bluuebunny/crossref_metadata_2025 huggingface dataset:
CFD-Based Simulation Analysis for Motions through Multiphase Environments
Authors: Wang Shuqi , Fan Jizhuang , Liu Yubin
Published: October 2023

The motion process and force of the jumper crossing a multiphase environment are of great significance to the research of small amphibious robots. Here, CFD (Computational Fluid Dynamics)-based simulation analysis for motions through multiphase environments (water–air multiphase) is successfully realized by UDF (user-defined function). The analytical model is first established to investigate the jumping response of the jumpers with respect to the jump angle, force, and water depth. The numerical model of the jumper and its surrounding fluid domain is conducted to obtain various dynamic parameters in the jumping process, such as jumping height and speed. Satisfactory agreements are obtained by comparing the error of repeated simulation results (5%). Meanwhile, the influence of the jumper’s own attributes, including mass and structural size, on the jumping performance is analyzed. The flow field information, such as wall shear and velocity when the jumper approaches and breaks through the water surface, is finally extracted, which lays a foundation for the structural design and dynamic underwater analysis of the amphibious robot.
An Update on the Role of MRI in Treatment Stratification of Patients with Cervical Cancer
Authors: Shakur Amreen , Lee Janice Yu Ji , Freeman Sue
Published: October 2023

Cervical cancer is the fourth most common cancer in women worldwide and the most common gynaecological malignancy. The FIGO staging system is the most commonly utilised classification system for cervical cancer worldwide. Prior to the most recent update in the FIGO staging in 2018, the staging was dependent upon clinical assessment alone. Concordance between the surgical and clinical FIGO staging decreases rapidly as the tumour becomes more advanced. MRI now plays a central role in patients diagnosed with cervical cancer and enables accurate staging, which is essential to determining the most appropriate treatment. MRI is the best imaging option for the assessment of tumour size, location, and parametrial and sidewall invasion. Notably, the presence of parametrial invasion precludes surgical options, and the patient will be triaged to chemoradiotherapy. As imaging is intrinsic to the new 2018 FIGO staging system, nodal metastases have been included within the classification as stage IIIC disease. The presence of lymph node metastases within the pelvis or abdomen is associated with a poorer prognosis, which previously could not be included in the staging classification as these could not be reliably detected on clinical examination. MRI findings corresponding to the 2018 revised FIGO staging of cervical cancers and their impact on treatment selection will be described.
Personalized Therapy of Sarcomas
Authors: Desar Ingrid M. E. , Rothermundt Christian
Published: October 2023

Sarcomas are a group of rare malignant mesenchymal tumors [...]
Comparative Analysis of the GNAI Family Genes in Glioblastoma through Transcriptomics and Single-Cell Technologies
Authors: Raza Ahmad , Yen Meng-Chi , Anuraga Gangga , Shahzadi Iram , Mazhar Muhammad Waqar , Ta Hoang Dang Khoa , Xuan Do Thi Minh , Dey Sanskriti , Kumar Sachin , Santoso Adrian Wangsawijaya , William Bianca Tobias , Wang Chih-Yang
Published: October 2023

Glioblastoma multiforme (GBM) is one of the most aggressive cancers with a low overall survival rate. The treatment of GBM is challenging due to the presence of the blood–brain barrier (BBB), which hinders drug delivery. Invasive procedures alone are not effective at completely removing such tumors. Hence, identifying the crucial pathways and biomarkers for the treatment of GBM is of prime importance. We conducted this study to identify the pathways associated with GBM. We used The Cancer Genome Atlas (TCGA) GBM genomic dataset to identify differentially expressed genes (DEGs). We investigated the prognostic values of the guanine nucleotide-binding protein G(i) alpha subunit (GNAI) family of genes in GBM using a Chinese Glioma Genome Atlas (CGGA) dataset. Within this dataset, we observed the association in the tumor microenvironment between the gene expression of GNAI subunit 3 (GNAI3) and a poor prognosis. MetaCore and gene ontology (GO) analyses were conducted to explore the role of GNAI3 in co-expressed genes and associated signaling pathways using a transcript analysis. Notable pathways included “Cytoskeleton remodeling regulation of actin cytoskeleton organization by the kinase effectors of Rho GTPases” and “Immune response B cell antigen receptor (BCR) pathway”. A single-cell analysis was used to assess GNAI3 expression in GBM. The results demonstrated that GNAI family genes, specifically GNAI3, were significantly associated with carcinogenesis and malignancy in GBM patients. Our findings suggest that the GNAI3 gene holds potential as a prognostic biomarker for GBM.
Electron-Beam Processing of Aluminum-Containing Ceramics in the Forevacuum Pressure Range
Authors: Klimov Aleksandr , Bakeev Ilya , Zenin Aleksey
Published: October 2023

