bluuebunny/crossref_metadata_2025 huggingface dataset:
Provenance of the He 8 Member of the Upper Paleozoic Shihezi Formation, Ordos Basin, China: Insights from Heavy Minerals, Paleocurrents, Detrital Zircon Chronology, and Hf Isotopes
Authors: Pan Wenqi , Jiang Ziwen , Fan Liyong , Zhang Zhengtao , Li Zhichao , Ma Shangwei , Wang Zhendong , Li Xiangjun , Zhao Weiran
Published: October 2024

The Ordos Basin is located in the western part of the North China Craton. The Upper Paleozoic Shihezi Formation, particularly the He 8 Member, is one of the main gas-bearing strata. However, the source areas for the north and south sections have not been clearly distinguished, which has constrained oil and gas exploration to some extent. Therefore, understanding the source rock evolution of He 8 Member in both the south and north basins will provide a favorable theoretical basis for oil and gas exploration. The provenance of the He 8 Member of the Shihezi Formation in the Ordos Basin has not been well defined until now. Seven wellbore sandstone samples and three field outcrop sandstone samples from the He 8 Member in the Ordos Basin were analyzed. Based on zircon U–Pb dating and Lu–Hf isotope analyses, zircon assemblages of 520–386 Ma and 350–268 Ma in the southern Ordos Basin might have originated from the North Qinling Orogenic Belt (NQinOB) and the North Qilian Orogenic Belt (NQiOB); the 350–268 Ma age group of zircons from the NQinOB, and a large number of ~320–260 Ma detrital zircons supplied to the southern Ordos Basin by the NQinOB suggest that NQinOB magmatic and/or metamorphic events may have occurred in the NQinOB during the ~320–260 Ma period. From ~320–260 Ma, the NQinOB might have experienced significant tectonic activity that has not been fully revealed thus far. The zircons from 2600–2300 Ma, 2000–1600 Ma, and 450–300 Ma in the northern Ordos Basin might have been derived from the Trans-North China Orogenic Belt (TNCO), the Khondalite Belt, the Yinshan Belt, and the Alxa Belt. The paleocurrent and heavy mineral analyses determined that there are certain differences between the northern Ordos Basin and southern Ordos Basin, with unstable minerals such as barite and pyrite, as well as moderately stable minerals such as garnet, showing an increasing trend from south to north. There are also differences in the dominant paleocurrent directions between the south and north parts of the basin, and the Hf isotope data in the Ordos Basin show two-stage Hf model ages (TDM2) ranging from 918 Ma to 3574 Ma. As a result, the He 8 Member deposits in the southern Ordos Basin and northern Ordos Basin had different sources. The southern Ordos Basin might have derived from the NQinOB, the NQiOB, and the TNCO, and the northern Ordos Basin might have derived from the TNCO, the Khondalite Belt, the Yinshan Belt, and the Alxa Belt.
The Impact of Biowaste Composition and Activated Carbon Structure on the Electrochemical Performance of Supercapacitors
Authors: Abdisattar Alisher , Yerdauletov Meir , Yeleuov Mukhtar , Napolskiy Filipp , Merkulov Aleksey , Rudnykh Anna , Nazarov Kuanysh , Kenessarin Murat , Zhomartova Ayazhan , Krivchenko Victor
Published: October 2024

The increasing demand for sustainable and efficient energy storage materials has led to significant research into utilizing waste biomass for producing activated carbons. This study investigates the impact of the structural properties of activated carbons derived from various lignocellulosic biomasses—barley straw, wheat straw, and wheat bran—on the electrochemical performance of supercapacitors. The Fourier Transform Infrared (FTIR) spectroscopy analysis reveals the presence of key functional groups and their transformations during carbonization and activation processes. The Raman spectra provide detailed insights into the structural features and defects in the carbon materials. The electrochemical tests indicate that the activated carbon’s specific capacitance and energy density are influenced by the biomass source. It is shown that the wheat-bran-based electrodes exhibit the highest performance. This research demonstrates the potential of waste-biomass-derived activated carbons as high-performance materials for energy storage applications, contributing to sustainable and efficient supercapacitor development.
Evaluation of the Potential of High-Performance Liquid Chromatography–Inductively Coupled Plasma–Mass Spectrometry for the Determination of Chemical Warfare Agents and Their Toxic Degradation Products
Authors: Kuligowska Monika , Neffe Slawomir
Published: October 2024

The determination of chemical warfare agents (CWAs) and their toxic degradation products (DPs) has become increasingly important for public and military safety in recent years. We focused on assessing the possibility of the HPLC-ICP-MS analytical technique to verify the provisions of the Chemical Weapons Convention. This technique enables the identification and determination of minimal concentrations (ppt range) of elements in various matrices. This fact is important for the determination of CWAs and other highly harmful compounds, even small amounts of which can have serious consequences for living organisms. We have critically analysed the results of scientific research on the identification and quantitative determination of extremely toxic organophosphorus, organosulfur and organoarsenic CWAs, their derivatives and their degradation products using high-performance liquid chromatography (HPLC) coupled with inductively coupled plasma–mass spectrometry (ICP-MS).
Keep Your TEMPO Up: Nitroxide Radicals as Sensors of Intermolecular Interactions
Authors: Shenderovich Ilya G.
Published: October 2024

