bluuebunny/crossref_metadata_2025 huggingface dataset:
Robot-assisted end-effector-based gait training in chronic stroke patients: A multicentric uncontrolled observational retrospective clinical study
Authors: Mazzoleni Stefano , Focacci Antonella , Franceschini Marco , Waldner Andreas , Spagnuolo Chiara , Battini Elena , Bonaiuti Donatella
Published: February 2017

BACKGROUND: Until now studies report inconclusive results as regards the effectiveness of exclusive use of robot-assisted training and clinical indications in stroke patients.

OBJECTIVE: To evaluate if the only robot-assisted end-effector-based gait training can be feasible in chronic stroke subjects in terms of gait recovery.

METHODS: Five rehabilitation centers participated and one hundred chronic post-stroke patients were recruited. Patients underwent a robot-assisted end-effector-based gait training as only rehabilitation treatment.

6 Minute Walk Test, 10 Meter Walk Test, Timed Up and Go test, Modified Ashworth Scale, Motricity Index, Functional Ambulation Classification (FAC) and Walking Handicap Scale were used as outcome clinical measure. Patients were divided into two groups: those assessed as FAC <3 (Group 1) and as FAC ≥ 3 (Group 2).

RESULTS: Statistically significant changes were observed in each clinical outcome measure. Significant changes were observed in Group 1 and in Group 2. Significant percentages of patients achieved Minimally Clinically Important Difference in 6 Minute Walk Test in Group 2 and Timed Up and Go test in Group 1.

CONCLUSIONS: Chronic stroke patients exposed to only robot-assisted end-effector-based gait training showed significant improvements in global motor performances, gait endurance, balance and coordination, lower limbs strength and even spasticity.
Impact of the Brain Injury Family Intervention (BIFI) training on rehabilitation providers: A mixed methods study
Authors: Meixner Cara , O’Donoghue Cynthia R. , Hart Vesna
Published: February 2017

BACKGROUND: The psychological impact of TBI is vast, leading to adverse effects on survivors and their caregivers. Unhealthy family functioning may be mitigated by therapeutic strategies, particularly interdisciplinary family systems approaches like the well-documented Brain Injury Family Intervention (BIFI). Little is known about the experience of providers who offer such interventions.

OBJECTIVE: This mixed methods study aims to demonstrate that a structured three-day training on the BIFI protocol improves providers’ knowledge and confidence in working with survivors and families, and that this outcome is sustainable.

METHODS: Participants were 34 providers who participated in an intensive training and completed a web-based survey at four points of time. Quantitative data were analyzed via Wilcoxon signed-rank tests and binomial test of proportions. Qualitative data were analyzed according to rigorous coding procedures.

RESULTS: Providers’ knowledge of brain injury and their ability to conceptualize treatment models for survivors and their families increased significantly and mostly remain consistent over time. Qualitative data point to additional gains, such as understanding of family systems.

CONCLUSIONS: Past studies quantify the BIFI as an evidence-based intervention. This study supports the effectiveness of training and serves as first to demonstrate the benefit for providers short- and long-term.
Multiple mediation path model of pain’s cascading influence on physical disability in individuals with SCI from Colombia, South America
Authors: Perrin Paul B. , Paredes Alejandra Morlett , Olivera Silvia Leonor , Lozano Juan Esteban , Leal Wendy Tatiana , Ahmad Usman F. , Arango-Lasprilla Juan Carlos
Published: February 2017

BACKGROUND: Research has begun to document the bivariate connections between pain in individuals with spinal cord injury (SCI) and various aspects of health related quality of life (HRQOL), such as fatigue, social functioning, mental health, and physical functioning.

OBJECTIVE: The purpose of this study was to construct and test a theoretical path model illuminating the stage-wise and sequential (cascading) HRQOL pathways through which pain increases physical disability in individuals with SCI in a sample from Colombia, South America. It was hypothesized that increased pain would lead to decreased energy, which would lead to decreased mental health and social functioning, which both would lead to emotional role limitations, which finally would lead to physical role limitations.

METHODS: A cross-sectional study assessed individuals with SCI ( n = 40) in Neiva, Colombia. Participants completed a measure indexing various aspects of HRQOL.

RESULTS: The path model overall showed excellent fit indices, and each individual path within the model was statistically significant. Pain exerted significant indirect effects through all possible mediators in the model, ultimately suggesting that energy, mental health, social functioning, and role limitations-emotional were likely pathways through which pain exerted its effects on physical disability in individuals with SCI.

CONCLUSIONS: These findings uncover several potential nodes for clinical intervention which if targeted in the context of rehabilitation or outpatient services, could result in salubrious direct and indirect effects reverberating down the theoretical causal chain and ultimately reducing physical disability in individuals with SCI.
Association between gait variability, falls and mobility in people with multiple sclerosis: A specific observation on the EDSS 4.0-4.5 level
Authors: Kalron Alon
Published: February 2017

BACKGROUND: We previously demonstrated that gait variability increases throughout the disease process in people with multiple sclerosis (PwMS). While gait variability tends to remain steady in the lower levels of disability, a significant increase occurs once patients reach the moderate neurological level.

