bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuropsychological Function and Quality of Life after Resection of Suspected Lower-Grade Glioma in the Face Primary Motor Area
Authors: Stålnacke Mattias , Bergenheim Tommy , Sjöberg Rickard L.
Published: February 2021

The negative side effects of neurosurgical resection of the lower third of the primary motor cortex (M1) are often described as relatively mild. However, detailed descriptions of how these resections affect neurocognitive function, speech, mental health and quality of life (QoL) are sparse. In the present study, seven patients with suspected lower-grade glioma (WHO II-III) in the inferior M1 were assessed for facial motor function, cognitive function, anxiety and QoL before and after awake surgical resections. The main finding was that after surgery, six of the seven patients experienced a mild facial motor dysfunction, mainly affecting the mouth, tongue and throat. At the group level, we were also able to observe a significant postoperative decline in maximum verbal speed, whereas no negative effects on measures of word production (i.e., verbal fluency) were seen. Self-reported QoL data suggest that some patients experienced increased social isolation postoperatively but do not lend support to the interpretation that this was caused by direct neurological side effects of the surgery. The results appear to support the general notion that awake surgery in the lower M1 can be performed safely and with postoperative deficits that are most often perceived by the patient as tolerable.
Elucidation of Novel Therapeutic Targets for Breast Cancer with ESR1-CCDC170 Fusion
Authors: Jeong Jae Heon , Yun Jae Won , Kim Ha Young , Heo Chan Yeong , Lee Sejoon
Published: February 2021

Among the various types of breast cancer, the luminal B subtype is the most common in young women, and ESR1-CCDC170 (E:C) fusion is the most frequent oncogenic fusion driver of the luminal B subtype. Nevertheless, treatments targeting E:C fusion has not been well established yet. Hence, the aim of this study is to investigate potential therapies targeting E:C fusion based on systematic bioinformatical analysis of the Cancer Genome Atlas (TCGA) data. One thousand related genes were extracted using transcriptome analysis, and major signaling pathways associated with breast cancer were identified with over-representation analysis. Then, we conducted drug-target network analysis based on the OncoKB and CIViC databases, and finally selected potentially applicable drug candidates. Six major cancer-related signaling pathways (p53, ATR/ATM, FOXM1, hedgehog, cell cycle, and Aurora B) were significantly altered in E:C fusion-positive cases of breast cancer. Further investigation revealed that nine genes (AURKB, HDAC2, PLK1, CENPA, CHEK1, CHEK2, RB1, CCNA2, and MDM2) in coordination with E:C fusion were found to be common denominators in three or more of these pathways, thereby making them promising gene biomarkers for target therapy. Among the 21 putative actionable drugs inferred by drug-target network analysis, palbociclib, alpelisib, ribociclib, dexamethasone, checkpoint kinase inhibitor AXD 7762, irinotecan, milademetan tosylate, R05045337, cisplatin, prexasertib, and olaparib were considered promising drug candidates targeting genes involved in at least two E:C fusion-related pathways.
Affected Others Responsivity to Gambling Harm: An International Taxonomy of Consumer-Derived Behaviour Change Techniques
Authors: Booth Natalia , Dowling Nicki , Landon Jason , Lubman Dan , Merkouris Stephanie , Rodda Simone
Published: February 2021

Affected others impacted by someone else’s gambling utilise numerous behaviour change strategies to minimise gambling-related harm but knowledge on what these strategies are and how they are implemented is limited. This study aimed to develop a comprehensive data-driven taxonomy of the types of self-help strategies used by affected others, and to categorize these into high-level behaviour change techniques (BCTs). Two taxonomies were developed using an inductive and deductive approach which was applied to a dataset of online sources and organised into the Rubicon model of action phases. These taxonomies were family-focused (how to reduce the impact of gambling harm on families) and gambler-focused (how to support the gambler in behaviour change). In total, 329 online sources containing 3536 different strategies were identified. The family-focused classification contained 16 BCTs, and the most frequent were professional support, financial management and planned consequences. The gambler-focused classification contained 11 BCTs, and the most frequent were feedback on behaviours, professional support and financial management. The majority of family- and gambler-focused BCTs fell under the actional phase of the Rubicon model. Grounded in lived experience, the findings highlight the need for intervention and resource development that includes a wide range of specific techniques that affected others can utilise.
Six-Minute Walking Test Performance Relates to Neurocognitive Abilities in Preschoolers
Authors: Keye Shelby A. , Walk Anne M. , Cannavale Corinne N. , Iwinski Samantha , McLoughlin Gabriella M. , Steinberg Linda G. , Khan Naiman A.
Published: February 2021

