bluuebunny/crossref_metadata_2025 huggingface dataset:
Drivers of Natural Variation in Water-Use Efficiency Under Fluctuating Light Are Promising Targets for Improvement in Sorghum
Authors: Pignon Charles P. , Leakey Andrew D. B. , Long Stephen P. , Kromdijk Johannes
Published: February 2021

Improving leaf intrinsic water-use efficiency (iWUE), the ratio of photosynthetic CO2 assimilation to stomatal conductance, could decrease crop freshwater consumption. iWUE has primarily been studied under steady-state light, but light in crop stands rapidly fluctuates. Leaf responses to these fluctuations substantially affect overall plant performance. Notably, photosynthesis responds faster than stomata to decreases in light intensity: this desynchronization results in substantial loss of iWUE. Traits that could improve iWUE under fluctuating light, such as faster stomatal movement to better synchronize stomata with photosynthesis, show significant natural diversity in C3 species. However, C4 crops have been less closely investigated. Additionally, while modification of photosynthetic or stomatal traits independent of one another will theoretically have a proportionate effect on iWUE, in reality these traits are inter-dependent. It is unclear how interactions between photosynthesis and stomata affect natural diversity in iWUE, and whether some traits are more tractable drivers to improve iWUE. Here, measurements of photosynthesis, stomatal conductance and iWUE under steady-state and fluctuating light, along with stomatal patterning, were obtained in 18 field-grown accessions of the C4 crop sorghum. These traits showed significant natural diversity but were highly correlated, with important implications for improvement of iWUE. Some features, such as gradual responses of photosynthesis to decreases in light, appeared promising for improvement of iWUE. Other traits showed tradeoffs that negated benefits to iWUE, e.g., accessions with faster stomatal responses to decreases in light, expected to benefit iWUE, also displayed more abrupt losses in photosynthesis, resulting in overall lower iWUE. Genetic engineering might be needed to break these natural tradeoffs and achieve optimal trait combinations, e.g., leaves with fewer, smaller stomata, more sensitive to changes in photosynthesis. Traits describing iWUE at steady-state, and the change in iWUE following decreases in light, were important contributors to overall iWUE under fluctuating light.
Faith-Based Coping Among Arabic-Speaking Refugees Seeking Mental Health Services in Berlin, Germany: An Exploratory Qualitative Study
Authors: Rayes Diana , Karnouk Carine , Churbaji Dana , Walther Lena , Bajbouj Malek
Published: February 2021

Background: The benefits of faith-based coping or using religious and spiritual beliefs as a stabilizing force for interpreting stressful or distressing events are largely unexplored among the exodus of Arabic-speaking refugee populations from Muslim-majority countries, particularly those resettled in Europe. The present study aimed to explore the manifestation of faith-based coping strategies among Arabic-speaking refugee adults seeking mental healthcare services in Berlin, Germany and explore how favorable faith-based coping strategies can be optimized from a mental health service-delivery and broader integration perspective.

Methods: A total of 17 qualitative interviews were conducted with Arabic-speaking refugee adults (six females, 11 males) seeking mental health services at the Charité Universitaetsmedizin in Berlin. Research questions aimed to solicit comprehensive perspectives from refugee adults on their mental health, with an emphasis on faith-based coping, and how this facilitated or impeded their integration into German society. Interview transcripts were translated to English from Arabic and analyzed using MAXQDA (2018) to highlight thematic patterns using a grounded theory approach.

Results: Findings were structured into four themes, including: (I) faith-based coping methods during flight, (II) changes in faith practices upon arrival, (III) faith-based coping methods to address distress during integration, and (IV) advice for German mental healthcare providers. Participants who demonstrated a stronger commitment to faith were more likely to utilize faith-based coping strategies when seeking mental health services and facing the challenges of displacement and integration. Examples of faith-based coping included prayer, supplication, reciting scripture, and seeking help from a local religious leader.

Conclusion: The findings suggest how faith and faith practices play a significant role in the mental health and integration of refugee populations in Germany and provide insight on how mental healthcare can be delivered in a culturally-sensitive manner, providing alternatives to the social, cultural, and linguistic barriers posed by the German health system. These findings are particularly relevant for mental health professionals, non-governmental organizations, and humanitarian aid agencies providing mental healthcare to Arabic-speaking populations recently resettled in Western contexts.
Health-Promoting Phytonutrients Are Higher in Grass-Fed Meat and Milk
Authors: van Vliet Stephan , Provenza Frederick D. , Kronberg Scott L.
Published: February 2021

