bluuebunny/crossref_metadata_2025 huggingface dataset:
Rise, Fall, and Recovery of Blockchains in the Maritime Technology Space
Authors: Munim Ziaul Haque , Duru Okan , Hirata Enna
Published: March 2021

Blockchain technology, since its introduction, has been expected to be implemented in many areas. Cryptocurrency is one unique example that established a functioning application. On the other hand, blockchain technology is not immune to various challenges related to the nature of itself, privacy management, and antitrust laws, among others. This study lays out the nature of blockchain and applications in the maritime industry, while highlighting the bottlenecks. Potential resolutions and anticipated developments are proposed. To do this, we adopt a systematic approach and present an overview of blockchain in maritime literature. In addition, the fundamental problems with blockchain are investigated, beginning from their essentials to the pain points that are claimed to need improvement. For establishing a legitimate and practically meaningful blockchain platform, stakeholders need to achieve pluralism (consensus validation), privacy, and security of the system.
Investigations on the Scuffing and Wear Characteristic Performance of an Oil Free Water-Based Lubricant for Gear Applications
Authors: Sagraloff Nadine , Winkler Karl Jakob , Tobie Thomas , Stahl Karsten , Folland Christopher , Asam Tobias
Published: March 2021

In most gear drive applications mineral or synthetic oils are used as lubricants, which are made of fossil raw materials and are non-biodegradable. In applications located in critical environmental areas such as boats or harbors, eco-friendly lubricants are needed. As a result, a gear transmission fluid based on water is currently being developed in a research project supported by the Bayrische Forschungsstiftung (Bavarian Research Foundation). Results of former research showed that in general it is possible to use water-based lubricants in gear drives under certain operating conditions. Since water has a low viscosity compared to conventional used lubricants, plant extracts are added to generate higher viscosities. In order to avoid tribological influenced damages such as sliding wear and scuffing on the surface of gear flanks, adequate additives are needed. Different combinations of plant extracts and additives were investigated using the scuffing test A/8.3/RT according to DIN ISO 14635-1. The results show a surprisingly high load carrying capacity regarding scuffing. Additionally, two wear tests based on DGMK 377-01 were conducted with one sample fluid. A high risk of sliding wear was detected. Additionally, MTM and SRV measurements were conducted with different polymers to optimize the lubricant. The results of the wear tests help to define operating conditions for a future lubricant based on water and plant extracts. This paper aims to share the results of the performed experimental investigations and discusses the challenges regarding the development of such new lubricants.
3D Finite Element Model on Drilling of CFRP with Numerical Optimization and Experimental Validation
Authors: Hale Patrick , Ng Eu-Gene
Published: March 2021

When drilling Carbon Fibre-Reinforced Plastic (CFRP) materials, achieving acceptable hole quality is challenging while balancing productivity and tool wear. Numerical models are important tools for the optimization of drilling CFRP materials in terms of material removal rate and hole quality. In this research, a macro-Finite Element (FE) model was developed to accurately predict the effect of drill tip geometry on hole entry and exit quality. The macro-mechanical material model was developed treating the Fiber-Reinforced Plastic (FRP) as an Equivalent Homogeneous Material (EHM). To reduce computational time, a numerical analysis was performed to investigate the influence of mass scaling, bulk viscosity, friction, strain rate strengthening, and cohesive surface modelling. A consideration must be made to minimize the dynamic effects in the FE prediction. The experimental work was carried out to investigate the effect of drill tip geometry on drilling forces and hole quality and to validate the FE results. The geometry of the drills used were either double-point angle or a “candle-stick” profile. The 3D drilling model accurately predicts the thrust force and hole quality generated by the two different drills. The results highlight the improvement in predicted results with the inclusion of cohesive surface modelling. The force signature profiles between the simulated and experimental results were similar. Furthermore, the difference between the predicted thrust force and those measured were less than 9%. When drilling with a double-angle drill tip, the inter-ply damage was reduced. This trend was observed in FE prediction.
New Preconditioned Iteration Method Solving the Special Linear System from the PDE-Constrained Optimal Control Problem
Authors: Li Yan-Ran , Shao Xin-Hui , Li Shi-Yu
Published: March 2021

In many fields of science and engineering, partial differential equation (PDE) constrained optimal control problems are widely used. We mainly solve the optimization problem constrained by the time-periodic eddy current equation in this paper. We propose the three-block splitting (TBS) iterative method and proved that it is unconditionally convergent. At the same time, the corresponding TBS preconditioner is derived from the TBS iteration method, and we studied the spectral properties of the preconditioned matrix. Finally, numerical examples in two-dimensions is applied to demonstrate the advantages of the TBS iterative method and TBS preconditioner with the Krylov subspace method.
Induction of Apoptotic Cell Death in Human Leukemia U937 Cells by C18 Hydroxy Unsaturated Fatty Acid Isolated from Red Alga Tricleocarpa jejuensis
Authors: Zha Shijiao , Ueno Mikinori , Liang Yan , Okada Seiji , Oda Tatsuya , Ishibashi Fumito
Published: March 2021