This study investigated the relationship between six-minute walking test (6MWT) distance walked and preschool-aged children’s academic abilities, and behavioral and event-related potentials (ERP) indices of cognitive control. There were 59 children (25 females; age: 5.0 ± 0.6 years) who completed a 6MWT (mean distance: 449.6 ± 82.0 m) to estimate cardiorespiratory fitness. The Woodcock Johnson Early Cognitive and Academic Development Test evaluated academic abilities. A modified Eriksen flanker, hearts and flowers task, and auditory oddball task eliciting ERPs (N2, P3) assessed cognitive control. After adjusting for adiposity, diet, and demographics, linear regressions resulted in positive relationships between 6MWT distance and General Intellectual Ability (β = 0.25, Adj R2 = 0.04, p = 0.04) and Expressive Language (β = 0.30, Adj R2 = 0.13, p = 0.02). 6MWT distance was positively correlated with congruent accuracy (β = 0.29, Adj R2 = 0.18, p < 0.01) and negatively with incongruent reaction time (β = −0.26, Adj R2 = 0.05, p = 0.04) during the flanker task, and positively with homogeneous (β = 0.23, Adj R2 = 0.21, p = 0.04) and heterogeneous (β = 0.26, Adj R2 = 0.40, p = 0.02) accuracy on the hearts and flowers task. Higher fit children showed faster N2 latencies and greater P3 amplitudes to target stimuli; however, these were at the trend level following the adjustment of covariates. These findings indicate that the positive influence of cardiorespiratory fitness on cognitive function is evident in 4–6-year-olds.
Corneal Penetration of Low-Dose Atropine Eye Drops
Authors: Austermann Henning , Schaeffel Frank , Mathis Ute , Hund Verena , Mußhoff Frank , Ziemssen Focke , Schnichels Sven
Published: February 2021

Major studies demonstrating the inhibition of myopia in children and juveniles by low-dose atropine eye drops provide little information on the manufacturing process and the exact composition of the atropine dilutions. However, corneal penetration might significantly vary depending on preservatives, such as benzalkonium chloride (BAC), and the atropine concentration. Since there is a trade-off between side effects, stability, and optimal effects of atropine on myopia, it is important to gain better knowledge about intraocular atropine concentrations. We performed an ex vivo study to determine corneal penetration for different formulations. Atropine drops (0.01%) of different formulations were obtained from pharmacies and applied to the cornea of freshly enucleated pig eyes. After 10 min, a sample of aqueous humor was taken and atropine concentrations were determined after liquid–liquid extraction followed by high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS). The variability that originated from variations in applied drop size exceeded the differences between preserved and preservative-free formulations. The atropine concentration in the anterior chamber measured after 10 min was only 3.8 × 10−8 of its concentration in the applied eye drops, corresponding to 502.4 pM. Obviously, the preservative did not facilitate corneal penetration, at least ex vivo. In the aqueous humor of children’s eyes, similar concentrations, including higher variability, may be expected in the lower therapeutic window of pharmacodynamic action.
Sex, Type of Surgery, and Surgical Site Infections Are Associated with Perioperative Cortisol in Colorectal Cancer Patients
Authors: Fleszar Mariusz G. , Fortuna Paulina , Zawadzki Marek , Hodurek Paweł , Bednarz-Misa Iwona , Witkiewicz Wojciech , Krzystek-Korpacka Małgorzata
Published: February 2021

Excessive endocrine response to trauma negatively affects patients’ well-being. Cortisol dynamics following robot-assisted colorectal surgery are unknown. We aimed at determining the impact of cancer pathology and surgery-related factors on baseline cortisol levels and analyzed its time-profile in colorectal cancer patients undergoing open or robot-assisted surgery. Cortisol levels were measured using liquid chromatography quadrupole time-of-flight mass spectrometry. Baseline cortisol was not associated with any patient- or disease-related factors. Post-surgery cortisol increased by 36% at 8 h and returned to baseline on postoperative day three. The cortisol time profile was significantly affected by surgery type, estimated blood loss, and length of surgery. Baseline-adjusted cortisol increase was greater in females at hour 8 and in both females and patients from open surgery group at hour 24. Solely in the open surgery group, cortisol dynamics paralleled changes in interleukin (IL)-1β, IL-10, IL-1ra, IL-7, IL-8 and tumor necrosis factor (TNF)-α but did not correlate with changes in IL-6 or interferon (IFN)-γ at any time-point. Cortisol co-examined with C-reactive protein was predictive of surgical site infections (SSI) with high accuracy. In conclusion, patient’s sex and surgery invasiveness affect cortisol dynamics. Surgery-induced elevation can be reduced by minimally invasive robot-assisted procedures. Cortisol and C-reactive protein as SSI biomarkers might be of value in the evaluation of safety of early discharge of patients.
Leukocyte Telomere Length Is Not Reduced in Children and Adults with Cystic Fibrosis but Associates with Clinical Characteristics—A Cross-Sectional Study
Authors: Glapa-Nowak Aleksandra , Mutt Shivaprakash Jagalur , Lisowska Aleksandra , Sapiejka Ewa , Goździk-Spychalska Joanna , Wieczorek-Filipiak Mirosława , Drzymała-Czyż Sławomira , Nowak Jan Krzysztof , Thalmann Olaf , Herzig Karl-Heinz , Walkowiak Jarosław
Published: February 2021

