bluuebunny/crossref_metadata_2025 huggingface dataset:
Alkali-Activation of Synthetic Aluminosilicate Glass With Basaltic Composition
Authors: Alzeer Mohammad I. M. , Nguyen Hoang , Cheeseman Christopher , Kinnunen Paivo
Published: September 2021

Alkali-activated materials (AAMs) are a potential alternative to Portland cement because they can have high strength, good durability and low environmental impact. This paper reports on the structural and mechanical characteristics of aluminosilicate glass with basalt-like compositions, as a feedstock for AAMs. The alkali-activation kinetics, microstructure, and mechanical performance of the alkali activated glass were investigated. The results show that AAMs prepared from basalt glass have high compressive strength (reaching up to 90 MPa after 7 days of hydration) compared to those made using granulated blast furnace slag (GBFS). In addition, calorimetry data show that the hydrolysis of the developed glass and subsequent polymerization of the reaction product occur at a faster rate compared to GBFS. Furthermore, the obtained results show that the alkali activation of the developed glass formed sodium aluminosilicate hydrate (N-A-S-H) intermixed with Ca aluminosilicate hydrate gel (C-A-S-H), while the alkali activation of GBFS resulted in predominantly C-A-S-H gel. The developed glass can be formed from carbonate-free and abundant natural resources such as basalt rocks or mixtures of silicate minerals. Therefore, the glass reported herein has high potential as a new feedstock of AAMs.
The Interaction Between the Microbiome and Tumors
Authors: Zong Yawen , Zhou Yujie , Liao Binyou , Liao Min , Shi Yangyang , Wei Yu , Huang Yuyao , Zhou Xuedong , Cheng Lei , Ren Biao
Published: September 2021

Cancer is a significant global health problem and is characterized by a consistent increase in incidence and mortality rate. Deciphering the etiology and risk factors are essential parts of cancer research. Recently, the altered microbiome has been identified within the tumor microenvironment, tumor tissue, and even nonadjacent environments, which indicates a strong correlation between the microbiome and tumor development. However, the causation and mechanisms of this correlation remain unclear. Herein, we summarized and discussed the interaction between the microbiome and tumor progression. Firstly, the microbiome, which can be located in the tumor microenvironment, inside tumor tissues and in the nonadjacent environment, is different between cancer patients and healthy individuals. Secondly, the tumor can remodel microbial profiles by creating a more beneficial condition for the shifted microbiome. Third, the microbiome can promote tumorigenesis through a direct pathogenic process, including the establishment of an inflammatory environment and its effect on host immunity. The interactions between the microbiome and tumors can promote an understanding of the carcinogenesis and provide novel therapeutic strategies for cancers.
Artemether, Artesunate, Arteannuin B, Echinatin, Licochalcone B and Andrographolide Effectively Inhibit SARS-CoV-2 and Related Viruses In Vitro
Authors: Hu Yunjia , Liu Meiqin , Qin Hongbo , Lin Haofeng , An Xiaoping , Shi Zhengli , Song Lihua , Yang Xinglou , Fan Huahao , Tong Yigang
Published: September 2021

Since the first reported case caused by the novel coronavirus SARS-CoV-2 infection in Wuhan, COVID-19 has caused serious deaths and an ongoing global pandemic, and it is still raging in more than 200 countries and regions around the world and many new variants have appeared in the process of continuous transmission. In the early stage of the epidemic prevention and control and clinical treatment, traditional Chinese medicine played a huge role in China. Here, we screened out six monomer compounds, including artemether, artesunate, arteannuin B, echinatin, licochalcone B and andrographolide, with excellent anti-SARS-CoV-2 and anti-GX_P2V activity from Anti-COVID-19 Traditional Chinese Medicine Compound Library containing 389 monomer compounds extracted from traditional Chinese medicine prescriptions “three formulas and three drugs”. Our discovery preliminary proved the stage of action of those compounds against SARS-CoV-2 and provided inspiration for further research and clinical applications.
Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion
Authors: Zou Song-Song , Zou Qing-Cui , Xiong Wen-Jing , Cui Ning-Yi , Wang Ke , Liu Hao-Xuan , Lou Wen-Juan , Higazy Doaa , Zhang Ya-Ge , Cui Min
Published: September 2021

Infection with Japanese encephalitis virus (JEV) induces high morbidity and mortality, including potentially permanent neurological sequelae. However, the mechanisms by which viruses cross the blood-brain barrier (BBB) and invade into the central nervous system (CNS) remain unclear. Here, we show that extracellular HMGB1 facilitates immune cell transmigration. Furthermore, the migration of immune cells into the CNS dramatically increases during JEV infection which may enhance viral clearance, but paradoxically expedite the onset of Japanese encephalitis (JE). In this study, brain microvascular endothelial cells (BMECs) were utilized for the detection of HMGB1 release, and leucocyte, adhesion, and the integrity of the BBB in vitro. Genetically modified JEV-expressing EGFP (EGFP-JEV) and the BBB model were established to trace JEV-infected immune cell transmigration, which mimics the process of viral neuroinfection. We find that JEV causes HMGB1 release from BMECs while increasing adhesion molecules. Recombinant HMGB1 enhances leukocyte-endothelium adhesion, facilitating JEV-infected monocyte transmigration across endothelia. Thus, JEV successfully utilizes infected monocytes to spread into the brain, expanding inside of the brain, and leading to the acceleration of JE onset, which was facilitated by HMGB1. HMGB1-promoted monocyte transmigration may represent the mechanism of JEV neuroinvasion, revealing potential therapeutic targets.
Exploring Changes in the Host Gut Microbiota During a Controlled Human Infection Model for Campylobacter jejuni
Authors: Stamps Blake W. , Kuroiwa Janelle , Isidean Sandra D. , Schilling Megan A. , Harro Clayton , Talaat Kawsar R. , Sack David A. , Tribble David R. , Maue Alexander C. , Rimmer Joanna E. , Laird Renee M. , Porter Chad K. , Goodson Michael S. , Poly Frédéric
Published: September 2021

