bluuebunny/crossref_metadata_2025 huggingface dataset:
Xanthones Isolated from Cratoxylum cochinchinensis Reduced Oxidative Stress in Periodontal Ligament Stem Cells
Authors: Ruangsawasdi Nisarat , Boonnak Nawong , Pruksaniyom Chareerut , Rodanant Pirasut
Published: September 2023

Xanthone compounds from Cratoxylum cochinchinensis (C. cochinchinensis) have demonstrated antioxidant effects and potency in treating many inflammatory diseases. However, the efficiency of the three xanthone extracts isolated from the young fruit of this plant, i.e., two geranyloxy xanthones (F6, F8) and one 1,3,7-hydroxy xanthone (F137), as antioxidants and therapeutics for periodontal disease has not been evaluated. The aim of this study was to investigate the antioxidant effects of three xanthones isolated from C. cochinchinensis on periodontal ligament stem cells (PDLSCs) and their osteogenic differentiation. The antioxidant activity of the aqueous extracts was determined using a DPPH assay, and their cytotoxicity was evaluated using an MTT assay. H2O2 was used to induce intracellular stress, and the scavenging effect of the isolated compounds against reactive oxygen species (ROS) was analyzed with a fluorescence assay. The expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was evaluated, and the effects of the three compounds on PDLSCs osteogenic differentiation were investigated. The isolated compounds reduced both extracellular and intracellular ROS in a dose-dependent manner and induced the expression of Nrf2 and HO-1 in PDLSCs. Under redox conditions, these compounds potentiated PDLSCs osteogenic differentiation. Our study demonstrated that the hydroxy xanthones from C. cochinchinensis had antioxidant effects on the Nrf2/HO-1 pathway and might be effective therapeutic substrates for damage prevention and the regeneration of damaged periodontal tissues in periodontitis patients.
Cholinesterase Inhibitory and Anti-Inflammatory Activity of the Naphtho- and Thienobenzo-Triazole Photoproducts: Experimental and Computational Study
Authors: Mlakić Milena , Faraho Ivan , Odak Ilijana , Kovačević Borislav , Raspudić Anamarija , Šagud Ivana , Bosnar Martina , Škorić Irena , Barić Danijela
Published: September 2023

New 1,2,3-triazolo(thieno)stilbenes were synthesized as mixtures of isomers and efficiently photochemically transformed to their corresponding substituted thienobenzo/naphtho-triazoles in high isolated yields. The resulting photoproducts were studied as acetyl- (AChE) and butyrylcholinesterase (BChE) inhibitors without or with interconnected inhibition potential of TNF-α cytokine production. The most promising anti-inflammatory activity was shown again by naphtho-triazoles, with a derivative featuring 4-pentenyl substituents exhibiting notable potential as a cholinesterase inhibitor. To identify interactions between ligands and the active site of cholinesterases, molecular docking was performed for the best potential inhibitors. Additionally, molecular dynamics simulations were employed to assess and validate the stability and flexibility of the protein–ligand complexes generated through docking.
Pharmacohistory of Cannabis Use—A New Possibility in Future Drug Development for Gastrointestinal Diseases
Authors: Thapa Dinesh , Warne Leon N. , Falasca Marco
Published: September 2023

Humans have employed cannabis for multiple uses including medicine, recreation, food, and fibre. The various components such as roots, flowers, seeds, and leaves have been utilized to alleviate pain, inflammation, anxiety, and gastrointestinal disorders like nausea, vomiting, diarrhoea, and inflammatory bowel diseases (IBDs). It has occupied a significant space in ethnomedicines across cultures and religions. Despite multi-dimensional uses, the global prohibition of cannabis by the USA through the introduction of the Marijuana Tax Act in 1937 led to prejudice about the perceived risks of cannabis, overshadowing its medicinal potential. Nevertheless, the discovery of tetrahydrocannabinol (THC), the primary psychoactive compound in cannabis, and the endocannabinoid system renewed scientific interest in understanding the role of cannabis in modulating different conditions, including gastrointestinal disorders. Preparations combining cannabidiol and THC have shown promise in mitigating gut symptoms through anti-inflammatory and motility-enhancing effects. This review revisits the ethnomedicinal use of cannabis in gastrointestinal diseases and emphasizes the need for further research to determine optimal dosages, formulations, and safety profiles of cannabis-based medicines. It also underscores the future potential of cannabinoid-based therapies by leveraging the role of the expanded endocannabinoid system, an endocannabinoidome, in the modulation of gastrointestinal ailments.
PSD95 as a New Potential Therapeutic Target of Osteoarthritis: A Study of the Identification of Hub Genes through Self-Contrast Model
Authors: Huang Ping , Lin Jieming , Shen Hongxing , Zhao Xiang
Published: September 2023

Osteoarthritis (OA) is a worldwide joint disease. However, the precise mechanism causing OA remains unclear. Our primary aim was to identify vital biomarkers associated with the mechano-inflammatory aspect of OA, providing potential diagnostic and therapeutic targets for OA. Thirty OA patients who underwent total knee arthroplasty were recruited, and cartilage samples were obtained from both the lateral tibial plateau (LTP) and medial tibial plateau (MTP). GO and KEGG enrichment analyses were performed, and the protein–protein interaction (PPI) assessment was conducted for hub genes. The effect of PSD95 inhibition on cartilage degeneration was also conducted and analyzed. A total of 1247 upregulated and 244 downregulated DEGs were identified. Significant differences were observed between MTP and LTP in mechanical stress-related genes and activated sensory neurons based on a self-contrast model of human knee OA. Cluster analysis identified DLG4 as the hub gene. Cyclic loading stress increased PSD95 (encoded by DLG4) expression in LTP cartilage, and PSD95 inhibitors could alleviate OA progression. This study suggests that inhibiting PSD95 could be a potential therapeutic strategy for preventing articular cartilage degradation.
Porous and Close Packed Supramolecular Assemblies from 2,4-Difluoronitrobenzene with Three Different Linkers and an n-Butylamine Cap
Authors: Plater M. John , Esslemont Abbie J. , Harrison William T. A.
Published: September 2023

