bluuebunny/crossref_metadata_2025 huggingface dataset:
Premise plumbing bacterial communities in four European cities and their association with Legionella
Authors: Scaturro Maria , Del Chierico Federica , Motro Yair , Chaldoupi Angeliki , Flountzi Anastasia , Moran-Gilad Jacob , Girolamo Antonietta , Koutsiomani Thomai , Krogulska Bozena , Lindsay Diane , Matuszewska Renata , Papageorgiou Georgios , Pancer Katarzyna , Panoussis Nikolaos , Rota Maria Cristina , Uldum Søren Anker , Velonakis Emmanuel , Chaput Dominique L. , Ricci Maria Luisa
Published: June 2023

Introduction

Legionella species are Gram negative, facultative, intracellular bacteria found in natural and engineered water systems. Understanding the bacterial interactions underlying the success of Legionella in aquatic environments could be beneficial for control.

Materials and methods

We aimed to profile, by 16S rRNA gene amplicon sequencing (V3-V4), the bacterial communities in premise plumbing systems of buildings in four European cities (Copenhagen, Warsaw, Rome, Athens), and identify positive and negative associations of specific community members to culturable Legionella.

Results

The coarse taxonomic composition was similar across the four cities, but Copenhagen and Warsaw had richer, more diverse communities than Athens and Rome, with a greater number of city-specific amplicon sequence variants (ASVs). The cities had statistically significant differences in bacterial communities at the ASV level, with relatively few shared ASVs. Out of 5,128 ASVs, 73 were classified as Legionella, and one or more of these were detected in most samples from each city (88.1% overall). Interestingly, the relative abundance of Legionella ASVs did not correlate with Legionella culture status. Overall, 44.2% of samples were Legionella culture positive: 71.4% in Warsaw, 62.2% in Athens, 22.2% in Rome, and 15.2% in Copenhagen. 54 specific ASVs and 42 genera had significant positive or negative associations with culturable Legionella. Negative associations included Staphylococcus, Pseudomonas, and Acinetobacter. Positive associations included several Nitrospira ASVs and one classified as Nitrosomodaceae oc32, ASVs in the amoeba-associated genera Craurococcus-Caldovatus and Reyranella, and the predatory genus Bdellovibrio.

Discussion

Some of these associations are well supported by laboratory studies, but others are the opposite of what was expected. This highlights the difficulties in translating pure culture results to in complex real-life scenarios. However, these positive and negative associations held across the four cities, across multiple buildings and plumbing compartments. This is important because developing better control measures, including probiotic approaches, will require an understanding of ecological relationships that can be generalised across different engineered water systems.
Bioethics and artificial intelligence: between deliberation on values and rational choice theory
Authors: Pinto-Bustamante Boris Julián , Riaño-Moreno Julián C. , Clavijo-Montoya Hernando Augusto , Cárdenas-Galindo María Alejandra , Campos-Figueredo Wilson David
Published: June 2023

The present work revisits how artificial intelligence, as technology and ideology, is based on the rational choice theory and the techno-liberal discourse, supported by large corporations and investment funds. Those that promote using different algorithmic processes (such as filter bubbles or echo chambers) create homogeneous and polarized spaces that reinforces people’s ethical, ideological, and political narratives. These mechanisms validate bubbles of choices as statements of fact and contravene the prerequisites for exercising deliberation in pluralistic societies, such as the distinction between data and values, the affirmation of reasonable dissent, and the relevance of diversity as a condition indispensable for democratic deliberation.
Case report: Portal vein ligation: lessons from patients with PRETEXT III hepatoblastoma in restoring future liver remnant before major hepatectomy and literature review
Authors: Gang Suiin , Ha Suhyeon , Kwon Hyunhee , Park Jueun , Namgoong Jung-Man
Published: June 2023

Background

We describe three cases involving three patients with PRETEXT III hepatoblastoma invading the hepatic hilum. After portal vein embolization, the patients underwent uncomplicated trisectionectomy.

Methods

Medical records between March 2016 and March 2021 were reviewed, and three patients were selected. A literature review of techniques for increasing future liver remnant in children diagnosed with hepatoblastoma was also conducted.

Results

All tumors involved the right lobe and hepatic hilum (PRETEXT III). After neoadjuvant chemotherapy, the tumor size decreased, but hilar involvement was unimproved. Right portal vein ligation (RPVL) was performed to increase the left lobe volume. Post-ligation, the remnant liver increased. Liver function was restored to normal levels within 5 days after the hepatectomy. All patients underwent two cycles of adjuvant chemotherapy without tumor recurrence.

Conclusions

RPVL can be safely performed before extended hepatic resection in children with a giant hepatoblastoma invading the hepatic hilum. The tumor was completely resected by securing a sufficient margin and increasing the residual liver volume with portal vein embolization. The patients recovered and underwent adjuvant chemotherapy without the deterioration of liver function.
The impact of the European Association of Endoscopic Surgery research grant scheme—a mixed qualitative quantitative methodology study protocol
Authors: McClean Adam , Huo Bright , Kwan Jing Yi , Long Judith , Walshaw Josephine , Mesri Mina , Francis Nader , Arulampalam Tan H. , Chetter Ian , Yiasemidou Marina
Published: June 2023

Background

The European Association of Endoscopic Surgery (EAES) is a surgical society who promotes the development and expansion of minimally invasive surgery to surgeons and surgical trainees. It does so through its activities in education, training, and research. The EAES research committee aims to promote the highest quality clinical research in endoscopic and minimally invasive surgery. They have provided grant funding since 2009 in education, surgery, and basic science. Despite the success and longevity of the scheme, the academic and non-academic impact of the research funding scheme has not been evaluated.

