bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual-band chirality-selective absorbing by plasmonic metasurfaces with breaking mirror and rotational symmetry
Authors: Shen Zhe , Huang Dingxin , Lin Xiaojun
Published: September 2023

In this work, we proposed a state-of-the-art metasurface model that breaks the mirror symmetry and rotation symmetry of the structure. It consists of two-layer rotating gold split rings, and has the capability of chirality-selective absorption for circularly polarized light (CPL) in two bands. The absorption peaks for left- and right- circularly polarized (LCP&RCP) light appeared at 989 nm and 1404 nm, respectively, with the maximum absorptivity of 98.5% and 96.3%, respectively. By changing the rotation angle of the two-layer gold split rings, it could also be designed as a single-band chiral metasurface absorber, which only absorbed RCP light but not LCP light, and the absorptivity of RCP light could be up to 97.4%. Furthermore, we found our designed absorbers had the characteristics of great circular dichroism (CD) and symmetric absorption. The physical mechanism of the selective absorption of CPL by the absorbers may be explained by the current vector analysis. In addition, the absorption peak could be tuned with the changing of the geometrical parameters of the structure. The proposed chirality-selective metasurface absorbers could be used in CD spectral detection, optical communication, optical filtering, and other fields.
Manipulating bipartite and tripartite quantum correlations of mechanical oscillators via optomechanical interaction
Authors: Peng Rui , Yang Zhen , Wang Dawei , Zhou Ling
Published: September 2023

The entanglement of macroscopic mechanical oscillators is always an interesting domain. How to entangle multiple mechanical oscillators is still not well answered. In this paper, we investigate the bipartite and tripartite quantum correlations among three distinct mechanical oscillators interacting with one cavity pumped by a multi-tone driving laser. Floquet cavity modes, resulting from different frequency components of the multi-tone driven cavity, are used to construct channels for quantum correlations between mechanical oscillators. By modulating the effective optomechanical coupling, we can manipulate the mechanical entanglement and EPR steering. The numerical results show that the two-tone driving widely employed is not enough to generate tripartite entanglement, while three- and four-tone driving can be employed to generate and enhance genuine tripartite entanglement. All bipartite entanglement can also be modulated. In addition, we demonstrate the monogamous relation of tripartite EPR steering and manipulate the asymmetry of steering. This work provides a method for manipulating the quantum correlation among multiple macroscopic objects.
Coded aperture snapshot hyperspectral light field tomography
Authors: Zhao Ruixuan , Cui Qi , Wang Zhaoqiang , Gao Liang
Published: September 2023

Multidimensional imaging has emerged as a powerful technology capable of simultaneously acquiring spatial, spectral, and depth information about a scene. However, existing approaches often rely on mechanical scanning or multi-modal sensing configurations, leading to prolonged acquisition times and increased system complexity. Coded aperture snapshot spectral imaging (CASSI) has introduced compressed sensing to recover three-dimensional (3D) spatial-spectral datacubes from single snapshot two-dimensional (2D) measurements. Despite its advantages, the reconstruction problem remains severely underdetermined due to the high compression ratio, resulting in limited spatial and spectral reconstruction quality. To overcome this challenge, we developed a novel two-stage cascaded compressed sensing scheme called coded aperture snapshot hyperspectral light field tomography (CASH-LIFT). By appropriately distributing the computation load to each stage, this method utilizes the compressibility of natural scenes in multiple domains, reducing the ill-posed nature of datacube recovery and achieving enhanced spatial resolution, suppressed aliasing artifacts, and improved spectral fidelity. Additionally, leveraging the snapshot 3D imaging capability of LIFT, our approach efficiently records a five-dimensional (5D) plenoptic function in a single snapshot.
Refractive index and volume fractions of various cellular components of plankton
Authors: Kamowski Michael , Mojica Kristina , Lange Kacey , Zhang Xiaodong
Published: September 2023

The refractive index (RI) of plankton, especially of live cells, has seldom been measured despite its critical role in determining the optical properties of phytoplankton and their effect on underwater light propagation. Here we present measurements of the RI of live cells representing several phytoplankton groups and ciliates collected from field and lab samples using a high precision holo-tomographic microscope, 3D Cell Explorer. The instrument was able to clearly differentiate three separate cellular structures according to their refractive index: membrane, chloroplast for phytoplankton (or cytoplasm for ciliate), and cytosol. RI values for membranes were distinct according to composition; for chloroplasts, were relatively conserved across phytoplankton taxa; and for cytosol, were close to that of seawater. The volumetric fractions of the membrane and chloroplast scale inversely with cell size whereas the volume of cytosol increased logistically. These results provide a more accurate measurement of RI and volume fractions of various cellular structures that can be used to improve optical modeling of marine plankton.
Soft glass optical fiber characterization with X-ray computed microtomography
Authors: Crocco Maria C. , Mangini Fabio , Filosa Raffaele , Solano Andrea , Agostino Raffaele G. , Barberi Riccardo C. , Couderc Vincent , Klimczak Mariusz , Filipkowski Adam , Buczynski Ryszard , Wabnitz Stefan , Formoso Vincenzo , Ferraro Mario
Published: September 2023

