bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the Application of Data Mining Technology in College Students’ Mental Health Education in the Network Age
Authors: Li Xianghai
Published: March 2022

As the marcher of life in the times, the network has penetrated into all fields of life and played an immeasurable role. As a specific contemporary group, college students have a weak sense of self-protection and are very vulnerable to adverse factors. Some colleges and universities are trying to explore various psychological intervention modes in order to conduct psychological counselling and prediction more objectively. Network mental education means that, on the premise of using social networks, educators use psychological science methods to exert a positive impact on all aspects of students' mental health education, so as to promote the development of contemporary mental health of college students under social networks and cultivate the correct mentality of using social networks. It is combined with the established college students' rational correlation analysis system. Based on the collected data and basic information of college students' rational evaluation, the improved mining algorithm is used, and some rules and characteristics of college students' psychological related factors are analyzed from the results, which provides a new idea for college students' rational health education. Aiming at realizing the embedded data mining technology in the psychological management system, this paper expounds in detail the design and implementation of the data mining technology module suitable for the psychological management system and discusses the factors affecting students' mental health.
Cimifugin Ameliorates Lipotoxicity-Induced Hepatocyte Damage and Steatosis through TLR4/p38 MAPK- and SIRT1-Involved Pathways
Authors: Yang Wenwen , Zhu Linwensi , Lai Shanglei , Ding Qinchao , Xu Tiantian , Guo Rui , Dou Xiaobing , Chai Hui , Yu Zhiling , Li Songtao
Published: March 2022

Objective. Hepatic metabolic disorder induced by lipotoxicity plays a detrimental role in metabolic fatty liver disease pathogenesis. Cimifugin (Cim), a coumarin derivative extracted from the root of Saposhnikovia divaricata, possesses multiple biological properties against inflammation, allergy, and oxidative stress. However, limited study has addressed the hepatoprotective role of Cim. Here, we investigate the protective effect of Cim against lipotoxicity-induced cytotoxicity and steatosis in hepatocytes and clarify its potential mechanisms. Methods. AML-12, a nontransformed mouse hepatocyte cell line, was employed in this study. The cells were incubated with palmitate or oleate to imitate hepatotoxicity or steatosis model, respectively. Results. Cim significantly reversed palmitate-induced hepatocellular injury in a dose-dependent manner, accompanied by improvements in oxidative stress and mitochondrial damage. Cim pretreatment reversed palmitate-stimulated TLR4/p38 MAPK activation and SIRT1 reduction without affecting JNK, ERK1/2, and AMPK pathways. The hepatoprotective effects of Cim were abolished either through activating TLR4/p38 by their pharmacological agonists or genetical silencing SIRT1 via special siRNA, indicating a mechanistic involvement. Moreover, Cim treatment improved oleate-induced hepatocellular lipid accumulation, which could be blocked by either TLR4 stimulation or SIRT1 knockdown. We observed that SIRT1 was a potential target of TLR4 in palmitate-treated hepatocytes, since TLR4 agonist LPS aggravated, whereas TLR4 antagonist CLI-095 alleviated palmitate-decreased SIRT1 expression. SIRT1 knockdown did not affect palmitate-induced TLR4. In addition, TLR4 activation by LPS significantly abolished Cim-protected SIRT1 reduction induced by palmitate. These results collaboratively indicated that TLR4-regulated SIRT1 pathways was mechanistically involved in the protective effects of Cim against lipotoxicity. Conclusion. In brief, we demonstrate the protective effects of Cim against lipotoxicity-induced cell death and steatosis in hepatocytes. TLR4-regulated p38 MAPK and SIRT1 pathways are involved in Cim-protected hepatic lipotoxicity. Cim is a potential candidate for improving hepatic metabolic disorders mediated by lipotoxicity.
Empirical Research on the Prediction Effect of Volatility Model Based on the Perspective of Investor Sentiment Health and Market Liquidity
Authors: Yang Wenqi , Yang Bing , Yang Chenzi
Published: March 2022

Healthy investor sentiment has an important impact on stock market volatility. To avoid extreme volatility in the stock market, it becomes crucial to focus on the sentiment health of investors. Therefore, we establish the realized volatility model, the implied volatility model, and the historical volatility model, respectively, introduce investor sentiment and market liquidity into these models, and empirically study the forecast effects of future daily and weekly frequency volatility to play a role in the development of the physical industry and the improvement of organizational performance. The study found that the HAR-RV model has the strongest predictive ability, while the GJR-GARCH model’s predictive effect is not ideal; market liquidity and high investor sentiment can improve the predictive effect of most models; compared with long-term (one-week) volatility, the effect of predicting short-term (one-day) volatility is better.
The Catastrophe Analysis of Shanghai Crude Oil Futures Price from the Perspective of Volatility Factors
Authors: Gong Weifeng , Li Yahui , Wang Chuanhui , Zhang Haixia , Zhai Zhengjie
Published: March 2022

The volatility of Shanghai crude oil futures prices is researched in this paper. The cusp catastrophe analysis of Shanghai crude oil futures price is based on the perspective of volatility influencing factors. Some important factors are selected based on commodity attributes and financial attributes, including certain China’s macrofactors, such as producer price index and macroeconomic prosperity index. The principal component analysis is used to process the factors. The control variables of the cusp catastrophe model are extracted. The discriminant of the cusp catastrophe model and the principal component analysis are combined to determine the month of mutation. The three‐dimensional renderings and plane projections of Shanghai crude oil futures price mutations in 2018 and 2019 are presented. The model is tested by comparing the time points of price sudden change nodes and emergencies. The results show that only nine factors can be used to roughly determine whether the Shanghai crude oil futures price has a sudden change. During the analysis period, the mutation months are May 2018, December 2018, February 2019, June 2019, and September 2019. It can be proved that the established model has certain feasibility and accuracy.
The Impact of a Knowledge Discovery-Based Psychoanalytic Intervention in the Treatment of Tuberculosis in University Students with Different Doses of Isoniazid
Authors: Xia Zhihui , Tan Youping , Yang Yumei
Published: March 2022

