bluuebunny/crossref_metadata_2025 huggingface dataset:
Substrate Specificity of an Aminopropyltransferase and the Biosynthesis Pathway of Polyamines in the Hyperthermophilic Crenarchaeon Pyrobaculum calidifontis
Authors: Fukuda Wakao , Osaki Mamoru , Yasuda Yusuke , Hidese Ryota , Higuchi Tsunehiko , Umezawa Naoki , Fujiwara Shinsuke , Mizohata Eiichi
Published: May 2022

The facultative anaerobic hyperthermophilic crenarchaeon Pyrobaculum calidifontis possesses norspermine (333), norspermidine (33), and spermidine (34) as intracellular polyamines (where the number in parentheses represents the number of methylene CH2 chain units between NH2, or NH). In this study, the polyamine biosynthesis pathway of P. calidifontis was predicted on the basis of the enzymatic properties and crystal structures of an aminopropyltransferase from P. calidifontis (Pc-SpeE). Pc-SpeE shared 75% amino acid identity with the thermospermine synthase from Pyrobaculum aerophilum, and recombinant Pc-SpeE could synthesize both thermospermine (334) and spermine (343) from spermidine and decarboxylated S-adenosyl methionine (dcSAM). Recombinant Pc-SpeE showed high enzymatic activity when aminopropylagmatine and norspermidine were used as substrates. By comparison, Pc-SpeE showed low affinity toward putrescine, and putrescine was not stably bound in its active site. Norspermidine was produced from thermospermine by oxidative degradation using a cell-free extract of P. calidifontis, whereas 1,3-diaminopropane (3) formation was not detected. These results suggest that thermospermine was mainly produced from arginine via agmatine, aminopropylagmatine, and spermidine. Norspermidine was produced from thermospermine by an unknown polyamine oxidase/dehydrogenase followed by norspermine formation by Pc-SpeE.
Redox Control of Signalling Responses to Contractile Activity and Ageing in Skeletal Muscle
Authors: Jackson Malcolm J. , Pollock Natalie , Staunton Caroline , Jones Samantha , McArdle Anne
Published: May 2022

Research over almost 40 years has established that reactive oxygen species are generated at different sites in skeletal muscle and that the generation of these species is increased by various forms of exercise. Initially, this was thought to be potentially deleterious to skeletal muscle and other tissues, but more recent data have identified key roles of these species in muscle adaptations to exercise. The aim of this review is to summarise our current understanding of these redox signalling roles of reactive oxygen species in mediating responses of muscle to contractile activity, with a particular focus on the effects of ageing on these processes. In addition, we provide evidence that disruption of the redox status of muscle mitochondria resulting from age-associated denervation of muscle fibres may be an important factor leading to an attenuation of some muscle responses to contractile activity, and we speculate on potential mechanisms involved.
Exercise Enhances Branched-Chain Amino Acid Catabolism and Decreases Cardiac Vulnerability to Myocardial Ischemic Injury
Authors: Wu Guiling , Guo Yanjie , Li Min , Li Chenhan , Tan Yanzhen , Li Yueyang , Li Jia , Wang Li , Zhang Xing , Gao Feng
Published: May 2022

Long-term exercise-induced metabolic adaptations occupy a central position in exercise-afforded cardiac benefits. Emerging evidence suggests that branched-chain amino acid (BCAA) catabolic defect contributes to cardiac dysfunction in multiple cardiometabolic diseases. However, the role of BCAA catabolism in exercise-afforded cardiac benefits remains unknown. Here, we show that exercise improves BCAA catabolism and thus reduce cardiac vulnerability to myocardial ischemic injury. Exercise increased circulating BCAA levels in both humans (male adolescent athletes) and mice (following an 8-week swimming intervention). It increased the expression of mitochondrial localized 2C-type serine-threonine protein phosphatase (PP2Cm), a key enzyme in regulating BCAA catabolism, and decreased BCAA accumulation in mouse hearts, indicating an increase in BCAA catabolism. Pharmacological promotion of BCAA catabolism protected the mouse heart against myocardial infarction (MI) induced by permanent ligation of the left descending coronary artery. Although cardiac-specific PP2Cm knockout showed no significant effects on cardiac structural and functional adaptations to exercise, it blunted the cardioprotective effects of exercise against MI. Mechanistically, exercise alleviated BCAA accumulation and subsequently inactivated the mammalian target of rapamycin in MI hearts. These results showed that exercise elevated BCAA catabolism and protected the heart against myocardial ischemic injury, reinforcing the role of exercise in the promotion of cardiac health.
Chronic Pediatric Pain in Low- and Middle-Income Countries
Authors: Walters Camila B. , Kynes J. Matthew , Sobey Jenna , Chimhundu-Sithole Tsitsi , McQueen K. A. Kelly
Published: May 2022

Chronic pain is a serious health concern and potentially debilitating condition, leading to anxiety, depression, reduced productivity and functionality, and poor quality of life. This condition can be even more detrimental and incapacitating in the pediatric patient population. In low- and middle-income countries (LMICs), pain services are often inadequate or unavailable, leaving most of the world’s pediatric population with chronic pain untreated. Many of these children in LMICs are suffering without treatment, and often die in pain. Awareness and advocacy for this population must be prioritized. We reviewed the available literature on the chronic pediatric pain burden in LMICs, barriers to treatments, and current efforts to treat these patients.
Modeling of Imperfect Viscoelastic Interfaces in Composite Materials
Authors: Cruz-González Oscar , Rodríguez-Ramos Reinaldo , Lebon Frederic , Sabina Federico
Published: May 2022

