bluuebunny/crossref_metadata_2025 huggingface dataset:
MCCRNet: A Multi-Level Change Contextual Refinement Network for Remote Sensing Image Change Detection
Authors: Ke Qingtian , Zhang Peng
Published: September 2021

Change detection based on bi-temporal remote sensing images has made significant progress in recent years, aiming to identify the changed and unchanged pixels between a registered pair of images. However, most learning-based change detection methods only utilize fused high-level features from the feature encoder and thus miss the detailed representations that low-level feature pairs contain. Here we propose a multi-level change contextual refinement network (MCCRNet) to strengthen the multi-level change representations of feature pairs. To effectively capture the dependencies of feature pairs while avoiding fusing them, our atrous spatial pyramid cross attention (ASPCA) module introduces a crossed spatial attention module and a crossed channel attention module to emphasize the position importance and channel importance of each feature while simultaneously keeping the scale of input and output the same. This module can be plugged into any feature extraction layer of a Siamese change detection network. Furthermore, we propose a change contextual representations (CCR) module from the perspective of the relationship between the change pixels and the contextual representation, named change region contextual representations. The CCR module aims to correct changed pixels mistakenly predicted as unchanged by a class attention mechanism. Finally, we introduce an effective sample number adaptively weighted loss to solve the class-imbalanced problem of change detection datasets. On the whole, compared with other attention modules that only use fused features from the highest feature pairs, our method can capture the multi-level spatial, channel, and class context of change discrimination information. The experiments are performed with four public change detection datasets of various image resolutions. Compared to state-of-the-art methods, our MCCRNet achieved superior performance on all datasets (i.e., LEVIR, Season-Varying Change Detection Dataset, Google Data GZ, and DSIFN) with improvements of 0.47%, 0.11%, 2.62%, and 3.99%, respectively.
Role of Brown and Beige Adipose Tissues in Seasonal Adaptation in the Raccoon Dog (Nyctereutes procyonoides)
Authors: Niiranen Laura , Mäkelä Kari A. , Mutt Shivaprakash J. , Viitanen Riikka , Kaisanlahti Anna , Vicente David , Noponen Tommi , Autio Anu , Roivainen Anne , Nuutila Pirjo , Saarela Seppo , Herzig Karl-Heinz
Published: September 2021

Brown adipose tissue (BAT) expresses uncoupling protein-1 (UCP1), which enables energy to be exerted towards needed thermogenesis. Beige adipocytes are precursor cells interspersed among white adipose tissue (WAT) that possess similar UCP1 activity and capacity for thermogenesis. The raccoon dog (Nyctereutes procyonoides) is a canid species that utilizes seasonal obesity to survive periods of food shortage in climate zones with cold winters. The potential to recruit a part of the abundant WAT storages as beige adipocytes for UCP1-dependent thermogenesis was investigated in vitro by treating raccoon dog adipocytes with different browning inducing factors. In vivo positron emission tomography/computed tomography (PET/CT) imaging with the glucose analog 18F-FDG showed that BAT was not detected in the adult raccoon dog during the winter season. In addition, UCP1 expression was not changed in response to chronic treatments with browning inducing factors in adipocyte cultures. Our results demonstrated that most likely the raccoon dog endures cold weather without the induction of BAT or recruitment of beige adipocytes for heat production. Its thick fur coat, insulating fat, and muscle shivering seem to provide the adequate heat needed for surviving the winter.
De Novo Missense Mutations in TNNC1 and TNNI3 Causing Severe Infantile Cardiomyopathy Affect Myofilament Structure and Function and Are Modulated by Troponin Targeting Agents
Authors: Hassoun Roua , Budde Heidi , Mannherz Hans Georg , Lódi Mária , Fujita-Becker Setsuko , Laser Kai Thorsten , Gärtner Anna , Klingel Karin , Möhner Desirée , Stehle Robert , Sultana Innas , Schaaf Thomas , Majchrzak Mario , Krause Verena , Herrmann Christian , Nowaczyk Marc M. , Mügge Andreas , Pfitzer Gabriele , Schröder Rasmus R. , Hamdani Nazha , Milting Hendrik , Jaquet Kornelia , Cimiotti Diana
Published: September 2021

Rare pediatric non-compaction and restrictive cardiomyopathy are usually associated with a rapid and severe disease progression. While the non-compaction phenotype is characterized by structural defects and is correlated with systolic dysfunction, the restrictive phenotype exhibits diastolic dysfunction. The molecular mechanisms are poorly understood. Target genes encode among others, the cardiac troponin subunits forming the main regulatory protein complex of the thin filament for muscle contraction. Here, we compare the molecular effects of two infantile de novo point mutations in TNNC1 (p.cTnC-G34S) and TNNI3 (p.cTnI-D127Y) leading to severe non-compaction and restrictive phenotypes, respectively. We used skinned cardiomyocytes, skinned fibers, and reconstituted thin filaments to measure the impact of the mutations on contractile function. We investigated the interaction of these troponin variants with actin and their inter-subunit interactions, as well as the structural integrity of reconstituted thin filaments. Both mutations exhibited similar functional and structural impairments, though the patients developed different phenotypes. Furthermore, the protein quality control system was affected, as shown for TnC-G34S using patient’s myocardial tissue samples. The two troponin targeting agents levosimendan and green tea extract (-)-epigallocatechin-3-gallate (EGCg) stabilized the structural integrity of reconstituted thin filaments and ameliorated contractile function in vitro in some, but not all, aspects to a similar degree for both mutations.
Fe-N Co-Doped Titanium Dioxide Nanoparticles Induce Cell Death in Human Lung Fibroblasts in a p53-Independent Manner
Authors: Nica Ionela Cristina , Stan Miruna S. , Popescu Roua G. , Nicula Nicoleta , Ducu Robert , Diamandescu Lucian , Dinischiotu Anca
Published: September 2021

