bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictors of Suboptimal Adherence Among Children on Antiretroviral Therapy in Southern Ethiopia: A Multicenter Retrospective Follow-Up Study
Authors: Guyo Tamirat Gezahegn , Merid Fasika , Toma Temesgen Mohammed
Published: November 2023

Objectives: Despite increased access to and availability of antiretroviral therapy, the program’s effectiveness is primarily affected by treatment adherence. Therefore, this study aimed to determine the magnitude and predictors of suboptimal adherence among children on ART in Southern Ethiopia.

Methods: A multicenter retrospective study was conducted among human immunodeficiency virus (HIV) infected children in Gamo and South Omo zone public health facilities. To identify factors associated with suboptimal adherence, a binary logistic regression model was fitted. Variables with a p-value ≤0.25 in bivariable logistic regression analysis were included in multivariable logistic regression analysis. p-value <0.05 was used to declare statistical significance.

Results: The suboptimal adherence was determined to be 30.3% (95% CI: 25.5%, 35.6%). Advanced clinical stage, hemoglobin level <10 mg/dL, unchanged initial regimen, and non-disclosure of HIV sero-status were significant predictors of suboptimal adherence.

Conclusion: Suboptimal adherence is a significant public health problem in the study setting. Therefore, designing interventions towards improving adherence is needed especially for children with poor clinical characteristics.
Trustworthy Digital Representations of Analog Information—An Application-Guided Analysis of a Fundamental Theoretical Problem in Digital Twinning
Authors: Boche Holger , Böck Yannik N. , Mönich Ullrich J. , Fitzek Frank H. P.
Published: November 2023

This article compares two methods of algorithmically processing bandlimited time-continuous signals in light of the general problem of finding “suitable” representations of analog information on digital hardware. Albeit abstract, we argue that this problem is fundamental in digital twinning, a signal-processing paradigm the upcoming 6G communication-technology standard relies on heavily. Using computable analysis, we formalize a general framework of machine-readable descriptions for representing analytic objects on Turing machines. Subsequently, we apply this framework to sampling and interpolation theory, providing a thoroughly formalized method for digitally processing the information carried by bandlimited analog signals. We investigate discrete-time descriptions, which form the implicit quasi-standard in digital signal processing, and establish continuous-time descriptions that take the signal’s continuous-time behavior into account. Motivated by an exemplary application of digital twinning, we analyze a textbook model of digital communication systems accordingly. We show that technologically fundamental properties, such as a signal’s (Banach-space) norm, can be computed from continuous-time, but not from discrete-time descriptions of the signal. Given the high trustworthiness requirements within 6G, e.g., employed software must satisfy assessment criteria in a provable manner, we conclude that the problem of “trustworthy” digital representations of analog information is indeed essential to near-future information technology.
The Impact of Rye and Barley Malt and Different Strains of Saccharomyces cerevisiae on Beer Volatilome
Authors: Tocci Noemi , Riccio Gian Marco , Ramu Ganesan Abirami , Hoellrigl Philipp , Robatscher Peter , Conterno Lorenza
Published: November 2023

Craft breweries are continuously searching for beers made with locally produced raw materials and unique flavor profiles to respond to consumer requests. We explored the behavior of three commercial strains of Saccharomyces cerevisiae in the fermentation of ale beer with a high prevalence of rye malt in comparison to pure barley malt. In total, 34 volatile organic compounds were identified, with esters and alcohols being the quantitatively most abundant classes. The yeast strain appeared to impart more differences in the beer’s volatile profile compared to malt. In particular, S. cerevisiae var. diastaticus Y2 strain was associated with a higher production of esters, while strain S. cerevisiae Y3 was correlated to the higher amounts of terpenes together with the lowest relative abundance of volatile acids. Our findings encourage further investigation of the fermentation performance of several yeast strains to produce beers with unique flavors.
Risk Factors for Long COVID in Older Adults
Authors: Hu Yunguang , Liu Yifan , Zheng Huiwen , Liu Longding
Published: November 2023

As time has passed following the COVID-19 pandemic, individuals infected with SARS-CoV-2 have gradually exhibited a variety of symptoms associated with long COVID in the postacute phase of infection. Simultaneously, in many countries worldwide, the process of population aging has been accelerating. Within this context, the elderly population has not only become susceptible and high-risk during the acute phase of COVID-19 but also has considerable risks when confronting long COVID. Elderly individuals possess specific immunological backgrounds, and during the process of aging, their immune systems can enter a state known as “immunosenescence”. This further exacerbates “inflammaging” and the development of various comorbidities in elderly individuals, rendering them more susceptible to long COVID. Additionally, long COVID can inflict both physical and mental harm upon elderly people, thereby reducing their overall quality of life. Consequently, the impact of long COVID on elderly people should not be underestimated. This review seeks to summarize the infection characteristics and intrinsic factors of older adults during the COVID-19 pandemic, with a focus on the physical and mental impact of long COVID. Additionally, it aims to explore potential strategies to mitigate the risk of long COVID or other emerging infectious diseases among older adults in the future.
The Past and Future of Psychiatric Sleep Research
Authors: Pollmächer Thomas
Published: November 2023

