bluuebunny/crossref_metadata_2025 huggingface dataset:
Coping with COVID: Use, factor structure, and health behavior implications of specific emotion regulation strategies during the coronavirus pandemic
Authors: Langley Erika B. , Vornlocher Carley , Manapat Patrick D. , Shiota Michelle N.
Published: July 2023

This series of studies examined U.S. individuals' use of specific emotion regulation/coping strategies during the early months of the COVID‐19 pandemic, investigated the factor structure among strategies during this universally experienced stressor, and the extent to which these factors predicted engagement in COVID‐related health‐promoting behaviors. In Study 1, participants (N = 520) rated their use of 17 strategies for coping with pandemic‐related stress during the past 24 h. Differences emerged in strategy use across demographic groups (age, race, income). Results of exploratory factor analysis suggest a factor structure grouping strategies in terms of goals beyond emotion regulation per se, rather than phases of the emotion process or a binary adaptive versus maladaptive distinction. In Study 2 (N = 264), participants reported daily on their coping strategy use and weekly on their engagement in COVID‐specific health behaviors for 22 days. Results of confirmatory factor analysis replicate the factor structure found in Study 1. Some significant associations of coping strategy use with health‐promoting behaviors were observed, but these were sporadic and largely involved baseline measures rather than predicting change over time. Theoretical and practical implications are discussed.
Unusual transformation of Leishmania spp. amastigotes to promastigotes in a bone marrow sample from a Greyhound dog
Authors: Rodríguez‐Cariño Carolina , López Loarte Javier , Marí‐Martorell Daniel , Pantchev Nikola , Hernandez Martinez Leticia
Published: July 2023

The objective of the study was to confirm the presence of different morphological forms of Leishmania infantum promastigotes on bone marrow aspirates from a Spanish Greyhound with canine leishmaniosis.
Understanding the reactive interfacial flow dynamics with production of viscoelastic material through large amplitude oscillatory shear (LAOS) measurements of the viscoelastic interface
Authors: Yagi Harumi , Nagatsu Yuichiro , Takano Masayoshi , Suzuki Ryuta X.
Published: July 2023

In this study, the interfacial flow dynamics involving a chemical reaction that produces viscoelastic material at the interface between two liquids is experimentally investigated, and the material is evaluated using interfacial large amplitude oscillatory shear (LAOS) measurements. The flow dynamics indicates fingering patterns at low injection flow rates and fracturing patterns at high flow rates in Hele-Shaw cells, where a more viscous xanthan gum solution is displaced by the less viscous Fe(NO3)3 solution with various concentrations of Fe(NO3)3. The threshold flow rate value of such a transition is different for various concentrations of Fe(NO3)3. Although such a transition without chemical reactions has been discussed, the factors responsible for the transition remain unclear. The flow dynamics in Hele-Shaw cells is considered to flow under large deformation, which exceeds the small amplitude oscillatory shear condition but is under the LAOS condition. Therefore, LAOS measurement of the viscoelastic interface is performed for various concentrations of Fe(NO3)3. Using the characteristic properties extracted from the LAOS measurements, the elastic and viscous forces of the viscoelastic interface are evaluated. We show the transition from fingering to fracturing patterns when the elastic force exceeds a certain value. These findings highlight that rheology under large deformation of the viscoelastic interface plays a crucial role in interfacial flow, where viscoelastic materials are produced by chemical reactions at the interface. In addition, this study should be an example of the successful elucidation of physical phenomena by interfacial LAOS, which has been reported in a very limited number of studies.
Effect of a 24-Week Concurrent Exercise Intervention on Neck Adiposity and Its Distribution in Young Adults: The ACTIBATE Randomized Controlled Trial
Authors: Arias-Tellez Maria Jose , Acosta Francisco M. , Merchan-Ramirez Elisa , Martínez-Téllez Borja , Delgado Guillermo-Sánchez , Llamas-Elvira Jose M. , Ruiz Jonatan R.
Published: July 2023

Neck adipose tissue (NAT) accumulation and neck circumference are independent predictors of cardiometabolic risk (CMR) and low-grade chronic inflammation in young adults. The present study examines whether a 24-week concurrent exercise intervention can reduce NAT volume and neck circumference in young adults, and whether any changes in these variables are related to changes in body composition, CMR, and the inflammatory profile. Seventy-four participants (51 women, age 22 ± 2 years) were included in the main analyses, after being randomly assigned to either a (a) control (n = 34), (b) moderate-intensity exercise (n = 19), or (c) vigorous-intensity exercise (n = 21) group. Participants in the exercise groups trained 3–4 days/week (endurance + resistance exercise training). NAT volume and NAT distribution across different depots were estimated using computed tomography before and after the intervention. Anthropometric variables, body composition (determined by dual-energy X-ray absorptiometry), and CMR/inflammatory markers were also recorded. The exercise intervention did not reduce the total NAT volume, nor was NAT distribution affected (p > .05). However, it did reduce neck circumference in the vigorous-intensity exercise group compared with the moderate-intensity exercise and control groups (by 0.8 and 1 cm, respectively, p ≤ .05). Changes in total NAT and neck circumference were positively, albeit weakly, related (adj. R2: .05–.21, all p ≤ .05) to changes in body weight and adiposity, leptin (only total NAT), and CMR (only neck circumference). Altogether 24 weeks of concurrent exercise does not appear to reduce NAT accumulation in young adults, but may slightly reduce neck circumference in those who partake in vigorous exercise.
New Zealand Blackcurrant Increases Postexercise Hypotension Following Sustained Moderate-Intensity Exercise
Authors: Shan Yusen , Cook Matthew David
Published: July 2023

