bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of Conditions for Feather Waste Biodegradation by Geophilic Trichophyton ajelloi Fungal Strains towards Further Agricultural Use
Authors: Możejko Michał , Bohacz Justyna
Published: September 2022

The aim of the study was to optimize culture conditions and medium composition to accelerate the biodegradation of chicken feather waste by keratinolytic soil strains of Trichophyton ajelloi, which are poorly known in this respect, as well as to propose hitherto unconsidered culture conditions for these fungi in order to obtain a biopreparation with a high fertilization value. Different pH of the medium, incubation temperatures, amounts of chicken feathers, additional carbon sources, and culture methods were tested. The process of optimizing keratin biodegradation was evaluated in terms of measuring the activity of keratinase, protease, disulfide reductase, concentration of released soluble proteins and peptides, total pool of amino acids, ammonium and sulfate ions, changes in medium pH, and feather weight loss. It was found that the studied fungal strains were capable of decomposing and mineralizing keratin from feather waste. Regarding the fertilizer value of the obtained hydrolysates, it was shown that the release of sulfate and ammonium ions was highest in a stationary culture containing 2% feathers with an initial pH of 4.5 and a temperature of 28 °C. Days 14–21 of the culture were indicated as the optimal culture time for these fungi to obtain biopreparations of high fertilizing value.
Identifying the Spatio-Temporal Characteristics of Crime in Liangshan Prefecture, China
Authors: Cheng Wuxue , Rao Yajun , Tang Yixin , Yang Jiajia , Chen Yuxin , Peng Li , Hao Jiangcheng
Published: September 2022

Crime prevention and governance play critical roles in public security management. Liangshan Yi Autonomous Prefecture in Sichuan Province has a high crime rate, and spatio-temporal analysis of crime in this region could assist with public security management. Therefore, Liangshan Prefecture was selected as the research object in this study. The spatial crime data were obtained from China Judgments Online, and property crime, violent crime, and special crime (i.e., pornography, gambling, drugs, and guns) were analyzed. The findings were as follows. In terms of time characteristics (month, day, and hour), property crime tended to occur in autumn and winter, in the early month, on Wednesdays and Fridays, and at early morning. Violent crime tended to occur in winter and spring, on Mondays and Thursdays, and at night. Special crime occurred in spring and autumn, on Tuesdays, and in the daytime. In terms of spatial features, the central region of Liangshan Prefecture was the focal area for crime. There were obvious low-aggregation areas in the western region for special crime. The eastern region exhibited a high incidence of various crimes. Regarding the spatio-temporal evolution characteristics from 2013 to 2019, there were some obvious hotspots of violent and property crime in downtown and surrounding townships of Xichang City, which is the capital of Liangshan Prefecture. During the study period, the incidence of special crime has an obvious downward trend which shows that there are more new cold spots.
Measuring Obesogenicity and Assessing Its Impact on Child Obesity: A Cross-Sectional Ecological Study for England Neighbourhoods
Authors: Congdon Peter
Published: September 2022

Both major influences on changing obesity levels (diet and physical activity) may be mediated by the environment, with environments that promote higher weight being denoted obesogenic. However, while many conceptual descriptions and definitions of obesogenic environments are available, relatively few attempts have been made to quantify obesogenic environments (obesogenicity). The current study is an ecological study (using area units as observations) which has as its main objective to propose a methodology for obtaining a numeric index of obesogenic neighbourhoods, and assess this methodology in an application to a major national dataset. One challenge in such a task is that obesogenicity is a latent aspect, proxied by observed environment features, such as poor access to healthy food and recreation, as well as socio-demographic neighbourhood characteristics. Another is that obesogenicity is potentially spatially clustered, and this feature should be included in the methodology. Two alternative forms of measurement model (i.e., models representing a latent quantity using observed indicators) are considered in developing the obesogenic environment index, and under both approaches we find that both food and activity indicators are pertinent to measuring obesogenic environments (though with varying relevance), and that obesogenic environments are spatially clustered. We then consider the role of the obesogenic environment index in explaining obesity and overweight rates for children at ages 10–11 in English neighbourhoods, along with area deprivation, population ethnicity, crime levels, and a measure of urban–rural status. We find the index of obesogenic environments to have a significant effect in elevating rates of child obesity and overweight. As a major conclusion, we establish that obesogenic environments can be measured using appropriate methods, and that they play a part in explaining variations in child weight indicators; in short, area context is relevant.
Designing Multifunctional Urban Green Spaces: An Inclusive Public Health Framework
Authors: Lafrenz Andrew J.
Published: September 2022

Evidence of the wide range of health benefits associated with the use of urban green space (UGS) continues to grow. Despite this evidence, many UGS designs do not adopt a community-inclusive approach that utilizes evidence-based public health strategies to maximize potential health benefits. This research focused on testing a multidisciplinary, community-involved public health framework to drive the UGS design process. The aim of this study was to use community feedback and evidence-based public health practices to promote physical health, psychological wellbeing, and social cohesion by creating a multifunctional UGS that enhances nature therapy, natural play, and sports and recreation. Community health assessment data (236 survey responses), community forum and survey feedback (157 survey responses), local urban green space inventory assessment, and environmental assessment and impact data were analyzed to develop a design plan that maximize the greatest potential health benefits for the greatest proportion of the population. Community health data indicated a strong relationship between the availability of places to be physically active in the community and higher ratings of mental (aOR = 1.80) and physical (aOR = 1.49) health. The creation and utilization of the proposed community-inclusive and public health-focused framework resulted in a UGS design that prioritized the needs of the community and provided evidence-informed strategies to improve the health of local residents. This paper provides unique insight into the application of a framework that promotes a more health-focused and functional approach to UGS design.
Constructing the Ecological Security Pattern of Nujiang Prefecture Based on the Framework of “Importance–Sensitivity–Connectivity”
Authors: Li Yimin , Zhao Juanzhen , Yuan Jing , Ji Peikun , Deng Xuanlun , Yang Yiming
Published: September 2022

