bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Concurrent Strength and HIIT-Based Endurance Training on Physical Fitness in Trained Team Sports Players: A Systematic Review and Meta-Analysis
Authors: Kang Jian , Ye Zhijing , Yin Xinxing , Zhou Changjing , Gong Bo
Published: November 2022

Background: Concurrent strength and HIIT-based endurance training (CT) has merit in time-saving in team sports. However, the effect of CT on physical fitness remained equivocal. This meta-analysis aimed to determine whether CT would produce an interference effect on the development of physical fitness when compared to strength training (ST) or HIIT-based endurance training (HET) alone in trained team sports players. Methods: A total of 2478 studies from three databases were screened. 52 full texts were reviewed. Seven studies were finally included and then subgroups were used for quantitative analysis. Results: Compared to ST alone, CT had a significant effect on the development of maximal lower-body strength in trained team sports players (MD 4.20 kg, 95% CI 0.71–7.68, p = 0.02, I2 = 20%), but there was no significant difference between the groups on training adaptation in lower-body power (SMD 0.08, 95% CI −0.23–0.39, p = 0.62, I2 = 26%). Furthermore, a sub-group analysis based on the internal organization order of CT revealed that there was no statistically significant subgroup effect between CT and ST alone in all parameters. Conclusions: Well-designed CT regimens did not interfere with the development of physical fitness of trained team sports players.
Exclusive Breastfeeding and Childhood Morbidity: A Narrative Review
Authors: Hossain Saldana , Mihrshahi Seema
Published: November 2022

Globally, diarrheal diseases and acute respiratory infections are the leading causes of morbidity and mortality in children under 5 years old. The benefits of exclusive breastfeeding in reducing the risk of gastrointestinal and respiratory infections are well documented. Optimal breastfeeding may potentially save the lives of about 800,000 children in low-income settings. Despite the evidence, around 63% of infants from birth to 6 months are not exclusively breastfed worldwide. We searched the literature published between 2010 and 2022 in Medline, Embase, and Scopus on the association between exclusive breastfeeding and infectious diseases. We selected and reviewed 70 relevant studies. Our findings expand and confirm the positive association between exclusive breastfeeding and reduced risk of a number of gastrointestinal, respiratory, and other infections in 60 out of 70 studies observed in both low- and high-income settings. Several studies analyzing exclusive breastfeeding duration reported that a longer exclusive breastfeeding duration is protective against many infectious diseases. This review also reported a lack of standardized definition for measuring exclusive breastfeeding in many studies. Overall, the results highlight the benefits of exclusive breastfeeding in many studies and suggests reporting exclusive breastfeeding in future studies using a consistent definition to enable better monitoring of exclusive breastfeeding rates.
Pollution Risk Assessment and Sources Analysis of Heavy Metal in Soil from Bamboo Shoots
Authors: Wang Zhihong , Chen Yingle , Wang Song , Yu Yujuan , Huang Wenyan , Xu Qiaolin , Zeng Lei
Published: November 2022

In order to investigate the pollution situation and sources analysis of heavy metals in bamboo shoot soil in Guangdong Province, a total of 175 soil samples were collected at 46 sites. Atomic fluorescence spectrophotometer and inductively coupled plasma mass spectrometry were used to determine the content of five heavy metals: lead (Pb), cadmium (Cd), arsenic (As), mercury (Hg), and chromium (Cr). In addition, the soil environmental quality was evaluated through different index methods, including single-factor pollution, Nemeiro comprehensive pollution, geoaccumulation, and potential ecological risk. Furthermore, the correlation coefficients were also discussed. The results showed that the soils collected were acidic or slight alkaline. The maximum content of Pb and As from some areas exceeded the standard limit value. The coefficient of variation value from six areas exceeded 100%. The index method mentioned above confirmed that the soil within study areas was divided into three pollution levels: no, slightly, and mild. Additionally, there was a very significant correlation between pH and Pb, Hg; the correlation between heavy metal As and Pb, Cr also reached a very significant level. The principal component analysis results show that PC1 accounts for 39.60% of the total variance, which includes Pb, Cd, and As. PC2 mainly includes Hg and Cr.
Factors Influencing Cortisol Concentrations in Breastmilk and Its Associations with Breastmilk Composition and Infant Development in the First Six Months of Lactation
Authors: Zielinska-Pukos Monika , Bryś Joanna , Kucharz Natalia , Chrobak Agnieszka , Wesolowska Aleksandra , Grabowicz-Chądrzyńska Iwona , Hamulka Jadwiga
Published: November 2022

Previous studies provided contradictory results regarding the influence of maternal, seasonal, and infant factors on breastmilk cortisol, and its associations with breastmilk composition and infant development. This study aimed to assess breastmilk cortisol levels at the first, third, and sixth months of lactation and evaluate the associations with maternal psychosocial, seasonal, and infant factors, breastmilk composition, and infant anthropometric and psychomotor development and temperament. Cortisol concentrations were assessed by ELISA in 24 h breastmilk samples obtained from 38 healthy mothers. Maternal psychological status was assessed by EPDS and PSS-10 and infant psychomotor development was assessed using the Children’s Development Scale (DSR). Breastmilk cortisol was 11.2 ± 6.2, 11.2 ± 4.3, and 12.7 ± 6.2 ng/mL at the first, third, and sixth months of lactation (p > 0.05), respectively. In the spring-summer season, we observed lower and higher levels of cortisol in the first and sixth months of lactation (p ≤ 0.05), respectively, but no other associations were detected regarding maternal or infant characteristics. In the third month of lactation, cortisol was related to breastmilk crude protein (β = 0.318, 0.007–0.630) and infant BMI z-score before adjustment for infant birthweight and sex (Model 2: β = 0.359, 0.021–0.697), but no other associations with breastmilk composition, infant development, or temperament were confirmed. Our results indicated that breastmilk cortisol is unrelated to maternal and infant factors and has limited influence on breastmilk crude protein, but not on infant anthropometric and psychomotor development.
Integrated Pangenome Analysis and Pharmacophore Modeling Revealed Potential Novel Inhibitors against Enterobacter xiangfangensis
Authors: Almuhayawi Mohammed S. , Al Jaouni Soad K. , Selim Samy , Alkhalifah Dalal Hussien M. , Marc Romina Alina , Aslam Sidra , Poczai Peter
Published: November 2022

