bluuebunny/crossref_metadata_2025 huggingface dataset:
Burden of Disease Due to Traffic Noise in Germany
Authors: Tobollik Myriam , Hintzsche Matthias , Wothge Jördis , Myck Thomas , Plass Dietrich
Published: June 2019

Traffic noise is nearly ubiquitous and thus can affect the health of many people. Using the German noise mapping data according to the Directive 2002/49/EC of 2017 and exposure-response functions for ischemic heart disease, noise annoyance and sleep disturbance assessed by the World Health Organization’s Environmental Noise Guidelines for the European Region the burden of disease due to traffic noise is quantified. The burden of disease is expressed in disability-adjusted life years (DALYs) and its components. The highest burden was found for road traffic noise, with 75,896 DALYs when only considering moderate evidence. When including all available evidence, 176,888 DALYs can be attributable to road traffic noise. The burden due to aircraft and railway noise is lower because fewer people are exposed. Comparing the burden by health outcomes, the biggest share is due to ischemic heart disease (90%) in regard to aircraft noise, however, the lowest evidence was expressed for the association between traffic noise and ischemic heart disease. Therefore, the results should be interpreted with caution. Using alternative input parameters (e.g., exposure data) can lead to a much higher burden. Nevertheless, environmental noise is an important risk factor which leads to considerable loss of healthy life years.
Differences in Autonomy and Health-Related Quality of Life between Resilient and Non-Resilient Individuals with Mild Cognitive Impairment
Authors: Clement-Carbonell Violeta , Ferrer-Cascales Rosario , Ruiz-Robledillo Nicolás , Rubio-Aparicio María , Portilla-Tamarit Irene , Cabañero-Martínez María José
Published: July 2019

The dramatic increase in the number of older people with Mild Cognitive Impairment (MCI) entails a serious public health problem. MCI involves different degrees of dependence that has been previously related to a decrease in Health-Related Quality of Life (HRQoL), due to impairment in the performance of activities of daily living. Resilient coping, as an adaptive coping style, could reduce the associated limitations derived by the characteristic deficits of MCI, and hence improve HRQoL. The principal objective of this work was to compare the level of autonomy (measured in terms of independence in the performance of basic (ADL) and instrumental (IADL) activities of daily living), and HRQoL between resilient and non-resilient individuals with MCI. The results showed a positive relationship between resilience, autonomy, and HRQoL. Hence, resilient participants exhibited higher independence in daily living activities and better HRQoL than non-resilient individuals. Mediation analyses confirmed an indirect influence of resilience on HRQoL through the mediation effect of better performance in IADLs. These findings underline the relevance of resilience as a coping style to compensate deficits in daily living in people with MCI. The inclusion of intervention programs, oriented to the promotion of resilience coping for older adults, might increase the autonomy levels in this population, improving their HRQoL.
Urban Road Network Expansion and Its Driving Variables: A Case Study of Nanjing City
Authors: Shi Ge , Shan Jie , Ding Liang , Ye Peng , Li Yang , Jiang Nan
Published: July 2019

Developing countries such as China are undergoing rapid urban expansion and land use change. Urban expansion regulation has been a significant research topic recently, especially in Eastern China, with a high urbanization level. Among others, roads are an important spatial determinant of urban expansion and have significant influences on human activities, the environment, and socioeconomic development. Understanding the urban road network expansion pattern and its corresponding social and environmental effects is a reasonable way to optimize comprehensive urban planning and keep the city sustainable. This paper analyzes the spatiotemporal dynamics of urban road growth and uses spatial statistic models to describe its spatial patterns in rapid developing cities through a case study of Nanjing, China. A kernel density estimation model is used to describe the spatiotemporal distribution patterns of the road network. A geographically weighted regression (GWR) is applied to generate the social and environmental variance influenced by the urban road network expansion. The results reveal that the distribution of the road network shows a morphological character of two horizontal and one vertical concentration lines. From 2012 to 2016, the density of the urban road network increased significantly and developed some obvious focus centers. The development of the urban road network had a strong correlation with socioeconomic and environmental factors, which however, influenced it at different degrees in different districts. This study enhances the understanding of the effects of socio-economic and environmental factors on urban road network expansion, a significant indicator of urban expansion, in different circumstances. The study will provide useful understanding and knowledge to planning departments and other decision makers to maintain sustainable development.
Temporary Workers’ Skipping of Meals and Eating Alone in South Korea: The Korean National Health and Nutrition Examination Survey for 2013–2016
Authors: Kong Ji-Sook , Min Kyoung-Bok , Min Jin-Young
Published: July 2019

Available evidence suggests that social disadvantages are inextricably linked to unhealthy eating behaviors. Given that temporary workers face insecure employment and uncertainty in their work’s terms and conditions, issues relevant to maintaining healthy eating behavior are likely to be affected. This study investigated the association between temporary employments and, specifically, the status and frequency of meal skipping and of eating alone among temporary and permanent Korean workers. We used data from the 2013–2016 Korean National Health and Nutrition Examination Survey. A total of 5912 working people were included as the study population. We classified them as temporary workers (n = 3036) and permanent workers (n = 2876). Eating behaviors included meal skipping and eating alone. The rate and frequency of meal skipping and eating alone were higher in temporary workers. After adjustment for potential confounders, the likelihoods for temporary workers’ skipping lunch was twice as high (OR = 1.95, 95% CI 1.45–2.63) as for permanent workers. In particular, temporary workers had four-fold-increased odds (OR = 4.12, 95% CI 2.29–7.41) of eating alone three times per day relative to permanent workers. We found that temporary workers were more likely to skip meals and eat alone than were permanent workers.
Influence of Alcohol Consumption on Body Mass Gain and Liver Antioxidant Defense in Adolescent Growing Male Rats
Authors: Kołota Aleksandra , Głąbska Dominika , Oczkowski Michał , Gromadzka-Ostrowska Joanna
Published: July 2019

