bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved Sensitivity of Allergen Detection by Immunoaffinity LC-MS/MS Using Ovalbumin as a Case Study
Authors: Röder Martin , Wiacek Claudia , Lankamp Frauke , Kreyer Jonathan , Weber Wolfgang , Ueberham Elke
Published: November 2021

Food allergies are caused by severe hypersensitivity to specific food allergens such as the egg protein ovalbumin. It is therefore important to test food products for the presence of allergens to protect allergic people from accidental ingestion. For egg detection, ELISA is the only reasonable commercially available test format, although the recognition of target allergens can be affected by food processing, which may lead to false negative results. Current mass spectrometry-based detection methods may overcome this issue, but these approaches are often less sensitive. Here we combined the advantages of antibody-based and MS-based methods by developing an immunoaffinity LC-MS/MS technique to detect the common egg allergen Gal d 2. We investigated the principal functionality of this method with incurred cookie material containing whole egg powder. We found that the new method matched easily the sensitivity of egg specific ELISA tests. Further western blot experiments indicated that this strategy may be unaffected by food processing, providing an important alternative strategy for the detection and quantification of allergens in food.
Tea and Chicory Extract Characterization, Classification and Authentication by Non-Targeted HPLC-UV-FLD Fingerprinting and Chemometrics
Authors: Pons Josep , Bedmar Àlex , Núñez Nerea , Saurina Javier , Núñez Oscar
Published: November 2021

Tea is a widely consumed drink in the world which is susceptible to undergoing adulterations to reduce manufacturing costs and rise financial benefits. The development of simple analytical methodologies to assess tea authenticity, as well as to detect and quantify frauds, is an important matter considering the rise of adulteration issues in recent years. In the present study, untargeted HPLC-UV and HPLC-FLD fingerprinting methods were employed to characterize, classify, and authenticate tea extracts belonging to different varieties (red, green, black, oolong, and white teas) by partial least squares-discriminant analysis (PLS-DA), as well as to detect and quantify adulteration frauds when chicory was used as the adulterant by partial least squares (PLS) regression, to ensure the authenticity and integrity of foodstuffs. Overall, PLS-DA showed a good classification and grouping of the tea samples according to the tea variety and, except for some white tea extracts, perfectly discriminated from the chicory ones. One hundred percent classification rates for the PLS-DA calibration models were achieved, except for green and oolong tea when HPLC-FLD fingerprints were employed, which showed classification rates of 96.43% and 95.45%, respectively. Good predictions were also accomplished, also showing, in almost all the cases, a 100% classification rate for prediction, with the exception of white tea and oolong tea when HPLC-UV fingerprints were employed that exhibited a classification rate of 77.78% and 88.89%, respectively. Good PLS results for chicory adulteration detection and quantitation were also accomplished, with calibration, cross-validation, and external validation errors beneath 1.4%, 6.4%, and 3.7%, respectively. Acceptable prediction errors (below 21.7%) were also observed, except for white tea extracts that showed higher errors which were attributed to the low sample variability available.
Evolutionary Characterization of the Short Protein SPAAR
Authors: Lee Jiwon , Wacholder Aaron , Carvunis Anne-Ruxandra
Published: November 2021

Microproteins (<100 amino acids) are receiving increasing recognition as important participants in numerous biological processes, but their evolutionary dynamics are poorly understood. SPAAR is a recently discovered microprotein that regulates muscle regeneration and angiogenesis through interactions with conserved signaling pathways. Interestingly, SPAAR does not belong to any known protein family and has known homologs exclusively among placental mammals. This lack of distant homology could be caused by challenges in homology detection of short sequences, or it could indicate a recent de novo emergence from a noncoding sequence. By integrating syntenic alignments and homology searches, we identify SPAAR orthologs in marsupials and monotremes, establishing that SPAAR has existed at least since the emergence of mammals. SPAAR shows substantial primary sequence divergence but retains a conserved protein structure. In primates, we infer two independent evolutionary events leading to the de novo origination of 5′ elongated isoforms of SPAAR from a noncoding sequence and find evidence of adaptive evolution in this extended region. Thus, SPAAR may be of ancient origin, but it appears to be experiencing continual evolutionary innovation in mammals.
Genome-Wide Identification and Analysis of the WRKY Gene Family and Cold Stress Response in Acer truncatum
Authors: Li Yan , Li Xiang , Wei Jiatong , Cai Kewei , Zhang Hongzhi , Ge Lili , Ren Zengjun , Zhao Chunli , Zhao Xiyang
Published: November 2021

WRKY transcription factors constitute one of the largest gene families in plants and are involved in many biological processes, including growth and development, physiological metabolism, and the stress response. In earlier studies, the WRKY gene family of proteins has been extensively studied and analyzed in many plant species. However, information on WRKY transcription factors in Acer truncatum has not been reported. In this study, we conducted genome-wide identification and analysis of the WRKY gene family in A. truncatum, 54 WRKY genes were unevenly located on all 13 chromosomes of A. truncatum, the highest number was found in chromosomes 5. Phylogenetic relationships, gene structure, and conserved motif identification were constructed, and the results affirmed 54 AtruWRKY genes were divided into nine subgroup groups. Tissue species analysis of AtruWRKY genes revealed which were differently exhibited upregulation in flower, leaf, root, seed and stem, and the upregulation number were 23, 14, 34, 18, and 8, respectively. In addition, the WRKY genes expression in leaf under cold stress showed that more genes were significantly expressed under 0, 6 and 12 h cold stress. The results of this study provide a new insight the regulatory function of WRKY genes under abiotic and biotic stresses.
R-CRISPR: A Deep Learning Network to Predict Off-Target Activities with Mismatch, Insertion and Deletion in CRISPR-Cas9 System
Authors: Niu Rui , Peng Jiajie , Zhang Zhipeng , Shang Xuequn
Published: November 2021

