bluuebunny/crossref_metadata_2025 huggingface dataset:
The Luminous Fungi of Japan
Authors: Oba Yuichi , Hosaka Kentaro
Published: May 2023

Luminous fungi have long attracted public attention in Japan, from old folklore and fiction to current tourism, children’s toys, games, and picture books. At present, 25 species of luminous fungi have been discovered in Japan, which correspond to approximately one-fourth of the globally recognized species. This species richness is arguably due to the abundant presence of mycophiles looking to find new mushroom species and a tradition of night-time activities, such as firefly watching, in Japan. Bioluminescence, a field of bioscience focused on luminous organisms, has long been studied by many Japanese researchers, including the biochemistry and chemistry of luminous fungi. A Japanese Nobel Prize winner, Osamu Shimomura (1928–2018), primarily focused on the bioluminescence system of luminous fungi in the latter part of his life, and total elucidation of the mechanism was finally accomplished by an international research team with representatives from Russia, Brazil, and Japan in 2018. In this review, we focused on multiple aspects related to luminous fungi of Japan, including myth, taxonomy, and modern sciences.
Phenotypic Array for Identification and Screening of Antifungals against Aspergillus Isolates from Respiratory Infections in KwaZulu Natal, South Africa
Authors: Naicker Sarla , Mohanlall Viresh , Ngubane Sandile , Mellem John , Mchunu Nokuthula Peace
Published: May 2023

The rapid emergence of invasive fungal infections correlates with the increasing population of immunocompromised individuals, with many cases leading to death. The progressive increase in the incidence of Aspergillus isolates is even more severe due to the clinical challenges in treating invasive infections in immunocompromised patients with respiratory conditions. Rapid detection and diagnosis are needed to reduce mortality in individuals with invasive aspergillosis-related infections and thus efficient identification impacts clinical success. The phenotypic array method was compared to conventional morphology and molecular identification on thirty-six Aspergillus species isolated from patients with respiratory infections at the Inkosi Albert Luthuli Hospital in Kwa-Zulu Natal. In addition, an antimicrobial array was also carried out to screen for possible novel antimicrobial compounds for treatment. Although traditional morphological techniques are useful, genetic identification was the most reliable, assigning 26 to Aspergillus fumigatus species, 8 Aspergillus niger, and 2 Aspergillus flavus including cryptic species of A. niger, A. tubingensis and A. welwitschiae. The phenotypic array technique was only able to identify isolates up to the genus level due to a lack of adequate reference clinical species in the database. However, this technique proved crucial in assessing a wide range of possible antimicrobial options after these isolates exhibited some resistance to azoles. Antifungal profiles of the thirty-six isolates on the routine azole voriconazole showed a resistance of 6%, with 61% having moderate susceptibility. All isolates resistant to the salvage therapy drug, posaconazole pose a serious concern. Significantly, A. niger was the only species resistant (25%) to voriconazole and has recently been reported as the species isolated from patients with COVID-19-associated pulmonary aspergillosis (CAPA). Phenotypic microarray showed that 83% of the isolates were susceptible to the 24 new compounds and novel compounds were identified for potentially effective combination treatment of fungal infections. This study also reports the first TR34/98 mutation in Aspergillus clinical isolates which is located in the cyp51A gene.
New Species of Entoloma Subgenera Cubospora and Leptonia (Agaricales, Basidiomycota) from Central Vietnam
Authors: Morozova Olga , Pham Thi Ha Giang
Published: May 2023

Four new species of Entoloma from Kon Chu Rang Nature Reserve and Ta Dung National Park were discovered during an investigation of the diversity of the mycobiota of Central Vietnam and are described here on the base of the molecular and morphological data. Phylogenetic analysis was based on nrITS1-5.8S-ITS2, nrLSU and tef1α regions. Illustrated descriptions of their macro- and microscopic features and discussion on similar taxa are given. Entoloma cycneum and E. peristerinum belong to the subgenus Cubospora. They are morphologically similar species and are characterized by white or whitish basidiomata with yellowish or beige tinges and with mainly smooth, glabrous, and hygrophanous pileus, longitudinally fibrillose or fibrillose-scaly white stipe, cuboid spores, and more or less cylindrical cheilocystidia, arising from hymenophoral trama. Entoloma peristerinum posseses initially more coloured beige conical pileus, discolouring to white with age and drying. The pileus of E. cycneum is initially white, hemisphaerical to convex, usually with thin pubescence near the margin. The species can be recognized also by the cheilocystidia form: serrulatum-type in E. cycneum vs. porphyrogriseum-type in E. peristerinum. Another two species belong to the subgenus Leptonia. Entoloma tadungense is close to E. percoelestinum from which it differs by smaller spores with pronounced angles, presence of the cheilocystidia, and the lilac discolouration of the stipe. E. dichroides is named after its similarity to E. dichroum, a dark blue coloured species with pronouncedly angled basidiospores. It is distinguished by the basidiospores form—irregularly 5(–6) angled with elongated apiculus, as well as by absence of the cheilocystidia and darker basidiomata with conical pileus. The article also describes the history of the study of the genus Entoloma in Vietnam with a list of 29 species mentioned in the publications for this country.
Apical Vertebras Distribution Modifier for Coronal Balance Classification in Adult Idiopathic Scoliosis
Authors: Pan Aixing , Hai Yong , Lenke Lawrence G. , Zheng Zhaomin , Yang Jincai
Published: May 2023

