bluuebunny/crossref_metadata_2025 huggingface dataset:
AMERICAN INDIAN ENTREPRENEURS' PERCEPTIONS OF THEIR SUCCESS IN ESTABLISHING BUSINESSES ON OR NEAR RESERVATIONS IN WISCONSIN
Authors: ERDMANN STEPHANIE J.
Published: September 2016

The purpose of this phenomenological study is to explore a sample of American Indian entrepreneurs' perceptions of why and how they became successful owners of businesses on or near reservations in Wisconsin. The results provide insight about how to encourage and support potential American Indian entrepreneurs to open more businesses on or near reservation lands to create viable economies. This explorative study used planned inquiry to gather qualitative data by conducting in-depth interviews and collecting supportive data through critical incidents. The participants in the study include seven current American Indian business owners. Based on the findings, analysis and results of the research study, recommendations have been developed for tribal leaders, tribal educators, government agencies, and for further research. The conclusions to the research, which addressed the six themes and their findings, show that successful American Indian entrepreneurs (a) have been exposed to entrepreneurship and entrepreneurial ideals; (b) have an understanding of business concepts; (c) have been educated and have sought additional knowledge; (d) have participated in special programs with tribal preferences; (e) are risk-adverse to personal financial distress and (f) have nurtured relationships between and among themselves and family as well as between and among their communities.
Optimization Models for Three On-Chip Network Problems
Authors: Vaish Nilay , Ferris Michael C. , Wood David A.
Published: September 2016

We model three on-chip network design problems—memory controller placement, resource allocation in heterogeneous on-chip networks, and their combination—as mathematical optimization problems. We model the first two problems as mixed integer linear programs. We model the third problem as a mixed integer nonlinear program, which we then linearize exactly. Sophisticated optimization algorithms enable solutions to be obtained much more efficiently. Detailed simulations using synthetic traffic and benchmark applications validate that our designs provide better performance than solutions proposed previously. Our work provides further evidence toward suitability of optimization models in searching/pruning architectural design space.
GPUnet
Authors: Silberstein Mark , Kim Sangman , Huh Seonggu , Zhang Xinya , Hu Yige , Wated Amir , Witchel Emmett
Published: September 2016

Despite the popularity of GPUs in high-performance and scientific computing, and despite increasingly general-purpose hardware capabilities, the use of GPUs in network servers or distributed systems poses significant challenges.

GPUnet is a native GPU networking layer that provides a socket abstraction and high-level networking APIs for GPU programs. We use GPUnet to streamline the development of high-performance, distributed applications like in-GPU-memory MapReduce and a new class of low-latency, high-throughput GPU-native network services such as a face verification server.
A Survey of Wearable Biometric Recognition Systems
Authors: Blasco Jorge , Chen Thomas M. , Tapiador Juan , Peris-Lopez Pedro
Published: September 2016

The growing popularity of wearable devices is leading to new ways to interact with the environment, with other smart devices, and with other people. Wearables equipped with an array of sensors are able to capture the owner’s physiological and behavioural traits, thus are well suited for biometric authentication to control other devices or access digital services. However, wearable biometrics have substantial differences from traditional biometrics for computer systems, such as fingerprints, eye features, or voice. In this article, we discuss these differences and analyse how researchers are approaching the wearable biometrics field. We review and provide a categorization of wearable sensors useful for capturing biometric signals. We analyse the computational cost of the different signal processing techniques, an important practical factor in constrained devices such as wearables. Finally, we review and classify the most recent proposals in the field of wearable biometrics in terms of the structure of the biometric system proposed, their experimental setup, and their results. We also present a critique of experimental issues such as evaluation and feasibility aspects, and offer some final thoughts on research directions that need attention in future work.
Protostellar Outflows
Authors: Bally John
Published: September 2016

Outflows from accreting, rotating, and magnetized systems are ubiquitous. Protostellar outflows can be observed from radio to X-ray wavelengths in the continuum and a multitude of spectral lines that probe a wide range of physical conditions, chemical phases, radial velocities, and proper motions. Wide-field visual and near-IR data, mid-IR observations from space, and aperture synthesis with centimeter- and millimeterwave interferometers are revolutionizing outflow studies. Many outflows originate in multiple systems and clusters. Although most flows are bipolar and some contain highly collimated jets, others are wide-angle winds, and a few are nearly isotropic and exhibit explosive behavior. Morphologies and velocity fields indicate variations in ejection velocity, mass-loss rate, and in some cases, flow orientation and degree of collimation. These trends indicate that stellar accretion is episodic and often occurs in a complex dynamical environment. Outflow power increases with source luminosity but decreases with evolutionary stage. The youngest outflows are small and best traced by molecules such as CO, SiO, H2O, and H2. Older outflows can grow to parsec scales and are best traced by shock-excited atoms and ions such as hydrogen-recombination lines, [Sii], and [Oii]. Outflows inject momentum and energy into their surroundings and provide an important mechanism in the self-regulation of star formation. However, momentum injection rates remain uncertain with estimates providing lower bounds.
Accretion onto Pre-Main-Sequence Stars
Authors: Hartmann Lee , Herczeg Gregory , Calvet Nuria
Published: September 2016