Constructing an ecological security pattern is vital to guaranteeing regional ecological security. The terrain and geomorphology of the alpine valley are complex and sensitive, meaning it is difficult to construct ecological security patterns. Therefore, the study takes Nujiang Prefecture as the study area and builds an “Importance–Sensitivity–Connectivity” (Importance of ecosystem service, eco-environmental sensitivity, and landscape connectivity) framework to carry on the comprehensive evaluation of the ecological security and identification of ecological sources. Furthermore, we constructed an ecological resistance surface using land-use type. Using the minimum cumulative resistance (MCR) model, the study identifies the ecological corridors and nodes to build ecological security patterns to optimize the ecological spatial structure of Nujiang Prefecture. The results showed that (1) the importance of ecosystem services was higher in the west and lower in the east. The high-sensitive areas of the ecological environment were distributed discontinuously along the banks of the Nujiang and the Lantsang River, and the areas with high landscape connectivity were distributed in patches in the Gaoligong Mountain Nature Reserve and the Biluo Snow Mountain. (2) The overall ecological security was in a good state, and the ecologically insecure areas were primarily distributed in Lanping County and the southeast region of Lushui City. (3) The primary ecological source area was identified to be 3281.35 km2 and the secondary ecological source area to be 4224.64 km2. (4) In total, 26 primary ecological corridors, 39 secondary ecological corridors, and 82 ecological nodes were identified.
Male-Type Depression Mediates the Relationship between Avoidant Coping and Suicidal Ideation in Men
Authors: O’Gorman Kieran M. , Wilson Michael J. , Seidler Zac E. , English Derek , Zajac Ian T. , Fisher Krista S. , Rice Simon M.
Published: September 2022

Despite known links between men’s avoidant coping behaviours (e.g., distraction, denial, substance use) and suicide risk, little research has explored the mechanisms underpinning this relationship. This study sought to examine whether male-type depression symptoms (e.g., anger, aggression, emotion suppression), assessed by the Male Depression Risk Scale, mediate the association between avoidant coping and suicide/self-harm ideation in men. Data were drawn from an online survey of a community sample of 606 Australian men (M age = 50.11 years; SD = 15.00), conducted during the COVID-19 pandemic. Mediation analyses were applied to examine the effect of male-type depression on the association between avoidant coping and suicidal/self-harm ideation, controlling for age, resilience and the experience of two psychosocial stressors during the COVID-19 pandemic (financial stress and government restrictions). Avoidant coping was associated with suicidal/self-harm ideation, r = 0.45, p < 0.001. Results supported a mediating role of male-type depression symptoms in this relationship, R2= 0.29, PM = 0.36, p < 0.001, underscoring the importance of screening for male-type depression symptoms to better identify men at risk of suicidal/self-harm ideation. Results also suggest a need to support men to develop effective coping strategies, particularly in the context of common psychosocial stressors experienced during the COVID-19 pandemic and beyond.
Derivation of Water Quality Criteria for Carbamazepine and Ecological Risk Assessment in the Nansi Lake Basin
Authors: Wu Jiangyue , Shi Dianlong , Wang Sai , Yang Xi , Zhang Hui , Zhang Ting , Zheng Lei , Zhang Yizhang
Published: September 2022

Carbamazepine, as one of several pharmaceutical and personal care products, has gained much attention in recent years because of its continuous discharge in natural waters and toxicity to aquatic ecosystems. However, it is difficult to evaluate and manage carbamazepine pollution because of the lack of a rational and scientific Water Quality Criteria (WQC) of carbamazepine. In this study, the carbamazepine toxicity data of thirty-five aquatic species from eight taxonomic groups were selected, and the species sensitivity distribution (SSD) method was applied to derive the WQC for carbamazepine based on the Log-logistic model, which was 18.4 ng/L. Meanwhile, the occurrence and distribution of carbamazepine in the Nansi Lake basin was studied. Results showed that concentrations of carbamazepine in 29 sampling sites were in the range of 3.3 to 128.2 ng/L, with the mean of 17.3 ng/L. In general, the levels of carbamazepine in tributaries were higher than those in the lakes. In addition, qualitative and quantitative ecological risk assessment methods were applied to assess the adverse effect of carbamazepine on aquatic systems. The hazard quotient (HQ) method showed that there were 24 and 5 sampling sites, in which risk levels were low and moderate, respectively. The joint probability curve (JPC) method indicated that ecological risks might exist in 1.4% and 1.0% of surface water, while a 5% threshold and 1% threshold were set up to protect aquatic species, respectively. Generally, carbamazepine posed a low risk to the aquatic organisms in the Nansi Lake basin.
Novel Approaches for Inhibiting the Indoor Allergen Der f 2 Excreted from House Dust Mites by Todomatsu Oil Produced from Woodland Residues
Authors: Lin Yichun , Enyoh Christian Ebere , Wang Qingyue , Lu Senlin , Zhang Wei , Xiao Kai , Zhou Shumin , Kaneko Toshihiko , Seguchi Akifumi , Wang Weiqian , Guo Yue
Published: September 2022

