bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing
Authors: Nishtala Krishnatej , Panigrahi Trailokyanath , Shetty Rohit , Kumar Dhanananajay , Khamar Pooja , Mohan Rajiv R. , Deshpande Vrushali , Ghosh Arkasubhra
Published: February 2022

The differentiation of keratocytes to fibroblasts and myofibroblasts is an essential requisite during corneal wound closure. The aim of this study is to uncover factors involved in differentiation-dependent alteration in the protein profile of human corneal stromal cells using quantitative proteomics. Human corneal fibroblasts were cultured and differentiated into keratocytes in serum-free media and myofibroblasts through treatment with TGF-β. The protein cell lysates from the donors were tryptic and were digested and labeled using a 3-plex iTRAQ kit. The labeled peptides were subjected to LCMS analysis. Biological functional analysis revealed a set of crucial proteins involved in the differentiation of human corneal stromal cells which were found to be significantly enriched. The selected proteins were further validated by immunohistochemistry. Quantitative proteomics identified key differentially expressed proteins which are involved in cellular signaling pathways. Proteins involved in integrin signaling (Ras-RAP1b, TLN and FN) and SLIT-ROBO pathways (PFN1, CAPR1, PSMA5) as well as extracellular matrix proteins (SERPINH1, SPARC, ITGβ1, CRTAP) showed enhanced expression in corneal fibroblasts and myofibroblasts compared to keratocytes, indicating their possible role in wound healing. Corneal stromal cell differentiation is associated with the activation of diverse molecular pathways critical for the repair of fibroblasts and myofibroblasts. Identified proteins such as profilin 1 and talin could play a tentative role in corneal healing and serve as a potential target to treat corneal fibrosis.
Structure, Oligomerization and Activity Modulation in N-Ribohydrolases
Authors: Degano Massimo
Published: February 2022

Enzymes catalyzing the hydrolysis of the N-glycosidic bond in nucleosides and other ribosides (N-ribohydrolases, NHs) with diverse substrate specificities are found in all kingdoms of life. While the overall NH fold is highly conserved, limited substitutions and insertions can account for differences in substrate selection, catalytic efficiency, and distinct structural features. The NH structural module is also employed in monomeric proteins devoid of enzymatic activity with different physiological roles. The homo-oligomeric quaternary structure of active NHs parallels the different catalytic strategies used by each isozyme, while providing a buttressing effect to maintain the active site geometry and allow the conformational changes required for catalysis. The unique features of the NH catalytic strategy and structure make these proteins attractive targets for diverse therapeutic goals in different diseases.
Acute Prenatal Hypoxia in Rats Affects Physiology and Brain Metabolism in the Offspring, Dependent on Sex and Gestational Age
Authors: Graf Anastasia V. , Maslova Maria V. , Artiukhov Artem V. , Ksenofontov Alexander L. , Aleshin Vasily A. , Bunik Victoria I.
Published: February 2022

Hypoxia is damaging to the fetus, but the developmental impact may vary, with underlying molecular mechanisms unclear. We demonstrate the dependence of physiological and biochemical effects of acute prenatal hypoxia (APH) on sex and gestational age. Compared to control rats, APH on the 10th day of pregnancy (APH-10) increases locomotion in both the male and female offspring, additionally increasing exploratory activity and decreasing anxiety in the males. Compared to APH-10, APH on the 20th day of pregnancy (APH-20) induces less behavioral perturbations. ECG is changed similarly in all offspring only by APH-10. Sexual dimorphism in the APH outcome on behavior is also observed in the brain acetylation system and 2-oxoglutarate dehydrogenase reaction, essential for neurotransmitter metabolism. In view of the perturbed behavior, more biochemical parameters in the brains are assessed after APH-20. Of the six enzymes, APH-20 significantly decreases the malic enzyme activity in both sexes. Among 24 amino acids and dipeptides, APH-20 increases the levels of only three amino acids (Phe, Thr, and Trp) in male offspring, and of seven amino acids (Glu, Gly, Phe, Trp, Ser, Thr, Asn) and carnosine in the female offspring. Thus, a higher reactivity of the brain metabolism to APH stabilizes the behavior. The behavior and brain biochemistry demonstrate sexually dimorphic responses to APH at both gestational stages, whereas the APH effects on ECG depend on gestational age rather than sex.
The Antidepressant Duloxetine Inhibits Platelet Function and Protects against Thrombosis
Authors: Lozano Patricia A. , Alarabi Ahmed B. , Garcia Sarah E. , Boakye Erica T. , Kingbong Hendreta T. , Naddour Elie , Villalobos-García Daniel , Badejo Precious , El-Halawany Medhat S. , Khasawneh Fadi T. , Alshbool Fatima Z.
Published: February 2022

