bluuebunny/crossref_metadata_2025 huggingface dataset:
Prognostic value of red blood cell distribution width-to-albumin ratio in ICU patients with coronary heart disease and diabetes mellitus
Authors: Chen Sheng , Guan Senhong , Yan Zhaohan , Ouyang Fengshan , Li Shuhuan , Liu Lanyuan , Zuo Liuer , Huang Yuli , Zhong Jiankai
Published: September 2024

Background

The red blood cell distribution width (RDW)-to-albumin ratio (RAR) has emerged as a potentially valuable prognostic indicator in diverse medical conditions. However, the prognostic significance of RAR in intensive care unit (ICU) patients with coronary heart disease (CHD) and diabetes mellitus (DM) remains uncertain and requires further investigation.

Methods

This study aims to investigate the prognostic significance of RAR in ICU patients with coexisting CHD and DM through a retrospective cohort analysis using data from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database (version 2.2). The study population included patients aged 18 years or older who were diagnosed with both CHD and DM. The primary endpoint was 1-year mortality, and the secondary endpoints included 30-day mortality, 90-day mortality, hospital length of stay (LOS), and ICU LOS.

Results

A total of 3416 patients, of whom 64.64% were male, were included in the study. The 30-day mortality, 90-day mortality, and 1-year mortality were 7.08%, 7.44%, and 7.49%, respectively. After adjusting for confounding factors, multivariate Cox proportional risk analysis demonstrated that high RAR levels were associated with an increased risk of 30-day mortality (HR, 1.53 [95% CI 1.17-2.07], P = 0.006), 90-day mortality (HR, 1.58 [95% CI 1.17-2.13], P = 0.003), and 1-year mortality (HR, 1.58 [95% CI 1.17-2.13], P = 0.003). Furthermore, the restricted cubic spline (RCS) model indicated a linear relationship between RAR and 1-year mortality.

Conclusion

The results suggest that RAR holds potential as a valuable prognostic biomarker in ICU patients with both CHD and DM. Elevated RAR levels were found to be significantly associated with increased mortality during hospitalization, facilitating the identification of individuals at higher risk of adverse outcomes. These findings underscore the importance of incorporating RAR into risk stratification and overall management strategies for ICU patients with coexisting CHD and DM.
Prevalence of chronic postsurgical hypoparathyroidism not adequately controlled: an analysis of a nationwide cohort of 337 patients
Authors: Díez Juan J. , Anda Emma , Pérez-Corral Begoña , Paja Miguel , Alcazar Victoria , Sánchez-Ragnarsson Cecilia , Orois Aida , Romero-Lluch Ana R. , Sambo Marcel , Oleaga Amelia , Caballero Águeda , Alhambra María R. , Urquijo Virginia , Delgado-Lucio Ana M. , Fernández-García José C. , Doulatram-Gamgaram Viyey K. , Dueñas-Disotuar Suset , Martín Tomás , Peinado Mercedes , Sastre Julia
Published: September 2024

Purpose

The identification of patients with chronic hypoparathyroidism who are adequately (AC) or not adequately controlled (NAC) has clinical interest, since poor disease control is related to complications and mortality. We aimed to assess the prevalence of NAC patients in a cohort of subjects with postsurgical hypoparathyroidism.

Methods

We performed a multicenter, retrospective, cohort study including patients from 16 Spanish hospitals with chronic hypoparathyroidism lasting ≥3 years. We analyzed disease control including biochemical profile and clinical wellness. For biochemical assessment we considered three criteria: criterion 1, normal serum calcium, phosphorus and calcium x phosphorus product; criterion 2, the above plus estimated glomerular filtration rate ≥60 ml/min/1.73 m2; and criterion 3, the above plus normal 24-hour urinary calcium excretion. A patient was considered AC if he or she met the biochemical criteria and was clinically well.

Results

We included 337 patients with postsurgical hypoparathyroidism (84.3% women, median age 45[36-56] years, median time of follow-up 8.9[6.0-13.0] years). The proportions of NAC patients with criteria 1, 2 and 3 were, respectively, 45.9%, 49.2% and 63.1%. Patients who had dyslipidemia at the time of diagnosis presented a significantly higher risk of NAC disease (criterion 3; OR 7.05[1.44-34.45]; P=0.016). NAC patients (criterion 2) had a higher proportion of subjects with incident chronic kidney disease and eye disorders, and NAC patients (criterion 3) had a higher proportion of incident chronic kidney disease, nephrolithiasis and dyslipidemia than AC patients.

Conclusion

The present study shows a strikingly high prevalence of NAC patients in the clinical practice of Spanish endocrinologists. Results suggest that NAC disease might be associated with some prevalent and incident comorbidities.
Survival of plant seeds in digestate storage—with and without prior anaerobic digestion
Authors: Hahn Juliane , Plogsties Vincent , Gerowitt Bärbel , Heiermann Monika
Published: September 2024

Introduction

Plant seeds from weeds and energy crops have the potential to survive anaerobic digestion (AD). Species able to form physically dormant, i.e., hardseeded (HS) seeds seem particularly resistant. However, it is not yet known to what extent the storage of the digestate after AD affects seed viability.

Methods

Seed survival of five HS and six non-HS (NHS) species was investigated in three combinations of digestate storage (DS) and AD. First, untreated seeds were exposed to DS for maximum 12 weeks. To simulate short-circuited AD (ADshort) in biogas reactors, seeds were second subjected to lab-scale AD for 1 day before DS. Third, seeds of six species were exposed to full-scale AD (ADfull) followed by DS. Seed viability was determined using a combination of germination tests and tetrazolium staining. Viability was modeled as a function of exposure time.

