bluuebunny/crossref_metadata_2025 huggingface dataset:
iPSC-Derived Astrocytes and Neurons Replicate Brain Gene Expression, Epigenetic, Cell Morphology and Connectivity Alterations Found in Autism
Authors: Mostafavi Abdolmaleky Hamid , Alam Reza , Nohesara Shabnam , Deth Richard C. , Zhou Jin-Rong
Published: June 2024

Excessive inflammatory reactions and oxidative stress are well-recognized molecular findings in autism and these processes can affect or be affected by the epigenetic landscape. Nonetheless, adequate therapeutics are unavailable, as patient-specific brain molecular markers for individualized therapies remain challenging. Methods: We used iPSC-derived neurons and astrocytes of patients with autism vs. controls (5/group) to examine whether they replicate the postmortem brain expression/epigenetic alterations of autism. Additionally, DNA methylation of 10 postmortem brain samples (5/group) was analyzed for genes affected in PSC-derived cells. Results: We found hyperexpression of TGFB1, TGFB2, IL6 and IFI16 and decreased expression of HAP1, SIRT1, NURR1, RELN, GPX1, EN2, SLC1A2 and SLC1A3 in the astrocytes of patients with autism, along with DNA hypomethylation of TGFB2, IL6, TNFA and EN2 gene promoters and a decrease in HAP1 promoter 5-hydroxymethylation in the astrocytes of patients with autism. In neurons, HAP1 and IL6 expression trended alike. While HAP1 promoter was hypermethylated in neurons, IFI16 and SLC1A3 promoters were hypomethylated and TGFB2 exhibited increased promoter 5-hydroxymethlation. We also found a reduction in neuronal arborization, spine size, growth rate, and migration, but increased astrocyte size and a reduced growth rate in autism. In postmortem brain samples, we found DNA hypomethylation of TGFB2 and IFI16 promoter regions, but DNA hypermethylation of HAP1 and SLC1A2 promoters in autism. Conclusion: Autism-associated expression/epigenetic alterations in iPSC-derived cells replicated those reported in the literature, making them appropriate surrogates to study disease pathogenesis or patient-specific therapeutics.
Daily Light Onset and Plasma Membrane Tethers Regulate Mitochondria Redistribution within the Retinal Pigment Epithelium
Authors: Neto Matilde V. , De Rossi Giulia , Berkowitz Bruce A. , Seabra Miguel C. , Luthert Philip J. , Futter Clare E. , Burgoyne Thomas
Published: June 2024

The retinal pigment epithelium (RPE) is an essential component of the retina that plays multiple roles required to support visual function. These include light onset- and circadian rhythm-dependent tasks, such as daily phagocytosis of photoreceptor outer segments. Mitochondria provide energy to the highly specialized and energy-dependent RPE. In this study, we examined the positioning of mitochondria and how this is influenced by the onset of light. We identified a population of mitochondria that are tethered to the basal plasma membrane pre- and post-light onset. Following light onset, mitochondria redistributed apically and interacted with melanosomes and phagosomes. In a choroideremia mouse model that has regions of the RPE with disrupted or lost infolding of the plasma membrane, the positionings of only the non-tethered mitochondria were affected. This provides evidence that the tethering of mitochondria to the plasma membrane plays an important role that is maintained under these disease conditions. Our work shows that there are subpopulations of RPE mitochondria based on their positioning after light onset. It is likely they play distinct roles in the RPE that are needed to fulfil the changing cellular demands throughout the day.
Acute Respiratory Tract Infections (ARTIs) in Children after COVID-19-Related Social Distancing: An Epidemiological Study in a Single Center of Southern Italy
Authors: Falsaperla Raffaele , Sortino Vincenzo , La Cognata Daria , Barberi Chiara , Corsello Giovanni , Malaventura Cristina , Suppiej Agnese , Collotta Ausilia Desiree , Polizzi Agata , Grassi Patrizia , Ruggieri Martino
Published: June 2024

In Sicily (Italy), respiratory syncytial virus (RSV), rhinovirus (HRV), and influenza virus triggered epidemics among children, resulting in an increase in acute respiratory tract infections (ARTIs). Our objective was to capture the epidemiology of respiratory infections in children, determining which pathogens were associated with respiratory infections following the lockdown and whether there were changes in the epidemiological landscape during the post-SARS-CoV-2 pandemic era. Materials and Methods: We analyzed multiplex respiratory viral PCR data (BioFire® FilmArray® Respiratory Panel 2.1 Plus) from 204 children presenting with respiratory symptoms and/or fever to our Unit of Pediatrics and Pediatric Emergency. Results: Viruses were predominantly responsible for ARTIs (99%), with RSV emerging as the most common agent involved in respiratory infections, followed by human rhinovirus/enterovirus and influenza A. RSV and rhinovirus were also the primary agents in coinfections. RSV predominated during winter months, while HRV/EV exhibited greater prevalence than RSV during the fall. Some viruses spread exclusively in coinfections (human coronavirus NL63, adenovirus, metapneumovirus, and parainfluenza viruses 1–3), while others primarily caused mono-infections (influenza A and B). SARS-CoV-2 was detected equally in both mono-infections (41%) and coinfections (59%). Conclusions: Our analysis underlines the predominance of RSV and the importance of implementing preventive strategies for RSV.
The Effect of Directional Tactile Memory of the Back of the User on Reaction Time and Accuracy
Authors: Elshafei Ali , Romano Daniela , Fahim Irene S.
Published: June 2024