The high loss due to the presence of strong phonon resonances makes silica fibers unfit for applications in the mid-infrared spectral range. This has led to the development of specialty optical fibers, based on novel materials and manufacturing techniques. In some cases, the characterization of these new fibers by means of standard techniques may be challenging. Fiber manufacturers would strongly benefit from a tool, which is capable of checking the geometrical and optical properties of fibers (either after fiber drawing, or even in real-time, during the drawing process). Here, we propose and demonstrate that absorption contrast X-ray computed microtomography is a non-destructive technique, capable of characterizing both geometrical and optical properties of specialty optical fibers. We experimentally verified that the tomographic intensity profile in the fiber core has the same shape as the refractive index profile, which we determined via energy-dispersed X-ray spectroscopy. We tested step- and graded-index soft glass fibers, both purchased and made in-house. Owing to the presence of high atomic number elements, which provide higher X-ray cross-section, soft glasses were more suitable than silica for their characterization via X-ray computed microtomography.
CIRCUST: A novel methodology for temporal order reconstruction of molecular rhythms; validation and application towards a daily rhythm gene expression atlas in humans
Authors: Larriba Yolanda , Mason Ivy C. , Saxena Richa , Scheer Frank A. J. L. , Rueda Cristina
Published: September 2023

The circadian system drives near-24-h oscillations in behaviors and biological processes. The underlying core molecular clock regulates the expression of other genes, and it has been shown that the expression of more than 50 percent of genes in mammals displays 24-h rhythmic patterns, with the specific genes that cycle varying from one tissue to another. Determining rhythmic gene expression patterns in human tissues sampled as single timepoints has several challenges, including the reconstruction of temporal order of highly noisy data. Previous methodologies have attempted to address these challenges in one or a small number of tissues for which rhythmic gene evolutionary conservation is assumed to be preserved. Here we introduce CIRCUST, a novel CIRCular-robUST methodology for analyzing molecular rhythms, that relies on circular statistics, is robust against noise, and requires fewer assumptions than existing methodologies. Next, we validated the method against four controlled experiments in which sampling times were known, and finally, CIRCUST was applied to 34 tissues from the Genotype-Tissue Expression (GTEx) dataset with the aim towards building a comprehensive daily rhythm gene expression atlas in humans. The validation and application shown here indicate that CIRCUST provides a flexible framework to formulate and solve the issues related to the analysis of molecular rhythms in human tissues. CIRCUST methodology is publicly available at https://github.com/yolandalago/CIRCUST/.
A double-negative feedback loop between miR319c and JAW-TCPs establishes growth pattern in incipient leaf primordia in Arabidopsis thaliana
Authors: Shankar Naveen , Sunkara Preethi , Nath Utpal
Published: September 2023

The microRNA miR319 and its target JAW-TCP transcription factors regulate the proliferation-to-differentiation transition of leaf pavement cells in diverse plant species. In young Arabidopsis leaf primordia, JAW-TCPs are detected towards the distal region whereas the major mRNA319-encoding gene MIR319C, is expressed at the base. Little is known about how this complementary expression pattern of MIR319C and JAW-TCPs is generated. Here, we show that MIR319C is initially expressed uniformly throughout the incipient primordia and is later abruptly down-regulated at the distal region, with concomitant distal appearance of JAW-TCPs, when leaves grow to ~100 μm long. Loss of JAW-TCPs causes distal extension of the MIR319C expression domain, whereas ectopic TCP activity restricts MIR319C more proximally. JAW-TCPs are recruited to and are capable of depositing histone H3K27me3 repressive marks on the MIR319C chromatin. JAW-TCPs fail to repress MIR319C in transgenic seedlings where the TCP-binding cis-elements on MIR319C are mutated, causing miR319 gain-of-function-like phenotype in the embryonic leaves. Based on these results, we propose a model for growth patterning in leaf primordia wherein MIR319C and JAW-TCPs repress each other and divide the uniformly growing primordia into distal differentiation zone and proximal proliferation domain.
Discovery of long non-coding RNAs in the liver fluke, Fasciola hepatica
Authors: McVeigh Paul , McCammick Erin , Robb Emily , Brophy Peter , Morphew Russell M. , Marks Nikki J. , Maule Aaron G.
Published: September 2023

