bluuebunny/crossref_metadata_2025 huggingface dataset:
Microstructural Evolution of Amphibole Peridotites in Åheim, Norway, and the Implications for Seismic Anisotropy in the Mantle Wedge
Authors: Jung Sejin , Jung Haemyeong , Austrheim Håkon
Published: April 2020

The microstructure of amphibole peridotites from Åheim, Norway were analyzed to understand the evolution of the lattice-preferred orientation (LPO) of olivine throughout the Scandian Orogeny and its implication for the seismic anisotropy of the subduction zone. The Åheim peridotites had a porphyroclastic texture and some samples contained an abundant amount of hydrous minerals such as tremolite. Detailed microstructural analysis on the Åheim peridotites revealed multiple stages of deformation. The coarse grains showed an A-type LPO of olivine, which can be interpreted as the initial stage of deformation. The spinel-bearing samples showed a mixture of B-type and C-type LPOs of olivine, which is considered to represent the deformation under water-rich conditions. The recrystallized fine-grained olivine displays a B-type LPO, which can be interpreted as the final stage of deformation. Microstructures and water content of olivine indicate that the dominant deformation mechanism of olivine showing a B-type LPO is a dislocation creep under water-rich condition. The observation of the B-type LPO of olivine is important for an interpretation of trench-parallel seismic anisotropy in the mantle wedge. The calculated seismic anisotropy of the tremolite showed that tremolite can contribute to the trench-parallel seismic anisotropy in the mantle wedge.
Porous Gold: A New Frontier for Enzyme-Based Electrodes
Authors: Bollella Paolo
Published: April 2020

Porous gold (PG) layers modified electrodes have emerged as valuable enzyme support to realize multiple enzyme-based bioelectrochemical devices like biosensors, enzymatic fuel cells (EFCs), smart drug delivery devices triggered by enzyme catalyzed reactions, etc. PG films can be synthesized by using different methods such as dealloying, electrochemical (e.g., templated electrochemical deposition, self-templated electrochemical deposition, etc.) self-assembly and sputter deposition. This review aims to summarize the recent findings about PG synthesis and electrosynthesis, its characterization and application for enzyme-based electrodes used for biosensors and enzymatic fuel cells (EFCs) development.
Ligand-Assisted Formation of Graphene/Quantum Dot Monolayers with Improved Morphological and Electrical Properties
Authors: Litvin Aleksandr P. , Babaev Anton A. , Parfenov Peter S. , Dubavik Aliaksei , Cherevkov Sergei A. , Baranov Mikhail A. , Bogdanov Kirill V. , Reznik Ivan A. , Ilin Pavel O. , Zhang Xiaoyu , Purcell-Milton Finn , Gun’ko Yurii K. , Fedorov Anatoly V. , Baranov Alexander V.
Published: April 2020

Hybrid nanomaterials based on graphene and PbS quantum dots (QDs) have demonstrated promising applications in optoelectronics. However, the formation of high-quality large-area hybrid films remains technologically challenging. Here, we demonstrate that ligand-assisted self-organization of covalently bonded PbS QDs and reduced graphene oxide (rGO) can be utilized for the formation of highly uniform monolayers. After the post-deposition ligand exchange, these films demonstrated high conductivity and photoresponse. The obtained films demonstrate a remarkable improvement in morphology and charge transport compared to those obtained by the spin-coating method. It is expected that these materials might find a range of applications in photovoltaics and optoelectronics.
High Surface Area Nanoporous Graphitic Carbon Materials Derived from Lapsi Seed with Enhanced Supercapacitance
Authors: Shrestha Lok Kumar , Shrestha Rekha Goswami , Maji Subrata , Pokharel Bhadra P. , Rajbhandari Rinita , Shrestha Ram Lal , Pradhananga Raja Ram , Hill Jonathan P. , Ariga Katsuhiko
Published: April 2020

Nanoporous activated carbon materials derived from agro-wastes could be suitable low-cost electrode materials for high-rate performance electrochemical supercapacitors. Here we report high surface area nanoporous carbon materials derived from Lapsi seed agro-waste prepared by zinc chloride (ZnCl2) activation at 700 °C. Powder X-ray diffraction (pXRD) and Raman scattering confirmed the amorphous structure of the resulting carboniferous materials, which also incorporate oxygen-containing functional groups as confirmed by Fourier transform infrared (FTIR) spectroscopy. Scanning and transmission electron microscopy (SEM and TEM) analyses revealed the granular, nanoporous structures of the materials. High-resolution TEM (HR-TEM) confirmed a graphitic carbon structure containing interconnected mesopores. Surface areas and pore volumes of the materials were found, respectively, in the ranges from 931 to 2272 m2 g−1 and 0.998 to 2.845 cm3 g−1, and are thus superior to commercially available activated carbons. High surface areas, large pore volumes and interconnected mesopore structures of these Lapsi seed-derived nanoporous carbon materials lead to their excellent electrochemical supercapacitance performance in aqueous electrolyte (1 M H2SO4) with a maximum specific capacitance of 284 F g−1 at a current density of 1 A g−1. Furthermore, the electrodes showed high-rate capability sustaining 67.7% capacity retention even at high current density of 20 A g−1 with excellent cycle stability achieving 99% capacitance retention even after 10,000 charge–discharge cycles demonstrating the potential of Lapsi seed derived nanoporous carbons as suitable electrode materials in high-performance supercapacitor devices.
Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry
Authors: Rovera Cesare , Fiori Filippo , Trabattoni Silvia , Romano Diego , Farris Stefano
Published: April 2020

