bluuebunny/crossref_metadata_2025 huggingface dataset:
A quest for universal anti-SARS-CoV-2 T cell assay: systematic review, meta-analysis, and experimental validation
Authors: Binayke Akshay , Zaheer Aymaan , Vishwakarma Siddhesh , Singh Savita , Sharma Priyanka , Chandwaskar Rucha , Gosain Mudita , Raghavan Sreevatsan , Murugesan Deepika Rathna , Kshetrapal Pallavi , Thiruvengadam Ramachandran , Bhatnagar Shinjini , Pandey Anil Kumar , Garg Pramod Kumar , Awasthi Amit
Published: January 2024

Measuring SARS-CoV-2-specific T cell responses is crucial to understanding an individual’s immunity to COVID-19. However, high inter- and intra-assay variability make it difficult to define T cells as a correlate of protection against COVID-19. To address this, we performed systematic review and meta-analysis of 495 datasets from 94 original articles evaluating SARS-CoV-2-specific T cell responses using three assays – Activation Induced Marker (AIM), Intracellular Cytokine Staining (ICS), and Enzyme-Linked Immunospot (ELISPOT), and defined each assay’s quantitative range. We validated these ranges using samples from 193 SARS-CoV-2-exposed individuals. Although IFNγ ELISPOT was the preferred assay, our experimental validation suggested that it under-represented the SARS-CoV-2-specific T cell repertoire. Our data indicate that a combination of AIM and ICS or FluoroSpot assay would better represent the frequency, polyfunctionality, and compartmentalization of the antigen-specific T cell responses. Taken together, our results contribute to defining the ranges of antigen-specific T cell assays and propose a choice of assay that can be employed to better understand the cellular immune response against viral diseases.
An mRNA vaccine encoding the SARS-CoV-2 receptor-binding domain protects mice from various Omicron variants
Authors: Uraki Ryuta , Imai Masaki , Ito Mutsumi , Yamayoshi Seiya , Kiso Maki , Jounai Nao , Miyaji Kazuki , Iwatsuki-Horimoto Kiyoko , Takeshita Fumihiko , Kawaoka Yoshihiro
Published: January 2024

Here, we assessed the efficacy of a lipid nanoparticle-based mRNA vaccine candidate encoding the receptor-binding domain (LNP-mRNA-RBD) in mice. Mice immunized with LNP-mRNA-RBD based on the ancestral strain (ancestral-type LNP-mRNA-RBD) showed similar cellular responses against the ancestral strain and BA.5, but their neutralizing activity against BA.5 was lower than that against the ancestral strain. The ancestral-type LNP-mRNA-RBD protected mice from the ancestral strain or BA.5 challenge; however, its ability to reduce the viral burdens after BA.5 challenge was limited. In contrast, immunization with bivalent LNP-mRNA-RBD consisting of the ancestral-type and BA.4/5-type LNP-mRNA-RBD or monovalent BA.4/5-type LNP-mRNA-RBD elicited robust cellular responses, as well as high and moderate neutralizing titers against BA.5 and XBB.1.5, respectively. Furthermore, the vaccines containing BA.4/5-type LNP-mRNA-RBD remarkably reduced the viral burdens following BA.5 or XBB.1.5 challenge. Overall, our findings suggest that LNP-mRNA-RBD is effective against SARS-CoV-2 infection.
Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion
Authors: Schmok Jonathan C. , Jain Manya , Street Lena A. , Tankka Alex T. , Schafer Danielle , Her Hsuan-Lin , Elmsaouri Sara , Gosztyla Maya L. , Boyle Evan A. , Jagannatha Pratibha , Luo En-Ching , Kwon Ester J. , Jovanovic Marko , Yeo Gene W.
Published: January 2024

RNA-binding proteins (RBPs) modulate alternative splicing outcomes to determine isoform expression and cellular survival. To identify RBPs that directly drive alternative exon inclusion, we developed tethered function luciferase-based splicing reporters that provide rapid, scalable and robust readouts of exon inclusion changes and used these to evaluate 718 human RBPs. We performed enhanced cross-linking immunoprecipitation, RNA sequencing and affinity purification–mass spectrometry to investigate a subset of candidates with no prior association with splicing. Integrative analysis of these assays indicates surprising roles for TRNAU1AP, SCAF8 and RTCA in the modulation of hundreds of endogenous splicing events. We also leveraged our tethering assays and top candidates to identify potent and compact exon inclusion activation domains for splicing modulation applications. Using these identified domains, we engineered programmable fusion proteins that outperform current artificial splicing factors at manipulating inclusion of reporter and endogenous exons. This tethering approach characterizes the ability of RBPs to induce exon inclusion and yields new molecular parts for programmable splicing control.
Combined small-molecule treatment accelerates maturation of human pluripotent stem cell-derived neurons
Authors: Hergenreder Emiliano , Minotti Andrew P. , Zorina Yana , Oberst Polina , Zhao Zeping , Munguba Hermany , Calder Elizabeth L. , Baggiolini Arianna , Walsh Ryan M. , Liston Conor , Levitz Joshua , Garippa Ralph , Chen Shuibing , Ciceri Gabriele , Studer Lorenz
Published: January 2024

