bluuebunny/crossref_metadata_2025 huggingface dataset:
Recognition of Geothermal Surface Manifestations: A Comparison of Machine Learning and Deep Learning
Authors: Xiong Yongzhu , Zhu Mingyong , Li Yongyi , Huang Kekun , Chen Yankui , Liao Jingqing
Published: April 2022

Geothermal surface manifestations (GSMs) are direct clues towards hydrothermal activities of a geothermal system in the subsurface and significant indications for geothermal resource exploration. It is essential to recognize various GSMs for potential geothermal energy exploration. However, there is a lack of work to fulfill this task using deep learning (DL), which has achieved unprecedented successes in computer vision and image interpretation. This study aims to explore the feasibility of using a DL model to fulfill the recognition of GSMs with photographs. A new image dataset was created for the GSM recognition by preprocessing and visual interpretation with expert knowledge and a high-quality check after downloading images from the Internet. The dataset consists of seven GSM types, i.e., warm spring, hot spring, geyser, fumarole, mud pot, hydrothermal alteration, crater lake, and one type of none GSM, including 500 images of different photographs for each type. The recognition results of the GoogLeNet model were compared with those of three machine learning (ML) algorithms, i.e., Support Vector Machine (SVM), Decision Tree (DT), and K-Nearest Neighbor (KNN), by using the assessment metrics of overall accuracy (OA), overall F1 score (OF), and computational time (CT) for training and testing the models via cross-validation. The results show that the retrained GoogLeNet model using transfer learning has significant advantages of accuracies and performances over the three ML classifiers, with the highest OA, the biggest OF, and the fastest CT for both the validation and test. Correspondingly, the three selected ML classifiers perform poorly for this task due to their low OA, small OF, and long CT. This suggests that transfer learning with a pretrained network be a feasible method to fulfill the recognition of the GSMs. Hopefully, this study provides a reference paradigm to help promote further research on the application of state-of-the-art DL in the geothermics domain.
Effects of Steam Injection on the Permissible Hydrogen Content and Gaseous Emissions in a Micro Gas Turbine Supplied by a Mixture of CH4 and H2: A CFD Analysis
Authors: Reale Fabrizio
Published: April 2022

The use of hydrogen in small scale gas turbines is currently limited by several issues. Blending hydrogen with methane or other gaseous fuels can be considered a low medium-term viable solution, with the goal of reducing greenhouse gas emissions. In fact, only small amounts can be mixed with methane in premixed combustors, due to the risk of flashback. The aim of this article is to investigate the injection of small quantities of steam as a method of increasing the maximum permissible hydrogen content in a mixture with methane. The proposed approach involves introducing the steam directly into the combustion chamber into the main fuel feeding system of a Turbec T100. The study is carried out by means of CFD analysis of the combustion process. A thermodynamic analysis of the energy system is used to determine boundary conditions. The combustion chamber is discretized using a three-dimensional mesh consisting of 4.7 million nodes and the RANS RSM model is used to simulate the effects of turbulence. The results show that the addition of steam may triple the permissible percentage of hydrogen in the mixture for the considered MGT, passing from 10% to over 30% by volume, also leading to a reduction in NOx emissions without a significant variation in CO emissions.
Fluid-Structure Interaction Analysis of a Competitive Car during Brake-in-Turn Manoeuvre
Authors: Broniszewski Jakub , Piechna Janusz Ryszard
Published: April 2022

The relationship between the presented work and energy conservation is direct and indirect. Most of the literature related to energy-saving focuses on reducing the aerodynamic drag of cars, which typically leads to the appearance of vehicle motion instabilities at high speeds. Typically, this instability is compensated for by moving aerodynamic body components activated above a certain speed and left in that position until the vehicle speed drops. This change in vehicle configuration results in a significant increase in drag at high velocities. The presented study shows a fully coupled approach to fluid–structure interaction analyses of a car during a high-speed braking-in-turn manoeuvre. The results show how the aerodynamic configuration of a vehicle affects its dynamic behaviour. In this work, we used a novel approach, combining Computational Fluid Dynamics (CFD) analysis with the Multibody Dynamic System. The utilisation of an overset technique allows for car movement in the computational domain. Adding Moving Reference Frame (MRF) to this motion removes all restrictions regarding car trajectory and allows for velocity changes over time. We performed a comparative analysis for two aerodynamic configurations. In the first one, a stationary rear airfoil was in a base position parallel to a trunk generating low drag. No action of the driver was assumed. In the second scenario, brake activation initiates the rotation of the rear airfoil reaching in 0.1 s final position corresponding to maximum aerodynamic downforce generation. Also, no action of the driver was assumed. In the second scenario, the airfoil was moving from the base position up to the point when the whole system approached its maximum downforce. To determine this position, we ran a separated quasi-steady analysis in which the airfoil was rotating slowly to avoid transient effects. The obtained results show the importance of the downforce and load balance on car stability during break-in-turn manoeuvres. They also confirm that the proposed methodology of combining two independent solvers to analyse fluid–structure phenomena is efficient and robust. We captured the aerodynamic details caused by the car’s unsteady movement.
Key Techniques for Rapid Jacking and Laying of Pipelines: A Case Study on ‘Jingshihan’ Gas Pipelines in China
Authors: Xu Tianshuo , Wang Le , Zhang Peng , Zhou Yuheng , Liu Kaixin , Feng Xin , Qi Yongchun , Zeng Cong
Published: April 2022

