bluuebunny/crossref_metadata_2025 huggingface dataset:
Individual Differences in Parental Support for Numeracy and Literacy in Early Childhood
Authors: Elliott Leanne , Zheng Peter , Libertus Melissa
Published: September 2021

Past research has examined parental support for children’s math and reading skills in the early years through parents’ reports of their activities with their children in somewhat inconsistent ways. In this study, we use data from a large sample of parents (n = 259; 103 males) collected through Amazon’s Mechanical Turk to examine dimensions of parental enrichment in both support for literacy and numeracy skills at home. Additionally, we examine how socioeconomic resources as well as parental beliefs relate to these dimensions of the home literacy and home numeracy environment. Factor analyses revealed two dimensions of literacy activities (i.e., passive and active literacy activities) and three dimensions of numeracy activities (i.e., numeracy applications, basic numeracy, and written numeracy activities). Income was positively associated with active literacy activities, whereas parents’ educational attainment was negatively associated with active literacy activities and written numeracy activities. Additionally, parental beliefs, including their beliefs about the importance of literacy and math skills as well as their perceived responsibility for teaching their children reading, math, and language skills, related to home literacy and numeracy activities in distinctive ways. These results suggest that future research should explore parental enrichment practices with greater nuance, particularly when examining associations with socioeconomic status.
A Chopper-Embedded BGR Composite Noise Reduction Circuit for Clock Generator
Authors: Agarwal Neeru , Agarwal Neeraj , Lu Chih-Wen , Oh-e Masahito
Published: September 2021

A chopper-embedded bandgap reference (BGR) scheme is presented using 0.18 μm CMOS technology for low-frequency noise suppression in the clock generator application. As biasing circuitry produces significant flicker noise, along with thermal noise from passive components, the proposed low-noise chopper-stabilized BGR circuit was designed and implemented for a wide range of temperature from −40 to 125 °C, including a startup and self-biasing circuit to reduce critical low-frequency noise from the bias circuitry and op amp input offset voltage. The BGR circuit generated a reference voltage of 1.25 V for a supply voltage range of 2.5–3.3 V. The gain of the implemented BGR operational transconductance amplifier is 84.1 dB. A non-overlapping clock circuit was implemented to reduce the clock skew effect, which is also one of the noise contributors. The noise analysis of a chopped bandgap voltage reference was evaluated through cadence periodic steady-state (PSS) analysis and periodic noise (PNoise) analysis. The low-frequency flicker noise was reduced from 1.5 to 0.4 μV/sqrt(Hz) at 1 KHz, with the proposed chopping scheme in the bandgap. Comparisons of the noise performance of the chopper-embedded BGR, with and without a low-pass filter, were also performed, and the results show a further reduction in the overall noise. A reduction in the flicker noise, from 181.3 to 10.26 mV/sqrt(Hz) at 100 KHz, was observed with the filter. All circuit blocks of the proposed BGR scheme were designed and simulated using the EDA tool HSPICE, and layout generation was carried out by Laker. The BGR architecture layout dimensions are 285.25 μm × 125.38 μm.
Manual Frequency Restoration Reserve Activation Clearing Model
Authors: Roumkos Christos , Biskas Pandelis N. , Marneris Ilias
Published: September 2021

The integration of the European markets has started with the successful coupling of spot markets (day-ahead and intra-day) and is expected to continue with the coupling of balancing markets. In this paper, the optimization model for the activation of manual frequency restoration reserve (mFRR) is presented. The model incorporates all order types agreed among the European transmission system operators (TSOs) to be included in the Manually Activated Reserves Initiative (MARI) project. Additionally, the model incorporates the buying curve (demand) of mFRR with the possible tolerance band defined by the TSOs, order clearing constraints and the cross-zonal capacity (CZC) constraints, forming a mixed integer linear programming model. The methodology employs two distinct steps: In the first step, an order conversion process is employed for the markets applying the central-scheduling scheme, and in the second step, the mFRR activation process is executed by solving the presented model. The whole process is tested using a case, including twenty-five European control areas. The attained clearing results indicate that price convergence is achieved among the involved control areas, along with a reduction in the overall balancing costs mainly due to the imbalance netting that is implicitly performed during the joint mFRR balancing energy (BE) clearing process and due to the cross-border exchange of mFRR BE.
Investigation of Optimal Hybrid Energy Systems Using Available Energy Sources in a Rural Area of Bangladesh
Authors: Rashid Fazlur , Hoque Md. Emdadul , Aziz Muhammad , Sakib Talukdar Nazmus , Islam Md. Tariqul , Robin Raihan Moker
Published: September 2021