This study examines experimental data on the influence of the surrounding medium and non-covalent interactions on the isotropic hyperfine coupling constant, Aiso(14N), of the stable nitroxide radical 2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) in solution. The data were used to identify a density functional theory functional/basis set combination that accurately reproduces the experimental Aiso(14N) values. The variations in Aiso(14N) due to external factors are two orders of magnitude greater than the accuracy of its experimental measurements, making Aiso(14N) a highly sensitive experimental probe for quantifying these effects. Additionally, it was found that the proton-accepting ability of the N-O• moiety in TEMPO resembles that of the P=O moiety, enabling the simultaneous formation of two equally strong hydrogen bonds.
A Simple HPLC-DAD Method for the Therapeutic Monitoring of Clozapine and Related Metabolites in Human Plasma and Urine Samples
Authors: Comănescu Mircea-Alexandru , Preda Dana-Maria , Miron Dalia-Simona , Rădulescu Flavian-Ștefan , Voicu Victor , Medvedovici Andrei-Valentin
Published: October 2024

Clozapine and its metabolites require close therapeutic monitoring (TDM) in patients due to poor correlation between the administrated doses and resulting plasma concentrations, the narrow therapeutic interval, high inter-individual variability, and the risk of serious side effects once toxic levels are exceeded. The aim of the study was to develop a simple (relatively cheap) LC-UV method for the quantification of clozapine and its metabolites in plasma and urine samples. For sample preparation, liquid-liquid extraction (LLE) in n-octanol was more efficient and less limiting in injection volumes compared to the in-situ formation of SUPRAS. When analyzing urine, an alkalinization step before extraction was required. The proposed method produced linear concentration responses with/without internal standard (IS) for the target analytes, with LLOQs within the targeted range of 50 ppb and %RSD within the acceptable 15% range. Furthermore, sample stability studies proved that pre-extracted samples were stable for the short term at room temperature and long-term when frozen.
Conjugated Diphenylaniline-Azulene Co-Oligomers with Intense Absorption and Emission in the Visible Region
Authors: Merkhatuly Nurlan , Iskanderov Amantay , Abeuova Saltanat , Iskanderov Ablaykhan , Zhokizhanova Saltanat
Published: October 2024

New conjugated 2,6-diphenylaniline-azulene co-oligomers of linear and branched structure were synthesized by the interaction of borylazulenes with diphenylaniline bromides under Suzuki–Miyaura cross-coupling conditions. The obtained diphenylaniline-azulene co-oligomers intensively absorb and emit visible light (410–700 nm region); in particular, they exhibit strong emissions in the green, as well as orange, range, with maxima of 510/590 nm. It is shown that such properties appear as a result of the positive resonance exposure to aniline fragments significantly rearranging the electronic structure of azulene, in particular, the levels and energy gaps of frontal HOMO–LUMO orbitals.
Shape Memory Polymer Foam Based on Nanofibrillar Composites of Polylactide/Polyamide
Authors: Elumalai Dhanumalayan , Hosseinnezhad Ramin , Bondarenko Vladislav , Morawiec Jerzy , Vozniak Iurii , Galeski Andrzej
Published: October 2024

This paper presents the novel development of a shape memory polymer foam based on polymer–polymer nanocomposites. Herein, polylactide (PLA)/biosourced polyamide (PA) foams are fabricated by in situ fibrillation of polymer blends and a subsequent supercritical CO2 foaming technique. In this system, PLA serves as a shape memory polymer to endow this foam with a shape memory effect (SME), and in situ generated PA nanofibers are employed to reinforce the PLA cell walls and provide an additional permanent phase. A concentration of PA, 5 wt.%, was chosen to form an entangled nanofibrillar network. Foams of PLA/PA nanoblends with the same content of constituents were fabricated to reveal the effect of minor phase morphology on the cell structure and shape memory behavior of polymer foams. Profiting from the reinforcing effect of PA nanofibers, the PLA/PA nanocomposite foam exhibits smaller foam cells, a narrower cell size distribution and a comparable cell concentration than the PLA/PA nanoblend foam. In addition, PA nanofibers, unlike PA nanodroplets, favor the shape fixation ratio and recovery ratio and shorten the shape recovery time.
New Two-Dimensional Materials Obtained by Functionalization of Boron Graphdiyne Layers with Nickel
Authors: Germán Estefanía , López María J. , Alonso Julio A.
Published: October 2024

The decoration of hexagonal boron graphdiyne (BGDY) layers with Ni atoms has been investigated by density functional calculations. For one, two, and three Ni atoms per hexagon, the BGDY structure is approximately maintained. Decoration with six Ni atoms per hexagon leads to the formation of a novel, very stable two-dimensional material in which the hexagonal structure of BGDY is substantially distorted. The Ni-doped materials have a semiconductor character, and the electronic band gap width can be tailored by varying the amount of adsorbed Ni. BGDY-2Ni, BGDY-3Ni, and BGDY-6Ni have electronic band gaps promising for infrared detectors. This work shows that computer simulation helps to discover new materials by the functionalization of layered carbon materials with metal atoms.
Ketogenic Diet Improves Sleep Quality and Daytime Sleepiness in Chronic Migraine: A Pilot Study
Authors: Tereshko Yan , Dal Bello Simone , Belgrado Enrico , Di Lorenzo Cherubino , Pittino Alice , Filippi Francesca , Valdemarin Francesca , Lettieri Christian , Gigli Gian Luigi , Nilo Annacarmen , Pellitteri Gaia , Merlino Giovanni , Valente Mariarosaria
Published: October 2024

