bluuebunny/crossref_metadata_2025 huggingface dataset:
HO-1 Modulates Aerobic Glycolysis through LDH in Prostate Cancer Cells
Authors: Cascardo Florencia , Anselmino Nicolás , Páez Alejandra , Labanca Estefanía , Sanchis Pablo , Antico-Arciuch Valeria , Navone Nora , Gueron Geraldine , Vázquez Elba , Cotignola Javier
Published: June 2021

Prostate cancer (PCa) is the second most diagnosed malignancy and the fifth leading cause of cancer associated death in men worldwide. Dysregulation of cellular energetics has become a hallmark of cancer, evidenced by numerous connections between signaling pathways that include oncoproteins and key metabolic enzymes. We previously showed that heme oxygenase 1 (HO-1), a cellular homeostatic regulator counteracting oxidative and inflammatory damage, exhibits anti-tumoral activity in PCa cells, inhibiting cell proliferation, migration, tumor growth and angiogenesis. The aim of this study was to assess the role of HO-1 on the metabolic signature of PCa. After HO-1 pharmacological induction with hemin, PC3 and C4-2B cells exhibited a significantly impaired cellular metabolic rate, reflected by glucose uptake, ATP production, lactate dehydrogenase (LDH) activity and extracellular lactate levels. Further, we undertook a bioinformatics approach to assess the clinical significance of LDHA, LDHB and HMOX1 in PCa, identifying that high LDHA or low LDHB expression was associated with reduced relapse free survival (RFS). Interestingly, the shortest RFS was observed for PCa patients with low HMOX1 and high LDHA, while an improved prognosis was observed for those with high HMOX1 and LDHB. Thus, HO-1 induction causes a shift in the cellular metabolic profile of PCa, leading to a less aggressive phenotype of the disease.
Soft Matter Sample Environments for Time-Resolved Small Angle Neutron Scattering Experiments: A Review
Authors: Urban Volker S. , Heller William T. , Katsaras John , Bras Wim
Published: June 2021

With the promise of new, more powerful neutron sources in the future, the possibilities for time-resolved neutron scattering experiments will improve and are bound to gain in interest. While there is already a large body of work on the accurate control of temperature, pressure, and magnetic fields for static experiments, this field is less well developed for time-resolved experiments on soft condensed matter and biomaterials. We present here an overview of different sample environments and technique combinations that have been developed so far and which might inspire further developments so that one can take full advantage of both the existing facilities as well as the possibilities that future high intensity neutron sources will offer.
Halloysite and Laponite Hybrid Pigments Synthesis with Copper Chlorophyll
Authors: Micó-Vicent Bàrbara , Perales Romero Esther , Jordán-Núñez Jorge , Viqueira Valentín
Published: June 2021

Sustainable and green materials have been studied in dye and pigment productions to reduce their environment impacts from being produced and applied. Although natural dyes are an excellent choice to move from agrowaste, some improvements must be made before they are applied given their poor fastness. One way of improving natural dye properties is their adsorption into nanoclay structures to give hybrid pigments. This work used tubular halloysite and laminar laponite to adsorb and stabilize natural copper chlorophyll. With a statistical design of experiments, we observed interactions between synthesis factors, such as pH, ionic strength, and surfactant or silane modification. Cool hybrid pigments with high TSR (%) values and a wide color range were obtained by using dispersions with only distilled water at room temperature. Successful chlorophyll adsorption on both nanoclay surfaces took place by XRD and DTA analyses. The maximum natural dye absorption for both nanoclay types took place under acid conditions, pH 3–4, and in the presence of mordant. The TSR (%) improved by the silane pH interaction, and halloysite hybrid pigments obtained higher TSR values than the laponite ones. Finally, a wide chromatic green color range was obtained with the surfactant modification in both nanoclays, and the color fastening was also improved in the hybrid pigments application. The samples generated with 10% of hybrid pigments from both nanoclays and an Epoxy bioresin, show higher colorfastness than the sample with the natural chlorophyll, due to the nanoclays–dye interaction and protection.
Terahertz Photoconductive Antenna Based on a Topological Insulator Nanofilm
Authors: Kuznetsov Kirill A. , Safronenkov Daniil A. , Kuznetsov Petr I. , Kitaeva Galiya Kh.
Published: June 2021

In this study, the efficient generation of terahertz radiation by a dipole photoconductive antenna, based on a thin island film of a topological insulator, was experimentally demonstrated. The performance of the Bi1.9Sb0.1Te2Se antenna was shown to be no worse than those of a semiconductor photoconductive antenna, which is an order of magnitude thicker. The current–voltage characteristics were studied for the photo and dark currents in Bi1.9Sb0.1Te2Se. The possible mechanisms for generating terahertz waves were analyzed by comparing the characteristics of terahertz radiation of an electrically biased and unbiased topological insulator.
Transcription at a Distance in the Budding Yeast Saccharomyces cerevisiae
Authors: Spiegel JerryAnna , Arnone James T.
Published: June 2021

