bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection and Recognition of Asynchronous Auditory/Visual Speech: Effects of Age, Hearing Loss, and Talker Accent
Authors: Gordon-Salant Sandra , Schwartz Maya S. , Oppler Kelsey A. , Yeni-Komshian Grace H.
Published: January 2022

This investigation examined age-related differences in auditory-visual (AV) integration as reflected on perceptual judgments of temporally misaligned AV English sentences spoken by native English and native Spanish talkers. In the detection task, it was expected that slowed auditory temporal processing of older participants, relative to younger participants, would be manifest as a shift in the range over which participants would judge asynchronous stimuli as synchronous (referred to as the “AV simultaneity window”). The older participants were also expected to exhibit greater declines in speech recognition for asynchronous AV stimuli than younger participants. Talker accent was hypothesized to influence listener performance, with older listeners exhibiting a greater narrowing of the AV simultaneity window and much poorer recognition of asynchronous AV foreign-accented speech compared to younger listeners. Participant groups included younger and older participants with normal hearing and older participants with hearing loss. Stimuli were video recordings of sentences produced by native English and native Spanish talkers. The video recordings were altered in 50 ms steps by delaying either the audio or video onset. Participants performed a detection task in which they judged whether the sentences were synchronous or asynchronous, and performed a recognition task for multiple synchronous and asynchronous conditions. Both the detection and recognition tasks were conducted at the individualized signal-to-noise ratio (SNR) corresponding to approximately 70% correct speech recognition performance for synchronous AV sentences. Older listeners with and without hearing loss generally showed wider AV simultaneity windows than younger listeners, possibly reflecting slowed auditory temporal processing in auditory lead conditions and reduced sensitivity to asynchrony in auditory lag conditions. However, older and younger listeners were affected similarly by misalignment of auditory and visual signal onsets on the speech recognition task. This suggests that older listeners are negatively impacted by temporal misalignments for speech recognition, even when they do not notice that the stimuli are asynchronous. Overall, the findings show that when listener performance is equated for simultaneous AV speech signals, age effects are apparent in detection judgments but not in recognition of asynchronous speech.
Music Training, and the Ability of Musicians to Harmonize, Are Associated With Enhanced Planning and Problem-Solving
Authors: Winston Jenna L. , Jazwinski Barbara M. , Corey David M. , Colombo Paul J.
Published: January 2022

Music training is associated with enhanced executive function but little is known about the extent to which harmonic aspects of musical training are associated with components of executive function. In the current study, an array of cognitive tests associated with one or more components of executive function, was administered to young adult musicians and non-musicians. To investigate how harmonic aspects of musical training relate to executive function, a test of the ability to compose a four-part harmony was developed and administered to musicians. We tested the working hypothesis that musicians would outperform non-musicians on measures of executive function, and that among musicians, the ability to harmonize would correlate positively with measures of executive function. Results indicate that musicians outperformed non-musicians on the Tower of London task, a measure of planning and problem-solving. Group differences were not detected on tasks more selective for inhibitory control, conflict resolution, or working memory. Among musicians, scores on the harmony assessment were positively correlated with performance of the Tower of London task. Taken together, the current results support a strong relationship between musicianship and planning and problem solving abilities, and indicate that the ability to harmonize is associated with components of executive function contributing to planning and problem solving.
Family and Psychosocial Functioning in Bipolar Disorder: The Mediating Effects of Social Support, Resilience and Suicidal Ideation
Authors: Dou Wenbo , Yu Xueying , Fang Hengying , Lu Dali , Cai Lirong , Zhu Caihong , Zong Kunlun , Zheng Yingjun , Lin Xiaoling
Published: January 2022

Patients with bipolar disorder (BD) may experience family dysfunction, which might result in worse psychosocial functioning through environmental and psychological factors. Research investigating the mediating role of social support, resilience and suicidal ideation on family and psychosocial functioning in BD is rare. The study aims to explore the predicting and mediating effects of social support, resilience and suicidal ideation on family and psychosocial functioning in BD patients. Two hundred forty-six patients with BD and sixty-nine healthy controls were recruited. The Family Assessment Device (FAD), Functioning Assessment Short Test (FAST), Social Support Rating Scale (SSRS), Connor-Davidson Resilience Scale (CD-RISC) and Beck Scale for Suicide Ideation (BSI) were used to assess family functioning, psychosocial functioning, social support, resilience and suicidal ideation, respectively. Bipolar patients exhibited worse family and psychosocial functioning than healthy controls. Family functioning, social support, resilience and suicidal ideation significantly predict psychosocial functioning in the bipolar group. Social support, resilience and suicidal ideation indirectly mediate the effect of family functioning on psychosocial functioning in bipolar patients. Cross-sectional design and mixed sample including acute and remitted stages. Treatments for patients with bipolar disorder should be combined with family strategies that are formulated to improve psychosocial functioning. An emphasis should be placed on enhancing social support and resilience while paying attention to suicidal ideation.
The Psychological Impact of Participation in Victim-Offender Mediation on Offenders: Evidence for Increased Compunction and Victim Empathy
Authors: Jonas Jiska , Zebel Sven , Claessen Jacques , Nelen Hans
Published: January 2022