Our previous studies have found that (±)-(E)-12-hydroxyoctadec-10-enoic acid (HOEA) isolated from the red alga Tricleocarpa jejuensis showed cytotoxic effects on various living organisms including harmful microalgae, Gram-positive bacteria, and mammalian tumor cells. Since natural products with apoptosis-inducing ability can be promising anti-cancer agents, in this study, we investigated the cytotoxic mechanism of HOEA on U937 cells focusing on apoptosis induction. HOEA showed much stronger cytotoxic and cytolytic effects on U937 cells than elaidic acid, which has similar structure but no 12-hydroxy group, suggesting that hydroxy group is important for the cytotoxicity of HOEA. HOEA induced apoptotic nuclear morphological changes, DNA fragmentation, and decrease in mitochondrial membrane potential. Furthermore, time-dependent increase in annexin V+/PI+ cell population in HOEA-treated U937 cells was detected. Among the apoptosis-related reagents, caspase-family inhibitor almost completely inhibited HOEA-induced DNA fragmentation. In the analyses using specific caspase-substrates, extremely high cleavage activity toward caspase-3/7/8 substrate was observed in HOEA-treated U937 cells, and weak activities of caspase-1 and -3 were detected. Analyses using specific caspase inhibitors suggested that caspase-3 and caspase-8 might be predominantly responsible for the cleavage activity. Activation of these caspases were also confirmed by western blotting in which significant levels of cleaved forms of caspase 3, caspase 8, and PARP were detected in HOEA-treated U937 cells. Our results suggest that HOEA is capable of inducing apoptosis in U937 cells in which caspase-3 and caspase-8 might play important roles. Since the cytotoxic effect of HOEA is not strictly specific to tumor cells, development of appropriate drug delivery system for selective tumor targeting is necessary for the clinical applications to reduce the possible side effects.
Complementary Analytical Platforms of NMR Spectroscopy and LCMS Analysis in the Metabolite Profiling of Isochrysis galbana
Authors: Bustamam Muhammad Safwan Ahamad , Pantami Hamza Ahmed , Azizan Awanis , Shaari Khozirah , Min Chong Chou , Abas Faridah , Nagao Norio , Maulidiani Maulidiani , Banerjee Sanjoy , Sulaiman Fadzil , Ismail Intan Safinar
Published: March 2021

This study was designed to profile the metabolites of Isochrysis galbana, an indigenous and less explored microalgae species. 1H Nuclear Magnetic Resonance (NMR) spectroscopy and Liquid Chromatography-Mass Spectrometry (LCMS) were used to establish the metabolite profiles of five different extracts of this microalga, which are hexane (Hex), ethyl acetate (EtOAc), absolute ethanol (EtOH), EtOH:water 1:1 (AqE), and 100% water (Aq). Partial least square discriminant analysis (PLS–DA) of the generated profiles revealed that EtOAc and Aq extracts contain a diverse range of metabolites as compared to the other extracts with a total of twenty-one metabolites, comprising carotenoids, polyunsaturated fatty acids, and amino acids, that were putatively identified from the NMR spectra. Meanwhile, thirty-two metabolites were successfully annotated from the LCMS/MS data, ten of which (palmitic acid, oleic acid, α-linolenic acid, arachidic acid, cholesterol, DHA, DPA, fucoxanthin, astaxanthin, and pheophytin) were similar to those present in the NMR profile. Another eleven glycerophospholipids were discovered using MS/MS-based molecular network (MN) platform. The results of this study, besides providing a better understanding of I.galbana’s chemical make-up, will be of importance in exploring this species potential as a feed ingredient in the aquaculture industry.
Laboratory and Field Evaluation of the Phytotoxic Activity of Sapindus mukorossi Gaertn Pulp Extract and Identification of a Phytotoxic Substance
Authors: Dai Ziyang , Wang Jin , Ma Xiaojiang , Sun Jia , Tang Feng
Published: March 2021

Interest in finding plant-based herbicides to supplement synthesized herbicides is increasing. Although the extract of Sapindus mukorossi Gaertn has been reported to have herbicidal activity, little is known about phytotoxic substances and their efficacy of weed control in the field. To identify phytotoxic substances, the bioassay-guided fractionation by column chromatography and high-speed counter-current chromatography (HSCCC) was carried out. The phytotoxic activity assay, performed by the agar medium method, showed that the 70% ethanol fraction exhibited strong root growth inhibition against Trifolium pratense with an 50% inhibitory concentration (IC50) value of 35.13 mg/L. An active compound was isolated from the 70% ethanol fraction and identified as hederagenin 3-o-β-D-xylopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→2)-α-l-arabinopyranoside (Compound A). Compound A had an IC50 value of 16.64 mg/L. Finally, a new formulation was prepared based on the 70% ethanol fraction, which exhibited good efficacy against broadleaf weeds in a carrot field. The fresh weight control efficacy was 78.7% by 45 days after treatment at the dose of 1500 g a. i./ha. Hence, the extract of S. mukorossi pulp could be a promising supplement to the synthesized herbicides. Furthermore, compound A from S. mukorossi may be responsible for its phytotoxic activity.
Synthesis of Bis(Carboranyl)amides 1,1′-μ-(CH2NH(O)C(CH2)n-1,2-C2B10H11)2 (n = 0, 1) and Attempt of Synthesis of Gadolinium Bis(Dicarbollide)
Authors: Asawa Yasunobu , Arsent’eva Aleksandra V. , Anufriev Sergey A. , Anisimov Alexei A. , Suponitsky Kyrill Yu. , Filippov Oleg A. , Nakamura Hiroyuki , Sivaev Igor B.
Published: March 2021

