bluuebunny/crossref_metadata_2025 huggingface dataset:
Unconditionally Secure Quantum Signatures
Authors: Amiri Ryan , Andersson Erika
Published: August 2015

Signature schemes, proposed in 1976 by Diffie and Hellman, have become ubiquitous across modern communications. They allow for the exchange of messages from one sender to multiple recipients, with the guarantees that messages cannot be forged or tampered with and that messages also can be forwarded from one recipient to another without compromising their validity. Signatures are different from, but no less important than encryption, which ensures the privacy of a message. Commonly used signature protocols—signatures based on the Rivest–Adleman–Shamir (RSA) algorithm, the digital signature algorithm (DSA), and the elliptic curve digital signature algorithm (ECDSA)—are only computationally secure, similar to public key encryption methods. In fact, since these rely on the difficulty of finding discrete logarithms or factoring large primes, it is known that they will become completely insecure with the emergence of quantum computers. We may therefore see a shift towards signature protocols that will remain secure even in a post-quantum world. Ideally, such schemes would provide unconditional or information-theoretic security. In this paper, we aim to provide an accessible and comprehensive review of existing unconditionally securesecure signature schemes for signing classical messages, with a focus on unconditionally secure quantum signature schemes.
Redundancy Determination of HVDC MMC Modules
Authors: Kim Chanki , Lee Seongdoo
Published: August 2015

An availability and a reliability prediction has been made for a high-voltage direct-current (HVDC) module of VSC (Voltage Source Converter) containing DC/DC converter, gate driver, capacitor and insulated gate bipolar transistors (IGBT). This prediction was made using published failure rates for the electronic equipment. The purpose of this prediction is to determinate the additional module redundancy of VSC and the used method is “binomial failure method”.
Colloidal Stability & Conformational Changes in β-Lactoglobulin: Unfolding to Self-Assembly
Authors: Blake Steven , Amin Samiul , Qi Wei , Majumdar Madhabi , Lewis E.
Published: August 2015

A detailed understanding of the mechanism of unfolding, aggregation, and associated rheological changes is developed in this study for β-Lactoglobulin at different pH values through concomitant measurements utilizing dynamic light scattering (DLS), optical microrheology, Raman spectroscopy, and differential scanning calorimetry (DSC). The diffusion interaction parameter kD emerges as an accurate predictor of colloidal stability for this protein consistent with observed aggregation trends and rheology. Drastic aggregation and gelation were observed at pH 5.5. Under this condition, the protein’s secondary and tertiary structures changed simultaneously. At higher pH (7.0 and 8.5), oligomerizaton with no gel formation occurred. For these solutions, tertiary structure and secondary structure transitions were sequential. The low frequency Raman data, which is a good indicator of hydrogen bonding and structuring in water, has been shown to exhibit a strong correlation with the rheological evolution with temperature. This study has, for the first time, demonstrated that this low frequency Raman data, in conjunction with the DSC endotherm, can be been utilized to deconvolve protein unfolding and aggregation/gelation. These findings can have important implications for the development of protein-based biotherapeutics, where the formulation viscosity, aggregation, and stability strongly affects efficacy or in foods where protein structuring is critical for functional and sensory performance.
BAMBI Promotes C2C12 Myogenic Differentiation by Enhancing Wnt/β-Catenin Signaling
Authors: Zhang Qiangling , Shi Xin-E , Song Chengchuang , Sun Shiduo , Yang Gongshe , Li Xiao
Published: August 2015

Bone morphogenic protein and activin membrane-bound inhibitor (BAMBI) is regarded as an essential regulator of cell proliferation and differentiation that represses transforming growth factor-β and enhances Wnt/β-catenin signaling in various cell types. However, its role in skeletal muscle remains largely unknown. In the current study, we found that the expression level of BAMBI peaked in the early differentiation phase of the C2C12 rodent myoblast cell line. Knockdown of BAMBI via siRNA inhibited C2C12 differentiation, indicated by repressed MyoD, MyoG, and MyHC expression as well as reductions in the differentiation and fusion indices. BAMBI knockdown reduced the activity of Wnt/β-catenin signaling, as characterized by the decreased nuclear translocation of β-catenin and the lowered transcription of Axin2, which is a well-documented target gene of the Wnt/β-catenin signaling pathway. Furthermore, treatment with LiCl, an activator of Wnt/β-catenin signaling, rescued the reduction in C2C12 differentiation caused by BAMBI siRNA. Taken together, our data suggest that BAMBI is required for normal C2C12 differentiation, and that its role in myogenesis is mediated by the Wnt/β-catenin pathway.
Hepatitis B Virus Infection, MicroRNAs and Liver Disease
Authors: Sarkar Neelakshi , Chakravarty Runu
Published: August 2015