The advancement of nanotechnology in the last decade has developed an abundance of novel and intriguing TiO2-based nanomaterials that are widely used in many sectors, including industry (as a food additive and colorant in cosmetics, paints, plastics, and toothpaste) and biomedicine (photoelectrochemical biosensing, implant coatings, drug delivery, and new emerging antimicrobial agents). Therefore, the increased use of engineered nanomaterials in the industry has raised serious concern about human exposure and their unexpected cytotoxic effects. Since inhalation is considered the most relevant way of absorbing nanomaterials, different cell death mechanisms induced in MRC-5 lung fibroblasts, following the exposure to functionalized TiO2 NPs, were investigated. Long-term exposure to TiO2 nanoparticles co-doped with 1% of iron and nitrogen led to the alteration of p53 protein activity and the gene expression controlled by this suppressor (NF-kB and mdm2), DNA damage, cell cycle disruptions at the G2/M and S phases, and lysosomal membrane permeabilization and the subsequent release of cathepsin B, triggering the intrinsic pathway of apoptosis in a Bax- and p53-independent manner. Our results are of major significance, contributing to the understanding of the mechanisms underlying the interaction of these nanoparticles with in vitro biological systems, and also providing useful information for the development of new photocatalytic nanoparticles that are active in the visible spectrum, but with increased biocompatibility.
Targeted Disruption of the Inhibitor of DNA Binding 4 (Id4) Gene Alters Photic Entrainment of the Circadian Clock
Authors: Duffield Giles E. , Robles-Murguia Maricela , Hou Tim Y. , McDonald Kathleen A.
Published: September 2021

Inhibitor of DNA binding (Id) genes comprise a family of four helix–loop–helix (HLH) transcriptional inhibitors. Our earlier studies revealed a role for ID2 within the circadian system, contributing to input, output, and core clock function through its interaction with CLOCK and BMAL1. Here, we explore the contribution of ID4 to the circadian system using a targeted disruption of the Id4 gene. Attributes of the circadian clock were assessed by monitoring the locomotor activity of Id4−/− mice, and they revealed disturbances in its operation. Id4-mutant mice expressed a shorter circadian period length, attenuated phase shifts in responses to continuous and discrete photic cues, and an advanced phase angle of entrainment under a 12:12 light:dark cycle and under short and long photoperiods. To understand the basis for these properties, suprachiasmatic nucleus (SCN) and retinal structures were examined. Anatomical analysis reveals a smaller Id4−/− SCN in the width dimension, which is a finding consistent with its smaller brain. As a result of this feature, anterograde tracing in Id4−/− mice revealed retinal afferents innovate a disproportionally larger SCN area. The Id4−/− photic entrainment responses are unlikely to be due to an impaired function of the retinal pathways since Id4−/− retinal anatomy and function tested by pupillometry were similar to wild-type mice. Furthermore, these circadian characteristics are opposite to those exhibited by the Id2−/− mouse, suggesting an opposing influence of the ID4 protein within the circadian system; or, the absence of ID4 results in changes in the expression or activity of other members of the Id gene family. Expression analysis of the Id genes within the Id4−/− SCN revealed a time-of-day specific elevated Id1. It is plausible that the increased Id1 and/or absence of ID4 result in changes in interactions with bHLH canonical clock components or with targets upstream and/or downstream of the clock, thereby resulting in abnormal properties of the circadian clock and its entrainment.
Aggregation Condition–Structure Relationship of Mouse Prion Protein Fibrils
Authors: Fridmanis Jēkabs , Toleikis Zigmantas , Sneideris Tomas , Ziaunys Mantas , Bobrovs Raitis , Smirnovas Vytautas , Jaudzems Kristaps
Published: September 2021

Prion diseases are associated with conformational conversion of cellular prion protein into a misfolded pathogenic form, which resembles many properties of amyloid fibrils. The same prion protein sequence can misfold into different conformations, which are responsible for variations in prion disease phenotypes (prion strains). In this work, we use atomic force microscopy, FTIR spectroscopy and magic-angle spinning NMR to devise structural models of mouse prion protein fibrils prepared in three different denaturing conditions. We find that the fibril core region as well as the structure of its N- and C-terminal parts is almost identical between the three fibrils. In contrast, the central part differs in length of β-strands and the arrangement of charged residues. We propose that the denaturant ionic strength plays a major role in determining the structure of fibrils obtained in a particular condition by stabilizing fibril core interior-facing glutamic acid residues.
Fabrication of Hydroxyapatite with Bioglass Nanocomposite for Human Wharton’s-Jelly-Derived Mesenchymal Stem Cell Growing Substrate
Authors: Ebrahimi Shamsi , Hanim Yusoff Umul , Sipaut Coswald Stephen , Jan Norsazlina binti Ahmad , Arshad Sazmal E. , How Siew Eng
Published: September 2021