Previous observations demonstrate New Zealand blackcurrant (NZBC) extract to alter cardiovascular responses at rest without prior exercise. However, the prolonged effects of NZBC on blood pressure and heart rate variability following exercise are not known. Participants (n15 [five women], age: 31 ± 9 years, maximal oxygen uptake: 44 ± 9 ml·kg−1·min−1) undertook a control condition of 2 hr of lying supine rest. Subsequently, in a double-blind, placebo (PLA)-controlled, randomized crossover design participants completed 1 hr of treadmill exercise at 50% maximal oxygen uptake followed by 2-hr supine rest with blood pressure and heart rate variability measurement following a 7-day intake of NZBC and PLA. With NZBC, there was an increase in average fat oxidation (NZBC: 0.24 ± 0.11 vs. PLA: 0.17 ± 0.11 g/min, p = .005), and larger high-frequency relative power during the exercise (p = .037). In the 2-hr rest period, delta change for systolic pressure was larger with NZBC than PLA (Control vs. NZBC: −5.6 ± 6.4, Control vs. PLA: −3.5 ± 6.0 mmHg, p = .033) but was not different for diastolic or mean arterial pressure. There were no alterations in heart rate variabilities during the 2 hr following the exercise with NZBC. A 7-day intake of NZBC causes a larger postexercise hypotension response in young, physically active men and women following 1 hr of treadmill exercise at 50% maximal oxygen uptake.
Metabolic interaction models recapitulate leaf microbiota ecology
Authors: Schäfer Martin , Pacheco Alan R. , Künzler Rahel , Bortfeld-Miller Miriam , Field Christopher M. , Vayena Evangelia , Hatzimanikatis Vassily , Vorholt Julia A.
Published: July 2023

Resource allocation affects the structure of microbiomes, including those associated with living hosts. Understanding the degree to which this dependency determines interspecies interactions may advance efforts to control host-microbiome relationships. We combined synthetic community experiments with computational models to predict interaction outcomes between plant-associated bacteria. We mapped the metabolic capabilities of 224 leaf isolates from
Arabidopsis thaliana
by assessing the growth of each strain on 45 environmentally relevant carbon sources in vitro. We used these data to build curated genome-scale metabolic models for all strains, which we combined to simulate >17,500 interactions. The models recapitulated outcomes observed in planta with >89% accuracy, highlighting the role of carbon utilization and the contributions of niche partitioning and cross-feeding in the assembly of leaf microbiomes.
Principles of human pre-60
S
biogenesis
Authors: Vanden Broeck Arnaud , Klinge Sebastian
Published: July 2023

During the early stages of human large ribosomal subunit (60
S
) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60
S
particles by an unknown mechanism. Here, we report a series of cryo–electron microscopy structures of human nucleolar and nuclear pre-60
S
assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre–ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60
S
particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.
The sound of silence
Authors: Clasen Sara
Published: July 2023

How flagellins from commensals evade innate immunity
Museum ethics—more than lip service
Authors: Thorp H. Holden
Published: July 2023

In the past few years, museums around the world have started to grapple with questions about the origins and ethics of their collections. This includes the acquisition and maintenance of natural history specimens. As museums examine their missions and processes, it seemed like a good time to talk to Sean Decatur, the new president of the American Museum of Natural History (AMNH) in New York City. He spoke with me (see the full conversation here) about the museum’s research and how collaborations between museums and partner nations should aspire to assemble collections that responsibly disseminate information about human cultures, the natural world, and the Universe.
After race ban, other routes to diversity beckon
Authors: Mervis Jeffrey
Published: July 2023

Equity advocates see paths to increasing minority representation—but say it won’t be easy
Phytoplankton Producer Species and Transformation of Released Compounds over Time Define Bacterial Communities following Phytoplankton Dissolved Organic Matter Pulses
Authors: Eigemann Falk , Rahav Eyal , Grossart Hans-Peter , Aharonovich Dikla , Voss Maren , Sher Daniel
Published: July 2023

Phytoplankton-bacterium interactions influence biogeochemical cycles of global importance. Phytoplankton photosynthetically fix carbon dioxide and subsequently release the synthesized compounds as dissolved organic matter (DOMp), which becomes processed and recycled by heterotrophic bacteria.
Metagenomic Profiles of Yak and Cattle Manure Resistomes in Different Feeding Patterns before and after Composting
Authors: Fan Qingshan , Zhang Jing , Shi Hairen , Chang Shenghua , Hou Fujiang
Published: July 2023

The recent increase in the prevalence of antibiotic resistance genes (ARGs), biocide resistance genes (BRGs), and metal resistance genes (MRGs) in livestock manure poses risks to human health. Composting is known to be a promising technology for reducing the abundance of resistance genes.
Serine peptidases and increased amounts of soluble proteins contribute to heat priming of the plant pathogenic fungus
Botrytis cinerea
Authors: Zhang Mingzhe , Trushina Naomi Kagan , Lang Tabea , Hahn Matthias , Pasmanik-Chor Metsada , Sharon Amir
Published: July 2023

Botrytis cinerea
causes gray mold disease in leading crop plants. The disease develops only at cool temperatures, but the fungus remains viable in warm climates and can survive periods of extreme heat. We discovered a strong heat priming effect in which the exposure of
B. cinerea
to moderately high temperatures greatly improves its ability to cope with subsequent, potentially lethal temperature conditions. We showed that priming promotes protein solubility during heat stress and discovered a group of priming-induced serine-type peptidases. Several lines of evidence, including transcriptomics, proteomics, pharmacology, and mutagenesis data, link these peptidases to the
B. cinerea
priming response, highlighting their important roles in regulating priming-mediated heat adaptation. By imposing a series of sub-lethal temperature pulses that subverted the priming effect, we managed to eliminate the fungus and prevent disease development, demonstrating the potential for developing temperature-based plant protection methods by targeting the fungal heat priming response.