The present work deals with hierarchical composites in three dimensions, whose constituents behave as non-aging linear viscoelastic materials. We model the influence that imperfect viscoelastic interfaces have on the macroscopic effective response of these structures. As an initial approach, the problem of two bodies in adhesion is studied and in particular the case of soft viscoelastic interface at zero-order is considered. We deduce the integral form of the viscoelastic interface by applying the matched asymptotic expansion method, the correspondence principle, and the Laplace–Carson transform. Then, by adapting the integral form previously obtained, we address a heterogeneous problem for periodic structures. Here, theoretical results obtained for perfect interfaces are extended to the formal viscoelastic counterpart of the spring-type imperfect interface model. Finally, we show the potential of the proposed approach by performing calculations of effective properties in heterogeneous structures with two- and three-scale geometrical configurations and imperfect viscoelastic interfaces.
Van der Waals Epitaxial Growth of ZnO Films on Mica Substrates in Low-Temperature Aqueous Solution
Authors: Chen Hou-Guang , Shih Yung-Hui , Wang Huei-Sen , Jian Sheng-Rui , Yang Tzu-Yi , Chuang Shu-Chien
Published: May 2022

In this article, we demonstrate the van der Waals (vdW) epitaxial growth of ZnO layers on mica substrates through a low-temperature hydrothermal process. The thermal pretreatment of mica substrates prior to the hydrothermal growth of ZnO is essential for growing ZnO crystals in epitaxy with the mica substrates. The addition of sodium citrate into the growth solution significantly promotes the growth of ZnO crystallites in a lateral direction to achieve fully coalesced, continuous ZnO epitaxial layers. As confirmed through transmission electron microscopy, the epitaxial paradigm of the ZnO layer on the mica substrate was regarded as an incommensurate van der Waals epitaxy. Furthermore, through the association of the Mist-CVD process, the high-density and uniform distribution of ZnO seeds preferentially occurred on mica substrates, leading to greatly improving the epitaxial qualities of the hydrothermally grown ZnO layers and obtaining flat surface morphologies. The electrical and optoelectrical properties of the vdW epitaxial ZnO layer grown on mica substrates were comparable with those grown on sapphire substrates through conventional solution-based epitaxy techniques.
Evaluation of the Characteristics of Cholesteric Liquid Crystal Diffuser Element Applied in Multi-Focal Display Architectures
Authors: Ozols Ainars , Linina Elza , Zabels Roberts , Komitov Lachezar
Published: May 2022

Solid-state multi-focal and volumetric technologies highlight the future of 3D-display development. One of the most convenient implementations of multi-focal 3D displays are stacks of transparent liquid crystal displays. In this work, the core element is dissected—a switching optical diffuser element based on cholesteric liquid crystals, playing the role of a transparent display. In the present study, high-speed synchronized optical spectroscopy is used. We analyzed the kinetic and electro-optical characteristics of the diffuser element, the operation of which is based on the switching between diffuse and transparent states of this element. The underlying aim of this study was to investigate ways to improve some of these characteristics. It has been found that the transient peak in the optical transmission during field-off state, which is reducing the intensity of the light scattered by the diffuser element, is likely not associated to the assumed formation of the transient planar state. As the origin of this peak, we suggest a transient state possessing uniform lying helix structure, formed due the material flow taking place in the cell during relaxation of the liquid crystal. The role of the contacting surface’s pre-tilt angle in the switching process of the liquid crystal diffuser was established.
Facile Two-Step Deposition of Calcium Oxalate Film on Dolomite to Improve Acid Rain Resistance
Authors: Zha Jianrui , Gu Yaoqi , Wei Shuya , Han Huarui , Wang Feng , Ma Qinglin
Published: May 2022

The deposition of a calcium oxalate layer on dolomite demonstrates potential application in stone culture heritage conservation. However, due to insufficient coverage and the presence of cracks, the film’s usefulness is restricted. In this investigation, we used a simple two-step procedure to create a cohesive and uncracked film. The findings show that the protective layer provides better coverage of the dolomite surface without causing cracks and significantly improves acid resistance. Furthermore, after the simple two-step treatment, the color and adhesive strength of dolomite substrates remained nearly unchanged.
High-Pressure Polymorphism in Hydrogen-Bonded Crystals: A Concise Review
Authors: Yan Tingting , Xi Dongyang , Fang Qiuxue , Zhang Ye , Wang Junhai , Wang Xiaodan
Published: May 2022

High-pressure polymorphism is a developing interdisciplinary field. Pressure up to 20 GPa is a powerful thermodynamic parameter for the study and fabrication of hydrogen-bonded polymorphic systems. This review describes how pressure can be used to explore polymorphism and surveys the reports on examples of compounds that our group has studied at high pressures. Such studies have provided insight into the nature of structure–property relationships, which will enable crystal engineering to design crystals with desired architectures through hydrogen-bonded networks. Experimental methods are also briefly surveyed, along with two methods that have proven to be very helpful in the analysis of high-pressure polymorphs, namely, the ab initio pseudopotential plane–wave density functional method and using Hirshfeld surfaces to construct a graphical overview of intermolecular interactions.
Ultrastructure Traits and Genetic Variability of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) Adults from Different Geographical Locations in Egypt
Authors: El-Zoghby Islam R. M. , Awad Nabil S. , Alkhaibari Abeer Mousa , Abdel-Hameid Naglaa F.
Published: May 2022

