bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Oil Type on the Performance of β-Amyrin-Based Oleogels: Formation, Physicochemical Properties, and Potential Correlation Analysis
Authors: Su Shuxian , Qin Si , Xia Huiping , Li Peiwang , Li Haiyan , Li Chenjia , Guo Shiyin , Zeng Chaoxi
Published: March 2024

Pentacyclic triterpenes show potential as oleogelators, but their combination with various vegetable oils has limited research. This study selected linseed, rapeseed, sunflower, coconut, and palm oils to combine with the triterpenoid compound β-amyrin for the preparation of oleogels. The stability, crystal network structure, and other properties of each oleogel were evaluated. The correlation between different oil types and the properties of corresponding oleogels was explored. The results showed that β-amyrin formed stable oleogels with five vegetable oils under suitable temperature conditions, wherein especially the LO-based oleogel not only exhibited higher oil-binding capacity and hardness, but also demonstrated excellent stability at the microscopic level and notable rheological properties. Further analysis revealed a close correlation between the physicochemical properties of the oleogels and lipid characteristics, indicating that oleogels prepared from long-chain highly unsaturated fatty acids exhibit high stability. The above results indicate that β-amyrin can be a novel candidate oleogelator and that the oil type can modify the properties of β-amyrin-based oleogels. This study provides the latest reference for the application of pentacyclic triterpenoids in food.
Volatilomics-Based Discovery of Key Volatiles Affecting Flavor Quality in Tomato
Authors: Zhang Zhonghui , Ye Weizhen , Li Chun , Zhou Haihong , Wang Chao , Liu Penghui , Zhou Binxin , Zhao Hanqing , Wang Shouchuang , Yang Jun
Published: March 2024

Volatile accumulation during tomato ripening greatly affects the fruit flavor. In this study, four accessions from each of the three tomato subgroups (BIG, S. lycopersicum, CER, S. lycopersicumvar. Cerasiforme, and PIM, S. pimpinellifolium) were subjected to a sensory evaluation. The CER subgroup had the highest fruit-flavor score. Using a Headspace solid-phase microextraction/gas chromatography-mass spectrometer (HP-SPME/GC-MS), a volatile database containing 94 volatiles was created. Pentanal accumulated in green fruits and 1-pentanol in red fruits. 1-Octen-3-ol was discovered to underlie the bitterness of green tomatoes, and it was most abundant in PIM green fruits. Phenylethyl alcohol affected the acidity and sweetness of red tomatoes, and it was most abundant in CER red fruits. Branched-chain volatiles were most abundant in PIM and BIG red fruits, while apocarotenoids were most abundant in CER red fruits. These findings suggest that domestication and improvement have influenced volatile content, and apocarotenoids and branched-chain volatiles synergistically mediated aromatic flavors in red fruits. This study provides a metabolic basis for analyses of the molecular mechanisms of fruit-flavor formation.
Use of the Investigator ESSplex SE QS Kit (QIAGEN) at Half PCR Reaction Volumes for the Analysis of Forensic Samples
Authors: Barbaro Anna , Falcone Giacomo , La Marca Angelo , Barbaro Aldo
Published: March 2024

The Investigator ESSplex SE QS Kit (Qiagen) is a next-generation polymerase chain reaction (PCR) kit that, in 60 min, amplifies 17 Short Tandem Repeat (STR) markers, including the five European Standard Set (ESS) loci (D10S1248, D12S391, D1S1656, D22S1045, D2S441), the SE33 marker, and the locus Amelogenin for sex determination. Two quality sensors (QS1 and QS2) are also co-amplified to check PCR performance. Since forensic laboratories carry out hundreds of DNA typings annually, we verified the kit’s performance using half reaction volumes with the aim of improving the number of samples that may be amplified with a single kit and consequently reducing laboratory costs. In the present study, intended as a technical note rather than internal validation, some control samples (oral swabs) with known DNA profiles and 40 real casework samples were analyzed. We observed that reducing the total reaction volume, while keeping all component ratios unaltered, yields DNA profiles comparable to those obtained using standard reaction volumes and with allele peaks higher than those with regular volumes. Using half volumes for PCR amplification enables the analysis of a larger number of samples compared to the standard protocol, thereby reducing laboratory costs without compromising the quality of the analysis.
On a Faster Iterative Method for Solving Fractional Delay Differential Equations in Banach Spaces
Authors: Ugboh James Abah , Oboyi Joseph , Udo Mfon Okon , Nabwey Hossam A. , Ofem Austine Efut , Narain Ojen Kumar
Published: March 2024

In this paper, we consider a faster iterative method for approximating the fixed points of generalized α-nonexpansive mappings. We prove several weak and strong convergence theorems of the considered method in mild conditions within the control parameters. In order to validate our findings, we present some nontrivial examples of the considered mappings. Furthermore, we show that the class of mappings considered is more general than some nonexpansive-type mappings. Also, we show numerically that the method studied in our article is more efficient than several existing methods. Lastly, we use our main results to approximate the solution of a delay fractional differential equation in the Caputo sense. Our results generalize and improve many well-known existing results.
Biopsychosocial Predictors of Postpartum Depression: Protocol for Systematic Review and Meta-Analysis
Authors: Alhaj Ahmad Marwa , Al Awar Shamsa , Sayed Sallam Gehan , Alkaabi Meera , Smetanina Darya , Statsenko Yauhen , Zaręba Kornelia
Published: March 2024

