bluuebunny/crossref_metadata_2025 huggingface dataset:
Post-Treatment Thyroid Diseases in Children with Brain Tumors: A Single-Center Experience at “Prof. Dr. Ion Chiricuță” Institute of Oncology, Cluj-Napoca
Authors: Cosnarovici Maria Margareta , Piciu Andra , Bonci Eduard-Alexandru , Bădan Marius-Ioan , Bădulescu Claudiu-Iulian , Stefan Andreea-Ioana , Mester Alexandru , Cosnarovici Rodica , Larg Maria-Iulia
Published: March 2020

Aim of study: The purpose of the study was to evaluate the association of thyroid dysfunction occurring in pediatric patients treated for brain tumors. Patients and methods: A total of 255 patients with brain tumors were treated between 2001 and 2018 at the “Prof. Dr. Ion Chiricuță” Institute of Oncology, Cluj-Napoca. Due to a minimum follow-up of 4 years, we studied 184 out of the 255 patients. The cohort included 69 girls (37.5%) and 109 boys (62.5%), with a median age of 8.4 years. The evaluated tumors included medulloblastomas (47 patients), astrocytomas (44 patients), ependymomas (22 patients), gliomas (20 patients), germ cell tumors (12 patients), primitive neuroectodermal tumors (4 patients), as well as other types of tumors (15 patients); in 20 of the cases, biopsy could not be performed. Results: There was a 60% overall survival rate; among the 120 surviving patients, 11 (9.1%) were diagnosed with iatrogenic thyroid disease. We observed an important number of iatrogenic thyroid disease cases in this group of patients, thus revealing the importance of long-term thyroid function evaluation in all children who finalized their treatment for brain tumors. Through this study, we aimed to provide an accurate image of the methodology of monitoring for thyroid dysfunction in childhood brain tumor survivors. Conclusion: Given the fact that the probability of developing thyroid dysfunction in the pediatric population treated for brain tumors is not rare, we recommend that childhood brain tumor survivors be monitored for iatrogenic thyroid disease, in order to provide early diagnosis and treatment.
Runge-Kutta Model Predictive Speed Control for Permanent Magnet Synchronous Motors
Authors: Akpunar Adile , Iplikci Serdar
Published: March 2020

Permanent magnet synchronous motors (PMSMs) have commonly been used in a wide spectrum ranging from industry to home appliances because of their advantages over their conventional counterparts. However, PMSMs are multiple-input multiple-output (MIMO) systems with nonlinear dynamics, which makes their control relatively difficult. In this study, a novel model predictive control mechanism, which is referred to as the Runge-Kutta model predictive control (RKMPC), has been applied for speed control of a commercial permanent magnet synchronous motor. Furthermore, the RKMPC method has been utilized for the adaptation of the speed of the motor under load variations via RKMPC-based online parameter estimation. The superiority of RKMPC is that it can take the constraints on the inputs and outputs of the system into consideration, thereby handling the speed and current control in a single loop. It has been shown in the study that the RKMPC mechanism can also estimate the load changes and unknown load disturbances to eliminate their undesired effects for a desirable control accuracy. The performance of the employed mechanism has been tested on a 0.4 kW PMSM motor experimentally for different conditions and compared to the conventional Proportional Integral (PI) method. The tests have shown the efficiency of RKMPC for PMSMs.
Addressing Environmental Change through Emergent Integrated Environmental Observatories: A Case Study in the Czech Republic
Authors: Acosta Manuel , Ač Alexander , Pavelka Marian , Havránková Kateřina , Loescher Henry W. , Butler James H. , Janouš Dalibor , Marek Michal V.
Published: March 2020

A growing body of scientific evidence indicates that we have entered the Anthropocene Epoch. Many assert that society has exceeded sustainable ecological planetary boundaries and that altered biogeophysical processes are no longer reversible to natural rates of ecosystem functioning. To properly and successfully address societal needs for the future, more holistic and complex methods need to be applied at various spatial and temporal scales. The increasingly interconnected nature of human and natural environments—from individuals to large megacities and entire continents and from cells through ecosystems to the biosphere as a whole (e.g., as seen in the carbon cycle)—demand new and often interdisciplinary and international approaches to address emerging global challenges. With that perspective in mind, the Czech Republic’s National Climate Program was established in 1991 with the aim to understand the impact of global environmental change on society. The National Climate Program was updated in 2017 to formulate a new Climate Protection Policy. Here, we outline the multifaceted problems that climate change poses for the Czech Republic, as well as a new scientific infrastructure and approaches directed to better understanding the effects of climate change on our ecosystems, water resources, urban environment, agriculture, human health, and general economy.
Towards Forest Landscape Restoration Programs in the Philippines: Evidence from Logged Forests and Mixed-Species Plantations
Authors: Veridiano Rizza Karen , Schröder Jobst Michael , Come Renezita , Baldos Angelica , Günter Sven
Published: March 2020