OBJECTIVE: To examine the relationship between variability of major spatio-temporal parameters of gait and falls, in PwMS with an expanded disability status scale score of 4.0 and 4.5.

METHODS: The study included 91 PwMS, 50 women. Gait variability was studied using an electronic mat. Clinical gait measures included the Two-minute walk test, Timed Up and Go test and the Timed 25-foot walk.

RESULTS: Fifty patients were classified as fallers, 41, non-fallers. The MS fallers presented a higher variability score in the step length (37.3% increase) and single support (28.2% increase) compared to participants in the non-fallers. Additionally, gait variability scores were significantly correlated with clinical walking tests. The strongest correlation scores were for variability of the step length. Pearson’s rho scores for the Timed Up and Go test, 2-min walk and Timed 25-foot walk were 0.541, – 0.448 and 0.425, respectively.

CONCLUSIONS: This study confirms that gait variability is a potential treatment target for PwMS moderately disabled, in order to decrease risk of falls.
Self-efficacy and quality of life after low-intensity neuropsychological rehabilitation: A pre-post intervention study
Authors: Brands Ingrid , Custers Maud , van Heugten Caroline
Published: February 2017

BACKGROUND: Being highly self-efficacious is a key factor in successful chronic disease self-management. It is unknown whether neuropsychological rehabilitation improves self-efficacy in managing the consequences of brain injury.

OBJECTIVE: To investigate whether levels of general and brain injury specific self-efficacy and quality of life (QoL) increased after neuropsychological rehabilitation and whether cognitive performance was associated with self-efficacy.

METHODS: We conducted a retrospective clinical cohort study of 62 patients with acquired brain injury and cognitive complaints with measurements before start and after completion of treatment. QoL was measured with the visual analogue scale (EQ VAS) of the EuroQol (EQ-5D); self-efficacy with the TBI Self-efficacy Questionnaire (SEsx) and the General Self-efficacy Scale (GSES). Cognitive performance was measured as a compound score of tests for memory, attention and information processing speed.

RESULTS: Self-efficacy for managing brain injury-specific symptoms and QoL increased significantly after neuropsychological rehabilitation. Both general and brain injury-specific self-efficacy were positively associated with QoL after completion of the programme. Cognitive performance was not associated with self-efficacy for managing brain injury-specific symptoms nor with general self-efficacy.

CONCLUSIONS: Self-efficacy and QoL improve after treatment. Further research is needed to identify the specific ingredients responsible for improvement of self-efficacy in patients with cognitive complaints.
Analysis and Improvement of Fireworks Algorithm
Authors: Li Xi-Guang , Han Shou-Fei , Gong Chang-Qing
Published: February 2017

The Fireworks Algorithm is a recently developed swarm intelligence algorithm to simulate the explosion process of fireworks. Based on the analysis of each operator of Fireworks Algorithm (FWA), this paper improves the FWA and proves that the improved algorithm converges to the global optimal solution with probability 1. The proposed algorithm improves the goal of further boosting performance and achieving global optimization where mainly include the following strategies. Firstly using the opposition-based learning initialization population. Secondly a new explosion amplitude mechanism for the optimal firework is proposed. In addition, the adaptive t-distribution mutation for non-optimal individuals and elite opposition-based learning for the optimal individual are used. Finally, a new selection strategy, namely Disruptive Selection, is proposed to reduce the running time of the algorithm compared with FWA. In our simulation, we apply the CEC2013 standard functions and compare the proposed algorithm (IFWA) with SPSO2011, FWA, EFWA and dynFWA. The results show that the proposed algorithm has better overall performance on the test functions.
Fragile Watermarking for Image Authentication Using the Characteristic of SVD
Authors: Zhang Heng , Wang Chengyou , Zhou Xiao
Published: February 2017

Digital image authentication has become a hot topic in the last few years. In this paper, a pixel-based fragile watermarking method is presented for image tamper identification and localization. By analyzing the left and right singular matrices of SVD, it is found that the matrix product between the first column of the left singular matrix and the transposition of the first column in the right singular matrix is closely related to the image texture features. Based on this characteristic, a binary watermark consisting of image texture information is generated and inserted into the least significant bit (LSB) of the original host image. To improve the security of the presented algorithm, the Arnold transform is applied twice in the watermark embedding process. Experimental results indicate that the proposed watermarking algorithm has high security and perceptual invisibility. Moreover, it can detect and locate the tampered region effectively for various malicious attacks.
Surface Quality of Staggered PCD End Mill in Milling of Carbon Fiber Reinforced Plastics
Authors: Liu Guangjun , Chen Hongyuan , Huang Zhen , Gao Fei , Chen Tao
Published: February 2017

Machined surface quality determines the reliability, wear resistance and service life of carbon fiber reinforced plastic (CFRP) workpieces. In this work, the formation mechanism of the surface topography and the machining defects of CFRPs are proposed, and the influence of milling parameters and fiber cutting angles on the surface quality of CFRPs is obtained, which can provide a reference for extended tool life and good surface quality. Trimming and slot milling tests of unidirectional CFRP laminates are performed. The surface roughness of the machined surface is measured, and the influence of milling parameters on the surface roughness is analyzed. A regression model for the surface roughness of CFRP milling is established. A significance test of the regression model is conducted. The machined surface topography of milling CFRP unidirectional laminates with different fiber orientations is analyzed, and the effect of fiber cutting angle on the surface topography of the machined surface is presented by using a digital super depth-of-field microscope and scanning electron microscope (SEM). To study the influence of fiber cutting angle on machining defects, the machined topography under different fiber orientations is analyzed. The slot milling defects and their formation mechanism under different fiber cutting angles are investigated.
Numerical Study on the Asphalt Concrete Structure for Blast and Impact Load Using the Karagozian and Case Concrete Model
Authors: Wu Jun , Li Liang , Du Xiuli , Liu Xuemei
Published: February 2017

