bluuebunny/crossref_metadata_2025 huggingface dataset:
High nitrate levels in skeletal muscle contribute to nitric oxide generation via a nitrate/nitrite reductive pathway in mice that lack the nNOS enzyme
Authors: Upanan Supranee , Lee Jeeyoung , Tunau-Spencer Khalid J. , Rajvanshi Praveen K. , Wright Elizabeth C. , Noguchi Constance T. , Schechter Alan N.
Published: May 2024

Introduction:

Nitric oxide (NO) is a vasodilator gas that plays a critical role in mitochondrial respiration and skeletal muscle function. NO is endogenously generated by NO synthases: neuronal NO synthase (nNOS), endothelial NO synthase (eNOS), or inducible NO synthase (iNOS). NO in skeletal muscle is partly generated by nNOS, and nNOS deficiency can contribute to muscular dystrophic diseases. However, we and others discovered an alternative nitrate/nitrite reductive pathway for NO generation: nitrate to nitrite to NO. We hypothesized that nitrate supplementation would increase nitrate accumulation in skeletal muscle and promote a nitrate/nitrite reductive pathway for NO production to compensate for the loss of nNOS in skeletal muscle.

Methods:

Wild-type (WT) and genetic nNOS knockout (nNOS−/−) mice were fed normal chow (386.9 nmol/g nitrate) and subjected to three treatments: high-nitrate water (1 g/L sodium nitrate for 7 days), low-nitrate diet (46.8 nmol/g nitrate for 7 days), and low-nitrate diet followed by high-nitrate water for 7 days each.

Results:

High-nitrate water supplementation exhibited a greater and more significant increase in nitrate levels in skeletal muscle and blood in nNOS−/− mice than in WT mice. A low-nitrate diet decreased blood nitrate and nitrite levels in both WT and nNOS−/− mice. WT and nNOS−/− mice, treated with low-nitrate diet, followed by high-nitrate water supplementation, showed a significant increase in nitrate levels in skeletal muscle and blood, analogous to the increases observed in nNOS−/− mice supplemented with high-nitrate water. In skeletal muscle of nNOS−/− mice on high-nitrate water supplementation, on low-nitrate diet, and in low–high nitrate treatment, the loss of nNOS resulted in a corresponding increase in the expression of nitrate/nitrite reductive pathway-associated nitrate transporters [sialin and chloride channel 1 (CLC1)] and nitrate/nitrite reductase [xanthine oxidoreductase (XOR)] but did not show a compensatory increase in iNOS or eNOS protein and eNOS activation activity [p-eNOS (Ser1177)].

Discussion:

These findings suggest that a greater increase in nitrate levels in skeletal muscle of nNOS−/− mice on nitrate supplementation results from reductive processes to increase NO production with the loss of nNOS in skeletal muscle.
The effects of functional training on physical fitness and skill-related performance among basketball players: a systematic review
Authors: Cao Shudian , Liu Jia , Wang Zhaoran , Geok Soh Kim
Published: May 2024

Background: Evidence suggests that functional training (FT) positively impacts physical fitness and sports performance. However, a systematic review addressing the effects of FT on basketball players remains absent. This systematic review aims to explore the influence of FT on physical fitness and skill-related performance in basketball players.

Methods: We searched six databases: Web of Science, Scopus, PubMed, China National Knowledge Infrastructure (CNKI), EBSCOhost, and Google Scholar. The search utilized a combination of keywords related to FT, physical fitness, and basketball. The Eligibility Criteria of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) guidelines were followed in this systematic review.

Results: 11 studies were ultimately included in this review, collectively recruiting 333 basketball players. These studies demonstrated that FT significantly improved muscle strength, linear speed, cardiovascular endurance, flexibility, balance, and muscular endurance. However, the effects of FT on power, change-of-direction speed, and basketball-related performance were inconsistent. Most studies showed FT significantly improves these three variables, but a small number of studies did not find positive effects of FT using specific tests including standing long jump, Sargent jump, touch high, lane agility, lateral shuffle, dribbling line drill, and free-throw tests.

Conclusion: FT is an effective training method for enhancing physical fitness including muscle strength, linear speed, cardiovascular endurance, flexibility, balance, and muscular endurance. However, the effects of FT on power, change-of-direction speed, and basketball-related performance were divergent. Some tests were not improved after FT potentially due to the short program lengths and training session durations, varied athletic levels of players examined, and different foci of the FT exercises administered. The collective evidence suggests FT programs, especially the specific exercises prescribed, should be tailored to the desired training objectives. More studies investigating the effects of FT on physical fitness and basketball-related performance with established tests are encouraged in the future to expand the current evidence base.

Systematic Review Registration:https://inplasy.com/, Identifier INPLASY202360072.
The role of long-term target and masker talker familiarity in children’s speech-in-speech recognition
Authors: Flaherty Mary M.
Published: May 2024

Objectives

This study investigated the influence of long-term talker familiarity on speech-in-speech recognition in school-age children, with a specific emphasis on the role of familiarity with the mother’s voice as either the target or masker speech.