Campylobacter jejuni infection is a leading cause of foodborne disease, common to children, adult travelers, and military populations in low- to middle-income countries. In the absence of a licensed vaccine, efforts to evaluate prophylactic agents are underway. The prophylactic efficacy of a twice-daily, 550 mg dose of the antibiotic rifaximin demonstrated no efficacy against campylobacteriosis in a controlled human infection model (CHIM); however, samples from the CHIM study were utilized to assess how the human gut microbiome responds to C. jejuni infection, and if a ‘protective’ microbiota exists in study participants not developing campylobacteriosis. Statistically significant, but minor, differences in study participant beta diversity were identified during the challenge period (p = 0.002, R2 = 0.042), but no significant differences were otherwise observed. Pre-challenge alpha diversity was elevated in study participants who did not develop campylobacteriosis compared to those who did (p < 0.001), but alpha diversity declined in all study participants from the pre-challenge period to post-discharge. Our work provides insight into gut microbiome shifts observed during a C. jejuni CHIM and following antibiotic treatment. This study utilized a high dose of 1.7 x 105 colony-forming units of C. jejuni; future work could include CHIM studies performed with inocula more closely mimicking natural exposure as well as field studies involving naturally-occurring enteric infections.
Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
Authors: Wu Jin’en , Zhang Zhihui , Teng Zhidong , Abdullah Sahibzada Waheed , Sun Shiqi , Guo Huichen
Published: September 2021

Endoplasmic reticulum (ER) stress-induced autophagy is closely associated with viral infection and propagation. However, the intrinsic link between ER stress, autophagy, and viral replication during foot-and-mouth disease virus (FMDV) infection is not fully elucidated. Our previous studies demonstrated that FMDV infection activated the ER stress-associated UPR of the PERK-eIF2a and ATF6 signaling pathway, whereas the IRE1a signaling was suppressed. We found that the activated-ATF6 pathway participated in FMDV-induced autophagy and FMDV replication, while the IRE1α pathway only affected FMDV replication. Further studies indicated that Sec62 was greatly reduced in the later stages of FMDV infection and blocked the activation of the autophagy-related IRE1α-JNK pathway. Moreover, it was also found that Sec62 promoted IRE1a phosphorylation and negatively regulated FMDV proliferation. Importantly, Sec62 may interact with LC3 to regulate ER stress and autophagy balance and eventually contribute to FMDV clearance via fusing with lysosomes. Altogether, these results suggest that Sec62 is a critical molecule in maintaining and recovering ER homeostasis by activating the IRE1α-JNK pathway and delivering autophagosome into the lysosome, thus providing new insights on FMDV-host interactions and novel antiviral therapies.
Combined Signature of the Urinary Microbiome and Metabolome in Patients With Interstitial Cystitis
Authors: Xu Hewei , Tamrat Nebiyu Elias , Gao Jie , Xu Jie , Zhou Yiduo , Zhang Sicong , Chen Zhengsen , Shao Yunpeng , Ding Liucheng , Shen Baixin , Wei Zhongqing
Published: September 2021

Interstitial cystitis (IC) is a clinical syndrome characterized by frequency, urgency, and bladder pain or pelvic pain; however, the underlying pathophysiological mechanisms and diagnostic markers are unknown. In this study, microbiome and metabolome analysis were used to explain the urine signatures of IC patients. Urine samples from 20 IC patients and 22 control groups were analyzed by using 16S rRNA sequence and liquid chromatography coupled with mass spectrometry. Four opportunistic pathogen genera, including Serratia, Brevibacterium, Porphyromonas, and Citrobacter, were significantly upregulated in IC group. The altered metabolite signatures of the metabolome may be related to sphingosine metabolism, amino acid metabolism, and fatty acid biosynthesis. Meanwhile, the associations were observed between different metabolites and microbiomes of IC. The present study suggests that the combined signatures of IC in urine microbiome and metabolome may become its prospective diagnostic markers.
Enterococcal Endocarditis: Hiding in Plain Sight
Authors: Barnes Aaron M. T. , Frank Kristi L. , Dunny Gary M.
Published: September 2021