Tactile memory is the cognitive process of storing and recalling information that has been perceived through the sense of touch. Directional tactile memory involves the encoding and retrieval of sensory data associated with a tactile experience, allowing individuals to remember and recognize directional information encoded through the sense of touch. A new method for providing directional tactile feedback, at the back of the user, has been developed to investigate the efficacy of directional tactile memory, its decay over time, and its impact during a concurrent cognitive task. Two experiments were presented. In the first experiment, tactile memory deterioration, with a visual or a tactile cue, was tested with different action-cue latencies (10 s and 20 s). In the second experiment, we considered tactile memory deterioration when there was an increased cognitive load as the participants played Tetris. Forty volunteers participated in the two experiments using purpose-built tactile seats with nine motors controlled by an Arduino. The performance data (error and reaction times) were analyzed statistically, and a NASA task load index (NASA-TLX) questionnaire was administered to measure the subjective workload after each of the two experiments. The findings highlighted that the directional tactile memory of the back can guide individuals to the correct point on the screen and that it can be maintained for at least 20 s. There was no statistically significant difference in the number of errors or reaction time with a visual or tactile action cue. However, being involved in a concurrent cognitive task (playing Tetris) adversely affected the reaction time, the number of errors, and the directional tactile memory, which degraded as the time between the directional cue and the action cue increased. Participants perceived the performance while playing Tetris as significantly more mentally and perceptually demanding, requiring more mental and physical effort and being more frustrating. These trials revealed a new potential for a human–machine interface system, leveraging directional tactile memory, which might be utilized to increase the safety of autonomous vehicles.
Advancing Small and Medium-Sized Enterprise Manufacturing: Framework for IoT-Based Data Collection in Industry 4.0 Concept
Authors: Barton Martin , Budjac Roman , Tanuska Pavol , Sladek Ivan , Nemeth Martin
Published: June 2024

Currently, industrial engineers are witnessing a rapid development of innovation in modern industry and the integration of critical elements of Industry 4.0 into production processes in order to remain competitive. Such changes are characterized by a large amount of effort and financial resources. To successfully deploy these changes requires not only the purchase of hardware and software but also the training of employees and the modification of the company’s organizational structure. The main objective of the article is to propose a framework for the modernization of SMEs to a level close to the Smart Factory by using the necessary attributes of Industry 4.0. The framework design is based on the initial state of a standard SME and consists of the design of fitting new IoT devices for efficient data collection, the design of a data warehouse for storing process data using Hadoop, and the integration of process- and operational-level data into the prepared data warehouse. The resulting design is developed in the form of a methodology and is generalized for use in manufacturing enterprises. The universal design is independent of the initial state of the enterprise.
Performance Evaluation and Working Fluid Screening of Direct Vapor Generation for Solar ORC Using Low-Global Warming Potential (GWP) Working Fluids
Authors: Jiang Youtao , Zhang Xunda , Zhang Zhengao , Hao Lei , Cao Zhaozhi , Li Shuyang , Guo Bowen , Zheng Yawen , Dong Chunhai , Zhao Li
Published: June 2024

Traditional working fluids used in direct vapor generation for solar organic Rankine cycle (DVG-ORC) systems have a high global warming potential (GWP), making it imperative to find environmentally friendly alternative working fluids for these systems. This paper evaluates the performance of the DVG-ORC system under different operating conditions. By comparing the results of traditional working fluids with those of low-GWP fluids, the feasibility of using low-GWP fluids as alternative working fluids is explored. Additionally, to screen the working fluids suitable for this system further, the system is optimized with net output power as the objective function. The results show that evaporation temperature has different impacts on system performance. R245ca and R1336mzz(Z) exhibit higher net output power at different evaporation temperatures, with R1336mzz(Z) only reducing it by 3.73–5.26% compared to R245ca. However, an increase in condensation temperature negatively affects system performance, leading to a decrease in net output power and various efficiencies. Net output power increases with an increase in mass flow rate, indicating that higher mass flow rates can enhance system performance. The optimization results show that the net output power of low-GWP working fluid R1336mzz(Z) decreases by only 3.44% compared to R245ca, which achieves the maximum net output power. Moreover, among low-GWP working fluids, R1336mzz(Z) demonstrates the highest ORC efficiency and system efficiency, making it the most suitable working fluid for the DVG-ORC system due to its environmental friendliness and safety.
Effects of Acute Temperature Stress on the Expression of Related Genes in the Brain of Opsariichthys bidens
Authors: Li Qianhui , Xiong Luomei , Zhu Yechen , Zheng Anrui , Zheng Shanjian
Published: June 2024