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—associated protein 9 (Cas9) system is a groundbreaking gene-editing tool, which has been widely adopted in biomedical research. However, the guide RNAs in CRISPR-Cas9 system may induce unwanted off-target activities and further affect the practical application of the technique. Most existing in silico prediction methods that focused on off-target activities possess limited predictive precision and remain to be improved. Hence, it is necessary to propose a new in silico prediction method to address this problem. In this work, a deep learning framework named R-CRISPR is presented, which devises an encoding scheme to encode gRNA-target sequences into binary matrices, a convolutional neural network as feature extractor, and a recurrent neural network to predict off-target activities with mismatch, insertion, or deletion. It is demonstrated that R-CRISPR surpasses six mainstream prediction methods with a significant improvement on mismatch-only datasets verified by GUIDE-seq. Compared with the state-of-art prediction methods, R-CRISPR also achieves competitive performance on datasets with mismatch, insertion, and deletion. Furthermore, experiments show that data concatenate could influence the quality of training data, and investigate the optimal combination of datasets.
Obstetric Complications and Polygenic Risk Score: Which Role in Predicting a Severe Short-Term Outcome in Psychosis?
Authors: Tosato Sarah , Bonetto Chiara , Vassos Evangelos , Lasalvia Antonio , De Santi Katia , Gelmetti Margherita , Cristofalo Doriana , Richards Alexander , Ruggeri Mirella ,   on behalf of the PICOS-Veneto Group
Published: November 2021

Understanding and improving the outcomes of psychosis remains a major challenge for clinical research. Obstetric complications (OCs) as a risk factor for schizophrenia (SZ) have been investigated as a potential predictor of outcomes in relation to illness severity and poorer treatment outcome, but there are less reports on first episode psychosis (FEP) patients. We test whether OCs, collected in a cohort of FEP patients, can predict illness course and psychopathology severity after 2 years from the onset. Moreover, we explore whether the SZ-polygenic risk score (PRS) would predict the illness course and whether the interaction between OCS and PRS shows a significant effect. A cohort of 264 FEP patients were assessed with standardized instruments. OCs were recorded using the Lewis–Murray scale in interviews with the patients’ mothers: 30% of them reported at least one OC. Patients with at least one OC were more likely to have a non-remitting course of illness compared to those without OCs (35.3% vs. 16.3%, p = 0.014). No association between SZ-PRS and course of illness nor evidence for a gene–environment interaction was found. In our sample, poor short-term outcomes were associated with OCs, while SZ-PRS was not a prognostic indicator of poor outcomes.
Assessing Thermal Maturity through a Multi-Proxy Approach: A Case Study from the Permian Faraghan Formation (Zagros Basin, Southwest Iran)
Authors: Spina Amalia , Cirilli Simonetta , Sorci Andrea , Schito Andrea , Clayton Geoff , Corrado Sveva , Fernandes Paulo , Galasso Francesca , Montesi Giovanni , Pereira Zelia , Rashidi Mehrab , Rettori Roberto
Published: November 2021

This study focuses on the thermal maturity of Permian deposits from the Zagros Basin, Southwest Iran, employing both optical methods (Thermal Alteration Index, Palynomorph Darkness Index, Vitrinite Reflectance, UV Fluorescence) and geochemical analyses of organic matter (Rock Eval Pyrolysis and MicroRaman spectroscopy) applied to the Faraghan Formation along two investigated Darreh Yas and Kuh e Faraghan surface sections. Furthermore, an integrated palynofacies and lithofacies analysis was carried out in order to integrate the few studies on the depositional environment. The Faraghan Formation, which is widely distributed in the Zagros area, generally consists of shale intercalated with sandstones and pebble conglomerates in the lower part, followed by a succession of sandstone, siltstone and shaly intercalations and with carbonate levels at the top. The integrated palynofacies and lithofacies data confirm a coastal depositional setting evolving upwards to a shallow marine carbonate environment upwards. Rock Eval Pyrolysis and Vitrinite Reflectance analysis showed that the organic matter from samples of the Darreh Yas and Kuh e Faraghan sections fall in the mature to postmature range with respect to the oil to gas generation window, restricting the thermal maturity range proposed by previous authors. Similar results were obtained with MicroRaman spectroscopy and optical analysis such as Thermal Alteration Index and UV Fluorescence. Palynomorph Darkness Index values were compared with Rock Eval Pyrolysis and vitrinite reflectance values and discussed for the first time in the late stage of oil generation.
Educating for a Holistic View of the Earth System: A Review
Authors: Ribeiro Tiago , Orion Nir
Published: November 2021