Background: We aimed to propose the apical vertebras distribution modifier to supplement the coronal balance (CB) classification for adult idiopathic scoliosis (AdIS). An algorithm to predict postoperative coronal compensation and avoid postoperative coronal imbalance (CIB) was proposed. Methods: Patients were categorized into CB and CIB groups according to the preoperative coronal balance distance (CBD). The apical vertebras distribution modifier was defined as negative (−) if the centers of the apical vertebras (CoAVs) were on either side of the central sacral vertical line (CSVL) and positive (+) if the CoAVs were on the same side of the CSVL. Results: A total of 80 AdIS patients, with an average age of 25.97 ± 9.20 years, who underwent posterior spinal fusion (PSF) were prospectively recruited. The mean Cobb angle of the main curve was 107.25 ± 21.11 degrees at preoperation. The mean follow-up time was 3.76 ± 1.38 (2–8) years. At postoperation and follow-up, CIB occurred in 7 (70%) and 4 (40%) CB− patients, 23 (50%) and 13 (28.26%) CB+ patients, 6 (60%) and 6 (60%) CIB− patients, and 9 (64.29%) and 10 (71.43%) CIB+ patients. Health-related quality of life (HRQoL) was significantly better in the CIB− group compared with that of the CIB+ group in the dimension of back pain. To avoid postoperative CIB, the correction rate of the main curve (CRMC) should match the compensatory curve for CB−/+ patients; the CRMC should be greater than the compensatory curve for CIB− patients; and the CRMC should be less than the compensatory curve for CIB+ patients, and the inclination of the LIV needs to be reduced. Conclusions: CB+ patients have the least postoperative CIB rate and the best coronal compensatory ability. CIB+ patients are at a high risk of postoperative CIB and have the poorest coronal compensatory capacity in the event of postoperative CIB. The proposed surgical algorithm facilitates the handling of each type of coronal alignment.
Correlation Studies between S100 Protein Level and Soluble MIA or Tissue MelanA and gp100 (HMB45) Expression in Cutaneous Melanoma
Authors: Bolovan Lucica Madalina , Ceausu Mihai , Stanciu Adina Elena , Panait Marieta Elena , Busca Antonela , Hotnog Camelia Mia , Bleotu Coralia , Gales Laurentia Nicoleta , Georgescu Mihai Teodor , Prunoiu Virgiliu Mihail , Brasoveanu Lorelei Irina , Voinea Silviu Cristian
Published: May 2023

(1) Background: Cutaneous melanoma (CM) originates from melanocytes and causes 90% of skin cancer deaths; therefore, the comparison of different soluble and tissue markers could be valuable in the detection of melanoma progression and therapy monitoring. The present study is focused on the potential correlations between soluble S100B and MIA protein levels in different melanoma stages or with tissue expression of S100, gp100 (HMB45), and MelanA biomarkers. (2) Methods: Soluble S100B and MIA levels were evaluated by means of immunoassay methods in blood samples from 176 patients with CM, while tissue expressions of S100, MelanA, and gp100 (HMB45) were detected by means of immunohistochemistry in 76 melanomas. (3) Results: Soluble S100B correlated with MIA in stages III (r = 0.677, p < 0.001) and IV (r = 0.662, p < 0.001) but not in stages I and II; however, 22.22% and 31.98% of stage I and II patients, respectively, had high values for at least one of the two soluble markers. S100 tissue expression correlated with both MelanA (r = 0.610, p < 0.001) and HMB45 (r = 0.476, p < 0.01), while HMB45 and MelanA also significantly positively correlated (r = 0.623, p < 0.001). (4) Conclusions: Blood levels of S100B and MIA corroborated with melanoma tissue markers expression could help to improve the stratification process for patients with a high risk of tumor progression.
Organizational Aspects of the Implementation and Use of Whole Genome Sequencing and Whole Exome Sequencing in the Pediatric Population in Italy: Results of a Survey
Authors: Nurchis Mario Cesare , Raspolini Gian Marco , Heidar Alizadeh Aurora , Altamura Gerardo , Radio Francesca Clementina , Tartaglia Marco , Dallapiccola Bruno , Damiani Gianfranco
Published: May 2023

This study explores the organizational aspects of whole genome sequencing (WGS) implementation for pediatric patients with suspected genetic disorders in Italy, comparing it with whole exome sequencing (WES). Health professionals’ opinions were collected through an internet-based survey and analyzed using a qualitative summative content analysis methodology. Among the 16 respondents, most were clinical geneticists performing only WES, while 5 also used WGS. The key differences identified include higher needs for analyzing genome rearrangements following WES, greater data storage and security requirements for WGS, and WGS only being performed in specific research studies. No difference was detected in centralization and decentralization issues. The main cost factors included genetic consultations, library preparation and sequencing, bioinformatic analysis, interpretation and confirmation, data storage, and complementary diagnostic investigations. Both WES and WGS decreased the need for additional diagnostic analyses when not used as last-resort tests. Organizational aspects were similar for WGS and WES, but economic evidence gaps may exist for WGS in clinical settings. As sequencing costs decline, WGS will likely replace WES and traditional genetic testing. Tailored genomic policies and cost-effectiveness analyses are needed for WGS implementation in health systems. WGS shows promise for enhancing genetics knowledge and expediting diagnoses for pediatric patients with genetic disorders.
Prognostic Role of Clinical Features of Moderate Forms of COVID-19 Requiring Hospitalization
Authors: Trofor Antigona Carmen , Cernomaz Andrei Tudor , Lotrean Lucia Maria , Crișan-Dabija Radu Adrian , Penalvo Jose L. , Melinte Oana Elena , Popa Daniela Robu , Man Milena Adina
Published: May 2023

