bluuebunny/crossref_metadata_2025 huggingface dataset:
Learning-based personalisation of robot behaviour for robot-assisted therapy
Authors: Stolarz Michał , Mitrevski Alex , Wasil Mohammad , Plöger Paul G.
Published: April 2024

During robot-assisted therapy, a robot typically needs to be partially or fully controlled by therapists, for instance using a Wizard-of-Oz protocol; this makes therapeutic sessions tedious to conduct, as therapists cannot fully focus on the interaction with the person under therapy. In this work, we develop a learning-based behaviour model that can be used to increase the autonomy of a robot’s decision-making process. We investigate reinforcement learning as a model training technique and compare different reward functions that consider a user’s engagement and activity performance. We also analyse various strategies that aim to make the learning process more tractable, namely i) behaviour model training with a learned user model, ii) policy transfer between user groups, and iii) policy learning from expert feedback. We demonstrate that policy transfer can significantly speed up the policy learning process, although the reward function has an important effect on the actions that a robot can choose. Although the main focus of this paper is the personalisation pipeline itself, we further evaluate the learned behaviour models in a small-scale real-world feasibility study in which six users participated in a sequence learning game with an assistive robot. The results of this study seem to suggest that learning from guidance may result in the most adequate policies in terms of increasing the engagement and game performance of users, but a large-scale user study is needed to verify the validity of that observation.
Robotic misinformation in dementia care: emotions as sense-making resources in residents’ encounters with robot animals
Authors: Persson Marcus , Thunman Elin , Iversen Clara , Redmalm David
Published: April 2024

Robot animals, designed to mimic living beings, pose ethical challenges in the context of caring for vulnerable patients, specifically concerning deception. This paper explores how emotions become a resource for dealing with the misinformative nature of robot animals in dementia care homes. Based on observations of encounters between residents, care workers, and robot animals, the study shows how persons with dementia approach the ambiguous robots as either living beings, material artifacts, or something in-between. Grounded in interactionist theory, the research demonstrates that emotions serve as tools in the sense-making process, occurring through interactions with the material object and in collaboration with care workers. The appreciation of social robots does not solely hinge on them being perceived as real or fake animals; persons with dementia may find amusement in “fake” animals and express fear of “real” ones. This observation leads us to argue that there is a gap between guidelines addressing misinformation and robots and the specific context in which the technology is in use. In situations where small talk and play are essential activities, care workers often prioritize responsiveness to residents rather than making sure that the robot’s nature is transparent. In these situations, residents’ emotional expressions serve not only as crucial resources for their own sense-making but also as valuable indicators for care workers to comprehend how to navigate care situations.
The surprising role of marital status on sport second-screening: demographic influences during the 2022 world cup viewing in Israel
Authors: Galily Yair , Samuel-Azran Tal , Laor Tal
Published: April 2024

To better understand what characterizes those who use a second screen while watching sport, the study examine a variety of demographic factors influencing browsing device trends before, during (“second screen”), and after sports games. It does so by utilizing survey data from Israeli viewers of the 2022 World Cup using a convenience sample (N = 242). In line with our hypotheses, those with higher education and higher reported income were more likely to browse devices for information around and during games. Against our hypothesis, young adults were less likely to engage in browsing before, during and after the games, possibly because they tend to watch games with friends or in public places. Divorced and single individuals are more likely to engage in multi-platform browsing and second-screening during sport games vs. married participants, who tended to watch the games with friends or in public places. The results are the first to indicate the important role of marital status in second-screening during sport games. Overall, they depict a picture of the average second-screener as a non-married older male with higher income and education, thus indicating that higher intellect combined with non-marital status, thus potentially more spare time as well as possibly higher levels of loneliness and during games are linked to sport second-screening. The results are the first to highlight the important role of marital status over young age on the tendency to second screen during sport games.
COVID-free surgical pathways for treating patients with acute calculous cholecystitis: a retrospective comparative study
Authors: Giacopelli Pietro , Cristaudi Alessandra , Majno Pietro , Roesel Raffaello , Iaquinandi Fabiano , Mongelli Francesco
Published: April 2024

Introduction

During the Sars-Cov-2 crisis, some of the resources committed to emergency surgery services were transiently reallocated to the care of patients with severe COVID-19, preserving immediate treatment of mostly non-deferrable conditions. Moreover, the fear of contracting infections or hindering the treatment of critical COVID-19 patients has caused many individuals to defer seeking emergency care. This situation has then possibly modified the standard of care of some common surgical conditions and the relative outcomes. Our aims was to highlight any difference in surgical outcomes in patients treated for acute cholecystitis before and during the COVID-19 outbreak.

Method

This is a retrospective study on a prospectively collected database that included all consecutive patients treated for acute cholecystitis from March 2019 to February 2021 at the Lugano Regional Hospital, a COVID-free hospital for general surgery patients. Patients were divided into pre-and post-COVID-19 outbreak groups. We collected thorough clinical characteristics and intra-and postoperative outcomes.