The Red Palm Weevil (RPW) is one of the most damaging pests to palm cultivation; this invasive weevil poses a threat to the palm industry. The characterization and identification of this pest in order to determine its biological diversity is the first step in controlling it, which will help in developing effective control programs. The purpose of this study is to investigate the biodiversity of and characterize RPW from five different Egyptian geographical locations at morphological and genetic levels using morphometric analysis, scanning electronic microscopy and two different genetic markers. Our results revealed no significant differences between length and width of the adult body among RPW adults from different geographical locations. Different typologies of prothoracic spots were observed, indicating a degree of diversity in the RPW populations. The magnitude of the different body parts was measured among both males and females. Significant differences were exhibited between length of the antennal seta, as well as forelegs, the lengths and widths of the pronotum, and the rostrum length between both sexes. Both RAPD and ISSR used DNA markers, generating reproducible and distinct banding patterns. The polymorphic banding patterns that have resulted from all studied populations confirmed that these markers demonstrate genetic variability amongst the studied Egyptian populations of Rhynchophorus ferrugineus. The recorded differences may be due to the presence of different red palm weevil genotypes. The obtained results might have potential applications in developing a new tracking and control strategy for this invasive pest.
Nested Stochastic Valuation of Large Variable Annuity Portfolios: Monte Carlo Simulation and Synthetic Datasets
Authors: Gan Guojun , Valdez Emiliano A.
Published: May 2022

Dynamic hedging has been adopted by many insurance companies to mitigate the financial risks associated with variable annuity guarantees. To simulate the performance of dynamic hedging for variable annuity products, insurance companies rely on nested stochastic projections, which is highly computationally intensive and often prohibitive for large variable annuity portfolios. Metamodeling techniques have recently been proposed to address the computational issues. However, it is difficult for researchers to obtain real datasets from insurance companies to test metamodeling techniques and publish the results in academic journals. In this paper, we create synthetic datasets that can be used for the purpose of addressing the computational issues associated with the nested stochastic valuation of large variable annuity portfolios. The runtime used to create these synthetic datasets would be about three years if a single CPU were used. These datasets are readily available to researchers and practitioners so that they can focus on testing metamodeling techniques.
Spongiotic Pattern in Pemphigus: A Retrospective Observational Single-Center Study
Authors: Preclaro Ivan Arni C. , Wu Yu-Hung
Published: May 2022

Pemphigus is a chronic blistering disorder caused by autoantibodies that target desmosomal proteins in the epidermis. Acantholysis may be absent, and pemphigus may present only with spongiosis and vesiculation, thereby leading to a misdiagnosis of eczema. Herein, we conducted a retrospective, observational, single-center study to establish a pattern of spongiosis in cases of pemphigus confirmed by direct immunofluorescence. Immunopathologically diagnosed pemphigus specimens from 2001 to 2020 were retrieved, and specimens with spongiosis were analyzed for the following features: vesiculation, acantholysis, spongiosis, inflammatory cells in the epidermis, and inflammation in the dermis. Cases of spongiotic dermatitis were used as control. Out of 99 immunopathologically diagnosed pemphigus specimens, 41 samples with spongiosis were identified. About one quarter of the specimens did not have acantholysis. Spongiosis in the middle to lower thirds of the perilesional epidermis (p = 0.030), exocytosis with either neutrophils or eosinophils (p = 0.016), dermal infiltrates composed of lymphocytes, eosinophils, and neutrophils (p = 0.012), and absence of Langerhans cell microabscesses (p < 0.001) were more common in pemphigus than control. Spongiosis in pemphigus may mimic eczema in patients without acantholysis. The subtle histological findings in this study provide diagnostic clues and suggest that further immunofluorescence should be performed to confirm pemphigus diagnosis.
Correction: Sibanda et al. Application of Drone Technologies in Surface Water Resources Monitoring and Assessment: A Systematic Review of Progress, Challenges, and Opportunities in the Global South. Drones 2021, 5, 84
Authors: Sibanda Mbulisi , Mutanga Onisimo , Chimonyo Vimbayi G. P. , Clulow Alistair D. , Shoko Cletah , Mazvimavi Dominic , Dube Timothy , Mabhaudhi Tafadzwanashe
Published: May 2022

In the original publication [...]
Assessing Time Series Reversibility through Permutation Patterns
Authors: Zanin Massimiliano , Rodríguez-González Alejandro , Menasalvas Ruiz Ernestina , Papo David
Published: May 2022

Time irreversibility, i.e., the lack of invariance of the statistical properties of a system under time reversal, is a fundamental property of all systems operating out of equilibrium. Time reversal symmetry is associated with important statistical and physical properties and is related to the predictability of the system generating the time series. Over the past fifteen years, various methods to quantify time irreversibility in time series have been proposed, but these can be computationally expensive. Here, we propose a new method, based on permutation entropy, which is essentially parameter-free, temporally local, yields straightforward statistical tests, and has fast convergence properties. We apply this method to the study of financial time series, showing that stocks and indices present a rich irreversibility dynamics. We illustrate the comparative methodological advantages of our method with respect to a recently proposed method based on visibility graphs, and discuss the implications of our results for financial data analysis and interpretation.
Prescribed-Time Convergent Adaptive ZNN for Time-Varying Matrix Inversion under Harmonic Noise
Authors: Liao Bolin , Han Luyang , He Yongjun , Cao Xinwei , Li Jianfeng
Published: May 2022