While commission reports and nutritional guidelines raise concerns about the effects of consuming red meat on human health, the impacts of how livestock are raised and finished on consumer health are generally ignored. Meat and milk, irrespective of rearing practices, provide many essential nutrients including bioavailable protein, zinc, iron, selenium, calcium, and/or B12. Emerging data indicate that when livestock are eating a diverse array of plants on pasture, additional health-promoting phytonutrients—terpenoids, phenols, carotenoids, and anti-oxidants—become concentrated in their meat and milk. Several phytochemicals found in grass-fed meat and milk are in quantities comparable to those found in plant foods known to have anti-inflammatory, anti-carcinogenic, and cardioprotective effects. As meat and milk are often not considered as sources of phytochemicals, their presence has remained largely underappreciated in discussions of nutritional differences between feedlot-fed (grain-fed) and pasture-finished (grass-fed) meat and dairy, which have predominantly centered around the ω-3 fatty acids and conjugated linoleic acid. Grazing livestock on plant-species diverse pastures concentrates a wider variety and higher amounts of phytochemicals in meat and milk compared to grazing monoculture pastures, while phytochemicals are further reduced or absent in meat and milk of grain-fed animals. The co-evolution of plants and herbivores has led to plants/crops being more productive when grazed in accordance with agroecological principles. The increased phytochemical richness of productive vegetation has potential to improve the health of animals and upscale these nutrients to also benefit human health. Several studies have found increased anti-oxidant activity in meat and milk of grass-fed vs. grain-fed animals. Only a handful of studies have investigated the effects of grass-fed meat and dairy consumption on human health and show potential for anti-inflammatory effects and improved lipoprotein profiles. However, current knowledge does not allow for direct linking of livestock production practices to human health. Future research should systematically assess linkages between the phytochemical richness of livestock diets, the nutrient density of animal foods, and subsequent effects on human metabolic health. This is important given current societal concerns about red meat consumption and human health. Addressing this research gap will require greater collaborative efforts from the fields of agriculture and medicine.
Genetic and Morphological Diversity of Indigenous Bradyrhizobium Nodulating Soybean in Organic and Conventional Family Farming Systems
Authors: Gitonga Nicholas Mawira , Njeru Ezekiel Mugendi , Cheruiyot Richard , Maingi John M.
Published: February 2021

Organic farming systems are gaining popularity as agronomically and environmentally sound soil management strategies with potential to enhance soil microbial diversity and fertility, environmental quality and sustainable crop production. This work aimed at understanding the effect of organic and conventional farming on the diversity of soybean nodulating bradyrhizobia species. Field trapping of indigenous soybean Bradyrhizobium was done by planting promiscuous soybeans varieties SB16 and SC squire as well as non-promiscuous Gazelle in three organic and three conventional farms in Tharaka-Nithi County of Kenya. After 45 days of growth, 108 nodule isolates were obtained from the soybean nodules and placed into 13 groups based on their morphological characteristics. Genetic diversity was done by polymerase chain reaction (PCR) targeting 16S rDNA gene using universal primers P5-R and P3-F and sequencing was carried out using the same primer. High morphological and genetic diversity of the nodule isolates was observed in organic farms as opposed to conventional farms. There was little or no genetic differentiation between the nodule isolates from the different farms with the highest molecular variation (91.12%) being partitioned within populations as opposed to among populations (8.88%). All the isolates were identified as bradyrhizobia with close evolutionary ties with Bradyrhizobium japonicum and Bradyrhizobium yuanminense. Organic farming systems favor the proliferation of bradyrhizobia species and therefore a suitable environmentally friendly alternative for enhancing soybean production.
Methaemoglobinaemia Can Complicate Normothermic Machine Perfusion of Human Livers
Authors: Tingle Samuel J. , Ibrahim Ibrahim , Thompson Emily R. , Bates Lucy , Sivaharan Ashwin , Bury Yvonne , Figuereido Rodrigo , Wilson Colin
Published: February 2021

Background: Although liver normothermic machine perfusion is increasingly used clinically, there are few reports of complications or adverse events. Many centers perform liver NMP to viability test suboptimal grafts, often for prolonged periods. In addition, several researchers are investigating NMP as a drug delivery platform, which usually necessitates prolonged perfusion of otherwise non-viable liver grafts. We describe two instances of methaemoglobinaemia during NMP of suboptimal livers.

Methods: The NMP of eight human livers rejected for transplantation is described. Methaemoglobinaeima developed in two; one perfused using generic Medtronic™ perfusion equipment and one using the OrganOx Metra®.

Results: The first liver (53 years DBD) developed methaemoglobinaemia (metHb = 2.4%) after 13 h of NMP, increasing to metHb = 19% at 16 h. Another liver (45 years DBD) developed methaemoglobinaemia at 25 h (metHb = 2.8%), which increased to metHb = 28.2% at 38 h. Development of methaemoglobinaemia was associated with large reductions in oxygen delivery and oxygen extraction. Both livers were steatotic and showed several suboptimal features on viability testing. Delivery of methylene blue failed to reverse the methaemoglobinaemia. Compared to a matched cohort of steatotic organs, livers which developed methaemoglobinaemia showed significantly higher levels of hemolysis at 12 h (prior to development of methaemoglobinaemia).

Conclusions: Methaemglobinaemia is a complication of NMP of suboptimal liver grafts, not limited to a single machine or perfusion protocol. It can occur within 13 h (a timepoint frequently surpassed when NMP is used clinically) and renders further perfusion futile. Therefore, metHb should be monitored during NMP visually and using blood gas analysis.
Evidence of Mitochondrial Dysfunction in Bacterial Chondronecrosis With Osteomyelitis–Affected Broilers
Authors: Ferver Alison , Greene Elizabeth , Wideman Robert , Dridi Sami
Published: February 2021