Bis(carboranyl)amides 1,1′-μ-(CH2NH(O)C(CH2)n-1,2-C2B10H11)2 (n = 0, 1) were prepared by the reactions of the corresponding carboranyl acyl chlorides with ethylenediamine. Crystal molecular structure of 1,1′-μ-(CH2NH(O)C-1,2-C2B10H11)2 was determined by single crystal X-ray diffraction. Treatment of bis(carboranyl)amides 1,1′-μ-(CH2NH(O)C(CH2)n-1,2-C2B10H11)2 with ammonium or cesium fluoride results in partial deboronation of the ortho-carborane cages to the nido-carborane ones with formation of [7,7′(8′)-μ-(CH2NH(O)C(CH2)n-7,8-C2B9H11)2]2−. The attempted reaction of [7,7′(8′)-μ-(CH2NH(O)CCH2-7,8-C2B9H11)2]2− with GdCl3 in 1,2-dimethoxy- ethane did not give the expected metallacarborane. The stability of different conformations of Gd-containing metallacarboranes has been estimated by quantum-chemical calculations using [3,3-μ-DME-3,3′-Gd(1,2-C2B9H11)2]− as a model. It was found that in the most stable conformation the CH groups of the dicarbollide ligands are in anti,anti-orientation with respect to the DME ligand, while any rotation of the dicarbollide ligand reduces the stability of the system. This makes it possible to rationalize the design of carborane ligands for the synthesis of gadolinium metallacarboranes on their base.
Pathogenic Roles of S100A8 and S100A9 Proteins in Acute Myeloid and Lymphoid Leukemia: Clinical and Therapeutic Impacts
Authors: Mondet Julie , Chevalier Simon , Mossuz Pascal
Published: March 2021

Deregulations of the expression of the S100A8 and S100A9 genes and/or proteins, as well as changes in their plasma levels or their levels of secretion in the bone marrow microenvironment, are frequently observed in acute myeloblastic leukemias (AML) and acute lymphoblastic leukemias (ALL). These deregulations impact the prognosis of patients through various mechanisms of cellular or extracellular regulation of the viability of leukemic cells. In particular, S100A8 and S100A9 in monomeric, homodimeric, or heterodimeric forms are able to modulate the survival and the sensitivity to chemotherapy of leukemic clones through their action on the regulation of intracellular calcium, on oxidative stress, on the activation of apoptosis, and thanks to their implications, on cell death regulation by autophagy and pyroptosis. Moreover, biologic effects of S100A8/9 via both TLR4 and RAGE on hematopoietic stem cells contribute to the selection and expansion of leukemic clones by excretion of proinflammatory cytokines and/or immune regulation. Hence, the therapeutic targeting of S100A8 and S100A9 appears to be a promising way to improve treatment efficiency in acute leukemias.
Behaviors of Perfluorocarbon Emulsion during Dissolution of Oxide Layers
Authors: Chang Naon , Won Huijun , Jung Chonghun , Kim Seonbyeong , Eun Heechul , Kim Yongsoo
Published: March 2021

This study investigates the dissolution behavior of oxide layers containing radionuclides using perfluorocarbon (PFC) emulsion as a reusable medium. Chemicals such as PFC, anionic surfactant, and H2SO4 are used for preparing the PFC emulsion, and emulsified using an ultrasonication process. The FTIR results show O–H stretching that is formed by the interaction of the carboxyl group of the anionic surfactant with the hydroxyl group of water containing H2SO4, and find that the H2SO4 can be homogeneously dispersed in the PFC–anionic surfactant–H2SO4 emulsion. The dissolution test of the simulated Cr2O3 specimen is conducted using PFC emulsion containing KMnO4. Through the weight losses of specimens and Scanning Electron Microscope-Energy Dispersive X-ray Spectrometer (SEM-EDS) analysis, it is confirmed that the Cr2O3 layer on the SUS304 specimen is easily dissolved using PFC emulsion. During the dissolution of the Cr2O3, it is observed that the dispersed H2SO4–KMnO4 became unstable and separated from PFC emulsion. Based on these results, the behavior of the PFC emulsion during the dissolution of the oxide layer is explained.
The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats
Authors: Iova Gilda M. , Calniceanu Horia , Popa Adelina , Szuhanek Camelia A. , Marcu Olivia , Ciavoi Gabriela , Scrobota Ioana
Published: March 2021

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.
Intracellular Responses Triggered by Cold Atmospheric Plasma and Plasma-Activated Media in Cancer Cells
Authors: Motaln Helena , Recek Nina , Rogelj Boris
Published: March 2021

Cold atmospheric plasma (CAP), an ionized gas operating at room temperature, has been increasingly studied with respect to its potential use in medicine, where its beneficial effects on tumor reduction in oncology have been demonstrated. This review discusses the cellular changes appearing in cell membranes, cytoplasm, various organelles, and DNA content upon cells’ direct or indirect exposure to CAP or CAP-activated media/solutions (PAM), respectively. In addition, the CAP/PAM impact on the main cellular processes of proliferation, migration, protein degradation and various forms of cell death is addressed, especially in light of CAP use in the oncology field of plasma medicine.
Folate Levels in Patients Hospitalized with Coronavirus Disease 2019
Authors: Meisel Eshcar , Efros Orly , Bleier Jonathan , Beit Halevi Tal , Segal Gad , Rahav Galia , Leibowitz Avshalom , Grossman Ehud
Published: March 2021

