bluuebunny/crossref_metadata_2025 huggingface dataset:
3.2 Nucleofection of iPSCs v1
Authors: J. Sauter Evelyn , K. Kutsche Lisa , D. Klapper Simon , Busskamp Volker
Published: September 2021

This is part 3.2 of the "Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders" collection of protocols. Collection Abstract: Patient-derived or genomically modified human induced pluripotent stem cells (iPSCs) offer the opportunity to study neurodevelopmental and neurodegenerative disorders. Overexpression of certain neurogenic transcription factors (TFs) in iPSCs can induce efficient differentiation into homogeneous populations of the disease-relevant neuronal cell types. Here we provide protocols for genomic manipulations of iPSCs by CRISPR/Cas9. We also introduce two methods, based on lentiviral delivery and the piggyBac transposon system, to stably integrate neurogenic TFs into human iPSCs. Furthermore, we describe the TF-mediated neuronal differentiation and maturation in combination with astrocyte cocultures.
3.4 Genome Editing with CRISPR/Cas9 v1
Authors: J. Sauter Evelyn , K. Kutsche Lisa , D. Klapper Simon , Busskamp Volker
Published: September 2021

This is part 3.4 of the "Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders" collection of protocols. Collection Abstract: Patient-derived or genomically modified human induced pluripotent stem cell s (iPSCs) offer the opportunity to study neurodevelopmental and neurodegenerative disorders. Overexpression of certain neurogenic transcription factors (TFs) in iPSCs can induce efficient differentiation into homogeneous populations of the disease-relevant neuronal cell types. Here we provide protocols for genomic manipulations of iPSCs by CRISPR/Cas9. We also introduce two methods, based on lentiviral delivery and the piggyBac transposon system, to stably integrate neurogenic TFs into human iPSCs. Furthermore, we describe the TF-mediated neuronal differentiation and maturation in combination with astrocyte cocultures.
Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer v1
Authors: B. Robers Matthew , D. Vasta James , R. Corona Cesear , Friedman Ohana Rachel , Hurst Robin , A. Jhala Manisha , M. Comess Kenneth , V. Wood Keith
Published: September 2021

Intracellular target affinity and residence time are fundamental aspects of pharmacological mechanism (Lu and Tonge, Curr Opin Chem Biol 14:467–474, 2010). Although various robust biochemical approaches exist to measure these binding characteristics, analysis of compound binding with isolated targets may not accurately reflect engagement in the milieu of living cells. To realize the influence of cellular context, methods are needed that are capable of quantifying affinity and residence time in the presence of the intracellular factors that may impact target engagement. Bioluminescence resonance energy transfer (BRET) offers a solution for intracellular target engagement when quantitative metrics or kinetic analyses are required.
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle v1
Authors: S. Hüttner Sören , E. Ahrens Hellen , Schmidt Manuel , Henze Henriette , Juliane Jung Marie , C. Schüler Svenja , von Maltzahn Julia
Published: September 2021

The isolation and culture of single floating myofibers with their adjacent muscle stem cells allow the analysis and comparison of muscle stem cells from aged and young mice. This method has the advantage that muscle stem cells are cultured on the myofiber, thereby culturing them in conditions as close to their endogenous niche as possible. Here we describe the isolation, culture, transfection with siRNA, and subsequent immunostaining for muscle stem cells on their adjacent myofibers from aged and young mice.
Evolution of Viral Genomes: Interplay Between Selection, Recombination, and Other Forces v1
Authors: J. Spielman Stephanie , Weaver Steven , D. Shank Stephen , Rife Magalis Brittany , Li Michael , L. Kosakovsky Pond Sergei
Published: September 2021

Natural selection is a fundamental force shaping organismal evolution, as it both maintains function and enables adaptation and innovation. Viruses, with their typically short and largely coding genomes, experience strong and diverse selective forces, sometimes acting on timescales that can be directly measured. These selection pressures emerge from an antagonistic interplay between rapidly changing fitness requirements (immune and antiviral responses from hosts, transmission between hosts, or colonization of new host species) and functional imperatives (the ability to infect hosts or host cells and replicate within hosts). Indeed, computational methods to quantify these evolutionary forces using molecular sequence data were initially, dating back to the 1980s, applied to the study of viral pathogens. This preference largely emerged because the strong selective forces are easiest to detect in viruses, and, of course, viruses have clear biomedical relevance. Recent commoditization of affordable high-throughput sequencing has made it possible to generate truly massive genomic data sets, on which powerful and accurate methods can yield a very detailed depiction of when, where, and (sometimes) how viral pathogens respond to various selective forces. Here, we present recent statistical developments and state-of-the-art methods to identify and characterize these selection pressures from protein-coding sequence alignments and phylogenies. Methods described here can reveal critical information about various evolutionary regimes, including whole-gene selection, lineage-specific selection, and site-specific selection acting upon viral genomes, while accounting for confounding biological processes, such as recombination and variation in mutation rates.
NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7660 -- Express Protocol without PCR Bead Cleanup and Varskip Primers v1
Authors: Gautreau Isabel
Published: September 2021