Bacterial cellulose nanocrystals (BCNCs) obtained by enzymatic hydrolysis have been loaded in pullulan biopolymer for use as nanoparticles in the generation of high-oxygen barrier coatings intended for food packaging applications. Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was hydrolyzed by two different enzymatic treatments, i.e., using endo-1,4-β-glucanases (EGs) from Thermobifida halotolerans and cellulase from Trichoderma reesei. The hydrolytic activity was compared by means of turbidity experiments over a period of 145 h, whereas BCNCs in their final state were compared, in terms of size and morphology, by atomic force microscopy (AFM) and dynamic light scattering (DLS). Though both treatments led to particles of similar size, a greater amount of nano-sized particles (≈250 nm) were observed in the system that also included cellulase enzymes. Unexpectedly, transmission electron microscopy (TEM) revealed that cellulose nanoparticles were round-shaped and made of 4–5 short (150–180 nm) piled whiskers. Pullulan/BCNCs nanocomposite coatings allowed an increase in the overall oxygen barrier performance, of more than two and one orders of magnitude (≈0.7 mL·m−2·24 h−1), of pure polyethylene terephthalate (PET) (≈120 mL·m−2·24 h−1) as well as pullulan/coated PET (≈6 mL·m−2·24 h−1), with no significant difference between treatments (hydrolysis mediated by EGs or with the addition of cellulase). BCNCs obtained by enzymatic hydrolysis have the potential to generate high oxygen barrier coatings for the food packaging industry.
Droplet Coalescence by Selective Wettability Enhancement in Microfluidic Devices
Authors: Alamoodi Nahla , Alazzam Anas
Published: April 2020

A new approach for droplet coalescence in microfluidic channels based on selective surface energy alteration is demonstrated. The proposed method involves patterning the surface of cyclic olefin copolymer (COC), a hydrophobic substrate attached to a polydimethylsiloxane hydrophobic microchannel, with graphene oxide (GO) using standard microfabrication techniques. Surface wettability and adhesion analyses confirmed the enhancement of the COC surface energy upon GO patterning and the stability of the GO film on COC. Three representative cases are illustrated to demonstrate the effectiveness of the method on the coalescence of droplets for different droplet flow regimes, as well as the effect of changing the size of the patterned surface area on the fusion process. The method achieves droplet coalescence without the need for precise synchronization.
Micromagnetic Simulations of Chaotic Ferromagnetic Nanofiber Networks
Authors: Blachowicz Tomasz , Döpke Christoph , Ehrmann Andrea
Published: April 2020

Electrospinning can be used to create nanofibers with diameters of typically a few tens to a few hundred nanometers. While pure polymers are often electrospun, it is also possible to use polymer blends or to include nanoparticles. In this way, e.g., magnetic nanofiber networks can be created with a certain diameter distribution, random fiber orientations, and random crossing positions and angles. Here we present for the first time micromagnetic simulations of small parts of stochastically oriented nanofiber networks. Magnetization reversal mechanisms are investigated for different local spatial distributions; mutual influences of neighboring magnetic fibers due to dipolar interactions are depicted. This study serves as a base for the possible use of such stochastic nanofiber networks in the research area of neuro-inspired materials.
Effective Platinum-Copper Catalysts for Methanol Oxidation and Oxygen Reduction in Proton-Exchange Membrane Fuel Cell
Authors: Menshchikov Vladislav , Alekseenko Anastasya , Guterman Vladimir , Nechitailov Andrey , Glebova Nadezhda , Tomasov Aleksandr , Spiridonova Olga , Belenov Sergey , Zelenina Natalia , Safronenko Olga
Published: April 2020

The behavior of supported alloyed and de-alloyed platinum-copper catalysts, which contained 14–27% wt. of Pt, was studied in the reactions of methanol electrooxidation (MOR) and oxygen electroreduction (ORR) in 0.1 M HClO4 solutions. Alloyed PtCux/C catalysts were prepared by a multistage sequential deposition of copper and platinum onto a Vulcan XC72 dispersed carbon support. De-alloyed PtCux−y/C catalysts were prepared by PtCux/C materials pretreatment in acid solutions. The effects of the catalysts initial composition and the acid treatment condition on their composition, structure, and catalytic activity in MOR and ORR were studied. Functional characteristics of platinum-copper catalysts were compared with those of commercial Pt/C catalysts when tested, both in an electrochemical cell and in H2/Air membrane-electrode assembly (MEA). It was shown that the acid pretreatment of platinum-copper catalysts practically does not have negative effect on their catalytic activity, but it reduces the amount of copper passing into the solution during the subsequent electrochemical study. The activity of platinum-copper catalysts in the MOR and the current-voltage characteristics of the H2/Air proton-exchange membrane fuel cell MEAs measured in the process of their life tests were much higher than those of the Pt/C catalysts.
Green Tea Polyphenol EGCG Attenuates MDSCs-mediated Immunosuppression through Canonical and Non-Canonical Pathways in a 4T1 Murine Breast Cancer Model
Authors: Xu Ping , Yan Feng , Zhao Yueling , Chen Xiangbo , Sun Shili , Wang Yuefei , Ying Le
Published: April 2020