Recently, composite scaffolding has found many applications in hard tissue engineering due to a number of desirable features. In this present study, hydroxyapatite/bioglass (HAp/BG) nanocomposite scaffolds were prepared in different ratios using a hydrothermal approach. The aim of this research was to evaluate the adhesion, growth, viability, and osteoblast differentiation behavior of human Wharton’s-jelly-derived mesenchymal stem cells (hWJMSCs) on HAp/BG in vitro as a scaffold for application in bone tissue engineering. Particle size and morphology were investigated by TEM and bioactivity was assessed and proven using SEM analysis with hWJMSCs in contact with the HAp/BG nanocomposite. Viability was evaluated using PrestoBlueTM assay and early osteoblast differentiation and mineralization behaviors were investigated by ALP activity and EDX analysis simultaneously. TEM results showed that the prepared HAp/BG nanocomposite had dimensions of less than 40 nm. The morphology of hWJMSCs showed a fibroblast-like shape, with a clear filopodia structure. The viability of hWJMSCs was highest for the HAp/BG nanocomposite with a 70:30 ratio of HAp to BG (HAp70/BG30). The in vitro biological results confirmed that HAp/BG composite was not cytotoxic. It was also observed that the biological performance of HAp70/BG30 was higher than HAp scaffold alone. In summary, HAp/BG scaffold combined with mesenchymal stem cells showed significant potential for bone repair applications in tissue engineering.
Entropy-Enthalpy Compensations Fold Proteins in Precise Ways
Authors: Li Jiacheng , Hou Chengyu , Ma Xiaoliang , Guo Shuai , Zhang Hongchi , Shi Liping , Liao Chenchen , Zheng Bing , Ye Lin , Yang Lin , He Xiaodong
Published: September 2021

Exploring the protein-folding problem has been a longstanding challenge in molecular biology and biophysics. Intramolecular hydrogen (H)-bonds play an extremely important role in stabilizing protein structures. To form these intramolecular H-bonds, nascent unfolded polypeptide chains need to escape from hydrogen bonding with surrounding polar water molecules under the solution conditions that require entropy-enthalpy compensations, according to the Gibbs free energy equation and the change in enthalpy. Here, by analyzing the spatial layout of the side-chains of amino acid residues in experimentally determined protein structures, we reveal a protein-folding mechanism based on the entropy-enthalpy compensations that initially driven by laterally hydrophobic collapse among the side-chains of adjacent residues in the sequences of unfolded protein chains. This hydrophobic collapse promotes the formation of the H-bonds within the polypeptide backbone structures through the entropy-enthalpy compensation mechanism, enabling secondary structures and tertiary structures to fold reproducibly following explicit physical folding codes and forces. The temperature dependence of protein folding is thus attributed to the environment dependence of the conformational Gibbs free energy equation. The folding codes and forces in the amino acid sequence that dictate the formation of β-strands and α-helices can be deciphered with great accuracy through evaluation of the hydrophobic interactions among neighboring side-chains of an unfolded polypeptide from a β-strand-like thermodynamic metastable state. The folding of protein quaternary structures is found to be guided by the entropy-enthalpy compensations in between the docking sites of protein subunits according to the Gibbs free energy equation that is verified by bioinformatics analyses of a dozen structures of dimers. Protein folding is therefore guided by multistage entropy-enthalpy compensations of the system of polypeptide chains and water molecules under the solution conditions.
14,15-EET Reduced Brain Injury from Cerebral Ischemia and Reperfusion via Suppressing Neuronal Parthanatos
Authors: Zhao Haipeng , Tang Jing , Chen Hongyang , Gu Wei , Geng Huixia , Wang Lai , Wang Yanming
Published: September 2021

To investigate the effect of 14,15-EET on the parthanatos in neurons induced by cerebral ischemia and reperfusion, middle cerebral artery occlusion and reperfusion (MCAO/R) and oxygen glucose deprivation/reoxygenation (OGD/R) were used to simulate cerebral ischemia reperfusion in vivo and in vitro, respectively. TTC staining and the Tunel method were used to detect cerebral infarct volume and neuronal apoptosis. Western blot and immunofluorescence were used to detect poly (ADP-ribose) polymerase-1 (PARP-1) activation and AIF nuclear translocation. The production of reactive oxygen species (ROS) and the expression of antioxidant genes were detected by Mito SOX, DCFH-DA and qPCR methods. MCAO/R increased cerebral infarct volume and neuronal apoptosis in mice, while 14,15-EET pretreatment increased cerebral infarct volume and neuronal apoptosis. OGD/R induced reactive oxygen species generation, PARP-1 cleavage, and AIF nuclear translocation in cortical neurons. 14,15-EET pretreatment could enhance the antioxidant gene expression of glutathione peroxidase (GSH-Px), heme oxygenase-1 (HO-1) and superoxide dismutase (SOD) in cortical neurons after ischemia and reperfusion. 14,15-EET inhibits the neuronal parthanatos induced by MCAO/R through upregulation of the expression of antioxidant genes and by reducing the generation of reactive oxygen species. This study advances the EET neuroprotection theory and provides a scientific basis for targeted clinical drugs that reduce neuronal parthanatos following cerebral ischemia and reperfusion.
Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
Authors: Konovalova Julia , Gerasymchuk Dmytro , Arroyo Sergio Navarette , Kluske Sven , Mastroianni Francesca , Pereyra Alba Vargas , Domanskyi Andrii
Published: September 2021

