bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Intracellular Peptides of the Central Nervous System of Zebrafish (Danio rerio) in a Parkinson’s Disease Model
Authors: Fiametti Louise O. , Franco Camilla A. , Nunes Leticia O. C. , de Castro Leandro M. , Santos-Filho Norival A.
Published: February 2025

Although peptides have been shown to have biological functions in neurodegenerative diseases, their role in Parkinson’s disease has been understudied. A previous study by our group, which used a 6-hydroxydopamine zebrafish model, suggested that nine intracellular peptides may play a part in this condition. In this context, our aim is to better understand the role of five of these nine peptides. The selection of peptides was made based on their precursor proteins, which are fatty acid binding protein 7, mitochondrial ribosomal protein S36, MARCKS-related protein 1-B, excitatory amino acid transporter 2 and thymosin beta-4. The peptides were chemically synthesized in solid phase and characterized by high-performance liquid chromatography and mass spectrometry. Circular dichroism was performed to determine the secondary structure of each peptide, which showed that all five peptides maintain a random structure in the aqueous solutions that were studied. Two molecules show a helical profile in trifluoroethanol, a known structuring agent. Cell viability by the MTT assay indicates that all five peptides are not cytotoxic in all concentrations tested in both mouse and human cell lines. Behavioral assay using a 6-OHDA zebrafish larvae model suggest that all peptides help in the recovery of motor function with 24 h treatment at two concentrations. Three peptides showed a complete recovery from the 6-OHDA-induced motor impairment. Further studies are needed to better understand the mechanism of action of these peptides and whether they are truly a potential ally against Parkinson’s disease.
Intestinal Microbiota Dysbiosis Role and Bacterial Translocation as a Factor for Septic Risk
Authors: Charitos Ioannis Alexandros , Scacco Salvatore , Cotoia Antonella , Castellaneta Francesca , Castellana Giorgio , Pasqualotto Federico , Venneri Maria , Ferrulli Angela , Aliani Maria , Santacroce Luigi , Carone Mauro
Published: February 2025

The human immune system is closely linked to microbiota such as a complex symbiotic relationship during the coevolution of vertebrates and microorganisms. The transfer of microorganisms from the mother’s microbiota to the newborn begins before birth during gestation and is considered the initial phase of the intestinal microbiota (IM). The gut is an important site where microorganisms can establish colonies. The IM contains polymicrobial communities, which show complex interactions with diet and host immunity. The tendency towards dysbiosis of the intestinal microbiota is influenced by local but also extra-intestinal factors such as inflammatory processes, infections, or a septic state that can aggravate it. Pathogens could trigger an immune response, such as proinflammatory responses. In addition, changes in the host immune system also influence the intestinal community and structure with additional translocation of pathogenic and non-pathogenic bacteria. Finally, local intestinal inflammation has been found to be an important factor in the growth of pathogenic microorganisms, particularly in its role in sepsis. The aim of this article is to be able to detect the current knowledge of the mechanisms that can lead to dysbiosis of the intestinal microbiota and that can cause bacterial translocation with a risk of infection or septic state and vice versa.
The Combined Assessment of CTC and ESR1 Status in Liquid Biopsy Samples Enhances the Clinical Value of Prediction in Metastatic Breast Cancer
Authors: Szostakowska-Rodzos Malgorzata , Grzybowska Ewa A. , Mysliwy Izabella , Zub Renata , Jagiello-Gruszfeld Agnieszka , Rubach Maryna , Konieczna Aleksandra , Fabisiewicz Anna
Published: February 2025

Monitoring of metastatic breast cancer (mBC) is an important issue in the clinical management of patients. Liquid biopsy has become a non-invasive method for detecting and monitoring cancer in body fluids. The presence of circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) in peripheral blood indicates poor prognosis and may contribute to early detection of progression, but assessment of these levels is still not routine clinical management. The main objective of this study was to estimate the frequency and clinical value of the ESR1 and PIK3CA mutations identified in circulating free DNA (cfDNA.) The second goal was to evaluate whether simultaneous evaluation of CTCs and mutation status in cfDNA increases the prognostic value of liquid biopsy. The results of the analysis of the CTC number and ESR1 and PIK3CA mutations in blood collected from 179 patients with metastatic breast cancer show that ESR1 mutations are more frequent in patients with advanced luminal breast cancer regardless of the type of the treatment. ESR1 mutations appear primarily during progression, as no mutations were found in primary tumor samples. The main conclusion of the study is that combined assessment of CTCs and ESR1 status in liquid biopsy may improve the prognostic value of liquid biopsy.
Tear Sampling and Biomarker Discovery: A Robust Workflow for Routine Clinical Applications Using UHPLC-MS/MS and Schirmer Strips
Authors: Comito Rossana , Ciavarella Carmen , Astolfi Gloria , Conti Matteo , Porru Emanuele , Violante Francesco Saverio , Versura Piera
Published: February 2025

