bluuebunny/crossref_metadata_2025 huggingface dataset:
Intestinal microbiome-mediated resistance against vibriosis for Cynoglossus semilaevis
Authors: Zhou Qian , Zhu Xue , Li Yangzhen , Yang Pengshuo , Wang Shengpeng , Ning Kang , Chen Songlin
Published: September 2022

Background

Infectious diseases have caused huge economic loss and food security issues in fish aquaculture. Current management and breeding strategies heavily rely on the knowledge of regulative mechanisms underlying disease resistance. Though the intestinal microbial community was linked with disease infection, there is little knowledge about the roles of intestinal microbes in fish disease resistance. Cynoglossus semilaevis is an economically important and widely cultivated flatfish species in China. However, it suffers from outbreaks of vibriosis, which results in huge mortalities and economic loss.

Results

Here, we used C. semilaevis as a research model to investigate the host-microbiome interactions in regulating vibriosis resistance. The resistance to vibriosis was reflected in intestinal microbiome on both taxonomic and functional levels. Such differences also influenced the host gene expressions in the resistant family. Moreover, the intestinal microbiome might control the host immunological homeostasis and inflammation to enhance vibriosis resistance through the microbe-intestine-immunity axis. For example, Phaeobacter regulated its hdhA gene and host cyp27a1 gene up-expressed in bile acid biosynthesis pathways, but regulated its trxA gene and host akt gene down-expressed in proinflammatory cytokines biosynthesis pathways, to reduce inflammation and resist disease infection in the resistant family. Furthermore, the combination of intestinal microbes and host genes as biomarkers could accurately differentiate resistant family from susceptible family.

Conclusion

Our study uncovered the regulatory patterns of the microbe-intestine-immunity axis that may contribute to vibriosis resistance in C. semilaevis. These findings could facilitate the disease control and selective breeding of superior germplasm with high disease resistance in fish aquaculture.
Clinical outcomes of different implant types in mandibular bar-retained overdentures: a retrospective analysis with up to 20 years follow-up
Authors: Betthäuser M. , Schilter R. , Enkling N. , Suter V. G. A. , Abou-Ayash S. , Schimmel M.
Published: September 2022

Purpose

To determine the clinical and radiological outcomes of hybrid-design- (HD) and bone-level (BL) implants for bar-retained mandibular implant-overdentures (IODs).

Methods

For this retrospective study, edentulous patients who had received maxillary complete dentures and mandibular bar-retained IODs were invited for a follow-up assessment. Implant survival, implant success and health of peri-implant tissues were assessed on an implant level-based analysis. Patient-based parameters served to identify risk factors for peri-implant bone loss, presence of peri-implantitis and success.

Results

Eighty patients (median age 72.72 [67.03; 78.81] years, 46 females) with 180 implants (median follow-up 12.01 [10.82; 21.04] years) were assessed. There was no difference concerning the rate of implant failure (p = 0.26), or peri-implantitis (p = 0.97) between HD and BL implants. Solely in one study group, there was the presence of peri-implant pus. Implant success was higher in BL implants with one group being notably higher than the comparing groups (p = 0.045). For bone loss, a width of keratinized mucosa (KM) ≤ 1 mm (p = 0.0006) and the presence of xerostomia (p = 0.09) were identified as risk factors. Smoking (p = 0.013) and a higher body mass index (BMI) (p = 0.03) were a risk factor for peri-implantitis. As risk factors for reduced implant success, a small width of KM (p = 0.003) and the presence of xerostomia (p = 0.007) were identified.

Conclusions

For mandibular bar-retained IODs, both BL and HD implants are mostly successful. A minimum of 1 mm KM around implants and normal salivary flow are relevant factors for implant success and stable peri-implant bone levels. Smoking and a high BMI are potential risk factors for peri-implantitis.
Volvulus of the stomach and wandering spleen after repair of congenital diaphragmatic hernia: unexpected manifestations in a neonate
Authors: Oyachi Noboru , Numano Fuminori , Fukatsu Tamami , Nemoto Atsushi , Naito Atsushi
Published: September 2022

Background

Congenital diaphragmatic hernia (CDH) is sometimes associated with complications involving herniation of intrathoracic organs, which further increase mortality rate. We encountered a case of postoperative gastric and splenic volvulus shortly after left CDH repair in a female neonate who was treated with gastropexy.

Case presentation

At 39 weeks gestation, a female patient with left Bochdalek CDH was delivered (birth weight: 3748 g, Apgar score: 3/4). The patient was provided ventilator support with nitric oxide. After pulmonary hypertension improved, CDH repair was performed via the abdominal approach on day 7. The stomach, small intestine, large intestine, and spleen were herniated through a diaphragmatic defect of 4 × 2 cm. Although the diaphragm was directly closed, it was tight and the reconstructed diaphragm “dome” was shallow, restricting space for the spleen and stomach. Nonetheless, the spleen was positioned in the left upper abdomen and the stomach was positioned medially. The postoperative course was complicated by organo-axial gastric volvulus, and laparotomy was performed on day 14. In addition to the gastric volvulus, we confirmed a wandering splenic volvulus. The spleen was easily detorted and returned to the left upper abdomen. However, the patient experienced relapse of gastric volvulus without splenic volvulus. Gastropexy was performed electively on day 47. Postoperatively, the patient could be fed orally, and the patient’s development was satisfactory 6 years after surgery.