During the postpartum period, psychological disorders may emerge. Aims and objectives: With the current study, we aim to explore the biological determinants that act on women during labor and incur the risk for postpartum depression (PPD). To reach the aim, we will perform the following tasks: (i) identify biological peripartum risk factors and calculate pooled prevalence of PPD for each of them; (ii) explore the strength of the relationship between peripartum risk factors and PPD; (iii) rank the predictors by their prevalence and magnitude of association with PPD. The knowledge obtained will support the development and implementation of early diagnostic and preventive strategies. Methods and analysis: We will systematically go through peer-reviewed publications available in the PubMed search engine and online databases: Scopus, Web of Science, EMBASE. The scope of the review will include articles published any time in English, Arabic, or Polish. We will deduplicate literature sources with the Covidence software, evaluate heterogeneity between the study results, and critically assess credibility of selected articles with the Joanna Briggs Institute’s bias evaluation tool. The information to extract is the incidence rate, prevalence, and odds ratio between each risk factor and PPD. A comprehensive analysis of the extracted data will allow us to achieve the objectives. The study findings will contribute to risk stratification and more effective management of PPD in women.
The Effects of Workload Excess on Quality of Work Life in Third-Level Healthcare Workers: A Structural Equation Modeling Perspective
Authors: Koca Mehmet , Deniz Serdar , İnceoğlu Feyza , Kılıç Ali
Published: March 2024

Examining the workload and working conditions of healthcare workers is crucial regarding the quality of the work life of employees and the quality of services provided. This study aims to determine the effects of the perceptions of workload on the quality of work life of health personnel working in two different tertiary hospitals affiliated with the Ministry of Health and the Higher Education Institution in Türkiye with structural equation modeling. This study was conducted in two hospitals in Malatya province: İnönü University Turgut Özal Medical Center affiliated with the Higher Education Institution and Malatya Training and Research Hospital affiliated with the Ministry of Health. The mean score of the Individual Workload Perception Scale was 106.18 ± 16.5, and the mean score of the Work-related Quality of Life Scale was 66.39 ± 15.33. Total workload scores had a statistically significant positive effect on total quality of work life scores (β1 = 0.98; p = 0.001). It was concluded that there was a statistically significant relationship between workload and quality of work life and age, unit of employment, working time in the profession, and hospital type.
Evidence-Based Recommendations in Primary Tracheoesophageal Puncture for Voice Prosthesis Rehabilitation
Authors: Mayo-Yáñez Miguel , Klein-Rodríguez Alejandro , López-Eiroa Aldán , Cabo-Varela Irma , Rivera-Rivera Raquel , Parente-Arias Pablo
Published: March 2024

Head and neck cancer, the seventh most common cancer worldwide, often affects the larynx, with a higher incidence in men. Total laryngectomy, a common treatment, results in the loss of phonation, and tracheoesophageal voice rehabilitation is the current rehabilitation method of choice. Despite ongoing debates regarding the timing of tracheoesophageal puncture (TEP), a crucial procedure for voice prosthesis placement, the secondary puncture continues to be the preferred choice in the majority of cases. This underscores the persistent controversy and the absence of consensus in this field. The aim of this manuscript was to define evidence-based recommendations regarding the procedure of primary TEP with voice prosthesis placement, establish the conditions and requirements for performing primary TEP, determine the indications and contraindications of primary TEP, as well as to define the complications and management of primary TEP. A total of 19 statements were formulated, with 78.95% of them having a Level of Evidence 4 and a Grade of Recommendation C. There is not sufficient evidence comparing the outcomes of primary TEP versus secondary TEP. Future studies with robust methodologies are needed to clarify the role of primary and secondary TEP in the rehabilitation of patients undergoing total laryngectomy.
ERK5 Interacts with Mitochondrial Glutaminase and Regulates Its Expression
Authors: Guillén-Pérez Yolanda María , Ortiz-Ruiz María Jesús , Márquez Javier , Pandiella Atanasio , Esparís-Ogando Azucena
Published: March 2024

Many of the biological processes of the cell, from its structure to signal transduction, involve protein–protein interactions. On this basis, our aim was to identify cellular proteins that interact with ERK5, a serine/threonine protein kinase with a key role in tumor genesis and progression and a promising therapeutic target in many tumor types. Using affinity chromatography, immunoprecipitation, and mass spectrometry techniques, we unveiled an interaction between ERK5 and the mitochondrial glutaminase GLS in pancreatic tumor cells. Subsequent co-immunoprecipitation and immunofluorescence studies supported this interaction in breast and lung tumor cells as well. Genetic approaches using RNA interference techniques and CRISPR/Cas9 technology demonstrated that the loss of ERK5 function led to increased protein levels of GLS isoforms (KGA/GAC) and a concomitant increase in their activity in tumor cells. It is well known that the tumor cell reprograms its intermediary metabolism to meet its increased metabolic needs. In this sense, mitochondrial GLS is involved in the first step of glutamine catabolism, one of the main energy sources in the context of cancer. Our data suggest that ERK5 contributes to the regulation of tumor cell energy metabolism via glutaminolysis.
Changes of Sublingual Microcirculation during the Treatment of Severe Diabetic Ketoacidosis
Authors: Krausova Vlasta , Neumann David , Skvor Jaroslav , Dostal Pavel
Published: March 2024

