bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptome profiling of flax plants exposed to a low-frequency alternating electromagnetic field
Authors: Kostyn Kamil , Boba Aleksandra , Kozak Bartosz , Sztafrowski Dariusz , Widuła Jan , Szopa Jan , Preisner Marta
Published: June 2023

All living organisms on Earth evolved in the presence of an electromagnetic field (EMF), adapted to the environment of EMF, and even learned to utilize it for their purposes. However, during the last century, the Earth’s core lost its exclusivity, and many EMF sources appeared due to the development of electricity and electronics. Previous research suggested that the EMF led to changes in intercellular free radical homeostasis and further altered the expression of genes involved in plant response to environmental stresses, inorganic ion transport, and cell wall constituent biosynthesis. Later, CTCT sequence motifs in gene promoters were proposed to be responsible for the response to EMF. How these motifs or different mechanisms are involved in the plant reaction to external EMF remains unknown. Moreover, as many genes activated under EMF treatment do not have the CTCT repeats in their promoters, we aimed to determine the transcription profile of a plant exposed to an EMF and identify the genes that are directly involved in response to the treatment to find the common denominator of the observed changes in the plant transcriptome.
Incorporating lesion-to-lesion heterogeneity into early oncology decision making
Authors: Kumar Rukmini , Qi Timothy , Cao Yanguang , Topp Brian
Published: June 2023

RECISTv1.1 (Response Evaluation Criteria In Solid Tumors) is the most commonly used response grading criteria in early oncology trials. In this perspective, we argue that RECISTv1.1 is ambiguous regarding lesion-to-lesion variation that can introduce bias in decision making. We show theoretical examples of how lesion-to-lesion variability causes bias in RECISTv1.1, leading to misclassification of patient response. Next, we review immune checkpoint inhibitor (ICI) clinical trial data and find that lesion-to-lesion heterogeneity is widespread in ICI-treated patients. We illustrate the implications of ignoring lesion-to-lesion heterogeneity in interpreting biomarker data, selecting treatments for patients with progressive disease, and go/no-go decisions in drug development. Further, we propose that Quantitative Systems Pharmacology (QSP) models can aid in developing better metrics of patient response and treatment efficacy by capturing patient responses robustly by considering lesion-to-lesion heterogeneity. Overall, we believe patient response evaluation with an appreciation of lesion-to-lesion heterogeneity can potentially improve decision-making at the early stage of oncology drug development and benefit patient care.
Biofilms associated with ship submerged surfaces: implications for ship biofouling management and the environment
Authors: Georgiades Eugene , Scianni Chris , Tamburri Mario N.
Published: June 2023

Biofouling (including initial microbial biofilms) of submerged ship surfaces can directly impact vessel operations, leading to increases in fuel usage, greenhouse gas emissions, and the likelihood of non-indigenous species (NIS) transfer and impacts. Considerations of attainable and consistent biosecurity goals are paramount to the success of the widespread adoption of biofouling management policy. Proactive in-water cleaning (IWC) of biofilms from submerged ship surfaces may provide a viable option from a biosecurity and ship operations standpoint, however these benefits need to be balanced against other environmental costs, including the potential for increased biosecurity risks associated with the elevated release of diverse microbes from ship surfaces.
Extensive complex neocortical movement topography devolves to simple output following experimental stroke in mice
Authors: Wolsh Cassandra C. , Brown Rogers Milton , Brown Andrew R. , Pratt Gilbert Andrew , Boychuk Jeffery Allen
Published: June 2023

The neocortex encodes complex and simple motor outputs in all mammalian species that have been tested. Given that changes in neocortical reorganization (and corresponding corticospinal output) have been implicated in long term motor recovery after stroke injury, there remains a need to understand this biology in order to expedite and optimize clinical care. Here, changes in the neocortical topography of complex and simple movement outputs were evaluated in mice following experimental middle cerebral artery occlusion (MCAo). Neocortical motor output was defined using long-duration parameters of intracortical microstimulation (LD-ICMS) based on area and spatial coordinates of separate motor output types to build upon our recent report in uninjured mice. LD-ICMS test sites that elicited complex (multi-joint) movement, simple (single skeletal joint) movement, as well as co-elicited FORELIMB + HINDLIMB responses were detected and recorded. Forelimb reaching behavior was assessed using the single pellet reaching (SPR) task. At 6 weeks post-surgery, behavioral deficits persisted and neocortical territories for separate movements exhibited differences in neocortical area, and spatial location, and differed between MCAo-Injured animals (i.e., the MCAo group) and Sham-Injured animals (i.e., the Control group). MCAo-Injury reduced neocortical area of complex movements while increasing area of simple movements. Limited effects of injury were detected for spatial coordinates of neocortical movements. Significant positive correlations were detected between final SPR performance and either area of complex retract or area of co-occurring FORELIMB + HINDLIMB sites.
Rapid and sensitive determination of Se and heavy metals in foods using electrothermal vaporization inductively coupled plasma mass spectrometry with a novel transportation system
Authors: Lan Guanyu , Li Xue , Yao Jijun , Yu Xiaofeng , Liu Qinghai , Qiu Cheng , Mao Xuefei
Published: June 2023