Conclusions

The cause of these rare complications appeared to be tight direct diaphragmatic closure, which reduced space for the spleen and stomach beneath the left diaphragm.
Hyponatremia due to excessive water intake in COVID-19 patients: case series study
Authors: Al-juboori Ahmad Nasrat , Al Hail Amira , Ahmad Al-juboori Zaid
Published: September 2022

Background

Literatures revealed syndrome of inappropriate antidiuretic hormone (SIADH) as the most common mechanism of hyponatremia in COVID-19. However, it is important to rule out other etiologies of hyponatremia.

Methods

This is a case series, retrospective study. Four patients were reviewed from the Mesaieed Hospital, Hamad Medical Corporation, Qatar. The patients were admitted during the second wave of pandemic because of mild to moderate COVID pneumonia. The patients received medications according to the protocol; after few days of treatment, their blood laboratory results showed hyponatremia; as compared to the initial readings, hyponatremia workup excluded SIADH. History revealed that the patients were drinking large amounts of water, around 4–5 L/day, due of certain reasons: one patient had dysgeusia, and the other three patients thought that excessive drinking of water is beneficial for COVID-19 infection.

Results

The hyponatremia level was less than 135 mmol/L, other laboratory tests excluded SIADH, and the provisional diagnosis was dilutional hyponatremia. Male/female ratio was 3/1, age from 29- to 45-year-old patients with no associated comorbidities. Fluid restriction up to 1.5 L/day showed dramatic improvement of their sodium blood level. The patients are discharged in a stable condition.

Conclusions

In COVID-19 patients, hyponatremia not only is secondary to SIADH but can also be due to other etiologies. Hyponatremia can be induced by excessive water drinking and considered an extremely rare reported cases.
Biofeedback pelvic floor muscle training versus posterior tibial nerve electrostimulation in treatment of functional obstructed defecation: a prospective randomized clinical trial
Authors: Saba Emmanuel Kamal Aziz , Elsawy Mervat Sheta
Published: September 2022

Background

Functional obstructed defecation is a common anorectal problem among adult population. The objective was to compare the short-term efficacy of biofeedback pelvic floor muscle training versus transcutaneous posterior tibial nerve electrostimulation in treatment of patients with functional obstructed defecation.

Results

There were 41 patients completed the study. There were no statistical significant differences between biofeedback pelvic floor muscle training group and transcutaneous posterior tibial nerve electrostimulation group regarding different clinical characteristics, as well as, electrophysiological findings. There was statistically significant reduction in all outcome measures after intervention in both groups. The primary outcome measure was Modified obstructed defecation score. Secondary outcome measures were Patient Assessment of Constipation-Quality of Life questionnaire, time of toileting, and maximum anal pressure during straining to evacuate. No significant differences were present between both groups regarding different outcome measures in the pretreatment and post-treatment assessments. Successful outcome was reported in 81% of patients in biofeedback pelvic floor muscle training group in comparison to 40% of patients in the posterior tibial nerve electrostimulation group according to the Modified obstructed defecation score which was the primary outcome measure.

Conclusions

Both biofeedback pelvic floor muscle training and posterior tibial nerve electrostimulation are considered effective methods in the treatment of functional obstructed defecation. However, biofeedback pelvic floor muscle training seems to be more effective and superior in comparison to posterior tibial nerve electrostimulation. Posterior tibial nerve electrostimulation could be combined with biofeedback pelvic floor muscle training or considered as a second line therapy after failure of biofeedback pelvic floor muscle training.

Trial registration

Pan African Clinical Trials Registry, PACTR202009762113535. Registered 2 September 2020—retrospectively registered, https://pactr.samrc.ac.za/TrialDisplay.aspx?TrialID=12321.
Faculty Experiences during the Implementation of an Introductory Biology Course-Based Undergraduate Research Experience (CURE)
Authors: DeChenne-Peters S. E. , Scheuermann N. L.
Published: September 2022

Faculty experiences while implementing a course-based undergraduate research experience (CURE) are explored by interviewing faculty from four diverse institutions. Results indicate that resources, benefits, challenges, and feelings differ before and after CURE implementation and between different institutional types.
Cardiac Arrest Following Remimazolam-Induced Anaphylaxis: A Case Report
Authors: Hasushita Yudai , Nagao Megumi , Miyazawa Yoshihide , Yunoki Kazuma , Mima Hiroyuki
Published: September 2022

Remimazolam is a recently approved benzodiazepine sedative. We report a case of a 72-year-old man who experienced a cardiac arrest due to severe anaphylaxis immediately after general anesthesia induction. Based on the results of skin tests, including those for dextran 40, an excipient in the remimazolam solution, and a review of drugs given during 3 anesthetics, remimazolam was identified as the probable causative agent. Although remimazolam is structurally similar to midazolam, the patient was not allergic to midazolam as demonstrated before and after anaphylaxis. This report highlights the potential risk of allergic reactions to remimazolam.
Lifelines
Authors: Solomon Harris
Published: September 2022

