bluuebunny/crossref_metadata_2025 huggingface dataset:
Production of Protein Concentrates from Macauba (Acrocomia aculeata and Acrocomia totai) Kernels by Sieve Fractionation
Authors: Toledo e Silva Sérgio Henrique , Silva Lidiane Bataglia , Eisner Peter , Bader-Mittermaier Stephanie
Published: November 2022

Macauba palm fruits (Acrocomia aculeata and Acrocomia totai) are emerging as sources of high-quality oils from their pulp and kernels. The protein-rich macauba kernel meal (MKM) left after oil extraction remains undervalued, mainly due to the lack of suitable deoiling parameters and integrated protein recovery methods. Therefore, the present study aimed to produce protein concentrates from MKM using sieve fractionation. The deoiling parameters, comprising pressing, milling, and solvent extraction, were improved in terms of MKM functionality. The combination of hydraulic pressing, milling to 1 mm, and the hexane extraction of A. aculeata kernels resulted in MKM with the highest protein solubility (77.1%), emulsifying activity index (181 m2/g protein), and emulsion stability (149 min). After sieve fractionation (cut size of 62 µm), this meal yielded a protein concentrate with a protein content of 65.6%, representing a 74.1% protein enrichment compared to the initial MKM. This protein concentrate showed a reduced gelling concentration from 8 to 6%, and an increased emulsion stability from 149 to 345 min, in comparison to the MKM before sieving. Therefore, sieve fractionation after improved deoiling allows for the simple, cheap, and environmentally friendly recovery of MKM proteins, highlighting the potential of macauba kernels as a new source of protein.
Twin-Screw Extrusion as Hydrothermal Technology for the Development of Gluten-Free Teff Flours: Effect on Antioxidant, Glycaemic Index and Techno-Functional Properties
Authors: Martín-Diana Ana Belén , Blanco Espeso Belén , Jimenez Pulido Ivan Jesus , Acebes Martínez Pedro J. , Rico Daniel
Published: November 2022

Gluten-free products (GFP) currently are the fastest-growing category of baked goods probably due to the high worldwide incidence of celiac disease (CD). Refined rice is one of the most used cereal flour for GFP development, due to its high content in starch and good technological aptitude. However, its low content in fibre, protein and minerals has awakened a recent interest as alternative to balance the GF flour formulas. Teff is a cereal with high levels in fibre and antioxidants compounds but the lack of gluten results in very limited techno-functional properties. Extrusion is a thermal technology that allows to combine flours, overcoming negative impacts on quality characteristics. This study evaluated the effect of twin-screw extruder on rice-teff (white and brown) mixtures with different teff concentrations (25, 50 and 75%) on their antioxidant, glycaemic index and techno-functional properties. The results showed than the high shear–temperature process produced important modifications on the flour, which were confirmed using scanning electron microscopy (SEM). Significant increases in total dietary fibre (16 to 100% increase) were observed in teff containing flours, due to carbohydrate–lipid–protein complexes, which lead to resistant starch, with no significant increase in rice flour. Hydration and pasting properties were significantly (p > 0.05) affected by extrusion, and the effect was related to the concentration of teff used. The thermal process showed a decrease in total phenol (TP) content for rice; however, extrusion enhanced the release of total phenol in rice-teff blends, which was reflected on the antioxidant activities of blend flours, especially those prepared with brown teff.
Quantitative Acetylome Analysis of Soft Wheat Seeds during Artificial Ageing
Authors: Liang Liuke , Xie Aowen , Yang Haojie , Li Na , Ma Ping’an , Wei Shan , Zhang Shuaibing , Lv Yangyong , Hu Yuansen
Published: November 2022

Lysine acetylation (Kac) is a protein post-translational modification (PTM) widely found in plants that plays vital roles in metabolic pathways. Although seed germination and development are regulated by Kac, its potential function in seed ageing remains to be investigated. Our preliminary study demonstrated that Kac levels were altered during wheat seed artificial ageing. However, its specific role in this process still needs to be elucidated. Here, we performed quantitative acetylation proteomics analysis of soft wheat seeds with different germination rates during artificial ageing. A total of 175 acetylation proteins and 255 acetylation modification sites were remarkably changed. The differentially acetylated proteins were enriched in metabolism; response to harsh intracellular environment, such as ROS; protein storage and processing. Notably, expression, point mutation to mimic Kac by K to Q mutation at K80 and K138, protein purification and enzyme activity detection revealed that the Kac of ROS-scavenging glutathione transferase attenuated its activity, indicating that the defense ability of wheat seeds to stress gradually diminished, and the ageing process was inevitable. Collectively, our data provide a basis for further understanding the roles of Kac in seed ageing and might aid in the development of new techniques to prolong seed viability and food quality.
Sorption Isotherms, Glass Transition and Bioactive Compounds of Ingredients Enriched with Soluble Fibre from Orange Pomace
Authors: Perez-Pirotto Claudia , Moraga Gemma , Hernando Isabel , Cozzano Sonia , Arcia Patricia
Published: November 2022