The aims of this paper are to develop hybrid energy systems considering biomass energy sources as well as a framework and optimal configuration of hybrid systems of energy for a southern sub-urban area of Bhola district in Bangladesh, named Kukri Mukri island, and analyse the feasibility of the techno-economic prospects of these systems. In this work, electrification for the rural area is analysed for different configurations of the hybrid systems. The estimation of available resources with optimal sizing and analysis of techno-economic aspects is done through HOMER Pro software to satisfy the demand of peak load. Different configurations of hybrid energy systems, including PV/diesel, PV/wind, PV/diesel/wind, PV/wind/diesel/biomass, and wind/diesel, are analysed and compared through optimization of different energy sources in HOMER. The size of the system and components are optimized and designed depending on the net present cost (NPC) and the levelized cost of energy (LCOE). Due to the lower availability and rising cost of wind energy, the outcome of this work shows a solar-based photovoltaic (PV) as the main energy source, battery as the storage media, and diesel generator as an energy source for backup. The results indicate that LCOE is much lower for PV/wind/diesel/biomass (0.142 USD/kWh) than PV/diesel (0.199 USD/kWh), PV/wind (0.239 USD/kWh), PV/diesel/wind (0.167 USD/kWh), PV/diesel (0.343 USD/kWh), and wind/diesel (0.175 USD/kWh). Additionally, it is demonstrated from the research that the genetic algorithm (GA) process gives sustainable and cost-effective outcomes compared to HOMER.
Harmonic Transfers for Quantifying Propagation of Harmonics in Wind Power Plants
Authors: Schwanz Daphne , Bollen Math , Lennerhag Oscar , Larsson Anders
Published: September 2021

In this paper, primary and secondary emissions in wind power plants are studied by using transfer admittance and current transfer functions between turbines and the public grid. The use of such transfer functions allows harmonic propagation studies without knowledge of the emission from individual turbines or the background voltage distortion. The transfer functions are calculated for one synthetic and one existing wind power plant, and results are discussed. Primary emission, secondary emission from other turbines and secondary emission from the public grid are shown to be of the same order of magnitude. Furthermore, the paper addresses the impact of turbine converter modelling, public grid impedance and the change in the aggregation exponent with frequency on the propagation. All three are shown to have a significant impact and should be considered. The main challenge for future studies is in obtaining relevant models for turbine impedance versus frequency.
Continuous Support for Roadways
Authors: Mazurek Krzysztof , Szyguła Marek , Figiel Andrzej , Filipowicz Krzysztof
Published: September 2021

Opening deeper coal seams requires constructing underground mine roadways in difficult geological conditions. Supporting of such roadways is subjected to a very high load from the rock mass. The types of roof supports used so far do not provide immediate support for the rock mass, which tends to converge the roadway, allowing for a rapid build-up of stresses in the surrounding rock mass. The article presents a new type of frame roadway support. This is a yielding support (consecutive arches are connected in a helical pattern), enabling the successive arches to be provided with initial load-bearing capacity already at the construction stage. The so-called unscrewing of the helix enables the arches to be pressed against the surface of the developed roadway with a controlled force. The introduction discusses the types of yielding roof supports used in the Polish mining industry and indicates their characteristic features. Further along in the article, the assumptions adopted for the construction of models to be tested and assumptions for the static and dynamic load to the models are defined, and the results of the model numerical tests are presented. The tests were aimed at comparing the qualitative behavior of the new roof support and the closed, circular support which is closest to it. The results of numerical tests confirmed the strength of the new solution not lower than the closed (circular) frame support, previously used in the most difficult geological conditions.
Precise Measurements of the Temperature Influence on the Complex Permittivity of Power Transformers Moistened Paper-Oil Insulation
Authors: Zukowski Pawel , Rogalski Przemyslaw , Kierczynski Konrad , Koltunowicz Tomasz N.
Published: September 2021

The reference characteristics of complex permittivity of the transformers insulation solid component were determined for use in the precise diagnostics of the power transformers insulation state. The solid component is a composite of cellulose, insulating oil and water nanoparticles. Measurements were made in the frequency range from 10−4 Hz to 5000 Hz at temperatures from 293.15 to 333.15 K. Uncertainty of temperature measurements was less than ±0.01 K. Pressboard impregnated with insulating oil with a water content of (5.0 ± 0.2) by weight moistened in a manner maximally similar to the moistening process in power transformers was investigated. It was found that there are two stages of changes in permittivity and imaginary permittivity components, occurring for low and high frequency. As the temperature increases, the frequency dependencies of the permittivity and imaginary permittivity component shifts to the higher frequency region. This phenomenon is related to the change of relaxation time with the increase in temperature. The values of relaxation time activation energies of the permittivity ΔWτε′ ≈ (0.827 ± 0.0094) eV and the imaginary permittivity component ΔWτε″ = 0.883 eV were determined. It was found that Cole-Cole charts for the first stage are asymmetric and similar to those described by the Dawidson–Cole relaxation. For stage two, the charts are arc-shaped, corresponding to the Cole-Cole relaxation. It has been established that in the moistened pressboard impregnated with insulating oil, there is an additional polarization mechanism associated with the occurrence of water in the form of nanodrops and the tunneling of electrons between them.
Optimal Placement and Operation of Chlorine Booster Stations: A Multi-Level Optimization Approach
Authors: Pineda Sandoval Joseph D. , Brentan Bruno Melo , Lima Gustavo Meirelles , Cervantes Daniel Hernández , García Cervantes Daniel A. , Ramos Helena M. , Delgado Galván Xitlali , Mora Rodríguez José de Jesús
Published: September 2021

Chlorine demand as a disinfectant for water utility impacts on unintended energy consumption from electrolysis manufacture; thus, diminishing the chlorine consumption also reduces the environmental impact and energy consumption. Problems of disinfectant distribution and uniformity in Water Distribution Networks (WDN) are associated with the exponential urban growth and the physical and biochemical difficulties within the network. This study optimizes Chlorine Booster Stations (CBS) location on a network with two main objectives; (1) to deliver minimal Free Residual Chlorine (FRC) throughout all demand nodes according to country regulations, and (2) to reduce day chlorine mass concentration supplied in the system by applying an hour time pattern in CBS, consequently associated economic, energy and environmental impacts complying with regulatory standards. The application is demonstrated on a real-world WDN modeled from Guanajuato, Mexico. The resulting optimal location and disinfectant dosage schedule in CBS provided insights on maintaining disinfectant residuals throughout all the WDN to prevent health issues and diminishing chlorine consumption.
Effect of Lignite Properties on Its Suitability for the Implementation of Underground Coal Gasification (UCG) in Selected Deposits
Authors: Kapusta Krzysztof
Published: September 2021

