bluuebunny/crossref_metadata_2025 huggingface dataset:
Perovskite Solar Cells and Thermoelectric Generator Hybrid Array Feeding a Synchronous Reluctance Motor for an Efficient Water Pumping System
Authors: Zaky Alaa A. , Ibrahim Mohamed N. , Taha Ibrahim B. M. , Yousif Bedir , Sergeant Peter , Hristoforou Evangelos , Falaras Polycarpos
Published: July 2022

Nowadays, water pumping systems based on photovoltaics as a source of electricity have widely increased. System cost and efficiency still require enhancement in order to spread their application. Perovskite solar cells (PSCs) are the most hopeful third-generation photovoltaic for replacing the silicon-based photovoltaic thanks to their high power conversion efficiency, reaching 25.8%; tunable band-gap; long diffusion length; low fabrication temperature; and low cost. In this work, for the first time, we proposed a high-power-density hybrid perovskite solar cell thermoelectric generator (TEG) array for feeding a synchronous reluctance motor (SynRM) driving a water pump for use in an irrigation system. A control technique was used to achieve two functions. The first function was driving the motor to obtain the maximum torque/ampere. The second was harvesting the maximum perovskite solar cell array output power on the basis of the maximum power point tracking (MPPT) algorithm using the perturbation and observation approach. Thus, the proposed hybrid perovskite solar cell–thermoelectric generator feeds the motor via an inverter without DC–DC converters or batteries. Accordingly, the short life problems and the high replacement cost are avoided. The proposed complete system was simulated via the MATLAB package. Moreover, a complete laboratory infrastructure was constructed for testing the proposed high-power-density hybrid perovskite solar cell–TEG array for the water pumping system. The results revealed that using the high-power-density hybrid perovskite solar cell–TEG array, both the motor’s output power and the pump’s flow rate were improved by 11% and 14%, respectively, compared to only using the perovskite solar cell array. Finally, both the simulation and experimental results proved the high-performance efficiency of the system in addition to showing its system complexity and cost reduction.
Numerical Study of the Effect of a Heated Cylinder on Natural Convection in a Square Cavity in the Presence of a Magnetic Field
Authors: Hossain Muhammad Sajjad , Fayz-Al-Asad Muhammad , Mallik Muhammad Saiful Islam , Yavuz Mehmet , Alim Md. Abdul , Khairul Basher Kazi Md.
Published: July 2022

The present research was developed to find out the effect of heated cylinder configurations in accordance with the magnetic field on the natural convective flow within a square cavity. In the cavity, four types of configurations—left bottom heated cylinder (LBC), right bottom heated cylinder (RBC), left top heated cylinder (LTC) and right top heated cylinder (RTC)—were considered in the investigation. The current mathematical problem was formulated using the non-linear governing equations and then solved by engaging the process of Galerkin weighted residuals based on the finite element scheme (FES). The investigation of the present problem was conducted using numerous parameters: the Rayleigh number (Ra = 103–105), the Hartmann number (Ha = 0–200) at Pr = 0.71 on the flow field, thermal pattern and the variation of heat inside the enclosure. The clarifications of the numerical result were exhibited in the form of streamlines, isotherms, velocity profiles and temperature profiles, local and mean Nusselt number, along with heated cylinder configurations. From the obtained outcomes, it was observed that the rate of heat transport, as well as the local Nusselt number, decreased for the LBC and LTC configurations, but increased for the RBC and RTC configurations with the increase of the Hartmann number within the square cavity. In addition, the mean Nusselt number for the LBC, RBC, LTC and RTC configurations increased when the Hartmann number was absent, but decreased when the Hartmann number increased in the cavity. The computational results were verified in relation to a published work and were found to be in good agreement.
Therapeutic Strategies to Reduce Burn Wound Conversion
Authors: Palackic Alen , Jay Jayson W. , Duggan Robert P. , Branski Ludwik K. , Wolf Steven E. , Ansari Naseem , El Ayadi Amina
Published: July 2022

Burn wound conversion refers to the phenomenon whereby superficial burns that appear to retain the ability to spontaneously heal, convert later into deeper wounds in need of excision. While no current treatment can definitively stop burn wound conversion, attempts to slow tissue damage remain unsatisfactory, justifying the need for new therapeutic interventions. To attenuate burn wound conversion, various studies have targeted at least one of the molecular mechanisms underlying burn wound conversion, including ischemia, inflammation, apoptosis, autophagy, generation of reactive oxygen species, hypothermia, and wound rehydration. However, therapeutic strategies that can target various mechanisms involved in burn wound conversion are still lacking. This review highlights the pathophysiology of burn wound conversion and focuses on recent studies that have turned to the novel use of biologics such as mesenchymal stem cells, biomaterials, and immune regulators to mitigate wound conversion. Future research should investigate mechanistic pathways, side effects, safety, and efficacy of these different treatments before translation into clinical studies.
Sustainable and Eco-Friendly Packaging Films Based on Poly (Vinyl Alcohol) and Glass Flakes
Authors: Channa Iftikhar Ahmed , Ashfaq Jaweria , Gilani Sadaf Jamal , Chandio Ali Dad , Yousuf Sumra , Makhdoom Muhammad Atif , Jumah May Nasser bin
Published: July 2022