Aluminum–ceramic materials based on Al2O3 and AlN are widely used in the electronics industry and, according to a number of electrophysical and technical and economic parameters, are among the most suitable for the production of electrical and radio engineering products. In this study, it is shown that the treatment of ceramics based on Al2O3 with an electron beam with a power of 200–1100 W and a current of 10–50 mA leads to heating of the ceramic surface to a temperature of 1700 °C. When heated to a temperature of 1500 °C and kept at this temperature for no more than 10 s, an increase in the roughness of the ceramic surface is observed by more than an order of magnitude. At the same time, for ceramic substrates based on aluminum nitride, an increase in the temperature of electron beam treatment from 1300 to 1700 °C leads to an increase in thermal conductivity from 1.5 to 2 times. The edge angle of water wetting of the AlN surface can vary from 20 to 100 degrees depending on the processing temperature, which allows one to control the transition of the material from a hydrophilic to a hydrophobic state. At the same time, electron beam exposure to Al2O3 does not change the wettability of this material so much. Electron beam processing in the forevacuum pressure region allows controlled changes in the electrophysical properties of ceramic materials based on Al2O3 and AlN.
Reducing Environmental Impacts at a Midwestern Academic Medical Center: Making Carbon Emissions Reduction a Reality
Authors: Bjorgaard Johannah , Potter Teddie
Published: October 2023

(1) Background: Human-generated greenhouse gas (GHG) emissions are the largest contributor to climate change worldwide. Climate change negatively impacts human and planetary health, threatening the existence of life on earth. The healthcare industry is responsible for approximately 8.5% of carbon emissions in the United States. Measuring baseline GHG emissions is the first step in emissions reduction. However, very few models of measurement exist for health care organizations. This project aimed to develop and implement a program to measure and track GHG emissions at a midwestern academic medical center (MAMC) and to educate staff on how to manage the process. (2) Methods: A Plan, Do, Study, Act (PDSA) cycle and Quality Improvement methodology were used to develop, implement, and assess a standardized GHG emission inventory process to measure Scope 1 and Scope 2 baseline emissions and provide virtual training and education to the accountable staff. A pre- and post-survey design was used to measure the knowledge and readiness of the staff after the implementation of the GHG inventory training. (3) Results: The GHG inventory process was validated through an external verification process, and the measurement of Scope 1 and Scope 2 baseline GHG emissions was completed and verified for accuracy through a data comparison review. The pre-post-training survey showed an increase in the knowledge and readiness of staff to maintain a GHG inventory. (4) Conclusions: This work shows the feasibility of obtaining baseline GHG emissions data at large medical centers. It represents the initial phase of the overarching goal to develop site-wide and system-wide carbon reduction strategies and a climate action plan within this health system.
The Sleep of Shift Workers in a UK Financial Organisation and Associations with Mental, Physical, Social and Cognitive Health
Authors: Illingworth Gaby , McMahon William R. , Gartland Dabhnait , Pereira Stephen , Foster Russell G.
Published: October 2023

Shift workers are vulnerable to circadian misalignment, sleep disturbance and increased risk of impaired health. Studies concerning the sleep and health of individuals working shifts in the financial sector are lacking. We investigated sleep quality, sleep duration and associations with health in a UK financial organisation. Employees (n = 178; 61% male) completed an online survey comprising the SSI, PSQI, GAD-7, PHQ-9, WAFCS, EMQ-R and BMI. Three-quarters of employees reported poor sleep quality. Poorer sleep quality and shorter sleep duration were both associated with greater anxiety and depression symptoms. However, sleep quality demonstrated greater explained variance with symptom severity (anxiety: Rs2 difference = 13.2%; depression: Rs2 difference = 21.8%). Poorer sleep quality was associated with higher BMI, greater work-to-family conflict and poorer everyday memory (rs = 0.26–0.29), while shorter sleep duration was only significantly associated with higher BMI. Sleep quality did not differ depending on the nightshift type (“permanent”/“other”). Sleep achieved was shorter than perceived sleep-need for all consecutive shift types—especially night shifts (40 min–1 h 24 min). This preliminary study suggests that sleep quality, and to a lesser extent sleep duration, are associated with a range of health outcomes for shift workers within the financial sector, highlighting the need to increase organisational awareness of the importance of sleep for employee health.
Monitoring the Population Development of Indicator Plants in High Nature Value Grassland Using Machine Learning and Drone Data
Authors: Gröschler Kim-Cedric , Muhuri Arnab , Roy Swalpa Kumar , Oppelt Natascha
Published: October 2023

The temporal monitoring of indicator plant species in high nature value grassland is crucial for nature conservation. However, traditional monitoring approaches are resource-intensive, straining limited funds and personnel. In this study, we demonstrate the capabilities of a repeated drone-based plant count for monitoring the population development of an indicator plant species (Dactylorhiza majalis (DM)) to address such challenges. We utilized multispectral very high-spatial-resolution drone data from two consecutive flowering seasons for exploiting a Random Forest- and a Neural Network-based remote sensing plant count (RSPC) approach. In comparison to in situ data, Random Forest-based RSPC achieved a better performance than Neural Network-based RSPC. We observed an R² of 0.8 and 0.63 and a RMSE of 8.5 and 11.4 DM individuals/m², respectively. The accuracies indicate a comparable performance to conventional plant count surveys. In a change detection setup, we assessed the population development of DM and observed an overall decline in DM individuals in the study site. Regions with an increasing DM count were small and the increase relatively low in magnitude. Additionally, we documented the success of a manual seed transfer of DM to a previously uninhabited area within our study site. We conclude that repeated drone surveys are indeed suitable to monitor the population development of indicator plant species with a spectrally prominent flower color. They provide a unique spatio-temporal perspective to aid practical nature conservation and document conservation efforts.
Detection of False Data Injection Attacks in a Smart Grid Based on WLS and an Adaptive Interpolation Extended Kalman Filter
Authors: Zhang Guoqing , Gao Wengen , Li Yunfei , Guo Xinxin , Hu Pengfei , Zhu Jiaming
Published: October 2023

