bluuebunny/crossref_metadata_2025 huggingface dataset:
mBeRFP, a versatile fluorescent tool to enhance multichannel live imaging and its applications
Authors: Martin Emmanuel , Suzanne Magali
Published: January 2022

Cell and developmental biology increasingly require live imaging of protein dynamics in cells, tissues or living organisms. Thanks to the discovery and the development of a panel of fluorescent proteins over the last decades, live imaging has become a powerful and commonly used approach. However, multicolor live imaging remains challenging. The generation of long Stokes shift red fluorescent proteins, such as mBeRFP, offers interesting new perspectives to bypass this limitation. Here, we constructed a set of mBeRFP-expressing vectors and provided a detailed characterization of this fluorescent protein for in vivo live imaging and its applications in Drosophila. Briefly, we showed that a single illumination source is sufficient to simultaneously stimulate mBeRFP and GFP. We demonstrated that mBeRFP can be easily combined with classical green and red fluorescent protein without any crosstalk. We also showed that the low photobleaching of mBeRFP is suitable for live imaging, and that this protein can be used for quantitative applications such as FRAP or laser ablation. Finally, we believe that this fluorescent protein, with the set of new possibilities it offers, constitutes an important tool for cell, developmental and mechano biologists in their current research.
Clustering Deviation Index (CDI): A robust and accurate unsupervised measure for evaluating scRNA-seq data clustering
Authors: Fang Jiyuan , Chan Cliburn , Owzar Kouros , Wang Liuyang , Qin Diyuan , Li Qi-Jing , Xie Jichun
Published: January 2022

Single-cell RNA-sequencing (scRNA-seq) technology allows us to explore cellular heterogeneity in the transcriptome. Because most scRNA-seq data analyses begin with cell clustering, its accuracy considerably impacts the validity of downstream analyses. Although many clustering methods have been developed, few tools are available to evaluate the clustering "goodness-of-fit" to the scRNA-seq data. In this paper, we propose a new Clustering Deviation Index (CDI) that measures the deviation of any clustering label set from the observed single-cell data. We conduct in silico and experimental scRNA-seq studies to show that CDI can select the optimal clustering label set. Particularly, CDI also informs the optimal tuning parameters for any given clustering method and the correct number of cluster components.
The RNA binding protein Swm is critical for Drosophila melanogaster intestinal progenitor cell maintenance
Authors: Ariyapala Ishara S. , Buddika Kasun , Hundley Heather A. , Calvi Brian R. , Sokol Nicholas S.
Published: January 2022

The regulation of stem cell survival, self-renewal, and differentiation is critical for the maintenance of tissue homeostasis. Although the involvement of signaling pathways and transcriptional control mechanisms in stem cell regulation have been extensively investigated, the role of post-transcriptional control is still poorly understood. Here we show that the nuclear activity of the RNA-binding protein Second Mitotic Wave Missing (Swm) is critical for Drosophila intestinal stem cells (ISCs) and their daughter cells, enteroblasts (EBs), to maintain their identity and function. Loss of swm in these intestinal progenitor cells leads ISCs and EBs to lose defined cell identities, fail to proliferate, and detach from the basement membrane, resulting in severe progenitor cell loss. swm loss further causes nuclear accumulation of poly(A)+ RNA in progenitor cells. Swm associates with transcripts involved in epithelial cell maintenance and adhesion, and the loss of swm, while not generally affecting the levels of these Swm-bound mRNAs, leads to elevated expression of proteins encoded by some of them, including the fly orthologs of Filamin and Talin. Taken together, this study indicates a role for Swm in adult stem cell maintenance, and raises the possibility that nuclear post-transcriptional gene regulation plays vital roles in controlling adult stem cell maintenance and function.
E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins
Authors: Huang Shuai A. , Chin Hui San , Reljic Boris , Djajawi Tirta M. , Tan Iris K.L. , Stroud David A. , Huang David C.S. , van Delft Mark F. , Dewson Grant
Published: January 2022

Intrinsic apoptosis is principally governed by the BCL-2 family of proteins, but some non-BCL-2 proteins are also critical to control this process. To identify novel apoptosis regulators, we performed a genome-wide CRISPR-Cas9 library screen, and it identified the mitochondrial E3 ubiquitin ligase MARCHF5/MITOL/RNF153 as an important regulator of BAK apoptotic function. Deleting MARCHF5 in diverse cell lines dependent on BAK conferred profound resistance to BH3-mimetic drugs. The loss of MARCHF5 or its E3 ubiquitin ligase activity surprisingly drove BAK to adopt an activated conformation, with resistance to BH3-mimetics afforded by the formation of inhibitory complexes with pro-survival proteins MCL-1 and BCL-XL. Importantly, these changes to BAK conformation and pro-survival association occurred independently of BH3-only proteins and influence on pro-survival proteins. This study identifies a new mechanism by which MARCHF5 regulates apoptotic cell death and provides new insight into how cancer cells respond to BH3-mimetic drugs. These data also highlight the emerging role of ubiquitin signalling in apoptosis that may be exploited therapeutically.
S100A4 exerts robust mucosal adjuvant activity for co-administered antigens in mice
Authors: Sen Chaudhuri Arka , Yeh Yu-Wen , Sun Jia-Bin , Zewdie Olifan , Jin Tao , Wei Bin , Holmgren Jan , Zou Xiang
Published: January 2022

