bluuebunny/crossref_metadata_2025 huggingface dataset:
UNSAFE ACT AS THE RISK FACTORS OF ACCIDENTS IN USING MILL IN ROOF TILE INDUSTRY AT PEJATEN VILLAGE TABANAN REGENCY
Authors: Sutjana D.P. , Adiputra N.
Published: December 2022

The use of mill and hand press machines had been increased the productivity ofroof tile home industry at Pejaten Village Tabanan Regency. But the increased ofproductivity followed by increased of working accidents. Many effort had been done toreduced the accident such as equipped the mill machine with machineâ€™s cover, footprotective devices, and training to the new employees; but, the accident still happen.Although the incident of accident was lower but the disability was severed such as loss ofhand, loss oh finger, or other physical defects of the body part. Such physical defectscaused a serious effect on workerâ€™s daily lives and social activities. To find out the unsafeacts as the risk factors of accident, a retrospective study was done using interview and onthe spot observation to the milling process. The results are found: there are three riskfactors contributed to the accidents, namely: the human factor, the tools and theenvironment. The human factor contributed highest one for the accident. From humanfactors it was found that there are many unsafe acts, such as: careless, going up to theupper inlet of mill to push mixing raw material with foot or hand, moving the millwithout turn off the machine, cleaning the mill without turn off the mill, taking out thestone which trapped the rollers using hand, operating the machine without guarding,working with tired condition, working without knowing the operation procedure, withouttraining, improver lighting, working without personal protective devices, etc. The highestincidents come from pushing the raw materials on inlet of mill in order to speed-up themilling process. To solve the problems some actions must be done such as providing themill with the appropriate equipments; by doing so, the operator can push the materialsafely to speed-up the mill process, turn off the machine before cleaning, appropriatetraining before operating of mill, using the personal protective devices, and doing goodhouse keeping and inspection, regularly.
REGULATION OF SHIFT WORK TO AVOID SHACK OUT OF EMPLOYEES IN HOTEL.
Authors: Sutjana D.P.
Published: December 2022

Since the tragedy of bomb blast at Legian Kuta and SARS epidemictogether with travel warning from foreign country resulting a stepdecreasng of tourist visit to Bali. The Decreasing number of touristvisit cause of tourism stake holders facing many problem such as: emtyroom of hotel, restaurant, travel bureau, small scakle industries. Manyof them close their activities and sack out their employees. But sometwo star hotel in Sanur treid to manage their own way inorder toregulate their employees jog to maintain hotel operational without sackout their employees. The study has been done to find out how to manageemployees shift work in those hotel. The result shown of those 4 hotelat Sanur: 1) one of them gave the employees for 15 days work and 15 daysoff with 50% salary, 2) the second hotel gave one week of and three weekwark with 30% salary off, 3) the third full work, salary as usual butemployees take over hotel renovation job, 4)the employees work as usualwith full salary but laundry work take over by employees themselves.With those management system the hotel operational cost can be minimizedso the dayly operation can be maintain with out shack out theiremployees.
CULTURAL APPROACH TO REDUCED TRAFFIC ACCIDENT IN BALI
Authors: Sutjana I.D.P.
Published: December 2022

Safety on traveling is needed by all travelers. Safety on traveling is determined bymany factors such as the drivers, vehicles, road, environment and it relation each other.Balinese culture which influence by Hinduism, believe that traffics accidents occurred asa result of not harmonize the relation between human as a driver to vehicle or car, humanto the god and human to the environment. So in Balinese daily of life there is an effort toharmonize the relation of all components using cultural approach. In doing that manyactivities such as doing an offering to the new or after reparation of car, to offer sajen inthe car everyday during traveler, every 210 days during â€œ tumpek landep dayâ€ aftercleaning the car offer sajen, ring the bell or offer sajen during passing the sacral place ortemple, offer sajen and look after during night where the accident were happen, offersajen (mlaspas) the new road, forbidden to travel during wedding ceremony or get familydied. These activities mean to making the tools more humane so that the tools harmonizeto the user, forgive to the magic spirit around the road. By practicing the culturalapproach the traffic accident at many places can be reduced. One taxi company shownthat annually maintenance cost lower compared than the same company in Java.
COMPARISON STUDY IN USING PLOUGH, TRACTOR AND CULTIVATOR FOR RICE FIELDâ€™ LAND PREPARATION
Authors: Sutjana I.D.P. , Adiputra I Nyoman
Published: December 2022

Land preparation of the rice field is one of the most important steps for paddyplantation. Formerly plough is one of the main tools for land preparation, but now thefarmer used tractor or cultivator. From the farmer experience who are used that tools forland preparation there had advantages and disadvantages. The used of plough for landpreparation for long time ago the deep of rice field soil is constant because the ploughblade can be adjusted up and down. The plough can be turn to the right or left side so thatall part of rice field except the corner can be cultivate. But for pulling the plough must bedifficult to find two cows. While if using tractor or cultivator the right or left side and thecorner of the rice field canâ€™t be cultivate. Deep of rice filed soil become shallow so thefertility of soil reduced. Beside that using tractor or cultivator for land preparation needmore fuel. The plough were easy to carry out, but tractor and cultivator difficult to carryout because the weight more that 200 kg.
RESPON KEKEBALAN HUMORAL MENCIT BALB/C YANG DIVAKSINASI DENGAN VAKSIN LIMPA DAN VAKSIN KULTUR PENYAKIT JEMBRANA TERHADAP PROTEIN VIRUS JEMBRANA
Authors: MANIK WIDIYANTI NI LUH PUTU , SUATA I KETUT , MANTIK ASTAWA I NYOMAN , - HARTANINGSIH
Published: December 2022