Two experimental simulations of underground coal gasification (UCG) processes, using large bulk samples of lignites, were conducted in a surface laboratory setup. Two different lignite samples were used for the oxygen-blown experiments, i.e., “Velenje” meta-lignite (Slovenia) and “Oltenia” ortho-lignite (Romania). The average moisture content of the samples was 31.6wt.% and 45.6wt.% for the Velenje and Oltenia samples, respectively. The main aim of the study was to assess the suitability of the tested lignites for the underground coal gasification process. The gas composition and its production rates, as well as the temperatures in the artificial seams, were continuously monitored during the experiments. The average calorific value of gas produced during the Velenje lignite experiment (6.4 MJ/Nm3) was much higher compared to the result obtained for the experiment with Oltenia lignite (4.8 MJ/Nm3). The Velenje lignite test was also characterized by significantly higher energy efficiency, i.e., 44.6%, compared to the gasification of Oltenia lignite (33.4%). The gasification experiments carried out showed that the physicochemical properties of the lignite used considerably affect the in situ gasification process. Research also indicates that UCG can be considered as a viable option for the extraction of lignite deposits; however, lignites with a lower moisture content and higher energy density are preferred, due to their much higher process efficiency.
Load Sharing Scheme Incorporating Power Security Margins for Parallel Operation of Voltage Source Inverters
Authors: Eyisi Chiebuka , Li Qifeng
Published: September 2021

The interconnection of distributed energy resources (DERs) in microgrids (MGs) operating in both islanded and grid-connected modes require coordinated control strategies. DERs are interfaced with voltage source inverters (VSIs) enabling interconnection. This paper proposes a load demand sharing scheme for the parallel operation of VSIs in an islanded voltage source inverter-based microgrid (VSI-MG). The ride-through capability of a heavily loaded VSI-MG, where some of the VSIs are fully loaded due to the occurrence of an event is investigated. In developing analytical equations to model the VSI, the concept of virtual synchronous machines (VSM) is applied to enable the VSI mimic the inertia effect of synchronous machines. A power frame transformation (PFT) that takes the line ratios of the MG network into account is also incorporated to yield satisfactory transient responses of both network frequency and bus voltages in the MG network. A Jacobian-based method is then developed to take into account the operational capacity of each VSI in the VSI-MG. The resulting amendable droop control constrains the VSIs within their power capabilities when an event occurs. Simulation results presented within demonstrate the effectiveness of the proposed procedure which has great potential to facilitate efforts in maintaining system reliability and resiliency.
Public Acceptance and Support of Renewable Energy in the North-East Development Region of Romania
Authors: Jijie Dumitru-Tudor , Maxim Alexandru , Roman Teodora , Roșcovan Mihail
Published: September 2021

Concern about energy in the European Union (EU) has been a recurrent issue from the very beginning. Though initially addressed at the state level, energy is now a shared competency as stressed by article 194 of the Treaty on the Functioning of the European Union. New challenges, added from time to time, need public support in order to be properly addressed. Such is the case of substituting traditional energy production with renewable energy sources. Our paper seeks to determine whether the Romanian public opinion favors such an evolution, which is traditionally associated with significant investment efforts. The study is focused on the north-east development region, which has the highest population and registered the fastest economic growth in 2019. The topic was explored through a survey applied to a sample of 649 household respondents. The results suggest strong support for introducing renewable energy sources, serious concerns about climate change, and a preoccupation for energy saving. Concerns regarding climate change or various economic factors, behaviors oriented towards reducing energy waste, as well as perceived knowledge on the matter are the factors with the biggest impact on supporting electricity production based on renewable energy sources. However, TV and online exposure have a negative impact on support. Demographics, along with social and political values remain mostly not significant.
Environmental Assessment and Regulatory Aspects of Cold Ironing Planning for a Maritime Route in the Adriatic Sea
Authors: Piccoli Tobia , Fermeglia Matteo , Bosich Daniele , Bevilacqua Paolo , Sulligoi Giorgio
Published: September 2021

The technology of cold ironing (or shore-to-ship power) can meaningfully reduce greenhouse gases and air pollutant emissions from ships at the berth by powering the vessels from the electrical shore grid. While cold ironing constitutes an effective and affordable solution in northern Europe and America, economic, legal, and environmental factors still render this technology less attractive in southern Europe. This paper aims to unpack and analyze the economic, regulatory, and environmental factors that can foster cold ironing as a standard installation in the Mediterranean Sea. Based on a model design for the port of Trieste (Italy) as applied to a cluster of target ports in the Adriatic Sea (in Italy, Croatia, and Greece), this article evaluates the cold ironing payback period by comparing costs of shore side-plants with environmental externalities and O&M costs. Moreover, the paper addresses key regulatory bottlenecks arising in different European jurisdictions with regard to the setting-up and development of cold ironing, while appraising the legal and economic consequences of deploying cold ironing in light of the future inclusion of the maritime sector in the EU Emission Trading System.
On the Mathematical Modelling of a Moving-Bed Counter-Current Gasifier Fuelled with Wood-Pellets
Authors: Schwabauer Andreas , Mancini Marco , Poyraz Yunus , Weber Roman
Published: September 2021