The global society of today struggles with grand challenges, such as climate change, the degradation of ecosystems, and the loss of bio- and geodiversity, as identified in several documents. The search for solutions to these and other problems on the way to sustainable development necessarily involves a better understanding of the Earth system and its dynamics. The Earth system is composed of five highly dependent and interrelated subsystems that exchange matter and energy. This notion is at the base of what in the literature is named Earth System Science (ESS). Humanity has been profoundly altering the dynamics of this system, leading to the proposal of a new geological epoch—the Anthropocene. Developing a holistic understanding of the complex and tangled relationships between subsystems and the role of human impacts is the target of study of Earth System Education (ESE). With the assumptions of ESS, ESE is emerging as a new approach in science education. Based on a deep knowledge of the planet and the development of specific competencies, such as system thinking, it is possible to perform more actively and consciously in the relationships that citizens develop with the Earth system, enabling the existence of a more viable future for humanity.
The Yorktown Formation: Improved Stratigraphy, Chronology, and Paleoclimate Interpretations from the U.S. Mid-Atlantic Coastal Plain
Authors: Dowsett Harry J. , Robinson Marci M. , Foley Kevin M. , Herbert Timothy D.
Published: November 2021

The Yorktown Formation records paleoclimate conditions along the mid-Atlantic Coastal Plain during the mid-Piacenzian Warm Period (3.264 to 3.025 Ma), a climate interval of the Pliocene in some ways analogous to near future climate projections. To gain insight into potential near future changes, we investigated Yorktown Formation outcrops and cores in southeastern Virginia, refining the stratigraphic framework. We analyzed 485 samples for alkenone-based sea surface temperature (SST) and productivity estimates from the Holland and Dory cores, an outcrop at Morgarts Beach, Virginia, and the lectostratotype of the Yorktown Formation at Rushmere, Virginia, and analyzed planktonic foraminferal assemblage data from the type section. Using the structure of the SST record, we improved the chronology of the Yorktown Formation by establishing the maximum age ranges of the Rushmere (3.3–3.2 Ma) and Morgarts Beach (3.2–3.15 Ma) Members. SST values for these members average ~26 °C, corroborating existing sclerochronological data. Increasing planktonic foraminifer abundance, productivity, and species diversity parallel increasing SST over the MIS M2/M1 transition. These records constitute the greatest temporal concentration of paleoecological estimates within the Yorktown Formation, aiding our understanding of western North Atlantic temperature patterns, seasonality and ocean circulation during this interval. We provide a chronologic framework for future studies analyzing ecological responses to profound climate change.
Correction: Kim et al. Effects of Nursing Simulation Using Mixed Reality: A Scoping Review. Healthcare 2021, 9, 947
Authors: Kim Kyeng-Jin , Choi Moon-Ji , Kim Kyu-Jin
Published: November 2021

The authors would like to make the following corrections about the published paper [...]
Efavirenz Is Not Associated with an Increased Risk of Depressive Disorders in Patients Living with HIV: An 11-Year Population-Based Study in Taiwan
Authors: Li Chia-Wen , Chen Yen-Chin , Lee Nan-Yao , Chen Po-Lin , Li Ming-Chi , Li Chung-Yi , Ko Wen-Chien , Ko Nai-Ying
Published: November 2021

(1) Background: This study aimed to determine the association between the use of efavirenz and depressive disorders among human immunodeficiency virus (HIV)-infected patients. (2) Methods: A retrospective cohort study was conducted using Taiwan’s National Health Insurance Database. We identified patients receiving anti-retroviral therapy (ART) between 2000 and 2009; these patients were followed until 2010 for diagnoses of depressive disorders using the Cox proportional hazard model to estimate hazard ratios. (3) Results: After up to 11 years of follow-up, the incidence of depressive disorders for the efavirenz-treated group was estimated at 12.2/1000 person-years (PYs), and the control group was at 12.5/1000 PY (p = 0.822). The independent risk factors for depressive disorders included an insurance premium of less than NTD 17,820 (New Taiwan Dollars—NTD) (adjusted hazard ratio (aHR) 2.59, 95% confidence interval (CI), 1.79–3.76, p < 0.001), and between NTD 17,821 and NTD 26,400 (aHR 1.55, 95% CI, 1.04–2.31, p = 0.030), living in Southern Taiwan (aHR 1.49, 95% CI, 1.21–1.84, p = 0.002), and with a psychiatric history (excluding depressive disorders) (aHR 4.59, 95% CI, 3.51–6.01, p = 0.030). (4) Conclusions: This study concluded that ART-treated patients with a past history of psychiatric disorders, lower insurance premium, and living in Southern Taiwan have an increased risk of depressive disorders, which are not associated with the use of efavirenz.
Hand Hygiene Knowledge and Perception among the Healthcare Workers during the COVID-19 Pandemic in Qassim, Saudi Arabia: A Cross-Sectional Survey
Authors: Abalkhail Adil , Mahmud Ilias , Alhumaydhi Fahad A. , Alslamah Thamer , Alwashmi Ameen S. S. , Vinnakota Divya , Kabir Russell
Published: November 2021