The World Health Organization (WHO) reported that alcohol consumption is a serious problem in adolescents. The aim of the study was to assess the influence of the time of exposure of various alcoholic beverages on body mass as well as on select parameters of liver antioxidant defense in adolescent Wistar rats. Thirty-day-old animals were divided into 12 groups (six animals in each): control and groups receiving various beverages containing 10% of alcohol (ethanol, red wine, beer), observed for two, four, and six weeks. The body weight gain and energy supply were analyzed for body mass assessment. The catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase, transferase (GST), reductase activities, total antioxidant status, and glutathione level (GSH) were analyzed, for a liver antioxidant defense assessment. Group receiving red wine was characterized by the highest alcohol intake, lowest dietary intake, and highest total energy supply (p < 0.05). However, this did not influence body weight gain (p > 0.05). Reduced diet intake in groups receiving alcohol was counterbalanced by its energy value. Therefore, the energy supply was not lower than for the control (p > 0.05). Alcohol consumption and the experiment duration influenced CAT, SOD, and GST activities and GSH level. Alcohol consumption may influence hepatic antioxidant defense in adolescent male rats, but without influence on body weight gain.
Applying the Personal and Social Responsibility Model-Based Program: Differences According to Gender between Basic Psychological Needs, Motivation, Life Satisfaction and Intention to be Physically Active
Authors: Manzano-Sánchez David , Valero-Valenzuela Alfonso , Conde-Sánchez Antonio , Chen Ming-Yao
Published: July 2019

The objective of the study was to evaluate the impact of a program based on the Teaching Personal and Social Responsibility (TPSR) on the variables of responsibility, basic psychology needs, motivation, satisfaction with life and the intention to be physically active, as well as the differences of gender. The participants were 85 students (experimental group n = 35, 17 girls and control group n = 50, 28 girls). The students of the experimental group received the TPSR for 8 months within the physical education subject. The findings indicated an improvement in the experimental group in terms of personal responsibility and in the case of female students, in basic psychological needs and intrinsic motivation. In conclusion, the TPSR program can be integrated into the physical education curriculum in order to improve the personal responsibility of students and fulfill their motivation and satisfaction of basic psychological needs.
Does Perceived Green Space Quality Matter? Linking Norwegian Adult Perspectives on Perceived Quality to Motivation and Frequency of Visits
Authors: Fongar Claudia , Aamodt Geir , Randrup Thomas B. , Solfjeld Ingjerd
Published: July 2019

Individual perceptions of green space quality are essential when a user considers engaging in activities. This national-scale study provides insights into Norwegians’ quality perceptions of municipal green space, visit frequency and motivations for engaging in different activities. We applied regression analysis to investigate how various factors affect the outcome variables, quality perceptions and visit frequency from a sample of the Norwegian adult population. Results reveal that Norwegians perceive their green spaces as having good quality, and higher quality perceptions have a positive influence on green space visits. Half of the respondents visited green spaces out of intrinsic motives in high-quality environments providing fresh air, experiences of nature and quietness. It is essential, however, to take into account that less reported activity mirrors groups of respondents who least often visit green spaces.
Cystathionine-Gamma-Lyase-Derived Hydrogen Sulfide-Regulated Substance P Modulates Liver Sieve Fenestrations in Caecal Ligation and Puncture-Induced Sepsis
Authors: Gaddam Ravinder , Chambers Stephen , Fraser Robin , Cogger Victoria , Le Couteur David , Ishii Isao , Bhatia Madhav
Published: July 2019

Cystathionine-γ-lyase (CSE) is a hydrogen sulfide (H2S)-synthesizing enzyme that promotes inflammation by upregulating H2S in sepsis. Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells (liver sieve) that undergo alteration during sepsis and H2S plays a role in this process. Substance P (SP) is encoded by the preprotachykinin A (PPTA) gene, and promotes inflammation in sepsis; however, its regulation by H2S is poorly understood. Furthermore, the interaction between H2S and SP in modulating LSEC fenestrations following sepsis remains unclear. This study aimed to investigate whether CSE/H2S regulates SP and the neurokinin-1 receptor (NK-1R) and modulates fenestrations in LSECs following caecal ligation and puncture (CLP)-induced sepsis. Here we report that the absence of either CSE or H2S protects against liver sieve defenestration and gaps formation in LSECs in sepsis by decreased SP-NK-1R signaling. Following sepsis, there is an increased expression of liver CSE and H2S synthesis, and plasma H2S levels, which were aligned with higher SP levels in the liver, lungs and plasma and NK-1R in the liver and lungs. The genetic deletion of CSE led to decreased sepsis-induced SP and NK-1R in the liver, lungs and plasma SP suggesting H2S synthesized through CSE regulates the SP-NK-1R pathway in sepsis. Further, mice deficient in the SP-encoding gene (PPTA) preserved sepsis-induced LSEC defenestration and gaps formation, as seen by maintenance of patent fenestrations and fewer gaps. In conclusion, CSE/H2S regulates SP-NK-1R and modulates LSEC fenestrations in sepsis.
Galectin 13 (PP13) Facilitates Remodeling and Structural Stabilization of Maternal Vessels during Pregnancy
Authors: Sammar Marei , Drobnjak Tijana , Mandala Maurizio , Gizurarson Sveinbjörn , Huppertz Berthold , Meiri Hamutal
Published: July 2019

