bluuebunny/crossref_metadata_2025 huggingface dataset:
Growth, Cell Division, and Gene Expression of Escherichia coli at Elevated Concentrations of Magnesium Sulfate: Implications for Habitability of Europa and Mars
Authors: Nepal Sudip , Kumar Pradeep
Published: April 2020

We perform quantitative studies of the growth, death, and gene expression of Escherichia coli in a wide range of magnesium sulfate (MgSO 4 ) concentrations (0–2.5 M). Elevated concentration of MgSO 4 causes the inhibition of cell growth, leading to an increase in the population doubling time. We find that cells exhibit three distinct morphological phenotypes—(i) normal, (ii) filamentous, and (iii) small cells at 1.25 M MgSO 4 . Filamentous cells arise due to the lack of cell division, while the small cells arise due to the partial plasmolysis of the cells. We further find that cell death starts for salt concentrations >1.25 M and increases with an increasing concentration of MgSO 4 . For salt concentrations ≥1.66 M, the growth of cells stops and all the cells become smaller than the control cells, suggesting the plasmolysis of the population. Cells grown at salt concentration up to 2.07 M are reversible in both the growth rate and morphology upon the removal of the salt stress. The time scale of reversibility increases with increasing salt concentration. Finally, we investigate the expression of an osmotically inducible gene (osmC), genes involved in magnesium transport (corA), sulfate transport (cysP), and osmotically driven transport of water (aqpZ). We find that a high concentration of magnesium sulfate leads to the upregulation of cysP and osmC.
Microstructural Investigations on Plasticity of Lime-Treated Soils
Authors: Vitale Enza , Deneele Dimitri , Russo Giacomo
Published: April 2020

The surface charge distribution of clay particles governs the interparticle forces and their arrangement in clay-water systems. The plasticity properties are the consequences of the interaction at the microscopic scale, even if they are traditionally linked to the mechanical properties of fine-grained soils. In the paper, the plasticity modifications induced by the addition of lime were experimentally investigated for two different clays (namely kaolinite and bentonite) in order to gain microstructural insights of the mechanisms affecting their plastic behavior as a function of the lime content and curing time. Zeta potential and dynamic light scattering measurements, as well as thermogravimetric analyses, highlighted the mechanisms responsible for the plastic changes at a small scale. The increase of the interparticle attraction forces due to the addition of lime increased the liquid and plastic limits of kaolinite in the short term, without significant changes in the long term due to the low reactivity of the clay in terms of pozzolanic reactions. The addition of lime to bentonite resulted in a decrease of interparticle repulsion double layer interactions. Rearrangement of the clay particles determined a reduction of the liquid limit and an increase of the plastic limit of the treated clays in the very short term. Precipitation of the bonding compounds due to pozzolanic reactions increased both the liquid and plastic limits over the time.
Mineralogical and Geochemical Characterization of Talc from Two Mexican Ore Deposits (Oaxaca and Puebla) and Nine Talcs Marketed in Mexico: Evaluation of Its Cosmetic Uses
Authors: Pi-Puig Teresa , Animas-Torices Dante Yosafat , Solé Jesús
Published: April 2020

The detailed mineralogical, physical and chemical characterization of nine samples of imported cosmetic talc and of two samples of talc from currently non-productive Mexican ore deposits (Oaxaca and Puebla States) is presented. The imported cosmetic talc was classified into two groups, considering whether they are packed in the country of origin or in Mexico and considering their price. X-ray diffraction, infrared short wave, thermogravimetric analysis and scanning electron microscopy were used for mineralogical characterization. For the physical characterization, colorimetry and laser granulometry were used. The chemical composition (major, trace elements) was studied by ICP-MS. It was concluded that only the highest priced and imported in packaged form talcs meet the specific purity requirements for a talc of cosmetic type. The talcs that are packed in Mexico and the talc of the studied Mexican deposits present mineralogical and chemical impurities that make their use difficult in the manufacture of high-quality cosmetic talc. The low-price talc should not be sold as cosmetic talc, and the regulations in Mexico on this subject should be reviewed and updated.
Volcanic Grinding Tools in Ustica Island (Tyrrhenian Sea, Italy): Local Production vs. Import of Morgantina-Type Millstones in the Hellenistic-Roman Period
Authors: Santi Patrizia , Foresta Martin Franco , Spatafora Francesca , de Vita Sandro , Renzulli Alberto
Published: April 2020

This archaeometric study was focused on 28 grey to dark-grey lava artifacts found in Ustica Island (Southern Tyrrhenian Sea, Italy) and referable to different grinding tools: saddle querns, rotary Morgantina-type millstones, rotary hand-mills and one small mortar. Mineralogy, petrography and bulk rock geochemical analyses emphasized that most of the grinding artifacts belonged to the Na-Alkaline series of Ustica, mainly basalts, hawaiites and mugearites. Nevertheless, some millstone samples did not match major and trace elements of Ustica lavas, in particular, one high-TiO2 Na-Alkaline basalt from Pantelleria Island, some tholeiitic/transitional basalts from the Iblei Mountains and one Calcalkaline basaltic andesite, most likely from the Aeolian Archipelago. The Hellenistic–Roman re-colonisation of Ustica Island, after ca. one millennium of nearly complete abandonment, was testified by the import of the non-local Morgantina-type rotary millstones, very widespread in the Mediterranean area from 4th–3rd century BC. This import of millstones represented, for the Ustica inhabitants, a real breakthrough for developing a local production of grinding artifacts on the basis of the new rotary technique which was much more efficient than that of the archaic saddle querns, largely used in the Middle Bronze Age. The results are also discussed in the framework of the overall volcanic millstone trade in the Mediterranean area and the different milling technology in antiquity.
Metal-Containing Zinc Phosphate EDI Zeolites Synthesized by Sol–gel Assisted Hydrothermal Method
Authors: Wang Xuelei , Dong Zhaojun , Wang Qiufeng , Meng Chao , Zhuang Weibin , Liu Jiyuan , Song Ying , Jin Yuxin , Yang Shaobin
Published: April 2020

