bluuebunny/crossref_metadata_2025 huggingface dataset:
Sepse relacionada a injúria renal aguda: Uma revisão de literatura
Authors: Mendonça Willian Júnio Rodrigues , Silva Talita Maques da , Ledo Ana Cláudia de Brito , Silva Victor Augusto Vaz
Published: December 2024

Introdução: Sepse é uma disfunção orgânica causada a partir de uma de uma resposta a uma infecção, um dos órgãos mais afetados é o rim, causando injúria renal aguda (IRA). O objetivo deste estudo é relacionar as evidências mais recentes de compreensão da fisiopatologia e do uso de biomarcadores de sepse relacionada a injúria renal aguda. Metodologia: Essa pesquisa é uma revisão narrativa de literatura, que buscou abordar a injúria renal aguda na sepse. A pesquisa usou as bases de dados Scielo, PubMed MEDLINE e LILACS, com as palavras chaves “sepse” e “injúria renal aguda”. Resultado e Discussão: Destaca-se uma discussão indicando que a IRA induzida pela sepse leva em consideração critérios como de sepse da Surviving Sepsis Campaign e os de IRA da KDIGO. Biomarcadores são importantes quando há IRA relacionada à sepse. O seu diagnóstico é feito por meio de ferramentas como o SOFA, SIRS e NEWS, porém o qSOFA não é mais utilizado nessa identificação. O tratamento é feito com antibióticos de amplo espectro, com cautela no uso de agentes nefrotóxicos, e norepinefrina como vasopressor de primeira linha. Conclusão: Entende-se, portanto, que por meio desse estudo foi possível compreender mais sobre a fisiopatologia da sepse relacionada a injúria renal aguda, sendo prevalente a inflamação como fator essencial para a IRA. Ademais, ainda há uma prevalência de pessoas que necessitam de hemodiálise ou substituição renal após sepse com injúria renal aguda, tornando evidente a necessidade de estudo e capacitação dos profissionais da área.
Conhecimento dos estudantes de Radiologia sobre o descarte inadequado dos filmes radiográficos
Authors: Laurentino Marylia Kelly da Silva , Gondim Josué Brito , Pereira Mayra Gabrielly Costa , Nunes Tatiana Lima , Nóbrega Lidia Pinheiro da , Bezerra Anne Milane Formiga , Abrantes Marcela Meira Ramos
Published: December 2024

O trabalho conta brevemente, acerca da utilização de películas radiográficas em impressões de exames no diagnóstico por imagem, acarretando em futuros malefícios relacionados ao uso de compostos pesados, utilizados na fabricação da película radiográfica que é usada para a impressão de radiografias e seus futuros malefícios relacionados aos compostos do filme utilizado no processamento de impressão. O objetivo do presente estudo é demonstrar o tamanho da proporcionalidade que um filme radiográfico pode causar ao meio ambiente quando não realizado um descarte adequado, pois durante o processo de fabricação das películas são aplicadas substâncias que contêm metais pesados que prejudicam a saúde humana. O resultado do estudo contou com diferentes respostas dos participantes, o que levou a grande relevância sobre a falta de conhecimento tanto sobre o tipo de lixo como também a sua forma de descartar, por parte de muitos. Para alcançar esse objetivo, foi realizado pesquisa de campo utilizando de início como base de dados o questionário semiestruturado para nível de conhecimento de alunos da área que tem contato direto com acessórios e materiais da radiologia como também ações em estabelecimentos que fazem uso de resíduos radioativos tendo assim como proposta pontos de coletas, onde a sociedade pudesse descartá los em locais específicos para seguinte utilização desse material, tornando o reciclável.
Uso de Fitoterápicos como estratégia de controle de doenças não transmissíveis
Authors: Paula Kallyza De , Santos Karine Almeida , Santos Jânio Sousa
Published: December 2024

A abordagem integrativa na saúde pública tem se destacado como uma estratégia eficaz no tratamento de doenças crônicas não transmissíveis (DCNT), oferecendo alternativas complementares aos métodos tradicionais e promovendo a melhoria da qualidade de vida. As DCNT, como doenças cardiovasculares e câncer, representam um desafio global para os sistemas de saúde. A integração da fitoterapia no tratamento das DCNT leva em consideração os aspectos físicos, emocionais, sociais e ambientais das pessoas, promovendo uma abordagem mais integral e centrada no paciente. O objetivo do presente estudo é apresentar formas de controle de doenças não transmissíveis por meio do uso de medicamentos fitoterápicos com sua própria saúde. Realizou-se uma pesquisa de revisão bibliográfica e, para tal foram consultadas bases de dados científicas como: Scielo, Scopus, Web of Science, LILACS e PubMed. Os descritores em saúde utilizados foram: "fitoterapia", "doenças não transmissíveis". Os resultados apontam que o uso de fitoterápicos pode ser benéfico no controle de doenças como diabetes, hipertensão, doenças cardiovasculares e obesidade. As propriedades farmacológicas das plantas medicinais, como as atividades anti-hiperglicemiante, antioxidante e anti-inflamatória, contribuem para a prevenção e o tratamento eficaz dessas condições. Além disso, a fitoterapia oferece uma alternativa mais acessível e menos invasiva em comparação aos medicamentos sintéticos, o que é particularmente relevante para populações de baixa renda.
A informatização do processo de credenciamento para docentes na Polícia Militar de Alagoas (PMAL)
Authors: Viégas Raquel Morais da Silva , Silva Júnior Edival Lima da
Published: December 2024

