bluuebunny/crossref_metadata_2025 huggingface dataset:
Persistent Endothelial Lung Damage and Impaired Diffusion Capacity in Long COVID
Authors: Asimakos Andreas T. , Vassiliou Alice G. , Keskinidou Chrysi , Spetsioti Stavroula , Antonoglou Archontoula , Vrettou Charikleia S. , Mourelatos Panagiotis , Diamantopoulos Aristidis , Pratikaki Maria , Athanasiou Nikolaos , Jahaj Edison , Gallos Parisis , Kotanidou Anastasia , Dimopoulou Ioanna , Orfanos Stylianos E. , Katsaounou Paraskevi
Published: September 2023

Since the beginning of the pandemic, both COVID-19-associated coagulopathy biomarkers and a plethora of endothelial biomarkers have been proposed and tested as prognostic tools of severity and mortality prediction. As the pandemic is gradually being controlled, attention is now focusing on the long-term sequelae of COVID-19. In the present study, we investigated the role of endothelial activation/dysfunction in long COVID syndrome. This observational study included 68 consecutive long COVID patients and a healthy age and sex-matched control group. In both groups, we measured 13 endothelial biomarkers. Moreover, in the long COVID patients, we evaluated fatigue and dyspnea severity, lung diffusion capacity (DLCO), and the 6-min walk (6MWT) test as measures of functional capacity. Our results showed that markers of endothelial activation/dysfunction were higher in long COVID patients, and that soluble intracellular adhesion molecule 1 (sICAM-1) and soluble vascular adhesion molecule 1 (sVCAM-1) negatively correlated with lung diffusion and functional capacity (sICAM-1 vs. DLCO, r = −0.306, p = 0.018; vs. 6MWT, r = −0.263, p = 0.044; and sVCAM-1 vs. DLCO, r= −0.346, p = 0.008; vs. 6MWT, r = −0.504, p < 0.0001). In conclusion, evaluating endothelial biomarkers alongside clinical tests might yield more specific insights into the pathophysiological mechanisms of long COVID manifestations.
Non-Adherence with Physiotherapeutic Rehabilitation—A Cross-Cultural Adaption of Compliance Parameters into German
Authors: Hakam Hassan , Lettner Jonathan , Hofmann Hannes , Kersten Sebastian , Muehlensiepen Felix , Becker Roland , Prill Robert
Published: September 2023

Background: Compliance with rehabilitative physiotherapeutic measures leads to an improvement in outcomes in patients suffering from a variety of musculoskeletal conditions. To date, a tool for assessing the parameters that lead to non-adherence to physical therapy does not exist in the German language. The objective of this paper is to cross-culturally adapt a non-compliance questionnaire to German. Methods: In reference to the “Guidelines for the Process of Cross-Cultural Adaption of Self-Reported Measures”, the questionnaire was translated into German followed by a back-translation into the original language. An expert committee met and refined the pre-final version. A preliminary version was handed out to patients for evaluation of the quality of the resulting German version. Results: After the forward- and back-translation of the questionnaire, some discrepancies were discovered between the translators on the one hand and between the back-translations and the original document on the other. The statistical analysis showed satisfactory results regarding the quality of the questionnaire. Conclusion: The translation and adaption of the items proved to have a high degree of reliability. The German version will be made available for German-speaking researchers and used for evaluating a mobile-application-based physical therapy regimen by the authors of the paper.
Serum Calcium Level and Functional Atherosclerosis in Relation to Human T-Cell Leukemia Virus 1 Infection in Older Individuals
Authors: Shimizu Yuji , Yamanashi Hirotomo , Miyata Jun , Takada Midori , Noguchi Yuko , Honda Yukiko , Nonaka Fumiaki , Nakamichi Seiko , Nagata Yasuhiro , Maeda Takahiro
Published: September 2023

Serum calcium levels are known to influence vascular function. Cells infected with human T-cell leukemia virus 1 (HTLV-1) impact serum calcium levels and also affect the endothelium. Since a damaged endothelium causes functional atherosclerosis, serum calcium levels in HTLV-1 carriers may be positively associated with functional atherosclerosis. This cross-sectional study enrolled 1694 Japanese individuals aged 60 to 89 years. Functional atherosclerosis was defined as a cardio-ankle vascular index (CAVI) ≥ 9.0. Logistic regression analysis was used to evaluate the relationship between HTLV-1 carrier status and both serum calcium level and functional atherosclerosis. Even after adjusting for known confounding factors, the serum calcium level was significantly positively associated with functional atherosclerosis only in asymptomatic HTLV-1 carriers. The fully adjusted odds ratio and 95% confidence interval of functional atherosclerosis in relation to a one-standard-deviation increment of serum calcium level (0.4 mg/dL in both men and women) were 1.54 (1.18, 2.01) for HTLV-1 carriers (n = 322) and 0.99 (0.87, 1.13) for HTLV-1 non-carriers (n = 1372), respectively. The serum calcium level was positively associated with functional atherosclerosis only among older carriers of HTLV-1 infection. This finding can help effectively estimate the risk of functional atherosclerosis in asymptomatic HTLV-1 carriers.
Comparison of Performance of Micro-Computed Tomography (Micro-CT) and Synchrotron Radiation CT in Assessing Coronary Stenosis Caused by Calcified Plaques in Coronary Artery Phantoms
Authors: Ng Curtise K. C. , Sun Zhonghua , Jansen Shirley
Published: September 2023