The behaviour of an asphalt concrete structure subjected to severe loading, such as blast and impact loadings, is becoming critical for safety and anti-terrorist reasons. With the development of high-speed computational capabilities, it is possible to carry out the numerical simulation of an asphalt concrete structure subjected to blast or impact loading. In the simulation, the constitutive model plays a key role as the model defines the essential physical mechanisms of the material under different stress and loading conditions. In this paper, the key features of the Karagozian and Case concrete model (KCC) adopted in LSDYNA are evaluated and discussed. The formulations of the strength surfaces and the damage factor in the KCC model are verified. Both static and dynamic tests are used to determine the parameters of asphalt concrete in the KCC model. The modified damage factor is proposed to represent the higher failure strain that can improve the simulation of the behaviour of AC material. Furthermore, a series test of the asphalt concrete structure subjected to blast and impact loadings is conducted and simulated by using the KCC model. The simulation results are then compared with those from both field and laboratory tests. The results show that the use of the KCC model to simulate asphalt concrete structures can reproduce similar results as the field and laboratory test.
Mechanisms of Chromosome Congression during Mitosis
Authors: Maiato Helder , Gomes Ana , Sousa Filipe , Barisic Marin
Published: February 2017

Chromosome congression during prometaphase culminates with the establishment of a metaphase plate, a hallmark of mitosis in metazoans. Classical views resulting from more than 100 years of research on this topic have attempted to explain chromosome congression based on the balance between opposing pulling and/or pushing forces that reach an equilibrium near the spindle equator. However, in mammalian cells, chromosome bi-orientation and force balance at kinetochores are not required for chromosome congression, whereas the mechanisms of chromosome congression are not necessarily involved in the maintenance of chromosome alignment after congression. Thus, chromosome congression and maintenance of alignment are determined by different principles. Moreover, it is now clear that not all chromosomes use the same mechanism for congressing to the spindle equator. Those chromosomes that are favorably positioned between both poles when the nuclear envelope breaks down use the so-called “direct congression” pathway in which chromosomes align after bi-orientation and the establishment of end-on kinetochore-microtubule attachments. This favors the balanced action of kinetochore pulling forces and polar ejection forces along chromosome arms that drive chromosome oscillatory movements during and after congression. The other pathway, which we call “peripheral congression”, is independent of end-on kinetochore microtubule-attachments and relies on the dominant and coordinated action of the kinetochore motors Dynein and Centromere Protein E (CENP-E) that mediate the lateral transport of peripheral chromosomes along microtubules, first towards the poles and subsequently towards the equator. How the opposite polarities of kinetochore motors are regulated in space and time to drive congression of peripheral chromosomes only now starts to be understood. This appears to be regulated by position-dependent phosphorylation of both Dynein and CENP-E and by spindle microtubule diversity by means of tubulin post-translational modifications. This so-called “tubulin code” might work as a navigation system that selectively guides kinetochore motors with opposite polarities along specific spindle microtubule populations, ultimately leading to the congression of peripheral chromosomes. We propose an integrated model of chromosome congression in mammalian cells that depends essentially on the following parameters: (1) chromosome position relative to the spindle poles after nuclear envelope breakdown; (2) establishment of stable end-on kinetochore-microtubule attachments and bi-orientation; (3) coordination between kinetochore- and arm-associated motors; and (4) spatial signatures associated with post-translational modifications of specific spindle microtubule populations. The physiological consequences of abnormal chromosome congression, as well as the therapeutic potential of inhibiting chromosome congression are also discussed.
MetaGaAP: A Novel Pipeline to Estimate Community Composition and Abundance from Non-Model Sequence Data
Authors: Noune Christopher , Hauxwell Caroline
Published: February 2017

Next generation sequencing and bioinformatic approaches are increasingly used to quantify microorganisms within populations by analysis of ‘meta-barcode’ data. This approach relies on comparison of amplicon sequences of ‘barcode’ regions from a population with public-domain databases of reference sequences. However, for many organisms relevant ‘barcode’ regions may not have been identified and large databases of reference sequences may not be available. A workflow and software pipeline, ‘MetaGaAP,’ was developed to identify and quantify genotypes through four steps: shotgun sequencing and identification of polymorphisms in a metapopulation to identify custom ‘barcode’ regions of less than 30 polymorphisms within the span of a single ‘read’, amplification and sequencing of the ‘barcode’, generation of a custom database of polymorphisms, and quantitation of the relative abundance of genotypes. The pipeline and workflow were validated in a ‘wild type’ Alphabaculovirus isolate, Helicoverpa armigera single nucleopolyhedrovirus (HaSNPV-AC53) and a tissue-culture derived strain (HaSNPV-AC53-T2). The approach was validated by comparison of polymorphisms in amplicons and shotgun data, and by comparison of predicted dominant and co-dominant genotypes with Sanger sequences. The computational power required to generate and search the database effectively limits the number of polymorphisms that can be included in a barcode to 30 or less. The approach can be used in quantitative analysis of the ecology and pathology of non-model organisms.
Designing the Sniper: Improving Targeted Human Cytolytic Fusion Proteins for Anti-Cancer Therapy via Molecular Simulation
Authors: Bochicchio Anna , Jordaan Sandra , Losasso Valeria , Chetty Shivan , Perera Rodrigo , Ippoliti Emiliano , Barth Stefan , Carloni Paolo
Published: February 2017

