bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Pri-miRNA Stem-Loop Interacting Proteins in Plants Using a Modified Version of the Csy4 CRISPR Endonuclease
Authors: Lüders Janina , Winkel Andreas R. , Reichel Marlene , Bitterer Valentin W. , Scheibe Marion , Widmann Christiane , Butter Falk , Köster Tino
Published: August 2022

Regulation at the RNA level by RNA-binding proteins (RBPs) and microRNAs (miRNAs) is key to coordinating eukaryotic gene expression. In plants, the importance of miRNAs is highlighted by severe developmental defects in mutants impaired in miRNA biogenesis. MiRNAs are processed from long primary-microRNAs (pri-miRNAs) with internal stem-loop structures by endonucleolytic cleavage. The highly structured stem-loops constitute the basis for the extensive regulation of miRNA biogenesis through interaction with RBPs. However, trans-acting regulators of the biogenesis of specific miRNAs are largely unknown in plants. Therefore, we exploit an RNA-centric approach based on modified versions of the conditional CRISPR nuclease Csy4* to pull down interactors of the Arabidopsis pri-miR398b stem-loop (pri-miR398b-SL) in vitro. We designed three epitope-tagged versions of the inactive Csy4* for the immobilization of the protein together with the pri-miR398b-SL bait on high affinity matrices. After incubation with nucleoplasmic extracts from Arabidopsis and extensive washing, pri-miR398b-SL, along with its specifically bound proteins, were released by re-activating the cleavage activity of the Csy4* upon the addition of imidazole. Co-purified proteins were identified via quantitative mass spectrometry and data sets were compared. In total, we identified more than 400 different proteins, of which 180 are co-purified in at least two out of three independent Csy4*-based RNA pulldowns. Among those, the glycine-rich RNA-binding protein AtRZ-1a was identified in all pulldowns. To analyze the role of AtRZ-1a in miRNA biogenesis, we determined the miR398 expression level in the atrz-1a mutant. Indeed, the absence of AtRZ-1a caused a decrease in the steady-state level of mature miR398 with a concomitant reduction in pri-miR398b levels. Overall, we show that our modified Csy4*-based RNA pulldown strategy is suitable to identify new trans-acting regulators of miRNA biogenesis and provides new insights into the post-transcriptional regulation of miRNA processing by plant RBPs.
(-)-Epicatechin Is a Biased Ligand of Apelin Receptor
Authors: Portilla-Martínez Andrés , Ortiz-Flores Miguel Ángel , Meaney Eduardo , Villarreal Francisco , Nájera Nayelli , Ceballos Guillermo
Published: August 2022

(-)-Epicatechin (EC) is part of a large family of biomolecules called flavonoids and is widely distributed in the plant kingdom. Several studies have shown the beneficial effects of EC consumption. Many of these reported effects are exerted by activating the signaling pathways associated with the activation of two specific receptors: the G protein-coupled estrogen receptor (GPER), a transmembrane receptor, and the pregnane X receptor (PXR), which is a nuclear receptor. However, the effects of EC are so diverse that these two receptors cannot describe the complete phenomenon. The apelin receptor or APLNR is classified within the G protein-coupled receptor (GPCR) family, and is capable of activating the G protein canonical pathways and the β-arrestin transducer, which participates in the phenomenon of receptor desensitization and internalization. β-arrestin gained interest in selective pharmacology and mediators of the so-called “biased agonism”. With molecular dynamics (MD) and in vitro assays, we demonstrate how EC can recruit the β-arrestin in the active conformation of the APLN receptor acting as a biased agonist.
Discovery of the Key Mutation Site Influencing the Thermostability of Thermomyces lanuginosus Lipase by Rosetta Design Programs
Authors: Zhu Enheng , Xiang Xia , Wan Sidi , Miao Huabiao , Han Nanyu , Huang Zunxi
Published: August 2022

Lipases are remarkable biocatalysts and are broadly applied in many industry fields because of their versatile catalytic capabilities. Considering the harsh biotechnological treatment of industrial processes, the activities of lipase products are required to be maintained under extreme conditions. In our current study, Gibbs free energy calculations were performed to predict potent thermostable Thermomyces lanuginosus lipase (TLL) variants by Rosetta design programs. The calculating results suggest that engineering on R209 may greatly influence TLL thermostability. Accordingly, ten TLL mutants substituted R209 were generated and verified. We demonstrate that three out of ten mutants (R209H, R209M, and R209I) exhibit increased optimum reaction temperatures, melting temperatures, and thermal tolerances. Based on molecular dynamics simulation analysis, we show that the stable hydrogen bonding interaction between H198 and N247 stabilizes the local configuration of the 250-loop in the three R209 mutants, which may further contribute to higher rigidity and improved enzymatic thermostability. Our study provides novel insights into a single residue, R209, and the 250-loop, which were reported for the first time in modulating the thermostability of TLL. Additionally, the resultant R209 variants generated in this study might be promising candidates for future-industrial applications.
The Joint Influence of Tl+ and Thiol-Modifying Agents on Rat Liver Mitochondrial Parameters In Vitro
Authors: Korotkov Sergey M. , Novozhilov Artemy V.
Published: August 2022

