bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of efficiency between two artificial insemination methods using fresh semen in domestic cat (Felis Catus)
Authors: Roshani Amin , Ekrami Behrang , Ghasemzadeh-Nava Hamid , Akbarinejad Vahid , Mohammadifard Homa , Zamanpoor Amin
Published: April 2023

The objective of this study was to compare pregnancy rates in domestic cats using fresh semen for the bilateral intrauterine insemination (BIUI) method and the novel uterine body insemination (UBI) method. Queens received a single injection of equine chorionic gonadotropin (eCG) (200 IU; IM) to induce ovarian follicular development and, after 83 h, an injection of human chorionic gonadotropin (hCG) (100 IU; IM) for final oocyte maturation and ovulation induction. Thirty-four hours after hCG administration, 3 × 106 fresh spermatozoa were used for insemination by the BIUI (n = 8 queens) or by the UBI (n = 7 queens) techniques respectively. Pregnancy rates were 75.00% (6/8) by BIUI and 42.85% (3/7) by the UBI method. The mean litter size was 3.0 ± 0.86 for the BIUI, and 2.0 ± 1.0 for the UBI method. Spontaneous abortion occurred on day 35 of pregnancy in one queen following the UBI method. Our findings showed that the BIUI of queens with fresh semen resulted in higher pregnancy rates than the novel UBI method; also, acceptable pregnancy rates were achieved following BIUI with fresh semen in the domestic cat.
Utilização de métodos alternativos como indutor de muda forçada em codornas poedeiras
Authors: Oliveira Ana Beatriz Fonseca de , Santos Graciene Conceição dos , Castro Charlene Karollaine Batista de , Batista Mailson Vasconcelos , Oliveira Diego , Oliveira Suellen Pena de
Published: April 2023

Objetivou-se avaliar métodos alternativos de restrição alimentar qualitativa e quantitativa em comparação à técnica convencional de muda forçada em codornas japonesas para o segundo ciclo de produção. O período de muda forçada teve duração de 10 dias e dois períodos de 21 dias de pós-muda. Foram utilizadas 72 codornas japonesas fêmeas, em final de produção com 72 semanas de idade. As aves foram distribuídas em delineamento inteiramente casualizado com três tratamentos e quatro repetições cada, com seis codornas por repetição. Os tratamentos experimentais foram: T1 – aves submetidas a três dias de jejum com posterior fornecimento de ração de postura à vontade por sete dias; T2 – aves que receberam alimentação controlada (10 g/ave/dia) por 10 dias; T3 – aves que receberam farelo de arroz à vontade por 10 dias. Durante todo o experimento todas as aves foram submetidas a iluminação natural com média de 12 horas de luz diária e água a vontade e após os 10 dias de muda foi fornecido ração de postura a vontade. A utilização do T2 (10 g/ave/dia de ração de postura) e de farelo de arroz como alimento alternativo no programa de muda forçada foi viável. Mesmo a perda de peso sendo abaixo do recomentado, as aves que receberam 10 g de ração ave/dia e farelo de arroz à vontade apresentaram resultados de desempenho e qualidade do ovo pós muda semelhantes às aves que ficaram em jejum, além de possibilitar melhores condições de bem-estar animal.
Micropipette tip intubation in rats as a replacement for conventional endotracheal tube intubation
Authors: Kim Myung-Good , Ryu eong-Ho , Lee Dong Min , Park Tae-Seo , Choi Ji-An
Published: April 2023

Endotracheal intubation is often necessary in the course of animal experiments, especially in craniofacial surgery. However, endotracheal intubation can be a major burden in this context. The authors performed simple and cost-saving method using a 200 μL yellow micropipette tip, and the success of this method was demonstrated by X-ray and autopsy. We used a total of 30 rats. After the rats were fixed with a plaster, the trachea and vocal cords were visualized with the tongue pulled back. Under direct visualization of the vocal cords, a curving micropipette tip was advanced into the trachea. This method can be learned quickly and applied successfully by general experimenters. We successfully intubated all 30 rats without any complications. The success rate of micropipette tip intubation was 100%. This procedure was performed by one experimenter within 2 to 3 minutes after induction of anesthesia. We demonstrated its superiority by X-ray and autopsy. Herein, we describe endotracheal intubation of rats using micropipette tips. To the best of our knowledge, this method is novel and represents the simplest and most efficient means of intubation in rats, providing an alternative to conventional endotracheal intubation.
Malignant melanoma associated with a plaque-type blue nevus of the cheek: a case report
Authors: Chung Yoon Kyu , Kim Min-Seok , Shin Jin Yong , Lee Nae-Ho , An Ae Ri , Roh Si-Gyun
Published: April 2023

Blue nevi, which are characterized by collections of pigment-producing melanocytes in the dermis, have a variety of clinicopathological characteristics. Plaque-type blue nevus (PTBN) is a variant of blue nevi. PTBN presents at birth or arises in early childhood, and it shows a combination of the features found in common blue nevus and cellular blue nevus. It is typically found on the dorsal surface of the hands and feet or on the head and neck, and it is usually benign and stable over time. However, reports have occasionally described malignant melanomas developing in or associated with a PTBN. Malignant blue nevi are most commonly found on the scalp. We report the case of an 88-year-old woman with a malignant melanoma associated with a PTBN of the cheek.
Spin-orbit torque efficiency improved by BiSePt alloy
Authors: He Hao-Bin , Lan Xiu-Kai , Ji Yang ,   State Key Laboratory for Superlattices and Microstructures, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China,   College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China
Published: April 2023