An accurate power state is the basis of the normal functioning of the smart grid. However, false data injection attacks (FDIAs) take advantage of the vulnerability in the bad data detection mechanism of the power system to manipulate the process of state estimation. By attacking the measurements, then affecting the estimated state, FDIAs have become a serious hidden danger that affects the security and stable operation of the power system. To address the bad data detection vulnerability, in this paper, a false data attack detection method based on weighted least squares (WLS) and an adaptive interpolation extended Kalman filter (AIEKF) is proposed. On the basis of applying WLS and AIEKF, the Euclidean distance is used to calculate the deviation values of the two-state estimations to determine whether the current moment is subjected to a false data injection attack in the power system. Extensive experiments were conducted to simulate an IEEE-14-bus power system, showing that the adaptive interpolation extended Kalman filter can compensate for the deficiency in the bad data detection mechanism and successfully detect FDIAs.
Evaluation of the Optional Wideband Accuracy of Inductive Current Transformers in Accordance with the Standard IEC 61869-1 Ed.2
Authors: Stano Ernest , Kaczmarek Piotr , Kaczmarek Michal
Published: October 2023

This paper presents the evaluation of tested inductive CTs’ accuracy for distorted current harmonics in accordance with the optional accuracy class WB1 introduced by the new edition of the standard IEC 61869-1 published in the year 2023. The tests were performed in compliance with the interpretation sheet IEC 61869-2:2012/ISH1:2022. Therefore, the resistive and the resistive–inductive loads of the secondary winding of tested inductive CTs were used, as this was required for the given test conditions. The results indicate that the units designed for the transformation of a sinusoidal current of a frequency of 50 Hz ensure the high wideband transformation accuracy of the distorted current harmonics, as demanded by the power quality monitoring and distorted electrical power and energy requirements. The key to this is proper design using modern magnetic material(s) for the magnetic core and its oversizing in relation to the requirements for a given accuracy class defined for the transformation of sinusoidal currents with a rated frequency. Both tested inductive CTs with a rated primary current RMS value equal to 300 A, class 0.2 and 0.5, ensured compliance with the requirements of the WB1 wideband accuracy class.
Theoretical and Energy Biomass Potential of Heat and Electricity Production in Kosovo
Authors: Sertolli Ardit , Bai Attila , Gabnai Zoltán , Mizik Tamás , Pestisha Albiona
Published: October 2023

The energy use of residues from agriculture, forestry, and solid waste can foster the transition towards a more renewable energy supply. This paper analyzes the energy potential of the above-mentioned sources for energy applications in Kosovo. The analysis is based on statistical data from different studies and reports, analyzing and calculating them to determine the theoretical and energy biomass potential. Kosovo can increase its self-sufficiency by taking advantage of its rich but under-utilized potential of biomass energy sources. This is a novelty study in this area, considering Kosovo lignite-dominated heat energy and electricity consumption and the available special literature. According to our estimates, the theoretical potential is 6.13 million tons/year, while the biomass energy potential should be around 4.57 million tons/year, including approximately 74.6% of biomass, which can be used for energy needs (heating and electricity). Based on the data and calculations, the available and usable potential shows biomass as an energy source with high potential in Kosovo; its share is very low, but it is reasonable to grow for both environmental and economic reasons.
Electric Vehicle Fleet Management for a Prosumer Building with Renewable Generation
Authors: Fresia Matteo , Bracco Stefano
Published: October 2023

The integration of renewable energy systems in buildings leads to a reduction in energy bills for end users and a reduction in the carbon footprint of such buildings, usually referred to as prosumers. In addition, the installation of charging points for the electric vehicles of people working or living in these buildings can further improve the energy efficiency of the whole system if innovative technologies, such as vehicle-to-building (V2B) technologies, are implemented. The aim of this paper is to present an Energy Management System (EMS) based on mathematical programming that has been developed to optimally manage a prosumer building equipped with photovoltaics, a micro wind turbine and several charging points for electric vehicles. Capabilities curves of renewable power plant inverters are modelled within the EMS, as well as the possibility to apply power curtailment and V2B. The use of V2B technology reduces the amount of electricity purchased from the public grid, while the use of smart inverters for the power plants allows zero reactive power to be drawn from the grid. Levelized cost of electricity (LCOE) is used to quantify curtailment costs, while penalties on reactive power absorption from the distribution network are evaluated in accordance with the current regulatory framework. Specifically, the model is applied to a prosumer building owned by the postal service in a large city in Italy. The paper reports the main results of the study and proposes a sensitivity analysis on the number of charging stations and vehicles, as well as on the consideration of different typical days characterized by different load and generation profiles. This paper also investigates how errors in forecasting energy production from renewable sources impact the optimal operation of the whole system.
Assessment of a Francis Micro Hydro Turbine Performance Installed in a Wastewater Treatment Plant
Authors: Tomczyk Paweł , Mastalerek Krzysztof , Wiatkowski Mirosław , Kuriqi Alban , Jurasz Jakub
Published: October 2023