A Matriz Curricular Nacional é um referencial teórico-metodológico que orienta as ações formativas dos profissionais da área de segurança pública. No âmbito educacional, o processo de credenciamento de instrutores na PMAL é regulamentado através do edital nº 027/2015 publicado no BGO nº 230, de 16 de dezembro de 2015, baseado em critérios burocráticos, como tempo de experiência e quantidade de títulos acadêmicos. Com os avanços tecnológicos, as inscrições, anteriormente realizadas apenas presencialmente, são feitas exclusivamente através do Sistema Eletrônico de Informações. À vista disso, o Comandante Geral da PMAL, com o advento à Nova Lei de Licitações e Contratos Administrativos, instituiu uma comissão técnica para idealizar propostas ao edital de credenciamento para instrutores e monitores, estabelecendo critérios e procedimentos para a seleção, qualificação e acompanhamento desses profissionais. Neste contexto, o objetivo central deste estudo foi integrar um software à Central de Acesso da PMAL, com ênfase no processo de credenciamento mencionado. Para tanto, empregou-se a pesquisa exploratória e descritiva, por proporcionar a disseminação de informações acerca de uma temática, delineando-a e aprofundando o conhecimento, ao mesmo tempo em que observa e analisa a ocorrência de dados. Como efeito, evidenciou-se uma unanimidade acerca do reconhecimento dos benefícios e melhorias provenientes da inserção do sistema informatizado nas Escolas da PMAL. Também, constatou-se um alto potencial para otimizar os critérios burocráticos, bem como reduzir o tempo de espera dos candidatos a docentes, melhorando assim a eficácia e a eficiência neste processo.
Estratégias nutricionais na síndrome do ovário policístico
Authors: Silva Tatiane Bento da , Bello Sergio Ricardo de Brito , Pazello Camila Tedeschi , Zarpellon Raquel Simone Maccarini , Venturelli Amanda Caroline , Souza Rosana Menezes de , Silva Roxane Wirschum
Published: December 2024

A síndrome do ovário policístico (SOP) é uma doença endócrina crônica caracterizada pela presença de pequenos cistos nos ovários. Hirsutismo, resistência insulínica, queda de cabelo, distúrbios menstruais, infertilidade e acne são manifestações frequentemente associadas a essa doença. Diante da complexidade do quadro, o objetivo da presente revisão integrativa foi levantar achados científicos que pudessem dar sustentação à nutrição como uma estratégia coadjuvante no tratamento da SOP. Para tanto, foram levantados 34 artigos científicos publicados em língua portuguesa e inglesa nas bases de dados National Library of Medicine (PubMed) e Google Acadêmico (Scholar). Mudanças no estilo de vida associadas a dietas que priorizam uma alimentação com baixo índice glicêmico, adequada do ponto de vista qualitativo e associada a prática da suplementação direcionada para a SOP, reduzem o quadro sintomatológico do paciente, impactando na redução das comorbidades e melhorando o estado geral do paciente.
A influência do Mestrado Profissional em Ensino de Biologia - PROFBIO na vivência do Ensino Investigativo na formação de licenciandos em Ciências Biológicas
Authors: Cardoso Thâmara Chaves , Machado Vera Mattos , Lima Michelle Mara de Oliveira
Published: December 2024

Esse artigo é parte de uma pesquisa de Tese de Doutorado em Ensino de Ciências da Universidade Federal do Mato Grosso do Sul – UFMS. O objetivo do presente artigo é apresentar parte da pesquisa de doutorado voltada para compreender a vivência das práticas investigativas na Formação de Professores em uma Instituição de Ensino Superior pública do Estado do Piauí. Ele é resultado de um estudo qualitativo que realizou uma Análise Documental no Projeto Pedagógico de Curso, e aplicação de questionários com coordenador de curso, docente formador em Estágio Supervisionado e quatro licenciandos, sobre a temática Ensino Investigativo na formação docente. Os dados foram analisados mediante a Análise do Discurso de Eni Orlandi. A vivência do EI na instituição está ocorrendo de forma interessante, porém encontra-se limitada à ação intencional de alguns formadores. O EI não é contemplado no currículo do curso. Essa ação docente é produto da influência da Formação Continuada de alguns formadores da instituição no Mestrado Profissional em Ensino de Biologia - PROFBIO. Consideramos a influência do PROFBIO na instituição bastante positiva na vivência do EI durante a formação dos licenciandos.
O filme “Elementos” como ferramenta terapêutica Junguiana: Um relato de caso
Authors: Souza Handria Inandy da Silva , Santos Gabriela Stefani Moraes Lopes
Published: December 2024

A presente pesquisa versa sobre o uso da Arteterapia Junguiana no atendimento de psicoterapia a uma criança com demanda de agressividade em relação à mãe, após separação dos pais em cumprimento de Medida Protetiva Judicial por motivo de violência doméstica tipo psicológica. O acompanhamento da criança foi realizado na Clínica Escola de Psicologia da Faculdade Mauricio de Nassau, Vitoria da Conquista, Bahia, Brasil, no primeiro semestre do ano de 2023. Trata-se de um caso clínico cuja intervenção foi realizada através da arteterapia à luz da Psicologia Analítica Junguiana. O objetivo deste estudo foi analisar a eficácia da arteterapia como ferramenta terapêutica infantil em casos de crianças que se sentem inseguras em falar sobre suas emoções impedindo a associação livre, a transferência e o acesso do subconsciente ao consciente. Foram realizadas sessões com a criança utilizando a arte como linguagem simbólica, não verbal e metafórica através do Filme Elementos e os Arquétipos identificados pela criança, além de outros materiais diversos para produção artísticas, possibilitando sua percepção comportamental, suas emoções e sentimentos em relação aos seus familiares e ao divórcio. Os resultados apontaram que a criança se expressava através da agressividade por sentir-se culpada, punida e sofrer alienação parental por parte do pai em relação a sua mãe.
Evaluation of the Relationship Between Rainwater Harvesting and Landscape Plant Water Consumption
Authors: Mısırlı Nilay , Şişman Elif Ebru
Published: December 2024

