bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Accumulation of Metabolites in Suaeda Species Provides New Insights into Abiotic Stress Tolerance in C4-Halophytic Species in Elevated CO2 Conditions
Authors: Yadav Sonam , Elansary Hosam O. , Mattar Mohamed A. , M. Elhindi Khalid , A. Alotaibi Majed , Mishra Avinash
Published: January 2021

Halophytic plants can adapt to grow and thrive in highly saline conditions. Suaeda species are annual halophytes with high salt tolerance and are most suitable in the restoration of salinized or contaminated saline land and as food, forage, medicine, and bioenergy. In this study, we comprehensively analyzed the different metabolic responses of Suaeda species under salt and drought stress at ambient and elevated CO2 conditions. Seedlings of Suaeda species were treated with 500 mM NaCl and 5% of polyethylene glycol under elevated CO2 stress conditions for 24 h. Then, widely untargeted metabolites were detected by gas chromatography–mass spectrometry. Different metabolites involved in amino acid metabolism, glycolysis, photorespiration, and tricarboxylic acid cycle were quantitatively determined after stress treatments. A total of 61 primary metabolites were annotated. Different treatments increased the contents of certain metabolites, such as amino acids, sugars, and organic acids, as well as some antioxidants, such as quininic acid, kaempferol, and melatonin. These substances may be correlated with osmotic tolerance, increased antioxidant activity, and medical and nutritional value in the species. This study suggests that various metabolites differentially accumulated in C4Suaeda species under varying stress conditions. Furthermore, this work provides new insights into the key secondary metabolite pathway involved in stress tolerance.
Morphological Divergence of Hermann’s Tortoise (Testudo hermanni boettgeri Mojsisovits, 1889) in Albania
Authors: Duro Sokol , Sönmez Bektaş , Gündemir Ozan , Jashari Tefik , Szara Tomasz
Published: January 2021

Testudines show phenotypic plasticity, and variation among specific populations within a species is widespread. Morphological differences between populations may reflect ecological factors that drive adaptation to local conditions. In this context, we gathered basic data on the morphology of the Hermann’s tortoise (Testudo hermanni boettgeri Mojsisovits, 1889) to document their variation across different geographical regions. We surveyed Hermann’s tortoises in five different locales within Albania during April and May 2020 and measured 20 morphological characteristics, including carapace and plastron dimensions. We measured 188 tortoises (81 males, 107 females) in this study, and females were larger (p = 0.0001) and heavier (p = 0.0001) than males. Mean straight carapace length (SCL) and body mass were 172.4 mm and 1128.8 g, respectively, for females, and 151.3 mm and 735 g, respectively, for males. The Albanian T. h. boettgeri were regionally diverged into three different populations that were situated in northern (Shkodra), central (Tirana, Berati, and Ballshi), and southern (Saranda) Albania. The body size (curved carapace length (CCL)) of females was positively correlated (r = 0.216; p = 0.025) with the latitude, in accordance with Bergmann’s rule. However, there was no correlation between body size and latitude in males. These striking regional differences among Albanian T. h. boettgeri strongly suggest that further study of molecular variations and reproductive output of Hermann’s tortoises is warranted.
Albumen Quality of Fresh and Stored Table Eggs: Hen Genotype as a Further Chance for Consumer Choice
Authors: Rizzi Chiara
Published: January 2021

The quality of fresh (1 d) and stored (7–14–21 d, 21 °C) eggs was studied in Italian dual-purpose breeds (Ermellinata di Rovigo (ER), Robusta maculata (RM)) and hybrids (Hy-Line Brown (HB), Hy-Line White36 (HW)), reared outdoors (4 m2/bird) and fed commercial feed. The eggs were analyzed at 4 ages, throughout different seasonal environmental conditions, from summer (31, 35 weeks; 25 °C) until autumn (39, 43 weeks, 15 °C). Each genotype showed significant (p < 0.01) changes in egg quality. In 1 d eggs, the eggshell thickness changed in RM and HW (quadratic), decreased linearly in ER; Haugh Units (HU) changed (ER–cubic) and decreased (hybrids-linear). In 7 d and 14 d eggs, HU linearly (p < 0.01) decreased, except in RM. In 21 d eggs, HU (ER linear decrease; HB, HW quadratic) changed. Significant negative correlations between albumen pH and height were seen in ER (at 1 d, 14 d, 21 d) and HW (at each storage time) eggs, and in RM and HB only in 1 d eggs. RM showed a quite stable albumen quality and a lower total egg mass than ER which showed a more variable albumen quality, due also to a lower eggshell thickness and shape index. The hybrids produced a higher total egg mass than the purebreds and showed an intermediate variation of the egg quality, with an albumen quality higher than those of ER and RM only in 1 d egg, as a result of a higher albumen weight.
The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets
Authors: Tomaszewska Ewa , Burmańczuk Natalia , Dobrowolski Piotr , Świątkiewicz Małgorzata , Donaldson Janine , Burmańczuk Artur , Mielnik-Błaszczak Maria , Kuc Damian , Milewski Szymon , Muszyński Siemowit
Published: January 2021