Recent data have shown that the mitochondrial permeability transition pore (MPTP) is the complex of the Ca2+-modified adenine nucleotide translocase (ANT) and the Ca2+-modified ATP synthase. We found in a previous study that ANT conformational changes may be involved in Tl+-induced MPTP opening in the inner membrane of Ca2+-loaded rat liver mitochondria. In this study, the effects of thiol-modifying agents (eosin-5-maleimide (EMA), fluorescein isothiocyanate (FITC), Cu(o-phenanthroline)2 (Cu(OP)2), and embelin (Emb)), and MPTP inhibitors (ADP, cyclosporine A (CsA), n-ethylmaleimide (NEM), and trifluoperazine (TFP)) on MPTP opening were tested simultaneously with increases in swelling, membrane potential (ΔΨmito) decline, decreases in state 3, 4, and 3UDNP (2,4-dinitrophenol-uncoupled) respiration, and changes in the inner membrane free thiol group content. The effects of these thiol-modifying agents on the studied mitochondrial characteristics were multidirectional and showed a clear dependence on their concentration. This research suggests that Tl+-induced MPTP opening in the inner membrane of calcium-loaded mitochondria may be caused by the interaction of used reagents (EMA, FITC, Emb, Cu(OP)2) with active groups of ANT, the mitochondrial phosphate carrier (PiC) and the mitochondrial respiratory chain complexes. This study provides further insight into the causes of thallium toxicity and may be useful in the development of new treatments for thallium poisoning.
Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD)
Authors: Simoncini Stéphanie , Toupance Simon , Labat Carlos , Gautier Sylvie , Dumoulin Chloé , Arnaud Laurent , Stathopoulou Maria G. , Visvikis-Siest Sophie , Rossi Pascal M. , Benetos Athanase , Dignat-George Françoise , Sabatier Florence
Published: August 2022

Endothelial dysfunction is a key factor in atherosclerosis. However, the link between endothelial repair and severity of atherosclerotic cardiovascular disease (ASCVD) is unclear. This study investigates the relationship between ASCVD, markers of inflammation, and circulating endothelial progenitor cells, namely hematopoietic cells with paracrine angiogenic activity and endothelial colony forming cells (ECFC). Two hundred and forty-three subjects from the TELARTA study were classified according to the presence of clinical atherosclerotic disease. ASCVD severity was assessed by the number of involved vascular territories. Flow cytometry was used to numerate circulating progenitor cells (PC) expressing CD34 and those co-expressing CD45, CD34, and KDR. Peripheral blood mononuclear cells ex vivo culture methods were used to determine ECFC and Colony Forming Unit- endothelial cells (CFU-EC). The ECFC subpopulation was analyzed for proliferation, senescence, and vasculogenic properties. Plasma levels of IL-6 and VEGF-A were measured using Cytokine Array. Despite an increased number of circulating precursors in ASCVD patients, ASCVD impaired the colony forming capacity and the angiogenic properties of ECFC in a severity-dependent manner. Alteration of ECFC was associated with increased senescent phenotype and IL-6 levels. Our study demonstrates a decrease in ECFC repair capacity according to ASCVD severity in an inflammatory and senescence-associated secretory phenotype context.
Chronic Venous Disease during Pregnancy Causes a Systematic Increase in Maternal and Fetal Proinflammatory Markers
Authors: Ortega Miguel A. , Gómez-Lahoz Ana M. , Sánchez-Trujillo Lara , Fraile-Martinez Oscar , García-Montero Cielo , Guijarro Luis G. , Bravo Coral , De Leon-Luis Juan A. , Saz Jose V. , Bujan Julia , García-Honduvilla Natalio , Monserrat Jorge , Alvarez-Mon Melchor
Published: August 2022

Chronic venous disease (CVD) is a common vascular disorder characterized by increased venous hypertension and insufficient venous return from the lower limbs. Pregnancy is a high-risk situation for developing CVD. Approximately a third of the women will develop this condition during pregnancy, and similarly to arterial hypertensive disorders, previous evidence has described a plethora of alterations in placental structure and function in women with pregnancy-induced CVD. It is widely known that arterial-induced placenta dysfunction is accompanied by an important immune system alteration along with increased inflammatory markers, which may provide detrimental consequences for the women and their offspring. However, to our knowledge, there are still no data collected regarding cytokine profiling in women with pregnancy-induced CVD. Thus, the aim of the present work was to examine cytokine signatures in the serum of pregnant women (PW) with CVD and their newborns (NB). This study was conducted through a multiplex technique in 62 PW with pregnancy-induced CVD in comparison to 52 PW without CVD (HC) as well as their NB. Our results show significant alterations in a broad spectrum of inflammatory cytokines (IL-6, IL-12, TNF-α, IL-10, IL-13, IL-2, IL-7, IFN-γ, IL-4, IL-5, IL-21, IL-23, GM-CSF, chemokines (fractalkine), MIP-3α, and MIP-1β). Overall, we demonstrate that pregnancy-induced CVD is associated with a proinflammatory environment, therefore highlighting the potentially alarming consequences of this condition for maternal and fetal wellbeing.
Microbial Community, Metabolic Potential and Seasonality of Endosphere Microbiota Associated with Leaves of the Bioenergy Tree Paulownia elongata × fortunei
Authors: Woźniak Małgorzata , Gałązka Anna , Marzec-Grządziel Anna , Frąc Magdalena
Published: August 2022

