bluuebunny/crossref_metadata_2025 huggingface dataset:
New Insights on Solvent Implications in the Design of Materials Based on Cellulose Derivatives Using Experimental and Theoretical Approaches
Authors: Filimon Anca , Onofrei Mihaela-Dorina
Published: November 2021

The current paper presents a strategic way to design and develop materials with properties adapted for various applications from biomedicine to environmental applications. In this context, blends of (hydroxypropyl)methyl cellulose (HPMC) and poly(vinylpyrrolidone) (PVP) were obtained to create new materials that can modulate the membrane properties in various fields. Thus, to explore the possibility of using the HPMC/PVP system in practical applications, the solubility parameters in various solvents were initially evaluated using experimental and theoretical approaches. In this frame, the study is aimed at presenting the background and steps of preliminary studies to validate the blends behavior for targeted application before being designed. Subsequently, the analysis of the behavior in aqueous dilute solution of HPMC/PVP blend offers information about the conformational modifications and interactions manifested in system depending on the structural characteristics of polymers (hydrophilicity, flexibility), polymer mixtures composition, and used solvent. Given this background, based on experimental and theoretical studies, knowledge of hydrodynamic parameters and analysis of the optimal compositions of polymer mixtures are essential for establishing the behavior of obtained materials and validation for most suitable applications. Additionally, to guarantee the quality and functionality of these composite materials in the targeted applications, e.g., biomedical or environmental, the choice of a suitable solvent played an important role.
The Influence of CoO/P2O5 Substitutions on the Structural, Mechanical, and Radiation Shielding of Boro-Phosphate Glasses
Authors: Mostafa Ahmed M. A. , Uosif Mohamed A. M. , Alrowaili Ziyad A. , Elsaman Reda , Showahy Ahmed A. , Saddeek Yasser B. , Issa Shams A. M. , Ene Antoaneta , Zakaly Hesham M. H.
Published: November 2021

A new glass system (50−x)P2O5–20B2O3–5Al2O3–25Na2O–xCoO was manufactured using a standard melt quenching procedure, where 1≤ x ≤ 12 mol%. The characteristics of boro-phosphate-glasses containing CoO have been studied. The effect of CoO on the radiation-protective properties of glasses was established. The density of the prepared glasses as a function of CoO increased. XRD was used to check the vitreous structure of samples. Fourier-transform infrared (FTIR) spectroscopy was used to study the structure of each sample. FTIR demonstrated that connections grew as CoO/P2O5 levels increased, and the FTIR spectra shifted to higher wavenumbers. The increment of CoO converts non-bridging oxygens associated with phosphate structural units into bridging oxygens. This process increases the concentration of BO4 structural units and creates new, strong and stable bonds B–O–P, i.e., there is polymerization of the boro-phosphate glass network. With an increase in the ratio of CoO/P2O5 in the produced samples, ultrasonic velocities and elastic moduli were observed to increase. The coefficients of linear and mass attenuation, the transmittance of photons in relation to the photon energy, the efficiency of radiation protection in relation to the photon energy, and the thickness of the absorber were modeled using these two methods (experimental and theoretical). From the obtained values, it can be concluded that the 12Co sample containing 12 mol% will play the most influential role in radiation protection. An increase in the content of cobalt-I oxide led to a significant increase in the linear and mass attenuation coefficient values, which directly contributes to the development of the radiation-protective properties of glass.
Microstructure and Mechanical Properties of Friction Stir Welded 2205 Duplex Stainless Steel Butt Joints
Authors: Ahmed Mohamed M. Z. , Hajlaoui Khalil , El-Sayed Seleman Mohamed M. , Elkady Mahmoud F. , Ataya Sabbah , Latief Fahamsyah H. , Habba Mohamed I. A.
Published: November 2021

Friction stir welding (FSW) as a solid-state process is an excellent candidate for high softening temperature materials welding; however, extending the tool life is required to make the process cost-effective. This work investigates the use of a high pin to shoulder ratio (65%) tungsten carbide (WC) tool for friction stir welding of 5 mm thick 2205 DSS to extend the tool life of this low-cost tool material. In addition, the effect of FSW parameters in terms of rotational rates, travel speeds, and downward forces on the microstructural features and mechanical properties of the welded joints were investigated. Characterization in terms of visual inspection, macro and microstructures, hardness, and tensile testing was conducted. The obtained results indicated that the combined rotational rate, travel speed, and downward force parameters govern the production of defect-free joints. The 2205 DSS friction stir welds show an enhancement in hardness compared to the base material. The stir zone showed a significantly refined grain structure of ferrite and austenite with the reduction in the average grain size from 8.8 µm and 13.3 µm for the base material to 2.71 µm and 2.24 µm, respectively. Moreover, this joint showed higher yield strength and ultimate tensile strength compared to the DSS as-received material.
Method for High-Yield Hydrothermal Growth of Silica Shells on Nanoparticles
Authors: Willinger Max , Felhofer Martin , Reimhult Erik , Zirbs Ronald
Published: November 2021

