bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengembangan Media E-Book Cerita Fiksi Untuk Meningkatkan Keterampilan Menulis Siswa Kelas VI Sekolah Dasar
Authors: Liyawindari Anis Eko , Sukartiningsih Wahyu , Suparti Suparti
Published: May 2023

Penelitian pengembangan ini bertujuan 1) mendiskripsikan validitas pengembangan media E-Book cerita fiksi, 2) mendeskripsikan kepraktisan media E-Book cerita fiksi ditinjau materi dan penyajian materi, 3) dan mendeskripsikan keefektifan media E-Book cerita fiksi untuk meningkatkan keterampilan menulis cerita fiksi siswa kelas VI SD . Metode yang digunakan dalam penelitian ini menggunakan penelitian R&D model ADDIE dengan menggunakan 5 tahap yaitu analysis, design, development, implementation, evaluation. Validitas diperoleh dari hasil validasi oleh ahli media dengan validitas media sebesar 92% dan 89%, materi , dan ahli bahasa yang dianalisis menggunakan metode deskriptif kuantitatif. Hasil analisis menunjukkan bahwa E-Book cerita fiksi yang dikembangkan telah layak dari penilaian yang dilakukan pada ahli media sebesar 92% dan 89%, materi 91% dan 93% dan ahli Bahasa dengan menunjukkan hasil sebesar 92%, 89%, dan 92%  yang termasuk pada kategori “sangat valid” ahli. Sementara itu pada pengujian keefektifan media e-book media E-Book cerita fiksi dengan menggunakan uji-t pada kelompok kecil diperoleh bahwa nilai sig (2-tailed) sebesar 0,000 mendapatkan nilai tersebut kurang dari 0,05 sedangkan pada uji kelompok besar  juga diperoleh bahwa nilai sig (2-tailed) sebesar 0,000 sehingga dapat disimpulkan bahwa media e-book ini telah layak dan efektif dalam meningkatkan keterampilan menulis cerita fiksi siswa kelas VI sekolah dasar.
Applications of a powerful model organism Caenorhabditis elegans to study the neurotoxicity induced by heavy metals and pesticides
Authors: Melnikov K , Kucharíková S , Bárdyová Z , Botek N , Kaiglová A
Published: May 2023

The expansion of industry and the use of pesticides in agriculture represent one of the major causes of environmental contamination. Unfortunately, individuals and animals are exposed to these foreign and often toxic substances on a daily basis. Therefore, it is crucial to monitor the impact of such chemicals on human health. Several in vitro studies have addressed this issue, but it is difficult to explore the impact of these compounds on living organisms. A nematode Caenorhabditis elegans has become a useful alternative to animal models mainly because of its transparent body, fast growth, short life cycle, and easy cultivation. Furthermore, at the molecular level, there are significant similarities between humans and C. elegans. These unique features make it an excellent model to complement mammalian models in toxicology research. Heavy metals and pesticides, which are considered environmental contaminants, are known to have affected the locomotion, feeding behavior, brood size, growth, life span, and cell death of C. elegans. Today, there are increasing numbers of research articles dedicated to this topic, of which we summarized the most recent findings dedicated to the effect of heavy metals, heavy metal mixtures, and pesticides on the well–characterized nervous system of this nematode.
PENDEKATAN ACTIVE LEARNING UNTUK MENINGKATKAN MOTIVASI BELAJAR PADA PEMBELAJARAN TEMATIK SEKOLAH DASAR KUTAGANDOK 2
Authors: Khoirunisa Ananda Salsabila , Sulastri Tuti , Pujihastuti Isti
Published: May 2023

Successful education will create appropriate and decent human beings in society and will not trouble others. The active learning method is a perspective that considers learning as an activity to build meaning or understanding of experiences and information carried out by the learner, not by the teacher. Motivation is a force or factor contained in humans, which causes, directs, and organizes behavior. The participants of the training for learning motivation and increasing enthusiasm are the 5th grade students of SDN Kutagandok 2 which consists of 31 students. It is hoped that the children will have the motivation and skills to complete various tasks, both school assignments and other tasks. Children get additional insight so that they can change their mindset and have the resilience according to their age growth.
PELATIHAN PERAWATAN KENDARAAN RODA DUA BAGI MASYARAKAT DI DUSUN CITEUREUP 1
Authors: Mutaqien Ibnu , Putri Anisa , Fajarwati Diana
Published: May 2023

Motorcycles are a means of transportation that are often used in daily activities, increasingly sophisticated technological developments have begun to be combined with the help of electronic control circuits such as an injection system to control combustion in the motorcycle combustion chamber or indicators to show a hot engine temperature that makes it easier for motorcycle owners to maintain the vehicle. Therefore, because of the lack of workshops or vehicle service services here the author will share information about motorcycle maintenance that can be done alone to save costs and can be developed as a form of business, such as a workshop. With the lack of information, most people only use motorbikes for their daily needs and ignore motorbike maintenance. Here the author will educate the community on how to take care of their two-wheeled vehicles in the citeureup 1 hamlet with methods of socializing the importance of caring for two-wheeled vehicles, training on caring for two-wheeled vehicles, and evaluating to ensure that people can take care of their own motorbikes. This program also has the aim that the people of Citeureup 1 Hamlet, Kutamukti Village, Kutawaluya District, Karawang Regency are able to maintain their own motorcycles and can be developed as a form of business, namely workshops.
MENINGKATKAN KETERAMPILAN SOSIAL ANAK MELALUI PERMAINAN TRADISIONAL DI DUSUN CITEUREUP II
Authors: Khulsum Hani , Rahmanto Ikhwan , Supratno Setyo
Published: May 2023

