bluuebunny/crossref_metadata_2025 huggingface dataset:
Anti-CD3 Antibody Treatment Reduces Scar Formation in a Rat Model of Myocardial Infarction
Authors: Wernly Bernhard , Paar Vera , Aigner Achim , Pilz Patrick M , Podesser Bruno K , Förster Martin , Jung Christian , Pinon Hofbauer Josefina , Tockner Birgit , Wimmer Monika , Kraus Theo , Motloch Lukas J , Hackl Matthias , Hoppe Uta C , Kiss Attila , Lichtenauer Michael
Published: January 2020

Introduction: Antibody treatment with anti-thymocyte globulin (ATG) has been shown to be cardioprotective. We aimed to evaluate which single anti-T-cell epitope antibody alters chemokine expression at a level similar to ATG and identified CD3, which is a T-cell co-receptor mediating T-cell activation. Based on these results, the effects of anti-CD3 antibody treatment on angiogenesis and cardioprotection were tested in vitro and in vivo. Methods: Concentrations of IL-8 and MCP-1 in supernatants of human peripheral blood mononuclear cell (PBMC) cultures following distinct antibody treatments were evaluated by Enzyme-linked Immunosorbent Assay (ELISA). In vivo, anti-CD3 antibodies or vehicle were injected intravenously in rats subjected to acute myocardial infarction (AMI). Chemotaxis and angiogenesis were evaluated using tube and migration assays. Intracellular pathways were assessed using Western blot. Extracellular vesicles (EVs) were quantitatively evaluated using fluorescence-activated cell scanning, exoELISA, and nanoparticle tracking analysis. Also, microRNA profiles were determined by next-generation sequencing. Results: Only PBMC stimulation with anti-CD3 antibody led to IL-8 and MCP-1 changes in secretion, similar to ATG. In a rat model of AMI, systemic treatment with an anti-CD3 antibody markedly reduced infarct scar size (27.8% (Inter-quartile range; IQR 16.2–34.9) vs. 12.6% (IQR 8.3–27.2); p < 0.01). The secretomes of anti-CD3 treated PBMC neither induced cardioprotective pathways in cardiomyocytes nor pro-angiogenic mechanisms in human umbilical vein endothelial cell (HUVECs) in vitro. While EVs quantities remained unchanged, PBMC incubation with an anti-CD3 antibody led to alterations in EVs miRNA expression. Conclusion: Treatment with an anti-CD3 antibody led to decreased scar size in a rat model of AMI. Whereas cardioprotective and pro-angiogenetic pathways were unaltered by anti-CD3 treatment, qualitative changes in the EVs miRNA expression could be observed, which might be causal for the observed cardioprotective phenotype. We provide evidence that EVs are a potential cardioprotective treatment target. Our findings will also provide the basis for a more detailed analysis of putatively relevant miRNA candidates.
A-Kinase Anchoring Protein 1: Emerging Roles in Regulating Mitochondrial Form and Function in Health and Disease
Authors: Liu Yujia , Merrill Ronald A. , Strack Stefan
Published: January 2020

Best known as the powerhouse of the cell, mitochondria have many other important functions such as buffering intracellular calcium and reactive oxygen species levels, initiating apoptosis and supporting cell proliferation and survival. Mitochondria are also dynamic organelles that are constantly undergoing fission and fusion to meet specific functional needs. These processes and functions are regulated by intracellular signaling at the mitochondria. A-kinase anchoring protein 1 (AKAP1) is a scaffold protein that recruits protein kinase A (PKA), other signaling proteins, as well as RNA to the outer mitochondrial membrane. Hence, AKAP1 can be considered a mitochondrial signaling hub. In this review, we discuss what is currently known about AKAP1′s function in health and diseases. We focus on the recent literature on AKAP1′s roles in metabolic homeostasis, cancer and cardiovascular and neurodegenerative diseases. In healthy tissues, AKAP1 has been shown to be important for driving mitochondrial respiration during exercise and for mitochondrial DNA replication and quality control. Several recent in vivo studies using AKAP1 knockout mice have elucidated the role of AKAP1 in supporting cardiovascular, lung and neuronal cell survival in the stressful post-ischemic environment. In addition, we discuss the unique involvement of AKAP1 in cancer tumor growth, metastasis and resistance to chemotherapy. Collectively, the data indicate that AKAP1 promotes cell survival throug regulating mitochondrial form and function. Lastly, we discuss the potential of targeting of AKAP1 for therapy of various disorders.
On the Quality of Protein Crystals Grown under Diffusion Mass-transport Controlled Regime (I)
Authors: Gavira José A. , Otálora Fermín , González-Ramírez Luis A. , Melero Emilio , Driessche Alexander E.S. van , García-Ruíz Juan Manuel
Published: January 2020

