bluuebunny/crossref_metadata_2025 huggingface dataset:
Promoting Safety Climate Training for Migrant Workers through Non-Technical Skills: A Step Forward to Inclusion
Authors: Morando Martina , Brullo Leonardo
Published: February 2022

Currently, the number of migrant workers is significantly high and represents a global issue. The impact of the negative aspects of employment and psychosocial risk factors in the workplace on migrants’ health and well-being has been underestimated. This study focused first on a perceptions analysis of the diversity climate, safety climate and experiences of discrimination of migrant workers, and then on testing whether and how the contents and meanings of ad hoc training were transferable and transferred to their workplace. Through a program of 4 phases (pre-screening, intervention, assessment and follow-up) and a series of interviews and focus groups, 24 participants were recruited for the study. Consistent with literature, the findings confirmed a poor perception of diversity and safety climate, and several discrimination experiences. The training outcomes showed an improvement of the individual perception of safety, motivation and self-efficacy. In contrast, the work context is still perceived as closed and constraining, which makes complete transferability hard to achieve. Results are quite encouraging and bear out the need for training and the first positive effects on working conditions and quality of life. This study is one of the first attempts in Italy to integrate the analysis of migrants’ labor situation, training and its evaluation. Future studies are required.
Study of the Fracture Behavior of TiN and TiC Inclusions in NM550 Wear-Resistant Steel during the Tensile Process
Authors: Liu Denghui , Wang Zhongyang , Liu Jingjing , Wang Zhenlong , Zuo Xiurong
Published: February 2022

NM550 wear-resistant steel is widely used in large-scale engineering and mining machinery under extremely harsh working conditions. In NM550 steel, the addition of Ti can cause the formation of micron-scale TiN and TiC inclusions, easily triggering cleavage fractures. The fracture behavior and precipitation rule of micron-scale TiN and TiC inclusions on the tensile process in NM550 steel was investigated by scanning electron microscopy, transmission electron microscopy, and energy spectrum analysis combined with thermodynamic theory. The TiN precipitated in the solid–liquid two-phase region at a precipitation temperature of 1710 K, whereas that of TiC was 1158 K along the austenite grain boundary. The sizes of the TiN precipitated in the liquid phase and the TiC precipitated in austenite were both at the micron scale, which is prone to cleavage fracture during the stretching process. Under tensile stress, microcracks were first initiated at the TiN inclusion, which were further separated forming a hole, whereas the TiC inclusion was divided into two sections with a long and narrow gap formed between the substrates. The sizes of the TiN and TiC were related to the cooling rate, Ti, and N contents. The larger the cooling rate and the lesser the Ti and N content, the smaller the TiN and TiC sizes.
Microstructure Characteristics and Corrosion Behaviors of Mg-2Zn-2Er-0.3Zr-0.3Mn Alloy under Various Rolling Reductions
Authors: Zheng Yaqi , Zhang Yuan , Liu Yun , Tian Yaqiang , Zheng Xiaoping , Chen Liansheng
Published: February 2022

The poor corrosion resistance of magnesium alloys is one of the major obstacles to their widespread applications in the engineering field and the medical field. A hot deformation process is the main way to improve the corrosion resistance of magnesium alloys. In the present study, X-ray photoelectron spectroscopy (XPS), a scanning electron microscope (SEM), an electron probe micro-analyzer (EPMA) and X-ray diffraction (XRD) were used to investigate the micro-galvanic corrosion behavior and film protection mechanism of hot-rolled Mg-2Zn-2Er-0.3Zr-0.3Mn under 25%, 50% and 75% thickness reductions in Hank’s solution. The results revealed that the best corrosion resistance was obtained in the alloy under a 75% thickness reduction, with a corrosion rate of 0.85 mm/y. The improvement in anti-corrosion was due to the coupling effect of a refined microstructure and dense degradation film on the large deformation hot-rolled alloy. Furthermore, the elements Mn and Er participated in the film formation and stabilized the film structure.
Evolution of the Structural State and Properties of 32CrB4 Steel during the Production of High-Strength Fasteners
Authors: Stepanov Alexey , Koldaev Anton , Arutyunyan Nataliya , Zaitsev Alexander
Published: February 2022

For the production of high-strength fasteners, bar sections are used, which must withstand significant cold plastic deformation and, therefore, are subjected to a special heat treatment. In the present work, the dependences of the microstructure and mechanical properties of round bars with diameters of 17 mm and 21 mm made of 32CrB4 steel on the heat treatment modes are established. During the study, the methods of optical and scanning electron microscopy, mechanical tests, and hardness measurements were used. Dependences of the degree of spheroidization of the ferrite–pearlite structure and mechanical properties on the spheroidizing annealing mode have been established. It is shown that preliminary obtainment of a bainitic or martensitic structure of rolled products after hot-rolling of steel accelerates the process of pearlite spheroidization. Additional heat treatment, including austenitizing, quenching, and tempering, allows obtaining fasteners of various strength classes: 8.8, 9.8, and 10.9. However, with a guaranteed receipt of strength characteristics for all strength classes, including 12.9, the problem arises of achieving the required values of the relative elongation of steel.
Joining of Ti6Al4V to Al2O3 Using Nanomultilayers
Authors: Silva Marcionilo , Ramos Ana Sofia , Vieira Maria Teresa , Simões Sónia
Published: February 2022