The microbial structure and metabolic function of plant-associated endophytes play a key role in the ecology of various environments, including trees. Here, the structure and functional profiles of the endophytic bacterial community, associated with Paulownia elongata × fortunei, in correlation with seasonality, were evaluated using Biolog EcoPlates. Biolog EcoPlates was used to analyse the functional diversity of the microbiome. The total communities of leaf endophyte communities were investigated using 16S rRNA V5–V7 region amplicon deep sequencing via Illumina MiSeq. Community level physiological profiling (CLPP) analysis by the Biolog EcoPlate™ assay revealed that the carboxylic acids (19.67–36.18%) and amino acids (23.95–35.66%) were preferred by all by all communities, whereas amines and amides (0.38–9.46%) were least used. Seasonal differences in substrate use were also found. Based on the sequencing data, mainly phyla Proteobacteria (18.4–97.1%) and Actinobacteria (2.29–78.7%) were identified. A core microbiome could be found in leaf-associated endophytic communities in trees growing in different locations. This work demonstrates the application of Biolog EcoPlates in studies of the functional diversity of microbial communities in a niche other than soil and shows how it can be applied to the functional analyses of endomicrobiomes. This research can contribute to the popularisation of Biolog EcoPlates for the functional analysis of the endomicrobiome. This study confirms that the analysis of the structure and function of the plant endophytic microbiome plays a key role in the health control and the development of management strategies on bioenergy tree plantations.
Web-MCOT Server for Motif Co-Occurrence Search in ChIP-Seq Data
Authors: Levitsky Victor G. , Mukhin Alexey M. , Oshchepkov Dmitry Yu. , Zemlyanskaya Elena V. , Lashin Sergey A.
Published: August 2022

(1) Background: The widespread application of ChIP-seq technology requires annotation of cis-regulatory modules through the search of co-occurred motifs. (2) Methods: We present the web server Motifs Co-Occurrence Tool (Web-MCOT) that for a single ChIP-seq dataset detects the composite elements (CEs) or overrepresented homo- and heterotypic pairs of motifs with spacers and overlaps, with any mutual orientations, uncovering various similarities to recognition models within pairs of motifs. The first (Anchor) motif in CEs respects the target transcription factor of the ChIP-seq experiment, while the second one (Partner) can be defined either by a user or a public library of Partner motifs being processed. (3) Results: Web-MCOT computes the significances of CEs without reference to motif conservation and those with more conserved Partner and Anchor motifs. Graphic results show histograms of CE abundance depending on orientations of motifs, overlap and spacer lengths; logos of the most common CE structural types with an overlap of motifs, and heatmaps depicting the abundance of CEs with one motif possessing higher conservation than another. (4) Conclusions: Novel capacities of Web-MCOT allow retrieving from a single ChIP-seq dataset with maximal information on the co-occurrence of motifs and potentiates planning of next ChIP-seq experiments.
SARS-CoV-2 and Immunity: Natural Infection Compared with Vaccination
Authors: Vespa Simone , Simeone Pasquale , Catitti Giulia , Buca Davide , De Bellis Domenico , Pierdomenico Laura , Pieragostino Damiana , Cicalini Ilaria , Del Boccio Piero , Natale Luca , Owens Trevor , Khorooshi Reza , De Laurenzi Vincenzo , Stuppia Liborio , Lanuti Paola
Published: August 2022

Recently, the protective and/or pathological role of virus-specific T cells in SARS-CoV-2 infection has been the focus of many studies. We investigated the anti-spike IgG levels and SARS-CoV-2-specific T cells in 125 donors (90 vaccinated with four different vaccine platforms, 16 individuals with a previous natural infection, and 19 not vaccinated donors who did not report previous SARS-CoV-2 infections). Our data show that anti-spike IgG titers were similar between naturally infected subjects and those vaccinated with adenoviral vector vaccines. Of note, all immunized donors produced memory CD4+ and/or CD8+ T cells. A sustained polyfunctionality of SARS-CoV-2-specific T cells in all immunized donors was also demonstrated. Altogether, our data suggest that the natural infection produces an overall response like that induced by vaccination. Therefore, this detailed immunological evaluation may be relevant for other vaccine efforts especially for the monitoring of novel vaccines effective against emerging virus variants.
Comparison of Sputum Treated with Power Ultrasound and Routine NALC-NaOH Methods for Mycobacterial Culture: A Prospective Study
Authors: Wang Junling , Wang Yan , Ling Xiaojie , Zhang Zhenjin , Deng Yunfeng , Tian Peng
Published: August 2022

Mycobacterial culture remains the gold standard for the diagnosis of active tuberculosis. However, an appropriate digestion and decontamination method is essential for the effective recovery of tubercle bacilli in culture. The study was designed to compare the efficacy of sputum treated with power ultrasound (PU) and routine NALC-NaOH methods for mycobacterial culture from clinically suspected cases of pulmonary tuberculosis. To evaluate the PU and routine NALC-NaOH methods, sputum specimens (n = 597) were studied (culturing on MGIT 960), and the performances were compared. Of the 597 samples, 89 (14.91%) sputum samples treated with the NaOH-NALC method were mycobacterial culture positive, including Mycobacterium tuberculosis (M.TB; n = 77, 12.90%) and nontuberculous mycobacteria (NTM; n = 12, 2.01%). One hundred and ten (18.43%) sputum samples treated with the PU method were culture positive, including M.TB (n = 87, 14.57%) and NTM (n = 23, 3.85%). The PU method detected 10 additional cases of M.TB and 11 additional cases of NTM when compared to the NALC-NaOH method. Statistical analysis showed that a significant difference was found in the culture-positive ratio of M.TB and NTM between the two method groups (p < 0.05). Compared with that of the NALC-NaOH method (8.04%), sputum treated with PU method (4.86%) had a significantly lower contamination rate (p < 0.05). In conclusion, our data indicate that, compared with the NALC-NaOH method, the PU method is a rapid and effective approach for mycobacterial culture when detecting active TB. However, its accurate mechanism has not been well addressed, and further investigation is still required.
Endobronchial Ultrasound-Guided Transbronchial Forceps Biopsy: A Retrospective Bicentric Study Using the Olympus 1.5 mm Mini-Forceps
Authors: Rüber Fabienne , Wiederkehr Gilles , Steinack Carolin , Höller Sylvia , Bode Peter Karl , Kölbener Fabian , Franzen Daniel Peter
Published: August 2022