Accretion through circumstellar disks plays an important role in star formation and in establishing the properties of the regions in which planets form and migrate. The mechanisms by which protostellar and protoplanetary disks accrete onto low-mass stars are not clear; angular momentum transport by magnetic fields is thought to be involved, but the low-ionization conditions in major regions of protoplanetary disks lead to a variety of complex nonideal magnetohydrodynamic effects whose implications are not fully understood. Accretion in pre-main-sequence stars of masses ≲1M⊙ (and in at least some 2–3-M⊙ systems) is generally funneled by the stellar magnetic field, which disrupts the disk at scales typically of order a few stellar radii. Matter moving at near free-fall velocities shocks at the stellar surface; the resulting accretion luminosities from the dissipation of kinetic energy indicate that mass addition during the T Tauri phase over the typical disk lifetime ∼3 Myr is modest in terms of stellar evolution, but is comparable to total disk reservoirs as estimated from millimeter-wave dust emission (∼10−2 M⊙). Pre-main-sequence accretion is not steady, encompassing timescales ranging from approximately hours to a century, with longer-timescale variations tending to be the largest. Accretion during the protostellar phase—while the protostellar envelope is still falling onto the disk—is much less well understood, mostly because the properties of the central obscured protostar are difficult to estimate. Kinematic measurements of protostellar masses with new interfometric facilities should improve estimates of accretion rates during the earliest phases of star formation.
Best-Quality Vessel Identification Using Vessel Quality Measure in Multiple-Phase Coronary CT Angiography
Authors: Hadjiiski Lubomir , Liu Jordan , Chan Heang-Ping , Zhou Chuan , Wei Jun , Chughtai Aamer , Kuriakose Jean , Agarwal Prachi , Kazerooni Ella
Published: September 2016

The detection of stenotic plaques strongly depends on the quality of the coronary arterial tree imaged with coronary CT angiography (cCTA). However, it is time consuming for the radiologist to select the best-quality vessels from the multiple-phase cCTA for interpretation in clinical practice. We are developing an automated method for selection of the best-quality vessels from coronary arterial trees in multiple-phase cCTA to facilitate radiologist’s reading or computerized analysis. Our automated method consists of vessel segmentation, vessel registration, corresponding vessel branch matching, vessel quality measure (VQM) estimation, and automatic selection of best branches based on VQM. For every branch, the VQM was calculated as the average radial gradient. An observer preference study was conducted to visually compare the quality of the selected vessels. 167 corresponding branch pairs were evaluated by two radiologists. The agreement between the first radiologist and the automated selection was 76% with kappa of 0.49. The agreement between the second radiologist and the automated selection was also 76% with kappa of 0.45. The agreement between the two radiologists was 81% with kappa of 0.57. The observer preference study demonstrated the feasibility of the proposed automated method for the selection of the best-quality vessels from multiple cCTA phases.
An Experimental Review on Elliptic Flow of Strange and Multistrange Hadrons in Relativistic Heavy Ion Collisions
Authors: Shi Shusu
Published: September 2016

Strange hadrons, especially multistrange hadrons, are good probes for the early partonic stage of heavy ion collisions due to their small hadronic cross sections. In this paper, I give a brief review on the elliptic flow measurements of strange and multistrange hadrons in relativistic heavy ion collisions at Relativistic Heavy Ion Collider (RHIC) and Large Hadron Collider (LHC).
Spectrum Assignment Algorithm for Cognitive Machine-to-Machine Networks
Authors: Rostami Soheil , Alabadi Sajad , Noori Soheir , Shihab Hayder Ahmed , Arshad Kamran , Rapajic Predrag
Published: September 2016

A novel aggregation-based spectrum assignment algorithm for Cognitive Machine-To-Machine (CM2M) networks is proposed. The introduced algorithm takes practical constraints including interference to the Licensed Users (LUs), co-channel interference (CCI) among CM2M devices, and Maximum Aggregation Span (MAS) into consideration. Simulation results show clearly that the proposed algorithm outperforms State-Of-The-Art (SOTA) algorithms in terms of spectrum utilisation and network capacity. Furthermore, the convergence analysis of the proposed algorithm verifies its high convergence rate.
A Combination of Intrastromal and Intracameral Injections of Amphotericin B in the Treatment of Severe Fungal Keratitis
Authors: Hu Jianzhang , Zhang Jingjin , Li Yanling , Han Xiaoli , Zheng Weidong , Yang Juan , Xu Guoxing
Published: September 2016

Purpose. To evaluate the efficacy of a combination of intrastromal and intracameral injections of amphotericin B in the treatment of severe recalcitrant fungal keratitis.Methods. Patients with severe fungal keratitis who were resistant to conventional antifungal medical treatments and needed potential surgical intervention were recruited at the First Affiliated Hospital of Fujian Medical University between January 2012 and July 2013. The patients were treated with a combination of intrastromal and intracameral injections of amphotericin B (25 μg/mL and 50 μg/mL, resp.). Selectively repeated injections were performed as necessary. The efficacy, complications, and outcome were evaluated.Results. Nine patients (9 eyes) were involved in this study. All 9 cases responded favorably, and the clinical appearance of serious corneal damage and intraocular extension was resolved after the treatment. Four eyes required only 1 injection, and 5 eyes required repeated injections. Seven corneal ulcers healed with leucoma, and 2 healed with adherent leucoma. All of our cases had a marked increase in the anterior chamber reaction and pain immediately after the injection. There was no obvious clinical evidence of corneal or lenticular toxicity in any patient.Conclusions. A combination of intrastromal and intracameral injections of amphotericin B may be safe and effective for the treatment of severe fungal keratitis that is resistant to conventional therapy.
Functional and Structural Consequences of NineCYP21A2Mutations Ranging from Very Mild to Severe Effects
Authors: de Paula Michelatto Débora , Karlsson Leif , Lusa Ana Letícia Gori , Silva Camila D’Almeida Mgnani , Östberg Linus Joakim , Persson Bengt , Guerra-Júnior Gil , Lemos-Marini Sofia Helena Valente de , Barbaro Michela , de Mello Maricilda Palandi , Lajic Svetlana
Published: September 2016