Design

Open-set sentence recognition was measured adaptively in a two-talker masker. Target and masker sentences were recorded by the adult mothers of the child participants. Each child heard sentences spoken by three adult female voices during testing; their own mother’s voice (familiar voice) and two unfamiliar adult female voices.

Study sample

Twenty-four school age children (8–13 years) with normal hearing.

Results

When the target speech was spoken by a familiar talker (the mother), speech recognition was significantly better compared to when the target was unfamiliar. When the masker was spoken by the familiar talker, there was no difference in performance relative to the unfamiliar masker condition. Across all conditions, younger children required a more favorable signal-to-noise ratio than older children.

Conclusion

Implicit long-term familiarity with a talker consistently improves children’s speech-in-speech recognition across the age range tested, specifically when the target talker is familiar. However, performance remains unaffected by masker talker familiarity. Additionally, while target familiarity is advantageous, it does not entirely eliminate children’s increased susceptibility to competing speech.
Empowering pro-environmental behavior in tourists through digital media: the influence of eco-guilt and empathy with nature
Authors: Chen Xin , Cheng Zhen-feng , Yang Hui-juan
Published: May 2024

In the digital economy era, leveraging digital media to foster tourists’ pro-environmental behavioral intention (TPEBI) has become crucial in the field of sustainable tourism. While existing studies have mainly focused on the driving mechanism of TPEBI within physical tourism contexts, the correlation between digital media information sharing and TPEBI remains unclear. Our study employs the cognitive-affective-conative framework to construct a theoretical model, considering eco-guilt and empathy with nature as mediating variables. It aims to explore the influencing mechanism of destination environmental information sharing through digital media on TPEBI from a presence perspective. Thereby, two scenario experiments were designed: Study 1 examined the impact of different formats of destination environmental threat information presentation on digital media on the sense of presence, while Study 2 explored the influencing mechanism of presence on TPEBI based on the conclusions of Study 1. Results indicate that (1) vivid and visible presentation formats of destination environmental threat information on digital media enhance individuals’ sense of presence; (2) sense of presence positively influences TPEBI; and (3) eco-guilt and empathy with nature mediate between presence and TPEBI. These findings not only contribute to theoretical and empirical research on digital media information sharing in sustainable tourism but also offer guidance for governments and tourism destinations to effectively stimulate TPEBI through digital media, achieve the sustainable development of destinations.
Increasing perceived health and mental health literacy among separated refugee Ukrainian families with urgent needs occasioned by invasion—a group intervention study with participatory methodology in Sweden
Authors: Ekblad Solvig , Gramatik Oksana , Suprun Yuliia
Published: May 2024

Background

With the increasing numbers of refugees from Ukraine affected by the ongoing war, there is a high risk of trauma-related stress due to low local health and mental health literacy care. Perceived good health is a human right. Earlier studies show that intervention for refugees can reduce and prevent post-migration stress and anxiety. The present explorative study aimed to investigate the feasibility and effectiveness of a short, trauma-focused group intervention (in Swedish “hälsoskola”) for Ukrainian-speaking refugees (EU’s mass refugee directive). This intervention was part of an ESF project aiming to get the subjects closer to the labor market in Västernorrland County, Sweden.

Materials and methods

A mixed-methods design, a participatory methodology, and an evaluation were used. Data were obtained with a short questionnaire in Ukrainian. It included a visual analogue health-rating scale, an observation, and an oral evaluation in groups. For practical and ethical reasons, there was no control group. Each group met five times for 2 h, a total of 10 h excluding pre- and post-assessment. There were eight sets of five group sessions per set, a total of 40 sessions. Brief initial and concluding breathing exercises sought to reduce stress among the participants.

Results

Baseline data were obtained from 99 participants, mostly females. Data gathered directly after the group intervention from 57 participants who had filled in both the pre- and post-questionnaires showed that (1) perceived anxiety/stress was significantly reduced (chi-2 25,53, df = 4, p < 001). (2) The participants showed significantly better perceived health as assessed on a visual analogue scale (average change from 63.16 to 71.18, p < 0.001). This result was supported by the participants’ questions, which were qualitatively evaluated using thematic content analysis. Five general themes stemmed from questions raised in dialogue with the participants plus observation with the respective local expert. The participants received answers to their questions, and their perceived negative attitudes to authorities changed to more positive ones.

Conclusion

By dialogue between the participants’ needs of knowledge and direct answers by the local expert, respectively, was feasible and effective as they perceived trust and health and mental health literacy increased. Implications for primary prevention are discussed.
Cross-shift changes in pulmonary function and occupational exposure to particulate matter among e-waste workers in Ghana
Authors: Laskaris Zoey , O'Neill Marie S. , Batterman Stuart A. , Mukherjee Bhramar , Fobil Julius N. , Robins Thomas G.
Published: May 2024

Introduction

Little is known on the association between cross-shift changes in pulmonary function and personal inhalation exposure to particulate matter (PM) among informal electronic-waste (e-waste) recovery workers who have substantial occupational exposure to airborne pollutants from burning e-waste.