Three different metal-containing zinc phosphate, [C3H12N2][Zn0.5Fe1.5(PO4)2] (1), [C3H12N2][Zn0.67Co1.33(PO4)2] (2) and [C3H12N2][Zn0.67Ni1.33(PO4)2] (3) with EDI topology were prepared by sol–gel assisted hydrothermal method. The advantages of this method are lower synthesis temperature and uniform mixing. The crystalline metal-containing zinc phosphate zeolites exhibit a variety of SEM morphologies because of the entanglement of three different metal ions. The zinc ions in the zinc phosphate EDI molecular sieve were partially substituted by Fe, Co and Ni ions. The ICP analysis shows that the metal ratios of Zn/M are 1/3, 1/2 and 1/2. Variable temperature susceptibility was measured on powder samples in the range 2–300 K. All three M-EDI molecular sieves exhibit antiferromagnetic properties. In addition, they were analyzed by XRD, CHN, IR and TG.
Allanite Geochemical Response to Hydrothermal Alteration by Alkaline, Low-Temperature Fluids
Authors: Gros Katarzyna , Słaby Ewa , Jokubauskas Petras , Sláma Jiří , Kozub-Budzyń Gabriela
Published: April 2020

Allanite is one of the main rare earth elements (REE)-rich accessory minerals in composite dykes from the granitoid pluton of Karkonosze. These dykes differ in composition from the bulk of the pluton by elevated rare earth elements (REE), Y, Zr, and alkali contents, suggesting contribution of an additional component. Allanite exhibits complex alteration textures, which can be divided into two stages. The first stage is represented by allanite mantles, formed by fluid infiltration into previously crystallized magmatic allanite. These zones have low totals, are Ca-, Al-, Mg-, and light REE (LREE)-depleted, and Y-, heavy REE (HREE)-, Th-, Ti-, and alkali-enriched. The fractionation between LREE and HREE was caused by different mobility of complexes formed by these elements in aqueous fluids. The second stage includes recrystallized LREE-poor, Y-HREE-rich allanite with variable Ca, Al, Mg, and REE-fluorocarbonates. The alteration products from both stages demonstrate higher Fe3+/(Fe2+ + Fe3+) ratios and a negative Ce anomaly. These features point to the alkaline, low-temperature, and oxidized nature of the fluids. The differences in mobility and solubility of respective ligands show that the fluids from the first stage may have been dominated by Cl, whereas those of the second stage may have been dominated by F and CO2 (and PO4 in case of one sample). The inferred chemistry of the fluids resembles the overall geochemical signature of the composite dykes, indicating a major contribution of the hydrothermal processes to their geochemical evolution.
Analysis of GPI-Anchored Receptor Distribution and Dynamics in Live Cells by Tag-Mediated Enzymatic Labeling and FRET
Authors: Balatskaya Maria N. , Baglay Alexandra I. , Rubtsov Yury P. , Sharonov George V.
Published: April 2020

The analysis of glycosylphosphatidylinositol (GPI)-anchored receptor distribution and dynamics in live cells is challenging, because their clusters exhibit subdiffraction-limited sizes and are highly dynamic. However, the cellular response depends on the GPI-anchored receptor clusters’ distribution and dynamics. Here, we compare three approaches to GPI-anchored receptor labeling (with antibodies, fluorescent proteins, and enzymatically modified small peptide tags) and use several variants of Förster resonance energy transfer (FRET) detection by confocal microscopy and flow cytometry in order to obtain insight into the distribution and the ligand-induced dynamics of GPI-anchored receptors. We found that the enzyme-mediated site-specific fluorescence labeling of T-cadherin modified with a short peptide tag (12 residues in length) have several advantages over labeling by fluorescent proteins or antibodies, including (i) the minimized distortion of the protein’s properties, (ii) the possibility to use a cell-impermeable fluorescent substrate that allows for selective labeling of surface-exposed proteins in live cells, and (iii) superior control of the donor to acceptor molar ratio. We successfully detected the FRET of GPI-anchored receptors, T-cadherin, and ephrin-A1, without ligands, and showed in real time that adiponectin induces stable T-cadherin cluster formation. In this paper (which is complementary to our recent research (Balatskaya et al., 2019)), we present the practical aspects of labeling and the heteroFRET measurements of GPI-anchored receptors to study their dynamics on a plasma membrane in live cells.
Structural, Electronic and Magnetic Properties of a Few Nanometer-Thick Superconducting NdBa2Cu3O7 Films
Authors: Moretti Sala Marco , Salluzzo Marco , Minola Matteo , De Luca Gabriella Maria , Dellea Greta , Srot Vesna , Wang Yi , van Aken Peter A. , Le Tacon Matthieu , Keimer Bernhard , Dallera Claudia , Braicovich Lucio , Ghiringhelli Giacomo
Published: April 2020