The subject of this work is the mathematical modelling of a counter-current moving-bed gasifier fuelled by wood-pellets. Two versions of the model have been developed: the one-dimensional (1D) version-solving a set of Ordinary Differential Equations along the gasifier height-and the three-dimensional (3D) version where the balanced equations are solved using Computational Fluid Dynamics. Unique procedures have been developed to provide unconditionally stable solutions and remove difficulties occurring by using conventional numerical methods for modelling counter-current reactors.The procedures reduce the uncertainties introduced by other mathematical approaches, and they open up the possibility of straightforward application to more complex software, including commercial CFD packages. Previous models of Hobbs et al., Di Blasi and Mandl et al. used a correction factor to tune calculated temperatures to measured values. In this work, the factor is not required. Using the 1D model, the Mandl et al. 16.6 kW gasifier was scaled to 9.5 MW input; the 89% cold-gas efficiency, observed at 16.6 kW input, decreases only slightly to 84% at the 9.5 MW scale.
Data-Driven Prognostics of the SOFC System Based on Dynamic Neural Network Models
Authors: Cheng Shan-Jen , Li Wen-Ken , Chang Te-Jen , Hsu Chang-Hung
Published: September 2021

Prognostics technology is important for the sustainability of solid oxide fuel cell (SOFC) system commercialization, i.e., through failure prevention, reliability assessment, and the remaining useful life (RUL) estimation. To solve SOFC system issues, data-driven prognostics methods based on the dynamic neural network (DNN), one of non-linear models, were investigated in this study. Based on DNN model types, the neural network autoregressive (NNARX) model with external inputs, the neural network autoregressive moving average (NNARMAX) model with external inputs, and the neural network output error (NNOE) were utilized to predict the degradation trend and estimate the RUL. First, the degradation trend prediction was executed to evaluate the correctness of the proposed DNN model structures in the first learning phase. Then, the RUL was estimated on the basis of the degradation trend of the NN models in the second inference phase. The comparison test results show the prediction accuracy of the NNARX model is higher and the RUL estimation can be given within a smaller relative error than the NNARMAX and NNOE models. The evaluation criteria of the root mean square error and mean absolute error of the NNARX model are the smallest among these three models. Therefore, the proposed NNARX model can effectively and precisely provide degradation trend prediction and RUL estimation of the SOFC system.
Asymmetrical Order in Wall-Bounded Turbulent Flows
Authors: Lee T.-W.
Published: September 2021

Scaling of turbulent wall-bounded flows is revealed in the gradient structures, for each of the Reynolds stress components. Within the “dissipation” structure, an asymmetrical order exists, which we can deploy to unify the scaling and transport dynamics within and across these flows. There are subtle differences in the outer boundary conditions between channel and flat-plate boundary-layer flows, which modify the turbulence structure far from the wall. The self-similarity exhibited in the gradient space and corresponding transport dynamics establish capabilities and encompassing knowledge of wall-bounded turbulent flows.
An Extended Model of the Theory of Planned Behaviour to Predict Local Wine Consumption Intention and Behaviour
Authors: Sabina del Castillo Edgar J. , Díaz Armas Ricardo J. , Gutiérrez Taño Desiderio
Published: September 2021

The consumption of local agricultural products boosts the regional economy and employment whilst preserving the rural landscape and environment. In this research, the background of local wine consumption behaviour will be studied, using an extended model of the Theory of Planned Behaviour. Partial least squares structural equation modelling (PLS–SEM) was used to test the hypotheses. The study was conducted in the Canary Islands with a sample of 762 people. The results confirmed a relationship between intention and perceived behavioural control. Furthermore, the ethnocentric personality was found to have a positive influence and the cosmopolitan personality a negative influence. The personal norm and place identity were also confirmed to be related to attitudes towards such behaviour. This study contributes to the literature by adding constructs to this theory that are relevant to local wine consumption. It also addresses the implications for those involved in the marketing of local products.
Enhancement in Thermal Energy and Solute Particles Using Hybrid Nanoparticles by Engaging Activation Energy and Chemical Reaction over a Parabolic Surface via Finite Element Approach
Authors: Chu Yu-Ming , Nazir Umar , Sohail Muhammad , Selim Mahmoud , Lee Jung-Rye
Published: September 2021

Several mechanisms in industrial use have significant applications in thermal transportation. The inclusion of hybrid nanoparticles in different mixtures has been studied extensively by researchers due to their wide applications. This report discusses the flow of Powell–Eyring fluid mixed with hybrid nanoparticles over a melting parabolic stretched surface. Flow rheology expressions have been derived under boundary layer theory. Afterwards, similarity transformation has been applied to convert PDEs into associated ODEs. These transformed ODEs have been solved the using finite element procedure (FEP) in the symbolic computational package MAPLE 18.0. The applicability and effectiveness of FEM are presented by addressing grid independent analysis. The reliability of FEM is presented by computing the surface drag force and heat transportation coefficient. The used methodology is highly effective and it can be easily implemented in MAPLE 18.0 for other highly nonlinear problems. It is observed that the thermal profile varies directly with the magnetic parameter, and the opposite trend is recorded for the Prandtl number.
CMOS OTA-Based Filters for Designing Fractional-Order Chaotic Oscillators
Authors: Valencia-Ponce Martín Alejandro , Castañeda-Aviña Perla Rubí , Tlelo-Cuautle Esteban , Carbajal-Gómez Victor Hugo , González-Díaz Victor Rodolfo , Sandoval-Ibarra Yuma , Nuñez-Perez Jose-Cruz
Published: September 2021