A porous structure formed from sheets with cavities and two close packed structures were crystallised from building blocks prepared from 2,4-difluoronitrobenzene, a diamine linker and n-butylamine. The porous structure crystallised from a flexible building block prepared using 1,4-diaminobutane as linker. The close packed structures were prepared using either piperazine or 1,4-bis(aminomethyl)benzene as a linker and have less conformational freedom.
Chitosan Microparticles Enhance the Intestinal Release and Immune Response of an Immune Stimulant Peptide in Oncorhynchus mykiss
Authors: González-Chavarría Iván , Roa Francisco J. , Sandoval Felipe , Muñoz-Flores Carolina , Kappes Tomas , Acosta Jannel , Bertinat Romina , Altamirano Claudia , Valenzuela Ariel , Sánchez Oliberto , Fernández Katherina , Toledo Jorge R.
Published: September 2023

The aquaculture industry is constantly increasing its fish production to provide enough products to maintain fish consumption worldwide. However, the increased production generates susceptibility to infectious diseases that cause losses of millions of dollars to the industry. Conventional treatments are based on antibiotics and antivirals to reduce the incidence of pathogens, but they have disadvantages, such as antibiotic resistance generation, antibiotic residues in fish, and environmental damage. Instead, functional foods with active compounds, especially antimicrobial peptides that allow the generation of prophylaxis against infections, provide an interesting alternative, but protection against gastric degradation is challenging. In this study, we evaluated a new immunomodulatory recombinant peptide, CATH–FLA, which is encapsulated in chitosan microparticles to avoid gastric degradation. The microparticles were prepared using a spray drying method. The peptide release from the microparticles was evaluated at gastric and intestinal pH, both in vitro and in vivo. Finally, the biological activity of the formulation was evaluated by measuring the expression of il-1β, il-8, ifn-γ, Ifn-α, and mx1 in the head kidney and intestinal tissues of rainbow trout (Oncorhynchus mykiss). The results showed that the chitosan microparticles protect the CATH–FLA recombinant peptide from gastric degradation, allowing its release in the intestinal portion of rainbow trout. The microparticle-protected CATH–FLA recombinant peptide increased the expression of il-1β, il-8, ifn-γ, ifn-α, and mx1 in the head kidney and intestine and improved the antiprotease activity in rainbow trout. These results suggest that the chitosan microparticle/CATH–FLA recombinant peptide could be a potential prophylactic alternative to conventional antibiotics for the treatment of infectious diseases in aquaculture.
Physical Exercise as Disease-Modifying Alternative against Alzheimer’s Disease: A Gut–Muscle–Brain Partnership
Authors: Cutuli Debora , Decandia Davide , Giacovazzo Giacomo , Coccurello Roberto
Published: September 2023

Alzheimer’s disease (AD) is a common cause of dementia characterized by neurodegenerative dysregulations, cognitive impairments, and neuropsychiatric symptoms. Physical exercise (PE) has emerged as a powerful tool for reducing chronic inflammation, improving overall health, and preventing cognitive decline. The connection between the immune system, gut microbiota (GM), and neuroinflammation highlights the role of the gut–brain axis in maintaining brain health and preventing neurodegenerative diseases. Neglected so far, PE has beneficial effects on microbial composition and diversity, thus providing the potential to alleviate neurological symptoms. There is bidirectional communication between the gut and muscle, with GM diversity modulation and short-chain fatty acid (SCFA) production affecting muscle metabolism and preservation, and muscle activity/exercise in turn inducing significant changes in GM composition, functionality, diversity, and SCFA production. This gut–muscle and muscle–gut interplay can then modulate cognition. For instance, irisin, an exercise-induced myokine, promotes neuroplasticity and cognitive function through BDNF signaling. Irisin and muscle-generated BDNF may mediate the positive effects of physical activity against some aspects of AD pathophysiology through the interaction of exercise with the gut microbial ecosystem, neural plasticity, anti-inflammatory signaling pathways, and neurogenesis. Understanding gut–muscle–brain interconnections hold promise for developing strategies to promote brain health, fight age-associated cognitive decline, and improve muscle health and longevity.
Quantification of Oligonucleotides Using Tandem Mass Spectrometry with Isobaric Internal Standards
Authors: Gawlig Christopher , Hanci Güngör , Rühl Michael
Published: September 2023

In recent years, oligonucleotides have become more important in research, drug approvals and medical therapies. Due to this growing interest in pharmaceutical applications, it is essential to develop reliable analytical methods for this substance class. In this work, we present a quantification method using liquid chromatography coupled with tandem mass spectrometry by applying an isobaric oligonucleotide standard. In addition to a proof of principle, we perform a method qualification to assess its readiness for validation according to ICH Q2 guidelines. In addition to good linearity, sensitivity, accuracy and recovery, the method showed no significant matrix effects. Furthermore, we demonstrated the application of the method by applying the quantification in a biological matrix, as well as an exemplary degradation of an oligonucleotide in bovine plasma.
Application of Pluronics for Enhancing Aqueous Solubility of Lipophilic Microtubule Destabilizing Compounds on the Sea Urchin Embryo Model
Authors: Semenova Marina N. , Melik-Nubarov Nikolay S. , Semenov Victor V.
Published: September 2023