Opsariichthys bidens (O. bidens) is a fish species native to China and sensitive to temperature changes. In this study, the effects of acute temperature stress on brain gene expression in O. bidens were investigated by sampling brain tissues from specimens exposed to three different temperatures (15 °C, 25 °C, and 35 °C) for varying durations of 2 h, 4 h, 6 h, and 8 h. The study focused on analyzing the expression patterns of key genes implicated in neural function and stress response, including brain-derived neurotrophic factor (BDNF), c-FOS, heat shock proteins (HSP70, HSP90), endoplasmic reticulum stress markers (IRE1, GRP78), oxidative stress enzymes (CAT, SOD), and apoptotic regulators (caspase3, Bax). The findings revealed that upon exposure to acute heat stress, the expression levels of the aforementioned genes in the brain of O. bidens were up-regulated within 2 h, peaking at the 4-h mark. Conversely, following acute cold stress, the expression of c-FOS, BDNF, HSP70, HSP90, SOD, and CAT genes increased significantly after 4 h, while caspase3 expression was notably elevated at the 6-h mark, with no significant impact observed on Bax, IRE1, or GRP78 gene expression levels. The study suggested that the brain of O. bidens responds to high temperatures through mechanisms involving neural activation, heat shock proteins, endoplasmic reticulum stress, oxidative stress, and apoptosis. Similarly, adaptation to low temperatures by O. bidens’ brain was associated with neural activation, regulation of heat shock proteins, oxidative stress responses, and apoptotic processes. Overall, this research aimed to elucidate the impact of temperature stress on brain physiology and the adaptive mechanisms of O. bidens at the genetic level, providing a foundational understanding of its temperature adaptation strategies.
Advances in the Preparation, Structure and Bioactivity of Polysaccharides from Lycium ruthenicum Murr.: A Review
Authors: Liu Bing , Ma Jingyu , Li Ting , Li Pei , Yan Dehui , Zhu Jun , Zhang Xinguo
Published: June 2024

Lycium ruthenicum Murr. is rich in polysaccharides, and the polysaccharides in Lycium ruthenicum Murr. (LRPS) have various bioactivities, such as antioxidant activity, anti-tumor activity, neuroprotective activity, and immunomodulatory activity. It has broad prospects in the development of functional foods and pharmaceuticals. Researchers have found that the structural characteristics of LRPS, such as molecular weight, monosaccharide composition, primary structure, etc., have a significant impact on their bioactivities. Therefore, studying the structure of LRPS is of great significance in revealing their bioactivities and mechanisms. This study, based on introducing the preparation methods of LRPS, focuses on reviewing the research progress on the main structural characteristics, various bioactivities, and mechanisms of action of LRPS. In addition, the study provides prospects for the development of LRPS in the fields of food and medicine, aiming to provide theoretical support for its deep processing and application.
The Effect of Recycled Spent Coffee Grounds Fertilizer, Vermicompost, and Chemical Fertilizers on the Growth and Soil Quality of Red Radish (Raphanus sativus) in the United Arab Emirates: A Sustainability Perspective
Authors: Mesmar Athari K. , Albedwawi Shaikha T. , Alsalami Aysha K. , Alshemeili Alreem R. , Abu-Elsaoud Abdelghafar M. , El-Tarabily Khaled A. , Al Raish Seham M.
Published: June 2024

The overuse of chemical fertilizers degrades the soil ecosystem and restricts the natural development of plants. Various byproducts are produced throughout the production and consumption of coffee within the coffee industry, and they are significant in terms of environmental waste. Spent coffee grounds (SCGs) contains various bioactive compounds that have demonstrated potential applications in various fields. These compounds can enhance soil quality by improving its physicochemical properties and biological fertility, ultimately leading to improved plant growth and reducing food waste and contamination at the same time. This current study examined the impact of chemical fertilizer, vermicompost, SCGs with percentage fertilizer (SCGPF), and SCGs on the top dressing fertilizer (SCGTDF) on red radish (Raphanus sativus) growth and soil quality. This greenhouse experiment tested various concentrations of SCGPF (5%, 10%, 25%, and 50%) and different doses of SCGTDF (0.5 g, 1 g, and 2.5 g). The results showed that the 0.5 g SCGTDF treatment yielded the highest mean plant length (18.47 cm) and fresh weight (27.54 g), while the vermicompost at a 50% concentration produced the highest mean leaf surface area (58.32 cm2). These findings suggest the potential of SCGs as a sustainable fertilizer alternative, contributing to improved plant growth and soil quality, thus supporting sustainable agricultural practices and a circular economy.
Sequential Fermentation in Red Wine cv. Babić Production: The Influence of Torulaspora delbrueckii and Lachancea thermotolerans Yeasts on the Aromatic and Sensory Profile
Authors: Ivić Stipe , Jeromel Ana , Kozina Bernard , Prusina Tihomir , Budić-Leto Irena , Boban Ana , Vasilj Višnja , Jagatić Korenika Ana-Marija
Published: June 2024

This research aimed to analyze the impact of two different non-Saccharomyces yeast species on the aromatic profile of red wines made from the cv. Babić (Vitis vinifera L.) red grape variety. The grapes were obtained from two positions in the Middle and South of Dalmatia. This study compared a control treatment with the Saccharomyces cerevisiae (Sc) strain as a type of sequential inoculation treatment with Lachancea thermotolerans (Lt x Sc) and Torulaspora delbrueckii (Td x Sc). The focus was on the basic wine parameters and volatile aromatic compound concentrations determined using the SPME-Arrow-GC/MS method. The results revealed significant differences in cis-linalool oxide, geraniol, neric acid, and nerol, which contribute to the sensory profile with floral and rose-like aromas; some ethyl esters, such as ethyl furoate, ethyl hexanoate, ethyl lactate, ethyl 2-hydroxy-3-methylbutanoate, ethyl 3-hydroxy butanoate, diethyl glutarate, and diethyl succinate, contribute to the aromatic profile with fruity, buttery, overripe, or aging aromas. A sensory evaluation of wines confirmed that Td x Sc treatments exhibited particularly positive aromatic properties together with a more intense fullness, harmony, aftertaste, and overall impression.
The Nutritional Potential of Avocado By-Products: A Focus on Fatty Acid Content and Drying Processes
Authors: Marović Roko , Badanjak Sabolović Marija , Brnčić Mladen , Ninčević Grassino Antonela , Kljak Kristina , Voća Sandra , Karlović Sven , Rimac Brnčić Suzana
Published: June 2024