A leading cause of lameness in modern broilers is bacterial chondronecrosis with osteomyelitis (BCO). While it is known that the components of BCO are bacterial infection, necrosis, and inflammation, the mechanism behind BCO etiology is not yet fully understood. In numerous species, including chicken, mitochondrial dysfunction has been shown to have a role in the pathogenicity of numerous diseases. The mitochondria is a known target for intracellular bacterial infections, similar to that of common causative agents in BCO, as well as a known regulator of cellular metabolism, stress response, and certain types of cell death. This study aimed to determine the expression profile of genes involved in mitochondrial biogenesis, dynamics, and function. RNA was isolated form the tibias from BCO-affected and healthy broilers and used to measure target gene expression via real-time qPCR. Mitochondrial biogenesis factors PGC-1α and PGC-1β were both significantly upregulated in BCO along with mitochondrial fission factors OMA1, MTFR1, MTFP1, and MFF1 as well as cellular respiration-related genes FOXO3, FOXO4, and av-UCP. Conversely, genes involved in mitochondrial function, ANT, COXIV, and COX5A showed decreased mRNA levels in BCO-affected tibia. This study is the first to provide evidence of potential mitochondrial dysfunction in BCO bone and warrants further mechanistic investigation into how this dysfunction contributes to BCO etiology.
Improved Control Scheduling Based on Learning to Prediction Mechanism for Efficient Machine Maintenance in Smart Factory
Authors: Malik Sehrish , Kim DoHyeun
Published: February 2021

The prediction mechanism is very crucial in a smart factory as they widely help in improving the product quality and customer’s experience based on learnings from past trends. The implementation of analytics tools to predict the production and consumer patterns plays a vital rule. In this paper, we put our efforts to find integrated solutions for smart factory concerns by proposing an efficient task management mechanism based on learning to scheduling in a smart factory. The learning to prediction mechanism aims to predict the machine utilization for machines involved in the smart factory, in order to efficiently use the machine resources. The prediction algorithm used is artificial neural network (ANN) and the learning to prediction algorithm used is particle swarm optimization (PSO). The proposed task management mechanism is evaluated based on multiple scenario simulations and performance analysis. The comparisons analysis shows that proposed task management system significantly improves the machine utilization rate and drastically drops the tasks instances missing rate and tasks starvation rate.
Rethinking Environmental Flows for the Yellow River Estuary by Trading Off Crop Yield and Ecological Benefits
Authors: Pang Aiping , Zhao Fen , Li Chunhui , Yi Yujun
Published: February 2021

To solve the water use conflicts between agriculture and ecosystems in arid and semiarid areas, a multi-objective trade-off analysis method was applied to determine the environmental flows (e-flows) for the Yellow River Estuary, by considering the temporal and spatial discrepancies in water allocation. The results showed that during average years, a loss of 3.7 × 108 yuan was caused with every 1 × 108 m3 of e-flows under the baseline scenario. The crop growth stages of April–July are sensitive periods for water requirements, and over 5000 yuan/ha production losses were caused by prioritizing e-flows during this time in dry years. The stages from July–October require more water by ecosystems than other stages, and the recommended e-flows during this time accounted for 57% of the e-flows during the total year. Under scenarios 1–3, which represent the short-term, medium-term and long-term scenarios, more water resources were supplied by underground water and water diversion projects; however, alleviating the water use contradiction remained difficult in dry years. During average years, e-flows between 148 and 168 × 108 m3 are recommended to meet the ecological objectives of survival, reproduction and biological integrity of species for the Yellow River Estuary. The recommended e-flows in wet years could meet higher ecological objectives but still barely achieve the targets of sediment transport and ecosystem dynamic balance. In dry years, the economic losses may be beyond the acceptance of irrigation stakeholders if more water is allocated to improve e-flows. In this case, 71 × 108 yuan would be paid to them to compensate for their losses. This study proposes an e-flow recommendation framework that is economically and ecologically optimal in areas with irreconcilable water-use contradictions.
An Immunohistochemical Study of the PTEN/AKT Pathway Involvement in Canine and Feline Mammary Tumors
Authors: Asproni Pietro , Millanta Francesca , Ressel Lorenzo , Podestà Fabio , Parisi Francesca , Vannozzi Iacopo , Poli Alessandro
Published: February 2021

Phosphatase and tensin homolog deleted on chromosome10 (PTEN), phospho-v-Akt murine thymoma viral oncogene homolog (AKT), and the Rapamycin-Insensitive Companion of mTOR (Rictor) expression was investigated by immunohistochemistry in 10 canine mammary adenomas (CMAs), 40 canine mammary carcinomas (CMCs), and 30 feline mammary carcinomas (FMCs). All the CMAs, 25 of 40 CMCs (63%) and 7 of 30 FMCs (23%), were PTEN-positive. In dogs, no CMAs and 15 of 25 CMCs (37%) expressed phospho-AKT (p-AKT), while 24 of 30 FMCs (82%) were p-AKT-positive. One of 10 CMAs (10%), 24 of 40 CMCs (60%) and 20 of 30 FMCs (67%) were Rictor-positive. In the dog, PTEN expression correlated with less aggressive tumors, absence of lymphatic invasion, and longer survival. P-AKT expression correlated with more aggressive subtype, lymphatic invasion, and poorer survival and Rictor expression with lymphatic invasion. In cats, PTEN correlated with less aggressive carcinomas, absence of lymphatic invasion, and better survival. P-AKT and Rictor expression correlated with poorer survival. PTEN expression was inversely correlated with p-AKT and Rictor in both species, while p-AKT positively correlated with Rictor expression. A strong PTEN/AKT pathway involvement in behavior worsening of CMT and FMTs is demonstrated, providing a rationale for further studies of this pathway in veterinary oncology.
Comorbidity Pattern Analysis for Predicting Amyotrophic Lateral Sclerosis
Authors: Huang Chia-Hui , Yip Bak-Sau , Taniar David , Hwang Chi-Shin , Pai Tun-Wen
Published: February 2021