Sleep studies in psychiatric disorders date back to the first half of the 20th century. So far, success in establishing disease-specific sleep-related biomarkers has been quite limited. This is particularly obvious regarding insomnia, where there is no reliable correlation between subjective complaints and physiological measures of sleep. Finally, it must be acknowledged that the physiology of sleep and wakefulness and their subjective perception are essentially independent dimensions. Still, however, these dimensions are mixed up in clinical practice and research. This creates confusion, can be harmful for patients, and is identified as a major obstacle for successful psychiatric sleep research. It is proposed here that future sleep research should treat physiological sleep as a variable in psychiatric disorders which, independently of patient perception, has a transdiagnostic value, as was already proposed a decade ago by the Research Domain Criteria.
Two-Layer Cooperative Optimization of Flexible Interconnected Distribution Networks Considering Electric Vehicle User Satisfaction Degree
Authors: Wan Dai , Mo Wenhui , Li Junhao , Yang Chunzhi , Wu Jinbo , Zhou Qianfan , Gong Yusheng
Published: November 2023

The scaled access of electric vehicles (EVs) exacerbates load fluctuations in distribution networks, which is not conducive to the stable and economic operation of the distribution networks. At present, user satisfaction degree is generally low. To avoid this problem, this paper proposed a two-layer cooperative optimization of flexible interconnected distribution networks considering EV user satisfaction degree. First, the EV user satisfaction degree model is established by considering EV users’ charging waiting time, charging power, and other indicators. At the same time, an EV charging mode switching model is constructed based on the number of EVs entering the network and their battery charge state. On this basis, the Monte Carlo algorithm is used to generate the results of the daily distribution of EV loads taking into account the user satisfaction degree, so as to improve the load ratio of the transformer in the distribution network. Further, a two-layer cooperative optimization of flexible interconnected distribution networks considering electric vehicle user satisfaction degree is developed with the daily operating cost of each network as the optimization objective. Finally, a flexible interconnected power distribution network consisting of three power distribution networks is used as an example for validation. The results show that this method is effective in improving EV user satisfaction degree and reducing the peak-to-valley ratio of the system load while taking into account the safe and economic operation of the distribution network, which greatly improves the reliability and economy of the operation of the flexible interconnected power distribution network.
Heat and Mass Transfer Processes and Evaporation of a Liquid Droplet on a Structured Surface
Authors: Antonov Dmitrii V. , Islamova Anastasia G. , Orlova Evgeniya G.
Published: November 2023

The characteristics of water droplet heating and evaporation on structured hydrophobic and hydrophilic surfaces in the range of static contact angles from 73° to 155° were studied experimentally using high-speed video recording. Two fundamentally different technologies for applying coatings on a metal surface were used in comparison with the results on a polished surface. Microscopic studies were conducted to identify the features of the formed coatings. The wetting properties were characterized by means of the static contact angle and the contact angle hysteresis: on polished surface No. 1 (contact angle—73°, hysteresis—11°), on structured surface No. 2 (contact angle—125°, hysteresis—9°), and on structured surface No 3 (contact angle—155°, hysteresis—7°). The experimental dependences of the droplet evaporation rate on the different surfaces under normal conditions (ambient air temperature—293 K, atmospheric pressure, humidity—35%) were obtained. The evaporation regimes of droplets on the surfaces under study were identified. Water droplets evaporated in the pinning mode on surfaces No. 1 and No. 2. When a water droplet evaporated on surface No 3, the droplet was in the constant contact angle regime for ≈90% of its lifetime. Based on the experimental data obtained, a two-dimensional model of conjugate heat and mass transfer was developed, which describes the heating and evaporation of a liquid droplet on structured hydrophobic and hydrophilic surfaces at a wide range of contact angles. Satisfactory agreement was obtained between the numerical simulation results and experimental data. Using the model, the fields of temperature, concentration and other key characteristics were established at different points in time. Recommendations for its application in the development of gas–vapor–droplet applications were formulated.
Challenges of Large Converter-Fed Synchronous Machines for Variable-Speed Pumped Hydro Storage
Authors: Polster Stefan , Deschler Johannes , Renner Herwig , Bocquel Aurelie , Janssen Martin
Published: November 2023

The green energy transition of electrical energy production is leading to an increasing share of total energy production for volatile renewable energy sources, mainly wind and solar power. To handle this volatile production, flexible and efficient energy storage is required. The development of high-power converters has enabled the generation of variable-speed pumped hydro storage power plants, combining the so-far-unequalled energy storage capacity of classical pumped-storage hydro power plants and the recently increased operation requirements. The introduction of large-scale converters has led to new challenges in the overall design of power plant systems. This paper intended to take a closer look at a large-scale converter-fed synchronous generator, especially the distribution of the current and voltage harmonics caused by the converter in the implemented generation system. Thereby, holistic design considerations for an ideal loss distribution as well as possible measures to limit the effects of harmonic coupling at the generator shaft and bearings are discussed. Furthermore, basic considerations of harmonic emission to the connected network are described. These topics are addressed by analyzing on-site measurements at an 85 MVA converter-fed synchronous generator with a voltage source inverter, underpinned with the theoretical background.
Geophysical Interpretation of Horizontal Fractures in Shale Oil Reservoirs Using Rock Physical and Seismic Methods
Authors: Guo Zhiqi , Gao Wenxuan , Liu Cai
Published: November 2023