Enterobacter xiangfangensis is a novel, multidrug-resistant pathogen belonging to the Enterobacter genus and has the ability to acquire resistance to multiple antibiotic classes. However, there is currently no registered E. xiangfangensis drug on the market that has been shown to be effective. Hence, there is an urgent need to identify novel therapeutic targets and effective treatments for E. xiangfangensis. In the current study, a bacterial pan genome analysis and subtractive proteomics approach was employed to the core proteomes of six strains of E. xiangfangensis using several bioinformatic tools, software, and servers. However, 2611 nonredundant proteins were predicted from the 21,720 core proteins of core proteome. Out of 2611 nonredundant proteins, 372 were obtained from Geptop2.0 as essential proteins. After the subtractive proteomics and subcellular localization analysis, only 133 proteins were found in cytoplasm. All cytoplasmic proteins were examined using BLASTp against the virulence factor database, which classifies 20 therapeutic targets as virulent. Out of these 20, 3 cytoplasmic proteins: ferric iron uptake transcriptional regulator (FUR), UDP-2,3diacylglucosamine diphosphatase (UDP), and lipid-A-disaccharide synthase (lpxB) were chosen as potential drug targets. These drug targets are important for bacterial survival, virulence, and growth and could be used as therapeutic targets. More than 2500 plant chemicals were used to molecularly dock these proteins. Furthermore, the lowest-binding energetic docked compounds were found. The top five hit compounds, Adenine, Mollugin, Xanthohumol C, Sakuranetin, and Toosendanin demonstrated optimum binding against all three target proteins. Furthermore, molecular dynamics simulations and MM/GBSA analyses validated the stability of ligand–protein complexes and revealed that these compounds could serve as potential E. xiangfangensis replication inhibitors. Consequently, this study marks a significant step forward in the creation of new and powerful drugs against E. xiangfangensis. Future studies should validate these targets experimentally to prove their function in E. xiangfangensis survival and virulence.
Burden of Malaria in Sao Tome and Principe, 1990–2019: Findings from the Global Burden of Disease Study 2019
Authors: Wang Yuxin , Li Mingqiang , Guo Wenfeng , Deng Changsheng , Zou Guanyang , Song Jianping
Published: November 2022

Background: Malaria is a parasitic infection transmitted by mosquito vectors, commonly found in tropical regions, and characterized by high morbidity and mortality. It causes a heavy disease burden in Sao Tome and Principe (STP), an island country in West Africa which at one time had a high incidence of malaria. Objective: This study aims to analyze the trend of disease burden of malaria in STP. Methods: The crude and age-standardized incidence, mortality, and disability-adjusted life years (DALYs) rate data of malaria were extracted from GBD 2019. Joinpoint 4.9 software was used to calculate the annual percentage change (APC) and the average annual percentage change (AAPC), which were also used to indicate the change in disease burden by different stages. Results: In general, the age-standardized incidence rate (ASIR), age-standardized mortality rate (ASMR), and age-standardized DALYs rate (ASDR) of malaria presented a decreasing trend between 1990 and 2019, with an average annual decrease of 5.6%, 6.2%, and 10.7%, respectively, in STP. Specifically, all indicators first presented an increasing trend from 1990 to about 2000, followed by a decreasing trend until 2019, although the incidence rebounded slightly after 2015. Overall, the ASIR, ASMR, and ASDR of malaria reduced by 77.08%, 87.84%, and 82.21%, respectively, in 2019 as compared to 1990. No significant differences in disease burden were found between males and females between 2005 and 2019. Children who were under 5 years old showed a relatively small decrease in the rate of DALYs as compared to other age groups, but remained the group with the highest disease burden of malaria in the country. Conclusions: The disease burden of malaria in STP showed a significant decrease between 1990 and 2019, but it will still be challenging to achieve the goal of eliminating malaria by 2025. The government and relevant authorities should aim to strengthen the prevention and surveillance of malaria and tailor population-specific interventions in order to reduce the disease burden of malaria in STP.
The Autonomous Capacity of the Elderly Population in Spain for Shopping and Preparing Meals
Authors: Pons-Novell Jordi , Guillen Montserrat
Published: November 2022

A loss of the ability to buy and prepare meals, especially in people aged 65 and over, leads to a deterioration in their optimal level of nutrition. The Index of Autonomy in Food Acquisition (IAFA) was used to identify contributing factors. This is a composite indicator for shopping and meal preparation that can be used to assess the degree of autonomous capacity observed in a specific group. Data from the European Health Survey in Spain (7167 respondents aged 65 and over) show that capacity decreased with age and that women were less affected than men, with very little difference found in levels of autonomous capacity by territory. However, in relation to different income levels, after standardizing for age and sex, no evidence was found for differences in the ability to access and prepare meals in groups that were separated by income level. This result shows the importance of standardizing when analysing food acquisition autonomy in groups of people aged 65 years and over.
The Influence of Point Cloud Accuracy from Image Matching on Automatic Preparation of Training Datasets for Object Detection in UAV Images
Authors: Zachar Paulina , Ostrowski Wojciech , Płatek-Żak Anna , Kurczyński Zdzisław
Published: November 2022