Introduction: Diabetic ketoacidosis (DKA) is associated with volume depletion and hemodynamic alterations. Changes in systemic microcirculation during DKA have not been described so far. Methods: In this case report, we describe the evolution of sublingual microcirculatory changes, monitored using sidestream dark field (SDF) imaging during the treatment of severe diabetic ketoacidosis in a 13-year-old girl. The patient presented a pH of 6.84, a glycemia level of 27.2 mmol/L, a ketonemia level of 5.6 mmol/L, a base excess of −29.4 mmol/L, hypernatremia, hyperosmolality due to acute gastritis, and a malfunction of the glucose sensor. Sublingual microcirculation measurements using an SDF probe were initiated 60 min after the initiation of treatment, which was then repeated 2, 3, 4, 6, 12, and 24 h after treatment initiation, as well as on the day of discharge. Results: Substantial alterations of microvascular perfusion parameters, both total and small vessel densities, perfused vessel densities, and the DeBacker score, were observed during the first 6 to 12 h of treatment. The degree of microcirculatory alteration was strongly negatively correlated with calculated osmolality, sodium levels, ketone and lactate levels, and blood pressure values. Conclusions: DKA is, in its complexity, associated with a serious microcirculatory alteration. SDF imaging provides insight into the severity of the patient’s microcirculatory alteration and its evolution during treatment.
Prevalence and Clinical Picture of Diamine Oxidase Gene Variants in Children and Adolescents with Attention Deficit Hyperactivity Disorder: A Pilot Study
Authors: Blasco-Fontecilla Hilario , Bella-Fernández Marcos , Wang Ping , Martin-Moratinos Marina , Li Chao
Published: March 2024

Background: Attention Deficit Hyperactivity Disorder (ADHD) is the most prevalent neurodevelopmental disorder worldwide. The diamine oxidase enzyme (DAO) is responsible for the histamine gastrointestinal degradation. Its deficient functioning may implicate an excess of histamine in the body. The excess of histamine (histamine intolerance, HIT) has been related with a growing number of diseases and pseudo-allergic symptomatology. However, data on the relationship between the DAO enzyme, HIT, and ADHD are lacking. The main objective of this pilot is to study the prevalence of the four most relevant SNP variants of the AOC1 gene affecting DAO enzyme functionality in a sample of patients diagnosed with ADHD attending child and adolescent mental health services. Methods: In a cohort of 303 participants, we measured the SNP variants of the AOC1 gene. Results: The prevalence of having at least one minor dysfunctional allele was 78.8%. No relationship between ADHD severity and DAO deficiency was found. However, some AOC1 gene variants associated with DAO deficiency were related to several meaningful medical comorbidities. Furthermore, we found a strong association between DAO activity and the intelligence quotient, particularly in working memory. Conclusions: Some SNP variants of the AOC1 gene associated with DAO deficiency are related to some medical comorbidities and cognitive dysfunction in ADHD children and adolescents. Studies including patients with other diagnoses and healthy controls and bigger samples are warranted to confirm our preliminary results.
Primary Thromboprophylaxis for the Prevention of Venous Thromboembolism in Cancer Patients with Central Venous Catheters: A Literature Review
Authors: Abdel-Razeq Hikmat , Al-Jaghbeer Mohammed J.
Published: March 2024

Cancer is a known risk factor for venous thromboembolism (VTE). The wider adoption of immunotherapy and anti-angiogenic drugs in recent years have increased this risk further. Central venous catheters (CVCs) are widely used access devices utilized to deliver infusion therapy, mostly in ambulatory settings. The endothelial injury associated with the use of these catheters adds to the risk of VTE to already high-risk patients. The introduction of direct oral anticoagulants (DOACs), with its proven efficacy and safety in multiple clinical indications, have renewed the attention to VTE prophylaxis in cancer patients with CVC. Several clinical trials and meta-analyses had shown that both apixaban and rivaroxaban are effective in lowering the risk of VTE, without increasing the risk of bleeding. Several risk assessment models (RAM) have utilized patient-related, tumor-related, and treatment-related factors, in addition to widely available biomarkers, like Hemoglobin (Hb) level, white blood cell (WBC) and platelets counts to stratify patients into two or three VTE risk levels. In this manuscript, we review the published clinical trials and meta-analyses that attempted to study the efficacy and safety of anticoagulants, mostly the DOACs, in cancer patients with CVCs. We will also propose a practical risk-directed approach to enhance VTE prophylaxis rate.
Innovative Wedge Anchorage for CFRP Plates: Development and Testing
Authors: Alhusain Mustafa , Al-Mayah Adil
Published: March 2024

Gripping prestressed carbon fiber-reinforced polymers (CFRPs) in structural strengthening applications is challenging due to CFRPs’ susceptibility to lateral loading. This paper presents a reliable and reusable wedge anchorage for gripping CFRP plates that are 50 mm wide and 1.2 mm thick. The cylindrical anchorage, which is 75 mm long and 76.2 mm in diameter, consists of an external steel barrel, two internal steel wedges, and two soft copper sleeves. The barrel-wedge interface is designed using an innovative arc–linear configuration, through which the desired stress distribution is attained, preventing stress concentration and the premature failure of the CFRP plate. The wedge anchorage was experimentally tested by applying a displacement-controlled tensile load of 0.6 mm/min until the complete fracture of the CFRP plate. The anchorage’s performance was examined under distinct installation conditions by applying different presetting levels: high (40–120 kN) and low (hammering) presetting. It was observed that the anchorage successfully prevented CFRP premature failure in all tests by achieving an average tensile loading of 172.3 (±5.7) kN, exceeding its reported tensile strength of 168 kN (2800 MPa). Minor CFRP displacements of 6.26 (±0.75) mm and 3.33 (±0.16) mm were recorded under low and high presetting levels, respectively. Similarly, the CFRP slippage relative to the wedges for the low and high presetting tests was only 1.18 (±0.75) mm and 0.33 (±0.15) mm, respectively. Also, only minor scratches were observed in the wedge–barrel interface, indicating the absence of extensive plastic deformation.
A Spatial Correlation Identification Model for Coherent Structure Extraction and Three-Dimensional Visualization
Authors: Mao Beibei , Yang Hua , Sun Fei , Zhang Ying , Zhang Xinrui
Published: March 2024