Participation in victim-offender mediation (VOM) can reduce the risk of reoffending. However, relatively little is known about how VOM affects the intermediate psychological changes underlying this effect. It was hypothesized that VOM increases feelings of responsibility, guilt, and shame among offenders as well as empathy toward the victim. It was also expected that VOM leads to feelings of moral failure among offenders, increasing their intention to desist, and improving their relation with the victim, relatives, and community. Lastly, it was hypothesized that offenders may experience reduced rejection, concerns about condemnation, threat to their social moral identity, and victim blame following VOM. To this end, we compared psychological changes in offenders who participated in a VOM program in the Netherlands with those of offenders who were willing to but did not participate (total N = 86). A quasi-experimental, pre- and postmeasure research design was used to compare these groups. Our findings tentatively suggest that offenders who participate in VOM have more responsibility-taking and victim empathy, feel more guilt and shame, and experience higher moral failure than offenders who do not participate in VOM do. Offenders also reported feeling significantly less awkward about meeting the victim again after VOM. Future research should address how and to what degree these psychological changes translate into a lower risk of reoffending.
Exploring the Pharmaceutical Care of Pharmacists in China During COVID-19—A National Multicenter Qualitative Study
Authors: Wang Mingxia , Yin Zhao , Zhang Wan , Jia Xuedong , Du Shuzhang , Li Jun , Zhang Xiaojian
Published: January 2022

Background

Pharmacists are one of the coronavirus disease-2019 (COVID-19) treatment team members in China, yet only a few single-center studies have described the work experience of pharmacists during the pandemic.

Purpose

This study aimed to explore in-depth experiences of hospital pharmacists providing pharmacy services during the COVID-19 pandemic in China on a national scale.

Methods

This is a nationwide multicenter qualitative study that used the purposive sampling method. Semi-structured interviews were conducted with 11 pharmacists from large-scale tertiary hospitals in representative provinces of mainland China. The Colaizzi seven-step method was applied to analyze the interview data.

Results

Eleven semi-structured interviews were conducted. Each interview lasted 25–70 min. By analyzing the work experiences of pharmacists in COVID-19 designated treatment hospitals, five descriptive themes were categorized: (1) drug supply service; (2) routine clinical pharmacy services; (3) expanded pharmacy services during the epidemic; (4) drug management loopholes; (5) areas of improvements of pharmacy services during a pandemic.

Conclusion

During the COVID-19 epidemic, Chinese hospital pharmacists played various vital roles. However, there were loopholes in managing narcotic drugs, psychotropic drugs, and donated drugs. The study uncovered areas of improvement in pharmacy services during the pandemic. The emergency response capacity of hospital pharmacists should be continuously improved in the future.
Robotic Assembly of Timber Structures in a Human-Robot Collaboration Setup
Authors: Kramberger Aljaz , Kunic Anja , Iturrate Iñigo , Sloth Christoffer , Naboni Roberto , Schlette Christian
Published: January 2022

The construction sector is investigating wood as a highly sustainable material for fabrication of architectural elements. Several researchers in the field of construction are currently designing novel timber structures as well as novel solutions for fabricating such structures, i.e. robot technologies which allow for automation of a domain dominated by skilled craftsman. In this paper, we present a framework for closing the loop between the design and robotic assembly of timber structures. On one hand, we illustrate an extended automation process that incorporates learning by demonstration to learn and execute a complex assembly of an interlocking wooden joint. On the other hand, we describe a design case study that builds upon the specificity of this process, to achieve new designs of construction elements, which were previously only possible to be assembled by skilled craftsmen. The paper provides an overview of a process with different levels of focus, from the integration of a digital twin to timber joint design and the robotic assembly execution, to the development of a flexible robotic setup and novel assembly procedures for dealing with the complexity of the designed timber joints. We discuss synergistic results on both robotic and construction design innovation, with an outlook on future developments.
Salvage Surgery in Recurrent Oral Squamous Cell Carcinoma
Authors: Shetty K. S. Rathan , Kurle Vinayak , Greeshma P. , Ganga Veena B. , Murthy Samskruthi P. , Thammaiah Siddappa K. , Prasad P. Krishna , Chavan Purushottham , Halkud Rajshekar , Krishnappa R.
Published: January 2022

