bluuebunny/crossref_metadata_2025 huggingface dataset:
Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas
Authors: Justiz-Vaillant Angel , Gopaul Darren , Soodeen Sachin , Unakal Chandrashekhar , Thompson Reinand , Pooransingh Shalini , Arozarena-Fundora Rodolfo , Asin-Milan Odalis , Akpaka Patrick Eberechi
Published: August 2024

Immunology and microbiology research has witnessed remarkable growth and innovation globally, playing a pivotal role in advancing our understanding of immune mechanisms, disease pathogenesis, and therapeutic interventions. This manuscript presents a comprehensive exploration of the key areas in immunology research, spanning from the utilisation of bacterial proteins as antibody reagents to the intricate realms of clinical immunology and disease management. The utilisation of bacterial immunoglobulin-binding proteins (IBPs), including protein A (SpA), protein G (SpG), and protein L (SpL), has revolutionised serological diagnostics, showing promise in early disease detection and precision medicine. Microbiological studies have shed light on antimicrobial resistance patterns, particularly the emergence of extended-spectrum beta-lactamases (ESBLs), guiding antimicrobial stewardship programmes and informing therapeutic strategies. Clinical immunology research has elucidated the molecular pathways underlying immune-mediated disorders, resulting in tailored management strategies for conditions such as severe combined immunodeficiency (SCID), neuropsychiatric systemic lupus erythematosus (NPSLE), etc. Additionally, significant efforts in vaccine development against tuberculosis and HIV are highlighted, underscoring the ongoing global pursuit of effective preventive measures against these infectious diseases. In summary, immunology and microbiology research have provided significant contributions to global healthcare, fostering collaboration, innovation, and improved patient outcomes.
Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics
Authors: Gouvias Ioannis , Lysitsas Marios , Batsidis Apostolos , Malefaki Sonia , Bitchava Dimitra , Tsara Anna , Nickovic Emilija , Bouzalas Ilias , Malissiova Eleni , Guatteo Raphaël , Valiakos George
Published: August 2024

The objective of this study was to apply and preliminarily evaluate a High-Resolution Melting (HRM) analysis technique coupled with qPCR, that allows the simultaneous detection of 10 different ruminant abortogenic pathogens, for investigating abortions in sheep and goats throughout Greece. A total of 264 ovine and caprine vaginal swabs were obtained the week following the abortion from aborted females and analyzed using a commercially available kit (ID Gene™ Ruminant Abortion Multiplex HRM, Innovative Diagnostics). Results indicated a high prevalence of Coxiella burnetii and Chlamydophila spp., which were detected in 48.9% and 42.4% of the vaginal swabs, respectively. Results for these most commonly detected pathogens were compared with those of a well-established commercial qPCR kit, with near-perfect agreement. Toxoplasma gondii, Salmonella spp., Brucella spp., Anaplasma phagocytophilum, Campylobacter fetus, and Neospora caninum were also identified, the two latter reported for the first time in the country in small ruminants. Mixed infections occurred in 35.6% of the animals examined. This technique allows for the simultaneous detection of many abortogenic pathogens in an accurate and cost-effective assay. Detection of uncommon or not previously reported pathogens in various cases indicates that their role in ovine and caprine abortions may be underestimated.
Al-Si Order and Chemical Composition Model across Scapolite Solid Solutions with Evidence from Rietveld Structure Refinements
Authors: Antao Sytle M.
Published: August 2024

Scapolite forms solid solutions between the end members marialite, Na4[Al3Si9O24]Cl = Me0, and meionite, Ca4[Al6Si6O24]CO3 = Me100. Al-Si order and chemical composition models are proposed for the scapolite solid solutions. These models predict the chemical composition, Al-Si order, and average  distances between Me0–Me100. These models are based on the observed order of clusters and on two solid solutions that meet at Me75 coupled with predicted chemical compositions and  distances. The [Na4·Cl]3+ and [NaCa3·CO3]5+ clusters are ordered between Me0–Me75, whereas the clusters [NaCa3·CO3]5+ and [Ca4·CO3]6+ are disordered from Me75–Me100. To confirm the structural model, the crystal structure of 27 scapolite samples between Me6–Me93 has been obtained using synchrotron high-resolution powder X-ray diffraction (HRPXRD) data and Rietveld structure refinements. The structure was refined in space group P42/n for all the samples. The  distances indicate that the T1 (=Si), T2 (=Al), and T3 (=Si) sites are completely ordered at Me37.5, where the 1:1 ratio of [Na4·Cl]3+:[NaCa3·CO3]5+ clusters are ordered and gives rise to antiphase domain boundaries (APBs) based on Cl-CO3 order instead of Al-Si order. The presence of APBs based on Cl-CO3 order and cluster order indicate that neither space group P42/n nor I4/m are correct for the structure of scapolite, but the lower symmetry space group P42/n is a good approximation for modeling the average structure of scapolite. The complete Al-Si order at Me37.5 changes in a regular and predictable manner toward the end members: Me0, Me75, and Me100. The observed unit cell and several structural parameters show a discontinuity at Me75, where the series is divided into two. There is no structural evidence to support any phase transition in the scapolite series. The T1 site contains only Si from Me0–Me37.5; from Me37.5–Me100, Al atoms enter the T1 site and the  distance increases linearly to Me100.
Study on Column Leaching Behavior of Low-Grade High Calcium and Magnesium Copper Ore
Authors: Xu Jingyuan , Qin Shuchen , Zheng Chaozhen , Sun Honghong , Yang Baojun , Liu Sanping , Qiu Guanzhou , Northwood Derek O. , Waters Kristian E. , Ma Hao
Published: August 2024