Results

We included 124 patients, of which 60 and 64 were operated on before and after the COVID-19 outbreak respectively. The two groups resulted similar in terms of patients' clinical characteristics (age, gender, body mass index, ASA score, and comorbidities). Patients in the post-outbreak period were admitted to the hospital 0.7 days later than patients in the pre-outbreak period (3.8 ± 6.0 days vs. 3.1 ± 4.1 days, p = 0.453). Operative time, recovery room time, complications, and reoperations resulted similar between groups. More patients in the post-outbreak period received postoperative antibiotic therapy (63.3% vs. 37.5%, p = 0.004) and for a longer time (6.9 ± 5.1 days vs. 4.5 ± 3.9 days, p = 0.020). No significant histopathological difference was found in operatory specimens.

Discussion

Despite more frequent antibiotic therapy that suggests eventually worse inflammatory local status, our results showed similar outcomes for patients treated for acute cholecystitis before and during the COVID-19 pandemic. The local COVID management, reallocating resources, and keeping COVID-free hospitals was key to offering patients a high standard of treatment.
Pilot cadaveric study on the feasibility of cricothyroidotomy and the associated complications in 30 cats
Authors: Delle Cave Julia A. , Larcheveque Samuel R. , Martin Edouard , O’Toole Elizabeth
Published: April 2024

Objectives

The study’s primary goal was to assess the feasibility of the cricothyroidotomy technique (CTT) in cats and evaluate its success rate (i.e., secure airway access). Secondary outcomes were the assessment of the subjective difficulty of airway access based on body score condition and weight. Further secondary outcomes consisted of procedural time and scoring of associated complications. The current study hypothesized that the CTT procedure would provide secure airway access with a reasonable success rate.

Materials and methods

A prospective experimental study assessing the performance of CTT and associated complications was conducted on 30 feline cadavers. A procedural datasheet was completed to subjectively grade difficulty of landmark palpation, guide placement and tube placement and expected success of the procedure. A dissection was then performed post-procedure by a blinded observer to evaluate for any associated damages.

Results

CTT was successful in securing an airway in 100% of the cats. The time to completion of the CTT was rapid, with a median time of 49 s (ranging from 31 to 90 s) for securing an airway. Of importance, this procedure was judged to be overall easy (median “ease of procedure score” of 7/10; ranging from 3 to 10) by the experimenters. The post-procedural lesion rate was elevated (76.7%) in this population of cats, though based on the lesion scores, was deemed mild in 73.9% of the cases.

Clinical significance

CTT warrants consideration as the primary option for emergency front-of-neck airway access for cats although further studies are necessary.
Peer Texting to Promote Quitline Use and Smoking Cessation Among Rural Participants in Vietnam: Randomized Clinical Trial
Authors: Sadasivam Rajani S. , Nagawa Catherine S. , Wijesundara Jessica G. , Flahive Julie , Nguyen Hoa L. , Larkin Celine , Faro Jamie M. , Balakrishnan Kavitha , Ha Duc Anh , Nguyen Cuong Kieu , Vuong Anh , Phan Phuong Thu , Pham Quyen Phi Li , Allison Jeroan J. , Houston Thomas Karr
Published: April 2024

Objectives: We tested an adapted version of an effective U.S.-based peer-texting intervention to promote Quitline use and smoking cessation among rural participants in Vietnam.

Methods: We conducted a two-arm randomized trial with participants recruited at four rural community centers. The intervention included peer messages sent for six months that promoted Quitline use and smoking cessation. Additionally, biweekly two-way text messages assessed participants’ interest in Quitline referral and current smoking status. Comparison participants received only the bi-weekly text message assessment of their current smoking status. At six months, we assessed Quitline use and smoking cessation. Smoking cessation was assessed using the 7-day point prevalence question and verified with a carbon monoxide breath monitor (<=6 ppm).

Results: Among 750 participants, the intervention had higher Quitline verified use (18%, 95% CI 0.14, 0.22) than comparison (1%, 95% CI .2, 2, p < 0.0001). Carbon-monoxide-verified smoking cessation did not differ between the two groups. However, intervention (28.3%, 95% CI) and comparison (28.1%, 95% CI) participants had substantial rates of carbon monoxide cessation at 6 months (both 28%).

Conclusion: Our study highlighted the promise of texting interventions to extend tobacco control efforts in Vietnam.
Disulfiram, an Anti-alcoholic Drug, Targets Macrophages and Attenuates Acute Rejection in Rat Lung Allografts
Authors: Yoshiyasu Nobuyuki , Matsuki Rei , Sato Masaaki , Urushiyama Hirokazu , Toda Etsuko , Terasaki Yasuhiro , Suzuki Masaki , Shinozaki-Ushiku Aya , Terashima Yuya , Nakajima Jun
Published: April 2024

Macrophages contribute to post-transplant lung rejection. Disulfiram (DSF), an anti-alcoholic drug, has an anti-inflammatory effect and regulates macrophage chemotactic activity. Here, we investigated DSF efficacy in suppressing acute rejection post-lung transplantation. Male Lewis rats (280–300 g) received orthotopic left lung transplants from Fisher 344 rats (minor histocompatibility antigen-mismatched transplantation). DSF (0.75 mg/h) monotherapy or co-solvent only (50% hydroxypropyl-β-cyclodextrin) as control was subcutaneously administered for 7 days (n = 10/group). No post-transplant immunosuppressant was administered. Grades of acute rejection, infiltration of immune cells positive for CD68, CD3, or CD79a, and gene expression of monocyte chemoattractant protein and pro-inflammatory cytokines in the grafts were assessed 7 days post-transplantation. The DSF-treated group had significantly milder lymphocytic bronchiolitis than the control group. The infiltration levels of CD68+ or CD3+ cells to the peribronchial area were significantly lower in the DSF than in the control groups. The normalized expression of chemokine ligand 2 and interleukin-6 mRNA in allografts was lower in the DSF than in the control groups. Validation assay revealed interleukin-6 expression to be significantly lower in the DSF than in the control groups. DSF can alleviate acute rejection post-lung transplantation by reducing macrophage accumulation around peripheral bronchi and suppressing pro-inflammatory cytokine expression.
The Modeling and Control of a Distributed-Vector-Propulsion UAV with Aero-Propulsion Coupling Effect
Authors: Xia Jiyu , Zhou Zhou
Published: April 2024