The maturation of human pluripotent stem cell (hPSC)-derived neurons mimics the protracted timing of human brain development, extending over months to years for reaching adult-like function. Prolonged in vitro maturation presents a major challenge to stem cell-based applications in modeling and treating neurological disease. Therefore, we designed a high-content imaging assay based on morphological and functional readouts in hPSC-derived cortical neurons which identified multiple compounds that drive neuronal maturation including inhibitors of lysine-specific demethylase 1 and disruptor of telomerase-like 1 and activators of calcium-dependent transcription. A cocktail of four factors, GSK2879552, EPZ-5676, N-methyl-d-aspartate and Bay K 8644, collectively termed GENtoniK, triggered maturation across all parameters tested, including synaptic density, electrophysiology and transcriptomics. Maturation effects were further validated in cortical organoids, spinal motoneurons and non-neural lineages including melanocytes and pancreatic β-cells. The effects on maturation observed across a broad range of hPSC-derived cell types indicate that some of the mechanisms controlling the timing of human maturation might be shared across lineages.
Discovery of sparse, reliable omic biomarkers with Stabl
Authors: Hédou Julien , Marić Ivana , Bellan Grégoire , Einhaus Jakob , Gaudillière Dyani K. , Ladant Francois-Xavier , Verdonk Franck , Stelzer Ina A. , Feyaerts Dorien , Tsai Amy S. , Ganio Edward A. , Sabayev Maximilian , Gillard Joshua , Amar Jonas , Cambriel Amelie , Oskotsky Tomiko T. , Roldan Alennie , Golob Jonathan L. , Sirota Marina , Bonham Thomas A. , Sato Masaki , Diop Maïgane , Durand Xavier , Angst Martin S. , Stevenson David K. , Aghaeepour Nima , Montanari Andrea , Gaudillière Brice
Published: January 2024

Adoption of high-content omic technologies in clinical studies, coupled with computational methods, has yielded an abundance of candidate biomarkers. However, translating such findings into bona fide clinical biomarkers remains challenging. To facilitate this process, we introduce Stabl, a general machine learning method that identifies a sparse, reliable set of biomarkers by integrating noise injection and a data-driven signal-to-noise threshold into multivariable predictive modeling. Evaluation of Stabl on synthetic datasets and five independent clinical studies demonstrates improved biomarker sparsity and reliability compared to commonly used sparsity-promoting regularization methods while maintaining predictive performance; it distills datasets containing 1,400–35,000 features down to 4–34 candidate biomarkers. Stabl extends to multi-omic integration tasks, enabling biological interpretation of complex predictive models, as it hones in on a shortlist of proteomic, metabolomic and cytometric events predicting labor onset, microbial biomarkers of pre-term birth and a pre-operative immune signature of post-surgical infections. Stabl is available at https://github.com/gregbellan/Stabl.
Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling
Authors: Zhang Dongdong , Ruan Jiayu , Peng Shiyu , Li Jinze , Hu Xu , Zhang Yiyi , Zhang Tianrui , Ge Yaping , Zhu Zhu , Xiao Xian , Zhu Yunxu , Li Xuzhao , Li Tingbo , Zhou Lili , Gao Qingzhu , Zheng Guoxiao , Zhao Bingrui , Li Xiangqing , Zhu Yanming , Wu Jinsong , Li Wensheng , Zhao Jingwei , Ge Woo-ping , Xu Tian , Jia Jie-Min
Published: January 2024

Neurovascular coupling (NVC) is important for brain function and its dysfunction underlies many neuropathologies. Although cell-type specificity has been implicated in NVC, how active neural information is conveyed to the targeted arterioles in the brain remains poorly understood. Here, using two-photon focal optogenetics in the mouse cerebral cortex, we demonstrate that single glutamatergic axons dilate their innervating arterioles via synaptic-like transmission between neural–arteriolar smooth muscle cell junctions (NsMJs). The presynaptic parental–daughter bouton makes dual innervations on postsynaptic dendrites and on arteriolar smooth muscle cells (aSMCs), which express many types of neuromediator receptors, including a low level of glutamate NMDA receptor subunit 1 (Grin1). Disruption of NsMJ transmission by aSMC-specific knockout of GluN1 diminished optogenetic and whisker stimulation-caused functional hyperemia. Notably, the absence of GluN1 subunit in aSMCs reduced brain atrophy following cerebral ischemia by preventing Ca2+ overload in aSMCs during arteriolar constriction caused by the ischemia-induced spreading depolarization. Our findings reveal that NsMJ transmission drives NVC and open up a new avenue for studying stroke.
A global dataset on phosphorus in agricultural soils
Authors: Ringeval Bruno , Demay Josephine , Goll Daniel S. , He Xianjin , Wang Ying-Ping , Hou Enqing , Matej Sarah , Erb Karl-Heinz , Wang Rong , Augusto Laurent , Lun Fei , Nesme Thomas , Borrelli Pasquale , Helfenstein Julian , McDowell Richard W. , Pletnyakov Peter , Pellerin Sylvain
Published: January 2024

Numerous drivers such as farming practices, erosion, land-use change, and soil biogeochemical background, determine the global spatial distribution of phosphorus (P) in agricultural soils. Here, we revised an approach published earlier (called here GPASOIL-v0), in which several global datasets describing these drivers were combined with a process model for soil P dynamics to reconstruct the past and current distribution of P in cropland and grassland soils. The objective of the present update, called GPASOIL-v1, is to incorporate recent advances in process understanding about soil inorganic P dynamics, in datasets to describe the different drivers, and in regional soil P measurements for benchmarking. We trace the impact of the update on the reconstructed soil P. After the update we estimate a global averaged inorganic labile P of 187 kgP ha−1 for cropland and 91 kgP ha−1 for grassland in 2018 for the top 0–0.3 m soil layer, but these values are sensitive to the mineralization rates chosen for the organic P pools. Uncertainty in the driver estimates lead to coefficients of variation of 0.22 and 0.54 for cropland and grassland, respectively. This work makes the methods for simulating the agricultural soil P maps more transparent and reproducible than previous estimates, and increases the confidence in the new estimates, while the evaluation against regional dataset still suggests rooms for further improvement.
High-resolution ethograms, accelerometer recordings, and behavioral time series of Japanese quail
Authors: Simian Catalina , Rossi Florencia Belén , Marin Raul Hector , Barberis Lucas , Kembro Jackelyn Melissa
Published: January 2024