This paper studies the process mineralogy, mechanism, and kinetics of column leaching behavior of low-grade high-calcium–magnesium copper ore. The effect of sulfuric acid concentration, leach solution spraying intensity, and material particle size on column leaching kinetics is discussed. The kinetic analysis of column leaching of copper indicates that sulfuric acid concentration has a significant impact. As sulfuric acid concentration increases, the limiting step of reaction shifts from chemical reaction control to a combination of chemical reaction and diffusion mixing control. Spraying intensity also affects copper column leaching; increasing intensity shifts the limiting step from diffusion control to mixing control, thereby mitigating the effects of diffusion control. Regarding other elements, it is found that iron leaching is primarily controlled by chemical reaction, while calcium leaching is mainly controlled by chemical reaction. As sulfuric acid concentration increases from 10 g/L to 20 g/L, the limiting step for calcium leaching shifts from chemical reaction control to chemical reaction and diffusion-mixing control.
A Systems Engineering Approach to Incorporate ESG Risks and Opportunities in Early-Stage Mine Design and Planning
Authors: Nehring Micah , Knights Peter
Published: August 2024

This study examines how ESG risks and opportunities can be systematically identified, assessed, and incorporated into the early-stage design and planning of natural resources projects. The focus for this study will be on the mining activities required to source the resources for the global decarbonization effort. The need for a framework to incorporate ESG risks and opportunities into the strategic mine planning process was first identified in the de Beers Sustainability Valuation Approach. The Social Value Capital Decision Model advanced by BHP represents an advance on the de Beers model. This is the first example of a structured methodology for systematically considering stakeholder values and incorporating these into the capital decision framework. To test the applicability of a new approach to mine design by using Quality Function Deployment (QFD), a case study involving a copper mine located in South America was developed. This case study demonstrates how QFD can provide clear line-of-sight to connect design decisions with priority stakeholder concerns. The framework provides a communications tool for aligning the ESG design process across functional silos within complex organizations. The development of appropriate software tools could assist in managing the inherent complexity associated with integrating stakeholder value concerns into early stage design decisions.
Novel Quinazoline Derivatives as Highly Effective A2A Adenosine Receptor Antagonists
Authors: Laversin Amélie , Dufossez Robin , Bolteau Raphaël , Duroux Romain , Ravez Séverine , Hernandez-Tapia Sergio , Fossart Martin , Coevoet Mathilde , Liberelle Maxime , Yous Saïd , Lebègue Nicolas , Melnyk Patricia
Published: August 2024

The adenosine A2A receptor (A2AR) has been identified as a therapeutic target for treating neurodegenerative diseases and cancer. In recent years, we have highlighted the 2-aminoquinazoline heterocycle as an promising scaffold for designing new A2AR antagonists, exemplified by 6-bromo-4-(furan-2-yl)quinazolin-2-amine 1 (Ki (hA2AR) = 20 nM). Here, we report the synthesis of new 2-aminoquinazoline derivatives with substitutions at the C6- and C7-positions, and the introduction of aminoalkyl chains containing tertiary amines at the C2-position to enhance antagonist activity and solubility properties. Compound 5m showed a high affinity for hA2AR with a Ki value of 5 nM and demonstrated antagonist activity with an IC50 of 6 µM in a cyclic AMP assay. Introducing aminopentylpiperidine and 4-[(piperidin-1-yl)methyl]aniline substituents maintained the binding affinities (9x, Ki = 21 nM; 10d, Ki = 15 nM) and functional antagonist activities (9x, IC50 = 9 µM; 10d, IC50 = 5 µM) of the synthesized compounds while improving solubility. This study provides insights into the future development of A2AR antagonists for therapeutic applications.
Aqueous Phase Hydrogenation of 4-(2-Furyl)-3-buten-2-one over Different Re Phases
Authors: Díaz Claudio Ignacio C. , Araya-López Claudio , Dongil A. B. , Escalona Nestor
Published: August 2024

4-(2-furyl)-3-buten-2-one (FAc) is obtained by aldol condensation of furfural and acetone and has been used in hydrodeoxygenation reactions to obtain fuel products using noble metal catalysts. The hydrogenation of FAc in the aqueous phase using metallic- and Re oxide-supported catalysts on graphite was studied, within a temperature range of 200–240 °C, in a batch reactor over a 6 h reaction period. The catalysts were characterized using N2 adsorption–desorption, TPR-H2, TPD-NH3, XRD, and XPS analyses. Catalytic reactions revealed that metallic rhenium and rhenium oxide-supported catalysts are active for the hydrogenation and Piancatelli rearrangement of FAc. Notably, metallic rhenium exhibited a fourfold higher initial rate than rhenium oxide, which was attributed to the higher dispersion of Re in the Re/G catalyst over graphite. Re/G and ReOx/G catalysts tended to rearrange and hydrogenate FAc to 2-(2-oxopropyl)cyclopenta-1-one in water.
Synthesis of High-Purity Hydroxyapatite and Phosphoric Acid Derived from Moroccan Natural Phosphate Rocks by Minimizing Cation Content Using Dissolution–Precipitation Technique
Authors: Benataya Karim , Lakrat Mohammed , Hammani Othmane , Aaddouz Mohamed , Ait Yassine Youssef , Abuelizz Hatem A. , Zarrouk Abdelkader , Karrouchi Khalid , Mejdoubi Elmiloud
Published: August 2024

