bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship between Dietary Patterns and Subjectively Measured Physical Activity in Japanese Individuals 85 Years and Older: A Cross-Sectional Study
Authors: Yu Tao , Oguma Yuko , Asakura Keiko , Takayama Michiyo , Abe Yukiko , Arai Yasumichi
Published: July 2022

High-quality diets and regular physical activity (PA) are considered healthy behaviors (HBs). HBs are associated with many health outcomes and are expected to improve quality of life. Although implementing HBs is important, the relationship between dietary patterns (DPs) and PA has not been well investigated, especially among those aged ≥ 85. This study used data from the Tokyo Oldest Old survey on Total Health study to examine the relationship between DPs and PA in a cross-sectional study. The dietary survey used the brief self-administered diet history questionnaire to estimate the intake of 58 foods. After energy adjustment, principal component analysis was performed to identify major DPs. A validated questionnaire was used to evaluate PA, and linear regression analysis was used to investigate the association between DPs and PA, considering confounders. A total of 519 participants were included. Three major DPs (‘Various plant foods’, ‘Fish and mushrooms’, ‘Cooked rice and miso soup’) were identified. ‘Various plant foods’ was similar to DPs previously named ‘Healthy’ or ‘Prudent’, and its trend was positively associated with higher PA. This study observed the implementation of HBs even among those aged ≥ 85, suggesting that a trend toward a healthier diet is associated with higher PA.
Assessment of SARS-CoV-2 Infection According to Previous Metabolic Status and Its Association with Mortality and Post-Acute COVID-19
Authors: de Arriba Fernández Alejandro , Alonso Bilbao José Luis , Espiñeira Francés Alberto , Cabeza Mora Antonio , Gutiérrez Pérez Ángela , Díaz Barreiros Miguel Ángel , Serra Majem Lluís
Published: July 2022

Background. SARS-CoV-2 infection was analyzed according to previous metabolic status and its association with mortality and post-acute COVID-19. Methods. A population-based observational retrospective study was conducted on a cohort of 110,726 patients aged 12 years or more who were diagnosed with COVID-19 infection between June 1st, 2021, and 28 February 2022 on the island of Gran Canaria, Spain. Results. In the 347 patients who died, the combination of advanced age, male sex, cancer, immunosuppressive therapy, coronary heart disease, elevated total cholesterol and reduced high-density lipoprotein cholesterol (HDL-C) was strongly predictive of mortality (p < 0.05). In the 555 patients who developed post-acute COVID-19, the persistence of symptoms was most frequent in women, older subjects and patients with obstructive sleep apnea syndrome, asthma, elevated fasting glucose levels or elevated total cholesterol (p < 0.05). A complete vaccination schedule was associated with lower mortality (incidence rate ratio (IRR) 0.5, 95%CI 0.39–0.64; p < 0.05) and post-acute COVID-19 (IRR 0.37, 95%CI 0.31–0.44; p < 0.05). Conclusions. Elevated HDL-C and elevated total cholesterol were significantly associated with COVID-19 mortality. Elevated fasting glucose levels and elevated total cholesterol were risk factors for the development of post-acute COVID-19.
Biomarker of Stress, Metabolic Syndrome and Human Health
Authors: Bouillon-Minois Jean-Baptiste , Dutheil Frédéric
Published: July 2022

Metabolic syndrome is a significant public health concern linked to the obesity pandemic [...]
The Use of 360-Degree Video in Developing Emotional Coping Skills (Reduced Anxiety and Increased Confidence) in Mental Health Nursing Students: A Protocol Paper
Authors: Laker Caroline , Knight-Davidson Pamela , Hawkes David , Driver Paul , Nightingale Maxine , Winter Ann , McVicar Andrew
Published: July 2022

Higher education institutions are uniquely placed to introduce emotional coping skills to promote resilience in pre-registration nurses in order to reduce anxiety and increase confidence before they enter clinical placement for the first time. In this qualitative study, we will explore the use of a 360-degree video in developing skills for coping. The participants will be mental health nursing students. We will develop a 360-degree video in collaboration with a mental health service user. All participants will watch the video. A sub-group will receive a supportive clinical supervision discussion within a cognitive reappraisal/solution-focused/VERA framework. We will record the experiences of the participant to explore: (1) how students felt about the use of 360-degree video, as an education tool to build skills of resilience; (2) whether the students involved felt more confident and less anxious about the situation in the video as a result of participating in the cognitive reappraisal/solution-focused/VERA supervision discussion.
Ginger Is a Potential Therapeutic for Chronic Toxoplasmosis
Authors: El-kady Asmaa M. , Al-Megrin Wafa Abdullah I. , Abdel-Rahman Iman A. M. , Sayed Eman , Alshehri Eman Abdullah , Wakid Majed H. , Baakdah Fadi M. , Mohamed Khalil , Elshazly Hayam , Alobaid Hussah M. , Qahl Safa H. , Elshabrawy Hatem A. , Younis Salwa S.
Published: July 2022