Harmonic noises widely exist in industrial fields and always affect the computational accuracy of neural network models. The existing original adaptive zeroing neural network (OAZNN) model can effectively suppress harmonic noises. Nevertheless, the OAZNN model’s convergence rate only stays at the exponential convergence, that is, its convergence speed is usually greatly affected by the initial state. Consequently, to tackle the above issue, this work combines the dynamic characteristics of harmonic signals with prescribed-time convergence activation function, and proposes a prescribed-time convergent adaptive ZNN (PTCAZNN) for solving time-varying matrix inverse problem (TVMIP) under harmonic noises. Owing to the nonlinear activation function used having the ability to reject noises itself and the adaptive term also being able to compensate the influence of noises, the PTCAZNN model can realize double noise suppression. More importantly, the theoretical analysis of PTCAZNN model with prescribed-time convergence and robustness performance is provided. Finally, by varying a series of conditions such as the frequency of single harmonic noise, the frequency of multi-harmonic noise, and the initial value and the dimension of the matrix, the comparative simulation results further confirm the effectiveness and superiority of the PTCAZNN model.
TECED: A Two-Dimensional Error-Correction Codes Based Energy-Efficiency SRAM Design
Authors: Chen Zhenglin , Zhao Yunping , Lu Jianzhuang , Liang Bin , Chen Xiaowen , Li Chen
Published: May 2022

The reliability of memory is an important issue. The rapid development of transistor technology makes the memory more prone to soft errors. Several recent efforts have proposed various designs to avoid the corruption of stored data by using Error Correction Codes (ECC). However, these designs tend to focus on one indicator, which means they cannot balance the electrical timing, area and power consumption constraints with the increasing of the chip-scale and the operating frequency. In this paper, we propose a design named TECED: A Two-Dimensional Error-Correction Codes Based Energy-Efficiency SRAM Design. We achieve higher energy-efficiency and lower hardware cost by using a two-dimensional error correction codes, and evaluate the design by considering the overall system performance. Comparing with the traditional Hamming code, the evaluation shows that the TECED reduces most of fifty percent of the area overhead and twenty-eight point five percent power consumption of the memory at a specific storage capacity.
Sound Quality Control Based on CEEMD Blind Source Separation and FELMS Algorithm
Authors: Liu Qiang , Zhu Jianxin , Wen Fulin
Published: May 2022

The reduction in sound pressure level is the focus of noise reduction in construction machinery, but the sound quality parameters can better describe the operator’s subjective perception of noise. This paper proposes a sound quality control method for the cab, which is based on complementary ensemble empirical mode decomposition for signal decomposition and reconstruction and an adaptive control algorithm error filter. Firstly, a subjective and objective prediction model was created to identify the target parameters for the sound quality control in the cab. Secondly, the noise was reconstructed based on a complementary ensemble empirical mode decomposition method, thus evaluating the influence of each component on the sound quality and determining the frequency interval. Lastly, the active sound quality control was completed based on the variable step size filter-error least mean square algorithm. The experiments were performed in the cab of a mini-excavator to verify the method’s effectiveness. It was verified that the loudness peak drops by 0.95 sones under stationary idle working conditions. The results demonstrate that the above methods play a guiding role in the actual application of sound quality control for the cab of construction machinery.
Failure Modes, Effects and Criticality Analysis for Wind Turbines Considering Climatic Regions and Comparing Geared and Direct Drive Wind Turbines
Authors: Ozturk Samet , Fthenakis Vasilis , Faulstich Stefan
Published: May 2022

The wind industry is looking for ways to accurately predict reliability and availability of newly installed wind turbines. Failure modes, effects and criticality analysis (FMECA) is a technique utilized to determine the critical subsystems of wind turbines. There are several studies in the literature which have applied FMECA to wind turbines, but no studies so far have used it considering different weather conditions or climatic regions. Furthermore, different wind turbine design types have been analyzed applying FMECA either distinctively or combined, but no study so far has compared the FMECA results for geared and direct-drive wind turbines. We propose to fill these gaps by using Koppen-Geiger climatic regions and two different turbine models of direct-drive and geared-drive concepts. A case study is applied on German wind farms utilizing the Wind Measurement & Evaluation Programme (WMEP) database which contains wind turbine failure data collected between 1989 and 2008. This proposed methodology increases the accuracy of reliability and availability predictions and compares different wind turbine design types and eliminates underestimation of impacts of different weather conditions.
A Two-Stage Biogas Desulfurization Process Using Cellular Concrete Filtration and an Anoxic Biotrickling Filter
Authors: Poser Morgane , Silva Luis Rodolfo Duarte E. , Peu Pascal , Dumont Éric , Couvert Annabelle
Published: May 2022

