bluuebunny/crossref_metadata_2025 huggingface dataset:
EFFECTIVENESS OF CAMPUS TEACHING PROGRAM IMPLEMENTATION BATCH 3 AT PUBLIC ELEMENTARY SCHOOL DURING THE TRANSITIONAL PERIOD OF COVID-19 PANDEMIC
Authors: Ardiani Rizka , Wijayanto Hendra
Published: March 2023

The purpose of this study was to determine the effectiveness of the teaching campus education program during the transition period of the Covid-19 pandemic, namely from an online learning process to an offline learning process. The method used in this research is a qualitative method with a descriptive type. Grade 1-6 students from Public Elementary School 02 Cawet were used as test subjects. Data was collected through observation, interviews and documentation. The learning process that switches from online to offline learning requires preparation and adaptation by schools, teachers, parents and students. The results of the study show that the effectiveness of offline learning is quite good, but to reduce student interaction, learning is carried out according to health protocols. The process, implementation and effectiveness of online learning still need to be improved in order to achieve the educational goals of students effectively in terms of teaching, application of technology adaptation and school administration.
RISK MANAGEMENT LEVEL DETERMINANTS IN VISEGRAD COUNTRIES – SECTORAL ANALYSIS
Authors: Belas Jaroslav , Gavurova Beata , Kubak Matus , Novotna Andrea
Published: March 2023

Increasing competition has emphasized the need for creating and using more effective strategies at the level of SMEs to achieve competitive advantage. SMEs face higher risks in terms of the globalization due to the limited resources as well as size. The objective of the study was to examine the determinants of risk management with an emphasis on the sector specifics of SMEs in the V4 countries. This enables the quantification of sectoral differences in relation to the socioeconomic determinants. The research sample included 1585 SMEs from the V4 countries. The data obtained from SME managers and owners were processed using the descriptive statistics and logistic regression. The results of the analyses confirm the existence of differences across SMEs concerning the risk management, which are determined by the country in which a given company operates, industry, gender and age of its manager or the owner of the company. The results of the study provide a valuable platform for the development of systems to measure and assess business risks in companies and to set up training systems. It will also enhance the development of international and national benchmarking indicators and the creation of international databases for comparative analyses.
Biodiversity surveys of grassland and coastal habitats in 2021 as a documentation of pre-war status in southern Ukraine
Authors: Skobel Nadiia , Borovyk Dariia , Vynokurov Denys , Moysiyenko Ivan , Babytskiy Andriy , Bednarska Iryna , Bezsmertna Olesia , Chusova Olha , Dayneko Polina , Dengler Jürgen , Guarino Riccardo , Kalashnik Kateryna , Khodosovtsev Alexander , Kolomiychuk Vitalii , Kucher Oksana , Kuzemko Anna , Shapoval Viktor , Umanets Olha , Zagorodniuk Natalia , Zakharova Maryna , Dembicz Iwona
Published: March 2023

This paper presents two sampling-event datasets with occurrences of vascular plants, bryophytes and lichens collected in May-June 2021 in southern Ukraine. We aimed to collect high-quality biodiversity data in an understudied region and contribute it to international databases and networks. The study was carried out during the 15th Eurasian Dry Grassland Group (EDGG) Field Workshop in southern Ukraine and the Dark Diversity Network (DarkDivNet) sampling in the Kamianska Sich National Nature Park. By chance, these datasets were collected shortly before the major escalation of the Russian invasion in Ukraine. Surveyed areas in Kherson and Mykolaiv Regions, including established monitoring plots, were severely affected by military actions in 2022. Therefore, collected data are of significant value in the context of biodiversity documentation. The knowledge about the biodiversity of this area will help to assess the environmental impact of the war and plan restoration of the damaged or destroyed habitats. The first preliminary analysis of collected data demonstrates the biodiversity richness and conservation value of studied grassland habitats.

We provide sampling-event datasets with 7467 occurrences, which represent 708 taxa (vascular plants, bryophytes and lichens) collected in 275 vegetation relevés. Amongst them, vascular plants are represented by 6665 occurrences (610 taxa), lichens - 420 (46) and bryophytes - 381 (51). Several new species were reported for the first time at the national or regional level. In particular, one vascular plant species (Torilis pseudonodosa) and two lichen species (Cladonia conista, Endocarpon loscosii) were new to Ukraine. One vascular plant (Stipa tirsa), two species of bryophytes (Rhynchostegium megapolitanum, Ptychostomum torquescens) and three species of lichens (Cladonia cervicornis, C. symphycarpa, Involucropyrenium breussi) were recorded for the first time for the Kherson Region. Additionally, these datasets contain occurrences of taxa with narrow distribution, specialists of rare habitat types and, therefore, represented by a low number of occurrences in relevant biodiversity databases and particularly in GBIF. This publication highlights the diversity of natural vegetation and its flora in southern Ukraine and raises conservation concerns.
Potential risk resulting from the influence of static magnetic field upon living organisms. Numerically simulated effects of the static magnetic field upon fatty acids and their glycerides
Authors: Ciesielski Wojciech , Kołoczek Henryk , Oszczęda Zdzisław , Soroka Jacek A. , Tomasik Piotr
Published: March 2023

Background: We attempt to recognise the effects of static magnetic field (SMF) of varying flux density on flora and fauna. For this purpose, the influence of static magnetic field is studied for molecules of octadecanoic (stearic), cis-octadec-9-enoic (oleic), cis,cis-octadec-9,12-dienoic (linoleic), all cis-octadec-6,9.12-trienoic (linolenic), trans-octadec-9-enoic – (elaidic), cis-octadec-11-enoic (vaccenic) and all trans-octadec-6,9,12-trienoic (trans-linolenic) acids as well as 1- and 2-caproyl monoglycerides, 1,2- and 1,3-caproyl diglycerides and 1,2,3-caproyl triglyceride. In such a manner we attempt to develop an understanding of the interactions of living cells with SMF on a molecular level.