Synchrotron-radiation-computed tomography (SRCT) allows more accurate calcified plaque and coronary stenosis assessment as a result of its superior spatial resolution; however, typical micro-computed tomography (micro-CT) systems have even higher resolution. The purpose of this study was to compare the performance of high-resolution micro-CT with SRCT in the assessment of calcified plaques and a previously published dataset of coronary stenosis assessment. This experimental study involved micro-CT scanning of three-dimensional printed coronary artery models with calcification in situ used in our previously published SRCT study on coronary stenosis assessment. Measurements of coronary stenosis utilizing both modalities were compared using a paired sample t-test. The degrees of stenosis measured on all but one micro-CT dataset were statistically significantly lower than the corresponding SRCT measurements reported in our previous paper (p < 0.0005–0.05). This indicates that the superior spatial resolution of micro-CT was able to further reduce over-estimation of stenosis caused by extensive calcification of coronary arteries and, hence, false positive results. Our results showed that the high-resolution micro-CT used in this study outperformed the Australian Synchrotron SRCT in both calcified plaque and coronary stenosis assessment. These findings will become clinically important for cardiovascular event prediction and enable reclassification of individuals with low and intermediate risk into appropriate risk categories when the technical challenges of micro-CT in clinical practice such as the small field of view and demanding on image processing power are addressed.
Virtual Reality Mindfulness and Personalized Exercise for Patients on Hemodialysis with Depressive Symptoms: A Feasibility Study
Authors: Burrows Brett T. , Morgan Ashley M. , King Alexis C. , Hernandez Rosalba , Wilund Kenneth R.
Published: September 2023

Nonadherence to exercise-related trials in hemodialysis (HD) patients is a significant burden worldwide. To address this issue, we assessed the feasibility and preliminary efficacy of a combined pre-habilitative virtual-reality-based mindfulness (VRM) program and a personalized activity prescription (PARx) in HD patients with elevated depressive symptoms. Ten HD patients (age = 59.60 ± 13.66) with elevated depressive symptoms completed a 10-week intervention. Participants were randomized into either a VRM+PARx (n = 6) or PARx alone (n = 4) group. During the 2-week prehabilitation, the VRM+PARx group completed our VRM program, while the PARx alone group received usual HD care. Post-prehabilitation, both groups began our 8-week PARx program. Feasibility was assessed by rates of recruitment, retention, adherence, and acceptability and adoption. Preliminary efficacy was measured using metrics of depressive symptoms, mindfulness, fatigue, and physical activity (PA) energy expenditure. A 25% recruitment rate was documented, with 90% retention. A 75% exercise adherence rate was observed and PARx demonstrated high perceived autonomy support (M = 27.6 ± 2.1). Post-prehabilitation, the VRM+PARx group showed significant between-group improvement in mindfulness (p = 0.02) and a significant within-group reduction in depressive symptoms (p = 0.05); however, no difference between groups was observed (p = 0.07). Post-PARx, no between-group difference was evident in PA energy expenditure; however, within the VRM+PARx group, a significant increase in PA energy expenditure was observed (p < 0.01). Fatigue remained unchanged. Our VRM and PARx programs demonstrated feasibility and potential efficacy for HD patients. However, to validate these findings, future trials should consider a larger sample size and a longer duration.
Optimization Method for Land Use of the Xi’an Rail Transit Station Area Based on a Multi-Objective Model
Authors: Tong Haiyan , Dong Xiao , Liu Jiaping
Published: September 2023

For the intensive, efficient, and sustainable utilization of land resources, it is of great significance to optimize the spatial allocation of different types of land use intensity in rail transit station areas. The current land use optimization model has some shortcomings in objective function, constraint conditions, and the solution process. In response to this, a new multi-objective optimization model for rail transit station land use was built. With station space efficiency as the starting point, the three objectives of the model optimization were the traffic volume, environment quality, and land balance of the rail transit station, and the constraint conditions were the plot ratio, environment quality, and efficiency level. Lingo was used to solve the optimal plot ratio of different types of land use intensity. Compared with the non-inferior solution of the rail transit station area multi-objective original model, the ideal plot ratio of various land uses obtained by the optimized new model was more reasonable. There was a relatively large gap between the non-inferior solutions of some original models and the actual conditions. In contrast, the optimized new model had stronger maneuverability. The deviation ranges of the two models were −0.4% to 0.9% on the residential land plot ratio adjustment index, −3.2% to 4.8% on the public land plot ratio adjustment index, and 1.1% to 1.9% on the commercial land plot ratio adjustment index. This research aimed to provide a basis and reference for the land use and planning of Xi’an rail transit station.
Place Attachment, Self-Efficacy, and Farmers’ Farmland Quality Protection Behavior: Evidence from China
Authors: Li Hao , Chen Yi , Chang Wei-Yew
Published: September 2023

