bluuebunny/crossref_metadata_2025 huggingface dataset:
miR-1, miR-133a, and miR-29b and Skeletal Muscle Fibrosis in Chronic Limb-Threatening Ischaemia.
Authors: Keane Alan J , Sanz-Nogués Clara , Jayasooriya Dulan , Creane Micahel , Chen Xiche , Lyons Caomhán J , Sikri Isha , Goljanek-Whysall Katarzyna , O'Brien Timothy
Published: February 2024

Background. Chronic limb-threatening ischaemia (CLTI) is the most severe manifestation of peripheral arterial disease (PAD), and is associated with poor prognosis and high amputation rates. Novel therapeutic approaches are currently being investigated, but to date, they have not demonstrated significant benefits in promoting CLTI limb salvage. Understanding the molecular pathways of skeletal muscle dysfunction in CLTI is imperative for a rational design of successful therapeutic approaches. The full spectrum of dysregulated microRNAs (miRNAs) associated with PAD disease stages is currently poorly understood. The purpose of this study was to identify dysregulated miRNA transcripts in gastrocnemius muscle biopsies that were differentially expressed in different PAD cohorts.
Methods. We performed an analysis of a publicly available RNA-sequencing database of PAD cohorts using MIcroRNA ENrichment TURned NETwork(MIENTURNET), a web tool for miRNA-target enrichment and network-based analysis. Following this, we validated the dysregulation of miRNAs and their targets in mice with hindlimb ischaemia (HLI).
Results. Our miRNA-target interaction enrichment analysis identified a list of miRNAs over-represented amongst upregulated differentially expressed genes (DEGs) in CLTI. Upon validation in the HLI model, our results showed a significant and marked ischaemia-induced decrease of miR-1, miR-133a, and miR-29b in the ischaemic limbs vs the contralateral non-ischaemic limbs. A miR-1, miR-133a, and miR-29b target protein-protein interaction network identified many extracellular matrix components including collagen-1a1, -3a1 and -4a1, fibronectin-1, fibrin-1, matrix metalloproteinase-2 and -14, and the secreted protein acidic and rich in cysteine (Sparc), which were upregulated in the ischaemic muscle in mice. Functional enrichment analysis performed on this subset of DEGs identified pathways related to fibrosis and vascular pathology.
Conclusions. We have identified, for the first time, a CLTI-specific miRNA signature. Our results indicate miR-1, miR-133a, and miR-29b as potential contributors to the fibrosis and vascular pathology in CLTI muscle, which supports its investigation as potential novel therapeutics.
Global green finance: deaths from air pollution and democracy index
Authors: Lucchetta Marcella
Published: February 2024

The condition of our planet's environment is deteriorating, which is creating difficulties for all living beings on Earth. However, there is potential for many prosperous nations to take more action to fight against climate change, as suggested by Lucchetta in 2023. It's worth questioning why the implementation of climate policies is not being expedited. This study indicates that: a) the level of democracy in a country has a negative correlation with green investments, which implies that it plays a significant role (Farzin and Bond 2006); b) investing in green bonds can reduce deaths caused by CO2 emissions; c) the study provides statistics and comparisons between China, USA, Europe, and Arab countries.

The methology utilized unbalanced panel data from 1985 to the present for the United States and Europe and from 1990 onwards for the United Arab Emirates and China. The data was analyzed using the Generalized Least Square (GLS) method.
On the Topology $\tau_{R}^{\diamond}$ of Primal Topological Spaces
Authors: ÖZKOÇ Murad , KÖSTEL Büşra
Published: February 2024

The main purpose of this paper is to introduce and study two new operators $(\cdot)_{R}^{\diamond}$ and $cl_{R}^{\diamond}(\cdot)$ via primal which is a new notion. We also show that the operator $cl_{R}^{\diamond}(\cdot)$ is a Kuratowski closure operator, while the operator $(\cdot)_{R}^{\diamond}$ is not. In addition, we prove that the topology on $X$, shown as $\tau_{R}^{\diamond}$, obtained by means of the operator $cl_{R}^{\diamond}(\cdot)$ is finer than $\tau_{\delta}$, where $\tau_{\delta}$ is the family of $\delta$-open subsets of a space $(X,\tau)$. Moreover, we not only obtain a base for the topology $\tau_{R}^{\diamond}$ but also prove many fundamental results concerning this new structure. Furthermore, we give many counterexamples related to our results.
2020 AMS Classifications: 54A05; 54B99; 54C60.
Новая коронавирусная инфекция COVID-19 у пациентов с волосатоклеточным лейкозом
Authors: Пластинина Любовь Васильевна , Аль-Ради Л. С. , Моисеева Т. Н. , Костина И. Э. , Чабаева Ю. А. , Барях Е. А. , Желнова Е. И. , Туполева Т. А.
Published: February 2024

Aim. To analyze the characteristics of novel coronavirus infection COVID-19 in hairy cell leukemia (HCL) patients and to assess humoral immune response in those of them who had COVID-19 and/or were vaccinated against SARS-CoV-2.