House dust mite (HDM) is a globally ubiquitous domestic cause of allergic diseases. There is a pressing demand to discover efficient, harmless, and eco-friendly natural extracts to inhibit HDM allergens that are more likely to trigger allergies and challenging to be prevented entirely. This study, therefore, is aimed at assessing the inhibition of the allergenicity of major HDM allergen Der f 2 by todomatsu oil extracted from residues of Abies Sachalinensis. The inhibition was investigated experimentally (using enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)) and in silico using molecular docking. The results showed that todomatsu oil inhibits the allergenicity of Der f 2 by reducing its amount instead of the IgG binding capacity of a single protein. Moreover, the compounds in todomatsu oil bind to Der f 2 via alkyl hydrophobic interactions. Notably, most compounds interact with the hydrophobic amino acids of Der f 2, and seven substances interact with CYS27. Contrarily, the principal compounds fail to attach to the amino acids forming the IgG epitope in Der f 2. Interestingly, chemical components with the lowest relative percentages in todomatsu oil show high-affinity values on Der f 2, especially β-maaliene (−8.0 kcal/mol). In conclusion, todomatsu oil has been proven in vitro as a potential effective public health strategy to inhibit the allergenicity of Der f 2.
Assessing and Responding to Suicide Risk in Children and Young People: Understanding Views and Experiences of Helpline Staff
Authors: Williams A. Jess , Kloess Juliane A. , Gill Chloe , Michail Maria
Published: September 2022

Introduction: Suicide is a key issue impacting children and young people. Helplines offer unique benefits, such as anonymity, varied communication avenues and low cost, which help to promote help-seeking behaviour. The aim of this study was to explore the views and experiences of helpline organisations of identifying, assessing, and managing suicide risk among children and young people. Methods: Thirteen professionals from three UK-based helplines and online counselling services took part in semi-structured interviews between November 2020 and January 2021 via Zoom. Interviews were transcribed verbatim and analysed using Thematic Analysis. Results: Three superordinate themes were identified: (i) Starting conversations about suicide; (ii) Identifying and responding to “imminent” suicide risk; and (iii) Responses to suicide risk in relation to safeguarding. Limitations: Recruitment was limited by COVID-19 due to the demands needed from helplines at this time. Conclusions: Our findings highlight not only the different types and range of services helpline organisations offer to young people who might be at risk of suicide, but most importantly the distinct role they have in young people’s help-seeking pathway.
When the Normative Is Formative: Parents’ Perceptions of the Impacts of Inclusive Sports Programs
Authors: Rodriquez Jason , Lanser Anika , Jacobs Holly E. , Smith Ashlyn , Ganguly Sharbari
Published: September 2022

This qualitative study examines the perspectives of parents of youth with intellectual and developmental disability (IDD) who participated in Special Olympics Unified Sports®, a program in which high school students with and without IDD compete on the same team. Based on semi-structured interviews with parents (n = 23) as part of a larger evaluation of Special Olympics Unified Champion Schools in three states in the United States, thematic analysis shows that parents perceived improvements in their child’s social and emotional skills, including demeanor and attitude, an enhanced sense of belonging at school, the emergence of new friendships and social opportunities, and rewarding interactions that flowed from the opportunity to engage in normative activities. Implications for schools and families are discussed in terms of the importance of providing and facilitating meaningful opportunities for inclusive extracurricular activities such as sports for youth with IDD.
Activated Porous Carbon Fiber: New Adsorbent for Sampling and Analysis by Thermal Desorption of Siloxanes in Biogas and Biomethane
Authors: Paris Enrico , Avino Pasquale , Guerriero Ettore , Vincenti Beatrice , Palma Adriano , Carnevale Monica , Benedetti Paolo , Torre Marco , Gallucci Francesco
Published: September 2022

The growing global energy demand requires the continuous development and optimization of the production of alternative energy sources. According to the circular economy approach, waste conversion into biogas and biomethane represent an interesting energy source. The input into the distribution network and energy conversion systems of biomethane requires quality monitoring and the use of cleaning up systems. Therefore, there is a need to constantly invest in the development of sampling and analysis systems that save time, costs, and materials. The purpose of this study was to use activated porous carbon fiber (APCF), an extremely versatile material for sampling and analysis by thermal desorption, to show the advantages it has over the adsorbents traditionally used for siloxane monitoring. Siloxanes are among the contaminating compounds that are mainly present in biogas and biomethane, and if not removed sufficiently, they endanger the quality and use of the gas. These are highly harmful compounds since during combustion, they produce quartz particles that are abrasive to the surfaces of the materials involved in the energy production process. In addition, siloxanes directly hinder the energy properties of biomethane during combustion, due to their radical scavenger properties. In this work, the efficiency of APCF tube was evaluated by comparing it with common multilayer tube thought sampling and analyzing siloxanes in lab scale and in real scale (biogas plant). Thermal desorption analysis coupled with GC-MS for the determination of siloxanes showed that the use of APCF allows to obtain better performance. This allows to deduce that APCF is an innovative material for the establishment of a better sampling and analysis method than the current ones, enabling better results to be achieved in the process of monitoring fuel quality in biomethane production and storage facilities.
Pain Assessment with the BPS and CCPOT Behavioral Pain Scales in Mechanically Ventilated Patients Requiring Analgesia and Sedation
Authors: Wojnar-Gruszka Katarzyna , Sega Aurelia , Płaszewska-Żywko Lucyna , Wojtan Stanisław , Potocka Marcelina , Kózka Maria
Published: September 2022