In order to achieve high-efficiency spin-orbit torque devices, higher charge-spin conversion efficiency, and lower resistivity are required in the strong spin-orbit coupling layer that provides the spin current. In this work we prepare BiSePt alloy/Co heterostructures with in-plane magnetic anisotropy by magnetron sputtering deposition. The alloy layer is deposited via one of two procedures, either co-sputtering or alternative-sputtering. We study the BiSePt alloy samples and find that the spin orbit torque (SOT) efficiency decreases with the increase of Pt component, which is attributed to the change of topological order of Bi2Se3 amorphous surface, caused by Pt doping. And the resistivity decreases with the increase of Pt component, which depends on the increase of metallic property. Due to the balance of these two competing mechanisms, the spin Hall conductivity of the alloy layer varies non-monotonically with the concentration ratio, and reach an optimal value at a ratio of 67% of Bi2Se3 component. With the increase of the Bi2Se3 component, the SOT efficiency, electrical resistivity and spin Hall conductance of the alloy layer show different trends. At about 20%–70%, they increase/decrease tardily. At about 70%–100%, the resistivity ascends more prominently than the SOT efficiency, which leads the spin Hall conductance to decrease. Comparing with using the co-sputtering deposition, the electrical conductivity and spin Hall angle of the alloy layer obtained using alternating sputtering deposition are small, which is attributed to the enhancing of interfacial scattering and the filter effect of Pt on the spin flow. In contrast to traditional pure heavy metal materials (such as Pt, Ta) and topological insulator materials like Bi2Se3, our BiSePt alloy devices obtained by co-sputtering deposition achieve industry-matched preparation conditions, greater SOT efficiency, and considerable electrical conductivity of the alloy layer, thus making further applications of SOT devices possible.
Predictive nonlinear modeling of malignant myelopoiesis and tyrosine kinase inhibitor therapy
Authors: Rodriguez Jonathan , Iniguez Abdon , Jena Nilamani , Tata Prasanthi , Liu Zhong-Ying , Lander Arthur D , Lowengrub John , Van Etten Richard A
Published: April 2023

Chronic myeloid leukemia (CML) is a blood cancer characterized by dysregulated production of maturing myeloid cells driven by the product of the Philadelphia chromosome, the BCR-ABL1 tyrosine kinase. Tyrosine kinase inhibitors (TKIs) have proved effective in treating CML, but there is still a cohort of patients who do not respond to TKI therapy even in the absence of mutations in the BCR-ABL1 kinase domain that mediate drug resistance. To discover novel strategies to improve TKI therapy in CML, we developed a nonlinear mathematical model of CML hematopoiesis that incorporates feedback control and lineage branching. Cell–cell interactions were constrained using an automated model selection method together with previous observations and new in vivo data from a chimeric BCR-ABL1 transgenic mouse model of CML. The resulting quantitative model captures the dynamics of normal and CML cells at various stages of the disease and exhibits variable responses to TKI treatment, consistent with those of CML patients. The model predicts that an increase in the proportion of CML stem cells in the bone marrow would decrease the tendency of the disease to respond to TKI therapy, in concordance with clinical data and confirmed experimentally in mice. The model further suggests that, under our assumed similarities between normal and leukemic cells, a key predictor of refractory response to TKI treatment is an increased maximum probability of self-renewal of normal hematopoietic stem cells. We use these insights to develop a clinical prognostic criterion to predict the efficacy of TKI treatment and design strategies to improve treatment response. The model predicts that stimulating the differentiation of leukemic stem cells while applying TKI therapy can significantly improve treatment outcomes.
Making it hard to replicate
Authors: Ng Billy Wai-Lung , Scheeff Stephan , Xeque Amada Josefina
Published: April 2023

Understanding how to harden liquid condensates produced by influenza A virus could accelerate the development of novel antiviral drugs.
Side-effects of hyperthermic intraperitoneal chemotherapy in patients with gastrointestinal cancers
Authors: Hu Jiyun , Wang Zhenxing , Wang Xinrun , Xie Shucai
Published: April 2023

Background

Hyperthermic intraperitoneal chemotherapy (HIPEC) produces unwanted side-effects that are mainly caused by chemotherapeutic drugs in the treatment of gastrointestinal (GI) cancers, and these effects have not been systematically summarized. The aim of this article was to provide a comprehensive overview of the side-effects of HIPEC for GI cancers and propose practical strategies for adverse event management.

Methodology

PubMed, Web of Science, and the Cochrane Library were systematically searched for side-effects of HIPEC in GI cancers prior to October 20, 2022. A total of 79 articles were included in this review.

Results

Adverse events, such as enterocutaneous digestive fistulas, GI tract perforation, neutropenia, postoperative bleeding, ventricular tachycardia, hyperglycemia, hypocalcemia, renal impairment, encapsulating peritoneal sclerosis, scrotal ulceration, and sarcopenia were described, and their clinical management was discussed. These side-effects involve the digestive, hematopoietic, circulatory, metabolic, and urinary systems. Effective methods for adverse event management included an expert multidisciplinary team, replacing chemotherapy drugs, using Chinese medicine, and careful preoperative assessments.