The lack of clinically applicable mucosal adjuvants is a major hurdle in designing effective mucosal vaccines. We hereby report that the calcium-binding protein S100A4, which regulates a wide range of biological functions, is a potent mucosal adjuvant in mice for co-administered antigens, including the SARS-CoV-2 spike protein, with comparable or even superior efficacy as cholera toxin but without causing any adverse reactions. Intranasal immunization with recombinant S100A4 elicited antigen-specific antibody and pulmonary cytotoxic T cell responses, and these responses were remarkably sustained for longer than six months. As a self-protein, S100A4 did not stimulate antibody responses against itself, a quality desired of adjuvants. S100A4 prolonged nasal residence of intranasally delivered antigens and promoted migration of antigen-presenting cells. S100A4-pulsed dendritic cells potently activated cognate T cells. Furthermore, S100A4 induced strong germinal center responses revealed by both microscopy and mass spectrometry, a novel technique for measuring germinal center activity. In conclusion, S100A4 may be a promising adjuvant in formulating mucosal vaccines, including vaccines against pathogens that infect via the respiratory tract, such as SARS-CoV-2.
The generation of a Nutm1 knock-in reporter mouse line for imaging post-meiotic spermatogenesis
Authors: Hakun Maxwell Claef , Rossant Janet , Gu Bin
Published: January 2022

Spermiogenesis, the post-meiotic stage of sperm development, is critical for normal male fertility. Many genetic defects and environmental assaults that affect spermiogenesis have been shown to be associated with male infertility. In addition, this later stage of spermatogenesis has been proposed to be an ideal target for male contraceptive development. The mouse is a widely used model for studying the mechanisms of spermatogenesis and spermiogenesis. However, due to the complexity and the asynchronous nature of spermatogenesis in adult testis, it is challenging to study molecular processes restricted to this specific developmental stage. It is also challenging to monitor the spermiogenesic activity in live mice, which is critical for screening for fertility-modulating interventions such as contraceptives. Here we reported the development of a Nutm1-T2A- luciferase 2(Luc2)-tandem Tomato(TdTomato) knock-in reporter mouse model that specifically labels post-meiotic spermatids. Homozygous reporter mice are healthy and fully fertile, demonstrating no interference with the normal functions of the Nutm1 gene by the reporter. We demonstrated the visualization of post-meiotic spermatids by fluorescent imaging of the TdTomato reporter in both live and fixed testis tissues. We also demonstrated bioluminescence imaging of Nutm1 expressing cells in live mice. The Nutm1-T2A-Luc2TdTomato reporter mouse can serve as a valuable tool for studying spermiogenesis.
Positioning big data analytics capabilities towards financial service agility
Authors: Edu Abeeku Sam
Published: January 2022

Purpose

Enterprises are increasingly taking actionable steps to transform existing business models through digital technologies for service transformation such as big data analytics (BDA). BDA capabilities offer financial institutions to source financial data, analyse data, insight and store such data and information on collaborative platforms for a quick decision-making process. Accordingly, this study identifies how BDA capabilities can be deployed to provide significant improvement for financial services agility.

Design/methodology/approach

The study relied on survey data from 485 banking professionals' perspectives with BDA usage, IT capability development and financial service agility. The PLS-SEM technique was used to evaluate the underlying relationship and the applicability of the research framework proposed.

Findings

Based on the empirical test from this study, distinctive BDA usage grounded on the concept of IT capability viewpoint proof that financial service agility could be enhanced provided enterprises develop technical capabilities alongside other relevant resources.

Practical implications

The study further highlights the need for financial service managers to identify BDA technologies such as data mining, query and reporting, data visualisation, predictive modelling, streaming analytics, video analytics and voice analytics to focus on financial knowledge gathering and market observation. Financial managers can also deploy BDA tools to develop a strategic road map for data management, data transferability and knowledge discovery for customised financial products.

Originality/value

This study is a useful contribution to the burgeoning discussion with emerging technologies such as BDA implication to improving enterprises operations.
Digital technologies, sustainable open innovation and shared value creation: evidence from an Italian agritech business
Authors: Rialti Riccardo , Marrucci Anna , Zollo Lamberto , Ciappei Cristiano
Published: January 2022

Purpose

The aim of this research was to explore the mechanisms underpinning open innovation (OI) success and its sustainability in agrifood businesses. First, the authors explored the importance of 4.0 technologies in data collection from crowds, subsequently examining how new technologies might drive forward the development of collaborative strategies with suppliers. Reductions in resource wasting were observed. The role of 4.0 technologies in increasing supply chain sustainability overall was the main focus of the study.

Design/methodology/approach

This research builds on a single inductive case study method. The authors performed an in-depth analysis of data from an Italian agritech distributor. Data – aside from multiple semi-structured interviews – were collected via several different sources. The results have been summarized in an integrated holistic conceptual framework.