Mesencephalic astrocyte derived neurotrophic factor (MANF) and cerebral dopamine neurotrophic factor (CDNF) are novel evolutionary conserved trophic factors, which exhibit cytoprotective activity via negative regulation of unfolded protein response (UPR) and inflammation. Despite multiple reports demonstrating detrimental effect of MANF/CDNF downregulation, little is known about the control of their expression. miRNAs—small non-coding RNAs—are important regulators of gene expression. Their dysregulation was demonstrated in multiple pathological processes and their ability to modulate levels of other neurotrophic factors, glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF), was previously reported. Here, for the first time we demonstrated direct regulation of MANF and CDNF by miRNAs. Using bioinformatic tools, reporter assay and analysis of endogenous MANF and CDNF, we identified that miR-144 controls MANF expression, and miR-134 and miR-141 downregulate CDNF levels. We also demonstrated that this effect is human-specific and is executed via predicted binding sites of corresponding miRNAs. Finally, we found that miR-382 suppressed hCDNF expression indirectly. In conclusion, we demonstrate for the first time direct regulation of MANF and CDNF expression by specific miRNAs, despite the fact their binding sites are not strongly evolutionary conserved. Furthermore, we demonstrate a functional effect of miR-144 mediated regulation of MANF on ER stress response markers. These findings emphasize that (1) prediction of miRNA targets based on evolutionary conservation may miss biologically meaningful regulatory pairs; and (2) interpretation of miRNA regulatory effects in animal models should be cautiously validated.
CANet: A Combined Attention Network for Remote Sensing Image Change Detection
Authors: Lu Di , Wang Liejun , Cheng Shuli , Li Yongming , Du Anyu
Published: September 2021

Change detection (CD) is one of the essential tasks in remote sensing image processing and analysis. Remote sensing CD is a process of determining and evaluating changes in various surface objects over time. The impressive achievements of deep learning in image processing and computer vision provide an innovative concept for the task of CD. However, existing methods based on deep learning still have problems detecting small changed regions correctly and distinguishing the boundaries of the changed regions. To solve the above shortcomings and improve the efficiency of CD networks, inspired by the fact that an attention mechanism can refine features effectively, we propose an attention-based network for remote sensing CD, which has two important components: an asymmetric convolution block (ACB) and a combined attention mechanism. First, the proposed method extracts the features of bi-temporal images, which contain two parallel encoders with shared weights and structures. Then, the feature maps are fed into the combined attention module to reconstruct the change maps and obtain refined feature maps. The proposed CANet is evaluated on the two publicly available datasets for challenging remote sensing image CD. Extensive empirical results with four popular metrics show that the designed framework yields a robust CD detector with good generalization performance. In the CDD and LEVIR-CD datasets, the F1 values of the CANet are 3.3% and 1.3% higher than those of advanced CD methods, respectively. A quantitative analysis and qualitative comparison indicate that our method outperforms competitive baselines in terms of both effectiveness and robustness.
Uncertainty Estimation for Machine Learning Models in Multiphase Flow Applications
Authors: Frau Luca , Susto Gian Antonio , Barbariol Tommaso , Feltresi Enrico
Published: September 2021

In oil and gas production, it is essential to monitor some performance indicators that are related to the composition of the extracted mixture, such as the liquid and gas content of the flow. These indicators cannot be directly measured and must be inferred with other measurements by using soft sensor approaches that model the target quantity. For the purpose of production monitoring, point estimation alone is not enough, and a confidence interval is required in order to assess the uncertainty in the provided measure. Decisions based on these estimations can have a large impact on production costs; therefore, providing a quantification of uncertainty can help operators make the most correct choices. This paper focuses on the estimation of the performance indicator called the water-in-liquid ratio by using data-driven tools: firstly, anomaly detection techniques are employed to find data that can alter the performance of the subsequent model; then, different machine learning models, such as Gaussian processes, random forests, linear local forests, and neural networks, are tested and employed to perform uncertainty-aware predictions on data coming from an industrial tool, the multiphase flow meter, which collects multiple signals from the flow mixture. The reported results show the differences between the discussed approaches and the advantages of the uncertainty estimation; in particular, they show that methods such as the Gaussian process and linear local forest are capable of reaching competitive performance in terms of both RMSE (1.9–2.1) and estimated uncertainty (1.6–2.6).
Rock Strain Prediction Using Deep Neural Network and Hybrid Models of ANFIS and Meta-Heuristic Optimization Algorithms
Authors: Pradeep T. , Bardhan Abidhan , Burman Avijit , Samui Pijush
Published: September 2021

The majority of natural ground vibrations are caused by the release of strain energy accumulated in the rock strata. The strain reacts to the formation of crack patterns and rock stratum failure. Rock strain prediction is one of the significant works for the assessment of the failure of rock material. The purpose of this paper is to investigate the development of a new strain prediction approach in rock samples utilizing deep neural network (DNN) and hybrid ANFIS (adaptive neuro-fuzzy inference system) models. Four optimization algorithms, namely particle swarm optimization (PSO), Fireflies algorithm (FF), genetic algorithm (GA), and grey wolf optimizer (GWO), were used to optimize the learning parameters of ANFIS and ANFIS-PSO, ANFIS-FF, ANFIS-GA, and ANFIS-GWO were constructed. For this purpose, the necessary datasets were obtained from an experimental setup of an unconfined compression test of rocks in lateral and longitudinal directions. Various statistical parameters were used to investigate the accuracy of the proposed prediction models. In addition, rank analysis was performed to select the most robust model for accurate rock sample prediction. Based on the experimental results, the constructed DNN is very potential to be a new alternative to assist engineers to estimate the rock strain in the design phase of many engineering projects.
Impact of the 18F-FDG-PET/MRI on Metastatic Staging in Patients with Hepatocellular Carcinoma: Initial Results from 104 Patients
Authors: Vermersch Mathilde , Mulé Sébastien , Chalaye Julia , Galletto Pregliasco Athena , Emsen Berivan , Amaddeo Giuliana , Monnet Aurélien , Stemmer Alto , Baranes Laurence , Laurent Alexis , Leroy Vincent , Itti Emmanuel , Luciani Alain
Published: September 2021

