bluuebunny/crossref_metadata_2025 huggingface dataset:
Exoplanets Around Red Giants: Distribution and Habitability
Authors: chen Ruixuan , Jiang Jonathan , Rosen Philip , Fahy Kristen , Chen Yanbei
Published: November 2022

As the search for exoplanets continues, more are being discovered orbiting Red Giant stars. We use current data from the NASA Exoplanet Archive to investigate planet distribution around Red Giant stars and their presence in the host’s habitable zone. As well, we update the power law relation between planet mass and stellar radius found in previous studies and provide more detailed investigations on this topic. Ten Red Giant-hosted exoplanets are found to be in the optimistically calculated habitable zone, while five of which are in the conservative habitable zone. We believe additional exoplanets can be found in habitable zones around Red Giants using the direct imaging method and more powerful detection instrumentation.
RNA-Seq reveals molecular strategies in response to chronic hypoxic challenge in the gills of Takifugu rubripes
Authors: Lu Yun , Shang Fengqin , Li Yan , Han Bing , Wei Renjie , Liu Shengmei , Liu Ying , Liu Yang , Wang Xiuli
Published: November 2022

Hypoxia negatively affects fish behavior, growth, physiological metabolism, and immune responses, or even death. Due to the high density of breeding, sudden power outages and hypoxia stresses have become a serious problem for the aquaculture of Takifugu rubripes. As fish gills are the first organ to be affected by hypoxia in the aquatic environment, the ability to sense and respond to hypoxic stress is crucial. At the molecular level, therefore, the regulation of gene expression in the gills is essential for hypoxia tolerance. Using an RNA-Seq approach in the Illumina sequencing platform, we investigated transcriptome modulation in the gills of T. rubripes, in response to chronic hypoxic stress (2.5 ± 0.5 mg/L for 10 days). Transcriptomic analysis showed that 143 differentially expressed genes (DEGs) (|log2foldchange| >=1 & p.adj < 0.05) were detected under chronic hypoxic exposure (84 up-regulated and 59 down-regulated genes). According to GO annotation, KEGG, and GSEA enrichment analysis, we found that chronic hypoxia can cause damage to animal tissues. However, the functional analysis also showed that T. rubripes can improve the ability to resist hypoxic stress by changing the expression of genes related to metabolic processes and O2 extraction. Of particular interest was the activated immune-related pathways, including Cytokine-cytokine receptor interaction and CCR chemokine receptor binding, which play an essential role in the adaptation of T. rubripes to chronic hypoxic stress. Therefore, the regulated genes and pathways identified in this study enrich the molecular regulatory mechanism of T. rubripes in different hypoxic conditions, which is valuable for further studies regarding hypoxia tolerance in T. rubripes and other teleost fish, and pave the way for future research to understand the tissue-specific molecular responses to hypoxic stress.
Spatio-temporal Study of Pollutants in the Rio De Ondas Hydrographic Basin, West of Bahia, Brazil Using Multivariate Analysis
Authors: Rego Enoc Lima , Portela Joelma Ferreira , Ribeiro Camila Lima , Souza João Pedro Rudrigues , Tonhá Myller de Souza , Silva José Domingos Santos , Souza Jurandir Rodrigues
Published: November 2022

Water bodies are containers that receive a large load of pollutants through the release of domestic, industrial, and agricultural effluents. With this focus, this work aimed to conduct a temporal-spatial variability study in the Rio de Ondas Hydrographic Basin through multivariate statistical analysis. For this, seventeen collection sites were established in four stations along the Rio de Ondas and its tributaries between 2017 and 2018. Ionic chromatography with suppressed conductivity was used for ions determination, while ICP-OES determined metals' total concentrations. The land use and occupation assessment between 1985 and 2015 was conducted using images from Landsat 5 and 8 satellites and the descriptive and multivariate analysis of the data using version 10 of the Statistica software. The results showed that, in thirty years, there was a growth of 569% of agricultural activities in the watershed area, with significant suppression of native vegetation, favoring the transport of pollutants to rivers. Ca2+, PO42−, Al, Cu, and Zn concentrations showed a statistically significant difference between the seasons, with higher medians in the rainy season. Rainy season influenced the formation of three groups in the PCA, consisting of electrical conductivity, salinity, TDS, and PO42− (group 1); temperature, Fe, SO42− and Cl− (group 2); and Ca2+, Mg2+, Na+, and HCO3− (group 3). The strong correlation between parameters of each group indicates anthropic influence on the watershed's water quality. However, levels are within the potability standard.
Self-care and Health-related Quality of Life among Heart Failure patients in Jimma Medical Center, Southwest Ethiopia
Authors: Seid Sheka Shemsi , Ibro Shemsedin Amme , Senbiro Ismael Ahmed , Ahmed Abdulwahid Awol
Published: November 2022