Diffusion bonding of Ti6Al4V to Al2O3 using Ni/Ti reactive nanomultilayers as interlayer material was investigated. For this purpose, Ni/Ti multilayer thin films with 12, 25, and 60 nm modulation periods (bilayer thickness) were deposited by d.c. magnetron sputtering onto the base materials’ surface. The joints were processed at 750 and 800 °C with a dwell time of 60 min and under a pressure of 5 MPa. Microstructural characterization of the interfaces was conducted by scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDS), and electron backscatter diffraction (EBSD). The mechanical characterization of the joints was performed by nanoindentation, and hardness and reduced Young’s modulus distribution maps were obtained across the interfaces. The joints processed at 800 °C using the three modulation periods were successful, showing the feasibility of using these nanolayered films to improve the diffusion bonding of dissimilar materials. Using modulation periods of 25 and 60 nm, it was also possible to reduce the bonding temperature to 750 °C and obtain a sound interface. The interfaces are mainly composed of NiTi and NiTi2 phases. The nanoindentation experiments revealed that the hardness and reduced Young’s modulus at the interfaces reflect the observed microstructure.
An Ionically Conductive, Self-Powered and Stable Organogel for Pressure Sensing
Authors: Wang Li , Wang Zhengduo , Li Yingtao , Luo Yu , Lu Bingheng , Gao Yiyang , Yu Wei , Gao Guoxin , Ding Shujiang
Published: February 2022

Gel-based ionic conductors are promising candidates for flexible electronics, serving as stretchable sensors or electrodes. However, most of them suffer from a short operating life, low conductivity and rely on an external power supply, limiting their practical application. Herein, we report a stable organogel ionic conductor with high conductivity and self-powering ability. Briefly, lithium trifluoromethanesulfonate, as a conductive salt, provides high conductivity and the poly(1,1-difluoroethylene) layers, as a self-powering system, supply stable energy output under the influence of pressure. Moreover, the proposed conductors withstand long-term and multi-cycle durability tests. The prepared auxiliary training device can withstand the impact of a basketball and detect the impact force, showing potential in passive sensing during practical applications.
Compact SnO2/Mesoporous TiO2 Bilayer Electron Transport Layer for Perovskite Solar Cells Fabricated at Low Process Temperature
Authors: Lee Junyeong , Kim Jongbok , Kim Chang-Su , Jo Sungjin
Published: February 2022

Charge transport layers have been found to be crucial for high-performance perovskite solar cells (PSCs). SnO2 has been extensively investigated as an alternative material for the traditional TiO2 electron transport layer (ETL). The challenges facing the successful application of SnO2 ETLs are degradation during the high-temperature process and voltage loss due to the lower conduction band. To achieve highly efficient PSCs using a SnO2 ETL, low-temperature-processed mesoporous TiO2 (LT m-TiO2) was combined with compact SnO2 to construct a bilayer ETL. The use of LT m-TiO2 can prevent the degradation of SnO2 as well as enlarge the interfacial contacts between the light-absorbing layer and the ETL. SnO2/TiO2 bilayer-based PSCs showed much higher power conversion efficiency than single SnO2 ETL-based PSCs.
ZnO Electrodeposition Model for Morphology Control
Authors: Orozco-Messana Javier , Camaratta Rubens
Published: February 2022

In this research, a model for electrodeposition of zinc oxide (ZnO) nanostructures over indium-doped tin-oxide (ITO) glass using pulsed current and zinc chloride as source of zinc was proposed. For the model, reactions kinetics rate constants were evaluated by obtaining the reaction product solid mass of the various species through time using an electrochemical quartz crystal microbalance (EQCM). To obtain a mathematical model of the electrodeposition using Ansys CFX 2D simulation software, the reaction kinetics rates were used to calculate mass transfer in the volume closest to the surface. The model was applied to the experimental electrodeposition conditions to validate its accuracy. Dense wurtzite nanostructures with controlled morphology were obtained on a indium-doped tin-oxide (ITO) glass. Sample characterization was performed using high-resolution field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) on focused ion beam milled (FIBed) sheets from wurtzite mono-crystals. Average crystallite size was evaluated by X-ray diffraction (XRD) using the Scherrer equation, and superficial areas were evaluated by Brunauer, Emmett, and Teller (BET) method. Through the experimental results, a chemical model was developed for the competing reactions based on the speciation of zinc considering pH evolution, and kinetic constants, on the oxygen rich aqueous environment. Owing to the model, an accurate prediction of thickness and type of electrodeposited layers, under given conditions, is achieved. This allows an excellent control of the optical properties of Wurtzite as a photon absorber, for an efficient separation of the electron-hole pair for conduction of the electric charges formed. The large surface area, and small wurtzite crystallites evenly distributed on the thin film electrodeposited over the ITO conductive layer are promising features for later dye-sensitized photovoltaic cell production.
Composite Nanoarchitectonics with CoS2 Nanoparticles Embedded in Graphene Sheets for an Anode for Lithium-Ion Batteries
Authors: Li Tongjun , Dong Hongyu , Shi Zhenpu , Yue Hongyun , Yin Yanhong , Li Xiangnan , Zhang Huishuang , Wu Xianli , Li Baojun , Yang Shuting
Published: February 2022