Sapi bali adalah salah satu aset nasional Indonesia yang harus dilestarikan karena mempunyai keuntungan ekonomi. Tetapi sapi bali mempunyai beberapa kelemahan penyakit khususnya penyakit Jembrana yang disebabkan oleh virus penyakit Jembrana (JDV). Pencegahan terhadap penyakit Jembrana telah dilakukan dengan vaksinasi. Vaksin yang terbukti dapat menurunkan tingkat kematian sapi bali terserang JDV adalah vaksin limpa. Jenis vaksin ini hanya mampu menginduksi kekebalan dengan perlindungan 70%. Proteksi ini dapat ditingkatkan jika jumlah virus yang digunakan dalam vaksin meningkat. Tekhnik kultur in vitro adalah salah satu metode meningkatkan jumlah virus penyakit Jembrana, dan selanjutnya dibuat vaksin kultur. Hasil penelitian menunjukkan bahwa sel limfosit sapi bali terinfeksi JDV adalah 9,5% pada limpa dan 57,43% pada sel kultur. Uji westernimmunoblotting sel limfosit sapi bali dari darah tepi dan limpa terinfeksi JDV menggunakan antibodi monoklonal (AbMo) anti Ca, terdeteksi protein dengan berat molekul 26 kDa, 42 kDa dan 51 kDa. Pada medium kultur PBMC dan endapan plasma sapi bali terinfeksi JDV, teridentifikasi protein dengan berat molekul 16 kDa an 26 kDa menggunakan AbMo, dan teridentifikasi protein dengan berat molekul 16 kDa; 21,5 kDa. 26 kDa; 29,7 kDa; 40 kDa dan 50 kDa menggunakan AbPo. Uji Elisa didapatkan nilai absorban antibodi mencit balb/c yang divaksinasi dengan vaksin kultur penyakit Jembrana lebih tinggi yaitu sebesar 0,3089 dibandingkan vaksin limpa yaitu sebesar 0,177 dengan p<0,05. Nilai absorban antibodi mencit balb/c terhadap antigen Ca, SU dan tat, memperlihatkan nilai absorban terhadap antigen SU berbeda sangat signifikan dibandingkan dengan antigen Ca dan antigen tat (p<0,01). Antigen Ca berbeda signifikan terhadap antigen tat (p<0,05).
FRAKSI HEKSAN EKSTRAK BIJI PEPAYA MUDA DAPAT MENGHAMBAT PROSES SPERMATOGENESIS MENCIT JANTAN LEBIH BESAR DARIPADA FRAKSI METANOL EKSTRAK BIJI PAPAYA MUDA
Authors: Satriyasa Bagus Komang
Published: December 2022

Fraksi ekstrak heksan mengandung dua golongan zat aktif yang bersifat antifertilitas yaitu golongan steroid dan golongan triterpenoid yang diperkirakan bersifat antifertilitas, walupun mekanisme kerjanya belum jelas. Rancangan penelitian yang digunakan ialah â€˜â€™ Pre-test post-test control group designâ€™â€™. Penelitian ini memakai 30 ekor mencit jantan strain balb C , umur sekitar 12 minggu dengan berat 20-22 gram, kemudian dikelompokkan secara random menjadi 3 kelompok yang masing-masing terdiri dari 10 ekor. Satu kelompok kontrol (P0 = yang diberikan aquabides), dan dua kelompok perlakuan (P1 = kelompok perlakuan yang diberikan fraksi heksan ekstrak 20 mg/20 gram/hari, P2 = kelompok perlakuan yang diberikan fraksi ekstrak metanol 20 mg/20 gram/hari). Setelah 36 hari perlakuan lalu dilakukan pemeriksaan testis dan darah mencit. Data yang diperoleh dianalisis secara statistik dengan menggunakan uji normalitas Kolmogorov Smirnov Goodnees of Fit test, uji homogenitas, dan uji anova. Didapatkan hasil bahwa fraksi heksan ekstrak maupun metanol dapat menurunkan jumlah sel spermatogonia A, sel spermatosit primer pakhiten, sel spermatid, dan sel Sertoli secara sangat bermakna (p < 0,01), sedangkan jumlah sel Leydig dan kadar hormon testosteron menurun tidak bermakna (p > 0,05). Dari hasil penelitian ini dapat disimpulkan bahwa fraksi heksan ekstrak biji pepaya dapat menurunkan jumlah rata-rata sel spermatogonia A, spermatosit primer pakiten, spermatid, sel Sertoli, sel Leydig dan kadar hormon testosteron lebih besar dari pada fraksi metanol ekstrak biji pepaya muda.
REDESIGN OF WORK PLACE AND SYSTEM WITH ERGONOMIC INTERVENTION IMPROVES THE PERFORMANCE OF THE SKT (HAND ROLLED CIGARETTE) ROLLERS AT CIGARETTE INDUSTRY â€œXâ€ IN KEDIRI EAST JAVA
Authors: Sajiyo H , Manuaba Adnyana , Adiputra Nyoman , Sutjana Putu
Published: December 2022