While cardiovascular disease (CVD) is the leading cause of death, major depressive disorder (MDD) is the primary cause of disability, affecting more than 300 million people worldwide. Interestingly, there is evidence that CVD is more prevalent in people with MDD. It is well established that neurotransmitters, namely serotonin and norepinephrine, are involved in the biochemical mechanisms of MDD, and consequently, drugs targeting serotonin-norepinephrine reuptake, such as duloxetine, are commonly prescribed for MDD. In this connection, serotonin and norepinephrine are also known to play critical roles in primary hemostasis. Based on these considerations, we investigated if duloxetine can be repurposed as an antiplatelet medication. Our results-using human and/or mouse platelets show that duloxetine dose-dependently inhibited agonist-induced platelet aggregation, compared to the vehicle control. Furthermore, it also blocked agonist-induced dense and α-granule secretion, integrin αIIbβ3 activation, phosphatidylserine expression, and clot retraction. Moreover duloxetine-treated mice had a significantly prolonged occlusion time. Finally, duloxetine was also found to impair hemostasis. Collectively, our data indicate that the antidepressant duloxetine, which is a serotonin-norepinephrine antagonist, exerts antiplatelet and thromboprotective effects and inhibits hemostasis. Consequently, duloxetine, or a rationally designed derivative, presents potential benefits in the context of CVD, including that associated with MDD.
MicroRNA Signatures in the Upper Urinary Tract Urothelial Carcinoma Scenario: Ready for the Game Changer?
Authors: Cinque Alessandra , Capasso Anna , Vago Riccardo , Floris Matteo , Lee Michael W. , Minnei Roberto , Trevisani Francesco
Published: February 2022

Upper urinary tract urothelial carcinoma (UTUC) represents a minor subgroup of malignancies arising in the urothelium of the renal pelvis or ureter. The estimated annual incidence is around 2 cases per 100,000 people, with a mean age at diagnosis of 73 years. UTUC is more frequently diagnosed in an invasive or metastatic stage. However, even though the incidence of UTUC is not high, UTUC tends to be aggressive and rapidly progressing with a poor prognosis in some patients. A significant challenge in UTUC is ensuring accurate and timely diagnosis, which is complicated by the non-specific nature of symptoms seen at the onset of disease. Moreover, there is a lack of biomarkers capable of identifying the early presence of the malignancy and guide-tailored medical treatment. However, the growing understanding of the molecular biology underlying UTUC has led to the discovery of promising new biomarkers. Among these biomarkers, there is a class of small non-coding RNA biomarkers known as microRNAs (miRNAs) that are particularly promising. In this review, we will analyze the main characteristics of UTUC and focus on microRNAs as possible novel tools that could enter clinical practice in order to optimize the current diagnostic and prognostic algorithm.
Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
Authors: Na Junewoo , Park Byong Seo , Jang Doohyeong , Kim Donggue , Tu Thai Hien , Ryu Youngjae , Ha Chang Man , Koch Marco , Yang Sungchil , Kim Jae Geun , Yang Sunggu
Published: February 2022

The hypothalamic arcuate nucleus (Arc) is a central unit that controls the appetite through the integration of metabolic, hormonal, and neuronal afferent inputs. Agouti-related protein (AgRP), proopiomelanocortin (POMC), and dopaminergic neurons in the Arc differentially regulate feeding behaviors in response to hunger, satiety, and appetite, respectively. At the time of writing, the anatomical and electrophysiological characterization of these three neurons has not yet been intensively explored. Here, we interrogated the overall characterization of AgRP, POMC, and dopaminergic neurons using genetic mouse models, immunohistochemistry, and whole-cell patch recordings. We identified the distinct geographical location and intrinsic properties of each neuron in the Arc with the transgenic lines labelled with cell-specific reporter proteins. Moreover, AgRP, POMC, and dopaminergic neurons had different firing activities to ghrelin and leptin treatments. Ghrelin led to the increased firing rate of dopaminergic and AgRP neurons, and the decreased firing rate of POMC. In sharp contrast, leptin resulted in the decreased firing rate of AgRP neurons and the increased firing rate of POMC neurons, while it did not change the firing rate of dopaminergic neurons in Arc. These findings demonstrate the anatomical and physiological uniqueness of three hypothalamic Arc neurons to appetite control.
Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells
Authors: Smeda Martyna , Galler Kerstin M. , Woelflick Melanie , Rosendahl Andreas , Moehle Christoph , Lenhardt Beate , Buchalla Wolfgang , Widbiller Matthias
Published: February 2022

Both the dental pulp and the apical papilla represent a promising source of mesenchymal stem cells for regenerative endodontic protocols. The aim of this study was to outline molecular biological conformities and differences between dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). Thus, cells were isolated from the pulp and the apical papilla of an extracted molar and analyzed for mesenchymal stem cell markers as well as multi-lineage differentiation. During induced osteogenic differentiation, viability, proliferation, and wound healing assays were performed, and secreted signaling molecules were quantified by enzyme-linked immunosorbent assays (ELISA). Transcriptome-wide gene expression was profiled by microarrays and validated by quantitative reverse transcription PCR (qRT-PCR). Gene regulation was evaluated in the context of culture parameters and functionality. Both cell types expressed mesenchymal stem cell markers and were able to enter various lineages. DPSC and SCAP showed no significant differences in cell viability, proliferation, or migration; however, variations were observed in the profile of secreted molecules. Transcriptome analysis revealed the most significant gene regulation during the differentiation period, and 13 biomarkers were identified whose regulation was essential for both cell types. DPSC and SCAP share many features and their differentiation follows similar patterns. From a molecular biological perspective, both seem to be equally suitable for dental pulp tissue engineering.
Deficiency in Retinal TGFβ Signaling Aggravates Neurodegeneration by Modulating Pro-Apoptotic and MAP Kinase Pathways
Authors: Bielmeier Christina B. , Schmitt Sabrina I. , Kleefeldt Nikolai , Boneva Stefaniya K. , Schlecht Anja , Vallon Mario , Tamm Ernst R. , Hillenkamp Jost , Ergün Süleyman , Neueder Andreas , Braunger Barbara M.
Published: February 2022