Proper transcriptional regulation depends on the collaboration of multiple layers of control simultaneously. Cells tightly balance cellular resources and integrate various signaling inputs to maintain homeostasis during growth, development and stressors, among other signals. Many eukaryotes, including the budding yeast Saccharomyces cerevisiae, exhibit a non-random distribution of functionally related genes throughout their genomes. This arrangement coordinates the transcription of genes that are found in clusters, and can occur over long distances. In this work, we review the current literature pertaining to gene regulation at a distance in budding yeast.
Coconut Fibre and Sawdust as Green Building Materials: A Laboratory Assessment on Physical and Mechanical Properties of Particleboards
Authors: Tawasil Dg Normaswanna binti , Aminudin Eeydzah , Abdul Shukor Lim Nor Hasanah , Nik Soh Nik Mohd Zaini , Leng Pau Chung , Ling Gabriel Hoh Teck , Ahmad Mohd Hamdan
Published: June 2021

This paper evaluates, via a laboratory assessment, the physical properties (BS EN 323:1993, BS EN 324) and mechanical performance (BS EN 310:1993) of hybrid particleboards using agricultural wastes, namely coconut fibre and sawdust. The process begins with the preparation of the materials where they are sieved and retained with the 5-mm sieve and then oven-dried. The hybrid particleboard mixed with the addition of resin (urea formaldehyde) was sprayed and hot pressed. The hot press temperature was set at 180 °C, with the resin content of 8 wt.% and the design density of 650 kg/m3 used in producing the particleboard. The percentage/ratio of the composition of sawdust (SD) to coconut fibre (CF) varied ranging from 100SD:0CF to 70SD:30CF, 50SD:50CF, 30SD:70CF, and 0SD:100CF. Meanwhile, as for the thickness of the boards, it was categorised into three groups which are 16 mm, 20 mm, and 32 mm. The particleboards were conditioned to the room temperature for seven days before being tested for physical properties and mechanical performances. The results show that the most optimum composition of sawdust to coconut fibre is 0% sawdust to 100% coconut fibre (0SD:100CF) and the optimum thickness is 20 mm, where its density is 761.99 kg/m3, swelling thickness is 11.98%, and water absorption at 37.64%. With the modulus of elasticity of 1510 N/mm2, the modulus of rupture of 17.8 N/mm2, and the internal bonding of 1.08 N/mm2, they satisfied the universal standard of Particleboard Type P3 of BS EN 312:2010.
Recent Advances in Vertically Aligned Nanocomposites with Tunable Optical Anisotropy: Fundamentals and Beyond
Authors: Wang Xuejing , Wang Haiyan
Published: June 2021

Developing reliable and tunable metamaterials is fundamental to next-generation optical-based nanodevices and computing schemes. In this review, an overview of recent progress made with a unique group of ceramic-based functional nanocomposites, i.e., vertically aligned nanocomposites (VANs), is presented, with the focus on the tunable anisotropic optical properties. Using a self-assembling bottom-up deposition method, the as-grown VANs present great promise in terms of structural flexibility and property tunability. Such broad tunability of functionalities is achieved through VAN designs, material selection, growth control, and strain coupling. The as-grown multi-phase VAN films also present enormous advantages, including wafer scale integration, epitaxial quality, sharp atomic interface, as well as designable materials and geometries. This review also covers the research directions with practical device potentials, such as multiplex sensing, high-temperature plasmonics, magneto-optical switching, as well as photonic circuits.
Optimization of Classification Prediction Performances of an Instrumental Odour Monitoring System by Using Temperature Correction Approach
Authors: Oliva Giuseppina , Zarra Tiziano , Massimo Raffaele , Senatore Vincenzo , Buonerba Antonio , Belgiorno Vincenzo , Naddeo Vincenzo
Published: June 2021

Odour emissions generated by industrial and environmental protection plants are often a cause of nuisances and consequent conflicts in exposed populations. Their control is a key action to avoid complaints. Among the odour measurement techniques, the sensory-instrumental method with the application of Instrumental Odour Monitoring Systems (IOMSs) currently represents an effective solution to allow a continuous classification and quantification of odours in real time, combining the advantages of conventional analytical and sensorial techniques. However, some aspects still need to be improved. The study presents and discusses the investigation and optimization of the operational phases of an advanced IOMS, applied for monitoring of environmental odours, with the aim of increasing their performances and reliability of the measures. Accuracy rates of over 98% were reached in terms of classification performances. The implementation of automatic correction systems for the resistance values of the measurement sensors, by considering the influence of the temperature, has been proven to be a solution to further improve the reliability of IOMS. The proposed approach was based on the application of corrective coefficients experimentally determined by analyzing the correlation between resistance values and operating conditions. The paper provides useful information for the implementation of real-time management activities by using a tailor-made software, able to increase and enlarge the IOMS fields of application.
Measuring Household Resilience to Cyclone Disasters in Coastal Bangladesh
Authors: Al-Maruf Abdullah , Jenkins J. Craig , Bernzen Amelie , Braun Boris
Published: June 2021

The main objective of this paper is to measure the level of household resilience to cyclone and storm surges in the coastal area of Bangladesh. We draw on four general disaster frameworks in terms of addressing household-level resilience to cyclones and storm surges. We use a composite indicator approach organized around four components: (1) household infrastructure (HI); (2) household economic capacity (HEC); (3) household self-organization and learning (HSoL), and; (4) social safety nets (SSN). Drawing on a household survey (N = 1188) in nine coastal union parishads in coastal Bangladesh purposively selected as among the most vulnerable places in the world, we use principal components analysis applied to a standardized form of the survey data that identifies key household resilience features. These household index scores can be used for the assessment and monitoring of household capacities, training, and other efforts to improve household cyclone resilience. Our innovative methodological approach allows us to (a) identify patterns and reveal the underlying factors that accurately describe the variation in the data; (b) reduce a large number of variables to a much smaller number of core dimensions of household resilience, and (c) to detect spatial variations in resilience among communities. Aggregated to the community level, our new index reveals significant differences in community cyclone resilience in different areas of the coastal region. In this way, we can show that shoreline and island communities, in particular, have significant deficits in terms of household resilience, which seem to be mutually reinforcing one another and making for lower resilience.
Development of an Optimized NDT Methodology for the Investigation of Ancient Greek Copper-Based Artifacts
Authors: Saint Amani-Christiana , Dritsa Vasiliki , Koui Maria
Published: June 2021

