bluuebunny/crossref_metadata_2025 huggingface dataset:
Mastery is central: an examination of complex interrelationships between physical health, stress and adaptive cognition, and social connection with depression and anxiety symptoms
Authors: Shin Huiyoung , Park Chaerim
Published: May 2024

Background

Research has established the link between physical health, stress and cognition, and social connection with depression and anxiety. Nevertheless, an understanding of the comorbidity of depression and anxiety symptoms and their complex interrelationships with relevant factors remains still limited. This study investigated the complex pattern of interplay between depression and anxiety symptoms and pertinent physical, cognitive, and social factors and potential gender differences.

Methods

Using a sample of 600 middle-aged men and women, depression and anxiety as well as physical health, perceived stress and mastery, and social connection were assessed. The network structure of depression and anxiety symptoms and risk and resilience factors were characterized by examining interrelationships and the centrality indices of Strength and Bridge Strength. Gender differences were examined using the Network Comparison Test.

Results

Perceived stress and mastery were central bridge factors influencing comorbid depression and anxiety symptoms, and perceived stress, anhedonia, and mastery exhibited strong inter-connections to each other. The connections of physical health-anhedonia and sleep disturbance-irritability were stronger in men than in women, while social connection with family was linked to interpersonal problems only in women.

Conclusions

The results underscore that prevention and interventions targeting reducing perceptions of stress and promoting mastery would prevent onset or recurrence of depression and anxiety symptoms among middle-aged men and women. Engaging in behavioral activities to maintain physical health and ensuring adequate sleep could be particularly beneficial for men in reducing overall symptom severity.
Knowledge level and health information-seeking behavior of people with diabetes in rural areas: a multicenter cross-sectional study
Authors: Wang Yudong , Zhang Yanping , Guo Tingting , Han Jiaxia , Fu Guifen
Published: May 2024

Introduction

There is a lack of research on the current level of diabetes knowledge and health information-seeking behaviors among patients with diabetes in rural areas of China’s economically underdeveloped regions during COVID-19, as well as a lack of up-to-date evidence on glycemic control and the incidence of complications among rural patients with diabetes.

Objectives

To investigate the prevalence of glycemic control and complications among patients with diabetes in rural areas, to explore the current status and correlation of diabetes knowledge level and health information-seeking behavior, and to analyze the factors affecting diabetes knowledge level.

Methods

From January 2022 to July 2022, we conducted a screening on diabetic complications and a questionnaire survey among 2,178 patients with diabetes in 15 county hospitals in rural areas of Guangxi Zhuang Autonomous Region. The patients’ knowledge level and health information-seeking behavior were investigated. Spearman correlation analysis was used to assess the correlation between diabetes knowledge and health information-seeking behavior. Multiple linear regression analysis was used to test how demographic information and health information-seeking behavior influenced the level of diabetes knowledge.

Results

Of 2,178 patients with diabetes in rural areas, 1,684 (77.32%) had poor glycemic control, and the prevalence of diabetic complications was estimated to be 72.13%. Patients with diabetes had poor diabetes knowledge and health information-seeking behavior, and there is a strong positive correlation between them. Diabetes knowledge level was influenced by per capita household disposable income, occupational status, gender, age, ethnicity, family history of diabetes, insulin use, glycated hemoglobin, education level, number of complications and health information-seeking behavior.

Conclusion

Patients with diabetes in rural areas have poor glycemic control and a high incidence of diabetic complications. Patients with diabetes in rural areas have poor knowledge and inadequate health information-seeking behavior. Systematic and standardized education should be provided to improve patients’ diabetes knowledge and thus improve their self-management ability.
The effect of Ashtanga-Vinyasa Yoga method on air force pilots’ operational performance
Authors: Santos Sara , Melo Filipe , Fernandes Orlando , Parraca José Alberto
Published: May 2024

Introduction

In today’s military landscape, optimizing performance and bolstering physical health and mental resilience are critical objectives. Introducing a 12-week Ashtanga Vinyasa Yoga Supta Method (AVYSM) to the training protocol of military trained Airforce pilots, we aim to assesses the feasibility and impact of the method.

Materials and equipment

Borg Scale assesses the intensity level of physical activity during the intervention. Flight simulator data gauges operational performance responses. Postural control responses are measured using a force platform, stress responses are monitored via heart monitor, and handgrip dynamometry will measure strength. Respiratory capacity is assessed using a spirometer, body composition is evaluated using impedance balance, and aviation-related questionnaires are administered before and after the intervention period.

Methods

In a randomized controlled trial, the totality of pilots from the “Masters in Military Aeronautics: aviator pilot specialist” course at the Portuguese Air Force Academy (PAA) were randomly assigned to the yoga intervention or the waiting list control groups, with participants providing written informed consent. The control group followed protocolized course classes for 12 weeks, while the intervention group integrated two weekly one-hour yoga sessions into their course.

Results

The PAA has approved the implementation of this intervention protocol at Airbase 11 in Beja, highlighting its significance for the organization’s policy makers. We hypothesize that this method will enhance operational performance and, subsequently, elevate flight safety.

Discussion

This research’s potential extends beyond the PAA, as it can be adapted for use in Airforce departments of other nations and various military contexts.