More than half of patients with oral cancer recur even after multimodality treatment and recurrent oral cancers carry a poorer prognosis when compared to other sites of head and neck. The best survival outcome in a recurrent setting is achieved by salvage surgery; however, objective criteria to select an ideal candidate for salvage surgery is difficult to frame, as the outcome depends on various treatment-, tumor-, and patient-related factors. The following is summarizes various tumor- and treatment-related factors that guide our decision-making to optimize oncologic and functional outcomes in surgical salvage for recurrent oral cancers. Short disease-free interval, advanced tumor stage (recurrent and primary), extracapsular spread and positive tumor margins in a recurrent tumor, regional recurrence, and multimodality treatment of primary tumor all portend worse outcomes after surgical salvage. Quality of life after surgical intervention has shown improvement over 1 year with a drastic drop in pain scores. Various trials are underway evaluating the combination of immunotherapy and surgical salvage in recurrent head and neck tumors, including oral cavity, which may widen our indications for salvage surgery with improved survival and preserved organ function.
Are Noisy Hospitals Making Us Sick?
Authors: Chatzilampri Georgios , Stevens Frank
Published: January 2022

If you have ever been woken up by noise in the night, you will know just how annoying it can be. But noise does not only disturb us; it can actually be bad for our health. People can be affected by noise in many environments and, unfortunately, hospitals that are occupied by the most vulnerable groups of people are no exception. Studies show that people trying to recover need peace and quiet. But despite all the “quiet please” signs, noise in hospitals is one of the main reason patients cannot sleep, besides physical pain. In this article, we explore how noise affects people and how it impacts those trying to get well in hospitals.
Microbial Survival in an Extreme Martian Analog Ecosystem: Poás Volcano, Costa Rica
Authors: Wang Justin L. , Dragone Nicholas B. , Avard Geoffroy , Hynek Brian M.
Published: January 2022

Past acid-sulfate hydrothermal systems on Mars have promise in their ability to have hosted life for billions of years. One method for analyzing these systems is to study analog environments on Earth. To assess the astrobiological potential of Martian acid-sulfate hydrothermal systems, the crater lake of the active Poás Volcano, Laguna Caliente, was sampled in 2013 and 2017. Laguna Caliente presents an extremely dynamic terrestrial environment with near-ambient to boiling temperatures, pH fluctuations from −0.87 to 1.5, a wide range of chemistries and redox potential, and frequent phreatic-to-phreatomagmatic eruptions. Samples of lake fluid, sulfur clumps, and lake bottom sediment underwent 16S rRNA gene sequencing and metagenomic “shotgun” sequencing, which revealed this lake hosts an extremely low biodiversity of microorganisms dominated by Acidiphilium spp. Shotgun metagenomics of the samples suggests this community has numerous genetic adaptations that confer survival, including functional pathways to reduce the effects of toxic metals and numerous metabolic pathways utilizing a variety of simple and complex sugar molecules. The identification of these various metabolic pathways suggests adaptations related to carbon limited environments, fulfillment of high energy requirements, and survival in a hostile volcanic setting. The perseverance of life in Laguna Caliente indicates life on Mars could have thrived in analogous environments, stressing the need for the search for life in relict Martian acid-sulfate hydrothermal systems.
Phenolics-Linked Antioxidant and Anti-hyperglycemic Properties of Edible Roselle (Hibiscus sabdariffa Linn.) Calyces Targeting Type 2 Diabetes Nutraceutical Benefits in vitro
Authors: Banwo Kolawole , Sanni Abiodun , Sarkar Dipayan , Ale Oluwatosin , Shetty Kalidas
Published: January 2022

Phenolics- enriched plant food sources are excellent dietary and therapeutic targets to combat the increasing prevalence of diet and lifestyle-influenced non-communicable chronic diseases (NCDs), such as type 2 diabetes (T2D). Among plant sources, edible flowers rich in health protective phenolic compounds provide novel opportunities as ingredient and nutraceutical sources. Roselle (Hibiscus sabdariffa Linn.) is a popular edible flower and consumed as part of traditional cuisines and processed foods in several countries of Asia and Africa. Red calyces of Roselle are rich in phenolic compounds, which potentially have high antioxidant and anti-hyperglycemic properties. Therefore, there is merit in screening of dried Roselle calyces as sources for functional food ingredients or nutraceuticals to counter chronic oxidative stress and chronic hyperglycemia using in vitro assays. This has led to this study to investigate and compare phenolic compounds associated antioxidant and anti-hyperglycemic functions of different organic solvent-extracted fractions of dried Roselle calyces using rapid in vitro assays-based screening strategy. Total soluble phenolic content, profile of phenolic compounds, free radical scavenging assay-based total antioxidant activity, and anti-hyperglycemic function linked α-amylase and α-glucosidase inhibitory activities of four different organic solvents (chloroform, hexane, ethyl acetate, and initial crude extraction in 100% methanol) extracted fractions of calyces of Roselle were determined using in vitro assays. Studies indicated high phenolic-linked antioxidant and anti-hyperglycemic relevant properties in red Roselle calyces, specifically in ethyl acetate and methanol solvent-based extracted fractions. Major phenolic compounds in extracted fractions of Roselle calyces were chlorogenic acid, caffeic acid, gallic acid, catechin, rutin, benzoic acid, and cinnamic acid. Additionally, moderate α-amylase (30–92%) and very high α-glucosidase (81–98%) inhibitory activities were confirmed in undiluted samples of organic solvent-extracted fractions of Roselle calyces in the in vitro assays. Taken together these in vitro screening results indicated that calyces of Roselle are excellent sources of health protective phenolic compounds with high antioxidant and anti-hyperglycemic functions and organic solvent (ethyl acetate and methanol) extracted fractions of this edible flower can be strategically utilized to design functional food ingredients and nutraceuticals.
Hermetic Bags for the Storage of Maize: Perspectives on Economics, Food Security and Greenhouse Gas Emissions in Different Sub-Saharan African Countries
Authors: Dijkink Bert , Broeze Jan , Vollebregt Martijntje
Published: January 2022