Hand hygiene is among the most important factors of infection control in healthcare settings. Healthcare workers are the primary source of hospital-acquired infection. We assessed the current state of hand hygiene knowledge, perception, and practice among the healthcare workers in Qassim, Saudi Arabia. In this cross-sectional study, we used the hand hygiene knowledge and perception questionnaire developed by the World Health Organization. Knowledge and perceptions were classified into good (80–100%), moderate (60–79%), and poor (<60% score). The majority of the healthcare workers had moderate knowledge (57.8%) and perception (73.4%) of hand hygiene. Males were less likely to have moderate/good knowledge compared to females (OR: 0.52, p < 0.05). Private healthcare workers were less likely (OR: 0.33, p < 0.01) to have moderate/good perceptions compared to the government healthcare workers. Healthcare workers who received training on hand hygiene were more likely to have good/moderate perception (OR: 3.2, p < 0.05) and to routinely use alcohol-based hand rubs (OR: 3.8, p < 0.05) than the ones without such training. Physicians are more likely (OR: 4.9, p < 0.05) to routinely use alcohol-based hand rubs than technicians. Our research highlighted gaps in hand hygiene knowledge, perception and practice among healthcare workers in Qassim, Saudi Arabia and the importance of training in this regard.
Resilience as a Mediator of the Association between Spirituality and Self-Management among Older People with Chronic Obstructive Pulmonary Disease
Authors: Chen Zhongyi , Jiang Yuyu , Chen Mengjie , Baiyila Nuerdawulieti , Nan Jiang
Published: November 2021

This study examined the mediating effect of resilience in the relationship between spirituality and self-management among older people with chronic obstructive pulmonary disease (COPD). The participants were 151 older people with COPD in four general hospitals in Jiangsu Province, China. Data were collected from September 2020 to May 2021 using a questionnaire developed by the investigator, the Function Assessment of Chronic Illness Therapy-Spiritual Scale (FACIT-SP-12), 10-item Connor-Davidson Resilience Scale (CD-RISC-10), and COPD Self-Management Scale (CSMS). One-way ANOVA and t-test were used to compare the level of self-management in patients with different sociodemographic and clinical characteristics. Partial correlation analysis was used to explore the correlation between spirituality, resilience, and self-management. Hierarchical multiple regression analyses were performed to examine the contribution of spirituality and resilience to the prediction of self-management. A bootstrapping test was implemented using the SPSS PROCESS macro to test the statistical significance of the mediating effect. There was a pairwise positive correlation between spirituality, resilience, and self-management. Resilience mediated the relationship between spirituality and self-management. These findings suggested that resilience interventions could be incorporated into future COPD self-management interventions to better improve self-management and health outcomes. Moreover, resilience should be an important component of healthy aging initiatives.
The Increasing Investment of Real Estate in the Health System—A Comparison between the USA and Europe
Authors: Bachmann Marta , Taha-Mehlitz Stephanie , Ochs Vincent , Frey Daniel. M. , Enodien Bassey , Eriksson Urs , Taha Anas
Published: November 2021

Background: This study aimed to compare property development and increasing investment in real estate by the healthcare system organizations in the USA and Europe. Real estate investments have upsurged in healthcare due to the multiple benefits to patients and medical practitioners. Methods: The approach of acquiring data was through secondary sources and online questionnaires. The researchers applied inclusion and exclusion criteria by exclusively including the articles published after 2014 to ensure the validity and reliability of the information. Results: A total of 53.33% of the articles reviewed focused on the United States, while 46.67% concentrated on Europe. The development of real estate in healthcare is essential in both regions due to the challenges faced with the current infrastructure. Study Limitation: Currently, there are very few studies concentrating on the research topic. Conclusions: The USA and Europe should focus on increasing real estate investments in healthcare by focusing on hospitals and trusts, rehabilitation centers, and nursing homes.
International Students’ Mental Health Care in China: A Systematic Review
Authors: Wu Yanping , Liu Wuqianhui , Liu Aijun , Lin-Schilstra Li , Lyu Ping
Published: November 2021

International students in China are facing difficulties while adapting their Chinese culture, and their life is influenced by the widespread of the Coronavirus Disease 2019 (COVID-19), and caring for their mental health is currently challenging. As a result, our aim is to explore the current mental health care of this minority in China and to provide useful suggestions for future research and institutes. We used the systematic review method, and it was conducted on 11 existing pieces of literature. Our results confirm the unsatisfying psychological situation of international students and the lack of research in this area. We focus on the causes and symptoms of mental problems and explore the effectiveness of methods.
Medical Students’ Perspective and Knowledge of Asymptomatic Hyperuricemia and Gout Management: A Cross-Sectional Study
Authors: Zuzic Furlan Sanja , Rusic Doris , Kumric Marko , Bozic Josko , Vilovic Marino , Vilovic Tina , Rada Marko , Cerovecki Venija , Tomicic Marion
Published: November 2021