Aims

The primary aim of this project is to assess the short, long term academic and real world impact of the EAES funding scheme. The secondary aims are to identify barriers and facilitators for achieving good impact.

Methods

This will be a mixed qualitative and quantitative study. Semi-structured interviews will be performed with previous grant recipients. The questions for the interviews will be selected after a consensus is achieved amongst the members of the steering committee of this project. The responses will be transcribed and thematic analysis will be applied. The results of the thematic analysis will be used to populate a questionnaire which will be disseminated to grant recipients. This study is kindly funded by the EAES.

Discussion

The first question this project is expected to answer is whether the EAES research funding scheme had a significant positive impact on research output, career progression but also non-academic output such as change in clinical guidelines, healthcare quality and cost-effectiveness improvement. This project however is also expected to identify facilitators and barriers to successful completion of projects and to achieving high impact. This will inform EAES and the rest of the surgical and academic communities as to how clinicians would like to be supported when conducting research. There should also be a positive and decisive change towards removing factors that hinder the timely and successful completion of projects.
Alternatives to antibiotics for treatment of mastitis in dairy cows
Authors: Li Xiaoping , Xu Chuang , Liang Bingchun , Kastelic John P. , Han Bo , Tong Xiaofang , Gao Jian
Published: June 2023

Mastitis is considered the costliest disease on dairy farms and also adversely affects animal welfare. As treatment (and to a lesser extent prevention) of mastitis rely heavily on antibiotics, there are increasing concerns in veterinary and human medicine regarding development of antimicrobial resistance. Furthermore, with genes conferring resistance being capable of transfer to heterologous strains, reducing resistance in strains of animal origin should have positive impacts on humans. This article briefly reviews potential roles of non-steroidal anti-inflammatory drugs (NSAIDs), herbal medicines, antimicrobial peptides (AMPs), bacteriophages and their lytic enzymes, vaccination and other emerging therapies for prevention and treatment of mastitis in dairy cows. Although many of these approaches currently lack proven therapeutic efficacy, at least some may gradually replace antibiotics, especially as drug-resistant bacteria are proliferating globally.
The effect of acute equine temporomandibular joint inflammation on response to rein-tension and kinematics
Authors: Reisbig Nathalie A. , Pifko Justin , Lanovaz Joel L. , Weishaupt Michael A. , Carmalt James L.
Published: June 2023

Background

Although the temporomandibular joint (TMJ) is the major contact point between the reins in the riders’ hand, the bit in the mouth, and the rest of the horse under saddle, the role of inflammation of this joint on equine locomotion and rein tension is unknown.

Objective

To determine the effect of acute TMJ inflammation on rein-tension and horse movement when horses were long-reined on a treadmill.

Study design

A randomized, controlled, cross-over design.

Methods

Five horses were trained by one clinician to walk and trot on a treadmill wearing long-reining equipment instrumented with a rein-tension device and reflective optical tracking markers. Subjective assessment of horse’s dominant side, and movement, were determined without rein-tension (free walk and trot); and with rein-tension (long-reined walk and trot). Continuous rein-force data from both sides were collected over ~60s from each trial. Movement was recorded using a 12-camera optical motion capture system. One randomly assigned TMJ was subsequently injected with lipopolysaccharide and the treadmill tests repeated by investigators blinded to treatment side. A second, identical assessment was performed 10 days later with the opposite TMJ being the target of intervention.

Results

All horses showed reduced rein-tension on the injected (inflamed) side. Increased rein-tension was required on the non-injected side at trot, to maintain them in the correct position on the treadmill post-injection. The only kinematic variable to show any significant change due to rein tension or TMJ inflammation during the walk or trot was an increase in forward head tilt in the presence of rein tension in the trot after injection.

Main limitations

Low number of horses and investigation of response to acute inflammation only.

Conclusion

TMJ inflammation changed, subjectively and objectively, the response to rein-input, but the horses did not become lame.
Fault-Diagnosis Method for Rotating Machinery Based on SVMD Entropy and Machine Learning
Authors: Zhang Lijun , Zhang Yuejian , Li Guangfeng
Published: June 2023

Rolling bearings and gears are important components of rotating machinery. Their operating condition affects the operation of the equipment. Fault in the accessory directly leads to equipment downtime or a series of adverse reactions in the system, which brings enormous pecuniary loss to the institution. Hence, it is of great significance to detect the operating status of rolling bearings and gears for fault diagnosis. At present, the vibration method is considered to be the most common method for fault diagnosis, a method that analyzes the equipment by collecting vibration signals. However, rotating-machinery fault diagnosis is challenging due to the need to select effective fault feature vectors, use appropriate machine-learning classification methods, and achieve accurate fault diagnosis. To solve this problem, this paper illustrates a new fault-diagnosis method combining successive variational-mode decomposition (SVMD) entropy values and machine learning. First, the simulation signal and the real fault signal are used to analyze and compare the variational-mode decomposition (VMD) and SVMD methods. The comparison results prove that SVMD can be a useful method for fault diagnosis. Then, these two methods are utilized to extract the energy entropy and fuzzy entropy of the gearbox dataset of Southeast University (SEU), respectively. The feature vector and multiple machine-learning classification models are constructed for failure-mode identification. The experimental-analysis results successfully verify the effectiveness of the combined SVMD entropy and machine-learning approach for rotating-machinery fault diagnosis.
Combination of Graphene Oxide and Rhizobium Improved Soybean Tolerance in Saline-Alkali Stress
Authors: Fu Xiaohong , Bian Dahong , Gu Xuyang , Cao Jinfeng , Liu Jianfeng
Published: June 2023