During storage, cereals and legumes are vulnerable to insects, rodents, and fungi, which can cause loss of weight, damage or discoloration of products, and/or toxin formation. Hermetic bags can prevent excessive insect infestation, and toxin formation. This paper presents an analysis of the effects of hermetic bags for the storage of maize on food loss reduction by insects and on net greenhouse gas (GHG) emissions, based on practical data from field trials. Their economic feasibility, by optimizing the total income in markets with different price seasonalities in different Sub-Saharan African countries, is analyzed. The data of five field trials were combined and put in classes of 50 days of increasing storage time to get a realistic loss of produce during storage using regular bags (with or without the use of pesticides) and hermetic bags. The maize for storage trials were used as is, bought locally or used direct from the field. Scenario studies with standard storage, standard storage combined with pesticides and hermetic bag storage show significant losses by insects after 100 storage days for standard and standard with pesticides storage, whereas with hermetic bags, product losses are kept to a minimum of 2%. The economic analysis shows less clear-cut outcomes: the interventions' effectiveness depends largely on the rate of seasonal price fluctuation of the commodity. For farmers' personal consumption, when the quality is less critical, the use of hermetic bags is only more economical compared with other methods of storage for produce kept over 100 days. Since the quality of maize is well-preserved by the hermetic bag, the return on the investment is shorter when the maize is sold at the market. However, for countries with a low seasonal price gap, the investment cannot be recouped. As the use of hermetic bags is a good intervention for preventing food loss, it is best promoted not only for providing direct profits to farmers but also for health benefits, as bag use implies a lower need for pesticides and a possible reduction in aflatoxin intake.
Survival Analysis in Gastrointestinal Neuroendocrine Carcinoma With Bone Metastasis at Diagnosis
Authors: Chen Genlian , Xu Qiang , Qian Shengjun , Wang Zhan , Wang Shicheng
Published: January 2022

Purpose

Gastrointestinal neuroendocrine carcinoma (NEC) with bone metastasis is rarely reported. The purpose of this study is to explore the prognosis and risk factors of such patients.

Patients and Methods

We retrospectively reviewed patients diagnosed as gastrointestinal NECs with bone metastasis at diagnosis from 2010 to 2016 by using the Surveillance, Epidemiology and End Results (SEER) database. Predictors of overall survival (OS) and cancer-specific survival (CSS) were analyzed by univariable and multivariable Cox analyses. Kaplan–Meier plots were constructed to show the correlation between independent predictors and survival.

Results

A total of 330 gastrointestinal NEC patients with bone metastasis at diagnosis were included for analysis. Over half of patients were male and older than 60 years old. The most common primary site of gastrointestinal NEC with bone metastasis was the pancreas. The prognosis of gastrointestinal NEC with bone metastasis (3-year OS and CSS rates: 16.7 and 17.0%) was very poor. On Cox multivariable analysis, age over 60 years old, no surgery, and lung metastasis were independent predictors of decreased OS and CSS.

Conclusions

We identified three independent factors associated with prognosis among gastrointestinal NEC patients with bone metastasis, namely age, surgery, and lung metastasis. For younger gastrointestinal NEC patients with bone metastasis, surgical resection of primary tumors as well as actively treating lung metastasis might be useful for prolonging survival.
Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
Authors: Kim Sang-Won , Choi Yu-Ri , Park Jong-Yeol , Wei Bai , Shang Ke , Zhang Jun-Feng , Jang Hyung-Kwan , Cha Se-Yeoun , Kang Min
Published: January 2022