This study investigates, in the first part, the synthesis and purification of a poorly crystalline hydroxyapatite (HAp) using natural Moroccan phosphate (Boucraa region) as a raw material. Despite its successful preparation, the obtained HAp was contaminated by several metallic cations (mostly Cd, Pb, Sn, Ti, Mn, Mg, Fe, and Al) migrated from the natural rocks during the digestion process, inhibiting HAp application in several sectors. To minimize the existence of these elements, the dissolution–precipitation technique (DP) was investigated as a non-selective purification process. Following the initial DP cycle conducted on the precipitated HAp, the removal efficiency was approximately 60% for Al, Fe, Mg, Mn, and Ti and 90% for Cd and Pb. After three consecutive DP cycles, notable improvement in the removal efficiency was observed, reaching 66% for Fe, 69% for Mg, 73% for Mn, and 74% for Al, while Cd, Pb, and Ti were totally removed. In the second part of this study, the purified HAp was digested using sulfuric acid to produce high-quality phosphoric acid (PA) and gypsum (GP). The elemental analysis of the PA indicates a removal efficiency of approximately 89% for Fe and over 94% for all the examined cations. In addition, the generated GP was dominated by SO3 and CaO accompanied with minor impurities. Overall, this simple process proves to be practically useful, to reduce a broad spectrum of cationic impurities, and to be flexible to prepare valuable products such hydroxyapatite, phosphoric acid, and gypsum.
The Utilization of Rice Husk as Both the Silicon Source and Mesoporous Template for the Green Preparation of Mesoporous TiO2/SiO2 and Its Excellent Catalytic Performance in Oxidative Desulfurization
Authors: Liu Xiaoxue , Zhang Lanfen , Hu Jian , Zhang Wei , Xiang Xiaorong , Cheng Huiqing , Qin Li , Li Hao
Published: August 2024

In recent years, TiO2-based catalysts have received extensive attention from researchers for their excellent oxidative desulfurization (ODS) performances. In this paper, a series of mesoporous TiO2/SiO2 catalysts with different TiO2 loadings are prepared, using an incipient wetness impregnation method with agricultural waste rice husk as both the silicon source and mesoporous template and tetrabutyl titanate as the titanium source. The effect of different TiO2 loadings on the ODS performance of the samples is investigated, and the appropriate TiO2 loading is 2.5%. Compared with pure TiO2, the 2.5%TiO2/SiO2 sample exhibits high catalytic activity for oxidative desulfurization. This is, on the one hand, due to the high specific surface area and mesopore volume of the 2.5%TiO2/SiO2 sample. On the other hand, it is due to the uniform dispersion of TiO2 grains with an average diameter of 6.1 nm on the surface of the mesoporous SiO2 carrier, which greatly increases the active sites of the 2.5%TiO2/SiO2 sample, thus improving the catalytic activity of the sample. The recycling performances of the 2.5%TiO2/SiO2 sample are further investigated. The results show that, after fifteen cycles, the 2.5%TiO2/SiO2 sample still maintains high conversions of dibenzothiophene (99.8%) and 4,6-dimethyldibenzothiophene (99.7%) without deactivation. In addition, the 2.5%TiO2/SiO2 sample treated with TBHP aqueous solution is characterized by the technique of UV-Vis, and the Ti-peroxo (Ti-OOtBu) species, the active intermediate for the ODS of bulky organic sulfides, is successfully captured. Finally, a possible reaction mechanism for the ODS process over the 2.5%TiO2/SiO2 sample is proposed.
Optical Fiber Probe with Integrated Micro-Optical Filter for Raman and Surface-Enhanced Raman Scattering Sensing
Authors: Al Mamun Md Abdullah , Katkus Tomas , Mahadevan-Jansen Anita , Juodkazis Saulius , Stoddart Paul R.
Published: August 2024

Optical fiber Raman and surface-enhanced Raman scattering (SERS) probes hold great promise for in vivo biosensing and in situ monitoring of hostile environments. However, the silica Raman scattering background generated within the optical fiber increases in proportion to the length of the fiber, and it can swamp the signal from the target analyte. While filtering can be applied at the distal end of the fiber, the use of bulk optical elements has limited probe miniaturization to a diameter of 600 µm, which in turn limits the potential applications. To overcome this limitation, femtosecond laser micromachining was used to fabricate a prototype micro-optical filter, which was directly integrated on the tip of a 125 µm diameter double-clad fiber (DCF) probe. The outer surface of the microfilter was further modified with a nanostructured, SERS-active, plasmonic film that was used to demonstrate proof-of-concept performance with thiophenol as a test analyte. With further optimization of the associated spectroscopic system, this ultra-compact microprobe shows great promise for Raman and SERS optical fiber sensing.
Hybrid Cellulosic Substrates Impregnated with Meta-PBI-Stabilized Carbon Nanotubes/Plant Extract-Synthesized Zinc Oxide—Antibacterial and Photocatalytic Dye Degradation Study
Authors: Penchev Hristo , Zaharieva Katerina , Dimova Silvia , Grancharov Georgy , Petrov Petar D. , Shipochka Maria , Dimitrov Ognian , Lazarkevich Irina , Engibarov Stephan , Eneva Rumyana
Published: August 2024

