bluuebunny/crossref_metadata_2025 huggingface dataset:
Bond Durability of Two-Step HEMA-Free Universal Adhesive
Authors: Tsujimoto Akimasa , Fischer Nicholas G. , Barkmeier Wayne W. , Latta Mark A.
Published: August 2022

The purpose of this study is to compare bond durability, in terms of fatigue bond strength, of a two-step HEMA-free universal adhesive and representative adhesives in each systematic category. The adhesives used in this study were OptiBond FL, Prime&Bond NT, Clearfil SE Bond 2, G2-Bond Universal, and Scotchbond Universal Plus Adhesive. Fatigue bond strength testing and scanning electron microscopy analysis of adhesively bonded enamel and dentin interfaces were performed. For the adhesives in etch-and-rinse mode, the enamel fatigue bond strength of the G2-Bond Universal adhesive was significantly higher than those of other adhesives, and the dentin fatigue bond strength of Prime&Bond NT was significantly lower than the others. For adhesives in self-etch mode, the enamel fatigue bond strengths of Clearfil SE Bond 2 and G2-Bond Universal were significantly higher than that of the Scotchbond Universal Plus Adhesive, and the dentin fatigue bond strength of G2-Bond Universal was significantly higher than Clearfil SE Bond 2 and the Scotchbond Universal Plus Adhesive. The two-step HEMA-free universal adhesive showed higher enamel and higher or equal dentin fatigue bond strength than other selected representative adhesive systems in etch-and-rinse mode and higher or equal enamel and higher dentin fatigue bond strength than adhesive systems in self-etch mode.
RETRACTED: Amplitude and Phase Computable Ocean Wave Real-Time Modeling with GPU Acceleration
Authors: Wang Guigui , Tan Shihan , Song Ge , Wang Sheng
Published: August 2022

The CBATS (carrier-based aircraft take-off and landing training system) is an important application of virtual reality technology in the simulation field. Large-scale, real-time ocean simulations are the biggest challenge to the authenticity of the visual system of CBATS and are also currently the main research hotspot in the field of computer graphics. In this paper, a hybrid Ocean Modeling Method based on wavelet transform is presented. This method introduces an accurate phase calculation and a wind-field model solution to compensate for the randomness of wave generation and the lack of physical mechanism in spectral methods. The computational cost is greatly reduced by using a rough spatial grid to calculate the amplitude and phase values at any point in space, which effectively avoids Nyquist–Shannon Theorem limitations caused by the numerical solutions of PDEs (partial differential equations), and a high-fidelity simulation of high frequency, detailed sea surface and coherent phase-dependent wave effects is achieved. Practical verification shows that the method can fully meet the real-time simulation training requirements of CBATS with a strong real-time performance and good stability. Thus, it could play a significant role in improving the performance of the visual system.
De Novo Purine Nucleotide Biosynthesis Pathway Is Required for Development and Pathogenicity in Magnaporthe oryzae
Authors: Liu Meng-Yu , Sun Li-Xiao , Qian Hui , Zhang Yun-Ran , Zhu Xue-Ming , Li Lin , Liang Shuang , Lu Jian-Ping , Lin Fu-Cheng , Liu Xiao-Hong
Published: August 2022

Purine nucleotides are indispensable compounds for many organisms and participate in basic vital activities such as heredity, development, and growth. Blocking of purine nucleotide biosynthesis may inhibit proliferation and development and is commonly used in cancer therapy. However, the function of the purine nucleotide biosynthesis pathway in the pathogenic fungus Magnaporthe oryzae is not clear. In this study, we focused on the de novo purine biosynthesis (DNPB) pathway and characterized MoAde8, a phosphoribosylglycinamide formyltransferase, catalyzing the third step of the DNPB pathway in M. oryzae. MoAde8 was knocked out, and the mutant (∆Moade8) exhibited purine auxotroph, defects in aerial hyphal growth, conidiation, and pathogenicity, and was more sensitive to hyperosmotic stress and oxidative stress. Moreover, ∆Moade8 caused decreased activity of MoTor kinase due to blocked purine nucleotide synthesis. The autophagy level was also impaired in ∆Moade8. Additionally, MoAde5, 7, 6, and 12, which are involved in de novo purine nucleotide biosynthesis, were also analyzed, and the mutants showed defects similar to the defects of ∆Moade8. In summary, de novo purine nucleotide biosynthesis is essential for conidiation, development, and pathogenicity in M. oryzae.
The Influence of Urbanization to the Outer Boundary Ecological Environment Using Remote Sensing and GIS Techniques—A Case of the Greater Bay Area
Authors: Zhang Qingyang , Cai Xinyan , Liu Xiaoliang , Yang Xiaomei , Wang Zhihua
Published: August 2022

Urbanization brings great enrichment to human production and life, but also has certain environmental impact on the area where the city is located. Many studies have revealed the negative effects of urbanization on the ecological environment of urban or urban agglomerations, especially in the early stage of urbanization, but there are few studies on the impact on the peripheral ecological space environment. Will the peripheral environment be better off with less human interference as people move to cities during urbanization? In order to answer this question, we took the Guangdong-Hong Kong-Macao Greater Bay Area, the most economically dynamic area in China, as an example to explore the relationship between impervious changes of urban agglomerations monitored by remote sensing in the Bay Area and ecological indicators of forest and grassland in Guangdong Province outside the Bay area. The results showed that:(1) in the past 30 years, the area of grassland outside the bay area did not change regularly, while the area of forest decreased year by year. The landscape indices of forest and grassland were gradually fragmented and discrete. Moreover, the distribution of Fraction Vegetation Coverage (FVC) of forest and grassland has changed since before urbanization. (2) Through correlation analysis, it is found that the changes in forest area and the landscape index of forest and grassland are strongly correlated with the development of urbanization in the Greater Bay Area. This shows that the process of urbanization in the Greater Bay Area will have a non-negligible impact on the peripheral environment. In the process of urban development, we should not only focus on the inner city but also consider the outer environment of the city.
Laboratory Experiments on the In Situ Upgrading of Heavy Crude Oil Using Catalytic Aquathermolysis by Acidic Ionic Liquid
Authors: D. Alharthy Rima , El-Nagar Raghda A. , Ghanem Alaa
Published: August 2022