Several studies in the past decades have reported anti-tumor activity of the bioactive compounds extracted from tea leaves, with a focus on the compound epigallocatechin-3-gallate (EGCG). However, further investigations are required to unravel the underlying mechanisms behind the anti-tumor activity of EGCG. In this study, we demonstrate that EGCG significantly inhibits the growth of 4T1 breast cancer cells in vitro and in vivo. EGCG ameliorated immunosuppression by significantly decreasing the accumulation of myeloid-derived suppressor cells (MDSCs) and increasing the proportions of CD4+ and CD8+ T cells in spleen and tumor sites in 4T1 breast tumor-bearing mice. Surprisingly, a low dose of EGCG (0.5–5 μg/mL) effectively reduced the cell viability and increased the apoptosis rate of MDSCs in vitro. EGCG down-regulated the canonical pathways in MDSCs, mainly through the Arg-1/iNOS/Nox2/NF-κB/STAT3 signaling pathway. Moreover, transcriptomic analysis suggested that EGCG also affected the non-canonical pathways in MDSCs, such as ECM–receptor interaction and focal adhesion. qRT-PCR further validated that EGCG restored nine key genes in MDSCs, including Cxcl3, Vcan, Col4a1, Col8a1, Oasl2, Mmp12, Met, Itsnl and Acot1. Our results provide new insight into the mechanism of EGCG-associated key pathways/genes in MDSCs in the murine breast tumor model.
Estimation of Dietary Amino Acid Intake and Independent Correlates of Skeletal Muscle Mass Index among Korean Adults
Authors: Chae Minjeong , Park Hyoungsu , Park Kyong
Published: April 2020

The aim of this study was to develop a database to identify dietary amino acid intake levels, and to determine whether any amino acid groups were independently correlated with skeletal muscle mass index (SMI). We used data from the Korea National Health and Nutrition Examination Survey 2008–2011, and a total of 3292 participants aged 50–64 years were included in the analysis. Dietary data were obtained using the 24 h recall method. Data regarding dietary amino acid intake was assessed using the computer-aided nutritional analysis program 4.0 published by the Korean Nutrition Society. Multivariate linear regression analysis was used to identify independent correlates of SMI. The major food group that contributed the highest essential amino acid intake was grain and grain products (histidine 25.5%, isoleucine 43.9%, leucine 44.2%, methionine 31.0%, phenylalanine 44.8%, tryptophan 26.4%, and valine 50.8%). Higher SMI was independently associated with sex (men), lower age and body mass index, higher levels of physical activity, and a higher intake of energy and branched-chain amino acids. These results are expected to be used as a basis for developing dietary amino acid intake guidelines for Koreans.
Intravenous Arginine Administration Benefits CD4+ T-Cell Homeostasis and Attenuates Liver Inflammation in Mice with Polymicrobial Sepsis
Authors: Yeh Chiu-Li , Tanuseputero Sharon Angela , Wu Jin-Ming , Tseng Yi-Ru , Yang Po-Jen , Lee Po-Chu , Yeh Sung-Ling , Lin Ming-Tsan
Published: April 2020

This study investigated the effects of a single dose of arginine (Arg) administration at the beginning of sepsis on CD4+ T-cell regulation and liver inflammation in C57BL/6J mice. Mice were divided into normal control (NC), sham (SH), sepsis saline (SS), and sepsis Arg (SA) groups. An inducible nitric oxide (NO) synthase (iNOS) inhibitor was administered to additional sepsis groups to evaluate the role of NO during sepsis. Sepsis was induced using cecal ligation and puncture (CLP). The SS and SA groups received saline or Arg (300 mg/kg body weight) via tail vein 1 h after CLP. Mice were euthanized at 12 and 24 h post-CLP. Blood, para-aortic lymph nodes, and liver tissues were collected for further measurement. The findings showed that sepsis resulted in decreases in blood and para-aortic lymph node CD4+ T-cell percentages, whereas percentages of interleukin (IL)-4- and IL-17-expressing CD4+ T cells were upregulated. Compared to the SS group, Arg administration resulted in maintained circulating and para-aortic lymph node CD4+ T cells, an increased Th1/Th2 ratio, and a reduced Th17/Treg ratio post-CLP. In addition, levels of plasma liver injury markers and expression of inflammatory genes in liver decreased. These results suggest that a single dose of Arg administered after CLP increased Arg availability, sustained CD4+ T-cell populations, elicited more-balanced Th1/Th2/Th17/Treg polarization in the circulation and the para-aortic lymph nodes, and attenuated liver inflammation in sepsis. The favorable effects of Arg were abrogated when an iNOS inhibitor was administered, which indicated that NO may be participated in regulating the homeostasis of Th/Treg cells and subsequent liver inflammation during sepsis.
Enteral Nutrition Supplemented with Transforming Growth Factor-β, Colostrum, Probiotics, and Other Nutritional Compounds in the Treatment of Patients with Inflammatory Bowel Disease
Authors: Triantafillidis John K. , Tzouvala Maria , Triantafyllidi Eleni
Published: April 2020

