bluuebunny/crossref_metadata_2025 huggingface dataset:
Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics
Authors: Kaloyianni Martha , Bobori Dimitra C. , Xanthopoulou Despoina , Malioufa Glykeria , Sampsonidis Ioannis , Kalogiannis Stavros , Feidantsis Konstantinos , Kastrinaki Georgia , Dimitriadi Anastasia , Koumoundouros George , Lambropoulou Dimitra A. , Kyzas George Z. , Bikiaris Dimitrios N.
Published: November 2021

Microplastics (MPs)’ ingestion has been demonstrated in several aquatic organisms. This process may facilitate the hydrophobic waterborne pollutants or chemical additives transfer to biota. In the present study the suitability of a battery of biomarkers on oxidative stress, physiology, tissue function and metabolic profile was investigated for the early detection of adverse effects of 21-day exposure to polystyrene microplastics (PS-MPs, sized 5–12 μm) in the liver and gills of zebrafish Danio rerio and perch, Perca fluviatilis, both of which are freshwater fish species. An optical volume map representation of the zebrafish gill by Raman spectroscopy depicted 5 μm diameter PS-MP dispersed in the gill tissue. Concentrations of PS-MPs close to the EC50 of each fish affected fish physiology in all tissues studied. Increased levels of biomarkers of oxidative damage in exposed fish in relation to controls were observed, as well as activation of apoptosis and autophagy processes. Malondialdehyde (MDA), protein carbonyls and DNA damage responses differed with regard to the sensitivity of each tissue of each fish. In the toxicity cascade gills seemed to be more liable to respond to PS-MPs than liver for the majority of the parameters measured. DNA damage was the most susceptible biomarker exhibiting greater response in the liver of both species. The interaction between MPs and cellular components provoked metabolic alterations in the tissues studied, affecting mainly amino acids, nitrogen and energy metabolism. Toxicity was species and tissue specific, with specific biomarkers responding differently in gills and in liver. The fish species that seemed to be more susceptible to MPs at the conditions studied, was P. fluviatilis compared to D. rerio. The current findings add to a holistic approach for the identification of small sized PS-MPs’ biological effects in fish, thus aiming to provide evidence regarding PS-MPs’ environmental impact on wild fish populations and food safety and adequacy.
Gut–Kidney Axis on Chip for Studying Effects of Antibiotics on Risk of Hemolytic Uremic Syndrome by Shiga Toxin-Producing Escherichia coli
Authors: Lee Yugyeong , Kim Min-Hyeok , Alves David Rodrigues , Kim Sejoong , Lee Luke P. , Sung Jong Hwan , Park Sungsu
Published: November 2021

Shiga toxin-producing Escherichia coli (STEC) infects humans by colonizing the large intestine, and causes kidney damage by secreting Shiga toxins (Stxs). The increased secretion of Shiga toxin 2 (Stx2) by some antibiotics, such as ciprofloxacin (CIP), increases the risk of hemolytic–uremic syndrome (HUS), which can be life-threatening. However, previous studies evaluating this relationship have been conflicting, owing to the low frequency of EHEC infection, very small number of patients, and lack of an appropriate animal model. In this study, we developed gut–kidney axis (GKA) on chip for co-culturing gut (Caco-2) and kidney (HKC-8) cells, and observed both STEC O157:H7 (O157) infection and Stx intoxication in the gut and kidney cells on the chip, respectively. Without any antibiotic treatment, O157 killed both gut and kidney cells in GKA on the chip. CIP treatment reduced O157 infection in the gut cells, but increased Stx2-induced damage in the kidney cells, whereas the gentamycin treatment reduced both O157 infection in the gut cells and Stx2-induced damage in the kidney cells. This is the first report to recapitulate a clinically relevant situation, i.e., that CIP treatment causes more damage than gentamicin treatment. These results suggest that GKA on chip is very useful for simultaneous observation of O157 infections and Stx2 poisoning in gut and kidney cells, making it suitable for studying the effects of antibiotics on the risk of HUS.
The Role of Pseudomonas aeruginosa Virulence Factors in Cytoskeletal Dysregulation and Lung Barrier Dysfunction
Authors: Wagener Brant M. , Hu Ruihan , Wu Songwei , Pittet Jean-Francois , Ding Qiang , Che Pulin
Published: November 2021

Pseudomonas (P.) aeruginosa is an opportunistic pathogen that causes serious infections and hospital-acquired pneumonia in immunocompromised patients. P. aeruginosa accounts for up to 20% of all cases of hospital-acquired pneumonia, with an attributable mortality rate of ~30–40%. The poor clinical outcome of P. aeruginosa-induced pneumonia is ascribed to its ability to disrupt lung barrier integrity, leading to the development of lung edema and bacteremia. Airway epithelial and endothelial cells are important architecture blocks that protect the lung from invading pathogens. P. aeruginosa produces a number of virulence factors that can modulate barrier function, directly or indirectly, through exploiting cytoskeleton networks and intercellular junctional complexes in eukaryotic cells. This review summarizes the current knowledge on P. aeruginosa virulence factors, their effects on the regulation of the cytoskeletal network and associated components, and molecular mechanisms regulating barrier function in airway epithelial and endothelial cells. A better understanding of these processes will help to lay the foundation for new therapeutic approaches against P. aeruginosa-induced pneumonia.
Cosmological Model with Interconnection between Dark Energy and Matter
Authors: Bisnovatyi-Kogan Gennady S.
Published: November 2021

