bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Fatty Acid Synthase in Arma chinensis and Its Expression Profiles in Response to Starvation
Authors: Ma Le , Cheng Hongmei , Lin Changjin , Fu Luyao , Liu Dianyu , Chen Yu , Su Zhihan , Yan Xiaoyu , Xu Wenyan , Dong Xiaolin , Liu Chenxi
Published: February 2025

Fatty acids are the main form of energy production and storage in insects, and fatty acid synthase (FAS) plays a major role in this process. Arma chinensis is a natural enemy used in China to control agricultural pests. While its whole genome has been assembled, its FAS genes remain undescribed. Herein, we aim to identify the FAS genes of A. chinensis and explore their expression during starvation. Phylogenetic analysis identified nine FAS genes (ArmaFas1–ArmaFas9). During the predatory developmental stages, ArmaFas9 expression gradually increased from the 2nd to the 4th instar, peaking at the 5th instar and plateauing at the adult stage. Female adults exhibited the highest expression of ArmaFas9 in their fat body and the lowest expression in their salivary glands, whereas male adults demonstrated the opposite pattern. ArmaFas9 expression was negatively correlated with starvation duration. Compared to the controls, starved adult females exhibited a downward expression trend, whereas starved adult males demonstrated an initial increase, followed by a downward trend after 7 days. Starvation and refeeding increased ArmaFas9 expression. These results demonstrate the important role of ArmaFas9 in A. chinensis growth and development, providing a basis for further studies on lipid synthesis in predatory bugs.
Neem and Gliricidia Plant Leaf Extracts Improve Yield and Quality of Leaf Mustard by Managing Insect Pests’ Abundance Without Harming Beneficial Insects and Some Sensory Attributes
Authors: Kamanga Rowland Maganizo , Bhikha Salifu , Kamala Felix Dalitso , Mwale Vincent Mgoli , Tembo Yolice , Ndakidemi Patrick Alois
Published: February 2025

Production and consumption of vegetable crops has seen a sharp increase in the recent past owing to an increasing recognition of their nutraceutical benefits. In tandem, there has been unwarranted application of agrochemicals such as insecticides to enhance productivity and vegetable quality, at the cost of human health, and fundamental environmental and ecosystem functions and services. This study was conducted to evaluate the efficacy of neem and gliricidia botanical extracts in managing harmful insect pest populations in leaf mustard. Our results report that neem and gliricidia plant extracts enhance the yield and quality of leaf mustard by reducing the prevalence and feeding activity of harmful insect pests in a manner similar to synthetic insecticides. Some of the key insect pests reduced were Lipaphis erysimi, Pieris oleracea, Phyllotreta Cruciferae, Melanoplus sanguinipes, and Murgantia histrionica. However, compared to synthetic insecticides, neem and gliricidia plant extracts were able to preserve beneficial insects such as the Coccinellidae spp., Trichogramma minutum, Araneae spp., Lepidoptera spp., and Blattodea spp. Furthermore, plant extracts did not significantly alter sensory attributes, especially taste and odor, whereas the visual appearance of leaf mustard was greater in plants sprayed with neem and synthetic insecticides. Physiologically, plant extracts were also able to significantly lower leaf membrane damage as shown through the electrolyte leakage assay. Therefore, these plant extracts represent promising pesticidal plant materials and botanically active substances that can be leveraged to develop environmentally friendly commercial pest management products.
Robust Control of Irrigation Systems Using Predictive Methods and Disturbance Rejection
Authors: Carreño-Zagarra Jose , Poveda-Rodriguez Diana , Flórez Marco
Published: February 2025

Ensuring that the world’s population meets its food needs, despite water restrictions, can be significantly improved by increasing irrigation efficiency and productivity. Achieving this goal necessitates technological advancements in control systems. Therefore, the implementation of effective control systems across the entire irrigation water distribution chain is crucial and requires technological modernization. This paper presents a control scheme that combines the benefits of model predictive control (MPC) and active disturbance rejection by using generalized proportional integral (GPI) observers. The proposed control scheme was applied to a three-canal irrigation system. The simulation results confirm that the proposed controller is robust to disturbances and ensures accurate tracking for all reference levels. The controller’s performance is highlighted by the improvement in response time and considerable reduction in overshoot compared with the optimized proportional integral (PI) controllers. Additionally, the use of GPI observers allows for the precise estimation of nonlinear disturbances and phase variables, enhancing the robustness of the system. The efficiency of the observer is due to its ability to adequately estimate global additive disturbances, including unknown parameters and external disturbances in the input–output dynamics.
Optimization of 3D Printing Parameters for Enhanced Mechanical Strength: Effects of Glass Fiber Reinforcement and Fill Ratio Using RSM and ANOVA
Authors: Jawad Hussein Hadi , Kordani Naser , Bagheri Abbasali , Derazkola Hamed Aghajani
Published: February 2025