Cobalt sulfides are attractive as intriguing candidates for anodes in Lithium-ion batteries (LIBs) due to their unique chemical and physical properties. In this work, CoS2@rGO (CSG) was synthesized by a hydrothermal method. TEM showed that CoS2 nanoparticles have an average particle size of 40 nm and were uniformly embedded in the surface of rGO. The battery electrode was prepared with this nanocomposite material and the charge and discharge performance was tested. The specific capacity, rate, and cycle stability of the battery were systematically analyzed. In situ XRD was used to study the electrochemical transformation mechanism of the material. The test results shows that the first discharge specific capacity of this nanocomposite reaches 1176.1 mAhg−1, and the specific capacity retention rate is 61.5% after 100 cycles, which was 47.5% higher than that of the pure CoS2 nanomaterial. When the rate changes from 5.0 C to 0.2 C, the charge-discharge specific capacity of the nanocomposite material can almost be restored to the initial capacity. The above results show that the CSG nanocomposites as a lithium-ion battery anode electrode has a high reversible specific capacity, better rate performance, and excellent cycle performance.
Substantial Vitamin D Supplementation Is Required during the Prenatal Period to Improve Birth Outcomes
Authors: Hollis Bruce W. , Wagner Carol L.
Published: February 2022

Vitamin D supplementation during pregnancy has been studied since the early 1980′s and, while many clinical trials have been performed, we remain at a crossroads in our conclusions about vitamin D’s effects during pregnancy and the optimal dose and timing of supplementation [...]
A College Fast-Food Environment and Student Food and Beverage Choices: Developing an Integrated Database to Examine Food and Beverage Purchasing Choices among College Students
Authors: Racine Elizabeth F. , Schorno Rachel , Gholizadeh Shafie , Bably Morium Barakat , Hatami Faizeh , Stephens Casey , Zadrozny Wlodek , Schulkind Lisa , Paul Rajib
Published: February 2022

Universities typically offer residential students a variety of fast-food dining options as part of the student meal plan. When residential students make fast-food purchases on campus there is a digital record of the transaction which can be used to study food purchasing behavior. This study examines the association between student demographic, economic, and behavioral factors and the healthfulness of student fast-food purchases. The 3781 fast-food items sold at the University of North Carolina at Charlotte from fall 2016 to spring 2019 were given a Fast-Food Health Score. Each student participating in the university meal plan was given a Student Average Fast-Food Health Score; calculated by averaging the Fast-Food Health Scores associated with each food and beverage item the student purchased at a fast-food vendor, concession stand, or convenience store over a semester. This analysis included 14,367 students who generated 1,593,235 transactions valued at $10,757,110. Multivariate analyses were used to examine demographic, economic, and behavioral factors associated with Student Average Fast-Food Health Scores. Being of a low income, spending more money on fast-food items, and having a lower GPA were associated with lower Student Average Fast-Food Health Scores. Future research utilizing institutional food transaction data to study healthy food choices is warranted.
Natural Polysaccharide β-Glucan Protects against Doxorubicin-Induced Cardiotoxicity by Suppressing Oxidative Stress
Authors: Wang Xuan , Ji Yuting , Jin Dekui , Qi Jingyi , Hou Xuening , Zhao Wenting , Zhou Shuaishuai , Zhang Chengying , Luo Yongting , An Peng , Luo Junjie
Published: February 2022

Doxorubicin (DOXO) can be used to treat a variety of human tumors, but its clinical application is limited due to severe cardiotoxic side effect. Here, we explore the role of β-glucan in DOXO-induced cardiotoxicity in mice and study its underlying mechanism. When co-administered with DOXO, β-glucan was observed to prevent left ventricular dilation and fibrosis. In fact, DOXO reduces the activity of mitochondrial respiratory chain complex and enhances oxidative stress, which in turn impairs heart function. DOXO decreases the ATP production capacity of the heart and increases the ROS content, while β-glucan can restore the heart capacity and reduce oxidative stress. β-glucan also increases the activity of antioxidant enzymes GSH-PX and SOD, and reduces the level of MDA in the serum. In addition, the mRNAs of cardiac dysfunction marker genes ANP, BNP and Myh7 were significantly increased after DOXO induction, however, they did not increase when combined with β-glucan administration. In conclusion, our results indicate that β-glucan can improve the antioxidant capacity of the heart, thereby serving as a potential therapeutic strategy to prevent DOXO-induced cardiotoxicity.
Malnutrition in Hospitalized Old Patients: Screening and Diagnosis, Clinical Outcomes, and Management
Authors: Bellanti Francesco , lo Buglio Aurelio , Quiete Stefano , Vendemiale Gianluigi
Published: February 2022