Electronic Medical Records (EMRs) can be used to create alerts for clinicians to identify patients at risk and to provide useful information for clinical decision-making support. In this study, we proposed a novel approach for predicting Amyotrophic Lateral Sclerosis (ALS) based on comorbidities and associated indicators using EMRs. The medical histories of ALS patients were analyzed and compared with those of subjects without ALS, and the associated comorbidities were selected as features for constructing the machine learning and prediction model. We proposed a novel weighted Jaccard index (WJI) that incorporates four different machine learning techniques to construct prediction systems. Alternative prediction models were constructed based on two different levels of comorbidity: single disease codes and clustered disease codes. With an accuracy of 83.7%, sensitivity of 78.8%, specificity of 85.7%, and area under the receiver operating characteristic curve (AUC) value of 0.907 for the single disease code level, the proposed WJI outperformed the traditional Jaccard index (JI) and scoring methods. Incorporating the proposed WJI into EMRs enabled the construction of a prediction system for analyzing the risk of suffering a specific disease based on comorbidity combinatorial patterns, which could provide a fast, low-cost, and noninvasive evaluation approach for early diagnosis of a specific disease.
Enhanced Reinforcement Learning Method Combining One-Hot Encoding-Based Vectors for CNN-Based Alternative High-Level Decisions
Authors: Gu Bonwoo , Sung Yunsick
Published: February 2021

Gomoku is a two-player board game that originated in ancient China. There are various cases of developing Gomoku using artificial intelligence, such as a genetic algorithm and a tree search algorithm. Alpha-Gomoku, Gomoku AI built with Alpha-Go’s algorithm, defines all possible situations in the Gomoku board using Monte-Carlo tree search (MCTS), and minimizes the probability of learning other correct answers in the duplicated Gomoku board situation. However, in the tree search algorithm, the accuracy drops, because the classification criteria are manually set. In this paper, we propose an improved reinforcement learning-based high-level decision approach using convolutional neural networks (CNN). The proposed algorithm expresses each state as One-Hot Encoding based vectors and determines the state of the Gomoku board by combining the similar state of One-Hot Encoding based vectors. Thus, in a case where a stone that is determined by CNN has already been placed or cannot be placed, we suggest a method for selecting an alternative. We verify the proposed method of Gomoku AI in GuPyEngine, a Python-based 3D simulation platform.
Automated Classification of Evidence of Respect in the Communication through Twitter
Authors: Fiok Krzysztof , Karwowski Waldemar , Gutierrez Edgar , Liciaga Tameika , Belmonte Alessandro , Capobianco Rocco
Published: February 2021

Volcanoes of hate and disrespect erupt in societies often not without fatal consequences. To address this negative phenomenon scientists struggled to understand and analyze its roots and language expressions described as hate speech. As a result, it is now possible to automatically detect and counter hate speech in textual data spreading rapidly, for example, in social media. However, recently another approach to tackling the roots of disrespect was proposed, it is based on the concept of promoting positive behavior instead of only penalizing hate and disrespect. In our study, we followed this approach and discovered that it is hard to find any textual data sets or studies discussing automatic detection regarding respectful behaviors and their textual expressions. Therefore, we decided to contribute probably one of the first human-annotated data sets which allows for supervised training of text analysis methods for automatic detection of respectful messages. By choosing a data set of tweets which already possessed sentiment annotations we were also able to discuss the correlation of sentiment and respect. Finally, we provide a comparison of recent machine and deep learning text analysis methods and their performance which allowed us to demonstrate that automatic detection of respectful messages in social media is feasible.
A Repeatable and Reproducible Digital Method to Quantify the Cement Excess and Enamel Loss after Debonding Lingual Multibracket Appliance Therapy
Authors: Belanche Monterde Alba , Albaladejo Martínez Alberto , Alvarado Lorenzo Alfonso , Curto Adrián , Alonso Pérez-Barquero Jorge , Guinot-Barona Clara , Zubizarreta-Macho Álvaro
Published: February 2021

The aim of the present study is to present a repeatable, reproductible, and accurate morphometric measurement method for measuring and quantifying the area and volume of cement that remains after fixed lingual multibracket appliance debonding, enamel loss after fixed lingual multibracket appliance debonding, and the volume of cement used to adhere fixed lingual multibracket appliances. Ten conventional lingual brackets were cemented in 10 extracted teeth embedded into an epoxy resin model simulating a dental arch. This model was scanned before and after bonding the lingual brackets, after debonding, and after polishing the surfaces. We also performed a Micro-Computed Tomography scan of the lingual brackets used. Afterward, the standard tessellation language (STL) digital file was aligned, each tooth was segmented individually, and the file was re-aligned using engineer morphometry software. Inter-operator and intra-operator comparative analyses were performed using the ANOVA test, and the repeatability and reproducibility of the morphometric measurement technique were analyzed using Gage R&R statistical analysis. Repeatability showed 0.07% and 0.16% variability associated with the area and volume measures, respectively, while reproducibility showed 0.00% variability associated with the area and volume measures, respectively. In conclusion, the morphometric measurement technique is a repeatable, reproductible, and accurate morphometric measurement method for quantifying the area and volume of cement that remains after fixed lingual multibracket appliance debonding, enamel loss after fixed lingual multibracket appliance debonding, and the volume of cement used to adhere fixed lingual multibracket appliances.
Abnormal Strain Induced by Heavy Rainfall on Borehole Strainmeters Observed in Taiwan
Authors: Chen Chih-Yen , Hu Jyr-Ching , Liu Chi-Ching , Chiu Chun-Ying
Published: February 2021

