bluuebunny/crossref_metadata_2025 huggingface dataset:
BAl4Mg−/0/+: Global Minima with a Planar Tetracoordinate or Hypercoordinate Boron Atom
Authors: Khatun Maya , Roy Saikat , Giri Sandip , CH Sasanka Sankhar Reddy , Anoop Anakuthil , Thimmakondu Venkatesan S.
Published: October 2021

We have explored the chemical space of BAl4Mg−/0/+ for the first time and theoretically characterized several isomers with interesting bonding patterns. We have used chemical intuition and a cluster building method based on the tabu-search algorithm implemented in the Python program for aggregation and reaction (PyAR) to obtain the maximum number of possible stationary points. The global minimum geometries for the anion (1a) and cation (1c) contain a planar tetracoordinate boron (ptB) atom, whereas the global minimum geometry for the neutral (1n) exhibits a planar pentacoordinate boron (ppB) atom. The low-lying isomers of the anion (2a) and cation (3c) also contain a ppB atom. The low-lying isomer of the neutral (2n) exhibits a ptB atom. Ab initio molecular dynamics simulations carried out at 298 K for 2000 fs suggest that all isomers are kinetically stable, except the cation 3c. Simulations carried out at low temperatures (100 and 200 K) for 2000 fs predict that even 3c is kinetically stable, which contains a ppB atom. Various bonding analyses (NBO, AdNDP, AIM, etc.) are carried out for these six different geometries of BAl4Mg−/0/+ to understand the bonding patterns. Based on these results, we conclude that ptB/ppB scenarios are prevalent in these systems. Compared to the carbon counter-part, CAl4Mg−, here the anion (BAl4Mg−) obeys the 18 valence electron rule, as B has one electron fewer than C. However, the neutral and cation species break the rule with 17 and 16 valence electrons, respectively. The electron affinity (EA) of BAl4Mg is slightly higher (2.15 eV) than the electron affinity of CAl4Mg (2.05 eV). Based on the EA value, it is believed that these molecules can be identified in the gas phase. All the ptB/ppB isomers exhibit π/σ double aromaticity. Energy decomposition analysis predicts that the interaction between BAl4−/0/+ and Mg is ionic in all these six systems.
Phenolic Compounds and Functional Beverages
Authors: Pérez-Gregorio Rosa
Published: October 2021

The rise in the prevalence of non-communicable diseases (NCDs) over the last few years has promoted the need to afford consumers with accurate health data on food and beverage products as part of their right to health [...]
Understanding the Role of Fibroblasts following a 3D Tumoroid Implantation for Breast Tumor Formation
Authors: Rijal Girdhari
Published: October 2021

An understanding of the participation and modulation of fibroblasts during tumor formation and growth is still unclear. Among many speculates, one might be the technical challenge to reveal the versatile function of fibroblasts in tissue complexity, and another is the dynamics in tissue physiology and cell activity. The histology of most solid tumors shows a predominant presence of fibroblasts, suggesting that tumor cells recruit fibroblasts for breast tumor growth. In this review paper, therefore, the migration, activation, differentiation, secretion, and signaling systems that are associated with fibroblasts and cancer-associated fibroblasts (CAFs) after implantation of a breast tumoroid, i.e., a lab-generated tumor tissue into an animal, are discussed.
Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology
Authors: An Young-Hyeon , Kim Su-Hwan
Published: October 2021

In this study, we proposed a simple and easy method for fabricating a three-dimensional (3D) structure that can recapitulate the morphology of a tissue surface and deliver biological molecules into complex-shaped target tissues. To fabricate the 3D hydrogel film structure, we utilized a direct tissue casting method that can recapitulate tissue structure in micro-/macroscale using polydimethylsiloxane (PDMS). A replica 3D negative mold was manufactured by a polyurethane acrylate (PUA)-based master mold. Then, we poured the catechol-conjugated alginate (ALG-C) solution into the mold and evaporated it to form a dried film, followed by crosslinking the film using calcium chloride. The ALG-C hydrogel film had a tensile modulus of 725.2 ± 123.4 kPa and maintained over 95% of initial weight after 1 week without significant degradation. The ALG-C film captured over 4.5 times as much macromolecule (FITC-dextran) compared to alginate film (ALG). The cardiomyoblast cells exhibited high cell viability over 95% on ALG-C film. Moreover, the ALG-C film had about 70% of surface-bound lentivirus (1% in ALG film), which finally exhibited much higher viral transfection efficiency of GFP protein to C2C12 cells on the film than ALG film. In conclusion, we demonstrated a 3D film structure of biofunctionalized hydrogel for substrate-mediated drug delivery, and this approach could be utilized to recapitulate the complex-shaped tissues.
Glycan Epitopes and Potential Glycoside Antagonists of DC-SIGN Involved in COVID-19: In Silico Study
Authors: Gao Meina , Li Hui , Ye Chenghao , Chen Kaixian , Jiang Hualiang , Yu Kunqian
Published: October 2021