Long non-coding (lnc)RNAs are a class of eukaryotic RNA that do not code for protein and are linked with transcriptional regulation, amongst a myriad of other functions. Using a custom in silico pipeline we have identified 6,436 putative lncRNA transcripts in the liver fluke parasite, Fasciola hepatica, none of which are conserved with those previously described from Schistosoma mansoni. F. hepatica lncRNAs were distinct from F. hepatica mRNAs in transcript length, coding probability, exon/intron composition, expression patterns, and genome distribution. RNA-Seq and digital droplet PCR measurements demonstrated developmentally regulated expression of lncRNAs between intra-mammalian life stages; a similar proportion of lncRNAs (14.2%) and mRNAs (12.8%) were differentially expressed (p<0.001), supporting a functional role for lncRNAs in F. hepatica life stages. While most lncRNAs (81%) were intergenic, we identified some that overlapped protein coding loci in antisense (13%) or intronic (6%) configurations. We found no unequivocal evidence for correlated developmental expression within positionally correlated lncRNA:mRNA pairs, but global co-expression analysis identified five lncRNA that were inversely co-regulated with 89 mRNAs, including a large number of functionally essential proteases. The presence of micro (mi)RNA binding sites in 3135 lncRNAs indicates the potential for miRNA-based post-transcriptional regulation of lncRNA, and/or their function as competing endogenous (ce)RNAs. The same annotation pipeline identified 24,141 putative lncRNAs in F. gigantica. This first description of lncRNAs in F. hepatica provides an avenue to future functional and comparative genomics studies that will provide a new perspective on a poorly understood aspect of parasite biology.
Protocol for a cluster randomised waitlist-controlled trial of a goal-based behaviour change intervention for employees in workplaces enrolled in health and wellbeing initiatives
Authors: Alidu Lailah , Al-Khudairy Lena , Bharatan Ila , Bird Paul , Campbell Niyah , Currie Graeme , Hemming Karla , Jolly Kate , Kudrna Laura , Lilford Richard , Martin James , Quinn Laura , Schmidtke Kelly Ann , Yates James ,   On behalf of the Prevention Workplace Collaboration West Midlands
Published: September 2023

Many workplaces offer health and wellbeing initiatives to their staff as recommended by international and national health organisations. Despite their potential, the influence of these initiatives on health behaviour appears limited and evaluations of their effectiveness are rare. In this research, we propose evaluating the effectiveness of an established behaviour change intervention in a new workplace context. The intervention, ‘mental contrasting plus implementation intentions’, supports staff in achieving their health and wellbeing goals by encouraging them to compare the future with the present and to develop a plan for overcoming anticipated obstacles. We conducted a systematic review that identified only three trials of this intervention in workplaces and all of them were conducted within healthcare organisations. Our research will be the first to evaluate the effectiveness of mental contrasting outside a solely healthcare context. We propose including staff from 60 organisations, 30 in the intervention and 30 in a waitlisted control group. The findings will contribute to a better understanding of how to empower and support staff to improve their health and wellbeing.

Trial registration: ISRCTN17828539.
The impact of trade on employment: New evidence from a global value chains perspective
Authors: Mingyang Yue , Hankun Yuan , Chen Xu , Zhida Jin
Published: September 2023

Given the magnitude impact of global value chains (GVCs) in reconstructing the pattern of world trade, its employment implications deserve thorough study. In this paper we explore the impact of GVCs position on employment across countries and its heterogeneous mechanisms. We perform an in-depth theoretical analysis followed by an empirical test using panel data for 56 industries in 42 countries from 2000 to 2014. The results show that enhancing the GVCs position will significantly increase employment, with a more pronounced effect in developing countries. Mechanical tests demonstrate a positive wage effect for developed countries. For developing countries, there is a positive demand effect and a negative factor substitution effect. Heterogeneity tests show that developed countries promote employment primarily by improving the forward GVCs position. Developing countries boost employment mainly by reducing the backward GVCs position. Further research has revealed that employment in developed countries has a clear preference for industries with higher GVCs, crowding out employment in other industries. This paper has enriched research on the employment implications of GVCs position and exploring the possible crowding effect during the evolution of the position of GVCs, which has been informative and insightful for countries in formulating GVCs participation and employment policies.
Consequences of Type-2 diabetes mellitus and Malaria co-morbidity on sperm parameters in men; a case-control study in a district hospital in the Ashanti Region of Ghana
Authors: Abdulai Ratif , Akwetey Samuel Addo , Ogunbode Olayinka Oladunjoye , Aboagye Benjamin
Published: September 2023

Type-2 diabetes mellitus (T2DM) and malaria infection are highly prevalent in Africa particularly, in the Sub-Saharan Region. A greater number of people in the Ghanaian population who have T2DM are also reported to harbor malaria parasites. This study aimed to investigate the influence of T2DM & Malaria co-morbidity on sperm parameters among patients in the Ashanti Region of Ghana. This hospital-based cross-sectional analytic case-control study comprised 254 adult male study participants comprising 80 T2DM & Malaria co-morbidity, 80 T2DM only, and 94 normal controls. A blood sample (10mL) was drawn from each participant to measure FBG, HbA1c levels, Testosterone levels, Total cholesterol, and determination of Malaria parasite density. Seminal fluid was also collected from each participant for semen analysis. Sperm kinetics of the T2DM & Malaria co-morbidity group particularly; total motility, rapid progressive motility, and slow progressive motility were negatively affected compared to both T2DM only (p<0.0001) and the Normal control (p<0.0001). Normal sperm morphology was significantly affected in the co-morbidity group compared to T2DM only (p<0.0001). Sperm vitality was also statistically significantly reduced in the T2DM & Malaria co-morbidity than in T2DM only (t(64)= -8.62; p<0.001). There was a significant decline in testosterone levels in the T2DM & Malaria co-morbidity group than in the T2DM only (p<0.0001) and the control (p <0.0001). In conclusion, T2DM and malaria infection have a stronger propensity to alter sperm morphology and lower sperm motility and vitality.
Investigation on physical properties and modification mechanisms of diatomite/SBR modified asphalt
Authors: Li Di , Chen Rui
Published: September 2023