In Lifelines Harris Solomon takes readers into the trauma ward of one of Mumbai’s busiest public hospitals, narrating the stories of the patients, providers, and families who experience and care for traumatic injuries due to widespread traffic accidents. He traces trauma’s moves after the accident: from scenes of road and railway injuries to ambulance interiors; through emergency triage, surgery, and intensive care; and from the morgue for patients who do not survive into the homes of those who do. These pathways reveal how trauma shifts inequalities, infrastructures, and institutions through the lives and labors of clinical spaces. Solomon contends that medicine itself must be understood in terms of lifelines: patterns of embodied movement that determine survival. In reflecting on the centrality of traffic to life, Lifelines explores a fundamental question: How does medicine move us?
Mental health, suicide attempt, and family function for adolescents’ primary health care during the COVID-19 pandemic
Authors: Rojas-Torres Indiana-Luz , Ahmad Mostapha , Martín Álvarez Juan Manuel , Golpe Antonio A , Gil Herrera Richard de Jesús
Published: September 2022

Background: The study’s purpose was to identify associations between mental health risk, suicide attempts, and family function.  

Methods: A correlational, descriptive, and cross-sectional study was carried out in a group of adolescents in the last grade of secondary school to establish the association between mental health risk, suicide attempt, and family functionality. The instruments used were the self-report questionnaire, the suicide risk assessment scale, and the family APGAR. Data analysis was performed using the artificial intelligence algorithm (gower clustering). 

Results: 246 adolescents responded to the three instruments, which made it possible to select those with correlations of sensitive interest and, based on these, an intervention plan. Psychological distress was found in 28%, psychotic symptoms in 85%, and problematic alcohol use in 9%. Good family functioning was identified in 34% and some type of family dysfunction in 66%. In terms of suicide risk, there was a low suicide risk of 74%, 24% medium risk, and 2% high risk. It could be shown that there is a correlation in a group of 15% of the respondents. 

Conclusions: The risk of suffering mental health deterioration and the suicide risk, during this pandemic period, seems to be related to family functionality.
The social prescribing of psychosocial interventions in the treatment of addictions and substance use disorders with military veterans: a reclamation of identity and belonging
Authors: Mottershead Richard
Published: September 2022

Social prescribing is a way of connecting individuals to a source of support within the community to help improve their health and well-being. Social prescribing programmes are being widely promoted within the United Kingdom (UK) and United States as non-pharmaceutical interventions for those living with addiction and substance misuse needs. These needs have been exasperated by the recent COVID-19 pandemic and global economic crisis, with emerging research indicating short-term and long-term detrimental effects on physical and mental health due to substance misuse and addictions. Psychosocial interventions utilize psychological or social factors rather than an overreliance on biological interventions to treat the health impacts of mental illnesses such as addictions and substance use disorder. In this paper, I will discuss the associated determinants of addictions and substance for the military veteran population, as well as how the social prescribing of psychosocial interventions could be used to reaffirm participant’s identity and enhance their sense of belonging for military veterans, using a real-world example in Wales, UK.
Association between depression and sarcopenia in patients with heart failure
Authors: Yuenyongchaiwat Kornanong , Pinijmung Prapromporn , Buakhamsri Adisai
Published: September 2022

Background: Prevalence of heart failure has been increasing worldwide. Decreased muscle mass, poor physical activity, and reduced physical performance or muscle strength might lead to sarcopenia among heart failure patients and therefore lead to low cardiac output, and vice versa. Symptoms of depression are also common mental health problems in heart failure. However, little is known about the prevalence of depressive symptoms in heart failure patients with sarcopenia and the relationship with its. This study aimed to explore the prevalence of and relationship between sarcopenia and depressive symptoms in heart failure patients.

Methods: A cross-sectional study comprised of 150 participants aged 35 years or older. Sarcopenia and no sarcopenia were classified by using the Asian Working Group for Sarcopenia 2019 criteria. Physical performance, muscle strength, and skeletal muscle mass were examined. Symptoms of depression were assessed through utilization of the validated Thai version of Patient Health Questionnaire 9. Results: The prevalence of sarcopenia was 18.67% and depression was 5.33% in heart failure patients. In addition, the prevalence of depression in heart failure patients with sarcopenia were 3.57%. The relationship was observed based on depression scores and New York Heart Association functional classification (r = 0.319, p<.001). Furthermore, heart failure patients with low New York Heart Association functional class had high depression scores (p<.001). However, no significant differences between depression scores and sarcopenia in heart failure patients were identified. Conclusions: Prevalence of sarcopenia in heart failure patients was 18.67% and only 5.33% exhibited depressive symptoms. Only 3.57% exhibited both depression and sarcopenia. Additionally, New York Heart Association functional classification was related to depression.
Bayes factor benefits for clinical psychology: review of child and adolescent evidence base
Authors: Bertelsen Thomas B. , Hoffart Asle , Rekdal Sondre Sverd , Zahl-Olsen Rune
Published: September 2022