Transforming growth factor β (TGFβ) signaling has manifold functions such as regulation of cell growth, differentiation, migration, and apoptosis. Moreover, there is increasing evidence that it also acts in a neuroprotective manner. We recently showed that TGFβ receptor type 2 (Tgfbr2) is upregulated in retinal neurons and Müller cells during retinal degeneration. In this study we investigated if this upregulation of TGFβ signaling would have functional consequences in protecting retinal neurons. To this end, we analyzed the impact of TGFβ signaling on photoreceptor viability using mice with cell type-specific deletion of Tgfbr2 in retinal neurons and Müller cells (Tgfbr2ΔOC) in combination with a genetic model of photoreceptor degeneration (VPP). We examined retinal morphology and the degree of photoreceptor degeneration, as well as alterations of the retinal transcriptome. In summary, retinal morphology was not altered due to TGFβ signaling deficiency. In contrast, VPP-induced photoreceptor degeneration was drastically exacerbated in double mutant mice (Tgfbr2ΔOC; VPP) by induction of pro-apoptotic genes and dysregulation of the MAP kinase pathway. Therefore, TGFβ signaling in retinal neurons and Müller cells exhibits a neuroprotective effect and might pose promising therapeutic options to attenuate photoreceptor degeneration in humans.
Mitochondrial Proteins as Source of Cancer Neoantigens
Authors: Prota Gennaro , Lechuga-Vieco Ana Victoria , De Libero Gennaro
Published: February 2022

In the past decade, anti-tumour immune responses have been successfully exploited to improve the outcome of patients with different cancers. Significant progress has been made in taking advantage of different types of T cell functions for therapeutic purposes. Despite these achievements, only a subset of patients respond favorably to immunotherapy. Therefore, there is a need of novel approaches to improve the effector functions of immune cells and to recognize the major targets of anti-tumour immunity. A major hallmark of cancer is metabolic rewiring associated with switch of mitochondrial functions. These changes are a consequence of high energy demand and increased macromolecular synthesis in cancer cells. Such adaptations in tumour cells might generate novel targets of tumour therapy, including the generation of neoantigens. Here, we review the most recent advances in research on the immune response to mitochondrial proteins in different cellular conditions.
Genome-Wide Identification, Classification, and Expression Analysis of the HD-Zip Transcription Factor Family in Apple (Malus domestica Borkh.)
Authors: Liu Kai , Han Xiaolei , Liang Zhaolin , Yan Jiadi , Cong Peihua , Zhang Caixia
Published: February 2022

Homeodomain-leucine zipper (HD-Zip) family genes are considered to play an important role in plant growth and stress tolerance. However, a genome-wide analysis of HD-Zip genes in apples (Malus domestica Borkh.) has not been performed. We detected 48 MdHDZ genes in the apple genome, and categorized them into three subfamilies on the basis of phylogenetic analysis. The chromosomal locations, gene/protein structures, and physiological and biochemical properties of these genes were analyzed. Synteny analysis revealed that segmental duplications were key in the expansion of the apple HD-Zip family. According to an analysis of cis-regulatory elements and tissue-specific expression patterns, MdHDZ genes may be widely involved in the regulation of apple growth and tolerance to environmental stresses. Furthermore, the transcript levels of apple HD-Zip I and II genes were up-regulated in response to fungal treatments. Expression of apple HD-Zip Ⅲ genes was enhanced during adventitious bud regeneration. This suggested possible roles of these genes in regulating the apple response to fungal infection, as well as adventitious bud regeneration. The current results may help us to better understand the evolution and function of apple HD-ZIP genes, and thus facilitate further research on plant resistance to fungal infection and in vitro regeneration.
The Future of Accounting: How Will Digital Transformation Impact the Sector?
Authors: Gonçalves Maria José Angélico , da Silva Amélia Cristina Ferreira , Ferreira Carina Gonçalves
Published: February 2022

The growing dissemination of digital technologies has had an incomparable impact on many dimensions of today’s civilisation. Digital transformation (DT) redefined the industrial structures and reinvented business models. Hence, in the face of Industry 4.0, financial and accounting services face new threats, challenges, and opportunities. How do the business players in the accounting sector perceive this phenomenon? This paper aims to answer this question by following a qualitative and exploratory approach, applied to three case studies, using semi-structured interviews. The study shows that although digital transformation in Portuguese small and medium-sized accounting service enterprises is just starting, Industry 4.0 technologies, optical character recognition (OCR), artificial intelligence (AI), robotics and enterprise resource planning (ERP) in the cloud were the technologies singled out by respondents. Resistance to change, organisational culture and price seem to be the main barriers to DT in accounting. This paper contributes to a better understanding of the role of accounting and accountants in organisations and society in the context of the digital era. Moreover, it provides practical insights into the potential relationship between technological (specifically digital) development and labour market dynamics for accounting professionals.
Successful Management of Pheochromocytoma Crisis with Cardiogenic Shock by Percutaneous Left Ventricular Assist Device
Authors: Nakamura Makiko , Imamura Teruhiko , Fukui Takuya , Oshima Akira , Ueno Hiroshi , Kinugawa Koichiro
Published: February 2022