Horizontal fractures are one of the factors that significantly affect the ultimate productivity of shale oil reservoirs. However, the prediction of horizontal fractures by using seismic methods remains a challenge, which is due to the complex elastic and seismic responses that are associated with horizontal fractures. A framework that predicts horizontal fractures by seismic rock physical methods has been developed in the present study. A shale model is then proposed to quantify the shale elastic responses that are associated with the properties of the horizontal fractures. The modeling results that are based on the logging data validated the applicability of the proposed model, and the predicted fracture properties could be used to evaluate the development of horizontal fractures. According to the framework of the Poisson impedance, a horizontal fracture indicator is suggested to represent the logging-derived fracture density in terms of a combination of elastic properties. By using seismic-inverted elastic properties, the obtained indicator enabled an estimation of zones with the potential development of horizontal fractures. The established indicator showed a good correlation with the fracture density and could be used as an effective indicator in the prediction of horizontal fractures in shale oil reservoirs. Furthermore, seismic data applications show a consistency between the development of horizontal fractures and the production status of the boreholes. This result highlights the importance of horizontal fractures for the ultimate productivity and emphasizes the applicability of the proposed methods.
Changes in the Functional Role of the Tejo Estuary (Portugal, Europe) According to Fish Ecological Guilds
Authors: França Susana
Published: November 2023

Estuaries are extremely productive ecosystems, providing habitats for numerous aquatic species and crucial ecological services. The Tejo estuary, one of the largest European estuaries, has been thoroughly studied, and its important functional role as a nursery for several commercially important fish species is already established. In the present work, a trait-based approach was applied to functionally describe the fish community structure of the Tejo estuary and to enlighten potential changes in the ecosystem functioning at this level, following environmental changes expected to occur. To predict the distribution of species from the two most representative ecological guilds of the Tejo estuary, estuarine residents and marine migrants, species distribution models were built using an ensemble technique (combining forecasts of single models). The predictions obtained were more accurate for the marine migrants and the species distribution was strongly related with salinity, whereas estuarine species, were also influenced by depth, habitat type and river flow. The potential distributions of these ecological guilds showed that marine migrants will tend to use upstream areas in the estuary, where salinity is lower. Nonetheless, salinity is expected to increase as extreme weather events such as droughts tend to occur more frequently, decreasing favorable habitat availability for these species, and thus threatening the crucial role this ecosystem plays for these species.
Growth Performance, Feed Utilisation, Digestive and Metabolic Enzyme Activity, and Liver Morphohistology in Hybrid Tilapia (Oreochromis mossambicus × Oreochromis niloticus) Juveniles Fed with the Inclusion of Chitosan in Their Diet
Authors: Méndez-Martínez Yuniel , Vera-Veliz Alan Rodrigo , Cortés-Jacinto Edilmar , Cruz-Quintana Yanis , Botello-Leon Aroldo , Mendoza-Carranza Pedro Daniel , Calvo Natalia S.
Published: November 2023

This study aimed to evaluate the growth performance, feed utilisation, digestive and metabolic enzyme activity, and liver histology in juveniles of hybrid red tilapia (Oreochromis mossambicus × Oreochromis niloticus) fed with the inclusion of chitosan in their diet. Six dietary chitosan levels (0 “control’’, 10, 20, 30, 40, and 50 g kg−1) were used to feed juvenile fish (initial weight 7.50 ± 0.20 g) that were cultured for eight weeks in 18 tanks at a density of 15 fish/tank. The growth performance presented significant differences (p ˂ 0.05) for weight gain, specific growth rate, feed conversion ratio, hepatosomatic index, and survival rate. The digestive and metabolic enzyme activities were significantly (p < 0.05) influenced by the levels of chitosan in the administered diet. Histologically, no damage was found in the liver; however, morphometrically, a significant difference (p < 0.05) was found in the hepatocyte area and sinusoid area. Vacuolisation of hepatocytes was found in treatments with 40 and 50 g kg−1 of chitosan in the diet. Treatments with doses of chitosan showed a better response (p < 0.05) compared to the control treatment in most of the different groups of variables analysed. The result of the principal component analysis suggests that a diet containing 40 g kg−1 of chitosan is optimal for tilapia growth performance.
High-Pressure Pasteurization of Oat Okara
Authors: Helstad Amanda , Marefati Ali , Ahlström Cecilia , Rayner Marilyn , Purhagen Jeanette , Östbring Karolina
Published: November 2023