The effect of alpha-ketoglutaric acid (AKG) supplementation to experimentally-induced, perinatal growth-retarded piglets was examined. Sows were treated with a synthetic glucocorticoid (Gc) during the last 25 days of pregnancy, and after the birth, piglets were randomly divided into three groups depending on the treatment. The Gc/Gc + AKG and Gc/AKG groups born by Gc-treated sows after the birth were treated with Gc or Gc + AKG for 35 days. Significantly lower serum growth hormone, IGF-I, osteocalcin, leptin, and cortisol concentrations were observed in the Gc/Gc + AKG group, while the bone alkaline phosphatase activity was significantly higher. Serum insulin concentration was higher in the control group. Serum alanine, lysine, histidine, and tryptophan concentrations were higher in the Gc/Gc + AKG and Gc/AKG groups. The perinatal action of Gc significantly affects histomorphometry of articular cartilage and trabecular bone and bone mechanics. The results clearly showed that dietary AKG had positive effects with regards to the profile of free amino acids. Taking into account the function of AKG as an energy donor and stimulator of collagen synthesis, it can be concluded that the anabolic role of AKG may be the main mechanism responsible for its protective effect against the GC-induced perinatal intensified catabolic state.
Feed Restriction Induced Changes in Behavior, Corticosterone, and Microbial Programming in Slow- and Fast-Growing Chicken Breeds
Authors: Yan Chao , Xiao Jinlong , Chen Di , Turner Simon P. , Li Zhiwei , Liu Hao , Liu Wen , Liu Jian , Chen Siyu , Zhao Xingbo
Published: January 2021

This study aimed to explore the difference between two Chinese local broilers, one slow- and one fast-growing, in their response to a stress challenge. We conducted the study on slow- (Weining chicken) and fast-growing (Jinlinghua chicken) breeds, with 50 chickens from each breed either feed restricted to 70% for 30 days as a stress or given ad libitum to evaluate the effects on behavior, corticosterone, and microbial programming. Standing behavior was more frequent while exploration was less common in fast-growing breeds compared to slow-growing breeds. Food seeking and ingestion, exploration, and drinking increased, while resting decreased in the feed restricted treatments. There was no difference in corticosterone concentration between slow- and fast-growing chickens, but the level was affected by feeding treatments, and the interaction of breed and feed restriction. At the genus-level, the relative abundance of Bacteroides and Lactobacillus was higher, while Cloacibacillus and Megasphaera was lower in the slow-growing breed compared to the fast-growing breed. Feed restricted birds had a higher abundance of Mucispirillum, but lower abundance of Cloacibacillus, Clostridium XlVa and Clostridium IV. In conclusion, feed restriction to 70% for 30 days as a chronic stress stimulation caused more activity, elevated the stress response, and altered gut microbiota composition, but some changes were only evident in slow- or fast-growing chickens.
Appraising the Welfare of Thoroughbred Racehorses in Training in Queensland, Australia: The Incidence, Risk Factors and Outcomes for Horses after Retirement from Racing
Authors: Crawford Kylie L. , Finnane Anna , Greer Ristan M. , Phillips Clive J. C. , Woldeyohannes Solomon M. , Perkins Nigel R. , Ahern Benjamin J.
Published: January 2021

There is international public concern regarding retirement of racehorses, including the reason for retirement and the outcome for horses after racing. However, there are currently no prospective studies investigating these factors. A recent independent inquiry in Queensland, Australia, highlighted that the true outcomes for horses after retirement from racing are largely unknown. Furthermore, there are currently no measures to monitor the outcome for racehorses and their welfare once they have left the care of the trainer. This study investigated these gaps in knowledge through a weekly survey conducted over a 13-month period. We aimed to evaluate: (1) the incidence of retirement, (2) the reasons and risk factors for retirement and (3) the medium-term (greater than 6 months) outcomes for horses after retirement. Data were collected through personal structured weekly interviews with participating trainers and analysed using negative binomial and logistic regression. There was a low incidence of retirements, namely 0.4% of horses in training per week. The season and training track did not affect the incidence of retirement. Musculoskeletal injuries were the most common reason for retirement (40/110 horses, 36%). Involuntary retirements accounted for 56/100 (51%) of retirements, whereby musculoskeletal injuries, respiratory or cardiac conditions and behavioural problems prevented the horse from racing The odds of voluntary retirement, whereby the horse was retired due to racing form or impending injury, increased with each additional race start (OR 1.05; p = 0.01) and start/year of racing (OR 1.21; p = 0.03) but decreased with increasing percentage of first, second and third places (OR 0.94; p < 0.001). Medium-term follow-up (median 14 months, IQR 11, 18, range 8–21) revealed that most horses (108/110; 98%) were repurposed after retirement, almost half as performance horses (50/110; 46%). Horses that voluntarily retired had 2.28 times the odds of being repurposed as performance horses than those retired involuntarily (p = 0.03). Whether retirement was voluntary or involuntary did not influence whether horses were used for breeding or pleasure. The primary limitation of this study is that our results reflect retirement in racehorses in South East Queensland, Australia, and may not be globally applicable. Furthermore, we were unable to monitor the long-term outcome and welfare of horses in their new careers. It is vital that the industry is focused on understanding the risks for voluntary rather than involuntary retirement and optimising the long-term repurposing of horses. There is a need for traceability and accountability for these horses to ensure that their welfare is maintained in their new careers.
Glial Response and Neuroinflammation in Cerebrocortical Atrophy in a Young Irish Wolfhound Dog
Authors: Sant’Ana Fabiano J. F. de , Omaña Miguel , Blasco Ester , Pumarola Martí
Published: January 2021