Results and discussion

Seed viability was affected by DS, AD and AD + DS, but responses varied greatly between species and treatments. With increasing exposure time, viability decreased after a lag-phase, remained stable or even increased. The NHS species Cichorium intybus, Daucus carota, Echium vulgare, and Verbascum thapsus were most susceptible, with seed-killing close to 100% if DS was involved. The HS species Malva sylvestris, Melilotus albus and Melilotus officinalis were most resistant. They survived all treatments and were alive after 35 days of ADfull plus 3 months of DS. The resistance potential of the HS species Abutilon theophrasti and Malva alcea and of the NHS species Chenopodium album and two tomato varieties was intermediate. None of them survived ADfull + DS, but except A. theophrasti they were viable after ADshort + DS. With few exceptions, seed-killing by AD + DS was higher than that by AD alone. In conclusion, DS and AD + DS have the potential to reduce seed viability, but do not completely inactivate all species. Therefore, digestate can be contaminated with viable seeds and may lead to the spread of weeds, especially after a short-circuited AD. In order to ensure the sustainable use of digestates in terms of weeds, we recommend to investigate the factors contributing to seed inactivation and the quantity of seed introduced to AD.
The granular landscape of health disparities: a socio-spatial examination of chronic disease prevalence within New York City's community districts
Authors: Martin-Ikpe Cordelia , Were Lawrence P. O. , Okoror Titilayo A.
Published: September 2024

This study investigates the relationship between neighborhood Socioeconomic Position (SEP) and the prevalence of hypertension and coronary heart disease (CHD) across New York City's community districts. By using a composite SEP index derived from multiple socioeconomic indicators, the research seeks to uncover health disparities that may be obscured in broader geographic analyses. A primary objective is to identify community districts where hypertension and CHD prevalence is significantly higher than expected, even after adjusting for SEP. This focus helps pinpoint areas where targeted public health interventions may be urgently required, especially when the anticipated inverse relationship between SEP and health outcomes does not hold. A linear mixed-effects model (LMM) was used to analyze the association between SEP and these health outcomes, incorporating both fixed and random effects to account for variation across the city's 59 community districts. The analysis revealed a significant negative association between SEP and both hypertension and CHD, with higher SEP levels generally correlating with lower prevalence rates. However, some districts exhibited high random effects, indicating a higher-than-expected prevalence of these conditions, even after controlling for SEP. The authors suspect that additional factors-such as environmental exposures, healthcare accessibility, or community infrastructure-may contribute to these unexplained disparities. The findings emphasize the need for public health strategies that are sensitive to local contexts and the multifactorial nature of health inequities. Understanding the complex drivers behind these disparities can inform policies aimed at reducing health inequities in diverse urban environments.
Fostering fisheries futures: the promise of localized stewardship education in Nunatsiavut
Authors: Laver Quinna , Andersen Ola , Moore Sylvia , Pottle Colleen , Snook Jamie
Published: September 2024

Nunatsiavut is a self-governing Inuit territory in northern coastal Labrador, where cultural knowledge and social values are incorporated into co-managed subsistence and commercial fisheries. Snow crab (Chionoecetes opilio), known as Putjotik in Inuttitut (the Nunatsiavut dialect of Inuktitut), is a co-managed fishery through the Torngat Joint Fisheries Board (TJFB) and plays a crucial role in sustaining the community of Makkovik. Arctic Char (IKaluk) is also harvested in co-managed subsistence and commercial fisheries, and is a staple in local economies and culture. In 2022, the TJFB partnered with Inuit educators to foster youth stewardship in a project called Paigitsiaguk. The Paigitsiaguk project bridges Inuit and scientific knowledge to nurture cultural values and environmental stewardship through providing place and land-based resources to educators. The project team created a comprehensive database of curriculum outcomes required by the Newfoundland and Labrador school system, and then designed culturally relevant learning activities that presented Inuit knowledge with science and social studies for students in Nunatsiavut. These resources include learning activities that are organized into education kits: one of which is centered on Putjotik and another on IKaluk. The Putjotik kit has 23 activities tailored to meet grade-specific learning outcomes, while the IKaluk kit provides 12 place-based learning activities appropriate for multiple grade levels. By providing localized and culturally relevant education resources to teachers that revolve around stewarding local fish species and their ecosystems, youth are connecting to their communities and to the coastal environment. Educational resources and learning activities reflect Inuit and scientific knowledge systems, and position teachers as facilitators to encourage students to learn from the land and Elders, knowledge holders, fishers, scientists, and managers. This paper reflects the promising futures that this work can contribute to; from facilitating intergenerational knowledge transmission from knowledge holders to youth, to encouraging self-determined stewardship activities that encourage care for the ocean and its fisheries.
Characteristics and risk assessments of mercury pollution in a county region undergoing industrialization in northeastern China: a case study in Gongzhuling
Authors: Zhang Tingting , Hu Yufei , Wu Jing , Yin Shizhong , Huang Jia , Huang Wang , Huang Hongjin , Zhang Gang
Published: September 2024