In this research ergonomic intervention of the 4 main factors influencingperformance was conducted. The four main factors are : (a) intervention of humancovering the change in position of the SKT rollers from the position of facing oneanother into that of facing the same direction, from the hunchbacked sitting positioninto the upright one; (b) intervention of work equipment covering the changes inform, dimension, table and chair size, and layouts of space and work facilities ; (c)intervention of work system covering the changes in work process arrangement, rightand left hand movement, time allocated for active break and in the removal oftobacco which used to be done by the knockers but now is done by the rollers; (d)intervention of work environment, that is, exhaust installation to regulate thecirculation and wind humidity in the SKT rollersâ€™ room. Redesign of place and worksystem with ergonomic intervention contribute to : (a) the decrease in work loadfrom medium category into light category; (b) the decrease in work tiredness frombeing very tired category into being not tired; (c) the decrease in musculoskeletalcomplaint from high category (being disturbed) into light category (beingundisturbed); (d) the decrease in medical treatment cost by 56,97%; (e) the increasein work productivity by 41,47%; (f) the increase in the SKT rollersâ€™ salaries by15,10%; and (g) the increase in the companyâ€™s profit by 12,76%. Generally, redesignof place and work system can increase the performance of the SKT rollers at cigaretteindustry â€œXâ€ at Kediri, East Java. Therefore, it has been suggested that the companycontinue to redesign the place and work system with ergonomic intervention in theother units.
MODEL AKTIVITAS PRAKTIKUM LAPANGAN BERBASIS ERGONOMI (APeLErg) MEMPERBAIKI RESPON FISIOLOGIS TUBUH, MENURUNKAN KELELAHAN, DAN MENINGKATKAN KINERJA, DIBANDINGKAN DENGAN MODEL LAMA (APeL), PADA MAHASISWA FMIPA UNIMA
Authors: Palilingan Rolles , Manuaba Adnyana , Adiputra Nyoman , Pangkahila Alex
Published: December 2022

Aktivitas praktikum lapangan merupakan kegiatan yang dilakukan sebagaiimplementasi kurikulum akademik di Jurusan Fisika FMIPA UNIMA. Sebagaiimplementasi kurikulum tersebut telah dibuat model aktivitas praktikum lapangan (modelAPeL) dan telah digunakan sejak tahun 2001. Namun model APeL ternyata menimbulkanrisiko yang merugikan bagi mahasiswa dilihat dari respon fisiologis dan kelelahansehingga mahasiswa belum dapat mencapai kinerja yang diharapkan. Untuk itu telahdiupayakan dengan penerapan pendekatan ergonomi total (PET) suatu model baru yaitumodel APeLErg. Untuk menguji keandalan model APeLErg dibandingkan dengan modelAPeL, telah dilakukan penelitian dengan hipotesis: model APeLErg, dibandingkan denganmodel APeL; memperbaiki respon fisiologis tubuh; menurunkan kelelahan; danmeningkatkan kinerja mahasiswa di daerah dataran rendah/panas dan di daerah datarantinggi/dingin. Penelitian ini dilakukan dalam dua tahap dengan menggunakan rancangansama subjek. Penelitian pada tahap pertama dilakukan di daerah panas denganmenggunakan 15 orang subjek sedangkan penelitian tahap kedua di daerah dinginmenggunakan 18 orang subjek. Hasil penelitian tahap I dan tahap II menunjukkan bahwaaktivitas dengan model APeLErg dapat: memperbaiki respon fisiologis mahasiswa secarasignifikan (p<0,05); menurunkan rata-rata skor kelelahan umum secara signifikan(p<0,05); meningkatkan kecepatan, kekonstanan dan ketelitian mahasiswa secarasignifikan (p<0,05); dan meningkatkan kinerja mahasiswa secara signifikan (p<0,05). Darihasil penelitian ini dapat disimpulkan bahwa model APeLErg dapat: memperbaiki responfisiologis mahasiswa; menurunkan tingkat kelelahan mahasiswa; dan meningkatkankinerja mahasiswa dalam melakukan aktivitas praktikum lapangan.
THE ROLE OF INTENSIVE INSULIN THERAPY ON SUPEROXIDE DISMUTASE (SOD), TUMOR NECROSIS FACTOR-? (TNF-?), AND INTERLEUKIN-6 (IL-6) ON HYPERGLYCEMIA IN CRITICALLY ILL PATIENTS
Authors: Wiryana Made , Suastika Ketut , Bagianto Hari , Bakta Made
Published: December 2022

Hyperglycemia and insulin resistance are common in critically ill patientsin the ICU, although they have not previously had diabetes. It has been reportedthat pronounced hyperglycemia may lead to complications in such patients, andcause the reactive oxygen species (ROS) production, although controlled trial dataare still lacking. The current debatable issue, focusing on whether the intensiveinsulin therapy, aimed at normalizing blood glucose, may improvepatientsâ€™prognosis. Then, the debate is mainly about the time to start the therapy,and target of blood glucose level. Therefore, this research is mainly designed andaimed at knowing the difference between intensive insulin therapy andconventional insulin therapy on the increase of superoxide dismutase (SOD),decrease of cytokine production (TNF-? and IL-6), increase of albumin level, andevent of SIRSThis study was carried out in a randomly pre and post-test control groupdesign, involving 40 adult patients being nursed through the ICU Sanglah hospitalDenpasar. They were randomly assigned to receive intensive insulin therapy, inwhich blood glucose was decreased and maintained at the level between 80-110mg/dl, or conventional insulin therapy in which the insulin was infused only if theblood glucose level exceeded 215 mg/dl, decreased and maintained then at thelevel between 180-200 mg/dl.The result of the study showed that there was (1) significant increase ofSOD mean level (370. 70 vs 98.50 U/gHb, p=0.001); (2) no significant decreaseof TNF-? mean level; (3) significant decrease of IL-6 mean level (10.26 vs 2.25;p=0.023); (4) significant increase of albumin mean level ( 0.62 vs 0.22); (5)significant decrease of SIRS (10 % vs 40 %, p=0.000) on intensive insulin therapygroup compared to conventional insulin therapy group. It can be concluded thatintensive insulin therapy could maintain blood glucose level between 80 â€“ 110mg/dl, increase SOD level, decrease IL-6 level, increase albumin level, anddecrease SIRS on hyperglycemia in critically ill ICU patients.
HIGH SERUM CONCENTRATION OF INTERLEUKINE-6 AND RANK-LIGAND AS RISK FACTORS FOR OSTEOPOROSIS IN ESTROGEN DEFICIENCY POST-MENOPAUSAL WOMEN
Authors: Siki Kawiyana Ketut , Surya Gusti Putu , Bakta Made , Roeshadi Djoko
Published: December 2022