With the rapid growth of the oil and gas storage, transportation, and pipeline industries, it is necessary to improve the construction process of oil and gas pipelines. By combining the technical advantages of horizontal directional drilling and pipe jacking construction, the direct pipe laying method is suitable for pipeline crossing in different strata in the oil and gas, water conservancy and hydropower, and municipal industries due to its advantages of less construction land, high speed, and reversibility. Using the rapid jacking and laying of pipelines crossing Nanjuma River in the ‘Jingshihan’ gas pipeline double line project as a case study, this paper investigates the application status of the direct pipe laying method, summarizes the project, and introduces the construction of the working well, equipment selection, guiding control technology, supporting equipment installation, and drag reduction measures, as well as analyzes the influencing factors of thrust force and trajectory deviation combined with formation information.
Two-Layer Ring Truss-Based Space Solar Power Station
Authors: Fan Guanheng , Zhang Yiqun , Ji Xiangfei , Yang Yang
Published: April 2022

A space solar power station (SSPS) has become a huge potential candidate to provide abundant and clean electrical energy for terrestrial users by collecting and converting solar power in space. In this paper, an innovative two-layer ring truss-based SSPS is proposed. It consists of the top layer concentrator-based spherical one-time reflection region, the bottom layer space radiator using symmetric or asymmetric cable networks, a ring truss for a supporting structure, a photoelectric conversion system, and transmitting antennas. The construction strategies including the triangular facets modularity of top layer concentrator, area requirement of bottom layer space radiator, two-segment optimization design of generatrix of photoelectric conversion system, and aperture derivation of transmitting antenna are carried out. Then, the performance analysis mainly including the modularization theory error calculation, energy collection and distribution, and thermal characteristics in orbit of this proposed SSPS is presented. Finally, the system parameters are estimated and summarized for a better sense of the proposed SSPS. The results indicate that 100% energy collection can be achieved for an ideal concentrator, and 80% with a modular division layer of 6 and tracking error of no more than 2°. The results demonstrate the feasibility of the proposed SSPS concept and can provide a reference for future space energy harvesting and space exploration projects.
Microbial Involvement in the Bioremediation of Total Petroleum Hydrocarbon Polluted Soils: Challenges and Perspectives
Authors: Chicca Ilaria , Becarelli Simone , Di Gregorio Simona
Published: April 2022

Nowadays, soil contamination by total petroleum hydrocarbons is still one of the most widespread forms of contamination. Intervention technologies are consolidated; however, full-scale interventions turn out to be not sustainable. Sustainability is essential not only in terms of costs, but also in terms of restoration of the soil resilience. Bioremediation has the possibility to fill the gap of sustainability with proper knowledge. Bioremediation should be optimized by the exploitation of the recent “omic” approaches to the study of hydrocarburoclastic microbiomes. To reach the goal, an extensive and deep knowledge in the study of bacterial and fungal degradative pathways, their interactions within microbiomes and of microbiomes with the soil matrix has to be gained. “Omic” approaches permits to study both the culturable and the unculturable soil microbial communities active in degradation processes, offering the instruments to identify the key organisms responsible for soil contaminant depletion and restoration of soil resilience. Tools for the investigation of both microbial communities, their degradation pathways and their interaction, will be discussed, describing the dedicated genomic and metagenomic approaches, as well as the interpretative tools of the deriving data, that are exploitable for both optimizing bio-based approaches for the treatment of total petroleum hydrocarbon contaminated soils and for the correct scaling up of the technologies at the industrial scale.
Application of Remote Sensing Data for Assessment of Bark Beetle Attacks in Pine Plantations in Kirkovo Region, the Eastern Rhodopes
Authors: Georgieva Margarita , Belilov Sevdalin , Dimitrov Stelian , Iliev Martin , Trenkin Vladislav , Mirchev Plamen , Georgiev Georgi
Published: April 2022