Tuberculosis (TB) is an infectious disease that poses a serious threat to the health of the population in China, and TB outbreaks in universities have aroused great concern in society. Psychological emotions have a large impact on the academic lives of university students, and nowadays it is not only labour-intensive but also slow to monitor and analyse and deal with the psychology of university students’ daily lives in a uniform manner. If psychological problems are not detected and given feedback in a timely manner, they can have a series of negative effects on the individual university student. In this paper, we apply the Bi-LSTM model and the CNN model neural network algorithm to learn the text data, and finally have 95.55% and 90.03% accuracy in the sentiment analysis experiment, respectively, which provides a feasible solution to solve the batch rapid analysis of the psychological changes reflected in the daily text of university students. Risk communication for TB emergencies should emphasize public participation, timely release of information about the epidemic, and good monitoring of public opinion.
Expression of LINC00847 in Peripheral Blood Mononuclear Cells of Children with Asthma and Its Prediction between Asthma Exacerbation and Remission
Authors: Hu Jiaying , Wang Zhike , Han Suzhen , Chen Kai
Published: March 2022

Objective. Asthma is defined as a heterogeneous disease that is usually characterized by chronic airway inflammation. Long noncoding RNAs play important roles in various biological processes including inflammation. To know more about the relationships between lncRNAs and asthma, we sought to the role of LINC00847 in peripheral blood mononuclear cells (PBMCs) of children with asthma exacerbation or asthma remission. Methods. Microarray analysis was performed on GSE143192 and GSE165934 datasets to screen differentially expressed lncRNAs (DElncRNAs) in human PBMCs between asthma patients and normal controls. LINC00847 was selected from DElncRNAs in human PBMCs between asthma patients and normal controls for further investigation. The expression levels of LINC00847 were quantified in PBMCs collected from 54 children with asthma exacerbation, 54 children with asthma remission, and 54 healthy children by real-time qPCR. The forced expiratory volume in the first second in percent predicted values (FEV1%), ratio of forced expiratory volume in 1 second to forced vital capacity (FEV1/FVC), and peak expiratory flow rate (PEF%) were tested for evaluation of lung function. The concentration of immunoglobulin E (IgE) and eosinophil count was examined. The serum levels of interleukin-4 (IL-4), interferon-γ (IFN-γ), and IL-17A were determined by the ELISA method. Results. The expression level of LINC00847 in PBMCs of asthma exacerbation children was remarkably higher than that in PBMCs of asthma remission children and healthy children (

p
<
0.001

); the expression level of LINC00847 in PBMCs of asthma remission children was notably higher than that in PBMCs of healthy children (

p
<
0.001

). Pearson correlation analysis revealed that the expression levels of LINC00847 in PBMCs of asthma children were negatively correlated with FEV1% (r = −0.489), FEV1/FVC (r = −0.436), PEF% (r = −0.626), and IFN-γ level (r = −0.614) of asthma children, but positively correlated with IgE concentration (r = 0.680), eosinophil count (r = 0.780), IL-4 (r = 0.524), and IL-17A (r = 0.622) levels. When LINC00847 expression was used to distinguish asthma exacerbation from asthma remission, a 0.871 AUC (95% CI: 0.805–0.936) was yielded with sensitivity of 79.63% and specificity of 77.78%. Conclusion. The study demonstrates that increased LINC00847 expression may be associated with the development and progression of asthma, possibly serving as a novel biomarker for predicting asthma exacerbation from asthma remission.
Emodin-Mediated Treatment of Acute Kidney Injury
Authors: Liu Yu , Li Mingquan , Teh LaiKuan , Zhao Liangbin , Ye Naijing , Wu Ling , Wu Lihua
Published: March 2022

Acute kidney injury (AKI), a common condition associated with a high mortality rate, is characterized by declined glomerular filtration rate, retention of nitrogen products, and disturbances in balance of water, electrolyte, and acid-base. Up to date, there is no effective treatment for AKI. Despite the continuous improvement in blood purification techniques, a considerable proportion of patients with AKI still progress to end-stage renal disease. These patients with advanced stage of end-stage renal disease will require long-term renal replacement therapy, which places a heavy burden on the family and the society. In recent years, the use of traditional Chinese medicine (TCM) in AKI management has been gradually increasing. Clinical evidence has demonstrated that three-month treatment with TCM produced better clinical outcomes in terms of clinical effectiveness rate and improvement in renal function (serum creatinine, neutrophil gelatinase-associated lipocalin, and cystatin C) compared with Western medicine. Rhubarb is a commonly used herb in TCM for the treatment of AKI. The main active component of rhubarb is emodin, which was first recorded in Shennong’s Classic of Materia Medica. It has been shown that emodin has a variety of pharmacological activities including antibacterial, anti-inflammatory, antiulcer, and immunosuppressive effects. Emodin has been found to be effective against renal fibrosis and has been widely studied for its effects on kidney diseases such as diabetic nephropathy, renal fibrosis, and AKI. Moreover, promising results have been obtained from these studies. In this study, the results obtained from research on the use of emodin for AKI treatment has been reviewed.
Observation on the Effect of Intelligent Machine-Assisted Surgery and Perioperative Nursing
Authors: Lei Liping
Published: March 2022

