bluuebunny/crossref_metadata_2025 huggingface dataset:
Management of giant left atrial thrombus late after transcatheter mitral valve-in-ring replacement using a transcatheter aortic valve: a case report
Authors: Maloku A. , Hamadanchi A. , Bäz L. , Richter M. , Bargenda S. , Möbius-Winkler S. , Schulze P. C. , Franz Marcus
Published: November 2024

Background

In symptomatic high-risk patients with severe mitral valve regurgitation (MR), who are not eligible for surgery, Transcatheter edge-to-edge repair (TEER) or transcatheter mitral valve replacement (TMVR) may be an option, especially when surgical mitral valve repair by annuloplasty has been performed earlier. After TMVR, the appropriate anticoagulation regimen is still matter of debate.

Case presentation

We here report on a 78-year-old frail lady with heart failure and atrial fibrillation who underwent surgical reconstruction of the mitral valve nine years ago. Due to high surgical risk and after heart team discussion, TMVR using a transcatheter aortic valve prosthesis (valve-in-ring concept) was performed successfully via the transapical access route. Several months later, an excellent result could be confirmed. Since surgical excision of the left atrial appendage was carried out during first surgery, oral anticoagulation was withdrawn. Two months later, the patient presented with a massive LA thrombus mass and severe stenosis of the mitral valve prosthesis requiring re-do surgery.

Conclusions

Management of anticoagulation in patients with atrial fibrillation and successfully performed LAA excision is still a matter of debate, in particular after transcatheter heart valve implantation in mitral position. TMVR devices may be very thrombogenic. Thus, caution should be used whenever discontinuing oral anticoagulation in these patients. Despite the lack of evidence, withdrawal of anticoagulation should be avoided here, especially in the absence of bleeding complications. Left atrial appendage closure or excision should not influence this decision.
Isolation and identification of antifungal, antibacterial and nematocide agents from marine bacillus gottheilii MSB1
Authors: Shehata Ahmed. S. , Samy Marwa A. , Sobhy Sherien E. , Farag Aida M. , El-Sherbiny Ibrahim M. , Saleh Ahmed A. , Hafez Elsayed E. , Abdel-Mogib Mamdouh , Aboul-Ela Haiam M.
Published: November 2024

Pathogenic fungi employ numerous strategies to colonize plants, infect them, reduce crop yield and quality, and cause significant losses in agricultural production. The increasing use of chemical pesticides has led to various ecological and environmental issues, including the emergence of resistant weeds, soil compaction, and water pollution, all negatively impacting agricultural sustainability. Additionally, the extensive development of synthetic fungicides has adverse effects on animal and human health, prompting the exploration of alternative approaches and green strategies for phytopathogen control. Microorganisms living in sponges represent a promising source of novel bioactive secondary metabolites, potentially useful in developing new nematicidal and antimicrobial agents. This study focuses on extracting bioactive compounds from endosymbiotic bacteria associated with the marine sponge Hyrtios erect sp. (collected from NIOF Station, Hurghada, Red Sea, Egypt) using various organic solvents. Bacillus sp. was isolated and identified through 16 S rRNA gene sequencing. The biocidal activity of Bacillus gotheilii MSB1 extracts was screened against plant pathogenic bacteria, fungi, and nematodes. The n-butanol extract showed significant potential as a biological fungicide against Alternaria alternata and Fusarium oxysporum. Both n-hexane and ethyl acetate extracts exhibited negative impacts against the plant pathogenic bacteria Erwinia carotovora and Ralstonia solanacearum, whereas the n-butanol extract had a positive effect. Regarding nematicidal activity, ethyl acetate and n-butanol extracts demonstrated in-vitro activity against the root-knot nematode Meloidogyne incognita, which causes serious vegetable crop diseases, but the n-hexane extract showed no positive effects. The findings suggest that bioactive compounds from endosymbiotic bacteria associated with marine sponges, particularly B. gotheilii MSB1, hold significant potential as alternative biological control agents against plant pathogens. The n-butanol extract, in particular, displayed promising biocidal activities against various plant pathogenic fungi, bacteria, and nematodes. These results support further exploration and development of such bioactive compounds as sustainable, environmentally friendly alternatives to synthetic pesticides and fungicides in agricultural practices.
Adapting and pilot testing a tool to assess the accessibility of primary health facilities for people with disabilities in Luuka District, Uganda
Authors: Mactaggart Islay , Ssemata Andrew Sentoogo , Menya Abdmagidu , Smythe Tracey , Rotenberg Sara , Marks Sarah , Bannink Mbazzi Femke , Kuper Hannah
Published: November 2024

Background

People with disabilities frequently experience barriers in seeking healthcare that lead to poorer health outcomes compared to people without disabilities. To overcome this, it is important to assess the accessibility of primary health facilities – broadly defined to include a disability-inclusive service provision – so as to document present status and identify areas for improvement. We aimed to identify, adapt and pilot test an appropriate tool to assess the accessibility of primary health facilities in Luuka District, Uganda.