Introduction: We aimed to characterize the clinical features of moderate forms of COVID-19 requiring hospitalization and potentially identify predictors for unfavorable outcomes. Methods: Pooled anonymized clinical data from 452 COVID-19 patients hospitalized in two regional Romanian respiratory disease centers during the Alpha and Delta variant outbreaks were included in the analysis. Results: Cough and shortness of breath were the most common clinical features; older patients exhibited more fatigue and dyspnea and fewer upper airway-related symptoms such as smell loss or sore throat. The presence of confusion, shortness of breath and age over 60 years were significantly associated with worse outcomes (odds ratios 5.73, 2.08 and 3.29, respectively). Conclusion: The clinical picture on admission may have a prognostic role for moderate forms of COVID-19. Clear clinical definitions and developing adequate informational infrastructure allowing complex data sharing and analysis might be useful for fast research response should a similar outbreak occur in the future.
Association of Chronic Otitis Media with Sjogren’s Syndrome: A Case-Control Study
Authors: Yang Tzong-Hann , Chang Alison , Cheng Yen-Fu , Chen Chin-Shyan , Lin Herng-Ching
Published: May 2023

There is a paucity of large-scale population-based study whether patients with Sjögren’s syndrome are at higher risk of chronic otitis media. This study aimed to investigate the association of chronic otitis media with Sjögren’s syndrome by utilizing the representative dataset of the Taiwanese population. We identified 9473 patients with chronic otitis media as cases. We used propensity score matching to select 28,419 controls. We used multiple logistic regression analysis to examine the association of chronic otitis media with prior Sjögren’s syndrome after adjusting for age, sex, monthly income category, geographic location and urbanization level of the patient’s residence, allergic rhinitis, chronic rhinosinusitis and tonsillitis and adenoiditis. Chi-square tests showed a statistically significant difference in Sjögren’s syndrome between patients with chronic otitis media and controls (4.89% vs. 2.93%, p < 0.001). In addition, we found patients with chronic otitis media were more likely to have Sjögren’s syndrome (OR = 1.698, 95% CI = 1.509~1.910) relative to controls after adjusting for age, income, geographic location, residential urbanization level, allergic rhinitis, chronic rhinosinusitis and tonsillitis and adenoiditis. We also found that of the male patients, patients with chronic otitis media had a greater tendency to Sjögren’s syndrome than controls (adjusted OR = 1.982, 95% CI = 1.584~2.481). Similarly, a statistically significant association between Sjögren’s syndrome and chronic otitis media remains in female sampled patients (adjusted OR = 1.604, 95% CI = 1.396~1.842). We found that patients with Sjögren’s syndrome were associated with the occurrence of chronic otitis media. It may guide physicians as they counsel patients with Sjögren’s syndrome on the possibility of chronic otitis media occurrence.
Machine-Learning-Based Ground-Level Mobile Network Coverage Prediction Using UAV Measurements
Authors: Tarhuni Naser , Al Saadi Ibtihal , Asif Hafiz M. , Mesbah Mostefa , Eldirdiry Omer , Hossen Abdulnasir
Published: May 2023

Future mobile network operators and telecommunications authorities aim to provide reliable network coverage. Signal strength, normally assessed using standard drive tests over targeted areas, is an important factor strongly linked to user satisfaction. Drive tests are, however, time-consuming, expensive, and can be dangerous in hard-to-reach areas. An alternative safe method involves using drones or unmanned aerial vehicles (UAVs). The objective of this study was to use a drone to measure signal strength at discrete points a few meters above the ground and an artificial neural network (ANN) for processing the measured data and predicting signal strength at ground level. The drone was equipped with low-cost data logging equipment. The ANN was also used to classify specific ground locations in terms of signal coverage into poor, fair, good, and excellent. The data used in training and testing the ANN were collected by a measurement unit attached to a drone in different areas of Sultan Qaboos University campus in Muscat, Oman. A total of 12 locations with different topologies were scanned. The proposed method achieved an accuracy of 97% in predicting the ground level coverage based on measurements taken at higher altitudes. In addition, the performance of the ANN in predicting signal strength at ground level was evaluated using several test scenarios, achieving less than 3% mean square error (MSE). Additionally, data taken at different angles with respect to the vertical were also tested, and the prediction MSE was found to be less than approximately 3% for an angle of 68 degrees. Additionally, outdoor measurements were used to predict indoor coverage with an MSE of less than approximately 6%. Furthermore, in an attempt to find a globally accurate ANN module for the targeted area, all zones’ measurements were cross-tested on ANN modules trained for different zones. It was evaluated that, within the tested scenarios, an MSE of less than approximately 10% can be achieved with an ANN module trained on data from only one zone.
Coordinated PSO-ANFIS-Based 2 MPPT Control of Microgrid with Solar Photovoltaic and Battery Energy Storage System
Authors: SIddaraj Siddaraj , Yaragatti Udaykumar R. , Harischandrappa Nagendrappa
Published: May 2023