Conclusion

The side-effects of HIPEC are frequent and can be minimized by several effective methods. This study proposes practical strategies for adverse event management of HIPEC to assist physicians in choosing the optimal treatment method.
Lenguaje evaluativo en las narraciones personales de niños bilingües español-inglés
Authors:   Universidad Central de Venezuela, Florida Atlantic University, Shiro Martha
Published: April 2023

El discurso narrativo refleja la cultura del hablante y requiere del dominio de habilidades socioculturales, cognitivas y emocionales, además de destrezas lingüísticas y pragmáticas. El narrador, mediante el uso del lenguaje evaluativo, construye un eje temporal de eventos y un plano expresivo que transmite sus actitudes. Este trabajo busca determinar cómo influye el bilingüismo en la producción narrativa de niños en edad preescolar, especialmente en el plano expresivo. Analizo las narraciones de experiencia personal de 18 niños bilingües y 15 monolingües de cinco años. Los resultados sugieren, por una parte, que no hay diferencias en las mediciones globales de vocabulario y lenguaje evaluativo entre los niños bilingües y sus pares monolingües. Por otra parte, se encontraron variaciones en el uso de ciertas expresiones evaluativas, lo que puede relacionarse con las diferencias culturales que provienen de las prácticas discursivas en sus comunidades. Los niños monolingües se refieren más a los procesos mentales, mientras que los bilingües usan más elementos descriptivos. Este estudio permite conocer mejor el desarrollo del discurso narrativo oral en niños bilingües y puede ayudar a los docentes a facilitar el desarrollo de las habilidades de lectoescritura.
O Endomarketing como ferramenta para melhoria da eficiência nas operações de serviços: um estudo de caso na empresa Alfa e Ômega
Authors: Da Silva Lizandra Oliveira , Assunção David Mayck Vale , Rocha Fabiano da Silva , Roberto José Carlos Alves , Pinto Junior José Roberto Lira
Published: April 2023

O tema desse artigo tem como finalidade apresentar aos profissionais de gestão, o endomarketing como uma ferramenta de melhoria nas operações em pequenas e médias empresas, onde temos a eficiência a ser alcançada por meio de sua implementação. Mediante a isto estabeleceu-se o seguinte questionamento: Como a introdução de estratégias do endomarketing podem contribuir com a melhoria da eficiência nas operações de serviços? Este artigo apresenta a importância da eficiência do endomarketing para os coloboradores e organizações, onde  buscamos a motivação para melhorar as operações de serviços . O artigo apresentado tem como objetivo geral: Recomendar o endomarketing como ferramenta para a melhoria da eficiência nas operações de serviços de pequenas e médias empresas. Em vista disso, este artigo é composto por pesquisa qualitativa, através de livros e artigos, onde foram coletadas vários conceitos da área. Como: explanar o endomarketing como uma ferramenta estratégica para melhoria da eficiência nas operações; abordar as vantagens da implementação do endomarketing nas organizações e apresentar ações que auxiliam o endomarketing na gestão estratégica das operações nas empresas para que consigam melhores resultados. Com esse artigo acredita-se mostrar o endomarketing como ferramenta estratégica a ser implementada para melhorar grandemente a eficiência nas operações dentro das organizações.
Neurological Complications after Deceased Donor Liver Transplant: A Case Series from a Public Sector Hospital
Authors: Rathinasamy Kanimozhi , Logidasan Satish , Prakasam Karthik Arun , Karthik SR , Anjeneyan Gowri Shankar
Published: April 2023

This is a case series of neurological complications which occurred after Deceased Donor Liver Transplant (DDLT) in a public sector hospital. The clinical presentations were bizarre. This is because patients with End Stage Liver Disease (ESLD) commonly have hepatic encephalopathy and postoperatively can present with similar clinical picture, like tremors and altered mentation. The first patient in the series presented with tremors due to Calcineurin Inhibitor (CNI) toxicity, second had Posterior Reversible Encephalopathy Syndrome (PRES), third with Osmotic Demyelination Syndrome (ODS) with coma and fourth with Extrapyramidal Syndrome (EPS). Patients 1 and 4 had involuntary movements and other clinical manifestations which interfered with recuperation in the postoperative period. So, the dosages of the immunosuppressants were adjusted and serum tacrolimus assay were serially monitored on alternate days. Case 3 developed PRES due to hypertension, as a result of high serum tacrolimus levels. The patient presented with headache and seizures, which if uncontrolled would have been lethal. In Case 4 clinical features and management of a patient who suffered prolonged coma due to ODS for about three months has been discussed. Also, the same patient had a cerebral infarct due to embolic phenomenon inspite of thromboprophylaxis. From this case series, it needs to be emphasised that postoperative occurrence of neurological complications are likely. So careful selection of the recipients, steady titration of immunosuppressants and watchful monitoring of the neurological signs are essential to improve the outcome of the transplant. Imaging of the brain, preferably Magnetic Resonance Imaging (MRI) should not be delayed to rule out other differential diagnosis.
The Snowstorm
Authors: Van Hala Sonja
Published: April 2023

In this narrative medicine essay, a family physician takes a moment-by-moment, one-step-at-a-time approach to move from her couch to recovery from treatment for lymphoblastic leukemia to finally reaching the cross-country ski trails.
Hierarchical iterative identification algorithms for a nonlinear system with dead‐zone and saturation nonlinearity based on the auxiliary model
Authors: Sun Shunyuan , Wang Xiao , Ding Feng
Published: April 2023

This paper investigates the identification problem of an output‐error nonlinear system with saturation and dead‐zone nonlinearity. Introducing a switching function and by means of the auxiliary model identification idea, an auxiliary model hierarchical least squares‐based iterative algorithm is proposed for estimating the parameters of the nonlinear system. Based on the hierarchical identification model, an auxiliary model hierarchical gradient‐based iterative algorithm is presented for the nonlinear system by utilizing the gradient search. In order to take full advantage of the system data, an auxiliary model hierarchical multi‐innovation gradient‐based iterative algorithm is derived for the nonlinear system according to the multi‐innovation identification theory. Finally, the numerical simulation results illustrate the effectiveness of the proposed algorithms.
Synergistic Oxidative Damage with Gene Therapy Potentiates Targeted Sterilization of Broad‐Spectrum Microorganisms
Authors: Huang Shan , Song Yuexin , Chen Xiaojun , Min Qianhao , Zhang Jian‐Rong , Zhu Jun‐Jie
Published: April 2023