Orthopedic surgery and care during the perioperative period are the key to the treatment of orthopedic diseases, which can quickly and effectively treat orthopedic diseases and can quickly recover during the perioperative period. Therefore, this paper focuses on the observation of the effect of intelligent machine-assisted surgery and perioperative care, combined with smart wearable devices and C-arm camera calibration; the details of the bone surgery are assisted by the machine, and then the recognition ability is accelerated by writing into the digital bone bank. Based on machine vision, CNN training and learning are designed to design a machine-assisted perioperative nursing method. This paper also designed a bone surgery test experiment and perioperative adverse event data analysis, combined with the data obtained from the experiment, designed a comparison experiment with traditional surgery and perioperative nursing. The experimental results show that the success rate of machine-assisted surgery is increased by nearly 2%–15% compared with traditional surgery; and the rehabilitation degree of machine-assisted perioperative nursing is 15.83% higher than that of traditional perioperative nursing.
Application and Research of Gangue Partial-Filling Mining Method in Preventing Water Inrush from Floor
Authors: Pang Zhenzhong , Zhang Xinguo , Chen Juntao , Li Feifan
Published: March 2022

Coal gangue produced during coal production not only poses a serious threat to the ground environment but also imposes serious economic burdens on the mine. The partial-filling mining (PFM) method proposed in this paper can make full use of coal gangue and is of great significance to the prevention and control of water disasters at the working face. The specific process used to implement this method is to first divide the working face into several narrow working faces and then fill the filling body into part of the goaf. The ability of PFM to restrain floor water inrush is analyzed by physical simulation, and the field application research is carried out at the No. 9211 mining face of Bucun Coal Mine in Shandong, China. The physical simulation results show that the failure depth of this layer is less than 5 m. The field measurements reveal that the maximum compression deformation of the filling body is 89.1 mm, and the maximum floor failure depth of the floor is only 8.6 m. Comparative analysis indicates that the floor failure depth of the No. 9211 working face with the local filling method is 4.6 m lower than that of the No. 9110 working face with the strip mining method. In addition, no water inrush accident occurs at the No. 9211 working face during mining. Therefore, PFM not only controls the floor damage depth effectively but also consumes coal gangue to protect the mine environment.
Isolation, Identification, Characterization, and Plasmid Profile of Urinary Tract Infectious Escherichia coli from Clinical Samples
Authors: Thangavelu Sathiamoorthi , Dhandapani Ranjithkumar , Arulprakasam Ajucarmelprecilla , Paramasivam Ragul , Chinnathambi Arunachalam , Ali Alharbi Sulaiman , Durairaj Kaliannan , Shrestha Anupama
Published: March 2022

Objective. In recent times, urinary tract infection (UTI) is one of the most widely recognized bacterial diseases all over the planet. UTI influences individuals of any age and gender. The target of this study is to concentrate on the recurrence of uropathogens, the antimicrobial susceptibility pattern of the isolates, and the plasmid profile of people from the government clinics of Karaikudi. Methods. From July 2017 to December 2017, 100 urine tests were gathered and handled for the isolation of pathogenic microbes. In total, 89 isolates were found from the samples collected. Results. Escherichia coli was discovered as the most common bacterial isolate screened from the UTI-infected people, accounting for 28.09 percent of all isolates. E. coli was seen to be the highest prevalent bacterium for UTI in all age groups and demonstrated resistance to routinely used medications, especially cefpodoxime and novobiocin, which have been 100 percent resistant. The E. coli isolates screened were positive for beta-lactamase and film generation, and they have strong antimicrobial resistance. As a result, the E. coli strains with the highest prevalence of virulence determinants have become more resistant to many medications because they support the microorganism in overcoming the host’s defense and colonizing or entering the urinary system. The amplified 16S rRNA product was analyzed, and phylogenetic relationships were determined. The presence of TEM (56 percent), CTX-M (64 percent), SHV (40 percent), and OXA (60 percent) was discovered. Among E. coli isolates, CTX-M was the most common extended spectrum-beta lactamase (ESBL). Multiplex PCR was also used to identify the existence of CTX-M subgroups in E. coli isolates. Conclusion. Finally, we urge that antibiotic selection should be predicated on the awareness of the specific prevalence and that novel antimicrobial medicines for urinary infections be developed to combat the overuse of antibiotics.
Efficacy and Safety of Re Du Ning Injection for Acute Exacerbations of Chronic Obstructive Pulmonary Disease: A Systematic Review and Meta-Analysis
Authors: Shu Bofei , Li Hong , Zhou Xu , Ding Zhaohui , Wan Liling
Published: March 2022

