bluuebunny/crossref_metadata_2025 huggingface dataset:
Anti-Huntington’s Effect of Rosiridin via Oxidative Stress/AchE Inhibition and Modulation of Succinate Dehydrogenase, Nitrite, and BDNF Levels against 3-Nitropropionic Acid in Rodents
Authors: Afzal Muhammad , Sayyed Nadeem , Alharbi Khalid Saad , Alzarea Sami I. , Alshammari Mohammed Salem , Alomar Fadhel A. , Alenezi Sattam Khulaif , Quazi Anwarulabedin Mohsin , Alzarea Abdulaziz I. , Kazmi Imran
Published: July 2022

Background: Rosiridin is a compound extracted from Rhodiola sachalinensis; water extracts of Rhodiola root elicit positive effects on the human central nervous system and improve brain function. They are also thought to be beneficial to one’s health, in addition to being antioxidants. The present study aims to evaluate the anti-Huntington’s effect of rosiridin against 3-nitropropionic acid (3-NPA)-induced Huntington’s disease (HD)-like effects in rats. Materials and Methods: The acute toxicity in rats was elucidated to track the conceivable toxicities in the rats. The effectiveness of rosiridin at a dosage of 10 mg/kg was evaluated against several dose administrations of 3-NPA-induced HD-like symptoms in the rats for 22 days. At the end of the study, behavioral parameters were assessed as a hallmark for the cognitive and motor functions in the rats. Similarly, after the behavioral assessment, the animals were sacrificed to obtain a brain tissue homogenate. The prepared homogenate was utilized for the estimation of several biochemical parameters, including oxidative stress (glutathione, catalase, and malondialdehyde), brain-derived neurotrophic factor and succinate dehydrogenase activity, and the glutamate and acetylcholinesterase levels in the brain. Furthermore, inflammatory mediators linked to the occurrence of neuroinflammation in rats were evaluated in the perfused brain tissues. Results: The rosiridin-treated group exhibited a significant restoration of behavioral parameters, including in the beam-walk test, latency in falling during the hanging wire test, and percentage of memory retention during the elevated plus-maze test. Further, rosiridin modulated several biochemical parameters, including oxidative stress, pro-inflammatory activity, brain-derived neurotrophic factor, nitrite, and acetylcholinesterase as compared to disease control group that was treated with 3-NPA. Conclusions: The current study exhibits the anti-Huntington’s effects of rosiridin in experimental animal models.
CCL4 Functions as a Biomarker of Type 2 Airway Inflammation
Authors: Kobayashi Yoshiki , Chu Hanh Hong , Kanda Akira , Yun Yasutaka , Shimono Masami , Nguyen Linh Manh , Mitani Akitoshi , Suzuki Kensuke , Asako Mikiya , Iwai Hiroshi
Published: July 2022

Eosinophilic airway inflammatory disease is associated with bronchial asthma, with eosinophilic chronic rhinosinusitis (ECRS) typical of refractory type 2 airway inflammation. CCL4 produced at local inflammatory sites is involved in them via the accumulation and activation of type 2 inflammatory cells, including eosinophils. The detailed mechanism of CCL4 production remains unclear, and also the possibility it could function as a biomarker of type 2 airway inflammation remains unresolved. In this study, we evaluated CCL4 mRNA expression and production via the TSLP receptor (TSLPR) and toll-like receptors (TLRs) or proteinase-activated receptor-2 (PAR2) in BEAS-2B bronchial epithelial cells co-incubated with purified eosinophils or eosinophil peroxidase (EPX). We examined serum chemokine (CCL4, CCL11, CCL26, and CCL17) and total IgE serum levels, fractionated exhaled nitrogen oxide (FENO), and CCL4 expression in nasal polyps in patients with severe ECRS and asthma. CCL4 was induced by TSLP under eosinophilic inflammation. Furthermore, CCL4 was released via TLR3 signaling, which was enhanced by TSLP. CCL4 was mainly located in nasal polyp epithelial cells, while serum CCL4 levels were reduced after dupilumab treatment. Serum CCL4 levels were positively correlated with FENO, serum IgE, and CCL17 levels. Thus, CCL4 released from epithelial cells via the innate immune system during type 2 airway inflammation may function as a useful biomarker for the condition.
Capsanthin Inhibits Atherosclerotic Plaque Formation and Vascular Inflammation in ApoE−/− Mice
Authors: Kim Sungmin , Lee Yu-Ran , Lee Eun-Ok , Jin Hao , Choi Yeon-Hee , Joo Hee-Kyoung , Jeon Byeong-Hwa
Published: July 2022

Capsanthin is a red pigment and the major carotenoid component of red paprika (Capsicum annuum L.). However, its role in atherosclerosis is yet to be fully elucidated. This study investigated the role of dietary capsanthin in vascular inflammation in atherosclerotic mice. We evaluated the anti-atherosclerotic effects of daily oral administration of capsanthin (0.5 mg/kg of body weight/day) in apolipoprotein E-deficient (ApoE−/−) mice fed a Western-type diet (WD). Capsanthin treatment inhibited vascular cell adhesion molecule 1 expression and nuclear factor-κB ser536 phosphorylation in tumor necrosis factor-α-stimulated cultured endothelial cells. Dietary capsanthin significantly inhibited the WD-induced elevation in the plasma levels of total cholesterol, low-density lipoprotein cholesterol (LDL-C), and triglyceride in mice. Interestingly, capsanthin reduced aortic plaque formation and VCAM-1 expression, which is vascular inflammation, in atherosclerotic mice. In addition, the neutrophil–lymphocyte ratio, a systemic inflammatory marker, was inhibited in capsanthin-treated mice. Furthermore, capsanthin significantly reduced the levels of proinflammatory cytokines, such as TNF-α, interleukin-6, and monocyte chemoattractant protein-1, in the plasma of atherosclerotic mice. Collectively, our data demonstrate that dietary capsanthin plays a protective role against atherosclerosis in hyperlipidemic mice. This protective effect could be attributed to the anti-inflammatory properties of capsanthin.
Systems Genetic Identification of Mitochondrion-Associated Alzheimer’s Disease Genes and Implications for Disease Risk Prediction
Authors: Xu Xuan , Wang Hui , Bennett David A. , Zhang Qing-Ye , Wang Gang , Zhang Hong-Yu
Published: July 2022