Findings

The findings show that 4.0 technologies allow for swift information exchanges between consumers, the agritech business and suppliers. As a result, consumers might demand new products and, consequently, the agritech business can arrange new offerings with suppliers, completing the OI and shared value creation circle. Likewise, the possibility of adopting a just-in-time approach of sorts may reduce the wasting of resources. The absorptive capacities and knowledge management capabilities of the agritech business play a fundamental role in OI performance, sustainability and success.

Originality/value

This research seminally explores how 4.0 technologies and knowledge management techniques can enable OI in agrifood businesses. Additionally, the ways in which OI may foster the development of sustainability-orientated supply chain strategies have been conceptualized.
Segmentation of wine tourism experience in Mexican wine regions using netnography
Authors: Hernández Ana Laura , Alarcón Silverio , Meraz Ruiz Lino
Published: January 2022

Purpose

This paper aims to offer an experiential segmentation based on the analysis of comments from wine tourists from different Mexican wine regions, using netnography as a data collection and analysis tool.

Design/methodology/approach

The netnography methodology was applied in three steps: first, collecting consumer opinions (TripAdvisor 1,240 opinions, 2017–2019) from their visits to 20 wineries in four Mexican wine regions. Second, organizing of the data by classifying the various experiences. Third, by the application of multiple correspondence and cluster analysis to consolidate four segments taking as reference the 4Es Model.

Findings

The results show that of the four Mexican wine regions, Baja California is divided between the aesthetic and wine focus segments, Coahuila is more associated to the educational segment, while tourist who visit Queretaro tend to relate to the activities of the entertainment segment.

Research limitations/implications

The scarce literature that exists regarding wine tourism in Mexico made certain comparisons and relationships to the results difficult to establish.

Practical implications

The results provide stakeholders (wineries, state tourism departments, wine tourism marketers and wine tourism researchers) a segmentation proposal focused on tourist experiences to improve marketing programs and wine tourism offerings.

Originality/value

The use of netnography as a tool for wine tourism research in Mexico is one that has not been previously explored. In addition, this study considers different Mexican wine regions, which allows for comparisons and relationships between them that can contribute to greater market differentiation.
Understanding fire learning experiences in Aotearoa New Zealand
Authors: Rhodes Amelia Jane , Tyler Nichola
Published: January 2022

Purpose

This paper aims to present exploratory research on how people in Aotearoa New Zealand experience and learn about fire, and how they think and feel about fire as adults.

Design/methodology/approach

A qualitative survey with a sample of 40 young adults aged 18–23 years in Aotearoa New Zealand were recruited through Prolific Academic. Reflexive thematic analysis was used to construct themes across participants experiences.

Findings

Four themes were constructed that described participants’ learning about fire and were named influence of context and internal responses to fire, development of normative beliefs about fire, learning how and when fire can be used and learning about fire safety. Two themes were developed that described participants thoughts and feelings about fire as an adult. These were named knowledge is power and emotional congruence with fire. Results highlight the significant role of parental modelling, reinforcement and sensory experiences in the way individuals experience and learn about fire.

Practical implications

Understanding fire learning is important for establishing which experiences may lead to appropriate and inappropriate fire use which in turn can inform fire prevention initiatives.

Originality/value

To the best of the authors’ knowledge, this research represents one of the first studies to directly examine fire learning in the general population.
Monkey see, monkey do? Examining the effect of entrepreneurial orientation and knowledge sharing on new venture creation for Gen Y and Gen Z
Authors: Hamdi Muhammad , Indarti Nurul , Manik Hardo Firmana Given Grace , Lukito-Budi Andy Susilo
Published: January 2022

Purpose

This study aims to examine the effect of entrepreneurial intention and attitude towards knowledge sharing on new business creation by comparing two generations, Y generation (millennials) and Z generation (post-millennial). In addition, the current study uses a social cognitive theory as a point of departure to test the research hypotheses.

Design/methodology/approach

This study deploys a quantitative approach (hypothetic-deductive approach) by surveying 300 respondents representing the two Indonesian generations. The questionnaire consisting of demographic items (age, education, etc.) and variables was the primary research instrument. This study used regression analysis, a Wald test for examining the proposed hypotheses and a t-test to provide a deeper analysis of the findings.

Findings

Findings from the current study show that Gen Y is still seeking a balance for their learning sources by involving in their social environments as well as exploring the digital world. In contrast, Gen Z is much more dominant in the independence to learn things that interest them. They have less dependency on social patrons but prioritise themselves as the leading model.

Practical implications

The findings of this study provide practical implications for higher education institutions in the development of entrepreneurship education to achieve learning effectiveness.