The issue of the short microbiological shelf life of residues from the plant-based beverage industry creates a large food waste problem. Today, the oat beverage residue, in this study referred to as oat okara, is generally converted to energy or used as animal feed. High-pressure pasteurization (200 MPa, 400 MPa, and 600 MPa) was applied to oat okara to investigate the effect on shelf life and microbiological activity. A 4-week microbiological storage study was performed and thermal properties, viscosity, and water and oil holding capacities were analyzed. The total aerobic count, including yeast and mold, was significantly reduced (p < 0.05) by 600 MPa after four weeks of storage at 4 °C. The content of lactic acid bacteria after four weeks of storage was low for untreated oat okara (3.2 log CFU/g) but, for 600 MPa, the content remained at the detection limit (2.3 log CFU/g). Conversely, the treatments of 200 MPa and 400 MPa increased the microbial content of the total aerobic count significantly (p < 0.05) after two weeks in comparison to untreated oat okara. The thermal properties of untreated and high-pressure-treated oat okara demonstrated an increase in protein denaturation of the 12S globulin, avenalin, when higher pressure was applied (400–600 MPa). This was also confirmed in the viscosity measurements where a viscosity peak for avenalin was only present for untreated and 200 MPa treated oat okara. The water holding capacity did not change as a function of high-pressure treatment (3.5–3.8 mL/g) except for the treatment at 200 MPa, which was reduced (2.7 mL/g). The oil holding capacity was constant (1.2–1.3 mL/g) after all treatments. High-pressure pasteurization of 600 MPa reduced the microbial content in oat okara resulting in a shelf life of 2–4 weeks. However, more research is required to identify the microorganisms in oat okara to achieve a microbiologically safe product that can be used for food applications.
Evaluation of Short-Term Toxicity and Cholesterol-Lowering Effects in Rats Exposed to Dietary Fiber Derived from Cassava Pulp
Authors: Posridee Kakanang , Chirinang Pornariya , Oonsivilai Anant , Oonsivilai Ratchadaporn
Published: November 2023

The dietary fiber extracted from cassava pulp, composed of crude fiber, neutral detergent fiber (NDF), and cellulose content, demonstrates promise as a functional food ingredient. The study’s objectives encompassed the assessment of short-term toxicity and the evaluation of its potential cholesterol-lowering effects. The results indicated that cassava pulp dietary fiber (CDF) is well-tolerated with non-toxic thresholds determined at 10.01 g/kg body weight/day for male rats and 11.21 g/kg body weight/day for female rats during the short-term toxicity assessment. Furthermore, CDF exhibited notable cholesterol-lowering effects, significantly reducing serum triglyceride and serum total cholesterol levels, along with decreased liver total lipids and liver cholesterol levels. In contrast, it led to significant increases in fecal total lipids and cholesterol when compared to the control group. Most notably, there were no significant differences in terms of serum triglyceride, serum total cholesterol, liver total lipids, and liver cholesterol between CDF and the conventional cholesterol-lowering medication, simvastatin. These findings underscore the potential of cassava pulp dietary fiber as a natural and safe alternative for managing hyperlipidemia and related conditions. It offers a valuable avenue for the development of functional foods aimed at improving cardiovascular health and further investigation for its potential application in the field of nutraceuticals.
Associations between Combined Psychological and Lifestyle Factors with Pain Intensity and/or Disability in Patients with Chronic Low Back Pain: A Cross-Sectional Study
Authors: Tsatsaraki Eirini , Bouloukaki Izolde , Kontakis Georgios , Vakis Antonis F. , Basta Maria
Published: November 2023

Chronic low back pain (CLBP) is common in primary care, causing disability and economic burden globally. We aimed to compare socio-demographic, health, lifestyle, and psychological factors in people with and without CLBP and correlate them with clinical outcomes in people with CLBP. A total of 253 volunteers with and 116 without CLBP provided sociodemographic information, daily habits, medical history, subjective sleep complaints (Penn State Sleep Questionnaire), low back pain intensity, and disability (Quebec Back Pain Disability Scale), as well as the Zung Self-Rating Scale for self-assessment of depression and Self-Rating Anxiety Scale. CLBP diagnosis was linked with female gender and older age, as well as a higher level of sleep complaints such as sleepiness, OSA and insomnia symptoms, and a higher prevalence of moderate to severe depressive symptoms. The combination of moderate to severe depressive symptoms with obstructive sleep apnea or insomnia symptoms was the most important predictive factor for functional disability in CBLP patients (OR 13.686, 95% CI 4.581–40.885; p < 0.001). In conclusion, depressive symptoms and subjective sleep complaints appear to relate to greater CLBP intensity and/or CLBP-related disability in people with CLBP. A holistic approach is crucial for treating chronic CLBP patients, including psychological and sleep issue assessment and management, to improve their quality of life.
A Survey on the Use of Outcome Measures during Physical Therapy Interventions by Physical Therapists in Korea
Authors: Lim Jae-Hyun , Kim So-Yeong , Kim Byeong-Geun
Published: November 2023