Mercury pollution in emerging industrial zones and surrounding areas, especially in industrial concentration areas, has attracted much attention. So as to clarify the characteristics of Hg in the environment in China’s small and medium-sized industrial emerging and surrounding areas, central urban area of Gongzhuling, a traditional agricultural town with a high degree of industrialization in northeast Changchun industrial base, is taken as the scope. Geological accumulation trin (Igeo) was used to study the degree of soil mercury contamination in Gongzhuling area, latent Er (ecological risk) trin was used to appraise the Er of soil Hg in the study area, and non-carcinogenic risk assessment of mercury in soil using human exposure risk assessment model. The results showed that 34% of soil samples had higher Hg content than the background value of the province’s soil (0.04 mg kg−1). The Igeo results showed that Hg pollution rate of soil in study area was 9% (index >0). In conclusion, the level of soil Hg pollution in Gongzhuling area was low, and the pollution area is mainly concentrated in the northwestern part of the study range. The highest and lowest Er values of soil Hg from the study sample were 2.23 and 214.83, and 24% of the samples had Er > 40, that means they pose a moderate or higher potential ecological risk, and most of these points are located in the northeast of the study range. The main route of human exposure to Hg is oral ingestion. The HQ (non-carcinogenic risk index) and HI (total non-carcinogenic risk value) of soil mercury were both much less than 1, it indicates that the present level of soil Hg in the study range does not pose a threat to local adult health for the time being. This study provides reference for other urban pollution risk assessment, and further defines the direction of future work.
Comprehensive assessment of the earth’s surface state disturbed by mining and ways to improve the situation: case study of Kryvyi Rih Iron-ore Basin, Ukraine
Authors: Bazaluk Oleg , Petlovanyi Mykhailo , Sai Kateryna , Chebanov Maksym , Lozynskyi Vasyl
Published: September 2024

Introduction

Complex mineral mining leads to critical earth’s surface disturbance and environmental pollution from industrial waste. This research aims to comprehensively study, assess and show the current scale of the earth’s surface disturbances in the largest mining region of Ukraine – the Kryvyi Rih. Iron-ore Basin and suggests ways to improve the situation based on backfill technologies for the earth’s surface rehabilitation and industrial waste utilization.

Methods

To identify the forms of surface disturbance, their parameters, and spatial location, satellite images of the region, government statistical data, and a geographic information system software suite were studied. An analysis of the effectiveness of existing reclamation measures for technogenic voids in the region was carried out, and a new concept of surface restoration was proposed using beneficiation tailings, which are difficult to utilize in the region.

Results

It has been determined that the contribution of the Kryvyi Rih Iron-ore Basin to waste generation is 92.08% of the total volume of waste accumulations in Dnipropetrovsk Oblast and 65.5% in Ukraine as a whole, amounting to 10.7 billion tons. It is determined that 65.25% of the total disturbed land area is industrial waste, 34.76% – quarry cavities and mine failure zones, occupying 49% of the Kryvyi Rih city area. An analysis of waste accumulation density in allotted areas shows that per 1 m2 of land area, on average, 183.5 tons of dump waste rocks and 73.5 tons of beneficiation tailings are placed. Disposal of beneficiation tailings is recognized as a priority, because their utilization is slower, occupy the largest area, creating a risk of dams breaching and intense dusting from tailings dams surface, threatening the environment and public health. For the first time in Ukraine, a concept for the earth’s surface rehabilitation for various industrial purposes has been developed based on paste backfill technologies.

Discussion

Paste backfill technology implementation is prioritized in the central and northern parts of the city, which can dispose of at least 65–70 million tons of waste in cavities and reclaim about 200 ha of land. The research results are valuable for the development of strategies or programs to overcome the technogenic-ecological crisis in the city. The rehabilitated earth’s surface can be usefully involved in various infrastructure projects for the development of the industrial and economic potential of an industrial city such as Kryvyi Rih.
The complete chloroplast genome of Psydrax latifolia: evolutionary dynamics, comparative genomics and phylogeny
Authors: Safhi Fatmah Ahmed , Jalal Areej Saud , Alshegaihi Rana M. , Alshamrani Rahma , Alamri Amnah M. , Felemban Wessam , Abuzaid Amani Omar , Hussein Mohammed A. A. , Al Aboud Nora M. , Magdy Mahmoud , Abd El-Moneim Diaa
Published: September 2024

Introduction

This study presents the first complete plastome (cpDNA) sequence of Psydrax latifolia, a member of the Rubiaceae family, which includes small trees, smooth shrubs, and occasionally lianas. This specimen was collected near the Red Sea coast in Jazan province, Saudi Arabia, specifically in the paleotropical Fifa Mountains. The research aimed to characterize and compare the plastome of Psydrax latifolia with other species in the Rubiaceae family to enhance our understanding of its evolutionary dynamics and phylogenetic relationships.

Methods

The plastome of P. latifolia was sequenced and reconstructed using whole-genome next-generation sequencing (NGS) techniques. Comparative analyses were performed between the plastome of P. latifolia and 16 other species within the Rubiaceae family to identify genomic features and evolutionary patterns. The plastome structure, gene content, and codon usage were analyzed, with a focus on the Relative Synonymous Codon Usage (RSCU) in different regions of the plastome.

Results

The plastome of P. latifolia was found to be 153,242 base pairs (bp) in length, including a large single copy (LSC) region of 83,603 bp, a small single copy (SSC) region of 18,115 bp, and a pair of inverted repeats (IRs) of 25,762 bp each. It contained 87 protein-coding genes, 8 rRNA genes, and 33 tRNA genes, with an overall GC content of 37.30%. The RSCU analysis revealed regional variation, with the protein-coding region being more conserved than the intergenic spacer.