Methods

We conducted a rapid literature review to identify appropriate tools, selecting the Disability Awareness Checklist (DAC) on account of its relative brevity and development as a sensitization and action tool. We undertook three rounds of adaptation, working together with youth researchers (aged 18–35) with disabilities who then underwent 2 days of training as DAC facilitators. The adapted tool comprised 71 indicators across four domains and 12 sub-domains. We also developed a structured feedback form for facilitators to complete with healthcare workers. We calculated median accessibility scores overall, per domain and per sub-domain, and categorised feedback form suggestions by type and presumed investment level. We pilot-tested the adapted tool in 5 primary health facilities in one sub-district of Luuka, nested within a pilot healthcare worker training on disability.

Results

The median overall facility accessibility score was 17.8% (range 12.3–28.8). Facility scores were highest in the universal design and accessibility domain (25.8%, 22.6–41.9), followed by reasonable accommodation (20.0%, 6.7–33.3). Median scores for capacity of facility staff (6.67%, 6.7–20.0), and linkages to other services were lower (0.0%, 0–25.0). Within the feedback forms, there were a median of 21 suggestions (range 14–26) per facility. Most commonly, these were categorised as minor structural changes (20% of suggestions), with a third categorised as no (2%) or low (33%) cost, and the majority (40%) medium cost.

Conclusions

Overall accessibility scores were low, with many opportunities for low-cost improvement at the facility level. We did not identify any issues with the implementation of the tool, suggesting few further adaptations are required for its future use in this setting.
Predicting steroid-induced osteonecrosis of the femoral head: role of lipid metabolism biomarkers and radiomics in young and middle-aged adults
Authors: Jia Daqi , Zhang Yue , Li Huaqiang , Guo Chunfang , Wu Yipeng , Shi Xiangwen , Yang Li , Mo Jieyu , Liu Xia , Xu Yongqing
Published: November 2024

Background

Femoral head necrosis is a common orthopedic disease that results in significant physical disability in patients. Early prediction and diagnosis of steroid-induced osteonecrosis of the femoral head (SONFH) are crucial for the prevention and treatment of this condition.

Methods

In this study, initial CT images and clinical data of patients with SONFH, admitted from January 2019 to December 2022, were collected. Patients were grouped as follows: (1) those diagnosed with SONFH at the initial diagnosis (control group), and (2) those with high-risk factors but no symptoms at first diagnosis, who developed SONFH two years later (experimental group). CT imaging histological features, clinical characteristics, and transcriptome screening for differentially expressed genes, pathway enrichment, and immune infiltration analyses were performed.

Results

Significant differences were found in triglyceride (TG) levels between the training and validation groups. Age, sex, alkaline phosphatase (ALP), and hemoglobin levels differed between the training and internal validation groups, while HDL and red blood cell counts varied between the training and external validation groups. Univariate analysis showed that age, TG, HDL, and Radiomics scores influenced SONFH, while multivariate analysis revealed TG, HDL, and Radiomics scores were closely related to SONFH. Transcriptomic analysis showed associations with sphingolipid and adipocyte signaling pathways, along with immune cell involvement, linking SONFH to lipid metabolism and atherosclerosis.

Conclusions

These findings indicate a significant association between steroid-induced osteonecrosis of the femoral head and age, with TG and HDL serving as indicators of lipid metabolism closely correlated with the occurrence of SONFH. Radiomics scores were also found to correlate with SONFH occurrence, supported by transcriptomic and CT imaging findings. However, this study has limitations, including its retrospective design and a relatively limited sample size, which may impact the generalizability of the results. Further prospective studies with larger, more diverse populations are needed to validate and enhance the predictive model.
Bacteriocin Microcin J25’s antibacterial infection effects and novel non-microbial regulatory mechanisms: differential regulation of dopaminergic receptors
Authors: Shang Lijun , Yang Fengjuan , Chen Qingyun , Dai Ziqi , Yang Guangxin , Zeng Xiangfang , Qiao Shiyan , Yu Haitao
Published: November 2024

Background

The antibacterial and immunomodulatory activities of bacteriocins make them attractive targets for development as anti-infective drugs. Although the importance of the enteric nervous system (ENS) in the struggle against infections of the intestine has been demonstrated, whether it is involved in bacteriocins anti-infective mechanisms is poorly defined.

Results

Here, we demonstrated that the bacteriocin Microcin J25 (J25) significantly alleviated diarrhea and intestinal inflammation in piglets caused by enterotoxigenic Escherichia coli (ETEC) infection. Mechanistically, macrophage levels were significantly downregulated after J25 treatment, and this was replicated in a mouse model. Omics analysis and validation screening revealed that J25 treatment induced significant changes in the dopaminergic neuron pathway, but little change in microbial structure. The alleviation of inflammation may occur by down-regulating dopamine receptor (DR) D1 and the downstream DAG-PKC pathway, thus inhibiting arachidonic acid decomposition, and the inhibition of macrophages may occur through the up-regulation of DRD5 and the downstream cAMP-PKA pathway, thus inhibiting NF-κB.