The purpose of this research work was to examine the hydroelectric potential of wastewater treatment plants by harnessing the kinetic and/or potential energy of treated wastewater for electricity generation. Such a concept encapsulates the essence of renewable energy and resonates with international sustainable development mandates and climate change adaptation strategies. The primary objective was to analyze the performance parameters of the Francis turbine, a key component of this energy generation system. An experimental analysis encompassed model tests on the Francis turbine, simulating varied flow conditions using the GUNT turbine. Additionally, historical data from the Toruń Wastewater Treatment Plant (WWTP) 2018 annual wastewater discharge were employed to validate the findings and shed light on real-world applications. The tested efficiency of the Francis turbine peaked at 64.76%, notably below the literature-reported 80%. The turbine system’s overall efficiency was approximately 53%, juxtaposed against the theoretical value of 66.35%. With respect to the Toruń WWTP data, the turbine’s power output was highest at 24.82 kW during maximum wastewater flow, resulting in a power production of 150.29 MWh per year. The observed turbine efficiencies were consistent with the previously documented range of 30% to 96%. The turbine displayed optimal outputs during heightened flow rates and maximized production at more frequent, lower flow rates throughout the year. Implementing such turbines in wastewater treatment plants not only aligns with global renewable energy goals but also boasts lower construction costs and environmental impacts, primarily due to the utilization of existing infrastructure. Furthermore, wastewater flow consistency counters the seasonal variability seen in conventional water treatment plants. These findings pave the way for more energy-efficient design recommendations for turbines within wastewater treatment and hydropower plants.
Settling Flow Details in the Flash Smelting Furnace—A CFD-DEM Simulation Study
Authors: Jylhä Jani-Petteri , Jokilaakso Ari
Published: October 2023

The flash smelting furnace has previously been simulated using computational fluid dynamics (CFD). A new approach is to combine CFD and the discrete element method (DEM) for more detailed simulations of the different phenomena that occur as copper matte droplets settle through a slag layer. One of the most important phenomena found is the formation of a channeling flow which carries matte droplets faster through the slag. However, such phenomena cannot be directly observed in the flash smelting furnace settler due to the extreme temperatures of the opaque molten slag inside the furnace, therefore alternative methods are required for validating the phenomenon. In this work, the simulated channeling flow is validated with a sphere–oil model. The phenomenon was similar in all of the studied cases, although in the experimental setup the spheres settled faster in the oil model than in the simulations. The differences were most likely caused by the cohesion of the spheres and slight differences in the properties provided by the manufacturer and real properties for the oil and the spheres, and by the fact that simulation ignores surface tension and changing air–oil and water–oil interfaces.
Dynamics of Growth in Purebred Pacu (Piaractus mesopotamicus) and Tambaqui (Colossoma macropomum), and Their Reciprocal Hybrids, under Varied Feeding Programs: Insights from Nonlinear Models
Authors: Gervaz Woshinghton Rocha , Leonardo Antônio Fernando , Hashimoto Diogo Teruo , Allaman Ivan Bezerra , Lattanzi Gabriel Rinaldi , Reis Neto Rafael Vilhena
Published: October 2023

We evaluated the growth performance of pacu and tambaqui and their reciprocal hybrids (tambacu and paqui) under different feeding programs. We raised 30 individuals from each species and their respective crosses, distributing them into three replicate cages with 10 individuals each. Throughout the 5-month experimental period, the fish were weighed six times while exposed to diverse feeding regimens involving three commercial diets with varying combinations of crude protein (CP) levels: 24%, 28%, and 32%. Growth curves were adjusted using nonlinear models. The evaluation period was insufficient for adjusting the logistic model for the tambaqui and paqui treatments with the highest initial protein intake. Pure pacu had a higher (p < 0.05) growth rate (k = 0.0185) than in the tambacu hybrid (k = 0.0134) and proved to have an early performance since animals from this group reached their weight at inflection faster. Despite growing more slowly, tambacu reached a higher (p < 0.05) final weight (A = 1137.12) than in the pacu (A = 889.12). Among the feeding programs, animals that received less CP showed greater growth potential; however, longer evaluation is important to verify if the initial CP intake has no significant positive effect on fish growth.
Characteristics of the Accessible Chromatin Landscape and Transcriptome under Different Temperature Stresses in Bemisia tabaci
Authors: Shen Xiaona , Wang Xiaodi , Yang Nianwan , Wan Fanghao , Lü Zhichuang , Guo Jianying , Liu Wanxue
Published: October 2023

Bemisia tabaci is an important invasive pest with worldwide distribution and strong temperature tolerance. Previous studies have shown that temperature tolerance varies significantly between the different invasive populations. Several key factors involved in epigenetic regulation have been identified and verified in B. tabaci; therefore, epigenetic adaptation mechanisms may also exist. This study aimed to detect changes in the chromatin accessibility landscape and genome-wide transcriptome under different temperature stresses in B. tabaci. Assay for transposase-accessible chromatin with high-throughput sequencing and RNA-seq analyses indicated that transcriptional activity of the genes strongly correlates with chromatin accessibility. Chromatin transcription-activated gene expression regulation is dominant during high-temperature stress in B. tabaci, mainly through the transcriptional repression of genes related to low-temperature stress resistance. Furthermore, B. tabaci resists low-temperature stress by regulating enzyme activities and withstands high-temperature stress by regulating metabolism and synthesis of organic substances, both achieved by altering chromatin accessibility. In summary, this study provides a theoretical basis for exploring changes in gene expression and chromatin accessibility under different temperature stresses, offering a new approach to unravelling regulatory mechanisms underlying the onset of molecular regulation in response to various temperature stress conditions.
Predicting Falls in Musculoskeletal Rehabilitation: A Retrospective Multisite Study
Authors: Scarabel Luca , Scarpina Federica , Ruggieri Graziano , Schiavone Nicola , Limoni Costanzo
Published: October 2023