Cumulative evidence has revealed the association between mitochondrial dysfunction and Alzheimer’s disease (AD). Because the number of mitochondrial genes is very limited, the mitochondrial pathogenesis of AD must involve certain nuclear genes. In this study, we employed systems genetic methods to identify mitochondrion-associated nuclear genes that may participate in the pathogenesis of AD. First, we performed a mitochondrial genome-wide association study (MiWAS, n = 809) to identify mitochondrial single-nucleotide polymorphisms (MT-SNPs) associated with AD. Then, epistasis analysis was performed to examine interacting SNPs between the mitochondrial and nuclear genomes. Weighted co-expression network analysis (WGCNA) was applied to transcriptomic data from the same sample (n = 743) to identify AD-related gene modules, which were further enriched by mitochondrion-associated genes. Using hub genes derived from these modules, random forest models were constructed to predict AD risk in four independent datasets (n = 743, n = 542, n = 161, and n = 540). In total, 9 potentially significant MT-SNPs and 14,340 nominally significant MT-nuclear interactive SNPs were identified for AD, which were validated by functional analysis. A total of 6 mitochondrion-related modules involved in AD pathogenesis were found by WGCNA, from which 91 hub genes were screened and used to build AD risk prediction models. For the four independent datasets, these models perform better than those derived from AD genes identified by genome-wide association studies (GWASs) or differential expression analysis (DeLong’s test, p  <  0.05). Overall, through systems genetics analyses, mitochondrion-associated SNPs/genes with potential roles in AD pathogenesis were identified and preliminarily validated, illustrating the power of mitochondrial genetics in AD pathogenesis elucidation and risk prediction.
Niosome-Assisted Delivery of DNA Fluorescent Probe with Optimized Strand Displacement for Intracellular MicroRNA21 Imaging
Authors: Zhu Zongwei , Zhang Hongqian , Dong Xiaoxue , Lin Meng , Yang Chuanxu
Published: July 2022

MicroRNAs play a vital role in cancer development and are considered as potential biomarkers for early prognostic assessment. Here, we propose a novel biosensing system to achieve fluorescence imaging of miRNA21 (miR21) in cancer cells. This system consists of two components: an optimized “off-on” double-stranded DNA (dsDNA) fluorescent for miR21 sensing by efficient strand-displacement reaction and a potent carrier vesicle, termed niosome (SPN), to facilitate the efficient intracellular delivery of the dsDNA probe. A series of dsDNA probes based on fluorescence energy resonance transfer (FRET) was assembled to target miR21. By optimizing the appropriate length of the reporter strand in the dsDNA probe, high accuracy and sensitivity for miR21 recognition are ensured. To overcome the cellular barrier, we synthesized SPN with the main components of a nonionic surfactant Span 80 and a cationic lipid DOTAP, which could efficiently load dsDNA probes via electrostatic interactions and potently deliver the dsDNA probes into cells with good biosafety. The SPN/dsDNA achieved efficient miR21 fluorescent imaging in living cells, and could discriminate cancer cells (MCF-7) from normal cells (L-02). Therefore, the proposed SPN/dsDNA system provides a powerful tool for intracellular miRNA biosensing, which holds great promise for early cancer diagnosis.
Production and Selection of Antibody–Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues
Authors: Wu Yuxiang , Fan Qi , Chen Yinuo , Sun Xia , Shi Guoqing
Published: July 2022

To produce a sensitive monoclonal antibody (mAb) for the simultaneous detection of carbofuran, benfuracarb, carbosulfan and 3-hydroxy-carbofuran, 2,3-dihydro-2,2-dimethyl-7-benzofuranmethanamine (DDB) was conjugated to bovine serum albumin (BSA) to prepare the immunogen DDB-BSA and mice were immunized. Coating antigens were prepared by conjugating DDB and 5-methoxy-2,3-dihydrobenzofuran-3-acetic acid (MDA) to BSA and ovalbumin (OVA), respectively. Furthermore, the effect of different antibody–antigen pairs on the sensitivity of ELISA and LFIA methods for the detection of carbofuran was investigated. After the immunization, a high-affinity mAb 13C8 was obtained. The ability of the coating antigen to compete with carbofuran for binding antibodies was found to be significantly different between ELISA and LFIA methods. With the antibody–antigen pair 13C8-MDA-OVA, the IC50 values of the ELISA and QD-LFIA methods for carbofuran were 0.18 ng/mL and 0.67 ng/mL, respectively. The cross-reactivity (CR) values of the two methods for benfuracarb, carbosulfan and 3-hydroxy-carbofuran ranged from 72.0% to 83.7%, while, for other carbamate pesticides, the CR values were less than 1%. The spiked recoveries of carbofuran in vegetables by the QD-LFIA method were 83–111%, with a coefficient of variation below 10%, and the test results of the actual samples were consistent with the HPLC-MS method. Overall, this study provides key materials for the development of immunoassays for carbofuran and its analogues, and the antibody–antigen pair selection strategy established in this study provides useful insights for the development of sensitive immunoassays for other compounds.
3D Space Layout Design of Holographic Command Cabin Information Display in Mixed Reality Environment Based on HoloLens 2
Authors: Wang Wei , Hong Xuefeng , Dang Sina , Xu Ning , Qu Jue
Published: July 2022