It is accepted in the present cosmology model that the scalar field, which is responsible for the inflation stage in the early universe, transforms completely into matter, and the accelerated universe expansion is presently governed by dark energy (DE), whose origin is not connected with the inflationary scalar field. We suppose here that dark matter (DM) has a common origin with a small variable component of dark energy (DEV). We suggest that DE may presently have two components, one of which is the Einstein constant Λ, and another, smaller component DEV (ΛV) comes from the remnants of the scalar field responsible for inflation, which gave birth to the origin of presently existing matter. In this note we consider only the stages of the universe expansion after recombination, z≃1100, when DM was the most abundant component of the matter, therefore we suggest for simplicity that a connection exists between DM and DEV so that the ratio of their densities remains constant over all the stages after recombination, ρDM=αρDEV, with a constant α. One of the problems revealed recently in cosmology is a so-called Hubble tension (HT), which is the difference between values of the present Hubble constant, measured by observation of the universe at redshift z≲1, and by observations of a distant universe with CMB fluctuations originated at z∼1100. In this paper we suggest that this discrepancy may be explained by deviation of the cosmological expansion from a standard Lambda-CDM model of a flat universe, due to the action of an additional variable component DEV. Taking into account the influence of DEV on the universe’s expansion, we find the value of α that could remove the HT problem. In order to maintain the almost constant DEV/DM energy density ratio during the time interval at z<1100, we suggest the existence of a wide mass DM particle distribution.
Application of Multi-Criteria Decision-Making Model and Expert Choice Software for Coastal City Vulnerability Evaluation
Authors: Bagheri Milad , Zaiton Ibrahim Zelina , Mansor Shattri , Abd Manaf Latifah , Akhir Mohd Fadzil , Talaat Wan Izatul Asma Wan , Beiranvand Pour Amin
Published: November 2021

Climate change is regarded as a serious threat to both environment and humanity, and as a result, it has piqued worldwide attention in the twenty-first century. Natural hazards are expected to have major effects in the coastal cities of the globe. At the same time, about two-thirds of the world’s human population lives in the coastal margins. One of the fundamental issues for coastal city planners is the coastal cities’ environmental change. This paper presents the application of a model framework for the management and sustainable development of coastal cities under a changing climate in Kuala Terengganu Malaysia. The analytic hierarchy process (AHP) is performed in the Expert Choice software for coastal city hazard management. This approach enables decision-makers to evaluate and identify the relative priorities of vulnerability and hazard criteria and sub-criteria based on a set of preferences, criteria, and alternatives. This paper also presents a hierarchy erosion design applied in Kuala Terengganu to choose the important sustainable weights of criteria and sub-criteria as well as the zone as an alternative model.
Dose-Dependent Outcome of EBV Infection of Humanized Mice Based on Green Raji Unit (GRU) Doses
Authors: Chen Haiwen , Zhong Ling , Zhang Wanlin , Zhang Shanshan , Hong Junping , Zhou Xiang , Zhang Xinyu , Feng Qisheng , Chen Yixin , Zeng Yi-Xin , Xu Miao , Krummenacher Claude , Zhang Xiao
Published: November 2021

Humanized mouse models are used as comprehensive small-animal models of EBV infection. Previously, infectious doses of EBV used in vivo have been determined mainly on the basis of TD50 (50% transforming dose), which is a time-consuming process. Here, we determined infectious doses of Akata-EBV-GFP using green Raji units (GRUs), and characterized dose-dependent effects in humanized mice. We defined two outcomes in vivo, including an infection model and a lymphoma model, following inoculation with low or high doses of Akata-EBV-GFP, respectively. Inoculation with a low dose induced primary B cells to become lymphoblastoid cell lines in vitro, and caused latent infection in humanized mice. In contrast, a high dose of Akata-EBV-GFP resulted in primary B cells death in vitro, and fatal B cell lymphomas in vivo. Following infection with high doses, the frequency of CD19+ B cells decreased, whereas the percentage of CD8+ T cells increased in peripheral blood and the spleen. At such doses, a small part of activated CD8+ T cells was EBV-specific CD8+ T cells. Thus, GRUs quantitation of Akata-EBV-GFP is an effective way to quantify infectious doses to study pathologies, immune response, and to assess (in vivo) the neutralizing activity of antibodies raised by immunization against EBV.
Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus Reservoir
Authors: Bokelmann Marcel , Vogel Uwe , Debeljak Franka , Düx Ariane , Riesle-Sbarbaro Silke , Lander Angelika , Wahlbrink Annette , Kromarek Nicole , Neil Stuart , Couacy-Hymann Emmanuel , Prescott Joseph , Kurth Andreas
Published: November 2021

Although there have been documented Ebola virus disease outbreaks for more than 40 years, the natural reservoir host has not been identified. Recent studies provide evidence that the Angolan free-tailed bat (Mops condylurus), an insectivorous microbat, is a possible ebolavirus reservoir. To investigate the potential role of this bat species in the ecology of ebolaviruses, replication, tolerance, and persistence of Ebola virus (EBOV) were investigated in 10 different primary bat cell isolates from M. condylurus. Varying EBOV replication kinetics corresponded to the expression levels of the integral membrane protein NPC1. All primary cells were highly tolerant to EBOV infection without cytopathic effects. The observed persistent EBOV infection for 150 days in lung primary cells, without resultant selective pressure leading to virus mutation, indicate the intrinsic ability of EBOV to persist in this bat species. These results provide further evidence for this bat species to be a likely reservoir of ebolaviruses.
Development of a Novel Loop Mediated Isothermal Amplification Assay (LAMP) for the Rapid Detection of Epizootic Haemorrhagic Disease Virus
Authors: Rajko-Nenow Paulina , Howson Emma L. A. , Clark Duncan , Hilton Natasha , Ambagala Aruna , Svitek Nicholas , Flannery John , Batten Carrie
Published: November 2021