When evaluating mediastinal/hilar lymphadenopathy (LAD) or masses, guidelines recommend endobronchial ultrasound (EBUS)-guided transbronchial needle aspiration (TBNA) as an initial technique for tissue analysis and diagnosis. However, owing to the small sample size obtained by needle aspiration, its diagnostic yield (DY) is limited. EBUS transbronchial forceps biopsy (TBFB) used as a complimentary technique to EBUS-TBNA might allow for better histopathological evaluation, thus improving DY. In this retrospective bicentric study, we assessed the DY and safety of an EBUS-guided 1.5 mm mini-forceps biopsy combined with EBUS-TBNA for the diagnosis of mediastinal/hilar LAD or masses compared to EBUS-TBNA alone. In total, 105 patients were enrolled. The overall DY was 61.9% and 85.7% for TBNA alone and EBUS-TBNA combined with EBUS-TBFB, respectively (p < 0.001). While the combined approach was associated with a significantly higher DY for lung cancer diagnosis (97.1% vs. 76.5%, p = 0.016) and sarcoidosis (85.2% vs. 44.4%, p = 0.001), no significant differences in DY were calculated for subgroups with smaller sample sizes such as lymphoma. No major adverse events were observed. Using a 1.5 mm mini-forceps is a safe and feasible technique for biopsy of mediastinal or hilar LAD or masses with superior overall DY compared to EBUS-TBNA as a standalone technique.
Management of High-Risk Hypercholesterolemic Patients and PCSK9 Inhibitors Reimbursement Policies: Data from a Cohort of Italian Hypercholesterolemic Outpatients
Authors: Fogacci Federica , Giovannini Marina , Grandi Elisa , Imbalzano Egidio , Degli Esposti Daniela , Borghi Claudio , Cicero Arrigo F. G.
Published: August 2022

Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors are effective and safe lipid-lowering treatments (LLT). The primary endpoint of the study was to assess the prevalence of patients eligible for treatment with PCSK9 inhibitors in a real-life clinical setting in Italy before and after the recent enlargement of reimbursement criteria. For this study, we consecutively considered the clinical record forms of 6231 outpatients consecutively admitted at the Lipid Clinic of the University Hospital of Bologna (Italy). Patients were stratified according to whether they were allowed or not allowed to access to treatment with PCSK9 inhibitors based on national prescription criteria and reimbursement rules issued by the Italian Medicines Agency (AIFA). According to the indications of the European Medicines Agency (EMA), 986 patients were candidates to treatment with PCSK9 inhibitors. However, following the prescription criteria issued by AIFA, only 180 patients were allowed to access to PCSK9 inhibitors before reimbursement criteria enlargement while 322 (+14.4%) with the current ones. Based on our observations, low-cost tailored therapeutic interventions for individual patients can significantly reduce the number of patients potentially needing treatment with PCSK9 inhibitors among those who are not allowed to access to the treatment. The application of enlarged reimbursement criteria for PCSK9 inhibitors could mildly improve possibility to adequately manage high-risk hypercholesterolemic subjects in the setting of an outpatient lipid clinic.
Evaluating the Medication Regimen Complexity Score as a Predictor of Clinical Outcomes in the Critically Ill
Authors: Al-Mamun Mohammad A. , Strock Jacob , Sharker Yushuf , Shawwa Khaled , Schmidt Rebecca , Slain Douglas , Sakhuja Ankit , Brothers Todd N.
Published: August 2022

Background: Medication Regimen Complexity (MRC) refers to the combination of medication classes, dosages, and frequencies. The objective of this study was to examine the relationship between the scores of different MRC tools and the clinical outcomes. Methods: We conducted a retrospective cohort study at Roger William Medical Center, Providence, Rhode Island, which included 317 adult patients admitted to the intensive care unit (ICU) between 1 February 2020 and 30 August 2020. MRC was assessed using the MRC Index (MRCI) and MRC for the Intensive Care Unit (MRC-ICU). A multivariable logistic regression model was used to identify associations among MRC scores, clinical outcomes, and a logistic classifier to predict clinical outcomes. Results: Higher MRC scores were associated with increased mortality, a longer ICU length of stay (LOS), and the need for mechanical ventilation (MV). MRC-ICU scores at 24 h were significantly (p < 0.001) associated with increased ICU mortality, LOS, and MV, with ORs of 1.12 (95% CI: 1.06–1.19), 1.17 (1.1–1.24), and 1.21 (1.14–1.29), respectively. Mortality prediction was similar using both scoring tools (AUC: 0.88 [0.75–0.97] vs. 0.88 [0.76–0.97]. The model with 15 medication classes outperformed others in predicting the ICU LOS and the need for MV with AUCs of 0.82 (0.71–0.93) and 0.87 (0.77–0.96), respectively. Conclusion: Our results demonstrated that both MRC scores were associated with poorer clinical outcomes. The incorporation of MRC scores in real-time therapeutic decision making can aid clinicians to prescribe safer alternatives.
Study on Sloshing Characteristics in a Liquid Cargo Tank under Combination Excitation
Authors: Zhang Qiong , Shui Bo , Zhu Hanhua
Published: August 2022