Background: Intensive Care Unit (ICU) patients often experience pain, especially during diagnostic, nursing, and therapeutic interventions. Pain assessment using the Behavioral Pain Scale (BPS) and Critical Care Pain Observation Tool (CCPOT) are recommended, but they are difficult to do in patients undergoing deep sedation. This study analyzed the usefulness of the BPS and CCPOT scales in assessing pain among patients with varying degrees of sedation. Methods: In 81 mechanically ventilated and sedated ICU patients, 1005 measurements were performed using the BPS and CCPOT scales. The study was conducted by 3 trained observers 3 times a day (each measurement at rest, during painful nursing interventions, and after the intervention). The Richmond Agitation-Sedation Scale (RASS), the Simplified Acute Physiology Score (SAPS II), and the Acute Physiology and Chronic Health Evaluation (APACHE II) were also analyzed from medical records as well as information on the length of hospitalization and treatment. Results: It was shown that signs of pain increased significantly (p < 0.001) during interventions in patients on both scales (BPS and CCPOT), and then returned to values close to the resting period. RASS results correlated significantly (p < 0.05) and positively with the results of the BPS and CCPOT. A strong correlation was found between the results of both scales at each stage of the study (R = 0.622–0.907). Conclusions: Nursing procedures are a source of pain in analgosedated patients. The BPS and CCPOT scales are useful tools for assessing the occurrence of pain in mechanically ventilated patients, including those in deep sedation.
Cotton (Gossypium hirsutum) VIRMA as an N6-Methyladenosine RNA Methylation Regulator Participates in Controlling Chloroplast-Dependent and Independent Leaf Development
Authors: Huang Xiaoyu , Abuduwaili Nigara , Wang Xinting , Tao Miao , Wang Xiaoqian , Huang Gengqing
Published: September 2022

N6-methyladenosine (m6A) is one of the most abundant internal modifications of mRNA, which plays important roles in gene expression regulation, and plant growth and development. Vir-like m6A methyltransferase associated (VIRMA) serves as a scaffold for bridging the catalytic core components of the m6A methyltransferase complex. The role of VIRMA in regulating leaf development and its related mechanisms have not been reported. Here, we identified and characterized two upland cotton (Gossypium hirsutum) VIRMA genes, named as GhVIR-A and GhVIR-D, which share 98.5% identity with each other. GhVIR-A and GhVIR-D were ubiquitously expressed in different tissues and relatively higher expressed in leaves and main stem apexes (MSA). Knocking down the expression of GhVIR genes by the virus-induced gene silencing (VIGS) system influences leaf cell size, cell shape, and total cell numbers, thereby determining cotton leaf morphogenesis. The dot-blot assay and colorimetric experiment showed the ratio of m6A to A in mRNA is lower in leaves of GhVIR-VIGS plants compared with control plants. Messenger RNA (mRNA) high-throughput sequencing (RNA-seq) and a qRT-PCR experiment showed that GhVIRs regulate leaf development through influencing expression of some transcription factor genes, tubulin genes, and chloroplast genes including photosystem, carbon fixation, and ribosome assembly. Chloroplast structure, chlorophyll content, and photosynthetic efficiency were changed and unsuitable for leaf growth and development in GhVIR-VIGS plants compared with control plants. Taken together, our results demonstrate GhVIRs function in cotton leaf development by chloroplast dependent and independent pathways.
Ligand-Specific Nano-Contrast Agents Promote Enhanced Breast Cancer CT Detection at 0.5 mg Au
Authors: Ramesh Kalyan , Truong Alice , Wang Yuzhen , Rusckowski Mary , Gkikas Manos
Published: September 2022

For many cancer types, being undetectable from early symptoms or blood tests, or often detected at late stages, medical imaging emerges as the most efficient tool for cancer screening. MRI, ultrasound, X-rays (mammography), and X-ray CT (CT) are currently used in hospitals with variable costs. Diagnostic materials that can detect breast tumors through molecular recognition and amplify the signal at the targeting site in combination with state-of-the-art CT techniques, such as dual-energy CT, could lead to a more precise detection and assist significantly in image-guided intervention. Herein, we have developed a ligand-specific X-ray contrast agent that recognizes α5β1 integrins overexpressed in MDA-MB-231 breast cancer cells for detection of triple (−) cancer, which proliferates very aggressively. In vitro studies show binding and internalization of our nanoprobes within those cells, towards uncoated nanoparticles (NPs) and saline. In vivo studies show high retention of ~3 nm ligand-PEG-S-AuNPs in breast tumors in mice (up to 21 days) and pronounced CT detection, with statistical significance from saline and iohexol, though only 0.5 mg of metal were utilized. In addition, accumulation of ligand-specific NPs is shown in tumors with minimal presence in other organs, relative to controls. The prolonged, low-metal, NP-enhanced spectral-CT detection of triple (−) breast cancer could lead to breakthrough advances in X-ray cancer diagnostics, nanotechnology, and medicine.
Cybersecurity Behavior among Government Employees: The Role of Protection Motivation Theory and Responsibility in Mitigating Cyberattacks
Authors: Sulaiman Noor Suhani , Fauzi Muhammad Ashraf , Hussain Suhaidah , Wider Walton
Published: September 2022