Epitaxial films of high critical temperature ( T c ) cuprate superconductors preserve their transport properties even when their thickness is reduced to a few nanometers. However, when approaching the single crystalline unit cell (u.c.) of thickness, T c decreases and eventually, superconductivity is lost. Strain originating from the mismatch with the substrate, electronic reconstruction at the interface and alteration of the chemical composition and of doping can be the cause of such changes. Here, we use resonant inelastic x-ray scattering at the Cu L 3 edge to study the crystal field and spin excitations of NdBa 2 Cu 3 O 7 − x ultrathin films grown on SrTiO 3 , comparing 1, 2 and 80 u.c.-thick samples. We find that even at extremely low thicknesses, the strength of the in-plane superexchange interaction is mostly preserved, with just a slight decrease in the 1 u.c. with respect to the 80 u.c.-thick sample. We also observe spectroscopic signatures for a decrease of the hole-doping at low thickness, consistent with the expansion of the c-axis lattice parameter and oxygen deficiency in the chains of the first unit cell, determined by high-resolution transmission microscopy and x-ray diffraction.
Carrier Transfer and Capture Kinetics of the TiO2/Ag2V4O11 Photocatalyst
Authors: Zhou Yun , Ding Qiujie , Wang Yuan , OuYang Xiaoping , Liu Lixin , Li Junyu , Wang Bing
Published: April 2020

In this paper, TiO2/Ag2V4O11 nanoheterojunctions have been synthesized by hydrothermal methods, which show enhanced photocatalytic activity compared to TiO2 under visible light. Moreover, the TiO2/Ag2V4O11 nanoheterojunction with set molar ratio of 2:1, referred to as TA2, show the highest visible light photocatalytic activity, which could decompose about 100% RhB molecules within 80 min of irradiation with visible light. Specially, the time-resolved photoluminescence spectrum of TA2 demonstrates that the free exciton recombination occurs in approximately 1.7 ns, and the time scale for Shockley–Read–Hall recombination of photogenerated electrons and holes is prolonged to 6.84 ns. The prolonged timescale of TA2 compared to TiO2 and Ag2V4O11 can be attributed to the carrier separation between nanojunctions and the carrier capture by interfacial defects. Furthermore, the enhanced photocatalytic activity of TiO2/Ag2V4O11 nanoheterojunctions also benefits from the synergistic effect of the broadened absorption region, higher photocarrier generation, longer carrier lifetime, and quicker collection dynamics.
Comparative Study of Three Carbon Additives: Carbon Nanotubes, Graphene, and Fullerene-C60, for Synthesizing Enhanced Polymer Nanocomposites
Authors: Wang Xingyu , Tang Fujian , Cao Qi , Qi Xiaoning , Pearson Matthew , Li Mingli , Pan Hong , Zhang Zi , Lin Zhibin
Published: April 2020

While nanoparticles from the carbon family have been incorporated effectively for polymer matrixes, there is no clear information available for understanding the impacts of the morphology of different carbon nanoparticles on the performance of carbon-based nanocomposites. Therefore, this study aimed to provide a comprehensive, comparative investigation to systematically assess the impacts of nanoparticles on the tribological, mechanical, and electrochemical properties of the epoxy coatings using three representative 0D, 1D, and 2D nanoparticles: Fullerene-C60 (C60), graphene nanoplatelets (GNPs), and carbon nanotubes (CNTs). The anti-corrosion performance of the nanocomposites in both the short and long term was characterized. The mechanical properties were examined by abrasion, adhesion, and tensile tests. Fourier-transform infrared spectroscopy (FTIR) was conducted to determine their chemical structures, while scanning electron microscopy (SEM) was used to determine their surface texture. The electrochemical impedance spectroscopy (EIS) results revealed that the coatings reinforced by C60 and GNP had better anti-corrosion performance than that of the CNT/epoxy samples. The incorporation of C60 and CNT led to a considerable improvement in tensile properties, while improved abrasion resistance was observed in all types of nanofiller/epoxy groups. C60-loaded composites exhibited a significant enhancement in tensile properties as compared to CNT or GNP composites.
Low-Terahertz Transparent Graphene-Based Absorber
Authors: D’Aloia Alessandro Giuseppe , D’Amore Marcello , Sarto Maria Sabrina
Published: April 2020

A new, transparent, metal-free absorber, based on the use of multilayer graphene/dielectric laminates (GLs), is proposed for applications in the low-terahertz frequency range. The designed absorber has a total thickness of around 70 µm and consists of a front matching dielectric layer followed by a GL, a dielectric spacer and a back GL. The laminates are periodic structures constituted of graphene sheets separated by 50-nm-thick polyethylene terephthalate (PET) interlayers, while the matching layer and the spacer are one-quarter-wavelength thick and made of PET. The GLs are modeled as homogeneous-equivalent single layers (ESLs) characterized by their sheet resistances Rs. An innovative analytical method is proposed in order to select Rs values optimizing the electromagnetic wave absorption either in low-gigahertz or low-terahertz frequency range. The frequency spectra of the absorption, reflection and transmission coefficients are computed in the range up to 4 THz by using different values of Rs. Then, realistic Rs values of chemically doped graphene monolayers over PET substrates are considered. The designed absorbers are characterized by an absorption coefficient with a peak value of about 0.8 at the first resonant frequency of 1.1 THz, and a 1.4 THz bandwidth centered at 1.5 THz with reflection coefficient below - 10 dB. Moreover, the optical transmittance of the proposed absorbers are computed by means of the optical matrix theory and it is found to be greater than 86% in all the visible ranges.
Nanoemulgel for Improved Topical Delivery of Retinyl Palmitate: Formulation Design and Stability Evaluation
Authors: Algahtani Mohammed S. , Ahmad Mohammad Zaki , Ahmad Javed
Published: April 2020

