bluuebunny/crossref_metadata_2025 huggingface dataset:
Sex Differences in Red Blood Cell Transfusions and 30-Day Mortality in Cardiac Surgery: A Single Center Observational Study
Authors: Räsänen Jenni , Ellam Sten , Hartikainen Juha , Juutilainen Auni , Halonen Jari
Published: December 2023

In cardiac surgery, women have higher short-term mortality and a higher risk of receiving red blood cell (RBC) transfusions than men. This study’s aim was to evaluate possible sex differences in RBC transfusions in cardiac surgery and their association with preoperative hemoglobin levels, body mass index, and 30-day mortality. A single-center retrospective study was conducted with 1583 patients (1181 men and 402 women) undergoing cardiac surgery. A total of 64.4% of the women and 33.0% of the men received an RBC transfusion. In a multivariable analysis, female sex was an independent predictor of RBC transfusion (OR 3.88, 95% CI 2.95–5.11, p < 0.001). Other independent predictors of RBC transfusion were age, preoperative hemoglobin level, and body mass index. The women were more likely to receive RBC transfusions than the men, regardless of the type of cardiac surgery. Decreased transfusion risk was found in all higher-than-normal weight categories in the women, but only in the severe obesity category in the men. Preoperative hemoglobin was similarly associated with RBC transfusion in the men and women. The crude 30-day mortality rate was higher in the women than in the men (2.5% vs. 0.9%, p = 0.018). In both sexes, RBC transfusion was associated with an increased probability of death within 30 days.
Innovative Experimental Ultrasound and US-Related Techniques Using the Murine Model in Pancreatic Ductal Adenocarcinoma: A Systematic Review
Authors: Coppola Andrea , Grasso Dario , Fontana Federico , Piacentino Filippo , Minici Roberto , Laganà Domenico , Ierardi Anna Maria , Carrafiello Gianpaolo , D’Angelo Fabio , Carcano Giulio , Venturini Massimo
Published: December 2023

Pancreatic ductal adenocarcinoma (PDAC) is a cancer with one of the highest mortality rates in the world. Several studies have been conductedusing preclinical experiments in mice to find new therapeutic strategies. Experimental ultrasound, in expert hands, is a safe, multifaceted, and relatively not-expensive device that helps researchers in several ways. In this systematic review, we propose a summary of the applications of ultrasonography in a preclinical mouse model of PDAC. Eighty-eight studies met our inclusion criteria. The included studies could be divided into seven main topics: ultrasound in pancreatic cancer diagnosis and progression (n: 21); dynamic contrast-enhanced ultrasound (DCE-US) (n: 5); microbubble ultra-sound-mediated drug delivery; focused ultrasound (n: 23); sonodynamic therapy (SDT) (n: 7); harmonic motion elastography (HME) and shear wave elastography (SWE) (n: 6); ultrasound-guided procedures (n: 9). In six cases, the articles fit into two or more sections. In conclusion, ultrasound can be a really useful, eclectic, and ductile tool in different diagnostic areas, not only regarding diagnosis but also in therapy, pharmacological and interventional treatment, and follow-up. All these multiple possibilities of use certainly represent a good starting point for the effective and wide use of murine ultrasonography in the study and comprehensive evaluation of pancreatic cancer.
Exercise Stress Echocardiography in Athletes: Applications, Methodology, and Challenges
Authors: Palermi Stefano , Sperlongano Simona , Mandoli Giulia Elena , Pastore Maria Concetta , Lisi Matteo , Benfari Giovanni , Ilardi Federica , Malagoli Alessandro , Russo Vincenzo , Ciampi Quirino , Cameli Matteo , D’Andrea Antonello
Published: December 2023

This comprehensive review explores the role of exercise stress echocardiography (ESE) in assessing cardiovascular health in athletes. Athletes often exhibit cardiovascular adaptations because of rigorous physical training, making the differentiation between physiological changes and potential pathological conditions challenging. ESE is a crucial diagnostic tool, offering detailed insights into an athlete’s cardiac function, reserve, and possible arrhythmias. This review highlights the methodology of ESE, emphasizing its significance in detecting exercise-induced anomalies and its application in distinguishing between athlete’s heart and other cardiovascular diseases. Recent advancements, such as LV global longitudinal strain (GLS) and myocardial work (MW), are introduced as innovative tools for the early detection of latent cardiac dysfunctions. However, the use of ESE also subsumes limitations and possible pitfalls, particularly in interpretation and potential false results, as explained in this article.
How Does Aphasia Affect Quality of Life? Preliminary Reports
Authors: Filipska-Blejder Karolina , Zielińska Jolanta , Zieliński Marek , Wiśniewski Adam , Ślusarz Robert
Published: December 2023