Conclusions

Our studies’ findings provide insight into the changes and possible roles of the ENS in J25 treatment of ETEC infection, providing a more sophisticated foundational understanding for developing the application potential of J25.
Fallacy of paleoproductivity signals by the recycled biogenic components: case study in the Central Basin of the northwestern Ross Sea
Authors: Khim Boo-Keun , Kim Sunghan , Lee Min Kyung , Sohn Young Kwan , Lee Jae Il , Yoo Kyu-Cheul
Published: November 2024

Core LC42, retrieved from the Central Basin of the northwestern Ross Sea, contains three distinct sediment facies (IRD (ice-rafted debris)-poor bioturbated sandy mud, IRD-rich massive sandy mud, and laminated mud) that are interleaved with each other and deposited over the last 1 Ma. The biogenic components (biogenic opal, total organic carbon, and total nitrogen) of the laminated mud layers are consistently higher than the other two facies throughout the core. Based on the depositional succession of sediment facies and IRD-related depositional processes in the Antarctic continental margin, the laminated mud layers without IRD have been deposited during the glacial periods, but the enhanced paleoproductivity in terms of biogenic components during the mud deposition is unexpected. Backscattered electron imagery substantiates distinctly different componentry of the alternating light and dark laminae in the laminated mud. In particular, the light laminae contain scattered diatom fragments and eroded sand-sized lumps of fossil-bearing mud, whereas the dark laminae are clayey and diatomaceous. Both laminae thus are characterized by the high biogenic components, but they are interpreted to have been principally recycled from older deposits because diatom fragments are mostly reworked and old and their archives are poorly preserved. During the glacial periods, these laminated muds were deposited downward by milky plumes of meltwater discharged underneath the advancing glaciers that scoured the earlier-deposited and semi-consolidated diatom-rich sediments. Our study thus highlights that the recycling of biogenic particles should be precautious to avoid the fallacy of paleoclimatic interpretation in formulating climate-productivity models in the Antarctic continental margin.
What shapes statistical and data literacy research in K-12 STEM education? A systematic review of metrics and instructional strategies
Authors: Friedrich Anja , Schreiter Saskia , Vogel Markus , Becker-Genschow Sebastian , Brünken Roland , Kuhn Jochen , Lehmann Jessica , Malone Sarah
Published: November 2024

The pervasive digitization of society underscores the crucial role of data and its significant impact on decision-making across various domains. As a result, it is essential for individuals to acquire competencies in handling data. This need is particularly pertinent in K-12 education, where early engagement with data and statistics can lay a foundational understanding for future academic and professional endeavors. Additionally, K-12 education should provide students with critical skills necessary for navigating the complexities of daily life and making informed decisions in a data-rich society. This systematic review examines the state of research on statistical and data literacy in K-12 STEM (Science, Technology, Engineering, and Mathematics) education. It focuses specifically on cognitive, affective, and behavioral metrics and pedagogical approaches empirically investigated in this context. Using a rigorous selection process, we identified and synthesized 83 original empirical papers. Additionally, we invited the authors of these studies to share their perspectives on future strategies for addressing statistical and data literacy. The results indicate that the included studies primarily focus on the construct of statistical literacy, which is operationalized through a diverse array of metrics, predominantly within the context of mathematics education. We identified effective pedagogical approaches, such as authentic problem-solving and the integration of real-world data. The researchers surveyed emphasized the importance of interdisciplinary teaching, adapted curricula, and improved professional development for pre- and in-service teachers. Our findings underscore the growing relevance of this field, but suggest that integrated perspectives on statistical and data literacy within STEM subjects are limited.
In vitro antifungal activities of medicinal plants used for treatment of candidiasis in Pader district, Northern Uganda
Authors: Akwongo Betty , Kakudidi Esezah K. , Nsubuga Anthony M. , Andama Morgan , Namaganda Mary , Tugume Patience , Asiimwe Savina , Anywar Godwin , Katuura Esther
Published: November 2024

Background

The emergence of multidrug resistant Candida species to available drugs has led to renewed interest in the use of herbal medicines globally. This study scientifically verified antifungal effectiveness of five commonly used plant species in Pader district, against selected pathogenic candida strains.

Methods

Powdered roots of Momordica foetida, Sansevieria dawei and Distimake dissectus; and stem barks of Khaya anthotheca and Mitragyna rubrostipulata were extracted sequentially using petroleum ether and methanol, respectively; and total water extraction at 24.4 °C (maceration), 60 °C (decoction) and boiling water at 87 °C (hot water infusion). Extracts and their combinations, positive controls (amphotericin B, and fluconazole) and negative control (80% dimethyl sulfoxide, verified to be tolerable concentration to the tested Candida species) were screened and verified for their antifungal activity against Candida albicans (ATCC: American Type Culture Collection reference strain 10231, ATCC 90028, 0770a and 0796), C. glabrata (VVc 004, ATCC 2950) and C. tropicalis (ATCC 750 and 0210) using agar well diffusion and broth micro-dilution, respectively.

Results

Aqueous extract (24.4 °C) of M. rubrostipulata (ZOI: 18.00 ± 1.00 to 38.33 ± 0.17; MIC: 3.13 ± 0.00 to 20.83 ± 4.17; MFC: 12.50 ± 0.00 to 200.00 ± 0.00), methanol extract of K. anthotheca (10.11 ± 0.31 to 15.11 ± 0.65; 1.04 ± 0.26 to 12.50 ± 0.00; 12.50 ± 0.00 to 100.00 ± 0.00), and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca (7.89 ± 0.26 to 19.67 ± 0.37; 0.78 ± 0.00 to 50.00 ± 0.00; 12.50 ± 0.00 to 200.00 ± 0.00) exhibited broad spectrum antifungal activities and were fungistatic against all tested Candida species, which comprised 8 clinical/control and susceptible/resistant strains. None of the conventional drugs used demonstrated broad spectrum antifungal activity across all tested Candida species/strains.