Although many small vertebrates are capable of performing high-speed behaviors, most studies continue to focus on low-resolution temporal scales (>>1 s). Herein, we present video-recordings, behavior time series, and the computer software for video-analysis of Japanese quail within social groups. Home-boxes were monitored using both top and side video-cameras. High-resolution ethograms were developed for analyses. Pairs of females were assigned as either controls or using one of two methods for attachment of an accelerometer (patch or backpack). Behavior was recorded during 1 h on the first 2-days, sampled at 1 s intervals (days 1 and 2). On day 8, an unfamiliar male was placed in the home-box and its behavior was recorded during the first 10 min, sampled every 1/15 s. Male accelerometer recordings were also obtained. Video-recordings and resulting detailed high-resolution behavioral time series are valuable for reuse in comparative studies regarding the temporal dynamics of behavior within social environments. In addition, they are necessary for the assessment of novel machine learning algorithms that could be used for deciphering the output of accelerometer recordings.
Meteorological variability and predictive forecasting of atmospheric particulate pollution
Authors: Hong Wan Yun
Published: January 2024

Due to increasingly documented health effects associated with airborne particulate matter (PM), challenges in forecasting and concern about their impact on climate change, extensive research has been conducted to improve understanding of their variability and accurately forecasting them. This study shows that atmospheric PM10 concentrations in Brunei-Muara district are influenced by meteorological conditions and they contribute to the warming of the Earth’s atmosphere. PM10 predictive forecasting models based on time and meteorological parameters are successfully developed, validated and tested for prediction by multiple linear regression (MLR), random forest (RF), extreme gradient boosting (XGBoost) and artificial neural network (ANN). Incorporation of the previous day’s PM10 concentration (PM10,t-1) into the models significantly improves the models’ predictive power by 57–92%. The MLR model with PM10,t-1 variable shows the greatest capability in capturing the seasonal variability of daily PM10 (RMSE = 1.549 μg/m3; R2 = 0.984). The next day’s PM10 can be forecasted more accurately by the RF model with PM10,t-1 variable (RMSE = 5.094 μg/m3; R2 = 0.822) while the next 2 and 3 days’ PM10 can be forecasted more accurately by ANN models with PM10,t-1 variable (RMSE = 5.107 μg/m3; R2 = 0.603 and RMSE = 6.657 μg/m3; R2 = 0.504, respectively).
Complement activation products in tears of dry eye and meibomian gland dysfunction
Authors: Maehara Hiroki , Norikawa Koki , Tanaka Keiichiro , Kato Yutaka , Kasai Akihito , Mukai Ryo , Omori Tomoko , Machida Takeshi , Sekine Hideharu , Sekiryu Tetsuju
Published: January 2024

Sixty-seven patients (38 woman; median age, 69 years) were enrolled to assess complement activation products (CAPs) in tear fluid with/without dry eye (DE) and with/without meibomian gland dysfunction (MGD). Patients were divided into four groups based on the presence/absence of DE and MGD: group DM had both DE and MGD, group DN had DE without MGD, group NM had MGD without DE, and group NN had neither DE nor MGD. The levels of C3a and C5a in the collected tears were analyzed using a cytometric bead array. The C3a concentrations in the DM, DN, NM, and NN groups were 2326 pg/ml, 1411 pg/ml, 1821 pg/ml, and 978 pg/ml, respectively. The C5a concentrations in the DM, DN, NM, and NN groups were 24.7 pg/ml, 15.3 pg/ml, 24.1 pg/ml, and 12.9 pg/ml, respectively. The concentrations of C3a and C5a in the DM and NM groups were significantly higher than in the NN group (P < 0.05 for both comparisons). The CAPs in the tear fluid in MGD and DE increased. Local dysregulation of the innate immune system can be associated with the development of MGD and DE in elderly patients.
Chemical and perfusion markers as predictors of moyamoya disease progression and complication types
Authors: Kim Jae Hyun , Jeon Hanwool , Kim Moinay , Byun Joonho , Chung Yeongu , Lee Si Un , Park Wonhyoung , Park Jung Cheol , Ahn Jae Sung , Lee Seungjoo
Published: January 2024

To investigate the association between chemical markers (triglyceride, C-reactive protein (CRP), and inflammation markers) and perfusion markers (relative cerebral vascular reserve (rCVR)) with moyamoya disease progression and complication types. A total of 314 patients diagnosed with moyamoya disease were included. Triglyceride and CRP levels were assessed and categorized based on Korean guidelines for dyslipidemia and CDC/AHA guidelines, respectively. Perfusion markers were evaluated using Diamox SPECT. Cox proportional hazard analysis was performed to examine the relationship between these markers and disease progression, as well as complication types (ischemic stroke, hemorrhagic stroke, and rCVR deterioration). Elevated triglyceride levels (≥ 200) were significantly associated with higher likelihood of end-point events (HR: 2.292, CI 1.00–4.979, P = 0.03). Severe decreased rCVR findings on Diamox SPECT were also significantly associated with end-point events (HR: 3.431, CI 1.254–9.389, P = 0.02). Increased CRP levels and white blood cell (WBC) count were significantly associated with moyamoya disease progression. For hemorrhagic stroke, higher triglyceride levels were significantly associated with end-point events (HR: 5.180, CI 1.355–19.801, P = 0.02). For ischemic stroke, severe decreased rCVR findings on Diamox SPECT (HR: 5.939, CI 1.616–21.829, P < 0.01) and increased CRP levels (HR: 1.465, CI 1.009–2.127, P = 0.05) were significantly associated with end-point events. Elevated triglyceride, CRP, and inflammation markers, as well as decreased rCVR, are potential predictors of moyamoya disease progression and complication types. Further research is warranted to understand their role in disease pathophysiology and treatment strategies.
Examining double standards in layoff preferences and expectations for gender, age, and ethnicity when violating the social norm of vaccination
Authors: Moya Cristóbal , Sattler Sebastian , Taflinger Shannon , Sauer Carsten
Published: January 2024