We found some obvious abnormal strain induced by heavy rainfall from borehole strainmeters deployed in Western Taiwan. The strain induced by rainfall can be divided into two parts, one is the quick response for extra loads of rainwater on the ground, and another one is the slow response for rainwater infiltrating into the strata. The quick and slow rainfall responses of areal strain data are analyzed using the technique of recursive digital filtering. Moreover, the rainfall impact functions of the studied stations are calculated using deconvolution. We found, in most cases, the response strain will reach the maximum in half an hour after heavy rainfall, and then show an exponential decay, it might persist more than 200 h depending on the hydrogeological condition around the station. Whereas the river flowing beside the station will help accelerating the runoff dispersion and reducing rainfall decay time in the hill or mountain region. We also compare the results after calibration in term of isotropic and vertical coupling individually. We found that the response strains are smaller in vertical coupling rather than isotropic coupling. The effects of debris avalanches caused by intensive rainfall in the mountain areas can be viewed as two types of rock deformation: generated only under the influence of rainfall and generated by the increased load in the river channels due to rainfall-induced landslides or debris flow. When the cumulative rainfall exceeds a certain threshold, the strain response curves show a noticeable anomaly likely due to the effects of the debris flow events in places prone to landslides.
Simplified Plastic Hinge Model for Reinforced Concrete Beam–Column Joints with Eccentric Beams
Authors: Hwang Hyeon-Jong , Kim Chang-Soo
Published: February 2021

In nonlinear analysis for performance-based design of reinforced concrete moment frames, a plastic hinge spring element is predominantly used in order to simply and accurately describe the inelastic behavior of beam–column joints, including strength degradation. Although current design codes and guidelines provide various beam–column joint models, the focus is on concentric beam–column joints. Therefore, more studies are required for eccentric beam–column joints, which are also common in practice. In the present study, to consider the effect of beam eccentricity on the behavior of beam–column joints, a simplified plastic hinge model was proposed using the effective joint width of current design codes. The proposed model was compared to the cyclic loading test results of beam–column joints with/without beam eccentricity. The comparison showed that the simplified plastic hinge model with the effective joint width of NZS 3101-2006 or Eurocode 8 is considered acceptable for design purpose.
Coupled Thermomechanical Responses of Zirconium Alloy System Claddings under Neutron Irradiation
Authors: Zhao Hui , Yang Chong , Guo Dongxu , Wu Lu , Mao Jianjun , Pan Rongjian , Qin Jiantao , Shi Baodong
Published: February 2021

Zirconium (Zr) alloy is a promising fuel cladding material used widely in nuclear reactors. Usually, it is in service for a long time under the effects of neutron radiation with high temperature and high pressure, which results in thermomechanical coupling behavior during the service process. Focusing on the UO2/Zr fuel elements, the macroscopic thermomechanical coupling responses of pure Zr, Zr-Sn, and Zr-Nb binary system alloys, as well as Zr-Sn-Nb ternary system alloy as cladding materials, were studied under neutron irradiation. As a heat source, the thermal conductivity and thermal expansion coefficient models of the UO2 core were established, and an irradiation growth model of a pure Zr and Zr alloy multisystem was built. Based on the user material subroutine (UMAT) with ABAQUS, the current theoretical model was implemented into the finite element framework, and the consequent thermomechanical coupling behavior under irradiation was calculated. The distribution of temperature, the stress field of the fuel cladding, and their evolution over time were analyzed. It was found that the stress and displacement of the cladding were sensitive to alloying elements due to irradiated growth.
Combination Constraints of Multiple Fields for Implicit Modeling of Ore Bodies
Authors: Zhong De-Yun , Wang Li-Guan , Wang Jin-Miao
Published: February 2021

In this paper, we introduce combination constraints for modeling ore bodies based on multiple implicit fields interpolation. The basic idea of the method is to define a multi-labeled implicit function that combines different sub-implicit fields by the combination operations, including intersection, union and difference operators. The contribution of this paper resides in the application of combination of more general implicit fields with combination rules for the implicit modeling of ore bodies, such that the geologist can construct constraints honoring geological relationships more flexibly. To improve the efficiency of implicit surface reconstruction, a pruning strategy is used to avoid unnecessary calculations based on the hierarchical bounding box of the operation tree. Different RBF-based methods are utilized to study the implicit modeling cases of ore bodies. The experimental results of several datasets show that the combination constraints are useful to reconstruct implicit surfaces for ore bodies with mineralization rules involving multiple fields.
Attenuation of DC-Link Pulsation of a Four-Wire Inverter during Phase Unbalanced Current Operation
Authors: Zieliński Dariusz , Fatyga Karol
Published: February 2021