Therapeutic strategy utilizing mechanical circulatory supports in patients with pheochromocytoma-related cardiogenic shock remains unestablished. We had a 51-year-old man with acute decompensated heart failure due to pheochromocytoma crisis. He received a percutaneous left ventricular assist device-supported alpha-blocker and intensive fluid infusion therapy, which ameliorated impaired end-organ dysfunction, maintaining hemodynamics and achieving cardiac recovery, followed by the successfully scheduled adrenalectomy. Early suspicion of pheochromocytoma and Impella-supported disease-specific medical management might be a promising bridge to surgery strategy.
The Impact of Tocilizumab on Radiological Changes Assessed by Quantitative Chest CT in Severe COVID-19 Patients
Authors: Anghel Ana-Maria-Jennifer , Niculae Cristian-Mihail , Manea Eliza-Daniela , Lazar Mihai , Popescu Mara , Damalan Anca-Cristina , Bel Adela-Abigaela , Nedelcu Iulia-Maria , Patrascu Raluca-Elena , Hristea Adriana
Published: February 2022

(1) Background: We aimed to analyze the characteristics associated with the in-hospital mortality, describe the early CT changes expressed quantitatively after tocilizumab (TOC), and assess TOC timing according to the oxygen demands. (2) Methods: We retrospectively studied 101 adult patients with severe COVID-19, who received TOC and dexamethasone. The lung involvement was assessed quantitatively using native CT examination before and 7–10 days after TOC administration. (3) Results: The in-hospital mortality was 17.8%. Logistic regression analysis found that interstitial lesions above 50% were associated with death (p = 0.01). The other variables assessed were age (p = 0.1), the presence of comorbidities (p = 0.9), the oxygen flow rate at TOC administration (p = 0.2), FiO2 (p = 0.4), lymphocyte count (p = 0.3), and D-dimers level (p = 0.2). Survivors had a statistically significant improvement at 7–10 days after TOC of interstitial (39.5 vs. 31.6%, p < 0.001), mixt (4.3 vs. 2.3%, p = 0.001) and consolidating (1.7 vs. 1.1%, p = 0.001) lesions. When TOC was administered at a FiO2 ≤ 57.5% (oxygen flow rate ≤ 13 L/min), the associated mortality was significantly lower (4.3% vs. 29.1%, p < 0.05). (4) Conclusions: Quantitative imaging provides valuable information regarding the extent of lung damage which can be used to anticipate the in-hospital mortality. The timing of TOC administration is important and FiO2 could be used as a clinical predictor.
Effects of Exergaming on College Students’ Situational Interest, Self-Efficacy, and Motion Sickness
Authors: Lawrence Madeline , Wan Hung-I , Liu Wenxi , McDonough Daniel , Mishra Shivani , Gao Zan
Published: February 2022

Objective: Given the low levels of physical activity (PA) among U.S. college students, the use of exergaming as a supplement to traditional exercise may promote higher levels of motivation and PA. Therefore, this study’s purpose was to examine the effect of two different exergames on college students’ situational interest (SI), self-efficacy (SE), and equilibrium change (EQC) compared to traditional treadmill walking. Methods: Sixty college students (30 female; Mage = 23.6 ± 4.1 years; MBMI = 23.9 ± 4.0 kg/m2) participated in three separate 20 min exercise sessions: (1) Xbox 360 Kinect Just Dance; (2) Xbox 360 Kinect Reflex Ridge; and (3) traditional treadmill walking at 4.0 mph. Participants’ SI, SE, and EQC were measured after each session using a series of validated surveys. Results: A mixed model analysis of covariance (ANCOVA) with repeated measures evaluated mean differences between exercise sessions for all outcomes. Significant main effects were observed between the three exercise sessions (all p < 0.01). Specifically, Just Dance and Reflex Ridge sessions yielded significantly higher SI scores than treadmill exercise, F (10, 49) = 54.61, p < 0.01, η2 = 0.92. In addition, participants experienced significantly lower EQC in Reflex Ridge than in treadmill exercise, F (2, 58) = 4.26, p = 0.02, η2 = 0.13. No differences were identified for SE. Conclusion: The integration of exergaming into traditional exercise routines may help to promote higher levels of SI but not SE amongst college students. RR exergaming also demonstrated low EQC as compared to traditional exercise. Experimental study designs are warranted to provide additional evidence on the efficacy of exergaming.
Special Issue “Clinical Advances in Chronic Intestinal Diseases Treatment”
Authors: Latorre Eva , Mesonero Jose Emilio
Published: February 2022

During the last decades, the management of patients with chronic intestinal diseases has experienced remarkable progress from both diagnostic and therapeutic point of view [...]
Personalized Prediction of Kidney Function Decline and Network Analysis of the Risk Factors after Kidney Transplantation Using Nationwide Cohort Data
Authors: Hong Moongi Simon , Lee Yu-Ho , Kong Jin-Min , Kwon Oh-Jung , Jung Cheol-Woong , Yang Jaeseok , Kim Myoung-Soo , Han Hyun-Wook , Nam Sang-Min ,   Korean Organ Transplantation Registry Study Group
Published: February 2022