Aphasia leads to disability, which, in turn, limits and can result in a complete breakdown of roles and social bonds. Thus, the aim of this study was to assess the impact of aphasia disorders on the quality of life of patients after an ischemic stroke. A prospective study among 116 patients with an ischemic stroke (the study group: 68 participants, 38.25% female, included patients with aphasia after a stroke; the control group: 48 patients, 37.5% female, without aphasia) was conducted at the Neurological Department of the Provincial Specialist Hospital in Włocławek. The patients were assessed twice: the first assessment was conducted during hospitalization using the Aphasia Dynamics Assessment Scale and the Aphasia Test Method of Jadwiga Szumska, and the second assessment of the quality of life after six months was conducted using the WHOQOL-BREF questionnaire. The patients from the control group rated their overall quality of life more highly than the patients from the study group. Statistically significant differences were observed in the physical domain, the psychological domain, and the environmental domain. The analysis of sociodemographic factors indicated a negative impact on the quality of life of the following variables: female gender, 55–64 years of age, vocational education, and place of residence—rural area. The degree and type of aphasia influenced the overall quality of life. Studies with larger samples are necessary.
Predictive Value of the Interaction between CEA and Hemoglobin in Neoadjuvant CCRT Outcomes in Rectal Cancer Patients
Authors: Lai Yi-Hsuan , Chang Yu-Tien , Chang Yu-Jia , Tsai Jo-Ting , Li Ming-Hsien , Lin Jang-Chun
Published: December 2023

The adoption of neoadjuvant concurrent chemoradiotherapy (CCRT) has reshaped the therapeutic landscape, but response prediction remains challenging. This study investigates the interaction between pre-CCRT carcinoembryonic antigen (CEA) and post-CCRT hemoglobin (Hb) levels in predicting the response of locally advanced rectal cancer (LARC) to CCRT. Retrospective data from 93 rectal cancer patients receiving neoadjuvant CCRT were analyzed. Univariate analyses assessed clinical factors associated with tumor regression grade (TRG) and T-stage outcomes. Machine learning identified predictive biomarkers. Interaction effects between CEA and Hb were explored through subgroup analyses. Post-CCRT Hb varied between pre-CCRT CEA groups. The interaction between pre-CCRT CEA and post-CCRT Hb influenced TRG. Males with normal pre-CCRT CEA and anemia showed better treatment responses. Females with elevated pre-CCRT CEA and post-CCRT anemia exhibited poorer responses. The interaction effect between them was significant, indicating that their relationship with TRG was not additive. Inflammatory biomarkers, WBC, neutrophil count, and post-CCRT platelet level correlated with CCRT response. Contrasting with previous findings, anemia was a predictor of better treatment response in males with normal pre-CCRT CEA. The interaction between pre-CCRT CEA and post-CCRT Hb levels predicts the response of LARC to CCRT. CEA, Hb, and sex should be considered when assessing treatment response. Inflammatory biomarkers contribute to response prediction. Understanding these complex relationships can enhance personalized treatment approaches in rectal cancer patients.
New Antimicrobials and New Therapy Strategies for Endocarditis: Weapons That Should Be Defended
Authors: Oliva Alessandra , Cogliati Dezza Francesco , Cancelli Francesca , Curtolo Ambrogio , Falletta Antonio , Volpicelli Lorenzo , Venditti Mario
Published: December 2023

The overall low-quality evidence concerning the clinical benefits of different antibiotic regimens for the treatment of infective endocarditis (IE), which has made it difficult to strongly support or reject any regimen of antibiotic therapy, has led to a discrepancy between the available guidelines and clinical practice. In this complex scenario, very recently published guidelines have attempted to fill this gap. Indeed, in recent years several antimicrobials have entered the market, including ceftobiprole, ceftaroline, and the long-acting lipoglycopeptides dalbavancin and oritavancin. Despite being approved for different indications, real-world data on their use for the treatment of IE, alone or in combination, has accumulated over time. Furthermore, an old antibiotic, fosfomycin, has gained renewed interest for the treatment of complicated infections such as IE. In this narrative review, we focused on new antimicrobials and therapeutic strategies that we believe may provide important contributions to the advancement of Gram-positive IE treatment, providing a summary of the current in vitro, in vivo, and clinical evidence supporting their use in clinical practice.
Prognostic Relevance of Global Myocardial Work Index in Patients with Moderate Aortic Valve Stenosis
Authors: Kandels Joscha , Metze Michael , Hagendorff Andreas , Stöbe Stephan
Published: December 2023

Background: A reduced global myocardial work index (GWI) ≤ 1951 mmHg% is associated with increased mortality in patients with severe aortic valve stenosis (AS). However, parameters predicting the outcome in patients with moderate AS are limited. Therefore, the aim of this study was to evaluate the prognostic value of the GWI in patients with moderate AS. Methods and Results: In this prospective study, 103 patients with moderate AS (mean age 72 ± 10 years; male: 69%) underwent standardized transthoracic echocardiography. The primary endpoint was survival without an aortic valve replacement (AVR). After a median follow-up of 30 ± 5 months, 37 patients (36%) were referred for an AVR. Survival without an AVR was 96% at 12 months and 80% at 30 months (>1951 mmHg%) versus 96% and 68% (≤1951 mmHg%). A GWI ≤ 1951 mmHg% did not predict the need for an AVR (hazard ratio 1.31 (95% CI, 0.63–2.72), p = 0.49). Furthermore, there was no significant correlation between the mean GWI (1644 ± 448 mmHg%) and mean aortic valve pressure gradient (24.2 mmHg ± 6.2, p = 0.615) or effective aortic orifice area (1.24 cm2 ± 0.11, p = 0.678). There was no difference between the AVR and non-AVR groups in the occurrence of clinical symptoms. Conclusion: In contrast to patients with severe AS, a GWI ≤ 1951 mmHg% did not predict the need for an AVR. Further research is needed to improve the risk stratification in patients with moderate AS.
Severe Postoperative Pain in Total Knee Arthroplasty Patients: Risk Factors, Insights and Implications for Pain Management via a Digital Health Approach
Authors: Lebleu Julien , Pauwels Andries , Poilvache Hervé , Anract Philippe , Belbachir Anissa
Published: December 2023