Originality/value

This study aims to contribute by providing empirical evidence in the effect of entrepreneurial orientation and attitude towards knowledge sharing on new venture creation with particular reference to Gen Y and Gen Z, suggested by previous studies. Although Gen Y and Gen Z are digital natives, this study provides insight into a shift in the characteristic of two generations, as also found in comparison to previous generations, such as Baby-Boomer vs Gen X and Gen X vs Gen Y. This study proclaims the need to adjust organisational theories to enable them to explain the shifting phenomena at the micro and macro level for every generation. Exploratory research to better understand the characteristics of a generation in other settings is a crucial proposal proposed by this study.
Living up to your codes? Corporate codes of ethics and the cost of equity capital
Authors: Duong Hong Kim , Fasan Marco , Gotti Giorgio
Published: January 2022

Purpose

Previous literature provides mixed evidence about the effectiveness of a code of ethics in limiting managerial opportunism. While some studies find that code of ethics is merely window-dressing, others find that they do influence managers' behavior. The present study investigates whether the quality of a code of ethics decreases the cost of equity by limiting managerial opportunism.

Design/methodology/approach

In order to test the hypothesis, the authors perform an empirical analysis on a sample of US companies in the 2004–2012 period. The results are robust to a battery of robustness analyses that the authors performed in order to take care of endogeneity.

Findings

Empirical results indicate that a higher quality code of ethics is associated with a lower cost of equity. In other words, firms with a more comprehensive code of ethics and better-designed implementation procedures limit managerial opportunism and pay a lower cost of equity because they are perceived by investors to be less risky.

Research limitations/implications

Practical implications

Social implications

Originality/value

The authors contribute to the literature in two ways. First, by looking at the market reaction to the code of ethics, thus capturing all its indirect possible benefits and second, by measuring not only the existence but also the quality of a code of ethics. Based on the results, policymakers may choose to further promote codes of ethics as an effective corporate governance mechanism.
Bacterial viability, antioxidant stability, antimutagenicity and sensory properties of onion types fermentation by using probiotic starter during storage
Authors: Al-Sahlany Shayma T.G. , Niamah Alaa K.
Published: January 2022

Purpose

The purpose of this study was to assess the bacterial viability, antioxidative activity, antimutagenicity and sensory evaluation of fermented onion types by using probiotic starters after fermentation at 37 °C for 24 hours and storage in the refrigerator for 28 days.

Design/methodology/approach

For onion fermentation, Lactobacillus acidophilus (LA-5), Bifidobacterium bifidum (BB-12), and Streptococcus thermophilus (ST) were utilised. This research was conducted on three types of onion: white onion, red onion and scallion. With a 5% brine solution, the onions were sliced into 3-5 cm long and 1-2 cm wide slices. The process of fermentation was achieved by adding 2% (108 CFU/ gm) of fresh probiotic starter and incubating it for 24 hours at 37 °C. The fermented onion samples were kept in the refrigerator for 28 days. After fermentation and storage, the pH and total acidity were estimated, the vitality of probiotic bacteria was evaluated in samples of the onion species. The Gas chromatography-mass spectrometry (GC-MS) technique was used to identify the bioactive components in fermented onion types. The antioxidant activity of fermented onions was measured using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity assay and the hydroxyl radical scavenging activity test. The Ames test was used to detect the antimutagenicity of fermented onion samples.

Findings

After fermentation, the fermented scallion (p = 0.036) has the highest vitality of all the starter bacteria species. The fermentation of onion types produced a pH of between 4.1–4.7 and 0.19–0.23% total acidity, which is in the range of reduced 3.1–3.5 pH values and 0.42–0.63% total acidity after 28 days. The viability of Lactobacillus acidophilus and Bifidobacterium bifidum in fermented scallions was Log. 7.79 and 7.57 CFU/gm. The GC-MS technique found 14 bioactive compounds in fermented white onions and 13 compounds in fermented white onions, with 15 compounds in scallion fermentation. The majority of these bioactive compounds are strong antioxidants. The antioxidant properties of fermented scallion significantly increased after 28 days of storage time, showing an inhibitory effect on the DPPH assay (p = 0.02) and the scavenging activity of the hydroxyl radical assay (p = 0.01). Sensory evaluation tests revealed that the fermented scallion was a suitable product in terms of appearance, aroma and overall acceptability.

Originality/value

Commercially accessible probiotic foods account for a sizable portion of the consumer market. Furthermore, as consumer interest in healthy eating grows, so does demand for plant-based goods. All onion types fermented with probiotic bacteria have many chemical compounds that have both antioxidant and carcinogenic activity. The fermented scallion onion sample was significantly superior to the rest of the other types of onions.
The localised accounting environment in the implementation of fair value accounting in Indonesia
Authors: Nugraheni Bernadia Linggar Yekti , Cummings Lorne Stewart , Kilgore Alan
Published: January 2022

Purpose

This case study aims to investigate the role of actors in the implementation of fair value standards in an emerging country, Indonesia.

Design/methodology/approach

This study uses semi-structured interviews with important actors within the local accounting profession, standard setting and regulatory environment, to analyse fair value accounting implementation. This study also incorporates information from press releases and newspapers, to provide a more comprehensive picture of fair value implementation.

Findings

First, professionals undertake routine actions, cultivate interests and strategically navigate their environment during the process of fair value standard implementation. Second, the role of appraisers becomes more prominent during this process. Third, government involvement is significant in ensuring the successful implementation of global accounting standards.