Soybean (Glycine max L.) is the most important crop plant in the world. Soil saline-alkali seriously inhibits soybean yield and quality. This study aims to investigate the impact of graphene oxide (GO) and Rhizobium (Rh) on the expression of soybean-related genes as well as the growth and yield under saline-alkali stress. The results show that GO + Rh-treated increased the number of root nodules by 5.43 times compared with the control (Ctrl), the total nitrogen content and root system parameters of plants were also significantly improved. GO + Rh-treated reduced the Na+/K+ ratio and the osmotic substances, while the activities of antioxidant enzymes SOD, POD, CAT and APX in GO + Rh-treated soybean plants increased significantly by 69.18%, 69%, 75.64% and 48.38% compared with the control plants. The REC, MDA and H2O2 content decreased significantly by 46.73%, 42.80% and 43.53%. In addition, GA3 content, among all related saline-alkali hormones, was increased by 100.20% compared with the Ctrl. The expression level of GmGBP1, a key gene for GA3 synthesis, at most increased 6.42 times compared to the Ctrl. The results further reveal that GO + Rh-treated obviously improves the yield traits of soybean plants, which confirms that GO + Rh-treated could be effective in enhancing soybean tolerance to saline-alkali stress. Our findings provide a new strategy for improving the saline-alkali tolerance of soybean, as well as a new perspective for exploiting and utilizing large-area saline-alkali soil.
The Effect of Foliar Zinc Application on the Leaf Chlorophyll Concentrations and Grain Yields of the Winter Wheat (Triticum aestivum L.) in the Field Experiments of Two Seasons
Authors: Irmes Katalin , Kristó István , Szentpéteri Lajos , Rácz Attila , Vályi-Nagy Marianna , Kassai Mária Katalin , Valentinyi Klára Veresné , Tar Melinda
Published: June 2023

This research examined the effectiveness of zinc foliar fertilizers. Two winter wheat varieties, GK Pilis and Zvezdana, were tested in a Zn-limited meadow chernozem soil. Crop yields and chlorophyll concentrations of the leaves were examined in the growing seasons of 2020/2021 of favorable weather conditions and 2021/2022 of drought weather conditions. In the experiment, three treatments were carried out with monozinc foliar fertilizer of a concentrated 12% (w/v) zinc sulfate heptahydrate solution, and the fourth plot was designed as the untreated control. The first monozinc treatment was applied at a dose of 1 L ha−1 and the second monozinc treatment at a dose of 2 L ha−1. These treatments were applied at tillering (BBCH 21–22). The third monozinc treatment was applied at a dose of 1 L ha−1 at tillering and flag leaf emergence (BBCH 21–22, BBCH 37). Samples were taken several times in the vegetation period, and measurements were made using different methods (SPAD, determination of total chlorophyll concentration from acetone solution) before and after Zn treatments. In 2020/2021 of favorable weather conditions, the yield of Zvezdana was 5.47 t ha−1 and that of Gk Pilis was 6.11 t ha−1, compared to 2021/2022 of drought weather conditions, in which year the yield of Zvezdana was 3.14 t ha−1 and that of Gk Pilis was 2.79 t ha−1. Yields of both varieties increased with increasing NPK nutrient doses. In 2021/2022, even at nutrient level D (NPK basal fertilizer and two-times N topdressing), we harvested on average twice as much as in 2020/2021 at nutrient level A (N basal fertilizer and once N topdressing). No significant effects were found between the foliar fertilizers in 2020/2021. In 2021/2022, Zn treatments, mainly at four nutrient levels tended to increase the yields of both varieties. The first treatment resulted in a grain yield reduction of 3% for GK Pilis, and the second treatment resulted in a grain yield reduction of 1.2% for Zvezdana. After the second and third treatments, grain yield reductions were 0.1%–0.5%. Although there was no significant difference in terms of the main averages, foliar fertilizer was found to be effective in the second year. The relationship between chlorophyll and grain yield was found to depend on the year and variety. A weak correlation (r = 0.33, p < 0.05) was found between the total chlorophyll concentration in leaves for Zvezdana in 2021, and a medium correlation for GK Pilis (r = 0.67, p < 0.05). Concerning grain yield and chlorophyll concentration, a strong correlation (r = 0.81, p < 0.05) was found for Zvezdana in 2022, and a weak correlation (r = 0.44, p < 0.05) for GK Pilis. A strong correlation was found between the total leaf chlorophyll concentration and SPAD index in both years (r = 0.81, p < 0.05)
Carry-Over Effects of Climate Variability at Breeding and Non-Breeding Grounds on Spring Migration in the European Wren Troglodytes troglodytes at the Baltic Coast
Authors: Gołębiewski Ignacy , Remisiewicz Magdalena
Published: June 2023

