bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Dynamic Parameter Adaptation in the Bird Swarm Algorithm Using General Type-2 Fuzzy Analysis and Mathematical Functions
Authors: Miramontes Ivette , Melin Patricia
Published: August 2023

The pursuit of continuous improvement across diverse processes presents a pressing challenge. Precision in manufacturing, efficient delivery route planning, and accurate diagnostics are imperative, prompting the exploration of innovative solutions. Nature-inspired algorithms offer a pathway for enhancing these processes. In this study, we address this challenge by dynamically adapting parameters in the Bird Swarm Algorithm using General Type-2 Fuzzy Systems, encompassing a range of rules and membership functions. Two complex case studies validate the effectiveness of our approach. The first evaluates Congress of Evolutionary Competition 2017 functions, while the second tackles the intricacies of Congress of Evolutionary Competition 2019 functions. Our methodology achieves an 97% improvement for Congress of Evolutionary Competition 2017 functions and a significant 70% enhancement for Congress of Evolutionary Competition 2019 functions. Notably, our results are benchmarked against the original method. Crucially, rigorous statistical analysis underscores the significant advancements facilitated by our proposed method. The comparison demonstrates clear and statistically significant improvements over the original approach. This study proves the marked impact of integrating General Type-2 Fuzzy Systems into the Bird Swarm Algorithm, presenting a promising avenue for addressing intricate optimization challenges in diverse domains.
Nonlinear Differential Equations of Flow Motion Considering Resistance Forces
Authors: Evtushenko Sergej , Kokhanenko Victor , Burtseva Olga
Published: August 2023

For a stationary potential 2D planar open high-velocity water flow of the ideal liquid, we propose a closed system of nonlinear equations considering the resistance forces to the flow from the channel bottom. Tangential stresses on jet interfaces are ignored. The resistance force components are expressed in terms of velocity components. In this case, the flow equations can be solved through the method of characteristics, and the surface forces are reduced to equivalent volumetric forces. The system of non-linear equations is solved in the velocity hodograph plane; further, the transition to the physical plane takes place. Since the value of the hydrodynamic pressure decreases downstream of the flow, the friction forces to the flow in the first approximation can be considered by using the integral laws of resistance. At that, the form of the equations of motion in the plane of the velocity hodograph does not change. This fact is proved in the article. An example of calculating the water flow is provided. The kinecity, ordinates, and velocities of the flow along its extreme line are calculated without considering resistance forces. Validation of the model in the real flow is performed. Acceptable accuracy relative to experimental data is obtained.
On the Geometry of the Riemannian Curvature Tensor of Nearly Trans-Sasakian Manifolds
Authors: Rustanov Aligadzhi R.
Published: August 2023

This paper presents the results of fundamental research into the geometry of the Riemannian curvature tensor of nearly trans-Sasakian manifolds. The components of the Riemannian curvature tensor on the space of the associated G-structure are counted, and the components of the Ricci tensor are calculated. Some identities are obtained that are satisfied by the Riemannian curvature tensors and the Ricci tensor. A number of properties are proved that characterize nearly trans-Sasakian manifolds with a closed contact form. The structure of nearly trans-Sasakian manifolds with a closed contact form is obtained. Several classes are singled out in terms of second-order differential-geometric invariants, and their local structure is obtained. The k-nullity distribution of a nearly trans-Sasakian manifold is studied.
Rational Design of Bismuth Metal Anodes for Sodium-/Potassium-Ion Batteries: Recent Advances and Perspectives
Authors: Wang Yan , Xu Xijun , Li Fangkun , Ji Shaomin , Zhao Jingwei , Liu Jun , Huo Yanping
Published: August 2023

Sodium-ion batteries (SIBs) and potassium-ion batteries (PIBs) have drawn widespread attention for application in large-scale accumulation energy because of their plentiful resources and lower cost. However, the lack of anodes with high energy density and long cycle lifetimes has hampered the progress of SIBs and PIBs. Bismuth (Bi), an alloying-type anode, on account of its high volumetric capacity and cost advantage, has become the most potential candidate for SIBs and PIBs. Nevertheless, Bi anodes undergo significant volume strain during the insertion and extraction of ions, resulting in the crushing of structures and a volatile solid electrolyte interface (SEI). As a result, the tactics to boost the electrochemical properties of Bi metal anodes in recent years are summarized in this study. Recent advances in designing nanostructure Bi-based materials are reviewed, and the reasonable effects of architectural design and compound strategy on the combination property are discussed. Some reasonable strategies and potential challenges for the design of Bi-based materials are also summarized. This review aims to provide practical guidance for the development of alloying-type anode materials for next-generation SIBs and KIBs.
Remaining Useful Life Prediction of Lithium-Ion Batteries Based on a Cubic Polynomial Degradation Model and Envelope Extraction
Authors: Su Kangze , Deng Biao , Tang Shengjin , Sun Xiaoyan , Fang Pengya , Si Xiaosheng , Han Xuebing
Published: August 2023