Enteral nutrition seems to play a significant role in the treatment of both adults and children with active Crohn’s disease, and to a lesser degree in the treatment of patients with active ulcerative colitis. The inclusion of some special factors in the enteral nutrition formulas might increase the rate of the efficacy. Actually, enteral nutrition enriched in Transforming Growth Factor-β reduced the activity index and maintained remission in patients with Crohn’s disease. In addition, a number of experimental animal studies have shown that colostrum exerts a significantly positive result. Probiotics of a special type and a certain dosage could also reduce the inflammatory process in patients with active ulcerative colitis. Therefore, the addition of these factors in an enteral nutrition formula might increase its effectiveness. Although the use of these formulas is not supported by large clinical trials, it could be argued that their administration in selected cases as an exclusive diet or in combination with the drugs used in patients with inflammatory bowel disease could benefit the patient. In this review, the authors provide an update on the role of enteral nutrition, supplemented with Transforming Growth Factor-β, colostrum, and probiotics in patients with inflammatory bowel disease.
Effects of Whey Protein Supplementation on Aortic Stiffness, Cerebral Blood Flow, and Cognitive Function in Community-Dwelling Older Adults: Findings from the ANCHORS A-WHEY Clinical Trial
Authors: Lefferts Wesley K. , Augustine Jacqueline A. , Spartano Nicole L. , Hughes William E. , Babcock Matthew C. , Heenan Brigid K. , Heffernan Kevin S.
Published: April 2020

ANCHORS A-WHEY was a 12-week randomized controlled trial (RCT) designed to examine the effect of whey protein on large artery stiffness, cerebrovascular responses to cognitive activity and cognitive function in older adults. Methods: 99 older adults (mean ± SD; age 67 ± 6 years, BMI 27.2 ± 4.7kg/m2, 45% female) were randomly assigned to 50g/daily of whey protein isolate (WPI) or an iso-caloric carbohydrate (CHO) control for 12 weeks (NCT01956994). Aortic stiffness was determined as carotid-femoral pulse wave velocity (cfPWV). Aortic hemodynamic load was assessed as the product of aortic systolic blood pressure and heart rate (Ao SBP × HR). Cerebrovascular response to cognitive activity was assessed as change in middle-cerebral artery (MCA) blood velocity pulsatility index (PI) during a cognitive perturbation (Stroop task). Cognitive function was assessed using a computerized neurocognitive battery. Results: cfPWV increased slightly in CHO and significantly decreased in WPI (p < 0.05). Ao SBP × HR was unaltered in CHO but decreased significantly in WPI (p < 0.05). Although emotion recognition selectively improved with WPI (p < 0.05), WPI had no effect on other domains of cognitive function or MCA PI response to cognitive activity (p > 0.05 for all). Conclusions: Compared to CHO, WPI supplementation results in favorable reductions in aortic stiffness and aortic hemodynamic load with limited effects on cognitive function and cerebrovascular function in community-dwelling older adults.
Hypoxia-Inducible Factor Inhibitors Derived from Marine Products Suppress a Murine Model of Neovascular Retinopathy
Authors: Shoda Chiho , Miwa Yukihiro , Nimura Kazumi , Okamoto Kazutoshi , Yamagami Satoru , Tsubota Kazuo , Kurihara Toshihide
Published: April 2020

Neovascular retinal degenerative diseases are the leading causes of blindness in developed countries. Anti-vascular endothelial growth factor (VEGF) therapy is commonly used to treat these diseases currently. However, recent reports indicate that long term suppression of VEGF in the eye is associated with chorioretinal atrophy. Therefore, a physiological amount of VEGF is required for retinal homeostasis. Hypoxia-inducible factor (HIF) is a transcriptional factor upstream of VEGF. We previously reported that HIF regulated pathological angiogenesis in the retina of murine models of oxygen-induced retinopathy and laser-induced choroidal neovascularization. Most of the known HIF inhibitors are anti-cancer agents which may have systemic adverse effects in for clinical use; thus, there is a need for safer and less invasive HIF inhibitors. In this study, we screened marine products, especially fish ingredients, and found that six species of fish had HIF inhibitory effects. Among them, administration of Decapterus tabl ingredients significantly suppressed retinal neovascular tufts by inhibiting HIF expression in a murine oxygen-induced retinopathy model. These results indicate that particular fish ingredients can act as anti-angiogenic agents in retinal neovascularization diseases.
That’s My Cue to Eat: A Systematic Review of the Persuasiveness of Front-of-Pack Cues on Food Packages for Children vs. Adults
Authors: Hallez Lotte , Qutteina Yara , Raedschelders Maxime , Boen Filip , Smits Tim
Published: April 2020

Packaging is increasingly recognized as an essential component of any marketing strategy. Visual and informational front-of-pack cues constitute salient elements of the environment that may influence what and how much someone eats. Considering their overwhelming presence on packaging of non-core foods, front-of-pack cues may contribute to the growing rates of overweight and obesity in children and adults. We conducted a systematic review to summarize the evidence concerning the impact of front-of-pack cues on choices and eating behaviors. Four electronic databases were searched for experimental studies (2009–present). This resulted in the inclusion of 57 studies (in 43 articles). We identified studies on children (3–12 years) and adults (≥ 18 years), but no studies on adolescents (12–18 years). The results suggest that children and adults are susceptible to packaging cues, with most evidence supporting the impact of visual cues. More specifically, children more often choose products with a licensed endorser and eat more from packages portraying the product with an exaggerated portion size. Adults’ eating behaviors are influenced by a range of other visual cues, mainly, package size and shape, and less so by informational cues such as labels.
Specific Dietary Components and Gut Microbiota Composition are Associated with Obesity in Children and Adolescents with Prader–Willi Syndrome
Authors: Garcia-Ribera Sonika , Amat-Bou Montse , Climent Eric , Llobet Marina , Chenoll Empar , Corripio Raquel , Ibáñez Lourdes , Ramon-Krauel Marta , Lerin Carles
Published: April 2020