Background:Toxoplasma gondii (T. gondii) is an opportunistic parasite that causes serious diseases in humans, particularly immunocompromised individuals and pregnant women. To date, there are limited numbers of therapeutics for chronic toxoplasmosis which necessitate the discovery of effective and safe therapeutics. In the present study, we aimed to evaluate the antitoxoplasmosis potential of ginger extract in mice with experimentally induced chronic toxoplasmosis. Results: Treatment with ginger extract significantly reduced cysts count in the brains of T. gondii-infected mice with a marked alleviation of edema and inflammation, and a reversal of neuronal injury. Moreover, ginger extract treatment reduced inflammation in liver and lungs and protected hepatocytes from infection-induced degeneration. Consistently, apoptosis was significantly mitigated in the brains of ginger extract-treated mice compared to infected untreated animals or spiramycin-treated animals. Methods: Four groups of Swiss albino mice (10 mice each) were used. The first group was not infected, whereas 3 groups were infected with Me49 T. gondii strains. One infected group remained untreated (infected untreated), whereas the other two infected groups were treated with either ginger extract (250 mg/kg) or spiramycin (positive control; 100 mg/kg), respectively. The therapeutic potential of ginger extract was evaluated by calculation of the parasite burden in infected animals, and examination of the infected tissues for reduced pathologic changes. Conclusions: Our results showed for the first time that ginger extract exhibited marked therapeutic effects in mice with chronic T. gondii infection which indicates that it can be used as a safe and effective treatment for chronic toxoplasmosis.
Complete Genomes of Theileria orientalis Chitose and Buffeli Genotypes Reveal within Species Translocations and Differences in ABC Transporter Content
Authors: Yam Jerald , Bogema Daniel R. , Micallef Melinda L. , Djordjevic Steven P. , Jenkins Cheryl
Published: July 2022

Theileria orientalis causes losses to cattle producers in Eastern Asia, Oceania and, more recently, North America. One pathogenic genotype (Ikeda) has been sequenced to the chromosomal level, while only draft genomes exist for globally distributed Chitose and Buffeli genotypes. To provide an accurate comparative gene-level analysis and help further understand their pathogenicity, we sequenced isolates of the Chitose and Buffeli genotypes of T. orientalis using long-read sequencing technology. A combination of several long-read assembly methods and short reads produced chromosomal-level assemblies for both Fish Creek (Chitose) and Goon Nure (Buffeli) isolates, including the first complete and circular apicoplast genomes generated for T. orientalis. Comparison with the Shintoku (Ikeda) reference sequence showed both large and small translocations in T. orientalis Buffeli, between chromosomes 2 and 3 and chromosomes 1 and 4, respectively. Ortholog clustering showed expansion of ABC transporter genes in Chitose and Buffeli. However, differences in several genes of unknown function, including DUF529/FAINT-domain-containing proteins, were also identified and these genes were more prevalent in Ikeda and Chitose genotypes. Phylogenetics and similarity measures were consistent with previous short-read genomic analysis. The generation of chromosomal sequences for these highly prevalent T. orientalis genotypes will also support future studies of population genetics and mixed genotype infections.
Preparation and Storage of Cryoprecipitate Derived from Amotosalen and UVA-Treated Apheresis Plasma and Assessment of In Vitro Quality Parameters
Authors: Kovacic Krizanic Katarina , Prüller Florian , Rosskopf Konrad , Payrat Jean-Marc , Andresen Silke , Schlenke Peter
Published: July 2022

Cryoprecipitate is a plasma-derived blood product, enriched for fibrinogen, factor VIII, factor XIII, and von Willebrand factor. Due to infectious risk, the use of cryoprecipitate in Central Europe diminished over the last decades. However, after the introduction of various pathogen-reduction technologies for plasma, cryoprecipitate production in blood centers is a feasible alternative to pharmaceutical fibrinogen concentrate with a high safety profile. In our study, we evaluated the feasibility of the production of twenty-four cryoprecipitate units from pools of two units of apheresis plasma pathogen reduced using amotosalen and ultraviolet light A (UVA) (INTERCEPT® Blood System). The aim was to assess the compliance of the pathogen-reduced cryoprecipitate with the European Directorate for the Quality of Medicines (EDQM) guidelines and the stability of coagulation factors after frozen (≤−25 °C) storage and five-day liquid storage at ambient temperature post-thawing. All pathogen-reduced cryoprecipitate units fulfilled the European requirements for fibrinogen, factor VIII and von Willebrand factor content post-preparation. After five days of liquid storage, content of these factors exceeded the minimum values in the European requirements and the content of other factors was sufficient. Our method of production of cryoprecipitate using pathogen-reduced apheresis plasma in a jumbo bag is feasible and efficient.
Novel Pharmaceutical Strategies for Enhancing Skin Penetration of Biomacromolecules
Authors: Zhang Luyu , Dong Zirong , Liu Wenjuan , Wu Xiying , He Haisheng , Lu Yi , Wu Wei , Qi Jianping
Published: July 2022

Skin delivery of biomacromolecules holds great advantages in the systemic and local treatment of multiple diseases. However, the densely packed stratum corneum and the tight junctions between keratinocytes stand as formidable skin barriers against the penetration of most drug molecules. The large molecular weight, high hydrophilicity, and lability nature of biomacromolecules pose further challenges to their skin penetration. Recently, novel penetration enhancers, nano vesicles, and microneedles have emerged as efficient strategies to deliver biomacromolecules deep into the skin to exert their therapeutic action. This paper reviews the potential application and mechanisms of novel skin delivery strategies with emphasis on the pharmaceutical formulations.
Hybrid Nanosystems Based on Nicotinate-Functionalized Mesoporous Silica and Silver Chloride Nanoparticles Loaded with Phenytoin for Preventing Pseudomonas aeruginosa Biofilm Development
Authors: Ugalde-Arbizu Maider , Aguilera-Correa John Jairo , Mediero Aranzazu , Esteban Jaime , Páez Paulina L. , San Sebastian Eider , Gómez-Ruiz Santiago
Published: July 2022