Rapid, sensitive and simultaneous determination of trace multi-elements in various plant food samples such as grain, oilseed, vegetable and tea is always a challenge thus far. In this work, a rapid determination method for Se, Cd, As and Pb in food samples by inductively coupled plasma mass spectrometry (ICP-MS) using slurry sampling electrothermal vaporization (SS-ETV) was developed. To improve the analytical sensitivity and precision as well as eliminate the memory effect, a gas turbulator line and signal delay device (SDD) were for the first time designed for the graphite furnace (GF) ETV coupled with ICP-MS. The signal acquisition parameters of ICP-MS, ashing and vaporization conditions, and the flow rates of carrier gas and gas turbulator were investigated for Se, Cd, As and Pb in food samples. Under the optimized conditions, the limits of determination (LODs) for Se, Cd, As and Pb were 0.5 ng g−1, 0.3 ng g−1, 0.3 ng g−1 and 0.6 ng g−1, respectively; the limits of quantification (LOQs) for Se, Cd, As and Pb were 1.7 ng g−1, 1.0 ng g−1, 1.0 ng g−1 and 1.9 ng g−1, respectively; linearity (R2) in the range of 1 to 4,000 ng g−1 was >0.999 using the standard addition method. This method was used to analyze 5 CRMs including rice, tea and soybeans, and the concentrations detected by this method were within the range of the certified values. The recoveries of Se, Cd, As and Pb in plant food matrices including grain, oilseed, celery, spinach, carrot and tea samples were 86–118% compared to the microwave digestion ICP-MS method; and the relative standard deviations (RSDs) were 1.2–8.9% for real food sample analysis, proving a good precision and accuracy for the simultaneous determination of multi-elements. The analysis time was less than 3 min, slurry preparation time < 5 min without sample digestion process. The proposed direct slurry sampling ICP-MS method is thus suitable for rapid and sensitive determination of Se, Cd, As and Pb in food samples with advantages such as simplicity, green and safety, as well as with a promising application potential in detecting more elements to protect food safety and human health.
Case report: A de novo NSD2 truncating variant in a child with Rauch-Steindl syndrome
Authors: Yang Qi , Gong Di , Yi Shang , Luo Jingsi , Zhang Qinle
Published: June 2023

Wolf–Hirschhorn syndrome (WHS) is a rare genetic disorder caused by a heterozygous deletion on chromosome 4p16.3, which is called the WHS critical region (WHSC). The major features of this disorder, including “Greek warrior helmet” facies, delayed growth, intellectual disability, seizures, and skeletal abnormalities, are caused by the combined haploinsufficiency of multiple genes. The WHS candidate 1 (WHSC1) gene, also known as NSD2, is located in the WHSC and has been reported to associate with Rauch-Steindl syndrome (RSS,OMIM 619695). RSS is a highly heterogeneous disease characterized by mild developmental delay, prenatal-onset growth restriction, low body mass index, and characteristic facial features distinct from WHS. In this report, using whole exome sequencing (WES), we identified a novel de novo heterozygous NSD2 truncating variant in a 7-year-old Chinese girl with Rauch-Steindl syndrome, including failure to thrive, facial dysmorphisms, developmental delay, intellectual disability, and hypotonia. These findings further support that haploinsufficiency of NSD2 is necessary for WHS, and molecular genetic testing is more accurate to diagnose these patients. The novel variant uncovered in this study further expands the mutation spectrum of NSD2.
How to approach and take care of minor adolescents whose situations raise ethical dilemmas? a position paper of the European academy of pediatrics
Authors: Michaud Pierre-André , Takeuchi Yusuke-Leo , Mazur Artur , Hadjipanayis Adamos A. , Ambresin Anne-Emmanuelle
Published: June 2023

In the care of adolescents, health care providers often face situations raising ethical concerns or dilemmas, such as refusal of a treatment or hospitalization, or request of confidentiality while engaging in risky behaviors or facing unplanned pregnancy. This position paper provides concrete avenues as how to assess the adolescent's capacity for autonomous decision making, e.g. the patient's competence in a specific situation, and how to elicit informed choice or consent. To do so, professionals need to be sensitized and trained as how to assess the cognitive and socio-psychological development of the young patient. Another challenge for the health professionals is to balance the needs to support patient's autonomy while offering secure guidance and protection if needed. To optimize such a process, they establish a climate of trust and empathy that will allow the patient to participate freely in the decision. In addition, especially when the decisions have potentially important consequences on the health and life, the professionals include, with the adolescent's permission, parents, caregivers or other significant adults, as well as they may request the opinion of other members of the health care team or expert colleagues such as ethicists.
Randomized control trial of a childhood obesity prevention family-based program: “Abriendo Caminos” and effects on BMI
Authors: Teran-Garcia Margarita , Hammons Amber J. , Olvera Norma , Greder Kimberly , Plaza-Delrestre Maria , Andrade Flavia Cristina Drumond , Fiese Barbara , Wiley Angela R.
Published: June 2023

Background

Hispanic individuals are at increased risk for obesity and other chronic health conditions. This article evaluates the effect of a family-based, childhood obesity primary prevention intervention in a community setting.