Glycosylation is an important post-translational modification that affects a wide variety of physiological functions. DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin) is a protein expressed in antigen-presenting cells that recognizes a variety of glycan epitopes. Until now, the binding of DC-SIGN to SARS-CoV-2 Spike glycoprotein has been reported in various articles and is regarded to be a factor in systemic infection and cytokine storm. The mechanism of DC-SIGN recognition offers an alternative method for discovering new medication for COVID-19 treatment. Here, we discovered three potential pockets that hold different glycan epitopes by performing molecular dynamics simulations of previously reported oligosaccharides. The “EPN” motif, “NDD” motif, and Glu354 form the most critical pocket, which is known as the Core site. We proposed that the type of glycan epitopes, rather than the precise amino acid sequence, determines the recognition. Furthermore, we deduced that oligosaccharides could occupy an additional site, which adds to their higher affinity than monosaccharides. Based on our findings and previously described glycoforms on the SARS-CoV-2 Spike, we predicted the potential glycan epitopes for DC-SIGN. It suggested that glycan epitopes could be recognized at multiple sites, not just Asn234, Asn149 and Asn343. Subsequently, we found that Saikosaponin A and Liquiritin, two plant glycosides, were promising DC-SIGN antagonists in silico.
Heat Shock Proteins HSPA1 and HSP90AA1 Are Upregulated in Colorectal Polyps and Can Be Targeted in Cancer Cells by Anti-Inflammatory Oxicams with Arylpiperazine Pharmacophore and Benzoyl Moiety Substitutions at Thiazine Ring
Authors: Szczuka Izabela , Wierzbicki Jarosław , Serek Paweł , Szczęśniak-Sięga Berenika M. , Krzystek-Korpacka Małgorzata
Published: October 2021

Heat shock proteins HSPA1/Hsp70α and HSP90AA1/Hsp90α are crucial for cancer growth but their expression pattern in colorectal polyps or whether they can be modulated by oxicams is unknown. We quantified (RTqPCR) HSPA1 and HSP90AA1 expression in 50 polyp-normal pairs in relation to polyp malignancy potential and examined the effect of piroxicam, meloxicam and five novel analogues on HSPA1 and HSP90AA1 expression (mRNA/protein) in colorectal adenocarcinoma lines. HSPA1 and HSP90AA1 were upregulated in polyps by 3- and 2.9-fold. Expression ratios were higher in polyps with higher dysplasia grade and dominant villous growth pattern, mostly a result of diminished gene expression in normal tissue. Classic oxicams had negligible/non-significant effect on HSP expression. Their most effective analogue inhibited HSPA1 protein and gene by 2.5-fold and 5.7-fold in Caco-2 and by 11.5-fold and 6.8-fold in HCT116 and HSPA1 protein in HT-29 by 1.9-fold. It downregulated HSP90AA1 protein and gene by 1.9-fold and 3.7-fold in Caco-2 and by 2-fold and 5.0-fold in HCT116. HSPA1 and HSP90AA1 are upregulated in colorectal polyps reflecting their potential for malignancy. HSPA1 in cancer cells and, to lesser degree, HSP90AA1 can be reduced by oxicam analogues with thiazine ring substituted via propylene linker by arylpiperazine pharmacophore with fluorine substituents and by benzoyl moiety.
Metabolic Dysfunction Biomarkers as Predictors of Early Diabetes
Authors: Luís Carla , Baylina Pilar , Soares Raquel , Fernandes Rúben
Published: October 2021

During the pathophysiological course of type 2 diabetes (T2D), several metabolic imbalances occur. There is increasing evidence that metabolic dysfunction far precedes clinical manifestations. Thus, knowing and understanding metabolic imbalances is crucial to unraveling new strategies and molecules (biomarkers) for the early-stage prediction of the disease’s non-clinical phase. Lifestyle interventions must be made with considerable involvement of clinicians, and it should be considered that not all patients will respond in the same manner. Individuals with a high risk of diabetic progression will present compensatory metabolic mechanisms, translated into metabolic biomarkers that will therefore show potential predictive value to differentiate between progressors/non-progressors in T2D. Specific novel biomarkers are being proposed to entrap prediabetes and target progressors to achieve better outcomes. This study provides a review of the latest relevant biomarkers in prediabetes. A search for articles published between 2011 and 2021 was conducted; duplicates were removed, and inclusion criteria were applied. From the 29 studies considered, a survey of the most cited (relevant) biomarkers was conducted and further discussed in the two main identified fields: metabolomics, and miRNA studies.
Stable Gastric Pentadecapeptide BPC 157 as a Therapy for the Disable Myotendinous Junctions in Rats
Authors: Japjec Mladen , Horvat Pavlov Katarina , Petrovic Andreja , Staresinic Mario , Sebecic Bozidar , Buljan Matko , Vranes Hrvoje , Giljanovic Ana , Drmic Domagoj , Japjec Miroslav , Prtoric Andreja , Lovric Eva , Batelja Vuletic Lovorka , Dobric Ivan , Boban Blagaic Alenka , Skrtic Anita , Seiwerth Sven , Predrag Sikiric
Published: October 2021