Methods

Using a cross-shift design, pre- and post-shift pulmonary function assessments and accompanying personal inhalation exposure to PM (sizes <1, <2.5 μm, and the coarse fraction, 2.5–10 μm in aerodynamic diameter) were measured among e-waste workers (n = 142) at the Agbogbloshie e-waste site and a comparison population (n = 65) in Accra, Ghana during 2017 and 2018. Linear mixed models estimated associations between percent changes in pulmonary function and personal PM.

Results

Declines in forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) per hour were not significantly associated with increases in PM (all sizes) among either study population, despite breathing zone concentrations of PM (all sizes) that exceeded health-based guidelines in both populations. E-waste workers who worked “yesterday” did, however, have larger cross-shift declines in FVC [−2.4% (95%CI: −4.04%, −0.81%)] in comparison to those who did not work “yesterday,” suggesting a possible role of cumulative exposure.

Discussion

Overall, short-term respiratory-related health effects related to PM exposure among e-waste workers were not seen in this sample. Selection bias due to the “healthy worker” effect, short shift duration, and inability to capture a true “pre-shift” pulmonary function test among workers who live at the worksite may explain results and suggest the need to adapt cross-shift studies for informal settings.
The burden and treatment of non-communicable diseases among healthcare workers in sub-Saharan Africa: a multi-country cross-sectional study
Authors: Müller Sophie Alice , Elimian Kelly , Rafamatanantsoa Jean Florent , Reichert Felix , Mosala Francis , Böff Lena , Touré Sounan Fidèle , Boone Idesbald , Ravaoarisoa Lantonirina , Nduenga Sagesse , Ortu Giuseppina , Pozo-Martin Francisco , Tomczyk Sara , Eckmanns Tim , Okwor Tochi , Akoua-Koffi Chantal , Diané Bamourou , Randriamanantany Zely Arivelo , Ahuka Steve , Ochu Chinwe Lucia , El-Bcheraoui Charbel
Published: May 2024

Introduction

Non-communicable diseases (NCDs), the leading cause of death globally, are estimated to overtake communicable diseases in sub-Sahara Africa, where healthcare workers (HCWs) play a crucial role in prevention and treatment, but are in extreme shortage, thereby increasing the burden of NCDs among this specific population. To provide evidence for policy-making, we assessed the NCD burden, associated factors and treatment among HCWs in four sub-Saharan African countries.

Materials and methods

We conducted a cross-sectional study across four sub-Saharan African countries [Côte d'Ivoire (CIV), Democratic Republic of the Congo (DRC), Madagascar (MDG), and Nigeria (NIG)] between February and December 2022. In a standardized questionnaire, sociodemographic, chronic disease and treatment data were self-reported. We estimated the prevalence of (1) at least one chronic disease, (2) hypertension, and used backward elimination logistic regression model to identify risk factors.

Results

We recruited a total of 6,848 HCWs. The prevalence of at least one chronic disease ranged between 9.7% in NIG and 20.6% in MDG, the prevalence of hypertension between 5.4% in CIV and 11.3% in MDG. At most, reported treatment rates reached 36.5%. The odds of each of both outcomes increased with age (at least one chronic disease adjusted odds ratio: CIV: 1.04; DRC: 1.09; MDG: 1.06; NIG: 1.10; hypertension: CIV: 1.10; DRC: 1.31; MDG: 1.11; NIG: 1.11) and with BMI (at least one chronic disease: CIV: 1.10; DRC: 1.07; MDG: 1.06; NIG: 1.08; hypertension: CIV: 1.10; DRC: 1.66; MDG: 1.13; NIG: 1.07). Odds of both outcomes were lower among males, except in CIV. In NIG, the odds of both outcomes were higher among medical doctors and odds of hypertension were higher among those working in secondary care. In MDG, working in secondary care increased and working as auxiliary staff decreased the odds of at least one chronic disease.

Conclusion

The prevalence of self-reported chronic disease varied across the four sub-Saharan countries with potentially very low treatment rates. We identified several individual (age, sex, and BMI) and occupational (profession, level of healthcare) factors that influence the odds of NCDs. These factors should be taken into account when developing interventions addressing the burden and management of NCDs among HCWs.
Causality between sleep traits and the risk of frailty: a Mendelian randomization study
Authors: Deng Zhen , Hu Yifan , Duan Lincheng , Buyang Ziding , Huang Qian , Fu Xuedan , Luo Hong , Hou Tianshu
Published: May 2024

Background

Research based on observation has demonstrated a relationship between sleep traits and frailty; however, it remains uncertain if this correlation indicates causation. The purpose of this study was to look at the causal relationship that exists between frailty and sleep traits.