Human tear analysis is gaining increasing attention as a non-invasive tool for several applications such as proteomics and biomarker identification in various diseases, including cancer. The choice of the correct sampling method determines the result of the analysis. In this study, we developed and validated a robust method for tear protein quantification using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Tear samples were collected with Schirmer strips, a low-cost and practical tool for tear collection. It is the first time that internal standards have been used to enhance the analytical performance of a method based on Schirmer strips for tear sampling, overcoming the issues widely reported in the literature regarding protein extraction and data reproducibility. Non-human proteins were used for method development, ensuring improved accuracy and analytical precision. The method demonstrated excellent recovery, high sensitivity, and reproducibility. The use of Schirmer strips, combined with this advanced analytical method, highlights their potential as a reliable support for tear protein quantification and biomarker discovery. This study provides a cost-effective and reliable workflow for tear proteome analysis and contributes to the growing field of tear-based diagnostics, making it suitable for routine clinical and research applications in precision medicine.
Bacterial Communities and Resistance and Virulence Genes in Hospital and Community Wastewater: Metagenomic Analysis
Authors: Velazquez-Meza Maria Elena , Galarde-López Miguel , Cornejo-Juárez Patricia , Bobadilla-del-Valle Miriam , Godoy-Lozano Ernestina , Aguilar-Vera Edgar , Carrillo-Quiroz Berta Alicia , Ponce de León-Garduño Alfredo , Velazquez Acosta Consuelo , Alpuche-Aranda Celia Mercedes
Published: February 2025

Metagenomic studies have made it possible to deepen the analysis of the abundance of bacterial populations that carry resistance and virulence determinants in the wastewater environment. In this study, a longitudinal collection of samples of community and hospital wastewater from August 2021 to September 2022 was obtained. Shotgun metagenomic sequencing and bioinformatic analysis were performed to characterize the bacterial abundance, antimicrobial resistance genes (ARGs), plasmids, and virulence factor genes (VFGs) contained in the wastewater. The microbial composition of the community and hospital wastewater showed that the most abundant bacterial phyla detected in all samples were: Proteobacteria, Bacteroides, Firmicutes, Campylobacterota, and Actinobacteria. Seasonal differences in the relative abundances of species, ARGs, plasmids, and VFGs were observed. In this study, a total of 270 ARGs were detected, and it was found that the absolute abundance of ARGs only showed a 39% reduction in the treated wastewater. Furthermore, the ARGs detected in this study were found to encode resistance to antibiotics of the last choice. Our results showed that plasmids carrying resistance genes were more abundant in raw wastewater, and 60% more abundant in hospital wastewater compared to community wastewater. Several of the VFGs detected in this study encode for adhesion, motility, and biofilm formation, which likely allows bacteria to remain and persist in the wastewater environment and survive WWTP treatment systems, thus managing to escape into the environment via treated wastewater.
Living Bacteria: A New Vehicle for Vaccine Delivery in Cancer Immunotherapy
Authors: Yang Min , Zhong Peiluan , Wei Pengcheng
Published: February 2025

Cancer vaccines, aimed at evolving the human immune system to eliminate tumor cells, have long been explored as a method of cancer treatment with significant clinical potential. Traditional delivery systems face significant challenges in directly targeting tumor cells and delivering adequate amounts of antigen due to the hostile tumor microenvironment. Emerging evidence suggests that certain bacteria naturally home in on tumors and modulate antitumor immunity, making bacterial vectors a promising vehicle for precision cancer vaccines. Live bacterial vehicles offer several advantages, including tumor colonization, precise drug delivery, and immune stimulation, making them a compelling option for cancer immunotherapy. In this review, we explore the mechanisms of action behind living bacteria-based vaccines, recent progress in popular bacterial chassis, and strategies for specific payload delivery and biocontainment to ensure safety. These approaches will lay the foundation for developing an affordable, widely applicable cancer vaccine delivery system. This review also discusses the challenges and future opportunities in harnessing bacterial-based vaccines for enhanced therapeutic outcomes in cancer treatment.
Deciphering Arabidopsis Aquaporin Networks: Comparative Analysis of the STRING and BioGRID Interactomes
Authors: Lopez-Zaplana Alvaro
Published: February 2025

Aquaporins are transmembrane proteins that mediate the transport of water, as well as various ions and molecules. In plants, they play a critical role in numerous processes, including stress adaptation, nutrition, cellular communication, and transpiration. Therefore, understanding the function and interactions of these proteins with others—known as interactomes—is of significant agronomic and biological interest. This study aims to analyse the interactome of all aquaporins in Arabidopsis thaliana L. using two distinct databases, STRING and BioGRID. After analysing both interactomes, a wide range of interactions were identified between each aquaporin and a diverse array of proteins, including nutrient transporters for ammonium, potassium, phosphorus, sulphur, copper, and sugars; proteins related to responses to abiotic stresses; proteins mediating vesicle membrane fusion, such as synaptobrevins and syntaxins; ubiquitinases; kinases; and other transmembrane proteins. These extensive connections further underscore the critical importance of aquaporins in numerous biological processes, positioning them as central modulators and integration points for cellular and systemic responses in plants.
Development of TR-19 Cyclotron Parameter Settings for Fully Automated Production of Radiometals with Applications in Nuclear Medicine
Authors: Crăciun Liviu Ștefan , Eșanu Tiberiu Relu , Leonte Radu , Schubert Hermann Anton , Erhan Raul Victor , Niculae Dana
Published: February 2025