Farmland pollution severely threatens humanity’s sustainable development. Exploring farmland quality protection behavior (FQPB) from the farmers’ perspective is considered one of the best ways to solve the farmland pollution problem. This study develops a theoretical framework for farmers’ FQPB from the perspectives of place attachment (consisting of place identity, dependency, and affection) and self-efficacy. We conducted a primary survey of 412 corn farmers from the northwestern Chinese province of Gansu and empirically examined the effects of place attachment and self-efficacy on farmers’ FQPB and verified the moderating effects that self-efficacy exerts on the influence of place attachment on FQPB by using hierarchical regression and propensity score matching models. The results indicate that: (1) among the three dimensions of place attachment, farmers with stronger place identity and place affection are more likely to implement FQPB; conversely, farmers who exhibit stronger place dependency are less likely to engage FQPB; and (2) self-efficacy not only effectively promotes farmers’ FQPB but also has an enhancing effect on the influence of place identity and place affection on FQPB. Our results suggest that policymakers should encourage farmers to maintain a place’s image and guide farmers to participate in place construction; thus, farmers’ place identity and place affection can be fostered. Meanwhile, the government should diversify the income sources of farmers to reduce their dependency on a single source. The finding that self-efficacy effectively promotes FQPB also implied that the formulation of farmland quality protection policies should shift from traditional command-based policies to participatory approaches, utilizing the initiative of farmers to enhance the policy’s effectiveness, which can not only promote farmers’ FQPB through self-efficacy but also strengthen the positive influence of place identity and place affection on FQPB.
Outcomes of Third-Line Trastuzumab Deruxtecan in a Patient with De Novo Stage 4 HER2-Positive Gastric Adenocarcinoma with Enteroblastic Differentiation: A Case Report
Authors: Ngan Roger Kai-Cheong
Published: September 2023

This case report describes the treatment of a patient diagnosed with de novo stage 4 human epidermal growth factor 2 (HER2)-positive gastric adenocarcinoma with enteroblastic differentiation (GAED), a rare and aggressive form of gastric cancer characterized by a tubulopapillary growth pattern and enteroblastic cell lineage markers such as GPC3, SALL4, and alpha fetoprotein. Given the patient’s symptomatic, advanced-stage cancer, treatment objectives were focused on effectively deterring disease progression and ameliorating symptoms throughout the anticipated multiple lines of therapy. Subsequent to standard first- and second-line therapies for HER2-positive metastatic GC, third-line treatment using the antibody-drug conjugate trastuzumab deruxtecan (T-DXd) for seven cycles resulted in satisfactory tumor control and well-preserved physical performance and quality of life, with minimal hematologic and pulmonary toxicities. The patient retained acceptable physical performance to receive subsequent lines of therapies, and still showed a tumor marker response to 5L trastuzumab-based chemotherapy. As the tumor was positive for both HER2 and programmed death-ligand 1 (PD-L1) expressions, the selection and sequencing of anti-HER2 and anti-PD-L1 therapies were discussed in relation to the latest U.S. Food and Drug Administration approvals and trial results.
Analytical Models for Grain Size Determination of Metallic Coatings and Machined Surface Layers Using the Four-Point Probe Method
Authors: Mehner Thomas , Lampke Thomas
Published: September 2023

The grain size of a metallic coating or the surface layer after the machining of metallic parts strongly impacts corrosion and wear properties along with fatigue behavior. By measuring the combined electrical resistance of this layer and the substrate using the four-point probe method, the grain size of the layer can be determined. For different grain shapes, models are derived based on an analytical approach. The parameters in the models can be determined by appropriate calibration measurements. As a result, the grain sizes can be determined quickly with a non-destructive method, which can be applied to ensure consistent coating or machining results as part of quality control routines in industrial processes.
Microstructure and Corrosion Behavior of Laser-Welded Al–Mn–Zr Alloy for Heat Exchanger
Authors: Lim Jeong-Min , So Yoon-Sik , Kim Jung-Gu
Published: September 2023

In this study, an Al–Mn–Zr alloy was designed and its microstructure and corrosion behavior compared after laser welding to that of AA3003. As the results of immersion and electrochemical tests showed, both alloys had a faster corrosion rate in the fusion zone than in the base metal. Laser welding caused interdendritic segregation, and spread the intermetallic compounds (IMCs) evenly throughout in the fusion zone. This increased the micro-galvanic corrosion sites and destabilized the passive film, thus increasing the corrosion rate of the fusion zone. However, Zr in the Al–Mn alloy reduced the size and number of IMCs, and minimized the micro-galvanic corrosion effect. Consequently, Al–Mn–Zr alloy has higher corrosion resistance than AA3003 even after laser welding.
Influence of Preheating Temperature on the Microstructure and Mechanical Properties of 6061/TA1 Composite Plates Fabricated by AFSD
Authors: Gong Wei , Li Yidi , Zhang Ming , Wang Hui , Liu Qinglin , Zeng Ziming , Ma Kuo , Yang Biaobiao , Lai Ruilin , Li Yunping
Published: September 2023

In this study, composite plates of 6061/TA1 were successfully manufactured using additive friction stir deposition (AFSD). The impact of preheating temperatures (room temperature, 100 °C, 200 °C) on the interfacial microstructure and interface mechanical properties at various deposition zones was studied. The results showed that as the preheating temperature increased or when the deposit zone shifted from the boundary to the center, the diffusion width of Al and Ti increased, accompanied by an increase in bonding shear strength. Moreover, in the boundary zone of the sample preheated at room temperature (P-RT), only mechanical bonding was observed, resulting in the lowest bonding shear strength. Conversely, the other samples exhibited a combination of mechanical and metallurgical bonding. Under the preheating temperature of 200 °C, interfacial intermetallic compounds were observed near the center zone, which exhibited the highest bonding shear strength.
Real-Time Nondestructive Viscosity Measurement of Soft Tissue Based on Viscoelastic Response Optical Coherence Elastography
Authors: Liu Zhixin , Liu Weidong , Chen Qi , Hu Yongzheng , Li Yurun , Zheng Xiaoya , Fang Dian , Liu Hai , Sun Cuiru
Published: September 2023