Up to 25% of patients undergoing knee arthroplasty report chronic pain postoperatively. Early identification of high-risk individuals can enhance pain management strategies. This retrospective analysis investigates the incidence of severe postoperative pain and its associated risk factors among 740 patients who underwent total knee arthroplasty. Utilizing a digital application, patients provided comprehensive data encompassing pre- and postoperative pain levels, analgesic usage, and completed a chronic pain risk assessment. Participants were categorized into two distinct groups based on their pain status at three months post-op: Group D+ (14%), characterized by pain scores exceeding 40/100 and/or the utilization of level 2 or 3 analgesics, and Group D− (86%), who did not meet these criteria. An analysis of pain trajectories within these groups revealed a non-linear progression, with specific patterns emerging amongst those predisposed to chronic pain. Notably, patients with a trajectory towards chronic pain exhibited a plateau in pain intensity approximately three weeks post-surgery. Significant preoperative risk factors were identified, including elevated initial pain levels, the presence of comorbidities, pain in other body areas, heightened joint sensitivity and stiffness. This study highlights the utility of digital platforms in enhancing patient care, particularly through the continuous monitoring of pain. Such an approach facilitates the early identification of potential complications and enables timely interventions.
Clinical Predictors of Gastrointestinal Bleeding Source before Computed Tomography Angiography
Authors: Sbeit Wisam , Basheer Maamoun , Shahin Amir , Khoury Sharbel , Msheael Botros , Assy Nimer , Khoury Tawfik
Published: December 2023

Background: Acute gastrointestinal bleeding (GIB) is a commonly encountered medical emergency. In cases of negative endoscopic evaluations, computed tomography angiography (CTA) is usually the next diagnostic step. To date, data regarding positive CTA examinations are lacking. We aimed to assess the clinical and laboratory parameters that predict a positive CTA examination, as demonstrated by the extravasation of contrast material into the bowel lumen. Methods: We performed a single-center retrospective study, including all patients who were admitted with GIB and who underwent CTA. Analysis was performed to compare patients’ characteristics, and logistic regression was used to explore parameters associated with a positive CTA. Results: We included 154 patients. Of them, 25 patients (16.2%) had active GIB on CTA vs. 129 patients (83.8%) who did not. On univariate analysis, several parameters were positively associated with active GIB, including congestive heart failure (OR 2.47, 95% CI 1.04–5.86, p = 0.04), warfarin use (OR 4.76, 95% CI 1.49–15.21, p = 0.008), higher INR (OR 1.33, 1.04–1.69, p = 0.02), and low albumin level (OR 0.37, 95% CI 0.17–0.79, p = 0.01). On multivariate logistic regression analysis, only high INR (OR 1.34, 95% CI 1.02–1.76, p = 0.03) and low albumin (OR 0.3, 95% CI 0.12–0.7, p = 0.005) kept their positive association with active bleeding, while a high ASA score was negatively associated with an active GIB. Conclusions: We could identify high INR and low albumin as strong predictors of active GIB, as demonstrated by positive CTA. On the other hand, comorbid patients classified by a high ASA score did not experience a higher rate of active GIB.
Accelerometry in the Functional Assessment of Balance in People with Stroke: A Systematic Review
Authors: Pérez-López Juan Francisco , Cano-de-la-Cuerda Roberto , Ortiz-Gutiérrez Rosa María
Published: December 2023

Balance disturbances in people with lived experience of stroke affect activities of daily living and social participation, so assessing them is essential to know the level of functional independence. Accelerometers are electronic devices that allow kinematic variables of balance to be recorded and are a tool of great interest in the assessment of functional balance. To determine the validity and reliability of, as well as the most performed protocols using accelerometers in the functional assessment of balance in people with experience of stroke, a systematic search of articles published in the electronic databases PubMed, Scopus, the Web of Science, the Cochrane Library, the PEDro and the Virtual Health Library from Spain was performed following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines. We used QUADAS-2 to assess the quality of the included studies. Eight studies met the inclusion criteria, two studied reliability and validity, two studied reliability and four studied the validity of accelerometers in the assessment of balance in people with stroke. All studies indicated the kind of accelerometer, localization on the body, tests and outcome variables. The results indicate that accelerometers show excellent reliability values in the assessment of balance in people who had a prior stroke and disparate results in terms of validity. Triaxial accelerometers were most used, and the 4th and 5th lumbar and 1st and 2nd sacral vertebrae were the body areas most used for their placement.
Solute Clearance Evaluation and Filter Clotting Prediction in Continuous Renal Replacement Therapy
Authors: Yoshimoto Kohei , Matsuura Ryo , Komaru Yohei , Yoshida Teruhiko , Miyamoto Yoshihisa , Hamasaki Yoshifumi , Inokuchi Ryota , Nangaku Masaomi , Doi Kent
Published: December 2023