A two-stage desulfurization process including an abiotic filtration using cellular concrete waste (first stage) and an anoxic biotrickling filter filling with an inoculated expanded schist material (second stage) was investigated to remove H2S in mimic biogas with limited O2 amount (ranged from 0.5 to 0.8%). The two-stage process was able to satisfactorily remove H2S for all experimental conditions (RE > 97%; H2S concentration = 1500 mg m−3; total Empty Bed Residence Time (EBRT) = 200 s; removal capacity (RC) = 26 g m−3 h−1). Moreover, at a total EBRT = 360 s (i.e., 180 s for each stage), the H2S loading rate (LR) was almost treated by the bed of cellular concrete alone, indicating that abiotic filtration could be applied to satisfactorily remove H2S contained in the gas. According to the H2S concentration entering the biotrickling filter, the majority end-product was either elemental sulfur (S0) or sulfate (SO42−). Thus, the ability of the abiotic filter to remove a significant part of H2S would avoid the clogging of the biotrickling filter due to the deposit of S0. Consequently, this two-stage desulfurization process is a promising technology for efficient and economical biogas cleaning adapted to biogas containing limited O2 amounts, such as landfill biogas.
Fluid–Structure Coupling Analysis of the Stationary Structures of a Prototype Pump Turbine during Load Rejection
Authors: He Qilian , Huang Xingxing , Yang Mengqi , Yang Haixia , Bi Huili , Wang Zhengwei
Published: May 2022

During the load rejection transient process of the prototype pump turbine units, the pressure fluctuations of the entire flow passage change drastically due to the rapid closing of guide vanes. The extremely unsteady pressure distribution in the flow domains including the crown chamber and the band chamber may cause a strong vibration on the stationary structures of the unit and result in large dynamic stress on the head cover, stay ring and bottom ring. In this paper, the numerical fluid dynamic analysis of the entire flow passage of a reversible prototype pump turbine during load rejection was performed. The flow characteristics in the runner passage, crown chamber, band chamber, seal labyrinths and balance tubes are analysed. The corresponding unsteady flow-induced dynamic behaviour of the head cover, stay vanes and bottom ring was investigated in detail. The analysed results show that the total deformation of the inner edge of the head cover closed to the main shaft is larger than that of other stationary structures of the unit during the load rejection. The maximum stress of the stay ring is larger than that of the head cover and the bottom ring and the maximum equivalent stress is located at the fillet of the stay vane trailing edge. The fluid–structure coupling calculation method and the analysed results can provide guidance for the design of stationary components of hydraulic machinery such as pump turbines, Francis turbines and centrifugal pumps with different heads.
Comprehensive Optimisation of Biodiesel Production Conditions via Supercritical Methanolysis of Waste Cooking Oil
Authors: Aboelazayem Omar , Gadalla Mamdouh , Saha Basudeb
Published: May 2022

Biodiesel has been established as a promising alternative fuel to petroleum diesel. This study offers a promising energy conversion platform to valorise high acidity waste cooking oil (WCO) into biodiesel in a single-step reaction via supercritical methanol. Carbon dioxide (CO2) has been used as a co-solvent in the reaction with a catalytic effect to enhance the production of biodiesel. This work provides an in-depth assessment of the yield of four fatty acids methyl esters (FAME) from their correspondent triglycerides and fatty acids. The effects of four independent process variables, i.e., methanol to oil (M:O) molar ratio, temperature, pressure, and time, have been investigated using Response Surface Methodology (RSM). Four quadratic models have been developed between process variables and the yield of FAMEs. The statistical validation of the predicted models has been performed using analysis of variance (ANOVA). Numerical optimisation has been employed to predict the optimal conditions for biodiesel production. The predicted optimal conditions are at 25:1 M:O molar ratio, 254.7 °C, 110 bar within 17 min resulting in 99.2%, 99.3%, 99.13%, and 99.05% of methyl-oleate, methyl-palmitate, methyl-linoleate, and methyl-stearate yields, respectively. The predicted optimum conditions have been validated experimentally.
Choice of the Arch Yielding Support for the Preparatory Roadway Located near the Fault
Authors: Skrzypkowski Krzysztof , Zagórski Krzysztof , Zagórska Anna , Apel Derek B. , Wang Jun , Xu Huawei , Guo Lijie
Published: May 2022

The article presents a method of selecting an arch yielding support for preparatory workings driven in a hard coal seam. Particular attention was paid to discontinuous deformation in the form of a fault, which significantly contributes to the change of the excavation protection schemes. On the basis of the geometry of the machines and devices in the designed excavation, the support was selected, which was then checked for the ventilation criterion. In the next stage, analytical calculations were carried out using the determined spacing of the steel support in the fault zone and the area outside of it. Additionally, using the RS3 numerical software based on the finite element method, a rock mass model with a fault was built, through which the preparatory excavation passes. The aim of the research was to determine the total displacements occurring in the fault crossing zone for the excavation without support and with the use of steel arch yielding and with additional reinforcement in the form of straight segments. In conclusion, it was found that the variants of the excavation reinforcement can be modeled and selected in advance, which allows for the fastest possible execution of the driving and the maintenance of the minimum movement dimensions while passing through the fault.
Smart Sustainable Freight Transport for a City Multi-Floor Manufacturing Cluster: A Framework of the Energy Efficiency Monitoring of Electric Vehicle Fleet Charging
Authors: Davydenko Liudmyla , Davydenko Nina , Bosak Andrii , Bosak Alla , Deja Agnieszka , Dzhuguryan Tygran
Published: May 2022