Malnutrition in hospitalized patients heavily affects several clinical outcomes. The prevalence of malnutrition increases with age, comorbidities, and intensity of care in up to 90% of old populations. However, malnutrition frequently remains underdiagnosed and undertreated in the hospital. Thus, an accurate screening to identify patients at risk of malnutrition or malnourishment is determinant to elaborate a personal nutritional intervention. Several definitions of malnutrition were proposed in the last years, affecting the real frequency of nutritional disorders and the timing of intervention. Diagnosis of malnutrition needs a complete nutritional assessment, which is often challenging to perform during a hospital stay. For this purpose, various screening tools were proposed, allowing patients to be stratified according to the risk of malnutrition. The present review aims to summarize the actual evidence in terms of diagnosis, association with clinical outcomes, and management of malnutrition in a hospital setting.
Comparative Performance of Recombinant GRA6, GRA7, and GRA14 for the Serodetection of T. gondii Infection and Analysis of IgG Subclasses in Human Sera from the Philippines
Authors: Ybañez Rochelle Haidee , Nishikawa Yoshifumi
Published: February 2022

Highly specific and sensitive diagnostic methods are vital for the effective control and treatment of toxoplasmosis. Routine diagnosis is primarily serological because T. gondii infections stimulate persistently high IgG antibody responses. The sensitivity and specificity of methods are crucial factors for the proper diagnosis of toxoplasmosis, primarily dependent on the antigens used in different assays. In the present study, we compared the serodiagnostic performances of three recombinant dense granule antigens, namely, the GRA6, GRA7, and GRA14, to detect IgG antibodies against T. gondii in human sera from the Philippines. Moreover, we evaluated the IgG1, IgG2, IgG3, and IgG4 responses against the different recombinant antigens, which has not been performed previously. Our results revealed that the TgGRA7 has consistently displayed superior diagnostic capability, while TgGRA6 can be a satisfactory alternative antigen among the GRA proteins. Furthermore, IgG1 is the predominant subclass stimulated by the different recombinant antigens. This study’s results provide options to researchers and manufacturers to choose recombinant antigens suitable for their purpose.
The Potential of Nanomedicine to Unlock the Limitless Applications of mRNA
Authors: Taina-González Laura , de la Fuente María
Published: February 2022

The year 2020 was a turning point in the way society perceives science. Messenger RNA (mRNA) technology finally showed and shared its potential, starting a new era in medicine. However, there is no doubt that commercialization of these vaccines would not have been possible without nanotechnology, which has finally answered the long-term question of how to deliver mRNA in vivo. The aim of this review is to showcase the importance of this scientific milestone for the development of additional mRNA therapeutics. Firstly, we provide a full description of the marketed vaccine formulations and disclose LNPs’ pharmaceutical properties, including composition, structure, and manufacturing considerations Additionally, we review different types of lipid-based delivery technologies currently in preclinical and clinical development, namely lipoplexes and cationic nanoemulsions. Finally, we highlight the most promising clinical applications of mRNA in different fields such as vaccinology, immuno-oncology, gene therapy for rare genetic diseases and gene editing using CRISPR Cas9.
NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution
Authors: Kay Emma , Stulz Rouven , Becquart Cécile , Lovric Jelena , Tängemo Carolina , Thomen Aurélien , Baždarević Dženita , Najafinobar Neda , Dahlén Anders , Pielach Anna , Fernandez-Rodriguez Julia , Strömberg Roger , Ämmälä Carina , Andersson Shalini , Kurczy Michael
Published: February 2022

The delivery of antisense oligonucleotides (ASOs) to specific cell types via targeted endocytosis is challenging due to the low cell surface expression of target receptors and inefficient escape of ASOs from the endosomal pathway. Conjugating ASOs to glucagon-like peptide 1 (GLP1) leads to efficient target knockdown, specifically in pancreatic β-cells. It is presumed that ASOs dissociate from GLP1 intracellularly to enable an ASO interaction with its target RNA. It is unknown where or when this happens following GLP1-ASO binding to GLP1R and endocytosis. Here, we use correlative nanoscale secondary ion mass spectroscopy (NanoSIMS) and transmission electron microscopy to explore GLP1-ASO subcellular trafficking in GLP1R overexpressing HEK293 cells. We isotopically label both eGLP1 and ASO, which do not affect the eGLP1-ASO conjugate function. We found that the eGLP1 peptide and ASO are not detected at the same level in the same endosomes, within 30 min of GLP1R-HEK293 cell exposure to eGLP1-ASO. When we utilized different linker chemistry to stabilize the GLP1-ASO conjugate, we observed more ASO located with GLP1 compared to cell incubation with the less stable conjugate. Overall, our work suggests that the ASO separates from GLP1 relatively early in the endocytic pathway, and that linker chemistry might impact the GLP1-ASO function.
Aesthetics without Objects: Towards a Process-Oriented Aesthetic Perception
Authors: Perullo Nicola
Published: February 2022