It has been previously shown that the diffraction quality of protein crystals strongly depends on mass transport during their growth. In fact, several studies support the idea that the higher the contribution of the diffusion during mass transport, the better the diffraction quality of the crystals. In this work, we have compared the crystal quality of two model (thaumatin and insulin) and two target (HBII and HBII-III) proteins grown by two different methods to reduce/eliminate convective mass transport: crystal growth in agarose gels and crystal growth in solution under microgravity. In both cases, we used identical counterdiffusion crystallization setups and the same data collection protocols. Additionally, critical parameters such as reactor geometry, stock batches of proteins and other chemicals, temperature, and duration of the experiments were carefully monitored. The diffraction datasets have been analyzed using a principal component analysis (PCA) to determine possible trends in quality indicators. The relevant indicators show that, for the purpose of structural crystallography, there are no obvious differences between crystals grown under reduced convective flow in space and convection-free conditions in agarose gel, indicating that the key factor contributing to crystal quality is the reduced convection environment and not how this reduced convection is achieved. This means that the possible detrimental effect on crystal quality due to the incorporation of gel fibers into the protein crystals is insignificant compared to the positive impact of an optimal convection-free environment provided by gels. Moreover, our results confirm that the counterdiffusion technique optimizes protein crystal quality and validates both environments in order to deliver high quality protein crystals, although other considerations, such as protein/gel interactions, must be considered when defining the optimal crystallization setup.
Evolution of Neuroaesthetic Variables in Portrait Paintings throughout the Renaissance
Authors: Correa-Herran Ivan , Aleem Hassan , Grzywacz Norberto
Published: January 2020

To compose art, artists rely on a set of sensory evaluations performed fluently by the brain. The outcome of these evaluations, which we call neuroaesthetic variables, helps to compose art with high aesthetic value. In this study, we probed whether these variables varied across art periods despite relatively unvaried neural function. We measured several neuroaesthetic variables in portrait paintings from the Early and High Renaissance, and from Mannerism. The variables included symmetry, balance, and contrast (chiaroscuro), as well as intensity and spatial complexities measured by two forms of normalized entropy. The results showed that the degree of symmetry remained relatively constant during the Renaissance. However, the balance of portraits decayed abruptly at the end of the Early Renaissance, that is, at the closing of the 15th century. Intensity and spatial complexities, and thus entropies, of portraits also fell in such manner around the same time. Our data also showed that the decline of complexity and entropy could be attributed to the rise of chiaroscuro. With few exceptions, the values of aesthetic variables from the top of artists of the Renaissance resembled those of their peers. We conclude that neuroaesthetic variables have flexibility to change in brains of artists (and observers).
On the Information Bottleneck Problems: Models, Connections, Applications and Information Theoretic Views
Authors: Zaidi Abdellatif , Estella-Aguerri Iñaki , Shamai (Shitz) Shlomo
Published: January 2020

This tutorial paper focuses on the variants of the bottleneck problem taking an information theoretic perspective and discusses practical methods to solve it, as well as its connection to coding and learning aspects. The intimate connections of this setting to remote source-coding under logarithmic loss distortion measure, information combining, common reconstruction, the Wyner–Ahlswede–Korner problem, the efficiency of investment information, as well as, generalization, variational inference, representation learning, autoencoders, and others are highlighted. We discuss its extension to the distributed information bottleneck problem with emphasis on the Gaussian model and highlight the basic connections to the uplink Cloud Radio Access Networks (CRAN) with oblivious processing. For this model, the optimal trade-offs between relevance (i.e., information) and complexity (i.e., rates) in the discrete and vector Gaussian frameworks is determined. In the concluding outlook, some interesting problems are mentioned such as the characterization of the optimal inputs (“features”) distributions under power limitations maximizing the “relevance” for the Gaussian information bottleneck, under “complexity” constraints.
Using a System Dynamics Modelling Process to Determine the Impact of eCar, eBus and eTruck Market Penetration on Carbon Emissions in South Africa
Authors: Sooknanan Pillay Nalini , Brent Alan Colin , Musango Josephine Kaviti , van Geems Francois
Published: January 2020

The complexities that are inherent in electricity value chains are non-linear in nature and they require unconventional modelling methods, such as system dynamics. This paper provides an overview of the system dynamics method applied for obtaining an understanding of the impact of electric-bus, -car, and -truck market penetration on carbon emissions in South Africa, through the development of the electric mobility simulator (eMobiSim). Two scenarios were tested. The World Reference scenario was based on a market penetration of 22% eCars, 19% eTrucks, and 80% eBuses and the Gross Domestic Product (GDP) scenario was based on 2.38% eCars, 1.79% eTrucks, and 12% eBuses. The results indicate that the World Reference scenario is the most optimistic, with a 12.33% decrease in carbon emissions in the transport sector and an increase of 4.32% in the electricity sector. However, if the economic structure that is specific to South Africa is to be considered and the GDP scenario is run, then there would only be a 1.77% decrease of carbon emissions in the transport sector and an increase of 0.64% in the electricity sector. Although the eCar market penetration produces the highest reduction in carbon emissions, the volumes that are required are large and other factors, such as price parity and affordability in the various income deciles, would have to be considered in determining whether this volume is achievable.
Platinum and Platinum Group Metal-Free Catalysts for Anion Exchange Membrane Fuel Cells
Authors: Men Truong Van , Richard Tolchard Julian , Svendby Jørgen , Manikandan Maidhily , A. Miller Hamish , Sunde Svein , Yang Hsiharng , R. Dekel Dario , Oyarce Barnett Alejandro
Published: January 2020