A novel distributed-vector-propulsion UAV (DVPUAV) is introduced in this paper, which has the capability of Vertical takeoff and landing (VTOL), and can realize relatively high-speed cruise. As the core of the DVPUAV, the propulsion wing designed under the guidance of the integration idea is not only a lifting body but also a propulsion device and a control mechanism. However, this kind of aircraft has a series of difficult problems with complex aero-propulsion coupling, flight modes switching, and so many inputs and control coupling. In order to describe this coupling effect to improve the accuracy of dynamics, an aero-propulsion coupling model is developed, considering both computation reliability and real-time. Afterward, a unique control framework is designed for the DVPUAV. By optimizing control logic, this control framework realizes the decoupling of longitudinal and lateral directional control and even the decoupling of roll and yaw control. Next, based on the Iterative linear quadratic regulator (ILQR), a new Model Predictive Control (MPC) controller with the ability to solve complex nonlinear problems is proposed which achieves the unification of the controller for the full flight envelope. Finally, the good performance of the control framework and controller is verified in the whole process of the flight simulation from take-off to landing.
A Combined Experimental and Numerical Calibration Approach for Modeling the Performance of Aerospace-Grade Titanium Alloy Products
Authors: Tuninetti Víctor , Sepúlveda Héctor , Beecher Carlos , Rojas-Ulloa Carlos , Oñate Angelo , Medina Carlos , Valenzuela Marian
Published: April 2024

Finite element modeling for designing and optimizing lightweight titanium aerospace components requires advanced simulation tools with adequate material modeling. In this sense, a hybrid strategy is proposed in this work to identify the parameters of the Johnson–Cook plasticity and damage laws using a combined direct-inverse method. A direct calibration method for plasticity law is applied based on the literature-reported data of strain-stress curves from experimental tensile tests at different temperatures and strain rates. The triaxiliaty-dependent fracture parameters of the Johnson–Cook damage law at reference conditions of strain rate and temperature (d1, d2, and d3) are calibrated with the direct method based on new data of experimental evolution of computed average fracture strain with the average stress triaxiality. The validation is performed with numerical results from an accurate micromechanics-based Ti64 model. The inverse calibration method is used to determine the strain rate and temperature-dependent damage parameters (d4 and d5) through large strain simulations of uniaxial tensile tests. The numerical results, including average strain and necking profile at fracture, are then utilized to calculate stress triaxiality by the Bridgman criterion for adjusting parameters d4 and d5. The calibrated model yields a 2.1% error for plasticity and 3.4% for fracture predictions. The experimental and simulated load-bearing capacity using the micromechanics damage model differed by only 1%. This demonstrates that the SC11–TNT model of Ti64 is reliable for identifying the Johnson–Cook damage law through the accurate use of inverse methods. The hybrid calibration strategy demonstrates the potential capability of the identified Johnson–Cook model to accurately predict the design load-carrying capacity of Ti64 aerospace components under different deformation rates and temperatures while accounting for material damage effects.
Synthesis, Characterization, and Evaluation of the Antimicrobial Effects and Cytotoxicity of a Novel Nanocomposite Based on Polyamide 6 and Trimetaphosphate Nanoparticles Decorated with Silver Nanoparticles
Authors: de Morais Leonardo Antônio , Souza Neto Francisco Nunes de , Hosida Thayse Yumi , dos Santos Danilo Martins , de Almeida Bianca Carvalho , Frollini Elisabete , Filho Sergio Paulo Campana , Barbosa Debora de Barros , de Camargo Emerson Rodrigues , Delbem Alberto Carlos Botazzo
Published: April 2024

This study aimed to develop a polymeric matrix of polyamide-6 (P6) impregnated with trimetaphosphate (TMP) nanoparticles and silver nanoparticles (AgNPs), and to evaluate its antimicrobial activity, surface free energy, TMP and Ag+ release, and cytotoxicity for use as a support in dental tissue. The data were subjected to statistical analysis (p < 0.05). P6 can be incorporated into TMP without altering its properties. In the first three hours, Ag+ was released for all groups decorated with AgNPs, and for TMP, the release only occurred for the P6-TMP-5% and P6-TMP-10% groups. In the inhibition zones, the AgNPs showed activity against both microorganisms. The P6-TMP-2.5%-Ag and P6-TMP-5%-Ag groups with AgNPs showed a greater reduction in CFU for S. mutans. For C. albicans, all groups showed a reduction in CFU. The P6-TMP groups showed higher cell viability, regardless of time (p < 0.05). The developed P6 polymeric matrix impregnated with TMP and AgNPs demonstrated promising antimicrobial properties against the tested microorganisms, making it a potential material for applications in scaffolds in dental tissues.
Antioxidant Actions of Melatonin: A Systematic Review of Animal Studies
Authors: Monteiro Karla Krislane Alves Costa , Shiroma Marcos Eiji , Damous Luciana Lamarão , Simões Manuel de Jesus , Simões Ricardo dos Santos , Cipolla-Neto José , Baracat Edmund Chada , Soares-Jr. Jose Maria
Published: April 2024