At the Radiopharmaceutical Research Center (CCR) of the Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering (IFIN-HH), we operate a TR-19 cyclotron for radio isotope production. To broaden our spectrum of radioisotopes with applications in nuclear medicine, we add a new external beam line towards a state-of-the-art solid target station. Besides practical experience with the implementation of the Comecer ALCEO metal solid targetry system, a new, more efficient way of tuning the beam onto the target and the design of a dedicated neutron local layered shielding are presented.
Concealed Weapon Detection Using Thermal Cameras
Authors: Muñoz Juan D. , Ruiz-Santaquiteria Jesus , Deniz Oscar , Bueno Gloria
Published: February 2025

In an era where security concerns are ever-increasing, the need for advanced technology to detect visible and concealed weapons has become critical. This paper introduces a novel two-stage method for concealed handgun detection, leveraging thermal imaging and deep learning, offering a potential real-world solution for law enforcement and surveillance applications. The approach first detects potential firearms at the frame level and subsequently verifies their association with a detected person, significantly reducing false positives and false negatives. Alarms are triggered only under specific conditions to ensure accurate and reliable detection, with precautionary alerts raised if no person is detected but a firearm is identified. Key contributions include a lightweight algorithm optimized for low-end embedded devices, making it suitable for wearable and mobile applications, and the creation of a tailored thermal dataset for controlled concealment scenarios. The system is implemented on a chest-worn Android smartphone with a miniature thermal camera, enabling hands-free operation. Experimental results validate the method’s effectiveness, achieving an mAP@50-95 of 64.52% on our dataset, improving state-of-the-art methods. By reducing false negatives and improving reliability, this study offers a scalable, practical solution for security applications.
Fungi Assessment in Indoor and Outdoor Environment of Four Selected Hospitals in Peninsular Malaysia
Authors: Ahmad Nurul Izzah , Shahrir Nurul Farehah , Omar Hamdan Anis Syuhada , Kamarudin Nur Amalina , Mohammad Sham Noraishah , Mahmood Jamilah , Leong Yin-Hui , Mohd Tap Ratna
Published: February 2025

Hospital buildings require special attention to protect patients and healthcare workers from hospital-acquired infections and sick building illnesses. This is the first study to assess the prevalence of fungus in indoor air, outdoor air, and their contamination on surfaces at selected locations in four highly contaminated hospitals (A, B, C, and D) in Peninsular Malaysia. A total of 294 indoor air samples, 106 scrapped and 169 swabbed, were collected from July 2019 to August 2020. Bioaerosol concentrations were calculated using the colony-forming unit (CFU)/m3. Molecular identification was performed on the cultures. The internal transcribed spacer (ITS) region in the rRNA gene of the isolates was amplified by PCR. Results showed that fungal burden was in the range between 18 and 2597 CFU/m3. Fungal load in selected locations at Hospital D were in the higher range between 106 and 2597 CFU/m3, with two locations exceeding the national guidelines. Fungal genera were highly identified in air samples (47) compared to swabbed (29) and scrapped (18) samples. The dominant species were C. halotolerans, C. tenuissimum, P. alfredii, P. brevicompactum, P. brocae, P. cataractarum, and A. aculeatus. Fungal loads were higher in the Orthopedic and Oral Surgeon Clinic, the On Call Emergency Room, wards, and pathways.
The Economic Effect of Parks and Community-Managed Open Spaces on Residential House Prices in Baltimore, MD
Authors: Russell Sherry , Kweon Byoung-Suk
Published: February 2025

Urban greenspaces, such as parks and other public vegetated spaces, provide respite from the built environment for residents and visitors. Lesser-known urban greenspaces are community-managed open spaces (CMOSs), such as play lots, community gardens, and memorial gardens. This study investigated the effect of the distance to and size of parks and CMOSs on residential house prices in Baltimore, MD, in 2016–2017 using a hedonic price model. This is the first study of an urban city comparing parks and CMOSs. The study included 21,116 houses sold and revealed that park proximate price premiums ranged from 7.73% to 11.01% for distances of up to a 1/2 mile, and the CMOS proximate price premiums were 8.69% and 8.96% for distances of up to 1/8 and 1/4 miles, respectively. Moreover, both parks and CMOSs revealed a buyer preference of a 1/8 to 1/2 mile distance from these urban greenspaces. Small- to medium-sized parks, less than 9.65 acres, increased house prices by approximately 2.36%, and small CMOSs, less than 0.24 acres, increased house prices by 5.93%. These results confirm that parks and CMOSs provide economic benefits in addition to their social, health, and well-being benefits and suggest that CMOSs are a viable economic development strategy for communities.
Effect of Melt Treatment and Heat Treatment on the Performance of Aluminum Cylinder Heads
Authors: Doty Herbert W. , Samuel Ehab , Samuel Agnes M. , Songmene Victor , Samuel Fawzy H.
Published: February 2025