(1) Aim: The stable gastric pentadecapeptide BPC 157 is known to heal transected muscle, tendon, and ligament. Thereby, in this study, we investigated the effect of BPC 157 on the dissection of the quadriceps tendon from the quadriceps muscle in rats. (2) Materials and Methods: Myotendinous junction defect, which cannot heal spontaneously in rats, as evidenced with consistent macro/microscopic, biomechanical, functional assessments, eNOS, and COX-2 mRNA levels and oxidative stress and NO-levels in the myotendinous junctions. BPC 157 (10 µg/kg, 10 ng/kg) regimen was given (i) intraperitoneally, first application immediately after surgery, last 24 h before sacrifice; (ii) per-orally, in drinking water (0.16 µg/mL, 0.16 ng/mL, 12 mL/rat/day), till the sacrifice at 7, 14, 28 and 42 postoperative days. (3) Results: These BPC 157 regimens document prominent therapy effects (macro/microscopic, biomechanical, functional much like eNOS and COX-2 mRNA levels and counteracted oxidative stress and NO-levels in the myotendinous junctions), while controls have a poor presentation. Especially, in rats with the disabled myotendinous junction, along with full functional recovery, BPC 157 counteracts muscle atrophy that is regularly progressive and brings muscle presentation close to normal. Accordingly, unlike the perilous course in controls, those rats, when receiving BPC 157 therapy, exhibit a smaller defect, and finally defects completely disappear. Microscopically, there are no more inflammatory infiltrate, well-oriented recovered tissue of musculotendon junction appears in BPC 157 treated rats at the 28 days and 42 days. (4) Conclusions: BPC 157 restores myotendinous junction in accordance with the healing of the transected muscle, tendon, and ligament.
Gene Mutations in Circulating Tumour DNA as a Diagnostic and Prognostic Marker in Head and Neck Cancer—A Systematic Review
Authors: Hudečková Markéta , Koucký Vladimír , Rottenberg Jan , Gál Břetislav
Published: October 2021

(1) Background: Head and Neck Squamous Cell Carcinoma (HNSCC) is one of the most common malignancies globally. An early diagnosis of this disease is crucial, and the detection of gene mutations in circulating tumour DNA (ctDNA) through a liquid biopsy is a promising non-invasive diagnostic method. This review aims to provide an overview of ctDNA mutations in HNSCC patients and discuss the potential use of this tool in diagnosis and prognosis. (2) Methods: A systematic search for articles published in the English language between January 2000 and April 2021 in the Medline and Scopus databases was conducted. (3) Results: A total of 10 studies published in nine publications were selected and analysed. Altogether, 390 samples were obtained from HNSCC patients, and 79 control samples were evaluated. The most often explored gene mutation in ctDNA was TP53. (4) Conclusions: The examination of a larger group of gene mutations and the use of a combination of multiple detection methods contribute to a higher detection rate of mutated ctDNA. More studies are necessary to verify these conclusions and to translate them into clinical practice.
Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice
Authors: Albracht-Schulte Kembra , Wilson Savanna , Johnson Paige , Pahlavani Mandana , Ramalingam Latha , Goonapienuwala Bimba , Kalupahana Nishan S. , Festuccia William T. , Scoggin Shane , Kahathuduwa Chanaka N. , Moustaid-Moussa Naima
Published: October 2021

Visceral obesity may be a driving factor in nonalcoholic fatty liver disease (NAFLD) development. Previous studies have shown that the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA), ameliorates obesity in high-fat (HF) fed male, C57Bl/6 mice at thermoneutral conditions, independent of uncoupling protein 1 (UCP1). Our goals herein were to investigate sex-dependent mechanisms of EPA in the livers of wild type (WT) and UCP1 knockout (KO) male and female mice fed a HF diet (45% kcal fat; WT-HF, KO-HF) with or without supplementation of 36 g/kg EPA (WT-EPA, KO-EPA). KO significantly increased body weight in males, with no significant reductions with EPA in the WT or KO groups. In females, there were no significant differences in body weight among KO groups and no effects of EPA. In males, liver TGs were significantly higher in the KO-HF group and reduced with EPA, which was not observed in females. Accordingly, gene and protein markers of mitochondrial oxidation, peroxisomal biogenesis and oxidation, as well as metabolic futile cycles were sex-dependently impacted by KO and EPA supplementation. These findings suggest a genotypic difference in response to dietary EPA supplementation on the livers of male and female mice with diet-induced obesity and housed at thermoneutrality.
Tuning of Hydrogel Architectures by Ionotropic Gelation in Microfluidics: Beyond Batch Processing to Multimodal Diagnostics
Authors: Smeraldo Alessio , Ponsiglione Alfonso Maria , Netti Paolo Antonio , Torino Enza
Published: October 2021

