bluuebunny/crossref_metadata_2025 huggingface dataset:
Universal photonics tomography
Authors: Gaur Prabhav , Grieco Andrew , Alshamrani Naif , Almutairi Dhaifallah , Fainman Yeshaiahu
Published: April 2022

3D imaging is essential for the study and analysis of a wide variety of structures in numerous applications. Coherent photonic systems such as optical coherence tomography (OCT) and light detection and ranging (LiDAR) are state-of-the-art approaches, and their current implementation can operate in regimes that range from under a few millimeters to over more than a kilometer. We introduce a general method, which we call universal photonics tomography (UPT), for analyzing coherent tomography systems, in which conventional methods such as OCT and LiDAR may be viewed as special cases. We demonstrate a novel approach (to our knowledge) based on the use of phase modulation combined with multirate signal processing to collect positional information of objects beyond the Nyquist limits.
Optimising microring resonator based optical frequency comb distillation for optical communications systems
Authors: Prayoonyong Chawaphon , Corcoran Bill
Published: April 2022

Microring resonators (MRR) can be used as devices for filtering out broadband noise on optical frequency combs, in cases where significant amplification of a generated comb is required. While comb distillation has been demonstrated experimentally for optical communication systems, approaches to optimise device and sub-system parameters have not been explored. Here, we investigate how the performance of comb distillation through micro-ring filtering depends on device parameters. We also explore device parameter dependent performance when the comb and MRR are misaligned in line spacing. For the device platform we investigate, we find that the required optical signal-to-noise ratio (OSNR) of a comb line can be reduced by 16 dB, independent of modulation format, using a MRR with a resonance bandwidth of 100 MHz and coupling loss of 3 dB.
Simulation of Al0.85Ga0.15As0.56Sb0.44 avalanche photodiodes
Authors: Taylor-Mew J. D. , Petticrew J. D. , Tan C. H. , Ng J. S.
Published: April 2022

Al0.85Ga0.15As0.56Sb0.44 is a promising avalanche material for near infrared avalanche photodiodes (APDs) because they exhibit very low excess noise factors. However electric field dependence of ionization coefficients in this material have not been reported. We report a Simple Monte Carlo model for Al0.85Ga0.15As0.56Sb0.44, which was validated using reported experimental results of capacitance-voltage, avalanche multiplication and excess noise factors from five APDs. The model was used to produce effective ionization coefficients and threshold energies between 400–1200 kV.cm-1 at room temperature, which are suitable for use with less complex APD simulation models.
Integratable photodetectors based on photopolymerized conductive polymer via femtosecond laser direct writing
Authors: Liang Shuyu , Yang Ying , Lv Chao , Liu Yuefeng , Xia Hong
Published: April 2022

Conductive polymers have attracted a great deal of attention due to their remarkable electrical conductivity. However, the low solubility and inability to meet the limit for the flexible patterning fabrication ability of conductive polymers hinders their applications in miniaturized and integrated electronic devices. Here, femtosecond laser direct writing (FsLDW) is employed to achieve the in situ fabrication of polypyrrole (PPy) with flexibility. Notably, high-precision flexible patterning with a minimum feature size of 5.2 µm and spatial control over the polymerization of PPy is achieved. Moreover, PPy microwires are constructed into a photodetector that exhibits a responsivity of 644 A/W at 0.1-V bias under ultraviolet (UV) irradiation. Ultimately, an image sensor is fabricated by integrating multiple photodetectors, demonstrating the application potential of FsLDW technology for developing miniaturized and integrated electronic devices based on conductive polymers.
Sub-wavelength tunneling barrier in rib waveguide microring modulators with vanishing bending losses
Authors: Ackermann Manuel , Merget Florian , Witzens Jeremy
Published: April 2022

Silicon photonics ring resonators in rib waveguide configuration are among the most important components for wavelength-division-multiplexed communication networks. While the rib waveguide enables simple electrical connectivity in microring modulators and add-drop multiplexers, it also results in unacceptable bending losses once the circumference is shrunk below a few micrometers, limiting achievable free spectral ranges and resonant enhancements. We introduce a sub-wavelength tunneling barrier at the critical radius at which the conformally mapped effective index of the slab exceeds that of the waveguide in order to suppress these bending losses, while increasing the resonator’s resistance only slightly. The fundamental working principle is explained and illustrated with a design study based on the finite difference eigenmode method. Three-dimensional finite difference time domain simulations verify the design and a proof-of-concept microring modulator is modeled based on the novel geometry.
Context coding in the mouse nucleus accumbens modulates motivationally relevant information
Authors: Gmaz Jimmie M. , van der Meer Matthijs A. A.
Published: April 2022