Whether vaccination refusal is perceived as a social norm violation that affects layoff decisions has not been tested. Also unknown is whether ascribed low-status groups are subject to double standards when they violate norms, experiencing stronger sanctions in layoff preferences and expectations, and whether work performance attenuates such sanctioning. Therefore, we study layoff preferences and expectations using a discrete choice experiment within a large representative online survey in Germany (N = 12,136). Respondents chose between two employee profiles, each with information about ascribed characteristics signaling different status groups (gender, age, and ethnicity), work performance (work quality and quantity, and social skills), and whether the employees refused to vaccinate against COVID-19. We found that employees who refused vaccination were more likely to be preferred and expected to be laid off. Respondents also expected double standards regarding layoffs due to vaccination refusal, hence, harsher treatment of females and older employees. Nonetheless, their preferences did not reflect such double standards. We found little support that high work performance attenuates these sanctions and double standards, opening questions about the conditions under which social biases arise. Our results suggest detrimental consequences of vaccination refusal for individuals, the labor market, and acceptance of health policies.
An integrated approach to occupational health risk assessment of manufacturing nanomaterials using Pythagorean Fuzzy AHP and Fuzzy Inference System
Authors: Salari Samaneh , Sadeghi-Yarandi Mohsen , Golbabaei Farideh
Published: January 2024

Nanomaterials (NMs) have the potential to be hazardous owing to their unique physico-chemical properties. Therefore, the need for Health Risk Assessment (HRA) of NMs is expanding. In this study, a novel HRA was developed by the Pythagorean Fuzzy Health Risk Assessment (PFHRA) approach. Risk is considered to be the outcome of parameters including Occurrence Likelihood (OL), Potential Exposure (PE) and Toxic Effects (TE). In our proposed method, priority weights of sub-factors in Pythagorean Fuzzy-Analytical Hierarchical Process (PF-AHP) were determined by pairwise comparison based on expert judgment. After determining parameter scores, both RM and risk class (i.e., negligible, minor, major and critical) were reported as Fuzzy Inference System (FIS) output. Ultimately, a risk management strategy is presented for NMs manufacturing workplaces. This proposed method provides experts with more flexibility to express their opinions. The PFHRA approach was applied for two scenarios. The production scenario for SiNPs can create minor (5%) and major (95%) occupational health risks; the production scenario for ZnONPs can create minor (100%) concerns. However, the production SiNPs and ZnONPs utilizing the CB Nanotool technique had a major and minor risk class, respectively. The results of the present study confirmed the reliability and applicability of this approach.
Intralesional injection of tuberculin purified protein derivative (PPD) versus measles, mumps, and rubella (MMR) vaccine in treatment of molluscum contagiosum: a comparative study
Authors: Zaky Mohamed S. , Atallah Rabie B. , Mohyeldeen Aya M. Saad , Elsaie Mohamed L.
Published: January 2024

Molluscum contagiosum (MC) is a skin and mucous membrane infection caused by the molluscum virus (MCV). To evaluate safety and efficacy of intralesional injection of tuberculin purified protein derivative (PPD) antigen injection versus MMR (mumps, measles, rubella) antigen for the treatment of molluscum contagiosum (MC). A total of thirty clinically confirmed patients of molluscum were recruited for this trial. Patients who were divided into three groups (A, B and C). Each group consisted of (30) patients. Group (A) subjects received intralesional MMR injections, group (B) subjects received intralesional PPD injection and group (C) received intralesional saline injection. The results of the present study revealed complete clearance of the injected lesions in 12 patients (80%), partial response in 3 patients (20%) of group (A). In group (B), complete clearance of the treated warts was observed in 11 patients (73.3%) and partial response in 4 (26.7%) of patients. In group (C), the majority of patients 8 (53.3%) demonstrated no response while 7 (46.7%) patients showed only partial clearance. We established a good safety and efficacy profile for tuberculin PPD and MMR antigens in treatment of molluscum contagiosum.
A comparison of the disease occurrence of cerebrovascular diseases, diabetes mellitus, hypertensive diseases, and ischaemic heart diseases among hospitalized older adults in Thailand
Authors: Suanrueang Passakorn
Published: January 2024

This observational research analyzed public hospital data from the Thailand Ministry of Public Health website to investigate gender differences in four categories of non-communicable diseases (NCDs) affecting hospitalized senior Thai populations for 12 years. This study aimed to determine the cumulative effects and analyze the odds ratio (OR) according to ICD-10 cause categories for the data from 2010 to 2021, accounting for 1,327,093 cases in 2010 and 2,275,936 cases in 2021. The findings revealed statistically significant gender differences in four categories of NCDs. Men were found to be more likely than women to have two types of NCDs, as measured by the OR (95%CI): cerebrovascular diseases (OR 1.34–1.47, 95%CI 1.32–1.48), and ischaemic heart disease (OR 1.24–1.63, 95%CI 1.23–1.64). Conversely, diabetes mellitus (OR 0.64–0.84, 95%CI 0.63–0.85) and hypertensive disorders (OR 0.82–0.95, 95%CI 0.81–0.97) were discovered to have a lower likelihood of ratios related in men compared to women. However, the trend of all four NCDs in men has significantly increased every year: cerebrovascular diseases = 0.0093 year(s) + 1.3391, (R2 0.82, p-value 0.001); diabetes mellitus = 0.0171 year(s) + 0.6143, (R2 0.97, p-value 0.001); hypertension = 0.0125 year(s) + 0.8091, (R2 0.96, p-value 0.001); and ischaemic heart disease = 0.0345 year(s) + 1.1884, (R2 0.99, p-value 0.001).