Intensive forest afforestation with native pine species was developed in the 1960s on degraded and deforested lands in the region of the Eastern Rhodopes (south-eastern Bulgaria). Severe damage by wet snow was registered in the coniferous forests of the Rhodopes in March 2015. In the following years, bark beetle attacks were registered on the broken and felled fresh wood. As a result, bark beetle infestation spots appeared in the pine plantations. In the period 2019–2021, damage caused by bark beetles was assessed in the region of State Forestry Kirkovo (the Eastern Rhodopes, south-eastern Bulgaria). An integrated approach using the data of the information system of the Executive Forest Agency (ISEFA), remote sensing data obtained by an “eBee SQ” unmanned aerial vehicle (UAV) equipped with a “Parrot Sequoia” multispectral camera, and subsequent terrestrial observations, was applied. ISEFA data showed that there was no serious damage caused by abiotic and biotic factors in the pine forests of SF Kirkovo until 2014. Snow damage in 2015 affected 513 ha of pine plantations, and bark beetle infestations reached up to 1316 ha in 2016. In 2019, a total of 226.87 ha of pine plantations were captured in three localities—Fotinovo, Kirkovo, and Kremen. The relative share of damage caused by bark beetles was greater in P. sylvestris plantations (15.3–23.0%), compared to damage in P. nigra (2.3%). Four different categories of normalised difference vegetation index (NDVI) were separated in bark beetle infestation spots—living trees, dead trees, grass and shrub vegetation, stones and rocks. The NDVI values in locations with living trees varied between 0.500 (spaces between tree crowns) and 0.700 (central part of the crown projection) (an average of 0.617). In the locations with dead trees, the average values of NDVI of lying trees was 0.273, and in standing trees, NDVI varied between 0.275 (central part of crown projections) and 0.424 (spaces between tree crowns). In the locations with grass and shrub vegetation, stones and rocks, the average NDVI was 0.436 and 0.329, respectively. In the field study, average defoliation of 31.2–32.3% was registered in P. sylvestris plantations, and 47.4% in P. nigra plantations. Defoliations mainly were caused by pine processionary moth (Thaumetopoea pityocampa) and fungal pathogens (Dothistroma septosporum and Lecanosticta acicola). The damage was caused by Ips acuminatus (in P. sylvestris only), and I. sexdentatus, Tomicus piniperda and T. minor (in P. sylvestris and P. nigra). Infestations by other xylophages, such as Phaenops cyanea, Rhagium inquisitor, and Pissodes spp., were also found on pine stems.
Functional, Physicochemical, Rheological, Microbiological, and Organoleptic Properties of Synbiotic Ice Cream Produced from Camel Milk Using Black Rice Powder and Lactobacillus acidophilus LA-5
Authors: Elkot Wael F. , Ateteallah Ateteallah H. , Al-Moalem Maalem H. , Shahein Magdy Ramadan , Alblihed Mohamed A. , Abdo Walied , Elmahallawy Ehab Kotb
Published: April 2022

Camel milk has become more popular among customers in recent years as a result of its therapeutic effects. In many parts of the world, it is considered one of the primary components of human nutrition. The present study aimed to develop a novel synbiotic ice cream from camel milk formulated with black rice powder (BRP) and investigate the viability of probiotic bacteria (Lactobacillus acidophilus LA-5) during the storage period (60 days). Skim milk powder was replaced by BRP at levels of 0, 25, 50, and 75%. The produced ice cream was examined for some physicochemical, rheological, microbiological, and sensorial properties. The obtained results indicated that the incorporation of BRP into ice cream blends resulted in significant increases in the overrun, viscosity, and melting resistance of ice cream samples (p < 0.05). However, the freezing point decreased with increasing the proportion of BRP in the blend. The sensory evaluation results showed that the most acceptable treatments were those formulated with 25% and could be increased to 50% of BRP with no significant differences. The incorporation of BRP improved the viability of Lactobacillus acidophilus LA-5 in ice cream samples over 60 days of storage. Collectively, a synbiotic camel milk ice cream formulated with black rice powder was produced that, in turn, enhanced the physicochemical and rheological properties of ice cream samples and produced a significant protective effect on the viability of probiotic bacteria.
A Review on the Utilization of Date Palm Fibers as Inclusion in Concrete and Mortar
Authors: Bamaga S. O.
Published: April 2022

Currently, natural fibers attract the attention of researchers and builders in the construction industry as they are eco-friendly, cost-effective, lightweight, and renewable resources. The inclusion of natural fibers in the concrete and mortar will contribute to solving the environmental problems associated with dumping or burning them and improve the properties and durability of concrete and mortar. Similar to other natural fibers, Date Palm Fibers (DPF) have been receiving more attention as construction materials. This paper presents a review on the properties of DPF and its effects on the physical, mechanical, and thermal properties of concrete and mortar as well as the processing of DPF and mix design. DPFs can be used in concrete and mortar to improve their properties. However, some of the properties could be reduced. Even though the conducted studies and investigations are promising, it is still not enough to introduce DPF concrete and mortar to the construction industry’s applications.
Fluctuating Asymmetry in Asteriscii Otoliths of Common Carp (Cyprinus carpio) Collected from Three Localities in Iraqi Rivers Linked to Environmental Factors
Authors: Jawad Laith , Mahé Kélig
Published: April 2022

Otoliths, calcified structures in the inner ears, are used to estimate fish age, and their shape is an efficient fish stock identification tool. Otoliths are thus very important for the management and assessment of commercial stocks. However, most studies have used left or right otoliths, chosen arbitrarily without evaluation of the difference between these otoliths. In this study, the asteriscii otoliths from 263 common carp (Cyprinus carpio; Linnaeus, 1758) were sampled in three Iraqi rivers to test the potential asymmetry and the geographical effect on otolith growth from three measurements (length, width and weight), and on shape from two shape indices (ellipticity and form-factor). Among all asteriscii otolith features, there was significant fluctuating asymmetry between fish length and every otolith descriptor. At one fish length, the size and/or the shape of otoliths could be different between two individuals and/or between left and right asteriscii otoliths for the same individual. Moreover, the relationship between fish length and otolith shape/growth was significantly dependent on the studied geographical area and, more especially, the environmental effects as the water temperature and pH. Finally, the relationships between fish length and otolith shape indices showed that the otolith evolves into the elliptical shape during the life of the fish. To use the otolith shape, it is essential to take into account the developmental stage of individuals to integrate the ontogenetic effect. Our results highlight the importance of verifying potential otolith asymmetry, especially for the asteriscii otoliths (lagenar otoliths) before their use in fisheries research.
Identification of Potential Peptide Marker(s) for Evaluating Pork Meat Freshness via Mass Spectrometry-Based Peptidomics during Storage under Different Temperatures
Authors: Wei Zhenqian , Dai Chen , Bassey Anthony P. , Tang Changbo , Han Yu , Wang Chong , Zhou Guanghong
Published: April 2022