Multi-scale coherent structures have been observed in ocean currents, which are induced by the interaction of shear flows with different velocities. Understanding the spatial configuration and scale characteristics of coherent structures will promote the explanation of physical ocean phenomena. Considering the self-similarity, we propose a spatial correlation identification model for coherent structure extraction and three-dimensional visualization based on the wavelet transform and time-dependent intrinsic correlation method. The spatial and scale distributions of coherent structures are related to the dissipation rate variation. Most large-scale coherent structures, with the largest length scale of 13 m, are found to exist in stable fluid, such as the water column below 50 m. However, small-scale structures are found in chaotic fluids, such as the upper layer. Furthermore, we found that coherent structures of different scales coexist simultaneously in the same depth range, indicating a simultaneous multi-scale structure pattern for turbulent flow investigations.
Copper Sulfate Combined with Photodynamic Therapy Enhances Antifungal Effect by Downregulating AIF1
Authors: Zhang Meimei , An Qiyuan , Wang Yingzhe , Ye Shigan , Zhu Xiaoliang
Published: March 2024

Candida albicans is a clinically significant opportunistic fungus that is generally treated with antifungal drugs such as itraconazole and fluconazole. However, the recent emergence of fungal resistance has made treatment increasingly difficult. Therefore, novel antifungal treatment methods are urgently required. Hexanol ethosome photodynamic therapy (HE-PDT) is a method that uses photosensitizers (PS), such as hexanol ethosome, to exert antifungal effects, and can be used to treat resistant fungal strains. However, due to the high dose of PS required for antifungal treatment, excess photosensitizers may remain. Furthermore, once exposed to light, normal tissues or cells are damaged after photodynamic therapy, which limits the clinical application of HE-PDT. Therefore, improving the efficacy without increasing the dose is the key to this treatment. In this study, the antifungal effect of copper sulfate combined with HE-PDT was investigated, and its mechanism was explored. The results suggested that exogenous copper sulfate significantly increased the antifungal effect of HE-PDT by enhancing the rate of C. albicans inhibition, increasing reactive oxygen species (ROS) accumulation, increasing the rate of apoptosis, and altering the mitochondrial membrane potential (MMP) and ATP concentration, which is related to the downregulation of apoptosis-inducing factor (AIF1) expression. In conclusion, copper sulfate combined with photodynamic therapy significantly inhibited the activity of C. albicans by inducing apoptosis. The combined approach reported herein provides new insights for future antifungal therapy.
The Benefits of Workforce Well-Being on Profitability in Listed Companies: A Comparative Analysis between Europe and Mexico from an ESG Investor Perspective
Authors: De la Torre-Torres Oscar V. , Venegas-Martínez Francisco , Álvarez-García José
Published: March 2024

This paper evaluates the relationship between investing in workforce well-being and profitability of listed companies in Mexico compared to European companies from an Environmental, Social, and Governance (ESG) investor perspective. In this case, the Refinitiv workforce score or High-Performance Work Policies (HPWP) is used as an indicator of the quality of workforce well-being by including the industry effects (economic and business sectors) and the behavioral (sentiment) factors as control variables. Specifically, this article examines the relationships between HPWP, stock price changes (measured as a percentage), profitability (ROE), and market risk (betas). We used a sample of companies from the Refinitiv Mexico and European stock indices for this purpose. In the Mexican case, the results show that a higher level of well-being promotion relates to better company profits. The opposite happens in European companies. Regarding market prices, European companies show higher prices when they have higher HPWP and Mexican companies confirm the opposite. Regarding market risk, only European basic materials with high HPWP show less risk. Finally, in almost all Mexican business sectors, the relationship between market risk and workforce well-being is negative.
Evaluation and Driving Forces of Ecosystem Service Change in Maqu Alpine Wetland: An Emergy Approach
Authors: Han Ziyi , Zhao Ruifeng , Zhang Lihua , Chen Xidong , Wang Jingfa , Lu Haitian , Liu Fushou
Published: March 2024

Wetlands are among the most productive ecosystems on Earth, providing various key ecosystem services to humans and other organisms. However, an insufficient understanding of the economic value and importance of wetland resources has seriously weakened the ecosystem service value of wetlands. Thus, a more comprehensive understanding of wetland ecosystem services must be revealed in order to propose appropriate conservation policies. Therefore, to achieve the abovementioned goals, we used the emergy value accounting method to account for the ecosystem service value of the Maqu alpine wetlands, and the Logarithmic Mean Divisia Index was used to analyze the driving forces affecting changes in ecosystem services. The results indicate that the ecosystem service value of the Maqu alpine wetlands showed a trend of decline and then rebound during 1990–2020; its unit area emergy value decreased from 3.85 × 1010 sej/m2/y to 3.04 × 1010 sej/m2/y during 1990–2000, and after 2010, it began to gradually rebound. Moreover, the monetary ecosystem service value of the Maqu alpine wetlands continued to rise during the study period, reaching 41.45 USD/m2/y in 2020, thus reflecting the great potential value of wetland ecosystems. Based on our analysis, the key drivers affecting the changes in ecosystem services include economic scale and emergy benefit. Rapid and intensive human activities have boosted local economic development, but these developments have come at the cost of environmental degradation. Finally, some suggestions are proposed. This study suggests that the alpine wetlands in Maqu occupy an important ecological position within the whole Yellow River basin. Therefore, it is worthwhile to invest a significant amount of funding in their restoration and protection.
Extremophiles and Extreme Environments: A Decade of Progress and Challenges
Authors: Rampelotto Pabulo Henrique
Published: March 2024

