bluuebunny/crossref_metadata_2025 huggingface dataset:
Garbage Patches and Their Environmental Implications in a Plastisphere
Authors: Leal Filho Walter , Hunt Julian , Kovaleva Marina
Published: November 2021

This Communication reports on the increases in the sizes of garbage patches, and their environmental implications, outlining the dimensions of what is a growing problem connected with the “plastisphere”. The paper presents some data on the distribution of garbage patches in the world’s oceans and makes some predictions on future growth, which is partly associated with the future increases in worldwide plastics production. The findings demonstrate that the size of the main garbage patches is increasing, posing a threat to the environment and marine life. The paper urges for better plastic waste management to prevent it from reaching the oceans, along with concerted actions in respect of plastic collection and cleaning up the oceans, which may include new technological solutions.
Performance of the Car Carrier Shipping Sector in the Iberian Peninsula under the COVID-19 Scenario
Authors: Esteve-Pérez Jerónimo , Gutiérrez-Romero José Enrique , Mascaraque-Ramírez Carlos
Published: November 2021

The Iberian Peninsula represents the second European producer and the eighth world producer of vehicles in 2020. The pandemic of SARS-CoV2 introduced severe challenges for the worldwide population and for the industrial production and supply chains. The car carrier shipping sector has not been studied in depth in the Maritime Transportation and Port Logistics literature. This research pays special attention to the performance of this traffic in the Iberian Peninsula in the pre-pandemic era and under COVID-19 pandemic conditions, in which seven ports with car-carrier ship traffic in the Iberian Peninsula are analyzed. First, a dynamic portfolio analysis about how the COVID-19 pandemic affected the evolution of competitive positions of Iberian Peninsula ports is performed. Second, studies of the seasonality patterns of vehicle movements in ports of the Iberian Peninsula were carried out using time series of the periods from 2012 to 2019 and from 2012 to 2020. The Seasonal Variation Index (SVI) was employed to determine the seasonality of vehicle traffic in the periods considered and analyses were performed independently for both embarking and disembarking traffic. Important conclusions are revealed, e.g., during a year of COVID-19, the seven ports had decreased vehicle movements for disembarking traffic and only one port increased the traffic for embarking traffic. Furthermore, COVID-19 introduced important changes in the seasonality patterns of vehicle movements during the first months of the pandemic.
External Shocks and Volatility Overflow among the Exchange Rate of the Yen, Nikkei, TOPIX and Sectoral Stock Indices
Authors: Sultonov Mirzosaid
Published: November 2021

In this paper, we examined the changes in volatility overflow among the exchange rate of the Japanese yen (JPY), the Nikkei Stock Average (Nikkei), the Tokyo Stock Price Index (TOPIX) and the TOPIX sectoral indices for the period of 10 February 2016 to 24 March 2017. We employed the exponential generalised autoregressive conditional heteroscedasticity (EGARCH) model, the cross-correlation function, and the daily logarithmic returns of JPY, Nikkei, TOPIX and the TOPIX components with a weight of 5% and more in estimations (banks, chemicals, electric appliances, information and communication, machinery and transportation equipment indices). The findings highlighted causality in variance (volatility spillover) among the variables. We revealed that volatility could also spread indirectly among the variables (from one variable to another through a third variable). We demonstrated how the impact of news about the results of the Brexit referendum (BR) and the United States presidential election (USE) in 2016 might spread among the variables indirectly within a week.
Auditory and Acoustic Evidence for Palatalization of the Nasal Consonant in Cairene Arabic
Authors: Sokhey Navdeep
Published: November 2021

This paper introduces the palatalized nasal [nʲ] as an allophonic realization of coronal /n/ in Cairene Arabic. The palatalized variants of the phonemes previously described in acoustic and sociolinguistic terms include the alveolar stops [t, d] and their pharyngealized counterparts [tˤ, dˤ], which can be palatalized preceding the high, front vowel [i:]. While previous studies have anecdotally noted that the coronal nasal /n/ can undergo palatalization in the same environment, this variant has not been systematically investigated. Focusing on syllable-final /-ni:/ segments, I first use auditory measures to show that the palatalized variant occurs with some regularity (~50%) in the read speech of seven speakers of Cairene Arabic. Then, I provide acoustic evidence that this perceived difference significantly correlates with the difference in F2 values taken from the onset and midpoint of the vowel following the nasal consonant. There is also evidence of a lexical effect, such that borrowings exhibit less palatalization than non-borrowings. This study contributes data for the unexamined Cairene nasal and supports the likelihood of palatalization of coronals at the typological level.
Process Parameter Optimization of a Polymer Derived Ceramic Coatings for Producing Ultra-High Gas Barrier
Authors: Channa Iftikhar Ahmed , Shah Aqeel Ahmed , Rizwan Muhammad , Makhdoom Muhammad Atif , Chandio Ali Dad , Shar Muhammad Ali , Mahmood Asif
Published: November 2021

