bluuebunny/crossref_metadata_2025 huggingface dataset:
Side-to-side differences in knee laxity and side hop test may predispose an anterior cruciate ligament reinjury in competitive adolescent alpine skiers
Authors: Westin Maria , Mirbach Lisbeth I. , Harringe Marita L.
Published: October 2022

An anterior cruciate ligament (ACL) injury is a common, severe injury in alpine skiing, and anterior cruciate ligament reconstruction (ACLR) is frequently performed in competitive alpine skiers younger than 20 years old. To reduce the reinjury rate, both intrinsic and extrinsic risk factors should be examined. The aim of this study was to investigate possible intrinsic risk factors for an ACL reinjury in competitive alpine skiers. A cohort of 384 alpine skiers (191 males/193 females) from the Swedish ski high schools were prospectively followed during their high school years. The students were clinically examined and physically tested prior to each ski season. In addition, the RAND 36-Item health survey 1.0 (SF-36, Copyright 1994 Medical Outcome Trust, distributed by RAND Corporation) and injuries were prospectively registered. Thirty-one of the skiers (five males/26 females) had undergone an ACLR before entering the ski high school. This cohort was analyzed with respect to the occurrence of, and possible risk factors for an ACL reinjury (including ipsilateral and contralateral ACL injuries). Skiers who sustained an ACL reinjury were called the “ACL reinjury group,” and those who did not sustain an ACL reinjury were called the “ACL injury group.” Notably, 12 of the 31 students (39%), ten female and two male skiers, aged 16.5 (SD 0.5) years, sustained an ACL reinjury during the two first years at the ski high school. In addition, 10 of the 12 ACL reinjuries occurred within 10–23 months from the first injury [m 14.8 (SD4.7)] and two ACL reinjuries occurred at 29 and 47 months, respectively, from the first injury. It is noted that eight of the ACL reinjuries were to the ipsilateral knee and four to the contralateral knee. There were no differences between the groups with respect to muscle flexibility in the lower extremity, Beighton score, and one leg hop for distance or square hop test. Side-to-side differences were found with respect to knee joint laxity, >3 mm, measured with KT-1000 arthrometer (p = 0.02), and the side hop test (p = 0.04). RAND 36-Item health survey did not predict an ACL reinjury. In conclusion, a side-to-side difference in the side hop test and knee joint laxity (KT-1000) may predispose an ACL reinjury in competitive adolescent alpine skiers.
A new automatic stitching method for full-length lower limb radiography
Authors: Alkhatatbeh Tariq , Wang Jia Lin , Zhang Wei Jia , Li Yong Wei , Xia Yong , Wang Wei
Published: October 2022

Full-length lower limb x-rays are used to diagnose and plan surgical procedures, such as Total Knee Arthroplasty (TKA) and High Tibial Osteotomy (HTO). Due to the size limitation of digital radiography (DR), panoramic x-ray images cannot be obtained in a single exposure, necessitating multiple exposures and image stitching. In favor of manually constructing full-length x-ray images, we propose a new feature-based automated method for stitching together x-ray images. This new method is based on Canny algorithm, which detects and aligns bone edges before fusing them using a Wavelet form domain. Twenty-eight sets of lower limb x-ray images obtained from our hospital have been stitched and evaluated. The hip, knee, and ankle (HKA) angle was computed in two different ways then compared to manually stitched x-ray images by an expert. The stitching time was only three seconds, and the P-value was P = 0.974, and an accuracy rate of 100% was found. This method demonstrated greater precision and speed than both manually stitched x-ray images and previously published methods.
MatricS—A novel tool for monitoring professional role development in surgical disciplines
Authors: Necknig U. , Leyh H. , Waidelich R. , Gernhold L. , Kiesewetter J. , Weidenbusch M.
Published: October 2022

Introduction

Mentoring is an effective method for human resource development. Monitoring the process is important for individual mentee/mentor pairs as well as for program directors. Due to individual personality differences of both mentees and mentors and their respective interactions, it is challenging to monitor the individual development process of mentees in a structured manner. This study investigates to what extent a novel instrument, the mentee-based assessment tool for role development of interpersonal competencies in surgical professions (MatricS) can adequately monitor the professional role development process of residents during an established mentoring program.

Material and methods

In a prospective longitudinal study, the competence development of 31 mentees in two subsequent cohorts was assessed by a modified role matrix based on Canadian Medical Education Directives for Specialists. The evaluation focused on three defined roles (D, developer; N, networker; M, multiplicator) at three levels (private, employer-related, national/international) with four stages of development. For validation of mentee self-assessments, the assessments of the respective mentors were recorded alongside. For correlation analyses, Pearson coefficients were calculated, pre-post-comparisons were done by paired t-tests; significance was assumed at p < 0.05, respectively.

Results

Mentee self-assessments overall correlated well with the objective mentor assessments (Pearson's r 0.8, p < 0.001). Significant correlations of this magnitude were found for both individual cohorts as well as for all individual roles. The mentees acquired competencies in all roles indicated by significant increases of corresponding MatricS scores. The largest competency gains (mean ± SD) were found in the role D (start: 1.30 ± 0.77, end: 2.13 ± 0.83, p < 0.001). The majority of mentees achieved the prespecified target competency level in >75% of all roles and levels.