Aims: The aim of this study is to assess the sleep quality and daytime sleepiness improvement in chronic migraineurs after 6 months of a 2:1 KD (ketogenic diet) and LGID (low-glycemic-index diet). Methods: Twenty-six patients underwent 2:1 KD (11 patients) and LGID (15 patients). PSQI (Pittsburgh sleep quality index) and ESS (Epworth sleepiness scale) were administered at the baseline and the 3-month and 6-month follow-up. MIDAS (Migraine Disability Assessment), HIT-6 (Headache Impact Test 6), migraine frequency (migraine days per month), migraine intensity, BMI (Body Mass Index), FM (Fat Mass), and FFM (Fat-Free Mass) were also assessed. Results: PSQI (F1.544, 38.606 = 7.250; p = 0.004), ESS (F1.988, 49.708 = 9.938; p < 0.001), HIT-6 (F1.432, 35.805 = 12.693; p < 0.001), migraine frequency (F1.522, 38.041 = 23.070; p < 0.001), migraine intensity (F1.949, 48.721 = 18.798; p < 0.001), BMI (F1.274, 31.857 = 38.191; p < 0.001), and FM (F1.245, 31.134 = 45.487; p < 0.001) improved significantly. The MIDAS (F1.005, 25.121 = 3.037; p = 0.093) and the FMM (F1.311, 32.784 = 1.741; p = 0.197) did not improve significantly. The ESS (p = 0.712) and PSQI (p = 0.776) data at 3-month and 6-month follow-ups did not differ significantly, as well as for migraine frequency, migraine intensity, BMI, FM, and HIT-6. A mild correlation emerged between the mean FM and mean ESS reduction during the 6 months (r = 0.497, p = 0.010). Conclusions: Six months of LGID and 2:1 KD can improve sleep quality and daytime sleepiness in patients with chronic migraine. The effectiveness on migraine, sleep quality, and daytime sleepiness does not differ significantly between the 3-month and 6-month follow-up periods.
Chronic Immune Sensory Polyradiculopathy (CISP): A Systematic Review of the Literature
Authors: Singhal Saurabh , Khanna Rahul , Surendranath Anudeep , Chhabra Jayksh , Thakkar Vismay , Gupta Rajesh
Published: October 2024

Chronic immune sensory polyradiculopathy (CISP) is a rare inflammatory immune disorder affecting the nervous system, primarily targeting the proximal sensory nerve roots. The condition was first described by Sinreich in 2004. We conducted a systematic review of CISP cases published on PubMed to identify common clinical presentations, along with neurophysiological, radiological, cerebrospinal fluid (CSF), and other findings. Our review included a total of 22 patients from 8 articles. Many patients presented with gait difficulties and sensory ataxia and were found to have normal nerve conduction studies (NCS) and electromyography (EMG) but exhibited characteristic abnormalities in somatosensory evoked potentials (SSEP), elevated CSF protein levels, thickened nerve roots on contrast-enhanced lumbar spine MRIs, and histological changes on nerve root biopsies. Clinical improvement was observed following treatment with steroids and/or intravenous immunoglobulin (IVIG). The study concluded that while CISP is rare, it is an important clinical entity to consider, as accurate diagnosis and appropriate treatment can lead to significant improvements in neurological symptoms and disabilities.
Effects of a Functional Cone Mushroom (Termitomyces fuliginosus) Protein Snack Bar on Cognitive Function in Middle Age: A Randomized Double-Blind Placebo-Controlled Trial
Authors: Muchimapura Supaporn , Thukham-mee Wipawee , Tong-un Terdthai , Sangartit Weerapon , Phuthong Sophida
Published: October 2024

Background: Due to the rising prevalence of cognitive impairment in the middle-aged and elderly population, combined with consumer demand for functional foods to improve health and well-being. Objective: This study aimed to formulate a functional cone mushroom (Termitomyces fuliginosus) (FCM) protein snack bar and evaluate its amino acid profile, phytochemical contents, biological activity and impact on cognitive function. Methods: A total of 26 middle-aged male and female participants were randomized and divided into placebo, FCM1 and FCM2 groups. Continuous consumption was performed for 6 weeks. Demographic data, body composition, cognitive function and memory were evaluated at baseline and at the end of the study period (6 weeks). Results: The event-related potential (ERP) analysis results showed a significant increase in N100 and P300 amplitude at the Fz location in participants who consumed the functional cone mushroom protein snack bar at a dose of 1 g compared to the placebo group (p = 0.015). Additionally, subjects who consumed the functional cone mushroom protein snack bar at a dose of 2 g showed a significantly increased P300 amplitude and percent accuracy of numeric working memory (p = 0.048) compared to those in the placebo group (p = 0.044). The possible underlying mechanism may involve AChE and MAO suppression activity alongside antioxidant activity. Conclusions: These data suggest that FCM can improve cognitive function and memory and may be considered for use in natural supplementation products with possible health benefits.
Body Composition and Senescence: Impact of Polyphenols on Aging-Associated Events
Authors: Santos Tanila Wood dos , Pereira Quélita Cristina , Fortunato Isabela Monique , Oliveira Fabrício de Sousa , Alvarez Marisa Claudia , Ribeiro Marcelo Lima
Published: October 2024