IMPORTANCE

Priming is a general and important stress adaptation mechanism. Our work highlights the importance of priming in fungal heat adaptation, reveals novel regulators and aspects of heat adaptation mechanisms, and demonstrates the potential of affecting microorganisms, including pathogens through manipulations of the heat adaptation response.
Disc Test for Detecting Staphylococcus aureus Strains Producing Type A and Type C β-Lactamases
Authors: George C. R. Robert , Lahra Monica M. , Nguyen Thanh , Gatus Barrie
Published: July 2023

The key significance of this article is that it details a straightforward method of performing a disc test that can differentiate
Staphylococcus aureus
isolates that are likely to be associated with a cefazolin inoculum effect and theoretical risk of cefazolin treatment failure from isolates that are less likely to be associated with a cefazolin inoculum effect.
Acinetobacter baumannii GC2 Sublineage Carrying the
aac
(
6
′)-
Im
Amikacin, Netilmicin, and Tobramycin Resistance Gene Cassette
Authors: Harmer Christopher J. , Nigro Steven J. , Hall Ruth M.
Published: July 2023

Aminoglycosides are important therapeutic options for treatment of
Acinetobacter
infections. Here, we show that a little-known aminoglycoside resistance gene,
aac(6′)-Im
(
aacA16
), that confers amikacin, netilmicin, and tobramycin resistance has been circulating undetected for many years in a sublineage of
A. baumannii
global clone 2 (GC2), generally with a second aminoglycoside resistance gene,
aacC1
, which confers resistance to gentamicin.
Initiation of Mesoarchean nascent subduction zone in the North China Craton
Authors: Yao Jiachen , Wang Wei , Gao Lei , Hu Fangyang , Lu Denggang , He Xin , Guo Lishuang
Published: July 2023

Exploring the nature of Archean plate tectonics is crucial for understanding the geodynamic evolution of early Earth. While previous studies have focused on (ultra-)mafic metavolcanic and tonalite−trondhjemite−granodiorite (TTG) rocks, the crust-mantle interactions of Archean intermediate magmatism have received much less attention. Mesoarchean (ca. 2936−2855 Ma) dioritic and tonalitic-trondhjemitic magmatism occurred in the Jiaobei terrane, North China Craton. While low-silica (65.67−68.82 wt%) TTG magmatism persisted throughout the entire magmatic period, both the high-silica (69.79−74.54 wt%) TTG and biotite dioritic (MgO of 1.52−2.45 wt%) magmatism were transient (ca. 2922−2901 Ma). They were mainly sourced from metabasaltic crust, though the biotite dioritic and low-silica TTG rocks show variable mantle input. In comparison, the hornblende dioritic magmatism (MgO of 2.07−5.00 wt%) occurred later (ca. 2912−2865 Ma) and was derived from melt-metasomatized mantle sources. Spatially, the magmatism became younger from northeast to southwest. Combined with increasing crustal melting pressures after ca. 2920 Ma and mildly elevated zircon δ18O (up to +6.8‰) of TTG rocks, a Mesoarchean nascent subduction zone is proposed for the Jiaobei terrane that is featured by the recycling of supracrustal basaltic rocks to thickened lower crust (forming high-pressure and high-δ18O TTG) and subsequent melt-related mantle metasomatism (forming relatively high-Mg hornblende dioritic rocks). The recycled supracrustal materials may have lubricated the basal part of the thickened crust and then triggered the opening of a nascent mantle wedge. Further compilation of data from global Archean dioritic and TTG rocks reveals chemical discontinuities at ca. 3.6−3.5 Ga and after ca. 3.0 Ga; these periods are marked by the occurrence of both high-Mg (median MgO >3 wt%) dioritic magmatism and high-(La/Yb)N (median >30) and high-δ18O (median >5.9‰) TTG magmatism. These data, along with independent geological evidence, suggest that the nascent subduction processes may have initiated locally at ca. 3.6−3.5 Ga but globally after ca. 3.0 Ga.
Early Neoproterozoic tectonics in the Marwar Crustal Block, NW India, the relevance of the Phulad Shear Zone, and implications for Rodinia reconstruction
Authors: Chatterjee Sadhana M. , Roy Alip , Manna Anirban , Sarkar Ayan Kumar
Published: July 2023

Palaeomagnetic studies in the Malani Igneous Suite (ca. 770–750 Ma) of the Marwar Crustal Block, NW India, provide essential constraints on palaeogeographic reconstructions of the Rodinia Supercontinent. The Malani Igneous Suite is intrusive into megacrystic granite and granitegneissic enclaves of the Marwar Crustal Block. A crustalscale NE–SW ductile transpressional shear zone with a southeasterly dip known as the Phulad Shear Zone (820–810 Ma) separates this Marwar Crustal Block from the rocks farther east. The preshearing tectonic evolution of the Marwar Crustal Block is poorly understood.