Research limitations/implications

First, differing localised contexts, including communities and actors, may shape how an emerging country undertakes the diffusion and implementation of global standards, which in turn can also lead to institutional change. Second, government involvement is crucial in supporting the implementation of global accounting standards within emerging economies. Third, implementing market-based measurements within emerging economies characterised by a lack of an active and liquid market may present challenges.

Practical implications

Third, implementing market-based measurements within emerging economies characterised by a lack of an active and liquid market may present challenges.

Originality/value

This study applies the concept of Institutional Work within Institutional Theory to explain how fair value standards are implemented within a localised emerging economy characterised by unique actor roles and goal-directed action.
When innocence is no protection: governance failure of digitization and its impact on local level implementation
Authors: Ramadani Luthfi , Yovadiani Amalia , Dewi Fitriyana
Published: January 2022

Purpose

Governance of e-government is rarely discussed in the initial digitization stage, especially in developing countries where the government’s focus is mainly to pursue rapid proliferation of digital adoption rather than to implement governance. This study aims to explore the consequences of this absence of governance at local level conditions.

Design/methodology/approach

An in-depth exploratory case study is conducted at a municipal health government in a southern city in Kalimantan Island, Indonesia, examining the conditions of local actors in response to various nationwide health digitization imperatives. The postcolonial theory with the critical paradigm is used to interpret and conceptualize the empirical findings.

Findings

This study identifies two critical failures of digitization governance that represent the mainstream condition: horizontal sectoral ego and vertical asymmetry and misalignment. These failures have resulted in undesirable consequences at the subalterns indicated by diverse ambivalence and de-voiced constructs displayed by the local actors.

Practical implications

This paper suggests that various issues that emerge from local level implementation in nationwide digitization agenda might not always be issues of local technology adoption, but rather negative impacts due to the absence of governance practice at the strategic level.

Originality/value

Through a critical perspective, this study unearths the underlying power and structural inequity responsible for generating the various issues and undesirable consequences that emerge at local levels related to the nationwide digitization agenda.
COMBED: Rapid non‐invasive Cardiac Output Monitoring Baseline assessment in adult Emergency Department patients with haemodynamic instability
Authors: Eyeington Christopher T , Canet Emmanuel , Cutuli Salvatore L , Ancona Paolo , Brown Alistair J , Jenkins Emily , Taylor David McD , Eastwood Glenn M , Bellomo Rinaldo
Published: January 2022

Objective

The application of rapid, non‐operator‐dependent, non‐invasive cardiac output monitoring (COM) may provide early physiological information in ED patients with haemodynamic instability (HI). Our primary objective was to assess the feasibility of measuring pre‐intervention (baseline) cardiac index (CI) and associated haemodynamic parameters.

Methods

We performed a prospective observational study of adults shortly after presentation to the ED of a large university hospital with tachycardia or hypotension or both. We applied non‐invasive COM for 5 min and recorded CI, mean arterial pressure (MAP), stroke volume index (SVI) and systemic vascular resistance index (SVRI). We assessed for differences between those presenting with hypotension or hypotension and tachycardia with tachycardia alone and between those with or without suspected infection.

Results

We obtained haemodynamic parameters in 46 of 49 patients. In patients with hypotension or hypotension and tachycardia (n = 15) rather than tachycardia alone (n = 31), we observed a lower MAP (60.8 vs 87.7, P < 0.0001), CI (2.8 vs 3.9, P = 0.0167) and heart rate (85.5 vs 115.4, P < 0.0001). There was no difference in SVI (33.7 vs 33.4, P = 0.93) or SVRI (1970 vs 2088, P = 0.67). Patients with suspected infection had similar haemodynamic values except for a lower SVRI (1706 vs 2237, P = 0.011).

Conclusions

Rapid, non‐operator‐dependent, non‐invasive COM was possible in >90% of ED patients presenting with HI. Compared with tachycardia alone, patients with hypotension had lower CI, MAP and heart rate, while those with suspected infection had a lower SVRI. This technology provides novel insights into the early state of the circulation in ED patients with HI.
Retrospective study of the prevalence and characteristics of adverse drug events in adults who present to an Australian emergency department
Authors: Brown Nathan J , Doran Elizabeth , Greenslade Jaimi H , Lukin Bill , Cottrell Neil , Jaramillo Fabian , Coombes Ian , Donovan Peter , Cullen Louise
Published: January 2022

Objective

To determine the burden, on the ED, of harm from unintentional adverse drug events (ADEs) in the community.

Methods

A retrospective, observational study of 936 randomly selected presentations to a level 6 ED at a principal referral hospital in Brisbane, Australia, in November 2017. Clinical records were screened by a pharmacist, who identified suspected ADEs. All suspected ADEs and a random selection of presentations without ADEs were reviewed by an expert panel, which classified, by consensus: occurrence and type of ADE, contribution of ADE to presentation, severity of harm and preventability of presentation. Medication‐related ED presentations (ADE‐Ps) and potential ADEs were, respectively, defined as presentations directly attributable to an ADE, and medication events that occurred but did not cause the ED presentation. Descriptive data analysis was performed.