Epizootic haemorragic disease (EHD) is an important disease of white-tailed deer and can cause a bluetongue-like illness in cattle. A definitive diagnosis of EHD relies on molecular assays such as real-time RT-qPCR or conventional PCR. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a cost-effective, specific, and sensitive technique that provides an alternative to RT-qPCR. We designed two sets of specific primers targeting segment-9 of the EHD virus genome to enable the detection of western and eastern topotypes, and evaluated their performance in singleplex and multiplex formats using cell culture isolates (n = 43), field specimens (n = 20), and a proficiency panel (n = 10). The limit of detection of the eastern and western RT-LAMP assays was estimated as ~24.36 CT and as ~29.37 CT in relation to real-time RT-qPCR, respectively, indicating a greater sensitivity of the western topotype singleplex RT-LAMP. The sensitivity of the western topotype RT-LAMP assay, relative to the RT-qPCR assay, was 72.2%, indicating that it could be theoretically used to detect viraemic cervines and bovines. For the first time, an RT-LAMP assay was developed for the rapid detection of the EHD virus that could be used as either a field test or high throughput screening tool in established laboratories to control the spread of EHD.
Characterization of Foot-and-Mouth Disease Viruses in Zambia-Implications for the Epidemiology of the Disease in Southern Africa
Authors: Banda Frank , Sinkala Yona , Mataa Liywalli , Lebea Phiyani , Sikombe Tingiya , Kangwa Henry L. , Fana Elliot M. , Mokopasetso Mokganedi , Wadsworth Jemma , Knowles Nick J. , King Donald P. , Quan Melvyn
Published: November 2021

The livestock industry supports livelihood and nutritional security of at least 42% of people in the Southern African Development Community region. However, presence of animal diseases such as foot-and-mouth disease poses a major threat to the development of this industry. Samples collected from FMD outbreaks in Zambia during 2015–2020, comprising epithelial tissues samples (n = 47) and sera (n = 120), were analysed. FMD virus was serotyped in 26 samples, while 92 sera samples tested positive on NSP-ELISA. Phylogenetic analysis revealed notable changes in the epidemiology of FMD in Zambia, which included: (i) introduction of a novel FMDV SAT-3 (topotype II) causing FMD cases in cattle in Western Province; (ii) emergence of FMDV serotype O (topotype O/EA-2) in Central, Southern, Copperbelt, Western, Lusaka Provinces; and (iii) new outbreaks due to SAT -2 (topotypes I) in Eastern Zambia. Together, these data describe eight different epizootics that occurred in Zambia, four of which were outside the known FMD high-risk areas. This study highlights the complex epidemiology of FMD in Zambia, where the country represents an interface between East Africa (Pool 4) and Southern Africa (Pool 6). These changing viral dynamics have direct impacts on FMD vaccine selection in the SADC region.
Apoptosis Enhances the Replication of Human Coronavirus OC43
Authors: Maharjan Sony , Kang Mijeong , Kim Jinsoo , Kim Dongbum , Park Sangkyu , Kim Minyoung , Baek Kyeongbin , Lee Younghee , Kwon Hyung-Joo
Published: November 2021

Human coronavirus OC43 (HCoV-OC43) is one of the coronaviruses causing a mild common cold, but few studies have been made on this strain. Here, we identified the molecular mechanisms involved in HCoV-OC43-induced apoptosis and its implications for viral reproduction in Vero cells and MRC-5 cells. HCoV-OC43 infection induced apoptosis that was accompanied by cleavage of caspase-3 and PARP, degradation of cyclin D1, and cell cycle arrest at S and G2M phases. Dephosphorylation of STAT1 and STAT3, induced by HCoV-OC43 infection, was also associated with HCoV-OC43-mediated apoptosis. The pan-caspase inhibitor effectively prevented HCoV-OC43-induced apoptosis and reduced viral replication, suggesting that apoptosis contributes to viral replication. Collectively our results indicate that HCoV-OC43 induces caspase-dependent apoptosis to promote viral replication in Vero cells and MRC-5 cells.
Modulation of Expression of PVYNTN RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
Authors: Kozieł Edmund , Surowiecki Przemysław , Przewodowska Agnieszka , Bujarski Józef J. , Otulak-Kozieł Katarzyna
Published: November 2021