Clinical trial registration

Évora University research ethics committee—approval number 21050. Study registered on ClinicalTrials.gov under identifier NCT05821270, registered on April 19, 2023.
Cleaning Up After Ourselves: The Role of Carbon Dioxide Removal
Authors: Fuss Sabine , Jones Chris D. , Schulte Ingrid , Vaughan Naomi
Published: May 2024

The latest science shows that, to stop global warming, we must reach “net zero” emissions by 2050. This means that any carbon dioxide (CO2) humans are still releasing into the atmosphere in 2050 would need to be balanced out by taking CO2 from the atmosphere and locking it away. The process of removing CO2 from the atmosphere is called carbon dioxide removal (CDR). This article will explain why CDR is needed and will introduce some methods of removing CO2, including the strengths and weaknesses of those methods. There are many actions we can take. Many bring lots of other benefits, while some have drawbacks too. But overall, Carbon Dioxide Removal has a big role to play in successfully tackling climate change.
Gender Equality in Diastasis Rectus Abdominis in Chronic Back Pain: A Model of M. Transversus Abdominis Motor Control Impairment
Authors: van Wingerden J. P. , Ronchetti I. , Kleinrensink G-J.
Published: May 2024

Introduction: Diastasis rectus abdominis (DRA) is defined as an increased distance between the left and right muscle of the m. rectus abdominis. Pregnancy-related factors are assumed to be dominant factors in the occurrence of DRA. However DRA is not only found in peri-partum women but also in men and nulliparous women with back or pelvic pain. This study provides an inventory of the incidence of DRA in subjects with chronic back and pelvic pain. If DRA is common in both men and women then other factors besides pregnancy, like impaired motor control, should be explored as cause for DRA.

Material and Methods: This study was conducted with data from 849 back pain patients. Results from ultrasound assessment of the abdominal wall were combined with anamnestic data on age, gender, medical history and pregnancies (in women).

Results: There was no difference in Inter Rectus Distance cranial of the umbilicus (IRD above umbilicus) between men and women. Almost half of all women and men (45% and 43%, respectively) exhibit an increased IRD above umbilicus. The incidence of an increased IRD above umbilicus is twice as high in women below 30 years, compared to men below 30 years old. This difference is not observed for men and women above 30 years old.

Discussion: DRA occurs in women during pregnancy and increases with an increasing number of pregnancies. However, this condition does not affect significantly more women than men. Increased IRD above umbilicus already occurs in young men (mean age 30). Over 30 years of age, cranial of the umbilicus there is no difference in IRD between women and men. An alternative etiological mechanism is suggested.
The Relationship Between Work-Related Stress and Depression: A Scoping Review
Authors: du Prel Jean-Baptist , Koscec Bjelajac Adrijana , Franić Zrinka , Henftling Lorena , Brborović Hana , Schernhammer Eva , McElvenny Damien M. , Merisalu Eda , Pranjic Nurka , Guseva Canu Irina , Godderis Lode
Published: May 2024

Objectives

Work-related stress is highly prevalent. Recent systematic reviews concluded on a significant association between common work-related stress measures and depression. Our scoping review aims to explore whether work-related psychosocial stress is generally associated with depression or depressiveness, the extent and methodology of the primary research undertaken on this topic and to elucidate inconsistencies or gaps in knowledge.

Methods

We searched for literature in Pubmed, PsycInfo and Web of Science including full reports in seven languages published between 1999 and 2022 and applied the PRISMA statement for scoping reviews criteria.

Results

Of 463 primarily identified articles, 125 were retained after abstract and full-text screening. The majority report significant associations between work-related stress and depression. Cross-sectional studies are most prevalent. Sufficient evidence exists only for job strain and effort-reward imbalance. Most studies are from Asia, North America and Europe. The health sector is the most studied. Several research gaps such as the lack of interventional studies were identified.

Conclusion

The consistency of most studies on the significant association between work-related stress and depression is remarkable. More studies are needed to improve evidence and to close research gaps.
Meeting the Shortage of Human Cells and Tissues: The Andalusian Quality Assurance Programme for Tissue Donation
Authors: Alvarez-Marquez Antonia José , Huet Jesus , Pérez-Villares José Miguel , Daga-Ruiz Domingo , Diaz-Aunión Concepción , Castro de la Nuez Pablo , Cuende Natividad
Published: May 2024

Background A quality assurance programme for the tissue donation process was launched in Andalusia in 2020 to facilitate the integration of tissue donation into end-of-life care, and to respond to the growing need for human tissue for therapeutic purposes. The results of this programme are presented here.

Methods After identifying the hospital departments in which to intensify the detection of tissue donors, expanding training activities and designing a specific data collection system for possible tissue donors who do not donate their tissues, the results of the donation activity were quantified and the causes of non-donation were analysed by applying the critical pathway for deceased tissue donation methodology.

Results After an initial drop in activity, which coincided with the coronavirus pandemic, the number of tissue donors increased by 48.4% in 2022 compared to 2019. From the eligible donors, 83% were actual tissue donors and 71% were utilised donors. The modifiable causes of tissue donation loss, in order of frequency, were family refusal, followed by organisational or logistical issues, failure to notify or failure to identify possible donors, and failure to complete donor evaluation.