Methods

A multi-site, randomized controlled trial community program with assessments at pre (T0), post-program (T1), and 6-months post-program (T2). Participating families were recruited from five sites. Only families of Mexican or Puerto Rican heritage with a least one child between 6 and 18 years were included in the study, without weight restrictions. Families were randomized to the intervention and control arms. Intervention families received six-2 h weekly workshops. Control families received printed generic nutrition and wellness information. Heights and weights were measured at the 3-time points to calculate BMI z-scores, BMI-percentiles, and weight status using age- and sex-specific growth charts, according to the CDC guidelines.

Results

There were no differences in BMI-z scores between children in the intervention (n = 239) and control groups (n = 187) at T0. BMI z-scores decreased in the intervention group (−0.03, 95% CI, −0.066, −0.003, p = 0.032) at T1, but not in the control group at T1. Changes in BMI z-scores were not statistically significant at T2.

Conclusion

The Abriendo Caminos intervention effectively prevented unhealthy weight gain in Hispanic children in the short-term, but not at 6-months post-intervention. Younger children and girls benefited more from the program at 6-months post-intervention. Additional efforts are needed to sustain long-term changes. Culturally-tailored programs can provide families with the knowledge to produce short-term changes and a potential pathway for sustainable changes in implementing healthy behaviors.
Comparison of bleeding risk and hypofibrinogenemia-associated risk factors between tigecycline with cefoperazone/sulbactam therapy and other tigecycline-based combination therapies
Authors: Zhang Lei , Cai Xinfeng , Peng Fangchen , Tian Shuangshuang , Wu Xinjing , Li Yun , Guo Jinlin
Published: June 2023

Background: Tigecycline and cefoperazone/sulbactam can cause coagulation disorders; tigecycline may also lead to hypofibrinogenemia, raising safety concerns. This study aimed to investigate whether tigecycline plus cefoperazone/sulbactam increases the risk of bleeding compared with other tigecycline-based combination therapies and identify risk factors for tigecycline-associated hypofibrinogenemia.

Methods: In this multi-method, multicenter, retrospective study, coagulation and other baseline variables were compared using a cohort study, and risk factors for hypofibrinogenemia using a case-control study.

Results: The 451 enrolled participants were divided into three group: tigecycline plus cefoperazone/sulbactam (Group A, 193 patients), tigecycline plus carbapenems (Group B, 200 patients) and tigecycline plus β-lactams without N-methylthio-tetrazole (NMTT) side chains (Group C, 58 patients). Activated partial thromboplastin time and prothrombin time were prolonged, and fibrinogen declined for all patients after tigecycline-based medication (all p < 0.05). Prothrombin time in Group B was significantly longer than in other groups (p < 0.05), but there were no significant differences in bleeding events between the three groups (p = 0.845). Age greater than 80 years (OR: 2.85, 95% CI: 1.07–7.60), treatment duration (OR: 1.29, 95% CI: 1.19–1.41), daily dose (OR: 2.6, 95% CI: 1.29–5.25), total bilirubin (OR: 1.01, 95% CI: 1.01–1.02) and basal fibrinogen (OR: 1.32, 95% CI: 1.14–1.63) were independent risk factors of hypofibrinogenemia. The optimal cut-off for treatment course was 6 days for high-dose and 11 days for low-dose.

Conclusion: Tigecycline plus cefoperazone/sulbactam did not increase the risk of bleeding compared with tigecycline plus carbapenem, or tigecycline plus β-lactam antibiotics without NMTT-side-chains. Coagulation function should be closely monitored in patients receiving tigecycline treatment.
Bryostatin-1: a promising compound for neurological disorders
Authors: Tian Zhen , Lu Xin-Tong , Jiang Xun , Tian Jiao
Published: June 2023

The central nervous system (CNS) is the most complex system in human body, and there is often a lack of effective treatment strategies for the disorders related with CNS. Natural compounds with multiple pharmacological activities may offer better options because they have broad cellular targets and potentially produce synergic and integrative effects. Bryostatin-1 is one of such promising compounds, a macrolide separated from marine invertebrates. Bryostatin-1 has been shown to produce various biological activities through binding with protein kinase C (PKC). In this review, we mainly summarize the pharmacological effects of bryostatin-1 in the treatment of multiple neurological diseases in preclinical studies and clinical trials. Bryostatin-1 is shown to have great therapeutic potential for Alzheimer’s disease, multiple sclerosis, fragile X syndrome, stroke, traumatic brain injury, and depression. It exhibits significant rescuing effects on the deficits of spatial learning, cognitive function, memory and other neurological functions caused by diseases, producing good neuroprotective effects. The promising neuropharmacological activities of bryostatin-1 suggest that it is a potential candidate for the treatment of related neurological disorders although there are still some issues needed to be addressed before its application in clinic.
Modelling exposure between populations using networks of mobility during COVID-19
Authors: Takko Tuomas , Bhattacharya Kunal , Kaski Kimmo
Published: June 2023