A multi-analytical non-destructive testing (NDT) methodology was applied to copper-based artifacts originated from various archaeological sites of Greece. X-ray fluorescence (XRF), fiber optics diffuse reflectance spectroscopy (FORS) and scanning electron microscopy coupled with an energy dispersive X-ray detector (ESEM-EDX) were used for the characterization of the alloys and the corrosion products. The key elements of the artifacts belonging to the Early Bronze Age (2700–2300 BC) were copper and arsenic, while tin bronze was used for the fabrication of the Late Bronze Age (1600–1100 BC) artifacts. The effectiveness of XRF for the determination of the bulk composition was confirmed by comparative study with the previously applied atomic absorption spectroscopy (AAS) and inductively coupled plasma–atomic emission spectrometry (ICP-AES) destructive techniques. Significant differences between the artifacts were revealed through the spectral measurement of their surface corrosion products color by FORS. ESEM-EDX provided information on the microstructure, the elemental composition of the corrosion layers and bulk, as well as the distribution of the corrosion products on the surface. Conclusively, the combined NDT methodology could be regarded as a valuable and appropriate tool for the elemental composition of the bulk alloy, thus leading to the classification of their historical period and the corrosion products, contributing significantly to their conservation–restoration.
Difference in Procedure-Related Risk of Miscarriage between Early and Mid-Trimester Amniocentesis: A Retrospective Cohort Study
Authors: Steinfort Kelly , Van Houtven Ellen , Jacquemyn Yves , Blaumeiser Bettina , Loquet Philip
Published: June 2021

Early amniocentesis (EA)—before 15 gestational weeks—is not recommended because of a high rate of miscarriages. Most studies performed amniocentesis at very early stages of pregnancy (11–13 weeks of gestational age). However, amniocentesis performed at 14 gestational weeks could be an important alternative to mid-trimester amniocentesis (MA) because it shortens the time period between the screening (non-invasive prenatal test (NIPT)) and the diagnostic test (amniocentesis). This study aimed to compare the procedure-related risk of miscarriage between MA (15 + 0 to 17 + 6 weeks of gestational age) and EA (14 + 0–6 weeks of gestational age). This is a multicentric, retrospective cohort study from 1 January 2007 to 21 November 2018, comparing the MA to the EA cohort. Procedure-related fetal loss is defined as spontaneous abortion occurring within 4 weeks of the procedure. Multiple gestations, amniocenteses performed after 17 or before 14 weeks, indications other than prenatal genetic diagnoses and procedures performed by less experienced gynaecologists were excluded. Complete outcome data were available for 1107 out of 1515 women (73.1%): 809 (69.9%) in the MA and 298 (83.2%) in the EA cohort. No significant difference was found (EA 0.82% vs. MA 0.36%; p = 0.646). The difference was 0.46% (odds ratio = 0.673; 95% confidence interval = 0.123–3.699). This study found no significant difference in the procedure-related risk of miscarriage when EA was compared to MA. EA might be considered a safe alternative, though further research is necessary.
Cutting Staff Radiation Exposure and Improving Freedom of Motion during CT Interventions: Comparison of a Novel Workflow Utilizing a Radiation Protection Cabin versus Two Conventional Workflows
Authors: Dankerl Peter , May Matthias Stefan , Canstein Christian , Uder Michael , Saake Marc
Published: June 2021

This study aimed to evaluate the radiation exposure to the radiologist and the procedure time of prospectively matched CT interventions implementing three different workflows—the radiologist—(I) leaving the CT room during scanning; (II) wearing a lead apron and staying in the CT room; (III) staying in the CT room in a prototype radiation protection cabin without lead apron while utilizing a wireless remote control and a tablet. We prospectively evaluated the radiologist’s radiation exposure utilizing an electronic personal dosimeter, the intervention time, and success in CT interventions matched to the three different workflows. We compared the interventional success, the patient’s dose of the interventional scans in each workflow (total mAs and total DLP), the radiologist’s personal dose (in µSV), and interventional time. To perform workflow III, a prototype of a radiation protection cabin, with 3 mm lead equivalent walls and a foot switch to operate the doors, was built in the CT examination room. Radiation exposure during the maximum tube output at 120 kV was measured by the local admission officials inside the cabin at the same level as in the technician’s control room (below 0.5 μSv/h and 1 mSv/y). Further, to utilize the full potential of this novel workflow, a sterile packed remote control (to move the CT table and to trigger the radiation) and a sterile packed tablet anchored on the CT table (to plan and navigate during the CT intervention) were operated by the radiologist. There were 18 interventions performed in workflow I, 16 in workflow II, and 27 in workflow III. There were no significant differences in the intervention time (workflow I: 23 min ± 12, workflow II: 20 min ± 8, and workflow III: 21 min ± 10, p = 0.71) and the patient’s dose (total DLP, p = 0.14). However, the personal dosimeter registered 0.17 ± 0.22 µSv for workflow II, while I and III both documented 0 µSv, displaying significant difference (p < 0.001). All workflows were performed completely and successfully in all cases. The new workflow has the potential to reduce interventional CT radiologists’ radiation dose to zero while relieving them from working in a lead apron all day.
The Dynamic Spillover Effects of Macroeconomic and Financial Uncertainty on Commodity Markets Uncertainties
Authors: Ben Haddad Hedi , Mezghani Imed , Gouider Abdessalem
Published: June 2021