Neural activity in the nucleus accumbens (NAc) is thought to track fundamentally value-centric quantities linked to reward and effort. However, the NAc also contributes to flexible behavior in ways that are difficult to explain based on value signals alone, raising the question of if and how nonvalue signals are encoded in NAc. We recorded NAc neural ensembles while head-fixed mice performed an odor-based biconditional discrimination task where an initial discrete cue modulated the behavioral significance of a subsequently presented reward-predictive cue. We extracted single-unit and population-level correlates related to the cues and found value-independent coding for the initial, context-setting cue. This context signal occupied a population-level coding space orthogonal to outcome-related representations and was predictive of subsequent behaviorally relevant responses to the reward-predictive cues. Together, these findings support a gating model for how the NAc contributes to behavioral flexibility and provide a novel population-level perspective from which to view NAc computations.
Population heterogeneity in clinical cohorts affects the predictive accuracy of brain imaging
Authors: Benkarim Oualid , Paquola Casey , Park Bo-yong , Kebets Valeria , Hong Seok-Jun , Vos de Wael Reinder , Zhang Shaoshi , Yeo B. T. Thomas , Eickenberg Michael , Ge Tian , Poline Jean-Baptiste , Bernhardt Boris C. , Bzdok Danilo
Published: April 2022

Brain imaging research enjoys increasing adoption of supervised machine learning for single-participant disease classification. Yet, the success of these algorithms likely depends on population diversity, including demographic differences and other factors that may be outside of primary scientific interest. Here, we capitalize on propensity scores as a composite confound index to quantify diversity due to major sources of population variation. We delineate the impact of population heterogeneity on the predictive accuracy and pattern stability in 2 separate clinical cohorts: the Autism Brain Imaging Data Exchange (ABIDE,n= 297) and the Healthy Brain Network (HBN,n= 551). Across various analysis scenarios, our results uncover the extent to which cross-validated prediction performances are interlocked with diversity. The instability of extracted brain patterns attributable to diversity is located preferentially in regions part of the default mode network. Collectively, our findings highlight the limitations of prevailing deconfounding practices in mitigating the full consequences of population diversity.
phastSim: Efficient simulation of sequence evolution for pandemic-scale datasets
Authors: De Maio Nicola , Boulton William , Weilguny Lukas , Walker Conor R. , Turakhia Yatish , Corbett-Detig Russell , Goldman Nick
Published: April 2022

Sequence simulators are fundamental tools in bioinformatics, as they allow us to test data processing and inference tools, and are an essential component of some inference methods. The ongoing surge in available sequence data is however testing the limits of our bioinformatics software. One example is the large number of SARS-CoV-2 genomes available, which are beyond the processing power of many methods, and simulating such large datasets is also proving difficult. Here, we present a new algorithm and software for efficiently simulating sequence evolution along extremely large trees (e.g. > 100, 000 tips) when the branches of the tree are short, as is typical in genomic epidemiology. Our algorithm is based on the Gillespie approach, and it implements an efficient multi-layered search tree structure that provides high computational efficiency by taking advantage of the fact that only a small proportion of the genome is likely to mutate at each branch of the considered phylogeny. Our open source software allows easy integration with other Python packages as well as a variety of evolutionary models, including indel models and new hypermutability models that we developed to more realistically represent SARS-CoV-2 genome evolution.
α-Phenylalanyl tRNA synthetase competes with Notch signaling through its N-terminal domain
Authors: Ho Manh Tin , Lu Jiongming , Vazquez-Pianzola Paula , Suter Beat
Published: April 2022

The alpha subunit of the cytoplasmic Phenylalanyl tRNA synthetase (α-PheRS, FARSA in humans) displays cell growth and proliferation activities and its elevated levels can induce cell fate changes and tumor-like phenotypes that are neither dependent on the canonical function of charging tRNAPhe with phenylalanine nor on stimulating general translation. In intestinal stem cells of Drosophila midguts, α-PheRS levels are naturally slightly elevated and human FARSA mRNA levels are elevated in multiple cancers. In the Drosophila midgut model, elevated α-PheRS levels caused the accumulation of many additional proliferating cells resembling intestinal stem cells (ISCs) and enteroblasts (EBs). This phenotype partially resembles the tumor-like phenotype described as Notch RNAi phenotype for the same cells. Genetic interactions between α-PheRS and Notch suggest that their activities neutralize each other and that elevated α-PheRS levels attenuate Notch signaling when Notch induces differentiation into enterocytes, type II neuroblast stem cell proliferation, or transcription of a Notch reporter. These non-canonical functions all map to the N-terminal part of α-PheRS which accumulates naturally in the intestine. This truncated version of α-PheRS (α-S) also localizes to nuclei and displays weak sequence similarity to the Notch intracellular domain (NICD), suggesting that α-S might compete with the NICD for binding to a common target. Supporting this hypothesis, the tryptophan (W) residue reported to be key for the interaction between the NICD and the Su(H) BTD domain is not only conserved in α-PheRS and α-S, but also essential for attenuating Notch signaling.
Impact of visual impairment on balance and visual processing functions in students with special educational needs
Authors: Choi Kai Yip , Wong Ho Yin , Cheung Hoi Nga , Tseng Jung Kai , Chen Ching Chung , Wu Chieh Lin , Eng Helen , Woo George C. , Cheong Allen Ming Yan
Published: April 2022

Introduction

Vision is critical for children’s development. However, prevalence of visual impairment (VI) is high in students with special educational needs (SEN). Other than VI, SEN students are prone to having functional deficits. Whether visual problems relate to these functional deficits is unclear. This study aimed to assess the impact of vision on visual processing functions and balance in SEN students through a community service.