Microfluidics is emerging as a promising tool to control physicochemical properties of nanoparticles and to accelerate clinical translation. Indeed, microfluidic-based techniques offer more advantages in nanomedicine over batch processes, allowing fine-tuning of process parameters. In particular, the use of microfluidics to produce nanoparticles has paved the way for the development of nano-scaled structures for improved detection and treatment of several diseases. Here, ionotropic gelation is implemented in a custom-designed microfluidic chip to produce different nanoarchitectures based on chitosan-hyaluronic acid polymers. The selected biomaterials provide biocompatibility, biodegradability and non-toxic properties to the formulation, making it promising for nanomedicine applications. Furthermore, results show that morphological structures can be tuned through microfluidics by controlling the flow rates. Aside from the nanostructures, the ability to encapsulate gadolinium contrast agent for magnetic resonance imaging and a dye for optical imaging is demonstrated. In conclusion, the polymer nanoparticles here designed revealed the dual capability of enhancing the relaxometric properties of gadolinium by attaining Hydrodenticity and serving as a promising nanocarrier for multimodal imaging applications.
Laboratory Measurement and Boundary Conditions for the Water Vapour Resistivity Properties of Typical Australian Impermeable and Smart Pliable Membranes
Authors: Olaoye Toba Samuel , Dewsbury Mark , Künzel Hartwig
Published: October 2021

The duo of better insulated and more air-tight envelopes without appropriate consideration of water vapour diffusion and envelope moisture management has often demonstrated an increased potential of moisture accumulation, interstitial condensation, and mould growth within the building envelope. To inform a resilient, energy efficient, and healthy building design, long-term transient hygrothermal modelling are required. Since 2008, concern has been raised to the Australian building regulators regarding the need to establish the vapour diffusion properties of construction materials, in order to develop a hygrothermal regulatory framework. This paper discusses the results from laboratory testing of the vapour diffusion properties of two common reflective pliable membranes, and one smart pliable membrane. The two reflective pliable membranes are often used within the exterior walls of Australian buildings. The smart pliable membrane is a relatively new, internationally available product. The three membranes were tested as per ISO 12,572 at 23 °C and 50% RH. To establish if the vapour resistivity properties were constant, under different relative humidity conditions, the membranes were further tested at 23 °C and relative humidity values of 35%, 65%, and 80%. The results of the three pliable membranes show that the vapour resistivity properties varied in a non-linear (dynamic) manner subject to relative humidity. In conclusion, this research demonstrates that regardless of the class, each of the tested membrane types behaved differently under varying relative humidity and pressure gradients within the testing laboratory.
Assessing Value in Child Health
Authors: Ungar Wendy J.
Published: October 2021

Assessing value in child health is increasingly important as health care systems face difficult choices with regard to what services and programs for children to fund and deliver [...]
Diagnosing Malaria Patients with Plasmodium falciparum and vivax Using Deep Learning for Thick Smear Images
Authors: Kassim Yasmin M. , Yang Feng , Yu Hang , Maude Richard J. , Jaeger Stefan
Published: October 2021

We propose a new framework, PlasmodiumVF-Net, to analyze thick smear microscopy images for a malaria diagnosis on both image and patient-level. Our framework detects whether a patient is infected, and in case of a malarial infection, reports whether the patient is infected by Plasmodium falciparum or Plasmodium vivax. PlasmodiumVF-Net first detects candidates for Plasmodium parasites using a Mask Regional-Convolutional Neural Network (Mask R-CNN), filters out false positives using a ResNet50 classifier, and then follows a new approach to recognize parasite species based on a score obtained from the number of detected patches and their aggregated probabilities for all of the patient images. Reporting a patient-level decision is highly challenging, and therefore reported less often in the literature, due to the small size of detected parasites, the similarity to staining artifacts, the similarity of species in different development stages, and illumination or color variations on patient-level. We use a manually annotated dataset consisting of 350 patients, with about 6000 images, which we make publicly available together with this manuscript. Our framework achieves an overall accuracy above 90% on image and patient-level.
Event-Triggered Fixed-Time Integral Sliding Mode Control for Nonlinear Multi-Agent Systems with Disturbances
Authors: Li Xue , Yu Zhiyong , Jiang Haijun
Published: October 2021

In this paper, the leader-following consensus problem of first-order nonlinear multi-agent systems (FONMASs) with external disturbances is studied. Firstly, a novel distributed fixed-time sliding mode manifold is designed and a new static event-triggered protocol over general directed graph is proposed which can well suppress the external disturbances and make the FONMASs achieve leader-following consensus in fixed-time. Based on fixed-time stability theory and inequality technique, the conditions to be satisfied by the control parameters are obtained and the Zeno behavior can be avoided. In addition, we improve the proposed protocol and propose a new event-triggering strategy for the FONMASs with multiple leaders. The systems can reach the sliding mode surface and achieve containment control in fixed-time if the control parameters are designed carefully. Finally, several numerical simulations are given to show the effectiveness of the proposed protocols.
Identifying the Potential of RFID in Disaster Healthcare: An International Delphi Study
Authors: Madanian Samaneh , Parry Dave
Published: October 2021