Targeted human cytolytic fusion proteins (hCFPs) are humanized immunotoxins for selective treatment of different diseases including cancer. They are composed of a ligand specifically binding to target cells genetically linked to a human apoptosis-inducing enzyme. hCFPs target cancer cells via an antibody or derivative (scFv) specifically binding to e.g., tumor associated antigens (TAAs). After internalization and translocation of the enzyme from endocytosed endosomes, the human enzymes introduced into the cytosol are efficiently inducing apoptosis. Under in vivo conditions such enzymes are subject to tight regulation by native inhibitors in order to prevent inappropriate induction of cell death in healthy cells. Tumor cells are known to upregulate these inhibitors as a survival mechanism resulting in escape of malignant cells from elimination by immune effector cells. Cytosolic inhibitors of Granzyme B and Angiogenin (Serpin P9 and RNH1, respectively), reduce the efficacy of hCFPs with these enzymes as effector domains, requiring detrimentally high doses in order to saturate inhibitor binding and rescue cytolytic activity. Variants of Granzyme B and Angiogenin might feature reduced affinity for their respective inhibitors, while retaining or even enhancing their catalytic activity. A powerful tool to design hCFPs mutants with improved potency is given by in silico methods. These include molecular dynamics (MD) simulations and enhanced sampling methods (ESM). MD and ESM allow predicting the enzyme-protein inhibitor binding stability and the associated conformational changes, provided that structural information is available. Such “high-resolution” detailed description enables the elucidation of interaction domains and the identification of sites where particular point mutations may modify those interactions. This review discusses recent advances in the use of MD and ESM for hCFP development from the viewpoints of scientists involved in both fields.
Assessment on the Effects of ZnO and Coated ZnO Particles on iPP and PLA Properties for Application in Food Packaging
Authors: Marra Antonella , Rollo Gennaro , Cimmino Sossio , Silvestre Clara
Published: February 2017

This paper compares the properties of iPP based composites and PLA based biocomposites using 5% of ZnO particles or ZnO particles coated with stearic acid as filler. In particular, the effect of coating on the UV stability, thermostability, mechanical, barrier, and antibacterial properties of the polymer matrix were compared and related to the dispersion and distribution of the loads in the polymer matrix and the strength of the adhesion between the matrix and the particles. This survey demonstrated that, among the reported systems, iPP/5%ZnOc and PLA/5%ZnO films are the most suitable active materials for potential application in the active food packaging field.
Anisotropic Magnetic Responses of Topological Crystalline Superconductors
Authors: Xiong Yuansen , Yamakage Ai , Kobayashi Shingo , Sato Masatoshi , Tanaka Yukio
Published: February 2017

Majorana Kramers pairs emerged on surfaces of time-reversal-invariant topological crystalline superconductors show the Ising anisotropy to an applied magnetic ﬁeld. We clarify that crystalline symmetry uniquely determines the direction of the Majorana Ising spin for given irreduciblerepresentationsofpairpotential,derivingconstraintstotopologicalinvariants. In addition, necessary conditions for nontrivial topological invariants protected by the n-fold rotational symmetry are shown.
Diversity of the Mountain Flora of Central Asia with Emphasis on Alkaloid-Producing Plants
Authors: Tayjanov Karimjan , Mamadalieva Nilufar , Wink Michael
Published: February 2017

The mountains of Central Asia with 70 large and small mountain ranges represent species-rich plant biodiversity hotspots. Major mountains include Saur, Tarbagatai, Dzungarian Alatau, Tien Shan, Pamir-Alai and Kopet Dag. Because a range of altitudinal belts exists, the region is characterized by high biological diversity at ecosystem, species and population levels. In addition, the contact between Asian and Mediterranean flora in Central Asia has created unique plant communities. More than 8100 plant species have been recorded for the territory of Central Asia; about 5000–6000 of them grow in the mountains. The aim of this review is to summarize all the available data from 1930 to date on alkaloid-containing plants of the Central Asian mountains. In Saur 301 of a total of 661 species, in Tarbagatai 487 out of 1195, in Dzungarian Alatau 699 out of 1080, in Tien Shan 1177 out of 3251, in Pamir-Alai 1165 out of 3422 and in Kopet Dag 438 out of 1942 species produce alkaloids. The review also tabulates the individual alkaloids which were detected in the plants from the Central Asian mountains. Quite a large number of the mountain plants produce neurotoxic and cytotoxic alkaloids, indicating that a strong chemical defense is needed under the adverse environmental conditions of these mountains with presumably high pressure from herbivores.
An Optimized Energy Management Strategy for Preheating Vehicle-Mounted Li-ion Batteries at Subzero Temperatures
Authors: Zhu Tao , Min Haitao , Yu Yuanbin , Zhao Zhongmin , Xu Tao , Chen Yang , Li Xinyong , Zhang Cong
Published: February 2017