This study examines the factors influencing government employees’ cybersecurity behavior in Malaysia. The country is considered the most vulnerable in Southeast Asia. Applying the protection motivation theory, this study addresses the gap by investigating how government employees behave toward corresponding cyberrisks and threats. Using partial least-squares structural equation modeling (PLS-SEM), 446 respondents participated and were analyzed. The findings suggest that highly motivated employees with high severity, vulnerability, response efficacy, and self-efficacy exercise cybersecurity. Incorporating the users’ perceptions of vulnerability and severity facilitates behavioral change and increases the understanding of cybersecurity behavior’s role in addressing cybersecurity threats—particularly the impact of the threat response in predicting the cybersecurity behavior of government employees. The implications include providing robust information security protection to the government information systems.
Parasitism by the Tachinid Parasitoid Exorista japonica Leads to Suppression of Basal Metabolism and Activation of Immune Response in the Host Bombyx mori
Authors: Dai Minli , Yang Jin , Liu Xinyi , Gu Haoyi , Li Fanchi , Li Bing , Wei Jing
Published: September 2022

The dipteran tachinid parasitoids are important biocontrol agents, and they must survive the harsh environment and rely on the resources of the host insect to complete their larval stage. We have previously demonstrated that the parasitism by the tachinid parasitoid Exoristajaponica, a pest of the silkworm, causes pupation defects in Bombyx mori. However, the underlying mechanism is not fully understood. Here, we performed transcriptome analysis of the fat body of B. mori parasitized by E. japonica. We identified 1361 differentially expressed genes, with 394 genes up-regulated and 967 genes down-regulated. The up-regulated genes were mainly associated with immune response, endocrine system and signal transduction, whereas the genes related to basal metabolism, including energy metabolism, transport and catabolism, lipid metabolism, amino acid metabolism and carbohydrate metabolism were down-regulated, indicating that the host appeared to be in poor nutritional status but active in immune response. Moreover, by time-course gene expression analysis we found that genes related to amino acid synthesis, protein degradation and lipid metabolism in B. mori at later parasitization stages were inhibited. Antimicrobial peptides including Cecropin A, Gloverin and Moricin, and an immulectin, CTL11, were induced. These results indicate that the tachinid parasitoid perturbs the basal metabolism and induces the energetically costly immunity of the host, and thus leading to incomplete larval–pupal ecdysis of the host. This study provided insights into how tachinid parasitoids modify host basal metabolism and immune response for the benefit of developing parasitoid larvae.
The Influence of Host Aphids on the Performance of Aphelinus asychis
Authors: Li Zhen-Xiang , Ji Meng-Qi , Zhang Chi , Yang Yi-Bing , Chen Zhen-Zhen , Zhao Hai-Peng , Xu Yong-Yu , Kang Zhi-Wei
Published: September 2022

The aphid parasitoid Aphelinus asychis Walker is an important biological control agent against many aphid species. In this study, we examined whether the rearing host aphid species (the pea aphid, Acyrthosiphon pisum and the grain aphid, Sitobion avenae) affect the performance of A. asychis. We found that A. pisum-reared A. asychis showed a significantly larger body size (body length and hind tibia length) and shorter developmental time than S. avenae-reared A. asychis. There was no difference in the sex ratio between them. The longevity of A. pisum-reared A. asychis was also significantly longer than that of S. aveane-reared A. asychis. Furthermore, A. pisum-reared A. asychis presented stronger parasitic capacity and starvation resistance than S. aveane-reared A. asychi. In addition, host aphid alteration experiments showed that A. asychis only takes two generations to adapt to its new host. Taken together, these results revealed that A. pisum is a better alternative host aphid for mass-rearing and releasing of A. asychis. The body size plasticity of A. asychis is also discussed.
Sex Differences in Frequency of Instrumental Activities of Daily Living after Cardiac Rehabilitation and Its Impact on Outcomes in Patients with Heart Failure
Authors: Nozaki Kohei , Hamazaki Nobuaki , Kamiya Kentaro , Kariya Hidenori , Uchida Shota , Noda Takumi , Ueno Kensuke , Maekawa Emi , Matsunaga Atsuhiko , Yamaoka-Tojo Minako , Ako Junya
Published: September 2022

Although instrumental activities of daily living (IADL) are included in the outcomes of cardiac rehabilitation (CR), the relationship between IADL frequency at the end of CR and outcomes between the sexes remains unclear. We aimed to investigate the differences in frequency of IADL between the sexes and its impact on the outcomes. We retrospectively investigated 490 consecutive patients who were admitted for heart failure (HF) and participated in CR post-discharge. IADL frequency was assessed using the questionnaire-based Frenchay Activities Index (FAI). The primary endpoint was all-cause death, and the secondary endpoint was a composite of all-cause death and readmission due to HF. The cut-off values of the FAI for all-cause death in the overall cohort, females, and males were 23, 22, and 23 points, respectively. After adjusting for several factors, IADL assessed using the FAI was independently associated with all-cause mortality (hazard ratio [HR]: 0.961, 95% confidence interval [CI]: 0.937–0.986) and combined events (HR: 0.968, 95% CI: 0.952–0.985), respectively. Additionally, there was no interaction between sex and all-cause mortality. In conclusion, higher IADL frequency after CR was associated with favourable outcomes in patients with HF.
Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
Authors: Li Siting , Meng Jingjing , Lv Yanze , Wang Qian , Tian Xinping , Li Mengtao , Zeng Xiaofeng , Hu Chaojun , Zheng Yuehong
Published: September 2022