This study applied peptidomics to investigate potential biomarkers for evaluating pork-meat freshness. The spoilage time points of pork meat stored at −2, 4, 10, and 25 °C were defined by evaluating meat freshness indicators (color, total viable count, pH, and total volatile basic nitrogen). Peptide MVHMASKE was identified as a potential peptide marker via multivariate analysis. Pearson correlation revealed a negative correlation between intensity of MVHMASKE and total viable count/total volatile basic nitrogen. In addition, the correlation between peptide content and the change in pork-meat freshness was verified using real-life samples, and the content of MVHMASKE showed a significant decline during storage under 4 and 25 °C, correspondingly reflecting the change of pork meat from fresh to spoiled. This study provides favorable evidence to evaluate pork-meat freshness by monitoring the change of peptide MVHMASKE in content based on mass spectrometry-based peptidomics.
Quality and Pro-Healthy Properties of Belgian Witbier-Style Beers Relative to the Cultivar of Winter Wheat and Raw Materials Used
Authors: Belcar Justyna , Buczek Jan , Kapusta Ireneusz , Gorzelany Józef
Published: April 2022

Unmalted wheat grain and barley malt are the basic materials used in the production of Belgian wheat beers known as Witbier. A change in the ingredients defined in the recipe, by which part of the unmalted wheat is replaced with wheat malt, can positively affect the quality of the beverage produced. The purpose of the study was to brew Witbier-style beers made from four cultivars of winter wheat, with a 50% share of unmalted wheat and barley malt as well as Witbier-style beers made from four wheat cultivars, where 25% of unmalted wheat was replaced with wheat malt. Physicochemical and sensory analyzes showed mild differences in the quality of the beer products, more specifically higher alcohol content (by 11.33%) were found in beers made without the addition of wheat malt, while higher sensory attractiveness and 17.13% higher total polyphenol content were identified in beers enhanced with wheat malt. Phenolic compounds were identified using UPLC-PDA-MS/MS. The highest flavanol content, including kaempferol 3-O-rhamnoside-7-O-pentoside, was found in beers produced using wheat grains of the ‘Elixer’ cultivar, whether or not wheat malt was added; the values were 1.31 mg/L in E50 beer, and 1.39 mg/L in E25 beer. The same beer samples with the highest antioxidant and antiradical activity were found (in E25 beer, 2.35 mmol TE/L, and in E50 beer, 2.12 mmol Fe2+/L). The present findings show that the investigated wheat cultivars may be used in beer production, whereas replacing part of unmalted wheat with wheat malt can improve the sensory profile of the beer produced.
Longitudinal Verification of Post-Nuclear Accident Food Regulations in Japan Focusing on Wild Vegetables
Authors: Osanai Minoru , Noro Tomuhiro , Kimura Shonosuke , Kudo Kohsei , Hosokawa Shota , Tsushima Megumi , Tsuchiya Ryoko , Iwaoka Kazuki , Yamaguchi Ichiro , Saito Yoko
Published: April 2022

Focusing on the importance of wild vegetables for local residents, this study aims to validate the effects of food regulations under the current criteria (e.g., 100 Bq/kg for general foods) established approximately a year after the Fukushima Dai-ichi Nuclear Power Plant accident. Over 2,500,000 monitoring tests were performed under the criteria until fiscal year (FY) 2020. We estimated changes in internal exposure dose using test results. The effective dose was estimated using the radioactive concentration randomly sampled from the results, food intake, and dose conversion factor. As a new attempt, dose estimation reflecting the intake of wild vegetables that may have irreplaceable value for local residents was conducted. The median, 95th, and 99th percentile of the estimated dose without reflecting the wild vegetables’ intake were 0.0485, 0.183, and 10.6 mSv/year, respectively, in the estimation with all test results (no regulation) and 0.0431, 0.0786, and 0.236 mSv/year, respectively, in the estimation with results within the standard limits (regulated) in FY2012. These doses decreased with time. Although estimated doses with or without the reflection of wild vegetables’ intake were similar, estimation that is more plausible is possible, particularly for a high percentile, by reflecting the wild vegetables’ intake. Radiation doses (regulated) were significantly less than 1 mSv/year in different FYs. In Japan, food regulation measures benefit food safety.
A Comprehensive Survey of Phenolic Constituents Reported in Monofloral Honeys around the Globe
Authors: Lawag Ivan Lozada , Lim Lee-Yong , Joshi Ranee , Hammer Katherine A. , Locher Cornelia
Published: April 2022