Viscoelasticity of the soft tissue is an important mechanical factor for disease diagnosis, biomaterials testing and fabrication. Here, we present a real-time and high-resolution viscoelastic response-optical coherence elastography (VisR-OCE) method based on acoustic radiation force (ARF) excitation and optical coherence tomography (OCT) imaging. The relationship between displacements induced by two sequential ARF loading—unloading and the relaxation time constant of the soft tissue—is established for the Kelvin-Voigt material. Through numerical simulation, the optimal experimental parameters are determined, and the influences of material parameters are evaluated. Virtual experimental results show that there is less than 4% fluctuation in the relaxation time constant values obtained when various Young’s modulus and Poisson’s ratios were given for simulation. The accuracy of the VisR-OCE method was validated by comparing with the tensile test. The relaxation time constant of phantoms measured by VisR-OCE differs from the tensile test result by about 3%. The proposed VisR-OCE method may provide an effective tool for quick and nondestructive viscosity testing of biological tissues.
Manufacturability-Based Design Optimization for Directed Energy Deposition Processes
Authors: Bikas Harry , Terzakis Michail Aggelos , Stavropoulos Panagiotis
Published: September 2023

Additive Manufacturing (AM) is the process of joining materials by selectively depositing them layer upon layer for the purpose of manufacturing parts or assemblies which are based on a 3D digital model. The nature of these processes results in the morphing of complex component geometries, enabling a high degree of design freedom and resulting in lightweight structures with increased performance. These processes, however, experience many limitations regarding manufacturability. The aim of this study is to develop a method and tool that optimizes the design of a component to avoid overhanging geometries and the need for supports during the Additive Manufacturing process. A workflow consisting of steps for topology optimization, orientation optimization, material addition, and machine code generation is described and implemented using Rhinoceros 3D and Grasshopper software. The proposed workflow is compared to a conventional workflow regarding manufacturing Key Performance Indicators (KPIs) such as part volume, support volume, and build time. A significant reduction is observed regarding all the KPIs by using the proposed method. Examining the results from both the conventional workflow and the proposed one, it is clear that the latter has unquestionable advantages in terms of effectiveness. In the particular case study presented, a total volume reduction of around 80% is observed. The reduction in the total volume (including the required support volume) leads to a significant reduction in the material used as well as in the build time, consequently resulting in cost reduction.
A Commemorative Special Issue in Honor of Professor Victor Nikonenko
Authors: Dammak Lasâad , Pismenskaya Natalia
Published: September 2023

Victor Nikonenko is celebrating his 70th birthday this year [...]
Particle Counting Methods Based on Microfluidic Devices
Authors: Dang Zenglin , Jiang Yuning , Su Xin , Wang Zhihao , Wang Yucheng , Sun Zhe , Zhao Zheng , Zhang Chi , Hong Yuming , Liu Zhijian
Published: September 2023

Particle counting serves as a pivotal constituent in diverse analytical domains, encompassing a broad spectrum of entities, ranging from blood cells and bacteria to viruses, droplets, bubbles, wear debris, and magnetic beads. Recent epochs have witnessed remarkable progressions in microfluidic chip technology, culminating in the proliferation and maturation of microfluidic chip-based particle counting methodologies. This paper undertakes a taxonomical elucidation of microfluidic chip-based particle counters based on the physical parameters they detect. These particle counters are classified into three categories: optical-based counters, electrical-based particle counters, and other counters. Within each category, subcategories are established to consider structural differences. Each type of counter is described not only in terms of its working principle but also the methods employed to enhance sensitivity and throughput. Additionally, an analysis of future trends related to each counter type is provided.
High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume
Authors: Duncan Josie L. , Bloomfield Mathew , Swami Nathan , Cimini Daniela , Davalos Rafael V.
Published: September 2023

Aneuploidy, or an incorrect chromosome number, is ubiquitous among cancers. Whole-genome duplication, resulting in tetraploidy, often occurs during the evolution of aneuploid tumors. Cancers that evolve through a tetraploid intermediate tend to be highly aneuploid and are associated with poor patient prognosis. The identification and enrichment of tetraploid cells from mixed populations is necessary to understand the role these cells play in cancer progression. Dielectrophoresis (DEP), a label-free electrokinetic technique, can distinguish cells based on their intracellular properties when stimulated above 10 MHz, but DEP has not been shown to distinguish tetraploid and/or aneuploid cancer cells from mixed tumor cell populations. Here, we used high-frequency DEP to distinguish cell subpopulations that differ in ploidy and nuclear size under flow conditions. We used impedance analysis to quantify the level of voltage decay at high frequencies and its impact on the DEP force acting on the cell. High-frequency DEP distinguished diploid cells from tetraploid clones due to their size and intracellular composition at frequencies above 40 MHz. Our findings demonstrate that high-frequency DEP can be a useful tool for identifying and distinguishing subpopulations with nuclear differences to determine their roles in disease progression.
A 54 µW CMOS Auto-Trimming Bandgap References (ATBGR) Achieving 90 dB PSRR for Artificial Intelligence of Things (AIoT) Chips
Authors: Poongan Balamahesn , Rajendran Jagadheswaran , Mariappan Selvakumar , Rawat Arvind Singh , Kumar Narendra , Nathan Arokia , Yarman Binboga S.
Published: September 2023