The prevalence and incidence of gout doubled from 1990 to 2017. Therefore, we can expect that a number of doctors have come across a patient with gout in their daily practice. Hence, we wanted to investigate how familiar our medical students, as future medical professionals, are with gout. This cross-sectional survey included Medical Studies students from the two largest universities in Croatia: the University of Split School of Medicine, and the University of Zagreb School of Medicine, and included a total of 221 fifth or sixth year medical students. Most students gave correct answers to questions about treatment approach and non-pharmacological interventions in asymptomatic hyperuricemia (>80%). Less than 3% of all students agreed they knew enough about care for patients with asymptomatic hyperuricemia, whereas almost 15% thought they were well familiar with care for gout patients. Less than 8% of students considered their school education adequate on both topics, and less than 2% were aware of the existence of EULAR guidelines. Physicians lacking in the latest knowledge on the pathophysiology of gout, the influence of lifestyle, and genetic factors limits their ability to properly manage gout. With increasing prevalence, gout should be more represented in medical students’ education.
Assessments of Muscle Thickness and Tonicity of the Masseter and Sternocleidomastoid Muscles and Maximum Mouth Opening in Patients with Temporomandibular Disorder
Authors: Lee Keunhyo , Chon Seungchul
Published: November 2021

The purpose of this study was to compare the muscle thickness and tone of the masseter and sternocleidomastoid (SCM) muscles and maximum mouth opening (MMO) in subjects with and without temporomandibular disorders (TMD), and perform a correlation comparison on the results of the TMD group. Sixty patients were allocated to the TMD group (n = 30) or the non-TMD group (n = 30). Ultrasound imaging, myotonometer, and vernier calipers were used to determine the related changes in muscle thickness and muscle tone in masseter and SCM, and MMO, respectively. The TMD group revealed a significant decrease than the non-TMD group in the muscle thickness of masseter and SCM, respectively (p < 0.001), with a significant increase in frequency (p < 0.001) and stiffness (p < 0.001) in the masseter muscle tone, with a significant increase in frequency (p < 0.001) and stiffness (p = 0.005) in the SCM muscle tone, a significant decrease in the MMO (p < 0.001). There was a moderate negative correlation between the relaxed state of masseter muscle thickness and stiffness of SCM muscle tone (r = −0.40, p = 0.002), and a moderate negative correlation between the relaxed state of SCM muscle thickness and frequency of SCM muscle tone (r = −0.42, p = 0.001). There was a moderate negative correlation between the clenching state of SCM muscle thickness and the frequency of SCM muscle tone (r = −0.47, p < 0.001). In addition, a moderate negative correlation between MMO and frequency of SCM muscle tone (r = −0.44, p < 0.001). The muscle thickness was decreased, and the muscle tone was increased in the masseter and SCM muscle, respectively. Additionally, MMO was decreased in patients with TMD compared with non-TMD.
Promotion of Self-Management of Chronic Disease in Children and Teenagers: Scoping Review
Authors: Catarino Marta , Charepe Zaida , Festas Constança
Published: November 2021

Background: The scientific literature describes that self-management of chronic illness leads to improved health outcomes. Knowledge about interventions that promote self-management behaviors in children and teenagers has been poorly clarified. This study aims to map, in the scientific literature, the nature and extent of interventions that promote self-management of chronic disease, implemented and evaluated in contexts of health care provided to children and teenagers. Methods: The guidelines proposed by the Joanna Briggs Institute were followed. The survey was conducted in June 2021, with access to international databases and gray literature, in Portuguese, English, French, and Spanish. Results: Interventions that promote self-management of children and teenagers can be developed through a local contact or through technological means of support for health care. The use of online supports, such as applications or communication platforms, should be parameterized with health professionals, according to the needs of users. Conclusions: The acquisition of self-management skills in pediatrics is a process supported by the family, health professionals and the community, in which the nurse, in partnership, can promote communication and health education through cognitive strategies, behavioral programs included in physical or online programs, adjusted to the patients’ needs.
Investigating the Immediate Influence of Moderate Pedal Exercises during an Assembly Work on Performance and Workload in Healthy Men
Authors: Alhaag Mohammed H. , Ghaleb Atef M. , Mansour Lamjed , Ramadan Mohamed Z.
Published: November 2021

Physical inactivity has increased in prevalence among adults in industrialized and developing countries owing to the fact that the majority of job situations require individuals to remain seated for extended periods of time. This research aims to evaluate the influence of cycling on a stationary bike while executing a keyboard assembly task on the task completion time, error percentage, and physiological and subjective measurements. The physiological measures were electroencephalography (EEG) and electrocardiographic (ECG) signal responses, whereas the subjective measures were subjective workload ratings and subjective body discomforts. Two variables were evaluated, namely assembly methods (with versus without pedal exercises at a moderate intensity) and session testing (pre- versus post-test). Thus, the repeated measures design (i.e., assembly method by session testing of participants) was used. According to the completion time, error %, participant self-reports, and ECG and EEG statistical analysis data, the participants’ performances in the keyboard assembly task did not decrease while they performed pedaling exercises (p > 0.05). Additionally, when participants completed the assembly task while executing the pedaling exercises, the mean inter-beat (RR) intervals significantly reduced (p < 0.05) while the mean heart rate increased (p < 0.05), which mean that pedaling exercises caused physical workloads on the participants. Participant performance was unaffected by performing a workout while performing the assembly activity. Thus, administrations should encourage their employees to engage in short sessions of moderate-intensity exercise similar to the suggested exercise in the study to improve a person’s physical health during work without interfering with the effectiveness of work.
Energy and Water Related Parameters in Tomato and Cucumber Greenhouse Crops in Semiarid Mediterranean Regions. A Review, Part I: Increasing Energy Efficiency
Authors: Nikolaou Georgios , Neocleous Damianos , Christou Anastasis , Polycarpou Polycarpos , Kitta Evangelini , Katsoulas Nikolaos
Published: November 2021