Method

Using summaries from a genome-wide association study of self-reported sleep features and frailty index, we performed a bidirectional Mendelian randomization (MR) analysis. Examining the causal relationships between seven sleep-related traits and frailty was the goal. The major method used to calculate effect estimates was the inverse-variance weighted method, supplemented by the weighted median and MR-Egger approaches. The study investigated pleiotropy and heterogeneity using several methodologies, such as the MR-Egger intercept, the MR-PRESSO approach, and the Cochran’s Q test. We took multivariate Mendelian randomization and genetic correlations between related traits to enhance the confidence of the results. Furthermore, we used MRlap to correct for any estimation bias due to sample overlap.

Results

Insomnia, napping during the day, and sleep apnea syndrome exhibited a positive connection with the frailty index in forward MR analysis. Conversely, there is a negative link between getting up in the morning, snoring and sleep duration with the frailty index. During the reverse MR analysis, the frailty index exhibited a positive correlation with insomnia, napping during the day, and sleep apnea syndrome, while demonstrating a negative correlation with sleep duration. There was no direct correlation between snoring, chronotype, and frailty. In MVMR analyses, the causal effect of sleep characteristics on frailty indices remained consistent after adjusting for potential confounders including BMI, smoking, and triglycerides.

Conclusion

The findings of our investigation yield novel evidence that substantiates the notion of a bidirectional causal connection between sleep traits and frailty. Through the optimization of sleep, it is potentially feasible to hinder, postpone, or even reverse the state of frailty, and we proposed relevant interventions.
Sports preferences in children and adolescents in psychiatric care—evaluation of a new questionnaire
Authors: Breido Florian , Stumm Sebastian , Jenetzky Ekkehart , Huss Michael
Published: May 2024

Introduction

As part of an exploratory and hypothesis-generating study, we developed the Sports Preference Questionnaire (SPOQ) to survey the athletic behavior of mentally ill children and adolescents, subjectively assessed physical fitness and perceived psychological effects of physical activity.

Methods

In a department of child and adolescent psychiatry, we classified 313 patients (6–18 years) according to their primary psychiatric diagnosis. The patients or—in the parental version of the questionnaire—their parents reported their sport preferences on the SPOQ. As possibly influential factors, we also assessed the frequency of physical activity, the importance of a trainer, coping with everyday life through physical activity, and subjectively perceived physical fitness.

Results

One in 3 patients (32.4%) stated that they were not physically active. Patients diagnosed with eating disorders reported, on average, a notably high frequency (median of 3 h/week) and degree of coping with daily life through physical activity (median of 5 on a 6-point Likert scale). Patients with anxiety disorders and depression had the lowest self-perception of physical fitness (mean value of 3.1 or 3.7 on an interval scala from 0 to 9). The presence of a trainer was generally considered not important, except for ADHD patients (median of 3 on a 6-point Likert scale).

Conclusion

The SPOQ is sensitive for differential effects of core child and adolescent disorders as well as for main covariates influencing the complex association between physical activity and emotional and behavioral disorders in children and adolescents. Based on this pilot study, we discussed the need for an efficacy study to measure the effects of sports therapy.
Advanced and sustainable manufacturing methods of polymer-based membranes for gas separation: a review
Authors: Alkandari Sharifah H. , Castro-Dominguez Bernardo
Published: May 2024

The fabrication of membranes for gas separation presents challenges that hinder their deployment as a truly sustainable technology. This review systematically explores the evolution and advancements in materials and manufacturing methods of polymer-based membranes, with a keen emphasis on sustainability and efficiency. The review delineates a broad spectrum of manufacturing techniques, ranging from traditional methods to cutting-edge approaches such as layer-by-layer assembly, and green synthesis, highlighting their implications for environmental sustainability, performance enhancement, scalability, and economic viability. Key findings indicate a significant shift towards greener solvents, bio-based polymers and processes that reduce waste and costs. Critical analysis uncovers a growing focus on understanding the life cycle of membranes and developing strategies for end-of-life such as recycling and the use of biodegradable materials, underscoring the commitment of the community to minimizing environmental footprints.
Design and evaluation of an immunology and pathology course that is tailored to today’s dentistry students
Authors: de Vries Teun J. , Schoenmaker Ton , Peferoen Laura A. N. , Krom Bastiaan P. , Bloemena Elisabeth
Published: May 2024

Curricular reform provides new opportunities to renovate important pillars of the dentistry curriculum, such as immunology and pathology, with novel approaches that appeal to new generations of students. When redesigning a course that integrates both immunology and pathology at the level that provides dentistry students with sustainable knowledge that is useful for their entire career, several challenges must be met. The objective of the present study was to describe the considerations involved in the design phase of such a new course. First, the course should be compatible with the new view on the incorporation of more active learning and teaching methods. Practically, this means that the course design should contain fewer lectures and more seminars and tutorials, where the students have fewer contact hours and actively engage in using recently acquired knowledge within a contextual background. A mandatory session of team-based learning provides opportunities to apply knowledge in combination with academic reasoning skills, teamwork, and communication. Second, for a 4-week course, choices must be made: students will not become immunologists nor pathologists in such a short period. A governing principle for this course's design is that it should be based on understanding the basic principles of immunology and pathology. The ultimate goal for the students is to make the course immuno-logical and patho-logical, challenging them to reach a next level but clearly without oversimplification. Part of the course design should allow room for students to further study an immunological topic of their own choice, thereby contributing to their immunological curiosity and to their academic development. Third, to make it tailored to a new generation of dentists, examples from the field of dentistry are actively integrated in all aspects of the course. Finally, the era of ChatGPT provides novel opportunities to use generative artificial intelligence (AI) tools in the learning process, but it demands critical thinking of how to use it in a newly designed course. A mid-course evaluation revealed that students acknowledged that immunology and pathology were presented as an integrated course. The final course evaluation endorsed the use of these various educational methods. These methods proved to be appropriate and logical choices for reaching the learning goals of the course.
Animal models to study the pathogenesis and novel therapeutics of oral lichen planus
Authors: Choi Youngnim
Published: May 2024

