bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between Activity Pacing, Fatigue, and Physical Activity in Adults with Multiple Sclerosis: A Cross Sectional Study
Authors: Abonie Ulric S. , Hoekstra Femke , Seves Bregje L. , Woude Lucas H. V. van der , Dekker Rienk , Hettinga Florentina J.
Published: June 2020

Fatigue is common in people with multiple sclerosis (MS). Activity pacing is a behavioral way to cope with fatigue and limited energy resources. However, little is known about how people with MS naturally pace activities to manage their fatigue and optimize daily activities. This study explored how activity pacing relates to fatigue and physical activity in people with MS. Participants were 80 individuals (60 females, 20 males) with a diagnosis of MS. The participants filled in questionnaires on their activity pacing, fatigue, physical activity, and health-related quality of life, 3–6 weeks before discharge from rehabilitation. The relationships between the variables were examined using hierarchical regression. After controlling for demographics, health-related quality of life, and perceived risk of overactivity, no associations were found between activity pacing and fatigue (β = 0.20; t = 1.43, p = 0.16) or between activity pacing and physical activity (β = −0.24; t = −1.61, p = 0.12). The lack of significant associations between activity pacing and fatigue or physical activity suggests that without interventions, there appears to be no clear strategy amongst people with MS to manage fatigue and improve physical activity. People with MS may benefit from interventions to manage fatigue and optimize engagement in physical activity.
In Vitro Study of Shear Bond Strength in Direct and Indirect Bonding with Three Types of Adhesive Systems
Authors: Iglesias Angelica , Flores Teresa , Moyano Javier , Artés Montserrat , Gil Francisco Javier , Puigdollers Andreu
Published: June 2020

This study aimed to compare the shear bond strength (SBS) and adhesive remaining index (ARI) using one conventional and two novel adhesive systems with clinical step reduction and direct and indirect bonding. A sample of 72 human premolars were divided into six groups of 12 samples. The first three groups (G1, G2, G3) were bonded with a direct technique, while the remaining groups (G4, G5, G6) were bonded by the indirect technique. Groups G1 and G4 used conventional acid-etching primer composite (XT); groups G2 and G5 used self-etching bonding (BO), and groups G3 and G6 had an acid-etching treatment followed by a self-adhesive composite (OC). All groups were exposed to thermocycling. Shear bond strength was analyzed with a universal test machine, and the ARI was examined with 4× magnification. The results showed statistically significant differences between the three adhesive systems. The highest strength values were observed in the XT group G1 (13.54 ± 4 MPa), while the lowest were shown in the BO G2 samples (5.05 ± 2 MPa). There was no significant difference between the direct or indirect bonding techniques on the three compared groups. The type of primer and bonding material significantly influenced the SBS. Values with self-etching bonding were below the minimum recommended for clinical use (5.9–7.8 MPa). There was no difference between indirect and direct bonding techniques. The lowest ARI scores (0–1) were observed in both self-etching and BO groups. Further clinical studies are needed to compare in vivo results.
Modulated Photocurrent Spectroscopy Study of the Electronic Transport Properties of Working Organic Photovoltaics: Degradation Analysis
Authors: Nakatsuka Emi , Kumoda Yo , Mori Kiyohito , Kobayashi Takashi , Nagase Takashi , Naito Hiroyoshi
Published: June 2020

Electronic transport measurement using modulated photocurrent (MPC) spectroscopy is demonstrated herein in working organic photovoltaics (OPVs) before and after AM1.5G irradiation. OPVs with bulk heterojunction (BHJ) using prototypical donor and acceptor materials, poly[[4,8-bis[(2-ethylhexyl)oxy]benzo[1–2-b:4,5-b′]dithiophene-2,6-diyl][3-fluoro-2-[(2-ethylhexyl)carbonyl] = hieno [3–4-b]thiophenediyl]] (PTB7) and [6,6]-phenyl-C71-butyric acid methyl ester (PC71BM), were fabricated. The OPVs had inverted structures (BHJs are formed on transparent conductive oxide substrates). The photovoltaic performance of PTB7:PC71BM OPVs was characterized and the best power conversion efficiency was obtained at PTB7 content of 40 wt%. Electron and hole mobility were determined with MPC spectroscopy in PTB7:PC71BM OPVs and were well balanced at PTB7 content of 40 wt%. Degradation of the photovoltaic performance of PTB7:PC71BM OPVs with PTB7 content of 40 wt% caused by AM1.5G irradiation was studied. MPC spectroscopy showed that the well-balanced mobility was not affected by AM1.5G irradiation. The degradation of OPVs was not due to changes in the electronic transport properties, but mainly to the reduced short circuit current (Jsc) and fill factor (FF). The origin of this reduction is discussed.
Nugget Formation and Mechanical Behaviour of Friction Stir Welds of Three Dissimilar Aluminum Alloys
Authors: Manuel Neves , Galvão Ivan , Leal Rui M. , Costa José D. , Loureiro Altino
Published: June 2020