Background: Statistical methods are a cornerstone of research in clinical psychology and are used in clinical trials and reviews to determine the best available evidence. The most widespread statistical framework, frequentist statistics, is often misunderstood and misused. Even when properly applied, this framework can lead to erroneous conclusions and unnecessarily prolonged trials. The implications for clinical psychology are difficulties in interpreting best available evidence and unnecessarily costly and burdensome research. An alternative framework, Bayesian statistics, is proposed as a solution to several issues with current practice. Methods: Statistical tests of primary outcome measures were extracted from 272 studies, which were cited in 11 recent reviews in the Evidence-based updates series in the Journal of Clinical Child and Adolescent Psychology. The extracted tests were examined regarding relevant features and re-analyzed using Bayes Factors. Results: When statistical tests were significant, the majority (98%) of re-analyzed tests agreed with such claims. When statistical tests were nonsignificant almost half (43%) of re-analyzed tests disagreed with such claims. Equally important for clinical research, an average of 13% fewer participants per study would have been required if the studies had used Bayes Factors. Conclusions: Bayes Factors offer benefits for research in clinical psychology through intuitive interpretations, and less costly trials.
North Carolina Water Utility Builds Resilience with Distributed Energy Resources
Authors: Anderson Kate , Grymes James , Newman Alexandra , Warren Adam
Published: September 2022

As the frequency and duration of grid outages increase, backup power systems are becoming more important for ensuring that critical infrastructure continues to provide essential services. Most facilities rely on diesel generators, which may be ineffective during long outages owing to limited fuel supplies and high generator failure rates. Distributed energy resources such as solar, storage, and combined-heat-and-power systems, coupled with on-site biofuel production, offer an alternative source of on-site generation that can provide both cost savings and resilience (i.e., the ability to respond to catastrophic events with longer-term consequences). A mixed-integer linear program minimizes costs and maximizes resilience at a wastewater treatment plant in Wilmington, North Carolina. We find that the plant can reduce life-cycle energy costs by 3.1% through the installation of a hybrid combined-heat-and-power, photovoltaic, and storage system. When paired with existing diesel generators, this system can sustain full load for seven days while saving $664,000 over 25 years and reducing diesel fuel use by 48% compared with the diesel-only solution. This analysis informed a decision by the Cape Fear Public Utility Authority to allocate funds for the implementation of a combined-heat-and-power system at the wastewater treatment plant in fiscal year 2023. The benefits of deploying hybrid combined-heat-and-power technologies and the utilization of on-site biofuel production extend, on a national scale, to thousands of wastewater treatment facilities and other types of critical infrastructure.

Funding: This work was supported by the U.S. Department of Energy, Advanced Manufacturing Office [Grant DE-AC36-08GO28308].
Haemothorax in a Pregnant Woman Secondary to Rupture of a Pulmonary Arteriovenous Malformation
Authors: van den Bulck Melissa , Ngah Claude Berenger , Ntula Hyppolyte , Gorur Yilmaz , Cardos Benoît , Brisbois Denis , Lorenzo Villalba Noel , Ali Deeba
Published: September 2022

Pulmonary arteriovenous malformations (PAVMs) are abnormal communications between the arteries and veins of the pulmonary vasculature leading to a right-to-left shunt. We report the case of a pregnant patient who presented with chest pain revealing a haemothorax secondary to the rupture of an arteriovenous malformation. The diagnosis was made during arteriography after simultaneous performance of an urgent caesarean section and thoracic drainage.
The case for copper AM
Authors: Stertz Christian
Published: September 2022

In 2020, Schunk expanded its expertise to include the composite extrusion modelling process (CEM) by acquiring an ExAM 255 multi-material 3D printing system from German 3D printing specialist AIM3D. The first results of this development partnership can now be seen, as Christian Stertz, project manager for systems engineering at Schunk, explains.
Time-difference-of-arrival positioning based on visible light communication for harbor-border inspection
Authors: Feng Renhai , Zhang Zhaolin , Lai Zhimao , Mao Xurui
Published: September 2022

In view of the complexity of port ship supervision and the influence of external factors such as electromagnetic interference in harbor-border inspection, an efficient system combining an unmanned aerial vehicle (UAV) and visible light positioning (VLP) is proposed for locating maritime targets. In this system, a rotatable receiver with five photodetectors (PDs) installed obliquely on UAV is designed for expanding the positioning range and allowing a lower flight altitude. On this basis, we propose the Chan–Taylor (CT) method based on time difference of arrival (TDOA) for target positioning. First, the localization problem is reformulated as a weighted least squares (WLS) problem and provides a good initial estimate via the two-step WLS (TWLS) method. Then, based on Taylor expansion of TDOA equations, estimated error is calculated using the initial estimate, which can correct the estimated position of the target iteratively. To offset the error, weighted centroid CT (WCCT) is proposed by endowing different weights based on error difference to estimated results. For further improving accuracy, a restricted-region fingerprinting positioning based on CT (CT-RFP) is proposed. In restricted area determined by CT, a certain number of fingerprints is generated based on received signal strength (RSS) for matching. Simulation results show that CT is significantly improved over the previous methods. Compared with TWLS, the accuracy of CT is improved by 49.71%. For WCCT, the maximum error is reduced from 8.65 to 6.91 cm, which effectively reduces the influence of error. Moreover, CT-RFP can achieve an accuracy within millimeter level via the appropriate number of fingerprints and ensemble runs of CT, even at high noise levels.
Increased phase precision of spatial light modulators using irrational slopes: application to attosecond metrology
Authors: Harrison Geoffrey R. , Saule Tobias , Davis Brandin , Trallero-Herrero Carlos A.
Published: September 2022