This paper proposes a control algorithm for a hybrid power electronic AC/DC converter for prosumer applications operating under deep phase current asymmetry. The proposed system allows independent control of active and reactive power for each phase of the power converter without current pulsation on the DC link connected to an energy store. The system and its algorithm are based on a three-phase converter in four-wire topology (AC/DC 3p-4w) with two dual-active bridge (DC/DC) converters, interfaced with a supercapacitor and an energy storage. The control algorithm tests were carried out in a Hardware in the Loop environment. Obtained results indicate that operation with deep unbalances and powers of opposite signs in individual phases leads to current oscillations in the DC link. This phenomenon significantly limits energy storage utilization due to safety and durability reasons. The proposed algorithm significantly reduces the level of pulsation in the DC link which increases safety and reduces strain on lithium-ion storage technology, enabling their application in four-wire converter applications.
Predictors of Anxiety-Induced Sleep Disturbance among in-School Adolescents in Ghana: Evidence from the 2012 Global School-Based Health Survey
Authors: Ahinkorah Bright Opoku , Aboagye Richard Gyan , Arthur-Holmes Francis , Seidu Abdul-Aziz , Frimpong James Boadu , Budu Eugene , Amoako Bernard Mensah , Hagan John Elvis
Published: February 2021

(1) Background: Psychological problems of adolescents have become a global health and safety concern. Empirical evidence has shown that adolescents experience diverse mental health conditions (e.g., anxiety, depression, and emotional disorders). However, research on anxiety-induced sleep disturbance among in-school adolescents has received less attention, particularly in low- and middle-income countries. This study’s central focus was to examine factors associated with t anxiety-induced sleep disturbance among in-school adolescents in Ghana. (2) Methods: Analysis was performed using the 2012 Global School-based Health Survey (GSHS). A sample of 1342 in-school adolescents was included in the analysis. The outcome variable was anxiety-induced sleep disturbance reported during the past 12 months. Frequencies, percentages, chi-square, and multivariable logistic regression analyses were conducted. Results from the multivariable logistic regression analysis were presented as crude and adjusted odds ratios at 95% confidence intervals (CIs) and with a statistical significance declared at p < 0.05. (3) Results: Adolescents who went hungry were more likely to report anxiety-induced sleep disturbance compared to their counterparts who did not report hunger (aOR = 1.68, CI = 1.10, 2.57). The odds of anxiety-induced sleep disturbance were higher among adolescents who felt lonely compared to those that never felt lonely (aOR = 2.82, CI = 1.98, 4.01). Adolescents who had sustained injury were more likely to have anxiety-induced sleep disturbance (aOR = 1.49, CI = 1.03, 2.14) compared to those who had no injury. Compared to adolescents who never had suicidal ideations, those who reported experiencing suicidal ideations had higher odds of anxiety-induced sleep disturbance (aOR = 1.68, CI = 1.05, 2.71). (4) Conclusions: Anxiety-induced sleep disturbance among in-school adolescents were significantly influenced by the psychosocial determinants such as hunger, loneliness, injury, and suicidal ideation in this study. The findings can help design appropriate interventions through effective strategies (e.g., early school-based screening, cognitive-behavioral therapy, face-face counseling services) to reduce psychosocial problems among in-school adolescents in Ghana.
Validation of the 8th Edition American Joint Commission on Cancer (AJCC) Gallbladder Cancer Staging System: Prognostic Discrimination and Identification of Key Predictive Factors
Authors: Giannis Dimitrios , Cerullo Marcelo , Moris Dimitrios , Shah Kevin N. , Herbert Garth , Zani Sabino , Blazer Dan G. , Allen Peter J. , Lidsky Michael E.
Published: February 2021

The scope of our study was to compare the predictive ability of American Joint Committee on Cancer (AJCC) 7th and 8th edition in gallbladder carcinoma (GBC) patients, investigate the effect of AJCC 8th nodal status on the survival, and identify risk factors associated with the survival after N reclassification using the National Cancer Database (NCDB) in the period 2005–2015. The cohort consisted of 7743 patients diagnosed with GBC; 202 patients met the criteria for reclassification and were denoted as stage ≥III by AJCC 7th and 8th edition criteria. Overall survival concordance indices were similar for patients when classified by AJCC 8th (OS c-index: 0.665) versus AJCC 7th edition (OS c-index: 0.663). Relative mortality was higher within strata of T1, T2, and T3 patients with N2 compared with N1 stage (T1 HR: 2.258, p < 0.001; T2 HR: 1.607, p < 0.001; Τ3 HR: 1.306, p < 0.001). The risk of death was higher in T1–T3 patients with Nx compared with N1 stage (T1 HR: 1.281, p = 0.043, T2 HR: 2.221, p < 0.001, T3 HR: 2.194, p < 0.001). In patients with AJCC 8th edition stage ≥IIIB GBC and an available grade, univariate analysis showed that higher stage, Charlson–Deyo score ≥ 2, higher tumor grade, and unknown nodal status were associated with an increased risk of death, while year of diagnosis after 2013, academic center, chemotherapy. and radiation therapy were associated with decreased risk of death. Chemotherapy and radiation therapy were associated with decreased risk of death in patients with T3–T4 and T2–T4 GBC, respectively. In conclusion, the updated AJCC 8th GBC staging system was comparable to the 7th edition, with the recently implemented changes in N classification assessment failing to improve the prognostic performance of the staging system. Further prospective studies are needed to validate the T2 stage subclassification as well as to clarify the association, if any is actually present, between advanced N staging and increased risk of death in patients of the same T stage.
Extracellular miRNAs as Predictive Biomarkers for Glypican-3-Derived Peptide Vaccine Therapy Response in Ovarian Clear Cell Carcinoma
Authors: Ukai Mayu , Yokoi Akira , Yoshida Kosuke , Suzuki Shiro , Shibata Kiyosumi , Kikkawa Fumitaka , Nakatsura Tetsuya , Kajiyama Hiroaki
Published: February 2021

