bluuebunny/crossref_metadata_2025 huggingface dataset:
Tianma Gouteng Decoction Exerts Pregnancy-Protective Effects Against Preeclampsia via Regulation of Oxidative Stress and NO Signaling
Authors: Jin Meiyuan , Cao Bin , Lin Chao , Li Jiayong , Xu Qiang , Ren Qianlei , Xu Shouying , Tang Chao
Published: March 2022

Preeclampsia (PE), a pregnancy-specific syndrome with the major molecular determinants of placenta-borne oxidative stress and consequently impaired nitric oxide (NO) generation, has been considered to be one of the leading causes of maternal morbidity as well as mortality and preterm delivery worldwide. Several medical conditions have been found to be associated with increased PE risk, however, the treatment of PE remains unclear. Here, we report that Tianma Gouteng Decoction (TGD), which is used clinically for hypertension treatment, regulates oxidative stress and NO production in human extravillous trophoblast-derived TEV-1 cells. In human preeclamptic placental explants, reactive oxygen species (ROS) levels were elevated and NO production was inhibited, while TGD treatment at different periods effectively down-regulated the H2O2-induced ROS levels and significantly up-regulated the H2O2-suppressed NO production in human TEV-1 cells. Mechanistically, TGD enhanced the activity of total nitric oxide synthase (TNOS), which catalyze L-arginine oxidation into NO, and simultaneously, TGD promoted the expression of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), two isoforms of nitric oxide synthetases (NOS) in human placenta, resulting in the increased NO generation. More importantly, TGD administration not only increased the weight gain during pregnancy and revealed a hypotensive effect, but also improved the placental weight gain and attenuated fetal growth restriction in an NG-nitro-L-arginine methyl ester (L-NAME)-induced mouse PE-like model. Our results thereby provide new insights into the role of TGD as a potentially novel treatment for PE.
The Endophytic Strain ZS-3 Enhances Salt Tolerance in Arabidopsis thaliana by Regulating Photosynthesis, Osmotic Stress, and Ion Homeostasis and Inducing Systemic Tolerance
Authors: Shi Li-Na , Lu Lan-Xiang , Ye Jian-Ren , Shi Hui-Min
Published: March 2022

Soil salinity is one of the main factors limiting agricultural development worldwide and has an adverse effect on plant growth and yield. To date, plant growth-promoting rhizobacteria (PGPR) are considered to be one of the most promising eco-friendly strategies for improving saline soils. The bacterium Bacillus megaterium ZS-3 is an excellent PGPR strain that induces growth promotion as well as biotic stress resistance and tolerance to abiotic stress in a broad range of host plants. In this study, the potential mechanisms of protection against salinity stress by B. megaterium ZS-3 in Arabidopsis thaliana were explored. Regulation by ZS-3 improved growth in A. thaliana under severe saline conditions. The results showed that ZS-3 treatment significantly increased the biomass, chlorophyll content and carotenoid content of A. thaliana. Compared to the control, the leaf area and total fresh weight of plants inoculated with ZS-3 increased by 245% and 271%, respectively; the chlorophyll a, chlorophyll b, and carotenoid contents increased by 335%, 146%, and 372%, respectively, under salt stress. Physiological and biochemical tests showed that ZS-3 regulated the content of osmotic substances in plants under salt stress. Compared to the control, the soluble sugar content of the ZS-3-treated group was significantly increased by 288%, while the proline content was significantly reduced by 41.43%. Quantification of Na+ and K+ contents showed that ZS-3 treatment significantly reduced Na+ accumulation and increased the K+/Na+ ratio in plants. ZS-3 also isolated Na+ in vesicles by upregulating NHX1 and AVP1 expression while limiting Na+ uptake by downregulating HKT1, which protected against Na+ toxicity. Higher levels of peroxidase and catalase activity and reduced glutathione were detected in plants inoculated with ZS-3 compared to those in uninoculated plants. In addition, it was revealed that ZS-3 activates salicylic acid (NPR1 and PR1) and jasmonic acid/ethylene (AOS, LOX2, PDF1.2, and ERF1) signaling pathways to induce systemic tolerance, thereby inducing salt tolerance in plants. In conclusion, the results of this study indicate that ZS-3 has the potential to act as an environmentally friendly salt tolerance inducer that can promote plant growth in salt-stressed environments.
Application of Educational Psychology-Based Dance Therapy in College Students’ Life Education
Authors: Zhong Haiyan , Zhao Chunhui , Zhang Fengrui , Zhang Ruizhi
Published: March 2022

The purpose is to strengthen the life education of contemporary college students and give better play to the vital role of life education in preventing college students’ mental diseases. Specifically, it discusses the role of dance therapy (DT) in College Students’ Life Education (CSLE). Firstly, based on educational psychology (EP), this manuscript analyzes the relevant theoretical concepts of EP and life education and discusses the importance of life education to contemporary college students. Secondly, following a Questionnaire Survey (QS) and using deep learning (DL) Convolutional Neural Network (CNN) and Facial Emotion Recognition (FER), this manuscript reviews and examines the CSLE’s current situation and the DT effect. Research findings are summarized combined with the QS results and scores of 20 subjects before and after five activities in 3 months. (I) After DT intervention, the positive dimensions of college students’ life values have improved, especially self-development and dedication, and their quality of life is refined. Thus, DT group counseling proves the positive role of DT in CSLE. (II) After DT intervention, 96.5% of the members think DT is effective. Therefore, EP-based DT is more effective and scientific in CSLE. The research findings provide a DT-based teaching concept for CSLE, explore the feasibility and effectiveness of life education, and enrich the DT scheme of CSLE. The research provides a practical reference for further applying DT in college students’ psychological education.
Intracranial Rosai Dorfman Disease Presented With Multiple Huge Intraventricular Masses: A Case Report
Authors: Jamali Elena , Sharifi Guive , Ghafouri-Fard Soudeh , Bidari Zerehpoosh Farahnaz , Yazdanpanahi Mahmood , Taheri Mohammad
Published: March 2022