Melatonin is an indoleamine with crucial antioxidant properties that are used to combat inflammatory and neoplastic processes, as well as control transplants. However, the clinical applications of melatonin have not yet been fully consolidated in the literature and require in-depth analysis. Objectives: This study reviewed the literature on the antioxidant properties of melatonin in rat models. Methods: We followed the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses and used the PubMed, LILACS, and Cochrane databases, Google Scholar, and article references, irrespective of publication time. Results: Ten articles involving 485 rats were selected, and the effects of melatonin on antioxidant markers were investigated. Melatonin increased superoxide dismutase in nine studies, glutathione peroxidase in seven studies, and catalase in five studies. In contrast, melatonin reduced glutathione in three studies and malonaldehyde in seven of eight studies. Conclusion: Our findings suggest that melatonin effectively reduces oxidative stress.
Relgraph: A Multi-Relational Graph Neural Network Framework for Knowledge Graph Reasoning Based on Relation Graph
Authors: Tian Xin , Meng Yuan
Published: April 2024

Multi-relational graph neural networks (GNNs) have found widespread application in tasks involving enhancing knowledge representation and knowledge graph (KG) reasoning. However, existing multi-relational GNNs still face limitations in modeling the exchange of information between predicates. To address these challenges, we introduce Relgraph, a novel KG reasoning framework. This framework introduces relation graphs to explicitly model the interactions between different relations, enabling more comprehensive and accurate handling of representation learning and reasoning tasks on KGs. Furthermore, we design a machine learning algorithm based on the attention mechanism to simultaneously optimize the original graph and its corresponding relation graph. Benchmark and experimental results on large-scale KGs demonstrate that the Relgraph framework improves KG reasoning performance. The framework exhibits a certain degree of versatility and can be seamlessly integrated with various traditional translation models.
Satisfiability Threshold of Random Propositional S5 Theories
Authors: Su Zaihang , Wang Yisong , Feng Renyan , Zhou Chan
Published: April 2024

Modal logic S5, which isan important knowledge representation and reasoning paradigm, has been successfully applied in various artificial-intelligence-related domains. Similar to the random propositional theories in conjunctive clause form, the phase transition plays an important role in designing efficient algorithms for computing models of propositional S5 theories. In this paper, a new form of S5 formula is proposed, which fixes the number of modal operators and literals in the clauses of the formula. This form consists of reduced 3-3-S5 clauses of the form l1∨l2∨ξ, where ξ takes the form □(l3∨l4∨l5), ◊(l3∧l4∧l5), or a propositional literal, and li(1≤i≤5) is a classical literal. Moreover, it is demonstrated that any S5 formula can be translated into a set of reduced 3-3-S5 clauses while preserving its satisfiability. This work further investigates the probability of a random 3-c-S5 formula with c=1,2,3 being satisfied by random assignment. In particular, we show that the satisfiability threshold of random 3-1-S5 clauses is −ln2(1−Pd−Ps)ln78+Ps·ln34, where Ps and Pd denote the probabilities of different modal operators appearing in a clause. Preliminary experimental results on random 3-1-S5 formulas confirm this theoretical threshold.
Covariance and Uncertainty Principle for Dispersive Pulse Propagation
Authors: Cohen Leon
Published: April 2024

We develop the concept of covariance for waves and show that it plays a fundamental role in understanding the evolution of a propagating pulse. The concept clarifies several issues regarding the spread of a pulse and the motion of the mean. Exact results are obtained for the time dependence of the covariance between position and wavenumber and the covariance between position and group velocity. We also derive relevant uncertainty principles for waves.
Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers
Authors: Costantini Erica , Aielli Lisa , Gualdi Giulio , Baronio Manuela , Monari Paola , Amerio Paolo , Reale Marcella
Published: April 2024

Venous leg ulcers are one of the most common nonhealing conditions and represent an important clinical problem. The application of pulsed radiofrequency electromagnetic fields (PRF-EMFs), already applied for pain, inflammation, and new tissue formation, can represent a promising approach for venous leg ulcer amelioration. This study aims to evaluate the effect of PRF-EMF exposure on the inflammatory, antioxidant, cell proliferation, and wound healing characteristics of human primary dermal fibroblasts collected from venous leg ulcer patients. The cells’ proliferative and migratory abilities were evaluated by means of a BrdU assay and scratch assay, respectively. The inflammatory response was investigated through TNFα, TGFβ, COX2, IL6, and IL1β gene expression analysis and PGE2 and IL1β production, while the antioxidant activity was tested by measuring GSH, GSSG, tGSH, and GR levels. This study emphasizes the ability of PRF-EMFs to modulate the TGFβ, COX2, IL6, IL1β, and TNFα gene expression in exposed ulcers. Moreover, it confirms the improvement of the proliferative index and wound healing ability presented by PRF-EMFs. In conclusion, exposure to PRF-EMFs can represent a strategy to help tissue repair, regulating mediators involved in the wound healing process.
Intestinal Barrier Dysfunction and Gut Microbiota in Non-Alcoholic Fatty Liver Disease: Assessment, Mechanisms, and Therapeutic Considerations
Authors: Long Changrui , Zhou Xiaoyan , Xia Fan , Zhou Benjie
Published: April 2024