Retinyl palmitate is a vitamin A ester belonging to the family of endogenous natural retinoid and used to treat various skin disorders like acne, skin aging, wrinkles, and dark spots, as well as to protect against psoriasis. Despite the known therapeutic benefits of retinyl palmitate, the conventional topical delivery of retinyl palmitate commonly associated with adverse reactions such as skin irritation, redness, excessive peeling, and dryness. Therefore, the current study aims to encapsulate the retinyl palmitate in nanoemulsion then incorporate it into a hydrogel system to improve the topical delivery and stability. Low-energy emulsification method was used for the nano-encapsulation of retinyl palmitate. The phase behavior study was used for the investigation and the optimization of the formulation. The droplet size of the optimized nanoemulsion was in nano dimension (16.71 nm) with low polydispersity index (PdI) (0.015), negative zeta potential (−20.6 mV). It demonstrated the influence of vortexing on droplet size and PdI during nanoemulsion preparation. The retinyl palmitate loaded nanoemulgel delivery system exhibited significant improvement (p < 0.05) in skin permeability after topical application. Employment of the nano-encapsulation approach afterward dispersion into hydrogel system for the development of a topical delivery system of retinyl palmitate resulted in improvement in its UV and storage stability as well.
The Development of Cooking Videos to Encourage Calcium Intake in Young Adults
Authors: Bramston Vienna , Rouf Anika , Allman-Farinelli Margaret
Published: April 2020

Young adults are among the lowest consumers of calcium-rich foods. As young adults move out of home and commence university, meal skipping, food budgets and poor cooking skills may contribute to low intakes. This research aimed to develop and evaluate cooking videos to educate young adults about calcium-containing foods and provide demonstrations for culinary skills training. Fifteen short videos were designed that required minimal cooking skills, ingredients of low cost, and covered main meals and snacks. Thirty-four young adults (nine males) participated in four focus groups to assess usability and desirability of content and explore barriers to cooking. Individually completed questionnaires assessed knowledge and motivation gained post-video screening. Qualitative data were analysed with both a deductive and inductive thematic approach, and questionnaires using descriptive statistics. Video content was well accepted, most participants reported knowledge was gained and their motivation to prepare food at home and consume calcium-rich foods increased. Cooking videos appear to be a well-accepted alternative to formal classes to demonstrate calcium-rich meals that can be quickly prepared. In the future, the videos should be tested in a trial of effectiveness as social media presents a dissemination opportunity for these videos among university and college students.
Topical Application of A New Herbal Complex, NI-01, Ameliorates House Dust Mite-Induced Atopic Dermatitis in NC/Nga Mice
Authors: Jin Seong Eun , Ha Hyekyung , Yoo Sae-Rom , Jeon Woo-Young , Lee Nari , Lee Mee-Young , Choi Susanna , Jang Ji-Hye , Park Eunsook , Kim Sukkyoung , Seo Chang-Seob
Published: April 2020

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritus and cutaneous dry skin. Here, we investigated whether topical application of NI-01 composed of six herbal medicines has a therapeutic effect on AD in vivo. Twelve marker compounds of NI-01 were analyzed by high-performance liquid chromatography with a photodiode array detector for quality control. To induce AD, house dust mite extract was applied to the shaved dorsal skin and ear surfaces of NC/Nga mice twice a week for 6 weeks. NI-01 (1, 2, or 4 mg/mouse) was applied daily to the site for experiment periods. The coefficient of determination of each compound showed good linearity (≥ 0.9999). The recovery rate of the 12 marker components was 96.77%–105.17%; intra and interday precision and repeatability were ≤ 1.40%. Topical application of NI-01 reduced house dust mite induced AD symptoms. The increased expressions of interleukin-4 and intercellular adhesion molecule-1 caused by house dust mites were markedly suppressed in NI-01-treated mice. Corticosterone levels significantly decreased, whereas serotonin levels increased with NI-01 application. These results suggest that NI-01 alleviates AD symptoms by inhibiting infiltration of inflammatory cells, thereby decreasing AD-related stress. NI-01 could be beneficial for the treatment of AD-like skin diseases.
Anti-Obesity Effects of Tanshinone I from Salvia miltiorrhiza Bunge in Mice Fed a High-Fat Diet through Inhibition of Early Adipogenesis
Authors: Jung Dae Young , Kim Ji-Hyun , Jung Myeong Ho
Published: April 2020