The aim of this review is to provide a comprehensive overview of the large variety of phenolic compounds that have to date been identified in a wide range of monofloral honeys found globally. The collated information is structured along several themes, including the botanical family and genus of the monofloral honeys for which phenolic constituents have been reported, the chemical classes the phenolic compounds can be attributed to, and the analytical method employed in compound determination as well as countries with a particular research focus on phenolic honey constituents. This review covers 130 research papers that detail the phenolic constituents of a total of 556 monofloral honeys. Based on the findings of this review, it can be concluded that most of these honeys belong to the Myrtaceae and Fabaceae families and that Robinia (Robinia pseudoacacia, Fabaceae), Manuka (Leptospermum scoparium, Myrtaceae), and Chestnut (Castanea sp., Fagaceae) honeys are to date the most studied honeys for phenolic compound determination. China, Italy, and Turkey are the major honey phenolic research hubs. To date, 161 individual phenolic compounds belonging to five major compound groups have been reported, with caffeic acid, gallic acid, ferulic acid and quercetin being the most widely reported among them. HPLC with photodiode array detection appears to be the most popular method for chemical structure identification.
Relandscaping the Gut Microbiota with a Whole Food: Dose–Response Effects to Common Bean
Authors: Lutsiv Tymofiy , McGinley John N. , Neil-McDonald Elizabeth S. , Weir Tiffany L. , Foster Michelle T. , Thompson Henry J.
Published: April 2022

Underconsumption of dietary fiber and the milieu of chemicals with which it is associated is a health concern linked to the increasing global burden of chronic diseases. The benefits of fiber are partially attributed to modulation of the gut microbiota, whose composition and function depend on the amount and quality of microbiota-accessible substrates in the diet. However, not all types of fiber are equally accessible to the gut microbiota. Phaseolus vulgaris L., or common bean, is a food type rich in fiber as well as other prebiotics posing a great potential to positively impact diet-microbiota-host interactions. To elucidate the magnitude of bean’s effects on the gut microbiota, increasing doses of common bean were administered in macronutrient-matched diet formulations. The microbial communities in the ceca of female and male mice were evaluated via 16S rRNA gene sequencing. As the bean dose increased, the Bacillota:Bacteroidota ratio (formerly referred to as the Firmicutes:Bacteroidetes ratio) was reduced and α-diversity decreased, whereas the community composition was distinctly different between the diet groups according to β-diversity. These effects were more pronounced in female mice compared to male mice. Compositional analyses identified a dose-responsive bean-induced shift in microbial composition. With an increasing bean dose, Rikenellaceae, Bacteroides, and RF39, which are associated with health benefits, were enhanced. More taxa, however, were suppressed, among which were Allobaculum, Oscillospira, Dorea, and Ruminococcus, which are predominantly associated with chronic disease risk. Investigation of the origins of the dose dependent and biological sex differences in response to common bean consumption may provide insights into bean-gut microbiota-host interactions important to developing food-based precision approaches to chronic disease prevention and control.
Quality Multiverse of Beef and Pork Meat in a Single Score
Authors: Rajic Sara , Simunovic Stefan , Djordjevic Vesna , Raseta Mladen , Tomasevic Igor , Djekic Ilija
Published: April 2022

The rationale behind this review is the potential of developing a single score tool for meat quality evaluation based on visual and sensorial assessments of fresh meat. Based on the known sensory wheel concept, the first step was to create quality wheels capturing most common intrinsic and extrinsic quality cues of pork and beef outlined in the latest scientific papers. This resulted in identifying meat color, sensory characteristics and fat content as the most important intrinsic quality cues of fresh beef and pork. Furthermore, the highest number of studies showed the importance of price, certification logos and brand for beef quality evaluation. According to recent articles, price, breed, animal welfare and a veterinary certificate are the most important extrinsic attributes for pork consumers. The second step was to develop a single-score tool named the “Meat quality index”. It has been developed in line with published approaches of different total quality index concepts used in the food sector, providing insights into its application in the meat sector. As a result, this review proposes a unique approach in using quality index application, through the consumer’s preferences aspect of fresh meat.
In Vitro Anti-Inflammatory Activity of Peptides Obtained by Tryptic Shaving of Surface Proteins of Streptococcus thermophilus LMD-9
Authors: Allouche Rania , Hafeez Zeeshan , Papier Florent , Dary-Mourot Annie , Genay Magali , Miclo Laurent
Published: April 2022

Streptococcus thermophilus, a lactic acid bacterium widely used in the dairy industry, is consumed regularly by a significant proportion of the population. Some strains show in vitro anti-inflammatory activity which is not fully understood. We hypothesized that peptides released from the surface proteins of this bacterium during digestion could be implied in this activity. Consequently, we prepared a peptide hydrolysate by shaving and hydrolysis of surface proteins using trypsin, and the origin of peptides was checked by liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis. Most of the identified peptides originated from bacterial cell surface proteins. The anti-inflammatory activity of peptide hydrolysate was investigated under inflammatory conditions in two cell models. Peptide hydrolysate significantly decreased secretion of pro-inflammatory cytokine IL-8 in lipopolysaccharide (LPS)-stimulated human colon epithelial HT-29 cells. It also reduced the production of pro-inflammatory cytokines IL-8, IL-1β and the protein expression levels of Pro-IL-1β and COX-2 in LPS-stimulated THP-1 macrophages. The results showed that peptides released from bacterial surface proteins by a pancreatic protease could therefore participate in an anti-inflammatory activity of S. thermophilus LMD-9 and could prevent low-grade inflammation.
Efficacy of Whey Protein Film Incorporated with Portuguese Green Tea (Camellia sinensis L.) Extract for the Preservation of Latin-Style Fresh Cheese
Authors: Robalo João , Lopes Maria , Cardoso Olga , Sanches Silva Ana , Ramos Fernando
Published: April 2022