Introduction

Rosai-Dorfman disease (RDD) usually presents with painless cervical lymphadenopathy during the two first decades of life, with or without extranodal involvement. Exclusive extranodal manifestation, and especially central nervous system (CNS) involvement, is uncommon. The etiology remains unknown and definitive diagnosis is based on characteristic histopathological and immunohistochemical features showing S100(+) CD1a(−) RDD type histiocytes with emperipolesis. Most CNS cases are dural-based masses.

Case Presentation

Herein, we present a case of RDD in an 8-year-old boy, who presented with multiple huge intraventricular masses.

Conclusion

Regarding rare CNS involvement and lack of established evidence-based therapeutic approaches, reports of any individual case can supply further beneficial data concerning treatment approaches and long-term effectiveness of therapeutic strategies.
Maintenance of Sertoli Cell Number and Function in Immature Human Testicular Tissues Exposed to Platinum-Based Chemotherapy—Implications for Fertility Restoration
Authors: Matilionyte Gabriele , Tharmalingam Melissa D. , Sanou Iris , Lopes Federica , Lane Sheila , Stukenborg Jan-Bernd , Spears Norah , Anderson Richard A. , Mitchell Rod T.
Published: March 2022

Background: Retrospective studies in adult survivors of childhood cancer show long-term impacts of exposure to alkylating chemotherapy on future fertility. We recently demonstrated germ cell loss in immature human testicular tissues following exposure to platinum-based chemotherapeutic drugs. This study investigated the effects of platinum-based chemotherapy exposure on the somatic Sertoli cell population in human fetal and pre-pubertal testicular tissues.

Methods: Human fetal (n = 23; 14–22 gestational weeks) testicular tissue pieces were exposed to cisplatin (0.5 or 1.0 μg/ml) or vehicle for 24 h in vitro and analysed 24–240 h post-exposure or 12 weeks after xenografting. Human pre-pubertal (n = 10; 1–12 years) testicular tissue pieces were exposed to cisplatin (0.5 μg/ml), carboplatin (5 μg/ml) or vehicle for 24 h in vitro and analysed 24–240 h post-exposure; exposure to carboplatin at 10-times the concentration of cisplatin reflects the relative clinical doses given to patients. Immunohistochemistry was performed for SOX9 and anti-Müllerian hormone (AMH) expression and quantification was carried out to assess effects on Sertoli cell number and function respectively. AMH and inhibin B was measured in culture medium collected post-exposure to assess effects on Sertoli cell function.

Results: Sertoli cell (SOX9+ve) number was maintained in cisplatin-exposed human fetal testicular tissues (7,647 ± 459 vs. 7,767 ± 498 cells/mm2; p > 0.05) at 240 h post-exposure. No effect on inhibin B (indicator of Sertoli cell function) production was observed at 96 h after cisplatin (0.5 and 1.0 μg/ml) exposure compared to control (21 ± 5 (0.5 μg/ml cisplatin) vs. 23 ± 7 (1.0 μg/ml cisplatin) vs. 25 ± 7 (control) ng/ml, p > 0.05). Xenografting of cisplatin-exposed (0.5 μg/ml) human fetal testicular tissues had no long-term effect on Sertoli cell number or function (percentage seminiferous area stained for SOX9 and AMH, respectively), compared with non-exposed tissues. Sertoli cell number was maintained in human pre-pubertal testicular tissues following exposure to either 0.5 μg/ml cisplatin (6,723 ± 1,647 cells/mm2) or 5 μg/ml carboplatin (7,502 ± 627 cells/mm2) compared to control (6,592 ± 1,545 cells/mm2).

Conclusions: This study demonstrates maintenance of Sertoli cell number and function in immature human testicular tissues exposed to platinum-based chemotherapeutic agents. The maintenance of a functional Sertoli cell environment following chemotherapy exposure suggests that fertility restoration by spermatogonial stem cell (SSC) transplant may be possible in boys facing platinum-based cancer treatment.
Bullying, Mental Health, and the Moderating Role of Supportive Adults: A Cross-National Analysis of Adolescents in 45 Countries
Authors: Kim Samuel Seunghan , Craig Wendy Marion , King Nathan , Bilz Ludwig , Cosma Alina , Molcho Michal , Qirjako Gentiana , Gaspar De Matos Margarida , Augustine Lilly , Šmigelskas Kastytis , Pickett William
Published: March 2022

Objectives: Relationships with supportive adults during adolescence may be a protective factor that lowers the risks associated with bullying. The current study aimed to examine the moderating role of supportive adults in the associations between bullying involvement (in-person and cyber) and mental health problems (psychological symptoms and low life satisfaction).