Remaining useful life (RUL) prediction has become one of the key technologies for reducing costs and improving safety of lithium-ion batteries. To our knowledge, it is difficult for existing nonlinear degradation models of the Wiener process to describe the complex degradation process of lithium-ion batteries, and there is a problem with low precision in parameter estimation. Therefore, this paper proposes a method for predicting the RUL of lithium-ion batteries based on a cubic polynomial degradation model and envelope extraction. Firstly, based on the degradation characteristics of lithium-ion batteries, a cubic polynomial function is used to fit the degradation trajectory and compared with other nonlinear degradation models for verification. Secondly, a subjective parameter estimation method based on envelope extraction is proposed that estimates the actual degradation trajectory by using the average of the upper and lower envelope curves of the degradation data of lithium-ion batteries and uses the maximum likelihood estimation (MLE) method to estimate the unknown model parameters in two steps. Finally, for comparison with several typical nonlinear models, experiments are carried out based on the practical degradation data of lithium-ion batteries. The effectiveness of the proposed method to improve the accuracy of RUL prediction for lithium-ion batteries was demonstrated in terms of the mean square error (MSE) of the model and MSE of RUL prediction.
Effect of Maturation with American Oak Chips on the Volatile and Sensory Profile of a Cabernet Sauvignon Rosé Wine and Its Comparison with Commercial Wines
Authors: Hernández-Carapia Miguel Ángel , Verde-Calvo José Ramón , Escalona-Buendía Héctor Bernardo , Peña-Álvarez Araceli
Published: August 2023

Rosé wines are commonly consumed as young wines mainly due to their freshness and fruity character. Nevertheless, in recent years a new market looking for alternatives to traditional wines has emerged. Considering this, the study of the volatile and sensory profiles of a varietal rosé wine aged with oak chips was carried out. Two Cabernet Sauvignon rosé wines were made: one was maturated with oak chips and the other without. Both wines were physicochemically characterized. Then, their volatile and sensory profiles were analyzed, also including two commercial wines. The results showed that the produced wines complied with Mexican regulations. Also, they showed greater relative areas in compounds such as ethyl (E)-2-hexenoate, ethyl heptanoate, ethyl nonanoate, ethyl 3-nonenoate, β-citronellol, (±)-trans-nerolidol, and β-damascenone. In their sensory profile, they were mostly related to attributes such as berries, prune, bell pepper, and herbaceous notes. Among the compounds related to barrel maturation, only cis-oak-lactone was identified in the rosé wine matured with chips. However, it was associated with vanilla, woody, smoky, and spicy attributes. According to the results, the maturation of rosé wines with oak chips could be a good alternative to provide them with unusual notes and thus offer new alternatives to traditional and new wine consumers.
Physiologically Based Pharmacokinetic Modeling of Extracellular Vesicles
Authors: Kumar Prashant , Mehta Darshan , Bissler John J.
Published: August 2023

Extracellular vesicles (EVs) are lipid membrane bound-cell-derived structures that are a key player in intercellular communication and facilitate numerous cellular functions such as tumor growth, metastasis, immunosuppression, and angiogenesis. They can be used as a drug delivery platform because they can protect drugs from degradation and target specific cells or tissues. With the advancement in the technologies and methods in EV research, EV-therapeutics are one of the fast-growing domains in the human health sector. Therapeutic translation of EVs in clinics requires assessing the quality, safety, and efficacy of the EVs, in which pharmacokinetics is very crucial. We report here the application of physiologically based pharmacokinetic (PBPK) modeling as a principal tool for the prediction of absorption, distribution, metabolism, and excretion of EVs. To create a PBPK model of EVs, researchers would need to gather data on the size, shape, and composition of the EVs, as well as the physiological processes that affect their behavior in the body. The PBPK model would then be used to predict the pharmacokinetics of drugs delivered via EVs, such as the rate at which the drug is absorbed and distributed throughout the body, the rate at which it is metabolized and eliminated, and the maximum concentration of the drug in the body. This information can be used to optimize the design of EV-based drug delivery systems, including the size and composition of the EVs, the route of administration, and the dose of the drug. There has not been any dedicated review article that describes the PBPK modeling of EV. This review provides an overview of the absorption, distribution, metabolism, and excretion (ADME) phenomena of EVs. In addition, we will briefly describe the different computer-based modeling approaches that may help in the future of EV-based therapeutic research.
Tree Physiological Variables as a Proxy of Heavy Metal and Platinum Group Elements Pollution in Urban Areas
Authors: Varela Zulema , Martínez-Abaigar Javier , Tomás-Las-Heras Rafael , Fernández José Ángel , Del-Castillo-Alonso María-Ángeles , Núñez-Olivera Encarnación
Published: August 2023

Physiological variables (the content of chlorophyll, flavonoids and nitrogen, together with Fv/Fm) and the content of ten heavy metals (As, Cd, Cu, Hg, Mn, Ni, Pb, Sb, V and Zn) and two platinum group elements (PGEs: Pd and Rh) were measured in the leaves of 50 individuals of Ligustrum lucidum trees regularly distributed in the city of Logroño (Northern Spain). Three of these variables increased with increasing physiological vitality (chlorophyll, nitrogen and Fv/Fm), whereas flavonoids increased in response to different abiotic stresses, including pollution. Our aim was to test their adequacy as proxies for the pollution due to heavy metals and PGEs. The three vitality indicators generally showed high values typical of healthy plants, and they did not seem to be consistently affected by the different pollutants. In fact, the three vitality variables were positively correlated with the first factor of a PCA that was dominated by heavy metals (mainly Pb, but also Sb, V and Ni). In addition, Fv/Fm was negatively correlated with the second factor of the PCA, which was dominated by PGEs, but the trees showing Fv/Fm values below the damage threshold did not coincide with those showing high PGE content. Regarding flavonoid content, it was negatively correlated with PCA factors dominated by heavy metals, which did not confirm its role as a protectant against metal stress. The relatively low levels of pollution usually found in the city of Logroño, together with the influence of other environmental factors and the relative tolerance of Ligustrum lucidum to modest atmospheric pollution, probably determined the only slight response of the physiological variables to heavy metals and PGEs.
Measurement of Salivary Cortisol in Two New World Primate Species
Authors: Stocker Martina , O’Sullivan Eoin P. , Palme Rupert , Millesi Eva , Sonnweber Ruth
Published: August 2023