We present the functional and structural effects of seven novel (p.Leu12Met, p.Arg16Cys, p.Ser101Asn, p.Ser202Gly, p.Pro267Leu, p.Gln389_Ala391del, and p.Thr450Met) and two previously reported but not studied (p.Ser113Phe and p.Thr450Pro)CYP21A2mutations. Functional analyses were complemented within silicoprediction of mutation pathogenicity based on the recently crystallized human CYP21A2 structure. Mutated proteins were transiently expressed in COS-1 cells and enzyme activities towards 17-hydroxyprogesterone and progesterone were determined. Residual enzyme activities between 43% and 97% were obtained for p.Arg16Cys, p.Ser101Asn, p.Ser202Gly, p.Pro267Leu, and p.Thr450Met, similar to the activities of the well-known nonclassic mutations p.Pro453Ser and p.Pro482Ser, whereas the p.Leu12Met variant showed an activity of 100%. Conversely, the novel p.Ser113Phe, p.Gln389_Ala391del, and p.Thr450Pro mutations drastically reduced the enzyme function below 4%. TheKmvalues for all novel variants were in the same order of magnitude as for the wild-type protein except for p.The450Met. The maximum velocity was decreased for all mutants except for p.Leu12Met. We conclude that p.Leu12Met is a normal variant; the mutations p.Arg16Cys, p.Ser101Asn, p.Ser202Gly, p.Pro267Leu, and p.Thr450Met could be associated with very mild nonclassic CAH, and the mutations p.Ser113Phe, p.Gln389_Ala391del, and p.Thr450Pro are associated with classic CAH. The obtained residual activities indicated a good genotype-phenotype correlation.
Mesenchymal Stem Cell‐Based Therapy for Kidney Disease: A Review of Clinical Evidence
Authors: Peired Anna Julie , Sisti Alessandro , Romagnani Paola
Published: September 2016

Mesenchymal stem cells form a population of self‐renewing, multipotent cells that can be isolated from several tissues. Multiple preclinical studies have demonstrated that the administration of exogenous MSC could prevent renal injury and could promote renal recovery through a series of complex mechanisms, in particular via immunomodulation of the immune system and release of paracrine factors and microvesicles. Due to their therapeutic potentials, MSC are being evaluated as a possible player in treatment of human kidney disease, and an increasing number of clinical trials to assess the safety, feasibility, and efficacy of MSC‐based therapy in various kidney diseases have been proposed. In the present review, we will summarize the current knowledge on MSC infusion to treat acute kidney injury, chronic kidney disease, diabetic nephropathy, focal segmental glomerulosclerosis, systemic lupus erythematosus, and kidney transplantation. The data obtained from these clinical trials will provide further insight into safety, feasibility, and efficacy of MSC‐based therapy in renal pathologies and allow the design of consensus protocol for clinical purpose.
A Green Approach for the Synthesis of Silver Nanoparticles Using Ultrasonic Radiation’s Times in Sodium Alginate Media: Characterization and Antibacterial Evaluation
Authors: Faried Miftah , Shameli Kamyar , Miyake Mikio , Hajalilou Abdollah , Zamanian Ali , Zakaria Zuriati , Abouzari-lotf Ebrahim , Hara Hirofumi , Ahmad Khairudin Nurul Bahiyah Bt , Binti Mad Nordin Mariam Firdhaus
Published: September 2016

The synthesis of silver nanoparticles (Ag-NPs) was achieved by a simple green chemistry procedure using sodium alginate (Na-Alg) under ultrasonic radiation as a stabilizer and physical reducing agent. The effect of radiation time on the synthesis of Ag-NPs was carried out at room temperature until 720 min. The successful formation of Ag-NPs has been confirmed by UV-Vis, XRD, TEM, FESEM-EDX, zeta potential, and FT-IR analyses. The surface plasmon resonance band appeared at the range of 452–465 nm that is an evidence of formation of Ag-NPs. The XRD study showed that the particles are crystalline structure in nature, with a face-centered cubic (fcc) structure. The TEM study showed the Ag-NPs have average diameters of around 20.16–22.38 nm with spherical shape. The FESEM-EDX analysis confirmed the spherical shape of Ag-NPs on the surface of Alg and the element of Ag with the high purity. The zeta potential showed high stability of Alg/Ag-NPs especially after 720 min irradiation with value of −67.56 mV. The FT-IR spectrum confirmed that the Ag-NPs have been capped by the Alg with van der Waals interaction. The Alg/Ag-NPs showed the antibacterial activity against Gram-positive and Gram-negative bacteria. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field.
Modelling the Impact of Government Policies on Import on Domestic Price of Indian Gold Using ARIMA Intervention Method
Authors: Unnikrishnan Jyothi , Suresh Kodakanallur Krishnaswamy
Published: September 2016

The study attempts to determine the impact of government policies of import of gold in India on the domestic price of gold during 2013 using Autoregressive Integrated Moving Average (ARIMA) intervention model. 2013 was an amazing year for Indian gold market where the price had reached its zenith. In April 2013, to curb a record trade deficit, India imposed an import duty of 10 percent on gold and tied imports for domestic consumption to exports, creating scarce supply of the yellow metal and boosting premiums to curtail the Current Account Deficit (CAD). The objective of the paper is to model the impact of this intervention by the government on the domestic price of Indian gold. Suitable ARIMA model is fit on the preintervention period and thereafter the effects of the interventions are analysed. The results indicate that ARIMA(1,1,1)is the most suitable model during preintervention period. Intervention analysis reveals that there is significant decrease in domestic price of gold by 56% from 2013. The model may be used by policymakers to analyse the future of gold before framing regulations and policies.
Molecular Targets and Emerging Therapeutic Options for Uterine Leiomyosarcoma
Authors: Miller Heather , Ike Chiemeka , Parma Jennifer , Masand Ramya P. , Mach Claire M. , Anderson Matthew L.
Published: September 2016

Uterine leiomyosarcoma (uLMS) is an aggressive malignancy characterized by its early metastasis, high rates of recurrence, and poor prognosis. Multiple obstacles complicate the clinical management of uLMS. These include the fact that most uLMS are typically identified only after a woman has undergone hysterectomy or myomectomy, the limited efficacy of adjuvant therapy for early stage disease, and the poor response of metastatic disease to current treatments. Here, we discuss recent insights into the molecular basis of uLMS and discuss emerging options for its clinical management. Particular attention is given to the biologic basis of these strategies with the goal of understanding the rationale motivating their use.
Abnormal Admission Chest X-Ray and MEWS as ICU Outcome Predictors in a Sub-Saharan Tertiary Hospital: A Prospective Observational Study
Authors: Ssemmanda Hannington , Luggya Tonny Stone , Lubulwa Clare , Muyinda Zeridah , Kwitonda Pascal , Wanzira Humphrey , Ejoku Joseph
Published: September 2016