Materials & Methods. This paper documents the retrospective analysis of the data from 118 HCL patients followed-up at the National Research Center for Hematology from March 2020 to October 2021. Within this period, 99 out of 118 patients had novel coronavirus infection COVID-19; 17 out of 99 COVID-19 patients were vaccinated against SARS-CoV-2 (3 of them prior to being infected and 14 of them after that). The rest of 19 HCL patients without COVID-19 were vaccinated against SARS-CoV-2 during the period from January to October 2021. By the time of HCL diagnosis, the patients were aged 23–74 years (median 47 years), whereas by the time of COVID-19 diagnosis, the age range was 25–82 years (median 55 years). There were 63 men and 36 women.

Results. Severe CT 3–4 lung lesions were diagnosed in 22 (22 %) patients and detected more often (55 %) prior to therapy for advanced-stage HCL. In patients on therapy for advanced-stage HCL and those in remission, the rate of severe lung lesions (CT 3–4) was 17 % in each group. COVID-19 therapy was administered to 49 (49 %) patients in a special hospital. Mortality in the total cohort was 6 % (n = 6) and differed in patients with advanced-stage HCL (n = 4) and those in remission (n = 2). The patients who received anti-CD20 monoclonal antibodies for < 12 months prior to COVID-19 infection, in 42 % (n = 5) of cases showed seronegativity vs. 5 % (n = 1) of patients who received the same therapy > 12 months before COVID-19 was diagnosed.

Conclusion. The most severe COVID-19 was observed in chemotherapy-free patients with advanced-stage HCL compared to patients on therapy or in HCL remission. When anti-CD20 monoclonal antibodies were administered for < 12 months prior to COVID-19 diagnosis, the number of patients with negative anti-SARS-CoV-2-IgG immune response increased 15-fold. Mortality in novel coronavirus infection COVID-19 differed at various HCL stages (in remission and at advanced stage).
Unlocking SME investment potential: The determinants of an effective credit guarantee scheme in Morocco
Authors: Kamal Oussouadi , Kenza Cherkaoui
Published: February 2024

This research seeks to identify the factors influencing the investment potential of SMEs following their receipt of government-guaranteed loans. To this end, an empirical methodology based on the statistical analysis of data collected from a representative sample of Moroccan companies was employed. This sample of 335 SMEs that had benefited from loans with government guarantees was selected at random to ensure its relevance to the population of SMEs in Morocco. The methodological approach is based on a regression analysis using the robust least squares (RLS) method. Firm profitability is positively related to higher investment, suggesting that government guarantees should encourage investment by profitable SMEs. Liquidity, repayment capacity and indebtedness at the time of applying for finance do not appear to influence investment. These elements can be improved after financing, which indicates that government guarantees should not penalize SMEs in difficulty. Decision-making maturity has a negative effect on investment, suggesting that young companies and entrepreneurs tend to invest more. A negative correlation is observed between company size and investment, which suggests that government guarantees should be geared towards small SMEs and young companies and entrepreneurs. Finally, managers with a large share of capital invest more, leading us to believe that government guarantees should favor this type of SME.
Impact of foreign trade and foreign direct investment on economic growth: Empirical insights from Nepal
Authors: Kumar Dahal Arjun , Bhattarai Ganesh , Bahadur Budhathoki Prem
Published: February 2024

This study aims to examine the impact of foreign trade and foreign direct investment on Nepal’s long-term economic growth. It uses secondary data from 1989/90 to 2021/22 collected from various economic surveys of the Ministry of Finance of Nepal. Descriptive and explanatory research designs are used in this study. The trace, max-eigen tests, and fully modified least square methods search the long-run co-integration and impact between response and predictor variables. Trace and max-eigen tests consistently point toward the long-run co-integration between dependent (gross domestic product) and independent (import, export, total trade, and foreign direct investment) variables. Exports and imports are found to be negative and statistically significant to explain Nepal’s economic growth. One unit increase in exports results in a 0.748 unit decrease in Nepal’s economic growth. Similarly, total trade volume and foreign direct investment positively impact economic growth. Each unit increase in foreign direct investment results in a 0.0036 unit increase in GDP in Nepal. Foreign trade has a multiplier effect on Nepal’s GDP growth. The 76.35 percent variation in economic growth depends upon total foreign trade volume, exports, imports, and foreign direct investment. To promote sustainable economic growth, policymakers should prioritize policies encouraging increased total foreign trade and foreign direct investment while carefully managing the potential negative impact of excessive reliance on import dynamics.
Innovative Forms of Iron as Essential Trace Element in Dairy Fortification
Authors: Blinov Andrey , Golik Alexey , Gvozdenko Alexey , Serov Alexander , Rekhman Zafar
Published: February 2024

This article introduces a new method for fortification of dairy products. It is based on a synthesis of biologically active complexes of ascorbic acid, trace iron, and such essential amino acids as lysine, phenylalanine, isoleucine, tryptophan, methionine, valine, and threonine. The computer modeling of the complex structure made it possible to define the optimal interaction parameters of the formulation components. The complex of ascorbic acid, iron, and tryptophan demonstrated the most potent antioxidant activity.
Freeze-Dried Mare’s Colostrum
Authors: Timakova Roza , Iliukhina Yulia , Startsev Vadim
Published: February 2024