Citrus fruits are one of the main crops worldwide. Its industrialization, primarily juice production, produces large amounts of byproducts, composed of seeds and peels, that can be used to obtain new ingredients. In this study, sorption behaviour, glass transition, mechanical properties, colour and bioactives of four different soluble fibre-enriched powders obtained from orange pomace using green technologies were studied. Powders were equilibrated at water activities between 0.113 and 0.680 for fifteen weeks at 20 °C, and studies were performed to indicate the best storing conditions to ensure the glassy state of the amorphous matrix and higher bioactive stability. By combining the Gordon and Taylor model with the Henderson isotherm, the critical water activity and content for storage in a glassy state were determined. The ingredient obtained after extrusion + hot water is the most stable, which is also the one with the highest dietary fibre content. Powder obtained by jet cooking is the least stable, as it is not in a glassy state at any water activity at room temperature. To increase storage stability, these should be stored at refrigeration temperatures.
Evaluation of the Volatile Composition and Sensory Behavior of Habanero Pepper during Lactic Acid Fermentation by L. plantarum
Authors: López-Salas Diego , Oney-Montalvo Julio Enrique , Ramírez-Rivera Emmanuel , Ramírez-Sucre Manuel Octavio , Rodríguez-Buenfil Ingrid Mayanin
Published: November 2022

Habanero pepper is recognized for its appealing aroma and flavor. Lactic acid fermentation can improve these sensory properties, especially aroma, by the synthesis of volatile compounds, which might also increase the consumer preference. Thus, the aim of this research was to compare the volatile composition as well as different sensory parameters such as preference and emotions related to the lactic acid fermentation of Habanero pepper by two strains (wild and commercial) of Lactiplantibacillus plantarum. A multiple factor ANOVA was used to compare the volatile composition with different fermentation times and strains. The results demonstrated that the interaction between the strain and fermentation time had significant effects on the volatile compound production that includes 1-hexanol, cis-3-hexenyl hexanoate, linalool, and 3,3 dimethyl-1-hexanol while only time influenced the production of trans-2-hexen-1-al. The wild strain (WIL) at 48 h of fermentation produced the highest concentration of 3,3 dimethyl-1-hexanol and trans-2-hexen-1-al. On the other hand, the commercial strain (COM) presented the highest concentration of 1-hexanol and cis-3-hexenyl hexanoate with a 72 h fermentation. The most preferred sample was that fermented by WIL for 48 h for the attribute of odor, while for taste, the most preferred sample was that fermented for 72 h with COM.
Adsorption Behavior of the L-Theanine onto Cation Exchange Resin ZGSPC106Na and D001SD
Authors: Yang Yusi , Dong Zhanbo , Wang Yongkang , Xiao Fengyi , Yang Jinliang , Zhao Dong , Ye Jianhui , Zheng Xinqiang , Liang Yuerong , Lu Jianliang
Published: November 2022

Adsorption is an important technology for the separation of different tea components. The adsorption behavior of L-theanine onto adsorbents was comprehensively studied in this paper. Among tested adsorbents, cation exchange resin ZGSPC106Na and D001SD were suitable for separating L-theanine, PVPP and PA-6 for catechins and macroporous resin HPD-400 for caffeine. Adsorption of L-theanine onto the cation resins was significantly influenced by the acidity, contact time and temperature. The adsorption behavior could be described by the pseudo-second-order rate equation and fitted to Langmuir and Freundlich models. ZGSPC106Na exhibited higher adsorption capacity, while D001SD showed higher adsorption selectivity. These might be attributed to the distinctive structure of the two resins and different ionization of the adsorbates. A method for simultaneous preparation of decaffeinated polyphenols, caffeine-enriched extract and decaffeinated L-theanine was established through successive separation on the columns fulfilled with PA-6, HPD-400 and D001SD, respectively.
Tenebrio molitor Proteins-Derived DPP-4 Inhibitory Peptides: Preparation, Identification, and Molecular Binding Mechanism
Authors: Tan Jiao , Yang Jing , Zhou Xinyi , Hamdy Ahmed Mahmoud , Zhang Xilu , Suo Huayi , Zhang Yu , Li Ning , Song Jiajia
Published: November 2022

Inhibition of dipeptidyl peptidase-4 (DPP-4) is an effective way to control blood glucose in diabetic patients. Tenebrio (T.) molitor is an edible insect containing abundant protein. T. molitor protein-derived peptides can suppress the DPP-4 activity. However, the amino acid sequence and binding mechanism of these DPP-4 inhibitory peptides remain unclear. This study used the flavourzyme for T. molitor protein hydrolysis, identified the released peptides with DPP-4 inhibitory effect, and investigated the binding interactions of these peptides with DPP-4. The results showed that flavourzyme efficiently hydrolyzed the T. molitor protein, as demonstrated by the high degree of hydrolysis, disappearance of protein bands in SDS-PAGE, and changes to protein structure. The 4-h flavourzyme hydrolysates showed a good inhibitory effect on DPP-4 (IC50 value of 1.64 mg/mL). The fragment of 1000–3000 Da accounted for 10.39% of the total peptides, but showed the strongest inhibitory effect on DPP-4. The peptides LPDQWDWR and APPDGGFWEWGD were identified from this fraction, and their IC50 values against DPP-4 were 0.15 and 1.03 mg/mL, respectively. Molecular docking showed that these two peptides interacted with the DPP-4 active site via hydrogen bonding, hydrophobic interactions, salt bridge formation, π-cation interactions, and π-π stacking. Our findings indicated that T. molitor protein-derived peptides could be used as natural DPP-4 inhibitors.
Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids
Authors: Lin Xue , Song Zeming , Jiang Huanyuan , Hao Yaofei , Hu Xiaoping , Liu Sixin , Li Congfa
Published: November 2022