This study aims to provide data and information about efforts to improve children's social skills through traditional game playing methods in Citeureup II Hamlet, Kutamukti, Karawang. The research method used is the community education method, which consists of sharing section activities as an initial action, then actions in the form of playing traditional games with children and ending with a sharing section again as a form of reflection to find out the development of social skills in children. Data collection techniques use questionnaires (pre-test and post-test), interviews and observations. The sample in this study consisted of 20 children aged 10-12 years. The results of the pre-test showed that the total score obtained was 1049. After the implementation of the community education method through traditional games which was carried out for 5 days, it resulted in a pos-test result score of 1482 or there was an increase in the score of 447 from the total score of the previous pretest.  shows that traditional games are effective in improving children's social skills.
PELATIHAN PRODUKSI TELUR ASIN ASAP SEBAGAI UPAYA MENINGKATKAN NILAI JUAL TELUR BEBEK
Authors: Aulika Liza , Cahyati Ari Dewi , Rofieq Ainur
Published: May 2023

Pulo Kaum is one of the remote hamlets within the administrative scope of Kutagandok Village, Kutawaluya District, Karawang Regency, West Java. Unexpectedly, it turns out that this remote village in the middle of a hug rice field, there is potential of all fields. there are several duck breeders who produce boiled salted eggs and are produced in a traditional way with the amount of production not being too large. Since marketing is limited due to many similar industries in a single village as well as in a sub-region, almost every sub-region also has salted egg production communities. because duck eggs have a relatively cheap selling value here, it is necessary to do smoking to increase the selling value of the duck eggs and of course this process can also help the duck eggs to be durable. The purpose of this activity is to increase the selling value of duck eggs, and of course this process can also contribute to the durability of ducks eggs. The aim of this activity is to increase the sales value of duck eggs and to produce sustainable duck eggs using the smoking method. The operation will take place from August to September 2022 in Pulo Kaum Hamlet, Kutagandok Village, Kutawaluya District, Karawang Regency, West Java. The method of carrying out the activity consists of counseling, training and mentoring the residents of Pulo Kaum Hamlet, especially the housewives. The result of this program is an increase in sales value and income from the sale of smoked salted eggs, as well as the creation of smoked salted eggs with a longer shelf life. The conclusion of this program is that producing more and more duck eggs can further increase profits.
PELATIHAN DIGITAL MARKETING BAGI PELAKU UMKM KUE TRADISIONAL DUSUN JUNTI KAUM
Authors: Candra Eka , Suryani Elvira , Putra Purnama
Published: May 2023

The internet has been used by various circles of society, including Micro, small and medium Enterprises entrepreneurs in the regions. However, the lack of insight and education for Micro, Small and Medium Enterprises entrepreneurs causes not optimal use of the internet in supporting business processes in order to increase the profits achieved by Micro, Small and Medium Enterprises. One of the uses of the internet in business management is digital marketing. Digital Marketing is a form of marketing or promotion of a product using digital media or the internet with the aim of attracting consumers quickly. Based on the results of observations and interviews conducted, it was found that there were problems where MSME actors were still selling products manually and had not done marketing through digital marketing. The implementation method used is training with the aim of increasing the understanding of Micro, Small and Medium Enterprises of Traditional Cakes about digital marketing in Junti Kaum Hamlet. The activities include providing counseling on digital marketing to Micro, Small and Medium Enterprises, training on making digital stores and training in marketing publications. This activity is carried out as a form of community service through Real Work Lectures organized by the 45 Bekasi Islamic University Research and Service Institute.
PELATIHAN PEMASARAN PRODUK DARI LIMBAH SEKAM PADI MENGGUNAKAN DIGITAL MARKETING DI DUSUN BEDENG
Authors: Putri Duwi Rahma , Tama Annafi Indra , Mahmudah Hadi
Published: May 2023

The main problem that occurs is the low quality of human resources in mastering technology and the lack of public understanding in terms of marketing. To overcome this problem, it is necessary to conduct marketing training using digital marketing. The purpose of implementing digital marketing training is to provide an understanding related to the importance of conventional and digital marketing. The implementation method used is through training which consists of the socialization stage about digital marketing, logos and labels, the training stage on how to operate the social media platform, and the evaluation stage of the activities that have been carried out. The results of the implementation of the community can distinguish between conventional marketing and digital marketing, understand how to create logos and labels on marketing and create WhatsApp Business and Instagram accounts as digital marketing. These two platforms were chosen because they are easy for the public to understand. However, the constraint of this training is the limited number of people who have smartphones.
PENGELOLAAN SAMPAH MELALUI PEMILAHAN DAN PEMANFAATAN SAMPAH PLASTIK SERTA KULIT BUAH SEBAGAI BAHAN ECO-ENZYME
Authors: Yasid Syifa Fauziah , Pramono Joko , Rahmawati Rafika
Published: May 2023