Pseudomonas aeruginosa (PA) is one of the most common bacteria isolated from chronic wounds and burns. Its treatment is a challenge due to antimicrobial drug resistance and biofilm formation. In this context, this study aimed to perform the synthesis and full characterization of hybrid nanosystems based on mesoporous silica nanoparticles (MSNs) functionalized with a nicotinic ligand and silver chloride nanoparticles, both phenytoin sodium (Ph)-loaded and unloaded, to evaluate the antibacterial properties against three different strains of PA (including two clinical strains) in a planktonic state and as biofilms. Ph is a well-known proliferative agent, which was incorporated into the hybrid nanomaterials to obtain an effective material for tissue healing and prevention of infection caused by PA. The Ph-loaded materials promoted a quasi-complete inhibition of bacterial growth in wound-like medium and biofilm development, with values of 99% and 96%, respectively, with selectivity indices above the requirements for drugs to become promising agents for the topic preventive treatment of chronic wounds and burns.
Extracellular Alterations in pH and K+ Modify the Murine Brain Endothelial Cell Total and Phospho-Proteome
Authors: Wahl Jared R. , Vivek Anjali , Palomino Seph M. , Almuslim Moyad , Cottier Karissa E. , Langlais Paul R. , Streicher John M. , Vanderah Todd W. , Liktor-Busa Erika , Largent-Milnes Tally M.
Published: July 2022

Pathologies of the blood–brain barrier (BBB) have been linked to a multitude of central nervous system (CNS) disorders whose pathology is poorly understood. Cortical spreading depression (CSD) has long been postulated to be involved in the underlying mechanisms of these disease states, yet a complete understanding remains elusive. This study seeks to utilize an in vitro model of the blood–brain barrier (BBB) with brain endothelial cell (b.End3) murine endothelioma cells to investigate the role of CSD in BBB pathology by characterizing effects of the release of major pronociceptive substances into the extracellular space of the CNS. The application of trans-endothelial electrical resistance (TEER) screening, transcellular uptake, and immunoreactive methods were used in concert with global proteome and phospho-proteomic approaches to assess the effect of modeled CSD events on the modeled BBB in vitro. The findings demonstrate relocalization and functional alteration to proteins associated with the actin cytoskeleton and endothelial tight junctions. Additionally, unique pathologic mechanisms induced by individual substances released during CSD were found to have unique phosphorylation signatures in phospho-proteome analysis, identifying Zona Occludins 1 (ZO-1) as a possible pathologic “checkpoint” of the BBB. By utilizing these phosphorylation signatures, possible novel diagnostic methods may be developed for CSD and warrants further investigation.
Design Space Approach for the Optimization of Green Fluidized Bed Granulation Process in the Granulation of a Poorly Water-Soluble Fenofibrate Using Design of Experiment
Authors: Fayed Mohamed H. , Alalaiwe Ahmed , Almalki Ziyad S. , Helal Doaa A.
Published: July 2022

In the pharmaceutical industry, the systematic optimization of process variables using a quality-by-design (QbD) approach is highly precise, economic and ensures product quality. The current research presents the implementation of a design-of-experiment (DoE) driven QbD approach for the optimization of key process variables of the green fluidized bed granulation (GFBG) process. A 32 full-factorial design was performed to explore the effect of water amount (X1; 1–6% w/w) and spray rate (X2; 2–8 g/min) as key process variables on critical quality attributes (CQAs) of granules and tablets. Regression analysis have demonstrated that changing the levels of X1 and X2 significantly affect (p ≤ 0.05) the CQAs of granules and tablets. Particularly, X1 was found to have the pronounced effect on the CQAs. The GFBG process was optimized, and a design space (DS) was built using numerical optimization. It was found that X1 and X2 at high (5.69% w/w) and low (2 g/min) levels, respectively, demonstrated the optimum operating conditions. By optimizing X1 and X2, GFBG could enhance the disintegration and dissolution of tablets containing a poorly water-soluble drug. The prediction error values of dependent responses were less than 5% that confirm validity, robustness and accuracy of the generated DS in optimization of GFBG.
Another Move towards Bicalutamide Dissolution and Permeability Improvement with Acetylated β-Cyclodextrin Solid Dispersion
Authors: Volkova Tatyana V. , Simonova Olga R. , Perlovich German L.
Published: July 2022