It is with great enthusiasm that we embark on a retrospective journey to the landmark work “Extremophiles and Extreme Environments” (1 in Appendix A), published 10 years ago as part of its Special Issue (2 in Appendix A) [...]
Study of the Durability of Membrane Electrode Assemblies in Various Accelerated Stress Tests for Proton-Exchange Membrane Water Electrolysis
Authors: Su Zhengquan , Liu Jun , Li Pengfei , Liang Changhao
Published: March 2024

In this work, we focus on the degradation of membrane electrode assemblies (MEAs) in proton-exchange membrane water electrolysis (PEMWE) induced by different accelerated stress tests (ASTs), including constant-current mode, square-wave mode, and solar photovoltaic mode. In constant-current mode, at continuous testing for 600 h at 80 °C, a degradation of operating voltage increased by the enhanced current density from 22 µV/h (1 A/cm2) to 50 µV/h (3 A/cm2). In square-wave mode, we found that in the narrow fluctuation range (1–2 A/cm2), the shorter step time (2 s) generates a higher degradation rate of operating voltage, but in the wide fluctuation range (1–3 A/cm2), the longer step time (22 s) induces a faster operating voltage rise. In the solar photovoltaic mode, we used a simulation of 11 h sunshine duration containing multiple constant-current and square-wave modes, which is closest to the actual application environment. Over 1400 h ASTs, the solar photovoltaic mode lead to the most serious voltage rise of 87.7 µV/h. These results are beneficial to understanding the durability of the PEM electrolyzer and optimizing the components of MEAs, such as catalysts, membranes, and gas diffusion layers.
A Moiré Removal Method Based on Peak Filtering and Image Enhancement
Authors: Qi Wenfa , Yu Xinquan , Li Xiaolong , Kang Shuangyong
Published: March 2024

Screen photos often suffer from moiré patterns, which significantly affect their visual quality. Although many deep learning-based methods for removing moiré patterns have been proposed, they fail to recover images with complex textures and heavy moiré patterns. Here, we focus on text images with heavy moiré patterns and propose a new demoiré approach, incorporating frequency-domain peak filtering and spatial-domain visual quality enhancement. We find that the content of the text image mainly lies in the central region, whereas the moiré pattern lies in the peak region, in the frequency domain. Based on this observation, a peak-filtering algorithm and a central region recovery strategy are proposed to accurately locate and remove moiré patterns while preserving the text parts. In addition, to further remove the noisy background and paint the missing text parts, an image enhancement algorithm utilising the Otsu method is developed. Extensive experimental results show that the proposed method significantly removes severe moiré patterns from images with better visual quality and lower time cost compared to the state-of-the-art methods.
The Emission Reduction Technology Decision of the Port Supply Chain
Authors: Zhou Yan , Zhou Haiying
Published: March 2024

The technology options for sustainable development are explored with customer low-carbon preference in a port supply chain consisting of one ship and one port. Port supply chains can opt for either shower power or low-sulfur fuel oil to cut down emissions. We set game models considering three power structures: the port dominant (port-led Stackelberg game), the ship dominant (ship-led Stackelberg game), and the port and ship on the same footing (Nash game). We compare the performances of different technologies. It is shown that, when customer low-carbon preference and carbon tax are both low, LSFO is the appropriate choice from the supply chain’s profit perspective, SP is preferred from the emission control perspective, and LSFO is preferred from the social welfare perspective. However, when customers’ low-carbon preferences, carbon tax, and environmental concerns are all low or all high, LSFO should be adopted from the view of social welfare. The profits and carbon emissions of the supply chain in the Nash game are higher than those in the Stackelberg game. While the environmental concern is low, the social welfare of the supply chain in the Nash game is greater than that in the Stackelberg game. Otherwise, it is less than that in the Stackelberg game. The obtained results can help governments formulate policies and ships make emission reduction technology decisions according to their own interests.
Periodic Solutions to Nonlinear Second-Order Difference Equations with Two-Dimensional Kernel
Authors: Maroncelli Daniel
Published: March 2024

In this work, we provide conditions for the existence of periodic solutions to nonlinear, second-order difference equations of the form y(t+2)+by(t+1)+cy(t)=g(y(t)), where b and c are real parameters, c≠0, and g:R→R is continuous.
Exposure to Environmental Pesticides and the Risk of Autism Spectrum Disorders: A Population-Based Case-Control Study
Authors: Román Pablo , Ruiz-González Cristofer , Rueda-Ruzafa Lola , Cardona Diana , Requena Mar , Alarcón Raquel
Published: March 2024