An Auto-Trimming CMOS Bandgap References Circuit (ATBGR) with PSRR enhancement circuit for Artificial Intelligence of Things (AIoT) chips is presented in this paper. The ATBGR is designed with a first-order temperature compensation technique providing a stable reference voltage of 1.25 V in the ranges of input voltages from 1.65 V to 4.5 V. An auto-trimming circuit is integrated into a PTAT resistor of BGR to minimize the influences of the process variations. The four parallel resistor pairs with PMOS switches are connected in series with the PTAT resistor. The reference voltage, VREF, is compared to an external constant value, 1.25 V, through an operational amplifier, and the output of the de-multiplexer is used to configure the PMOS switches. High power supply rejection is achieved through a PSRR enhancement circuit constituting a cascaded PMOS common gate pair. The ATBGR circuit is fabricated in 180 nm CMOS technology, consuming an area of 0.03277 mm2. The auto-trimming method yields an average temperature coefficient of 9.99 ppm/°C with temperature ranges from −40 °C to 125 °C, and a power supply rejection ratio of −90 dB at 100 MHz is obtained. The line regulation of the proposed circuit is 0.434%/V with power consumption of 54.12 µW at room temperature.
The Maternal Microbiome and Gestational Diabetes Mellitus: Cause and Effect
Authors: Dias Stephanie , Pheiffer Carmen , Adam Sumaiya
Published: September 2023

Gestational diabetes mellitus (GDM) is a growing public health concern that affects many pregnancies globally. The condition is associated with adverse maternal and neonatal outcomes including gestational hypertension, preeclampsia, placental abruption, preterm birth, stillbirth, and fetal growth restriction. In the long-term, mothers and children have an increased risk of developing metabolic diseases such as type 2 diabetes and cardiovascular disease. Accumulating evidence suggest that alterations in the maternal microbiome may play a role in the pathogenesis of GDM and adverse pregnancy outcomes. This review describes changes in the maternal microbiome during the physiological adaptations of pregnancy, GDM and adverse maternal and neonatal outcomes. Findings from this review highlight the importance of understanding the link between the maternal microbiome and GDM. Furthermore, new therapeutic approaches to prevent or better manage GDM are discussed. Further research and clinical trials are necessary to fully realize the therapeutic potential of the maternal microbiome and translate these findings into clinical practice.
Review: Effect of Experimental Diets on the Microbiome of Productive Animals
Authors: Huaiquipán Rodrigo , Quiñones John , Díaz Rommy , Velásquez Carla , Sepúlveda Gastón , Velázquez Lidiana , Paz Erwin A. , Tapia Daniela , Cancino David , Sepúlveda Néstor
Published: September 2023

The microorganisms that inhabit the gastrointestinal tract are responsible for multiple chains of reactions that affect their environment and modify the internal metabolism, their study receives the name of microbiome, which has become more relevant in recent years. In the near future, the challenges related to feeding are anticipated to escalate, encompassing the nutritional needs to sustain an overpopulated world. Therefore, it is expected that a better understanding of the interactions between microorganisms within the digestive tract will allow their modulation in order to provide an improvement in the immune system, feed efficiency or the promotion of nutritional characteristics in production animals, among others. In the present study, the main effects of experimental diets in production animals were described, emphasizing the diversity of the bacterial populations found in response to the diets, ordering them between polygastric and monogastric animals, and then describing the experimental diets used and their effect on the microorganisms. It is hoped that this study will help as a first general approach to the study of the role of the microbiome in production animals under different diets.
Biosorption of Pb(II) Using Natural and Treated Ardisia compressa K. Leaves: Simulation Framework Extended through the Application of Artificial Neural Network and Genetic Algorithm
Authors: Vázquez-Sánchez Alma Y. , Lima Eder C. , Abatal Mohamed , Tariq Rasikh , Santiago Arlette A. , Alfonso Ismeli , Aguilar Claudia , Vazquez-Olmos América R.
Published: September 2023

This study explored the effects of solution pH, biosorbent dose, contact time, and temperature on the Pb(II) biosorption process of natural and chemically treated leaves of A. compressa K. (Raw-AC and AC-OH, respectively). The results show that the surface characteristics of Raw-AC changed following alkali treatment. FT-IR analysis showed the presence of various functional groups on the surface of the biosorbent, which were binding sites for the Pb(II) biosorption. The nonlinear pseudo-second-order kinetic model was found to be the best fitted to the experimental kinetic data. Adsorption equilibrium data at pH = 2–6, biosorbents dose from 5 to 20 mg/L, and temperature from 300.15 to 333.15 K were adjusted to the Langmuir, Freundlich, and Dubinin–Radushkevich (D-R) isotherm models. The results show that the adsorption capacity was enhanced with the increase in the solution pH and diminished with the increase in the temperature and biosorbent dose. It was also found that AC-OH is more effective than Raw-AC in removing Pb(II) from aqueous solutions. This was also confirmed using artificial neural networks and genetic algorithms, where it was demonstrated that the improvement was around 57.7%. The nonlinear Langmuir isotherm model was the best fitted, and the maximum adsorption capacities of Raw-AC and AC-OH were 96 mg/g and 170 mg/g, respectively. The removal efficiency of Pb(II) was maintained approximately after three adsorption and desorption cycles using 0.5 M HCl as an eluent. This research delved into the impact of solution pH, biosorbent characteristics, and operational parameters on Pb(II) biosorption, offering valuable insights for engineering education by illustrating the practical application of fundamental chemical and kinetic principles to enhance the design and optimization of sustainable water treatment systems.
Phenolic Profiles, Antihyperglycemic, Anti-Diabetic, and Antioxidant Properties of Egyptian Sonchus oleraceus Leaves Extract: An In Vivo Study
Authors: Salim Nesrein S. , Abdel-Alim Mohamed , Said Huda E. M. , Foda Mohamed F.
Published: September 2023