Countries located in the Mediterranean region share many common features in terms of agricultural sustainability and economic realities of modern farming, as they are affected by water scarcity, energy use and climate suitability. Greenhouses are considered as a mitigation measure to combat climate change and as a sustainable production system. The majority of greenhouses in the Mediterranean region are rudimentary, while those in Central and North Europe are characterized by equipment of a high technological level for greenhouse climate and fertigation management. However, the technological innovations and research originating from Central and North Europe glasshouses may not be appropriate for use in Mediterranean plastic greenhouses when considering the trade-off between agronomic needs and potential energy savings. Identifying energy measures suitable for the local climate will improve energy efficiency and crop performance toward the goal of greenhouse sustainability. This review mainly focuses on renewable and energy-efficient control systems in Mediterranean greenhouses, where crops such as tomato and cucumber are widely grown.
Identification of Long Non-Coding RNAs Associated with Tomato Fruit Expansion and Ripening by Strand-Specific Paired-End RNA Sequencing
Authors: Wang Jinyu , Feng Yan , Ding Xiaotao , Huo Jingtian , Nie Wen-Feng
Published: November 2021

As emerging essential regulators in plant development, long non-coding RNAs (lncRNAs) have been extensively investigated in multiple horticultural crops, as well as in different tissues of plants. Tomato fruits are an indispensable part of people’s diet and are consumed as fruits and vegetables. Meanwhile, tomato is widely used as a model to study the ripening mechanism in fleshy fruit. Although increasing evidence shows that lncRNAs are involved in lots of biological processes in tomato plants, the comprehensive identification of lncRNAs in tomato fruit during its expansion and ripening and their functions are partially known. Here, we performed strand-specific paired-end RNA sequencing (ssRNA-seq) of tomato Heinz1706 fruits at five different developmental stages, as well as flowers and leaves. We identified 17,674 putative lncRNAs by referencing the recently released SL4.0 and annotation ITAG4.0 in tomato plants. Many lncRNAs show different expression patterns in fleshy fruit at different developmental stages compared with leaves or flowers. Our results indicate that lncRNAs play an important role in the regulation of tomato fruit expansion and ripening, providing informative lncRNA candidates for further studies in tomato fruits. In addition, we also summarize the recent advanced progress in lncRNAs mediated regulation on horticultural fruits. Hence, our study updates the understanding of lncRNAs in horticultural plants and provides resources for future studies relating to the expansion and ripening of tomato fruits.
Traditional Herbal Remedies Used for Managing Anxiety and Insomnia in Italy: An Ethnopharmacological Overview
Authors: Motti Riccardo , de Falco Bruna
Published: November 2021

Anxiety and insomnia are among the most common mental health disorders and are a major cause of disability around the world. Traditional herbal medicines are receiving significant attention in global health debates. Several Italian regions maintain rural traditions and are among the most extensively studied areas of Europe regarding medicinal plant uses. The present overview aims to highlight the use of wild and cultivated plants, specifically as sedatives and for insomnia treatment in Italy, and to collect, analyze, and summarize the available literature about their pharmacological activity as well as clinical and pre-clinical studies concerning the most cited plants. In total, 106 wild taxa are used in Italy for sedative purposes. The plant species belong to 76 genera and 32 families, of which the most cited are Asteraceae (24.2%) and Lamiaceae (21.1%). Leaves (29%) and flowers (27%) are the plant parts mostly used as infusion (70%) and decoction (25%). Out of 106 taxa documented, only the most cited are analyzed in this overview (A. arvensis L., C. nepeta L., C. monogyna Jacq., H. lupulus L., L. nobilis L., L. angustifolia Mill., M. sylvestris L., M. chamomilla L., M. officinalis L., O. basilicum L., P. rhoeas L., P. somniferum L., R. officinalis L., T. platyphyllus Scop., and V. officinalis L.). Among the fifteen species selected, only seven have been studied for their pharmacological activity as hypnotic-sedatives. Future pre-clinical and clinical studies are needed to better clarify the mechanism of action of bioactive compounds and confirm the potential of these alternative therapies.
Effectiveness of a Sexuality Workshop for Nurse Aides in Long-Term Care Facilities
Authors: Yang Man-Hua , Yang Shu-Ting , Wang Tze-Fang , Chang Li-Chun
Published: November 2021