Novel fibrous cellulosic substrates impregnated with meta-polybenzimidazole (PBI)-stabilized carbon nanotubes/zinc oxide with different weight content of ZnO and with the use of dimethylacetamide as dispersant media. The pristine ZnO nanoparticle powder was prepared by plant extract-mediated synthesis using Vaccinium vitis-idaea L. The green synthesized ZnO possesses an average crystallite size of 15 nm. The formation of agglomerates from ZnO NPs with size 250 nm–350 nm in the m-PBI@CNTs/ZnO was determined. The prepared materials were investigated by PXRD analysis, XPS, SEM, EDS, AFM, and TEM in order to establish the phase and surface composition, structure, and morphology of the hybrids. The potential of the synthesized hybrid composites to degrade methylene blue (MB) dye as a model contaminant in aqueous solutions under UV illumination was studied. The photocatalytic results show that in the course of the photocatalytic reaction, the m-PBI@CNTs/ZnO 1:3 photocatalyst leads to the highest degree of degradation of the methylene blue dye (67%) in comparison with the other two studied m-PBI@CNTs/ZnO 1:1 and 1:2 composites (48% and 41%). The antibacterial activity of ZnO nanoparticles and the hybrid CNT materials was evaluated by the RMDA and the dynamic contact method, respectively. The profound antibacterial effect of the m-PBI@CNTs/ZnO hybrids was monitored for 120 h of exposition in dark and UV illumination regimes. The photocatalytic property of ZnO nanoparticles significantly shortens the time for bactericidal action of the composites in both regimes. The m-PBI@CNTs/ZnO 1:2 combination achieved complete elimination of 5.105 CFU/mL E. coli cells after 10 min of UV irradiation.
Therapeutic Potential of Various Intermittent Fasting Regimens in Alleviating Type 2 Diabetes Mellitus and Prediabetes: A Narrative Review
Authors: Msane Sthembiso , Khathi Andile , Sosibo Aubrey
Published: August 2024

Intermittent fasting has drawn significant interest in the clinical research community due to its potential to address metabolic complications such as obesity and type 2 diabetes mellitus. Various intermittent fasting regimens include alternate-day fasting (24 h of fasting followed by 24 h of eating), time-restricted fasting (fasting for 14 h and eating within a 10 h window), and the 5:2 diet (fasting for two days and eating normally for the other five days). Intermittent fasting is associated with a reduced risk of type 2 diabetes mellitus-related complications and can slow their progression. The increasing global prevalence of type 2 diabetes mellitus highlights the importance of early management. Since prediabetes is a precursor to type 2 diabetes mellitus, understanding its progression is essential. However, the long-term effects of intermittent fasting on prediabetes are not yet well understood. Therefore, this review aims to comprehensively compile existing knowledge on the therapeutic effects of intermittent fasting in managing type 2 diabetes mellitus and prediabetes.
Nutritional Quality of Breakfast Cereals on the French, Belgian and Luxembourg Markets: Which Cereals for Children?
Authors: Robert Martine , Martin Françoise , Xhonneux Annick , Mosser Françoise , Favre Elisabeth , Richonnet Celine
Published: August 2024

Objective: Analyse the breakfast cereal market to help to help healthcare professionals to guide parents in choosing healthy products for their children. Study design: Observational study of the breakfast cereals available in the biggest supermarkets, discount stores and organic chains in France, Belgium and Luxembourg. Methods: An analysis of nutritional qualities using three indicators: Nutri-Score (initial and modified version), WHO Europe nutrient profile model, and Nova. Results: 645 products were listed; 559 excluding duplicates. A total of 28.8% are marketed to children and make up the group of “children’s” cereals, 62.1% of cereals are Muesli, Oats and other cereal flakes (MOCF), and 54.9% are “organic”. The study shows that “children’s” cereals have a poorer nutritional profile: a higher proportion of Nutri-Score D, higher sugar content, lower fibre content, less conformity with the WHO Europe nutrient profile model and a higher proportion ofultra-processed. On the other hand, MOCF and “organic” products generally have a better nutritional profile: less sugar, more fibre, more Nutri-Score A, less Nutri-Score D and fewer ultra-processed products. Conclusions: Parents should therefore opt for cereals that do not bear any reference to children on the packaging.
Investigating a New Way to Assess Metabolic Risk in Pregnant Females with Prior RYGB Surgery
Authors: Gisinger Teresa , Reiter Birgit , Preindl Karin , Stimpfl Thomas , Gard Liliana-Imi , Baumgartner-Parzer Sabina , Kautzky-Willer Alexandra , Leutner Michael
Published: August 2024

Background: Obesity in pregnancy is linked to adverse clinical outcomes such as gestational diabetes. Recently, a risk score calculated by different ceramide concentrations was recognized as a new way to investigate cardiovascular risk. The aim was to analyze if the ceramide risk score and cardiometabolic risk vary between normal-weight, obese, and females with prior Roux-en-Y bypass surgery (RYGB) during pregnancy. Methods: Three cohorts were investigated: first, 25 pregnant females with a history of RYGB; second, 19 with preconception BMI ≥ 35 kg/m2; and third, 19 normal-weight (preconception BMI < 25 kg/m2). Around the 24th to 28th weeks of gestation routine laboratory assessments, 3 h 75 g oral and intravenous glucose tolerance tests were carried out. The correlation of ceramide risk scores and ceramide ratios (Cer(d18:1/18:0)/Cer(d18:1/16:0)) with metabolic parameters was analyzed via Pearson correlation. The cohorts were compared via ANOVA and unpaired t-tests. Results: The RYGB cohort had lower ceramide risk scores and ratios compared to obese pregnant females (7.42 vs. 9.34, p = 0.025; 0.33 vs. 0.47, p < 0.001). Ceramide risk score and ratio were found to correlate negatively with insulin sensitivity (measured with the Matsuda (r = −0.376, p = 0.031; r = −0.455, p = 0.008) and calculated sensitivity index (r = −0.358, p = 0.044; r = −0.621, p < 0.001) in females without RYGB. The ceramide risk score correlated positively with body fat in RYGB females (r = 0.650, p = 0.012). Conclusions: We found that females after RYGB have lower ceramide risk scores and ceramide ratios compared to obese pregnant females, possibly indicating lower metabolic risk.
CPT Symmetry Searches in the Neutral Meson System
Authors: Roberts Ágnes
Published: August 2024