Aging is a dynamic and progressive process characterized by the gradual accumulation of cellular damage. The continuous functional decline in the intrinsic capacity of living organisms to precisely regulate homeostasis leads to an increased susceptibility and vulnerability to diseases. Among the factors contributing to these changes, body composition—comprised of fat mass and lean mass deposits—plays a crucial role in the trajectory of a disability. Particularly, visceral and intermuscular fat deposits increase with aging and are associated with adverse health outcomes, having been linked to the pathogenesis of sarcopenia. Adipose tissue is involved in the secretion of bioactive factors that can ultimately mediate inter-organ pathology, including skeletal muscle pathology, through the induction of a pro-inflammatory profile such as a SASP, cellular senescence, and immunosenescence, among other events. Extensive research has shown that natural compounds have the ability to modulate the mechanisms associated with cellular senescence, in addition to exhibiting anti-inflammatory, antioxidant, and immunomodulatory potential, making them interesting strategies for promoting healthy aging. In this review, we will discuss how factors such as cellular senescence and the presence of a pro-inflammatory phenotype can negatively impact body composition and lead to the development of age-related diseases, as well as how the use of polyphenols can be a functional measure for restoring balance, maintaining tissue quality and composition, and promoting health.
Are Maternal Dietary Patterns During Pregnancy Associated with the Risk of Gestational Diabetes Mellitus? A Systematic Review of Observational Studies
Authors: Kyrkou Charikleia , Athanasiadis Apostolos P. , Chourdakis Michael , Kada Stefania , Biliaderis Costas G. , Menexes Georgios , Michaelidou Alexandra-Maria
Published: October 2024

Background/Objectives: Maternal nutritional status is a “key” contributor to Gestational Diabetes Mellitus (GDM). However, the role of maternal dietary patterns (DPs) during pregnancy remains poorly understood. Thus, we conducted a systematic review to assess associations between “a posteriori-derived” DPs and GDM. Methods: A systematic search was conducted in PubMed, ScienceDirect, Web of Science, and Scopus for cohort, cross-sectional, and case–control studies published until June 2024. A total of twenty-eight studies involving 39,735 pregnant women were included, and their quality was evaluated by the Newcastle–Ottawa Scale. The 91 identified DPs were classified into four categories: “Westernized”, “Nutritious”, “Plant-based”, and “Miscellaneous”. Results: Our findings do not reveal definitive associations between maternal DPs during pregnancy and GDM risk. Notably, “Westernized” DPs tended to be associated with an increased risk. However, a very small portion of patterns within this category exhibited protective associations. Conversely, “Nutritious” and “Plant-based” appear beneficial for GDM prevention in specific populations. The “Miscellaneous” category presented an almost equal distribution of DPs with both detrimental and protective associations, pinpointing the absence of a clear directional trend regarding GDM risk. Conclusions: The heterogeneity in findings can be attributed to geographic and sociocultural variations and methodological differences across studies. Thus, there is a need for more standardized research methodologies to provide more precise insights that will ultimately help develop effective and tailored dietary guidelines for GDM prevention.
An In Silico Approach to Discover Efficient Natural Inhibitors to Tie Up Epstein–Barr Virus Infection
Authors: Das Ayan , Mumu Mumtaza , Rahman Tanjilur , Sayeed Md Abu , Islam Md Mazharul , Alawneh John I. , Hassan Mohammad Mahmudul
Published: October 2024

Epstein–Barr virus (EBV), also known as human herpesvirus 4, is a member of the herpes virus family. EBV is a widespread virus and causes infectious mononucleosis, which manifests with symptoms such as fever, fatigue, lymphadenopathy, splenomegaly, and hepatomegaly. Additionally, EBV is associated with different lymphocyte-associated non-malignant, premalignant, and malignant diseases. So far, no effective treatment or therapeutic drug is known for EBV-induced infections and diseases. This study investigated natural compounds that inhibit EBV glycoprotein L (gL) and block EBV fusion in host cells. We utilised computational approaches, including molecular docking, in silico ADMET analysis, and molecular dynamics simulation. We docked 628 natural compounds against gL and identified the four best compounds based on binding scores and pharmacokinetic properties. These four compounds, with PubChem CIDs 4835509 (CHx-HHPD-Ac), 2870247 (Cyh-GlcNAc), 21206004 (Hep-HHPD-Ac), and 51066638 (Und-GlcNAc), showed several interactions with EBV gL. However, molecular dynamics simulations indicated that the protein–ligand complexes of CID: 4835509 (CHx-HHPD-Ac) and CID: 2870247 (Cyh-GlcNAc) are more stable than those of the other two compounds. Therefore, CIDs 4835509 and 2870247 (Cyh-GlcNAc) may be potent natural inhibitors of EBV infection. These findings can open a new way for effective drug design against EBV and its associated infections and diseases.
Intestinal Parasites and Hematological Parameters in Children Living in Ambatoboeny District, Madagascar
Authors: Richert Wanesa , Kołodziej Daria , Zarudzka Danuta , Kasprowicz Daniel , Świetlik Dariusz , Korzeniewski Krzysztof
Published: October 2024