Discussion

This study provides the first complete plastome sequence of Psydrax latifolia, offering insights into its genomic structure and phylogenetic position within the Rubiaceae family. Comparative analyses with 16 Rubiaceae species highlighted distinct genomic features and evolutionary patterns. These findings contribute to the understanding of plastome evolution in the Rubiaceae family and provide a valuable resource for future phylogenetic and evolutionary studies.
Integrating transcriptomics, eQTL, and Mendelian randomization to dissect monocyte roles in severe COVID-19 and gout flare
Authors: Li Jiajia , Yang Guixian , Liu Junnan , Li Guofeng , Zhou Huiling , He Yuan , Fei Xinru , Zhao Dongkai
Published: September 2024

Introduction

There are considerable similarities between the pathophysiology of gout flare and the dysregulated inflammatory response in severe COVID-19 infection. Monocytes are the key immune cells involved in the pathogenesis of both diseases. Therefore, it is critical to elucidate the molecular basis of the function of monocytes in gout and COVID-19 in order to develop more effective therapeutic approaches.

Methods

The single-cell RNA sequencing (scRNA-seq), large-scale genome-wide association studies (GWAS), and expression quantitative trait loci (eQTL) data of gout and severe COVID-19 were comprehensively analyzed. Cellular heterogeneity and intercellular communication were identified using the scRNA-seq datasets, and the monocyte-specific differentially expressed genes (DEGs) between COVID-19, gout and normal subjects were screened. In addition, the correlation of the DEGs with severe COVID-19 and gout flare was analyzed through GWAS statistics and eQTL data.

Results

The scRNA-seq analysis exhibited that the proportion of classical monocytes was increased in both severe COVID-19 and gout patient groups compared to healthy controls. Differential expression analysis and MR analysis showed that NLRP3 was positively associated with the risk of severe COVID-19 and involved 11 SNPs, of which rs4925547 was not significantly co-localized. In contrast, IER3 was positively associated with the risk of gout and involved 9 SNPs, of which rs1264372 was significantly co-localized.

Discussion

Monocytes have a complex role in gout flare and severe COVID-19, which underscores the potential mechanisms and clinical significance of the interaction between the two diseases.
Whole-genome sequencing reveals rare variants associated with gout in Taiwanese males
Authors: Tseng Yu-Ping , Chang Ya-Sian , Mekala Venugopala R. , Liu Ting-Yuan , Chang Jan-Gowth , Shieh Grace S.
Published: September 2024

To identify rare variants (RVs) of gout, we sequenced the whole genomes of 321 male gout patients and combined these with those of 64 male gout patients and 682 normal controls at Taiwan Biobank. We performed ACAT-O to identify 682 significant RVs (p < 3.8 × 10−8) clustered on chromosomes 1, 7, 10, 16, and 18. To prioritize causal variants effectively, we sifted them by Combined Annotation-Dependent Depletion score >10 or |effect size| ≥ 1.5 for those without CADD scores. In particular, to the best of our knowledge, we identified the rare variants rs559954634, rs186763678, and 13-85340782-G-A for the first time to be associated with gout in Taiwanese males. Importantly, the RV rs559954634 positively affects gout, and its neighboring gene NPHS2 is involved in serum urate and expressed in kidney tissues. The kidneys play a major role in regulating uric acid levels. This suggests that rs559954634 may be involved in gout. Furthermore, rs186763678 is in the intron of NFIA that interacts with SLC2A9, which has the most significant effect on serum urate. Note that gene-gene interaction NFIA-SLC2A9 is significantly associated with serum urate in the Italian MICROS population and a Croatian population. Moreover, 13-85340782-G-A significantly affects gout susceptibility (odds ratio 6.0; P = 0.038). The >1% carrier frequencies of these potentially pathogenic (protective) RVs in cases (controls) suggest the revealed associations may be true; these RVs deserve further studies for the mechanism. Finally, multivariate logistic regression analysis shows that the rare variants rs559954634 and 13-85340782-G-A jointly are significantly associated with gout susceptibility.
Higher frequency of peripheral blood CD103+CD8+ T cells with lower levels of PD-1 and TIGIT expression related to favorable outcomes in leukemia patients
Authors: Liu Lian , Lai Wenpu , Zhuo Xiaoling , Chen Sihui , Luo Xiaodan , Tan Huo
Published: September 2024

Background

Leukemia is a prevalent pediatric life-threatening hematologic malignancy with a poor prognosis. Targeting immune checkpoints (ICs) to reverse T cell exhaustion is a potentially effective treatment for leukemia. Tissue resident memory T (TRM) cells have been found to predict the efficacy of programmed death receptor-1 inhibitor (anti-PD-1) therapy in solid tumors. However, the IC characteristics of TRM cells in leukemia and their relationship with prognosis remain unclear.

Methods

We employed multi-color flow cytometry to evaluate the frequencies of CD103+CD4+ and CD103+CD8+ T cells in the peripheral blood (PB) of patients with acute myeloid leukemia and B-cell acute lymphoblastic leukemia compared to healthy individuals. We examined the expression patterns of PD-1 and T cell immunoreceptor with immunoglobulin and ITIM domain (TIGIT) within the circulating CD103+ T cell subsets affected by leukemia. To further elucidate the immunological landscape, we assessed the differentiation status of CD103+ T cells across various disease states in patients with leukemia.

