bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Temperature Exposition of Casting Solution on Properties of Polysulfone Hollow Fiber Membranes
Authors: Borisov Ilya , Vasilevsky Vladimir , Matveev Dmitry , Ovcharova Anna , Volkov Alexey , Volkov Vladimir
Published: December 2019

It was shown for the first time that the conditions of thermal treatment of the casting solution significantly affect the morphology and transport properties of porous, flat, and hollow fiber polysulfone (PSf) membranes. It is ascertained that the main solution components that are subjected to thermo-oxidative destruction are the pore-forming agent polyethylene glycol (PEG) and solvent N-methyl-2-pyrrolidone (NMP). It is proved that hydroxyl groups of PEG actively react in the process of the casting solution thermo-oxidative destruction. It is shown that despite the chemical conversion taking place in the casting solution, their stability towards coagulation virtually does not change. The differences in the membrane morphology associated with the increase of thermal treatment time at 120 °C are not connected to the thermodynamic properties of the casting solutions, but with the kinetics of the phase separation. It is revealed that the change of morphology and transport properties of membranes is connected with the increase of the casting solution viscosity. The rise of solution viscosity resulted in the slowdown of the phase separation and formation of a more densely packed membrane structure with less pronounced macropores. It is determined experimentally that with the increase of casting solution thermal treatment time, the membrane selective layer thickness increases. This leads to the decrease of gas permeance and the rise of the He/CO2 selectivity for flat and hollow fiber membranes. In the case of hollow fibers, the fall of gas permeance is also connected with the appearance of the sponge-like layer at the outer surface of membranes. The increase of selectivity and decline of permeance indicates the reduction of selective layer pore size and its densification, which agrees well with the calculation results of the average membrane density. The results obtained are relevant to any polymeric casting solution containing NMP and/or PEG and treated at temperatures above 60 °C.
Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses
Authors: Yan Lianlian , Xu Ruiping , Bian Yinbing , Li Hongxian , Zhou Yan
Published: December 2019

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.
Comparison of the JNC7 and 2017 American College of Cardiology/American Heart Association Guidelines for the Management of Hypertension in Koreans: Analysis of Two Independent Nationwide Population-Based Studies
Authors: Choi Won-Jun , Lee Hye-Sun , Hong Jung Hwa , Chang Hyuk-Jae , Lee Ji-Won
Published: December 2019

The optimal blood pressure (BP) guidelines in Asian populations have not been determined. We compared all-cause and cardiovascular mortality based on the Joint National Committee 7 (JNC7) and 2017 American College of Cardiology/American Heart Association (ACC/AHA) guidelines. The National Health Insurance System-National Health Screening Cohort (NHIS-HEALS) and Korea National Health and Nutrition Examination Survey (KNHANES) were utilized. BPs were classified into three groups according to each guideline, and survival rates were analyzed with Kaplan-Meier curves and log-rank tests. Hazard ratios (HRs) were calculated using multivariable cox regression analyses, and the discriminatory ability for clinical outcomes was assessed by Harrell’s C-indexes. The JNC7 guidelines demonstrated a linear association between BP levels and survival outcomes. Adjusted HRs from the JNC7 guidelines differentiated the hypertension group (≥140/90) from the pre (130/80–139/89) and normal (<130 and <80) BP groups in clinical outcomes. In contrast, the 2017 ACC/AHA guidelines showed inconsistent survival outcomes according to BP classification (normal: <120 and <80, elevated: 120–129, and <80, and HTN: ≥130/80). According to Harrell’s C-indexes, the JNC7 guidelines had greater discrimination ability in survival outcomes in the NHIS-HEALS dataset. Our results suggest that the JNC7 guidelines are more appropriate than the 2017 ACC/AHA guidelines in Korean populations.
Comparability of ActivPAL-Based Estimates of Meeting Physical Activity Guidelines for Preschool Children
Authors: Huang Wendy Yajun , Lee Eun-Young
Published: December 2019

The activPAL (PAL Technologies, Glasgow, UK) has been increasingly used on children to assess sedentary time and physical activity (PA). However, there is no consensus on how it can estimate PA at different intensities. This study compared three commonly used, activPAL-based classifications of moderate-to-vigorous physical activity (MVPA) (daily steps, acceleration counts, and step rate) in determining compliance with the World Health Organization (WHO)’s PA guidelines for preschool children on a daily basis. One hundred and fourteen preschool children aged 3–6 years wore an activPALTM for 24 h over 7 consecutive days and provided valid data for a total of 548 days. MVPA was calculated based on published cut-points of counts (MVPA-counts) and step rate (MVPA-step rate). Compliance with standard PA guidelines (≥180 min/day of PA including ≥60 min/day of MVPA) was determined based on three criteria: ≥11,500 steps/day, a threshold of 1418 acceleration counts/15 s, and 25 steps/15 s for MVPA. Applying cut-points of daily steps and acceleration counts provided the same estimates of compliance with the WHO PA guidelines (20%), while the estimated compliance based on the step rate was lower (7.7%). There was a moderate agreement between the daily steps- (or counts-) derived and step rate-derived compliances (κ = 0.41; 95% confidence interval (CI): 0.31, 0.51). The amount of MVPA derived from counts (1.95 ± 0.72 h/day) was significantly higher than that from step rates (0.47 ± 0.31 h/day). The activPAL may be useful for surveillance studies to estimate total PA in preschool children. Further development of the activPAL algorithms based on either counts or step rate is warranted before it can be used to accurately estimate MVPA in this age group.
From Egoism to Ecoism: Psychedelics Increase Nature Relatedness in a State-Mediated and Context-Dependent Manner
Authors: Kettner Hannes , Gandy Sam , Haijen Eline C. H. M. , Carhart-Harris Robin L.
Published: December 2019