People do not consume raw mare’s colostrum. However, colostrum powder can be used as a biologically active additive to fortify functional foods. Mare’s colostrum is a unique natural multicomponent and multifunctional product with valuable immunomodulatory and regenerating properties that can be used therapeutically. This research featured optimal technological parameters of freeze-drying in the production of mare’s colostrum powder at a field-to-spoon full-cycle farm that breeds Vyatka horses. Low-pressure freeze-drying affects the quality of colostrum powder and its nutritional profile. No State Standard has been developed for mare’s colostrums so far. In fact, the only currently standardized colostrum in Russia is that of cows. In the absence of normative documents, the research had to rely on Technical Regulations of Customs Union TR CU 033/2013, State Standard GOST R 52975-2008, and Technical Specifications TS 10.51.56-001-0133214532-2021. Domestic sublimators with automatic control proved quite efficient in sublimation and drying of mare’s colostrum. The new technology requires no additional purification stage because mare’s colostrum possesses antimicrobial properties. The resulting colostrum powder appeared to have a remarkably long shelf-life. A further in-depth study is needed to reveal the nutrient composition of mare’s colostrum powder in connection with its immunomodulatory protein complex.
Milk-Containing Ice-Cream with Improved Protein-Carbohydrate and Mineral Composition
Authors: Tvorogova A. , Sitnikova Polina
Published: February 2024

Milk-containing ice-cream is low in fat and non-fat milk solids. To maintain the conventional sensory and technological properties, dry substances have to be restored, including dry milk solids. This article introduces a new formulation of milk-containing ice-cream with an improved protein-carbohydrate and mineral content and the conventional sensory profile. Whey protein concentrates and dry cheese whey served as substitute components. The research involved physical, chemical, microstructural, and thermostatic methods. In the experimental product, 2 % whey protein concentrate and 4 % dry cheese whey brought dry milk solids up to 38 %, which was similar to the control milk ice-cream sample. The dispersion of ice crystals and the thermal and shape stability were also similar to the traditional ice-cream. However, the dynamic viscosity and the temperature of freezer discharge of the experimental mix exceeded those of the control sample by 1.5 times and 0.6 °C, respectively, while the aerating ability and the air-phase dispersion were lower. Whey protein concentrates and dry cheese improved the protein-carbohydrate and mineral composition of the milk-containing ice-cream, as well as made it possible to approximate it to the traditional sensory parameters. A further study is necessary to select flavors that could improve the sensory profile of the milk-containing ice-cream product.
ENSINO DE GÊNEROS DISCURSIVOS A PARTIR DA RETEXTUALIZAÇÃO
Authors: Nascimento Ilderlândio Assis de Andrade , Medeiros Joyce de Araújo
Published: February 2024

A retextualização pode ser concebida como um importante recurso didático no processo de ensino de gêneros discursivos na sala de aula. Partindo dessa perspectiva, merecem atenção as propostas de ensino presentes no Livro Didático - LD. Assim, este estudo objetiva analisar como o recurso da retextualização aparece em um livro didático de língua portuguesa do quinto ano, considerando propostas de ensino de gêneros discursivos. Os pressupostos teóricos que sustentam esta investigação decorrem dos estudos de Bakhtin (2011), Medviédev (2012) e Volóchinov (2017), especificamente no que tange a concepção de linguagem, enunciado e gêneros do discurso. Também dialoga com os estudos de Marcuschi (2001; 2008), Matencio (2002; 2003), Dell’Isola (2007) e outros, que abordam a retextualização como proposta de ensino. Esta pesquisa apresenta uma abordagem qualitativa, pautada numa perspectiva de compreensão de um corpus composto no LD de Língua Portuguesa. Os resultados apontam que o LD, embora não explicite a terminologia retextualização, apresenta propostas, numa linguagem adaptada aos interlocutores/alunos, que mobilizam procedimentos típicos da retextualização.
FORMAÇÃO, POESIA E ROMANTISMO NO HEINRICH VON OFTERDINGEN DE NOVALIS
Authors: Stuart da Silva Priscilla
Published: February 2024

Este artigo faz uma análise da obra Heinrich von Ofterdingen, do Barão de Hardenberg ‒ Novalis. De alguma forma, a ideia geral é mostrar que os gêneros literário, filosófico e poético não apresentam fronteiras tão demarcadas quando se trata da questão da formação humana. E essa era uma ideia da própria escola romântica, que, para Benjamin, representa uma fonte inesgotável de referências durante toda a sua trajetória como filósofo. Por se tratar de um jovem poeta na Idade Média, Heinrich é um personagem desenvolvido no universo mágico do sonho, da interioridade e do devaneio. A “flor azul” é o símbolo da juventude, relacionada à vida do jovem poeta que dá nome à obra. Ela representa um idílio irremediável, uma eterna saudade da unidade e totalidade perdidas no mundo moderno. O personagem herói está constantemente movimentando-se em uma incansável procura, o que traduz o seu processo formativo. Sua formação interior, seu caráter, suas aptidões artísticas e seus sentimentos são transformados em matéria de complexa transformação, amadurecimento ao longo da história, como um romance de formação.
EDUCAÇÃO PRISIONAL E O PROCESSO DE RESSOCIALIZAÇÃO DE INDIVÍDUOS EM PRIVAÇÃO DE LIBERDADE
Authors: Pessoa de Oliveira Silva Daniel Lucas , Lopes Costa Freire Katia Regina
Published: February 2024