Conclusion As a result of the collaboration of the various professionals involved in the programme, tissue donation activity has increased remarkably, the potential and effectiveness of the donation process have been evaluated, and areas for improvement have been identified, which we hope will lead to continuous improvement of the process.
Pre-Transplant Calcimimetic Use and Dose Information Improves the Accuracy of Prediction of Tertiary Hyperparathyroidism after Kidney Transplantation: A Retrospective Cohort Study
Authors: Okada Manabu , Sato Tetsuhiko , Himeno Tomoki , Hasegawa Yuki , Futamura Kenta , Hiramitsu Takahisa , Ichimori Toshihiro , Goto Norihiko , Narumi Shunji , Watarai Yoshihiko
Published: May 2024

Tertiary hyperparathyroidism (THPT) is characterized by elevated parathyroid hormone and serum calcium levels after kidney transplantation (KTx). To ascertain whether pre-transplant calcimimetic use and dose information would improve THPT prediction accuracy, this retrospective cohort study evaluated patients who underwent KTx between 2010 and 2022. The primary outcome was the development of clinically relevant THPT. Logistic regression analysis was used to evaluate pre-transplant calcimimetic use as a determinant of THPT development. Participants were categorized into four groups according to calcimimetic dose, developing two THPT prediction models (with or without calcimimetic information). Continuous net reclassification improvement (CNRI) and integrated discrimination improvement (IDI) were calculated to assess ability to reclassify the degree of THPT risk by adding pre-transplant calcimimetic information. Of the 554 patients, 87 (15.7%) developed THPT, whereas 139 (25.1%) received pre-transplant calcimimetic treatment. Multivariate logistic regression analysis revealed that pre-transplant calcimimetic use was significantly associated with THPT development. Pre-transplant calcimimetic information significantly improved the predicted probability accuracy of THPT (CNRI and IDI were 0.91 [p < 0.001], and 0.09 [p < 0.001], respectively). The THPT prediction model including pre-transplant calcimimetic information as a predictive factor can contribute to the prevention and early treatment of THPT in the era of calcimimetics.
Research on Damping Hole Optimization of Hydro-Pneumatic Suspension for Mining Trucks under Variable Load Conditions
Authors: Zhu Wenfeng , Zhao Zenglu , Zhou Xingtong , Cao Xuepeng , Ye Min , Cao Chuqing , Alam Mohammad Manjur
Published: May 2024

The hydro-pneumatic suspension, known for enhancing vehicle ride comfort and stability, finds widespread use in engineering vehicles. Presently, the majority of mining trucks employ hydro-pneumatic suspension with a fixed damping hole, underscoring the critical importance of selecting appropriate damping hole parameters. Initially, an equilibrium mathematical model of the ¼ hydro-pneumatic suspension is established, and the influencing factors of the damping characteristics are analyzed. Subsequently, the simulation model and experimental bench for the hydro-pneumatic suspension are constructed. Sinusoidal signals with different frequencies and amplitudes serve as the excitation signals to analyze the variation trend of the force on the rod with displacement changes. The simulation and experimental results demonstrate a high degree of consistency, validating the rationality and validity of the simulation model. Building upon this foundation, various damping apertures are then selected to study the damping characteristics of the hydro-pneumatic suspension. The research indicates that as the damping aperture increases, the setting time of the hydro-pneumatic suspension system after excitation extends, accompanied by a decrease in the acceleration overshoot. As a result, a comprehensive evaluation index is developed, considering various factors, such as different weight setting times and peak longitudinal accelerations to assess the ride comfort of the suspension. This approach is then employed to determine the optimal damping aperture under both full-load and no-load conditions. The findings of this research offer valuable insights for the development of adaptive variable damping hydraulic suspensions, especially under variable load conditions.
A Tube-Based Model Predictive Control for Path Tracking of Autonomous Articulated Vehicle
Authors: Lee Taeyeon , Jeong Yonghwan
Published: May 2024

This paper presents tube-based Model Predictive Control (MPC) for the path and velocity tracking of an autonomous articulated vehicle. The target platform of this study is an autonomous articulated vehicle with a non-steerable axle. Consequently, the articulation angle and wheel torque input are determined by the tube-based MPC. The proposed MPC aims to achieve two objectives: minimizing path tracking error and enhancing robustness to disturbances. Furthermore, the lateral stability of the autonomous articulated vehicle is considered to reflect its dynamic characteristics. The vehicle model for the MPC is formulated using local linearization to minimize modeling errors. The reference state is determined using a virtual controller based on the linear quadratic regulator to provide the optimal reference for the MPC solver. The proposed algorithm was evaluated through a simulation study with base algorithms under noise injection into the sensor signal. Simulation results demonstrate that the proposed algorithm achieved the smallest path tracking error, compared to the base algorithms. Additionally, the proposed algorithm demonstrated robustness to external noise for multiple signals.
Study on Dynamics of Overrunning Spring Clutches and Suppression Methods for Their Abnormal Noise
Authors: Zhou Jie , Qiu Zhehang , Zhang Huijuan , Zhan Jianming
Published: May 2024