Glucocorticoids (GCs) are mammalian steroid hormones involved in a variety of physiological processes, including metabolism, the immune response, and cardiovascular functions. Due to their link to the physiological stress response, GC measurement is a valuable tool for conservation and welfare assessment in animal populations. GC levels can be measured from different matrices, such as urine and feces. Moreover, especially in captive settings, measuring GCs from saliva samples proved particularly useful as those samples can be collected non-invasively and easily from trained animals. Salivary GC levels can be measured using a variety of analytical methods, such as enzyme immunoassays. However, it is crucial to validate the analytical method for each specific application and species when using a new matrix. Using high-pressure liquid chromatography and a cortisol enzyme immunoassay, we show that the main glucocorticoids secreted in the saliva of squirrel monkeys and brown capuchin monkeys are cortisol and cortisone. Our biological validation found the expected salivary cortisol level to decline throughout the day. Our findings support the reliability of salivary cortisol measurements and their potential to be used as a valid tool in research and welfare assessment for these non-human primates.
Transcriptome Responses to Different Environments in Intertidal Zones in the Peanut Worm Sipunculus nudus
Authors: Li Junwei , Wen Jiufu , Hu Ruiping , Pei Surui , Li Ting , Shan Binbin , Huang Honghui , Zhu Changbo
Published: August 2023

The peanut worm (Sipunculus nudus) is an important intertidal species worldwide. Species living in the same aquaculture area might suffer different environmental impacts. To increase knowledge of the molecular mechanisms underlying the response to environmental fluctuations, we performed a transcriptome analysis of S. nudus from different intertidal zones using a combination of the SMRT platform and the Illumina sequencing platform. (1) A total of 105,259 unigenes were assembled, and 23,063 unigenes were perfectly annotated. The results of the PacBio Iso-Seq and IIIumina RNA-Seq enriched the genetic database of S. nudus. (2) A total of 830 DEGs were detected in S. nudus from the different groups. In particular, 33 DEGs had differential expression in the top nine KEGG pathways related to pathogens, protein synthesis, and cellular immune response and signaling. The results indicate that S. nudus from different zones experience different environmental stresses. (3) Several DEGs (HSPA1, NFKBIA, eEF1A, etc.) in pathways related to pathogens (influenza A, legionellosis, measles, and toxoplasmosis) had higher expression in groups M and L. HSPA1 was clearly enriched in most of the pathways, followed by NFKBIA. The results show that the peanut worms from the M and L tidal flats might have suffered more severe environmental conditions. (4) Some DEGs (MKP, MRAS, and HSPB1) were upregulated in peanut worms from the H tidal flat, and these DEGs were mainly involved in the MAPK signaling pathway. These results indicate that the MAPK pathway may play a vital role in the immune response of the peanut worm to the effects of different intertidal flats. This study provides a valuable starting point for further studies to elucidate the molecular basis of the response to different environmental stresses in S. nudus.
Hyperglycemia and Hyperlipidemia with Kidney or Liver Transplantation: A Review
Authors: D’Elia John A. , Weinrauch Larry A.
Published: August 2023

Although solid organ transplantation in persons with diabetes mellitus is often associated with hyperglycemia, the risk of hyperlipidemia in all organ transplant recipients is often underestimated. The diagnosis of diabetes often predates transplantation; however, in a moderate percentage of allograft recipients, perioperative hyperglycemia occurs triggered by antirejection regimens. Post-transplant prescription of glucocorticoids, calcineurin inhibitors and mTOR inhibitors are associated with increased lipid concentrations. The existence of diabetes mellitus prior to or following a liver transplant is associated with shorter times of useful allograft function. A cycle involving Smad, TGF beta, m-TOR and toll-like receptors has been identified in the contribution of rejection and aging of allografts. Glucocorticoids (prednisone) and calcineurin inhibitors (cyclosporine and tacrolimus) induce hyperglycemia associated with insulin resistance. Azathioprine, mycophenolate and prednisone are associated with lipogenesis. mTOR inhibitors (rapamycin) are used to decrease doses of atherogenic agents used for immunosuppression. Post-transplant medication management must balance immune suppression and glucose and lipid control. Concerns regarding rejection often override those relative to systemic and organ vascular aging and survival. This review focuses attention on the underlying mechanism of relationships between glycemia/lipidemia control, transplant rejection and graft aging.
In Silico Analysis of Pyeongwi-San Involved in Inflammatory Bowel Disease Treatment Using Network Pharmacology, Molecular Docking, and Molecular Dynamics
Authors: Bae Chang-Hwan , Kim Hee-Young , Seo Ji Eun , Lee Hanul , Kim Seungtae
Published: August 2023