In recent years, diatomite has been successfully adopted in asphalt modification to overcome the problems of polymer modified asphalt, because of the advantages in wide sources, low price, and worthy technical characteristics. Although the improvement of the high-temperature performance of the modified diatomite asphalt has been verified in previous studies, the diatomite will bring negative impact on the low-temperature resistance. Hence, the objective of this study is to seek a new channel to improve the comprehensive performance of the diatomite modified asphalt binder. Considering the advantage of the SBR in improving the low-temperature performance of asphalt binder, the diatomite/SBR composite modified asphalt binder (DSA) and the corresponding preparation technology are developed to obtain an improved comprehensive performance via the orthogonal experiment method in this study. Moreover, the modification mechanisms of the DSA are revealed using fluorescence microscopy (FM) tests, Gel permeation chromatography (GPC) tests, and Fourier transform infrared spectroscopy (FTIR) tests.
Light therapy for sleep disturbance comorbid depression in relation to neural circuits and interactive hormones—A systematic review
Authors: Yaodong Chen , Zhang Yingzi , Feng Guo , Lei Yuanfang , Liu Qiuping , Liu Yang
Published: September 2023

Aim

To provide an overview of the evidence on the effect of light therapy on sleep disturbance and depression, identify the light-active neural and hormonal correlates of the effect of light therapy on sleep disturbance comorbid depression (SDCD), and construct the mechanism by which light therapy alleviates SDCD.

Methods

Articles published between 1981 and 2021 in English were accessed using Science Direct, Elsevier, and Google Scholar following a three-step searching process via evolved keywords. The evidence level, reliability, and credibility of the literature were evaluated using theevidence pyramidmethod, which considers the article type, impact factor, and journal citation report (JCR) partition.

Results

A total of 372 articles were collected, of which 129 articles fit the inclusion criteria and 44% were at the top of the evidence pyramid hierarchy; 50% were in the first quarter of the JCR partitions. 114 articles provided specific neural and hormonal evidence of light therapy and were further divided into three groups: 37% were related to circadian regulation circuits, 27% were related to emotional regulation circuits, and 36% were related to hormones.

Conclusions

First, neural and hormonal light-active pathways for alleviating sleep disturbance or depression were identified, based on which the neural correlates of SDCD were located. Second, the light responses and interactions of hormones were reviewed and summarized, which also provided a way to alleviate SDCD. Finally, the light-active LHb and SCN exert extensive regulation impacts on the circadian and emotional circuits and hormones, forming a dual-core system for alleviating SDCD.
Move it or lose it: Predicted effects of culverts and population density on Mojave desert tortoise (Gopherus agassizii) connectivity
Authors: Dutcher Kirsten E. , Nussear Kenneth E. , Heaton Jill S. , Esque Todd C. , Vandergast Amy G.
Published: September 2023

Roadways and railways can reduce wildlife movements across landscapes, negatively impacting population connectivity. Connectivity may be improved by structures that allow safe passage across linear barriers, but connectivity could be adversely influenced by low population densities. The Mojave desert tortoise is threatened by habitat loss, fragmentation, and population declines. The tortoise continues to decline as disturbance increases across the Mojave Desert in the southwestern United States. While underground crossing structures, like hydrological culverts, have begun receiving attention, population density has not been considered in tortoise connectivity. Our work asks a novel question: How do culverts and population density affect connectivity and potentially drive genetic and demographic patterns? To explore the role of culverts and population density, we used agent-based spatially explicit forward-in-time simulations of gene flow. We constructed resistance surfaces with a range of barriers to movement and representative of tortoise habitat with anthropogenic disturbance. We predicted connectivity under variable population densities. Simulations were run for 200 non-overlapping generations (3400 years) with 30 replicates using 20 microsatellite loci. We evaluated population genetic structure and diversity and found that culverts would not entirely negate the effects of linear barriers, but gene flow improved. Our results also indicated that density is important for connectivity. Low densities resulted in declines regardless of the landscape barrier scenario (> 75% population census size, > 97% effective population size). Results from our simulation using current anthropogenic disturbance predicted decreased population connectivity over time. Genetic and demographic effects were detectable within five generations (85 years) following disturbance with estimated losses in effective population size of 69%. The pronounced declines in effective population size indicate this could be a useful monitoring metric. We suggest management strategies that improve connectivity, such as roadside fencing tied to culverts, conservation areas in a connected network, and development restricted to disturbed areas.
Non-invasive assessment of steatohepatitis indicates increased risk of coronary artery disease
Authors: Beer Sebastian , Babel Jonas , Martin Neef , Blank Valentin , Wiegand Johannes , Karlas Thomas
Published: September 2023

Introduction

Fatty liver diseases (FLD), especially defined as metabolic dysfunction-associated FLD (MAFLD), is of growing importance for patients and health-care providers. Extrahepatic comorbidities, predominantly coronary artery disease (CAD), contribute to excess morbidity and mortality in FLD. Although the association of FLD and CAD is well known, underlying pathophysiological links are not fully understood. Non-invasive means of liver diagnostic enable a fast and thorough characterization of FLD. We therefore assessed the severity of FLD in a cohort of patients at risk of CAD.