The development of active hydrogen oxidation reaction (HOR) and oxygen reduction reaction (ORR) catalysts for use in anion exchange membrane fuel cells (AEMFCs), which are free from platinum group metals (PGMs), is expected to bring this technology one step closer to commercial applications. This paper reports our recent progress developing HOR Pt-free and PGM-free catalysts (Pd/CeO2 and NiCo/C, respectively), and ORR PGM-free Co3O4 for AEMFCs. The catalysts were prepared by different synthesis techniques and characterized by both physical-chemical and electrochemical methods. A hydrothermally synthesized Co3O4 + C composite ORR catalyst used in combination with Pt/C as HOR catalyst shows good H2/O2 AEMFC performance (peak power density of ~388 mW cm−2), while the same catalyst coupled with our flame spray pyrolysis synthesised Pd/CeO2 anode catalysts reaches peak power densities of ~309 mW cm−2. Changing the anode to nanostructured NiCo/C catalyst, the performance is significantly reduced. This study confirms previous conclusions, that is indeed possible to develop high performing AEMFCs free from Pt; however, the challenge to achieve completely PGM-free AEMFCs still remains.
A Comparison of the Formation Rates and Composition of Tree-Related Microhabitats in Beech-Dominated Primeval Carpathian and Hyrcanian Forests
Authors: Jahed Razieh Rafiei , Kavousi Mohammad Reza , Farashiani Mohammad Ebrahim , Sagheb-Talebi Khosro , Babanezhad Manoochehr , Courbaud Benoit , Wirtz Roland , Müller Jörg , Larrieu Laurent
Published: January 2020

Primeval forests in the temperate zone exist only as a few remnants, but theses serve as important reference areas for conservation. As key habitats, tree-related microhabitats (TreMs) are of intense interest to forest ecologists, but little is known about their natural composition and dynamics in different tree species. Beech forms a major part of the temperate forests that extend from Europe, home to European beech Fagus sylvatica L. (Fs), eastward to Iran, where Oriental beech Fagus orientalis Lipsky (Fo) is the dominant species. In this study, we compared TreMs in primeval forests of both species, using data from Fo growing in 25 inventory plots throughout the Hyrcanian forest belt in Iran and from Fs growing in a 9 ha permanent plot in the Uholka Forest of Ukraine. TreMs based on 47 types and 11 subgroups were recorded. Beech trees in the Hyrcanian forest had a higher mean diameter at breast height (dbh) than beech trees in Uholka and contained twice as many TreMs per hectare. Although the mean richness of TreMs per TreM bearing tree was similar in the two species, on the basis of the comparison single trees in two groups (n = 405 vs. 2251), the composition of the TreMs clearly differed, as the proportions of rot holes, root-buttress concavities, and crown deadwood were higher in the Hyrcanian Forest, and those of bark losses, exposed heartwood, and burrs and cankers higher in Uholka Forest. Estimates of TreMs dynamics based on dbh and using Weibull models showed a significantly faster cumulative increase of TreMs in Fo, in which saturation occurred already in trees with a dbh of 70–80 cm. By contrast, the increase in TreMs in Fs was continuous. In both species, the probability density was highest at a dbh of about 30 cm, but was twice as high in Fo. Because of limitations of our study design, the reason behind observed differences of TreM formation and composition between regions remains unclear, as it could be either result of the tree species or the environment, or their interaction. However, the observed differences were more likely the result of differences in the environment than in the two tree species. Nevertheless, our findings demonstrate that the Hyrcanian Forest, recently designated as a natural heritage site in Iran, is unique, not only as a tertiary relict or due to its endemic trees, herbs and arthropods, but also because of its TreMs, which form a distinct and rich habitat for associated taxa, including endemic saproxylic species.
From Dendrochronology to Allometry
Authors: Biondi Franco
Published: January 2020

The contribution of tree-ring analysis to other fields of scientific inquiry with overlapping interests, such as forestry and plant population biology, is often hampered by the different parameters and methods that are used for measuring growth. Here I present relatively simple graphical, numerical, and mathematical considerations aimed at bridging these fields, highlighting the value of crossdating. Lack of temporal control prevents accurate identification of factors that drive wood formation, thus crossdating becomes crucial for any type of tree growth study at inter-annual and longer time scales. In particular, exactly dated tree rings, and their measurements, are crucial contributors to the testing and betterment of allometric relationships.
Co-Existence of Inoculated Yeast and Lactic Acid Bacteria and Their Impact on the Aroma Profile and Sensory Traits of Tempranillo Red Wine
Authors: Izquierdo-Cañas Pedro Miguel , Ríos-Carrasco María , García-Romero Esteban , Mena-Morales Adela , Heras-Manso José María , Cordero-Bueso Gustavo
Published: January 2020

This study investigates the effects of simultaneous inoculation of a selected Saccharomyces cerevisiae yeast strain with two different commercial strains of wine bacteria Oenococcus oeni at the beginning of the alcoholic fermentation on the kinetics of malolactic fermentation (MLF), wine chemical composition, and organoleptic characteristics in comparison with spontaneous MLF in Tempranillo grape must from Castilla-La Mancha (Spain). Evolution of MLF was assessed by the periodic analysis of L-malic acid through the enzymatic method, and most common physiochemical parameters and sensory traits were evaluated using a standardized sensory analysis. The samples were analyzed by GC/MS in SCAN mode using a Trace GC gas chromatograph and a DSQII quadrupole mass analyzer. Co-inoculation reduced the overall fermentation time by up to 2 weeks leading to a lower increase in volatile acidity. The fermentation-derived wine volatiles profile was distinct between the co-inoculated wines and spontaneous MLF and was influenced by the selected wine bacteria used in co-inoculation. Co-inoculation allows MLF to develop under reductive conditions and results in wines with very few lactic and buttery flavors, which is related to the impact of specific compounds like 2,3-butanedione. This compound has been also confirmed as being dependent on the wine bacteria used.
Computing Effective Permeability of Porous Media with FEM and Micro-CT: An Educational Approach
Authors: Vianna Rafael S. , Cunha Alexsander M. , Azeredo Rodrigo B. V. , Leiderman Ricardo , Pereira Andre
Published: January 2020