Background: B cells and autoantibodies play an important role in the pathogenesis of abdominal aortic aneurysm (AAA). IgG glycosylations are highly valued as potential disease biomarkers and therapeutic targets. Methods: Lectin microarray was applied to analyze the expression profile of serum IgG glycosylation in 75 patients with AAA, 68 autoimmune disease controls, and 100 healthy controls. Lectin blots were performed to validate the differences. The clinical relevance of lectins binding from the microarray results was explored in AAA patients. Results: Significantly lower binding level of SBA (preferred GalNAc) was observed for the AAA group compared with DCs (p < 0.001) and HCs (p = 0.049). A significantly lower binding level of ConA (preferred mannose) was observed in patients with aneurysm diameter >5 cm. Significantly higher binding of CSA (preferred GalNAc) was present for dyslipidemia patients, whereas a lower binding level of AAL (preferred fucose) was observed for hypertensive patients. Patients with diabetes had lower binding levels of IRA (preferred GalNAc) and HPA (preferred GalNAc) compared with those not with DM. PTL-L (R = 0.36, p = 0.0015, preferred GalNAc) was positively associated with aneurysm diameters, whereas DSL (R = 0.28, p = 0.014, preferred (GlcNAc)2-4) was positively associated with patients’ age. Symptomatic patients had a lower binding level of ConA (p = 0.032), and patients with coronary heart disease had higher binding levels of STL (p = 0.0029, preferred GlcNAc). Patients with ILT bound less with black bean crude (p = 0.04, preferred GalNAc). Conclusions: AAA was associated with a decreased IgG binding level of SBA (recognizing glycan GalNAc). Symptomatic patients with aneurysm <5 cm had a higher binding level of ConA (preferred mannose). Coronary heart disease and elder age were associated with increased IgG bisecting GlcNAc. IgG O-glycosylation (GalNAc) may play an important role in AAA pathogenesis and progression.
Ureteral Access Sheaths and Its Use in the Future: A Comprehensive Update Based on a Literature Review
Authors: De Coninck Vincent , Somani Bhaskar , Sener Emre Tarik , Emiliani Esteban , Corrales Mariela , Juliebø-Jones Patrick , Pietropaolo Amelia , Mykoniatis Ioannis , Zeeshan Hameed Belthangady M. , Esperto Francesco , Proietti Silvia , Traxer Olivier , Keller Etienne Xavier
Published: September 2022

Ureteral access sheaths (UASs) are part of urologist’s armamentarium when performing retrograde intrarenal surgery (RIRS). Recently, the world of RIRS has changed dramatically with the development of three game-changers: thulium fiber laser (TFL), smaller size single use digital flexible ureterosopes and intraoperative intrarenal pressure (IRP) measurement devices. We aimed to clarify the impact of UASs on IRP, complications and SFRs and put its indications in perspective of these three major technological improvements. A systematic review of the literature using the Medline, Scopus and Web of Science databases was performed by two authors and relevant studies were selected according to PRISMA guidelines. Recent studies showed that using a UAS lowers IRP and intrarenal temperature by increasing irrigation outflow during RIRS. Data on the impact of a UAS on SFRs, postoperative pain, risk of infectious complications, risk of ureteral strictures and risk of bladder recurrence of urothelial carcinoma after diagnostic RIRS were inconclusive. Prestenting for at least one week resulted in ureteral enlargement, while the influence of pre-operative administration of alpha-blockers was unclear. Since TFL, smaller single use digital ureteroscopes and devices with integrated pressure-measuring and aspiration technology seemed to increase SFRs and decrease pressure and temperature related complications, indications on the use of a UAS may decrease in the near future.
Corneal Confocal Microscopy as a Quantitative Imaging Biomarker of Diabetic Peripheral Neuropathy: A Review
Authors: Cosmo Eleonora , Midena Giulia , Frizziero Luisa , Bruno Marisa , Cecere Michela , Midena Edoardo
Published: September 2022

Distal symmetric polyneuropathy (DPN), particularly chronic sensorimotor DPN, represents one of the most frequent complications of diabetes, affecting 50% of diabetic patients and causing an enormous financial burden. Whilst diagnostic methods exist to detect and monitor this condition, they have significant limitations, mainly due to their high subjectivity, invasiveness, and non-repeatability. Corneal confocal microscopy (CCM) is an in vivo, non-invasive, and reproducible diagnostic technique for the study of all corneal layers including the sub-basal nerve plexus, which represents part of the peripheral nervous system. We reviewed the current literature on the use of CCM as an instrument in the assessment of diabetic patients, particularly focusing on its role in the study of sub-basal nerve plexus alterations as a marker of DPN. CCM has been demonstrated to be a valid in vivo tool to detect early sub-basal nerve plexus damage in adult and pediatric diabetic patients, correlating with the severity of DPN. Despite its great potential, CCM has still limited application in daily clinical practice, and more efforts still need to be made to allow the dissemination of this technique among doctors taking care of diabetic patients.
Manganese Superoxide Dismutase as a Novel Oxidative Stress Biomarker for Predicting Paroxysmal Atrial Fibrillation
Authors: Liu Hao , Wang Qiao , Liu Daiqi , Li Ziqi , Fu Yulin , Tse Gary , Li Guangping , Liu Tong , Xu Gang
Published: September 2022