Silica is one of the most efficient gas barrier materials, and hence is widely used as an encapsulating material for electronic devices. In general, the processing of silica is carried out at high temperatures, i.e., around 1000 °C. Recently, processing of silica has been carried out from a polymer called Perhydropolysilazane (PHPS). The PHPS reacts with environmental moisture or oxygen and yields pure silica. This material has attracted many researchers and has been widely used in many applications such as encapsulation of organic light-emitting diodes (OLED) displays, semiconductor industries, and organic solar cells. In this paper, we have demonstrated the process optimization of the conversion of the PHPS into silica in terms of curing methods as well as curing the environment. Various curing methods including exposure to dry heat, damp heat, deep UV, and their combination under different environments were used to cure PHPS. FTIR analysis suggested that the quickest conversion method is the irradiation of PHPS with deep UV and simultaneous heating at 100 °C. Curing with this method yields a water permeation rate of 10−3 g/(m2⋅day) and oxygen permeation rate of less than 10−1 cm3/(m2·day·bar). Rapid curing at low-temperature processing along with barrier properties makes PHPS an ideal encapsulating material for organic solar cell devices and a variety of similar applications.
High-Impact Polystyrene Reinforced with Reduced Graphene Oxide as a Filament for Fused Filament Fabrication 3D Printing
Authors: Sieradzka Marta , Fabia Janusz , Biniaś Dorota , Graczyk Tadeusz , Fryczkowski Ryszard
Published: November 2021

Graphene and its derivatives, such as graphene oxide (GO) or reduced graphene oxide (rGO), due to their properties, have been enjoying great interest for over two decades, particularly in the context of additive manufacturing (AM) applications in recent years. High-impact polystyrene (HIPS) is a polymer used in 3D printing technology due to its high dimensional stability, low cost, and ease of processing. However, the ongoing development of AM creates the need to produce modern feedstock materials with better properties and functionality. This can be achieved by introducing reduced graphene oxide into the polymer matrix. In this study, printable composite filaments were prepared and characterized in terms of morphology and thermal and mechanical properties. Among the obtained HIPS/rGO composites, the filament containing 0.5 wt% of reduced graphene oxide had the best mechanical properties. Its tensile strength increased from 19.84 to 22.45 MPa, for pure HIPS and HIPS-0.5, respectively. Furthermore, when using the HIPS-0.5 filament in the printing process, no clogging of the nozzle was observed, which may indicate good dispersion of the rGO in the polymer matrix.
Thermal Properties of Calcium Sulphoaluminate Cement as an Alternative to Ordinary Portland Cement
Authors: Gołaszewska Małgorzata , Klemczak Barbara , Gołaszewski Jacek
Published: November 2021

This paper presents the results of research into the heat of hydration and activation energy of calcium sulphoaluminate (CSA) cement in terms of the dependence on curing temperature and water/cement ratio. Cement pastes with water/cement ratios in the range of 0.3–0.6 were tested by isothermal calorimetry at 20 °C, 35 °C and 50 °C, with the evolved hydration heat and its rate monitored for 168 h from mixing water with cement. Reference pastes with ordinary Portland cement (OPC) were also tested in the same range. The apparent activation energy of CSA and OPC was determined based on the results of the measurements. CSA pastes exhibited complex thermal behaviour that differed significantly from the thermal behaviour of ordinary Portland cement. The results show that both the w/c ratio and elevated temperature have a meaningful effect on the heat emission and the hydration process of CSA cement pastes. The determined apparent activation energy of CSA revealed its substantial variability and dependence, both on the w/c ratio and the curing temperature.
Experimental Study of the Impact of Glass Beads on Adhesive Joint Strength and Its Failure Mechanism
Authors: Santos João P. J. R. , Marques Eduardo A. S. , Carbas Ricardo J. C. , Gilbert Frida , da Silva Lucas F. M.
Published: November 2021

The use of modern structural adhesives provides a lightweight, practical, and high strength joining methodology, which is increasingly being adopted in the automotive and aeronautical sectors, among many others. However, the strict mechanical performance standards that must be met in these applications require a constant search for ways of improving the adhesives’ behavior, which has led to the growing use of reinforcements as a way of improving the capabilities of bonded joints. The aim of this work was, thus, to analyze how the addition of inorganic fillers to the adhesive layer affects a joint’s strength and its failure mechanism. To this end, single lap joint specimens with mild steel and high strength steel substrates were tested, at quasi-static speeds, and with different amounts of glass microspheres reinforcing two different structural adhesives. The experimental results indicated that the addition of glass particles reduced the joint performance for both substrates under study. Furthermore, the failure pattern was found to evolve from adhesive failure to a cohesive type of failure as the amount of glass particles present in the adhesive was increased.
Influence of Asphalt Emulsion Inclusion on Fly Ash/Hydrated Lime Alkali-Activated Material
Authors: Bualuang Thanon , Jitsangiam Peerapong , Suwan Teewara , Rattanasak Ubolluk , Tangchirapat Weerachart , Thongmunee Suriyah
Published: November 2021