The dynamic development of deep learning methods in recent years has prompted the widespread application of these algorithms in the field of photogrammetry and remote sensing, especially in the areas of image recognition, classification, and object detection. Still, one of the biggest challenges in this field is the low availability of training datasets, especially regarding applications of oblique aerial imagery and UAV data. The process of acquiring such databases is labor-intensive. The solution to the problem of the unavailability of datasets and the need for manual annotation is to automate the process of generating annotations for images. One such approach is used in the following work. The proposed methodology for semi-automating the creation of training datasets was applied to detect objects on nadir and oblique images acquired from UAV. The methodology includes the following steps: (1) the generation of a dense 3D point cloud by two different methods: UAV photogrammetry and TLS (terrestrial laser scanning); (2) data processing, including clipping to objects and filtering of point clouds; (3) the projection of cloud points onto aerial images; and (4) the generation of bounding boxes bounding the objects of interest. In addition, the experiments performed are designed to test the accuracy and quality of the training datasets acquired in the proposed way. The effect of the accuracy of the point cloud extracted from dense UAV image matching on the resulting bounding boxes extracted by the proposed method was evaluated.
Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell Entry
Authors: Huang Shiqi , Taylor Carla G. , Zahradka Peter
Published: November 2022

Angiotensin-converting enzyme 2 (ACE2) is a target of interest for both COVID-19 and cardiovascular disease management. Even though lower ACE2 levels may be beneficial in SARS-CoV-2 infectivity, maintaining the ACE1/ACE2 balance is also crucial for cardiovascular health. So far, reports describing conditions capable of altering ACE2 protein levels, especially via dietary components, are limited. In this study, the effects of omega-3 polyunsaturated fatty acids (n3-PUFA) on the protein levels of ACE1 and ACE2 in rodent tissues, human endothelial and kidney cell lines, and human plasma were examined. The ability of n3-PUFA to affect the entry of the SARS-CoV-2 pseudovirus into cells was also tested. Docosahexaenoic acid (DHA), and in some cases eicosapentaenoic acid (EPA), but not α-linoleic acid (ALA), reduced both ACE1 and ACE2 (non-glycosylated p100 and glycosylated p130 forms) in the heart, aorta, and kidneys of obese rats, as well as in human EA.hy926 endothelial and HEK293 kidney cells. Dietary supplementation with either DHA or ALA had no effect on plasma soluble ACE2 levels in humans. However, treatment of HEK293 cells with 80 and 125 µM DHA for 16 h inhibited the entry of the SARS-CoV-2 pseudovirus. These results strongly suggest that DHA treatment may reduce the ability of SARS-CoV-2 to infect cells via a mechanism involving a decrease in the absolute level of ACE2 protein as well as its glycosylation. Our findings warrant further evaluation of long-chain n3-PUFA supplements as a novel option for restricting SARS-CoV-2 infectivity in the general population.
Pathophysiology and Therapeutic Approaches for Spinal Cord Injury
Authors: Lima Rui , Monteiro Andreia , Salgado António J. , Monteiro Susana , Silva Nuno A.
Published: November 2022

Spinal cord injury (SCI) is a disabling condition that disrupts motor, sensory, and autonomic functions. Despite extensive research in the last decades, SCI continues to be a global health priority affecting thousands of individuals every year. The lack of effective therapeutic strategies for patients with SCI reflects its complex pathophysiology that leads to the point of no return in its function repair and regeneration capacity. Recently, however, several studies started to uncover the intricate network of mechanisms involved in SCI leading to the development of new therapeutic approaches. In this work, we present a detailed description of the physiology and anatomy of the spinal cord and the pathophysiology of SCI. Additionally, we provide an overview of different molecular strategies that demonstrate promising potential in the modulation of the secondary injury events that promote neuroprotection or neuroregeneration. We also briefly discuss other emerging therapies, including cell-based therapies, biomaterials, and epidural electric stimulation. A successful therapy might target different pathologic events to control the progression of secondary damage of SCI and promote regeneration leading to functional recovery.
Anticarcinogenic Effects of Isothiocyanates on Hepatocellular Carcinoma
Authors: Zhang Yuting , Huang Huiting , Jin Libo , Lin Sue
Published: November 2022

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, accounting for about 90% of cases. Sorafenib, lenvatinib, and the combination of atezolizumab and bevacizumab are considered first-line treatments for advanced HCC. However, clinical application of these drugs has also caused some adverse reactions such as hypertension, elevated aspartate aminotransferases, and proteinuria. At present, natural products and their derivatives have drawn more and more attention due to less side effects as cancer treatments. Isothiocyanates (ITCs) are one type of hydrolysis products from glucosinolates (GLSs), secondary plant metabolites found exclusively in cruciferous vegetables. Accumulating evidence from encouraging in vitro and in vivo animal models has demonstrated that ITCs have multiple biological activities, especially their potentially health-promoting activities (antibacterial, antioxidant, and anticarcinogenic effects). In this review, we aim to comprehensively summarize the chemopreventive, anticancer, and chemosensitizative effects of ITCs on HCC, and explain the underlying molecular mechanisms.
A Common Target of Nitrite and Nitric Oxide for Respiration Inhibition in Bacteria
Authors: Wang Wei , Wang Jiahao , Feng Xue , Gao Haichun
Published: November 2022