Conclusion

Methanol extract of K. anthotheca, aqueous extract (24.4 °C) of M. rubrostipulata, and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca could be effective in the treatment of candidiasis. They demonstrated potential broad spectrum antifungal activity against different species and strains of tested Candida than the fluconazole and amphotericin B drugs. Their fungistatic nature showed their ability to inhibit fungal growth. Hence, these extracts/extract combination can offer better treatment option for candidiasis if they are standardized and also their active curative compounds isolated and made into antifungal drugs.
Prevalence of potential drug‒drug interactions and associated factors among elderly patients in Ethiopia: a systematic review and meta-analysis
Authors: Alemayehu Tekletsadik Tekleslassie , Wassie Yilkal Abebaw , Bekalu Abaynesh Fentahun , Tegegne Addisu Afrassa , Ayenew Wondim , Tadesse Gebresilassie , Getachew Demis , Yazie Abebaw Setegn , Teketelew Bisrat Birke , Mekete Mekonnen Derese , Fentahun Setegn , Abebe Tesfaye Birhanu , Minwagaw Tefera , Geremew Gebremariam Wulie
Published: November 2024

Background

The occurrence of potential drug‒drug interactions (pDDIs) is a serious global issue that affects all age groups, with the elderly population being the most vulnerable. This is due to their relatively high rates of comorbidity and polypharmacy, as well as physiological changes that can increase the potential for DDIs and the likelihood of adverse drug reactions. The aim of this study was to estimate the prevalence of pDDIs and associated factors among elderly patients in Ethiopia.

Methods

A comprehensive literature search using the preferred reporting items for systematic review and meta-analysis statement was conducted on HINARI, Science Direct, Embase, PubMed/MEDLINE, Google Scholar, and Research Gate. Data were extracted via a Microsoft Excel spreadsheet and analyzed via STATA version 11.0. Egger regression tests and funnel plot analysis were used to check publication bias, and the I2 statistic was used to evaluate statistical heterogeneity. Sensitivity and subgroup analyses were also conducted to identify potential causes of heterogeneity.

Results

Seven articles were analyzed, and a total of 1897 pDDIs were identified in 970 patients, resulting in an average of 1.97 DDIs per patient. The number of DDIs per patient ranged from 0.18 to 5.86. The overall prevalence of pDDIs among elderly patients was 50.69% (95% CI 18.77–82.63%). However, the prevalence of pDDIs ranged widely from 2.80 to 90.1%. When the severity of the interactions was considered, the prevalence of potential DDIs was found to be 28.74%, 70.68%, and 34.20% for major, moderate, and minor pDDIs, respectively. Polypharmacy and long hospital stays were identified as factors associated with pDDIs among elderly patients in Ethiopia.

Conclusions

The overall prevalence of pDDIs among elderly patients was high, with a wide range of prevalence rates. Moderate-severity interactions were the most prevalent. Polypharmacy and long hospital stays were identified as factors associated with pDDIs among elderly patients. The study suggests that DDIs identification database itself could have modified the DDIs prevalence rate. As a result, a single DDIs identification database needs to be authorized; otherwise, clinical knowledge should be taken into account when interpreting the information obtained.
Treatment with tofacitinib attenuates muscle loss through myogenin activation in the collagen-induced arthritis
Authors: da Rosa Thales Hein , Bartikoski Bárbara Jonson , do Espírito Santo Rafaela Cavalheiro , Farinon Mirian , de Souza Silva Jordana Miranda , Pedó Renata Ternus , Gasparini Maria Luísa , Karnopp Thais , dos Santos Leonardo Peterson , Chapacais Gustavo , di Domenico Andressa , Loch Sofia , Xavier Ricardo Machado
Published: November 2024

Background

Sarcopenia is a muscle disease characterized by reduction of muscle strength and muscle mass. In RA, 25.9 to 43.3% of the patients present sarcopenia. The loss of muscle mass observed in RA patients occurs either by activation of catabolic pathways or by inhibition of anabolic pathways. Despite having a list of drugs capable of treating RA inflammation, their effect on muscle is unclear. Our objective was to evaluate the tofacitinib effect on the muscle mass of collagen-induced arthritis (CIA) mice.

Methods

CIA was induced in male DBA/1J mice by subcutaneous injection of Type 2 Collagen plus Freund Adjuvant. Animals were randomized into 3 groups: CIA + tofacitinib; CIA + vehicle; and healthy controls. Treatment was administered twice a day, between days 18 and 45 after induction. Clinical score, edema, and body weight were evaluated during the experimental period. After euthanasia, tibiotarsal joints were collected for assessment of disease histopathological score, and tibialis anterior (TA) and gastrocnemius (GA) muscles were weighed to assess muscle mass. Muscle atrophy was evaluated by measurement of TA myofiber cross-sectional area (CSA). Protein expression was evaluated by western blot using GA homogenates. Serum inflammatory markers were evaluated by ELISA. Statistical analysis included ANOVA followed by Tukey’s or with Kruskal-Wallis. The statistical difference was assumed for p < 0.05.