The uncontrolled natural pre-fermentation process of coconut water represents great hidden safety hazards, unstable production, and impact on the quality of nata de coco–the trade name of bacterial cellulose (BC) in food industry. In this study, BC production from Komagataeibacter nataicola Q2 was conducted in the media of coconut water (50%, v/v) pre-fermented by 11 coconut-sourced yeast strains in static. Results suggested that coconut water pre-fermented by different yeast strains had varied effects on the production of BC. Compared with the use of fresh coconut water, the use of coconut water pre-fermented by Saccharomyces cerevisiae SC7 increased the BC yield by 165%. Both natural pre-fermentation and SC7 pre-fermentation altered the concentrations of amino acids in fresh coconut water. The addition of selected amino acids aspartic acid, glutamic acid, serine, methionine, threonine, isoleucine, phenylalanine, and proline at different concentrations had varied effects on the production of BC. The yield of BC was the highest when adding 3.0% (w/v) methionine. Moreover, adding 3.0% methionine allowed the production of BC with larger loops of looser aggregated microfibers, increased the crystallinity of BC from 64.8% to 69.4%, but decreased the temperature of maximum weight loss rate, hardness, and adhesiveness from 223 °C, 8.68 kg, and 92.8 g.sec to 212 °C, 7.01 kg, and 58.5 g.sec, respectively, in the test condition.
A Comprehensive Investigation of Macro-Composition and Volatile Compounds in Spring-Picked and Autumn-Picked White Tea
Authors: Zhang Cheng , Zhou Chengzhe , Xu Kai , Tian Caiyun , Zhang Mengcong , Lu Li , Zhu Chen , Lai Zhongxiong , Guo Yuqiong
Published: November 2022

The flavour of white tea can be influenced by the season in which the fresh leaves are picked. In this study, the sensory evaluation results indicated that spring-picked white tea (SPWT) was stronger than autumn-picked white tea (APWT) in terms of the taste of umami, smoothness, astringency, and thickness as well as the aromas of flower and fresh. To explore key factors of sensory differences, a combination of biochemical composition determination, widely targeted volatilomics (WTV) analysis, multivariate statistical analysis, and odour activity value (OAV) analysis was employed. The phytochemical analysis showed that the free amino acid, tea polyphenol, and caffeine contents of SPWTs were significantly higher than those of APWTs, which may explain the higher umami, smoothness, thickness, and astringency scores of SPWTs than those of APWTs. The sabinene, (2E, 4E)-2, 4-octadienal, (-)-cis-rose oxide, caramel furanone, trans-rose oxide, and rose oxide contents were significantly higher in SPWTs than in APWTs, which may result in stronger flowery, fresh, and sweet aromas in SPWTs than in APWTs. Among these, (2E,4E)-2,4-octadienal and (-)-cis-rose oxide can be identified as key volatiles. This study provides an objective and accurate basis for classifying SPWTs and APWTs at the metabolite level.
Phytosterols and γ-Oryzanol as Cholesterol Solid Phase Modifiers during Digestion
Authors: Esperança Eduardo S. , Bonatto Mariane S. , Silva Karen C. G. , Shimamoto Gustavo G. , Tubino Matthieu , Costa Mariana C. , Rodrigues Christianne E. C. , Meirelles Antonio J. A. , Sato Ana C. K. , Maximo Guilherme J.
Published: November 2022

Literature reports that ingestion of phytosterols and γ-oryzanol contributes to cholesterol lowering. Despite in vivo observations, thermodynamic phase equilibria could explain phenomena occurring during digestion leading to such effects. To advance the observations made by previous literature, this study was aimed at describing the complete solid–liquid phase equilibrium diagrams of cholesterol + phytosterol and γ-oryzanol systems by DSC, evaluating them by powder X-ray, microscopy, and thermodynamic modeling. Additionally, this study evaluated the phenomena observed by an in vitro digestibility method. Results confirmed the formation of solid solution in the cholesterol + phytosterols system at any concentration and that cholesterol + γ-oryzanol mixtures formed stable liquid crystalline phases with a significant melting temperature depression. The in vitro protocol supported the idea that the same phenomena can occur during digestion in which mechanochemical forces were probably the mechanisms promoting cholesterol solid phase changes in the presence of such phytocompounds. In this case, these changes could alter cholesterol solubility and possibly its absorption in the gastrointestinal lumen.
New Zwitterionic Polymer as a Highly Effective Salt- and Calcium-Resistant Fluid Loss Reducer in Water-Based Drilling Fluids
Authors: Liu Luman , Sun Jinsheng , Wang Ren , Liu Fan , Gao Shifeng , Yang Jie , Ren Han , Qu Yuanzhi , Cheng Rongchao , Geng Yuan , Feng Zhenbo
Published: November 2022