Prader–Willi syndrome is a rare genetic disorder associated with impaired body composition, hyperphagia, and excessive weight gain. Strict dietary restrictions from an early age is crucial to prevent or delay the early onset of obesity, which is the main driver of comorbidities in these patients. The aim of this study was to identify dietary and gut microbiota components closely linked to weight status of these patients. We studied a cohort of children and adolescents with genetic diagnosis of Prader–Willi syndrome (N = 31), in which we determined adiposity by Dual-energy X-ray absorptiometry (DXA) and dietary composition with 4-day food records. Furthermore, we obtained fecal samples to assess microbiota composition by 16S sequencing. Multivariate regression models showed that body mass index standard deviation score (BMI-SDS) and body fat mass were directly associated with saturated fat intake and meat consumption, and inversely associated with fruit consumption. Furthermore, the gut microbiome from normal weight patients was characterized by higher phylogenetic diversity compared to those overweight or obese, with differential abundance of several genera, including Alistipes, Klebsiella, and Murimonas. Notably, Alistipes abundance was inversely correlated to adiposity, lipid and glucose homeostasis parameters, and meat intake. Our results suggest that limiting meat and increasing fruit intake might be beneficial for body weight management in children and adolescents with Prader–Willi syndrome.
Screening and Treatment of Vitamin D Deficiency in UK Patients with Crohn’s Disease: Self-Reported Practice among Gastroenterologists
Authors: Fletcher Jane , Swift Amelia , Hewison Martin , Cooper Sheldon C
Published: April 2020

Currently, there is no UK national recommendation to measure vitamin D levels in patients with inflammatory bowel diseases (IBD). Patients with IBD are at risk of developing vitamin D deficiency with the highest prevalence frequently reported in those with Crohn’s disease (CD). Treating vitamin D deficiency as part of CD management continues to be of interest. Our aim was to identify influences on practice and self-reported practice among British Society of Gastroenterology (BSG)-IBD section members in the screening and the treatment of vitamin D deficiency in patients with CD. A web-based survey was distributed via email to members of the BSG-IBD section. Reported screening practice was generally annual in those with a history of previous surgery related to CD or small bowel CD. A total of 83% of respondents (n = 64) thought that vitamin D levels should be routinely monitored in patients with CD. Treatments for mild/moderate deficiency included increased sunlight exposure (mean frequency = 21, SD = 15) and dietary advice (mean frequency = 22, SD = 14); in moderate/severe deficiency, oral supplementation was recommended (mean frequency = 14, SD = 13). Respondents reported factors most likely to influence practice, including clearer evidence and guidance. Well conducted studies in CD patients with identified vitamin D deficiency are needed to inform national guidance and clinical practice.
Dose-Response Relationships between Breastfeeding and Postpartum Weight Retention Differ by Pre-Pregnancy Body-Mass Index in Taiwanese Women
Authors: Waits Alexander , Guo Chao-Yu , Chang Yan-Shing , Chien Li-Yin
Published: April 2020

Postpartum weight retention (PWR) is a risk factor for future obesity. The role of breastfeeding in reducing PWR is not fully understood. We examined the relationship between PWR and the duration of exclusive/partial breastfeeding in 52,367 postpartum women from 2012–2016 Taiwan national breastfeeding surveys. The women were interviewed at 7–14 months postpartum. Non-linear models were fit to examine the association between PWR and breastfeeding duration. PWR adjusted means and 95% confidence intervals were plotted and compared for the duration of exclusive/partial breastfeeding in the total sample and between pre-pregnancy body-mass index (BMI) groups (underweight, normal, overweight, and obese). Women who breastfed exclusively for >30 days showed significantly lower PWR than those who did not breastfeed and those who breastfed partially for the same duration, thereafter each additional duration of 30 days being associated with an average of 0.1–0.2 kg less PWR. Women who breastfed partially for 120 days showed lower PWR than those who did not or those who ceased to breastfeed, thereafter each additional duration of 30 days being associated with an average of 0.1 kg less PWR. Duration of breastfeeding needed to achieve significantly less PWR differed between pre-pregnancy BMI groups, but the effect of exclusive breastfeeding appeared earlier in the normal weight group. Women with obesity who breastfed exclusively for >30 or partially for >180 days, had lower PWR than non-obese groups. The observed dose–response relationship between breastfeeding duration and PWR supports the “every feeding matters” approach in breastfeeding promotion. The larger effect of exclusive and partial breastfeeding on PWR in women with obesity may draw special attention of breastfeeding promotion.
Traditional Dietary Patterns and Risk of Mortality in a Longitudinal Cohort of the Salus in Apulia Study
Authors: Zupo Roberta , Sardone Rodolfo , Donghia Rossella , Castellana Fabio , Lampignano Luisa , Bortone Ilaria , Misciagna Giovanni , De Pergola Giovanni , Panza Francesco , Lozupone Madia , Passantino Andrea , Veronese Nicola , Guerra Vito , Boeing Heiner , Giannelli Gianluigi
Published: April 2020