Madagascar is one of the poorest countries in the world. The country’s extreme weather conditions, poor sanitation, and weak economy facilitate the spread of parasitic diseases. Infections with intestinal parasites are particularly dangerous for children because they can cause malnutrition and anemia, which, in turn, have a negative effect on children’s cognitive functions and physical development. The aim of the present study was to analyze the prevalence of intestinal parasites and to assess hematological parameters in a group of children living in northern Madagascar. The screening was conducted in May 2024 in the Clinique Medicale Beyzym in Manerinerina, the Ambatoboeny district. It involved a sample of 208 children aged 0–17 years. Single stool samples were collected from all study participants. The samples were fixed in SAF fixative and then transported from Africa to Europe for further diagnostics at the Department of Epidemiology and Tropical Medicine, the Military Institute of Medicine–the National Research Institute in Poland. First, the samples were analyzed by light microscopy methods using three different diagnostic techniques (direct smear, decantation with distilled water, and the Fülleborn method). Next, they were tested by molecular biology methods (real-time PCR). Blood samples for the assessment of hematological parameters were collected at the healthcare center in Madagascar. The prevalence of intestinal parasites in the study sample was 61.5%. Helminths were found in 15.2% of the investigated children, and Giardia intestinalis (20.5%) was found to be the most prevalent parasite in the study population. Most infections were caused by potentially pathogenic stramenopila Blastocystis spp. (32.0%). Mean Hb, HCT, MCV, MCH, and MCHC levels in the study participants were below normal values. However, no correlation was found between the presence of a parasitic infection and low hematological parameters, which are a clinical sign of anemia. High rates of infections with intestinal parasites in children living in northern Madagascar support the necessity to introduce long-term preventive measures, which would limit the spread of parasitic diseases in the Malagasy population. Low hematological parameters in non-infected children may be indicative of persistent malnutrition or infection with other parasites, e.g., malaria or schistosomiasis.
The Effect of Biologic Agents on Steatotic Liver Disease in Patients with Inflammatory Bowel Disease: A Prospective, Open-Label Comparative Trial
Authors: Papaefthymiou Apostolis , Sarrou Styliani , Pateras Konstantinos , Vachliotis Ilias D. , Agrotis Georgios , Sgantzou Ioanna-Konstantina , Perifanos Georgios , Kapsoritakis Andreas , Speletas Matthaios , Vlychou Marianna , Dalekos George N. , Potamianos Spyros , Goulas Antonis , Kountouras Jannis , Polyzos Stergios A.
Published: October 2024

Background: Biologic agents used in patients with inflammatory bowel diseases (IBD) may influence the pathophysiology of coexistent metabolic-dysfunction associated steatotic liver disease (MASLD). This study primarily aimed to evaluate the six-month effect of infliximab or vedolizumab vs. no biologics on presumed hepatic steatosis in patients with IBD. Secondary endpoints were their effect on hepatic fibrosis and parameters related to hepatic metabolism. Methods: This prospective, non-randomized, controlled trial assigned adult bio-naïve patients with IBD into three groups: infliximab, vedolizumab, or controls (receiving no biologic). The baseline was the time of the initiation of biologic agents and the endpoint six months later. Hepatic steatosis was evaluated with transabdominal ultrasonography (Hamaguchi score), whereas controlled attenuation parameter (CAP), fatty liver index (FLI), and hepatic steatosis index (HSI) were used as surrogates. Hepatic fibrosis was evaluated with liver stiffness (LS), fibrosis-4 index (FIB-4), and nonalcoholic fatty liver disease (NAFLD) fibrosis score. Results: Sixty-six patients were assigned to infliximab (n = 26), vedolizumab (n = 14), or control (n = 26); At the endpoint, the Hamaguchi score, CAP, FLI, and HSI were not different between groups. LS was not different between groups; however, FIB-4 was increased within all groups, and NAFLD fibrosis score was increased within infliximab and control groups, without significant biologic × time interactions. Conclusions: No positive or adverse effect of infliximab or vedolizumab vs. no biologic agents was shown on presumed hepatic steatosis in patients with IBD, who have not been previously exposed to biologic agents. Although no effect of both biologic agent on LS, a slight but significant increase in FIB-4 and NAFLD fibrosis score warrants further studying.
Recent Advances in Designing Adeno-Associated Virus-Based Vaccines Against Viral Infections
Authors: Mnyandu Njabulo , Jacobs Ridhwaanah , Arbuthnot Patrick , Maepa Mohube Betty
Published: October 2024

Over 80% of the world’s deadliest pandemics are caused by viral infections, and vaccination remains the most effective way to prevent these infections from spreading. Since the discovery of the first vaccine over two centuries ago, several vaccine design technologies have been developed. Next-generation vaccines, based on mRNA and viral vector technologies, have recently emerged as alternatives to traditional vaccines. Adenoviral vector-based vaccines against coronavirus disease 2019 have demonstrated a more sustained antibody response as compared to mRNA vaccines. However, this has not been without complications, with a few cases of severe adverse events identified in vaccinated individuals, and the underlying mechanism is the subject of intense investigation. Adeno-associated viral vectors induce a weaker cellular immune response compared to adenoviral vectors, and it is mainly for this reason that there has been a diminished interest in exploring them as a vaccine platform until recently. This review will discuss recent developments and the potential of adeno-associated viral vectors as anti-viral vaccines.
Improved Malaria Therapy with Cationic Nanocapsules Demonstrated in Plasmodium berghei-Infected Rodents Using Whole Blood Surrogate Population PK/PD Modeling
Authors: Maciel Tamara Ramos , Funguetto-Ribeiro Ana Claudia , Olivo Laura Ben , Teixeira Flávia Elizabete Guerra , Pacheco Camila de Oliveira , Araujo Bibiana Verlindo de , Haas Sandra Elisa
Published: October 2024