Nitrite and nitric oxide (NO) are well-known bacteriostatic agents with similar biochemical properties. However, many studies have demonstrated that inhibition of bacterial growth by nitrite is independent of NO. Here, with Shewanella oneidensis as the research model because of its unusually high cytochrome (cyt) c content, we identify a common mechanism by which nitrite and NO compromise cyt c biosynthesis in bacteria, and thereby inhibit respiration. This is achieved by eliminating the inference of the cyclic adenosine monophosphate-catabolite repression protein (cAMP-Crp), a primary regulatory system that controls the cyt c content and whose activity is subjected to the repression of nitrite. Both nitrite and NO impair the CcmE of multiple bacteria, an essential heme chaperone of the System I cyt c biosynthesis apparatus. Given that bacterial targets of nitrite and NO differ enormously and vary even in the same genus, these observations underscore the importance of cyt c biosynthesis for the antimicrobial actions of nitrite and NO.
Synthesis and Antimicrobial Activity of Short Analogues of the Marine Antimicrobial Peptide Turgencin A: Effects of SAR Optimizations, Cys-Cys Cyclization and Lipopeptide Modifications
Authors: Dey Hymonti , Simonovic Danijela , Norberg-Schulz Hagen Ingrid , Vasskog Terje , Fredheim Elizabeth G. Aarag , Blencke Hans-Matti , Anderssen Trude , Strøm Morten B. , Haug Tor
Published: November 2022

We have synthesised short analogues of the marine antimicrobial peptide Turgencin A from the colonial Arctic ascidian Synoicum turgens. In this study, we focused on a central, cationic 12-residue Cys-Cys loop region within the sequence. Modified (tryptophan- and arginine-enriched) linear peptides were compared with Cys-Cys cyclic derivatives, and both linear and Cys-cyclic peptides were N-terminally acylated with octanoic acid (C8), decanoic acid (C10) or dodecanoic acid (C12). The highest antimicrobial potency was achieved by introducing dodecanoic acid to a cyclic Turgencin A analogue with low intrinsic hydrophobicity, and by introducing octanoic acid to a cyclic analogue displaying a higher intrinsic hydrophobicity. Among all tested synthetic Turgencin A lipopeptide analogues, the most promising candidates regarding both antimicrobial and haemolytic activity were C12-cTurg-1 and C8-cTurg-2. These optimized cyclic lipopeptides displayed minimum inhibitory concentrations of 4 µg/mL against Staphylococcus aureus, Escherichia coli and the fungus Rhodothorula sp. Mode of action studies on bacteria showed a rapid membrane disruption and bactericidal effect of the cyclic lipopeptides. Haemolytic activity against human erythrocytes was low, indicating favorable selective targeting of bacterial cells.
In Silico Study of the Acquired Resistance Caused by the Secondary Mutations of KRAS G12C Protein Using Long Time Molecular Dynamics Simulation and Markov State Model Analysis
Authors: Tu Gao , Liu Qing , Qiu Yue , Leung Elaine Lai-Han , Yao Xiaojun
Published: November 2022

Kirsten rat sarcoma viral oncogene homolog (KRAS) is a small GTPase protein which plays an important role in the treatment of KRAS mutant cancers. The FDA-approved AMG510 and MRTX849 (phase III clinical trials) are two potent KRASG12C-selective inhibitors that target KRAS G12C. However, the drug resistance caused by the second-site mutation in KRAS has emerged, and the mechanisms of drug resistance at atom level are still unclear. To clarify the mechanisms of drug resistance, we conducted long time molecular dynamics simulations (75 μs in total) to study the structural and energetic features of KRAS G12C and its four drug resistant variants to inhibitors. The combined binding free energy calculation and protein−ligand interaction fingerprint revealed that these second-site mutations indeed caused KRAS to produce different degrees of resistance to AMG510 and MRTX849. Furthermore, Markov State Models and 2D-free energy landscapes analysis revealed the difference in conformational changes of mutated KRAS bound with and without inhibitors. Furthermore, the comparative analysis of these systems showed that there were differences in their allosteric signal pathways. These findings provide the molecular mechanism of drug resistance, which helps to guide novel KRAS G12C inhibitor design to overcome drug resistance.
Protein Alterations in Cardiac Ischemia/Reperfusion Revealed by Spatial-Omics
Authors: Mezger Stephanie T. P. , Mingels Alma M. A. , Soulié Matthieu , Peutz-Kootstra Carine J. , Bekers Otto , Mulder Paul , Heeren Ron M. A. , Cillero-Pastor Berta
Published: November 2022

Myocardial infarction is the most common cause of death worldwide. An understanding of the alterations in protein pathways is needed in order to develop strategies that minimize myocardial damage. To identify the protein signature of cardiac ischemia/reperfusion (I/R) injury in rats, we combined, for the first time, protein matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and label-free proteomics on the same tissue section placed on a conductive slide. Wistar rats were subjected to I/R surgery and sacrificed after 24 h. Protein MALDI-MSI data revealed ischemia specific regions, and distinct profiles for the infarct core and border. Firstly, the infarct core, compared to histologically unaffected tissue, showed a significant downregulation of cardiac biomarkers, while an upregulation was seen for coagulation and immune response proteins. Interestingly, within the infarct tissue, alterations in the cytoskeleton reorganization and inflammation were found. This work demonstrates that a single tissue section can be used for protein-based spatial-omics, combining MALDI-MSI and label-free proteomics. Our workflow offers a new methodology to investigate the mechanisms of cardiac I/R injury at the protein level for new strategies to minimize damage after MI.
Involvement of AMPKα and MAPK-ERK/-JNK Signals in Docetaxel-Induced Human Tongue Squamous Cell Carcinoma Cell Apoptosis
Authors: Su Chin-Chuan , Lin Jhe-Wei , Chang Kai-Yao , Wu Cheng-Tien , Liu Shing-Hwa , Chang Kai-Chih , Liu Jui-Ming , Lee Kuan-I , Fang Kai-Min , Chen Ya-Wen
Published: November 2022