(1) Background: There appears to be a growing disconnection between humans and their natural environments which has been linked to poor mental health and ecological destruction. Previous research suggests that individual levels of nature relatedness can be increased through the use of classical psychedelic compounds, although a causal link between psychedelic use and nature relatedness has not yet been established. (2) Methods: Using correlations and generalized linear mixed regression modelling, we investigated the association between psychedelic use and nature relatedness in a prospective online study. Individuals planning to use a psychedelic received questionnaires 1 week before (N = 654), plus one day, 2 weeks, 4 weeks, and 2 years after a psychedelic experience. (3) Results: The frequency of lifetime psychedelic use was positively correlated with nature relatedness at baseline. Nature relatedness was significantly increased 2 weeks, 4 weeks and 2 years after the psychedelic experience. This increase was positively correlated with concomitant increases in psychological well-being and was dependent on the extent of ego-dissolution and the perceived influence of natural surroundings during the acute psychedelic state. (4) Conclusions: The here presented evidence for a context- and state-dependent causal effect of psychedelic use on nature relatedness bears relevance for psychedelic treatment models in mental health and, in the face of the current ecological crisis, planetary health.
Humanized Mice as an Effective Evaluation System for Peptide Vaccines and Immune Checkpoint Inhibitors
Authors: Kametani Yoshie , Ohno Yusuke , Ohshima Shino , Tsuda Banri , Yasuda Atsushi , Seki Toshiro , Ito Ryoji , Tokuda Yutaka
Published: December 2019

Peptide vaccination was developed for the prevention and therapy of acute and chronic infectious diseases and cancer. However, vaccine development is challenging, because the patient immune system requires the appropriate human leukocyte antigen (HLA) recognition with the peptide. Moreover, antigens sometimes induce a low response, even if the peptide is presented by antigen-presenting cells and T cells recognize it. This is because the patient immunity is dampened or restricted by environmental factors. Even if the immune system responds appropriately, newly-developed immune checkpoint inhibitors (ICIs), which are used to increase the immune response against cancer, make the immune environment more complex. The ICIs may activate T cells, although the ratio of responsive patients is not high. However, the vaccine may induce some immune adverse effects in the presence of ICIs. Therefore, a system is needed to predict such risks. Humanized mouse systems possessing human immune cells have been developed to examine human immunity in vivo. One of the systems which uses transplanted human peripheral blood mononuclear cells (PBMCs) may become a new diagnosis strategy. Various humanized mouse systems are being developed and will become good tools for the prediction of antibody response and immune adverse effects.
Knockout of the S-acyltransferase Gene, PbPAT14, Confers the Dwarf Yellowing Phenotype in First Generation Pear by ABA Accumulation
Authors: Pang Hongguang , Yan Qi , Zhao Shuliang , He Fang , Xu Jianfeng , Qi Baoxiu , Zhang Yuxing
Published: December 2019

The development of dwarf fruit trees with smaller and compact characteristics leads to significantly increased fruit production, which is a major objective of pear (Pyrus bretschneideri) breeding. We identified the S-acylation activity of PbPAT14, an S-acyltransferase gene related to plant development, using a yeast (Saccharomyces cerevisiae) complementation assay, and also PbPAT14 could rescue the growth defect of the Arabidopsis mutant atpat14. We further studied the function of PbPAT14 by designing three guide RNAs for PbPAT14 to use in the CRISPR/Cas9 system. We obtained 22 positive transgenic pear lines via Agrobacterium-mediated transformation using cotyledons from seeds of Pyrus betulifolia (‘Duli’). Six of these lines exhibited the dwarf yellowing phenotype and were homozygous mutations according to sequencing analysis. Ultrastructure analysis suggested that this dwarfism was manifested by shorter, thinner stems due to a reduction in cell number. A higher level of endogenous abscisic acid (ABA) and a higher transcript level of the ABA pathway genes in the mutant lines revealed that the PbPAT14 function was related to the ABA pathway. Overall, our experimental results increase the understanding of how PATs function in plants and help elucidate the mechanism of plant dwarfism.
Sequential Transcriptome Changes in the Penumbra after Ischemic Stroke
Authors: Choi In-Ae , Yun Ji Hee , Kim Ji-Hye , Kim Hahn Young , Choi Dong-Hee , Lee Jongmin
Published: December 2019