Unexpected filter clotting is a major problem in continuous renal replacement therapy (CRRT). Reduced solute clearance is observed prior to filter clotting. This single-center, retrospective, observational study aimed to determine whether reduced solute clearance of low- and medium-molecular-weight molecules in CRRT can predict filter clotting. Solute clearances of urea and myoglobin (Mb) were measured at 24 h after initiation of continuous hemodiafiltration (CHDF). Clearance per flow (CL/F) was calculated. The primary outcome was clotting of the filter in the subsequent 24 h, and 775 CHDF treatments conducted on 230 patients for at least 24 consecutive hours in our ICU were analyzed. Filter clotting was observed in 127 treatments involving 39 patients. Urea and Mb CL/F at 24 h were significantly lower in the patients who experienced clotting. Further analysis was limited to the first CHDF treatment of each patient to adjust for confounding factors. Multivariate logistic regression analysis revealed that both urea CL/F < 94% and Mb CL/F < 64% were significant predictors of clotting within the next 24 h. Lower urea and Mb CL/F measured at 24 h after CRRT initiation were associated with filter clotting in the next 24 h. Further study is necessary to ascertain whether measurement of urea and MB CL/F will help with avoiding unexpected filter clotting.
Angiographic Evaluation of the Feeding Artery in Skull Base Meningioma
Authors: Arima Hironori , Watanabe Yusuke , Tanoue Yuta , Morisako Hiroki , Kawakami Taichiro , Ichinose Tsutomu , Goto Takeo
Published: December 2023

To identify the characteristics of feeding arteries in skull base meningioma including location and prevalence, we evaluated the distributions and types of feeding arteries in skull base meningioma by cerebral angiography and assessed relationships to tumor attachment. We enrolled patients with skull base meningioma who underwent MRI and cerebral digital subtraction angiography (DSA), from September 2015 to October 2022. Subjects comprised 115 patients (32 males, 83 females; mean age, 52.7) with 117 meningiomas, showing tumor attachments around the “cavernous sinus to the upper part of the clivus” (Area 1), “lower part of the clivus to foramen magnum” (Area 2), and “tentorium around the petrous bone” (Area 3). Frequent arteries, such as the dorsal meningeal artery (DMA), the ascending pharyngeal artery (APA), the tentorial artery (TA), and the petrosal branch (PB) of the middle meningeal artery (MMA) were analyzed in terms of their associations with tumor attachment to Areas 1–3. Meningiomas with the DMA as a feeding artery correlated with tumor attachment to Area 1 (p < 0.001). Meningiomas with the APA correlated with tumor attachment to Area 2 (p < 0.001). Meningiomas with the TA correlated with tumor attachment to Area 3 (p < 0.001). The PB correlated with Area 3 (p < 0.05). Our study founded that visualization of these arteries correlated well with specific areas. These arteries were also the main feeders in each type of skull base meningioma.
Effect of Doping on Phase Formation in YBCO Composites
Authors: Tolendiuly Sanat , Sovet Aigerim , Fomenko Sergey
Published: December 2023

This article discusses an effective method for obtaining superconducting composites based on Y1Ba2Cu3O7-δ (YBCO) by optimizing the total preparation time in comparison with similar scientific works while searching for effective modifying micro-additives. YBCO-based composites were doped with microparticles of aluminum, nickel, and iron. It was established that the initial ratio of green components, heat treatment, and holding time directly affect the qualitative and quantitative formation of the useful superconducting phase Y123, which in turn affects the basic superconducting properties of the final material.
Coarse Alignment Methodology of Point Cloud Based on Camera Position/Orientation Estimation Model
Authors: Yoo Suhong , Kim Namhoon
Published: December 2023

This study presents a methodology for the coarse alignment of light detection and ranging (LiDAR) point clouds, which involves estimating the position and orientation of each station using the pinhole camera model and a position/orientation estimation algorithm. Ground control points are obtained using LiDAR camera images and the point clouds are obtained from the reference station. The estimated position and orientation vectors are used for point cloud registration. To evaluate the accuracy of the results, the positions of the LiDAR and the target were measured using a total station, and a comparison was carried out with the results of semi-automatic registration. The proposed methodology yielded an estimated mean LiDAR position error of 0.072 m, which was similar to the semi-automatic registration value of 0.070 m. When the point clouds of each station were registered using the estimated values, the mean registration accuracy was 0.124 m, while the semi-automatic registration accuracy was 0.072 m. The high accuracy of semi-automatic registration is due to its capability for performing both coarse alignment and refined registration. The comparison between the point cloud with refined alignment using the proposed methodology and the point-to-point distance analysis revealed that the average distance was measured at 0.0117 m. Moreover, 99% of the points exhibited distances within the range of 0.0696 m.
Biodiversity, Adaptation Strategies, and Opportunities in Extreme Marine Environments
Authors: Lauritano Chiara , Coppola Daniela
Published: December 2023

Extreme marine environments are peculiar ecosystems characterized by extremely variable and hostile physicochemical parameters [...]
Statistical Modeling and Applications of Joint Distributions for Significant Wave Height, Spectral Peak Period, and Peak Direction of Propagation: A Case Study in the Norwegian Sea
Authors: Pires Vieira Serta Clarissa , Haver Sverre , Li Lin
Published: December 2023