Enterococcus faecalis is a major opportunistic bacterial pathogen of increasing clinical relevance. A substantial body of experimental evidence suggests that early biofilm formation plays a critical role in these infections, as well as in colonization and persistence in the GI tract as a commensal member of the microbiome in most terrestrial animals. Animal models of experimental endocarditis generally involve inducing mechanical valve damage by cardiac catheterization prior to infection, and it has long been presumed that endocarditis vegetation formation resulting from bacterial attachment to the endocardial endothelium requires some pre-existing tissue damage. Here we review both historical and contemporary animal model studies demonstrating the robust ability of E. faecalis to directly attach and form stable microcolony biofilms encased within a bacterially-derived extracellular matrix on the undamaged endovascular endothelial surface. We also discuss the morphological similarities when these biofilms form on other host tissues, including when E. faecalis colonizes the GI epithelium as a commensal member of the normal vertebrate microbiome - hiding in plain sight where it can serve as a source for systemic infection via translocation. We propose that these phenotypes may allow the organism to persist as an undetected infection in asymptomatic individuals and thus provide an infectious reservoir for later clinical endocarditis.
Incentivizing BECCS—A Swedish Case Study
Authors: Zetterberg Lars , Johnsson Filip , Möllersten Kenneth
Published: September 2021

Negative carbon dioxide (CO2)-emissions are prevalent in most global emissions pathways that meet the Paris temperature targets and are a critical component for reaching net-zero emissions in Year 2050. However, economic incentives supporting commercialization and deployment of BioEnergy Carbon Capture and Storage (BECCS) are missing. This Policy and Practice Review discusses five different models for creating incentives and financing for BECCS, using Sweden as an example: (1) governmental guarantees for purchasing BECCS outcomes; (2) quota obligation on selected sectors to acquire BECCS outcomes; (3) allowing BECCS credits to compensate for hard-to-abate emissions within the EU ETS; (4) private entities for voluntary compensation; and (5) other states acting as buyers of BECCS outcomes to meet their mitigation targets under the Paris Agreement. We conclude that successful implementation of BECCS is likely to require a combination of several of the Policy Models, implemented in a sequential manner. The governmental guarantee model (Model 1) is likely to be required in the shorter term, so as to establish BECCS. Policy Models 2 and 3 may become more influential over time once BECCS has been established and accepted. Model 3 links BECCS to a large carbon-pricing regime with opportunities for cost-effectiveness and expanded financing. We conclude that Policy Models 4 and 5 are associated with high levels of uncertainty regarding the timing and volume of negative emissions that can be expected—Thus, they are unlikely to trigger BECCS implementation in the short term, although may have roles in the longer term. Based on this study, we recommend that policymakers carefully consider a policy sequencing approach that is predictable and sustainable over time, for which further analyses are required. It is not obvious how such sequencing can be arranged, as the capacities to implement the different Policy Models are vested in different organizations (national governments, EU, private firms). Furthermore, it is important that a BECCS policy is part of an integrated climate policy framework, in particular one that is in line with policies aimed at the mitigation of greenhouse gas (GHG) emissions and the creation of a circular economy. It will be important to ensure that BECCS and the associated biomass resource are not overexploited. A well-designed policy package should guarantee that BECCS is neither used to postpone the reduction of fossil fuel-based emissions nor overused in the short term as a niche business for “greenwashing” while not addressing fossil fuel emissions.
Deep Learning Analysis of Echocardiographic Images to Predict Positive Genotype in Patients With Hypertrophic Cardiomyopathy
Authors: Morita Sae X. , Kusunose Kenya , Haga Akihiro , Sata Masataka , Hasegawa Kohei , Raita Yoshihiko , Reilly Muredach P. , Fifer Michael A. , Maurer Mathew S. , Shimada Yuichi J.
Published: September 2021

Genetic testing provides valuable insights into family screening strategies, diagnosis, and prognosis in patients with hypertrophic cardiomyopathy (HCM). On the other hand, genetic testing carries socio-economical and psychological burdens. It is therefore important to identify patients with HCM who are more likely to have positive genotype. However, conventional prediction models based on clinical and echocardiographic parameters offer only modest accuracy and are subject to intra- and inter-observer variability. We therefore hypothesized that deep convolutional neural network (DCNN, a type of deep learning) analysis of echocardiographic images improves the predictive accuracy of positive genotype in patients with HCM. In each case, we obtained parasternal short- and long-axis as well as apical 2-, 3-, 4-, and 5-chamber views. We employed DCNN algorithm to predict positive genotype based on the input echocardiographic images. We performed 5-fold cross-validations. We used 2 reference models—the Mayo HCM Genotype Predictor score (Mayo score) and the Toronto HCM Genotype score (Toronto score). We compared the area under the receiver-operating-characteristic curve (AUC) between a combined model using the reference model plus DCNN-derived probability and the reference model. We calculated the p-value by performing 1,000 bootstrapping. We calculated sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). In addition, we examined the net reclassification improvement. We included 99 adults with HCM who underwent genetic testing. Overall, 45 patients (45%) had positive genotype. The new model combining Mayo score and DCNN-derived probability significantly outperformed Mayo score (AUC 0.86 [95% CI 0.79–0.93] vs. 0.72 [0.61–0.82]; p < 0.001). Similarly, the new model combining Toronto score and DCNN-derived probability exhibited a higher AUC compared to Toronto score alone (AUC 0.84 [0.76–0.92] vs. 0.75 [0.65–0.85]; p = 0.03). An improvement in the sensitivity, specificity, PPV, and NPV was also achieved, along with significant net reclassification improvement. In conclusion, compared to the conventional models, our new model combining the conventional and DCNN-derived models demonstrated superior accuracy to predict positive genotype in patients with HCM.
Improved Retinal Microcirculation After Cardiac Surgery in Patients With Congenital Heart Disease
Authors: Li Cong , Zhu Zhuoting , Yuan Haiyun , Zhong Pingting , Peng Qingsheng , Dong Xinran , Huang Manqing , Liu Baoyi , Ren Yun , Kuang Yu , Zeng Xiaomin , Yu Honghua , Yang Xiaohong
Published: September 2021