Three phases of ductile deformations (D1, D2, and D3, with associated fabrics S1, S2, and S3) were identified in the Marwar Crustal Block. The D1 deformation is restricted to enclave gneisses. The megacrystic granite was emplaced syntectonically during D2 deformation. The S2 magmatic foliation (NNW–SSE) in the megacrystic granite is oblique to the Phulad Shear Zone. The D3 deformation in the megacrystic granite is synchronous with the Phulad Shear Zone mylonites. Another porphyritic granite (Phulad granite, ca. 820 Ma) emplaced syntectonically during D3 deformation along and across the Phulad Shear Zone. U-Pb zircon dates in the Marwar Crustal Block document ca. 890 Ma and ca. 860 Ma magmatic dates. U-Pb-Th monazite dates in the Marwar Crustal Block show a strong peak at ca. 820 Ma. By integrating critical field relations, deformational patterns, and geochronology, we argue that the Marwar Crustal Block shows distinct geological evolution prior to its accretion with the remaining parts of India. We propose that the accretion of the Marwar Crustal Block must be younger than ca. 860 Ma and culminated during ca. 820–810 Ma to form the Greater India landmass along the Phulad Shear Zone.
Enhancement of the hardness and impact strength of epoxy-based metal oxide nanocomposites through hybridization
Authors: Mustafa Bakhan S. , Jamal Gelas M. , Abdullah Omed Gh.
Published: July 2023

In this study, hybrid polymer nanocomposites were produced by combining epoxy (EP) with varying amounts of zirconia (ZrO[Formula: see text] and yttria (Y2O[Formula: see text] nanoparticles (NPs), reinforced with multi-walled carbon nanotube (MWCNT) and glass fiber (GF). The objective of this study is to develop and examine the performance of EP nanocomposites through hybridization; for this purpose, hardness and impact tests were conducted in accordance with American Society for Testing and Materials (ASTM) standards. Different sets were produced to investigate the effect of used fillers, including EP/ZrO2, EP/Y2O3, EP/MWCNT, EP/GF, EP/ZrO2/Y2O3, EP/MWCNT/ZrO2/Y2O3, and EP/MWCNT/GF/ZrO2/Y2O3. All findings were compared with the net EP sample. The results showed that the EP nanocomposite samples with low NPs content exhibit much higher mechanical performance than net EP. Furthermore, the additions of MWCNT and GF separately at 0.1 and 0.06[Formula: see text]wt.% have increased the impact strength, respectively, to 165.2% and 338.8%, compared with pure EP. As a result, the presence of MWCNTs and GF in the EP-based nanocomposite matrix could make the product both stronger and tougher.
Prediction of a Positive ANA Result for a Rheumatological Diagnosis in an Outpatient Setting
Authors: Chu Chiu Sum , Ho Ling Yin , Mok Chi Chiu
Published: July 2023

Objective: To study the predictive value of a positive anti-nuclear antibody (ANA) for a rheumatological diagnosis in an outpatient setting.

Methods: Individuals who were referred to the rheumatology outpatient clinics because of a positive ANA between July 2014 and June 2015 were retrospectively reviewed. Presenting symptoms in addition to a positive ANA and whether a final rheumatological diagnosis was made were recorded. The positive predictive value of a positive ANA and its titer for a rheumatological diagnosis, with and without accompanying symptoms was evaluated.

Results: A total of 230 patients were included (82% women, age 47.7 ± 14.1 years [range 18-84]). Family medicine and the general outpatient clinic were the main sources of referral (32.2%), followed by ophthalmology (13.0%) and otorhinolaryngology (11.7%). A final rheumatological diagnosis was made in 54 (23.5%) patients, with rheumatoid arthritis being the commonest diagnosis (40.7%). In the absence of any associated symptoms, the predictive value of a positive ANA was 0%. The presence of Raynaud’s phenomenon (100%), joint swelling (59.5%), and joint stiffness (48.9%) predicted a better final rheumatological diagnosis along with a positive ANA. ANA titers of 1:80 or less had a low sensitivity for rheumatic diseases. A receiver operating characteristic (ROC) curve analysis showed that an ANA titer of [Formula: see text]1:128 best predicted a rheumatological diagnosis (AUC 0.78 [0.71–0.85]; sensitivity 0.78; specificity 0.64).

Conclusions: To improve the prediction for a rheumatological diagnosis, referral for a positive ANA test should be more appropriately done with compatible symptoms.
WVC: Towards Secure Device Paring for Mobile Augmented Reality
Authors: Zhang Qian , Yang Zheng , Li Fan , Zhu Biaokai , Chen Pengpeng
Published: July 2023

In mobile augmented reality applications, how to build a secure device connection between two previously unassociated devices without prior set-up is challenging, which also refers to the problem of device pairing. Most existing approaches to device pairing either have certain limits to be utilized in the environment of augmented reality or lack considerations of security issues. In this article, we design WVC, an intuitive, user-friendly, and secure device paring system for mobile augmented reality. It enables users to connect to a neighboring device by waving a finger to click towards it in the air. The system uses critical features from finger tracking trajectories to understand which device a user wants to interact with. Then, it designs key generation and correction algorithms to enhance security in device communication. In addition, we present solutions to defend against malicious attacks. We implement and test the system with 10 volunteers. The experimental results demonstrate the feasibility and effectiveness of the system under varied scenarios in which devices are close to each other at a small angle or located at different heights within 2
m
.
Tramadol is a performance-enhancing drug in highly trained cyclists: a randomized controlled trial
Authors: Mauger Alexis R. , Thomas Trudy , Smith Samuel A. , Fennell Christopher R. J.
Published: July 2023

In the current study, when cycling with tramadol participants completed a time trial on average 50 s faster and at a 9 W higher power output than the placebo control. The study used both a fixed intensity and self-paced time trial exercise tasks to reflect the demands of a stage race. The outcomes from this study were used by the World Anti-Doping Agency to inform their addition of tramadol to the Prohibited List in 2024.
The forearm vascular response to sympathetic activation is attenuated in female, but not male, participants following acute intermittent hypoxia
Authors: Jacob Dain W. , Morgenthaler Leandra D. , Harper Jennifer L. , Limberg Jacqueline K.
Published: July 2023