Background:Understanding self-care behaviour and its relationship with health-related quality of life (HRQoL) in patients with Heart failure help a healthcare provider to identify patients who still need but lack the self-care skills required to manage their disease condition at home. However, despite its growing importance and obvious relationships with positive health outcomes,the evidence to justify its popularity is not well documented. This study aimed to assess the extent of heart failure self-care and its relationship with the HRQoL among patients with heart failure.
Methods: An institutional-based descriptive correlational study was conducted on 176 heart failure patients at Jimma Medical Canter. The data were analyzed using SPSS version 26. Both descriptive and analytical statistical tests were utilized. A multivariate linear regression analysis was done to determine the relationship between self-care behaviour and HRQOL and different independent variables. Variables with a p-value< 0.05 were considered as significant predictors of the outcome variable.
Results:Of all participants, the majority, 93(52.8%) were males. The average self-care scores for maintenance, management, and confidence were 44.35 ±16.75, 43.81±19.15, and 50.16±24.17, respectively. None of the self-care dimensions reached the self-care adequacy cut point of 70. The majority of the study participants 111(63.07%) had poor quality of life. Significant linear associations were observed between self-care management and overall HRQoL (βs=-1.26, p=.000) and physical dimension (βs=0.881, p=.000). Self-care confidence score was also significantly associated with total HRQoL(βs=-.579, P= 0.029), physical(βs=.507, p=.007) and emotional dimensions score(βs=-.299, p=0.038).
Conclusions: The study participants had inadequate self-care behaviour and poor health-related quality of life. HF self-care was associated with better health-related quality of life. Therefore, the healthcare provider should identify patients with heart failure who lack self-care behaviours required to manage their condition at home and focus should also be given to specific educational interventions that target determinants of self-care behaviour and quality of life.
Proteomic analysis of circulating small extracellular vesicles unique to cervical cancer
Authors: Molika Piyatida , Leetanaporn Kittinun , Rungkamoltip Petchploy , Roytrakul Sittiruk , Hanpresertpong Jitti , Navakanitworakul Raphatphorn
Published: November 2022

Background Small extracellular vesicles (sEVs) are membrane vesicles released by healthy and malignant cells. sEVs are potential biomarkers for cancer diagnosis. Cervical cancer (CC) is the fourth most common cancer in females worldwide. Existing biomarkers, such as squamous cell carcinoma antigens, show low specificity. Hence, a novel biomarker for the diagnosis of CC is required. This study aimed to identify potential candidates in sEVs through proteomic analysis for the diagnosis of CC and to determine the EV protein profile to distinguish between healthy and CC serum samples. Methods The number and size distribution of sEVs in healthy controls (HC) and CC were measured using nanoparticle tracking analysis. Differential ultracentrifugation combined with size-exclusion chromatography was used to isolate and purify sEVs derived from the serum of HC and CC. The isolated sEVs were characterized using western blotting and transmission electron microscopy. Liquid chromatography-tandem mass spectrometry was used to identify and compare the protein profiles between CC and HC. EV proteins were validated using the TCGA database. Results The particle concentration in CC was marginally higher than that in HC. The mode size of the particles in CC was significantly smaller than that in the HC-derived particles. Proteomic and functional protein analyses revealed a difference in the EV protein profiles between HC and CC. We found three and 18 uniquely expressed proteins in HC and CC, respectively. Unique EV proteins in CC are involved in angiogenesis and the Ras, VEGF, and FAS signaling pathways, while EV proteins in HC are involved in cellular homeostasis. EV proteins such as C1QB, MYO3B, and NADSYN1 were significantly upregulated in CC and primary tumor tissues, whereas MAFK, OR13C9, PIK3C2, PLCB4, RAB12, and VIP were downregulated in CC sEVs and primary tumor tissues. Conclusion Our study provides useful insights into the potential of sEVs as noninvasive biomarkers for CC diagnosis. Validation with a well-designed cohort should be performed to assure the clinical diagnostic value of specific protein markers for CC sEVs.
Effects of Distinct Dual-tasks on Gait and the Correlation between Gait speed and Clinical Features in Parkinson’s disease
Authors: Zhang Meimei , Gan Yawen , Wang Xuemei , Wang Zhan , Zhang Yumei
Published: November 2022

Background Gait impairment is a common and disabling motor symptom in Parkinson’s disease (PD), deteriorated gait parameters have showed in both single-task (ST) and dual-task (DT) conditions. The aim of this study was to investigate the effects of different motor-cognitive and motor-motor DTs on gait and the correlation between gait speed and clinical features in PD patients.
Methods Fifty-six individuals with PD completed two motor-cognitive DTs (serial-7 subtractionand digit backward) and one motor-motor DT (button pressing). Spatiotemporal gait parameters were evaluated by wearable sensors. DT effects (DTEs) of gait parameters were calculated. Clinical variables recorded including Hoehn & Yahr (H-Y) staging, Movement Disorder Society Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) part I, II and III, New Freezing of Gait Questionnaire (NFOG-Q), Montreal Cognitive Assessment (MoCA), Hamilton Anxiety Rating Scale (HAMA), Hamilton Depression Rating Scale (HAMD), 39-item Parkinson’s Disease Questionnaire (PDQ-39) and Nonmotor Symptom Scale (NMSS).
Results Gait parameters including gait speed, cadence, stride length, gait cycle duration, double support phase deteriorated under the motor-cognitive DT conditions by Paired-sample t test and Wilcoxon signed-rank test (p＜0.01, p＜0.05). The motor-motor DT had no significant effect on gait performance except for gait speed (p＞0.05). The serial-7 subtraction DT paradigm had similar effect on gait with the digit backward DT. Gait speed was negatively correlated with MDS-UPDRS I, II, HAMA, HAMD, NMSS and PDQ-39 scores in PD patients under both ST and DT conditions (p＜0.01, p＜0.05).
Conclusion Effects of DT conditions on gait deficits were independent of the types of cognitive tasks. Gait speed was influenced by clinical features of PD under both ST and DT conditions. Whatever the types, motor-cognitive DT training should be used to improve gait performance under DT conditions, which is required to provide more therapeutic support of PD patients in the future.
Efficacy of futibatinib, an irreversible fibroblast growth factor receptor inhibitor, in FGFR-altered breast cancer
Authors: Meric-Bernstam Funda , Saridogan Turcin , Akcakanat Argun , Zhao Ming , Evans Kurt , Yuca Erkan , Scott Stephen , Kirby Bryce , Zheng Xiaofeng , Ha Min Jin , Chen Huiqin , Ng Patrick , DiPeri Tiimothy , Mills Gordon , Rodon Jordi , Damodaran Senthil
Published: November 2022