Background:Microcirculatory changes in congenital heart disease (CHD) patients undergoing cardiac surgery are not fully understood. We aimed to investigate the changes of retinal microcirculation in CHD patients after cardiac surgery by optical coherence tomography angiography (OCTA) and explore the association between retinal microcirculation and surgical outcome.

Methods:This prospective observational study consisted of 71 CHD patients aged ≥6 years undergoing cardiac surgery including 19 cyanotic CHD (CCHD) and 52 acyanotic CHD (ACHD). Optical coherence tomography angiography (OCTA) was used to measure vessel density (VD) and capillary density (CD) of radial peripapillary capillary (RPC) and peripapillary, VD of superficial capillary plexus (SCP) and deep capillary plexus (DCP), thickness of retinal nerve fiber layer (RNFL) and ganglion cell complex (GCC) preoperatively and 1 month postoperatively. Transthoracic echocardiography was conducted to measure macrocirculation.

Results:In CCHD patients, VD and CD of RPC and peripapillary increased postoperatively (allP< 0.05). In ACHD patients, VD of peripapillary, CD of RPC and peripapillary, and RNFL thickness increased postoperatively (allP< 0.05). VD of SCP and DCP, and GCC thickness did not change significantly in CHD patients after surgery. Lower preoperative retinal microvascular density was associated with longer cardiopulmonary bypass (CPB) time and postoperative length of stay (PLOS). No correlation was found between microcirculatory and macrohemodynamic parameters (allP> 0.05).

Conclusions:Improved retinal microcirculation was observed after congenital cardiac surgery and impaired preoperative retinal microvasculature was associated with prolonged CPB time and PLOS, which might provide potential information about the outcome of congenital cardiac surgery.
Non-invasive Imaging in Patients With Chronic Total Occlusions of the Coronary Arteries—What Does the Interventionalist Need for Success?
Authors: Kersten Johannes , Eberhardt Nina , Prasad Vikas , Keßler Mirjam , Markovic Sinisa , Mörike Johannes , Nita Nicoleta , Stephan Tilman , Tadic Marijana , Tesfay Temsgen , Rottbauer Wolfgang , Buckert Dominik
Published: September 2021

Chronic total occlusion (CTO) of coronary arteries is a common finding in patients with known or suspected coronary artery disease (CAD). Although tremendous advances have been made in the interventional treatment of CTOs over the past decade, correct patient selection remains an important parameter for achieving optimal results. Non-invasive imaging can make a valuable contribution. Ischemia and viability, two major factors in this regard, can be displayed using echocardiography, single-photon emission tomography, positron emission tomography, computed tomography, and cardiac magnetic resonance imaging. Each has its own strengths and weaknesses. Although most have been studied in patients with CAD in general, there is an increasing number of studies with positive preselectional factors for patients with CTOs. The aim of this review is to provide a structured overview of the current state of pre-interventional imaging for CTOs.
A Critical Review on New Approaches for Chronic Disease Prevention in Brazil and Canada: From Wholistic Dietary Guidelines to Physical Activity Security
Authors: Schwartz Juliano , Oh Paul , Perotto Maira B. , Rhodes Ryan E. , Firth Wanda , Bredin Shannon S. D. , Gaytán-González Alejandro , Warburton Darren E. R.
Published: September 2021

In light of new evidence on the prevention of chronic diseases and the elevated rates of overweight and obesity in Brazil and Canada, this critical review aims to interpret and synthesize current aspects regarding dietary and physical activity initiatives in both countries and make future recommendations. The pioneering work presented in the last Brazilian dietary guidelines has been called a model that can be applied globally, given its conceptualization of healthy eating that translates easily to practical guidance. The new Canadian Food Guide has incorporated similar aspects, also putting the country as a leader in dietary guidance. With these new recommendations, citizens in both Brazil and Canada have access to impactful evidence-informed nutritional guidelines. Both documents propose eating patterns that focus not only on health benefits, such as chronic disease prevention, but also incorporate well-being concerning cultural, economic, sociodemographic, biological, and ecological dimensions. A similar approach is required for physical activity to allow individuals to have attainable health and life goals and thereby fully enjoy their lives, regardless of geographical location, health status, and socioeconomic condition, a concept recently described as physical activity security. The wholistic dietary guidelines from both countries represent a change in paradigm in public health. Likewise, national evidence-based policies are warranted to reduce disparities in physical activity, allowing healthier and more active lifestyles for everyone.
What Can Thermal Imagery Tell Us About Glacier Melt Below Rock Debris?
Authors: Herreid Sam
Published: September 2021