Galectins regulate cell growth, proliferation, differentiation, apoptosis, signal transduction, mRNA splicing, and interactions with the extracellular matrix. Here we focus on the galectins in the reproductive system, particularly on a group of six galectins that first appears in anthropoid primates in conjunction with the evolution of highly invasive placentation and long gestation. Of these six, placental protein 13 (PP13, galectin 13) interacts with glycoproteins and glycolipids to enable successful pregnancy. PP13 is related to the development of a major obstetric syndrome, preeclampsia, a life-threatening complication of pregnancy which affects ten million pregnant women globally. Preeclampsia is characterized by hypertension, proteinuria, and organ failure, and is often accompanied by fetal loss and major newborn disabilities. PP13 facilitates the expansion of uterine arteries and veins during pregnancy in an endothelial cell-dependent manner, via the eNOS and prostaglandin signaling pathways. PP13 acts through its carbohydrate recognition domain that binds to sugar residues of extracellular and connective tissue molecules, thus inducing structural stabilization of vessel expansion. Further, decidual PP13 aggregates may serve as a decoy that induces white blood cell apoptosis, contributing to the mother’s immune tolerance to pregnancy. Lower first trimester PP13 level is one of the biomarkers to predict the subsequent risk to develop preeclampsia, while its molecular mutations/polymorphisms that are associated with reduced PP13 expression are accompanied by higher rates of preeclampsia We propose a targeted PP13 replenishing therapy to fight preeclampsia in carriers of these mutations.
Endothelial Dysfunction in Chronic Heart Failure: Assessment, Findings, Significance, and Potential Therapeutic Targets
Authors: Alem Manal M.
Published: July 2019

Chronic heart failure (CHF) is a complex syndrome that results from structural and functional disturbances that affect the ability of the heart to supply oxygen to tissues. It largely affects and reduces the patient’s quality of life, socio-economic status, and imposes great costs on health care systems worldwide. Endothelial dysfunction (ED) is a newly discovered phenomenon that contributes greatly to the pathophysiology of numerous cardiovascular conditions and commonly co-exists with chronic heart failure. However, the literature lacks clarity as to which heart failure patients might be affected, its significance in CHF patients, and its reversibility with pharmacological and non-pharmacological means. This review will emphasize all these points and summarize them for future researchers interested in vascular pathophysiology in this particular patient population. It will help to direct future studies for better characterization of these two phenomena for the potential discovery of therapeutic targets that might reduce future morbidity and mortality in this “at risk” population.
The Insulin-Like Growth Factor 2 mRNA Binding Protein IMP2/IGF2BP2 is Overexpressed and Correlates with Poor Survival in Pancreatic Cancer
Authors: Dahlem Charlotte , Barghash Ahmad , Puchas Philip , Haybaeck Johannes , Kessler Sonja M.
Published: July 2019

The insulin-like growth factor 2 (IGF2) mRNA binding protein IMP2 (IGF2BP2) is an oncogenic protein known to be overexpressed in different tumor types. Pancreatic cancer is a very lethal cancer that requires early diagnosis and new treatment options. The aim of our study was to investigate the role of IMP2 in the initiation and progression of pancreatic ductal adenocarcinoma (PDAC). IMP2 was significantly overexpressed in a human precursor (PanIN) lesions suggesting IMP2 as a marker for early stages of PDAC. In a PDAC cohort of matched normal and tumor samples IMP2 showed overexpression in tumor tissues compared with normal pancreatic tissue. Strict correlation analysis (threshold R2 > 0.75) revealed 22 genes highly positively and 9 genes highly negatively correlating with IMP2. Besides genes involved in the inhibition of apoptosis (Bcl-XL), especially factors involved in ubiquitination were strongly correlated with IMP2 expression: SMURF1 and FBXO45. Moreover, protein kinase C (PKC) signaling pathway was distinctly affected: DXS1179E encoding PKC iota, PKC substrate PLEK2, and inositol triphosphate receptor IP3R3 were positively correlated with IMP2 expression. Besides tumor initiation, IMP2 also seemed to have an impact on tumor progression. TGF-β treatment of Panc-1 pancreatic cancer cells to induce epithelial-mesenchymal transition (EMT) was accompanied by increased IMP2 expression. EMT is important for cancer cells to gain migratory and invasive potential, which is essential for metastasis. Concordantly, circulating tumor cells showed higher IMP2 levels as compared with normal tissue from tumor origin and with normal hematological cells. Accordingly, IMP2 protein levels correlated with poor survival. In conclusion, as IMP2 seems to promote tumor progression of PDAC, it might be an interesting diagnostic and prognostic marker as well as a novel target for the treatment of PDAC.
RETRACTED: Beneficial Role of HO-1-SIRT1 Axis in Attenuating Angiotensin II-Induced Adipocyte Dysfunction
Authors: Lakhani Hari , Zehra Mishghan , Pillai Sneha , Puri Nitin , Shapiro Joseph , Abraham Nader , Sodhi Komal
Published: July 2019