The present paper has two main objectives: first, to accurately estimate commodity price uncertainty; and second to analyze the uncertainty connectedness among commodity markets and the macroeconomic uncertainty, using the time-varying vector-autoregressive (TVP-VAR) model. We use eight main commodity markets, namely energy, fats and oils, beverages, grains, other foods, raw materials, industrial meals, and precious metals. The sample covers the period from January 1960 to June 2020. The estimated commodity price uncertainties are proven to be leading indicators of uncertainty rather than volatility in commodity markets. In addition, the time-varying connectedness analysis indicates that the macroeconomic uncertainty has persistent spillover effects on the commodity uncertainty, especially during the recent COVID-19 pandemic period. It has also found that the energy uncertainty shocks are the main drivers of connectedness among commodity markets, and that fats and oils uncertainty is the influence driver of uncertainty spillovers among agriculture commodities. The achieved results are of important significance to policymakers, firms, and investors to build accurate forecasts of commodity price uncertainties.
DDR4 BER Degradation Due to Crack in FBGA Package Solder Ball
Authors: Waqar Muhammad , Bak Geunyong , Kwon Junhyeong , Baeg Sanghyeon
Published: June 2021

This paper measures bit error rate degradation in DDR4 due to crack in fine pitch ball grid array (FBGA) package solder ball. Thermal coefficient mismatch between the package and printed circuit board material causes cracks to occur in solder balls. These cracks change the electrical model of the solder ball and introduce parallel capacitance in the electrical model. The capacitance causes higher frequency attenuation and closes the data eye. As the data rate of the DDR4 increases there are more data eye closures. The data eye closure causes bit error rate (BER) degradation as the timing margin and voltage margin decreases. This degradation reduces the reliability of the system and causes more intermittent errors. DDR4 data line is loaded with a parallel capacitive element to mimic a crack in solder ball. The measured data eye shows a decrease in eye width. Bathtub plots are created for comparison of cracked solder ball and intact solder ball. The bathtub plots show the BER degradation due to crack in solder ball.
Dietary Fiber Modulates the Fermentation Patterns of Cyanidin-3-O-Glucoside in a Fiber-Type Dependent Manner
Authors: Yang Zixin , Huang Ting , Li Ping , Ai Jian , Liu Jiaxin , Bai Weibin , Tian Lingmin
Published: June 2021

The interactions between cell-wall polysaccharides and polyphenols in the gastrointestinal tract have attracted extensive attention. We hypothesized that dietary fiber modulates the fermentation patterns of cyanidin-3-O-glucoside (C3G) in a fiber-type-dependent manner. In the present study, the effects of four dietary fibers (fructose-oligosaccharides, pectin, β-glucan and arabinoxylan) on the modulation of C3G fermentation patterns were investigated through in vitro fermentation inoculated with human feces. The changes in gas volume, pH, total carbohydrate content, metabolites of C3G, antioxidant activity, and microbial community distribution during in vitro fermentation were analyzed. After 24 h of fermentation, the gas volume and total carbohydrate contents of the four dietary-fiber-supplemented groups respectively increased and decreased to varying degrees. The results showed that the C3G metabolites after in vitro fermentation mainly included cyanidin, protocatechuic acid, 2,4,6-trihydroxybenzoic acid, and 2,4,6-trihydroxybenzaldehyde. Supplementation of dietary fibers changed the proportions of C3G metabolites depending on the structures. Dietary fibers increased the production of short-chain fatty acids and the relative abundance of gut microbiota Bifidobacterium and Lactobacillus, thus potentially maintaining colonic health to a certain extent. In conclusion, the used dietary fibers modulate the fermentation patterns of C3G in a fiber-type-dependent manner.
Barley Protein Properties, Extraction and Applications, with a Focus on Brewers’ Spent Grain Protein
Authors: Jaeger Alice , Zannini Emanuele , Sahin Aylin W. , Arendt Elke K.
Published: June 2021

Barley is the most commonly used grain in the brewing industry for the production of beer-type beverages. This review will explore the extraction and application of proteins from barley, particularly those from brewers’ spent grain, as well as describing the variety of proteins present. As brewers’ spent grain is the most voluminous by-product of the brewing industry, the valorisation and utilisation of spent grain protein is of great interest in terms of sustainability, although at present, BSG is mainly sold cheaply for use in animal feed formulations. There is an ongoing global effort to minimise processing waste and increase up-cycling of processing side-streams. However, sustainability in the brewing industry is complex, with an innate need for a large volume of resources such as water and energy. In addition to this, large volumes of a by-product are produced at nearly every step of the process. The extraction and characterisation of proteins from BSG is of great interest due to the high protein quality and the potential for a wide variety of applications, including foods for human consumption such as bread, biscuits and snack-type products.
The Role of Neural Network for the Detection of Parkinson’s Disease: A Scoping Review
Authors: Alzubaidi Mahmood Saleh , Shah Uzair , Dhia Zubaydi Haider , Dolaat Khalid , Abd-Alrazaq Alaa A. , Ahmed Arfan , Househ Mowafa
Published: June 2021