A review of the landscape of CPT symmetry tests is presented, centered around the Standard-Model Extension and focusing on tests in the neutral meson system. A discussion of the relevant theories summarizes original ideas. It is followed by a short transition into phenomenology. A more detailed parameterization is presented. Various experiments are used to deliver an overview of testing CPT from every angle that the theory suggested and that the neutral meson (NM) system could accommodate.
Identifying the Drivers Related to Animal Reservoirs, Environment, and Socio-Demography of Human Leptospirosis in Different Community Types of Southern Chile: An Application of Machine Learning Algorithm in One Health Perspective
Authors: Talukder Himel , Muñoz-Zanzi Claudia , Salgado Miguel , Berg Sergey , Yang Anni
Published: August 2024

Leptospirosis is a zoonosis with global public health impact, particularly in poor socio-economic settings in tropical regions. Transmitted through urine-contaminated water or soil from rodents, dogs, and livestock, leptospirosis causes over a million clinical cases annually. Risk factors include outdoor activities, livestock production, and substandard housing that foster high densities of animal reservoirs. This One Health study in southern Chile examined Leptospira serological evidence of exposure in people from urban slums, semi-rural settings, and farm settings, using the Extreme Gradient Boosting algorithm to identify key influencing factors. In urban slums, age, shrub terrain, distance to Leptospira-positive households, and neighborhood housing density were contributing factors. Human exposure in semi-rural communities was linked to environmental factors (trees, shrubs, and lower vegetation terrain) and animal variables (Leptospira-positive dogs and rodents and proximity to Leptospira-positive households). On farms, dog counts, animal Leptospira prevalence, and proximity to Leptospira-contaminated water samples were significant drivers. The study underscores that disease dynamics vary across landscapes, with distinct drivers in each community setting. This case study demonstrates how the integration of machine learning with comprehensive cross-sectional epidemiological and geospatial data provides valuable insights into leptospirosis eco-epidemiology. These insights are crucial for informing targeted public health strategies and generating hypotheses for future research.
Polyene-Based Derivatives with Antifungal Activities
Authors: Ngece Kwanele , Ntondini Thabisa L. , Khwaza Vuyolwethu , Paca Athandwe M. , Aderibigbe Blessing A.
Published: August 2024

Polyenes are a class of organic compounds well known for their potent antifungal properties. They are effective due to their ability to target and disrupt fungal cell membranes by binding to ergosterol and forming pores. Despite their effectiveness as antifungal drugs, polyenes have several limitations, such as high toxicity to the host cell and poor solubility in water. This has prompted ongoing research to develop safer and more efficient derivatives to overcome such limitations while enhancing their antifungal activity. In this review article, we present a thorough analysis of polyene derivatives, their structural modifications, and their influence on their therapeutic effects against various fungal strains. Key studies are discussed, illustrating how structural modifications have led to improved antifungal properties. By evaluating the latest advancements in the synthesis of polyene derivatives, we highlight that incorporating amide linkers at the carboxylic moiety of polyene molecules notably improves their antifungal properties, as evidenced by derivatives 4, 5, 6G, and 18. This review can help in the design and development of novel polyene-based compounds with potent antifungal activities.
Biorefinery of Beach Cast Seaweed in Brazil: Renewable Energy and Sustainability
Authors: Coelho Fernando Pinto , Menezes Rômulo Simões C. , Sampaio Everardo Valadares de S. B. , Barboza Márcio Gomes , Soares Emerson Carlos , Guedes-Coelho Elica Amara C. , França Elvis J. de , Santos Agnaldo J. dos , Lima Marcelo F. de , Costa Manoel Messias da S. , Ferrão Natache Gonçalves de M. , Soares Bruno M. , Nascimento Diego M. do , Carneiro Victor Andrei R. , Abreu Cesar Augusto M. de
Published: August 2024

Macroalgae are a natural oceanic resource of inexhaustible abundance for the biomass energy industry with growth rates that are three to four times greater than those of terrestrial plants. The objective of this study was to evaluate the sustainability of macroalgae as biomass for biorefining through two investigations. Firstly, the deposition of macroalgae was sampled through 28 collections on seven beaches in the city of Maceió, Brazil, over a two-year period using a zigzag sampling method, covering a deposition area of 135,000 m2. From this, it was estimated that daily collection would yield 5.03 t/ha of dry biomass. Secondly, the calorific values of macroalgal biomass energy and pellet compounds were calculated. The lower calorific value (8.82 MJ/kg) found from a compound of 13 species analyzed was similar to that of the main biomass used in Brazil to obtain energy, i.e., sugarcane bagasse, which has been evaluated as 8.91 MJ/kg. Macroalgal biomass in the form of condensed energy pellets was found to have a higher calorific value of 20.18 MJ/kg, i.e., 1.2% greater than the average for terrestrial biomass pellets. Based on the results obtained, it was observed that macroalgal biomass has the possibility of becoming a new renewable feedstock with potential for bioenergy. The estimates for the deposition of biomass show possibilities for producing biofuels from marine algal raw material, which provides scope for creating another sustainable alternative for global energy issues with a reduction in environmental problems.
Asphalt-Binder Mixtures Evaluated by T1 NMR Relaxometry
Authors: Herndon Rebecca M. , Balasubramanian Jay , Abdelrahman Magdy , Woelk Klaus
Published: August 2024