Methods: Computations of the effect of real SMF 0.0, 0.1, 1, 10 and 100 AMFU (Arbitrary Magnetic Field Unit; here 1AMFU > 1000 T) flux density were performed in silico (computer vacuum), involving advanced computational methods.

Results: SMF polarises molecules depending on applied flux density It neither ionises nor breaks valence bonds at 0.1 and 1 AMFU. In some molecules under consideration flux density of 10 and 100AMFU some C-H and C-C bonds were broken. Some irregularities were observed in the changes of positive and negative charge densities and bond lengths against increasing flux density. They provide evidence that molecules slightly change their initially fixed positions with respect to the force lines of the magnetic field. The length of some bonds and bond angles change with an increase in the applied flux density providing, in some cases, polar interactions between atoms through space.

Conclusions: SMF destabilizes lipid acids and caproyl glycerides irregularly against increasing flux density. That irregularity results from the ability of those molecules to twist out of the initially established SMF plain and squeeze molecules around some bonds. In some molecules SMF flux density of 10 AMFU and above breaks some valence bonds and only in case of elaidic acid the trans-cis conversion is observed. Depending on the structure and applied flux density SMF either stimulates or inhibits metabolic processes of the lipids under study.
Ibudilast ameliorates experimentally induced colitis in rats via down-regulation of proinflammatory cytokines and myeloperoxidase enzyme activity
Authors: Oubaid Enass Najem , Abu-Raghif Ahmed , Al-Sudani Israa Mahdi
Published: March 2023

Objectives: This study was carried out to explore the possible anti-inflammatory effect of ibudilast on acetic acid-induced colitis in rats.

Methods: Fifty adult Wistar rats were separated into 5 groups, including the control group, acetic acid group, acetic acid + vehicle, acetic acid + sulfasalazine (100 mg/kg/day)group, and acetic acid + ibudilast (30 mg/kg/day) group. Colitis was induced in rats by the inter-rectal installation of 2 ml of 4% (v/v) acetic acid. Sulfasalazine and ibudilast were administered orally for ten days after 2 hours of induction.

Results: The treatment with ibudilast significantly reduced disease activity index (DAI), macroscopic colonic scores (MAC), and histopathological changes induced by acetic acid. Also, ibudilast markedly decreased the expression of proinflammatory markers (TNF-α and IL-1β) in colonic tissue. Moreover, ibudilast inhibited myeloperoxidase (MPO) enzyme activity that was increased by acetic acid.

Conclusion: Therefore, ibudilast may have a therapeutic effect in the management of ulcerative colitis.
Comment l’acculturation alimentaire influence-t-elle le bien-être des expatriés professionnels ?
Authors: Hellal-Guendouzi Raficka , Dekhili Sihem
Published: March 2023

• Objectifs de recherche Cet article se propose d’étudier comment l’acculturation alimentaire contribue au bien-être des expatriés professionnels et de leurs familles installés dans un nouvel environnement culturel d’accueil. • Méthodologie La méthode des récits de vie a été mobilisée. Dans ce cadre, 25 entretiens narratifs ont été menés auprès de foyers d’expatriés américains, britanniques et allemands temporairement installés en France. • Résultats Le processus d’acculturation alimentaire des expatriés se déroule en cinq phases : une phase de « lune de miel », de crise, de résistance, d’adaptation progressive et d’adaptation à maturité. En fonction des trajectoires individuelles de consommation et du processus d’acculturation alimentaire, quatre profils d’expatriés ont été relevés : les traditionnels, les expatriés dans leur « bulle », les intégrés voyageurs et les intégrés nostalgiques. Par ailleurs, différents aspects de l’acculturation alimentaire relevant des comportements d’achat et de consommation affectent le bien-être des expatriés selon leurs profils. • Implications managériales/sociétales Différents leviers d’actions à destination des professionnels de la distribution et des entreprises qui emploient des expatriés sont identifiés afin de minimiser la phase de crise et faciliter la phase d’adaptation progressive. • Originalité Cette recherche montre comment le bien-être des expatriés évolue au cours du séjour d’expatriation sous le prisme du processus dynamique et des phases de l’acculturation. Elle offre un éclairage sur le processus d’ajustement des expatriés qui favorise la satisfaction individuelle et familiale des expatriés.
Le contenu de la parodie affecte-t-il l’attitude envers l’homme politique parodié ? Une analyse de médiation modérée
Authors: El Hana Nadr , Sabri Ouidade
Published: March 2023