Backgound: Pyeongwi-san (PWS) is a widely used formula for treating digestive disorders in Korea and China. Inflammatory bowel disease (IBD) is characterized by progressive inflammation of the gastrointestinal tract. Emerging evidence supports the protective effect of PWS against IBD, but specific mechanisms are still elusive. Methods: Active compounds of PWS were screened from the medicinal materials and chemical compounds in Northeast Asian traditional medicine (TM-MC) in the consideration of drug-likeness and oral bioavailability. Target candidates of active compounds were predicted using the ChEMBL database. IBD-related targets were obtained from the GeneCards and DisGeNET databases. The network of composition-targets-disease was constructed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were analyzed. Molecular docking was used to simulate the binding affinity of active compounds on target proteins and molecular dynamics was used to validate the molecular docking result. Results: A total of 26 core target proteins of PWS were related to IBD. Enrichment analysis suggested that PWS is highly associated with tumor necrosis factor signaling pathway, apoptosis, and the collapse of tight junctions. Moreover, molecular docking and molecular dynamics simulation proposed β-eudesmol and (3R,6R,7S)-1,10-bisaboladien-3-ol to ameliorate IBD through the binding to TNF and MMP9, respectively. Conclusion: Present in silico analysis revealed potential pathways and insight of PWS to regulate IBD. These results imply that the therapeutic effect of PWS might be achieved via an inhibitory effect.
Low Valine Serum Levels Predict Increased 1-Year Mortality in Acute Heart Failure Patients
Authors: Klobučar Iva , Vidović Luka , Arih Ilona , Lechleitner Margarete , Pregartner Gudrun , Berghold Andrea , Habisch Hansjörg , Madl Tobias , Frank Saša , Degoricija Vesna
Published: August 2023

Considering the relationship between disease severity and the extent of metabolic derangement in heart failure, we hypothesized that the serum levels of metabolites may have prognostic value for 1-year mortality in acute heart failure (AHF). The AHF study was a prospective, observational study enrolling consecutive patients hospitalized due to AHF. Metabolites were measured in serum collected at admission using NMR spectroscopy. Out of 315 AHF patients, 118 (37.5%) died within 1 year after hospitalization for AHF. The serum levels of 8 out of 49 identified metabolites were significantly different between patients who were alive and those who died within 1 year after hospitalization for AHF. Of these, only valine was significantly associated with 1-year mortality (hazard ratio 0.73 per 1 standard deviation increase, 95% confidence interval: 0.59–0.90, p = 0.003) in the multivariable Cox regression analyses. Kaplan–Maier analysis showed significantly higher survival rates in AHF patients with valine levels above the median (>279.2 µmol/L) compared to those with valine levels ≤ 279.2 µmol/L. In a receiver operating characteristics curve analysis, valine was able to discriminate between the two groups with an area under the curve of 0.65 (95% CI 0.59–0.72). We conclude that valine serum levels might be of prognostic value in AHF.
Low-Dose Chidamide Treatment Displays Sex-Specific Differences in the 3xTg-AD Mouse
Authors: Dennison Jessica , Mendez Armando , Szeto Angela , Lohse Ines , Wahlestedt Claes , Volmar Claude-Henry
Published: August 2023

Epigenetic compounds have become attractive small molecules for targeting the multifaceted aspects of Alzheimer’s disease (AD). Although AD disproportionately affects women, most of the current literature investigating epigenetic compounds for the treatment of AD do not report sex-specific results. This is remarkable because there is rising evidence that epigenetic compounds intrinsically affect males and females differently. This manuscript explores the sexual dimorphism observed after chronic, low-dose administration of a clinically relevant histone deacetylase inhibitor, chidamide (Tucidinostat), in the 3xTg-AD mouse model. We found that chidamide treatment significantly improves glucose tolerance and increases expression of glucose transporters in the brain of males. We also report a decrease in total tau in chidamide-treated mice. Differentially expressed genes in chidamide-treated mice were much greater in males than females. Genes involved in the neuroinflammatory pathway and amyloid processing pathway were mostly upregulated in chidamide-treated males while downregulated in chidamide-treated females. This work highlights the need for drug discovery projects to consider sex as a biological variable to facilitate translation.
Neuroactive Steroid–Gut Microbiota Interaction in T2DM Diabetic Encephalopathy
Authors: Diviccaro Silvia , Cioffi Lucia , Piazza Rocco , Caruso Donatella , Melcangi Roberto Cosimo , Giatti Silvia
Published: August 2023

The pathological consequences of type 2 diabetes mellitus (T2DM) also involve the central nervous system; indeed, T2DM patients suffer from learning and memory disabilities with a higher risk of developing dementia. Although several factors have been proposed as possible contributors, how neuroactive steroids and the gut microbiome impact brain pathophysiology in T2DM remain unexplored. On this basis, in male Zucker diabetic fatty (ZDF) rats, we studied whether T2DM alters memory abilities using the novel object recognition test, neuroactive steroid levels by liquid chromatography–tandem mass spectrometry, hippocampal parameters using molecular assessments, and gut microbiome composition using 16S next-generation sequencing. Results obtained reveal that T2DM worsens memory abilities and that these are correlated with increased levels of corticosterone in plasma and with a decrease in allopregnanolone in the hippocampus, where neuroinflammation, oxidative stress, and mitochondrial dysfunction were reported. Interestingly, our analysis highlighted a small group of taxa strictly related to both memory impairment and neuroactive steroid levels. Overall, the data underline an interesting role for allopregnanolone and microbiota that may represent candidates for the development of therapeutic strategies.
Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia
Authors: De Piano Maria , Cacciamani Andrea , Balzamino Bijorn Omar , Scarinci Fabio , Cosimi Pamela , Cafiero Concetta , Ripandelli Guido , Micera Alessandra
Published: August 2023