Methods: Data from 45 countries and regions taking part in the 2017/18 Health Behaviour in School-Aged Children study (N = 230,757) were used. Multivariable Poisson regression models were used to estimate relative risks of bullying on mental health. Effect estimates were compared across the number of supportive adults to examine a possible cumulative protective effect of relationships with supportive adults.

Results: Bullying involvement was consistently associated with poor mental health across the 45 countries. Risk of mental health problems associated with bullying involvement was greatest among students reporting relationships with multiple supportive adults. This was true for all indicators of bullying involvement.

Conclusion: Bullying remains a prevalent and harmful experience for youth worldwide. Merely having supportive adults is not sufficient in protecting youth from experiencing the mental health risks associated with bullying.
Factors Associated to Psychological Distress During the COVID-19 Pandemic Among Healthcare Workers in Ecuador
Authors: Ruiz-Frutos Carlos , Arias-Ulloa Cristian Arturo , Ortega-Moreno Mónica , Romero-Martín Macarena , Escobar-Segovia Kenny F. , Adanaque-Bravo Ingrid , Gómez-Salgado Juan
Published: March 2022

Objective: The global COVID-19 pandemic has challenged health systems. Healthcare professionals had to face harsh conditions that have caused psycho-emotional consequences. Ecuador has been one of the countries hit hardest by the pandemic in Latin America. The objective of this study was to analyse the levels of psychological distress among healthcare workers in Ecuador during the COVID-19 pandemic.

Methods: A cross-sectional descriptive study was conducted with a convenience sample of 1,056 healthcare professionals, assessing their psychological distress, physical symptoms of COVID-19, state of health, the preventive measures adopted, and the history of contact with people infected with the SARS-CoV2 virus.

Results: showed that 66.0% of the participants manifested psychological distress, with significantly higher levels in women with symptoms of COVID-19 and previous contact with infected people or objects (p < 0.001). However, adherence to preventive measures and perception of health were associated with less psychological distress (p < 0.001).

Conclusions: The importance of monitoring the mental health of healthcare workers during the COVID-19 pandemic was confirmed, having identified factors associated with the development of psychological distress among professionals in Ecuador.
Multi-Fidelity Sparse Polynomial Chaos and Kriging Surrogate Models Applied to Analytical Benchmark Problems
Authors: Rumpfkeil Markus P. , Bryson Dean , Beran Phil
Published: March 2022

In this article, multi-fidelity kriging and sparse polynomial chaos expansion (SPCE) surrogate models are constructed. In addition, a novel combination of the two surrogate approaches into a multi-fidelity SPCE-Kriging model will be presented. Accurate surrogate models, once obtained, can be employed for evaluating a large number of designs for uncertainty quantification, optimization, or design space exploration. Analytical benchmark problems are used to show that accurate multi-fidelity surrogate models can be obtained at lower computational cost than high-fidelity models. The benchmarks include non-polynomial and polynomial functions of various input dimensions, lower dimensional heterogeneous non-polynomial functions, as well as a coupled spring-mass-system. Overall, multi-fidelity models are more accurate than high-fidelity ones for the same cost, especially when only a few high-fidelity training points are employed. Full-order PCEs tend to be a factor of two or so worse than SPCES in terms of overall accuracy. The combination of the two approaches into the SPCE-Kriging model leads to a more accurate and flexible method overall.
Multi-Objective Design of a Distributed Ducted Fan System
Authors: Guo Jiahao , Zhou Zhou
Published: March 2022

The distributed propulsion system applied to the vertical take-off and landing aircraft must maintain the high performance in both hover and cruise flight. The gap of the power unit has an adverse effect on the distributed ducted fan system, especially in cruise flight. Therefore, a new distributed ducted fan system was proposed, which eliminated the power gap in design, and adjusted the contraction and expansion of the wake through the deflectable induced wing arranged behind the ducted fan to ensure the high efficiency of the distributed ducted fan system in different flight phases. Then, a multi-objective design method of the distributed ducted fan system was proposed, and the feasibility of the design method was verified by designing the inlet and outlet of the duct and the induced wing. Design results show that the performance change of the distributed ducted fan system mainly came from the change of the inlet. By increasing the length and height of the inlet, the flow separation was alleviated and the duct thrust was increased in hove flight, but the cruise drag became larger. The increase of the inlet height made the operating point of the blade far away in hover and cruise flight, which increased the difficulty of the multi-objective design. Compared with the distributed ducted fan system composed of the traditional circular ducted fan, the hovering power load was reduced by 3.703%, but the cruise efficiency was increased by 17.372%, and the spanwise space was reduced by 20% in the final design.
Preliminary Investigation of Mixed Orchard Hays on the Meat Quality, Fatty Acid Profile, and Gastrointestinal Microbiota in Goat Kids
Authors: Wang Yingying , Li Tengfei , Chen Xinyi , Liu Chongyi , Jin Xumei , Tan Hua , Long Mingxiu
Published: March 2022