Optimal HCC therapeutic management relies on accurate tumor staging. Our aim was to assess the impact of 18F-FDG-WB-PET/MRI on HCC metastatic staging, compared with the standard of care CT-CAP/liver MRI combination, in patients with HCC referred on a curative intent or before transarterial radioembolization. One hundred and four consecutive patients followed for HCC were retrospectively included. The WB-PET/MRI was compared with the standard of care CT-CAP/liver MRI combination for HCC metastatic staging, with pathology, followup, and multidisciplinary board assessment as a reference standard. Thirty metastases were identified within 14 metastatic sites in 11 patients. The sensitivity of WB-PET/MRI for metastatic sites and metastatic patients was significantly higher than that of the CT-CAP/liver MRI combination (respectively 100% vs. 43%, p = 0.002; and 100% vs. 45%, p = 0.01). Metastatic sites missed by CT-CAP were bone (n = 5) and distant lymph node (n = 3) in BCLC C patients. For the remaining 93 nonmetastatic patients, three BCLC A patients identified as potentially metastatic on the CT-CAP/liver MRI combination were correctly ruled out with the WB-PET/MRI without significant increase in specificity (100% vs. 97%; p = 0.25). The WB-PET/MRI may improve HCC metastatic staging and could be performed as a “one-stop-shop” examination for HCC staging with a significant impact on therapeutic management in about 10% of patients especially in locally advanced HCC.
Psychometric Validation of the Cardiff Wound Impact Schedule Questionnaire in a Spanish Population with Diabetic Foot Ulcer
Authors: Granado-Casas Minerva , Martinez-Gonzalez Dolores , Martínez-Alonso Montserrat , Dòria Montserrat , Alcubierre Nuria , Valls Joan , Julve Josep , Verdú-Soriano José , Mauricio Didac
Published: September 2021

Diabetic foot ulcers (DFU) negatively affect the quality of life (QoL) of people with diabetes. The Cardiff Wound Impact Schedule (CWIS) questionnaire has been designed to measure the QoL of people with chronic foot wounds. However, no studies have been specifically designed to validate this instrument in a Spanish population. In this prospective study, a total of 141 subjects with DFU were recruited. DFU was determined by performing physical examinations. Medical records were exhaustively reviewed to collect clinical variables. The CWIS was transculturally adapted by a group of experts and a group of patients with DFU. The SF-36 and EQ-5D generic instruments were used as reference tools. The questionnaires were administered at 7 days and 4, 12, and 26 weeks after the baseline assessment by personal interview with each of the study subjects. The psychometric properties of the instrument were assessed using statistical methods. The content validity had an average of 3.63 (90.7% of the maximum score of 4). The internal consistency of the CWIS subscales had a standardized Cronbach’s alpha range from 0.715 to 0.797. The reproducibility was moderate with an intraclass correlation coefficient (ICC) range from 0.606 to 0.868. Significant correlations between CWIS domains and SF-36 and EQ-5D subscales were observed, demonstrating a good criterion validity of the CWIS questionnaire (p < 0.001). However, the construct validity of the CWIS was not validated with a comparative fit index (CFI) of 0.69, a root mean square error of approximation (RMSEA) of 0.09, and a standardized root mean square residual (SRMR) of 0.10. The sensitivity to changes over time was optimal in the three domains (i.e., social life, well-being, and physical symptoms) (p < 0.001). In conclusion, the Spanish version of the CWIS shows acceptable psychometric properties to assess the QoL of subjects with DFU, except for its construct validity.
Portomesenteric Vein Thrombosis after Bariatric Surgery: An Online Survey
Authors: Carrano Francesco Maria , Weiner Sylvia , Elshafei Moustafa , Ahmed Saleem , Talishinskiy Toghrul , Tognoni Valeria , Mahawar Kamal , Di Lorenzo Nicola
Published: September 2021

Portomesenteric vein thrombosis (PMVT) is a rare post-operative complication of bariatric procedures, occurring in between 0.3% and 1% of cases. A structured questionnaire consisting of 27 items was available online to members of the International Federation for the Surgery of Obesity and Metabolic Disorders (IFSO) to investigate the occurrence of PMVT. A total of 89 bariatric surgeons from 61 countries participated. Twenty-six (29.21%) reported at least one case of PMVT (46.15% males; 53.84% females). The surgery most associated with PMVT occurrence was sleeve gastrectomy (84.6%), followed by Roux-en-Y gastric bypass (RYGB) (7.69%), and laparoscopic adjustable gastric banding (LAGB) (7.69%). The time gap between surgery and PMVT was 19.28 ± 8.72 days. The predominant symptom was abdominal pain in 96.15% of patients, followed by fever in 26.9%. Complete occlusion of the portal vein was reported in 34.6% of cases, with involvement of the portal system in 69%, extension to the superior mesenteric district in 23%, and extension to the splenic vein in two patients (7.7%). Our survey, which is the largest regarding PMVT to date, revealed a diffuse lack of standardization in the choice, duration, and dosing of prophylaxis regimens as well as treatment modalities, reflecting the literature gap on the topic.
The Relationship between Serum Uric Acid and Ejection Fraction of the Left Ventricle
Authors: Ivan Vlad-Sabin , Buzas Roxana , Cuțina Morgovan Adina-Flavia , Ciubotaru Paul , Ardelean Melania , Goje Daniel , Roșca Ciprian , Timar Romulus , Lighezan Daniel
Published: September 2021