The role of the fibroblast growth factor receptor (FGFR) gene alterations as therapeutic targets in breast cancer have not been well characterized. Futibatinib (TAS-120; Taiho) is a novel pan-FGFR inhibitor. We sought to determine the efficacy of futibatinib in breast cancer models withFGFRalterations. Nine breast cancer patient–derived xenografts (PDXs) with a variety ofFGFR1-4 alterations and expression levels were treated with futibatinib. FGFR gene expression between patient tumors and matching PDXs was significantly correlated. Futibatinib inhibited tumor growth in 3 of 9 PDXs, with tumor stabilization in anFGFR2-amplified model and prolonged regression in an FGFR2 Y375C mutant/amplified model. FGFR2 overexpression and, to a greater extent, FGFR2 Y375C expression in MCF10A cells enhanced cell growth and sensitivity to futibatinib. Per institutional and public databases,FGFR2mutations and amplifications had a population frequency of 1.1–2.6% and 1.5–2.5%, respectively.FGFR2alterations in breast cancer may represent infrequent but highly promising targets for futibatinib.
Sleep duration and consumption of ultra-processed foods among Brazilian adolescents
Authors: Rocha Luana , Inácio Monique , Gratão Lucia , Miquelanti Isabella , Andaki Gustavo , Cunha Cristiane , Oliveira Tatiana , Pessoa Milene , Mendes Larissa
Published: November 2022

Background: Sleep duration influences adolescents eating patterns and quality of life. Thus, the study objective was to verify the association between sleep duration and ultra-processed food (UPF) intake among Brazilian adolescents participating in the Study of Cardiovascular Risks in Adolescents (ERICA).
Methods: Cross-sectional study using ERICA data, conducted between March 2013 and December 2014 with adolescents aged 12 to 17 years from public and private schools. UPF intake was obtained by recording a 24-hour food diary, and sleep duration through a questionnaire, stratified into: recommended sleep duration (from 8 to 10 hours) and inadequate sleep duration (less than 8 hours and more than 10 hours). Linear regression was performed to verify the association between sleep duration and UPF consumption.
Results: 66,791 adolescents were evaluated. Adolescents with inadequate sleep duration showed an increase in UPF consumption.
Conclusion: This result can contribute to the development of multifactorial interventions to improve adolescents’ quality of life, emphasizing the importance of adopting healthy lifestyle habits.
Validity and reliability of the Swedish versions of the HLS-EU-Q16 and HLS-EU-Q6 questionnaires
Authors: Bergman Lina , Nilsson Ulrica , Dahlberg Karuna , Jaensson Maria , Wångdahl Josefin
Published: November 2022

Background: In Europe at least one in every three people has low health literacy. The Health Literacy Survey European Questionnaire (HLS-EU-Q16) has been adapted and translated into Swedish but not psychometrically evaluated. The Swedish HLS-EU-Q6 has not been used in any study nor has it been validated. We aimed to study the psychometric properties of the Swedish versions of the HLS-EU-Q16 and HLS-EU-Q6.
Methods: A psychometric study of Swedish-speaking adults ≥ 18 years (n = 347). Statistical analysis was guided by The COnsensus-based Standards for the selection of health Measurement Instruments and included item distributional statistics, construct and criterion validity, test-retest reliability and internal consistency.
Results: Swedish HLS-EU-Q16: There were no floor effects; a ceiling effect was noted in 28% of respondents. Construct validity showed correlations with education level (rho = 0.135), self-perceived health (rho = 0.167), electronic health literacy (rho = 0.496), age (rho = 0.71), and Swedish HLS-EU-Q6 (rho = 0.840). Test-retest showed Cohen’s κ = 0.822. For internal consistency, Cronbach’s α = 0.89, and the split-half reliability = 0.93. Swedish HLS-EU-Q6: No floor or ceiling effects were noted. Construct validity showed correlations with education level (rho = 0.157), self-perceived health (rho = 0.177), age (rho = 0.400), electronic health literacy (rho = 0.551), and the Swedish HLS-EU-Q16 (rho = 0.840). The agreement between the Swedish HLS-EU-Q16 and Swedish HLS-EU-Q6 was poor, Cohen’s κ = 0.43. Test-retest showed Cohen’s κ = 0.812. For internal consistency, Cronbach’s α = 0.77, and the split-half reliability = 0.80.
Conclusions: The Swedish version of the HLS-EU-Q16 has acceptable psychometric properties, and based on the results we recommend its use to measure health literacy. Our results did not demonstrate the validity of the Swedish version of HLS-EU-Q6 because the agreement with the Swedish HLS-EU-Q16 was poor.
Cryptic colonization and transmission of mcr-1-positive Escherichia coli in mouse intestines
Authors: Shen Yingbo , Lv Ziquan , Chen Kun , Liu Weiwen , Xiang Qiumei , Jiang Junyao , Shao Dongyan , Liu Dejun , Zhang Rong , Wu Congming , Wang Yang , Ke Yuebin , Shen Jianzhong
Published: November 2022