Background. Fall represents one of the highest concerns in the healthcare system, especially in medical rehabilitation settings. However, there is a lack of instruments for the assessment of risk falls in the context of musculoskeletal rehabilitation. Methods. This retrospective multisite study aimed to assess the sensitivity and specificity of four fall risk assessment tools (the Functional Independence Measure, the Fall Risk Assessment, the Schmid Fall Risk Assessment Tool, and the ePA-AC) in predicting falls in patients admitted to musculoskeletal rehabilitation in Swiss inpatient facilities. Results. The data relative to 6970 individuals (61.5% females) were analyzed and 685 (9.83% of patients) fall events were registered. The area under the curve (AUC) relative to the Functional Independence Measure was 0.689, 0.66 for the Fall Risk Assessment, 0.641 for the Schmid Fall Risk Assessment Tool, and 0.675 for the ePA-AC. Among the four tools, the Functional Independence Measure had an acceptable discriminatory power in distinguishing between significant events (i.e., patients’ falls) and non-events (no falls). Conclusion. None of the assessed tools showed highly satisfying levels of statistical sensitivity or sensibility. However, the Functional Independence Measure could be used to assess the fall risk assessment in musculoskeletal rehabilitation settings, although with some caution, since this questionnaire was not designed for this diagnostic purpose. We strongly suggest urgently designing a tool for risk assessment that is specific to this population and the rehabilitative setting.
Integrated Transcriptomic and Metabolomic Analysis Reveal the Underlying Mechanism of Anthocyanin Biosynthesis in Toona sinensis Leaves
Authors: Xu Jing , Fan Yanru , Han Xiaojiao , Pan Huanhuan , Dai Jianhua , Wei Yi , Zhuo Renying , Liu Jun
Published: October 2023

Toona sinensis, commonly known as Chinese Toon, is a plant species that possesses noteworthy value as a tree and vegetable. Its tender young buds exhibit a diverse range of colors, primarily determined by the presence and composition of anthocyanins and flavonoids. However, the underlying mechanisms of anthocyanin biosynthesis in Toona sinensis have been rarely reported. To explore the related genes and metabolites associated with composition of leaf color, we conducted an analysis of the transcriptome and metabolome of five distinct Toona clones. The results showed that differentially expressed genes and metabolites involved in anthocyanin biosynthesis pathway were mainly enriched. A conjoint analysis of transcripts and metabolites was carried out in JFC (red) and LFC (green), resulting in the identification of 510 genes and 23 anthocyanin-related metabolites with a positive correlation coefficient greater than 0.8. Among these genes and metabolites, 23 transcription factors and phytohormone-related genes showed strong coefficients with 13 anthocyanin derivates, which mainly belonged to the stable types of delphinidin, cyanidin, peonidin. The core derivative was found to be Cyanidin-3-O-arabinoside, which was present in JFC at 520.93 times the abundance compared to LFC. Additionally, the regulatory network and relative expression levels of genes revealed that the structural genes DFR, ANS, and UFGT1 might be directly or indirectly regulated by the transcription factors SOC1 (MADS-box), CPC (MYB), and bHLH162 (bHLH) to control the accumulation of anthocyanin. The expression of these genes was significantly higher in red clones compared to green clones. Furthermore, RNA-seq results accurately reflected the true expression levels of genes. Overall, this study provides a foundation for future research aimed at manipulating anthocyanin biosynthesis to improve plant coloration or to derive human health benefits.
Short-Term Inhibition of Translation by Cycloheximide Concurrently Affects Mitochondrial Function and Insulin Secretion in Islets from Female Mice
Authors: Alshafei Mohammed , Schulze Torben , Morsi Mai , Panten Uwe , Rustenbeck Ingo
Published: October 2023

Since glucose stimulates protein biosynthesis in beta cells concomitantly with the stimulation of insulin release, the possible interaction of both processes was explored. The protein biosynthesis was inhibited by 10 μM cycloheximide (CHX) 60 min prior to the stimulation of perifused, freshly isolated or 22 h-cultured NMRI mouse islets. CHX reduced the insulinotropic effect of 25 mM glucose or 500 μM tolbutamide in fresh but not in cultured islets. In cultured islets the second phase of glucose stimulation was even enhanced. In fresh and in cultured islets CHX strongly reduced the content of proinsulin, but not of insulin, and moderately diminished the [Ca2+]i increase during stimulation. The oxygen consumption rate (OCR) of fresh islets was about 50% higher than that of cultured islets at basal glucose and was significantly increased by glucose but not tolbutamide. In fresh, but not in cultured, islets CHX diminished the glucose-induced OCR increase and changes in the NAD(P)H- and FAD-autofluorescence. It is concluded that short-term CHX exposure interferes with the signal function of the mitochondria, which have different working conditions in fresh and in cultured islets. The interference may not be an off-target effect but may result from the inhibited cytosolic synthesis of mitochondrial proteins.
Histopathological Gap in Aortic Diseases: A Prospective Analysis
Authors: Banceu Cosmin Marian , Gurzu Simona , Satala Catalin-Bogdan , Ghiga Dana , Neamtu Mihai Halic , Voth Vladimir , Liebrich Markus , Suciu Horatiu
Published: October 2023