With only 7.01 million hectares of remaining forested areas in the Philippines, there is an urgency to protect these areas, while also implementing restoration strategies to increase forest cover and improve forest functionality. In this study, we assess how the so called “rainforestation” approach, attempts to implement close-to-nature restoration strategies in humid tropic areas. One of the main objectives of the “rainforestation” approach as a form of a mixed-species plantation is the rehabilitation of structural and floristic integrity similar to natural conditions. We compared study areas located in the provinces of Leyte and Southern Leyte composed of logged forests (temporary plots), with logged forests on a permanent research plot and with mixed-species plantations. Basal area, carbon stocks, volume and biological diversity between study areas were calculated and compared, both for static and dynamic data. Results from the static data indicate that carbon stocks (89.30 t ha−1) and volume (262.56 m3 ha−1) of the mixed-species plantations (“rainforestation” approach) is significantly lower than that of the logged forests. However, when it comes to the capacity of the study areas for potential increments, the mixed-species plantations are not significantly different on basal area increment (0.99 m2 ha−1 yr−1), carbon stock increment (3.67 t ha−1 yr−1) and total volume increment (10.47 m3 ha−1 yr−1) as compared to the logged forests’ capacity (basal area—1.08 m2 ha−1 yr−1, carbon—4.06 t ha−1 yr−1 and total volume—11.98 m3 ha−1 yr−1). The species composition was only partly comparable to logged forests of the region, but overall tree species richness is high in comparison to classical plantation approaches. Previously logged forests are able to recover fast reaching surprisingly high values of carbon stocks and potential commercial timber volume. Our study indicates that “rainforestation” cannot fully replace the functionality of natural forests, but can provide a surprisingly multifunctional tool for landscape restoration, providing both timber and non-timber ecosystem services.
High Nitrate or Ammonium Applications Alleviated Photosynthetic Decline of Phoebe bournei Seedlings under Elevated Carbon Dioxide
Authors: Wang Xiao , Wei Xiaoli , Wu Gaoyin , Chen Shenqun
Published: March 2020

Phoebe bournei is a precioustimber species and is listed as a national secondary protection plant in China. However, seedlings show obvious photosynthetic declinewhen grown long-term under an elevated CO2 concentration (eCO2). The global CO2 concentration is predicted to reach 700 μmol·mol−1 by the end of this century; however, little is known about what causes the photosynthetic decline of P. bournei seedlings under eCO2 or whether this photosynthetic decline could be controlled by fertilization measures. To explore this problem, one-year-old P. bournei seedlings were grown in an open-top air chamber under either an ambient CO2 (aCO2) concentration (350 ± 70 μmol·mol−1) or an eCO2 concentration (700 ± 10 μmol·mol−1) from June 12th to September 8th and cultivated in soil treated with either moderate (0.8 g per seedling) or high applications (1.2 g per seedling) of nitrate or ammonium. Under eCO2, the net photosynthetic rate (Pn) of P. bournei seedlings treated with a moderate nitrate application was 27.0% lower than that of seedlings grown under an aCO2 concentration (p < 0.05), and photosynthetic declineappeared to be accompanied by a reduction of the electron transport rate (ETR), actual photochemical efficiency, chlorophyll content, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), rubisco activase (RCA) content, leaf thickness, and stomatal density. The Pn of seedlings treated with a high application of nitrate under eCO2 was 5.0% lower than that of seedlings grown under aCO2 (p > 0.05), and photosynthetic declineoccurred more slowly, accompanied by a significant increase in rubisco content, RCA content, and stomatal density. The Pn of P. bournei seedlings treated with either a moderate or a high application of ammonium and grown under eCO2 was not significantly differentto that of seedlings grown under aCO2—there was no photosynthetic decline—and the ETR, chlorophyll content, rubisco content, RCA content, and leaf thickness values were all increased. Increasing the application of nitrate or the supply of ammonium could slow down or prevent the photosynthetic declineof P. bournei seedlings under eCO2 by changing the leaf structure and photosynthetic physiological characteristics.
Need for Pre-Harvest Clearing of Understory Vegetation Determined by Airborne Laser Scanning
Authors: Sanz Blanca , Malinen Jukka , Heiskanen Jussi , Tokola Timo
Published: March 2020

The methodology presented here can assist in evaluating the need for pre-harvest clearing. In the long term, similar approaches may help with managing electronic standing sales and enhance the operational environment of roundwood e-marketplaces. In cut-to-length harvesting, pre-harvest clearing is needed when the understory vegetation hinders the visibility of the stems to be harvested. It can facilitate the work of the harvester operators and thereby enhance the productivity and quality of the harvesting operation. Information about where pre-harvest clearing is required is often not available, however, or else it has to be collected during time-consuming field visits. We report here on the development and evaluation of airborne laser scanning (ALS)-based models for estimating the need for pre-harvest clearing. The reference data consisted of 99 circular field sample plots that were photographed and in which stems with diameters at breast height from one to seven centimeters were measured. An online e-questionnaire survey responded to by 66 forest professionals classified the sample plots into five categories ranging from no need for pre-harvest clearing to compulsory pre-harvest clearing. A linear discriminant analysis was used to estimate the need for pre-harvest clearing with an accuracy of 63.6%, whereas a linear model-based method that predicted the understory stem density assessed the need with an accuracy of 64.6%. Use of this method could deliver information about the understory vegetation, offer guidelines for clearing the understory, and reduce the number of field visits before harvesting, thus reducing costs.
Children’s Literature and the Holocaust
Authors: Giambastiani Verbena
Published: March 2020