Gender, a crucial biological factor, contributes to variations in the prevalence of illness. As such, it is essential to prioritize the disease risk occurrence and preventive care for men and women separately, with a focus on implementing more detailed screening and detection strategies, as well as tailored interventions.
Effects of combined extreme cold and drought stress on growth, photosynthesis, and physiological characteristics of cool-season grasses
Authors: Li Juanxia , Bai Xiaoming , Ran Fu , Zhang Caizhong , Yan Yubang , Li Ping , Chen Hui
Published: January 2024

Abiotic stress is an important factor affecting turf establishment and limiting the sustainability of the turf industry. To alleviate the effects of combined cold and drought stress in cold- and drought-prone regions, it is essential to select and introduce turfgrass germplasms that are suitable for these conditions for successful turf establishment. Thus, we evaluated the effects of combined extreme cold and drought stress on the morphological, plant leaf functional, photosynthetic, and physiological and biochemical traits of 16 wild annual bluegrass (Poa annua) germplasms. We found that there were significant differences (P < 0.05) among different provenances, combined cold and drought stress, and the main interaction factors. Combined cold and drought stress altered the morphological characteristics of the 16 germplasms to varying degrees. Furthermore, combined cold and drought stress significantly reduced the net photosynthetic rate (Pn), stomatal conductance (gs), transpiration rate (Tr), instantaneous water use efficiency (WUE), chlorophyll content, chlorophyll fluorescence parameters, accumulated intercellular CO2 concentration (Ci), and relative electrical conductivity (REC) and malondialdehyde (MDA), proline (Pro), soluble protein (SP), soluble sugar (SS), superoxide anion (O2.-), hydrogen peroxide (H2O2), and hydroxyl radical (·OH) and other active oxygen, and increased the superoxide dismutase activity (SOD), peroxidase activity (POD), catalase activity (CAT), ascorbate peroxidase activity (APX) and glutathione reductase (GR) activities. Comprehensive evaluation using principal component analysis (PCA), membership function analysis, and clustered heatmaps indicated that the ‘HZ’ germplasm had stronger combined cold and drought tolerance, whereas the ‘ZQ’ germplasm was more sensitive to combined cold and drought, which was roughly consistent with the order of morphological damage symptoms. Therefore, it is recommended to use the ‘HZ’ germplasm for planting projects in cold- and drought-prone areas, while the ‘ZQ’ germplasm is more suitable for use under warmer and non-water-deficient conditions.
miR-373 promotes invasion and metastasis of colorectal cancer cells via activating ERK/MAPK pathway
Authors: Chen Qian , Li Yunfeng , Lu Tailiang , Luo Jihui , Yang Li , Zhou Zheng , Tian Zeyu , Tan Siwen , Liu Qi
Published: January 2024

To explore the relationship between miR-373 and the occurrence and development of colorectal cancer. Additionally, it aims to predict the potential cellular signaling pathways and regulatory mechanisms in which miR-373 may be involved and provides a theoretical basis and experimental evidence for the clinical application of miR-373 as a potential biomarker, molecular target, and prognostic indicator in colorectal cancer. Real-time quantitative PCR is used to analyze the expression of miR-373 in human colorectal cancer cell lines and normal human colonic epithelial cells. Further validation of the differential expression of miR-373 in colorectal cancer cell lines is being performed. Biological functions such as cell proliferation, invasion and apoptosis are being detected by MTT, CCK-8, transwell, cell cycle analysis, and flow cytometry experiments to verify the changes in the biological behavior of colon cancer cells after overexpression and interference of miR-373 in SW-480 cells and to explore the effects of miR-373 on cell proliferation, invasion, and apoptosis in colon cancer cells. Proteomic analysis is being conducted on proteins extracted from miR-373 overexpressing SW480 cells, and mass spectrometry is used for protein identification. GO, KEGG, and enrichment analysis are being employed to analyze the significantly differentially expressed proteins. The expression levels of pathway-related proteins are being verified using Western blot. Overexpression of miR-373 increased the invasive and metastatic ability of SW-480 cells; knockdown of miR-373 decreased the invasive and metastatic ability of SW-480 cells. However, there was no statistically significant effect on cell proliferation and apoptosis in SW-480 cells. Proteomic analysis identified 78 differentially expressed proteins based on fold change (FC) > 1.2 and P < 0.05. Annotation of differentially changed proteins revealed that the MAPK signaling pathway, PI3K-Akt signaling pathway, and FAK signaling pathway may play crucial roles in the migration and invasion of colorectal cancer. Western blot analysis showed that overexpression of miR-373 significantly increased the levels of p-ERK1/2 in SW480 cells. miR-373 may activate the ERK/MAPK signaling pathway to promote the invasion and migration of colorectal cancer cells.
Striatal dopamine neurotransmission is altered in age- and region-specific manner in a Parkinson’s disease transgenic mouse
Authors: Medina-Luque Jose , Piechocinski Patrick , Feyen Paul , Sgobio Carmelo , Herms Jochen
Published: January 2024