There is still room for further studies analyzing the long-term health impact of specific dietary patterns observable in regions belonging to the Mediterranean area. The aim of the study is to evaluate how much a diet practiced in southern Italy is associated to a risk of mortality. The study population included 2472 participants first investigated in 1985, inquiring about their frequencies of intake of 29 foods using a self-administered questionnaire covering the previous year. The population was followed up for mortality until 31 December 2017. Cox-based risk modeling referred to single foods, food groups, the results of principal component analysis (PCA), and a priori indexes. Single food analysis revealed eggs, fatty meat, and fatty/baked ham to be inversely associated with mortality. Furthermore, one of the 5 PCA derived dietary patterns, the “Farmhouse” pattern, showed a higher hazard ratio (HR), mostly driven by dairy products. In subsequent analyses, the increased risk of mortality for fresh cheese and decreased risk for fatty ham and eggs were confirmed. The a priori diet indexes (Italian Meddiet, Meddietscore, Dietary Approaches to Stop Hypertension (DASH), and Mediterranean–DASH Intervention for Neurodegenerative Delay diet (MIND) indexes) showed borderline inverse relationships. In a Mediterranean population with an overall healthy diet, foods such as eggs and fatty meat, reflecting dietary energy and wealth, played a role in prolonging the life of individuals. Our study confirms that some dairy products might have a detrimental role in mortality in the Mediterranean setting.
Administration of Apple Polyphenol Supplements for Skin Conditions in Healthy Women: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
Authors: Shoji Toshihiko , Masumoto Saeko , Moriichi Nina , Ohtake Yasuyuki , Kanda Tomomasa
Published: April 2020

This clinical study was performed to evaluate the effects of continuous apple polyphenol (AP) administration on facial skin conditions and pigmentation induced by ultraviolet (UV) irradiation in healthy women participants. Participants (n = 65, age 20–39 years) were randomized to receive tablets containing AP (300 or 600 mg/day) or placebo in a double-blinded, placebo-controlled clinical trial. Continuous administration of AP for 12 weeks significantly prevented UV irradiation induced skin pigmentation (erythema value, melanin value, L value), although a dose-dependent relationship was not clearly observed. In contrast, no significant differences were detected between the groups with regard to water content and trans-epidermal water loss. Our study demonstrated that APs and their major active compounds, procyanidins, have several health benefits. Here, we report that continuous administration of AP for 12 weeks alleviated UV irradiation induced skin pigmentation, when compared with placebo, in healthy women.
First-degree Relatives of Celiac Disease Patients Have Increased Seroreactivity to Serum Microbial Markers
Authors: Viitasalo Liisa , Iltanen Sari , Huhtala Heini , Saavalainen Päivi , Kaukinen Katri , Lindfors Katri , Kurppa Kalle
Published: April 2020

Risk of celiac disease (CD) is increased in relatives of CD patients due to genetic and possible environmental factors. We recently reported increased seropositivity to anti-Saccharomyces cerevisiae (ASCA), Pseudomonas fluorescens-associated sequence (anti-I2) and Bacteroides caccae TonB-linked outer membrane protein (anti-OmpW) antibodies in CD. We hypothesized these markers also to be overrepresented in relatives. Seropositivity and levels of ASCA, anti-I2 and anti-OmpW were compared between 463 first-degree relatives, 58 untreated and 55 treated CD patients, and 80 controls. CD-associated human leukocyte antigen (HLA)-haplotypes and transglutaminase (tTGab) and endomysium (EmA) antibodies were determined. One or more of the microbial antibodies was present in 75% of relatives, 97% of untreated and 87% of treated CD patients and 44% of the controls. The relatives had higher median ASCA IgA (9.13 vs. 4.50 U/mL, p < 0.001), ASCA IgG (8.91 vs. 5.75 U/mL, p < 0.001) and anti-I2 (absorbance 0.74 vs. 0.32, p < 0.001) levels than controls. There was a weak, positive correlation between tTGab and ASCA (r = 0.31, p < 0.001). Seropositivity was not significantly associated with HLA. To conclude, seropositivity to microbial markers was more common and ASCA and anti-I2 levels higher in relatives of CD patients than controls. These findings were not associated with HLA, suggesting the role of other genetic and environmental factors.
Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System
Authors: Gęgotek Agnieszka , Jarocka-Karpowicz Iwona , Skrzydlewska Elżbieta
Published: April 2020

The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant and anti-inflammatory properties, can also be used to protect skin cells from the effects of UV radiation in sunlight. Here, we tested the potential protective effect of ascorbic acid and rutin used together in UVB-irradiated human skin fibroblasts, and assessed the proteomic profile of these cells, grown in a three-dimensional (3D) system. Proteomic findings revealed a combined effect of ascorbic acid and rutin in UV-irradiated fibroblasts against overexpression of pro-inflammatory signaling proteins and DNA reorganization/expression. These effects were not observed when cells were treated with either compounds alone. The antioxidant effects of ascorbic acid and rutin also prevented protein modifications by lipid peroxidation products. Further, ascorbic acid stimulated rutin-protein adduct formation, which supports intra/extracellular signaling and the Nrf2/ARE antioxidant pathway, contributing to the protective effects against UV-induced oxidative stress. The combined effect of ascorbic acid and rutin suggests that this combination of compounds is potentially effective against skin damage caused by UV radiation.
Dietary Fibre May Mitigate Sarcopenia Risk: Findings from the NU-AGE Cohort of Older European Adults
Authors: Montiel-Rojas Diego , Nilsson Andreas , Santoro Aurelia , Franceschi Claudio , Bazzocchi Alberto , Battista Giuseppe , de Groot Lisette C. P. G. M. , Feskens Edith J. M. , Berendsen Agnes , Pietruszka Barbara , Januszko Olga , Fairweather-Tait Susan , Jennings Amy , Nicoletti Claudio , Kadi Fawzi
Published: April 2020