The use of mobile phone call detail records and device location data for the calling patterns, movements, and social contacts of individuals, have proven to be valuable for devising models and understanding of their mobility and behaviour patterns. In this study we investigate weighted exposure networks of human daily activities in the capital region of Finland as a proxy for contacts between postal code areas during the pre-pandemic year 2019 and pandemic years 2020, 2021 and early 2022. We investigate the suitability of gravity and radiation type models for reconstructing the exposure networks based on geo-spatial and population mobility information. For this we use a mobile phone dataset of aggregated daily visits from a postal code area to cellphone grid locations, and treat it as a bipartite network to create weighted one mode projections using a weighted co-occurrence function. We fit a classical gravity model and a radiation model to the averaged weekly and yearly projection networks with geo-spatial and socioeconomic variables of the postal code areas and their populations. We also consider an extended gravity type model comprising of additional postal area information such as distance via public transportation and population density. The results show that the co-occurrence of human activities, or exposure, between postal code areas follows both the gravity and radiation type interactions, once fitted to the empirical network. The effects of the pandemic beginning in 2020 can be observed as a decrease of the overall activity as well as of the exposure of the projected networks. These effects can also be observed in the network structure as changes towards lower clustering and higher assortativity. Evaluating the parameters of the fitted models over time shows on average a shift towards a higher exposure of areas in closer proximity as well as a higher exposure towards areas with larger population. In general, the results show that the postal code level networks changed to be more proximity weighted after the pandemic began, following the government imposed non-pharmaceutical interventions, with differences based on the geo-spatial and socioeconomic structure of the areas.
Cell wall response of field grown Populus to Septoria infection
Authors: Bryant Nathan , Muchero Wellington , Weber Rachel A. , Barros Jaime , Chen Jin-Gui , Tschaplinski Timothy J. , Pu Yunqiao , Ragauskas Arthur J.
Published: June 2023

Due to its ability to spread quickly and result in tree mortality,Sphaerulina musiva (Septoria)is one of the most severe diseases impactingPopulus. Previous studies have identified thatSeptoriainfection induces differential expression of phenylpropanoid biosynthesis genes. However, more extensive characterization of changes to lignin in response toSeptoriainfection is lacking. To study the changes of lignin due toSeptoriainfection, four field grown, naturally variantPopulus trichocarpaexhibiting visible signs ofSeptoriainfection were sampled at health, infected, and reaction zone regions for cell wall characterization. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and acid hydrolysis were applied to identify changes to the cell wall, and especially lignin. FTIR and subsequent principal component analysis revealed that infected and reaction zone regions were similar and could be distinguished from the non-infected (healthy) region. NMR results indicated the general trend that infected region had a higher syringyl:guaiacyl ratio and lowerp-hydroxybenzoate content than the healthy regions from the same genotype. Finally, Klason lignin content in the infected and/or reaction zone regions was shown to be higher than healthy region, which is consistent with previous observations of periderm development and metabolite profiling. These results provide insights on the response ofPopuluswood characteristics toSeptoriainfection, especially between healthy and infected region within the same genotype.
The ‘Matthew effect’ in Chinese kindergarten principals’ professional development: a mixed-methods study
Authors: Qian Yu , Zhangchen Shiyuan , Li Hui
Published: June 2023

Principals play a leading role in kindergarten quality improvement, and thus their needs for professional development (PD) should be understood and met. This national study adopted a mixed-methods approach to survey 3,065 kindergarten principals in China and interviewed 16 of them. First, the latent profile analysis of survey data yielded three profiles of PD needs: (1) low (7.5%), (2) medium (22.2%), and (3) high profiles (70.3%), indicating 70.3% of Chinese principals need PD badly. The high-profile group features ‘inexperienced principals working at newly established private kindergartens’, the most disadvantaged among the three groups. Second, ANOVA tests revealed significant rural–urban and public-private differences in Chinese principals’ professional backgrounds and PD needs. In particular, significant public-private and rural–urban differences were observed in the principals’ ‘current degree’ (Fs > 63, ps < 0.001) and ‘desired degree’ (Fs > 39, ps < 0.001). The rural principals aspired more than their urban counterparts to obtain ‘a higher degree” or ‘a certificate’ (ps < 0.05). Third, the follow-up interviews confirmed remarkable rural–urban and public-private gaps in PD needs, indicating a noticeable ‘Matthew effect’: the poor got less, whereas the rich got more. The implications for future PD policy and program development are discussed.
Age and sex-specific stroke epidemiology in COVID-19
Authors: Kim Youngran , Parekh Maria A. , Li Xiaojin , Huang Yan , Zhang Guo-Qiang , Manwani Bharti
Published: June 2023

Background

COVID-19 has emerged as an independent risk factor for stroke. We aimed to determine age and sex-specific stroke incidence and risk factors with COVID-19 in the US using a large electronic health record (EHR) that included both inpatients and outpatients.

Methods

A retrospective cohort study was conducted using individual-level data from Optum® de-identified COVID-19 EHR. A total of 387,330 individuals aged ≥ 18 with laboratory-confirmed COVID-19 between March 1, 2020 and December 31, 2020 were included. The primary outcome was cumulative incidence of stroke after COVID-19 confirmation within 180 days of follow-up or until death. Kaplan–Meier cumulative incidence curves for acute ischemic stroke (AIS), intracerebral hemorrhage (ICH), and a composite outcome of all strokes were stratified by sex and age, and the differences in curves were assessed using a log-rank test. The relative risk of stroke by demographics and risk factors was estimated using multivariable Cox-proportional hazards regressions and adjusted hazard ratios (aHRs).