If rainwater is not used, it is considered waste and ends up as surface water in underground resources or flows into oceans. In view of dwindling water resources, rainwater should not only flow as surface water but should be reused to conserve groundwater and mains water. To achieve this, rainwater must be collected and solutions produced on site. When we look at water consumption rates, we realize that a large amount of water is significantly used for the irrigation of landscaped areas. In addition, the water requirements of plants are often not known and water is wasted through unconscious irrigation. This study aims to provide the right amount of irrigation by showing the water requirements of plants according to their species. At the same time, it aims to provide the right amount of irrigation by showing the water requirements of plants according to their species. These two main objectives are aimed at learn the needs of plants and at the same time ensuring efficient water use. In this study, it is aimed to contribute to the water cycle by reusing rainwater. Various roofing and paving materials were identified in the study area. The amount of rainwater to be collected from the different materials within the study area was calculated using the Rational Method and the water consumption of each plant was calculated using the CropWat 8.0 program. In conclusion, the amount of rainwater collected on the entire campus was calculated as 494,000 m3per year and the amount of irrigation water required by the plants was 54,530 m3per year. This data shows that the amount of rainwater collected corresponds to the water consumption of the landscape plants. The rainwater harvested on campus is fed into tanks. The rainwater collected on the campus is channelled into tanks. The volume of the tanks was calculated. In addition to the stored rainwater, solutions were developed on-site using sustainable methods for the remaining rainwater. Plants with low or medium water requirements are recommended for use in new landscape areas.
Analisis Formalistik Lukisan Djoko Pekik Berjudul "Pawang Pun Kesurupan"
Authors: Syai'in Ahmad , Prasetyo Abdul Rahman
Published: December 2024

Djoko Pekik memiliki gaya lukisannya yang beraliran realis ekspresif. Kerap membumbui nilai-nilai kerakyatan di hampir setiap lukisannya. Karyanya banyak memuat kritik sosial hingga tragedi politik. Oleh karena itu, lukisannya begitu berpengaruh. Memulai karier lukisnya sejak 1958 dengan masuk ke ASRI Yogyakarta, Djoko Pekik terus merevolusi diri hingga kini namanya begitu harum di dunia seni. Dia bahkan sempat mendirikan Sanggar Bumi Tarung pada 1961 bersama sejumlah kawannya. Ide kolektif kesenian itu kemudian diketahui berafilisasi dengan Lembaga Kebudayaan Rakyat (Lekra). Tujuan kritik formalistik ini untuk menjabarkan konfigurasi aspek-aspek formal dan nilai-nilai luhur yang berkaitan dengan unsur pembentukan karya lukis. Sasaran kritik seni akan lebih cenderung pada unsur-unsur visual terdiri warna, garis, dan tekstur. Metode kritik seni yang digunakan berupa kritik formalistik dengan tahapan deskripsi, tahapan analisis formal, tahapan interpretasi, dan tahapan evaluasi. Hasil dari kritik formalistik ini berupa data analisis visual karya lukis dan interpretasi nilai-nilai luhur yang berkaitan dengan unsur pembentukan karya lukis.
Perancangan Media Sosial Instagram Sebagai Media Promosi Wisata Kuliner Karesidenan Bojonegoro
Authors: Ikhsan Tsari Rifdah Tsani , Sarjono Sarjono
Published: December 2024

Karesidenan Bojonegoro daerah yang sering dilewati pendatang. Namun terdapat kendala yaitu pendatang tidak tahu harus singgah dan memilih makan sesuai selera mereka. Kabupaten karesidenan Bojonegoro memiliki berbagai macam kuliner dan oleh-oleh yang enak dan belum tentu dapat dijumpai di kota lain. Oleh sebab itu dilakukan perancang media sosial Instagram sebagai media promosi wisata kuliner karesidenan Bojonegoro yang memuat ilustrasi makanan, deksripsi, informasi tambahan setiap destinasi wisata kuliner. Metode perancangan menggunakan model perancangan Sanyoto (2006), dengan tahapan latar belakang, identifikasi, data perusahaan dan produk, Data permasaran, analisis, sintesis, konsep perancangan, konsep tata desain, dan desain final. Proses produksi dilakukan melalui pembuatan layout gagasan dan layout kasar secara manual, layout lengkap secara digital dan finishing. Dari penelitian ini dihasilkan media utama berupa feed Instagram dan media pendukung pameran meliputi, banner, poster, infografis, pamphlet, brosur leflet, katalog digital, website, display iklan sosial media, dan merchandise totebag.
Perancangan Tempat Penyimpanan Makanan dan Kebutuhan Kucing untuk Generasi Z di Lingkungan Urban
Authors: Candra Hardian Purnama , Fajrin Muhammad Alief Athallah , Yulardi Audit , Maharany Gunanda Tiara
Published: December 2024