Coating processes are commonly used in materials science to protect a core or modify material properties. We describe a hydrothermal coating process using TEOS (tetraethyl orthosilicate), a widely used precursor for silica coatings, on three representative template materials (carbon nanotubes, silica, and polystyrene nanoparticles) with different properties and shapes. We compare the efficiency of previously published protocols for silica coatings at room temperature and atmospheric pressure with the hydrothermal process at 160 °C and 3 bar. The hydrothermal method achieves higher yields and thicker silica coatings with the same amount of precursor when compared to the conventional way, thus offering higher effectiveness. Furthermore, the hydrothermal coating process yields more homogeneous shells with a higher density, making hydrothermal coating the method of choice when mechanical integrity and low permeability of the coating are required.
Modulation of the PLLA Morphology through Racemic Nucleation to Reach Functional Properties Required by 3D Printed Durable Applications
Authors: Dimonie Doina , Mathe Silvia , Iftime Manuela Maria , Ionita Daniela , Trusca Roxana , Iftimie Sorina
Published: November 2021

This paper presents an alternative for enhancing the durability of poly (L-lactide) (PLLA) by racemic nucleation following stereo-complexation with a selected poly (D-lactide) (PLDA). The compounds are obtained by melt blending of a PLLA grade, previously designed for 3D printing but with a low heat deflection temperature and impact resistance, with grades of PLDA differing in their molecular weight (Mw), D-lactide content (DS) and concentration. Our method considered how to reveal the racemic nucleation caused by stereo-complexation and its influence on functional properties. The FTIR study we performed showed that, depending on Mw, DS and concentration of the stereo-complexer (PDLA) used, bigger or smaller spectral changes can occur. The stereo-complexation was confirmed by the DSC analysis and, for the selected compound, by the POM, SEM, AFM microscopies, functional property and shapeability as 3D printing filaments. All the obtained results sustain the idea that, if a PLLA with Mw of 4.5 × 104 g·mol−1 is modified with PDLA with a medium Mw of 11.6 × 104 g·mol−1, medium DS of 4% and 1% concentration, a racemic nucleation is possible. It produces a racemic polylactic acid (PDLLA) with improved durability and good shapeability as 3D printing filaments. These results are explicable if the dependence of the intermolecular interactions appears between the PLLA and stereo-complexer PDLA. To enlarge the durable applicability of racemic polylactic acid (PDLLA), future research should identify other parameters controling the PLA stereo-complexing as the intensifying the mobility of the macromolecules, the finding of the optimal recemic cristalization window.
Integrated Design of Spindle Speed Modulation and Cutting Vibration Suppression Controls Using Disturbance Observer for Thread Milling
Authors: Yeh Syh-Shiuh , Chen Chai-Wei
Published: November 2021

In thread milling, there exists a trade-off between thread manufacturing efficiency and thread quality. In this study, an integrated design of spindle speed modulation (SSM) and cutting vibration suppression (CVS) controls using a disturbance observer were developed to simultaneously ensure superior quality and high manufacturing efficiency. The proposed integrated design not only controls the cutting torque while suppressing cutting vibrations but also ensures cost-effectiveness and mitigates the installation problems prevalent in existing sensor-based methods. The SSM control uses a disturbance observer to estimate the cutting torque required on the spindle during thread milling. The estimated cutting torque is used as a feedback signal so that the SSM control can modulate the spindle speed to make the cutting torque achieve a preset torque command. To further avoid cutting vibrations in thread milling, the CVS control analyzes the estimated cutting torque, detects the occurrence of cutting vibrations, and then adjusts the torque command of the SSM control to suppress the cutting vibrations. In this study, thread milling experiments were performed on a computer numerical control milling machine using the workpiece with stacked materials. The feasibility and performance of the proposed integrated design were validated by experiments.
Assessment of the Hardening Behavior and Tensile Properties of a Cold-Rolled Bainitic–Ferritic Steel
Authors: Bassini Emilio , Sivo Antonio , Ugues Daniele
Published: November 2021

The automotive field is continuously researching safer, high-strength, ductile materials. Nowadays, dual-phase (DP) steels are gaining importance, since they meet all these requirements. Dual-phase steel made of ferrite and bainite is the object of a complete microstructural and mechanical characterization, which includes tensile and bending tests. This specific steel contains ferrite and bainite in equal parts; ferrite is the soft phase while bainite acts as a dispersed reinforcing system. This peculiar microstructure, together with fine dispersed carbides, an extremely low carbon content (0.09 wt%), and a minimal degree of strain hardening (less than 10%) allow this steel to compete with traditional medium-carbon single-phase steels. In this work, a full pearlitic C67 steel containing 0.67% carbon was used as a benchmark to build a comparative study between the DP and SP steels. Moreover, the Crussard–Jaoul (C-J) and Voce analysis were adopted to describe the hardening behavior of the two materials. Using the C-J analysis, it is possible to separately analyze the ferrite and bainite strain hardening and understand which alterations occur to DP steel after being cold rolled. On the other hand, the Voce equation was used to evaluate the dislocation density evolution as a function of the material state.
Current Status and Trends of Minimally Invasive Gastrectomy in Korea
Authors: Park Shin-Hoo , Kim Jong-Min , Park Sung-Soo
Published: November 2021