Cancers of the oral cavity can develop in the anatomic area extending from the lip, gum, tongue, mouth, and to the palate. Histologically, about 85–90% of oral cavity cancers are of the type squamous cells carcinomas (SCCs). The incidence of oral tongue SCC is higher in the tongue than any other anatomic area of the oral cavity. Here, we investigated the therapeutic effects and molecular mechanisms of docetaxel, which is a paclitaxel antitumor agent, on the cell growth of a human tongue SCC-derived SAS cell line. The results showed that docetaxel (10–300 nM) induced cytotoxicity and caspase-3 activity in SAS cells. Moreover, docetaxel (100 nM) promoted the expression of apoptosis-related signaling molecules, including the cleavages of caspase-3, caspase-7, and poly (ADP-ribose) polymerase (PARP). In mitochondria, docetaxel (100 nM) decreased the mitochondrial membrane potential (MMP) and Bcl-2 mRNA and protein expression and increased cytosolic cytochrome c protein expression and Bax mRNA and protein expression. In terms of mitogen-activated protein kinase (MAPK) and adenosine monophosphate-activated protein kinase (AMPK) signaling, docetaxel increased the expression of phosphorylated (p)-extracellular signal-regulated kinase (ERK), p-c-Jun N-terminal kinase (JNK), and p-AMPKα protein expression but not p-p38 protein expression. Moreover, the increase in caspase-3/-7 activity and Bax protein expression and decreased Bcl-2 protein expression and MMP depolarization observed in docetaxel-treated SAS cells could be reversed by treatment with either SP600125 (a JNK inhibitor), PD98059 (an MEK1/2 (mitogen-activated protein kinase kinase 1/2) inhibitor), or compound c (an AMPK inhibitor). The docetaxel-induced increases in p-JNK, p-ERK, and p-AMPKα protein expression could also be reversed by treatment with either SP600125, PD98059, or compound c. These results indicate that docetaxel induces human tongue SCC cell apoptosis via interdependent MAPK-JNK, MAPK-ERK1/2, and AMPKα signaling pathways. Our results show that docetaxel could possibly exert a potent pharmacological effect on human oral tongue SCC cell growth.
Development of a Molecular Aptamer Beacon Applied to Magnetic-Assisted RNA Extraction for Detection of Dengue and Zika Viruses Using Clinical Samples
Authors: da Silva Amanda Gabrielle , Goulart Luiz Ricardo , Löffler Philipp , Code Christian , Neves Adriana Freitas
Published: November 2022

Limitations in the detection of cocirculating flaviviruses such as Dengue and Zika lead us to propose the use of aptameric capture of the viral RNA in combination with RT-PCR (APTA-RT-PCR). Aptamers were obtained via SELEX and next-generation sequencing, followed by colorimetric and fluorescent characterizations. An APTA-RT-PCR assay was developed, optimized, and tested against the viral RNAs in 108 serum samples. After selection, sequence APTAZC10 was designed as a bifunctional molecular beacon (APTAZC10-MB), exhibiting affinity for the viral targets. APTA-RT-PCR was able to detect Dengue and Zika RNA in 43% and 8% of samples, respectively. Our results indicate that APTAZC10-MB and APTA-RT-PCR will be useful to improve the detection of Dengue and Zika viruses in a fast molecular assay for the improvement of infectious disease surveillance.
Improving Reporter Gene Assay Methodology for Evaluating the Ability of Compounds to Restore P53 Activity
Authors: Han Xinle , Du Jun , Shi Dandan , Li Lingjie , Li Dandan , Zhang Kun , Lin Suwen , Zhu Jingzhong , Huang Zoufang , Zhou You , Fang Zhengyu
Published: November 2022

Tumor suppressor protein P53 induces cycle arrest and apoptosis by mediating the transcriptional expression of its target genes. Mutations causing conformational abnormalities and post-translational modifications that promote degradation are the main reasons for the loss of P53 function in tumor cells. Reporter gene assays that can scientifically reflect the biological function can help discover the mechanism and therapeutic strategies that restore P53 function. In the reporter gene system of this work, tetracycline-inducible expression of wild-type P53 was used to provide a fully activated state as a 100% activity reference for the objective measurement of biological function. It was confirmed by RT-qPCR, cell viability assay, immunofluorescence, and Western blot analysis that the above-mentioned reporter gene system could correctly reflect the differences in biological activity between the wild-type and mutants. After that, the system was tentatively used for related mechanism research and compound activity evaluation. Through the tetracycline-induced co-expression of wild-type P53 and mutant P53 in exact proportion, it was observed that the response modes of typical transcriptional response elements (TREs) to dominant negative P53 mutation effect were not exactly the same. Compared to the relative multiple-to-solvent control, the activity percentage relative to the 100% activity reference of wild-type P53 can better reflect the actual influence of the so-called P53 mutant reactivator. Similarly, relative to the 100% activity reference, it can objectively reflect the biological effects caused by the inhibitor of P53 negative factors, such as MDM2. In conclusion, this study provides a 100% activity reference and a reliable calculation model for relevant basic research and drug development.
Crosslinked Structure of Polyacrylic Acid Affects Pulmonary Fibrogenicity in Rats
Authors: Tomonaga Taisuke , Nishida Chinatsu , Izumi Hiroto , Kawai Naoki , Wang Ke-Yong , Higashi Hidenori , Takeshita Jun-Ichi , Ono Ryohei , Sumiya Kazuki , Fujii Shota , Hata Yuki , Sakurai Kazuo , Morimoto Toshiki , Higashi Yasuyuki , Yamasaki Kei , Yatera Kazuhiro , Morimoto Yasuo
Published: November 2022