We developed a machine-learning-based model that could predict a decrease in one-year graft function after kidney transplantation, and investigated the risk factors of the decreased function. A total of 4317 cases were included from the Korean Organ Transplant Registry (2014–2019). An XGBoost model was trained to predict the recipient’s one-year estimated glomerular filtration rate (eGFR) below 45 mL/min/1.73 m2 using 112 pre- and peri-transplantation variables. The network of model factors was drawn using inter-factor partial correlations and the statistical significance of each factor. The model with seven features achieved an area under the curve of 0.82, sensitivity of 0.73, and specificity of 0.79. The model prediction was associated with five-year graft and rejection-free survival. Post-transplantation hospitalization >25 days and eGFR ≥ 88.0 were the prominent risk and preventive factors, respectively. Donor age and post-transplantation eGFR < 59.8 were connected to multiple risk factors on the network. Therefore, careful donor–recipient matching in older donors, and avoiding pre-transplantation risk factors, would reduce the risk of graft dysfunction. The model might improve long-term graft outcomes by supporting early detection of graft dysfunction, and proactive risk factor control.
Minimally Invasive Partial vs. Total Adrenalectomy for the Treatment of Unilateral Primary Aldosteronism: A Systematic Review and Meta-Analysis
Authors: Flammia Rocco Simone , Anceschi Umberto , Tufano Antonio , Bologna Eugenio , Proietti Flavia , Bove Alfredo Maria , Misuraca Leonardo , Mastroianni Riccardo , Tirone Giuseppe , Carrara Alessandro , Luciani Lorenzo , Cai Tommaso , Leonardo Costantino , Simone Giuseppe
Published: February 2022

Background: This systematic review and metanalysis was conducted to assess differences between perioperative and functional outcomes in patients undergoing minimally-invasive partial (mi-PA) and total adrenalectomy (mi-TA) for unilateral primary aldosteronism (uPHA). Material and Methods: Multiple scientific databases (PUBMED, Web of Science, and Cochrane Library) were searched up to November 2021 for surgical series comparing mi-PA vs. mi-TA for uPHA according to the PRISMA statement. Primary outcomes of interest were perioperative and functional outcomes. Results: Overall, a total of 802 patients from six eligible studies were identified, with mi-PA and mi-TA performed in 40.4% (n = 324) and 59.6% (n = 478) of cases, respectively. No differences were recorded between the two groups according to number of transfusions, EBL and Clavien–Dindo complications ≥2. Similarly, no differences in clinical success, persistence of postoperative hypokalemia and improvement in HTN were reported between mi-PA and mi-TA. Conclusions: In a uPHA setting, mi-PA and mi-TA provide comparable perioperative and functional outcomes despite the use of mi-PA remains limited to patients with small adenoma size, or hereditary/bilateral disease. Due to limited use of standardized reporting criteria in most of current series, the quest for a superiority of mi-PA over mi-TA in the treatment of uPHA still remains open.
Associations among Ratio of Free Triiodothyronine to Free Thyroxine, Chronic Kidney Disease, and Subclinical Hypothyroidism
Authors: Shimizu Yuji , Kawashiri Shin-Ya , Noguchi Yuko , Nakamichi Seiko , Nagata Yasuhiro , Hayashida Naomi , Maeda Takahiro
Published: February 2022

The ratio of free triiodothyronine (FT3) to free thyroxine (FT4) (FT3/FT4), a maker of peripheral thyroxin deiodination, could indicate activity of thyroid hormone. Since positive association between subclinical hypothyroidism (SCH) and chronic kidney disease (CKD) was reported, clarifying the association among FT3/FT4, SCH, and CKD could be an efficient tool to make a strategy for preventing CKD. A cross-sectional study with 1724 Japanese with normal thyroid hormone was conducted. Significant positive association between SCH and CKD was observed; the adjusted odds ratio (OR) and 95% confidence interval (95% CI) was 2.23 (1.38, 3.59). Even though, FT3/FT4 was found to be inversely associated with CKD whereas positively associated with SCH; the adjusted ORs and 95% CIs for 1 standard deviation (SD) increment of FT3/FT4 were 0.51 (0.35, 0.74) for CKD and 2.40 (1.34, 4.29) for SCH, respectively. FT3/FT4 was also found to be positively associated with SCH without CKD but not those with CKD; 1 SD increment of FT3/FT4 were 3.44 (1.72, 6.91) for SCH without CKD and 1.11 (0.40, 3.06) for SCH with CKD, respectively. Although further investigation is necessary, present study indicates that higher activity of peripheral thyroxin deiodination might have beneficial association on absence of CKD even among SCH which is positively associated with CKD.
Infection of Raccoon Dogs (Nyctereutes procyonoides) from Northern Poland with Gastrointestinal Parasites as a Potential Threat to Human Health
Authors: Pilarczyk Bogumiła M. , Tomza-Marciniak Agnieszka K. , Pilarczyk Renata , Rząd Izabella , Bąkowska Małgorzata J. , Udała Jan M. , Tylkowska Agnieszka , Havryliak Viktoriia
Published: February 2022