Permeability is a parameter that measures the resistance that fluid faces when flowing through a porous medium. Usually, this parameter is determined in routine laboratory tests by applying Darcy’s law. Those tests can be complex and time-demanding, and they do not offer a deep understanding of the material internal microstructure. Currently, with the development of new computational technologies, it is possible to simulate fluid flow experiments in computational labs. Determining permeability with this strategy implies solving a homogenization problem, where the determination of the macro parameter relies on the simulation of a fluid flowing through channels created by connected pores present in the material’s internal microstructure. This is a powerful example of the application of fluid mechanics to solve important industrial problems (e.g., material characterization), in which the students can learn basic concepts of fluid flow while practicing the implementation of computer simulations. In addition, it gives the students a concrete opportunity to work with a problem that associates two different scales. In this work, we present an educational code to compute absolute permeability of heterogeneous materials. The program simulates a Stokes flow in the porous media modeled with periodic boundary conditions using finite elements. Lastly, the permeability of a real sample of sandstone, modeled by microcomputed tomography (micro-CT), is obtained.
Development of a Real-Time PCR Assay for the Detection of Donkey (Equus asinus) Meat in Meat Mixtures Treated under Different Processing Conditions
Authors: Kim Mi-Ju , Suh Seung-Man , Kim Sung-Yeon , Qin Pei , Kim Hong-Rae , Kim Hae-Yeong
Published: January 2020

In this study, a donkey-specific primer pair and probe were designed from mitochondrial cytochrome b gene for the detection of raw donkey meat and different processed meat mixtures. The PCR product size for donkey DNA was 99 bp, and primer specificity was verified using 20 animal species. The limit of detection (LOD) was examined by serially diluting donkey DNA. Using real-time PCR, 0.001 ng of donkey DNA could be detected. In addition, binary meat mixtures with various percentages of donkey meat (0.001%, 0.01%, 0.1%, 1%, 10%, and 100%) in beef were analyzed to determine the sensitivity of this real-time PCR assay. At least 0.001% of donkey meat was detected in raw, boiled, roasted, dried, grinded, fried, and autoclaved meat mixtures. The developed real-time PCR method showed sufficient specificity and sensitivity in identification of donkey meat and could be a useful tool for the identification of donkey meat in processed products.
Non-Starch Polysaccharides in Wheat Beers and Barley Malt beers: A Comparative Study
Authors: Li Miaomiao , Du Jinhua , Zheng Yaxin
Published: January 2020

Non-starch polysaccharides (NSPs) in beers attract extensive attention due to their health benefits. The aim of this work was to investigate and compare NSPs including arabinoxylan, arabinogalactan, β–glucans, and mannose polymers in wheat and barley malt beers as well as the influence on its quality. NSPs in wheat beers (1953–2923 mg/L) were higher than that in barley malt beers (1442–1756 mg/L). Arabinoxylan was the most abundant followed by arabinogalactan. In contrast to barley malt beers, wheat beers contained more mannose polymers (130–182 mg/L) than β-glucan (26–99 mg/L), indicating that more arabinoxylan, arabinogalactan, and mannose polymers came from wheat malt. The substitution degree of arabinoxylan in wheat beers (0.57–0.66) was lower than that in barley malt beers (0.68–0.72), while the degree of polymerization (38–83) was higher (p < 0.05) than that in barley malt beers (38–48), indicating different structures of arabinoxylan derived from barley malt and wheat malt. NSPs, especially arabinoxylan content, positively correlated (p < 0.01) with real extract and viscosity of beers. Furthermore, wheat and barley malt beers were well separated in groups by principal component analysis.
Food Supply Chain and Business Model Innovation
Authors: Nosratabadi Saeed , Mosavi Amirhosein , Lakner Zoltan
Published: January 2020

This paper investigates the contribution of business model innovations in the advancement of novel food supply chains. Through a systematic literature review, the notable business model innovations in the food industry are identified, surveyed, and evaluated. Findings reveal that the innovations in value proposition, value creation processes, and value delivery processes of business models are the successful strategies proposed in food industry. It is further disclosed that rural female entrepreneurs, social movements, and also urban conditions are the most important driving forces causing farmers to reconsider their business models. In addition, the new technologies and environmental factors are the secondary contributors in business model innovation for the food processors. It is concluded that digitalization has disruptively changed the food distributor models. E-commerce models and Internet-of-Things are reported as the essential factors causing retailers to innovate their business models. Furthermore, consumption demand and product quality are two main factors affecting the business models of all the firms operating in the food supply chain regardless of their positions in the chain. The findings of the current study provide an insight into the food industry to design a sustainable business model to bridge the gap between food supply and food demand.
Prader–Willi-Like Phenotype Caused by an Atypical 15q11.2 Microdeletion
Authors: Tan Qiming , Potter Kathryn J. , Burnett Lisa Cole , Orsso Camila E. , Inman Mark , Ryman Davis C. , Haqq Andrea M.
Published: January 2020