Results

The median (interquartile range) age of patients was 40 (27–58) years, with 49.7% (95% confidence interval [CI] 46.5–52.9) being male. The prevalences of ADE‐Ps and potential ADEs were 9.2% (95% CI 7.5–11.3) and 5.0% (95% CI 3.8–6.6), respectively. The severity of harm was classified as ‘death or likely permanent harm’ in 4.7% (95% CI 0.2–9.1) of ADE‐Ps, ‘temporary harm’ (89.5%, 95% CI 83.1–96.0) and ‘minimal or no harm’ (5.8%, 95% CI 0.9–10.8). Most (79.1%, 95% CI 70.5–87.7) ADE‐Ps were preventable.

Conclusions

There is a high burden on emergency care because of unintended medication harm in the community. Interventions to reduce such harm are likely to require a co‐ordinated primary, acute and public healthcare response. The high proportion of presentations with potential ADEs indicates opportunity for harm mitigation in the ED.
Socioemotional wealth, entrepreneurial behaviour and open innovation breadth in family firms: The joint effect on innovation performance
Authors: Gjergji Rafaela , Lazzarotti Valentina , Visconti Federico
Published: January 2022

Through a survey addressed to family firms in Northern Italy, we investigate the antecedents and the different outcomes of entrepreneurial behaviour (EB) in family firms. We test a model that determines how factors such as non‐economic goals/socioemotional wealth (SEW) dimensions of family managers influence EB and how EB, in turn, affects open innovation choices (i.e., breadth) and innovation performance. We assess the interplay among these variables by means of a structural equation modelling (SEM) model. As concerns the antecedents of EB, findings show a positive influence of the SEW dimensions ‘identification with the firm’ and ‘binding social ties’, whereas a negative influence emerges regarding ‘emotional attachment among family members’. Moreover, EB enhances openness breadth, and this, in turn, acting as a mediator of the relationship between EB and innovation performance, fosters explorative results.
Expanding the limits of nephron‐sparing surgery: Surgical technique and mid‐term outcomes of purely off‐clamp robotic partial nephrectomy for totally endophytic renal tumors
Authors: Tuderti Gabriele , Brassetti Aldo , Mastroianni Riccardo , Misuraca Leonardo , Bove Alfredo , Anceschi Umberto , Ferriero Mariaconsiglia , Guaglianone Salvatore , Gallucci Michele , Simone Giuseppe
Published: January 2022

Objectives

To report surgical technique, perioperative, oncological, and mid‐term functional outcomes in a single‐center purely off‐clamp robotic partial nephrectomy series for totally endophytic masses.

Methods

A retrospective analysis of a prospectively collected, institutional review board‐approved renal cancer database was carried out to include patients with a totally endophytic renal tumor treated with off‐clamp robotic partial nephrectomy between January 2013 and December 2020 at our center. Our database was queried to identify cases that had been assigned 3 points for the “E” domain of the R.E.N.A.L. nephrometry score and 3 points for the “exophytic rate” domain of the PADUA (Preoperative Aspects and Dimensions Used for an Anatomical) nephrometry score. Preoperative indocyanine green renal mass marking was performed in 33 patients, in whom the tumor was vascularized by a specific feeding artery. Surgical steps, perioperative, oncological and functional data were reported.

Results

Fifty‐six consecutive patients with totally endophytic renal masses were treated. The median tumor diameter was 3 cm, and median PADUA and R.E.N.A.L. scores were both 10. The median operative time was 82 min. Low‐grade Clavien complications occurred in two patients (3.6%) and high‐grade Clavien complications were observed in four patients (7.1%). Positive surgical margins were detected in one patient; 2‐year recurrence‐free, cancer‐specific, and overall survival rates were 100%, 100%, and 98.2%, respectively. At a median follow‐up of 24 months, new onset of chronic kidney disease stage 3b occurred in one patient. At last follow‐up, the median estimated glomerular filtration rate was 77 mL/min, with a median estimated glomerular filtration rate percent decrease of 5.5%. Trifecta was achieved in 91% of patients.

Conclusions

Purely off‐clamp robotic partial nephrectomy is a feasible and safe surgical approach, even in totally endophytic renal tumors, providing a favorable perioperative complications rate, excellent oncological outcomes, and negligible impact on renal function at mid‐term follow‐up. Indocyanine green preoperative marking of endophytic renal tumors represents a useful tool for rapid intraoperative identification of the mass, real‐time control of resection margins, and a more precise dissection.
Sexual dysfunction in people treated with long‐acting injectable antipsychotics in monotherapy or polypharmacy: a naturalistic study
Authors: Martínez‐Giner Gema , Giménez‐De Llano Elisa , Romero‐Rubio Dolores , Abad‐Pérez María José , Sánchez‐Martínez Vanessa
Published: January 2022