The aim of my paper is to examine children’s literature written in Italy and centred on the Holocaust. It is quite common for people to deem the subject matter inappropriate for young audiences, whilst it is also considered disrespectful to write inventive literature for children about the death camps. Nevertheless, it seems necessary to inform children about such a major historical event. Moreover, the stories written on this subject aim to introduce children to themes like prejudice, discrimination and racism. My research focuses on the recurrent patterns that occur frequently in these books. In these books, the focus lies on the victims rather than the perpetrators. They deal with the story of a Jewish family and frequently feature a child as the protagonist. These books will undoubtedly provoke questions by young readers, but they are most likely best read with an adult who can answer any questions appropriately and deepen the historical frame. These narratives are important because educators have a responsibility to teach others and read about the Holocaust.
South Sudanese Refugee Survivors of Sexual and Gender-Based Violence and Torture: Health and Justice Service Responses in Northern Uganda
Authors: Liebling Helen , Barrett Hazel , Artz Lilly
Published: March 2020

This British Academy/Leverhulme-funded research investigated the health and justice service responses to the needs of South Sudanese refugees living in refugee settlements in Northern Uganda who had been subjected to sexual and gender-based violence (SGBV) and torture. It involved the collection and thematic analysis of the narratives of 20 men and 41 women who were refugee survivors of SGBV and torture, including their experiences in South Sudan, their journeys to Uganda and experiences in refugee settlements, in particular their access to health and justice services. Thirty-seven key stakeholders including international, government, non-government organisations and civil society organisations were also interviewed regarding their experiences of providing health and justice services to refugees. All refugees had survived human rights abuses mainly carried out in South Sudan but some had also occurred on route to Uganda and within Uganda. Despite the significant impact of their experiences, the analysis indicated that there was limited service response in refugee settlements in Northern Uganda once the immediate humanitarian crisis ended. The thematic analysis indicated five main themes coming from the interviews. These included: the nature of refugee experiences of SGBV and torture, including domestic violence and child abduction and forced marriage; issues associated with service provision such as lack of adequate screening and under resourcing of health and justice services; a lack of gender sensitivity and specialist services, particularly for men; the sustained involvement of civil society organisations and local non-governmental organisations in providing counselling and offering emotional support and hope to survivors; and enhancing health and justice responses and services to improve refugee recovery, dignity and resilience. The authors recommend that integrated gendered and culturally sensitive service provision should be adopted, which brings together formal and informal health, justice services and survivor support programmes.
Factors Differentiating the Level of Functional Fitness in Polish Seniors
Authors: Umiastowska Danuta , Kupczyk Joanna
Published: March 2020

In this study, functional fitness is defined as the level of independence and self-sufficiency of an elderly person, which facilitates leading an independent life, without the need for assistance from other people. A decrease in functional fitness among older adults is influenced by a variety of factors. In addition to changes occurring in the human body in accordance with the laws of ontogenetic development, they can also be analyzed in terms of somatic parameters and the age of the subjects. The aim of this research was to find the factors differentiating the level of functional fitness of older adults. It involved 509 people divided into a group of people involved in regular physical activity and an inactive group. The Fullerton Functional Fitness Test was used to measure the level of physical fitness, and anthropometric measurements (body weight and height) were also performed. The level of functional fitness of Polish seniors was compared with the American standards established by R. E. Rikli and C. J. Jones. The results of our research confirm a higher level of functional fitness in active older adults, both women and men. In this group, BMI—(Body Mass Index is a measure of body fat based on height and weight) showed a greater correlation with shoulder girdle and back muscles strength, complex coordination, balance, agility, and endurance in women than in men. Finally, the surveyed Polish seniors exhibited a higher level of functional fitness than their American peers.
Longitudinal Analysis of Plantar Pressures with Wear of a Running Shoe
Authors: Escamilla-Martínez Elena , Gómez-Martín Beatriz , Fernández-Seguín Lourdes María , Martínez-Nova Alfonso , Pedrera-Zamorano Juan Diego , Sánchez-Rodríguez Raquel
Published: March 2020

Running shoes typically have a lifespan of 300–1000 km, and the plantar pressure pattern during running may change as the shoe wears. So, the aim of this study was to determine the variation of plantar pressures with shoe wear, and the runner’s subjective sensation. Maximun Plantar Pressures (MMP) were measured from 33 male recreational runners at three times during a training season (beginning, 350 km, and 700 km) using the Biofoot/IBV® in-shoe system (Biofoot/IBV®, Valencia, Spain). All the runners wore the same shoes (New Balance® 738, Boston, MA, USA) during this period, and performed similar training. The zones supporting most pressure at all three study times were the medial (inner) column of the foot and the forefoot. There was a significant increase in pressure on the midfoot over the course of the training season (from 387.8 to 590 kPa, p = 0.003). The runners who felt the worst cushioning under the midfoot were those who had the highest peak pressures in that area (p = 0.002). The New Balance® 738 running shoe effectively maintains the plantar pressure pattern after 700 km of use under all the zones studied except the midfoot, probably due to material fatigue or deficits of the specific cushioning systems in that area.
Understanding the Green Development Behavior and Performance of Industrial Enterprises (GDBP-IE): Scale Development and Validation
Authors: Li Xingwei , Du Jianguo , Long Hongyu
Published: March 2020