Methods

Patients scheduled for coronary angiography were characterized by anthropometry, serum-based indices of liver fibrosis (NFS, FIB4), abdominal ultrasound and vibration controlled transient elastography (VCTE) including controlled attenuation parameter (CAP) and the Fibroscan-AST (FAST) score. Patients were stratified according to indication of therapeutic coronary intervention.

Results

120 patients were recruited, MAFLD was found in 41%, while advanced fibrosis or cirrhosis were present in only 5%. Coronary vascular intervention was indicated in 42% (n = 50). Severity of steatosis assessed by CAP and risk of fibrosis defined by elevated liver stiffness (VCTE>8 kPa) and fibrosis indices were associated with the need for coronary intervention. FAST score, a marker of fibrotic steatohepatitis, was elevated in the intervention group (0.22 vs. 0.12, p<0.001). Multivariate regression analysis revealed FAST score as strongest predictor of CAD (OR 2.3 95%, CI 1.40–2.96).

Discussion

MAFLD is a frequent comorbidity in patients at CAD risk, but advanced liver disease has a low prevalence in patients undergoing elective coronary angiography. Therefore, a routine VCTE-based screening for FLD cannot be recommended in cardiac patients. The association of indicators of steatohepatitis with advanced CAD points to inflammatory processes as a conjoint mechanism of both diseases.
Lifestyle and Sense of Coherence: A comparative analysis among university students in different areas of knowledge
Authors: Braga João Paulo Costa , Wolfgram Eduardo , Batista de Souza João Paulo , Fausto Silva Larissa Gabriele , Estavien Yonel , de Almeida Roberto , Pestana Cezar Rangel
Published: September 2023

Background

The concept of health has undergone profound changes. Lifestyle Medicine consists of therapeutic approaches that focus on the prevention and treatment of diseases. It follows that the quality of life of university students directly affects their health and educational progress.

Experimental methodology

Socioeconomic, lifestyle (LS), and Salutogenesis Theory/sense of coherence (SOC) questionnaires were administered to college students from three different areas. The results were analyzed for normality and homogeneity, followed by ANOVA variance analysis and Dunn and Tukey post hoc test for multiple comparisons. Spearman’s correlation coefficient evaluated the correlation between lifestyle and sense of coherence; p values < 0.05 were considered statistically significant.

Results

The correlation between LS and SOC was higher among males and higher among Medical and Human sciences students compared to Exact sciences. Medical students’ scores were higher than Applied sciences and Human sciences students on the LS questionnaire. Exact science students’ scores on the SOC questionnaire were higher than Human sciences students. In the LS areas related to alcohol intake, sleeping quality, and behavior, there were no differences between the areas. However, women scored better in the nutrition domain and alcohol intake. The SOC was also higher in men compared to women.

Conclusion

The results obtained demonstrate in an unprecedented way in the literature that the correlation between the LS and SOC of college students varies according to gender and areas of knowledge, reflecting the importance of actions on improving students’ quality of life and enabling better academic performance.
Musculoskeletal model of osseointegrated transfemoral amputees in OpenSim
Authors: Raveendranathan Vishal , Kooiman Vera G. M. , Carloni Raffaella
Published: September 2023

This study presents a generic OpenSim musculoskeletal model of people with an osseointegrated unilateral transfemoral amputation wearing a generic prosthesis. The model, which consists of seventy-six musculotendon units and two ideal actuators at the knee and ankle joints of the prosthesis, is tested by designing an optimal control strategy that guarantees the tracking of experimental amputee data during level-ground walking while finding the actuators’ torques and minimizing the muscle forces. The model can be made subject-specific and, as such, is able to reproduce the kinematics and dynamics of both healthy and amputee subjects. The model provides a tool to analyze the biomechanics of level-ground walking and to understand the contribution of the muscles and of the prosthesis’ actuators. The proposed OpenSim musculoskeletal model is released as support material to this study.
Crossover of the dimensions of work-family and family-work conflict in couples: Protocol for a qualitative study
Authors: Smoktunowicz Ewelina , Lesnierowska Magdalena , Ziolkowska Justyna , Roczniewska Marta
Published: September 2023

Conflict between work and non-work is a bidirectional and a multidimensional construct that has garnered much attention from researchers and practitioners alike. Previously, studies with a dyadic design demonstrated that interrole conflict can cross over between partners in romantic relationships. The aim of the present study is to explore—from an individual and dyadic perspective—how partners perceive dimensions of interrole conflict (that is: time, strain, behaviour, and possibly others) and whether crossover between partners is dimension-dependent. This protocol outlines a qualitative interview study. Participants (N = 40) will be dual-earner couples that meet two inclusion criteria: both partners need to be professionally active, and the couples need to have lived together for at least a year. Interviews will be conducted separately with each partner. To analyse the data at the individual level we will use reflexive thematic analysis. To analyse the data at the dyadic level we will apply an adapted version of the framework method. We anticipate that findings of this study will have the potential to advance theoretical models depicting crossover processes and, more generally, the interface between work and family lives. Moreover, insights into how couples experience dimension-based interrole conflict will be important for the development of targeted interventions.
Honey bee retinue workers respond similarly to queens despite seasonal differences in Queen Mandibular Pheromone (QMP) signaling
Authors: Carroll Mark J. , Brown Nicholas J. , Ruetz Zachary , Ricigliano Vincent A. , Anderson Kirk E.
Published: September 2023