Mainstream healthcare has been facing numerous challenges, and it is expected to see that these challenges become more severe and frequent when healthcare is dealing with disasters. This points to the necessity of utilising technologies to support healthcare and disaster managers in making quality decisions during chaotic and rapidly changing conditions in disaster situations. Therefore, in this research, the objective is to identify the role of RFID technology in healthcare-related activities before, during, and after disasters in terms of application areas and phases of the disaster management cycle (DMC). A Delphi approach was used in this research. Two rounds of questionnaires were administered to a panel of experts to evaluate the actual and potential use of RFID applications for healthcare within DMC. The Delphi participants were the field experts in the areas of disaster management, disaster medicine, and information systems. Based on the Delphi results, RFID applications were seen to be most useful in the response and recovery phases of disasters. RFID was seen as being most helpful for health-related supply management and casualty information. There were concerns that privacy and security may be barriers to adoption and use. Other applications identified by this study include identifying and tracking medical resources (including clinicians and first responders) and their accurate coordination in the response missions, determining idle resources, and maximising their utilisation during response activities. In this research, 35 potential scenarios of RFID applications for healthcare purposes within DMC and Disaster e-Health (DEH) were evaluated with the Delphi participants. RFID technologies could play an important role in DMC and DEH to provide more reliable and timely information to support healthcare during disasters. Based on the research results, managing the supply chain emerged as a major RFID application for supporting disaster healthcare.
An Adaptive Protection Scheme for Coordination of Distance and Directional Overcurrent Relays in Distribution Systems Based on a Modified School-Based Optimizer
Authors: Abdelhamid Mohamed , Kamel Salah , Korashy Ahmed , Tostado-Véliz Marcos , Banakhr Fahd A , Mosaad Mohamed I.
Published: October 2021

This paper presents an adaptive protection scheme (APS) for solving the coordination problem that deals with coordination directional overcurrent relays (DOCRs) and distance relays second zone time, in relation to coordination with DOCRs. The coordination problem becomes more complex with the impact of renewable energy sources (RES) when added to the distribution grid. This leads to a change in the grid topology, caused by the on/off states of the distribution generators (DG). The frequency of topological changes in distribution grids poses a challenge to the power system’s protection components. The change in the state of DGs leads to malfunction in reliability and miscoordination between protection relays, since that causes a direct effect to the short circuit currents. This paper used the school-based optimization (SBO) algorithm, which simulates the educational process, in order to deal with coordination problems. That algorithm is modified (MSBO) by modified both learning and teaching processes. The IEEE 8-bus test system and IEEE 14-bus distribution network are used to validate the proposed coordination system’s effectiveness when dealing with the coordination process between distance and DOCRs, at both the near- and far-end in the typical topological grid and with DGs in working order.
Justification of the Energy Use of Cedar Husk Waste as an Environmentally Friendly Additive for Co-Combustion with Coal
Authors: Stanislav Yankovsky , Anton Tolokol’nikov , Nikolay Berezikov , Vladimir Gubin
Published: October 2021

In this paper, the properties of ignition of mixed fuel pellets formed on the basis of fairly typical energy coal and wood industry waste in the form of cedar husks are experimentally established. The technical characteristics of the initial fuel components and the mixtures based on them, the ignition delay times for different mass concentrations of biomass in coal, and the composition of flue gases formed during the thermal decomposition of these mixed fuels and their base components were determined. Pellets of mixed fuels were made by a hydraulic press. The experiments were performed in an air environment at temperatures from 600 °C to 800 °C. Recording of the processes of pellet ignition and combustion was carried out using a high-speed video camera with an image format of 1024 × 1024 pixels, and a frame rate up to 500 frames per second. The analysis of the flue gas composition was performed using a Test-1 factory gas analyzer (BONER Co.). It was found that the increase in the share of biomass up to 50% in the mixed fuel led to a significant reduction in the ignition delay time to less than 1 s and the sequestration of sulfur oxide emissions by 37.6% and of nitrogen oxides by 3.8% in the studied granular mixed fuels.
SCADA Data-Based Working Condition Classification for Condition Assessment of Wind Turbine Main Transmission System
Authors: Chen Huanguo , Xie Chao , Dai Juchuan , Cen Enjie , Li Jianmin
Published: October 2021

Due to the complex and variable conditions under which wind turbines operate, existing working condition classification methods are inadequate for condition assessment of the main transmission system. Because working conditions are too few after classification, it cannot effectively describe the complex and variable working conditions of wind turbine. This can lead to high false-alarm rates in the condition monitoring, which affect normal operations. This paper proposes a working condition classification method for the main transmission system of wind turbines based on supervisory control and data acquisition (SCADA) data. Firstly, correlation analysis of SCADA data acquired by wind farm is used to select the parameters relevant to the main transmission system. Secondly, according to the wind turbine control principle, the working conditions are initially divided into four phases: shutdown, start-up, maximum wind energy tracking, and constant speed. The k-means clustering algorithm is used to subdivide the maximum wind energy-tracking phase and constant speed phase, which account for a larger proportion of the working conditions, to achieve better classification. Finally, a case study is used to demonstrate the calculation of alarm thresholds and alarm rates for each working condition. The results are compared with the direct use of k-means clustering for working condition classification. It is concluded that the proposed method can significantly reduce the false-alarm rate of the vibration detection process.
Labeling of Installed Heating Appliances in Residential Buildings: An Energy Labeling Methodology for Improving Consumers’ Awareness
Authors: Menegon Diego , Lobosco Daniela , Micò Leopoldo , Fernandes Joana
Published: October 2021