Conclusion

The role development process during mentoring can be reliably monitored by using MatricS. MatricS scores highly correlate between mentees and mentors, indicating that mentee self-assessments are suitable and sufficient for monitoring. These findings help to lessen the work burden on senior surgeons and thus can help to increase the acceptance of mentoring programs in surgical disciplines.
Three-dimensional printing and 3D slicer powerful tools in understanding and treating neurosurgical diseases
Authors: You Yijie , Niu Yunlian , Sun Fengbing , Huang Sheng , Ding Peiyuan , Wang Xuhui , Zhang Xin , Zhang Jian
Published: October 2022

With the development of the 3D printing industry, clinicians can research 3D printing in preoperative planning, individualized implantable materials manufacturing, and biomedical tissue modeling. Although the increased applications of 3D printing in many surgical disciplines, numerous doctors do not have the specialized range of abilities to utilize this exciting and valuable innovation. Additionally, as the applications of 3D printing technology have increased within the medical field, so have the number of printable materials and 3D printers. Therefore, clinicians need to stay up-to-date on this emerging technology for benefit. However, 3D printing technology relies heavily on 3D design. 3D Slicer can transform medical images into digital models to prepare for 3D printing. Due to most doctors lacking the technical skills to use 3D design and modeling software, we introduced the 3D Slicer to solve this problem. Our goal is to review the history of 3D printing and medical applications in this review. In addition, we summarized 3D Slicer technologies in neurosurgery. We hope this article will enable many clinicians to leverage the power of 3D printing and 3D Slicer.
Incidence of ischemic complications and technical nuances of arteries preservation for insular gliomas resection
Authors: Hou Zonggang , Huang Zhenxing , Li Zhenye , Deng Zhenghai , Li Gen , Xu Yaokai , Wang Mingran , Sun Shengjun , Zhang Yazhuo , Qiao Hui , Xie Jian
Published: October 2022

Introduction

Insular gliomas have complex anatomy and microvascular supply that make resection difficult. Furthermore, resection of insular glioma is associated with a significant risk of postoperative ischemic complications. Thus, this study aimed to assess the incidence of ischemic complications related to insular glioma resection, determine its risk factors, and describe a single surgeon’s experience of artery-preserving tumor resection.

Methods

We enrolled 75 consecutive patients with insular gliomas who underwent transcortical tumor resection. Preoperative and postoperative demographic, clinical, radiological [including diffusion-weighted imaging (DWI)], intraoperative neurophysiological data, and functional outcomes were analyzed. Motor evoked potentials (MEPs) and radiological characteristics like the relationship between the proximal segment of the lateral lenticulostriate arteries (LLSAs) and the tumor, the flat inner edge sign (the inner edge of the insular glioma is well-defined) or obscure inner edge sign, the distance between the lesion and posterior limb of the internal capsule and the invasion of the superior limiting sulcus by the tumor were analyzed. Strategies such as “residual triangle,” “basal ganglia outline reappearance,” and “sculpting” technique were used to preserve the LLSAs and the main branches of M2 for maximal tumor resection according to the Berger–Sinai classification.

Results

Postoperative DWI showed acute ischemia in 44 patients (58.7%). Moreover, nine patients (12%) had developed new motor deficits, as determined by the treating neurosurgeons. The flat inner edge sign [odds ratio (OR), 0.144; 95% confidence interval (CI), 0.024–0.876) and MEPs (>50%) (OR, 18.182; 95% CI, 3.311–100.00) were significantly associated with postoperative core ischemia, which affected the posterior limb of the internal capsule or corona radiata.

Conclusions

Insular glioma resection was associated with a high incidence of ischemia, as detected by DWI, as well as new motor deficits that were determined by the treating neurosurgeons. Insular glioma patients with obscure inner edge signs and intraoperative MEPs decline >50% had a higher risk of developing core ischemia. With our strategies, maximal safe resection of insular gliomas may be achieved.
Comparative study of pelvic sarcoma patients undergoing internal and external hemipelvectomy: A meta-analysis study
Authors: Banskota Nishant , Yang Hongsheng , Fang Xiang , Yuan Dechao , Zhang Wenli , Duan Hong
Published: October 2022

Introduction

Malignant and giant pelvic tumors are complex and rare, and hemipelvectomies are complex procedures performed for this malignant lesion. Only a few studies had been conducted on the survival and recurrence of pelvic sarcomas patients undergoing internal or external hemipelvectomy. In the present study, we compared internal with external hemipelvectomy in pelvic sarcomas on clinical outcomes by a meta-analysis.

Methods

The survival and recurrence rates of pelvic sarcomas patients were collected from research reports from CNKI, MEDLINE, EMBASE, the Cochrane Database, and Google Scholar until April 2022. The quality of included articles was evaluated by two independent reviewers. Differences between patients undergoing internal and external hemipelvectomy were analyzed based on postoperative survival and recurrence rates.

Results

Five articles were included according to selection criteria. There were 183 patients in total from these studies. Our results showed that there was no significant difference between limb salvage surgery and amputation according to survival; however, patients with internal hemipelvectomy had a lower recurrence rate.

Conclusions

Internal hemipelvectomy results in a lower recurrence rate and similar survival rate, while not increasing the risk of metastasis and complications. This study provided more pieces of evidence to support internal hemipelvectomy as a favorable treatment of pelvic sarcomas.
A risk prediction model for evaluating thrombosis extension of muscle calf venous thrombosis after craniotomy
Authors: Li Juhua , Chen Huayu , Liu Mei , Lin Zheng , Ren Xingzhen , Wang Ying , Zou Xingchen , Gu Zejuan
Published: October 2022

Objective

To explore the risk factors of muscle calf venous thrombosis (MCVT) after craniotomy and construct a risk prediction model, so as to provide tool for evaluating the prognosis of MCVT after craniotomy.