Potato virus Y (PVY) belongs to the genus Potyvirus and is considered to be one of the most harmful and important plant pathogens. Its RNA-dependent RNA polymerase (RdRp) is known as nuclear inclusion protein b (NIb). The recent findings show that the genome of PVY replicates in the cytoplasm of the plant cell by binding the virus replication complex to the membranous structures of different organelles. In some potyviruses, NIb has been found to be localized in the nucleus and associated with the endoplasmic reticulum membranes. Moreover, NIb has been shown to interact with other host proteins that are particularly involved in promoting the virus infection cycle, such as the heat shock proteins (HSPs). HSP70 is the most conserved among the five major HSP families that are known to affect the plant–pathogen interactions. Some plant viruses can induce the production of HSP70 during the development of infection. To understand the molecular mechanisms underlying the interactive response to PVYNTN (necrotic tuber necrosis strain of PVY), the present study focused on StHSC70-8 and PVYNTN-NIb gene expression via localization of HSC70 and NIb proteins during compatible (susceptible) and incompatible (hypersensitive) potato–PVYNTN interactions. Our results demonstrate that NIb and HSC70 are involved in the response to PVYNTN infections and probably cooperate at some stages of the virus infection cycle. Enhanced deposition of HSC70 proteins during the infection cycle was associated with the dynamic induction of PVYNTN-NIb gene expression and NIb localization during susceptible infections. In hypersensitive response (HR), a significant increase in HSC70 expression was observed up to 3 days post-inoculation (dpi) in the nucleus and chloroplasts. Thereafter, between 3 and 21 dpi, the deposition of NIb decreased, which can be attributed to a reduction in the levels of both virus accumulation and PVYNTN-NIb gene expression. Therefore, we postulate that increase in the expression of both StHSC70-8 and PVYNTN-NIb induces the PVY infection during susceptible infections. In contrast, during HRs, HSC70 cooperates with PVYNTN only at the early stages of interaction and mediates the defense response signaling pathway at the later stages of infection.
Estimation of Reduction in Influenza Vaccine Effectiveness Due to Egg-Adaptation Changes—Systematic Literature Review and Expert Consensus
Authors: Ortiz de Lejarazu-Leonardo Raul , Montomoli Emanuele , Wojcik Radek , Christopher Solomon , Mosnier Anne , Pariani Elena , Trilla Garcia Antoni , Fickenscher Helmut , Gärtner Barbara C. , Jandhyala Ravi , Zambon Maria , Moore Catherine
Published: November 2021

Background: Influenza vaccines are the main tool to prevent morbidity and mortality of the disease; however, egg adaptations associated with the choice of the manufacturing process may reduce their effectiveness. This study aimed to estimate the impact of egg adaptations and antigenic drift on the effectiveness of trivalent (TIV) and quadrivalent (QIV) influenza vaccines. Methods: Nine experts in influenza virology were recruited into a Delphi-style exercise. In the first round, the experts were asked to answer questions on the impact of antigenic drift and egg adaptations on vaccine match (VM) and influenza vaccine effectiveness (IVE). In the second round, the experts were presented with the data from a systematic literature review on the same subject and aggregated experts’ responses to round one questions. The experts were asked to review and confirm or amend their responses before the final summary statistics were calculated. Results: The experts estimated that, across Europe, the egg adaptations reduce, on average, VM to circulating viruses by 7–21% and reduce IVE by 4–16%. According to the experts, antigenic drift results in a similar impact on VM (8–24%) and IVE (5–20%). The highest reduction in IVE was estimated for the influenza virus A(H3N2) subtype for the under 65 age group. When asked about the frequency of the phenomena, the experts indicated that, on average, between the 2014 and 19 seasons, egg adaptation and antigenic drift were significant enough to impact IVE that occurred in two and three out of five seasons, respectively. They also agreed that this pattern is likely to reoccur in future seasons. Conclusions: Expert estimates suggest there is a potential for 9% on average (weighted average of “All strains” over three age groups adjusted by population size) and up to a 16% increase in IVE (against A(H3N2), the <65 age group) if egg adaptations that arise when employing the traditional egg-based manufacturing process are avoided.
Knowledge, Attitudes and Perception toward COVID-19 Vaccines among Adults in Jazan Province, Saudi Arabia
Authors: Alamer Edrous , Hakami Faisal , Hamdi Sulaiman , Alamer Afnan , Awaf Mohammed , Darraj Hussam , Abutalib Yumna , Madkhali Ebtisam , Alamer Rahaf , Bakri Nawaf , Qadri Marwa , Algaissi Abdullah , Alhazmi Abdulaziz
Published: November 2021

Background: Saudi Arabia is one of the countries that initiated early vaccination programs despite the global challenges concerning the availability of COVID-19 vaccines. Massive vaccination campaigns have been undertaken in the country; however, negative perception and hesitancy toward vaccines may exist which could reduce public response to vaccination. Further, studies evaluating the current perception and attitude toward COVID-19 vaccines are scarce. Thus, this study aims to assess the community attitudes and perceptions toward COVID-19 vaccines in Jazan Province, Saudi Arabia. Methods: A cross-sectional, retrospective study using an online questionnaire was conducted among the public in Jazan, the southern region of Saudi Arabia. General and demographic data were collected, and perception and attitude toward COVID-19 vaccines were evaluated. Results: Most participants in this study were female (67%) with a median age of 23 years. The majority held a bachelor’s degree, and they trusted the Saudi healthcare system. Our survey showed that 67% of the study participants had positive perceptions toward COVID-19 vaccines, a finding that is significantly associated with receiving the influenza vaccine in the past, the existence of trust on the current healthcare system and holding positive beliefs toward the effectiveness of the current COVID-19 vaccines in reducing the risk of infection, complication, and mortality. Conclusions: The proportion of the public in Jazan who believed in the COVID-19 vaccine effectiveness is not inferior from similar international reports. Thus, national awareness programs toward the effectiveness of the vaccine could be enhanced to accelerate vaccination coverage. Further, nationwide surveys are warranted to include larger populations from different communities to assess the overall perception toward COVID-19 vaccines in the whole country.
Recombinant BCG-Prime and DNA-Boost Immunization Confers Mice with Enhanced Protection against Mycobacterium kansasii
Authors: Komine-Aizawa Shihoko , Mizuno Satoru , Matsuo Kazuhiro , Namiki Takahiro , Hayakawa Satoshi , Honda Mitsuo
Published: November 2021