Heavy and extra heavy oil exploitation has attracted attention in the last few years because of the decline in the production of conventional crude oil. The high viscosity of heavy crude oil is the main challenge that obstructs its extraction. Consequently, catalytic aquathermolysis may be an effective solution to upgrade heavy crude oil to decrease its viscosity in reservoir conditions. In this regard, a series of acidic ionic liquids, 1-butyl-1H-imidazol-3-ium 4-dodecylbenzenesulfonate (IL-4), 1-decyl-1H-imidazol-3-ium 4-dodecylbenzenesulfonate (IL-10), and 1-hexadecyl-1H-imidazol-3-ium 4-dodecylbenzenesulfonate (IL-16), were utilized in the aquathermolysis of heavy crude oil. Of each IL, 0.09 wt % reduced the viscosity of the crude oil by 89%, 93.7%, and 94.3%, respectively, after the addition of 30% water at 175 °C. ILs with alkyl chains equal to 10 carbon atoms or more displayed greater activity in viscosity reduction than that of ILs with alkyl chains lower than 10 carbon atoms. The molecular weight and asphaltene content of the crude oil were decreased after catalytic aquathermolysis. The compositional analysis of the crude oil before and after catalytic aquathermolysis showed that the molar percentage of lighter molecules from tridecanes to isosanes was increased by 26–45%, while heavier molecules such as heptatriacontanes, octatriacontanes, nonatriacontanes, and tetracontanes disappeared. The rheological behavior of the crude oil before and after the catalytic aquathermolytic process was studied, and the viscosity of the crude oil sample was reduced strongly from 678, 29.7, and 23.4 cp to 71.8, 16.9, and 2.7 cp at 25, 50, and 75 °C, respectively. The used ILs upgraded the heavy crude oil at a relatively low temperature.
A Biocompatibility Study of Plasma Nanocoatings onto Cobalt Chromium L605 Alloy for Cardiovascular Stent Applications
Authors: Phan Thithuha , Jones John E. , Chen Meng , Bowles Doug K. , Fay William P. , Yu Qingsong
Published: August 2022

The objective of this study was to evaluate the biocompatibility of trimethylsilane (TMS) plasma nanocoatings modified with NH3/O2 (2:1 molar ratio) plasma post-treatment onto cobalt chromium (CoCr) L605 alloy coupons and stents for cardiovascular stent applications. Biocompatibility of plasma nanocoatings was evaluated by coating adhesion, corrosion behavior, ion releasing, cytotoxicity, and cell proliferation. Surface chemistry and wettability were studied to understand effects of surface properties on biocompatibility. Results show that NH3/O2 post-treated TMS plasma nanocoatings are hydrophilic with water contact angle of 48.5° and have a typical surface composition of O (39.39 at.%), Si (31.92 at.%), C (24.12 at.%), and N (2.77 at.%). The plasma nanocoatings were conformal to substrate surface topography and had excellent adhesion to the alloy substrates, as assessed by tape test (ASTM D3359), and showed no cracking or peeling off L605 stent surfaces after dilation. The plasma nanocoatings also improve the corrosion resistance of CoCr L605 alloy by increasing corrosion potential and decreasing corrosion rates with no pitting corrosion and no mineral adsorption layer. Ion releasing test revealed that Co, Cr, and Ni ion concentrations were reduced by 64–79%, 67–69%, and 57–72%, respectively, in the plasma-nanocoated L605 samples as compared to uncoated L605 control samples. The plasma nanocoatings showed no sign of cytotoxicity from the test results according to ISO 10993-05 and 10993-12. Seven-day cell culture demonstrated that, in comparison with the uncoated L605 control surfaces, the plasma nanocoating surfaces showed 62 ± 7.3% decrease in porcine coronary artery smooth muscle cells (PCASMCs) density and had comparable density of porcine coronary artery endothelial cells (PCAECs). These results suggest that TMS plasma nanocoatings with NH3/O2 plasma post-treatment possess the desired biocompatibility for stent applications and support the hypothesis that nanocoated stents could be very effective for in-stent restenosis prevention.
Valorization of Polypropylene Waste in the Production of New Materials with Adequate Mechanical and Thermal Properties for Environmental Protection
Authors: Râpă Maria , Spurcaciu Bogdan Norocel , Ion Rodica-Mariana , Grigorescu Ramona Marina , Darie-Niță Raluca Nicoleta , Iancu Lorena , Nicolae Cristian-Andi , Gabor Augusta Raluca , Matei Ecaterina , Predescu Cristian
Published: August 2022