Penelitian ini mengusung sebuah produk berupa tempat penyimpanan makanan dan kebutuhan kucing yang ditujukan khusus untuk generasi Z yang memiliki kucing di apartemen atau rumah sewa. Dimulai dari kurangnya opsi penyimpanan yang tersedia untuk keperluan kucing, seringkali menyebabkan kekacauan dalam penyimpanan bagi pemilik kucing, mengganggu tampilan ruangan, dan menghambat pengaturan ruang yang efisien. Tujuan produk ini adalah memberikan kemudahan dalam mengorganisir keperluan dan makanan kucing, serta menyediakan tempat yang terstruktur untuk memberi makan serta menciptakan lingkungan yang tertata dan nyaman. Proses desain melibatkan pengumpulan data melalui kuesioner dari pemilik kucing dan membuat persona pengguna untuk memahami kebutuhan dan preferensi pengguna. Hasilnya adalah wadah penyimpanan minimalis bergaya Jepang yang efisien, estetis, dan sesuai dengan gaya hidup urban. Dilengkapi dengan kompartemen untuk makanan dan kebutuhan kucing, serta area pemberian makan. Produk ini dirancang untuk mengoptimalkan fungsi dan kenyamanan bagi pengguna, memberikan kemudahan dalam mengatur perlengkapan dan makanan kucing, serta meningkatkan estetika ruangan.
Migrasome regulator TSPAN4 shapes the suppressive tumor immune microenvironment in pan-cancer
Authors: Wang Lin-jian , Xu Ruiyan , Wu Yangyang
Published: December 2024

Background

Migrasomes are newly identified organelles on the retracting fibers of migrating cells, involved in releasing signaling molecules, expelling damaged mitochondria, and facilitating intercellular communication through phagocytosis. TSPAN4, a key regulator of migrasome formation, is a valuable marker for visualizing these organelles. However, its role in cancer remains unclear.

Methods

We analyzed TSPAN4 expression and its prognostic significance across multiple cancers using TCGA Pan-Cancer (PANCAN), and TCGA TARGET GTEx datasets. The relationship between TSPAN4 and tumor heterogeneity, stemness, and the immunosuppressive tumor microenvironment was explored through RNA-seq and scRNA-seq data. In addition, we examined TSPAN4's role in glioma, focusing on migrasome formation, cell proliferation, and macrophage polarization.

Results

Our analysis reveals that TSPAN4 is aberrantly expressed in various tumors, likely linked to its methylation status. It correlates with tumor heterogeneity, stemness, and a suppressive immune microenvironment. In glioma, TSPAN4 enhances cell proliferation and promotes macrophage polarization toward the immunosuppressive M2 phenotype.

Conclusions

TSPAN4, as a migrasome regulator, plays a crucial role in shaping the immunosuppressive tumor microenvironment in pan-cancer.
A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
Authors: Shi Lihua , Yu Mingcan , Jin Ying , Chen Peng , Mu Guangmao , Tam Susan H. , Cho Minseon , Tornetta Mark , Han Chao , Fung Man-Cheong , Chiu Mark L. , Zhang Di
Published: December 2024

Introduction

Thymic stromal lymphopoietin (TSLP) is a master regulator of allergic inflammation against pathogens at barrier surfaces of the lung, skin, and gut. However, aberrant TSLP activity is implicated in various allergic, chronic inflammation and autoimmune diseases and cancers. Biologics drugs neutralizing excess TSLP activity represented by tezepelumab have been approved for severe asthma and are being evaluated for the treatments of other TSLP-mediated diseases.

Methods and results

In this study, we discovered and characterized a novel humanized anti-TSLP antibody TAVO101 with high binding affinity to human TSLP, which blocks TSLP binding to its receptor complexes on cell surface. TAVO101 showed potent neutralization of TSLP activities in the TSLP-driven STAT5 reporter assay and cell proliferation assay. Results from ex vivo studies showed that TAVO101 neutralized TSLP-mediated CCL17 release from primary human CD1c+ dendritic cells and proliferation of activated CD4+ T cells. In addition, TAVO101 showed strong efficacy in both TSLP/OVA-induced asthma and imiquimod induced psoriasis models in hTSLP/hTSLPR double knock-in mice. We further conducted Fc engineering to optimize TAVO101 antibody with reduced affinity to Fcγ receptors and C1q protein but with increased affinity to FcRn receptor for half-life extension.

Discussion

By recognizing a different epitope, similarly potent neutralization of TSLP activities, and longer circulating half-life than tezepelumab, novel anti-TSLP antibody TAVO101 offers a potential best-in class therapeutics for various TSLP-mediated diseases.
SOX13 as a potential prognostic biomarker linked to immune infiltration and ferroptosis inhibits the proliferation, migration, and metastasis of thyroid cancer cells
Authors: Ma Yan-yan , Zhou Wei-ye , Qian Yue , Mu Ying-ying , Zhang Wei
Published: December 2024

Background

SOX13 is a transcription factor belonging to the SOX family. SOX proteins are critical regulators of multiple cancer progression, and some are known to control carcinogenesis. Nevertheless, the functional and clinical significance of SOX13 in human thyroid cancer (THCA) remain largely unelucidated.

Methods

Data on SOX13 expression were obtained through The Cancer Genome Atlas together with Gene Expression Omnibus. Co-expression, differential expression, and functional analyses of genes were investigated by databases. Associations between SOX13 levels, immune infiltration, ferroptosis, and immune checkpoint gene levels were analyzed. Genetic changes in SOX13 were investigated using CBioPortal. Associations between SOX13 levels and THCA clinicopathological features were analyzed and nomogram modeling for diagnostic and prognostic prediction. The influence of SOX13 on proliferation, migration, and metastasis was determined in KTC-1 and TPC-1 cell lines.