The aim of the study was to determinate the prevalence and intensity of infection of raccoon dogs with internal parasites, with a particular emphasis on particular species of helminths known to be dangerous to humans. A total of 96 raccoon dogs were obtained from hunters from September 2018 to October 2021. The digestive tract was taken for examination. The parasitological examination was performed using the dissection methods. The extensity of infection with all internal parasites was 60.3%. The following parasites were found in the tested animals: Echinococcus multilocularis (in 10.42% of animals), Toxocara canis (18.75%), Alaria alata (25.0%), Taenia spp. (19.79%), Uncinaria stenocephala (27.08%), Mesocestoides spp. (54.17%) and Dipylidium caninum (6.25%). The highest mean intensity of infection was demonstrated by A. alata and E. multilocularis then by Mesocestoides spp. This study showed that the raccoon dog from northern Poland is a reservoir host of zoonotic pathogens, such as E. multilocularis, Toxocara canis and Alaria alata. Although the role of the racoon dog as a final host of the life cycle of E. multilocularis is considered of less importance than that of the red fox, this species may increase the risk of echinococcosis in humans, mainly due to its growing population in northern Poland.
Paediatric Behçet’s Disease: A Comprehensive Review with an Emphasis on Monogenic Mimics
Authors: Kul Cinar Ovgu , Romano Micol , Guzel Ferhat , Brogan Paul A. , Demirkaya Erkan
Published: February 2022

Behçet’s disease (BD) is a polygenic condition with a complex immunopathogenetic background and challenging diagnostic and therapeutic concepts. Advances in genomic medicine have provided intriguing insights into disease pathogenesis over the last decade, especially into monogenic mimics of BD. Although a rare condition, paediatric BD should be considered an important differential diagnosis, especially in cases with similar phenotypes. Emerging reports of monogenic mimics have indicated the importance of genetic testing, particularly for those with early-onset, atypical features and familial aggregation. Treatment options ought to be evaluated in a multidisciplinary setting, given the complexity and diverse organ involvement. Owing to the rarity of the condition, there is a paucity of paediatric trials; thus, international collaboration is warranted to provide consensus recommendations for the management of children and young people. Herein, we summarise the current knowledge of the clinical presentation, immunopathogenetic associations and disease mechanisms in patients with paediatric BD and BD-related phenotypes, with particular emphasis on recently identified monogenic mimics.
Mycoplasma pneumoniae Pleural Effusion in Adults
Authors: Kim Chang Ho , Lee Jaehee
Published: February 2022

Parapneumonic effusions often complicate Mycoplasma pneumoniae (MP) pneumonia, contrary to the notion that they are a rare feature of MP infection. Increased research and evidence on MP parapneumonic effusions (MPPE) can help elucidate its clinical significance as one of the variable manifestations of MP infection. This article aims to summarize the existing literature about the clinical characteristics of MPPE in adults and discuss its diagnostic implications from the perspective of pleural fluid analysis. Approximately 20–25% of adult patients with MP pneumonia develop MPPE, and its frequency in children and adults seems to be similar. Although the pathogenesis of MPPE remains to be elucidated, MP-induced cell-mediated immune mechanisms might be partially associated with the development of MPPE. MPPE usually shows mononuclear leukocyte predominance with elevated adenosine deaminase (ADA) activity, similar to tuberculous pleural effusion (TPE). The degree of increase in pleural fluid ADA levels and serum inflammatory biomarkers may help differentiate between MPPE and TPE. During the acute phase, a single positive IgM and positive polymerase chain reaction results allow for a precise and reliable MP infection diagnosis. The mainstay of treatment is the selection of adequate anti-mycoplasma antibiotics with or without corticosteroid, based on the local epidemiologic data on macrolide resistance.
The Influence of Surgical Correction of Idiopathic Scoliosis on the Function of Respiratory Muscles
Authors: Jasiewicz Barbara , Rożek Karina , Kurzeja Piotr , Daszkiewicz Edyta , Ogrodzka-Ciechanowicz Katarzyna
Published: February 2022

Background and objective: It is important to introduce respiratory exercises to the therapy of patients after the surgical treatment of adolescent idiopathic scoliosis. Surgical correction is the best way to prevent hypoxia in scoliosis, but whether pulmonary rehabilitation increases the effectiveness of scoliosis surgery has not yet been confirmed. Therefore, the aim of the study was to evaluate the function of respiratory muscles after surgical correction of idiopathic scoliosis. Methods: The study involved 24 patients, aged 13.6 ± 0.6. Maximum inspiratory pressure (MIP) and maximum expiratory pressure (MEP) were measured using the Mikro RPM. In all patients, before the procedure, 7 days after and 3 months after the procedure, the MIP and MEP were measured. Results: MIP was the lowest 7 days after the procedure; it was 45.28 cmH2O and was statistically significantly lower compared to the measurement before the procedure (p < 0.001) and 3 months after the procedure (p < 0.001). Conclusions: The degree of curvature of the spine before the procedure does not significantly affect initial values of the strength of respiratory muscles. The level of MIP is not dependent on the type of surgery.
Serial Changes of Long COVID Symptoms and Clinical Utility of Serum Antibody Titers for Evaluation of Long COVID
Authors: Sakurada Yasue , Sunada Naruhiko , Honda Hiroyuki , Tokumasu Kazuki , Otsuka Yuki , Nakano Yasuhiro , Hanayama Yoshihisa , Furukawa Masanori , Hagiya Hideharu , Otsuka Fumio
Published: February 2022