Fresh cheese composition favors the growth of microorganisms and lipid oxidation, leading to a short shelf life. Whey protein concentrates can be used to produce active films in which green tea (Camellia sinensis L.) extract, rich in bioactive compounds, namely catechins, can be incorporated. Thus, the main objective of this study was to evaluate the efficacy of an edible active film, incorporated with green tea extract, to preserve goat and mixture (goat and sheep) fresh cheeses. Our results demonstrated that Portuguese green teas (antioxidant activity coefficient—AAC = 746.7) had superior antioxidant capacity to that of the evaluated Asian green tea (AAC = 650). Furthermore, green tea produced from the leaves of the new Portuguese Chá Camélia tea plantation had the highest potential to retain the antioxidant capacity (97.3%). Additionally, solid–liquid extractions led to extracts with higher antioxidant activity (AAC = 1500), but Soxhlet extractions presented higher yield (43%). Furthermore, the active film incorporated with Portuguese green tea extract exhibited a high antioxidant capacity (AAC ≈ 595.4). In addition, the active film effectively delayed the lipid oxidation of the evaluated fresh cheeses (3.2 mg MDA Eq/kg) when compared with the control (4.2 mg MDA Eq/kg). Moreover, the active films effectively inhibited the growth of microorganisms, especially E. coli (1.5 × 10 CFU/g), when compared with the blank (2.2 × 102 CFU/g). This study suggests that the new whey protein film incorporated with Portuguese green tea extract has the potential to be used to extend fresh cheese shelf life.
Evaluation of the Antimicrobial Activity of Four Plant Essential Oils against Some Food and Phytopathogens Isolated from Processed Meat Products in Egypt
Authors: Elshafie Shahenda S. , Elshafie Hazem S. , El Bayomi Rasha M. , Camele Ippolito , Morshdy Alaa Eldin M. A.
Published: April 2022

Synthetic preservatives are widely utilized by the food industry to inhibit the microbial contamination and increase food safety and shelf life. The excessive utilization of synthetic preservatives can have a negative impact on human health and the environment. There is a great interest to find out natural substances as possible food-preservatives. The consumers’ preference for food products with natural ingredients prompted food manufacturers to utilize natural-based preservatives in their production. It is worth noting that plant essential oils (EOs) among the natural-based substances have been efficiently used as antimicrobial agents against phyto- and food pathogens. The current study was conducted to evaluate the microbial contamination of three industrial meat products from five governorates in Egypt, identify the predominant bacterial and fungal isolates and determine the antimicrobial efficacy of some EOs (thyme, fennel, anise and marjoram) against the most predominant microbial isolates. A sensory test was also performed to estimate the customer preferences for specific organoleptic aspects of meat products after EOs treatment. Results showed that there is a promising antimicrobial activity of all studied EOs against some microbial isolates in a dose-dependent manner. In particular, thyme EO showed the highest significant antibacterial activity against P.fluorescence and E. coli. Whereas the marjoram EO showed the highest activity against P. aeruginosa. In addition, the sensory test revealed that the treatment with anise and marjoram EOs showed the highest acceptability by the testers and did not show significant differences on the organoleptic properties with respect to control. As overall, the obtained results of the current research are promising and proved feasibility of employing plant EOs as possible preservatives for processed meat products.
Fractional-Order PI Controller Design Based on Reference–to–Disturbance Ratio
Authors: Muresan Cristina I. , Birs Isabela R. , Copot Dana , Dulf Eva H. , Ionescu Clara M.
Published: April 2022

The presence of disturbances in practical control engineering applications is unavoidable. At the same time, they drive the closed-loop system’s response away from the desired behavior. For this reason, the attenuation of disturbance effects is a primary goal of the control loop. Fractional-order controllers have now been researched intensively in terms of improving the closed-loop results and robustness of the control system, compared to the standard integer-order controllers. In this study, a novel tuning method for fractional-order controllers is developed. The tuning is based on improving the disturbance attenuation of periodic disturbances with an estimated frequency. For this, the reference–to–disturbance ratio is used as a quantitative measure of the control system’s ability to reject disturbances. Numerical examples are included to justify the approach, quantify the advantages and demonstrate the robustness. The simulation results provide for a validation of the proposed tuning method.
Novel Formulation of Fusidic Acid Incorporated into a Myrrh-Oil-Based Nanoemulgel for the Enhancement of Skin Bacterial Infection Treatment
Authors: Almostafa Mervt M. , Elsewedy Heba S. , Shehata Tamer M. , Soliman Wafaa E.
Published: April 2022