Results

SOX13 was significantly lower in THCA tumors compared to controls. In addition, upregulated SOX13 gene mutation were evident in thyroid cancer. SOX13-associated genes exhibited differential expression in pathways associated with thyroid cancer development. Significant associations were found between SOX13 levels, immune infiltration, ferroptosis, and immune checkpoint genes in THCA tissue. SOX13 levels correlated with THCA stage, histologic grade, and primary neoplasm focus types, and independently predicted overall and progression-free intervals. SOX13 expression effectively distinguished between tumor and normal thyroid tissue. Spearman correlations highlighted a significant relationship between SOX13 and ferroptosis-associated genes. Overexpression of SOX13 enhances the inhibition of RSL3 (iron death activator) on the cell viability of TPC-1. Higher SOX13 levels in Thyroid cancer cells may lead to reduced proliferation, migration, and metastasis by regulating ferroptosis.

Conclusion

Reduced SOX13 expression inversely impacts patient prognosis. In addition, SOX13 strongly regulates cancer immunity and Ferroptosis. Hence, SOX13 has great promise as a bioindicator for both thyroid cancer prognosis and immune cell invasion.
Has green innovation in marine-related enterprises reduced environmental pollution? –a perspective based on executives’ environmental awareness
Authors: Wang Changrong , Dong Xiaopeng
Published: December 2024

This study investigates the direct impact of green innovation in marine-related enterprises on pollution emissions, addressing a significant gap in existing research regarding the role of executives’ environmental awareness factor. Using a sample of 2,580 marine-related firm-year observations from the 2008-2022 period, we find that green innovation and improved environmental awareness among management can significantly reduce water pollution and air pollutants in enterprises and reduce the environmental problems caused by marine-related businesses. Moreover, enhancing environmental awareness within management can effectively substitute for the inhibitory effects of green innovation on pollution emissions. We conduct a heterogeneity test, and it proves that the inhibitory effects of green innovation and executives’ environmental awareness vary across industries and ownership types. These findings contribute to the literature on the sustainable development of marine enterprises.
Fe-bearing magnesium silicate glasses for potential supplementary cementitious applications
Authors: Jiang Chuqing , Santos Hellen Silva , Yliniemi Juho , Lindén Johan , Ramteke D. D. , Illikainen Mirja , Cheeseman Christopher , Kinnunen Paivo
Published: December 2024

Supplementary cementitious materials (SCMs) are used to minimize CO2 emissions associated with cement production. However, their global supply is insufficient to meet the growing market demand for cement and concrete, being essential to develop alternative SCMs based on abundant waste streams and low-cost resources. Fe-bearing Mg-based glasses are promising candidates with the potential to utilize high-volume feedstocks rich in Fe and Mg, but their effectiveness relies on deep understanding of the relationship between glass composition, reactivity, and pozzolanic properties. In this study, Fe-Mg silicate glasses with varying Fe concentrations were precisely engineered through a sol-gel route to better understand the impact of Fe on the glass structure and reactivity. While Fe3+ typically acts as a glass network former, it was observed to also function as an intermediate cation, behaving either as a network former or modifier. Glass reactivity was assessed through aqueous dissolution tests, revealing that the composition and chemical environment of Fe3+ within the glass network significantly influence the dissolution behavior. The introduction of Fe into Mg-Si glasses increased overall reactivity, potentially due to Fe-induced phase separation and the increasing of [FeO6] octahedra sites at higher Fe concentrations, which was also associated to network depolymerization. These findings deepen the understanding of the role of Fe3+ in magnesium silicate glasses, provide key insights into optimizing glass reactivity by fine-tuning the composition, and indicate the potential of these glasses as promising SCMs.
Identification of key genes related to growth of largemouth bass (Micropterus salmoides) based on comprehensive transcriptome analysis
Authors: Hu Dayan , Jian Jieliang , Zhang Jinpeng , Xu Xiaojun , Wang Shu , Gong Cuiping , Zhang Yuanqin , Zhu Pengcan , Gu Zhimin , Guan Wenzhi
Published: December 2024

Introduction

Largemouth bass is an economically important farmed freshwater fish species that has delicious meat, no intermuscular thorns, and rapid growth rates. However, the molecular regulatory mechanisms underlying the different growth and developmental stages of this fish have not been reported.

Methods

In this study, we performed histological and transcriptomic analyses on the brain and dorsal muscles of largemouth bass at different growth periods. The brain and muscle tissue were dehydrated, embedded, sliced and stained with hematoxylin-eosin. Images were captured under a microscope and acquired using a microphotographic system. Differential expression between groups was analyzed using DESeq2. GO functional analysis and KEGG pathway analysis were then performed for differentially expressed genes. RT-qPCR validates the reliability of transcriptome sequencing data.

Result

Smaller fish had more new muscle fiber numbers and wider intermuscular spaces compared to big specimens. Axons and nerve fibers were more pronounced in the telencephalons of big fish than in small fish. A total of 19,225 differentially expressed genes (DEGs) were detected in the muscle tissue, among which 7,724 were upregulated and 11,501 were downregulated, while a total of 5,373 DEGs were detected in the brain, among which 2,923 were upregulated and 2,450 were downregulated. GO and KEGG enrichment analyses indicated that nucleic acid binding, cytoskeletal motor activity, DNA binding, circadian rhythm, glycolysis/gluconeogenesis, and osteoclast differentiation were related to brain development while binding, cytoskeletal protein binding, biological processes, c-type lectin receptors, mitogen-activated protein kinase (MAPK) signaling pathways, and osteoclast differentiation were related to muscle growth. Stat3, pparg, akt1, mapk3, and mapk1 genes were mainly involved in the growth and development of largemouth bass.