The problem of waste is familiar to hear, especially household waste. This can be seen in one of the villages in Karawang Regency, named Kutamukti Village, especially in Poris Village. Poris Village is the second village with the highest population. Surely this results in a lot of waste that will be generated from daily activities including household activities. Waste Management in Poris Village is still very worrying because the majority of people still dispose of household waste in the river or burned. This is due to the lack of public understanding of the management and utilization of household waste. By providing socialization, practice, and counseling related to waste management and utilization, the community will understand and realize that waste greatly affects the health of the environment and the health of the people around it. The existence of these activities, the community will be able to manage household waste by sorting waste 3R (Reduce, Reuse, and Recycle) and use it to plant from plastic bottles and as a material for making eco-enzyme. That way will make the surrounding environment clean, comfortable, and the quality of the environment is maintained.
PENGUATAN PERUBAHAN PERILAKU DALAM PERCEPATAN PENURUNAN STUNTING
Authors: Nurhidayah Siti , Basri Hasan , Ridwan Ridwan , Suarlan Irlan
Published: May 2023

The implementation of community service carried out in Karawang Regency related to strengthening behavior change in accelerating stunting reduction is carried out with three activities, namely holding stunting prevention is important, stunting prevention socialization through bilingual pamphlets and outreach to pregnant women. The service implementation applies the community education method in the form of socialization. Community service is carried out within a month with locations in three hamlets, namely Kaliasin 2 Hamlet, Karang Mulya Hamlet and East Junta Hamlet. The results of the implementation of the service show that parents, especially mothers and prospective mothers, know and understand ways and steps to prevent and minimize stunting, increase community enthusiasm to maintain a healthy lifestyle as well as good parenting, increase awareness to participate in stunting programs and know how to prevent delays growth of toddlers, preventing children from being susceptible to infectious diseases, preventing poor performance in their learning focus and memory abilities.
PRODUK KEWIRAUSAHAAN DARI LIMBAH BIJI RAMBUTAN DESA SUKOSARI DUSUN PULOSARI KASEMBON
Authors: Lailiyah Shinta Nuriyatul , Adityo Rayno Dwi , Ali Salmannisa Najah , Salsabila Zahara , Firdaus Mohammad Tegar , Marzuki Rahmatullah Alaik
Published: May 2023

The need for food in human life affects food sources that continue to increase. As one of the efforts that can be made by utilizing local potential that can be processed into food products for the abundance of existing potential. Judging from the local potential of Dusun Pulosari Kasembon East Java in the form of rambutan plants planted in the yard of the house as a shade tree. During the rambutan season, residents only use the fruit for personal consumption and sale. Meanwhile, rambutan seeds (Nephelium sp) can be used as side products, such as the popular rambutan seed chips and nuts. The method used in this activity was in the form of training (socialization) which was carried out as part of Group 115 KKM activities which were primarily aimed at the people of Pulosari Hamlet, Kasembon District. The purpose of this activity is as a form of education and training for residents in processing rambutan seeds into chips and rambutan seed nuts which will later be developed and sold to the general public. The result of this training is to provide knowledge and convenience for residents to utilize rambutan seeds which have not been used so far so that they become a commodity of economic value.
PELATIHAN PENENTUAN ARAH KIBLAT BAGI SANTRI PONDOK PESANTREN HIDAYATUL QUR’AN (PPHQ)
Authors: Ardliansyah Moelki Fahmi , Sakirman Sakirman , Jamil Jamil
Published: May 2023

Astronomy in Indonesia originates and develops from Islamic boarding schools. Currently, this science is not massively studied in Islamic boarding schools, because it is considered difficult to do something related to calculations. Whereas in the aspect of study in Fiqh, students are taught and given an in-depth explanation. The Hidayatul Quran Islamic Boarding School (PPHQ) in Batanghari, East Lampung, is no exception, which has two flagship programs, namely Diniyah Takmiliyah and Tahfidhul Qur'an. In the Diniyah Takmiliyah Program, where alfiyah class students have studied fiqh studies quite in depth, the study of istiqbalul qiblah fiqh is not overlooked. This service tries to complete the fiqh understanding of the Qibla that has been owned by the santri with a scientific understanding of the Qibla, both in terms of calculation and measurement. The method used is PAR (participatory action research) with the aim of seeing, hearing, as well as understanding social phenomena that occur. Through this method, the target community or group is used as a subject, not an object of activity. The results obtained were that the alfiyah class students had basic knowledge both in the field of fiqh and about the geography of the earth. So that this training can complement and add insight, knowledge, new understanding and new skills in determining the Qibla direction.
IMPLEMENTASI MODEL PEMBELAJARAN GENRE BASED APPROACH DALAM PENGAJARAN MENULIS TEKS IKLAN (ADVERTISEMENT) MELALUI LESSON STUDY
Authors: Hidayati Istiqlaliah Nurul , Rahmah Mursidah
Published: May 2023