The incidence of infections with nontuberculous mycobacteria (NTM) has been increasing worldwide. The emergence of multidrug-resistant NTM is a serious clinical concern, and a vaccine for NTM has not yet been developed. We previously developed a new recombinant Bacillus Calmette–Guérin (rBCG) vaccine encoding the antigen 85B (Ag85B) protein of Mycobacterium kansasii—termed rBCG-Mkan85B—which was used together with a booster immunization with plasmid DNA expressing the same M. kansasii Ag85B gene (DNA-Mkan85B). We reported that rBCG-Mkan85B/DNA-Mkan85B prime–boost immunization elicited various NTM strain-specific CD4+ and CD8+ T cells and induced Mycobacterium tuberculosis-specific immunity. In this study, to investigate the protective effect against M. kansasii infection, we challenged mice vaccinated with a rBCG-Mkan85B or rBCG-Mkan85B/DNA-Mkan85B prime–boost strategy with virulent M. kansasii. Although BCG and rBCG-Mkan85B immunization each suppressed the growth of M. kansasii in the mouse lungs, the rBCG-Mkan85B/DNA-Mkan85B prime–boost vaccination reduced the bacterial burden more significantly. Moreover, the rBCG-Mkan85B/DNA-Mkan85B prime–boost vaccination induced antigen-specific CD4+ and CD8+ T cells. Our data suggest that rBCG-Mkan85B/DNA-Mkan85B prime–boost vaccination effectively enhances antigen-specific T cells. Our novel rBCG could be a potential alternative to clinical BCG for preventing various NTM infections.
An Established Th2-Oriented Response to an Alum-Adjuvanted SARS-CoV-2 Subunit Vaccine Is Not Reversible by Sequential Immunization with Nucleic Acid-Adjuvanted Th1-Oriented Subunit Vaccines
Authors: Cao Han , Yang Shengran , Wang Yunfei , Luan Ning , Yin Xingxiao , Lin Kangyang , Liu Cunbao
Published: November 2021

A recently reported parallel preclinical study between a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccine and an inactivated SARS-CoV-2 vaccine adjuvanted with alum showed pulmonary immunopathology typical of eosinophil accumulation in a mouse pneumonia model for the latter, which implied a potential role of cellular immunity in the difference in the protection rate between these two forms of vaccines. For those who have been vaccinated with alum-adjuvanted subunit or inactivated SARS-CoV-2 vaccines, whether the Th2 responses that have been established and the absence of induced cellular immunity could be changed is an open question. Using two heterologous boosts with Th1-oriented CpG ODN-adjuvanted S1-based SARS-CoV-2 subunit vaccines for mice that were primed with two doses of Th2-oriented alum-adjuvanted S1-based SARS-CoV-2 subunit vaccines, we demonstrated that established Th2 orientation could not be reversed to Th1 orientation and that no cellular immunity was induced, which should have been induced if the boosting vaccines were used as the prime vaccines. These results remind us that if widely administered alum-adjuvanted SARS-CoV-2 vaccines cannot overcome the challenge of coronavirus disease 2019 (COVID-19) and that if cellular immunity is important for the efficacy of SARS-CoV-2 vaccines in the future, the choice of more powerful heterologous boosting vaccine forms that can induce cellular immunity should be considered very carefully before application.
Development of an Inactivated Vaccine against SARS CoV-2
Authors: Pavel Shaikh Terkis Islam , Yetiskin Hazel , Uygut Muhammet Ali , Aslan Ahmet Furkan , Aydın Günsu , İnan Öznur , Kaplan Büşra , Ozdarendeli Aykut
Published: November 2021

The rapid spread of SARS-CoV-2 with its mutating strains has posed a global threat to safety during this COVID-19 pandemic. Thus far, there are 123 candidate vaccines in human clinical trials and more than 190 candidates in preclinical development worldwide as per the WHO on 1 October 2021. The various types of vaccines that are currently approved for emergency use include viral vectors (e.g., adenovirus, University of Oxford/AstraZeneca, Gamaleya Sputnik V, and Johnson & Johnson), mRNA (Moderna and Pfizer-BioNTech), and whole inactivated (Sinovac Biotech and Sinopharm) vaccines. Amidst the emerging cases and shortages of vaccines for global distribution, it is vital to develop a vaccine candidate that recapitulates the severe and fatal progression of COVID-19 and further helps to cope with the current outbreak. Hence, we present the preclinical immunogenicity, protective efficacy, and safety evaluation of a whole-virion inactivated SARS-CoV-2 vaccine candidate (ERUCoV-VAC) formulated in aluminium hydroxide, in three animal models, BALB/c mice, transgenic mice (K18-hACE2), and ferrets. The hCoV-19/Turkey/ERAGEM-001/2020 strain was used for the safety evaluation of ERUCoV-VAC. It was found that ERUCoV-VAC was highly immunogenic and elicited a strong immune response in BALB/c mice. The protective efficacy of the vaccine in K18-hACE2 showed that ERUCoV-VAC induced complete protection of the mice from a lethal SARS-CoV-2 challenge. Similar viral clearance rates with the safety evaluation of the vaccine in upper respiratory tracts were also positively appreciable in the ferret models. ERUCoV-VAC has been authorized by the Turkish Medicines and Medical Devices Agency and has now entered phase 3 clinical development (NCT04942405). The name of ERUCoV-VAC has been changed to TURKOVAC in the phase 3 clinical trial.
The Role of Pathology in the Diagnosis of Swine Respiratory Disease
Authors: Sarli Giuseppe , D’Annunzio Giulia , Gobbo Francesca , Benazzi Cinzia , Ostanello Fabio
Published: November 2021