Supplementary cementitious materials have been widely used to reduce the greenhouse gas emissions caused by ordinary Portland cement (OPC), including in the construction of road bases. In addition, the use of OPC in road base stabilization is inefficient due to its moisture sensitivity and lack of flexibility. Therefore, this study investigates the effect of hybrid alkali-activated materials (H-AAM) on flexibility and water prevention when used as binders while proposing a new and sustainable material. A cationic asphalt emulsion (CAE) was applied to increase this cementless material’s resistance to moisture damage and flexibility. The physical properties and structural formation of this H-AAM, consisting of fly ash, hydrated lime, and sodium hydroxide, were examined. The results revealed that the addition of CAE decreased the material’s mechanical strength due to its hindrance of pozzolanic reactions and alkali activations. This study revealed decreases in the cementitious product’s peak in the x-ray diffraction analysis (XRD) tests and the number of tetrahedrons detected in the Fourier transform infrared spectroscopy analysis (FTIR) tests. The scanning electron microscope (SEM) images showed some signs of asphalt films surrounding hybrid alkali-activated particles and even some unreacted FA particles, indicating incomplete chemical reactions in the study material’s matrix. However, the H-AAM was still able to meet the minimum road base strength requirement of 1.72 MPa. Furthermore, the toughness and flexibility of the H-AAM were enhanced by CAE. Notably, adding 10% and 20% CAE by weight to the hybrid alkali-activated binder produced a significant advantage in terms of water absorption, which can be explained by its influence on the material’s consolidation of its matrices, resulting in significant void reductions. Hence, the outcomes of this study might reveal an opportunity for developing a new stabilizing agent for road bases with water-prevention properties and flexibility that remains faithful to the green construction material concept.
Theoretical and Experimental Research on Switching Optimization of No. 9 Single Turnout with 60-kg/m Rails
Authors: Wang Pu , Wang Shuguo , Zhao Zhenhua , Si Daolin
Published: November 2021

Based on the necessity of optimizing the structure of No. 9 single turnouts of 60-kg/m rails, we addressed the issues associated with existing turnout switching design methods. Based on finite element analysis, we established a refined calculation model for turnout switching. The model can determine the plane alignment of a switch rail separated from the stock rail based on the actual force acting upon the switch rail. The obtained plane alignment is consistent with the actual situation and is thus reliable. Based on the established turnout switching model, the minimum flangeway width and dynamics between the strokes of the first and second traction points under different conditions were analyzed by numerical simulations. Accordingly, we propose an optimized scheme that takes 160 mm and 85 mm as the stroke value for the first and second traction points, respectively. The scheme helps to meet the requirements for minimum flangeway width while making the deformation of the switch rail more even and therefore minimizing the traction power. Based on the proposed design, trial production and laying of the new No. 9 single turnout with a 60-kg/m rail was conducted, and a switching test was performed. The switching forces at the first and second traction points of the new No. 9 turnout were approximately 1200 and 2000 N, respectively, which were higher than those of existing No. 9 turnouts with 60-kg/m steel rails. Besides, the minimum flangeway width satisfies the requirement for safe vehicle passage with a safety margin of 3–5 mm. The test results proved the effectiveness of the proposed turnout switching design method and parameter optimization scheme.
A Note on the Boundedness of Doob Maximal Operators on a Filtered Measure Space
Authors: Chen Wei , Cui Jingya
Published: November 2021

Let M be the Doob maximal operator on a filtered measure space and let v be an Ap weight with 1<p<+∞. We try proving that ∥Mf∥Lp(v)≤p′[v]Ap1p−1∥f∥Lp(v), where 1/p+1/p′=1. Although we do not find an approach which gives the constant p′, we obtain that ∥Mf∥Lp(v)≤p1p−1p′[v]Ap1p−1∥f∥Lp(v), with limp→+∞p1p−1=1.
Agent Scheduling in Unrelated Parallel Machines with Sequence- and Agent–Machine–Dependent Setup Time Problem
Authors: Vázquez-Serrano Jesús Isaac , Cárdenas-Barrón Leopoldo Eduardo , Peimbert-García Rodrigo E.
Published: November 2021

Assignation-sequencing models have played a critical role in the competitiveness of manufacturing companies since the mid-1950s. The historic and constant evolution of these models, from simple assignations to complex constrained formulations, shows the need for, and increased interest in, more robust models. Thus, this paper presents a model to schedule agents in unrelated parallel machines that includes sequence and agent–machine-dependent setup times (ASUPM), considers an agent-to-machine relationship, and seeks to minimize the maximum makespan criteria. By depicting a more realistic scenario and to address this NP-hard problem, six mixed-integer linear formulations are proposed, and due to its ease of diversification and construct solutions, two multi-start heuristics, composed of seven algorithms, are divided into two categories: Construction of initial solution (designed algorithm) and improvement by intra (tabu search) and inter perturbation (insertions and interchanges). Three different solvers are used and compared, and heuristics algorithms are tested using randomly generated instances. It was found that models that linearizing the objective function by both job completion time and machine time is faster and related to the heuristics, and presents an outstanding level of performance in a small number of instances, since it can find the optimal value for almost every instance, has very good behavior in a medium level of instances, and decent performance in a large number of instances, where the relative deviations tend to increase concerning the small and medium instances. Additionally, two real-world applications of the problem are presented: scheduling in the automotive industry and healthcare.
Nutrients, Cognitive Function, and Brain Aging: What We Have Learned from Dogs
Authors: Pan Yuanlong
Published: November 2021

Due to a difference in genetics, environmental factors, and nutrition, just like in people, dogs age at different rates. Brain aging in people and dogs share similar morphological changes including irreversible cortical atrophy, cerebral amyloid angiopathy, and ventricular enlargement. Due to severe and irreversible brain atrophy, some aging dogs develop cognitive dysfunction syndrome (CDS), which is equivalent to dementia or Alzheimer’s disease (AD) in people. The risk factors and causes of CDS in dogs have not been fully investigated, but age, gender, oxidative stress, and deficiency of sex hormones appears to be associated with increased risk of accelerated brain aging and CDS in dogs. Both AD and CDS are incurable diseases at this moment, therefore more efforts should be focused on preventing or reducing brain atrophy and minimizing the risk of AD in people and CDS in dogs. Since brain atrophy leads to irreversible cognitive decline and dementia, an optimal nutritional solution should be able to not only enhance cognitive function during aging but also reduce irreversible brain atrophy. Up to now, only one nutritional intervention has demonstrated both cognition-enhancing benefits and atrophy-reducing benefits.
Challenging the Conventional Interpretation of HCMV Seronegativity
Authors: Waters Shelley , Lee Silvia , Irish Ashley , Price Patricia
Published: November 2021