The ability of spatial light modulators (SLMs) to modify the amplitude
and phase of light has proved them invaluable to the optics and
photonics community. In many applications, the bit-depth of SLMs is a
major limiting factor dictated by a digital processor. As a result,
there is usually a compromise between refresh speed and bit-depth.
Here, we present a method to increase the effective bit-depth of SLMs,
which utilizes a linear slope, as is commonly applied to deal with the
zeroth-order effect. This technique was tested using two
interferometric transient absorption spectroscopy setups. Through the
high harmonic generation in gases producing a train of attosecond
pulses and harmonics from solids in the ultraviolet, two pulses are
generated that interfere in the far field providing a measurement of
the optical phase. An increase in the precision far beyond the limit
dictated by the digital processor in the bit-depth was found.
Photoacoustic imaging to monitor outcomes during hyperbaric oxygen therapy: validation in a small cohort and case study in a bilateral chronic ischemic wound
Authors: Mantri Yash , Mishra Aditya , Anderson Caesar A. , Jokerst Jesse V.
Published: September 2022

Hyperbaric oxygen therapy (HBO2) is a common therapeutic modality that drives oxygen into hypoxic tissue to promote healing. Here, ten patients undergoing HBO2 underwent PA oximetry of the left radial artery and forearm pre- and post-HBO2; this cohort validated the use of PA imaging in HBO2. There was a significant increase in radial artery oxygenation after HBO2 (p = 0.002) in the validation cohort. We also include a case study: a non-diabetic male in his 50s (HB 010) presenting with bilateral ischemic and gangrenous wounds. HB 010 showed higher perfusion and oxygen saturation on the right foot than the left after HBO2 which correlated with independent surgical observations. Imaging assisted with limb salvage treatment. Hence, this work shows that PA imaging can measure changes in arterial oxygen saturation due to HBO2; it can also produce 3D maps of tissue oxygenation and evaluate response to therapy during HBO2.
Nonlinear Fourier transform receiver based on a time domain diffractive deep neural network
Authors: Zhou Junhe , Hu Qingsong , Pu Haoqian
Published: September 2022

A diffractive deep neural network (D2NN) is proposed to distinguish the inverse nonlinear Fourier transform (INFT) symbols. Different from other recently proposed D2NNs, the D2NN is fiber based, and it is in the time domain rather than the spatial domain. The D2NN is composed of multiple cascaded dispersive elements and phase modulators. An all-optical back-propagation algorithm is proposed to optimize the phase. The fiber-based time domain D2NN acts as a powerful tool for signal conversion and recognition, and it is used in a receiver to recognize the INFT symbols all optically. After the symbol conversion by the D2NN, simple phase and amplitude measurement will determine the correct symbol while avoiding the time-consuming NFT. The proposed device can not only be implemented in the NFT transmission system, but also in other areas which require all optical time domain signal transformation and recognition, like sensing, signal coding and decoding, beam distortion compensation and image recognition.
Optical-parametric oscillation in photonic-crystal ring resonators
Authors: Black Jennifer A. , Brodnik Grant , Liu Haixin , Yu Su-Peng , Carlson David R. , Zang Jizhao , Briles Travis C. , Papp Scott B.
Published: September 2022

By-design access to laser wavelength, especially with integrated photonics, is critical to advance quantum sensors, such as optical clocks and quantum-information systems, and open opportunities in optical communication. Semiconductor-laser gain provides exemplary efficiency and integration but merely in developed wavelength bands. Alternatively, nonlinear optics requires control of phase matching, but the principle of nonlinear conversion of a pump laser to a designed wavelength is extensible. We report on laser-wavelength access by versatile customization of optical-parametric oscillation (OPO) with a photonic-crystal ring resonator (PhCR). Leveraging the exquisite control of laser propagation provided by a photonic crystal in a traveling-wave ring resonator, we enable OPO generation across a wavelength range of 1234–2093 nm with a 1550-nm pump and 1016–1110 nm with a 1064-nm pump. Moreover, our platform offers pump-to-sideband conversion efficiency of

>

10
%

and negligible additive optical-frequency noise across the output range. From laser design to simulation of nonlinear dynamics, we use a Lugiato–Lefever framework that predicts the system characteristics, including bidirectional OPO generation in the PhCR and conversion efficiency in agreement with our observations. Our experiments introduce broadband lasers by design with PhCR OPOs, providing critical functionalities in integrated photonics.
Computational models reveal how chloride dynamics determine the optimal distribution of inhibitory synapses to minimise dendritic excitability
Authors: Currin Christopher Brian , Raimondo Joseph Valentino
Published: September 2022