Background. Re Du Ning, a traditional Chinese medicine injection, has been widely used for the treatment of chronic obstructive pulmonary disease, although without established systematic review evidence. This systematic review aimed to assess the efficacy and safety of Re Du Ning in the treatment of acute exacerbations of chronic obstructive pulmonary disease (AECOPD). Methods. We searched seven databases (PubMed, Embase, the Cochrane Library, SinoMed, CNKI, WanFang, and the Chinese Clinical Trial Registry) up to November 1, 2021, to identify randomized controlled trials of Re Du Ning for AECOPD. Two researchers independently carried out literature screening and data extraction. Effects were measured by risk ratios (RRs) or mean differences (MDs) with 95% confidence intervals (CIs). The meta-analysis was completed by RevMan 5.4 software. Results. Twenty-six studies met the eligibility criteria, with a total of 2284 patients. The findings of the meta-analysis indicated that the response rate of the experimental group was higher than that of the control group: RR = 1.14% and 95% CI: (1.09, 1.19). Significantly greater improvements in pulmonary function: FEV1: MD = 0.28 L, 95% CI: (0.20, 0.36); FEV1/FVC: MD = 8.63%, 95% CI: (4.68, 12.59); T-lymphocyte counts: CD4: MD = 6%, 95% CI: (2.44, 9.56); CD3: MD = 10.42%, 95% CI: (8.6, 12.24); CD4/CD8: MD = 0.38%, 95% CI: (0.32, 0.43); acid/base imbalance: PH: MD = 0.05, 95% CI: (0.01, 0.10); PaO2: MD = 9.02 mmHg, 95% CI: (11.11, 0.10),

p
=
0.005

; C-reactive protein: MD = −6.65 mg/L, 95% CI: (−10.97, −2.34); and PCT: MD = −0.28 μg/L, 95% (CI: −0.41, −0.15) were observed in patients receiving Re Du Ning compared with those receiving the control treatment. Re Du Ning did not significantly change the carbon dioxide partial pressure. All reported adverse reactions were mild. Conclusion. Re Du Ning injection, as a complementary therapy to routine treatment, has better efficacy than Western medicine alone in relieving clinical symptoms, delaying pulmonary function decline, and improving inflammation indicators for AECOPD, with good safety. The evidence was limited by a lack of high-quality RCTs.
Comparison of Ocular Morphological Parameters Related to Lens Position by Anterior Segment Optical Coherence Tomography and Ultrasound Biomicroscopy
Authors: Yu Zhiying , Wang Fenglei , Dong Fang , Li Na , Wang Dabo , Wang Ling
Published: March 2022

Objective. The objective is to compare parameters related to lens position measured using anterior segment optical coherence tomography (AS-OCT) and ultrasound biomicroscopy (UBM) in patients with senile cataract and perform a consistency analysis. Methods. This prospective study included 102 patients (102 eyes) scheduled for simple cataract surgery. Among the total patients, 44 were men, and 58 were women. AS-OCT (sitting) and UBM (lying) were used to measure the anterior chamber depth (ACD) in horizontal and vertical orientations and the iris-lens contact distance (ILCD) and iris-lens angle (ILA) in inferior, superior, nasal, and temporal quadrants. Paired-sample t-test was used to compare ACD, ILCD, and ILA measurements of the two methods, while Pearson’s linear correlation and Bland-Altman analyses were used to analyze the correlation and consistency of the two results. Results. The horizontal (2.499 ± 0.464 mm) and vertical (2.531 ± 0.463 mm) ACD measured using AS-OCT and the horizontal (2.556 ± 0.467 mm) and vertical (2.563 ± 0.479 mm) ACD measured using UBM were significantly different (

P
<
0.001

); moreover, the results showed good correlation and agreement. A significant difference was observed between the two methods in terms of ILCD measured in inferior, superior, nasal, and temporal quadrants (

P
<
0.001

), and a significant correlation was found between measurements of both methods (

P
<
0.001

). Approximately 3.92% (4/102), 0.98% (1/102), 3.92% (4/102), and 2.94% (3/102) of points were outside the 95% limits of agreement in the four quadrants, respectively, and the agreement of the results was good. ILA measured using both methods differed in inferior, superior, nasal, and temporal quadrants (

P
=
0.003

, 0.011, 0.001, 0.001, respectively), and the correlation was good (

P
<
0.001

). The percentage of points outside the 95% limit was higher in inferior, superior, nasal, and temporal quadrants (4.90% (5/102), 5.88% (6/102), 5.88% (6/102), and 6.86% (7/102)) with poor agreement of the results. Conclusions. The correlation between AS-OCT and UBM in terms of measuring lens position-related parameters was good, but the agreement was unstable. The differences in measurement position (sitting and supine) and/or measurement methods (optics and ultrasound) may lead to variability in results.
A Systems Engineering Approach for the Design of an Omnidirectional Autonomous Guided Vehicle (AGV) Testing Prototype
Authors: Tejada Juan C. , Toro-Ossaba Alejandro , Muñoz Montoya Santiago , Rúa Santiago
Published: March 2022

This paper addresses the mechanical and electrical design of an autonomous guided vehicle (AGV) test prototype based on a systems engineering approach. First, the different phases of the systems engineering approach are described. The conceptual design begins with the house of quality, which weighs the relevance of each user requirement and ends with a functional representation of the vehicle. Then, the mechanical and electrical design are presented considering different subsystems such as the chassis, cargo platform, suspension system, power, and control components. Finally, different tests were carried out on the prototype, validating its movement and load capacities. The systems engineering approach as a methodology for the construction of complex systems has proven to be an excellent tool for the development of autonomous guided vehicles.
Obesity as a Prognostic Factor of Central Nervous System Relapse in Children with Acute Lymphoblastic Leukemia: A Single-Centre Study and Literature Review
Authors: He Guo-qian , Dai Yi-ling , Jiang Ming-yan , Gao Ju , Guo Xia
Published: March 2022