Nanoparticle‐based approaches addressed the barriers to antibiotic resistance faced by traditional antimicrobial agents. However, nanotherapies against multibacterial infections still suffered from the lack of broad‐spectrum targeting ability and the mono‐inhibition pathway. In this study, a multimodality therapeutic nanoplatform (denoted as Asza) is introduced, which combines specific recognition, synergistic oxidative damage, and gene therapy, to effectively inhibit the emergence of bacterial resistance, achieving broad‐spectrum sterilization activity against two Gram‐positive (B. subt, S. epider) and two Gram‐negative bacteria (E. coli, E. aero). In addition to the oxidative damage generated from gold nanoclusters, DNA aptamer, and CRISPR‐Cas modules are combined in the Asza to recognize multiple bacteria and cleave the ftsz gene with high specificity, allowing precision treatment of multibacterial infections without damaging surrounding healthy cells. Furthermore, multimodal antimicrobial strategies can reduce the risk of the generation of bacterial resistance to single‐modality therapy and significantly boost the efficiency of antibacterial therapy. This study offers a promising approach to advance the applications of nanomaterials in clinical antimicrobial therapy.
Highly Stretchable and Strain‐Insensitive Liquid Metal based Elastic Kirigami Electrodes (LM‐eKE)
Authors: Choi Hyesu , Luo Yichi , Olson Gina , Won Phillip , Shin Joo Hwan , Ok Jehyung , Yang Ye Ji , Kim Tae‐il , Majidi Carmel
Published: April 2023

Kirigami, a traditional paper‐cutting art, is a promising method for creating mechanically robust circuitry for unconventional devices capable of extreme stretchability through structural deformation. In this study, this design approach is expanded upon by introducing Liquid Metal based Elastic Kirigami Electrodes (LM‐eKE) in which kirigami‐patterned soft elastomers are coated with eutectic gallium‐indium (EGaIn) alloy. Overcoming the mechanical and electrical limitations of previous efforts with paper‐like kirigami, the all soft LM‐eKE can be stretched to 820% strain while the electrical resistance only increases by 33%. This is enabled by the fluidic properties of the EGaIn coating, which maintains high electrical conductivity even as the elastic substrate undergoes extreme deformation. Applying the LM‐eKE to human knee joints and fingers, the resistance change during physical activities is under 1.7%, thereby allowing for stable electrical operation of wearable health monitoring devices for tracking electroencephalogram (EEG) signals and other physiological activity.
Platelet Membrane Fragment Self‐Assembled Oral Hydrogel Microspheres for Restoring Intestinal Microvascular Injury
Authors: Liu Hua , Cai Zhengwei , Wang Fei , Ruan Huitong , Zhang Chen , Zhong Jie , Wang Zhengting , Cui Wenguo
Published: April 2023

Non‐invasive management of intestinal microvascular injury with hemorrhage under constant biochemical‐mechanical encroachment of luminal contents is a major clinical challenge. Herein, an oral hydrogel microsphere is designed, encapsulating the platelet membrane fragment self‐assembled nanohydrogel fabricated by double‐crosslinking of methacrylated hyaluronic acid and Rebamipide‐loaded dendrimer (Rng@PMS), for self‐localization of hemorrhagic focus and intensive management of intestinal microvascular injury via suppression of inflammation‐mediated tissue injury and reintegration of the damaged mucosal barrier. The results indicate that Rng@PMS effectively adheres to exposed collagen on injured microvasculature and attaches to over 90% of activated macrophages within 10 min, endowing Rng@PMS with a significantly prolonged enteral dwell time (over 24 hours). Importantly, Rng@PMS generated from alginate is designed for oral colon‐targeted delivery to avoid the erosion of gastrointestinal contaminants. In vivo study reveals oral administration of microspheres in murine hematochezia model upregulates the intestinal barrier proteins zonula occludens‐1, Occludin, and muc2, and inhibits infiltration of neutrophils, dendritic cells, and macrophages in hemorrhagic foci, thereby reducing the hematochezia score from 4 to 0.8, and the pathology score from 5.3 to 0.5. Oral microspheres for in situ management of intestinal microvascular injury may be applicable more broadly to noninvasively treat diseases with symptoms of mucosal hemorrhage.
Trap‐Dependent Optical/Thermal Stimulated Luminescence of Gallate Phosphors Charged by UV–visible–NIR light for Multiplexed Data Storage
Authors: Gao Dangli , Wang Zhigang , Pang Qing , Kuang Qingqing , Gao Feng , Zhang Xiangyu , Yun Sining , Wang Xiaojun
Published: April 2023

UV‐charged optical stimulated single‐band phosphors are being used for single‐level optical data storage because of their ability to easily write/read information by light. In contrast, UV–visible–NIR rechargeable optical stimulated double‐band phosphor is urgently necessary for multiplexed high‐throughput optical data storage. Here, the authors design and synthesize Mn2+,Cr3+ co‐doped zinc gallate storage persistent phosphor that exhibits strong red‐green bicolor persistent luminescence (PersL). Besides the traditional ultraviolet charging, the phosphor can be effectively and repeatedly charged by using visible and NIR light by one‐ or two‐photon process. Interestingly, red and green PersL exhibit the opposite temperature dependence, which is extremely important for multilevel information storage. The mechanism of temperature‐dependent PersL intensity based on different trap types and filling approaches for the double luminescence centers is proposed. The phosphor promises a sophisticated application in optical data storage of wavelength multiplexing.
Identification and Characterization of the Wilms Tumor Cancer Stem Cell
Authors: Petrosyan Astgik , Villani Valentina , Aguiari Paola , Thornton Matthew E. , Wang Yizhou , Rajewski Alex , Zhou Shengmei , Cravedi Paolo , Grubbs Brendan H. , De Filippo Roger E. , Sedrakyan Sargis , Lemley Kevin V. , Csete Marie , Da Sacco Stefano , Perin Laura
Published: April 2023