Conclusion

These results provide novel perspectives for deepening our understanding of the mechanisms underlying the growth and development and performing genetic selection in largemouth bass.
Identification of biomarkers and immune microenvironment associated with pterygium through bioinformatics and machine learning
Authors: Zhang Li-Wei , Yang Ji , Jiang Hua-Wei , Yang Xiu-Qiang , Chen Ya-Nan , Ying Wei-Dang , Deng Ying-Liang , Zhang Min-hui , Liu Hai , Zhang Hong-Lei
Published: December 2024

Background

Pterygium is a complex ocular surface disease characterized by the abnormal proliferation and growth of conjunctival and fibrovascular tissues at the corneal-scleral margin. Understanding the underlying molecular mechanisms of pterygium is crucial for developing effective diagnostic and therapeutic strategies.

Methods

To elucidate the molecular mechanisms of pterygium, we conducted a differential gene expression analysis between pterygium and normal conjunctival tissues using high-throughput RNA sequencing. We identified differentially expressed genes (DEGs) with statistical significance (adjust p < 0.05, |logFC| > 1). Enrichment analyses were performed to assess the biological processes and signaling pathways associated with these DEGs. Additionally, we utilized weighted correlation network analysis (WGCNA) to select module genes and applied Random Forest (RF) and Support Vector Machine (SVM) algorithms to identify pivotal feature genes influencing pterygium progression. The diagnostic potential of these genes was validated using external datasets (GSE2513 and GSE51995). Immune cell infiltration analysis was conducted using CIBERSORT to compare immune cell populations between pterygium and normal conjunctival tissues. Quantitative PCR (qPCR) was used to confirm the expression levels of the identified feature genes. Furthermore, we identified key miRNAs and candidate drugs targeting these feature genes.

Results

A total of 718 DEGs were identified in pterygium tissues compared to normal conjunctival tissues, with 254 genes showing upregulated expression and 464 genes exhibiting downregulated expression. Enrichment analyses revealed that these DEGs were significantly associated with inflammatory processes and key signaling pathways, notably leukocyte migration and IL-17 signaling. Using WGCNA, RF, and SVM, we identified KRT10 and NGEF as pivotal feature genes influencing pterygium progression. The diagnostic potential of these genes was validated using external datasets. Immune cell infiltration analysis demonstrated significant differences in immune cell populations between pterygium and normal conjunctival tissues, with an increased presence of M1 macrophages and resting dendritic cells in pterygium samples. qPCR analysis confirmed the elevated expression of KRT10 and NGEF in pterygium tissues.

Conclusion

Our findings emphasize the importance of gene expression profiling in unraveling the pathogenesis of pterygium. The identification of pivotal feature gene KRT10 and NGEF provide valuable insights into the molecular mechanisms underlying pterygium progression.
Brain tumor-related epilepsy: an overview on neuropsychological, behavioral, and quality of life issues and assessment methodology
Authors: Maschio Marta , Perversi Fabio , Maialetti Andrea
Published: December 2024

Brain tumor-related epilepsy (BTRE) is a rare disease in which brain tumor (BT) and epilepsy overlap simultaneously and can have a negative impact on a patient’s neuropsychological, behavioral, and quality of life (QoL) spheres. In this review we (a) addressed the main neuropsychological, behavioral, and QoL issues that may occur in BTRE patients, (b) described how BT, BTRE, and their respective treatments can impact these domains, and (c) identified tools and standardized evaluation methodologies specific for BTRE patients. Neuropsychological disorders and behavioral issues can be direct consequences of BTRE and all related treatments, such as surgery, anti-cancer and anti-seizure medication, corticosteroids, etc., which can alter the structure of specific brain areas and networks, and by emotional aspects reactive to BTRE diagnosis, including the possible loss of autonomy, poor prognosis, and fear of death. Unfortunately, it seems there is a lack of uniformity in assessment methodologies, such as the administration of different batteries of neuropsychological tests, different times, frames, and purposes. Further research is needed to establish causality and deepen our understanding of the interplay between all these variables and our intervention in terms of diagnosis, treatment, psychosocial assessment, and their timing. We propose that the care of these patients to rely on the concepts of “BTRE-induced disability” and “biopsychosocial model” of BTRE, to prompt healthcare providers to handle and monitor BTRE-related psychological and social aspects, as to maintain the patient’s best possible QoL.
Impact of SARS-CoV-2 infection on patients with myasthenia gravis: a retrospective study in a Chinese population
Authors: Liu Peng , Li Mengna , Li Liqing , Jia Wenli , Dong Huimin , Qi Guoyan
Published: December 2024

Background and purpose

Myasthenia gravis (MG) is characterized by fluctuating muscle weakness due to immune-mediated damage to acetylcholine receptors. Viral infections can exacerbate symptoms of muscle weakness, and the clinical status of patients with MG may influence the outcomes of such infections. Here, we identified factors of symptom exacerbation, severe SARS-CoV-2 infection, and pneumonia in patients with MG who are infected with SARS-CoV-2.

Methods

The clinical characteristics and outcomes of 341 MG patients infected with SARS-CoV-2 across multiple regions in China were determined.