Dopamine (DA) plays a critical role in striatal motor control. The drop in DA level within the dorsal striatum is directly associated with the appearance of motor symptoms in Parkinson’s disease (PD). The progression of the disease and inherent disruption of the DA neurotransmission has been closely related to accumulation of the synaptic protein α-synuclein. However, it is still unclear how α-synuclein affects dopaminergic terminals in different areas of dorsal striatum. Here we demonstrate that the overexpression of human α-synuclein (h-α-syn) interferes with the striatal DA neurotransmission in an age‐dependent manner, preferentially in the dorsolateral striatum (DLS) of PDGF-h-α-syn mice. While 3-month-old mice showed an increase at the onset of h-α-syn accumulation in the DLS, 12-month-old mice revealed a decrease in electrically-evoked DA release. The enhanced DA release in 3-month-old mice coincided with better performance in a behavioural task. Notably, DA amplitude alterations were also accompanied by a delay in the DA clearance independently from the animal age. Structurally, dopamine transporter (DAT) was found to be redistributed in larger DAT-positive clumps only in the DLS of 3- and 12-month-old mice. Together, our data provide new insight into the vulnerability of DLS and suggest DAT-related dysfunctionalities from the very early stages of h-α-syn accumulation.
Recent beak evolution in North American starlings after invasion
Authors: Zichello Julia M. , DeLiberto Shelagh T. , Holmes Paul , Pierwola Agnieszka A. , Werner Scott J.
Published: January 2024

European starlings are one of the most abundant and problematic avian invaders in the world. From their native range across Eurasia and North Africa, they have been introduced to every continent except Antarctica. In 160 years, starlings have expanded into different environments throughout the world, making them a powerful model for understanding rapid evolutionary change and adaptive plasticity. Here, we investigate their spatiotemporal morphological variation in North America and the native range. Our dataset includes 1217 specimens; a combination of historical museum skins and modern birds. Beak length in the native range has remained unchanged during the past 206 years, but we find beak length in North American birds is now 8% longer than birds from the native range. We discuss potential drivers of this pattern including dietary adaptation or climatic pressures. Additionally, body size in North American starlings is smaller than those from the native range, which suggests a role for selection or founder effect. Taken together, our results indicate rapid recent evolutionary change in starling morphology coincident with invasion into novel environments.
Study on micromolecular mechanical properties of C-atom reinforced SBR polymer composites
Authors: Chen Hongyue , Liu Siyuan , Fan Jingdao , Li Pengfei
Published: January 2024

A three-layer microscopic model with Fe atoms as the top and bottom layer and SBR polymer composites as the middle layer and SBR polymer composite was established and studied. By adding C atoms as reinforcement, the stability and elastic modulus and frictional coefficient changes of SBR polymer composites before and after adding C atoms were studied. In this study, the molecular dynamics method was used to change of elastic modulus was observed by stretching, compression and shear of the SBR polymer composite; The simulation shows that after adding C atom the elastic modulus of SBR polymer composite increased, the friction coefficient of polymer composite upper and lower decreases and the relative atomsic concentration, temperature, velocity, overall temperature average, kinetic energy, total energy and MSD in the thickness direction are reduced after adding C atoms. The stability of SBR polymer composites is enhanced, and the deformation under shear is weakened. In addition, it is found that the binding energy between SBR polymer composites and Fe atoms is reduced after adding C atoms.The stability of SBR polymer composites is improved during use. This work provides a method for studying the properties of rubber composites by studying the enhancement of the stability of SBR polymer composites from the microscopic point of view.
Mission-oriented determination of regional weapon maintenance demand in simulation
Authors: Zhi-gang Hu , Jing-jun Lou , bin Zeng
Published: January 2024

Determining maintenance demand ahead of mission is crucial to practical weapon maintenance, particularly to regional warship weapon maintenance. Attention is paid only to reliability, and the nature of mission or the consequence of damage is ignored while determining the regional warship weapon maintenance demand. For this reason, a method for determining regional maintenance demand based on simulation is put forward in this paper. Regional weapon maintenance system is first analyzed to build a mission-oriented maintenance demand model with the concept of mission-induced failure. Subsequently, the Anylogic platform is employed because of its advantages including agent modeling simulation and visualized process display. Four types of agent are designed for the regional maintenance system. The process of determining maintenance demand based on simulation is established on this basis. An example is eventually taken to calculate and verify the universality and effectiveness of the simulation model.
Novel betulin derivatives as multidrug reversal agents targeting P-glycoprotein
Authors: Laiolo Jerónimo , Graikioti Dafni G. , Barbieri Cecilia L. , Joray Mariana B. , Antoniou Antonia I. , Vera D. Mariano A. , Athanassopoulos Constantinos M. , Carpinella María C.
Published: January 2024

Chemotherapy is a powerful means of cancer treatment but its efficacy is compromised by the emergence of multidrug resistance (MDR), mainly linked to the efflux transporter ABCB1/P-glycoprotein (P-gp). Based on the chemical structure of betulin, identified in our previous work as an effective modulator of the P-gp function, a series of analogs were designed, synthesized and evaluated as a source of novel inhibitors. Compounds 6g and 6i inhibited rhodamine 123 efflux in the P-gp overexpressed leukemia cells, K562/Dox, at concentrations of 0.19 µM and 0.39 µM, respectively, and increased the intracellular accumulation of doxorubicin at the submicromolar concentration of 0.098 µM. Compounds 6g and 6i were able to restore the sensitivity of K562/Dox to Dox at 0.024 µM and 0.19 µM, respectively. Structure–activity relationship analysis and molecular modeling revealed important information about the structural features conferring activity. All the active compounds fitted in a specific region involving mainly transmembrane helices (TMH) 4–6 from one homologous half and TMH 7 and 12 from the other, also showing close contacts with TMH 6 and 12. Compounds that bound preferentially to another region were inactive, regardless of their free energy of binding. It should be noted that compounds 6g and 6i were devoid of toxic effects against peripheral blood mononuclear normal cells and erythrocytes. The data obtained indicates that both compounds might be proposed as scaffolds for obtaining promising P-gp inhibitors for overcoming MDR.
Diophantine imaging reveals the broken symmetry of sums of integer cubes
Authors: Sultanow Eldar , Henkel Max , Aberkane Idriss J.
Published: January 2024