Avian reoviruses (ARVs) cause severe arthritis, tenosynovitis, pericarditis, and depressed growth in chickens, and these conditions have become increasingly frequent in recent years. Studies on the role of wild birds in the epidemiology of ARVs are insufficient. This study provides information about currently circulating ARVs in wild birds by gene detection using diagnostic RT-PCR, virus isolation, and genomic characterization. In this study, we isolated and identified 10 ARV isolates from 7,390 wild birds' fecal samples, including migratory bird species (bean goose, Eurasian teal, Indian spot-billed duck, and mallard duck) from 2015 to 2019 in South Korea. On comparing the amino acid sequences of the σC-encoding gene, most isolates, except A18-13, shared higher sequence similarity with the commercial vaccine isolate S1133 and Chinese isolates. However, the A18-13 isolate is similar to live attenuated vaccine av-S1133 and vaccine break isolates (SD09-1, LN09-1, and GX110116). For the p10- and p17-encoding genes, all isolates have identical fusion associated small transmembrane (FAST) protein and nuclear localization signal (SNL) motif to chicken-origin ARVs. Phylogenetic analysis of the amino acid sequences of the σC-encoding gene revealed that all isolates were belonged to genotypic cluster I. For the p10- and p17-encoding genes, the nucleotide sequences of all isolates indicated close relationship with commercial vaccine isolate S1133 and Chinese isolates. For the σNS-encoding gene, the nucleotide sequences of all isolates indicated close relationship with the Californian chicken-origin isolate K1600657 and belonged to chicken-origin ARV cluster. Our data indicates that wild birds ARVs were derived from the chicken farms. This finding suggests that wild birds serve as natural carriers of such viruses for domestic poultry.
Leaching of Glyphosate and AMPA from Field Lysimeters
Authors: Milan Marco , Vidotto Francesco , Fogliatto Silvia
Published: January 2022

Leaching of glyphosate and AMPA as affected by the time elapsed between the spraying and first leaching event was studied on large-scale lysimeters in a two-year study. The leaching events were induced by irrigation interventions able to deliver 336 L, equivalent to a rainfall of 40 mm. Four groups of three lysimeters were randomly selected between the 12 lysimeters available. They were irrigated on either one day after herbicide treatment (1 DAT), 7 DAT, 14 DAT or 28 DAT. The same group of lysimeters were irrigated a second time 14 days after the first irrigation, corresponding to a period of time of 15 DAT (1 + 14), 21 DAT (7 + 14), 28 DAT (14 + 14) and 42 DAT (28 + 14). In both years, lysimeters were sprayed with glyphosate (360 L ha−1) at a rate of 12 L ha−1, the maximum field rate allowed on the label. Our results pointed out that the leaching of glyphosate and AMPA is effectively event-driven and highlighted the importance of the first rainfall event in moving glyphosate through the soil, increasing the potential risk of water contamination. Overall, both chemicals showed a risk of water contamination. Glyphosate may persist more than usually considered, and its residues were found in leached waters from lysimeters treated 30 days before the leaching event. Other factors may affect the movement of these two compounds through the soil profile after spraying: temperature pattern and soil moisture. Finally, the results of this study refer to a very high application rate of glyphosate. Hence, at lower field rates, observed concentrations can likely be minor.
Development of Quantitative Real-Time PCR Assays to Quantify Erysiphe pisi and Erysiphe trifolii and Its Implementation for Monitoring Their Relative Prevalence in Pea Crops in Spain and Tunisia
Authors: Fondevilla Sara , González-Bernal Mª José , Omri Ben Youssef Noura , Rubiales Diego
Published: January 2022

E. pisi was thought to be the only causal agent of powdery mildew in peas, with three genes, er1, er2 and Er3, conferring resistance to this pathogen. Recently, E. trifolii has also been found to cause this disease in peas in different countries, but its relevance in pea powdery mildew disease worldwide is unknown. The objective of this study was to develop a method to identify and quantify E. pisi and E. trifolii and use it to analyze the relative prevalence of E. pisi and E. trifolii in pea fields in Spain and Tunisia. We also wanted to discern the effect of the er1, er2 and Er3 resistance genes on the relative amount of E. pisi/E. trifolii. Using the polymorphic sites present between E. pisi and E. trifolii ITS sequences, we developed a qPCR method capable of identifying and quantifying these pathogens. Our results revealed, for the first time, the occurrence of E. trifolii in Tunisia and that the presence of er1, er2 and Er3 genes have a clear effect on the ratio E. pisi/E. trifolii in both countries.
Optimalisation of the Activation Medium and Effect of Inhibiting Activities of Acid Phosphatase, Lactate Dehydrogenase and β-N-Acetylglucosaminidase on the Fertilisation Success of Eurasian Perch (Perca fluviatilis L.)
Authors: Sarosiek Beata , Dryl Katarzyna , Kowalski Radosław K. , Palińska-Żarska Katarzyna , Żarski Daniel
Published: January 2022