Methods

Retrospective analysis was performed on the data of patients undergoing craniotomy complicated with MCVT from January 1, 2018 to December 31, 2020. A prediction model was established by Logistic regression, and the predictive efficacy of the model was tested by ROC curve. The accuracy of the risk model was evaluated by Hosmer-Lemeshow (H-L) test, and the model was verified internally by cross validation.

Results

Among the 446 patients who underwent craniotomy complicated with MCVT, 112 cases (25.11%) had thrombosis extension. D-dimer, Capirini scores, length of hospital stay, malignant tumor, fracture, use of dehydrating agents and hemostatic agents were independently related to thrombosis extension after craniotomy. The area under ROC curve (AUROC) of the prediction model was 0.918 (0.888, 0.942), and the sensitivity and specificity of the maximum Youden index were 85.3% and 78.2%, respectively. H-L test showed that the prediction model was accurate (χ2 = 12.426, P = 0.133). The internal verification results of the prediction model showed that the AUROC value of the prediction model is 0.892.

Conclusion

The prediction model has a good prediction efficacy on the prognosis of post-craniotomy patients complicated with MCVT, and can be used as a tool to evaluate the risk of thrombosis extension.
Laparoendoscopic single-site surgery for adnexal disease during pregnancy: A single-center preliminary experience
Authors: Yin Min , Yang Jiaxin , Zhou Huimei , Zhang Xinyue
Published: October 2022

Purpose

This study aimed to evaluate the safety and efficacy of laparoendoscopic single-site surgery (LESS) in treating adnexal disease during pregnancy.

Methods

Medical records of included patients were retrospectively reviewed and follow-ups of all the patients were performed until the delivery of the fetus. The clinical characteristics, surgical interventions, postoperative complications, and pregnancy outcomes were analyzed.

Results

Six cases were included, with the gestational age ranging from 19 to 31 weeks 1 day. Procedures included salpingo-oophorectomy (n = 3), ovarian or paratubal cystectomy with detorsion (n = 2), and adnexal detorsion (n = 1). The median duration of surgery was 35 min (range, 20–60 min), and the estimated blood loss ranged from 5 to 50 ml. No major intraoperative or postoperative complications were noted. The final pathologic results included high-grade serous ovarian carcinoma, ovarian borderline serous cystadenoma, ovarian simple cyst, endometrioma, and mesosalpinx cyst. Five patients had a spontaneous vaginal delivery at full-term, and one patient had a cesarean section preterm followed by comprehensive staging surgery of ovarian cancer.

Conclusion

Based on the data we included, LESS performed by experienced surgeons appeared to be a safe and feasible alternative to multiport laparoscopic surgery for the management of selected patients with adnexal disease during pregnancy. More studies with large sample sizes at multiple centers are warranted.
Dairy sheep and goats prefer the single components over the mixed ration
Authors: Berthel Roxanne , Simmler Michael , Dohme-Meier Frigga , Keil Nina
Published: October 2022

Mixed rations provide ruminants with a balanced diet by aiming to prevent selective feeding. However, this is a natural behavior of sheep and goats based on their dietary needs and the nutritional properties of feedstuffs. Therefore, the present study investigates non-lactating dairy sheep's and goats' acceptance of a mixed ration when it is offered as choice next to its single components. Because all offered feeds were of comparable nutritional value, the animals were expected to not show a particular preference. Twelve pairs of sheep and goats each, were offered three different feeds simultaneously for 5 replicate days. Two feeds consisted of a single component, hay (H) or grass-silage (G) of similar nutritional value. The third feed was a mixed ration (M) including both single-feed components in a 50:50 dry matter (DM) ratio. Feeds were offered ad libitum twice daily. The animals' intake of each feed was recorded at six time points per day by weighing the leftovers. Feed preference was expressed as the natural logarithm of the ratio of the intake of the single component to the intake of M and analyzed using linear mixed-effects models. Additionally, the animals' first choices after gaining access to the feeds were recorded at each weighing event and analyzed using an item response tree generalized mixed-effects model. The sheep's average daily DM intake was 59 (±11)% G, 26 (±10)% H, and 15 (±10)% M (mean ± standard deviation). Goats consumed an average of 56 (±13)% G, 37 (±12)% H, and 7 (±6)% M daily. Both species preferred the single components to M in all observation periods. The proportions of the three feeds consumed differed throughout the day and between species. For both species, the estimated probability that an animal chooses a single component over M first was over 94% at all time points. These results show that, contrary to our expectations, non-lactating dairy sheep and goats prefer single components over a mixed ration of the same components and similar nutritional value. This might be caused by the animals seeking to diversify their feed throughout the day independent of apparent nutritional values and/or because sensory properties of the single components, indicating palatability, are relevantly reduced by mixing.
Bioequivalence of two tablet formulations of cefpodoxime proxetil in beagle dogs
Authors: Gao Yan-Yan , Sang Ka-Na , Li Peng-Peng , Hao Jie , Zhang Cong , Li Huan-Juan , Zhou De-Gang
Published: October 2022