This study aimed to investigate the phenolic and antioxidant properties of Egyptian Sonchus oleraceus leaves extract (SOE) while comparing the antihyperglycemic efficacy of SOE with that of conventional medicines (glibenclamide) in vivo as a substitution for insulin-deficient patients. Total phenolic (TPC) and flavonoid contents (TFC) in SOE contributed around 127.66 ± 0.56 mg GAE/gm as gallic acid equivalent (GAE) and 74.80 ± 0.55 mg QE/gm as quercetin equivalent (QE). SOE also showed significant DPPH scavenging activity at 43.46%. The presence of five phenolic and six flavonoid compounds in SOE was discovered by HPLC analysis. For the in vivo assay, 42 rats were distributed into six groups (7 Wister albino rats each). The standard control group was fed a basal diet. While the 35 rats were induced with a single dose of 100 mg kg−1 body weight (b.w.) alloxan, then treated orally with glibenclamide (GLI) at 10 mg kg−1, 100, 200, and 300 mg kg−1 SOE (positive control group) for 56 days of routine gastric oral gavages and compared to the effects of GLI, the treatment of SOE 200 and 300 mg kg−1 in diabetic rats for two months dramatically decreased blood glucose, total lipid, total cholesterol, and low-density lipoprotein cholesterol (LDLC) while boosting high-density lipoprotein cholesterol (HDLC) levels and improving liver and kidney functions. The histological assay revealed that the SOE 300 mg kg−1 treatment significantly improved the pancreas tissues, implying the potential application of Egyptian SOE as a diabetes treatment.
Effect of Exposure Concentration and Growth Conditions on the Association of Cerium Oxide Nanoparticles with Green Algae
Authors: Mackevica Aiga , Hendriks Lyndsey , Meili-Borovinskaya Olga , Baun Anders , Skjolding Lars Michael
Published: September 2023

The increasing release of engineered nanoparticles (NPs) into aquatic ecosystems makes it crucial to understand the interactions of NPs with aquatic organisms, such as algae. In this study, the association of CeO2 NPs with unicellular algae (Raphidocelis subcapitata) and changes to the cellular elemental profile were investigated using three exposure concentrations (1, 50, and 1000 µg CeO2/L) at two different algal growth conditions—exponential and inhibited growth (1% glutaraldehyde). After a 24 h-exposure, algal suspensions were settled by gravity and CeO2-NP/algae association was analyzed by single-cell inductively coupled plasma quadrupole mass spectrometry (sc-ICP-QMS) and ICP time-of-flight MS (sc-ICP-TOFMS). Concurrent detection of the cellular fingerprint with cerium indicated NP association with algae (adsorption/uptake) and changes in the cellular elemental profiles. Less than 5% of cells were associated with NPs when exposed to 1 µg/L. For 50 µg/L exposures in growing and inhibited cell treatments, 4% and 16% of cells were associated with CeO2 NPs, respectively. ICP-TOFMS analysis made it possible to exclude cellular exudates associated with CeO2 NPs due to the cellular fingerprint. Growing and inhibited cells had different elemental profile changes following exposure to CeO2 NPs—e.g., growing cells had higher Mg and lower P contents independent of CeO2 concentration compared to inhibited cells.
Migraine: Advances in the Pathogenesis and Treatment
Authors: Pleș Horia , Florian Ioan-Alexandru , Timis Teodora-Larisa , Covache-Busuioc Razvan-Adrian , Glavan Luca-Andrei , Dumitrascu David-Ioan , Popa Andrei Adrian , Bordeianu Andrei , Ciurea Alexandru Vlad
Published: September 2023

This article presents a comprehensive review on migraine, a prevalent neurological disorder characterized by chronic headaches, by focusing on their pathogenesis and treatment advances. By examining molecular markers and leveraging imaging techniques, the research identifies key mechanisms and triggers in migraine pathology, thereby improving our understanding of its pathophysiology. Special emphasis is given to the role of calcitonin gene-related peptide (CGRP) in migraine development. CGRP not only contributes to symptoms but also represents a promising therapeutic target, with inhibitors showing effectiveness in migraine management. The article further explores traditional medical treatments, scrutinizing the mechanisms, benefits, and limitations of commonly prescribed medications. This provides a segue into an analysis of emerging therapeutic strategies and their potential to enhance migraine management. Finally, the paper delves into neuromodulation as an innovative treatment modality. Clinical studies indicating its effectiveness in migraine management are reviewed, and the advantages and limitations of this technique are discussed. In summary, the article aims to enhance the understanding of migraine pathogenesis and present novel therapeutic possibilities that could revolutionize patient care.
Social Cognition Impairments in Association to Clinical, Cognitive, Mood, and Fatigue Features in Multiple Sclerosis: A Study Protocol
Authors: Doskas Triantafyllos K. , Christidi Foteini , Spiliopoulos Kanellos C. , Tsiptsios Dimitrios , Vavougios George D. , Tsiakiri Anna , Vorvolakos Theofanis , Kokkotis Christos , Iliopoulos Ioannis , Aggelousis Nikolaos , Vadikolias Konstantinos
Published: September 2023