Objectives: Investigating how nanoparticle systems interact in whole blood (WB) is critical to evaluating the effectiveness of malaria therapy. Methods: We decided to establish a pharmacokinetic/pharmacodynamic (PK/PD) model of the quinine population in WB using Plasmodium berghei-infected mice, with a subsequent model comparison for nanocapsules coated with polysorbate (NCP80) or prepared with Eudragit® RS (NCEUD). The WB quinine population pharmacokinetic model in rats was developed using plasma and partition coefficients for rat erythrocytes. Mouse WB quinine population PK/PD modeling was developed using allometrically scaled literature-free mouse quinine pharmacokinetic data and covariate values to obtain a WB population pharmacokinetic model for quinine and nanocapsules in mice. This allowed for PK/PD modeling of the quinine population with the WB concentration and parasitemia data in mice. All models were built in NONMEN. Results: The WB quinine concentration profiles in rats were characterized using a two-compartment model. Nanoencapsulation reduced clearance and central compartment volume and increased peripherical compartimental volume. A maximum effect model described the PK/PD of the quinine WB population in mice, demonstrating that NCEUD enhances the antimalarial effect. Conclusions: Quinine WB is a good surrogate for describing the response to exposure in malaria. NCEUD outperformed NCP80 and free quinine, suggesting that cationic surfaces improve the potential for treating malaria.
Unveiling the Larvicidal Potential of Golpar (Heracleum persicum Desf. ex Fisch.) Essential Oil and Its Main Constituents on Aedes and Anopheles Mosquito Vectors
Authors: Ferrati Marta , Baldassarri Cecilia , Rossi Paolo , Favia Guido , Benelli Giovanni , De Fazi Livia , Morshedloo Mohammad Reza , Quassinti Luana , Petrelli Riccardo , Spinozzi Eleonora , Maggi Filippo
Published: October 2024

Natural products are thoroughly studied as valuable alternatives to synthetic insecticides. Heracleum persicum Desf. ex Fisch. (Apiaceae), commonly known as Golpar, is an Iranian medicinal plant largely employed as a spice, which has previously revealed insecticidal potential. The chemical composition of H. persicum essential oil (EO) was investigated by GC-MS and was mainly dominated by hexyl butyrate (36.1%) and octyl acetate (23.7%). The EO and its main esters were tested on three mosquito species. Aedes aegypti (L.) larvae were the most sensitive to all tested products. Lethal concentrations (LC50) of 59.09, 53.59, and 47.05 ppm were recorded for the EO, hexyl butyrate, and octyl acetate, respectively. Aedes albopictus (Skuse) and Anopheles gambiae Giles demonstrated comparable sensitivity to the EO, with LC50 values of 102.97 and 97.91 ppm, respectively, whereas the isolated constituents appeared more active on An. gambiae (LC50 of hexyl butyrate and octyl acetate of 70.97 and 60.71 ppm, respectively) with respect to Ae. albopictus (LC50 of hexyl butyrate and octyl acetate of 85.40 and 91.38 ppm, respectively). Low toxicity was registered for both EO and single components against human embryonic kidney (HEK293) cells. Overall, the H. persicum EO, hexyl butyrate, and octyl acetate could be further considered for larvicide development.
Comparative Study on the Response of Hyssop (Hyssopus officinalis L.), Salvia (Salvia officinalis L.), and Oregano (Origanum vulgare L.) to Drought Stress Under Foliar Application of Selenium
Authors: Skrypnik Liubov , Maslennikov Pavel , Antipina Maria , Katserov Dmitriy , Feduraev Pavel
Published: October 2024

Drought is one of the most important abiotic factors limiting plant productivity. Although the aromatic plants of the Lamiaceae family often grow in arid regions, drought tolerance varies greatly among the different species of this family. The effect of induced drought stress can be reduced by the application of selenium. The current study aims to compare the growth and biochemical responses of three species of the Lamiaceae family (hyssop, salvia, and oregano) to drought stress and the possibility of reducing the effect of stress in these plants by foliar treatment with selenium. Drought stress reduced the fresh and dry biomass of hyssop (by 35% and 15%), salvia (by 45% and 41%), and oregano (by 51% and 32%). Se treatment did not affect the growth of plants under drought stress, but it improved relative water content in hyssop and salvia under moderate drought conditions. A reduction in the content of chlorophyll a and chlorophyll b (in hyssop and salvia). In addition, an increase in the content of hydrogen peroxide (in oregano and salvia), malondialdehyde, and proline in plants cultivated under drought conditions was observed. Se treatment led to reduced levels of hydrogen peroxide and malondialdehyde, along with an increase in chlorophyll a content (in hyssop and oregano) and proline content. The response of the antioxidant system depended on the plant species. Hyssop exhibited a significant increase in glutathione peroxidase, superoxide dismutase, and peroxidase activities. Oregano showed enhanced catalase activity. Salvia experienced a sharp increase in ascorbic acid content. Se treatment stimulated the accumulation of phenolic compounds and increased glutathione peroxidase activity in all studied species.
Optimizing Peanut (Arachis hypogaea L.) Production: Genetic Insights, Climate Adaptation, and Efficient Management Practices: Systematic Review
Authors: Gelaye Yohannes , Luo Huaiyong
Published: October 2024