Innovative composites based on polypropylene waste impurified cu HDPE (PPW) combined with two thermoplastic block-copolymers, namely styrene-butadiene-styrene (SBSBC) and styrene-isoprene-styrene (SISBC) block-copolymers, and up to 10 wt% nano-clay, were obtained by melt blending. SBSBC and SISBC with almost the same content of polystyrene (30 wt%) were synthesized by anionic sequential polymerization and used as compatibilizers for PPW. Optical microscopy evaluation of the PPW composites showed that the n-clay was encapsulated into the elastomer. Addition of n-clay, together with SBSBC or SISBC, increased the interphase surface of the components in the PPW composites and enhanced the superficial area/volume ratio, which led to a recycled material with improved performance. The data resulting from differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), mechanical evaluation, and dynamic mechanical analysis (DMA) revealed that PPW reinforcement with n-clay and styrene-diene block-copolymers allows the obtaining of composites with favorable mechanical and thermal properties, and excellent impact strength for potential engineering applications.
Fly Ash from Lignite Combustion as a Filler for Rubber Mixes—Part II: Chemical Valorisation of Fly Ash
Authors: Orczykowski Wojciech , Bieliński Dariusz M. , Anyszka Rafał , Gozdek Tomasz , Klajn Katarzyna , Celichowski Grzegorz , Pędzich Zbigniew , Wojteczko Agnieszka
Published: August 2022

Fly ash (FA) fractions with a particle size of 63 µm < FA < 250 µm obtained by sieve fractionation were used as a partial carbon black (CB) replacement in a rubber mixture based on styrene-butadiene rubber (SBR). In order to improve the interactions at the interface between rubber and fractionated ash, at the stage of preparing the rubber mixtures, two different vinyl silanes were added to the system: Vinyltrimethoxysilane (U-611) or Vinyl-tris (2-methoxy-ethoxy) silane (LUVOMAXX VTMOEO DL50), silane with epoxy groups: 3-(glycidoxypropyl)trimethoxysilane (U-50) or sulfur functionalized silanes: containing sulfide bridges: Bis(triethoxysilylpropyl)polysulfide silane (Si-266) or mercapto groups: Mercaptopropyltrimethoxysilane (Dynaslan MTMO). The conducted research confirmed the effectiveness of silanization with selected functional silanes, from the point of view of improving the processing and operational properties of vulcanizates, in which CB is partially replaced with the finest fractions of fly ash. The silanization generally increased the interaction at the rubber–ash interface, while improving the degree of filler dispersion in the rubber mixture. The results of TGA and FTIR analyses confirmed the presence of silanes chemically bonded to the surface of fly ash particles. SEM tests and determination of the bound rubber (BdR) content show that the introduction of the silanes to the mixture increases the degree of ash dispersion (DI) and the Payne effect, which is the greatest when mercaptosilane was used for modification. The highest increase in torque, which was recorded in the case of rubber mixtures containing sulfur silanes and silane with epoxy groups, may be due to their participation in the vulcanization process, which is confirmed by the results of vulcametric studies. The lowest values of mechanical strength, elongation at break, and the highest hardness of vulcanizates obtained in this case may be the result of the over-crosslinking of the rubber. The addition of sulfur-containing silanes significantly slowed down the vulcanization process, which is particularly visible (up to three times extension of the t90 parameter, compared to mixtures without silane) in the case of Si-266. The addition of silanes, except for Si-266 (with a polysulfide fragment), generally improved the abrasion resistance of vulcanizates. The Dynaslan MTMO silane (with mercapto groups) performs best in this respect. Proper selection of silane for the finest fraction of fly ash in the rubber mixtures tested allows for an increase in the mechanical strength of their vulcanizates from 9.1 to 17 MPa, elongation at break from 290 to 500%, hardness from 68 to 74 °ShA, and reduction in abrasion from 171 to 147 mm3.
The Transition of Mg-Containing Phases and Recovery of NaCl in Molten Salt Chloride Slags at High Temperature
Authors: Chen Feng , Wen Yuekai , Guo Yufeng , Wang Shuai , Yang Lingzhi , Zheng Yu , Li Dongyue , Ren Yuqiao
Published: August 2022

The molten salt chlorination method is more suitable to produce TiCl4 using titanium-containing materials with high contents of CaO and MgO in China. However, there is a large amount of molten salt chloride slags generated from the molten salt chlorination process, which contains a variety of chlorides and is difficult to treat, often causing serious environmental problems such as direct piling or landfilling. A novel process was proposed to deal with molten salt chloride slags, and calcium chloride could be effectively removed by this process (as in our previous study). However, magnesium chloride is another impurity which can deteriorate the density and viscosity of the molten salt; it is often found in higher contents, and should be also removed from molten salt chloride slags to efficiently recycle NaCl in novel processes. Na2SiO3 is added to the molten salt chloride slags in the molten state to produce the Mg-containing solid phase, which could be separated with the molten NaCl in novel processes. Thus, the transition of Mg-containing phases and the recovery of NaCl in molten salt chloride slags at high temperature in a novel process were systematically investigated in this work, including thermodynamic analysis, the phase transition behavior of Mg-containing phases, NaCl recovery, etc. The removal rate of Mg was 99.56% when the molar ratio of MgCl2:Na2SiO3 was 1:1.5 at 1273 K and in a N2 atmosphere. The recovery rate of NaCl from the molten salt chlorination slag was 97.62% and the purity of NaCl obtained was 99.35 wt%, which could be used in the molten salt chlorination process.
Correlation Study between the Mechanical Property of the Square Cone Energy-Absorbing Structure and Collision Energy Distribution for Urban Rail Trains
Authors: Xu Ping , Wang Ao , Yang Liting , Che Quanwei , Yang Chengxing
Published: August 2022