Sloshing is a common flow phenomenon in liquid cargo tanks and has a great negative impact on the stability and safety of ship navigation. It is important to understand the sloshing process of tanks under the excitation of complex external conditions for the transportation of liquid cargo. In this paper, the sloshing characteristics of a liquid cargo tank are studied under the combination excitation conditions of roll and surge. The pressure distribution characteristics at different positions of the cargo tank are discussed, along with the influence of different excitation conditions on the pressure of the cargo tank. The results show that under the condition of combination excitation, the fluid sloshes along the diagonal direction of the tank, and the peak liquid height and peak pressure are located on the diagonal corner of the tank. The peak pressure at the lowest point on the diagonal of the tank is proportional to the amplitude of the roll angle and surge, and the change in roll angle amplitude has a significant impact on the pressure and liquid height at different positions.
Tropical and Polar Oceanic Influences on the Cold Extremes in East Asia: Implications of the Cold Surges in 2020/2021 Winter
Authors: Tan Xiaoyu , Zhong Linhao , Mu Lin , Gong Zhaohui
Published: August 2022

East-Asia winter cooling and the associated atmospheric and oceanic influences were investigated based on the wintertime daily temperature and circulation fields during 1950–2020. Both the case study on the 2020/2021 cold surge and the large-sample clustering in the recent 71 winters extracted similar circulation signatures for East-Asia cooling, which are featured by the blocking-related anticyclonic circulation in North Eurasia, large-scale mid-to-high-latitude wave trains, decrease in the sea surface temperature (SST) in tropical Pacific, and the sea-ice cover (SIC) reduction in the Barents and Kara Seas (BKS). From the joint clustering of Eurasian circulation and temperature, two circulation modes that have a cooling effect on East Asia account for 41% of winter days. One of the two modes is characterized by the cyclonic circulation over Northeast Asia coupled with a southward-extending negative-phase Arctic Oscillation (AO−), whose cooling effect is mainly concentrated in central Siberia. The other cooling mode, featuring an anticyclonic circulation over the Urals and AO+ in middle-to-high latitudes, has a relatively stronger cooling effect on lower latitudes, including Mongolia and North China. In general, the occurrences of the mode with warming/cooling effect on East Asia show an overall downward/upward trend. The two cooling modes are significantly influenced by the La Niña-type SST anomaly and reduced SIC in BKS through large-scale wave trains, of which the tropical oceanic forcing mainly acts as a climatic background. Furthermore, the polar forcing is more tightly bound to internal atmospheric variability. Therefore, the tropical SST tends to exert impact over a seasonal scale, but the SIC influence is more significant below the intraseasonal scale; moreover, the synergy between the tropical and polar oceanic forcing can increase the East-Asia cooling days by 3–4% and cold extremes by 5%, mainly through enhancing the AO-related circulation mode.
Beauveria bassiana (Hypocreales: Clavicipitaceae) Volatile Organic Compounds (VOCs) Repel Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)
Authors: Jalinas Johari , Lopez-Moya Federico , Marhuenda-Egea Frutos C. , Lopez-Llorca Luis Vicente
Published: August 2022

The entomopathogenic fungus Beauveria bassiana (Bb) is used to control the red palm weevil (RPW) Rhyncophorus ferrugineus (Oliver). Beuveria bassiana can infect and kill all developmental stages of RPW. We found that a solid formulate of B. bassiana isolate 203 (Bb203; CBS 121097), obtained from naturally infected RPW adults, repels RPW females. Fungi, and entomopathogens in particular, can produce volatile organic compounds (VOCs). VOCs from Bb203 were analyzed using gas chromatography-mass spectrometry (GC-MS). GC-MS identified more than 15 VOCs in B. bassiana not present in uninoculated (control) formulate. Both ethenyl benzene and benzothiazole B. bassiana VOCs can repel RPW females. Our findings suggest that B. bassiana and its VOCs can be used for sustainable management of RPW. They could act complementarily to avoid RPW infestation in palms.
Long-Time-Series Evolution and Ecological Effects of Coastline Length in Coastal Zone: A Case Study of the Circum-Bohai Coastal Zone, China
Authors: Tang Shisi , Song Laixi , Wan Shiqi , Wang Yafei , Jiang Yazhen , Liao Jinfeng
Published: August 2022

Under the joint influence of climate change and human production and living activities on land and sea, the morphology and use function of the coastline have undergone notable changes, triggering more significant environmental and ecological effects. In this paper, we quantified the evolution characteristics of the coastline over the past 30 years and measured the possible environmental pollution and ecological degradation by means of spatiotemporal coupling analysis in terms of spatial form and land-use function. The Chinese Bohai Rim region was taken as an example, and the Google Earth Engine was applied to achieve a long time series of interannually continuous land use function classification of the coastal zone. The study shows that: (1) from 1987–2020, the coastline of Bohai Rim showed an overall trend of seaward expansion, with the length of the coastline increasing by 15.6%, most significantly from 2003 to 2011; (2) the proportion of construction function coastline increased from 14.66% to 42.8%, while the proportion of cropland coastline decreased from 52.01% to 18.16% during 2000–2020, with the natural ecological coastline decreasing in the early stage and recovering in the later stage, thus becoming more stable overall; (3) 82.73% of coastal districts and counties showed a linear correlation between changes in coastline function and water quality level, and 77.8% showed a consistent degree of change of coastline function and loss of ecological land, indicating that changes in the coastline have triggered significant problems in terms of environmental pollution and ecological degradation. With the exception of localized areas such as the Yellow River Delta, where coastline change is somewhat more significantly influenced by environmental climate change, most other coastline changes are primarily due to human land-based development; therefore, coastal policy constraints bring obvious mitigation.
Microwave versus Conventional Sintering of NiTi Alloys Processed by Mechanical Alloying
Authors: Teixeira Rodolfo da Silva , Oliveira Rebeca Vieira de , Rodrigues Patrícia Freitas , Mascarenhas João , Neves Filipe Carlos Figueiredo Pereira , Paula Andersan dos Santos
Published: August 2022