The present study was performed on real-life I4-aluminum cylinder heads produced industrially by applying the lost foam technique to Al-Si-Mg alloys (356 and 357). This work, in addition, introduces a new Al-Cu alloys coded 220 alloy. The main aim of this study is to analyze the effects of liquid metal treatment on the hardness and tensile properties of such castings. The effects of liquid metal treatment (modification with 200 ppm Sr, grain refining with 150 ppm B and degassing using pure Ar) of the castings produced by the lost foam technique on the tensile strength and hardness properties were evaluated. Hydrogen plays an important role in the formation of porosity. At the same time, the foam mold leaves an impression on the casting surface taking the shape of fine holes. In addition, segregation of hydrogen occurs in front of the solidification front. Thus, the porosity is a combination of hydrogen level and the solidification rate. Gains of 17% and 24% are observed for the hardness and yield strength for alloy 357 compared to alloy 356, caused by the difference in their magnesium (Mg) contents in the sense that, in the T6 heat-treated condition, precipitates in the form of ultra-fine Mg2Si phase particles are formed. The enhancement in the mechanical properties of the used alloy depends mainly of the volume fraction of the precipitated Mg2Si particles. The hardness of alloy 220 increases by 18% and the yield strength by 15% compared to that measured for alloy 356. In this case, the hardening phase Al2Cu is responsible for this increase. Thus, this study demonstrates that liquid metal treatments significantly enhance the hardness and yield strength of Al-Si-Mg and Al-Cu alloys, with the gain attributed to refined microstructures and reduced porosity.
Impact of Treatment Methods on the Surface Properties of the Mg-Containing Zeolite Y
Authors: Biessikirski Andrzej , Kaczmarczyk Grzegorz Piotr , Kolano Malwina , Kaznowska-Opala Karolina , Ruggiero-Mikołajczyk Małgorzata , Gurgul Jacek , Kuterasiński Łukasz
Published: February 2025

In the undertaken research, we investigated the preparation route’s influence mainly on the surface properties of the final form of Mg-containing zeolite Y. The parent zeolite was subjected to modification with aqueous solutions of magnesium nitrate via impregnation, ion-exchange, and ultrasonic techniques, respectively. The results obtained from the Atomic Force Microscopy (AFM), Computer Tomography (CT), and crystallinity evaluations indicated that the method of zeolite modification influenced the physicochemical properties of the studied samples. Wet impregnation caused additional surface roughness, whereas both ion-exchange and sonication led to surface smoothing of the Mg-containing zeolite Y. Nitrogen adsorption analysis indicated no enormous changes in the porosity of Mg-containing zeolite Y, which can be explained by a relatively high resistance of zeolite to interaction with magnesium nitrate aqueous solutions. However, the biggest changes in porosity were observed for Mg-Y prepared via the impregnation technique due to the longest contact between the zeolite and Mg solution.
A Non-Self-Referential Characterization of the Gram–Schmidt Process via Computational Induction
Authors: Chen Ray-Ming
Published: February 2025

The Gram–Schmidt process (GSP) plays an important role in algebra. It provides a theoretical and practical approach for generating an orthonormal basis, QR decomposition, unitary matrices, etc. It also facilitates some applications in the fields of communication, machine learning, feature extraction, etc. The typical GSP is self-referential, while the non-self-referential GSP is based on the Gram determinant, which has exponential complexity. The motivation for this article is to find a way that could convert a set of linearly independent vectors {u→i}j=1n into a set of orthogonal vectors {v→}j=1n via a non-self-referential GSP (NsrGSP). The approach we use is to derive a method that utilizes the recursive property of the standard GSP to retrieve a NsrGSP. The individual orthogonal vector form we obtain is v→k=∑j=1kβ[k→j]u→j, and the collective orthogonal vectors, in a matrix form, are Vk=Uk(BΔk+). This approach could reduce the exponential computational complexity to a polynomial one. It also has a neat representation. To this end, we also apply our approach on a classification problem based on real data. Our method shows the experimental results are much more persuasive than other familiar methods.
Identification of Bioactive Metabolites of Capirona macrophylla by Metabolomic Analysis, Molecular Docking, and In Vitro Antiparasitic Assays
Authors: Evaristo Joseph , Laia Elise de , Tavares Bruna , Mendonça Esdras , Grisostenes Larissa , Rodrigues Caroline , do Nascimento Welington , Garcia Carolina , Guterres Sheila , Nogueira Fábio , Zanchi Fernando , Evaristo Geisa
Published: February 2025

Capirona macrophylla is a Rubiaceae known as “mulateiro”. Ethnobotanical extracts have been used for skin treatment and in the management of leishmaniasis and malaria. Objectives: The metabolites in aqueous extracts from wood bark, leaves, and stems were identified, and their in silico docking and in vitro cellular efficacy against Leishmania amazonensis and Plasmodium falciparum were evaluated. Methods: The extracts were analyzed by UHPLC/HRMSn using untargeted metabolomics approach with MSDial, MSFinder, and GNPS software for metabolite identification and spectra clustering. The most abundant metabolites underwent molecular docking using AutoDock via PyRx, targeting the dihydroorotate dehydrogenase from Leishmania and P. falciparum, and evaluated through molecular dynamics simulations using Gromacs. In vitro biological assays were conducted on 60 HPLC-fractions against these parasites. Results: Metabolomics analysis identified 5100 metabolites in ESI+ and 2839 in ESI− spectra among the “mulateiro” samples. GNPS clustering highlighted large clusters of quercetin and chlorogenic acid groups. The most abundant metabolites were isofraxidin, scopoletin, 5(S)-5-carboxystrictosidine, loliolide, quercetin, quinic acid, caffeoylquinic acid (and isomers), chlorogenic acid, neochlorogenic acid, tryptophan, N-acetyltryptophan, epicatechin, procyanidin, and kaempferol-3-O-robinoside-7-O-rhamnoside. Molecular docking pointed to 3,4-dicaffeoylquinic acid and kaempferol as promising inhibitors. The in vitro assays yielded four active HPLC-fractions against L. amazonensis with IC50 values ranging from 175.2 μg/mL to 194.8 μg/mL, and fraction G29 showed an IC50 of 119.8 μg/mL against P. falciparum. Conclusions: The ethnobotanical use of “mulateiro” wood bark tea as an antimalarial and antileishmanial agent was confirmed through in vitro assays. We speculate that these activities are attributed to linoleic acids and quinic acids.
A Defect Detection Algorithm for Optoelectronic Detectors Utilizing GLV-YOLO
Authors: Zhao Xinfang , Lyu Qinghua , Zeng Hui , Ling Zhuoyi , Zhai Zhongsheng , Lyu Hui , Riffat Saffa , Chen Benyuan , Wang Wanting
Published: February 2025