The complex formation of antiandrogen bicalutamide (BCL) with methylated (Me-β-CD) and acetylated (Ac-β-CD) β-cyclodextrins was investigated in buffer solution pH 6.8. A two-fold strongly binding of BCL to Ac-β-CD as compared to Me-β-CD was revealed. The solid dispersion of BCL with Ac-β-CD was prepared by the mechanical grinding procedure to obtain the complex in the solid state. The BCL/Ac-β-CD complex was characterized by DSC, XPRD, FTIR, and SEM techniques. The effect of Ac-β-CD in the BCL solid dispersions on the non-sink dissolution/permeation simultaneous processes was disclosed using the side-by-side diffusion cell with the help of the cellulose membrane. The elevated dissolution of the ground complex, as compared to the raw drug as well as the simple physical mixture, accompanied by the supersaturation was revealed. Two biopolymers—polyvinylpyrrolidone (PVP, Mn = 58,000) and hydroxypropylmethylcellulose (HPMC, Mn ~ 10,000)—were examined as the precipitation inhibitors and were shown to be useful in prolonging the supersaturation state. The BCL/Ac-β-CD complex has the fastest dissolution rate in the presence of HPMC. The maximal concentration of the complex was achieved at a time of 20, 30, and 90 min in the pure buffer, with PVP and with HPMC, respectively. The effectiveness of the BCL dissolution (release) processes (illustrated by the AUCC(t) parameter) was estimated to be 7.8-, 5.8-, 3.0-, and 1.8-fold higher for BCL/Ac-β-CD (HPMC), BCL/Ac-β-CD (PVP), BCL/Ac-β-CD (buffer), and the BCL/Ac-β-CD physical mixture, respectively, as compared to the BCL_raw sample. The excipient gain factor (EGF), calculated for the dissolution of the BCL complex, was shown to be 2.6 in the presence of HPMC, which is 1.3-fold greater as compared to PVP. From the experimental dissolution results, it can be concluded that the formation of BCL ground complex with Ac-β-CD enhances the dissolution rate of the compound. The permeation was also shown to be advantageous in the presence of the polymers, which was demonstrated by the elevated fluxes of BCL through the membrane. The comparison of the dissolution/permeation processes was illustrated and discussed. The conclusion was made that the presence of HPMC as a stabilizer of the supersaturation state is promising and seems to be a useful tool for the optimization of BCL pharmaceutical formulations manufacturing.
A Quality by Design Approach in Pharmaceutical Development of Non-Viral Vectors with a Focus on miRNA
Authors: Toma Ioana , Porfire Alina Silvia , Tefas Lucia Ruxandra , Berindan-Neagoe Ioana , Tomuță Ioan
Published: July 2022

Cancer is the leading cause of death worldwide. Tumors consist of heterogeneous cell populations that have different biological properties. While conventional cancer therapy such as chemotherapy, radiotherapy, and surgery does not target cancer cells specifically, gene therapy is attracting increasing attention as an alternative capable of overcoming these limitations. With the advent of gene therapy, there is increasing interest in developing non-viral vectors for genetic material delivery in cancer therapy. Nanosystems, both organic and inorganic, are the most common non-viral vectors used in gene therapy. The most used organic vectors are polymeric and lipid-based delivery systems. These nanostructures are designed to bind and protect the genetic material, leading to high efficiency, prolonged gene expression, and low toxicity. Quality by Design (QbD) is a step-by-step approach that investigates all the factors that may affect the quality of the final product, leading to efficient pharmaceutical development. This paper aims to provide a new perspective regarding the use of the QbD approach for improving the quality of non-viral vectors for genetic material delivery and their application in cancer therapy.
Solvent Relaxation NMR as a Tool to Study Particle Dispersions in Non-Aqueous Systems
Authors: Alaei Zahra , Cattoz Beatrice , Dowding Peter John , Griffiths Peter Charles
Published: July 2022

The determination of the NMR spin–spin relaxation rate of water in (purely) aqueous particulate dispersions has been shown to be a convenient and facile experimental approach to probing the composition of near particle surface structures. Here, a systematic study has been undertaken of both non-aqueous and mixed aqueous–non-aqueous solvent particulate dispersions to explore the universality of the solvent relaxation technique. As in the aqueous case, a linear relationship between the surface area present and the solvent relaxation rate is observed, confirming the rapid exchange of the solvent molecules between the surface and the bulk and thereby illustrating the viability of the experimental methodology to study such systems. Crucially, the surface enhancement effect was considerably weaker in non-aqueous systems compared with aqueous dispersions and reflects a potential limitation of the wider deployment of this experimental methodology.
Magnesium Accumulation in Two Contrasting Varieties of Lycopersicum esculentum L. Fruits: Interaction with Calcium at Tissue Level and Implications on Quality
Authors: Pessoa Cláudia Campos , Lidon Fernando C. , Coelho Ana Rita F. , Marques Ana Coelho , Daccak Diana , Luís Inês Carmo , Caleiro João Cravidão , Kullberg José Carlos , Legoinha Paulo , Brito Maria Graça , Ramalho José Cochicho , Silva Maria José , Rodrigues Ana Paula , Guerra Mauro , Leitão Roberta G. , Campos Paula Scotti , Pais Isabel P. , Semedo José N. , Silva Maria Manuela , Galhano Carlos , Leal Nuno , Reboredo Fernando H. , Pessoa Maria Fernanda , Simões Manuela
Published: July 2022

As the productivity and quality of tomato fruits are responsive to Mg applications, without surpassing the threshold of toxicity, the assessment of potential levels of Mg accumulation in tissues, as well as the interactions with Ca and physicochemical properties, prompt this study. An agronomic workflow for Mg enrichment, consisting of six foliar applications of MgSO4 with four concentrations (0%, 0.25%, 1% and 4%), equivalent to 0, 43.9, 175.5 and 702 g ha−1, was applied on two tomato (Lycopersicum esculentum L.) genotypes (Heinz1534 and Heinz9205). During fruit development, leaf gas exchange was screened, with only minor physiological deviations being found. At harvest, Mg contents among tissues and the interactions with Ca were analyzed, and it was found that in both varieties a higher Mg/Ca ratio prevailed in the most external part of the fruit sprayed with 4% MgSO4. However, Mg distribution prevailed relatively near the epidermis in H1534, while in H9205 the higher contents of this nutrient occurred in the core of the fruit, which indicated a decrease of the relative proportion of Ca. The morphologic (height and diameter), physical (dry weight and density) and colorimetric parameters, and the total soluble solids of fruits, did not reveal significant changes in both tomato varieties. It was further concluded that foliar application until 4% MgSO4 does not have physiological impacts in the fruit’s quality of both varieties, but in spite of the different patterns of Mg accumulation in tissues, if the mean value in the whole fruit is considered, this nutrient prevails in H1534. This study thus suggests that variety H1534 can be used to attain tomato fruits with added value, providing an option of further processing to achieve food products with functional properties, ultimately proving a beneficial option to producers, the food processing industry and consumers. Moreover, the study reinforces the importance of variety choice when designing enrichment workflows.
Photosynthetic Responses, Growth, Production, and Tolerance of Traditional Varieties of Cowpea under Salt Stress
Authors: Praxedes Saulo Samuel Carneiro , Ferreira Neto Miguel , Loiola Aline Torquato , Santos Fernanda Jessica Queiroz , Umbelino Bianca Fernandes , Silva Luderlândio de Andrade , Moreira Rômulo Carantino Lucena , Melo Alberto Soares de , Lacerda Claudivan Feitosa de , Fernandes Pedro Dantas , Dias Nildo da Silva , Sá Francisco Vanies da Silva
Published: July 2022