Aortic dissection (AD) is a critical cardiovascular condition with the potential for devastating consequences. This study evaluated the histological changes in the aorta wall in patients with AD and aortic aneurysm (AA) who received surgical aortic replacement. Histopathological data showed that modifications of the media layer (p = 0.0197), myxomatous aspect (p = 0.0001), and subendothelial layer degeneration (p = 0.0107) were more frequently seen in AA versus AD samples. Patients with AA were approximately twice as likely to develop histological changes than those with AD (p = 0.0037). Patients with moderate or severe medial degeneration had a higher chance of developing AD (p = 0.0001). Because the histopathological score proved to be a predictor of both in-hospital and overall mortality, its evaluation should become the standard of care in any patients who undergo aortic replacement. Individualized postoperative management might be influenced by the histopathological aspect of the aortic layer.
The Integration of Genome-Wide DNA Methylation and Transcriptomics Identifies the Potential Genes That Regulate the Development of Skeletal Muscles in Ducks
Authors: Lu Yinglin , Zhou Jing , Li Fan , Cao Heng , Zhang Xingyu , Yu Debing , He Zongliang , Ji Hongjie , Lv Kunpeng , Wu Guansuo , Yu Minli
Published: October 2023

DNA methylation is a pivotal epigenetic regulatory mechanism in the development of skeletal muscles. Nonetheless, the regulators responsible for DNA methylation in the development of embryonic duck skeletal muscles remain unknown. In the present study, whole genome bisulfite sequencing (WGBS) and transcriptome sequencing were conducted on the skeletal muscles of embryonic day 21 (E21) and day 28 (E28) ducks. The DNA methylation pattern was found to fall mainly within the cytosine-guanine (CG) context, with high methylation levels in the intron, exon, and promoter regions. Overall, 7902 differentially methylated regions (DMRs) were identified, which corresponded to 3174 differentially methylated genes (DMGs). By using integrative analysis of both WGBS with transcriptomics, we identified 1072 genes that are DMGs that are negatively associated with differentially expressed genes (DEGs). The gene ontology (GO) analysis revealed significant enrichment in phosphorylation, kinase activity, phosphotransferase activity, alcohol-based receptors, and binding to cytoskeletal proteins. The Kyoto Encyclopedia of Genes and Genomes (KEGGs) analysis showed significant enrichment in MAPK signaling, Wnt signaling, apelin signaling, insulin signaling, and FoxO signaling. The screening of enriched genes showed that hyper-methylation inhibited the expression of Idh3a, Got1, Bcl2, Mylk2, Klf2, Erbin, and Klhl38, and hypo-methylation stimulated the expression of Col22a1, Dnmt3b, Fn1, E2f1, Rprm, and Wfikkn1. Further predictions showed that the CpG islands in the promoters of Klhl38, Klf2, Erbin, Mylk2, and Got1 may play a crucial role in regulating the development of skeletal muscles. This study provides new insights into the epigenetic regulation of the development of duck skeletal muscles.
Flavonoid-Rich Sambucus nigra Berry Extract Enhances Nrf2/HO-1 Signaling Pathway Activation and Exerts Antiulcerative Effects In Vivo
Authors: Cicek Betul , Danısman Betul , Yildirim Serkan , Yuce Neslihan , Nikitovic Dragana , Bolat Ismail , Kuzucu Mehmet , Ceyran Ertuğrul , Bardas Ebru , Golokhvast Kirill S. , Tsatsakis Aristidis , Taghizadehghalehjoughi Ali
Published: October 2023

Sambucus nigra (SN) berry extract is characterized by high antioxidant and anti-inflammatory activity. The current study aimed to investigate the effect of SN berry extract against indomethacin (IND)-induced gastric ulcer in rats and the mechanism involved. SN berry extract alleviated IND-induced gastric ulcers, as shown by assessing pathological manifestations in the gastric mucosa. These protective effects are attributed to attenuated oxidative damage to the gastric mucosa, correlated to increased activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), enhanced glutathione (GSH) levels, total antioxidant capacity (TAC), and upregulation of the Nrf2/HO-1 cascade. Moreover, oxidative stress markers, including malondialdehyde (MDA) and total oxidant status (TOS), were downregulated in SN-extract-treated animals. Furthermore, SN berry extract suppressed gastric mucosal inflammation by downregulating interleukin (IL)-33, IL-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) levels, and attenuating myeloperoxidase (MPO) activity. The protective effects of SN berry extract were similar to those exerted by esomeprazole (ESO), an acid-secretion-suppressive drug. In conclusion, SN berry extract has antiulcerative effects, alleviating oxidative stress and inflammation.
Evaluation of the Clinical Variables Affecting Attachment Reproduction Accuracy during Clear Aligner Therapy
Authors: Bellocchio Angela Mirea , Portelli Marco , Ciraolo Ludovica , Ciancio Elia , Militi Angela , Peditto Matteo , Barbera Serena , Nucera Riccardo
Published: October 2023