Inflammatory, vasculogenic, and profibrogenic factors have been previously reported in vitreous (VH) and aqueous (AH) humors in myopic patients who underwent cataract surgery. In light of this, we selected some mediators for AH and anterior-capsule-bearing lens epithelial cell (AC/LEC) analysis, and AH expression was correlated with LEC activation (epithelial–mesenchymal transition and EMT differentiation) and axial length (AL) elongation. In this study, AH (97; 41M/56F) and AC/LEC samples (78; 35M/43F) were collected from 102 patients who underwent surgery, and biosamples were grouped according to AL elongation. Biomolecular analyses were carried out for AH and LECs, while microscopical analyses were restricted to whole flattened AC/LECs. The results showed increased levels of interleukin (IL)-6, IL-8, and angiopoietin-2 (ANG)-2 and decreased levels of vascular endothelium growth factor (VEGF)-A were detected in AH depending on AL elongation. LECs showed EMT differentiation as confirmed by the expression of smooth muscle actin (α-SMA) and transforming growth factor (TGF)-βR1/TGFβ isoforms. A differential expression of IL-6R/IL-6, IL-8R/IL-8, and VEGF-R1/VEGF was observed in the LECs, and this expression correlated with AL elongation. The higher VEGF-A and lower VEGF-D transcript expressions were detected in highly myopic LECs, while no significant changes were monitored for VEGF-R transcripts. In conclusion, these findings provide a strong link between the AH protein signature and the EMT phenotype. Furthermore, the low VEGF-A/ANG-2 and the high VEGF-A/VEGF-D ratios in myopic AH might suggest a specific inflammatory and profibrogenic pattern in high myopia. The highly myopic AH profile might be a potential candidate for rating anterior chamber inflammation and predicting retinal distress at the time of cataract surgery.
Involvement of the AKT Pathway in Resistance to Erlotinib and Cabozantinib in Triple-Negative Breast Cancer Cell Lines
Authors: Lefebvre Cory , Pellizzari Sierra , Bhat Vasudeva , Jurcic Kristina , Litchfield David W. , Allan Alison L.
Published: August 2023

Resistance to protein tyrosine kinase inhibitors (TKIs) presents a significant challenge in therapeutic target development for cancers such as triple-negative breast cancer (TNBC), where conventional therapies are ineffective at combatting systemic disease. Due to increased expression, the receptor tyrosine kinases EGFR (epidermal growth factor receptor) and c-Met are potential targets for treatment. However, targeted anti-EGFR and anti-c-Met therapies have faced mixed results in clinical trials due to acquired resistance. We hypothesize that adaptive responses in regulatory kinase networks within the EGFR and c-Met signaling axes contribute to the development of acquired erlotinib and cabozantinib resistance. To test this, we developed two separate models for cabozantinib and erlotinib resistance using the MDA-MB-231 and MDA-MB-468 cell lines, respectively. We observed that erlotinib- or cabozantinib-resistant cell lines demonstrate enhanced cell proliferation, migration, invasion, and activation of EGFR or c-Met downstream signaling (respectively). Using a SILAC (Stable Isotope Labeling of Amino acids in Cell Culture)-labeled quantitative mass spectrometry proteomics approach, we assessed the effects of erlotinib or cabozantinib resistance on the phosphoproteome, proteome, and kinome. Using this integrated proteomics approach, we identified several potential kinase mediators of cabozantinib resistance and confirmed the contribution of AKT1 to erlotinib resistance in TNBC-resistant cell lines.
Protein-Bound Uremic Toxins in Senescence and Kidney Fibrosis
Authors: Yang Yi , Mihajlovic Milos , Masereeuw Rosalinde
Published: August 2023

Chronic kidney disease (CKD) is a progressive condition of kidney dysfunction due to diverse causes of injury. In healthy kidneys, protein-bound uremic toxins (PBUTs) are cleared from the systemic circulation by proximal tubule cells through the concerted action of plasma membrane transporters that facilitate their urinary excretion, but the endogenous metabolites are hardly removed with kidney dysfunction and may contribute to CKD progression. Accumulating evidence suggests that senescence of kidney tubule cells influences kidney fibrosis, the common endpoint for CKD with an excessive accumulation of extracellular matrix (ECM). Senescence is a special state of cells characterized by permanent cell cycle arrest and limitation of proliferation, which promotes fibrosis by releasing senescence-associated secretory phenotype (SASP) factors. The accumulation of PBUTs in CKD causes oxidative stress and increases the production of inflammatory (SASP) factors that could trigger fibrosis. Recent studies gave some clues that PBUTs may also promote senescence in kidney tubular cells. This review provides an overview on how senescence contributes to CKD, the involvement of PBUTs in this process, and how kidney senescence can be studied. Finally, some suggestions for future therapeutic options for CKD while targeting senescence are given.
Washed Microbiota Transplantation Improves Patients with Overweight by the Gut Microbiota and Sphingolipid Metabolism
Authors: Wu Lei , Lin Zi-Han , Lu Xin-Jian , Hu Xuan , Zhong Hao-Jie , Lin De-Jiang , Liu Tao , Xu Jia-Ting , Lin Wen-Ying , Wu Qing-Ping , He Xing-Xiang
Published: August 2023

