bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrating Pluralism and Multicultural Education to Prevent Radicalism at Universitas Muhammadiyah Kupang
Authors: Syahrul Syahrul
Published: November 2021

The study aimed to reveal how to integrate pluralism and multicultural education to prevent radicalism at Universitas Muhammadiyah Kupang. This qualitative research used purposive sampling technique to select the informants between students and lecturers. The data collections were analysed by using the Creswell method, such as data collection, organization, reading, memo, description, classification, interpretation, and visualization. The study showed that integrating pluralism and multicultural education was conducted through selecting content integration, using cooperative learning method, constructing knowledge, reducing prejudices, and evaluating the learning process. As a result, the outcome of the learning process shows that radicalism can be prevented through integrating pluralism and multicultural education into curriculum of sociology education.
Model pembelajaran two stay two stray, disiplin belajar dan kesiapan belajar : pengaruh terhadap motivasi belajar Ekonomi siswa kelas XI IPS
Authors: Meliana Ika , Hariani Lilik Sri , Afian Affan
Published: November 2021

Motivasi Belajar Ekonomi merupakan salah satu aspek yang dapat mendorong siswa untuk tertarik pada mata pelajaran Ekonomi, ada beberapa faktor yang dapat mempengaruhi Motivasi Belajar Ekonomi diantaranya; Model Pembelajaran Two Stay Two Stray, Disiplin Belajar dan Kesiapan Belajar. Penelitian ini bertujuan untuk menganalisis (1) Pengaruh Model Pembelajaran Two Stay Two Stray, Disiplin Belajar dan Kesiapan Belajar terhadap Motivasi Belajar Ekonomi Siswa kelas XI IPS SMAN 5 Malang. (2) Pengaruh Model Pembelajaran Two Stay Two Stray terhadap Motivasi Belajar Ekonomi Siswa. (3) Pengaruh Disiplin Belajar terhadap Motivasi Belajar Ekonomi Siswa. (4) Pengaruh Kesiapan Belajar Terhadap Motivasi Belajar Ekonomi Siswa. Penelitian ini menggunakan pendekatan kuantitatif, jenis penelitian ini adalah penelitian kuantitatif. Metode statistik penelitian ini menggunakan regresi linear berganda. Populasi dalam penelitian ini adalah siswa kelas XI IPS SMAN 5 Malang sebesar 78 siswa, tehnik pengambilan sampel penelitian menggunakan sampel jenuh sejumlah 78 siswa. Tehnik analisis data dalam penelitian ini menggunakan analisis regeresi linear berganda, untuk uji prasyarat/asumsi klasik terbagi menjadi 4 yaitu uji normalitas, uji multikoliniaritas, uji heterokedastisitas, uji autokorelasi. Hasil pada penelitian ini berdasarkan uji hipotesis menggunakan analisis regeresi linear berganda secara simultan terdapat pengaruh yang signifikan antara variabel bebas terhadap variabel terikat. Secara parsial model pembelajaran Two Stay Two Stray berpengaruh signifikan terhadap Motivasi Belajar Ekonomi, Disiplin Belajar berpengaruh signifikan terhadap Motivasi Belajar Ekonomi, Kesiapan Belajar berpengaruh signifikan terhadap Motivasi Belajar Ekonomi.
Pembentukan kemandirian melalui pembelajaran kewirausahaan sosial untuk meningkatkan kesadaran sosial dan kesadaran ekonomi
Authors: Andayani Endah , Hariani Lilik Sri , Jauhari Muchammad
Published: November 2021

The establishment of self-reliance through social entrepreneurship learning to increase students' socioeconomic awareness is an important issue, especially in formal educational institutions that are vulnerable from global influence, in the direction of policy and strategic direction of the Directorate of Vocational Education development focuses on motivating schools to progress, regrow discipline and responsibility, mental development and noble morals of vocational school students, and the creation of a resilient and independent young generation. This study aims to describe in detail how the establishment of self-reliance through social entrepreneurship activities can increase the socioeconomic awareness of the students and why this should be done. In accordance with the characteristics of vocational school students, who are required to have work-ready competencies and even able to create a world of work for others so as to provide socioeconomic changes, as well as re-encourage social entrepreneurship activities to help create self-reliance on socioeconomic activities faced in society. This research uses qualitative approach with retrospective case study model at SMK Islam Terpadu Asy-Ayadzili Pakis Malang Regency and conducted during the ongoing incident. This research method is intended to obtain a model or social engineering about the process of establishing proper independence through social entrepreneurship and the reason for the need for self-reliance. The results found that the establishment of self-reliance through social entrepreneurship to improve social and economic welfare is a very strong reason implemented social entrepreneurship. Constraints in developing entrepreneurial values in students are low student confidence, limited time sharing with pesantren activities, strict rules and schedules in the cottage, lack of cooperation between students, difficulty finding the right business.
Efikasi diri, pendidikan kewirausahaan dan ekspektasi pendapatan : pengaruh terhadap minat berwirausaha
Authors: Satyantoro Ilham Pragosa , Andayani Endah , Walipah
Published: November 2021