In screening, the dilution of DMSO stock solution of a lipophilic molecule with an assay medium often causes compound precipitation. To overcome the issue, the application of Pluronics as cosolvents was examined using a phenotypic sea urchin embryo assay that allows for the quick and facile evaluation of the antiproliferative effect together with systemic toxicity. Maximum tolerated concentration values for Pluronics L121, P123, and F127 were 1.4 μM, 8.6 μM, and 39.7 μM, respectively, and correlated directly with their hydrophilicity. Pluronics L121 and P123 suppressed cleavage and blastomeres retained the round shape, unlike hydrophilic Pluronic F127, which induced fertilization envelope creasing and embryo deformation that could be associated with the interaction of hydrophilic PEO units with mucopolysaccharides at the surface of sea urchin embryos. The toxicity of P123, but not of L121 and F127, was temperature-dependent and markedly increased at lower temperatures. CMC values obtained at different temperatures confirmed that the toxic effect of P123 was associated with both unimers and micelles, whereas F127 toxicity was related mainly to micelles. Evaluation using phenotypic sea urchin embryo assay revealed that potent microtubule destabilizers, namely albendazole, diarylisoxazole, and two chalcones, retained antimitotic activity after the dilution of their DMSO or 2-pyrrolidone stock solutions with 1.25% w/v Pluronic P123 or 5% w/v Pluronic F127. It was suggested that Pluronic P123 and Pluronic F127 could be used as cosolvents to improve the solubility of lipophilic molecules in aqueous medium.
Low-Temperature-Mediated Promoter Methylation Relates to the Expression of TaPOR2D, Affecting the Level of Chlorophyll Accumulation in Albino Wheat (Triticum aestivum L.)
Authors: Du Jingjing , Wang Junwei , Shan Sicong , Mi Tian , Song Yulong , Xia Yu , Ma Shoucai , Zhang Gaisheng , Ma Lingjian , Niu Na
Published: September 2023

Chlorophyll is an indispensable photoreceptor in plant photosynthesis. Its anabolic imbalance is detrimental to individual growth and development. As an essential epigenetic modification, DNA methylation can induce phenotypic variations, such as leaf color transformation, by regulating gene expression. Albino line XN1376B is a natural mutation of winter wheat cultivar XN1376; however, the regulatory mechanism of its albinism is still unclear. In this study, we found that low temperatures induced albinism in XN1376B. The number of chloroplasts decreased as the phenomenon of bleaching intensified and the fence tissue and sponge tissue slowly dissolved. We identified six distinct TaPOR (protochlorophyllide oxidoreductase) genes in the wheat genome, and TaPOR2D was deemed to be related to the phenomenon of albinism based on the expression in different color leaves (green leaves, white leaves and returned green leaves) and the analysis of promoters’ cis-acting elements. TaPOR2D was localized to chloroplasts. TaPOR2D overexpression (TaPOR2D-OE) enhanced the chlorophyll significantly in Arabidopsis, especially at two weeks; the amount of chlorophyll was 6.46 mg/L higher than in WT. The methylation rate of the TaPOR2D promoter in low-temperature albino leaves is as high as 93%, whereas there was no methylation in green leaves. Correspondingly, three DNA methyltransferase genes (TaMET1, TaDRM and TaCMT) were up-regulated in white leaves. Our study clarified that the expression of TaPOR2D is associated with its promoter methylation at a low temperature; it affects the level of chlorophyll accumulation, which probably causes the abnormal development of plant chloroplasts in albino wheat XN1376B. The results provide a theoretical basis for in-depth analysis of the regulation of development of plant chloroplasts and color variation in wheat XN1376B leaves.
Comparative Analysis of Deep Learning Architectures and Vision Transformers for Musical Key Estimation
Authors: Garg Manav , Gajjar Pranshav , Shah Pooja , Shukla Madhu , Acharya Biswaranjan , Gerogiannis Vassilis C. , Kanavos Andreas
Published: September 2023

The musical key serves as a crucial element in a piece, offering vital insights into the tonal center, harmonic structure, and chord progressions while enabling tasks such as transposition and arrangement. Moreover, accurate key estimation finds practical applications in music recommendation systems and automatic music transcription, making it relevant across academic and industrial domains. This paper presents a comprehensive comparison between standard deep learning architectures and emerging vision transformers, leveraging their success in various domains. We evaluate their performance on a specific subset of the GTZAN dataset, analyzing six different deep learning models. Our results demonstrate that DenseNet, a conventional deep learning architecture, achieves remarkable accuracy of 91.64%, outperforming vision transformers. However, we delve deeper into the analysis to shed light on the temporal characteristics of each deep learning model. Notably, the vision transformer and SWIN transformer exhibit a slight decrease in overall performance (1.82% and 2.29%, respectively), yet they demonstrate superior performance in temporal metrics compared to the DenseNet architecture. The significance of our findings lies in their contribution to the field of musical key estimation, where accurate and efficient algorithms play a pivotal role. By examining the strengths and weaknesses of deep learning architectures and vision transformers, we can gain valuable insights for practical implementations, particularly in music recommendation systems and automatic music transcription. Our research provides a foundation for future advancements and encourages further exploration in this area.
Luminescent Diimine-Pt(IV) Complexes with Axial Phenyl Selenide Ligands
Authors: Aseman Marzieh Dadkhah , Aghakhanpour Reza Babadi , Sharifioliaei Zohreh , Klein Axel , Nabavizadeh S. Masoud
Published: September 2023