We hypothezied that telomere length is considerably altered in cystic fibrosis (CF) patients compared to healthy subjects (HS), and that leukocyte telomere length variation reflects the severity of CF. Relative telomere length (RTL) was assessed by qPCR in 70 children aged 5–10 (34 CF; 36 HS) and 114 adults aged 18–45 (53 CF; 61 HS). Telomere length was similar in CF and HS (median (interquartile range): 0.799 (0.686–0.950) vs. 0.831 (0.707–0.986); p = 0.5283) both in children and adults. In adults, women had longer telomeres than men (0.805 (0.715–0.931) vs. 0.703 (0.574–0.790); p = 0.0002). Patients treated with inhaled corticosteroids had a shorter RTL compared to those without steroid therapy (0.765 (0.664–0.910) vs. 0.943 (0.813–1.191); p = 0.0007) and this finding remained significant after adjusting for gender, age, BMI, and child/adult status (p = 0.0003). Shorter telomeres were independently associated with the presence of comorbidities (0.763 (0.643–0.905) vs. 0.950 (0.783–1.130); p = 0.0006) and antibiotic treatment at the moment of blood sampling (0.762 (0.648–0.908) vs. 0.832 (0.748–1.129); p = 0.0172). RTL correlated with number of multiple-day hospitalizations (rho = −0.251; p = 0.0239), as well as number of hospitalization days (rho = −0.279; p = 0.0113). Leukocyte RTL in children and adults with CF was not shorter than in healthy controls, and did not seem to have any potential as a predictor of CF survival. However, it inversely associated with the investigated clinical characteristics.
Optimal Allocation of the Limited COVID-19 Vaccine Supply in South Korea
Authors: Shim Eunha
Published: February 2021

Initial supply of the coronavirus disease (COVID-19) vaccine may be limited, necessitating its effective use. Herein, an age-structured model of COVID-19 spread in South Korea is parameterized to understand the epidemiological characteristics of COVID-19. The model determines optimal vaccine allocation for minimizing infections, deaths, and years of life lost while accounting for population factors, such as country-specific age distribution and contact structure, and various levels of vaccine efficacy. A transmission-blocking vaccine should be prioritized in adults aged 20–49 years and those older than 50 years to minimize the cumulative incidence and mortality, respectively. A strategy to minimize years of life lost involves the vaccination of adults aged 40–69 years, reflecting the relatively high case-fatality rates and years of life lost in this age group. An incidence-minimizing vaccination strategy is highly sensitive to vaccine efficacy, and vaccines with lower efficacy should be administered to teenagers and adults aged 50–59 years. Consideration of age-specific contact rates and vaccine efficacy is critical to optimize vaccine allocation. New recommendations for COVID-19 vaccines under consideration by the Korean Centers for Disease Control and Prevention are mainly based on a mortality-minimizing allocation strategy.
Flapless Surgical Approach to Extract Impacted Inferior Third Molars: A Retrospective Clinical Study
Authors: Materni Alberto , De Angelis Nicola , Di Tullio Nicolò , Colombo Esteban , Benedicenti Stefano , Amaroli Andrea
Published: February 2021

This study aimed to compare a flapless surgical approach (FSA) with a traditional envelope flap (traditional approach (TA)). Every patient was treated with two approaches: TA and FSA. The primary outcome variables were both the discomfort during the post-operative convalescence and the correct final recovery of the impacted area. The secondary outcome variable was the average duration of the surgery. Post-operative pain and oedema were recorded. The measurements of soft tissue interface toward the distobuccal edge of the second molar were taken by periodontal probe before surgery (baseline) and 8 weeks after surgery. Statistical software was used to evaluate the data; a p-value < 0.05 was considered statistically significant. Twenty-four teeth of 12 patients (six Caucasian males and six Caucasian females, aged 23 ± 4 (17–30) years) with both lower impacted third molars (Ms3) were analysed. Considering an alpha error 0.05 that sample size allows power from 0.80 to 0.90, depending on the variable evaluated. Concerning attached gingiva, oedema and pain, the linear mixed model resulted in a statistically significant difference between the TA and FSA (p = 0.003; p < 0.01; and p = 0.018, respectively). Conversely, the model did not show a difference (p = 0.322) if pocket probing depth was considered. The FSA procedure was faster (p < 0.05) than the TA procedure (17 min and 8 s (±6 s) vs. 28 min and 6 s (±4 s), respectively). The results suggest that the FSA could be a suitable option for improving the surgical removal of lower Ms3. However, additional randomized controlled trial studies are necessary to confirm the reliability of our procedure and to verify its suitability in more complex Ms3 classifications.
Pseudothrombocytopenia—A Review on Causes, Occurrence and Clinical Implications
Authors: Lardinois Benjamin , Favresse Julien , Chatelain Bernard , Lippi Giuseppe , Mullier François
Published: February 2021