After the command and control information of the command and control cabin is displayed in the form of mixed reality, the large amount of real-time information and static information contained in it will form a dynamic situation that changes all the time. This brings a great burden to the system operator’s cognition, decision-making and operation. In order to solve this problem, this paper studies the three-dimensional spatial layout of holographic command cabin information display in a mixed reality environment. A total of 15 people participated in the experiment, of which 10 were the subjects of the experiment and 5 were the staff of the auxiliary experiment. Ten subjects used the HoloLens 2 generation to conduct visual characteristics and cognitive load experiments and collected and analyzed the subjects’ task completion time, error rate, eye movement and EEG and subjective evaluation data. Through the analysis of experimental data, the laws of visual and cognitive features of three-dimensional space in a mixed reality environment can be obtained. This paper systematically explores the effects of three key attributes: depth distance, information layer number and target relative position depth distance of information distribution in a 3D space, on visual search performance and on cognitive load. The experimental results showed that the optimal depth distance range for information display in the mixed reality environment is: the best depth distance for operation interactions (0.6 m~1.0 m), the best depth distance for accurate identification (2.4 m~2.8 m) and the overall situational awareness best-in-class depth distance (3.4 m~3.6 m). Under a certain angle of view, the number of information layers in the space is as small as possible, and the number of information layers should not exceed five at most. The relative position depth distance between the information layers in space ranges from 0.2 m to 0.35 m. Based on this theory, information layout in a 3D space can achieve a faster and more accurate visual search in a mixed reality environment and effectively reduce the cognitive load.
Vitamin D Deficiency and Risk Factors Related to Acute Psychiatric Relapses in Patients with Severe Mental Disorders: A Preliminary Study
Authors: Fabrazzo Michele , Agnese Salvatore , Cipolla Salvatore , Di Vincenzo Matteo , Mancuso Emiliana , Volpicelli Antonio , Perris Francesco , Sampogna Gaia , Catapano Francesco , Fiorillo Andrea , Luciano Mario
Published: July 2022

Previous studies have indicated that vitamin (Vit) D deficiency is frequent in psychiatric patients, regardless of diagnostic category. We aimed to assess whether acute psychiatric relapses in inpatients was associated with Vit D deficiency compared to stabilized outpatients. The cohort (152 total patients, 75 males and 77 females) had a mean age of 47.3 ± 14.4 years at admission and was grouped according to psychiatric diagnosis. Psychopathological symptom severity was assessed by the Brief Psychiatric Rating Scale (BPRS), a multidimensional symptom inventory. Total calcium serum levels were measured using standard laboratory methods, while plasma levels of 25-OH-Vit D and parathyroid hormone (PTH) were measured by automated chemiluminescence immunoassays. The psychiatric inpatient subgroup showed a significant difference in serum levels of 25-OH-Vit D and PTH (p < 0.001). Correlation analysis between serum levels of 25-OH-Vit D and BPRS total and subitem scores indicated a significantly negative relationship. In addition, linear regression analysis evidenced that the inpatient condition might predict low PTH and 25-OH-Vit D serum levels. Hospitalized psychiatric patients are at increased risk for Vit D deficiency regardless of their diagnostic categories. The mechanism underlying the association between acute psychiatric relapses and Vit D deficiency remains unclear. Therefore, screening for Vit D deficiency should pertain to the health assessment of patients with major psychiatric disorders.
Patients with Methamphetamine Use Disorder Show Highly Utilized Proactive Inhibitory Control and Intact Reactive Inhibitory Control with Long-Term Abstinence
Authors: Dai Weine , Zhou Hui , Møller Arne , Wei Ping , Hu Kesong , Feng Kezhuang , Han Jie , Li Qi , Liu Xun
Published: July 2022

Methamphetamine use disorder (MUD) is a chronic brain disorder that involves frequent failures of inhibitory control and relapses into methamphetamine intake. However, it remains unclear whether the impairment of inhibitory control in MUD is proactive, reactive or both. To address this issue, the current study used the conditional stop-signal task to assess proactive and reactive inhibitory control in 35 MUD patients with long-term abstinence and 35 matched healthy controls. The results showed that MUD patients with long-term abstinence had greater preparation costs than healthy controls, but did not differ in performance, implying a less efficient utilization of proactive inhibitory control. In contrast, MUD patients exhibited intact reactive inhibitory control; reactive but not proactive inhibitory control was associated with high sensation seeking in MUD patients with long-term abstinence. These findings suggest that proactive and reactive inhibitory control may be two different important endophenotypes of addiction in MUD patients with long-term abstinence. The current study provides new insight into the uses of proactive and reactive inhibitory control to effectively evaluate and precisely treat MUD patients with long-term abstinence.
An Investigation of the Neurotoxic Effects of Malathion, Chlorpyrifos, and Paraquat to Different Brain Regions
Authors: Elmorsy Ekramy , Al-Ghafari Ayat , Al Doghaither Huda , Salama Mohamed , Carter Wayne G.
Published: July 2022