The pharmacokinetic profiles and bioequivalence of two cefpodoxime proxetil tablets were investigated in Beagle dogs. A single-dose, four-way complete replication and crossover design was used in the present study. A total of 28 healthy Beagle dogs (half male and female) with an average body weight of 11.1 kg were randomly allocated to this study. A whole reference or test tablet containing the equivalent of 100 mg of cefpodoxime was administered orally to each dog. Serial plasma samples were collected, and cefpodoxime concentrations were determined by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Then a non-compartmental method was used to calculate the pharmacokinetic parameters of both tablet formulations. The average bioequivalence (ABE) or reference-scaled average bioequivalence (RSABE) methods were used to determine the 90% confidence interval (CI) of AUCINF_obs and Cmax. No significant differences were observed for both parameters between both tablets. The test formulation was bioequivalent to the reference one because the 90% CI ranges of Cmax and AUCINF_obs were all between 80 and 125%.
Impact of Iterative Bilateral Filtering on the Noise Power Spectrum of Computed Tomography Images
Authors: Anam Choirul , Naufal Ariij , Sutanto Heri , Adi Kusworo , Dougherty Geoff
Published: October 2022

A bilateral filter is a non-linear denoising algorithm that can reduce noise while preserving the edges. This study explores the characteristics of a bilateral filter in changing the noise and texture within computed tomography (CT) images in an iterative implementation. We collected images of a homogeneous Neusoft phantom scanned with tube currents of 77, 154, and 231 mAs. The images for each tube current were filtered five times with a configuration of sigma space (σd) = 2 pixels, sigma intensity (σr) = noise level, and a kernel of 5 × 5 pixels. To observe the noise texture in each filter iteration, the noise power spectrum (NPS) was obtained for the five slices of each dataset and averaged to generate a stable curve. The modulation-transfer function (MTF) was also measured from the original and the filtered images. Tests on an anthropomorphic phantom image were carried out to observe their impact on clinical scenarios. Noise measurements and visual observations of edge sharpness were performed on this image. Our results showed that the bilateral filter was effective in suppressing noise at high frequencies, which is confirmed by the sloping NPS curve for different tube currents. The peak frequency was shifted from about 0.2 to about 0.1 mm−1 for all tube currents, and the noise magnitude was reduced by more than 50% compared to the original images. The spatial resolution does not change with the number of iterations of the filter, which is confirmed by the constant values of MTF50 and MTF10. The test results on the anthropomorphic phantom image show a similar pattern, with noise reduced by up to 60% and object edges remaining sharp.
Structural Model Based on Genetic Algorithm for Inhibiting Fatty Acid Amide Hydrolase
Authors: Trif Cosmin , Mihai Dragos Paul , Zanfirescu Anca , Nitulescu George Mihai
Published: October 2022

The fatty acid amide hydrolase (FAAH) is an enzyme responsible for the degradation of anandamide, an endocannabinoid. Pharmacologically blocking this target can lead to anxiolytic effects; therefore, new inhibitors can improve therapy in this field. In order to speed up the process of drug discovery, various in silico methods can be used, such as molecular docking, quantitative structure–activity relationship models (QSAR), and artificial intelligence (AI) classification algorithms. Besides architecture, one important factor for an AI model with high accuracy is the dataset quality. This issue can be solved by a genetic algorithm that can select optimal features for the prediction. The objective of the current study is to use this feature selection method in order to identify the most relevant molecular descriptors that can be used as independent variables, thus improving the efficacy of AI algorithms that can predict FAAH inhibitors. The model that used features chosen by the genetic algorithm had better accuracy than the model that used all molecular descriptors generated by the CDK descriptor calculator 1.4.6 software. Hence, carefully selecting the input data used by AI classification algorithms by using a GA is a promising strategy in drug development.
Antileishmanial Activity of 4,8-Dimethoxynaphthalenyl Chalcones on Leishmania amazonensis
Authors: de Santiago-Silva Kaio Maciel , Bortoleti Bruna Taciane da Silva , Oliveira Laudicéa do Nascimento , Maia Fernanda Lima de Azevedo , Castro Joyce Cristina , Costa Ivete Conchon , Lazarin Danielle Bidóia , Wardell James L. , Wardell Solange M. S. V. , Albuquerque Magaly Girão , Lima Camilo Henrique da Silva , Pavanelli Wander Rogério , Bispo Marcelle de Lima Ferreira , Gonçalves Raoni Schroeder B.
Published: October 2022

Leishmaniasis is a neglected tropical disease caused by Leishmania species. Available therapeutic options have several limitations. The drive to develop new, more potent, and selective antileishmanial agents is thus a major goal. Herein we report the synthesis and the biological activity evaluation against promastigote and amastigote forms of Leishmania amazonensis of nine 4,8-dimethoxynaphthalenyl chalcones. Compound ((E)-1-(4,8-dimethoxynaphthalen-1-yl)-3-(4-nitrophenyl)prop-2-en-1-one), 4f, was the most promising with an IC50 = 3.3 ± 0.34 μM (promastigotes), a low cytotoxicity profile (CC50 = 372.9 ± 0.04 μM), and a high selectivity index (SI = 112.6). Furthermore, 4f induced several morphological and ultrastructural changes in the free promastigote forms, loss of plasma membrane integrity, and increased reactive oxygen species (ROS). An in silico analysis of drug-likeness and ADME parameters suggested high oral bioavailability and intestinal absorption. Compound 4f reduced the number of infected macrophages and the number of amastigotes per macrophage, with an IC50 value of 18.5 ± 1.19 μM. Molecular docking studies with targets, ARG and TR, showed that compound 4f had more hydrogen bond interactions with the ARG enzyme, indicating a more stable protein-ligand binding. These results suggest that 4,8-dimethoxynaphthalenyl chalcones are worthy of further study as potential antileishmanial drugs.
Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves
Authors: Goulart Debora Brito , Beyi Ashenafi Feyisa , Wu Zuowei , Adiguzel Mehmet Cemal , Wilson Samantha , Xu Changyun , Pang Jinji , Dewell Renee , Dewell Grant A. , Plummer Paul J. , Zhang Qijing , Sahin Orhan
Published: October 2022