A nephrogenic progenitor cell (NP) with cancer stem cell characteristics driving Wilms tumor (WT) using spatial transcriptomics, bulk and single cell RNA sequencing, and complementary in vitro and transplantation experiments is identified and characterized. NP from WT samples with NP from the developing human kidney is compared. Cells expressing SIX2 and CITED1 fulfill cancer stem cell criteria by reliably recapitulating WT in transplantation studies. It is shown that self‐renewal versus differentiation in SIX2+CITED1+ cells is regulated by the interplay between integrins ITGβ1 and ITGβ4. The spatial transcriptomic analysis defines gene expression maps of SIX2+CITED1+ cells in WT samples and identifies the interactive gene networks involved in WT development. These studies define SIX2+CITED1+ cells as the nephrogenic‐like cancer stem cells of WT and points to the renal developmental transcriptome changes as a possible driver in regulating WT formation and progression.
Multiphysical Field Modulated VO2 Device for Information Encryption
Authors: Zhao Shanguang , Li Liang , Hu Changlong , Li Bowen , Liu Meiling , Zhu Jinglin , Zhou Ting , Shi Weidong , Zou Chongwen
Published: April 2023

In the information explosion society, information security is highly demanded in the practical application, which raised a surge of interest in designing secure and reliable information transmission channels based on the inherent properties of emerging devices. Here, an innovative strategy to achieve the data encryption and reading during the data confidential transmission based on VO2 device is proposed. Owing to the specific insulator‐to‐metal transition property of VO2, the phase transitions between the insulator and metallic states are modulated by the combination of electric field, temperature, and light radiation. These external stimulus‐induced phase diagram is directly associated with the defined VO2 device, which are applicable for control the “0” or “1” electrical logic state for the information encryption. A prototype device is fabricated on an epitaxial VO2 film, which displayed a unique data encryption function with excellent stability. The current study not only demonstrated a multiphysical field‐modulated VO2 device for information encryption, but also supplied some clues for functional devices applications in other correlated oxide materials.
Accurate and Rapid Detection of Peritoneal Metastasis from Gastric Cancer by AI‐Assisted Stimulated Raman Molecular Cytology
Authors: Chen Xun , Wu Zhouqiao , He Yexuan , Hao Zhe , Wang Qi , Zhou Keji , Zhou Wanhui , Wang Pu , Shan Fei , Li Zhongwu , Ji Jiafu , Fan Yubo , Li Ziyu , Yue Shuhua
Published: April 2023

Peritoneal metastasis (PM) is the mostcommon form of distant metastasis and one of the leading causes of death in gastriccancer (GC). For locally advanced GC, clinical guidelines recommend peritoneal lavage cytology for intraoperative PM detection. Unfortunately, current peritoneal lavage cytology is limited by low sensitivity (<60%). Here the authors established the stimulated Raman molecular cytology (SRMC), a chemical microscopy‐based intelligent cytology. The authors firstly imaged 53 951 exfoliated cells in ascites obtained from 80 GC patients (27 PM positive, 53 PM negative). Then, the authors revealed 12 single cell features of morphology and composition that are significantly different between PM positive and negative specimens, including cellular area, lipid protein ratio, etc. Importantly, the authors developed a single cell phenotyping algorithm to further transform the above raw features to feature matrix. Such matrix is crucial to identify the significant marker cell cluster, the divergence of which is finally used to differentiate the PM positive and negative. Compared with histopathology, the gold standard of PM detection, their SRMC method could reach 81.5% sensitivity, 84.9% specificity, and the AUC of 0.85, within 20 minutes for each patient. Together, their SRMC method shows great potential for accurate and rapid detection of PM from GC.
Higher systolic blood pressure in early‐mid adulthood is associated with poorer cognitive performance in those with a dominantly inherited Alzheimer's disease mutation but not in non‐carriers. Results from the DIAN study
Authors: Xu Ying , Aung Htein Linn , Bateman Randall J. , Brooks William S. , Chhatwal Jasmeer , Day Gregory S. , Fagan Anne M. , Farlow Martin R. , Gordon Brian , Kehoe Patrick G. , Levin Johannes , Mori Hiroshi , Morris John C. , Wharton Whitney , Humburg Peter , Schofield Peter R. , Peters Ruth ,   Dominantly Inherited Alzheimer Network (DIAN)
Published: April 2023

BACKGROUND

The Dominantly Inherited Alzheimer Network (DIAN) is a longitudinal observational study that collects data on cognition, blood pressure (BP), and other variables from autosomal‐dominant Alzheimer's disease mutation carriers (MCs) and non‐carrier (NC) family members in early to mid‐adulthood, providing a unique opportunity to evaluate BP and cognition relationships in these populations.

METHOD

We examined cross‐sectional and longitudinal relationships between systolic and diastolic BP and cognition in DIAN MC and NC.

RESULTS

Data were available from 528 participants, who had a mean age of 38 (SD = 11) and were 42% male and 61% MCs, at a median follow‐up of 2 years. Linear‐multilevel models found only cross‐sectional associations in the MC group between higher systolic BP and poorer performance on language (β = −0.181 [−0.318, −0.044]), episodic memory (−0.212 [−0.375, −0.049]), and a composite cognitive measure (−0.146 [−0.276, −0.015]). In NCs, the relationship was cross‐sectional only and present for language alone.