We report a 17-year-old boy who met most of the major Prader–Willi syndrome (PWS) diagnostic criteria, including infantile hypotonia and poor feeding followed by hyperphagia, early-onset morbid obesity, delayed development, and characteristic facial features. However, unlike many children with PWS, he had spontaneous onset of puberty and reached a tall adult stature without growth hormone replacement therapy. A phenotype-driven genetic analysis using exome sequencing identified a heterozygous microdeletion of 71 kb in size at chr15:25,296,613-25,367,633, genome build hg 19. This deletion does not affect the SNURF-SNRPN locus, but results in the loss of several of the PWS-associated non-coding RNA species, including the SNORD116 cluster. We compared with six previous reports of patients with PWS who carried small atypical deletions encompassing the snoRNA SNORD116 cluster. These patients share similar core symptoms of PWS while displaying some atypical features, suggesting that other genes in the region may make lesser phenotypic contributions. Altogether, these rare cases provide convincing evidence that loss of the paternal copy of the SNORD116 snoRNA is sufficient to cause most of the major clinical features of PWS.
Quality, Yield, and Biomass Efficacy of Several Hydroponic Lettuce (Lactuca sativa L.) Cultivars in Response to High Pressure Sodium Lights or Light Emitting Diodes for Greenhouse Supplemental Lighting
Authors: Hernandez Erica , Timmons Michael B. , Mattson Neil S.
Published: January 2020

Lettuce is an economically important crop that can be grown either in the field or greenhouse. Different challenges are present in either environment; therefore, cultivar selection is important. For hydroponic greenhouse lettuce there is relatively little published information on cultivar selection under different lighting sources. The objective of phase 1 was to determine the influence of lighting using high pressure sodium (HPS) or light emitting diodes (LED) on plant fresh weight, height, tip burn index, bolting, and Brix. Phase 2 was similar to phase 1 but with fewer cultivars (1) to allow for greater number of replicates per treatment per crop cycle. Each experiment consisted of three crop cycles over time per phase. Light sources were controlled using an algorithm, Light and Shade System Implementation (LASSI), to achieve a constant average daily light integral under each treatment and crop cycle. Electrical consumption and efficacy (fresh weight per kWh) from each treatment was estimated using data collected on power consumption from representative lamps multiplied by the number of fixtures and the hours fixtures were on per crop cycle. In phase 1, the fresh weight of 2 to 3 cultivars was greater under HPS and 1 to 2 cultivars under LED, depending on production cycle. The HPS-grown lettuce tended to have more tip burn and bolting in crop cycles 1 and 2, with cycle 3 showing similar tip burn incidence. Bolting was only consistently observed in one cultivar, ‘Teodore’. The LED array used less than half as much electricity as the HPS array, while producing relatively similar size lettuce, therefore, leading to electrical efficacies two- to three-times higher in LED than in HPS treatments. In phase 2, significant differences in height were found in ‘Greenstar’ and ‘Xandra’, with HPS producing larger plants than LED. Significant differences were also found in diameter in ‘Greenstar’, ‘Xandra’, ‘Locarno’, and ‘Crunchita’, with HPS again being larger than LED.
Physical Activity-Related Injuries and Risk Factors among Secondary School Students in Hong Kong
Authors: Cai Weicong , Sun Yan , Peng Ke , Kwok Heather , Lei Lin , Wu Shing , Lam Chi Kei , Li Liping , Gao Yang
Published: January 2020

Increase in physical activity (PA) might elevate risks for physical activity-related injuries (PARIs). This study aimed to investigate the incidence rate and risk factors for PARIs among secondary school students in Hong Kong. All eligible students from three secondary schools were invited to participate in the study from November to December 2014. Information on PARI occurrences in the past 12 months, PA participation, and sociodemographics was collected. Multivariate and hierarchical logistic regression models were performed to estimate the risk of potential factors for PARIs. In total, 1916 students in forms 1–6 (aged 14.3 ± 1.7 years) completed valid questionnaires, with an overall yearly PARI incidence rate of 32.1% (boys: 34.3%; girls: 29.3%). There were sex differences in the risk factors identified, except that family size was associated with PARIs for all participants. Longer duration and higher intensity of PA increased the risk for PARI occurrences in both sexes. In conclusion, PARIs were prevalent among secondary school students in Hong Kong, and different sexes had different PARI incidence rates and were influenced by different risk factors. There is an urgent call for effective sex-specific interventions to prevent PARIs in this population.
Prevention of Disasters Related to Extreme Natural Ground Deformation Events by Applying Spatial Modeling in Urban Areas (Quito, Ecuador)
Authors: Cando-Jácome Marcelo , Martínez-Graña Antonio , Valdés Virginia
Published: January 2020

Synthetic Aperture Radar Interferometry (InSAR) is a spatial technique based on obtaining the phase differences of two radar images, acquired by a satellite from separate orbits and at different times, to obtain a ground displacement image of a study area, This image is called interferogram. On the other hand, space syntax is a technique within architecture that is applied to quantify and describe the level of ease of population movement through any urban space in a city. It analyzes the flow, transit, displacement, accessibility and concentration of the population in areas of basic services, health, security, commerce and entertainment. What would happen if an earthquake greater than 6 or 7 Moment Magnitude-Mw occurs in these areas of intense concentration of the population that are in buildings constructed on intense deformations of the land? With respect to the seismic risk in the city of Quito, many studies related to seismic risks have been published, but there are no studies that relate the deformation of the land (INSAR) with the space syntax, so this article presents a new vision in the joint application of these tools, a useful vision for urban planners and designers, considering the occurrence of a major earthquake in areas of buildings that are located on intense land deformations and have high population concentrations. This study has been prepared in two phases: in the first phase, the built-up areas concentrated in the greatest terrain deformations by accumulated displacement obtained using the APS estimation & multitemporal analysis by PSI-InSAR time series analysis methodology and Sentinel 1A and 1B satellite images were categorized. In the second phase, through the space syntax’s theory and the use of DepthmapX, the movement patterns and traffic flows of the population were determined by means of graphs of spaces interconnected by streets (axial maps), to predict the spatial behavior of humans and its concentration in the mentioned sites. Finally, the results were integrated, determining the degree of exposure of the population found in built areas with high to very high displacement and an intense population concentration.
The Predictive Role of Raw Bioelectrical Impedance Parameters in Water Compartments and Fluid Distribution Assessed by Dilution Techniques in Athletes
Authors: Francisco Ruben , Matias Catarina N. , Santos Diana A. , Campa Francesco , Minderico Claudia S. , Rocha Paulo , Heymsfield Steven B. , Lukaski Henry , Sardinha Luís B. , Silva Analiza M.
Published: January 2020