Many studies have linked changes in avian phenology in Europe to the North Atlantic Oscillation (NAO), which serves as a proxy for conditions in western Europe. However, the effects of climate variation in other regions of Europe on the phenology of short-distance migrants with large non-breeding grounds remain unclear. We determined the combined influence of large-scale climate indices, NAO, the Mediterranean Oscillation Index (MOI), and the Scandinavian Pattern (SCAND), during the preceding year on spring migration timing of European wren at the southern Baltic coast during 1982–2021. We modelled the effects of these climate variables on the entire passage and subsequent percentiles of the wren’s passage at Bukowo-Kopań and Hel ringing stations. Over 1982–2021, the start and median of migration shifted earlier at Hel, but the end of passage shifted later at both stations. In effect, the duration of passage at Hel was extended by 7.6 days. Early passage at Hel was related with high MOI in spring and the preceding autumn. Spring passage at Bukowo-Kopań was delayed after high NAO in the previous breeding season, and high winter and spring NAO. Late spring passage occurred at both stations following a high SCAND in the previous summer. At both locations, an early start or median of passage followed high local temperatures. We conclude that phenology of the wren’s spring migration at the Baltic coast was shaped by conditions encountered at wintering quarters in western Europe, where NAO operates, and in the south-eastern Europe, where the MOI operates, in conjunction with conditions in Scandinavia during the previous breeding season. We demonstrated that climate variability in various parts of the migrants’ range has combined carry-over effects on in migrants’ phenology in Europe.
Insight into the Clonal Lineage and Antimicrobial Resistance of Staphylococcus aureus from Vascular Access Infections before and during the COVID-19 Pandemic
Authors: Kao Chih-Chen , Lai Chi-Hsiang , Wong Min-Yi , Huang Tsung-Yu , Tseng Yuan-Hsi , Lu Chu-Hsueh , Lin Chien-Chao , Huang Yao-Kuang
Published: June 2023

Patients receiving hemodialysis are at risk of vascular access infections (VAIs) and are particularly vulnerable to the opportunistic pathogen Staphylococcus aureus. Hemodialysis patients were also at increased risk of infection during the COVID-19 pandemic. Therefore, this study determined the change in the molecular and antibiotic resistance profiles of S. aureus isolates from VAIs during the pandemic compared with before. A total of 102 S. aureus isolates were collected from VAIs between November 2013 and December 2021. Before the pandemic, 69 isolates were collected, 58%, 39.1%, and 2.9% from arteriovenous grafts (AVGs), tunneled cuffed catheters (TCCs), and arteriovenous fistulas (AVFs), respectively. The prevalence of AVG and TCC isolates changed to 39.4% and 60.6%, respectively, of the 33 isolates during the pandemic. Sequence type (ST)59 was the predominant clone in TCC methicillin-resistant S. aureus (MRSA) and AVG-MRSA before the pandemic, whereas the predominant clone was ST8 in AVG-MRSA during the pandemic. ST59 carrying the ermB gene was resistant to clindamycin and erythromycin. By contrast, ST8 carrying the msrA gene was exclusively resistant to erythromycin. The ST distribution for different VAIs changed from before to during the pandemic. The change in antibiotic resistance rate for different VAIs was closely related to the distribution of specific STs.
Delaying the Occurrence of Bar Buckling in RC Columns Confined with SRG Jacketing
Authors: Thermou Georgia E. , Tastani Sousana
Published: June 2023

This paper investigates experimentally the structural performance of substandard reinforced concrete (RC) short columns confined with steel-reinforced grout (SRG) jackets under monotonically increasing uniaxial compression. The study comprised 24 square cross section short RC columns having alternative arrangements of shear reinforcement (ratio of stirrup spacing to longitudinal bar diameter ranging from 4.2 to 12.5). The short columns were retrofitted with externally applied SRG jacketing differing by the density of the fabric (4 cords/in and 12 cords/in) and the number of fabric layers (1 and 2). The test results showed that retrofitting significantly changed the behaviour of the specimens compared to the unconfined counterparts. For columns at risk of premature failure due to insufficient support of compression bars provided by the sparse stirrups, the SRG jackets delayed bar buckling, enabling the members to achieve greater strength and deformation capacity. The well-detailed specimens helped establish the maximum effectiveness of SRG confinement.
Energy and Environmental Performance Comparison of Heat Pump Systems Working with Alternative Refrigerants
Authors: Dongellini Matteo , Natale Christian , Naldi Claudia , Rossi di Schio Eugenia , Valdiserri Paolo , Morini Gian Luca
Published: June 2023