DISCUSSION

Higher systolic BP was cross‐sectionally but not longitudinally associated with poorer cognition, particularly in MCs. BP may influence cognition gradually, but further longitudinal research is needed.
Comparing machine learning‐derived MRI‐based and blood‐based neurodegeneration biomarkers in predicting syndromal conversion in early AD
Authors: Cai Yuan , Fan Xiang , Zhao Lei , Liu Wanting , Luo Yishan , Lau Alexander Yuk Lun , Au Lisa Wing Chi , Shi Lin , Lam Bonnie Y. K. , Ko Ho , Mok Vincent Chung Tong
Published: April 2023

Introduction

We compared the machine learning‐derived, MRI‐based Alzheimer's disease (AD) resemblance atrophy index (AD‐RAI) with plasma neurofilament light chain (NfL) level in predicting conversion of early AD among cognitively unimpaired (CU) and mild cognitive impairment (MCI) subjects.

Methods

We recruited participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI) who had the following data: clinical features (age, gender, education, Montreal Cognitive Assessment [MoCA]), structural MRI, plasma biomarkers (p‐tau181, NfL), cerebrospinal fluid biomarkers (CSF) (Aβ42, p‐tau181), and apolipoprotein E (APOE) ε4 genotype. We defined AD using CSF Aβ42 (A+) and p‐tau181 (T+). We defined conversion (C+) if a subject progressed to the next syndromal stage within 4 years.

Results

Of 589 participants, 96 (16.3%) were A+T+C+. AD‐RAI performed better than plasma NfL when added on top of clinical features, plasma p‐tau181, and APOE ε4 genotype (area under the curve [AUC] = 0.832 vs. AUC = 0.650 among CU, AUC = 0.853 vs. AUC = 0.805 among MCI) in predicting A+T+C+.

Discussion

AD‐RAI outperformed plasma NfL in predicting syndromal conversion of early AD.

Highlights

AD‐RAI outperformed plasma NfL in predicting syndromal conversion among early AD.

AD‐RAI showed better metrics than volumetric hippocampal measures in predicting syndromal conversion.

Combining clinical features, plasma p‐tau181 and apolipoprotein E (APOE) with AD‐RAI is the best model for predicting syndromal conversion.
LC–MS/MS bioanalytical method for the quantitative analysis of nifedipine, bisoprolol, and captopril in human plasma: Application to pharmacokinetic studies
Authors: Abdel‐Megied Ahmed M. , Kovalenko Sergiy , Elbarbry Fawzy A. , Piponski Marjan , Oleshchuk Oleksandra , El Deeb Sami , Magdy Galal , Belal Fathalla , Grochovuy Taras , Logoyda Liliya
Published: April 2023

In this study, the development and validation of an accurate and highly sensitive LC–MS/MS method were performed for the estimation of nifedipine, bisoprolol and captopril in real human plasma. Liquid–liquid extraction using tert‐butyl methyl ether was efficiently applied for extraction of the analytes from plasma samples. The chromatographic separation was carried out using an isocratic elution mode on the X‐terra MS C18 column (4.6 × 50 mm, 3.5 μm). The mobile phase consisted of methanol–0.1% formic acid (95:5, v/v) for determination of nifedipine and bisoprolol and acetonitrile–0.1% formic acid (70:30, v/v) for determination of captopril with a flow rate of 0.5 ml/min. Acceptable results regarding the different validation characteristics of the analytes were obtained in accordance with US Food and Drug Administration recommendations for bioanalytical methods. The developed approach was linear over concentration ranges of 0.5–130.0, 50.0–4,500.0 and 0.3–30.0 ng/ml for nifedipine, captopril and bisoprolol, respectively. The method revealed a sufficient lower limit of quantification in the range of 0.3–50.0 ng/ml, as well as high recovery percentages, indicating high bioanalytical applicability. The proposed method was efficiently applied to a pharmacokinetic evaluation of a fixed‐dose combination of the analytes in healthy male volunteers.
Acellular dermal matrix for the treatment of multiple gingival recession defects associated with carious and previously restored cervical lesions: A case report with 10 years of follow‐up
Authors: Boeriu Sorin , Steigmann Larissa , Di Gianfilippo Riccardo
Published: April 2023

Background

Limited evidence exists on the outcome of the modified coronally advanced tunnel (MCAT) with acellular dermal matrix (ADM) for the treatment of gingival recession defects (GRD) especially when complicated by restored cervical lesions. Therefore, the aim of this case report was to assess the short‐ and long‐term clinical outcomes of maxillary Type 1 recession defects (RT1) associated with restored cervical lesions treated with MCAT with ADM.

Methods and Results

A 43‐year‐old female patient, presented with multiple adjacent RT1 recessions in the left maxilla, previously treated with overhanging cervical resin restorations. The case was approached with a careful evaluation of the diagnostic determinants of root coverage, removal of the aberrant resin restorations, treatment with MCAT with ADM, and periodical evaluation over a follow‐up of 10 years.

The treatment was followed by complete root coverage, improvement of gingival phenotype, and perfect root coverage esthetic score. Outcomes were periodically assessed and were maintained over 10 years of follow‐up.