The majority of food packaging materials are petroleum-based polymers, which are neither easily recyclable nor ecologically friendly. Packaging films should preferably be transparent, light in weight, and easy to process, as well as mechanically flexible, and they should meet the criteria for food encapsulation. In this study, poly (vinyl alcohol) (PVA)-based films were developed by incorporating glass flakes into the films. The selection of PVA was based on its well-known biodegradability, whereas the selection of glass flakes was based on their natural impermeability to oxygen and moisture. The films were processed using the blade coating method and were characterized in terms of transparency, oxygen transmission rate, mechanical strength, and flexibility. We observed that the incorporation of glass flakes into the PVA matrix did not significantly change the transparency of the PVA films, and they exhibited a total transmittance of around 87% (at 550 nm). When the glass flakes were added to the PVA, a significant reduction in moisture permeation was observed. This reduction was also supported and proven by Bhardwaj’s permeability model. In addition, even after the addition of glass flakes to the PVA, the films remained flexible and showed no degradation in terms of the water vapor transmission rate (WVTR), even after bending cycles of 23,000. The PVA film with glass flakes had decent tensile characteristics, i.e., around >50 MPa. Increasing the concentration of glass flakes also increased the hardness of the films. Finally, a piece of bread was packaged in a well-characterized composite film. We observed that the bread packaged in the PVA film with glass flakes did not show any degradation at all, even after 10 days, whereas the bread piece packaged in a commercial polyethylene bag degraded completely. Based on these results, the developed packaging films are the perfect solution to replace commercial non-biodegradable films.
Robust Walking for Humanoid Robot Based on Divergent Component of Motion
Authors: Zhang Zhao , Zhang Lei , Xin Shan , Xiao Ning , Wen Xiaoyan
Published: July 2022

In order to perform various complex tasks in place of humans, humanoid robots should walk robustly in the presence of interference. In the paper, an improved model predictive control (MPC) method based on the divergent components of motion (DCM) is proposed. Firstly, the humanoid robot model is simplified to a finite-sized foot-pendulum model. Then, the gait of the humanoid robot in the single-support phase (SSP) and double-support phase (DSP) is planned based on DCM. The center of mass (CoM) of the robot will converge to the DCM, which simplifies the feedback control process. Finally, an MPC controller incorporating an extended Kalman filter (EKF) is proposed to realize the tracking of the desired DCM trajectory. By adjusting the step duration, the controller can compensate for CoM trajectory errors caused by disturbances. Simulation results show that—compared with the traditional method—the method we propose achieves improvements in both disturbed walking and uneven-terrain walking.
The Diagnostic Challenges and Clinical and Serological Outcome in Patients Hospitalized for Suspected Lyme Neuroborreliosis
Authors: Briciu Violeta , Flonta Mirela , Leucuța Daniel , Lupșe Mihaela
Published: July 2022

The aim of our study was to evaluate the differential diagnosis and clinical/serological outcome to antibiotic treatment in patients hospitalized for suspected Lyme neuroborreliosis (LNB). A prospective study included patients hospitalized in a Romanian hospital between March 2011 and October 2012 with neurological symptoms, positive laboratory tests for Borrelia burgdorferi, cerebrospinal fluid (CSF) analysis, and no previous treatment for LNB. A questionnaire was completed for each patient at admission, at the end of treatment, and 3 months later. Patients were treated with antibiotic therapy (ceftriaxone/cefotaxime), irrespective of CSF analysis results. A symptomatic scoring scale was used for the follow-up. Out of the 42 patients included, no patient fulfilled criteria for definite LNB; 7 patients were classified as possible LNB; and in 33 patients, LNB was excluded. Two patients could not be classified (insufficient amount of CSF). Clinical follow-up suggested a better response to therapy in the group of patients with possible LNB than in the group with LNB excluded. The patients’ differential diagnosis and serological follow-up are presented. Patients investigated for suspected LNB present diverse clinical manifestations and comorbidities that complicate differential diagnosis. LNB may be misdiagnosed if CSF analysis is not performed.
Co-Occurrence of Francisella, Spotted Fever Group Rickettsia, and Midichloria in Avian-Associated Hyalomma rufipes
Authors: Hoffman Tove , Sjödin Andreas , Öhrman Caroline , Karlsson Linda , McDonough Ryelan Francis , Sahl Jason W. , Birdsell Dawn , Wagner David M. , Carra Laura G. , Wilhelmsson Peter , Pettersson John H.-O. , Barboutis Christos , Figuerola Jordi , Onrubia Alejandro , Kiat Yosef , Piacentini Dario , Jaenson Thomas G. T. , Lindgren Per-Eric , Moutailler Sara , Fransson Thord , Forsman Mats , Nilsson Kenneth , Lundkvist Åke , Olsen Björn
Published: July 2022

The migratory behavior of wild birds contributes to the geographical spread of ticks and their microorganisms. In this study, we aimed to investigate the dispersal and co-occurrence of Francisella and spotted fever group Rickettsia (SFGR) in ticks infesting birds migrating northward in the African-Western Palaearctic region (AWPR). Birds were trapped with mist nests across the Mediterranean basin during the 2014 and 2015 spring migration. In total, 575 ticks were collected from 244 birds. We screened the ticks for the species Francisella tularensis, the genus Francisella, and SFGR by microfluidic real-time PCR. Confirmatory analyses and metagenomic sequencing were performed on tick samples that putatively tested positive for F. tularensis during initial screenings. Hyalomma rufipes was the most common tick species and had a high prevalence of Francisella, including co-occurrence of Francisella and SFGR. Metagenomic analysis of total DNA extracted from two H. rufipes confirmed the presence of Francisella, Rickettsia, and Midichloria. Average nucleotide identity and phylogenetic inference indicated the highest identity of the metagenome-assembled genomes to a Francisella-like endosymbiont (FLE), Rickettsia aeschlimannii, and Midichloria mitochondrii. The results of this study suggest that (i) FLE- and SFGR-containing ticks are dispersed by northbound migratory birds in the AWPR, (ii) H. rufipes likely is not involved in transmission of F. tularensis in the AWPR, and (iii) a dual endosymbiosis of FLEs and Midichloria may support some of the nutritional requirements of H. rufipes.
Phylotypic Diversity of Bacteria Associated with Speleothems of a Silicate Cave in a Guiana Shield Tepui
Authors: Liu Qi , He Zichen , Naganuma Takeshi , Nakai Ryosuke , Rodríguez Luz María , Carreño Rafael , Urbani Franco
Published: July 2022