To investigate the changes in the expression of specific genes that occur during the acute-to-chronic post-stroke phase, we identified differentially expressed genes (DEGs) between naive cortical tissues and peri-infarct tissues at 1, 4, and 8 weeks after photothrombotic stroke. The profiles of DEGs were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology analyses, followed by string analysis of the protein–protein interactions (PPI) of the products of these genes. We found 3771, 536, and 533 DEGs at 1, 4, and 8 weeks after stroke, respectively. A marked decrease in biological–process categories, such as brain development and memory, and a decrease in neurotransmitter synaptic and signaling pathways were observed 1 week after stroke. The PPI analysis showed the downregulation of Dlg4, Bdnf, Gria1, Rhoa, Mapk8, and glutamatergic receptors. An increase in biological–process categories, including cell population proliferation, cell adhesion, and inflammatory responses, was detected at 4 and 8 weeks post-stroke. The KEGG pathways of complement and coagulation cascades, phagosomes, antigen processing, and antigen presentation were also altered. CD44, C1, Fcgr2b, Spp1, and Cd74 occupied a prominent position in network analyses. These time-dependent changes in gene profiles reveal the unique pathophysiological characteristics of stroke and suggest new therapeutic targets for this disease.
A Machine-Learning Model for Zonal Ship Flow Prediction Using AIS Data: A Case Study in the South Atlantic States Region
Authors: Wang Xuantong , Li Jing , Zhang Tong
Published: December 2019

Predicting traffic flow is critical in efficient maritime transportation management, coordination, and planning. Scientists have proposed many prediction methods, most of which are designed for specific locations or for short-term prediction. For the purpose of management, methods that enable long-term prediction for large areas are highly desirable. Therefore, we propose developing a spatiotemporal approach that can describe and predict traffic flows within a region. We designed the model based on a multiple hexagon-based convolutional neural network (mh-CNN) model that takes both the flow dynamics and environmental conditions into account. This model is highly flexible in that it predicts zonal traffic flow within variable time windows. We applied the method to measure and predict the daily and hourly traffic flows in the South Atlantic States region by taking the impacts of extreme weather events into consideration. Results show that our method outperformed other methods in daily prediction during normal days and hourly prediction during hurricane events. Based on the results, we also provide some recommendations regarding the future usage and customization of the model.
Immune Parameters for Diagnosis and Treatment Monitoring in Invasive Mold Infection
Authors: Jenks Jeffrey , Rawlings Stephen , Garcia-Vidal Carol , Koehler Philipp , Mercier Toine , Prattes Juergen , Lass-Flörl Cornelia , Martin-Gomez M , Buchheidt Dieter , Pagano Livio , Gangneux Jean-Pierre , van de Veerdonk Frank , Netea Mihai , Carvalho Agostinho , Hoenigl Martin
Published: December 2019

Infections caused by invasive molds, including Aspergillus spp., can be difficult to diagnose and remain associated with high morbidity and mortality. Thus, early diagnosis and targeted systemic antifungal treatment remains the most important predictive factor for a successful outcome in immunocompromised individuals with invasive mold infections. Diagnosis remains difficult due to low sensitivities of diagnostic tests including culture and other mycological tests for mold pathogens, particularly in patients on mold-active antifungal prophylaxis. As a result, antifungal treatment is rarely targeted and reliable markers for treatment monitoring and outcome prediction are missing. Thus, there is a need for improved markers to diagnose invasive mold infections, monitor response to treatment, and assist in determining when antifungal therapy should be escalated, switched, or can be stopped. This review focuses on the role of immunologic markers and specifically cytokines in diagnosis and treatment monitoring of invasive mold infections.
The AC Soft Magnetic Properties of FeCoNixCuAl (1.0 ≤ x ≤ 1.75) High-Entropy Alloys
Authors: Wu Zhongyuan , Wang Chenxu , Zhang Yin , Feng Xiaomeng , Gu Yong , Li Zhong , Jiao Huisheng , Tan Xiaohua , Xu Hui
Published: December 2019