In order to study the influence of the square cone energy-absorbing structure of urban rail trains on the collision energy distribution of trains during the collision process, an eight-marshalling train crash dynamics model was established, and the force–displacement energy-absorbing characteristic curve of the anti-climbing structure was obtained through a full-scale impact experiment. According to the characteristics of the curve, the influence of the initial peak force, the platform force and the slope of the platform force of the anti-climbing energy-absorbing device on the energy absorption of the train collision under a 25 km/h condition was studied. Based on this, the multi-objective genetic algorithm was used to optimize the overall energy distribution scheme of the train. The results showed that the changes in the initial peak force, the platform force and the slope of the platform force will lead to changes in the energy absorption of the four-section moving train and the four-section stationary train that are closer to the collision interface, and the other is almost unchanged. Through the optimized design, the energy absorption of the head car impact interface is increased by 0.74%, the energy absorption of the first impact interface of the middle cars is reduced by 3.36% and the third impact interface of the middle cars is increased by 9.17%. The distribution of the collision energy of the train is more reasonable. At the same time, the deformation of the middle car body is reduced, and the utilization efficiency of the overall energy-absorbing structure of the train is improved.
Variable Neighborhood Search Algorithms to Solve the Electric Vehicle Routing Problem with Simultaneous Pickup and Delivery
Authors: Yilmaz Yusuf , Kalayci Can B.
Published: August 2022

This paper addresses the Electric Vehicle Routing Problem with Simultaneous Pickup and Delivery (EVRP-SPD), in which electric vehicles (EVs) simultaneously deliver goods to and pick up goods from customers. Due to the limited battery capacity of EVs, their range is shorter than that of internal combustion vehicles. In the EVRP, in addition to the depot and the customers, there are also charging stations (CS) because EVs need to be charged when their battery is empty. The problem is formulated as an integer linear model, and an efficient solution is proposed to minimize the total distance traveled. To create a feasible initial solution, Clarke and Wright’s savings algorithm is used. Several variants of variable neighborhood search are tested, and the reduced-variable neighborhood search algorithm is used to find the best solution in a reasonable time. Computer experiments are performed with benchmark instances to evaluate the effectiveness of our approach in terms of solution quality and time. The obtained results show that the proposed method can achieve efficient solutions in terms of solution quality and time in all benchmark instances.
A Cross-Sectional Study Examining the Association between Physical Activity and Perinatal Depression
Authors: Soto-Fernández Irene , Gómez-Cantarino Sagrario , Yáñez-Araque Benito , Sánchez-Infante Jorge , Zapata-Ossa Alejandra , Dios-Aguado Mercedes
Published: August 2022

Background and Objectives: International organisations recommend that women without illness should have regular moderate-intensity physical exercise throughout their pregnancy and postpartum period as a measure to prevent possible pathologies in both the mother and the newborn. Physical activity during pregnancy reduces the likelihood of depression during pregnancy and after childbirth, benefiting both the pregnant woman and the foetus. However, most pregnant women are known to be inactive. The Pregnancy Physical Activity Questionnaire (PPAQ) analyses the level of physical activity of pregnant women. These data are correlated with the variable depression, for which the Edinburgh Postnatal Depression Scale (EPDS) during pregnancy was used. Materials and Methods: The research employed a cross sectional study design on ninety-nine pregnant women. Results: The data on physical activity in relation to depression in those pregnant women who had not previously suffered from depression were 719.29 METS min/wk compared with 624.62 METS min/wk in those who had. And for pregnant women who suffered from depression at the time of the study, their physical activity was 698.25 METS min/wk, while those who did not suffer from depression reached 826.57 METS. Conclusions: Pregnant women without depression are much more active. A favourable employment situation or a high level of education is directly related to higher physical activity. Physical activity and higher energy expenditure occur at home, as opposed to activity carried out as transport, exercise or at work.
Study on Mechanical Properties and Deformation Mechanism of TWIP Stainless Steel
Authors: Zeng Zeyao , Zhao Jiqing , Luo Xu , Yang Gang , Yin Huifang
Published: August 2022

In this study, based on the sensitivity of the chemical composition fluctuation to the thermodynamic parameter, which controls the level of the stacking fault energy (SFE), a series of high Cr–Mn–N twinning-induced plasticity (TWIP) stainless steels are designed by using a sublattice model, and their mechanical properties and micro deformation mechanism are analyzed The formation of mechanical twins (Mts) during the deformation makes the test steel show a perfect combination of strength and ductility after different solution treatments. Among them, after a solution treatment at 950 °C and 1050 °C, the 19Cr–0.7N and 19CrSi–0.7N samples have the maximum value with the product of the strength and plasticity reaching 60.7% and 64.6%, and 12Cr–CN has the maximum value after the solution treatment at 1200 °C, reaching 81.3%. The SFE values of the 19Cr–0.7N and 19CrSi–0.7N samples were relatively high, 48 mJ·m−2 and 45 mJ·m−2, respectively. The SFE of 12Cr–CN is 37 mJ·m−2, and the Mts grow rapidly during the deformation and maintain the highest twinning density under the same strain conditions. The characterization of the tensile samples occurs under different deformations by electron backscattered diffraction (EBSD) and transmission electron microscope (TEM). The results of the EBSD local misorientation difference angle analysis showed the Silicon element addition with a good Mts saturation rate. It is observed from the TEM that the nucleation process of the Mts with a high SFE is difficult, and the Mts emit and grow inward along the grain boundary during the tensile process and present a cross shape with the increase in strain. The contribution of the grain boundary strengthening (σ0), dislocation strengthening (σf), and twinning strengthening effect (σt) under dynamic micro-refinement to stress were calculated. It is known that under a certain amount of strain, the ratio of σt and σf changes with increasing, and when the contribution of the twinning deformation to the stress exceeds about 25%, the reinforcement of the plastic deformation is dominated by the plane of σf.
Investigation on Thermally Radiative Mixed Convective Flow of Carbon Nanotubes/Al2O3 Nanofluid in Water Past a Stretching Plate with Joule Heating and Viscous Dissipation
Authors: Prabakaran R. , Eswaramoorthi S. , Loganathan Karuppusamy , Sarris Ioannis E.
Published: August 2022