Many neurons in the mammalian central nervous system have complex dendritic arborisations and active dendritic conductances that enable these cells to perform sophisticated computations. How dendritically targeted inhibition affects local dendritic excitability is not fully understood. Here we use computational models of branched dendrites to investigate where GABAergic synapses should be placed to minimise dendritic excitability over time. To do so, we formulate a metric we term the “Inhibitory Level” (IL), which quantifies the effectiveness of synaptic inhibition for reducing the depolarising effect of nearby excitatory input. GABAergic synaptic inhibition is dependent on the reversal potential for GABAAreceptors (EGABA), which is primarily set by the transmembrane chloride ion (Cl-) concentration gradient. We, therefore, investigated how variable EGABA and dynamic chloride affects dendritic inhibition. We found that the inhibitory effectiveness of dendritic GABAergic synapses combines at an encircled branch junction. The extent of this inhibitory accumulation is dependent on the number of branches and location of synapses but is independent of EGABA. This inhibitory accumulation occurs even for very distally placed inhibitory synapses when they are hyperpolarising–but not when they are shunting. When accounting for Cl-fluxes and dynamics in Cl-concentration, we observed that Cl-loading is detrimental to inhibitory effectiveness. This enabled us to determine the most inhibitory distribution of GABAergic synapses which is close to–but not at–a shared branch junction. This distribution balances a trade-off between a stronger combinedinhibitory influencewhen synapses closely encircle a branch junction with the deleterious effects of increased Cl-by loading that occurs when inhibitory synapses are co-located.
Investments for effective functionality of health systems towards Universal Health Coverage in Africa: A scoping review
Authors: Karamagi Humphrey Cyprian , Ben Charif Ali , Ngusbrhan Kidane Solyana , Yohanes Tewelde , Kariuki David , Titus Maritza , Batungwanayo Charles , Seydi Aminata Binetou-Wahebine , Berhane Araia , Nzinga Jacinta , Njuguna David , Kipruto Hillary Kipchumba , Andrews Annan Edith , Droti Benson
Published: September 2022

The health challenges in Africa underscore the importance of effectively investing in health systems. Unfortunately, there is no information on systems investments adequate for an effective functional health system. We aimed to address this by conducting a scoping review of existing evidence following the Joanna Briggs Institute Manual for Evidence Synthesis and preregistered with the Open Science Framework (https://osf.io/bvg4z). We included any empirical research describing interventions that contributed to the functionality of health systems in Africa or any low-income or lower-middle-income regions. We searched Web of Science, MEDLINE, Embase, PsycINFO, Cochrane Library, CINAHL, and ERIC from their inception, and hand-searched other relevant sources. We summarized data using a narrative approach involving thematic syntheses and descriptive statistics. We identified 554 unique reports describing 575 interventions, of which 495 reported evidence of effectiveness. Most interventions were undertaken in Africa (80.9%), covered multiple elements of health systems (median: 3), and focused on service delivery (77.4%) and health workforce (65.6%). Effective interventions contributed to improving single (35.6%) or multiple (64.4%) capacities of health systems: access to essential services (75.6%), quality of care (70.5%), demand for essential services (38.6%), or health systems resilience (13.5%). For example, telemedicine models which covered software (technologies) and hardware (health workers) elements were used as a strategy to address issues of access to essential services. We inventoried these effective interventions for improving health systems functionality in Africa. Further analyses could deepen understanding of how such interventions differ in their incorporation of evidence for potential scale across African countries.
Psychometric properties of upper limb kinematics during functional tasks in children and adolescents with dyskinetic cerebral palsy
Authors: Vanmechelen Inti , Bekteshi Saranda , Konings Marco , Feys Hilde , Desloovere Kaat , Aerts Jean-Marie , Monbaliu Elegast
Published: September 2022

Dyskinetic cerebral palsy (DCP) is characterised by involuntary movements, and the movement patterns of children with DCP have not been extensively studied during upper limb tasks. The aim of this study is to evaluate psychometric properties of upper limb kinematics in participants with DCP and typically developing (TD) participants. In current repeatability and validity study, forty individuals with typical development (n = 20) and DCP (n = 20) performed a reach forward/sideways and a reach and grasp task during motion analysis on two occasions. Joint angles at point of task achievement (PTA) and spatio-temporal parameters were evaluated within-and between-sessions using intra-class correlation coefficients (ICC) and standard error of measurement (SEM). Independent t-tests/Mann-Whitney-U tests were used to compare parameters between groups. Within-session ICC values ranged from 0.45 to 1.0 for all parameters for both groups. Within-session SEM values ranged from 1.1° to 11.7° for TD participants and from 1.9° to 13.0° for participants with DCP. Eight within-session repetitions resulted in the smallest change in ICC and SEM values for both groups. Within-session variability was higher for participants with DCP in comparison with the TD group for the majority of the joint angles and spatio-temporal parameters. Intrinsic variability over time was small for all angles and spatio-temporal parameters, whereas extrinsic variability was higher for elbow and scapula angles. Between-group differences revealed lower shoulder adduction and higher elbow flexion, pronation and wrist flexion, as well as higher trajectory deviation and a lower maximal velocity for participants with DCP. This is the first study to assess the psychometric properties of upper limb kinematics in children and adolescents with DCP, showing that children with DCP show higher variability during task execution, requiring a minimum of eight repetitions. However, their variable movement pattern can be reliably captured within-and between-sessions, confirming the potential of three-dimensional motion analysis for assessment of rehabilitation interventions in DCP.
A Clathrin light chain A reporter mouse for in vivo imaging of endocytosis
Authors: Grimm Elisabeth , van der Hoeven Franciscus , Sardella Donato , Willig Katrin I. , Engel Ulrike , Veits Nisha , Engel Robert , Cavalcanti-Adam Elisabetta Ada , Bestvater Felix , Bordoni Luca , Jennemann Richard , Schönig Kai , Schiessl Ina Maria , Sandhoff Roger
Published: September 2022