Background. Critical care in Uganda is a neglected speciality and deemed costly with limited funding/prioritization. We studied admission X-ray and MEWS as mortality predictors of ICU patients requiring mechanical ventilation.Materials and Methods. We did a cross-sectional study in Mulago Hospital ICU and 87 patients for mechanical ventilation were recruited with mortality as the outcome of interest. Chest X-ray results were the main independent variable and MEWS was also gotten for all patients.Results. We recruited 87 patients; most were males (60.92%), aged between 16 and 45 years (59.77%), and most admissions for mechanical ventilation were from the Trauma Unit (30.77%). Forty-one (47.13%) of the 87 patients died and of these 34 (53.13%) had an abnormal CXR with an insignificant IRR = 1.75 (0.90–3.38) (p=0.062). Patients with MEWS ≥ 5 (pvalues = 0.018) and/or having an abnormal superior mediastinum (pvalues = 0.013) showed a positive association with mortality while having a MEWS≥5 had an incidence risk ratio = 3.29 (1.00–12.02) (p=0.018). MEWS was a good predictor of mortality (predictive value = 0.6739).Conclusion. Trauma (31%) caused most ICU admissions, having an abnormal admission chest X-rays positively associated with mortality and a high MEWS was also a good predictor of mortality.
Discontinuity Preserving Image Registration through Motion Segmentation: A Primal-Dual Approach
Authors: Kiriyanthan Silja , Fundana Ketut , Majeed Tahir , Cattin Philippe C.
Published: September 2016

Image registration is a powerful tool in medical image analysis and facilitates the clinical routine in several aspects. There are many well established elastic registration methods, but none of them can so far preserve discontinuities in the displacement field. These discontinuities appear in particular at organ boundaries during the breathing induced organ motion. In this paper, we exploit the fact that motion segmentation could play a guiding role during discontinuity preserving registration. The motion segmentation is embedded in a continuous cut framework guaranteeing convexity for motion segmentation. Furthermore we show that a primal-dual method can be used to estimate a solution to this challenging variational problem. Experimental results are presented for MR images with apparent breathing induced sliding motion of the liver along the abdominal wall.
Tidal Volume Delivery and Endotracheal Tube Leak during Cardiopulmonary Resuscitation in Intubated Newborn Piglets with Hypoxic Cardiac Arrest Exposed to Different Modes of Ventilatory Support
Authors: Mendler Marc R. , Weber Claudia , Hassan Mohammad A. , Huang Li , Mayer Benjamin , Hummler Helmut D.
Published: September 2016

Background: There are few data available on the interaction of inflations, chest compressions (CC), and delivery of tidal volumes in newborn infants undergoing resuscitation in the presence of endotracheal tube (ET) leaks. Objectives: To determine the effects of different respiratory support strategies along with CC on changes in tidal volume and ET leaks in hypoxic newborn piglets with cardiac arrest. Methods: Asphyxiated newborn piglets, intubated with weight-adapted uncuffed ET, were randomized into three groups and resuscitated according to ILCOR 2010 guidelines: (1) T-piece resuscitator (TPR) group = peak inspiratory pressure (PIP)/positive end-expiratory pressure (PEEP) 25/5 cm H2O, rate 30/min, inflations interposed between CC (3:1 ratio); (2) self- inflating bag (SIB) group = PIP 25 cm H2O without PEEP, rate 30/min, inflations interposed between CC (3:1 ratio), and (3) ventilator group = PIP/PEEP of 25/5 cm H2O, rate 30/min. CC were applied with a rate of 120/min without synchrony to inflations. Results: We observed a significant increase of leak (average increase 11.4%) when CC was added to respiratory support (p = 0.0001). Expired tidal volume was larger in the SIB group than in the two other modes which both applied PEEP. However, tidal volumes caused by CC only were larger in the two groups with PEEP than in the SIB group (without PEEP). Conclusions: There is interaction between lung inflations and CC affecting leak and delivery of tidal volume, which may be influenced by the mode/device used for respiratory support. Leak is larger in the presence of PEEP. However, CC cause additional tidal volume which is larger in the presence of PEEP.
Post Stroke Dysglycemia and Acute Infarct Volume Growth: A Study Using Continuous Glucose Monitoring
Authors: Shimoyama Takashi , Kimura Kazumi , Uemura Junichi , Saji Naoki , Shibazaki Kensaku
Published: September 2016

Background: The aim of the present study was to clarify the effect of glucose profiles after stroke, which was assessed by a continuous glucose monitoring (CGM) device. Methods: Acute ischemic stroke patients within 24 h of onset were prospectively studied. CGM was performed for 72 h after admission. CGM parameters were evaluated as follows: (1) mean glucose level, (2) area under the curve (AUC) for glucose level >140 mg/dl and (3) SD of the glucose level. Infarct volume was measured at admission and 24 and 72 h after admission using diffusion-weighted imaging. CGM data and infarct volume growth were compared at 24 and 72 h. Results: Seventy-eight patients were enrolled in the present study. Spearman's rank correlation coefficients showed that both the mean glucose level (r = 0.433, p < 0.001 for 24 h; r = 0.308, p = 0.006 for 72 h) and AUC >140 mg/dl (r = 0.417, p < 0.001 for 24 h; r = 0.277, p = 0.014 for 72 h) were significantly correlated with acute infarct volume growth. The SD of the glucose level was associated with infarct volume growth at 24 h (r = 0.303, p = 0.007), but not 72 h (r = 0.195, p = 0.088). Conclusion: Post-stroke hyperglycemia was associated with infarct volume growth during the acute phase of ischemic stroke.
Isolated Cystic Periventricular Leukomalacia Differs from Cystic Periventricular Leukomalacia with Intraventricular Hemorrhage in Prevalence, Risk Factors and Outcomes in Preterm Infants
Authors: Wang Lan-Wan , Lin Yung-Chieh , Tu Yi-Fang , Wang Shan-Tair , Huang Chao-Ching ,   for the Taiwan Premature Infant Developmental Collaborative Study Group
Published: September 2016