A two-year-old, Irish Wolfhound dog presented with a history of progressive neurological signs. Neurological exam revealed disorientation, absence of menace response, reduction of right nasal sensation, hypermetria and ataxia with reduction of proprioception in all four limbs. MRI findings were compatible with laminar neuronal necrosis and possible bilateral cortical cerebral atrophy. Grossly, a severe bilateral reduction of the gray matter with flattening of gyri, mainly in frontal and parietal cerebral areas, was observed. Histologically, multiple, segmental, bilateral, and symmetric areas of neuronal loss, necrosis and degeneration, in a laminar pattern, associated with a reactive gliosis were observed. Immunohistochemical studies showed severe reduction of neuronal bodies, proliferation and hypertrophy of astrocytes and microglia. Few perivascular B and T cells were demonstrated. Based on these data, we show some of the neuroinflammatory events that occur during CNS repair in a chronic phase of this condition.
MyFishCheck: A Model to Assess Fish Welfare in Aquaculture
Authors: Tschirren Linda , Bachmann David , Güler Ali Cem , Blaser Oliver , Rhyner Nicola , Seitz Andreas , Zbinden Erich , Wahli Thomas , Segner Helmut , Refardt Dominik
Published: January 2021

Welfare in animal husbandry includes considerations of biology, ethics, ecology, law and economics. These diverse aspects must be translated into common quantifiable parameters and applicable methods to objectively assess welfare in animals. To assist this process in the field of aquaculture, where such methods are largely missing, we developed a model to assess fish welfare. A network of information was created to link needs, i.e., fundamental requirements for welfare, with parameters, i.e., quantifiable aspects of welfare. From this ontology, 80 parameters that are relevant for welfare, have practicable assessment methods and deliver reliable results were selected and incorporated into a model. The model, named MyFishCheck, allows the evaluation of welfare in five distinct modules: farm management, water quality, fish group behaviour, fish external and fish internal appearance, thereby yielding five individual grades categorising welfare ranging from critical, to poor, to acceptable, and good. To facilitate the use of the model, a software application was written. With its adaptability to different fish species, farming systems, regulations and purposes as well as its user-friendly digital version, MyFishCheck is a next step towards improved fish welfare assessment and provides a basis for ongoing positive developments for the industry, the farmers and the fish.
Growth Performance and Clinicopathological Analyses in Lambs Repetitively Inoculated with Aluminum-Hydroxide Containing Vaccines or Aluminum-Hydroxide Only
Authors: de Miguel Ricardo , Asín Javier , Rodríguez-Largo Ana , Echeverría Irache , Lacasta Delia , Pinczowski Pedro , Gimeno Marina , Molín Jéssica , Fernández Antonio , de Blas Ignacio , de Andrés Damián , Pérez Marta , Reina Ramsés , Luján Lluís
Published: January 2021

Aluminum (Al) hydroxide is an effective adjuvant used in sheep vaccines. However, Al-adjuvants have been implicated as potential contributors to a severe wasting syndrome in sheep—the so-called ovine autoimmune-inflammatory syndrome induced by adjuvants (ASIA syndrome). This work aimed to characterize the effects of the repetitive injection of Al-hydroxide containing products in lambs. Four flocks (Flocks 1–4; n = 21 each) kept under different conditions were studied. Three groups of seven lambs (Vaccine, Adjuvant-only, and Control) were established in each flock. Mild differences in average daily gain and fattening index were observed, indicating a reduced growth performance in Vaccine groups, likely related to short-term episodes of pyrexia and decreased daily intake. Clinical and hematological parameters remained within normal limits. Histology showed no significant differences between groups, although there was a tendency to present a higher frequency of hyperchromatic, shrunken neurons in the lumbar spinal cord in the Adjuvant-only group. Although Al-hydroxide was linked to granulomas at the injection site and behavioral changes in sheep, the results of the present experimental work indicate that injected Al-hydroxide is not enough to fully reproduce the wasting presentation of the ASIA syndrome. Other factors such as sex, breed, age, production system, diet or climate conditions could play a role.
The Laetiporus sulphureus Fermented Product Enhances the Antioxidant Status, Intestinal Tight Junction, and Morphology of Broiler Chickens
Authors: Lin Wei Chih , Lee Tzu Tai
Published: January 2021