Pseudothrombocytopenia (PTCP), a relative common finding in clinical laboratories, can lead to diagnostic errors, overtreatment, and further (even invasive) unnecessary testing. Clinical consequences with potential life-threatening events (e.g., unnecessary platelet transfusion, inappropriate treatment including splenectomy or corticosteroids) are still observed when PTCP is not readily detected. The phenomenon is even more complex when occurring with different anticoagulants. In this review we present a case of multi-anticoagulant PTCP, where we studied different parameters including temperature, amikacin supplementation, measurement methods, and type of anticoagulant. Prevalence, clinical risk factors, pre-analytical and analytical factors, along with clinical implications, will be discussed. The detection of an anticoagulant-dependent PTCP does not necessarily imply the presence of specific disorders. Conversely, the incidence of PTCP seems higher in patients receiving low molecular weight heparin, during hospitalization, or in men aged 50 years or older. New analytical technologies, such as fluorescence or optical platelet counting, will be soon overturning traditional algorithms and represent valuable diagnostic aids. A practical laboratory approach, based on current knowledge of PTCP, is finally proposed for overcoming spuriously low platelet counts.
Genome-Wide Binding Analyses of HOXB1 Revealed a Novel DNA Binding Motif Associated with Gene Repression
Authors: Singh Narendra Pratap , De Kumar Bony , Paulson Ariel , Parrish Mark E. , Scott Carrie , Zhang Ying , Florens Laurence , Krumlauf Robb
Published: February 2021

Knowledge of the diverse DNA binding specificities of transcription factors is important for understanding their specific regulatory functions in animal development and evolution. We have examined the genome-wide binding properties of the mouse HOXB1 protein in embryonic stem cells differentiated into neural fates. Unexpectedly, only a small number of HOXB1 bound regions (7%) correlate with binding of the known HOX cofactors PBX and MEIS. In contrast, 22% of the HOXB1 binding peaks display co-occupancy with the transcriptional repressor REST. Analyses revealed that co-binding of HOXB1 with PBX correlates with active histone marks and high levels of expression, while co-occupancy with REST correlates with repressive histone marks and repression of the target genes. Analysis of HOXB1 bound regions uncovered enrichment of a novel 15 base pair HOXB1 binding motif HB1RE (HOXB1 response element). In vitro template binding assays showed that HOXB1, PBX1, and MEIS can bind to this motif. In vivo, this motif is sufficient for direct expression of a reporter gene and over-expression of HOXB1 selectively represses this activity. Our analyses suggest that HOXB1 has evolved an association with REST in gene regulation and the novel HB1RE motif contributes to HOXB1 function in part through a repressive role in gene expression.
The Ankle Joint Range of Motion and Its Effect on Squat Jump Performance with and without Arm Swing in Adolescent Female Volleyball Players
Authors: Panoutsakopoulos Vassilios , Kotzamanidou Mariana C. , Papaiakovou Georgios , Kollias Iraklis A.
Published: February 2021

A flexible ankle joint is suggested to be a contributing factor for vertical squat jump (SQJ) performance. The purpose of the study was to investigate the effect of the active (ACT) and passive (PAS) ankle joint range of motion (ROM) on SQJ performed by adolescent female volleyball players. ACT and PAS ankle ROM at knee extension angles of 90, 140, and 180 degrees (180 degrees: full extension) were measured with a video analysis method for 35 female post-pubertal volleyball players (16.3 ± 1.1 yrs, 1.80 ± 0.04 m, 68.8 ± 6.8 kg). Additionally, the players fulfilling previously recommended criteria were assigned to the flexible (n = 10) and inflexible (n = 8) groups and executed SQJ with and without an arm swing on a force-plate. Results of the 2 × 2 × 3 MANOVA revealed a significant (p < 0.05) flexibility type and knee angle effect, as ankle ROM was larger in PAS compared to ACT and as the knee joint progressed from 90 to 180 degrees extension. The 2 × 2 ANOVA revealed a significant (p < 0.05) group effect, as flexible players jumped higher in the arm swing SQJ, along with a significant arm swing effect on key SQJ kinetic parameters. In conclusion, a more flexible ankle joint result in improved SQJ performance. Therefore, ankle flexibility training should be implemented in youth volleyball players.
Real-Time Quality Control of Heat Sealed Bottles Using Thermal Images and Artificial Neural Network
Authors: Cruz Samuel , Paulino António , Duraes Joao , Mendes Mateus
Published: February 2021

Quality control of heat sealed bottles is very important to minimize waste and in some cases protect people’s health. The present paper describes a case study where an automated non invasive and non destructive quality control system was designed to assess the quality of the seals of bottles containing pesticide. In this case study, the integrity of the seals is evaluated using an artificial neural network based on images of the seals processed with computer vision techniques. Because the seals are not directly visible from the bottle exterior, the images are infrared pictures obtained using a thermal camera. The method is non invasive, automated, and can be applied to common conveyor belts currently used in industrial plants. The results show that the inspection process is effective in identifying defective seals with a precision of 98.6% and a recall of 100% and because it is automated it can be scaled up to large bottle processing plants.
Morphology and Phylogeny of Scrippsiella precaria Montresor & Zingone (Thoracosphaerales, Dinophyceae) from Korean Coastal Waters
Authors: Kim Hyun Jung , Li Zhun , Kang Nam Seon , Gu Haifeng , Kim Daekyung , Seo Min Ho , Lee Sang Deuk , Yun Suk Min , Oh Seok-Jin , Shin Hyeon Ho
Published: February 2021