Rock debris on the surface of a glacier can dramatically reduce the local melt rate, where the primary factor governing melt reduction is debris layer thickness. Relating surface temperature to debris thickness is a recurring approach in the literature, yet demonstrations of reproducibility have been limited. Here, I present the results of a field experiment conducted on the Canwell Glacier, Alaska, United States to constrain how thermal data can be used in glaciology. These datasets include, 1) a measured sub-daily “Østrem curve” time-series; 2) a time-series of high resolution thermal images capturing several segments of different debris thicknesses including the measurements from 1); 3) a thermal profile through a 38 cm debris cover; and 4) two Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) satellite thermal images acquired within 2 and 3 min of a field-based thermal camera image. I show that, while clear sky conditions are when space-borne thermal sensors can image a glacier, this is an unfavorable time, limiting the likelihood that different thicknesses of debris will have a unique thermal signature. I then propose an empirical approach to estimate debris thickness and compare it to two recently published methods. I demonstrate that instantaneous calibration is essential in the previously published methods, where model parameters calibrated only 1 h prior to a repeat thermal image return diminished debris thickness estimates, while the method proposed here remains robust through time and does not appear to require re-calibration. I then propose a method that uses a time-series of surface temperature at one location and debris thickness to estimate bare-ice and sub-debris melt. Results show comparable cumulative melt estimates to a recently published method that requires an explicit/external estimate of bare ice melt. Finally, I show that sub-pixel corrections to ASTER thermal imagery can enable a close resemblance to high resolution, field-based thermal imagery. These results offer a deeper insight into what thermal data can and cannot tell us about surface debris properties and glacier melt.
Place-Based Environmental Civic Science: Urban Students Using STEM for Public Good
Authors: Gallay Erin , Flanagan Constance , Parker Betsy
Published: September 2021

In the United States, Black and Latinx students are underrepresented in STEM courses and careers due to a dearth of culturally relevant opportunities, which in turn are connected to broader issues of social justice. Place-based environmental civic science offers potential for addressing these issues by enabling students to apply their STEM learning to mitigate local environmental problems. By civic science we refer to science in which all citizens, not just experts, engage for the public good. In this paper, we report on a study in which we followed middle-and high-school science and math classes in urban schools serving racial/ethnic minoritized students as they engaged in an innovative contextualized curriculum—a place-based civic science model in which students work with STEM community partners to address an environmental issue in their community. We draw from students’ open-ended reflections on what they learned from participating in place-based environmental civic science projects that could help their communities. Thematic analyses of reflections collected from 291 students point to beliefs in the usefulness of science to effect community change. Students articulated the science they learned or used in the project and how it could affect their community; they made references to real world applications of science in their project work and made links between STEM and civic contributions. In their own words, the majority of students noted ways that STEM was relevant to their communities now or in the future; in addition, a subset of students expressed changes in their thinking about how they personally could apply science to positively impact their communities and the ties between STEM and social justice. Analyses also point to a sense of confidence and purpose students gained from using STEM learning for their goals of community contribution. Results of this study suggest that focusing on local place as a foundation for students’ STEM learning and linking that learning to the civic contributions they can make, cultivates students’ perceptions of how they can use science to benefit their communities. Findings also suggest that engaging students in place-based civic science work provides effective foundations for nurturing STEM interest and addressing the underrepresentation of youth of color in STEM.
Extracellular Vesicles Inhibit Proliferation and Invasion of Ovarian Endometrial Stromal Cells and Their Expression of SF-1, ERβ, and Aromatase
Authors: Wang XiaoQing , Wu PeiLi , Li Xin , Zeng Cheng , Zhu JingWen , Zhou YingFang , Lu Ye , Xue Qing
Published: September 2021

Objective

Endometriosis is an estrogen-dependent chronic disease. The abnormal proliferation and invasion of ectopic stromal cells (ESCs) are important manifestations of endometriosis, and it is necessary to find safer and more effective treatments. Extracellular vesicles (EVs) derived from human umbilical cord mesenchymal stem cells (UC-MSCs) have been shown to be promising for the treatment of many diseases, except endometriosis. The main purpose of this study was to explore the effect of EVs derived from UC-MSCs on ESCs and evaluate the therapeutic value of EVs on endometriosis.

Study Design

Following the successful culture and identification of UC-MSCs, we collected the medium of UC-MSCs and extracted EVs by ultracentrifugation. Then, 120 μg/mL EVs were used to stimulate ESCs, which were collected to evaluate cell proliferation and invasion and expression of the estrogen-related proteins steroidogenic factor-1 (SF-1), estrogen receptors β (ERβ), and aromatase.