To control the filtration loss of drilling fluids in salt–gypsum formations, a novel type of zwitterionic polymer gel (DNDAP) was synthesized by free radical polymerization, which was used as a salt- and calcium-resistant fluid loss reducer for water-based drilling fluids (WBDF). DNDAP was prepared with N, N-dimethylacrylamide (DMAA), N-vinylpyrrolidone (NVP), Diallyl dimethyl ammonium chloride (DMDAAC), 2-acrylamide-2-methylpropaneonic acid (AMPS), and isopentenol polyether (TPEG) as raw materials. Fourier transform infrared spectroscopy (FT-IR) and proton nuclear magnetic resonance (1H-NMR) were used to characterize the composition and structure of the DNDAP copolymer. The thermal stability of DNDAP was evaluated by the use of thermogravimetric analysis (TGA). WBDF with DNDAP was analyzed for zeta potential and particle size and the corresponding filter cake underwent energy dispersive spectrum (EDS) analysis and scanning electron microscope (SEM) analysis. The results showed that the thermal decomposition of DNDAP mainly occurred above 303 °C. DNDAP exhibits excellent rheological and filtration properties in water-based drilling fluids, even under high-temperature aging (up to 200 °C) and high salinity (20 wt% NaCl or 5 wt% CaCl2) environments. The strong adsorption effect of DNDAP makes the particle size of bentonite reasonably distributed to form a dense mud cake that reduces filtration losses.
Herbal Fennel Essential Oil Nanogel: Formulation, Characterization and Antibacterial Activity against Staphylococcus aureus
Authors: Alam Aftab , Foudah Ahmed I. , Salkini Mohammad Ayman , Raish Mohammad , Sawale Jyotiram
Published: November 2022

Antimicrobial resistance (AMR) is one of the greatest threats to humanity in the world. Antibiotic-resistant bacteria spread easily in communities and hospitals. Staphylococcus aureus (S. aureus) is a serious human infectious agent with threatening broad-spectrum resistance to many commonly used antibiotics. To prevent the spread of pathogenic microorganisms, alternative strategies based on nature have been developed. Essential oils (EOs) are derived from numerous plant parts and have been described as antibacterial agents against S. aureus. Fennel essential oils were selected as antibacterial agents encapsulated in nanoparticles of polylactic acid and glycolic acid (PLGA). The optimum size of the formulation after loading with the active ingredient was 123.19 ± 6.1595 nm with a zeta potential of 0.051 ± 0.002 (23 ± 1.15 mV). The results of the encapsulation efficiency analysis showed high encapsulation of EOs, i.e., 66.4 ± 3.127. To obtain promising carrier materials for the delivery of fennel EOs, they were incorporated in the form of nanogels. The newly developed fennel oils in PLGANPs nanogels have good drug release and MIC against S. aureus. These results indicate the potential of this novel delivery system for antimicrobial therapy.
Land Suitability Evaluation of Tea (Camellia sinensis L.) Plantation in Kallar Watershed of Nilgiri Bioreserve, India
Authors: Rahaman S. Abdul , Aruchamy S.
Published: November 2022

Nilgiri tea is a vital perennial beverage variety and is in high demand in global markets due to its quality and medicinal value. In recent years, the cultivation of tea plantations has decreased due to the extreme climate and prolonged practice of tea cultivation in the same area, decreasing its taste and quality. In this scenario, land suitability analysis is the best approach to evaluate the bio-physiochemical and ecological parameters of tea plantations. The present study aims to identify and delineate appropriate land best suited for the cultivation of tea within the Kallar watershed using the geographic information system (GIS) and multi-criteria evaluation (MCE) techniques. This study utilises various suitability criteria, such as soil (texture, hydrogen ion concentration, electrical conductivity, depth, base saturation, and drainability), climate (rainfall and temperature), topography (relief and slope), land use, and the normalised difference vegetation index (NDVI), to evaluate the suitability of the land for growing tea plantations based on the Food and Agricultural Organization (FAO) guidelines for rainfed agriculture. The resultant layers were classified into five suitability classes, including high (S1), moderate (S2), and marginal (S3) classes, which occupied 16.7%, 7.08%, and 16.3% of the land, whereas the currently and permanently not suitable (N1 and N2) classes covered about 18.52% and 29.06% of the total geographic area. This study provides sufficient insights to decision-makers and farmers to support them in making more practical and scientific decisions regarding the cultivation of tea plantations that will result in the increased production of quality tea, and prevent and protect human life from harmful diseases.
Soil Loss Estimation Using Remote Sensing and RUSLE Model in Koromi-Federe Catchment Area of Jos-East LGA, Plateau State, Nigeria
Authors: Ugese Andrew Ayangeaor , Ajiboye Jesugbemi Olaoye , Ibrahim Esther Shupel , Gajere Efron Nduke , Itse Atang , Shaba Halilu Ahmad
Published: November 2022

Soil loss caused by erosion has destroyed landscapes, as well as depositing sterile material on fertile lands and rivers, clogged waterways and accelerated flash floods, declined the populations of fish and other species, and diminish soil fertility. In some places, erosion has also destroyed buildings, caused mudflow, create new landforms, displaced people, and slowed down the economy of the affected community by destroying roads and homes. Erosion is aggravated by climate change and anthropogenic factors such as deforestation, overgrazing, inappropriate methods of tillage, and unsustainable agricultural practices. In this study, remote sensing (RS) and geographic information (GIS) data and tools were used to model erosion and estimate soil loss in the catchment area of Koromi-Federe in Jos East, Plateau State Nigeria which is our study area. Soil loss estimation was performed using the revised universal soil loss equation (RUSLE) model and was computed by substituting the corresponding values of each factor inherent in the equation (rainfall erosivity, soil erodibility, slope steepness and slope length, cover management, and conservation practices) using RS and GIS tools. Soil data was obtained from the study area and analyzed in the laboratory, rainfall data, land cover, digital elevation model (DEM), as well as the management practice of the study area were the parameters computed in spatial analyst tool using map algebra based on RUSLE. The soil loss generated was classified into four classes and the results revealed 95.27% of the catchment with a tolerable loss of less than 10 t/h−1/y−1. At 3.6%, a low or minimal loss of 10–20 t/h−1/y−1, at 1.03% there exists a moderate loss of 20–50 t/h−1/y−1, while there was and critical or high loss of >50 t/h−1/y−1 at 0.12% of the catchment. The result showed that critical soil loss in the catchment area is exacerbated by the influence of the slope length and steepness, and the amount of rainfall received. This poses great concern with annual rainfall projected to increase up to 12% in West Africa. However, our sensitivity analysis revealed that it can be reduced with the effect of vegetated cover and management practices. This is an important finding as it can guide sustainability practices to control erosion and the loss of valuable lands in the region, especially now under climate change.
Driving Cessation: What Are Family Members’ Experiences and What Do They Think about Driving Simulators?
Authors: Knoefel Frank , Mayamuud Salma , Tfaily Rania
Published: November 2022