The diversity of microorganisms associated with speleological sources has mainly been studied in limestone caves, while studies in silicate caves are still under development. Here, we profiled the microbial diversity of opal speleothems from a silicate cave in Guiana Highlands. Bulk DNAs were extracted from three speleothems of two types, i.e., one soft whitish mushroom-like speleothem and two hard blackish coral-like speleothems. The extracted DNAs were amplified for sequencing the V3–V4 region of the bacterial 16S rRNA gene by MiSeq. A total of 210,309 valid reads were obtained and clustered into 3184 phylotypes or operational taxonomic units (OTUs). The OTUs from the soft whitish speleothem were mostly affiliated with Acidobacteriota, Pseudomonadota (formerly, Proteobacteria), and Chloroflexota, with the OTUs ascribed to Nitrospirota being found specifically in this speleothem. The OTUs from the hard blackish speleothems were similar to each other and were mostly affiliated with Pseudomonadota, Acidobacteriota, and Actinomycetota (formerly, Actinobacteria). These OTU compositions were generally consistent with those reported for limestone and silicate caves. The OTUs were further used to infer metabolic features by using the PICRUSt bioinformatic tool, and membrane transport and amino acid metabolism were noticeably featured. These and other featured metabolisms may influence the pH microenvironment and, consequently, the formation, weathering, and re-deposition of silicate speleothems.
The Use of Probiotic Megasphaera elsdenii as a Pre-Harvest Intervention to Reduce Salmonella in Finishing Beef Cattle: An In Vitro Model
Authors: Habib Kellen , Drouillard James , de Aguiar Veloso Vanessa , Huynh Grace , Trinetta Valentina , Gragg Sara E.
Published: July 2022

Reducing Salmonella in cattle may mitigate the risk of transmission through the food chain. Megasphaera elsdenii (ME) is a microorganism found naturally in the bovine rumen that can be administered as a probiotic to mitigate ruminal acidosis. Understanding the impact of feeding ME to Salmonella populations in cattle was the objective of this study. Bovine ruminal fluid (RF) and feces were inoculated with antibiotic susceptible or resistant Salmonella and treated with varying concentrations of ME. Salmonella was enumerated at 0, 24, 48, and 72 h using the most probable number (MPN). Volatile fatty acids (VFAs) and pH were recorded from non-inoculated samples. Treating RF with ME did not significantly impact Salmonella concentration or VFA production (p > 0.05). The pH of RF and feces decreased over time (p ≤ 0.05). Salmonella concentration declined in feces, with the largest reduction of 1.92 log MPN/g and 1.05 log MPN/g observed for antibiotic susceptible Salmonella between 0 and 72 h by the 2.5 × 105 CFU/g and control (0.0 CFU/g) concentration of ME, respectively. Treating RF with ME did not impact Salmonella concentration. Salmonella concentration in feces decreased, although ME must be further investigated before a conclusion regarding efficacy in vitro can be determined.
Re-Visiting the Quantification of Hematite by Diffuse Reflectance Spectroscopy
Authors: Cao Wei , Jiang Zhaoxia , Gai Congcong , Barrón Vidal , Torrent José , Zhong Yi , Liu Qingsong
Published: July 2022

Hematite concentration is an important climatic proxy for environmental (climatic) studies of soils and sediments. However, the accurate quantification of naturally occurring hematite has always been a difficult question, especially for those areas with lower hematite concentrations. Diffuse reflectance spectroscopy (DRS) is an effective method for hematite identification and quantification with lower detection limits. In this study, we synthesized a set of samples with well-determined concentrations to explore the exact detectable range of hematite and propose the most effective transfer function between the DRS proxy and hematite concentration. In addition, natural sediments from Inland Asia and the Western Pacific Ocean were used to further test the feasibility of the new transfer function. Results show that the lowest DRS detection limit for hematite could reach ~0.00078%, but is affected by the natural matrix. We also find that the second derivative of the Kubelka–Munk (K–M) function is monotonically correlated with the hematite concentration (0.00078%–100%), but ambiguities exist for the first derivative. Therefore, the second derivative of the K–M function is highly suggested for the hematite quantification, especially when concentration exhibits a wide range of variations. This study provides important references for the application of hematite proxy and promotes the popularization and development of the DRS method.
A Red-Emitting Cu(I)–Halide Cluster Phosphor with Near-Unity Photoluminescence Efficiency for High-Power wLED Applications
Authors: Zhaxi Wenjiang , Li Miao , Wu Jing , Liu Luying , Huang Zetao , Miao Huixian , Ma Xiao , Jiang Shenlong , Zhang Qun , Huang Wei , Wu Dayu
Published: July 2022