The estimation of long-term extreme response is a crucial task in the design of marine structures. The target extreme responses are typically defined by annual exceedance probabilities of 10−2 and 10−4. Various approaches can be employed for this purpose, with preference given to statistical long-term analysis, which involves aggregating the exceedance probabilities of all potential sea states contributing to the exceedance of the target extremes. A joint model encompassing important metocean parameters such as wind, waves, and current is often necessary. This study specifically focuses on waves and wave-induced responses. In characterizing short-term sea state conditions, significant wave height (Hs), spectral peak period (Tp) and peak direction of propagation (Φp) are identified as the most important sea state characteristics. The objective of this work is to present the results of the joint model for the three sea state parameters, i.e., Hs, Tp and Φp, at an offshore site in the Norwegian Sea. The conditional modeling approach is applied using long-term hindcast data, and different statistical models are discussed for fitting the marginal and conditional distributions. The fitted parameters for all directional sectors are provided, offering a comprehensive representation of the joint model for direct use in long-term response analysis. Two case studies are included to illustrate the application of the fitted joint model in long-term response analyses. The case studies identify the governing wave directions and the most important combinations of short-term sea state characteristics regarding the estimation of long-term extreme responses.
The Conservation and Study of Macromycetes in the Komarov Botanical Institute Basidiomycetes Culture Collection—Their Taxonomical Diversity and Biotechnological Prospects
Authors: Psurtseva Nadezhda V. , Kiyashko Anna A. , Senik Svetlana V. , Shakhova Natalya V. , Belova Nina V.
Published: December 2023

Culture collections (CCs) play an important role in the ex situ conservation of biological material and maintaining species and strains, which can be used for scientific and practical purposes. The Komarov Botanical Institute Basidiomycetes Culture Collection (LE-BIN) preserves a large number of original dikaryon strains of various taxonomical and ecological groups of fungi from different geographical regions. Started in the late 1950s for the investigation of Basidiomycetes’ biological activity, today, in Russia, it has become a unique specialized macromycetes collection, preserving 3680 strains from 776 species of fungi. The Collection’s development is aimed at ex situ conservation of fungal diversity, with an emphasis on preserving rare and endangered species, ectomycorrhizal fungi, and strains useful for biotechnology and medicine. The main methods applied in the collection for maintaining and working with cultures are described, and the results are presented. Some problems for the isolation and cultivation of species are discussed. The taxonomical structure and variety of the strains in the collection fund are analyzed, and they show that the taxonomical diversity of fungi in the LE-BIN is commensurable with the largest CCs in the world. The achievements from the ex situ conservation of the diversity of macromycetes and the main results from the screening and investigation of the collection’s strains demonstrate that a number of strains can be prospective producers of enzymes (oxidoreductases and proteases), lipids, and biologically active compounds (terpenoids, phthalides, etc.) for biotechnology and medicine.
A Multifaceted Approach to Developing an Australian National Map of Protected Cropping Structures
Authors: Clark Andrew , Shephard Craig , Robson Andrew , McKechnie Joel , Morrison R. Blake , Rankin Abbie
Published: December 2023

As the global population rises, there is an ever-increasing demand for food, in terms of volume, quality and sustainable production. Protected Cropping Structures (PCS) provide controlled farming environments that support the optimum use of crop inputs for plant growth, faster production cycles, multiple growing seasons per annum and increased yield, while offering greater control of pests, disease and adverse weather. Globally, there has been a rapid increase in the adoption of PCS. However, there remains a concerning knowledge gap in the availability of accurate and up-to-date spatial information that defines the extent (location and area) of PCS. This data is fundamental for providing metrics that inform decision making around forward selling, labour, processing and infrastructure requirements, traceability, biosecurity and natural disaster preparedness and response. This project addresses this need, by developing a national map of PCS for Australia using remotely sensed imagery and deep learning analytics, ancillary data, field validation and industry engagement. The resulting map presents the location and extent of all commercial glasshouses, polyhouses, polytunnels, shadehouses and permanent nets with an area of >0.2 ha. The outcomes of the project revealed deep learning techniques can accurately map PCS with models achieving F-Scores > 0.9 and accelerate the mapping where suitable imagery is available. Location-based tools supported by web mapping applications were critical for the validation of PCS locations and for building industry awareness and engagement. The final national PCS map is publicly available through an online dashboard which summarises the area of PCS structures at a range of scales including state/territory, local government area and individual structure. The outcomes of this project have set a global standard on how this level of mapping can be achieved through a collaborative, multifaceted approach.
Vegetation Fires in the Lubumbashi Charcoal Production Basin (The Democratic Republic of the Congo): Drivers, Extent and Spatiotemporal Dynamics
Authors: Useni Sikuzani Yannick , Mpanda Mukenza Médard , Khoji Muteya Héritier , Cirezi Cizungu Nadège , Malaisse François , Bogaert Jan
Published: December 2023

In the Lubumbashi charcoal production basin (LCPB) in Southeastern DR Congo, agricultural and charcoal production activities regularly give rise to fires that lead to considerable degradation of the miombo open forest. This study analyzes the drivers of the spatiotemporal distribution of active fires and burnt areas in the LCPB by processing MODIS and Landsat data. In addition, a kernel density analysis method (KDE) was used to estimate fire risk, while the effect of the road network and dwellings on vegetation fires was highlighted in areas between a 0 and 3000 m radius. The obtained results revealed that fires in the LCPB generally occur between April and November, mainly during the day, between 11 a.m. and 12 p.m. These fires are concentrated in the central and southwestern part of the LCPB, more specifically in the savannahs and near roads. From 2002 to 2022, an average of 11,237 active fires and an average of 6337 km2 of burnt areas were recorded in the LCPB. Each year, these fires peak in August, and despite their steady decline, the few fires that have affected the forests have caused more devastation (more than 2790 km2/year) than those observed in the fields and savannah. These figures highlight the imperative need to put in place fire prevention and management measures in the LCPB, with particular emphasis on awareness, monitoring, and fire-fighting measures.
Role of Silver Nitrate Spray for Skin Wound Care in Patients with Toxic Epidermal Necrolysis: Our Experience in 4 Patients
Authors: Martinez Jose Dario , Cardenas Jesus Alberto , Soria Manuel , Saenz Luis Manuel , Estrada Kattya , Delgado Sergio Maximo , Ionescu Marius-Anton , Busila Camelia , Tatu Alin Laurentiu
Published: December 2023