The dinoflagellate genus Scrippsiella is a common member of phytoplankton and their cysts are also frequently reported in coastal sediments worldwide. However, the diversity of Scrippsiella in Korean waters has not been fully investigated. Here, several isolates of Scrippsiella precaria collected from Korean waters and germinated from resting cysts were examined using light and scanning electron microscopy. The resting cysts were characterized by pointed calcareous spines and one or two red accumulation bodies, and the archeopyle was mesoepicystal, representing the loss of 2–4′ and 1–3a paraplates. Rounded resting cysts were found in culture, and an increase in spine length was observed until 8 days of development. Korean isolates of S. precaria had the plate formula of Po, X, 4′, 3a, 7″, 6C, 4S, 5‴, 2⁗. There were differences in the cell size and location of the red body between Korean isolates and previously described cells of S. precaria. In addition, the Korean isolates of S. precaria had two types of the 5″ plate that either contacted the 2a plate or not. Molecular phylogeny based on internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequences revealed that the Korean isolates were nested within the subclade of PRE (S. precaria and related species) in the clade of Scrippsiella sensu lato, and that the PRE subclade had two ribotypes: ribotype 1 consisting of the isolates from Korea, China, and Australia, and ribotype 2 consisting of the isolates from Italy and Greece. Lineages between isolates of ribotype 1 were likely to be related to the dispersal by ocean currents and ballast waters from international shipping, and the two types of spine shapes and locations of the 5″ plates may be a distinct feature for ribotype 1.
Bioremediation of Diesel Contaminated Marine Water by Bacteria: A Review and Bibliometric Analysis
Authors: Khalid Farah Eryssa , Lim Zheng Syuen , Sabri Suriana , Gomez-Fuentes Claudio , Zulkharnain Azham , Ahmad Siti Aqlima
Published: February 2021

Oil pollution can cause tremendous harm and risk to the water ecosystem and organisms due to the relatively recalcitrant hydrocarbon compounds. The current chemical method used to treat the ecosystem polluted with diesel is incompetent and expensive for a large-scale treatment. Thus, bioremediation technique seems urgent and requires more attention to solve the existing environmental problems. Biological agents, including microorganisms, carry out the biodegradation process where organic pollutants are mineralized into water, carbon dioxide, and less toxic compounds. Hydrocarbon-degrading bacteria are ubiquitous in the nature and often exploited for their specialty to bioremediate the oil-polluted area. The capability of these bacteria to utilize hydrocarbon compounds as a carbon source is the main reason behind their species exploitation. Recently, microbial remediation by halophilic bacteria has received many positive feedbacks as an efficient pollutant degrader. These halophilic bacteria are also considered as suitable candidates for bioremediation in hypersaline environments. However, only a few microbial species have been isolated with limited available information on the biodegradation of organic pollutants by halophilic bacteria. The fundamental aspect for successful bioremediation includes selecting appropriate microbes with a high capability of pollutant degradation. Therefore, high salinity bacteria are remarkable microbes for diesel degradation. This paper provides an updated overview of diesel hydrocarbon degradation, the effects of oil spills on the environment and living organisms, and the potential role of high salinity bacteria to decontaminate the organic pollutants in the water environment.
Halophytophthora fluviatilis Pathogenicity and Distribution along a Mediterranean-Subalpine Gradient
Authors: Caballol Maria , Štraus Dora , Macia Héctor , Ramis Xavier , Redondo Miguel Á. , Oliva Jonàs
Published: February 2021

Halophytophthora species have been traditionally regarded as brackish water oomycetes; however, recent reports in inland freshwater call for a better understanding of their ecology and possible pathogenicity. We studied the distribution of Halophytophthora fluviatilis in 117 forest streams by metabarcoding river filtrates taken in spring and autumn and by direct isolation from floating leaves. Pathogenicity on six Fagaceae species and Alnus glutinosa was assessed by stem inoculations. The distribution of H. fluviatilis was correlated with high mean annual temperatures (>93.5% of reports in Ta > 12.2 °C) and low precipitation records. H. fluviatilis was therefore widely distributed in forest streams in a warm–dry climate, but it was mostly absent in subalpine streams. H. fluviatilis was primarily detected in autumn with few findings in spring (28.4% vs. 2.7% of streams). H. fluviatilis was able to cause small lesions on some tree species such as Quercus pubescens, Q. suber and A. glutinosa. Our findings suggest that H. fluviatilis may be adapted to warm and dry conditions, and that it does not pose a significant threat to the most common Mediterranean broadleaved trees.
Techniques for the Assessment of In Vitro and In Vivo Antifungal Combinations
Authors: Bidaud Anne-Laure , Schwarz Patrick , Herbreteau Guillaume , Dannaoui Eric
Published: February 2021