Tanshinone I (Tan I) is a diterpenoid isolated from Salvia miltiorrhiza Bunge and exhibits antitumor effects in several cancers. However, the anti-obesity properties of Tan I remain unexplored. Here, we evaluated the anti-obesity effects of Tan I in high-fat-diet (HFD)-induced obese mice and investigated the underlying molecular mechanisms in 3T3-L1 cells. HFD-induced obese mice were orally administrated Tan I for eight weeks, and body weight, weight gain, hematoxylin and eosin staining and serum biological parameters were examined. The adipogenesis of 3T3-L1 preadipocytes was assessed using Oil Red O staining and measurement of intracellular triglyceride (TG) levels, and mitotic clonal expansion (MCE) and its related signal molecules were analyzed during early adipogenesis of 3T3-L1 cells. The administration of Tan I significantly reduced body weight, weight gain, and white adipocyte size, and improved obesity-induced serum levels of glucose, free fatty acid, total TG, and total cholesterol in vivo in HFD-induced obese mice. Furthermore, Tan I-administered mice demonstrated improvement of glucose metabolism and insulin sensitivity. Treatment with Tan I inhibited the adipogenesis of 3T3-L1 preadipocytes in vitro, with this inhibition mainly occurring at an early phase of adipogenesis through the attenuation of MCE via cell cycle arrest at the G1/S phase transition. Tan I inhibited the phosphorylation of p38, extracellular signal-regulated kinase (ERK), and Akt during the process of MCE, while it stimulated the phosphorylation of AMP-activated protein kinase. Furthermore, Tan I repressed the expression of CCAAT-enhancer-binding protein β (C/EBPβ), histone H3K9 demethylase JMJD2B, and subsequently cell cycle genes. Moreover, Tan I regulated the expression of early adipogenic transcription factors including GATAs and Kruppel-like factor family factors. These results indicate that Tan I prevents HFD-induced obesity via the inhibition of early adipogenesis, and thus improves glucose metabolism and insulin sensitivity. This suggests that Tan I possesses therapeutic potential for the treatment of obesity and obesity-related diseases.
Escherichia coli Strains with Virulent Factors Typical for Uropathogens were Isolated from Sinuses from Patients with Chronic Rhinosinusitis—Case Report
Authors: Krawczyk Beata , Michalik Michał , Fordon Magdalena , Wysocka Magdalena , Samet Alfred , Nowicki Bogdan
Published: April 2020

Escherichia coli were isolated from three patients with chronic rhinosinusitis (CRS) by intraoperative sinus tissue biopsy. Taking into account the unusual replicative niche and previous treatment failures, it was decided to focus on the virulence and drug resistance of these bacteria. The strains turned out to be multi-sensitive, but the rich virulence factors profile of bacteria typical for phylogenetic group B2 deserved attention. Tests were carried out for the presence of 32 genes using the PCR method. Particularly noteworthy are the toxins Cnf-1, HlyA, Usp—an extensive iron uptake system (enterobactin, salmochelin, yersiniabactin and outer membrane hemin receptor ChuA)—SPATE autotransporters such as vat and pic, Ag43 autoaggregative protein—important for biofilm formation—and TosA/B which enhance the fitness of E.coli. All these virulence factors are identified predominantly in UPEC strains and provide a fitness advantage during colonization of the sinuses. Patients with CRS should be asked for past or present UTI. The specific virulence factors of E. coli that facilitate the colonization of the GI tract and urinary tract may also favor the colonization of a new ecological niche (sinuses) as a result of microbial imbalance or dysbiosis.
Feasibility of Known RNA Polymerase Inhibitors as Anti-SARS-CoV-2 Drugs
Authors: Neogi Ujjwal , Hill Kyle J. , Ambikan Anoop T , Heng Xiao , Quinn Thomas P. , Byrareddy Siddappa N. , Sönnerborg Anders , Sarafianos Stefan G. , Singh Kamal
Published: April 2020

Coronaviruses (CoVs) are positive-stranded RNA viruses that infect humans and animals. Infection by CoVs such as HCoV-229E, -NL63, -OC43 and -HKU1 leads to the common cold, short lasting rhinitis, cough, sore throat and fever. However, CoVs such as Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and the newest SARS-CoV-2 (the causative agent of COVID-19) lead to severe and deadly diseases with mortality rates ranging between ~1 to 35% depending on factors such as age and pre-existing conditions. Despite continuous global health threats to humans, there are no approved vaccines or drugs targeting human CoVs, and the recent outbreak of COVID-19 emphasizes an urgent need for therapeutic interventions. Using computational and bioinformatics tools, here we present the feasibility of reported broad-spectrum RNA polymerase inhibitors as anti- SARS-CoV-2 drugs targeting its main RNA polymerase, suggesting that investigational and approved nucleoside RNA polymerase inhibitors have potential as anti-SARS-CoV-2 drugs. However, we note that it is also possible for SARS-CoV-2 to evolve and acquire drug resistance mutations against these nucleoside inhibitors.
Emergence of Drift Variants That May Affect COVID-19 Vaccine Development and Antibody Treatment
Authors: Koyama Takahiko , Weeraratne Dilhan , Snowdon Jane L. , Parida Laxmi
Published: April 2020

New coronavirus (SARS-CoV-2) treatments and vaccines are under development to combat COVID-19. Several approaches are being used by scientists for investigation, including (1) various small molecule approaches targeting RNA polymerase, 3C-like protease, and RNA endonuclease; and (2) exploration of antibodies obtained from convalescent plasma from patients who have recovered from COVID-19. The coronavirus genome is highly prone to mutations that lead to genetic drift and escape from immune recognition; thus, it is imperative that sub-strains with different mutations are also accounted for during vaccine development. As the disease has grown to become a pandemic, B-cell and T-cell epitopes predicted from SARS coronavirus have been reported. Using the epitope information along with variants of the virus, we have found several variants which might cause drifts. Among such variants, 23403A>G variant (p.D614G) in spike protein B-cell epitope is observed frequently in European countries, such as the Netherlands, Switzerland, and France, but seldom observed in China.
Ecology of Neglected Rodent-Borne American Orthohantaviruses
Authors: Mull Nathaniel , Jackson Reilly , Sironen Tarja , Forbes Kristian M.
Published: April 2020