The purpose of this study was to determine the current status of patient care provided by Korean physical therapists (KPTs) in clinical practice by studying the outcome measures (OMs) used in physical therapy interventions among KPTs with experience in treating patients. A total of 225 KPTs with experience in treating patients in clinical settings participated in the study and completed the online questionnaire. The questionnaire included questions about the use of OMs and the reasons for using them, as well as the types, benefits, and barriers of OMs. The participants’ responses were analyzed and reported in terms of frequencies and percentages. A total of 220 questionnaires were analyzed. The results show that the majority of KPTs in clinical practice used OMs during interventions. The main reasons for using OMs were to check the patient’s condition and to determine the direction and effectiveness of treatment. In terms of the types of OMs used, the highest percentage of subjects used both patient-reported OMs (PROMs) and performance-based OMs (PBOMs). They chose OMs that were quick and easy to use and used them voluntarily. Barriers to and reasons for not using OMs were similar, including lack of benefits, lack of time, and problems with patient performance and uncooperative behavior. When analyzing the effect of demographic characteristics on the use of OMs, we found that physical therapists specializing in musculoskeletal and neurological systems, physical therapists with longer treatment times, and physical therapists who valued OMs were more likely to use them. Based on the results of this study, it is recommended that improvements in the work environment and healthcare system are needed to enhance the professionalism of KPTs working in the field of physical agent therapy by improving their awareness of Oms and improving the quality of physical therapy interventions.
Assessment of Nitrate in Groundwater from Diffuse Sources Considering Spatiotemporal Patterns of Hydrological Systems Using a Coupled SWAT/MODFLOW/MT3DMS Model
Authors: Correa-González Alejandra , Hernández-Bedolla Joel , Martínez-Cinco Marco Antonio , Sánchez-Quispe Sonia Tatiana , Hernández-Hernández Mario Alberto
Published: November 2023

In recent years, due to various anthropogenic activities, such as agriculture and livestock, the presence of nitrogen-associated contaminants has been increasing in surface- and groundwater resources. Among these, the main compounds present in groundwater are ammonia, nitrite, and nitrate. However, it is sometimes difficult to assess such effects given the scarcity or lack of information and the complexity of the system. In the current study, a methodology is proposed to assess nitrate in groundwater from diffuse sources considering spatiotemporal patterns of hydrological systems using a coupled SWAT/MODFLOW/MT3DMS model. The application of the model is carried out using a simplified simulation scheme of hydrological and agricultural systems because of the limited spatial and temporal data. The study area includes the Cuitzeo Lake basin in superficial flow form and the Morelia–Querendaro aquifer in groundwater flow form. The results within the methodology are surface runoff, groundwater levels, and nitrate concentrations present in surface- and groundwater systems. The results indicate that the historical and simulated nitrate concentrations were obtained within acceptable values of the statistical parameters and, therefore, are considered adequate.
Source Attribution of Atmospheric Dust Deposition to Utah Lake
Authors: Telfer Justin T. , Brown Mitchell M. , Williams Gustavious P. , Tanner Kaylee B. , Miller A. Woodruff , Sowby Robert B. , Miller Theron G.
Published: November 2023

Atmospheric deposition (AD) is a significant source of nutrient loading to waterbodies around the world. However, the sources and loading rates are poorly understood for major waterbodies and even less understood for local waterbodies. Utah Lake is a eutrophic lake located in central Utah, USA, and has high-nutrient levels. Recent research has identified AD as a significant source of nutrient loading to the lake, though contributions from dust particles make up 10% of total AD. To better understand the dust AD sources, we sampled suspected source locations and collected deposition samples around the lake. We analyzed these samples using Inductively Coupled Plasma (ICP) for 25 metals to characterize their elemental fingerprints. We then compared the lake samples to the source samples to determine likely source locations. We computed spectral angle, coefficient of determination, multi-dimensional scaling, and radar plots to characterize the similarity of the samples. We found that samples from local dust sources were more similar to dust in lake AD samples than samples from distant sources. This suggests that the major source of the dust portion of AD onto Utah Lake is the local empty fields south and west of the lake, and not the farther playa and desert sources as previously suggested. Preliminary data suggest that dust AD is associated with dry, windy conditions and is episodic in nature. We show that AD from dust particles is likely a small portion of the overall AD nutrient loading on Utah Lake, with the dry and precipitation sources contributing most of the load. This case identifies AD sources to Utah Lake and provides an example of data and methods that can be used to assess similarity or perform attribution for dust, soil, and other environmental data. While we use ICP metals, any number of features can be used with these methods if normalized.
Sperm Capacitation and Kinematics in Phodopus Hamsters
Authors: Sanchez-Rodriguez Ana , Idrovo Ingrid I. D. , Rielo Juan Antonio , Roldan Eduardo R. S.
Published: November 2023