This research aimed to provide valuable insights for future studies and enhance manufacturing processes by investigating the effect of incorporating fibers into 3D printing to improve the mechanical properties of fabricated components. The experimental design was carried out using Design-Expert software, employing the Central Composite Design (CCD) methodology. Seventeen experiments were conducted, with predefined input parameters, layer height, filler ratio, and printing speed, analyzed through the Response Surface Methodology (RSM) using Design-Expert version 12. An Analysis of Variance (ANOVA) revealed that the filler ratio had the most significant effect on fracture strength. The influence of different printing parameters printing speed, layer height, and filler ratio on the mechanical properties and print quality was systematically investigated. The results indicated that the filler ratio was the most critical factor, with a 100% fill ratio yielding the highest tensile strength. Conversely, a 50% fill ratio significantly reduced production costs, but at the expense of mechanical performance. Thus, if strength is the primary requirement, a higher fill ratio is recommended. The effect of printing speed was found to be less significant compared to layer height and filler ratio. The maximum recorded tensile strength was 540.65 N, achieved with a layer height of 0.5 mm, a 100% fill ratio, and a printing speed of 8 mm/s. In contrast, the lowest recorded tensile strength was 389.93 N, observed with a layer height of 0.4 mm, a 50% fill ratio, and a printing speed of 4 mm/s. After applying a transformation function, the data showed good alignment with the normal distribution on the probability plot, indicating that the assumption of normality was satisfied. Additionally, the incorporation of glass fibers significantly enhanced the mechanical strength of the printed samples.
A Semi-Analytical Method to Design a Dynamic Vibration Absorber for Coupled Plate Structures of Offshore Platforms
Authors: Du Yuan , Jia Fuxin , Tang Yang , Zheng Jiajun , Zou Yucheng , Ma Yong
Published: February 2025

Coupled plate structures composed of stiffened plates and sub-plates have been widely used in marine engineering practice. Meanwhile, the low-frequency multi-linear spectrum vibration control of the coupled stiffened plate structures has become necessary and meaningful. However, the design efficiency of the dynamic vibration absorber of the corresponding structure is still low. In the present study, a mathematical model of coupled plate structures and a dynamic vibration absorber is introduced to improve design efficiency. Subsequently, an experiment is designed to verify the effectiveness and advantages of the current method. The reliability of the current mathematical model is verified by comparing it with modal experiment results. Moreover, the equivalent mass solution efficiency is greatly improved by comparing it with FEM. Finally, a comparison experiment of the dynamic vibration absorber has also been conducted to further verify the effectiveness of the current method. The semi-analytical method proposed in the current research may be useful when designing dynamic vibration absorbers for the coupled plate structures of offshore platforms.
Unmasking AI’s Role in the Age of Disinformation: Friend or Foe?
Authors: García-Faroldi Livia , Teruel Laura , Blanco Sonia
Published: February 2025

This study addresses public perception of the relationship between artificial intelligence (AI) and disinformation. The level of general awareness of AI is considered, and based on this, an analysis is carried out of whether it may favor the creation and distribution of false content or, conversely, the public perceive its potential to counteract information disorders. A survey has been conducted on a representative sample of the Andalusian population aged 15 and over (1550 people). The results show that over 90% of the population have heard of AI, although it is less well known among the eldest age group (78%). There is a consensus that AI helps to produce (86%) and distribute (84%) fake news. Descriptive analyses show no major differences by sex, age, social class, ideology, type of activity or size of municipality, although those less educated tend to mention these negative effects to a lesser extent. However, 54% of the population consider that it may help in combating hoaxes, with women, the lower class and the left wing having positive views. Logistic regressions broadly confirm these results, showing that education, ideology and social class are the most relevant factors when explaining opinions about the role of AI in disinformation.
The Podcast Revolution? Exploring Journalistic Pioneers Beyond Legacy Media
Authors: Katzenberger Vera , Keil Jana , Wild Michael
Published: February 2025

Podcasts have established themselves in the digital media landscape as an integral part of information gathering and opinion formation for many users. The number of podcast users has stabilized at a high level in recent years. However, podcast producers, including podcast journalists, remain a largely unexplored group. This study focuses on podcast journalists and aims to identify the perceptions, motivations, and quality standards relating to their roles in podcasting. It is based on the results of an online survey of 378 podcast journalists from Germany, Austria, and Switzerland. Against a background of the concept of pioneer journalism, this article argues that podcast journalists are innovative contributors to the journalism ecosystem and have positioned themselves as new actors within the field. The findings of this study show that podcast journalists create, produce, and present journalistic content, for instance news or background stories, in the form of audio episodes, and see themselves as both educators and entertainers. They use the creative freedom of podcasting to engage deeply with their audiences and achieve high levels of listener loyalty. While financial gain is not their primary motivation, they have innovated new revenue models. They are committed to the quality of their content and emphasize comprehensibility and accuracy of information.
Uterine Microbiota and Bisphenols: Novel Influencers in Reproductive Health
Authors: Castellanos-Ruiz Dafne , Ojeda-Borbolla J. Gerardo , Ruiz-García Olga V. , Peña-Corona Sheila I. , Martínez-Peña Annia A. , Ibarra-Rubio María Elena , Gavilanes-Ruiz Marina , Mendoza-Rodríguez C. Adriana
Published: February 2025