Photodetectors are indispensable in a multitude of applications, with the detection of surface defects serving as a cornerstone for their production and advancement. To meet the demands of real-time and accurate defect detection, this paper introduces an optimization algorithm based on the GLV-YOLO model tailored for photodetector defect detection in manufacturing settings. The algorithm achieves a reduction in the model complexity and parameter count by incorporating the GhostC3_MSF module. Additionally, it enhances feature extraction capabilities with the integration of the LSKNet_3 attention mechanism. Furthermore, it improves generalization performance through the utilization of the WIoU loss function, which minimizes geometric penalties. The experimental results showed that the proposed algorithm achieved 98.9% accuracy, with 2.1 million parameters and a computational cost of 7.0 GFLOPs. Compared to other methods, our approach outperforms them in both performance and efficiency, fulfilling the real-time and precise defect detection needs of photodetectors.
Performance of Urine Reagent Test Strips in Detecting Schistosoma haematobium Infection in Individual and Pooled Urine Samples
Authors: Degarege Abraham , Erko Berhanu , Brett-Major David M. , Levecke Bruno , Animut Abebe , Negash Yohannes , Broadhurst M. Jana , Michaud Tzeyu , Bilder Christopher R.
Published: February 2025

This study evaluated the performance of urine reagent strips (URSs) in detecting Schistosoma haematobium infection in individual and pooled urine samples. Between June 2022 and April 2023, 2634 urine samples (10 mL each) from school-age children (5–15 years) in 15 villages across Ethiopia’s Afar, Benishangul-Gumuz, and Gambella regions were tested using urine filtration microscopy (UFM) and URSs for blood, a marker of S. haematobium eggs. Pooled samples from 5, 10, 20, and 40 individuals (one positive, others negative) were examined with both methods. UFM results were used to calculate URSs’ sensitivity, specificity, and predictive values for detecting infection. A total of 2634 children were screened for S. haematobium infection. UFM detected S. haematobium eggs in 370 samples, while URSs identified infection in 414 children. URSs showed 64% sensitivity and 92% specificity for individual samples. The positive and negative predictive values for individual samples were 57% and 94%, respectively. Sensitivity for pooled samples ranged from 47% (pools of 40) to 53% (pools of 20). In pools with one positive sample, URSs misclassified 220 (50%), 109 (49.5%), 52 (47.0%), and 28 (50.9%) pools as negative for S. haematobium eggs for pool sizes 5, 10, 20, and 40, respectively. Sensitivity for individual samples was higher in children with heavy infection (92.5%) compared to light infection (55.9%), and sensitivity in pooled samples increased with infection intensity (p < 0.001). In conclusion, URSs may misclassify S. haematobium infection in children when samples are examined individually or in pools, potentially leading to unnecessary treatment or missed cases. However, URSs shows promise as a screening tool for detecting S. haematobium infection in areas with high infection intensity.
Rare Taxa as Key Drivers of Soil Multi-Nutrient Cycling Under Different Crop Types
Authors: Yang Qingmiao , Liu Hanwen , Tang Biao , Yu Chunxiao , Dong Shide , Li Yang , Cui Guangxu , Zhang Yi , Wang Guangmei
Published: February 2025

Soil microorganisms are crucial for nutrient cycling, with abundant and rare taxa playing distinct roles. However, the mechanisms by which soil microbes influence nutrient cycling under different crop types remain unclear. In this study, we investigated the network structure, diversity, and microbial composition of croplands in the Yellow River Delta, focusing on four primary crops: soybean, maize, cotton, and sorghum. The findings revealed that the co-occurring network structure of sorghum planting-soils exhibited greater complexity than other crop types. Bacterial alpha diversity in cotton-planting soil is the highest and susceptible to environmental variations. The diversity of both rare and abundant taxa responds differently to soil nutrients depending on the crop type. While abundant taxa play a crucial role in soil multi-nutrient cycling, rare taxa are key drivers of variations in nutrient cycling expression. The diversity of rare taxa showed a strong correlation with critical nutrients. Structural equation modeling revealed that the alpha diversity of rare bacterial and fungal taxa significantly influenced the soil multi-nutrient cycling index (MNC). Specifically, higher Shannon indices of rare bacterial taxa were associated with lower MNC, while the opposite was true for soil fungi. Soil organic carbon and soil total nitrogen are the key factors influencing alpha diversity in rare bacterial and fungal taxa. Moreover, this study provides new insights into the role of rare soil microbial diversity in the nutrient cycling of agricultural ecosystems.
Unlocking Subsurface Geology: A Case Study with Measure-While-Drilling Data and Machine Learning
Authors: Goldstein Daniel , Aldrich Chris , Shao Quanxi , O’Connor Louisa
Published: February 2025