Background and Objectives: Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by challenges in communication, social interactions, and repetitive behaviors. Although the factors that influence the development of this condition are unknown, certain chemical compounds such as pesticides have been proposed as possible contributors. Due to the lack of an established causal link between pesticide exposure and ASD, this study aimed to evaluate this potential association. Materials and Methods: A case-control study was carried out to ascertain the prevalence and risk associated with ASD in relation to pesticide exposure over a 21-year study period (2000–2021). Results: We included 2821 individuals diagnosed with ASD residing in areas of both high and low pesticide exposure in southern Spain. There was a rise in the ASD prevalence rate in regions with elevated pesticide use when compared to regions with low use [odds ratio (OR): 1.34, 95% confidence interval (CI), (1.24–1.44)]. Notably, men had the highest likelihood, with an OR: 1.42, 95% CI, (1.30–1.55). Furthermore, after performing multiple binary logistic regression adjusted for age, sex, and geographical area, males exhibited a higher likelihood compared to females [OR: 2.41, 95% CI, (2.21–2.62)]. Conclusions: Overall, this research suggests a connection between heightened environmental pesticide exposure due to increased agricultural use and autism.
Selective CO2 Capture from CO2/N2 Gas Mixtures Utilizing Tetrabutylammonium Fluoride Hydrates
Authors: Kim Hyeonjin , Ahn Yun-Ho
Published: March 2024

Gas hydrates, a type of inclusion compound capable of trapping gas molecules within a lattice structure composed of water molecules, are gaining attention as an environmentally benign gas storage or separation platform. In general, the formation of gas hydrates from water requires high-pressure and low-temperature conditions, resulting in significant energy consumption. In this study, tetrabutylammonium fluoride (TBAF) was utilized as a thermodynamic promoter forming a semi-clathrate-type hydrate, enabling gas capture or separation at room temperature. Those TBAF hydrate systems were explored to check their capability of CO2 separation from flue gas, the mixture of CO2 and N2 gases. The formation rates and gas storage capacities of TBAF hydrates were systematically investigated under various concentrations of CO2, and they presented selective CO2 capture behavior during the hydrate formation process. The maximum gas storage capacities were achieved at 2.36 and 2.38 mmol/mol for TBAF·29.7 H2O and TBAF·32.8 H2O hydrate, respectively, after the complete enclathration of the feed gas of CO2 (80%) + N2 (20%). This study provides sufficient data to support the feasibility of TBAF hydrate systems to be applied to CO2 separation from CO2/N2 gas mixtures based on their CO2 selectivity.
Exploring Deactivation Reasons of Biomass-Based Phosphorus-Doped Carbon as a Metal-Free Catalyst in the Catalytic Dehydroaromatization of n-Heptane
Authors: Yu Fei , Liu Siyuan , Liu Bo
Published: March 2024

Catalytic dehydroaromatization of n-alkanes into high-value aromatics has garnered extensive interest from both academia and industry. Our group has previously reported that phosphorus-doped carbon materials exhibit high selectivity for C-H bond activation in the dehydroaromatization of n-hexane. In this study, using n-heptane as a probe, we synthesized biomass-based phosphorus-doped carbon catalysts to investigate the impact of hydrogen heat treatment and carbon deposition on catalyst structure. Despite achieving an initial conversion of n-heptane at approximately 99.6%, with a toluene selectivity of 87.9%, the catalyst activity fell quickly. Moreover, longer hydrogen treatment time and higher hydrogen concentrations were found to accelerate catalyst deactivation. Thermogravimetric analysis (TGA) and N2 adsorption measurements (BET) indicated that a small amount of coke deposition was not the primary cause of catalyst deactivation. Temperature-programmed desorption of ammonia gas (NH3-TPD) revealed a significant decrease in acid-active functional groups. X-ray photoelectron spectroscopy (XPS) and solid-state 31P NMR spectroscopy confirmed the reduction of active central phosphorus species. These results suggest that catalyst deactivation primarily arises from the decrease in acidity and the partial reduction of phosphorus-containing groups, leading to a substantial loss of active sites. This work contributes new perspectives to understanding the properties and design improvements of metal-free carbon catalysts.
Refinement of the rKLi8.3-Based Serodiagnostic ELISA Allows Detection of Canine Leishmaniosis in Dogs with Low Antibody Titers
Authors: Teixeira Henrique C. , Valle Giulia P. C. , Mahdavi Rouzbeh , Dias Priscila S. M. , de Oliveira Erick E. , Aira Cristina P. , Heinz Daniela , Latz Andreas , Lana Marta de , Morgado Fernanda N. , Porrozzi Renato , Steinhoff Ulrich
Published: March 2024

The diagnosis of canine leishmaniasis (CanL) still represents a challenge due to the variable clinical manifestations and the large number of asymptomatic dogs. Serological tests are most commonly used to detect infected animals, revealing anti-Leishmania antibodies, mainly of the IgG isotype. Recently, a new diagnostic antigen, rKLi8.3, containing 8.3 kinesin tandem repeats (TR) from a Leishmania infantum strain from Sudan, has been shown to provide excellent specificity and sensitivity for the detection of Leishmania-infected humans and dogs. However, asymptomatic animals with very low antibody titers are often difficult to detect by serodiagnosis. Thus, we wondered whether the addition of an anti-IgG-enhancing step in the protein A/G-based rKLi8.3-ELISA will improve the diagnostic performance without decreasing the specificity. For this, parasitologically confirmed CanL cases with low or high clinical scores, uninfected healthy controls and dogs with other infections were tested by rKLi8.3-ELISA as well as two different immunochromatographic rapid tests, rKLi8.3-lateral flow test (LFT) and Dual Path Platform (DPP®) based on the rK28 antigen. Our results show that the diagnostic accuracies of the rKLi8.3-ELISA and LFT were similar to that of DPP, missing several asymptomatic animals. However, the addition of a secondary, amplifying anti-dog IgG antibody in the protein A/G-based rKLi8.3-ELISA enabled the detection of nearly all asymptomatic dogs without compromising its specificity.
The Effect of Heparin and Other Exogenous Glycosaminoglycans (GAGs) in Reducing IL-1β-Induced Pro-Inflammatory Cytokine IL-8 and IL-6 mRNA Expression and the Potential Role for Reducing Inflammation
Authors: Jafri Murtaza , Li Lin , Liang Binhua , Luo Ma
Published: March 2024