Background: Driving cessation is difficult for persons living with cognitive decline (PLWCD) and their caregivers (CG). Physicians are often required to notify authorities of driving risks, and typically base decisions on paper-based cognitive assessments and on-road tests. This study examines experiences surrounding cessation and CG’s views regarding simulators in the process. Methods: Semi-structured virtual interviews were conducted with CGs of PLWCD from an academic memory clinic. Experiences around cessation were explored first, followed by discussions regarding the simulator. Framework analysis was applied to transcribed interviews. Results: Six females and two males, three children and five spouses participated. PLWCD viewed driving cessation negatively, often had difficulty understanding why, and believed cessation was temporary. CGs experienced relief and/or shock. Cessation negatively impacted the relationships between the PLWCD and both the physician and CG. Isolation, coping challenges and loss of independence were experienced by the PLWCD. The lives of caregivers were adversely affected, especially regarding driving burden and worsening mental health. CGs were generally supportive of simulators. Positives included: measurement of driving skills, method of testing, and providing an understanding regarding the driving suspension. Potential drawbacks included difficulty using the machine, testing anxiety and stress induced by a crash. Caregivers were concerned about: PLWCD’s disappointment of failure, requesting to retest, and reluctance to accept the decision. Conclusion: PLWCD and caregivers had negative experiences related to the driving cessation. Generally, caregivers viewed implementing driving simulators positively, in a context of a practice session and support for PLWCD’s potential reactions to the decision.
Prevalence of Glenohumeral Internal Rotation Deficit and Sex Differences in Range of Motion of Adolescent Volleyball Players: A Case-Control Study
Authors: Mizoguchi Yasuaki , Suzuki Kenta , Shimada Naoki , Naka Hiroyuki , Kimura Fumihiko , Akasaka Kiyokazu
Published: November 2022

Shoulder range of motion (ROM) adaptation is common observed among volleyball players, but studies on the shoulder joint function of adolescent athletes are lacking. This study aimed to clarify the prevalence of glenohumeral internal rotation deficit (GIRD) among adolescent players and differences in ROM based on sex. A questionnaire survey and ROM measurements of the shoulder joint and trunk using a plastic goniometer were conducted on 123 volleyball players (63 males and 60 females; mean age, 15.8 years). The prevalence of GIRD was investigated for internal rotation differences of >10° and total rotation motion of <5°. Questionnaire items and ROM were compared between GIRD and non-GIRD patients, and sex differences in ROM were also presented. Of the participants, 38.2% (n = 47/123) had GIRD. The GIRD group showed a decrease in external rotation on the dominant side (p = 0.003, 1 − beta = 0.84), but this was not associated with a history of shoulder injury. Sex differences in shoulder ROM showed hypomobility in males and hypermobility in females. However, there was no association between shoulder injury and GIRD among adolescent players. There are sex differences in ROM, which should be considered in future studies.
The Current Concept of Paternal Bonding: A Systematic Scoping Review
Authors: Suzuki Daichi , Ohashi Yukiko , Shinohara Eriko , Usui Yuriko , Yamada Fukiko , Yamaji Noyuri , Sasayama Kiriko , Suzuki Hitomi , Nieva Romulo Fernandez , da Silva Lopes Katharina , Miyazawa Junko , Hase Michiko , Kabashima Minoru , Ota Erika
Published: November 2022

Bonding is crucial to perinatal mental health. Despite an extensive body of literature on maternal bonding, few studies have focused on paternal bonding. This scoping review aimed to clarify the current state of the concept of paternal–infant/fetus bonding. The eligibility criteria were drawn from the population concept and context elements to answer the following questions: “what is paternal bonding?” and “what are the constructs of the concept of paternal bonding?” The review comprised 39 studies. Paternal bonding was associated with both positive and negative paternal behavior and thought and may be determined based on fathers’ beliefs and rearing history. Most studies showed that father–child interaction is one of the factors promoting paternal bonding. However, fathers generally felt more distant from their babies post-delivery than mothers. Only a few studies originally defined paternal bonding; most relied on the definitions of maternal bonding. We found different descriptions lacking consensus. Few studies examined the differences between paternal and maternal bonding. No consensus exists on the concept, constructs, and assessment of paternal bonding. The causal relationship between paternal bonding and other variables is unexplored. Future studies should explore fathers’ perspectives and experiences, focusing on the unknown aspects of paternal bonding identified in this review.
The Impact of COVID-19 on the Monitoring of Pregnancy and Delivery of Pregnant Women in the Dominican Republic
Authors: Requena-Mullor Mar , García-González Jessica , Wei Ruqiong , Romero-del Rey Raúl , Alarcón-Rodríguez Raquel
Published: November 2022