The microgrid is a group of smaller renewable energy sources (REs), which act in a coordinated manner to provide the required amount of active power and additional services when required. This article proposes coordinated power management for a microgrid with the integration of solar PV plants with maximum power point tracking (MPPT) to enhance power generation and conversion using a hybrid MPPT method based on particle swarm optimization-adaptive neuro-fuzzy inference system (PSO-ANFIS) to acquire rapid and maximum PV power along with battery energy storage control to maintain the stable voltage and frequency (V-f) of an isolated microgrid. In addition, it is proposed to provide active and reactive power (P-Q) regulation for the grid connected. The approach used provides more regulation due to the least root mean square error (RMSE), which improves photovoltaic (PV) potential extraction. The comparison results of the PSO-ANFIS and P&O controllers of the MPPT and the controller of the energy storage devices combined with the V-f (or P-Q) controller of the inverter all show effective coordination between the control systems. This is the most important need for contemporary microgrids, considering the potential of changing irradiance in the grid following mode, the grid forming mode under an island scenario, and back-to-grid synchronization. With the test model, the islanded and grid-islanded-grid connected modes are investigated separately. The results demonstrate conclusively that the proposed strategies are effective. To run the simulations, MATLAB and SimPowerSystems are utilized.
Facilitating Community Transition to Sustainable Land Governance: A Study of a Communal Settlement in South Africa
Authors: Pinfold Nicholas , Mokhele Masilonyane
Published: May 2023

Land is a fundamental resource that provides a foundation for the economy. Despite a wide range of studies on land governance systems, there is a lack of literature that analyzes the ability of communities to manage a change to different land governance systems. The study aimed to analyze the potential for the Goedverwacht communal settlement in the Western Cape province, South Africa, to transition from a hierarchical governance structure to one based on a communal land governance system. This aim was addressed by answering the research question: What are the roles, expectations and management strategies of the institutions and stakeholders participating in land governance? The study considered the community’s desire to maintain its communal settlement’s existence, and the choice between communal or individual freehold land governance. To understand these issues, the study utilized a framework that includes three theories: the theory of planned behaviour, the theory of institutional capacity, and the critical theory. (2) Methods: Through a survey, qualitative interviews, and focus group discussions, the study analyzed various underlying factors that influenced land governance and the land governance system desired by the community. (3) Results: The findings reveal that power dynamics and conflicting interests significantly affected the community’s ability to manage potential modernization resulting from land reform. While establishing land rights can positively impact economic growth and social mobilization, the lack of the communal settlement’s central government’s capacity to manage modernization effectively can lead to instability. (4) Conclusions: The paper concludes that balancing institutionalization and modernization is crucial for effectively managing the transition to new land governance systems.
Accessibility of Primary Schools in Rural Areas and the Impact of Topography: A Case Study in Nanjiang County, China
Authors: Zhu Yuanyuan , Zinda John Aloysius , Liu Qin , Wang Yukuan , Fu Bin , Li Ming
Published: May 2023

In recent years, many developing countries have consolidated rural primary schools, closed small community schools, and enlarged centralized schools, which can reduce the accessibility of education to many communities. Meanwhile, expanding road networks may enable people in far-flung communities to access schools more easily. To evaluate the impacts of both trends on spatial justice in access to education, it is important to examine spatial patterns of primary school accessibility and their predictors. How do the topographic features of villages and surrounding landscapes correlate with primary school accessibility in rural upland areas? Using a digital map route planning application, this study evaluates the primary school accessibility of each village in Nanjiang County, a mountainous county in southwest China. By evaluating relationships between primary school accessibility and village characteristics, this study provides evidence corroborating frequent claims that rural remote mountainous areas have poor primary school accessibility. Additionally, by analyzing the effects of elevation and ruggedness of villages and of the zone between villages and schools as well as the mechanisms driving these effects, we find that, contrary to expectations, with increasing village elevation, a village’s primary school accessibility first decreases and then increases. The ruggedness of the terrain upon which a village is built has no significant effect. The ruggedness of the zone between a village and its nearest school exerts significant effects. These findings demonstrate that the two policies have created a pattern of spatial injustice that disadvantages peripheral villages, illustrating the need to attend to topography in efforts to provide equitable school access in rural mountainous areas.
Outdoor Space Elements in Urban Residential Areas in Shenzhen, China: Optimization Based on Health-Promoting Behaviours of Older People
Authors: Zhang Ling , Shao Kebin , Tang Wenfeng , Lau Stephen Siu Yu , Lai Hongzhan , Tao Yiqi
Published: May 2023