Acute or chronic exposures to pesticides have been linked to neurotoxicity and the potential development of neurodegenerative diseases (NDDs). This study aimed to consider the neurotoxicity of three widely utilized pesticides: malathion, chlorpyrifos, and paraquat within the hippocampus (HC), corpus striatum (CS), cerebellum (CER), and cerebral cortex (CC). Neurotoxicity was evaluated at relatively low, medium, and high pesticide dosages. All pesticides inhibited acetylcholinesterase (AChE) and neuropathy target esterase (NTE) in each of the brain regions, but esterase inhibition was greatest in the HC and CS. Each of the pesticides also induced greater disruption to cellular bioenergetics within the HC and CS, and this was monitored via inhibition of mitochondrial complex enzymes I and II, reduced ATP levels, and increased lactate production. Similarly, the HC and CS were more vulnerable to redox stress, with greater inhibition of the antioxidant enzymes catalase and superoxide dismutase and increased lipid peroxidation. All pesticides induced the production of nuclear Nrf2 in a dose-dependent manner. Collectively, these results show that pesticides disrupt cellular bioenergetics and that the HC and CS are more susceptible to pesticide effects than the CER and CC.
An Analytical Model for the Impact of Building Control and Automation Upgrade on Space Heating Energy Efficiency
Authors: Morkunaite Lina , Pupeikis Darius , Jurelionis Andrius , Fokaides Paris A. , Papadopoulos Agis
Published: July 2022

Intelligent building management systems are proven to lead to energy savings and are an integral component of smart buildings. The procedures developed in the EN standards describe the methodology for calculating the energy savings achieved by improving the automation and control levels of separate services in building systems. However, although this method is used in practice, it is rarely applied or investigated by the research community. Typically, energy savings resulting from a single automation improvement intervention in a building heating system are observed, while the holistic view of combined automation upgrades is not considered. Therefore, the purpose of this study was to assess the energy savings resulting from several upgrades to control levels in the heating system components of the building. In addition, this research provides a rationale for the impact of multiple automation and control options for heating systems as well as examines the difference in energy savings. Finally, an analytical model is developed and demonstrated to assess the feasibility of building automation and control upgrades, by determining the allowed investment according to a set of predefined indicators.
NIOSH Dampness and Mold Assessment Tool (DMAT): Documentation and Data Analysis of Dampness and Mold-Related Damage in Buildings and Its Application
Authors: Park Ju-Hyeong , Cox-Ganser Jean
Published: July 2022

Indoor dampness and mold are prevalent, and the exposure has been associated with various illnesses such as the exacerbation of existing asthma, asthma development, current asthma, ever-diagnosed asthma, bronchitis, respiratory infection, allergic rhinitis, dyspnea, wheezing, cough, upper respiratory symptoms, and eczema. However, assessing exposures or environments in damp and moldy buildings/rooms, especially by collecting and analyzing environmental samples for microbial agents, is complicated. Nonetheless, observational assessment (visual and olfactory inspection) has been demonstrated as an effective method for evaluating indoor dampness and mold. The National Institute for Occupational Safety and Health developed an observational assessment method called the Dampness and Mold Assessment Tool (DMAT). The DMAT uses a semi-quantitative approach to score the level of dampness and mold-related damage (mold odor, water damage/stains, visible mold, and wetness/dampness) by intensity or size for each of the room components (ceiling, walls, windows, floor, furnishings, ventilation system, pipes, and supplies and materials). Total or average room scores and factor-or component-specific scores can be calculated for data analysis. Because the DMAT uses a semi-quantitative scoring method, it better differentiates the level of damage compared to the binary (presence or absence of damage) approach. Thus, our DMAT provides useful information on identifying dampness and mold, tracking and comparing past and present damage by the scores, and prioritizing remediation to avoid potential adverse health effects in occupants. This protocol-type article describes the DMAT and demonstrates how to apply it to effectively manage indoor dampness and mold-related damage.
Influence of Non-Constant Hygrothermal Parameters on Heat and Moisture Transfer in Rammed Earth Walls
Authors: Tan Jiaye , Liang Jiahua , Wan Li , Jiang Bin
Published: July 2022

As environment-friendly building materials, earth materials are attracting significant attention because of their favorable hygrothermal properties. In this study, the earth materials in northwest Sichuan were tested and curves of thermal conductivity and water vapor permeability with relative humidity were obtained. The function curves and constants of the two coefficients were substituted into the verified nonstationary model of heat and moisture transfer in rammed earth walls and indoor air for calculation. The difference in the calculation results when the hygrothermal parameters are functions and constants were analyzed, and the influence of the non-constant hygrothermal parameters on the heat and moisture transfer in rammed earth walls, was obtained. The test results show that thermal conductivity is linearly related to moisture content, and water vapor permeability has a small variation in the relative humidity range of 0–60% and increases exponentially above 60%. The calculation results indicate that the non-constant hygrothermal parameters have little influence on the internal surface temperature of the rammed earth walls and Mianyang City’s indoor air temperature and humidity during the summer and winter. The heat transfer on the internal surface will be underestimated by using a non-constant for the hygrothermal parameter when the moisture content of the wall is low, and vice versa. In hot-humid areas or seasons with large differences in temperature and humidity between indoors and outdoors, non-constant hygrothermal parameters have a more obvious effect on heat transfer on the internal surface of the wall. The results of this study demonstrate the necessity of parameter testing.
Behavior Choice Mechanisms and Tax Incentive Mechanisms in the Game of Construction Safety
Authors: Liu Jun , Wang Xinhua , Liu Tao
Published: July 2022