We examined the effects of acute intermittent hypoxia (AIH) on the vascular response to sympathetic activation and acute hypoxia. Present findings show, despite no effect of AIH on the vascular response to hypoxia, the forearm vasoconstrictor response to acute sympathetic activation is attenuated in females following AIH, independent of estradiol state. These data provide mechanistic understanding of potential benefits of AIH, as well as the impact of biological sex.
Use of Doxycycline in a Patient following Minocycline-Induced Lupus
Authors: Newman Katherine Quinn , Castles Charles Guy
Published: July 2023

Minocycline, a tetracycline antibiotic, is commonly used to treat rosacea and acne vulgaris. A rare adverse reaction of minocycline use is the development of drug-induced lupus. Fortunately, most patients recover from minocycline-induced lupus (MIL) after the drug is discontinued. However, many patients, after recovering from MIL, may desire further treatment for their acne and may consider doxycycline, a close relative of minocycline. Though no cases of doxycycline-induced lupus have been reported, there is little guidance in the medical literature as to whether doxycycline poses a particular risk to patients who have recovered from MIL. We report the long-term follow-up of a patient who recovered from MIL (the diagnosis satisfying clinical and laboratory criteria) and was treated for 8 years with various forms of doxycycline without any untoward effects, suggesting that, at least in some cases, doxycycline can be used safely following MIL.
A Rare Case of Blastic Plasmacytoid Dendritic Cell Neoplasm Occurred in Postchemotherapy of Breast Cancer
Authors: Tong Jiankun , Aksenov Sergei , Siegel Beth M. , Wei Lihong , Rodgers William H.
Published: July 2023

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and highly aggressive hematologic malignancy that arises from plasmacytoid dendritic cells. BPDCN typically presents with skin lesions and may involve peripheral blood, bone marrow, lymph nodes, or extranodal sites. It usually arises de novo, and some BPDCN cases are associated with or develop into myeloid neoplasms. Here, we report a case of a 57-year-old female presenting with cervical lymphadenopathy and skin rashes during the COVID-19 pandemic in 2021 following multiple types of postmastectomy therapy for breast cancer. The patient was ultimately diagnosed with BPCDN by lymph node biopsy. To the best of our knowledge, this is the first case report of BPDCN occurring postchemotherapy of breast cancer.
Data from Cost-effectiveness of p16/Ki-67 Dual-Stained Cytology Reflex Following Co-testing with hrHPV Genotyping for Cervical Cancer Screening
Authors: Harper Diane M. , Anderson Rye J. , Baker Ed , Yu Tiffany M.
Published: July 2023

Abstract
The first biomarker-based cervical cancer screening test, p16/Ki-67 dual-stained cytology (DS), has been clinically validated and approved in the United States for triage of women being screened for cervical cancer who test positive for high-risk human papillomavirus (hrHPV). The primary aim of this work is to evaluate the cost-effectiveness of DS triage after co-testing findings of positive non-16/18 HPV types and atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesions cytology. A payer-perspective Markov microsimulation model was developed to assess the impact of DS reflex testing. Each comparison simulated 12,250 screening-eligible women through health states defined by hrHPV status and genotype, cervical intraepithelial neoplasia grades 1–3, invasive cervical cancer (ICC) by stage, and cancer-related or non-cancer death. Screening test performance data were from the IMPACT clinical validation trial. Transition probabilities were from population and natural history studies. Costs of baseline medical care, screening visits, tests, procedures, and ICC were included. DS reflex after co-testing was cost-effective with incremental cost-effectiveness ratios per quality-adjusted life-year gained of $15,231 [95% confidence interval (CI), $10,717–$25,400] compared with co-testing with hrHPV pooled primary and genotyped reflex testing, and $23,487 (95% CI, $15,745–$46,175) compared with co-testing with hrHPV genotyping with no reflex test. Screening and medical costs and life-years increased, while ICC costs and risk of ICC death decreased. Incorporating DS reflex into co-testing cervical cancer screening algorithms is projected to be cost-effective.
Prevention Relevance:
The p16/Ki-67 dual-stained cytology (DS) test was recently approved in the United States as a reflex test for cervical cancer screening following positive high-risk human papillomavirus (hrHPV) test results. Adding DS reflex to hrHPV and cervical cytology co-testing strategies in the United States is expected to be cost-effective per life-year or quality-adjusted life-year gained.
Table S1 from Proteogenomic approaches for the identification of NF1/neurofibromin-depleted estrogen receptor positive breast cancers for targeted treatment
Authors: Kim Beom-Jun , Zheng Ze-Yi , Lei Jonathan T. , Holt Matthew V. , Chen Anran , Peng Jianheng , Fandino Diana , Singh Purba , Kennedy Hilda , Dou Yongchao , Chica-Parrado María del Rosario , Bikorimana Emmanuel , Ye Dan , Wang Yunguan , Hanker Ariella B. , Mohamed Nada , Hilsenbeck Susan G. , Lim Bora , Asirvatham Jaya Ruth , Sreekumar Arun , Zhang Bing , Miles George , Anurag Meenakshi , Ellis Matthew J. , Chang Eric C
Published: July 2023

Table S1. Correlation between levels of NF1 and protein kinases in the PDXs.
Figure S2 from Proteogenomic approaches for the identification of NF1/neurofibromin-depleted estrogen receptor positive breast cancers for targeted treatment
Authors: Kim Beom-Jun , Zheng Ze-Yi , Lei Jonathan T. , Holt Matthew V. , Chen Anran , Peng Jianheng , Fandino Diana , Singh Purba , Kennedy Hilda , Dou Yongchao , Chica-Parrado María del Rosario , Bikorimana Emmanuel , Ye Dan , Wang Yunguan , Hanker Ariella B. , Mohamed Nada , Hilsenbeck Susan G. , Lim Bora , Asirvatham Jaya Ruth , Sreekumar Arun , Zhang Bing , Miles George , Anurag Meenakshi , Ellis Matthew J. , Chang Eric C
Published: July 2023