Overrunning spring clutches are widely used as essential transmission devices, and the occurrence of abnormal noise can lead to a decline in their performance. This study investigates the dynamic aspects of abnormal noise in engineering applications, including its causes, influencing factors, and suppression methods. Audio processing algorithms are employed to analyze the audio associated with abnormal noise, and the Fourier Motion Blur algorithm is applied to process video images of the springs. By combining the motion blur curve with the noise spectrum curve, the source of the abnormal noise is identified as friction-induced vibrations in the spring. Theoretical modeling and calculations are carried out from a dynamic perspective to validate that the phenomenon of abnormal noise in the clutch is a result of self-excited friction vibration caused by the stick–slip phenomenon. Based on theoretical analysis and practical engineering, surface texturing is added to the center shaft of the spring seat, optimizing the system as an overdamped system to suppress self-vibration. Utilizing CFD simulation analysis, the simulation results are used to improve the texturing parameters and further optimize the texturing shape, resulting in an optimal parallelogram surface texture structure. Experimental validation confirms that the improved overrunning spring clutch completely eliminates abnormal noise during overrunning operation. Therefore, this paper contributes to the understanding of the dynamic issues associated with abnormal noise in overrunning spring clutches, confirming that the mechanism for abnormal noise generation is friction-induced self-excitation vibration, and demonstrating that surface texture optimization methods effectively suppress the occurrence of abnormal noise.
Development of a Universal Adaptive Control Algorithm for an Unknown MIMO System Using Recursive Least Squares and Parameter Self-Tuning
Authors: La Hanbyeol , Oh Kwangseok
Published: May 2024

This study proposes a universal adaptive control algorithm for an unknown multi-input multi-output (MIMO) system using recursive least squares (RLS) and parameter self-tuning. The issue of adjusting the control and system parameters in response to changes in the platform was discussed. The development of a control algorithm that can consistently achieve reliable and robust control performance in various systems is important. This study aimed to develop a control algorithm that can track the reference value for any unknown MIMO system. For the controller design, an nth-order differential error dynamic model was designed, and an RLS with a scale factor was used to estimate the coefficients of the error dynamics. In the current scenario, the numbers of control inputs and error states in the error dynamics were assumed to be equal. It was designed such that the control input is derived based on the Lyapunov stability concept using the estimated coefficients. The scale factor in the RLS and injection term in the control input based on the sliding-mode approach were computed using a self-tuning methodology. The performance of the proposed universal adaptive control algorithm was evaluated using an actual DC motor and CarMaker (version 8.1.1) software tests under various scenarios.
Sensor-Based Identification of Singularities in Parallel Manipulators
Authors: Pulloquinga Jose L. , Ceccarelli Marco , Mata Vicente , Valera Angel
Published: May 2024

Singularities are configurations where the number of degrees of freedom of a robot changes instantaneously. In parallel manipulators, a singularity could reduce the mobility of the end-effector or produce uncontrolled motions of the mobile platform. Thus, a singularity is a critical problem for mechanical design and model-based control. This paper presents a general sensor-based method to identify singularities in the workspace of parallel manipulators with low computational cost. The proposed experimental method identifies a singularity by measuring sudden changes in the end-effector movements and huge increments in the forces applied by the actuators. This paper uses an inertial measurement unit and a 3D tracking system for measuring the end-effector movements, and current sensors for the forces exerted by the actuators. The proposed sensor-based identification of singularities is adjusted and implemented in three different robots to validate its effectiveness and feasibility for identifying singularities. The case studies are two prototypes for educational purposes—a five-bar mechanism and an L-CaPaMan parallel robot—and a four-degree-of-freedom robot for rehabilitation purposes. The tests showcase its potential as a practical solution for singularity identification in educational and industrial robots.
Current Advances in Mesenchymal Stem Cell Therapies Applied to Wounds and Skin, Eye, and Neuromuscular Diseases in Companion Animals
Authors: Picazo Rosa Ana , Rojo Concepción , Rodriguez-Quiros Jesus , González-Gil Alfredo
Published: May 2024

Mesenchymal stem cells (MSCs) are considered a very promising alternative tool in cell therapies and regenerative medicine due to their ease of obtaining from various tissues and their ability to differentiate into different cell types. This manuscript provides a review of current knowledge on the use of MSC-based therapies as an alternative for certain common pathologies in dogs and cats where conventional treatments are ineffective. The aim of this review is to assist clinical veterinarians in making decisions about the suitability of each protocol from a clinical perspective, rather than focusing solely on research. MSC-based therapies have shown promising results in certain pathologies, such as spinal cord injuries, wounds, and skin and eye diseases. However, the effectiveness of these cell therapies can be influenced by a wide array of factors, leading to varying outcomes. Future research will focus on designing protocols and methodologies that allow more precise and effective MSC treatments for each case.
Craniometric Characteristics of Selected Carnivora Species Kept in Captivity in Relation to Bite Force and Bending Strength of the Upper Canines
Authors: Różycka Katarzyna , Skibniewska Ewa , Rajkowski Łukasz , Skibniewski Michał
Published: May 2024