Conclusion

MCAT with ADM is an effective technique for the treatment of multiple GRD complicated by cervical restorations. Complete root coverage and excellent esthetic outcomes were obtained and maintained in the long term.
Paraneoplastic syndrome of arthropathies as presenting sign for non‐small cell lung cancer
Authors: Lee Alexander Kyle , Singh Gurpreet , Lewis Cleveland W.
Published: April 2023

The aim of this study is to showcase an example of paraneoplastic syndrome, so other healthcare providers can be aware of early NSCLC manifestations. This is also important so providers do not dismiss review of symptoms that may not seem pertinent.
A case of fatal disseminated adenovirus and drug‐resistant Pneumocystis pneumonia in a patient who received chemotherapy for mantle cell lymphoma
Authors: Yu Bo , Saravanan Lakshmi , Tran Dena H. , Davies Alexander J. , Kaur Harpreet , Naraynanan Shivakumar , Verceles Avelino C. , Kim Hyeong J.
Published: April 2023

Adenovirus (ADV) may cause severe complications in hematopoietic stem cell transplant recipients, but disseminated ADV infections in patients who received chemotherapy alone for hematological malignancies are poorly understood due to the rarity of cases. Concomitant infection with Pneumocystis (PCP) is extremely rare. Despite being diagnostically challenging, a more specific workup needs to be initiated with a low threshold in patients who are exposed to agents with the potential to suppress T cells. We report a fatal case of disseminated ADV and drug‐resistant PCP pneumonia in a patient with mantle cell lymphoma who had only received combination chemotherapy. A 75‐year‐old man who was diagnosed with mantle cell lymphoma 10 months prior was admitted for mild hypoxic respiratory failure. Bendamustine, Rituximab, Cytarabine regimen had resulted in complete remission of his lymphoma, with the last cycle of chemotherapy administered 3 months prior to admission. CT of the chest revealed ground‐glass opacities concerning pneumonia. Initial laboratory tests were remarkable for mild leukopenia. The respiratory viral panel was only positive for ADV. He did not respond to empiric antibiotics for community‐acquired pneumonia and Trimethoprim / Sulfamethoxazole given later for positive Beta D Glucan (BDG) suggestive of Pneumocystis pneumonia. Then, he developed hemorrhagic cystitis, followed by liver and renal function derangement that prompted checking serum ADV viral load by polymerase chain reaction (PCR). This test took 1 week to return, with a viral load of 50, 000 copies/mL suggesting disseminated ADV infection. Despite initiation of Cidofovir, multi‐organ failure continued to progress, and the follow‐up viral load had doubled on Day 2. The patient passed away the same day shortly after transition to comfort care. T cell suppression seems to be a risk factor for disseminated ADV disease. Clinicians may need to maintain a low threshold to send serum quantitative ADV PCR when symptoms are not improved by antimicrobial treatment for more conventional infections in patients who received agents that are known to suppress T cells, such as Bendamustine.
Cutaneous vasculitis after COVID‐19 vaccination in a 41‐year‐old male
Authors: Pournazari Mehran , Assar Shirin , Farsad Faraneh , Mohamadzadeh Dena
Published: April 2023

Leukocytoclastic vasculitis could be a possible adverse event of different SARS‐CoV‐2 vaccines. Clinicians and manufacturers should be aware of this adverse event for appropriate diagnosis and treatment.
A case of superior mesenteric artery syndrome characterized by deep forward bending posture in a cross‐legged position on the floor
Authors: Isono Hiroki , Nakanishi Koki
Published: April 2023

Key Clinical Message

A 17‐year‐old healthy male presents with severe epicardial pain and frequent vomiting 1 h after lunch, preferring to sit cross‐legged on a stretcher with a deep forward bending posture along with difficulty lying down. SMA syndrome should be considered in the differential diagnosis of patients demonstrating such posture.
Peroxymonosulfate‐Assisted Phenol Degradation via a Magnetic Covalent‐Triazine‐Framework‐Based Photocatalyst
Authors: Ai Lvye , Wang Qian , Cui Fuzhi , Jiang Guofang
Published: April 2023

The development of efficient heterostructures combining covalent organic frameworks (COFs) and ideal semiconductors can significantly improve photocatalytic performance for pollutant degradation. Herein, we present the design, synthesis, and characterization of a core‐shell‐structured nanocomposite comprising covalent triazine framework‐encased Fe3O4 magnetic particles employed as a heterojunction photocatalyst for activating peroxymonosulfate (PMS) in phenol degradation. The distinctive internal structure between the TpMa shell (Tp=2,4,6‐trihydroxy‐1,3,5‐benzenetricarboxaldehyde, Ma=melamine) and the Fe3O4 core (Fe3O4@TpMa) facilitated charge transfer and accelerated charge separation. Furthermore, PMS served as an electron acceptor, enhancing photogenerated charge separation and maximizing the production of reactive oxygen species. The Fe3O4@TpMa/PMS system demonstrated remarkable photocatalytic performance and stability, achieving complete phenol degradation (10 mg L−1) in 40 min. The exceptional photocatalytic activity resulted from the synergistic effect of ⋅OH, SO4⋅−, O2⋅−, 1O2, and h+ generated in the Fe3O4@TpMa/PMS system during the degradation process. Overall, this material offers excellent potential for solar‐driven pollutant degradation and enables the development of COF‐based materials for wastewater treatment applications.
Catalytic Promiscuity of Horseradish Peroxidase: Aerobic Oxidative Regeneration of Oxidized Nicotinamide Cofactors
Authors: Dai Jian‐Rong , Sha M. S. Jiang‐Tao , Zong Min‐Hua , Li Ning
Published: April 2023

NAD(P)+ regeneration is of great importance for dehydrogenase (DH)‐catalyzed oxidations. In this work, we report horseradish peroxidase (HRP)‐catalyzed aerobic oxidation of the reduced nicotinamide cofactors, including natural and non‐natural ones, into the oxidized form at neutral and alkaline pH enabled by external amino acids (AAs). HRP showed promiscuous yet extremely low oxidase activity toward NADH at pH 7 in the absence of both AAs and mediators, as evidenced by the observation of compound III. The mechanistic study revealed that AAs as decoy molecules (likely act as acid‐base catalysts) and mediators significantly promoted the formation of compound III as well as its decay back to the ground state. The applicability of the aerobic cofactor regeneration method was demonstrated by the HRP total turnover number up to 88 000 as well as high yields (91–99 %) in bi‐enzymatic synthesis of carboxylic acids. The present work offers an alternative method to recycle NAD(P)+. Also, it may open up possibilities for HRP‐catalyzed aerobic oxidations.
Influencing factors of kinesiophobia in older patients with chronic heart failure: A structural equation model
Authors: Qin Jingwen , Xiong Juanjuan , Chen Chen , Wang Xue , Gao Ya , Zhou Yan , Zheng Guixiang , Gong Kaizheng
Published: April 2023