Sarcopenia is characterised by a progressive loss of skeletal muscle mass and physical function as well as related metabolic disturbances. While fibre-rich diets can influence metabolic health outcomes, the impact on skeletal muscle mass and function is yet to be determined, and the moderating effects by physical activity (PA) need to be considered. The aim of the present study was to examine links between fibre intake, skeletal muscle mass and physical function in a cohort of older adults from the NU-AGE study. In 981 older adults (71 ± 4 years, 58% female), physical function was assessed using the short-physical performance battery test and handgrip strength. Skeletal muscle mass index (SMI) was derived using dual-energy X-ray absorptiometry (DXA). Dietary fibre intake (FI) was assessed by 7-day food record and PA was objectively determined by accelerometery. General linear models accounting for covariates including PA level, protein intake and metabolic syndrome (MetS) were used. Women above the median FI had significantly higher SMI compared to those below, which remained in fully adjusted models (24.7 ± 0.2% vs. 24.2 ± 0.1%, p = 0.011, η2p = 0.012). In men, the same association was only evident in those without MetS (above median FI: 32.4 ± 0.3% vs. below median FI: 31.3 ± 0.3%, p = 0.005, η2p = 0.035). There was no significant impact of FI on physical function outcomes. The findings from this study suggest a beneficial impact of FI on skeletal muscle mass in older adults. Importantly, this impact is independent of adherence to guidelines for protein intake and PA, which further strengthens the potential role of dietary fibre in preventing sarcopenia. Further experimental work is warranted in order to elucidate the mechanisms underpinning the action of dietary fibre on the regulation of muscle mass.
Monitoring of Pseudorabies in Wild Boar of Germany—A Spatiotemporal Analysis
Authors: Denzin Nicolai , Conraths Franz J. , Mettenleiter Thomas C. , Freuling Conrad M. , Müller Thomas
Published: April 2020

To evaluate recent developments regarding the epidemiological situation of pseudorabies virus (PRV) infections in wild boar populations in Germany, nationwide serological monitoring was conducted between 2010 and 2015. During this period, a total of 108,748 sera from wild boars were tested for the presence of PRV-specific antibodies using commercial enzyme-linked immunosorbent assays. The overall PRV seroprevalence was estimated at 12.09% for Germany. A significant increase in seroprevalence was observed in recent years indicating both a further spatial spread and strong disease dynamics. For spatiotemporal analysis, data from 1985 to 2009 from previous studies were incorporated. The analysis revealed that PRV infections in wild boar were endemic in all German federal states; the affected area covers at least 48.5% of the German territory. There were marked differences in seroprevalence at district levels as well as in the relative risk (RR) of infection of wild boar throughout Germany. We identified several smaller clusters and one large region, where the RR was two to four times higher as compared to the remaining areas under investigation. Based on the present monitoring intensity and outcome, we provide recommendations with respect to future monitoring efforts concerning PRV infections in wild boar in Germany.
COVID-19: A Brief Overview of the Discovery Clinical Trial
Authors: Vanden Eynde Jean Jacques
Published: April 2020

The outbreak of COVID-19 is leading to a tremendous search for curative treatments. The urgency of the situation favors a repurposing of active drugs but not only antivirals. This short communication focuses on four treatments recommended by WHO and included in the first clinical trial of the European Discovery project.
Generation of over 1000 Diffraction Spots from 2D Graded Photonic Super-Crystals
Authors: Hassan Safaa , Jiang Yan , Alnasser Khadijah , Hurley Noah , Zhang Hualiang , Philipose Usha , Lin Yuankun
Published: April 2020

For the first time, we are able to generate over 1000 diffraction spots from a graded photonic super-crystal with a unit super-cell size of 12a × 12a where a is the lattice constant and hole radii are gradually changed in dual directions. The diffraction pattern from the graded photonic super-crystal reveals unique diffraction properties. The first order diffractions of (±1,0) or (0,±1) disappear. Fractional diffraction orders are observed in the diffraction pattern inside a square with vertices of (1,1), (1,−1), (−1,−1) and (−1,−1). The fractional diffraction can be understood from lattices with a period of a. However, a dual-lattice model is considered in order to explain higher-order diffractions. E-field intensity simulations show a coupling and re-distribution among fractional orders of Bloch waves. There are a total of 12 × 12 spots in E-field intensity in the unit supercell corresponding to 12 × 12 fractional diffraction orders in the diffraction pattern and 12 × 12 fractional orders of momentum in the first Brillouin zone in k-space.
History and Some Aspects of the Lamb Shift
Authors: Maclay G. Jordan
Published: April 2020

Radiation is a process common to classical and quantum systems with very different effects in each regime. In a quantum system, the interaction of a bound electron with its own radiation field leads to complex shifts in the energy levels of the electron, with the real part of the shift corresponding to a shift in the energy level and the imaginary part to the width of the energy level. The most celebrated radiative shift is the Lamb shift between the 2 s 1 / 2 and the 2 p 1 / 2 levels of the hydrogen atom. The measurement of this shift in 1947 by Willis Lamb Jr. proved that the prediction by Dirac theory that the energy levels were degenerate was incorrect. Hans Bethe’s calculation of the shift showed how to deal with the divergences plaguing the existing theories and led to the understanding that interactions with the zero-point vacuum field, the lowest energy state of the quantized electromagnetic field, have measurable effects, not just resetting the zero of energy. This understanding led to the development of modern quantum electrodynamics (QED). This historical pedagogic paper explores the history of Bethe’s calculation and its significance. It explores radiative effects in classical and quantum systems from different perspectives, with the emphasis on understanding the fundamental physical phenomena. Illustrations are drawn from systems with central forces, the H atom, and the three-dimensional harmonic oscillator. A first-order QED calculation of the complex radiative shift for a spinless electron is explored using the equations of motion and the m a s s 2 operator, describing the fundamental phenomena involved, and relating the results to Feynman diagrams.
Estimating Stochastic Volatility under the Assumption of Stochastic Volatility of Volatility
Authors: Alghalith Moawia , Floros Christos , Gkillas Konstantinos
Published: April 2020