The aim of this study was to analyse the bite forces of seven species from three carnivore families: Canidae, Felidae, and Ursidae. The material consisted of complete, dry crania and mandibles. A total of 33 measurements were taken on each skull, mandible, temporomandibular joint, and teeth. The area of the temporalis and masseter muscles was calculated, as was the length of the arms of the forces acting on them. Based on the results, the bite force was calculated using a mathematical lever model. This study compared the estimated areas of the masticatory muscles and the bending strength of the upper canines among seven species. A strong correlation was found between cranial size and bite force. The results confirmed the hypothesis that the weight of the animal and the size of the skull have a significant effect on the bite force.
Bacitracin Methylene Disalicylate (BMD) Treatment Affects Spleen Proteome in Broiler Chicks Infected with Salmonella enteritidis
Authors: Adetunji Adedeji , Casey Theresa , Aryal Uma K. , Ogundare Tunde , Franco Jackeline , Fasina Yewande
Published: May 2024

Bacitracin Methylene Disalicylate (BMD), as a feed additive to poultry diets, enhances digestion, prevents Salmonella enteritidis (SE) colonization, and treats current infections. The objective of this study was to utilize a quantitative proteomic approach to determine the effect of BMD feed additive on broiler chickens challenged with SE in the spleen proteome. At 1 d of age, chicks were randomly allocated into four groups: control with and without SE challenge (CON, n = 60; CON-SE, n = 60), BMD with and without SE challenge (BMD, n = 60; BMD-SE, n = 60). Birds in the CON-SE and BMD-SE treatment were administered SE inoculum by oral gavage. On day three and day seven post-gavage, the spleen was collected aseptically from birds in each treatment group (CON, n = 4/day; CON-SE, n = 4/day; BMD, n = 4/day; BMD-SE, n = 4/day). Proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) showed an increased abundance of 115 proteins and decreased of 77 due to the BMD. Proteins that decreased in abundance were enriched for fibrinogen complex and extracellular space, whereas proteins that increased in abundance were enriched for proteasome-mediated ubiquitin-dependent protein catabolic process and mitochondrion. Analysis of the interaction between BMD and the Salmonella challenge found 230 differentially abundant proteins including proteins associated with RNA binding, spliceosome, protein transport, and cell adhesion among the upregulated proteins, and those associated with protein folding, carbon metabolism, biosynthesis of nucleotide sugars, response to oxidative stress, positive regulation of NIK/NF-kappaB signaling, and inflammatory response among the downregulated proteins. The impact of BMD treatment on spleen proteome indicates an anti-apoptotic effect. BMD also modified the response of the spleen to the SE challenge with a marked decrease in proteins that prompt cytokine synthesis and an increase in proteins involved in the selective removal of unfolded proteins.
Natural Antimicrobials in Dairy Products: Benefits, Challenges, and Future Trends
Authors: Soutelino Maria Eduarda Marques , Silva Adriana Cristina de Oliveira , Rocha Ramon da Silva
Published: May 2024

This review delves into using natural antimicrobials in the dairy industry and examines various sources of these compounds, including microbial, plant, and animal sources. It discusses the mechanisms by which they inhibit microbial growth, for example, by binding to the cell wall’s precursor molecule of the target microorganism, consequently inhibiting its biosynthesis, and interfering in the molecule transport mechanism, leading to cell death. In general, they prove to be effective against the main pathogens and spoilage found in food, such as Escherichia coli, Staphylococcus aureus, Bacillus spp., Salmonella spp., mold, and yeast. Moreover, this review explores encapsulation technology as a promising approach for increasing the viability of natural antimicrobials against unfavorable conditions such as pH, temperature, and oxygen exposure. Finally, this review examines the benefits and challenges of using natural antimicrobials in dairy products. While natural antimicrobials offer several advantages, including improved safety, quality, and sensory properties of dairy products, it is crucial to be aware of the challenges associated with their use, such as potential allergenicity, regulatory requirements, and consumer perception. This review concludes by emphasizing the need for further research to identify and develop effective and safe natural antimicrobials for the dairy industry to ensure the quality and safety of dairy products for consumers.
Comparative In Vitro Activity of Ceftazidime-Avibactam, Imipenem-Relebactam, and Meropenem-Vaborbactam against Carbapenem-Resistant Clinical Isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa
Authors: Sophonsri Anthony , Kalu Michelle , Wong-Beringer Annie
Published: May 2024

Co-infection with carbapenem-resistant Klebsiella pneumoniae (CRKP) and Pseudomonas aeruginosa (CRPA) is associated with poor outcomes and historically relied on combination therapy with toxic agents for management. However, several novel β-lactam/β-lactamase inhibitor combination agents have been developed, offering potential monotherapy options. Here, we compare the in vitro activity of ceftazidime-avibactam (CZA), imipenem-relebactam (IRL), and meropenem-vaborbactam (MVB) against both CRKP and CRPA clinical isolates. Minimum inhibitory concentrations (MICs) for each agent were determined using broth microdilution. Carbapenemase gene detection was performed for representative isolates of varying carbapenem resistance phenotypes. IRL demonstrated excellent activity against CRKP and CRPA with susceptibility rates at 95.8% and 91.7%, respectively. While CZA and MVB showed comparable susceptibility to IRL against CRKP (93.8%), susceptibility of CRPA to CZA was modest at 79.2%, whereas most CRPA strains were resistant to MVB. Of the 35 CRKP isolates tested, 91.4% (32/35) carried a blaKPC gene. Only 1 of 37 (2.7%) CRPA isolates tested carried a blaVIM gene, which conferred phenotypic resistance to all three agents. None of the CRKP strains were cross-resistant to all three agents. Source of infection and co-infection did not significantly influence antimicrobial activity for IRL and CZA; none of the CRPA isolates from co-infected patients were susceptible to MVB. Our results suggest that novel β-lactam agents with antipseudomonal activity and stability against carbapenemases, such as IRL and CZA, offer potential monotherapy options for the treatment of co-infection involving both CRKP and CRPA, but not MVB.
LanCL2 Implicates in Testicular Redox Homeostasis and Acrosomal Maturation
Authors: Zhao Yanling , Wang Jichen , Shi Shuai , Lan Xinting , Cheng Xiangyu , Li Lixia , Zou Yuanfeng , Jia Lanlan , Liu Wentao , Luo Qihui , Chen Zhengli , Huang Chao
Published: May 2024