The aims of this study were to analyze the usefulness of raw bioelectrical impedance (BI) parameters in assessing water compartments and fluid distribution in athletes. A total of 202 men and 71 female athletes were analyzed. Total body water (TBW) and extracellular water (ECW) were determined by dilution techniques, while intracellular water (ICW) was calculated. Fluid distribution was calculated as the ECW/ICW ratio (E:I). Phase angle (PhA), resistance (R) and reactance (Xc) were obtained through BI spectroscopy using frequency 50kHz. Fat (FM) and fat-free mass (FFM) were assessed by dual-energy X-ray absorptiometry. After adjusting for height, FM, FFM, age and sports category we observed that: PhA predicted ICW (females: β = 1.62, p < 0.01; males: β = 2.70, p < 0.01) and E:I (males and females: β = −0.08; p < 0.01); R explained TBW (females: β = −0.03; p < 0.01; males: β = −0.06; p < 0.01) and ECW (females: β = –0.02, p < 0.01; males: β = −0.03, p < 0.01) and ICW (females: β = –0.01, p < 0.053; males: β = –0.03 p < 0.01); and Xc predicted ECW (females: β = −0.06, p < 0.01; males: β = −0.12, p < 0.01). A higher PhA is a good predictor of a larger ICW pool and a lower E:I, regardless of body composition, age, height, and sports category. Lower R is associated with higher water pools whereas ECW expansion is explained by lower Xc. Raw BI parameters are useful predictors of total and extracellular pools, cellular hydration and fluid distribution in athletes.
Buddhism and Depressive Symptoms among Married Women in Urban Thailand
Authors: Xu Ting , Xu Xiaohe , Sunil Thankam , Sirisunyaluck Bangon
Published: January 2020

A growing body of research has documented salutary associations between religious involvement and poor mental health outcomes, such as depressive symptoms and psychological distress. However, little scholarly attention has been given to the association between Buddhism, a non-Western religious faith, and depressive symptomatology in Thailand. Using random survey data collected from urban Thailand, this study examines the association between religious involvement and depressive symptoms among married women in Bangkok. Findings from multiple linear regression models reveal that (1) Buddhist respondents report significantly lower levels of depressive symptoms than their non-Buddhist counterparts, (2) the frequency of participation in religious activities is significantly and inversely associated with the level of depressive symptoms, and (3) the inverse association between religious participation and depressive symptoms is more salient for Buddhists who frequently practice their faith (i.e., significant interaction effect). Research limitations and directions for future research are discussed.
Prevalence and Factors Associated with Driving Under the Influence of Alcohol in Brazil: An Analysis by Macroregion
Authors: Guimarães Rafael Alves , Morais Neto Otaliba Libânio
Published: January 2020

Objective: To analyze the prevalence and factors associated with driving under the influence of alcohol (DUIA) in Brazil, according to macroregion. Methods: A cross-sectional study was conducted using data from individuals aged 18 years or older who participated in the 2013 National Health Survey in Brazil. Subjects were selected by probabilistic sampling and interviewed through home visits. Prevalence of DUIA was estimated according to the number of drivers and/or motorcyclists who reported consuming alcohol in the previous 30 days (n = 9537). Poisson regression was used to analyze the factors associated with DUIA to Brazil and in each macroregion of the country. Results: The prevalence of DUIA was 27.5%, 29.4%, 29.6%, 22.9%, and 20.8% in the North, Northeast, Central-West, South, and Southeast macroregions, respectively. The overall prevalence of Brazil was 24.3%. In most macroregions, the main predictors of DUIA were male sex, high educational level, living in outside the capital or metropolitan regions (other regions), and binge drinking in the previous 30 days. Depression was a predictor in Brazil and two macroregions. Conclusion: A high prevalence of DUIA was observed in Brazil, especially in the Central-West, Northeast and, North macro-regions. Factors associated with DUIA can be incorporated to develop effective interventions to reduce this behavior in Brazil.
Built Environments and Cardiometabolic Morbidity and Mortality in Remote Indigenous Communities in the Northern Territory, Australia
Authors: Le Gal Camille , Dale Michael J. , Cargo Margaret , Daniel Mark
Published: January 2020