Solid-state lighting technology, where light-emitting diodes (LEDs) are used for energy conversion from electricity to light, is considered a next-generation lighting technology. One of the significant challenges in the field is the synthesis of high-efficiency phosphors for designing phosphor-converted white LEDs under high flux operating currents. Here, we reported the synthesis, structure, and photophysical properties of a tetranuclear Cu(I)–halide cluster phosphor, [bppmCu2I2]2 (bppm = bisdiphenylphosphinemethane), for the fabrication of high-performance white LEDs. The PL investigations demonstrated that the red emission exhibits a near-unity photoluminescence quantum yield at room temperature and unusual spectral broadening with increasing temperature in the crystalline state. Considering the excellent photophysical properties, the crystalline sample of [bppmCu2I2]2 was successfully applied for the fabrication of phosphor-converted white LEDs. The prototype white LED device exhibited a continuous rise in brightness in the range of a high bias current (100–1000 mA) with CRI as high as 84 and CCT of 5828 K, implying great potential for high-quality white LEDs.
Identification of MiRNA–Disease Associations Based on Information of Multi-Module and Meta-Path
Authors: Li Zihao , Huang Xing , Shi Yakun , Zou Xiaoyong , Li Zhanchao , Dai Zong
Published: July 2022

Cumulative research reveals that microRNAs (miRNAs) are involved in many critical biological processes including cell proliferation, differentiation and apoptosis. It is of great significance to figure out the associations between miRNAs and human diseases that are the basis for finding biomarkers for diagnosis and targets for treatment. To overcome the time-consuming and labor-intensive problems faced by traditional experiments, a computational method was developed to identify potential associations between miRNAs and diseases based on the graph attention network (GAT) with different meta-path mode and support vector (SVM). Firstly, we constructed a multi-module heterogeneous network based on the meta-path and learned the latent features of different modules by GAT. Secondly, we found the average of the latent features with weight to obtain a final node representation. Finally, we characterized miRNA–disease-association pairs with the node representation and trained an SVM to recognize potential associations. Based on the five-fold cross-validation and benchmark datasets, the proposed method achieved an area under the precision–recall curve (AUPR) of 0.9379 and an area under the receiver–operating characteristic curve (AUC) of 0.9472. The results demonstrate that our method has an outstanding practical application performance and can provide a reference for the discovery of new biomarkers and therapeutic targets.
Highly Active Amino-Fullerene Derivative-Modified TiO2 for Enhancing Formaldehyde Degradation Efficiency under Solar-Light Irradiation
Authors: Fan Jingbiao , Wang Tao , Wu Bo , Wang Chunru
Published: July 2022

Formaldehyde (HCHO) is a ubiquitous indoor pollutant that seriously endangers human health. The removal of formaldehyde effectively at room temperature has always been a challenging problem. Here, a kind of amino-fullerene derivative (C60-EDA)-modified titanium dioxide (C60-EDA/TiO2) was prepared by one-step hydrothermal method, which could degrade the formaldehyde under solar light irradiation at room temperature with high efficiency and stability. Importantly, the introduction of C60-EDA not only increases the adsorption of the free formaldehyde molecules but also improves the utilization of sunlight and suppresses photoelectron-hole recombination. The experimental results indicated that the C60-EDA/TiO2 nanoparticles exhibit much higher formaldehyde removal efficiency than carboxyl-fullerene-modified TiO2, pristine TiO2 nanoparticles, and almost all other reported formaldehyde catalysts especially in the aspect of the quality of formaldehyde that is treated by catalyst with unit mass (mHCHO/mcatalyst = 40.85 mg/g), and the removal efficiency has kept more than 96% after 12 cycles. Finally, a potential formaldehyde degradation pathway was deduced based on the situ diffuse reflectance infrared Fourier transform spectrometry (DRIFTS) and reaction intermediates. This work provides some indications into the design and fabrication of the catalysts with excellent catalytic performances for HCHO removal at room temperature.
Influences of Plasma Plume Length on Structural, Optical and Dye Degradation Properties of Citrate-Stabilized Silver Nanoparticles Synthesized by Plasma-Assisted Reduction
Authors: Acharya Tirtha Raj , Lee Geon Joon , Choi Eun Ha
Published: July 2022

Citrate-capped silver nanoparticles (Ag@Cit NPs) were synthesized by a simple plasma-assisted reduction method. Homogenous colloidal Ag@Cit NPs solutions were produced by treating a AgNO3-trisodium citrate-deionized water with an atmospheric-pressure argon plasma jet. The plasma-synthesized Ag@Cit NPs exhibited quasi-spherical shape with an average particle diameter of about 5.9−7.5 nm, and their absorption spectra showed surface plasmon resonance peaks at approximately 406 nm. The amount of Ag@Cit NPs increased in a plasma exposure duration-dependent manner. Plasma synthesis of Ag@Cit NPs was more effective in the 8.5 cm plume jet than in the shorter and longer plume jets. A larger amount of Ag@Cit NPs were produced from the 8.5 cm plume jet with a higher pH and a larger number of aqua electrons, indicating that the synergetic effect between plasma electrons and citrate plays an important role in the plasma synthesis of Ag@Cit NPs. Plasma-assisted citrate reduction facilitates the synthesis of Ag@Cit NPs, and citrate-capped nanoparticles are stabilized in an aqueous solution due to their repulsive force. Next, we demonstrated that plasma-synthesized Ag@Cit NPs exhibited a significant degradation of methylene blue dye.
(Bio)Sensing Strategies Based on Ionic Liquid-Functionalized Carbon Nanocomposites for Pharmaceuticals: Towards Greener Electrochemical Tools
Authors: Torrinha Álvaro , Oliveira Thiago M. B. F. , Ribeiro Francisco W. P. , de Lima-Neto Pedro , Correia Adriana N. , Morais Simone
Published: July 2022