Background Mobile colistin-resistance gene mcr-1 is prevalent among various bacteria, hosts and countries, especially in the guts of humans and animals. However, the biological basis for mcr-1 colonization and transmission in the intestines is largely unknown.
Methods We used mouse models to mimic exposure to mcr-1-positive Escherichia coli (MCRPEC, harboring an IncI2 plasmid positive for mcr-1) in human intestines without and with antibiotic pretreatment, respectively. We used cultivation and qPCR method to determine the presence and quantity of MCRPEC. We also used Fluorescence in situ hybridization (FISH) method to locate mcr-1-positive bacteria in situ and confocal laser scanning microscopy method to examine the interaction between MCRPEC and Caco-2 cell in vitro. Finally, we used mouse model to investigate the transmission of MCRPEC among individuals.
Results We found two approaches of MCRPEC colonization in the mouse intestines after exposure. In mice with intestinal microbiota homeostasis, MCRPEC was transient in the large intestines, while mcr-1 was detected at lower abundances (10-4–10-5) for at least 21 days as relic DNA or mcr-1-positive uncommon bacteria (MCRPUB). In mice with intestinal microbiota dysbiosis, MCRPEC colonized the intestines directly with a high shedding load (~108 CFU/g feces) and high mcr-1 abundance (~10-2). The transmission model confirmed that both MCRPEC and MCRPUB could cryptically transfer among individuals and persist for long periods.
Conclusions These results demonstrate two approaches of MCRPEC colonization and a long persistence of MCRPUB or an antetype in mouse intestines as well as their transmission among mice, which partially explains the widespread prevalence of mcr-1-positive IncI2 plasmid among hosts and its long persistence in the gut even without antibiotic pressure. Contaminated food enables exposure to pathogens that can colonize human intestines; thus, reducing MCRPEC/MCRPUB in livestock and animal-derived food and preventing MCRPEC/MCRPUB transmission in ecosystems under the "One Health" perspective are crucial.
Distribution, local use, and bio-prospecting opportunity of Ocimum americanum L around North Western part of Amhara Region, Ethiopia
Authors: Merawi Edeget , Birhanu Taye
Published: November 2022

Ocimum americanum encompasses various uses. The plant is claimed as a food additive for its aromatic effect on different foods and folk medicine for various complaints. However, the plant genetic resource use category, distribution, and associated community knowledge have not been properly and systematically documented and utilized. Accordingly, this survey was performed to explore the bio-prospecting opportunity of Ocimum americanum, and its associated community knowledge to provide baseline data for bio-digging companies. Purposive and proportional samplings were jointly used to select districts, villages, users, and growers who participated in the study. Dembiya, Gonder Zuria, Takusa, Alefa, Bahir Dar city, and Bahir Dar Zuria districts were selected based on the growth potential and user availability. Data was collected through semi-structured interviews, group discussions, and field observation. The community used Ocimum americanum for various purposes; for ailment treatment (31.67%), food flavor (26.6%), food preservative (7.7%), and to give aroma to different foods (8.33%). 31.67 % of the respondents replied as they are a user group of the plant for its ethno medicinal purpose whereas the remaining 68.34% of them claimed as they are not used for medical purposes. They are used to treat majorly for depression/headaches, and evil spirits. Concoction (21.05 %), cutting and inserting whole plant (15.79%), grinding (15.79%), melting (5.26%), Fumigation (10.53%), and smelling (31.58%) were used as methods of preparation and administration. Medicinal dosage is not quantized and varied up to the users based on the intensity and the severity of the disease. A total of nine typical basil-spiced traditional foods were listed. The highest fidelity level [a particular use category] of basil was food flavor (18.33%) followed by a similar result [18.33%] for Food flavor/preservative. The output of the direct matrix analysis showed Chilli pepper spice was the preferred local food sweetened by basil for its various purposes by the local people followed by hot-spiced pepper. Therefore, the present survey, inferred the community traditionally uses basil for its aromatic, preservative, flavor, and ailment treatment role. Therefore, novel genetic resources should be further researched and promoted for versatile use. It gives a clue for bio-prospecting companies working in the food and pharmacological industries to access the associated local knowledge and practices documented as well as the plant genetic resource to search for new biochemical compounds responsible for medicinal, preservative, and aromatic effects.
The Application of Rapid Response Team in Category 1 Emergency Caesarean Section Teaching for OBGYN Residents in the Delivery Room
Authors: Xu Xia , Lin Ying , Weng Ling , Guo Yanni , Lin Lin , Yan Jianying
Published: November 2022