The Laetiporus sp. is a fungal species that is traditionally used for medicinal purposes. This study investigated the effects of the Laetiporus sulphureus fermented product (FL) as a feed supplementation on the antioxidant activities, the intestinal Tight Junction (TJ) mRNA expression, and the intestinal morphology of broiler chickens. Four-hundred one-day-old male broilers (Ross 308) were randomly allocated to five experimental diets: (1) a corn-soybean meal basal diet (control), (2) a basal diet replaced with 5% Wheat Bran (5% WB), (3) a basal diet replaced with 10% WB (10% WB), (4) a basal diet replaced with 5% FL (5% FL), and (5) a basal diet replaced with 10% FL (10% FL). The FL-supplemented groups exhibited a better feed conversion ratio in the overall experimental period compared to the WB and control groups. The serum antioxidant profiles of 35-day-old broilers showed that, compared to the control and 10% WB groups, the 5% FL supplementation group had a significantly increased superoxide dismutase activity, while it down-regulated the concentration of malondialdehyde in the serum (p < 0.05). The assessment of selected antioxidant gene expression showed that the 5% FL group significantly elevated heme oxygenase-1 and nuclear factor erythroid 2–related factor 2 expression, compared to the control and WB groups (p < 0.05). Furthermore, both of the FL supplemented groups had a significantly higher expression of glutathione peroxidase and catalase, compared to that of the WB and control groups in the jejunum (p < 0.05). The TJ mRNA expression in the jejunum showed that 5% FL significantly elevated the zonula occludens-1, claudin-1, and mucin-2 expression (p < 0.05), while 5% and 10% FL supplementation significantly improved OCLN expression in both the jejunum and ileum, compared to control group (p < 0.05). The intestinal morphology of 35-day-old broilers showed that a 5% FL supplementation significantly increased the villus height in the ileum and jejunum, compared to the WB and control groups (p < 0.05). Moreover, the 5% and 10% FL supplementation groups had a significantly higher villi:crypt ratio in the ileum, compared to the WB and control groups (p < 0.05). To conclude, FL supplementation improved the antioxidative status, the TJ mRNA expression, and the intestinal morphology, and it was accompanied by a lowered feed conversion ratio in broilers. Finally, 5% supplementation had the overall best results in improving the antioxidant status, TJ mRNA expression, and intestinal morphology of broilers.
Nannochloropsis spp. as Feed Additive for the Pacific White Shrimp: Effect on Midgut Microbiology, Thermal Shock Resistance and Immunology
Authors: Guimarães Ariane Martins , Guertler Cristhiane , do Vale Pereira Gabriella , da Rosa Coelho Jaqueline , Costa Rezende Priscila , Nóbrega Renata Oselame , do Nascimento Vieira Felipe
Published: January 2021

This work aimed to evaluate Nannochloropsis spp. as feed additive in the diet of Pacific white shrimp for their effect on midgut microbiology, thermal shock resistance and immunological parameters. Initially, the digestibility of the microalgae meal was assessed, and the apparent digestibility coefficient (ADC) was determined. The ADC was, in general, high in lipids (78.88%) and eicosapentaenoic fatty acid (73.86%). Then, Nannochloropsis spp. were included in diets at four levels (0, 0.5, 1 and 2% inclusion). The shrimp were reared in 500 L clear water tanks containing 20 shrimp per tank with an initial weight of 6.05 ± 0.06 g and fed four times a day. Shrimp fed with supplemented diets containing Nannochloropsis spp. (0.5 and 2%) presented higher resistance to thermal shock when compared to the non-supplemented group (control). Shrimp fed with 1 and 2% of algae inclusion had a higher production of reactive oxygen species (ROS) when compared to other treatments. No statistical difference was observed in the immunological parameters and microbiology of the intestinal tract. Thus, the inclusion of Nannochloropsis spp. in shrimp diets at 0.5 and 2% levels increases resistance to thermal shock and ROS production in shrimp.
Population Structure Assessed Using Microsatellite and SNP Data: An Empirical Comparison in West African Cattle
Authors: Álvarez Isabel , Fernández Iván , Traoré Amadou , Menéndez-Arias Nuria A. , Goyache Félix
Published: January 2021

A sample of 185 West African cattle belonging to nine different taurine, sanga, and zebu populations was typed using a set of 33 microsatellites and the BovineHD BeadChip of Illumina. The information provided by each type of marker was summarized via clustering methods and principal component analyses (PCA). The aim was to assess differences in performance between both marker types for the identification of population structure and the projection of genetic variability on geographical maps. In general, both microsatellites and Single Nucleotide Polymorphism (SNP) allowed us to differentiate taurine cattle from zebu and sanga cattle, which, in turn, would form a single population. Pearson and Spearman correlation coefficients computed among the admixture coefficients (fitting K = 2) and the eigenvectors corresponding to the first two factors identified using PCA on both microsatellite and SNP data were statistically significant (most of them having p < 0.0001) and high. However, SNP data allowed for a better fine-scale identification of population structure within taurine cattle: Lagunaire cattle from Benin were separated from two different N’Dama cattle samples. Furthermore, when clustering analyses assumed the existence of two parental populations only (K = 2), the SNPs could differentiate a different genetic background in Lagunaire and N’Dama cattle. Although the two N’Dama cattle populations had very different breeding histories, the microsatellite set could not separate the two N’Dama cattle populations. Classic bidimensional dispersion plots constructed using factors identified via PCA gave different shapes for microsatellites and SNPs: plots constructed using microsatellite polymorphism would suggest the existence of weakly differentiated, highly intermingled, subpopulations. However, the projection of the factors identified on synthetic maps gave comparable images. This would suggest that results on population structuring must be interpreted with caution. The geographic projection of genetic variation on synthetic maps avoids interpretations that go beyond the results obtained, particularly when previous information on the analyzed populations is scant. Factors influencing the performance of the projection of genetic parameters on geographic maps, together with restrictions that may affect the election of a given type of markers, are discussed.
The Occurrence of Antimicrobial-Resistant Salmonella enterica in Hatcheries and Dissemination in an Integrated Broiler Chicken Operation in Korea
Authors: Shang Ke , Wei Bai , Cha Se-Yeoun , Zhang Jun-Feng , Park Jong-Yeol , Lee Yea-Jin , Jang Hyung-Kwan , Kang Min
Published: January 2021