The aim of this study was to analyze the content of fatty acids and tocopherols in various components (pulp, seeds, peel) of avocado (Persea americana), which are often neglected as by-products. In addition, the effects of different drying processes on these components were investigated and the health benefits of the main fatty acids contained in avocados were highlighted. The samples were subjected to three drying processes: hot air (HAD), vacuum (VD), and hot-air microwave (HAMD). In all parts of fresh avocado, oleic acid was the most abundant (41.28–57.93%), followed by palmitic acid (19.90–29.45%) and linoleic acid (8.44–14.95%). Drying led to a significant reduction in the oleic acid content, with palmitic acid showing the greatest stability. HAD resulted in higher levels of oleic acid and linoleic acid in dried pulp and peel samples compared with VD and HAMD, while HAMD had the highest content of α-linolenic acid in all parts. In addition, HAMD had the shortest drying time. HAMD duration was 35 min, which was 76.7% shorter than HAD (150 min) and 82.5% shorter than VD (200 min). Considering fatty acid retention and drying efficiency, HAMD appears to have been the most effective method, especially for the avocado peel. Remarkably, the avocado peel consistently contained higher total tocopherol, with δ-tocopherol generally being the most abundant form. The high content of tocopherols, oleic acid, and linoleic acid in the avocado peel suggests promising health benefits.
Characterization of a Ligilactobacillus salivarius Strain Isolated from a Cheese Seal Which Was Last Used in 1936
Authors: Alba Claudio , Arroyo Rebeca , Fernández Leónides , Narbad Arjan , Rodríguez Juan M.
Published: June 2024

Cheesemaking played a pivotal role in the life of the Pyrenean villages where cheese was a most prized commodity and the subject of much local competition. In one of them (Sasa de Sobrepuerto), Mrs. Sebastiana Palacio decided in 1877 to label all the cheeses made in her household with a seal to differentiate them from those made by other local producers. The cheese seal was last used in 1936 and, since then, it has been kept under excellent storage conditions. Since well-preserved cheese seals are rare, and bacterial cells may survive desiccation for long periods, the objective of this work was to isolate and characterize any lactic acid bacteria that survived in the seal. Analysis of the milky crust material revealed the presence of sheep caseins. Culture-based analysis led to the isolation of a strain of Bacillus licheniformis and a strain of Ligilactobacillus salivarius (L. salivarius SP36). The latter was characterized in vitro for safety and dairy-related functional properties. Its genome encodes several genes involved in protein, peptide, and amino acid catabolism, and flavor. Overall, the phenotypic and genetic features of this strain support a high potential for being used as adjunct culture in cheesemaking.
A 3 mm Spectral Line Study of the Central Molecular Zone Infrared Dark Cloud G1.75-0.08
Authors: Miettinen Oskari , Santander-García Miguel
Published: June 2024

Infrared dark clouds (IRDCs) are fruitful objects to study the fragmentation of interstellar filaments and initial conditions and early stages of high-mass (M>8 M⊙) star formation. We used the Yebes 40 m and Institut de Radioastronomie Millimétrique (IRAM) 30 m radio telescopes to carry out the first single-pointing spectral line observations towards the IRDC G1.75-0.08, which is a filamentary Central Molecular Zone (CMZ) cloud. Our aim is to reach an improved understanding of the gas kinematics and dynamical state of the cloud and its two clumps that we call clumps A and B. We also aim to determine the fractional abundances of the molecules detected at 3 mm towards G1.75-0.08. We detected HNCO(JKa,Kc=40,4−30,3), HCN(J=1−0), and HCO+(J=1−0) towards both clumps. The N2H+(J=1−0) line was detected only in clump B, while N2D+(J=1−0) was not detected at all. The HCN and HNCO spectra exhibit two velocity components. The abundances of the detected species are comparable to those in other IRDCs. An upper limit to the [N2D+]/[N2H+] deuterium fraction of <0.05 derived towards clump B is consistent with values observed in many high-mass clumps. The line mass analysis suggests that the G1.75-0.08 filament is subcritical by a factor of 11±6, and the clumps were found to be gravitationally unbound (αvir>2). Our finding that G1.75-0.08 is strongly subcritical is atypical compared to the general population of Galactic filamentary clouds. The cloud’s location in the CMZ might affect the cloud kinematics similar to what has been found for the Brick IRDC, and the cloud’s dynamical state might also be the result of the turbulent motions or shear and tidal forces in the CMZ. Because the target clumps are dark at 70 μm and massive (several 103 M⊙), they can be considered candidates for being high-mass starless clumps but not prestellar because they are not gravitationally bound.
TNF-alfa Gene Polymorphism Associations with Multiple Sclerosis
Authors: Kalvaitis Lukas , Gedvilaite-Vaicechauskiene Greta , Kriauciuniene Loresa , Balnyte Renata , Liutkeviciene Rasa
Published: June 2024