ABSTRAK: Penelitian ini membahas tentang Pengaruh Efikasi Diri, Pendidikan Kewirausahaan dan Ekspektasi Pendapatan terhadap Minat Berwirausaha. Populasi dalam penelitian ini adalah mahasiswa Pendidikan Ekonomi Universitas Kanjuruhan Malang sejumlah 55 mahasiswa, Teknik pengambilan sample menggunakan sampel jenuh sejumlah 55 mahasiswa. Jenis penelitian ini adalah penelitian kuantitatif. Metode statisik penelitian ini menggunakan Regresi Linier Berganda dengan prasyarat uji asumsi klasik uji normalitas, uji multikoloniaritas, uji heteroskesdastisitas, uji autokorelasi pada pengujian hipotesis menggunakan uji F, uji t, uji R. Berdasarkan uji hipotesis menggunakan analisis Regresi Linier Berganda, pada penelitian ini terdapat pengaruh yang signifikan antara variabel bebas terhadap variabel terikat. variabel X1 (Efikasi Diri) berpengaruh signifikan terhadap variabel Y(Minat berwirausaha) dengan nilai signifikan sebesar 0,004, X2(Pendidikan Kewirausahaan) berpengaruh signifikan terhadap Y(Minat Berwirausaha) dengan nilai signifikan sebesar 0047, X3 (Ekspektasi Pendapatan) berpengaruh terhadap variabel Y(Minat Berwirausaha) dengan nilai signifikan sebesar 0,43.
Digitalisasi sebagai upaya adaptif industri pasar modal di era disruption: dampak pada eksistensi WPPE di masa pandemi Covid-19
Authors: Murdiyanto Edi , Akbar Taufik , Sari Ati Retna
Published: November 2021

This research is based on the number of securities companies that have begun to focus on the phenomenon of their business on online services, both online account opening and online trading media transactions as an effort to adapt to the current disruption. The research was conducted with a qualitative approach, by triangulating research data. Not all data were obtained in full due to limited access obtained by researchers. From the results of the study, there was an increase in the number of investors in the capital market during the COVID-19 pandemic. On the other hand, there is a decrease in the number of securities companies that are members of the stock exchange, as well as the WPPE licensed brokerage profession which is facing serious challenges due to this phenomenon.
Passenger satisfaction and decision of repurchasing on rail transportation services
Authors: Arianti Venny , Pahala Yosi , Setyawati Aswanti , Ricardianto Prasadja
Published: November 2021

The purpose of the study was to determine and analyze the effect of train rates, comfort and timeliness on passenger satisfaction and the impact of repurchase decision of train transportation services for the Jenggala Train on the Sidoarjo-Mojokerto route, at East Java, Indonesia. The main problem, there are complaints about unsatisfactory service, inaccuracy of departure and arrival time at the station aim. Also, the passenger capacity provided is large, but the volume transport is still minimal and there is no data on passengers who make repeat purchases. The sample are 100 Jenggala train crossing service Sidoarjo-Mojokerto passengers. Method of data analyze used path analysis. The result showed that the changes of rate amount, experience of passenger comfort, and timeliness of train travel had major impact on the decision of repurchase. As a theorithical and practical implication, the improvement of service user facilities at the station and on the train is very important needed to create comfort for users of transportation services train, because the comfort variable has a significant effect dominant in influencing purchasing decisions.
Dampak pengumuman Covid-19 terhadap harga dan volume saham pada industri farmasi di Indonesia
Authors: Gunarso Pujo , Nathaniel Jason , Dewi Ani Rustia
Published: November 2021

The purpose of this study is to analyze the effect of the announcement of Covid-19 in Indonesia on stock prices and the volume of shares traded by the pharmaceutical industry listed on the Indonesia Stock Exchange in 2020. Research on the pharmaceutical industry with a sample of 10 companies. The research period is 360 days or 180 days before and after the announcement of Covid-19. The event study methodology analyzes the market reaction to an event, namely the announcement of the Covid-19 pandemic and data analysis techniques using the Wilcoxon Sign Rank Test. The results of the study explain that there are insignificant differences in share prices and significant differences in the volume of shares traded, which means that the announcement of Covid-19 has information content as indicated by an increase in the volume of shares traded.
STATISTICAL LITERACY OF SECONDARY SCHOOL STUDENTS IN SOLVING CONTEXTUAL PROBLEMS TAKING INTO ACCOUNT THE INITIAL STATISTICAL ABILITY
Authors: Risqi Erlyanna Nur , Rini Setianingsih
Published: November 2021

Statistical literacy is strongly needed in the 21st century in reading data information, thinking critically and making decisions. The purpose of this study is to describe the statistical literacy abilities of high school students in solving contextual problems taking into account their initial statistical abilities, with the following indicators, Tier 1 (reading & explaining data), Tier 2 (analyzing, interpreting, interpreting critically, and making conclusions), Tier 3 (presenting data and predicting something). This research is a qualitative in nature, using statistical ability test, statistical literacy test, and interview guidelines to collect data. The subjects of this study consist of three female students of grade XII Senior High School, with high, medium and low initial statistical ability categories. The results of this study revealed that (1) The student with high initial statistical abilities can meet the indicators of Tier 1, Tier 2, Tier 3, so that she can be categorized at level 5 to 6 (critical-mathematics critical), (2) The student with medium ability can fulfil the Tier 1 indicator not fulfil the indicators in Tier 2 and Tier 3, so that it can be categorized at level 3 to 5 (Consistent noncritical-Critical), and (3) The students with low abilities can fulfill the indicators of Tier 1 not fulfil the indicators in Tier 2 and Tier 3, so that it can be categorized at level 1 to 3 (Idiosyncratic-Consistent Noncritical). Therefore, it is important for students and teachers to know statistical literacy skills in order to increase their understanding and ability to understand information in making decisions regarding daily problems.
Evaluation of the Success Rate of Emergency Cerclage by Double- Suture Method in Pregnant Women With the Prolapsed Amniotic Membrane
Authors: Emami Ali , Dadashaliha Masumeh
Published: November 2021