Relapse as the commonest treatment failure through chemotherapy of child presented with acute lymphoblastic leukemia (ALL) is usually within 3 years of remission. Central nervous system (CNS) is expected as a site of extramedullary relapse in 3–8% of child leukemia, often leading to a poor prognosis. A few patients may have headache and vomiting and can be diagnosed without difficulty. However, most patients present with asymptomatic conditions. Obesity has become one of the greatest reported complications of children ALL survivors. Rarely, obesity presentation can be the first manifestation of CNS leukemia. Here, we present three unusual cases with B-ALL presentation of obesity as the first symptom at the time of CNS relapse after achieving remission. This highly localized presentation is unusual and would hopefully inform clinicians to have a high index of suspicion for relapse in children with ALL.
The Effects of Task-Based Speaking Tasks on Iranian University Students Willingness to Communicate and Self-Efficacy in Online Classes: A Mixed-Methods Study
Authors: Rahimi Siamak
Published: March 2022

This mixed-methods study attempts to explore the effects of TBLT on the development of Iranian university students' self-efficacy and willingness to communicate in speaking skills. To this end, two intact classes were selected in Ayatollah Borujerdi University in Borujerd City, Iran. The experimental group went through an eight-week teaching program, whereas the control group received the usual content-based instruction. The data were gathered from multiple sources at various time points using two questionnaires and a focus group interview. The results of the quantitative analysis using ANCOVA revealed a positive effect of the online TBLT approach on university students' self-efficacy and willingness to communicate in speaking in the Iranian context. Moreover, the findings of the focus group interview disclosed several themes about the merits of TBLT on self-efficacy and willingness to communicate in oral interaction skills. In the end, a range of implications is suggested for the different stakeholders.
GSPE Protects against Bleomycin-Induced Pulmonary Fibrosis in Mice via Ameliorating Epithelial Apoptosis through Inhibition of Oxidative Stress
Authors: Sul Ok Joo , Kim Jin Hyoung , Lee Taehoon , Seo Kwang Won , Cha Hee Jeong , Kwon Byungsuk , Ahn Jong-Joon , Cho You Sook , Oh Yeon-Mok , Jegal Yangjin , Ra Seung Won
Published: March 2022

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive interstitial lung disease of unknown cause which leads to alveolar epithelial cell apoptosis followed by basement membrane disruption and accumulation of extracellular matrix, destroying the lung architecture. Oxidative stress is involved in the development of alveolar injury, inflammation, and fibrosis. Oxidative stress-mediated alveolar epithelial cell (AEC) apoptosis is suggested to be a key process in the pathogenesis of IPF. Therefore, the present study investigated whether grape seed proanthocyanidin extract (GSPE) could inhibit the development of pulmonary fibrosis via ameliorating epithelial apoptosis through the inhibition of oxidative stress. We found that GSPE significantly ameliorated the histological changes and the level of collagen deposition in bleomycin (BLM)-induced lungs. Moreover, GSPE attenuated lung inflammation by reducing the total number of cells in bronchoalveolar lavage (BAL) fluid and decreasing the expression of IL-6. We observed that the levels of H2O2 leading to oxidative stress were increased following BLM instillation, which significantly decreased with GSPE treatment both in vivo and in vitro. These findings showed that GSPE attenuated BLM-induced epithelial apoptosis in the mouse lung and A549 alveolar epithelial cell through the inhibition of oxidative stress. Furthermore, GSPE could attenuate mitochondrial-associated cell apoptosis via decreasing the Bax/Bcl-2 ratio. The present study demonstrates that GSPE could ameliorate bleomycin-induced pulmonary fibrosis in mice via inhibition of epithelial apoptosis through the inhibition of oxidative stress.
Deep Learning-Based Destination Prediction Scheme by Trajectory Prediction Framework
Authors: Yang Jingkang , Cao Jianyu , Liu Yining
Published: March 2022

Destination prediction is an important task in vehicular ad hoc networks (VANETs), which benefits to resolve traffic congestion. The traditional destination prediction methods are mainly based on statistical learning, which is difficult to fit the movement pattern of vehicles and predict destination of a new query trajectory. In recent years, deep learning attracted more and more attentions since it has a better ability to adaptively extract features from data and fit complex models. The exiting deep learning-based destination prediction methods mainly extract features from past trajectory and fail to exploit future trajectory because the future locations are unknown, which results in a large prediction error. In this paper, we propose a novel deep learning-based destination prediction model that can predict the future trajectory and make full use of the information about it. Firstly, we exploit a trajectory prediction method to predict future locations, which makes the process of destination prediction continuous. Secondly, we design a branch network to determine which future location is the destination of the trajectory. We also evaluate our model with a real-world dataset. Experimental results show that our model can predict the coordinates of destination with a low prediction error.
Application of Data Fusion and Image Video Teaching Mode in Physical Education Course Teaching and Evaluation of Teaching Effect
Authors: Zou Wei , Li Yanlong , Shan Xinru , Wu Xinge
Published: March 2022

The technology and functions of data fusion and image teaching are constantly improving with the development of society and are gradually produced in life in order to adapt to the progress of science and technology. Data fusion technology refers to the information processing technology that uses computer to automatically analyze and synthesize several observation information obtained in time series under certain criteria to complete the required decision-making and evaluation tasks. In this paper, the traditional teaching mode on students' learning and other aspects of help is not very obvious, and video teaching method can not only increase students' interest in learning, but also help their learning is very huge. Teaching video is to make the knowledge, skills, and other content that teachers want to impart to students into video form to assist modern multimedia teaching. In this paper, two classes with the same number and similar average test scores were selected as the experimental objects. The test results are evaluated by f-s-o algorithm, f-s-o matrix, variance proportion method, and teaching evaluation, so as to get more accurate results. In the experiment, two classes with 30 students were selected, and different teaching methods were adopted for the two classes. One class adopted the traditional teaching mode, and the other class used the video teaching method. During the 4-month experiment, there was no significant difference in test scores and comprehension between the two classes in the first month. The ratio of the two classes was 5 : 3 in the second month, 10 : 5 in the third month, and 20 : 7 in the fourth month. Through the comparative evaluation of the test results of four months, the experimental results show that the video teaching method can not only mobilize the enthusiasm of students but also plays a very important role in promoting students' performance.
Analytical Threshold for Chaos of Piecewise Duffing Oscillator with Time-Delayed Displacement Feedback
Authors: Ming-Xin Zhang , Jun Wang , Yong-Jun Shen , Jian-Chao Zhang
Published: March 2022