Osteoporosis in post-menopausal women is not merely due to deficient estrogenhormone production. The development of osteoporosis is due to increased boneresorption by osteoclasts. The osteoclastâ€™s number and activity is controlled by activatingfactors such as IL-6 and RANK-L. The objective of this study was to determine that highIL-6 and RANK-L serum concentrations are risks for osteoporosis in estrogen deficientpost-menopausal women. The serum concentration of ÃŸ-CrossLaps (CTx) was measuredto determine bone resorption rate. This is an observational analytical study using case andcontrol design conducted at Sanglah General Hospital of Denpasar. The sample size wascalculated using the paired case-control study formula. There were 41 osteoporotic and41 non-osteoporotic (control) estrogen deficient post-menopausal women involved in thestudy.Data were analyzed by using the t-paired and McNemar tests. Mean serumconcentration of IL-6 among the osteoporotic women was significantly higher ascompared to that of the controls (3.47Â±1.75 pg/ml vs 2.51Â±1.13 pg/ml, p = 0.001). Meanserum concentration of RANK-L among the osteoporotic women was also significantlyhigher as compared to that of the controls (320.66Â±122.44ng/ml vs 249.94Â±82.41 ng/ml,p = 0.002). To qualify as risk factors for osteoporosis, the cut-off point for IL-6 was 2.17pg/ml (OR = 4, CI 95%: 1.23-14.24; p = 0.032); the cut-off point for RANK-L was275.165 ng/ml (OR = 8, CI 95%: 1.84-34.79; p = 0.001). Analysis of both high serumconcentration of IL-6 and RANK-L was associated with an odd ratio of 9 (CI 95%: 4,27-18,96, p=0,000). CTx concentration in the osteoporotic women was significantly higherthan in the controls (0.60Â±0.22ng/ml vs 0.46Â±0.16ng/ml, p = 0.004).We found that the high IL-6 and RANK-L serum concentrations were risk factorsin estrogen deficient post-menopausal women. CTx being a marker for osteoclastic boneresorption activity, increased in concentration higher in osteoporotic than in nonosteoporoticwomen. The high serum concentrations of IL-6 and RANK-L could be usedas predictors for osteoporosis in estrogen deficient post-menopausal women.
LIPID PEROXIDATION AFTER INTRACORTICAL INJECTION OF FERRIC CHLORIDE INCREASES THE INCIDENCE OF SEIZURES IN YOUNG RATS
Authors: Golden Nyoman , Agus Bagiada Nyoman , Darmadipura Sajid
Published: December 2022

Clinical studies have shown that the incidence of early posttraumatic seizures ishigher in children than in adults and it has been proposed that iron-induced lipidperoxidation has an important role in the development of epileptogenic foci. In this study,we examined some of the hypothesized reasons for the difference in the incidence ofearly posttraumatic seizures between young and adult rats. Twelve young and twelveadult rats were randomized into 4 groups. Group 1 and 2 were control groups, eachcomprising of 6 young rats and 6 adult rats respectively and were given intracorticalinjections of normal saline. Group 3 and 4 were injury groups, again comprising 6 youngrats and 6 adult rats respectively and were given intracortical injections of FeCl3. All ratswere observed for 6 hours post injection for the occurrence of seizures and were thenkilled. The injected hemispheres were extirpated and tested for malondialdehyde (MDA)level and superoxide dismutase (SOD) activity as indices of oxidative damage. Resultsshowed that seizures were observed only in Group 3. Increased MDA level and decreasedSOD activity were observed in Group 3 (ANOVA, p<0.001). Increased MDA levels anddecreased SOD activity were significantly higher in rats with seizures (Group 3) than inthose without seizures (independent t-test, p<0.001). We conclude was that differentlevels of lipid peroxidation induced by intracortical ferric chloride injection may accountfor the different seizure incidence between young and adult rats
CHARACTERISTICS OF PILI HEMAGLUTININ PROTEIN AND ITS ROLE IN THE PATHOGENESIS OF URINARY TRACT INFECTION WITH UROPATHOGENIC ESCHERICHIA COLI
Authors: Sudhana I Wayan , Suwitra Ketut , - Sumarno
Published: December 2022