Stevens–Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are examples of severe cutaneous adverse reactions to drugs (SCARs) with several international recommendations for global medical management, ranging from pharmacological systemic therapy to skin wound care. There is no defined best management of the skin wounds in SJS/TEN. The care of wounds is essential to initiate re-epithelialization. Our objective is to improve the cicatrization process, avoiding scarring due to deepening of the wounds, as well as prevent infections, achieve pain control, and avoid loss of serum proteins, fluids, and electrolytes. In this retrospective case series, we highlight the value of systemic therapy and the use of silver nitrate for wound management in four patients with TEN.
Psilocybin and Eugenol Reduce Inflammation in Human 3D EpiIntestinal Tissue
Authors: Robinson Gregory Ian , Li Dongping , Wang Bo , Rahman Tahiat , Gerasymchuk Marta , Hudson Darryl , Kovalchuk Olga , Kovalchuk Igor
Published: December 2023

Inflammation plays a pivotal role in the development and progression of inflammatory bowel disease (IBD), by contributing to tissue damage and exacerbating the immune response. The investigation of serotonin receptor 2A (5-HT2A) ligands and transient receptor potential (TRP) channel ligands is of significant interest due to their potential to modulate key inflammatory pathways, mitigate the pathological effects of inflammation, and offer new avenues for therapeutic interventions in IBD. This study investigates the anti-inflammatory effects of 5-HT2A ligands, including psilocybin, 4-AcO-DMT, and ketanserin, in combination with TRP channel ligands, including capsaicin, curcumin, and eugenol, on the inflammatory response induced by tumor necrosis factor (TNF)-α and interferon (IFN)-γ in human 3D EpiIntestinal tissue. Enzyme-linked immunosorbent assay was used to assess the expression of pro-inflammatory markers TNF-α, IFN-γ, IL-6, IL-8, MCP-1, and GM-CSF. Our results show that psilocybin, 4-AcO-DMT, and eugenol significantly reduce TNF-α and IFN-γ levels, while capsaicin and curcumin decrease these markers to a lesser extent. Psilocybin effectively lowers IL-6 and IL-8 levels, but curcumin, capsaicin, and 4-AcO-DMT have limited effects on these markers. In addition, psilocybin can significantly decrease MCP-1 and GM-CSF levels. While ketanserin lowers IL-6 and GM-CSF levels, there are no effects seen on TNF-α, IFN-γ, IL-8, or MCP-1. Although synergistic effects between 5-HT2A and TRP channel ligands are minimal in this study, the results provide further evidence of the anti-inflammatory effects of psilocybin and eugenol. Further research is needed to understand the mechanisms of action and the feasibility of using these compounds as anti-inflammatory therapies for conditions like IBD.
Dissecting the Neuronal Contributions of the Lipid Regulator NHR-49 Function in Lifespan and Behavior in C. elegans
Authors: Kwon Saebom , Park Kyu-Sang , Yoon Kyoung-hye
Published: December 2023

Although the importance of lipid homeostasis in neuronal function is undisputed, how they are regulated within neurons to support their unique function is an area of active study. NHR-49 is a nuclear hormone receptor functionally similar to PPARα, and a major lipid regulator in C. elegans. Although expressed in most tissues, little is known about its roles outside the intestine, the main metabolic organ of C. elegans. Here, using tissue- and neuron-type-specific transgenic strains, we examined the contribution of neuronal NHR-49 to cell-autonomous and non-autonomous nhr-49 mutant phenotypes. We examined lifespan, brood size, early egg-laying, and reduced locomotion on food. We found that lifespan and brood size could be rescued by neuronal NHR-49, and that NHR-49 in cholinergic and serotonergic neurons is sufficient to restore lifespan. For behavioral phenotypes, NHR-49 in serotonergic neurons was sufficient to control egg-laying, whereas no single tissue or neuron type was able to rescue the enhanced on-food slowing behavior. Our study shows that NHR-49 can function in single neuron types to regulate C. elegans physiology and behavior, and provides a platform to further investigate how lipid metabolism in neurons impact neuronal function and overall health of the organism.
Chronic Nonbacterial Osteomyelitis in Inflammatory Bowel Disease
Authors: Tzaneti Ariadni , Athanasopoulou Elli , Fessatou Smaragdi , Fotis Lampros
Published: December 2023