Systemic fungal infections are associated with high mortality rates despite adequate treatment. Moreover, acquired resistance to antifungals is increasing, which further complicates the therapeutic management. One strategy to overcome antifungal resistance is to use antifungal combinations. In vitro, several techniques are used to assess drug interactions, such as the broth microdilution checkerboard, agar-diffusion methods, and time-kill curves. Currently, the most widely used technique is the checkerboard method. The aim of all these techniques is to determine if the interaction between antifungal agents is synergistic, indifferent, or antagonistic. However, the interpretation of the results remains difficult. Several methods of analysis can be used, based on different theories. The most commonly used method is the calculation of the fractional inhibitory concentration index. Determination of the usefulness of combination treatments in patients needs well-conducted clinical trials, which are difficult. It is therefore important to study antifungal combinations in vivo, in experimental animal models of fungal infections. Although mammalian models have mostly been used, new alternative animal models in invertebrates look promising. To evaluate the antifungal efficacy, the most commonly used criteria are the mortality rate and the fungal load in the target organs.
Updates in Paracoccidioides Biology and Genetic Advances in Fungus Manipulation
Authors: Chaves Alison Felipe Alencar , Navarro Marina Valente , de Barros Yasmin Nascimento , Silva Rafael Souza , Xander Patricia , Batista Wagner Luiz
Published: February 2021

The dimorphic fungi of the Paracoccidioides genus are the causative agents of paracoccidioidomycosis (PCM). This disease is endemic in Latin America and primarily affects workers in rural areas. PCM is considered a neglected disease, despite being a disabling disease that has a notable impact on the public health system. Paracoccidioides spp. are thermally dimorphic fungi that present infective mycelia at 25 °C and differentiate into pathogenic yeast forms at 37 °C. This transition involves a series of morphological, structural, and metabolic changes which are essential for their survival inside hosts. As a pathogen, the fungus is subjected to several varieties of stress conditions, including the host immune response, which involves the production of reactive nitrogen and oxygen species, thermal stress due to temperature changes during the transition, pH alterations within phagolysosomes, and hypoxia inside granulomas. Over the years, studies focusing on understanding the establishment and development of PCM have been conducted with several limitations due to the low effectiveness of strategies for the genetic manipulation of Paracoccidioides spp. This review describes the most relevant biological features of Paracoccidioides spp., including aspects of the phylogeny, ecology, stress response, infection, and evasion mechanisms of the fungus. We also discuss the genetic aspects and difficulties of fungal manipulation, and, finally, describe the advances in molecular biology that may be employed in molecular research on this fungus in the future.
Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report
Authors: Sterczała Barbara , Grzech-Leśniak Kinga , Michel Olga , Trzeciakowski Witold , Dominiak Marzena , Jurczyszyn Kamil
Published: February 2021

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types, Nd:YAG (1064 nm), infrared diode laser (980 nm), and prototype led laser emitting 405, 450, and 635 nm were used to irradiate the fibroblasts. Due to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm2, 25 J/cm2, 64 J/cm2. The viability and proliferation of cells were determined using the (Thiazolyl Blue Tetrazolium Blue) (MTT) test conducted 24, 48, and 72 h after laser irradiation. Results: The highest percentage of mitochondrial activity (MA = 122.1%) was observed in the group irradiated with the 635 nm laser, with an energy density of 64 J/cm2 after 48 h. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405 nm laser at 25 J/cm2 gave similar results to the 635 nm laser. Conclusions: The application of the 635 nm and 405 nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.
Image-Guided Localization Techniques for Surgical Excision of Non-Palpable Breast Lesions: An Overview of Current Literature and Our Experience with Preoperative Skin Tattoo
Authors: Franceschini Gianluca , Mason Elena Jane , Grippo Cristina , D’Archi Sabatino , D’Angelo Anna , Scardina Lorenzo , Sanchez Alejandro Martin , Conti Marco , Trombadori Charlotte , Terribile Daniela Andreina , Di Leone Alba , Carnassale Beatrice , Belli Paolo , Manfredi Riccardo , Masetti Riccardo
Published: February 2021

Breast conserving surgery has become the standard of care and is more commonly performed than mastectomy for early stage breast cancer, with recent studies showing equivalent survival and lower morbidity. Accurate preoperative lesion localization is mandatory to obtain adequate oncological and cosmetic results. Image guidance assures the precision requested for this purpose. This review provides a summary of all techniques currently available, ranging from the classic wire positioning to the newer magnetic seed localization. We describe the procedures and equipment necessary for each method, outlining the advantages and disadvantages, with a focus on the cost-effective preoperative skin tattoo technique performed at our centre. Breast surgeons and radiologists have to consider ongoing technological developments in order to assess the best localization method for each individual patient and clinical setting.
Fecal Microbiota Transplantation in Allogeneic Hematopoietic Stem Cell Transplantation Recipients: A Systematic Review
Authors: Pession Andrea , Zama Daniele , Muratore Edoardo , Leardini Davide , Gori Davide , Guaraldi Federica , Prete Arcangelo , Turroni Silvia , Brigidi Patrizia , Masetti Riccardo
Published: February 2021