Background: TNF-α has a dual role in multiple sclerosis (MS), contributing to both protective and harmful effects. It activates immune cells, promotes the formation of inflammatory lesions in the central nervous system, and stimulates the production of other pro-inflammatory cytokines and chemokines, leading to myelin destruction and neuronal damage. Our research focused on investigating the relationship between TNF-alpha (rs1800630, rs1800629, and rs361525) gene polymorphisms and MS. Methods: 250 healthy controls and 250 multiple sclerosis (MS) patients were included in the study. DNA was extracted from leucocytes from peripheral venous blood by salt precipitation. Single nucleotide polymorphisms (SNPs) were tested using RT–PCR. Statistical analysis of the data was performed using IBM SPSS Statistics 29.0 data analysis software. Results: The analysis revealed that the rs361525 AG genotype was significantly less frequent in the MS group compared to the control group (4.0% vs. 7.2%, p = 0.042). Sex-specific analysis showed a significant difference in genotype distribution (GG, AG, AA) among males between the MS group and the control group (97.7%, 0%, 2.3% vs. 90.6%, 9.4%, 0%, p = 0.005). For the rs1800629 polymorphism, significant results were also found. In subjects younger than 39 years, the A allele was significantly less frequent in the MS group than in the control group (8.6% vs. 15.0%, p = 0.030). The most robust model indicated that the AA genotype reduced the odds of MS by approximately 2 fold compared to the AG + GG genotype (p = 0.044), and each A allele reduced the odds of MS by approximately 2 fold (p = 0.028). The rs1800630 A allele was significantly more common in males in the MS group than in the control group (21.0% vs. 12.9%, p = 0.046). Conclusions: In conclusion, our study identifies significant associations between TNF-alpha gene variants and MS. Specifically, the rs631525 AG genotype was less common in the MS group, with notable sex-specific differences observed. The rs1800629 A allele was statistically significantly less frequent in the MS group than in the control group, and the AA genotype reduced the odds of MS occurrence by ~2 fold compared with the AG + GG genotypes. Additionally, each A allele of rs1800629 was linked to a 2-fold decreased odds of MS occurrence. In males, the rs1800630 A allele was more frequent in the MS group. These findings highlight the relevance of TNF-alpha genetic variations in MS susceptibility, suggesting potential avenues for further research and therapeutic exploration.
Impact of the Coronavirus Pandemic on Patients Requiring Tracheal Intubation by Helicopter Emergency Medical Services: A Retrospective, Single-Center, Observational Study
Authors: Hayashi Kentaro , Kikuchi Jin , Hishinuma Hidekazu , Noguchi Takafumi , Zaitsu Masayoshi , Wake Koji
Published: June 2024

Background/Objectives: The impacts of the coronavirus disease 2019 (COVID-19) pandemic on patients using helicopter emergency medical services (HEMS) regarding tracheal intubation and patient management remain unclear. Thus, we aimed to investigate this matter in Japan. Methods: In this retrospective, observational study, we analyzed 2277 patients who utilized HEMS in Tochigi Prefecture during 2018–2022. We included only patients who required tracheal intubation. We categorized patients from February 2020 to January 2022 in the pandemic group and those from February 2018 to January 2020 in the control group. We compared the interval from arrival at the scene to leaving the scene (on-scene time) and secondary variables between the two groups. Results: A total of 278 eligible patients were divided into the pandemic group (n = 127) and the control group (n = 151). The on-scene time was lower during the pandemic than that before (25.64 ± 9.19 vs. 27.83 ± 8.74 min, p = 0.043). The percentage of patients using midazolam was lower (11.8% vs. 22.5%, p = 0.02) and that of patients using rocuronium bromide was higher (29.1% vs. 6.0%, p < 0.001) during the pandemic. In contrast, the type of intervention other than tracheal intubation and the type of transportation to the hospital did not differ between the groups. Conclusions: The COVID-19 pandemic was associated with changes in the mission time of and the frequency of certain drugs administered by the HEMS. However, the type of intervention and the type of transportation did not differ. Further research is needed on changes in patient prognosis and condition due to the effects of the COVID-19 pandemic.
The Efficacy of Intraosseous Access for Initial Resuscitation in Patients with Severe Trauma: A Retrospective Multicenter Study in South Korea
Authors: Kim Youngmin , Lee Seung Hwan , Chang Sung Wook , Huh Yo , Kim Sunju , Choi Jeong Woo , Cho Hang Joo , Lee Gil Jae
Published: June 2024

Background/Objective: In patients with severe trauma, intraosseous (IO) access is an alternative when intravenous (IV) access proves challenging. However, detailed insights into its utilization patterns and effectiveness are lacking. This study aims to evaluate the use and efficacy of IO access in hemodynamically unstable patients with trauma at level-1 trauma centers in South Korea. Methods: Data from six centers over 12 months were analyzed, focusing on patients with traumatic cardiac arrest or shock. Overall, 206 patients were included in the study: 94 in the IO group and 112 in the IV group. Results: The first-attempt success rate was higher in the IO group than in the IV group (90.4% vs. 75.5%). The procedure time in the IO group was also shorter than that in the IV group. The fluid infusion rate was lower in the IO group than in the IV group; however, the use of a pressure bag with IO access significantly increased the rate, making it comparable to the IV infusion rate. Further, regarding IO access, a humeral site provided a higher infusion rate than a tibial site. Conclusions: IO access offers a viable alternative to IV access for the initial resuscitation in patients with trauma, providing advantages in terms of procedure time and first-attempt success rate. The use of a pressure bag and a humeral site for IO access afforded infusion rates comparable to those associated with IV access.
Coastal Adaptation Pathways and Tipping Points for Typical Mediterranean Beaches under Future Scenarios
Authors: Sánchez-Arcilla Agustín , Gracia Vicente , Sánchez-Arcilla Agustín
Published: June 2024