Fluoroquinolone (FQ) resistance in a major foodborne bacterial pathogen, Campylobacter jejuni, derived from cattle has recently become prevalent and poses a significant public health concern. However, the underlying factors for this increase are not entirely clear. To evaluate the effect of enrofloxacin treatment on FQ-resistance development in C. jejuni, 35 commercial calves were equally divided into five groups (Groups 1–5) and were orally inoculated with FQ-susceptible (FQ-S) C. jejuni. Eight days later, Groups 4 and 5 were challenged with Mannheimia haemolytica via a transtracheal route to induce a respiratory disease; after 8 days, Groups 2, 3, 4, and 5 were injected subcutaneously with enrofloxacin (7.5 mg/kg for Groups 2 and 4, and 12.5 mg/kg for Groups 3 and 5). Colonization levels by FQ-resistant (FQ-R) and FQ-S Campylobacter in rectal feces were determined via differential culture throughout the experiment. Before oral inoculation with C. jejuni, only five calves were naturally colonized by Campylobacter, four of which were also colonized by FQ-R C. jejuni (three in Group 1 and one in Group 3). Soon after the oral inoculation, almost all calves in the groups became stably colonized by FQ-S C. jejuni (~3–6 log10 CFU/g), except that the four calves that were pre-colonized before inoculation remained positive with both FQ-R and FQ-S C. jejuni. Following enrofloxacin administration, C. jejuni colonization declined sharply and rapidly in all treated groups to undetectable levels; however, the vast majority of the animals were recolonized by C. jejuni at comparable levels 72 h after the treatment. Notably, no FQ-R C. jejuni was detected in any of the calves that received enrofloxacin, regardless of the drug dose used or disease status of the animals. The lack of detection of FQ-R C. jejuni was likely due to the localized high concentration of the antibiotic in the intestine, which may have prevented the emergence of the FQ-R mutant. These findings indicate that single-dose enrofloxacin use in cattle poses a low risk for selection of de novo FQ-R mutants in C. jejuni.
Reimplantation after Periprosthetic Joint Infection: The Role of Microbiology
Authors: Suardi Virginia , Logoluso Nicola , Anghilieri Filippo Maria , Santoro Giuseppe , Pellegrini Antonio Virgilio
Published: October 2022

Periprosthetic joint infection (PJI) is among the most feared orthopedic complications. Critical questions are whether the infection is completely resolved before reimplantation and what the clinical significance of positive culture is at reimplantation. The aim of this study was to determine whether a correlation exits between culture results at reimplantation after spacer insertion for hip and knee PJI and treatment failure rate. The data of 84 patients who underwent two-stage exchange arthroplasty for hip or knee PJI were reviewed and the results of intraoperative culture at reimplantation were analyzed quantitatively and qualitatively. Correlations were sought between these patterns and treatment outcome. Our data indicate no evidence for a correlation between positive culture at reimplantation and greater risk of treatment failure. Nonetheless, we noted a higher, albeit statistically not significant rate of treatment failure in patients with at least two samples testing positive. The role of microbiology at reimplantation remains unclear, but a positive culture might signal increased risk for subsequent implant failure. Further studies are needed to elucidate the implications of this finding.
Milk Thistle Oilseed Cake Flour Fractions: A Source of Silymarin and Macronutrients for Gluten-Free Bread
Authors: Bedrníček Jan , Lorenc František , Jarošová Markéta , Bártová Veronika , Smetana Pavel , Kadlec Jaromír , Jirotková Dana , Kyselka Jan , Petrášková Eva , Bjelková Marie , Konvalina Petr , Hoang Trong Nghia , Bárta Jan
Published: October 2022

The utilization of plant by-products as functional food ingredients has received increasing attention in the last decade. One such by-product generated during milk thistle oil pressing is oilseed cakes, which could be used as a novel food ingredient. Therefore, the study aimed at investigating the effects of the addition of milk thistle oilseed cake (MTOC) flour fractions obtained via dry sieving, differing in particle size (unsieved; coarse: >710 µm; medium: 315–710 µm; and fine: <315 µm), on the quality of gluten-free bread and stability of silymarin during breadmaking. The 10% addition of the fractions into gluten-free bread increased the protein, fibre, fat, ash and silymarin content. The breads with the coarse fraction had the highest content of fibre, whereas the breads with the fine fraction excelled in protein, fat and ash content. The medium fraction was characterized as the richest source of silymarin, whilst the fine fraction was the poorest. Silymarin constituents were slightly released during dough rising but also partially decomposed during baking; moreover, silydianin was the most susceptible and degraded the most. The enriched breads had better sensory and textural properties compared to the control bread. The results suggest that MTOC flour fractions can improve the potential health benefits and nutritional profile of gluten-free bread.
Fully Nonlinear Small Amplitude Dynamical Waves for Multicomponent Complex Plasma with Kappa Distributed Electrons and Ions
Authors: Mahmoud Abeer A.
Published: October 2022