We introduced a novel method for visualizing large diophantine datasets and in particular found that mapping the known integer triplets $$\{a,b,c\}$$

{
a
,
b
,
c
}

solving either equations of the type $$a^3+b^3+c^3=d$$

a
3

+

b
3

+

c
3

=
d

or $$a^3+b^3+c^3=d^3$$

a
3

+

b
3

+

c
3

=

d
3

on certain proper subgroups of the circle group exposed a very clear breaking in their symmetry and a strongly non-ergodic distribution of the solutions of sums of three cubes that had never been described before. This method could be further applied to a larger diversity of diophantine problems, informing both number-theoretical conjectures and novel methods in computer sciences on the way, along with paving the road for specific uses of machine learning in exploring diophantine datasets with possible applications in cryptography among others.
Identification of core carcinogenic elements based on the age-standardized mortality rate of lung cancer in Xuanwei Formation coal in China
Authors: Chen Zailin , Cheng Xianfeng , Wang Xingyu , Ni Shijun , Yu Qiulian , Hu Junchun
Published: January 2024

In this study, the core carcinogenic elements in Xuanwei Formation coal were identified. Thirty-one samples were collected based on the age-standardized mortality rate (ASMR) of lung cancer; Si, V, Cr, Co, Ni, As, Mo, Cd, Sb, Pb, and rare earth elements and yttrium (REYs) were analyzed and compared; multivariate statistical analyses (CA, PCA, and FDA) were performed; and comprehensive identification was carried out by combining multivariate statistical analyses with toxicology and mineralogy. The final results indicated that (1) the high-concentration Si, Ni, V, Cr, Co, and Cd in coal may have some potential carcinogenic risk. (2) The concentrations of Cr, Ni, As, Mo, Cd, and Pb meet the zoning characteristics of the ASMR, while the Si concentration is not completely consistent. (3) The REY distribution pattern in Longtan Formation coal is lower than that in Xuanwei Formation coal, indicating that the materials of these elements in coal are different. (5) The heatmap divides the sampling sites into two clusters and subtypes in accordance with carcinogenic zoning based on the ASMR. (6) PC1, PC2, and PC3 explain 62.629% of the total variance, identifying Co, Ni, As, Cd, Mo, Cr, and V. (7) Fisher discriminant analysis identifies Ni, Si, Cd, As, and Co based on the discriminant function. (8) Comprehensive identification reveals that Ni is the primary carcinogenic element, followed by Co, Cd, and Si in combination with toxicology. (9) The paragenesis of Si (nanoquartz), Ni, Co, and Cd is an interesting finding. In other words, carcinogenic elements Ni, Co, Cd, and Si and their paragenetic properties should receive more attention.
Reparation of nano-FeS by ultrasonic precipitation for treatment of acidic chromium-containing wastewater
Authors: Dai Mengjia , Di Junzhen , Zhang Ting , Li Tuoda , Dong Yanrong , Bao Sihang , Fu Saiou
Published: January 2024

Nano-FeS is prone to agglomeration in the treatment of chromium-containing wastewater, and ultrasonic precipitation was used to synthesize nano-FeS to increase its dispersion. The optimization of the preparation method was carried out by single factor method (reaction temperature, Fe/S molar ratio and FeSO4 dropping flow rate) and response surface methodology. Dynamic experiments were constructed to investigate the long-term remediation effect and water column changes of nano-FeS and its solid particles. The changes of the remediation materials before and after the reaction were observed by SEM, and the mechanism of the remediation of chromium-containing wastewater by nano-FeS prepared by ultrasonication was revealed by XRD. The results showed that the reaction temperature of 12 °C, Fe/S molar ratio of 3.5 and FeSO4 dropping flow rate of 0.5 mL/s were the best parameters for the preparation of nano-FeS. The nano-FeS has efficient dispersion and well-defined mesoporous structure in the form of needles and whiskers of 40–80 nm. The dynamic experiments showed that the average removal of Cr(VI) and total chromium by nano-FeS and its immobilized particles were 94.97% and 63.51%, 94.93% and 45.76%, respectively. Fe2+ and S2− ionized by the FeS nanoparticles rapidly reduced Cr(VI) to Cr(III). Part of S2− may reduce Fe3+ to Fe2+, forming a small iron cycle that gradually decreases with the ion concentration. Cr(III) and Fe2+ form Cr(OH)3 and FeOOH, respectively, with the change of aqueous environment. Another part of S2− reacts with Cr(III) to form Cr2S3 precipitate or is oxidized to singlet sulfur. The FeS nanoparticles change from short rod-shaped to spherical shape. Compared with the conventional chemical precipitation method, the method used in this study is simple, low cost, small particle size and high removal rate per unit.
Prevalence, incidence of and risk factors for vertebral fracture in the community: the Vietnam Osteoporosis Study
Authors: Nguyen Hoa T. , Nguyen Bao T. , Thai Thi H. Nhung , Tran An V. , Nguyen Tan T. , Vo Tam , Mai Linh D. , Tran Thach S. , Nguyen Tuan V. , Ho-Pham Lan T.
Published: January 2024