Methods

Visual acuity (VA) and contrast sensitivity were measured in a total of 104 (aged 14.3±4.3) SEN students as the visual outcomes, followed by retinoscopy. Visual processing function assessment included facial expression recognition by card matching examiner’s facial expression matching, and visual orientation recognition. Dynamic balance, by Timed Up and Go test, and static standing balance (postural sway in double-legged standing with feet-together and tandem-stance for open-eye and closed-eye conditions) were assessed. Static balance was presented in terms of the maximal medial-lateral and antero-posterior sways.

Results

Of the 104 students, 62 (59.6%) were classified as visually impaired according to WHO classification of visual impairment based on presenting distance acuity. Ocular problems (e.g. optic nerve anomaly, uncorrected/ under-corrected refractive errors) and neurological anomalies were the major causes of vision loss. VA was positively associated with visual processing functions (all p ≤ 0.01), as SEN students with better vision tended to perform better in visual orientation and facial expression recognition tasks, as well as dynamic balance function (p = 0.04). For the static balance, postural sway and VA showed a positive relationship under open-eye and tandem stance conditions. However, the relationship between postural sway and VA became negative under closed-eye and tandem stance conditions.

Conclusion

This study found a high prevalence of SEN students with visual impairment, in which many of them were undetected. Optometric examination is important to improve their visual function to minimize the effect of vision on functional performance. Vision is critical in visual processing as well as playing an important role in maintaining balance in SEN students.
A polypeptide model for toxic aberrant proteins induced by aminoglycoside antibiotics
Authors: Tawde Mangala , Bior Abdelaziz , Feiss Michael , Teng Feiyue , Freimuth Paul
Published: April 2022

Aminoglycoside antibiotics interfere with the selection of cognate tRNAs during translation, resulting in the synthesis of aberrant proteins that are the ultimate cause of cell death. However, the toxic potential of aberrant proteins and how they avoid degradation by the cell’s protein quality control (QC) machinery are not understood. Here we report that levels of the heat shock (HS) transcription factor σ32 increased sharply following exposure of Escherichia coli to the aminoglycoside kanamycin (Kan), suggesting that at least some of the aberrant proteins synthesized in these cells were recognized as substrates by DnaK, a molecular chaperone that regulates the HS response, the major protein QC pathway in bacteria. To further investigate aberrant protein toxic potential and interaction with cell QC factors, we studied an acutely toxic 48-residue polypeptide (ARF48) that is encoded by an alternate reading frame in a plant cDNA. As occurred in cells exposed to Kan, σ32 levels were strongly elevated following ARF48 expression, suggesting that ARF48 was recognized as a substrate by DnaK. Paradoxically, an internal 10-residue region that was tightly bound by DnaK in vitro also was required for the ARF48 toxic effect. Despite the increased levels of σ32, levels of several HS proteins were unchanged following ARF48 expression, suggesting that the HS response had been aborted. Nucleoids were condensed and cell permeability increased rapidly following ARF48 expression, together suggesting that ARF48 disrupts DNA-membrane interactions that could be required for efficient gene expression. Our results are consistent with earlier studies showing that aberrant proteins induced by aminoglycoside antibiotics disrupt cell membrane integrity. Insights into the mechanism for this effect could be gained by further study of the ARF48 model system.
Comparative analysis of cardio-cerebrovascular complications in immigrants and native-born Koreans with diabetes: Risk factors and perspectives
Authors: Cho Hyemin , Jeoung Sohyun , Kang Cinoo , Jang Sunmee
Published: April 2022

Background

Given the rapidly increasing number of immigrants, it is crucial to address health care issues involving immigrants to facilitate their safe and secure settlement. Especially for common chronic diseases, such as diabetes, immigrants face more complex obstacles to manage their chronic conditions than do native-born residents. Therefore, we aimed to assess differences in the incidence and associated risk factors of cardio-cerebrovascular (CCV) complications of immigrants compared with native-born Koreans with diabetes.

Methods

Immigrants and native-born Koreans who had new diagnosis of diabetes and simultaneously received anti-diabetic prescriptions in 2012 were defined by using Korean National Health Insurance Claim Database(KNHICD). CCV complications were assessed at a 3-year follow-up from the index date. We assessed differences in the CCV complications and risk factors using multiple cox regression models.

Results

In total, 4,008 patients (668 of immigrants and 3,340 of native-born Koreans) who had newly diagnosed diabetes and simultaneously received anti-diabetic prescriptions in 2012 were selected. Immigrants with diabetes were at a 1.39 times higher risk of having CCV complications than native-born Koreans with diabetes (95% CI: 1.021–1.881). Patients who had a usual sources of care (USC) presented a significantly reduced risk of cardio-cerebrovascular complication (HR: 0.452; 95% CI: 0.342–0.598) in both immigrants and native Koreans. In subgroup analysis in immigrants, patients having USC showed decreased risk of CCV incidence (HR: 0.35, 95% CI: 0.175–0.703), whereas >60 years old and Charlson comorbidity index (CCI) score >1 presented increased risk of CCV complications.