Multicomponent plasmas are ubiquitous in astrophysics and space plasma environments. In the present manuscript, we assumed a dusty plasma system consisting of negative and positive dust species and kappa-distributed electrons and ions. The analysis is based on the fluid model of plasmas, and the reductive perturbation method was used to study the behavior of propagating waves. New arbitrary parameters were obtained to measure the strength of nonlinearity, dispersion, and dissipation in the plasma system. We investigated the effects of the arbitrary parameters on the appearance of the different nonlinear waves as soliton, shock, and solitary waves. Furthermore, we studied the effects of the kappa parameter, the viscosity of dust species, and the ratio between the temperature of positive and negative dust species in the type of wave, i.e., compressive or rarefactive.
Effects of Cold Plasma Treatment on Physical Modification and Endogenous Hormone Regulation in Enhancing Seed Germination and Radicle Growth of Mung Bean
Authors: Le Thi Quynh Xuan , Nguyen Linh Nhat , Nguyen Thanh Tung , Choi Eun Ha , Nguyen Quang Liem , Kaushik Nagendra Kumar , Dao Nguyen Thuan
Published: October 2022

This study investigated the effects of plasma duration and different reactive species ratios of cold atmospheric-pressure plasma treatment on both physical and endogenous hormone changes in enhancing the germination and growth of mung bean seeds. Seed germination and sprout stem length were significantly enhanced after plasma treatment. The germination rate increased eleven times after 12 h, while the radicles’ length increased ~3 times after 96 h with optimal plasma treatment parameters. SEM images showed that the plasmas directly induced gradual changes in the seed coating, including deformed and shrunken epidermis, and cracks with sizes varying from 0.2 to 1.5 µm after 4 min of plasma treatment. Water contact angle was reduced from 73° with untreated seed to almost 0° with 4 min treated seed. These effects could lead to better water absorption on the surface of treated seeds. We found that a plasma energy dosage of 0.08 Wh per seed and NO concentration between 20–95 ppm were the optimal enhancement conditions. We also showed that, for the first time, through delicate extraction, separation, and quantification processes, NO-induced upregulation of the natural growth hormone gibberellic acid could be the dominant phytochemistry pathway responsible for the enhancement effect.
Experimental Investigation of the Fatigue Life of a Bridge Crane Girder Using S-N Method
Authors: Pástor Miroslav , Lengvarský Pavol , Hagara Martin , Kuľka Jozef
Published: October 2022

Experimental measurement methods used in operational mode provide valuable information about the behavior of mechanical parts of equipment that cannot be determined in advance by analytical calculations or numerical modeling. The strain gauge method, which is often used to investigate the stresses in the load-bearing members of steel structures under operational conditions, was used. The advantage is the fast and accurate acquisition of stress values at critical locations selected based on analytical computations or numerical modeling. In the present paper, the residual operating life of two main girders of a bridge crane was assessed by an analytical-experimental approach. The input parameters for the assessment were obtained from the evaluated stress time records and using the Rainflow Counting method. Experimental measurements identified an almost 50% decrease in the residual life of one of the girders. It was caused by non-compliance with the technological procedures for the regular replacement of the rails, where the rail was welded to the top flange on one of the girders. Considering realistic operating conditions, predicting the effect of welded rail on fatigue damage accumulation, performed by other than experimental procedures, is almost impossible for such complex structures. This paper not only documents the importance of experimental measurements but also highlights the significance of selecting measurement locations with consideration of the current technical state of the structure.
Precoating Effects in Fine Steelmaking Dust Filtration
Authors: Andrade Bárbara K. S. A. , Sartim Rafael , Aguiar Mônica L.
Published: October 2022

Particle emissions into the atmosphere can cause extensive damage to the environment and human health. To improve the efficiency in the collection of submicronic particles, new filtration media appeared on the market due to new textile technologies and equipment, such as filter media developed with polytetrafluoroethylene (PTFE) membranes; however, these are more expensive. A filter coating technique called precoating is a more economical alternative that could enhance filtration efficiency. This paper aimed to evaluate the operational parameters of precoating for micro- and nanoparticle filtration and compare the results with those obtained from the PTFE membrane. For this purpose, filtration cycles were performed, using precoated polyester with hydrated lime and dolomitic limestone, polyester with a PTFE membrane, and steel industry ultrafine dust. The results showed that the precoated polyester had a longer cycle duration and lower pressure drop than the polyester with a PTFE membrane. Therefore, precoating was shown to be a great alternative to be used in bag filters in steel mills because it presented high collection efficiency for submicronic particles, in addition to increasing the bag lifespan with less energy expenditure.
A Multilevel Fuzzy Transform Method for High Resolution Image Compression
Authors: Di Martino Ferdinando , Sessa Salvatore
Published: October 2022

The Multilevel Fuzzy Transform technique (MF-tr) is a hierarchical image compression method based on Fuzzy Transform, which is successfully used to compress images and manage the information loss of the reconstructed image. Unlike other lossy image compression methods, it ensures that the quality of the reconstructed image is not lower than a prefixed threshold. However, this method is not suitable for compressing massive images due to the high processing times and memory usage. In this paper, we propose a variation of MF-tr for the compression of massive images. The image is divided into tiles, each of which is individually compressed using MF-tr; thereafter, the image is reconstructed by merging the decompressed tiles. Comparative tests performed on remote sensing images show that the proposed method provides better performance than MF-tr in terms of compression rate and CPU time. Moreover, comparison tests show that our method reconstructs the image with CPU times that are at least two times less than those obtained using the MF-tr algorithm.
Statistical Analysis of Alpha Power Exponential Parameters Using Progressive First-Failure Censoring with Applications
Authors: Nassar Mazen , Alotaibi Refah , Elshahhat Ahmed
Published: October 2022