Pregnancy monitoring is vital to guaranteeing that both the foetus and the mother are in optimal health conditions. WHO protocols recommend at least eight medical examinations during the pregnancy period. While the cancellation or reduction of appointments during pregnancy due to the pandemic may help reduce the risk of infection, it could also negatively influence perinatal outcomes and the birthing process. The aim of this research was to analyse the differences in perinatal outcomes and birth characteristics in two groups of pregnant women: women who gave birth before and during the pandemic, and whether these differences are due to changes in pregnancy monitoring because of the COVID-19 situation. A retrospective study was carried out from July 2018 to December 2021, at the Santo Domingo Hospital (Dominican Republic). A total of 1109 primiparous pregnant women were recruited for this study during the birthing process and perinatal visits. The results describe how women who gave birth before the pandemic had greater control and monitoring of their pregnancy, more doctor visits (p = 0.001), fewer caesarean sections (p = 0.006), and more skin-to-skin contact after birth (p = 0.02). During the COVID-19 pandemic, pregnant women’s attendance at routine pregnancy monitoring, both doctor visits and ultrasound scans, has decreased, leading to an increase in the number of caesarean and instrumental deliveries. At the perinatal level, processes such as skin-to-skin contact after birth between mother and newborn or the introduction of early breastfeeding in the delivery room have also been reduced.
Bioelectrical Impedance Analysis Can Be an Effective Tool for Screening Fatty Liver in Patients with Suspected Liver Disease
Authors: Choi Jin Wook , Yoo Jeong-Ju , Kim Sang Gyune , Kim Young Seok
Published: November 2022

Background and study aims: Although abdominal ultrasound (USG) or controlled attenuation parameter (CAP) score of transient elastography (TE) is recommended for the diagnosis of fatty liver, issues regarding cost and accessibility still exist. The aim of this study was to evaluate if bioelectrical impedance analysis (BIA) can be used as a reliable screening tool for fatty liver. Patients and methods: A total of 249 patients who underwent all three tests including TE, BIA, and USG were enrolled. The correlation between fat mass measured by BIA, CAP score of TE, and fatty liver grade measured by USG was analyzed. In addition, the cut-off value of BIA which can predict the fatty liver grade was calculated. Results: Fat mass index (FMI) assessed by BIA increased significantly along with the rise in fatty liver grade measured by USG (normal: 6.2 ± 2.4, Gr I: 8.0 ± 3.7, Gr II: 10.6 ± 3.5, Gr III: 10.7 ± 3.7 kg/m2, p < 0.001). In addition, a positive correlation was found between the CAP score of TE and the FMI of BIA. Additionally, a total body fat mass increase by 24.3% or 29.8% in men and 34.8% or 35.1% in women increased the possibility of developing any grade of fatty liver or significant fatty liver (≥Gr II fatty liver), respectively. Conclusion: The total fat or fat mass index of BIA was related to fatty liver as assessed by ultrasound or CAP score, and area under the receiver operating characteristic (AUROC) was about 0.8. Thus, BIA can be used as a screening tool for fatty liver in patients with suspected liver disease.
The Role of Natural Language Processing during the COVID-19 Pandemic: Health Applications, Opportunities, and Challenges
Authors: Al-Garadi Mohammed Ali , Yang Yuan-Chi , Sarker Abeed
Published: November 2022

The COVID-19 pandemic is the most devastating public health crisis in at least a century and has affected the lives of billions of people worldwide in unprecedented ways. Compared to pandemics of this scale in the past, societies are now equipped with advanced technologies that can mitigate the impacts of pandemics if utilized appropriately. However, opportunities are currently not fully utilized, particularly at the intersection of data science and health. Health-related big data and technological advances have the potential to significantly aid the fight against such pandemics, including the current pandemic’s ongoing and long-term impacts. Specifically, the field of natural language processing (NLP) has enormous potential at a time when vast amounts of text-based data are continuously generated from a multitude of sources, such as health/hospital systems, published medical literature, and social media. Effectively mitigating the impacts of the pandemic requires tackling challenges associated with the application and deployment of NLP systems. In this paper, we review the applications of NLP to address diverse aspects of the COVID-19 pandemic. We outline key NLP-related advances on a chosen set of topics reported in the literature and discuss the opportunities and challenges associated with applying NLP during the current pandemic and future ones. These opportunities and challenges can guide future research aimed at improving the current health and social response systems and pandemic preparedness.
Health Information System and Health Care Applications Performance in the Healthcare Arena: A Bibliometric Analysis
Authors: Epizitone Ayogeboh , Moyane Smangele Pretty , Agbehadji Israel Edem
Published: November 2022