Given the ageing global population, it is important to promote “healthy ageing”. One of the most effective ways to achieve this is by prolonging the health of older people. Both the physical and mental well-being of older people are closely related to their living environment. Providing daily outdoor activities and enhancing the quality of public spaces and amenities in residential areas can encourage the adoption of healthy behaviours among older people. This study selected eight typical residential areas in Shenzhen, China, and analysed 40 outdoor public spaces. Video content obtained from fixed-point behavioural observation was entered into the Mangold INTERACT behavioural analysis system to extract the health behaviour data of older people. Regression analysis was then performed on the health behaviour data and the index data of the sample space elements. The results showed that several factors affect the outdoor health behaviours of older people. These factors include the scale of the outdoor space, the size of the hard ground area, the quality of the grey space, the green-looking rate, the accessibility of the site, the number of fitness facilities, and the richness of site functions. This study focused on a host of health-related behaviours such as rest, leisure, communication, and exercise. It confirmed the corresponding spatial needs of the elderly when engaging in the aforesaid activities. In this way, the quantitative research has supplemented previous studies by studying and evaluating the behaviour and activities of the elderly in specific settings. Through the analyses, a configuration model of outdoor space in residential areas was constructed with the aim of health promotion. Based on this model, a flexible and multilevel configuration list revealing seven specific types under three priorities is being proposed. The findings provide a scientific and effective strategy for optimising the quality of outdoor environments in residential areas. More specifically, the deployment of the Mangold INTERACT system to extract and quantify behavioural data enabled this study to overcome the limitations of traditional approaches to behavioural observation and recording. This provides a prelude for other quantitative research on the environment and behaviour.
The Expression of Time in Amahuaca Switch-Reference Clauses
Authors: Clem Emily
Published: May 2023

Many languages of lowland South America mark remoteness distinctions in their TAM systems. In Amahuaca (Panoan; Peru) multiple remoteness distinctions are made in the past and the future. I argue that the temporal remoteness morphemes (TRMs) of Amahuaca can be understood as indications of the remoteness of the event time relative to the utterance time in matrix environments. In dependent clauses, however, the picture is more complicated. By exploring adjunct switch-reference clauses, I show that TRMs in dependent clauses display a previously unreported ambiguity reminiscent of ambiguities found with adjunct tense. Specifically, they can relate the time of the adjunct clause event to the time of the matrix event or to the utterance time. I suggest that this ambiguity may arise from the availability of multiple interpretation sites for adjunct TRMs, with the possible interpretations being constrained by the temporal semantics of switch-reference markers themselves. This work thus contributes to the empirical understanding of how TRMs are interpreted in dependent clauses, suggesting interesting potential parallels to the interpretation of adjunct tense.
The Nature, Role, and Effects of Structured Input Activities
Authors: Benati Alessandro
Published: May 2023

This paper is the introductory paper of the Special Issue titled: “New Research on the Role and Effects of Structured Input in Assessing the Nature of Language Processing”. It provides a clear analysis of the nature and role of structured input activities in second language research and language pedagogy. It presents the main findings of genuine empirical research investigating the effectiveness of structured input on different forms and structures across different languages and among different populations. The paper provides suggestions for future research within this framework.
Impact of Microgravity and Other Spaceflight Factors on Retina of Vertebrates and Humans In Vivo and In Vitro
Authors: Grigoryan Eleonora N.
Published: May 2023

Spaceflight (SF) increases the risk of developmental, regenerative, and physiological disorders in animals and humans. Astronauts, besides bone loss, muscle atrophy, and cardiovascular and immune system alterations, undergo ocular disorders affecting posterior eye tissues, including the retina. Few studies revealed abnormalities in the development and changes in the regeneration of eye tissues in lower vertebrates after SF and simulated microgravity. Under microgravity conditions, mammals show disturbances in the retinal vascular system and increased risk of oxidative stress that can lead to cell death in the retina. Animal studies provided evidence of gene expression changes associated with cellular stress, inflammation, and aberrant signaling pathways. Experiments using retinal cells in microgravity-modeling systems in vitro additionally indicated micro-g-induced changes at the molecular level. Here, we provide an overview of the literature and the authors’ own data to assess the predictive value of structural and functional alterations for developing countermeasures and mitigating the SF effects on the human retina. Further emphasis is given to the importance of animal studies on the retina and other eye tissues in vivo and retinal cells in vitro aboard spacecraft for understanding alterations in the vertebrate visual system in response to stress caused by gravity variations.
Efficacy of Convalescent Plasma to Treat Long-Standing COVID-19 in Patients with B-Cell Depletion
Authors: Tomisti Luca , Angelotti Francesca , Lenzi Mirco , Amadori Francesco , Sarteschi Giovanni , Porcu Anna , Capria Anna-Lisa , Bertacca Gloria , Lombardi Stefania , Bianchini Guido , Vincenti Antonella , Cesta Novella
Published: May 2023