Background
Category 1 caesarean section (CS) can be a life-saving procedure when there is immediate threat to the life of the woman or fetus. However, category 1 CS is an challenge for OBGYN residents, and it is necessary to establish a effective and straightforward teaching strategy. This study aimed to evaluate the efficiency of rapid response team (RRT) oncategory 1 CS teachingfor OBGYN residents in the delivery room.
Methods
A total of 142 residents who underwent standardized residency training programs in the delivery room were divided into a RRT teaching group and a traditional response (TR) teaching group. In the RRT teaching group, Category 1 emergency CS teaching was started and explored by rapid response team. The training included both theoretical and practical components. After the training, decision-to-delivery interval (DDI) , neonatal Apgar score , operation time and rate of postpartum hemorrhage (PPH) were compared. A questionnaire on the subjective assessment of various aspects of the program was conducted at the end of the training period.
Results
The decision-to-delivery internal (DDI) in minutes in the RRT teaching group (n=72) was significantly shorter than that of the TR teaching group (n=70) (11.83±4.16 vs 13.56±5.47, p = 0.0364). The score of satisfaction from residents in the RRT teaching group was significantly higher than that of the TR group [7 (6, 9) vs 9 (7, 10), p =0.0154 ). Compared wtih the TR teaching group , more residents thought their clinical skills have been improved (94.29% vs 100%, p=0.0396) and willing to recommend their training method to others (91.43% vs 100%, p=0.0399) in the RRT teaching group . However, no significant differences were observed in the incidence of postpartum hemorrhage between the two groups.
Conclusions
RRT teaching is beneficial in the standardized training and teaching of residents in the delivery room. It improves the DDI of category 1 emergency caesarean section and the degree of satisfaction.
Protected areas fail to cover the full annual cycle of migratory butterflies
Authors: Chowdhury Shawan , Cardillo Marcel , Chapman Jason , Green David , Norris Ryan , Riva Federico , Zalucki Myron , Fuller Richard
Published: November 2022

Effective conservation of migratory species relies on protection throughout their annual cycle1, something that has not been assessed globally for migratory insects. Here, we develop seasonal ecological niche models for 405 migratory butterfly species globally to assess whether they are adequately covered by protected areas (PAs) across their full annual cycle. PA coverage was inadequate in at least one season for 83% of migratory butterflies and 17% only achieved target representation in one season, and 43% did not meet the target representation in any season. While 95% of migratory butterflies met the target representation in Australia, only 1% did so in the Oriental region, indicating marked geographic variation in PA coverage. Coordinated efforts, across multiple countries, are needed to develop PAs that conserve migratory animals.
Dose-dependent proliferative and pro-oxidative effects of earthworm coelomic fluid on the HCT116 colon cancer cell line
Authors: Małota Karol , Urbisz Anna , Skonieczna Magdalena
Published: November 2022

Natural origin substances are an important source of medicinal compounds and have been used for centuries as remedies for many ailments. One source of these substances is earthworm (EW) coelomic fluid (CF). In this paper, we present the effects of earthworm Dendrobaena veneta CF on the HCT116, A549 and BEAS-2B cell lines. We used two fractions of CF, one heated to 70°C (CF-H) and unheated CF (CF-NH). Moreover, we used two different sets of doses: high doses (500, 250, 125, 62.5 µg/ml) and low doses (16, 8, and 4 µg/ml). Our results showed no cancer-selective cytotoxicity against any studied cell line in both CF-H and CF-NH groups. Interestingly, we discovered that low doses of CF-NH cause proliferative and pro-oxidative effects on the studied cell lines. These effects, to the best of our knowledge, are described here for the first time, and we believe it is an interesting new starting point in earthworm coelomic fluid studies.
Mixed Methods Evaluation of the Inaugural Year of the Cancer Prevention and Control Research Network’s (CPCRN) Scholars Program
Authors: Escoffery Cam , Petagna Courtney , Wangen Mary , Flicker Kimberly J. , Noblet Samuel B. , Sakhuja Mayank , Thomson Cynthia A. , Morrato Elaine H. , Adams Swann , Leeman Jennifer , Friedman Daniela B.
Published: November 2022

Purpose: A diverse workforce trained in dissemination & implementation (D&I) science is critical for improving cancer outcomes and reducing cancer-related health disparities. This study aims to describe and evaluate impact of the Cancer Prevention and Control Research Network (CPCRN) Scholars Program in preparing scholars for collaborative careers in cancer control and implementation research and practice, and offers evaluation-driven recommendations for program improvements.
Methods: The CPCRN Scholars Workgroup conducted a sequential, mixed methods evaluation. We collected baseline and follow-up surveys and invited all 20 scholars and 10 mentors to participate in an exit interview. We assessed the experience with the Scholar’s program, ratings of D&I competences, progress on their project, feedback about the curriculum, and understanding of implementation science.
Results: Over 86% partially or fully completed their project within 9 months; 78% of scholars engaged with a CPCRN workgroup. Scholars rated the following program components as valuable: the Putting Public Health Evidence in Action (PPHEIA) training (88.9%), D&I training modules (83.3%), and webinars (kickoff webinar-88.9% and selecting theories/models-88.9%). There was an increase in D&I competencies from baseline to posttest, with the greatest in community engagement topics. About 78% reported that they were satisfied with format of the activities and increased confidence in ability to discuss D&I concepts. From the qualitative interviews, the benefit of the program was becoming more knowledgeable about D&I research and networking.
Conclusion: The inaugural year of the program yielded positive results, particularly related to increasing knowledge about D&I science and cancer control. This program builds the capacity of students, researchers and practitioners in D&I science.
Evaluation of Glutathione S-transferase Mu 3 (GSTM3) levels in sperm as a simple method to predict oxidative DNA damage and seminogram alterations
Authors: Llavanera Marc , Ribas-Maynou Jordi , Mateo-Otero Yentel , Benet Jordi , Bonet Sergi , Yeste Marc
Published: November 2022