Coastal zones experience increasing climatic and human pressures, which lead to growing risks and tipping points (TPs) under future scenarios and natural resource scarcity. To avoid crossing TPs with irreversible coastal losses, this paper proposes the development of adaptation pathways based on advanced coastal oceanography and engineering knowledge that enables a comprehensive assessment of evolving coastal risks. These pathways feature sequential interventions steered by simulations and observations as a function of available coastal resources (mainly space and sediment) and risks for infrastructure and socioeconomic assets. Such an adaptation has been developed for urban and peri-urban Mediterranean beaches, considering conventional coastal engineering together with nature-based solutions (NbS). Both types of interventions are assessed in terms of key physical variables, which serve to evaluate performance and estimate TPs. This analysis supports the new coastal protection and management plan promoted by the regional government of Catalonia and the coastal adaptation plan of the central government of Spain. The approach and results illustrate the potential of adaptation pathways for beach sustainability, enhancing the compatibility between short-/long-term coastal protection objectives under present/future climate and management scenarios. The development of adaptation pathways underpins increasing stakeholder cooperation to achieve shared decisions for coastal sustainability.
Cross-Scale Modeling of Shallow Water Flows in Coastal Areas with an Improved Local Time-Stepping Method
Authors: Liu Guilin , Ji Tao , Wu Guoxiang , Tian Hao , Yu Pubing
Published: June 2024

A shallow water equations-based model with an improved local time-stepping (LTS) scheme is developed for modeling coastal hydrodynamics across multiple scales, from large areas to detailed local regions. To enhance the stability of the shallow water model for long-duration simulations and at larger LTS gradings, a prediction-correction method using a single-layer interface that couples coarse and fine time discretizations is adopted. The proposed scheme improves computational efficiency with an acceptable additional computational burden and ensures accurate conservation of time truncation errors in a discrete sense. The model performance is verified with respect to conservation and computational efficiency through two idealized tests: the spreading of a drop of shallow water and a tidal flat/channel system. The results of both tests demonstrate that the improved LTS scheme maintains precision as the LTS grading increases, preserves conservation properties, and significantly improves computational efficiency with a speedup ratio of up to 2.615. Furthermore, we applied the LTS scheme to simulate tides at grid scales of 40,000 m to 200 m for a portion of the Northwest Pacific. The proposed model shows promise for modeling cross-scale hydrodynamics in complex coastal and ocean engineering problems.
Experimental Study on Cyclic Simple Shear Test of Coastal Tidal Soft Soil
Authors: Yu Xiuxia , Sun Honglei , Liu Junwei , Zhao Hongxuan , Han Yu
Published: June 2024

Based on undrained cyclic simple shear tests conducted on coastal tidal soft soil under various conditions of cyclic stress ratios and moisture contents, this study investigated the influence of these factors on the dynamic properties of the soil. The findings indicated that with increasing moisture content and stress cycle ratio, the stress–strain hysteresis loop gradually expanded, resulting in a higher strain difference and a transition from a dense to a sparse curve pattern. Moreover, the symmetry of the hysteresis loop was lost in the later stages of shearing. With an increase in the number of cycles, the cumulative shear strain gradually increased, and the increase in the cyclic ratio of water content to stress reduced the number of cyclic shear cycles required to achieve failure, thereby accelerating the soil’s failure rate. A predictive formula was developed based on the experimental results to estimate the failure cycles as a function of the cyclic stress ratio and moisture content. Furthermore, the softening index decreased gradually with an increasing number of cycles, and a higher moisture content and cyclic stress ratio accelerated the soil’s softening process. It was observed that under the conditions of optimal moisture content, the soil exhibited a slower softening rate during the initial stage of shearing.
Movement Behavior of the Dusky Grouper Epinephelus marginatus (Lowe, 1834) in Early Life Stages
Authors: Goçalo Cássia Gongora , Lopes Rubens M.
Published: June 2024

The dusky grouper (Epinephelus marginatus) is a vulnerable predatory fish found in the Atlantic and Indian oceans, and in the Mediterranean Sea. This study investigates the movement patterns of grouper larvae (151 individuals) during their first feeding period (three to ten days post hatching) through laboratory experiments offering rotifers (Brachionus sp.) and copepod nauplii as food. High-speed cameras and imaging techniques including bright field microscopy, matched filters, and holography captured rapid displacements (up to 25 body lengths per second), peduncle caudal beats (up to 40 beats s−1), turns, and resting periods. Reynolds numbers ranged from <45 for routine swimming to 222 for burst swimming. Specific behaviors, including changes in swimming velocity and body movements, were observed from three days post hatching, when feeding begins, suggestive of an array of responses to environmental forcing, predator avoidance, food search, and feeding success. These findings provide insights into the critical movement performances of E. marginatus larvae, which help to unravel their ecological interactions and survival strategies. Understanding grouper larval swimming behavior is pivotal for devising effective cultivation strategies aimed at replenishing wild stocks and enhancing production for human consumption.
Analysis of Supply Chain Response Frameworks: A Literature Review
Authors: Díaz Pacheco Raúl Antonio , Benedito Ernest
Published: June 2024