Background: Parkinson’s Disease (PD) is a chronic neurodegenerative disorder that has been ranked second after Alzheimer’s disease worldwide. Early diagnosis of PD is crucial to combat against PD to allow patients to deal with it properly. However, there is no medical test(s) available to diagnose PD conclusively. Therefore, computer-aided diagnosis (CAD) systems offered a better solution to make the necessary data-driven decisions and assist the physician. Numerous studies were conducted to propose CAD to diagnose PD in the early stages. No comprehensive reviews have been conducted to summarize the role of AI tools to combat PD. Objective: The study aimed to explore and summarize the applications of neural networks to diagnose PD. Methods: PRISMA Extension for Scoping Reviews (PRISMA-ScR) was followed to conduct this scoping review. To identify the relevant studies, both medical databases (e.g., PubMed) and technical databases (IEEE) were searched. Three reviewers carried out the study selection and extracted the data from the included studies independently. Then, the narrative approach was adopted to synthesis the extracted data. Results: Out of 1061 studies, 91 studies satisfied the eligibility criteria in this review. About half of the included studies have implemented artificial neural networks to diagnose PD. Numerous studies included focused on the freezing of gait (FoG). Biomedical voice and signal datasets were the most commonly used data types to develop and validate these models. However, MRI- and CT-scan images were also utilized in the included studies. Conclusion: Neural networks play an integral and substantial role in combating PD. Many possible applications of neural networks were identified in this review, however, most of them are limited up to research purposes.
MMP-2 and MMP-9 Polymorphisms and Preeclampsia Risk in Tunisian Arabs: A Case-Control Study
Authors: Gannoun Marwa Ben Ali , Raguema Nozha , Zitouni Hedia , Mehdi Meriem , Seda Ondrej , Mahjoub Touhami , Lavoie Julie L.
Published: June 2021

The abnormal production of matrix metalloproteinases (MMPs), especially MMP-9 and MMP-2, plays a pivotal role in hypertensive disorders of pregnancy, and as such, can influence the development of preeclampsia. These alterations may result from functional genetic polymorphisms in the promoter region of MMP-9 and MMP-2 genes, which modify MMP-9 and MMP-2 expression. We investigated the association of MMP-9 polymorphism rs3918242 (-1562 C>T) and MMP-2 polymorphism rs2285053 (-735 C>T) with the risk of preeclampsia. This case–control study was conducted on 345 women with preeclampsia and 281 age-matched women with normal pregnancies from Tunisian hospitals. Genomic DNA was extracted from whole blood collected at delivery. Genotypes for -1562 C>T and -735 C>T polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). An increased frequency of heterozygous MMP-9 -1562 C/T genotype carriers was observed in women with preeclampsia compared to healthy controls (p = 0.03). In contrast, the MMP-2 -735 C>T polymorphism was not significantly different regarding frequency distribution of the allele and genotype between healthy pregnant women and women with preeclampsia. Our study suggests that the MMP-9 -1562 C/T variant, associated with high MMP-9 production, could be a genetic risk factor for preeclampsia in Tunisian women.
Ablation Modalities for Therapeutic Intervention in Arrhythmia-Related Cardiovascular Disease: Focus on Electroporation
Authors: McBride Shauna , Avazzadeh Sahar , Wheatley Antony M. , O’Brien Barry , Coffey Ken , Elahi Adnan , O’Halloran Martin , Quinlan Leo R.
Published: June 2021

Targeted cellular ablation is being increasingly used in the treatment of arrhythmias and structural heart disease. Catheter-based ablation for atrial fibrillation (AF) is considered a safe and effective approach for patients who are medication refractory. Electroporation (EPo) employs electrical energy to disrupt cell membranes which has a minimally thermal effect. The nanopores that arise from EPo can be temporary or permanent. Reversible electroporation is transitory in nature and cell viability is maintained, whereas irreversible electroporation causes permanent pore formation, leading to loss of cellular homeostasis and cell death. Several studies report that EPo displays a degree of specificity in terms of the lethal threshold required to induce cell death in different tissues. However, significantly more research is required to scope the profile of EPo thresholds for specific cell types within complex tissues. Irreversible electroporation (IRE) as an ablative approach appears to overcome the significant negative effects associated with thermal based techniques, particularly collateral damage to surrounding structures. With further fine-tuning of parameters and longer and larger clinical trials, EPo may lead the way of adapting a safer and efficient ablation modality for the treatment of persistent AF.
The Change of Fingolimod Patient Profiles over Time: A Descriptive Analysis of Two Non-Interventional Studies PANGAEA and PANGAEA 2.0
Authors: Ziemssen Tjalf , Schulze-Topphoff Ulf
Published: June 2021