We aimed to investigate the prevalence of decreased folate levels in patients hospitalized with Coronavirus Disease 2019 (COVID-19) and evaluate their outcome and the prognostic signifi-cance associated with its different levels. In this retrospective cohort study, data were obtained from the electronic medical records at the Sheba Medical Center. Folic acid levels were available in 333 out of 1020 consecutive patients diagnosed with COVID-19 infection hospitalized from January 2020 to November 2020. Thirty-eight (11.4%) of the 333 patients comprising the present study population had low folate levels. No significant difference was found in the incidence of acute kidney injury, hypoxemia, invasive ventilation, length of hospital stay, and mortality be-tween patients with decreased and normal-range folate levels. When sub-dividing the study population according to quartiles of folate levels, similar findings were observed. In conclusion, decreased serum folate levels are common among hospitalized patients with COVID-19, but there was no association between serum folate levels and clinical outcomes. Due to the important role of folate in cell metabolism and the potential pathologic impact when deficient, a follow-up of folate levels or possible supplementation should be encouraged in hospitalized COVID-19 patients. Fur-ther studies are required to assess the prevalence and consequences of folate deficiency in COVID-19 patients.
Serum Concentration of Antibodies to Mumps, but Not Measles, Rubella, or Varicella, Is Associated with Intake of Dietary Fiber in the NHANES, 1999–2004
Authors: Van Landingham Cynthia B. , Keast Debra R. , Longnecker Matthew P.
Published: March 2021

Treatment with prebiotics, a type of dietary fiber, was recently shown to increase antibody concentrations following influenza vaccination in a meta-analysis of clinical trials. In observational epidemiologic studies it is not possible to estimate intake of prebiotics, but quantifying intake of dietary fiber is routine. Our objective was to investigate the potential effect of dietary fiber on immunogenicity. We examined serum antibody concentrations (Measles, Mumps, Rubella, and Varicella) in relation to dietary fiber in more than 12,000 subjects in the U.S. National Health and Nutrition Examination Survey (NHANES) for the period 1999–2004. Data from one (1999–2002) or two (2003–2004) dietary recalls were used to calculate fiber intake. For Mumps the adjusted percentage difference in antibody concentration per interquartile range intake in energy-adjusted dietary fiber was 6.34% (95% confidence interval, 3.10, 9.68). Fiber from grain-based foods was more positively associated than fiber from other fiber-containing food groups. The association was slightly larger among subgroups with higher fiber intake, greater interquartile range in fiber intake, and less measurement error. Furthermore, based on the reliability of the diet recalls in 2003–2004, we calculated that the percentage difference per interquartile increment was substantially attenuated by measurement error. Dietary fiber may have a favorable influence on the immunogenicity of some vaccines or natural infections.
Caffeine-Induced Effects on Human Skeletal Muscle Contraction Time and Maximal Displacement Measured by Tensiomyography
Authors: Domaszewski Przemysław , Pakosz Paweł , Konieczny Mariusz , Bączkowicz Dawid , Sadowska-Krępa Ewa
Published: March 2021

Studies on muscle activation time in sport after caffeine supplementation confirmed the effectiveness of caffeine. The novel approach was to determine whether a dose of 9 mg/kg/ body mass (b.m.) of caffeine affects the changes of contraction time and the displacement of electrically stimulated muscle (gastrocnemius medialis) in professional athletes who regularly consume products rich in caffeine and do not comply with the caffeine discontinuation period requirements. The study included 40 professional male handball players (age = 23.13 ± 3.51, b.m. = 93.51 ± 15.70 kg, height 191 ± 7.72, BMI = 25.89 ± 3.10). The analysis showed that in the experimental group the values of examined parameters were significantly reduced (p ≤ 0.001) (contraction time: before = 20.60 ± 2.58 ms/ after = 18.43 ± 3.05 ms; maximal displacement: before = 2.32 ± 0.80 mm/after = 1.69 ± 0.51 mm). No significant changes were found in the placebo group. The main achievement of this research was to demonstrate that caffeine at a dose of 9 mg/kg in professional athletes who regularly consume products rich in caffeine has a direct positive effect on the mechanical activity of skeletal muscle stimulated by an electric pulse.
Vegan Diet Health Benefits in Metabolic Syndrome
Authors: Marrone Giulia , Guerriero Cristina , Palazzetti Daniela , Lido Paolo , Marolla Alessandro , Di Daniele Francesca , Noce Annalisa
Published: March 2021