Results

Compared with the control group treated with isodose phosphate buffered saline (PBS), 120 μg/mL EVs exposure significantly decreased the expression of cyclin D1 (mRNA: n = 6, P = 0.02; protein: n = 6, P = 0.000) and matrix metalloproteinase (MMP) 9 (mRNA: n = 6, P = 0.04; protein: n = 6, P = 0.000) of ESCs, which were consistent with Cell Counting Kit-8(CCK-8) results (day 0: NC: 0.29 ± 0.04, 120 μg/mL EVs: 0.28 ± 0.04; day 1: NC: 0.42 ± 0.08, 120 μg/mL EVs: 0.32 ± 0.01; day 2: NC: 0.64 ± 0.07, 120 μg/mL EVs: 0.50 ± 0.05, P = 0.000; day 3: NC: 0.82 ± 0.09, 120 μg/mL EVs: 0.65 ± 0.07, P = 0.000; day 4: NC: 0.95 ± 0.11, 120 μg/mL EVs: 0.76 ± 0.07, P = 0.012; n = 6) and Transwell experiments (n = 6, P = 0.000). In addition, the expression of SF-1 (encoded by NR5A1; mRNA: n = 6, P = 0.000; protein: n = 6, P = 0.000), ERβ (encoded by ESR2; mRNA: n = 6, P = 0.000; protein: n = 6, P = 0.000), and aromatase (encoded by CYP19A1; mRNA: n = 6, P = 0.04; protein: n = 6, P = 0.000) in ESCs decreased significantly.

Conclusion

Taken together, the results show that 120 μg/mL EVs derived from UC-MSCs can effectively inhibit the proliferation and invasion of ESCs, as well as their expression of SF-1, ERβ and aromatase, and thus may lead to the alleviation of endometriosis.
Berberine Acts on C/EBPβ/lncRNA Gas5/miR-18a-5p Loop to Decrease the Mitochondrial ROS Generation in HK-2 Cells
Authors: Xu Jiang , Liu Linqing , Gan Lin , Hu Yuanyuan , Xiang Ping , Xing Yan , Zhu Jie , Ye Shandong
Published: September 2021

Background

Berberine (BBR) has therapeutic effect on diabetic nephropathy (DN), but its molecular mechanism is not completely clear.

Methods

The DN model was established to observe the therapeutic effect of BBR. The expression levels of lncRNA Gas5 were detected by PCR. The transcriptional regulation of CCAAT enhancer binding protein beta (C/EBPβ) on Gas5 was analyzed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) and luciferase reporter gene assay. The targeted regulation between Gas5 and miR-18a-5p and between miR-18a-5p and C/EBPβ 3′-untranslated region (3′-UTR) was also analyzed.

Results

In HG environment, BBR decreased the mitochondrial reactive oxygen species (ROS) generation and activated the C/EBPβ expression in HK-2 cells; C/EBPβ could combine with the reaction element on the promoter of Gas5 to promote its expression. Gas5 also inhibited the miR-18a-5p expression as competing endogenous RNA (ceRNA) and reduce the negative regulatory effect of miR-18a-5p on C/EBPβ. BBR could activate C/EBPβ/peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) signal pathway, regulate mitochondrial energy metabolism, and inhibit ROS production and apoptosis by activating C/EBPβ/Gas5/miR-18a-5p positive feedback loop in HG environment. It also showed that BBR alleviated streptozotocin (STZ) induced renal injury in DN rats in vivo.

Conclusions

This study suggested that BBR could regulate the mitochondrial ROS generation by activating the positive feedback loop of C/EBPβ/Gas5/miR-18a-5p.
Nucleotide Metabolism Behind Epigenetics
Authors: Suganuma Tamaki , Workman Jerry L.
Published: September 2021

The mechanisms of epigenetic gene regulation—histone modifications, chromatin remodeling, DNA methylation, and noncoding RNA—use metabolites as enzymatic cofactors and substrates in reactions that allow chromatin formation, nucleotide biogenesis, transcription, RNA processing, and translation. Gene expression responds to demands from cellular processes that use specific metabolites and alters or maintains cellular metabolic status. However, the roles of metabolites—particularly nucleotides—as regulatory molecules in epigenetic regulation and biological processes remain largely unknown. Here we review the crosstalk between gene expression, nucleotide metabolism, and cellular processes, and explore the role of metabolism in epigenetics as a critical regulator of biological events.
Endoplasmic Reticulum Stress Induced Proliferation Remains Intact in Aging Mouse β-Cells
Authors: Snyder Jarin T. , Darko Christine , Sharma Rohit B. , Alonso Laura C.
Published: September 2021

Aging is associated with loss of proliferation of the insulin-secreting β-cell, a possible contributing factor to the increased prevalence of type 2 diabetes in the elderly. Our group previously discovered that moderate endoplasmic reticulum (ER) stress occurring during glucose exposure increases the adaptive β-cell proliferation response. Specifically, the ATF6α arm of the tripartite Unfolded Protein Response (UPR) promotes β-cell replication in glucose excess conditions. We hypothesized that β-cells from older mice have reduced proliferation due to aberrant UPR signaling or an impaired proliferative response to ER stress or ATF6α activation. To investigate, young and old mouse islet cells were exposed to high glucose with low-dose thapsigargin or activation of overexpressed ATF6α, and β-cell proliferation was quantified by BrdU incorporation. UPR pathway activation was compared by qPCR of target genes and semi-quantitative Xbp1 splicing assay. Intriguingly, although old β-cells had reduced proliferation in high glucose compared to young β-cells, UPR activation and induction of proliferation in response to low-dose thapsigargin or ATF6α activation in high glucose were largely similar between young and old. These results suggest that loss of UPR-led adaptive proliferation does not explain the reduced cell cycle entry in old β-cells, and raise the exciting possibility that future therapies that engage adaptive UPR could increase β-cell number through proliferation even in older individuals.
An Experimental Study on the Formation of Natural Gas Hydrate With Thermodynamic Inhibitors
Authors: Wei Na , Xie Cuiying , Sun Wantong , Li Haitao , Jiang Lin , Zhang Shenghui
Published: September 2021