Although methods for the artificial reproduction of perch have been developed, a lack of information remains regarding the enzymes present in its semen, as well as their role in the fertilisation process. In this study, we first select the optimal activating solution for perch fertilisation and then determine the inhibition effect of enzymes that have already been reported as present in the sperm of teleosts—acid phosphatase (AcP), lactic dehydrogenase (LDH) and β-N-acetylglucosaminidase (β-NAGase)—on the percentage of motile spermatozoa and fertilised eggs. Of the 8 studied activation media, a solution composed of 80 mM NaCl, 20 mM KCl, 10 mM Tris, with pH 8.0 and 206 mOsm/kg proved to be optimal for perch gametes. The addition of ammonium molybdate (AcP inhibitor) caused no significant reduction in the percentage of fertilised eggs. On the other hand, the addition of 0.25 mM gossypol (LDH inhibitor) and 0.125 M acetamide (β-N-acetylglucosaminidase inhibitor) significantly decreased the fertilisation percentage to 41.1% and 52.4%, respectively, in contrast to the control (89.9 %). Both LDH and β-NAGase thus seem to play a very important role in the perch fertilisation process.
Assessing Two Different Aerial Toxin Treatments for the Management of Invasive Rats
Authors: O’Malley Tess D. R. , Stanley Margaret C. , Russell James C.
Published: January 2022

Aotearoa–New Zealand has embarked on an ambitious goal: to completely eradicate key invasive mammals by 2050. This will require novel tools capable of eliminating pests on a large scale. In New Zealand, large-scale pest suppression is typically carried out using aerial application of the toxin sodium fluoroacetate (1080). However, as currently applied, this tool does not remove all individuals. A novel application method, dubbed ‘1080-to-zero’, aims to change this and reduce the abundances of target pests to zero or near-zero. One such target is black rats (Rattus rattus), an invasive species challenging to control using ground-based methods. This study monitored and compared the response of black rats to a 1080-to-zero operation and a standard suppression 1080 operation. No difference in the efficacy of rat removal was found between the two treatments. The 1080-to-zero operation did not achieve its goal of rat elimination or reduction to near-zero levels, with an estimated 1540 rats surviving across the 2200 ha treatment area. However, 1080 operations can produce variable responses, and the results observed here differ from the only other reported 1080-to-zero operation. We encourage further research into this tool, including how factors such as ecosystem type, mast fruiting and operational timing influence success.
Environmental Complexity: Additional Human Visual Contact Reduced Meat Chickens’ Fear of Humans and Physical Items Altered Pecking Behavior
Authors: Taylor Peta S. , Hemsworth Paul H. , Rault Jean-Loup
Published: January 2022

Increased environmental complexity can improve animal welfare, depending on the resources provided and use by the animal. We provided chickens either with physical items that posed no biosecurity risk and were inexpensive (balls, chains, perches and rope) (P; n = 36) or additional visual human contact (10 min daily) (HC; n = 36) compared to farm-like standard control groups (C; n = 36) with 3 pens per treatment. Additional human contact reduced fear of humans at 35 days of age, but not general fearfulness. P birds required more inductions to induce tonic immobility compared to HC and C birds at 21 days of age. However, other indicators of fear (open field test and plasma corticosterone concentration) did not significantly differ. P birds favored the woodblock for resting, and the perch but preferred to sit underneath the perch rather than on top. When pecking items were not provided, C and HC chickens redirected their pecking behavior toward the litter. Overall, there was little evidence that our physical items improved the chickens’ behavioral time budget, fear, physiological stress or production. Additional human contact should be investigated in large scale experiments to ensure its effectiveness to reduce fear of humans on farm.
Antimicrobial Resistance and Community Pharmacists’ Perspective in Thailand: A Mixed Methods Survey Using Appreciative Inquiry Theory
Authors: Netthong Rojjares , Kane Ros , Ahmadi Keivan
Published: January 2022

Global action plans to tackle antimicrobial resistance (AMR) are the subject of ongoing discussion between experts. Community pharmacists have a professional responsibility to tackle AMR. This study aimed to evaluate the knowledge of antibiotic resistance and attitudes to promoting Antibiotic Smart Use (ASU) amongst part and full-time practicing community pharmacists across Thailand. An online mixed-method survey applying Appreciative Inquiry theory was validated and conducted in 2020. Non-probability sampling was used, with online survey dissemination via social networks. A total of 387 community pharmacists located in 59 out 77 provinces seemed knowledgeable about antimicrobial resistance (mean score = 82.69%) and had acceptable attitudes towards antibiotic prescribing practices and antimicrobial stewardship (mean score = 73.12%). Less than 13% of pharmacists had postgraduate degrees. Postgraduate education, training clerkship, preceptors, and antibiotic stewardship training positively affected their attitudes. The community pharmacists proposed solutions based on the Appreciative Inquiry theory to promote ASU practices. Among these were educational programmes consisting of professional conduct, social responsibility and business administration knowledge, up-to-date legislation, and substitutional strategies to compensate business income losses.
Phytogenotypic Anthocyanin Profiles and Antioxidant Activity Variation in Fruit Samples of the American Cranberry (Vaccinium macrocarpon Aiton)
Authors: Urbstaite Rima , Raudone Lina , Janulis Valdimaras
Published: January 2022