Hepatitis B virus (HBV) attacks the liver and can cause both acute as well as chronic liver diseases which might lead to liver cirrhosis and hepatocellular carcinoma. Regardless of the availability of a vaccine and numerous treatment options, HBV is a major cause of morbidity and mortality across the world. Recently,microRNAs (miRNAs) have emerged as important modulators of gene function. Studies on the role of miRNA in the regulation of hepatitis B virus gene expression have been the focus of modern antiviral research. miRNAs can regulate viral replication and pathogenesis in a number of different ways, which includefacilitation, direct or indirect inhibition, activation of immune response, epigenetic modulation, etc. Nevertheless, these mechanisms can appropriately be used with a diagnosticand/or therapeutic approach. The present review is an attempt to classify specific miRNAs that are reported to be associated with various aspects of hepatitis B biology, in order to precisely present the participation of individual miRNAs in multiple aspects relating to HBV.
Ovine Hair Follicle Stem Cells Derived from Single Vibrissae Reconstitute Haired Skin
Authors: Zhang Huishan , Zhang Shoubing , Zhao Huashan , Qiao Jingqiao , Liu Shuang , Deng Zhili , Lei Xiaohua , Ning Lina , Cao Yujing , Zhao Yong , Duan Enkui
Published: August 2015

Hair follicle stem cells (HFSCs) possess fascinating self-renewal capacity and multipotency, which play important roles in mammalian hair growth and skin wound repair. Although HFSCs from other mammalian species have been obtained, the characteristics of ovine HFSCs, as well as the methods to isolate them have not been well addressed. Here, we report an efficient strategy to obtain multipotent ovine HFSCs. Through microdissection and organ culture, we obtained keratinocytes that grew from the bulge area of vibrissa hair follicles, and even abundant keratinocytes were harvested from a single hair follicle. These bulge-derived keratinocytes are highly positive for Krt15, Krt14, Tp63, Krt19 and Itga6; in addition to their strong proliferation abilities in vitro, these keratinocytes formed new epidermis, hair follicles and sebaceous glands in skin reconstitution experiments, showing that these are HFSCs from the bulge outer root sheath. Taken together, we developed an efficient in vitro system to enrich ovine HFSCs, providing enough HFSCs for the investigations about the ovine hair cycle, aiming to promote wool production in the future.
Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
Authors: Sato Masahiro , Koriyama Miyu , Watanabe Satoshi , Ohtsuka Masato , Sakurai Takayuki , Inada Emi , Saitoh Issei , Nakamura Shingo , Miyoshi Kazuchika
Published: August 2015

Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of the above system is required. As a proof-of-concept, we attempted to disrupt a gene (GGTA1) encoding the α-1,3-galactosyltransferase that synthesizes the α-Gal epitope using parthenogenetically activated porcine oocytes. The lack of α-Gal epitope expression can be monitored by staining with fluorescently labeled isolectin BS-I-B4 (IB4), which binds specifically to the α-Gal epitope. When oocytes were injected with guide RNA specific to GGTA1 together with enhanced green fluorescent protein (EGFP) and human Cas9 mRNAs, 65% (24/37) of the developing blastocysts exhibited green fluorescence, although almost all (96%, 23/24) showed a mosaic fluorescent pattern. Staining with IB4 revealed that the green fluorescent area often had a reduced binding activity to IB4. Of the 16 samples tested, six (five fluorescent and one non-fluorescent blastocysts) had indel mutations, suggesting a correlation between EGFP expression and mutation induction. Furthermore, it is suggested that zygote microinjection of mRNAs might lead to the production of piglets with cells harboring various mutation types.
A Pilot Study of the Photoprotective Effects of Strawberry-Based Cosmetic Formulations on Human Dermal Fibroblasts
Authors: Gasparrini Massimiliano , Forbes-Hernandez Tamara , Afrin Sadia , Alvarez-Suarez José , Gonzàlez-Paramàs Ana , Santos-Buelga Celestino , Bompadre Stefano , Quiles José , Mezzetti Bruno , Giampieri Francesca
Published: August 2015

Strawberry polyphenols have been extensively studied over the last two decades for their beneficial properties. Recently, their possible use in ameliorating skin conditions has also been proposed; however, their role in preventing UVA-induced damage in cosmetic formulation has not yet been investigated. Skin is constantly exposed to several environmental stressors, such as UVA radiation, that induce oxidative stress, inflammation and cell death via the production of reactive oxygen species (ROS). In the present study, we assessed the potential photoprotective capacity of different strawberry-based formulations, enriched with nanoparticles of Coenzyme Q10 and with sun protection factor 10 (SPF10), in human dermal fibroblasts (HuDe) exposed to UVA radiation. We confirmed that strawberries are a very rich source of polyphenols, anthocyanins and vitamins, and possess high total antioxidant capacity. We also showed that strawberry extracts (25 μg/mL–1 mg/mL) exert a noticeable photoprotection in HuDe, increasing cell viability in a dose-dependent way, and that these effects are potentiated by the presence of CoQ10red (100 μg/mL). We have demonstrated for the first time that the topical use of strawberry extract may provide good photoprotection, even if more in-depth studies are strongly encouraged in order to evaluate the cellular and molecular effects of strawberry protection.
Recommender System for E-Learning Based on Semantic Relatedness of Concepts
Authors: Ye Mao , Tang Zhi , Xu Jianbo , Jin Lifeng
Published: August 2015