High-entropy alloys (HEAs) with soft magnetic properties are one of the new candidate soft magnetic materials which are usually used under an alternating current (AC) magnetic field. In this work, the AC soft magnetic properties are investigated for FeCoNixCuAl (1.0 ≤ x ≤ 1.75) HEAs. The X-ray diffraction (XRD) and scanning electron microscope (SEM) show that the alloy consists of two phases, namely a face-centred cubic (FCC) phase and a body-centred cubic (BCC) phase. With increasing Ni content, the FCC phase content increased. Further research shows that the AC soft magnetic properties of these alloys are closely related to their phase constitution. Increasing the FCC phase content contributes to a decrease in the values of AC remanence (AC Br), AC coercivity (AC Hc) and AC total loss (Ps), while it is harmful to the AC maximum magnetic flux density (AC Bm). Ps can be divided into two parts: AC hysteresis loss (Ph) and eddy current loss (Pe). With increasing frequency f, the ratio of Ph/Ps decreases for all samples. When f ≤ 150 Hz, Ph/Ps > 70%, which means that Ph mainly contributes to Ps. When f ≥ 800 Hz, Ph/Ps < 40% (except for the x = 1.0 sample), which means that Pe mainly contributes to Ps. At the same frequency, the ratio of Ph/Ps decreases gradually with increasing FCC phase content. The values of Pe and Ph are mainly related to the electrical resistivity (ρ) and the AC Hc, respectively. This provides a direction to reduce Ps.
Improving the Tribological Performance of MAO Coatings by Using a Stable Sol Electrolyte Mixed with Cellulose Additive
Authors: Song Wei , Jiang Bailing , Ji Dongdong
Published: December 2019

In this study, micro-arc oxidation (MAO) of aluminum 6061 alloy was carried out within a silicate base electrolyte containing 0.75 g/L of cellulose, and the tribological properties of the coating were investigated. The as-prepared coating was detected by Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), a scanning electron microscope (SEM) and an energy-dispersive spectrometer (EDS), respectively. The results suggested that cellulose filled in the microcracks and micropores, or it existed by cross-linking with Al3+. In addition, it was found that the cellulose had little effect on the coating hardness. However, the thickness and roughness of the coating were improved with the increase in cellulose concentration. Moreover, the ball-on-disk test showed that the friction coefficient, weight loss and wear rate of the MAO coating decreased with the increase in cellulose concentration. Further, the performances of the coatings obtained in the same electrolyte, under different preserved storage periods, were compared, revealing that the cellulose was uniformly dispersed in the electrolyte and improved the tribological properties of the MAO coating within 30 days.
Extraction of Metal Ions with Metal–Organic Frameworks
Authors: Manousi Natalia , Giannakoudakis Dimitrios A. , Rosenberg Erwin , Zachariadis George A.
Published: December 2019

Metal–organic frameworks (MOFs) are crystalline porous materials composed of metal ions or clusters coordinated with organic linkers. Due to their extraordinary properties such as high porosity with homogeneous and tunable in size pores/cages, as well as high thermal and chemical stability, MOFs have gained attention in diverse analytical applications. MOFs have been coupled with a wide variety of extraction techniques including solid-phase extraction (SPE), dispersive solid-phase extraction (d-SPE), and magnetic solid-phase extraction (MSPE) for the extraction and preconcentration of metal ions from complex matrices. The low concentration levels of metal ions in real samples including food samples, environmental samples, and biological samples, as well as the increased number of potentially interfering ions, make the determination of trace levels of metal ions still challenging. A wide variety of MOF materials have been employed for the extraction of metals from sample matrices prior to their determination with spectrometric techniques.
Correction: Kumar, M.R., et al. Characterization of Polysulfides, Polysulfanes, and Other Unique Species in the Reaction between GSNO and H2S. Molecules 2019, 24, 3090
Authors: Kumar Murugaeson R , Farmer Patrick J
Published: December 2019

The authors wish to make the following corrections to this paper [...]
Facile Synthesis of Triphenylamine Based Hyperbranched Polymer for Organic Field Effect Transistors
Authors: Bathula Chinna , Appiagyei Alfred , Yadav Hemraj , K. Ashok , Ramesh Sivalingam , Shrestha Nabeen , Shinde Surendra , Kim Hyun-Seok , Kim Heung , Reddy Lebaka , Mohammed Arifullah
Published: December 2019

In this study, we reported the synthesis and characterization of a novel hyperbranched polymer (HBPs) tris[(4-phenyl)amino-alt-4,8-bis(5-(2-ethylhexyl)thiophen-2-yl)benzo[1,2-b;4,5-b’]dithiophene] (PTPABDT) composed of benzo[1,2-b:4,5-b’]dithiophene (BDT) and triphenyleamine (TPA) constituent subunits by A3 + B2 type Stille’s reaction. An estimated optical band gap of 1.69 eV with HOMO and LUMO levels of −5.29 eV and −3.60 eV, respectively, as well as a high thermal stability up to 398 °C were characterized for the synthesized polymer. PTPABDT fabricated as an encapsulated top gate/bottom contact (TGBC), organic field effect transistors (OFET) exhibited a p-type behavior with maximum field-effect mobility (µmax) and an on/off ratio of 1.22 × 10−3 cm2 V−1 s−1 and 7.47 × 102, respectively.
Prioritizing the Scale-Up of Evidence-Based Nutrition and Health Interventions to Accelerate Stunting Reduction in Ethiopia
Authors: Baye Kaleab
Published: December 2019