Background: There is accumulating evidence indicating that inflammation and oxidative stress are involved in the pathogenesis of atrial fibrillation (AF). The role of manganese superoxide dismutase (MnSOD) in the initiation and maintenance of AF has not yet been well characterized. The aim of our study is to investigate whether or not plasma MnSOD levels are associated with AF. Methods: We enrolled a total of 130 consecutive patients with AF as the case group (paroxysmal AF: 87, persistent AF: 43) and 58 patients without a history of AF as the control group after screening. Baseline clinical characteristics, laboratory and echocardiographic parameters were collected. Plasma levels of nicotinamide-adenine dinucleotide phosphate oxidase 4 (NOX4) and MnSOD were measured by an enzyme-linked immunosorbent assay (ELISA) method. These data were compared between the different groups. The relationship between MnSOD and other parameters was assessed using Spearman correlation. Multivariable logistic regression analysis was performed to identify independent predictors of AF. The area under the curve (AUC) from receiver operating characteristics (ROC) analysis was constructed to explore the value of MnSOD in predicting the occurrence of AF. Results: The levels of MnSOD were the highest in the paroxysmal AF group, followed by the persistent AF group, and the lowest in the controls. Meanwhile, the levels in the paroxysmal AF group were significantly higher than those in the controls [322.84 (165.46, 547.61) vs. 201.83 (129.53, 301.93), p = 0.002], but no significant difference was found between the paroxysmal AF group and persistent AF group, as well as the persistent AF group and the controls. Spearman correlation analysis indicated that there was a significantly negative correlation between MnSOD levels and LAD (r = −0.232, p = 0.008) and a positive correlation between MnSOD levels and RDW-CV (r = 0.214, p = 0.014) in the case group. Multivariate logistic regression analysis indicated that MnSOD levels [odds ratio (OR): 1.003, 95% confidence interval (CI): 1.001–1.005, p = 0.002] were an independent risk factor for paroxysmal AF, and the best cut-off value of MnSOD in predicting paroxysmal AF gained by ROC curve analysis was 311.49 ug/mL (sensitivity of 52.9%, specificity of 77.6%, AUC = 0.668). Conclusion: Oxidative stress underlies the pathogenesis of AF and may play a stronger role in paroxysmal AF than persistent AF. Our study showed an independent association between increased circulating plasma MnSOD levels and the occurrence of paroxysmal AF.
Effects of Different Pressure Levels in Topical Negative Pressure Application—Analysis of Perfusion Parameters in a Clinical Skin Model Using Multimodal Imaging Techniques
Authors: Aslan-Horch Emine Ceylan , Horch Raymund E. , Arkudas Andreas , Müller-Seubert Wibke , Ludolph Ingo
Published: September 2022

The effects of topical negative pressure therapy (TNP) have been a subject of research for many years. In this study, we investigated new imaging devices to detect clinical changes that TNP causes on healthy tissue and identified differences in microcirculation created by different pressure levels. We used near-infrared spectroscopy (NIS), thermography, and a vein illuminator to measure the differences in oxygen saturation, tissue temperature, and vein pattern. A control group (−125 mmHg) and three comparison groups with only TNP dressing (Group 1), −25 mmHg (Group 2), and −175 mmHg (Group 3) were established. Thirty minutes of TNP on intact skin was followed by 30 min of resting. A total of 24 participants were measured by all imaging devices at predetermined time points. Oxygen saturation and skin temperature increased by 8.07% and 1.67 °C for the control group, 4.00% and 1.65 °C for Group 2, and 8.45% and 1.68 °C for Group 3. Group 1 showed a slight increase in oxygen saturation and a 2.7 °C increase in skin temperature. Over the 30 min following removal of TNP, oxygen saturation and temperature decreased gradually for all groups. The vein illuminator did not show significant differences in the venous pattern or flow. Our study showed that higher negative pressure values resulted in higher oxygen saturation and higher tissue temperature.
Potential Risk of Overtreatment in Patients with Type 2 Diabetes Aged 75 Years or Older: Data from a Population Database in Catalonia, Spain
Authors: Mata-Cases Manel , Mauricio Didac , Real Jordi , Vlacho Bogdan , Romera-Liebana Laura , Molist-Brunet Núria , Cedenilla Marta , Franch-Nadal Josep
Published: September 2022

Aim: To assess the potential risk of overtreatment in patients with type 2 diabetes (T2DM) aged 75 years or older in primary care. Methods: Electronic health records retrieved from the SIDIAP database (Catalonia, Spain) in 2016. Variables: age, gender, body mass index, registered hypoglycemia, last HbA1c and glomerular filtration rates, and prescriptions for antidiabetic drugs. Potential overtreatment was defined as having HbA1c < 7% or HbA1c < 6.5% in older patients treated with insulin, sulfonylureas, or glinides. Results: From a total population of 138,374 T2DM patients aged 75 years or older, 123,515 had at least one HbA1c available. An HbA1c below 7.0% was present in 59.1% of patients, and below 6.5% in 37.7%. Overall, 23.0% of patients were treated with insulin, 17.8% with sulfonylureas, and 6.6% with glinides. Potential overtreatment (HbA1c < 7%) was suspected in 26.6% of patients treated with any high-risk drug, 47.8% with sulfonylureas, 43.5% with glinides, and 28.1% with insulin. Using the threshold of HbA1c < 6.5%, these figures were: 21.6%, 24.4%, 17.9%, and 12.3%, respectively. Conclusion: One in four older adults with T2DM treated with antidiabetic drugs associated with a high risk of hypoglycemia might be at risk of overtreatment. This risk is higher in those treated with sulfonylureas or glinides than with insulin.
Use of Melatonin and/on Ramelteon for the Treatment of Insomnia in Older Adults: A Systematic Review and Meta-Analysis
Authors: Marupuru Srujitha , Arku Daniel , Campbell Ashley M. , Slack Marion K. , Lee Jeannie K.
Published: September 2022