The health of Indigenous Australians is dramatically poorer than that of the non-Indigenous population. Amelioration of these differences has proven difficult. In part, this is attributable to a conceptualisation which approaches health disparities from the perspective of individual-level health behaviours, less so the environmental conditions that shape collective health behaviours. This ecological study investigated associations between the built environment and cardiometabolic mortality and morbidity in 123 remote Indigenous communities representing 104 Indigenous locations (ILOC) as defined by the Australian Bureau of Statistics. The presence of infrastructure and/or community buildings was used to create a cumulative exposure score (CES). Records of cardiometabolic-related deaths and health service interactions for the period 2010–2015 were sourced from government department records. A quasi-Poisson regression model was used to assess the associations between built environment “healthfulness” (CES, dichotomised) and cardiometabolic-related outcomes. Low relative to high CES was associated with greater rates of cardiometabolic-related morbidity for two of three morbidity measures (relative risk (RR) 2.41–2.54). Cardiometabolic-related mortality was markedly greater (RR 4.56, 95% confidence interval (CI), 1.74–11.93) for low-CES ILOCs. A lesser extent of “healthful” building types and infrastructure is associated with greater cardiometabolic-related morbidity and mortality in remote Indigenous locations. Attention to environments stands to improve remote Indigenous health.
Perspectives for Future Use of Extracellular Vesicles from Umbilical Cord- and Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells in Regenerative Therapies—Synthetic Review
Authors: Lelek Joanna , Zuba-Surma Ewa K.
Published: January 2020

Mesenchymal stem/ stromal cells (MSCs) represent progenitor cells of various origin with multiple differentiation potential, representing the most studied population of stem cells in both in vivo pre-clinical and clinical studies. MSCs may be found in many tissue sources including extensively studied adipose tissue (ADSCs) and umbilical cord Wharton’s jelly (UC-MSCs). Most of sanative effects of MSCs are due to their paracrine activity, which includes also release of extracellular vesicles (EVs). EVs are small, round cellular derivatives carrying lipids, proteins, and nucleic acids including various classes of RNAs. Due to several advantages of EVs when compare to their parental cells, MSC-derived EVs are currently drawing attention of several laboratories as potential new tools in tissue repair. This review focuses on pro-regenerative properties of EVs derived from ADSCs and UC-MSCs. We provide a synthetic summary of research conducted in vitro and in vivo by employing animal models and within initial clinical trials focusing on neurological, cardiovascular, liver, kidney, and skin diseases. The summarized studies provide encouraging evidence about MSC-EVs pro-regenerative capacity in various models of diseases, mediated by several mechanisms. Although, direct molecular mechanisms of MSC-EV action are still under investigation, the current growing data strongly indicates their potential future usefulness for tissue repair.
Deficiency in Dipeptidyl Peptidase-4 Promotes Chemoresistance Through the CXCL12/CXCR4/mTOR/TGFβ Signaling Pathway in Breast Cancer Cells
Authors: Li Shaolan , Fan Yang , Kumagai Asako , Kawakita Emi , Kitada Munehiro , Kanasaki Keizo , Koya Daisuke
Published: January 2020

Dipeptidyl peptidase (DPP)-4, a molecular target of DPP-4 inhibitors, which are type 2 diabetes drugs, is expressed in a variety of cell types, tissues and organs. DPP-4 has been shown to be involved in cancer biology, and we have recently shown that a DPP-4 inhibitor promoted the epithelial mesenchymal transition (EMT) in breast cancer cells. The EMT is known to associate with chemotherapy resistance via the induction of ATP-binding cassette (ABC) transporters in cancer cells. Here, we demonstrated that deficiency in DPP-4 promoted chemotherapy resistance via the CXCL12/CXCR4/mTOR axis, activating the TGFβ signaling pathway via the expression of ABC transporters. DPP-4 inhibition enhanced ABC transporters in vivo and in vitro. Doxorubicin (DOX) further induced ABC transporters in DPP-4-deficient 4T1 cells, and the induction of ABC transporters was suppressed by either the CXCR4 inhibitor AMD3100, the mTOR inhibitor rapamycin or a neutralizing TGFβ (1, 2 and 3) antibody(N-TGFβ). Knockdown of snail, an EMT-inducible transcription factor, suppressed ABC transporter levels in DOX-treated DPP-4-deficient 4T1 cells. In an allograft mouse model, however, the effects of DOX in either primary tumor or metastasis were not statistically different between control and DPP-4-kd 4T1. Taken together, our findings suggest that DPP-4 inhibitors potentiate chemotherapy resistance via the induction of ABC transporters by the CXCL12/CXCR4/mTOR/TGFβ signaling pathway in breast cancer cells.
The Effects of Early-Onset Pre-Eclampsia on Placental Creatine Metabolism in the Third Trimester
Authors: Ellery Stacey J. , Murthi Padma , Della Gatta Paul A. , May Anthony K. , Davies-Tuck Miranda L. , Kowalski Greg M. , Callahan Damien L. , Bruce Clinton R. , Wallace Euan M. , Walker David W. , Dickinson Hayley , Snow Rod J.
Published: January 2020