BackgroundCervical insufficiency is a risk factor for preterm labor and second trimester loss. Cervical cerclage is the choice treatment for women with this risk factor. An emergency cerclage is recommended in cases with the prolapsed amniotic membrane at the dilated cervical os before 24 weeks of gestation. The aim of this study is to report our method with double-suture in emergency cerclage. MethodsThis is a cross-sectional study conducted between March 2017 and February 2019 of women who received the double-suture cerclage in Qazvin referral Kosar hospital. The inclusion criteria were women with gestational age of 18-24 weeks with the prolapsed amniotic membrane and cervical dilatation (more than 3-4cm) in the speculum exam. The exclusion criteria were the mother's dissatisfaction to do this cerclage, previous failure cerclage, twin pregnancy, preterm rupture of membrane (PROM) signs and chorioamnionitis signs.ResultsFive women were included. All of them presented prolapsed membranes at or beyond the cervical os. The double-suture cerclage was performed for all of the patients. All of women delivered by normal vaginal delivery and their infants had no problems. ConclusionsDespite the small number of the patients, this study showed that this method of cerclage was effective in pregnancy for women with high risk of preterm labor and second trimester loss. This technique requires an expert and trained surgical team. The following studies should be performed with a big population and compare with other methods of emergency cerclage.
Association Between the Diabetes Drug Cost and Cardiovascular Events for Type 2 Diabetes in Korea: A National Health Insurance Service Database Analysis
Authors: Chung Seung Min , Lee Ji-In , Han Eugene , Seo Hyun-Ae , Jeon Eonju , Kim Hye Soon , Yoon JiSung
Published: November 2021

Background: We aimed to investigate the long-term effects of diabetes drug costs on cardiovascular (CV) events and death. Methods: This retrospective observational study used the 2009–2018 National Health Insurance data in Korea. Among patients with type 2 diabetes, those who were taking antidiabetic drugs and did not have CV events before 2009 were included. Data on the annual cost of each diabetes drug were collected. The 10-year incidence of CV events (cardiac death, non-fatal myocardial infarction, stroke, hospitalization for heart failure, and coronary revascularization) and CV death were analyzed according to the diabetes drug cost quartiles (Q1 [the lowest] – Q4 [the highest]).Results: A total of 441,914 participants were enrolled (median age, 60 years; male, 57%). CV events and death occurred in 28.1% and 8.36% of patients, respectively. The overall diabetes drug cost was USD 271/year per person (range: 1–18,921). The 10-year incidence of CV events and death was lowest in Q3 and high in Q4 and Q1. After adjusting for CV risk factors, the risk of CV events increased in the sequential order of cost quartiles (hazard ratio (HR)=1 [ref], 1.102 [95% confidence interval (CI): 1.084–1.120], 1.137 [95% CI: 1.118–1.156], and 1.323 [95% CI: 1.3011.346]). The risk of CV death showed U-shaped pattern which was lowest in the Q3 (HR=0.943, 95% CI 0.903-0.984) and highest in the Q4 (HR= 1.234, 95% CI 1.182-1.287).Conclusion: The expenditure for diabetes drug affects 10-year CV events and death. Affording an appropriate diabetes cost at a similar CV risk is an independent protective factor for CV death.Trial registration: retrospectively registered
QT Interval Instability and Variability in Dogs with Hyperadrenocorticism
Authors: Vila Beatriz de Carvalho Pato , Vanhoni Marcela Sigolo , Sousa Marlos Gonçalves
Published: November 2021

Hyperadrenocorticism is one of the most common endocrine diseases in dogs. In humans, it is clearly associated with a higher risk of cardiovascular events, but studies in dogs are scarce. To investigate the arrhythmogenic risk of dogs with hyperadrenocorticism, indices of variability and instability of the QT interval were studied in 38 dogs with hyperadrenocorticism and in 12 healthy dogs: variance (QTv), total instability (TI), short-term (STI) and long-term (LTI), and mean (QTm). Except for QTm, all parameters studied were higher in the hyperadrenocorticism group than in the control group. In addition, STI and QTv showed moderate positive correlation with left ventricle wall thickness. To a better understanding on the effect of the hypothalamus-pituitary-adrenal axis on ventricular repolarization, the hyperadrenocorticism group was subdivided according to the percentage of variation in plasma cortisol concentration (<30.1%; 30.1-60%; >60%) 8 hours after low-dose administration of dexamethasone. There was statistical difference in QTv, TI and LTI indices between the control group and the <30.1% and >60% groups, and in STI index between the control group and the >60% group. There was no statistical difference between sex groups in any of the electrocardiographic parameters studied. This result may indicate that the etiology of hyperadrenocorticism, and its consequent influence on hypothalamus-pituitary-adrenal axis could interfere on the heterogeneity of ventricular repolarization parameters in different ways, especially in the short-term stability; however further studies are necessary to understand the role of cortisol on electrical instability in dogs.
Relationship Between Emotion Regulation and Non-suicidal Self-injury (NSSI): a Systematic Review and Meta-analysis Protocol
Authors: Taherifar Zahra , Mousavi Nasim , AghaMohammadi Soudeh , Zeinodini Zahra , Rostami Hanieh Goli , Farahani Hanieh Sh. , Norozpour Marzieh , Keshtkar Abbas
Published: November 2021