The epidemiology of vertebral fractures (VF) in underrepresented populations is not well-documented. This cohort study was part of a longitudinal osteoporosis research project with the aim of determining the prevalence, incidence, and risk factors for VF. 401 individuals (155 men) aged 50 years and older without a clinical diagnosis of VF were took radiographs at baseline and 2 years later. VF were ascertained using the Genant's semi-quantitative method. Bone mineral density (BMD) of femoral neck and lumbar spine were measured by dual-energy X-ray absorptiometry (Hologic Inc). The association between VF and risk factors was analyzed by the multiple logistic regression. The 95% confidence interval for prevalence and incidence was estimated by exact Poisson test. At baseline, the prevalence of VF was 12.2% (n = 49, 95% CI 9.0–16.2%) and increased with advancing age with one-fifth of those aged 70 and older having a VF. During the follow-up period, we observed 6 new VF, making the incidence of 6.6/1000 person-years (n = 6, 95% CI 2.4–14.3). The risk of prevalent VF was associated with male gender (OR: 2.67; 95% CI 1.28–5.87) and T-score at the femoral neck (OR per one SD decrease: 1.1; 1.03–1.17). These data indicate that VF is common among adults, and that lower femoral neck BMD was a risk factor for VF.
Association of exposure to indoor molds and dampness with allergic diseases at water-damaged dwellings in Korea
Authors: Lee Seokwon , Ryu Seung-Hun , Sul Woo Jun , Kim Seunghyun , Kim Dohyeong , Seo SungChul
Published: January 2024

This study aims to characterize levels of molds, bacteria, and environmental pollutants, identify the associations between indoor mold and dampness exposures and childhood allergic diseases, including asthma, allergic rhinitis, atopic dermatitis, using three different exposure assessment tools. A total of 50 children with their parents who registered in Seoul and Gyeonggi-do in Korea participated in this study. We collated the information on demographic and housing characteristics, environmental conditions, and lifestyle factors using the Korean version of the International Study of Asthma and Allergies in Childhood questionnaire. We also collected environmental monitoring samples of airborne molds and bacteria, total volatile organic compounds, formaldehyde, and particulate matter less than 10 µm. We evaluated and determined water damage, hidden dampness, and mold growth in dwellings using an infrared (IR) thermal camera and field inspection. Univariate and multivariate regression analyses were performed to evaluate the associations between prevalent allergic diseases and exposure to indoor mold and dampness. Indoor mold and bacterial levels were related to the presence of water damage in dwellings, and the mean levels of indoor molds (93.4 ± 73.5 CFU/m3) and bacteria (221.5 ± 124.2 CFU/m3) in water-damaged homes were significantly higher than those for molds (82.0 ± 58.7 CFU/m3) and for bacteria (152.7 ± 82.1 CFU/m3) in non-damaged dwellings (p < 0.05). The crude odds ratios (ORs) of atopic dermatitis were associated with < 6th floor (OR = 3.80), and higher indoor mold (OR = 6.42) and bacterial levels (OR = 6.00). The crude ORs of allergic diseases, defined as a group of cases who ever suffered from two out of three allergic diseases, e.g., asthma and allergic rhinitis, and allergic rhinitis were also increased by 3.8 and 9.3 times as large, respectively, with water damage (+) determined by IR camera (p < 0.05). The adjusted OR of allergic rhinitis was significantly elevated by 10.4 times in the water-damaged dwellings after adjusting age, sex, and secondhand smoke. Therefore, a longitudinal study is needed to characterize dominant mold species using DNA/RNA-based sequencing techniques and identify a causal relationship between mold exposure and allergic diseases in the future.
One-pot synthesis of novel chitosan-salicylaldehyde polymer composites for ammonia sensing
Authors: Saeed Ahmed Muhammed , Taha Ahmed Gaber , Dardeer Hemat Mohamed , Aly Moustafa Fawzy
Published: January 2024

Chitosan (Chs)-salicylaldehyde (Sal) polymer derivatives were formed via the reaction of Chs-Sal with zinc oxide nanoparticles (ZnO NPs) and beta-cyclodextrin (β-CD). These polymers were synthesized through inclusion with β-CD and doping with ZnO NPs to give pseudopolyrotaxane and Chs-Sal/ZnO NPs composite, respectively, for low-temperature detection and sensing of NH3 vapors as great significance in environmental control and human health. Additionally, the polymer (Chs-Sal/β-CD/ZnO NPs) was prepared via the insertion of generated composite (Chs-Sal/ZnO NPs) through β-cyclodextrin ring. The structural and morphological characterizations of the synthesized derivatives were confirmed by utilizing FTIR, XRD and, SEM, respectively. Also, the optical properties and thermal gravimetric analysis (TGA) of the synthesized polymers were explored. The obtained results confirmed that using β-CD or ZnO NPs for modification of polymer (Chs-Sal) dramatically enhanced thermal stability and optical features of the synthesized polymers. Investigations on the NH3-sensing properties of Chs-Sal/β-CD/ZnO NPs composite were carried out at concentrations down to 10 ppm and good response and recovery times (650 s and 350 s, respectively) at room temperature (RT) and indicated that modification by β-CD and doping with ZnO NPs effectively improves the NH3-sensing response of Chs-Sal from 712 to 6192 using Chs-Sal/β-CD/ZnO NPs, respectively, with low LOD and LOQ of 0.12 and 0.4 ppb, respectively.