The disruption of gut microbiota eubiosis has been linked to major complications in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. Various strategies have been developed to reduce dysbiosis and related complications. Fecal microbiota transplantation (FMT) consists of the infusion of fecal matter from a healthy donor to restore impaired intestinal homeostasis, and could be applied in the allo-HSCT setting. We conducted a systematic review of studies addressing the use of FMT in allo-HSCT patients. In the 23 papers included in the qualitative synthesis, FMT was used for the treatment of recurrent Clostridioides difficile infections or as a therapeutic strategy for steroid-resistant gut aGvHD. FMT was also performed with a preventive aim (e.g., to decolonize from antibiotic-resistant bacteria). Additional knowledge on the biological mechanisms underlying clinical findings is needed in order to employ FMT in clinical practice. There is also concern regarding the administration of microbial consortia in immune-compromised patients with altered gut permeability. Therefore, the safety profile and efficacy of the procedure must be determined to better assess the role of FMT in allo-HSCT recipients.
Salivary Micro-RNA and Oral Squamous Cell Carcinoma: A Systematic Review
Authors: Menini Maria , De Giovanni Emanuele , Bagnasco Francesco , Delucchi Francesca , Pera Francesco , Baldi Domenico , Pesce Paolo
Published: February 2021

Oral squamous cell carcinoma (OSCC) is a widespread malignancy with high mortality. In particular, a delay in its diagnosis dramatically decreases the survival rate. The aim of this systematic review was to investigate and summarize clinical results in the literature, regarding the potential use of salivary microRNAs (miRNAs) as diagnostic and prognostic biomarkers for OSCC patients. Twelve papers were selected, including both case–control and cohort studies, and all of them detected significantly dysregulated miRNAs in OSCC patients compared to healthy controls. Based on our results, salivary miRNAs might provide a non-invasive and cost-effective method in the diagnosis of OSCC, and also to monitor more easily its evolution and therapeutic response and therefore aid in the establishment of specific therapeutic strategies.
Small and Medium-Sized Enterprises, Business Demography and European Socio-Economic Model: Does the Paradigm Really Converge?
Authors: Andrei Jean Vasile , Chivu Luminita , Gheorghe Ileana Georgiana , Grubor Aleksandar , Sedlarski Teodor , Sima Violeta , Subić Jonel , Vasic Mile
Published: February 2021

Although the European business environment induces important premises and assures conditions in determining economic growth and social well-being, the determinant and existent connections between the evolution of small and medium-sized enterprises (SMEs), business demography characteristics and the European socio-economic model have been scarcely studied in recent years. The dimensions of the European socio-economic model design a very specific framework in developing business demography and assuring a favorable environment for future SME development. The main aim of the manuscript is to investigate the evolution of the European SMEs sector and the perspective of business demography evolution to converge with exigencies of the European socio-economic model. In order to argue the research objective, eight specific and representative business demography variables were employed, from 12 European Union member states (EU-MS), during 2009–2017. Further, the SMEs’ performances, determined by changing the economic functional paradigm, were assessed. For proving this, an econometric model was designed considering labor productivity as an endogenous variable. Our preliminary analysis shows considerable differences in business demography indicators and SMEs development among all five socio-economic sub-models of the main European socio-economic model, proving a tight connection between European socio-economic models and SMEs’ performance and arguing the necessity of a paradigm convergence. Within some sub-models, there is clear evidence of clustering and convergence in terms of business demography and SMEs future development.
Soil Protection in Floodplains—A Review
Authors: El Hourani Mariam , Broll Gabriele
Published: February 2021

Soils in floodplains and riparian zones provide important ecosystem functions and services. These ecosystems belong to the most threatened ecosystems worldwide. Therefore, the management of floodplains has changed from river control to the restoration of rivers and floodplains. However, restoration activities can also negatively impact soils in these areas. Thus, a detailed knowledge of the soils is needed to prevent detrimental soil changes. The aim of this review is therefore to assess the kind and extent of soil information used in research on floodplains and riparian zones. This article is based on a quantitative literature search. Soil information of 100 research articles was collected. Soil properties were divided into physical, chemical, biological, and detailed soil classification. Some kind of soil information like classification is used in 97 articles, but often there is no complete description of the soils and only single parameters are described. Physical soil properties are mentioned in 76 articles, chemical soil properties in 56 articles, biological soil properties in 21 articles, and a detailed soil classification is provided in 32 articles. It is recommended to integrate at least a minimum data set on soil information in all research conducted in floodplains and riparian zones. This minimum data set comprises soil types, coarse fragments, texture and structure of the soil, bulk density, pH, soil organic matter, water content, rooting depth, and calcium carbonate content. Additionally, the nutrient and/or pollution status might be a useful parameter.
Three Decades of Land Cover Change in East Africa
Authors: Bullock Eric L. , Healey Sean P. , Yang Zhiqiang , Oduor Phoebe , Gorelick Noel , Omondi Steve , Ouko Edward , Cohen Warren B.
Published: February 2021

Population growth rates in Sub-Saharan East Africa are among the highest in the world, creating increasing pressure for land cover conversion. To date, however, there has been no comprehensive assessment of regional land cover change, and most long-term trends have not yet been quantified. Using a designed sample of satellite-based observations of historical land cover change, we estimate the areas and trends in nine land cover classes from 1998 to 2017 in Ethiopia, Kenya, Uganda, Malawi, Rwanda, Tanzania, and Zambia. Our analysis found an 18,154,000 (±1,580,000) ha, or 34.8%, increase in the area of cropland in East Africa. Conversion occurred primarily from Open Grasslands, Wooded Grasslands, and Open Forests, causing a large-scale reduction in woody vegetation classes. We observed far more conversion (by approximately 20 million hectares) of woody classes to less-woody classes than succession in the direction of increasing trees and shrubs. Spatial patterns within our sample highlight regional land cover conversion hotspots, such as the Central Zambezian Miombo Woodlands, as potential areas of concern related to the conservation of natural ecosystems. Our findings reflect a rapidly growing population that is moving into new areas, with a 43.5% increase in the area of Settlements over the three-decade period. Our results show the areas and ecoregions most impacted by three decades of human development, both spatially and statistically.
Assessing Tradeoffs between Development and Conservation: A Case of Land Use Change in a National Park of Korea
Authors: Kim Miju , Lee Sangkwon , Oh Chi-Ok
Published: February 2021