Glycosaminoglycans (GAGs) are long linear polysaccharides found in every mammalian tissue. Previously thought only to be involved in cellular structure or hydration, GAGs are now known to be involved in cell signaling and protein modulation in cellular adhesion, growth, proliferation, and anti-coagulation. In this study, we showed that GAGs have an inhibitory effect on the IL-1β-stimulated mRNA expression of IL-6 and IL-8. Exogenous heparin (p < 0.0001), heparan (p < 0.0001), chondroitin (p < 0.049), dermatan (p < 0.0027), and hyaluronan (p < 0.0005) significantly reduced the IL-1β-induced IL-8 mRNA expression in HeLa cells. Exogenous heparin (p < 0.0001), heparan (p < 0.0001), and dermatan (p < 0.0027) also significantly reduced IL-1β-induced IL-6 mRNA expression in HeLa cells, but exogenous chondroitin and hyaluronan had no significant effect. The exogenous GAGs may reduce the transcription of these inflammatory cytokines through binding to TILRR, a co-receptor of IL-1R1, and block/reduce the interactions of TILRR with IL-1R1.
Trimethyltin(IV) Bearing 3-(4-Methyl-2-oxoquinolin-1(2H)-yl)propanoate Causes Lipid Peroxidation-Mediated Autophagic Cell Death in Human Melanoma A375 Cells
Authors: Kasalović Marijana P. , Dimić Dušan , Jelača Sanja , Maksimović-Ivanić Danijela , Mijatović Sanja , Zmejkovski Bojana B. , Schreiner Simon H. F. , Rüffer Tobias , Pantelić Nebojša Đ. , Kaluđerović Goran N.
Published: March 2024

A novel trimethyltin(IV) complex (Me3SnL), derived from 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoate ligand, has been synthesized and characterized by elemental microanalysis, UV/Vis spectrophotometry, FT-IR and multinuclear (1H, 13C and 119Sn) NMR spectroscopies. Furthermore, the structure of the ligand precursor HL was solved using SC-XRD (single-crystal X-ray diffraction). The prediction of UV/Vis and NMR spectra by quantum-chemical methods was performed and compared to experimental findings. The protein binding affinity of Me3SnL towards BSA was determined by spectrofluorometric titration and subsequent molecular docking simulations. Me3SnL has been evaluated for its in vitro anticancer activity against three human cell lines, MCF-7 (breast adenocarcinoma), A375 (melanoma) and HCT116 (colorectal carcinoma), and three mouse tumor cell lines, 4T1 (breast carcinoma), B16 (melanoma) and CT26 (colon carcinoma), using MTT and CV assays. The strong inhibition of A375 cell proliferation, ROS/RNS upregulation and robust lipid peroxidation lead to autophagic cell death upon treatment with Me3SnL.
Experimental Demonstration to Enhance the Curvature Sensitivity of a Fiber Mach–Zehnder Interferometer Based on a Waist-Enlarged Technique Using Polymer
Authors: Martinez-Ramirez Laura G. , Hernández-Romano Iván , Guzmán-Cano Cipriano , Marrujo-García Sigifredo , Fernandez-Jaramillo Arturo A. , Estudillo-Ayala Julian M. , Rojas-Laguna Roberto , Sierra-Hernandez Juan M.
Published: March 2024

A fiber curvature sensor based on a Mach–Zehnder Interferometer (MZI) constructed using the waist-enlarged technique to splice a segment of non-zero dispersion-shifted fiber (NZ-DSF) between two segments of single mode fiber (SMF) is proposed and experimentally demonstrated. All fabricated sensors presented an improvement in their curvature sensitivity when they were coated with polydimethylsiloxane (PDMS) polymer. The sensor that exhibited the best performance was 6.5 cm long, with a curvature sensitivity of 8.27 nm/m−1 in a range of 0.69 m−1 (from 1.08 to 1.77 m−1). This sensitivity is 3.22 times higher than that of the sensor without polymer. Additionally, the sensor coated with polymer exhibited cross-sensitivity that is 2.23 times smaller than the sensor without polymer. The easy fabrication and notable performance of this device makes it alluring for structural health monitoring.
Determining Topological Charge of Bessel-Gaussian Beams Using Modified Mach-Zehnder Interferometer
Authors: Baliyan Mansi , Nishchal Naveen K.
Published: March 2024

The orbital angular momentum (OAM) associated with structured singular beams carries vital information crucial for studying various properties and applications of light. Determining OAM through the interference of light is an efficient method. The interferogram serves as a valuable tool for analyzing the wavefront of structured beams, especially identifying the order of singularity. In this study, we propose a modified Mach–Zehnder interferometer architecture to effectively determine the topological charge of Bessel–Gaussian (BG) beams. Several numerically generated self-referenced interferograms have been used for analysis. Moreover, this study examines the propagation property and phase distribution within BG beams after they are obstructed by an aperture in the interferometer setup.
Indirect Estimation of Heavy Metal Contamination in Rice Soil Using Spectral Techniques
Authors: Zhong Liang , Yang Shengjie , Rong Yicheng , Qian Jiawei , Zhou Lei , Li Jianlong , Sun Zhengguo
Published: March 2024