Background. Previous evidence suggested sperm Glutathione S-transferase Mu 3 (GSTM3) to be essential for an appropriate mitochondrial function, plasma membrane stability and oxidative regulation of mammalian sperm. In humans, however, neither has this enzyme been related to semen alterations nor has it been reported to be associated to oxidative DNA damage and (in)fertility.
Methods. The aim of the present study was to assess the utility of GSTM3 to predict spermiogram alterations and oxidative DNA damage in sperm. A total of 34 semen samples were collected, evaluated by conventional semen analysis, and subsequently cryopreserved. Frozen-thawed sperm samples were assessed for DNA fragmentation and the presence, localization and content of GSTM3.
Results. Sperm GSTM3 content was positively associated to sperm quality parameters (P < 0.05). Specifically, lower levels of this antioxidant enzyme were observed in asthenoteratozoospermic and oligoasthenoteratozoospermic men (P < 0.05), but not idiopathic infertile patients (P > 0.05), when compared to normospermic samples. Moreover, sperm GSTM3 was negatively associated to oxidative DNA damage (P < 0.05). Finally, the ability to predict spermiogram alterations was determined by ROC curve analysis using GSTM3 alone (AUC of 0.89; P < 0.05), and in combination with oxidative DNA damage (AUC of 0.91; P < 0.05).
Conclusions. Although the limited sample size of the present study warrants further studies and clinical trials with larger sample sets, our results set the ground for using GSTM3 as a novel biomarker and therapy target for male infertility and oxidative DNA damage in sperm.
A comprehensive expression profiles and prognostic values analysis of inosine 5'-monophosphate dehydrogenase type II (IMPDH2) in human tumors Running title: A pan-cancer analysis of IMPDH2
Authors: Huang Zigao , Cao Jiahua , Lao Jingmao , Deng Wei
Published: November 2022

Background
Inosine 5'-monophosphate dehydrogenase type II (IMPDH2) was thought to be involved in cancer initiation, progression, and treatment, but its biological role and underlying mechanism in pan-cancers are not fully studied. Our goal was to systematically investigate expression status and prognostic value through online data mining.
Methods
The expression and prognostic value of IMPDH2 in pan-cancer were analyzed using the HPA, GEPIA, TIMER, UALCAN, PrognoScan, Kaplan–Meier Plotter and DriverDBv3, and confirmed by immunohistochemical staining in gastric cancer. Function analysis was determined with GeneMANIA, STRING, and Metascape interactive tools, and alterations were examined by cBioPortal and UCSC Xena. Moreover, TISIDB and TIMER were applied to explore the relationships between IMPDH2 expression and immune infiltration, subtypes.
Results
Upregulation of IMPDH2 was observed in multiple human cancer tissues and relevant to a poor survival. Interactive analysis indicated that IMPDH2 was significantly associated with the metabolism of nucleotides, nucleobase metabolic process and regulation of canonical Wnt signaling pathway. Notably, a heatmap revealed positive correlations of IMPDH2 expression with macrophage M2, common lymphoid progenitor, myeloid derived suppressor cells, and negative correlations with macrophage M1, T cell follicular helper, B cell, T cell CD8+, dendritic cell. Additionally, there was a significant association between the IMPDH2 copy number, DNA methylation, exon expression levels and disease-free interval.
Conclusions
Together, IMPDH2 could act as a novel biomarker for the prognostic and immunological role in multiple human cancers.
The pre-surgical role of halo-traction in patients with cervical infection associated with refractory kyphosis. A retrospective study
Authors: Manini Daudi R. , Zhang Hong-Qi , Gao Qile , Liu Shao-Hua , YuXiang Wang , Tang Ming-Xing , An Deng , Guo Chao-Feng , YuXuan Du
Published: November 2022

To minimize surgical complications and staged procedures halo-traction is often used during deformity corrections. But the use of halo-traction in the treatment of rigid cervical kyphosis secondary to infections with abscesses has never been reported. This study investigated the role of halo-traction in the treatment of cervical infection patients associated with refractory kyphosis. We retrospectively reviewed 48 patients with cervical infection associated with refractory kyphosis who were treated in our spine department. Patients were divided into two groups, the traction group (A) and the non-traction group (B). Group A underwent preoperative halo-traction followed by surgery, while group B underwent surgery alone. Between the two groups, we analyzed the kyphosis deformity correction, level of fusions, disease clearing rate by erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), pain improvement by Oswestry disability index (ODI), and complications. Group A had a better correction of kyphosis deformity compared to group B (10.26±3.61)0 versus（12.24± 2.4)0 (P=0.04) ). No statistically significant differences between the two groups in terms of disease-clearing rate, pain improvement, level of fusion, and complications. Preoperative halo-traction followed by surgery is superior in kyphosis correction in the treatment of patients with cervical infections with refractory kyphosis.
A Novel Hybrid Arithmetic Optimization Algorithm and Salp Swarm Algorithm for Data Placement in Cloud Computing
Authors: awad ahmed , D.Abdellatif Ashraf , Alburaikan Alhanouf , Khalifa Hamiden , Elaziz Mohamed Abd , Abualigah Laith , M.AbdelMouty Ahmed
Published: November 2022