Despite some progress, stunting prevalence in many African countries including Ethiopia remains unacceptably high. This study aimed to identify key interventions that, if implemented at scale through the health sector in Ethiopia, can avert the highest number of stunting cases. Using the Lives Saved Tool (LiST), the number of stunting cases that would have been averted, if proven interventions were scaled-up to the highest wealth quintile or to an aspirational 90% coverage was considered. Stunting prevalence was highest among rural residents and households in the poorest wealth quintile. Coverage of breastfeeding promotion and vitamin A supplementation were relatively high (>50%), whereas interventions targeting women were limited in number and had particularly low coverage. Universal coverage (90%) of optimal complementary feeding, preventive zinc supplementation, and water connection in homes could have each averted 380,000–500,000 cases of stunting. Increasing coverage of water connection to homes to the level of the wealthiest quintile could have averted an estimated 168,000 cases of stunting. Increasing coverage of optimal complementary feeding, preventive zinc supplementation, and Water, Sanitation and Hygiene (WASH) services is critical. Innovations in program delivery and health systems governance are required to effectively reach women, remote areas, rural communities, and the poorest proportion of the population to accelerate stunting reduction.
Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in C57bl/6 Models
Authors: Recena Aydos Leonardo , Aparecida do Amaral Luane , Serafim de Souza Roberta , Jacobowski Ana Cristina , Freitas dos Santos Elisvânia , Rodrigues Macedo Maria Lígia
Published: December 2019

Researchers have a range of animal models in which to study Nonalcoholic fatty liver disease (NAFLD). Induction of NAFLD by a high-fat diet in the C57BL/6 strain is the most widely used among mice. In this study, we review works that performed NAFLD induction by a high-fat diet using the C57BL/6 strain, focusing on experiments on the effects of lipid ingestion. Studies are initially distinguished into researches in which mice received lipids by oral gavage and studies in which lipid was added to the diet, and each of these designs has peculiarities that must be considered. Oral gavage can be stressful for animals and needs trained handlers but allows accurate control of the dose administered. The addition of oils to the diet can prevent stress caused to mice by gavage, but possible changes in the consistency, taste, and smell of the diet should be considered. Regarding the experimental design, some variables, such as animal sex, treatment time, and diet-related variables, appear to have a definite pattern. However, no pattern was found regarding the number of animals per group, age at the beginning of the experiment, time of adaptation, the substance used as a vehicle, and substance used as a control.
Mechanisms of Calorie Restriction: A Review of Genes Required for the Life-Extending and Tumor-Inhibiting Effects of Calorie Restriction
Authors: Komatsu Toshimitsu , Park Seongjoon , Hayashi Hiroko , Mori Ryoichi , Yamaza Haruyoshi , Shimokawa Isao
Published: December 2019

This review focuses on mechanisms of calorie restriction (CR), particularly the growth hormone (GH)/insulin-like growth factor-1 (IGF-1) axis as an evolutionary conserved signal that regulates aging and lifespan, underlying the effects of CR in mammals. Topics include (1) the relation of the GH-IGF-1 signal with chronic low-level inflammation as one of the possible causative factors of aging, that is, inflammaging, (2) the isoform specificity of the forkhead box protein O (FoxO) transcription factors in CR-mediated regulation of cancer and lifespan, (3) the role for FoxO1 in the tumor-inhibiting effect of CR, (4) pleiotropic roles for FoxO1 in the regulation of disorders, and (5) sirtuin (Sirt) as a molecule upstream of FoxO. From the evolutionary view, the necessity of neuropeptide Y (Npy) for the effects of CR and the pleiotropic roles for Npy in life stages are also emphasized. Genes for mediating the effects of CR and regulating aging are context-dependent, particularly depending on nutritional states.
Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress
Authors: Varghese Nisha , Alyammahi Onoud , Nasreddine Sarah , Alhassani Abla , Gururani Mayank Anand
Published: December 2019

Recent studies have demonstrated melatonin protects various crops against abiotic stresses. However, the effects of melatonin on the photosynthetic apparatus of stressed plants is poorly characterized. We investigated the effects of melatonin pretreatment on photosynthesis and tolerance to salinity stress in Avena sativa (oat) plants. Oat plants were exposed to four treatments (three replicate pots per treatment): well-watered (WW; control); watered with 300 mM salt solution for 10 days (NaCl); pretreated with 100 µM melatonin solution for 7 days then watered normally for 10 days (Mel+W); or pretreated with 100 µM melatonin for 7 days then 300 mM salt for 10 days (Mel+NaCl). Considerable differences in growth parameters, chlorophyll content, stomatal conductance, proline accumulation, lipid peroxidation, electrolyte leakage, and growth parameters were observed between groups. Genes encoding three major antioxidant enzymes were upregulated in the Mel+NaCl group compared to the other groups. Chlorophyll-a fluorescence kinetic analyses revealed that almost all photosynthetic parameters were improved in Mel+NaCl plants compared to the other treatments. Analysis of genes encoding the major extrinsic proteins of photosystem II (PSII) revealed that PsbA, PsbB, PsbC, and PsbD (but not PsbO) were highly upregulated in Mel+NaCl plants compared to the other groups, indicating melatonin positively influenced photosynthesis under control conditions and salt stress. In addition, melatonin upregulated stress-responsive NAC transcription factor genes in plants exposed to salt stress. These findings suggest melatonin pretreatment improves photosynthesis and enhances salt tolerance in oat plants.
Poly(Glycerol Sebacate)–Poly(l-Lactide) Nonwovens. Towards Attractive Electrospun Material for Tissue Engineering
Authors: Denis Piotr , Wrzecionek Michał , Gadomska-Gajadhur Agnieszka , Sajkiewicz Paweł
Published: December 2019