Digital publishing resources contain a lot of useful and authoritative knowledge. It may be necessary to reorganize the resources by concepts and recommend the related concepts for e-learning. A recommender system is presented in this paper based on the semantic relatedness of concepts computed by texts from digital publishing resources. Firstly, concepts are extracted from encyclopedias. Information in digital publishing resources is then reorganized by concepts. Secondly, concept vectors are generated by skip-gram model and semantic relatedness between concepts is measured according to the concept vectors. As a result, the related concepts and associated information can be recommended to users by the semantic relatedness for learning or reading. History data or users’ preferences data are not needed for recommendation in a specific domain. The technique may not be language-specific. The method shows potential usability for e-learning in a specific domain.
Insect Cuticular Hydrocarbons as Dynamic Traits in Sexual Communication
Authors: Ingleby Fiona
Published: August 2015

Recent research has demonstrated extensive within-species variation in pheromone expression in insect species, contrary to the view that pheromones are largely invariant within species. In fact, many studies on insect cuticular hydrocarbons (CHCs) show that pheromones can be highly dynamic traits that can express significant short-term plasticity across both abiotic and social environments. It is likely that this variability in CHC expression contributes to their important role in sexual signaling and mate choice. In this review, I discuss CHC plasticity and how this might influence sexual communication. I also highlight two important avenues for future research: examining plasticity in how individuals respond to CHC signals, and testing how sexual communication varies across abiotic and social environments.
The Dynamics, Ecological Variability and Estimated Carbon Stocks of Mangroves in Mahajamba Bay, Madagascar
Authors: Jones Trevor , Ratsimba Harifidy , Ravaoarinorotsihoarana Lalao , Glass Leah , Benson Lisa , Teoh Marianne , Carro Aude , Cripps Garth , Giri Chandra , Gandhi Samir , Andriamahenina Zo , Rakotomanana Rado , Roy Pierre-Francois
Published: August 2015

Mangroves are found throughout the tropics, providing critical ecosystem goods and services to coastal communities and supporting rich biodiversity. Globally, mangroves are being rapidly degraded and deforested at rates exceeding loss in many tropical inland forests. Madagascar contains around 2% of the global distribution, >20% of which has been deforested since 1990, primarily from over-harvest for forest products and conversion for agriculture and aquaculture. While historically not prominent, mangrove loss in Madagascar’s Mahajamba Bay is increasing. Here, we focus on Mahajamba Bay, presenting long-term dynamics calculated using United States Geological Survey (USGS) national-level mangrove maps contextualized with socio-economic research and ground observations, and the results of contemporary (circa 2011) mapping of dominant mangrove types. The analysis of the USGS data indicated 1050 hectares (3.8%) lost from 2000 to 2010, which socio-economic research suggests is increasingly driven by commercial timber extraction. Contemporary mapping results permitted stratified sampling based on spectrally distinct and ecologically meaningful mangrove types, allowing for the first-ever vegetation carbon stock estimates for Mahajamba Bay. The overall mean carbon stock across all mangrove classes was estimated to be 100.97 ± 10.49 Mg C ha−1. High stature closed-canopy mangroves had the highest average carbon stock estimate (i.e., 166.82 ± 15.28 Mg C ha−1). These estimates are comparable to other published values in Madagascar and elsewhere in the Western Indian Ocean and demonstrate the ecological variability of Mahajamba Bay’s mangroves and their value towards climate change mitigation.
Assessment of Above-Ground Biomass of Borneo Forests through a New Data-Fusion Approach Combining Two Pan-Tropical Biomass Maps
Authors: Langner Andreas , Achard Frédéric , Vancutsem Christelle , Pekel Jean-Francois , Simonetti Dario , Grassi Giacomo , Kitayama Kanehiro , Nakayama Mikiyasu
Published: August 2015

This study investigates how two existing pan-tropical above-ground biomass (AGB) maps (Saatchi 2011, Baccini 2012) can be combined to derive forest ecosystem specific carbon estimates. Several data-fusion models which combine these AGB maps according to their local correlations with independent datasets such as the spectral bands of SPOT VEGETATION imagery are analyzed. Indeed these spectral bands convey information about vegetation type and structure which can be related to biomass values. Our study area is the island of Borneo. The data-fusion models are evaluated against a reference AGB map available for two forest concessions in Sabah. The highest accuracy was achieved by a model which combines the AGB maps according to the mean of the local correlation coefficients calculated over different kernel sizes. Combining the resulting AGB map with a new Borneo land cover map (whose overall accuracy has been estimated at 86.5%) leads to average AGB estimates of 279.8 t/ha and 233.1 t/ha for forests and degraded forests respectively. Lowland dipterocarp and mangrove forests have the highest and lowest AGB values (305.8 t/ha and 136.5 t/ha respectively). The AGB of all natural forests amounts to 10.8 Gt mainly stemming from lowland dipterocarp (66.4%), upper dipterocarp (10.9%) and peat swamp forests (10.2%). Degraded forests account for another 2.1 Gt of AGB. One main advantage of our approach is that, once the best fitting data-fusion model is selected, no further AGB reference dataset is required for implementing the data-fusion process. Furthermore, the local harmonization of AGB datasets leads to more spatially precise maps. This approach can easily be extended to other areas in Southeast Asia which are dominated by lowland dipterocarp forest, and can be repeated when newer or more accurate AGB maps become available.
Preclinical in vivo Performance of Novel Biodegradable, Electrospun Poly(lactic acid) and Poly(lactic-co-glycolic acid) Nanocomposites: A Review
Authors: Holderegger Claudia , Schmidlin Patrick , Weber Franz , Mohn Dirk
Published: August 2015