Since its introduction in the early 1990s, laparoscopic gastrectomy has been widely accepted for the treatment of gastric cancer worldwide. In the last decade, the Korean Laparoendoscopic Gastrointestinal Surgery Study group performed important clinical trials and exerted various efforts to enhance the quality of scientific knowledge and surgical techniques in the field of gastric cancer surgery. Laparoscopic gastrectomy has shifted to a new era in Korea due to recent advances and innovations in technology. Here, we discuss the recent updates of laparoscopic gastrectomy—namely, reduced-port, single-incision, robotic, image-guided, and oncometabolic surgery.
Hot Deformation Behavior of a Beta Metastable TMZF Alloy: Microstructural and Constitutive Phenomenological Analysis
Authors: Guerra Ana Paula de Bribean , Jorge Alberto Moreira , Roche Virginie , Bolfarini Claudemiro
Published: November 2021

A metastable beta TMZF alloy was tested by isothermal compression under different conditions of deformation temperature (923 to 1173 K), strain rate (0.172, 1.72, and 17.2 s−1), and a constant strain of 0.8. Stress–strain curves, constitutive constants calculations, and microstructural analysis were performed to understand the alloy’s hot working behavior in regards to the softening and hardening mechanisms operating during deformation. The primary softening mechanism was dynamic recovery, promoting dynamic recrystallization delay during deformation at higher temperatures and low strain rates. Mechanical twinning was an essential deformation mechanism of this alloy, being observed on a nanometric scale. Spinodal decomposition evidence was found to occur during hot deformation. Different models of phenomenological constitutive equations were tested to verify the effectiveness of flow stress prediction. The stress exponent n, derived from the strain-compensated Arrhenius-type constitutive model, presented values that point to the occurrence of internal stress at the beginning of the deformation, related to complex interactions of dislocations and dispersed phases.
Interactions between Dislocations and Penta-Twins in Metallic Nanocrystals
Authors: Chen Yingbin , Huang Qishan , Zhao Shuchun , Zhou Haofei , Wang Jiangwei
Published: November 2021

Dislocation interactions with twin boundary (TB) have been well-established in nanotwinned metals. Penta-twins, as an extreme of crystal twinning, are tacitly assumed to be more effective at blocking dislocation motions than conventional single or coplanar nanotwins. However, the mechanism underlying the interactions between dislocations and penta-twins remains largely unclear. Here, by combining in situ transmission electron microscope (TEM) nanomechanical testing and atomistic simulations, we rationalize the fundamental interactions between dislocations and penta-twins in Au nanocrystals. Our results reveal that the interactions between dislocations and penta-twins show some similar behaviors to the ones in the cases of coplanar nanotwins, including dislocation impedance at TBs, cross-slip into the twinning plane and transmission across the TB. In addition, penta-twins also exhibit some unique behaviors during dislocation interactions, including multiple cross-slip, dislocation-induced core dissociation and climb-induced annihilation/absorption at the penta-twin core. These findings enhance our mechanistic understanding of dislocation behaviors in penta-twins, shedding light on the accessible design of high-performance nanomaterials with multi-twinned nanostructures.
VIS-IR Spectroscopy of Mixtures of Water Ice, Organic Matter, and Opaque Mineral in Support of Small Body Remote Sensing Observations
Authors: Ciarniello Mauro , Moroz Lyuba V. , Poch Olivier , Vinogradoff Vassilissa , Beck Pierre , Rousseau Batiste , Istiqomah Istiqomah , Sultana Robin , Raponi Andrea , Filacchione Gianrico , Kappel David , Pommerol Antoine , Schröder Stefan E. , Pilorget Cedric , Quirico Eric , Mennella Vito , Schmitt Bernard
Published: November 2021

Visual-to-infrared (VIS-IR) remote sensing observations of different classes of outer solar system objects indicate the presence of water ice and organics. Here, we present laboratory reflectance spectra in the 0.5–4.2 μm spectral range of binary particulate mixtures of water ice, organics analogue (kerite), and an opaque iron sulphide phase (pyrrhotite) to investigate the spectral effects of varying mixing ratios, endmember grain size, and mixing modality. The laboratory spectra are also compared to different implementations of the Hapke reflectance model (Hapke, 2012). We find that minor amounts (≲1 wt%) of kerite (investigated grain sizes of 45–63 μm and <25 μm) can remain undetected when mixed in coarse-grained (67 ± 31 μm) water ice, suggesting that organics similar to meteoritic insoluble organic matter (IOM) might be characterized by larger detectability thresholds. Additionally, our measurements indicate that the VIS absolute reflectance of water ice-containing mixtures is not necessarily monotonically linked to water ice abundance. The latter is better constrained by spectral indicators such as the band depths of water ice VIS-IR diagnostic absorptions and spectral slopes. Simulation of laboratory spectra of intimate mixtures with a semi-empirical formulation of the Hapke model suggests that simplistic assumptions on the endmember grain size distribution and shape may lead to estimated mixing ratios considerably offset from the nominal values. Finally, laboratory spectra of water ice grains with fine-grained pyrrhotite inclusions (intraparticle mixture) have been positively compared with a modified version of the Hapke model from Lucey and Riner (2011).
A Combined EPMA and LA-ICP-MS Investigation on Bi-Cu-Au Mineralization from the Kizhnica Ore Field (Vardar Zone, Kosovo)
Authors: Mederski Sławomir , Pršek Jaroslav , Dimitrova Dimitrina , Hyseni Bahri
Published: November 2021