Bench-scale geological modeling is often uncertain due to limited exploration drilling and geophysical wireline measurements, reducing production efficiency. Measure-While-Drilling (MWD) systems collect drilling data to analyze mining blast hole drill rig performance. Early MWD studies focused on penetration rates to identify rock types. This paper investigates Artificial Intelligence (AI)-based regression models to predict geophysical signatures like density, gamma, magnetic susceptibility, resistivity, and hole diameter using MWD data. The machine learning (ML) models evaluated include Linear Regression (LR), Decision Trees (DTs), Support Vector Machines (SVMs), Random Forests (RFs), Gaussian Processes (GP), and Neural Networks (NNs). An analytical method was validated for accuracy, and a three-tier experimental method assessed the importance of MWD features, revealing no performance loss when excluding features with less than 2% importance. RF, DTs, and GPs outperformed other models, achieving R2 values up to 0.98 with a low RMSE, while LR and SVMs showed lower accuracy. The NN’s performance improved with larger datasets. This study concludes that the DT, RF, and GP models excel in predicting geophysical signatures. While ML-based methods effectively model relationships in the data, their predictive performance remains inherently constrained by the underlying geological and physical mechanisms. Model selection depends on computational resources and application needs, offering valuable insights for real-time orebody analysis using AI. These findings could be invaluable to geologists who wish to utilize AI techniques for real-time orebody analysis and prediction.
Chemical Looping CH4 Reforming Through Isothermal Two-Step Redox Cycling of SrFeO3 Oxygen Carrier in a Tubular Solar Reactor
Authors: Abanades Stéphane , Wang Xinhe , Chuayboon Srirat
Published: February 2025

The chemical looping reforming of methane using an SrFeO3 oxygen carrier to produce synthesis gas from solar energy was experimentally investigated and validated. High-temperature solar heat was used to provide the reaction enthalpy, and therefore the methane feedstock was entirely dedicated to producing syngas. The two-step isothermal process encompassed partial perovskite reduction with methane (partial oxidation of CH4) and exothermic oxidation of SrFeO3-δ with CO2 or H2O splitting under the same operating temperature. The oxygen carrier material was shaped in the form of a reticulated porous foam structure for enhancing heat and mass transfer, and it was cycled in a solar-heated tubular reactor under different operating parameters (temperature: 950–1050 °C, methane mole fraction: 5–30%, and type of oxidant gas: H2O vs. CO2). This study aimed to assess the fuel production capacity of the two-step process and to demonstrate the potential of using strontium ferrite perovskite during solar cycling for the first time. The maximum H2 and CO production rates during CH4-induced reduction were 70 and 25 mL/min at 1000 °C and 15% CH4 mole fraction. The increase in both the cycle temperature and the methane mole fraction promoted the reduction step, thereby enhancing syngas yields up to 569 mL/g during reduction at 1000 °C under 30% CH4 (778 mL/g including both cycle steps), and thus outperforming the performance of the benchmark ceria material. In contrast, the oxidation step was not significantly affected by the experimental conditions and the material’s redox performance was weakly dependent on the nature of the oxidizing gas. The syngas yield remained above 200 mL/g during the oxidation step either with H2O or CO2. Twelve successive redox cycles with stable patterns in the syngas production yields validated material stability. Combining concentrated solar energy and chemical looping reforming was shown to be a promising and sustainable pathway toward carbon-neutral solar fuels.
Endothelial Homeostasis Under the Influence of Alcohol—Relevance to Atherosclerotic Cardiovascular Disease
Authors: Gusti Yusof , Liu Weimin , Athar Fathima , Cahill Paul A. , Redmond Eileen M.
Published: February 2025

Alcohol, in the form of ethyl alcohol or ethanol, is a widely consumed substance with significant implications for human health. Research studies indicate multifaceted effects of alcohol on the cardiovascular system with both protective and harmful effects on atherosclerotic cardiovascular disease (ASCVD), depending on the amount involved and the pattern of consumption. Among the critical components of the cardiovascular system are endothelial cells which line blood vessels. These cells are pivotal in maintaining vessel homeostasis, regulating blood flow, and preventing thrombosis. Their compromised function correlates with arterial disease progression and is predictive of cardiovascular events. Here we review research investigating how alcohol exposure affects the endothelium to gain insight into potential mechanisms mediating alcohol’s influence on ASCVD underlying heart attacks and strokes. Studies highlight opposite effects of low versus high levels of alcohol on many endothelial functions. In general, low-to-moderate levels of alcohol (~5–25 mM) maintain the endothelium in a non-activated state supporting vascular homeostasis, while higher alcohol levels (≥50 mM) lead to endothelial dysfunction and promotes atherosclerosis. These biphasic endothelial effects of alcohol might underlie the varying impacts of different alcohol consumption patterns on ASCVD.
Case Report: Shift from Aggressive Periodontitis to Feline Chronic Gingivostomatitis Is Linked to Increased Microbial Diversity
Authors: Shaw Claire A. , Soltero-Rivera Maria , Profeta Rodrigo , Weimer Bart C.
Published: February 2025