Gas hydrates formed in the conditions of high pressure and low temperature in deep sea and in the process of oil and gas transportation, natural gas hydrate (NGH), will seriously affect the safety of drilling and completion operations and marine equipment and threaten the safety of drilling platform. How to prevent the hydrate formation in the process of oil and gas production and transportation has become an urgent problem for the oil and gas industry. For this reason, in view of the formation of NGH in the process of drilling and producing marine NGH, the phase equilibrium calculation research of NGH formation was carried out, the mathematical model of gas hydrate formation phase equilibrium condition was established, and the experimental research on NGH formation was carried out through adding different thermodynamic inhibitors. The experimental phenomena show that, first, the stirring speed has little effect on the inhibition of hydrate formation. Second, when the pressure is 10 MPa and the volume concentration of inhibitor is 1, 3, 5, and 7%, the supercooling degree of hydrate formation is 1.81, 8.89, 11.09, and 9.39°C, respectively. Third, when the volume concentration of inhibitor is 1, 3, 5, and 7%, the induction time of hydrate formation is 10328, 14231, 19576, and 24900 s, respectively. As the polymer molecules in the inhibitor reduce the activity of water in the system and fill the cavity structure of the hydrate, they reduce the generation conditions of NGH and break the original phase equilibrium conditions when NGH is generated, thus forming NGH at a lower temperature or higher pressure.
Review of Large-Scale Biochar Field-Trials for Soil Amendment and the Observed Influences on Crop Yield Variations
Authors: Vijay Vandit , Shreedhar Sowmya , Adlak Komalkant , Payyanad Sachin , Sreedharan Vandana , Gopi Girigan , Sophia van der Voort Tessa , Malarvizhi P , Yi Susan , Gebert Julia , Aravind PV
Published: September 2021

Increasing pressure on farming systems due to rapid urbanization and population growth has severely affected soil health and fertility. The need to meet the growing food demands has also led to unsustainable farming practices with the intensive application of chemical fertilizers and pesticides, resulting in significant greenhouse gas emissions. Biochar, a multifunctional carbon material, is being actively explored globally for simultaneously addressing the concerns related to improving soil fertility and mitigating climate change. Reviews on biochar, however, mainly confined to lab-scale studies analyze biochar production and its characteristics, its effects on soil fertility, and carbon sequestration. The present review addresses this gap by focusing on biochar field trials to enhance the current understanding of its actual impact on the field, w.r.t. agriculture and climate change. The review presents an overview of the effects of biochar application as observed in field studies on soil health (soil’s physical, chemical, and biological properties), crop productivity, and its potential role in carbon sequestration. General trends from this review indicate that biochar application provides higher benefits in soil properties and crop yield in degraded tropical soils vis-a-vis the temperate regions. The results also reveal diverse observations in soil health properties and crop yields with biochar amendment as different studies consider different crops, biochar feedstocks, and local climatic and soil conditions. Furthermore, it has been observed that the effects of biochar application in lab-scale studies with controlled environments are not always distinctly witnessed in corresponding field-based studies and the effects are not always synchronous across different regions. Hence, there is a need for more data, especially from well-designed long-term field trials, to converge and validate the results on the effectiveness of biochar on diverse soil types and agro-climatic zones to improve crop productivity and mitigate climate change.
Experimental and Numerical Investigation of Composite Phase Change Materials for Building Energy Saving
Authors: Zhang Guanhua , Guo Yuqian , Liu Zhiqiang , Lu Wei , Yan Xiaoyu , Du Yanping
Published: September 2021

Composites composed of paraffin wax and copper foam with porosity of 95 % and pore density of 15 ppi, 30 ppi, and 50 ppi were prepared by the melt impregnation method. Two building models of the same size were made using gypsum board. The roof of the experimental model was covered with 50ppi copper foam /paraffin composite phase change board, and the roof of the reference model was covered with foam insulation board. The heat transfer experiment was conducted with a constant heat flux. EnergyPlus software was also employed to simulate the energy saving effects of the building models. The size and material of the model established during the simulation are consistent with the experimental model. Experimental results showed that the phase change composite board delayed the highest indoor temperature for 1.5 h, and it also reduced the indoor maximum temperature by 1.2° and the indoor energy consumption by 21 %. The simulation results showed that the phase change composites board delayed the appearance of the highest indoor temperature by 1.3 h and reduced the highest indoor temperature by 1.1°C, and the maximum indoor energy consumption decreased by 19 %. The copper foam/paraffin composite material can effectively improve the thermal comfort and reduce the energy consumption of the building model.
A Hierarchical Game Theory Based Demand Optimization Method for Grid-Interaction of Energy Flexible Buildings
Authors: Xu Jinyi , Yan Chengchu , Xu Yizhe , Shi Jingfeng , Sheng Kai , Xu Xiaoping
Published: September 2021