This paper presents an optimized energy management strategy for Li-ion power batteries used on electric vehicles (EVs) at low temperatures. In low-temperature environments, EVs suffer a sharp driving range loss resulting from the energy and power capability reduction of the battery. Simultaneously, because of Li plating, battery degradation becomes an increasing concern as the temperature drops. All these factors could greatly increase the total vehicle operation cost. Prior to battery charging and vehicle operating, preheating the battery to a battery-friendly temperature is an approach to promote energy utilization and reduce total cost. Based on the proposed LiFePO4 battery model, the total vehicle operation cost under certain driving cycles is quantified in the present paper. Then, given a certain ambient temperature, a target preheating temperature is optimized under the principle of minimizing total cost. As for the preheating method, a liquid heating system is also implemented on an electric bus. Simulation results show that the preheating process becomes increasingly necessary with decreasing ambient temperature, however, the preheating demand declines as driving range grows. Vehicle tests verify that the preheating management strategy proposed in this paper is able to save on total vehicle operation costs.
The Ageing Brain: Effects on DNA Repair and DNA Methylation in Mice
Authors: Langie Sabine , Cameron Kerry , Ficz Gabriella , Oxley David , Tomaszewski Bartłomiej , Gorniak Joanna , Maas Lou , Godschalk Roger , Van Schooten Frederik , Reik Wolf , Von Zglinicki Thomas , Mathers John
Published: February 2017

Base excision repair (BER) may become less effective with ageing resulting in accumulation of DNA lesions, genome instability and altered gene expression that contribute to age-related degenerative diseases. The brain is particularly vulnerable to the accumulation of DNA lesions; hence, proper functioning of DNA repair mechanisms is important for neuronal survival. Although the mechanism of age-related decline in DNA repair capacity is unknown, growing evidence suggests that epigenetic events (e.g., DNA methylation) contribute to the ageing process and may be functionally important through the regulation of the expression of DNA repair genes. We hypothesize that epigenetic mechanisms are involved in mediating the age-related decline in BER in the brain. Brains from male mice were isolated at 3–32 months of age. Pyrosequencing analyses revealed significantly increased Ogg1 methylation with ageing, which correlated inversely with Ogg1 expression. The reduced Ogg1 expression correlated with enhanced expression of methyl-CpG binding protein 2 and ten-eleven translocation enzyme 2. A significant inverse correlation between Neil1 methylation at CpG-site2 and expression was also observed. BER activity was significantly reduced and associated with increased 8-oxo-7,8-dihydro-2′-deoxyguanosine levels. These data indicate that Ogg1 and Neil1 expression can be epigenetically regulated, which may mediate the effects of ageing on DNA repair in the brain.
Early Insights from Commercialization of Gene Therapies in Europe
Authors: Touchot Nicolas , Flume Mathias
Published: February 2017

After years of research and development, gene therapies are now becoming a commercial reality with several products approved by European regulatory authorities [...]
Determinants of Health Care Services Utilization among First Generation Afghan Migrants in Istanbul
Authors: Alemi Qais , Stempel Carl , Koga Patrick , Smith Valerie , Danis Didem , Baek Kelly , Montgomery Susanne
Published: February 2017

There is insufficient empirical evidence on the correlates of health care utilization of irregular migrants currently living in Turkey. The aim of this study was to identify individual level determinants associated with health service and medication use. One hundred and fifty-five Afghans completed surveys assessing service utilization including encounters with primary care physicians and outpatient specialists in addition to the use of prescription and nonprescription medicines. Multivariate logistic regression analyses were employed to examine associations between service use and a range of predisposing, enabling, and perceived need factors. Health services utilization was lowest for outpatient specialists (20%) and highest for nonprescription medications (37%). Female gender and higher income predicted encounters with primary care physicians. Income, and other enabling factors such as family presence in Turkey predicted encounters with outpatient specialists. Perceived illness-related need factors had little to no influence on use of services; however, asylum difficulties increased the likelihood for encounters with primary care physicians, outpatient services, and the use of prescription medications. This study suggests that health services use among Afghan migrants in Turkey is low considering the extent of their perceived illness-related needs, which may be further exacerbated by the precarious conditions in which they live.
A Formal Framework for Integrated Environment Modeling Systems
Authors: Zhang Gaofeng , Li Yan , Chen Chong , Zhou Rui , Chen Dan , Zhou Qingguo
Published: February 2017