• Objectif Les parodies politiques subversives suscitent le rire en vue de discréditer un politicien. Elles sont de plus en plus populaires sur les médias sociaux, menaçant ainsi l’évaluation du politicien parodié. Cependant, toutes les parodies politiques ne causent pas les mêmes préjudices. Dans ce contexte, notre recherche s’intéresse aux facteurs influençant l’évaluation de la parodie et les types de public qui pourraient y être réceptifs. • Méthodologie En nous appuyant sur deux expérimentations, nous analysons deux variables clés qui caractérisent les réactions aux parodies politiques et leur toxicité : le contenu de la parodie et la prédisposition envers l’homme politique parodié. • Résultats En s’inscrivant dans le champ de recherche qu’est le marketing politique, nous démontrons que, par rapport à une parodie politique reposant sur une attaque visant la personne, une attaque visant le programme, les actions ou les idées du politicien parodié endommagera davantage l’attitude à son égard via les rôles médiateurs de l’éthique perçue et de l’attitude envers la parodie. • Implications managériales/sociétales Nous identifions les parodies les plus dommageables du point de vue du politicien parodié et nous proposons des recommandations managériales tant pour les politiciens que pour les parodistes. • Originalité Nous montrons que les attaques à caractère personnel déclenchent des effets contre-productifs, entraînant une évaluation plus positive de l’homme politique parodié parmi ses détracteurs en raison du caractère non éthique d’une telle pratique.
J’ai reçu l’amour en héritage : transmission intergénérationnelle et conflits internes de valeurs dans le mouvement des coopératives d’habitants
Authors: Belaid Ons , Capelli Sonia , Sabadie William
Published: March 2023

• Objectif Les coopératives d’habitants sont un mouvement de consommateurs qui souhaitent changer le rapport à l’immobilier pour limiter les excès de ce marché. Sans renoncer à la propriété, ces coopératives hybrident deux systèmes de valeur (l’un basé sur la valeur économique, l’autre sur celle des liens sociaux) qui induisent des conflits internes de valeur pour les membres de ce mouvement. L’objectif de cet article est d’étudier les valeurs liées à la transmission dans ce mouvement de consommateurs et les éventuels conflits qui en découlent. • Méthodologie Une ethnographie est menée auprès de l’ensemble des membres d’une coopérative d’habitants pendant trois ans. Les données qualitatives sont collectées via une observation participante et non-participante, des entretiens semi-directifs et une étude documentaire. Elles sont traitées à l’aide d’une analyse thématique de contenu. • Résultats Les membres du mouvement renoncent globalement à transmettre une partie de leur capital économique à la génération familiale pour léguer un capital socio-culturel aux générations futures. Pourtant ils mettent en place trois stratégies pour résoudre les conflits qui émergent suite à ce choix. • Implications managériales Nos travaux permettent d’expliquer les freins au développement de l’innovation de l’habitat coopératif et de proposer des solutions pour soutenir ce modèle. • Originalité Alors que les mouvements de consommateurs ont été étudiés pour leur influence sur l’achat et l’usage des biens, notre recherche s’intéresse à leur transmission. Elle met en avant des conflits de valeur au sujet de la transmission des biens au sein d’un mouvement qui hybride deux régimes de valeur.
Les locutions auxiliaires modales à base nominale
Authors: Gosselin Laurent
Published: March 2023

L’article porte sur les semi-auxiliaires de modalité qui, au plan morphosyntaxique, se présentent sous la forme de locutions semi-figées construites autour d’un nom, et qui, du point de vue sémantique, expriment une valeur modale. Exemples : avoir la possibilité de , se trouver dans l’obligation de , se faire un devoir de , avoir l’honnêteté de , avoir le plaisir de… Après avoir précisé le statut et le rôle de ces locutions au sein des périphrases verbales, et rappelé les critères de classification des modalités, nous proposons un classement de ces locutions en fonction des modalités qu’elles expriment. Cela nous permet de mettre au jour les principes qui guident leur interprétation.
Exploring the Effectiveness of Telehealth Simulation in a Standardized Patient Scenario
Authors: Taylor Pamela H. , Coulter Katherine
Published: March 2023

Background:

This study evaluated transitioning face-to-face standardized patient (SP) competencies for nurse practitioner (NP) students to telehealth SP (TSP) competencies. Given the effects of coronavirus disease 2019 on clinical nursing education, faculty need evidence-based strategies that offer flexibility and high-quality learning experiences for students.

Method:

SP grade rubrics for NP students (
n
= 53) who completed either face-to-face or TSP examinations were compared to determine whether there were any differences in overall mean score, history taking, physical examination, final diagnosis, and documentation between the two groups.

Results:

A two-tailed independent sample t test examined whether the mean scores for the variables differed between face-to-face SP and TSP competencies.

Conclusion:

Overall results indicated the SP competencies were similar between the two groups. This confirms that both options of SP competencies for family NP students are acceptable.