Luminescent diimine-Pt(IV) complexes [Pt(N^N)(Me)2(PhSe)2], (N^N = 2,2′-bipyridine (bpy, 1b), 1,10-phenanthroline (phen, 2b), and 4,4′-dimethyl-2,2′-bipyridine (Me2bpy, 3b), PhSe− = phenyl selenide were prepared and identified using multinuclear (1H, 13C{1H} and 77Se{1H}) NMR spectroscopy. The PhSe− ligands were introduced through oxidative addition of diphenyl diselenide to the non-luminescent Pt(II) precursors [Pt(N^N)(Me)2], N^N = (bpy, 1a), (phen, 2a), (Me2bpy, 3a), to give the luminescent Pt(IV) complexes 1b–3b. The UV-vis absorption spectra of 1b–3b are characterised by intense bands in the range 240–330 nm. We assigned them to transitions of essentially π−π* character with small metal and PhSe− ligand contributions with the help of TD-DFT (time-dependent density functional theory) calculations. The weak long-wavelength bands in the range 350–475 nm are of mixed ligand-to-metal charge transfer (L’MCT) (n(Se)→d(Pt)/intra-ligand charge transfer (IL’CT) (n(Se)→π*(Ph) or π(Ph)→π*(Ph))/ligand-to-ligand’ charge transfer (LL’CT) (L = N^N, L’ = PhSe−, M = Pt and n = lone pair) character. The Pt(IV) complexes showed broad emission bands in the solid state at 298 and 77 K, peaking at 560–595 nm with a blue shift upon cooling. Structured emission bands were obtained in the range 450–600 nm, with the maxima depending on the N^N ligands and the solvent polarity (CH2Cl2 vs. dimethyl sulfoxide (DMSO) and aqueous tris(hydroxymethyl)aminomethane hydrochloride (tris-HCl) buffer). The emissions originate from essentially ligand-centred triplet states (3LC) with mixed IL’CT/L’MCT contributions as concluded from the DFT calculation. Such dominating PhSe contributions to the emissive states are unprecedented in the world of luminescent diimine-Pt(IV) complexes.
Correction: Topuz et al. DOME: Discrete Oriented Muon Emission in GEANT4 Simulations. Instruments 2022, 6, 42
Authors: Topuz Ahmet Ilker , Kiisk Madis , Giammanco Andrea
Published: September 2023

In the original publication [...]
Fault Location Method for Overhead Power Line Based on a Multi-Hypothetical Sequential Analysis Using the Armitage Algorithm
Authors: Kulikov Aleksandr , Ilyushin Pavel , Loskutov Anton , Filippov Sergey
Published: September 2023

The use of modern methods for determining the fault location (FL) on overhead power lines (OHPLs), which have high accuracy and speed, contributes to the reliable operation of power systems. Various physical principles are used in FL devices for OHPLs, as well as various algorithms for calculating the distance to the FL. Some algorithms for FL on OHPLs use emergency mode parameters (EMP); other algorithms use measurement results based on wave methods. Many random factors that determine the magnitude of the error in calculating the distance to the FL affect the operation of FL devices by EMP. Methods based on deterministic procedures used in well-known FL devices for OHPLs do not take into account the influence of random factors, which significantly increases the time to search for the fault. The authors have developed a method of FL on OHPLs based on a multi-hypothetical sequential analysis using the Armitage algorithm. The task of recognizing a faulted section of an OHPL is formulated as a statistical problem. To do this, the inspection area of the OHPL is divided into many sections, followed by the implementation of the procedure for FL. The developed method makes it possible to adapt the distortions of currents and voltages on the emergency mode oscillograms to the conditions for estimating their parameters. The results of the calculations proved that the implementation of the developed method has practically no effect on the speed of the FL algorithm for the OHPL by EMP. This ensures the uniqueness of determining the faulted section of the OHPL under the influence of random factors, which leads to a significant reduction in the inspection area of the OHPL. The application of the developed method in FL devices for OHPLs will ensure the required reliability of power supply to consumers and reduce losses from power outages by minimizing the time to search for a fault.
Automatic Variable Selection Algorithms in Prognostic Factor Research in Neck Pain
Authors: Liew Bernard X. W. , Kovacs Francisco M. , Rügamer David , Royuela Ana
Published: September 2023