The majority of adults in the world (around 83%) carry antibodies reactive with HCMV and are thought to retain inactive or latent infections lifelong. The virus is transmitted via saliva, so infection events are likely to be common. Indeed, it is hard to imagine a life without exposure to HCMV. From 45 seronegative individuals (13 renal transplant recipients, 32 healthy adults), we present seven cases who had detectable HCMV DNA in their blood and/or saliva, or a CMV-encoded homologue of IL-10 (vIL-10) in their plasma. One case displayed NK cells characteristic of CMV infection before her HCMV DNA became undetectable. In other cases, the infection may persist with seroconversion blocked by vIL-10. Future research should seek mechanisms that can prevent an individual from seroconverting despite a persistent HCMV infection, as HCMV vaccines may not work well in such people.
Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in Brassica
Authors: Roodi Davood , Millner James P. , McGill Craig R. , Johnson Richard D. , Hea Shen-Yan , Brookes Jenny J. , Glare Travis R. , Card Stuart D.
Published: November 2021

Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of Brassica, identified as Beauveria bassiana and Pseudogymnoascus pannorum, were inoculated into cultivars of three Brassica species—Brassica napus, Br. rapa and Br. oleracea. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant–endophyte associations formed between Brassica plants and Be. bassiana significantly inhibited phoma stem canker, a devastating disease of Brassica crops worldwide, caused by the fungal pathogen Leptosphaeria maculans. The novel association formed with P. pannorum significantly suppressed the amount of disease caused by L. maculans in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops.
Composition and Biological Activity of Vitis vinifera Winter Cane Extract on Candida Biofilm
Authors: Kodeš Zdeněk , Vrublevskaya Maria , Kulišová Markéta , Jaroš Petr , Paldrychová Martina , Pádrová Karolína , Lokočová Kristýna , Palyzová Andrea , Maťátková Olga , Kolouchová Irena
Published: November 2021

Vitis vinifera canes are waste material of grapevine pruning and thus represent cheap source of high-value polyphenols. In view of the fact that resistance of many pathogenic microorganisms to antibiotics is a growing problem, the antimicrobial activity of plant polyphenols is studied as one of the possible approaches. We have investigated the total phenolic content, composition, antioxidant activity, and antifungal activity against Candida biofilm of an extract from winter canes and a commercially available extract from blue grapes. Light microscopy and confocal microscopy imaging as well as crystal violet staining were used to quantify and visualize the biofilm. We found a decrease in cell adhesion to the surface depending on the concentration of resveratrol in the cane extract. The biofilm formation was observed as metabolic activity of Candida albicans, Candida parapsilosis and Candida krusei biofilm cells and the minimum biofilm inhibitory concentrations were determined. The highest inhibition of metabolic activity was observed in Candida albicans biofilm after treatment with the cane extract (30 mg/L) and blue grape extract (50 mg/L). The composition of cane extract was analyzed and found to be comparatively different from blue grape extract. In addition, the content of total phenolic groups in cane extract was three-times higher (12.75 gGA/L). The results showed that cane extract was more effective in preventing biofilm formation than blue grape extract and winter canes have proven to be a potential source of polyphenols for antimicrobial and antibiofilm treatment.
The Hydrothermal Alteration of the Cordón de Inacaliri Volcanic Complex in the Framework of the Hidden Geothermal Systems within the Pabelloncito Graben (Northern Chile)
Authors: Maza Santiago , Collo Gilda , Morata Diego , Cuña-Rodriguez Carolina , Taussi Marco , Renzulli Alberto
Published: November 2021

Detailed mineralogical analyses in areas with surface hydrothermal alteration zones associated with recent volcanism (<1 Ma) in the Central Andean Volcanic Zone could provide key information to unravel the presence of hidden geothermal systems. In the Cordón de Inacaliri Volcanic Complex, a geothermal field with an estimated potential of ~1.08 MWe·km−2 has been recently discovered. In this work, we focus on the hydrothermal alteration zones and discharge products of this area, with the aim to reconstruct the geological processes responsible for the space-time evolution leading to the geothermal records. We identified (1) discharge products associated with acid fluids that could be related to: (i) acid-sulfate alteration with alunite + kaolinite + opal CT + anatase, indicating the presence of a steam-heated blanket with massive fine-grained silica (opal-CT), likely accumulated in mud pots where the intersection of the paleowater table with the surface occurred; (ii) argillic alteration with kaolinite + hematite + halloysite + smectite + I/S + illite in the surrounding of the acid-sulfate alteration; and (2) discharge products associated with neutral-alkaline fluids such as: (i) discontinuous pinnacle-like silica and silica deposits with laterally developed coarse stratification which, together with remaining microorganisms, emphasize a sinter deposit associated with alkaline/freshwater/brackish alkaline-chlorine water bodies and laterally associated with (ii) calcite + aragonite deriving from bicarbonate waters. The scarce presence of relics of sinter deposits, with high degree crystallinity phases and diatom remnants, in addition to alunite + kaolinite + opal CT + anatase assemblages, is consistent with a superimposition of a steam-heated environment to a previous sinter deposit. These characters are also a distinguishing feature of paleosurface deposits associated with the geothermal system of the Cordón de Inacaliri Volcanic Complex. The presence of diatoms in heated freshwater bodies at 5100 m a.s.l. in the Atacama Desert environment could be related with the last documented deglaciation in the area (~20–10 ka), an important factor in the recharge of the hidden geothermal systems of the Pabelloncito graben.
Towards Understanding the Involvement of H+-ATPase in Programmed Cell Death of Psammosilene tunicoides after Oxalic Acid Application
Authors: Jiang Xinyu , Mohammadi Mohammad Aqa , Qin Yuan , Zhang Zongshen
Published: November 2021