The European Parliament has imposed to reduce by 2030 whole HFC emissions by at least two-thirds with respect to 2014 levels. With the aim of contributing to determine the energy and environmental advantages of refrigerants alternative to R-410A, this paper reports the results of a numerical study focused on an HVAC system coupled to a residential building and based on a reversible electric heat pump. In particular, two heat pump typologies are considered: an air-source and a ground-source heat pump, both operating with the two refrigerants R-410A and R-454B. The environmental performance of the studied system is assessed by means of the TEWI (total equivalent warming impact) index. The adoption of R-454B involves a slight decrease (2–3%) in the overall annual energy performance of the system with respect to the use of R-410A. On the other hand, the working fluid R-454B guarantees a marked decrease in the TEWI indicator. Indeed, considering the current Italian emission factor of electricity taken from the grid, the total emissions over the entire heat pump operating life drop by about 25% and can decrease by up to 89% in perspective, following the current reduction trend of the emission factor.
Research Control Devices for LED Light Sources under Their Operating Conditions at Elevated Temperatures
Authors: Beliakova Iryna , Piscio Vadim , Maruschak Pavlo , Shovkun Oleksandr , Medvid Volodymyr , Mykhailyshyn Roman
Published: June 2023

The electrical and light characteristics of light-emitting diodes (LEDs), upon which modern lighting devices are constructed, are significantly influenced by ambient temperature. As the temperature rises, the luminous flux of LEDs diminishes, and the lifespan of LED devices also decreases. The control devices (drivers) used in LED lighting systems, built based on current stabilization schemes, do not effectively stabilize the luminous flux when the ambient temperature fluctuates between +15 to +60 °C. The luminous flux declines as the temperature increases, even if the current supplied to the LEDs remains constant. This paper presents a comparative analysis of LED lighting devices equipped with drivers featuring current stabilization and those with drivers employing voltage stabilization for operation in higher temperature conditions.
Heat and Moisture Induced Stress and Strain in Wooden Artefacts and Elements in Heritage Buildings: A Review
Authors: Grottesi Giulia , Coelho Guilherme B. A. , Kraniotis Dimitrios
Published: June 2023

In the world of cultural heritage, a wide range of artefacts and buildings are made of wood and, therefore, are subjected to moisture-induced stress and strain cycles, owing to environmental fluctuations. Simultaneous action of moisture and mechanical loads lead to a mechanosorptive effect on wood. Therefore, an increase in time-dependent creep, due to mechanical loads, is observed. The assessment of these complex phenomena entails the use of advance and interdisciplinary approaches. Consequently, this article reviews experimental and mathematical methods to study these degradation mechanisms in wooden artefacts and timber elements in heritage buildings. The paper presents the results of a six-step descriptive literature review, providing an overall picture of the ongoing research. Experimental techniques need to be improved so that they are in line with the conservation principles. The combination of experiments and simulations is a reliable predictive approach for better assessing the potential risk damages due to temperature, humidity cycles, and mechanical loads in complex structures. Thus, advanced numerical simulations and mathematical modelling include climate data and experimental measurements. This work also provides an overview of research performed on different categories of cultural heritage characterised by multi-layer structures. The mechanical response to wood–moisture relation is affected by the level of complexity of these structures. Finally, the use of realistic models is limited by knowledge about the material properties and the behaviour of complex structures over time. In addition, research gaps, limitations, and possible future research directions are also provided. This review may represent a starting point for future research on the thermo-hygro-mechanical behaviour of wood heritage.
A Machine Learning Method with Hybrid Feature Selection for Improved Credit Card Fraud Detection
Authors: Mienye Ibomoiye Domor , Sun Yanxia
Published: June 2023

With the rapid developments in electronic commerce and digital payment technologies, credit card transactions have increased significantly. Machine learning (ML) has been vital in analyzing customer data to detect and prevent fraud. However, the presence of redundant and irrelevant features in most real-world credit card data degrades the performance of ML classifiers. This study proposes a hybrid feature-selection technique consisting of filter and wrapper feature-selection steps to ensure that only the most relevant features are used for machine learning. The proposed method uses the information gain (IG) technique to rank the features, and the top-ranked features are fed to a genetic algorithm (GA) wrapper, which uses the extreme learning machine (ELM) as the learning algorithm. Meanwhile, the proposed GA wrapper is optimized for imbalanced classification using the geometric mean (G-mean) as the fitness function instead of the conventional accuracy metric. The proposed approach achieved a sensitivity and specificity of 0.997 and 0.994, respectively, outperforming other baseline techniques and methods in the recent literature.
Predicting Critical Nodes in Temporal Networks by Dynamic Graph Convolutional Networks
Authors: Yu Enyu , Fu Yan , Zhou Junlin , Sun Hongliang , Chen Duanbing
Published: June 2023

Many real-world systems can be expressed in temporal networks with nodes playing different roles in structure and function, and edges representing the relationships between nodes. Identifying critical nodes can help us control the spread of public opinions or epidemics, predict leading figures in academia, conduct advertisements for various commodities and so on. However, it is rather difficult to identify critical nodes, because the network structure changes over time in temporal networks. In this paper, considering the sequence topological information of temporal networks, a novel and effective learning framework based on the combination of special graph convolutional and long short-term memory network (LSTM) is proposed to identify nodes with the best spreading ability. The special graph convolutional network can embed nodes in each sequential weighted snapshot and LSTM is used to predict the future importance of timing-embedded features. The effectiveness of the approach is evaluated by a weighted Susceptible-Infected-Recovered model. Experimental results on four real-world temporal networks demonstrate that the proposed method outperforms both traditional and deep learning benchmark methods in terms of the Kendall τ coefficient and top k hit rate.
Analysis of Carbon Particulate Matter Removal Performance of Dual-Fuel Marine Engine with DOC + CDPF
Authors: Yang Zhiyuan , Tan Zhiwen , Tan Qinming , Tu Shien
Published: June 2023