Background: Angiotensin II (Ang II), released by the renin–angiotensin–aldosterone system (RAAS), contributes to the modulatory role of the RAAS in adipose tissue dysfunction. Investigators have shown that inhibition of AngII improved adipose tissue function and insulin resistance in mice with metabolic syndrome. Heme Oxygenase-1 (HO-1), a potent antioxidant, has been demonstrated to improve oxidative stress and adipocyte phenotype. Molecular effects of high oxidative stress include suppression of sirtuin-1 (SIRT1), which is amenable to redox manipulations. The mechanisms involved, however, in these metabolic effects of the RAAS remain incompletely understood. Hypothesis: We hypothesize that AngII-induced oxidative stress has the potential to suppress adipocyte SIRT1 via down regulation of HO-1. This effect of AngII will, in turn, upregulate mineralocorticoid receptor (MR). The induction of HO-1 will rescue SIRT1, hence improving oxidative stress and adipocyte phenotype. Methods and Results: We examined the effect of AngII on lipid accumulation, oxidative stress, and inflammatory cytokines in mouse pre-adipocytes in the presence and absence of cobalt protoporphyrin (CoPP), HO-1 inducer, tin mesoporphyrin (SnMP), and HO-1 inhibitor. Our results show that treatment of mouse pre-adipocytes with AngII increased lipid accumulation, superoxide levels, inflammatory cytokine levels, interleukin-6 (IL-6) and tumor necrosis factor α (TNFα), and adiponectin levels. This effect was attenuated by HO-1 induction, which was further reversed by SnMP, suggesting HO-1 mediated improvement in adipocyte phenotype. AngII-treated pre-adipocytes also showed upregulated levels of MR and suppressed SIRT1 that was rescued by HO-1. Subsequent treatment with CoPP and SIRT1 siRNA in mouse pre-adipocytes increased lipid accumulation and fatty acid synthase (FAS) levels, suggesting that beneficial effects of HO-1 are mediated via SIRT1. Conclusion: Our study demonstrates for the first time that HO-1 has the ability to restore cellular redox, rescue SIRT1, and prevent AngII-induced impaired effects on adipocytes and the systemic metabolic profile.
Basic Concepts on the Role of Nuclear Factor Erythroid-Derived 2-Like 2 (Nrf2) in Age-Related Diseases
Authors: Francisqueti-Ferron Fabiane Valentini , Ferron Artur Junio Togneri , Garcia Jéssica Leite , Silva Carol Cristina Vágula de Almeida , Costa Mariane Róvero , Gregolin Cristina Schmitt , Moreto Fernando , Ferreira Ana Lúcia A. , Minatel Igor Otávio , Correa Camila Renata
Published: July 2019

The transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) is one of the most important oxidative stress regulator in the human body. Once Nrf2 regulates the expression of a large number of cytoprotective genes, it plays a crucial role in the prevention of several diseases, including age-related disorders. However, the involvement of Nrf2 on these conditions is complex and needs to be clarified. Here, a brief compilation of the Nrf2 enrollment in the pathophysiology of the most common age-related diseases and bring insights for future research on the Nrf2 pathway is described. This review shows a controversial response of this transcriptional factor on the presented diseases. This reinforces the necessity of more studies to investigate modulation strategies for Nrf2, making it a possible therapeutic target in the treatment of age-related disorders.
Recent Topics on The Mechanisms of Immunosuppressive Therapy-Related Neurotoxicities
Authors: Zhang Wei , Egashira Nobuaki , Masuda Satohiro
Published: July 2019

Although transplantation procedures have been developed for patients with end-stage hepatic insufficiency or other diseases, allograft rejection still threatens patient health and lifespan. Over the last few decades, the emergence of immunosuppressive agents such as calcineurin inhibitors (CNIs) and mammalian target of rapamycin (mTOR) inhibitors have strikingly increased graft survival. Unfortunately, immunosuppressive agent-related neurotoxicity commonly occurs in clinical practice, with the majority of neurotoxicity cases caused by CNIs. The possible mechanisms through which CNIs cause neurotoxicity include increasing the permeability or injury of the blood–brain barrier, alterations of mitochondrial function, and alterations in the electrophysiological state. Other immunosuppressants can also induce neuropsychiatric complications. For example, mTOR inhibitors induce seizures, mycophenolate mofetil induces depression and headaches, methotrexate affects the central nervous system, the mouse monoclonal immunoglobulin G2 antibody (used against the cluster of differentiation 3) also induces headaches, and patients using corticosteroids usually experience cognitive alteration. Therapeutic drug monitoring, individual therapy based on pharmacogenetics, and early recognition of symptoms help reduce neurotoxic events considerably. Once neurotoxicity occurs, a reduction in the drug dosage, switching to other immunosuppressants, combination therapy with drugs used to treat the neuropsychiatric manifestation, or blood purification therapy have proven to be effective against neurotoxicity. In this review, we summarize recent topics on the mechanisms of immunosuppressive drug-related neurotoxicity. In addition, information about the neuroprotective effects of several immunosuppressants is also discussed.
Selective Anticancer Therapy Using Pro-Oxidant Drug-Loaded Chitosan–Fucoidan Nanoparticles
Authors: Choi Dae Gun , Venkatesan Jayachandran , Shim Min Suk
Published: July 2019