[
J Nurs Educ
. 2023;62(3):162–166.]
Dynamic analysis of reaction-diffusion dual carbon model considering economic development in China
Authors: Hou Yanchuang , Wei Chunyue , Ding Yuting
Published: March 2023

In this paper, a reaction-diffusion dual carbon model associated with Dirichlet boundary condition is proposed under the influence of economic development in China. First, we enumerate and analyse some influencing factors of carbon emission and carbon absorption, and select economic development as the influence factor of carbon emission. Second, we establish a model associated with dual carbon and analyse the existence and stability of equilibrium and the existence of bifurcations. Finally, we analyse and predict for the value of parameters. Numerical simulations are presented to support our theory results. Combined with theoretical analysis and numerical simulations, we obtain that China can achieve carbon peak before 2030. If we want to achieve carbon neutral before 2060, it requires efforts from all of parts of society. Therefore, we put forward some practical suggestions to achieve carbon neutrality and carbon peak on schedule in China for the next few decades.
An efficient, lightweight MobileNetV2-based fine-tuned model for COVID-19 detection using chest X-ray images
Authors: Velu Shubashini
Published: March 2023

In recent years, deep learning's identification of cancer, lung disease and heart disease, among others, has contributed to its rising popularity. Deep learning has also contributed to the examination of COVID-19, which is a subject that is currently the focus of considerable scientific debate. COVID-19 detection based on chest X-ray (CXR) images primarily depends on convolutional neural network transfer learning techniques. Moreover, the majority of these methods are evaluated by using CXR data from a single source, which makes them prohibitively expensive. On a variety of datasets, current methods for COVID-19 detection may not perform as well. Moreover, most current approaches focus on COVID-19 detection. This study introduces a rapid and lightweight MobileNetV2-based model for accurate recognition of COVID-19 based on CXR images; this is done by using machine vision algorithms that focused largely on robust and potent feature-learning capabilities. The proposed model is assessed by using a dataset obtained from various sources. In addition to COVID-19, the dataset includes bacterial and viral pneumonia. This model is capable of identifying COVID-19, as well as other lung disorders, including bacterial and viral pneumonia, among others. Experiments with each model were thoroughly analyzed. According to the findings of this investigation, MobileNetv2, with its 92% and 93% training validity and 88% precision, was the most applicable and reliable model for this diagnosis. As a result, one may infer that this study has practical value in terms of giving a reliable reference to the radiologist and theoretical significance in terms of establishing strategies for developing robust features with great presentation ability.
Demanda Gold sandstone (Burgos): effect of consolidation and ageing tests on its petrographic and petrophysical properties
Authors: Gómez-Marfil A. , Varas-Muriel M.J.
Published: March 2023

Sandstones have been widely used in construction for their abundance, aesthetics, and ease of extraction. To determine sandstones’ quality, it is essential to analyse their petrographic and petrophysical properties and sensitivity (durability and conservation) to environmental agents. This paper evaluates the physical-mechanical changes undergone by Sierra de la Demanda (Burgos, Spain) sandstone under combined and induced water and salt aggression and assesses ESTEL 1100’s effectiveness and suitability as a treatment. This sandstone is porous, permeable, dense and quartz-rich with high hardness and strength. The treatment improved its petrophysical properties by modifying its pore geometry and connectivity, reducing absorbency, permeability and anisotropy, and further increasing its hardness and resistance. Salts did not substantially modify its properties as its porosity type absorbed the crystallisation pressure. Ultimately, its pore system and predominantly quartz composition make it a high-quality, weather-resistant material compatible with the treatment applied.
Economic Evaluation Rationalities: How Life Trajectories Shape Beach Commerce as Seen Through the Bourdieusian Framework
Authors: Sartore Marina de Souza , Leite Elaine da Silveira , Rodrigues Cae
Published: March 2023

Economic Evaluation Rationalities (EERs) of beach bar owners are central to the design of different patterns of commercial beaches. We ran multiple correspondence analysis based on data from 16 interviews with beach bar owners in Aracaju (Brazil) regarding their age, gender, educational status, origin, business longevity, use of social media, use of TripAdvisor and the way they measure customer’s satisfaction. The results showed three types of rationalities: word-of-mouth, expert recommendation and independent online evaluation. We also found that social, technological, generational and cultural capitals are active for the distribution of these three types of rationalities in the beach bar sub-field. The research framework can be replicated in different sets of commercial beaches, allowing for comparison, especially in terms of free and/or paid uses of the beach and the degree to which beach bar owners submit themselves to international trends in tourism management.
Cannabinoid Efficacy for Developmental Epileptic Encephalopathy (DEE) Intractable Seizure Control
Authors: Acker Courtney R. , Zeine Rana R.
Published: March 2023

Seizures in 20-30% of epileptics are resistant to treatment with anti-epileptic drugs (AED). This chapter evaluates studies on the efficacy of cannabinoids as adjunctive therapy for control of intractable seizures, with a focus on developmental epileptic encephalopathies (DEE). A systematic review conducted by literature search through PubMed, EBSCO, and ProQuest electronic databases identified 16 studies in the last seven years. Moderate- to low-certainty of evidence supports the benefits of cannabinoids in reducing seizure frequency, intensity, duration, and epileptics' overall condition in many types of intractable seizures. Studies used either synthetic cannabidiol (CBD), highly purified plant-derived CBD (Epidiolex®), or less pure cannabis plant-based CBD oils, and extended over months to two years. Drug interactions of ingested CBD with concomitant valproate and clobazam could be avoided with sublingual CBD. Five percent of patients became seizure free. Future studies are warranted to optimize the anti-seizure effects of emerging cannabinoid treatment strategies.
Neurobiology of Cannabinoids and Medical Cannabis in Therapeutic Intervention for Multiple Sclerosis
Authors: Uzair Mohammad , Qaiser Hammad , Arshad Muhammad , Tahseen Saneela , Bashir Shahid
Published: March 2023