Chronic nonbacterial osteomyelitis (CNO), also known as chronic recurrent multifocal osteomyelitis (CRMO), is a rare autoinflammatory bone disease primarily affecting children and adolescents. This review presents a comprehensive analysis of the intricate relationship between CNO and inflammatory bowel disease (IBD), shedding light on shared pathophysiological mechanisms and clinical management. A thorough literature review was conducted, encompassing 24 case reports involving 40 patients. The demographic distribution of patients revealed a near-equal gender ratio, with a median age of diagnosis at 12 years. The diagnosis patterns showed a higher proportion of CNO as the initial diagnosis, while Crohn’s disease was more prevalent than ulcerative colitis. The time interval between the clinical presentations varied, ranging from simultaneous detection to a substantial 15-year gap. Treatment modalities included nonsteroidal anti-inflammatory drugs (NSAIDs), steroids, aminosalicylates, and biologic agents, such as infliximab, often overlapping in their use and suggesting shared pathophysiological pathways. Both conditions displayed systemic manifestations, and patients often responded well to immunosuppressive medications. The pathophysiology of CNO involves a genetic predisposition, cytokine dysregulation, and osteoclast activation. Dysregulated innate immunity results in immune cell infiltration into bones, causing sterile bone lesions. Notably, emerging evidence hints at a potential link between the microbiome and CNO. In contrast, IBD results from imbalanced mucosal immune responses to the intestinal microbiota. Polymorphisms in the promotor region of IL-10, common cytokines, immune cells, and genetic markers indicate shared immunological and genetic factors between CNO and IBD. Both conditions also involve extraintestinal symptoms. This analysis underscores the need for clinical awareness of the co-occurrence of CNO and IBD, especially among pediatric patients. A deepened understanding of the connections between these seemingly distinct diseases could lead to more effective management and improved patient outcomes.
The Clinical Application of Platelet-Rich Plasma in the Female Reproductive System: A Narrative Review
Authors: Vali Saaliha , Saso Srdjan , Bracewell Milnes Timothy , Nicopoullos James , Thum Meen-Yau , Smith James Richard , Jones Benjamin P.
Published: December 2023

Platelet-rich plasma is an autologous plasma containing platelets prepared from fresh whole blood drawn from a peripheral vein. Through processing, it can be prepared to contain supraphysiologic levels of platelets at three to five times greater than the level of normal plasma. PRP has been explored both in vivo and ex vivo in the human endometrium model in its ability to harness the intrinsic regenerative capacity of the endometrium. Intrauterine autologous PRP infusions have been shown to increase endometrial thickness and reduce the rate of intrauterine adhesions. In the setting of recurrent implantation failure, intrauterine infusion of PRP has been shown to increase clinical pregnancy rate. PRP also appears to hold a potential role in select patients with premature ovarian insufficiency, poor ovarian responders and in improving outcomes following frozen–thawed transplantation of autologous ovarian tissue. Further studies are required to explore the potential role of PRP in reproductive medicine further, to help standardise PRP protocols and evaluate which routes of administration are most effective.
Mineral Indicators of Geologically Recent Past Habitability on Mars
Authors: Hart Roger , Cardace Dawn
Published: December 2023

We provide new support for habitable microenvironments in the near-subsurface of Mars, hosted in Fe- and Mg-rich rock units, and present a list of minerals that can serve as indicators of specific water–rock reactions in recent geologic paleohabitats for follow-on study. We modeled, using a thermodynamic basis without selective phase suppression, the reactions of published Martian meteorites and Jezero Crater igneous rock compositions and reasonable planetary waters (saline, alkaline waters) using Geochemist’s Workbench Ver. 12.0. Solid-phase inputs were meteorite compositions for ALH 77005, Nakhla, and Chassigny, and two rock units from the Mars 2020 Perseverance rover sites, Máaz and Séítah. Six plausible Martian groundwater types [NaClO4, Mg(ClO4)2, Ca(ClO4)2, Mg-Na2(ClO4)2, Ca-Na2(ClO4)2, Mg-Ca(ClO4)2] and a unique Mars soil-water analog solution (dilute saline solution) named “Rosy Red”, related to the Phoenix Lander mission, were the aqueous-phase inputs. Geophysical conditions were tuned to near-subsurface Mars (100 °C or 373.15 K, associated with residual heat from a magmatic system, impact event, or a concentration of radionuclides, and 101.3 kPa, similar to <10 m depth). Mineral products were dominated by phyllosilicates such as serpentine-group minerals in most reaction paths, but differed in some important indicator minerals. Modeled products varied in physicochemical properties (pH, Eh, conductivity), major ion activities, and related gas fugacities, with different ecological implications. The microbial habitability of pore spaces in subsurface groundwater percolation systems was interrogated at equilibrium in a thermodynamic framework, based on Gibbs Free Energy Minimization. Models run with the Chassigny meteorite produced the overall highest H2 fugacity. Models reliant on the Rosy Red soil-water analog produced the highest sustained CH4 fugacity (maximum values observed for reactant ALH 77005). In general, Chassigny meteorite protoliths produced the best yield regarding Gibbs Free Energy, from an astrobiological perspective. Occurrences of serpentine and saponite across models are key: these minerals have been observed using CRISM spectral data, and their formation via serpentinization would be consistent with geologically recent-past H2 and CH4 production and sustained energy sources for microbial life. We list index minerals to be used as diagnostic for paleo water–rock models that could have supported geologically recent-past microbial activity, and suggest their application as criteria for future astrobiology study-site selections.
Point-Contact Spectroscopy in Bulk Samples of Electron-Doped Cuprate Superconductors
Authors: Nigro Angela , Guarino Anita , Leo Antonio , Grimaldi Gaia , Avitabile Francesco , Romano Paola
Published: December 2023