Cowpea is the main subsistence crop—protein source—for the Brazilian semi-arid region. The use of salt-stress-tolerant varieties can improve crop yields. We evaluated the effect of irrigation with brackish water on the growth, photosynthetic responses, production, and tolerance of fifteen traditional varieties of cowpea. The experiment was conducted in randomized blocks, in a 15 × 2 factorial scheme, composed of 15 traditional varieties of cowpea and two salinity levels of irrigation water (0.5 and 4.5 dS m−1), with five replicates. Plants were grown in pots containing 10 dm3 of soil for 80 days. The reduction in the photosynthetic rate of cowpea varieties occurs mainly due to the decrease in stomatal conductance caused by salt stress. Salt stress increased the electron transport rate and photochemical quenching of cowpea varieties, but stress-tolerant varieties increased the CO2 assimilation rate and instantaneous carboxylation efficiency. The Ceará, Costela de Vaca, Pingo de Ouro, Ovo de Peru, and Sempre Verde varieties are tolerant to salt stress. Salt stress decreases 26% of the production of tolerant varieties to salt stress and 54% of susceptible varieties. The present findings show the existence of variability for saline stress tolerance in traditional varieties of cowpea and that Ceará, Costela de Vaca, Pingo de Ouro, and Ovo de Peru varieties are more suitable for crops irrigated with saline water.
Transcriptome Analysis Reveals Molecular Mechanisms under Salt Stress in Leaves of Foxtail Millet (Setaria italica L.)
Authors: Han Fei , Sun Mingjie , He Wei , Guo Shuqing , Feng Jingyi , Wang Hui , Yang Quangang , Pan Hong , Lou Yanhong , Zhuge Yuping
Published: July 2022

Foxtail millet (Setaria italica L.) is an important cereal for managing future water scarcity and ensuring food security, due to its strong drought and salt stress resistance owing to its developed root system. However, the molecular responses of foxtail millet leaves to salt stress are largely unknown. In this study, seeds of 104 foxtail millet accessions were subjected to 0.17 mol·L−1 NaCl stress during germination, and various germination-related parameters were analyzed to derive 5 salt-sensitive accessions and 13 salt-tolerant accessions. Hong Gu 2000 and Pu Huang Yu were the most salt-tolerant and salt-sensitive accessions, respectively. To determine the mechanism of the salt stress response, transcriptomic differences between the control and salt-treated groups were investigated. We obtained 2019 and 736 differentially expressed genes under salt stress in the salt-sensitive and salt-tolerant accessions, respectively. The transcription factor families bHLH, WRKY, AP2/ERF, and MYB-MYC were found to play critical roles in foxtail millet’s response to salt stress. Additionally, the down-regulation of ribosomal protein-related genes causes stunted growth in the salt-sensitive accessions. The salt-tolerant accession alleviates salt stress by increasing energy production. Our findings provide novel insights into the molecular mechanism of foxtail millet’s response to salt stress.
SlHSP17.7 Ameliorates Chilling Stress-Induced Damage by Regulating Phosphatidylglycerol Metabolism and Calcium Signal in Tomato Plants
Authors: Wu Yuanyuan , Lv Shuwen , Zhao Yaran , Chang Chenliang , Hong Wei , Jiang Jing
Published: July 2022

Tomatoes (Solanum lycopersicum L.) are sensitive to chilling temperatures between 0 °C and 12 °C owing to their tropical origin. SlHSP17.7, a cytoplasmic heat shock protein, interacts with cation/calcium exchanger 1-like (SlCCX1-like) protein and promotes chilling tolerance in tomato fruits (Zhang, et al., Plant Sci., 2020, 298, 1–12). The overexpression of SlHSP17.7 can also promote cold tolerance in tomato plants, but its specific mechanism remains unclear. In this study, we show that the overexpression of SlHSP17.7 in tomato plants enhances chilling tolerance with better activity of photosystem II (PSII). Metabolic analyses revealed that SlHSP17.7 improved membrane fluidity by raising the levels of polyunsaturated fatty acids. Transcriptome analyses showed that SlHSP17.7 activated Ca2+ signaling and induced the expression of C-repeat binding factor (CBF) genes, which in turn inhibited the production of reactive oxygen species (ROS). The gene coexpression network analysis showed that SlHSP17.7 is coexpressed with SlMED26b. SlMED26b silencing significantly lowered OE-HSP17.7 plants’ chilling tolerance. Thus, SlHSP17.7 modulates tolerance to chilling via both membrane fluidity and Ca2+-mediated CBF pathway in tomato plants.
Altered Developmental and Metabolic Gene Expression in Basil Interspecific Hybrids
Authors: Shah Saumya , Rastogi Shubhra , Vashisth Divya , Rout Prashant Kumar , Lal Raj Kishori , Lavania Umesh Chandra , Shasany Ajit Kumar
Published: July 2022