Study basis: As a byproduct of protein metabolism, serum uric acid is a controversial risk factor and is the focus of several recent studies in the field of cardiovascular disease. Whether serum uric acid is involved in the development of these pathologies alone or in conjunction with other factors is a matter of debate. Objective: The objective of this study is to assess the direct relationship between serum uric acid and the ejection fraction. Methods: A retrospective study of 303 patients with heart failure, classified according to the ESC guidelines, was conducted, and several parameters, along with the relationship between serum uric acid and ejection fraction, were characterized. Results: A direct relationship between the level of serum uric acid and the ejection fraction was established (p = 0.03); patients with higher uric acid had an increased risk of having a lower ejection fraction. Conclusions: Serum uric acid, even when asymptomatic, is linked with the level of the ejection fraction of the left ventricle.
Kidney Microcirculation as a Target for Innovative Therapies in AKI
Authors: Ergin Bülent , Akin Sakir , Ince Can
Published: September 2021

Acute kidney injury (AKI) is a serious multifactorial conditions accompanied by the loss of function and damage. The renal microcirculation plays a crucial role in maintaining the kidney’s functional and structural integrity for oxygen and nutrient supply and waste product removal. However, alterations in microcirculation and oxygenation due to renal perfusion defects, hypoxia, renal tubular, and endothelial damage can result in AKI and the loss of renal function regardless of systemic hemodynamic changes. The unique structural organization of the renal microvasculature and the presence of autoregulation make it difficult to understand the mechanisms and the occurrence of AKI following disorders such as septic, hemorrhagic, or cardiogenic shock; ischemia/reperfusion; chronic heart failure; cardiorenal syndrome; and hemodilution. In this review, we describe the organization of microcirculation, autoregulation, and pathophysiological alterations leading to AKI. We then suggest innovative therapies focused on the protection of the renal microcirculation and oxygenation to prevent AKI.
Single Surgeon Experience with 500 Cases of the Robotic Bilateral Axillary Breast Approach (BABA) for Thyroid Surgery Using the Da-Vinci Xi System
Authors: Choi Yun-Suk , Shin Woo-Young , Yi Jin-Wook
Published: September 2021

Objectives: Robotic bilateral axillary breast approach (BABA) thyroid surgery began in 2008 and is now one of the most widely used remote-access thyroid surgeries worldwide. This study aimed to analyze the results of 500 robotic BABA thyroid surgeries performed in a single institution in Korea compared with open thyroid surgery. Methods: From December 2018 to March 2020, 502 robotic BABA thyroidectomies (RTs) and 531 conventional open thyroidectomies (OTs) were performed in our institution by a single endocrine surgeon. We retrospectively reviewed patient medical records and performed a comparative analysis of OT and RT. Results: The RT group was younger (43.41 ± 11.41 versus 54.28 ± 13.41 years, p < 0.001) and had a higher proportion of females (84.3% versus 69.3%, p < 0.001), a lower BMI (24.66 ± 3.97 versus 25.83 ± 4.07 kg/m2), a higher proportion of lobectomies (52.6% versus 45.2%) and a lower proportion of lateral neck dissections (3.4% versus 10.0%, p < 0.001). The RT group had a longer operation time (145.33 ± 40.80 versus 93.39 ± 43.55 min, p < 0.001) and higher surgical costs. Although the OT group had a larger tumor size and a higher proportion of extrathyroidal extension, the numbers of retrieved lymph nodes were not significantly different between the two groups. Additionally, there was no difference in the stimulated thyroglobulin level before radioactive iodine therapy (7.01 ± 35.73 versus 8.39 ± 58.77, p = 0.782). The rates of transient vocal cord palsy and transient hypoparathyroidism were significantly lower in the RT group, and those of scar-related complications were higher in the OT group. Conclusions: Robotic BABA thyroid surgery has advantages not only in better cosmetic outcomes but also in lower rates of vocal cord palsy and hypoparathyroidism, with comparable lymph node retrieval and serum thyroglobulin levels.
Collagen XII Deficiency Increases the Risk of Anterior Cruciate Ligament Injury in Mice
Authors: Fukusato Shin , Nagao Masashi , Fujihara Kei , Yoneda Taiju , Arai Kiyotaka , Koch Manuel , Kaneko Kazuo , Ishijima Muneaki , Izu Yayoi
Published: September 2021

Anterior cruciate ligament (ACL) rupture is a common knee injury for athletes. Although surgical reconstruction is recommended for the treatment of ACL ruptures, 100% functional recovery is unlikely. Therefore, the discovery of risk factors for ACL ruptures may prevent injury. Several studies have reported an association between polymorphisms of the collagen XII gene COL12A1 and ACL rupture. Collagen XII is highly expressed in tendons and ligaments and regulates tissue structure and mechanical property. Therefore, we hypothesized that collagen XII deficiency may cause ACL injury. To elucidate the influence of collagen XII deficiency on ACL, we analyzed a mouse model deficient for Col12a1. Four- to 19-week-old male Col12a1-/- and wild-type control mice were used for gait analysis; histological and immunofluorescent analysis of collagen XII, and real-time RT-PCR evaluation of Col12a1 mRNA expression. The Col12a1-/- mice showed an abnormal gait with an approximately 2.7-fold increase in step angle, suggesting altered step alignment. Col12a1-/- mice displayed 20–60% ACL discontinuities, but 0% discontinuity in the posterior cruciate ligament. No discontinuities in knee ligaments were found in wild-type mice. Collagen XII mRNA expression in the ACL tended to decrease with aging. Our study demonstrates for the first time that collagen XII deficiency increases the risk of ACL injury.
Error Analysis of Non-Destructive Ultrasonic Testing of Glass Fiber-Reinforced Polymer Hull Plates
Authors: Han Zhiqiang , Jang Jaewon , Lee Sang-Gyu , Lee Dongkun , Oh Daekyun
Published: September 2021