The interaction of carbon-based nanomaterials and ionic liquids (ILs) has been thoroughly exploited for diverse electroanalytical solutions since the first report in 2003. This combination, either through covalent or non-covalent functionalization, takes advantage of the unique characteristics inherent to each material, resulting in synergistic effects that are conferred to the electrochemical (bio)sensing system. From one side, carbon nanomaterials offer miniaturization capacity with enhanced electron transfer rates at a reduced cost, whereas from the other side, ILs contribute as ecological dispersing media for the nanostructures, improving conductivity and biocompatibility. The present review focuses on the use of this interesting type of nanocomposites for the development of (bio)sensors specifically for pharmaceutical detection, with emphasis on the analytical (bio)sensing features. The literature search displayed the conjugation of more than 20 different ILs and several carbon nanomaterials (MWCNT, SWCNT, graphene, carbon nanofibers, fullerene, and carbon quantum dots, among others) that were applied for a large set (about 60) of pharmaceutical compounds. This great variability causes a straightforward comparison between sensors to be a challenging task. Undoubtedly, electrochemical sensors based on the conjugation of carbon nanomaterials with ILs can potentially be established as sustainable analytical tools and viable alternatives to more traditional methods, especially concerning in situ environmental analysis.
Antifouling Systems Based on Copper and Silver Nanoparticles Supported on Silica, Titania, and Silica/Titania Mixed Oxides
Authors: Calabrese Carla , Parola Valeria La , Cappello Simone , Visco Annamaria , Scolaro Cristina , Liotta Leonarda Francesca
Published: July 2022

Silica, titania, and mixed silica–titania powders have been used as supports for loading 5 wt% Cu, 5 wt% Ag, and 2.5 wt% Cu-2.5 wt% Ag with the aim of providing a series of nanomaterials with antifouling properties. All the solids were easily prepared by the wetness-impregnation method from commercially available chemical precursors. The resulting materials were characterized by several techniques such as X-ray diffraction analysis, X-ray photoelectron spectroscopy, N2 physisorption, and temperature-programmed reduction measurements. Four selected Cu and Ag SiO2- and TiO2-supported powders were tested as fillers for the preparation of marine antifouling coatings and complex viscosity measurements. Titania-based coatings showed better adhesion than silica-based coatings and the commercial topcoat. The addition of fillers enhances the resin viscosity, suggesting better workability of titania-based coatings than silica-based ones. The ecotoxicological performance of the powders was evaluated by Microtox luminescence tests, using the marine luminescent bacterium Vibrio fisheri. Further investigations of the microbiological activity of such materials were carried out focusing on the bacterial growth of Pseudoalteromonas sp., Alteromonas sp., and Pseudomonas sp. through measurements of optical density at 600 nm (OD600nm).
Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
Authors: Digiacomo Luca , Quagliarini Erica , Marmiroli Benedetta , Sartori Barbara , Perini Giordano , Papi Massimiliano , Capriotti Anna Laura , Montone Carmela Maria , Cerrato Andrea , Caracciolo Giulio , Pozzi Daniela
Published: July 2022

Magnetic levitation (MagLev) has recently emerged as a powerful method to develop diagnostic technologies based on the exploitation of the nanoparticle (NP)–protein corona. However, experimental procedures improving the robustness, reproducibility, and accuracy of this technology are largely unexplored. To contribute to filling this gap, here, we investigated the effect of total flow rate (TFR) and flow rate ratio (FRR) on the MagLev patterns of microfluidic-generated graphene oxide (GO)–protein complexes using bulk mixing of GO and human plasma (HP) as a reference. Levitating and precipitating fractions of GO-HP samples were characterized in terms of atomic force microscopy (AFM), bicinchoninic acid assay (BCA), and one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (1D SDS-PAGE), and nanoliquid chromatography–tandem mass spectrometry (nano-LC-MS/MS). We identified combinations of TFR and FRR (e.g., TFR = 35 μL/min and FRR (GO:HP) = 9:1 or TFR = 3.5 μL/min and FRR (GO:HP) = 19:1), leading to MagLev patterns dominated by levitating and precipitating fractions with bulk-like features. Since a typical MagLev experiment for disease detection is based on a sequence of optimization, exploration, and validation steps, this implies that the optimization (e.g., searching for optimal NP:HP ratios) and exploration (e.g., searching for MagLev signatures) steps can be performed using samples generated by bulk mixing. When these steps are completed, the validation step, which involves using human specimens that are often available in limited amounts, can be made by highly reproducible microfluidic mixing without any ex novo optimization process. The relevance of developing diagnostic technologies based on MagLev of coronated nanomaterials is also discussed.
Phase Shift Design in RIS Empowered Wireless Networks: From Optimization to AI-Based Methods
Authors: Li Zongze , Wang Shuai , Lin Qingfeng , Li Yang , Wen Miaowen , Wu Yik-Chung , Poor H. Vincent
Published: July 2022

Reconfigurable intelligent surfaces (RISs) offer the potential to customize the radio propagation environment for wireless networks. To fully exploit the advantages of RISs in wireless systems, the phases of the reflecting elements must be jointly designed with conventional communication resources, such as beamformers, the transmit power, and computation time. However, due to the unique constraints on the phase shifts and the massive numbers of reflecting units and users in large-scale networks, the resulting optimization problems are challenging to solve. This paper provides a review of the current optimization methods and artificial-intelligence-based methods for handling the constraints imposed by RISs and compares them in terms of the solution quality and computational complexity. Future challenges in phase-shift optimization involving RISs are also described, and potential solutions are discussed.
Pharmacological and Non-Pharmacological Agents versus Bovine Colostrum Supplementation for the Management of Bone Health Using an Osteoporosis-Induced Rat Model
Authors: Kydonaki Eirini K. , Freitas Laura , Reguengo Henrique , Simón Carlos Raposo , Bastos Ana R. , Fernandes Emanuel M. , Canadas Raphaël F. , Oliveira Joaquim M. , Correlo Vitor M. , Reis Rui L. , Vliora Maria , Gkiata Paraskevi , Koutedakis Yiannis , Ntina Georgia , Pinto Rui , Carrillo Andres E. , Marques Franklim , Amorim Tânia
Published: July 2022