The present study shows a comparison between two sintering processes, microwave and conventional sintering, for the manufacture of NiTi porous specimens starting from powder mixtures of nickel and titanium hydrogenation–dehydrogenation (HDH) milled by mechanical alloying for a short time (25 min). The samples were sintered at 850 °C for 15 min and 120 min, respectively. Both samples exhibited porosity, and the pore size results are within the range of the human bone. The NiTi intermetallic compound (B2, R-phase, and B19′) was detected in both sintered samples through X-ray diffraction (XRD) and electron backscattering diffraction (EBSD) on scanning electron microscopic (SEM). Two-step phase transformation occurred in both sintering processes with cooling and heating, the latter occurring with an overlap of the peaks, according to the differential scanning calorimetry (DSC) results. From scanning electron microscopy/electron backscatter diffraction, the R-phase and B2/B19′ were detected in microwave and conventional sintering, respectively. The instrumented ultramicrohardness results show the highest elastic work values for the conventionally sintered sample. It was observed throughout this investigation that using mechanical alloying (MA) powders enabled, in both sintering processes, good results, such as intermetallic formation and densification in the range for biomedical applications.
Abnormal Twinning Behavior Induced by Local Stress in Magnesium
Authors: Shi Dongfeng , Zhang Jin
Published: August 2022

This study investigated the twinning behavior with increasing compressive strain in rolled AZ31 alloy. With that purpose, a polycrystalline structure with an average grain size of 30 μm was utilized to perform the uniaxial compression tests. Microstructure evolution was traced by in situ electron backscattered diffraction (EBSD). Multiple primary twin variants and extension double twins were observed in the same grain. A comprehensive analysis of kernel average misorientation (KAM) and Schmid factor (SF) revealed that the nucleation of twins in one special grain is not only based on the SF criterion, but that it is also strongly influenced by surrounding grains. Moreover, the existing primary twins modified the inner and outer strain distribution close to the twin boundaries. With continued compression, the strain inside the primary twins stimulated the nucleation of double twins, while the strain in the matrixes facilitated twin growth. Therefore, the primary twin growth and the new nucleation of secondary twins could take place simultaneously in the same twinning system to meet the requirements of strain accommodation. Twinning behaviors are controlled by the combined effect of the Schmid factor, strain accommodation between surrounding grains, and variation in the local stress state. The local stress exceeded the critical resolved shear stress (CRSS), implying that twin nucleation is possible. Hence, the twinning process tends to be a response of the local stress rather than the applied stress.
N′-Substituted 4-Phenylpicolinohydrazonamides with Thiosemicarbazone Moiety as New Potential Antitubercular Agents: Synthesis, Structure and Evaluation of Antimicrobial Activity
Authors: Gobis Katarzyna , Szczesio Małgorzata , Olczak Andrzej , Mazerant-Politowicz Ida , Ziembicka Dagmara , Pacholczyk-Sienicka Barbara , Augustynowicz-Kopeć Ewa , Głogowska Agnieszka , Korona-Głowniak Izabela , Fruziński Andrzej
Published: August 2022

Three new 4-phenylpicolin derivatives with a thiosemicarbazone structure were synthesized and evaluated for tuberculostatic activity. The compounds were obtained by the condensation of methyl 4-phenylpicolonimidate with the corresponding cycloalkylamino-1-carbothiohydrazides. The 1H NMR temperature spectra obtained showed proton lability at the nitrogen atom N2, and X-ray crystallography confirmed the zwitterionic structure of all products. ADME calculations indicate that the compounds can be tested as future drugs. All compounds were absorbed in the gastrointestinal tract. All compounds also showed very good tuberculostatic activity (MIC 3.1–12.5 µg/mL). Derivative 1b showed the best selectivity for M. tuberculosis compared to the other pathogenic species tested. The study has allowed the emergence of imine derivative 1b as a good structure for further optimization in the search for antitubercular drugs.
Geopolymer-Based Artificial Aggregates: A Review on Methods of Producing, Properties, and Improving Techniques
Authors: Almadani Mohammad , Razak Rafiza Abd , Abdullah Mohd Mustafa Al Bakri , Mohamed Rosnita
Published: August 2022