Multiple sclerosis (MS) is a chronic immune-mediated disease of the central nervous system (CNS), characterized by the diffuse grey and white matter damage. Cognitive impairment (CI) is a frequent clinical feature in patients with MS (PwMS) that can be prevalent even in early disease stages, affecting the physical activity and active social participation of PwMS. Limited information is available regarding the influence of MS in social cognition (SC), which may occur independently from the overall neurocognitive dysfunction. In addition, the available information regarding the factors that influence SC in PwMS is limited, e.g., factors such as a patient’s physical disability, different cognitive phenotypes, mood status, fatigue. Considering that SC is an important domain of CI in MS and may contribute to subjects’ social participation and quality of life, we herein conceptualize and present the methodological design of a cross-sectional study in 100 PwMS of different disease subtypes. The study aims (a) to characterize SC impairment in PwMS in the Greek population and (b) to unveil the relationship between clinical symptoms, phenotypes of CI, mood status and fatigue in PwMS and the potential underlying impairment on tasks of SC.
Adherence to the Mediterranean Diet and Risk of Gastric Cancer: A Systematic Review and Meta-Analysis
Authors: Bai Xiao , Li Xue , Ding Siqi , Dai Dongqiu
Published: September 2023

Available results on the association between the Mediterranean diet (MD) and gastric cancer (GC) incidence are controversial. The present study aimed to determine the correlation between different subtypes of GC and MD adherence. This meta-analysis was registered on PROSPERO (CRD42021284432). We searched Embase, PubMed, Cochrane Library, and Web of Science from inception through 22 April 2023 to retrieve relevant studies. A random-effects model was used to pool odds ratios (ORs) with 95% confidence intervals (CIs). Eleven studies were included in the meta-analysis. Pooled analyses revealed that adherence to the MD was inversely associated with GC risk (ORcc, 0.43; 95% CI, 0.29 to 0.63; ORcoh, 0.84; 95% CI, 0.77 to 0.92). Higher MD adherence was significantly associated with a reduced GC risk in male (ORcc, 0.78; 95% CI, 0.65 to 0.93; ORcoh, 0.81; 95% CI, 0.65 to 1.01), but not in female (ORcc, 0.83; 95% CI, 0.68 to 1.01; ORcoh, 1.04; 95% CI, 0.82 to 1.31). Furthermore, adherence to the MD possibly decreased the risk of gastric cardia adenocarcinoma (GCA) (ORcc, 0.64; 95% CI, 0.49 to 0.83; ORcoh, 0.88; 95% CI, 0.76 to 1.02) and gastric non-cardia adenocarcinoma (GNCA) (ORcc, 0.68; 95% CI, 0.59 to 0.79; ORcoh, 0.85; 95% CI, 0.78 to 0.94). Our results indicate that adherence to the MD reduces the risk of GC and its subtypes.
A High-Fat, High-Cholesterol Diet Promotes Intestinal Inflammation by Exacerbating Gut Microbiome Dysbiosis and Bile Acid Disorders in Cholecystectomy
Authors: Xu Fusheng , Yu Zhiming , Liu Yaru , Du Ting , Yu Leilei , Tian Fengwei , Chen Wei , Zhai Qixiao
Published: September 2023

Patients with post-cholecystectomy (PC) often experience adverse gastrointestinal conditions, such as PC syndrome, colorectal cancer (CRC), and non-alcoholic fatty liver disease (NAFLD), that accumulate over time. An epidemiological survey further revealed that the risk of cholecystectomy is associated with high-fat and high-cholesterol (HFHC) dietary intake. Mounting evidence suggests that cholecystectomy is associated with disrupted gut microbial homeostasis and dysregulated bile acids (BAs) metabolism. However, the effect of an HFHC diet on gastrointestinal complications after cholecystectomy has not been elucidated. Here, we aimed to investigate the effect of an HFHC diet after cholecystectomy on the gut microbiota–BA metabolic axis and elucidate the association between this alteration and the development of intestinal inflammation. In this study, a mice cholecystectomy model was established, and the levels of IL-Iβ, TNF-α, and IL-6 in the colon were increased in mice fed an HFHC diet for 6 weeks. Analysis of fecal BA metabolism showed that an HFHC diet after cholecystectomy altered the rhythm of the BA metabolism by upregulating liver CPY7A1, CYP8B1, and BSEP and ileal ASBT mRNA expression levels, resulting in increased fecal BA levels. In addition, feeding an HFHC diet after cholecystectomy caused a significant dysbiosis of the gut microbiota, which was characterized by the enrichment of the metabolic microbiota involved in BAs; the abundance of pro-inflammatory gut microbiota and related pro-inflammatory metabolite levels was also significantly higher. In contrast, the abundance of major short-chain fatty acid (SCFA)-producing bacteria significantly decreased. Overall, our study suggests that an HFHC diet after cholecystectomy promotes intestinal inflammation by exacerbating the gut microbiome and BA metabolism dysbiosis in cholecystectomy. Our study also provides useful insights into the maintenance of intestinal health after cholecystectomy through dietary or probiotic intervention strategies.
Validity and Reliability of a Short Form of the Questionnaire for the Reflective Practice of Nursing Involving Invasive Mechanical Ventilation: A Cross-Sectional Study
Authors: Tsukuda Makoto , Fukuda Atsuko , Shogaki Junko , Miyawaki Ikuko
Published: September 2023