The number of documented American orthohantaviruses has increased significantly over recent decades, but most fundamental research has remained focused on just two of them: Andes virus (ANDV) and Sin Nombre virus (SNV). The majority of American orthohantaviruses are known to cause disease in humans, and most of these pathogenic strains were not described prior to human cases, indicating the importance of understanding all members of the virus clade. In this review, we summarize information on the ecology of under-studied rodent-borne American orthohantaviruses to form general conclusions and highlight important gaps in knowledge. Information regarding the presence and genetic diversity of many orthohantaviruses throughout the distributional range of their hosts is minimal and would significantly benefit from virus isolations to indicate a reservoir role. Additionally, few studies have investigated the mechanisms underlying transmission routes and factors affecting the environmental persistence of orthohantaviruses, limiting our understanding of factors driving prevalence fluctuations. As landscapes continue to change, host ranges and human exposure to orthohantaviruses likely will as well. Research on the ecology of neglected orthohantaviruses is necessary for understanding both current and future threats to human health.
Polyphenolic Profile and Varied Bioactivities of Processed Taiwanese Grown Broccoli: A Comparative Study of Edible and Non-Edible Parts
Authors: Le Thanh Ninh , Sakulsataporn Napat , Chiu Chiu-Hsia , Hsieh Pao-Chuan
Published: April 2020

Broccoli contains a substantial amount of bioactive compounds such as glucosinolates, phenolics, and essential nutrients, which are positively linked to health-promoting effects. This work aimed to evaluate whether both edible and non-edible parts of broccoli could be effective by examining in vitro antioxidant, cytotoxic, apoptotic, and antibacterial properties of its floret, leaf, and seed extracts (FE, LE, and SE, correspondingly). High-performance liquid chromatography (HPLC) and various assays exhibited strong antioxidant activities of all samples. LE obtained the highest capacity, correlated to its polyphenolic contents. SE exerted significant cytotoxicity against A549, Caco-2, and HepG2 cancer cell lines at low inhibitory concentration (IC)50 values (0.134, 0.209, and 0.238 mg/mL, respectively), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry confirmed apoptosis induction of extracts in Caco-2 cells by revealing an increased subG1 population and a decreased mitochondrial membrane potential. The considerable antibacterial efficacy was observed in either LE and SE against Bacillus subtilis and Salmonella typhimurium (0.39–0.78 mg/mL) using well-agar diffusion and minimum inhibitory concentration (MIC) techniques, along with the weak activity against Staphylococcus aureus and Escherichia coli (1.56–3.13 mg/mL). The findings suggest that broccoli and its byproducts might serve as a promising source for further development of food or pharmaceutical products.
Management of Descemet’s Membrane Detachment after Cataract Surgery—A Case Series
Authors: Huang Heng-Chiao , Jan Ren-Long , Tseng Sung-Huei , Lee Chia-Yi , Wei Fu-Tsung , Chang Yuh-Shin
Published: April 2020

Descemet’s membrane detachment (DMD) is an uncommon, vision-threatening, ocular surface complication of cataract surgery. Among several treatment strategies, sulfur hexafluoride (SF6) descemetopexy is the standard of care. Herein, we report three cases of DMD after cataract surgery managed with SF6 descemetopexy, showing different outcomes. Anatomical success was achieved in cases 1 and 2 while intraocular pressure (IOP) was elevated in case 2. In case 3, despite SF6 descemetopexy, recurrent DMD was observed. Due to persistent corneal edema and possible corneal decompensation in case 3, Descemet’s stripping automated endothelial keratoplasty was performed and a clear graft was found at the final visit. In conclusion, descemetopexy with 20 % SF6 is an effective and safe procedure for repairing DMD in most cases. Pupillary block with elevated IOP is another concern and prophylactic peripheral iridectomy is recommended. For recurrent DMDs, repeat descemetopexy could be considered. However, close monitoring is advocated since secondary management, such as endothelial keratoplasty, may be required.
Development of Digital Subterranean Models for Real-Time Open Cut Horizon Control
Authors: Strange Andrew D. , Jecny Zak
Published: April 2020

A reliable coal seam sensing system is required to improve the productivity of selective mining in open-cut mining operations. A prototype system based upon commercial ground penetrating radar equipment, which measures coal thickness from the top of an exposed surface down to an underlying coal-interburden interface and generates digital subterranean models of the subsurface seam boundaries, was developed for this purpose. The models can be deployed to commercially available in-cab assistive guidance systems for bulldozers and other mining machinery in existing production processes, and can further contribute to the databases required for remote operation and control in a complete digital mine scenario. The system was evaluated at a production open cut coal mine in Queensland, Australia, with promising results. The benefits reported by operational personnel who evaluated the digital surface model in the mining environment provide strong motivation for ongoing technology development.
Multispectral Models from Bare Soil Composites for Mapping Topsoil Properties over Europe
Authors: Safanelli José Lucas , Chabrillat Sabine , Ben-Dor Eyal , Demattê José A. M.
Published: April 2020