Urinary tract infection (UTI) is one of the most common infectious diseasesencountered in the community. The bacteria most frequently implicated as the causes ofUTI are the Gram-negative bacteria, especially Escherechia coli. Early phase of thepathogenesis of the infection constitutes adhesion of bacteria onto the epithelial cells ofurinary tract. SDS-PAGE examination was carried out to investigate molecule ofhemagglutinin protein and hemagglutination (HA) test continued by adhesion andinhibited adhesion tests.The observation of molecular weight of pili E. coli protein molecule byexplorative examination using SDS-PAGE showed on E. coli strip that the mostprominent molecular weights (MW) of the proteins were 61 kDa, 37 kDa, 30 kDa, and 20kDa. Purification by electro-elusion was done to proteins of the MW of 20 kDa, 37 kDaand 61 kDa. The HA test results indicated the 61 kDa, 37 kDa, and 20 kDa werehemagglutinin proteins and one which subsequently applied as sub unit proteins ofUropathogenic E. coli (UPEC) was the 37 kDa. Adhesion test of UPEC bacteria carriedout on urinary bladder epithelial cells of rabbit showed a significant correlation betweenadhesion index and various doses of subunit proteins of UPEC 37 kDa as coat of urinarybladder epithelial cells. Spearman rank correlation test and regression/non regressionanalysis confirmed an exponential decrease of UPEC adhesion to urinary tract epithelialcells as a response to the increasing doses of coating protein. This finding points out thatpili hemagglutinin protein subunit of UPEC 37 kDa was an adhesive molecule. Pilihemagglutinin protein subunit of UPEC 37 kDa is an adhesive molecule, which plays arole in adherence of UPEC to epithelial cells of urinary bladder at the early pathogenesisof urinary tract infection.
Ergonomic Intervention on the Stamping Process of Part Body Component Improved Work Quality and Satisfaction and Time Efficiency at the Stamping Plant Division of PT ADM Jakarta
Authors: Isna Oesman Titin , Manuaba A. , Adiputra N. , Sutjana D.P.
Published: December 2022

Part body component of a car is the finished product component that is mainlyproduced by the big press machine. In the production process of part body component of acar in the stamping plant division, the manual task is performed by two operators to feedingthe material sheet into the big press machine simultaneously. After the knobs were pressedtogether, the big press machine started to stamp the material sheet and the outcoming productis taken by the next operator. This task is performed repeatedly until it is the last one.Manual and comprehensive task that combined with speed in operating the big press machinecause who muscular tension which at the end could cause muscular complaints and prematurefatigue, that could decrease production and productivity.The current study of ergonomic intervention on stamping process of part bodycomponent of car was conducted in order to determine whether ergonomic intervention couldimprove the work quality, satisfaction and time efficiency in the stamping process.The subjects of study were 10 persons that were taken randomly. It was designedexperimentally with treatment by subject design. The method of data collection was byquestionnaire distribution and direct measurement of subjectâ€™s condition, time of stampingprocess and work environment before and after intervention. The Shapiro Wilk test showedthat all of the data had normal distribution and was followed by t-paired for data of workquality, work satisfaction, time efficiency, and the environment data. The significance level isset at 5%.The research result showed that work quality was improved (muscular complaints wasdecreased by 6,65%, fatigue was decreased by 5,47% and work boredom was decreased by5,87%), work satisfaction was improved up to 6,43%, time efficiency of stamping processwas improved up to 10,7%, the production was improved up to 2,59% and productivity wasimproved up to 32,65% as well as companyâ€™s profit was increased up to 3,95%.Hence, the study concludes that ergonomic intervention in the production process ofpart body component of car in the stamping plant division could improve work quality, worksatisfaction and time efficiency.
INHIBITION OF BIFIDOBACTERIUM sp ISOLATED FROM INFANTS FECES TOWARDS ADHESION OF SALMONELLA TYPHI ON BALB/c MICE ENTEROCYTE
Authors: Sukrama I D. M. , Sukardika K. , - Sumarno , Mantik Astawa N.
Published: December 2022

Diarrhea, up to the recent year remains a cause of high morbidity and mortalityworldwide, especially in developing countries including Indonesia. Research concerning ofmanagement, prevention, and medication of the disease have been continually improved. Theaim of this research is searching Bifidobacterium sp isolated from infants feces. ThisBifidobacterium was then applied as an anti-adhesion of Salmonella typhi in the hope to gain acure of diarrhea. This research employed two research designs, namely descriptive explorationand true experimental. Exploration was applied in order to obtain isolation and characterizationof Bifidobacterium isolated from infants feces. Adherence ability of this Bifidobacterium sptowards Salmonella typhi adhesion on mice entherocyte was then carried out by applyingRandomized Posttest-Only Control Group Design. In this research, average Bifidobacterium spadhesion index of 1950 on entherocyte was obtained. In simple word, there are 19.5Bifidobacteria adhere to any single entherocyte cell. This adhesion index value is highercompare to Salmonella typhi adhesion of 1504. Conclusions that can be drawn from this researchare the finding of Bifidobacterium sp isolated from infants feces. This Bifidobacterium sp has anability to inhibit adhesion of Salmonella typhi on BALB/c mice enterocyte. Future work that canbe carried out are further researches concerning whether these bacteria have an ability to inhibitadherence of other pathogen bacteria. More over, searching of cell wall adhesin ofBifidobacterium sp that can be used as a replacement of life probiotic bacteria is also a greatinterest of research to be carried out.
ERGONOMIC INTERVENTION DECREASES THE LOAD OF LEARNING AND INCREASES THE STUDENTS PERFORMANCES
Authors: Pungus Meity , Manuaba Adnyana , Adiputra Nyoman , Tirtayasa Ketut
Published: December 2022