Ovarian clear cell carcinoma (OCCC) has been treated with surgery and chemotherapy; however, the prognosis remains poor because of chemoresistance. Therefore, immunotherapies are attracting attention, including the GPC3 peptide vaccine, which improves overall survival. However, the response rate is limited and there are no sufficient predictive biomarkers that can identify responders before treatment. Our purpose was to identify circulating serum miRNAs as predictive biomarkers for response to GPC3 peptide vaccine. Eighty-four patients in a phase II trial of a GPC3 peptide vaccine were enrolled and miRNA sequencing was performed on their serum samples. Candidate miRNAs were selected from a group of 14 patients for whom treatment was responsive and validated in an independent group of 10 patients for whom treatment was responsive. Three markedly upregulated miRNAs, miR-375-3p, miR-193a-5p, and miR-1228-5p, were identified, and the combination of those miRNAs demonstrated high value in the prediction of the response. The origin of these miRNAs was assessed by referring to OCCC tissue miRNA profiles, and they were not identified as cancer tissue-related miRNAs. Functional annotation analysis suggested that they were associated with interferon-related pathways. The miRNAs identified herein have great potential to allow the realization of liquid biopsy for predicting the immunotherapy response and precision medicine.
PD-L1, TMB, MSI, and Other Predictors of Response to Immune Checkpoint Inhibitors in Biliary Tract Cancer
Authors: Rizzo Alessandro , Ricci Angela Dalia , Brandi Giovanni
Published: February 2021

Biliary tract cancer (BTC) represents the second most frequently diagnosed primary liver cancer worldwide following hepatocellular carcinoma, and the overall survival of patients with unresectable disease remains poor. In recent years, the advent of immune checkpoint inhibitors (ICIs) has revolutionized the therapeutic landscape of several malignancies with these agents, which have also been explored in advanced BTC, as monotherapy or in combination with other anticancer agents. However, clinical trials evaluating ICIs in BTC have shown conflicting results, and the clinical benefit provided by immunotherapy seems limited to a small subgroup of BTC patients. Thus, the identification of reliable predictors of the response to immunotherapy represents a significant challenge in this setting. This review provides an overview of the available evidence on the biomarkers predictive of the response to ICIs in patients with advanced BTC, especially focusing on programmed death-ligand 1 (PD-L1), tumor mutational burden (TMB), microsatellite instability (MSI), and other emerging biomarkers.
Type 2 Innate Lymphoid Cells Protect against Colorectal Cancer Progression and Predict Improved Patient Survival
Authors: Huang Qiutong , Jacquelot Nicolas , Preaudet Adele , Hediyeh-zadeh Soroor , Souza-Fonseca-Guimaraes Fernando , McKenzie Andrew N. J. , Hansbro Philip M. , Davis Melissa J. , Mielke Lisa A. , Putoczki Tracy L. , Belz Gabrielle T.
Published: February 2021

Chronic inflammation of the gastrointestinal (GI) tract contributes to colorectal cancer (CRC) progression. While the role of adaptive T cells in CRC is now well established, the role of innate immune cells, specifically innate lymphoid cells (ILCs), is not well understood. To define the role of ILCs in CRC we employed complementary heterotopic and chemically-induced CRC mouse models. We discovered that ILCs were abundant in CRC tumours and contributed to anti-tumour immunity. We focused on ILC2 and showed that ILC2-deficient mice developed a higher tumour burden compared with littermate wild-type controls. We generated an ILC2 gene signature and using machine learning models revealed that CRC patients with a high intratumor ILC2 gene signature had a favourable clinical prognosis. Collectively, our results highlight a critical role for ILC2 in CRC, suggesting a potential new avenue to improve clinical outcomes through ILC2-agonist based therapeutic approaches.
89Zr-Labeled Domain II-Specific scFv-Fc ImmunoPET Probe for Imaging Epidermal Growth Factor Receptor In Vivo
Authors: Alizadeh Elahe , Behlol Ayaz Ahmed Khan , Raja Solomon Viswas , Gaja Vijay , Bernhard Wendy , Makhlouf Amal , Gonzalez Carolina , Barreto Kris , Casaco Angel , Geyer C. Ronald , Fonge Humphrey
Published: February 2021

Epidermal growth factor receptor I (EGFR) is overexpressed in many cancers. The extracellular domain of EGFR has four binding epitopes (domains I- IV). All clinically approved anti-EGFR antibodies bind to domain III. Imaging agents that bind to domains other than domain III of EGFR are needed for accurate quantification of EGFR, patient selection for anti-EGFR therapeutics and monitoring of response to therapies. We recently developed a domain II-specific antibody fragment 8709. In this study, we have evaluated the in vitro and in vivo properties of 89Zr-8709-scFv-Fc (105 kDa). We conjugated 8709-scFv-Fc with the deferoxamine (DFO) chelator and radiolabeled the DFO-8970-scFv with 89Zr. We evaluated the binding of 89Zr-DFO-8709-scFv-Fc in EGFR positive and negative cell lines DLD-1, MDA-MB-231 and MDA-MB-435, respectively, and in mouse xenograft models. Simultaneously, we have compared the binding of 89Zr-8709-scFv-Fc with 111In-nimotuzumab, a domain III anti-EGFR antibody. DFO-8709-scFv-Fc displayed similar cell binding specificity as 8709-scFv-Fc. Saturation cell binding assay and immunoreactive fraction showed that radiolabeling did not alter the binding of 8709-scFv-Fc. Biodistribution and microPET showed good uptake of 89Zr-8709-scFv-Fc in xenografts after 120 h post injection (p.i). and was domain-specific to EGFR domain II. 89Zr-8709-scFv-Fc did not compete for binding in vitro and in vivo with a known domain III binder nimotuzumab. The results show that 89Zr-8709-scFv-Fc is specific to domain II of EGFR making it favorable for quantification of EGFR in vivo, hence, patient selection and monitoring of response to treatment with anti-EGFR antibodies.
Oxidation of Organic Compounds with Peroxides Catalyzed by Polynuclear Metal Compounds
Authors: Shul’pin Georgiy B. , Shul’pina Lidia S.
Published: February 2021