Background: Cystic periventricular leukomalacia (cPVL) is the most severe white matter injury and is often associated with intraventricular hemorrhage (IVH) in preterm infants. Objective: The aim of this study was to investigate the prevalence, risk factors and neurodevelopmental outcomes of isolated cPVL and cPVL with low-grade and high-grade IVH in premature infants. Methods: From 2001 to 2012, 9,964 infants with <31 weeks' gestational age (GA) admitted to Taiwan hospitals were enrolled. cPVL was classified into three groups: isolated cPVL, cPVL with low-grade (I/II) IVH, and cPVL with high-grade (III) IVH. Results: Of 7,805 infants with complete ultrasound data, 286 (3.7%) had cPVL. Among the cPVL infants, 93 (32.5%) were isolated, 118 (41.3%) had low-grade IVH and 75 (26.2%) had high-grade IVH. The risk of cPVL with IVH was significantly higher among infants with <27 weeks' GA than those with ≥27 weeks' GA, in contrast to that of isolated cPVL. Using infants without cPVL and IVH as the reference group, the most significant predictor of isolated cPVL was neonatal sepsis (odds ratio 2.39; 95% confidence interval 1.52-3.77), while 5-min Apgar score <5 (2.50; 1.48-4.21) and prolonged mechanical ventilation (2.19; 1.42-3.42) were associated with cPVL with low-grade IVH, and GA <27 weeks (2.63; 1.56-4.42), pneumothorax (3.04; 1.40-6.65) and prolonged mechanical ventilation (3.36; 1.88-6.01) contributed to cPVL with high-grade IVH. cPVL infants with low-grade and high-grade IVH had a higher risk of abnormal neurodevelopmental outcomes than infants with isolated cPVL at the age of 24 months. Conclusions: Isolated cPVL, cPVL with low-grade IVH and cPVL with high-grade IVH had different risk factors and neurodevelopmental outcomes, suggestive of different causal pathways.
Antiangiogenic Therapy Induces Hepatic Tumor Vascular Network Rearrangement to Receive Perfusion via the Portal Vein and Hepatic Artery
Authors: Kang Wonseok , Lim Joon Seok , Park Mi-Suk , Koh Gou Young , Kim Honsoul
Published: September 2016

Purpose: Hepatic malignancies can easily develop resistance to antiangiogenic therapy, but the underlying mechanism remains poorly understood. This study explores whether antiangiogenic therapy influences the tumor vascular network and/or the vessels feeding the hepatic tumor. Methods: Mice implanted with Lewis lung carcinoma (LLC) cells were subcutaneously injected 3 times (once every other day starting 1 week after LLC implantation) with either an antiangiogenic agent [vascular endothelial growth factor (VEGF)-Trap] or control agent (bovine serum albumin) at a dose of 25 mg/kg before performing angiography. Hepatic arteriography and portography were performed using a vascular cast method with vascular latex. Results: Arteriography of the control-treated LLC-implanted mice showed marked staining of the mass with a prominent feeding artery, suggesting that the tumor is supplied by arterial perfusion. No significant staining was observed on portography. By contrast, 33% (n = 3/9) of the LLC-implanted mice treated with the antiangiogenic agent VEGF-Trap showed intratumoral staining during portography, indicating that these tumors received perfusion via the portal vein. Conclusion: Antiangiogenic treatment can induce rearrangement of the hepatic tumor vascular network to establish communication with the portal vein. This implies that hepatic tumors can develop resistance to antiangiogenic therapy by maintaining perfusion through portal venous perfusion.
Circulating Fibronectin and Plasminogen Activator Inhibitor-2 Levels as Possible Predictors of Recurrent Placental Syndrome: An Exploratory Study
Authors: Severens-Rijvers Carmen A.H. , Al-Nasiry Salwan , Ghossein-Doha Chahinda , Marzano Sara , Ten Cate Hugo , Winkens Bjorn , Spaanderman Marc A.E. , Peeters Louis L.H.
Published: September 2016

Background/Aim: Placental syndromes (PS) are characterized by endothelial dysfunction complicating placental dysfunction. Possible markers for endothelial dysfunction and amount of trophoblast are fibronectin and plasminogen activator inhibitor-2 (PAI-2), respectively. We aimed (1) to determine whether in women with recurrent PS (rPS), this complication is preceded by deviating fibronectin- and PAI-2-levels, and (2) whether this is dependent on pre-pregnant plasma volume (PV). Methods: In 36 former patients, we determined fibronectin- and PAI-2-levels in blood-samples collected preconceptionally and at 12-16 weeks in their next pregnancy. Differences were analyzed between pregnancies with rPS (n = 12) and without rPS (non-rPS, n = 24) using linear mixed models, with subanalyses based on pre-pregnant normal or subnormal PV. Results: We observed higher fibronectin-levels at 12-16 weeks (p < 0.05 and p < 0.01, respectively) and lower PAI-2-levels at 16 weeks (p < 0.01) in the rPS subgroup, the intergroup differences being larger in women with subnormal PV. Conclusion: We showed that former PS patients who developed rPS have raised fibronectin- and reduced PAI-2-levels already in early/mid pregnancy. These deviations are even more prominent in women with subnormal pre-pregnant PV, supporting development of a 2-step screening program for former patients to identify the high-risk subgroup of women who may benefit from closer surveillance.
Insulin Prevents Hyperfiltration and Proteinuria but Not Glomerular Hypertrophy and Increases Mesangial Matrix Expansion in Diabetic Rats
Authors: Malatiali Slava , Francis Issam , Barac-Nieto Mario
Published: September 2016