Conclusion

Immigrants with diabetes have a higher risk of CCV complications than native-born Koreans with diabetes. However, having a USC significantly decreased the risk of CCV complications. Therefore, the utilization of USC will benefit to reduce diabetic complications in immigrants as well as reduction of overall health care cost burden, it would be necessary to implement USC in diabetes care at the initial disease stage.
Landscape diversity and local temperature, but not climate, affect arthropod predation among habitat types
Authors: Fricke Ute , Steffan-Dewenter Ingolf , Zhang Jie , Tobisch Cynthia , Rojas-Botero Sandra , Benjamin Caryl S. , Englmeier Jana , Ganuza Cristina , Haensel Maria , Riebl Rebekka , Uhler Johannes , Uphus Lars , Ewald Jörg , Kollmann Johannes , Redlich Sarah
Published: April 2022

Arthropod predators are important for ecosystem functioning by providing top-down regulation of insect herbivores. As predator communities and activity are influenced by biotic and abiotic factors on different spatial scales, the strength of top-down regulation (‘arthropod predation’) is also likely to vary. Understanding the combined effects of potential drivers on arthropod predation is urgently needed with regard to anthropogenic climate and land-use change. In a large-scale study, we recorded arthropod predation rates using artificial caterpillars on 113 plots of open herbaceous vegetation embedded in contrasting habitat types (forest, grassland, arable field, settlement) along climate and land-use gradients in Bavaria, Germany. As potential drivers we included habitat characteristics (habitat type, plant species richness, local mean temperature and mean relative humidity during artificial caterpillar exposure), landscape diversity (0.5–3.0-km, six scales), climate (multi-annual mean temperature, ‘MAT’) and interactive effects of habitat type with other drivers. We observed no substantial differences in arthropod predation rates between the studied habitat types, related to plant species richness and across the Bavarian-wide climatic gradient, but predation was limited when local mean temperatures were low and tended to decrease towards higher relative humidity. Arthropod predation rates increased towards more diverse landscapes at a 2-km scale. Interactive effects of habitat type with local weather conditions, plant species richness, landscape diversity and MAT were not observed. We conclude that landscape diversity favours high arthropod predation rates in open herbaceous vegetation independent of the dominant habitat in the vicinity. This finding may be harnessed to improve top-down control of herbivores, e.g. agricultural pests, but further research is needed for more specific recommendations on landscape management. The absence of MAT effects suggests that high predation rates may occur independent of moderate increases of MAT in the near future.
Identifying monitoring information needs that support the management of fish in large rivers
Authors: Counihan Timothy D. , Bouska Kristen L. , Brewer Shannon K. , Jacobson Robert B. , Casper Andrew F. , Chapman Colin G. , Waite Ian R. , Sheehan Kenneth R. , Pyron Mark , Irwin Elise R. , Riva-Murray Karen , McKerrow Alexa J. , Bayer Jennifer M.
Published: April 2022

Management actions intended to benefit fish in large rivers can directly or indirectly affect multiple ecosystem components. Without consideration of the effects of management on non-target ecosystem components, unintended consequences may limit management efficacy. Monitoring can help clarify the effects of management actions, including on non-target ecosystem components, but only if data are collected to characterize key ecosystem processes that could affect the outcome. Scientists from across the U.S. convened to develop a conceptual model that would help identify monitoring information needed to better understand how natural and anthropogenic factors affect large river fishes. We applied the conceptual model to case studies in four large U.S. rivers. The application of the conceptual model indicates the model is flexible and relevant to large rivers in different geographic settings and with different management challenges. By visualizing how natural and anthropogenic drivers directly or indirectly affect cascading ecosystem tiers, our model identified critical information gaps and uncertainties that, if resolved, could inform how to best meet management objectives. Despite large differences in the physical and ecological contexts of the river systems, the case studies also demonstrated substantial commonalities in the data needed to better understand how stressors affect fish in these systems. For example, in most systems information on river discharge and water temperature were needed and available. Conversely, information regarding trophic relationships and the habitat requirements of larval fishes were generally lacking. This result suggests that there is a need to better understand a set of common factors across large-river systems. We provide a stepwise procedure to facilitate the application of our conceptual model to other river systems and management goals.
Using machine learning for the personalised prediction of revision endoscopic sinus surgery
Authors: Nuutinen Mikko , Haukka Jari , Virkkula Paula , Torkki Paulus , Toppila-Salmi Sanna
Published: April 2022

Background

Revision endoscopic sinus surgery (ESS) is often considered for chronic rhinosinusitis (CRS) if maximal conservative treatment and baseline ESS prove insufficient. Emerging research outlines the risk factors of revision ESS. However, accurately predicting revision ESS at the individual level remains uncertain. This study aims to examine the prediction accuracy of revision ESS and to identify the effects of risk factors at the individual level.

Methods

We collected demographic and clinical variables from the electronic health records of 767 surgical CRS patients ≥16 years of age. Revision ESS was performed on 111 (14.5%) patients. The prediction accuracy of revision ESS was examined by training and validating different machine learning models, while the effects of variables were analysed using the Shapley values and partial dependence plots.