Honey bee colonies maintain viable queens in part through communication with Queen Mandibular Pheromone (QMP), a mixture that signals the queen’s presence and reproductive quality to workers. In turn, workers are thought to provide retinue queen care or replace queens partially based on QMP profiles. We examined the effects of seasonal dearth (overwintering in a warm subtropical location) on queen-worker interactions. Retinue worker responses to continuously ovipositing queens were considered in view of QMP signaling and queen reproductive quality. QMP signaling was estimated from QMP residues recovered from nest worker bodies, which is the primary mode of QMP transfer from the queen to the colony at large. QMP residues varied seasonally but not at all with queen reproductive quality (spermatheca sperm storage, ovary protein and lipid contents). 9-HDA and 9-ODA were lower in January than other months. HOB decreased from July to January, while HVA, a component associated with mated queens, increased sharply in January. Despite these seasonal signaling differences, retinue workers attended queens at similar levels through the months. In terms of reproductive quality, queens did not differ over the months in matedness (spermatheca sperm storage) or physiological age (protein carbonyl content), but varied in nutrient allocation to reproductive and non-reproductive tissues. Queen ovaries contained more protein in September than in November, and more lipid in July and September than in November and January. Queen fat bodies had more protein in July than September or November, but less lipid in July and September than November or January. Retinue worker responses did not vary with seasonal QMP changes, but reflected overall continuous brood rearing efforts and queen matedness throughout the year. The absence of seasonal differences in worker responses to QMP should be considered in the broader context of continuous reproductive efforts in warm subtropical colonies.
Mitogenomic analysis and phylogenetic relationships of Agrilinae: Insights into the evolutionary patterns of a diverse buprestid subfamily
Authors: Huang Xuyan , Wei Zhonghua , Lu Jiawei , Shi Aimin
Published: September 2023

Agrilinae is the largest subfamily in Buprestidae, which includes the four tribes, namely Coraebini, Agrilini, Aphanisticini, and Tracheini. However, there is a need to verify the evolutionary relationships among the taxa in Buprestidae. Thus, to explore the phylogenetic position of Aphanisticini, the mitochondrial genomes of Endelus continentalis and Cantonius szechuanensis were sequenced using next-generation sequencing technology. Three other mitogenomes of agriline beetles, Agrilus discalis, Sambus kanssuensis, and Habroloma sp., were also sequenced for the phylogenetic analyses. The divergence time of Buprestidae was estimated based on the mitogenomes. The general features of the known mitogenomes of Agrilinae were compared, analyzed, and summarized. Out of these five species, S. kanssuensis had the shortest mitogenome length (15,411), while Habroloma sp. had the longest (16,273). The gene arrangement of the five new sequences was identical to that of the reported buprestid mitogenomes. The Ka/Ks ratios of Meliboeus (0.79) and Endelus (0.78) were significantly larger than those of the other agriline genera. The results of the phylogeny indicated that Aphanisticini was more closely related to Tracheini and that the genus Sambus separated from the base of the Agrilinae clade at about 130 Ma. Moreover, Aphanisticini and Tracheini diverged at around 26 Ma.
High accurate and explainable multi-pill detection framework with graph neural network-assisted multimodal data fusion
Authors: Nguyen Anh Duy , Pham Huy Hieu , Trung Huynh Thanh , Nguyen Quoc Viet Hung , Truong Thao Nguyen , Nguyen Phi Le
Published: September 2023

Due to the significant resemblance in visual appearance, pill misuse is prevalent and has become a critical issue, responsible for one-third of all deaths worldwide. Pill identification, thus, is a crucial concern that needs to be investigated thoroughly. Recently, several attempts have been made to exploit deep learning to tackle the pill identification problem. However, most published works consider only single-pill identification and fail to distinguish hard samples with identical appearances. Also, most existing pill image datasets only feature single pill images captured in carefully controlled environments under ideal lighting conditions and clean backgrounds. In this work, we are the first to tackle the multi-pill detection problem in real-world settings, aiming at localizing and identifying pills captured by users during pill intake. Moreover, we also introduce a multi-pill image dataset taken in unconstrained conditions. To handle hard samples, we propose a novel method for constructing heterogeneous a priori graphs incorporating three forms of inter-pill relationships, including co-occurrence likelihood, relative size, and visual semantic correlation. We then offer a framework for integrating a priori with pills’ visual features to enhance detection accuracy. Our experimental results have proved the robustness, reliability, and explainability of the proposed framework. Experimentally, it outperforms all detection benchmarks in terms of all evaluation metrics. Specifically, our proposed framework improves COCO mAP metrics by 9.4% over Faster R-CNN and 12.0% compared to vanilla YOLOv5. Our study opens up new opportunities for protecting patients from medication errors using an AI-based pill identification solution.
Impact of anthropometry training and feasibility of 3D imaging on anthropometry data quality among children under five years in a postmortem setting
Authors: Gupta Priya M. , Sivalogan Kasthuri , Oliech Richard , Alexander Eugene , Klein Jamie , Addo O. Yaw. , Gethi Dickson , Akelo Victor , Blau Dianna M. , Suchdev Parminder S.
Published: September 2023

Background

The Child Health and Mortality Prevention Surveillance Network (CHAMPS) identifies causes of under-5 mortality in high mortality countries.