The violation behavior of construction workers is an important cause of construction accidents. To reduce the violations of construction workers and to stimulate the supervision behavior of local governments and construction enterprises, an evolutionary game model is constructed in this paper. Then, the behavior choice mechanism of each player is analyzed. Finally, an incentive effect analysis method is put forward, and the incentive effects of different tax incentive mechanisms are analyzed. This research finds that only when the safety punishment imposed on construction workers is large enough does the supervision behavior of local governments and construction enterprises encourage construction workers to choose not to violate the regulation. Increasing the tax rate of a construction enterprise in the case of accidents can encourage the construction enterprise to supervise, but it inhibits the supervision behavior of the local government. A numerical simulation verifies the effectiveness of the incentive effect analysis method, which provides a new method for the incentive effect analysis of incentive mechanisms.
A Foundation Model for Building Digital Twins: A Case Study of a Chiller
Authors: Li Suliang , Yang Qiliang , Xing Jianchun , Chen Wenjie , Zou Rongwei
Published: July 2022

Due to the high-fidelity mapping of the physical buildings and the intelligent performance shown in their lifecycle, digital twins (DTs) have gained increasing attention in the building sector. Although digital twins based on building information modeling (BIM) have become a hot research topic, existing works emphasize the digitization of building static and dynamic information and lack a unified consideration of the inherent physical mechanisms and interactive behaviors of buildings. To this end, this paper proposes a foundation model for building digital twins which realizes the unification of building static information, physical mechanisms and interaction patterns. The conceptual framework of the model is given first and then formal modeling and verification with time automata theory are performed to demonstrate the plausibility of the model. Finally, a practical digital twin of a chiller is developed based on the proposed foundation model as an example, thus, indicating its effectiveness and credibility.
Identifying Critical Influencing Factors of the Value Creation of Urban Rail Transit PPP Projects in China
Authors: Li Xuewei , Yuan Jingfeng , Liu Xuan , Ke Yongjian , Jia Sijia
Published: July 2022

Value creation is the primary motivation for public-private cooperation. The development of urban rail transit (URT) projects requires heavy investment and abundant construction and management experience. Thus, public-private partnership (PPP) is usually adopted in URT project development to benefit the public and private sectors. However, with the high-quality development of PPP projects, the value creation of URT PPP projects is not only about achieving basic project objectives but also relying on partner synergy to achieve value-added. Based on the extended connotation of value creation, this study intends to systematically identify the influencing factors of value creation of URT PPP projects in China. The grounded theory approach was adopted to deduce the influencing factors of value creation through analyzing the various types of articles related to Chinese URT PPP projects. Resources complementarity among stakeholders, cooperation environment, and partnership synergy were identified as the main influencing factors. Meanwhile, a theoretical model that described how these influencing factors combined to promote value creation during public-private sectors cooperation of URT projects was established. This research helps broaden the understanding of how public and private sectors should cooperate and collaborate in URT projects to realize value maximization and promote the sustainable development of URT PPP projects and provides a model for further empirical examination.
A New Approach to Automatically Calibrate and Detect Building Cracks
Authors: Liu Zongchao , Li Xiaoda , Li Junhui , Teng Shuai
Published: July 2022

Timely crack detection plays an important role in building damage assessment. In this study, an automatic crack detection method based on image registration and pixel-level segmentation (improved DeepLab_v3+) is proposed. Firstly, the moving images are calibrated by image registration, and the similarity method is adopted to evaluate the calibrated results. Secondly, the DeepLab_v3+ is improved and used to segment the fixed images and the calibrated images. Finally, the difference of crack pixels between the fixed and calibrated images is estimated, and the key parameter is investigated to find the optimal optimizer and learning rate. The results illustrate that: (1) the image registration technology shows excellent calibration achievement and the average error is only 4%; (2) with the resnet50 being selected as the backbone network of improved Deeplab_v3+, the automatic detection method proposed in this study is more efficient in comparison with other common pixel-level segmentation algorithms; (3) the best network optimizer of improved Deeplab_v3+ and learning rate of crack segmentation task are sgdm and 0.001, respectively. The crack detection method proposed in this study can significantly improves the technical level of crack detection in practical projects.
Repositioning of Old Drugs for Novel Cancer Therapies: Continuous Therapeutic Perfusion of Aspirin and Oseltamivir Phosphate with Gemcitabine Treatment Disables Tumor Progression, Chemoresistance, and Metastases
Authors: Qorri Bessi , Mokhtari Reza Bayat , Harless William W. , Szewczuk Myron R.
Published: July 2022

Metastatic pancreatic cancer has an invariably fatal outcome, with an estimated median progression-free survival of approximately six months employing our best combination chemotherapeutic regimens. Once drug resistance develops, manifested by increased primary tumor size and new and growing metastases, patients often die rapidly from their disease. Emerging evidence indicates that chemotherapy may contribute to the development of drug resistance through the upregulation of epithelial–mesenchymal transition (EMT) pathways and subsequent cancer stem cell (CSC) enrichment. Neuraminidase-1 (Neu-1) regulates the activation of several receptor tyrosine kinases implicated in EMT induction, angiogenesis, and cellular proliferation. Here, continuous therapeutic targeting of Neu-1 using parenteral perfusion of oseltamivir phosphate (OP) and aspirin (ASA) with gemcitabine (GEM) treatment significantly disrupts tumor progression, critical compensatory signaling mechanisms, EMT program, CSC, and metastases in a preclinical mouse model of human pancreatic cancer. ASA- and OP-treated xenotumors significantly inhibited the metastatic potential when transferred into animals.
HDAC2- and EZH2-Mediated Histone Modifications Induce PDK1 Expression through miR-148a Downregulation in Breast Cancer Progression and Adriamycin Resistance
Authors: Xie Yunxia , Shi Zhumei , Qian Yingchen , Jiang Chengfei , Liu Wenjing , Liu Bingjie , Jiang Binghua
Published: July 2022