Most studentsâ€™ activities in respect to the implementation of the academic curriculumare done at home. In fact, conventional activity done by the students in boardinghouse ofMinahasa tradition showed unfavorable risks to students based on the load of learning withindicators of musculoskeletal complaints, boredom, and fatigue. Consequently, thestudents were not yet able to attain the intended performance. To overcome the problems,it has made the efforts of ergonomics intervention. In order to test the capability of theergonomics interventions, it had been done a research with the hypothesis, the ergonomicsinterventions on the activity in boardinghouse of Minahasa tradition: normalizes the bodytemperature; decreases the musculoskeletal complaints; decreases the level of boredom;decreases the level of fatigue; increases the accuracy of working; increases the speed ofworking; increases the constancy of working; and increases the results of working of thestudents. This research was done in two periods by utilizing the treatment by subjectdesign. The first and second period was done during four weeks, without and withergonomic intervention. The subjects utilized in the research were as many as 15 persons.The measurements of dependent variables were done before and after doing the activity.The results of the research showed that the ergonomics interventions on the learningactivities in boardinghouse could decrease load of learning significantly (p<0.05): the bodytemperature became normal, the musculoskeletal complaints decreased 44.79%; the levelof boredom decreased 9.79%; the level of fatigue decreased 15.20%; the accuracy ofworking decreased 55.06%; the speed of working decreased 17.32%; the constancy ofworking decreased 30.72%; and the results of working of the students increased 131.44%.It can be concluded that the ergonomics interventions can: decrease the load of learning ofthe students and increase the performance of the students.
ERGONOMIC INTERVENTION IN ORGANIZATION AND WORK STATION INCREASES STUDENTâ€™S WORK PERFORMANCE AND EFFICIENCY OF ELECTRICAL ENERGY CONSUMPTION
Authors: Sudiajeng Lilik , Adiputra N. , Manuaba A. , Sutjana D.P.
Published: December 2022

Woodworking workshop could be hazardous to workersâ€™ health, particularly when itis done improperly. Workers could be exposed to the following risks such as overexertion,repetitive motion, noise, dust, and chemicals that may threaten workersâ€™ health; decreaseswork inefficiency and excessive strains due to inappropriate working condition or posture.This study was conducted to examine the effect of ergonomics intervention in the studentsâ€™work performance and the efficiency of the electrical energy consumption at thewoodworking workshop of Bali State Polytechnic.This is an experimental study with pre and post test control group design. Subjectswere divided into 4 groups of students, each consisting of 10 students, all performing thesame task with different working condition: (1) the original working condition for controlgroup (CG); (2) a new organization for treatment group 1 (TG1); (3) a new work station fortreatment group 2 (TG2); and (4) both new organization and work station for treatmentgroup 3 (TG3). Normally distributed and equal data were analyzed by using the One WayAnova test and followed by the Post Hocâ€“LSD test, and the remaining data were analyzedby using the Kruskal Wallis test and followed by the Mann-Whitney test at the level ofsignificance ? = 0.05.Result showed that studentsâ€™ work performance in TG1 increased in moderately,while those in TG2 and TG3 increased very considerably. Moreover, there was nosignificant increased of the efficiency of electrical energy consumption in TG1 (p > 0.05),but there were significant increased of electrical energy consumption of about 44.74% and107.89% respectively in TG2 and TG3 (p < 0.05). This gain were accompanied by savingcost of the electrical energy consumption of about 38.64% and 58.68% respectively. It alsoappeared that the increased studentsâ€™ work performance and the efficiency of electricalenergy consumption were highest in TG3.In conclussion, it may be stated that comprehensive ergonomic intervention in boththe organization and work station in an effort to apply total ergonomic approach hasproduced the best effect in terms of increased studentsâ€™ work performance and efficiency ofelectrical energy consumption at the woodworking workshop Bali State Polytechnic.
ALDEHYDE DEHIDROGENASE LEVEL AND FATTY ACID ETHYL ESTER AS BIOCHEMICAL MARKERS PERSIST LONGER THAN ETHANOL IN WISTAR RATS AFTER CHRONIC ALCOHOL CONSUMPTION
Authors: Suaniti Ni Made , Sudewa Djelantik A. A. Gde , Suastika Ketut , Mantik Astawa Nyoman
Published: December 2022

Alcohol consumption in human has increased from year to year in Indonesia and more recently, anincreasing number of cases of alcohol intoxication, alcoholic liver disease, and death were observed.The purpose of this experimental study was to examine the significance of two known biochemicalmarkers of alcohol given by mouth in the Wistar rats. The study design used was the â€œTruerandomized experimental post test only control group design". The rats were randomly distributedaccording to the experimental design and were treated daily for six weeks (chronic intake) with 5%and 20% alcohol. This study used 15 rats with 5 rats for treatment group treated with 5% alcohol, 5rats for treatment group treated with 20% alcohol, and 5 rats as control group treated with distilledwater. The biochemical markers were aldehyde dehydrogenase (ALDH) and Fatty Acid Ethyl Esters(FAEE). ALDH and FAEE were two biochemical markers of ethanol which are sensitive and specificfor alcohol consumption. The study was conducted in two phases. Initially, rats were treated orallyeveryday for six weeks with 5% and 20% alcohol, and then the blood level of ethanol, ALDH andFAEE were measured. Blood samples were collected at 6 and 24 hours after the last oral intake ofchronic alcohol administration. Qualitative analysis was carried out to detect the presence of ethanol,ALDH, and FAEE in the treatment groups and quantitative analysis to determine their levels in theblood of Wistar rats. Statistical analysis of ALDH was done by using parametric test and the presenceof FAEE persisting longer than ethanol by non-parametric test. The results showed that ALDHpersisted and increased significantly following chronic consumption of alcohol in the rats. Similarly,FAEEs persisted longer than ethanol after alcohol intake. After six hours, the ALDH level increasedby 108.14% in the rat treated chronically with 5% alcohol and by 85.07% in rat treated with 20%alcohol. After 24 hours, FAEE also persisted longer in the blood than ethanol following treatmentwith alcohol 5%. ALDH levels increased by 83.11% after chronic treatment with 5% alcohol and by112.05% in the rats treated with 20% alcohol. In the blood collected 24 hours after the last treatmentwith 5% alcohol, ALDH increased by 95.11% and by 86.79% in the rats treated with 20% alcohol.FAEE persisted longer than ethanol in the blood following administration of 5 % and 20% alcoholboth at 24 hours following chronic treatment. The longer persisting ALDH and FAEE were new andgood biochemical blood markers for chronic alcohol consumption in the Wistar rats.
CHANGING BODY POSTURE AND WORKING SYSTEM IMPROVES WOKERS PERFORMANCE AND PRODUCT QUALITY
Authors: Surata I Wayan , Manuaba A. , Adiputra N. , Sutjana D.P.
Published: December 2022