Results

Of 387,330 COVID-19 patients, 2,752 patients (0.71%, 95% CI 0.68–0.74) developed stroke during the 180-day follow-up, AIS in 0.65% (95% CI 0.62–0.67), and ICH in 0.11% (95% CI 0.10–0.12). Of strokes among COVID-19 patients, 57% occurred within 3 days. Advanced age was associated with a substantially higher stroke risk, with aHR 6.92 (5.72–8.38) for ages 65–74, 9.42 (7.74–11.47) for ages 75–84, and 11.35 (9.20–14.00) for ages 85 and older compared to ages 18–44 years. Men had a 32% higher risk of stroke compared to women. African-American [aHR 1.78 (1.61–1.97)] and Hispanic patients [aHR 1.48 (1.30–1.69)] with COVID-19 had an increased risk of stroke compared to white patients.

Conclusion

This study has several important findings. AIS and ICH risk in patients with COVID-19 is highest in the first 3 days of COVID-19 positivity; this risk decreases with time. The incidence of stroke in patients with COVID-19 (both inpatient and outpatient) is 0.65% for AIS and 0.11% for ICH during the 180-day follow-up. Traditional stroke risk factors increase the risk of stroke in patients with COVID-19. Male sex is an independent risk factor for stroke in COVID-19 patients across all age groups. African-American and Hispanic patients have a higher risk of stroke from COVID-19.
Design and Experiment of a Low-Loss Harvesting Test Platform for Cabbage
Authors: Tong Wenyu , Zhang Jianfei , Cao Guangqiao , Song Zhiyu , Ning Xiaofeng
Published: June 2023

In order to explore the mechanism and influence mechanism of cabbage harvest damage, a low-loss cabbage harvest test platform was designed on the basis of combining the physical characteristics of cabbage with the mechanical characteristics of mechanical harvest and the cabbage harvest operation process. Through the design of key components of the test platform harvesting, the key parameters of the pulling-out device, the reel device, the flexible clamping and conveying device, and the double-disc cutting device were determined. The movement changes of cabbage during pulling out, conveying, and cutting were analyzed to clarify the process of damage generation and critical conditions of damage in cabbage harvesting operations. The test results showed that when the speed of the pulling out device was controlled at 80–120 r/min, the speed of the clamping and conveying device was controlled at 120–240 r/min, and the speed of the double disc cutter was controlled at 140–180 r/min, the average success rate of pulling on the low-loss harvesting test platform was 92.7%; the average damage rate of the pulling process was 7.32%; the average success rate of clamping and conveying was 88.6%; the average damage rate of the clamping and conveying link was 12%; the average success rate of root cutting was 89.3%; and the average damage rate of the cutting link was 11.34%. The average qualified rate of harvesting in the pulling link was 86.7%, the average qualified rate of harvesting in the clamping and conveying link was 75.3%, and the average qualified rate of harvesting in the cutting link was 77.3%. All the performance indicators meet the design requirements and relevant standards, and the research results can provide a reference for the development and structural improvement of low-loss harvesting equipment for cabbage.
Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (Camellia sinensis (L.) Kuntze)
Authors: Shen Yaozong , Wang Hui , Han Xiao , Fan Kai , Shen Jiazhi , Li He , Ding Shibo , Song Dapeng , Wang Yu , Ding Zhaotang
Published: June 2023

C. sinensis is an economically important crop for tea production that experiences increasing demand and good export potential. Therefore, crops need to be expanded, and high-quality planting material is required. Vegetative propagation by cuttings is the prevalent method; therefore, this paper explored its optimization potential modeled on cultivar ‘Jiukengzao’. This study wanted to deeply explore blue-light-induced adventitious root formation and development of tea cuttings, so we conducted short-term (0 h, 8 h, and 16 h) and long-term (30 d, 60 d, and 90 d) time-course analyses on tea cutting seedlings. Short-term, full-length transcriptome analysis showed that the expression of genes related to plant hormone signal transduction and auxin transport was highest at 16 h. Sixteen hours of light was considered as suitable for adventitious root growth and development of tea cuttings. Long-term phytohormone analysis showed that the trend of indole-3-carboxylic acid (ICA) change was: 60 d > 90 d > 30 d. Long-term, full-length transcriptome analysis showed that the gene expression trends in K2, K5, K6, and K8 clusters were: 90 d > 60 d > 30 d, and the opposite was observed in K1, K4, and K11 clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of the genes in these seven clusters are involved in “plant hormone signal transduction (ko04075)”. This includes auxin early responsive protein AUX/IAA, auxin response factor ARF, auxin-responsive protein SAUR, etc. In addition, genes related to auxin transport and synthesis were identified as PIN1, 3, 4, PILS2, 6, 7, flavin-containing monooxygenase YUC9, and YUC10, and the expression trend of these genes was mostly consistent with the change trend of ICA content. This study further explained the molecular mechanism of blue-light-induced adventitious root formation and development of tea cuttings. It is recommended that blue light can be used to promote the adventitious root growth and development of tea cuttings in practical production.
Constitution of a Camelina sativa L. Synthetic Population and Agronomic Comparison between Spring and Winter Cultivation in North Italy
Authors: Ghidoli Martina , Frazzini Sara , De Benedetti Stefano , Sangiorgio Stefano , Landoni Michela , Scarafoni Alessio , Rossi Luciana , Pilu Roberto
Published: June 2023