Fractional-order chaotic oscillators (FOCOs) have shown more complexity than integer-order chaotic ones. However, the majority of electronic implementations were performed using embedded systems; compared to analog implementations, they require huge hardware resources to approximate the solution of the fractional-order derivatives. In this manner, we propose the design of FOCOs using fractional-order integrators based on operational transconductance amplifiers (OTAs). The case study shows the implementation of FOCOs by cascading first-order OTA-based filters designed with complementary metal-oxide-semiconductor (CMOS) technology. The OTAs have programmable transconductance, and the robustness of the fractional-order integrator is verified by performing process, voltage and temperature variations as well as Monte Carlo analyses for a CMOS technology of 180 nm from the United Microelectronics Corporation. Finally, it is highlighted that post-layout simulations are in good agreement with the simulations of the mathematical model of the FOCO.
Thermally Tunable Acoustic Beam Splitter Based on Poly(vinyl alcohol) Poly(N-isopropylacrylamide) Hydrogel
Authors: Jin Yuqi , Zhou Mi , Choi Tae-Youl , Neogi Arup
Published: September 2021

In this study, we demonstrated a thermally tunable acoustic beam splitter using a poly(vinyl alcohol) poly(N-isopropylacrylamide) hydrogel (PVA-pNIPAM). The nature of PVA-pNIPAM hydrogel offers exceptional temperature-dependent physical properties due to its phase transition around its lower critical solution temperature. The acoustic impedance of the hydrogel can be tuned below, above, or matched to that of water by changing the environmental temperature. An acoustic wave propagating in water can be split into transmitted and reflected components by the PVA-pNIPAM hydrogel slab on varying its angle of incidence. The intensity ratio between the reflected and the transmitted componence can be adjusted by tuning the temperature of the medium. The acoustic beam can be entirely reflected at a temperature corresponding to the matched impedance between hydrogel and water. The beam-splitting behavior was observed for acoustic waves from both a monochromatic wave and broadband pulse source. In addition, the phase of beam split pulses can be reversed by selecting the hydrogel’s operating temperature.
Predict Health Care Accessibility for Texas Medicaid Gap
Authors: Zhang Jinting , Wu Xiu
Published: September 2021

Medicaid is a unique approach in ensuring the below poverty population obtains free insurance coverage under federal and state provisions in the United States. Twelve states without expanded Medicaid caused two million people who were under the poverty line into health insecurity. Principal Component-based logistical regression (PCA-LA) is used to consider health status (HS) as a dependent variable and fourteen social-economic indexes as independent variables. Four composite components incorporated health conditions (i.e., “no regular source of care” (NRC), “last check-up more than a year ago” (LCT)), demographic impacts (i.e., four categorized adults (AS)), education (ED), and marital status (MS). Compared to the unadjusted LA, direct adjusted LA, and PCA-unadjusted LA three methods, the PCA-LA approach exhibited objective and reasonable outcomes in presenting an odd ratio (OR). They included that health condition is positively significant to HS due to beyond one OR, and negatively significant to ED, AS, and MS. This paper provided quantitative evidence for the Medicaid gap in Texas to extend Medicaid, exposed healthcare geographical inequity, offered a sight for the Centers for Disease Control and Prevention (CDC) to improve the Medicaid program and make political justice for the Medicaid gap.
Biological Contribution of Ornamental Plants for Improving Slope Stability along Urban and Suburban Areas
Authors: Francini Alessandra , Toscano Stefania , Romano Daniela , Ferrini Francesco , Ferrante Antonio
Published: September 2021

Plants can reduce erosion during heavy raining periods and improve slope stability through their root morphology, development, biomass, and architecture. Heavy rains can increase erosion, becoming a danger for traffic and people who live around slopes. The control of slope stability is often required in urban and peri-urban environments, and for this reason ornamental species can be appropriately selected for a dual use, namely improving the aesthetical value of green areas along the urban and suburban roads and mitigating the erosion effects. The species used must have good tolerance to abiotic stresses, such as high and low temperature, drought, pollution and nutrient deficiency. Otherwise, their limited growth can reduce their beneficial effects. Ornamental plants that can be used for reducing the erosion of slopes must be in full growth during periods with a higher incidence of rains and must also be compatible with the temperature ranges in different seasons. These species can be also selected for their ability to avoid erosion and enhance the stability of slopes. In this review, the biological contribution of plants for improving slope stability has been reported and discussed with a special focus attention on the Mediterranean environment. Particular emphasis has been placed on root biomass changes and root growth parameters, considering their role as potential markers for selecting suitable plants to be used for enhancing slope stability. A brief description of planting on slopes and root growth has been also considered and discussed.
Geospatial Data Utilisation in National Disaster Management Frameworks and the Priorities of Multilateral Disaster Management Frameworks: Case Studies of India and Bulgaria
Authors: Ghawana Tarun , Pashova Lyubka , Zlatanova Sisi
Published: September 2021