This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions and the NEBNext VarSkip Short SARS-CoV-2 Primer Mix 1 and 2 released in Sep 2021. This protocol does not include a cleanup and normalization step for each sample after cDNA synthesis. Performing the cleanup and normalization step creates library pools where the reads for each library are more evenly distributed. Skipping these steps reduces hands on time, but may require a longer sequencing run to obtain sufficient coverage for each sample. To obtain more even sample to sample coverage, we recommend normalizing the RNA samples prior to starting the protocol. To obtain instructions for using NEBNext VarSkip Short SARS-CoV-2 Primer Mix and the NEBNext® ARTIC SARS-CoV-2 Companion Kit STANDARD workflow (with cleanup after PCR) please contact NEB using info@neb.com. For other NEBNext® ARTIC SARS-CoV-2 protocols, please see the NEBNext ARTIC Protocols Collection.
MicroCT protocols for scanning and 3D analysis of adult Hexaplex trunculus v1
Authors: Chatzinikolaou Eva , Keklikoglou Kleoniki
Published: September 2021

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning and 3D analysis for the marine gastropod species Hexaplex trunculus.
Making electro-competent cells v1
Authors: not provided Ashwinuday
Published: September 2021

This protocol can be used for obtaining of electro-competent bacterial cells.
Interrogation in Nigerian Police-suspect Discourse
Authors: Olanrewaju Farinde, Raifu , Ademola Oyedokun-Alli, Wasiu , Obinna Iroegbu
Published: September 2021

Police-suspect interrogation is a peculiar discourse genre where there is interplay of power and dominance. This study examines the process by which linguistic coercion by the police is being actualized. The data for the study were interrogations between the police and some suspects that were selected within Ondo and Ekiti State Police Commands. The towns were purposively chosen based on their geographical spread across the Ondo Area Command as well as their population and commercial advantages. The data gathered were transcribed and analysed using Gibbons’ Forensic Questioning model. Since police-suspect interaction is based on questions and answers, questions in police-suspect interaction were classified according to the degree of power embedded in them. The result indicated that assault, abduction, affray and robbery were the common themes in Police-Suspect Discourse. The findings of the study reveal that in Police-Suspect Discourse there are such questions as declarative, choice, restricted and non-restricted WH-questions, special formulas and projected questions. The study further reveals that the questioning strategies of the police afford them the opportunity of controlling the interrogation.  The more frequently the police use questions, the more they are coercive.
The Semantic Structure of Color Terms in Arabic: A Cognitive Approach
Authors: Al-Tamimi Jinan
Published: September 2021

The acquisition of the ability of perceiving and naming colors through language is an important topic in which languages vary and differ. The construction of color concepts and naming them are directly influenced by the culture and environment of each society. This can be noted by observing two aspects: Cognitive Semantics and its effect on the collective mind. This study focuses on the cognitive foundations of color terms in Arabic, and the semantic relation between the color concepts and terms in selected examples from both old and new usage of these color terms in Arabic. The study aims to cover the most dominant semantic components for color terms in the Arabic language, using the cognitive linguistic approach and the descriptive analytics method to determine the structure of cognitive perception of color terms in a language. Furthermore, the study stands on two pillars; the first reveals the way the conceptualization pattern of color terms occurs in Arab mindset displayed through selected examples of theoretical data on cognitive semantics, whereas the second addresses the semantic principle of color classification in Arabic. Finally, the conclusion, confirming the results about the notion that color naming in Arabic is based on the visual images associated with the colors in Arab environment, related to night and day. Hence, the color term becomes connected in the Arab mindset with the visual image, and under each color are colors similar to it in hue.
Translation Alternatives of Indonesian Public Signs
Authors: Susini Made , Sujaya Nyoman , Ana I Wayan
Published: September 2021

Public signs written in two languages are found everywhere in public places in Bali. Appropriate translations of the public signs could help foreigners get information and they also could reflect good image of the places. To be able to create better image of the place, deep investigation on the translations of public signs is needed. This study examined the translations of public signs from Indonesian into English found in Bali. Deploying the concept of translation equivalence by House (2015) and translation strategies by Malone (1988), this study focused on target text alternatives when a certain strategy was applied in translating public signs from Indonesian into English. The data were collected in two regions in Bali, i.e. Denpasar city and Badung regency. This research revealed that the Indonesian public signs were translated by applying strategies of substitution, amplification, reduction, condensation, and re-ordering. The strategies resulted in some translation alternatives. In some cases, the alternatives induced by the translation strategies applied involved the changes of pragmatic and textual meanings. However, they are semantically equivalent.
ICC Nurturing Strategies in the Context of Sino-foreign Institutes
Authors: Zhu Xiaomei , Feng Anwei
Published: September 2021

This paper discusses intercultural communicative competence (ICC) education in the context of Sino-Foreign Institutes (SFIs). Through an overview of the development of internationalization of higher education in China, the paper puts forward four strategies that are widely adopted to facilitate the development of students ICC. The four strategies are provision of ICC specific courses, integration of ICC in subject courses, integration of ICC with foreign language education, and intercultural activities and projects. Towards the end, the paper argues that more empirical research is needed to evaluate the effects of the strategies on students’ ICC and challenges the SFIs face in the post-pandemic era.
Analysis of Speech Humor in Crosstalk From Principles of Cooperation and Relevance Theory
Authors: Li Xia
Published: September 2021