Clathrin-mediated endocytosis (CME) is one of the best studied cellular uptake pathways and its contributions to nutrient uptake, receptor signaling, and maintenance of the lipid membrane homeostasis have been already elucidated. Today, we still have a lack of understanding how the different components of this pathway cooperate dynamically in vivo. Therefore, we generated a reporter mouse model for CME by fusing eGFP endogenously in frame to clathrin light chain a (Clta) to track endocytosis in living mice. The fusion protein is expressed in all tissues, but in a cell specific manner, and can be visualized using fluorescence microscopy. Recruitment to nanobeads recorded by TIRF microscopy validated the functionality of the Clta-eGFP reporter. With this reporter model we were able to track the dynamics of Alexa594-BSA uptake in kidneys of anesthetized mice using intravital 2-photon microscopy. This reporter mouse model is not only a suitable and powerful tool to track CME in vivo in genetic or disease mouse models it can also help to shed light into the differential roles of the two clathrin light chain isoforms in health and disease.
Dynamic livelihood impacts of COVID-19 on different rural households in mountainous areas of China
Authors: Wang Chengchao , He Xiu , Song Xianqiang , Chen Shanshan , Luo Dongshen
Published: September 2022

Background

The outbreak of COVID-19 pandemic has brought about severe negative livelihood consequences for rural households worldwide. However, the heterogeneity and dynamics of livelihood impacts have been under-researched. There is also lacking a livelihood assessment of the pandemic based on a whole pandemic cycle. This study aimed to investigate the dynamic and heterogeneous livelihood impacts of COVID-19 pandemic for rural households in 2020 based on a case study of Southeast China.

Methods

The pandemic in China had experienced a complete cycle from initial outbreak, to intermediate recovery and finally new normal stage in 2020. We conducted face-to-face interviews with 95 rural households randomly drawn from 2 rural villages in Xunwu County, Jiangxi Province, Southeast China. The sampled households are interviewed with a questionnaire through face-to-face surveys in February and March, 2021 to evaluate the overall livelihood impacts of the pandemic during 2020. The survey collected data on demographic and economic characteristics, governmental control measures, and effects of the COVID-19 on agricultural production, employment, income, education, and daily life. In-depth interviews are also conducted to clarify the livelihood impacts of COVID-19 on villages.

Results

Results showed that the pandemic tremendously caused substantially negative livelihood impacts, including decreasing household income, and disorders in daily lives. The average income loss of all survey households is 6,842 RMB, accounting for 13.01% of the total household income in 2020. Containment measures also resulted in a series of disturbances in daily lives, such as rising food price additional expenditures, travel restrictions, party restrictions, closure of schools and deceasing living standards. There is remarkable household heterogeneity in the livelihood impacts. Results also revealed that the livelihood strategies of rural households to cope with the threat of COVID-19 were different in various pandemic stages.

Conclusion

Our findings have illustrated the severity and heterogeneity of livelihood impacts on rural households induced by COVID-19 pandemic. The dynamics of livelihood impacts is also highlighted in the study. Several policy suggestion was proposed to mitigate these negative consequences of the pandemic.
Endothelial cell-specific loss of eNOS differentially affects endothelial function
Authors: Bu Shuhan , Nguyen Hien C. , Nikfarjam Sepideh , Michels David C. R. , Rasheed Berk , Maheshkumar Sauraish , Singh Shweta , Singh Krishna K.
Published: September 2022

The endothelium maintains and regulates vascular homeostasis mainly by balancing interplay between vasorelaxation and vasoconstriction via regulating Nitric Oxide (NO) availability. Endothelial nitric oxide synthase (eNOS) is one of three NOS isoforms that catalyses the synthesis of NO to regulate endothelial function. However, eNOS’s role in the regulation of endothelial function, such as cell proliferation and migration remain unclear. To gain a better understanding, we genetically knocked down eNOS in cultured endothelial cells using sieNOS and evaluated cell proliferation, migration and also tube forming potential in vitro. To our surprise, loss of eNOS significantly induced endothelial cell proliferation, which was associated with significant downregulation of both cell cycle inhibitor p21 and cell proliferation antigen Ki-67. Knockdown of eNOS induced cell migration but inhibited formation of tube-like structures in vitro. Mechanistically, loss of eNOS was associated with activation of MAPK/ERK and inhibition of PI3-K/AKT signaling pathway. On the contrary, pharmacologic inhibition of eNOS by inhibitors L-NAME or L-NMMA, inhibited cell proliferation. Genetic and pharmacologic inhibition of eNOS, both promoted endothelial cell migration but inhibited tube-forming potential. Our findings confirm that eNOS regulate endothelial function by inversely controlling endothelial cell proliferation and migration, and by directly regulating its tube-forming potential. Differential results obtained following pharmacologic versus genetic inhibition of eNOS indicates a more complex mechanism behind eNOS regulation and activity in endothelial cells, warranting further investigation.
GADF-VGG16 based fault diagnosis method for HVDC transmission lines
Authors: Wu Hao , Yang Yuping , Deng Sijing , Wang Qiaomei , Song Hong
Published: September 2022