Bone substitute materials have witnessed tremendous development over the past decades and autogenous bone may still be considered the gold standard for many clinicians and clinical approaches in order to rebuild and restore bone defects. However, a plethora of novel xenogenic and synthetic bone substitute materials have been introduced in recent years in the field of bone regeneration. As the development of bone is actually a calcification process within a collagen fiber arrangement, the use of scaffolds in the formation of fibers may offer some advantages, along with additional handling characteristics. This review focuses on material characteristics and degradation behavior of electrospun biodegradable polyester scaffolds. Furthermore, we concentrated on the preclinical in vivo performance with regard to bone regeneration in preclinical studies. The major findings are as follows: Scaffold composition and architecture determine its biological behavior and degradation characteristics; The incorporation of inorganic substances and/or organic substances within composite scaffolds enhances new bone formation; L-poly(lactic acid) and poly(lactic-co-glycolic acid) composite scaffolds, especially when combined with basic substances like hydroxyapatite, tricalcium phosphate or demineralized bone powder, seem not to induce inflammatory tissue reactions in vivo.
Core-Shell-Structured Copolyaniline-Coated Polymeric Nanoparticle Suspension and Its Viscoelastic Response under Various Electric Fields
Authors: Moon Il-Jae , Choi Hyoung
Published: August 2015

Semi-conducting poly(n-methylaniline) (PNMA)-coated poly(methyl methacrylate) (PMMA) composite nanoparticles were synthesized using cross-linked and grafted PMMA particles as a core, and then, the PNMA shell was coated via chemical oxidative polymerization on the surface of modified PMMA nanoparticles. Their electroresponsive electrorheological characteristics when dispersed in silicone were confirmed under applied electric fields using a rotational rheometer, focusing on their viscoelastic response. Using a frequency sweep test, the frequency dependence of both the storage and loss moduli was confirmed to increase upon increasing the electric field, with a stable plateau regime over the entire angular frequency range.
Fucoxanthinol, Metabolite of Fucoxanthin, Improves Obesity-Induced Inflammation in Adipocyte Cells
Authors: Maeda Hayato , Kanno Shogo , Kodate Mei , Hosokawa Masashi , Miyashita Kazuo
Published: August 2015

Fucoxanthin (Fx) is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fx metabolite into fucoxanthinol (FxOH), attenuates the weight gain of white adipose tissue of diabetic/obese KK-Ay mice. In this study, to evaluate anti-diabetic effects of Fx, we investigated improving the effect of insulin resistance on the diabetic model of KK-Ay mice. Furthermore, preventing the effect of FxOH on low-grade chronic inflammation related to oxidative stress was evaluated on 3T3-L1 adipocyte cells and a RAW264.7 macrophage cell co-culture system. A diet containing 0.1% Fx was fed to diabetic model KK-Ay mice for three weeks, then glucose tolerance was observed. Fx diet significantly improved glucose tolerance compared with the control diet group. In in vitro studies, FxOH showed suppressed tumor necrosis factor-α (TNF-α), and monocyte chemotactic protein-1 (MCP-1) mRNA expression and protein levels in a co-culture of adipocyte and macrophage cells. These findings suggest that Fx ameliorates glucose tolerance in the diabetic model mice. Furthermore, FxOH, a metabolite of Fx, suppresses low-grade chronic inflammation in adipocyte cells.
Comparative Immunogenicity of a Cytotoxic T Cell Epitope Delivered by Penetratin and TAT Cell Penetrating Peptides
Authors: Brooks Nicole , Esparon Sandra , Pouniotis Dodie , Pietersz Geoffrey
Published: August 2015

Cell penetrating peptides (CPP), including the TAT peptide from the human immunodeficiency virus transactivator of transcription (HIV-TAT) protein and penetratin from Drosophila Antennapedia homeodomain protein, translocate various cargos including peptides and proteins across cellular barriers. This mode of delivery has been harnessed by our group and others to deliver antigenic proteins or peptides into the cytoplasm of antigen processing cells (APC) such as monocyte-derived dendritic cells (MoDC). Antigens or T cell epitopes delivered by CPP into APC in vivo generate antigen-specific cytotoxic T cell and helper T cell responses in mice. Furthermore, mice immunised with these peptides or proteins are protected from a tumour challenge. The functional properties of CPP are dependent on the various cargos being delivered and the target cell type. Despite several studies demonstrating superior immunogenicity of TAT and Antp-based immunogens, none has compared the immunogenicity of antigens delivered by TAT and Antp CPP. In the current study we demonstrate that a cytotoxic T cell epitope from the mucin 1 (MUC1) tumour associated antigen, when delivered by TAT or Antp, generates identical immune responses in mice resulting in specific MUC1 T cell responses as measured by in vivo CTL assays, IFNγ ELISpot assays and prophylactic tumour protection.
Synthesis and Herbicidal Activity of New Hydrazide and Hydrazonoyl Derivatives
Authors: Šeršeň František , Gregáň Fridrich , Peško Matúš , Dvoranová Dana , Kráľová Katarína , Matkovičová Zuzana , Gregáň Juraj , Donovalová Jana
Published: August 2015