Industrial enterprises have provided outstanding contributions to economic development in countries around the world. The green development of industrial enterprises has received widespread attention from researchers. However, existing research lacks the tools to scientifically measure the green development behavior and performance of industrial enterprises. According to the theory of green development behavior and performance of industrial enterprises (GDBP-IE), the aim of this paper is to provide a tool for scientifically measuring such behavior and performance. This paper determined the initial scale through literature analysis and expert discussions and obtained valid samples from 31 provincial administrative regions in China through field and online surveys (N = 853). The exploratory factor analysis method was used to test the reliability and validity of the scale. The main conclusions are as follows: (1) The reliability and validity of the GDBP-IE scale are good; (2) the GDBP-IE scale, with a total of 70 items, comprises four sub-scales: The internal factors sub-scale, the external factors sub-scale, the green development behavior of industrial enterprises sub-scale, and the green development performance of industrial enterprises sub-scale. Among them, the internal factors sub-scale, with a total of 13 items, consists of two dimensions: Corporate tangible resources and corporate intangible resources. The external factors sub-scale, with a total of 23 items, consists of three dimensions: Market environment; public supervision; policy and institutional environment. The green development behavior of industrial enterprises sub-scale, with a total of 18 items, consists of two dimensions: Clean production behavior and green supply chain management practice. The green development performance of industrial enterprises sub-scale, with a total of 16 items, comprises three dimensions: Corporate social performance, corporate financial performance, and corporate environmental performance. The findings enrich the research on corporate organizational behavior, green behavior, and green development system theory, and provide tools for further empirical testing. The development and verification of green development behavior and performance of industrial enterprises can help guide various types of industrial enterprises in transforming to green development and can provide a reference for the government to formulate targeted green development policies.
Development and Validation of an Atrial Fibrillation Knowledge Assessment Tool (AFKAT)
Authors: Jatau Abubakar Ibrahim , Wimmer Barbara C. , Bereznicki Luke R. , Dwan Corinna , Black J. Andrew , M. Bezabhe Woldesellassie , M. Peterson Gregory
Published: March 2020

Assessing and improving public knowledge of atrial fibrillation (AF) could increase its detection rate and the subsequent use of stroke prevention therapies. However, there is no validated AF knowledge assessment tool applicable to the general population, including those at risk of AF. Therefore, we aimed to develop and validate such a tool. The tool was developed from a literature review and discussion with subject matter experts. Content validity was ensured by a ten-member panel of experts comprising cardiologists and pharmacists. An online validation survey was conducted and reported based on the Checklist for Reporting Results of Internet E-Surveys (CHERRIES). The survey evaluated the tool performance by construct validity, internal consistency reliability, item discrimination, difficulty index and ease of readability. The survey participants included 14 general medical specialists, 20 fourth-year and 33 second-year undergraduate pharmacy students, and 122 members of the general public. The tool had satisfactory content validity, with a scale content validity index of 0.8. The mean percentage knowledge scores for general medical specialists and fourth-year pharmacy students were higher than second-year pharmacy students, followed by the general public (92.9%, 87.6%, 68.5% and 53.4%, respectively; p-value < 0.001), supporting construct validity. The tool had good internal consistency reliability (Cronbach’s alpha = 0.91). The item-total correlation was in the preferred range of 0.23 to 0.71. The Atrial Fibrillation Knowledge Assessment Tool is a valid instrument and can be used to investigate AF knowledge of the general population.
Investigating the Impact of Climate Warming on Phenology of Aphid Pests in China Using Long-Term Historical Data
Authors: Wu Yangxue , Li Junjie , Liu Huanhuan , Qiao Gexia , Huang Xiaolei
Published: March 2020

Global climate warming has significant influence on individual development, population dynamics, and geographical distribution of many organisms, which has drawn much attention in recent years. As a large group of poikilotherms, insects whose life activities are closely linked with ambient temperature are supposed to be influenced by global warming. In order to test the consistency or difference of the effects of long-term climate warming on phytophagous insect pests in different geographical environments, this study collected historical data on the occurrence and population dynamics of three aphid pests (Myzus persicae, Aphis gossypii, and Sitobion avenae) in China, and systematically explored their phenological responses. We found that, during a period of about 60 years, in general, the first occurrence dates and the first migration dates of the three aphids almost moved earlier, while the end of the occurrence and the last migration dates were slightly delayed. However, these responses also represented geographical variation at a local scale. Basically, our results showed that the occurrence and migration seasons of these three aphid pests have been prolonged along with climate warming. This study based on historical literature data provides empirical evidence and valuable implications for understanding the impact of climate warming on insect pests and future management strategies.
Approximate Depth Shape Reconstruction for RGB-D Images Captured from HMDs for Mixed Reality Applications
Authors: Awano Naoyuki
Published: March 2020