Building demand-side management is an effective solution for relieving the peak and imbalance problems of electrical grids. How to explore the energy flexibility of buildings and to coordinate a variety of buildings with different energy flexibilities for effective interactions with smart grids are a great challenge. This paper proposes a game theory–based hierarchical demand optimization method for energy flexible buildings for achieving better grid interactions. This method consists of two optimization strategies at the grid and building levels. At the grid level, a demand-price interaction model for buildings and the grid is established to identify the Nash equilibrium solutions based on game theory; these solutions are used to determine the optimized energy demand of buildings and the associated electricity prices by accommodating the interests of all participants involved. At the building level, three types of buildings with different energy flexibilities are investigated to analyze the influence of building management strategies on grid interactions. The effectiveness of the proposed method is verified in a simulated case study. The results show that the optimization method can reduce building operational cost by 3–18%, reduce the fluctuation of the power grid by 30–50%, and ensure that the power grid increases income by 8–20%.
The STEM Enhancement in Earth Science “Mosquito Mappers” Virtual Internship: Outcomes of Place-Based Engagement with Citizen Science
Authors: Cho Holly , Low Russanne D. , Fischer Heather A. , Storksdieck Martin
Published: September 2021

In this paper, we describe a virtual high school earth science enhancement program that embeds a citizen science experience within a scientist-mentored research internship. We demonstrate the success of the program as measured by knowledge of pertinent science concepts and processes, and changes in future career orientation, and explore the role of place-based citizen science in interns’ experience of their research projects. The STEM Enhancement in Earth Science (SEES) Mosquito Mappers Virtual Internship connected high school interns with research opportunities applying NASA Earth Observations to the global health threat of mosquito-vector borne diseases. The interns engaged in 120–150 h of research working closely with NASA subject matter expert mentors, and making use of the GLOBE Observer citizen science infrastructure to collect and analyse data. In the virtual format, interns were able to participate in activities synchronously or asynchronously through an online learning platform, web conferencing software and social media. Students developed and completed either an independent or small team research project leveraging data collected using the place-based citizen science mobile application, GLOBE Observer. We found that participants were motivated by conducting fieldwork in and around their communities using the GLOBE Observer app. Local fieldwork enabled the student researchers to apply their prior knowledge of their natural and built landscapes, while also contributing to the reduction of mosquito-borne disease risk in their communities. This finding highlights the benefit of coupling citizen science to a virtual internship. Interns shared that their interest in research, and research in the earth sciences specifically, increased as a result of participating in the internship program. By leveraging citizen science data collection and reporting tools, existing data, and analysis tools, the program represented a robust, cost effective research experience conducted in a virtual environment, lowering barriers to participation and broadening access to STEM enhancement opportunities for all.
Rewetting and Drainage of Nutrient-Poor Peatlands Indicated by Specific Bacterial Membrane Fatty Acids and a Repeated Sampling of Stable Isotopes (δ15N, δ13C)
Authors: Groß-Schmölders Miriam , Klein Kristy , Birkholz Axel , Leifeld Jens , Alewell Christine
Published: September 2021

Peatland degradation impairs soil functions such as carbon storage and the existence of biodiversity hotspots. Therefore, and in view of the ongoing climate change, an efficient method of evaluating peatland hydrology and the success of restoration efforts is needed. To understand the role of microbial groups in biogeochemical cycling, gaseous loss and isotopic fractionation that lead to specific isotopic depth patterns (δ13C, δ15N), we integrated previously published stable isotope data with a membrane fatty acid (mFA) analysis related to various microbial groups that are known to be common in peatlands. We performed two sampling campaigns to verify the observed stable isotope depth trends in nutrient-poor peatlands in Northern Europe. Cores were taken from adjacent drained (or rewetted) and undrained sites. Fungal-derived mFA abundance was highest in the uppermost part of the drained layer. We found increasing bacterial-derived mFA concentrations with depth peaking in the middle of the drained layers, which correlates with a δ15N peak of bulk material. The results support our hypothesis that changing peatland hydrology induce a shift in microbial community and metabolism processes and is therefore also imprinted in stable isotope values. Under waterlogged conditions overall levels of microbial-derived mFAs were generally low. Drained layers showed simultaneous changes in microbial abundance and composition and depth trends in stable isotope bulk values. Bacteria, particularly acidobacteria, can be expected to dominate increased denitrification with low oxygen saturation accompanied by increased δ15N bulk values in the remaining substrate. Interestingly, cores from recent rewetted peatlands show no depth trend of δ15N in the layers grown under rewetting conditions; this is congruent with relatively low concentrations of microbial-derived mFAs. Hence, we conclude that stable isotopes, especially δ15N values, reflect changing microbial metabolic processes, which differ between drained and undrained - and especially also for recent rewetted–peatlands. As today stable isotope measurements are routine measurements, these findings enable us to get cost- and time efficient reliable information of drainage and restoration success.