Results

Our findings showed a significant increase in the frequency of CD103+CD8+ T cells in the PB of patients with leukemia who had achieved complete remission (CR) compared to those in the de novo (DN) and relapsed/refractory (RR) stages. This increase was accompanied by a notable decrease in the expression levels of PD-1 and TIGIT in CD103+CD8+ T cells in the CR stage. Additionally, our analysis revealed a higher proportion of CD103+CD8+ T cells in the central memory (TCM) and effector memory (TEM) subsets of the immune profile. Notably, the proportions of CD103+ naïve T cells, CD103+ TEM, and CD103+ terminally differentiated T cells within the CD8+ T cell population were significantly elevated in patients with CR compared to those in the DN/RR stages.

Conclusion

The data indicate that circulating higher frequency of CD103+CD8+ T cells with lower expression of PD-1 and TIGIT are associated with favorable outcomes in patients with leukemia. This suggests a potential role of TRM cells in leukemia prognosis and provides a foundation for developing targeted immunotherapies.
Differential expression of HIV target cells CCR5 and α4β7 in tissue resident memory CD4 T cells in endocervix during the menstrual cycle of HIV seronegative women
Authors: Govindaraj Sakthivel , Tyree Staple , Herring Gina Bailey , Rahman Sadia J. , Babu Hemalatha , Ibegbu Chris , Young Marisa R. , Mehta C. Christina , Haddad Lisa B. , Smith Alicia K. , Velu Vijayakumar
Published: September 2024

Background

Ovarian hormones are known to modulate the immune system in the female genital tract (FGT). We sought to define the impact of the menstrual cycle on the mucosal HIV target cell levels, and tissue-resident CD4 T cells.

Materials and methods

Here, we characterized the distribution, phenotype, and function of CD4 T cells with special emphasis on HIV target cells (CCR5+ and α4β7+) as well as tissue-resident memory (TRM; CD69+ and CD103+) CD4 T cells in FGT of cycling women. Peripheral blood and Endocervical cells (EC-collected from cytobrush) were collected from 105 healthy women and performed multicolor flow cytometry to characterize the various subsets of CD4 T cells. Cervicovaginal lavage (CVL) were collected for cytokine analysis and plasma were collected for hormonal analysis. All parameters were compared between follicular and luteal phase of menstrual cycle.

Results

Our findings revealed no significant difference in the blood CD4 T cell subsets between the follicular and luteal phase. However, in EC, the proportion of several cell types was higher in the follicular phase compared to the luteal phase of menstrual cycle, including CCR5+α4β7-cells (p=0.01), CD69+CD103+ TRM (p=0.02), CCR5+CD69+CD103+ TRM (p=0.001) and FoxP3+ CD4 T cells (p=0.0005). In contrast, α4β7+ CCR5- cells were higher in the luteal phase (p=0.0004) compared to the follicular phase. In addition, we also found that hormonal levels (P4/E2 ratio) and cytokines (IL-5 and IL-6) were correlated with CCR5+ CD4 T cells subsets during the follicular phase of the menstrual cycle

Conclusion

Overall, these findings suggest the difference in the expression of CCR5 and α4β7 in TRM CD4 T cell subsets in endocervix of HIV seronegative women between the follicular and luteal phase. Increase in the CCR5+ expression on TRM subsets could increase susceptibility to HIV infection during follicular phase of the menstrual cycle.
Cross-omics strategies and personalised options for lung cancer immunotherapy
Authors: Yan Yalan , Shen Siyi , Li Jiamin , Su Lanqian , Wang Binbin , Zhang Jinghan , Lu Jiaan , Luo Huiyan , Han Ping , Xu Ke , Shen Xiang , Huang Shangke
Published: September 2024

Lung cancer is one of the most common malignant tumours worldwide and its high mortality rate makes it a leading cause of cancer-related deaths. To address this daunting challenge, we need a comprehensive understanding of the pathogenesis and progression of lung cancer in order to adopt more effective therapeutic strategies. In this regard, integrating multi-omics data of the lung provides a highly promising avenue. Multi-omics approaches such as genomics, transcriptomics, proteomics, and metabolomics have become key tools in the study of lung cancer. The application of these methods not only helps to resolve the immunotherapeutic mechanisms of lung cancer, but also provides a theoretical basis for the development of personalised treatment plans. By integrating multi-omics, we have gained a more comprehensive understanding of the process of lung cancer development and progression, and discovered potential immunotherapy targets. This review summarises the studies on multi-omics and immunology in lung cancer, and explores the application of these studies in early diagnosis, treatment selection and prognostic assessment of lung cancer, with the aim of providing more personalised and effective treatment options for lung cancer patients.
Transformation of macrophages into myofibroblasts in fibrosis-related diseases: emerging biological concepts and potential mechanism
Authors: Li Xiujun , Liu Yuyan , Tang Yongjun , Xia Zhaoyi
Published: September 2024