In this paper, I suggest an aesthetic model that is consistent with anti-foundational scientific knowledge. How has an aesthetics without foundation to be configured? In contrast to the conventional subject/object model, with idealistic and subjective aesthetics, but also with object-oriented assumptions, I suggest that aesthetics has to be characterized as relational aesthetics in terms of process-oriented perception and that this leads to an Aesthetics Without Objects (AWO) approach. The relational nature of processes means that they do not happen inter-, that is, between ontologically delimited and stable entities, but rather they correspond between relations. I will try to show that AWO matches well with the onto-phenomenological-epistemic and relational models proposed by recent theories in different fields of science, especially in the relational interpretation of quantum physics. The field of aesthetics, then, does not indicate perceptual fixed contents—either subjective or objectual properties—rather it emerges from a correspondence occurring in an engaged and situated perceptual movement, an agencing that is prior to any sharp distinction between a perceiver and a perceived. I propose to call haptic this perceptual agencing. In the first section, I describe the reasons according to which the adoption of AWO seems more correct and advisable, both with respect to contemporary scientific models and to the current ecological changes on the planet. In the second section, I portray some characteristics of AWO. In the third section, I argue that AWO calls for haptic perception. In the fourth section, I briefly draw some meta-aesthetics consequences concerning, on the one side, socio-political issues of AWO and, on the other side, the possibility for a theory in an anti-foundational model. I conclude with a proposal: a process-oriented aesthetics approach has to be understood mainly as an art of thinking. This means rethinking and re-evaluating the idea of aesthetics as an artisan thought.
Anti-Diabetic Activity of Bioactive Compound Extracted from Spondias mangifera Fruit: In-Vitro and Molecular Docking Approaches
Authors: Khalid Mohammad , Alqarni Mohammed H. , Alsayari Abdulrhman , Foudah Ahmed I. , Aljarba Tariq M. , Mukim Mohammad , Alamri Mubarak A. , Abullais Shahabe Saquib , Wahab Shadma
Published: February 2022

Spondias mangifera is a drupaceous fruit popular for its flavour and health advantages. There is little scientific knowledge about S. mangifera, despite its widespread usage in traditional medicine, in the North-Eastern region of India. Inhibiting the key carbohydrate hydrolysing enzymes is one of the strategies for managing diabetes. Therefore, this study studied the antioxidant and anti-diabetic properties of different fraction S. mangifera fruit extract (SMFFs) from Indian geographical origin by in vitro experimental assays and silico docking simulation studies. The ADMET prediction for active substances was also investigated using the AdmetSAR database. Based on the binding affinity/molecular interactions between phytocompounds and target enzymes, in silico investigations were done to confirm the in vitro enzymatic inhibitory capability. β-sitosterol in EtOH-F was analysed using RP-HPLC with RP-C18 column as stationary phase and photo diode array detector. The percentage of β-sitosterol was found to be 1.21% ± 0.17% of total weight of extract (w/w). S. mangifera fruit ethanolic extract had a significant inhibitory concentration of 50% against free radicals produced by ABTS (89.71 ± 2.73%) and lipid peroxidation assay (88.26 ± 2.17%) tests. Similarly, the in vitro antidiabetic test findings indicated that S. mangifera inhibited alpha-amylase (73.42 ± 2.01%) and alpha-glucosidase (79.23 ± 1.98%) enzymes dose-dependently. The maximum glycosylated Hb percentage inhibitory activity shown in the ethanolic fraction was (83.97 ± 2.88%) at 500 µg/mL. The glucose uptake of the ethanolic fraction by the yeast cell showed significant (p < 0.05) at 500 µg/mL when compared with metformin (91.37 ± 1.59%), whereas the other fraction did not show the uptake of glucose by the yeast cell at the same concentration. In the docking study, the main phytoconstituents of S. mangifera fruit, such as oleanolic acid, beta-sitosterol, and beta amyrin, show strong affinity for pancreatic α-amylase. These results imply that S. mangifera has α-amylase and α-glucosidase inhibitory properties and may be used as antidiabetic with antioxidant characteristics.
Are Methionine Sulfoxide-Containing Proteins Related to Seed Longevity? A Case Study of Arabidopsis thaliana Dry Mature Seeds Using Cyanogen Bromide Attack and Two-Dimensional-Diagonal Electrophoresis
Authors: Kalemba Ewa Marzena , Valot Benoît , Job Dominique , Bailly Christophe , Meimoun Patrice
Published: February 2022

In recent years, several reports pointed out the role of protein oxidation in seed longevity, notably regarding the oxidation of methionine (Met) residues to methionine sulfoxide (MetO) in proteins. To further consider this question, we present a handy proteomic method based on the use of two-dimensional diagonal electrophoresis (2Dd) and cyanogen bromide (CNBr) cleavage, which we refer to as 2Dd-CNBr. CNBr treatment of proteins causes the non-enzymatic hydrolysis of peptide bonds on the carboxyl side of reduced Met residues. However, Met oxidation causes a lack of cleavage, thus modifying the electrophoretic mobility of CNBr-induced peptides. This approach was first validated using bovine serum albumin as a model protein, which confirmed the possibility of distinguishing between oxidized and non-oxidized forms of Met-containing peptides in gels. Then, the 2Dd-CNBr method was applied to the Arabidopsis thaliana seed protein extract in a control (non-oxidized) condition and in an oxidized one (as obtained following hypochlorous acid treatment). Twenty-four oxidized Met residues in 19 proteins identified by mass spectrometry were found to be surface exposed in these proteins. In the three-dimensional environment of the oxidized Met, we detected amino acid residues that could be converted by oxidation (carbonylation) or by phosphorylation, suggesting a possible interplay between Met oxidation and the other protein modifications. The identification of the proteins oxidatively modified in Met residues revealed the finding that MetO-containing proteins are related to seed longevity. Based on these results, we suggest that the method presently described also has the potential for wider applications.
Metabolomic Fingerprinting and Molecular Characterization of the Rock Samphire Germplasm Collection from the Balkan Botanic Garden of Kroussia, Northern Greece
Authors: Kadoglidou Kalliopi , Irakli Maria , Boutsika Anastasia , Mellidou Ifigeneia , Maninis Nikolas , Sarrou Eirini , Georgiadou Vasiliki , Tourvas Nikolaos , Krigas Nikos , Moysiadis Theodoros , Grigoriadou Katerina , Maloupa Eleni , Xanthopoulou Aliki , Ganopoulos Ioannis
Published: February 2022