Results

Tofacitinib treatment decreased arthritis severity by reducing clinical score, and hind paw edema in comparison with the vehicle group. Tofacitinib showed weight gain, higher TA and GA weights, and increased CSA compared to the vehicle group. On day 45, Tofacitinib presented increased muscle strength compared to the vehicle group, however, no difference was found in muscle fatigue. Pax7 expression was unchanged, while MyoD expression showed an increasing trend, and myogenin expression was significantly increased in Tofacitinib compared to vehicle and control groups. The treatment didn’t modify Murf-1 expression. Tofacitinib mice showed decreased serum levels of TNF and increased IL-6 serum levels.

Conclusion

Tofacitinib attenuated muscle loss in arthritic mice, increased muscle weight and muscle CSA. Activation of satellite cell regeneration, based on the increased expression of myogenin, is a potential mechanism involved in tofacitinib action against muscle loss.
QTc interval prolongation impact on in-hospital mortality in acute coronary syndromes patients using artificial intelligence and machine learning
Authors: El Amrawy Ahmed Mahmoud , Abd El Salam Samar Fakhr El Deen , Ayad Sherif Wagdy , Sobhy Mohamed Ahmed , Awad Aya Mohamed
Published: November 2024

Background

Prediction of mortality in hospitalized patients is a crucial and important problem. Several severity scoring systems over the past few decades and machine learning models for mortality prediction have been developed to predict in-hospital mortality. Our aim in this study was to apply machine learning (ML) algorithms using QTc interval to predict in-hospital mortality in ACS patients and compare them to the validated conventional risk scores.

Results

This study was retrospective, using supervised learning, and data mining. Out of a cohort of 500 patients admitted to a tertiary care hospital from September 2018 to August 2020, who presented with ACS. Prediction models for in-hospital mortality in ACS patients were developed using 3 ML algorithms. We employed the ensemble learning random forest (RF) model, the Naive Bayes (NB) model and the rule-based projective adaptive resonance theory (PART) model. These models were compared to one another and to two conventional validated risk scores; the Global Registry of Acute Coronary Events (GRACE) risk score and Thrombolysis in Myocardial Infarction (TIMI) risk score. Out of the 500 patients included in our study, 164 (32.8%) patients presented with unstable angina, 148 (29.6%) patients with non-ST-elevation myocardial infarction (NSTEMI) and 188 (37.6%) patients were having ST-elevation myocardial infarction (STEMI). 64 (12.8%) patients died in-hospital and the rest survived. Performance of prediction models was measured in an area under the receiver operating characteristic curve (AUC) ranged from 0.83 to 0.93 using all available variables compared to the GRACE score (0.9 SD 0.05) and the TIMI score (0.75 SD 0.02). Using QTc as a stand-alone variable yielded (0.67 SD 0.02) with a cutoff value 450 using Bazett’s formula, whereas using QTc in addition to other variables of personal and clinical data and other ECG variables, the result was 0.8 SD 0.04. Results of RF and NB models were almost the same, but PART model yielded the least results. There was no significant difference of AUC values after replacing the missing values and applying class balancer.

Conclusions

The proposed method can effectively predict patients at high risk of in-hospital mortality early in the setting of ACS using only clinical and ECG data. Prolonged QTc interval can be used as a risk predictor of in-hospital mortality in ACS patients.
Performance of complementor’s complementary goods on platform ecosystems: the perspective of individual complementors' complementary goods development behavior
Authors: Zhou Xiaoxiao , Inoue Yuki
Published: November 2024

The purpose of our study was to examine how individual complementors' development behaviors (specifically, diversity- and distinctiveness-oriented development behaviors) impact the performance of complementary goods on the platform. To achieve this, we employed fixed-effects regression analysis and utilized panel data obtained from the free music platform “DOVA.” This study examines two aspects of the performance of complementor’s complementary goods: the highest performance related to the success of the platform ecosystem and the lowest performance related to platform ecosystem sustainment. The findings indicate that a high focus on diversity-oriented development behavior is positively correlated with the complementor's highest performance and negatively correlated with complementor's lowest performance. Additionally, distinctiveness-oriented development behavior also positively correlated with complementor's highest performance. However, low distinctiveness-oriented development behavior is negatively correlated with complementor's lowest performance. We reveal desirable development behaviors for complementary goods related to both content innovation and platform ecosystem sustainability. Based on these understandings, platform owners can target platform governance.
Physiotherapists’ knowledge, attitude and practice of clinical prediction rules in low-back pain
Authors: Mbada Chidozie Emmanuel , Afolabi Victor Adeboye , Oghumu Saturday N. , Gebrye Tadesse , Ademoyegun Adekola , Adje Mishael , Karstens Sven , Fatoye Francis
Published: November 2024

Background and aim

Clinical prediction rules (CPRs) are mathematical tools that are intended to guide clinicians in clinical decision making or predict a future outcome, but they seem rather unknown, under-utilized, or avoided by clinicians. This study aimed to assess knowledge, attitude, and practice of CPRs in low-back pain (LBP) among physiotherapists.

Methods

A cross-sectional study involving 45 consenting specialist musculoskeletal physiotherapists from three public-funded teaching hospitals in Nigeria was carried out. An adapted validated questionnaire on facilitators and barriers to CPRs utilization, and a socio-demographic proforma were used to collect data. Descriptive and inferential statistics were employed to analyze data. Alpha level was set at p < 0.05.