Macrophage-myofibroblast transformation (MMT) transforms macrophages into myofibroblasts in a specific inflammation or injury microenvironment. MMT is an essential biological process in fibrosis-related diseases involving the lung, heart, kidney, liver, skeletal muscle, and other organs and tissues. This process consists of interacting with various cells and molecules and activating different signal transduction pathways. This review deeply discussed the molecular mechanism of MMT, clarified crucial signal pathways, multiple cytokines, and growth factors, and formed a complex regulatory network. Significantly, the critical role of transforming growth factor-β (TGF-β) and its downstream signaling pathways in this process were clarified. Furthermore, we discussed the significance of MMT in physiological and pathological conditions, such as pulmonary fibrosis and cardiac fibrosis. This review provides a new perspective for understanding the interaction between macrophages and myofibroblasts and new strategies and targets for the prevention and treatment of MMT in fibrotic diseases.
Ataxic sensory neuronopathy with isolated anti-Ro/SS-A antibody during pregnancy: a case report of adverse outcomes
Authors: Smaili Rachid , Ferjouchia Zahra , Elbachiri Soumaya , Bourkia Myriem
Published: September 2024

We present the rare case of a 33-year-old pregnant woman who developed severe ataxic sensory neuronopathy associated with isolated anti-Ro/SS-A antibodies without other features of Sjögren's syndrome. Her symptoms, which began with numbness and paresthesia, progressed to severe ataxia and sensory impairment, complicated by preeclampsia and intrauterine fetal death. Despite treatment with intravenous immunoglobulin, azathioprine, and steroids, she remains disabled. This case raises awareness for anti-Ro/SS-A antibodies in pregnant women and underscores the importance of early intervention and a multidisciplinary approach to prevent severe neurological disability.
Strength characteristics of cement stabilized construction waste slurry modified by polyacrylamide with different moisture contents
Authors: Guo Feng , Hu Jiabin
Published: September 2024

Waste slurry is a major component of construction waste, and its resource utilization can effectively reduce its environmental impact. The effect of polyacrylamide (PAM) content and moisture content on the strength characteristics of PAM modified cement stabilized construction waste slurry (PCMS) was studied using unconfined compressive strength (UCS) and triaxial tests. It can be concluded that, 1) The UCS of PCMS increases with the increase of curing age and significantly decreases with the increase of moisture content. As the content of PAM increases, it first increases and then decreases, with UCS reaching its maximum at a PAM content of 0.5%. 2) When the moisture content is 50%, PAM can increase the elastic modulus of PCMS. When the content of PAM is 0.5%, the elastic modulus reaches its maximum value. When the moisture content is 80% and 100%, the effect of PAM on the elastic modulus of PCMS is not significant. 3) The addition of PAM can improve the shear strength of PCMS. Under the same confining pressure, the shear strength of PCMS increases first and then decreases with the increase of PAM content, and the optimal content is 0.5%. 4) The variation pattern of PCMS cohesion is basically consistent with the shear strength. PAM improves the shear strength of PCMS by enhancing its cohesion. The addition of PAM has a relatively small impact on the internal friction angle of PCMS. These findings provide valuable insights for research into modification technology and the resource utilization of construction waste slurry.
Regulation of idiopathic pulmonary fibrosis: a cross-talk between TGF-β signaling and MicroRNAs
Authors: Wang Shuo , Yu Hanming , Liu Shi , Liu Yuqing , Gu Xiu
Published: September 2024

Pulmonary fibrosis (PF) is a highly complex and challenging disease affecting the respiratory system. Patients with PF usually have an abbreviated survival period and a consequential high mortality rate after the diagnosis is confirmed, posing serious threats to human health. In clinical practice, PF is typically treated by antifibrotic agents, such as Pirfenidone and Nintedanib. However, these agents have been reported to correlate with substantial adverse effects, escalating costs, and insufficient efficacy. Moreover, it remains unclarified about the multifactorial pathology of PF. Therefore, there is an urgent demand for elucidating these underlying mechanisms and identifying safe, efficient, and targeted therapeutic strategies for PF treatment. The crucial role of the transforming growth factor-β (TGF-β) signaling pathway in PF development has been explored in many studies. MicroRNAs (miRNAs), which function as post-transcriptional regulators of gene expression, can significantly affect the development of PF by modulating TGF-β signaling. In turn, TGF-β signaling can regulate the expression and biogenesis of miRNAs, thereby substantially affecting the progression of PF. Hence, the therapeutic strategies that focus on the drug-targeted regulation of miRNAs, either by augmenting down-regulated miRNAs or inhibiting overexpressed miRNAs, may hinder the pathways related to TGF-β signaling. These strategies may contribute to the prevention and suppression of PF progression and may provide novel insights into the treatment of this disease.
Ethereum blockchain for electronic health records: securing and streamlining patient management
Authors: Mole J. S. Simi , Shaji R. S.
Published: September 2024

Electronic health records (EHRs) are increasingly replacing traditional paper-based medical records due to their speed, security, and ability to eliminate redundant data. However, challenges such as EHR interoperability and privacy concerns remain unresolved. Blockchain, a distributed ledger technology comprising connected, encrypted data blocks, presents a promising solution. This study explores how blockchain technology can revolutionize hospital EHR management. Our proposed solution securely transfers medical records between patients and doctors using the InterPlanetary File System (IPFS) and the Ethereum platform. Utilizing smart contracts automates data transfers, ensuring patient anonymity and reducing computational complexity while securely storing patient data on the network. Patient records are stored locally on the Ganache server, with the front end managed using HTML, CSS, ReactJS, and JavaScript, and the backend developed in Solidity. Blockchain technologies combined with Role- Based access control instead of attribute -based access control. The system’s throughput increases linearly with the number of users and requests, enhancing the framework’s efficiency and scalability. The minimum recorded latency is 14 ms.
Prostate-specific membrane antigen PET versus [99mTc]Tc-MDP bone scan for diagnosing bone metastasis in prostate cancer: a head-to-head comparative meta-analysis
Authors: Wang Yiming , Qiu Yiran , Yan Xingjian
Published: September 2024

Purpose

To evaluate the diagnostic performance of PSMA PET/CT, including [68Ga]Ga-PSMA-11 and [18F]DCFPyL, in comparison with the [99mTc]Tc-MDP bone scan (BS) in identifying bone metastases among prostate cancer patients.