The definition “porcine respiratory disease complex” (PRDC) is used to indicate the current approach for presenting respiratory pathology in modern pig farming. PRDC includes pneumonias with variable pictures, mixed with both aerogenous and hematogenous forms with variable etiology, often multimicrobial, and influenced by environmental and management factors. The notion that many etiological agents of swine respiratory pathology are ubiquitous in the airways is commonly understood; however, their isolation or identification is not always associable with the current pathology. In this complex context, lung lesions registered at slaughterhouse or during necropsy, and supplemented by histological investigations, must be considered as powerful tools for assigning a prominent role to etiologic agents. In recent years, the goal of colocalizing causative agents with the lesions they produce has been frequently applied, and valid examples in routine diagnostics are those that indicate pulmonary involvement during porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) infections.
Identification of Polymorphisms of the CSN2 Gene Encoding β-Casein in Greek Local Breeds of Cattle
Authors: Antonopoulos Dionysios , Vougiouklaki Despina , Laliotis George P. , Tsironi Theofania , Valasi Irene , Chatzilazarou Archodoula , Halvatsiotis Panagiotis , Houhoula Dimitra
Published: November 2021

This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein CSN2 gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated from 780 blood samples obtained from Greek Holstein cows, 86 from three local breeds of cattle, namely Brachyceros, Katerinis, and Sykias, and 14 from Greek buffalo. The desired region of exon 7 was amplified by PCR, resulting in 121 and 251 bp products in bovine and buffalo samples. The PCR product was digested with restriction fragment length polymorphism (RFLP) on agarose gels. The restriction enzymes DdeI and TaqI were used. All of the blood samples had the amplified size. The results showed that 74.4% of the Greek Holstein cows had the A2A2 β-casein genotype, the three native breads Vrachykeratiki had 57.7%, and the other two had 100% of the A2A2 β-casein. From the 14 Greek buffalo, 100% had the A2A2 β-casein.
Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals
Authors: Baliellas Jordi , Novell Elena , Enric-Tarancón Vicens , Vilalta Carles , Fraile Lorenzo
Published: November 2021

The detection capacity of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) in tongues from dead animals in breeding herds (stillborns and piglets dying during the lactating period) and nursery farms (naturally dead animals) for PRRSV surveillance was evaluated. The samples were selected if pairs of serum and tongues were available from 2018 to 2020. Serum (pools of five) and exudate from tongues (one bag) were analyzed by PRRSV RT-PCR. The agreement between the serum sample procedure versus tongues exudate was assessed using a concordance test (Kappa statistic) at batch level. A total of 32 submissions, corresponding to 14 farms, had PRRSV diagnostic information for serum and tongues exudate. The overall agreement of batch classification as positive or negative, based on RT-PCR PRRSV results, between serum and tongue exudate of the 32 pairs was 76.9%. Cohen’s Kappa was 0.55. The main discrepancy came from the presence of positive samples in tongues exudate and not in serum, suggesting that tongue exudate to monitor PRRSV seems to be more sensitive than serum. These results suggest that this sample procedure could be also used for PRRSV surveillance and monitoring.
Aura Mapping: Where Vision and Somatosensation Meet
Authors: Wilkinson Frances
Published: November 2021

While migraine auras are most frequently visual, somatosensory auras are also relatively common. Both are characterized by the spread of activation across a cortical region containing a spatial mapping of the sensory (retinal or skin) surface. When both aura types occur within a single migraine episode, they may offer an insight into the neural mechanism which underlies them. Could they both be initiated by a single neural event, or do the timing and laterality relationships between them demand multiple triggers? The observations reported here were carried out 25 years ago by a group of six individuals with migraine with aura. They timed, described and mapped their visual and somatosensory auras as they were in progress. Twenty-nine episode reports are summarized here. The temporal relationship between the onset of the two auras was quite variable within and across participants. Various forms of the cortical spreading depression hypothesis of migraine aura are evaluated in terms of whether they can account for the timing, pattern of symptom spread and laterality of the recorded auras.
Macrophytes: A Temporary Sink for Microplastics in Transitional Water Systems
Authors: Sfriso Andrea Augusto , Tomio Yari , Juhmani Abdul-Salam , Sfriso Adriano , Munari Cristina , Mistri Michele
Published: November 2021

Marine macrophytes are hypothesized to be a major temporary sink for microplastics. In this study, microplastic contamination was investigated in 15 macroalgal species and one seagrass from different sites in two lagoons of the northern Adriatic Sea: the Goro lagoon and the Venice lagoon. A high percentage (94%) of the macrophyte samples contained microplastics, ranging from 0.16 to 330 items g−1 fw, with the prevalent size in the range 30–90 µm and an average contamination per unit of fresh weight of 14 items g−1 fw. Microplastic contamination displayed a site-specific, rather than a species-specific, pattern of accumulation. In addition, exopolysaccharides (EPS) displayed a significant positive correlation with the microplastics ononcontamination on macrophytes acting as glue for the plastic particles available in the water column.
Global Sensitivity Analysis of Groundwater Related Dike Stability under Extreme Loading Conditions
Authors: van Woerkom Teun , van Beek Rens , Middelkoop Hans , Bierkens Marc F. P.
Published: November 2021