Objective: The aim of this work was to study the effect of 7 days of strict glycemic control with insulin on glomerular function and structure in streptozotocin (STZ)-diabetic rats. Materials and Methods: Three groups of adult male Fischer rats were studied: controls (n = 15), diabetics (n = 15), and insulin-treated diabetics (n = 15). Diabetes was induced by treating the rats with STZ (55 mg/kg i.p.). One week after the induction of diabetes, blood glucose, protein excretion rate (PER), glomerular filtration rate (GFR), and renal plasma flow (RPF) were estimated in each group. Furthermore, morphometric analysis was performed to estimate the tuft volume and changes in mesangial matrix area. The results are expressed as the mean ± SEM. Results: STZ diabetes caused significant increases in GFR (0.89 ± 0.1 to 1.21 ± 0.1 mL/min/100 g; p < 0.01) and RPF (1.78 ± 0.37 to 3.32 ± 0.6 mL/min/100 g; p < 0.05). Furthermore, the diabetic rats had higher glomerular volumes but mesangial matrix areas similar to controls. Insulin treatment prevented the increases in blood glucose (4.5 ± 0.2 mM), PER (66.1 ± 7.8 mg/day), GFR (0.6 ± 0.07 mL/min/100 g), and RPF (1.72 ± 0.36 mL/min/100 g), but did not prevent glomerular hypertrophy (21.7% increase), but induced mesangial matrix expansion (25% increase). Conclusions: Insulin prevented the diabetes-induced hyperfiltration and proteinuria, but did not prevent glomerular growth, and induced mesangial expansion. Hyperglycemic episodes could be partly responsible for persistent glomerular growth and accelerated mesangial growth.
Características seminais de ovinos suplementados ou não com uréia e diferentes fontes de enxofre
Authors: Hamilton Thais Rose dos Santos , Lucci Carlos De Sousa , Madureira Ed Hoffmann , Narimatsu Suelen , Goulart Rodrigo Hayashi , GIL Jefferson , Fontolan Valter , Baruselli Marcos Sampaio
Published: September 2016

Doze carneiros machos adultos mestiços Santa Inês de mesma idade e porte semelhante foram empregados em um delineamento inteiramente casualizado, por um período experimental de 60 dias. Os animais foram distribuídos para três tratamentos: A. 100% das exigências em proteína degradável no rúmen (controle); B. 100% das exigências em proteína degradável no rúmen + 3% de uréia + enxofre (99% S) e C. 100% das exigências em proteína degradável no rúmen + 3% de uréia + enxofre quelatado (21,5% S). Semanalmente foram colhidas amostras de sêmen obtidas com emprego de vagina artificial e de sangue para determinação da concentração de nitrogênio uréico plasmático, assim como realizadas pesagens dos animais e aferições de circunferência escrotal. No sêmen foram analisados: volume e turbilhonamento; vigor, motilidade e concentração espermática; total de espermatozóides e total de espermatozóides viáveis no ejaculado; integridade de membrana e de acrossoma; morfologia espermática e concentração de nitrogênio uréico no plasma seminal. Os animais suplementados com uréia apresentaram níveis de N-uréico no plasma sanguíneo e seminal significativamente maiores que os encontrados nos do tratamentos controle (p<0,05). Houve diferença significativa entre as fontes de enxofre utilizadas (p<0,05) quanto às características do sêmen estudadas, o tratamento C apresentando valores maiores para turbilhonamento (4,57), motilidade espermática (85,69%), vigor espermático (4,66) e total de espermatozóides por ejaculado (9,02 x 10(9)), além de uma porcentagem inferior de defeitos menores (5,37%) quando comparado ao tratamento B.
Efeitos de diferentes relações dietéticas de energia metabolizável: proteína bruta e do peso inicial de pintos sobre o desempenho e o rendimento de carcaça em frangos de corte fêmeas
Authors: Murarolli Rafael Azevedo , Alburqueque Ricardo De , Kobashigawa Estela , Murarolli Vinicius Diogo Azevedo , Trindade Neto Messias Alves de , Araújo Lúcio Francelino de
Published: September 2016

O estudo foi realizado para verificar o efeito de diferentes relações dietéticas de energia metabolizável:proteína bruta e do peso inicial de pintos de corte de 01 sobre o desempenho e rendimento de carcaça em frangos de corte. Foram utilizados 640 pintos, fêmeas, de uma linhagem comercial para corte (Cobb). O delineamento experimental utilizado foi de blocos ao acaso, em esquema fatorial 2 x 2, sendo empregados dois níveis de peso de pintos de 01 dia (38g e 46g) e dois níveis de relação EM:PB (Alto - 149,8; 174,4 e; 194,4 e, Baixo - 130,0; 150,0 e; 166,7, para ração inicial, crescimento e final, respectivamente) totalizando, assim, 4 tratamentos, cada um com 4 repetições de 40 aves, criados até 48 dias de idade. Foram avaliados o Consumo de Ração (CR), Ganho de Peso (GP), Conversão Alimentar (CA) durante o período experimental e, Rendimento de Carcaça e Rendimento de Cortes (Asa, coxa+sobrecoxa, peito, cabeça, pé e dorso) no final do período experimental. Nas variáveis CR e GP não foram observados efeitos significativos (P>;0,05) em nenhum dos tratamentos e períodos. Na variável CA, observou-se efeito significativo (P<0,05) no período de 1 a 35 dias de idade onde as aves alimentadas com a dieta com Relação EM:PB baixa apresentaram uma pior conversão alimentar, independente do peso inicial. No rendimento de carcaça houve efeito significativo (P<0,05), onde as aves com maior peso inicial obtiveram uma melhor porcentagem de rendimento quando receberam, durante todo o período de criação, dietas com relação EM:PB alta.
Pesquisa de anticorpos anti-Rickettsia rickettsii em eqüinos do Centro de Controle de Zoonoses do município de São Paulo (CCZ/SP)
Authors: Moraes-Filho Jonas , Horta Maurício Claudio , Pacheco Richard De Campos , Maeda Marly Matiko , Galano Arquimedes , Oliveira Maria Lucia de , Yai Lucia Eiko Oishi , Labruna Marcelo Bahia
Published: September 2016