This study was designed to analyze changes in the spermatozoa of three species of Phodopus hamsters incubated under different conditions. Cauda epididymal sperm were incubated for 4 h in modified Tyrode’s medium containing albumin, lactate, pyruvate, and Hepes (mTALP-H), in the same medium with the addition of bicarbonate (mTALP-BH), or with bicarbonate and 20 ng/mL of progesterone (mTALP-BH+P4). Media with bicarbonate are believed to promote capacitation in rodent species. Sperm motility, viability, capacitation patterns, and kinematics were assessed at different times. Capacitation in live cells was quantified after staining with Hoechst 33258 and chlortetracycline. Patterns believed to correspond to non-capacitated cells (F pattern), capacitated, acrosome-intact cells (B pattern), and acrosome-reacted cells (AR pattern) were recognized. Kinematics were examined via computer-assisted sperm analysis (CASA). The results showed a decrease in total motility in all three species in different media, with a sharp decrease in progressive motility in bicarbonate-containing media (without or with progesterone), suggesting hyperactivated motion. However, none of the other signs of hyperactivation described in rodents (i.e., decrease in STR or LIN, together with an increase in ALH) were observed. F pattern cells diminished with time in all media and were generally lower in P. roborovskii and higher in P. campbelli. B pattern cells increased in mTALP-BH media in all species. Progesterone did not enhance the percentage of B pattern cells. Finally, AR pattern cells increased in all species incubated in different media, showing the highest percentage in P. roborovskii and the lowest in P. campbelli. Comparisons between media revealed that there were higher percentages of F pattern cells and lower percentages of B pattern cells over time in medium without bicarbonate (mTALP-H) in comparison to media containing bicarbonate (mTALP-BH; mTALP-BH+P4). Overall, changes consistent with the acquisition of capacitation and development of hyperactivated motility were found; however, further studies are required to better characterize media necessary to support the pathways involved in these processes in Phodopus species.
Association of SLC12A1 and GLUR4 Ion Transporters with Neoadjuvant Chemoresistance in Luminal Locally Advanced Breast Cancer
Authors: Justo-Garrido Montserrat , López-Saavedra Alejandro , Alcaraz Nicolás , Cortés-González Carlo C. , Oñate-Ocaña Luis F. , Caro-Sánchez Claudia Haydee Sarai , Castro-Hernández Clementina , Arriaga-Canon Cristian , Díaz-Chávez José , Herrera Luis A.
Published: November 2023

Chemoresistance to standard neoadjuvant treatment commonly occurs in locally advanced breast cancer, particularly in the luminal subtype, which is hormone receptor-positive and represents the most common subtype of breast cancer associated with the worst outcomes. Identifying the genes associated with chemoresistance is crucial for understanding the underlying mechanisms and discovering effective treatments. In this study, we aimed to identify genes linked to neoadjuvant chemotherapy resistance in 62 retrospectively included patients with luminal breast cancer. Whole RNA sequencing of 12 patient biopsies revealed 269 differentially expressed genes in chemoresistant patients. We further validated eight highly correlated genes associated with resistance. Among these, solute carrier family 12 member 1 (SLC12A1) and glutamate ionotropic AMPA type subunit 4 (GRIA4), both implicated in ion transport, showed the strongest association with chemoresistance. Notably, SLC12A1 expression was downregulated, while protein levels of glutamate receptor 4 (GLUR4), encoded by GRIA4, were elevated in patients with a worse prognosis. Our results suggest a potential link between SLC12A1 gene expression and GLUR4 protein levels with chemoresistance in luminal breast cancer. In particular, GLUR4 protein could serve as a potential target for drug intervention to overcome chemoresistance.
PGT-M, a Useful Tool to Manage the Lynch Syndrome Transmission
Authors: Listorti Ilaria , Manzo Roberta , Arrivi Cristiana , Mencacci Cecilia , Biricik Anil , Greco Ermanno , Greco Pierfrancesco
Published: November 2023

Lynch syndrome is one of the most common hereditary cancer sensitivity syndromes and is caused by autosomal-dominant germline mutations in DNA mismatch repair genes. In patients affected by this syndrome, pre-implantation genetic testing for monogenic disorders (PGT-M) could be the elective technique used to prevent the transmission of this hereditary syndrome to offspring. Notably, despite the severity of the condition, some authors have observed a markedly lower demand for PGT-M in these patients compared to those with other hereditary conditions. A 34-year-old woman with a medical history of Lynch syndrome associated with endometrial cancer came to the Villa Mafalda fertility center in Rome in order to conceive a healthy baby. In a pre-implantation genetic testing for aneuploidy (PGT-A) + PGT-M cycle, eight blastocysts were formed. Six out of eight blastocysts were affected by the same mother syndrome. One of the other two was aneuploid and the other one was a mosaic embryo, which resulted in a healthy pregnancy. The aim of this report is to emphasize the importance of a multidisciplinary approach to managing patients with this condition. In vitro fertilization (IVF), specifically PGT-M, is a tool that allow patients to conceive biological children with lower risk of inheriting the disease.
First Report of aac(6′)-Ib and aac(6′)-Ib-cr Variant Genes Associated with Mutations in gyrA Encoded Fluoroquinolone Resistance in Avian Campylobacter coli Strains Collected in Tunisia
Authors: Gharbi Manel , Abbas Mohammed Abdo Saghir , Hamrouni Safa , Maaroufi Abderrazak
Published: November 2023

The aac(6′)-Ib gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant aac(6′)-Ib-cr gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 Campylobacter isolates, including 91 C. jejuni and 41 C. coli, were selected from broiler hens isolates. The aac(6′)-Ib gene was amplified using PCR and was subsequently digested with the BtsCI restriction enzyme to identify aac(6′)-Ib-cr. Among these isolates, 31 out of 41 C. coli (75.6%) and 1 (0.98%) C. jejuni were positive for the aac(6′)-Ib gene, which was identified as the aac(6′)-Ib-cr variant in 10 (32.25%) C. coli isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on Campylobacter coli strains harboring both the aac(6′)-Ib and aac(6′)-Ib-cr variants. These genes were present in Campylobacter isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments.
Comparison of Transcriptomic Changes in Survivors of Exertional Heat Illness with Malignant Hyperthermia Susceptible Patients
Authors: Chang Leon , Gardner Lois , House Carol , Daly Catherine , Allsopp Adrian , Roiz de Sa Daniel , Shaw Marie-Anne , Hopkins Philip M.
Published: November 2023