Integrated Environment Modeling (IEM) has become more and more important for environmental studies and applications. IEM systems have also been extended from scientific studies to much wider practical application situations. The quality and improved efficiency of IEM systems have therefore become increasingly critical. Although many advanced and creative technologies have been adopted to improve the quality of IEM systems, there is scarcely any formal method for evaluating and improving them. This paper is devoted to proposing a formal method to improve the quality and the developing efficiency of IEM systems. Two primary contributions are made. Firstly, a formal framework for IEM is proposed. The framework not only reflects the static and dynamic features of IEM but also covers different views from variant roles throughout the IEM lifecycle. Secondly, the formal operational semantics corresponding to the former model of the IEM is derived in detail; it can be used as the basis for aiding automated integrated modeling and verifying the integrated model.
Proteomic Analysis of Mitochondria-Enriched Fraction Isolated from the Frontal Cortex and Hippocampus of Apolipoprotein E Knockout Mice Treated with Alda-1, an Activator of Mitochondrial Aldehyde Dehydrogenase (ALDH2)
Authors: Stachowicz Aneta , Olszanecki Rafał , Suski Maciej , Głombik Katarzyna , Basta-Kaim Agnieszka , Adamek Dariusz , Korbut Ryszard
Published: February 2017

The role of different genotypes of apolipoprotein E (apoE) in the etiology of Alzheimer’s disease is widely recognized. It has been shown that altered functioning of apoE may promote 4-hydroxynonenal modification of mitochondrial proteins, which may result in mitochondrial dysfunction, aggravation of oxidative stress, and neurodegeneration. Mitochondrial aldehyde dehydrogenase (ALDH2) is an enzyme considered to perform protective function in mitochondria by the detoxification of the end products of lipid peroxidation, such as 4-hydroxynonenal and other reactive aldehydes. The goal of our study was to apply a differential proteomics approach in concert with molecular and morphological techniques to elucidate the changes in the frontal cortex and hippocampus of apolipoprotein E knockout (apoE−/−) mice upon treatment with Alda-1—a small molecular weight activator of ALDH2. Despite the lack of significant morphological changes in the brain of apoE−/− mice as compared to age-matched wild type animals, the proteomic and molecular approach revealed many changes in the expression of genes and proteins, indicating the impairment of energy metabolism, neuroplasticity, and neurogenesis in brains of apoE−/− mice. Importantly, prolonged treatment of apoE−/− mice with Alda-1 led to the beneficial changes in the expression of genes and proteins related to neuroplasticity and mitochondrial function. The pattern of alterations implies mitoprotective action of Alda-1, however, the accurate functional consequences of the revealed changes require further research.
Determination of VEGFR-2 (KDR) 604A>G Polymorphism in Pancreatic Disorders
Authors: Pădureanu Vlad , Boldeanu Mihail , Streaţă Ioana , Cucu Mihai , Siloşi Isabela , Boldeanu Lidia , Bogdan Maria , Enescu Anca , Forţofoiu Maria , Enescu Aurelia , Dumitrescu Elena , Alexandru Dragoş , Şurlin Valeriu , Forţofoiu Mircea , Petrescu Ileana , Petrescu Florin , Ioana Mihai , Ciurea Marius , Săftoiu Adrian
Published: February 2017

Pancreatic disorders have a high prevalence worldwide. Despite the fact that screening methods became more effective and the knowledge we have nowadays about pancreatic diseases has enhanced, their incidence remains high. Our purpose was to determine whether single nucleotide polymorphism (SNP) of VEGFR-2/KDR (vascular endothelial growth factor receptor 2/kinase insert domain receptor) influences susceptibility to develop pancreatic pathology. Genomic DNA was extracted from blood samples collected from patients diagnosed with acute pancreatitis (n = 110), chronic pancreatitis (n = 25), pancreatic cancer (n = 82) and healthy controls (n = 232). VEGFR-2 (KDR) 604A>G (rs2071559) polymorphism frequency was determined with TaqMan allelic discrimination assays. Statistical assessment was performed by associating genetic polymorphism with clinical and pathological data. In both pancreatic disorders and healthy control groups the polymorphism we studied was in Hardy-Weinberg equilibrium. Association between increased risk for pancreatic disorders and studied polymorphism was statistically significant. KDR 604AG and AG + GG genotypes were more prevalent in acute pancreatitis and pancreatic cancer patients than in controls. These genotypes influence disease development in a low rate. No association was found between chronic pancreatitis and KDR 604AG and AG + GG genotypes. In Romanian cohort, we found an association between the KDR 604A→G polymorphism and acute pancreatitis and pancreatic cancer. Carriers of the -604G variant allele were more frequent among acute pancreatitis and pancreatic cancer than among controls, suggesting that KDR 604G allele may confer an increased risk for these diseases. In the future, more extensive studies on larger groups are necessary, in order to clarify the role of VEGFR2 polymorphisms in pancreatic pathology.
Matrix Metalloproteinases as Regulators of Periodontal Inflammation
Authors: Franco Cavalla , Patricia Hernández-Ríos , Timo Sorsa , Claudia Biguetti , Marcela Hernández
Published: February 2017