Objective

To address challenges in postmortem nutritional assessment, we evaluated the impact of anthropometry training and the feasibility of 3D imaging on data quality within the CHAMPS Kenya site.

Design

Staff were trained using World Health Organization (WHO)-recommended manual anthropometry equipment and novel 3D imaging methods to collect postmortem measurements. Following training, 76 deceased children were measured in duplicate and were compared to measurements of 75 pre-training deceased children. Outcomes included measures of data quality (standard deviations of anthropometric indices and digit preference scores (DPS)), precision (absolute and relative technical errors of measurement, TEMs or rTEMs), and accuracy (Bland-Altman plots). WHO growth standards were used to produce anthropometric indices. Post-training surveys and in-depth interviews collected qualitative feedback on measurer experience with performing manual anthropometry and ease of using 3D imaging software.

Results

Manual anthropometry data quality improved after training, as indicated by DPS. Standard deviations of anthropometric indices exceeded limits for high data quality when using the WHO growth standards. Reliability of measurements post-training was high as indicated by rTEMs below 1.5%. 3D imaging was highly correlated with manual measurements; however, on average 3D scans overestimated length and head circumference by 1.61 cm and 2.27 cm, respectively. Site staff preferred manual anthropometry to 3D imaging, as the imaging technology required adequate lighting and additional considerations when performing the measurements.

Conclusions

Manual anthropometry was feasible and reliable postmortem in the presence of rigor mortis. 3D imaging may be an accurate alternative to manual anthropometry, but technology adjustments are needed to ensure accuracy and usability.
The semi-arid ecosystem of Asiatic Lion Landscape in Saurashtra, Gujarat: Population density, biomass and conservation of nine wild prey species
Authors: Ram Mohan , Sahu Aradhana , Srivastava Nityanand , Chaudhary Rohit , Jhala Lahar , Zala Yashpal
Published: September 2023

The present study aimed to assess the population density, structure, and population change of nine wild prey species in the semi-arid landscape of Saurashtra, Gujarat, India. A total of eight sites, representing a gradient from highly protected woodlands and grasslands to unreserved grasslands, were selected for sampling. We employed the road transect methodology under a distance sampling framework to achieve our objectives. We evaluated the realized growth rate of the Gir ungulate population through linear regression analysis. Our findings revealed that deer species exhibited higher density and biomass in woodlands compared to grasslands and coastal forests. On the other hand, antelopes showed higher density and biomass in grasslands and coastal forests compared to woodlands. The density gradient of wild prey species was influenced by various factors, including habitat structure, social organization, grouping tendencies, and topography. Over the last four decades, the population of wild prey species in Gir showed minimal changes. Our study provides a comprehensive understanding of wild prey species’ density and biomass patterns at the landscape level. The inclusion of findings from ecologically significant and unique areas, such as coastal forests, further enhances the importance of this study. The implications of this study extend beyond the conservation of wild prey species alone; they also contribute to the conservation of the large carnivore guild in the Saurashtra landscape.
The Helix-Loop-Helix motif of human EIF3A regulates translation of proliferative cellular mRNAs
Authors: Volegova Marina P. , Hermosillo Cynthia , Cate Jamie H. D.
Published: September 2023

Improper regulation of translation initiation, a vital checkpoint of protein synthesis in the cell, has been linked to a number of cancers. Overexpression of protein subunits of eukaryotic translation initiation factor 3 (eIF3) is associated with increased translation of mRNAs involved in cell proliferation. In addition to playing a major role in general translation initiation by serving as a scaffold for the assembly of translation initiation complexes, eIF3 regulates translation of specific cellular mRNAs and viral RNAs. Mutations in the N-terminal Helix-Loop-Helix (HLH) RNA-binding motif of the EIF3A subunit interfere with Hepatitis C Virus Internal Ribosome Entry Site (IRES) mediated translation initiation in vitro. Here we show that the EIF3A HLH motif controls translation of a small set of cellular transcripts enriched in oncogenic mRNAs, including MYC. We demonstrate that the HLH motif of EIF3A acts specifically on the 5′ UTR of MYC mRNA and modulates the function of EIF4A1 on select transcripts during translation initiation. In Ramos lymphoma cell lines, which are dependent on MYC overexpression, mutations in the HLH motif greatly reduce MYC expression, impede proliferation and sensitize cells to anti-cancer compounds. These results reveal the potential of the EIF3A HLH motif in eIF3 as a promising chemotherapeutic target.
Quantitative sonographic assessment of muscle thickness and fasciculations distribution is a sensitive tool for neuromuscular disorders
Authors: Abraham Alon , Fainmesser Yaara , Drory Vivian E. , Bril Vera
Published: September 2023

Introduction

Loss of muscle thickness can be demonstrated in a wide spectrum of neuromuscular disorders, while fasciculations are more frequent in amyotrophic lateral sclerosis (ALS). In the current study, we aimed to determine the sensitivity and specificity of quantitative sonographic assessment of muscle thickness and the presence of fasciculations for diagnosing various neuromuscular disorders.