The use of antivirals, corticosteroids, and IL-6 inhibitors has been recommended by the WHO to treat COVID-19. CP has also been considered for severe and critical cases. Clinical trials on CP have shown contradictory results, but an increasing number of patients, including immunocompromised ones, have shown benefits from this treatment. We reported two clinical cases of patients with prolonged COVID-19 infection and B-cell depletion who showed rapid clinical and virological recovery after the administration of CP. The first patient in this study was a 73-year-old female with a history of follicular non-Hodgkin lymphoma previously treated with bendamustine followed by maintenance therapy with rituximab. The second patient was a 68-year-old male with chronic obstructive pulmonary disease, bipolar disorder, alcoholic liver disease, and a history of mantellar non-Hodgkin lymphoma treated with rituximab and radiotherapy. After the administration of CP, both patients showed a resolution of symptoms, improvement of their clinical conditions, and a negative result of the nasopharyngeal swab test. The administration of CP might be effective in resolving symptoms and improving clinical and virological outcomes in patients with B-cell depletion and prolonged SARS-CoV2 infections.
Interplay between Eimeria acervulina and Cryptosporidium parvum during In Vitro Infection of a Chicken Macrophage Cell Line (HD11)
Authors: Taha Shahinaz , Nguyen-Ho-Bao Tran , Berberich Lisa Maxi , Gawlowska Sandra , Daugschies Arwid , Rentería-Solís Zaida
Published: May 2023

Background: Eimeria acervulina is a frequent intestinal pathogen of chickens, causing economic impact on the poultry industry. Cryptosporidium parvum is a neglected parasite in chickens. However, because of its zoonotic potential, poultry cryptosporidiosis may pose a risk to public health. Little is known about the parasite–host interactions during coinfection with both parasites. In this study, we investigated the possible interactions during in vitro coinfection of E. acervulina and C. parvum in a chicken macrophage cell line (HD11). Methods: HD11 cells were inoculated with E. acervulina and C. parvum sporozoites and incubated 2, 6, 12, 24, and 48 h post infection (hpi). Mono-infections for each parasite were also investigated. Real-time PCR was used to quantify parasite replication. Additionally, macrophage mRNA expression levels of IFN-γ, TNF-α, iNOS, and IL-10 were measured. Results: For both parasites, multiplication was, in most groups, lower in the coinfection group (COIG) compared with mono-infections. However, at 6 hpi, the number of C. parvum copies was higher in co-infections. Intracellular replication started to decrease from 12 hpi onward, and it was almost undetectable by 48 hpi in all groups. Infections resulted in low expression of all cytokines, except at 48 hpi. Conclusions: Infection of avian macrophages with both E. acervulina and C. parvum seemed to hinder intracellular replication for both parasites in comparison to mono-infection. A clear reduction in intracellular parasites from 12 hpi onward details the important role potentially played by macrophages in host control of these parasites.
The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study
Authors: Petrovic Milos , Simic Tatjana , Djukic Tatjana , Radic Tanja , Savic-Radojevic Ana , Zekovic Milica , Durutovic Otas , Janicic Aleksandar , Milojevic Bogomir , Kajmakovic Boris , Zivkovic Marko , Bojanic Nebojsa , Bumbasirevic Uros , Coric Vesna
Published: May 2023

Members of the omega class of glutathione transferases (GSTs), GSTO1, and GSTO2, catalyze a range of reduction reactions as a part of the antioxidant defense system. Polymorphisms of genes encoding antioxidant proteins and the resultant altered redox profile have already been associated with the increased risk for testicular germ cell cancer (GCT) development. The aim of this pilot study was to assess the individual, combined, haplotype, and cumulative effect of GSTO1rs4925, GSTO2rs156697, and GSTO2rs2297235 polymorphisms with the risk for testicular GCT development, in 88 patients and 96 matched controls, through logistic regression models. We found that carriers of the GSTO1*C/A*C/C genotype exhibited an increased risk for testicular GCT development. Significant association with increased risk of testicular GCT was observed in carriers of GSTO2rs2297235*A/G*G/G genotype, and in carriers of combined GSTO2rs156697*A/G*G/G and GSTO2rs2297235*A/G*G/G genotypes. Haplotype H7 (GSTO1rs4925*C/GSTO2rs2297235*G/GSTO2rs156697*G) exhibited higher risk of testicular GCT, however, without significant association (p > 0.05). Finally, 51% of testicular GCT patients were the carriers of all three risk-associated genotypes, with 2.5-fold increased cumulative risk. In conclusion, the results of this pilot study suggest that GSTO polymorphisms might affect the protective antioxidant activity of GSTO isoenzymes, therefore predisposing susceptible individuals toward higher risk for testicular GCT development.
A Rule-Based Predictive Model for Estimating Human Impact Data in Natural Onset Disasters—The Case of a PRED Model
Authors: Rye Sara , Aktas Emel
Published: May 2023

Background: This paper proposes a framework to cope with the lack of data at the time of a disaster by employing predictive models. The framework can be used for disaster human impact assessment based on the socio-economic characteristics of the affected countries. Methods: A panel data of 4252 natural onset disasters between 1980 to 2020 is processed through concept drift phenomenon and rule-based classifiers, namely the Moving Average (MA). Results: Predictive model for Estimating Data (PRED) is developed as a decision-making platform based on the Disaster Severity Analysis (DSA) Technique. Conclusions: comparison with the real data shows that the platform can predict the human impact of a disaster (fatality, injured, homeless) with up to 3% error; thus, it is able to inform the selection of disaster relief partners for various disaster scenarios.
Tool Wear and Surface Integrity of γ-TiAl Cryogenic Coolant Machining at Various Cutting Speed Levels
Authors: Wang Xiangyu , Zhang Xiaoxia , Pan Duo , Niu Jintao , Fu Xiuli , Qiao Yang
Published: May 2023