A Febre Maculosa Brasileira (FMB) é uma zoonose transmitida por pelo menos duas espécies de carrapatos: Amblyomma cajennense e Amblyomma aureolatum. Os eqüinos assumem um importante papel de sentinela da FMB em áreas onde o carrapato vetor é o A. cajennense, por ser considerado hospedeiro primário dessa espécie de carrapato. O A. aureolatum, cujos hospedeiros primários são aves, alguns roedores silvestres e canídeos, é incriminado como vetor da doença na região da Grande São Paulo. Este trabalho objetivou pesquisar a presença de anticorpos contra Rickettsia rickettsii, agente da FMB, em eqüinos encaminhados ao Centro de Controle de Zoonoses do município de São Paulo (CCZ/SP) no período de 2003 a 2005. Após coleta de sangue, o soro obtido pela centrifugação foi submetido à reação de imunofluorescência indireta (RIFI) e os animais foram considerados positivos quando as amostras de soro apresentaram títulos >; 64. Durante os três anos, foram testados 363 amostras pela RIFI, sendo que 64 se mostraram reagentes: 6 (2003), 16 (2004) e 42 (2005). Os títulos finais variaram de 64 a 1.024. Os resultados obtidos demonstram uma baixa porcentagem de animais reagentes (17,6%) quando comparados com dados de literatura de áreas endêmicas para FMB onde o vetor é o A. cajennense, sugerindo não ser essa espécie responsável pela transmissão da doença na área de estudo
Infecção experimental de morcegos hematófagos Desmodus rotundus (E. Geoffroy) mantidos em cativeiro e alimentados com sangue desfibrinado adicionado de vírus da raiva
Authors: Souza Maria Conceição Aparecida Macedo , Nassar Alessandra Figueiredo de Castro , Cortez Adriana , Sakai Takeo , Itou Takuya , Cunha Elenice Maria Sequetin , Richtzenhain Leonardo José , Ito Fumio Honma
Published: September 2016

Em morcegos hematófagos, o hábito de compartilhar alimento poderia contribuir na transmissão oral do vírus da raiva. Para verificar esta hipótese, 10 morcegos Desmodus rotundus em cativeiro foram alimentados com sangue suíno desfibrinado, contendo suspensão de cérebros de camundongos infectados com vírus rábico PV. Outros 10 camundongos receberam sangue contendo suspensão cerebral de camundongos infectados com vírus de morcego hematófago (T-9/ 95). Um grupo de 10 camundongos foi inoculado intramuscularmente com suspensão de vírus T-9/95. Outros 20 morcegos foram mantidos sem tratamento e alimentados com sangue desfibrinado por 158 dias. Todos os animais encontrados mortos durante o período de observação ou sacrificados no final do experimento foram necropsiados e os cérebros e órgãos não-nervosos foram colhidos para a confirmação da raiva. Quatro morcegos inoculados intramuscularmente apresentaram raiva clínica, com sinais persistindo por 1-2 dias e os períodos de sobrevivência variaram de 11-14 dias. O diagnóstico da raiva inicialmente foi realizado somente com os fragmentos do cérebro, submetendo-os às provas de imunoflurescência direta (IFD) e inoculação em camundongos (IC). Subseqüentemente, os cérebros e os órgãos não-nervosos foram reexaminados com as técnicas de IFD, IC e heminested-polymerase chain reaction (ht-PCR). A ingestão do vírus PV causou raiva em dois morcegos, com período de sobrevivência de 25 e 32 dias, enquanto que os três morcegos que ingeriram o isolado T-9/95 apresentaram períodos de 26-31 dias. Embora encontrando resultados discrepantes entre as técnicas diagnósticas utilizadas, os vírus ingeridos pelos morcegos foram detectados no sistema nervoso central e outros órgãos não-nervosos, como nos morcegos inoculados intramuscularmente.
Arranjos configurados pelos nervos do plexo braquial no urubu (Coragyps atratus foetens -Linnaeus, 1758)
Authors: Moreira Pamela Rodrigues Reina , Souza Wilson Machado de , Souza Nair Trevisan Machado de , Carvalho Roberto Gameiro
Published: September 2016

O estudo anatômico da origem e distribuição do plexo braquial no urubu (Coragyps atratus foetens) foi efetuado em 14 animais, adultos,machos e fêmeas oriundos da região de Araçatuba (SP). Após a fixação em solução aquosa de formaldeído a 10,00%, realizou-se a dissecação bilateral da origem dos plexos braquiais e sua distribuição. A análise permitiu verificar, que o plexo braquial do urubu, possui quatro troncos, os quais originam-se dos ramos ventrais dos nervos espinhais cervicais onze (C11), doze (C12) e treze (C13) e dos ramos ventrais dos nervos espinhais torácicos um (T1) e (T2) em 100% dos casos em ambos antímeros. O primeiro tronco parte de C11 nos antímeros direito e esquerdo, e da margem cranial do cordão dorsal partem os nervos subescapular, subcoracoescapular e supracoracóide; em ambos antímeros (100%). O segundo tronco origina-se de C12 em ambos antímeros, dando origem ao cordão dorsal, dele partem os nervos axilar, radial e anconeal (100%). O terceiro tronco do plexo origina-se unicamente de C13 (100%) e o quarto tronco de T1 em ambos antímeros (100%). Os troncos de C12, C13 e T1 unem-se dando origem ao cordão ventral, o qual partem os nervos medianoulnar que se divide em mediano e ulnar e os nervos peitorais (100%). T2 emite filamentos que se unem à raiz nervosa de T1, sendo um filamento (35,55%) e dois filamentos (64,29%) no antímero direito, enquanto que no esquerdo um filamento (42,85%), dois filamentos (50%) e três filamentos (7,15%)
Morfologia das células do sangue periférico em emas (Rhea americana)
Authors: Fortes Eunice Anita de Moura , Sousa Antônio Francisco de , Almeida Ezequiel Cardozo Saraiva de , Conde Júnior Aírton Mendes , Moura Weber Leal de
Published: September 2016