Periodontitis are infectious diseases characterized by immune-mediated destruction of periodontal supporting tissues and tooth loss. Matrix metalloproteinases (MMPs) are key proteases involved in destructive periodontal diseases. The study and interest in MMP has been fuelled by emerging evidence demonstrating the broad spectrum of molecules that can be cleaved by them and the myriad of biological processes that they can potentially regulate. The huge complexity of MMP functions within the ‘protease web’ is crucial for many physiologic and pathologic processes, including immunity, inflammation, bone resorption, and wound healing. Evidence points out that MMPs assemble in activation cascades and besides their classical extracellular matrix substrates, they cleave several signalling molecules—such as cytokines, chemokines, and growth factors, among others—regulating their biological functions and/or bioavailability during periodontal diseases. In this review, we provide an overview of emerging evidence of MMPs as regulators of periodontal inflammation.
Impact of Consuming ‘Toxic’ Monarch Caterpillars on Adult Chinese Mantid Mass Gain and Fecundity
Authors: Rafter Jamie , Gonda-King Liahna , Niesen Daniel , Seeram Navindra , Rigsby Chad , Preisser Evan
Published: February 2017

Predators that feed on chemically-defended prey often experience non-lethal effects that result in learned avoidance of the prey species. Some predators are able to consume toxic prey without ill-effect. The Chinese mantid is able to consume cardenolide-containing monarch caterpillars without immediate adverse effects. Although they discard the caterpillars’ gut contents, mantids consume sequestered cardenolides. Although consumption of these cardenolides does not elicit an acute response, there may be long-term costs associated with cardenolide consumption. We tested the hypothesis that consumption of monarch caterpillars will adversely affect adult mantid biomass gain and reproductive condition. We reared mantids from egg to adult and assigned them to one of four toxicity groups that differed in the number of monarch caterpillars offered over a 15-day period. Mantids consumed similar amounts of prey biomass during the experiment. Yet, mantids in the high-toxicity group had a higher conversion efficiency and gained more biomass than mantids in other groups. Mantids in all treatment groups produced similar numbers of eggs. However, mantids in the high-toxicity group produced heavier eggs and devoted a greater portion of their biomass toward egg production than those in the control group. This increase in reproductive condition is probably driven by variation in prey nutritional value and/or the nutritional advantages inherent in eating multiple food types. Our results demonstrate the mantid is able to incorporate ‘toxic’ monarch prey into its diet without acute or chronic ill-effects.
Progress in Tribological Properties of Nano-Composite Hard Coatings under Water Lubrication
Authors: Wang Qianzhi , Zhou Fei
Published: February 2017

The tribological properties, under water-lubricated conditions, of three major nano-composite coatings, i.e., diamond-like carbon (DLC or a-C), amorphous carbon nitride (a-CNx) and transition metallic nitride-based (TiN-based, CrN-based), coatings are reviewed. The influences of microstructure (composition and architecture) and test conditions (counterparts and friction parameters) on their friction and wear behavior under water lubrication are systematically elucidated. In general, DLC and a-CNx coatings exhibit superior tribological performance under water lubrication due to the formation of the hydrophilic group and the lubricating layer with low shear strength, respectively. In contrast, TiN-based and CrN-based coatings present relatively poor tribological performance in pure water, but are expected to present promising applications in sea water because of their good corrosion resistance. No matter what kind of coatings, an appropriate selection of counterpart materials would make their water-lubricated tribological properties more prominent. Currently, Si-based materials are deemed as beneficial counterparts under water lubrication due to the formation of silica gel originating from the hydration of Si. In the meantime, the tribological properties of nano-composite coatings in water could be enhanced at appropriate normal load and sliding velocity due to mixed or hydrodynamic lubrication. At the end of this article, the main research that is now being developed concerning the development of nano-composite coatings under water lubrication is described synthetically.
The Effect of Matrix Composition on the Deformation and Failure Mechanisms in Metal Matrix Syntactic Foams during Compression
Authors: Kádár Csilla , Máthis Kristián , Knapek Michal , Chmelík František
Published: February 2017

The influence of the matrix material on the deformation and failure mechanisms in metal matrix syntactic foams was investigated in this study. Samples with commercially pure Al (Al) and Al-12 wt % Si (AlSi12) eutectic aluminum matrix, reinforced by hollow ceramic spheres, were compressed at room temperature. Concurrently, the acoustic emission response and the strain field development on the surface were monitored in-situ. The results indicate that the plastic deformation of the cell walls is the governing mechanism in the early stage of straining for both types of foams. At large stresses, deformation bands form both in the Al and AlSi12 foam. In Al foam, cell walls collapse in a large volume. In contrast, the AlSi12 foam is more brittle; therefore, the fracture of precipitates and the crushing of the matrix take place within a distinctive deformation band, along with an occurrence of a significant stress drop. The onset stress of ceramic sphere failure was shown to be not influenced by the matrix material. The in-situ methods provided complementary data which further support these results.
HRTEM Microstructural Characterization of β-WO3 Thin Films Deposited by Reactive RF Magnetron Sputtering
Authors: Faudoa-Arzate A. , Arteaga-Durán A. , Saenz-Hernández R.J. , Botello-Zubiate M.E. , Realyvazquez-Guevara P.R. , Matutes-Aquino J.A.
Published: February 2017