This preliminary investigation was designed to study the effects of different mixed orchard hays on meat quality, fatty acids, amino acids, rumen intestinal microflora, and the relationship between rumen bacteria and fatty acids in the longissimus dorsi muscle of Saanen dairy goats. In this preliminary investigation, goats were separately fed crop straws (corn and wheat straws) and alfalfa (Medicago sativa L.) (CK group), alfalfa + oats (Avena sativa L.) (group I), alfalfa + perennial ryegrass (Lolium perenne L.) (group II), and hairy vetch (Vicia villosa Roth.) + perennial ryegrass (group III). There were differences in shear force and cooking loss between treatments. The contents of saturated fatty acids (SFAs) C14:0, C16:0, and C18:0 in the CK group were significantly higher than those in other three groups (p < 0.001). The 16S rDNA sequencing results showed that the relative abundance of Proteobacteria in group II were higher than those in other three groups (p < 0.05). Association analysis showed that Prevotella_1 was negatively correlated with C18:0 and significantly positively correlated with C16:1, while Clostridium and Romboutsia showed a positive correlation with monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs). Therefore, feeding mixed hays can increase beneficial fatty acids and the percentages of associated bacteria in rumen and intestines.
Antimicrobial Random Peptide Mixtures Eradicate Acinetobacter baumannii Biofilms and Inhibit Mouse Models of Infection
Authors: Caraway Hannah E. , Lau Jonathan Z. , Maron Bar , Oh Myung Whan , Belo Yael , Brill Aya , Malach Einav , Ismail Nahed , Hayouka Zvi , Lau Gee W.
Published: March 2022

Antibiotic resistance is one of the greatest crises in human medicine. Increased incidents of antibiotic resistance are linked to clinical overuse and overreliance on antibiotics. Among the ESKAPE pathogens, Acinetobacter baumannii, especially carbapenem-resistant isolates, has emerged as a significant threat in the context of blood, urinary tract, lung, and wound infections. Therefore, new approaches that limit the emergence of antibiotic resistant A. baumannii are urgently needed. Recently, we have shown that random peptide mixtures (RPMs) are an attractive alternative class of drugs to antibiotics with strong safety and pharmacokinetic profiles. RPMs are antimicrobial peptide mixtures produced by incorporating two amino acids at each coupling step, rendering them extremely diverse but still defined in their overall composition, chain length, and stereochemistry. The extreme diversity of RPMs may prevent bacteria from evolving resistance rapidly. Here, we demonstrated that RPMs rapidly and efficiently kill different strains of A. baumannii, inhibit biofilm formation, and disrupt mature biofilms. Importantly, RPMs attenuated bacterial burden in mouse models of acute pneumonia and soft tissue infection and significantly reduced mouse mortality during sepsis. Collectively, our results demonstrate RPMs have the potential to be used as powerful therapeutics against antibiotic-resistant A. baumannii.
Delineation of the Flooded Areas in Urban Environments Based on a Simplified Approach
Authors: Dinu Cristian , Sîrbu Nicolai , Drobot Radu
Published: March 2022

The Tulcea municipality is frequently exposed to rainfall-generated floods, with its lower downtown area (located in the Danube’ former meadow, now protected by dikes) being flooded two to three times per year. In this study, our objective was to understand the generation mechanism of these rainfall-triggered urban floods and to identify tailored mitigation options. Due to the lack of reliable information on the sewage network (diameters, slope, material) and the uncertain sewage outflows during heavy rain events, a rather simplified approach was preferred by the authors of this paper. The data processing was performed using GIS tools, with appropriate accounting of the digital terrain modelling, ortho-photos, administrative boundaries of the Tulcea municipality, delineation of the urban catchments, imagery of the frequently flooded areas, and the urban sewage network. Subsequently, a fast hydrological modelling and a volume-based flooding approach were developed in order to identify and evaluate the flooded urban areas under extreme rainfall events. Upon the completion of the calibration and validation processes, numerical simulations were run that considered the design storms of different return periods. Due to the high slopes of the hills, hence the short concentration time of the pluvial waters, a Sponge City approach does not seem as though it would be easy to implement. A more efficient solution utilising large-capacity buried urban retention tanks in the lower part of the municipality was alternatively identified. Further on, this solution will be supported by a set of green measures.
The Influence of Soaking and Sprouting on the Physicochemical Characteristics of Tigernut Tubers (Cyperus esculentus L.)
Authors: Djomdi Djomdi , Bakari Hamadou , Gibert Olivier , Tran Thierry , Ejoh Richard , Christophe Gwendoline , Michaud Philippe , Ndjouenkeu Robert
Published: March 2022

The influence of soaking and germination on the physicochemical characteristics of tigernut tubers (Cyperus esculentus L.) are investigated. Tubers (Ø > 1 cm) were soaked in an ascorbic acid solution (0.1%) for 48 h at 40 °C before undergoing germination for 6 days. The textural profile, the capacity and germination energy and the biochemical composition of these tubers were determined. The germination energy varied from 76.18 to 79.48% for the quantities of solution of 24 and 48 mL, respectively. The textural profile of the tubers varied depending on the type of treatment. Germination resulted in a considerable reduction in amylose content from 14.15% for the native tigernut tuber to 9.98% for the sprouted one. This treatment also increased the protein, ascorbic acid and ash contents, which ranged from 7.54 to 8.82 g/100 g DM, 250 to 275.39 mg/100 g DM and 2.60 to 3.84 g/100 g DM, respectively. The starch content of the tubers following germination remained high, which could come up against the pasteurization of the milk from these tubers.
Numerical and Experimental Studies of Free-Fall Drop Impact Tests Using Strain Gauge, Piezoceramic, and Fiber Optic Sensors
Authors: Dimino Ignazio , Diodati Gianluca , Di Caprio Francesco , Ciminello Monica , Menichino Aniello , Inverno Michele , Belardo Marika , Di Palma Luigi
Published: March 2022