Infertility affects 8–12% of couples worldwide, and 30–75% of preclinical pregnancy losses are due to a failure during the implantation process. Exposure to endocrine disruptors, like bisphenols, among others, has been associated with the increase in infertility observed in the past decades. An increase in infertility has correlated with exposure to endocrine disruptors like bisphenols. The uterus harbors its own microbiota, and changes in this microbiota have been linked to several gynecological conditions, including reproductive failure. There are no studies on the effects of bisphenols on the uterine–microbiota composition, but some inferences can be gleaned by looking at the gut. Bisphenols can alter the gut microbiota, and the molecular mechanism by which gut microbiota regulates intestinal permeability involves Toll-like receptors (TLRs) and tight junction (TJ) proteins. TJs participate in embryo implantation in the uterus, but bisphenol exposure disrupts the expression and localization of TJ proteins. The aim of this review is to summarize the current knowledge on the microbiota of the female reproductive tract (FRT), its association with different reproductive diseases—particularly reproductive failure—the effects of bisphenols on microbiota composition and reproductive health, and the molecular mechanisms regulating uterine–microbiota interactions crucial for embryo implantation. This review also highlights existing knowledge gaps and outlines research needs for future risk assessments regarding the effects of bisphenols on reproduction.
Preventative Vaccination of Nonhuman Primates
Authors: Nederlof Remco A. , Sainmaa Sanna , Wissink-Argilaga Nathalie , Koo Bon-Sang , Bakker Jaco
Published: February 2025

Vaccination constitutes one of the most important means of preventing infectious disease in captive nonhuman primates (NHPs). Vaccination protocols for NHPs vary, as they are mostly guided by institutional preference, infection pressure, local availability, and recommendations by non-peer reviewed resources. Currently, no updated literature review about vaccination options for NHP is available. Therefore, we provide a detailed overview of published vaccination options for NHP. Our findings demonstrate that, while there are often insufficient scientific data to justify their use, the core vaccines used in most NHP species confer protection against tetanus, rabies, and measles. Where information is available, efficacy expectations, adverse effects, dosages and frequency of administration are provided. We advocate that the decision to vaccinate NHP for less common diseases, for which an off-label vaccine is available, should be grounded in a comprehensive risk assessment. This assessment should consider factors specific to the individual animal, the vaccine, the housing institution, the epidemiology of the disease, and relevant regulatory and ethical considerations.
Influence of Urban Flooding on the Spatial Equity of Access to Emergency Medical Services Among Nursing Homes in Shanghai
Authors: Zhou Xueqing , Wang Shanshan , Yao Shenjun , Fang Lei
Published: February 2025

With the rapid aging of the population and increasing demand for elderly care services, ensuring equitable access to emergency medical service (EMS) for nursing homes has become a critical public health challenge. As the first Chinese city to experience an aging society, Shanghai faces compounding pressures from rapid urbanization and recurrent urban flooding, both of which exacerbate disparities in healthcare accessibility. This study investigates the spatial equity of EMS access among nursing homes in Shanghai, with a particular focus on the impacts of urban flooding. Using ordinary least squares and geographically weighted regression models, the study reveals that EMS accessibility is relatively equitable under normal conditions but deteriorates significantly during flood events, particularly in suburban and low-lying areas. The findings show that flood-induced disruptions to road networks disproportionately impact nursing homes in peripheral districts, widening accessibility gaps. Additionally, the study identifies that factors such as road density, emergency center distribution, and flood inundation depth play critical roles in shaping spatial equity. The results underscore the need for strategic interventions to enhance healthcare resilience, including optimized facility allocation and flood-resistant infrastructure. Policymakers should adopt integrated planning approaches to ensure equitable EMS access for vulnerable elderly populations during emergencies.
Political Parties as “Great Schools” of Civic Education
Authors: Postell Joseph
Published: February 2025

Current attempts to improve civic education through higher education should be supplemented by a focus on political parties, which have traditionally served as the “great schools” of civic education. America’s nineteenth-century parties drew voters out of their private concerns, engaged them in social life, and taught them to tolerate and bargain with each other. Legal changes over the past century have deprived them of the tools needed to fulfill this role. Policymakers should reconsider campaign finance laws that cripple parties, especially state and local organizations. Moreover, parties themselves should dedicate more time and resources to building a permanent presence in local communities and engaging citizens on the ground.
Evaluating Midazolam’s Influence on Bispectral Index and Propofol Concentrations Using Schnider and Eleveld Models in Target-Controlled Infusion General Anesthesia: A Prospective Observational Study
Authors: Linassi Federico , Zanatta Paolo , Kreuzer Matthias , Ciavattini Emma , Rizzetto Christian , Carron Michele
Published: February 2025