Fusidic acid (FA) is renowned as an effective bacteriostatic agent obtained from the fungus Fusidium coccineum, used for treating various eye and skin disorders. The objective of the present study was to develop, characterize, and evaluate the antibacterial activity of a novel FA nanoemulgel for topical skin application. Primarily, various fusidic acid nanoemulsion formulations were fabricated using different concentrations of myrrh essential oil, Tween 80 as a surfactant, and Transcutol® P as a co-surfactant. A Box–Behnken design was employed to select the optimized FA nanoemulsion formulation, based on the evaluated particle size and % of in vitro release as dependent variables. The optimized formula was incorporated within a hydrogel to obtain an FA nanoemulgel (FA-NEG) preparation. The formulated FA-NEG was evaluated for its visual appearance, pH, viscosity, and spreadability, compared to its corresponding prepared fusidic acid gel. In vitro release, kinetic study, and ex vivo drug permeation were implemented, followed by formulation stability testing. The FA-NEG exhibited a smooth and homogeneous appearance, pH value (6.61), viscosity (25,265 cP), and spreadability (33.6 mm), which were all good characteristics for appropriate topical application. A total of 59.3% of FA was released from the FA-NEG after 3 h. The ex vivo skin permeability of the FA-NEG was significantly enhanced by 3.10 ± 0.13-fold, showing SSTF of 111.2 ± 4.5 µg/cm2·h when compared to other formulations under investigation (p < 0.05). No irritation was observed upon applying the FA-NEG to animal skin. Eventually, it was revealed that the FA-NEG displayed improved antibacterial activity against a wide variety of bacteria when compared to its corresponding FA gel and marketed cream, indicating the prospective antibacterial effect of myrrh essential oil. In conclusion, the recommended formulation offers a promising antibacterial approach for skin infections.
Graphene Oxide-Reinforced Alginate Hydrogel for Controlled Release of Local Anesthetics: Synthesis, Characterization, and Release Studies
Authors: Luu Cuong Hung , Nguyen Giang , Le Thanh-Tuyen , Nguyen Thanh-Mai Ngoc , Giang Phan V. H. , Murugesan Mohanapriya , Mathiyalagan Ramya , Jing Lu , Janarthanan Gopinathan , Yang Deok Chun , Li Yi , Thambi Thavasyappan
Published: April 2022

In pain relief, lidocaine has gained more attention as a local anesthetic. However, there are several side effects that limit the use of local anesthetics. Therefore, it is hypothesized that a hydrogel system with facile design can be used for prolonged release of lidocaine. In this study, we developed a formulation comprises of sodium alginate (SA) and graphene oxide (GO) to prolong the release of lidocaine. The gelation was induced by physically crosslinking the alginate with Ca2+ ions. The formation of blank SA and GO-reinforced SA hydrogels was investigated with different concentration of Ca2+ ions. The controlled release of lidocaine hydrochloride (LH) on both hydrogel systems was studied in PBS solution. The GO-reinforced SA hydrogels exhibited more sustained release than SA hydrogels without GO. In vitro biocompatibility test in L929 fibroblast cells confirmed the non-toxic property of hydrogels. Furthermore, to prove the in-situ gelation and biodegradability of hydrogels the hydrogels were injected on mice model and confirmed the stable gel formation. The hydrogels implanted onto the subcutaneous tissue of hydrogels retained over one week. These results indicate that LH-loaded GO-reinforced SA hydrogel can be a potential biomaterial for controlled release of local anesthetics.
A Penalization Method for Estimating Heterogeneous Covariate Effects in Cancer Genomic Data
Authors: Luo Ziye , Zhang Yuzhao , Sun Yifan
Published: April 2022

In high-throughput profiling studies, extensive efforts have been devoted to searching for the biomarkers associated with the development and progression of complex diseases. The heterogeneity of covariate effects associated with the outcomes across subjects has been noted in the literature. In this paper, we consider a scenario where the effects of covariates change smoothly across subjects, which are ordered by a known auxiliary variable. To this end, we develop a penalization-based approach, which applies a penalization technique to simultaneously select important covariates and estimate their unique effects on the outcome variables of each subject. We demonstrate that, under the appropriate conditions, our method shows selection and estimation consistency. Additional simulations demonstrate its superiority compared to several competing methods. Furthermore, applying the proposed approach to two The Cancer Genome Atlas datasets leads to better prediction performance and higher selection stability.
Complexity and Geoheritage Importance of Granite Pseudokarst from the Belaya River Gorge (Western Caucasus)
Authors: Ruban Dmitry A.
Published: April 2022

New investigations in the Western Caucasus contribute to the understanding of granite pseudokarst (sensu lato) and megaclasts linked to river erosion. A plot on the bank of the Belaya River (Mountainous Adygeya, Western Caucasus) was selected to examine diverse and abundant pseudokarst features (small rock basins, hollows, potholes, and channels) and large clasts. Morphological analysis of these features clarifies their general characteristics and genetic interpretations. Pseudokarst features can be classified into two major categories, namely the relatively small (<1 m) and large (>1 m) features. Potholes, which are usually 1–3 m in size, are the most characteristic features occurring on two levels, i.e., on steep walls of the gorge (half-filled with river water) and on slightly inclined surfaces of a terrace-like landform (subaerial exposure). In both cases, their walls from the side of the river are broken. Apparently, these potholes were formed on the river bottom. Subsequent incision of the gorge elevated potholes and the river has eroded them from one side. Apparently, some pseudokarst features are related to macroturbulent flood flows and granite weathering. Due to its scientific uniqueness and aesthetic attractiveness, this granite pseudokarst constitutes geoheritage, which can be exploited for the purposes of geoscience research and geotourism development.
Shallow Landslides and Rockfalls Velocity Assessment at Regional Scale: A Methodology Based on a Morphometric Approach
Authors: Marinelli Antonella , Medici Camilla , Rosi Ascanio , Tofani Veronica , Bianchini Silvia , Casagli Nicola
Published: April 2022