(1) Background: Fingolimod (Gilenya®) was the first oral treatment for patients with relapsing-remitting multiple sclerosis (RRMS). Since its approval, the treatment landscape has changed enormously. (2) Methods: Data of PANGAEA and PANGAEA 2.0, two German real-world studies, were descriptively analysed for possible evolution of patient profiles and treatment behavior. Both are prospective, multi-center, non-interventional, long-term studies on fingolimod use in RRMS in real life. Data of 4229 PANGAEA patients (recruited 2011–2013) and 2441 PANGAEA 2.0 patients (recruited 2015–2018) were available. Baseline data included demographics, RRMS characteristics and disease severity. (3) Results: The mean age of PANGAEA and PANGAEA 2.0 patients was similar (38.8 vs. 39.2 years). Patients in PANGAEA 2.0 had shorter disease duration (7.1 vs. 8.2 years) and fewer relapses in the year before baseline (1.2 vs. 1.6). Disease severity at baseline estimated by EDSS and SDMT was lower in PANGAEA 2.0 patients compared to PANGAEA (EDSS difference 1.0 points; SDMT difference 3.3 points). (4) Conclusions: The results hint at an influence of changes in the treatment guidelines and the label on fingolimod patients profiles over time. Patients tended to have lower disease activity at fingolimod initiation, suggesting an earlier intervention. This indicates increased experience in using fingolimod for sub-optimally treated RRMS patients and a change in mindset towards an early treatment optimization.
Statins in COVID-19 Therapy
Authors: Olszewska-Parasiewicz Justyna , Szarpak Łukasz , Rogula Sylwester , Gąsecka Aleksandra , Szymańska Urszula , Kwiatkowska Maria , Jaguszewski Milosz J. , Sierpiński Radosław , Zaczyński Artur , Wierzba Waldemar , Kosior Dariusz A.
Published: June 2021

Inhibitors of 3-hydroxy-3methylgultaryl-coenzyme A reductase (statins) are one of the main groups of drugs used in preventing and treating cardiovascular diseases worldwide. They are widely available, cheap, and well-tolerated. Based on statins’ pleiotropic properties, including improvement of endothelial dysfunction, antioxidant properties, atherosclerotic plaque stabilization, and inhibition of inflammatory responses, it can be hypothesized that the use of statins, at least as an adjuvant in antiviral therapy, may be justified. All these effects might be especially beneficial in patients with COVID-19, suffering from endothelial dysfunction, microvascular and macrovascular thrombosis, and cytokine storm. Here, we review the recent data regarding the pathophysiology of SARS-CoV-2 activity in host cells, proposed COVID-19 therapy, the pleiotropic activity of statins, and statins in clinical trials in respiratory infections. According to the guidelines of the European and American Cardiac Societies, in patients with cardiovascular disease or high cardiovascular risk with concomitant COVID-19 it is recommended to continue statin treatment. However, the initiation of statin therapy de novo in COVID-19 treatment should only be done as part of a clinical trial.
Influence of Austempering Temperatures on the Microstructure and Mechanical Properties of Austempered Ductile Cast Iron
Authors: Bendikiene Regita , Ciuplys Antanas , Cesnavicius Ramunas , Jutas Audrius , Bahdanovich Aliaksandr , Marmysh Dzianis , Nasan Aleh , Shemet Liudmila , Sherbakov Sergei
Published: June 2021

The influence of the austempering temperatures on the microstructure and mechanical properties of austempered ductile cast iron (ADI) was investigated. ADI is nodular graphite cast iron, which owing to higher strength and elongation, exceeds mechanical properties of conventional spheroidal graphite cast iron. Such a combination of properties is achieved by the heat treatment through austenitization, followed by austempering at different temperatures. The austenitization conditions were the same for all the samples: temperature 890 °C, duration 30 min, and quenching in a salt bath. The main focus of this research was on the influence of the austempering temperatures (270 °C, 300 °C, and 330 °C) on the microstructure evolution, elongation, toughness, and fatigue resistance of ADI modified by certain amounts of Ni, Cu, and Mo. The Vickers and Rockwell hardness decreased from 535.7 to 405.3 HV/1 (55.7 to 44.5 HRC) as the austempering temperature increased. Optical images showed the formation of graphite nodules and a matrix composed of ausferrite; the presence of these phases was confirmed by an XRD diffraction pattern. A fracture surface analysis revealed several types of the mechanisms: cleavage ductile, transgranular, and ductile dimple fracture. The stress-controlled mechanical fatigue experiments revealed that a 330 °C austempering temperature ensures the highest fatigue life of ADI.
High-Temperature Elastic Properties of Yttrium-Doped Barium Zirconate
Authors: Iguchi Fumitada , Hinata Keisuke
Published: June 2021

The elastic properties of 0, 10, 15, and 20 mol% yttrium-doped barium zirconate (BZY0, BZY10, BZY15, and BZY20) at the operating temperatures of protonic ceramic fuel cells were evaluated. The proposed measurement method for low sinterability materials could accurately determine the sonic velocities of small-pellet-type samples, and the elastic properties were determined based on these velocities. The Young’s modulus of BZY10, BZY15, and BZY20 was 224, 218, and 209 GPa at 20 °C, respectively, and the values decreased as the yttrium concentration increased. At high temperatures (>20 °C), as the temperature increased, the Young’s and shear moduli decreased, whereas the bulk modulus and Poisson’s ratio increased. The Young’s and shear moduli varied nonlinearly with the temperature: The values decreased rapidly from 100 to 300 °C and gradually at temperatures beyond 400 °C. The Young’s modulus of BZY10, BZY15, and BZY20 was 137, 159, and 122 GPa at 500 °C, respectively, 30–40% smaller than the values at 20 °C. The influence of the temperature was larger than that of the change in the yttrium concentration.
Hygienic Characteristics and Detection of Antibiotic Resistance Genes in Crickets (Acheta domesticus) Breed for Flour Production
Authors: Grispoldi Luca , Karama Musafiri , El-Ashram Saeed , Saraiva Cristina Maria , García-Díez Juan , Chalias Athanasios , Barbera Salvatore , Cenci-Goga Beniamino T.
Published: June 2021