Point-contact spectroscopy was performed on bulk samples of electron-doped high temperature superconductor Nd2−xCexCuO4−δ. The samples were characterized using X-ray diffraction and scanning electron microscopy equipped with a wavelength-dispersive spectrometer and an electron backscatter diffraction detector. Samples with Ce content x = 0.15 showed the absence of spurious phases and randomly oriented grains, most of which had dimensions of approximately 220 µm2. The low-bias spectra in the tunneling regime, i.e., high-transparency interface, exhibited a gap feature at about ±5 meV and no zero-bias conductance, despite the random oriented grains investigated within our bulk samples, consistent with most of the literature data on oriented samples. High-bias conductance was also measured in order to obtain information on the properties of the barrier. A V-shape was observed in some cases, instead of the parabolic behavior expected for tunnel junctions.
Growth and Dispersion Control of SnO2 Nanocrystals Employing an Amino Acid Ester Hydrochloride in Solution Synthesis: Microstructures and Photovoltaic Applications
Authors: Hattori Nagisa , Vafaei Saeid , Narita Ryoki , Nagaya Naohide , Yoshida Norimitsu , Sugiura Takashi , Manseki Kazuhiro
Published: December 2023

Tin oxide (SnO2) is a technologically important semiconductor with versatile applications. In particular, attention is being paid to nanostructured SnO2 materials for use as a part of the constituents in perovskite solar cells (PSCs), an emerging renewable energy technology. This is mainly because SnO2 has high electron mobility, making it favorable for use in the electron transport layer (ETL) in these devices, in which SnO2 thin films play a role in extracting electrons from the adjacent light-absorber, i.e., lead halide perovskite compounds. Investigation of SnO2 solution synthesis under diverse reaction conditions is crucial in order to lay the foundation for the cost-effective production of PSCs. This research focuses on the facile catalyst-free synthesis of single-nanometer-scale SnO2 nanocrystals employing an aromatic organic ligand (as the structure-directing agent) and Sn(IV) salt in an aqueous solution. Most notably, the use of an aromatic amino acid ester hydrochloride salt—i.e., phenylalanine methyl ester hydrochloride (denoted as L hereafter)—allowed us to obtain an aqueous precursor solution containing a higher concentration of ligand L, in addition to facilitating the growth of SnO2 nanoparticles as small as 3 nm with a narrow size distribution, which were analyzed by means of high-resolution transmission electron microscopy (HR-TEM). Moreover, the nanoparticles were proved to be crystallized and uniformly dispersed in the reaction mixture. The environmentally benign, ethanol-based SnO2 nanofluids stabilized with the capping agent L for the Sn(IV) ions were also successfully obtained and spin-coated to produce a SnO2 nanoparticle film to serve as an ETL for PSCs. Several SnO2 ETLs that were created by varying the temperature of nanoparticle synthesis were examined to gain insight into the performance of PSCs. It is thought that reaction conditions that utilize high concentrations of ligand L to control the growth and dispersion of SnO2 nanoparticles could serve as useful criteria for designing SnO2 ETLs, since hydrochloride salt L can offer significant potential as a functional compound by controlling the microstructures of individual SnO2 nanoparticles and the self-assembly process to form nanostructured SnO2 thin films.
Advanced 3D Through-Si-Via and Solder Bumping Technology: A Review
Authors: Jang Ye Jin , Sharma Ashutosh , Jung Jae Pil
Published: December 2023

Three-dimensional (3D) packaging using through-Si-via (TSV) is a key technique for achieving high-density integration, high-speed connectivity, and for downsizing of electronic devices. This paper describes recent developments in TSV fabrication and bonding methods in advanced 3D electronic packaging. In particular, the authors have overviewed the recent progress in the fabrication of TSV, various etching and functional layers, and conductive filling of TSVs, as well as bonding materials such as low-temperature nano-modified solders, transient liquid phase (TLP) bonding, Cu pillars, composite hybrids, and bump-free bonding, as well as the role of emerging high entropy alloy (HEA) solders in 3D microelectronic packaging. This paper serves as a guideline enumerating the current developments in 3D packaging that allow Si semiconductors to deliver improved performance and power efficiency.
Influence of Strontium on the Biological Behavior of Bioactive Glasses for Bone Regeneration
Authors: Silva Amanda Vieira , Gomes Déborah dos Santos , Victor Rayssa de Sousa , Santana Lisiane Navarro de Lima , Neves Gelmires Araújo , Menezes Romualdo Rodrigues
Published: December 2023

Bioactive glasses (BGs) can potentially be applied in biomedicine, mainly for bone repair and replacement, given their unique ability to connect to natural bone tissue and stimulate bone regeneration. Since their discovery, several glass compositions have been developed to improve the properties and clinical abilities of traditional bioactive glass. Different inorganic ions, such as strontium (Sr2+), have been incorporated in BG due to their ability to perform therapeutic functions. Sr2+ has been gaining prominence due to its ability to stimulate osteogenesis, providing an appropriate environment to improve bone regeneration, in addition to its antibacterial potential. However, as there are still points in the literature that are not well consolidated, such as the influence of ionic concentrations and the BG production technique, this review aims to collect information on the state of the art of the biological behavior of BGs containing Sr2+. It also aims to gather data on different types of BGs doped with different concentrations of Sr2+, and to highlight the manufacturing techniques used in order to analyze the influence of the incorporation of this ion for bone regeneration purposes.