Pro-oxidant therapy exploiting pro-oxidant drugs that can trigger cytotoxic oxidative stress in cancer cells has emerged as an innovative strategy for cancer-specific therapy. Piperlongumine (PL) has gained great interest as a novel pro-oxidant agent, because it has an ability to trigger cancer-specific apoptosis through the increase of oxidative stress in cancer cells. However, the use of PL is limited in the clinic because of its hydrophobic nature. In this study, chitosan- and fucoidan-based nanoparticles were prepared for the effective intracellular delivery of PL into cancer cells. Chitosan and fucoidan formed nanoparticles by ionic gelation. The chitosan- and fucoidan-based nanoparticles (CS–F NPs) effectively encapsulated PL, and increased its water solubility and bioavailability. CS–F NPs showed very low cytotoxicity in human prostate cancer cells, demonstrating its high potential for in vivo applications. The PL-loaded chitosan–fucoidan nanoparticles (PL-CS–F NPs) efficiently killed human prostate cancer cells via PL-induced intracellular reactive oxygen species (ROS) generation. This study demonstrates that CS–F NPs are promising natural polymer-based drug carriers for safe and effective PL delivery.
Single Mild Traumatic Brain Injury Induces Persistent Disruption of the Blood-Brain Barrier, Neuroinflammation and Cognitive Decline in Hypertensive Rats
Authors: Szarka Nikolett , Toth Luca , Czigler Andras , Kellermayer Zoltan , Ungvari Zoltan , Amrein Krisztina , Czeiter Endre , Bali Zsolt Kristof , Tadepalli Sai Ambika , Wahr Matyas , Hernadi Istvan , Koller Akos , Buki Andras , Toth Peter
Published: July 2019

Traumatic brain injury (TBI) induces blood-brain barrier (BBB) disruption, which contributes to secondary injury of brain tissue and development of chronic cognitive decline. However, single mild (m)TBI, the most frequent form of brain trauma disrupts the BBB only transiently. We hypothesized, that co-morbid conditions exacerbate persistent BBB disruption after mTBI leading to long term cognitive dysfunction. Since hypertension is the most important cerebrovascular risk factor in populations prone to mild brain trauma, we induced mTBI in normotensive Wistar and spontaneously hypertensive rats (SHR) and we assessed BBB permeability, extravasation of blood-borne substances, neuroinflammation and cognitive function two weeks after trauma. We found that mTBI induced a significant BBB disruption two weeks after trauma in SHRs but not in normotensive Wistar rats, which was associated with a significant accumulation of fibrin and increased neuronal expression of inflammatory cytokines TNFα, IL-1β and IL-6 in the cortex and hippocampus. SHRs showed impaired learning and memory two weeks after mild TBI, whereas cognitive function of normotensive Wistar rats remained intact. Future studies should establish the mechanisms through which hypertension and mild TBI interact to promote persistent BBB disruption, neuroinflammation and cognitive decline to provide neuroprotection and improve cognitive function in patients with mTBI.
Maternal and Post-Weaning High-Fat Diets Produce Distinct DNA Methylation Patterns in Hepatic Metabolic Pathways within Specific Genomic Contexts
Authors: Moody Laura , Wang Huan , Jung Paul M. , Chen Hong , Pan Yuan-Xiang
Published: July 2019

Calorie-dense high-fat diets (HF) are associated with detrimental health outcomes, including obesity, cardiovascular disease, and diabetes. Both pre- and post-natal HF diets have been hypothesized to negatively impact long-term metabolic health via epigenetic mechanisms. To understand how the timing of HF diet intake impacts DNA methylation and metabolism, male Sprague–Dawley rats were exposed to either maternal HF (MHF) or post-weaning HF diet (PHF). At post-natal week 12, PHF rats had similar body weights but greater hepatic lipid accumulation compared to the MHF rats. Genome-wide DNA methylation was evaluated, and analysis revealed 1744 differentially methylation regions (DMRs) between the groups with the majority of the DMR located outside of gene-coding regions. Within differentially methylated genes (DMGs), intragenic DNA methylation closer to the transcription start site was associated with lower gene expression, whereas DNA methylation further downstream was positively correlated with gene expression. The insulin and phosphatidylinositol (PI) signaling pathways were enriched with 25 DMRs that were associated with 20 DMGs, including PI3 kinase (Pi3k), pyruvate kinase (Pklr), and phosphodiesterase 3 (Pde3). Together, these results suggest that the timing of HF diet intake determines DNA methylation and gene expression patterns in hepatic metabolic pathways that target specific genomic contexts.
Extracellular Vesicles Enhance Multiple Myeloma Metastatic Dissemination
Authors: Colombo Michela , Giannandrea Domenica , Lesma Elena , Basile Andrea , Chiaramonte Raffaella
Published: July 2019