Positive identification rates of Salmonella enterica in hatcheries and upstream breeder farms were 16.4% (36/220) and 3.0% (6/200), respectively. Among the Salmonella serovars identified in the hatcheries, S. enterica ser. Albany (17/36, 47.2%) was the most prevalent, followed by the serovars S. enterica ser. Montevideo (11/36, 30.6%) and S. enterica ser. Senftenberg (5/36, 13.9%), which were also predominant. Thirty-six isolates showed resistance to at least one antimicrobial tested, of which 52.8% (n = 19) were multidrug resistant (MDR). Thirty-three isolates (enrofloxacin, MIC ≥ 0.25) showed point mutations in the gyrA and parC genes. One isolate, S. enterica ser. Virchow, carrying the blaCTX-M-15 gene from the breeder farm was ceftiofur resistant. Pulsed-field gel electrophoresis (PFGE) showed that 52.0% S. enterica ser. Montevideo and 29.6% S. enterica ser. Albany isolates sourced from the downstream of hatcheries along the broiler chicken supply chain carried the same PFGE types as those of the hatcheries. Thus, the hatcheries showed a high prevalence of Salmonella isolates with high antimicrobial resistance and no susceptible isolate. The AMR isolates from hatcheries originating from breeder farms could disseminate to the final retail market along the broiler chicken supply chain. The emergence of AMR Salmonella in hatcheries may be due to the horizontal spread of resistant isolates. Therefore, Salmonella control in hatcheries, particularly its horizontal transmission, is important.
Numerical Study of Turbid Slab Optical Properties Reconstruction from Multiple Scattering Signals Using Time-Based Markov Chain Model
Authors: Pan Hujie , Xu Qinglin , Li Xuesong , Wang Shangning , Xu Min
Published: January 2021

The reconstruction of optical properties for opaque mediums is highly desired for medical, atmosphere and aerosol applications. However, the modeling and reconstruction process is highly related with multiple scattering phenomena, which elevates both the complexity and computational costs for such efforts. This work introduces a time-based Markov chain method, which uses a sparse transition matrix to represent the likelihood for a photon to transit in the turbid media. The accuracy of the time-based Markov chain model was verified against the forwarding calculations of the scattering-based Markov chain model and Monte Carlo simulations. Then, reconstruction was performed with backscattered photon angular distributions. Based on the characteristics of the sparse transition matrix, the optical properties (droplet diameters) could be obtained layer by layer with transmitted photon distributions at different time durations. It is shown that the time-based Markov chain model can reconstruct the optical properties of a turbid slab with satisfactory accuracy and lower computational costs.
Combined Investigation of Indoor Climate Parameters and Energy Performance of a Winery
Authors: Panaras Giorgos , Tzimas Panagiotis , Tolis Evangelos I. , Papadopoulos Giannis , Afentoulidis Aristeidis , Souliotis Manolis
Published: January 2021

Wineries present significant interest on a research level, combining Indoor Air Quality (IAQ) issues related with substances emitted through the wine production, as well as the need for minimizing conventional energy consumption (optimizing energy performance). In the proposed work, experimental and theoretical analyses are presented which aim to achieve both targets, that of improved indoor climate and energy performance. An extensive measurement campaign was implemented, regarding indoor climate thermal parameters, as well as concentration of substances (CO2, VOCs, NO2) affecting IAQ. The results of the parameters were exploited for the assessment of indoor climate; moreover, data from indoor thermal parameters together with values of specific parameters related to the efficiency of the individual devices were utilized in the development of the energy model. The model was used to formulate and evaluate proposals for reducing the energy consumption of the winery. The proposals include the use of Renewable Energy Sources (RES) and, in particular, the installation of a photovoltaic array on the roof of the premises. Finally, an economic and technical study was carried out to determine the performance of the suggested interventions and the expected payback period.
Accuracy Verification of an Anatomical Model Manufactured Using Low-Cost Additive Production
Authors: Tóth Teodor , Varga Patrik , Štefanovič Branko , Bednarčíková Lucia , Schnitzer Marek , Hudák Radovan , Živčák Jozef
Published: January 2021

The paper deals with the separation of the third cervical vertebra using the software VGStudio MAX, Mimics, and inVesalius. During the separation, various parameters of the threshold were used to determine the effect. The comparison of models from Mimics and inVesalius to VGStudio MAX showed that the cumulative variance distribution for 95% surface coverage is less than 0.935 mm. When comparing medically oriented software, Mimics and inVesalius, the deviation was less than 0.356 mm. The model was made of polylactic acid (PLA) material on a low-cost 3D printer, Prusa i3 MK2.5 MMU1. The printed model was scanned by four scanners: Artec Eva, 3Shape D700, Steinbichler Comet L3D, and Creaform EXAscan. The outputs from the scanners were compared to the reference model (standard tessellation language (STL) model for 3D printing) as well as to the scanner with the best accuracy (3Shape). Compared to the publications below, the analysis of deviations was evaluated on the entire surface of the model and not on selected dimensions. The cumulative variance distribution for comparing the output from the 3D scanner with the reference model, as well as comparing the scanners, shows that the deviation for 95% of the surface coverage is at the level of 0.300 mm. Since the model of the vertebra is planned for education and training, the used software and technologies are suitable for use in the design and the production process.
Fructans with Varying Degree of Polymerization Enhance the Selective Growth of Bifidobacterium animalis subsp. lactis BB-12 in the Human Gut Microbiome In Vitro
Authors: Van den Abbeele Pieter , Duysburgh Cindy , Ghyselinck Jonas , Goltz Shellen , Berezhnaya Yulia , Boileau Thomas , De Blaiser Anke , Marzorati Massimo
Published: January 2021