Three new hydrazide and five new hydrazonoyl derivatives were synthesized. The chemical structures of these compounds were confirmed by 1H-NMR, IR spectroscopy and elemental analysis. The prepared compounds were tested for their activity to inhibit photosynthetic electron transport in spinach chloroplasts and growth of the green algae Chlorella vulgaris. IC50 values of these compounds varied in wide range, from a strong to no inhibitory effect. EPR spectroscopy showed that the active compounds interfered with intermediates Z•/D•, which are localized on the donor side of photosystem II. Fluorescence spectroscopy suggested that the mechanism of inhibitory action of the prepared compounds possibly involves interactions with aromatic amino acids present in photosynthetic proteins.
Graphene-Based Nanomaterials as Efficient Peroxidase Mimetic Catalysts for Biosensing Applications: An Overview
Authors: Garg Bhaskar , Bisht Tanuja , Ling Yong-Chien
Published: August 2015

“Artificial enzymes”, a term coined by Breslow for enzyme mimics is an exciting and promising branch of biomimetic chemistry aiming to imitate the general and essential principles of natural enzymes using a variety of alternative materials including heterogeneous catalysts. Peroxidase enzymes represent a large family of oxidoreductases that typically catalyze biological reactions with high substrate affinity and specificity under relatively mild conditions and thus offer a wide range of practical applications in many areas of science. The increasing understanding of general principles as well as intrinsic drawbacks such as low operational stability, high cost, difficulty in purification and storage, and sensitivity of catalytic activity towards atmospheric conditions of peroxidases has triggered a dynamic field in nanotechnology, biochemical, and material science that aims at joining the better of three worlds by combining the concept adapted from nature with the processability of catalytically active graphene-based nanomaterials (G-NMs) as excellent peroxidase mimetic catalysts. This comprehensive review discusses an up-to-date synthesis, kinetics, mechanisms, and biosensing applications of a variety of G-NMs that have been explored as promising catalysts to mimic natural peroxidases.
Synthesis of Radiation Curable Palm Oil–Based Epoxy Acrylate: NMR and FTIR Spectroscopic Investigations
Authors: Salih Ashraf , Ahmad Mansor , Ibrahim Nor , Dahlan Khairul , Tajau Rida , Mahmood Mohd , Yunus Wan
Published: August 2015

Over the past few decades, there has been an increasing demand for bio-based polymers and resins in industrial applications, due to their potential lower cost and environmental impact compared with petroleum-based counterparts. The present research concerns the synthesis of epoxidized palm oil acrylate (EPOLA) from an epoxidized palm oil product (EPOP) as environmentally friendly material. EPOP was acrylated by acrylic acid via a ring opening reaction. The kinetics of the acrylation reaction were monitored throughout the reaction course and the acid value of the reaction mixture reached 10 mg KOH/g after 16 h, indicating the consumption of the acrylic acid. The obtained epoxy acrylate was investigated intensively by means of FTIR and NMR spectroscopy, and the results revealed that the ring opening reaction was completed successfully with an acrylation yield about 82%. The UV free radical polymerization of EPOLA was carried out using two types of photoinitiators. The radiation curing behavior was determined by following the conversion of the acrylate groups. The cross-linking density and the hardness of the cured EPOLA films were measured to evaluate the effect of the photoinitiator on the solid film characteristics, besides, the thermal and mechanical properties were also evaluated.
The Pattern of Fatty Acids Displaced by EPA and DHA Following 12 Months Supplementation Varies between Blood Cell and Plasma Fractions
Authors: Walker Celia , West Annette , Browning Lucy , Madden Jackie , Gambell Joanna , Jebb Susan , Calder Philip
Published: August 2015

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased in plasma lipids and blood cell membranes in response to supplementation. Whilst arachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids (FA) is less well described and there may be site-specific differences. In response to 12 months EPA + DHA supplementation in doses equivalent to 0–4 portions of oily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to identify important FA changes for plasma phosphatidylcholine (PC), cholesteryl ester (CE) and triglyceride (TAG) and for blood mononuclear cells (MNC), red blood cells (RBC) and platelets (PLAT). Dose-dependent increases in EPA + DHA were matched by decreases in several n-6 polyunsaturated fatty acids (PUFA) in PC, CE, RBC and PLAT, but were predominantly compensated for by oleic acid in TAG. Changes were observed for all FA classes in MNC. Consequently the n-6:n-3 PUFA ratio was reduced in a dose-dependent manner in all pools after 12 months (37%–64% of placebo in the four portions group). We conclude that the profile of the FA decreased in exchange for the increase in EPA + DHA following supplementation differs by FA pool with implications for understanding the impact of n-3 PUFA on blood lipid and blood cell biology.
Using Short Dietary Questions to Develop Indicators of Dietary Behaviour for Use in Surveys Exploring Attitudinal and/or Behavioural Aspects of Dietary Choices
Authors: Daly Alison , Pollard Christina , Kerr Deborah , Binns Colin , Phillips Michael
Published: August 2015