This study analyzes Diesel Oxidation Catalyst (DOC) and Carbon Diesel Particulate Filter (CDPF) after-treatment systems integrated into a WARTSILA W20DF marine dual-fuel engine. The CDPF was coated with a non-precious metal catalyst whose catalytic redox performance improved with increasing temperature. The carbon particulate matter combustion reached up to 12.5 mg/s at 800 K and over 20 mg/s at 900 K. Then, the W20DF running at 230 kW, 450 kW, 680 kW, and 810 kW with 1000 rpm; a Tisch 10-8xx; and an AVL SPC 478 were used to sample and analyze the carbon particulate matter (CPM) before and after DOC + CDPF. The gaseous emissions (O2, CO2, CO, HC, NOx, and NO2) were analyzed with the flue gas analyzer AVL i60. The results show that the collected carbon particulate matter simultaneously became darker as the load decreased. This study finds that the maximum amount of CPM per unit volume of exhaust gas occurs under 50% working conditions and the lowest amount under 90% working conditions. After DOC + CDPF treatment with a non-precious metal coating, the CPM was reduced by about 50%. Furthermore, this type of catalyst’s efficiency rises with the temperature increase. The CPM combustion efficiency reached up to 20 mg/s at 900 K. The other gas components in the exhaust gas before and after DOC + CDPF also changed. These research results have a significant reference value for DOC + CDPF optimization to decrease the carbon particulate matter in marine engines.
Underrepresentation of the Linkage between the Barents–Kara Sea Ice and East Asian Rainfall in Early Summer by CMIP6 Models
Authors: Chen Haohan , Rao Jian , Yang Huidi , Luo Jingjia , Wu Gangsen
Published: June 2023

Our previous study revealed the link between Barents–Kara sea ice and rainfall in eastern China. This study continues evaluating the performance of multiple models from phase 6 of the Coupled Model Intercomparison Project (CMIP6) in simulating this linkage. Most CMIP6 models can simulate Arctic sea ice coverage in the present climate system, although the sea ice extent in the edge areas show some biases. Only a few models can roughly reproduce the observed rainfall dipole pattern associated with Arctic sea ice variability. The linkage between Arctic sea ice variability in winter and eastern China rainfall in early summer is performed through a long memory of the sea ice, the stratospheric variability as the mediator, and downward propagation of stratospheric signals. Very few CMIP6 models can exhibit a realistic interannual relationship between the Arctic sea ice and China rainfall. The selected high-skill models with a more realistic linkage between sea ice and China rainfall present a clear downward impact of the stratospheric circulation anomalies associated with sea ice variability. The reversal of the Northern Hemisphere Annular Mode (NAM) from the negative phase in early winter to the positive phase in spring in the high-skill models and observations denotes the important role of the stratosphere as a mediator to bridge the Arctic sea ice and China rainfall. The long memory of the Arctic sea ice with the stratosphere as the mediator has a deep implication on the seasonal forecasts of East Asian countries.
A Nonconstant Gradient Constrained Problem for Nonlinear Monotone Operators
Authors: Giuffrè Sofia
Published: June 2023

The purpose of the research is the study of a nonconstant gradient constrained problem for nonlinear monotone operators. In particular, we study a stationary variational inequality, defined by a strongly monotone operator, in a convex set of gradient-type constraints. We investigate the relationship between the nonconstant gradient constrained problem and a suitable double obstacle problem, where the obstacles are the viscosity solutions to a Hamilton–Jacobi equation, and we show the equivalence between the two variational problems. To obtain the equivalence, we prove that a suitable constraint qualification condition, Assumption S, is fulfilled at the solution of the double obstacle problem. It allows us to apply a strong duality theory, holding under Assumption S. Then, we also provide the proof of existence of Lagrange multipliers. The elements in question can be not only functions in L2, but also measures.
Association between Low-Density Lipoprotein Cholesterol and Vascular Biomarkers in Primary Prevention
Authors: Kozakova Michaela , Morizzo Carmela , Jamagidze Giuli , Della Latta Daniele , Chiappino Sara , Chiappino Dante , Palombo Carlo
Published: June 2023

Several noninvasive vascular biomarkers have been proposed to improve risk stratification for atherothrombotic events. To identify biomarkers suitable for detecting intermediate-risk individuals who might benefit from lipid-lowering treatment in primary prevention, the present study tested the association of plasma LDL-cholesterol with coronary artery calcification (CAC) Agatston score, high carotid and femoral intima-media thickness (IMT), low carotid distensibility and high carotid-femoral pulse-wave velocity in 260 asymptomatic individuals at intermediate cardiovascular risk and without diabetes and lipid-lowering treatment. High or low vascular biomarkers were considered when their value was above the 95th or below the 5th percentile, respectively, of the distribution in the healthy or in the study population. LDL-cholesterol was independently associated with the CAC score = 0 (OR 0.67; 95%CI 0.48–0.92, p = 0.01), CAC score > 100 (1.59; 1.08–2.39, p = 0.01) and high common femoral artery (CFA) IMT (1.89; 1.19–3.06, p < 0.01), but not with other biomarkers. Our data confirm that in individuals at intermediate risk, lipid-lowering treatment can be avoided in the presence of a CAC score = 0, while it should be used with a CAC score > 100. CFA IMT could represent a useful biomarker for decisions regarding lipid-lowering treatment. However, sex- and age-specific reference values should be established in a large healthy population.
Numerosity Comparison in Three Dimensions in the Case of Low Numerical Values
Authors: Aida Saori
Published: June 2023