Results

Respondents were mostly males (71.1%), married (64.4%) and first-degree holders (55.6%). Twenty-eight (62.2%) of the respondents had above-average knowledge of CPRs in LBP. Rates for positive attitude towards, and utilization of CPRs were 37.8% and 15.6%. Knowledge and attitude about CPRs in LBP were not significantly influenced by socio-demographic factors (p > 0.05). However, there was a significant association between the utilization of CPRs and years of experience (χ2 = 10.339 p = 0.016).

Conclusion

Most Nigerian physiotherapists had above-average knowledge, but a negative attitude and low utilization of CPRs in LBP. Clinicians’ years of clinical experience influence the usage of CPR. There is a need to incorporate training in CPRs into undergraduate and continuous professional development programmes.
XAF1 antagonizes TRIM28 activity through the assembly of a ZNF313-mediated destruction complex to suppress tumor malignancy
Authors: Jang Seung-Hun , Choi Hwi-Wan , Ahn Jieun , Jang Sungchan , Yoon Ji-Hye , Lee Min-Goo , Chi Sung-Gil
Published: November 2024

X-linked inhibitor of apoptosis-associated factor 1 (XAF1) is a stress-inducible pro-apoptotic protein that is commonly inactivated in multiple human cancers. Nevertheless, the molecular basis for its tumor suppression function remains largely uncharacterized. Here we report that XAF1 antagonizes the oncogenic activity of tripartite motif containing 28 (TRIM28) ubiquitin E3 ligase through zinc finger protein 313 (ZNF313)-induced ubiquitination and proteasomal degradation. XAF1 exerts apoptosis-promoting effect more strongly in TRIM28+/+ versus XAF1−/− tumor cells and suppresses tumor cell growth, migration, invasion, and epithelial-to-mesenchymal transition and xenograft tumor growth in a highly TRIM28-dependent fashion. Mechanistically, XAF1 interacts directly with the RING domains of TRIM28 and ZNF313 through the ZF6 and ZF7 domain, respectively, thereby facilitating ZNF313 interaction with and ubiquitination of TRIM28. A mutant XAF1 lacking either ZF6 or ZF7 domain exhibits no activity to promote TRIM28 ubiquitination. By destabilizing TRIM28, XAF1 blocks TRIM28-driven ubiquitination of p53 and RLIM, p53-HDAC1 interaction, and TWIST1 stabilization. Intriguingly, TRIM28 destabilizes XAF1 through K48-linked polyubiquitination and proteasomal degradation to protect tumor cells from apoptotic stress, indicating its role as an intrinsic antagonist against XAF1 and the antagonistic interplay of XAF1 and TRIM28. XAF1 expression is inversely correlated with TRIM28 expression in cancer cell lines and tumor tissues and more tightly associated with the survival of TRIM28-high versus TRIM28-low patients. Together, this study uncovers a novel mechanism by which XAF1 suppresses tumor malignancy and an important role for XAF1-TRIM28 interplay in governing stress response, illuminating the mechanistic consequence of its alteration during tumorigenic process.
Insights on the structural framework of the Egyptian Eastern Desert derived from edge detectors of gravity data
Authors: Saada Saada A. , Eleraki M. , Mansour Aya , Eldosouky Ahmed M.
Published: November 2024

Advanced geophysical techniques are used on Bouguer gravity data to unravel the structural complexity of the Eastern Desert (ED). Using methods such as tilt angle (TA), enhanced horizontal gradient amplitude (EHGA), lineament analysis, and 2D crustal modeling, we are able to differentiate between deep and shallow gravity sources and delineate the area’s geologic features. Using fast Fourier transform, positive and negative anomalies are identified. The positive anomalies are interpreted as uplifted block, anticline features, or intrusive occurrences of denser rocks, whereas the negative anomalies are interpreted as downfaulted blocks, providing crucial insights. Lineament analysis suggests that the predominant tectonic force that shaped the area is in the northwest direction. Integration of TA and EHGA reveals nuanced structural patterns. The constructed structural map indicates a network of uplifted and downfaulted blocks intersected by strike-slip faults with a northeast trend. The 2D crustal modeling illustrates the diverse composition of granitic and basaltic rocks, with a discernible thinning of the continental crust toward the Red Sea offshore area. This significantly advances the understanding of the ED’s geologic evolution, offering valuable insights into the tectonic processes and crustal dynamics.
Automatic conditioning of marine seismic angle stack data with a convolutional neural network
Authors: Huff Owen Rohwer , Evensen Andreas Kjelsrud , Lie Jan Erik , Bugge Aina Juell , Sablon Ronan , Thorkildsen Vemund Stenbekk , Faleide Jan Inge
Published: November 2024