In this paper, the bifurcation and chaotic motion of a piecewise Duffing oscillator with delayed displacement feedback under harmonic excitation are studied. Based on the Melnikov method, the necessary critical conditions for the chaotic motion in the system are obtained, and the chaos threshold curve is obtained by calculation and numerical simulation. The accuracy of the analytical result is proved by some typical numerical simulation results, including the local bifurcation diagrams, phase portraits, Poincaré maps, and the largest Lyapunov exponents. The effects of excitation frequency and time delay of the displacement feedback are analytically discussed. It could be found that the critical excitation amplitude will increase obviously with the increase of the excitation frequency, and under the selection of certain parameters, the critical excitation amplitude takes the time delay of 0.58 as the inflection point, which decreases at first and then increases.
Levetiracetam-Induced Psychosis in the Setting of Intracranial Cavernomas
Authors: Majarwitz Daniel , Dvalishvili Mariam , Pastis Irene
Published: March 2022

Levetiracetam is a commonly used antiepileptic medication that has been associated with the development of psychosis. Cavernomas are vascular malformations that have been associated with psychosis as well, especially in the setting of hemorrhagic transformation. This case report describes a patient with cavernomas who developed psychotic symptoms after restarting her levetiracetam at a high dose (2000 mg twice a day) without gradual uptitration. Her symptoms improved upon the reduction of the levetiracetam as well as the initiation of paliperidone. This case highlights the importance of considering the biologic and medication-related factors for the development of psychosis, as well as the importance of gradual medication adjustments.
Upregulation of Yin-Yang-1 Associates with Proliferation and Glutamine Metabolism in Esophageal Carcinoma
Authors: Luo Can , Chen Xin , Bai Yuting , Xu Lei , Wang Shuqi , Yao Lihua , Guo Xiaolan , Wang Dongsheng , Zhong Xiaowu
Published: March 2022

Objective. To investigate the expression of Yin-Yang-1 (YY1) in esophageal carcinoma (ESCA) and its effect on glutamine metabolism in ESCA. Methods. The expression and roles of YY1 in ESCA were investigated using a series of bioinformatics databases and tools. The expression of YY1 between ESCA tissues with the corresponding adjacent tissues was validated using real-time PCR, western blot, and immunohistochemical staining method. Furthermore, the effects of YY1 on ESCC cell proliferation and migration were examined. The correlation between the YY1 and glutamine metabolism was evaluated by western blot. Results. YY1 gene was highly conserved in evolution and upregulated in ESCA tissues and ESCC cell lines (ECA109 and TE-1). In addition, YY1 may affect the level of immune cell infiltration and promote tumor cell immune escape. Functional enrichment analysis found that YY1 involved in many biological processes, such as cell division and glutathione and glutamine metabolism. After siRNA knockdown of YY1 in ECA109 and TE-1, the proliferation and the migration of ECA109 and TE-1 were suppressed. The glutamine consumption and glutamate production were significantly decreased. The protein expression of alanine-, serine-, cysteine-preferring transporter 2 (ASCT2), glutaminase (GLS), and glutamate dehydrogenase (GLUD1) was significantly downregulated. Conclusion. YY1 is highly expressed in ESCA and may promote glutamine metabolism of ESCC cells, indicating it may be as a diagnostic biomarker for ESCA.
Phosphorylation of SHP2 at Tyr62 Enables Acquired Resistance to SHP2 Allosteric Inhibitors in FLT3-ITD–Driven AML
Authors: Pfeiffer Anamarija , Franciosa Giulia , Locard-Paulet Marie , Piga Ilaria , Reckzeh Kristian , Vemulapalli Vidyasiri , Blacklow Stephen C. , Theilgaard-Mönch Kim , Jensen Lars J. , Olsen Jesper V.
Published: March 2022

The protein tyrosine phosphatase SHP2 is crucial for oncogenic transformation of acute myeloid leukemia (AML) cells expressing mutated receptor tyrosine kinases. SHP2 is required for full RAS-ERK activation to promote cell proliferation and survival programs. Allosteric SHP2 inhibitors act by stabilizing SHP2 in its autoinhibited conformation and are currently being tested in clinical trials for tumors with overactivation of the RAS/ERK pathway, alone and in various drug combinations. In this study, we established cells with acquired resistance to the allosteric SHP2 inhibitor SHP099 from two FLT3-ITD (internal tandem duplication)-positive AML cell lines. Label-free and isobaric labeling quantitative mass spectrometry–based phosphoproteomics of these resistant models demonstrated that AML cells can restore phosphorylated ERK (pERK) in the presence of SHP099, thus developing adaptive resistance. Mechanistically, SHP2 inhibition induced tyrosine phosphorylation and feedback-driven activation of the FLT3 receptor, which in turn phosphorylated SHP2 on tyrosine 62. This phosphorylation stabilized SHP2 in its open conformation, preventing SHP099 binding and conferring resistance. Combinatorial inhibition of SHP2 and MEK or FLT3 prevented pERK rebound and resistant cell growth. The same mechanism was observed in a FLT3-mutated B-cell acute lymphoblastic leukemia cell line and in the inv(16)/KitD816Y AML mouse model, but allosteric inhibition of Shp2 did not impair the clonogenic ability of normal bone marrow progenitors. Together, these results support the future use of SHP2 inhibitor combinations for clinical applications.