Creatine is a metabolite important for cellular energy homeostasis as it provides spatio-temporal adenosine triphosphate (ATP) buffering for cells with fluctuating energy demands. Here, we examined whether placental creatine metabolism was altered in cases of early-onset pre-eclampsia (PE), a condition known to cause placental metabolic dysfunction. We studied third trimester human placentae collected between 27–40 weeks’ gestation from women with early-onset PE (n = 20) and gestation-matched normotensive control pregnancies (n = 20). Placental total creatine and creatine precursor guanidinoacetate (GAA) content were measured. mRNA expression of the creatine synthesizing enzymes arginine:glycine aminotransferase (GATM) and guanidinoacetate methyltransferase (GAMT), the creatine transporter (SLC6A8), and the creatine kinases (mitochondrial CKMT1A & cytosolic BBCK) was assessed. Placental protein levels of arginine:glycine aminotransferase (AGAT), GAMT, CKMT1A and BBCK were also determined. Key findings; total creatine content of PE placentae was 38% higher than controls (p < 0.01). mRNA expression of GATM (p < 0.001), GAMT (p < 0.001), SLC6A8 (p = 0.021) and BBCK (p < 0.001) was also elevated in PE placentae. No differences in GAA content, nor protein levels of AGAT, GAMT, BBCK or CKMT1A were observed between cohorts. Advancing gestation and birth weight were associated with a down-regulation in placental GATM mRNA expression, and a reduction in GAA content, in control placentae. These relationships were absent in PE cases. Our results suggest PE placentae may have an ongoing reliance on the creatine kinase circuit for maintenance of cellular energetics with increased total creatine content and transcriptional changes to creatine synthesizing enzymes and the creatine transporter. Understanding the functional consequences of these changes warrants further investigation.
Rosemary Extract Inhibits Proliferation, Survival, Akt, and mTOR Signaling in Triple-Negative Breast Cancer Cells
Authors: Jaglanian Alina , Tsiani Evangelia
Published: January 2020

Breast cancer is the most commonly diagnosed cancer in women. Triple-negative (TN) breast cancer lacks expression of estrogen receptor (ER), progesterone receptor (PR) as well as the expression and/or gene amplification of human epidermal growth factor receptor 2 (HER2). TN breast cancer is aggressive and does not respond to hormone therapy, therefore new treatments are urgently needed. Plant-derived chemicals have contributed to the establishment of chemotherapy agents. In previous studies, rosemary extract (RE) has been found to reduce cell proliferation and increase apoptosis in some cancer cell lines. However, there are very few studies examining the effects of RE in TN breast cancer. In the present study, we examined the effects of RE on TN MDA-MB-231 breast cancer cell proliferation, survival/apoptosis, Akt, and mTOR signaling. RE inhibited MDA-MB-231 cell proliferation and survival in a dose-dependent manner. Furthermore, RE inhibited the phosphorylation/activation of Akt and mTOR and enhanced the cleavage of PARP, a marker of apoptosis. Our findings indicate that RE has potent anticancer properties against TN breast cancer and modulates key signaling molecules involved in cell proliferation and survival.
Microglial Activation in the Retina of a Triple-Transgenic Alzheimer’s Disease Mouse Model (3xTg-AD)
Authors: Salobrar-García Elena , Rodrigues-Neves Ana C. , Ramírez Ana I. , de Hoz Rosa , Fernández-Albarral José A. , López-Cuenca Inés , Ramírez José M. , Ambrósio António F. , Salazar Juan J.
Published: January 2020

Alzheimer’s disease (AD) is the most common type of dementia in the world. The main biomarkers associated with AD are protein amyloid-β (Aβ) plaques and protein tau neurofibrillary tangles, which are responsible for brain neuroinflammation mediated by microglial cells. Increasing evidence has shown that the retina can also be affected in AD, presenting some molecular and cellular changes in the brain, such as microglia activation. However, there are only a few studies assessing such changes in the retinal microglia in animal models of AD. These studies use retinal sections, which have some limitations. In this study, we performed, for the first time in a triple-transgenic AD mouse model (3xTg-AD), a quantitative morphometric analysis of microglia activation (using the anti-Iba-1 antibody) in retinal whole-mounts, allowing visualization of the entire microglial cell, as well as its localization along the extension of the retina in different layers. Compared to age-matched animals, the retina of 3xTg-AD mice presents a higher number of microglial cells and a thicker microglial cell body area. Moreover, the microglia migrate, reorient, and retract their processes, changing their localization from a parallel to a perpendicular position relative to the retinal surface. These findings demonstrate clear microglia remodeling in the retina of 3xTg-AD mice.
Evaluating Ligand Modifications of the Titanocene and Auranofin Moieties for the Development of More Potent Anticancer Drugs
Authors: Fernández-Vega Lauren , Ruiz Silva Valeria A. , Domínguez-González Tania M. , Claudio-Betancourt Sebastián , Toro-Maldonado Rafael E. , Capre Maso Luisa C. , Sanabria Ortiz Karina , Pérez-Verdejo Jean A. , Román González Janeishly , Rosado-Fraticelli Grecia T. , Pagán Meléndez Fabiola , Betancourt Santiago Fabiola M. , Rivera-Rivera Daniel A. , Martínez Navarro Carlos , Bruno Chardón Andrea C. , Vera Axel O. , Tinoco Arthur D.
Published: January 2020

Over time platinum-based anticancer drugs have dominated the market, but their side effects significantly impact the quality of life of patients. Alternative treatments are being developed all over the world. The titanocene and auranofin families of compounds, discovered through an empirical search for other metal-based therapeutics, hold tremendous promise to improve the outcomes of cancer treatment. Herein we present a historical perspective of these compounds and review current efforts focused on the evolution of their ligands to improve their physiological solution stability, cancer selectivity, and antiproliferative performance, guided by a clear understanding of the coordination chemistry and aqueous speciation of the metal ions, of the cytotoxic mechanism of action of the compounds, and the external factors that limit their therapeutic potential. Newer members of these families of compounds and their combination in novel bimetallic complexes are the result of years of scientific research. We believe that this review can have a positive impact in the development and understanding of the metal-based drugs of gold, titanium, and beyond.