For countries where nutrition surveys are infrequent, there is a need to have some measure of healthful eating to plan and evaluate interventions. This study shows how it is possible to develop healthful eating indicators based on dietary guidelines from a cross sectional population survey. Adults 18 to 64 years answered questions about the type and amount of foods eaten the previous day, including fruit, vegetables, cereals, dairy, fish or meat and fluids. Scores were based on serves and types of food according to an established method. Factor analysis indicated two factors, confirmed by structural equation modeling: a recommended food healthful eating indicator (RF_HEI) and a discretionary food healthful eating indicator (DF_HEI). Both yield mean scores similar to an established dietary index validated against nutrient intake. Significant associations for the RF_HEI were education, income, ability to save, and attitude toward diet; and for the DF_HEI, gender, not living alone, living in a socially disadvantaged area, and attitude toward diet. The results confirm that short dietary questions can be used to develop healthful eating indicators against dietary recommendations. This will enable the exploration of dietary behaviours for “at risk” groups, such as those with excess weight, leading to more relevant interventions for populations.
Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice
Authors: Wan Xiao , Bi Jingcheng , Gao Xuejin , Tian Feng , Wang Xinying , Li Ning , Li Jieshou
Published: August 2015

Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.
Modification of Docosahexaenoic Acid Composition of Milk from Nursing Women Who Received Alpha Linolenic Acid from Chia Oil during Gestation and Nursing
Authors: Valenzuela Rodrigo , Bascuñán Karla , Chamorro Rodrigo , Barrera Cynthia , Sandoval Jorge , Puigrredon Claudia , Parraguez Gloria , Orellana Paula , Gonzalez Valeria , Valenzuela Alfonso
Published: August 2015

α-Linolenic acid (ALA) is the precursor of docosahexaenoic acid (DHA) in humans, which is fundamental for brain and visual function. Western diet provides low ALA and DHA, which is reflected in low DHA in maternal milk. Chia oil extracted from chia (Salvia hispanica L.), a plant native to some Latin American countries, is high in ALA (up to 60%) and thereby is an alternative to provide ALA with the aim to reduce DHA deficits. We evaluated the modification of the fatty acid profile of milk obtained from Chilean mothers who received chia oil during gestation and nursing. Forty healthy pregnant women (22–35 years old) tabulated for food consumption, were randomly separated into two groups: a control group with normal feeding (n = 21) and a chia group (n = 19), which received 16 mL chia oil daily from the third trimester of pregnancy until the first six months of nursing. The fatty acid profile of erythrocyte phospholipids, measured at six months of pregnancy, at time of delivery and at six months of nursing, and the fatty acid profile of the milk collected during the first six months of nursing were assessed by gas-chromatography. The chia group, compared to the control group, showed (i) a significant increase in ALA ingestion and a significant reduction of linoleic acid (LA) ingestion, no showing modification of arachidonic acid (AA), eicosapentaenoic acid (EPA) and DHA; (ii) a significant increase of erythrocyte ALA and EPA and a reduction of LA. AA and DHA were not modified; (iii) a increased milk content of ALA during the six months of nursing, whereas LA showed a decrease. AA and EPA were not modified, however DHA increased only during the first three months of nursing. Consumption of chia oil during the last trimester of pregnancy and the first three months of nursing transiently increases the milk content of DHA.
Relative Efficiency of ALS and InSAR for Biomass Estimation in a Tanzanian Rainforest
Authors: Hansen Endre , Gobakken Terje , Solberg Svein , Kangas Annika , Ene Liviu , Mauya Ernest , Næsset Erik
Published: August 2015

Forest inventories based on field sample surveys, supported by auxiliary remotely sensed data, have the potential to provide transparent and confident estimates of forest carbon stocks required in climate change mitigation schemes such as the REDD+ mechanism. The field plot size is of importance for the precision of carbon stock estimates, and better information of the relationship between plot size and precision can be useful in designing future inventories. Precision estimates of forest biomass estimates developed from 30 concentric field plots with sizes of 700, 900, …, 1900 m2, sampled in a Tanzanian rainforest, were assessed in a model-based inference framework. Remotely sensed data from airborne laser scanning (ALS) and interferometric synthetic aperture radio detection and ranging (InSAR) were used as auxiliary information. The findings indicate that larger field plots are relatively more efficient for inventories supported by remotely sensed ALS and InSAR data. A simulation showed that a pure field-based inventory would have to comprise 3.5–6.0 times as many observations for plot sizes of 700–1900 m2 to achieve the same precision as an inventory supported by ALS data.
Temporal Dependency of Yield and Quality Estimation through Spectral Vegetation Indices in Pear Orchards
Authors: Van Beek Jonathan , Tits Laurent , Somers Ben , Deckers Tom , Verjans Wim , Bylemans Dany , Janssens Pieter , Coppin Pol
Published: August 2015