Asphalt pavements make up a majority of the essential transportation systems in the US. Asphalt mixtures age and degrade over time, reducing the pavement performance. Pavement performance critically depends on the aging of asphalt binder. The aging of asphalt binder during construction is traditionally modeled by rolling thin film oven (RTFO) testing, while aging during service life is modeled by pressure aging vessel (PAV) testing. Comparing these models to the aging of binders in actual pavements is limited because, to be used for current testing, binders must be separated from the pavement’s aggregate by solvent extraction. Solvent extraction will, at least in part, compromise the structural integrity of asphalt binder samples. Spin-lattice NMR relaxometry has been shown to nondestructively evaluate asphalt properties in situ through the analysis of hydrogen environments. The molecular mobility of hydrogen environments and with it the stiffness of asphalt binder samples can be determined by characteristic T1 relaxation times, indicating the complexity of asphalt-binder aging. In this study, two laboratory-generated asphalt mixtures, a failed field sample, and several laboratory-aged binder samples are compared by NMR relaxometry. NMR relaxometry was found to be able to differentiate between asphalt samples based on their binder percentage. According to the relaxometry findings, the RTFO binder aging compared favorably to the 6% laboratory-mixed sample. The PAV aging, however, did not compare well to the relaxometry results found for the field-aged sample. The amount of aggregate was found to have an influence on the relaxation times of the binder in the mixed samples and an inverse proportionality of the binder content to the primary NMR relaxation time was detected. It is concluded that molecular water present in the pores of the aggregate material gives rise to such a relationship. The findings of this study lay the foundation for nondestructive asphalt performance evaluation by NMR relaxometry.
Nanosized-Selenium-Application-Mediated Cadmium Toxicity in Aromatic Rice at Different Stages
Authors: Cui Baoling , Luo Haowen , Yao Xiangbin , Xing Pipeng , Deng Sicheng , Zhang Qianqian , Yi Wentao , Gu Qichang , Peng Ligong , Yu Xianghai , Zuo Changjian , Wang Jingjing , Wang Yangbo , Tang Xiangru
Published: August 2024

Cadmium (Cd) pollution restricts the rice growth and poses a threat to human health. Nanosized selenium (NanoSe) is a new nano material. However, the effects of NanoSe application on aromatic rice performances under Cd pollution have not been reported. In this study, a pot experiment was conducted with two aromatic rice varieties and a soil Cd concentration of 30 mg/kg. Five NanoSe treatments were applied at distinct growth stages: (T1) at the initial panicle stage, (T2) at the heading stage, (T3) at the grain-filling stage, (T1+2) at both the panicle initial and heading stages, and (T1+3) at both the panicle initial and grain-filling stages. A control group (CK) was maintained without any application of Se. The results showed that, compared with CK, the T1+2 and T1+3 treatments significantly reduced the grain Cd content. All NanoSe treatments increased the grain Se content. The grain number per panicle, 1000-grain weight, and grain yield significantly increased due to NanoSe application under Cd pollution. The highest yield was recorded in T3 and T1+3 treatments. Compared with CK, all NanoSe treatments increased the grain 2-acetyl-1-pyrroline (2-AP) content and impacted the content of pyrroline-5-carboxylic acid and 1-pyrroline which are the precursors in 2-AP biosynthesis. In conclusion, the foliar application of NanoSe significantly reduced the Cd content, increased the Se content, and improved the grain yield and 2-AP content of aromatic rice. The best amendment was applying NanoSe at both the panicle initial and grain-filling stages.
Permanent Stress Adaptation and Unexpected High Light Tolerance in the Shade-Adapted Chlamydomonas priscui
Authors: Popson Devon , D’Silva Susanna , Wheeless Kaylie , Morgan-Kiss Rachael
Published: August 2024

The Antarctic photopsychrophile, Chlamydomonas priscui UWO241, is adapted to extreme environmental conditions, including permanent low temperatures, high salt, and shade. During long-term exposure to this extreme habitat, UWO241 appears to have lost several short-term mechanisms in favor of constitutive protection against environmental stress. This study investigated the physiological and growth responses of UWO241 to high-light conditions, evaluating the impacts of long-term acclimation to high light, low temperature, and high salinity on its ability to manage short-term photoinhibition. We found that UWO241 significantly increased its growth rate and photosynthetic activity at growth irradiances far exceeding native light conditions. Furthermore, UWO241 exhibited robust protection against short-term photoinhibition, particularly in photosystem I. Lastly, pre-acclimation to high light or low temperatures, but not high salinity, enhanced photoinhibition tolerance. These findings extend our understanding of stress tolerance in extremophilic algae. In the past 2 decades, climate change-related increasing glacial stream flow has perturbed long-term stable conditions, which has been associated with lake level rise, the thinning of ice covers, and the expansion of ice-free perimeters, leading to perturbations in light and salinity conditions. Our findings have implications for phytoplankton survival and the response to change scenarios in the light-limited environment of Antarctic ice-covered lakes.
A Link between Species Abundance and Plant Strategies for Semi-Natural Dry Grasslands
Authors: Škornik Sonja , Pipenbaher Nataša
Published: August 2024