Oral lichen planus (OLP) is a prevalent oral mucosal disease characterized by an unknown etiology and a complex pathogenesis. Patients with OLP endure a chronic course marked by alternating non-erosive and erosive lesions, with no definitive cure currently available. Particularly challenging is the treatment of recalcitrant erosive OLP, highlighting an urgent need for therapies targeting specific pathogenic pathways. In diseases like OLP, where the etiopathogenesis is intricate and elusive, animal models are indispensable for hypothesis testing and elucidating disease mechanisms. To date, only three animal models for oral lichenoid lesions have been reported in the literature. This Perspective paper evaluates these existing models, along with a novel OLP mouse model introduced at the 3rd International Conference on Oral Mucosal Immunity and Microbiome. The validity of these models is critically assessed, and their potential future applications in advancing our understanding of OLP are discussed.
“He’ll come with some sugar.” A qualitative study exploring the drivers and consequences of schoolgirls transactional sex behaviours
Authors: Thiaw Yandé , Nyothach Elizabeth , Zulaika Garazi , van Eijk Anna Maria , Fwaya Eunice , Obor David , Phillips-Howard Penelope , Mason Linda
Published: May 2024

Intoduction

Transactional sex (TS) is common in areas of sub-Saharan Africa, motivated by reasons beyond financial support. Through this qualitative study we sought to understand the motivation driving TS among adolescent schoolgirls in rural western Kenya where rates are reportedly high. Identifying and understanding drivers within the local context is necessary for implementation of successful public health policy and programming to reduce the associated harms impacting health and wellbeing.

Methods

To understand the drivers of sexual behaviors, individual views, and socio-cultural norms, we spoke with schoolgirls, male peers, parents and teachers. The three latter groups may influence, encourage, and shape girls' views and behaviors and thus contribute to the perpetuation of cultural and societal norms.

Results

One hundred and ninety-nine participants took part across 20 FGDs; 8 comprised of schoolgirl groups, and 4 each of schoolboy, parent or teacher groups. Through thematic analysis, poverty emerged as the key driver of TS and a normative behaviour amongst secondary school girls. Subthemes including parental influence, need for menstrual pads, pressure from boda boda drivers, peer pressure, and blame were part of a complex relationship linking poverty with TS.

Discussion

We conclude that whilst TS is perceived as inevitable, normal and acceptable it is not really a choice for many girls. Exploring ways to encourage communication between families, including around menstruation, may help enable girls to ask for help in acquiring essential items. In addition, education at a community level may shift social norms over time and decrease the prevalence of age-disparate TS among schoolgirls and older, wealthier men in the community.
Reinforcement learning for watershed and aquifer management: a nationwide view in the country of Mexico with emphasis in Baja California Sur
Authors: Ortega Roberto , Carciumaru Dana , Cazares-Moreno Alexandra D.
Published: May 2024

Reinforcement Learning (RL) is a method that teaches agents to make informed decisions in diverse environments through trial and error, aiming to maximize a reward function and discover the optimal Q-learning function for decision-making. In this study, we apply RL to a rule-based water management simulation, utilizing a deep learning approach for the Q-learning value function. The trained RL model can learn from the environment and make real-time decisions. Our approach offers an unbiased method for analyzing complex watershed scenarios, providing a reward function as an analytical metric while optimizing decision-making time. Overall, this work underscores RL’s potential in addressing complex problems, demanding exploration, sequential decision-making, and continuous learning. External variables such as policy shifts, which are not readily integrated into the model, can substantially influence outcomes. Upon establishing a model with the requisite minimal states and actions, the subsequent learning process is relatively straightforward, depending on the selection of appropriate RL model algorithms. Its application depends on the specific problem. The primary challenge in this modeling approach lies in model definition, specifically in devising agents and actions that apply to complex scenarios. Our specific example was designed to address recent decision-making challenges related to constructing dams due to water scarcity. We present two examples: one from a nationwide perspective in Mexico and the other focused on Baja California Sur, the state with the highest water stress. Our results demonstrate our capability to prioritize watersheds effectively for the most significant benefits, particularly dam construction.
Ice Storms: Not as Cool as They Seem
Authors: Rustad Lindsey , Leuenberger Wendy
Published: May 2024

