DETERMINATION OF BIOFILM FORMATION ABILITY OF REPRESENTATIVES OF MICROBIOCENOSE OF BATTLE WOUNDS
Authors:   SE ", Kutasevych Yanina ,   Institute of Dermatology and Venereology of the NAMS of Ukraine", Dzhoraieva S. K. ,   SE "Institute of Dermatology and Venereology of the NAMS of Ukraine", Kondakova G. K. ,   SE "Institute of Dermatology and Venereology of the NAMS of Ukraine", Khoroshun E. M. ,   Military Medical Clinical Center of the Northern Region of the Command of the Medical Forces of the Armed Forces of Ukraine, Lyapunov N. A. ,   State Scientific Institution "Institute for Single Crystals" of NAS of Ukraine, Goncharenko V. V. ,   SE "Institute of Dermatology and Venereology of the NAMS of Ukraine", Oliinyk I. O. ,   SE "Institute of Dermatology and Venereology of the NAMS of Ukraine", Birukov M. S. ,   Military Medical Clinical Center of the Northern Region of the Command of the Medical Forces of the Armed Forces of Ukraine, Kupriyanchuk V. V. ,   Military Medical Clinical Center of the Northern Region of the Command of the Medical Forces of the Armed Forces of Ukraine, Shipilov S. A. ,   Military Medical Clinical Center of the Northern Region of the Command of the Medical Forces of the Armed Forces of Ukraine
Published: November 2023

The article presents experimental data on the study of the ability to form biofilms of clinical strains of bacteria isolated from combat wounds. The search results regarding the determination of MBС dioxidin in relation to clinical strains – representatives of different taxonomic groups are provided. the dynamics of formation of resistance to dioxidin was determined using the reference strains S. aureus ATCC25923, P. aeruginosa ATCC27853. The purpose evaluation of the ability to form biofilms of dominant representatives of the microbiocenosis of combat wounds and the effect of dioxidin on their expression level Materials and methods. In general, the ability to form biofilms of 20 clinical strains of pathogens was determined (S. haemolyticus – 8 samples; K. pneumoniae – 4 samples; E. coli, P. aeruginosa and S epidermidis – 2 samples each and C. amalonaticus and S.aureus – each one) and 3 reference cultures: E. сoli ATCC25922, S. aureus ATCC25923, P. aeruginosa ATCC27853 and the effect on their formation of dioxidin solution with determination of MBK against planktonic cells of these strains Research results: It was established that both reference and clinical strains of microorganisms were characterized by medium and high ability to form biofilms. According to the results of the conducted studies, it was shown that the drug dioxydin showed antimicrobial activity against the majority of planktonic cells of all gram-negative bacteria used in the work, with an MBC from 0.04 to 0.63 mg/ml. The lowest level of activity of the drug was noted against strains of staphylococci (MBC from 1.25 to >2.5 mg/ml), except for planktonic cells of strain S. aureus № . 2093, for which the MBC of dioxidin was 5 mg/ml. In the course of the study, an analysis of the dynamics of the formation of resistance to dioxidin in microorganisms of different taxonomic groups was carried out. S. aureus ATCC25923 strain formed a variant resistant to dioxidin (MBK ≥ 10 mg/ml), while P. aeruginosa ATCC27853 strains did not form such variants, which indicates the possibility of using dioxidin in the long-term therapy of wound infection. Conclusion. The obtained research results demonstrate the microbiological effectiveness of dioxidin, which can be used in the complex treatment of microbial complications of wound injuries