In the EU 28, the installed heating appliance stock is quite old, with an actual replacement rate of 4%. This is directly reflected in the average efficiency of the installed heating systems, where around 60% of the stock is rated with an energy class of C or D (the lowest classes of the energy label scale). The European project HARP aims at raising consumers’ awareness of the planned replacement of their old and inefficient heating appliances with more efficient and renewable solutions. In this direction, an energy labeling methodology for old appliances has been developed to rate the installed stock before the introduction of the EU energy label. The methodology has been developed for space heating appliances and water heaters, targeting two types of users: end consumers and professional users. The validation considered about 4600 space heating appliances and 800 water heaters built between 1972 and 2019. Three heating appliances and two water heaters were tested in the laboratory, confirming the reliability of the proposed methodology. The expected impact of defining an energy labeling methodology for installed heating appliances increases the current replacement rate of these appliances in the EU from 4% to 5%.
A Typology of Food Environments in the Pacific Region and Their Relationship to Diet Quality in Solomon Islands
Authors: Bogard Jessica R. , Andrew Neil L. , Farrell Penny , Herrero Mario , Sharp Michael K. , Tutuo Jillian
Published: October 2021

Extensive literature describes the importance of food environments (FEs) as a driver of food choices and nutrition outcomes; yet existing FE frameworks do not adequately capture the diversity of FEs relevant to the Pacific Region. This limits identification of opportunities in food systems to reduce the multiple burden of malnutrition. We present a conceptual typology of FEs including six primary FEs relevant in the Pacific; wild; cultivated; kin and community; informal retail; formal retail; and food aid and services. We then apply this typology to food acquisition data from Solomon Islands 2012/13 Household Income and Expenditure Survey and analyse the relationship between FEs and diet quality. The cultivated FE accounts for 60% of the quantity of food acquired nationally, followed by wild (15%), kin and community (9%), and formal and informal retail FEs (8% each), with wide variation between urban and rural households, provinces and wealth groups. Reliance on different FEs is a significant predictor of diet quality and affirms the importance of subsistence fisheries and agriculture, and community and kinship networks. Integration of a FE typology such as the one presented here in commonly conducted household expenditure surveys offers significant opportunity to advance our understanding of food system leverage points to improve nutrition and health.
Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
Authors: Hong Liping , Pan Mingfei , Xie Xiaoqian , Liu Kaixin , Yang Jingying , Wang Shan , Wang Shuo
Published: October 2021

Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.
Analysis of Phytonutrients, Anti-Mutagenic and Chemopreventive Effects of Tropical Fruit Extracts
Authors: Temviriyanukul Piya , Kittibunchakul Suwapat , Trisonthi Piyapat , Inthachat Woorawee , Siriwan Dalad , Suttisansanee Uthaiwan
Published: October 2021

Thailand is located in the tropics and a wide variety of fruits are grown commercially. However, studies regarding the phytonutrients, anti-mutagenic and chemopreventive effects of these fruits are limited. Thus, phytochemical profiles and inhibition of key enzymes involved in obesity and diabetes, together with anti-mutagenic and chemopreventive properties of eight tropical fruit extracts cultivated in Thailand, including Psidium guajava ‘Kimju’, Psidium guajava ‘Keenok’, Ananas comosus ‘Pattavia’, Ananas comosus ‘Phulae’, Durio zibethinus ‘Chanee’, Durio zibethinus ‘Monthong’, Carica papaya ‘Khaekdum’ and Mangifera indica ‘Namdokmai’ were investigated. Different cultivars were also compared. Results showed that M. indica ‘Namdokmai’ was the most antioxidant-rich extract containing abundant 4-hydroxybenzoic acid and its derivative, gallic acid, as the main phenolics. M. indica ‘Namdokmai’ also exhibited high inhibitory capacities (>60% inhibition under studied conditions) against lipase, α-amylase and α-glucosidase, key enzymes as drug targets for controlling obesity and type 2 diabetes. Interestingly, all fruit extracts suppressed food mutagen-induced DNA mutations assayed by the Ames test, especially M. indica ‘Namdokmai’ and C. papaya ‘Khaekdum’ (>50% inhibition at 200 µg/plate). The M. indica ‘Namdokmai’ was also the most potent extract for suppression of cancer promotion (>90% inhibition at 200 µg/mL) followed by P. guajava ‘Kimju’, P. guajava ‘Keenok’ and C. papaya ‘Khaekdum’. Results potentially indicated that fruit intake after overcooked meat consumption might supplement nutrients and fiber and also reduce DNA mutation sources.
Effects of Roasting Sweet Potato (Ipomoea batatas L. Lam.): Quality, Volatile Compound Composition, and Sensory Evaluation
Authors: Tsai Yu-Jung , Lin Li-Yun , Yang Kai-Min , Chiang Yi-Chan , Chen Min-Hung , Chiang Po-Yuan
Published: October 2021