Transmission lines are most prone to faults in the transmission system, so high-precision fault diagnosis is very important for quick troubleshooting. There are some problems in current intelligent fault diagnosis research methods, such as difficulty in extracting fault features accurately, low fault recognition accuracy and poor fault tolerance. In order to solve these problems, this paper proposes an intelligent fault diagnosis method for high voltage direct current transmission lines (HVDC) based on Gramian angular difference field (GADF) domain and improved convolutional neural network (VGG16). This method first performs variational modal decomposition (VMD) on the original fault voltage signal, and then uses the correlation coefficient method to select the appropriate intrinsic mode function (IMF) component, and converts it into a two-dimensional image using the Gramian Angular Difference Field(GADF). Finally, the improved VGG16 network is used to extract and classify fault features adaptively to realize fault diagnosis. In order to improve the performance of the VGG16 fault diagnosis model, batch normalization, dense connection and global average pooling techniques are introduced. The comparative experimental results show that the model proposed in this paper can further identify fault features and has a high fault diagnosis accuracy. In addition, the method is not affected by fault type, transitional resistance and fault distance, has good anti-interference ability, strong fault tolerance, and has great potential in practical applications.
Did Covid-19 lockdown positively affect the urban environment and UN- Sustainable Development Goals?
Authors: Nigam Ritwik , Tripathi Gaurav , Priya Tannu , Luis Alvarinho J. , Vaz Eric , Kumar Shashikant , Shakya Achala , Damásio Bruno , Kotha Mahender
Published: September 2022

This work quantifies the impact of pre-, during- and post-lockdown periods of 2020 and 2019 imposed due to COVID-19, with regards to a set of satellite-based environmental parameters (greenness using Normalized Difference Vegetation and water indices, land surface temperature, night-time light, and energy consumption) in five alpha cities (Kuala Lumpur, Mexico, greater Mumbai, Sao Paulo, Toronto). We have inferenced our results with an extensive questionnaire-based survey of expert opinions about the environment-related UN Sustainable Development Goals (SDGs). Results showed considerable variation due to the lockdown on environment-related SDGs. The growth in the urban environmental variables during lockdown phase 2020 relative to a similar period in 2019 varied from 13.92% for Toronto to 13.76% for greater Mumbai to 21.55% for Kuala Lumpur; it dropped to −10.56% for Mexico and −1.23% for Sao Paulo city. The total lockdown was more effective in revitalizing the urban environment than partial lockdown. Our results also indicated that Greater Mumbai and Toronto, which were under a total lockdown, had observed positive influence on cumulative urban environment. While in other cities (Mexico City, Sao Paulo) where partial lockdown was implemented, cumulative lockdown effects were found to be in deficit for a similar period in 2019, mainly due to partial restrictions on transportation and shopping activities. The only exception was Kuala Lumpur which observed surplus growth while having partial lockdown because the restrictions were only partial during the festival of Ramadan. Cumulatively, COVID-19 lockdown has contributed significantly towards actions to reduce degradation of natural habitat (fulfilling SDG-15, target 15.5), increment in available water content in Sao Paulo urban area(SDG-6, target 6.6), reduction in NTL resulting in reducied per capita energy consumption (SDG–13, target 13.3).
Edge effects and vertical stratification of aerial insectivorous bats across the interface of primary-secondary Amazonian rainforest
Authors: Yoh Natalie , Clarke James A. , López-Baucells Adrià , Mas Maria , Bobrowiec Paulo E. D. , Rocha Ricardo , Meyer Christoph F. J.
Published: September 2022

Edge effects, abiotic and biotic changes associated with habitat boundaries, are key drivers of community change in fragmented landscapes. Their influence is heavily modulated by matrix composition. With over half of the world’s tropical forests predicted to become forest edge by the end of the century, it is paramount that conservationists gain a better understanding of how tropical biota is impacted by edge gradients. Bats comprise a large fraction of tropical mammalian fauna and are demonstrably sensitive to habitat modification. Yet, knowledge about how bat assemblages are affected by edge effects remains scarce. Capitalizing on a whole-ecosystem manipulation in the Central Amazon, the aims of this study were to i) assess the consequences of edge effects for twelve aerial insectivorous bat species across the interface of primary and secondary forest, and ii) investigate if the activity levels of these species differed between the understory and canopy and if they were modulated by distance from the edge. Acoustic surveys were conducted along four 2-km transects, each traversing equal parts of primary and ca. 30-year-old secondary forest. Five models were used to assess the changes in the relative activity of forest specialists (three species), flexible forest foragers (three species), and edge foragers (six species). Modelling results revealed limited evidence of edge effects, except for forest specialists in the understory. No significant differences in activity were found between the secondary or primary forest but almost all species exhibited pronounced vertical stratification. Previously defined bat guilds appear to hold here as our study highlights that forest bats are more edge-sensitive than edge foraging bats. The absence of pronounced edge effects and the comparable activity levels between primary and old secondary forests indicates that old secondary forest can help ameliorate the consequences of fragmentation on tropical aerial insectivorous bats.