Misalignment of reflections is a common problem in migrated prestack seismic data, which occurs due to moveout correction with incomplete knowledge of the velocity model. This issue persists in partial angle stack data often used for amplitude-variation-with offset (AVO) analyses and is traditionally treated with residual moveout (RMO) and trim static corrections. However, these methods require time-consuming velocity selection and parameter tuning and commonly lead to spurious alignment of reflections with noise. Therefore, we train a convolutional neural network (CNN) on synthetic examples to automatically perform conditioning and alignment of angle stack data. First, 2D synthetic common-depth point gathers are created using a convolutional modeling method and then made into input-target pairs, where the inputs are poorly moveout corrected (using inaccurate normal-moveout velocities) and the targets are accurately moveout corrected. These input-target examples are split into angle stacks for the near, mid, and far offsets and then used to train a CNN to transform the misaligned angle stacks into their more-aligned counterparts. In testing, the trained network increases the alignment of unseen synthetic data, improving the correlation with the target far-offset trace from 0.76 to 0.85 on average. The network is applied on two field examples from offshore Norway with class 3 and class 2P AVO responses, respectively. In both cases, the angle stack data predicted by the network indicate improved alignment, greater resolution in the far-offset stacked section, and increased correspondence to a well-tie synthetic seismogram, all while retaining the AVO responses. The CNN predictions are compared with angle stack data after traditional conditioning (RMO and trim static corrections), with the results being of similar or greater quality. This method enables high-quality conditioning of angle stack data at a fraction of the time required for conventional methods and is easily adaptable to different data sets.
Trunk climbing among ground-dwelling European amphibians – first observation from Poland
Authors: Kaczmarski Mikołaj , Kubicka Anna Maria
Published: November 2024

Most European amphibian species are considered land-dwelling, associated with moving along the ground and as poor climbers. However, more recent research indicates that non-arboreal amphibians use nesting boxes and tree hollows in Central and Western Europe. This study describes the first observation of tree climbing by the common toad Bufo bufo (Linnaeus, 1758) and common frog Rana temporaria Linnaeus, 1758 in Poland. While conducting a night survey of newt mortality in the ‘Traszki Ratajskie’ protected area in an urban park in the city of Poznań, Poland, we detected the unexpected use of trees by two species of amphibians. In June 2020, we observed cases of Bufo bufo and Rana temporaria foraging on and using arboreal ambush sites. The toads were found at an average height of 124 cm above the ground, and the frogs at 35 cm, on the tree trunks and branches of two willows (Salix cfr. alba L.) and a dead silver birch specimen (Betula pendula L.). The willows had an extensive multi-stem structure, and exhibited signs of maturity and ageing (hollow-bearing trees with open cavities and crevices). This is the first observation of arboreal behaviour in B. bufo and R. temporaria in Poland. The climbing behaviour and use of tree sites were likely linked to the search for a humid shelter provided by the interior of the ageing trees, as well as to the foraging behaviour that we observed. Trees exhibiting signs of maturity usually have complex structures, and seem particularly important for amphibians during periods of drought, as both a suitable shelter and an area from which to ambush prey. We recommend focusing greater attention on the protection of mature, ageing trees, especially Salix spp. within a terrestrial buffer zone of at least 250 metres from ponds, as these are poorly researched components within amphibian habitats. This type of resource may be essential for long-term amphibian conservation due to site fidelity and the limited number of shelters in human-altered landscapes. The recently adopted EU Nature Restoration Law presents an opportunity to develop good practices in this area, particularly urban tree planting and habitat management.
Notes on the phally polymorphism in Vertigo lilljeborgi (Westerlund, 1871), V. geyeri Lindholm, 1925, V. genesii (Gredler, 1856) and V. ronnebyensis (Westerlund, 1871) in Sweden
Authors: von Proschwitz Ted , Książkiewicz Zofia
Published: November 2024

In this paper we present some new information about the phally polymorphism of four Vertigo species: Vertigo lilljeborgi, V. geyeri, V. genesii and V. ronnebyensis. Material for examinations was collected from 7 sites in Sweden in 2021 and 2023. Despite a small number of individuals examined, all three sexual morphs (aphally, euphally, and hemiphally) were found in V. geyeri and V. genesii. Hemiphallics were recorded in these two species for the first time. In V. lilljeborgi we only found aphallics and euphallics, while in V. ronnebyensis only aphallics were found.
Clinical practice guidelines for the diagnosis and treatment of
scabies in Korea: Part 1. Epidemiology, clinical manifestations, and diagnosis
— a secondary publication
Authors: Park Jin , Kwon Soon-Hyo , Lee Young Bok , Kim Hei Sung , Jeon Jie Hyun , Choi Gwang Seong
Published: November 2024

Scabies is a skin disease caused by the parasite Sarcoptes
scabiei var. hominis, which is primarily
transmitted via direct skin or sexual contact or, less commonly, via contact
with infested fomites. In Korea, the incidence of scabies has decreased from
approximately 50,000 cases per year in 2010 to about 30,000 cases per year in
2021. However, outbreaks are consistently observed in residential facilities,
such as nursing homes, especially among older adults. The clinical
manifestations of scabies vary based on the patient’s age, health status,
the number of mites, and the route of transmission. Typical symptoms of classic
scabies include intense nocturnal itching and characteristic skin rashes
(burrows and erythematous papules), with a predilection for the interdigital web
spaces, inner wrists, periumbilical areas, axillae, and genital areas. In
contrast, older adults with immunodeficiency or neurological disorders may
exhibit hyperkeratotic scaly lesions or an atypical distribution with mild to no
itching (crusted scabies). The diagnosis of scabies is based on clinical
symptoms and the results of diagnostic tests aimed at identifying the presence
of the parasite. While a history of close contact and characteristic clinical
findings suggest scabies, confirmation of the diagnosis requires detecting
scabies mites, eggs, or scybala. This can be achieved through light microscopy
of skin samples, non-invasive dermoscopy, and other high-resolution in
vivo imaging techniques.
A Note on Gödel’s First Disjunct Formalised in DTK System
Authors: Corradini Antonella , Galvan Sergio
Published: November 2024