Exertional heat illness (EHI) is an occupational health hazard for athletes and military personnel–characterised by the inability to thermoregulate during exercise. The ability to thermoregulate can be studied using a standardised heat tolerance test (HTT) developed by The Institute of Naval Medicine. In this study, we investigated whole blood gene expression (at baseline, 2 h post-HTT and 24 h post-HTT) in male subjects with either a history of EHI or known susceptibility to malignant hyperthermia (MHS): a pharmacogenetic condition with similar clinical phenotype. Compared to healthy controls at baseline, 291 genes were differentially expressed in the EHI cohort, with functional enrichment in inflammatory response genes (up to a four-fold increase). In contrast, the MHS cohort featured 1019 differentially expressed genes with significant down-regulation of genes associated with oxidative phosphorylation (OXPHOS). A number of differentially expressed genes in the inflammation and OXPHOS pathways overlapped between the EHI and MHS subjects, indicating a common underlying pathophysiology. Transcriptome profiles between subjects who passed and failed the HTT (based on whether they achieved a plateau in core temperature or not, respectively) were not discernable at baseline, and HTT was shown to elevate inflammatory response gene expression across all clinical phenotypes.
Pharmacokinetics and Changes in Lipid Mediator Profiling after Consumption of Specialized Pro-Resolving Lipid-Mediator-Enriched Marine Oil in Healthy Subjects
Authors: Irún Pilar , Carrera-Lasfuentes Patricia , Sánchez-Luengo Marta , Belio Úrsula , Domper-Arnal María José , Higuera Gustavo A. , Hawkins Malena , de la Rosa Xavier , Lanas Angel
Published: November 2023

Omega-3 polyunsaturated fatty acids (PUFAs) play a vital role in human health, well-being, and the management of inflammatory diseases. Insufficient intake of omega-3 is linked to disease development. Specialized pro-resolving mediators (SPMs) are derived from omega-3 PUFAs and expedite the resolution of inflammation. They fall into categories known as resolvins, maresins, protectins, and lipoxins. The actions of SPMs in the resolution of inflammation involve restricting neutrophil infiltration, facilitating the removal of apoptotic cells and cellular debris, promoting efferocytosis and phagocytosis, counteracting the production of pro-inflammatory molecules like chemokines and cytokines, and encouraging a pro-resolving macrophage phenotype. This is an experimental pilot study in which ten healthy subjects were enrolled and received a single dose of 6 g of an oral SPM-enriched marine oil emulsion. Peripheral blood was collected at baseline, 3, 6, 9, 12, and 24 h post-administration. Temporal increases in plasma and serum SPM levels were found by using LC-MS/MS lipid profiling. Additionally, we characterized the temporal increases in omega-3 levels and established fundamental pharmacokinetics in both aforementioned matrices. These findings provide substantial evidence of the time-dependent elevation of SPMs, reinforcing the notion that oral supplementation with SPM-enriched products represents a valuable source of essential bioactive SPMs.
Time-Restricted Eating and Its Metabolic Benefits
Authors: Mishra Sneha , Persons Patress A. , Lorenzo Andrea M. , Chaliki Swarna S. , Bersoux Sophie
Published: November 2023

Newer management strategies are being evaluated to treat obesity, which continues to increase worldwide. After 12 h of fasting, the body switches from glucose to fat metabolism, regulating protein synthesis and autophagy. These cellular responses are central to the metabolic benefits of time-restricted eating (TRE), independent of calorie restriction and weight loss, and they have heightened interest in TRE regimens. Controversy remains, however, regarding the benefits of TRE regimens. We reviewed the current literature and concluded that TRE is equivalent to calorie restriction for weight loss and has positive effects for patients with diseases such as nonalcoholic fatty liver disease, cancer, and cardiovascular disease.
Sea Ice as a Factor of Primary Production in the European Arctic: Phytoplankton Size Classes and Carbon Fluxes
Authors: Kudryavtseva Elena , Kravchishina Marina , Pautova Larisa , Rusanov Igor , Glukhovets Dmitry , Shchuka Alexander , Zamyatin Ivan , Torgunova Nadezhda , Chultsova Anna , Politova Nadezhda , Savvichev Alexander
Published: November 2023