Background: Non-suicidal self-injury (NSSI) is recognized a serious mental disorder. Its prevalence has increased in recent years. Emotion regulation has been noted to have important role in mental health problems. There is evidence in the literature about the relationship between NSSI and emotion regulation. The studies, however, have reported mixed findings, and no comprehensive study has been conducted yet to address this topic. Therefore, the aim of this systematic review and meta-analysis is to investigate the relationship between NSSI and emotion regulation.Methods: All studies published after 1990 that have assessed the relationship between NSSI and emotion regulation will be eligible. This entails observational-analytic (descriptive or cross-sectional) studies, case-control studies, cohort studies. There will be no language restriction. Additionally, no limitation will be imposed regarding age, gender, and nationality of the subjects studiedDiscussion: Given the inconsistencies present in the findings of primary studies in terms of relationship between NSSI and emotion regulation strategies conducting such a study seems necessary. The results of this study could be used to improve our knowledge about NSSI, help revise health policies in the future, research and education to reduce NSSI burden, and to develop more effective interventions to manage NSSI.Systematic review registration: CRD42021226454
Risk Factors Associated with Dysphagia after Anterior Surgery in Treatment for Multilevel Cervical Disorder with Kyphosis
Authors: li yongjun , wei xing , Chen Bingyao , wang feng , Song Guangze , shen yong
Published: November 2021

Study Design: A retrospective study.Objective: The purpose of this study was to clarify the risk factors associated with dysphagia following anterior surgery treating multilevel cervical disorder with kyphosis based on subgroup of follow-up time.Methods: Totally, 81 patients who suffered from multilevel cervical disorder with kyphosis receiving anterior surgery from July 2018 to June 2020 were reviewed for clinical and radiological outcomes. Patients with dysphagia was defined as dysphagia group and without dysphagia as no-dysphagia group according to follow-up time (1 week, 1-month, 3-month, 6-month and 1-year after surgery). Data was performed to compare between the patients with and without dysphagia.Results: In our study, the occurrence of dysphagia was 67.9%, 44.4%, 34.6%，25.9% and 14.8% at the time of 1 week, 1-month, 3-month, 6-month, and 1 -year after surgery, respectively. Our findings showed that a history of smoking, lower preoperative Swallowing Quality of Life (SWAL-QOL) score, post-operative Cobb angle of C2-7 and change of Cobb angle of C2-7 were associated with dysphagia within 3-month after surgery. Furthermore, a history of smoking, lower preoperative SWAL-QOL score, and post-operative Cobb angle of C2-7 were linked with dysphagia within 6-month after surgery. However, a history of smoking and lower preoperative SWAL-QOL score were found to be risk factors related with dysphagia at at any follow-up.Conclusions: In the present study, many factors were related with dysphagia during 3-month after surgery. What’s more, a history of smoking and lower preoperative SWAL-QOL score were associated with dysphagia at any follow-up. We hope this article can provide a reference for spinal surgeons to predict which patients were susceptible to suffer from dysphagia after anterior surgery in treatment of multilevel cervical disorder with kyphosis.
Matrix Completion of World Trade
Authors: Gnecco Giorgio , Nutarelli Federico , Riccaboni Massimo
Published: November 2021

This work applies Matrix Completion (MC) – a class of machine-learning methods commonly used in the context of recommendation systems – to analyze economic complexity. MC is applied to reconstruct the Revealed Comparative Advantage (RCA) matrix, whose elements express the relative advantage of countries in given classes of products, as evidenced by yearly trade flows. A high-accuracy binary classifier is derived from the MC application, with the aim of discriminating between elements of the RCA matrix that are, respectively, higher/lower than one. We introduce a novel Matrix cOmpletion iNdex of Economic complexitY (MONEY) based on MC, and related to the degree of predictability of the RCA entries of different countries (the lower the predictability, the higher the complexity). Differently from previously-developed economic complexity indices, MONEY takes into account several singular vectors of the matrix reconstructed by MC, whereas other indices are based only on one/two eigenvectors of a suitable symmetric matrix, derived from the RCA matrix. Finally, MC is compared with a state-of-the-art economic complexity index (GENEPY), showing that the false positive rate per country of a binary classifier constructed starting from the average entry-wise output of MC is a proxy of GENEPY.
Microbiome Analysis Exploring Taxonomic Diversity in Kasaragod Dwarf and Holstein Crossbred Cattle
Authors: M Deepthi , Arvind Kumar , Saxena Rituja , Pulikkan Joby , Sharma Vineet K , GRACE TONY
Published: November 2021