Background: In long-term care facilities, there are frequent conflicts related to elderly residents’ sexual expression. Nurse aides usually handle such conflicts with negative or negligent attitudes; therefore, elderly sexuality is considered “problem behavior” and is stigmatized. Objectives: This study aimed to improve elderly residents’ quality of sexual life by enhancing nurse aides’ knowledge and attitudes toward elderly sexuality through sexuality workshops. Methods: A quasi-experimental study was conducted with 64 nurse aides and 58 residents, who were divided into two groups, i.e., an experimental group and a control group, according to the floor where the residents resided. The nurse aides in the experimental group participated in sexuality workshops and were compared with those in the control group with respect to their knowledge of and attitudes toward sexuality; the residents’ quality of sexual life was also compared between groups. Results: Compared with the control group, in the experimental group, the nurse aides’ knowledge of and attitudes toward elderly sexuality as well as the residents’ quality of sexual life significantly and continually improved after the sexuality workshops. Conclusion: The four-week sexuality workshop is effective and may be used as an example in developing occupational education programs regarding elderly sexuality in long-term care facilities.
Gamified Dual-Task Training for Individuals with Parkinson Disease: An Exploratory Study on Feasibility, Safety, and Efficacy
Authors: Chua Lee-Kuen , Chung Yu-Chen , Bellard David , Swan Laura , Gobreial Nicole , Romano Amanda , Glatt Ryan , Bonaguidi Michael A. , Lee Darrin J. , Jin Yi , Liu Charles Y. , Fisher Beth E.
Published: November 2021

Objectives: The feasibility and safety of the use of neurorehabilitation technology (SMARTfit® Trainer system) by physical therapists in implementing a gamified physical-cognitive dual-task training (DTT) paradigm for individuals with Parkinson disease (IWPD) was examined. Additionally, the efficacy of this gamified DTT was compared to physical single-task training (STT), both of which were optimized using physio-motivational factors, on changes in motor and cognitive outcomes, and self-assessed disability in activities of daily living. Methods: Using a cross-over study design, eight participants with mild-to-moderate idiopathic PD (including one with mild cognitive impairment) completed both training conditions (i.e., gamified DTT and STT). For each training condition, the participants attended 2–3 sessions per week over 8.8 weeks on average, with the total amount of training being equivalent to 24 1 h sessions. A washout period averaging 11.5 weeks was inserted between training conditions. STT consisted of task-oriented training involving the practice of functional tasks, whereas for gamified DTT, the same task-oriented training was implemented simultaneously with varied cognitive games using an interactive training system (SMARTfit®). Both training conditions were optimized through continual adaptation to ensure the use of challenging tasks and to provide autonomy support. Training hours, heart rate, and adverse events were measured to assess the feasibility and safety of the gamified DTT protocol. Motor and cognitive function as well as perceived disability were assessed before and after each training condition. Results: Gamified DTT was feasible and safe for this cohort. Across participants, significant improvements were achieved in more outcome measures after gamified DTT than they were after STT. Individually, participants with specific demographic and clinical characteristics responded differently to the two training conditions. Conclusion: Physical therapists’ utilization of technology with versatile hardware configurations and customizable software application selections was feasible and safe for implementing a tailor-made intervention and for adapting it in real-time to meet the individualized, evolving training needs of IWPD. Specifically in comparison to optimized STT, there was a preliminary signal of efficacy for gamified DTT in improving motor and cognitive function as well as perceived disability in IWPD.
Acute-Stage Mental Health Symptoms by Natural Disaster Type: Consultations of Disaster Psychiatric Assistance Teams (DPATs) in Japan
Authors: Takagi Yoshifumi , Takahashi Sho , Fukuo Yasuhisa , Arai Tetsuaki , Tachikawa Hirokazu
Published: November 2021

This study analyzed the support activities that the Disaster Psychiatric Assistance Team (DPAT) in Japan provided following four previous disasters (a volcanic eruption, a mudslide, a flood, and an earthquake) to identify links between the disaster type and the characteristics of acute stage mental disorders observed. Using Disaster Mental Health Information Support System database records of consultations with patients supported by the DPAT during the survey period from 2013 (when DPAT was launched) to 2016, we performed cross-tabulations and investigated significant differences using chi-squared tests. For expected values less than 5, Fisher’s exact test was performed. Frequently occurring acute-stage symptoms after a disaster include anxiety, sleep problems, mood and affect, and physical symptoms. The affected population characteristics, victim attributes, severity of damage sustained, and evacuation status were the chief factors that influenced acute-stage mental health symptoms. The psychiatric symptoms detected in our study together with the results of diagnoses are important for determining the types of early interventions needed during the acute stage of a disaster. By sharing baseline mental health information, together with disaster-related characteristics highlighted in this study, mental health providers are better able to predict future possible mental disorders and symptoms.
Effect of Residents-as-Teachers in Rural Community-Based Medical Education on the Learning of Medical Students and Residents: A Thematic Analysis
Authors: Nishikura Nozomi , Ohta Ryuichi , Sano Chiaki
Published: November 2021

Residents-as-teachers (RaT) is a theoretical framework emphasizing the significance of the similar learning background of teachers and learners. In Japan, community-based medical education (CBME) is a practical approach to teaching family medicine. This study aimed to investigate the impact and challenges of RaT on the learning of medical students and residents in CBME at a rural community hospital in Japan. Over the course of a year, the researchers conducted one-on-one interviews with three residents and ten medical students participating in family medicine training at the hospital. The interviews were recorded and transcribed verbatim. Grounded theory was used in the data analysis to clarify the findings. Three key themes emerged from the research: lack of educational experience with RaT, effectiveness of RaT, and challenges of RaT. Although participants were prejudiced against RaT, they felt its implementation could facilitate the establishment of beneficial relationships between learners and teachers. They were also able to participate in medical teams effectively. The findings suggest that the increased participation of senior doctors in RaT could strengthen its learning effects. RaT in rural CBME should be applied in various contexts, and its effectiveness should be further investigated both qualitatively and quantitatively.
Transferring Knowledge on Motor Development to Socially Vulnerable Parents of Infants: The Practice of Health Visitors
Authors: Pedersen Marlene Rosager Lund , Stougaard Marianne Staal , Ibsen Bjarne
Published: November 2021