Two types of poly(glycerol sebacate) (PGS) prepolymers were synthesized and electrospun with poly(l-lactic acid) (PLA), resulting in bicomponent nonwovens. The obtained materials were pre-heated in a vacuum, at different times, to crosslink PGS and investigate morphological and structural dependencies in that polymeric, electrospun system. As both PGS and PLA are sensitive to pre-heating (crosslinking) conditions, research concerns both components. More interest is focused on the properties of PGS, considering further research for mechanical properties and subsequent experiments with PGS synthesis. Electrospinning of PGS blended with PLA does not bring difficulties, but obtaining elastomeric properties of nonwovens is problematic. Even though PGS has many potential advantages over other polyesters when soft tissue engineering is considered, its full utilization via the electrospinning process is much harder in practice. Further investigations are ongoing, especially with the promising PGS prepolymer with a higher esterification degree and its variations.
Experimental, Numerical, and Analytical Study on The Effect of Graphene Oxide in The Mechanical Properties of a Solvent-Free Reinforced Epoxy Resin
Authors: Horta Muñoz Sergio , Serna Moreno María del Carmen , González-Domínguez José Miguel , Morales-Rodríguez Pablo Antonio , Vázquez Ester
Published: December 2019

This paper presents a methodology for manufacturing nanocomposites from an epoxy resin reinforced with graphene oxide (GO) nanoparticles. A scalable and sustainable fabrication process, based on a solvent-free method, is proposed with the objective of achieving a high level of GO dispersion, while maintaining matrix performance. The results of three-point bending tests are examined by means of an analytical technique which allows determining the mechanical response of the material under tension and compression from flexural data. As result, an increase of 39% in the compressive elastic modulus of the nanocomposite is found with the addition of 0.3 wt % GO. In parallel, we described how the strain distribution and the failure modes vary with the amount of reinforcement based on digital image correlation (DIC) techniques and scanning electron microscopy (SEM). A novel analytical model, capable of predicting the influence of GO content on the elastic properties of the material, is obtained. Numerical simulations considering the experimental conditions are carried out. the full strain field given by the DIC system is successfully reproduced by means of the finite element method (FEM). While, the experimental failure is explained by the crack growth simulations using the eXtended finite element method (XFEM).
A Cuboid Model for Assessing Surface Soil Moisture
Authors: Zhu Xiufang , Pan Yaozhong , Wang Junxia , Liu Ying
Published: December 2019

This study proposes a cuboid model for soil moisture assessment. In the model, the three edges were the meteorological, soil, and vegetation feature parameters highly related to soil moisture, and the edge lengths represented the degree of influence of each feature parameter on soil moisture. Soil moisture is assessed by the cuboid diagonal, which is referred to as the cuboid soil moisture index (CSMI) in this paper. The model was applied and validated in the Huang-Huai-Hai Plain. The results showed that (1) the difference in land surface temperature between day and night (ΔLST), land surface water index (LSWI), and accumulated precipitation (AP) were most closely correlated with soil moisture observation data in our study area, and were therefore selected as soil, crop, and meteorological system parameters to participate in CSMI calculations, respectively. (2) CSMI-1, with a cuboid length coefficient of 2/1/2, was the best model. The correlation of soil moisture derived from CSMI-1 with observed values was 0.64, 0.60, and 0.52 at depths of 10 cm, 20 cm, and 50 cm, respectively. (3) CSMI-1 had good applicability to the evaluation of soil moisture under different vegetation coverage. When the normalized difference vegetation index (NDVI)was 0–0.7, CSMI-1 was highly correlated with soil moisture at a significance level of 0.01. (4) The three-dimensional (3D) CSMI model can be easily converted to a two-dimensional (2D) model to adapt to different surface conditions (as long as the weight coefficient of one parameter is set to 0). Irrigation information (if available) can be considered as artificial recharge precipitation added in the AP to improve the accuracy of soil moisture inversion. This study provides a reference for soil moisture inversion using optical remote sensing images by integrating soil, vegetation, and meteorological feature parameters.
Proximity Trade and Urban Sustainability: Small Retailers’ Expectations Towards Local Online Marketplaces
Authors: Delgado-de Miguel Juan-Francisco , Buil-López Menchero Tamar , Esteban-Navarro Miguel-Ángel , García-Madurga Miguel-Ángel
Published: December 2019