In recent years, the interest in increasingly sustainable agriculture has also turned attention towards new cover crops suitable for use in marginal areas that could enter the food chain as new protein and oil sources or for biodiesel production. In this scenario, Camelina sativa is a perfect crop to study. Camelina is an annual herbaceous plant belonging to the Brassicaceae which is interesting in terms of its oil content, since the seeds contain about 40% oils, with a high level of polyunsaturated fatty acids (30–40% alpha linolenic acid, 15–25% linoleic acid, 15% oleic acid and about 15% eicosenoic acid). It is a hexaploid species (2n = 40, genome size ~782 Mb) characterized by rapid growth, a short life cycle (85–100 days for spring varieties, 190–210 for autumn varieties) and low input cultivation needs. However, its use in feed and food is limited by the presence of glucosinolates (GLS). GLS are sulfur molecules involved in plant defense. In recent years, they have been studied not only as antinutritionals but also for their anti-carcinogenic effects against chronic inflammatory and heart diseases and for their use as natural pesticides. Given the recent interest in camelina and its highly nutritious oil, eight pure lines and a synthetic population were compared in two different growing periods, spring and winter. In this work, the genetic materials were characterized for different phenotypic traits, yields and yield components, and bromatological and glucosinolate content. The results confirmed that in North Italy, camelina has higher yields if cultivated in the autumn–winter period (about 2 t/ha vs. 0.6 t/ha); furthermore, a negative correlation was found between spring and winter yields, indicating that varieties that produce more in winter cultivation produce less in spring cultivation. Moreover, to our knowledge, it is the first work in which a synthetic population of Camelina sativa has been tested and proved to be a valid solution for use in various environments both for its adaptability and for the low content of glucosinolates (about 17 mmol/kg).
Effect of Group Housing of Preweaned Dairy Calves: Health and Fecal Commensal Antimicrobial Resistance Outcomes
Authors: Breen Martin J. , Williams Deniece R. , Abdelfattah Essam M. , Karle Betsy M. , Byrne Barbara A. , Lehenbauer Terry W. , Aly Sharif S.
Published: June 2023

The objectives of this study were to investigate the effects of group housing (three calves per group) on bovine respiratory disease (BRD), diarrhea and antimicrobial resistance (AMR) to fecal commensal Escherichia coli (EC) and enterococci/streptococci (ES). Our study comprised two arms, one experimental and one observational. In the experimental arm, preweaned calves on a California dairy were randomized to either individual (IND; n = 21) or group (GRP; n = 21) housing, using a modified California-style wooden hutch. The study period lasted from birth to 56 days of age, during which calves were health scored daily. Cumulative incidence and hazard ratios were estimated for disease. Antimicrobial resistance outcomes were assessed using a prospective cohort design; feces were collected from each calf three times per week and EC and ES were evaluated for AMR using the broth microdilution method against a panel of 19 antimicrobial drugs (AMD). Analysis of treatment records was used to select calves that had been exposed (EXP) to an AMD-treated calf. In GRP, exposure occurred when a calf was a hutchmate with an AMD-treated calf. In IND, exposure occurred when a calf was a neighbor with an AMD-treated calf (TRT). Age-matched unexposed calves (UNEXP) were then selected for comparison. Proportions of AMR in fecal commensals among EXP, UNEXP, and TRT calves were compared between GRP and IND. Accelerated failure time survival regression models were specified to compare differences in minimum inhibitory concentration (MIC) of fecal commensals between EXP and UNEXP calves within each of GRP and IND calves separately. Group calves had a BRD hazard 1.94 times greater that of IND calves (p = 0.03), using BRD treatment records as the outcome. For AMR in EC isolates, higher resistance to enrofloxacin was detected in enrofloxacin-EXP GRP isolates compared with enrofloxacin-EXP IND isolates, and UNEXP GRP calves had lower resistance to ceftiofur compared with enrofloxacin-EXP and enrofloxacin-TRT calves. A significant housing-by-time interaction was detected for EC ceftiofur MIC in EXP GRP calves at 4–14 days post exposure to enrofloxacin (MIC EXP-UNEXP: µg/mL (95% CI): 10.62 (1.17, 20.07)), compared with UNEXP calves. The findings of this study show an increase in BRD hazard for group-housed calves and an increase in ceftiofur resistance in group-housed calves exposed to an enrofloxacin-treated calf.
Pharmacological Modulation of Energy and Metabolic Pathways Protects Hearing in the Fus1/Tusc2 Knockout Model of Mitochondrial Dysfunction and Oxidative Stress
Authors: Tan Winston J. T. , Santos-Sacchi Joseph , Tonello Jane , Shanker Anil , Ivanova Alla V.
Published: June 2023