Ice storms might sound fascinating, but for forests, they are not as cool as they seem! Ice storms happen when freezing rain coats trees, branches, and the forest floor in a shiny, frozen glaze. These storms can be a big deal because they can affect our forests and the things we care about, like, wood, wildlife, and places for recreation. Despite this, we do not know very much about how ice storms affect forests. Scientists at the Hubbard Brook Experimental Forest, New Hampshire (USA), decided to learn more. They used fire-fighting pumps and hoses to create experimental ice storms in the forest during the winters of 2016 and 2017. They found that these experimental storms caused a lot of short- and long-term damage to trees, including breaking of twigs and branches. In a nutshell, ice storms are not just about frozen beauty—they have real consequences for our forests and species that are not used to ice!
Transport of energetic particles in turbulent space plasmas: pitch-angle scattering, telegraph, and diffusion equations
Authors: Shalchi Andreas
Published: May 2024

Introduction: In this article, we revisit the pitch-angle scattering equation describing the propagation of energetic particles through magnetized plasma. In this case, solar energetic particles and cosmic rays interact with magnetohydrodynamic turbulence and experience stochastic changes in the pitch-angle. Since this happens over an extended period of time, a pitch-angle isotropization process occurs, leading to parallel spatial diffusion. This process is described well by the pitch-angle scattering equation. However, the latter equation is difficult to solve analytically even when considering special cases for the scattering coefficient.

Methods: In the past, a so-called subspace approximation was proposed, which has important applications in the theory of perpendicular diffusion. Alternatively, an approach based on the telegraph equation (also known as telegrapher’s equation) has been developed. We show that two-dimensional subspace approximation and the description based on the telegraph equation are equivalent. However, it is also shown that the obtained distribution functions contain artifacts and inaccuracies that cannot be found in the numerical solution to the problem. Therefore, an N-dimensional subspace approximation is proposed corresponding to a semi-analytical/semi-numerical approach. This is a useful alternative compared to standard numerical solvers.

Results and Discussion: Depending on the application, the N-dimensional subspace approximation can be orders of magnitude faster. Furthermore, the method can easily be modified so that it can be used for any pitch-angle scattering equation.
The effect of a 10-week TOCA Football System intervention program on sport-specific motor skills among junior footballers
Authors: Szabó Zoltán Tamás , Derkács Evelin , Deli Balázs , Prémusz Viktória , Vass Lívia , Pusztafalvi Henriette , Ács Pongrác
Published: May 2024

Introduction

The objective of our study was to examine, in addition to using the TOCA Football System tool and training method, the effect of a 10-week intervention on elite youth athletes in terms of their sport-specific motor skills and anthropometric variables.

Methods

The study covered a group of 32 young players practicing football (U14) (13.45 ± 0.64 years). The junior U14 footballers were randomly assigned to an intervention or TOCA group (TG, N = 15, 13.25 ± 0.58 years) and a control group (CG, N = 17, 13.63 ± 0.66 years). Before starting the test, we performed full anthropometric measurements and assessed the sample's agility with and without the ball and their sport-specific endurance. The measurements were then repeated after the 10-week intervention.

Results

Within-group analysis showed significant improvements in muscle mass (p < 0.001), sport-specific endurance (p < 0.001), (p < 0.004) and agility (in TG) both with and without the ball (p = 0.002), (p = 0.004) however, we did not find a significant change in body fat percentage in either group (p = 0.988, p = 0.288). In the CG, “agility with the ball” changed significantly only (p = 0.023). In the between-group analysis with a repeated-measures analysis of variance (mixed-design ANOVA), there was no significant interaction in any performance variables. The main findings of this study indicate that a TOCA Football training program in addition to normal training during the in-season period does not produce additional effects in anthropometric factors, sport-specific endurance and agility performance with the ball (dribbling) and without the ball in comparison with the control condition.

Discussion

From a practical point of view, the presented anthropometric and physical profiles of players can be useful for football coaches in optimizing soccer training. Overall, it also can be concluded that the device can be safely used in the sensitive age group in terms of the development of motor skills since we did not find any negative effects during the use of the device in terms of the parameters we examined. In addition to the expansion of the number of elements and the inclusion of other age groups, it is advisable to carry out further complex tests, as the TOCA Football System offers many research opportunities.
Assessing concordance between Campylobacter prevalence in broilers and human cases before and during the COVID-19 pandemic in Lower Saxony, Germany, considering fresh chicken meat consumption patterns
Authors: Nolte Tobias , Spieß Fabian , Jacobs Anne-Katrin , Kemper Nicole , Visscher Christian
Published: May 2024