Due of the potential of species to determine ecosystem properties, it is important to understand how species abundance influences community assembly. Using vegetation surveys on 35 dry grasslands in north-east Slovenia, we defined dominant (8) and subordinate (61) plant species. They were compared on 14 traits to test for differences in community-weighted mean (CWM) and functional diversity (FD). We found that dominants and subordinates differed strongly in their functional traits. Dominants showed higher leaf dry matter content and a more pronounced stress tolerance strategy and were all clonal with a large proportion of species with rhizomes and a rich bud bank, while other species showed a higher specific leaf area, a longer flowering period and more ruderals. For most traits, FD was higher in subordinates. Our results suggest that dominants drive community structure by limited susceptibility to non-competitive processes. Dominants may have positive effects on subordinates by mitigating environmental stressors. Subordinates are able to assemble together by being dissimilar and use different fine-scale niches that are engineered and homogenised by dominants. Our results show that there are fundamental differences in the relative importance of ecological processes between dominant and subordinate plants in species-rich grasslands, which is also important for their conservational management.
Synthetic Chemicals as Potential Tracers of Impacts of Fracturing Fluids on Groundwater
Authors: Van Stempvoort Dale R. , Brown Susan , Kulasekera Priyantha , Collins Pamela
Published: August 2024

Application of hydraulic fracturing to produce “unconventional” oil and gas from shale formations and other low-permeability geological units has raised concerns about the potential environmental impacts, including potential adverse effects of fracturing fluids (FF) on groundwater. In this study, laboratory batch test experiments and new analytical methods were developed to analyze FF chemicals as potential indicators (tracers) to detect impacts of fracturing fluids on groundwater. The tests, conducted over 101–196 days, included FF with synthetic chemicals (~40,000–4,000,000 µg/L), placed in batches with groundwater and sediment at 5° and 25 °C, along with sterile controls. Using the new methods, measurable concentrations of the FF chemicals were many orders in magnitude lower (~3000 to 3,000,000 X) compared to their concentrations in synthetic fracturing fluids, indicating that these chemicals are excellent candidates as indicators of FF contamination in groundwater, if they are relatively persistent, and not prone to extensive loss by sorption during migration in the subsurface. Variable sorption and degradation of the chemicals was observed in both batch and column tests. Sorption was negligible (sorption coefficient, Kd~0.0) for some synthetic chemicals (polyethylene glycol, ethanolamines, isopropanol, and ethyl hexanol) in some tests. At the other extreme, strong sorption was observed for some of the higher molecular weight cocamido propyl betaine (max Kd = 1.17) and polyethylene glycol (max Kd = 1.12) components, and triethanolamine (max Kd = 0.47) in other tests. Apparent loss by degradation was observed for each chemical in some tests, but negligible in others. The shortest apparent half-lives were for isopropanol and ethyl hexanol at 25 °C (t½ < 11 days), and the most persistent synthetic chemicals were polyethylene glycols (t½ ≥ 182 d) and the ethanolamines (t½ ≥ 212 d). Of the potentially diagnostic FF chemicals investigated, the relatively hydrophilic and persistent lower molecular weight polyethylene glycols are some of the most promising as potential indicators of contamination of groundwater by FF.
Free-Standing, Water-Resistant, and Conductivity-Enhanced PEDOT:PSS Films from In Situ Polymerization of 3-Hydroxymethyl-3-Methyl-Oxetane
Authors: Jorge Sara M. , Santos Luís F. , Ferreira Maria João , Marto-Costa Carolina , Serro Ana Paula , Galvão Adelino M. , Morgado Jorge , Charas Ana
Published: August 2024

Free-standing films based on conducting polymers, such as poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) (PEDOT:PSS), offer many benefits over traditional metal electrodes for applications in flexible electronics. However, to ensure structural integrity when contacting aqueous environments and high levels of electrical conductivity, solution-processed polymers require additives that act as crosslinking agents and conductivity enhancers. In this work, a new approach is presented to fabricate water-resistant free-standing films of PEDOT:PSS and simultaneously increase their conductivity, using an oxetane compound as an additive. It is shown that at moderate temperatures, oxetane polymerizes within the PEDOT:PSS acidic medium, forming hydroxymethyl-substituted polyether compounds that form a network upon crosslinking with PSS. The polymer composite films show self-sustainability, structural stability in aqueous environments, and enhanced conductivity. Finally, the potential of the free-standing films as health-monitoring electrodes, specifically for human electrocardiography, is explored.
Isoconversional Analysis of the Catalytic Pyrolysis of ABS, HIPS, PC and Their Blends with PP and PVC
Authors: Charitopoulou Maria-Anna , Vouvoudi Evangelia C. , Achilias Dimitris S.
Published: August 2024