The indigenous cattle are efficient in converting low quality feeds and forage into animal products. Kasaragod Dwarf cattle, a unique non-descriptive native cattle of Kerala, India, are noted for their unique qualities, such as low feed intake, thermotolerance, greater resistance to diseases and A2 allelic variant milk. However, owing to the higher milk yield, Holstein crossbred cattle are given more importance over Kasaragod Dwarf. The hindgut microbiota plays a major role in various biological processes such as the digestion, vitamins synthesis, and immunity in cattle. In this study, we compared the hindgut microbiota of the Kasaragod Dwarf with the highly found, Holstein crossbred utilizing 16S rRNA high-throughput sequencing for a better understanding of the relationship between the host and microbial community. Four replicates of each 20 samples comprising two cattle type (n=10) were sequenced and analyzed. Marker gene-based taxonomic analysis affirmed variations in their microbial composition. Principle Coordinate Analysis (PCoA) using weighted and unweighted UniFrac distance matrices showed the distinct microbial architecture of the two cattle type. Random Forest analysis further confirmed the distinctness and revealed the signature taxa in K-Dwarf. The study observed the predominance of feed efficiency associated genera viz., Anaerovibrio, Succinivibrio, Roseburia, Coprococcus, Anaerostipes, Paludibacter, Elusimicrobium, Sutterella, Oribacterium, Coprobacillus, and Ruminobacter in Kasaragod Dwarf cattle. The study highlights the abundance of unique and beneficial hindgut microflora found in Kasaragod Dwarf, which may attest its importance over exotic cattle breeds viz., Holstein. To our knowledge, this is the first report of Kasaragod Dwarf cattle gut microbiome profiling. This study is pivotal towards developing genetic resources for the microbial population in K-Dwarf and how it could be differentiated from Holstein crossbred cattle.
Circ-0010928 Negatively Regulates Hypoxia-Induced Endothelial Cell Angiogenesis Through The miR-921/LSM14A axis.
Authors: Zhang Tian Rong , Huang WeiQiang
Published: November 2021

Background

Angiogenesis is an important factor in promoting vascular repair and a valuable process in the treatment of cardiovascular diseases. Circular RNAs (circRNAs) are widely expressed in eukaryotic cells and play an important role in the regulation of endothelial cells (ECs). In our study, bioinformatics analysis and real-time fluorescent PCR detection revealed that circRNA 0010928 (circ-0010928) is differentially expressed in human cardiac microvascular endothelial cells (HCMECs).
Material & Methods

We evaluated the role of circ-0010928 in HCMECs. Then, we can verify the function of circ-0010928 in HCMECs by cell counting kit-8 (CCK8), scratch test, transwell experiment, tube forming experiment, flow cytometry. Use dual luciferase experiment to detect the binding relationship between circ-0010928, miR-921 and LSM14A.
Results

Overexpression of circ-0010928 inhibited the proliferation, migration and tube formation of HCMECs under hypoxic conditions and promoted their apoptosis. In addition, dual luciferase reporter assays confirmed that circ-0010928 acted as a sponge of miR-921 and LSM14A as a downstream target gene of miR-921. Silencing miR-921 could also inhibit the proliferation, migration and tube formation of HCMECs and negatively regulate angiogenesis.
Conclusion

CircRNA-0010928 may inhibit the function of miRNA-921by combining with miRNA-921, and then miRNA-921 plays a role in regulating LSM14A, thereby regulating the state of angiogenesis.
Activation of The P62-Keap1-NRF2 Pathway Protects Against Ferroptosis in Radiation-Induced Lung Injury
Authors: Li Xuan , Chen Jingyao , Yuan Sujuan , Zhuang Xibing , Qiao Tiankui
Published: November 2021

Background

Radiation-induced lung injury (RILI) is one of the most common, serious and dose-limiting complications of thoracic radiotherapy. A primary reason for this is the radiation-induced cell death. Ferroptosis is a recently recognized form of regulated cell death, characterized by the accumulation of lipid peroxidation products and lethal reactive oxygen species (ROS). The ROS induced by irradiation might be the original trigger of ferroptosis in RILI. Furthermore, activation of the P62-Kelch-like ECH-associated protein 1 (Keap1)-nuclear factor erythroid 2-related factor 2 (NRF2) pathway has been shown to exert a protective effect, blunting ferroptosis. Therefore, this study aims to explore the protective effect of the P62-Keap1-NRF2 pathway against radiation-induced ferroptosis in alveolar epithelial cells.
Results

Firstly, our results demonstrated that radiation induced ferroptosis in vitro RILI cell model, which could be significantly reduced by Ferrostatin-1 (Fer-1), a specific inhibitor of ferroptosis. Then, we found that overexpression of P62 interacted with Keap1 to promote NRF2 translocation into the nucleus and upregulation its target proteins quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HO1) and ferritin heavy chain 1 (FTH1).
Conclusion

Collectively, the activation of the P62-Keap1-NRF2 pathway prevents radiation-induced ferroptosis in RILI cells, providing a theoretical basis for further research to find a potential approach for RILI therapy.
Rhizo-inoculation of phosphate solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions.
Authors: Ibrahim Musa Saheed , Ikhajiagbe Beckley
Published: November 2021