Background: Breast cancer has one of highest morbidity and mortality rates for women. Abnormalities regarding epigenetics modification and pyruvate dehydrogenase kinase 1 (PDK1)-induced unusual metabolism contribute to breast cancer progression and chemotherapy resistance. However, the role and mechanism of epigenetic change in regulating PDK1 in breast cancer remains to be elucidated. Methods: Gene set enrichment analysis (GSEA) and Pearson’s correlation analysis were performed to analyze the relationship between histone deacetylase 2 (HDAC2), enhancer of zeste homologue 2 (EZH2), and PDK1 in database and human breast cancer tissues. Dual luciferase reporters were used to test the regulation between PDK1 and miR-148a. HDAC2 and EZH2 were found to regulate miR-148a expression through Western blotting assays, qRT-PCR and co-immunoprecipitation assays. The effects of PDK1 and miR-148a in breast cancer were investigated by immunofluorescence (IF) assay, Transwell assay and flow cytometry assay. The roles of miR-148a/PDK1 in tumor growth were investigated in vivo. Results: We found that PDK1 expression was upregulated by epigenetic alterations mediated by HDAC2 and EZH2. At the post-transcriptional level, PDK1 was a new direct target of miR-148a and was upregulated in breast cancer cells due to miR-148a suppression. PDK1 overexpression partly reversed the biological function of miR-148a—including miR-148a’s ability to increase cell sensitivity to Adriamycin (ADR) treatment—inhibiting cell glycolysis, invasion and epithelial–mesenchymal transition (EMT), and inducing apoptosis and repressing tumor growth. Furthermore, we identified a novel mechanism: DNMT1 directly bound to EZH2 and recruited EZH2 and HDAC2 complexes to the promoter region of miR-148a, leading to miR-148a downregulation. In breast cancer tissues, HDAC2 and EZH2 protein expression levels also were inversely correlated with levels of miR-148a expression. Conclusion: Our study found a new regulatory mechanism in which EZH2 and HDAC2 mediate PDK1 upregulation by silencing miR-148a expression to regulate cancer development and Adriamycin resistance. These new findings suggest that the HDAC2/EZH2/miR-148a/PDK1 axis is a novel mechanism for regulating cancer development and is a potentially promising target for therapeutic options in the future.
Prognostic Impact of Neutropenia in Cancer Patients with Septic Shock: A 2009–2017 Nationwide Cohort Study
Authors: Kim Sang-Min , Kim Youn-Jung , Kim Ye-Jee , Kim Won-Young
Published: July 2022

(1) Background: Neutropenia’s prognostic impact on mortality in cancer patients with septic shock remains controversial despite recent advances in cancer and sepsis management. This population-based, case–control study aimed to determine whether neutropenia could be related to an increase in short-term and long-term mortality. (2) Methods: This population-based, case–control study used data from the National Health Insurance Service of Korea. Adult cancer patients who presented to the emergency department with septic shock from 2009 to 2017 were included. The 30-day and 1-year mortality rates were evaluated as short-term and long-term outcomes. Cox proportional hazard regression was performed after adjusting for age, sex, Charlson comorbidity index, and neutropenia. (3) Results: In 43,466 adult cancer patients with septic shock, the 30-day and 1-year mortality rates were 52.1% and 81.3%, respectively. In total, 6391 patients had neutropenic septic shock, and the prevalent cancer type was lung cancer, followed by leukemia, non-Hodgkin’s lymphoma, stomach cancer, and colon cancer. Furthermore, 30-day and 1-year mortality was lower in patients with neutropenia than in those without neutropenia. After adjustment for confounders, neutropenia was independently associated with decreased 30-day and 1-year mortality rates. (4) Conclusions: In cancer patients presenting to the emergency department with septic shock, the presence of neutropenia did not increase mortality. This suggests that neutropenia may not be used as a single triage criterion for withholding intensive care in cancer patients presenting to the emergency department with septic shock.
Impact of Statin Use on Localized Prostate Cancer Outcomes after Radiation Therapy: Long-Term Follow-Up
Authors: Kaulanjan Kevin , Lavigne Danny , Saad Fred , Karakiewicz Pierre I. , Flammia Rocco Simone , Kluth Luis Alex , Mandel Philipp , Chun Felix K. -H. , Taussky Daniel , Hoeh Benedikt
Published: July 2022