Cannabinoids, either phytocannabinoids or synthetic derivatives of medical cannabis, exhibit a variety of potential pharmacotherapeutic effects by acting on the endocannabinoid system, through interactions with the cannabinoid receptors (CB1, CB2, and other receptors). Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS) that causes spasticity, neuropathic pain, urinary bladder dysfunction, and sleep disturbance. Treatment with cannabinoids decreases the severity of disease in animal models of experimental autoimmune-Theiler's virus-induced-encephalomyelis or toxic demyelination. CBD anti-inflammatory effects decrease CNS inflammatory infiltrates, microglial activation, and cytokines IFN-γ, IL-Iβ, IL-17, or TNF-α, whereas Δ9-THC reduces spasticity. Add-on treatment with cannabinoids (Nabiximols, Sativex, equimolar concentration of THC:CBD) have demonstrated potential in alleviating the symptoms of patients with MS. This chapter reviews the evidence-based therapeutic effects of cannabinoids and their mechanisms of action relevant to MS.
History and Legislative Changes Governing Medical Cannabis in Indonesia
Authors: Komalasari Rita
Published: March 2023

This chapter presents the historical context of medical cannabis in Indonesia. First, the author sets out the history of legislative change—how cannabis law has changed over time and Indonesia's cannabis policy-making over the past 200 years. Cannabis that previously was not legal then was legal, and then not legal. Second, the author discusses recent studies regarding the benefits of natural and pharmaceutical-medical cannabis to patients with different conditions in Indonesia. These benefits include cannabis-based medicines for cancer pain, anxiety, and depression. This chapter illustrates the major changes that will be unfolding in countries worldwide regarding cannabis legislation and medical cannabis legalization in healthcare and medicine. This chapter gives an important perspective on the issues regarding the use of medical cannabis from a public health viewpoint. It gives a realistic discussion of the work that lies ahead for government and law enforcement agencies as cannabinoids become more widely accepted by the medical profession.
Creativity in the Gifted Classroom
Authors: Neal Tia M.
Published: March 2023

It is not enough for the creatively gifted children in the school systems to simply wonder about the world around them. It is the responsibility of educators to promote and encourage those wonderings so that gifted children can develop their creativity to their fullest potential. The objective of this chapter is to examine the various viewpoints of the nature of creativity. This chapter seeks to explore the following questions: (a) What is creativity? (b) Are academic giftedness and creative giftedness distinct concepts? (c) Can creativity be assessed? (d) What instructional strategies can be used within classrooms to enhance the creativity of gifted students? (e) How does one build a culture of creativity within the classroom?
Counseling Gifted Black Students
Authors: Owens Delila , Grey Lorise D.
Published: March 2023

Gifted students are often regarded as the privileged recipients of the best a school district has to offer. On the one hand, gifted students have access to the best teachers, the most rigorous curriculum, and the best postsecondary options. However, gifted Black students also face unique academic, social/emotional, and college/career challenges, and a lot of brilliant Black students are overlooked. A major thrust of this chapter is to identify some conditions or barriers that cause the underrepresentation of Black students in gifted education and offer recommendations to support student achievement.
Identification and Segmentation of Medical Images by Using Marker-Controlled Watershed Transformation Algorithm, XAI, and ML
Authors: Yesmin Tahamina , Acharjya Pinaki Pratim
Published: March 2023

To make human life easy and compact, XAI has developed a lot with more innovations and contributed its own share. To make a suitable treatment while diagnosed with brain tumour, one needs to classify the tumour and detect it in a proper way where the explained result is most important. With the help of different analysis processes where marker-based approaches can help in proper segmentation and noise reduction analysis, numerous imaging modalities exist for tumour detection that are utilized to identify tumours in the brain. One of the most important issues of XAI system is medical diagnosis through ML in medical image processing. In this chapter, the authors present a modified marker-controlled watershed transformation approach to detect brain tumour with XAI and machine learning approaches. They include CNN and data augmentation algorithms. Image pre-processing takes the main area to detect and diagnose disease and diagnose properly. The statistical measurements have been introduced to get the mathematical abstractions of different approaches for result analysis.
Identification of Vermamoeba vermiformis and Tetramitus sp. Isolated from the Gills of Oreochromis sp. (Red Hybrid Tilapia)
Authors: Wan Noor Azmi Wan NorNadhirah , Hashim Fatimah , Suhaili Zarizal , Misbah Suzana , Zakaria Nor Hafizah
Published: March 2023

This study is the first report of an infestation of amoeba in the gills of asymptomatic Oreochromis sp. from the Manir River, Terengganu, Malaysia. The results confirmed the presence of two species of amoeba, Vermamoeba vermiformis and Tetratmitus sp., based on the 18 ribosomal RNA gene sequences with 99% sequence similarity. Morphological observations using light and scanning electron microscopy supported the findings, demonstrating both amoeba species' specific features and locomotions. In the trophozoite stage, the mean size of V. vermiformis in the locomotive form was between 25.55 µm in length and 4.17 µm in width. The mean size of Tetramitus sp. was 20.96 µm in length and 8.66 µm in width. The diameter of the cyst for V. vermiformis was 4.67 µm and Tetramitus sp. was 3.37 µm. V. vermiformis was characterized by cylindrical trophozoites and single monopodial morphology. Tetramitus sp. showed a limax-type and various cell shapes. Infestation of the amoeba was also confirmed by histopathological observation in the lamella region with distinct amoeba characteristics compared to the lamellae's epithelial tissue. These findings revealed the presence of V. vermiformis and Tetramitus sp. infestation in the gills of Oreochromis sp. and its potential pathogenic activity, whilst the symbiosis interaction in Oreochromis sp. is still unidentified.
Expression of SARS-CoV-2 Nucleocapsid (N) Recombinant Protein Using Escherichia coli System
Authors: Ansari Rizki Aulia , Saepuloh Uus , Mariya Silmi , Yuliana  , Noviana Rachmitasari , Suparto Irma Herawati , Darusman Huda Shalahudin
Published: March 2023