Figure S2. Binimetinib and fulvestrant inhibited the intended targets in the two PDXs that
did not regress after treatment.
Supplementary Figure 8 from Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma
Authors: Nisnboym Michal , Vincze Sarah R. , Xiong Zujian , Sneiderman Chaim T. , Raphael Rebecca A. , Li Bo , Jaswal Ambika P. , Sever ReidAnn E. , Day Kathryn E. , LaToche Joseph D. , Foley Lesley M. , Karimi Hanieh , Hitchens T. Kevin , Agnihotri Sameer , Hu Baoli , Rajasundaram Dhivyaa , Anderson Carolyn J. , Blumenthal Deborah T. , Pearce Thomas M. , Uttam Shikhar , Nedrow Jessie R. , Panigrahy Ashok , Pollack Ian F. , Lieberman Frank S. , Drappatz Jan , Raphael Itay , Edwards Wilson B. , Kohanbash Gary
Published: July 2023

MRI analysis of tumor volume prior to immuno-PET.
Supplementary Figure 3 from Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma
Authors: Nisnboym Michal , Vincze Sarah R. , Xiong Zujian , Sneiderman Chaim T. , Raphael Rebecca A. , Li Bo , Jaswal Ambika P. , Sever ReidAnn E. , Day Kathryn E. , LaToche Joseph D. , Foley Lesley M. , Karimi Hanieh , Hitchens T. Kevin , Agnihotri Sameer , Hu Baoli , Rajasundaram Dhivyaa , Anderson Carolyn J. , Blumenthal Deborah T. , Pearce Thomas M. , Uttam Shikhar , Nedrow Jessie R. , Panigrahy Ashok , Pollack Ian F. , Lieberman Frank S. , Drappatz Jan , Raphael Itay , Edwards Wilson B. , Kohanbash Gary
Published: July 2023

CD69 analysis in single-cell RNA-seq data.
FIGURE 4 from Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma
Authors: Nisnboym Michal , Vincze Sarah R. , Xiong Zujian , Sneiderman Chaim T. , Raphael Rebecca A. , Li Bo , Jaswal Ambika P. , Sever ReidAnn E. , Day Kathryn E. , LaToche Joseph D. , Foley Lesley M. , Karimi Hanieh , Hitchens T. Kevin , Agnihotri Sameer , Hu Baoli , Rajasundaram Dhivyaa , Anderson Carolyn J. , Blumenthal Deborah T. , Pearce Thomas M. , Uttam Shikhar , Nedrow Jessie R. , Panigrahy Ashok , Pollack Ian F. , Lieberman Frank S. , Drappatz Jan , Raphael Itay , Edwards Wilson B. , Kohanbash Gary
Published: July 2023

Immuno-PET of 89Zr-DFO-CD69 Ab visualizes the TME after ICI treatment in a GBM mouse model. Mice were inoculated with GL261 cells, and evaluated by immuno-PET of 89Zr-DFO-CD69 Ab, comparing the treatment (ICI) group to the control group (represented by orange and blue dots, respectively). A, Schematic showing timeline of tumor inoculation, ICI treatment, tail vein injection of 89Zr-DFO-CD69 Ab, immuno-PET, and BioD. B, Representative coronal head images from immuno-PET of 89Zr-DFO-CD69 Ab of ICI-treated and control mice at designated time points. Scales show SUVs of PET (SUV; colored) and CT (HU; gray). C and D, Comparison between ICI-treated mice and control at designated time points of tumor-specific regions’ SUVmax (C) and SUVmax TBR (D). E, Representative coronal-ventral and sagittal 3D whole-body MIP PET images of 89Zr-DFO-CD69 Ab of control and ICI-treated mice acquired 6 days after tracer administration. The scale shows SUVs of PET. F, Day 6 BioD results of blood- and tumor site–associated radioactivity, assessed as %ID/g. In C, D, and F, bars show means ± SEM. Scattered dots represent individual mice. Shown are representative data of three independent experiments, n = 5 per group. C and D, Multiple unpaired t test with Welch correction. F, Two-way ANOVA with multiple comparison test (*, P < 0.05; **, P <0.01; ***, P <0.001). IP, intraperitoneal.
Matrix-Binding, N-Cadherin–Targeting Chimeric Peptide Inhibits Intimal Thickening but Not Endothelial Repair in Balloon-Injured Carotid Arteries
Authors: Burke-Kleinman Jonah , Rubianto Jonathan , Hou Guangpei , Santerre J. Paul , Bendeck Michelle P.
Published: July 2023

BACKGROUND:

Treatment of occluded vessels can involve angioplasty, stenting, and bypass grafting, which can be limited by restenosis and thrombosis. Drug-eluting stents attenuate restenosis, but the current drugs used are cytotoxic, causing smooth muscle cell (SMC) and endothelial cell (EC) death that may lead to late thrombosis. N-cadherin is a junctional protein expressed by SMCs, which promotes directional SMC migration contributing to restenosis. We propose that engaging N-cadherin with mimetic peptides can act as a cell type–selective therapeutic strategy to inhibit polarization and directional migration of SMCs without negatively impacting ECs.

METHODS:

We designed a novel N-cadherin–targeting chimeric peptide with a histidine-alanine-valine cadherin-binding motif, combined with a fibronectin-binding motif from
Staphylococcus aureus
. This peptide was tested in SMC and EC culture assays of migration, viability, and apoptosis. Rat carotid arteries were balloon injured and treated with the N-cadherin peptide.