Este artigo consiste em adaptação de trabalho de conclusão de curso e versa acerca da Educação Prisional e da ressocialização dos indivíduos em privação de liberdade. Para organizar o quadro teórico e conceitual que deu sustentação à pesquisa, a revisão de literatura contou com artigos científicos disponibilizados no acervo do portal de periódicos CAPES e teve como objetivo contextualizar a temática e sua contribuição. Por meio desse estudo, discutem-se quais princípios embasam as práticas educacionais desenvolvidas nos ambientes prisionais, por meio de aporte teórico e documental, tais como: Foucault (2002), Beccaria (2019), Novo (2021), Freire (1987), a Constituição Federal (Brasil, 1988), a Lei de Execução Penal (Brasil, 1984), entre outros. Destacam-se a ausência da Educação Prisional nos cursos de formação de professores do país e a oferta de uma educação que se distancia muito da proposta freiriana de emancipação, pois visa apenas atender, de forma tecnicista, ao mercado de trabalho ou à redução da pena.
CREB: A Promising Therapeutic Target for Treating Psychiatric Disorders
Authors: Guan Wei , Ni Mei-Xin , Gu Hai-Juan , Yang Yang
Published: February 2024

:

Psychiatric disorders are complex, multifactorial illnesses. It is challenging for us to understand the underlying mechanism of psychiatric disorders. In recent years, the morbidity of psychiatric
disorders has increased yearly, causing huge economic losses to the society. Although some progress,
such as psychotherapy drugs and electroconvulsive therapy, has been made in the treatment of psychiatric disorders, including depression, anxiety, bipolar disorder, obsessive-compulsive and autism spectrum disorders, antidepressants and psychotropic drugs have the characteristics of negative effects and
high rate of relapse. Therefore, researchers continue to seek suitable interventions. cAMP response element binding protein (CREB) belongs to a protein family and is widely distributed in the majority of
brain cells that function as a transcription factor. It has been demonstrated that CREB plays an important role in neurogenesis, synaptic plasticity, and neuronal growth. This review provides a 10-year
update of the 2013 systematic review on the multidimensional roles of CREB-mediated transcriptional
signaling in psychiatric disorders. We also summarize the classification of psychiatric disorders and
elucidate the involvement of CREB and related downstream signalling pathways in psychiatric disorders. Importantly, we analyse the CREB-related signal pathways involving antidepressants and antipsychotics to relieve the pathological process of psychiatric disorders. This review emphasizes that
CREB signalling may have a vast potential to treat psychiatric disorders like depression. Furthermore,
it would be helpful for the development of potential medicine to make up for the imperfection of current antidepressants and antipsychotics.
Protecting collective rights in Colombia
Authors: Gamarra-Amaya Laura Cecilia , Venegas Segura Andrés Arturo , Fino-Puerto Nelson Ricardo
Published: February 2024

The defense of collective rights and the appropriate reparation of damages to victims is a recent development issue in the Colombian legal system. Although some legal provisions mentioned damages to an indeterminate group of people in cases of negligence, it was not until the 1991 Constitution that Colombia established the types of actions all individuals or groups can initiate to advocate for the integrity of their rights in the face of illegal actions or omissions by the State. There is currently no mechanism for groups to sue private entities efficiently that guarantees all individual and collective rights, akin to class actions. This scientific article delves into the multifaceted landscape of collective rights protection in Colombia, focusing specifically on the mechanisms and implications surrounding the deployment of group actions.
The Use of Medical Services for Low-Acuity Emergency Cases in Germany: Protocol for a Multicenter Observational Pilot Study
Authors: Nau Lara Maria , Laux Gunter , Altiner Attila , Szecsenyi Joachim , Leutgeb Rüdiger
Published: February 2024

Background

The increasing number of requests for help for acutely ill patients and their management is a major problem in the health systems of many countries, but especially in Germany. Rescue coordination centers and ambulances in Germany are increasingly overloaded. As a result, rides as a part of rescue operations have been increasing in length for years, yet a relevant proportion of these operations represent low-acuity calls (LACs). The basic objective of this pilot study is the quantitative analysis of the potential misuse of requests to the rescue control center. Indications for alternative treatment options and how to handle these treatment options in nonacute, non–life-threatening health conditions, such as minor injuries or minor infectious diseases, will be assessed. The identification of these LACs is vital in order to prevent health care resources in emergency medical care becoming inadequate.

Objective

The overarching goal of this study is to determine the percentage of unnecessary rescue missions on site and subsequently to obtain an impression of the paramedics’ assessment of alternative treatment options or alternative methods of rescue transportation.

Methods

This will be an exploratory, noninterventional, cross-sectional study with a quantitative approach. The study is multicentric, with 21 ambulances in 12 different locations. The data for this study were collected via a questionnaire, newly developed for this study, for rescue personnel. Additionally, secondary data from the responsible control center will be linked and processed in an initial descriptive analysis. This descriptive analysis will form the basis for a subsequent variance analysis.

Results

Data collection started as projected on September 18, 2023, and was ongoing until end of November 2023. We expect the documentation of several thousand rescue operations. We expect the following study results: (1) many unnecessary rescue operations, (2) immediate on-site assessment of correct care and treatment, and (3) patients’ reasons for calling a rescue coordination center.