To understand the altered developmental changes and associated gene expression in inter-genomic combinations, a study was planned in two diverse yet closely related species of Ocimum, targeting their hybrid F1 and amphidiploids. The existing developmental variations between F1 and amphidiploids was analyzed through phenotypical and anatomical assessments. The absence of 8330 transcripts of F1 in amphidiploids and the exclusive presence of two transcripts related to WNK lysine-deficient protein kinase and geranylgeranyl transferase type-2 subunit beta 1-like proteins in amphidiploids provided a set of genes to compare the suppressed and activated functions between F1 and amphidiploids. The estimation of eugenol and methyleugenol, flavonoid, lignin and chlorophyll content was correlated with the average FPKM and differential gene expression values and further validated through qRT-PCR. Differentially expressed genes of stomatal patterning and development explained the higher density of stomata in F1 and the larger size of stomata in amphidiploids. Gene expression study of several transcription factors putatively involved in the growth and developmental processes of plants clearly amalgamates the transcriptome data linking the phenotypic differences in F1 and amphidiploids. This investigation describes the influence of interspecific hybridization on genes and transcription factors leading to developmental changes and alleviation of intergenomic instability in amphidiploids.
A Review of PRESAGE Radiochromic Polymer and the Compositions for Application in Radiotherapy Dosimetry
Authors: Mohyedin Muhammad Zamir , Zin Hafiz Mohd , Adenan Mohd Zulfadli , Abdul Rahman Ahmad Taufek
Published: July 2022

Recent advances in radiotherapy technology and techniques have allowed a highly conformal radiation to be delivered to the tumour target inside the body for cancer treatment. A three-dimensional (3D) dosimetry system is required to verify the accuracy of the complex treatment delivery. A 3D dosimeter based on the radiochromic response of a polymer towards ionising radiation has been introduced as the PRESAGE dosimeter. The polyurethane dosimeter matrix is combined with a leuco-dye and a free radical initiator, whose colour changes in proportion to the radiation dose. In the previous decade, PRESAGE gained improvement and enhancement as a 3D dosimeter. Notably, PRESAGE overcomes the limitations of its predecessors, the Fricke gel and the polymer gel dosimeters, which are challenging to fabricate and read out, sensitive to oxygen, and sensitive to diffusion. This article aims to review the characteristics of the radiochromic dosimeter and its clinical applications. The formulation of PRESAGE shows a delicate balance between the number of radical initiators, metal compounds, and catalysts to achieve stability, optimal sensitivity, and water equivalency. The applications of PRESAGE in advanced radiotherapy treatment verifications are also discussed.
Freestanding Activated Carbon Nanocomposite Electrodes for Capacitive Deionization of Water
Authors: Hussain Humair , Jilani Asim , Salah Numan , Alshahrie Ahmed , Memić Adnan , Ansari Mohammad Omaish , Dutta Joydeep
Published: July 2022

Freshwater reserves are being polluted every day due to the industrial revolution. Man-made activities have adverse effects upon the ecosystem. It is thus the hour of need to explore newer technologies to save and purify water for the growing human population. Capacitive deionization (CDI) is being considered as an emerging technique for removal of excess ions to produce potable water including desalination. Herein, cost-effective activated carbon incorporated with carbon nanotubes (CNT) was used as a freestanding electrode. Further, the desalination efficiency of the designed electrodes was tuned by varying binder concentration, i.e., polyvinylidene difluoride (PVDF) in the activated carbon powder and CNT mixture. PVDF concentration of 5, 7.5, 10, and 12.5 wt% was selected to optimize the freestanding electrode formation and further applied for desalination of water. PVDF content affected the surface morphology, specific surface area, and functional groups of the freestanding electrodes. Moreover, the electrical conductivity and specific surface area changed with PVDF concentration, which ultimately affected the desalination capacity using the freestanding electrodes. This study paves the way to produce cost effective carbon-based freestanding electrodes for capacitive deionization and other applications including battery electrodes.
Studying the Interfacial Properties of Carbon/Glass Hybrid Composites via the Nanoindentation Method
Authors: Jiang Xin , Gao Mingze , Zhu Jing , Ji Hongwei , Lang Fengchao
Published: July 2022

The mechanical properties of hybrid composite interfaces are critical in determining the overall properties of composite materials. To investigate the mechanical performance of hybrid composite interfaces, an accurate and efficient method must be developed. In this work, nanoindentation is used in this work to investigate the mechanical performance of the carbon/glass interface and the influence of the distance between carbon and the glass fibers on the modulus of the thermoset matrix. The results show that the interface sizes around the carbon and glass fibers are around 1.5 and 2.0 μm, respectively. The modulus around the carbon fibers is 5–11 GPa without the fiber effect, while that around the glass fibers is 4–10 GPa. The modulus of the matrix is not affected by the two types of fibers when the distance between them is greater than 4.5 μm.
Effect of TiO2 Nanoparticles on Capillary-Driven Flow in Water Nanofilters Based on Chitosan Cellulose and Polyvinylidene Fluoride Nanocomposites: A Theoretical Study
Authors: Mahdhi Noureddine , Alsaiari Norah Salem , Amari Abdelfattah , Chakhoum Mohamed Ali
Published: July 2022