The impact of statin use on localized prostate cancer (PCa) remains controversial, especially for patients treated with radiation therapy. We assessed the impact of statin use on biochemical recurrence (BCR) in patients treated for PCa with different modalities of radiation therapy. We evaluated 3555 patients undergoing radiation therapy between January 2001 and January 2022. The impact of statin use on BCR was analyzed for three treatment groups: external beam radiotherapy (EBRT), low-dose-rate seed brachytherapy (LDR), and EBRT plus high-dose-rate brachytherapy (EBRT + HDR). Median follow-up was 52 months among 1208 patients treated with EBRT, 1679 patients treated with LDR, and 599 patients treated with EBRT + HDR. A total of 1544 (43%) patients were taking a statin at the time of treatment, and 497 (14%) patients were in the D’Amico high-risk group. Only intermediate-risk patients treated with LDR fared better with statin use in univariate analysis (p = 0.025). This association was not significant in multivariate analysis (HR 0.44, 95% CI 0.18–1.10, p = 0.06). Statin use was not associated with a reduced risk of BCR in patients treated with radiation therapy. In the era of precision medicine, further investigation is needed to assess the benefit of statins in well-defined patients.
2D Nanomaterial—Based Electrocatalyst for Water Soluble Hydroperoxide Reduction
Authors: Pimpilova Mariya , Ivanova-Kolcheva Vanina , Stoyanova Maria , Dimcheva Nina
Published: July 2022

Hydroperoxides generated on lipid peroxidation are highly reactive compounds, tend to form free radicals, and their elevated levels indicate the deterioration of lipid samples. A good alternative to the classical methods for hydroperoxide monitoring are the electroanalytical methods (e.g., a catalytic electrode for their redox-transformation). For this purpose, a series of metal oxides—doped graphitic carbon nitride 2D nanomaterials—have been examined under mild conditions (pH = 7, room temperature) as catalysts for the electrochemical reduction of two water-soluble hydroperoxides: hydrogen peroxide and tert-butyl hydroperoxide. Composition of the electrode modifying phase has been optimized with respect to the catalyst load and binding polymer concentration. The resulting catalytic electrode has been characterized by impedance studies, cyclic voltammetry and chronoamperometry. Electrocatalytic effect of the Co-g-C3N4/Nafion modified electrode on the electrochemical reduction of both hydroperoxides has been proved by comparative studies. An optimal range of operating potentials from −0.215 V to −0.415 V (vs. RHE) was selected with the highest sensitivity achieved at −0.415 V (vs. RHE). At this operating potential, a linear dynamic range from 0.4 to 14 mM has been established by means of constant-potential chronoamperometry with a sensitivity, which is two orders of magnitude higher than that obtained with polymer-covered electrode.
Effects of Flue Gas Impurities on the Performance of Rare Earth Denitration Catalysts
Authors: Bian Xue , Lv Kaikai , Cai Ming , Cen Peng , Wu Wenyuan
Published: July 2022

Selective catalytic reduction (SCR) is still the most widely used process for controlling NOx gas pollution. Specifically, commercial vanadium-based catalysts have problems such as narrow operating temperature range and environmental pollution. Researchers have developed a series of cerium-based catalysts with good oxygen storage performance and excellent redox performance of CeO2. However, the anti-poisoning performance of the catalyst is the key to its application. There are many kinds of impurities in the flue gas, which has a huge impact on the catalyst. The deposition of substances, the reduction of active sites, the reduction of specific surface area, and the reduction of chemically adsorbed oxygen will affect the denitration activity of the catalyst to varying degrees, and the poisoning mechanism of different impurities on the catalyst is also different. Therefore, this review divides the impurities contained in flue gas into different types such as alkali metals, alkaline earth metals, heavy metals, and non-metals, and summarizes the effects and deactivation mechanisms of various types of impurities on the activity of rare earth catalysts. Finally, we hope that this work can provide a valuable reference for the development and application of NH3-SCR catalysts for rare earth denitration in the field of NOx control.
F−@d4r, a New Type of Acidic Catalytic Site in Zeolite
Authors: Zhao Yukai , Liang Weichi , Wang Yihan , Tong Yan , Li Zhanhong , Liang Yuqian , Liu Xiaolong , Jiang Jiuxing
Published: July 2022

As a solid acid, zeolite has been widely used in many fields such as tail gas treatment, petrochemical engineering, and the fine chemical industry. F− has been widely used in the synthesis of pure silica or high silica zeolites. To balance the charge of organic structure directing agents (OSDA), F− is often found located at the center of the double-4-rings (d4r) of the as-made zeolites. During calcination, fluorine ion is removed with the OSDA. We screened a series of composition building units and found that d4r is capable to retain F− in zeolite structure. We introduce the F− back after the calcination to create an unprecedented type of acid site, i.e., F−@d4r in pure silica zeolites ITQ-12. The F−@d4r species is thermal stable up to 300 °C. ITQ-12 with F−@d4r shows substantial catalytic activity in Biginelli reaction. Furthermore, the catalytic performance is proved to be positive correlated with the presence of F−@d4r, indicating the mild acid catalytic property of F−@d4r.
The Effect of Different Pretreatment of Chicken Manure for Electricity Generation in Membrane-Less Microbial Fuel Cell
Authors: Mohd Azmi Nurhazirah , Mohd Sabri Muhammad Najib Ikmal , Tajarudin Husnul Azan , Shoparwe Noor Fazliani , Makhtar Muaz Mohd Zaini , Shukor Hafiza , Alam Mahboob , Siddiqui Masoom Raza , Rafatullah Mohd
Published: July 2022