Synbiotics aim to improve gastrointestinal health by combining pre- and probiotics. This study evaluated combinations of Bifidobacterium animalis subsp. lactis BB-12 with seven fructans: oligofructoses (OF1-OF2; low degree of polymerization (DP)), inulins (IN1-IN2-IN3; high DP) and OF/IN mixtures (OF/IN1-OF/IN2). During monoculture incubations, all fructans were fermented by BB-12 as followed from increased BB-12 numbers and increased acetate and lactate concentrations, with most pronounced fermentation for low DP fructans (OF1-OF2). Further, short-term colonic incubations for three human donors revealed that also in presence of a complex microbiota, all fructans (particularly OF1) consistently selectively enhanced the growth of BB-12. While each fructan as such already increased Bifidobacteriaceae numbers with 0.94–1.26 log(cells/mL), BB-12 co-supplementation additionally increased Bifidobacteriaceae with 0.17–0.46 log(cells/mL). Further, when co-supplemented with fructans, BB-12 decreased Enterobacteriaceae numbers (significant except for IN1-IN3). At metabolic level, all fructans decreased pH due to increased acetate and lactate production, while OF/IN2-IN1-IN2-IN3 also stimulated propionate and butyrate production. BB-12 co-supplementation further increased propionate and butyrate for OF/IN2-IN3 and IN1-IN2, respectively. Overall, combinations of BB-12 with fructans are promising synbiotic concepts, likely due to intracellular consumption of low DP-fructans by BB-12 (either present in starting product or released upon fermentation by indigenous microbes), thereby enhancing effects of the co-administered fructan.
Geothermal Assessment of Target Formations Using Recorded Temperature Measurements for the Alberta No. 1 Geothermal Project
Authors: Huang Katherine , Hickson Catherine , Cotterill Darrell , Champollion Yannick
Published: January 2021

The Alberta No. 1 project is a planned power and heat (direct use) geothermal project located within the County of Grande Prairie and Municipal District of Greenview. For the project to successfully produce power and heat on a commercial scale, temperatures of 120 °C are desirable. The produced fluids must also be from highly permeable formations from depths of less than 4500 m. Bottomhole temperature measurements and wireline logs from Alberta’s extensive oil and gas database were used to determine the depths to target formations and temperatures within these formations in the project area. The target formations include the dolomitized carbonate units of Devonian age from the Beaverhill Lake Group to the top of the Precambrian Basement. Permeable Devonian-aged sandstone units such as the Granite Wash Formation are also targets. Results suggest that elevation to the top of the Beaverhill Lake Group range from 3104 m to 4094 m and temperatures at the top of the formation range from 87 °C to 123 °C in the study area. Elevation to the top of the Precambrian Basement ranges from 3205 m to 4223 m and temperatures at the formation top range from 74 °C to 124 °C. Within the area where Alberta No. 1 plans to drill, temperatures close to and exceeding 120 °C are expected within the target formations.
Special Finite Elements with Adaptive Strain Field on the Example of One-Dimensional Elements
Authors: Chyży Tadeusz , Mackiewicz Monika
Published: January 2021

The conception of special finite elements called multi-area elements for the analysis of structures with different stiffness areas has been presented in the paper. A new type of finite element has been determined in order to perform analyses and calculations of heterogeneous, multi-coherent, and layered structures using fewer finite elements and it provides proper accuracy of the results. The main advantage of the presented special multi-area elements is the possibility that areas of the structure with different stiffness and geometrical parameters can be described by single element integrated in subdivisions (sub-areas). The formulation of such elements has been presented with the example of one-dimensional elements. The main idea of developed elements is the assumption that the deformation field inside the element is dependent on its geometry and stiffness distribution. The deformation field can be changed and adjusted during the calculation process that is why such elements can be treated as self-adaptive. The application of the self-adaptation method on strain field should simplify the analysis of complex non-linear problems and increase their accuracy. In order to confirm the correctness of the established assumptions, comparative analyses have been carried out and potential areas of application have been indicated.
Biodegradable and Compostable Shopping Bags under Investigation by FTIR Spectroscopy
Authors: Alfei Silvana , Schito Anna Maria , Zuccari Guendalina
Published: January 2021

In the recent years, plastic-based shopping bags have become irregular and progressively replaced by compostable ones. To be marketed, these “new plastics” must possess suitable requirements verified by specific bodies, which grant the conformity mark, and the approved physicochemical properties are periodically verified. The fast, inexpensive, non-destructive, easy to use, and reproducible Fourier-Transform infrared (FTIR) spectroscopy is a technique routinely applied to perform analysis in various industrial sectors. To get reliable information from spectral data, chemometric methods, such as Principal Component Analysis (PCA), are commonly suggested. In this context, PCA was herein performed on 4, 5, and 21 × 3251 matrices, collecting the FTIR data from regular and irregular shopping bags, including three freshly extruded films from the Italian industry MecPlast, to predict their compliance with legislation. The results allowed us to unequivocally achieve such information and to classify the bags as suitable for containing fresh food in bulk or only for transport. A self-validated linear model was developed capable to estimate, by acquiring a single FTIR spectrum if, after the productive process, the content of renewable poly-lactic-acid (PLA) in a new produced film respect the expectations. Surprisingly, our findings established that among the grocery bags available on the market, irregular plastic-based shopping bags continue to survive.
Delamination Detection in Bimetallic Composite Using Laser Ultrasonic Bulk Waves
Authors: Ji Baoping , Zhang Qingdong , Cao Jianshu , Zhang Boyang , Zhang Liyuan
Published: January 2021