Plant-based diets (PBDs) are increasingly consumed by the Italian population and around the world. In particular, among PBDs, the vegan diet is a food pattern characterized by the exclusion of all animal-origin foods. What drives people to adopt this model are mainly ethical, health and environmental reasons. A vegan diet, if well-balanced and varied, can help in achieving and maintaining an optimal state of health. However, this nutritional approach, if not well-balanced, can cause deficiencies in proteins, ω-3 fatty acids, iron, vitamin D and calcium, zinc, iodine and, above all, vitamin B12. Oral food supplements especially fortified foods are recommended in these cases to restore the nutritional deficiencies. A vegan diet generally reduces the risk of developing chronic non-communicable degenerative diseases, such as metabolic syndrome (MetS) and, in addition, requires fewer natural resources for food production than an omnivorous diet. The aim of this review is to analyze the possible impact of the vegan diet on MetS onset and its treatment.
Association between Single Nucleotide Polymorphisms and Weight Reduction in Behavioural Interventions—A Pooled Analysis
Authors: Holzapfel Christina , Sag Sabine , Graf-Schindler Johanna , Fischer Marcus , Drabsch Theresa , Illig Thomas , Grallert Harald , Stecher Lynne , Strack Christina , Caterson Ian D. , Jebb Susan A. , Hauner Hans , Baessler Andrea
Published: March 2021

Knowledge of the association between single nucleotide polymorphisms (SNPs) and weight loss is limited. The aim was to analyse whether selected obesity-associated SNPs within the fat mass and obesity-associated (FTO), transmembrane protein 18 (TMEM18), melanocortin-4 receptor (MC4R), SEC16 homolog B (SEC16B), and brain-derived neurotrophic factor (BDNF) gene are associated with anthropometric changes during behavioural intervention for weight loss. genetic and anthropometric data from 576 individuals with overweight and obesity from four lifestyle interventions were obtained. A genetic predisposition score (GPS) was calculated. Our results show that study participants had a mean age of 48.2 ± 12.6 years and a mean baseline body mass index of 33.9 ± 6.4 kg/m2. Mean weight reduction after 12 months was −7.7 ± 10.9 kg. After 12 months of intervention, the MC4R SNPs rs571312 and rs17782313 were significantly associated with a greater decrease in body weight and BMI (p = 0.012, p = 0.011, respectively). The investigated SNPs within the other four genetic loci showed no statistically significant association with changes in anthropometric parameters. The GPS showed no statistically significant association with weight reduction. In conclusion there was no consistent evidence for statistically significant associations of SNPs with anthropometric changes during a behavioural intervention. It seems that other factors play a more significant in weight management than the investigated SNPs.
‘An Apple a Day’?: Psychiatrists, Psychologists and Psychotherapists Report Poor Literacy for Nutritional Medicine: International Survey Spanning 52 Countries
Authors: Mörkl Sabrina , Stell Linda , Buhai Diana V. , Schweinzer Melanie , Wagner-Skacel Jolana , Vajda Christian , Lackner Sonja , Bengesser Susanne A. , Lahousen Theresa , Painold Annamaria , Oberascher Andreas , Tatschl Josef M. , Fellinger Matthäus , Müller-Stierlin Annabel , Serban Ana C. , Ben-Sheetrit Joseph , Vejnovic Ana-Marija , Butler Mary I. , Balanzá-Martínez Vicent , Zaja Nikola , Rus-Prelog Polona , Strumila Robertas , Teasdale Scott B. , Reininghaus Eva Z. , Holasek Sandra J.
Published: March 2021

Nutritional interventions have beneficial effects on certain psychiatric disorder symptomatology and common physical health comorbidities. However, studies evaluating nutritional literacy in mental health professionals (MHP) are scarce. This study aimed to assess the across 52 countries. Surveys were distributed via colleagues and professional societies. Data were collected regarding self-reported general nutrition knowledge, nutrition education, learning opportunities, and the tendency to recommend food supplements or prescribe specific diets in clinical practice. In total, 1056 subjects participated in the study: 354 psychiatrists, 511 psychologists, 44 psychotherapists, and 147 MHPs in-training. All participants believed the diet quality of individuals with mental disorders was poorer compared to the general population (p < 0.001). The majority of the psychiatrists (74.2%) and psychologists (66.3%) reported having no training in nutrition. Nevertheless, many of them used nutrition approaches, with 58.6% recommending supplements and 43.8% recommending specific diet strategies to their patients. Only 0.8% of participants rated their education regarding nutrition as ‘very good.’ Almost all (92.9%) stated they would like to expand their knowledge regarding ‘Nutritional Psychiatry.’ There is an urgent need to integrate nutrition education into MHP training, ideally in collaboration with nutrition experts to achieve best practice care.
Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation
Authors: Chataigner Mathilde , Martin Marie , Lucas Céline , Pallet Veronique , Layé Sophie , Mehaignerie Alexis , Bouvret Elodie , Dinel Anne-Laure , Joffre Corinne
Published: March 2021