High-speed machining of γ-TiAl alloy is a significant challenge due to high cutting temperatures. From the perspective of environmental protection and improving tool life, appropriate cooling strategies should be adopted. Compared with dry and conventional flood cooling conditions, the feasibility of machining γ-TiAl in cryogenic LN2 cooling conditions was discussed. The cutting force, tool wear and its mechanism, and surface roughness, as well as sub-surface morphology characteristics, were studied by combining macro and micro techniques. The results revealed that the wear morphology of the rake and flank face under the three cooling media shows different degrees. The crater wear of the rake face is expanded at high speeds and then progresses into more serve flaking and notching wear. The main wear pattern on the flank face is gradually transformed from adhesive wear to diffusion and oxidation wear at high speeds in dry machining. In the LN2 condition, the diffusion of workpiece elements and cutting-edge oxidation were restrained. The wear pattern is still mainly adhesive wear. In addition, cryogenic machining shows significant advantages in reducing cutting force, suppressing heat-affected zone, improving surface quality, and inhibiting micro-lamellar deformation.
Isoetin 2′-O-α-l-arabinopyranoside-5′-O-β-d-glucopyranoside
Authors: Galarza Pérez Mayra , Fell Fabian , Zheleva-Dimitrova Dimitrina , Zidorn Christian
Published: May 2023

Isoetin derivatives are a rare class of flavonoids with a rather erratic occurrence across the plant kingdom. The Cichorieae tribe of the Asteraceae family has proven to be a rich source and a centre of chemical diversity of this class of compounds. Here, we describe the chromatographic isolation and mainly NMR-based structure elucidation of a previously undescribed isoetin derivative from Leontodon hispidus L. (Asteraceae, Cichorieae). The chemophenetic relevance is discussed briefly.
The Efficient Way to Design Cooling Sections for Heat Treatment of Long Steel Products
Authors: Kotrbacek Petr , Chabicovsky Martin , Resl Ondrej , Kominek Jan , Luks Tomas
Published: May 2023

To achieve the required mechanical properties in the heat treatment of steel, it is necessary to have an adequate cooling rate and to achieve the desired final temperature of the product. This should be achieved with one cooling unit for different product sizes. In order to provide the high variability of the cooling system, different types of nozzles are used in modern cooling systems. Designers often use simplified, inaccurate correlations to predict the heat transfer coefficient, resulting in the oversizing of the designed cooling system or failure to provide the required cooling regime. This typically results in longer commissioning times and higher manufacturing costs of the new cooling system. Accurate information about the required cooling regime and the heat transfer coefficient of the designed cooling is critical. This paper presents a design approach based on laboratory measurements. Firstly, the way to find or validate the required cooling regime is presented. The paper then focuses on nozzle selection and presents laboratory measurements that provide accurate heat transfer coefficients as a function of position and surface temperature for different cooling configurations. Numerical simulations using the measured heat transfer coefficients allow the optimum design to be found for different product sizes.
Influence of Halides on Elastic and Vibrational Properties of Mixed-Halide Perovskite Systems Studied by Brillouin and Raman Scattering
Authors: Naqvi Furqanul Hassan , Junaid Syed Bilal , Ko Jae-Hyeon
Published: May 2023

The relationship between halogen content and the elastic/vibrational properties of MAPbBr3−xClx mixed crystals (x = 1.5, 2, 2.5, and 3) with MA = CH3NH3+ has been studied using Brillouin and Raman spectroscopy at room temperature. The longitudinal and transverse sound velocities, the absorption coefficients and the two elastic constants C11 and C44 could be obtained and compared for the four mixed-halide perovskites. In particular, the elastic constants of the mixed crystals have been determined for the first time. A quasi-linear increase in the sound velocity and the elastic constant C11 with increasing chlorine content was observed for the longitudinal acoustic waves. C44 was insensitive to the Cl content and very low, indicating a low elasticity to shear stress in mixed perovskites regardless of the Cl content. The acoustic absorption of the LA mode increased with increasing heterogeneity in the mixed system, especially for the intermediate composition where the Br and Cl ratio was 1:1. In addition, a significant decrease in the Raman-mode frequency of the low-frequency lattice modes and the rotational and torsional modes of the MA cations was observed with decreasing Cl content. It clearly showed that the changes in the elastic properties as the halide composition changes were correlated with the lattice vibrations. The present findings may facilitate a deeper understanding of the complex interplay between halogen substitution, vibrational spectra and elastic properties, and may also pave the way for optimizing the operation of perovskite-based photovoltaic and optoelectronic devices by tailoring their chemical composition.
A Rational Design of a CoS2-CoSe2 Heterostructure for the Catalytic Conversion of Polysulfides in Lithium-Sulfur Batteries
Authors: Zhang Bin , Ma Jiping , Cui Manman , Zhao Yang , Wei Shizhong
Published: May 2023