In this study, we conducted an analysis of the qualitative and quantitative composition of anthocyanins and anthocyanidins in different cultivars and genetic clones of American cranberries grown in Lithuanian climatic conditions. Four anthocyanin compounds predominated in fruit samples of American cranberry cultivars: cyanidin-3-galactoside, cyanidin-3-arabinoside, peonidin-3-galactoside, and peonidin-3-arabinoside. They accounted for 91.66 ± 2.79% of the total amount of the identified anthocyanins. The total anthocyanin content detected via the pH differential method was found to be by about 1.6 times lower than that detected via the UPLC method. Hierarchical cluster analysis and principal component analysis showed that the ‘Woolman’ cultivar distinguished from other cranberry cultivars in that its samples contained two times the average total amount of anthocyanins (8.13 ± 0.09 mg/g). The group of American cranberry cultivars ‘Howes’, ‘Le Munyon’, and ‘BL-8’ was found to have higher than average levels of anthocyanidin galactosides (means 3.536 ± 0.05 mg/g), anthocyanidins (means 0.319 ± 0.01 mg/g), and total anthocyanins (means 6.549 ± 0.09 mg/g). The evaluation of the antioxidant effect of cranberry fruit sample extracts showed that the greatest radical scavenging activity of the cranberry fruit extracts was determined in the fruit samples of ‘Woolman’ (849.75 ± 10.88 µmol TE/g) and the greatest reducing activity was determined in ‘Le Munyon’ (528.05 ± 12.16 µmol TE/g). The study showed a correlation between the total anthocyanin content and the antiradical and reductive activity of the extracts in vitro (respectively, R = 0.635 and R = 0.507, p < 0.05).
Ellagic Acid Alleviates Oxidative Stress by Mediating Nrf2 Signaling Pathways and Protects against Paraquat-Induced Intestinal Injury in Piglets
Authors: Xiao Yuxin , Huang Rui , Wang Nan , Deng Yuankun , Tan Bie , Yin Yulong , Qi Ming , Wang Jing
Published: January 2022

The gastrointestinal tract is a key source of superoxide so as to be one of the most vulnerable to oxidative stress damage. Ellagic acid (EA), a polyphenol displays widely biological activities owing to its strong antioxidant properties. Here, we investigated the protective benefits of EA on oxidative stress and intestinal barrier injury in paraquet (PQ)-challenged piglets. A total of 40 weaned piglets were randomly divided into five groups: Control, PQ, 0.005% EA-PQ, 0.01% EA-PQ, and 0.02% EA-PQ. Piglets were intraperitoneally injected with 4 mg/kg (BW) PQ or saline on d-18, and sacrificed on d-21 of experiment. EA treatments eliminated growth-check induced by PQ and increased serum superoxide dismutase (SOD) activity but decreased serum malondialdehyde (MDA) level as compared to PQ group. EA supplementation promoted Nrf2 nuclear translocation and enhanced heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQO1) protein abundances of small intestinal mucosa. Additionally, EA improved PQ-induced crypt deepening, goblet cells loss, and villi morphological damage. Consistently, EA increased tight junction protein expression as was evident from the decreased serum diamine oxidase (DAO) levels. EA could ameliorate the PQ-induced oxidative stress and intestinal damage through mediating Nrf2 signaling pathway. Intake of EA-rich food might prevent oxidative stress-mediated gut diseases.
Recognition of mRNA N4 Acetylcytidine (ac4C) by Using Non-Deep vs. Deep Learning
Authors: Iqbal Muhammad Shahid , Abbasi Rashid , Bin Heyat Md Belal , Akhtar Faijan , Abdelgeliel Asmaa Sayed , Albogami Sarah , Fayad Eman , Iqbal Muhammad Atif
Published: January 2022

Deep learning models have been successfully applied in a wide range of fields. The creation of a deep learning framework for analyzing high-performance sequence data have piqued the research community’s interest. N4 acetylcytidine (ac4C) is a post-transcriptional modification in mRNA, is an mRNA component that plays an important role in mRNA stability control and translation. The ac4C method of mRNA changes is still not simple, time consuming, or cost effective for conventional laboratory experiments. As a result, we developed DL-ac4C, a CNN-based deep learning model for ac4C recognition. In the alternative scenario, the model families are well-suited to working in large datasets with a large number of available samples, especially in biological domains. In this study, the DL-ac4C method (deep learning) is compared to non-deep learning (machine learning) methods, regression, and support vector machine. The results show that DL-ac4C is more advanced than previously used approaches. The proposed model improves the accuracy recall area by 9.6 percent and 9.8 percent, respectively, for cross-validation and independent tests. More nuanced methods of incorporating prior bio-logical knowledge into the estimation procedure of deep learning models are required to achieve better results in terms of predictive efficiency and cost-effectiveness. Based on an experiment’s acetylated dataset, the DL-ac4C sequence-based predictor for acetylation sites in mRNA can predict whether query sequences have potential acetylation motifs.
Effect of Cold Plasma Treatment on the Packaging Properties of Biopolymer-Based Films: A Review
Authors: Hoque Monjurul , McDonagh Ciara , Tiwari Brijesh K. , Kerry Joseph P. , Pathania Shivani
Published: January 2022