Conclusions

To our knowledge, this is the first observational study in which acute rescue operations are recorded on site. The focus of this study is on the trained paramedics’ assessment of whether rescue operations are necessary or not. Additionally, alternative treatments, such as out-of-hours care service or primary care service, are shown for each individual case. The study also intends to cover the question of which factors are relevant and statistically significantly connected to the misuse of ambulances.

Trial Registration

German Register for Clinical Studies (Deutsches Register für Klinische Studien) DRKS00032510; https://drks.de/search/en/trial/DRKS00032510

International Registered Report Identifier (IRRID)

DERR1-10.2196/54002
Developing a Multiprofessional Mobile App to Enhance Health Habits in Older Adults: User-Centered Approach
Authors: Sobrinho Andressa Crystine da Silva , Gomes Grace Angelica de Oliveira , Bueno Júnior Carlos Roberto
Published: February 2024

Background

Although comprehensive lifestyle habits are crucial for healthy aging, their adherence tends to decline as individuals grow older. Sustaining a healthy life over time poses a motivational challenge. Some digital tools, such as smartphone apps aimed at promoting healthy habits, have been used to counteract this decline. However, a more profound investigation is necessary into the diverse experiences of users, particularly when it concerns older adults or those who are unfamiliar with information and communications technologies.

Objective

We aimed to develop a mobile app focused on promoting the health of older adults based on the principles of software engineering and a user-centered design. The project respected all ethical guidelines and involved the participation of older adults at various stages of the development of the app.

Methods

This study used a mixed methods approach, combining both quantitative and qualitative methodologies for data collection. The study was conducted in Ribeirão Prêto, São Paulo, Brazil, and involved 20 older adults of both genders who were aged ≥60 years and enrolled in the Physical Education Program for the Elderly at the University of São Paulo. The research unfolded in multiple phases, encompassing the development and refinement of the app with active engagement from the participants.

Results

A total of 20 participants used a mobile health app with an average age of 64.8 (SD 2.7) years. Most participants had a high school education, middle-class status, and varying health literacy (mean score 73.55, SD 26.70). Overall, 90% (18/20) of the participants owned smartphones. However, 20% (4/20) of the participants faced installation challenges and 30% (6/20) struggled with web-based searches. The focus groups assessed app usability and satisfaction. Adjustments increased satisfaction scores significantly (Suitability Assessment of Materials: 34.89% to 70.65%; System Usability Scale: 71.23 to 87.14). Participant feedback emphasized font size, navigation, visual feedback, and personalization, and suggestions included health device integration, social interaction, and in-app communication support.

Conclusions

This study contributes to the development of health care technologies tailored to the older adult population, considering their specific needs. It is anticipated that the resulting app will serve as a valuable tool for promoting healthy habits and enhancing the quality of life for older adults.
Deepfake technology amidst health emergencies: Discussing challenges, exploring opportunities, and charting strategic future paths (Preprint)
Authors: UNISA Walter
Published: February 2024

BACKGROUND

This study proposes strategic directions for future research and policymaking, fostering a nuanced understanding of the dynamic interplay between deepfake technology and health emergencies. It provides recommendations for policymakers and stakeholders to effectively navigate challenges and harness opportunities presented by deepfake technology.

OBJECTIVE

_The primary objective is to provide insights into the applications of deepfake technology during health emergencies, analysing its effects on information dissemination, public trust, and crisis management_

METHODS

This paper employs a comprehensive approach to investigate the impact of deepfake technology in the context of health emergencies. The study examines challenges, opportunities, and strategic pathways for the future.

RESULTS

Challenges identified include the potential for misinformation, erosion of trust in health communication, and disruptions to established rapid response mechanisms. Opportunities exist to utilise deepfake technology for medical training and health awareness campaigns.

CONCLUSIONS

The paper contributes original insights by evaluating current detection and prevention strategies, acknowledging advancements, and recognising limitations. It offers a comprehensive discussion on the broader implications of deepfakes for emergency health management.
Comparing In-Person, Standard Telehealth, and Remote Musculoskeletal Examination with a Novel Augmented Reality Exercise Game System: A Pilot Study (Preprint)
Authors: Wu Richard , Chakka Keerthana , Belko Sara , Khargonkar Ninad , Desai Kevin , Prabhakaran Balakrishnan , Annaswamy Thiru
Published: February 2024

BACKGROUND

Current telemedicine technology is not optimized for conducting physical examinations. The Virtual Remote Tele-Physical Examination (VIRTEPEX) system, a novel proprietary technology platform which uses a Microsoft Kinect-based augmented reality game system to track motion and estimate force, has potential to assist with conducting asynchronous, remote musculoskeletal examination.

OBJECTIVE

This pilot study evaluates the feasibility of the VIRTEPEX system for supplementing telehealth musculoskeletal strength assessments.

METHODS

In this cross-sectional pilot study, 12 study participants with upper extremity pain and/or weakness underwent in-person, telehealth, VIRTEPEX, and composite (telehealth plus VIRTEPEX) strength evaluations for four upper extremity movements. Evaluators were blinded to one another’s evaluations. The primary outcome was feasibility, as determined by participant study recruitment, completion, and safety. The secondary outcome was preliminary assessment of inter-rater agreements between in-person, telehealth, and VIRTEPEX strength assessments, plus kappa statistical calculations.