In this study, a method based on laser ultrasonic bulk waves is used to detect delamination in a bimetallic composite. For this purpose, several artificial delamination defects were created in a copper-aluminum sample using wire-electrode cutting. The research includes numerical simulation and experimental analysis. The propagation process of laser ultrasonic in Cu/Al bimetallic compo-site, the interaction between bulk waves and composite interface, and the effect of delamination defects on the ultrasound field were studied by numerical simulation. Suitable parameters and features were determined by numerical simulation, which provided a basis for the parameter se-lection of experimental research. The reflected shear waves from the composite interface can act as a sensitive feature to detect the delamination in Cu/Al bimetallic composites. The distance between the detection point and the excitation point was set to 2 mm to take into account the detection resolution and efficiency. The experimental results were in good agreement with the simulation results, and the C-scan image can intuitively show the location and size of delamination defects. The detection method based on laser ultrasonic bulk waves can effectively detect the delamination in Cu/Al bimetallic composite, which is suitable for the on-line detection of the rolling composite process.
The Double Face of Metals: The Intriguing Case of Chromium
Authors: Genchi Giuseppe , Lauria Graziantonio , Catalano Alessia , Carocci Alessia , Sinicropi Maria Stefania
Published: January 2021

Chromium (Cr) is a common element in the Earth’s crust. It may exist in different oxidation states, Cr(0), Cr(III) and Cr(VI), with Cr(III) and Cr(VI) being relatively stable and largely predominant. Chromium’s peculiarity is that its behavior relies on its valence state. Cr(III) is a trace element in humans and plays a major role in glucose and fat metabolism. The beneficial effects of Cr(III) in obesity and types 2 diabetes are known. It has been long considered an essential element, but now it has been reclassified as a nutritional supplement. On the other hand, Cr(VI) is a human carcinogen and exposure to it occurs both in occupational and environmental contexts. It induces also epigenetic effects on DNA, histone tails and microRNA; its toxicity seems to be related to its higher mobility in soil and swifter penetration through cell membranes than Cr(III). The microorganisms Acinetobacter sp. Cr1 and Pseudomonas sp. Cr13 have been suggested as a promising agent for bioremediation of Cr(VI). This review intends to underline the important role of Cr(III) for human health and the dangerousness of Cr(VI) as a toxic element. The dual and opposing roles of this metal make it particularly interesting. An overview of the recent literature is reported in support.
Innovatively Fused Deep Learning with Limited Noisy Data for Evaluating Translations from Poor into Rich Morphology
Authors: Mouratidis Despoina , Kermanidis Katia Lida , Sosoni Vilelmini
Published: January 2021

Evaluation of machine translation (MT) into morphologically rich languages has not been well studied despite its importance. This paper proposes a classifier, that is, a deep learning (DL) schema for MT evaluation, based on different categories of information (linguistic features, natural language processing (NLP) metrics and embeddings), by using a model for machine learning based on noisy and small datasets. The linguistic features are string based for the language pairs English (EN)–Greek (EL) and EN–Italian (IT). The paper also explores the linguistic differences that affect evaluation accuracy between different kinds of corpora. A comparative study between using a simple embedding layer (mathematically calculated) and pre-trained embeddings is conducted. Moreover, an analysis of the impact of feature selection and dimensionality reduction on classification accuracy has been conducted. Results show that using a neural network (NN) model with different input representations produces results that clearly outperform the state-of-the-art for MT evaluation for EN–EL and EN–IT, by an increase of almost 0.40 points in correlation with human judgments on pairwise MT evaluation. It is observed that the proposed algorithm achieved better results on noisy and small datasets. In addition, for a more integrated analysis of the accuracy results, a qualitative linguistic analysis has been carried out in order to address complex linguistic phenomena.
Clinical Assessment of Dental Implants Placed in Low-Quality Bone Sites Prepared for the Healing Chamber with Osseodensification Concept: A Double-Blind, Randomized Clinical Trial
Authors: Mello-Machado Rafael Coutinho , de Almeida Barros Mourão Carlos Fernando , Javid Kayvon , Ferreira Henrique T. , Montemezzi Pietro , Calasans-Maia Mônica Diuana , Senna Plinio Mendes
Published: January 2021