With up to 15% of the world’s population being protected by dikes from flooding, climate-change-induced river levels may dramatically increase the flood risk of these societies. Reliable assessments of dike stability will become increasingly important, but groundwater flow through dikes is often oversimplified due to limited understanding of the important process parameters. To improve the understanding of these parameters, we performed a global sensitivity analysis on a comprehensive hydro-stability model. The sensitivity analysis encompassed fifteen parameters related to geometry, drainage conditions and material properties. The following three sensitivity settings were selected to characterize model behavior: parameter prioritization, trend identification and interaction qualification. The first two showed that dike stability is mostly dependent on the dike slope, followed by the type of subsurface material. Interaction quantification indicated a very prominent interaction between the dike and subsurface material, as it influences both groundwater conditions and dike stability directly. Despite our relatively simple model setup, a database containing the results of the extensive Monte Carlo analysis succeeded in finding most of the unsafe sections identified by the official inspection results. This supports the applicability of our results and demonstrates that both geometry and subsurface parameters affect the groundwater conditions and dike stability.
The Multi-Scale Layering-Structure of Thermal Microscale Profiles
Authors: Folkard Andrew
Published: November 2021

Thermal microstructure profiling is an established technique for investigating turbulent mixing and stratification in lakes and oceans. However, it provides only quasi-instantaneous, 1-D snapshots. Other approaches to measuring these phenomena exist, but each has logistic and/or quality weaknesses. Hence, turbulent mixing and stratification processes remain greatly under-sampled. This paper contributes to addressing this problem by presenting a novel analysis of thermal microstructure profiles, focusing on their multi-scale stratification structure. Profiles taken in two small lakes using a Self-Contained Automated Micro-Profiler (SCAMP) were analysed. For each profile, buoyancy frequency (N), Thorpe scales (LT), and the coefficient of vertical turbulent diffusivity (KZ) were determined. To characterize the multi-scale stratification, profiles of d2T/dz2 at a spectrum of scales were calculated and the number of turning points in them counted. Plotting these counts against the scale gave pseudo-spectra, which were characterized by the index D of their power law regression lines. Scale-dependent correlations of D with N, LT and KZ were found, and suggest that this approach may be useful for providing alternative estimates of the efficiency of turbulent mixing and measures of longer-term averages of KZ than current methods provide. Testing these potential uses will require comparison of field measurements of D with time-integrated KZ values and numerical simulations.
Investigating Behavior of Six Methods for Sediment Transport Capacity Estimation of Spatial-Temporal Soil Erosion
Authors: Van Linh Nguyen , Le Xuan-Hien , Nguyen Giang V. , Yeon Minho , Jung Sungho , Lee Giha
Published: November 2021

Estimation of sediment transport capacity (STC) plays a crucial role in simulating soil erosion using any physics-based models. In this research, we aim to investigate the pros and cons of six popular STC methods (namely, Shear velocity, Kilinc-Richardson (KR), Effective stream power, Slope and unit discharge, Englund-Hansen (EH), and Unit stream power) for soil erosion/deposition simulation at watershed scales. An in-depth analysis was performed using the selected STC methods integrated into the Grid Surface Subsurface Hydrologic Analysis model for investigating the changes in morphology at spatial-temporal scales at the Cheoncheon watershed, South Korea, over three storm events. Conclusions were drawn as follows. (1) Due to the ability of the KR and EH methods to include an additional parameter (i.e., erodibility coefficient), they outperformed others by producing more accurate simulation results of sediment concentration predictions. The KR method also proved to be superior to the EH method when it showed a more suitable for sediment concentration simulations with a wide range of sediment size and forcing magnitude. (2) We further selected 2 STC methods among the 6 methods to deeply explore the spatial distribution of erosion/deposition. The overall results were more agreeable. For instance, the phenomenon of erosion mainly occurred upstream of watersheds with steep slopes and unbalanced initial sediment concentrations, whereas deposition typically appeared at locations with flat terrain (or along the mainstream). The EH method demonstrated the influence of topography (e.g., gradient slope) on accretionary erosion/deposition results more significantly than the KR method. The obtained results contribute a new understanding of rainfall-sediment-runoff processes and provide fundamental plans for soil conservation in watersheds.
Multivariate Statistical and Hydrochemical Analysis of Drinking Water Resources in Northern Cameroon Watersheds
Authors: Dammi Djimi Estelle Gaëlle , Abia Akebe Luther King , Belibi Belibi Placide Désiré , Takam Soh Patrice , Che Randy Nanga , Ghogomu Julius Numbonui , Ketcha Joseph Mbadcam
Published: November 2021