The aim of this research was to investigate the influence of the properties of the base materials and welding speed on the morphology and mechanical behavior of the friction stir welds of three dissimilar aluminum alloys in a T-joint configuration. The base materials were the AA2017-T4, AA5083-H111, and AA6082-T6 alloys in 3 mm-thick sheets. The AA6082-T6 alloy was the stringer, and the other alloys were located either on the advancing or retreating sides of the skin. All the T-joint welds were produced with a constant tool rotation speed but with different welding speeds. The microstructures of the welds were analyzed using optical microscopy, scanning electron microscopy with energy dispersive spectroscopy, and the electron backscatter diffraction technique. The mechanical properties were assessed according to micro-hardness, tensile, and fatigue testing. Good quality welds of the three dissimilar aluminum alloys could be achieved with friction stir welding, but a high ratio between the tool’s rotational and traverse speeds was required. The welding speed influenced the weld morphology and fatigue strength. The positioning of the skin materials influenced the nugget morphology and the mechanical behavior of the joints. The joints in which the AA2017 alloy was positioned on the advancing side presented the best tensile properties and fatigue strength.
Numerical Simulation Development and Computational Optimization for Directed Energy Deposition Additive Manufacturing Process
Authors: Kiran Abhilash , Hodek Josef , Vavřík Jaroslav , Urbánek Miroslav , Džugan Jan
Published: June 2020

The rapid growth of Additive Manufacturing (AM) in the past decade has demonstrated a significant potential in cost-effective production with a superior quality product. A numerical simulation is a steep way to learn and improve the product quality, life cycle, and production cost. To cope with the growing AM field, researchers are exploring different techniques, methods, models to simulate the AM process efficiently. The goal is to develop a thermo-mechanical weld model for the Directed Energy Deposition (DED) process for 316L stainless steel at an efficient computational cost targeting to model large AM parts in residual stress calculation. To adapt the weld model to the DED simulation, single and multi-track thermal simulations were carried out. Numerical results were validated by the DED experiment. A good agreement was found between predicted temperature trends for numerical simulation and experimental results. A large number of weld tracks in the 3D solid AM parts make the finite element process simulation challenging in terms of computational time and large amounts of data management. The method of activating elements layer by layer and introducing heat in a cyclic manner called a thermal cycle heat input was applied. Thermal cycle heat input reduces the computational time considerably. The numerical results were compared to the experimental data for thermal and residual stress analyses. A lumping of layers strategy was implemented to reduce further computational time. The different number of lumping layers was analyzed to define the limit of lumping to retain accuracy in the residual stress calculation. The lumped layers residual stress calculation was validated by the contour cut method in the deposited sample. Thermal behavior and residual stress prediction for the different numbers of a lumped layer were examined and reported computational time reduction.
Three-Dimensional (3D) Conductive Network of CNT-Modified Short Jute Fiber-Reinforced Natural Rubber: Hierarchical CNT-Enabled Thermoelectric and Electrically Conductive Composite Interfaces
Authors: Tzounis Lazaros , Petousis Markos , Liebscher Marco , Grammatikos Sotirios , Vidakis Nectarios
Published: June 2020

Jute fibers (JFs) coated with multiwall carbon nanotubes (MWCNTs) have been introduced in a natural rubber (NR) matrix creating a three-dimensional (3D) electrically conductive percolated network. The JF-CNT endowed electrical conductivity and thermoelectric properties to the final composites. CNT networks fully covered the fiber surfaces as shown by the corresponding scanning electron microscopy (SEM) analysis. NR/JF-CNT composites, at 10, 20 and 30 phr (parts per hundred gram of rubber) have been manufactured using a two-roll mixing process. The highest value of electrical conductivity (σ) was 81 S/m for the 30 phr composite. Thermoelectric measurements revealed slight differences in the Seebeck coefficient (S), while the highest power factor (PF) was 1.80 × 10−2 μW/m K−2 for the 30 phr loading. The micromechanical properties and electrical response of the composite’s conductive interface have been studied in peak force tapping quantitative nanomechanical (PFT QNM) and conductive atomic force microscopy (c-AFM) mode. The JF-CNT create an electrically percolated network at all fiber loadings endowing electrical and thermoelectric properties to the NR matrix, considered thus as promising thermoelectric stretchable materials.
Bacterial Surface Colonization of Sputter-Coated Platinum Films
Authors: Czerwińska-Główka Dominika , Przystaś Wioletta , Zabłocka-Godlewska Ewa , Student Sebastian , Cwalina Beata , Łapkowski Mieczysław , Krukiewicz Katarzyna
Published: June 2020

Due to its biocompatibility and advantageous electrochemical properties, platinum is commonly used in the design of biomedical devices, e.g., surgical instruments, as well as electro-medical or orthopedic implants. This article verifies the hypothesis that a thin layer of sputter-coated platinum may possess antibacterial effects. The purpose of this research was to investigate the adhesion and growth ability of a model strain of Gram-negative bacteria, Escherichia coli, on a surface of a platinum-coated glass slide. Although some previous literature reports suggests that a thin layer of platinum would inhibit the formation of bacterial biofilm, the results of this study suggest otherwise. The decrease in the number of bacterial cells attached to the platinum-coated glass, which was observed within first three hours of culturing, was found to be a short-time effect, vanishing after 24 h. Consequently, it was shown that a thin layer of sputter-coated platinum did not exhibit any antibacterial effect. For this reason, this study indicates an urgent need for the development of new methods of surface modification that could reduce bacterial surface colonization of platinum-based biomedical devices.
Effects of Steelmaking Slag and Moisture on Electrical Properties of Concrete
Authors: Hong Se-Hee , Yuan Tian-Feng , Choi Jin-Seok , Yoon Young-Soo
Published: June 2020