Depth sensors are important in several fields to recognize real space. However, there are cases where most depth values in a depth image captured by a sensor are constrained because the depths of distal objects are not always captured. This often occurs when a low-cost depth sensor or structured-light depth sensor is used. This also occurs frequently in applications where depth sensors are used to replicate human vision, e.g., when using the sensors in head-mounted displays (HMDs). One ideal inpainting (repair or restoration) approach for depth images with large missing areas, such as partial foreground depths, is to inpaint only the foreground; however, conventional inpainting studies have attempted to inpaint entire images. Thus, under the assumption of an HMD-mounted depth sensor, we propose a method to inpaint partially and reconstruct an RGB-D depth image to preserve foreground shapes. The proposed method is comprised of a smoothing process for noise reduction, filling defects in the foreground area, and refining the filled depths. Experimental results demonstrate that the inpainted results produced using the proposed method preserve object shapes in the foreground area with accurate results of the inpainted area with respect to the real depth with the peak signal-to-noise ratio metric.
One-Pot Decoration of Cupric Oxide on Activated Carbon Fibers Mediated by Polydopamine for Bacterial Growth Inhibition
Authors: Moon Hangil , Lee Young-Chul , Hur Jaehyun
Published: March 2020

Despite the widespread application of activated carbon fiber (ACF) filters in air cleaning owing to their high surface area and low price, they have certain limitations in that they facilitate bacterial growth upon prolonged use as ACF filters can provide favorable conditions for bacterial survival. The deposition of cupric oxide (CuO) on ACFs can be an effective way of resolving this problem because CuO can inhibit the proliferation of bacteria owing to its antimicrobial properties. However, finding a new method that allows the simple and uniform coating of CuO on ACF filters is challenging. Here, we demonstrate one-pot CuO deposition mediated by polydopamine (PD) to realize an ACF filter with antimicrobial activity. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS) analyses reveal that CuO and PD are uniformly deposited on the ACF surface. The amount of CuO formed on the ACFs is measured by thermogravimetric analysis (TGA). Finally, the changes in surface area, pressure drop, and antimicrobial activity after coating PD-CuO on the ACFs are evaluated. The use of PD-CuO on the ACFs effectively suppresses the growth of bacteria and enhances the mechanical properties without significantly sacrificing the original characteristics of the ACF filter.
Effect of Carbonaceous Components on Tribological Properties of Copper-Free NAO Friction Material
Authors: Lin Hsun-Yu , Cheng Huy-Zu , Lee Kuo-Jung , Wang Chih-Feng , Liu Yi-Chen , Wang Yu-Wei
Published: March 2020

Copper helps to accelerate heat transfer during the braking process, allowing the brake materials to produce a stable coefficient of friction (COF), which in turn reduces wear loss and braking noise. However, its properties are also quite harmful to aquatic organisms. Finding a suitable replacement that fits all functions of copper for brake materials is not an easy feat. In this paper, six different carbonaceous components (coke, carbon black, carbon fiber, artificial graphite, natural graphite and expanded graphite) were substituted for copper in non-asbestos organic (NAO) friction materials. The hardness, thermal conductivity and tribological behaviors of these copper-free NAO friction materials were examined. Experimental results indicate that carbonaceous components improve lubrication and assist the friction composites with generating friction layers on the worn surface. Specimens containing coke, carbon black or carbon fiber exhibit broken friction layers, whereas specimens containing artificial graphite, natural graphite or expanded graphite exhibit quite adherent and smooth friction layers. Among all the copper-free carbon containing specimens, the specimen containing expanded graphite appears to be the best choice. It has the highest thermal conductivity, a relatively low wear loss and a relatively high and stable COF.
Influence of Solidification Conditions on the Microstructure of Laser-Surface-Melted Ductile Cast Iron
Authors: Janicki Damian , Górka Jacek , Kwaśny Waldemar , Pakieła Wojciech , Matus Krzysztof
Published: March 2020

The thermal conditions in the molten pool during the laser surface melting of ductile cast iron EN-GJS-700-2 were estimated by using infrared thermography and thermocouple measurements. The thermal data were then correlated with the microstructure of the melted zone. Additionally, the thermodynamic calculations of a Fe-C-Si alloy system were performed to predict the solidification path of the melted zone. It was found that increasing the cooling rate during solidification of the refined ledeburite eutectic but also suppressed the martensitic transformation. A continuous network of plate-like secondary cementite precipitates and nanometric spherical precipitates of tertiary cementite were observed in regions of primary and eutectic austenite. The solidification of the melted zone terminated with the Liquid → γ-Fe + Fe3C + Fe8Si2C reaction. The hardness of the melted zone was affected by both the fraction of the retained austenite and the morphology of the ledeburite eutectic.
Analyzing Uncertainty of an Ankle Joint Model with Genetic Algorithm
Authors: Ciszkiewicz Adam
Published: March 2020