Cross talk is one of the unique performing arts in China, and most crosstalk gives the audience happy feelings through the delivery of humorous language. This paper interprets the generation of humor in crosstalk through the cooperation principles and the relevance theory, and the generation of the humorous results comes from the contrast between ostensive-inference model and the contrast between maximal relevance and optimal relevance.
A study to observe quantitative levels of C- Reactive Protein in Cerebrospinal fluid in patients with Meningitis as an add-on routine investigation- a case-control study
Authors:   M.D., Assistant Professor, Department of Medicine, Hind Institute of Medical Sciences, Safedabad, Barabanki, Uttar Pradesh, India, Kumar Manoj , Tiwari Chandrashekhar ,   Hind Institute of Medical Sciences, Prabhat Nandita ,   D.M., (Neurology), Assistant Professor, Department of Medicine, Hind Institute of Medical Sciences, Safedabad, Barabanki, Uttar Pradesh, India, Dhaon Pooja ,   M.D., Associate Professor, Department of Medicine, Hind Institute of Medical Sciences, Safedabad, Barabanki, Uttar Pradesh, India, Uraiya Dharmendra ,   M.D., Professor, Department of Medicine, Hind Institute of Medical Sciences, Safedabad, Barabanki, Uttar Pradesh, India
Published: September 2021

Introduction: C-reactive protein (CRP) is a member of the class of acute phase reactants as itslevel rises dramatically during inflammatory processes occurring in the body. Measuring and chartingCRP values can prove useful in determining the disease progress. Aim: To estimate the CRP level inCerebrospinal fluid (CSF) of patients with meningitis; and to evaluate whether CRP levels could beused to differentiate the various types of meningitis in adults. Materials and Methods: This studywas a case-control study. 80 enrolled patients were subjected to a protocol that included detailedclinical history including duration of illness, symptoms and signs, history or any treatment history.Written informed consent was taken from the patients/guardian. Results: Meningitis was morecommon in the 18-30 years age group. Mean values of CSF CRP were- viral meningitis (2.70 mg/L)and pyogenic meningitis (91.13 mg/L) and control group (1.54 mg/L). CSF CRP can be used as adiagnostic tool to differentiate between pyogenic and viral meningitis as it is significantly raised inpyogenic meningitis in comparison to viral meningitis (p-value <0.0001). Conclusion: CRP in CSF isa valuable, rapid, bedside diagnostic test for differentiating between pyogenic and viral meningitis;with reasonably good sensitivity, specificity and positive predictive value. The absence of CRP in CSFrather than its presence is more important for the diagnosis of viral meningitis.
FACTORS OF EFFICIENCY OF RESOURCE POTENTIAL MANAGEMENT HIGHER EDUCATIONAL ORGANIZATIONS
Authors:   Siberian Federal University, Baguzova Larisa
Published: September 2021

The article examines the factors that have a significant impact on the efficiency of resource potential management of higher education institutions. The positive impact of which ensures competitiveness and performance. The aim of the study is to analyze the study of the external and internal environment factors and the compilation of their classification. The multiplicity of studies and different approaches is of interest to this problem. For the study, such methods were used as analysis of the literature on the relevant topic, comparison, contrast. The results of the study will reduce the risks for identifying opportunities and reserves the growth of educational organizations. The scientific contribution of the author lies in the fact that the studied experience can be used and applied in the process of carrying out educational activities.
PRESENCE OF PUBLIC CATERING COMPANIES OF THE KRASNOYARSK REGION ON THE INTERNET IN 2021
Authors:   Siberian Federal University, Cacorin Aleksandr
Published: September 2021

The article examines the presence of public catering enterprises of the Krasnoyarsk Territory on the Internet in 2021. The research is based on the use of information data provided by the City Information System "2GIS" about the presence of companies, information about which is contained on the platform "2GIS". Among the data obtained, we selected those enterprises that provide catering services on the territory of the Krasnoyarsk Territory. The purpose of this work is to identify the share of public catering enterprises in the Krasnoyarsk Territory that have their own website or page on social networks to promote their services. In the course of the study, analytical methods of information processing were used. The number of surveyed enterprises (branches) is 2354. As a result of the study, it was found that the use of online tools in the promotion of services (sites, pages on social networks) allows companies to develop more efficiently than those organizations that are not present on the Internet. In the course of the study, the financial component of specific enterprises, the number of employees, the time of presence on the market, and marketing strategy were not assessed. The assessment was carried out solely according to the parameters of the presence / absence of a site and / or a page in social networks.
Automation of Control of Technological Process of Storage in a Granary with Horizontal Silos
Authors: Astapenko N. V. , Koshekov K. T.
Published: September 2021