Though tungsten trioxide (WO3) in bulk, nanosphere, and thin film samples has been extensively studied, few studies have been dedicated to the crystallographic structure of WO3 thin films. In this work, the evolution from amorphous WO3 thin films to crystalline WO3 thin films is discussed. WO3 thin films were fabricated on silicon substrates (Si/SiO2) by RF reactive magnetron sputtering. Once a thin film was deposited, two successive annealing treatments were made: an initial annealing at 400 °C for 6 h was followed by a second annealing at 350 °C for 1 h. Film characterization was carried out by X-ray diffraction (XRD), high-resolution electron transmission microscopy (HRTEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM) techniques. The β-WO3 final phase grew in form of columnar crystals and its growth plane was determined by HRTEM.
Briarane Diterpenoids Isolated from Octocorals between 2014 and 2016
Authors: Su Yin-Di , Su Jui-Hsin , Hwang Tsong-Long , Wen Zhi-Hong , Sheu Jyh-Horng , Wu Yang-Chang , Sung Ping-Jyun
Published: February 2017

The structures, names, bioactivities, and references of 124 briarane-type natural products, including 66 new metabolites, isolated between 2014 and 2016 are summarized in this review article. All of the briarane diterpenoids mentioned in this review were isolated from octocorals, mainly from Briareum violacea, Dichotella gemmacea, Ellisella dollfusi, Junceella fragilis, Junceella gemmacea, and Pennatula aculeata. Some of these compounds exhibited potential biomedical activities, including anti-inflammatory activity, antibacterial activity, and cytotoxicity towards cancer cells.
Review of Membranes for Helium Separation and Purification
Authors: Scholes Colin , Ghosh Ujjal
Published: February 2017

Membrane gas separation has potential for the recovery and purification of helium, because the majority of membranes have selectivity for helium. This review reports on the current state of the research and patent literature for membranes undertaking helium separation. This includes direct recovery from natural gas, as an ancillary stage in natural gas processing, as well as niche applications where helium recycling has potential. A review of the available polymeric and inorganic membranes for helium separation is provided. Commercial gas separation membranes in comparable gas industries are discussed in terms of their potential in helium separation. Also presented are the various membrane process designs patented for the recovery and purification of helium from various sources, as these demonstrate that it is viable to separate helium through currently available polymeric membranes. This review places a particular focus on those processes where membranes are combined in series with another separation technology, commonly pressure swing adsorption. These combined processes have the most potential for membranes to produce a high purity helium product. The review demonstrates that membrane gas separation is technically feasible for helium recovery and purification, though membranes are currently only applied in niche applications focused on reusing helium rather than separation from natural sources.
Expression, Purification, and Monitoring of Conformational Changes of hCB2 TMH67H8 in Different Membrane-Mimetic Lipid Mixtures Using Circular Dichroism and NMR Techniques
Authors: Tiburu Elvis , Zhuang Jianqin , Fleischer Heidimarie , Arthur Patrick , Awandare Gordon
Published: February 2017

This work was intended to develop self-assembly lipids for incorporating G-protein coupled receptors (GPCRs) in order to improve the success rate for nuclear magnetic resonance spectroscopy (NMR) structural elucidation. We hereby report the expression and purification of uniformly 15N-labeled human cannabinoid receptor-2 domain in insect cell media. The domain was refolded by screening several membrane mimetic environments. Different q ratios of isotropic bicelles were screened for solubilizing transmembrane helix 6, 7 and 8 (TMH67H8). As the concentration of dimyristoylphosphocholine (DMPC) was increased such that the q ratio was between 0.16 and 0.42, there was less crowding in the cross peaks with increasing q ratio. In bicelles of q = 0.42, the maximum number of cross peaks were obtained and the cross peaks were uniformly dispersed. The receptor domain in bicelles beyond q = 0.42 resulted in peak crowding. These studies demonstrate that GPCRs folding especially in bicelles is protein-specific and requires the right mix of the longer chain and shorter chain lipids to provide the right environment for proper folding. These findings will allow further development of novel membrane mimetics to provide greater diversity of lipid mixtures than those currently being employed for GPCR stability and folding, which are critical for both X-ray and NMR studies of GPCRs.
Visible to Infrared Diamond Photonics Enabled by Focused Femtosecond Laser Pulses
Authors: Sotillo Belén , Bharadwaj Vibhav , Hadden John , Rampini Stefano , Chiappini Andrea , Fernandez Toney , Armellini Cristina , Serpengüzel Ali , Ferrari Maurizio , Barclay Paul , Ramponi Roberta , Eaton Shane
Published: February 2017

Diamond’s nitrogen-vacancy (NV) centers show great promise in sensing applications and quantum computing due to their long electron spin coherence time and because they can be found, manipulated, and read out optically. An important step forward for diamond photonics would be connecting multiple diamond NVs together using optical waveguides. However, the inertness of diamond is a significant hurdle for the fabrication of integrated optics similar to those that revolutionized silicon photonics. In this work, we show the fabrication of optical waveguides in diamond, enabled by focused femtosecond high repetition rate laser pulses. By optimizing the geometry of the waveguide, we obtain single mode waveguides from the visible to the infrared. Additionally, we show the laser writing of individual NV centers within the bulk of diamond. We use µ-Raman spectroscopy to gain better insight on the stress and the refractive index profile of the optical waveguides. Using optically detected magnetic resonance and confocal photoluminescence characterization, high quality NV properties are observed in waveguides formed in various grades of diamond, making them promising for applications such as magnetometry, quantum information systems, and evanescent field sensors.