Redox balance plays an important role in testicular homeostasis. While lots of antioxidant molecules have been identified as widely expressed, the understanding of the critical mechanisms for redox management in male germ cells is inadequate. This study identified LanCL2 as a major male germ cell-specific antioxidant gene that is important for testicular homeostasis. Highly expressed in the brain and testis, LanCL2 expression correlates with testicular maturation and brain development. LanCL2 is enriched in spermatocytes and round spermatids of the testis. By examining LanCL2 knockout mice, we found that LanCL2 deletion did not affect postnatal brain development but injured the sperm parameters of adult mice. With histopathological analysis, we noticed that LanCL2 KO caused a pre-maturation and accelerated the self-renewal of spermatogonial stem cells in the early stage of spermatogenesis. In contrast, at the adult stage, LanCL2 KO damaged the acrosomal maturation in spermiogenesis, resulting in spermatogenic defects with a reduced number and motility of spermatozoa. Furthermore, we show that this disruption of testicular homeostasis in the LanCL2 KO testis was due to dysbalanced testicular redox homeostasis. This study demonstrates the critical role of LanCL2 in testicular homeostasis and redox balance.
Changes in SOD and NF-κB Levels in Substantia Nigra and the Intestine through Oxidative Stress Effects in a Wistar Rat Model of Ozone Pollution
Authors: Rivas-Arancibia Selva , Rodríguez-Martínez Erika , Valdés-Fuentes Marlen , Miranda-Martínez Alfredo , Hernández-Orozco Eduardo , Reséndiz-Ramos Citlali
Published: May 2024

This work aimed to elucidate how O3 pollution causes a loss of regulation in the immune response in both the brain and the intestine. In this work, we studied the effect of exposing rats to low doses of O3 based on the association between the antioxidant response of superoxide dismutase (SOD) levels and the nuclear factor kappa light chains of activated B cells (NFκB) as markers of inflammation. Method: Seventy-two Wistar rats were used, divided into six groups that received the following treatments: Control and 7, 15, 30, 60, and 90 days of O3. After treatment, tissues were extracted and processed using Western blotting, biochemical, and immunohistochemical techniques. The results indicated an increase in 4-hydroxynonenal (4HNE) and Cu/Zn-SOD and a decrease in Mn-SOD, and SOD activity in the substantia nigra, jejunum, and colon decreased. Furthermore, the translocation of NFκB to the nucleus increased in the different organs studied. In conclusion, repeated exposure to O3 alters the regulation of the antioxidant and inflammatory response in the substantia nigra and the intestine. This indicates that these factors are critical in the loss of regulation in the inflammatory response; they respond to ozone pollution, which can occur in chronic degenerative diseases.
Hypoxic Inducible Factor Stabilization in Pericytes beyond Erythropoietin Production: The Good and the Bad
Authors: Troise Dario , Infante Barbara , Mercuri Silvia , Piccoli Claudia , Lindholm Bengt , Stallone Giovanni
Published: May 2024

The paracrine signaling pathways for the crosstalk between pericytes and endothelial cells are essential for the coordination of cell responses to challenges such as hypoxia in both healthy individuals and pathological conditions. Ischemia–reperfusion injury (IRI), one of the causes of cellular dysfunction and death, is associated with increased expression of genes involved in cellular adaptation to a hypoxic environment. Hypoxic inducible factors (HIFs) have a central role in the response to processes initiated by IRI not only linked to erythropoietin production but also because of their participation in inflammation, angiogenesis, metabolic adaptation, and fibrosis. While pericytes have an essential physiological function in erythropoietin production, a lesser-known role of HIF stabilization during IRI is that pericytes’ HIF expression could influence vascular remodeling, cell loss and organ fibrosis. Better knowledge of mechanisms that control functions and consequences of HIF stabilization in pericytes beyond erythropoietin production is advisable for the development of therapeutic strategies to influence disease progression and improve treatments. Thus, in this review, we discuss the dual roles—for good or bad—of HIF stabilization during IRI, focusing on pericytes, and consequences in particular for the kidneys.
Lemon Peel Water Extract: A Novel Material for Retinal Health, Protecting Retinal Pigment Epithelial Cells against Dynamin-Related Protein 1-Mediated Mitochondrial Fission by Blocking ROS-Stimulated Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway
Authors: Tsou Shang-Chun , Chuang Chen-Ju , Wang Inga , Chen Tzu-Chun , Yeh Jui-Hsuan , Hsu Chin-Lin , Hung Yu-Chien , Lee Ming-Chung , Chang Yuan-Yen , Lin Hui-Wen
Published: May 2024