The seasonally ice-covered marine region of the European Arctic has experienced warming and sea ice loss in the last two decades. During expeditions in August 2020 and 2021, new data on size-fractioned primary production (PP), chlorophyll a concentration, phytoplankton biomass and composition and carbon fixation rates in the dark were obtained in the marginal ice zone (MIZ) of the Barents Sea, Nansen Basin and Greenland Sea to better understand the response of Arctic ecosystems to ongoing climate changes. Four different situations were observed in the study region: (i) a bloom of the large-cell diatom Podosira glacialis, whose biomass was trapped in a strong halocline at the edge of a dense ice cover; (ii) a bloom of the chain-like colonies of Thalassiosira diatoms on the shelf in mixed waters in fields of shallow ice that could be supported by “fresh” elements in the polynya condition, as well as by terrestrial run-off and drifting ices; at the late stage, this bloom was accompanied by intensive growth of Phaeocystis pouchetti; (iii) dominance of small-cell phytoplankton under weakened stratification and the significant influence of the Atlantic water, depleted of microelements and silicates; (iv) dominance of dinoflagellates of eutrophic water in the contact zone between the water masses of Arctic origin and Atlantic origin in clear water under conditions of increased light intensity. The >10 µm phytoplankton cell size group increased its relative contribution to PP as a response to stratification, light and nutrient load associated with sea ice conditions. Small phytoplankton with sizes < 2 µm formed the basis of total PP in the MIZ regardless of the state of the sea ice.
Seamlessly Coupling Hydrological Modelling Systems and GIS through Object-Oriented Programming
Authors: Xiang Xiaohua , Pan Zhijun , Wu Xiaoling , Yang Hong
Published: November 2023

Coupling hydrological modelling systems (HMS) with a geographic information system (GIS) can significantly enhance hydrological research and expand its applications. The calculation for HMS requires geographic information data; however, the current GIS data structure is not equipped to support the object-oriented hydrological modelling. Due to different objectives and design concepts, the differences between HMS and GIS have been profound, especially in their data structures from the perspective of object-oriented programming (OOP). This research introduces a novel approach to extend ArcGIS data structures for HMS, facilitating seamless coupling. This approach employs Microsoft Component Object Model (COM) technology to construct custom data structures that align with hydrological OOP principles. These can then be integrated into ArcGIS through a custom ArcGIS layer as an add-on. As a result, the HMS can leverage the full functionality of ArcGIS without the need for re-coding existing modelling systems. Moreover, HMS can be readily developed by using COM compatible computer languages, enabling the easy adaptation of this coupling approach to other HMS to ensure computational efficiency and to maximise the benefits of ArcGIS features. This new approach has been successfully implemented with the Xin’anjiang model, and the results validate its effectiveness in coastal areas.
The Mechanical Properties and Water-Reducing and Retarding Mechanism of a Xylonic Cement Admixture
Authors: Han Feng , Huang Kaijian , Wei Yang , Han Jian , Xu Yong
Published: November 2023

This study explores the mechanical properties, as well as the water-reducing and setting delay mechanism, of a novel xylonic acid-based water reducer applied to cementitious materials. Four xylonic acid water reducers were synthesized in this study: XACa (PX) from pure xylose, XACa (HS) from hemicellulose hydrolysate, XANa (PX) from pure xylose, and XANa (HS) from hemicellulose hydrolysate. These were generated through the whole-cell catalysis of Gluconobacter oxydans bacteria, using pure xylose and hemicellulose hydrolysate as substrates. The findings indicate that the xylonic acid-based water reducer can attain a water-reducing capability between 14% and 16% when the dosage (expressed as a mass fraction of cement) is roughly 0.2%. In initial and final setting tests, XACa (PX) demonstrated a pronounced retarding influence at admixture levels below 0.15%, reaching its apex at 0.10%. This delayed the initial setting time by 76% and the final setting time by 136% relative to the control group. However, a slight pro-setting effect was noted beyond a 0.2% dosage. In the compressive and flexural tests of concrete, under the same slump, the XA group improved its mechanical properties by 5% to 10% compared to the SodiuM lignosulfonate (SL) group. In the air content and chloride ion migration resistance tests, the XA group reduced the air content by 38% compared to the SL group, but also increased the data of rapid chloride migration (DRCM) by 16%. Characterization studies revealed that the carboxyl and hydroxyl groups in xylonic acid undergo chemisorption with the Si-O bonds on the surface of cement particles. These groups interact with the Si-O bonds on cement particles, contributing to water-reducing effects and delaying the setting process by impeding Ca2+ ion aggregation in the calcium-silicate-hydrate gel. Its significant water-reducing effect, adjustable setting time, and excellent mechanical and durability properties suggest its viability as an alternative to lignosulfonate series water-reducing agents.
Estimates for the Approximation and Eigenvalues of the Resolvent of a Class of Singular Operators of Parabolic Type
Authors: Muratbekov Mussakan , Muratbekov Madi , Igissinov Sabit
Published: November 2023

In this paper, we study a differential operator of parabolic type with a variable and unbounded coefficient, defined on an infinite strip. Sufficient conditions for the existence and compactness of the resolvent are established, and an estimate for the maximum regularity of solutions of the equation Lu=f∈L2(Ω) is obtained. Two-sided estimates for the distribution function of approximation numbers are obtained. As is known, estimates of approximation numbers show the rate of best approximation of the resolvent of an operator by finite-dimensional operators. The paper proves the assertion about the existence of positive eigenvalues among the eigenvalues of the given operator and finds two-sided estimates for them.