This study focuses on the problem of the efficient energy management of an independent fleet of freight electric vehicles (EVs) providing service to a city multi-floor manufacturing cluster (CMFMC) within a metropolis while considering the requirements of smart sustainable electromobility and the limitations of the power system. The energy efficiency monitoring system is considered an information support tool for the management process. An object-oriented formalization of monitoring information technology is proposed which has a block structure and contains three categories of classes (information acquisition, calculation algorithms, and control procedures). An example of the implementation of the class “Operation with the electrical grid” of information technology is presented. The planning of the freight EVs charging under power limits of the charging station (CS) was carried out using a situational algorithm based on a Fuzzy expert system. The situational algorithm provides for monitoring the charging of a freight EV at a charging station, taking into account the charge weight index (CWI) assigned to it. The optimization of the CS electrical load is carried out from the standpoint of minimizing electricity costs and ensuring the demand for EV charging without going beyond its limits. A computer simulation of the EV charging mode and the CS load was performed. The results of modeling the electrical grid and CS load using the proposed algorithm were compared with the results of modeling using a controlled charging algorithm with electrical grid limitations and an uncontrolled charging algorithm. The proposed approach provides a reduction in power consumption during peak hours of the electrical grid and charging of connected EVs for an on-demand state of charge (SOC).
Growth and Adaptive Capacity of Douglas Fir Genetic Resources from Western Romania under Climate Change
Authors: Mihai Georgeta , Curtu Alexandru-Lucian , Alexandru Alin-Madalin , Nita Ion-Andrei , Ciocîrlan Elena , Birsan Marius-Victor
Published: May 2022

The most recent climate change scenarios show that Southern and Eastern Europe will be affected by a significant increase in temperature and drought frequency by the end of the 21st century. Romania has already recorded very high temperatures and long periods of drought over recent decades, the most affected regions being the south, west and east of the country. Considering that successful forest management requires suitable species and high-quality reproductive material for reforestation, the aim of this study was to evaluate and compare the growth and drought response of Douglas fir (Pseudotsuga menziessi var. menziesii) and Norway spruce (Picea abies) in two stands installed at the end of the 19th century in western Romania. The growth traits, wood characteristics and drought parameters (resistance, recovery, resilience and relative resilience) of Douglas fir and Norway spruce trees have been analyzed and compared. The climate–growth relationship was determined using growth response functions over the period 1938–2017. Additionally, to simulate the potential impact of climate change on Douglas fir in this region, the RCP4.5 scenario was used over two periods: 2041–2070 and 2071–2100. The results reveal that Douglas fir has an exceptional growth capacity, overcoming the Norway spruce since the early ages in both site conditions. The highest growth performances were seen in the low-productivity site. From analyzing the responses to drought events, considerable differences were found between species. The results highlight the high resistance and relative resilience to extreme droughts of Douglas fir compared to Norway spruce. However, autumn–winter temperatures play an important role in the adaptation of Douglas fir to site conditions in Romania. The use of appropriate provenances of Douglas fir in mixed stands with native broadleaved species may be an option for climatically exposed sites, thus increasing the value of these stands.
Assessment of Feed Value of Chicory and Lucerne for Poultry, Determination of Bioaccessibility of Their Polyphenols and Their Effects on Caecal Microbiota
Authors: Iqbal Yasir , Ponnampalam Eric N. , Le Hieu Huu , Artaiz Olivia , Muir Stephanie K. , Jacobs Joe L. , Cottrell Jeremy J. , Dunshea Frank R.
Published: May 2022

Chicory and lucerne possess high feed value for poultry being good sources of protein and fiber. In addition, they are rich in polyphenols that help the body build an integrated antioxidant system to prevent damage from free radicals and positively modulate microbial populations in the gastrointestinal tract. These health-promoting effects of polyphenols depend on their bioaccessibility and absorption in the animal body. The present paper aimed to study the bioaccessibility of polyphenols from chicory and lucerne after subjecting the samples to gastric and intestinal phases of digestion in an in vitro model of chicken gut and assessment of their feed value by measuring the presence of fermentable substrates (in terms of gas production), SCFAs produced and their effects on gut microbiota population during in vitro cecal fermentation. Results revealed that the bioaccessibility of polyphenols varied with different polyphenol compounds. The highest bioaccessibility was recorded for p-hydroxybenzoic acid (90.8%) from chicory following the intestinal phase of digestion. The lowest bioaccessibility was observed for quercetin-3-rhamnoside (12.6%) from chicory after the gastric phase of digestion. From lucerne, the highest bioaccessibility was recorded for kaempferol-3-glucoside (77.5%) after the intestinal phase of digestion. Total gas production was higher for lucerne (39.9 mL/g) than chicory (28.1 mL/g). Similarly, total SCFAs production was higher after 24 h of cecal fermentation with lucerne (42.2 mmol L−1) as compared to chicory (38.1 mmol L−1). Results also revealed that the relative abundance of Clostridium was reduced with chicory (0.225%) and lucerne (0.176%) as compared to the control (0.550%) after 24 h of cecal fermentation. The relative abundance of Streptococcus was reduced by lucerne (4.845%) but was increased with chicory (17.267%) as compared to the control (5.204%) after 24 h of fermentation. These findings indicated that chicory and lucerne differentially affected the microbial populations during in vitro cecal fermentation.
Rapid Production of Fish Sauce from the Internal Organs of White Sturgeon, Acipenser transmontanus Richardson, 1836
Authors: Yanohara Taishi , Taoka Yousuke , Yamamoto Mizuki
Published: May 2022