Neuroinflammation constitutes a normal part of the brain immune response orchestrated by microglial cells. However, a sustained and uncontrolled production of proinflammatory factors together with microglial activation contribute to the onset of a chronic low-grade inflammation, leading to neuronal damage and cognitive as well as behavioral impairments. Hence, limiting brain inflammatory response and improving the resolution of inflammation could be particularly of interest to prevent these alterations. Dietary n-3 long chain polyunsaturated fatty acids (LC-PUFAs) and low molecular weight peptides are good candidates because of their immunomodulatory and proresolutive properties. These compounds are present in a fish hydrolysate derived from marine-derived byproducts. In this study, we compared the effect of an 18-day supplementation with this fish hydrolysate to a supplementation with docosahexaenoic acid (DHA) on lipopolysaccharide (LPS)-induced inflammation in mice. In response to peripherally injected LPS, the fish hydrolysate supplementation decreased the hippocampal mRNA expression of the proinflammatory cytokines IL-6 (p < 0.001), IL-1β (p = 0.0008) and TNF-α (p < 0.0001), whereas the DHA supplementation reduced only the expression of IL-6 (p = 0.004). This decline in proinflammatory cytokine expressions was associated with an increase in the protein expression of IκB (p = 0.014 and p = 0.0054 as compared to the DHA supplementation and control groups, respectively) and to a modulation of microglial activation markers in the hippocampus. The beneficial effects of the fish hydrolysate could be due in part to the switch of the hippocampal oxylipin profile towards a more anti-inflammatory profile as compared to the DHA supplementation. Thus, the valorization of fish byproducts seems very attractive to prevent and counteract neuroinflammation.
Molecular Characterization of Carbapenem Resistant Klebsiella pneumoniae in Malaysia Hospital
Authors: Lau Min Yi , Teng Fui Enn , Chua Kek Heng , Ponnampalavanar Sasheela , Chong Chun Wie , Abdul Jabar Kartini , Teh Cindy Shuan Ju
Published: March 2021

The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) is a great concern, as carbapenems are the last-line therapy for multidrug-resistant Gram-negative bacteria infections. This study aims to report the epidemiology of CRKP in a teaching hospital in Malaysia based on the molecular genotypic and clinical characteristics of the isolates. Sixty-three CRKP strains were isolated from a tertiary teaching hospital from January 2016 until August 2017. Carbapenemase genes were detected in 55 isolates, with blaOXA-48 (63.5%) as the predominant carbapenemase gene, followed by blaNDM (36.5%). At least one porin loss was detected in nine isolates. Overall, 63 isolates were divided into 30 clusters at similarity of 80% with PFGE analysis. Statistical analysis showed that in-hospital mortality was significantly associated with the usage of central venous catheter, infection or colonization by CRKP, particularly NDM-producers. In comparison, survival analysis using Cox proportional hazards regression identified a higher hazard ratio for patients with a stoma and patients treated with imipenem but a lower hazard ratio for patients with NDM-producing CRKP. OXA-48 carbapenemase gene was the predominant carbapenemase gene in this study. As CRKP infection could lead to a high rate of in-hospital mortality, early detection of the isolates was important to reduce their dissemination.
Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations
Authors: Menu Estelle , Davoust Bernard , Mediannikov Oleg , Akiana Jean , Mulot Baptiste , Diatta Georges , Levasseur Anthony , Ranque Stéphane , Raoult Didier , Bittar Fadi
Published: March 2021

Non-human primate populations act as potential reservoirs for human pathogens, including viruses, bacteria and parasites, which can lead to zoonotic infections. Furthermore, intestinal microorganisms may be pathogenic organisms to both non-human primates and humans. It is, therefore, essential to study the prevalence of these infectious agents in captive and wild non-human primates. This study aimed at showing the prevalence of the most frequently encountered human enteric protozoa in non-human primate populations based on qPCR detection. The three populations studied were common chimpanzees (Pan troglodytes) in Senegal and gorillas (Gorilla gorilla) in the Republic of the Congo and in the Beauval Zoo (France). Blastocystis spp. were mainly found, with an occurrence close to 100%, followed by Balantidiumcoli (23.7%), Giardiaintestinalis (7.9%), Encephalitozoonintestinalis (1.3%) and Dientamoebafragilis (0.2%). None of the following protozoa were detected: Entamoebahistolytica, Enterocytozoonbieneusi, Cryptosporidiumparvum, C. hominis, Cyclosporacayetanensis or Cystoisosporabelli. As chimpanzees and gorillas are genetically close to humans, it is important to monitor them frequently against different pathogens to protect these endangered species and to assess potential zoonotic transmissions to humans.
Dose-Dependent Solubility–Permeability Interplay for Poorly Soluble Drugs under Non-Sink Conditions
Authors: Sugita Kazuya , Takata Noriyuki , Yonemochi Etsuo
Published: March 2021

We investigated the solubility–permeability interplay using a solubilizer additive under non-sink conditions. Sodium lauryl sulfate (SLS) was used as a solubilizer additive. The solubility and permeability of two poorly soluble drugs at various doses, with or without SLS, were evaluated by flux measurements. The total permeated amount of griseofulvin, which has high permeability, increased by the addition of SLS. On the other hand, triamcinolone, which has low permeability, showed an almost constant rate of permeation regardless of the SLS addition. The total permeated amount of griseofulvin increased by about 20–30% when the dose amount exceeded its solubility, whereas its concentration in the donor chamber remained almost constant. However, the total permeated amount of triamcinolone was almost constant regardless of dose amount. These results suggest that the permeability of the unstirred water layer (UWL) may be affected by SLS and solid drugs for high-permeable drugs. The effect of solid drugs could be explained by a reduction in the apparent UWL thickness. For the appropriate evaluation of absorption, it would be essential to consider these effects.
Could Bryophagous Beetles (Coleoptera: Byrrhidae) Help Us Understand Bryophyte Taxonomy? Preferences within the Hypnum cupressiforme Hedw. Species Complex
Authors: Pyszko Petr , Drgová Michaela , Ožana Stanislav , Dorňák Ondřej , Rožek David , Lee Číp Daniel , Plášek Vítězslav , Drozd Pavel
Published: March 2021