Background: Overweight (OW) and obesity have become increasingly serious public health problems worldwide. The clinical impact of washed microbiota transplantation (WMT) from healthy donors in OW patients is unclear. This study aimed to investigate the effect of WMT in OW patients. Methods: The changes in body mass index (BMI = weight (kg)/height (m)2), blood glucose, blood lipids and other indicators before and after WMT were compared. At the same time, 16S rRNA gene amplicon sequencing was performed on fecal samples of OW patients before and after transplantation. Finally, serum samples were tested for sphingolipids targeted by lipid metabolomics. Results: A total of 166 patients were included, including 52 in the OW group and 114 in the normal weight (NOW) group. For OW patients, WMT significantly improved the comprehensive efficacy of OW. In the short term (about 1 month) and medium term (about 2 months), a significant reduction in BMI was seen. At the same time, in the short term (about 1 month), liver fat attenuation (LFA), triglyceride (TG) and fasting blood glucose (FBG) were significantly reduced. In the long term (about 5 months), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), non-high-density lipoprotein (non-HDL-c), etc. were significantly reduced. WMT improved the gut microbiota of OW patients, and also had an improvement effect on OW patients by regulating sphingolipid metabolism. Conclusion: WMT had a significant improvement effect on OW patients. WMT could restore gut microbiota homeostasis and improve OW patients by regulating sphingolipid metabolism.
Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors
Authors: Lee Minhyuk , Kang Seungjae , Kim Sungjee , Park Nokyoung
Published: August 2023

miRNAs are endogenous small, non-coding RNA molecules that function in post-transcriptional regulation of gene expression. Because miRNA plays a pivotal role in maintaining the intracellular environment, and abnormal expression has been found in many cancer diseases, detection of miRNA as a biomarker is important for early diagnosis of disease and study of miRNA function. However, because miRNA is present in extremely low concentrations in cells and many types of miRNAs with similar sequences are mixed, traditional gene detection methods are not suitable for miRNA detection. Therefore, in order to overcome this limitation, a signal amplification process is essential for high sensitivity. In particular, enzyme-free signal amplification systems such as DNAzyme systems have been developed for miRNA analysis with high specificity. DNAzymes have the advantage of being more stable in the physiological environment than enzymes, easy to chemically synthesize, and biocompatible. In this review, we summarize and introduce the methods using DNAzyme-based biosensors, especially with regard to various signal amplification methods for high sensitivity and strategies for improving detection specificity. We also discuss the current challenges and trends of these DNAzyme-based biosensors.
The Impact of Limited Previous Motor Experience on Action Possibility Judgments in People with Spinal Muscle Atrophy
Authors: Heirani Moghaddam Sarvenaz , Sen Dilara , Carson Megan , Mackowiak Robert , Markley Rachel , Manson Gerome Aleandro
Published: August 2023

Previous studies have shown that people with limited motor capabilities may rely on previous motor experience when making action possibility judgments for others. In the present study, we examined if having limited previous motor experience, as a consequence of spinal muscle atrophy (SMA), alters action possibility judgments. Participants with SMA and neurologically healthy (NH) sex- and age-matched controls performed a perceptual-motor judgment task using the Fitts’s law paradigm. Participants observed apparent motion videos of reciprocal aiming movements with varying levels of difficulty. For each movement, participants predicted the shortest movement time (MT) at which a neurologically healthy young adult could accurately perform the task. Participants with SMA predicted significantly longer MTs compared to controls; however, the predicted MTs of both SMA and NH participants exhibited a Fitts’s law relationship (i.e., the predicted MTs significantly increased as movement difficulty increased). Overall, these results provide evidence that participants with SMA who have limited, or no motor experience may make more conservative action possibility judgments for others. Critically, our finding that the pattern of action possibility judgments was not different between SMA and NH groups suggests that limited previous motor experience may not completely impair action possibility judgments.
What Is the Contribution of Community Programs to the Physical Activity of Women? A Study Based on Public Open Spaces in Brazil
Authors: Funez Eduardo Irineu Bortoli , da Silva Alice Tatiane , dos Santos Letícia Pechnicki , Rodriguez-Añez Ciro Romelio , de Paula da Silva Alexandre Augusto , Fermino Rogério César
Published: August 2023

Community programs can facilitate the access of vulnerable subgroups to physical activity (PA). This study analyzed the relationship between sociodemographic characteristics, health conditions, public open spaces (POS) usage, and women’s PA. The 155 participants were assiduous in taking part in PA classes in POS in São José dos Pinhais, Brazil. The accelerometer-based PA measures identified four outcomes: (1) daily light-intensity PA (LPA), (2) daily moderate-to-vigorous intensity PA (MVPA), (3) LPA in POS, and (4) MVPA in POS. Linear regression, and the Durbin–Watson and Mann–Whitney U tests were used for data analysis in STATA software. The main results showed that the weekly frequency (β: 10.9, p < 0.01) and intensity of the main activity in the POS (β: 22.4, p < 0.05) were related to daily MVPA. Economic level and length of stay in the POS were positively related to the LPA performed (p < 0.05). Weekly frequency (β: 2.4, p < 0.01), length of stay (β: 11.0, p < 0.01), and intensity of PA practiced in the POS (β: 5.9, p < 0.05) showed a positive relationship with MVPA in the POS. In conclusion, there was a positive relationship between some analyzed variables and PA of different intensities, especially the consistent relationship between weekly frequency of POS usage and MVPA. Participation in structured PA classes in a community program can contribute to an increase of 47 min of daily MVPA.
Experimental and Numerical Investigations of the Seismic Performance of Reinforced Concrete Frames Strengthened with CFRP Sheets
Authors: Wang Yao , Chen Weihong , Li Dong , Xu Hongguang , Zhang Feng , Guo Xiao
Published: August 2023