This study aims to compare the variable selection strategies of different machine learning (ML) and statistical algorithms in the prognosis of neck pain (NP) recovery. A total of 3001 participants with NP were included. Three dichotomous outcomes of an improvement in NP, arm pain (AP), and disability at 3 months follow-up were used. Twenty-five variables (twenty-eight parameters) were included as predictors. There were more parameters than variables, as some categorical variables had >2 levels. Eight modelling techniques were compared: stepwise regression based on unadjusted p values (stepP), on adjusted p values (stepPAdj), on Akaike information criterion (stepAIC), best subset regression (BestSubset) least absolute shrinkage and selection operator [LASSO], Minimax concave penalty (MCP), model-based boosting (mboost), and multivariate adaptive regression splines (MuARS). The algorithm that selected the fewest predictors was stepPAdj (number of predictors, p = 4 to 8). MuARS was the algorithm with the second fewest predictors selected (p = 9 to 14). The predictor selected by all algorithms with the largest coefficient magnitude was “having undergone a neuroreflexotherapy intervention” for NP (β = from 1.987 to 2.296) and AP (β = from 2.639 to 3.554), and “Imaging findings: spinal stenosis” (β = from −1.331 to −1.763) for disability. Stepwise regression based on adjusted p-values resulted in the sparsest models, which enhanced clinical interpretability. MuARS appears to provide the optimal balance between model sparsity whilst retaining high predictive performance across outcomes. Different algorithms produced similar performances but resulted in a different number of variables selected. Rather than relying on any single algorithm, confidence in the variable selection may be increased by using multiple algorithms.
Effect of Water-Based vs. Land-Based Exercise Intervention (postCOVIDkids) on Exercise Capacity, Fatigue, and Quality of Life in Children with Post COVID-19 Condition: A Randomized Controlled Trial
Authors: Ogonowska-Slodownik Anna , Labecka Marta Kinga , Maciejewska-Skrendo Agnieszka , McNamara Renae J. , Kaczmarczyk Katarzyna , Starczewski Michał , Gajewski Jan , Morgulec-Adamowicz Natalia
Published: September 2023

Evidence suggests that COVID-19 can cause lasting health consequences called post-COVID-19 condition. We conducted a three-group, randomized controlled trial for children aged 10–12 years with post COVID-19 condition. Participants were randomized to AQUA, LAND, or CONTROL groups. The AQUA and LAND training sessions were conducted twice a week for eight weeks. The primary outcomes were exercise capacity, measured using the modified Balke treadmill protocol, and fatigue, measured using the Cumulative Fatigue Symptoms Questionnaire (CFSQ). The secondary outcome was health-related quality of life (HRQoL), measured with the Pediatric Quality of Life Inventory (PedsQL) for children and parents. A total of 74 of the 86 children completed the intervention and attended the post-intervention assessment. The absolute maximal oxygen uptake (VO2max) values increased after both AQUA (p = 0.001) and LAND (p = 0.004) interventions. No significant differences were found in the degree of total fatigue and individual fatigue symptoms. A significant improvement was found in the PedsQL reported by the parents in the LAND group. In conclusion, the applied eight-week water-based and land-based exercise training programs improved exercise capacity in children aged 10–12 years old with post COVID-19 condition. The parents of the children in the training groups also noted an improvement in HRQoL.
Serum Fibrinogen and Renal Dysfunction as Important Predictors of Left Atrial Thrombosis in Patients with Atrial Fibrillation
Authors: Golubić Karlo , Angebrandt Belošević Petra , Slišković Ana Marija , Grubić Zorana , Štingl Janković Katarina , Radeljić Vjekoslav , Delić Brkljačić Diana
Published: September 2023

Background: As has been shown previously, patients with atrial fibrillation (AF) who have left atrial thrombus (LAT) also have elevated plasma concentrations of fibrinogen. In this study, we tried to determine if this is the consequence of a genetic trait and whether elevated concentrations of fibrinogen could be used to predict LAT in patients with AF. Methods: We recruited 181 consecutive patients scheduled for pulmonary vein isolation (PVI) or direct current cardioversion. The primary endpoint was the presence of LAT on transesophageal echocardiography (TOE). We recorded routine clinical and biochemical data as well as the polymorphism type of the fibrinogen gene for the β chain. To control potentially interfering variables, we performed propensity score matching (PSM). Multivariable and univariable logistic regression models (LRM) were computed using the CHA2DS2-Vasc score, the fibrinogen concentration and creatinine clearance as estimated by the Cockcroft–Gault equation. Results: 60 of 181 patients had LAT as detected by TOE. As expected, patients with LAT had significantly higher concentrations of fibrinogen (3.9 vs. 3.6 g/L); p = 0.01 in the unadjusted analysis. After performing PSM, there were no statistically significant differences between the groups, except for creatinine clearance (79.9 vs. 96.8 mL/min); p = 0.01. There were also no differences regarding the −455 G/A βfibrinogen polymorphism distribution between the two groups. After constructing the LRM, we found no performance enhancement for the CHA2DS2-Vasc score by adding the fibrinogen concentration or creatinine clearance alone, but when all three variables were put together, there was a significant improvement in LAT prediction (AUC 0.64 vs. 0.72), p = 0.026. Conclusion: Our study found no evidence of elevated levels of circulating fibrinogen in patients with LAT or a connection between those levels and the A/A and A positive polymorphism. When used together with renal function markers such as creatinine clearance, plasma fibrinogen concentrations can provide additional power to the CHA2DS2-Vasc score for predicting LAT.
The Impact of Sex on Cardiogenic Shock Outcomes Following ST Elevation Myocardial Infarction
Authors: Arnold Joshua H. , Perl Leor , Assali Abid , Codner Pablo , Greenberg Gabriel , Samara Abid , Porter Avital , Orvin Katia , Kornowski Ran , Vaknin Assa Hana
Published: September 2023

