rRNA gene showed that these three potential strains APM-7, APM-11, and APM-21 to have identity with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Moreover, based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contains two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities recorded in this study. Ultimately, our study provides new insights into a promising candidate for use in an active compound combating strategy to prevent MDR bacterial strains infection.
Investigation on the biological activities of an amino alcohol ligand and its salicylaldehyde schiff base along with their copper complexes supported by structural, spectral, theoretical and docking studies
Authors: Jahangir Shirin , Mardani Zahra , Moeini Keyvan , Dušek Michal , Eigner Václav
Published: January 2024

Two complexes, [Cu(AEAE)Cl]Cl (1) and {[Cu2(L)2(µ3-NO3)]NO3}n (2), (AEAE: (2-((2-aminoethyl)amino)ethan-1-ol), HL: 2-(((2-((2-hydroxyethyl)amino)ethyl)imino)methyl)phenol), were prepared by template reaction (for 2) and identified by elemental analysis, FT-IR spectroscopy and single-crystal X-ray diffraction. The complex 1 adopts a mononuclear structure whereas 2 forms 1D coordination polymer. In the crystal structure of 1 the copper atom has a CuN2OCl environment with square planar geometry. In the coordination polymer of 2, there are two copper atoms with different geometries, octahedral (CuN2O4) and square-pyramidal (CuN2O3). In the crystal network of the complex 2, there are different types of the hydrogen bond motifs including R11(6), R11(8), R12(4), and R21(4). In addition to the hydrogen bonds there are π–π stacking interactions between phenyl rings. The charge distribution pattern of the complexes and their ligands was studied by NBO analysis and their ability to interact with ten biomacromolecules (BRAF kinase, CatB, DNA gyrase, HDAC7, rHA, RNR, TrxR, TS, Top II and B-DNA) is investigated by docking calculations and compared with that of doxorubicin.
Epidemiological characteristics of common respiratory pathogens in children
Authors: Lv Guojian , Shi Limei , Liu Yi , Sun Xuecheng , Mu Kai
Published: January 2024

Background:Children's respiratory tract infection is a common disease affecting children's health. Our aim is to describe the epidemiological characteristics of common pathogens of children's respiratory tract infection in outpatient clinics in central Shandong, China.
Methods: Sputum, tracheal aspirate, alveolar lavage fluid and other samples of 4804 children admitted to wards of Zibo Maternal and Child Health Hospital for treatment of respiratory tract infection from June 2019 to December 2022 were collected, and 12 common respiratory tract pathogens were detected by multiple PCR detection technology. Two bacteria (SP, HI), two atypical pathogens (MP, CP) and eight viruses (HRV, RSV, IVA, PIV, HMPV, HBOV, HCOV, IVB) were included. The proportion and positive detection rate of each type of respiratory tract mixed infection and the positive detection rate of single pathogen in different ages and seasons were statistically analyzed.
Results: (1) Among 4804 children with respiratory tract infection, the total positive rate was 77.87% (3741/4804). The positive rate was 34.47% (1656/4804). Streptococcus pneumoniae, rhinovirus and respiratory syncytial virus were the highest. There were 2085 cases of mixed infection with two or more pathogens, the positive rate was 43.40%. (2) The positive rates of infection in infant group (0-1 years old), infant group (1-3 years old), preschool group and school age group (3 years old -) were roughly the same. The infection rates of Streptococcus pneumoniae, respiratory syncytial virus and parainfluenza virus in infant group, rhinovirus in infant group, influenza A virus, chlamydia pneumoniae, mycoplasma pneumoniae and Haemophilus influenzae in school age group were higher than those in other groups. The difference was statistically significant (P<0.05). (3) The positive detection rates of spring, summer, autumn and winter groups were 43.58%, 38.64%, 33.73% and 29.27%, respectively. The positive rates of Streptococcus pneumoniae and Haemophilus influenzae in spring group, mycoplasma pneumoniae in summer group, rhinovirus, respiratory syncytial virus and influenza A virus in autumn group, chlamydia pneumoniae, Boca virus and influenza B virus in winter group were higher than those in other seasons, and the differences were statistically significant (P<0.05).
Conclusion：The pathogen detection rate of children varies with age and season, and the prevention and treatment of a certain respiratory pathogen infection must be combined with its raging season and age rule.
Drug-Related Problems and Clinical Pharmacist-Led Medication Therapy Management; Three-Year Survey in an Educational Pharmacotherapy Clinic
Authors: Valitabar Nastaran , Esmaily Hadi , Sistanizad Mohammad , Roshanzamiri Soheil , Farrokhian Amir , Mohammadnezhad Ghader
Published: January 2024