This study investigated the perception of numbers in humans in 3D stimuli. Recent research has shown that number processing relies on “number sense” for small values, in line with Weber’s law. While previous studies have reported 3D numerosity overestimation mainly in higher numerical values, our experiment examined whether this phenomenon occurs at lower numerical values. We also explored whether the Weber ratio follows Weber’s law when comparing 2D and 3D stimuli in terms of the number of elements. Observers were presented with pairs of stimuli on a monitor and were asked to identify the stimulus with a larger number of elements. Using the constant method, we calculated the point of subjective equality (PSE), just noticeable difference (JND), and Weber ratios from the collected data. As a result, it was confirmed that the phenomenon of over-estimation of 3D numerical values occurs even when the numerical values are small. Additionally, we observed that the Weber fraction adhered to Weber’s law within the measured range. These findings contribute to the existing body of research, supporting the existence of distinct mechanisms for perceiving numerosity and density.
Tools for the Assessment of Risk-Taking Behavior in Older Adults with Mild Dementia: A Cross-Sectional Clinical Study
Authors: Compagne Charline , Gabriel Damien , Ferrero Lénaïc , Magnin Eloi , Tannou Thomas
Published: June 2023

Diseases such as Alzheimer’s cause an alteration of cognitive functions, which can lead to increased daily risk-taking in older adults living at home. The assessment of decision-making abilities is primarily based on clinicians’ global analysis. Usual neuropsychological tests such as the MoCA (Montreal Cognitive Assessment) cover most of the cognitive domains and include mental flexibility tasks. Specific behavioral tasks for risk-taking, such as the Balloon Analogue Risk Task (BART) or the Iowa Gambling Task (IGT), have been developed to assess risk-taking behavior, particularly in the field of addictology. Our cross-sectional study aims to determine whether the MoCA global cognitive assessment could be used as a substitute for behavioral tasks in the assessment of risky behavior. In the current study, 24 patients (age: 82.1 ± 5.9) diagnosed with mild dementia completed the cognitive assessment (MoCA and executive function assessment) and two behavioral risk-taking tasks (BART, simplified version of the IGT). Results revealed no relationship between scores obtained in the MoCA and behavioral decision-making tasks. However, the two tasks assessing risk-taking behavior resulted in concordant risk profiles. In addition, patients with a high risk-taking behavior profile on the BART had better Trail Making Test (TMT) scores and thus retained mental flexibility. These findings suggest that MoCA scores are not representative of risk-taking behavioral inclinations. Thus, additional clinical tests should be used to assess risk-taking behavior in geriatric settings. Executive function measures, such as the TMT, and behavioral laboratory measures, such as the BART, are recommended for this purpose.
The RHOA Mutation G17V Does Not Lead to Increased Migration of Human Malignant T Cells but Is Associated with Matrix Remodelling
Authors: Merk-Ahmad Katrin , Bein Julia , Scharf Sonja , Schäfer Hendrik , Bexte Tobias , Ullrich Evelyn , Loth Andreas G. , Flinner Nadine , Senff Tina , Schneider Olga , Hansmann Martin-Leo , Piel Matthieu , Häupl Björn , Oellerich Thomas , Donnadieu Emmanuel , Hartmann Sylvia
Published: June 2023

Nodal T-follicular helper cell lymphoma, angioimmunoblastic-type (AITL), is characterized by constitutional symptoms, advanced-stage disease, and generalized lymphadenopathy. A genetic hallmark of this lymphoma is the frequent occurrence of the RHOA mutation G17V in neoplastic cells, which is observed in around 60% of patients. Because RHOA is involved in both T-cell receptor downstream signalling and cell migration, we hypothesized that the characteristic presentation of AITL could be the result of enhanced tumor cell migration. Therefore, this study aimed to elucidate the impact of the RHOA variant G17V on the migration of neoplastic T cells. We transfected the T-cell lymphoma cell lines HH and HuT78 to stably express the RHOA-G17V variant. RHOA-G17V-expressing T cells did not exhibit enhanced motility compared to empty-vector-transfected cells in microchannels, a 3D collagen gel, or primary human lymphatic tissue. Cells of the HH cell line expressing RHOA-G17V had an increased number of cells with cleaved collagen compared with the empty-vector-transfected cells. Therefore, we hypothesized that the early spread of AITL tumor cells may be related to remodelling of the extracellular matrix. Accordingly, we observed a significant negative correlation between the relative area of collagen in histological sections from 18 primary AITL and the allele frequency of the RHOA-G17V mutation. In conclusion, our results suggest that the characteristic presentation of AITL with early, widespread dissemination of lymphoma cells is not the result of an enhanced migration capacity due to the RHOA-G17V mutation; instead, this feature may rather be related to extracellular matrix remodelling.
Drug-Eluting Embolic Loaded with Tyrosine Kinase Inhibitor Targeted Therapies for Transarterial Chemoembolization in a VX2 Model
Authors: Abi-Jaoudeh Nadine , Sadeghi Ben , Javan Hanna , Na Jim , Beaton Graham , Tucci Fabio , Ravula Satheesh , Imagawa David K.
Published: June 2023