Facing the increased frequency of disasters and resulting in massive damages, many countries have developed their frameworks for Disaster Risk Management (DRM). However, these frameworks may differ concerning legal, policy, planning and organisational arrangements. We argue that geospatial data is a crucial binding element in each national framework for different stages of the disaster management cycle. The multilateral DRM frameworks, like the Sendai Framework 2015–2030 and the United Nations Committee of Experts on Global Geospatial Information Management (UNGGIM) Strategic Framework on Geospatial Information and Services for Disasters, provide the strategic direction, but they are too generic to compare geospatial data in national DRM frameworks. This study investigates the two frameworks and suggests criteria for evaluating the utilisation of geospatial data for DRM. The derived criteria are validated for the comparative analysis of India and Bulgaria’s National Disaster Management Frameworks. The validation proves that the criteria can be used for a general comparison across national DRM.
Ultra-Low Frequency Transcutaneous Electrical Nerve Stimulation on Pain Modulation in a Rat Model with Myogenous Temporomandibular Dysfunction
Authors: Hsieh Yueh-Ling , Yang Chen-Chia , Yang Nian-Pu
Published: September 2021

Masticatory myofascial pain (MMP) is one of the most common causes of chronic orofacial pain in patients with temporomandibular disorders. To explore the antinociceptive effects of ultra-low frequency transcutaneous electrical nerve stimulation (ULF-TENS) on alterations of pain-related biochemicals, electrophysiology and jaw-opening movement in an animal model with MMP, a total of 40 rats were randomly and equally assigned to four groups; i.e., animals with MMP receiving either ULF-TENS or sham treatment, as well as those with sham-MMP receiving either ULF-TENS or sham treatment. MMP was induced by electrically stimulated repetitive tetanic contraction of masticatory muscle for 14 days. ULF-TENS was then performed at myofascial trigger points of masticatory muscles for seven days. Measurable outcomes included maximum jaw-opening distance, prevalence of endplate noise (EPN), and immunohistochemistry for substance P (SP) and μ-opiate receptors (MOR) in parabrachial nucleus and c-Fos in rostral ventromedial medulla. There were significant improvements in maximum jaw-opening distance and EPN prevalence after ULF-TENS in animals with MMP. ULF-TENS also significantly reduced SP overexpression, increased MOR expression in parabrachial nucleus, and increased c-Fos expression in rostral ventromedial medulla. ULF-TENS may represent a novel and applicable therapeutic approach for improvement of orofacial pain induced by MMP.
A Novel KCNA2 Variant in a Patient with Non-Progressive Congenital Ataxia and Epilepsy: Functional Characterization and Sensitivity to 4-Aminopyridine
Authors: Imbrici Paola , Conte Elena , Blunck Rikard , Stregapede Fabrizia , Liantonio Antonella , Tosi Michele , D’Adamo Maria Cristina , De Luca Annamaria , Brankovic Vesna , Zanni Ginevra
Published: September 2021

Kv1.2 channels, encoded by the KCNA2 gene, are localized in the central and peripheral nervous system, where they regulate neuronal excitability. Recently, heterozygous mutations in KCNA2 have been associated with a spectrum of symptoms extending from epileptic encephalopathy, intellectual disability, and cerebellar ataxia. Patients are treated with a combination of antiepileptic drugs and 4-aminopyridine (4-AP) has been recently trialed in specific cases. We identified a novel variant in KCNA2, E236K, in a Serbian proband with non-progressive congenital ataxia and early onset epilepsy, treated with sodium valproate. To ascertain the pathogenicity of E236K mutation and to verify its sensitivity to 4-AP, we transfected HEK 293 cells with Kv1.2 WT or E236K cDNAs and recorded potassium currents through the whole-cell patch-clamp. In silico analysis supported the electrophysiological data. E236K channels showed voltage-dependent activation shifted towards negative potentials and slower kinetics of deactivation and activation compared with Kv1.2 WT. Heteromeric Kv1.2 WT+E236K channels, resembling the condition of the heterozygous patient, confirmed a mixed gain- and loss-of-function (GoF/LoF) biophysical phenotype. 4-AP inhibited both Kv1.2 and E236K channels with similar potency. Homology modeling studies of mutant channels suggested a reduced interaction between the residue K236 in the S2 segment and the gating charges at S4. Overall, the biophysical phenotype of E236K channels correlates with the mild end of the clinical spectrum reported in patients with GoF/LoF defects. The response to 4-AP corroborates existing evidence that KCNA2-disorders could benefit from variant-tailored therapeutic approaches, based on functional studies.
The Human Microbiomes in Pancreatic Cancer: Towards Evidence-Based Manipulation Strategies?
Authors: Brandi Giovanni , Turroni Silvia , McAllister Florencia , Frega Giorgio
Published: September 2021