Sexual dysfunction, psychosis, and antipsychotics are known to be related, but the precise association between them is still unknown. Most evidence about the prevalence of sexual dysfunction in people treated with antipsychotic drugs comes from studies with restrictive samples. That is why our main objective was to determine the prevalence of sexual dysfunction in a real‐life sample of outpatients treated with antipsychotics, considering gender. A cross‐sectional naturalistic study was developed, including people treated with long‐acting injectable antipsychotics, with or without other psychotropic drugs. Participants were interviewed to assess sexual satisfaction through a Likert scale (0 to 10) and the presence of sexual dysfunction (the Psychotropic‐Related Sexual Dysfunction Questionnaire, PRSexDQ‐SALSEX). The participants also had a blood test to determine prolactin (men and women) and testosterone levels (men only). A total of 131 people participated in the study (90 men and 41 women). Some extent of sexual dysfunction was found in 62.2% of men and 51.2% of women. The most frequent sexual dysfunction symptom for both genders was the loss of libido (45%). Hyperprolactinemia was present in 56% of men and 61% of women. The presence of sexual dysfunction was associated with higher doses of antipsychotics, hyperprolactinemia, and smoking in men and with smoking and hyperprolactinemia in postmenopausal women. This study provides real‐life evidence of sexual dysfunction and hyperprolactinemia in persons treated with long‐acting injectable antipsychotics segregated by gender. The high rates of sexual dysfunction and hyperprolactinemia detected corroborate the need to consider these aspects in clinical practice.
Impact of respiratory syncytial virus disease on quality of life in adults aged ≥50 years: A qualitative patient experience cross‐sectional study
Authors: Curran Desmond , Cabrera Eliazar Sabater , Bracke Benjamin , Raymond Kimberly , Foster April , Umanzor Cindy , Goulet Philibert , Powers John H.
Published: January 2022

Background

Information about the impact of respiratory syncytial virus (RSV) on quality of life in older adults is limited. This study characterized the patient experience of RSV illness in USA older adults and assessed the content validity of the InFLUenza Patient Reported Outcome (FLU‐PRO) in this population.

Methods

This qualitative, non‐interventional, cross‐sectional study included hybrid concept elicitation and cognitive debriefing interviews with 30 individuals (age ≥50 years) with polymerase chain reaction‐confirmed RSV diagnosed within 6 months of screening. Targeted literature review was first conducted to inform the development of interview materials. Webcam or telephone interviews were conducted by qualitative researchers using a semistructured interview guide. Interview transcripts were coded and analyzed using Excel and NVivo software.

Results

All participants reported impacts on daily activities, social activities, and relationships during RSV disease. Physical functioning was impaired in 25 (83%) participants, and 18 (60%) reported not engaging in leisure activities/hobbies. All nine participants who were working reported major impacts on work. Most (n = 28; 93%) described emotional impacts. A majority (n = 19; 63%) reported symptoms lasting beyond the acute disease stage from a week to >1 month. Symptom concepts reported generally matched FLU‐PRO items and domains. Cognitive debriefing indicated that FLU‐PRO was easy to understand and captured participants' experiences of RSV illness.

Conclusions

This study indicates that RSV disease in adults aged ≥50 years in the USA has substantial impacts on daily life and that the concepts included in FLU‐PRO are appropriate and fit for purpose as a measure of RSV symptoms in this population.
Effects of inhibition of Wnt/beta‐catenin pathway on Amacrine and Muller cells in retinas of zebrafish embryos at different development stages
Authors: Yesilyurt Gozde Alkan , Cark Ozge , Bostanci Aysenur , Akdogan Gulcin Cakan , Unsal Ayse Ipek Akyuz , Ergin Kemal , Eliyatkin Nuket Ozkavruk , Ozhan Gunes
Published: January 2022

Purpose

The aim of this study was to investigate the changes of Wnt pathway inhibition on Amacrine and Muller cells in the embryonic period of zebrafish.

Methods

Zebrafishes divided into two subgroups: control and Wnt signaling pathway inhibited groups. The effect of Wnt inhibition (IWR1) on Amacrine and Müller cells were examined at 24th, 38th, 60th and 72nd hours of Zebrafish embryos. Immunofluorescence imaging and in situ hybridization methods were used to compare the ocular structures of the groups treated with glutamine synthetase, calretinin, Neuro D1 (Amacrine and Muller cell markers), Pax6 and Rx3 (genes effective in eye formation).

Results

The most striking results of our study are; the diminished overall cellular structures of neuroretina at the 24th, 60th hour with Neuro D1, decreased Phospho‐Beta‐catenin staining at the 24th hour, apoptosis of whole ocular tissues at the 24th hour with Cleaved Caspase 3, no staining with glutamine synthase at the 38th hour, reduced β‐Catenin staining in the 60th hour and increased Caspase 3 staining at 72nd hour in IWR group. The related figures (Fig 1–7) are shown below.

Conclusions

The results of our study revealed that the inhibition of the Wnt/beta‐catenin pathway could be responsible for the maldevelopment of ocular tissues especially on the neuroretinal tissues including Amacrine and Muller cells at the early stages. Our results could shed a light on the pathophysiology of developmental anomalies of ocular tissues like colobomas and be a new model for other experimental researches like retinopathies in the future.
Perceived quality of collaboration in dehydration care among Dutch nursing home professionals: A cross‐sectional study
Authors: Paulis Simone J. C. , Everink Irma H. J. , Halfens Ruud J. G. , Lohrmann Christa , Schols Jos M. G. A.
Published: January 2022

Aim

To explore the perceived quality of collaboration in dehydration care among nursing and medical staff in Dutch nursing homes.