The rapid growth of industrialization and urbanization in China has led to an increase in soil heavy metal pollution, which poses a serious threat to ecosystem safety and human health. The advancement of spectral technology offers a way to rapidly and non-destructively monitor soil heavy metal content. In order to explore the potential of rice leaf spectra to indirectly estimate soil heavy metal content. We collected farmland soil samples and measured rice leaf spectra in Xushe Town, Yixing City, Jiangsu Province, China. In the laboratory, the heavy metals Cd and As were determined. In order to establish an estimation model between the pre-processed spectra and the soil heavy metals Cd and As content, a genetic algorithm (GA) was used to optimise the partial least squares regression (PLSR). The model’s accuracy was evaluated and the best estimation model was obtained. The results showed that spectral pre-processing techniques can extract hidden information from the spectra. The first-order derivative of absorbance was more effective in extracting spectral sensitive information from rice leaf spectra. The GA-PLSR model selects only about 10% of the bands and has better accuracy in spectral modeling than the PLSR model. The spectral reflectance of rice leaves has the capacity to estimate Cd content in the soil (relative percent difference [RPD] = 2.09) and a good capacity to estimate As content in the soil (RPD = 2.97). Therefore, the content of the heavy metals Cd and As in the soil can be estimated indirectly from the spectral data of rice leaves. This study provides a reference for future remote sensing monitoring of soil heavy metal pollution in farmland that is quantitative, dynamic, and non-destructive over a large area.
Study on Micro-Pressure Drive in the KKM Low-Permeability Reservoir
Authors: Zhang Heng , Wang Mibang , Ke Wenqi , Li Xiaolong , Yang Shengjun , Zhu Weihua
Published: March 2024

Kazakhstan has abundant resources of low-permeability oil reservoirs, among which the KKM low-permeability oil reservoir has geological reserves of 3844 × 104 t and a determined recoverable reserve of 1670 × 104 t. However, the water flooding efficiency is only 68%, and the recovery efficiency is as low as 32%. The development of the reservoir faces challenges such as water injection difficulties and low oil production from wells. In order to further improve the oil recovery rate of this reservoir, our team developed micro-pressure-driven development technology based on pressure-driven techniques by integrating theories of fluid mechanics and artificial intelligence. We also combined this with subsequent artificial lift schemes, resulting in a complete set of micro-pressure-driven process technology. The predicted results indicate that after implementing micro-pressure-driven techniques, a single well group in the KKM oilfield can achieve a daily oil production increase of 32.08 t, demonstrating a good development effect.
Modeling Surface Roughness and Flow of Gases in Threaded Connections to Analyze Sealing Performance
Authors: Zhu Wenqi , Liang Yu , Zhao Lv
Published: March 2024

Oil casings and premium threaded connections play vital roles in the oil and gas extraction industry. The present work establishes an integrated modeling framework for the sealability assessment of premium threaded connections which can be easily implemented and employed by engineers. The framework incorporates a part-scale finite element analysis of the make-up process, a micro-scale simulation of the contact behavior, and a mechanism-informed gap flow model. It is found that complete sealing can be achieved when the contact pressure exceeds 1540 MPa for Gaussian rough surfaces presenting a roughness of 1.6 μm. The seal surface fit is revealed to be critical for sealing performance, as it slightly changes the optimum make-up torque (up to 4%) but significantly changes contact pressure (up to 22%). At an optimum make-up torque, the connection with the loosest seal surface tolerance fit is prone to gas leakage when considering an inlet pressure of 110 MPa. The proposed modeling framework can be extended to other types of threaded connections with metal–metal contact sealing.
Influence of the Rotation Speed on the Internal Flow Characteristics of an Aircraft Fuel Gear Pump
Authors: Xu Boliang , Liu Qi , Zhu Zuchao , Gao Yongcao , Li Chuancang , Zhang Yuanding
Published: March 2024

A gear pump is a key rotary-displacement pump for aircraft fuel transportation in the aerospace industry. Due to the great ratio of power-to-weight condition demanded for gear pumps in aircraft fuel transportation systems, the parameter of the rotation speed is a matter of extreme concern affecting internal flow characteristics that determines the adverse effects of cavitation, fuel trapping, and vibration. However, the flow characteristics of an aircraft fuel gear pump influenced by the rotation speed have not been elaborated upon on yet. In this research, the flow characteristics of an aircraft fuel gear pump were studied by considering the influence of the rotation speed. An experiment for testing the external performance of an aircraft fuel gear pump was performed, and a corresponding numerical simulation of a gas–liquid two-phase flow was employed. Distributions of the velocity and pressure at the central cross-sections and their monitored transient developments were comparatively analyzed for different rotation speeds. It was found that a greater pressure oscillational amplitude accompanied by a higher frequency could be induced by a higher rotation speed, especially in the region of gear engagement. Additionally, cavitation evolution characteristics affected by the rotation speed in the fuel gear pump were discussed. The mechanism of cavitation generation in the region of gear engagement to withdrawal was revealed to be the quick release of a great amount of pressure. Furthermore, a dimensionless cavitation area was employed to quantify the periodic cavitation evolution, and the natural exponential development of the maximum dimensionless cavitation area with the rotation speed was determined through curve fitting. This study should be helpful for creating a deeper understanding of the internal flow characteristics of an aircraft fuel gear pump in scientific research and the external performance in aerospace industrial applications.