Osteoporosis is defined by loss of bone mass and deteriorated bone microarchitecture. The present study compared the effects of available pharmacological and non-pharmacological agents for osteoporosis [alendronate (ALE) and concomitant supplementation of vitamin D (VD) and calcium (Ca)] with the effects of bovine colostrum (BC) supplementation in ovariectomized (OVX) and orchidectomized (ORX) rats. Seven-month-old rats were randomly allocated to: (1) placebo-control, (2) ALE group (7.5 μg/kg of body weight/day/5 times per week), (3) VD/Ca group (VD: 35 μg/kg of body weight/day/5 times per week; Ca: 13 mg/kg of body weight/day/3 times per week), and (4) BC supplementation (OVX: 1.5 g/day/5 times per week; ORX: 2 g/day/5 times per week). Following four months of supplementation, bone microarchitecture, strength and bone markers were evaluated. ALE group demonstrated significantly higher Ct.OV, Ct.BMC, Tb.Th, Tb.OV and Tb.BMC and significantly lower Ct.Pr, Tb.Pr, Tb.Sp, Ct.BMD and Tb.BMD, compared to placebo (p < 0.05). BC presented significantly higher Ct.Pr, Ct.BMD, Tb.Pr, Tb.Sp, and Tb.BMD and significantly lower Ct.OV, Ct.BMC, Tb.Th, Tb.OV and Tb.BMC compared to ALE in OVX rats (p < 0.05). OVX rats receiving BC experienced a significant increase in serum ALP and OC levels post-supplementation (p < 0.05). BC supplementation may induce positive effects on bone metabolism by stimulating bone formation, but appear not to be as effective as ALE.
The Dietary Inflammatory Index and Early COPD: Results from the National Health and Nutrition Examination Survey
Authors: Chen Chen , Yang Ting , Wang Chen
Published: July 2022

We examined 3962 people aged 20 to 49 years who had information on spirometry testing and underwent a 24 h dietary recall interview from the 2007–2012 National Health and Nutrition Examination Survey (NHANES) and used multivariable logistic regression to evaluate associations between Dietary Inflammatory Index (DII, a pro-inflammatory diet) and early COPD and lung function. The overall prevalence of early COPD was 5.05%. Higher DII was associated with increased odds of early COPD (quartile 4 vs. 1, the OR = 1.657, 95% CI = 1.100–2.496, p = 0.0156). In a full-adjusted model, each unit of increase in DII score was associated with a 90.3% increase in the risk of early COPD. Higher DII is significantly associated with lower FEV1 and FVC among individuals with early COPD, each unit increment in the DII was significantly associated with 0.43 L–0.58 L decrements in FEV1 (β = –0.43, 95% CI = −0.74, −0.12) and FVC (β = −0.58, 95% CI = −1.01, −0.16). These findings demonstrate that higher consumption of a pro-inflammatory diet may contribute to an increased risk of early COPD and lower lung function, and further support dietary interventions as part of a healthy lifestyle in order to preserve lung function and prevent or improve COPD.
Effect of Carotenoids on Paraoxonase-1 Activity and Gene Expression
Authors: Otocka-Kmiecik Aneta
Published: July 2022

Paraoxonase 1 (PON1) is an antioxidant enzyme attached to HDL with an anti-atherogenic potential. It protects LDL and HDL from lipid peroxidation. The enzyme is sensitive to various modulating factors, such as genetic polymorphisms as well as pharmacological, dietary (including carotenoids), and lifestyle interventions. Carotenoids are nutritional pigments with antioxidant activity. The aim of this review was to gather evidence on their effect on the modulation of PON1 activity and gene expression. Carotenoids administered as naturally occurring nutritional mixtures may present a synergistic beneficial effect on PON1 status. The effect of carotenoids on the enzyme depends on age, ethnicity, gender, diet, and PON1 genetic variation. Carotenoids, especially astaxanthin, β-carotene, and lycopene, increase PON1 activity. This effect may be explained by their ability to quench singlet oxygen and scavenge free radicals. β-carotene and lycopene were additionally shown to upregulate PON1 gene expression. The putative mechanisms of such regulation involve PON1 CpG-rich region methylation, Ca(2+)/calmodulin-dependent kinase II (CaMKKII) pathway induction, and upregulation via steroid regulatory element-binding protein-2 (SREBP-2). More detailed and extensive research on the mechanisms of PON1 modulation by carotenoids may lead to the development of new targeted therapies for cardiovascular diseases.
Randomised Controlled Trial of Fish Oil Supplementation on Responsiveness to Resistance Exercise Training in Sarcopenic Older Women
Authors: da Cruz Alves Natália Maira , Pfrimer Karina , Santos Priscila Carvalho , de Freitas Ellen Cristini , Neves Thiago , Pessini Rodrigo Antônio , Junqueira-Franco Márcia Varella Morandi , Nogueira-Barbosa Marcello H. , Greig Carolyn Anne , Ferriolli Eduardo
Published: July 2022