This paper describes a newly discovered Bi-Cu±Au mineralization co-occurring with Pb-Zn-Ag hydrothermal mineralization within the Kizhnica-Hajvalia-Badovc ore field, central Kosovo, Vardar Zone. The mineralogy of two styles of Bi-Cu±Au mineralization was described using EPMA in combination with reflected and transmitted light microscopy. Hydrothermal Cu-Bi veinlets in the Kizhnica andesite quarry consist of Bi sulfosalts (bismuthinite, cosalite, aikinite, and krupkaite), pyrite, hematite, chalcopyrite, galena, sphalerite, and tetrahedrite group minerals. Disseminated Bi-Au-Cu-Te mineralization from the contact type of mineralization (hornfels) consists of Bi sulfosalts (cannizzarite, bismuthinite, galenobismutite, cosalite), associated with sulfarsenides (arsenopyrite, gersdorffite, and cobaltite), base metal sulfides (chalcopyrite, pyrite, sphalerite, pyrrhotite, and galena), native gold, native bismuth, and tetradymite. LA-ICP-MS analyses of sphalerite, chalcopyrite, and tetrahedrite indicate increased content of In and Sn in the Kizhnica Bi-Cu-Au mineralizing system, while LA-ICP-MS analyses in pyrites show the presence of many elements, e.g., Au, As, Co, Sb, Tl, Hg, Pb, Bi related to the structure of pyrite or controlled by nano-inclusions. The results suggest a connection between Bi-Cu±Au mineralization and the proximity to intrusive rocks, which may be helpful for Au exploration in Kosovo.
Protein Hydrolysis by Subcritical Water: A New Perspective on Obtaining Bioactive Peptides
Authors: Rivas-Vela Carlos I. , Amaya-Llano Silvia L. , Castaño-Tostado Eduardo , Castillo-Herrera Gustavo A.
Published: November 2021

The importance of bioactive peptides lies in their diverse applications in the pharmaceutical and food industries. In addition, they have been projected as allies in the control and prevention of certain diseases due to their associated antioxidant, antihypertensive, or hypoglycemic activities, just to mention a few. Obtaining these peptides has been performed traditionally by fermentation processes or enzymatic hydrolysis. In recent years, the use of supercritical fluid technology, specifically subcritical water (SW), has been positioned as an efficient and sustainable alternative to obtain peptides from various protein sources. This review presents and discusses updated research reports on the use of subcritical water to obtain bioactive peptides, its hydrolysis mechanism, and the experimental designs used for the study of effects from factors involved in the hydrolysis process. The aim was to promote obtaining peptides by green technology and to clarify perspectives that still need to be explored in the use of subcritical water in protein hydrolysis.
UHPLC-MS-Based Serum and Urine Metabolomics Reveals the Anti-Diabetic Mechanism of Ginsenoside Re in Type 2 Diabetic Rats
Authors: Wang Heyu , Teng Yaran , Li Shinan , Li Ying , Li Hui , Jiao Lili , Wu Wei
Published: November 2021

Panax ginseng was employed in the treatment of “Xiao-Ke” symptom, which nowadays known as diabetes mellitus, in traditional Chinese medicine for more than a thousand years. Ginsenoside Re was the major pharmacologic ingredient found abundantly in ginseng. However, the anti-diabetic of Ginsenoside Re and its underlying mechanism in metabolic level are still unclear. Serum and urine metabolomic method was carried out to investigate the anti-diabetic pharmacological effects and the potential mechanism of Ginsenoside Re on high-fat diet combined streptozotocin-induced type 2 diabetes mellitus (T2DM) rats based on ultra-high-performance liquid chromatography coupled with quadrupole exactive orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap/MS). Serum and urine samples were collected from the control group (CON), T2DM group, metformin (MET) treatment group, and ginsenoside Re treatment group after intervention. The biochemical parameters of serum were firstly analyzed. The endogenous metabolites in serum and urine were detected by UHPLC-MS. The potential metabolites were screened by multivariate statistical analysis and identified by accurate mass measurement, MS/MS, and metabolite databases. The anti-diabetic-related metabolites were analyzed by KEGG metabolic pathway, and its potential mechanism was discussed. The treatment of ginsenoside Re significantly reduced the blood glucose and serum lipid level improved the oxidative stress caused by T2DM. Biochemical parameters (urea nitrogen, uric acid) showed that ginsenoside Re could improve renal function in T2DM rats. Respective 2 and 6 differential metabolites were found and identified in serum and urine of ginsenoside Re compared with T2DM group and enriched in KEGG pathway. Metabolic pathways analysis indicated that the differential metabolites related to T2DM were mainly involved in arachidonic acid metabolism, Vitamin B6, steroid hormone biosynthesis, and bile secretion metabolic pathways. This study verified the anti-diabetic and anti-oxidation effects of ginsenoside Re, elaborated that ginsenoside Re has a good regulation of the metabolic disorder in T2DM rats, which could promote insulin secretion, stimulated cannabinoid type 1 receptor (CB1), and CaMKK β to activate AMPK signaling pathway, inhibited insulin resistance, and improved blood glucose uptake and diabetic nephropathy, so as to play the role of anti-diabetic.
Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-κB/IRF3 Signaling Pathways
Authors: Mitra Ankita , Ahuja Akash , Rahmawati Laily , Kim Han Gyung , Woo Byoung Young , Hong Yong Deog , Hossain Mohammad Amjad , Zhang Zhiyun , Kim Soo-Yong , Lee Jongsung , Kim Jong-Hoon , Cho Jae Youl
Published: November 2021