Peanut production plays a crucial role in global food security, particularly in developing countries, where it provides essential nutrition and income. This paper examines the optimization of peanut production through genetic advancements, climate adaptation strategies, and sustainable practices. The primary objective is to increase yields by addressing challenges related to climate change, pests, and resource constraints. Globally, peanut production is hindered by rising temperatures, irregular rainfall, and declining soil quality, impacting both yield and quality. Developing countries, especially in Africa and Asia, face additional challenges, such as limited access to advanced agricultural technologies, inadequate infrastructure, and insufficient support for smallholder farmers. The vital issues include genetic vulnerabilities to pests, climate stress, and inefficient water use. Recent genetic research has provided insights into breeding more resilient, drought-resistant varieties, offering hope for improving yields, despite environmental challenges. The adoption of climate adaptation strategies, precision farming, and integrated pest management is essential for boosting productivity. These, along with optimized irrigation and nutrient management, have significantly impacted peanut production in resource-limited settings. Additionally, drought-resistant varieties have proven crucial, enabling farmers to increase resilience and yields in areas facing climate stress. In conclusion, optimizing peanut production requires continued investment in genetic advancements, infrastructure, and sustainable practices. Future efforts should focus on improving climate adaptation and sustainable farming techniques for long-term success.
DDMMO Website Quality, Destination Image and Intention to Use Metaverse Platforms
Authors: Deirmentzoglou Georgios A. , Vlassi Eirini , Anastasopoulou Eleni E.
Published: October 2024

Background/Objectives: Destination Development, Management, and Marketing Organizations (DDMMOs) have the power to influence perceptions and behaviors regarding both actual and virtual travel in the rapidly changing landscape of digital environments. Within newly emerging Metaverse platforms, their websites can serve as critical touchpoints that enhance destination attractiveness and enable visitors to engage in valuable experiences. In this vein, this research attempts to determine if DDMMO website quality and destination image can influence users’ intention to virtually visit a place by using a Metaverse platform. Methods: Users who navigated a European DDMMO website were asked to fill out a self-administered questionnaire, and 318 responses were collected. Then, structural equation modeling (SEM) was used to test the research hypothesis. Results: The results show that website interactivity and affective destination image had a direct positive impact on a user’s intention to use the Metaverse platform. Furthermore, indirect impacts of website design and usefulness and cognitive destination image were detected. Conclusions: DDMMOs and destination marketing experts can gain valuable insights from the outcomes of this research. Thus, focusing on the aforementioned website features can help them enhance destination image and attract users to their Metaverse platforms.
Detecting Multi-Scale Defects in Material Extrusion Additive Manufacturing of Fiber-Reinforced Thermoplastic Composites: A Review of Challenges and Advanced Non-Destructive Testing Techniques
Authors: Ashebir Demeke Abay , Hendlmeier Andreas , Dunn Michelle , Arablouei Reza , Lomov Stepan V. , Di Pietro Adriano , Nikzad Mostafa
Published: October 2024

Additive manufacturing (AM) defects present significant challenges in fiber-reinforced thermoplastic composites (FRTPCs), directly impacting both their structural and non-structural performance. In structures produced through material extrusion-based AM, specifically fused filament fabrication (FFF), the layer-by-layer deposition can introduce defects such as porosity (up to 10–15% in some cases), delamination, voids, fiber misalignment, and incomplete fusion between layers. These defects compromise mechanical properties, leading to reduction of up to 30% in tensile strength and, in some cases, up to 20% in fatigue life, severely diminishing the composite’s overall performance and structural integrity. Conventional non-destructive testing (NDT) techniques often struggle to detect such multi-scale defects efficiently, especially when resolution, penetration depth, or material heterogeneity pose challenges. This review critically examines manufacturing defects in FRTPCs, classifying FFF-induced defects based on morphology, location, and size. Advanced NDT techniques, such as micro-computed tomography (micro-CT), which is capable of detecting voids smaller than 10 µm, and structural health monitoring (SHM) systems integrated with self-sensing fibers, are discussed. The role of machine-learning (ML) algorithms in enhancing the sensitivity and reliability of NDT methods is also highlighted, showing that ML integration can improve defect detection by up to 25–30% compared to traditional NDT techniques. Finally, the potential of self-reporting FRTPCs, equipped with continuous fibers for real-time defect detection and in situ SHM, is investigated. By integrating ML-enhanced NDT with self-reporting FRTPCs, the accuracy and efficiency of defect detection can be significantly improved, fostering broader adoption of AM in aerospace applications by enabling the production of more reliable, defect-minimized FRTPC components.
A Comprehensive Review on the Tribological Evaluation of Polyether Ether Ketone Pristine and Composite Coatings
Authors: Seenath Amal A. , Baig M. M. A. , Katiyar Jitendra Kumar , Mohammed Abdul Samad
Published: October 2024

Polymer coatings have gained a lot of attention in the recent past because of their ability to be easily coated on complex shapes, their low cost, and their ability to reduce friction as compared to other materials. Polyether ether ketone (PEEK) is one such high-performance polymer that has gained significant attention in recent years due to its exceptional mechanical properties, chemical resistance, and thermal stability making it a prominent candidate for applications in industries. However, PEEK in its pristine form exhibits poor wear resistance with a moderate coefficient of friction (0.30–0.38). Many attempts have been made by several researchers to improve its wear resistance and lower the COF by developing composite coatings. Hence, in this review, we aim to summarize and present in detail the tribological evaluation of pristine PEEK and PEEK composite coatings by discussing the various methods adopted by the researchers to improve the properties of PEEK, the different types of reinforcements and various dispersion techniques used to develop PEEK composite coatings. By consolidating and analyzing the existing body of knowledge, we also aim to offer valuable insights into the development of more durable, high-performance PEEK nanocomposite coatings for a broad range of tribological applications.
Dual Antimicrobial Activity of HTCC and Its Nanoparticles: A Synergistic Approach for Antibacterial and Antiviral Applications Through Combined In Silico and In Vitro Studies
Authors: Dhlamini Khanyisile S. , Selepe Cyril T. , Ramalapa Bathabile , Cele Zamani , Malatji Kanyane , Govender Krishna K. , Tshweu Lesego , Ray Suprakas Sinha
Published: October 2024