There have been several studies centred on health information systems with many insights provided to enhance health care applications globally. These studies have provided theoretical schemes for fortifying the enactment and utilisation of the Health Information System (HIS). In addition, these research studies contribute greatly to the development of HIS in alignment with major stakeholders such as health practitioners and recipients of health care. Conversely, there has been trepidation about HIS’ sustainability and resilience for healthcare applications in the era of digitalization and globalization. Hence, this paper investigates research on HIS with a primary focus on health care applications to ascertain its sustainability and resilience amidst the transformation of the global healthcare space. Therefore, using a bibliometric approach, this paper measures the performance of health information systems and healthcare for health care applications using bibliometric data from the web of science database. The findings reveal solid evidence of the constructive transformation of health information systems and health care applications in the healthcare arena, providing ample evidence of the adaptation of HIS and health care applications within the healthcare arena to the fourth industrial revolution and, additionally, revealing the resilient alignment of health care applications and health information systems.
Smoking Addiction in Patients with Schizophrenia Spectrum Disorders and Its Perception and Intervention in Healthcare Personnel Assigned to Psycho-Rehabilitation Programs: A Qualitative Research
Authors: Caponnetto Pasquale , Maglia Marilena , Mangione Marta , Vergopia Chiara , Prezzavento Graziella Chiara , Polosa Riccardo , Quattropani Maria Catena , DiPiazza Jennifer , Signorelli Maria Salvina
Published: November 2022

Patients with schizophrenia spectrum disorders have a higher prevalence and frequency of smoking rates when compared to the rest of the population; to this, it must be added that they develop a greater dependence and have some worse health consequences than the general population. This is qualitative research on the perception of smoking in healthcare professionals assigned to psycho-rehabilitation programs for patients with schizophrenia spectrum disorders. The point of view of health personnel (Psychologists, Psychiatrists, Pedagogists, and Nurses) about cigarette smoking in these patients was analyzed, focusing on their implications in disturbance and comparing them with e-cigarettes too. The methodology used to collect the data was a semi-structured interview with five questions. The research path was carried out in two assisted therapeutic communities that are clinics for the rehabilitation of serious mental illness in the period between November and July 2022. The results showed that the opinion of health professionals on smoking is very negative. Research has also shown that nearly all patients are smokers; however, their high grade of addiction is caused by periods of high stress due to various factors that lead patients to consume a greater number of cigarettes. Almost all respondents have a positive opinion of the e-cigarette, which was defined as an excellent substitute for traditional cigarettes.
Deriving Meaning from Chaos: The Mediating Role of the Sense of Coherence in the Serial Relationships among Fear of COVID-19, Indices of Psychological Distress, and Life Satisfaction
Authors: Pretorius Tyrone B. , Padmanabhanunni Anita
Published: November 2022

The current study focused on the role of the sense of coherence (SOC) in the serial relationships among the fear of COVID-19, indices of psychological distress, and life satisfaction. It examined the hypothesis that an SOC would mitigate the impact of the fear of COVID-19 on psychological distress, which in turn would positively impact life satisfaction. Participants were school teachers (N = 355) who completed the Fear of COVID-19 Scale, the trait scale of the State-Trait Anxiety Inventory, the Beck Hopelessness Scale, the short form of the Sense of Coherence Scale, and the Satisfaction with Life Scale. A path analysis confirmed the mediating role of the dimensions of the SOC in the relationships among the fear of COVID-19, indices of psychological distress, and life satisfaction. Specifically, comprehensibility, meaningfulness, and manageability were found to mediate the associations between the fear of COVID-19 and anxiety, and the fear of COVID-19 and hopelessness, which in turn were associated with higher levels of life satisfaction. The findings confirmed that an SOC is an important source of resilience. Interventions that facilitate the re-appraisal of stressors as challenges and enhance the awareness of practical coping strategies can build an SOC and promote mental health.
Effect of Photo-Selective Nets on Yield, Fruit Quality and Psa Disease Progression in a ‘Hayward’ Kiwifruit Orchard
Authors: Moura Luísa , Pinto Rui , Rodrigues Raul , Brito Luís Miguel , Rego Rute , Valín Maria Isabel , Mariz-Ponte Nuno , Santos Conceição , Mourão Isabel Maria
Published: November 2022

The influence of the colour of photo-selective nets on Actinidia deliciosa yield, fruit quality and progression of the bacterial kiwifruit canker (Pseudomonas syringae pv. actinidiae, Psa) need to be characterised due to increasing use of these nets, mainly to protect from hail and storms. From May 2019 onwards, pearl (Pn), yellow (Yn) and grey (Gn) nets were installed permanently in a ‘Hayward’ kiwifruit orchard in NW Portugal and uncovered plants were used as the control. Compared to outside conditions for both seasons, the blue:red ratio and the mean air temperature were higher (mean increase of 12.7% and 0.6 °C, respectively) and the photosynthetic active radiation (PAR) was lower (10.8% less between budbreak and bloom) under the Pn. Crop yield, compared to the control, decreased by 40.3% under the three nets in 2020, and by 23.9% under the Yn and Gn in 2021. Yield and fruit grade under the Pn were similar to that of uncovered crops in 2021, and fruit grade was overall higher under the Pn compared to the Yn and Gn. Photo-selective nets did not affect the fruit quality parameters. Psa progression decreased under the Pn compared to the control during two months in both seasons, although this beneficial impact needs further evaluation.
Estimation of Seed Longevity in Onion Seed Lots by a Vigor Test of Radicle Emergence Test in Artificial Ageing Conditions
Authors: Demir Ibrahim , Kuzucu Canan Oztokat , Ermis Sıtkı , Memis Nurcan , Kadioglu Neslihan
Published: November 2022