The nature of this prevailing inquisition is to scrutinize the repercussion of MHD mixed convective flow of CNTs/Al2O3 nanofluid in water past a heated stretchy plate with injection/suction, heat consumption and radiation. The Joule heating and viscous dissipation are included in our investigation. The Navier–Stokes equations are implemented to frame the governing flow expressions. These flow expressions are non-dimensioned by employing suitable transformations. The converted flow expressions are computed numerically by applying the MATLAB bvp4c procedure and analytically by the HAM scheme. The impacts of relevant flow factors on fluid velocity, fluid temperature, skin friction coefficient, and local Nusselt number are illustrated via graphs, tables and charts. It is unequivocally shown that the fluid speed declines when escalating the size of the magnetic field parameter; however, it is enhanced by strengthening the Richardson number. The fluid warmness shows a rising pattern when enriching the Biot number and heat consumption/generation parameter. The findings conclusively demonstrate that the surface drag force improves for a larger scale of Richardson number and is suppressed when heightening the unsteady parameter. In addition, it is evident from the outcomes that the heat transfer gradient decreases to increase the quantity of the Eckert number in the convective heating case; however, the opposite nature is obtained in the convective cooling case. Our numerical results are novel, unique and applied in microfluid devices such as micro-instruments, sleeve electrodes, nerve growth electrodes, etc.
Fabrication of Gold Nanostructures on Quartz Crystal Microbalance Surface Using Nanoimprint Lithography for Sensing Applications
Authors: Nishitsuji Ryosuke , Sueyoshi Kenji , Hisamoto Hideaki , Endo Tatsuro
Published: August 2022

A quartz crystal microbalance (QCM) is a sensor that uses the piezoelectric properties of quartz crystals sandwiched between conductive electrodes. Localized surface plasmon resonance (LSPR) is an analytical technique that uses the collective vibration of free electrons on metal surfaces. These measurements are known as analysis techniques that use metal surfaces and have been applied as biosensors because they allow for the label-free monitoring of biomolecular binding reactions. These measurements can be used in combination to analyze the reactions that occur on metal surfaces because different types of information can be obtained from them. However, as different devices are used for these measurements, the results often contain device-to-device errors and are not accurately evaluated. In this study, we directly fabricated gold nanostructures on the surface of a QCM to create a device that can simultaneously measure the mass and refractive index information of the analyte. In addition, the device could be easily fabricated because nanoimprint lithography was used to fabricate gold nanostructures. As a proof of concept, the nanoparticle adsorption on gold nanostructures was evaluated, and it was observed that mass and refractive index information were successfully obtained without device-to-device errors.
Screening and Characterization of New Acetobacter fabarum and Acetobacter pasteurianus Strains with High Ethanol–Thermo Tolerance and the Optimization of Acetic Acid Production
Authors: El-Askri Taoufik , Yatim Meriem , Sehli Youness , Rahou Abdelilah , Belhaj Abdelhaq , Castro Remedios , Durán-Guerrero Enrique , Hafidi Majida , Zouhair Rachid
Published: August 2022

The production of vinegar on an industrial scale from different raw materials is subject to constraints, notably the low tolerance of acetic acid bacteria (AAB) to high temperatures and high ethanol concentrations. In this study, we used 25 samples of different fruits from seven Moroccan biotopes with arid and semi-arid environmental conditions as a basic substrate to isolate thermo- and ethanol-tolerant AAB strains. The isolation and morphological, biochemical and metabolic characterization of these bacteria allowed us to isolate a total number of 400 strains with characters similar to AAB, of which six strains (FAGD1, FAGD10, FAGD18 and GCM2, GCM4, GCM15) were found to be mobile and immobile Gram-negative bacteria with ellipsoidal rod-shaped colonies that clustered in pairs and in isolated chains. These strains are capable of producing acetic acid from ethanol, growing on peptone and oxidizing acetate to CO2 and H2O. Strains FAGD1, FAGD10 and FAGD18 show negative growth on YPG medium containing D-glucose > 30%, while strains GCM2, GCM4 and GCM15 show positive growth. These six strains stand out on CARR indicator medium as isolates of the genus Acetobacter ssp. Analysis of 16S rDNA gene sequencing allowed us to differentiate these strains as Acetobacter fabarum and Acetobacter pasteurianus. The study of the tolerance of these six isolates towards pH showed that most of the six strains are unable to grow at pH 3 and pH 9, with an ideal pH of 5. The behavior of the six strains at different concentrations of ethanol shows an optimal production of acetic acid after incubation at concentrations between 6% and 8% (v/v) of ethanol. All six strains tolerated an ethanol concentration of 16% (v/v). The resistance of the strains to acetic acid differs between the species of AAB. The optimum acetic acid production is obtained at a concentration of 1% (v/v) for the strains of FAGD1, FAGD10 and FAGD18, and 3% (v/v) for GCM2, GCM4 and GCM15. These strains are able to tolerate an acetic acid concentration of up to 6% (v/v). The production kinetics of the six strains show the highest levels of growth and acetic acid production at 30 °C. This rate of growth and acetic acid production is high at 35 °C, 37 °C and 40 °C. Above 40 °C, the production of acid is reduced. All six strains continue to produce acetic acid, even at high temperatures up to 48 °C. These strains can be used in the vinegar production industry to minimize the load on cooling systems, especially in countries with high summer temperatures.
Homologous and Heterologous Expression of Delta(12)-Desaturase in Mucor circinelloides Enhanced the Production of Linolenic Acid
Authors: Yang Junhuan , Wang Xiuwen , Mohamed Hassan , Li Shaoqi , Wu Chen , Shi Wenyue , Xue Futing , López-García Sergio , Song Yuanda
Published: August 2022