Writing skill is one of the most important language skills to be mastered by students. There have been many learning models developed for writing especially in English but not all English teachers know and implement these learning models. One learning model that has been extensively researched and proven to be effective in teaching English writing skills is the Genre Based Approach. The English teacher's mastery of the Genre Based Approach learning model is very important because apart from being proven effective, it is also recommended by the government, especially in the implementation of the independent curriculum. It is projected for learning in the coming year, GBA will be required to be used as a model for learning English in schools, including in vocational schools. The service method is carried out using the lesson study model. Lesson study is carried out in three cycles until finally the students get a score that exceeds the KKM score. In addition to quantitative data in the form of increasing students' English writing scores, the volunteers also distributed questionnaires that asked students' perceptions after learning using the Genre Based Approach. From the answers to the questionnaire it can be concluded that GBA cognitively adds to their insights and this insight can be used for ideas in writing and affectively GBA makes them interested and challenged to write.
PENYULUHAN PERUBAHAN PEMAHAMAN DALAM PENCEGAHAN DAN PENURUNAN STUNTING DI KECAMATAN BATUJAYA KARAWANG
Authors: Nurhidayah Siti , Basri Hasan , Ridwan Ridwan , Putrianika Puput , Widyowati Dian Desty , Khoiriyah Ummi
Published: May 2023

Stunting is a nutritional problem in the world, especially in developing and poor countries. Stunting can increase the risk of morbidity and mortality, as well as suboptimal brain development resulting in stunted motor development and mental retardation. Karawang Regency is one of 100 areas in West Java that is prioritized for stunting prevention. Based on the latest data released by the Karawang Regency Health Office, the results of the August 2020 Toddler Month Weighing were as many as 155,670 toddlers whose length and height were measured, the results obtained were 688 toddlers with very low nutritional status (0.4%) and 3,720 short toddlers (2.4%) ). Meanwhile, the normal height was 150,875 toddlers (96.9%) and the height was 387 toddlers (0.2%). The cause of the high stunting rate in Karawang Regency is very complex. The determinants of stunting cases that have been collected by the Karawang District Health Office are the lack of clean water, healthy latrines, immunization, the number of pregnant women who experience a lack of protein energy, the number of family members who smoke, children who have worms and families who do not have health insurance. Based on the identification of the problem of stunting in Karawang Regency, stunting is not hereditary so it can be prevented. The main factor is changes in people's behavior. Therefore, the Institute for Research & Community Service (LPPM Unisma) carries out community service activities by making outreach activities for changes in understanding in accelerating stunting prevention in Batujaya District, Karawang Regency. Activities to increase behavior change in accelerating stunting prevention in Batujaya District, Karawang Regency are actually only carried out in the form of counseling and psychoeducation regarding changes in understanding in accelerating stunting prevention by involving various parties so that they can have a positive impact. has an impact on preventing and reducing stunting, as well as providing basic food packages for vulnerable families.
Comportamiento epidemiológico de la mortalidad perinatal y neonatal tardía, Colombia, 2019
Authors: Forero Motta Diana Alexa
Published: May 2023

Introducción: la mortalidad perinatal y neonatal tardía es un reflejo del acceso, la cobertura y la calidad del sistema de salud. Esta es influenciada por determinantes sociales como la pobreza, la desigualdad y la educación, entre otros. Materiales y métodos: estudio descriptivo retrospectivo con base en la notificación del evento individual al Sistema de Vigilancia en Salud Pública (Sivigila) durante el 2019. Resultados: el comportamiento del evento desde 2008 tiene una tendencia al descenso, para una razón de mortalidad en 2019 de 15,2 por 1 000 nacidos vivos, con un cambio porcentual anual de -0,93 (IC 95 % -1,5 - -0,3; p:0,0) lo que representa un descenso estadísticamente significativo. Las cinco entidades territoriales con el indicador más alto fueron Chocó (40,2); Vaupés (33,7); Buenaventura (32,1); Vichada (28,3) y Amazonas (23,7). Las razones de mortalidad más altas se encontraron en: 1) hijos de madres no aseguradas al sistema de salud, 2) hijos de madres con edades extremas (menores de 15 y mayores de 35 años) y 3) pertenencia étnica indígena. Los mayores problemas identificados son fallas de los programas de protección específica, detección temprana y demanda inducida, subestimación de la gravedad del paciente, fallas de calidad en los registros médicos e inoportunidad en la remisión. Conclusión: la mortalidad perinatal y neonatal tardía se relaciona con factores maternos, obstétricos, feto-neonatales, por lo cual es necesario continuar el fortalecimiento de la atención preconcepcional, prenatal, del parto y del postparto, así como disminuir las brechas sociales con el fin de mejorar la calidad de vida de la población maternoperinatal.
Investigación de brote de COVID-19 en repatriados desde Estados Unidos a Cundinamarca, Colombia, abril 2020
Authors: Díaz Jorge Luis , Alarcón Ángela Patricia , Zein Rizo Victor , Velásquez Alejandra , Walteros Diana Marcela , Salas Helena Patricia , Prieto Franklyn Edwin
Published: May 2023