This study aims to investigate the effects of fish oil supplementation on the muscle adaptive response to resistance exercise training, physical performance and serum levels of inflammatory cytokines in sarcopenic older women. A randomised, double-blind, placebo-controlled trial is performed with thirty-four sarcopenic women (2010 European Consensus of Sarcopenia), aged ≥ 65 years. The participants are allocated into the following two groups: Exercise and Fish Oil (EFO) and Exercise and Placebo (EP). Both groups undertook a resistance exercise programme over 14 weeks. All participants are instructed to ingest 4 g/day of food supplements; the EP group received sunflower oil capsules, and the EFO group, fish oil capsules. The cross-sectional area (CSA) of the quadriceps muscle is calculated using magnetic resonance imaging (MRI). The strength of the lower limbs is measured using isokinetic dynamometry. Both groups show improvements in CSA and strength after the intervention. Changes in EFO are significantly greater compared with EP for muscle strength (peak torque, 19.46 Nm and 5.74 Nm, respectively, p < 0.001). CSA increased after the intervention in both groups (EFO; 6.11% and EP; 2.91%), although there is no significant difference between the groups (p = 0.23). There are no significant intra-group, inter-group or time differences in any of the cytokines measured. The use of fish oil supplementation potentiates the neuromuscular response to the anabolic stimulus from training, increasing muscle strength and physical performance in sarcopenic older women.
The Role of Immune Checkpoint Blockade in Acute Myeloid Leukemia
Authors: Silva Margarida , Martins Diana , Mendes Fernando
Published: July 2022

Immune checkpoint inhibition (ICI) has emerged as a therapeutic option for acute myeloid leukemia (AML) for patients that suffer from relapsed or high-risk disease, or patients ineligible for standard therapy. We aimed to study ICI as monotherapy and/or combined therapy (with chemotherapy (QT), for AML patients. The PRISMA statement was used. The literature used comprised clinical trials, randomized controlled trials, and systematic reviews published within the last 7 years. The blockade of CTLA-4 presented a 42% of complete remission within AML. Nivolumab in high-risk AML showed a median recurrence-free survival (RFS) of 8.48 months. The same drug on relapsed hematologic malignancies after allogenic transplantation shows a 1-year OS of 56%. The use of prophylaxis post allogenic transplantation cyclophosphamide (PTCy), following checkpoint inhibition, demonstrated different baseline disease and transplantation characteristics when compared to no-PCTy patients, being 32% and 10%, respectively. CTLA-4 blockage was a worthy therapeutic approach in relapsed hematologic malignancies, presenting long-lasting responses. The approach to AML and myelodysplastic syndrome patients with ICI before allogenic hematopoietic stem cell transplantation and the use of a graft-versus-host disease prophylaxis have shown improvement in the transplantation outcomes, and therefore AML treatment.
Correction: Martins et al. Cannabis-Based Products for the Treatment of Skin Inflammatory Diseases: A Timely Review. Pharmaceuticals 2022, 15, 210
Authors: Martins Ana M. , Gomes Ana L. , Boas Inês Vilas , Marto Joana , Ribeiro Helena M.
Published: July 2022

The authors would like to make the following corrections about the published paper [...]
Biomaterial-Based Therapeutic Strategies for Obesity and Its Comorbidities
Authors: Li Jing , Duan Hongli , Liu Yan , Wang Lu , Zhou Xing
Published: July 2022

Obesity is a global public health issue that results in many health complications or comorbidities, including type 2 diabetes mellitus, cardiovascular disease, and fatty liver. Pharmacotherapy alone or combined with either lifestyle alteration or surgery represents the main modality to combat obesity and its complications. However, most anti-obesity drugs are limited by their bioavailability, target specificity, and potential toxic effects. Only a handful of drugs, including orlistat, liraglutide, and semaglutide, are currently approved for clinical obesity treatment. Thus, there is an urgent need for alternative treatment strategies. Based on the new revelation of the pathogenesis of obesity and the efforts toward the multi-disciplinary integration of materials, chemistry, biotechnology, and pharmacy, some emerging obesity treatment strategies are gradually entering the field of preclinical and clinical research. Herein, by analyzing the current situation and challenges of various new obesity treatment strategies such as small-molecule drugs, natural drugs, and biotechnology drugs, the advanced functions and prospects of biomaterials in obesity-targeted delivery, as well as their biological activities and applications in obesity treatment, are systematically summarized. Finally, based on the systematic analysis of biomaterial-based obesity therapeutic strategies, the future prospects and challenges in this field are proposed.
Fluorescent PLGA Nanocarriers for Pulmonary Administration: Influence of the Surface Charge
Authors: Areny-Balagueró Aina , Mekseriwattana Wid , Camprubí-Rimblas Marta , Stephany Andrea , Roldan Ariana , Solé-Porta Anna , Artigas Antonio , Closa Daniel , Roig Anna
Published: July 2022

Nearly four million yearly deaths can be attributed to respiratory diseases, prompting a huge worldwide health emergency. Additionally, the COVID-19 pandemic’s death toll has surpassed six million, significantly increasing respiratory disease morbidity and mortality rates. Despite recent advances, it is still challenging for many drugs to be homogeneously distributed throughout the lungs, and specifically to reach the lower respiratory tract with an accurate sustained dose and minimal systemic side effects. Engineered nanocarriers can provide increased therapeutic efficacy while lessening potential biochemical adverse reactions. Poly(lactic-co-glycolic acid) (PLGA), a biodegradable polymer, has attracted significant interest as an inhalable drug delivery system. However, the influence of the nanocarrier surface charge and its intratracheal instillation has not been addressed so far. In this study, we fabricated red fluorescent PLGA nanocapsules (NCs)—Cy5/PLGA—with either positive (Cy5/PLGA+) or negative surface charge (Cy5/PLGA-). We report here on their excellent colloidal stability in culture and biological media, and after cryo-storage. Their lack of cytotoxicity in two relevant lung cell types, even for concentrations as high as 10 mg/mL, is also reported. More importantly, differences in the NCs’ cell uptake rates and internalization capacity were identified. The uptake of the anionic system was faster and in much higher amounts—10-fold and 2.5-fold in macrophages and epithelial alveolar cells, respectively. The in vivo study demonstrated that anionic PLGA NCs were retained in all lung lobules after 1 h of being intratracheally instilled, and were found to accumulate in lung macrophages after 24 h, making those nanocarriers especially suitable as a pulmonary immunomodulatory delivery system with a marked translational character.
Therapeutic Cancer Vaccines—Antigen Discovery and Adjuvant Delivery Platforms
Authors: Alarcon Neftali Ortega , Jaramillo Maddy , Mansour Heidi M. , Sun Bo
Published: July 2022