Thermochemical recycling of plastics in the presence of catalysts is often employed to facilitate the degradation of polymers. The choice of the catalyst is polymer-oriented, while its selection becomes more difficult in the case of polymeric blends. The present investigation studies the catalytic pyrolysis of polymers abundant in waste electric and electronic equipment (WEEE), including poly(acrylonitrile-butadiene-styrene) (ABS), high-impact polystyrene (HIPS) and poly(bisphenol-A carbonate) (PC), along with their blends with polypropylene (PP) and poly(vinyl chloride) (PVC). The aim is to study the kinetic mechanism and estimate the catalysts’ effect on the activation energy of the degradation. The chosen catalysts were Fe2O3 for ABS, Al-MCM-41 for HIPS, Al2O3 for PC, CaO for Blend A (comprising ABS, HIPS, PC and PP) and silicalite for Blend B (comprising ABS, HIPS, PC, PP and PVC). Thermogravimetric experiments were performed in a N2 atmosphere at several heating rates. Information on the degradation mechanism (degradation steps, initial and final degradation temperature, etc.) was attained. It was found that for pure (co)polymers, the catalytic degradation occurred in one-step, whereas in the case of the blends, two steps were required. For the estimation of the activation energy of those degradations, isoconversional kinetic models (integral and differential) were employed. In all cases, the catalysts used were efficient in reducing the estimated Eα, compared to the values of Eα obtained from conventional pyrolysis.
Adaptive Neuro-Fuzzy Inference System and Artificial Neural Network Models for Predicting Time-Dependent Moisture Levels in Hazelnut Shells (Corylus avellana L.) and Prina (Oleae europaeae L.)
Authors: Bulus Halil Nusret
Published: August 2024

Nowadays, in parallel with the rapid increase in industrialization and human population, a significant increase in all types of waste, especially domestic, industrial, and agricultural waste, can be observed. In this study, microwave drying, one of the disposal methods for agricultural waste, such as prina and hazelnut shell, was performed. To reduce the time, energy, and cost spent on drying processes, two recently prominent machine learning prediction methods (Artificial Neural Network (ANN) and Adaptive Neuro-Fuzzy Inference System (ANFIS)) were applied. In this study, our aim is to model the disposal of waste using artificial intelligence techniques, especially considering the importance of environmental pollution in today’s context. Microwave power values of 120, 350, and 460 W were used for 100 g of hazelnut shell, and 90 W, 360 W, and 600 W were used for 7 mm thickness of prina. Both ANN and ANFIS approaches were applied to a dataset obtained from the calculation of moisture content and drying rate values. It was observed that the ANFIS and ANN models were applicable for predicting moisture levels, but not applicable for predicting drying rates. When the coefficient of determination (R2), Root Mean Square Error (RMSE) and Mean Absolute Percentage Error (MAPE) values for moisture level are examined both for ANN and ANFIS models’ predictions, it is seen that the R2 value is between 0.981340 and 0.999999, the RMSE value is between 0.000012 and 0.015010 and the MAPE value is between 0.034268 and 23.833481.
Average Carbon Number Analysis and Relationship with Octane Number and PIONA Analysis of Premium and Regular Gasoline Expended in Ecuador
Authors: Pazmiño-Viteri Katherine , Cabezas-Terán Katty , Echeverría Daniel , Cabrera Marcelo , Taco-Vásquez Sebastián
Published: August 2024

The quality of fuel depends on its chemical composition, which influences engine performance. Gas chromatography, a cornerstone of global oil and fuel R&D, remains crucial for ensuring the quality of petroleum products and regulatory compliance. Scientists use the most accurate analysis (PIONA) as a tool derived from gas chromatography coupled with mass spectrometry to identify and quantify hydrocarbons that influence resistance to detonation, which is determined by the research octane number (RON). This study introduces the “average carbon number (ACN)”, calculated from the molar chemical composition of commercial gasoline samples sold in Ecuador (Extra gasoline and Súper gasoline). A quantitative comparison of the ACN with techniques applied using standardized international procedures reveals that the ACN characterizes gasoline samples by providing insight into the distribution shape of carbon graphs. A comprehensive statistical analysis demonstrates the potential usefulness of ACN in characterizing fuel composition, highlighting its relevance in broader fuel quality assessments without the need for carbon distribution plots.
Evaluation of the Quality of ChatGPT’s Responses to Top 20 Questions about Robotic Hip and Knee Arthroplasty: Findings, Perspectives and Critical Remarks on Healthcare Education
Authors: Venosa Michele , Calvisi Vittorio , Iademarco Giulio , Romanini Emilio , Ciminello Enrico , Cerciello Simone , Logroscino Giandomenico
Published: August 2024

Robotic-assisted hip and knee arthroplasty represents significant advancements in orthopedic surgery. Artificial intelligence (AI)-driven chatbots, such as ChatGPT, could play a significant role in healthcare education. This study aims to evaluate the quality of responses provided by ChatGPT to the top 20 questions concerning robotic-assisted hip and knee arthroplasty. We have asked ChatGPT to select the top 20 questions on Google concerning robotic hip and knee arthroplasty and to provide a detailed answer to each of them. The accuracy and completeness of the information provided were examined by three orthopedic surgeons with scientific and clinical experience in hip- and knee-replacement surgery. The accuracy was assessed through a 5-point Likert scale (from 1—completely incorrect to 5—correct); the completeness through a 4-point Likert scale (from 0—comprehensiveness not assessable for completely incorrect answers to 3—exhaustive information) on two different occasions to ensure the consistency of the assessment. Our analysis reveals that ChatGPT provides a relatively high degree of accuracy; moreover, the explanations can be considered satisfying, especially for factual questions. The findings suggest that ChatGPT can serve as a valuable initial resource for general information on robotic hip and knee arthroplasty but the integration with human expertise remains essential.