Strain sensors can indicate the conditions of concrete structures, but these sensors are only capable of measuring local behaviors of materials. To solve this problem, researchers have introduced conductive materials that can monitor the overall behavior of concrete structures. Steelmaking slag, which contains large amounts of iron oxide (Fe2O3), is conductive, and researchers have considered the addition of this material to improve concrete monitoring. In this study, mechanical and electrical properties of concrete containing steelmaking slag as a binder were evaluated. As the incorporation of steelmaking slag increased, the setting times were delayed, but the compressive strengths were similar within the replacement ratio of 15%. It was found that the addition of steelmaking slag with Fe2O3, the main ingredient of magnetite (Fe3O4), improved the electrical resistivity, piezoresistivity, and sensitivity of the concrete. Drying of the concretes resulted in an increase in electrical resistance and fractional change in resistivity (FCR). Expansion of steelmaking slag, due to contacting of free CaO and moisture under repeated loads, resulted in cracks in the concrete and affected the gauge factor (GF). This study demonstrates the possibility that the addition of steelmaking slag as a binder may provide an economical and environmentally-friendly solution to concrete strain monitoring.
Out-Of-Plane Permeability Evaluation of Carbon Fiber Preforms by Ultrasonic Wave Propagation
Authors: Lionetto Francesca , Montagna Francesco , Maffezzoli Alfonso
Published: June 2020

Out-of-plane permeability of reinforcement preforms is of crucial importance in the infusion of large and thick composite panels, but so far, there are no standard experimental methods for its determination. In this work, an experimental set-up for the measurement of unsaturated through thickness permeability based on the ultrasonic wave propagation in pulse echo mode is presented. A single ultrasonic transducer, working both as emitter and receiver of ultrasonic waves, was used to monitor the through thickness flow front during a vacuum assisted resin infusion experiment. The set-up was tested on three thick carbon fiber preforms, obtained by stacking thermal bonding of balanced or unidirectional plies either by automated fiber placement either by hand lay-up of unidirectional plies. The ultrasonic data were used to calculate unsaturated out-of-plane permeability using Darcy’s law. The permeability results were compared with saturated out-of-plane permeability, determined by a traditional gravimetric method, and validated by some analytical models. The results demonstrated the feasibility and potential of the proposed set-up for permeability measurements thanks to its noninvasive character and the one-side access.
Influence of Impact Velocity on the Residual Stress, Tensile Strength, and Structural Properties of an Explosively Welded Composite Plate
Authors: Karolczuk Aleksander , Kluger Krzysztof , Derda Szymon , Prażmowski Mariusz , Paul Henryk
Published: June 2020

This study aimed to analyze the effect of the impact velocity of a Zr 700 flyer plate explosively welded to a Ti Gr. 1/P265GH bimetallic composite on the residual stress formation, structural properties, and tensile strength. The residual stresses were determined by the orbital hole-drilling strain-gauge method in a surface layer of Zr 700 in as-received and as-welded conditions. The analysis of the tensile test results based on a force parallel to interfaces was used to propose a model for predicting the yield force of composite plates. Compressive residual stresses found in the initial state of the Zr 700 plate were transformed to tensile stresses on the surface layer of the welded Zr 700 plate. A higher impact velocity resulted in higher tensile stresses in the Zr 700 surface layer. To increase the resistance of the composite plate to stress-based corrosion cracking, a lower value of impact velocity is recommended in the welding process.
Wear Morphology on the Surfaces of CoCrMo Unicompartmental Knee Joint Endoprostheses as Elements of Metal–Metal Friction Nodes
Authors: Szarek Arkadiusz , Stradomski Grzegorz , Łukomska-Szarek Justyna , Rydz Dariusz , Wolański Wojciech , Joszko Kamil
Published: June 2020

The article assesses the strength and structural parameters of load-bearing layers of metal biobearings made of CoCrMo alloy. The research material consisted of unicompartmental knee joint endoprostheses used in the human body, removed due to excessive wear. No patient participated in the examinations. The endoprostheses used as research material underwent the liquidation procedures in the hospital, which has all necessary permissions and certifications to perform endoprosthetic procedures. Endoprostheses selected for the examinations had been used for 6 to 12 years at similar load conditions as declared by the patients, i.e., body weight of F = 835 N, declared activity expressed as the number of load cycles up to 100 thousand/year, and no artificial joint infections. To assess the homogeneity of the research material, the analysis of chemical composition using a Joel scanning electron microscope with EDS (Energy-dispersive X-ray spectroscopy) was made to exclude endoprostheses with various alloying additives. Microscopic examinations were performed using the Phenom XL microscope, while the wear surface was examined using a Keyence VHX-900F microscope. Several experimental tests were also carried out on load-bearing surfaces to assess changes in strength parameters of the base material after a known life cycle and load conditions. Material hardness using the Vickers method, yield point, critical value of stress intensity coefficient, and the coefficient of friction µ were evaluated. The examinations allowed for the systematization of wear in the knee and femoral components of unicompartmental hip endoprostheses. The statistical evaluation of the number and costs of hip joint replacement surgeries in Poland was also made.
Toluene Adsorption by Mesoporous Silicas with Different Textural Properties: A Model Study for VOCs Retention and Water Remediation
Authors: Vittoni Chiara , Gatti Giorgio , Braschi Ilaria , Buscaroli Enrico , Golemme Giovanni , Marchese Leonardo , Bisio Chiara
Published: June 2020