Introducción: con base en el aumento y circulación sostenida en países como Francia, España, Canadá, Estados Unidos de América, Brasil e Italia el gobierno de Colombia en articulación con otros estados decidió realizar vuelos humanitarios desde y hacia países con transmisión activa bajo procedimientos de control de riesgos y bioseguridad. El objetivo de este estudio fue describir un brote de COVID-19 en un grupo de connacionales repatriados desde Estados Unidos a Colombia quienes permanecieron en aislamiento preventivo en un municipio de Cundinamarca, en abril de 2020. Materiales y métodos: estudio descriptivo de un brote por COVID-19, enmarcado en la metodología propuesta por los CDC, a través de un análisis de frecuencias, donde se evaluaron 64 connacionales, contactos estrechos, reportes de muestras y las zonas de aislamiento. Resultados: se confirmaron 24 casos positivos para SARS-CoV-2, 23 en connacionales y un caso en el personal de logística. Se encontraron 111 contactos estrechos, con una tasa de ataque del 21,62 %. La edad promedio de las personas encargadas de logística fue de 32 años, con una DE 10,9 (mínima de 19 y máxima de 64 años) de los cuales el 70,2 % (33) eran hombres y el 29,78 % (14) sin antecedentes médicos de importancia. Conclusiones: la trasmisión del evento se relacionó con el no cumplimiento del distanciamiento social, uso adecuado de elementos de bioseguridad y uso de zonas comunes de forma permanente.
Análisis espacial de COVID-19 en población procedente del exterior en Colombia, marzo de 2020 - octubre de 2021
Authors: Velásquez Morales Alejandra , León Velasco Paola Elena , Alarcón Ángela Patricia , Walteros Diana Marcela
Published: May 2023

Introducción: El alto flujo migratorio de personas procedentes del exterior implica mayor riesgo de propagación de SARS CoV2 en el territorio nacional. En Colombia desde marzo de 2020 a 31 de octubre de 2021 se han confirmado 67 787 casos en población extranjera. El objetivo es caracterizar epidemiológicamente la población extranjera de SARS CoV2 en el Sistema de Vigilancia de Salud Pública (Sivigila) desde marzo de 2020 a octubre de 2021. Materiales y métodos: estudio transversal observacional, con fuente primaria de datos de Sivigila desde marzo 2020 a octubre de 2021. Se calcularon medidas de tendencia central y dispersión, media y desviación estándar de las variables cuantitativas y proporciones para variables cualitativas. Se realizó análisis univariado y bivariado, se estimó razón de momios entre antecedentes, comorbilidades, edad y sexo, considerando estadísticamente significativos los valores p <0,05. Se realizó análisis espacial para identificar entidades territoriales de riesgo y procedencia de los casos importados. Resultados: se identificaron 67 893 casos de COVID 19 de población extranjera captada por la variable nacionalidad en el Sivigila. El promedio de edad fue de 35,8 ±15,8 (Me=33), la edad mínima fue de 0 años y la máxima 114 años. La variabilidad fue heterogénea (CV=42,81 %). El sexo predominante fue el masculino con el 51,9 % (n=35 203), femenino con 48,1 % (n=32 690). La ciudad con más casos corresponde a Bogotá con el 49,8 % de los casos (n=33 868), seguido del departamento de Antioquia con el 19,73 % (n=13.398), Valle del Cauca con el 5,64 % (n=3827) y Norte de Santander con 3,89 % (n=2640), El distrito de Barranquilla con 3,09 % (n=2098). Conclusión: en la población extranjera el número de casos es mayor en hombres, con una edad entre 30 a 39 años y 20 a 29 años. Se identificó que las muertes tienen una asociación con las comorbilidades de VIH y ser fumador. Se recomienda continuar con el seguimiento de esta población con el fin de identificar características demográficas de mayor vulnerabilidad y áreas de mayor afluencia para focalizar acciones de mitigación y control.
Effectiveness of combined methods of treatment of atrophic scars of skin
Authors: Dolgikh S. Yu. , Gryazeva N. V.
Published: May 2023

Atrophic post-acne scars are severe manifestations of the post-acne symptom complex, which occur as a result of inflammation due to the loss of collagen fibers and subcutaneous fat during the healing of inflammatory acne elements. The incidence of post-acne scars has been little studied, but according to some authors, about 90 % of patients with severe and moderate acne suffer from them. The aim of the study was to study the effect of the combined use of IPL- and PRP-therapy, fractional microneedle radiofrequency therapy and PRP-therapy, as well as microneedling of autologous plasma with cells on the clinical symptoms of atrophic skin scars, taking into account the duration of the pathological process, based on the dynamics of the indicators of the Manchester Assessment Scale of Scar Deformations. Complex methods showed higher efficiency than the use of hardware techniques in relation to all clinical signs of atrophic scars.
Introduction
Authors: Offenbach Seth , Eichhorn Niels
Published: May 2023

H-Net's Past-President Seth Offenbach and Vice President of Research and Publications Niels Eichhorn introduce the inaugural volume of the Proceedings of the H-Net Teaching Conference.
Emotional Intelligence and Spiritual Intelligence on Student Learning Motivation
Authors: Stella Yunita , Binar Sri , Purwanto Ani Teguh , Octavianus Jonathan
Published: May 2023

The purpose of this study is to examine the influence of emotional intelligence and spiritual intelligence on students' learning motivation. The research method employed in this study is quantitative with multiple linear regression analysis. The research was conducted at Sekolah Tinggi Teologi Injili Indonesia Surabaya, and the findings indicate that emotional intelligence and spiritual intelligence have a significant effect on students' learning motivation, accounting for 61.5% of the variance. Specifically, emotional intelligence has a 70.6% effect on learning motivation, while spiritual intelligence has a 76.9% effect.