Caragana rosea Turcz, which belongs to the Leguminosae family, is a small shrub found in Northern and Eastern China that is known to possess anti-inflammatory properties and is used to treat fever, asthma, and cough. However, the underlying molecular mechanisms of its anti-inflammatory effects are unknown. Therefore, we used lipopolysaccharide (LPS) in RAW264.7 macrophages to investigate the molecular mechanisms that underlie the anti-inflammatory activities of a methanol extract of Caragana rosea (Cr-ME). We showed that Cr-ME reduced the production of nitric oxide (NO) and mRNA levels of iNOS, TNF-α, and IL-6 in a concentration-dependent manner. We also found that Cr-ME blocked MyD88- and TBK1-induced NF-κB and IRF3 promoter activity, suggesting that it affects multiple targets. Moreover, Cr-ME reduced the phosphorylation levels of IκBα, IKKα/β and IRF3 in a time-dependent manner and regulated the upstream NF-κB proteins Syk and Src, and the IRF3 protein TBK1. Upon overexpression of Src and TBK1, Cr-ME stimulation attenuated the phosphorylation of the NF-κB subunits p50 and p65 and IRF3 signaling. Together, our results suggest that the anti-inflammatory activity of Cr-ME occurs by inhibiting the NF-κB and IRF3 signaling pathways.
Antimicrobial Effects of Basil, Summer Savory and Tarragon Lyophilized Extracts in Cold Storage Sausages
Authors: Macari Artur , Sturza Rodica , Lung Ildiko , Soran Maria-Loredana , Opriş Ocsana , Balan Greta , Ghendov-Mosanu Aliona , Vodnar Dan Cristian , Cojocari Daniela
Published: November 2021

The problem of functional foods with bioactive components of natural origin is current for the food industry. Plant extracts rich in polyphenols with antioxidant and antimicrobial activity are a promising source for use in improving the quality and characteristics of fresh meat and meat products. In this context, the purpose of the present study was to evaluate the physico-chemical, microbiological, sensory properties of sausages prepared with the addition of lyophilized extract of basil, thyme or tarragon. For the beginning, the total amount of polyphenols, the antioxidant and antimicrobial activity of the extracts obtained from three spices were evaluated. In the sausages previously infected with Staphylococcus aureus and Escherichia coli it was observed that there is a much larger number of colonies of microorganisms in the control sample compared to the other samples within 24 and 48 h. Moreover, following the addition of sausage extracts, no changes were found regarding their sensory acceptability.
Multifunctional Plasmonic Grating Based on the Phase Modulation of Excitation Light
Authors: Wang Sen , Zhang Jing , Fu Maixia , He Jingwen , Li Xing
Published: November 2021

Multifunctional optical devices are desirable at all times due to their features of flexibility and high efficiency. Based on the principle that the phase of excitation light can be transferred to the generated surface plasmon polaritons (SPPs), a plasmonic grating with three functions is proposed and numerically demonstrated. The Cherenkov SPPs wake or nondiffracting SPPs Bessel beam or focusing SPPs field can be correspondingly excited for the excitation light, which is modulated by a linear gradient phase or a symmetrical phase or a spherical phase, respectively. Moreover, the features of these functions such as the propagation direction of SPPs wake, the size and direction of the SPPs Bessel beam, and the position of SPPs focus can be dynamically manipulated. In consideration of the fact that no extra fabrication is required to obtain the different SPPs fields, the proposed approach can effectively reduce the cost in practical applications.
Photonic Cavity Effects for Enhanced Efficiency in Layered Perovskite-Based Light-Emitting Diodes
Authors: Lin Lyuye , Proietti Zaccaria Remo , Garoli Denis , Krahne Roman
Published: November 2021

Layered architectures for light-emitting diodes (LEDs) are the standard approach for solution-processable materials such as metal-halide perovskites. Upon designing the composition and thicknesses of the layers forming the LED, the primary focus is typically on the optimization of charge injection and balance. However, this approach only considers the process until electrons and holes recombine to generate photons, while for achieving optimized LED performance, the generated light must also be efficiently outcoupled. Our work focuses on the latter aspect. We assume efficient photon generation and analyze the effects of the geometrical configuration together with the dipole orientation, mimicking the light emission, on the main characteristics defining the LED, such as the Purcell effect and the outcoupling efficiency. We find that in-plane dipoles result in significantly increased outcoupling efficiency. Furthermore, the mismatch in refractive index among the layers and their different thicknesses can be tuned to maximize the Purcell effect and minimize internal losses. The combined optimization of dipole orientation and layer thicknesses can improve the efficiency of the LED up to a factor 10, hence highlighting the importance of considering also the photonic properties of the LED structures if the objective is to maximize the LED performance.
High-Temperature Nanoindentation of an Advanced Nano-Crystalline W/Cu Composite
Authors: Burtscher Michael , Zhao Mingyue , Kappacher Johann , Leitner Alexander , Wurmshuber Michael , Pfeifenberger Manuel , Maier-Kiener Verena , Kiener Daniel
Published: November 2021