Recent pieces of evidence have emerged on the relevance of microorganisms in modulating responses to anticancer treatments and reshaping the tumor-immune microenvironment. On the one hand, many studies have addressed the role of the gut microbiota, providing interesting correlative findings with respect to etiopathogenesis and treatment responses. On the other hand, intra-tumoral bacteria are being recognized as intrinsic and essential components of the cancer microenvironment, able to promote a plethora of tumor-related aspects from cancer growth to resistance to chemotherapy. These elements will be probably more and more valuable in the coming years in early diagnosis and risk stratification. Furthermore, microbial-targeted intervention strategies may be used as adjuvants to current therapies to improve therapeutic responses and overall survival. This review focuses on new insights and therapeutic approaches that are dawning against pancreatic cancer: a neoplasm that arises in a central metabolic “hub” interfaced between the gut and the host.
Platelet Inhibition by Low-Dose Acetylsalicylic Acid Reduces Neuroinflammation in an Animal Model of Multiple Sclerosis
Authors: Vogelsang Anna , Eichler Susann , Huntemann Niklas , Masanneck Lars , Böhnlein Hannes , Schüngel Lisa , Willison Alice , Loser Karin , Nieswandt Bernhard , Kehrel Beate E. , Zarbock Alexander , Göbel Kerstin , Meuth Sven G.
Published: September 2021

Aside from the established immune-mediated etiology of multiple sclerosis (MS), compelling evidence implicates platelets as important players in disease pathogenesis. Specifically, numerous studies have highlighted that activated platelets promote the central nervous system (CNS)-directed adaptive immune response early in the disease course. Platelets, therefore, present a novel opportunity for modulating the neuroinflammatory process that characterizes MS. We hypothesized that the well-known antiplatelet agent acetylsalicylic acid (ASA) could inhibit neuroinflammation by affecting platelets if applied at low-dose and investigated its effect during experimental autoimmune encephalomyelitis (EAE) as a model to study MS. We found that oral administration of low-dose ASA alleviates symptoms of EAE accompanied by reduced inflammatory infiltrates and less extensive demyelination. Remarkably, the percentage of CNS-infiltrated CD4+ T cells, the major drivers of neuroinflammation, was decreased to 40.98 ± 3.28% in ASA-treated mice compared to 56.11 ± 1.46% in control animals at the disease maximum as revealed by flow cytometry. More interestingly, plasma levels of thromboxane A2 were decreased, while concentrations of platelet factor 4 and glycoprotein VI were not affected by low-dose ASA treatment. Overall, we demonstrate that low-dose ASA could ameliorate the platelet-dependent neuroinflammatory response in vivo, thus indicating a potential treatment approach for MS.
Role of Iron-Containing Alcohol Dehydrogenases in Acinetobacter baumannii ATCC 19606 Stress Resistance and Virulence
Authors: Lin Guang-Huey , Hsieh Ming-Chuan , Shu Hung-Yu
Published: September 2021

Most bacteria possess alcohol dehydrogenase (ADH) genes (Adh genes) to mitigate alcohol toxicity, but these genes have functions beyond alcohol degradation. Previous research has shown that ADH can modulate quorum sensing in Acinetobacter baumannii, a rising opportunistic pathogen. However, the number and nature of Adh genes in A. baumannii have not yet been fully characterized. We identified seven alcohol dehydrogenases (NAD+-ADHs) from A. baumannii ATCC 19606, and examined the roles of three iron-containing ADHs, ADH3, ADH4, and ADH6. Marker-less mutation was used to generate Adh3, Adh4, and Adh6 single, double, and triple mutants. Disrupted Adh4 mutants failed to grow in ethanol-, 1-butanol-, or 1-propanol-containing mediums, and recombinant ADH4 exhibited strongest activity against ethanol. Stress resistance assays with inorganic and organic hydroperoxides showed that Adh3 and Adh6 were key to oxidative stress resistance. Virulence assays performed on the Galleria mellonella model organism revealed that Adh4 mutants had comparable virulence to wild-type, while Adh3 and Adh6 mutants had reduced virulence. The results suggest that ADH4 is primarily involved in alcohol metabolism, while ADH3 and ADH6 are key to stress resistance and virulence. Further investigation into the roles of other ADHs in A. baumannii is warranted.
Cyclic GMP-Dependent Regulation of Vascular Tone and Blood Pressure Involves Cysteine-Rich LIM-Only Protein 4 (CRP4)
Authors: Längst Natalie , Adler Julia , Schweigert Olga , Kleusberg Felicia , Cruz Santos Melanie , Knauer Amelie , Sausbier Matthias , Zeller Tanja , Ruth Peter , Lukowski Robert
Published: September 2021

The cysteine-rich LIM-only protein 4 (CRP4), a LIM-domain and zinc finger containing adapter protein, has been implicated as a downstream effector of the second messenger 3′,5′-cyclic guanosine monophosphate (cGMP) pathway in multiple cell types, including vascular smooth muscle cells (VSMCs). VSMCs and nitric oxide (NO)-induced cGMP signaling through cGMP-dependent protein kinase type I (cGKI) play fundamental roles in the physiological regulation of vascular tone and arterial blood pressure (BP). However, it remains unclear whether the vasorelaxant actions attributed to the NO/cGMP axis require CRP4. This study uses mice with a targeted deletion of the CRP4 gene (CRP4 KO) to elucidate whether cGMP-elevating agents, which are well known for their vasorelaxant properties, affect vessel tone, and thus, BP through CRP4. Cinaciguat, a NO- and heme-independent activator of the NO-sensitive (soluble) guanylyl cyclase (NO-GC) and NO-releasing agents, relaxed both CRP4-proficient and -deficient aortic ring segments pre-contracted with prostaglandin F2α. However, the magnitude of relaxation was slightly, but significantly, increased in vessels lacking CRP4. Accordingly, CRP4 KO mice presented with hypotonia at baseline, as well as a greater drop in systolic BP in response to the acute administration of cinaciguat, sodium nitroprusside, and carbachol. Mechanistically, loss of CRP4 in VSMCs reduced the Ca2+-sensitivity of the contractile apparatus, possibly involving regulatory proteins, such as myosin phosphatase targeting subunit 1 (MYPT1) and the regulatory light chain of myosin (RLC). In conclusion, the present findings confirm that the adapter protein CRP4 interacts with the NO-GC/cGMP/cGKI pathway in the vasculature. CRP4 seems to be part of a negative feedback loop that eventually fine-tunes the NO-GC/cGMP axis in VSMCs to increase myofilament Ca2+ desensitization and thereby the maximal vasorelaxant effects attained by (selected) cGMP-elevating agents.
Human Hazard Assessment Using Drosophila Wing Spot Test as an Alternative In Vivo Model for Genotoxicity Testing—A Review
Authors: Pitchakarn Pornsiri , Inthachat Woorawee , Karinchai Jirarat , Temviriyanukul Piya
Published: September 2021