RESULTS

This pilot study had an 80% recruitment rate, 100% completion rate, and 0 adverse events. In-person and telehealth evaluations had highest overall agreement (85.71%), greater than the agreements between in-person and VIRTEPEX (62.50%), in-person and composite (75%), and telehealth and VIRTEPEX evaluations (62.50%). However, for shoulder flexion, agreement between in-person and VIRTEPEX (78.57%, κ=0.571, 95% CI of 0.183 to 0.960), and also in-person and composite evaluations (78.57%, κ=0.571, 95% CI of 0.183 to 0.960), was greater than that for in-person and telehealth (71.43%, κ=0.429, 95% CI of -0.025 to 0.882).

CONCLUSIONS

This work demonstrates the feasibility of asynchronous VIRTEPEX examination and supports the potential for VIRTEPEX to supplement and add value to standard telehealth platforms. Further study, with additional development of VIRTEPEX and larger sample size for adequate power, is warranted.
Trends in Automated Peritoneal Dialysis Prescriptions in a Large Dialysis Organization in the United States
Authors: Giles Harold E. , Parameswaran Vidhya , Lasky Rachel , Ficociello Linda H. , Mullon Claudy , Chatoth Dinesh K. , Kraus Michael , Anger Michael S.
Published: February 2024

Key Points

This is the largest analysis of incident automated peritoneal dialysis (PD) prescriptions conducted in the United States to date.

There was limited variability of automated PD prescriptions across the first 4 months of therapy.

PD prescriptions tailored to meet the dialysis needs and lifestyle of patients may make PD a more attractive choice and increase longevity on PD.

Background

Changes in health care policies and recognition of patient benefit have contributed to increases in home-based dialysis, including peritoneal dialysis (PD). Frequent monitoring and early individualization of PD prescriptions are key prerequisites for the delivery of high-quality PD. The present analysis aimed to assess variations in PD prescriptions among incident automated PD (APD) patients who remain on PD for 120+ days.

Methods

This retrospective analysis examined data from patients within a large dialysis organization that initiated PD with APD between 2015 and 2019. PD prescription data were described by calendar year, timing of PD, and residual renal function categories. Changes in prescriptions from PD initiation (day 1) to day 120 were assessed descriptively.

Results

The cohort included 11,659 patients. The mean age at PD initiation increased from 2015 (56 [15] years) through 2019 (58 [15] years), whereas most other variables demonstrated no clear temporal change. Most patients (86%) had nighttime PD prescribed, with an average of 4.9 (1.3) cycles per day, a mean total treatment volume of 9.3 (2.5) L, and a median daily total dwell time of 7 (6–9.5) hours. Relative to day 1 nighttime prescriptions, there were (1) small increases in the proportion of patients receiving three or fewer cycles per day and those receiving 6+ cycles per day, (2) a 100 ml mean increase in fill volume per exchange, and (3) a mean 0.5 L increase in total nighttime treatment volume at day 120. When changes in nighttime APD prescriptions were examined at the patient level, 49% of patients had day 120 prescriptions that were unchanged from their initial prescription.

Conclusions

In the largest analysis of incident APD prescriptions conducted in the United States to date, most patients were prescribed nocturnal PD only with limited variability across the first 4 months of therapy.
Pedagogical Journey of Pre-service Teachers: Stories to Tell
Authors: Galut Mark Neil A.
Published: February 2024

This research aimed to identify the experiences, coping mechanisms, and insights of nine Apayao State College pre-service teachers during their internship. The study used purposive convenience sampling and in-depth interviews with nine graduates. The results showed that pre-service teachers faced challenges, managed them through coping mechanisms, and shared their insights about their pedagogical journey. The study recommends workshops, proper orientation, and regular conferences for learning development, emphasizing the importance of sharing experiences and fostering professional growth among pre-service teachers.
Application Research of Fish-Plant Symbiosis Technology in a Urban Lake: A Case Study of the Environment Remediation Project with Machine Learning Application
Authors: Chen Xi , Lu Dunxian , Chen Tao , Liu Ti , Luo Yijuan , Liu Jing , Wei Shengjie , Liu Ying
Published: February 2024

This research examines the Jiefang Park Water Environment Remediation Project, a pioneering initiative in urban lake rehabilitation. The project is anchored in a fish-plant symbiosis system, which has significantly improved water quality, upgrading it from Category V to Category III, and in certain zones, achieving the commendable Category II. This enhancement is quantitatively marked by a notable reduction in nitrogen and phosphorus levels, and a marked increase in water transparency, with submerged plants covering over 70% of the lakebed.A key innovation is the utilization of a machine learning model to predict chlorophyll-a concentration, a vital water quality metric. The model’s accuracy is underscored by its R² values, ranging from 0.23 to 0.99, and RMSE values between 6.921 and 0.237, with the best performance at point 1. Additionally, comprehensive measurement data highlight the project’s effectiveness. Water transparency significantly improved from baseline levels, as evidenced by increased dissolved oxygen levels at various sampling sites, indicative of restored ecological equilibrium in the lake. This study represents an exemplary fusion of ecological restoration and advanced data analytics, signifying a substantial advancement in urban lake restoration. It underscores the potential of combining synergistic ecological approaches with technological innovation to further sustainable urban environmental management.
Teaching Academic English with the Production-Oriented Approach: A Case Study
Authors: MA Yingwei
Published: February 2024