In this study, a novel concept of nanofiltration process of drinking water based on capillary-driven nanofiltration is demonstrated using a bio-based nanocomposites’ nanofilter as free power: a green and sustainable solution. Based on Lifshitz and Young–Laplace theories, we show that the chitosan (CS), cellulose acetate (CLA), and Polyvinylidene fluoride (PVDF) polymer matrixes demonstrate hydrophobic behavior, which leads to the draining of water from nanopores when negative capillary pressure is applied and consequently prevents the capillary-driven nanofiltration process. By incorporating 10%, 20%, and 30% volume fraction of titanium dioxide (TiO2) nanoparticles (NPs) to the polymers’ matrixes, we demonstrate a wetting conversion from hydrophobic to hydrophilic behavior of these polymer nanocomposites. Subsequently, the threshold volume fraction of the TiO2 NPs for the conversion from draining (hydrophobic) to filling (hydrophilic) by capillary pressure were found to be equal to 5.1%, 10.9%, and 13.9%, respectively, for CS/TiO2, CLA/TiO2, and PVDF/TiO2 nanocomposites. Then, we demonstrated the negligible effect of the gravity force on capillary rise as well as the capillary-driven flow for nanoscale pore size. For nanofilters with the same effective nanopore radius, porosity, pore shape factor, and tortuosity, results from the modified Lucas–Washburn model show that the capillary rise as well as the capillary-driven water volume increase with increased volume fraction of the TiO2 NPs for all nanocomposite nanofilter. Interestingly, the capillary-driven water volume was in range (5.26–6.39) L/h·m2 with 30% volume fraction of TiO2 NPs, which support our idea for capillary-driven nanofiltration as zero energy consumption nano-filtration process. Correspondingly, the biodegradable CS/TiO2 and CLA/TiO2 nanocomposites nanofilter demonstrate capillary-driven water volume higher, ~1.5 and ~1.2 times, respectively, more than the synthetic PVDF/TiO2 nanocomposite.
Recent Developments in Tuning Methods for Predictive Functional Control
Authors: Rossiter John Anthony , Aftab Muhammad Saleheen
Published: July 2022

Predictive functional control (PFC) is a popular alternative to PID because it exploits model information better and enables systematic constraint handling while also being cheap and computationally efficient. A recent overview paper reviewed some recent proposals for improving the tuning efficacy. This paper extends and develops upon that review paper by introducing some exciting new proposals for how to making tuning more intuitive and, thus, easier for unskilled operators. Moreover, there are early indications that these proposals are easily modified for use in nonlinear cases while maintaining a very low cost and a simple and fast online computation.
Quantitative Assessment of the Efficacy of the Nd:YAG Laser Therapy of Psoriasis
Authors: Odrzywołek Wiktoria , Deda Anna , Zdrada Julita , Wcisło-Dziadecka Dominika , Błońska-Fajfrowska Barbara , Wilczyński Sławomir
Published: July 2022

Psoriasis is a chronic inflammatory disease. Within psoriatic lesions, microvascular dysfunction and numerous, tortuous blood vessels occurs. The Nd:YAG laser causes the selective destruction of dilated capillaries, which contributes to the inhibition of leukocyte transport within psoriatic lesions and may be an effective method of psoriasis therapy. This preliminary study was designed to assess the effectiveness of the Nd:YAG laser in reducing psoriatic lesions. Twenty volunteers suffering from psoriasis met the inclusion criteria and were qualified for the study. The non-lesional skin areas and areas with psoriatic lesions were imaged using a DUB SkinScanner high-frequency ultrasound, and skin parameters, including the stratum corneum hydration, melanin and hemoglobin level, were evaluated. The thickness of the epidermis and the SLEB layer, as well as the density of the dermis, were assessed before and after the therapy. The Nd:YAG laser, with a wavelength of 1064 nm and a 650-microsecond pulse duration, was used to treat psoriasis. The density of psoriatic skin after treatment increased by 7.26 u.m. and epidermis thickness decreased by 0.068 u.m. The stratum corneum hydration increased by 9.5 u.m., hemoglobin value decreased by 35 u.m. and melanin decreased by 12 u.m. The thickness of the SLEB layer was also reduced from 0.340 mm before the therapy to 0.195 mm after the therapy. The increase in skin density and the decrease in epidermal thickness after the therapy may prove the effectiveness of the Nd:YAG laser in reducing psoriatic lesions. The thickness of the SLEB layer and hemoglobin decrease indicate a reduction in inflammation. Laser therapy improves the epidermal hydration of psoriatic skin.
Muslim Instagram: Eternal Youthfulness and Cultivating Deen
Authors: Hasan Farah
Published: July 2022

This article argues that Muslims have created a specific Muslim Instagram that sustains youthfulness and cultivates their deen (religion). Instagram as a social has become a space for Muslim youth all over the world to share images. These images, being circulated over Instagram across localities, create visual representations for other users. For this research, over 500 images with the hashtags #muslim and #islam were analysed to understand how Muslims represent themselves and their religion online. A two-step methodological procedure involved the adaption of iconographical and iconological techniques of visual art interpretation to the images collected. The concept of youthfulness and the Islamic concept of deen will be discussed in relation to the analysed images to demonstrate the emergence of a Muslim Instagram. Muslim Instagram is a translocal space that enables Muslims to simultaneously act eternally youthful and cultivate their deen. By playing with notions of youthfulness, Muslims recontextualise their faith and practice online to cultivate their deen. They thereby embed Islam and subsume Islamic concepts and practices into modern global lifestyle patterns of consumption.
Reverse Sensitivity Analysis for Risk Modelling
Authors: Pesenti Silvana M.
Published: July 2022