Psammosilene tunicoides is a unique perennial medicinal plant species native to the Southwestern regions of China. Its wild population is rare and endangered due to over-excessive collection and extended growth (4–5 years). This research shows that H+-ATPase activity was a key factor for oxalate-inducing programmed cell death (PCD) of P. tunicoides suspension cells. Oxalic acid (OA) is an effective abiotic elicitor that enhances a plant cell’s resistance to environmental stress. However, the role of OA in this process remains to be mechanistically unveiled. The present study evaluated the role of OA-induced cell death using an inverted fluorescence microscope after staining with Evans blue, FDA, PI, and Rd123. OA-stimulated changes in K+ and Ca2+ trans-membrane flows using a patch-clamp method, together with OA modulation of H+-ATPase activity, were further examined. OA treatment increased cell death rate in a dosage-and duration-dependent manner. OA significantly decreased the mitochondria activity and damaged its electron transport chain. The OA treatment also decreased intracellular pH, while the FC increased the pH value. Simultaneously, NH4Cl caused intracellular acidification. The OA treatment independently resulted in 90% and the FC led to 25% cell death rates. Consistently, the combined treatments caused a 31% cell death rate. Furthermore, treatment with EGTA caused a similar change in intracellular pH value to the La3+ and OA application. Combined results suggest that OA-caused cell death could be attributed to intracellular acidification and the involvement of OA in the influx of extracellular Ca2+, thereby leading to membrane depolarization. Here we explore the resistance mechanism of P. tunicoides cells against various stresses endowed by OA treatment.
Evaluation of the Removal of Selected Phthalic Acid Esters (PAEs) in Municipal Wastewater Treatment Plants Supported by Constructed Wetlands
Authors: Wolecki Daniel , Trella Barbara , Qi Fei , Stepnowski Piotr , Kumirska Jolanta
Published: November 2021

Phthalic acid esters (PAEs) have a negative impact on living organisms in the environment, therefore, are among the group of Endocrine Disrupting Compounds (ECDs). Unfortunately, conventional methods used in municipal wastewater treatment plants (MWWTPs) are not designed to eliminate PAEs. For this reason, the development of cheap and simple but very effective techniques for the removal of such residues from wastewater is crucial. The main aim of this study was the evaluation of the removal of six selected PAEs: diethyl phthalate (DEP), di-n-octyl phthalate (DOP), di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), bis(2-ethylhexyl) phthalate (DEHP) and dimethyl phthalate (DMP), in real MWWTPs supported by constructed wetlands (MWWTP–CW system). For the first time, the possibility of using three new plants for this purpose, Cyperus papyrus (papyrus), Lysimachia nemorum (yellow pimpernel) and Euonymus europaeus (European spindle), has been presented. For determining the target PAEs in wastewater samples, a method of SPE (Solid-Phase Extraction)–GC–MS(SIM) was developed and validated, and for plant materials, a method of UAE (Ultrasound-Assisted Extraction)–SPE–GC–MS(SIM) was proposed. The obtained data showed that the application of the MWWTP–CW system allows a significant increase in the removal of DEP, DBP, BBP and DEHP from the wastewater stream. Euonymus europaeus was the most effective among the tested plant species for the uptake of analytes (8938 ng × g−1 dry weight), thus, this plant was found to be optimal for supporting conventional MWWTPs.
Red Quinoa Bran Extract Prevented Alcoholic Fatty Liver Disease via Increasing Antioxidative System and Repressing Fatty Acid Synthesis Factors in Mice Fed Alcohol Liquid Diet
Authors: Lin Ting-An , Ke Bo-Jun , Cheng Shih-Cheng , Lee Chun-Lin
Published: November 2021

Alcohol is metabolized in liver. Chronic alcohol abuse results in alcohol-induced fatty liver and liver injury. Red quinoa (Chenopodium formosanum) was a traditional staple food for Taiwanese aborigines. Red quinoa bran (RQB) included strong anti-oxidative and anti-inflammatory polyphenolic compounds, but it was usually regarded as the agricultural waste. Therefore, this study is to investigate the effect of water and ethanol extraction products of RQB on the prevention of liquid alcoholic diet-induced acute liver injury in mice. The mice were given whole grain powder of red quinoa (RQ-P), RQB ethanol extract (RQB-E), RQB water extract (RQB-W), and rutin orally for 6 weeks, respectively. The results indicated that RQB-E, RQB-W, and rutin decreased alcoholic diet-induced activities of aspartate aminotransferase and alanine aminotransferase, and the levels of serum triglyceride, total cholesterol, and hepatic triglyceride. Hematoxylin and eosin staining of liver tissues showed that RQB-E and RQB-W reduced lipid droplet accumulation and liver injury. However, ethanol extraction process can gain high rutin and antioxidative agents contents from red quinoa, that showed strong effects in preventing alcoholic fatty liver disease and liver injury via increasing superoxide dismutase/catalase antioxidative system and repressing the expressions of fatty acid synthesis enzyme acetyl-CoA carboxylase.
Effect of Laser Radiation on the Dynamics of Active Brownian Macroparticles in an Extended Plasma-Dust Monolayer
Authors: Fairushin Ilnaz Izailovich , Vasiliev Mikhail Mikhailovich , Petrov Oleg Fedorovich
Published: November 2021