Significance:

These findings suggest that combined inhibition of SHP2 and FLT3 effectively treat FLT3-ITD–positive AML, highlighting the need for development of more potent SHP2 inhibitors and combination therapies for clinical applications.
Phase II Study of Ramucirumab in Advanced Biliary Tract Cancer Previously Treated By Gemcitabine-Based Chemotherapy
Authors: Lee Sunyoung , Shroff Rachna T. , Makawita Shalini , Xiao Lianchun , Danner De Armas Anaemy , Bhosale Priya , Reddy Kavitha , Shalaby Ahmed , Raghav Kanwal , Pant Shubham , Wolff Robert A. , Javle Milind
Published: March 2022

Purpose:

VEGF receptor-2 (VEGFR-2)–mediated angiogenesis contributes to pathogenesis of biliary tract cancers (BTC). We investigated ramucirumab, a mAb targeting VEGFR-2 for treatment of advanced, chemorefractory BTC.

Patients and Methods:

This is a phase II, single-arm trial for advanced, unresectable, pre-treated patients with BTC with ECOG 0/1, adequate liver, renal, and marrow functions. Ramucirumab was administered at 8 mg/kg, 2 weekly with restaging performed 8 weekly. Primary endpoint was progression-free survival (PFS). Secondary endpoints were overall response rate (ORR), disease control rate (DCR), overall survival (OS), and toxicity. Exploratory endpoints included correlation of tumor mutational status with PFS and OS.

Results:

61 patients were enrolled: the median age was 58.5 years; 59 with stage IV disease; 62%, intrahepatic cholangiocarcinoma; 22%, gallbladder cancer; and 16%, extrahepatic cholangiocarcinoma. All received prior chemotherapy: 52% had 1 prior, and rest ≥2 prior lines. Median treatment duration was 10.1 weeks (range, 2.1–86). Median PFS was 3.2 months [95% confidence interval (CI), 2.1–4.8]; median OS, 9.5 months (95% CI, 5.8–13.6). One (1.7%) patient achieved partial response; 26 (43.3%), stable disease; and 25 (41.7%), disease progression; DCR, 45%. Median 6-month PFS and OS rates were 32% (95% CI, 0.22–0.46) and 58% (95% CI, 0.47–0.72). The majority of toxicities were grade 1 or 2; grade 3 proteinuria (1, 2%), hypertension (13, 22%), and pulmonary embolism (1, 2%), and grade 4 gastrointestinal bleeding (1, 2%) occurred.

Conclusions:

Ramucirumab was well tolerated and resulted in PFS similar to that achieved with other chemotherapy regimens used historically for chemorefractory BTC.
VCAM-1–targeted MRI Improves Detection of the Tumor-brain Interface
Authors: Cheng Vinton W.T. , de Pennington Nicholas , Zakaria Rasheed , Larkin James R. , Serres Sébastien , Sarkar Manjima , Kirkman Matthew A. , Bristow Claire , Croal Paula , Plaha Puneet , Campo Leticia , Chappell Michael A. , Lord Simon , Jenkinson Michael D. , Middleton Mark R. , Sibson Nicola R.
Published: March 2022

Purpose:

Despite optimal local therapy, tumor cell invasion into normal brain parenchyma frequently results in recurrence in patients with solid tumors. The aim of this study was to determine whether microvascular inflammation can be targeted to better delineate the tumor-brain interface through vascular cell adhesion molecule-1 (VCAM-1)-targeted MRI.

Experimental Design:

Intracerebral xenograft rat models of MDA231Br-GFP (breast cancer) brain metastasis and U87MG (glioblastoma) were used to histologically examine the tumor-brain interface and to test the efficacy of VCAM-1–targeted MRI in detecting this region. Human biopsy samples of the brain metastasis and glioblastoma margins were examined for endothelial VCAM-1 expression.

Results:

The interface between tumor and surrounding normal brain tissue exhibited elevated endothelial VCAM-1 expression and increased microvessel density. Tumor proliferation and stemness markers were also significantly upregulated at the tumor rim in the brain metastasis model. T2*-weighted MRI, following intravenous administration of VCAM-MPIO, highlighted the tumor-brain interface of both tumor models more extensively than gadolinium-DTPA–enhanced T1-weighted MRI. Sites of VCAM-MPIO binding, evident as hypointense signals on MR images, correlated spatially with endothelial VCAM-1 upregulation and bound VCAM-MPIO beads detected histologically. These findings were further validated in an orthotopic medulloblastoma model. Finally, the tumor-brain interface in human brain metastasis and glioblastoma samples was similarly characterized by microvascular inflammation, extending beyond the region detectable using conventional MRI.

Conclusions:

This work illustrates the potential of VCAM-1–targeted MRI for improved delineation of the tumor-brain interface in both primary and secondary brain tumors.
Artificial Intelligence-Assisted Serial Analysis of Clinical Cancer Genomics Data Identifies Changing Treatment Recommendations and Therapeutic Targets
Authors: Fischer Catherine G. , Pallavajjala Aparna , Jiang LiQun , Anagnostou Valsamo , Tao Jessica , Adams Emily , Eshleman James R. , Gocke Christopher D. , Lin Ming-Tseh , Platz Elizabeth A. , Xian Rena R.
Published: March 2022

Purpose:

Given the pace of predictive biomarker and targeted therapy development, it is unknown whether repeat annotation of the same next-generation sequencing data can identify additional clinically actionable targets that could be therapeutically leveraged. In this study, we sought to determine the predictive yield of serial reanalysis of clinical tumor sequencing data.