Background: Midazolam is widely used in clinical anesthesia, but its effects on the Bispectral Index (BIS) and propofol concentration at the effector site (CeP) are underexplored. This study investigates the pharmacodynamic interaction between midazolam and propofol in total intravenous anesthesia (TIVA) with target-controlled infusion (TCI), focusing on Schnider and Eleveld models. Methods: This prospective study included breast surgery patients receiving TIVA-TCI. BIS and CeP were assessed at loss of responsiveness (LoR), during maintenance (MA), and at return of responsiveness (RoR). Incidences of unwanted spontaneous responsiveness (USRE), burst suppression episodes (BSuppE), and postoperative delirium (POD) were recorded. Results: Midazolam premedication significantly reduced propofol doses and CeP at LoR and during MA, without affecting CeP at RoR. In the Schnider model, midazolam reduced total propofol dose, while in the Eleveld model, it lowered BIS at LoR. Unwanted anesthesia events occurred in 36.2% of patients, including USRE (10%), BSuppE (26.2%), and POD (1.2%). BSuppE rates were lower in the Schnider model and reduced in the midazolam group in the Eleveld model. Conclusions: Midazolam premedication influences CeP and BIS in TIVA-TCI, with model-specific variations, optimizing propofol management and improving patient outcomes.
Layer-by-Layer Nanoarchitectonics: A Method for Everything in Layered Structures
Authors: Ariga Katsuhiko
Published: February 2025

The development of functional materials and the use of nanotechnology are ongoing projects. These fields are closely linked, but there is a need to combine them more actively. Nanoarchitectonics, a concept that comes after nanotechnology, is ready to do this. Among the related research efforts, research into creating functional materials through the formation of thin layers on surfaces, molecular membranes, and multilayer structures of these materials have a lot of implications. Layered structures are especially important as a key part of nanoarchitectonics. The diversity of the components and materials used in layer-by-layer (LbL) assemblies is a notable feature. Examples of LbL assemblies introduced in this review article include quantum dots, nanoparticles, nanocrystals, nanowires, nanotubes, g-C3N4, graphene oxide, MXene, nanosheets, zeolites, nanoporous materials, sol–gel materials, layered double hydroxides, metal–organic frameworks, covalent organic frameworks, conducting polymers, dyes, DNAs, polysaccharides, nanocelluloses, peptides, proteins, lipid bilayers, photosystems, viruses, living cells, and tissues. These examples of LbL assembly show how useful and versatile it is. Finally, this review will consider future challenges in layer-by-layer nanoarchitectonics.
An Adaptive Learning Time Series Forecasting Model Based on Decoder Framework
Authors: Hao Jianlong , Sun Qiwei
Published: February 2025

Time series forecasting constitutes a fundamental technique for analyzing dynamic alterations within temporal datasets and predicting future trends in various domains. Nevertheless, achieving effective modeling faces challenges arising from complex factors such as accurately capturing the relationships among temporally distant data points and accommodating rapid shifts in data distributions over time. While Transformer-based models have demonstrated remarkable capabilities in handling long-range dependencies recently, directly applying them to address the evolving distributions within temporal datasets remains a challenging task. To tackle these issues, this paper presents an innovative sequence-to-sequence adaptive learning approach centered on decoder framework for addressing temporal modeling tasks. An end-to-end deep learning architecture-based Transformer decoding framework is introduced, which is capable of adaptively discerning the interdependencies within temporal datasets. Experiments carried out on multiple datasets indicate that the time series adaptive learning model based on the decoder achieved an overall reduction of 2.6% in MSE (Mean Squared Error) loss and 1.8% in MAE (Mean Absolute Error) loss when compared with the most advanced Transformer-based time series forecasting model.
Memetic-Based Biogeography Optimization Model for the Optimal Design of Mechanical Systems
Authors: Peixoto Arcílio Carlos Ferreira , António Carlos A. Conceição
Published: February 2025

The science of biogeography was described through mathematical equations in 1967 by Robert MacArthur and Edward Wilson. In 2008, Dan Simon presented an algorithm called biogeography-based optimization, or BBO, which used some of the principles and definitions described in MacArthur and Wilson’s book. The objectives of this work were to study the behavior of the BBO method when it is hybridized with other evolutionary search methods and to analyze the effect of its application to some examples of mechanical engineering systems. The operators considered in the hybridization study are genetic recombination (crossover) and local search, aiming to overcome the limitations and difficulties that arise when using the original BBO. The results of the original BBO were promising in the context of a global search. However, there is a diversity problem that does not allow for good quality increments in the final phase of the evolutionary process. The additional modifications included, such as the concept of blending in migration, the cycle of mutations and the replacement of the worst solutions by injection of new ones, all show positive effects on the method’s performance. However, the biggest increase happened with the implementation of the hybridization processes. Crossover improved the speed and diversity of the population in some cases, while local search helped the algorithm in later generations, allowing it to quickly reach the optimum point. With this mentioned, it is important to note that the best results were all obtained with the fully modified algorithm. Statistical tests were implemented to validate the significance of changes due to modifications included in the original proposal of BBO.
On the Oscillation of Fourth-Order Delay Differential Equations via Riccati Transformation
Authors: Mazen Mohamed , El-Sheikh Mohamed M. A. , Euat Tallah Samah , Ismail Gamal A. F.
Published: February 2025

This paper deals with the oscillatory behavior of solutions of a general class of fourth-order non-linear delay differential equations. New oscillation criteria are established using Riccati transformation and a Philos-type technique. The obtained results not only improve and extend some published results in the literature, but also relax some traditional conditions on the function ψ(χ(ι)). Three examples are provided to illustrate the main results.
Static-Aperture Synthesis Method in Remote Sensing and Non-Destructive Testing Applications
Authors: Inkarbaieva Olha , Kolesnikov Denys , Kovalchuk Danyil , Pavlikov Volodymyr , Ponomaryov Volodymyr , Garcia-Salgado Beatriz , Volosyuk Valerii , Zhyla Semen
Published: February 2025