Non-alcoholic fatty liver disease (NAFLD) is a type of metabolic stress liver injury closely related to insulin resistance (IR) and genetic susceptibility without alcohol consumption, which encompasses a spectrum of liver disorders ranging from simple hepatic lipid accumulation, known as steatosis, to the more severe form of steatohepatitis (NASH). NASH can progress to cirrhosis and hepatocellular carcinoma (HCC), posing significant health risks. As a multisystem disease, NAFLD is closely associated with systemic insulin resistance, central obesity, and metabolic disorders, which contribute to its pathogenesis and the development of extrahepatic complications, such as cardiovascular disease (CVD), type 2 diabetes mellitus, chronic kidney disease, and certain extrahepatic cancers. Recent evidence highlights the indispensable roles of intestinal barrier dysfunction and gut microbiota in the onset and progression of NAFLD/NASH. This review provides a comprehensive insight into the role of intestinal barrier dysfunction and gut microbiota in NAFLD, including intestinal barrier function and assessment, inflammatory factors, TLR4 signaling, and the gut–liver axis. Finally, we conclude with a discussion on the potential therapeutic strategies targeting gut permeability and gut microbiota in individuals with NAFLD/NASH, such as interventions with medications/probiotics, fecal transplantation (FMT), and modifications in lifestyle, including exercise and diet.
So Shiho Tang Reduces Inflammation in Lipopolysaccharide-Induced RAW 264.7 Macrophages and Dextran Sodium Sulfate-Induced Colitis Mice
Authors: He Mei Tong , Park Geonha , Park Do Hwi , Choi Minsik , Ku Sejin , Go Seung Hyeon , Lee Yun Gyo , Song Seok Jun , Ahn Chang-Wook , Jang Young Pyo , Kang Ki Sung
Published: April 2024

So Shiho Tang (SSHT) is a traditional herbal medicine commonly used in Asian countries. This study evaluated the anti-inflammatory effect of SSHT and the associated mechanism using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and murine dextran sodium sulfate (DSS)-induced ulcerative colitis models. Pre-treatment of RAW 264.7 macrophages with SSHT significantly reduced LPS-induced inflammation by decreasing nitrite production and regulating the mitogen-activated protein kinase pathway. Meanwhile, in mice, DSS-induced colitis symptoms, including colon shortening and body weight loss, were attenuated by SSHT. Moreover, representative compounds of SSHT, including glycyrrhizic acid, ginsenoside Rb1, baicalin, saikosaponin A, and saikosaponin B2, were quantified, and their effects on nitrite production were measured. A potential anti-inflammatory effect was detected in LPS-induced RAW 264.7 cells. Our findings suggest that SSHT is a promising anti-inflammatory agent. Its representative components, including saikosaponin B2, ginsenoside Rb1, and baicalin, may represent the key active compounds responsible for eliciting the anti-inflammatory effects and can, therefore, serve as quality control markers in SSHT preparations.
Baicalin Weakens the Virulence of Porcine Extraintestinal Pathogenic Escherichia coli by Inhibiting the LuxS/AI-2 Quorum-Sensing System
Authors: Zong Bingbing , Xiao Yong , Wang Peiyi , Liu Wei , Ren Mingxing , Li Changyan , Fu Shulin , Zhang Yanyan , Qiu Yinsheng
Published: April 2024

Porcine extraintestinal pathogenic Escherichia coli (ExPEC) is a pathogenic bacterium that causes huge economic losses to the pig farming industry and considerably threatens human health. The quorum sensing (QS) system plays a crucial role in the survival and pathogenesis of pathogenic bacteria. Hence, it is a viable approach to prevent ExPEC infection by compromising the QS system, particularly the LuxS/AI-2 system. In this study, we investigated the effects of baicalin on the LuxS/AI-2 system of ExPEC. Baicalin at concentrations of 25, 50, and 100 μg/mL significantly diminished the survival ability of ExPEC in hostile environments and could inhibit the biofilm formation and autoagglutination ability in ExPEC. Moreover, baicalin dose-dependently decreased the production of AI-2 and down-regulated the expression level of luxS in PCN033. These results suggest that baicalin can weaken the virulence of PCN033 by inhibiting the LuxS/AI-2 system. After the gene luxS was deleted, AI-2 production in PCN033 was almost completely eliminated, similar to the effect of baicalin on the production of AI-2 in PCN033. This indicates that baicalin reduced the production of AI-2 by inhibiting the expression level of luxS in ExPEC. In addition, the animal experiment further showed the potential of baicalin as a LuxS/AI-2 system inhibitor to prevent ExPEC infection. This study highlights the potential of baicalin as a natural quorum-sensing inhibitor for therapeutic applications in preventing ExPEC infection by targeting the LuxS/AI-2 system.
MicroRNAs in Atrial Fibrillation: Mechanisms, Vascular Implications, and Therapeutic Potential
Authors: Vardas Emmanouil P. , Theofilis Panagiotis , Oikonomou Evangelos , Vardas Panos E. , Tousoulis Dimitris
Published: April 2024