The depletion of aggregate-related natural resources is the primary concern of all researchers globally. Recent studies emphasize the significance of recycling and reusing various types of natural or by-product material waste from industry as a result of the building industry’s rising demand for aggregate as the primary component in concrete production. It has been demonstrated that the geopolymer system has exceptional features, such as high strength, superior durability, and greater resistance to fire exposure, making it a viable alternative to ordinary Portland Cement (OPC) concrete. This study will examine the present method utilized to generate artificial aggregate-based geopolymers, including their physical and mechanical properties, as well as their characterization. The production process of geopolymer derived from synthetic aggregates will be highlighted. In conjunction with the bonding of aggregates and the cement matrix, the interfacial transition zone (ITZ) is highlighted in this work as an additional important property to be researched in the future. It will be discussed how to improve the properties of geopolymers based on artificial aggregates. It has been demonstrated that cold bonding provides superior qualities for artificial aggregate while conserving energy during production. The creation of ITZ has a significant impact on the bonding strength between artificial aggregates and the cement matrix. Additionally, improvement strategies demonstrate viable methods for enhancing the quality of manufactured aggregates. In addition, other recommendations are discussed in this study for future work.
An Overview of Laser Metal Deposition for Cladding: Defect Formation Mechanisms, Defect Suppression Methods and Performance Improvements of Laser-Cladded Layers
Authors: Cheng Jian , Xing Yunhao , Dong Enjie , Zhao Linjie , Liu Henan , Chang Tingyu , Chen Mingjun , Wang Jinghe , Lu Junwen , Wan Jun
Published: August 2022

With the development of society and the economy, there is an increasing demand for surface treatment techniques that can efficiently utilize metal materials to obtain good performances in the fields of mechanical engineering and the aerospace industry. The laser metal deposition (LMD) technique for cladding has become a research focus in recent years because of its lower dilution rate, small heat-effect zone and good metallurgical bonding between the coating and substrate. This paper reviews the simulation technology for the melt pool’s grain growth mechanism, temperature and stress distribution that are directly related to defect formation in LMD technology. At the same time, the defect suppression method and the performance improvement method of the cladded layer in LMD technology are introduced. Finally, it is pointed out that the active selection of materials according to the required performance, combined with the controllable processing technology, to form the corresponding microstructure, and finally, to actively realize the expected function, is the future development direction of LMD technology.
Cutting of Diamond Substrate Using Fixed Diamond Grain Saw Wire
Authors: Kamiya Osamu , Takahashi Mamoru , Miyano Yasuyuki , Ito Shinichi , Nakatsu Masanobu , Mizuma Hiroyuki , Iwama Yuichi , Murata Kenji , Nanao Junpei , Kawano Makoto , Maisawa Arata , Kazumi Takashi
Published: August 2022

This study demonstrates that a single-crystal diamond substrate can be cut along designed lines using the diamond-saw-wire cutting method. We developed an original saw-wire fixed diamond-grain using a bronze solder with a high melting temperature. We created a unique product machine system with a high vacuum furnace and a bronze solder that contains a metallic compound. The diamond cutting mechanism employed in this study is based on the mild wear phenomenon, owing to the friction between the diamond surfaces. A linear relationship between the cutting length and wire feed distance was observed. The relationship can be approximated as y = 0.3622x, where y (μm) is the cutting depth and x (km) is the wire feed distance. The life of the saw-wire was longer than that of the 6000 km wire feed distance and was tested by reciprocating an 8-m short wire at a speed, tension, and cutting force of 150 m/min, 1 N, and 0.2 N, respectively. A single crystal diamond substrate could be cut along the designed line, which was more than 2 mm long. The cutting speed was maintained constant at 0.36 μm/km.
Effects of Lithium Slag on the Frost Resistance of Cement-Soil
Authors: Chen Zhi , Chen Sili , Liu Liwen , Zhou Yuwan
Published: August 2022

In this study, the effect of lithium slag (LS) on the frost resistance of cement-soil was evaluated. The results of freeze–thaw damage on the surface of the cement-soil, freeze–thaw mass loss, unconfined compression strength, triaxial shear strength, cohesion, and internal friction angle were tested at various freeze–thaw cycles after 90 days of curing when LS was incorporated into the cement-soil at different proportions (0%, 6%, 12%, and 18%). Combining nuclear magnetic resonance (NMR) T2 distribution and scanning electron microscopy (SEM) microscopic images, the mechanism of the effect of LS on the cement-soil was also analyzed. The experiment confirmed that the surface freeze–thaw damage degree and mass loss value of the cement-soil decreased after incorporating different LS contents, and that the unconfined compression strength, triaxial shear strength, cohesion, and internal friction angle also improved significantly compared with the specimens without LS. In this experiment, the optimization level of the cement-soil performance with different LS content was ranked as 12% > 18% > 6% > 0%. According to the NMR and SEM analysis results, the LS content of 12% can optimize the internal pore structure of the cement-soil and strengthen the bond between aggregate particles, hence inhibiting the extension of freeze-swelling cracks induced by freeze–thaw cycles. In conclusion, LS can effectively enhance the frost resistance of cement-soil, and the optimum content in this experiment is 12%.
Green Facile Synthesis of Silver-Doped Zinc Oxide Nanoparticles and Evaluation of Their Effect on Drug Release
Authors: Khatir Nadia Mahmoudi , Sabbagh Farzaneh
Published: August 2022

Silver doped zinc oxide nanoparticles (ZANPs) were synthesized by the gelatin mediated and polymerized sol-gel method, and a calcination temperature of 700 °C was applied for 2 h. X-ray diffraction (XRD), FESEM, TGA, DSC, and EDS were performed to study the structure of the prepared nano-powders. Both cubic silver and hexagonal ZnO diffraction peaks were detected in the XRD patterns. The XRD results, analyzed by the size strain plot (SSP) and Scherrer methods, showed that the crystalline sizes of these nanoparticles increased as the Ag concentration increased. The results were observed via transition electron microscopy (TEM), where the particle size of the prepared samples was increased in the presence of silver. Catechin was chosen as a drug model and was loaded into the hydrogels for release studies. The drug content percentage of catechin in the hydrogels showed a high loading of the drug, and the highest rate was 98.59 ± 2.11%, which was attributed to the Zn0.97Ag0.03O hydrogels. The swelling of the samples and in vitro release studies were performed. The results showed that Zn0.91Ag0.09O showed the highest swelling ratio (68 ± 3.40%) and, consequently, the highest release (84 ± 2.18%) within 300 min. The higher amount of silver ions in the hydrogel structure causes it to enhance the osmotic pressure of the inner structure and increases the relaxation of the structure chain.
Mathematical Model of Suspended Particles Transport in the Estuary Area, Taking into Account the Aquatic Environment Movement
Authors: Sukhinov Alexander , Chistyakov Alexander , Kuznetsova Inna , Belova Yulia , Nikitina Alla
Published: August 2022