The study is dedicated to the statistical optimization of radar imaging of surfaces with the synthetic aperture radar (SAR) technique, assuming a static surface area and applying the ability to move a sensor along a nonlinear trajectory via developing a new method and validating its operability for remote sensing and non-destructive testing. The developed models address the sensing geometry for signals reflected from a surface along with the observation signal–noise equation, including correlation properties. Moreover, the optimal procedures for coherent radar imaging of surfaces with the static SAR technology are synthesized according to the maximum likelihood estimation (MLE). The features of the synthesized algorithm are the decoherence of the received oscillations, the matched filtering of the received signals, and the possibility of using continuous signal coherence. Furthermore, the developed optimal and quasi-optimal algorithms derived from the proposed MLE have been investigated. The novel framework for radio imaging has demonstrated good overall operability and efficiency during simulation modeling (using the MATLAB environment) for real sensing scenes. The developed algorithms of spatio–temporal signal processing in systems with a synthesized antenna with nonlinear carrier trajectories open a promising direction for creating new methods of high-precision radio imaging from UAVs and helicopters.
Time-Synchronized Fault-Tolerant Control for Robotic Manipulators
Authors: Wang Duansong , Zhang Gang , Chen Rui , Zhang Jinzhong , Zhang Tan
Published: February 2025

This article proposes a time-synchronized fault-tolerant convergence control method for n-degree-of-freedom robotic manipulators. The main challenge lies in driving the tracking errors of all joints to converge simultaneously, especially in the presence of system faults, external disturbances, and model uncertainties. We introduce a normalized sign function that guarantees the property of ratio persistence for all joints and plays a crucial role in time-synchronized convergence control. A time-synchronized convergence observer is proposed that not only adopts a time-synchronized convergence control framework but also overcomes the lumped uncertainty term, which includes the system fault components, external disturbances, and system uncertainties. A salient feature of this method is that, regardless of the initial state and various uncertainties, each component of the robot manipulator system can simultaneously converge to an equilibrium point. Simulations conducted on a two-link robotic manipulator demonstrate the notable benefits of the designed time-synchronized control method, as evidenced by the comparative results.
Structural Characterization and Anticoagulant Potential of Colochirus quadrangularis Fucosylated Glycosaminoglycan 5−12 Oligomers with Unusual Branches
Authors: Zhang Xuedong , Yan Guangwei , Liu Xinming , Fu Jiewen , Shi Xiang , Cao Pei , Sun Yuqian , Zhong Shengping , Nong Jiale , Jiang Peiqi , Liu Yonghong , Zhang Baoshun , Yuan Qingxia , Zhao Longyan
Published: February 2025

The depolymerized products and oligosaccharide fractions from sea cucumber fucosylated glycosaminoglycans (FGs) are promising anticoagulant candidates, and more novel FG-derived oligosaccharides from low-priced sea cucumbers are expected to be obtained. This study isolated 5−12 oligomers (OF1−OF3) with unusual branches from β-eliminative depolymerized products of Colochirus quadrangularis FG (CqFG). Detailed NMR analyses showed that OF1−OF3 consisted of a chondroitin 4,6-sulfates backbone and some sulfated fucosyl branches (FucS), including monosaccharides (α-l-Fuc2S4S, α-l-Fuc3S, α-l-Fuc4S, α-l-Fuc2S3S4S, and α-l-Fuc2S) and a disaccharide D-Gal3S4S-α1,3-l-Fuc2S4S with the ratio of ~36:35:10:7:3:9, attached to the C-3 position of β-d-GlcA or its derivatives, such as α-l-Δ4,5GlcA and β-d-GlcA-ol. Unusually, α-l-Fuc3S was the main FucS branch; no α-l-Fuc3S4S branch was found, and α-l-Fuc2S3S4S and α-l-Fuc2S branches were also found in OF1–OF3. The OF2 and OF3 could strongly inhibit the intrinsic and common coagulation pathways. Intrinsic FXase is a target of OF2 and OF3 inhibiting the intrinsic coagulation pathways, and the unusual side chains may increase the intrinsic FXase inhibitory activity. OF2 and OF3 showed negligible bleeding risk, and less bleeding than heparin (HP), low-molecular-weight heparins (LMWHs), and CqFG. These findings support novel FG oligosaccharides with some unusual branches from low-priced sea cucumbers to be prepared as safer anticoagulants.
Mechanism of OH*-Modified 4H-SiC Surface with Scratches Based on ReaxFF MD Simulation
Authors: Yan Dongxiao , Huang Hui , Xue Mingpu , Duan Nian
Published: February 2025