RESULTS:

Treating scratch-wounded SMCs with the N-cadherin–targeting peptide inhibited migration and reduced polarization of wound-edge cells. The peptide colocalized with fibronectin. Importantly, EC junction, permeability, or migration was not impacted by peptide treatment in vitro. We also demonstrated that the chimeric peptide persisted for 24 hours after transient delivery in the balloon-injured rat carotid artery. Treatment with the N-cadherin–targeting chimeric peptide reduced intimal thickening in balloon-injured rat carotid arteries at 1 and 2 weeks after injury. Reendothelialization of injured vessels after 2 weeks was unimpaired by peptide treatment.

CONCLUSIONS:

These studies show that an N-cadherin–binding and fibronectin-binding chimeric peptide is effective in inhibiting SMC migration in vitro and in vivo and limiting neointimal hyperplasia after balloon angioplasty without affecting EC repair. These results establish the potential of an advantageous SMC-selective strategy for antirestenosis therapy.
Long-Term Prevalence of Systolic Dysfunction in MYBPC3 Versus MYH7-Related Hypertrophic Cardiomyopathy
Authors: Beltrami Matteo , Fedele Elisa , Fumagalli Carlo , Mazzarotto Francesco , Girolami Francesca , Ferrantini Cecilia , Coppini Raffaele , Tofani Lorenzo , Bertaccini Bruno , Poggesi Corrado , Olivotto Iacopo
Published: July 2023

BACKGROUND:

The 2 sarcomere genes most commonly associated with hypertrophic cardiomyopathy (HCM), MYBPC3 (myosin-binding protein C3) and MYH7 (β-myosin heavy chain), are indistinguishable at presentation, and genotype-phenotype correlations have been elusive. Based on molecular and pathophysiological differences, however, it is plausible to hypothesize a different behavior in myocardial performance, impacting lifetime changes in left ventricular (LV) function.

METHODS:

We reviewed the initial and final echocardiograms of 402 consecutive HCM patients with pathogenic or likely pathogenic MYBPC3 (n=251) or MYH7 (n=151) mutations, followed over 9±8 years.

RESULTS:

At presentation, MYBPC3 patients were less frequently obstructive (15% versus 26%;
P
=0.005) and had lower LV ejection fraction compared with MYH7 (66±8% versus 68±8%, respectively;
P
=0.03). Both HCM patients harboring MYBPC3 and MYH7 mutations exhibited a small but significant decline in LV systolic function during follow-up; however, new onset of severe LV systolic dysfunction (LV ejection fraction, <50%) was greater among MYBPC3 patients (15% versus 5% among MYH7;
P
=0.013). Prevalence of grade II/III diastolic dysfunction at final evaluation was comparable between MYBPC3 and MYH7 patients (
P
=0.509). In a Cox multivariable analysis, MYBPC3-positive status (hazard ratio, 2.53 [95% CI, 1.09–5.82];
P
=0.029), age (hazard ratio, 1.03 [95% CI, 1.00–1.06];
P
=0.027), and atrial fibrillation (hazard ratio, 2.39 [95% CI, 1.14–5.05];
P
=0.020) were independent predictors of severe systolic dysfunction. No statistically significant differences occurred with regard to incidence of atrial fibrillation, heart failure, appropriate implanted cardioverter defibrillator shock, or cardiovascular death.

CONCLUSIONS:

MYBPC3-related HCM showed increased long-term prevalence of systolic dysfunction compared with MYH7, in spite of similar outcome. Such observations suggest different pathophysiology of clinical progression in the 2 subsets and may prove relevant for understanding of genotype-phenotype correlations in HCM.
From Focal Homonymy to the Ambiguity of Existence (tashkīk al-wujūd): Avicenna’s Reception and Revision of Aristotle’s Categorial Ontology
Authors: Candy Zachary
Published: July 2023

This paper argues that both Aristotle’s theory of the so-called focal homonymy of “being” and Avicenna’s corresponding theory of the ambiguity of “existence” (tashkīk al-wujūd) are meant to address the same dilemma of categorial ontology, but by recourse to different solutions. Avicenna retains Aristotle’s concerns but rejects his solution of focal homonymy, offering a new theory which more satisfactorily addresses each horn of the dilemma. This reading departs from prior scholarship, which, taking Avicenna to have adopted the focal theory basically intact from his Late Antique predecessors, instead tends to privilege the theory’s (peripheral) theological applications.
Relationship closeness as a protective factor against the sensitizing effect of adversity history
Authors: Duong Jacqueline B. , Carta Kayla E. , Walters Sierra N. , Simo Fiallo Natalia , Benamu Dominique I. , Jumonville Grace , Han Sohyun C. , Kim Yehsong , Margolin Gayla , Timmons Adela C.
Published: July 2023

Exposure to adversity has been shown to amplify individuals’ sensitivity to stress, increasing the risk of developing mental health problems in adulthood. Romantic relationships can serve as a broad resource in mitigating the negative impacts of adversity and stress on mental health; however, less is known about how everyday moments of closeness with one’s romantic partner impact experiences of daily stress for people exposed to adversity. The present study uses ecological momentary assessment to examine moment-to-moment relationship closeness as a protective buffer against the effects of stress and adversity history (AH) on mood. Using a mobile app, 109 emerging adult couples ( M age = 23.1) reported hourly feelings of stress, relationship closeness, and mood (i.e., happy, sad, nervous, angry) for one day. Results showed AH and relationship closeness each moderated the association between stress and negative mood. Specifically, greater AH was associated with increased same-hour links between stress and heightened nervousness and anger. Greater relationship closeness was associated with decreased drops in happiness and decreased rises in sadness and anger when stressed. A 3-way interaction between stress, AH, and relationship closeness indicated higher relationship closeness was a buffer against the impact of stress on happiness and anger. Specifically, high relationship closeness mitigated drops in happiness when stressed, especially for those with high AH; relationship closeness also mitigated rises in anger, but this effect was heightened for those with low AH. Exploratory gender moderation analyses showed complex links between stress, AH, closeness, and mood, with evidence indicating enhanced emotional intensity in women when stressed and enhanced protective benefits of relationship closeness in men, although this pattern was inconsistent. The discussion focuses on how experiences of AH can carry forward to shape emotional responses in adults and how relationship closeness may serve as a protective factor against the sensitizing effects of AH.
Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis
Authors: Shi Juan , Wang Qun-Hui , Wei Xiang , Huo Bo , Ye Jian-Nan , Yi Xin , Feng Xin , Fang Ze-Min , Jiang Ding-Sheng , Ma Ming-Jia
Published: July 2023