Objectives
Drug-related problems (DRPs) are adverse events or circumstances that interfere with favorable health outcomes. Medication therapy management (MTM) was developed to detect and manage DRP. This study set out to identify DRP-associated factors and reporting of subsequent interventions in a university-affiliated educational pharmacy.
Methods
This study was conducted from January 2020 to December 2022 at Shahid Kazemi Pharmacy, a large referral community pharmacy in Tehran, Iran. After medication review and complete medical history collection, DRPs were identified classified, and necessary interventions were performed. Medication adherence and satisfaction were evaluated before and 2–4 weeks after providing the MTM services.
Results
Within 1432 admissions, 838 patients were included and received 1116 interventions. Among the 1116 DRPs detected, the most common were drug information (27.4%), difficulty using dosage forms (26.3%), and any toxicity, allergic reaction, or adverse effects related to medications (12.8%). A significant association was detected between the number of DRPs and the age, the number of medications, and underlying chronic diseases (P < 0.05). Adjusting the age association of the number of medications and the number of underlying chronic diseases remained significant. The use of medications related to the alimentary tract and metabolism, medications affecting the blood and hematopoietic organs, medications affecting the cardiovascular system, antineoplastic and immunomodulatory medications, and medications affecting the nervous system were significantly associated with a higher risk for the occurrence of DRPs (P < 0.05).
Conclusions
Community pharmacy-led MTM services have a significant impact on DRP detection, management, medication adherence, and pharmacotherapy satisfaction.
Alterations in plasma lipid profile before and after surgical removal of soft tissue sarcoma
Authors: Lee Jae Hwa , Gwon Mi-Ri , Kim Jeung Il , Hwang Seung-young , Seong Sook Jin , Yoon Young-Ran , Kim Myungsoo , Kim Hyojeong
Published: January 2024

Background
Soft tissue sarcoma (STS) is a relatively rare malignancy, accounting for about 1% of all adult cancers. It is known to have more than 70 subtypes. Its rarity, coupled with its various subtypes, makes early diagnosis challenging. The current standard treatment for STS is surgical removal. To aid in identifying prognosis and pathogenesis, we utilized an untargeted metabolomic approach to profile the altered endogenous metabolites in pre-operative and post-operative plasma samples of STS patients.
Methods
We collected pre-operative and post-operative plasma samples from 24 patients with STS who underwent surgical removal of masses. Plasma metabolic profiling was conducted using ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry. Out of the 24 patients, 11 experienced recurrences after the operations. Multivariate analysis and permutation tests were conducted to identify putative altered metabolites. Univariate receiver operator characteristic analysis was performed to evaluate their predictive performance.
Results
Thirty-nine putative metabolites were identified based on the orthogonal projections to latent structures-discriminant analysis, with 34 of them showing statistical significance. These metabolites included phospholipids and acyl-carnitines, indicating changes in lipid metabolism. Specifically, phospholipids exhibited an increase in the post-operative samples, while acyl-carnitines showed a decrease. Notably, lysophosphatidylcholine (LPC) O-18:0 and LPC-O16:2 demonstrated predictive capabilities for STS recurrence, with area under the curve values of 0.748 and 0.797, respectively.
Conclusions
Our investigation revealed distinct alterations in the lipid profiles of plasma in STS patients after surgical resection of masses. We anticipate that these findings can contribute to the elucidation of the pathophysiology of STS and the development of further metabolic studies in this rare malignancy.
UGT1A1 variants contribution to neonatal hyperbilirubinemia: evidence from whole exome sequencing Running title: UGT1A1 variants contribution to neonatal hyperbilirubinemia
Authors: Liao Yu-Wei , Lin Fen , Xu Jia-Xin , Yang Hui , Wu Yong-Hao , Chen Man-Tong , Ma Yu-Bin , Li Jian-Dong , Yang Li-Ye
Published: January 2024

Background
It is widely recognized that genetic variation is among the risk factors associated with neonatal jaundice. Therefore, the objective of this study was to assess the impact of genetic variants on Chinese neonates with hyperbilirubinemia.
Methods
This cohort consisted of 43 cases with normal bilirubin levels, 54 cases categorized as the medium jaundice group (total serum bilirubin less than 342 umol/L), and 33 cases belonging to the high jaundice group (total serum bilirubin exceeding 342 umol/L). Whole exome sequencing was performed on the neonates to identify genetic variations.
Results
UGT1A1-G71R, mixed feeding and breast feeding were significantly correlated with higher bilirubin value. Homozygous SNP of CAPN12, ECT2L, SLC9B2, PLEKHA4, and hemizygous G6PD were only found in jaundice group but not in control group. Consistent with the result of linear regression, feeding pattern and UGT1A1 G71R were all correlated with level of bilirubin in Fisher exact test (p = 7.81E-13, and p = 2.48E-07, respectively).
Conclusion
The UGT1A1 gene variants emerged as a prominent risk factor for neonatal jaundice, significantly contributing to elevated bilirubin levels in Chinese neonates, particularly in conjunction with breastfeeding.
Development and validation of inpatient mortality prediction models for patients with hyperglycemic crisis using machine learning approaches
Authors: He Rui , Zhang Kebiao , Li Hong , Gu Manping
Published: January 2024