In recent years, the Internet of Things (IoT) has led to the spread of cloud computing devices in all commercial, industrial and agricultural sectors. The use of cloud computing environment services is increasing exponentially with all technology applications based on IoT. Fog computing has led to addressing issues in cloud computing environments. Fog computing reduces load balancing, processing, bandwidth, and storage as data file replication from the cloud to the network closest to sensors in different geographic locations. Traditional cloud computing leads to an increase in response time and processing time, and processing in the performance of data replication. We need replication strategies to meet users' requirements across different geographic locations while effectively harnessing fog computing capabilities to optimally select and place data replication of IoT services on cloud resources. In this strategy, the identification and mode of the data replication problem are designed as a multi-objective optimization problem that considers the heterogeneity of resources, least cost path, distance, and applications based on replication requirements. Firstly, a new hybrid metaheuristic method, using the Arithmetic Optimization Algorithm (AOA) and the salp swarm algorithm (SSA), is proposed to handle the problem of selection and placement data replication in fog computing. This strategy, called AOASSA, depends on using fog computing to optimally select and place data replication of IoT services on cloud resources. Secondly, the Floyd algorithm is used to strategy the least cost path, distance, and data transmission in different geographic locations. To validate the AOASSA strategy a set of experiments was carried out to validate the proposed strategy AOASSA. The performance of AOASSA is tested and compared with other swarm intelligence. Experiment results show the superiority of AOASSA over its competitors in terms of performance measures, such as least cost path, distance, and bandwidth.
Analysis and Pharmaceutical Applications of Serine protease from Arachis hypogaea
Authors: Shinde Shivam , Bhatia Manish S. , Halingale Omkar S.
Published: November 2022

Introduction:Serine protease is demonstrated to exist in both eukaryotes and prokaryotes. Serine proteases are present in the genomes of all cellular life forms, including many viruses, and are widely distributed in nature. Proteolytic enzymes that have attracted the most research attention are serine proteases.
Aim: This article highlights we have analysed the enzyme with various pH and temperature also developed the applications of serine protease enzyme.
Result:Serine protease enzyme is most stable at neutral ph i.e. pH &7.5 to 8 and at -4 ⁰C to 0 ⁰C. Critical analysis of the knowledge about serine protease from Arachis hypogaea source can help in the creation of pharmaceutical applications.
Discussion:The primary goals of the current effort are the creation of assays and the isolation of serine protease for pharmaceutical use. The enzyme serine protease is used to diagnose hemorrhagic or thrombotic disease co-agulation factors levels may also require. Targeted source was chosen, and the extraction procedure was constructed to control variables like pH and temperature for the right condition of the enzyme.
Conclusion:
Serine proteases were successfully isolated from Groundnut. Qualitative and quantitative characterization of the isolated serine proteases yielded consistent results.
The Representation of Greenery in the Boundaries Between the Open Landscape and Residential Areas in Suburbanized Rural Settlements. Development From the 19th to the 21st Century
Authors: Maňas Jan , Kabrhel Jan , Kyselovič Jakub
Published: November 2022

The rapid development of rural settlements into commuter towns in the hinterland of large cities, coupled with intensive agriculture and climate change, may negatively affect the prosperity of these areas. The presented analysis focused on randomly selected rapidly growing settlements in the Prague metropolitan area, examining their development with a focus on the peripheral parts of such settlements. Settlement margins constitute a specific part of rural settlements as it typically contributes to the rural character by providing the gradual gradient between the settlement and the open landscape.
The representation of water-absorbing and non-water absorbing areas and their suitability for the development of taller vegetation along the margins of three randomly selected suburbanized settlements in the hinterland of Prague are analysed from the perspective of the possibility to maintain the traditional character of the settlement margins. Historical maps, aerial photographs, spatial planning documentation and documents, and data on current land use and landscape cover were used for the analysis of the settlements’ development over the last approx. 200 years.
We have observed the trend of decreasing plot size and of reduction of the water-absorbing surfaces suitable for planting taller vegetation, especially in the 21st century residential developments, which disrupts the traditional rural character of these settlements. The utilization and stabilization of the current vegetation in the historical areas, together with the creation of the vegetation belts along the margins of the newly developed areas, could improve the environmental quality of these settlements, promote ecosystem services and increase biodiversity.
Application of radical mastectomy in ambulatory surgery centres under COVID-19
Authors: Zhou Jiao , Xiao Xiaoxiao , Yang Jiabin , Feng Yu , Qin Xiangquan , Li Juan , Yang Huanzuo , Qiu MengXue , Liu Yang , Huang Mingjun , Liang Peng , Du Zhenggui
Published: November 2022