Genomic instability, one of cancer’s hallmarks, is induced by genotoxins from endogenous and exogenous sources, including reactive oxygen species (ROS), diet, and environmental pollutants. A sensitive in vivo genotoxicity test is required for the identification of human hazards to reduce the potential health risk. The somatic mutation and recombination test (SMART) or wing spot test is a genotoxicity assay involving Drosophila melanogaster (fruit fly) as a classical, alternative human model. This review describes the principle of the SMART assay in conjunction with its advantages and disadvantages and discusses applications of the assay covering all segments of health-related industries, including food, dietary supplements, drug industries, pesticides, and herbicides, as well as nanoparticles. Chemopreventive strategies are outlined as a global health trend for the anti-genotoxicity of interesting herbal extract compounds determined by SMART assay. The successful application of Drosophila for high-throughput screening of mutagens is also discussed as a future perspective.
Generation and Characterization of a New FRET-Based Ca2+ Sensor Targeted to the Nucleus
Authors: Galla Luisa , Vajente Nicola , Pendin Diana , Pizzo Paola , Pozzan Tullio , Greotti Elisa
Published: September 2021

Calcium (Ca2+) exerts a pivotal role in controlling both physiological and detrimental cellular processes. This versatility is due to the existence of a cell-specific molecular Ca2+ toolkit and its fine subcellular compartmentalization. Study of the role of Ca2+ in cellular physiopathology greatly benefits from tools capable of quantitatively measuring its dynamic concentration ([Ca2+]) simultaneously within organelles and in the cytosol to correlate localized and global [Ca2+] changes. To this aim, as nucleoplasm Ca2+ changes mirror those of the cytosol, we generated a novel nuclear-targeted version of a Föster resonance energy transfer (FRET)-based Ca2+ probe. In particular, we modified the previously described nuclear Ca2+ sensor, H2BD3cpv, by substituting the donor ECFP with mCerulean3, a brighter and more photostable fluorescent protein. The thorough characterization of this sensor in HeLa cells demonstrated that it significantly improved the brightness and photostability compared to the original probe, thus obtaining a probe suitable for more accurate quantitative Ca2+ measurements. The affinity for Ca2+ was determined in situ. Finally, we successfully applied the new probe to confirm that cytoplasmic and nucleoplasmic Ca2+ levels were similar in both resting conditions and upon cell stimulation. Examples of simultaneous monitoring of Ca2+ signal dynamics in different subcellular compartments in the very same cells are also presented.
Deep Sequencing Discovery and Profiling of Known and Novel miRNAs Produced in Response to DNA Damage in Rice
Authors: Zhang Jianxiang , Xu Cheng , Liu Kangwei , Li Yaoqinq , Wang Mengna , Tao Lian , Yu Hengxiu , Zhang Chao
Published: September 2021

Under extreme environmental conditions such as ultraviolet and ionizing radiation, plants may suffer DNA damage. If these damages are not repaired accurately and rapidly, they may lead to chromosomal abnormalities or even cell death. Therefore, organisms have evolved various DNA repair mechanisms to cope with DNA damage which include gene transcription and post-translational regulation. MicroRNA (miRNA) is a type of non-coding single-stranded RNA molecule encoded by endogenous genes. They can promote DNA damage repair by regulating target gene transcription. Here, roots from seedlings of the japonica rice cultivar ‘Yandao 8’ that were treated with bleomycin were collected for transcriptome-level sequencing, using non-treated roots as controls. A total of 14,716,232 and 17,369,981 reads mapping to miRNAs were identified in bleomycin-treated and control groups, respectively, including 513 known and 72 novel miRNAs. Compared with the control group, 150 miRNAs showed differential expression levels. Target predictions of these differentially expressed miRNAs yielded 8731 potential gene targets. KEGG annotation and a gene ontology analysis indicated that the highest-ranked target genes were classified into metabolic processes, RNA degradation, DNA repair, and so on. Notably, the DNA repair process was significantly enriched in both analyses. Among these differentially expressed miRNAs, 58 miRNAs and 41 corresponding potential target genes were predicted to be related to DNA repair. RT-qPCR results confirmed that the expression patterns of 20 selected miRNAs were similar to those from the sequencing results, whereas four miRNAs gave opposite results. The opposing expression patterns of several miRNAs with regards to their target genes relating to the DNA repair process were also validated by RT-qPCR. These findings provide valuable information for further functional studies of miRNA involvement in DNA damage repair in rice.