Background
The research investigated the possibility of phosphate solubilizing bacteria (PSB) with plant growth-promoting (PGP) capabilities to improve growth properties of rice plant under ferruginous ultisol (FU) condition through rhizo-inoculation strategy. The PSB with PGP properties used in this research were Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 that were previously isolated and characterized following the 16S rRNA gene sequencing. The rice seeds were sown in a composite FU soil sample and a humus soil (control) and then rhizo-inoculated along the root region of the growing rice seedling at 16 days after sowing. The rice plant was studied for differences in morphological, physiological and biomass parameters for 16 weeks after rhizo-inoculation.
Results
The composite FU soil used in the study had high pH, low bioavailable phosphorus, low water holding capacity and high iron levels which has led to a low growth properties of rice seeds sown in FU soil without rhizo-inoculation. After rhizo-inoculation, a significant increase in morphological, biomass and physiological parameters were observed in the rice plant grown in the FU soil as against the control and the rice plant in FU soil without inoculation except for terpenoid which is usually known to signify biotic stress and as part of plant defense mechanism. Generally, Bacillus cereus strain GGBSU-1 showed higher improvement compared to other PSBs used in the present study. This is due to a more improved growth properties observed.
Conclusion
Rhizo-inoculation of rice seedling with Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 under FU soil condition significantly improved growth properties of the rice plant. This suggest the ability of PSB to solubilize and mineralize soil phosphate and improve its availability for plant use in phosphate stressed soil, thereby improving plant growth properties.
The Effects of Vegetation Restoration and Season On Soil Enzyme Activity and Microbial Communities in Karst Rocky Desertification Area
Authors: Guo Zhang , Zheng Chunyan , Chen Zhu , Wang Jin , Yu Yanghua , Liu Ziqi , Yang Guangbin , Xiong Kangning
Published: November 2021

Aims The process of karst rocky desertification has been closely related to improper land use in southwest China. Now this habitat is the subject of an important ecological restoration project. However, the changes in soil properties and microbial characteristics in response to this vegetation restoration remain poorly understood.Methods We investigated four vegetation types, including dragon fruit, Chinese pepper, walnut teak, with corn as a control, in southwest China, in 2019. We measured the impacts of these vegetation types on soil properties and microbial biomass, enzyme activity, and microbial community composition (using high-throughput sequencing technology).Results The different vegetation types had significantly different impacts on soil exchangeable Ca2+, soil organic carbon and available nutrients. The vegetation types also significantly affected microbial biomass. Soil enzyme activity, including b-1,4-glucosidase, b-1,4-N-acetylglucosaminidase, alkaline phosphatase, and catalase, were significantly different among vegetation types. All vegetation types were dominated by the bacterial phyla Acidobacteria, Proteobacteria, and Actinobacteria and the fungal phylum Ascomycota, except for corn which was dominated by the fungal phylum Mucoromycota. Non-metric multidimensional scaling (NMDS) showed that the vegetation type exhibited different microbial b-diversity, especially in winter. The vegetation type, season, and soil properties collectively explained 46% and 59% of soil bacterial and fungal community composition, respectively. The bacterial-fungal interactions under the six vegetation types were distinctly different between summer and winter.Conclusions Compared with traditional corn, the restoration of natural vegetation partially reversed KRD by improving soil properties, increasing microbial biomass, and differentiating the microbial community structures in the different vegetation types.
Nitrogen Application Increases Soil Organic Carbon And Crop Productivity By Altering Soil Autotrophic Bacterial Community On the Semiarid Loess Plateau
Authors: Wang Jinbin , Xie Junhong , Li Lingling , Luo Zhuzhu , Zhang Renzhi , Jiang Yuji
Published: November 2021

Background and aims Soil autotrophic microorganisms play crucial roles in carbon fixation and crop productivity. However, the information is remains limited to whether and how soil autotrophic microbe mediate SOC pool dynamics and crop productivity.Methods Here, we investigated the effects of the structure and co-occurrence network of soil autotrophic bacterial community on SOC storage and maize yield. A long-term field experiment was conducted with four chemical nitrogen (N) application rates on the semi-arid Loess Plateau, including N application at 0 kg ha–1 (N0), 100 kg ha−1 (N1), 200 kg ha−1 (N2), and 300 kg ha−1 (N3), respectively. Results Our results showed that the N application significantly impacted the structure and co-occurrence network of soil cbbL-carrying bacterial community via changing soil pH, nitrate nitrogen (NO3-N), and soil water content (SWC). The diversity of soil autotrophic bacterial community decreased with the increasing rate of N application. We detected a high abundance of the autotrophic bacterial dominant genera Xanthobacter, Bradyrhizobium, Aminobacter, and Nitrosospira. The co-occurrence network of autotrophic bacteria contained four distinct modules that consisted of closely interconnected microorganisms. Structural equation modeling further indicated that the diversity, composition, and network of autotrophic bacterial community had significant relationships with SOC storage and maize yield.Conclusion Taken together, our results highlight that the soil autotrophic bacterial community may drive carbon fixation SOC accumulation and contribute positively to crop productivity.
The correlation of fibrinogen-like protein-1 expression with the progression and prognosis of hepatocellular carcinoma
Authors: Hua Nanni , Yang Chen , Chen Anxian , Feng Yi , He Xianglei , Ru Guoqing , Tong Xiangmin , Wang Shibing
Published: November 2021

Background

ConclusionsFibrinogen-like protein-1 (FGL1), as the member of FREP superfamily, had been widely concerned as a major immune inhibitory ligand of LAG-3. Although FGL1 expression levels didn’t have significant difference in most of tumors via online data analysis, we found that it was down-regulated in liver cancer. Moreover, the correlation between FGL1 expression and the progression and prognosis of hepatocellular carcinoma (HCC) was still disputed.
Methods

In our study, firstly, we used bioinformatics analysis to define the expression profile and clinical significance of FGL1 in HCC. Then, we determined the FGL1 level in human HCC cell lines, tumor and normal liver tissues from HCC patients by western blot. Furthermore, tissue microassays were used to detect the expression of FGL1 through immunohistochemistry staining and verify whether FGL1 expression levels were associated with clinicopathological features of HCC patients.
Results