To study the seismic strengthening of damaged reinforced concrete (RC) frames using CFRP sheets, this study designed and tested the scaled 2-bay and 2-storey RC frame specimens. After applying a low cyclic horizontal load to simulate the initial damage to the specimen in an earthquake disaster, CFRP was used to strengthen the joints of the damaged RC frame. Pseudo-static tests of strengthened specimens and counterpart specimens were then carried out. Seismic performance, including stiffness, load capacity, ductility and energy dissipation were further analyzed. The failure mode of strengthened RC frame structures showed excellent ductility. The results demonstrated that the strengthening method involving wrapping CFRP can significantly improve the maximum horizontal bearing capacity, initial stiffness and energy dissipation capacity of the non-ductile reinforced concrete frame structure. The average displacement ductility coefficient of strengthened specimen can be enhanced to 3.41 compared with that of counterpart specimen (3.00). The pushover analysis based on the OpenSees model determined that the prototype frame with CFRP strengthening can maintain structural integrity and safety, with its maximum interstorey displacement angle below the limit of seismic specification (i.e., 1/50 in a severe earthquake). This study can contribute to the development of practical and efficient methods for restoring and improving the performance of damaged RC frames in seismic-prone regions.
Development of Free-Form Assembly-Type Mold Production Technology Using 3D Printing Technology
Authors: Youn Jongyoung , Cho Minwoo , Chae Hyuntak , Jeong Kyeongtae , Kim Sungjin , Do Sunglok , Lee Donghoon
Published: August 2023

Free-form molds are used for one-time curve configuration, and because they are produced through manpower, they have issues with reduced precision and the occurrence of errors. In this study, 3D printing technologies were used to ensure precision, and polylactic acid and reusable eco-friendly materials to develop free-form assembly-type side-mold production technologies. In verifying the side mold, a free-form concrete panel was produced to check whether deformation occurs due to lateral pressure. Therefore, in this study, to verify this, a free-form concrete panel was produced and 3D-scanned to analyze the error at the side mold and the cause of the error to confirm the performance of the mold. The results showed that the error at each part was small, with a standard deviation of 1.627 mm, and there was little error at the panel joint part, around 1°. Such research is expected to be used in studies related to mold production technologies using 3D printers and on the production of free-form side molds.
The Association between the Gut Microbiome and Development and Progression of Cancer Treatment Adverse Effects
Authors: Maddern Amanda S. , Coller Janet K. , Bowen Joanne M. , Gibson Rachel J.
Published: August 2023

Adverse effects are a common consequence of cytotoxic cancer treatments. Over the last two decades there have been significant advances in exploring the relationship between the gut microbiome and these adverse effects. Changes in the gut microbiome were shown in multiple clinical studies to be associated with the development of acute gastrointestinal adverse effects, including diarrhoea and mucositis. However, more recent studies showed that changes in the gut microbiome may also be associated with the long-term development of psychoneurological changes, cancer cachexia, and fatigue. Therefore, the aim of this review was to examine the literature to identify potential contributions and associations of the gut microbiome with the wide range of adverse effects from cytotoxic cancer treatments.
Role of Alpha-Fetoprotein (AFP) in Diagnosing Childhood Cancers and Genetic-Related Chronic Diseases
Authors: Głowska-Ciemny Joanna , Szymanski Marcin , Kuszerska Agata , Rzepka Rafał , von Kaisenberg Constantin S. , Kocyłowski Rafał
Published: August 2023

Alpha-fetoprotein (AFP) is a protein commonly found during fetal development, but its role extends beyond birth. Throughout the first year of life, AFP levels can remain high, which can potentially mask various conditions from the neurological, metabolic, hematological, endocrine, and early childhood cancer groups. Although AFP reference values and clinical utility have been established in adults, evaluating AFP levels in children during the diagnostic process, treatment, and post-treatment surveillance is still associated with numerous diagnostic pitfalls. These challenges arise from the presence of physiologically elevated AFP levels, inconsistent data obtained from different laboratory tests, and the limited population of children with oncologic diseases that have been studied. To address these issues, it is essential to establish updated reference ranges for AFP in this specific age group. A population-based study involving a statistically representative group of patients could serve as a valuable solution for this purpose.
The Relation of Lifestyle with Inflammation at the Time of Diagnosis in Patients with Colorectal Cancer
Authors: Gielen Anke H. C. , Melenhorst Jarno , Breukink Stephanie O. , Weijenberg Matty P. , Bours Martijn J. L.
Published: August 2023