Methods

A search was performed in the PubMed and Embase databases to locate pertinent publications from inception to February 12, 2024. The studies included were those that examined the diagnostic effectiveness of PSMA PET/CT (covering [68Ga]Ga-PSMA-11 and [18F]DCFPyL) compared to [99mTc]Tc-MDP BS in identifying bone metastases among prostate cancer patients. The quality of the selected studies was evaluated using the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) checklist.

Results

The meta-analysis included nine articles involving 702 patients. The sensitivity of PSMA PET/CT was higher compared to [99mTc]Tc-MDP BS (0.98 vs. 0.85, P < 0.01), while the specificity of PSMA PET/CT was also higher than [99mTc]Tc-MDP BS (0.97 vs. 0.70,P < 0.01). In subgroup analysis, the sensitivity of [68Ga]Ga-PSMA-11 PET/CT was higher compared to [99mTc]Tc-MDP BS (0.98 vs. 0.86), while the specificity of [68Ga]Ga-PSMA-11 PET/CT was also higher than [99mTc]Tc-MDP BS (0.98 vs. 0.65).

Conclusion

Our meta-analysis demonstrates that PSMA PET/CT exhibits superior sensitivity and specificity in comparison with [99mTc]Tc-MDP BS for identifying bone metastases in prostate cancer patients. Further research with head-to-head design is necessary to validate these results and evaluate the clinical effectiveness of these imaging methods.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier PROSPERO CRD42024545112.
Temporal artery biopsy in giant cell arteritis: clinical perspectives and histological patterns
Authors: Stamatis Pavlos , Turesson Carl , Mohammad Aladdin J.
Published: September 2024

Although its role has been debated, temporal artery biopsy (TAB) remains the gold standard for the diagnosis of cranial giant cell arteritis (GCA). The specificity of TAB is excellent and the sensitivity, albeit lower, is comparable with other diagnostic modalities used for the diagnosis of GCA. This outpatient procedure has a low rate of complications and is well integrated in the majority of healthcare systems. The length of the specimen, the number of the examined sections and the prolonged use of glucocorticoids before the biopsy may affect the outcome of the TAB as diagnostic tool. The typical histological findings in GCA are often characterized by granulomatous inflammation with infiltration of mononuclear cells with or without the presence of giant cell, varying degrees of external and internal elastic lamina damage and intimal thickening. Overlooking signs of inflammation in the adventitia and in connective tissue surrounding the temporal artery may lead to false negative results. The distinction between healed arteritis and age-related atherosclerosis may be challenging.
Methylated tirilazad may mitigate oligofructose-induced laminitis in horses
Authors: Tuniyazi Maimaiti , Tang Ruibo , Hu Xiaoyu , Zhang Naisheng
Published: September 2024

Laminitis is a serious health condition that can cause severe pain and lameness in horses. Due to lack of understanding of laminitis, treatments often fail to achieve the desired results. In recent years, we have begun to recognize that laminitis may involve a complex interaction between local and systemic inflammation. Dysbiosis of the gut microbiota has been linked in the development of systemic inflammation, and our previous findings suggest that the development of laminitis is closely linked to the production of harmful metabolites of the gut microbiota. In addition, it was found that localized lesions in the hoof, especially lamellar injuries, are the most direct cause of laminitis. Matrix metalloproteinases have been found to be strongly associated with the development of laminitis. Recent discovery has found that methylated tirilazad has a role in repairing laminar tissue in vitro. However, its efficacy in horses never has been studied. Therefore, we aimed to investigate the efficacy of methylated tirilazad (product name: PTP-102) in the prevention/treatment of oligofructose-induced laminitis. The results showed that oligofructose successfully induced laminitis in horses, resulting in detreated clinical signs. Blood indices (including inflammation-related indices and other related indices) were significantly increased. Observations of dissection and staining showed significant bleeding, swelling, and damage to hoof tissue. Analysis of the gut microbiota showed a significant decrease in abundance and diversity, and a significant increase in the relative abundance of specific bacteria. Following methylated tirilazad intervention, there were a significant improvement in clinical signs, blood markers and lamellar tissue damage. Additionally, methylated tirilazad positively influenced the gut microbiota structure by reducing the relative abundance of genera closely associated with the development of equine laminitis. This suggests that some of the therapeutic mechanism of methylated tirilazad may be linked to its effects on the gut microbiota. Notably, methylated tirilazad had better effect in the treatment group than the prophylactic group, indicating the post-diagnosis utility of methylated tirilazad for laminitis management.
Exploring diflunisal as a synergistic agent against Staphylococcus aureus biofilm formation
Authors: Salazar Maria , Shahbazi Nia Siavash , German Nadezhda A. , Awosile Babafela , Sabiu Saheed , Calle Alexandra
Published: September 2024