Background: Various supply chain response frameworks (SCRFs) have been proposed in the supply chain (SC) literature, but there is no in-depth analysis. This study analyzes the applicability of SCRFs in scenarios that require SC responses by examining the frameworks’ design and use in response situations. Methods: A qualitative analysis of 38 studies revealed weaknesses in SCRFs, which include the entity proposing the framework, the stimulus being responded to, the adaptation of activities to the stimulus that is responded to, objectives, and response evaluation criteria. Results: The analysis reveals that while these frameworks have been designed for specific situations involving single SC processes, they demonstrate weaknesses by failing to meet two requirements: (1) the stimulus being responded to is different from changes in demand, and (2) the response is generated by a process distinct from manufacturing. Conclusions: Further, SCRF research that incorporates these weaknesses will promote the fragmented development of the SCR concept. Conversely, a robust SCRF can be successfully utilized in various SCRs, facilitating the comparison and evaluation of responses of different SCs to the same stimulus.
Modeling Residential Energy Consumption Patterns with Machine Learning Methods Based on a Case Study in Brazil
Authors: Henriques Lucas , Castro Cecilia , Prata Felipe , Leiva Víctor , Venegas René
Published: June 2024

Developing efficient energy conservation and strategies is relevant in the context of climate change and rising energy demands. The objective of this study is to model and predict the electrical power consumption patterns in Brazilian households, considering the thresholds for energy use. Our methodology utilizes advanced machine learning methods, such as agglomerative hierarchical clustering, k-means clustering, and self-organizing maps, to identify such patterns. Gradient boosting, chosen for its robustness and accuracy, is used as a benchmark to evaluate the performance of these methods. Our methodology reveals consumption patterns from the perspectives of both users and energy providers, assessing the corresponding effectiveness according to stakeholder needs. Consequently, the methodology provides a comprehensive empirical framework that supports strategic decision making in the management of energy consumption. Our findings demonstrate that k-means clustering outperforms other methods, offering a more precise classification of consumption patterns. This finding aids in the development of targeted energy policies and enhances resource management strategies. The present research shows the applicability of advanced analytical methods in specific contexts, showing their potential to shape future energy policies and practices.
Big Data Privacy Protection and Security Provisions of the Healthcare SecPri-BGMPOP Method in a Cloud Environment
Authors: Kuttiyappan Moorthi , Appadurai Jothi Prabha , Kavin Balasubramanian Prabhu , Selvaraj Jeeva , Gan Hong-Seng , Lai Wen-Cheng
Published: June 2024

One of the industries with the fastest rate of growth is healthcare, and this industry’s enormous amount of data requires extensive cloud storage. The cloud may offer some protection, but there is no assurance that data owners can rely on it for refuge and privacy amenities. Therefore, it is essential to offer security and privacy protection. However, maintaining privacy and security in an untrusted green cloud environment is difficult, so the data owner should have complete data control. A new work, SecPri-BGMPOP (Security and Privacy of BoostGraph Convolutional Network-Pinpointing-Optimization Performance), is suggested that can offer a solution that involves several different steps in order to handle the numerous problems relating to security and protecting privacy. The Boost Graph Convolutional Network Clustering (BGCNC) algorithm, which reduces computational complexity in terms of time and memory measurements, was first applied to the input dataset to begin the clustering process. Second, it was enlarged by employing a piece of the magnifying bit string to generate a safe key; pinpointing-based encryption avoids amplifying leakage even if a rival or attacker decrypts the key or asymmetric encryption. Finally, to determine the accuracy of the method, an optimal key was created using a meta-heuristic algorithmic framework called Hybrid Fragment Horde Bland Lobo Optimisation (HFHBLO). Our proposed method is currently kept in a cloud environment, allowing analytics users to utilise it without risking their privacy or security.
Intraoperative Dexmedetomidine Use for Enhanced Recovery after Surgery (ERAS) in Cardiac Surgery—Single Center Retrospective Observational Cohort Study
Authors: Kerroum Axel , Rosner Lorenzo , Scala Emmanuelle , Kirsch Matthias , Tozzi Piergiorgio , Courbon Cécile , Rusca Marco , Abramavičius Silvijus , Andrijauskas Povilas , Marcucci Carlo , Rancati Valentina
Published: June 2024