We conducted intratracheal instillations of polyacrylic acid (PAA) with crosslinking and non-crosslinking into rats in order to examine what kinds of physicochemical characteristics of acrylic-acid-based polymers affect responses in the lung. F344 rats were intratracheally exposed to similar molecular weights of crosslinked PAA (CL-PAA) (degree of crosslinking: ~0.1%) and non-crosslinked PAA (Non-CL-PAA) at low and high doses. Rats were sacrificed at 3 days, 1 week, 1 month, 3 months, and 6 months post-exposure. Both PAAs caused increases in neutrophil influx, cytokine-induced neutrophil chemoattractants (CINC) in the bronchoalveolar lavage fluid (BALF), and heme oxygenase-1 (HO-1) in the lung tissue from 3 days to 6 months following instillation. The release of lactate dehydrogenase (LDH) activity in the BALF was higher in the CL-PAA-exposed groups. Histopathological findings of the lungs demonstrated that the extensive fibrotic changes caused by CL-PAA were also greater than those in exposure to the Non-CL- PAA during the observation period. CL-PAA has more fibrogenicity of the lung, suggesting that crosslinking may be one of the physicochemical characteristic factors of PAA-induced lung disorder.
Proteomics of Salt Gland–Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion
Authors: Lu Chaoxia , Zhang Yuanyuan , Mi Ping , Guo Xueying , Wen Yixuan , Han Guoliang , Wang Baoshan
Published: November 2022

Soil salinization is one of the major factors restricting crop growth and agricultural production worldwide. Recretohalophytes have developed unique epidermal structures in their aboveground tissues, such as salt glands or salt bladders, to secrete excess salt out of the plant body as a protective mechanism from ion damage. Three hypotheses were proposed to explain how salt glands secrete salts: the osmotic hypothesis, a hypothesis similar to animal fluid transport, and vesicle-mediated exocytosis. However, there is no direct evidence to show whether the salt gland–secreted liquid contains landmark proteins or peptides which would elucidate the salt secretion mechanism. In this study, we collected the secreted liquid of salt glands from Limonium bicolor, followed by extraction and identification of its constituent proteins and peptides by SDS-PAGE and mass spectrometry. We detected 214 proteins and 440 polypeptides in the salt gland-secreted droplets of plants grown under control conditions. Unexpectedly, the proportion of energy metabolism-related proteins increased significantly though only 16 proteins and 35 polypeptides in the droplets of salt-treated plants were detected. In addition, vesicle transport proteins such as the Golgi marker enzyme glycosyltransferase were present in the secreted sap of salt glands from both control and salt-treated plants. These results suggest that trans-Golgi network-mediated vesicular transport and energy production contributes to salt secretion in salt glands.
Gaseous Detectors for Field Applications: Quality Control, Thermal and Mechanical Stability
Authors: Gera Ádám , Nyitrai Gábor , Surányi Gergely , Hamar Gergő , Varga Dezső
Published: November 2022

A cosmic muon imaging system is essentially a particle tracking detector as known from experimental High Energy Physics. The Multiwire Proportional Chamber (MWPC) once revolutionized this field of science, and as such it is a viable choice as the core element of an imaging system. Long term construction and operation experience was gathered from a Japanese–Hungarian collaboration that gave rise to the MWPC-based Muon Observatory System (MMOS), and is being used in Japan at the Sakurajima volcano. The present paper attempts to draw conclusions on the thermal and mechanical limits of the system, based on controlled measurements and detailed simulations. High temperature behavior and effects of thermal cycling and conditioning are presented, which appear to consistently allow one to propose quality control criteria. Regarding mechanical stability, the relation between gluing quality (tensile strength) and expected stress from vibration (during transportation) determines the safety factor to avoid damages. Both of these are presented and quantified in the paper using a conservative and austere approach, with mechanical simulations validated with experimental modal testing data. One can conclude that mechanical stress during industrial standard air freight shipping conditions is nearly a factor of three below the calculated maximum stress.
Percutaneous Approach in Endovascular Aortic Procedures Using a Suture-Mediated Closure Device
Authors: Oikonomou Kyriakos , Kvataia Akaki , Pfister Karin , Zygouridou Evgenia , Betz Thomas , Schierling Wilma , Sachsamanis Georgios
Published: November 2022