The applicability of nano-crystalline W/Cu composites is governed by their mechanical properties and microstructural stability at high temperatures. Therefore, mechanical and structural investigations of a high-pressure torsion deformed W/Cu nanocomposite were performed up to a temperature of 600 °C. Furthermore, the material was annealed at several temperatures for 1 h within a high-vacuum furnace to determine microstructural changes and surface effects. No significant increase of grain size, but distinct evaporation of the Cu phase accompanied by Cu pool and faceted Cu particle formation could be identified on the specimen′s surface. Additionally, high-temperature nanoindentation and strain rate jump tests were performed to investigate the materials mechanical response at elevated temperatures. Hardness and Young′s modulus decrease were noteworthy due to temperature-induced effects and slight grain growth. The strain rate sensitivity in dependent of the temperature remained constant for the investigated W/Cu composite material. Also, the activation volume of the nano-crystalline composite increased with temperature and behaved similar to coarse-grained W. The current study extends the understanding of the high-temperature behavior of nano-crystalline W/Cu composites within vacuum environments such as future fusion reactors.
Development of Synthetic DNA Circuit and Networks for Molecular Information Processing
Authors: Zhang Yongpeng , Feng Yuhua , Liang Yuan , Yang Jing , Zhang Cheng
Published: November 2021

Deoxyribonucleic acid (DNA), a genetic material, encodes all living information and living characteristics, e.g., in cell, DNA signaling circuits control the transcription activities of specific genes. In recent years, various DNA circuits have been developed to implement a wide range of signaling and for regulating gene network functions. In particular, a synthetic DNA circuit, with a programmable design and easy construction, has become a crucial method through which to simulate and regulate DNA signaling networks. Importantly, the construction of a hierarchical DNA circuit provides a useful tool for regulating gene networks and for processing molecular information. Moreover, via their robust and modular properties, DNA circuits can amplify weak signals and establish programmable cascade systems, which are particularly suitable for the applications of biosensing and detecting. Furthermore, a biological enzyme can also be used to provide diverse circuit regulation elements. Currently, studies regarding the mechanisms and applications of synthetic DNA circuit are important for the establishment of more advanced artificial gene regulation systems and intelligent molecular sensing tools. We therefore summarize recent relevant research progress, contributing to the development of nanotechnology-based synthetic DNA circuits. By summarizing the current highlights and the development of synthetic DNA circuits, this paper provides additional insights for future DNA circuit development and provides a foundation for the construction of more advanced DNA circuits.
Inherited Developmental and Epileptic Encephalopathies
Authors: Bartolini Emanuele
Published: November 2021

Epileptic encephalopathies often have a genetic etiology. The epileptic activity itself exerts a direct detrimental effect on neurodevelopment, which may add to the cognitive impairment induced by the underlying mutation (“developmental and epileptic encephalopathy”). The focus of this review is on inherited syndromes. The phenotypes of genetic disorders affecting ion channels, metabolic signalling, membrane trafficking and exocytosis, cell adhesion, cell growth and proliferation are discussed. Red flags suggesting family of genes or even specific genes are highlighted. The knowledge of the phenotypical spectrum can indeed prompt the clinician to suspect specific etiologies, expediting the diagnosis.
Targeting HER2 Expressing Tumors with a Potent Drug Conjugate Based on an Albumin Binding Domain-Derived Affinity Protein
Authors: Garousi Javad , Ding Haozhong , von Witting Emma , Xu Tianqi , Vorobyeva Anzhelika , Oroujeni Maryam , Orlova Anna , Hober Sophia , Gräslund Torbjörn , Tolmachev Vladimir
Published: November 2021

Albumin binding domain derived affinity proteins (ADAPTs) are a class of small and folded engineered scaffold proteins that holds great promise for targeting cancer tumors. Here, we have extended the in vivo half-life of an ADAPT, targeting the human epidermal growth factor receptor 2 (HER2) by fusion with an albumin binding domain (ABD), and armed it with the highly cytotoxic payload mertansine (DM1) for an investigation of its properties in vitro and in vivo. The resulting drug conjugate, ADAPT6-ABD-mcDM1, retained binding to its intended targets, namely HER2 and serum albumins. Further, it was able to specifically bind to cells with high HER2 expression, get internalized, and showed potent toxicity, with IC50 values ranging from 5 to 80 nM. Conversely, no toxic effect was found for cells with low HER2 expression. In vivo, ADAPT6-ABD-mcDM1, radiolabeled with 99mTc, was characterized by low uptake in most normal organs, and the main excretion route was shown to be through the kidneys. The tumor uptake was 5.5% ID/g after 24 h, which was higher than the uptake in all normal organs at this time point except for the kidneys. The uptake in the tumors was blockable by pre-injection of an excess of the monoclonal antibody trastuzumab (having an overlapping epitope on the HER2 receptor). In conclusion, half-life extended drug conjugates based on the ADAPT platform of affinity proteins holds promise for further development towards targeted cancer therapy.
Skin-on-a-Chip Technology for Testing Transdermal Drug Delivery—Starting Points and Recent Developments
Authors: Varga-Medveczky Zsófia , Kocsis Dorottya , Naszlady Márton Bese , Fónagy Katalin , Erdő Franciska
Published: November 2021