Colorectal cancer is one of the most common lifestyle-related types of cancer. The exact pathophysiologic mechanism in the relation between (visceral) adipose tissue, systemic inflammation and colorectal cancer remains unknown. This study aimed to assess the association of lifestyle with markers of systemic inflammation at the time of diagnosis in stage I-III colorectal cancer patients. Patients (n = 298) with stage I-III colorectal cancer from three Dutch hospitals were included at diagnosis. Several lifestyle-related variables (MUST nutritional status score, WCRF/AICR healthy lifestyle score, active smoking, alcohol consumption and BMI) and inflammatory markers (plasma levels of IL-6, IL-8, IL-10, TNFα and ‘high sensitive’ hsCRP) were measured at the time of diagnosis. Confounder-adjusted multivariable linear regression models were used to analyse how the lifestyle variables were associated with the inflammatory markers. Statistically significant associations were found between a better WCRF/AICR lifestyle score and lower levels of IL-6 and hsCRP. A medium and high risk of malnutrition according to the MUST score was associated with elevated levels of both IL-8 and hsCRP. An overall unhealthier lifestyle indicated by a lower WCRF/AICR lifestyle score and a higher risk of malnutrition according to the MUST score at the time of diagnosis was associated with elevated levels of inflammatory markers. These findings can contribute to formulating lifestyle advice to improve treatment outcomes and prognosis in patients having CRC in the future.
Effects of Hyperthermia and Hyperthermic Intraperitoneal Chemoperfusion on the Peritoneal and Tumor Immune Contexture
Authors: Chia Daryl K. A. , Demuytere Jesse , Ernst Sam , Salavati Hooman , Ceelen Wim
Published: August 2023

Hyperthermia combined with intraperitoneal (IP) drug delivery is increasingly used in the treatment of peritoneal metastases (PM). Hyperthermia enhances tumor perfusion and increases drug penetration after IP delivery. The peritoneum is increasingly recognized as an immune-privileged organ with its own distinct immune microenvironment. Here, we review the immune landscape of the healthy peritoneal cavity and immune contexture of peritoneal metastases. Next, we review the potential benefits and unwanted tumor-promoting effects of hyperthermia and the associated heat shock response on the tumor immune microenvironment. We highlight the potential modulating effect of hyperthermia on the biomechanical properties of tumor tissue and the consequences for immune cell infiltration. Data from translational and clinical studies are reviewed. We conclude that (mild) hyperthermia and HIPEC have the potential to enhance antitumor immunity, but detailed further studies are required to distinguish beneficial from tumor-promoting effects.
Environmental and Lifestyle Cancer Risk Factors: Shaping Extracellular Vesicle OncomiRs and Paving the Path to Cancer Development
Authors: Bollati Valentina , Monti Paola , Biganzoli Davide , Marano Giuseppe , Favero Chiara , Iodice Simona , Ferrari Luca , Dioni Laura , Bianchi Francesca , Pesatori Angela Cecilia , Biganzoli Elia Mario
Published: August 2023

Intercellular communication has been transformed by the discovery of extracellular vesicles (EVs) and their cargo, including microRNAs (miRNAs), which play crucial roles in intercellular signaling. These EVs were previously disregarded as cellular debris but are now recognized as vital mediators of biological information transfer between cells. Furthermore, they respond not only to internal stimuli but also to environmental and lifestyle factors. Identifying EV-borne oncomiRs, a subset of miRNAs implicated in cancer development, could revolutionize our understanding of how environmental and lifestyle exposures contribute to oncogenesis. To investigate this, we studied the plasma levels of EV-borne oncomiRs in a population of 673 women and 238 men with a body mass index > 25 kg/m2 (SPHERE population). The top fifty oncomiRs associated with the three most common cancers in women (breast, colorectal, and lung carcinomas) and men (lung, prostate, and colorectal carcinomas) were selected from the OncomiR database. Only oncomiRs expressed in more than 20% of the population were considered for statistical analysis. Using a Multivariate Adaptive Regression Splines (MARS) model, we explored the interactions between environmental/lifestyle exposures and EV oncomiRs to develop optimized predictor combinations for each EV oncomiR. This innovative approach allowed us to better understand miRNA regulation in response to multiple environmental and lifestyle influences. By uncovering non-linear relationships among variables, we gained valuable insights into the complexity of miRNA regulatory networks. Ultimately, this research paves the way for comprehensive exposome studies in the future.
Customized High-Value Agricultural Residue Conversion: Applications in Wastewater Treatment
Authors: Tan Xuefei , Wang Huiwen , Guo Xiaoyan , Ho Shih-Hsin
Published: August 2023

Wastewater treatment methods based on catalytic technology are receiving more and more attention in recent years. In order to achieve the high-value utilization of agricultural residues, the development of greener, more efficient, and economically favorable catalysts is the focus of this review. The main contents include: (i) discussions around the differences in surface morphology, chemical stability, electrical conductivity, porosity, and the specific surface area of the prepared biochar according to the biochemical composition of different agricultural residues; (ii) through the above comparison, the shortlisting and selection of suitable catalysts for the preparation of agricultural residues to improve the adsorption and catalytic performance; (iii) discussions around the interaction mode and catalytic mechanism between the surface properties of biochar and pollutants; (iv) based upon future needs, discussions around making full use of the distribution of the pyrolysis products of agricultural residue, and designing biochar-based catalysts to achieve the efficient degradation of pollutants in wastewater. The review aims to provide a waste disposal alternative for the alleviation of environmental pollution and the promotion of green chemistry as well as sustainable development.