The need for energy resources is growing all the time, which means that more fossil fuels are needed to provide them. People prefer to consume chicken as a source of protein, and this creates an abundance of waste. Thus, microbial fuel cells represent a new technological approach with the potential to generate electricity through the action of electrogenic bacteria toward chicken manure, while reducing the abundance of chicken manure. This study investigated the effect of different pretreatment (thermal, alkaline, and sonication pretreatment) of chicken manure to improve the performance of a membrane-less microbial fuel cell (ML-MFC). Statistical response surface methodology (RSM) through a central composite design (CCD) under a quadratic model was conducted for optimization of the ML-MFC performance focusing on the COD removal efficiency (R2 = 0.8917), biomass (R2 = 0.9101), and power density response (R2 = 0.8794). The study demonstrated that the highest COD removal (80.68%), biomass (7.8539 mg/L), and power density (220 mW/m2) were obtained when the pretreatment conditions were 140 °C, 20 kHz, and pH 10. The polarization curve of the best condition of ML-MFC was plotted to classify the behavior of the ML-MFC. The kinetic growth of Bacillus subtillis (BS) showed that, in treated chicken manure, the specific growth rate µ = 0.20 h−1 and doubling time Td = 3.43 h, whereas, in untreated chicken manure, µ = 0.11 h−1 and Td = 6.08.
Investigation of Phosphorus Loaded V2O5/ZrO2 Catalysts for the Oxidative Dehydrogenation of Propane (ODH)
Authors: Benzaouak Abdellah , Mahir Hanane , El Hamidi Adnane , Kacimi Mohamed , Liotta Leonarda Francesca
Published: July 2022

In this study, V2O5/ZrO2 samples loaded with different wt% of V2O5, ranging between 0% and 20% (wt% = 2.5, 3.6, 7.5, 10, and 20), were prepared and studied in the dehydrogenation of 2-butanol in order to investigate their acid-basic properties and to select the most interesting sample, that was identified in the 3.6 wt%V2O5/ZrO2. Such a catalyst was modified by adding phosphate at different atomic ratios (P/V = 0.5, 1, and 2) and further characterized by XRD, SEM-EDX, ESR, UV-Vis-PIR diffuse reflectance. Tests of catalytic dehydrogenation of 2-butanol were also performed. Then, the so-prepared samples were investigated in the oxidative dehydrogenation (ODH) of propane that represents the reaction of main interest in this study. It has been shown that the introduction of 3.6 wt%V2O5 and phosphate in the zirconia matrix enhances the stability of the tetragonal structure, improves acidity, and promotes ODH activity. Compared to the unpromoted 3.6 wt%V2O5/ZrO2 catalyst, the addition of phosphate increases the overall propane conversion from 12% to 20%, and also the propylene selectivity from 54% to near 64%, in the experimental conditions F °C3H8/F°O2/F°total (cm3/min): 3.6/1.8/60 at the temperature of 500 °C. The influence of the reaction mixture on the ODH, in particular the oxygen flow rate, was addressed. Highlights: Phosphorus loaded V2O5/ZrO2 catalysts were prepared and investigated in the oxidative dehydrogenation of propane. Addition of V2O5 and phosphorus to ZrO2 stabilized the tetragonal phase with respect to the monoclinic one. Among the prepared V2O5/ZrO2 samples, the most active catalyst corresponds to 3.6 wt% of V2O5/ ZrO2, The addition of phosphorus to 3.6 wt% V2O5/ZrO2 improves acidity and selectivity to propylene. Correlation between catalysts acidity and oxidative dehydrogenation of propane was observed.
The Effect of Chlorine Modification of Precipitated Iron Catalysts on Their Fischer–Tropsch Synthesis Properties
Authors: Li Weizhen , Zhang Xuebing , Wang Tao , Zhang Xiaoyu , Wei Linlin , Lin Quan , Lv Yijun , Men Zhuowu
Published: July 2022

Precipitated iron Fischer–Tropsch synthesis catalysts impregnated with chlorine were prepared and their Fischer–Tropsch synthesis performances were tested in a 1 L stirred tank reactor. The results showed that the chlorine modification had a significant influence on the Fischer–Tropsch synthesis performance of the precipitated iron catalyst. Compared with the catalyst without the chlorine modification, the catalyst containing about 0.1 wt% chlorine was deactivated by about 40% and the catalyst containing about 1 wt% chlorine was deactivated by about 65%. The textural properties, phase, reduction properties, and chlorine adsorption state of the catalysts before and after the Fischer–Tropsch synthesis were characterized. The strong interaction between chlorine and iron in the catalyst hindered the reduction and carbonization of the catalyst, which was the reason for the deactivation of the catalyst caused by the chlorine modification.
Multifunctional Role of S100 Protein Family in the Immune System: An Update
Authors: Singh Parul , Ali Syed Azmal
Published: July 2022

S100 is a broad subfamily of low-molecular weight calcium-binding proteins (9–14 kDa) with structural similarity and functional discrepancy. It is required for inflammation and cellular homeostasis, and can work extracellularly, intracellularly, or both. S100 members participate in a variety of activities in a healthy cell, including calcium storage and transport (calcium homeostasis). S100 isoforms that have previously been shown to play important roles in the immune system as alarmins (DAMPs), antimicrobial peptides, pro-inflammation stimulators, chemo-attractants, and metal scavengers during an innate immune response. Currently, during the pandemic, it was found that several members of the S100 family are implicated in the pathophysiology of COVID-19. Further, S100 family protein members were proposed to be used as a prognostic marker for COVID-19 infection identification using a nasal swab. In the present review, we compiled the vast majority of recent studies that focused on the multifunctionality of S100 proteins in the complex immune system and its associated activities. Furthermore, we shed light on the numerous molecular approaches and signaling cascades regulated by S100 proteins during immune response. In addition, we discussed the involvement of S100 protein members in abnormal defense systems during the pathogenesis of COVID-19.