The traditionally edible aerial parts of rock samphire (Crithmum maritimum L.) could be a valuable functional food or feed ingredient due to their high antioxidant capacity, ascorbic acid content, and rich content in secondary metabolites such as phenolics and flavonoids. The first objective of this study was to evaluate eighteen genotypes derived from different regions of Greece regarding the phytochemical contents of their soluble extracts in total phenolics, total flavonoids, and individual polyphenols as determined by LC-MS analysis, as well as ascorbic acid content and their antioxidant capacity as determined by different assays, including ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity), and FRAP (ferric reducing antioxidant power) assays. The second objective of the study was the molecular characterization of native Greek C. maritimum genotypes. Great variation among genotypes was observed in terms of the antioxidant capacity, ascorbic acid content, and phenolic compounds (total phenolic content and total flavonoid content), as well as in caffeolquinic acids and flavonoids. The principal component analysis highlighted genotypes with a higher potential in antioxidants and polyphenolics. The most promising genotypes were G9 from Kefalonia, followed by G4 from Ikaria, where both clearly exhibited a similar response with high values of evaluated traits. The molecular characterization of genotypes revealed low variability and low to moderate genetic diversity between populations. Our data indicated that the rock samphire germplasm collection from the Balkan Botanic Garden of Kroussia could serve as an important source of documented genetic material and, thus, it is suggested for further investigation to provide insight regarding cultivation and agro-processing aspects, artificial selection, or plant breeding aimed at developing C. maritimum genotypes of high-bioactive value.
Fabrication and Characterization of Hydrophobic Cellulose Nanofibrils/Silica Nanocomposites with Hexadecyltrimethoxysilane
Authors: Kim Gi-Hong , Kang Dong-Ho , Jung Bich-Nam , Shim Jin-Kie
Published: February 2022

Cellulose nanofibrils (CNFs) have attracted much attention because of their renewability and potential biocompatibility. However, CNFs are extremely hydrophilic due to the presence of a large number of hydroxyl groups, limiting their use as a water-resistant material. In this work, we controlled the adsorption behavior of silica nanoparticles on the surface of CNFs by adjusting the synthesis conditions. The silica nanoparticle size and packing efficiency on the CNF surface could be controlled by varying the ammonium hydroxide and water concentrations. In addition, hexadecyltrimethoxysilane (HDTMS) was successfully grafted onto CNF or CNF/silica nanocomposite surfaces, and the quantitative content of organic/inorganic substances in HDTMS was analyzed through XPS and TGA. The HDTMS-modified CNF/silica nanocomposites were more advantageous in terms of hydrophobicity than the HDTMS-modified CNF composites. This is because the silica nanoparticles were adsorbed on the surface of the CNFs, increasing the surface roughness and simultaneously increasing the amount of HDTMS. As a result, the HDTMS-modified CNFs showed a water contact angle (WCA) of ~80°, whereas HDTMS-modified CNF/silica nanocomposites obtained superhydrophobicity, with a WCA of up to ~159°. This study can provide a reference for the expansion of recyclable eco-friendly coating materials via the adsorption of silica nanoparticles and hydrophobic modification of CNF materials.
Effectiveness and Productivity Improvement of Conventional Pultrusion Processes
Authors: Barkanov Evgeny , Akishin Pavel , Namsone-Sile Endija
Published: February 2022

Pultrusion is a technological process in which fibers impregnated with resin move through the heated die and solidify into a composite profile with a constant cross section, as in the metallic die. The effectiveness and productivity of conventional pultrusion processes, preserving the quality of pultruded profiles, could be improved by process optimization or by the application of new, effective heating sources instead of electrical resistances with high heat losses. Due to the large dimension of the numerical problem and multiple iterations applied for the solution of government equations, an optimization methodology was developed, using the method of experimental design and the response surface technique. To develop microwave-assisted pultrusion processes, as well as pultrusion tooling design and process control, new effective electromagnetic-thermo-chemical finite element models and algorithms were developed by using general-purpose finite element software that results in considerable savings in development time and costs and makes available various modeling features of the finite element packages. The effectiveness and productivity of the optimized conventional pultrusion processes and the developed microwave-assisted pultrusion processes are estimated in comparison with the real pultrusion processes used in laboratory and industrial shops.
A Brief Review of Poly (Butylene Succinate) (PBS) and Its Main Copolymers: Synthesis, Blends, Composites, Biodegradability, and Applications
Authors: Aliotta Laura , Seggiani Maurizia , Lazzeri Andrea , Gigante Vito , Cinelli Patrizia
Published: February 2022