Grain storage and processing is the most important component of the grain market infrastructure. The task of grain storage is to ensure complete safety of quantity and quality with minimal labor and money costs. In the laboratory of the M. Kozybayev North Kazakhstan University, a prototype of an innovative type of grain storage was developed using horizontal silos, designed to reduce the cost of construction and reconstruction of elevators while improving quality, increasing storage times and reducing current storage costs. The design feature makes it possible to automate most of the operations of the technological process of grain storage. This article proposes a method for the automated formation of control actions on grain storage equipment as a result of data analysis from temperature, humidity, carbon dioxide level sensors and 3D measurements of the grain surface. A project of an automated system for monitoring and controlling grain storage facilities with horizontal silos has been developed. A distinctive feature of the proposed automated system is the most complete set of conditions for the correct storage of grain in comparison with other control systems for the technological process of a granary. Automation of work is achieved through the developed method for generating control signals, as well as such computational algorithms as forecasting the completion time of loading based on the conditional filling rate of the grain storage section, forecasting the completion time of unloading based on the conditional grain release rate, calculating the grain volume in the section using 3D measurements. The logic of the functioning of the basic algorithms for an automated monitoring and control system for a granary with horizontal silos is based on the analysis of the parameters of temperature, humidity, carbon dioxide level and 3D measurements of the grain surface in the sections of the granary. In the presented project of an automated system, scientific knowledge and achievements in the field of grain storage and the theory of identification measurements were taken into account, which makes it possible to use the described algorithms in information systems for monitoring grain storage facilities of other types.
Etalon for Nanometrology Produced by Anisotropic Etching
Authors:   A. M. Prokhorov General Physics Institute, Russian academy of Science, Moscow, 119991, Russian Federation, Larionov Yu.V.
Published: September 2021

Possibilities and results of using relief structures produced by anisotropic etching as etalons for linear measurements in nanorange are discussed. Two types of the structures with different profiles and with two approaches to estimation of influence of its sophistication degree on measurement results are considered. Analyze of methods and means of measurements of its geometric parameters and comparison of uncertainness of measurement results are conducted. The important condition for diminishing the measurements uncertainness is taking into account irregularities of a sample surface. These irregularities were evaluated by TEM and CD-AFM. Results of harnessing these types of structures are different also. The bureau International des Poids et Mesures recommended the structure with rectangular profile and its attestation procedure as an example for all national committees on linewidth metrology in nanorange. The structure with trapezoid profile that induced hopes for decision of basic tasks of nanometrology in past is occurred to be poorly in demand even on national scale.
Modification of the Adsorption Properties of Nanomaterials Based on Zinc Oxide and Tin Dioxide after Additional Activation in Argon Plasma
Authors:   Penza State University, Penza, 440026, Russian Federation, Meshcheriakov A.V. , Sigaev A.P. ,   Penza State University, Penza, 440026, Russian Federation, Averin I.A. ,   Penza State University, Penza, 440026, Russian Federation, Karmanov A.A. ,   Penza State University, Penza, 440026, Russian Federation, Pronin I.A. ,   Penza State University, Penza, 440026, Russian Federation, Yakushova N.D. ,   Penza State University, Penza, 440026, Russian Federation
Published: September 2021

The modification of the adsorption properties of nanomaterials based on ZnO and SnO2, synthesized by the sol-gel method, after additional activation of their surface by argon plasma has been studied. Correlation between the adsorption properties and photocatalytic activity has been established. The scanning electron microscopy has confirmed the degradation of structure of nanomaterials with high-power plasma source and/or prolonged activation, and as a consequence, reduction of their photocatalytic properties
Let’s Get Loud: Intersectionally Studying the Super Bowl’s Halftime Show
Authors: Van Bauwel Sofie , Krijnen Tonny
Published: September 2021

The study of popular culture has always been closely related to the study of class, gender, race, and sexuality. An increasing number of authors have called for an intersectional approach. However, the contradictory, fluid meanings articulated in popular culture render such an approach difficult, and many ignore the call for intersectional analysis. We will not. We will try to engage with an intersectional analysis of popular culture, using Shakira and Jennifer Lopez’s performance at the 2020 Super Bowl Halftime Show as a case to study the intersections of identity markers. We aim to bridge the different meanings attributed to their performance and to understand them as different elements in the intersectional configuration. A discourse analysis of the performance, and of reviews thereof, was performed to unravel five elements highlighted in the discourse: the quality of the show, Shakira and Lopez’s empowered performances, the incorporation of Latinidad elements, the performers’ sexiness, and perceived political messages. Our aim to understand how the contradictory discourses about these elements arose urges the reader to use listening to grapple with the complexity of intersectional analysis. Truly listening includes putting effort into opening up academic cultures, finding other voices. It is important to recognize global gender inequity, but we need to start investing far more to understand the politics of media representations as a transnational affair that causes multiple conceptions of gender (and other related) concepts to clash, mesh, and integrate.
Coping strategies associated to widespread fear and post-traumatic stress criteria in a Mexican sample during COVID-19 pandemic: A cross-sectional study
Authors: Chávez-Valdez Sarah Margarita , Domínguez Rodríguez Alejandro , Esparza-Del Villar Oscar Armando , Hernández Jiménez Ma. Jesús , Rosa-Gómez Anabel de la , Arenas-Landgrave Paulina , Martínez-Luna Sofía Cristina , Ramírez-Martínez Flor Rocío , Salinas-Saldívar Jasshel Teresa
Published: September 2021