Research Contribution: This study highlights the importance of emotional intelligence and spiritual intelligence in influencing students' learning motivation at Sekolah Tinggi Teologi Injili Indonesia Surabaya. Therefore, educational institutions, particularly Sekolah Tinggi Teologi Injili Indonesia Surabaya, should focus on organizing student activities that promote the development of emotional and spiritual intelligence. Furthermore, educators should employ innovative teaching methods to enhance students' emotional and spiritual intelligence, ultimately leading to increased learning motivation among students.
Larval development of the blue crab Callinectes sapidus: effect of enriched rotifers as live food
Authors: Ospina-Salazar Gloria Helena , Miranda-Baeza Anselmo , Zea Sven
Published: May 2023

Understanding nutrition and its effect on growth is essential for developing diets in commercially important species. This study aimed to evaluate the larval development of Callinectes sapidus fed with rotifers (Brachionus plicatilis) enriched with Chlorella pyrenoidosa (Ch) as a main protein source and a lipid emulsion (Spr) rich in highly unsaturated fatty acids. The enriched compositions had seven ratios Ch/Spr (w/w): 100/0, 90/10, 80/20, 70/30, 60/40, 50/50, and 40/60. The enriched rotifers with each ratio were called diets. Lipids increased progressively between 100/0 and 40/60 (7.48 to 11.30 g 100 g-1), as well as energy (21.88 to 23.16 kJ g-1), but the protein level did not show a clear trend (63.93 and 67.50 g 100 g-1). The production response of blue crab larvae showed significant differences within diets (P < 0.05). The highest survival (29 to 34%) was obtained in the 50/50 and 40/60 treatments. In the 40/60, 50/50, and 60/40 diets, the larval cycle was shorter (P < 0.05), and the megalopa stage was reached between 49 and 52 days of culture. Metamorphosis to megalopa occurred between the fifth and eighth molts; its corresponding weight varied from 0.72 to 1.33 mg, the length varied from 2,632 to 2,926 µm, and Le Cren´s condition factor varied from 0.83 to 1.16. The most important factor in the production response was the diet lipid content, which was significantly correlated with the survival, weight, length, and condition factor of C. sapidus larvae. Findings of the present study can be applied in future efforts towards mass culture of blue crab larvae in the Caribbean and can also potentially be used to study other crustacean species with similar characteristics.
Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector
Authors: Wang Jinduo , Wang Shuo , Zhao Siyu , Sun Pengjie , Zhang Zhen , Xu Qingyang
Published: May 2023

Introduction: L-lysine is a bulk product. In industrial production using high-biomass fermentation, the high density of bacteria and the intensity of production require sufficient cellular respiratory metabolism for support. Conventional bioreactors often have difficulty meeting the oxygen supply conditions for this fermentation process, which is not conducive to improving the sugar-amino acid conversion rate. In this study, we designed and developed an oxygen-enhanced bioreactor to address this problem.

Methods: This bioreactor optimizes the aeration mix using an internal liquid flow guide and multiple propellers.

Results: Compared with a conventional bioreactor, it improved the kLa from 367.57 to 875.64 h-1, an increase of 238.22%. The results show that the oxygen supply capacity of the oxygen-enhanced bioreactor is better than that of the conventional bioreactor. Its oxygenating effect increased the dissolved oxygen in the middle and late stages of fermentation by an average of 20%. The increased viability of Corynebacterium glutamicum LS260 in the mid to late stages of growth resulted in a yield of 185.3 g/L of L-lysine, 74.57% conversion of lysine from glucose, and productivity of 2.57 g/L/h, an increase of 11.0%, 6.01%, and 8.2%, respectively, over a conventional bioreactor. Oxygen vectors can further improve the production performance of lysine strains by increasing the oxygen uptake capacity of microorganisms. We compared the effects of different oxygen vectors on the production of L-lysine from LS260 fermentation and concluded that n-dodecane was the most suitable. Bacterial growth was smoother under these conditions, with a 2.78% increase in bacterial volume, a 6.53% increase in lysine production, and a 5.83% increase in conversion. The different addition times of the oxygen vectors also affected the final yield and conversion, with the addition of oxygen vectors at 0 h, 8 h, 16 h, and 24 h of fermentation increasing the yield by 6.31%, 12.44%, 9.93%, and 7.39%, respectively, compared to fermentation without the addition of oxygen vectors. The conversion rates increased by 5.83%, 8.73%, 7.13%, and 6.13%, respectively. The best results were achieved by adding oxygen vehicles at the 8th hour of fermentation, with a lysine yield of 208.36 g/L and a conversion rate of 83.3%. In addition, n-dodecane significantly reduced the amount of foam produced during fermentation, which is beneficial for fermentation control and equipment.

Conclusion: The new oxygen-enhanced bioreactor improves oxygen transfer efficiency, and oxygen vectors enhance the ability of cells to take up oxygen, which effectively solves the problem of insufficient oxygen supply during lysine fermentation. This study provides a new bioreactor and production solution for lysine fermentation.
Injectable thermo-sensitive hydrogel loaded hollow copper sulfide nanoparticles for ROS burst in TME and effective tumor treatment
Authors: Ning Shipeng , Mo Jianlan , Huang Rong , Liu Benkun , Fu Bicheng , Ding Shuaijie , Yang Huawei , Cui Ying , Yao Lei
Published: May 2023

Introduction: Lung cancer the most prevalent cause of cancer-related deaths, and current therapies lack sufficient specificity and efficacy. This study developed an injectable thermosensitive hydrogel harboring hollow copper sulfide nanoparticles and β-lapachone (Lap) (CLH) for lung tumor treatment.