During the last ten years, the worldwide interest in using insects as food and feed has surged. Edible insects fall within the category of novel foods, i.e., the category of food not consumed in significant amounts in the European Union before 15 May 1997 (the date of entry into force of Regulation (EC) No. 258/1997, later repealed by Regulation (EU) No. 2283/2015). One of the most promising insect species to be raised for food is the house cricket (Acheta domesticus). In this study, the rearing of a stock of house crickets was studied over a period of four months. The microbiological quality of the farm was studied using swabs on the surface of the rearing boxes to analyze the trend over time of different populations of microorganisms (total aerobic mesophilic microbiota, Lactobacillus spp., enterococci, Staphylococcus spp., Enterobacteriaceae, total coliforms, Pseudomonas spp. and molds). The presence of four antimicrobial resistance genes (aph, blaZ, sul1, and tetM) was investigated by polymerase chain reaction. A production scheme was also developed in order to obtain a cricket-based flour, which was analyzed for its microbiological and chemical-centesimal profile. The results obtained in this study demonstrate that the contamination increases with time and that a proper management of the farming system for insects is of the utmost importance, as it is for conventional farm animals such as ungulates, poultry, and rabbits. The old-fashioned adage “all full, all empty” for the farming system summarizes the need for proper cleaning and disinfection of the structures at the end of each production cycle.
Comprehensive Thermal Analysis of Diamond in a High-Power Raman Cavity Based on FVM-FEM Coupled Method
Authors: Bai Zhenxu , Zhang Zhanpeng , Wang Kun , Gao Jia , Zhang Zhendong , Yang Xuezong , Wang Yulei , Lu Zhiwei , Mildren Richard P.
Published: June 2021

Despite their extremely high thermal conductivity and low thermal expansion coefficients, thermal effects in diamond are still observed in high-power diamond Raman lasers, which proposes a challenge to their power scaling. Here, the dynamics of temperature gradient and stress distribution in the diamond are numerically simulated under different pump conditions. With a pump radius of 100 μm and an absorption power of up to 200 W (corresponding to the output power in kilowatt level), the establishment period of thermal steady-state in a millimeter diamond is only 50 μs, with the overall thermal-induced deformation of the diamond being less than 2.5 μm. The relationship between the deformation of diamond and the stability of the Raman cavity is also studied. These results provide a method to better optimize the diamond Raman laser performance at output powers up to kilowatt-level.
Bioherbicides: An Eco-Friendly Tool for Sustainable Weed Management
Authors: Hasan Mahmudul , Ahmad-Hamdani Muhammad Saiful , Rosli Adam Mustafa , Hamdan Hafizuddin
Published: June 2021

Weed management is an arduous undertaking in crop production. Integrated weed management, inclusive of the application of bioherbicides, is an emerging weed control strategy toward sustainable agriculture. In general, bioherbicides are derived either from plants containing phytotoxic allelochemicals or certain disease-carrying microbes that can suppress weed populations. While bioherbicides have exhibited great promise in deterring weed seed germination and growth, only a few in vitro studies have been conducted on the physiological responses they evoke in weeds. This review discusses bioherbicide products that are currently available on the market, bioherbicide impact on weed physiology, and potential factors influencing bioherbicide efficacy. A new promising bioherbicide product is introduced at the end of this paper. When absorbed, phytotoxic plant extracts or metabolites disrupt cell membrane integrity and important biochemical processes in weeds. The phytotoxic impact on weed growth is reflected in low levels of root cell division, nutrient absorption, and growth hormone and pigment synthesis, as well as in the development of reactive oxygen species (ROS), stress-related hormones, and abnormal antioxidant activity. The inconsistency of bioherbicide efficacy is a primary factor restricting their widespread use, which is influenced by factors such as bioactive compound content, weed control spectrum, formulation, and application method.
Estimation of Yield, Photosynthetic Rate, Biochemical, and Nutritional Content of Red Leaf Lettuce (Lactuca sativa L.) Grown in Organic Substrates
Authors: Sarkar Md. Dulal , Rahman Md. Jahedur , Uddain Jasim , Quamruzzaman Md. , Azad Md. Obyedul Kalam , Rahman Md. Hafizur , Islam Md. Jahirul , Rahman Mohammed Saifur , Choi Ki-Young , Naznin Most Tahera
Published: June 2021