Background: Cardiogenic shock (CS) remains the leading cause of ST elevation myocardial infarction (STEMI)-related mortality. Contemporary studies have shown no sex-related differences in mortality. Methods: STEMI-CS patients undergoing primary percutaneous coronary intervention (PPCI) were included based on a dedicated prospective STEMI database. We compared sex-specific differences in CS characteristics at baseline, during hospitalization, and in subsequent clinical outcomes. Endpoints included all-cause mortality and major adverse cardiac events (MACE). Results: Of 3202 consecutive STEMI patients, 210 (6.5%) had CS, of which 63 (30.0%) were women. Women were older than men (73.2 vs. 65.5% y, p < 0.01), and more had hypertension (68.3 vs. 52.8%, p = 0.019) and diabetes (38.7 vs. 24.8%, p = 0.047). Fewer were smokers (13.3 vs. 41.2%, p < 0.01), had previous PCI (9.1 vs. 22.3% p = 0.016), or required IABP (35.3 vs. 51.1% p = 0.027). Women had higher rates of mortality (53.2 vs. 35.3% in-hospital, p = 0.01; 61.3 vs. 41.9% at 1 month, p = 0.01; and 73.8 vs. 52.6% at 3 years, p = 0.05) and MACE (60.6 vs. 41.6% in-hospital, p = 0.032; 66.1 vs. 45.6% at 1 month, p = 0.007; and 62.9 vs. 80.3% at 3 years, p = 0.015). After multivariate adjustment, female sex remained an independent factor for death (HR-2.42 [95% CI 1.014–5.033], p = 0.042) and MACE (HR-1.91 [95% CI 1.217–3.031], p = 0.01). Conclusions: CS complicating STEMI is associated with greater short- and long-term mortality and MACE in women. Sex-focused measures to improve diagnosis and treatment are mandatory for CS patients.
Hospital Admission Factors Independently Affecting the Risk of Mortality of COVID-19 Patients
Authors: Paciorek Marcin , Bieńkowski Carlo , Kowalska Justyna Dominika , Skrzat-Klapaczyńska Agata , Bednarska Agnieszka , Krogulec Dominika , Cholewińska Grażyna , Kowalski Jacek , Podlasin Regina , Ropelewska-Łącka Katarzyna , Wasilewski Piotr , Boros Piotr W. , Martusiewicz-Boros Magdalena M. , Pulik Piotr , Pihowicz Andrzej , Horban Andrzej
Published: September 2023

Introduction: COVID-19 is a disease characterized by high in-hospital mortality, which seems to be dependent on many predisposing factors. Objectives: The aim of this study was to analyze the clinical symptoms, abnormalities in the results of laboratory tests, and coexisting chronic diseases that independently affected the risk of in-hospital mortality in patients with COVID-19. Patients and Methods: We analyzed the records of patients with COVID-19 who were hospitalized from 6 March 2020 to 30 November 2021. Results: Out of the entire group of 2138 patients who were analyzed, 12.82% died during hospitalization. In-hospital mortality was independently associated with older age (OR 1.53, 95% CI 1.20–1.97); lower arterial blood oxygen saturation (OR 0.95, 95% CI 0.92–0.99); the presence of a neoplasm (OR 4.45, 95% CI 2.01–9.62), a stomach ulcer (OR 3.35, 95% CI 0.94–11.31), and dementia (OR 3.40, 95% CI 1.36–8.26); a higher score on the SOFA scale (OR 1.73, 95% CI 1.52–1.99); higher lactate dehydrogenase (LDH) (OR 1.08, 95% CI 1.05–1.12); higher N-terminal pro-brain natriuretic peptide (NT pro BNP) (OR 1.06, 95% CI 1.01–1.11); and lower total bilirubin in blood concentration (OR 0.94, 95% CI 0.90–0.99). Conclusions: We found that low oxygen saturation, old age, and the coexistence of cancer, gastric ulcers, and dementia syndrome were variables that independently increased mortality during hospitalization due to COVID-19. Moreover, we found that decreased platelet count and bilirubin concentration and increased levels of LDH and NT-proBNP were laboratory test results that independently indicated a higher risk of mortality. We also confirmed the usefulness of the SOFA scale in predicting treatment results. The ability to identify mortality risk factors on admission to hospital will facilitate both adjusting the intensity of treatment and the monitoring of patients infected with SARS-CoV-2.
Multiple Roles of the Low-Affinity Calcium Uptake System in Drechslerella dactyloides, a Nematode-Trapping Fungus That Forms Constricting Rings
Authors: Zhao Xiaozhou , Fan Yani , Zhang Liao , Zhang Weiwei , Xiang Meichun , Kang Seogchan , Wang Shunxian , Liu Xingzhong
Published: September 2023

(1) Background: the low-affinity calcium uptake system (LACS) has been shown to play a crucial role in the conidiation and formation of adhesive nets and knobs by nematode-trapping fungi (NTF), but its involvement in the formation of constricting rings (CRs), mechanical traps to capture free-living nematodes, remains unexplored. (2) Methods: we investigated the function of two LACS genes (DdaFIG_1 and DdaFIG_2) in Drechslerella dactyloides, an NTF that forms CRs. We generated single (DdaFIG_1Ri and DdaFIG_2Ri) and double (DdaFIG_1,2Ri) knockdown mutants via the use of RNA interference (RNAi). (3) Results: suppression of these genes significantly affected conidiation, trap formation, vegetative growth, and response to diverse abiotic stresses. The number of CRs formed by DdaFIG_1Ri, DdaFIG_2Ri, and DdaFIG_1,2Ri decreased to 58.5%, 59.1%, and 38.9% of the wild-type (WT) level, respectively. The ring cell inflation rate also decreased to 73.6%, 60.6%, and 48.8% of the WT level, respectively. (4) Conclusions: the LACS plays multiple critical roles in diverse NTF.
How University Students Evaluate the Role of Social Media in Political Polarization: Perspectives of a Sample of Turkish Undergraduate and Graduate Students
Authors: Wazzan Ahmad , Aldamen Yasmin
Published: September 2023