Roasting can increase the Maillard reaction and caramelization of sweet potatoes to create an attractive appearance, color, aroma, and taste, and is rapidly increasing in the commercial market. This study mainly analyzed the influence of roasting sweet potatoes, with and without the peel, on sweet potato quality and flavor characteristics combined with sensory qualities. The results showed that the a* value (1.65–8.10), browning degree (58.30–108.91), total acidity (0.14–0.21 g/100 g, DW), and maltose content (0.00–46.16 g/100 g, DW) of roasted sweet potatoes increased with roasting time. A total of 46 volatile compounds were detected and 2-furanmethanol, furfural, and maltol were identified as the main sources of the aroma of roasted sweet potatoes. A sensory evaluation based on a comprehensive nine-point acceptance test and descriptive analysis showed that roasting for 1 to 2 h resulted in the highest acceptance score (6.20–6.65), including a golden-yellow color, sweet taste, and fibrous texture. The sweet potatoes became brown after roasting for 2.5 to 3 h and gained a burnt and sour taste, which reduced the acceptance score (4.65–5.75). These results can provide a reference for increased quality in the food industry production of roasted sweet potatoes.
Comparison of the Proximate Composition and Nutritional Profile of Byproducts and Edible Parts of Five Species of Shrimp
Authors: Liu Zhenyang , Liu Qiumei , Zhang Di , Wei Shuai , Sun Qinxiu , Xia Qiuyu , Shi Wenzheng , Ji Hongwu , Liu Shucheng
Published: October 2021

The nutritional components of different parts (meat, head, shell and tail) of Litopenaeus vannamei (L.v), Macrobrachium rosenbergii (M.r), Penaeus monodon (P.m), Fenneropenaeus chinensis (F.c), and Penaeus japonicus (P.j) were analyzed and their nutritional values were evaluated. For the five species of shrimp, the meat yield was 37.47–55.94%, and the byproduct yield was 44.06–62.53%. The meat yields of L.v and F.c were the highest (55.94 and 55.92%, respectively), and the meat yield of M.r was the lowest (37.47%). The shrimp contain high amounts of crude protein, and the values of the amino acid score (AAS), chemical score (CS), and essential amino index (EAAI) were greater than or close to 1.00, indicating that shrimp protein had higher nutritional value. The shrimp head was rich in polyunsaturated fatty acids and the ratio of n-6 to n-3 PUFAs was from 0.37 to 1.68, indicating that the shrimp head is rich in n-3 PUFAs and is a good source of n-3 PUFAs. The five species of shrimp were rich in macro- and micro-minerals, especially in shrimp byproducts. The shrimp byproducts were also rich in other bioactive ingredients (astaxanthin), which are also very valuable for developing biological resources. Therefore, shrimp have many nutritional benefits, and their byproducts can also be used to develop natural nutraceuticals, which are considered to be one of the healthiest foods.
GRB Polarization: A Unique Probe of GRB Physics
Authors: Gill Ramandeep , Kole Merlin , Granot Jonathan
Published: October 2021

Over half a century from the discovery of gamma-ray bursts (GRBs), the dominant radiation mechanism responsible for their bright and highly variable prompt emission remains poorly understood. Spectral information alone has proven insufficient for understanding the composition and main energy dissipation mechanism in GRB jets. High-sensitivity polarimetric observations from upcoming instruments in this decade may help answer such key questions in GRB physics. This article reviews the current status of prompt GRB polarization measurements and provides comprehensive predictions from theoretical models. A concise overview of the fundamental questions in prompt GRB physics is provided. Important developments in gamma-ray polarimetry including a critical overview of different past instruments are presented. Theoretical predictions for different radiation mechanisms and jet structures are confronted with time-integrated and time-resolved measurements. The current status and capabilities of upcoming instruments regarding the prompt emission are presented. The very complimentary information that can be obtained from polarimetry of X-ray flares as well as reverse-shock and early to late forward-shock (afterglow) emissions are highlighted. Finally, promising directions for overcoming the inherent difficulties in obtaining statistically significant prompt-GRB polarization measurements are discussed, along with prospects for improvements in the theoretical modeling, which may lead to significant advances in the field.
Heraldry in the Republic of Macedonia (1991–2019)
Authors: Jonovski Jovan
Published: October 2021

Every European country now has some distinctive heraldic conventions and traditions embodied in the designs and artistic representations of the emblems forming part of its national corpus. This paper deals with these matters in the period from independence in 1991 to the recent change of name in 2019. It deals with the successive designs proposed for the emblem of the state itself, some of which conformed to international heraldic conventions closely enough to be called “arms” or “coats of arms”, not including the emblem adopted in 2009. Special attention is given to the distinctive conventions created for municipal heraldry, including its novel legal framework, as well as those governing personal heraldry developed in the twenty-first century. The paper examines the evolution of heraldic thought and practice in Macedonia in the three decades in question, especially in the context of the Macedonian Heraldic Society and its journal, The Macedonian Herald, and its Register of Arms and the Civic Heraldic System it created.
Deep-Water Accumulation of Volcaniclastic Detritus from a Petrographic Point of View: Beginning a Discussion from the Alpine Peripheral Basins
Authors: Di Capua Andrea , Barilaro Federica , Groppelli Gianluca
Published: October 2021