This study aimed to evaluate the effect of organic substrates on the growth yield, photosynthetic response, and nutritional profile of red leaf lettuce grown in different compositions of cocopeat (CP), sawdust (SD), and rice husk (RH). The result showed that the properties of substrates were influenced variably by their mixing ratios. The highest water holding capacity and moisture content were found in CP, and it provided the preferable pH, electrical conductivity, bulk density, and air-filled porosity in association with other categories of the substrate. Cocopeat-based media provides ample microclimate conditions in the root region of plants and increased their height, number of leaves, and fresh biomass components. The utmost dry biomass of plant parts also remarkably increased in CP; L*, a*, and b* chromaticity of leaves remained unchanged. The maximum chlorophyll content was attained in CP substrate, except for chlorophyll a/b, which was higher in RH. The net photosynthetic rate (PN), transpiration rate (E), and nitrate in leaves were enhanced substantially in CP, while it was lower in SD. Biochemical compositions and nutrients in leaves were likewise stimulated under the culture of cocopeat-based media. Results indicate that cocopeat, sawdust, and rice husk are a possible substrates mixture in a volume ratio of 3:1:1, which would be a better choice in the cultivation of red leaf lettuce.
Experimental Verification of CFD Simulation When Evaluating the Operative Temperature and Mean Radiation Temperature for Radiator Heating and Floor Heating
Authors: Mičko Pavol , Kapjor Andrej , Holubčík Michal , Hečko Dávid
Published: June 2021

The assessment of heating systems is not only interested in the efficiency of the heating system itself, but also in the quality of the environment that the heating system creates. The quality of the environment and the heat-humidity microclimate is closely related to thermal comfort. A suitable environment has a positive effect, for example, on the efficiency of work at the workplace. The range of temperatures, humidity and operating temperatures in workplaces is often also legally prescribed in such a way that there is no thermal discomfort for users in the heated space. In terms of savings, it is therefore best to use heating systems that can create a comfortable environment with the lowest possible energy costs. During their development, variations are possible with temperature gradients, the size of the heat exchange area, or the ratio of the radiant and convective components of heat transfer. When developing such systems, it is appropriate to consider CFD simulations. The comparison of the results of CFD simulation and experimental measurement is also in the following article, where the comparison of the operating temperature and the mean radiation temperature of two different heating systems in the model office is monitored.
COVID-19 Vaccine Acceptance, Hesitancy, and Resistancy among University Students in France
Authors: Tavolacci Marie Pierre , Dechelotte Pierre , Ladner Joel
Published: June 2021

The objectives were to explore, among university students, the level of COVID-19 vaccine acceptance, hesitancy, and resistancy and to determine the motivations and barriers, and the reasons that may change student vaccination decision making. An online observational cross-sectional study was conducted among students of a French university in January 2021 with questions about the intention to be vaccinated against COVID-19, the motivations and the barriers. The convenience sample included 3089 students, with a mean of age of 20.3 (SD = 1.9). To the question on the intention to vaccinate against the COVID-19, 58.0% of students reported that they would choose to have a vaccination, 17.0% reported that they would not and 25.0% were not sure. The main motivations for vaccine acceptance were “I don′t want to transmit COVID-19 to others”, the main barriers for vaccine resistance or hesitancy were “I prefer to wait until I have more experience with these new vaccines”. Age, female gender, being in first three years of study, studied sciences courses and neither sciences nor healthcare courses of study were significantly associated with a higher risk of vaccine hesitancy or resistancy. Self-estimated knowledge of conventional vaccines and COVID-19 vaccines, and confidence in efficiency and safety of conventional vaccination were associated with a lower risk of vaccine hesitancy or resistancy. It is relevant to disseminate evidence-based interventions to promote COVID-19 vaccine acceptability for college students, especially for the students in neither sciences nor healthcare courses of study, as college students will soon be eligible to receive a COVID-19 vaccine.
Antivaccine Movement and COVID-19 Negationism: A Content Analysis of Spanish-Written Messages on Twitter
Authors: Herrera-Peco Ivan , Jiménez-Gómez Beatriz , Romero Magdalena Carlos Santiago , Deudero Juan José , García-Puente María , Benítez De Gracia Elvira , Ruiz Núñez Carlos
Published: June 2021

During the COVID-19 pandemic, different conspiracies have risen, with the most dangerous being those focusing on vaccines. Today, there exists a social media movement focused on destroying the credibility of vaccines and trying to convince people to ignore the advice of governments and health organizations on vaccination. Our aim was to analyze a COVID-19 antivaccination message campaign on Twitter that uses Spanish as the main language, to find the key elements in their communication strategy. Twitter data were retrieved from 14 to 28 December using NodeXL software. We analyzed tweets in Spanish, focusing on influential users, most influential tweets, and content analysis of tweets. The results revealed ordinary citizens who ‘offer the truth’ as the most important profile in this network. The content analysis showed antivaccine tweets (31.05%) as the most frequent. The analysis of anti-COVID19 tweets showed that attacks against vaccine safety were the most important (79.87%) but we detected a new kind of message presenting the vaccine as a means of manipulating the human genetic code (8.1%). We concluded that the antivaccine movement and its tenets have great influence in the COVID-19 negationist movement. We observed a new topic in COVID-19 vaccine hoaxes that must be considered in our fight against misinformation.
Vessel rigidity, methods of its determination and practical importance in the work of a paramedic
Authors: Skvortsov Vsevolod Vladimirovich , Gorbach Arina Nikolaevna , Shtonda Daniil Alekseevich
Published: June 2021

Important in the pathogenesis of many diseases of the cardiovascular system plays a decrease in elasticity and stiffening of the large arteries. Currently, one of the leading places is devoted to the study of the role of the vascular wall in the pathogenesis of hypertension. A number of studies indicate that an increase in the rigidity of the arterial bed is an independent factor of cardiovascular risk: at the same time, the risk of coronary artery disease, stroke, dissecting aortic aneurysms, and overall mortality from cardiovascular diseases is higher.