In this work, different mesoporous silicas were studied as potential sorbents for toluene, selected as a model molecule of aromatic organic fuel-based pollutants. Three siliceous materials with different textural and surface properties (i.e., fumed silica and mesoporous Santa Barbara Amorphous (SBA)-15 and Mobil Composition of matter (MCM)-41 materials) were considered and the effect of their physico-chemical properties on the toluene adsorption process was studied. In particular, FT-IR spectroscopy was used to qualitatively study the interactions between the toluene molecule and the surface of silicas, while volumetric adsorption analysis allowed the quantitative determination of the toluene adsorption capacity. The combined use of these techniques revealed that textural properties of the sorbents, primarily porosity, are the driving forces that control the adsorption process. Considering that, under real conditions of usage, the sorbents are soaked in water, their hydrothermal stability was also investigated and toluene adsorption by both the gas and aqueous phase on hydrothermally pre-treated samples was studied. The presence of ordered porosity, together with the different pore size distribution and the amount of silanol groups, strongly affected the adsorption process. In toluene adsorption from water, SBA-15 performed better than MCM-41.
Melt Conditioned Direct Chill (MC-DC) Casting and Extrusion of AA5754 Aluminium Alloy Formulated from Recycled Taint Tabor Scrap
Authors: Al-Helal Kawther , Patel Jayesh B. , Scamans Geoff M. , Fan Zhongyun
Published: June 2020

The melt conditioned direct chill (MC-DC) casting process has been used to produce billets and extruded planks of AA5754 alloy formulated from 100% recycled Taint Tabor scrap aluminum. The billets were homogenized and then extruded into flat planks. Optical metallography of the MC-DC cast billets showed equiaxed refined grains in comparison to conventional direct chill (DC) cast and direct chill grain refined (DC-GR) cast billets formulated from the same Taint Tabor scrap. Microstructural evaluation of the extruded planks showed extensive peripheral coarse grain (PCG) for the DC, DC-GR and MC-DC cast planks. The 2 mm and 1 mm MC-DC cast planks produced after cold rolling and heat treatment showed a fully recrystallized microstructure at 380 °C and 300 °C for 10 min respectively with an improvement in mechanical properties over DC-GR cast and similarly processed planks. The as-extruded MC-DC cast planks tensile tested in the transverse direction showed 34% elongation and 213 MPa ultimate tensile strength. These tensile results showed 5.8% higher elongation and 1.2% higher ultimate tensile strength compared with the DC-GR planks after applying high shear melt conditioning.
Enhanced Erosion–Corrosion Resistance of Tungsten by Carburizing Using Spark Plasma Sintering Technique
Authors: Jiang Yan , Yang Junfeng , Xie Zhuoming , Fang Qianfeng
Published: June 2020

The biggest obstacle for the application of tungsten as the target materials in the spallation neutron source is its serious corrosion in the coolant of flowing water. For this reason, W–Cr–C clad tungsten was developed by tungsten carburizing in a spark plasma sintering device, with superior corrosion resistance in the static immersion and electrochemical corrosion test. This work focused on its erosion and corrosion performance in a flowing water system, based upon test parameters simulated under the service conditions. W–Cr–C clad tungsten showed superior corrosion resistance to that of bare tungsten due to the corrosion form changing from the intergranular corrosion of bare tungsten to pitting corrosion of W–Cr–C coating. The corrosion rate of tungsten was as high as tenfold that of the coated sample at 20 °C, and at most fourfold at 60 °C after testing for 360 h. Effects of water velocity and temperature on pitting and intergranular corrosion were investigated in detail and their corresponding corrosion mechanisms were analyzed and discussed.
Effect of Low-Frequency Electromagnetic Casting on Micro-Structure and Macro-Segregation of 5A90 Alloy Ingots
Authors: Wang Fuyue , Wang Xiangjie , Cui Jianzhong
Published: June 2020

The effect of low-frequency electromagnetic fields on the micro-structure and macro-segregation of 5A90 alloy ingots during the semi-continuous casting process were quantitatively investigated. The ingots of a 5A90 alloy with a diameter 170 mm were produced by the conventional direct chill casting (DCC) process and low-frequency electromagnetic casting (LFEC) with 10 Hz/100 A. The results showed that LFEC can substantially refine the micro-structure and shorten the width of the columnar grain area of an ingot. The refinement effect came with the relieving of grain boundary segregation and an improvement in the macro-segregation of the ingot. Compared with the traditional DCC process, the tensile properties of the aged alloy prepared by the LFEC process were improved due to the effects of the increase in solid solubility and the strengthening of the grain refinement, so that the stability of the tensile properties was also improved. Meanwhile, the rate of yield increased by 2.3% with a decrease in the peeling thickness of the ingot.
Applying Fixed Point Techniques to Stability Problems in Intuitionistic Fuzzy Banach Spaces
Authors: Saha P. , Samanta T. K. , Mondal Pratap , Choudhury B. S. , De La Sen Manuel
Published: June 2020