This study aimed to find out if there is a relationship between social media and political polarization in Türkiye from the perspective of Turkish students. To reach this aim, the needed data were collected through qualitative and quantitative approaches. A total of 303 valid questionnaires were analyzed. The sample consisted of university Turkish students across undergraduate, masters, and PhD levels in Türkiye aged between 18 and 50+. As well, an online focus group discussion with six Turkish students from different universities and education levels was conducted to gain a more in-depth understanding of the study’s problem. The results of the study showed that the perspectives of the Turkish students were that social media had a weak-to-moderate effect on political polarization in Türkiye. Furthermore, the results indicated that the studied sample of the Turkish students does not rely on social media platforms to obtain political news, and most of them do not follow political leaders on social media. Moreover, communication platforms did not encourage many Turkish students to express themselves, which is an indication that social media algorithms have contributed to a medium degree in creating filter bubbles through the content they suggest to users. Results have also shown that Turkish students are afraid that their posts and comments are being censored.
The Strange Case of Orotic Acid: The Different Expression of Pyrimidines Biosynthesis in Healthy Males and Females
Authors: Chiara Francesco , Allegra Sarah , Mula Jacopo , Puccinelli Maria Paola , Abbadessa Giuliana , Mengozzi Giulio , De Francia Silvia
Published: September 2023

Orotic acid (OA) is an intermediate metabolite of pyrimidine nucleotide biosynthesis and represents a minor diet constituent. The measurement of urinary orotic acid is useful in confirming the diagnosis of hereditary metabolic diseases. Moreover, it could be of interest to know how the physiological concentration of this metabolite changes in relation to different conditions of clinical normality. The purpose of this study was to determine the orotic acid concentration in the urine of healthy patients, to observe normal oroticuria and to evaluate if the expression of pyrimidine intermediate biosynthesis differs between healthy males and females. The orotic acid concentration in urine was performed via the ICH M10-validated analytical method. Unexpectedly, females showed a greater oroticuria than males in pediatric age (0–10); conversely, we did not find significant differences until 70 years of age. The LC-MS/MS method was suitable for use in the differential diagnosis of hereditary metabolic disease and metabolic monitoring of anticancer drug-induced toxicity. The analytical protocol was found to be rapid and ideal, and was used in the routine analysis of a clinical chemistry laboratory. The biochemical aspects related to the expression of pyrimidine biosynthesis should be further investigated in light of the obtained results.
Differentiation of Small Clear Renal Cell Carcinoma and Oncocytoma through Magnetic Resonance Imaging-Based Radiomics Analysis: Toward the End of Percutaneous Biopsy
Authors: Toffoli Thibault , Saut Olivier , Etchegaray Christele , Jambon Eva , Le Bras Yann , Grenier Nicolas , Marcelin Clément
Published: September 2023

Purpose: The aim of this study was to ascertain whether radiomics data can assist in differentiating small (<4 cm) clear cell renal cell carcinomas (ccRCCs) from small oncocytomas using T2-weighted magnetic resonance imaging (MRI). Material and Methods: This retrospective study incorporated 48 tumors, 28 of which were ccRCCs and 20 were oncocytomas. All tumors were less than 4 cm in size and had undergone pre-biopsy or pre-surgery MRI. Following image pre-processing, 102 radiomics features were evaluated. A univariate analysis was performed using the Wilcoxon rank-sum test with Bonferroni correction. We compared multiple radiomics pipelines of normalization, feature selection, and machine learning (ML) algorithms, including random forest (RF), logistic regression (LR), AdaBoost, K-nearest neighbor, and support vector machine, using a supervised ML approach. Results: No statistically significant features were identified via the univariate analysis with Bonferroni correction. The most effective algorithm was identified using a pipeline incorporating standard normalization, RF-based feature selection, and LR, which achieved an area under the curve (AUC) of 83%, accuracy of 73%, sensitivity of 79%, and specificity of 65%. Subsequently, the most significant features were identified from this algorithm, and two groups of uncorrelated features were established based on Pearson correlation scores. Using these features, an algorithm was established after a pipeline of standard normalization and LR, achieving an AUC of 90%, an accuracy of 77%, sensitivity of 83%, and specificity of 69% for distinguishing ccRCCs from oncocytomas. Conclusions: Radiomics analysis based on T2-weighted MRI can aid in distinguishing small ccRCCs from small oncocytomas. However, it is not superior to standard multiparameter renal MRI and does not yet allow us to dispense with percutaneous biopsy.
The Impact of Trophoblast Cell-Surface Antigen 2 Expression on the Survival of Patients with Gastrointestinal Tumors: A Systematic Review
Authors: Liatsou Efstathia , Schizas Dimitrios , Frountzas Maximos
Published: September 2023

Background: Trophoblast cell-surface antigen 2 (TROP-2) is a transmembrane glycoprotein expressed in epithelial cells that has been associated with malignant progression in most carcinomas. Accordingly, the genetic complexity of gastrointestinal tumors necessitates the investigation of new biomarkers with potential prognostic value. The aim of this systematic review is to assess the effect of TROP-2 on the overall survival of patients who underwent surgery for gastrointestinal malignancy. Methods: The present systematic review was designed using the PRISMA and AMSTAR guidelines. We searched the Pubmed, EMBASE and CENTRAL databases from their inception to September 2023. Results: Ten studies that enrolled 2293 patients were included for qualitative analysis. Six studies evaluated patients with colorectal cancer, two studies included patients with gastric carcinoma, patients with pancreatic cancer were included in one study and one study included hepatobiliary cancer patients. TROP-2 was positive in 1005/2293 samples of the immunohistochemically evaluated biopsies and was associated with poor overall survival in all studies. High intensity was also associated with more aggressive histopathological characteristics, such us deep tissue invasion, lymph node metastasis and cell atypia. The prognostic value of TROP-2 was shown to be enhanced in patients with advanced disease and poor histological differentiation. Conclusion: TROP-2 was expressed at high levels in gastrointestinal tumors, which was associated with both tumor development and pathological aggressiveness. Therefore, TROP-2 could be used as a biomarker to determine clinical prognosis and as a potential therapeutic target in malignancies of the gastrointestinal tract, but further studies need to validate it.
New Technology Deployment and Corporate Responsibilities in the Metaverse
Authors: Wynn Martin , Jones Peter
Published: September 2023