Aggressive Periodontitis (AP) and Feline Chronic Gingivostomatitis (FCGS) are two oral inflammatory diseases in cats with unknown etiology. Both conditions present with severe inflammation of the oral cavity and in FCGS it is found with additional deterioration of the non-keratinized mucosa. The oral microbiome is increasingly implicated in disease progression, but little is known about shifts in the microbial community during the AP and FCGS progression. To that end, we used deep metagenomic sequencing with total RNA on three longitudinal samples of the oral microbiome in a cat first diagnosed with AP that progressed to FCGS. This deep sequencing approach revealed that increased diversity at both the genus and species levels marked the shift from AP to FCGS, including increases in Porphyromonas and Treponema species, and decreased Streptobacillus species. The metatranscriptomes were then probed for expression of antimicrobial resistance genes and virulence factors. Disease-related genes that include cheY, and ompP5 were expressed in early AP and FCGS, while others like galU were only expressed in one or the other disease state. Both genus and species-level shifts were observed along the longitudinal microbiome samples with a noted increase in species diversity in the FCGS-associated microbiome. Corroborating that functional shifts accompany taxonomic changes, the AMR and virulence factor expression similarly changed between the sampling points. Together, these taxonomic and functional shifts indicate that AP and FCGS are potentially linked and may be marked by changes in the oral microbiome, which supports the development of microbial-based clinical diagnostics and therapeutics.
A Combination of Anatolian Propolis and Curcumin Protects Fibroblasts Against Beclomethasone (Nazal Steroid)-Induced Oxidative Stress by Modulating IL-25, MMP-2, VEGF, and FGF-2 Expressions
Authors: Yagci Tarik , Genc Sidika , Dundar Riza , Altiner Halil Ibrahim , Taghizadehghalehjoughi Ali
Published: February 2025

Background: Nasal steroids are commonly prescribed in ear, nose, and throat clinics. It is observed that the use of nasal steroids is increasing due to the prevalence of allergic rhinitis. Because beclomethasone (BCM) toxicity is low, it is highly preferred in allergic rhinitis. The rate of toxicity increases with the increase in the duration and dose of BCM use. However, the protective mechanism of Anatolian propolis (AP) and curcumin (Cur) against BCM toxicity has not been fully explained. Aim: The study evaluates the potential BCM-induced toxicity effect on VEGF, MMP-2, IL-25, and IL-10 parameters after Cur and AP treatment. Materials and Methods: Cell viability, oxidative stress, and gene expression were used for toxicity evaluation. Results: AP 2.5 mg/mL and Cur 16 µg/mL show high viability and antioxidant capacity. BCM increased the levels of IL-25, IL-10, and MMP-2, and a decrease was detected in the expression levels of FGF-2 and VEGF. Conclusions: AP and Cur show effective healing, and AP has been shown to improve inflammation more effectively than Cur. However, the combination of AP and Cur significantly improved the induced toxicity effects.
Global Species Diversity Patterns of Polypodiaceae Under Future Climate Changes
Authors: Huang Sibo , Zhang Gangmin , Dong Wenpan
Published: February 2025

Global change influences species diversity patterns. Compared with seed plants, ferns are more sensitive to temperature and humidity changes and are an ideal group for studying species diversity patterns under future climate changes. Polypodiaceae, which has important ecological and application value, such as medicinal and ornamental value, is one of the most widely distributed fern families, with rich species diversity. Here, we explore the changes in the species diversity patterns of Polypodiaceae and their influencing factors. We collected more than 300,000 data points on the distribution of Polypodiaceae to map actual current species diversity patterns. We used Maxent to establish current and future potential species distribution models using 20 predictors and determined the current species diversity patterns using the actual current species diversity patterns and current potential species distribution model method. Multiple linear regression and random forest models were used to evaluate the effects of climate factors on the species diversity patterns of Polypodiaceae. We evaluated the effects of future climate changes on the species diversity of Polypodiaceae. The species diversity of Polypodiaceae increased gradually from higher to lower latitudes and the centers were concentrated in the low latitudes of tropical rainforests. There were four distribution centers across the world for Polypodiaceae: central America, central Africa, southern Asia, and northern Oceania. The species diversity of Polypodiaceae was greatly affected by precipitation factors rather than temperature factors. Under future climate change scenarios, species diversity is expected to shift and accumulate toward the equator in mid-to-low latitudes. Species diversity is projected to remain concentrated in low-latitude regions but will tend to aggregate towards higher altitude areas as global temperatures rise, with precipitation during the warmest season identified as the most influential factor.
Variations in Acorn Characteristics Between Two Mediterranean Quercus Species and Their Hybrids Through Contrasting Environmental Gradients in Spain
Authors: González-Carrera Santiago , Escudero Alfonso , Fernández-Fuentes Alejandro , Martínez-Ortega Montserrat , Mediavilla Sonia
Published: February 2025