Extracellular vesicles (EVs) represent a heterogeneous group of membranous structures shed by all kinds of cell types, which are released into the surrounding microenvironment or spread to distant sites through the circulation. Therefore, EVs are key mediators of the communication between tumor cells and the surrounding microenvironment or the distant premetastatic niche due to their ability to transport lipids, transcription factors, mRNAs, non-coding regulatory RNAs, and proteins. Multiple myeloma (MM) is a hematological neoplasm that mostly relies on the bone marrow (BM). The BM represents a highly supportive niche for myeloma establishment and diffusion during the formation of distant bone lesions typical of this disease. This review represents a survey of the most recent evidence published on the role played by EVs in supporting MM cells during the multiple steps of metastasis, including travel and uptake at distant premetastatic niches, MM cell engraftment as micrometastasis, and expansion to macrometastasis thanks to EV-induced angiogenesis, release of angiocrine factors, activation of osteolytic activity, and mesenchymal cell support. Finally, we illustrate the first evidence concerning the dual effect of MM-EVs in promoting both anti-tumor immunity and MM immune escape, and the possible modulation operated by pharmacological treatments.
Big Data Analytics and Firm Performance: A Systematic Review
Authors: Maroufkhani Parisa , Wagner Ralf , Wan Ismail Wan Khairuzzaman , Baroto Mas Bambang , Nourani Mohammad
Published: July 2019

The literature on big data analytics and firm performance is still fragmented and lacking in attempts to integrate the current studies’ results. This study aims to provide a systematic review of contributions related to big data analytics and firm performance. The authors assess papers listed in the Web of Science index. This study identifies the factors that may influence the adoption of big data analytics in various parts of an organization and categorizes the diverse types of performance that big data analytics can address. Directions for future research are developed from the results. This systematic review proposes to create avenues for both conceptual and empirical research streams by emphasizing the importance of big data analytics in improving firm performance. In addition, this review offers both scholars and practitioners an increased understanding of the link between big data analytics and firm performance.
Synthesis and Characterization of Chitosan/Reduced Graphene Oxide Hybrid Composites
Authors: Kosowska Karolina , Domalik-Pyzik Patrycja , Krok-Borkowicz Małgorzata , Chłopek Jan
Published: June 2019

Graphene family materials (GFM) are currently considered to be one of the most interesting nanomaterials with a wide range of application. They can also be used as modifiers of polymer matrices to develop composite materials with favorable properties. In this study, hybrid nanocomposites based on chitosan (CS) and reduced graphene oxide (rGO) were fabricated for potential use in bone tissue engineering. CS/rGO hydrogels were prepared by simultaneous reduction and composite formation in acetic acid or lactic acid and crosslinked with a natural agent—tannic acid (TAc). A broad spectrum of research methods was applied in order to thoroughly characterize both the components and the composite systems, i.e., X-ray Photoelectron Spectroscopy, X-ray Diffractometry, Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy, Scanning Electron Microscopy, ninhydrin assay, mechanical testing, in vitro degradation and bioactivity study, wettability, and, finally, cytocompatibility. The composites formed through the self-assembly of CS chains and exfoliated rGO sheets. Obtained results allowed also to conclude that the type of solvent used impacts the polymer structure and its ability to interact with rGO sheets and the mechanical properties of the composites. Both rGO and TAc acted as crosslinkers of the polymer chains. This study shows that the developed materials demonstrate the potential for use in bone tissue engineering. The next step should be their detailed biological examinations.
The Effect of Ultraviolet Photofunctionalization on a Titanium Dental Implant with Machined Surface: An In Vitro and In Vivo Study
Authors: Lee Jun-Beom , Jo Ye-Hyeon , Choi Jung-Yoo , Seol Yang-Jo , Lee Yong-Moo , Ku Young , Rhyu In-Chul , Yeo In-Sung Luke
Published: June 2019

Ultraviolet (UV) photofunctionalization has been suggested as an effective method to enhance the osseointegration of titanium surface. In this study, machined surface treated with UV light (M + UV) was compared to sandblasted, large-grit, acid-etched (SLA) surface through in vitro and in vivo studies. Groups of titanium specimens were defined as machined (M), SLA, and M + UV for the disc type, and M + UV and SLA for the implant. The discs and implants were assessed using scanning electron microscopy, confocal laser scanning microscopy, electron spectroscopy for chemical analysis, and the contact angle. Additionally, we evaluated the cell attachment, proliferation assay, and real-time polymerase chain reaction for the MC3T3-E1 cells. In a rabbit tibia model, the implants were examined to evaluate the bone-to-implant contact ratio and the bone area. In the M + UV group, we observed the lower amount of carbon, a 0°-degree contact angle, and enhanced osteogenic cell activities (p < 0.05). The histomorphometric analysis showed that a higher bone-to-implant contact ratio was found in the M + UV implant at 10 days (p < 0.05). In conclusion, the UV photofunctionalization of a Ti dental implant with M surface attained earlier osseointegration than SLA.
Anti-Biofouling and Desalination Properties of Thin Film Composite Reverse Osmosis Membranes Modified with Copper and Iron Nanoparticles
Authors: Armendariz Ontiveros M. , Quintero Y. , Llanquilef A. , Morel M. , Argentel Martínez L. , García García A. , Garcia A.
Published: June 2019