Results

The logistic regression, gradient boosting and random forest classifiers performed similarly in predicting revision ESS. Area under the receiving operating characteristic curve (AUROC) values were 0.744, 0.741 and 0.730, respectively, using data collected from the baseline visit until six months after baseline ESS. The length of time during which data were collected improved the prediction performance. For data collection times of 0, 3, 6 and 12 months after baseline ESS, AUROC values for the logistic regression were 0.682, 0.715, 0.744 and 0.784, respectively. The number of visits before or after baseline ESS, the number of days from the baseline visit to the baseline ESS, patient age, CRS with nasal polyps (CRSwNP), asthma, non-steroidal anti-inflammatory drug exacerbated respiratory disease and immunodeficiency or suspicion of it all associated with revision ESS. Patient age and number of visits before baseline ESS carried non-linear effects for predictions.

Conclusions

Intelligent data analysis identified important predictors of revision ESS at the individual level, such as the frequency of clinical visits, patient age, Type 2 high diseases and immunodeficiency or a suspicion of it.
Propensity or diversity? Investigating how mind wandering influences the incubation effect of creativity
Authors: Teng Shan-Chuan , Lien Yunn-Wen
Published: April 2022

Mind wandering has been argued to be beneficial for breaking through mental impasses, which leads to better creative performance upon a second attempt (i.e., the incubation effect). However, the evidence is inconsistent. Different from the propensity for mind wandering that has been the focus of past studies, in this study we further examined the role of diversity (i.e., non-repetitiveness of mind wandering respective to its content) and types of mind wandering along the dimensions of intentionality and awareness during incubation when engaging in a 0-back task (a mind wandering-prone condition) and a focused-breathing practice (a mindfulness-induced condition). We proposed that diversity rather than the propensity for mind wandering was crucial for post-incubation divergent creativity and that mindfulness induction would be a more effective way to elicit the incubation effect because it should result in fewer but more diverse mind-wandering incidents than engaging in a mind wandering-prone task. We conducted an experiment with a between-participant variable (incubation tasks: mind wandering-prone, mindfulness-induced, and no incubation). As predicted, the mindfulness-induced group (N = 30) outperformed the control group (N = 31) on flexibility for the unusual uses task measuring divergent thinking after incubation, but the mind wandering-prone group (N = 29) did not outperform the control group. In addition, the diversity of mind wandering and the tendency toward intentional mind wandering predicted the magnitude of incubation effects on flexibility and originality, respectively. Theoretical and practical implications are discussed.
Self-perceived job insecurity and self-reported health: Differences between native-born and migrant workers based on evidence from the Sixth European Working Conditions Survey
Authors: Nappo Nunzia
Published: April 2022

This paper analyses the association between self-perceived job insecurity and self-reported health by comparing two population groups, native-born and migrant workers, in EU15 countries. The econometric analysis employs data from the Sixth European Working Conditions Survey that was released in 2017. The health outcome examined in this study is self-reported health, which is a subjective indicator. Self-perceived job insecurity is an individual’s subjective evaluation of the possibility of future job loss. The association between job insecurity and self-reported health was tested using standard probit models and standard ordered probit models, considering the entire population sample, only native-born workers, only migrant workers. The results show that workers who think that they might lose their jobs have a lower probability of reporting very good and good health than workers who do not worry about losing their jobs, with job insecurity reducing the probability of reporting good health more for migrant workers than for native-born workers.
Transcriptomic analysis of chloride tolerance in Leptospirillum ferriphilum DSM 14647 adapted to NaCl
Authors: Rivera-Araya Javier , Heine Thomas , Chávez Renato , Schlömann Michael , Levicán Gloria
Published: April 2022

Chloride ions are toxic for most acidophilic microorganisms. In this study, the chloride tolerance mechanisms in the acidophilic iron-oxidizing bacteriumLeptospirillum ferriphilumDSM 14647 adapted to 180 mM NaCl were investigated by a transcriptomic approach. Results showed that 99 genes were differentially expressed in the adapted versus the non-adapted cultures, of which 69 and 30 were significantly up-regulated or down-regulated, respectively. Genes that were up-regulated include carbonic anhydrase, cytochromecoxidase (ccoN) and sulfide:quinone reductase (sqr), likely involved in intracellular pH regulation. Towards the same end, the cation/proton antiporter CzcA (czcA) was down-regulated. Adapted cells showed a higher oxygen consumption rate (2.2 x 10−9ppm O2s-1cell-1) than non-adapted cells (1.2 x 10−9ppm O2s-1cell-1). Genes coding for the antioxidants flavohemoprotein and cytochromecperoxidase were also up-regulated. Measurements of the intracellular reactive oxygen species (ROS) level revealed that adapted cells had a lower level than non-adapted cells, suggesting that detoxification of ROS could be an important strategy to withstand NaCl. In addition, data analysis revealed the up-regulation of genes for Fe-S cluster biosynthesis (iscR), metal reduction (merA) and activation of a cellular response mediated by diffusible signal factors (DSFs) and the second messenger c-di-GMP. Several genes related to the synthesis of lipopolysaccharide and peptidoglycan were consistently down-regulated. Unexpectedly, the genesectB,ectCandectDinvolved in the biosynthesis of the compatible solutes (hydroxy)ectoine were also down-regulated. In line with these findings, although hydroxyectoine reached 20 nmol mg-1of wet biomass in non-adapted cells, it was not detected inL.ferriphilumadapted to NaCl, suggesting that this canonical osmotic stress response was dispensable for salt adaptation. Differentially expressed transcripts and experimental validations suggest that adaptation to chloride in acidophilic microorganisms involves a multifactorial response that is different from the response in other bacteria studied.
Determinants for late presentation of glaucoma among adult glaucomatous patients in University of Gondar Comprehensive Specialized Hospital. Case-control study
Authors: Belete Biruktayit Kefyalew , Assefa Natnael Lakachew , Assem Abel Sinshaw , Ayele Fisseha Admasu
Published: April 2022