To investigate the efficacy of melatonin and/or ramelteon reporting sleep outcomes for older adults with chronic insomnia, a systematic review and a meta-analysis of PubMed, EMBASE, Cochrane library, International Pharmaceutical Abstracts, PsycINFO, science citation index, center for reviews and dissemination, CINAHL, grey literature and relevant sleep journal searches were conducted from 1 January 1990 to 20 June 2021. Randomized controlled trials and other comparative studies with melatonin and/or ramelteon use among older patients with chronic insomnia were included. Funnel plot and Egger’s test was used to determine publication bias. A forest plot was constructed to obtain a pooled standardized mean difference using either a fixed or random effects model for each of the two broad categories of sleep outcomes: objective and subjective. Of 5247 studies identified, 17 studies met the inclusion criteria for MA. Study sample size ranged from 10 to 829 with the mean age ≥55 years. There were significant improvements in total sleep time (objective), sleep latency and sleep quality (objective and subjective) for melatonin and/or ramelteon users compared with placebo. Sleep efficiency was not significantly different. The effects of these agents are modest but with limited safe treatment options for insomnia in older adults, these could be the drugs of choice.
Advances in the Non-Operative Management of Multidirectional Instability of the Glenohumeral Joint
Authors: Watson Lyn , Pizzari Tania , Balster Simon , Lenssen Ross , Warby Sarah Ann
Published: September 2022

Multidirectional instability (MDI) of the glenohumeral joint refers to symptomatic subluxations or dislocations in more than one direction. The aetiology of MDI is multifactorial, which makes the classification of this condition challenging. A shoulder rehabilitation program is the initial recommended treatment for MDI, however available rehabilitation programs have varying levels of evidence to support their effectiveness. In 2016, we published the details of an evidence-based program for MDI that has been evaluated for efficacy in two single-group studies and a randomised controlled trial. In 2017, we published a clinical commentary on the aetiology, classification, and treatment of this condition. The aim of this paper is to provide an update on the components of these publications with a particular focus on new advances in the non-operative management of this condition.
Comparison of the Treatment Efficacy of Rosuvastatin versus Atorvastatin Loading Prior to Percutaneous Coronary Intervention in ST-Segment Elevation Myocardial Infarction
Authors: Adel Esraa M. , Elberry Ahmed A. , Abdel Aziz Ahmed , Naguib Ibrahim A. , Alghamdi Badrah S. , Hussein Raghda R. S.
Published: September 2022

The aim of this study was to compare the effect of a single high-dose rosuvastatin versus atorvastatin preloading in ST-elevation myocardial infarction (STEMI) patients receiving primary percutaneous coronary intervention (PCI.) Methods: A total of 99 patients presented with STEMI and were randomly divided into three groups—a control group (n = 33) with no statin treatment, an atorvastatin group (n = 33) with a single 80 mg atorvastatin dose and the rosuvastatin group (n = 33) with a single 40 mg rosuvastatin dose in the emergency room (ER) prior to PCI. Post-interventional thrombolysis in myocardial infarction (TIMI) flow grade and corrected TIMI frame count (CTFC) were recorded, and ST-segment resolution was measured. Results: CTFC was significantly lower for the atorvastatin group (p-value < 0.01) than in the control group. A final TIMI flow grade 3 was achieved in 32 (97.0%) patients in the rosuvastatin group and 28 (84.8%) patients in the atorvastatin group compared with only 25 (75.8%) patients in the control group (p = 0.014). Peak CK-MB in the rosuvastatin group (263.2 [207.2–315.6]) and the atorvastatin group (208 [151.0–314.1]) was lower compared to that in the control group (398.4 [303.9–459.3]); p < 0.001. Conclusions: A single extensive dose of lipophilic atorvastatin prior to primary PCI in STEMI patients showed better improvement in microvascular myocardial perfusion compared to hydrophilic rosuvastatin.
Nonalcoholic Fatty Liver Disease and Its Complex Relation with Type 2 Diabetes Mellitus—From Prevalence to Diagnostic Approach and Treatment Strategies
Authors: Diaconu Cosmina-Theodora , Guja Cristian
Published: September 2022

Prevalence of Nonalcoholic Fatty Liver Disease (NAFLD) and Type 2 Diabetes Mellitus (T2DM) are increasing rapidly worldwide, reaching epidemic proportions. Their association, based on common metabolic risk factors (obesity, insulin resistance (IR), unhealthy lifestyle), brings an additional risk of both hepatic and cardiovascular (CV) adverse clinical outcomes. The terminology of “NAFLD” is stigmatizing to some but not all patients, and a more practical one should be announced soon. Medical strategies can address both diseases simultaneously, as they have crossing pathophysiological mechanisms, mainly IR. Strategies vary from lifestyle intervention and pharmacological options, as more molecules designated for T2DM treatment may be helpful in NAFLD, to surgical procedures. This review focuses on the coexistence of NAFLD and T2DM, pointing out the utility of the appropriate terminology, its prevalence, and mortality rates among the diabetic population. Briefly, we have discussed the main pathophysiological mechanisms and the risk stratification algorithm for the development of NAFLD and nonalcoholic steatohepatitis (NASH) as well as the tools for evaluation of fibrosis. Finally, we have focused on the current therapeutic options for the treatment of NAFLD associated with T2DM.