Linolenic acid (LA) is gaining more interest within the scientific community. This is because it has a potential medical role in reducing the risk of inflammation, carcinogenesis, atherosclerosis and diabetes and is a valuable nutraceutical for human health. The oleaginous fungus Mucor circinelloides produces a high lipid content (36%), including valuable polyunsaturated fatty acids (PUFAs). However, the critical step in which oleic acid (OA) is converted into LA is not efficient at supplying enough substrates for PUFA synthesis. Hence, we propose a method to increase LA production based on genetic engineering. The overexpression of the Δ12-desaturase gene from M. circinelloides and Mortierella alpina increased the LA content and improved the lipid accumulation (from 14.9% to 21.6% in the Δ12-desaturase gene of the M. circinelloides overexpressing strain (Mc-D12MC) and from 14.9% to 18.7% in the Δ12-desaturase gene of M. alpina overexpressing strain (Mc-D12MA)). Additionally, the up-regulated expression levels of these genes targeted the genes involved in NADPH production, implying that the elevated Δ12-desaturase gene may function as a critical regulator of NADPH and lipid synthesis in M. circinelloides. This study provides the first evidence to support the design of metabolic engineering related to LA and PUFA production in M. circinelloides for potential industrial applications.
Gene Ontology (GO)-Driven Inference of Candidate Proteomic Markers Associated with Muscle Atrophy Conditions
Authors: Stalmach Angelique , Boehm Ines , Fernandes Marco , Rutter Alison , Skipworth Richard J. E. , Husi Holger
Published: August 2022

Skeletal muscle homeostasis is essential for the maintenance of a healthy and active lifestyle. Imbalance in muscle homeostasis has significant consequences such as atrophy, loss of muscle mass, and progressive loss of functions. Aging-related muscle wasting, sarcopenia, and atrophy as a consequence of disease, such as cachexia, reduce the quality of life, increase morbidity and result in an overall poor prognosis. Investigating the muscle proteome related to muscle atrophy diseases has a great potential for diagnostic medicine to identify (i) potential protein biomarkers, and (ii) biological processes and functions common or unique to muscle wasting, cachexia, sarcopenia, and aging alone. We conducted a meta-analysis using gene ontology (GO) analysis of 24 human proteomic studies using tissue samples (skeletal muscle and adipose biopsies) and/or biofluids (serum, plasma, urine). Whilst there were few similarities in protein directionality across studies, biological processes common to conditions were identified. Here we demonstrate that the GO analysis of published human proteomics data can identify processes not revealed by single studies. We recommend the integration of proteomics data from tissue samples and biofluids to yield a comprehensive overview of the human skeletal muscle proteome. This will facilitate the identification of biomarkers and potential pathways of muscle-wasting conditions for use in clinics.
Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples
Authors: Duarte Rodolfo Santos , Antunes Elisa Ribeiro Miranda , Sawaya Alexandra Christine Helena Frankland
Published: August 2022

Due to the widespread use of Maytenus ilicifolia leaves in the treatment of gastric ulcers, herbal medicines derived from such species are distributed by the national health system in Brazil. A related species, Maytenus aquifolium, is also used for the same disorders, and both are popularly known as Espinheira-santa. Due to their popular use, the quality and efficiency of the herbal medicines derived from these species is an important public health issue. The purpose of this study was to develop and test an analytical method that could quantify the content of catechin and epicatechin in dry Maytenus spp. leaves and simultaneously obtain their chemical profile to determine authenticity of the leaf samples. Ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) was used to quantify these isomers, in the selected ion monitoring (SIM) mode, while simultaneously analyzing the extract in full-scan mode. This approach was successfully applied to the analysis of commercial and authentic samples of Maytenus spp. Fewer than half the samples presented the minimum epicatechin content of 2.8 mg per g of dry leaf mass, as specified in the 6th Brazilian Pharmacopoeia (2019) for M. ilicifolia. Furthermore, by using untargeted metabolomics, it was observed that the chemical profile of most the samples was not compatible with M. ilicifolia leaves, indicating the need for stricter quality control of this material. The method described herein could be used for this control; moreover, its concept could be adapted and used for an ample variety of medicinal plant products.
Flower Extracts from Ornamental Plants as Sources of Sunscreen Ingredients: Determination by In Vitro Methods of Photoprotective Efficacy, Antigenotoxicity and Safety
Authors: Fuentes Jorge Luis , Pedraza Barrera Carlos Adolfo , Villamizar Mantilla Diego Armando , Flórez González Silvia Juliana , Sierra Lady Johanna , Ocazionez Raquel Elvira , Stashenko Elena E.
Published: August 2022