Background: Various symptoms persist even after the acute symptoms in about one third of patients with COVID-19. In February 2021, we established an outpatient clinic in a university hospital for patients with long COVID and started medical treatment for sequelae that persisted one month or more after infection. Methods: To determine the key factors that affect the onset and clinical course of sequelae, a retrospective analysis was performed at Okayama University Hospital (Japan) between February and July 2021. We focused on changes in the numbers of symptoms and the background of the patients during a three-month period from the first outpatient visit. We also examined the relationship with SARS-CoV-2 antibody titers. Results: Information was obtained from medical records for 65 patients. The symptoms of sequelae were diverse, with more than 20 types. The most frequent symptoms were general malaise, dysosmia, dysgeusia, sleeplessness, and headache. These symptoms improved in about 60% of the patients after 3 months. Patients who required hospitalization and had a poor condition in the acute phase and patients who received oxygen/dexamethasone therapy had higher antibody titers at the time of consultation. Patients with antibody titers ≥200 U/mL showed significantly fewer improvements in long COVID symptoms in 1 month, but they showed improvements at 3 months after the first visit. Conclusion: Long COVID symptoms were improved at 3 months after the initial visit in more than half of the patients. Serum antibody titers were higher in patients who experienced a severe acute phase, but the serum antibody titers did not seem to be directly related to the long-term persistence of long COVID symptoms.
Efficacy of Intense Pulsed Light Combined Blood Extract Eye Drops for Treatment of Nociceptive Pain in Dry Eye Patients
Authors: Wu Yaying , Mou Yujie , Zhang Yu , Han Yu , Lin Lin , Huo Yanan , Zhu Yirui , Yang Shuo , Huang Xiaodan
Published: February 2022

Purpose: To investigate the efficacy of intense pulsed light (IPL) combined with deproteinized calf blood extract (DCBE) eye drops for dry eye disease (DED) patients with nociceptive ocular pain. Methods: In this prospective, one-center, interventional study, 23 subjects with DED and ocular pain were treated with a combination of IPL and DCBE eye drops for four sessions at a four-week interval. Subjective and objective assessments on nociceptive pain and dry eye were examined and analyzed. Results: The visual analog scale (VAS), ocular surface disease index, ocular pain assessment survey (OPAS), patient health questionnaire-9 items, generalized anxiety disorder (GAD-7), Athens insomnia scale, corneal fluorescein staining score, meibomian gland secretion quality, and expressibility scores were significantly reduced after the treatment. Tear break-up time and Schirmer I test increased significantly. The brand density of corneal nerves and neuropeptide substance P also significantly increased. OPAS, GAD-7, meibomian gland secretion quality, and expressibility scores were essential factors affecting the VAS changes. Conclusions: IPL combined with DCBE drop therapy was effective for DED patients with ocular pain. With such treatment, both DED symptoms and the sensation of ocular pain may be improved.
Antioxidant Effects of Statins by Modulating Nrf2 and Nrf2/HO-1 Signaling in Different Diseases
Authors: Mansouri Atena , Reiner Željko , Ruscica Massimiliano , Tedeschi-Reiner Eugenia , Radbakhsh Shabnam , Bagheri Ekta Mariam , Sahebkar Amirhossein
Published: February 2022

Statins are competitive inhibitors of hydroxymethylglutaryl-CoA (HMG-CoA) reductase and have been used to treat elevated low-density lipoprotein cholesterol (LDL-C) for almost four decades. Antioxidant and anti-inflammatory properties which are independent of the lipid-lowering effects of statins, i.e., their pleiotropic effects, might be beneficial in the prevention or treatment of many diseases. This review discusses the antioxidant effects of statins achieved by modulating the nuclear factor erythroid 2 related factor 2/ heme oxygenase-1 (Nrf2/HO-1) pathway in different organs and diseases. Nrf2 and other proteins involved in the Nrf2/HO-1 signaling pathway have a crucial role in cellular responses to oxidative stress, which is a risk factor for ASCVD. Statins can significantly increase the DNA-binding activity of Nrf2 and induce the expression of its target genes, such as HO-1 and glutathione peroxidase) GPx, (thus protecting the cells against oxidative stress. Antioxidant and anti-inflammatory properties of statins, which are independent of their lipid-lowering effects, could be partly explained by the modulation of the Nrf2/HO-1 pathway.
Mobile Telemedicine Screening for Diabetic Retinopathy Using Nonmydriatic Fundus Photographs in Burgundy: 11 Years of Results
Authors: Charlot Anthony , Baudin Florian , Tessier Mélanie , Lebrize Sarah , Hurand Victoire , Megroian Déborah , Arnould Louis , Ben-Ghezala Inès , Bron Alain Marie , Gabrielle Pierre-Henry , Creuzot-Garcher Catherine
Published: February 2022

We analyzed the results of mobile screening for diabetic retinopathy (DR) using retinal photographs, comparing these results between rural and periurban areas, and before and after the first national COVID-19 pandemic lockdown. The Burgundy Union Régionale des Professionnels de Santé (URPS) has organized an annual DR screening since 2004. The examination, performed by an orthoptist, consisted of taking the patient′s history, intraocular pressure measurement, and taking retinal photographs. After remote transmission, the examinations were interpreted by participating ophthalmologists at the Dijon University Hospital. In September 2016, the screening was open to periurban townships. In 11 years, 10,220 patients were screened: 1420 patients (13.9%) had DR of any type, with an average age of 68.5 (±11.3) years, and 59.2% were men. These patients had a statistically significantly higher glycated hemoglobin level (7.4% vs. 7.0%) and a longer duration of diabetes (13.8 vs. 9.3 years) than patients without DR. When comparing rural and periurban areas and periods before and after the beginning of the COVID-19 pandemic, we did not find any significant difference in the screening results. The results of this study are in line with the average findings of similar studies comparing screening strategies for DR. The early detection of DR can benefit from mobile telemedicine screening, identifying a considerable number of patients at an elevated risk, especially in rural areas where access to ophthalmological care is limited.
Glossiness Index of Objects in Halftone Color Images Based on Structure and Appearance Distortion
Authors: Li Donghui , Tanaka Midori , Horiuchi Takahiko
Published: February 2022