In this paper we investigate Hyers-Ulam-Rassias stability of certain nonlinear functional equations. Considerations of such stabilities in different branches of mathematics have been very extensive. Again the fuzzy concepts along with their several extensions have appeared in almost all branches of mathematics. Here we work on intuitionistic fuzzy real Banach spaces, which is obtained by combining together the concepts of fuzzy Banach spaces with intuitionistic fuzzy sets. We establish that pexiderized quadratic functional equations defined on such spaces are stable in the sense of Hyers-Ulam-Rassias stability. We adopt a fixed point approach to the problem. Precisely, we use a generxalized contraction mapping principle. The result is illustrated with an example.
Exploring the Microalga Euglena cantabrica by Pressurized Liquid Extraction to Obtain Bioactive Compounds
Authors: Muñóz-Almagro Nerea , Gilbert-López Bienvenida , M. Carmen Pozuelo-Rollón , García-Fernandez Yolanda , Almeida Carlos , Villamiel Mar , Mendiola Jose A. , Ibáñez Elena
Published: June 2020

In the present study, the chemical composition of the microalga Euglena cantabrica was investigated. The extraction of bioactive compounds was done using pressurized liquid extraction (PLE) at different temperatures (40–180 °C) and using green solvents (ethanol-water mixtures). A statistical design of experiments was used to optimize the maximum antioxidant capacity of the extracts by response surface methodology. The antioxidant capacity was determined through the inhibition of 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, while the chemical analyses of the extracts were carried out using different chromatographic techniques. Chlorophylls and carotenoids were analyzed by high-performance liquid chromatography coupled to a diode array detector and mass spectrometry (HPLC-DAD-MS/MS) and carbohydrates by gas chromatography with flame ionization detection (GC-FID) and high-pressure size-exclusion chromatography coupled to an evaporative light-scattering detector (HPSEC-ELSD). The results showed different possibilities for the extraction conditions, depending on the desired bioactivity or chemical composition. Briefly, (i) mixtures of ethanol-water containing around 40% ethanol at 180 °C gave the best antioxidant capacity, (ii) mixtures containing around 50% ethanol at 110 °C gave the best yield of β-glucan paramylon, and (iii) the use of pure ethanol at a low temperature (40 °C) is the best choice for the recovery of carotenoids such as diatoxanthin. Summing up, E. cantabrica seems to be a good candidate to be used in biorefinery to obtain different bioactive compounds.
Mussel Shell-Derived Macroporous 3D Scaffold: Characterization and Optimization Study of a Bioceramic from the Circular Economy
Authors: Scialla Stefania , Carella Francesca , Dapporto Massimiliano , Sprio Simone , Piancastelli Andreana , Palazzo Barbara , Adamiano Alessio , Degli Esposti Lorenzo , Iafisco Michele , Piccirillo Clara
Published: June 2020

Fish industry by-products constitute an interesting platform for the extraction and recovery of valuable compounds in a circular economy approach. Among them, mussel shells could provide a calcium-rich source for the synthesis of hydroxyapatite (HA) bioceramics. In this work, HA nanoparticles have been successfully synthesized starting from mussel shells (Mytilus edulis) with a two steps process based on thermal treatment to convert CaCO3 in CaO and subsequent wet precipitation with a phosphorus source. Several parameters were studied, such as the temperature and gaseous atmosphere of the thermal treatment as well as the use of two different phosphorus-containing reagents in the wet precipitation. Data have revealed that the characteristics of the powders can be tailored, changing the conditions of the process. In particular, the use of (NH4)2HPO4 as the phosphorus source led to HA nanoparticles with a high crystallinity degree, while smaller nanoparticles with a higher surface area were obtained when H3PO4 was employed. Further, a selected HA sample was synthesized at the pilot scale; then, it was employed to fabricate porous 3D scaffolds using the direct foaming method. A highly porous scaffold with open and interconnected porosity associated with good mechanical properties (i.e., porosity in the range 87–89%, pore size in the range 50–300 μm, and a compressive strength σ = 0.51 ± 0.14 MPa) suitable for bone replacement was achieved. These results suggest that mussel shell by-products are effectively usable for the development of compounds of high added value in the biomedical field.
Bioactive Molecular Networking for Mapping the Antimicrobial Constituents of the Baltic Brown Alga Fucus vesiculosus
Authors: Buedenbender Larissa , Astone Francesca Anna , Tasdemir Deniz
Published: June 2020