Reflectance of light across the visible, near-infrared and shortwave infrared (VIS-NIR-SWIR, 0.4–2.5 µm) spectral region is very useful for investigating mineralogical, physical and chemical properties of soils, which can reduce the need for traditional wet chemistry analyses. As many collections of multispectral satellite data are available for environmental studies, a large extent with medium resolution mapping could be benefited from the spectral measurements made from remote sensors. In this paper, we explored the use of bare soil composites generated from the large historical collections of Landsat images for mapping cropland topsoil attributes across the European extent. For this task, we used the Geospatial Soil Sensing System (GEOS3) for generating two bare soil composites of 30 m resolution (named synthetic soil images, SYSI), which were employed to represent the median topsoil reflectance of bare fields. The first (framed SYSI) was made with multitemporal images (2006–2012) framed to the survey time of the Land-Use/Land-Cover Area Frame Survey (LUCAS) soil dataset (2009), seeking to be more compatible to the soil condition upon the sampling campaign. The second (full SYSI) was generated from the full collection of Landsat images (1982–2018), which although displaced to the field survey, yields a higher proportion of bare areas for soil mapping. For evaluating the two SYSIs, we used the laboratory spectral data as a reference of topsoil reflectance to calculate the Spearman correlation coefficient. Furthermore, both SYSIs employed machine learning for calibrating prediction models of clay, sand, soil organic carbon (SOC), calcium carbonates (CaCO3), cation exchange capacity (CEC), and pH determined in water, using the gradient boosting regression algorithm. The original LUCAS laboratory spectra and a version of the data resampled to the Landsat multispectral bands were also used as reference of prediction performance using VIS-NIR-SWIR multispectral data. Our results suggest that generating a bare soil composite displaced to the survey time of soil observations did not improve the quality of topsoil reflectance, and consequently, the prediction performance of soil attributes. Despite the lower spectral resolution and the variability of soils in Europe, a SYSI calculated from the full collection of Landsat images can be employed for topsoil prediction of clay and CaCO3 contents with a moderate performance (testing R2, root mean square error (RMSE) and ratio of performance to interquartile range (RPIQ) of 0.44, 9.59, 1.77, and 0.36, 13.99, 1.54, respectively). Thus, this study shows that although there exist some constraints due to the spatial and temporal variation of soil exposures and among the Landsat sensors, it is possible to use bare soil composites for mapping key soil attributes of croplands across the European extent.
Geospatial Assessment of Water-Migration Scenarios in the Context of Sustainable Development Goals (SDGs) 6, 11, and 16
Authors: Nagabhatla Nidhi , Brahmbhatt Rupal
Published: April 2020

Communities and countries around the world are gearing up efforts to implement the 2030 Agenda goals and targets. In this paper, the water and migration scenarios are explained with a focus on Sustainable Development Goals (SDGs) 6 (water-related), 11 (urbanization), and 16 (peace and political stability). The study has two phases. The first phase illustrates the application of geospatial data and tools to assess the water-migration interlinkages (nexus) by employing a case study approach. Three case studies, Lake Chad, the Aral Sea region, and the Nile Delta, representing various geographic and socio-political settings, were selected to perform the multitemporal analysis. For this analysis, a mixed toolset framework that combined algorithmic functions of digital image processing, the Landsat sensor data, and applied a geographic information system (GIS) platform was adopted. How water-related events directly or indirectly trigger human migration is described using spatial indicators such as water spread and the extent of urban sprawl. Additionally, the geospatial outputs were analyzed in tandem with the climate variables such as temperature, precipitation data, and socio-economic variables such as population trends and migration patterns. Overall, the three case studies examined how water and climate crisis scenarios influence migration at a local and regional scale. The second phase showcases global-scale analysis based on the Global Conflict Risk Index (GCRI). This indicator reflects on the risks and conflicts with environmental, social, and political aspects and comments on the connection of these dimensions with migration. Together, the two phases of this paper provide an understanding ofthe interplay of water-related events on migration by applying the geospatial assessment and a proxy global index. Additionally, the paper reiterates that such an understanding can serve to establish facts and create evidence to inform sustainable development planning and decision making, particularly with regard to SDGs 6, 11, and 16. Targets such as 6.4 (managing water stress), 6.5 (transboundary challenges) and, 11.B (adaptation and resilience planning) can benefit from the knowledge generated by this geospatial exercise. For example, the high GCRI values for the African region speak to SDG targets 11.B (integrated policies/plans) and 16.7 (decision support systems for peaceful societies). Two key highlights from the synthesis: (a) migration and urbanization are closely interconnected, and (b) the impact of water and climate crisis is comparatively high for rural-urban migration due to the considerable dependence of rural communities on nature-based livelihoods. In conclusion, geospatial analysis is an important tool to study the interlinkages between water and migration. The paper presents a novel perspective toward widening the scope of remote sensing data and GIS toward the implementation of the SDG Agenda.
Surface Diffuse Solar Radiation Determined by Reanalysis and Satellite over East Asia: Evaluation and Comparison
Authors: Jiang Hou , Yang Yaping , Wang Hongzhi , Bai Yongqing , Bai Yan
Published: April 2020