During the last decades, several technologies were developed for testing drug delivery through the dermal barrier. Investigation of drug penetration across the skin can be important in topical pharmaceutical formulations and also in cosmeto-science. The state-of- the-art in the field of skin diffusion measurements, different devices, and diffusion platforms used, are summarized in the introductory part of this review. Then the methodologies applied at Pázmány Péter Catholic University are shown in detail. The main testing platforms (Franz diffusion cells, skin-on-a-chip devices) and the major scientific projects (P-glycoprotein interaction in the skin; new skin equivalents for diffusion purposes) are also presented in one section. The main achievements of our research are briefly summarized: (1) new skin-on-a-chip microfluidic devices were validated as tools for drug penetration studies for the skin; (2) P-glycoprotein transport has an absorptive orientation in the skin; (3) skin samples cannot be used for transporter interaction studies after freezing and thawing; (4) penetration of hydrophilic model drugs is lower in aged than in young skin; (5) mechanical sensitization is needed for excised rodent and pig skins for drug absorption measurements. Our validated skin-on-a-chip platform is available for other research groups to use for testing and for utilizing it for different purposes.
The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation
Authors: Ramm Ingrid , Sanchez-Fernandez Adrian , Choi Jaeyeong , Lang Christian , Fransson Jonas , Schagerlöf Herje , Wahlgren Marie , Nilsson Lars
Published: November 2021

The addition of glycerol to protein solutions is often used to hinder the aggregation and denaturation of proteins. However, it is not a generalised practice against chemical degradation reactions. The chemical degradation of proteins, such as deamidation and isomerisation, is an important deteriorative mechanism that leads to a loss of functionality of pharmaceutical proteins. Here, the influence of glycerol on the chemical degradation of a protein and its correlation to glycerol-induced conformational changes is presented. The time-dependent chemical degradation of a pharmaceutical protein, GA-Z, in the absence and presence of glycerol was investigated in a stability study. The effect of glycerol on protein conformation and oligomerisation was characterised using asymmetric field-flow fractionation and small-angle neutron scattering in a wide glycerol concentration range of 0–90% v/v. The results from the stability study were connected to the observed glycerol-induced conformational changes in the protein. A correlation between protein conformation and the protective effect of glycerol against the degradation reactions deamidation, isomerisation, and hydrolysis was found. The study reveals that glycerol induces conformational changes of the protein, which favour a more compact and chemically stable state. It is also shown that the conformation can be changed by other system properties, e.g., protein concentration, leading to increased chemical stability.
Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
Authors: Romero-Araya Pablo , Pino Victor , Nenen Ariel , Cárdenas Verena , Pavicic Francisca , Ehrenfeld Pamela , Serandour Guillaume , Lisoni Judit G. , Moreno-Villoslada Ignacio , Flores Mario E.
Published: November 2021

The design of scaffolds to reach similar three-dimensional structures mimicking the natural and fibrous environment of some cells is a challenge for tissue engineering, and 3D-printing and electrospinning highlights from other techniques in the production of scaffolds. The former is a well-known additive manufacturing technique devoted to the production of custom-made structures with mechanical properties similar to tissues and bones found in the human body, but lacks the resolution to produce small and interconnected structures. The latter is a well-studied technique to produce materials possessing a fibrillar structure, having the advantage of producing materials with tuned composition compared with a 3D-print. Taking the advantage that commercial 3D-printers work with polylactide (PLA) based filaments, a biocompatible and biodegradable polymer, in this work we produce PLA-based composites by blending materials obtained by 3D-printing and electrospinning. Porous PLA fibers have been obtained by the electrospinning of recovered PLA from 3D-printer filaments, tuning the mechanical properties by blending PLA with small amounts of polyethylene glycol and hydroxyapatite. A composite has been obtained by blending two layers of 3D-printed pieces with a central mat of PLA fibers. The composite presented a reduced storage modulus as compared with a single 3D-print piece and possessing similar mechanical properties to bone tissues. Furthermore, the biocompatibility of the composites is assessed by a simulated body fluid assay and by culturing composites with 3T3 fibroblasts. We observed that all these composites induce the growing and attaching of fibroblast over the surface of a 3D-printed layer and in the fibrous layer, showing the potential of commercial 3D-printers and filaments to produce scaffolds to be used in bone tissue engineering.
Reimagining Adult Religious Education and Faith Development in a Detraditionalised Ireland
Authors: Sweetman Bernadette
Published: November 2021

The culture of provision of adult religious education and faith development, whereby talks or courses are made available at parish level and/or in formal educational settings, has undoubtedly dominated the Irish scene for many years. The low level of uptake of such opportunities or long-term engagement, however, coupled with the recognised decrease in regular church attendance would suggest that this culture of provision does not meet the needs of the adult population. This mismatch was a key driving force behind the inception of the Adult Religious Education and Faith Development (AREFD) project. Cognisant of cultural and societal changes, a core aim of the project was to assess this traditional culture of provision within a detraditionalised context. The present study is based on data gathered in phase two of the AREFD project consisting of fourteen semi-structured interviews and focus groups conducted between December 2019 and April 2021. The participants were involved for a number of years in adult religious education and faith development in both the Republic of Ireland and Northern Ireland and across a variety of settings. The purpose of these interviews was to gather together the rich insights from the wealth of experience of the interviewees on the practicalities and possibilities central to adult religious education. The findings affirm dissatisfaction amongst participants with the current state of AREFD in Ireland, but indicate that there is hope for the future. Fresh and innovative engagement with adults is called for. This paper outlines key themes emerging from the data which contribute to the conversation of how innovative engagement with adults can revitalise church culture in Ireland.
Electromagnetic Scattering Model for Far Wakes of Ship with Wind Waves on Sea Surface
Authors: Wang Letian , Zhang Min , Liu Jiong
Published: November 2021