The brown alga Fucus vesiculosus is common to the intertidal zones of the Baltic Sea, where it is exposed to high fouling pressures by microorganisms. Our previous studies showed, repeatedly, the consistent antimicrobial activity of F. vesiculosus crude extracts against human pathogens, while untargeted metabolomics analyses have revealed a variety of metabolites. In this study, we applied the UPLC-QToF-MS/MS-based “bioactive molecular networking” (BMN) concept on the most bioactive n-hexane and n-butanol subextracts of Baltic F. vesiculosus coupled with in silico dereplication tools to identify the compounds responsible for antimicrobial activity. The first antimicrobial cluster identified by BMN was galactolipids. Our targeted isolation efforts for this class led to the isolation of six monogalactosyldiacylglycerol (MGDG) derivatives (1–6) and one digalactosyldiacylglycerol (DGDG, 7). The MGDGs 5 and 6 and the DGDG 7 exhibited activity against Staphylococcus aureus. The second compound class with high bioactivity was phlorotannins. In particular, phlorethol-type phlorotannins showed high correlations with antimicrobial activity based on the BMN approach, and two phlorotannins (8–9) were isolated. This study shows that antimicrobial components of F. vesiculosus reside in the algal cell walls and membranes and that BMN provides a complementary tool for the targeted isolation of bioactive metabolites.
Protective Effect of a Fucose-Rich Fucoidan Isolated from Saccharina japonica against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish
Authors: Su Wanchun , Wang Lei , Fu Xiaoting , Ni Liying , Duan Delin , Xu Jiachao , Gao Xin
Published: June 2020

A fucose-rich fucoidan was purified from brown seaweed Saccharina japonica, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry.
Dental Care Provision during Coronavirus Disease 2019 (COVID-19) Pandemic: The Importance of Continuous Support for Vulnerable Patients
Authors: Dziedzic Arkadiusz , Tanasiewicz Marta , Tysiąc-Miśta Monika
Published: June 2020

As a result of the ongoing 2019 coronavirus disease (COVID-19) pandemic, the medical and dental services across the world have to tackle unprecedented situations, providing essential care and professional support. The global health care crisis caused directly by the vast number of severe COVID-19 cases, and indirectly by reduced access to health care, as well as by limited secondary care provision, had a major impact on specialist services, and subsequently the deterioration of medical and dental conditions, particularly in vulnerable persons. In particular, at present, special care dentistry seems to play a unique role, dealing with a wide range of patients with underlying medical conditions and co-morbidities, phobic individuals, and persons with learning/physical disabilities. The effective adaptation of health services to the current new reality, based on an empathetic approach and recent guidelines, would allow us to maintain an adequate care provision, minimizing the long-term impact of the pandemic.
Oxidative Stress and Antioxidant Responses of Phormidium ambiguum and Microcystis aeruginosa Under Diurnally Varying Light Conditions
Authors: Muhetaer Guligena , Jayasanka Senavirathna M.D.H. , Fujino Takeshi
Published: June 2020

Two harmful cyanobacteria species (Phormidium ambiguum and Microcystis aeruginosa) were exposed to diurnal light-intensity variation to investigate their favorable and stressed phases during a single day. The photosynthetically active radiation (PAR) started at 0 µmol·m−2·s−1 (06:00 h), increased by ~25 µmol·m−2·s−1 or ~50 µmol·m−2·s−1 every 30 min, peaking at 300 µmol·m−2·s−1 or 600 µmol·m−2·s−1 (12:00 h), and then decreased to 0 µmol·m−2·s−1 (by 18:00 h). The H2O2 and antioxidant activities were paralleled to light intensity. Higher H2O2 and antioxidant levels (guaiacol peroxidase, catalase (CAT), and superoxidase dismutase) were observed at 600 µmol·m−2·s−1 rather than at 300 µmol·m−2·s−1. Changes in antioxidant levels under each light condition differed between the species. Significant correlations were observed between antioxidant activities and H2O2 contents for both species, except for the CAT activity of P. ambiguum at 300 µmol·m−2·s−1. Under each of the conditions, both species responded proportionately to oxidative stress. Even under maximum light intensities (300 µmol·m−2·s−1 or 600 µmol·m−2·s−1 PAR intensity), neither species was stressed. Studies using extended exposure durations are warranted to better understand the growth performance and long-term physiological responses of both species.
Association between Active Helicobacter pylori Infection and Glaucoma: A Systematic Review and Meta-Analysis
Authors: Doulberis Michael , Papaefthymiou Apostolis , Polyzos Stergios A. , Bargiotas Panagiotis , Liatsos Christos , Srivastava David Shiva , Zavos Christos , Katsinelos Panagiotis , Kountouras Jannis
Published: June 2020