The interpretation of eruptive mechanisms accumulating ancient submarine volcaniclastic sequences is still extremely challenging, particularly when no spatial nor temporal constraints are identifiable. The present work reviews petrographic results gained during the last few decades on three different Paleogene Formations accumulated around the Alpine and Apennine Mountain belts, discussing how their detritus could have been formed and moved from the volcanic centers to the depo-centers, taking into account the volcanic mechanisms which are at the base of the production, transportation and accumulation of volcaniclastic detritus. In doing this, we reconsider the classical diagrams of Folk and Gazzi–Dickinson, rediscussing their significance on the basis of how orogenic volcanism delivers detritus to the environment. In addition, this work highlights the need of the scientific community for gaining new petrographic data on modern sedimentary systems to better constrain interpretative criteria for the petrographic study of ancient volcano–sedimentary sequences.
Towards Validating the Effectiveness of Obstructive Sleep Apnea Classification from Electronic Health Records Using Machine Learning
Authors: Ramesh Jayroop , Keeran Niha , Sagahyroon Assim , Aloul Fadi
Published: October 2021

Obstructive sleep apnea (OSA) is a common, chronic, sleep-related breathing disorder characterized by partial or complete airway obstruction in sleep. The gold standard diagnosis method is polysomnography, which estimates disease severity through the Apnea-Hypopnea Index (AHI). However, this is expensive and not widely accessible to the public. For effective screening, this work implements machine learning algorithms for classification of OSA. The model is trained with routinely acquired clinical data of 1479 records from the Wisconsin Sleep Cohort dataset. Extracted features from the electronic health records include patient demographics, laboratory blood reports, physical measurements, habitual sleep history, comorbidities, and general health questionnaire scores. For distinguishing between OSA and non-OSA patients, feature selection methods reveal the primary important predictors as waist-to-height ratio, waist circumference, neck circumference, body-mass index, lipid accumulation product, excessive daytime sleepiness, daily snoring frequency and snoring volume. Optimal hyperparameters were selected using a hybrid tuning method consisting of Bayesian Optimization and Genetic Algorithms through a five-fold cross-validation strategy. Support vector machines achieved the highest evaluation scores with accuracy: 68.06%, sensitivity: 88.76%, specificity: 40.74%, F1-score: 75.96%, PPV: 66.36% and NPV: 73.33%. We conclude that routine clinical data can be useful in prioritization of patient referral for further sleep studies.
Effects of a Randomized Home-Based Quality of Movement Protocol on Function, Posture and Strength in Outpatients with Obesity
Authors: Cavaggioni Luca , Gilardini Luisa , Redaelli Gabriella , Croci Marina , Capodaglio Paolo , Gobbi Michele , Bertoli Simona
Published: October 2021

The aim of this study was to determine the effects of two different home-based training interventions on functional parameters and body composition in obese patients. Sixty-four obese patients were recruited at the IRCCS Istituto Auxologico Italiano and randomly assigned into a movement quality group (MQ) and a conventional training group (CT). In the MQ, the training protocol combined various stimuli based on whole-body movement patterns, mobility, motor control and diaphragmatic breathing. The CT included traditional bodyweight resistance-training exercises. All patients were tested for movement efficiency (Functional Movement Screen, FMS), postural control (Modified Balance Error Scoring System, M-BESS), breathing pattern (Total Faulty Breathing Scale, TFBS), muscular strength (Handgrip Strength Test, HST and Five Repetition Sit to Stand, FRSTS) and body composition (Waist Circumference, WC, Body Mass Index, BMI, Body fat mass percentage, Fat Mass) before and after a 6-week period of training. Significant interactions and main effects of time (p < 0.0001) were found in MQ compared to CT in the FMS, M-BESS and TFBS parameters, while muscular strength (HST, FRSTS) and body composition parameters improved similarly in both groups with a main effect of time (p < 0.05). These findings suggest that a 6-week movement quality training is effective in ameliorating postural control and movement efficiency with similar improvements in muscular strength and body composition compared with a mere traditional home-based training. Fitness coaches and practitioners might consider the MQ intervention as a valuable alternative to conventional training when treating obesity.
Adult Granulosa Cell Tumor in Pregnancy: A New Case and a Review of the Literature
Authors: Guidi Sofia , Berghella Vincenzo , Scambia Giovanni , Fagotti Anna , Vidiri Annalisa , Restaino Stefano , Vizzielli Giuseppe , Inzani Frediano , Cavaliere Anna Franca
Published: October 2021

Granulosa cell tumors are rare ovarian tumors that can arise during pregnancy. We present a new case of recurrent adult granulosa cell tumor (AGCT) in pregnancy and a systematic review of the literature. The new case described is a 41-year-old woman G5P1122 with a prior history of AGCT that was referred to our center at 29 weeks because of a symptomatic abdominal mass, compatible with a possible recurrence of AGCT. At 36 + 3 weeks, she underwent a cesarean delivery for preterm labor and a total hysterectomy with a radical surgical staging. A healthy female infant was delivered. The patient received a platinum-based chemotherapy, with a 26-month follow-up negative for recurrence. Analyzing our case with the four identified by the literature review, three were recurrent and two were primary AGCT. Only one required surgery for AGCT at 15 weeks, while another underwent chemotherapy in pregnancy. In the other three cases, surgery for AGCT was done at the time of cesarean delivery. There were three cases of preterm delivery. All five pregnancies resulted in the birth of live babies with weight adequate for gestational age. In conclusion, AGCT diagnosed in pregnancy is rare, reported in only five cases. All gave birth to live babies in the third trimester, and maternal outcome at up to 18 months showed no recurrence.