The present work is framed inside a broader activity aimed at improving the accuracy of numerical models in predicting the crashworthiness behavior of flexible fuel tanks. This paper describes a comprehensive experimental and numerical study aimed at estimating the impact force of a test article, consisting of a soft nylon bag filled with water, subjected to crash impact tests. In order to understand and improve response predictions, the test article drops freely from different heights, and then strikes onto a rigid plate which is instrumented with different types of sensors. Strain gauges, piezoceramic sensors, and fiber optics are used to measure the strain induced by the impact force during the experiments. To tune the test matrix and the measurement chain parameters, numerical computations are carried out to predict the dynamics of drop impact through FE explicit analyses. Through analysis and comparison with experimental results, a relationship between strain and impact energy correlated with the drop height is established, and the overall accuracy of the entire measurement chain is assessed to determine the effectiveness of such a methodology in a full-scale test on a flexible fuel tank structure.
Heterogeneity of Synchronous Lung Metastasis Calls for Risk Stratification and Prognostic Classification: Evidence from a Population-Based Database
Authors: Wang Shuncong , Chen Lei , Feng Yuanbo , Swinnen Johan V. , Jonscher Charles , Van Ongeval Chantal , Ni Yicheng
Published: March 2022

The epidemiology and associated potential heterogeneity of synchronous lung metastasis (sLM) have not been reported at a population-based level. Cancer patients with valid information about sLM status in the Surveillance, Epidemiology, and End Results database were enrolled. The prevalence of sLM, with a 95% confidential interval, and median survival of sLM, with interquartile range, were calculated and compared by Chi-square analyses and log-rank tests by primary cancer type and clinicopathological factors. Furthermore, the risk factors of sLM development were identified by multivariate logistic regression. Among 1,672,265 enrolled cases, 3.3% cases were identified with sLM, with a median survival of 7 months. Heterogeneity in prevalence and prognosis in sLM was observed among different primary cancers, with the highest prevalence in main bronchus cancer and best survival in testis cancer. Higher prevalence and poorer prognosis were observed in the older population, male population, African American, patients with lower socioeconomic status, and cases with advanced T stage, N stage, or more malignant pathological characteristics. Race, age, T stage, N stage, metastasis to other sites, insurance status and marital status were associated with sLM development (p < 0.001). The current study highlights the heterogeneity of the prevalence and prognosis in patients with sLM.
Impact of Histotripsy on Development of Intrahepatic Metastases in a Rodent Liver Tumor Model
Authors: Worlikar Tejaswi , Zhang Man , Ganguly Anutosh , Hall Timothy L. , Shi Jiaqi , Zhao Lili , Lee Fred T. , Mendiratta-Lala Mishal , Cho Clifford S. , Xu Zhen
Published: March 2022

Histotripsy has been used for tumor ablation, through controlled, non-invasive acoustic cavitation. This is the first study to evaluate the impact of partial histotripsy ablation on immune infiltration, survival outcomes, and metastasis development, in an in vivo orthotopic, immunocompetent rat HCC model (McA-RH7777). At 7–9 days post-tumor inoculation, the tumor grew to 5–10 mm, and ~50–75% tumor volume was treated by ultrasound-guided histotripsy, by delivering 1–2 cycle histotripsy pulses at 100 Hz PRF (focal peak negative pressure P– >30 MPa), using a custom 1 MHz transducer. Complete local tumor regression was observed on MRI in 9/11 histotripsy-treated rats, with no local recurrence or metastasis up to the 12-week study end point, and only a <1 mm residual scar tissue observed on histology. In comparison, 100% of untreated control animals demonstrated local tumor progression, developed intrahepatic metastases, and were euthanized at 1–3 weeks. Survival outcomes in histotripsy-treated animals were significantly improved compared to controls (p-value < 0.0001). There was evidence of potentially epithelial-to-mesenchymal transition (EMT) in control tumor and tissue healing in histotripsy-treated tumors. At 2- and 7-days post-histotripsy, increased immune infiltration of CD11b+, CD8+ and NK cells was observed, as compared to controls, which may have contributed to the eventual regression of the untargeted tumor region in histotripsy-treated tumors.
The Psychometric Properties of the Trunk Impairment Scale in Children with Cerebral Palsy
Authors: Jung Hyerim , Choi Young-Eun
Published: March 2022

The Trunk Impairment Scale (TIS) measures static and dynamic seated trunk control in children with cerebral palsy (CP) who have postural control problems. Studies have investigated the reliability and validity of the TIS. However, the fitness and difficulty of the scale items have not been investigated. This study used Rasch analysis to test the construct validity of TIS for children with CP. TIS data were collected from 60 children with CP and analyzed for person and item fit, item difficulty, rating scale suitability, and separation reliability. Principal component analyses of residuals revealed that TIS had unidimensionality. Five misfit items (static sitting balance (SSB) items 2 and 3, dynamic sitting balance (DSB) items 4 and 5, and coordination (COO) item 3) were identified. DSB8 is the most difficult item, followed by DSB3 and COO4. On the other hand, the SSB3 item was found to be a relatively easy item. The rating scales demonstrated that out of the three subscales, SSB, DSB, and COO, only the SSB subscale did not meet the appropriate criteria. We demonstrated that statistical item analysis with the Rasch model could provide valuable information related to psychometric properties.
Group Theory of Syntactical Freedom in DNA Transcription and Genome Decoding
Authors: Planat Michel , Amaral Marcelo M. , Fang Fang , Chester David , Aschheim Raymond , Irwin Klee
Published: March 2022