Staphylococcus aureus is a bacterial pathogen of considerable significance in public health, capable of inducing a diverse range of infectious diseases. One of the most notorious mechanisms used by S. aureus to survive and colonize the site of infection is its ability to form biofilms. Diflunisal, a non-steroidal anti-inflammatory drug (NSAID), is a known inhibitor of the Agr system in S. aureus, which is key in regulating biofilm formation. This study evaluated the effect of broad-spectrum antibiotics in combination with diflunisal on S. aureus biofilm density. Eight antibiotics were tested independently at different concentrations and in combination with diflunisal to assess their effect on S. aureus biofilm formation. When using the antibiotics alone and with diflunisal, a significant control effect on biofilm formation was observed (p < 0.05), irrespective of diflunisal presence, but did not achieve a complete biofilm growth inhibition. Over time, diflunisal influenced biofilm formation; however, such an effect was correlated with antibiotic concentration and exposure time. With amikacin treatments, biofilm density increased with extended exposure time. In the case of imipenem, doripenem, levofloxacin, and ciprofloxacin, lower doses and absence of diflunisal showed higher control over biofilm growth with longer exposure. However, in all cases, diflunisal did not significantly affect the treatment effect on biofilm formation. In the absence of antibiotics, diflunisal significantly reduced biofilm formation by 53.12% (p < 0.05). This study suggests that diflunisal could be a potential treatment to control S. aureus biofilms, but it does not enhance biofilm inhibition when combined with antibiotics.
The response of microbiome assembly within different niches across four stages to the cultivation of glyphosate-tolerant and conventional soybean varieties
Authors: Chao Shengqian , Sun Yu , Zhang Yin , Chen Yifan , Song Lili , Li Peng , Tang Xueming , Liang Jingang , Lv Beibei
Published: September 2024

Introduction

Plants are inherently connected with the microbiome, which plays a crucial role in regulating various host plant biological processes, including immunity, nutrient acquisition, and resistance against abiotic and biotic stresses. Many factors affect the interaction between plants and microbiome.

Methods and results

In this study, microbiome samples were collected from five niches (bulk soil, rhizoplane, root endosphere, phylloplane, and leaf endosphere) across four developmental stages (seedling, flowering, podding, and maturity) of various soybean varieties. Composition and structure of bacterial and fungal communities were analyzed using 16S rRNA gene and ITS (Internally Transcribed Spacer) region amplicon sequencing. It was observed that both niches and developmental stages significantly impact on the assembly and composition of soybean microbiome. However, variety, presence of a transgene, and glyphosate application had minimal effects on microbial communities. The dominant microbiome varied across the five niches, with most containing beneficial microbial communities capable of promoting plant growth or increasing disease resistance. Types and abundance of the dominant microbes affected network stability, potentially resulting in functional changes in different ecological niches.

Conclusion

This study provides theoretical evidence for microbial protection of plants against diseases and demonstrates that systematic analysis of the composition and diversity of soybean microbiomes can contribute to the development of biological control technologies.
Antimicrobial mechanisms and antifungal activity of compounds generated by banana rhizosphere Pseudomonas aeruginosa Gxun-2 against fusarium oxysporum f. sp. cubense
Authors: Lu Junming , Huang Yanbing , Liu Rui , Liang Ying , Zhang Hongyan , Shen Naikun , Yang Dengfeng , Jiang Mingguo
Published: September 2024

Introduction

Fusarium wilt of banana, also recognized as Panama disease, is caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense tropical race 4 (FOC TR4). In recent years, strategies utilizing biocontrol agents, comprising antifungal microorganisms and their associated bioactive compounds from various environments, have been implemented to control this destructive disease. Our previous study showed that Pseudomonas aeruginosa Gxun-2 had significant antifungal effects against FOC TR4. However, there has been little scientific investigation of the antibacterial or antifungal activity. The aim of this study was to isolate, identify and evaluate the inhibition strength of active compounds in P. aeruginosa Gxun-2, so as to explain the mechanism of the strain inhibition on FOC TR4 from the perspective of compounds.

Methods

The main antibacterial compounds of strain Gxun-2 were isolated, purified and identified using by fermentation extraction, silica gel column chromatography, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) techniques. The effect of the compounds on the mycelial growth, morphology and spore germination of strain FOC TR4 was observed by 96-well plate method and AGAR diffusion method.

Results

Among the metabolites produced by the strain, four antifungal compounds which were identified phenazine (C12H8N2), phenazine-1-carboxylic acid (PCA) (C13H8N2O2), 2-acetamidophenol (C8H9NO2) and aeruginaldehyde (C10H7NO2S) were identified through HPLC and NMR. Of these compounds, phenazine and PCA exhibited the most pronounced inhibitory effects on the spore germination and mycelial growth of FOC TR4. Phenazine demonstrated potent antifungal activity against FOC TR4 with a minimum inhibitory concentration (MIC) of 6.25 mg/L. The half-maximal effective concentration (EC50) was calculated to be 26.24 mg/L using the toxicity regression equation. PCA exhibited antifungal activity against FOC TR4 with an MIC of 25 mg/L and an EC50 of 89.63 mg/L. Furthermore, phenazine and PCA triggered substantial morphological transformations in the mycelia of FOC TR4, encompassing folding, bending, fracturing, and diminished spore formation.

Discussion

These findings indicate that strain Gxun-2 plays a crucial role in controlling FOC TR4 pathogenesis, predominantly through producing the antifungal compounds phenazine and PCA, and possesses potential as a cost-efficient and sustainable biocontrol agent against Fusarium wilt of banana in forthcoming times.