Methods: The hydrogel-encapsulated CLH system can remotely control the release of copper ions (Cu2+) and drugs using photothermal effects for non-invasive controlled-release drug delivery in tumor therapy. The released Cu2+ consumes the overexpressed GSH in TME and the generated Cu+ further exploits the TME characteristics to initiate nanocatalytic reactions for generating highly toxic hydroxyl radicals. In addition, in cancer cells overexpressing Nicotinamide adenine dinucleotide (phosphate): quinone oxidoreductase 1 (NQO1), Lap can catalyze the generation of hydrogen peroxide (H2O2) through futile redox cycles. H2O2 is further converted into highly toxic hydroxyl radicals via the Fenton-like reaction, leading to a burst of reactive oxygen species in TME, which further enhances the therapeutic effect of chemokines.

Results: Analysis of the antitumor efficacy in a subcutaneous A549 lung tumor model mice showed a significant delay in tumor growth and no systemic toxicity was detected.

Discussion: In conclusion, we have established a CLH nanodrug platform that enables efficient lung tumor therapy through combined photothermal/chemodynamic therapy (CDT) treatment and self-supplying H2O2 to achieve cascade catalysis, leading to explosive amplification of oxidative stress.
The role of timing in the development and evolution of the limb
Authors: Zhu Meng , Tabin Clifford J.
Published: May 2023

The term heterochrony was coined to describe changes in the timing of developmental processes relative to an ancestral state. Limb development is a well-suited system to address the contribution of heterochrony to morphological evolution. We illustrate how timing mechanisms have been used to establish the correct pattern of the limb and provide cases where natural variations in timing have led to changes in limb morphology.
The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases
Authors: Fernández-Barat Laia , Vázquez Burgos Nil , Alcaraz Victoria , Bueno-Freire Leticia , López-Aladid Ruben , Cabrera Roberto , Gabarrús Albert , Palomeque Andrea , Oscanoa Patricia , Ceccato Adrian , Motos Ana , Amaro Rosanel , Bernardi Thierry , Provot Christian , Soler-Comas Alba , Muñoz Laura , Vila Jordi , Torres Antoni
Published: May 2023

Introduction

Biofilm production is an important yet currently overlooked aspect of diagnostic microbiology that has implications for antimicrobial stewardship. In this study, we aimed to validate and identify additional applications of the BioFilm Ring Test® (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE).

Materials and methods

Sputa were collected from BE patients who had at least one PA positive culture in the previous year. We processed the sputa to isolate both mucoid and non-mucoid PA, and determined their susceptibility pattern, mucA gene status, and presence of ciprofloxacin mutations in QRDR genes. The Biofilm production index (BPI) was obtained at 5 and 24 hours. Biofilms were imaged using Gram staining.

Results

We collected 69 PA isolates, including 33 mucoid and 36 non-mucoid. A BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype with 64% sensitivity and 72% specificity.

Conclusion

Overall, our findings suggest that the fitness-cost associated with the mucoid phenotype or ciprofloxacin resistance is shown through a time-dependent BPI profile. The BRT has the potential to reveal biofilm features with clinical implications.
Role of angiogenic transdifferentiation in vascular recovery
Authors: Cooke John P. , Lai Li
Published: May 2023

Tissue repair requires the orchestration of multiple processes involving a multiplicity of cellular effectors, signaling pathways, and cell-cell communication. The regeneration of the vasculature is a critical process for tissue repair and involves angiogenesis, adult vasculogenesis, and often arteriogenesis, which processes enable recovery of perfusion to deliver oxygen and nutrients to the repair or rebuild of the tissue. Endothelial cells play a major role in angiogenesis, whereas circulating angiogenic cells (primarily of hematopoietic origin) participate in adult vasculogenesis, and monocytes/macrophages have a defining role in the vascular remodeling that is necessary for arteriogenesis. Tissue fibroblasts participate in tissue repair by proliferating and generating the extracellular matrix as the structural scaffold for tissue regeneration. Heretofore, fibroblasts were not generally believed to be involved in vascular regeneration. However, we provide new data indicating that fibroblasts may undergo angiogenic transdifferentiation, to directly expand the microvasculature. Transdifferentiation of fibroblasts to endothelial cells is initiated by inflammatory signaling which increases DNA accessibility and cellular plasticity. In the environment of under-perfused tissue, the activated fibroblasts with increased DNA accessibility can now respond to angiogenic cytokines, which provide the transcriptional direction to induce fibroblasts to become endothelial cells. Periphery artery disease (PAD) involves the dysregulation of vascular repair and inflammation. Understanding the relationship between inflammation, transdifferentiation, and vascular regeneration may lead to a new therapeutic approach to PAD.
Dietary diversity, sociality, and the evolution of ant gustation
Authors: Smith E. Jordan , Vizueta Joel , Younger Meg A. , Mullen Sean P. , Traniello James F. A.
Published: May 2023