This paper proposes an objective glossiness index for objects in halftone color images. In the proposed index, we consider the characteristics of the human visual system (HVS) and associate the image’s structure distortion and statistical information. According to the difference in the number of strategies adopted by the HVS in judging the difference between images, it is divided into single and multi-strategy modeling. In this study, we advocate multiple strategies to determine glossy or non-glossy quality. We assumed that HVS used different visual mechanisms to evaluate glossy and non-glossy objects. For non-glossy images, the image structure dominated, so the HVS tried to use structural information to judge distortion (a strategy based on structural distortion detection). For glossy images, the glossy appearance dominated; thus, the HVS tried to search for the glossiness difference (an appearance-based strategy). Herein, we present an index for glossiness assessment that attempts to explicitly model the structural dissimilarity and appearance distortion. We used the contrast sensitivity function to account for the mechanism of halftone images when viewed by the human eye. We estimated the structure distortion for the first strategy by using local luminance and contrast masking; meanwhile, local statistics changing in the spatial frequency components for skewness and standard deviation were used to estimate the appearance distortion for the second strategy. Experimental results showed that these two mixed-distortion measurement strategies performed well in consistency with the subjective ratings of glossiness in halftone color images.
A Novel Adaptive Factor-Based H∞ Cubature Kalman Filter for Autonomous Underwater Vehicle
Authors: Zhang Aijun , Wu Yixuan , Zhi Chenbo , Yang Rui
Published: February 2022

In the navigation of an autonomous underwater vehicle (AUV), the positioning accuracy and stability of the navigation system will decrease due to uncertainties such as mobility, inaccuracy of a priori process noise characteristic, and simplification of a dynamic model. In order to solve the above problems, a new, adaptive factor-based H∞ cubature Kalman filter based on a fading factor (AF-H∞CKF) is proposed in this paper. On the one hand, the H∞ game theory provides AF-H∞CKF good robustness in the worst case; on the other hand, the fading factor makes the innovation orthogonal and inflates the predicted error covariance and the Kalman gain, which avoids a decrease in estimation precision in the case of model uncertainty. The simulation and experiment results show that the AF-H∞CKF filter can deal with AUV navigation better than other existing algorithms in the presence of outliers and model uncertainty, which confirms its effectiveness and superiority.
A Process Study of Seiches over Coastal Waters of Shenzhen China after the Passage of Typhoons
Authors: Deng Guotong , Xing Jiuxing , Sheng Jinyu , Chen Shengli
Published: February 2022

Analysis of sea-level observations demonstrates that Typhoons Mawar (2017) and Mangkhut (2018) induced seiches in both Dapeng Bay and Daya Bay near Shenzhen of China, with periods varying from about 3.5 to 4.0 h. Typhoon Mawar (2017) also generated seiches with a period of about 1.2 h. Seiches with such periods in the two bays have not been reported in the past. In this study, we investigate the main processes affecting seiches over these coastal waters using a nested-grid ocean circulation modeling system. The modelled results of typhoon-induced seiches agree well with observations, which indicates that the seiches after the passage of typhoons are dynamically free waves generated by the storm-induced accumulation of water bodies in the two bays. Model sensitivity experiments show that wind directions have an important influence on the type and characteristics of seiches. When the wind stress causes the water body to accumulate in a cross-bay direction, seiches in a closed water body are generated. When the wind stress causes the water body to accumulate in an along-bay direction, seiches in a semi-closed water body are produced. Because of the irregularity of the bathymetry and coastline and variability of wind directions, these two types of seiches can exist simultaneously in the two bays.
Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins
Authors: Leannec-Rialland Valentin , Atanasova Vessela , Chereau Sylvain , Tonk-Rügen Miray , Cabezas-Cruz Alejandro , Richard-Forget Florence
Published: February 2022

Crops are threatened by numerous fungal diseases that can adversely affect the availability and quality of agricultural commodities. In addition, some of these fungal phytopathogens have the capacity to produce mycotoxins that pose a serious health threat to humans and livestock. To facilitate the transition towards sustainable environmentally friendly agriculture, there is an urgent need to develop innovative methods allowing a reduced use of synthetic fungicides while guaranteeing optimal yields and the safety of the harvests. Several defensins have been reported to display antifungal and even—despite being under-studied—antimycotoxin activities and could be promising natural molecules for the development of control strategies. This review analyses pioneering and recent work addressing the bioactivity of defensins towards fungal phytopathogens; the details of approximately 100 active defensins and defensin-like peptides occurring in plants, mammals, fungi and invertebrates are listed. Moreover, the multi-faceted mechanism of action employed by defensins, the opportunity to optimize large-scale production procedures such as their solubility, stability and toxicity to plants and mammals are discussed. Overall, the knowledge gathered within the present review strongly supports the bright future held by defensin-based plant protection solutions while pointing out the obstacles that still need to be overcome to translate defensin-based in vitro research findings into commercial products.