Protected areas are places that provide diverse ecosystem services, including cultural ecosystem services. At the same time, the development and unbalanced use of natural resources in protected areas often create environmental threats and social conflicts. This study estimates the economic value of environmental consequences derived from the construction of an airport in a national park in Korea. We employ a discrete choice experiment to assess economic values and consider a key part of the tradeoffs derived from the airport construction at Heuksan Island of Dadohae Marine National Park in Korea. The results show that social benefits related to improved accessibility and tourism opportunities would be generated but at substantial costs resulting from environmental degradation and the reduction in bird populations. A segmentation analysis also reveals that heterogeneous preferences exist based on variables reflecting individuals’ visit experiences and trust in the government. The findings indicate that considering environmental impacts is important when evaluating the feasibility of a development project in a protected area.
Bird Diversity Unconsciously Increases People’s Satisfaction with Where They Live
Authors: Hepburn Lauren , Smith Adam C. , Zelenski John , Fahrig Lenore
Published: February 2021

There is growing evidence that exposure to nature increases human well-being, including in urban areas. However, relatively few studies have linked subjective satisfaction to objective features of the environment. In this study we explore the links among objective environmental features (tree cover, water, and bird diversity) and subjective judgements of satisfaction. We surveyed residents of Ottawa, Canada (n = 1035) about their satisfaction with their local neighbourhoods. We then compared the survey responses to measures of nature near their homes, including bird diversity (number of bird species), tree canopy cover, and distance to water. After controlling for effects of income and subjective happiness, residents’ neighbourhood satisfaction was positively related to the number of bird species nearby, even before participants were prompted to consider nature. Residents’ appreciation of their local neigbourhood relative to others also increased with tree canopy cover and nearness to water. Unsolicited comments from participants following the survey indicated that while residents consciously appreciate trees and water, the relationship between bird diversity and neighbourhood satisfaction appears to be unconscious; very few of the participants mentioned birds. Based on these results, we speculate that a diverse local bird community may provoke feelings of satisfaction through their presence, activity, and songs. Our results create a compelling argument for city planners and individual residents to maintain bird-friendly spaces in urban areas.
Ecosystem Service Use and the Motivations for Use in Central Parks in Three European Cities
Authors: Priess Jörg , Pinto Luis Valença , Misiune Ieva , Palliwoda Julia
Published: February 2021

The majority of Europeans live in cities, where parks as components of Urban Green Spaces (UGSs) play an important role in well-being and the provision of ecosystem services (ES). UGSs are especially relevant for the implementation of the United Nations (UN) Agenda 2030 Sustainable Development Goals “Good health and wellbeing” (Goal 3) and “Sustainable cities and communities” (Goal 11). This study focused on ES use and users’ motives, which were surveyed during visits at central parks in the cities Leipzig, Coimbra and Vilnius. Park visitors used 17 different ES, dominated by physical interactions such as walking or biking, followed by experiential and aesthetical ES and ES linked to social relations. Age of visitors, cultural setting and distance to homes influenced ES use in the parks differently in each city, limiting the transferability of park—user behaviour or motivations across different spatial and cultural contexts. Results also indicate that aligning sustainability objectives and usability, good accessibility of urban parks plays a central role and encourages the use of non-motorized or public transport for park visits. Concrete information about UGS user motivation and behaviour generated in this and similar studies contributes to convert the UN Agenda 2030 strategies at the municipal level into sustainability and user-oriented design and management of UGS.
Investment in Land Restoration: New Perspectives with Special Reference to Australia
Authors: Leake John E.
Published: February 2021

Environmental services of biodiversity, clean water, etc., have been considered byproducts of farming and grazing, but population pressures and a move from rural to peri-urban areas are changing land use practices, reducing these services and increasing land degradation. A range of ecosystem markets have been reversing this damage, but these are not widely institutionalized, so land managers do not see them as “real” in the way they do for traditional food and fiber products. There are difficulties defining and monitoring non-food/fiber ecosystem services so they can be reliably marketed, and those markets that do operate usually do so in a piecemeal single product way in the interest of simplicity for the buyer, and seldom adequately regulate or compensate land managers for non-market benefits. New profitable uses of degraded water and regenerating land are emerging, but they require technology transfer or supply chain development to facilitate adoption. There is a need for a transformational change in the way land and water are used to promote a broader approach, so environmental services become a mainstream activity for land managers. A far-sighted Philanthropist is required to support an International institution to take up the challenge of institutionalizing such a ‘brokerage’ system to operate globally.