Glass fiber-reinforced polymer (GFRP) ship structures are generally fabricated by hand lay-up; thus, the environmental factors and worker proficiency influence the fabrication process and presence of error in the non-destructive evaluation results. In this study, the ultrasonic testing of GFRP hull plate prototypes was conducted to investigate the statistical significance of the influences of the design parameters, e.g., the glass fiber weight fraction (Gc) and thickness variations, on the measurement error. The GFRP hull plate prototypes were fitted with E-glass fiber chopped strand mats (40 wt % content) with different thicknesses (7.72 mm, 14.63 mm, and 18.24 mm). The errors in the thickness measurements were investigated by conducting pulse-echo ultrasonic A-scan. The thickness variation resulted in increased error. Furthermore, hull plate burn-off tests were conducted to investigate the fabrication qualities. Defects such as voids did not have a significant influence on the results. The statistical analysis of the measurement errors confirmed that the thickness variations resulted in a strong ultrasonic interference between the hull plates, although the hull plates had similar specific gravity values. Therefore, the ultrasonic interference of the layer group interface should be considered to decrease the GFRP hull NDE errors with respect to an increase in the thickness and Gc.
Prediction of Bone Mineral Density (BMD) Adaptation in Pelvis–Femur Model with Hip Arthroplasties
Authors: Abdullah Abdul Halim , Todo Mitsugu
Published: September 2021

The prediction of bone remodeling behaviour is a challenging factor in encouraging the long-term stability of hip arthroplasties. The presence of femoral components modifies the biomechanical environment of the bone and alters the bone growth process. Issues of bone loss and gait instability on both limbs are associated with the remodeling process. In this study, finite element analysis with an adaptive bone remodeling algorithm was used to predict the changes in bone mineral density following total hip and resurfacing hip arthroplasty. A three-dimensional model of the pelvis–femur was constructed from computed tomography (CT-based) images of a 79-year-old female patient with hip osteoarthritis. The prosthesis stem of the total hip arthroplasty was modelled with a titanium alloy material, while the femoral head had alumina properties. Meanwhile, resurfacing of the hip implant was completed with a cobalt-chromium material. Contact between the components and bone was designed to be perfectly bonded at the interface. Results indicate that the bone mineral density was modified over five years on all models, including hip osteoarthritis. The changes of BMD were predicted as being high between year zero and year one, especially in the proximal region. Changes were observed to be minimal in the following years. The bone remodeling process was also predicted for the non-operated femur. However, the adaptation was lower compared to the operated limbs. The reduction in bone mineral density suggested the bone loss phenomenon after a few years.
Comparative Modelling and Thermal Analysis of AlGaN/GaN Power Devices
Authors: Manandhar Mahesh B. , Matin Mohammad A.
Published: September 2021

The use of Aluminum Gallium Nitride (AlGaN) as a power switching device material has been a promising topic of research in recent years. Along with Silicon Carbide (SiC) and Gallium Nitride (GaN), AlGaN is categorized as a Wideband Gap (WBG) material with intrinsic properties best suited for high power switching applications. This paper simulates and compares the thermal and electrical performance of AlGaN and Silicon (Si) MOSFETs, modeled in COMSOL Multiphysics. Comparisons between similar AlGaN/GaN and Si power modules are made in terms of heatsink requirements. The temperatures for the same operating voltage are found to be significantly lower for the AlGaN MOSFETs structures, compared to Si. The heatsink size for the AlGaN/GaN is found to be smaller compared to Si for the power modules.
Heave Compensation Dynamics for Offshore Drilling Operation
Authors: Kim Dave , Ku Namkug
Published: September 2021

In this study, dynamic response analysis of a heave compensation system for offshore drilling operations was conducted based on multibody dynamics. The efficiency of the heave compensation system was computed using simulation techniques and virtually confirmed before being applied to drilling operations. The heave compensation system was installed on a semi-submersible and comprises several interconnected bodies with various joints. Therefore, a dynamics kernel based on multibody dynamics was developed to perform dynamic response analysis. The recursive Newton–Euler formulation was adopted to construct the equations of motion for the multibody system. Functions of the developed dynamics kernel were verified by comparing them with those from other studies. Hydrostatic force, linearized hydrodynamic force, and pneumatic and hydraulic control forces were considered the external forces acting on the platform of the semi-submersible rig and the heave compensation system. The dynamic simulation was performed for the heave compensation system of the semi-submersible rig for drilling operations up to 3600 m water depth. From the results of the simulation, the efficiency of the heave compensation system was evaluated to be approximately 96.7%.
Biological and Ecological Aspects of the Blackmouth Catshark (Galeus melastomus Rafinesque, 1810) in the Southern Tyrrhenian Sea
Authors: D’Iglio Claudio , Albano Marco , Tiralongo Francesco , Famulari Sergio , Rinelli Paola , Savoca Serena , Spanò Nunziacarla , Capillo Gioele
Published: September 2021