This paper aims to explore how to apply POA to EAP teaching in China and test its effectiveness through a case study. Three key issues are discussed: the design of the course structure of EAP under the guidance of POA, the implementation of POA in teaching academic English and the effectiveness of POA in increasing of students’ academic English proficiency. It is found that the ideal framework of Academic English consisted of integrated modules, instead of a linear arrangement of the units. Three key factors in the POA teaching process include: motivating tasks in an academic setting with both communicative and linguistic challenges, enabling tasks with corresponding scaffolding and evaluating strategies, and a combination of teacher’s evaluation and peer review with co-produced rubrics for assessment. The results of the study demonstrate the efficacy of the production-oriented approach in enhancing students’ academic English proficiency, specifically in the areas of speaking and writing.
Empowering Farmers for Economic Resilience with Agrinet’s Comprehensive Solutions
Authors: Gadakh Aditi
Published: February 2024

Abstract: In the realm of contemporary agriculture, where farmers grapple with multifaceted challenges such as unpredictable weather patterns and market fluctuations, our innovative solution stands out as a beacon of empowerment and resilience. We've seamlessly integrated cutting-edge technology with centuries of farming wisdom, fundamentally reshaping agricultural practices. Our comprehensive approach includes Native Language Support, breaking down linguistic barriers to ensure universal access to critical information. Our advanced weather forecasting equips farmers with up-to-the-minute meteorological data, enabling informed decisions and mitigating weather-related losses. Furthermore, our platform streamlines access to government support, enhancing overall farm productivity and sustainability. Additionally, our interactive discussion forum fosters knowledge sharing and mutual support, nurturing a sense of solidarity among farmers. In the complex fabric of agriculture, our mission is to furnish farmers with holistic tools, knowledge, and a robust community network, ushering in an era marked by prosperity, sustainability, and unwavering resilience in navigating the evolving agricultural landscape
An Analytical Study on Effectiveness of Advertising on Branding
Authors: Rai Dr. Supriya
Published: February 2024

Abstract: This research paper delves into the profound influence of effective advertising on the process of branding. Advertising serves as a pivotal tool in shaping the brand identity, perception, and recognition in the minds of consumers. The paper reviews various methods to assess advertising effectiveness, including quantitative and qualitative metrics, tracking consumer engagement, and analyzing market trends. By scrutinizing real-world examples of brands that have effectively harnessed advertising to reinforce their brand image, the research underscores the symbiotic relationship between compelling advertisements and the creation of enduring brand equity. Furthermore, the paper discusses the potential challenges and ethical considerations that arise when employing advertising to build and enhance brands. It investigates the balance between creating impactful, memorable ads and maintaining authenticity and transparency in brand messaging.
Management of Patients with Oral Lesions Associated Systemic Lupus Erythematous (SLE) Nephritis: A Case Report
Authors: Nur’aeny Nanan , Harmiyati Rahmi
Published: February 2024

Introduction: Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disease involving multi-organ such as mucocutaneous, musculoskeletal, and blood vessels with varied extraoral and intraoral clinical manifestations that require treatment from many medical specialists including dentists. The purpose of writing a case report is to describe how to manage oral lesions in patients with SLE Nephritis.

Case report: A female 17 years old patient was referred to the oral medicine department of Dr. Hasan Sadikin General Hospital from the pediatrics department and was diagnosed with SLE nephritis due to complaints of ulcers on almost the entire oral mucosa. Extraoral examination revealed a moon face, malar rash, and erythematous rash on the palms of the hands. Intraoral examination revealed multiple ulcers, on labial mucosa, buccal mucosa, lateral right of the tongue, palate, and white plaque on the dorsum of the tongue. The therapy provided was non-pharmacological and pharmacological. Non-pharmacological therapies include oral hygiene instruction and communication, information, education.  Pharmacological therapy is a solution containing sucralfate and prednisone which is gargled for one minute, then followed by nystatin oral suspension drug that discards after one minute, then followed by chlorhexidine digluconate 0,12% mouthwash, gargle for one minute, as an antiseptic (each drug is applied in 0,5 – 1 hours). The patient has received a systemic corticosteroid drug (methylprednisolone), but the treatment of oral lesions requires additional topical corticosteroids to accelerate the healing process because of its direct effect on the mucosa.

Conclusion: The management of oral lesions using topical corticosteroids in patients with SLE nephritis can help accelerate the healing process.