Tightly regulated and robust mitochondrial activities are critical for normal hearing. Previously, we demonstrated that Fus1/Tusc2 KO mice with mitochondrial dysfunction exhibit premature hearing loss. Molecular analysis of the cochlea revealed hyperactivation of the mTOR pathway, oxidative stress, and altered mitochondrial morphology and quantity, suggesting compromised energy sensing and production. Here, we investigated whether the pharmacological modulation of metabolic pathways using rapamycin (RAPA) or 2-deoxy-D-glucose (2-DG) supplementation can protect against hearing loss in female Fus1 KO mice. Additionally, we aimed to identify mitochondria- and Fus1/Tusc2-dependent molecular pathways and processes critical for hearing. We found that inhibiting mTOR or activating alternative mitochondrial energetic pathways to glycolysis protected hearing in the mice. Comparative gene expression analysis revealed the dysregulation of critical biological processes in the KO cochlea, including mitochondrial metabolism, neural and immune responses, and the cochlear hypothalamic–pituitary–adrenal axis signaling system. RAPA and 2-DG mostly normalized these processes, although some genes showed a drug-specific response or no response at all. Interestingly, both drugs resulted in a pronounced upregulation of critical hearing-related genes not altered in the non-treated KO cochlea, including cytoskeletal and motor proteins and calcium-linked transporters and voltage-gated channels. These findings suggest that the pharmacological modulation of mitochondrial metabolism and bioenergetics may restore and activate processes critical for hearing, thereby protecting against hearing loss.
Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)
Authors: Li Yanan , Chen Zongfu , Zhang Peng , Gao Feng , Wang Junfeng , Lin Li , Zhang Haibin
Published: June 2023

At present, deep-sea enzymes are a research hotspot. In this study, a novel copper–zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 °C, and it maintains high activity in the range of 0–60 °C. It also has high thermal stability when incubated at 37 °C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4–11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.
Role of NADPH Oxidase 4 in Corneal Endothelial Cells Is Mediated by Endoplasmic Reticulum Stress and Autophagy
Authors: Ma Dae Joong , Hwang Jin Sun , Noh Kyung Bo , Oh Sun-Hee , Kim Kyoung Wook , Shin Young Joo
Published: June 2023

Human corneal-endothelial cells (hCEnCs) are located on the inner layer of the cornea. Injury to CEnCs leads to permanent corneal edema, requiring corneal transplantation. NADPH oxidase 4 (NOX4) has been reported to be implicated in the pathogenesis of CEnCs diseases. Thus, we investigated the role of NOX4 in CEnCs in this study. In an animal study, siRNA for NOX4 (siNOX4) or plasmid for NOX4 (pNOX4) was introduced into the corneal endothelium of rats by electroporation, using a square-wave electroporator (ECM830, Havard apparatus) to decrease or increase the expression of NOX4, respectively, and the rat corneas were cryoinjured through contact with a metal rod of 3 mm diameter frozen in liquid nitrogen for 10 min. The immunofluorescence staining of NOX4 and 8-OHdG showed that the levels of NOX4 and 8-OHdG were decreased in the siNOX4 group compared to the siControl, and increased in the pNOX4 group compared to the pControl at one week after treatment. Without cryoinjury, corneal opacity was more severe, and the density of CEnCs was lower, in pNOX4-treated rats compared to pControl. After cryoinjury, the corneas were more transparent, and the CEnC density was higher, in siNOX4-treated rats. The hCEnCs were cultured and transfected with siNOX4 and pNOX4. The silencing of NOX4 in hCEnCs resulted in a normal cell shape, higher viability, and higher proliferation rate than those transfected with the siControl, while NOX4 overexpression had the opposite effect. NOX4 overexpression increased the number of senescent cells and intracellular oxidative stress levels. NOX4 overexpression increased ATF4 and ATF6 levels, and nuclear translocation of XBP-1, which is the endoplasmic reticulum (ER) stress marker, while the silencing of NOX4 had the opposite effect. Additionally, the mitochondrial membrane potential was hyperpolarized by the silencing of NOX4, and depolarized by NOX4 overexpression. The LC3II levels, a marker of autophagy, were decreased by the silencing of NOX4, and increased by NOX4 overexpression. In conclusion, NOX4 plays a pivotal role in the wound-healing and senescence of hCEnCs, by modulating oxidative stress, ER stress, and autophagy. The regulation of NOX4 may be a potential therapeutic strategy for regulating the homeostasis of CEnCs, and treating corneal-endothelial diseases.
A High Power Factor LED Driver with Intrinsic Current Balancing Capability
Authors: Cheng Hung-Liang , Hung Yi-Chan
Published: June 2023

The research proposed a novel LED driver with the functions of power-factor correction (PFC) and current balancing. A flyback converter and a Class-D series resonant converter were integrated by sharing an active switch to form a single-stage circuit topology. The flyback converter played the role of a PFC circuit. The component parameters were designed to make the flyback converter to operate at discontinuous-conduction mode (DCM). In this way, the input line current can be sinusoidal, resulting in near unity power factor and low total harmonic distortion in current (THDi). The resonant converter was connected in series with a differential-mode transformer with a turns ratio of 1 to drive four LED strings. The current of the four LED strings will be automatically and evenly balanced by using the 1:1 transformer. This article analyzed the different modes of operation in detail, derived the mathematical equations and designed the parameters of the circuit components. Finally, a 72-W prototype LED driver was implemented and tested. A satisfactory performance has verified the feasibility of the proposed LED driver.
An Automatic Tomato Growth Analysis System Using YOLO Transfer Learning
Authors: Fukada Keita , Hara Kataru , Cai Jingyong , Teruya Daichi , Shimizu Ikuko , Kuriyama Takatsugu , Koga Katsumi , Sakamoto Kosuke , Nakamura Yoshiyuki , Nakajo Hironori
Published: June 2023