Recent studies in biomechanical modeling suggest a paradigm shift, in which the parameters of biomechanical models would no longer treated as fixed values but as random variables with, often unknown, distributions. In turn, novel and efficient numerical methods will be required to handle such complicated modeling problems. The main aim of this study was to introduce and verify genetic algorithm for analyzing uncertainty in biomechanical modeling. The idea of the method was to encode two adversarial models within one decision variable vector. These structures would then be concurrently optimized with the objective being the maximization of the difference between their outputs. The approach, albeit expensive numerically, offered a general formulation of the uncertainty analysis, which did not constrain the search space. The second aim of the study was to apply the proposed procedure to analyze the uncertainty of an ankle joint model with 43 parameters and flexible links. The bounds on geometrical and material parameters of the model were set to 0.50 mm and 5.00% respectively. The results obtained from the analysis were unexpected. The two obtained adversarial structures were almost visually indistinguishable and differed up to 38.52% in their angular displacements.
Relationship between the Surface Roughness of Biodegradable Mg-Based Bulk Metallic Glass and the Osteogenetic Ability of MG63 Osteoblast-Like Cells
Authors: Wong Pei-Chun , Song Sin-Mao , Tsai Pei-Hua , Nien Yi-Yuan , Jang Jason Shian-Ching , Cheng Cheng-Kung , Chen Chih-Hwa
Published: March 2020

Mg-based bulk metallic glass materials have been investigated for their large potential for application in orthopedic implants due to their biocompatibility, low degradation rate, and osteogenetic ability. As an orthopedic implant, initial cell adhesion has been a critical issue for subsequent osteogenesis and bone formation because the first contact between cells and the implant occurs upon the implants surface. Here, we aimed to create Mg-based bulk metallic glass samples with three different surface roughness attributes in order to understand the degradation behavior of Mg-based bulk metallic glass and the adhesion ability and osteogenetic ability of the contact cells. It was found that the degradation behavior of Mg66Zn29Ca5 bulk metallic glass was not affected by surface roughness. The surface of the Mg66Zn29Ca5 bulk metallic glass samples polished via #800 grade sandpaper was found to offer a well-attached surface and to provide a good cell viability environment for Human MG63 osteoblast-like cell line. In parallel, more calcium and mineral deposition was investigated on extracellular matrix with higher surface roughness that verify the relationship between surface roughness and cell performance.
An Efficacy Assessment of Phosphate Removal from Drainage Waters by Modified Reactive Material
Authors: Grela Agnieszka , Łach Michał , Mikuła Janusz
Published: March 2020

Phosphates may pose a threat to the aquatic ecosystem when there is a connection or a path between the soil and the aquatic ecosystem. Runoff and drainage ditches connect arable land with the waters of the receiver. Phosphates in the runoff and the ditches contribute to the negative phenomenon of surface water eutrophication. In order to prevent it, certain reactive materials are used which are capable of the selective removal of compounds by way of sorption or precipitation. Zeolites can be distinguished among the many reactive materials. Within the present analysis, the modification of a reactive material containing zeolites was carried out using calcium hydroxide solutions of different concentrations. A certain concentration of calcium hydroxide was created for use in further studies. In order to characterise the new material, an analysis was done of the chemical and mineral composition, as well as the porous texture and morphology. The efficacy of phosphate removal for its typical concentrations in drainage waters in Poland was confirmed by way of an experiment. Using a modified reactive material as an element of landscape structures may reduce the negative impact of phosphates on the quality of surface water.
A Phenotarget Approach for Identifying an Alkaloid Interacting with the Tuberculosis Protein Rv1466
Authors: Xie Yan , Feng Yunjiang , Di Capua Angela , Mak Tin , Buchko Garry W. , Myler Peter J. , Liu Miaomiao , Quinn Ronald J.
Published: March 2020

In recent years, there has been a revival of interest in phenotypic-based drug discovery (PDD) due to target-based drug discovery (TDD) falling below expectations. Both PDD and TDD have their unique advantages and should be used as complementary methods in drug discovery. The PhenoTarget approach combines the strengths of the PDD and TDD approaches. Phenotypic screening is conducted initially to detect cellular active components and the hits are then screened against a panel of putative targets. This PhenoTarget protocol can be equally applied to pure compound libraries as well as natural product fractions. Here we described the use of the PhenoTarget approach to identify an anti-tuberculosis lead compound. Fractions from Polycarpa aurata were identified with activity against Mycobacterium tuberculosis H37Rv. Native magnetic resonance mass spectrometry (MRMS) against a panel of 37 proteins from Mycobacterium proteomes showed that a fraction from a 95% ethanol re-extraction specifically formed a protein-ligand complex with Rv1466, a putative uncharacterized Mycobacterium tuberculosis protein. The natural product responsible was isolated and characterized to be polycarpine. The molecular weight of the ligand bound to Rv1466, 233 Da, was half the molecular weight of polycarpine less one proton, indicating that polycarpine formed a covalent bond with Rv1466.
An Integrated Multi-Disciplinary Perspective for Addressing Challenges of the Human Gut Microbiome
Authors: Shah Rohan M. , McKenzie Elizabeth J. , Rosin Magda T. , Jadhav Snehal R. , Gondalia Shakuntla V. , Rosendale Douglas , Beale David J.
Published: March 2020