For decades, vaccines have played a significant role in protecting public and personal health against infectious diseases and proved their great potential in battling cancers as well. This review focused on the current progress of therapeutic subunit vaccines for cancer immunotherapy. Antigens and adjuvants are key components of vaccine formulations. We summarized several classes of tumor antigens and bioinformatic approaches of identification of tumor neoantigens. Pattern recognition receptor (PRR)-targeting adjuvants and their targeted delivery platforms have been extensively discussed. In addition, we emphasized the interplay between multiple adjuvants and their combined delivery for cancer immunotherapy.
Yttrium Oxide (Y2O3) as a Pulse Initiator in a Mode-Locking Erbium-Doped Fiber Laser
Authors: Zulkipli N. F. , Muhammad A. R. , Batumalay M. , Rosol A. H. A. , Altuncu A. , Durak F. E. , Ma’mun M. F. , Harun S. W.
Published: July 2022

Mode-locking is an ultra-short pulse laser generation technique. The range of pulse duration may vary from picoseconds to femtoseconds. Yttrium Oxide (Y2O3) based saturable absorber (SA) was appropriately revealed in the mode-locked method within the 1.55-micron regime. Y2O3 is perfect for strength, melting point, and chemical stability and can be used as a laminated insulator due to its properties. Moreover, Y2O3 also owns broadband service, switching speed, and engineering features. The Y2O3-PVA film was produced by combining the 50 mg Y2O3 powder into a 50 mL polyvinyl alcohol (PVA) solution and stirring it at room temperature for about 24 h. A mode-locked pulse was recorded with the integrated Y2O3-PVA SA in the erbium-doped fiber laser (EDFL) cavity, and the output spectrum optical spectrum analyzer displayed was around 1560.66 nm. In addition to the sustained mode-locked pulse, a nearly constant repetition rate of 1.01 MHz at a specific pump power begins from 175.87 mW to 228.04 mW while the pulse duration is 4.15 ps. Lastly, the mode-locked pulse had been evaluated, which showed the peak power started from 4.94 kW to 6.07 kW.
Synthesis and Characterization of Copolymers and Nanocomposites from Limonene, Styrene and Organomodified-Clay Using Ultrasonic Assisted Method
Authors: Derdar Hodhaifa , Mitchell Geoffey Robert , Chaibedraa Sarra , Mahendra Vidhura Subash , Cherifi Zakaria , Bachari Khaldoun , Chebout Redouane , Touahra Fouzia , Meghabar Rachid , Belbachir Mohammed
Published: July 2022

In the present work, we report a simple synthesis method for preparation of copolymers and nanocomposites from limonene and styrene using clay as a catalyst. The copolymerization reaction is carried out by using a proton exchanged clay as a catalyst called Mag-H+. The effect of temperature, reaction time and amount of catalyst were studied, and the obtained copolymer structure (lim-co-sty) is characterized by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (1H-NMR) and differential scanning calorimetry (DSC). The molecular weight of the obtained copolymer is determined by gel permeation chromatography (GPC) and is about 4500 g·mol−1. The (lim-co-sty/Mag 1%, 3%, 7% and 10% by weight of clay) nanocomposites were prepared through polymer/clay mixture in solution method using ultrasonic irradiation, in the presence of Mag-CTA+ as green nano-reinforcing filler. The Mag-CTA+ is organophilic silicate clay prepared through a direct exchange process, using cetyltrimethylammonuim bromide (CTAB). The prepared lim-co-sty/Mag nanocomposites have been extensively characterized by FT-IR spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and transmission electronic microscopy (TEM). TEM analysis confirms the results obtained by XRD and clearly show that the obtained nanocomposites are partially exfoliated for the lower amount of clay (1% and 3% wt) and intercalated for higher amounts of clay (7% and 10% wt). Moreover, thermogravimetric analysis (TGA) indicated an enhancement of thermal stability of nanocomposites compared with the pure copolymer.
Investigation of Low-Cost FDM-Printed Polymers for Elevated-Temperature Applications
Authors: Storck Jan Lukas , Ehrmann Guido , Güth Uwe , Uthoff Jana , Homburg Sarah Vanessa , Blachowicz Tomasz , Ehrmann Andrea
Published: July 2022

While fused deposition modeling (FDM) and other relatively inexpensive 3D printing methods are nowadays used in many applications, the possible areas of using FDM-printed objects are still limited due to mechanical and thermal constraints. Applications for space, e.g., for microsatellites, are restricted by the usually insufficient heat resistance of the typical FDM printing materials. Printing high-temperature polymers, on the other hand, necessitates special FDM printers, which are not always available. Here, we show investigations of common polymers, processible on low-cost FDM printers, under elevated temperatures of up to 160 °C for single treatments. The polymers with the highest dimensional stability and mechanical properties after different temperature treatments were periodically heat-treated between -40 °C and +80 °C in cycles of 90 min, similar to the temperature cycles a microsatellite in the low Earth orbit (LEO) experiences. While none of the materials under investigation fully maintains its dimensions and mechanical properties, filled poly(lactic acid) (PLA) filaments were found most suitable for applications under these thermal conditions.