This note clarifies the significance of the proof of Gödel’s first disjunct obtained through the formalisation of Penrose’s second argument within the DTK system. It analyses two formulations of the first disjunct – one general and the other restricted – and dwells on the demonstration of the restricted version, showing that it yields the following result: if by F we denote the set of propositions derivable from any formalism and by K the set of mathematical propositions humanly knowable, then, given certain conditions, F necessarily differs from K. Thus it is possible that K surpasses F but also, on the contrary, that F surpasses K. In the latter case, however, the consistency of F is humanly undecidable.
Literal and Controllable Paraconsistency
Authors: Ciuciura Janusz
Published: November 2024

The principle of explosion asserts that any formula can be derived from any pair of other contradictory formulas. Paraconsistent logic is typically regarded as a logic in which the universal validity of this principle is questioned. Therefore, a key point is determining when the validity can be considered universal to classify a logic as paraconsistent. A pertinent example to illustrate this point is the calculus CB1 that admits the principle but only for negated formulas, i.e., from any set {α, ∼α} any other formula follows if and only if α is of the form ∼γ. Another example is Sette’s calculus P1, which is paraconsistent at the level of variables but not complex formulas. Both serve as compelling examples of the so-called borderline cases.
In this paper, we examine several calculi expected to be paraconsistent at the level of literals. It means that a pair of formulas, α and ∼α, can yield any β if, and only if α is neither a propositional variable nor is its iterated negation. Furthermore, it is assumed that in some calculi presented here, β must adhere to specific restrictions. Once these conditions are satisfied, we refer to calculus as paraconsistent in a “controllable manner”.
La geografía y la guerra, los geógrafos y la paz: ampliando las agendas políticas y de investigación
Authors: Mamadouh Virginie
Published: November 2024

La relación estrecha entre la disciplina de la geografía y la violencia militar en proyectos tanto nacionalistas como imperialistas está bien establecida. Sin embargo, hasta hace poco, la guerra rara vez ha sido un tema prominente en las investigaciones geográficas. Aún menos se ha tratado la paz como objeto de estudio, a pesar de que siempre ha habido geógrafos que han intentado usar la geografía para promover la paz o deslegitimar la guerra y la  violencia. Este trabajo ofrece una visión de las numerosas agendas políticas y de investigación que se han  desarrollado recientemente en la geografía en relación con la guerra y la paz
Del orden de los saberes al orden de los libros a través de la memoria de una biblioteca mercedaria novohispana (1755)
Authors: Benítez Palacios Claudia Alejandra , Guzmán Guzmán Yolanda
Published: November 2024

El orden de los libros entendido no como el acomodo físico en una estantería, sino como la clasificación temática de los libros que conformaban una biblioteca, institucional o privada, según el orden de los saberes o facultades en el que se estructuraba el conocimiento en la Edad Moderna, quedó plasmado en la colección de impresos y manuscritos de las bibliotecas formadas en el Virreinato de la Nueva España. El objetivo de este capítulo es indagar el orden de los libros que estaba presente en las bibliotecas según diversos documentos históricos, como inventarios y catálogos,1 para comprender de qué manera se articulaban los saberes durante el periodo virreinal. Para adentrarnos en este análisis, se tomará como punto de partida la Memoria de los libros que tiene esta librería del convento de la Inmaculada Concepción, Recolección de Ntra Señora de la Merced de las Huertas, cuio libro se hizo en veinte días del mes de abril de mil setecientos sinquenta y sinco resguardado por la Biblioteca Nacional de México (BNM), con la signatura MS10252.
Navigating the digital frontier: transforming endodontic diagnosis through digitization. part 1
Authors: D'Souza Janina Loren , Mala Kundabala , Grover Srishti , Singh Ankita
Published: November 2024

The current advances and innovations in material technology and armamentaria help to negate the difficulties faced by clinicians in diagnosing and treating patients. Oral healthcare contributes much to the wellbeing of a person. However, the oral cavity is a small and difficult area in which to work since it is filled with vascularized mobile tissues and various shaped teeth, and connected to sinuses, nerve canals, lungs and the gastrointestinal tract via the trachea and pharynx. Moreover, each tooth has a complex root canal system that must be explored if it is infected or inflamed. Root canal treatment can be compared to entering a blind tunnel and treating something unseen. Endodontics is a field in dentistry where a clinician must use their tactile sense and correlate it with clinical knowledge, as well as skill, to treat dental infections. Digital evolution has simplified diagnosis, treatment planning, documentation and communication with patients, rendering more predictable outcomes and providing evidence for the healing or non-healing of disease. The evolution of digital radiography has offered us the best technology available. The present review discusses various helpful digital tools that are currently available, and future technologies that may guide clinicians in better diagnosis.

CPD/Clinical Relevance:

Advances in diagnostic tools have improved accuracy in identifying and treating dental pathologies, thereby achieving more predictable outcomes and enhancing patient care.