Keywords: Management, Oral Lesions, SLE nephritis
Exploring the Therapeutic Potential of Chrysopogon zizanioides Essential Oil: A Comprehensive Study on Its Chemical Composition, Thermal Analysis, and Biological Activities
Authors: Jha Vikas , Marick Arpita , Bhargava Agraj , Thakur Kabir , Jhangiani Ashish , Jagtap Leena , Parulekar Omkar , Joshi Hricha , Pingale Devyani , Chidrala Sakshi
Published: February 2024

Natural bioactive compounds sourced from plants and plant parts have become integral to drug discovery as natural products. The overreliance on synthetic drugs has adversely affected human health. This study focuses on the essential oil from Chrysopogon zizanioides, commonly known as "Vetiver" to explore its medicinal value. The oil underwent various analytical and biological assessments. Extraction involved hydro-distillation of the roots, and GC-MS analysis unveiled its composition. FAME analysis provided insights into fatty acid composition. Infrared spectroscopy identified different functional groups. Thermal stability was gauged using TG-DTA and DSC methods. The essential oil's antibacterial potency was tested against bacterial strains. Antimalarial and antituberculosis potential were evaluated against Plasmodium falciparum and Mycobacterium tuberculosis, respectively. Vetiver essential oil's ability to counter free radicals was determined via the DPPH reagent. Its impact on biofilm produced by 5 cultures was studied. The essential oil's effects on cancerous (HeLa) and normal (CHO) cell lines were assessed using the MTT assay. In conclusion, Chrysopogon zizanioides essential oil exhibits considerable potential with diverse activities. It could serve as a non-toxic alternative for infection prevention and find utility in the food industry

Keyword: Chrysopogon zizanioides, Essential oil, Thermal Characterization, GCMS, Antimicrobial, Biofilm.
Recurrence of Diabetic Foot Ulcer and Associated Risk Factors: A 3-Year Retrospective Study
Authors: Oham Maryann Kanayo , Abonyi Michael Chinweuba , Ugwu Theophilus Ejiofor , Ezeude Chidiebele Malachy , Okechukwu Uzoma Chukwunonso , Ekochin Fintan Chinweike , Maluze John Aniebo , Ugwuaku Obiageli , Ahanonu Ifeoma Mercy , Ugwu Nkemdilim Justina , Okorie Nnenna Nkemjika
Published: February 2024

The burden of diabetes foot ulcer is immense, resulting to prolonged hospital stay and high cost of care. The aim of this study is to identify the predictors of ulcer recurrence which will help mitigate this disabling, pocket-draining, but highly preventable complication of diabetes mellitus (DM).

Methodology: This was a 3-year retrospective study of patients hospitalised for diabetic foot ulcer (DFU) in Enugu State University Teaching Hospital from March 2020 to February 2023. Information on demographics, relevant diabetes history and complications, characteristics of DFU, outcome, DFU recurrence were obtained.

Results: Most of the patients (82.5%) were between 41 and 70 years of age with a mean age is 57.86 ± 12.45. There were marginally more females (52.6%) than male (47.4%). The subjects were predominantly businessmen/traders (43.9%), and 73.7% had DM duration of 1-5 years. Duration of admission was 8 weeks for 31.6% of the patients, 4 weeks for 26.3%, 3 weeks for 21.1% and 12 weeks for 12.3% of the patients. More of the patients were in Wagner grades 3 (43.9%) and 4 (31.6%). The Prevalence of (diabetic peripheral neuropathy) DPN, peripheral artery disease (PAD) and Retinopathy among the patients were 82.5%, 45.6% and 59.6% respectively. Recurrence of DFU occurred in 35% of the subjects. Occupation and wound site positively correlated with reocurrence in this study. The outcome shows that 59.6% of the patients had good healing, 21.1% had ray amputation while 14% had below knee amputation.

Keywords: Diabetic foot ulcer, recurrence, peripheral neuropathy, peripheral artery disease.
Experimental Animal Models for Influenza/Flu Virus Vaccine Development
Authors: Gupta Ayushi , Satapathy Trilochan , Pradhan Bharti , Sen Kalpana , Sahu Shailesh , Sahu Akhilesh Kumar , Bhardwaj Shiv Kumar , Khan Md. Altaf
Published: February 2024

Objectives: The objective of this review is to explore the different animal models used in the development of Influenza / Flu Virus Vaccine as well as some important guidelines adhered to it.

Methods: Laboratory animal models are widely used in the preclinical evaluation of potential vaccines and antiviral compounds to investigate the safety and efficacy of the vaccine or compound in preventing or moderating infection, disease, or secondary transmission. Animal models must also represent humans in terms of similarity of clinical signs, histopathological changes, virus growth kinetics, or transmission. Common animal models for influenza virus vaccine testing include mice, ferrets, and non-human primates. These models help researchers assess vaccine efficacy, immune response, and potential side effects before advancing to human trials. Animal models play a crucial role in the development and testing of influenza vaccines, as they help researchers examine potential side effects and adverse reactions, evaluate the efficacy of vaccines, study the immune response elicited by vaccines, understand the pathogenesis of influenza virus infection, and predict the potential impact of emerging strains. They also help test various vaccine platforms and delivery methods, and compare the effectiveness of different vaccine formulations in animals to identify promising candidates for further human trials.

Results: We have discussed in detail the various animal models such as Chick embryo model for the development of influenza virus vaccine and mice, ferrets, guinea pig and non-human primates etc. used for the evaluation of safety and therapeutic effectiveness.

Conclusion: As we have discussed various animal models, their merits, demerits etc. for the evaluation of safety and effectiveness of influenza virus vaccine and expect that these will help for the young researchers to carry out their vaccine research using suitable animal models.

Keywords: influenza virus, vaccine development, mouse models, ferret model, guinea pig model, cotton rat model