Recently, surface diffuse solar radiation (Rdif) has been attracting a growing interest in view of its function in improving plant productivity, thus promoting global carbon uptake, and its impacts on solar energy utilization. To date, very few radiation products provide estimates of Rdif, and systematic validation and evaluation are even more scare. In this study, Rdif estimates from Reanalysis Fifth Generation (ERA5) of European Center for Medium-Range Weather Forecasts and satellite-based retrieval (called JiEA) are evaluated over East Asia using ground measurements at 39 stations from World Radiation Data Center (WRDC) and China Meteorological Administration (CMA). The results show that JiEA agrees well with measurements, while ERA5 underestimates Rdif significantly. Both datasets perform better at monthly mean scale than at daily mean and hourly scale. The mean bias error and root-mean-square error of daily mean estimates are −1.21 W/m2 and 20.06 W/m2 for JiEA and −17.18 W/m2 and 32.42 W/m2 for ERA5, respectively. Regardless of over- or underestimation, correlations of estimated time series of ERA5 and JiEA show high similarity. JiEA reveals a slight decreasing trend at regional scale, but ERA5 shows no significant trend, and neither of them reproduces temporal variability of ground measurements. Data accuracy of ERA5 is more robust than JiEA in time but less in space. Latitudinal dependency is noted for ERA5 while not for JiEA. In addition, spatial distributions of Rdif from ERA5 and JiEA show pronounced discrepancy. Neglect of adjacency effects caused by horizontal photon transport is the main cause for Rdif underestimation of ERA5. Spatial analysis calls for improvements to the representation of clouds, aerosols and water vapor for reproducing fine spatial distribution and seasonal variations of Rdif.
Sorting Objects from a Conveyor Belt Using POMDPs with Multiple-Object Observations and Information-Gain Rewards
Authors: Mezei Ady-Daniel , Tamás Levente , Buşoniu Lucian
Published: April 2020

We consider a robot that must sort objects transported by a conveyor belt into different classes. Multiple observations must be performed before taking a decision on the class of each object, because the imperfect sensing sometimes detects the incorrect object class. The objective is to sort the sequence of objects in a minimal number of observation and decision steps. We describe this task in the framework of partially observable Markov decision processes, and we propose a reward function that explicitly takes into account the information gain of the viewpoint selection actions applied. The DESPOT algorithm is applied to solve the problem, automatically obtaining a sequence of observation viewpoints and class decision actions. Observations are made either only for the object on the first position of the conveyor belt or for multiple adjacent positions at once. The performance of the single- and multiple-position variants is compared, and the impact of including the information gain is analyzed. Real-life experiments with a Baxter robot and an industrial conveyor belt are provided.
Overselling Globalization: The Misleading Conflation of Economic Globalization and Immigration, and the Subsequent Backlash
Authors: Castañeda Ernesto , Shemesh Amber
Published: April 2020

Many think that immigration is something caused by globalization, and some subsequently blame immigrants for the increased inequalities produced by economic globalization. Xenophobic nationalism has gained popularity around the world, further increasing racial tensions but without addressing the underlying causes of growing socioeconomic inequality, which this paper strives to show is caused by economic policies, not immigration. This paper argues that the apparent retreat from globalization arises from the flawed conceptualization of “globalization” as a bundle of different processes. This study analyzes early framings of economic globalization and shows how it has been linked, for political reasons, to increased migration, diversity, and open borders. Coining the term ”globalization” was not just naming ongoing social change, but it became part of the branding of an elite ideological policy project. The popular framing of globalization purposely entangled independent phenomena such as free trade policies, the expansion of the internet, cosmopolitan identities, and international migration. These couplings brought together neoliberal conservatives and liberal cosmopolitans. Given the current backlash, it is essential to distinguish migration from policies favoring trade and capital movement across borders. It is noteworthy to remember that immigration is something that preceded globalization. Therefore, it is necessary to investigate how migration became entangled with globalization in the popular imagination.
The Effects of Postprandial Resistance Exercise on Blood Glucose and Lipids in Prediabetic, Beta-Thalassemia Major Patients
Authors: Georgakouli Kalliopi , Stamperna Alexandra , Deli Chariklia K. , Syrou Niki , Draganidis Dimitrios , Fatouros Ioannis G. , Jamurtas Athanasios Z.
Published: April 2020

Insulin resistance and diabetes mellitus are common consequences of iron overload in the pancreas of beta-thalassemia major (BTM) patients. Moreover, postprandial blood glucose elevations are linked to major vascular complications. The purpose of this study was to investigate the effects of a bout of acute resistance exercise following breakfast consumption of glucose and fat on the metabolism in prediabetic, BTM patients. Six patients underwent two trials (exercise and control) following breakfast consumption (consisting of approximately 50% carbohydrates, 15% proteins, 35% fat), in a counterbalanced order, separated by at least three days. In an exercise trial, patients performed chest and leg presses (3 sets of 10 repetitions maximum/exercise), while in the control trial they rested. Blood samples were obtained in both trials at: pre-meal, 45 min post-meal (pre-exercise/control), post-exercise/control, 1 h post-exercise/control, 2 h post-exercise/control and 24 h post-exercise/control. Blood was analysed for glucose and lipids (total cholesterol, High Density Lipoprotein-cholesterol, Low Density Lipoprotein-cholesterol, triglycerides). Blood glucose levels increased significantly 45 min following breakfast consumption. Blood glucose and lipids did not differ between trials at the same time points. It seems that a single bout of resistance training is not sufficient to improve blood glucose and fat levels for the subsequent 24-h post-exercise period in prediabetic, BTM patients.