Yield and quality estimations provide vital information to fruit growers, yet require accurate monitoring throughout the growing season. To this end, the temporal dependency of fruit yield and quality estimations through spectral vegetation indices was investigated in irrigated and rainfed pear orchards. Both orchards were monitored throughout three consecutive growing seasons, including spectral measurements (i.e., hyperspectral canopy reflectance measurements) as well as yield determination (i.e., total yield and number of fruits per tree) and quality assessment (i.e., fruit firmness, total soluble solids and fruit color). The results illustrated a clear association between spectral vegetation indices and both fruit yield and fruit quality (|r| > 0.75; p < 0.001). However, the correlations between vegetation indices and production variables varied throughout the growing season, depending on the phenological stage of fruit development. In the irrigated orchard, index values showed a strong association with production variables near time of harvest (|r| > 0.6; p < 0.001), while in the rainfed orchard, index values acquired during vegetative growth periods presented stronger correlations with fruit parameters (|r| > 0.6; p < 0.001). The improved planning of remote sensing missions during (rainfed orchards) and after (irrigated orchards) vegetative growth periods could enable growers to more accurately predict production outcomes and improve the production process.
An Arduino-Based Resonant Cradle Design with Infant Cries Recognition
Authors: Chao Chun-Tang , Wang Chia-Wei , Chiou Juing-Shian , Wang Chi-Jo
Published: August 2015

This paper proposes a resonant electric cradle design with infant cries recognition, employing an Arduino UNO as the core processor. For most commercially available electric cradles, the drive motor is closely combined with the bearing on the top, resulting in a lot of energy consumption. In this proposal, a ball bearing design was adopted and the driving force is under the cradle to increase the distance from the object to fulcrum and torque. The sensors are designed to detect the oscillation state, and then the force is driven at the critical time to achieve the maximum output response while saving energy according to the principle of resonance. As for the driving forces, the winding power and motors are carefully placed under the cradle. The sensors, including the three-axis accelerometer and infrared sensor, are tested and applied under swinging amplitude control. In addition, infant cry recognition technology was incorporated in the design to further develop its functionality, which is a rare feature in this kind of hardware. The proposed nonlinear operator of fundamental frequency ( ) analysis is able to identify different types of infant cries. In conclusion, this paper proposes an energy-saving electric cradle with infant cries recognition and the experimental results demonstrate the effectiveness of this approach.
Ethical Analysis for Evaluating Sustainable Business Decisions: The Case of Environmental Impact Evaluation in the Inambari Hydropower Project
Authors: Rode Julian , Le Menestrel Marc , Van Wassenhove Luk , Simon Anthony
Published: August 2015

We propose an ethical analysis as a method to reflect on how companies’ decisions promote sustainable development. The method proceeds by first identifying the choice according to financial business interests, and by then scrutinizing this choice according to consequentialist and deontological ethics. The paper applies the method to the choice of an Environmental Impact Assessment (EIA) that a consortium of Brazilian companies (EGASUR) delivered as part of their project proposal for the realization of the Inambari hydropower dam in the Peruvian Amazon. We show that if an EIA is chosen based on the attempt to maximize the financial bottom line, it raises ethical issues both from a consequentialist perspective by involving negative consequences for various stakeholder groups, and from a deontological perspective by not complying with relevant rules, guidelines, and principles. The two ethical perspectives hence reveal where the consortium faces impediments to a genuine commitment to sustainability. Building on stakeholder interviews, observations of the project developments, and the executive summary of the actual EIA, we provide indications that EGASUR has indeed made a choice that resembles a decision based on financial interests.
Integrable (2 + 1)-Dimensional Spin Models with Self-Consistent Potentials
Authors: Myrzakulov Ratbay , Mamyrbekova Galya , Nugmanova Gulgassyl , Lakshmanan Muthusamy
Published: August 2015

Integrable spin systems possess interesting geometrical and gauge invariance properties and have important applications in applied magnetism and nanophysics. They are also intimately connected to the nonlinear Schrödinger family of equations. In this paper, we identify three different integrable spin systems in (2 + 1) dimensions by introducing the interaction of the spin field with more than one scalar potential, or vector potential, or both. We also obtain the associated Lax pairs. We discuss various interesting reductions in (2 + 1) and (1 + 1) dimensions. We also deduce the equivalent nonlinear Schrödinger family of equations, including the (2 + 1)-dimensional version of nonlinear Schrödinger–Hirota–Maxwell–Bloch equations, along with their Lax pairs.
Bäcklund Transformations for Integrable Geometric Curve Flows
Authors: Qu Changzheng , Han Jingwei , Kang Jing
Published: August 2015

We study the Bäcklund transformations of integrable geometric curve flows in certain geometries. These curve flows include the KdV and Camassa-Holm flows in the two-dimensional centro-equiaffine geometry, the mKdV and modified Camassa-Holm flows in the two-dimensional Euclidean geometry, the Schrödinger and extended Harry-Dym flows in the three-dimensional Euclidean geometry and the Sawada-Kotera flow in the affine geometry, etc. Using the fact that two different curves in a given geometry are governed by the same integrable equation, we obtain Bäcklund transformations relating to these two integrable geometric flows. Some special solutions of the integrable systems are used to obtain the explicit Bäcklund transformations.
Whole-Genome Analysis of a Novel Fish Reovirus (MsReV) Discloses Aquareovirus Genomic Structure Relationship with Host in Saline Environments
Authors: Chen Zhong-Yuan , Gao Xiao-Chan , Zhang Qi-Ya
Published: August 2015