This paper is an endeavor to investigate some estimation problems of the unknown parameters and some reliability measures of the alpha power exponential distribution in the presence of progressive first-failure censored data. In this regard, the classical and Bayesian approaches are considered to acquire the point and interval estimates of the different quantities. The maximum likelihood approach is proposed to obtain the estimates of the unknown parameters, reliability, and hazard rate functions. The approximate confidence intervals are also considered. The Bayes estimates are obtained by considering both symmetric and asymmetric loss functions. The Bayes estimates and the associated highest posterior density credible intervals are given by applying the Monte Carlo Markov Chain technique. Due to the complexity of the given estimators which cannot be compared theoretically, a simulation study is implemented to compare the performance of the different procedures. In addition, diverse optimality criteria are employed to pick the best progressive censoring plans. Two engineering applications are considered to illustrate the applicability of the offered estimators. The numerical outcomes showed that the Bayes estimates based on symmetric or asymmetric loss functions perform better than other estimates in terms of minimum root mean square errors and interval lengths.
Pan-Tissue and -Cancer Analysis of ROR1 and ROR2 Transcript Variants Identify Novel Functional Significance for an Alternative Splice Variant of ROR1
Authors: John Miya , Ford Caroline E.
Published: October 2022

ROR1/2 are putative druggable targets increasing in significance in translational oncology. Expression of ROR1/2 mRNA and transcript variants has not been systematically examined thus far. ROR1/2 transcript variant sequences, signal peptides for cell surface localisation, and mRNA and transcript variant expression were examined in 34 transcriptomic datasets including 33 cancer types and 54 non-diseased human tissues. ROR1/2 have four and eight transcript variants, respectively. ROR1/2 mRNA and transcript variant expression was detected in various non-diseased tissues. Our analysis identifies predominant expression of ROR1 transcript variant ENST00000545203, which lacks a signal peptide for cell surface localisation, rather than the predicted principal variant ENST00000371079. ENST00000375708 is the predominantly expressed transcript variant of ROR2. ROR1/2 expression in healthy human tissues should be carefully considered for safety assessment of targeted therapy. Studies exploring the function and significance of the predominantly expressed ROR1 transcript variant ENST00000545203 are warranted.
High Pretreatment Serum PD-L1 Levels Are Associated with Muscle Invasion and Shorter Survival in Upper Tract Urothelial Carcinoma
Authors: Széles Ádám , Kovács Petra Terézia , Csizmarik Anita , Váradi Melinda , Riesz Péter , Fazekas Tamás , Váncsa Szilárd , Hegyi Péter , Oláh Csilla , Tschirdewahn Stephan , Darr Christopher , Krafft Ulrich , Grünwald Viktor , Hadaschik Boris , Horváth Orsolya , Nyirády Péter , Szarvas Tibor
Published: October 2022

Programmed death ligand-1 (PD-L1) is an immune checkpoint molecule and a widely used therapeutic target in urothelial cancer. Its circulating, soluble levels (sPD-L1) were recently suggested to be associated with the presence and prognosis of various malignancies but have not yet been investigated in upper tract urothelial carcinoma (UTUC). In this study, we assessed sPD-L1 levels in 97 prospectively collected serum samples from 61 UTUC patients who underwent radical nephroureterectomy (RNU), chemotherapy (CTX), or immune checkpoint inhibitor (ICI) therapy. In addition to pretreatment samples, postoperative and on-treatment sPD-L1 levels were determined in some patients by using ELISA. In the RNU group, elevated preoperative sPD-L1 was associated with a higher tumor grade (p = 0.019), stage (p < 0.001) and the presence of metastasis (p = 0.002). High sPD-L1 levels were significantly associated with worse survival in both the RNU and CTX cohorts. sPD-L1 levels were significantly elevated in postoperative samples (p = 0.011), while they remained unchanged during CTX. Interestingly, ICI treatment caused a strong, 25-fold increase in sPD-L1 (p < 0.001). Our results suggest that elevated preoperative sPD-L1 level is a predictor of higher pathological tumor stage and worse survival in UTUC, which therefore may help to optimize therapeutic decision-making. The observed characteristic sPD-L1 flare during immune checkpoint inhibitor therapy may have clinical significance.
Visual-Based Spatial Coordinate Dominates Probabilistic Multisensory Inference in Macaque MST-d Disparity Encoding
Authors: Zhang Jiawei , Huang Mingyi , Gu Yong , Chen Aihua , Yu Yuguo
Published: October 2022