Intrataxonomic differences in terms of angiosperm suitability for herbivorous insects stem from variables such as plant structure, palatability, and chemistry. It has not yet been elucidated whether these differences also occur in terms of the bryophyte’s suitability to bryophages. Hypnum cupressiforme Hedw. is a morphologically variable moss species frequently inhabited or fed by insects. In this investigation, we offered five morphotypes of H. cupressiforme to two bryophagous species of Byrrhidae (Coleoptera) to reveal whether the intrataxonomic variability affects beetles’ preferences. The morphotypes were offered with preserved and removed spatial structures. There were no significant differences in morphotype preferences when spatial structures were preserved, although during the daytime, the beetles moved from the flat morphotype to the usual and turgid morphotypes. The beetles preferred the turgid morphotype when the spatial structures were removed. The results suggest that the spatial structure variations in the H. cupressiforme complex are accompanied by different chemical, physiological, or microscopic morphological profiles that are recognized by the bryophagous insects. Phylogenetic and epigenetic analyses can reveal multiple differences within the H. cupressiforme complex. Their interconnection with information about the preferences of bryophagous insects can help us to elucidate which of these differences are ecologically relevant.
DEM Study on the Segregation of a Non-Spherical Intruder in a Vibrated Granular Bed
Authors: Qiao Jinpeng , Dong Kejun , Duan Chenlong
Published: March 2021

The segregation process of a single large intruder in a vibrated bed of small particles has been widely studied, but most previous studies focused on spherical intruders. In this work, the discrete element method was used to study the effects of vibration conditions and intruder shape on the dimensionless ascending velocity (va) of the intruder. The intruder was in a prolate shape with aspect ratio varied but its equivalent diameter fixed. Three equivalent diameters, namely volume-equivalent diameter, surface-area-equivalent diameter, and Sauter diameter, were used. It was found that va increases and then decreases with the rise of the dimensionless vibration amplitude (Ad) and the dimensionless vibration frequency (fd), and va increases with the decrease of the sphericity of the intruder (Φ). Moreover, the porosity variation in the vibrated bed and the granular temperature were analyzed, which can be linked to the change of va. It was further found that va can be uniformly correlated to Ad·fd0.5, while the critical change of the response of va to Ad and fd occurs at Γ = 4.83, where Γ is the vibration intensity. Based on these findings, a piecewise equation was proposed to predict va as a function of Ad, fd, and Φ.
Risk Assessment for Personalized Health Insurance Based on Real-World Data
Authors: Pnevmatikakis Aristodemos , Kanavos Stathis , Matikas George , Kostopoulou Konstantina , Cesario Alfredo , Kyriazakos Sofoklis
Published: March 2021

The way one leads their life is considered an important factor in health. In this paper we propose a system to provide risk assessment based on behavior for the health insurance sector. To do so we built a platform to collect real-world data that enumerate different aspects of behavior, and a simulator to augment actual data with synthetic. Using the data, we built classifiers to predict variations in important quantities for the lifestyle of a person. We offer a risk assessment service to the health insurance professionals by manipulating the classifier predictions in the long-term. We also address virtual coaching by using explainable Artificial Intelligence (AI) techniques on the classifier itself to gain insights on the advice to be offered to insurance customers.
Model-Based Flow Rate Control with Online Model Parameters Identification in Automatic Pouring Machine
Authors: Kabasawa Nobutoshi , Noda Yoshiyuki
Published: March 2021

In this study, we proposed an advanced control system for tilting-ladle-type automatic pouring machines in the casting industry. Automatic pouring machines have been introduced recently to improve the working environment of the pouring process. In the conventional study on pouring control, it has been confirmed that the pouring flow rate control contributes to improving the accuracy of the entire automatic pouring machine, such as the outflow liquid’s falling position from the ladle, the liquid’s weight filled in the mold, and the sprue cup’s liquid level. However, the conventional control system has problems: it is not easy to precisely pour the liquid in the ladle with a large tilting angle, and it takes time to adjust the control parameters. Therefore, we proposed the feedforward pouring flow rate control system, constructed by the pouring process’ inverse model with the online model parameters identification. In this approach, we derived the pouring process’ mathematical model, representing precisely the pouring process with the ladle’s large tilting angle. The model parameters in the pouring process’ inverse model in the controller are updated online via the model parameters identification. To verify the proposed pouring control system’s efficacy, we experimented using the tilting-ladle-type automatic pouring machine. In the experimental results, the mean absolute error between the outflow liquid’s weight and the reference weight was improved from 0.1346 at the first pouring to 0.0498 at the fifth pouring. Moreover, the model parameters were identified within 4 s. Therefore, it enables updating the controller’s parameters within each pouring motion interval by the proposed approach.
Nitrogen Management Based on Visible/Near Infrared Spectroscopy in Pear Orchards
Authors: Wang Jie , Shi Xiaojun , Xu Yangchun , Dong Caixia
Published: March 2021