Introduction. The COVID-19 pandemic has impacted the population generating widespread fear due to people’s perception that both their health and safety are at risk. Widespread fear, a synonym for social anxiety, is associated with affective intrusive cognitions. Likewise, it is related to symptoms of post-traumatic stress, physiological intrusive and eluding coping responses, and could be associated with long-term vulnerability to develop PTSD. Objective. To assess the association between coping strategies, widespread fear to COVID-19, and symptoms of post-traumatic stress disorder in Mexican population exposed to the SARS-CoV2 pandemic. Method. A cross sectional, empirical, descriptive study through survey research was performed; 370 participants were sampled by nationality quotas, and they completed digital self-informed instruments on an on-line psychosocial intervention. Sample size was estimated using a power analysis. Results. Findings showed reliable indicators between physiological coping and PTSD symptoms. There was a 42% variance related to PTSD delayed intrusive cognitive responses, a 40% variance was related to PTSD negative alterations in cognitions and mood, a 31% variance was explained by hyperarousal responses, and a 29% of the variance represented PTSD avoidance symptoms. Widespread fear of COVID-19 was explained by affective behavioral coping strategies linked also to PTSD symptoms. Discussion and conclusion. Research found consistency between findings and theories on emotion-centered strategies, which in turn were are related to affective coping around widespread fear to COVID-19 and avoidance intrusive symptoms, physiological coping with avoidance, considering future community post-traumatic stress traits and anxiety disorders in Mexican communities exposed to the pandemic, linking strategies with community trauma.
Positive reappraisal as a stress coping strategy during the COVID-19 pandemic
Authors: Garza Varela Juan Pablo , Cruz-de la Cruz Carlos de la , Leija Guerrero Jessica Giselle , Sánchez Rodríguez Karen Estefanía , Kawas Valle Omar
Published: September 2021

Introduction. The stress coping strategies people rely on impact their psychological well-being and may be related to the risk of developing psychopathology in the short- and long-term after an unfortunate event. Objective. To define the main stress coping strategies in our region as a first step in assessing the risk of developing psychopathology derived from the SARS-CoV-2 pandemic. Method. This is an observational, cross-sectional, analytical study that includes 396 participants over 18 years of age residents of the state of Nuevo Leon. Using an online form, the participants were asked to complete the Coping Strategies Questionnaire (CAE). Results. In general, after two months of mitigation measures in Mexico, positive reappraisal was the stress coping strategy with the highest score in the population studied. We found higher scores on negative auto-focused and overt emotional expression on the comparation between sociodemographic subgroups. Discussion and conclusion. The present study shows that, in our community, positive reappraisal was the stress coping strategy with the highest score. Positive reappraisal is an active form of coping associated with greater psychological well-being and fewer risk of developing psychopathology in the short and long term. Based on this, our hypothesis is that the predominant use of this strategy could translate into a higher psychological well-being during and after the SARS-CoV-2 pandemic in the studied sample.
Impact of the use of sexual material and online sexual activity during preventive social isolation due to COVID-19
Authors: Hernández-Torres José Luis , Rodríguez-Vázquez Nancy , Martínez-Cervantes Roxana , Mayela Cárdenas-Cortés Adriana , Gómez-Melasio Dafne Astrid , Trujillo-Hernández Pedro Enrique
Published: September 2021

Introduction. Preventive social isolation due to coronavirus disease (COVID-19) has represented one of the greatest health challenges of the last decades worldwide. As a result of social isolation, the consumption of information in digital media, such as the use of online sexual material, has increased, leading to risky sexual behavior in young people. Objective. To quantify the impact on the use and type of online sexual material and to determine the predictors of online sexual activity in people in preventive social isolation due to COVID-19. Method. Multivariate cross-sectional study; 385 participants were studied and contacted through an online survey. Results. Internet pages and social networks are the main platforms for the use of online sexual material, and its consumption was more frequent in those who had more days of preventive social isolation. Predictors of sexual activity were cybersex (β = .38), excitation (β = .36), masturbation (β = .34), and adventure (β = .33), which were found to be statistically significant (p ˂ .001). Discussion and conclusion. Privacy plays an important role in the use of online sexual material and activities, and greater consumption can be found in intimacy. It is important to be alert to the effects of the pandemic on sexual risk behavior and further research is needed.
Sürekli Mıknatıslı Senkron Motorun Alan Yönlendirmeli Kontrolü ve Kontrol İçin Geliştirilen Özel Amaçlı Motor Sürücüsünün Tasarımı ve Prototip Üretimi
Authors: YILMAZ Hüseyin Cemre , KANDEMİR BEŞER Esra
Published: September 2021