Experimental Design:

Using artificial intelligence (AI)-assisted variant annotation, we retrospectively reanalyzed sequencing data from 2,219 patients with cancer from a single academic medical center at 3-month intervals totaling 9 months in 2020. The yield of serial reanalysis was assessed by the proportion of patients with improved strength of therapeutic recommendations.

Results:

A total of 1,775 patients (80%) had ≥1 potentially clinically actionable mutation at baseline, including 243 (11%) patients who had an alteration targeted by an FDA-approved drug for their cancer type. By month 9, the latter increased to 458 (21%) patients mainly due to a single pan-cancer agent directed against tumors with high tumor mutation burden. Within this timeframe, 67 new therapies became available and 45 were no longer available. Variant pathogenicity classifications also changed leading to changes in treatment recommendations for 124 patients (6%).

Conclusions:

Serial reannotation of tumor sequencing data improved the strength of treatment recommendations (based on level of evidence) in a mixed cancer cohort and showed substantial changes in available therapies and variant classifications. These results suggest a role for repeat analysis of tumor sequencing data in clinical practice, which can be streamlined with AI support.
Porcupine Inhibition Disrupts Mitochondrial Function and Homeostasis in WNT Ligand–Addicted Pancreatic Cancer
Authors: Aguilera Kristina Y. , Le Thuc , Riahi Rana , Lay Anna R. , Hinz Stefan , Saadat Edris A. , Vashisht Ajay A. , Wohlschlegel James , Donahue Timothy R. , Radu Caius G. , Dawson David W.
Published: March 2022

WNT signaling promotes pancreatic ductal adenocarcinoma (PDAC) through diverse effects on proliferation, differentiation, survival, and stemness. A subset of PDAC with inactivating mutations in ring finger protein 43 (RNF43) show growth dependency on autocrine WNT ligand signaling and are susceptible to agents that block WNT ligand acylation by Porcupine O-acyltransferase, which is required for proper WNT ligand processing and secretion. For this study, global transcriptomic, proteomic, and metabolomic analyses were performed to explore the therapeutic response of RNF43-mutant PDAC to the Porcupine inhibitor (PORCNi) LGK974. LGK974 disrupted cellular bioenergetics and mitochondrial function through actions that included rapid mitochondrial depolarization, reduced mitochondrial content, and inhibition of oxidative phosphorylation and tricarboxylic acid cycle. LGK974 also broadly altered transcriptional activity, downregulating genes involved in cell cycle, nucleotide metabolism, and ribosomal biogenesis and upregulating genes involved in epithelial–mesenchymal transition, hypoxia, endocytosis, and lysosomes. Autophagy and lysosomal activity were augmented in response to LGK974, which synergistically inhibited tumor cell viability in combination with chloroquine. Autocrine WNT ligand signaling dictates metabolic dependencies in RNF43-mutant PDAC through a combination of transcription dependent and independent effects linked to mitochondrial health and function. Metabolic adaptations to mitochondrial damage and bioenergetic stress represent potential targetable liabilities in combination with PORCNi for the treatment of WNT ligand-addicted PDAC.
Cigarette Smoke Containing Acrolein Contributes to Cisplatin Resistance in Human Bladder Cancers through the Regulation of HER2 Pathway or FGFR3 Pathway
Authors: Hong Jian-Hua , Tong Zhen-Jie , Wei Tung-En , Lu Yu-Chuan , Huang Cheng-Yu , Huang Chao-Yuan , Chiang Chih-Hung , Jaw Fu-Shan , Cheng Hsiao-Wei , Wang Hsiang-Tsui
Published: March 2022

Cisplatin-based chemotherapy is the first-line therapy for bladder cancer. However, cisplatin resistance has been associated with the recurrence of bladder cancer. Previous studies have shown that activation of FGFR and HER2 signaling are involved in bladder cancer cell proliferation and drug resistance. Smoking is the most common etiologic risk factor for bladder cancer, and there is emerging evidence that smoking is associated with cisplatin resistance. However, the underlying mechanism remains elusive. Acrolein, a highly reactive aldehyde, is abundant in tobacco smoke, cooking fumes, and automobile exhaust fumes. Our previous studies have shown that acrolein contributes to bladder carcinogenesis through the induction of DNA damage and inhibition of DNA repair. In this study, we found that acrolein induced cisplatin resistance and tumor progression in both non–muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC) cell lines RT4 and T24, respectively. Activation of HER2 and FGFR3 signaling contributes to acrolein-induced cisplatin resistance in RT4 and T24 cells, respectively. Furthermore, trastuzumab, an anti-HER2 antibody, and PD173074, an FGFR inhibitor, reversed cisplatin resistance in RT4 and T24 cells, respectively. Using a xenograft mouse model with acrolein-induced cisplatin-resistant T24 clones, we found that cisplatin combined with PD173074 significantly reduced tumor size compared with cisplatin alone. These results indicate that differential molecular alterations behind cisplatin resistance in NMIBC and MIBC significantly alter the effectiveness of targeted therapy combined with chemotherapy. This study provides valuable insights into therapeutic strategies for cisplatin-resistant bladder cancer.