Using the modified method of Brownian dynamics, the dynamics of macroparticles with a uniform metal coating in a plasma-dust monolayer under the action of laser radiation was simulated. The time dependences of the root-mean-square and average linear displacements of particles were calculated for different initial effective parameters of nonideality and different intensities of laser radiation. A relationship was established that connects the effective parameter of nonideality of the dusty plasma system of active particles with the maximum value of the mean linear displacement of particles.
Structural Effects of Disease-Related Mutations in Actin-Binding Period 3 of Tropomyosin
Authors: Kuruba Balaganesh , Kaczmarek Marta , Kęsik-Brodacka Małgorzata , Fojutowska Magdalena , Śliwinska Małgorzata , Kostyukova Alla S. , Moraczewska Joanna
Published: November 2021

Tropomyosin (Tpm) is an actin-binding coiled-coil protein. In muscle, it regulates contractions in a troponin/Ca2+-dependent manner and controls the thin filament lengths at the pointed end. Due to its size and periodic structure, it is difficult to observe small local structural changes in the coiled coil caused by disease-related mutations. In this study, we designed 97-residue peptides, Tpm1.164–154 and Tpm3.1265–155, focusing on the actin-binding period 3 of two muscle isoforms. Using these peptides, we evaluated the effects of cardiomyopathy mutations: I92T and V95A in Tpm1.1, and congenital myopathy mutations R91P and R91C in Tpm3.12. We introduced a cysteine at the N-terminus of each fragment to promote the formation of the coiled-coil structure by disulfide bonds. Dimerization of the designed peptides was confirmed by gel electrophoresis in the presence and absence of dithiothreitol. Using circular dichroism, we showed that all mutations decreased coiled coil stability, with Tpm3.1265–155R91P and Tpm1.164–154I92T having the most drastic effects. Our experiments also indicated that adding the N-terminal cysteine increased coiled coil stability demonstrating that our design can serve as an effective tool in studying the coiled-coil fragments of various proteins.
New Models to Predict the Acute and Chronic Toxicities of Representative Species of the Main Trophic Levels of Aquatic Environments
Authors: Toma Cosimo , Cappelli Claudia I. , Manganaro Alberto , Lombardo Anna , Arning Jürgen , Benfenati Emilio
Published: November 2021

To assess the impact of chemicals on an aquatic environment, toxicological data for three trophic levels are needed to address the chronic and acute toxicities. The use of non-testing methods, such as predictive computational models, was proposed to avoid or reduce the need for animal models and speed up the process when there are many substances to be tested. We developed predictive models for Raphidocelis subcapitata, Daphnia magna, and fish for acute and chronic toxicities. The random forest machine learning approach gave the best results. The models gave good statistical quality for all endpoints. These models are freely available for use as individual models in the VEGA platform and for prioritization in JANUS software.
GC/MS Analysis of Essential Oil and Enzyme Inhibitory Activities of Syzygium cumini (Pamposia) Grown in Egypt: Chemical Characterization and Molecular Docking Studies
Authors: El-Nashar Heba A. S. , Eldehna Wagdy M. , Al-Rashood Sara T. , Alharbi Amal , Eskandrani Razan O. , Aly Shaza H.
Published: November 2021

Syzygium cumini (Pomposia) is a well-known aromatic plant belonging to the family Myrtaceae, and has been reported for its various traditional and pharmacological potentials, such as its antioxidant, antimicrobial, anti-inflammatory, and antidiarrheal properties. The chemical composition of the leaf essential oil via gas chromatography–mass spectrometry (GC/MS) analysis revealed the identification of fifty-three compounds representing about 91.22% of the total oil. The identified oil was predominated by α-pinene (21.09%), followed by β-(E)-ocimene (11.80%), D-limonene (8.08%), β-pinene (7.33%), and α-terpineol (5.38%). The tested oil revealed a moderate cytotoxic effect against human liver cancer cells (HepG2) with an IC50 value of 38.15 ± 2.09 µg/mL. In addition, it effectively inhibited acetylcholinesterase with an IC50 value of 32.9 ± 2.1 µg/mL. Furthermore, it showed inhibitory properties against α-amylase and α-glucosidase with IC50 values of 57.80 ± 3.30 and 274.03 ± 12.37 µg/mL, respectively. The molecular docking studies revealed that (E)-β-caryophyllene, one of the major compounds, achieved the best docking scores of −6.75, −5.61, and −7.75 for acetylcholinesterase, α-amylase, and α-glucosidase, respectively. Thus, it is concluded that S. cumini oil should be considered as a food supplement for the elderly to enhance memory performance and for diabetic patients to control blood glucose.
Validation of the Developed Zero-Order Infrared Spectrophotometry Method for Qualitative and Quantitative Analyses of Tranexamic Acid in Marketed Tablets
Authors: Nerdy Nerdy , Margata Linda , Sembiring Bunga Mari , Ginting Selamat , Putra Effendy De Lux , Bakri Tedy Kurniawan
Published: November 2021