Son dönemde elektrik makinaları alanında yapılan çalışmalar neticesinde popülerlik kazanan sürekli mıknatıslı senkron motorlar (SMSM), günümüzde endüstriyel uygulamalardan son kullanıcı ürünlerine kadar geniş bir kullanım alanına sahiptir. Sürekli mıknatıslı senkron motorların uygun kontrolü ile endüstride sıklıkla karşılaşılan birçok uygulama, robotik sistemler, talaşlı imalatın temel yapı taşı haline gelmiş metal işleme tezgâhları vb. uygulamalarda, düşük hacimde yüksek performans elde etmek mümkün hale gelmiştir. Bunun yanı sıra yine endüstride karşılaşılan yüksek dinamizm ve/veya hassas pozisyon kontrolü gerektiren uygulamalarda da sıklıkla sürekli mıknatıslı senkron motorlar tercih edilmektedir. Sürekli mıknatıslı senkron motorların yüksek performanslı kontrolünü sağlamak amacıyla geliştirilen birçok yöntem bulunmakla birlikte, bunlardan en çok öne çıkan iki tanesi Alan Yönlendirmeli Kontrol ve Doğrudan Moment Kontrolü teknikleridir. Alan yönlendirmeli kontrol tekniğinin pozisyon geri besleme elemanı ile birlikte uygulanması durumunda çoğu uygulamada ihtiyaç duyulan yüksek kalkış momenti, düşük devirlerde yüksek hız kararlılığı ve farklı hız referans değerlerine karşı dinamik tepki ihtiyaçlarını karşılayabilmesi bu tekniğin birçok endüstriyel hız kontrol cihazında da tercih edilmesine sebep olmuştur. Bu çalışmada, alan kontrolü yapmak üzere geliştirilen mikrodenetleyici tabanlı motor sürücüsü üzerinde alan yönlendirmeli kontrol tekniği uygulanarak, miline artımsal enkoder bağlı bir sürekli mıknatıslı senkron motorun hız kontrolü ve ayrıca moment kontrolü sağlanmıştır. Hız kontrolü ve moment kontrolü yapılırken sistemin verilen referans değerlere karşı tepkisi ölçülmüştür. Yapılan ölçümler farklı kontrol parametreleri ile tekrarlanarak karşılaştırmalar yapılmıştır.
To the Berliner. On the question of the examination of paranoid mental illnesses after accidents. Zur Begutachtung paranoischer Geistesstörungen nach Unfällen, Klinik f. psych. u. nerv. Kranch. v. R. Sommer. B. V. H. 3. S. 224
Authors: Averbuch R.
Published: September 2021

Considering the issue of the ethological connection between the trauma and the mental illness that followed it, the author informs the story of the illness of patient G. , characterized by vague delusional ideas of a hypochondriacal nature and ideas of persecution, which seem to be in connection with deceptions of the senses. Along with a deep weakness of will and lack of initiative, the disease gave a picture of dementiae paranoides.
On the question of ethiology and pathogenesis of psychosthenia
Authors: Kipshidze C.
Published: September 2021

With the revival of psychotherapy, interest in neuroses is increasing more and more. New theories are being created, the old are hesitating, yielding to a new trend. Each, more or less pronounced case of neurosis is examined from a new angle of vision, confirming or refuting the still incomplete established data.
Surgical intervention for seizures of partial epilepsy caused by a brain tumor
Authors: Lapukhin V. D.
Published: September 2021

Patient S., whom I have the honor to present to the Society, was operated on in the nervous clinic of Kazan University for seizures of partial epilepsy, caused by neoplasms in the motor region of the right cerebral cortex. And since such cases are not frequent in the foreign medical press, and in Russia in particular, I use this case as a demonstration.
K. Yatsuta. Tripod for installation of skulls during measurements. Medical Newspaper No. 37, 1912
Authors: Averbuch R.
Published: September 2021

The author describes a crane invented by him, which allows prolonging all measurements of the skull with a constant, definite setting. This is achieved by the fact that the horizontal frame of the crane is easily removed, and the skull remains open and in the same position.
Mach Löwy. K casuisticѣ side effects when using medicinal mineral waters. Zur Kasúistik der Brunnenkrise. (des Brúnnendusesl des Brunnen und Baderansches) Zeitschrift für Balneologie, Klimatologie und Kurort — Hygiene. IV Iahrgang Nr. 12
Authors: Averbuch R.
Published: September 2021

The phenomena described by the author are in the nature of mental, nervous and circulatory disorders.
Modern drainage interventions in the surgical treatment pancreatic cyst
Authors: Lazutkin Maksim V. , Ivanusa Sergey Y. , Shershen Dmitriy P. , Popov Alexandr A.
Published: September 2021

Purpose. To evaluate the capabilities of modern minimally invasive interventions under the beam guidance in the surgical treatment of pancreatic cysts.

Materials and Methods. The results of the examination and treatment of 88 patients with pancreatic cysts. Percutaneous puncture drainage under ultrasonographic guidance holds 56 patients, endoscopic transmural drainage of cysts by ultrasonography- in 32.

Results. After performing percutaneous drainage of cysts positive results were observed in 42 (75,0%) patients. Relapses cystic formations were observed in 14 (25.0%) patients. Endoscopic transmural drainage under ultrasonography noted one intraoperative complication perforation of the stomach wall. 6 patients had a good result. Term follow up of patients ranged from 10 months to 3 years.

During endoscopic transmural drainage stent dislocation and relapse of cysts were not detected in all operated patients after 6 months, Long-term results were observed only in 11 patients.