PBS, an acronym for poly (butylene succinate), is an aliphatic polyester that is attracting increasing attention due to the possibility of bio-based production, as well as its balanced properties, enhanced processability, and excellent biodegradability. This brief review has the aim to provide the status concerning the synthesis, production, thermal, morphological and mechanical properties underlying biodegradation ability, and major applications of PBS and its principal copolymers.
Utilizing Biomass-Based Graphene Oxide–Polyaniline–Ag Electrodes in Microbial Fuel Cells to Boost Energy Generation and Heavy Metal Removal
Authors: Yaqoob Asim Ali , Serrà Albert , Bhawani Showkat Ahmad , Ibrahim Mohamad Nasir Mohamad , Khan Anish , Alorfi Hajer S. , Asiri Abdullah M. , Hussein Mahmoud Ali , Khan Imran , Umar Khalid
Published: February 2022

Although regarded as environmentally stable, bioelectrochemical fuel cells or, microbial fuel cells (MFCs) continue to face challenges with sustaining electron transport. In response, we examined the performance of two graphene composite-based anode electrodes—graphene oxide (GO) and GO–polymer–metal oxide (GO–PANI–Ag)—prepared from biomass and used in MFCs. Over 7 days of operation, GO energy efficiency peaked at 1.022 mW/m2 and GO–PANI–Ag at 2.09 mW/m2. We also tested how well the MFCs could remove heavy metal ions from synthetic wastewater, a secondary application of MFCs that offers considerable benefits. Overall, GO–PANI–Ag had a higher removal rate than GO, with 78.10% removal of Pb(II) and 80.25% removal of Cd(II). Material characterizations, electrochemical testing, and microbial testing conducted to validate the anodes performance confirmed that using new materials as electrodes in MFCs can be an attractive approach to improve the electron transportation. When used with a natural organic substrate (e.g., sugar cane juice), they also present fewer challenges. We also optimized different parameters to confirm the efficiency of the MFCs under various operating conditions. Considering those results, we discuss some lingering challenges and potential possibilities for MFCs.
AGNet: An Attention-Based Graph Network for Point Cloud Classification and Segmentation
Authors: Jing Weipeng , Zhang Wenjun , Li Linhui , Di Donglin , Chen Guangsheng , Wang Jian
Published: February 2022

Classification and segmentation of point clouds have attracted increasing attention in recent years. On the one hand, it is difficult to extract local features with geometric information. On the other hand, how to select more important features correctly also brings challenges to the research. Therefore, the main challenge in classifying and segmenting the point clouds is how to locate the attentional region. To tackle this challenge, we propose a graph-based neural network with an attention pooling strategy (AGNet). In particular, local feature information can be extracted by constructing a topological structure. Compared to existing methods, AGNet can better extract the spatial information with different distances, and the attentional pooling strategy is capable of selecting the most important features of the topological structure. Therefore, our model can aggregate more information to better represent different point cloud features. We conducted extensive experiments on challenging benchmark datasets including ModelNet40 for object classification, as well as ShapeNet Part and S3DIS for segmentation. Both the quantitative and qualitative experiments demonstrated a consistent advantage for the tasks of point set classification and segmentation.
Continual Learning Objective for Analyzing Complex Knowledge Representations
Authors: Khan Asad Mansoor , Hassan Taimur , Akram Muhammad Usman , Alghamdi Norah Saleh , Werghi Naoufel
Published: February 2022

Human beings tend to incrementally learn from the rapidly changing environment without comprising or forgetting the already learned representations. Although deep learning also has the potential to mimic such human behaviors to some extent, it suffers from catastrophic forgetting due to which its performance on already learned tasks drastically decreases while learning about newer knowledge. Many researchers have proposed promising solutions to eliminate such catastrophic forgetting during the knowledge distillation process. However, to our best knowledge, there is no literature available to date that exploits the complex relationships between these solutions and utilizes them for the effective learning that spans over multiple datasets and even multiple domains. In this paper, we propose a continual learning objective that encompasses mutual distillation loss to understand such complex relationships and allows deep learning models to effectively retain the prior knowledge while adapting to the new classes, new datasets, and even new applications. The proposed objective was rigorously tested on nine publicly available, multi-vendor, and multimodal datasets that span over three applications, and it achieved the top-1 accuracy of 0.9863% and an F1-score of 0.9930.
Translational Applications of Wearable Sensors in Education: Implementation and Efficacy
Authors: Ferrier Brendon , Lee Jim , Mbuli Alex , James Daniel A.
Published: February 2022