In recent years, Japan’s agricultural industry has faced a number of challenges, including a decline in production due to a decrease in farmland area, a shortage of labor due to a decrease in the number of producers, and an aging population. Therefore, in recent years, smart agriculture using robots and IoT has been studied. A caliper is often used to analyze the growth of tomatoes in a plant factory, but this method may damage the stems and is also hard on the measurer. We developed a system that detects them through image analysis and measures the thickness of stems and the length between flower clusters and growing points. The camera device developed in this study costs about USD 150 and once installed, it does not need to be moved unless it malfunctions. The camera device reduces the effort required to analyze crop growth by about 80%.
Determination of the Depth of Cut via Surface Integrity
Authors: Gavrus Cristina , Ivan Nicolae-Valentin , Oancea Gheorghe
Published: June 2023

The present paper continues the authors’ research in machining process optimization, including the direction of machining parameters optimization. The paper develops an innovative method, via surface integrity, for determining the technological route and the related depths of cut, with respect to machining front faces of cast iron parts. For correctly establishing the depths of the cut, the errors that appear within surface and subsurface layers during the casting process, as well as during machining, must be gradually eliminated. These errors make it necessary to consider the concept of surface integrity. This paper presents the modality of integrating the components of surface integrity into the depth of cut. For the practical use of this method, a new software tool based on a series of mathematical models and a small database was conceived. A case study illustrates how the method is applied and the software tool used to solve a specific application in the case of a belt pulley.
A Group Decision-Making Approach in MCDM: An Application of the Multichoice Best–Worst Method
Authors: Ahmad Qazi Shoeb , Khan Mohammad Faisal , Ahmad Naeem
Published: June 2023

Multicriteria decision-making (MCDM) techniques have successfully been used to address a wide range of real-world decision-making issues. The best–worst method (BWM) is one of the several deterministic MCDM approaches. A recently proposed method called the multichoice best–worst method (MCBWM) takes into account several linguistic terms for pairwise comparisons of relative preferences among the criteria. It has been shown that the MCBWM approach has advantages over BWM: it reduces the calculation and determines optimal weight values by providing the choices for the optimal solution. This paper proposes a unique method for group decision-making based on MCBWM. We extended the MCBWM to solve group decision-making problems. A novel solution approach was developed and validated for multiple problems. Two examples and one case study were solved using the proposed approach to demonstrate the validity and application of the proposed method. The results were further compared with existing models to validate the proposed approach. We found that the obtained ranking order for all problems is the same and that the proposed model has a higher consistency ratio than the existing approaches. This method can be extended to other mathematical programming models for collective decision making in uncertain situations.
Hybrid Precoding Design for Subarray-Structure-Enabled mmWave URLLC System
Authors: Liu Zhiren , Shen Yuan , Qiang Mengye , Wang Gang , Wang Jun-Bo
Published: June 2023

Ultra-reliable low-latency communication (URLLC) is an important application scenario in fifth generation (5G) communication. With the increasing deployment of 5G, spectrum resources are becoming increasingly scarce, and millimeter wave (mmWave) operating in a high frequency has garnered significant attention. However, the short wavelength of the mmWave makes the signals susceptible to fading. Precoding has emerged as a promising solution for mitigating the severe path loss in mmWave, which can enhance the system capacity and communication performance. In our system, an achievable rate maximization problem is established by jointly optimizing the analog and digital precoders at the base station, subject to the constraint of the total power and the constant modulus constraint of the subarrays. The simulation results demonstrate that the algorithm employed in our system can successfully solve the optimization problem in this new scenario. Furthermore, the subarray-structure hybrid precoder has a higher energy efficiency than that of a fully connected hybrid precoder or all-digital precoder. The proposed algorithm also outperforms the MM (Majorization–Minimization) algorithm.
Mesh-Free MLS-Based Error-Recovery Technique for Finite Element Incompressible Elastic Computations
Authors: Kahla Nabil Ben , AlQadhi Saeed , Ahmed Mohd.
Published: June 2023

The finite element error and adaptive analysis are implemented in finite element procedures to increase the reliability of numerical analyses. In this paper, the mesh-free error-recovery technique based on moving least squares (MLS) interpolation is applied to recover the errors in the stresses and displacements of incompressible elastic finite element solutions and errors are estimated in energy norms. The effects of element types (triangular and quadrilateral elements) and the formation of patches (mesh-free patch, mesh-dependent element-based patch, and mesh-dependent node-based patch) for error recovery in MLS and conventional least-square interpolation-error quantification are also assessed in this study. Numerical examples of incompressible elasticity, including a problem with singularity, are studied to display the effectiveness and applicability of the mesh-free MLS interpolation-error recovery technique. The mixed formulation (displacement and pressure) is adopted for a finite element analysis of the incompressible elastic problem. The rate of convergence, the effectivity of the error estimation, and modified meshes for desired accuracy are used to assess the effectiveness of the error estimators. The error-convergence rates are computed in the original FEM solution, in the post-processed solution using mesh-free MLS-based displacement, stress recovery, mesh-dependent patch-based least-square-based displacement, and stress recovery (ZZ) as (0.9777, 2.2501, 2.0012, 1.6710 and 1.5436), and (0.9736, 2.0869, 1.6931, 1.8806 and 1.4973), respectively, for four-node quadrilateral, and six-node triangular meshes. It is concluded that displacement-based recovery was more effective in the finite element incompressible elastic analysis than stress-based recovery using mesh-free and mesh-dependent patches.