Atrial fibrillation (AFib), the most prevalent arrhythmia in clinical practice, presents a growing global health concern, particularly with the aging population, as it is associated with devastating complications and an impaired quality of life. Its pathophysiology is multifactorial, including the pathways of fibrosis, inflammation, and oxidative stress. MicroRNAs (miRNAs), small non-coding RNA molecules, have emerged as substantial contributors in AFib pathophysiology, by affecting those pathways. In this review, we explore the intricate relationship between miRNAs and the aforementioned aspects of AFib, shedding light on the molecular pathways as well as the potential diagnostic applications. Recent evidence also suggests a possible role of miRNA therapeutics in maintenance of sinus rhythm via the antagonism of miR-1 and miR-328, or the pharmacological upregulation of miR-27b and miR-223-3p. Unraveling the crosstalk between specific miRNA profiles and genetic predispositions may pave the way for personalized therapeutic approaches, setting the tone for precision medicine in atrial fibrillation.
Molecular Mechanisms of Ischemic Stroke: A Review Integrating Clinical Imaging and Therapeutic Perspectives
Authors: Rehman Sana , Nadeem Arsalan , Akram Umar , Sarwar Abeer , Quraishi Ammara , Siddiqui Hina , Malik Muhammad Abdullah Javed , Nabi Mehreen , Ul Haq Ihtisham , Cho Andrew , Mazumdar Ishan , Kim Minsoo , Chen Kevin , Sepehri Sadra , Wang Richard , Balar Aneri B. , Lakhani Dhairya A. , Yedavalli Vivek S.
Published: April 2024

Ischemic stroke poses a significant global health challenge, necessitating ongoing exploration of its pathophysiology and treatment strategies. This comprehensive review integrates various aspects of ischemic stroke research, emphasizing crucial mechanisms, therapeutic approaches, and the role of clinical imaging in disease management. It discusses the multifaceted role of Netrin-1, highlighting its potential in promoting neurovascular repair and mitigating post-stroke neurological decline. It also examines the impact of blood–brain barrier permeability on stroke outcomes and explores alternative therapeutic targets such as statins and sphingosine-1-phosphate signaling. Neurocardiology investigations underscore the contribution of cardiac factors to post-stroke mortality, emphasizing the importance of understanding the brain–heart axis for targeted interventions. Additionally, the review advocates for early reperfusion and neuroprotective agents to counter-time-dependent excitotoxicity and inflammation, aiming to preserve tissue viability. Advanced imaging techniques, including DWI, PI, and MR angiography, are discussed for their role in evaluating ischemic penumbra evolution and guiding therapeutic decisions. By integrating molecular insights with imaging modalities, this interdisciplinary approach enhances our understanding of ischemic stroke and offers promising avenues for future research and clinical interventions to improve patient outcomes.
Novel Biomaterials in Glaucoma Treatment
Authors: Klézlová Adéla , Bulíř Petr , Klápšťová Andrea , Netuková Magdaléna , Šenková Kateřina , Horáková Jana , Studený Pavel
Published: April 2024

Glaucoma is a significant cause of blindness worldwide, and its treatment remains challenging. The disease progressively leads to damage to the optic disc and thus loss of visual acuity and visual field. High intraocular pressure (IOP) is a common risk factor. There are three major methods to treat this disease: topical, laser, and surgical. None of these are completely satisfactory; therefore, alternatives using new biomaterials are being sought. Since biomaterial engineering has experienced significant growth in recent decades, its products are gradually being introduced to various branches of medicine, with the exception of ophthalmology. Biomaterials, such as glaucoma drainage implants, have been successfully used to treat glaucoma. There is significant ongoing research on biomaterials as drug delivery systems that could overcome the disadvantages of topical glaucoma treatment, such as poor intraocular penetration or frequent drug administration. This article summarizes the use of novel biomaterials for glaucoma treatment presented in the literature. The literature search was based on articles published in English on PubMed.gov, Cochranelibrary.com, and Scopus.com between 2018 and 2023 using the following term “biomaterials in glaucoma.” A total of 103 published articles, including twenty-two reviews, were included. Fifty-nine articles were excluded on the basis of their titles and abstracts.
Higher Synovial Immunohistochemistry Reactivity of IL-17A, Dkk1, and TGF-β1 in Patients with Early Psoriatic Arthritis and Rheumatoid Arthritis Could Predict the Use of Biologics
Authors: Pinto-Tasende Jose A. , Fernandez-Moreno Mercedes , Rego Perez Ignacio , Fernandez-Lopez J. Carlos , Oreiro-Villar Natividad , De Toro Santos F. Javier , Blanco-García Francisco J.
Published: April 2024