Introduction

Glaucoma is a disease which causes optic nerve damage and remains a major public health concern worldwide. Late presentation is a major risk factor for glaucoma induced blindness. The aim of this study was to assess determinants for late presentation of glaucoma among adult glaucomatous patients.

Methods

A hospital-based case-control study was conducted among 452 adult glaucomatous patients. Late presenters were glaucoma patients diagnosed with cup to disc ratio (CDR) > 0.8 and mean deviation of greater than -14 decibel in either of the eyes at their first presentation. Study participants were selected among glaucomatous follow-up patients by using systematic random sampling. Data were entered into EPI Info version 7 and exported to SPSS version 22 for analysis. Bivariable and multivariable logistic regression analysis was done to identify determinants. Variables with P-value < 0.05 were considered as statistically significant.

Result

The mean age of participants were 55.1 ± 13.2 years. Being > 60 years of age, 4.51 times (AOR: 4.51; 95% CI: 1.74, 11.67), resided > 53 km away from the hospital 6.02 times (AOR: 6.02; 2.76, 13.14), Presenting IOP > 30 mmHg, 4.49 times (AOR: 4.49, 95% CI: 2.10, 9.12), poor knowledge of glaucoma, 4.46 times (AOR: 4.46, CI: 2.62, 7.58) and absence of regular eye checkup, 2.35 times (AOR: 2.35, 95% CI: 1.09, 5.47) higher odds of being late presenter.

Conclusion

Increasing age, high IOP, poor knowledge of glaucoma, residing far away from the hospital and absence of regular eye checkups were significantly associated with late presentation.
Non-polyalanine repeat mutation in PHOX2B is detected in autopsy cases of sudden unexpected infant death
Authors: Ueda Atsushi , Osawa Motoki , Naito Haruaki , Ochiai Eriko , Kakimoto Yu
Published: April 2022

Background

Congenital central hypoventilation syndrome (CCHS), which is caused by PHOX2B with phenotypic variations, has a point of controversy: CCHS is putatively involved in autopsy cases of sudden unexpected infant death (SUID) including sudden infant death syndrome.

Objective

The relation of CCHS to SUID cases was investigated by extensive genotyping of PHOX2B.

Methods

We analyzed 93 DNA samples of less than one-year-old SUID cases that were autopsied in our department. Unrelated adult volunteers (n = 942) were used as the control.

Results

No polyalanine tract expansion was detected in the SUID cases. The allelic frequencies of repeat contractions and SNP (rs28647582) in intron 2 were not significantly different from that in those control group. Further extensive sequencing revealed a non-polyalanine repeat mutation (NPARM) of c.905A>C in a sudden death case of a one-month-old male infant. This missense mutation (p.Asn302Thr), registered as rs779068107, was annotated to ‘Affected status is unknown’, but it might be associated with the sudden death.

Conclusion

NPARM was more plausibly related to sudden unexpected death than expansions because of severe clinical complications. This finding indicates possible CCHS involvement in forensic autopsy cases without ante-mortem diagnosis.
AT2R activation increases in vitro angiogenesis in pregnant human uterine artery endothelial cells
Authors: Mishra Jay S. , Chen Dong-Bao , Kumar Sathish
Published: April 2022

Angiogenesis is vital during pregnancy for remodeling and enhancing vasodilation of maternal uterine arteries, and increasing uterine blood flow. Abnormal angiogenesis is associated with decreased uteroplacental blood flow and development of pregnancy disorders such as gestational hypertension, preeclampsia, fetal growth restriction, preterm delivery, stillbirth, and miscarriage. The mechanisms that contribute to normal angiogenesis remain obscure. Our previous studies demonstrated that expression of the angiotensin type 2 receptor (AT2R) is increased while the angiotensin type 1 receptor (AT1R) is unchanged in the endothelium of uterine arteries, and that AT2R-mediated pregnancy adaptation facilitates enhanced vasodilation and uterine arterial blood flow. However, the role of AT2R in regulating angiogenesis during pregnancy has never been studied. This study examines whether or not AT2R activation induces angiogenesis and, if so, what mechanisms are involved. To this end, we used primary human uterine artery endothelial cells (hUAECs) isolated from pregnant and nonpregnant women undergoing hysterectomy. The present study shows that Compound 21, a selective AT2R agonist, induced proliferation of pregnant-hUAECs, but not nonpregnant-hUAECs, in a concentration-dependent manner, and that this C21-induced mitogenic effect was blocked by PD123319, a selective AT2R antagonist. The mitogenic effects induced by C21 were inhibited by blocking JNK—but not ERK, PI3K, and p38—signaling pathways. In addition, C21 concentration dependently increased cell migration and capillary-like tube formation in pregnant-hUAECs. The membrane-based antibody array showed that C21 increased expression of multiple angiogenic proteins, including EGF, bFGF, leptin, PLGF, IGF-1, and angiopoietins. Our qPCR analysis demonstrates that C21-induced increase in expression of these angiogenic proteins correlates with a proportional increase in mRNA expression, indicating that AT2R activates angiogenic proteins at the transcriptional level. In summary, the present study shows that AT2R activation induces angiogenesis of hUAECs in a pregnancy-specific manner through JNK-mediated pathways with associated transcriptional upregulation of multiple proangiogenic proteins.
Complex pain phenotypes: Suicidal ideation and attempt through latent multimorbidity
Authors: Song Kangwon , Brintz Ben J. , Wang Chen-Pin , McGeary Donald D. , McGeary Cindy A. , Potter Jennifer S. , Jaramillo Carlos A. , Eapen Blessen C. , Pugh Mary Jo
Published: April 2022