One of the main antigen that can be used for serological testing is the nucleocapsid (N) which is the most abundant viral-derived protein in SARS-CoV-2 where this virus can cause COVID19 disease. The aim of this study was to develop the SARS-CoV-2 N recombinant protein using Escherichia coli expression system. A total of 1,089 nucleotides encoding 362 amino acids of SARS-CoV-2 N was cloned to pET-14b vector. The plasmid then expressed in E. coli BL21 (DE3) and induced with 1.0 mM IPTG (Isopropyl-β-d-1-thiogalactopyranoside). The cell was harvested using denaturation lysis buffer due to inclusion body formation of SARS-CoV-2 N protein. Dialysis processed and concentrated using PEG-6000 resulted 0.992 mg/ml protein yield. Analysis of SARS-CoV-2 N recombinant protein using SDS-PAGE technique showed approximately 37.0 kDa specific band target protein. Application of this SARS-CoV-2 N recombinant protein to vaccinated and non-vaccinated antibody serum samples using ELISA technique indicated the significant result of optical density mean at 0.603 and 0.135, respectively. This study revealed that the production of SARS-COV-2 N recombinant protein could be carried out in E. coli expression system under denatured conditions, therefore the methods are more effective in producing the protein as a basic material in immuno-diagnostic assay.
Phytoremediation of Acid Mine Drainage with Melaleuca cajuputi, Nauclea orientalis, and Vetiveria zizanioides in Floating Treatment Wetland
Authors: Sekarjannah Fitri Arum , Mansur Irdika , Abidin Zaenal , Fauzi Anas Miftah
Published: March 2023

The formation of acid mine drainage (AMD) is a common environmental problem in the mining industry. Its passive management through wetland construction has gained more consideration in recent years. However, the application in the field is constrained by the large area and relatively shallow depth. Indonesia has no passive technology to neutralize AMD in deep water. One solution is to apply a floating treatment wetland (FTW) system. Therefore, this study aimed to determine the ability of several hyperaccumulator plants, such as Melaleuca cajuputi, Nauclea orientalis, and Vetiveria zizanioides, to neutralize AMD using a floating system by conducting FTW trials. The method used was a Completely Randomized Design (CRD) with 3 treatments and control/without plants. Each treatment had 3 replications, resulting in 12 experimental units. The results showed that the FTW with or without plants could increase pH and decrease dissolved Mn by 75.31-90.74%. Heavy metals were chelated by organic matter, absorbed by plants, and deposited in the form of metal sulfides. The results also indicated that besides having a positive effect on pH and heavy metal reduction, the organic-based floating wetland increased biological oxygen demand (BOD) from 61.08-79.71%.
Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines
Authors: Hasbullah Omat Rachmat , Fiddiyanti Ilma , Handayani Dewi Ratih , Sutedja Endang , Ismono Darmadji , Hidajat Nucki Nursjamsi , Widowati Wahyu , Afifah Ervi , Kusuma Hanna Sari Widya , Rizal Rizal , Alamsyah Firman , Taruno Warsito P.
Published: March 2023

Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-contact electric field has anti-proliferative effect on breast cancer cells, in vitro and in vivo. In this study, we were interested studying alternating current electric field effects on osteosarcoma cells progression as well as its potential cytotoxic effects. MG-63 human osteosarcoma cells were cultured and treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, and caspase-9 were analyzed using real-time qPCR method. Apoptotic index was measured using flow-cytometry assay. Apoptosis was observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis through internal pathways were shown by the increase of caspase-3 (p = 0.015) and caspase-9 (p = 0.001) levels, but not caspase-8 (p = 0.080). This treatment has successfully reduced the number of living osteosarcoma cells by 14.7% (p = 0.000) and increased cell death up to 4.26% (p = 0.055). Apoptotic index was markedly increased to 16% (p = 0.001). 200 kHz non-contact electric field exposure can disrupt osteosarcoma progression through disruption of normal cell cycle via p53-independent p21 pathway and induction of apoptosis.
Growth Patterns and Mortality of Lobster Panulirus ornatus from the Catch of Bottom Gill Net Fishers in the Western Waters of Tarakan Island
Authors: Indarjo Agus , Salim Gazali , Maryanto Thonas Indra , Ngungut  , Linting Laurha Annisa , Firdaus Muhammad , Rozi  , Rukisah
Published: March 2023