Background: Glaucoma is the second most common cause of blindness worldwide affecting almost 70 million individuals. Helicobacter pylori (H. pylori) is a widespread pathogen with systematic pathogenicity. This meta-analysis aimed to estimate the contradictory data regarding a potential association between active H. pylori infection and glaucoma. Materials and Methods: A research in MEDLINE/PubMed and Google Scholar was conducted and original studies investigating the relationship between H. pylori infection and glaucoma were included. Analysis was performed with random effects model. The main outcome was the odds ratio (OR) with 95% confidence intervals (CI) of H. pylori infection as a risk factor for glaucoma. A parallel analysis studied the role of active infection as indicated by histology and the titer of anti-H. pylori antibodies. For the anti-H. pylori antibody titers, weighted mean differences (WMD) were estimated between patients and controls. Results: Fifteen studies were included, with 2664 participants (872 patients with glaucoma and 1792 controls), divided into primary open-angle glaucoma (POAG), normal tension glaucoma (NTG) and pseudo-exfoliation glaucoma (PEG). The association between H. pylori infection and overall glaucoma was significant (OR = 2.08, CI 95% 1.48–2.93) with moderate heterogeneity (I2 = 61.54%). After stratification by glaucoma subtype, heterogeneity was eliminated in the NTG subgroup. Studies with healthy controls, and controls with anemia yielded very low or no heterogeneity, respectively. Gastric biopsy to document active H. pylori infection yielded the highest OR (5.4, CI: 3.17–9.2, p < 0.001) and null heterogeneity. For anti-H. pylori antibody titers, there was a significant difference in WMD between patients and controls (WMD 15.98 IU/mL; 95% CI: 4.09–27.87; p = 0.008); values were greater in glaucoma patients, with high heterogeneity (I2: 93.8%). Meta-regression analysis showed that mean age had a significant impact on glaucoma (p = 0.037). Conclusions: Active H. pylori infection may be associated with glaucoma with null heterogeneity, as, beyond histology, quantified by anti-H. pylori titers and increases with age.
Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods
Authors: Domokos Erzsébet , Bíró-Janka Béla , Bálint János , Molnár Katalin , Fazakas Csaba , Jakab-Farkas László , Domokos József , Albert Csilla , Mara Gyöngyvér , Balog Adalbert
Published: June 2020

Artemisinin extracted from Artemisia annua has been used efficiently in malaria treatment since 2005. In this study, the variations in plant parameters (plant biomass, glandular trichome density, essential oil total chemical content, artemisinin production, and polyphenol oxidase (PPO) activity) were tested under different soil types (Luvisol, Gleysol, Anthrosol and sterile peat) and cultivation conditions (potted plants in semi-open field, and open field experiments) for plants inoculated with arbuscular mycorrhizal fungus (AMF) Rizophagus irregularis. Under semi-open field conditions, the AMF colonization of A. annua plant roots varied, and presented the highest percentage in Luvisol and sterile peat. The increase in the root colonization rate positively influenced some plant parameters (biomass, glandular trichome density, artemisinin concentration, essential oil quantity and composition), but no effects on PPO enzyme activity were detected. AMF fungus R. irregularis significantly increased the artemisinin content and essential oil yield of plants cultivated in Luvisol, Gleysol, Anthrosol and in peat. These soil types can offer appropriate conditions for A. annua cultivation and artemisinin production even on a smaller scale. Under open field conditions, low (about 5%) AMF colonization was observed. No differences in artemisin contents were detected, but essential oil yield significantly increased compared to control plants. AMF treatment increased beta-farnesene and germacrene D concentrations in Artemisia plants in the open field experiment.
Digitocracy: Ruling and Being Ruled
Authors: Ballesteros Alfonso
Published: June 2020

Digitalisation is attracting much scholarly attention at present. However, scholars often take its benefits for granted, overlooking the essential question: “Does digital technology make us better?” This paper aims to help fill this gap by examining digitalisation as a form of government (digitocracy) and the way it shapes a new kind of man: animal digitalis. I argue that the digitalised man is animal-like rather than machine-like. This man does not use efficient and cold machine-like language, but is rather emotionalised through digital technology. If those who are ruled acted like machines, data would not be produced on a mass scale, and machine learning would stop learning. Digital man has animal features and is ruled by his brain’s reward system. We need to abandon this new form of government and the resulting man. To overcome digitalisation, we need a humanism that recovers the proper place of man over animals and artefacts, but maintains respect for the value of nature.
A Hybrid Network for Large-Scale Action Recognition from RGB and Depth Modalities
Authors: Wang Huogen , Song Zhanjie , Li Wanqing , Wang Pichao
Published: June 2020

The paper presents a novel hybrid network for large-scale action recognition from multiple modalities. The network is built upon the proposed weighted dynamic images. It effectively leverages the strengths of the emerging Convolutional Neural Network (CNN) and Recurrent Neural Network (RNN) based approaches to specifically address the challenges that occur in large-scale action recognition and are not fully dealt with by the state-of-the-art methods. Specifically, the proposed hybrid network consists of a CNN based component and an RNN based component. Features extracted by the two components are fused through canonical correlation analysis and then fed to a linear Support Vector Machine (SVM) for classification. The proposed network achieved state-of-the-art results on the ChaLearn LAP IsoGD, NTU RGB+D and Multi-modal & Multi-view & Interactive ( M 2 I ) datasets and outperformed existing methods by a large margin (over 10 percentage points in some cases).
Local Bearing Estimation for a Swarm of Low-Cost Miniature Robots
Authors: Liu Zheyu , West Craig , Lennox Barry , Arvin Farshad
Published: June 2020