Our understanding of the human gut microbiome has grown exponentially. Advances in genome sequencing technologies and metagenomics analysis have enabled researchers to study microbial communities and their potential function within the context of a range of human gut related diseases and disorders. However, up until recently, much of this research has focused on characterizing the gut microbiological community structure and understanding its potential through system wide (meta) genomic and transcriptomic-based studies. Thus far, the functional output of these microbiomes, in terms of protein and metabolite expression, and within the broader context of host-gut microbiome interactions, has been limited. Furthermore, these studies highlight our need to address the issues of individual variation, and of samples as proxies. Here we provide a perspective review of the recent literature that focuses on the challenges of exploring the human gut microbiome, with a strong focus on an integrated perspective applied to these themes. In doing so, we contextualize the experimental and technical challenges of undertaking such studies and provide a framework for capitalizing on the breadth of insight such approaches afford. An integrated perspective of the human gut microbiome and the linkages to human health will pave the way forward for delivering against the objectives of precision medicine, which is targeted to specific individuals and addresses the issues and mechanisms in situ.
High-Sensitivity Detection of IgG Operating near the Dispersion Turning Point in Tapered Two-Mode Fibers
Authors: Sun Bing , Wang Yiping
Published: March 2020

The conventional methods for monitoring IgG levels suffer from some apparent problems such as long assay time, multistep processing, and high overall cost. An effective and suitable optical platform for label-free biosensing was investigated by the implementation of antibody/antigen immunoassays. The ultrasensitive detection of IgG levels could be achieved by exploiting the dispersion turning point (DTP) existing in the tapered two-mode fibers (TTMFs) because the sensitivity will reach ±∞ on either side of the DTP. Tracking the resonant wavelength shift, it was found that the fabricated TTMF device exhibited limits of detection (LOD) down to concentrations of 10 fg/mL of IgG in PBS solution. Such immunosensors based on DTP have great significance on trace detection of IgG due to simple detection scheme, quick response time, and miniaturization.
Engineering Microneedles for Therapy and Diagnosis: A Survey
Authors: Xie Liping , Zeng Hedele , Sun Jianjun , Qian Wei
Published: March 2020

Microneedle (MN) technology is a rising star in the point-of-care (POC) field, which has gained increasing attention from scientists and clinics. MN-based POC devices show great potential for detecting various analytes of clinical interests and transdermal drug delivery in a minimally invasive manner owing to MNs’ micro-size sharp tips and ease of use. This review aims to go through the recent achievements in MN-based devices by investigating the selection of materials, fabrication techniques, classification, and application, respectively. We further highlight critical aspects of MN platforms for transdermal biofluids extraction, diagnosis, and drug delivery assisted disease therapy. Moreover, multifunctional MNs for stimulus-responsive drug delivery systems were discussed, which show incredible potential for accurate and efficient disease treatment in dynamic environments for a long period of time. In addition, we also discuss the remaining challenges and emerging trend of MN-based POC devices from the bench to the bedside.
Ultrasonic-Assisted Extraction, Structural Characterization, Chain Conformation, and Biological Activities of a Pectic-Polysaccharide from Okra (Abelmoschus esculentus)
Authors: Nie Xi-Rui , Fu Yuan , Wu Ding-Tao , Huang Ting-Ting , Jiang Qin , Zhao Li , Zhang Qing , Lin De-Rong , Chen Hong , Qin Wen
Published: March 2020

The purpose of this study was to better understand the chemical characteristics and chain conformation of okra polysaccharides extracted by ultrasonic-assisted extraction. A pectic-polysaccharide, named OPP-D, was obtained, which was mainly composed of rhamnose, galacturonic acid, and galactose with a molar ratio of 1.01:1.00:2.31. Combined with NMR analysis, -4)-α-d-GalAp-(1,2,4)-α-l-Rhap-(1- were identified as the backbone with galactan side chains substituted partly at O-4 of Rhap. Molecular weight and radius of gyration of OPP-D were determined as 2.19 × 105 Da and 27.0 nm, respectively. OPP-D was determined as an air-core sphere with branching chains in 0.9% NaCl solution by high-performance size-exclusion chromatography coupled with multi-angle laser light scattering and dynamic light scattering for the first time. Moreover, OPP-D exhibited typical shear-thinning behavior. In addition, OPP-D exhibited remarkable in vitro antioxidant activities and prebiotic activities, while the relatively high molecular weight, high degree of esterification, high content of uronic acids, and highly branched globular conformation of OPP-D might contribute to its in vitro anti-diabetic activities and binding capacities. Results can contribute to a better understanding of the structure–bioactivity relationship of OPPs, and OPP-D has great potential applications in the functional food and pharmaceutical industries.
Fully Biobased Epoxy Resins from Fatty Acids and Lignin
Authors: Ortiz Pablo , Vendamme Richard , Eevers Walter
Published: March 2020