The review describes articles that provide data on the synthesis and study of the properties of catalysts for the oxidation of alkanes, olefins, and alcohols. These catalysts are polynuclear complexes of iron, copper, osmium, nickel, manganese, cobalt, vanadium. Such complexes for example are: [Fe2(HPTB)(m-OH)(NO3)2](NO3)2·CH3OH·2H2O, where HPTB-¼N,N,N0,N0-tetrakis(2-benzimidazolylmethyl)-2-hydroxo-1,3-diaminopropane; complex [(PhSiO1,5)6]2[CuO]4[NaO0.5]4[dppmO2]2, where dppm-1,1-bis(diphenylphosphino)methane; (2,3-η-1,4-diphenylbut-2-en-1,4-dione)undecacarbonyl triangulotriosmium; phenylsilsesquioxane [(PhSiO1.5)10(CoO)5(NaOH)]; bi- and tri-nuclear oxidovanadium(V) complexes [{VO(OEt)(EtOH)}2(L2)] and [{VO(OMe)(H2O)}3(L3)]·2H2O (L2 = bis(2-hydroxybenzylidene)terephthalohydrazide and L3 = tris(2-hydroxybenzylidene)benzene-1,3,5-tricarbohydrazide); [Mn2L2O3][PF6]2 (L = 1,4,7-trimethyl-1,4,7-triazacyclononane). For comparison, articles are introduced describing catalysts for the oxidation of alkanes and alcohols with peroxides, which are simple metal salts or mononuclear metal complexes. In many cases, polynuclear complexes exhibit higher activity compared to mononuclear complexes and exhibit increased regioselectivity, for example, in the oxidation of linear alkanes. The review contains a description of some of the mechanisms of catalytic reactions. Additionally presented are articles comparing the rates of oxidation of solvents and substrates under oxidizing conditions for various catalyst structures, which allows researchers to conclude about the nature of the oxidizing species. This review is focused on recent works, as well as review articles and own original studies of the authors.
Extra-Heavy Oil Aquathermolysis Using Nickel-Based Catalyst: Some Aspects of In-Situ Transformation of Catalyst Precursor
Authors: Vakhin Alexey V. , Aliev Firdavs A. , Mukhamatdinov Irek I. , Sitnov Sergey A. , Kudryashov Sergey I. , Afanasiev Igor S. , Petrashov Oleg V. , Nurgaliev Danis K.
Published: February 2021

In the present work, we studied the catalytic performance of an oil-soluble nickel-based catalyst during aquathermolysis of oil-saturated crushed cores from Boca de Jaruco extra-heavy oil field. The decomposition of nickel tallate and some aspects of in-situ transformation of the given catalyst precursor under the steam injection conditions were investigated in a high-pressure batch reactor using XRD and SEM analysis methods. The changes in physical and chemical properties of core extracts after the catalytic aquathermolysis process with various duration were studied using gas chromatography for analyzing gas products, SARA analysis, GC-MS of saturated and aromatic fractions, FT-IR spectrometer, elemental analysis, and matrix-activated laser desorption/ionization (MALDI). The results showed that nickel tallate in the presence of oil-saturated crushed core under the injection of steam at 300 °C transforms mainly into nonstoichiometric forms of nickel sulfide. According to the SEM images, the size of nickel sulfide particles was in the range of 80–100 nm. The behavior of main catalytic aquathermolysis gas products such as CH4, CO2, H2S, and H2 depending on the duration of the process was analyzed. The catalytic upgrading at 300 °C provided decrease in the content of resins and asphaltenes, and increase in saturated hydrocarbon content. Moreover, the content of low-molecular alkanes, which were not detected before the catalytic aquathermolysis process, dramatically increased in saturates fraction after catalytic aquathermolysis reactions. In addition, the aromatics hydrocarbons saturated with high molecular weight polycyclic aromatic compounds—isomers of benzo(a)fluorine, which were initially concentrated in resins and asphaltenes. Nickel sulfide showed a good performance in desulfurization of high-molecular components of extra-heavy oil. The cracking of the weak C–S bonds, which mainly concentrated in resins and asphaltenes, ring-opening reactions, detachment of alkyl substitutes from asphaltenes and inhibition of polymerization reactions in the presence of catalytic complex reduced the average molecular mass of resins (from 871.7 to 523.3 a.m.u.) and asphaltenes (from 1572.7 to 1072.3 a.m.u.). Thus, nickel tallate is a promising catalyst to promote the in-situ upgrading of extra-heavy oil during steam injection techniques.