Results

The median age of the patients was 49 years (range: 35–60 years) and the median disease duration was 4 years (range: 2–8 years). Among the patients, 67 (49.0%) were male and 174 (51.0%) were female. Multivariate analysis indicated that thymectomy [OR, 1.654 (95% CI, 1.036–2.643); p = 0.035], severe SARS-CoV-2 infection [OR, 4.275 (95% CI, 2.206–8.286); p < 0.001], and pyridostigmine bromide [OR, 1.955 (95% CI, 1.192–3.206); p = 0.008] were associated with exacerbation of MG symptoms in patients infected with SARS-CoV-2. Age was significantly associated with severe SARS-CoV-2 infection [OR, 1.023 (95% CI, 1.001–1.046); p = 0.008], while patients with cardiac/vascular comorbidities exhibited an increased likelihood of severe SARS-CoV-2 infection [OR, 3.276 (95% CI, 1.027–10.449); p = 0.045]. Likewise, steroid treatment [OR, 6.140 (95% CI, 2.335–16.140); p < 0.001] was associated with a significantly increased likelihood of severe SARS-CoV-2 infection compared with symptomatic treatment. Additionally, gender [OR, 0.323 (95% CI, 0.120–0.868); p = 0.025] and SARS-CoV-2 severity [OR, 6.067 (95% CI, 1.953–18.850); p = 0.002] were associated with the occurrence of pneumonia.

Conclusion

We identified factors that were associated with the exacerbation of MG symptoms in patients infected with SARS-CoV-2, including thymectomy, severe SARS-CoV-2 infection, and the use of pyridostigmine bromide. Due to the retrospective nature of the study, these findings should be interpreted as associations rather than predictive factors. However, the results confirm the established relationships between severe SARS-CoV-2 infection and age, cardiovascular comorbidities, and the use of steroid treatment, suggesting that these factors should be considered when managing MG patients during SARS-CoV-2 infection.
Metabolic syndrome therapy in pediatric age — between classic and modern. From diets to pipeline drugs
Authors: Frăsinariu Otilia Elena , Lupu Vasile Valeriu , Trandafir Laura Mihaela , Streanga Violeta , Jechel Elena , Bararu-Bojan Iris , Vasiliu Ioana , Cuciureanu Magdalena , Loghin Isabela Ioana , Mitrofan Costica , Nedelcu Alin Horatiu , Knieling Anton , Lupu Ancuta
Published: December 2024

The metabolic syndrome, made up of the sum of the entities that define it (obesity, hypertension, dyslipidemias and non-alcoholic hepatic steatosis) has gained an important place in the research of the last decades. This aspect is mainly due to the complexity of management in pediatric practice. The main directions in his approach therefore bring together the concern of counteracting the noise or systemic, of the multiple intercurrents at the physiopathological level, as well as the negative imprint exerted on the quality of life. Its appearance and evolution are currently controversial topics, but the influence of genetic predisposition and lifestyle (diet, physical activity, psychological balance) are certainties. Considering the escalation of the incident at the global level, it is self-evident that it is necessary to know the pathogenesis and practice countermeasures for prophylactic or therapeutic purposes. The present work aims to summarize general aspects related to the metabolic syndrome encountered in pediatric age, with an emphasis on complementary therapeutic perspectives and their effectiveness, by analyzing the latest data from the specialized literature, accessed with the help of international databases (e.g., PubMed, Web of Science, Scopus, Embase, Google Scholar).
The number of involved regions by prostate adenocarcinoma predicts histopathology concordance between radical prostatectomy specimens and MRI/ultrasound-fusion targeted prostate biopsy
Authors: Yusim Igor , Mazor Elad , Frumkin Einat , Hefer Ben , Li Sveta , Novack Victor , Mabjeesh Nicola J.
Published: December 2024

Introduction

The prostate biopsy (PB) results should be concordant with prostatectomy histopathology to avoid overestimating or underestimating the disease, leading to inappropriate or undertreatment of prostate cancer (PCa) patients. Since the introduction of multiparametric Magnetic Resonance Imaging (mpMRI) in the diagnostic pathway of PCa, most studies have shown that MRI/Ultrasound fusion-guided (MRI-fusion) PB improves concordance with histopathology of radical prostatectomy specimens. This study aimed to evaluate the improvement in concordance of prostatectomy specimens with PB histopathology obtained using the MRI-fusion approach compared with the 12-core TRUS-Bx and to identify the variables influencing this.

Patients and methods

The study included 218 men who were diagnosed with PCa by PB and underwent radical prostatectomy between 2016 and 2023. The patients were grouped based on the biopsy method: 115 underwent TRUS-Bx, and 103 underwent MRI-fusion PB. The histopathological grading of these biopsy approaches was compared with that of radical prostatectomy specimens. Multivariate logistic regression analyses were conducted to evaluate the impact of various criteria on histopathological concordance.

Results

In patients with unfavorable intermediate- and high-risk PCa, MRI-fusion PB showed significantly better concordance with prostatectomy histopathology than TRUS-Bx (73.1% vs. 42.9%, p = 0.018). MRI-fusion PB had a significantly lower downgrading of prostatectomy histopathology than TRUS-Bx in all grade categories. The number of cancer-involved regions of the prostate is an independent predictor for concordance (OR = 1.24, 95%CI = 1.04-1.52, p = 0.02) and downgrading (OR = 0.46, 95%CI = 0.24-0.83, p = 0.01).

Conclusions

Using an MRI-fusion PB improves histopathological concordance in patients with unfavorable intermediate and high-risk PCa. It reduces the downgrading rate of prostatectomy histopathology compared with TRUS-Bx in all grade categories. The number of cancer-involved regions is an independent predictor of the concordance between biopsy and final histopathology after prostatectomy and post-prostatectomy histopathology downgrading. Our findings could assist in selecting PCa patients for AS and focal treatment based on the histopathology obtained from the MRI-fusion PB.
Executive functioning challenges of adolescents born extremely and very preterm
Authors: Lee Samantha J. , Woodward Lianne J. , Moor Stephanie , Austin Nicola C.
Published: December 2024

Background

Children born very preterm (VPT; <32 weeks) are at increased risk of executive functioning (EF) difficulties. But less is known about the nature and extent of these executive difficulties during late adolescence, particularly across multiple EF domains and in response to varying degrees of executive demand.