The use of renewable resources for plastic production is an imperious need for the reduction of the carbon footprint and the transition towards a circular economy. With that goal in mind, fully biobased epoxy resins have been designed and prepared by combining epoxidized linseed oil, lignin, and a biobased diamine derived from fatty acid dimers. The aromatic structures in lignin provide hardness and strength to an otherwise flexible and breakable epoxy resin. The curing of the system was investigated by infrared spectroscopy and differential scanning calorimetry (DSC). The influence of the different components on the thermo-mechanical properties of the epoxy resins was analyzed by DSC, thermal gravimetric analysis (TGA), and tensile tests. As the content of lignin in the resin increases, so does the glass transition, the Young’s modulus, and the onset of thermal degradation. This correlation is non-linear, and the higher the percentage of lignin, the more pronounced the effect. All the components of the epoxy resin being commodity chemicals, the present system provides a realistic opportunity for the preparation of fully biorenewable resins at an industrial scale.
Interaction of Coumarin Phytoestrogens with ERα and ERβ: A Molecular Dynamics Simulation Study
Authors: Wang Ting , Wang Yunfei , Zhuang Xuming , Luan Feng , Zhao Chunyan , Cordeiro M. Natália D. S.
Published: March 2020

Coumarin phytoestrogens, as one of the important classes of phytoestrogens, have been proved to play an important role in various fields of human life. In this study, molecular simulation method including molecular docking and molecular dynamics methods were performed to explore the various effects between four classical coumarin phytoestrogens (coumestrol, 4-methoxycoumestrol, psoralen and isopsoralen), and estrogen receptors (ERα, ERβ), respectively. The calculated results not only proved that the four coumarin phytoestrogens have weaker affinity than 17β-estradiol to both ERα, and ERβ, but also pointed out that the selective affinity for ERβ is greater than ERα. In addition, the binding mode indicated that the formation of hydrogen bond and hydrophobic interaction have an important effect on the stability of the complexes. Further, the calculation and decomposition of binding free energy explored the main contribution interactions to the total free energy.
Chemical Characterization of Capsule-Brewed Espresso Coffee Aroma from the Most Widespread Italian Brands by HS-SPME/GC-MS
Authors: Lolli Veronica , Acharjee Animesh , Angelino Donato , Tassotti Michele , Del Rio Daniele , Mena Pedro , Caligiani Augusta
Published: March 2020

Coffee capsules market is on the rise as it allows access to a wide selection of coffee, differing in taste and brand. However, few data about the chemical characterization of the capsule-brewed coffee aroma are available. In this work, an untargeted approach using headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography–mass spectrometry (GC-MS) and combined to chemometrics was performed to study and compare aroma profile from 65 capsule-brewed espresso coffees (ECs) commercialized by five of the most representative brands in Italy. Volatile profiles obtained from ECs were subjected to multivariate statistical analysis, which generally did not show a significant variability among coffees belonging to the same brand, except for those modified after the addition of specific flavor additives or aromatic substances (such as caramel, chocolate, etc.). Similarities may be related to the starting coffee brew or the processing method, which is likely the same for each individual brand. Additionally, partial least squares discriminant analysis (PLS-DA) showed that capsules from a specific brand contain the highest concentration of pyrazines, thus characterized by an intense and characteristic aroma, and a stronger note than those from the other brands. This study supports that the chemical analysis in conjunction with chemometric tools is a useful approach for assessing flavor quality, even if the need remains to identify volatile markers of high-quality beverages.
In Vitro and In Vivo Effects of Flavonoids on Peripheral Neuropathic Pain
Authors: Basu Paramita , Basu Arpita
Published: March 2020

Neuropathic pain is a common symptom and is associated with an impaired quality of life. It is caused by the lesion or disease of the somatosensory system. Neuropathic pain syndromes can be subdivided into two categories: central and peripheral neuropathic pain. The present review highlights the peripheral neuropathic models, including spared nerve injury, spinal nerve ligation, partial sciatic nerve injury, diabetes-induced neuropathy, chemotherapy-induced neuropathy, chronic constriction injury, and related conditions. The drugs which are currently used to attenuate peripheral neuropathy, such as antidepressants, anticonvulsants, baclofen, and clonidine, are associated with adverse side effects. These negative side effects necessitate the investigation of alternative therapeutics for treating neuropathic pain conditions. Flavonoids have been reported to alleviate neuropathic pain in murine models. The present review elucidates that several flavonoids attenuate different peripheral neuropathic pain conditions at behavioral, electrophysiological, biochemical and molecular biological levels in different murine models. Therefore, the flavonoids hold future promise and can be effectively used in treating or mitigating peripheral neuropathic conditions. Thus, future studies should focus on the structure-activity relationships among different categories of flavonoids and develop therapeutic products that enhance their antineuropathic effects.