As the most common foodborne disease, number of campylobacteriosis decreased in Germany with the beginning of the COVID-19 pandemic in 2020. As the consumption of fresh chicken meat is a major risk factor for human infection, this study investigated the relationship between Campylobacter contamination levels on chicken carcasses and human cases in Lower Saxony, Germany and observed fresh chicken meat consumption patterns between 2018 and 2021 including the time of the COVID-19 pandemic. Campylobacter levels in broilers and human cases were classified based on the median and descriptively analysed per week using contingency tables. Before the COVID-19 pandemic (2018 and 2019), high Campylobacter contamination levels on neck samples and many human cases were more present, whereas with the beginning of the COVID-19 pandemic (2020 and 2021), low contamination levels on chicken carcasses and few human cases were more present. Lowest concordance between both parameters was shown in 2018 (Cohen’s cappa coefficient: 0.37) and 2020 (0.38). The highest concordance was examined in 2021 (0.69). The private consumption of fresh chicken meat in Lower Saxony increased significantly with the beginning of the COVID-19 pandemic in 2020 by 63.9 tonnes compared to 2019 to an average of 453.5 tonnes per week. Public health measures and a reduced number of medical treatments have undoubtedly had an impact on less reported human cases during the COVID-19 pandemic. However, number of human cases remained at a low level in Germany in 2023 while chicken meat consumption increased. Thus, further risk assessments regarding the risk of campyloabcteriosis due to chicken meat consumption should include the country of origin, as the level of contamination of chicken carcasses varies between European countries.
CT reconstruction based 3D model of the digital cushion’s blood supply in the hind foot of an African savanna elephant (Loxodonta africana)
Authors: Reinitz László Zoltán , Lenzing Franka , Papp Endre , Biácsi Alexandra , Fajtai Dániel , Petneházy Örs
Published: May 2024

Introduction

Foot health is crucial for elephants, as pathological lesions of the feet are a leading cause of euthanasia in captive elephants, which are endangered species. Proper treatment of the feet, particularly in conditions affecting the digits and the digital cushion, requires a thorough understanding of the underlying anatomy. However, only limited literature exists due to the small population and the epidemiological foot diseases which often precludes many deceased elephants from scientific study. The aim of this study was to provide a detailed anatomical description of the blood supply to the African elephant’s hindfoot.

Methods

The healthy right hindlimb of a 19-year-old deceased female African savanna elephant was examined using computed tomography. Following a native sequence, 48 mL of barium-based contrast agent was injected into the caudal and cranial tibial arteries, and a subsequent scan was performed. The images were processed with 3D Slicer software.

Results

The medial and lateral plantar arteries run in a symmetrical pattern. They each have a dorsal and a plantar branch, which reach the plantar skin before turning toward the axial plane of the sole to reach the digital cushion from the proximal direction. An accurate 3D model of the arteries and the bones of the foot, a set of labeled images and an animation of the blood supply have been created for ease of understanding.

Discussion

In contrast to domestic ungulates, the digital cushion of the hindlimb is supplied differently from that of the forelimb. The lack of large vessels in its deeper layers indicates a slow regeneration time. This novel anatomical information may be useful in the planning of surgical interventions and in emergency medical procedures.
Mechanism of Evolution of Shock Wave of Muzzle Jet under Initial Interference and Its Simplified Model
Authors: Li Zijie , Wang Hao
Published: May 2024

Large-caliber and long-barrel weapons are important experimental devices for exploring the impact resistance and reliability of warheads. The force of impact of the muzzle jet has a significant influence on the overload resistance of the warhead and surrounding devices. The mechanism of motion of the body inside the tube cannot be ignored owing to the high kinetic energy at the muzzle. In this study, we designed the relevant experiment and a simulation model to analyze the structural characteristics and mechanism of evolution of the shock wave and the vortex structure in a muzzle jet. The aim was to examine the evolution of the shock wave with initial jet-induced interference. And we established three other simulation models to compare the similarities and differences between the results of the models. The results showed that, in the original complex model, the initial jet restricted the free expansion of the muzzle jet, and this led to many shock–shock collisions that retarded the development of the shock waves. Multiple reflected shock waves were thus formed under a high local pressure that distorted the shock structure, while the structure of the shock wave in the simplified models was clear and simple. The parameters of motion of the body changed by a little when the initial jet-induced interference was ignored. The difference in values of the strongest impact force measured at monitoring points far from the muzzle was small, with an error of about 2%, such that the simplified model without the initial jet could be used in place of the original complex model. The other simplified models yielded significant differences. Our results provide an important theoretical basis for further research on the muzzle jet and its applications in engineering.
Energy Compensation for Crop Growth under Plastic Mulching: Theories, Models, and Limitations
Authors: Ding Dianyuan , Li Ting , Wu Lihong , Zhang Xi , Zhao Ying , Feng Hao , Zhang Chao , Wendroth Ole
Published: May 2024