Background: Delay in diagnosis and therapy in patients with arthritis commonly leads to progressive articular damage. The study aimed to investigate the immunohistochemical reactivity of synovial cytokines associated with inflammation and the bone erosives/neoformatives processes among individuals diagnosed with psoriatic arthritis (PsA), rheumatoid arthritis (RA), osteoarthritis (OA), and radiographic axial spondyloarthritis (r-axSpA), with the intention of identifying potential biomarkers. Methods: Specimens were collected from the inflamed knee joints of patients referred for arthroscopic procedures, and the synovial tissue (ST) was prepared for quantifying protein expression through immunohistochemical analysis (% expressed in Ratio_Area-Intensity) for TGF-β1, IL-17A, Dkk1, BMP2, BMP4, and Wnt5b. The collected data underwent thorough analysis and examination of their predictive capabilities utilising receiver operating characteristic (ROC) curves. Results: Valid synovial tissue samples were acquired from 40 patients for IHC quantification analysis. Initially, these patients had not undergone treatment with biologics. However, after 5 years, 4 out of 13 patients diagnosed with PsA and two out of nine patients diagnosed with RA had commenced biologic treatments. Individuals with early PsA who received subsequent biologic treatment exhibited significantly elevated IHC reactivity in ST for TGF-β1 (p = 0.015). Additionally, patients with both PsA and RA who underwent biologic therapy displayed increased IHC reactivity for IL-17A (p = 0.016), TGF-β1 (p = 0.009), and Dkk1 (p = 0.042). ROC curve analysis of IHC reactivity for TGF-β1, Dkk1, and IL-17A in the synovial seems to predict future treatment with biologics in the next 5 years with the area under the curve (AUC) of a combined sum of the three values: AUC: 0.828 (95% CI: 0.689–0.968; p 0.005) S 75% E 84.4%. Conclusions: Higher synovial immunohistochemistry reactivity of IL-17A, Dkk1, and TGF-β1 in patients with early psoriatic arthritis and rheumatoid arthritis may serve as potential indicators for predicting the necessity of utilising biologic treatments.
Analysing the Combined Effects of Radiotherapy and Chemokine Receptor 5 Antagonism: Complementary Approaches to Promote T Cell Function and Migration in Oesophageal Adenocarcinoma
Authors: Davern Maria , O’ Donovan Cillian , Donlon Noel E. , Mylod Eimear , Gaughan Caoimhe , Bhardwaj Anshul , Sheppard Andrew D. , Bracken-Clarke Dara , Butler Christine , Ravi Narayanasamy , Donohoe Claire L. , Reynolds John V. , Lysaght Joanne , Conroy Melissa J.
Published: April 2024

The presence of an immunosuppressive tumour microenvironment in oesophageal adenocarcinoma (OAC) is a major contributor to poor responses. Novel treatment strategies are required to supplement current regimens and improve patient survival. This study examined the immunomodulatory effects that radiation therapy and chemokine receptor antagonism impose on T cell phenotypes in OAC with a primary goal of identifying potential therapeutic targets to combine with radiation to improve anti-tumour responses. Compared with healthy controls, anti-tumour T cell function was impaired in OAC patients, demonstrated by lower IFN-γ production by CD4+ T helper cells and lower CD8+ T cell cytotoxic potential. Such diminished T cell effector functions were enhanced following treatment with clinically relevant doses of irradiation. Interestingly, CCR5+ T cells were significantly more abundant in OAC patient blood compared with healthy controls, and CCR5 surface expression by T cells was further enhanced by clinically relevant doses of irradiation. Moreover, irradiation enhanced T cell migration towards OAC patient-derived tumour-conditioned media (TCM). In vitro treatment with the CCR5 antagonist Maraviroc enhanced IFN-γ production by CD4+ T cells and increased the migration of irradiated CD8+ T cells towards irradiated TCM, suggesting its synergistic therapeutic potential in combination with irradiation. Overall, this study highlights the immunostimulatory properties of radiation in promoting anti-tumour T cell responses in OAC and increasing T cell migration towards chemotactic cues in the tumour. Importantly, the CCR5 antagonist Maraviroc holds promise to be repurposed in combination with radiotherapy to promote anti-tumour T cell responses in OAC.
Co-Expression of Niemann-Pick Type C1-Like1 (NPC1L1) with ACE2 Receptor Synergistically Enhances SARS-CoV-2 Entry and Fusion
Authors: Elste James , Cast Nicole , Udawatte Shalini , Adhikari Kabita , Payen Shannon Harger , Verma Subhash C. , Shukla Deepak , Swanson-Mungerson Michelle , Tiwari Vaibhav
Published: April 2024

The entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into human embryonic kidney (HEK293T) cells has been shown to be a cholesterol-rich, lipid raft-dependent process. In this study, we investigated if the presence of a cholesterol uptake receptor Niemann-pick type c1-like1 (NPC1L1) impacts SARS-CoV-2 cell entry. Initially, we utilized reporter-based pseudovirus cell entry assays and a spike (S) glycoprotein-mediated cell-to-cell fusion assay. Using Chinese hamster ovary (CHO-K1) cells, which lack endogenous receptors for SARS-CoV-2 entry, our data showed that the co-expression of NPC1L1 together with the ACE2 receptor synergistically increased SARS-CoV-2 pseudovirus entry even more than the cells expressing ACE-2 receptor alone. Similar results were also found with the HEK293T cells endogenously expressing the ACE2 receptor. Co-cultures of effector cells expressing S glycoprotein together with target cells co-expressing ACE-2 receptor with NPC1L1 significantly promoted quantitative cell-to-cell fusion, including syncytia formation. Finally, we substantiated that an elevated expression of NPC1L1 enhanced entry, whereas the depletion of NPC1L1 resulted in a diminished SARS-CoV-2 entry in HEK293T-ACE2 cells using authentic SARS-CoV-2 virus in contrast to their respective control cells. Collectively, these findings underscore the pivotal role of NPC1L1 in facilitating the cellular entry of SARS-CoV-2. Importance: Niemann-Pick type C1-like1 (NPC1L1) is an endosomal membrane protein that regulates intracellular cholesterol trafficking. This protein has been demonstrated to play a crucial role in the life cycle of several clinically important viruses. Although SARS-CoV-2 exploits cholesterol-rich lipid rafts as part of its viral entry process, the role of NPC1L1 in SARS-CoV-2 entry remains unclear. Our research represents the first-ever demonstration of NPC1L1’s involvement in facilitating SARS-CoV-2 entry. The observed role of NPC1L1 in human kidney cells is not only highly intriguing but also quite relevant. This relevance stems from the fact that NPC1L1 exhibits high expression levels in several organs, including the kidneys, and the fact that kidney damages are reported during severe cases of SARS-CoV-2. These findings may help us understand the new functions and mechanisms of NPC1L1 and could contribute to the identification of new antiviral targets.
Modeling of the Effect of Subperiosteal Hydrostatic Pressure Conductivity between Joints on Decreasing Contact Loads on Cartilage and of the Effect of Myofascial Relief in Treating Trigger Points: The Floating Skeleton Theory
Authors: Pitkin Mark R.
Published: April 2024