Plants are sources of sunscreen ingredients that prevent cellular mutations involved in skin cancer and aging. This study investigated the sunscreen properties of the extracts from some ornamental plants growing in Colombia. The UV filter capability of the flower extracts obtained from Rosa centifolia L., Posoqueria latifolia (Rudge) Schult, and Ipomoea horsfalliae Hook. was examined. Photoprotection efficacies were evaluated using in vitro indices such as sun protection factor and critical wavelength. UVB antigenotoxicity estimates measured with the SOS Chromotest were also obtained. Extract cytotoxicity and genotoxicity were studied in human fibroblasts using the trypan blue exclusion and Comet assays, respectively. Major compounds of the promising flower extracts were identified by UHPLC–ESI+–Orbitrap–MS. The studied extracts showed high photoprotection efficacy and antigenotoxicity against UVB radiation, but only the P. latifolia extract showed broad-spectrum photoprotection at non-cytotoxic concentrations. The P. latifolia extract appeared to be safer for human fibroblast cells and the R. centifolia extract was shown to be moderately cytotoxic and genotoxic at the highest assayed concentrations. The I. horsfalliae extract was unequivocally cytotoxic and genotoxic. The major constituents of the promising extracts were as follows: chlorogenic acid, ecdysterone 20E, rhamnetin-rutinoside, cis-resveratrol-diglucoside, trans-resveratrol-diglucoside in P. latifolia; quercetin, quercetin-glucoside, quercetin-3-rhamnoside, kaempferol, kaempferol-3-glucoside, and kaempferol-rhamnoside in R. centifolia. The potential of the ornamental plants as sources of sunscreen ingredients was discussed.
QSTR Modeling to Find Relevant DFT Descriptors Related to the Toxicity of Carbamates
Authors: Acosta-Jiménez Emma H. , Zárate-Hernández Luis A. , Camacho-Mendoza Rosa L. , González-Montiel Simplicio , Alvarado-Rodríguez José G. , Gómez-Castro Carlos Z. , Pescador-Rojas Miriam , Meneses-Viveros Amilcar , Cruz-Borbolla Julián
Published: August 2022

Compounds containing carbamate moieties and their derivatives can generate serious public health threats and environmental problems due their high potential toxicity. In this study, a quantitative structure–toxicity relationship (QSTR) model has been developed by using one hundred seventy-eight carbamate derivatives whose toxicities in rats (oral administration) have been evaluated. The QSRT model was rigorously validated by using either tested or untested compounds falling within the applicability domain of the model. A structure-based evaluation by docking from a series of carbamates with acetylcholinesterase (AChE) was carried out. The toxicity of carbamates was predicted using physicochemical, structural, and quantum molecular descriptors employing a DFT approach. A statistical treatment was developed; the QSRT model showed a determination coefficient (R2) and a leave-one-out coefficient (Q2LOO) of 0.6584 and 0.6289, respectively.
Small Molecule Inhibitors for Hepatocellular Carcinoma: Advances and Challenges
Authors: Kamal Monica A. , Mandour Yasmine M. , Abd El-Aziz Mostafa K. , Stein Ulrike , El Tayebi Hend M.
Published: August 2022

According to data provided by World Health Organization, hepatocellular carcinoma (HCC) is the sixth most common cause of deaths due to cancer worldwide. Tremendous progress has been achieved over the last 10 years developing novel agents for HCC treatment, including small-molecule kinase inhibitors. Several small molecule inhibitors currently form the core of HCC treatment due to their versatility since they would be more easily absorbed and have higher oral bioavailability, thus easier to formulate and administer to patients. In addition, they can be altered structurally to have greater volumes of distribution, allowing them to block extravascular molecular targets and to accumulate in a high concentration in the tumor microenvironment. Moreover, they can be designed to have shortened half-lives to control for immune-related adverse events. Most importantly, they would spare patients, healthcare institutions, and society as a whole from the burden of high drug costs. The present review provides an overview of the pharmaceutical compounds that are licensed for HCC treatment and other emerging compounds that are still investigated in preclinical and clinical trials. These molecules are targeting different molecular targets and pathways that are proven to be involved in the pathogenesis of the disease.
Biological Activities and Secondary Metabolites from Sophora tonkinensis and Its Endophytic Fungi
Authors: Liang Jia-Jun , Zhang Pan-Pan , Zhang Wei , Song Da , Wei Xin , Yin Xin , Zhou Yong-Qiang , Pu Xiang , Zhou Ying
Published: August 2022

The roots of Sophora tonkinensis Gagnep., a traditional Chinese medicine, is known as Shan Dou Gen in the Miao ethnopharmacy. A large number of previous studies have suggested the usage of S. tonkinensis in the folk treatment of lung, stomach, and throat diseases, and the roots of S. tonkinensis have been produced as Chinese patent medicines to treat related diseases. Existing phytochemical works reported more than 300 compounds from different parts and the endophytic fungi of S. tonkinensis. Some of the isolated extracts and monomer compounds from S. tonkinensis have been proved to exhibit diverse biological activities, including anti-tumor, anti-inflammatory, antibacterial, antiviral, and so on. The research progress on the phytochemistry and pharmacological activities of S. tonkinensis have been systematically summarized, which may be useful for its further research.
Baobab-Fruit Shell and Fibrous Filaments Are Sources of Antioxidant Dietary Fibers
Authors: Chiacchio Manuela Flavia , Tagliamonte Silvia , Visconti Attilio , Ferracane Rosalia , Mustafa Arwa , Vitaglione Paola
Published: August 2022