The number of patients on ventilators is rapidly increasing owing to the coronavirus pandemic. The previously developed Questionnaire for the Reflective Practice of Nursing Involving Invasive Mechanical Ventilation (Q-RPN-IMV) for the care of patients on ventilators includes nurses’ thought processes as items. This study aims to develop a short form of the Q-RPN-IMV for immediate use in practice and to test its reliability and validity. A convenience sample of 629 participants was used to explore the factor structure using factor analysis. The test–retest reliability was assessed using the intraclass correlation coefficient (ICC). The study was a cross-sectional design instrument development study and was reported according to GRRAS guidelines. Q-RPN-IMV short form was divided into ventilator management and patient management. The ventilator management comprised 31 items organized into six factors. Cronbach’s alpha ranged from 0.82 to 0.91, and the ICC ranged from 0.82 to 0.89. The patient management comprised 27 items organized into five factors. Cronbach’s alpha ranged from 0.75 to 0.97, and ICC ranged from 0.75 to 0.97. The Q-RPN-IMV short form is a reliable and validated instrument for assessing care for patients on ventilators. This study was not registered.
Oro-Dental Pharmacovigilance in the Digital Age: Promoting Knowledge, Awareness, and Practice in Italy through a Smart Combined System—A Conference at the 30th National Congress of the Italian College of University Professors of Dental Disciplines
Authors: La Mantia Gaetano , Buttacavoli Fortunato , Panzarella Vera , Colella Giuseppe , Capuano Annalisa , Sportiello Liberata , Parrinello Gaspare , Morreale Ilaria , Oteri Giacomo , Bellavia Giuseppe , Fusco Vittorio , Mauceri Rodolfo , Coppini Martina , Bazzano Monica , Seminara Giuseppe , Di Fede Olga , Campisi Giuseppina
Published: September 2023

Adverse drug reactions (ADRs) represent a significant threat to patients’ safety in dentistry, necessitating proactive measures for prevention and treatment. However, identifying ADRs of dental and oral interest can be challenging, and underreporting remains a persistent issue globally. This paper illustrates a smart system to help Italian healthcare personnel, including dentists, in identifying and reporting dental and oral ADRs. This educational program is within a larger multi-regional project financed by the Italian Agency of Drugs (AIFA). The proposed system comprises a free online questionnaire on ADRs of dental interest (including specific items about awareness and attitudes), and after the free consultation of an atlas of clinical images of dental and oral ADRs linked with a digital synopsis of drugs potentially related to ADRs. This section of the project was presented in April 2023 at the 30th National Congress of the Italian College of University Professors of Dental Disciplines, and the system appears to hold tremendous potential to improve the knowledge of healthcare professionals on oral and dental ADRs.
Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario
Authors: Rodríguez-Durán Arlex , Ullah Shafi , Parizi Luís Fernando , Ali Abid , da Silva Vaz Junior Itabajara
Published: September 2023

Studies evaluating candidate tick-derived proteins as anti-tick vaccines in natural hosts have been limited due to high costs. To overcome this problem, animal models are used in immunization tests. The aim of this article was to review the use of rabbits as an experimental model for the evaluation of tick-derived proteins as vaccines. A total of 57 tick proteins were tested for their immunogenic potential using rabbits as models for vaccination. The most commonly used rabbit breeds were New Zealand (73.8%), Japanese white (19%), Californians (4.8%) and Flemish lop-eared (2.4%) rabbits. Anti-tick vaccines efficacy resulted in up to 99.9%. Haemaphysalis longicornis (17.9%) and Ornithodoros moubata (12.8%) were the most common tick models in vaccination trials. Experiments with rabbits have revealed that some proteins (CoAQP, OeAQP, OeAQP1, Bm86, GST-Hl, 64TRP, serpins and voraxin) can induce immune responses against various tick species. In addition, in some cases it was possible to determine that the vaccine efficacy in rabbits was similar to that of experiments performed on natural hosts (e.g., Bm86, IrFER2, RmFER2, serpins and serine protease inhibitor). In conclusion, results showed that prior to performing anti-tick vaccination trials using natural hosts, rabbits can be used as suitable experimental models for these studies.
Recent Advances in the Control of Endoparasites in Ruminants from a Sustainable Perspective
Authors: Mendoza-de Gives Pedro , López-Arellano María Eugenia , Olmedo-Juárez Agustín , Higuera-Pierdrahita Rosa Isabel , von Son-de Fernex Elke
Published: September 2023

Consumer awareness of animal welfare and environmental health has led to a plateau level of global consumption putting serious pressure on the livestock industry [...]
BoDV-1 Infection in Children and Adolescents: A Systematic Review and Meta-Analysis
Authors: Riccò Matteo , Zanella Ilaria , Satta Elia , Ranzieri Silvia , Corrado Silvia , Marchesi Federico , Peruzzi Simona
Published: September 2023

Borna disease virus 1 (BoDV-1) can cause a severe human syndrome characterized by meningo-myeloencephalitis. The actual epidemiology of BoDV-1 remains disputed, and our study summarized prevalence data among children and adolescents (<18-year-old). Through systematic research on three databases (PubMed, EMBASE, MedRxiv), all studies, including seroprevalence rates for BoDV-1 antigens and specific antibodies, were retrieved, and their results were summarized. We identified a total of six studies for a total of 2692 subjects aged less than 18 years (351 subjects sampled for BoDV-1 antibodies and 2557 for antigens). A pooled seroprevalence of 6.09% (95% Confidence Interval [95% CI] 2.14 to 16.17) was eventually calculated for BoDV-1 targeting antibodies and 0.76% (95% CI 0.26 to 2.19) for BoDV-1 antigens. Both estimates were affected by substantial heterogeneity. Seroprevalence rates for BoDV-1 in children and adolescents suggested that a substantial circulation of the pathogen does occur, and as infants and adolescents have relatively scarce opportunities for being exposed to hosts and animal reservoirs, the potential role of unknown vectors cannot be ruled out.