The aim of this research is to identify the expectations offered by small retail businesses towards local online selling platforms as an innovative tool to ensure their future and the urban sustainability. Based on the previous findings obtained from an analysis of trends, actors and marketplaces operating in the retail sector, sixty semi-structured in-depth interviews have been carried out to Spanish local retail managers and owners. Opportunities and risks faced by local online marketplaces are presented from the perspective of small retail stores. Different attitudes towards online shopping platforms have been identified depending on their size, presence and experience in the online world, which has allowed us to categorize local retail businesses in six groups. Despite the perceptual and attitudinal differences between them, it is concluded that merchants have assumed that the current and future business model goes through the digitalization of their businesses and the selling on e-commerce platforms. The coexistence of the e-marketplace and the physical stores, conducted by the same retailers, could have a positive effect on the urban sustainability: on the one hand, with the economic strengthening and renovation of the historical centers and, on the other, with the maintenance of the population and traditional social relationships.
Framework Conditions and Strategies for Pop-Up Environments in Urban Planning
Authors: Bertino Gaetano , Fischer Tatjana , Puhr Gustav , Langergraber Guenter , Österreicher Doris
Published: December 2019

Urban strategies and the way cities are planned have changed throughout history, adapting to the needs of the inhabitants, infrastructure requirements, and advances in technology. Uses and customs of people and cities are changing and can evolve much faster than in the past, with the result that urban planning is often too slow to adequately meet the current needs of society. In this context, the development of pop-up environments for temporary developments could be a solution to meet the needs of flexibility, adaptation, and resilience of a city. This allows the urban planner to consider systems from a short-term perspective, fulfilling current needs without compromising the development of potentially different activities in the future. The purpose of this research work is to outline the general requirements of pop-up environments in urban developments that allow for adequate integration into urban planning strategies. Based on an extensive evaluation of the existing literature and a series of case studies, the paper analyzes the key elements that define the framework conditions of urban planning strategies for temporary developments that generate a positive impact on the overall urban system.
Environmental Impact of the High Concentrator Photovoltaic Thermal 2000x System
Authors: Shittu Emmanuel , Kolokotroni Maria , Stojceska Valentina
Published: December 2019

High Concentrator Photovoltaic Thermal (HCPV/T) systems produce both electrical and thermal energy and they are efficient in areas with high Direct Normal Irradiance (DNI). This paper estimates the lifecycle environmental impact of the HCPV/T 2000x system for both electrical and thermal functionalities. Process-based attributional method following the guidelines and framework of ISO 14044/40 was used to conduct the Life Cycle Assessment (LCA). The midpoint and endpoint impact categories were studied. It was found that the main hotspots are the production of the thermal energy system contributing with 50% and 55%, respectively, followed by the production of the tracking system with 29% and 32% and the operation and maintenance with 13% and 7%. The main contributor to the lifecycle environmental impact category indicators was found to be the raw materials acquisition/production and manufacturing of the thermal energy and tracking systems. The results indicate that the lifecycle environmental impact of the HCPV/T 2000x system is lower compared to fuel-based Combined Heat and Power (CHP) and non-Renewable Energy Sources (non-RES) systems.
Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India
Authors: Singh Raghvendra Pratap , Manchanda Geetanjali , Yang Yingjie , Singh Dipti , Srivastava Alok Kumar , Dubey Ramesh Chandra , Zhang Chengsheng
Published: December 2019

The compatibility between rhizobia and legumes for nitrogen-fixing nodules and the stages of root hair curling, formation of infection thread, and nodulation initiation have been vitally studied, but the factors for the sustainable root surface colonization and efficient symbiosis within chickpea and rhizobia have been poorly investigated. Hence, we aimed to analyze phenotypic properties and phylogenetic relationships of root-nodule bacteria associated with chickpea (Cicer arietinum) in the north-west Indo Gangetic Plains (NW-IGP) region of Uttar Pradesh, India. In this study, 54 isolates were recovered from five agricultural locations. Strains exhibited high exopolysaccharide production and were capable of survival at 15–42 °C. Assays for phosphate solubilization, catalase, oxidase, Indole acetic acid (IAA) production, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity revealed that all the tested isolates possessed plant growth-promoting potential. Metabolic profiling using Biolog plates indicated that patterns of substrate utilization differed considerably among isolates. A biofilm formation assay showed that isolates displayed a nearly four-fold range in their capacity for biofilm development. Inoculation experiments indicated that all isolates formed nodules on chickpea, but they exhibited more than a two-fold range in symbiotic efficiency. No nodules were observed on four other legumes (Phaseolus vulgaris, Pisum sativum, Lens culinaris, and Vigna mungo). Concatenated sequences from six loci (gap, edD, glnD, gnD, rpoB, and nodC) supported the assignment of all isolates to the species Mesorhizobium ciceri, with strain M. ciceri Ca181 as their closest relative.
Making Future Teachers More Aware of Issues Related to Sustainability: An Assessment of Best Practices
Authors: Olmos-Gómez Mª del Carmen , Estrada-Vidal Ligia Isabel , Ruiz-Garzón Francisca , López-Cordero Rafael , Mohamed-Mohand Laila
Published: December 2019