Large-bodied Panulirus ornatus lobsters are economically important because they are an aquatic biota. It is one of the most important commodities in the city of Tarakan. The aim is to analyze the growth patterns and mortality of P. ornatus from the West Waters of Tarakan City. Method using a quantitative descriptive. The purposive sampling method was used by dragging the gill net fishing gear to the P. ornatus lobster ground area, and the collection was carried out 16 times in 7 months (December 2021-July 2022). The results of P. ornatus showed that the male sex ratio was higher than the female. The growth model was allometrically negative, and the criteria for the condition index were fat and thin. The growth pattern of Model Von Bertalanffy males is slower than females. However, the growth rate to achieve asymptotic growth for males is faster than for females. The total mortality, catch mortality, and exploitation rate of males was higher than females. However, the mortality of the female nature was higher than that of males. P. ornatus should be managed in the waters of West Tarakan using the domestication and restocking technique to increase sustainability.
Correlation of CDX2 Protein Expression with Clinicopathologic Features and Survival Rate in Iraqi Patients with Colorectal Cancer
Authors: Jahil Muntadher J. , Zayed Karrar S.
Published: March 2023

Predicting the prognosis of colorectal cancer (CRC) is challenging since these tumors exhibit a wide range of biological behaviors. It has been hypothesized that caudal-related homeobox gene 2 (CDX2), which is vital for intestinal growth and maintenance, has a tumor-suppressing effect and promising role in CRC prognosis but studies are still controversial. This study used the immunohistochemical (IHC) staining method to determine the expression of the CDX2 protein in mucinous and non-mucinous CRC adenocarcinoma, as well as in normal colorectal tissues as a control, and correlate this expression with clinicopathological features such as grade, tumor distant metastasis, tumor site, histological type, lymph node metastasis, tumor invasion, sex, age, and rate of 4 years Overall survival (OS) after diagnosis. A total of sixty three tissue samples were obtained from CRC patients (58.90±14.94) years and embedded in wax and thirty-seven normal non-tumoural colorectal tissue samples with (56.43±12.28) years as a control group. CDX2 protein expression decreased significantly (p<0.05) in CRC patients than control, advanced age, mucinous pattern of CRC, moderate and poorly differentiated grades, lymph node metastasis, advanced tumor invasion (T3, T4), and organs metastasis. Moreover, the (OS) for patients with low CDX2 expression was (17.943±1.7) months compared to (33.431±2.7) months for those with high CDX2 expression (p = 0.0001). This study concluded that protein expression of CDX2 is regarded as a prognostic and diagnostic marker for CRC patients.
Enhancing Solubility of Recombinant Plasmodium Lactate Dehydrogenase (pLDH) Using Combination of Cold-Inducible Expression System and Cold-Stirred Bioreactor
Authors: Alimuddin  , Ali Muhamad , Alim Sahrul , Amin Muhamad
Published: March 2023

A major drawback associated with an expression of a high-level Plasmodium Lactate Dehydrogenase (pLDH) using Escherichia coli is the low solubility due to the formation of an inclusion body (IB). This study aimed to develop a suitable protocol for enhancing the solubility of pLDH expressed in E. coli. Firstly, a pLDH-encoding gene was amplified from the blood sample of malaria-infected patients and ligated into pBlueScript II KS+ for sequencing. Afterward, the pLDH gene was digested and cloned into pColdTF for expression. The recombinant plasmid was transformed into the E. coli BL21 (DE3) RIPL Codon Plus Strain. Then, the bacterial host was initially cultured at 37°C until reaching optical density (OD) at 600 nm: 0.5. Thereafter, the growth temperature was lowered to 15°C, followed by the addition of 0.1 mM IPTG into the culture medium for inducing pLDH expression. Thereafter, the bacterial hosts were cultured in a cold-stirred bioreactor (15°C). The result showed that a combination of the low culture conditions (15°C) and a low amount of IPTG increased the solubility of pLDH. This result suggests that this protocol can be a convenient method for generating high-quality recombinant protein using the E. coli system.
Samomor in Cankarjev roman Martin Kačur
Authors: Zupan Sosič Alojzija
Published: March 2023

Samomor je v Sloveniji kljub visoki stopnji samomorilnosti še vedno tabu tema, zato sem se mu natančneje posvetila v pregledu filozofskih stališč in klasifikacij prostovoljne smrti. V interpretativno-analitični raziskavi sem Kačurjevo smrt v Cankarjevem romanu Martin Kačur utemeljila kot samomor, ki sem ga premislila z različnih perspektiv. Upoštevala sem Schopenhauerjevo filozofsko strpnost do samomora skozi etiko sočutja, Nietzschejevo upravičenje samomora kot izraza človekove svobode v svetu, Freudovo razlago povezave nagona smrti in življenja ter tri stopnje filozofskega prepričanja oziroma razpoloženja. To so idealizem, nihilizem in fatalizem, razporejeni v nenehno dialoško razmerje, prek katerih prestopamo z zgodbene na pripovedno raven. Pri upoštevanju realistične (postopen propad Kačurja) in simbolistične (slutenjska logika simbolov) poetike sem za dokaz samomora uporabila različne klasifikacije, po katerih je Kačurjev samomor subtilna povezava egoističnega ali sebičnega in logičnega ali nihilističnega ter krotkega in nezavednega samomora. Medtem ko povezava egoističnega in logičnega filozofsko utemelji Kačurjevo odločitev, vpliva preplet nehotenega in krotkega samomora na bralno empatijo. Z njeno pomočjo tragičnega propada altruističnega Kačurja, podobnega večini Cankarjevih glavnih likov, ne poskušamo razumeti samo skozi tematizacijo nesrečnega idealista, ampak tudi s posebno poetiko (avstrijske) moderne, ki je na začetku 20. stoletja smrti namenila posebno pozornost.
Museum as technology
Authors: Serhii Serhii
Published: March 2023