A large amount of contaminants enter marine systems with river runoff, so the purpose of the study is to model the transport of suspended particles in the estuary area. To describe hydrodynamic and hydrophysical processes, the mathematical model of the suspended particles transport was used, supplemented by a three-dimensional mathematical model of hydrodynamics, used to calculate the fields of the aquatic environment movement velocity vector, and equation for calculating the variable density. The approximation of the equations for calculating the velocity field by spatial variables is based on the splitting schemes for physical processes with fluid volume of the control areas, which allows for us to consider the complex geometry of the coastline and the bottom. The suspended particles transport model is approximated using splitting schemes for two-dimensional and one-dimensional problems. Numerical experiments were carried out to simulate the aquatic environment movement in the estuary area, the multicomponent suspension deposition, as well as mixing of waters in the mouth, taking into account the different density of the aquatic environment. The used models and methods allow to significantly improve the accuracy of modeling suspended particle transport and consider the factors influencing the studied processes.
Discriminative Convolutional Sparse Coding of ECG Signals for Automated Recognition of Cardiac Arrhythmias
Authors: Zhang Bing , Liu Jizhong
Published: August 2022

Electrocardiogram (ECG) is a common and powerful tool for studying heart function and diagnosing several abnormal arrhythmias. In this paper, we present a novel classification model that combines the discriminative convolutional sparse coding (DCSC) framework with the linear support vector machine (LSVM) classification strategy. In the training phase, most existing convolutional sparse coding frameworks are unsupervised in the sense that label information is ignored in the convolutional filter training stage. In this work, we explicitly incorporate a label consistency constraint called “discriminative sparse-code error” into the objective function to learn discriminative dictionary filters for sparse coding. The learned dictionary filters encourage signals from the same class to have similar sparse codes, and signals from different classes to have dissimilar sparse codes. To reduce the computational complexity, we propose to perform a max-pooling operation on the sparse coefficients. Using LSVM as a classifier, we examine the performance of the proposed classification system on the MIT-BIH arrhythmia database in accordance with the AAMI EC57 standard. The experimental results show that the proposed DCSC + LSVM algorithm can obtain 99.32% classification accuracy for cardiac arrhythmia recognition.
A Contribution of Liouville-Type Theorems to the Geometry in the Large of Hadamard Manifolds
Authors: Mikeš Josef , Rovenski Vladimir , Stepanov Sergey
Published: August 2022

A complete, simply connected Riemannian manifold of nonpositive sectional curvature is called a Hadamard manifold. In this article, we prove Liouville-type theorems for isometric and harmonic self-diffeomorphisms of Hadamard manifolds, as well as Liouville-type theorems for Killing–Yano, symmetric Killing and harmonic tensors on Hadamard manifolds.
Functionalization of Multi-Walled Carbon Nanotubes Changes Their Antibiofilm and Probiofilm Effects on Environmental Bacteria
Authors: Maksimova Yuliya , Bykova Yana , Maksimov Aleksandr
Published: August 2022

Releasing multi-walled carbon nanotubes (MWCNTs) into ecosystems affects the biofilm formation and metabolic activity of bacteria in aquatic and soil environments. Pristine (pMWCNTs), oleophilic (oMWCNTs), hydrophilic (hMWCNTs), and carboxylated (cMWCNTs) carbon nanotubes were used to investigate their effects on bacterial biofilm. A pronounced probiofilm effect of modified MWCNTs was observed on the Gram-negative bacteria of Pseudomonas fluorescens C2, Acinetobacter guillouiae 11 h, and Alcaligenes faecalis 2. None of the studied nanomaterials resulted in the complete inhibition of biofilm formation. The complete eradication of biofilms exposed to MWCNTs was not observed. The functionalization of carbon nanotubes was shown to change their probiofilm and antibiofilm effects. Gram-negative bacteria were the most susceptible to destruction, and among the modified MWCNTs, oMWCNTs had the greatest effect on biofilm destruction. The number of living cells in the biofilms was assessed by the reduction of XTT, and metabolic activity was assessed by the reduction of resazurin to fluorescent resorufin. The biofilms formed in the presence of MWCNTs reduced tetrozolium to formazan more actively than the control biofilms. When mature biofilms were exposed to MWCNTs, dehydrogenase activity decreased in Rhodococcus erythropolis 4-1, A. guillouiae 11 h, and A. faecalis 2 in the presence of pMWCNTs and hMWCNTs, as well as in A. guillouiae 11 h exposed to cMWCNTs. When mature biofilms were exposed to pMWCNTs, hMWCNTs, and cMWCNTs, the metabolism of cells decreased in most strains, and oMWCNTs did not have a pronounced inhibitory effect. The antibiofilm and probiofilm effects of MWCNTs were strain-dependent.