Background: The purpose of this study was to evaluate some of the clinical variables that influence the accuracy of reproducing the planned attachment shape. The following clinical variables were considered: the template material, type of composite, and pressure application on the template during attachment curing. Methods: In this study, the evaluated materials for the thermoplastic transfer template construction are Erkolen 0.8 (polyethylene: PE) and Erkodur 0.8 (polyethylene terephthalate glycol—PET-G), and two types of composite resins: Enaflow (light-curing low-viscosity composite resin) and Enamel plus dentina HRI (light-curing high-viscosity composite resin). Two different light-curing lamps were used: Valo cordless color with no pressure and push light pressure (SCS). The 26 models included in the study were imported into the 3 Shape Ortho System 2022 (ver. 85.0.20 3 Shape, Denmark), and attachments were virtually placed on the dental elements of the first premolar and on both sides of the first upper molars. The accuracy of the attachment reproduction was evaluated through linear and angular evaluations against the reference model (MCAD). Three physical models were obtained: model A (MA), which was printed with attachments; model B (MB) with attachments made with a PE template; and model C (MC) with attachments made with a PET-G template. Results: The results showed statistically significant differences (p < 0.05) between the PE and PET-G templates with greater precision using the PET-G template. Statistically significant differences (p < 0.05) were found among the high-viscosity composite and low-viscosity composite with pressure curing. Conclusions: In light of the obtained data, using a PET-G template is recommended. The pressure application during composite curing reduces the reproduction accuracy with a low-viscosity composite.
Process Optimization of Polishing Titanium Alloy Material with a Pulsating Air Jet
Authors: Zhang Lei , Ding Chen , Bu Jianfa , Zhang Zhirui , Wang Yongguang , Fan Cheng
Published: October 2023

Titanium alloy is a widely used metal material, which can be applied in fields such as healthcare, petroleum exploration, aerospace, etc. In this paper, a new method for polishing the titanium alloy by a pulsating air jet is proposed. Compared with traditional abrasive jet polishing, this method has the advantages of simple structure, low nozzle wear, and high polishing flexibility. The working principle and material removal mechanism of the pulsating air jet polishing (PAJP) are introduced. Combined with orthogonal experiments, range analysis and variance analysis were used to find out the influence degree of each process parameter on the PAJP of titanium alloy, and the optimal level of each parameter was found. Through the experiments, a prediction model of surface roughness was established by regression analysis, and the predicted value was compared with the measured value. The maximum relative error of the prediction model was 10.3%, and the minimum relative error was 1.1%. The average relative error was 6.2%.
Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in Litopenaeus vannamei by Sulforaphane
Authors: Luo Junliang , Huang Yongxiong , Chen Yanghui , Yuan Yunhao , Li Guojian , Cai Shuanghu , Jian Jichang , Yang Shiping
Published: October 2023

Heme oxygenase-1 (HO-1), which could be highly induced under the stimulation of oxidative stress, functions in reducing the damage caused by oxidative stress, and sulforaphane (SFN) is an antioxidant. This study aims to investigate whether HO-1 is involved in the repair of oxidative damage induced by oxidized fish oil (OFO) in Litopenaeus vannamei by sulforaphane (SFN). The oxidative stress model of L. vannamei was established by feeding OFO feed (OFO accounts for 6%), and they were divided into the following four groups: control group (injected with dsRNA-EGFP and fed with common feed), dsRNA-HO-1 group (dsRNA-HO-1, common feed), dsRNA-HO-1 + SFN group (dsRNA-HO-1, supplement 50 mg kg−1 SFN feed), and SFN group (dsRNA-EGFP, supplement 50 mg kg−1 SFN feed). The results showed that the expression level of HO-1 in the dsRNA-HO-1 + SFN group was significantly increased compared with the dsRNA-HO-1 group (p < 0.05). The activities of SOD in muscle and GPX in hepatopancreas and serum of the dsRNA-HO-1 group were significantly lower than those of the control group, and MDA content in the dsRNA-HO-1 group was the highest among the four groups. However, SFN treatment increased the activities of GPX and SOD in hepatopancreas, muscle, and serum and significantly reduced the content of MDA (p < 0.05). SFN activated HO-1, upregulated the expression of antioxidant-related genes (CAT, SOD, GST, GPX, Trx, HIF-1α, Nrf2, prx 2, Hsp 70), and autophagy genes (ATG 3, ATG 5), and stabilized the expression of apoptosis genes (caspase 2, caspase 3) in the hepatopancreas (p < 0.05). In addition, knocking down HO-1 aggravated the vacuolation of hepatopancreas and increased the apoptosis of hepatopancreas, while the supplement of SFN could repair the vacuolation of hepatopancreas and reduce the apoptosis signal. In summary, HO-1 is involved in the repair of the oxidative damage induced by OFO in L. vannamei by SFN.
Elderly-Onset Rheumatoid Arthritis: Characteristics and Treatment Options
Authors: Pavlov-Dolijanovic Slavica , Bogojevic Milan , Nozica-Radulovic Tatjana , Radunovic Goran , Mujovic Natasa
Published: October 2023