A comprehensive electromagnetic scattering model for ship wakes on the sea surface is proposed to study the synthetic aperture radar (SAR) imagery for ship wakes. Our model considers a coupling of various wave systems, including Kelvin wake, turbulent wake, and the ocean ambient waves induced by the local wind. The fluid–structure coupling between the ship and the water surface is considered using the Reynolds–averaged Navier–Stokes (RANS) equation, and the wave–current effect between the ship wake and wind waves is considered using the wave modulation model. The scattering model can better describe the interaction of the ship wakes on sea surface and illustrates well the features of the ship wakes with local wind waves in SAR images.
Spatial Correlation Length Scales of Sea-Ice Concentration Errors for High-Concentration Pack Ice
Authors: Kern Stefan
Published: November 2021

The European Organisation for the Exploitation of Meteorological Satellites-Ocean and Sea Ice Satellite Application Facility–European Space Agency-Climate Change Initiative (EUMETSAT-OSISAF–ESA-CCI) Level-4 sea-ice concentration (SIC) climate data records (CDRs), named SICCI-25km, SICCI-50km and OSI-450, provide gridded SIC error estimates in addition to SIC. These error estimates, called total error henceforth, comprise a random, uncorrelated error contribution from retrieval and sensor noise, aka the algorithm standard error, and a locally-to-regionally correlated contribution from gridding and averaging Level-2 SIC into the Level-4 SIC CDRs, aka the representativity error. However, these CDRs do not yet provide an error covariance matrix. Therefore, correlation scales of these error contributions and the total error in particular are unknown. In addition, larger-scale SIC errors due to, e.g., unaccounted weather influence or mismatch between the actual ice type and the algorithm setup are neither well represented by the total error, nor are their correlation scales known for these CDRs. In this study, I attempt to contribute to filling this knowledge gap by deriving spatial correlation length scales for the total error and the large-scale SIC error for high-concentration pack ice. For every grid cell with >90% SIC, I derive circular one-point correlation maps of 1000 km radius by computing the cross-correlation between the central 31-day time series of the errors and all other 31-day error time series within that circular area (disc) with 1000 km radius. I approximate the observed decrease in the correlation away from the disc’s center with an exponential function that best fits this decrease and thereby obtain the correlation length scale L sought. With this approach, I derive L separately for the total error and the large-scale SIC error for every high-concentration grid cell, and map, present and discuss these for the Arctic and the Southern Ocean for the year 2010 for the above-mentioned products. I find correlation length scales are substantially smaller for the total error, mostly below ~200 km, than the SIC error, ~200 km to ~700 km, in both hemispheres. I observe considerable spatiotemporal variability of the SIC error correlation length scales in both hemispheres and provide first directions to explain these. For SICCI-50km, I present the first evidence of the method’s robustness for other years and time series of L for 2003–2010.
Methodology for Developing a Combined Bathymetric and Topographic Surface Model Using Interpolation and Geodata Reduction Techniques
Authors: Lubczonek Jacek , Wlodarczyk-Sielicka Marta , Lacka Malgorzata , Zaniewicz Grzegorz
Published: November 2021

The research in this paper is concerned with the development of a continuous elevation model in the coastal zones of inland waters. The source data for the creation of numerical terrain models were data acquired by contemporary sensors, such as such as a single-beam echosounder and an unmanned aircraft system. Different interpolation methods were tested in the study. A new approach in the research field is an interpolation method based on the processing of datasets with different degrees of spatial data reduction. The authors call it the Spatial Interpolation Method based on Data Reduction (SIMDR). The choice of method is based on quantitative and qualitative analysis, taking into account the type of interpolation and the method of geodata reduction. A proposal for the practical implementation of the method involves script processing, which automates the processes of modeling and error calculation.
Identifying Surface Urban Heat Island Drivers and Their Spatial Heterogeneity in China’s 281 Cities: An Empirical Study Based on Multiscale Geographically Weighted Regression
Authors: Niu Lu , Zhang Zhengfeng , Peng Zhong , Liang Yingzi , Liu Meng , Jiang Yazhen , Wei Jing , Tang Ronglin
Published: November 2021

The spatially heterogeneous nature and geographical scale of surface urban heat island (SUHI) driving mechanisms remain largely unknown, as most previous studies have focused solely on their global performance and impact strength. This paper analyzes diurnal and nocturnal SUHIs in China based on the multiscale geographically weighted regression (MGWR) model for 2005, 2010, 2015, and 2018. Compared to results obtained using the ordinary least square (OLS) model, the MGWR model has a lower corrected Akaike information criterion value and significantly improves the model’s coefficient of determination (OLS: 0.087–0.666, MGWR: 0.616–0.894). The normalized difference vegetation index (NDVI) and nighttime light (NTL) are the most critical drivers of daytime and nighttime SUHIs, respectively. In terms of model bandwidth, population and Δfine particulate matter are typically global variables, while ΔNDVI, intercept (i.e., spatial context), and NTL are local variables. The nighttime coefficient of ΔNDVI is significantly negative in the more economically developed southern coastal region, while it is significantly positive in northwestern China. Our study not only improves the understanding of the complex drivers of SUHIs from a multiscale perspective but also provides a basis for urban heat island mitigation by more precisely identifying the heterogeneity of drivers.