The internal organs of white sturgeon in Miyazaki Prefecture are discarded during processing. Therefore, we tried to produce fish sauce using a short-term manufacturing method. The minced internal organs were autolyzed by endogenous proteases at 50 °C. During autolysis, the protein contents of the supernatant and precipitate after centrifugation were analyzed by the Kjeldahl method, and the protein size was monitored by SDS-PAGE. This analysis showed that the extraction rate was about 60% after treatment at 50 °C for 24 h. The major bands at 200 kDa, 43 kDa, and 40 kDa detected before the start of the treatment gradually disappeared over time. Fifteen components were detected as the main volatile components. These components increased sharply and then decreased during incubation at 50 °C for 24 h. The fish sauce produced had a good aroma after incubation at 50 °C for 72 h.
Positioning Energy-Neutral Devices: Technological Status and Hybrid RF-Acoustic Experiments
Authors: Cox Bert , Buyle Chesney , Delabie Daan , De Strycker Lieven , Van der Perre Liesbet
Published: May 2022

The digital transformation is exciting the uptake of Internet-of-Things technologies, and raises the questions surrounding our knowledge of the positions of many of these things. A review of indoor localization technologies summarized in this paper shows that with conventional RF-based techniques, a significant challenge exists in terms of achieving good accuracy with a low power consumption at the device side. We present hybrid RF-acoustic approaches as an interesting alternative: the slow propagation speed of sound allows for accurate distance measurements, while RF can easily provide synchronization, data, and power to the devices. We explain how the combination of adequate signaling realizing a late wake-up of the devices with backscattering could position energy-neutral devices. Experiments in a real-life testbed confirmed the potential 10 cm-accuracy based on RF-harvested energy. Nonetheless, these also expose open challenges to be resolved in order to achieve accurate 3D positioning.
Encapsulation of Catechin into β-Glucan Matrix Using Wet Milling and Ultrasonication as a Coupled Approach: Characterization and Bioactivity Retention
Authors: Shah Asima , Ashraf ZanoorUl , Gani Asir , Jhan Faiza , Gani Adil , Sidiq Munazah
Published: May 2022

In this study, the nanoencapsulation of catechin into the β-glucan matrix from oats [O-Glu (C)] and barley [B-Glu (C)] was performed using the coupled approach of ultrasonication and wet milling. The nanoencapsulated catechin was characterised by particle size distribution, surface charge, SEM, and FTIR. The particle size was found to be 200 nm and 500 nm while zeta potential was found −27.13 and −24 mV for O-Glu (C) and B-Glu (C), respectively. The encapsulation efficiency of O-Glu (C) and B-Glu (C) was found to be 86.5% and 88.2%. FTIR and SEM revealed successful entrapment of catechin in β-glucan. The encapsulated capsules showed sustainable release during simulated gastrointestinal conditions. Moreover, both O-Glu (C) and B-Glu (C) showed that biological activity such as lipase inhibition activity and antioxidant assay were retained after in vitro digestion. It was concluded that O-Glu (C) and B-Glu (C) can be used as functional ingredients effectively in food as well as in the pharmaceutical field.
Detection of Resistant and Enterotoxigenic Strains of Staphylococcus warneri Isolated from Food of Animal Origin
Authors: Regecová Ivana , Výrostková Jana , Zigo František , Gregová Gabika , Pipová Monika , Jevinová Pavlina , Becová Jana
Published: May 2022

The topic of this work is the detection of antimicrobial resistance to Staphylococcus warneri strains and the genes encoding staphylococcal enterotoxins. It is considered a potential pathogen that can cause various—mostly inflammatory—diseases in immunosuppressed patients. The experimental part of the paper deals with the isolation of individual isolates from meat samples of Oryctolagus cuniculus, Oncorhynchus mykiss, Scomber scombrus, chicken thigh, beef thigh muscle, pork thigh muscle, and bryndza cheese. In total, 45 isolates were obtained and subjected to phenotypic (plasma coagulase activity, nuclease, pigment, hemolysis, lecithinase, and lipase production) and genotypic analyses to confirm the presence of the S. warneri species. The presence of genes encoding staphylococcal enterotoxins A (three isolates) and D (six isolates) was determined by PCR. Using the Miditech system, the minimum inhibitory concentration for various antibiotics or antibiotics combinations was determined, namely for ampicillin; ampicillin + sulbactam; oxacillin; cefoxitin; piperacillin + tazobactam; erythromycin; clindamycin; linezolid; rifampicin; gentamicin; teicoplanin; vancomycin; trimethoprim; chloramphenicol; tigecycline; moxifloxacin; ciprofloxacin; tetracycline; trimethoprim + sulfonamide; and nitrofurantoin. Resistance to ciprofloxacin and tetracycline was most common (73%). At the same time, out of a total of 45 isolates, 22% of the isolates were confirmed as multi-resistant. Isolates that showed phenotypic resistance to β-lactam antibiotics were subjected to mecA gene detection by PCR.
Monitoring and Forecasting of Key Functions and Technologies for Automated Driving
Authors: Ulrich Christian , Frieske Benjamin , Schmid Stephan A. , Friedrich Horst E.
Published: May 2022