Drug-eluting embolic transarterial chemoembolization (DEE-TACE) improves the overall survival of hepatocellular carcinoma (HCC), but the agents used are not tailored to HCC. Our patented liposomal formulation enables the loading and elution of targeted therapies onto DEEs. This study aimed to establish the safety, feasibility, and pharmacokinetics of sorafenib or regorafenib DEE-TACE in a VX2 model. DEE-TACE was performed in VX2 hepatic tumors in a selective manner until stasis using liposomal sorafenib- or regorafenib-loaded DEEs. The animals were euthanized at 1, 24, and 72 h timepoints post embolization. Blood samples were taken for pharmacokinetics at 5 and 20 min and at 1, 24, and 72 h. Measurements of sorafenib or regorafenib were performed in all tissue samples on explanted hepatic tissue using the same mass spectrometry method. Histopathological examinations were carried out on tumor tissues and non-embolized hepatic specimens. DEE-TACE was performed on 23 rabbits. The plasma concentrations of sorafenib and regorafenib were statistically significantly several folds lower than the embolized liver at all examined timepoints. This study demonstrates the feasibility of loading sorafenib or regorafenib onto commercially available DEEs for use in TACE. The drugs eluted locally without release into systemic circulation.
Peptide Lv Promotes Trafficking and Membrane Insertion of KCa3.1 through the MEK1–ERK and PI3K–Akt Signaling Pathways
Authors: Pham Dylan L. , Niemi Autumn , Blank Ria , Lomenzo Gabriella , Tham Jenivi , Ko Michael L. , Ko Gladys Y.-P.
Published: June 2023

Peptide Lv is a small endogenous secretory peptide that is proangiogenic through hyperpolarizing vascular endothelial cells (ECs) by enhancing the current densities of KCa3.1 channels. However, it is unclear how peptide Lv enhances these currents. One way to enhance the current densities of ion channels is to promote its trafficking and insertion into the plasma membrane. We hypothesized that peptide Lv-elicited KCa3.1 augmentation occurs through activating the mitogen-activated protein kinase kinase 1 (MEK1)-extracellular signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K)–protein kinase B (Akt) signaling pathways, which are known to mediate ion channel trafficking and membrane insertion in neurons. To test this hypothesis, we employed patch-clamp electrophysiological recordings and cell-surface biotinylation assays on ECs treated with peptide Lv and pharmaceutical inhibitors of ERK and Akt. Blocking ERK or Akt activation diminished peptide Lv-elicited EC hyperpolarization and increase in KCa3.1 current densities. Blocking PI3K or Akt activation decreased the level of plasma membrane-bound, but not the total amount of KCa3.1 protein in ECs. Therefore, the peptide Lv-elicited EC hyperpolarization and KCa3.1 augmentation occurred in part through channel trafficking and insertion mediated by MEK1–ERK and PI3K–Akt activation. These results demonstrate the molecular mechanisms of how peptide Lv promotes EC-mediated angiogenesis.
Human Adult Renal Progenitor Cells Prevent Cisplatin-Nephrotoxicity by Inducing CYP1B1 Overexpression and miR-27b-3p Down-Regulation through Extracellular Vesicles
Authors: Franzin Rossana , Stasi Alessandra , De Palma Giuseppe , Picerno Angela , Curci Claudia , Sebastiano Serena , Campioni Monica , Cicirelli Antonella , Rizzo Alessandro , Di Lorenzo Vito Francesco , Pontrelli Paola , Pertosa Giovanni Battista , Castellano Giuseppe , Gesualdo Loreto , Sallustio Fabio
Published: June 2023

Cisplatin is one of the most effective chemotherapeutic agents strongly associated with nephrotoxicity. Tubular adult renal progenitor cells (tARPC) can regenerate functional tubules and participate in the repair processes after cisplatin exposition. This study investigated the molecular mechanisms underlying the protective effect of tARPC on renal epithelium during cisplatin nephrotoxicity. By performing a whole-genome transcriptomic analysis, we found that tARPC, in presence of cisplatin, can strongly influence the gene expression of renal proximal tubular cell [RPTEC] by inducing overexpression of CYP1B1, a member of the cytochrome P450 superfamily capable of metabolizing cisplatin and of hypoxia/cancer-related lncRNAs as MIR210HG and LINC00511. Particularly, tARPC exerted renoprotection and regeneration effects via extracellular vesicles (EV) enriched with CYP1B1 and miR-27b-3p, a well-known CYP1B1 regulatory miRNA. The expression of CYP1B1 by tARPC was confirmed by analyzing biopsies of cisplatin-treated renal carcinoma patients that showed the colocalization of CYP1B1 with the tARPC marker CD133. CYP1B1 was also overexpressed in urinary EV purified from oncologic patients that presented nephrotoxicity episodes after cisplatin treatment. Interestingly CYP1B1 expression significantly correlated with creatinine and eGFR levels. Taken together, our results show that tARPC are able to counteract cisplatin-induced nephrotoxicity via CYP1B1 release through EV. These findings provide a promising therapeutic strategy for nephrotoxicity risk assessment that could be related to abundance of renal progenitors.