We propose novel nonparametric estimators for stochastic volatility and the volatility of volatility. In doing so, we relax the assumption of a constant volatility of volatility and therefore, we allow the volatility of volatility to vary over time. Our methods are exceedingly simple and far simpler than the existing ones. Using intraday prices for the Standard & Poor’s 500 equity index, the estimates revealed strong evidence that both volatility and the volatility of volatility are stochastic. We also proceeded in a Monte Carlo simulation analysis and found that the estimates were reasonably accurate. Such evidence implies that the stochastic volatility models proposed in the literature with constant volatility of volatility may fail to approximate the discrete-time short rate dynamics.
Combining Radar and Optical Imagery to Map Oil Palm Plantations in Sumatra, Indonesia, Using the Google Earth Engine
Authors: Sarzynski Thuan , Giam Xingli , Carrasco Luis , Lee Janice Ser Huay
Published: April 2020

Monitoring the expansion of commodity crops in the tropics is crucial to safeguard forests for biodiversity and ecosystem services. Oil palm (Elaeis guineensis) is one such crop that is a major driver of deforestation in Southeast Asia. We evaluated the use of a semi-automated approach with random forest as a classifier and combined optical and radar datasets to classify oil palm land-cover in 2015 in Sumatra, Indonesia, using Google Earth Engine. We compared our map with two existing remotely-sensed oil palm land-cover products that utilized visual and semi-automated approaches for the same year. We evaluated the accuracy of oil palm land-cover classification from optical (Landsat), radar (synthetic aperture radar (SAR)), and combined optical and radar satellite imagery (Combined). Combining Landsat and SAR data resulted in the highest overall classification accuracy (84%) and highest producer’s and user’s accuracy for oil palm classification (84% and 90%, respectively). The amount of oil palm land-cover in our Combined map was closer to official government statistics than the two existing land-cover products that used visual interpretation techniques. Our analysis of the extents of disagreement in oil palm land-cover indicated that our map had comparable accuracy to one of them and higher accuracy than the other. Our results demonstrate that a combination of optical and radar data outperforms the use of optical-only or radar-only datasets for oil palm classification and that our technique of preprocessing and classifying combined optical and radar data in the Google Earth Engine can be applied to accurately monitor oil-palm land-cover in Southeast Asia.
Azimuth Ambiguity Suppression for Hybrid Polarimetric Synthetic Aperture Radar via Waveform Diversity
Authors: Zhao Pengfei , Deng Yunkai , Wang Wei , Liu Dacheng , Wang Robert
Published: April 2020

Hybrid quadrature polarimetric (hybrid quad-pol) synthetic aperture radar (SAR) is proposed as a potential candidate for the full-polarimetric SAR mode. It allows balanced range ambiguity performance and simplified system structure. System based on hybrid-pol SAR mode can also implement the conventional quad-pol mode and the compact-pol mode via few adjustments. However, the azimuth ambiguity performance in cross-pol channels is proved deteriorated in hybrid quad-pol mode due to the lopsided energy distribution of ambiguities. As are generally called “ghost” targets, azimuth ambiguities usually influence the recognition of the targets in SAR imaging. This letter describes how to remove the false targets that arise from azimuth ambiguities by means of waveform diversity and dual-focus post-processing (DFPP) technique. The proposed method exploits the feature of azimuth ambiguity and yields improved image quality in cross-pol channels with strong co-pol azimuth ambiguities removed in hybrid quad-pol SAR at a low system cost. Furthermore, it offers remarkable benefits for target detecting and recognition with strong false targets removed.
Application of Kinematic GPR-TPS Model with High 3D Georeference Accuracy for Underground Utility Infrastructure Mapping: A Case Study from Urban Sites in Celje, Slovenia
Authors: Šarlah Nikolaj , Podobnikar Tomaž , Ambrožič Tomaž , Mušič Branko
Published: April 2020

This paper describes in detail the applicability of the developed ground-penetrating radar (GPR) model with a kinematic GPR and self-tracking (robotic) terrestrial positioning system (TPS) surveying setup (GPR-TPS model) for the acquisition, processing and visualisation of underground utility infrastructure (UUI) in a real urban environment. The integration of GPR with TPS can significantly improve the accuracy of UUI positioning in a real urban environment by means of efficient control of GPR trajectories. Two areas in the urban part of Celje in Slovenia were chosen. The accuracy of the kinematic GPR-TPS model was analysed by comparing the three-dimensional (3D) position of UUI given as reference values (true 3D position) from the officially consolidated cadastre of utility infrastructure in the Republic of Slovenia and those obtained by the GPR-TPS method. To determine the reference 3D position of the GPR antenna and UUI, the same positional and height geodetic network was used. Small unmanned aerial vehicles (UAV) were used for recording to provide a better spatial display of the results of UUI obtained with the GPR-TPS method. As demonstrated by the results, the kinematic GPR-TPS model for data acquisition can achieve an accuracy of fewer than 15 centimetres in a real urban environment.