Lithium-sulfur batteries are anticipated to be the next generation of energy storage devices because of their high theoretical specific capacity. However, the polysulfide shuttle effect of lithium-sulfur batteries restricts their commercial application. The fundamental reason for this is the sluggish reaction kinetics between polysulfide and lithium sulfide, which causes soluble polysulfide to dissolve into the electrolyte, leading to a shuttle effect and a difficult conversion reaction. Catalytic conversion is considered to be a promising strategy to alleviate the shuttle effect. In this paper, a CoS2-CoSe2 heterostructure with high conductivity and catalytic performance was prepared by in situ sulfurization of CoSe2 nanoribbon. By optimizing the coordination environment and electronic structure of Co, a highly efficient CoS2-CoSe2 catalyst was obtained, to promote the conversion of lithium polysulfides to lithium sulfide. By using the modified separator with CoS2-CoSe2 and graphene, the battery exhibited excellent rate and cycle performance. The capacity remained at 721 mAh g−1 after 350 cycles, at a current density of 0.5 C. This work provides an effective strategy to enhance the catalytic performance of two-dimensional transition-metal selenides by heterostructure engineering.
Removal Performance of KOH-Modified Biochar from Tropical Biomass on Tetracycline and Cr(VI)
Authors: Wang Qingxiang , Yue Yan , Liu Wenfei , Liu Qing , Song Yu , Ge Chengjun , Ma Hongfang
Published: May 2023

Biochar can be used to address the excessive use of tetracycline and micronutrient chromium (Cr) in wastewater that potentially threatens human health. However, there is little information about how the biochar, made from different tropical biomass, facilitates tetracycline and hexavalent chromium (Cr(VI)) removal from aqueous solution. In this study, biochar was prepared from cassava stalk, rubber wood and sugarcane bagasse, then further modified with KOH to remove tetracycline and Cr(VI). Results showed that pore characteristics and redox capacity of biochar were improved after modification. KOH-modified rubber wood biochar had the highest removal of tetracycline and Cr(VI), 1.85 times and 6 times higher than unmodified biochar. Tetracycline and Cr(VI) can be removed by electrostatic adsorption, reduction reaction, π–π stacking interaction, hydrogen bonding, pore filling effect and surface complexation. These observations will improve the understanding of the simultaneous removal of tetracycline and anionic heavy metals from wastewater.
Strength Investigation and Prediction of Superfine Tailings Cemented Paste Backfill Based on Experiments and Intelligent Methods
Authors: Hu Yafei , Li Keqing , Zhang Bo , Han Bin
Published: May 2023

The utilization of solid waste for filling mining presents substantial economic and environmental advantages, making it the primary focus of current filling mining technology development. To enhance the mechanical properties of superfine tailings cemented paste backfill (SCPB), this study conducted response surface methodology experiments to investigate the impact of various factors on the strength of SCPB, including the composite cementitious material, consisting of cement and slag powder, and the tailings’ grain size. Additionally, various microanalysis techniques were used to investigate the microstructure of SCPB and the development mechanisms of its hydration products. Furthermore, machine learning was utilized to predict the strength of SCPB under multi-factor effects. The findings reveal that the combined effect of slag powder dosage and slurry mass fraction has the most significant influence on strength, while the coupling effect of slurry mass fraction and underflow productivity has the lowest impact on strength. Moreover, SCPB with 20% slag powder has the highest amount of hydration products and the most complete structure. When compared to other commonly used prediction models, the long-short term memory neural network (LSTM) constructed in this study had the highest prediction accuracy for SCPB strength under multi-factor conditions, with root mean square error (RMSE), correlation coefficient (R), and variance account for (VAF) reaching 0.1396, 0.9131, and 81.8747, respectively. By optimizing the LSTM using the sparrow search algorithm (SSA), the RMSE, R, and VAF improved by 88.6%, 9.4%, and 21.9%, respectively. The research results can provide guidance for the efficient filling of superfine tailings.
Evaluation of the Effect of Binary Fly Ash-Lime Mixture on the Bearing Capacity of Natural Soils: A Comparison with Two Conventional Stabilizers Lime and Portland Cement
Authors: Arias-Jaramillo Yhan P. , Gómez-Cano Diana , Carvajal Gloria I. , Hidalgo César A. , Muñoz Fredy
Published: May 2023

This study evaluates a binary mixture of fly ash and lime as a stabilizer for natural soils. A comparative analysis was performed on the effect on the bearing capacity of silty, sandy and clayey soils after the addition of lime and ordinary Portland cement as conventional stabilizers, and a non-conventional product of a binary mixture of fly ash and Ca(OH)2 called FLM. Laboratory tests were carried out to evaluate the effect of additions on the bearing capacity of stabilized soils by unconfined compressive strength (UCS). In addition, a mineralogical analysis to validate the presence of cementitious phases due to chemical reactions with FLM was performed. The highest UCS values were found in the soils that required the highest water demand for compaction. Thus, the silty soil added with FLM reached 10 MPa after 28 days of curing, which was in agreement with the analysis of the FLM pastes, where soil moistures higher than 20% showed the best mechanical characteristics. Furthermore, a 120 m long track was built with stabilized soil to evaluate its structural behavior for 10 months. An increase of 200% in the resilient modulus of the FLM-stabilized soils was identified, and a decrease of up to 50% in the roughness index of the FLM, lime (L) and Ordinary Portland Cement (OPC)-stabilized soils compared to the soil without addition, resulting in more functional surfaces.