Seaweed is one of the nationâ€™s potential marine commodities that maycontribute to the national revenue and a new source of income for the localcommunity, as the cultivation of seaweed is much easier and cheaper than paddycrop, because neither pesticides nor fertilizer would be required. Other advantages ofcultivating seaweed are the fact that it can be carried out throughout the year, and itsrelatively shorter duration to reach the harvesting time. Local practice of drying theseaweed is by spreading it over a plastic sheet on the ground and exposing it to thesun. Unfortunately, such traditional practice of drying seaweed has led to increasedrisk of contamination of seaweed by dust and sand particles and other impurities.The quality of seaweed is influenced by the type of seedlings, harvesting age, andthe crucial process of drying. The local traditional working posture during drying issquatting and stooping to flatten and spread evenly the seaweed. Our preliminarystudy has shown that the farmers have had considerable musculoskeletal complaintsand fatigue with the seaweed quality being still relatively low.This study was conducted to examine the effects of changing the workersâ€™working posture and limiting lift weight at 23 kg and introduced a 5-minute breakafter an-hour work with the aim to improve both workersâ€™ performance and seaweedquality and productivity. This is an experimental study using a two-period crossoverdesign on 20 farmers of seaweed cultivation in the Ped Village of Nusa Penida.The results of our study showed that redesigning and changing the workersâ€™working posture and working system improved the performance of seaweed farmersas revealed by the decrease of musculoskeletal complaints to 56.15% (p < 0.05); bydecrease in general fatigue to 50.84% (p < 0.05); by the increase in productivity to37.93% (p < 0.05), and by the increase in income to 41.62% (p < 0.05). The qualityof the dried seaweed was also improved as could be seen from the decrease in watercontent to 29.24% (p < 0.05) and decrease in impurities to 33.33% (p < 0.05).From our data it could be inferred that changing the workersâ€™ working bodyposture and working system significantly improved both their performance and thequality of their product.
CENTELLA ASIATICA EXTRACT INCREASED ON THE LEVEL OF INTERLEUKIN 6 (IL-6) IN MICE
Authors: Kerta Besung I Nengah , Mantik Astawa Nyoman , Suatha I Ketut , - Hartaningsih
Published: December 2022

Salmonellosis is still problem in many developing countries including Indonesia. Themain problem in controlling and handling the disease is that only few antibiotics are availableto cure the disease. In addition, the prolonged use of such antibiotics often lead to bacterialresistant against the antibiotics. A herbal drugs such as Centella asiatica (in Indonesia isknown as pegagan) contains triterphenoid saphonins which acts as immunostimulant capableof enhancing the phagocytic activity of macrophages. However, no study has been conductedto investigate the use pegagan in activating macrophage of mice infected with Salmonellatyphi. A study was therefore conducted to find out the ability of Cantella asiatica inenhancing on the level interluekine (IL)-6 following challenge with Salmonella typhi. It istherefore expected that herbal drug such as Cantella asiatica can be used as an alternativemedicine to prevent and cure salmonellosis in both animals and human.Experimental laboratory studies were conducted using Completely FactorialRandomized Design. Mice were divided into 4 groups and they were treated respectively withdestilated water (negative control), 125, 250, and 500 mg/kg bw of Centella asiatica extract.The treatment was conducted daily for 2 weeks and the mice were then inoculated with 105cells of S. typhi. The level of IL-6 response were examined 24 hours, 2 weeks, and 4 weeksafter inoculation with S. typhi.The result showed that treatment of mice with Centella asiatica extract significantly(p<0,05) enhaced IL-6 level of Balb/c mice following inoculation with S. typhi. The highes tIL-6 level were observed in mice treated Centella asiatica extract at the dose of 500 mg/kgBW (385,9257Â±125,4314 pg/ml serum). And the highest IL-6 level were observed at 2 weeksfollowing inoculation with S. typhi (533.4262 Â± 81.7184 pg / ml).A further study is recomended to examine the celluler immune response and moredetailed study on the humoral immune response of animals or human before this herbal isused as alternatif medicine to prevent and cure typhoid fever. It is also important to study thebest preparation, the half life, and the side effect of Centella asiatica in human and animals.
STEROID FOR MANAGEMENT OF PSEUDO-OTHEMATOMA
Authors: Sudana Wayan , Adiputra Nyoman , Wiyadi H.M.S.
Published: December 2022

Has conducted research on the use of steroids triamsinolon asetonid as pseudo-othematomamedical therapy. The purpose of this study was to determine the effectiveness triamsinolonasetonid. Prevention of the formation of serum in the lesion, so that pseudo-othematomacan be cured without surgery. Surgery and preventing of the formation collagen tissue inthe lesion, so that the pseudo-othematoma can be cured without deformity. It is expectedthe establishment of a pseudo-othematoma practical for health personnel, effective,efficient, safe and comfortable for the patient. This study design is the design of arandomized controled pretest-postest design. In the test group were treated withtriamsinolon asetonid intracutaneus injected in lesion area with the number according tothe percentage of the amount of teraphy effusion before. Then press verban installed. In thecontrol group, only pressing verban installed. From the research results obtained, whishafter the third treatment using the triamsinolon asetonid, pseudo-othematoma diseaselooked very meaningful. Due to its unique histological structure of the ear, then themanipulation of the ear is moe advisable to be careful, to prevent new lession with variousimpacts.
APPLYING BASIC ERGONOMIC PRINCIPLES IMPROVES THE QUALITY OF TEACHING-LEARNING ENVIRONMENT IN THE JUNIOR SECONDARY SCHOOL
Authors: Ardana I G.N. , Manuaba A. , Adiputra N. , Sutajaya M.
Published: December 2022