Previous studies showed that NaIO3 can induce oxidative stress-mediated retinal pigment epithelium (RPE) damage to simulate age-related macular degeneration (AMD). Lemon peel is rich in antioxidants and components that can penetrate the blood–retinal barrier, but their role in retinal oxidative damage remains unexplored. Here, we explore the protection of lemon peel ultrasonic-assisted water extract (LUWE), containing large amounts of flavonoids and polyphenols, against NaIO3-induced retinal degeneration. We initially demonstrated that LUWE, orally administered, prevented retinal distortion and thinning on the inner and outer nuclei layers, downregulating cleaved caspase-3 protein expression in RPE cells in NaIO3-induced mice. The effect of LUWE was achieved through the suppression of apoptosis and the associated proteins, such as cleaved PARP and cleaved caspase-3, as suggested by NaIO3-induced ARPE-19 cell models. This is because LUWE reduced reactive oxygen species-mediated mitochondrial fission via regulating p-Drp-1 and Fis1 expression. We further confirmed that LUWE suppresses the expression of p-MEK-1/2 and p-ERK-1/2 in NaIO3-induced ARPE-19 cells, thereby providing the protection described above, which was confirmed using PD98059 and U0126. These results indicated that LUWE prevents mitochondrial oxidative stress-mediated RPE damage via the MEK/ERK pathway. Elucidation of the molecular mechanism may provide a new protective strategy against retinal degeneration.
Dietary Lycium barbarum Polysaccharide Modulates Growth Performance, Antioxidant Capacity, and Lipid Metabolism in Common Carp (Cyprinus carpio) Fed with High-Fat Diet
Authors: Wu Di , Li Jinnan , Fan Ze , Sun Zhipeng , Zheng Xianhu , Zhang Haitao , Xu Hong , Wang Liansheng
Published: May 2024

To investigate the ameliorative effects and mechanism of Lycium barbarum polysaccharide (LBP) on growth performance, oxidative stress, and lipid deposition in common carp (Cyprinus carpio) fed with high-fat diets, fish with an initial weight of 5.29 ± 0.12 g were divided into five experimental groups—including normal-fat diets, high-fat diets, and high-fat diets—supplemented with LBP (0.5, 1.0, and 2.0 g/kg) for 8 weeks. The results showed that high-fat diets resulted in significant decreases in final body weight, weight gain rate, and specific growth rate of fish, as well as causing a significant decrease in hepatic total antioxidant capacity, catalase, and glutathione peroxidase activities. These changes were accompanied by a significant decrease in lipase activity and ATP level and a significant increase in malondialdehyde content. The expression levels of lipid metabolism-related genes (acetyl coenzyme A carboxylase 1, stearoyl coenzyme A desaturase 1, fat synthase, peroxisome proliferator-activated receptor-γ, fructofuranose bisphosphatase, and glucose-6-phosphatase) were also markedly elevated by high-fat diets. Supplementation with 0.5–2.0 g/kg LBP in high-fat diets improved the reduced growth performance, increased hepatic total antioxidant enzymes, catalase, and glutathione peroxidase activities, and lowered malondialdehyde level in fish fed with high-fat diets. Additionally, dietary supplementation with LBP significantly downregulated hepatic gene expression levels of acetyl coenzyme A carboxylase 1, stearoyl coenzyme A desaturase 1, fat synthase, sterol regulatory element-binding protein 1, peroxisome proliferator-activated receptor-γ, fructofuranose bisphosphatase, and glucose-6-phosphatase. In conclusion, fish fed with high-fat diets demonstrated impaired growth performance, antioxidant capacity, and lipid metabolism, and dietary supplementation with 0.5–2.0 g/kg LBP ameliorated the impairments induced by high-fat diets.
Hydrogen Sulfide and Irisin, Potential Allies in Ensuring Cardiovascular Health
Authors: Flori Lorenzo , Benedetti Giada , Calderone Vincenzo , Testai Lara
Published: May 2024

Irisin is a myokine secreted under the influence of physical activity and exposure to low temperatures and through different exogenous stimuli by the cleavage of its precursor, fibronectin type III domain-containing protein 5 (FNDC5). It is mainly known for maintaining of metabolic homeostasis, promoting the browning of white adipose tissue, the thermogenesis process, and glucose homeostasis. Growing experimental evidence suggests the possible central role of irisin in the regulation of cardiometabolic pathophysiological processes. On the other side, hydrogen sulfide (H2S) is well recognized as a pleiotropic gasotransmitter that regulates several homeostatic balances and physiological functions and takes part in the pathogenesis of cardiometabolic diseases. Through the S-persulfidation of cysteine protein residues, H2S is capable of interacting with crucial signaling pathways, exerting beneficial effects in regulating glucose and lipid homeostasis as well. H2S and irisin seem to be intertwined; indeed, recently, H2S was found to regulate irisin secretion by activating the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α)/FNDC5/irisin signaling pathway, and they share several mechanisms of action. Their involvement in metabolic diseases is confirmed by the detection of their lower circulating levels in obese and diabetic subjects. Along with the importance of metabolic disorders, these modulators exert favorable effects against cardiovascular diseases, preventing incidents of hypertension, atherosclerosis, heart failure, myocardial infarction, and ischemia–reperfusion injury. This review, for the first time, aims to explore the role of H2S and irisin and their possible crosstalk in cardiovascular diseases, pointing out the main effects exerted through the common molecular pathways involved.
Inhibitory Effects of Fermented Sprouted Oat Extracts on Oxidative Stress and Melanin Overproduction
Authors: Cho Hyeijin , Yang Jisun , Kang Ji Young , Kim Kyung Eun
Published: May 2024