OH* generated through plasma catalysis offers several advantages, including a long survival time, high modification efficiency, and environmental friendliness. Consequently, a plasma-assisted polishing technology has rapidly developed. Previous studies exploring the interaction mechanism between OH* and 4H-SiC have often assumed flat surfaces. However, in the surface modification experiments on 4H-SiC, the actual surface morphology was not flat but contained numerous scratches. Therefore, this study investigated the interaction mechanism of OH* on an uneven surface using reactive force field molecular dynamics (ReaxFF MD) simulations. The results show that in the low-speed OH* modification process, the adsorption effect leads to a thicker modified layer at higher locations than at lower locations. The resulting modified layer can be removed by soft abrasive mechanical polishing to achieve surface flatness, but there will be a modified layer on the surface, which needs to be modified and polished several times. In contrast, during high-speed OH* modification, high-speed particle bombardment causes more Si-O bonds to diffuse into the scratches, resulting in the formation of a flat bonding layer with surface planarization achieved after a single polishing step. The interaction mechanism of OH* with the uneven surface at different speeds, as obtained through ReaxFF MD, provides a theoretical foundation for subsequent polishing experiments.
QuantiFERON® Monitor Test as a Potential Tool for Stratifying Patients by Infection Risk and Tailoring Follow-Up Care in Lung Transplant Recipients: A Single-Center Retrospective Experience
Authors: Solidoro Paolo , Curtoni Antonio , Patrucco Filippo , Russo Eleonora , Sidoti Francesca , Piccinini Giorgia , Bondi Alessandro , Valesella Paolo , Genco Mattia , Boffini Massimo , Rinaldo Rocco Francesco , Costa Cristina
Published: February 2025

Background: Lung transplantation is a life-saving option for patients with end-stage respiratory diseases, but risk of infections remains critical for ensuring long-term organ function. This study aimed to assess immune recovery in lung transplant recipients by measuring IFN-γ levels using the QuantiFERON Monitor Test (QFM). Results were correlated with episodes of infection and organ rejection to explore the assay’s predictive potential. Methods: A retrospective study was conducted on 15 lung transplant recipients at the Lung Transplant Centre of Turin (Città della Salute e della Scienza di Torino, Italy) between December 2019 and January 2023. Patients were divided into a High Infection (HI) group (with >3 infections) and Low Infection (LI) group (with ≤3 infections). QFM assays were performed after 18 months post-transplant. Results: HI patients had lower QFM levels compared to LI (68.84 ± 21.98 vs. 380.54 ± 104.64 UI/mL, p = 0.033). A QFM value <89.5 UI/mL was associated with increased infection risk (p < 0.05). Patients with lower QFM levels also exhibited higher rates of MRSA bacteremia during hospitalization (50% HI vs. 0% LI, p = 0.04). No differences were observed in acute or chronic rejection rates, but LI patients showed more frequent alveolar neutrophilia at the fourth month post-transplant (0% HI vs. 55.5% LI, p = 0.04). Conclusion: lower QFM values were associated with higher infection risk, highlighting the assay’s potential for immune monitoring. In this study, a QFM value of 89.5 UI/mL showed good predictive accuracy for infections beyond 18 months. Further studies are needed to refine QFM’s role in post-transplant care.
Synthesis of (1,10-Phenanthroline-κ2N,N′)(β2-Methyl- and β2-PhenylAlaninate-κ2N,O)Copper(II) Nitrate Complexes and Their Antiproliferative Activity on MCF-7 and A549 Cancer Cell Lines
Authors: Chavelas-Hernández Leticia , Hernández-Vázquez Luis G. , Valdez-Camacho Jonathan R. , Espinoza-Guillén Adrián , Tavira-Montalvan Carlos A. , Meneses-Acosta Angélica , Juaristi Eusebio , Ruiz-Azuara Lena , Escalante Jaime
Published: February 2025

In recent years, metallodrugs have been playing an important role, showing to be more efficient in the treatment of several diseases, such as cancer. Indeed, it is important to synthesize novel molecules to be used as more effective agents against cancer. In the present paper, the synthesis of two new molecules belonging to Casiopeínas® is reported. These compounds present a β2-aminoacidate derivative as the secondary ligand. The novel metal complexes were characterized by high-resolution mass spectrometry, FT-IR, UV-Vis, EPR, effective magnetic moment and cyclic voltammetry measurements, and single crystal X-ray diffraction analysis. Furthermore, these compounds were evaluated in vitro against the cancer lines MCF-7 (breast cancer) and A549 (lung cancer).
In Silico Characterization of Sirtuins in Acetic Acid Bacteria Reveals a Novel Phylogenetically Distinctive Group
Authors: Jugović Igor , Trček Janja
Published: February 2025

Acetic acid bacteria are single-celled organisms well-known for their ability to convert ethanol into acetic acid. Still, recent research suggests they may harbor another attractive characteristic—the production of proteins with remarkable similarities to sirtuins. Sirtuins have been linked to lifespan extension in various organisms, raising intriguing questions about the potential connection between acetic acid bacteria and the biology of aging. This article delves into the characterization of sirtuin homologs in acetic acid bacteria. Up to three types of sirtuin homologs have been identified in 21% of acetic acid bacteria genomes deposited in NCBI. All three types were present only in the genera Acetobacter and Novacetimonas, which are known to survive in the harshest environmental conditions (high concentrations of acetic acid and ethanol). Interestingly, two types of these sirtuin homologs (SirAAB-L and SirAAB-S) constitute a separate group (SirAAB), distinctive from all other presently known sirtuins. The results obtained in silico thus encourage further studies into the function of these types of sirtuins and their interplay with metabolic pathways in these industrially important bacteria.
Potential of the Nano-Encapsulation of Antioxidant Molecules in Wound Healing Applications: An Innovative Strategy to Enhance the Bio-Profile
Authors: Hallan Supandeep Singh , Ferrara Francesca , Cortesi Rita , Sguizzato Maddalena
Published: February 2025