Aquareoviruses are serious pathogens of aquatic animals. Here, genome characterization and functional gene analysis of a novel aquareovirus, largemouth bass Micropterus salmoides reovirus (MsReV), was described. It comprises 11 dsRNA segments (S1–S11) covering 24,024 bp, and encodes 12 putative proteins including the inclusion forming-related protein NS87 and the fusion-associated small transmembrane (FAST) protein NS22. The function of NS22 was confirmed by expression in fish cells. Subsequently, MsReV was compared with two representative aquareoviruses, saltwater fish turbot Scophthalmus maximus reovirus (SMReV) and freshwater fish grass carp reovirus strain 109 (GCReV-109). MsReV NS87 and NS22 genes have the same structure and function with those of SMReV, whereas GCReV-109 is either missing the coiled-coil region in NS79 or the gene-encoding NS22. Significant similarities are also revealed among equivalent genome segments between MsReV and SMReV, but a difference is found between MsReV and GCReV-109. Furthermore, phylogenetic analysis showed that 13 aquareoviruses could be divided into freshwater and saline environments subgroups, and MsReV was closely related to SMReV in saline environments. Consequently, these viruses from hosts in saline environments have more genomic structural similarities than the viruses from hosts in freshwater. This is the first study of the relationships between aquareovirus genomic structure and their host environments.
Papillomavirus Infectious Pathways: A Comparison of Systems
Authors: Biryukov Jennifer , Meyers Craig
Published: August 2015

The HPV viral lifecycle is tightly linked to the host cell differentiation, causing difficulty in growing virions in culture. A system that bypasses the need for differentiating epithelium has allowed for generation of recombinant particles, such as virus-like particles (VLPs), pseudovirions (PsV), and quasivirions (QV). Much of the research looking at the HPV life cycle, infectivity, and structure has been generated utilizing recombinant particles. While recombinant particles have proven to be invaluable, allowing for a rapid progression of the HPV field, there are some significant differences between recombinant particles and native virions and very few comparative studies using native virions to confirm results are done. This review serves to address the conflicting data in the HPV field regarding native virions and recombinant particles.
How Firm and Market Characteristics Affect Profitability
Authors: Katsampoxakis Ioannis , Basdekis Haralampos , Anathreptakis Konstantinos
Published: August 2015

This study aims to assess the impact of specific corporate and market features on the profitability of firms. More precisely, the variables examined for the purposes of this study are firms' size, financial leverage, accruals, volatility of profitability, growth rate of the Greek economy, the 10-year Greek government bond yield, and the Greek sovereign debt crisis. The empirical results exhibit an average profitability of 10.71%, which varies significantly both between firms and during the time period examined. Another finding of this study is the verification of the theoretical relationship between the above variables and Greek firms' profitability between 2004 and 2012. Whereas variables such as firms' size, volatility of profitability and accruals do not seem to affect firms' profitability in a statistically significant way, the signs of the coefficients are consistent with those found the literature review.
Implementation and Visualization of Conceptual Graphs in CharGer
Authors: Delugach Harry S.
Published: August 2015

Practitioners using conceptual graphs are naturally interested in tools that support its features. The CharGer editing software has been available for many years, giving the community a chance to evaluate and contribute to CharGer's ever-expanding capabilities. As result of that experience, the author discusses a few lessons learned about visualization and implementation of conceptual graphs. Features such as actors, glossary integration, standards-based interoperability and automatic layout offer opportunities to study and improve the usefulness of conceptual graphs tools.
Language Exercise Generation
Authors: Malafeev Alexey
Published: August 2015

This manuscript presents an approach to the automatic generation of open cloze exercises based on arbitrary English text. The exercise format is similar to the open cloze test used in Cambridge English certificate exams (FCE, CAE, CPE). The presented method also makes it possible to adjust the difficulty of the resulting exercises to better suit specific proficiency levels. Three experiments were conducted to evaluate the usefulness of the machine-generated exercises, compare them with authentic Cambridge English tests and study the difficulty-setting capabilities. The experiments showed that the generation method used was quite effective. With some customization, the method can be applied to generating similar exercises for other languages.
Noise Resistant Morphological Algorithm of Moving Forklift Truck Detection on Noisy Image Data
Authors: Chernousov Vladimir Olegovich , Savchenko Andrey Vladimirovich
Published: August 2015

In this paper the authors focus on the specific problem of machine vision, namely, the video-based detection of the moving forklift truck. It is shown that the detection quality of the state-of-the-art local descriptors (SURF, SIFT, etc.) is not satisfactory if the resolution is low and the illumination is changed dramatically. The authors propose a novel algorithm to detect the presence of a cargo on the forklift truck on the basis of the mathematical morphological operators. At first, the movement direction is estimated with the updating motion history image method and the front part of the moving object is obtained. Next, contours are detected and the morphological operations in front of the moving object are used to compute several geometric features of an empty forklift. In the experimental study, it has been shown that the proposed method has 40% lower false positive rate and 27% lower false negative rate in comparison with conventional matching of local descriptors. Moreover, this algorithm is 7-35 times faster.