Background：Hyperglycemic crisis is one of the most common complications of diabetes mellitus with a high motarlity rate. Emergency admissions for hyperglycemic crisis are still very common and challenging. The study aimed to develop and validate models for predicting the inpatient mortality risk of patients with hyperglycemic crisis admitted in emergency department using different machine learning(ML) methods.
Methods: We carried out a multi-center retrospective study within six large general adult hospitals in Chongqing, western China. Patients diagnosed with hyperglycemic crisis were included based on an electronic medical record (EMR) database. The patients’ medical records along with demographics, comorbidities, clinical characteristics, laboratory results, complications, and therapeutic measures were extracted to construct theprognostic prediction model. We applied seven machine learning algorithms (support vector machines (SVM), random forest (RF), recursive partitioning and regression trees (RPART), extreme gradient boosting with dart booster (XGBoost), multivariate adaptive regression splines (MARS), neural network (NNET), and adaptive boost (AdaBoost)) compared with logistic regression (LR) to predict the risk of in-hospital death in patients with hyperglycemic crisis. Stratified random sampling was used to split the data into training (80%) and validation (20%) sets. Ten-fold cross validation was performed in train to optimize model hyper-parameters. The sensitivity, specificity, positive and negative predictive values, area under the curve (AUC) and accuracy of all models were computed in order to compare them.
Results: A total of 1668 patients were eligible for the present study. The mortality rate during hospitalization was 7.3%(121/1668). In the training set, we calculated importance scores for each feature for eight models, and themost significant 10 features for all models were listed. In the validation set, all models showed good predictive capability with areas under the curve above 0.9 except the MARS model. Six machine learning algorithm models outperformed the referred logistic regression algorithm except the MARS model. RPART, RF, and SVM have better performance in the selected models (AUC values were 0.970, 0.968 and 0.968, respectively). Variable importance revealed newly detected predictors including mechanical ventilation, age, Charlson Comorbidity Index, blood gas index, first 24-hour insulin dosage, and first 24-hour fluid intake.
Conclusion: All machine learning algorithms performed well to predict inpatient mortality in patients with hyperglycemic crisis except the MARS model, and the best was RPART model. These algorithms identified overlapping but different, up to 10 predictors. These models identify high-risk patients early, can assist in clinical decision-making, and can improve the prognosis of hyperglycemic crisis patients to some extent.
A Novel Method Of Specific Adsorption Of Hazardous Hydrogen Sulfide Gas in Gas Processes By Core-Shell Hybrid Nano Adsorbent MIL-101(Cr)@MIPs@H2S
Authors: Moradirad Rohadin , Mahabadi Hassan Asilian , Shahtaheri Seyed Jamaleddin , Rashidi Alimorad , Fakhraie Saeed
Published: January 2024

Hydrogen sulfide gas is a challenge in the oil and gas industry due to its toxicity and corrosive nature. In addition to the toxicity of H2S, it is very corrosive both in gas and when dissolved. It is oxidized when oxidized in the combustion process, and the form of SO2 causes acid rain. Adsorption using MIL-101(Cr) @NIPs/MIPs@H2S adsorbents offers a promising solution with high efficiency, low cost, and low energy consumption. This study optimized the operating parameters for efficient and cost-effective adsorption of H2S gas. Thirty experiments were conducted to analyze the impact of the operating parameters of the adsorbent (0.1 to 1 g), temperature (25 to 80°C), concentration (10 to 1000 ppm), and flow rate (40 to 100 mL/min) on the process. The evaluation was carried out to determine cause-and-effect relationships between the variables above. The molar adsorption capacity of gases in a 400 mm high, 10 mm internal diameter fixed bed was determined using the central composite design method and the Soave-Redlich-Kwong equation. FTIR, XRD, FE-SEM, and BET techniques were then used to determine the physical properties. The statistical analysis of variance results indicated that the adsorbents adhere to the quadratic model, with temperature and adsorbent dose being the primary process variables. The optimum adsorption efficiency and capacity for MIL-101(Cr)@MIPs@H2S (11 mg/g = 94.3%) is higher than that of MIL-101(Cr)@NIPs@H2S (5.97 mg/g = 9.9%) and due to a better match between the template and binding sites in the template layer, which facilitates efficient template uptake and removal. The equilibrium adsorption of all Nano-adsorbents followed the pseudo-Scott order and Langmuir isotherm models. MIL-101(Cr) @MIPs@H2S proved to be a reliable and stable adsorbent for hydrogen sulfide. Its specific adsorption selectivity for H2S resulted in a significantly higher adsorption capacity than other gases in mixed CO2 and CH4.