Background

E1A-associated 300-kDa protein (P300), an endogenous histone acetyltransferase, contributes to modifications of the chromatin landscape of genes involved in multiple cardiovascular diseases. Ferroptosis of vascular smooth muscle cells (VSMCs) is a novel pathological mechanism of aortic dissection. However, whether P300 regulates VSMC ferroptosis remains unknown.

Methods

Cystine deprivation (CD) and imidazole ketone erastin (IKE) were used to induce VSMC ferroptosis. Two different knockdown plasmids targeting P300 and A-485 (a specific inhibitor of P300) were used to investigate the function of P300 in the ferroptosis of human aortic smooth muscle cells (HASMCs). Cell counting kit-8, lactate dehydrogenase and flow cytometry with propidium iodide staining were performed to assess the cell viability and death under the treatment of CD and IKE. BODIPY-C11 assay, immunofluorescence staining of 4-hydroxynonenal and malondialdehyde assay were conducted to detect the level of lipid peroxidation. Furthermore, co-immunoprecipitation was utilized to explore the interaction between P300 and HIF-1α, HIF-1α and P53.

Results

Compared with normal control, the protein level of P300 was significantly decreased in HASMCs treated with CD and IKE, which was largely nullified by the ferroptosis inhibitor ferrostatin-1 but not by the autophagy inhibitor or apoptosis inhibitor. Knockdown of P300 by short-hairpin RNA or inhibition of P300 activity by A-485 promoted CD- and IKE-induced HASMC ferroptosis, as evidenced by a reduction in cell viability and aggravation of lipid peroxidation of HASMCs. Furthermore, we found that hypoxia-inducible factor-1α (HIF-1α)/heme oxygenase 1 (HMOX1) pathway was responsible for the impacts of P300 on ferroptosis of HASMCs. The results of co-immunoprecipitation demonstrated that P300 and P53 competitively bound HIF-1α to regulate the expression of HMOX1. Under normal conditions, P300 interacted with HIF-1α to inhibit HMOX1 expression, while reduced expression of P300 induced by ferroptosis inducers would favor HIF-1α binding to P53 to trigger HMOX1 overexpression. Furthermore, the aggravated effects of P300 knockdown on HASMC ferroptosis were largely nullified by HIF-1α knockdown or the HIF-1α inhibitor BAY87-2243.

Conclusion

Thus, our results revealed that P300 deficiency or inactivation facilitated CD- and IKE-induced VSMC ferroptosis by activating the HIF-1α/HMOX1 axis, which may contribute to the development of diseases related to VSMC ferroptosis.
Minimal important difference, patient acceptable symptom state and longitudinal validity of oxford elbow score and the quickDASH in patients with tennis elbow
Authors: Karjalainen Teemu , Lähdeoja Tuomas , Salmela Mikko , Ardern Clare L , Juurakko Joona , Järvinen Teppo LN , Taimela Simo ,   FINITE investigators
Published: July 2023

Background

The Oxford Elbow Score (OES) and the short version of Disabilities of Arms, Shoulder and Hand (QuickDASH) are common patient-reported outcomes for people with elbow problems. Our primary objective was to define thresholds for the Minimal Important Difference (MID) and Patient-Acceptable Symptom State (PASS) for the OES and QuickDASH. The secondary aim was to compare the longitudinal validity of these outcome measures.

Methods

We recruited 97 patients with clinically-diagnosed tennis elbow for a prospective observational cohort study in a pragmatic clinical setting. Fifty-five participants received no specific intervention, 14 underwent surgery (11 as primary treatment and 4 during follow-up), and 28 received either botulinum toxin injection or platelet rich plasma injection. We collected OES (0 to 100, higher is better) and QuickDASH (0 to 100, higher is worse), and global rating of change (as an external transition anchor question) at six weeks, three months, six months and 12 months. We defined MID and PASS values using three approaches. To assess the longitudinal validity of the measures, we calculated the Spearman’s correlation coefficient between the change in the outcome scores and external transition anchor question, and the Area Under the Curve (AUC) from a receiver operating characteristics (ROC) analysis. To assess signal-to-noise ratio, we calculated standardized response means.

Results

Depending on the method, MID values ranged from 16 to 21 for OES Pain; 10 to 17 for OES Function; 14 to 28 for OES Social-psychological; 14 to 20 for OES Total score, and − 7 to -9 for QuickDASH. Patient-Acceptable Symptom State (PASS) cut offs were 74 to 84 for OES Pain; 88 to 91 for OES Function; 75 to 78 with OES Social-psychological; 80 to 81 with OES Total score and 19 to 23 with Quick-DASH. OES had stronger correlations with the anchor items, and AUC values suggested superior discrimination (between improved and not improved) compared with QuickDASH. OES also had superior signal-to-noise ratio compared with QuickDASH.

Conclusion

The study provides MID and PASS values for OES and QuickDASH. Due to better longitudinal validity, OES may be a better choice for clinical trials.

Trial registration

ClinicalTrials.gov NCT02425982 (first registered April 24, 2015).