Background and Objectives: Dexmedetomidine, an alpha-2 agonist, is used as an adjunct to anesthesia in enhanced recovery after surgery (ERAS) programs. One of its advantages is the opioid-sparing effect which can facilitate early extubation and recovery. When the ERAS cardiac society was set in 2017, our facility was already using the ERAS program, in which the “fast-track Anesthesia” was facilitated by the intraoperative infusion of dexmedetomidine. Our objective is to share our experience and investigate the potential impact of intraoperative dexmedetomidine use as a part of the ERAS program on patient outcomes in elective cardiac surgery. Materials and Methods: An observational retrospective cohort study was conducted at a university hospital in Switzerland. The patients who underwent elective cardiac surgery with cardiopulmonary bypass between 1 June 2017 and 31 August 2018 were included in this analysis (n = 327). Regardless of the surgery type, all the patients received a standardized fast-track anesthesia protocol inclusive of dexmedetomidine infusion, reduced opioid dose, and parasternal nerve block. The primary outcome was the postoperative time when the criteria for extubation were met. Three groups were identified: group 0—(extubated in the operating room), group < 6 (extubated in less than 6 h), and group > 6 (extubated in >6 h). The secondary outcomes were adverse events, length of stay in ICU and in hospital, and total hospitalization costs. Results: Dexmedetomidine was well-tolerated, with no significant adverse events reported. Early extubation was performed in 187 patients (57%). Group 3 had a significantly longer length of stay in the ICU (median: 70 h vs. 25 h) and in hospital (17 vs. 12 days), and consequently higher total hospitalization costs (CHF 62,551 vs. 38,433) compared to the net data from the other two groups (p < 0.0001). Conclusions: Our findings suggest that dexmedetomidine can be safely used as part of the opioid-sparing anesthesia protocol in patients undergoing elective cardiac surgery with cardiopulmonary bypass with the potential to facilitate early extubation, shorter ICU and hospital stays, and reduced hospitalization costs.
Updates on Antibiotic Regimens in Acute Cholecystitis
Authors: Fico Valeria , La Greca Antonio , Tropeano Giuseppe , Di Grezia Marta , Chiarello Maria Michela , Brisinda Giuseppe , Sganga Gabriele
Published: June 2024

Acute cholecystitis is one of the most common surgical diseases, which may progress from mild to severe cases. When combined with bacteremia, the mortality rate of acute cholecystitis reaches up to 10–20%. The standard of care in patients with acute cholecystitis is early laparoscopic cholecystectomy. Percutaneous cholecystostomy or endoscopic procedures are alternative treatments in selective cases. Nevertheless, antibiotic therapy plays a key role in preventing surgical complications and limiting the systemic inflammatory response, especially in patients with moderate to severe cholecystitis. Patients with acute cholecystitis have a bile bacterial colonization rate of 35–60%. The most frequently isolated microorganisms are Escherichia coli, Klebsiella spp., Streptococcus spp., Enterococcus spp., and Clostridium spp. Early empirical antimicrobial therapy along with source control of infection is the cornerstone for a successful treatment. In these cases, the choice of antibiotic must be made considering some factors (e.g., the severity of the clinical manifestations, the onset of the infection if acquired in hospital or in the community, the penetration of the drug into the bile, and any drug resistance). Furthermore, therapy must be modified based on bile cultures in cases of severe cholecystitis. Antibiotic stewardship is the key to the correct management of bile-related infections. It is necessary to be aware of the appropriate therapeutic scheme and its precise duration. The appropriate use of antibiotic agents is crucial and should be integrated into good clinical practice and standards of care.
Quest for the Nitrogen-Metabolic Versatility of Microorganisms in Soil and Marine Ecosystems
Authors: Zhao Yongpeng , Zhu-Barker Xia , Cai Kai , Wang Shuling , Wright Alan L. , Jiang Xianjun
Published: June 2024

Whether nitrogen (N)-metabolic versatility is a common trait of N-transforming microbes or if it only occurs in a few species is still unknown. We collected 83 soil samples from six soil types across China, retrieved 19 publicly available metagenomic marine sample data, and analyzed the functional traits of N-transforming microorganisms using metagenomic sequencing. More than 38% and 35% of N-transforming species in soil and marine ecosystems, respectively, encoded two or more N-pathways, although N-transforming species differed greatly between them. Furthermore, in both soil and marine ecosystems, more than 80% of nitrifying and N-fixing microorganisms at the species level were N-metabolic versatile. This study reveals that N-metabolic versatility is a common trait of N-transforming microbes, which could expand our understanding of the functional traits of drivers of nitrogen biogeochemistry.
Role of Nedd4L in Macrophage Pro-Inflammatory Polarization Induced by Influenza A Virus and Lipopolysaccharide Stimulation
Authors: Peng Meihong , Zhao Cheng , Lu Fangguo , Zhang Xianggang , Wang Xiaoqi , He Li , Chen Bei
Published: June 2024

Influenza A virus (IAV) infection often leads to influenza-associated fatalities, frequently compounded by subsequent bacterial infections, particularly Gram-negative bacterial co-infections. Lipopolysaccharide (LPS), a primary virulence factor in Gram-negative bacteria, plays a crucial role in influenza–bacterial co-infections. However, the precise pathogenic mechanisms underlying the synergistic effects of viral–bacterial co-infections remain elusive, posing significant challenges for disease management. In our study, we administered a combination of IAV and LPS to mice and examined associated parameters, including the lung function, lung index, wet/dry ratio, serum inflammatory cytokines, Nedd4L expression in lung tissue, and mRNA levels of inflammatory cytokines. Co-infection with IAV and LPS exacerbated lung tissue inflammation and amplified M1 macrophage expression in lung tissue. Additionally, we stimulated macrophages with IAV and LPS in vitro, assessing the inflammatory cytokine content in the cell supernatant and cytokine mRNA expression within the cells. This combined stimulation intensified the inflammatory response in macrophages and upregulated Nedd4L protein and mRNA expression. Subsequently, we used siRNA to knockdown Nedd4L in macrophages, revealing that suppression of Nedd4L expression alleviated the inflammatory response triggered by concurrent IAV and LPS stimulation. Collectively, these results highlight the pivotal role of Nedd4L in mediating the exacerbated inflammatory responses observed in IAV and LPS co-infections.