The aim of the present research was to uncover the opinions of future teachers about whether the formative knowledge of education for sustainability received in seminars during their practicums improved their competencies in sustainability and their values in educational teaching for their future classroom practice. The study entailed qualitative research with a thematic analysis. Semi-structured interviews comprising 14 questions, which were validated by 15 experts, were used to solicit the opinions of 52 future teachers with respect to the importance of sustainability and the development of intercultural competencies among their students. The information from the interviews was analyzed using thematic analysis on the basis of education for sustainable development (ESD). We conclude that the teachers’ practicum placements and training experiences were very positive because a lecturing pedagogical approach, taught via practical application in a school environment by an expert of recognized prestige, can be a useful resource to develop awareness of both sustainable development and its education, as well as to learn didactic strategies to apply ESD, which addresses aspects that are relevant in multicultural contexts, such as tolerance and empathy.
Governing at Scale: Successful Local Food Initiatives in the World’s Cities
Authors: Warshawsky Daniel , Vos Robert
Published: December 2019

To introduce the special issue on local food initiatives in the world’s cities, this editorial examines the role of scale and the governance of local food initiatives in cities. The seven papers in this issue focus variously on food system governance at the scales of metropolitan regions, neighborhoods, households, and individual consumers. Although local food initiatives must work to overcome structural challenges operating at global and national scales, as delineated in key literature on food systems, taken together, the seven articles suggest that more sustainable outcomes are possible if local initiatives embrace change across multiple scales.
Equilibrium of Two-Dimensional Cycloidal Pantographic Metamaterials in Three-Dimensional Deformations
Authors: Scerrato Daria , Giorgio Ivan
Published: December 2019

A particular pantographic sheet, modeled as a two-dimensional elastic continuum consisting of an orthogonal lattice of continuously distributed fibers with a cycloidal texture, is introduced and investigated. These fibers conceived as embedded beams on the surface are allowed to be deformed in a three-dimensional space and are endowed with resistance to stretching, shearing, bending, and twisting. A finite element analysis directly derived from a variational formulation was performed for some explanatory tests to illustrate the behavior of the newly introduced material. Specifically, we considered tests on: (1) bias extension; (2) compressive; (3) shear; and (4) torsion. The numerical results are discussed to some extent. Finally, attention is drawn to a comparison with other kinds of orthogonal lattices, namely straight, parabolic, and oscillatory, to show the differences in the behavior of the samples due to the diverse arrangements of the fibers.
Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
Authors: Wang Xichun , Zhang Yafei , Zhao Jie , Cao Li , Zhu Lei , Huang Yingying , Chen Xiaofang , Rahman Sajid Ur , Feng Shibin , Li Yu , Wu Jinjie
Published: December 2019

The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of IL-1β and IL-6 were increased in the cells. The mRNA relative expression of NF-κB p65, IKKα, and IKKβ were upregulated with the increasing of DON concentration, while the relative expression of IκB-α mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells.
Genomic and Proteomic Characterization of Bacteriophage BH1 Spontaneously Released from Probiotic Lactobacillus rhamnosus Pen
Authors: Jarocki Piotr , Komoń-Janczara Elwira , Podleśny Marcin , Kholiavskyi Oleksandr , Pytka Monika , Kordowska-Wiater Monika
Published: December 2019

Lactobacillus rhamnosus Pen is a human endogenous strain used for the production of probiotic formula, which is effective in the prevention of antibiotic-associated diarrhoea. Our study showed that this probiotic strain releases bacteriophage BH1 without the addition of any inducing agent. Our research revealed that phage BH1 has a circular genome with a length of 40721 nt and a GC content of 44.8%. The genome of phage BH1 possesses 57 open reading frames which could be divided into functional modules associated with DNA packaging, morphogenesis, lysis, integration, genetic switch, and replication. In spite of similarity in morphology and genomic organization, comparative analysis revealed substantial genetic diversity and mosaic genomic architecture among phages described for the Lactobacillus casei group. Additionally, qPCR and ddPCR analysis confirmed earlier microscopic observations indicating that L. rhamnosus Pen liberates bacteriophage particles during growth. This occurs spontaneously, and is not a result of external inducing factors. For samples collected after 4 and 24 h of L. rhamnosus Pen culture, the number of attB and attP copies increased 2.5 and 12 times, respectively. This phenomenon, by introducing resistance to other phages or enhancing the biofilm-forming capabilities, may increase the survivability of microorganisms in their natural ecological niche. Conversely, spontaneous phage induction may be an important virulence factor for bacteria, posing a potential threat for the human host.