Transcription factors (TFs) are proteins that recognize specific DNA fragments in order to decode the genome and ensure its optimal functioning. TFs work at the local and global scales by specifying cell type, cell growth and death, cell migration, organization and timely tasks. We investigate the structure of DNA-binding motifs with the theory of finitely generated groups. The DNA ‘word’ in the binding domain—the motif—may be seen as the generator of a finitely generated group Fdna on four letters, the bases A, T, G and C. It is shown that, most of the time, the DNA-binding motifs have subgroup structures close to free groups of rank three or less, a property that we call ‘syntactical freedom’. Such a property is associated with the aperiodicity of the motif when it is seen as a substitution sequence. Examples are provided for the major families of TFs, such as leucine zipper factors, zinc finger factors, homeo-domain factors, etc. We also discuss the exceptions to the existence of such DNA syntactical rules and their functional roles. This includes the TATA box in the promoter region of some genes, the single-nucleotide markers (SNP) and the motifs of some genes of ubiquitous roles in transcription and regulation.
Evaluation of Long-Term Trends of Rainfall in Perak, Malaysia
Authors: Hanif Muhammad Faisal , Mustafa Muhammad Raza Ul , Liaqat Muhammad Usman , Hashim Ahmad Mustafa , Yusof Khamaruzaman Wan
Published: March 2022

This study aimed to examine the spatiotemporal seasonal and annual trends of rainfall indices in Perak, Malaysia, during the last 35 years, as any seasonal or spatial variability in rainfall may influence the regional hydrological cycle and water resources. Mann–Kendall and Sequential Mann–Kendall (SMK) tests were used to assess seasonal and annual trends. Precipitation concentration index was used to estimate variations in rainfall concentration, and Theil–Sen’s slope estimator was used to determine the spatial variability of rainfall. It was found that most of the rainfall indices are showing decreasing trends, and it was most prominent for the southwest monsoon season with a decreasing rate of 2.20 mm/year. The long-term trends for seasonal rainfall showed that rainfall declined by 0.29 mm/year during the southwest monsoon. In contrast, the northeast and the inter-monsoon seasons showed slight increases. Rainfall decreased gradually from 1994 to 2008, and the trend became more pronounced in 2008. On a spatial basis, rainfall trends have shifted from the western regions (i.e., −19 mm/year) to the southeastern regions (i.e., 10 mm/year). Overall, slightly decreasing trends in rainfall were observed in Perak Malaysia.
High-Resolution Estimation of Monthly Air Temperature from Joint Modeling of In Situ Measurements and Gridded Temperature Data
Authors: Wilson Bradley , Porter Jeremy R. , Kearns Edward J. , Hoffman Jeremy S. , Shu Evelyn , Lai Kelvin , Bauer Mark , Pope Mariah
Published: March 2022

Surface air temperature is an important variable in quantifying extreme heat, but high-resolution temporal and spatial measurement is limited by sparse climate-data stations. As a result, hyperlocal models of extreme heat involve intensive physical data collection efforts or analyze satellite-derived land-surface temperature instead. We developed a geostatistical model that integrates in situ climate-quality temperature records, gridded temperature data, land-surface temperature estimates, and spatially consistent covariates to predict monthly averaged daily maximum surface-air temperatures at spatial resolutions up to 30 m. We trained and validated the model using data from North Carolina. The fitted model showed strong predictive performance with a mean absolute error of 1.61 ∘F across all summer months and a correlation coefficient of 0.75 against an independent hyperlocal temperature model for the city of Durham. We show that the proposed model framework is highly scalable and capable of producing realistic temperature fields across a variety of physiographic settings, even in areas where no climate-quality data stations are available.
Effect of Glycerol, Calcium and Transglutaminase Post-Treatment on the Properties of Regenerated Fibers from Rennet-Treated Casein Micelles
Authors: Thill Sebastian , Gebhardt Ronald
Published: March 2022

Regenerated fibers can be prepared from a cooled solution of renneted casein micelles in a wet spinning process. For better handling and stability of the fiber, plasticizers, network modifiers or cross-linkers are used in the production process. For that reason, fibers with different glycerol and calcium content are prepared in this study and subsequently treated with the enzyme transglutaminase before being characterized after air drying. In addition to the swelling behavior in NaOH, H2O, simulated milk ultrafiltrate buffer as well as HCl, the mechanical properties of the fibers are investigated, taking into account their microscopic fine structure. Transglutaminase-treated fibers show sigmoidal absorption curves for all solvents and reach higher equilibrium swelling percentages than untreated fibers. When the calcium content in the coagulation bath is increased from 50 mM to 100 mM, more stabilizing calcium bridges lead to a denser fiber structure that swells more slowly in all solvents considered. With increasing glycerol content, the flexibility of the fibers increases, as indicated by the decrease in elastic moduli, and a fine structure in the sub-µm range becomes visible. The fibers also demonstrate lower elastic moduli when post-treated with transglutaminase. Besides the higher casein content due to the transglutaminase treatment, this could also contribute to the higher equilibrium swelling percentages compared to the untreated fibers.
Perspective on Cancer Control: Whither the Tobacco Endgame for Canada?
Authors: Eisenhauer Elizabeth A. , Schwartz Robert , Cunningham Rob , Hagen Les , Fong Geoffrey T. , Callard Cynthia , Chaiton Michael , Pipe Andrew
Published: March 2022