Chronic overloading of the cartilage can lead to its irreversible destruction, as observed in people with osteoarthritis. The floating skeleton model previously introduced postulates that overloading begins and progresses when a joint is isolated from the hydrostatical connection with other joints. Such a connection occurs via the interstitial fluid in subperiosteal space and allows for pressure transmission between synovial capsules modulating intra-articular pressure. In the current study, a simple experiment was performed to model an obstruction in the subperiosteal hydrostatic pressure conductivity between joints to illustrate the effect of that obstruction on loads borne by the joint. When the obstruction was removed, the load experienced by the joint was reduced as it was redistributed throughout the model structure. The experiment demonstrated that contact pressures can be redistributed when the conditions of Pascal’s Law are met.
Maximum Power Point Tracking of Photovoltaic Generation System Using Improved Quantum-Behavior Particle Swarm Optimization
Authors: Yu Gwo-Ruey , Chang Yong-Dong , Lee Weng-Sheng
Published: April 2024

This study introduces an improved quantum-behavior particle swarm optimization (IQPSO), tailored for the task of maximum power point tracking (MPPT) within photovoltaic generation systems (PGSs). The power stage of the MPPT system comprises a series of buck-boost converters, while the control stage contains a microprocessor executing the biomimetic algorithm. Leveraging the series buck-boost converter, the MPPT system achieves optimal operation at the maximum power point under both ideal ambient conditions and partial shade conditions (PSCs). The proposed IQPSO addresses the premature convergence issue of QPSO, enhancing tracking accuracy and reducing tracking time by estimating the maximum power point and adjusting the probability distribution. Employing exponential decay, IQPSO facilitates a reduction in tracking time, consequently enhancing convergence efficiency and search capability. Through single-peak experiments, multi-peak experiments, irradiance-changing experiments, and full-day experiments, it is demonstrated that the tracking accuracy and tracking time of IQPSO outperform existing biomimetic algorithms, such as the QPSO, firefly algorithm (FA), and PSO.
Simulation and Structural Analysis of a Flexible Coupling Bionic Desorption Mechanism Based on the Engineering Discrete Element Method
Authors: Li Jinguang , Qi Hongyan , Ma Yunhai , Gao Peng , Wu Baoguang
Published: April 2024

Soil adhesion is one of the important factors affecting the working stability and quality of agricultural machinery. The application of bionic non-smooth surfaces provides a novel idea for soil anti-adhesion. The parameters of sandy loam with 21% moisture content were calibrated by the Engineering Discrete Element Method (EDEM). The final simulated soil repose angle was highly consistent with the measured soil repose angle, and the obtained regression equation of the soil repose angle provides a numerical reference for the parameter calibration of different soils. By simulating the sinusoidal swing of a sandfish, it was found that the contact interface shows the phenomenon of stress concentration and periodic change, which reflects the effectiveness of flexible desorption and soil anti-adhesion. The moving resistance of the wedge with different wedge angles and different serrated structures was simulated. Finally, it was found that a 40° wedge with a high-tail sparse staggered serrated structure on the surface has the best drag reduction effect, and the drag reduction is about 10.73%.
Study on the Tensile and Shear Performances of Fully Precast Partially Composite Floor Slab Joints
Authors: Zhang Yongsheng , Chang Kanghui , Yang Yukun , Zhao Guangchen , Liu Yanjun
Published: April 2024

This study explored the tensile and shear characteristics of fully prefabricated partially composite floor slab joints through the design and testing of two tensile specimens, three steel–concrete specimens, and three concrete–concrete shear specimens. These tests aimed to evaluate how various connection designs influence the joints’ load-bearing capabilities and failure patterns. The findings revealed that the tensile specimens predominantly showed bond failures at the interface of the precast and cast-in-place layers, accompanied by rebar pull-out. Incorporating reinforcing bars or sleeves was found to potentially increase their ultimate load-bearing capacity by about 20%. The shear failures in the steel–concrete specimens were primarily due to interactions between the steel beam and adjacent composite slab, whereas the concrete–concrete specimens mostly underwent local crushing at the load application point and failure at the bonding interface. These observations affirmed the accuracy of the existing methods for calculating tensile and shear strengths, offering vital insights for the architectural design and construction of such floor joints.