Parents are a determinant factor in a child’s development of motor skills. Studies show that programmes in which health visitors supervise parents may improve infants’ motor skills. This study examines which factors health visitors have found to enhance and hamper the implementation of a motor development programme among socially vulnerable parents of infants. The data consist of three group interviews with 4 health visitors in each (12 health visitors in total) and a subsequent member check with 27 health visitors. All were audio-recorded and transcribed verbatim, and a thematic analysis was conducted. The results show that according to the health visitors, the programme increases the ability and willingness of parents to engage in co-producing its implementation. In particular, the materials that they hand out to the parents enhance the implementation. On the other hand, they perceive the limited time provided for the implementation, together with the many pressing needs of the families, as hampering the implementation. Consequently, the study can inform future policies and programmes for frontline workers and socially vulnerable parents of infants.
Associations between Parent–Child Nature Visits and Sleep, Physical Activity and Weight Status among Finnish 3–6-Year-Olds
Authors: Kokkonen Juuli-Mari , Vepsäläinen Henna , Abdollahi Anna , Paasio Hanna , Ranta Samuli , Erkkola Maijaliisa , Roos Eva , Ray Carola
Published: November 2021

Nature visits and nature exposure have been shown to be favorably associated with children’s health and development, but the research regarding their associations with children’s lifestyle habits is limited. The current study aimed to investigate the associations between the frequency of parent–child nature visits and sleep, moderate-to-vigorous physical activity (MVPA) and weight status among three- to six-year-old Finnish preschoolers. Parents and their children (n = 864) participated in a cross-sectional DAGIS (increased health and wellbeing in preschools) study, which was conducted between 2015 and 2016 in Finland. In total, 798 parents answered a questionnaire on the frequency of parent–child nature visits, which also included questions on sociodemographic factors and their children’s sleep habits. Parents also reported children’s bedtimes and wake-up times and children wore an accelerometer for seven days. Trained researchers measured children’s weight and height. Linear and logistic regression analyses were conducted. More frequent parent–child nature visits were associated with children’s longer sleep duration at night, higher amounts of MVPA outside preschool time and, among girls, good sleep consistency. The frequency of parent–child nature visits was not significantly associated with whether children were overweight or obese or not. Promoting parent–child nature visits could be a cost-effective way to increase young children’s MVPA and enhance night-time sleep.
Organoleptic assessment of canned dairy products: features of carrying it out and interpreting the results
Authors: Guseva T.B. , Soldatova S.Yu. , Karanyan O.M.
Published: November 2021

The article describes the features of carrying out and interpreting the results of the organoleptic assessment of canned dairy products “Whole condensed milk with sugar”. The analysis of the qualitative properties of the product is presented for all the main parameters. The relationship between the organoleptic characteristics of the product and compliance with the technology for the production of canned dairy products, the characteristics of raw materials, technological modes of heat treatment, and storage conditions are considered in detail. Since the organoleptic and physicochemical characteristics of food products change over time, the authors conclude that, in addition to the input evaluation, periodic control of canned dairy products during storage should be provided.
Microbiological safety of poultry products in a modified controlled atmosphere during storage
Authors: Tyumentseva V.S. , Gorodova A.S. , Abdullaeva A.M.
Published: November 2021

The paper presents the materials for determining the changes in microbiological parameters of poultry products packaged in a modified controlled atmosphere (MCA). It was found that MCA allows stopping the growth of unwanted microbiota, including pathogenic one, in poultry meat and poultry products due to correctly selected components for the controlled atmosphere.
Microphone array speech enhancement based on optimized IMCRA
Authors: Li Qiuying , Zhang Tao , Geng Yanzhang , Gao Zhen
Published: November 2021

Microphone array speech enhancement algorithm uses temporal and spatial informa- tion to improve the performance of speech noise reduction significantly. By combining noise estimation algorithm with microphone array speech enhancement, the accuracy of noise estimation is improved, and
the computation is reduced. In traditional noise es- timation algorithms, the noise power spectrum is not updated in the presence of speech, which leads to the delay and deviation of noise spectrum estimation. An optimized im- proved minimum controlled recursion average speech enhancement
algorithm, based on a microphone matrix is proposed in this paper. It consists of three parts. The first part is the preprocessing, divided into two branches: the upper branch enhances the speech signal, and the lower branch gets the noise. The second part is the optimized improved minimum
controlled recursive averaging. The noise power spectrum is updated not only in the non-speech segments but also in the speech segments. Fi- nally, according to the estimated noise power spectrum, the minimum mean-square error log-spectral amplitude algorithm is used to enhance speech. Testing
data are from TIMIT and Noisex-92 databases. Short-time objective intelligibility and seg- mental signal-to-noise ratio are chosen as evaluation metrics. Experimental results show that the proposed speech enhancement algorithm can improve the segmental signal-to-noise ratio and short-time
objective intelligibility for various noise types at different signal-to-noise ratio levels.