Companies facing transformation in the automotive industry will need to adapt to new trends, technologies and functions, in order to remain competitive. The challenge is to anticipate such trends and to forecast their development over time. The aim of this paper is to develop a methodology that allows us to analyze the temporal development of technologies, taking automated driving as an example. The framework consists of a technological and a functional roadmap. The technology roadmap provides information on the temporal development of 59 technologies based on expert elicitation using a multi-stage Delphi survey and patent analyses. The functional roadmap is derived from a meta-analysis of studies including 209 predictions of the maturity of automated driving functions. The technological and functional roadmaps are merged into a consolidated roadmap, linking the temporal development of technologies and functions. Based on the publication analysis, SAE level 5 is predicted to be market-ready by 2030. Contrasted to the results from the Delphi survey in the technological roadmap, 2030 seems to be too optimistic, however, as some key technologies would not have reached market readiness by this time. As with all forecasts, the proposed framework is not able to accurately predict the future. However, the combination of different forecast approaches enables users to have a more holistic view of future developments than with single forecasting methods.
VALD: The Meeting Point of Data Producers and Data Users
Authors: Ryabchikova Tatiana , Pakhomov Yury , Piskunov Nikolai
Published: May 2022

Vienna Atomic Line Database (VALD) contains data on atomic and molecular energy levels and parameters of spectral lines required for stellar spectra analysis. Hundreds of millions of lines for fine spectral synthesis and for opacity calculations are collected in the present version of VALD (VALD3). Critical evaluation of the data and the diversity of extraction tools support the high popularity of VALD among users. The data model of VALD3 incorporates obligatory links to the bibliography making our database more attractive as a publishing platform for data producers. The VALD data quality and completeness are constantly improving allowing better reproduction of stellar spectra. To illustrate continuous evolution of the data content we present a comparative analysis of the recent experimental and theoretical atomic data for Fe-group elements, which will be included in the next VALD release. This release will also include a possibility for extracting the line data with full isotopic and hyperfine structures.
Transforming Commercial Copper Sulfide into Injectable Hydrogels for Local Photothermal Therapy
Authors: Wang Xiaoran , Yang Zizhen , Meng Zhaowei , Sun Shao-Kai
Published: May 2022

Photothermal therapy (PTT) is a promising local therapy playing an increasingly important role in tumor treatment. To maximize PTT efficacy, various near-infrared photoabsorbers have been developed. Among them, metal sulfides have attracted considerable interest due to the advantages of good stability and high photothermal conversion efficiency. However, the existing synthesis methods of metal-sulfide-based photoabsorbers suffer from the drawbacks of complicated procedures, low raw material utilization, and poor universality. Herein, we proposed a flexible, adjustable strategy capable of transforming commercial metal sulfides into injectable hydrogels for local PTT. We took copper sulfide (CuS) as a typical example, which has intense second-window near-infrared absorption (1064 nm), to systematically investigate its in vitro and in vivo characteristics. CuS hydrogel with good syringeability was synthesized by simply dispersing commercial CuS powders as photoabsorbers in alginate-Ca2+ hydrogel. This synthesis strategy exhibits the unique merits of an ultra-simple synthesizing process, 100% loading efficiency, good biocompatibility, low cost, outstanding photothermal capacity, and good universality. The in vitro experiments indicated that the hydrogel exhibits favorable photothermal heating ability, and it obviously destroyed tumor cells under 1064 nm laser irradiation. After intratumoral administration in vivo, large-sized CuS particles in the hydrogel highly efficiently accumulated in tumor tissues, and robust local PTT was realized under mild laser irradiation (0.3 W/cm2). The developed strategy for the synthesis of CuS hydrogel provides a novel way to utilize commercial metal sulfides for diverse biological applications.
Preparation and Enzyme Degradability of Spherical and Water-Absorbent Gels from Sodium Carboxymethyl Cellulose
Authors: Fujita Sayaka , Tazawa Toshiaki , Kono Hiroyuki
Published: May 2022

To synthesize a biodegradable alternative to spherical polyacrylic acid absorbent resin, spherical hydrogel particles were prepared from carboxymethyl cellulose (CMC) dissolved in an aqueous solution, using ethylene glycol diglycidyl ether (EGDE) as a crosslinking agent. The effect of varying the initial CMC concentration and feed amount of EGDE on the shape, water absorbency, water-holding capacity, and enzyme degradability of the resultant CMC hydrogels was determined. The reaction solution was poured into fluid paraffin, and spherical hydrogel particles were obtained via the shear force from stirring. The shape and diameter of the spherical hydrogel particles in the swollen state depended on the CMC concentration. The spherical hydrogel particles obtained by increasing the amount of EGDE resulted in a decrease in absorbency. Additionally, all the spherical hydrogel particles were degraded by cellulase. Thus, spherical biodegradable hydrogel particles were prepared from CMC, and the particle size and water absorption of the hydrogel could be controlled in the range of 5–18 mm and 30–90 g·g−1 in the swollen state, respectively. As an alternative to conventional superabsorbent polymers, the spherical CMC hydrogels are likely to be useful in industrial and agricultural applications.