The study was conducted to test whether a vigor test of a single count of radicle emergence (RE test) would correlate with the storage potential of 10 seed lots of onion (Allium cepa L.) cultivars in artificial storage conditions. The RE test was performed by counting radicle emergence (2 mm) percentage at 80 h after germination was set up at 20 °C. Seed longevity was determined by storage at 75% relative humidity, obtained by saturated NaCl, at 35 °C over 60 days. Twelve seed samples were taken out during ageing, and seed survival curves were constructed based on normal germination percentages conducted at 20 °C for 12 days in the dark. The seed longevity criterion was P50 (time for the viability to fall to 50%), which was determined through probit analysis by using survival curves. Correlation analysis showed that RE counts at 80 h during germination were highly correlated (p < 0.001) with initial seed quality, Ki (R2 = 0.888) and the half-viability period, P50 (R2 = 0.823). Non-aged pre-storage final normal germination percentages of seed lots were also significantly related to longevity (Ki, R2 = 0.808, p < 0.001, P50, R2 = 0.750, p <0.01), while the level of significance was lower. It can be concluded that the RE test can be used as a fast vigor test to estimate the longevity in onion seed lots.
The Role of Arrestin-1 Middle Loop in Rhodopsin Binding
Authors: Vishnivetskiy Sergey A. , Huh Elizabeth K. , Karnam Preethi C. , Oviedo Samantha , Gurevich Eugenia V. , Gurevich Vsevolod V.
Published: November 2022

Arrestins preferentially bind active phosphorylated G protein-coupled receptors (GPCRs). The middle loop, highly conserved in all arrestin subtypes, is localized in the central crest on the GPCR-binding side. Upon receptor binding, it directly interacts with bound GPCR and demonstrates the largest movement of any arrestin element in the structures of the complexes. Comprehensive mutagenesis of the middle loop of rhodopsin-specific arrestin-1 suggests that it primarily serves as a suppressor of binding to non-preferred forms of the receptor. Several mutations in the middle loop increase the binding to unphosphorylated light-activated rhodopsin severalfold, which makes them candidates for improving enhanced phosphorylation-independent arrestins. The data also suggest that enhanced forms of arrestin do not bind GPCRs exactly like the wild-type protein. Thus, the structures of the arrestin-receptor complexes, in all of which different enhanced arrestin mutants and reengineered receptors were used, must be interpreted with caution.
Overexpression of a Plasma Membrane H+-ATPase Gene OSA1 Stimulates the Uptake of Primary Macronutrients in Rice Roots
Authors: Ding Ming , Zhang Maoxing , Wang Zihui , Yu Xin , Kinoshita Toshinori , Zeng Houqing , Zhu Yiyong
Published: November 2022

Plasma membrane (PM) H+-ATPase is a master enzyme involved in various plant physiological processes, such as stomatal movements in leaves and nutrient uptake and transport in roots. Overexpression of Oryza sativa PM H+-ATPase 1 (OSA1) has been known to increase NH4+ uptake in rice roots. Although electrophysiological and pharmacological experiments have shown that the transport of many substances is dependent on the proton motive force provided by PM H+-ATPase, the exact role of PM H+-ATPase on the uptake of nutrients in plant roots, especially for the primary macronutrients N, P, and K, is still largely unknown. Here, we used OSA1 overexpression lines (OSA1-oxs) and gene-knockout osa1 mutants to investigate the effect of modulation of PM H+-ATPase on the absorption of N, P, and K nutrients through the use of a nutrient-exhaustive method and noninvasive microtest technology (NMT) in rice roots. Our results showed that under different concentrations of P and K, the uptake rates of P and K were enhanced in OSA1-oxs; by contrast, the uptake rates of P and K were significantly reduced in roots of osa1 mutants when compared with wild-type. In addition, the net influx rates of NH4+ and K+, as well as the efflux rate of H+, were enhanced in OSA1-oxs and suppressed in osa1 mutants under low concentration conditions. In summary, this study indicated that overexpression of OSA1 stimulated the uptake rate of N, P, and K and promoted flux rates of cations (i.e., H+, NH4+, and K+) in rice roots. These results may provide a novel insight into improving the coordinated utilization of macronutrients in crop plants.
Transcriptome Analysis of Two Tetraploid Potato Varieties under Water-Stress Conditions
Authors: Alvarez-Morezuelas Alba , Barandalla Leire , Ritter Enrique , Ruiz de Galarreta Jose Ignacio
Published: November 2022

Potato (Solanum tuberosum L.) is one of the most important crops worldwide, but due to its sensitivity to drought, its production can be affected by water availability. In this study, the varieties Agria and Zorba were used to determine the expression differences between control and water-stressed plants. For this purpose, they were sequenced by RNAseq, obtaining around 50 million transcripts for each variety and treatment. When comparing the significant transcripts obtained from control and drought-stressed plants of the Agria variety, we detected 931 genes that were upregulated and 2077 genes that were downregulated under stress conditions. When both treatments were compared in Zorba plants, 735 genes were found to be upregulated and 923 genes were found to be downregulated. Significantly more DEGs were found in the Agria variety, indicating a good stress response of this variety. “Abscisic acid and environmental stress-inducible protein TAS14-like” was the most overexpressed gene under drought conditions in both varieties, but expression differences were also found in numerous transcription factors and heat shock proteins. The principal GO term found was “cellular components”, more specifically related to the cell membrane and the cell wall, but other metabolic pathways such as carbohydrate metabolism and osmotic adjustment were also identified. These results provide valuable information related to the molecular mechanisms of tolerance to water stress in order to establish the basis for breeding new, more tolerant varieties.