Velocity is one of the most important parameters to evaluate the damaging potential of a mass movement, but its assessment, especially for extremely rapid landslides, is a complex task. In the literature, several models to assess mass movement velocity exist, but they usually require many detailed parameters, and therefore, they are applicable only to a single slope and not usable for regional-scale analyses. This study aims to propose a simple morphometric methodology, based on the spatialisation of the Energy Line method, to determine the velocity of shallow landslides and rockfalls at a regional scale. The proposed method requires a limited amount of input data (landslide perimeters and a digital elevation model), and its application can be carried out using GIS software and a Matlab code. The test area of this work is the Valle d’Aosta Region (Northern Italy), selected due to its peculiar geological and geomorphological setting that makes this region susceptible to the occurrence of both shallow landslides and rockfalls. Since measured velocity values for rockfalls and shallow landslides were not available, the results obtained with the proposed method have been validated through the implementation of a model in the literature, namely the Gravitational Process Path (GPP) model, for some selected landslides.
Effects of Leisure-Time Physical Activity on Cognitive Reserve Biomarkers and Leisure Motivation in the Pre-Diabetes Elderly
Authors: Kim Bo-Ram , Lim Seung-Taek
Published: April 2022

The purpose of this study was to investigate the change in cognitive reserve biomarkers of the pre-diabetic individual according to the types of leisure-time physical activity (aerobic or resistance physical activity). The research subjects (n = 184) who participated in the survey were pre-diabetic and diabetic patients who were visiting university hospitals and welfare centers. The intervention subjects (n = 36) who were elderly females with pre-diabetes volunteered to participate in the study by performing regular physical exercise (aerobic or resistance exercise). The study participants were 65 years of age or older with pre-diabetes defined by a glycated hemoglobin (HbA1c) level of (5.7–6.4)%. All research subjects performed motivation and stress questionnaire survey. All intervention subjects participated in leisure-time physical activity (LTPA) for 12 weeks. Body composition, HbA1c, and cognitive reserve biomarkers were measured at baseline, and at 6 and 12 weeks. LTPA motivation confirmed that the LTPA participants had a high level of motivation. Stress confirmed that the stress level of LTPA participants was low. Two-way within-factor ANOVA revealed significant group × time interaction for weight (p < 0.05), BMI (p < 0.01), % fat (p < 0.001), SBP (p < 0.05), HbA1c (p < 0.001), BDNF (p < 0.001), and Beta-Amyloid 1–42 (p < 0.001). In both physical activity groups, HbA1c (p < 0.001), NGF (p < 0.05), BDNF (p < 0.05), and Cathepsin B (p < 0.05) improved significantly at 12 weeks, compared to baseline and 6 weeks. In the resistance physical activity group, Beta-Amyloid 1–42 (p < 0.01) and Homocysteine (p < 0.05) significantly decreased at 12 weeks, compared to baseline and at 6 weeks. The LTPA showed high levels of integrated and identified regulation among leisure motive types, and the level of stress was found to be low. The LTPA is effective in reducing the HbA1c levels of the pre-diabetes elderly. In addition, the pre-diabetes elderly were found to have increased NGF, BDNF, and cathepsin B, and decreased Beta-Amyloid 1–42 and homocysteine. Regular leisure-time physical activity has a positive effect on cognitive reserve biomarkers through improving glycemic control by reducing weight and % fat in the pre-diabetes elderly.
Temporomandibular Disorders, Bruxism, Perceived Stress, and Coping Strategies among Medical University Students in Times of Social Isolation during Outbreak of COVID-19 Pandemic
Authors: Saczuk Klara , Lapinska Barbara , Wawrzynkiewicz Adam , Witkowska Alicja , Arbildo-Vega Heber Isac , Domarecka Monika , Lukomska-Szymanska Monika
Published: April 2022

The COVID-19 pandemic caught universities along with their students off-guard, enforcing online education. Fear of the unknown, disinformation, and isolation resulted in an increased stress level in the entire population. Medical university students are particularly endangered with high stress levels and developing TMD. Temporomandibular disorders (TMD) are of multifactorial etiology, and manifest with jaw dysfunction, masticatory muscle tension or pain, as well as headache. Though bruxism can act as an exacerbating factor for TMD, stress can also play crucial role in the onset. The study aimed to measure occurrence of TMD and bruxism symptoms in the medical student population, asses the stress level, and evaluate adopted stress-coping strategies during the COVID-19 pandemic outbreak. A survey study was performed among 1018 students at Medical University of Lodz during April 2020. A self-designed questionnaire for screening TMD and bruxism symptoms, Perceived Stress Scale (PSS-10), and Brief-COPE questionnaires were applied. TMD and bruxism symptoms were observed in the majority of subjects during social isolation. The perceived stress levels were significantly higher in those experiencing TMD and bruxism symptoms. Mostly maladaptive, emotion-focused coping strategies were chosen by study subjects experiencing high levels of stress. Choosing Self-Blaming as a coping strategy is the strongest predictor of perceived stress.