The anti-biofouling and desalination properties of thin film composite reverse osmosis membranes (TFC-RO), modified by the incorporation of copper and iron nanoparticles, were compared. Nanoparticles of metallic copper (CuNPs) and an iron crystalline phase mix (Fe and Fe2O3, FeNPs) were obtained by oxide-reduction-precipitation and reduction reactions, respectively, and characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM) techniques. Modified membranes (PA+0.25Cu-PSL and PA+0.25Fe-PSL) were obtained by incorporating these nanoparticles during the interfacial polymerization process (PI). These membranes were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), and contact angle measurements. Bactericidal tests by a Colony Forming Unit (CFU) were performed using Escherichia coli, and anti-adhesion properties were confirmed by fluorescence microscopy estimating the percentage of live/dead cells. The permeate flow and rejection of salts was evaluated using a crossflow cell. An increase of the membrane’s roughness on the modified membrane was observed, influencing the desalination performance more strongly in the presence of the FeNPs with respect to the CuNPs. Moreover, a significant bactericidal and anti-adhesion effect was obtained in presence of both modifications with respect to the pristine membrane. An important decrease in CFU in the presence of modified membranes of around 98% in both modifications was observed. However, the anti-adhesion percentage and reduction of live/dead cells were higher in the presence of the copper-modified membrane in comparison to the iron-modified membrane. These facts were attributed to the differences in antimicrobial action mechanism of these types of nanoparticles. In conclusion, TFC-RO membranes modified by the incorporation of CuNPs during PI represent one alternative material to attend to the biofouling impact in the desalination process.
Stability Research Considering Non-Linear Change in the Machining of Titanium Thin-Walled Parts
Authors: Gao Haining , Liu Xianli
Published: June 2019

Aiming to solve the problem whereby the damping process effect is significant and difficult to measure during low-speed machining of titanium alloy thin-walled parts, the ploughing coefficient of the flank face is obtained based on the frequency-domain decomposition (FDD) of the measured vibration signal and the energy balance principle, and then the process-damping prediction model is obtained. Aiming to solve the problem of non-linear change of dynamic characteristics of a workpiece caused by the material removal effect in the machining of titanium alloy thin-walled parts, a prediction model of dynamic characteristics of a workpiece is established based on the structural dynamic modification method. Meanwhile, the effect of material removal on the process-damping coefficient is studied, and the internal relationship between the process-damping coefficient and the dynamic characteristics of the workpiece is revealed. The stability lobe diagram is obtained by the full discretization in the titanium alloy milling process. The correctness of the model and stability prediction is verified by experiments under different working conditions. It is found that the coupling characteristics of process-damping and workpiece dynamic characteristics control the stability of the milling process. The research results can provide theoretical support for accurate characterization and process optimization of titanium alloy thin-walled workpiece milling.
Preliminary Investigation on Auto-Thermal Extrusion of Ground Tire Rubber
Authors: Zedler Łukasz , Kowalkowska-Zedler Daria , Vahabi Henri , Saeb Mohammad Reza , Colom Xavier , Cañavate Javier , Wang Shifeng , Formela Krzysztof
Published: June 2019

Ground tire rubber (GTR) was processed using an auto-thermal extrusion as a prerequisite to green reclaiming of waste rubbers. The reclaimed GTR underwent a series of tests: thermogravimetric analysis combined with Fourier-transform infrared spectroscopy (TGA-FTIR), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and static headspace and gas chromatography-mass spectrometry (SHS-GC-MS) in order to evaluate the impact of barrel heating conditions (with/without external barrel heating) on the reclaiming process of GTR. Moreover, samples were cured to assess the impact of reclaiming heating conditions on curing characteristics and physico-mechanical properties. Detailed analysis of the results indicated that the application of auto-thermal extrusion is a promising approach for the sustainable development of reclaiming technologies.
Stress Corrosion Cracking Behavior of Alloy 600 Coupled to Magnetite under High-Temperature Caustic Conditions
Authors: Song Geun Dong , Han Jeoh , Jeon Soon-Hyeok , Hur Do Haeng
Published: June 2019

This study aims to investigate and explain the magnetite-accelerated stress corrosion cracking phenomenon of Alloy 600 under caustic conditions, based on the electrochemical behavior. After the SCC test that lasted for 300 h, no cracks were observed in any of the magnetite-free specimens, whereas cracks with a depth of 150 to 280 μm were generated in all the magnetite-deposited specimens. Furthermore, the electrochemical behavior of magnetite and Alloy 600 demonstrated that Alloy 600 behaved as an anode in the coupling system with magnetite. In this coupling system, the electrochemical potential of Alloy 600 can be shifted into the range potentially susceptible to stress corrosion cracking.
The Influence of Steel Fiber Tensile Strengths and Aspect Ratios on the Fracture Properties of High-Strength Concrete
Authors: Choi Won-Chang , Jung Kwon-Young , Jang Seok-Joon , Yun Hyun-Do
Published: July 2019

Steel fiber embedded in concrete serves to reduce crack development and prevent crack growth at the macroscopic level of the concrete matrix. Steel fiber-reinforced concrete (SFRC) with high compressive concrete strength is affected primarily by the dimensions, shape, content, aspect ratio, and tensile strength of the embedded steel fiber. In this study, double-ended hook steel fiber was used in SFRC with a concrete compressive strength of 80 MPa. This fiber was used for the study variables with two aspect ratios (64, 80) and tensile strength values up to 1600 MPa. The flexural performance of the SFRC specimens was evaluated using crack mouth open displacement tests, and the test results were compared with code provisions. A modified reinforcement index was also used to quantify the flexural performance based on comparisons with fracture energy.
Non-Isothermal Oxidation Behaviors and Mechanisms of Ti-Al Intermetallic Compounds
Authors: Ouyang Peixuan , Mi Guangbao , Li Peijie , He Liangju , Cao Jingxia , Huang Xu
Published: July 2019