Background

Given the relatively high rates of suicidal ideation and attempt among people with chronic pain, there is a need to understand the underlying factors to target suicide prevention efforts. To date, no study has examined the association between pain phenotypes and suicide related behaviors among those with mild traumatic brain injuries.

Objective

To determine if pain phenotypes were independently associated with suicidal ideation / attempt or if comorbidities within the pain phenotypes account for the association between pain phenotypes and suicide related behaviors.

Methods

This is a longitudinal retrospective cohort study of suicide ideation/attempts among pain phenotypes previously derived using general mixture latent variable models of the joint distribution of repeated measures of pain scores and pain medications/treatment. We used national VA inpatient, outpatient, and pharmacy data files for Post-9/11 Veterans with mild traumatic injury who entered VA care between fiscal years (FY) 2007 and 2009. We considered a counterfactual causal modeling framework to assess the extent that the pain phenotypes during years 1–5 of VA care were predictive of suicide ideation/attempt during years 6–8 of VA care conditioned on covariates being balanced between pain phenotypes.

Results

Without adjustment, pain phenotypes were significant predictors of suicide related behaviors. When we used propensity scores to balance the comorbidities present in the pain phenotypes, the pain phenotypes were no longer significantly associated with suicide related behaviors.

Conclusion

These findings suggest that suicide ideation/attempt is associated with pain trajectories primarily through latent multimorbidity. Therefore, it is critical to identify and manage comorbidities (e.g., depression, post-traumatic stress disorder) to prevent tragic outcomes associated with suicide related behaviors throughout the course of chronic pain and mild traumatic brain injury management.
Experiencias de cuidado y configuraciones subjetivas de madres de niños y niñas con discapacidad
Authors: Díaz Alzate Magda Victoria , Ramírez Taborda Mónica Melissa
Published: April 2022

El cuidado de niños y niñas con discapacidad ha sido un encargo a las mujeres madres en las diferentes sociedades, lo que implica sentimientos de culpa y sometimiento que afectan sus configuraciones subjetivas, anclándolas a las consideraciones patriarcales de lo que es ser buena madre. La investigación se preguntó por las experiencias de cuidado y las configuraciones subjetivas de mujeres madres de niños y niñas con discapacidad. A partir de la metodología de las Producciones Narrativas, se lograron develar dos grandes hallazgos: las experiencias de cuidado que se construyen a partir de los diagnósticos, la discriminación y el cuidado desde la dimensión ética, y la configuración de subjetividades femeninas que están ancladas a la naturalización del patriarcado, la renuncia y el sacrificio. Se concluye que es importante reflexionar sobre el cuidado como labor exclusiva de las mujeres, especialmente de personas con discapacidad, a partir de propuestas como la coeducación, que no solo tendría en cuenta la equidad de género en asuntos de cuidado, sino también la reflexión educativa a favor del reconocimiento de la diversidad humana.
Being Interdisciplinary: Adventures in urban science and beyond
Authors: Wilson Alan
Published: April 2022

In Being Interdisciplinary, Alan Wilson draws on five decades as a leading figure in urban science to set out a systems approach to interdisciplinarity for those conducting research in this and other fields. He argues that most research is interdisciplinary at base, and that a systems perspective is particularly appropriate for collaboration because it fosters an outlook that sees beyond disciplines. There is a more subtle thread, too. A systems approach enables researchers to identify the game-changers of the past as a basis for thinking outside convention, for learning how to do something new and how to be ambitious, in a nutshell how to be creative. Ultimately, the ideas presented address how to do research. Building on this systems focus, the book first establishes the basics of interdisciplinarity. Then, by drawing on the author’s experience of doing interdisciplinary research, and working from his personal toolkit, it offers general principles and a framework from which researchers can build their own interdisciplinary toolkit, with elements ranging from explorations of game-changers in research to superconcepts. In the last section, the book tackles questions of managing and organising research from individual to institutional scales. Alan Wilson deploys his wide experience – researcher in urban science, university professor and vice-chancellor, civil servant and institute director – to build the narrative. While his experience in urban science provides the illustrations, the principles apply across many research fields.
The Things That Really Matter: Philosophical conversations on the cornerstones of life
Authors: Hauskeller Michael
Published: April 2022