Swarm robotics focuses on decentralised control of large numbers of simple robots with limited capabilities. Decentralised control in a swarm system requires a reliable communication link between the individuals that is able to provide linear and angular distances between the individuals—Range & Bearing. This study presents the development of an open-source, low-cost communication module which can be attached to miniature sized robots; e.g., Mona. In this study, we only focused on bearing estimation to mathematically model the bearings of neighbouring robots through systematic experiments using real robots. In addition, the model parameters were optimised using a genetic algorithm to provide a reliable and precise model that can be applied for all robots in a swarm. For further investigation and improvement of the system, an additional layer of optimisation on the hardware layout was implemented. The results from the optimisation suggested a new arrangement of the sensors with slight angular displacements on the developed board. The precision of bearing was significantly improved by optimising in both software level and re-arrangement of the sensors’ positions on the hardware layout.
Sensor-to-Segment Calibration Methodologies for Lower-Body Kinematic Analysis with Inertial Sensors: A Systematic Review
Authors: Pacher Léonie , Chatellier Christian , Vauzelle Rodolphe , Fradet Laetitia
Published: June 2020

Kinematic analysis is indispensable to understanding and characterizing human locomotion. Thanks to the development of inertial sensors based on microelectronics systems, human kinematic analysis in an ecological environment is made possible. An important issue in human kinematic analyses with inertial sensors is the necessity of defining the orientation of the inertial sensor coordinate system relative to its underlying segment coordinate system, which is referred to sensor-to-segment calibration. Over the last decade, we have seen an increase of proposals for this purpose. The aim of this review is to highlight the different proposals made for lower-body segments. Three different databases were screened: PubMed, Science Direct and IEEE Xplore. One reviewer performed the selection of the different studies and data extraction. Fifty-five studies were included. Four different types of calibration method could be identified in the articles: the manual, static, functional, and anatomical methods. The mathematical approach to obtain the segment axis and the calibration evaluation were extracted from the selected articles. Given the number of propositions and the diversity of references used to evaluate the methods, it is difficult today to form a conclusion about the most suitable. To conclude, comparative studies are required to validate calibration methods in different circumstances.
Quality Assessment of the Neural Algorithms on the Example of EIT-UST Hybrid Tomography
Authors: Kłosowski Grzegorz , Rymarczyk Tomasz , Cieplak Tomasz , Niderla Konrad , Skowron Łukasz
Published: June 2020

The paper presents the results of research on the hybrid industrial tomograph electrical impedance tomography (EIT) and ultrasonic tomography (UST) (EIT-UST), operating on the basis of electrical and ultrasonic data. The emphasis of the research was placed on the algorithmic domain. However, it should be emphasized that all hardware components of the hybrid tomograph, including electronics, sensors and transducers, have been designed and mostly made in the Netrix S.A. laboratory. The test object was a tank filled with water with several dozen percent concentration. As part of the study, the original multiple neural networks system was trained, the characteristic feature of which is the generation of each of the individual pixels of the tomographic image, using an independent artificial neural network (ANN), with the input vector for all ANNs being the same. Despite the same measurement vector, each of the ANNs generates its own independent output value for a given tomogram pixel, because, during training, the networks get their respective weights and biases. During the tests, the results of three tomographic methods were compared: EIT, UST and EIT-UST hybrid. The results confirm that the use of heterogeneous tomographic systems (hybrids) increases the reliability of reconstruction in various measuring cases, which is used to solve quality problems in managing production processes.
Low Temperature Adhesive Bonding-Based Fabrication of an Air-Borne Flexible Piezoelectric Micromachined Ultrasonic Transducer
Authors: Liu Wei , Wu Dawei
Published: June 2020

This paper presents the development of a flexible piezoelectric micromachined ultrasonic transducer (PMUT) that can conform to flat, concave, and convex surfaces and work in air. The PMUT consists of an Ag-coated polyvinylidene fluoride (PVDF) film mounted onto a laser-manipulated polymer substrate. A low temperature (<100 °C) adhesive bonding technique is adopted in the fabrication process. Finite element analysis (FEA) is implemented to confirm the capability of predicting the resonant frequency of composite diaphragms and optimizing the device. The manufactured PMUT exhibits a center frequency of 198 kHz with a wide operational bandwidth. Its acoustic performance is demonstrated by transmitting and receiving ultrasound in air on curved surface. The conclusions from this study indicate the proposed PMUT has great potential in ultrasonic and wearable devices applications.
Gait Analysis in a Box: A System Based on Magnetometer-Free IMUs or Clusters of Optical Markers with Automatic Event Detection
Authors: Marín Javier , Blanco Teresa , de la Torre Juan , Marín José J.
Published: June 2020

Gait analysis based on full-body motion capture technology (MoCap) can be used in rehabilitation to aid in decision making during treatments or therapies. In order to promote the use of MoCap gait analysis based on inertial measurement units (IMUs) or optical technology, it is necessary to overcome certain limitations, such as the need for magnetically controlled environments, which affect IMU systems, or the need for additional instrumentation to detect gait events, which affects IMUs and optical systems. We present a MoCap gait analysis system called Move Human Sensors (MH), which incorporates proposals to overcome both limitations and can be configured via magnetometer-free IMUs (MH-IMU) or clusters of optical markers (MH-OPT). Using a test–retest reliability experiment with thirty-three healthy subjects (20 men and 13 women, 21.7 ± 2.9 years), we determined the reproducibility of both configurations. The assessment confirmed that the proposals performed adequately and allowed us to establish usage considerations. This study aims to enhance gait analysis in daily clinical practice.