Numerous studies have demonstrated that animal brains accurately infer whether multisensory stimuli are from a common source or separate sources. Previous work proposed that the multisensory neurons in the dorsal medial superior temporal area (MST-d) serve as integration or separation encoders determined by the tuning–response ratio. However, it remains unclear whether MST-d neurons mainly take a sense input as a spatial coordinate reference for carrying out multisensory integration or separation. Our experimental analysis shows that the preferred tuning response to visual input is generally larger than vestibular according to the Macaque MST-d neuronal recordings. This may be crucial to serving as the base of coordinate reference when the subject perceives moving direction information from two senses. By constructing a flexible Monte-Carlo probabilistic sampling (fMCS) model, we validate this hypothesis that the visual and vestibular cues are more likely to be integrated into a visual-based coordinate rather than vestibular. Furthermore, the property of the tuning gradient also affects decision-making regarding whether the cues should be integrated or not. To a dominant modality, an effective decision is produced by a steep response-tuning gradient of the corresponding neurons, while to a subordinate modality a steep tuning gradient produces a rigid decision with a significant bias to either integration or separation. This work proposes that the tuning response amplitude and tuning gradient jointly modulate which modality serves as the base coordinate for the reference frame and the direction change with which modality is decoded effectively.
Oligometastatic Breast Cancer Patients Treated with High-Dose Chemotherapy and Targeted Radiation: Long-Term Follow-Up of a Phase II Trial
Authors: Ladbury Colton , Hao Claire , Ruel Christopher , Liu Jason , Glaser Scott , Amini Arya , Wong Jeffrey , Paz Isaac , Leong Lucille , Morgan Robert , Margolin Kim , Shibata Stephen , Frankel Paul , Somlo George , Dandapani Savita
Published: October 2022

Background: Patients with oligometastatic breast cancer (oMBC) may benefit from aggressive local therapy. We sought to assess the effects of consolidative radiation therapy (RT) on outcomes in oMBC patients treated on a prospective phase II trial of high-dose chemotherapy (HDCT). Methods: Between 2005 and 2009, 12 patients with oMBC (≤3 metastatic sites) cancer were treated on protocol. Patients were to receive tandem HDCT supported by hematopoietic cell rescue (HCR). All radiographically identifiable oligometastatic sites received targeted radiation. Results: HDCT was initiated at a median of 6.7 (3.5–12.7) months after diagnosis of oMBC. Hormone receptors (HR) were positive in 91.6% of patients, and HER2 was overexpressed in 25% of patients. Median radiation dose (EQD2) was 41.2 (37.9–48.7) Gy. Median follow-up was 13.1 (6.8–15.1) years for living patients. Ten-year PFS and OS were 33% (95%CI, 10–59%) and 55% (95%CI, 22–79%), respectively. Durable local control of treated lesions was 87.5%. At the last follow up, two patients remained progression free and two more were without evidence of disease following additional salvage treatment. Conclusions: Although modern systemic therapies have obviated the use of HDC, aggressive local therapy warrants further evaluation and fractionated radiotherapy is a viable alternative if SBRT is not available.
Prognostic Impact of MAFLD Following Surgical Resection of Hepatitis B Virus-Related Hepatocellular Carcinoma: A Nationwide Cohort Study
Authors: Yun Byungyoon , Ahn Sang Hoon , Oh Juyeon , Yoon Jin-Ha , Kim Beom Kyung
Published: October 2022

The association between the metabolic effects of hepatic steatosis as a part of postoperative outcomes of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) has rarely been studied. This study aimed to assess the relationship between metabolic dysfunction-associated fatty liver disease (MAFLD) and patients’ prognoses following curative resection of HBV-related HCC. Patients who underwent surgical resection for HBV-related HCC between 2009 and 2015 were recruited. The study endpoints were postoperative hepatocellular carcinoma (HCC) recurrence and all-cause mortality. Adjusted hazard ratios (aHRs) for the outcomes were estimated using multivariate Cox regression models. The mean age of the 2032 enrolled patients was 55.0 years, and 77.9% were men. During follow-up (median 5.3 years), HCC recurrence and all-cause mortality occurred in 954 (47.0%) and 422 (20.8%) patients, respectively. HCC recurrence and all-cause mortality were significantly associated with MAFLD, with aHRs of 1.22 (p = 0.003) and 1.44 (p < 0.001), respectively. Propensity score matching and inverse probability treatment weighting analyses confirmed similar results (p < 0.05). MAFLD was associated with significantly poor prognoses in terms of HCC recurrence and all-cause mortality following surgical resection of HBV-related HCC. Further studies are needed to develop an effective preventive strategy through the management of metabolic health.
Treatment of Refractory Metastatic Renal Cell Carcinoma
Authors: Vento Joseph A. , Rini Brian I.
Published: October 2022

First-line treatment for metastatic renal cell carcinoma (mRCC) rapidly shifted in recent years with the advent of combination therapies, including immune checkpoint inhibitor (ICI) doublets and combinations of an ICI with a vascular endothelial growth factor receptor (VEGFR) targeted tyrosine kinase inhibitor (TKI). Despite improvements in overall survival and many durable responses, there exists a significant number of patients who fail to respond to these agents, and many patients eventually progress. Given the rapid changes in the front-line setting, it is essential to understand treatment options in refractory mRCC. Here, we review the evidence behind current options for later-line therapies, often involving additional VEGFR-TKIs alone or in combination with mammalian target of rapamycin (mTOR) targeted agents, as well as situations where consideration of immunotherapy rechallenge may be appropriate. Additionally, we describe ongoing clinical trials examining concurrent ICI and TKI in the refractory setting, as well as those studying novel agents, such as targeted drug–antibody conjugates and hypoxia inducible factor 2α (HIF-2α) inhibitors. Finally, we review considerations for non-clear cell histologies in the refractory setting and mechanisms of resistance in mRCC.