The term “metaverse” came to the fore in 2021 when Facebook rebranded its corporate identity to Meta and signalled its intention to invest at least USD 10 billion in developing the concepts and related products that year. However, there is still little consensus in defining what constitutes the metaverse, although there is a widespread, though not universal, agreement that it will bring a wide range of benefits across society. More specifically, the advent and continuing evolution of the metaverse has strategic and operational implications for, and impacts on, industry and business at large. Adopting an inductive, interpretivist approach, this exploratory research article presents case examples of the guidance on the responsible development of the metaverse provided by two IT business services companies. This article identifies the major risks and responsibilities associated with the metaverse and assesses how companies might address these responsibilities. Very little research has been published in this area, and this article attempts to make a small contribution to filling this gap in the literature. This article finds that these responsibilities are largely in line with those currently associated with corporate digital responsibility, and concludes that the strategic impact and extent of regulatory change will depend on the nature of the metaverse that materialises in the forthcoming decade.
Land Use/Land Cover Changes in Baicheng District, China during the Period 1954–2020 and Their Driving Forces
Authors: Peng Bin , Yang Jiuchun , Li Yixue , Zhang Shuwen
Published: September 2023

Temporal and spatial variations in land use/land cover (LULC) and their driving factors are direct reflections of regional natural and anthropogenic impacts. To explore the pathways for green upgrading development in ecologically fragile areas, this study focused on Baicheng, located in the northern agropastoral transition zone, China. Based on the topographic map of 1954 and Landsat remote sensing images taken from 1976 to 2020, the spatial distribution of LULC data for the study area in 1954, 1976, 1988, 2000, 2010, and 2020 was obtained. The temporal and spatial characteristics of LULC changes and their driving factors under the combined influence of human activities and climate were analyzed using dynamic degree, flowchart, spatial analysis, and principal component analysis. The results indicate that (1) the dominant LULC type in Baicheng is cropland. By 2020, dry land accounted for over 41% of the total area, while the area of saline–alkaline land increased the most, and grassland decreased most drastically. (2) The dynamic degree of different LULC types ranked from highest to lowest as follows: paddy field > unused land and other types > woodland > saline–alkaline land. (3) LULC Changes in Baicheng were mainly influenced by human activities and economic development, especially regional gross domestic product and the sown area of crops. These research findings can provide a scientific basis for formulating sustainable development and protection strategies to ensure regional green upgrading development.
Do Green Production Technologies Improve Household Income? Evidence from Rice Farmers in China
Authors: Kuang Foyuan , Li Jiatong , Jin Jianjun , Qiu Xin
Published: September 2023

Revealing the behavioral decision-making mechanism and behavioral effects of farmers adopting green production technologies will help motivate farmers to actively adopt green production technologies, thereby promoting the development of green agriculture. A stratified random sampling technique was used to select 607 Chinese rice farmers, while the endogenous switching regression model was used to analyze the influencing factors and behavioral effects of farmers adopting green production technologies. The results show that the adoption of green production technologies by farmers can significantly increase household income. Among the green production technologies, soil testing and formula fertilization technology has the greatest impact on farmers’ income, followed by straw returning technology and planting green manure. The main influencing factors of farmers adopting green production technologies are education level, cultivated land area, cultivated land fragmentation, soil fertility, and the distance between home and agricultural technology stations. The results of this study provide an empirical basis for relevant government departments to carry out agricultural technology extension work and formulate relevant policies.
Study of the Morphological Characteristics of Cultivated Land in Semiarid Sandy Areas
Authors: Zhao Xiangyu , Yan Wenzhi , Wang Kaige , Xu Yan , Zheng Huihui , Sang Zhiting
Published: September 2023

The unique natural geographical conditions and land use patterns in semiarid sandy areas have resulted in a distinct cultivated landscape. Identifying and classifying the morphological characteristics of cultivated land are key to improving land utilization efficiency. This study focuses on the semiarid sandy areas of China. We obtained information on cropland morphology by judging high-precision remote sensing images and landscape pattern analysis, and then determined the spatial clustering characteristics of different plot morphologies through local spatial autocorrelation analysis. The results are as follows: cultivated land can be classified into five main types: simple large plots, complex large plots, simple small plots, complex small plots, and scattered plots, and there are significant differences in morphology between eastern and western plots. In addition, different morphology types of plots formed a variety of aggregation patterns; clustered plots are surrounded by scattered plots, showing a staggered distribution pattern. Farmers and land managers can make more informed decisions regarding irrigation, fertilization, and crop selection, and this knowledge can provide a basis for further optimizing the layout of cultivated land, identifying fragmented cultivated land, and scientifically recovering farmland to ensure more scientific and refined classification management and zoning protection of cultivated land.