We consider the problem where a modeller conducts sensitivity analysis of a model consisting of random input factors, a corresponding random output of interest, and a baseline probability measure. The modeller seeks to understand how the model (the distribution of the input factors as well as the output) changes under a stress on the output’s distribution. Specifically, for a stress on the output random variable, we derive the unique stressed distribution of the output that is closest in the Wasserstein distance to the baseline output’s distribution and satisfies the stress. We further derive the stressed model, including the stressed distribution of the inputs, which can be calculated in a numerically efficient way from a set of baseline Monte Carlo samples and which is implemented in the R package SWIM on CRAN. The proposed reverse sensitivity analysis framework is model-free and allows for stresses on the output such as (a) the mean and variance, (b) any distortion risk measure including the Value-at-Risk and Expected-Shortfall, and (c) expected utility type constraints, thus making the reverse sensitivity analysis framework suitable for risk models.
Evaluation of R Tools for Downloading MODIS Images and Their Use in Urban Growth Analysis of the City of Tarija (Bolivia)
Authors: Campero-Taboada Milton J. , Luquin Eduardo , Montesino-SanMartin Manuel , González-Audícana María , Campo-Bescós Miguel A.
Published: July 2022

The aim of this study was to compare the available tools in R for downloading and processing Moderate Resolution Imaging Spectroradiometer (MODIS) data, specifically the Enhanced Vegetation Index (EVI) product. The R tools evaluated were the MODIS package, RGISTools, MODISTools, R Google Earth Engine (RGEE) package, MODIStsp, and the Application for Extracting and Exploring Analysis Ready Samples (AppEEARS) application. Each tool was used to download the same product (EVI) corresponding to the same day (3 December 2015), and downloaded data were used to analyze the urban growth of Tarija (Bolivia) as an interesting application. The following features were analyzed: download time and memory used during the download, additional post-processing time, local memory occupied on the computer, and downloaded file formats. Results showed that the most efficient R tools were those that work directly in the “cloud” or use text queries (RGEE and AppEEARS, respectively) and provide, as a final product, a cropped.tif image according to the area of interest.
A Low-Complexity Underwater Acoustic Coherent Communication System for Small AUV
Authors: Jiang Weihua , Yang Xiaoyu , Tong Feng , Yang Yijun , Zhou Tianhua
Published: July 2022

While underwater acoustic (UWA) communication offers a practical way to establish a wireless link with underwater vehicles, designing a UWA communication system onboard a small autonomous underwater vehicle (AUV) still poses significant challenges. As the adoption of the low-complexity, robust noncoherent communication technology is limited by low bandwidth efficiency and a low data rate, coherent UWA communication requires Doppler mitigation and channel equalization measures to achieve a relatively high data rate in a moving state. Due to the strict constraints of a small-scale AUV in terms of resources and energy consumption, it is not appropriate to use high-complexity Doppler/multipath compensation technology from the prospect of system implementation. In this paper, an efficient and low-complexity UWA differential binary phase-shift keying (DBPSK) system onboard a small AUV is proposed by simplifying the Doppler and multipath compensation. Specifically, for Doppler, the delay of the adjacent DBPSK symbols is calculated according to the Doppler estimate to facilitate delay-tuning Doppler correction. For multipath, low-complexity LMS channel equalization is incorporated with error correction coding to enable multipath mitigation. With a simple structure and low computational complexity, the proposed scheme facilitates the practical hardware implementation and system integration in the small AUV platform. The numerical simulations are conducted to assess the validity of the proposed scheme under different channel conditions and the effectiveness of the proposed scheme is further verified by two UWA communication field tests, which are performed at a practical shallow water sea and lake, respectively.
An Efficient User-Friendly Integration Tool for Landslide Susceptibility Mapping Based on Support Vector Machines: SVM-LSM Toolbox
Authors: Huang Wubiao , Ding Mingtao , Li Zhenhong , Zhuang Jianqi , Yang Jing , Li Xinlong , Meng Ling’en , Zhang Hongyu , Dong Yue
Published: July 2022

Landslide susceptibility mapping (LSM) is an important element of landslide risk assessment, but the process often needs to span multiple platforms and the operation process is complex. This paper develops an efficient user-friendly toolbox including the whole process of LSM, known as the SVM-LSM toolbox. The toolbox realizes landslide susceptibility mapping based on a support vector machine (SVM), which can be integrated into the ArcGIS or ArcGIS Pro platform. The toolbox includes three sub-toolboxes, namely: (1) influence factor production, (2) factor selection and dataset production, and (3) model training and prediction. Influence factor production provides automatic calculation of DEM-related topographic factors, converts line vector data to continuous raster factors, and performs rainfall data processing. Factor selection uses the Pearson correlation coefficient (PCC) to calculate the correlations between factors, and the information gain ratio (IGR) to calculate the contributions of different factors to landslide occurrence. Dataset sample production includes the automatic generation of non-landslide data, data sample production and dataset split. The accuracy, precision, recall, F1 value, receiver operating characteristic (ROC) and area under curve (AUC) are used to evaluate the prediction ability of the model. In addition, two methods—single processing and multiprocessing—are used to generate LSM. The prediction efficiency of multiprocessing is much higher than that of the single process. In order to verify the performance and accuracy of the toolbox, Wuqi County, Yan’an City, Shaanxi Province was selected as the test area to generate LSM. The results show that the AUC value of the model is 0.8107. At the same time, the multiprocessing prediction tool improves the efficiency of the susceptibility prediction process by about 60%. The experimental results confirm the accuracy and practicability of the proposed toolbox in LSM.