Data on the biology and ecology of Galeus melastomus are old/absent for the Southern Tyrrhenian Sea, despite there being numerous studies in the wider area. A total of 127 specimens of G. melastomus from the southern Tyrrhenian Sea, collected in 2018–2019 using trawling nets, were analyzed to investigate size at sexual maturity, sex ratio, length–weight relationships, and feeding habits. To our best knowledge, this is the first time in which all these features were investigated in the Southern Tyrrhenian Sea for G. melastomus. The stomach content analysis showed that G. melastomus had intermediate feeding habits, preying on a great variety of species, especially Cephalopoda, Osteichthyes, and Crustacea. The Levin’s index value (Bi) was 0.53. Sex ratio was 0.92:1, with females slightly more abundant and bigger than males. The results also showed a decrease (33.7 cm for females, 31.1 cm for males) in length at 50% maturity (L50). This could be a result of anthropogenic stressors, such as overfishing and/or and environmental changes, which can induce physiological responses in several species. Our results highlighted the differences related to sexual maturity, growth, and feeding habits of the blackmouth catshark in the studied area, providing reference data to allow comparison with future studies on this species adaptations to this and other deep-sea areas in the Mediterranean Sea.
First-Year Survival of Northern Fur Seals (Callorhinus ursinus) Can Be Explained by Pollock (Gadus chalcogrammus) Catches in the Eastern Bering Sea
Authors: Short Jeffrey W. , Geiger Harold J. , Fritz Lowell W. , Warrenchuk Jonathan J.
Published: September 2021

The Pribilof northern fur seal (Callorhinus ursinus) herd in the eastern Bering Sea has declined by ~70% since the 1970s, for elusive reasons. Competition for pollock (Gadus chalcogramma) with the commercial fishery has been suspected as a contributing factor, but no correlative relationship between fishing activity and fur seal population declines has heretofore been demonstrated. Here, we present evidence for a moderately strong inverse relationship between fishery catches of pollock and first-year survival of fur seals, based on three different approaches to evaluation. We suspect this relationship results from the dependence of lactating female fur seals on locating dense and extensive schools of pollock near the Pribilof Islands to efficiently provide nutrition for their pups, because the pollock fishery also targets these same schools, and when fished, the remnants of these schools are fragmented and dispersed, making them more difficult for fur seals to locate and exploit. Inadequately fed pups are less likely to survive their initial independent residence at sea as they migrate south from the Pribilof Islands in the fall. Our results imply that pollock catches above ~1,000,000 t within ~300 km of the Pribilof Islands may continue to suppress first-year survival of Pribilof fur seals below the estimated equilibrium survival value of 0.50, leading to continued decline of the population.
Prediction of Wave Energy Transformation Capability in Isolated Islands by Using the Monte Carlo Method
Authors: Avila Deivis , Marichal Graciliano Nicolás , Quiza Ramón , San Luis Felipe
Published: September 2021

In this work, a mathematical computer simulation model is used to predict the possible energy generated from different Waves Energy Converters (WECs) in the Canary Islands. The Monte Carlo Method is the computer simulation model proposed to predict the generated energy. The Waves Energy Converter systems analyzed in the study were, the Aqua Buoy, Wave Dragon and Pelamis converters. The models were implemented and validated, with the dataset of Gran Canaria deep water buoy. This buoy belongs to a network of buoys belonging to Spain’s State Ports and they cover a dataset period of 22 years. The research has concluded that it is possible to affirm that the achieved model is a strong tool to compute the possible energy of any WECs, when the power matrix is known. The model based on the Monte Carlo simulation can be used in isolated islands of the Atlantic Ocean and can be extrapolated to other regions with the same characteristics.
Do We Need Trust Transfer Mechanisms? An M-Commerce Adoption Perspective
Authors: Sim Jia Jia , Loh Siu Hong , Wong Kee Luen , Choong Chee Keong
Published: September 2021

This study aimed to provide a comprehensive analysis of the factors that determine and shape consumers’ behavioral intention to adopt mobile commerce (m-commerce). By integrating the core constructs from the Unified Theory of Acceptance and Use of Technology (UTAUT), together with the trust-building mechanisms, this study explored the importance of the institutional mechanisms and their moderating effects between trust in the vendors and intention to adopt m-commerce. Traditionally, the effects of institutional mechanisms on trust and adoption intention have been considered separately in different study contexts. The purpose of this study was to extend the literature by simultaneously exploring two institutional mechanisms that are conceptually highly similar to each other, namely, structural assurance (SA) and perceived effectiveness of e-commerce institutional mechanisms (PEEIM). A self-administered survey was used to collect data, which were analyzed using partial least squares structural equation modelling (PLS-SEM). The results revealed that most of the constructs examined have significant relationships with the intention to adopt m-commerce. Additionally, PEEIM exhibits a significant moderating effect but SA does not. This study delineates how trust-building mechanisms play important roles in increasing consumers’ confidence in order to promote m-commerce adoption.
Land Use Transitions under Rapid Urbanization: A Perspective from Developing China
Authors: Long Hualou , Kong Xiangbin , Hu Shougeng , Li Yurui
Published: September 2021

Land use transition is a manifestation of land use and land cover change (LUCC) and is also a major research focus of the Global Land Project (GLP), as well as land system science (LSS) [...]