Aims: In 2014, in response to evidence that Canada’s tobacco use would lead, inexorably, to substantial morbidity and mortality for the foreseeable future, a group of experts convened to consider the development of a “Tobacco Endgame” for Canada. The “Tobacco Endgame” defines a time frame in which to eliminate structural, political, and social dynamics that sustain tobacco use, leading to improved population health. Strategies: A series of Background Papers describing possible measures that could contribute to the creation of a comprehensive endgame strategy for Canada was prepared in advance of the National Tobacco Endgame Summit hosted at Queen’s University in 2016. At the summit, agreement was reached to work together to achieve <5% tobacco use by 2035 (<5 by ’35). A report of the proceedings was shared widely. Achievements: Progress since 2016 has been mixed. The Summit report was followed by a national forum convened by Health Canada in March 2017, and in 2018, the Canadian Government adopted “<5 × ’35” tobacco use target in a renewed Canadian tobacco reduction strategy. Tobacco use has declined in the last 5 years, but at a rate slower than that which will be needed to achieve the <5 by ’35 goal. There remain > 5 million smokers in Canada, signaling that smoking-related diseases will continue to be an enormous health burden. Furthermore, the landscape of new products (e-cigarettes and cannabis) has created additional risks and opportunities. Future directions: A bold, reinvigorated tobacco control strategy is needed that significantly advances ongoing policy developments, including full implementation of the key demand-reduction policies of the WHO Framework Convention on Tobacco Control. Formidable, new disruptive policies and regulations will be needed to achieve Canada’s Endgame goal.
Ibrutinib in c-MYC and HER2 Amplified Oesophagogastric Carcinoma: Results of the Proof-of-Concept iMYC Study
Authors: Turkes Fiona , Bryant Annette , Begum Ruwaida , Davidson Michael , Kalaitzaki Eleftheria , Aresu Maria , Lazaro-Alcausi Retchel , Bryant Jane , Rana Isma , Chua Sue , Aronson Lauren , Hulkki-Wilson Sanna , Fribbens Charlotte , Watkins David , Rao Sheela , Starling Naureen , Cunningham David , Chong Irene Y. , Chau Ian
Published: March 2022

Oesophagogastric (OG) cancer is a highly lethal disease requiring novel treatment options. c-MYC and/or HER-2 amplified oesophageal cancer models have demonstrated sensitivity to BTK inhibition with ibrutinib. We evaluated the safety and efficacy of ibrutinib in patients with c-MYC and/or HER2 amplified pre-treated advanced OG cancer. c-MYC and HER2 amplification status were determined by FISH. The primary endpoint was overall response rate (ORR). Secondary endpoints were disease control rate (DC) at 8 weeks, safety, progression-free survival (PFS) and overall survival (OS). Eleven patients were enrolled. Eight patients had c-MYC amplified tumours, six were HER2 amplified and three were c-MYC and HER2 co-amplified. Grade ≥ 3 adverse events were fever, neutropenia, and vomiting. Grade ≥ 3 gastrointestinal haemorrhage occurred in three patients and was fatal in two cases. Among seven evaluable patients, three patients (43%) achieved a best response of SD at 8 weeks. No PR or CR was observed. Disease control was achieved for 32 weeks in one patient with a dual c-MYC and HER2 highly co-amplified tumour. The median PFS and OS were 1.5 (95% CI: 0.8–5.1) and 5.1 (95% CI: 0.8–14.5) months, respectively. Ibrutinib had limited clinical efficacy in patients with c-MYC and/or HER2 amplified OG cancer. Unexpected gastrointestinal bleeding was observed in 3 out of 8 treated patients which was considered a new safety finding for ibrutinib in this population.
The Utility of Endoscopic-Ultrasonography-Guided Tissue Acquisition for Solid Pancreatic Lesions
Authors: Tanaka Hiroki , Matsusaki Shimpei
Published: March 2022

Endoscopic-ultrasonography-guided tissue acquisition (EUS-TA) has been widely performed for the definitive diagnosis of solid pancreatic lesions (SPLs). As the puncture needles, puncture techniques, and sample processing methods have improved, EUS-TA has shown higher diagnostic yields and safety. Recently, several therapeutic target genomic biomarkers have been clarified in pancreatic ductal carcinoma (PDAC). Although only a small proportion of patients with PDAC can benefit from precision medicine based on gene mutations at present, precision medicine will also be further developed for SPLs as more therapeutic target genomic biomarkers are identified. Advances in next-generation sequencing (NGS) techniques enable the examination of multiple genetic mutations in limited tissue samples. EUS-TA is also useful for NGS and will play a more important role in determining treatment strategies. In this review, we describe the utility of EUS-TA for SPLs.
Adaptive Hurst-Sensitive Active Queue Management
Authors: Marek Dariusz , Szyguła Jakub , Domański Adam , Domańska Joanna , Filus Katarzyna , Szczygieł Marta
Published: March 2022