Background

Our recent study has demonstrated that kinesiophobia is common in Chinese inpatients with chronic heart failure (CHF). Symptoms of heart failure (HF), coping mode, self‐efficacy for exercise (SEE), and social support have been reported to be associated with kinesiophobia. However, little is known about the relationships between these four variables and kinesiophobia in older patients with CHF.

Objective

To test a model of influencing factors of kinesiophobia in older CHF patients.

Methods

A cross‐sectional design was conducted from January 2021 to October 2021. The general information questionnaire, the Chinese version of the Tampa Scale for Kinesiophobia Heart (TSK‐SV Heart‐C), Symptom Status Questionnaire‐Heart Failure, SEE, the Medical Coping Modes Questionnaire, and Social Support Rating Scale were used. Spearman correlation analysis and structural equation model (SEM) were performed for data analysis.

Results

A total of 270 older patients with CHF were recruited. Symptom status of HF (r = 0.455, p < .01), avoidance coping mode (r = 0.393, p <.01), and yielding coping mode (r = 0.439, p < .01) were positively correlated with kinesiophobia, while SEE (r = −0.530, p < .01), facing coping mode (r = −0.479, p < .01), and social support (r = −0.464, p < .01) were negatively correlated with kinesiophobia. SEM analysis showed that social support could affect kinesiophobia through the mediating variables of symptom status of HF, avoidance coping mode, and exercise self‐efficacy.

Conclusions

Symptoms of HF, coping mode, SEE, and social support may play role in kinesiophobia in older CHF patients. We should pay more attention to the synergies among these four variables in the improvement of kinesiophobia.
Safety Profile of Eltrombopag in Different Age Groups: An Analysis of Real‐World Pharmacovigilance and Randomized Clinical Trials
Authors: Qu Han , Wu Jiaqi , Ma Cui , Qiaolongbatu Xijier , Song Xianmin , Feng Tingting , Wu Zhenghua , Lou Yuefen , Fan Guorong
Published: April 2023

Eltrombopag is clinically approved for use in immune thrombocytopenia (ITP), chronic hepatitis C‐related thrombocytopenia, and aplastic anemia and suitable for children; however, data on its overall safety profile are scarce. This study aimed to explore the clinical features of adverse drug events (ADEs) associated with eltrombopag in different age groups using individual case safety reports (ICSRs) from the World Health Organization database VigiBase and the US Food and Drug Administration Adverse Event Reporting System database from 2008 to 2022 in combination with a meta‐analysis of data from randomized clinical trials in the literature from inception to July 28, 2022. We conducted disproportionality analyses by grouping patients into the following age groups: 0–17 (0–23 months, 2–11 years, and 12–17 years), 18–64, and ≥ 65 years. The ADEs about hepatobiliary disorders, thrombosis, skin and subcutaneous tissue disorders, infections, and so on were observed more differently in each age group. Meta‐analysis results showed differences in the four system organ classes between adults and children with ITP: infections and infestations, general disorders and administration site conditions, skin and subcutaneous tissue disorders, and investigations. The adverse drug reactions in the latest version of instructions were searched in the databases to analyze their postmarketing safety signal strength. We observed signals of elevated alanine aminotransferase, aspartate aminotransferase, and blood bilirubin levels in all age groups. For children, urinary tract infection and back pain showed signals. Due to the inherent limitations of pharmacovigilance studies, more experiments are needed to assess the risks of eltrombopag in different ages.
Ammonia Synthesis via an Associative Mechanism on Alkaline Earth Metal Sites of Ca3CrN3H
Authors: Cao Yu , Toshcheva Ekaterina , Almaksoud Walid , Ahmad Rafia , Tsumori Tatsuya , Rai Rohit , Tang Ya , Cavallo Luigi , Kageyama Hiroshi , Kobayashi Yoji
Published: April 2023

Typically, transition metals are considered as the centers for the activation of dinitrogen. Here we demonstrate that the nitride hydride compound Ca3CrN3H, with robust ammonia synthesis activity, can activate dinitrogen through active sites where calcium provides the primary coordination environment. DFT calculations also reveal that an associative mechanism is favorable, distinct from the dissociative mechanism found in traditional Ru or Fe catalysts. This work shows the potential of alkaline earth metal hydride catalysts and other related 1 D hydride/electrides for ammonia synthesis.
A systematic literature review of machine learning applications in IoT
Authors: Gherbi Chirihane , Senouci Oussama , Harbi Yasmine , Medani Khedidja , Aliouat Zibouda
Published: April 2023

The Internet of Things (IoT) is a network of interconnected smart objects having capabilities that collectively form an ecosystem and enable the delivery of smart services to users. The IoT is providing several benefits into people's lives through the environment. The various applications that are run in the IoT environment offer facilities and services. The most crucial services provided by IoT applications are quick decision for efficient management. Recently, machine learning (ML) techniques have been successfully used to maximize the potential of IoT systems. This paper presents a systematic review of the literature on the integration of ML methods in the IoT. The challenges of IoT systems are split into two categories: fundamental operation and performance. We also look at how ML is assisting in the resolution of fundamental system operation challenges such as security, big data, clustering, routing, and data aggregation.