(1) Background: The functional groups present in tranexamic acid allow direct infrared detection analysis. This study aimed to develop, apply, and validate an infrared spectrophotometry method used for qualitative and quantitative analyses of tranexamic acid in marketed tablets. (2) Methods: This was a descriptive observational study that consisted of several stages: determining the specific wavenumber for analysis, obtaining a simple linear regression equation, analyzing tranexamic acid both qualitatively and quantitatively, and validating the developed method for routine analysis. (3) Results: The peak analysis obtained a range of baseline wavenumbers from 1679.17 to 1295.25 cm−1. The regression equation obtained was Y = 310.8527 × X + 0.9718, and the coefficient of determination (R2) obtained was 0.9994. The tranexamic acids in marketed tablets overall have a similarity index value of more than 0.90 and overall have levels ranging between 97.0% and 103.0%. The infrared spectrophotometry method that was successfully developed, applied, and validated for qualitative and quantitative analyses of tranexamic acid in marketed tablets meets the requirements both qualitatively and quantitatively of the tablet monograph. (4) Conclusions: The infrared spectrophotometry method has been validated and meets the requirements for accuracy, precision, detection limit, quantitation limit, linearity, range, and specificity.
Preparation of Ag3PO4/TiO2(B) Heterojunction Nanobelt with Extended Light Response and Enhanced Photocatalytic Performance
Authors: Li Yong , Liu Yanfang , Zhang Mingqing , Zhou Qianyu , Li Xin , Chen Tianlu , Wang Shifeng
Published: November 2021

Photocatalytic degradation, as an emerging method to control environmental pollution, is considered one of the most promising environmental purification technologies. As Tibet is a region with some of the strongest solar radiation in China and even in the world, it is extremely rich in solar energy resources, which is ideal for applying photocatalytic technology to its ecological environment protection and governance. In this study, Na2Ti3O7 nanobelts were prepared via a hydrothermal method and converted to TiO2∙xH2O ion exchange, which was followed by high-temperature calcination to prepare TiO2(B) nanobelts (“B” in TiO2(B) means “Bronze phase”). A simple in situ method was used to generate Ag3PO4 particles on the surface of the TiO2 nanobelts to construct a Ag3PO4/TiO2(B) heterojunction composite photocatalyst. By generating Ag3PO4 nanoparticles on the surface of the TiO2(B) nanobelts to construct heterojunctions, the light absorption range of the photocatalyst was successfully extended from UV (ultraviolet) to the visible region. Furthermore, the recombination of photogenerated electron–hole pairs in the catalyst was inhibited by the construction of the heterojunctions, thus greatly enhancing its light quantum efficiency. Therefore, the prepared Ag3PO4/TiO2(B) heterojunction composite photocatalyst greatly outperformed the TiO2(B) nanobelt in terms of photocatalytic degradation.
Identification and Quantification of Hydrocarbon Functional Groups in Gasoline Using 1H-NMR Spectroscopy for Property Prediction
Authors: Abdul Jameel Abdul Gani
Published: November 2021

Gasoline is one of the most important distillate fuels obtained from crude refining; it is mainly used as an automotive fuel to propel spark-ignited (SI) engines. It is a complex hydrocarbon fuel that is known to possess several hundred individual molecules of varying sizes and chemical classes. These large numbers of individual molecules can be assembled into a finite set of molecular moieties or functional groups that can independently represent the chemical composition. Identification and quantification of groups enables the prediction of many fuel properties that otherwise may be difficult and expensive to measure experimentally. In the present work, high resolution 1H nuclear magnetic resonance (NMR) spectroscopy, an advanced structure elucidation technique, was employed for the molecular characterization of a gasoline sample in order to analyze the functional groups. The chemical composition of the gasoline sample was then expressed using six hydrocarbon functional groups, as follows: paraffinic groups (CH, CH2 and CH3), naphthenic CH-CH2 groups and aromatic C-CH groups. The obtained functional groups were then used to predict a number of fuel properties, including research octane number (RON), motor octane number (MON), derived cetane number (DCN), threshold sooting index (TSI) and yield sooting index (YSI).
In Vivo Investigation of the Ameliorating Effect of Tempol against MIA-Induced Knee Osteoarthritis in Rats: Involvement of TGF-β1/SMAD3/NOX4 Cue
Authors: Abo-zalam Hagar B. , Abdelsalam Rania M. , Abdel-Rahman Rehab F. , Abd-Ellah Mohamed F. , Khattab Mahmoud M.
Published: November 2021

Osteoarthritis (OA) is a complex disease characterized by structural, functional, and metabolic deteriorations of the whole joint and periarticular tissues. In the current study, we aimed to investigate the possible effects of tempol on knee OA induced by the chemical chondrotoxic monosodium iodoacetate (MIA) which closely mimics both the pain and structural changes associated with human OA. Rats were administrated oral tempol (100 mg/kg) one week post-MIA injection (3 mg/50 μL saline) at the right knee joints for 21 consecutive days. Tempol improved motor performance and debilitated the MIA-related radiological and histological alterations. Moreover, it subsided the knee joint swelling. Tempol decreased the cartilage degradation-related biomarkers as matrix metalloproteinase-13, bone alkaline phosphatase (bone ALP), and fibulin-3. The superoxide dismutase mimetic effect of tempol was accompanied by decreased NADPH oxidase 4 (NOX4), inflammatory mediators, nuclear factor-kappa B (NF-κB), over-released transforming growth factor-β1 (TGF-β1). Tempol decreased the expression of chemokine (C-C motif) ligand 2 (CCL2). On the molecular level, tempol reduced the phosphorylated protein levels of p38 mitogen-activated protein kinase (MAPK), and small mother against decapentaplegic 3 homologs (SMAD3). These findings suggest the promising role of tempol in ameliorating MIA-induced knee OA in rats via collateral suppression of the catabolic signaling cascades including TGF-β1/SMAD3/NOX4, and NOX4/p38MAPK/NF-κB and therefore modulation of oxidative stress, catabolic inflammatory cascades, chondrocyte metabolic homeostasis.
CdIn2S4/In(OH)3/NiCr-LDH Multi-Interface Heterostructure Photocatalyst for Enhanced Photocatalytic H2 Evolution and Cr(VI) Reduction
Authors: Fu Rao , Gong Yinyan , Li Can , Niu Lengyuan , Liu Xinjuan
Published: November 2021