Design

A cross‐sectional study.

Methods

An online questionnaire was administered to nursing and medical staff in February 2020 to assess the quality of collaboration in dehydration care and its influencing factors. Descriptive statistics, chi‐square tests and multinomial logistic regression analysis were used to describe the results and examine differences between groups.

Results

In total, 695 questionnaires were completed by multiple levels of (specialized) nursing staff and nursing home physicians. The quality of collaboration was assessed as good (23.2%), sufficient (59.4%) and insufficient (17.4%). Predicting factors related to perceiving the quality of collaboration as good were working experience, dehydration training during education and the presence of a dehydration protocol/guideline in the nursing home. Enabling factors related to collaboration in dehydration care were ‘availability of sufficient aids to detect dehydration’, ‘continuity in the care relationship’ and ‘sufficient background data of the resident in the care record’. Factors that hinder collaboration were ‘insufficient knowledge about dehydration among nursing and medical staff’, ‘the absence of a team meeting in which the topic dehydration is discussed’ and ‘insufficient staffing level among nursing and medical staff’.

Conclusion

Collaboration in dehydration care was generally assessed as sufficient. Participants with >10 years of working experience, who received dehydration training during their education and had a dehydration protocol/guideline available in the nursing home, perceived the quality of collaboration more often as good. Experienced barriers and enablers for collaboration in dehydration care varied between professional groups. Therefore, it is important to gain more insight into (informal) caregivers’ perceptions on what is expected from each other about dehydration care.

Impact

Care professionals experience several limiting factors in collaborating in dehydration care. Addressing these factors could optimize dehydration care in Dutch nursing homes.
Are there any color match and color correlation between maxillary anterior teeth?
Authors: Tabatabaian Farhad , Ourang Seyed AmirHossein , Khezri Amir Saleh , Namdari Mahshid
Published: January 2022

Objective

To evaluate the color match and color correlation between maxillary anterior teeth.

Materials and Methods

CIELab values of 1182 intact maxillary anterior teeth in 197 human specimens were measured through spectrophotometry. ∆E00 color differences between similar regions of the same and different type teeth were calculated and compared with perceptibility and acceptability thresholds using 1‐sample t test to evaluate color matches. Regression analyses assessed linear relationships between the color coordinates of similar regions of different type teeth. Percentages of different modes of the color match between the same specimen's teeth (2‐tooth/3‐tooth color match or color mismatch) were determined.

Results

Mean ∆E00 values for the same type teeth were less than 1.8 (p = 1). Mean ∆E00 values for different type teeth were mostly greater than 1.8 (p < 0.001), except for central and lateral teeth in middle (p = 0.29) and incisal (p = 0.75) regions and for lateral and canine teeth in cervical regions (p = 0.33). The 2‐tooth color match showed the highest percentage (>50%).

Conclusions

The same type teeth indicated color matches. Central and lateral teeth showed color matches in middle and incisal regions, while lateral and canine teeth disclosed color matches in cervical regions. The corresponding color coordinates of mismatched regions were linearly correlated.

Clinical Significance

In order to predict and determine the shade of maxillary anterior teeth and create natural colors for corresponding restorations, some tooth color relationships and equations are presented in this study.
Predicting melatonin suppression by light in humans: Unifying photoreceptor‐based equivalent daylight illuminances, spectral composition, timing and duration of light exposure
Authors: Giménez Marina C. , Stefani Oliver , Cajochen Christian , Lang Dieter , Deuring Gunnar , Schlangen Luc J. M.
Published: January 2022

Light‐induced melatonin suppression data from 29 peer‐reviewed publications was analysed by means of a machine‐learning approach to establish which light exposure characteristics (ie photopic illuminance, five α‐opic equivalent daylight illuminances [EDIs], duration and timing of the light exposure, and the dichotomous variables pharmacological pupil dilation and narrowband light source) are the main determinants of melatonin suppression. Melatonin suppression in the data set was dominated by four light exposure characteristics: (1) melanopic EDI, (2) light exposure duration, (3) pupil dilation and (4) S‐cone‐opic EDI. A logistic model was used to evaluate the influence of each of these parameters on the melatonin suppression response. The final logistic model was only based on the first three parameters, since melanopic EDI was the best single (photoreceptor) predictor that was only outperformed by S‐cone‐opic EDI for (photopic) illuminances below 21 lux. This confirms and extends findings on the importance of the metric melanopic EDI for predicting biological effects of light in integrative (human‐centric) lighting applications. The model provides initial and general guidance to lighting practitioners on how to combine spectrum, duration and amount of light exposure when controlling non‐visual responses to light, especially melatonin suppression. The model is a starting tool for developing hypotheses on photoreceptors’ contributions to light's non‐visual responses and helps identifying areas where more data are needed, like on the S‐cone contribution at low illuminances.