There are no complex studies in the field of cultural theory on the effectiveness of the museum. It is not clear what are the main versions of the sociocultural purpose of the museum. It has not been revealed which sociocultural applications of the museum are harmful to the institution and society as a whole, and which are not. The meta-problem of research is caused by the fact that the empirical question of choosing the best strategy for the functioning of the museum has not been brought to the theoretical level. There are only particular scientific discourses that have not passed the procedure of scientific criticism and logical-deductive testing. Thus, the scientific problem of the sociocultural purpose of the museum in view of its institutional specifics can be solved by formulating scientific theories that correspond to the particular directions of empirical use of the museum.
Generation Z perspectives on museum sustainability using Q methodology
Authors: Ásványi Katalin , Fehér Zsuzsanna
Published: March 2023

Museums and researchers require knowledge of how museums think about and practice sustainability to understand how sustainability considerations can further be incorporated and institutionalised into museum practice on four pillars: environmental, social, economic and cultural. A systematic literature review was carried out to explore the theoretical background of sustainable museums. This study, which used Q methodology, was designed to determine museum visitors’ preferences related to the most important sustainability elements of museums. The participants in the study were 24 museum visitors from Generation Z in Hungary. Data were collected through 37 Q statements. According to the findings of the research, young museum visitors can be distinguished into three groups (factors): Conscious, Experience-seeking, and Enthusiastic-to-learn visitors.
Performance Analysis of GFDM System in 5G Cellular Networks
Authors: Jeganlal R. , Jiji G. , Mariyal C. , Sehina H. , Raj T. Ajith Bosco
Published: March 2023

Orthogonal Frequency Division Multiplexing (OFDM) is employed in the current 4G cellular technologies. Because of its resistance to multipath propagation channels, OFDM is a popular multicarrier modulation technique. Although OFDM is one of the effective approaches for a 4G network, out-of-band radiation, inter-symbol interference (ISI), and inter-carrier interference make this method unsuitable for 5G. (ICI). A new approach or next-generation cellular network, i.e., very high data rate, low latency, greater throughput, more device connectivity, and improved device-to-device communication, should be used to deliver effective and dependable communication. The system introduces generalized frequency division multiplexing (GFDM). In GFDM, a single cyclic prefix is utilized throughout the frame and an adjustable pulse-shaping filter can be used to monitor the transmitted signal’s out-of-band emission. Using this ISI, the multipath channel can be controlled. In the proposed system, with large signal constellations, higher-order modulation schemes, and advanced receiver structure, a very high data rate can be achieved. By using various iterative receiver structures the performance of the GFDM system in 5G cellular networks can be analyzed.
Blockchain-Based Medical Cyber Physical Systems With Decentralized Threshold signature Scheme
Authors: Zhou Xianfei , Cheng Hongfang , Li Min , Chen Fulong
Published: March 2023

Medical cyber physical systems are information applications of medical industry.A lagrge amount of medical data is stored in MCPS,and there are many challenges in the secure store and data sharing.Using blockchain technology into medical Cyber Physical system has become popular.Blockchain has remarkable features such as tamper proof and privacy protection, and has the function of protecting data in the medical Cyber Physical system.In this paper,we propose a hybrid blockchain,which applied private blockchain and consortium blockchain, After the medical source data is hashed, a hash tree is generated and stored in the private chain of the hospital. The hospital server extracts information to build a new transaction on the consortium chain.the system ensure the secure storage and fast access of data.Still,a threshold signature system is proposed.Aiming at the situation that medical accidents are easy to occur in multidisciplinary joint consultation in the medical process, this paper proposes to use threshold signature for joint consultation.Using the security and tamper-proof of the threshold signature, when the consensus is reached,treatment can be carried out and the medical data is uploaded to the consortium blockchain. The security analysis and performance analysis show that the scheme has advantages in safety and performance and is suitable for the medical environment.
Secured Drone Communication Based on Esalsa20 Algorithm
Authors: Jomaa Ibtesam , Saleh Worud Mahdi , Ismail Rasha Rokan , Hussien Saja Huzber
Published: March 2023

The Unmanned Aerial Vehicle (UAV) (sometimes known as a ""drone"") is used in a variety of fields. Unfortunately, as they become more popular and in demand, they become more vulnerable to a variety of security threats. To combat such attacks and security threats, a proper design of a robust security and authentication system based on and stream cipher lightweight salsa20 algorithm with chaotic maps is required. By using a proposed key generation method which is based on a 1d Logistic chaotic map to produce a flight session key for a drone with a flight plan, and then records the flight session key and the drone’s flight plan in a central database that can be accessed. Finally, while the drone is flying, a GCS checks authentication of the current flight session based on the on flight session key and its flight plan as the message authentication code key to authenticate the drone by any flight session, and the drone after which uses salsa20 lightweight to cipher payload data to improve security Network Transfer of RTCM Messages over Internet Protocol Protocol (NTRIP) communication protocol and send it to GCS, and at last, a GCS verifies authentication of the current flight session based on the on flight session key and its flight plan as the message authentication code key to authenticate the drone. The proposed system is superior to other similar systems in terms of security and performance, according to the review.