Watershed pollution by natural and anthropogenic activities remains a global challenge that requires careful and prompt attention. So, identifying possible pollution sources and studying the hydrochemistry of water resources would positively affect human health, especially in resource-limited communities and their economy. Water samples were collected during the rainy season in the North (R-NO) and Adamawa (R-AD) Region communities of Cameroon and assessed for physicochemical parameters using standard methods. The data were analysed using multivariate statistical and hydrochemical methods. Principal component analysis (PCA) retained seven and six principal components explaining 77.65% (R-NO) and 72.24% (R-AD) of the total variance, respectively. The drinking water sources assessed were highly, moderately, and lightly contaminated with turbidity, PO43−, Al3+, Fe2+, Mn2+, NH4+, NO3−, NO2−, and electrical conductivity (EC) from surface runoff and soil erosion sources. PCA and factor analysis (PCA/FA) revealed two main groups, distinguished by natural and anthropogenic sources, responsible for water quality variations. Hierarchical cluster analysis (HCA) grouped sampling sites into three clusters: low, moderate, and high pollution areas in the R-NO and unpolluted, low, and moderate pollution areas in the R-AD. The order of dominant cations was Mg2+ > Ca2+ > K+ and HCO3− > Cl− > SO42− for anions. Based on Piper diagram classification, watersheds studied were predominated by the Mg-Ca-HCO3 water type in 85% (R-NO) and 79% (R-AD) of water samples. The chemical composition of shallow and deep water was dominantly controlled by the dissolution of silicates and carbonate, reverse ion exchange, and precipitation of calcite. These results reveal that diffuse pollution predominantly impacted the study sites during the rainy season, and this should be the focus of policymakers when planning and implementing measures to protect drinking water sources, human health, and reduce water treatment costs.
Effects of Randomly Fired Underwater Currents as an Occupational Enrichment Program in Rainbow Trout (Oncorhynchus mykiss)
Authors: Villarroel Morris , Miranda-de la Lama Genaro C. , Bermejo-Poza Rubén , Pérez Concepción , González-de Chávarri Elisabet , Torrent Fernando , De la Fuente Jesús
Published: November 2021

Occupational enrichment (OE) is directed at introducing variations in the tank water so that fish can exercise as they do in the wild. Two trials were carried out to test the effects of randomly fired underwater currents (RFC) on rainbow trout (Oncorhynchus mykiss) maintained in tanks in a recirculation system, using 1226 trout distributed in two independent trials. In Trial 1, fingerling trout (n = 6 tanks, n = 40 fish per tank) were classified into two groups based on low (13%) or high (30%) coefficient of variation in live weight (CV), and exposed to RFC or no currents (controls). In Trial 2, adult trout (n = 12 tanks, n = 20 fish per tank) were either exposed to RFC or to a constant current (controls) from two submerged pumps. Both trials lasted four weeks. No significant differences in growth were observed between treatments in either trial. In Trial 1, RFC fish maintained a similar CV throughout the trial, while CV decreased in controls. Also, in Trial 1, plasma cortisol levels were higher and creatine phosphokinase (CPK) levels lower in tanks with a low initial CV. In Trial 2, the CV was lower in RFC trout, where cortisol levels were also significantly lower and triglycerides significantly higher. The results suggest that OE using RFC can have positive effects by helping to reduce stress levels, and provides fish with biologically meaningful environmental enrichment related to the natural history of the species.
Evaluation of the Impact of Ecological Water Supplement on Groundwater Restoration Based on Numerical Simulation: A Case Study in the Section of Yongding River, Beijing Plain
Authors: Ji Zijian , Cui Yali , Zhang Shouquan , Chao Wan , Shao Jingli
Published: November 2021

Ecological water supplement relies on river channels to introduce surface water, to make a reasonable supplement of groundwater, to repair the regional groundwater environment and urban river ecosystem. Evaluating the degree of groundwater restoration after ecological water supplement (by taking appropriate measures) is a critical problem that needs to be solved. Thus, based on the Yongding River ecological water supplement in 2019 and 2020, we analyzed the groundwater monitoring situates in the ecological water supplement region. We established an unstructured groundwater flow numerical model in the study area through the quadtree grids. The model was calibrated with the measured water level. The simulated results could accurately reflect the real groundwater dynamic characteristics, and it showed that the water level rise was concentrated in the 3–6 km range of the Yongding River after the ecological water supplement. In 2019, the calculated ecological water infiltration amount was 101.28 × 106 m3, the affected area was 265.19 km2, and the average groundwater level rise in the affected area was 2.10 m. In 2020, the calculated ecological water infiltration amount was 102.64 × 106 m3, the affected area was 506.88 km2, and the average groundwater level rise in the affected area was 1.25 m. While the ecological water supplement had a positive impact on groundwater level restoration, the groundwater level around the typical buildings within the study area, including Beijing West Railway Station and Beijing Daxing International Airport, would not be significantly affected.
Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of Parachlorella Kessleri TY
Authors: Gao Yifan , Ji Li , Feng Jia , Lv Junping , Xie Shulian
Published: November 2021

The combustion explosion of ordinary diesel is a global environmental problem. Green microalgae, which do not cause eutrophication, are a raw material that can be used to clean biodiesel. To increase lipid productivity, this study used a nitrogen deficient & mixotrophic (+Glucose) culture of lipid-producing microalgae Parachlorella kessleri TY from Shanxi Province, China. To examine the growth of P. kessleri TY, we measured dry weight, chlorophyll content, and chlorophyll fluorescence intensity under different culture conditions, in addition to the contents of neutral lipids, total lipids, and fatty acids, to examine its lipid accumulation ability. Cells were cultured in autotrophic, nitrogen deficient, mixotrophic (+Glucose), and nitrogen deficient & mixotrophic (+Glucose) conditions for 7 days. We found the growth of P. kessleri TY under nitrogen deficient & mixotrophic conditions was higher than that under the autotrophic and nitrogen deficient conditions, but lower than that under the mixotrophic (+Glucose) conditions. However, its lipid accumulation ability was significantly higher than that of control cultures. In conclusion, P. kessleri TY cultured under nitrogen-deficient and mixotrophic (+Glucose) conditions has significant lipid production capacity. Our results provide a theoretical basis for the use of microalgae as a raw material in the production of biodiesel, and promote the application of P. kessleri TY in large-scale production.