Naturally available antioxidants offer remarkable medicinal applications in wound healing. However, the encapsulation of these phytoactive moieties into suitable nano-scale drug delivery systems has always been challenging due to their inherent characteristics, such as low molecular weight, poor aqueous solubility, and inadequate skin permeability. Here, we provide a systematic review focusing on the major obstacles hindering the development of various lipid and polymer-based drug transporters to carry these cargos to the targeted site. Additionally, this review covers the possibility of combining the effects of a polymer and a lipid within one system, which could increase the skin permeability threshold. Moreover, the lack of suitable physical characterization techniques and the challenges associated with scaling up the progression of these nano-carriers limit their utility in biomedical applications. In this context, consistent progressive approaches for addressing these shortcomings are introduced, and their prospects are discussed in detail.
Research Advances and Future Perspectives of Superhydrophobic Coatings in Sports Equipment Applications
Authors: Huang Guoyuan , Guo Yingqing , Lee Byungchan , Chen Hong , Mao Aqiang
Published: February 2025

In recent years, superhydrophobic coatings have attracted much attention due to their excellent water repellency and self-cleaning properties. They have broad application prospects in improving the performance and durability of sports equipment (such as clothing, footwear, balls, and protective equipment). Recent studies have shown that these coatings can improve water repellency, reduce friction, enhance traction, and extend the service life of sports equipment by preventing water absorption and reducing dirt accumulation. Despite their potential, the practical application of superhydrophobic coatings still faces many challenges, including difficulties in coating preparation, limited long-term durability, and high production costs that prevent large-scale production. This paper begins with an analysis of the current status of superhydrophobic coatings in sports equipment, from theory to application, from the basic Young’s model to the novel Contact Line Pinning Model, analyzing the advantages and disadvantages of several methods in some aspects, focusing on the most commonly used preparation methods, including the template method, the gel–gel method, the deposition method, etc., and objectively analyzing the preparation methods to match the appropriate sports equipment applications. Despite these advances, there are still significant gaps in understanding the long-term performance of these coatings under real-world conditions. The paper concludes by identifying future research directions, with a focus on improving wear resistance, improving cost-effectiveness, and assessing the environmental impact of these materials. These insights will contribute to the continued development and application of superhydrophobic coatings in the field of sports equipment.
A Comprehensive Analysis of Non-Thermal Ultrasonic-Assisted Extraction of Bioactive Compounds from Citrus Peel Waste Through a One-Factor-at-a-Time Approach
Authors: Xuereb Matthew A. , Psakis Georgios , Attard Karen , Lia Frederick , Gatt Ruben
Published: February 2025

Food waste presents a critical environmental and economic challenge across Europe. In the Mediterranean region, the agricultural industry generates considerable quantities of citrus fruits, leading to significant byproduct waste, which remains underutilized. To help address this, this study explored the valorization of orange peel waste using non-thermal ultrasonic-assisted extraction (UAE) and a one-factor-at-a-time experimental design to investigate the effects of nine chemical and physical UAE parameters. The goal was to identify ideal operational ranges for each parameter using several responses (bioactive compound recovery, antioxidant activity, and radical scavenging activity), thus elucidating the most influential UAE factors and their role in co-extracting various classes of natural compounds. The key findings revealed that the polarity and ionic potential of the extraction medium, tuned through ethanol:water or pH, significantly influenced both the chemical profile and bioactivity of the extracts. Notably, citric acid and citrates appeared to stabilize co-extracted compounds. Lower solid-to-liquid ratios increased yields, while particle sizes between 1400 and 710 µm enhanced phenolic recovery by approximately 150 mg/L GAE. In contrast, increases in pulse, probe diameter, immersion depth, and extraction time led to degradation of bioactive compounds, whereas the maximal amplitude improved phenolic acid recovery by up to 2-fold. Collectively, these insights provide a foundation for optimizing non-thermal UAE to valorize orange peel waste.
The Proteome Content of Blood Clots Observed Under Different Conditions: Successful Role in Predicting Clot Amyloid(ogenicity)
Authors: Kell Douglas B. , Pretorius Etheresia
Published: February 2025