Ants have remarkably diverse diets and extraordinary species richness, making them an excellent model system to study the evolution of taste. In this entirely eusocial clade, food choice and the mechanisms that regulate feeding have both individual and social dimensions. How taste receptors and sensory processing drive food preferences to generate dietary breadth in ants is poorly understood. It is additionally unclear how elements of colony organization such as division of labor and social food flow impact the mechanistic basis and evolution of taste. Previous work on dipteran, lepidopteran, and hymenopteran gustatory systems, while foundational, provide limited insights into ant dietary specialization. Here we synthesize and analyze research on ant gustation to identify mechanisms, sociobiological correlates, and phylogenetic patterns. We discuss the current state of genomic analyses of taste and future research. We propose that strikingly polymorphic species of Pheidole, Cephalotes, Camponotus, and leafcutter ants (Atta and Acromyrmex) offer compelling social systems to explore adaptive variation in gustation because of their pronounced division of labor in which morphologically, behaviorally, and neurally differentiated workers vary in feeding behavior. Research on ant gustation within and among species will advance our understanding of sensory systems and provide insight into the impact of taste on the evolution of species diversity and how social organization influences gustation.
Assessing the financial viability of growing industrial hemp with loblolly pine plantations in the southeastern United States
Authors: Barnes Thomas , Parajuli Rajan , Leggett Zakiya , Suchoff David
Published: May 2023

Industrial hemp (Cannabis sativa L. <0.3% THC), a non-psychoactive chemotype of cannabis, was reclassified and made legal for growing across the United States under the 2018 Farm Bill. Given that resources, knowledge, and interest for this novel crop are expanding rapidly, we explored the possibility of intercropping industrial hemp for fiber with loblolly pine (Pinus taeda) plantations, one of the most commercially widespread tree species in the southern United States. Following a previous greenhouse study confirming hemp’s ability to grow in pine-influenced soils, we examined the financial feasibility of this potential agroforestry system. We simulated the loblolly pine tree growth information using PTAEDA 4.0, a growth and yield model, and collected the enterprise budget data on hemp productivity, operating and fixed costs, and prices from various sources. Based on the capital budgeting analyses, results suggest that pine-hemp intercropping can yield higher economic returns –at least 25% higher net present value—than the conventional monoculture loblolly pine plantation. The early rotation cash flow and the complimentary benefits can result in a more financially viable loblolly pine plantation under the intercropping scenario. While new research continues to advance further with field trials and other analyses, this study provides valuable insights into the current market conditions and productivity level of industrial hemp cultivation that need to be addressed for hemp intercropping to succeed as an economically viable agroforestry investment.
Innate immunity and early liver inflammation
Authors: Yang Zhou Jordi
Published: May 2023

The innate system constitutes a first-line defence mechanism against pathogens. 80% of the blood supply entering the human liver arrives from the splanchnic circulation through the portal vein, so it is constantly exposed to immunologically active substances and pathogens from the gastrointestinal tract. Rapid neutralization of pathogens and toxins is an essential function of the liver, but so too is avoidance of harmful and unnecessary immune reactions. This delicate balance of reactivity and tolerance is orchestrated by a diverse repertoire of hepatic immune cells. In particular, the human liver is enriched in many innate immune cell subsets, including Kupffer cells (KCs), innate lymphoid cells (ILCs) like Natural Killer (NK) cells and ILC-like unconventional T cells – namely Natural Killer T cells (NKT), γδ T cells and Mucosal-associated Invariant T cells (MAIT). These cells reside in the liver in a memory-effector state, so they respond quickly to trigger appropriate responses. The contribution of aberrant innate immunity to inflammatory liver diseases is now being better understood. In particular, we are beginning to understand how specific innate immune subsets trigger chronic liver inflammation, which ultimately results in hepatic fibrosis. In this review, we consider the roles of specific innate immune cell subsets in early inflammation in human liver disease.
Basophils beyond allergic and parasitic diseases
Authors: Poto Remo , Loffredo Stefania , Marone Gianni , Di Salvatore Antonio , de Paulis Amato , Schroeder John T. , Varricchi Gilda
Published: May 2023

Basophils bind IgE via FcεRI-αβγ2, which they uniquely share only with mast cells. In doing so, they can rapidly release mediators that are hallmark of allergic disease. This fundamental similarity, along with some morphological features shared by the two cell types, has long brought into question the biological significance that basophils mediate beyond that of mast cells. Unlike mast cells, which mature and reside in tissues, basophils are released into circulation from the bone marrow (constituting 1% of leukocytes), only to infiltrate tissues under specific inflammatory conditions. Evidence is emerging that basophils mediate non-redundant roles in allergic disease and, unsuspectingly, are implicated in a variety of other pathologies [e.g., myocardial infarction, autoimmunity, chronic obstructive pulmonary disease, fibrosis, cancer, etc.]. Recent findings strengthen the notion that these cells mediate protection from parasitic infections, whereas related studies implicate basophils promoting wound healing. Central to these functions is the substantial evidence that human and mouse basophils are increasingly implicated as important sources of IL-4 and IL-13. Nonetheless, much remains unclear regarding the role of basophils in pathology vs. homeostasis. In this review, we discuss the dichotomous (protective and/or harmful) roles of basophils in a wide spectrum of non-allergic disorders.