The present study aimed to compare the stability of dental implants placed in low-quality bone prepared for the healing chamber with osseodensification technique and a standard undersized drilling. Sixteen subjects presenting D3 or D4 bone density according to Misch’s classification were randomly distributed to receive dental implants following either osseodensification (G1: n = 29) or standard undersized drilling (G2: n = 26) preparation techniques. Implant insertion torque (IT) and implant stability quotient (ISQ) were measured immediately after implant placement. Implant survival and secondary stability (ISQ) were evaluated after six months. The G1 group showed higher IT (39.0 ± 6.4 Ncm) than G2 (32.0 ± 3.4 Ncm) (p < 0.001). ISQ values were similar (p > 0.05) at the implant insertion (67.1 ± 3.2 and 65.5 ± 2.7 for G1 and G2, respectively). After six months healing, implant survival was equally comparable in both groups (p > 0.05), and ISQ values were higher than those of implant insertion (p < 0.001) but similar (p > 0.05) for both groups (74.0 ± 3.6 and 73.3 ± 3.2 for G1 and G2, respectively). Within the limitations of this study, the present RCT demonstrated that a wider surgical bed prepared by osseodensification instrumentation allowed for the bone healing-chamber concept in low-quality bone without any reduction in implant stability and success rate.
Assessment of Laser Joining Quality by Visual Inspection, Computer Simulation, and Deep Learning
Authors: Han Chang Min , Kim Tae Won , Choi Hae Woon
Published: January 2021

Polymer joining results are evaluated and compared in different ways, such as visual inspection, computer simulation, and deep learning analysis, to assess the joining quality. For the experiments, energies in the range of 3 to 5 J/mm were used from preliminary experimental data. A total of 15 welding experiment schedules were performed. Weld defects due to a lack of fusion were detected in some regions of specimens treated with a low-power laser region (3 J/mm), where a lack of fusion, in turn, occurred due to underheating. Bubble-shaped weld defects were observed in some specimens treated with a high-power laser region (5 J/mm); melting occurred due to the overheating of the specimen. Computer simulations were used to trace the boundaries of the fusion zone, and yielded results similar to the visual inspection ones. In the lower-energy region, the energy may not be sufficient to fuse the specimen, whereas the high-energy region may have sufficient energy to break down the polymer chains. A novel deep learning algorithm was used to statistically evaluate the weld quality. Approximately 1700–1900 samples were collected for each condition, and the pre-trained quality evaluation indicated a highly reliable (>98%) welding classification (fail or good). According to the results of this study, welding quality assessments based on visual inspection, computer simulation, and DL-based inspection yield similar results.
Development and Performance Evaluation of an Integrated Disc Cutter System for TBMs
Authors: Meng Zhichao , Yang Dongjian , Huo Junzhou , Zhuo Puzhou , Bao Youneng
Published: January 2021

Changing the cutter is an essential procedure in tunnel boring machine (TBM) operations. The danger and low efficiency of manually replacing the disc cutter has led engineering equipment designers to study technologies to automate the changing of cutters. The key step is to design a new integrated disc cutter (simplifying the replacement process) to facilitate robot operation. Until now, the design and research of new integrated disc cutter systems has mainly relied on inspiration. In this paper, a solution space of a certain configuration of an integrated disc cutter system based on topology theory was designed. The evaluation system for the solution space was constructed by the analytic hierarchy process (AHP), and the optimal scheme of the configuration was finally obtained. In addition, experiments and simulations show that the structural strength and anti-loosening performance of the new disc cutter system meet the engineering requirements. Application of topology theory and the new evaluation system provides useful theoretical analyses and guidance for the design of new cutter systems.
Modeling and Experimental Verification of Induction Heating of Thin Molybdenum Sheets
Authors: Frivaldsky Michal , Pavelek Miroslav , Donic Tibor
Published: January 2021

This paper deals with the issues relevant for precise finite element method (FEM) modeling of thin molybdenum plates’ induction heating. The proposed methodology describes the step-by-step Multiphysics (electro-thermal) design approach, verified by the experimental measurements. Initially, it was observed that the relative error between model and experimental set-up is within the 1.2% up to 2.5% depending on the location of the measuring points. Further research was focused on the enhancement of the simulation model in the form of its parametrization. It means that it is easy to define the induction coil’s operational parameters and geometrical properties (ferrite shape, operating frequency, the distance between plate and heating element, the value of coil current, etc.). The target of this approach is to be able to determine the optimal operational settings targeting the required heating performance of thin molybdenum plates. One of the main requirements regarding the optimal heating process is temperature distribution within the molybdenum plate’s surface. The proposed model makes it possible to obtain information on optimal operational conditions based on the received results.
Integrating Schedule Risk Analysis with Multi-Skilled Resource Scheduling to Improve Resource-Constrained Project Scheduling Problems
Authors: Isah Muritala Adebayo , Kim Byung-Soo
Published: January 2021

Construction projects are planned in a complex and dynamic environment characterized by high risks and uncertainties amidst resource constraints. Assessing construction schedule risk facilitates informed decision-making, especially in a resource-constrained situation, and allows proactive actions to be taken so that project objectives are not jeopardized. This study presents a stochastic multiskilled resource scheduling (SMSRS) model for resource-constrained project scheduling problems (RCSPSP) considering the impact of risk and uncertainty on activity durations. The SMSRS model was developed by integrating a schedule risk analysis (SRA) model (developed in MS Excel) with an existing multiskilled resource scheduling (MSRS) algorithm for the development of a feasible and realistic schedule. The computational experiment carried out on three case projects using the proposed SMSRS model revealed an average percentage deviation of 10.50%, indicating the inherent risk and uncertainty in activity durations of the project schedule. The core contribution of the proposed SMSRS model is that it: (1) presents project practitioners with a simple tool for assessing the risks and uncertainty associated with resource-constrained project schedules so that necessary response actions can be taken to ensure project success; (2) provides the small-scale construction businesses with an affordable tool for evaluating schedule risk and developing a feasible and realistic project schedule.