A recent analysis compared the proteome of (i) blood clots seen in two diseases—sepsis and long COVID—when blood was known to have clotted into an amyloid microclot form (as judged by staining with the fluorogenic amyloid stain thioflavin T) with (ii) that of those non-amyloid clots considered to have formed normally. Such fibrinaloid microclots are also relatively resistant to fibrinolysis. The proteins that the amyloid microclots contained differed markedly both from the soluble proteome of typical plasma and that of normal clots, and also between the diseases studied (an acute syndrome in the form of sepsis in an ITU and a chronic disease represented by Long COVID). Many proteins in the amyloid microclots were low in concentration in plasma and were effectively accumulated into the fibres, whereas many other abundant plasma proteins were excluded. The proteins found in the microclots associated with the diseases also tended to be themselves amyloidogenic. We here ask effectively the inverse question. This is: can the clot proteome tell us whether the clots associated with a particular disease contained proteins that are observed uniquely (or are highly over-represented) in known amyloid clots relative to normal clots, and thus were in fact amyloid in nature? The answer is in the affirmative in a variety of major coagulopathies, viz., venous thromboembolism, pulmonary embolism, deep vein thrombosis, various cardiac issues, and ischaemic stroke. Galectin-3-binding protein and thrombospondin-1 seem to be especially widely associated with amyloid-type clots, and the latter has indeed been shown to be incorporated into growing fibrin fibres. These may consequently provide useful biomarkers with a mechanistic basis.
The Reconstructed Individual Patient Data from Kaplan–Meier (IPDfromKM) Method for Non-Inferiority Analyses: A New Potential Application
Authors: Piragine Eugenia , Trippoli Sabrina , Veneziano Sara , Messori Andrea , Calderone Vincenzo
Published: February 2025

The IPDfromKM method, or Shiny method, is an artificial intelligence tool that enables indirect comparisons between studies by reconstructing individual patient data (IPD) from Kaplan–Meier (KM) curves. The IPDfromKM method is generally used for superiority analyses, but a further application could be represented by non-inferiority analyses. However, there are no studies supporting this methodological hypothesis. The aim of this work was to validate the IPDfromKM method for non-inferiority analyses by “exploiting” the well-described non-inferiority of implantable devices occluding the left atrial appendage compared to oral anticoagulants in patients with atrial fibrillation. We performed a systematic review searching for randomized controlled trials (RCTs) in the PubMed database and found five studies. The R software (version 4.3.3) was used to perform a standard survival analysis comparing Watchman and Amlet devices with warfarin. The hazard ratio (HR), with 95% confidence interval (CI), was the main parameter of our analysis. The results confirmed the non-inferiority of Amlet and Watchman compared to warfarin (HR of Watchman vs. warfarin: 1.23, 95% CI 0.80 to 1.9; HR of Amlet vs. warfarin: 1.05, 95% CI 0.61 to 1.80). Therefore, we proposed a new application of the IPDfromKM method that could be potentially relevant in decision-making for the management of this common cardiac arrhythmia and a wide range of other pathological conditions.
Imaging Flow Cytometry in HIV Infection Research: Advantages and Opportunities
Authors: Elfimov Kirill. A. , Baboshko Dmitriy. A. , Gashnikova Natalya. M.
Published: February 2025

The human immunodeficiency virus (HIV) is a type of retrovirus that infects humans and belongs to the Lentivirus group. Despite the availability of effective treatments, HIV infections are still increasing in some parts of the world, according to the World Health Organization (WHO). Another major challenge is the growing problem of HIV becoming resistant to drugs. This highlights the importance of ongoing research to better understand HIV and find new ways to stop the virus from spreading in the body. Scientists use a variety of methods to study HIV, including techniques from molecular and cellular biology. Many of these methods rely on fluorescent dyes to help visualize specific parts of the virus or infected cells. This article focuses on a technique called imaging flow cytometry, which is particularly useful for studying HIV. Imaging flow cytometry is unique because it not only measures fluorescence (light emitted by the dyes) but also captures images of each cell being analyzed. This allows researchers to see where the fluorescence is located within the cell and to study the cell’s shape and structure in detail. Additionally, this method can be combined with machine learning to analyze large amounts of data more efficiently.
EGCG Alleviates DSS-Induced Colitis by Inhibiting Ferroptosis Through the Activation of the Nrf2-GPX4 Pathway and Enhancing Iron Metabolism
Authors: Chen Junzhou , Yin Conghui , Zhang Yilong , Lai Xin , Liu Chen , Luo Yuheng , Luo Junqiu , He Jun , Yu Bing , Wang Quyuan , Wang Huifen , Chen Daiwen , Wu Aimin
Published: February 2025

Background: Ferroptosis is a regulated cell death process linked to various diseases. This study explored whether Epigallocatechin-3-gallate (EGCG), a tea-derived antioxidant, could regulate ferroptosis to alleviate dextran sulfate sodium (DSS)-induced colitis. Methods: A DSS-induced colitis model was used to assess EGCG’s effects. Ferroptosis markers, oxidative stress, and iron metabolism were evaluated, alongside Nrf2-GPX4 pathway activation and ferritin (FTH/L) expression. Results: Iron dysregulation and oxidative stress contributed to DSS-induced colitis by activating ferroptosis in colonic epithelial cells. EGCG supplementation inhibited ferroptosis, reducing oxidative damage. Mechanistically, EGCG activated the Nrf2-GPX4 pathway, enhancing antioxidant defense, and improved iron metabolism by upregulating ferritin expression. Conclusions: EGCG effectively suppressed DSS-induced ferroptosis and colitis, highlighting its potential as a ferroptosis inhibitor and therapeutic agent.