Elderly-onset rheumatoid arthritis (EORA) is a distinct clinical entity defined as the onset of rheumatoid arthritis (RA) in individuals aged over 60 years. EORA presents unique clinical features, including a more equitable distribution of sexes, a potential predilection for male involvement, a higher incidence of acute onset characterized by constitutional symptoms, a propensity for systemic manifestations, elevated sedimentation rates at disease onset, a reduced occurrence of rheumatoid factor positivity, increased titers of anti-citrullinated protein antibodies, a preference for involvement of large joints, elevated disease activity, the presence of bone erosions, and heightened patient disability. RA is recognized to consist of three partially overlapping subsets. One subset mirrors the classical RA clinical presentation, while the remaining subsets exhibit either a polymyalgia rheumatica-like phenotype or present with remitting seronegative symmetrical synovitis accompanied by pitting edema syndrome. In the initial stages of EORA management, non-steroidal anti-inflammatory drugs (NSAIDs) are not typically the first-line treatment choice, because seniors are much more prone to develop side effects due to NSAIDs, and the use of NSAIDs is in reality contraindicated to the majority of seniors due to comorbidities. Disease-modifying antirheumatic drugs (DMARDs), frequently methotrexate, are introduced immediately after the diagnosis is made. In cases where elderly patients demonstrate resistance to conventional DMARD therapy, the introduction of biological or targeted synthetic DMARDs becomes a viable treatment option. EORA presents a unique clinical profile, necessitating tailored treatment strategies. Our study emphasizes the challenges of NSAID use in seniors, highlighting the imperative shift toward DMARDs such as methotrexate. Future research should explore personalized DMARD approaches based on disease activity, comorbidities, and safety considerations, aiming to optimize treatment outcomes and minimize glucocorticoid reliance, thereby enhancing the quality of care for EORA patients.
Anisakiasis in the Upper Esophagus: A Case Report
Authors: Kamba Eiji , Murakami Takashi , Ueyama Hiroya , Shibuya Tomoyoshi , Hojo Mariko , Yamaji Ken , Nagahara Akihito
Published: October 2023

Anisakiasis is caused by consuming raw fish contaminated with Anisakis sp. larvae and is extremely rare, especially when originating in the esophagus. We present a case of esophageal anisakiasis in a 61-year-old male who experienced severe precordial pain and radiating discomfort to the neck after consuming raw fish sashimi. Upper gastrointestinal endoscopy revealed the presence of a larva in the upper esophagus. On the basis of anatomo-morphological features, the worm was provisionally identified as Anisakis sp. and was easily extracted with forceps, which led to a prompt improvement in the patient’s symptoms. This case highlights the importance of considering anisakiasis as a differential diagnosis in patients with gastrointestinal symptoms and a history of consuming raw fish.
A Comprehensive Bibliographic Review Concerning the Efficacy of Organic Acids for Chemical Peels Treating Acne Vulgaris
Authors: Măgerușan Șoimița Emiliana , Hancu Gabriel , Rusu Aura
Published: October 2023

Acne vulgaris stands out as the most prevalent skin disorder among teenagers and young adults, causing physical discomfort and considerable economic and psychological burdens on individuals and society. A wide range of topical and systemic therapies are available in acne treatment. Chemical peeling is a skin resurfacing technique designed to rebuild healthy skin using exfoliating substances, a simple and affordable process with various dermatological uses. Chemical peels, classified as superficial, medium, and deep, have been utilized for acne vulgaris and multiple other skin issues. In these chemical peels, a diverse range of chemical substances is employed, each with its unique mode of action. Among these, α-hydroxy and β-hydroxy acids have gathered attention for their efficacy in reducing acne lesions and enhancing overall skin appearance. Acids, such as salicylic acid, glycolic acid, or lactic acid, are commonly used in chemical peels due to their exfoliating and sebum-regulating properties. Despite the widespread use of these acids, there exists a lack of consensus regarding the most effective acid type and concentration for treating acne-prone skin. This review aims to bridge this knowledge gap by evaluating the effectiveness and safety of various organic acids used in chemical peels specifically for acne-prone skin. The findings of this comprehensive bibliographic review indicate that organic acid-based chemical peels represent effective and safe treatment options for individuals with acne-prone skin. Their adaptability sets these treatments apart; the choice of organic acid can be tailored to meet individual patient needs and tolerability levels. This personalized approach ensures that patients receive optimal care while minimizing the risks associated with the treatment. As research in this field progresses, it is anticipated that a more nuanced understanding of the ideal acid type and concentration will emerge, further enhancing the efficacy and safety of chemical peels for acne-prone skin.
Nanoscale Dispersion of Carbon Nanotubes in a Metal Matrix to Boost Thermal and Electrical Conductivity via Facile Ball Milling Techniques
Authors: Li Bin , Zhou Lihua , Wang Bo , Yin Maoshu , Qian Yong , Shi Xianglei , Guo Zhejun , Han Zhao , Hu Nantao , Sun Lijie
Published: October 2023

Carbon nanotube (CNT)/metal composites have attracted much attention due to their enhanced electrical and thermal performance. How to achieve the scalable fabrication of composites with efficient dispersion of CNTs to boost their performance remains a challenge for their wide realistic applications. Herein, the nanoscale dispersion of CNTs in the Stannum (Sn) matrix to boost thermal and electrical conductivity via facile ball milling techniques was demonstrated. The results revealed that CNTs were tightly attached to metal Sn, resulting in a much lower resistivity than that of bare Sn. The resistivity of Sn with 1 wt.% and 2 wt.% CNTs was 0.087 mΩ·cm and 0.056 mΩ·cm, respectively. The theoretical calculation showed that there was an electronic state near the Fermi level, suggesting its electrical conductivity had been improved to a certain extent. In addition, the thermal conductivity of Sn with 2 wt.% CNTs was 1.255 W·m−1·K−1. Moreover, Young’s modulus of the composites with CNTs mass fraction of 10 wt.% had low values (0.933 MPa) under low strain conditions, indicating the composite shows good potential for various applications with different flexible requirements. The good electrical and thermal conductive CNT networks were formed in the metal matrix via facile ball milling techniques. This strategy can provide guidance for designing high-performance metal samples and holds a broad application potential in electronic packaging and other fields.