Plastic film mulching (PM) is a useful agronomic means to adapt to the environmental conditions of dryland agriculture and improve crop production. To improve the theoretical framework of PM technology, this study focuses on the interaction between the soil temperature change caused by PM and crop growth. The definition, action mechanism, and simulation of the compensatory effect of PM on growing degree days are introduced to reveal the effect of soil temperature under PM on crop development and growth. Our summary shows that the strength of the warming effect changes with the growth and development of crops, strengthening during the early stage of crop growth and gradually weakening as a crop canopy develops. Generally, the warming effect has a good promotion effect on crop growth, but the crop growth is hampered even with a yield reduction when the increased soil temperature caused by PM exceeds the tolerant temperature for plant growth. Moreover, the compensatory effect of PM could be used to quantify the growth and development of crops under PM and has been widely applied to cotton, corn, winter wheat, and rice. The compensation coefficient is larger in the early stage of crop growth than in the later stage. The compensation coefficient has certain differences for the same crop because of the influence of climate factors, soil moisture content, and soil microtopography. In future research, the theoretical integration of the safety period of PM and the time threshold of the compensatory effect could be theoretically interpreted, and the construction of the compensatory effect module in the crop models will also be an important issue.
Identification of Copy Number Variations and Selection Signatures in Wannan Spotted Pigs by Whole Genome Sequencing Data: A Preliminary Study
Authors: Zhang Wei , Jiang Yao , Ni Zelan , Zhou Mei , Liu Linqing , Li Xiaoyu , Su Shiguang , Wang Chonglong
Published: May 2024

Copy number variation (CNV) is an important structural variation used to elucidate complex economic traits. In this study, we sequenced 25 Wannan spotted pigs (WSPs) to detect their CNVs and identify their selection signatures compared with those of 10 Asian wild boars. A total of 14,161 CNVs were detected in the WSPs, accounting for 0.72% of the porcine genome. The fixation index (Fst) was used to identify the selection signatures, and 195 CNVs with the top 1% of the Fst value were selected. Eighty genes were identified in the selected CNV regions. Functional GO and KEGG analyses revealed that the genes within these selected CNVs are associated with key traits such as reproduction (GAL3ST1 and SETD2), fatty acid composition (PRKG1, ACACA, ACSL3, UGT8), immune system (LYZ), ear size (WIF1), and feed efficiency (VIPR2). The findings of this study contribute novel insights into the genetic CNVs underlying WSP characteristics and provide essential information for the protection and utilization of WSP populations.
Lactic Acid Bacteria and Bacillus subtilis as Potential Protective Cultures for Biopreservation in the Food Industry
Authors: Garin-Murguialday Nerea , Espina Laura , Virto Raquel , Pagán Rafael
Published: May 2024

The use of bacteria and/or their compounds is an alternative to the use of positive-list additives that the food industry is using as a tool to meet consumer demands for more natural, long-shelf-life, and healthy products, in short, to offer clean label foods. The aim of this study is to investigate the suitability of cell-free supernatants (CFSs) from Qualified Presumption of Safety strains as bioprotective cultures. Out of an initial screening panel of about 200 isolates, strains Pediococcus acidilactici CNTA 1059, Lactiplantibacillus plantarum CNTA 600, Levilactobacillus brevis CNTA 1374, and Bacillus subtilis CNTA 517 demonstrated strong antimicrobial activity against, especially, Gram-positive bacteria. The CFSs of these four strains showed minimum inhibitory concentration values between 0.15% and 5% against Listeria monocytogenes and Lentilactobacillus parabuchneri. None of the four selected strains exhibited acquired resistance to target antibiotics, and the non-toxigenicity of all the CFSs was demonstrated. In the case of the three lactic acid bacteria, the presence of bacteriocin-like inhibitory substances was confirmed following the decline in antimicrobial activity due to treatment with proteases. Regarding B. subtilis, biosynthetic gene clusters for different bacteriocin-like substances, including protease-resistant lipoproteins, were found via whole-genome sequencing. In addition, all of the CFSs exhibited stable antimicrobial activity at a wide range of temperatures (70–121 °C) used for the pasteurization and sterilization of food products, with a loss of antimicrobial activity ranging from 3% to 28%. These results point to the possibility that CFSs from these strains could be used in the food industry as a biocontrol tool to develop new products.
Enhancing Botnet Detection in Network Security Using Profile Hidden Markov Models
Authors: Mannikar Rucha , Di Troia Fabio
Published: May 2024

A botnet is a network of compromised computer systems, or bots, remotely controlled by an attacker through bot controllers. This covert network poses a threat through large-scale cyber attacks, including phishing, distributed denial of service (DDoS), data theft, and server crashes. Botnets often camouflage their activity by utilizing common internet protocols, such as HTTP and IRC, making their detection challenging. This paper addresses this threat by proposing a method to identify botnets based on distinctive communication patterns between command and control servers and bots. Recognizable traits in botnet behavior, such as coordinated attacks, heartbeat signals, and periodic command distribution, are analyzed. Probabilistic models, specifically Hidden Markov Models (HMMs) and Profile Hidden Markov Models (PHMMs), are employed to learn and identify these activity patterns in network traffic data. This work utilizes publicly available datasets containing a combination of botnet, normal, and background traffic to train and test these models. The comparative analysis reveals that both HMMs and PHMMs are effective in detecting botnets, with PHMMs exhibiting superior accuracy in botnet detection compared to HMMs.