Background: Adding new approaches to teaching curriculums can be both expensive and complex to learn. The aim of this research was to gain insight into students’ literacy and confidence in learning sports science with new wearable technologies, specifically a novel program known as STEMfit. Methods: A three-phase design was carried out, with 36 students participating and exposed to wearable devices and associated software. This was to determine whether the technology hardware (phase one) and associated software (phase two) were used in a positive way that demonstrated user confidence. Results: Hardware included choosing a scalable wearable device that worked in conjunction with familiar and readily available software (Microsoft Excel) that extracted data through VBA coding. This allowed for students to experience and provide survey feedback on the usability and confidence gained when interacting with the STEMfit program. Outcomes indicated strong acceptance of the program, with high levels of motivation, resulting in a positive uptake of wearable technology as a teaching tool by students. The initial finding of this study offers an opportunity to further test the STEMfit program on other student cohorts as well as testing the scalability of the system into other year groups at the university level.
Exploration-Based SLAM (e-SLAM) for the Indoor Mobile Robot Using Lidar
Authors: Ismail Hasan , Roy Rohit , Sheu Long-Jye , Chieng Wei-Hua , Tang Li-Chuan
Published: February 2022

This paper attempts to uncover one possible method for the IMR (indoor mobile robot) to perform indoor exploration associated with SLAM (simultaneous localization and mapping) using LiDAR. Specifically, the IMR is required to construct a map when it has landed on an unexplored floor of a building. We had implemented the e-SLAM (exploration-based SLAM) using the coordinate transformation and the navigation prediction techniques to achieve that purpose in the engineering school building which consists of many 100-m2 labs, corridors, elevator waiting space and the lobby. We first derive the LiDAR mesh for the orthogonal walls and filter out the static furniture and dynamic humans in the same space as the IMR. Then, we define the LiDAR pose frame including the translation and rotation from the orthogonal walls. According to the MSC (most significant corner) obtained from the intersection of the orthogonal walls, we calculate the displacement of the IMR. The orientation of the IMR is calculated from the alignment of orthogonal walls in the consecutive LiDAR pose frames, which is also assisted by the LQE (linear quadratic estimation) method. All the computation can be done in a single processor machine in real-time. The e-SLAM technique leads to a potential for the in-house service robot to start operation without having pre-scan LiDAR maps, which can save the installation time of the service robot. In this study, we use only the LiDAR and compared our result with the IMU to verify the consistency between the two navigation sensors in the experiments. The scenario of the experiment consists of rooms, corridors, elevators, and the lobby, which is common to most office buildings.
The Adiaphorization and Dehumanization of Migrations from the Ethical and Moral Approach of Public Policies
Authors: Cloquell-Lozano Alexis , Novella-García Carlos
Published: February 2022

Today, migration has become one of the main challenges for humanity. In the last ten years, the percentage of displaced people gradually increased, mainly from the southern hemisphere to the north, in search for better living conditions and, in many cases, forced by risks and instability in their countries of origin. The COVID-19 pandemic, far from minimizing migrations, further exacerbated these processes. In light of this situation, some states passed immigration laws that protect their citizens on one hand, but which violate the fundamental rights of migrants on the other. This article aims to determine the main reasons behind the alarming process of migrant dehumanization. We will verify how these national and supranational laws and decisions represent a move towards securitization through a strategic or adiaphoric process, and how ethics and morals are not at the core of these policies. With these decisions, the suffering and death of migrants generates attitudes of indifference among citizens, who often accept and normalize its occurrence. We suggest revising these public policies on migration from the framework of the ethics of Levinasian alterity and Kantian hospitality.
Export of Dissolved Organic Carbon from the Source Region of Yangtze River in the Tibetan Plateau
Authors: You Xiaoni , Li Xiangying , Sillanpää Mika , Wang Rong , Wu Chengyong , Xu Qiangqiang
Published: February 2022

The carbon release and transport in rivers are expected to increase in a warming climate with enhanced melting. We present a continuous dataset of DOC in the river, precipitation, and groundwater, including air temperature, discharge, and precipitation in the source region of the Yangtze River (SRYR). Our study shows that the average concentrations of DOC in the three end-members are characterized as the sequence of groundwater > precipitation > river, which is related to the water volume, cycle period, and river flow speed. The seasonality of DOC in the river is observed as the obvious bimodal structure at Tuotuohe (TTH) and Zhimenda (ZMD) gauging stations. The highest concentration appears in July (2.4 mg L−1 at TTH and 2.1 mg L−1 at ZMD) and the secondary high value (2.2 mg L−1 at TTH 1.9 mg L−1 at ZMD) emerges from August to September. It is estimated that 459 and 6751 tons of DOC are transported by the river at TTH and ZMD, respectively. Although the wet deposition flux of DOC is nearly ten times higher than the river flux, riverine DOC still primarily originates from soil erosion of the basin rather than precipitation settlement. Riverine DOC fluxes are positively correlated with discharge, suggesting DOC fluxes are likely to increase in the future. Our findings highlight that permafrost degradation and glacier retreat have a great effect on DOC concentration in rivers and may become increasingly important for regional biogeochemical cycles.
Correction: Tõnisson et al. Knowledge Transfer with Citizen Science: Luft-Leipzig Case Study. Sustainability 2021, 13, 7855
Authors: Tõnisson Liina , Voigtländer Jens , Weger Michael , Assmann Denise , Käthner Ralf , Heinold Bernd , Macke Andreas
Published: February 2022

The authors would like to make the following corrections about the published paper [...]