While being rooted in the academic discourse, The Things That Really Matter comprehensively explores the most fundamental aspects of human life in an accessible, non-technical language, adding fresh perspectives and new arguments and considerations that are designed to stimulate further debate and, in some cases, a deliberate redirection of research interests in the respective areas. It features a series of conversations about the things in our life that we all, in one way or another, wrestle with if we are at all concerned about what kind of world we live in and what our role in it is: things like birth, age, and death, good and evil, the meaning of life, the nature of the self and the role the body plays for our identity, our gendered existence, love and faith, free will, beauty, and our experience of the sacred. Situating abstract ideas in concrete experience, The Things That Really Matter encourages the reader to participate in an open-ended dialogue involving a variety of thinkers with different backgrounds and orientations. Lively and accessible, it shows thinking as an open-ended process and a collaborative endeavour that benefits from talking to each other rather than against each other, featuring real conversations, where ideas are explored, tested, changed, and occasionally dropped. It is thinking in motion, personal yet universal.
Teaching and learning the legacy of residential schools for remembering and reconciliation in Canada
Authors: Wallace-Casey Cynthia
Published: April 2022

In 2015, the Truth and Reconciliation Commission (TRC) of Canada released a Final Report containing 94 Calls to Action. Included were calls for reform in how history is taught in Canadian schools, so that students may learn to address such difficult topics in Canadian history as Indian Residential Schools, racism and cultural genocide. Operating somewhat in parallel to these reforms, social studies curricula across Canada have undergone substantial revisions. As a result, historical thinking is now firmly embedded within the curricula of most provinces and territories. Coupled with these developments are various academic debates regarding public pedagogy, difficult knowledge and student beliefs about Canada’s colonial past. Such debates require that researchers develop a better understanding of how knowledge related to Truth and Reconciliation is currently presented within Canadian classrooms, and how this may (or may not) relate to historical thinking. In this paper, I explore this debate as it relates to Indian Residential Schools. I then analyse a selection of classroom resources currently available in Canada for teaching about Truth and Reconciliation. In so doing, I consider how these relate to Peter Seixas’s six concepts of historical thinking (Seixas and Morton, 2013), as well as broader discussions within Canada about Indigenous world views, historical empathy and Reconciliation.
Citizenship, Belonging and Crisis-Induced Returns of Ghanaian Migrants from Cote d’Ivoire
Authors:   University of Ghana, Kandilige Leander , Yeboah Thomas ,   Kwame Nkrumah University of Science and Technology, Kumasi Ghana, Kwabena Abutima Theophilus ,   University of Ghana
Published: April 2022

The socio-economic embeddedness of ECOWAS foreign nationals and migrants in Côte d’Ivoire has been amply described. Despite previous episodes of political violence and the somewhat recent anti-immigrant policies, Côte d’Ivoire has served and will continue to serve as a haven for foreign-born populations of ECOWAS nationals and Ivorian-born descendants of foreigners. This paper draws on the notion of citizenship and belonging, and qualitative data gathered through interviews to explore the experiences of returned Ghanaian migrants who were victimised and trapped in a conflict situation in Cote D’Ivoire in 2002-2003 and 2010-2011. The findings show that despite playing a passive role as perpetrators of the violence, Ghanaian migrants experienced harassment, intimidation and physical attacks which in turn contributed to traumatic feelings beyond crisis period. While the notion of citizenship and belonging facilitated migrants’ easy entry to Ghana upon return, they had to navigate multiple challenges including hostile attitudes of militants, rebels and vigilante groups at border checkpoints, extortion of cash and other material possessions. Reintegration and resettlement were further exacerbated due to their experiences. These findings have important implications for emergency responses to situations of migrants being caught up in crisis.
Los últimos días del gobierno español en Colombia
Authors: Sourdis Nájera Adelaida
Published: April 2022

Con la derrota de los españoles en Boyacá, el 7 de agosto de 1819, cayó Santafé la capital del virreinato de Nueva Granada en manos de los patriotas colombianos, pero no así el Estado español. El virrey Sámano y la Real Audiencia huyeron a refugiarse en Cartagena de Indias en donde continuaron gobernando. El conflicto entre Sámano, partidario del absolutismo monárquico, y Gabriel de Torres, gobernador de Cartagena, de ideas liberales, pronto se manifestó. Al negarse el virrey a jurar la Constitución de Cádiz restablecida en España en 1820, el gobernador, con fundamento en dicha carta, lo depuso y asumió el poder político y militar. Sámano se embarcó hacia Cuba y Torres quedó como único y legítimo Jefe del Estado español en lo que quedaba de la Nueva Granada. Defendió valientemente esta postrera posesión de la monarquía hasta que, asediado por el ejército colombiano comandado por Mariano Montilla y la armada por José Padilla, no tuvo más remedio que rendirse y entregar la Plaza Fuerte a los colombianos el 10 de octubre de 1821. Se embarcó con sus tropas hacia Cuba para nunca más volver.