An Active Queue Management (AQM) mechanism, recommended by the Internet Engineering Task Force (IETF), increases the efficiency of network transmission. An example of this type of algorithm can be the Random Early Detection (RED) algorithm. The behavior of the RED algorithm strictly depends on the correct selection of its parameters. This selection may be performed automatically depending on the network conditions. The mechanisms that adjust their parameters to the network conditions are called the adaptive ones. The example can be the Adaptive RED (ARED) mechanism, which adjusts its parameters taking into consideration the traffic intensity. In our paper, we propose to use an additional traffic parameter to adjust the AQM parameters—degree of self-similarity—expressed using the Hurst parameter. In our study, we propose the modifications of the well-known AQM algorithms: ARED and fractional order PIαDβ and the algorithms based on neural networks that are used to automatically adjust the AQM parameters using the traffic intensity and its degree of self-similarity. We use the Fluid Flow approximation and the discrete event simulation to evaluate the behavior of queues controlled by the proposed adaptive AQM mechanisms and compare the results with those obtained with their basic counterparts. In our experiments, we analyzed the average queue occupancies and packet delays in the communication node. The obtained results show that considering the degree of self-similarity of network traffic in the process of AQM parameters determination enabled us to decrease the average queue occupancy and the number of rejected packets, as well as to reduce the transmission latency.
Coupled VAE: Improved Accuracy and Robustness of a Variational Autoencoder
Authors: Cao Shichen , Li Jingjing , Nelson Kenric P. , Kon Mark A.
Published: March 2022

We present a coupled variational autoencoder (VAE) method, which improves the accuracy and robustness of the model representation of handwritten numeral images. The improvement is measured in both increasing the likelihood of the reconstructed images and in reducing divergence between the posterior and a prior latent distribution. The new method weighs outlier samples with a higher penalty by generalizing the original evidence lower bound function using a coupled entropy function based on the principles of nonlinear statistical coupling. We evaluated the performance of the coupled VAE model using the Modified National Institute of Standards and Technology (MNIST) dataset and its corrupted modification C-MNIST. Histograms of the likelihood that the reconstruction matches the original image show that the coupled VAE improves the reconstruction and this improvement is more substantial when seeded with corrupted images. All five corruptions evaluated showed improvement. For instance, with the Gaussian corruption seed the accuracy improves by 1014 (from 10−57.2 to 10−42.9) and robustness improves by 1022 (from 10−109.2 to 10−87.0). Furthermore, the divergence between the posterior and prior distribution of the latent distribution is reduced. Thus, in contrast to the β-VAE design, the coupled VAE algorithm improves model representation, rather than trading off the performance of the reconstruction and latent distribution divergence.
A Soar-Based Space Exploration Algorithm for Mobile Robots
Authors: Luo Fei , Zhou Qin , Fuentes Joel , Ding Weichao , Gu Chunhua
Published: March 2022

Space exploration is a hot topic in the application field of mobile robots. Proposed solutions have included the frontier exploration algorithm, heuristic algorithms, and deep reinforcement learning. However, these methods cannot solve space exploration in time in a dynamic environment. This paper models the space exploration problem of mobile robots based on the decision-making process of the cognitive architecture of Soar, and three space exploration heuristic algorithms (HAs) are further proposed based on the model to improve the exploration speed of the robot. Experiments are carried out based on the Easter environment, and the results show that HAs have improved the exploration speed of the Easter robot at least 2.04 times of the original algorithm in Easter, verifying the effectiveness of the proposed robot space exploration strategy and the corresponding HAs.
Task’s Choice: Pruning-Based Feature Sharing (PBFS) for Multi-Task Learning
Authors: Chen Ying , Yu Jiong , Zhao Yutong , Chen Jiaying , Du Xusheng
Published: March 2022

In most of the existing multi-task learning (MTL) models, multiple tasks’ public information is learned by sharing parameters across hidden layers, such as hard sharing, soft sharing, and hierarchical sharing. One promising approach is to introduce model pruning into information learning, such as sparse sharing, which is regarded as being outstanding in knowledge transferring. However, the above method performs inefficiently in conflict tasks, with inadequate learning of tasks’ private information, or through suffering from negative transferring. In this paper, we propose a multi-task learning model (Pruning-Based Feature Sharing, PBFS) that merges a soft parameter sharing structure with model pruning and adds a prunable shared network among different task-specific subnets. In this way, each task can select parameters in a shared subnet, according to its requirements. Experiments are conducted on three benchmark public datasets and one synthetic dataset; the impact of the different subnets’ sparsity and tasks’ correlations to the model performance is analyzed. Results show that the proposed model’s information sharing strategy is helpful to transfer learning and superior to the several comparison models.
The Influence of Different Operating Conditions on the Support Bracket Stress in Pumped Storage Units
Authors: Xu Buchao , Zhao Weiqiang , Lin Wenhua , Mao Zhongyu , Tao Ran , Wang Zhengwei
Published: March 2022

In order to balance the intermittent supply of energy to the power grid, pumped storage units have to operate more and more in extended operating conditions and switch their mode frequently. During operation, the turbine unit has to withstand various axial forces that may cause deformation and fatigue damage to the key components of the machine. The excessive load could surpass the weight of the runner body, which is dangerous for the power plant. For the safe and stable operation, the simulation of the axial force under pump condition is performed by the computational fluid dynamics method (CFD) in this paper. The CFD simulation result has revealed the variation rule of the axial force with the operating condition. Besides, the conditions with pressure-balance pipelines (PBP) blockage are also investigated and the mechanism of PBP on reducing the axial force applied on the bracket has been revealed. The maximum stresses are calculated by means of Finite Element Method (FEM) and compared with the normal conditions. The result shows that the blocked PBP will increase 62.20% of the maximum stress on the support bracket.