Methods

Using data from a prospective longitudinal study, this paper describes the EF profiles of 92 VPT and 68 full-term (FT) adolescents at age 17 years. Relations between gestational age (GA) and later EF performance, in addition to neonatal predictors, were examined.

Results

VPT-born adolescents performed less well than FT adolescents across the domains of working memory, planning, and cognitive flexibility, with the largest differences observed for those born <28 weeks GA (effect sizes −0.6 to −1.0 SD), and when task demands were high. The effects of GA on EF outcome were fully mediated by neonatal medical complexity (b = 0.169, t = −1.73) and term equivalent white matter abnormalities (b = 0.107, t = −3.33).

Conclusion

Findings support the need for long-term cognitive support for individuals born very preterm, particularly those exposed to high levels of medical and neurological risk, with these factors largely explaining associations between GA and EF outcome.
Mindfulness acting with awareness and emotional eating among polycystic ovary syndrome women with infertility: the mediating role of depression
Authors: Yang Mengye , Wang Xiaoyu , Zhang Yan , Qian Weina , Tang Yan
Published: December 2024

Emotional eating, characterized by the tendency to increase food intake in response to negative emotional states, is often linked to poor emotion regulation. While mindfulness-based interventions have been studied for their benefits in reducing emotional eating, less is known about how inherent mindfulness traits, relate to emotional regulation particularly among individuals with polycystic ovary syndrome (PCOS), a population known for high rates of psychological distress and disordered eating behaviors. This study investigates the associations between different facets of mindfulness, depressive symptoms, and emotional eating among individuals with PCOS and infertility who had not received any formal mindfulness intervention. A cross-sectional study was conducted involving 334 individuals. Participants completed the Five Facet Mindfulness Questionnaire-Short Form (FFMQ-SF), the Patient Health Questionnaire (PHQ-9) to assess depressive symptoms, and the Dutch Eating Behavior Questionnaire (DEBQ) to measure emotional eating. Structural Equation Modeling (SEM) was employed to examine the relationships between inherent mindfulness traits (i.e., observation, describing, acting with awareness, non-reactivity, and non-judgment), depressive symptoms, anxiety, and emotional eating. The findings indicated that the trait of acting with awareness may reduce emotional eating through its influence on depressive symptoms, while observational mindfulness was found to be associated with increased emotional eating without prior external mindfulness training. In conclusion, Mindfulness is a multidimensional construct, with its facets contributing differently to emotional regulation and eating behaviors in individuals with PCOS and infertility. Future research should explore these nuances to develop more targeted interventions.
Adolescent short video addiction in China: unveiling key growth stages and driving factors behind behavioral patterns
Authors: Guo Jiaxiang , Chai Ran
Published: December 2024

The proliferation of short video apps has brought to the forefront the issue of adolescent addiction, a challenge that resonates across Chinese society. Despite growing attention, a comprehensive understanding of the factors propelling addiction at various adolescent stages and its impact on academic performance remains scarce. This study aims to fill this void by identifying key growth stages and crafting targeted intervention strategies. Our investigation engaged 1,896 Chinese students, averaging 15 years old, at pivotal educational junctures. Employing a mixed-method approach, we utilized interviews and surveys, enhanced by fixed effects models and instrumental variables, to discern patterns in short video addiction. The study revealed stage-specific catalysts for addiction: social identity in junior high, academic stress in senior high, and personality traits in university students. A concerning trend was the double and triple rate of severe addiction among senior high students compared to their junior high and university counterparts, respectively, with a peak of 52.7% mild addiction in university. Our predictive model provides a holistic perspective on the drivers of addiction. This groundbreaking analysis delineates the complex interplay of adolescent short video addiction in China, under-scoring its profound implications for academic progression in senior high. The findings under-score the urgent need for tailored interventions to counteract the adverse effects of addiction.
Latent class analysis for health-related quality of life in nurses in China
Authors: Zhao Yan , Yang Bei , Chu Jianying
Published: December 2024

Background

This study aimed to identify the types of quality of life (QoL) based on the five dimensions of the EQ-5D and predict factors affecting QoL.

Methods

A multistage stratified cluster sampling survey was conducted among the staff of 12 general hospitals, 1,965 nurses completed the survey, and the data were analyzed using SPSS 26.0 and Mplus 8.3 for latent analysis.

Results

Three latent classes of QoL were identified: low-level (2.8%), pain and discomfort (7.6%), medium-level (47.1%), and high-level (42.5%). The types and characteristics of QoL differed among these latent classes. The low-level group had the lowest EQ visual analog scale (EQ-VAS) score (F = 75.217, P < 0.001) and the highest K10 score (F = 61.90, P < 0.001). Moreover, increased age (OR = 0.819, 95% CI: 0.817–0.973), never having drunk alcohol (OR = 0.107, 95% CI: 0.023, 0.488), and increased EQ-VAS scores (OR = 0.935, 95% CI: 0.919, 0.952) were protective factors for quality of life, while working in obstetrics and gynecology (OR = 6.457, 95% CI:1.852, 22.512) and higher K10 scores (OR = 1.153, 95% CI: 1.100, 1.209) were risk factors for quality of life.

Conclusion

The results indicated significant heterogeneity in the types of QoL and identified predictors of QoL. These findings provide basic information for the development of nursing interventions to improve quality of life and identified specific characteristics that should be considered during intervention development.