The development of highly active and stable photocatalysts, an effective way to remediate environment pollution and alleviate energy shortages, remains a challenging issue. In this work, a CdIn2S4/In(OH)3 nanocomposite was deposited in-situ on NiCr-LDH nanosheets by a simple hydrothermal method, and the obtained CdIn2S4/In(OH)3/NiCr-LDH heterostructure photocatalysts with multiple intimate-contact interfaces exhibited better photocatalytic activity. The photocatalytic H2 evolution rate of CdIn2S4/In(OH)3/NiCr-LDH increased to 10.9 and 58.7 times that of the counterparts CdIn2S4 and NiCr-LDH, respectively. Moreover, the photocatalytic removal efficiency of Cr(VI) increased from 6% for NiCr-LDH and 75% for CdIn2S4 to 97% for CdIn2S4/In(OH)3/NiCr-LDH. The enhanced photocatalytic performance was attributed to the formation of multi-interfaces with strong interfacial interactions and staggered band alignments, which offered multiple pathways for carrier migration, thus promoting the separation efficiency of photo-excited electrons and holes. This study demonstrates a facile method to fabricate inexpensive and efficient heterostructure photocatalysts for solving environmental problems.
Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of Rhodococcus erythropolis IGTS8
Authors: Prasoulas George , Dimos Konstantinos , Glekas Panayiotis , Kalantzi Styliani , Sarris Stamatis , Templis Chrysovalantis , Vavitsas Konstantinos , Hatzinikolaou Dimitris G. , Papayannakos Nikolaos , Kekos Dimitris , Mamma Diomi
Published: November 2021

Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for the removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in a bubble column bioreactor using two-phase media. The effects of various process parameters, such as biocatalyst age and concentration, organic fraction percentage (OFP), and type of sulfur compound—namely, dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT), and 4,6-diethyldibenzothiophene (4,6-DEDBT)—were evaluated, using resting cells of Rhodococcus erythropolis IGTS8. Cells derived from the beginning of the exponential growth phase of the bacterium exhibited the highest biodesulfurization efficiency and rate. The biocatalyst performed better in an OFP of 50% v/v. The extent of DBT desulfurization was dependent on cell concentration, with the desulfurization rate reaching its maximum at intermediate cell concentrations. A new semi-empirical model for the biphasic BDS was developed, based on the overall Michaelis-Menten kinetics and taking into consideration the deactivation of the biocatalyst over time, as well as the underlying mass transfer phenomena. The model fitted experimental data on DBT consumption and 2-hydroxibyphenyl (2-HBP) accumulation in the organic phase for various initial DBT concentrations and different organosulfur compounds. For constant OFP and biocatalyst concentration, the most important parameter that affects BDS efficiency seems to be biocatalyst deactivation, while the phenomenon is controlled by the affinities of biodesulfurizing enzymes for the different organosulfur compounds. Thus, desulfurization efficiency decreased with increasing initial DBT concentration, and in inverse proportion to increases in the carbon number of alkyl substituent groups.
The Effect of PVA Binder Solvent Composition on the Microstructure and Electrical Properties of 0.98BaTiO3-0.02(Ba0.5Ca0.5)SiO3 Doped with Dy2O3
Authors: Jo Nak-Beom , Baek Jin-Seok , Kim Eung-Soo
Published: November 2021

In this study, the effect of the polyvinyl alcohol (PVA) binder solvent composition on the electrical properties of sintered 0.98BaTiO3-0.02(Ba0.5Ca0.5)SiO3 ceramics doped with x wt.% Dy2O3 (0.0 ≤ x ≤ 0.3) was investigated. In the absence of the PVA binder, the specimens sintered at 1260 and 1320 °C for 1 h in a reducing atmosphere showed a single BaTiO3 phase with the perovskite structure. The relative densities of the specimens were higher than 90%, and the grain morphologies were uniform for all the solvent compositions. At 1 kHz, the dielectric constant of the specimens depended not only on their crystal structural characteristics, but also on their microstructural characteristics. The microstructural characteristics of the specimens with the PVA binder were affected by the ethyl alcohol:water ratio of the 10 wt.% PVA-111 solution. A homogeneous microstructure was observed for the 0.1 wt.% Dy2O3-doped specimens sintered at 1320 °C for 1 h when the ethyl alcohol/water ratio of the binder solution was 40/60. These specimens showed the maximum dielectric constant (εr = 2723.3) and an insulation resistance of 270 GΩ. The relationships between the microstructural characteristics and dissipation factor (tanδ) of the specimens were also investigated.