This study was conducted at the relatively young public junior secondary school at the district of Abiansemal and Administrative Region of Badung (SMPN-3) now aspiring to obtain national accreditation. In this context, the need was felt for improving the teaching-learning environment particularly in the schoolâ€™s classrooms which might be conducive to maximizing the educational input and student performance. We studied the impacts of applying basic ergonomic principles to the design of some physical facilities (including redesign of the tables and chairs) in the classroom aiming to remediate present perceived deficiencies. Our samples were selected by using the multistage simple random sampling technique; and a total of 81 subjects consisting of 43 male and 38 female, volunteer students were recruited. The statistical methods used include descriptive statistics, analysis of normality and comparability. Our findings implied that interior ergonomic design intervention has led to the following outcomes: enhancing the student performance by decreasing eye strain by 44.76%; musculoskeletal complaints by 50.98%; and boredom by 14.17%. The beneficial outcomes of our intervention appeared to be obvious.
SEAWEED EXTRACTS IMPROVE LIPID PROFILE OF WISTAR RAT
Authors: Marhaeni Julyasih K. Sri , Suata K. , Wirawan I.G.P , Mantik Astawa I. N.
Published: December 2022

Hypercholesterolemia or hyperlipidemia has been established as an important risk factor of cardiovascular disease. Patients with hypercholesterolemia usually require a prolonged treatment; and the newer and more potent generation of antilipid agents are costly.In Bali there are several types of seaweed that are generally consumed by the local people and known by the local names of Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.). Preliminary studies on the effect of Bulung Boni and Bulung Sangu extracts appeared to improve lipid profile, but the available data are still very limited both in extent and depth, and further investigations were considered relevant and needed.This experimental study used completely blind randomized design, using a total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol content. The six sample groups were respectively designated as negative control group, positive control group, and four treated sample groups, respectively fed orally with a dose of 20 mg and 60 mg extracts of Bulung Boni per 100g of body weight per day, and 20 mg and 60 mg extracts of Bulung Sangu per 100g body weight per day. Each treatment was repeated four times.Our study showed that rats fed with high-cholesterol diet and treated with oral Bulung Boni or Bulung Sangu extract at a dose of 20 mg and 60 mg/100 g bw/ day were associated with statistically significantly increased plasma HDL levels (p < 0.05), and statistically significant decreased plasma LDL and total cholesterol levels (p < 0.05) as compared with those of rats fed with high cholesterol diet without treatment with Bulung Boni or Bulung Sangu extracts.From our data it could be implied that Bulung Boni and Bulung Sangu extracts improve lipid profile in the Wiatar rat by significantly increasing plasma HDL level, and lowering LDL and total cholesterol levels.
TOPICAL APPLICATION OF ETHANOL EXTRACT OF STARFRUIT LEAVES (AVERRHOA BILIMBI LINN) INCREASES FIBROBLASTS IN GINGIVAL WOUNDS HEALING OF WHITE MALE RATS
Authors: Hartini IGAA
Published: December 2022

ABSTRACT Injuries to the gingiva are often encountered because of abnormalities in the oral cavity. Gingival wound healing is more complex because it lies in an open area, often contaminated and exposed to various types of bacteria in the oral cavity. Gingiva wound healing is determined by increase of fibroblasts. Plants widely used by humans as medicine is star fruit (Averrhoa Bilimbi Linn). This study aims to determine an increase of gingival fibroblasts on wound healing of male white rats given various concentrations of starfruit leaves extract topically. The study was conducted with a pretest-posttest control group design, consisted of four groups: the control group with distilled water and the groups treated with ethanol extract of starfruit leaves with a concentration of 10%, 20% and 40%. The result based on comparison test between the groups with One Way Anova, showed that the average amount of fibroblast in four groups after receiving the treatment was significantly different (p<0.01). The result of the test showed an increase in the number of fibroblasts in groups concentration of 10% and 20%, there was a decline observed in group concentration of 40%. It was concluded that application of ethanol extract of starfruit leaves with concentration of 10% increase the highest number of fibroblasts in the rat gingival wound healing process.
VARIES SHUTTLE RUN EXERCISE METHODS INCREASE AGILITY OF STUDENTS OF SMK NEGERI 2 TONDANO KABUPATEN MINAHASA
Authors: Sarapung M , Pangkahila J. A. , Adiputra N. , Adiatmika IPG
Published: December 2022

Achievement of Indonesian athletes at international level are only resting on badminton, archery, and weight lifting. Meanwhile, other sports do not exhibit encouraging achievement. Agility is one of the important factor on sports including group and individual sports. This is an experimental research with randomized pretest posttest conrol group design. Sample are 75 students recruited from SMK Negeri 2 Tondano Kabupaten Minahasa. Sample was grouped into 5 groups, each group composed of 15 students. The five groups observed were group 1 with treatment of 4 x 30 m shuttle run, group 2 treated with 8 x 15 m shuttle run, group 3 with 12 x 10 m shuttle run, group 4 within 16 x 7.5 m shuttle run, and the last is control group. Exercise was carried out for 6 weeks with a frequent of 3 time per week. Agility pre and posttest data were analysed using anova oneway followed by LSD Post Hoct Test at 5% significant level. This study obtained that there were a significant different among the five groups observed. Shuttle run exercise method with dose of 16x7.5 m increase the highest agility.Â