Methods

The thickness and the presence of fasciculations in eight muscles were determined by sonography in patients with myopathy (22), polyneuropathy (36), ALS (91), and spinal muscular atrophy (SMA) (31) and compared to normative values determined in 65 heathy control subjects.

Results

Reduced muscle thickness in at least one relaxed muscle showed 92–100% sensitivity for diagnosing a neuromuscular disease, with a specificity of 85% for differentiating patients from heathy controls (AUC = 0.90). Subtracting distal from proximal muscle thickness may differentiate between myopathy and polyneuropathy. Fasciculations in ≥1 proximal muscle showed good diagnostic accuracy (AUC = 0.87) for diagnosing ALS.

Discussion

Sonographic assessment of muscle thickness is a sensitive tool for diagnosing a wide spectrum of neuromuscular diseases, and may facilitate diagnosis even in patients with normal strength on neurological examination, while the presence of fasciculations in proximal muscles may facilitate ALS diagnosis.
Molecular characterization of juvenile fish from the Amazon estuary using DNA barcoding approach
Authors: Lutz Ítalo , Martins Thais , Araújo Felipe , Ferreira Charles , Santana Paula , Miranda Josy , Matos Suane , Sousa Jefferson , Pereira Luciano , Bentes Bianca , da Silva Raimundo , Veneza Ivana , Sampaio Iracilda , Vallinoto Marcelo , Gomes Grazielle Evangelista
Published: September 2023

The efficiency of the DNA barcoding relies on sequencing fragment of the Cytochrome C Subunit I (COI) gene, which has been claimed as a tool to biodiversity identification from distinct groups. Accordingly, the goal of this study was to identify juvenile fish species along an estuary of Caeté River in the Brazilian Blue Amazon based on. For this purpose, we applied the DNA barcoding and discuss this approach as a tool for discrimination of species in early ontogenetic stages. A 500-bp fragment was obtained from 74 individuals, belonging to 23 species, 20 genera, 13 families and seven orders. About 70% of the 46 haplotypes revealed congruence between morphological and molecular species identification, while 8% of them failed in identification of taxa and 22% demonstrated morphological misidentification. These results proved that COI fragments were effective to diagnose fish species at early life stages, allowing identifying all samples to a species-specific status, except for some taxa whose COI sequences remain unavailable in public databases. Therefore, we recommend the incorporation of DNA barcoding to provide additional support to traditional identification, especially in morphologically controversial groups. In addition, periodic updates and comparative analyses in public COI datasets are encouraged.
Pricing quanto options with market liquidity risk
Authors: Gao Rui , Bai Yanfei
Published: September 2023

This paper investigates the pricing problem of quanto options with market liquidity risk using the Bayesian method. The increasing volatility of global financial markets has made liquidity risk a significant factor that should be taken into consideration while evaluating option prices. To address this issue, we first derive the pricing formula for quanto options with liquidity risk. Next, we construct a likelihood function to conduct posterior inference on model parameters. We then propose a numerical algorithm to conduct statistical inferences on the option prices based on the posterior distribution. This proposed method considers the impact of parameter uncertainty on option prices. Finally, we conduct a comparison between the Bayesian method and traditional estimation methods to examine their validity. Empirical results show that our proposed method is feasible for pricing and predicting quanto options with liquidity risk, particularly for parameter estimations with a small sample size.
Upper stem diameter and volume prediction strategies in the National Forest Inventory of Finland
Authors: Stenman Virpi , Kangas Annika , Holopainen Markus
Published: September 2023

In forest inventories, field data are needed for the prediction of tree volumes. However, gathering field data requires resources, such as labour, equipment, and data management operations. This means that time and budget, as well as quality, must be carefully considered when National Forest Inventory (NFI) field measurement activities are planned. Therefore, the development of cost efficient, simple, safe and reliable measurement methods and tools are of great interest. To date, upper stem diameter (d6), which provides a more reliable estimation of tree stem volume, has typically been measured with a parabolic calliper. In this study, the performance of the Criterion laser-based dendrometer was examined for d6 measurements. A total of 326 sample trees were measured multiple times with three different measurement instruments. These instruments were used to measure diameter at breast height (dbh) as well as d6 measurements. Bland-Altman plots and measurement error variances were used to determine measurement instrument reliability. For all trees, the standard deviation for the laser based dendrometer was 18.73 mm at dbh and 15.36 mm for the d6 measurements. When the performance of Criterion was analysed with reference to the mean value of repeated measurements, the standard deviation in the dbh measurements was 12.21 mm, and 8.88 mm in the d6 measurements.