A ema (Rhea americana) é uma ave sul-americana do grupo das ratitas, da ordem Rheiforme, freqüentemente explorada com fins econômicos, como pecuária alternativa em países europeus e sul-americanos. No Brasil, destaca-se o Rio Grande do Sul e, em fase inicial, o Nordeste. O presente estudo objetivou descrever a morfologia das células sangüíneas em ema. Neste trabalho foram utilizados dez exemplares, desconsiderando-se idade e sexo. Foram colhidos 3mL de sangue periférico por punção da veia braquial, com seringa descartável. As amostras foram utilizadas, em parte, na confecção de extensões coradas com Leishman. Feita a análise morfológica ao Microscópio de Luz, foram observados sete tipos celulares nucleados. O eritrócito mostrou-se elíptico, com núcleo geralmente condensado, de forma elíptica; citoplasma acidófilo. O trombócito apresentou-se elíptico, com núcleo localizado em um dos pólos; citoplasma pálido. Quanto aos leucócitos, de forma arredondada, entre os granulócitos os heterófilos apresentaram-se com núcleo excêntrico, condensado, lobulado; citoplasma rico em grânulos fusiformes de coloração salmão. Os eosinófilos distinguem-se dos heterófilos pelos grânulos arredondados eosinofílicos. Os basófilos destacam-se dos outros granulócitos pelo núcleo grande e central, com grânulos citoplasmáticos específicos arredondados e fortemente basofílicos. Entre os agranulócitos, os monócitos mostraram núcleo rineforme, freqüentemente central, de cromatina frouxa, com pequenas áreas de condensação; citoplasma levemente basofílico e com vacúolos. Os linfócitos apresentaram-se variados em forma e tamanho; núcleo grande com cromatina frouxa, com alguns nucléolos; citoplasma escasso e basofílico. As células do sangue periférico de Rhea americana apresentam ao Microscópio de Luz morfologia semelhante às demais aves já estudadas.
Detecção do DNA do herpesvírus eqüino 1 pela reação em cadeia pela polimerase em cavalos inoculados com a estirpe brasileira A4/72
Authors: Mori Enio , Mori Claudia Madalena Cabrera , Massironi Sílvia Maria Gomes , Cunha Elenice Maria Sequetin , Villalobos Eliana Monteforte Cassaro , Lara Maria do Carmo Custódio de Souza Hunold , Fernandes Wilson Roberto
Published: September 2016

Sete cavalos adultos de status sanitário convencional foram inoculados por via intranasal com a estirpe brasileira A4/72 do herpesvírus eqüino tipo 1 (EHV-1). Nos primeiros dez dias após a inoculação viral, todos os cavalos apresentaram manifestações de infecção respiratória leve e restrita às vias aéreas anteriores. Apesar de possuírem títulos de anticorpos neutralizantes antes da inoculação, alguns cavalos apresentaram soroconversão após o desafio viral. O EHV-1 não foi isolado a partir das secreções nasais e leucócitos sanguíneos periféricos (PBL) de nenhum animal. Entretanto, o DNA viral foi detectado pela reação em cadeia pela polimerase (PCR) nos PBL entre o terceiro e o oitavo dias pós-inoculação (d.p.i.) em todos os animais, indicando a ocorrência de viremia. Além disso, a prova de PCR detectou o vírus nas amostras do lavado broncoalveolar a partir do nono d.p.i. na maioria dos animais. Com base nos resultados obtidos, foi possível concluir que a PCR é uma técnica com alta sensibilidade e especificidade para o diagnóstico do EHV-1, capaz de detectar a presença do DNA viral mesmo quando não ocorre a constatação do agente pelos métodos tradicionais.
Perfil bioquímico de algumas enzimas no plasma sangüíneo de potras da raça Brasileiro de Hipismo (BH) criadas em Colina, Estado de São Paulo
Authors: Benesi Fernando José , Howard Denise Louise , Leal Marta Lizandra do Rego , Gacek Fernando , Souza José Adalmir Torres de , Fernandes Wilson Roberto
Published: September 2016

Com o propósito de avaliar o perfil bioquímico de algumas enzimas, em potras sadias da raça BH (Brasileiro de Hipismo), utilizaram-se 380 amostras de plasma sangüíneo colhidas de 19 animais desde o nascimento até 24 meses de vida. Na análise dos resultados evidenciou-se que os valores médios das enzimas FA (fosfatase alcalina) e CK (creatina quinase ) foram máximos entre o nascimento e 24 horas de vida (FA-1995.50 UI/ ; CK-189.13UI/L), enquanto que para a LD (lactato desidrogenase) e GGT (gama glutamiltransferase) as maiores magnitudes ocorreram, respectivamente, entre 3 e 4 dias (LD-479.11UI/L) e aos 10 dias de idade (GGT-18.70UI/L). As FA, CK, LD e GGT, mostraram diminuições dos valores médios, respectivamente, até 6 meses (FA-323.50UI/L), 20 dias (CK-51UI/ L), 19 meses (LD-214.00UI/L) e 4 meses (GGT-11.40UI/L) estabilizando-se a seguir, com pequenas variações. A atividade da AST (aspartato aminotransferase) que foi mínima logo após o nascimento (AST- 43.38UI/L), aumentou até os sete dias de vida (AST-110.89UI/L), e a seguir diminuiu progressivamente, com pequenas oscilações, até o final do estudo. Todas as enzimas avaliadas sofreram variações influenciadas pelo fator etário, particularmente no período inicial de vida dos animais estudados.