Background: The purpose of this study is to assess the efficacy of a suture-mediated closure device during percutaneous endovascular aortic repair. Methods: A single-center, retrospective analysis of patients undergoing endovascular repair for infrarenal, thoracic and thoracoabdominal aortic aneurysms and aortic dissections via percutaneous femoral access between April 2017 and June 2021 was performed. The primary endpoint of the study was the efficacy and technical success of the Perclose ProGlide closure device during percutaneous endovascular procedures. The secondary endpoints were intraoperative and postoperative inguinal and vascular complications during and after device use. Results: A total of 376 punctures were performed in 263 patients with the deployment of the ProGlide vascular closure system. Twenty-two cases involved percutaneous re-puncture as part of a staged procedure. The primary and secondary technical success rates were 93.1% (350/376) and 94.7% (356/376), respectively. In 20 patients (5.3%), intraoperative femoral exposure due to complications was required. Postoperative complications occurred in 13 cases (3.5%), 2 of which required surgical reintervention. There was no statistical significance between the type of endovascular procedure and primary technical success (p = 0.56). The introduction of larger-diameter sheaths was not associated with increased intraoperative and postoperative complication rates (p = 0.75 and p = 0.78, respectively). Percutaneous re-puncture of the vascular access site did not result in a lower overall technical success rate (20/22, 90.9% primary technical success rate, p = 0.67; 21/22, 95.5% secondary technical success rate, p = 0.86) or an increased number of perioperative complications (1/22, 4.5% intraoperative complications, p = 0.86; 2/22, 9.1% postoperative complications, p = 0.13). Conclusion: The application of the ProGlide closure system is a safe and efficient method to achieve hemostasis during percutaneous endovascular aortic repair. Complex aortic pathologies, which often require a staged approach with re-puncture, can also be successfully treated with this closure system.
Clinical and Multimodal Imaging Features of Choroidal Nevi in the Korean Population
Authors: Lee Chul Hee , Lee Hansang , Lee Seung Min , Choi Eun Young , Lee Junwon , Kim Min
Published: November 2022

Choroidal nevus is a precursor of choroidal melanoma. Multimodal imaging has become vital in predicting the malignant transformation of choroidal nevi. This single-center, retrospective study analyzed clinical characteristics and multimodal imaging findings of 168 choroidal nevi (164 patients) of the Korean population. The mean age at presentation was 50 ± 15 (range, 13–85) (women, n = 88 [53.7%]). Choroidal nevi (melanotic, n = 164 [97.6%]; postequatorial, n = 160 [95.2%]) were densely located between the optic disc and foveola (65.5%). The mean maximum linear basal diameter on fundus photography and thickness on optical coherence tomography were 2.97 ± 1.51 mm and 521 ± 297 μm, respectively. On ultrasonography, the mean thickness was 0.87 ± 0.60 mm. Choroidal nevi in women were associated with a higher maximum linear basal diameter (3.23 ± 1.65 vs. 2.68 ± 1.21 mm; p = 0.033) and age at diagnosis (52 ± 14 vs. 47 ± 16 years; p = 0.046) than those in men. Choroidal nevi with associated subretinal fluid (23.2%) presented with larger basal diameter (p = 0.049) and thickness on B-scan and optical coherence tomography (p < 0.001), but a younger age at diagnosis (p < 0.001) than those of dry nevi. This multimodal imaging study of choroidal nevi revealed some distinct characteristics, including topographic distribution, sex-related differences, and a younger age at diagnosis of nevi with fluid.
Editorial for the Special Issue: “Advances in Postoperative Pain Management and Chronic Postoperative Pain”
Authors: Cascella Marco
Published: November 2022

Acute and chronic pain are two completely distinct universes [...]
Plasmapheresis for a Patient with Catatonia and Systemic Lupus Erythematosus: A Case Report and Literature Review
Authors: Tsai Pei-Shan , Chen Yu , Chen Shou-Yen , Hsu Chung-Yuan , Wu Jiao-En , Lee Chih-Chun , Chan Tien-Ming
Published: November 2022

Neuropsychiatric systemic lupus erythematous (NPSLE) encompasses various psychiatric and neurological manifestations that develop in patients with systemic lupus erythematous (SLE), secondary to the involvement of the central nervous system (CNS). Although neuropsychiatric manifestations are commonly described in NPSLE, catatonia has been less frequently reported in patients with SLE. The roles of benzodiazepines (BZDs), immunosuppression, therapeutic plasma exchange (TPE), and electroconvulsive therapy (ECT) have all been reported in the management of catatonia. Furthermore, another research reported that catatonic symptoms associated with NPSLE were considerably improved by TPE. We, herein, report a case of catatonia in a patient with newly diagnosed NPSLE who exhibited a favorable prognosis through the early initiation of systemic immunosuppressants and TPE. Furthermore, we have reviewed the literature on the role of medication and plasmapheresis (PP), or TPE, in the treatment of catatonia that is associated with SLE.
Bacterial Aspiration Pneumonia in Generalized Convulsive Status Epilepticus: Incidence, Associated Factors and Outcome
Authors: Tortuyaux Romain , Wallet Frédéric , Derambure Philippe , Nseir Saad
Published: November 2022