Since 2008, baobab-fruit dried pulp is listed as an ingredient on the European Union′s Novel Food Catalogue. By pulp production, 80% of the baobab fruit is discarded, forming side streams, namely, shell, fibrous filaments, and seeds. This study explored pulp and side-stream functional properties, including total dietary fiber (TDF), total antioxidant capacity (TAC), polyphenols, and water- (WHC) and oil-holding capacities (OHC), along with endocannabinoids (ECs) and N-acylethanolamines (NAEs) in pulp, seeds, and seed oil. Shell excelled in TDF (85%), followed by fibrous filaments (79%), and showed the highest soluble and direct TAC (72 ± 0.7 and 525 ± 1.0 µmol eq. Trolox/g, respectively). Pulp was the richest in polyphenols, followed by shell, fibrous filaments, and seeds. Quercetin predominated in shell (438.7 ± 2.5 µg/g); whereas epicatechin predominated in pulp (514 ± 5.7 µg/g), fibrous filaments (197.2 ± 0.1 µg/g), and seeds (120.1 ± 0.6 µg/g); followed by procyanidin B2 that accounted for 26–40% of total polyphenols in all the products. WHC and OHC ranged between 2–7 g H2O-Oil/g, with fibrous filaments showing the highest values. ECs were not found, whereas NAEs were abundant in seed oil (2408.7 ± 11.1 ng/g). Baobab shell and fibrous filaments are sources of polyphenols and antioxidant dietary fibers, which support their use as functional food ingredients.
Nano-Sheet-like Morphology of Nitrogen-Doped Graphene-Oxide-Grafted Manganese Oxide and Polypyrrole Composite for Chemical Warfare Agent Simulant Detection
Authors: Lama Sanjeeb , Bae Bong-Gyu , Ramesh Sivalingam , Lee Young-Jun , Kim Namjin , Kim Joo-Hyung
Published: August 2022

Chemical warfare agents (CWAs) have inflicted monumental damage to human lives from World War I to modern warfare in the form of armed conflict, terrorist attacks, and civil wars. Is it possible to detect the CWAs early and prevent the loss of human lives? To answer this research question, we synthesized hybrid composite materials to sense CWAs using hydrothermal and thermal reduction processes. The synthesized hybrid composite materials were evaluated with quartz crystal microbalance (QCM) and surface acoustic wave (SAW) sensors as detectors. The main findings from this study are: (1) For a low dimethyl methyl phosphonate (DMMP) concentration of 25 ppm, manganese dioxide nitrogen-doped graphene oxide (NGO@MnO2) and NGO@MnO2/Polypyrrole (PPy) showed the sensitivities of 7 and 51 Hz for the QCM sensor and 146 and 98 Hz for the SAW sensor. (2) NGO@MnO2 and NGO@MnO2/PPy showed sensitivities of more than 50-fold in the QCM sensor and 100-fold in the SAW sensor between DMMP and potential interferences. (3) NGO@MnO2 and NGO@MnO2/PPy showed coefficients of determination (R2) of 0.992 and 0.975 for the QCM sensor and 0.979 and 0.989 for the SAW sensor. (4) NGO@MnO2 and NGO@MnO2/PPy showed repeatability of 7.00 ± 0.55 and 47.29 ± 2.69 Hz in the QCM sensor and 656.37 ± 73.96 and 665.83 ± 77.50 Hz in the SAW sensor. Based on these unique findings, we propose NGO@MnO2 and NGO@MnO2/PPy as potential candidate materials that could be used to detect CWAs.
Obesity-Associated Anxiety Is Prevalent among College Students and Alleviated by Calorie Restriction
Authors: Wang Junbo , Ran Xinyi , Ye Jinchen , Deng Run , Dang Weimin , Fan Yangyi , Hu Zhiping , Yang Lei , Dong Wentian , Lv Yifei , Lin Kunzhan , Li Maoran , Jiang Yuhe , Zheng Ruimao
Published: August 2022

Anxiety is a common disorder among college students, especially those with obesity. Obesity contributes to metabolic disorders and disturbs the neural functions, further leading to anxiety. In this cross-sectional study, we aimed to determine the association between obesity and anxiety among college students and identified the potential factors for obesity-associated anxiety. We evaluated the intervention effects of calorie restriction on anxiety. Self-reported questionnaires were distributed to 1381 college students from January to March in 2021. Anxiety was measured by the State-Trait Anxiety Inventory (STAI). Participants were classified into anxiety and non-anxiety groups according to their STAI scores. Chi-squared test and logistic regression were used to analyze the potential factors. We found that 383 college students exhibited anxiety, accounting for 30.1% among all included college students, which was higher than the global average. The association between anxiety and obesity was observed among college students (p = 0.009), especially in males (p = 0.007). We identified that pre-obesity (p = 0.012), unhealthy calorie intake (p = 0.001), dieting (p = 0.003) and high academic year (p = 0.006) as the risk factors for anxiety and found that the long sleep duration was a protective factor for anxiety (p < 0.001). We found that more obese students showed an improvement of anxiety than the underweight students after calorie restriction (p < 0.001). Collectively, our findings suggest that obesity-associated anxiety is prevalent among the college students and could be alleviated by moderate calorie restriction. It is necessary for students to receive anxiety management in their college life. Additionally, the proper calorie restriction should be promoted to help students protect against obesity and obesity-associated anxiety.