The results proved that FGL1 was down-regulated significantly in HCC cell lines and HCC tissues, corresponding with the results of our bioinformatics analysis. FGL1 expression levels in HCC were related to Edmondson grade and metastasis. Additionally, high FGL1 expression was related to better overall survival in HCC patients, indicating that the down-regulated FGL1 was correlated with poor prognosis and FGL1 might function as a tumor suppressor.
Conclusions

Taken together, expression levels of FGL1 may correlate with the progression and prognosis of HCC, and FGL1 could be a potential prognostic biomarker.
Predictive Value of the PaO2/FIO2 Ratio for Mortality in Patients with Acute Respiratory Distress Syndrome: A Systematic Review and Meta-Analysis
Authors: Yoshimura Satoshi , Hashimoto Katsuhiko , Shono Yuji , Tamura Takahiro , Uchimido Ryo , Ando Koichi , Okamori Satoshi , Yoshida Takuo , Yoshitake Shigenori , Okada Yohei
Published: November 2021

BackgroundPaO2/FIO2 (P/F) ratio has been used to define the severity of acute respiratory distress syndrome (ARDS) despite the controversy of its clinical utility. This systematic review and meta-analysis (SRMA) aimed to obtain summary estimates of predictive performance of the P/F ratio for predicting mortality in ARDS patients.MethodsWe included a study wherein the study population comprised ARDS patients in anyclinical setting. Medline and Cochrane Central Registry of Controlled Trials were searched for all English language articles. We performed a SRMA on the accuracy of diagnostic prognostic tests using QUADAS-2 tool to evaluate the risk of bias. To pool the results, we applied the bivariate model and obtained summary point estimates of sensitivity and specificity with 95% CIs.ResultsTwenty-eight trials and 38270 patients were included in the meta-analysis. Most of the study settings were in the intensive care units. The overall risk of bias was high. The pooled sensitivity of the P/F ratio in all included studies for a P/F ratio of 100 was 43.6% (95% CI, 36.9-50.5%) and the specificity was 71.1% (95% CI, 66.7-75.1%) and those for a P/F ratio of 200 were 83.2% (95% CI, 78.2-87.2%) and 26.2% (95% CI, 21.2-31.9%). ConclusionThe P/F ratio had high sensitivity and moderate specificity at a P/F ratio of 200 and 100 respectively., which supports the use of the P/F ratio for screening ARDS patients who are at risk of deterioration.Trial registration: The study was registered in UMIN with registration number 000041058.
Multispectral Active Infrared Thermo-Reflectometry for Absolute Temperature Measurement by Contactless IR Thermography
Authors: Lafargue-Tallet Thomas , VAUCELLE Romain , CALIOT Cyril , AOUALI Abderezak , ABISSET-CHAVANNE Emmanuelle , SOMMIER Alain , PEIFFER Raymond , PRADERE Christophe
Published: November 2021

Knowledge of material emissivity maps and their true temperatures is of great interest for contactless process monitoring and control with infrared cameras when strong heat transfer and temperature change are involved.In this work, we describe the development of a contactless infrared and multispectral imaging technique based on the pyro-reflectometry approach and a specular model of the material reflection.This approach enables in situ and real-time identification of emissivity fields and autocalibration of the radiative intensity leaving the sample by using a black body equivalent ratio.This is done to obtain the absolute temperature field of any specular material using the infrared wavelength.The proposed method is evaluated at room temperature with several heterogeneous samples covering a large range of emissivity values. From these emissivity fields, raw and heterogeneous measured radiative fluxes are transformed into complete absolute temperature fields.
Depression among an Immigrant Black Population of African Origin resident in Canada
Authors: Aluh Deborah , Aigbogun Osaro , Anyachebelu Obinna
Published: November 2021

Background

Lately, there has been a surge of black African-born immigrants to Canada. It is critical to evaluate the extent to which depression has affected this vulnerable and understudied population.
Methods

Participants completed the Patient Health Questionnaire (PHQ-9) depression scale. Descriptive and multivariate logistic regression analyses were carried out using IBM SPPS.
Results

About half (51.7%, n = 91) of the participants met the criteria for depression. Female participants had a significantly higher PHQ-9 score (10.49±4.226) compared to males (8.96± 4.119). Unmarried participants had 27.979 times the odds of being depressed compared to those who were married. Those who had stayed in Canada for more than 10 years had 62.5 times higher odds of being depressed compared to those who had stayed for less than one year.
Conclusions

More than half of the participants exhibited significant depressive symptoms, suggesting an important mental health concern and the need for intervention.
Extracorporeal Perfusion for Free Fillet Lower Leg Flap After External Hemipelvectomy for Recurrent Osteosarcoma: A New Surgical Technique
Authors: Andreani Lorenzo , Mani Olimpia , Ipponi Edoardo , Cosseddu Fabio , Cigna Emanuele , Capanna Rodolfo
Published: November 2021