Conclusions. Percutaneous external drainage of pancreatic cysts is an effective intervention in 75% of patients. A fairly high percentage (25%) of relapses is due to the fact that long-term external drainage do not allow to achieve complete obliteration of the cyst cavity in its connection with the main pancreatic duct and preservation of ductal hypertension of the pancreas. Endoscopic installation of a stent between the hand cavity and the lumen of the stomach allows you to create conditions for a constant outflow of cyst contents into the stomach and eliminate pancreatic hypertension. The effectiveness of internal drainage is determined by the duration of stent functioning, as well as the possibility of forming an internal cystogastric fistula (3 figs, bibliography: 8 refs).
The application of long frame draining of pancreatoejunoanastomosis in patient after gastropancreatoduodeonal resection
Authors: Ivanusa Sergey Y. , Shershen Dmitriy P. , Akiуev Rustam M. , Eliseev Alexander
Published: September 2021

Objective. To demonstrate the possibilities of early diagnosis of pancreatoejunonastomosis failure and options for surgical treatment of patients with pancreatic fistula after gastropancreatoduodenal resection using long-term frame drainage of pancreatojejunonastomosis.

Materials and methods. The results of combined treatment of a patient of the Department of General Surgery of S. M. Kirov Military Medical Academy with a intraductal papillary mucinous neoplasms. Pancreatoejunonastomosis failure was diagnosed based on the criteria of the International Research Group for the Study of Pancreatic Fistulas.

Results of the study. Patient B., 61 years old. In January 2019, computer tomography revealed a cystic cavity in the head of the pancreas. An controlled endoscopic ultrasound sonography biopsy was performed. Morphological examination verified the signs of intraductal papillary mucinous neoplasm. On March 11, 2019, the patient underwent gastropancreatoduodenal resection with decompression of pancreatoejunoanastomosis using long-term frame drainage of the main pancreatic duct. On the third day of the postoperative period, x-ray contrast fistulography was used to diagnose the failure of the pancreatoejunonastomosis with the formation of a B type fistula. On the 23d day after gastropancreatoduodenal resection, puncture drainage of the non-drained acute fluid collection was performed under ultrasonographic control. On the 36th day of the postoperative period the frame and puncture drains were removed.

Conclusion. The use of long-term frame drainage for decompression of the duct system with staged fistulography, early diagnosis of complications of gastropancreatoduodenal resection in the form of the formation of type B pancreatic fistula, provided the effectiveness of conservative and minimally invasive measures for the treatment of postoperative complications (8 figs, bibliography: 7 refs).
Blind multiple-input multiple-output system identification with applications in multiuser communications
Authors:   , Bradaric Ivan , Petropulu Athina P.
Published: September 2021

Multiple-Input Multiple-Output (MIMO) system models appear in many applications, such as telecommunications, earthquake engineering, speech processing and biomedical engineering. Therefore, MIMO system identification has attracted a lot of interest in recent years. The goal of blind identification of a (P x M) MIMO system is to identify an unknown system driven by P unobservable inputs, based on the M system outputs, and subsequently use the system estimate to recover the input signals (sources). In this thesis we propose a frequency domain approach for blind MIMO system identification, that uses second order spectra correlation of the system output. We first consider the case of stationary or cyclostationary, non-white inputs with known correlations. We propose a novel criterion that involves correlations of discrete Fourier transforms (DFT) of the system output, minimization of which yields the system impulse response within a scalar ambiguity. A strength of the proposed approach is insensitivity to channel length mismatch. The performance of the proposed method has been demonstrated via extensive simulations and also real measurements obtained using our wireless communications testbed. Next we consider the case where the input statistics are unknown, a problem of great interest in separation of competing speakers in speech processing applications. We show that under certain conditions, second order spectra correlations of the system output are sufficient for the unique identification of a P x P system. Although identifiability results for the same problem existed for the 2 x 2 case, this is the first result for the general P x P MIMO case. The case of white stationary inputs cannot be identified unless the higher-order statistics (HOS) are used. HOS have been criticized for high complexity and the need for long data in order to maintain small variance of the system estimates. We propose a low rank approach for reducing the variance of the HOS estimates for a fixed data length. We demonstrate the usefulness of the approach in Single-Input Single-Output (SISO) blind system identification.
The effects of plyometric training with additional weight applied to football athletes-students on some physical and physiological features and chronic muscle damage
Authors: Şirin Tayfun
Published: September 2021

The aim of this study is to investigate the effects of plyometric training with additional weight on physical performance and muscle damage findings in faculty of sports sciences athlete-students. Physical performance tests and blood samples of the athlete-students (male 23.41 ± 3.2 years old) were collected both from sample and the control groups at the first week of the study  and at the last training of the twelfth week. During the study, plyometric training programs were applied to the additional weight  group (AWG)  and no additional weight group (AG).  By the end of first and twelfth week, players’ BMI and body fat percentage tests were applied together with the physical tests of shuttle, yoyo, vertical jump, horizontal jump, agility and sprint performance tests. Blood samples were also taken to check muscle damage control markers (CK and LDH). Results: According to the results of tests performed at the end of first and twelfth weeks’ plyometric trainings on group with additional weight group (AWG), shuttle, yoyo and horizontal jumping were found to be significantly increased, while sprint performance, BMI and body fat performance tests were significantly decreased. The test results of the group without additional weight (AG) was; significant increase in shuttle, yoyo and vertical jump while significant decrease in sprint performance and fat %. By the end of twelve week plyometric trainings, no muscle damage has been observed in (AWG), CK pre-training (229,00 ± 155,25) and post training values (242,33 ± 193,67)  and LDH pre-training (169,75 ± 40,85) and post-training (174,33 ± 39,12) values.  As a result of the study, 12-week plyometric training with additional weight applied to football  athletes-students not only increased their physical performance, but also muscle damage (CK and LDH) was not observed.
Pensamiento Geométrico, Teoría de Van Hiele y Tecnologías Computacionales
Authors: Theran Eugenio
Published: September 2021