Oaks are characterized by high plasticity and intense interspecific gene flow due to natural hybridization. This generates a wide phenotypic spectrum, which creates taxonomic confusion within the genus. We compared the acorn traits across a temperature gradient in two types of Mediterranean Quercus (Quercus faginea Lam. and Q. pyrenaica Willd.) and their hybrids. Genetic groups were identified using amplified fragment length polymorphism (AFLPs) analysis. Acorns sampled from each of the three genetic groups were used for comparative purposes by means of 15 morphological characteristics. Eight of the traits showed discriminant value among the three groups. The acorn height tended to decrease with decreasing temperatures across the gradient, whereas the acorn width exhibited the opposite response. However, fruit traits allowed discrimination between the three groups, and the differences were consistent in the different zones. Both the number of acorns produced and the individual acorn size were larger for Q. pyrenaica. Hybrids showed intermediate traits between both parent species. Traditionally, the persistence of parental species in the absence of reproductive barriers has been explained by the lower fitness of the hybrids. Our results, however, do not reveal the presence of transgressive characteristics in the hybrids that could justify a lower competitive capacity.
Advances in Conductive Biomaterials for Cardiac Tissue Engineering: Design, Fabrication, and Functional Integration
Authors: Khan Tabrej , Vadivel Gayathri , Ayyasamy Kalaivani , Murugesan Gowtham , Sebaey Tamer A.
Published: February 2025

Heart failure functions as one of the leading global causes of death because it falls under the cardiovascular disease categories. Cardiac tissue engineering advances by developing new tissues to rebuild heart functions in individuals with damaged heart structures as it gives medical treatment possibilities to patients reaching their final stage. Most of the heart tissue consists of cardiomyocytes which make up between 80 to 90 percent of the total organ space. The cardiomyocytes retain their specialized cell structure which includes elongation, but they align to produce contractions as they span into length. After myocardial infarction, doctors need elastic soft platforms to heal the heart tissue because they mimic its natural attributes. Special consideration must be paid to the material selection for appropriate mechanical properties, given that different substances have separate qualities. Stem cell survival becomes higher, and cell differentiation develops more efficiently when a proper scaffold design is implemented, thus enabling tissue repair. Conductive biomaterials demonstrate the best candidate status for cardiac tissue engineering due to their ability to both convey electrical signals and boost biological actions as well as promote cellular communication. Scientists conduct life science research on stem cells because the cells present unique characteristics. Biomaterials with conductive properties within cardiac tissue engineering help the body recover heart tissue while improving the functionality of damaged structures in the myocardium. This article analyzes various conductive biomaterials used in biomedical practices for cardiac tissue healing applications.
A Utilitarian Islamic Jurist: al-Shāṭibī
Authors: Aydın Metin , Cevherli Feyza
Published: February 2025

Utilitarianism is a theory of morality and law that aims for the greatest happiness of the greatest number of people. The two names that come to mind when utilitarianism is mentioned in Western thought are Jeremy Bentham and John Stuart Mill, the founders of the theory. Although this theory emerged in Western philosophy, theories that appeal to the concept of utility as the standard of rightness of moral and legal actions can be found in almost every tradition of thought. One of these traditions is Islamic philosophy. In particular, the theory of maṣlaḥa, which is one of the most important legal theories of Islamic legal thought, is a theory that accepts the concept of utility as a fundamental principle. In this article, we focus on the theory of maṣlaḥa of Abū Isḥāq al-Shāṭibī, one of the most prominent thinkers of the theory of maṣlaḥa. Our aim in this article is to point out the similarities and differences between maṣlaḥa theory and utilitarianism, thereby drawing attention to the functionality of the concept of utility as a standard of right or wrong for ethics and law, regardless of society and period.
The Permanence and Indissolubility of Marriage Against the Background of Deuteronomy 24:1
Authors: Bzdyrak Grzegorz , Kubisiak Przemysław
Published: February 2025

This article is an interdisciplinary study. The authors (a canon lawyer and a biblical theologian) endeavour to examine the text of the Book of Deuteronomy 24:1 through both canonical and exegetical lenses. They look at whether and to what extent it is aligned with the contemporary Catholic teaching on the permanence and indissolubility of marriage. They frame the research problem through a series of questions: Is the analysed text contrary to the Catholic Church’s position on the inadmissibility of divorce? Does it imply consent to divorce? Or does it permit marital separation but solely under specific conditions? First, the authors discuss the Catholic teaching on the permanence and indissolubility of marriage. They highlight a distinction between the two terms. They seek to expose the process of evolution of the institution of marriage from the Creation, i.e., God’s original intention in relation to marriage, through the Old Testament period of “hardness of heart”, i.e., from the original sin to the time of Jesus, to the third stage since Jesus, who restored the original order destroyed by sin and elevated the conjugal bond of two baptized people to the dignity of a sacrament. The authors then examine the concept of marital separation. By its very nature, it does not sever the marital bond. The authors explain the legal grounds for separation, among them adultery and failure to maintain marital fidelity. Next, they conduct an in-depth semantic analysis of the studied text and discuss divorce proceedings in the light of Deuteronomy 24:1. They close the discussion with conclusions. Due to the interdisciplinary nature of the work, the authors relied on the literature from the domains of biblical studies and canon law.