The timely estimation of nitrogen (N) requirements is essential for managing N fertilizer application in pear orchards. Visible/near infrared spectroscopy is a non-destructive and effective technique for real-time assessment of leaf N concentration, but its utility for decisions about fertilizer application in the pear orchards remains to be determined. In this study, we used leaf spectroscopy to determine leaf N concentration, used this value to calculate the amounts of N required for supplementary fertilization, and then evaluated the effects of the application. Over the two-year study, Cuiguan pear trees were treated with N at the following rates: 0 (N0), 100 (N1), 200 (N2), 300 (N3), and 400 (N4) g N per tree, regarded as five “controlled” N application rates. Another four “regulatory” treatments (Nr1-4) were fertilized as the “controlled” N application rates the first year, then given adjusted N application by topdressing as calculated using the N concentrations inferred from visible/near infrared spectroscopy data the second year. A model (R2 = 0.82) was established the first year to relate leaf spectra and N concentration using a partial least squares regression with full bands (350–2500 nm). The amount of N in the topdressing for the supplemental treatments was determined using the predicted leaf N concentration and the topdressing calculation method adapted from the N balance formula. Results showed that adjusted N applications of the Nr1 and Nr2 increased yield by 26% and 23%, respectively, over the controlled treatments N1 and N2. Although treatments Nr3 and Nr4 did not increase yield significantly over N3 and N4, the partial factor productivity of nitrogen in Nr4 was higher than the N4. The transverse diameter of fruit from Nr1 trees was significantly higher than from N1 trees, while the longitudinal diameter of fruit from Nr1, Nr2, and Nr3 trees was significantly higher than from N1, N2 and N3 trees, suggesting that fruit longitudinal growth and single-fruit weight is stimulated by adjusted N applications. However, the soluble solids in fruit from trees receiving adjusted N were not significantly greater than in fruit from non-supplemented trees. In conclusion, our results illustrate that regulatory N management contributes to fruit yield and quality especially in the nitrogen deficiency condition and improves the nitrogen use efficiency in nitrogen surplus. The N prediction model established using the nondestructive visible/near infrared spectroscopy is convenient and economical.
Galileo L10 Satellites: Orbit, Clock and Signal-in-Space Performance Analysis
Authors: Andrei Constantin-Octavian , Lahtinen Sonja , Poutanen Markku , Koivula Hannu , Johansson Jan
Published: March 2021

The tenth launch (L10) of the European Global Navigation Satellite System Galileo filled in all orbital slots in the constellation. The launch carried four Galileo satellites and took place in July 2018. The satellites were declared operational in February 2019. In this study, we report on the performance of the Galileo L10 satellites in terms of orbital inclination and repeat period parameters, broadcast satellite clocks and signal in space (SiS) performance indicators. We used all available broadcast navigation data from the IGS consolidated navigation files. These satellites have not been reported in the previous studies. First, the orbital inclination (56.7±0.15°) and repeat period (50680.7±0.22 s) for all four satellites are within the nominal values. The data analysis reveals also 13.5-, 27-, 177- and 354-days periodic signals. Second, the broadcast satellite clocks show different correction magnitude due to different trends in the bias component. One clock switch and several other minor correction jumps have occurred since the satellites were declared operational. Short-term discontinuities are within ±1 ps/s, whereas clock accuracy values are constantly below 0.20 m (root-mean-square—rms). Finally, the SiS performance has been very high in terms of availability and accuracy. Monthly SiS availability has been constantly above the target value of 87% and much higher in 2020 as compared to 2019. Monthly SiS accuracy has been below 0.20 m (95th percentile) and below 0.40 m (99th percentile). The performance figures depend on the content and quality of the consolidated navigation files as well as the precise reference products. Nevertheless, these levels of accuracy are well below the 7 m threshold (95th percentile) specified in the Galileo service definition document.
A 40 Mb/s VLC System Reusing an Existing Large LED Panel in an Indoor Office Environment
Authors: Li Xicong , Ghassemlooy Zabih , Zvánovec Stanislav , Haigh Paul Anthony
Published: March 2021

With advances in solid-state lighting, visible light communication (VLC) has emerged as a promising technology to enhance existing light-emitting diode (LED)-based lighting infrastructure by adding data communication capabilities to the illumination functionality. The last decade has witnessed the evolution of the VLC concept through global standardisation and product launches. Deploying VLC systems typically requires replacing existing light sources with new luminaires that are equipped with data communication functionality. To save the investment, it is clearly desirable to make the most of the existing illumination systems. This paper investigates the feasibility of adding data communication functionality to the existing lighting infrastructure. We do this by designing an experimental system in an indoor environment based on an off-the-shelf LED panel typically used in office environments, with the dimensions of 60 × 60 cm2. With minor modifications, the VLC function is implemented, and all of the modules of the LED panel are fully reused. A data rate of 40 Mb/s is supported at a distance of up to 2 m while using the multi-band carrierless amplitude and phase (CAP) modulation. Two main limiting factors for achieving higher data rates are observed. The first factor is the limited bandwidth of the LED string inside the panel. The second is the flicker due to the residual ripple of the bias current that is generated by the panel’s driver. Flicker is introduced by the low-cost driver, which provides bias currents that fluctuate in the low frequency range (less than several kilohertz). This significantly reduces the transmitter’s modulation depth. Concurrently, the driver can also introduce an effect that is similar to baseline wander at the receiver if the flicker is not completely filtered out. We also proposed a solution based on digital signal processing (DSP) to mitigate the flicker issue at the receiver side and its effectiveness has been confirmed.