Hyperpigmentation occurs due to irregular secretion of melanin pigment in the skin. This can affect quality of life depending on its severity, so prevention and management are essential. Oats (Avena sativa L.), a grain consumed worldwide, are known to offer improved health benefits upon germination and fermentation. This study is aimed to investigate the protective effects of lactobacilli-fermented sprouted oat extracts on oxidative stress and melanin overproduction in vitro. The anti-melanogenic effect was investigated using melanin content and tyrosinase activity assays in B16F10 cells, as well as a mushroom tyrosinase-based enzyme inhibition assay. The results showed that L. casei-fermented oat extracts were the most effective for reducing melanin formation by reducing the mRNA expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related protein 2. Furthermore, L. casei fermentation was effective in improving the total phenolic, flavonoid, and avenanthramide A contents of sprouted oat extracts. The results also demonstrated the antioxidant effects of L. casei-fermented sprouted oat extracts in promoting DPPH radical-scavenging activity, superoxide dismutase-like activity, and reduction in reactive oxygen species levels. Overall, the findings indicate that fermented sprouted oat extracts are promising candidates for antioxidant and anti-hyperpigmentation treatments.
Exploring Immune Redox Modulation in Bacterial Infections: Insights into Thioredoxin-Mediated Interactions and Implications for Understanding Host–Pathogen Dynamics
Authors: Dagah Omer M. A. , Silaa Billton Bryson , Zhu Minghui , Pan Qiu , Qi Linlin , Liu Xinyu , Liu Yuqi , Peng Wenjing , Ullah Zakir , Yudas Appolonia F. , Muhammad Amir , Zhang Xianquan , Lu Jun
Published: May 2024

Bacterial infections trigger a multifaceted interplay between inflammatory mediators and redox regulation. Recently, accumulating evidence has shown that redox signaling plays a significant role in immune initiation and subsequent immune cell functions. This review addresses the crucial role of the thioredoxin (Trx) system in the initiation of immune reactions and regulation of inflammatory responses during bacterial infections. Downstream signaling pathways in various immune cells involve thiol-dependent redox regulation, highlighting the pivotal roles of thiol redox systems in defense mechanisms. Conversely, the survival and virulence of pathogenic bacteria are enhanced by their ability to counteract oxidative stress and immune attacks. This is achieved through the reduction of oxidized proteins and the modulation of redox-sensitive signaling pathways, which are functions of the Trx system, thereby fortifying bacterial resistance. Moreover, some selenium/sulfur-containing compounds could potentially be developed into targeted therapeutic interventions for pathogenic bacteria. Taken together, the Trx system is a key player in redox regulation during bacterial infection, and contributes to host–pathogen interactions, offering valuable insights for future research and therapeutic development.
NOX2 as a Biomarker of Academic Performance: Evidence from University Students during Examination
Authors: Nocella Cristina , D’Amico Alessandra , Cangemi Roberto , Fossati Chiara , Pigozzi Fabio , Mannacio Elena , Cammisotto Vittoria , Bartimoccia Simona , Castellani Valentina , Sarto Gianmarco , Simeone Beatrice , Rocco Erica , Frati Giacomo , Sciarretta Sebastiano , Pignatelli Pasquale , Carnevale Roberto , SMiLe Group SMiLe Group
Published: May 2024

Background: Cortisol levels, oxidative stress, and lower cerebral performance seem to be closely related. This study aimed to evaluate the question of whether exam stress affected oxidative stress and endothelial function parameters in the salivary samples of students. Methods: A total of 114 healthy students were recruited. All students were subjected to a 21-item DASS questionnaire to assess perceived stress. Cortisol levels, biomarkers of oxidative stress, and endothelial function were evaluated at T0, during the semester, and T1, in the morning before the exam, in saliva samples. In vitro, HUVECs were stimulated with cortisol, and oxidative stress and endothelial function parameters were evaluated. Results: At T1, cortisol levels were significantly increased compared with the levels during the semester. Moreover, exam results correlated inversely with the DASS score at T1. In addition, NOX2, H2O2 and endothelin-1 significantly increased, while NO bioavailability decreased. In vitro, HUVECs treatment with human cortisol determined the increase of oxidative stress and the decrease of endothelial function, in association with impaired eNOS phosphorylation. Conclusion: NOX2-mediated oxidative stress is a mechanism that could mediate cortisol-induced transient endothelial dysfunction during academic examination. Therefore, strategies to monitor or modulate oxidative stress could help students to reduce the impact of examination-related stress.