N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), a quaternized chitosan derivative, has been shown to exhibit a broad spectrum of antimicrobial activity, especially against bacteria and enveloped viruses. Despite this, molecular docking studies showing its atomic-level mechanisms against these microorganisms are scarce. Here, for the first time, we employed molecular docking analyses to investigate the potential antibacterial activity of HTCC against Staphylococcus aureus and its antiviral activity against human immunodeficiency virus 1 (HIV-1). According to the findings, HTCC exhibited promising antibacterial activity with high binding affinities; however, it had limited antiviral activity. To validate these theoretical outcomes, experimental studies were conducted. Different derivatives of HTCC were synthesized and characterized using NMR, XRD, FTIR, and DLS. The in vitro assays validated the potent antibacterial efficacy of HTCC against S. aureus, whereas the antiviral studies did not show good antiviral activity. However, our research also revealed a promising avenue for further exploration of the antimicrobial activity of HTCC nanoparticles (NPs), since, thus far, no studies have been conducted to show the antiviral activity of HTCC NPs against HIV-1. The nanosized HTCC exhibited superior antiviral performance compared to the parent polymers, with complete (100%) inhibition of HIV-1 viral activity at the highest tested concentration (0.33 mg/mL).
Isolation and Characterization of Novel Cellulose Micro/Nanofibers from Lygeum spartum Through a Chemo-Mechanical Process
Authors: Ahmima Sabrina , Naar Nacira , Jędrzejczak Patryk , Klapiszewska Izabela , Klapiszewski Łukasz , Jesionowski Teofil
Published: October 2024

Recent studies have focused on the development of bio-based products from sustainable resources using green extraction approaches, especially nanocellulose, an emerging nanoparticle with impressive properties and multiple applications. Despite the various sources of cellulose nanofibers, the search for alternative resources that replace wood, such as Lygeum spartum, a fast-growing Mediterranean plant, is crucial. It has not been previously investigated as a potential source of nanocellulose. This study investigates the extraction of novel cellulose micro/nanofibers from Lygeum spartum using a two-step method, including both alkali and mechanical treatment as post-treatment with ultrasound, as well as homogenization using water and dilute alkali solution as a solvent. To determine the structural properties of CNFs, a series of characterization techniques was applied. A significant correlation was observed between the Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) results. The FTIR results revealed the elimination of amorphous regions and an increase in the energy of the H-bonding modes, while the XRD results showed that the crystal structure of micro/nanofibers was preserved during the process. In addition, they indicated an increase in the crystallinity index obtained with both methods (deconvolution and Segal). Thermal analysis based on thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) confirmed improvement in the thermal properties of the isolated micro/nanofibers. The temperatures of maximum degradation were 335 °C and 347 °C. Morphological analysis using a scanning electron microscope (SEM) and atomic force microscope (AFM) showed the formation of fibers along the axis, with rough and porous surfaces. The findings indicate the potential of Lygeum spartum as a source for producing high-quality micro/nanofibers. A future direction of study is to use the cellulose micro/nanofibers as additives in recycled paper and to evaluate the mechanical properties of the paper sheets, as well as investigate their use in smart paper.
Korean Buddhism in the Era of ‘Spiritual, but Not Religious’: Adapting to Contemporary Society
Authors: Somers Brian D.
Published: October 2024

The shift toward modernity has come with many changes that affect religion. This article investigates some of those changes with the aim of showing how Korean Buddhism is adjusting to contemporary spirituality. The article begins with a consideration of the term ‘spiritual, but not religious’ (SBNR), a relatively new designation that indicates the shift a growing number of people are taking away from organized religious institutes towards beliefs and practices that they find more relevant and meaningful. To better understand SBNRs, the research of Mercadante is emphasized. The second half of this article aims to consider Korean Buddhism in the context of modern spirituality. Using Mercadante’s framework, which distinguishes between five types of SBNRs (dissenters, causals, explorers, seekers, and immigrants), an inquiry is carried out into each of these categories to show some of the ways in which Korean Buddhism is engaging with the spiritual and emotional concerns of modern, secular society. Thus, this work aims to show the direction Korean Buddhism is headed in and how it is relevant to modern, spiritual inclinations.
Methodology for Integrated Design Optimization of Actuation Systems for Exoskeletons
Authors: Greve Daniel , Kreischer Christian
Published: October 2024

The engineering of actuation systems for active exoskeletons presents a significant challenge due to the stringent demands for mass reduction and compactness, coupled with complex specifications for actuator dynamics and stroke length. This challenge is met with a model-based methodology. Models for human body, exoskeleton and parametric actuation systems are derived and coupled. Beginning with an inverse dynamics human body simulation, loads in human joints are estimated, and the corresponding support torques are derived. Under the assumption of a control law ensuring these support torques, an optimization problem is stated to determine actuation system parameters such as the number of stator coils and number of battery cells. Lastly, results from the optimization are validated using sophisticated models. The methodology is applied to an exemplary exoskeleton and compared to an approach derived from previous studies.