Non-isothermal oxidation is one of the important issues for the safe application of Ti-Al alloys, so this study aimed to illustrate the non-isothermal oxidation behaviors and the corresponding mechanisms of a TiAl-based alloy in comparison with a Ti3Al-based alloy. The non-isothermal oxidation behaviors of Ti-46Al-2Cr-5Nb and Ti-24Al-15Nb-1.5Mo alloys in pure oxygen were comparatively investigated with a thermogravimetry-differential scanning calorimetry (TGA/DSC) simultaneous thermal analyzer heating from room temperature to 1450 °C with a heating rate of 40 °C/min. When the temperature rose above 1280 °C, the oxidation rate of the Ti-46Al-2Cr-5Nb alloy sharply increased and exceeded that of the Ti-24Al-15Nb-1.5Mo alloy owing to the occurrence of internal oxidation. When the temperature was higher than 1350 °C, the oxidation rate of the Ti-46Al-2Cr-5Nb alloy decreased obviously due to the generation of an oxygen-barrier β-Al2TiO5-rich layer by a chemical reaction between Al2O3 and TiO2 in the oxide scale. Based on Wagner’s theory of internal oxidation, the reason for the occurrence of internal oxidation in the Ti-46Al-2Cr-5Nb alloy is the formation of the α phase in the subsurface, while no internal oxidation occurred in the Ti-24Al-15Nb-1.5Mo alloy due to the existence of the β phase in the subsurface with the enrichment of Nb and Mo.
Remineralization of Artificial Dentin Caries Using Dentin and Enamel Matrix Proteins
Authors: Bächli Katja , Schmidlin Patrick R. , Wegehaupt Florian , Paqué Frank , Ramenzoni Liza , Botter Sander
Published: July 2019

To assess the remineralizing potential of dentin matrix proteins and enamel matrix derivatives (DMPs and EMDs) after application on artificially induced dentin lesions, given the hypothesis that these materials increase the mineral uptake, binding, and mineralization. Forty-eight caries-free human premolars were used. Teeth were cut, polished, and embedded, leaving an open window on the root surface, of which one-third was covered with a flowable composite to preserve the healthy untreated dentin. Then, samples were demineralized in Buskes solution for 33 days. A micro-CT scan prior to treatment was performed. Next, the samples were randomly allocated into four groups: (A) An untreated negative control (CON), (B) application of porcine dentin matrix proteins (DMP), (C) treatment with enamel matrix derivatives (EMD, Emdogain, Straumann), and (D) amine fluoride application (AMF, Elmex fluid, GABA). All samples were placed in artificial saliva for 21 days. A second micro-CT scan was performed, after which the change in gray scaling within a defined region of interest (0.25 mm3) was analyzed. ANCOVA was applied to discover statistical differences between the different treatments. Both, treatment with AMF; (P = 0.011 versus CON) as well as with DMP (P = 0.043 versus CON) yielded a statistically significant difference compared to the control treatment. EMD treatment was not found to differ (P > 0.05). Mainly the top layer of the defects showed clear signs of remineralization, which was also evident in CON. This study was able to visually confirm the remineralization potential of demineralized dentin especially after DMP application, which, however, did not outperform AMF. Based on this, additional studies combining proteins and fluorides are now warranted and ongoing.
Bioinspired Materials: From Living Systems to New Concepts in Materials Chemistry
Authors: Böhm Corinna F. , Harris Joe , Schodder Philipp I. , Wolf Stephan E.
Published: July 2019

Nature successfully employs inorganic solid-state materials (i.e., biominerals) and hierarchical composites as sensing elements, weapons, tools, and shelters. Optimized over hundreds of millions of years under evolutionary pressure, these materials are exceptionally well adapted to the specifications of the functions that they perform. As such, they serve today as an extensive library of engineering solutions. Key to their design is the interplay between components across length scales. This hierarchical design—a hallmark of biogenic materials—creates emergent functionality not present in the individual constituents and, moreover, confers a distinctly increased functional density, i.e., less material is needed to provide the same performance. The latter aspect is of special importance today, as climate change drives the need for the sustainable and energy-efficient production of materials. Made from mundane materials, these bioceramics act as blueprints for new concepts in the synthesis and morphosynthesis of multifunctional hierarchical materials under mild conditions. In this review, which also may serve as an introductory guide for those entering this field, we demonstrate how the pursuit of studying biomineralization transforms and enlarges our view on solid-state material design and synthesis, and how bioinspiration may allow us to overcome both conceptual and technical boundaries.
F

-Metric, F-Contraction and Common Fixed-Point Theorems with Applications
Authors: Asif Awais , Nazam Muhammad , Arshad Muhammad , Kim Sang Og
Published: July 2019

In this paper, we noticed that the existence of fixed points of F-contractions, in F -metric space, can be ensured without the third condition (F3) imposed on the Wardowski function F : ( 0 , ∞ ) → R . We obtain fixed points as well as common fixed-point results for Reich-type F-contractions for both single and set-valued mappings in F -metric spaces. To show the usability of our results, we present two examples. Also, an application to functional equations is presented. The application shows the role of fixed-point theorems in dynamic programming, which is widely used in computer programming and optimization. Our results extend and generalize the previous results in the existing literature.