Introducción: En este artículo se muestran los resultados de la investigación “Estrategias didácticas para potenciar el pensamiento geométrico aplicando tecnologías computacionales y el modelo de Van Hiele”, que se desarrolló en una Institución Educativa del sector oficial del municipio de Corozal - Sucre, Colombia en el año 2016, fruto del trabajo de grado del autor en el marco de la Maestría en Educación del SUE Caribe, Sede Montería.

 

Objetivo: Con esta investigación se pretendió determinar la incidencia del empleo de estrategias didácticas en el desarrollo del pensamiento geométrico de los estudiantes, soportadas en Tecnologías Computacionales y el modelo de Van Hiele.

 

Método: Se utilizó un diseño cuasiexperimental, con métodos cuantitativos para establecer inferencias sobre el desempeño de los estudiantes al realizar las pruebas pretest y postest de validación de aprendizajes.

 

Resultados: Se puede señalar una mejora en los niveles de comprensión de la geometría en los estudiantes de grado sexto de una Institución Educativa del sector oficial del municipio de Corozal, Sucre, Colombia a través de la intervención con estrategias didácticas que emplearon Tecnologías Computacionales y el modelo de Van Hiele.

 

Conclusiones: El uso de la tecnología se convirtió en un elemento de valor relevante, los estudiantes mostraron mayor interés y evidenciaron una participación genuina y compromiso con su aprendizaje. El modelo de Van Hiele se validó como una herramienta para medir el avance que tuvieron los estudiantes en el aprendizaje de los cuadriláteros, transitando desde el nivel de visualización hasta el nivel de deducción informal.
Karyotypes and ZZ/ZW Sex Chromosome System of Endemic Squalius recurvirostris (Leuciscinae, Cyprinidae) in Turkey
Authors: DOORİ Ahmed Sadeq Jaber , ARSLAN Atilla
Published: September 2021

In this study, detailed cytogenetic properties of the endemic Akşehir chub, Squalius recurvirostris, distributed in the basin of the Ilgın (Çavuşçu) Lake basin was investigated by applying the standard Giemsa staining, C-banding and Ag-NOR technique. Diploid chromosome number is (2n) 50. A pair of heteromorphic chromosomes was detected in the karyotype in females. However, this difference was not observed in males. Therefore, it was determined that the heteromorphic chromosome pair in females were ZZ/ZW sex chromosomes. The Z chromosome in females is large acrocentric, and the W chromosome is medium-sized submeta/subtelocentric. The number of all chromosomal arms (NF) is 90, and the fundamental number of autosomal arms (NFa) is 88 in the karyotyped of the individuals studied. Some bi-armed and acrocentric chromosomes have centromeric C-bands in the chromosome set. The slightly centromeric or pericentromeric C-bands are observed in some chromosomes, while other chromosome pairs are C-negative. In the individuals studied two active NORs were detected. Both of these NORs were hemizygous and associated with a large C-heterochromatin region. One of the NORs was detected on the entire long arm of the submetacentric chromosome pair and the other on the entire short arm of another submetacentric chromosome pair.
Use of Olive-Mill Wastewater for the Containment of Phelipanche ramosa in Open Field of Processing Tomato Crop
Authors:   the Department of Agricultural Food and Environmental Science, University of Foggia, Italy, Lops Francesco , Carlucci Antonia , Frabboni Laura , Tarantino Annalisa , Disciglio Grazia
Published: September 2021

Phelipanche ramosa (L.) Pomel is a root holoparasitic weed that infested many crops, particularly the processing tomato. In semi-arid regions of the world, this problem is increasing, both in intensity and in acreages. In the last few years, researches for the control of this parasite has increased its effort to use suitable methods, as well as the utilization of natural compounds. In this regard, four experimental trials were carried out over three growing seasons (2014, 2016 and 2017), at Foggia (Apulia Region, Southern Italy), in the open field, on processing tomato (cv. “Dres”) crop, infested by P. ramosa, where the olive-mill wastewater (OMW) was incorporated into the soil at the dose of 80 m3 ha-1 40 days prior to tomato seedling transplant in comparison with the untreated control. The numbers of P. ramosa emerged shoots (branched plants) were significantly lower in the OMW treatment than in the untreated control. The tomato marketable yield showed a significantly higher value in the OMW treatment than the untreated control. No significant differences for the fruit qualitative characteristics were observed.