BackgroundExternal hemipelvectomy often results in exposure of noble structures such as pelvic organs, joint surfaces, iliac neurovascular bundle and bone. Consequently, reconstructive surgery is always challenging. The free fillet lower leg flap is an optimal solution since it allows the transfer of a large amount of tissue and the it determines the absence of donor site morbidities. The prolonged ischemic time represents the weak point of this flap.Methods We presented the case of a 38-year-old man with recurrent radio-induced osteosarcoma arisen on pelvic cavity and involving common iliac vessels, bladder, left ureter and left kidney. We decided to use extracorporeal circulation (ECMO) in order to reduce ischemic time of the flap, since iliac vessels were necessary ligated in the middle of oncologic resection. We perfused the popliteal-based filleted lower leg musculocutaneous free flap with homologue blood and saline solution, while the oncologic dissection was completed.ResultsThe free fillet flap remained vital in the immediate postoperative period and in the following months. The patient did not come back to a life-threatening condition at 1y follow-up. ConclusionWe believed that ECMO expand the reconstructive indications in those cases in which a complex and long-lasting oncologic resection would make impossible a free flap due to prolonged ischemic time.
PRPF6 Induces the Alternative Splicing of lncRNA SNHG16 To Promote Progression and Paclitaxel Resistance of Ovarian Cancer via Regulating GATA3 Transcription
Authors: Wang Han , Zhou Yingying , Zhang Siyang , Qi Ya , Wang Min
Published: November 2021

Background

Small nucleolar RNA host gene 16 (SNHG16) and pre-mRNA processing factor 6(PRPF6) play vital roles in regulatory mechanisms of multiple cancers, but the mechanisms in ovarian cancer (OC) remains poorly understood.
Methods

The expression of SNHG16 transcripts-SNHG16-L/S in OC tissues were analyzed by real-time PCR (RT-PCR). The expression of PRPF6 in OC tissues were detected by Immunohistochemistry (IHC). Tumorigenesis, epithelial-to-mesenchymal transition (EMT) and PTX-resistance were detected by western blot, transwell, CCK-8 assays, colony formation assays and flow cytometry analyses. Molecular interactions were examined by dual-luciferase reporter gene assay, RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP).
Results

The results indicated the expression of SNHG16-L/S was opposite in chemo-resistance and chemo-sensitivity tissues of OC. And SNHG16-L/S had different effects on the progression and PTX-resistance of OC cells. SNHG16-L inhibited GATA binding protein 3 (GATA3) transcription through CCAAT/enhancer-binding protein b (CEBPB) to further promote tumorigenesis, EMT and PTX-resistance of OC. Moreover, PRPF6 was upregulated in chemo-resistance tissues of OC. PRPF6 promoted tumorigenesis and PTX-resistance in vitro and in vivo. Mechanistically, PRPF6 induced the alternative splicing of SNHG16 to downregulate SNHG16-L, which further mediated progression and PTX-resistance through upregulating GATA3 in OC.
Conclusions

Totally, the results demonstrated that PRPF6 promoted progression and PTX-resistance in OC through SNHG16-L/CEBPB/GATA3 axis. Thus, PRPF6 may become a valuable target for OC therapy.
The structure-activity relationship between precursor fine structure and cathodes performance in Ni-rich layered oxide
Authors: Qiu Lang , Zhang Mengke , Hu Kanghui , Song Yang , Wu Zhenguo , Yue Luchao , Xiang Wei , Ming Yong , Wang Gong-Ke , Liu Yu-Xia , Sun Yan , Zhang Jun , Zhang Bin , Guo Xiaodong
Published: November 2021

Cathode’s primary particle structure plays a key role in the performance of lithium ion batteries, which can be controlled by the precursor synthesis. Regretfully, the relevance between primary particle structure and cathode performance is not explicitly elucidated, that is, what is the discrepancy of cathode’s primary particle size on the structural degradation? In order to elaborate the structure-activity relationship between them, we have systematically investigated the regulation of primary particle size through an in-depth analysis of the precursor growth mechanism, ammonia-stirring coupling and hydrodynamics optimization. Structural and electrochemical characterizations of LiNi0.92Co0.04Mn0.04O2 with different primary sizes (336, 447, 565 and 675 nm) and a rounded analysis of structural degradation after cycling provide insight into the correlation between precursor fine structure and cathode performance, i.e. larger cathode’s primary particle size can effectively inhibit CEI film formation, structure decay, the intragranular/intergranular cracks formation owing to the alleviation of localized stress.
Utility of Three-Protein Panels in the Separation of Aggressive Prostate Cancer from Non-Aggressive Tumors
Authors: Li Qing Kay , Lih Tung-shing Mamie , Wang Yuefan , Hu Yingwei , Hoti Naseruddin , Chan Daniel , Zhang Hui
Published: November 2021

Background

Prostate cancer (PCa) is a heterogeneous group of tumors, including non-aggressive (NAG) and aggressive (AG) subtypes, with variable clinical outcomes. We assessed the diagnostic utility of selected protein markers to identify AG tumors.
Methods

The TMA was constructed, including NAG and AG. 12 protein markers were evaluated using the TMA by IHC stains. The makers were also evaluated for their potential utility as single or panels for distinguishing AG from NAG tumors.
Results

The higher expressions of four protein markers, including prostate specific membrane antigen (PSMA), phospho-EGFR, androgen receptor (AR), and P16, were identified in AG tumors of Gleason score 4 and 5. In contrast, Galectin-3, DPP4 and MAN1B1 revealed stronger staining patterns in NAG tumors. Sensitivity and specificity of individual marker varied widely. In tow-marker panels, especially in the panel of DPP4 and PSMA, the specificity was 38.46% at 95% sensitivity. To further improve the detection ability, we combined DPP4 and PSMA with either Galectin-3 or phospho-EGFR into three-marker panels. The specificity achieved >46% at 95% sensitivity and AUC was >0.85.
Conclusions

Our panels can be used to improve the separation of AG from NAG tumor and to add in the optimization of the treatment strategy for patients.