Background Breast cancer has surpassed lung cancer as the most common cancer. Day surgery for breast cancer has been widely carried out worldwide, but the development of day surgery in China is relatively slow. To reduce the spread of coronavirus 2019 (COVID-19), major surgery centres have optimized the management procedures of day surgery to different degrees. However, relevant research on whether the rapid turnover of day surgery and the excessive human resources placed in the prevention and control of the COVID-19 epidemic will affect the quality of day surgery is lacking. Method The demographic data, clinical data and postoperative complications of breast cancer patients in the single medical group of West China Hospital of Sichuan University from March 2020 to June 2021 were retrospectively collected, and the complications after discharge and the safety of day surgery were analysed. Results The average age significantly differed between the ward surgery group (WS) and the day surgery group (DS) (P = 0.030). Regarding postoperative complications, no significant differences were detected in total surgical complications (P = 0.676) or anaesthesia complications (P = 0.126) between the two groups. In the logistic analysis, day surgery was not a risk factor for postoperative complications during the COVID-19 pandemic (P = 0.676, OR = 1.154, 95% CI: 0.590–2.257). An increase in age significantly increased the incidence of postoperative surgical complications (P = 0.024, OR = 1.051, 95% CI: 1.007–1.097). At the same time, lymph node dissection after sentinel lymph node biopsy also led to an increase in the incidence of postoperative surgical complications (P = 0.030, OR = 3.372, 95% CI: 1.125–10.106). In the survival curve, no significant difference in DFS was detected between the two groups (P = 0.353). Conclusion Radical mastectomy at day surgery centres is safe and reliable under strict COVID-19 management guidelines.
Multiple Adverse Events of anti-PD1/PDL1 Therapy: A Case- control Study using a Japanese Prescription-diagnostics Database
Authors: Hamano Hirofumi , Miyata Koji , Hara Tomoyo , Sakaguchi Satoshi , Oura Masahiro , Niimura Takahiro , Aizawa Fuka , Yagi Kenta , Okada Naoto , Miki Hirokazu , Goda Mitsuhiro , Ishizawa-Izawa Yuki , Zamami Yoshito , Yanagawa Hiroaki , Ishizawa Keisuke
Published: November 2022

Purpose
Accumulation of data on patient response to immune checkpoint inhibitors (ICIs) has enabled the analysis of adverse events (AEs) that have been difficult to analyze to date. The development of multiple AEs is often fatal; therefore, their characterization is important. Hence, we retrospectively investigated anti-programmed cell death protein 1 (PD1)/ programmed death-ligand 1 (PDL1) therapy by conducting a case-control study to determine the clinical characteristics of AEs associated with multiple ICIs.
Methods
We utilized the Japan Medical Data Center claims database, a prescription-diagnostics database, to obtain records of patients treated with PD1/PDL1 inhibitors, which were then analyzed. A total of 116,091 ICI-associated prescription-diagnostics data reports were retrieved. ICI-associated safety reports of individual cases till November 2020 were also analyzed. Furthermore, a narrowed search for immune-related AEs (irAEs) yielded 3,173 cases from the prescription-diagnostics data.
Results
There was a high incidence of concomitant AEs, and endocrine dysfunctions (hypophysitis, thyroiditis, and adrenal complications) had a high co-occurrence rate. Diabetes mellitus, hepatitis, and colitis overlapped in patients who developed thyroid dysfunction. In addition, there was a high incidence of myositis, myasthenia gravis, and myocarditis co-occurrence. Further analysis revealed a high mortality rate for interstitial pneumonia and diabetes mellitus (38%). In concomitant cases of myocarditis and other irAEs, severe cardiac diseases were developed.
Conclusion
Anti-PD1/PDL1 therapy may cause fatal clinical manifestations when accompanied by multiple irAEs. This study may provide adequate awareness among healthcare providers toward these characteristics.
Comprehensive study on clinical features, prognoses and histogenesis of combined hepatocellular-cholangiocarcinoma
Authors: Hu Junwen , Chen Jiahao , Yang Chenglei , Qi Yapeng , Zhang Jie , Zhong Jianhong , Xiang Bangde
Published: November 2022

Background:Combined hepatocellular-cholangiocarcinoma (CHC) is a rare subtype of primary liver cancer with histologic features of both hepatocellular carcinoma (HCC) andintrahepatic cholangiocarcinoma (ICC). The clinicopathological features，prognoses and histogenesis of CHC is unknown because of its low morbidity.
Methods: Twenty six patients with CHC, 901 with HCC, and 40 with ICC were included into analysis in this retrospective study. Their clinicopathological characteristics and prognosis were compared.
Results: CHC had clinicopathological features of both HCC and ICC. Patients with CHC had similar overall and disease-free survival with those with ICC, but all had significantly lower than those with HCC. Univariate analysis showed that AFP, CA19-9, CEA, tumor size, macrovascular invasion and lymph node metastasis were risk factors of both overall and disease-free survival. Multivariate analysis identified macrovascular invasion as independent prognostic factors for patients with CHC. Immunohistochemistry showed that CHC patients were with highly expression of CD90 and EpCAM.
Conclusions: Patients with CHC appear to have an intermediate clinical features compared with the HCC and ICC patients. The high expression of hepatic progenitor cell (HPC) markers in tumor tissues suggests that CHC may originate from HPCs.
AHP and GIS based land suitability analysis for built up in Bharatpur Metropolitan City, Nepal
Authors: Lamichhane Sadhuram
Published: November 2022

In Bharatpur Metropolitan City (BMC), rapid changes in socioeconomic conditions led to rapid urban growth, which in turn caused a number of environmental damages, including land fragmentation, unauthorized settlements, unchecked land use conversion, rising environmental pollution, and anthropogenic disasters. The rate of migration has been dramatically rising recently. These forecasts illustrated the gradual migration of people from rural to urban locations. Arable land fragmentation and erratic land use shifts are greatly impacted by rapid urbanization. They are significant concerns for property developers and urban planners. Urban planners and developers are unable to foresee city growth and modeling in the future using accurate management techniques due to unpredictable urban expansion. GIS based multi criteria decision making (MCDM) for land suitability analysis. Analytic hierarchy process (AHP) is a combination of qualitative and quantitative method used to calculate the weights of multi criterion by using pairwise matrices. By this method the biophysical and socioeconomic factors underlying the expansion has identified. Suitability maps had been prepared for several classes: highly suitable, moderate suitable, less suitable and restricted area and its percentage of area.