Suspicion of bacterial aspiration pneumonia (BAP) is frequent during generalized convulsive status epilepticus (GCSE). Early identification of BAP is required in order to avoid useless antibiotic therapy. In this retrospective monocentric study, we aimed to determine the incidence of aspiration syndrome and BAP in GCSE requiring mechanical ventilation (MV) and factors associated with the occurrence of BAP. Patients were older than 18 years and had GCSE requiring MV. To distinguish BAP from pneumonitis, tracheal aspirate and quantitative microbiological criterion were used. Out of 226 consecutive patients, 103 patients (46%) had an aspiration syndrome, including 54 (52%) with a BAP. Staphylococcus aureus represented 33% of bacterial strains. No relevant baseline characteristics differed, including serum levels of CRP, PCT, and albumin. The median duration of treatment for BAP was 7 days (5–7). Patients with BAP did not have a longer duration of MV (p = 0.18) and ICU stay (p = 0.18) than those with pneumonitis. At 3 months, 24 patients (44%) with BAP and 10 (27%) with pneumonitis had a poor functional outcome (p = 0.06). In conclusion, among patients with GCSE, half of the patients had an aspiration syndrome and one-quarter suffered from BAP. Clinical characteristics and biomarkers were not useful for differentiating BAP from pneumonitis. These results highlight the need for a method to rapidly differentiate BAP from pneumonitis, such as polymerase-chain-reaction-based techniques.
Recent 137Cs Distribution in the Aegean Sea, Greece
Authors: Leivadaros Petros , Tsabaris Christos , Patiris Dionisis L. , Eleftheriou Georgios , Pappa Filothei K. , Androulakaki Efrossyni , Dasenakis Manos , Krasakopoulou Evangelia , Zervakis Vassilis
Published: November 2022

Recent spatial and vertical distributions of 137Cs activity concentration in the Aegean Sea are presented almost 30 years after the Chernobyl accident. The study aims to provide the current radioactivity levels of 137Cs in the Aegean Sea and to combine the 137Cs activity concentration with typical oceanographic parameters (T, S) in order to utilize them as tracers to identify/validate the different water masses that are present in the Aegean Sea. This work was performed in the frame of the “KRIPIS” project in 2017 for continuous investigations of the deep basins from all over the Aegean Sea and includes samplings from the water column layers of seven stations. The 137Cs activity concentrations were determined via lab-based gamma ray spectroscopy after appropriate chemical pre-concentration of 137Cs, while the salinity and temperature of the water column were obtained by in-situ measurements. The activity concentration values of 137Cs varied from 1.6 to 5.5 Bq m−3. Clear distinction of the Black Sea and Levantine Waters was obtained based on the combination of temperature and salinity values with 137Cs activity concentration. Furthermore, including 137Cs as a supplementary tracer, the Transitional Subsurface Aegean Waters were identified at the Myrtoan and Antikythera Straits, combining the salinity, temperature and 137Cs activity concentration.
Impacts of Emissions Trading Scheme Initiatives on Corporate Carbon Proactivity and Financial Performance
Authors: Zha Guiliang , Li Yongqing , Tang Qingliang
Published: November 2022

This study introduces the concept of carbon proactivity and considers not only the quantity of emissions but also corporate carbon-reduction efforts and actions to explore the relationship between carbon proactivity, the emissions trading scheme (ETS) mechanism, and corporate financial performance. A matched-pair approach was adopted to explore the difference in carbon proactivity between ETS and non-ETS firms. The study aims to investigate the impacts of an ETS on corporate carbon proactivity and whether participating in an ETS can help a firm achieve a desired outcome in which it can improve both environmental and economic performance. Using manually collected data on carbon disclosure, it was found that carbon proactivity is higher among firms that participate in an ETS than among those that do not, and carbon proactivity is trending upward for the participating firms. In addition, evidence suggests that while investing more resources in carbon proactivity decreases current financial performance, it will boost future financial performance. This relationship is observed among firms that participate in an ETS. This study extends the understanding of the relationship between ETSs, corporate carbon proactivity, and corporate financial performance. It also provides evidence on how to improve the ETS mechanism.
Spatial Evolution of an Oil City: A Case Study of Karamay, Northwest China
Authors: Peng Yifei , Fujiyama Mamiko , Ishida Toshikazu
Published: November 2022

This study investigates how Karamay, a typical mining city in Northwest China, has expanded and evolved over the last three decades (1994–2021) with a special study area of the oil production “townships” which fully expressed the characteristics of the scattered spatial pattern of oil cities, by using remote sensing (RS) images and geographical information system (GIS) spatial analyses. The expansion rate and urbanization development index, spatial orientation, and urban compactness are used to discuss the expansion features. The results indicate that Karamay city has continued to expand in the past 30 years, and there were two stages of urban expansion regarding expansion orientation. During the expansion, there was a trend toward more urban compactness. Karamay’s urban space has gradually evolved from the original scattered distribution of townships to functionally concentrated urban areas. Socioeconomic factors and multilevel policies were the main factors influencing urban expansion. Suggested strategies for the future development of oil cities in China were given accordingly. Two main innovative points are presented in this paper. First, a unique perspective was given on spatial changes in oil townships to better capture how industrial activities influence the urban expansion of oil cities, which has not been found in other studies. Second, this study is the first to combine the urban expansion process with the changing of scattered spatial characteristics of cities, which has reference significance for the sustainable development of these types of cities and contributes to the diversity of case backgrounds for discussing the possibility of compact urban growth.
5-Chloro-6-oxo-6H-xantheno[4,3-d]thiazole-2-carbonitrile
Authors: Paraskevas Konstantinos , Iliopoulos-Tsoutsouvas Christos , Georgiou Eleftheria A. , Kostakis Ioannis K.
Published: November 2022

Xanthones and benzothiazoles are important classes of heterocyclic compounds with versatile biological activities. Herein, we describe a straightforward and scalable synthesis of 5-chloro-6-oxo-6H-xantheno[4,3-d]thiazole-2-carbonitrile, a thiazole-fused xanthone, via a six-step approach, using Appel’s salt for the synthesis of the thiazole ring. The thiazole-fused xanthone was fully characterized employing 1H and 13C NMR spectra, using direct and long-range heteronuclear correlation experiments (HMBC and HMQC).