Biopolymers, like polysaccharides and proteins, are sustainable and green materials with excellent film-forming potential. Bio-based films have gained a lot of attention and are believed to be an alternative to plastics in next-generation food packaging. Compared to conventional plastics, biopolymers inherently have certain limitations like hydrophilicity, poor thermo-mechanical, and barrier properties. Therefore, the modification of biopolymers or their films provide an opportunity to develop packaging materials with desired characteristics. Among different modification approaches, the application of cold plasma has been a very efficient technology to enhance the functionality and interfacial characteristics of biopolymers. Cold plasma is biocompatible, shows uniformity in treatment, and is suitable for heat-sensitive components. This review provides information on different plasma generating equipment used for the modification of films and critically analyses the impact of cold plasma on packaging properties of films prepared from protein, polysaccharides, and their combinations. Most studies to date have shown that plasma treatment effectively enhances surface characteristics, mechanical, and thermal properties, while its impact on the improvement of barrier properties is limited. Plasma treatment increases surface roughness that enables surface adhesion, ink printability, and reduces the contact angle. Plasma-treated films loaded with antimicrobial compounds demonstrate strong antimicrobial efficacy, mainly due to the increase in their diffusion rate and the non-thermal nature of cold plasma that protects the functionality of bioactive compounds. This review also elaborates on the existing challenges and future needs. Overall, it can be concluded that the application of cold plasma is an effective strategy to modify the inherent limitations of biopolymer-based packaging materials for food packaging applications.
Auxin Response Factors Are Ubiquitous in Plant Growth and Development, and Involved in Crosstalk between Plant Hormones: A Review
Authors: Kou Xiaohong , Zhao Xiaoyang , Wu Bingda , Wang Chao , Wu Caie , Yang Sen , Zhou Jiaqian , Xue Zhaohui
Published: January 2022

Auxin response factors (ARFs) are an important family of transcription factors involved in the exertion of auxin in plants and play a key role in regulating the growth and development of plant nutritional and reproductive organs such as roots, stems, leaves, flowers, fruits, and seeds. Foods of plant origin occupy an important place in the nutritional structure of the human diet, and the main edible parts of different plants vary. In this paper, we review recent research reports on ARFs and summarize its role in the regulation of leaf, flower, root, and fruit growth, as well as other important life activities. We also present the challenges and opportunities that ARFs will present in the future. It will be important to deepen our understanding of the mechanisms by which ARFs interact with other proteins or genes. In addition, it is worth considering that more technical tools should be put into the study of ARFs and that the research should be oriented towards solving practical problems. In the future, it is expected that the nutrition and function of plant-derived foods can be improved through gene editing and other means.
Development and Experimental Assessment of a Model for the Material Deposition by Laser-Induced Forward Transfer
Authors: Paris Grigori , Bierbaum Dominik , Paris Michael , Mager Dario , Loeffler Felix F.
Published: January 2022

The potential to deposit minute amounts of material from a donor to an acceptor substrate at precise locations makes laser-induced forward transfer (LIFT) a frequently used tool within different research fields, such as materials science and biotechnology. While many different types of LIFT exist, each specialized LIFT application is based on a different underlying transfer mechanism, which affects the to-be-transferred materials in different ways. Thus, a characterization of these mechanisms is necessary to understand their limitations. The most common investigative methods are high-speed imaging and numerical modeling. However, neither of these can, to date, quantify the material deposition. Here, analytical solutions are derived for the contact-based material deposition by LIFT, which are based on a previously observed equilibrium state. Moreover, an analytical solution for the previously unrecognized ejection-based material deposition is proposed, which is detectable by introducing a distance between the donor and acceptor substrates. This secondary mechanism is particularly relevant in large scale production, since each deposition from a donor substrate potentially induces a local distance between the donor and acceptor substrates.
SMC-CPHD Filter with Adaptive Survival Probability for Multiple Frequency Tracking
Authors: Kim Sun Young , Kang Chang Ho , Park Chan Gook
Published: January 2022

We propose a sequential Monte Carlo-based cardinalized probability hypothesis density (SMC-CPHD) filter with adaptive survival probability for multiple frequency tracking to enhance the tracking performance. The survival probability of the particles in the filter is adjusted using the pre-designed exponential function related to the distribution of the estimated particle points. In order to ensure whether the proposed survival probability affects the stability of the filter, the error bounds in the prediction process are analyzed. Moreover, an inverse covariance intersection-based compensation method is added to enhance cardinality tracking performance by integrating two types of cardinality information from the CPHD filter and data clustering process. To evaluate the proposed method’s performance, MATLAB-based simulations are performed. As a result, the tracking performance of the multiple frequencies has been confirmed, and the accuracy of cardinality estimates are improved compared to the existing filters.
Risk Assessment of Riverine Terraces: The Case of the Chenyulan River Watershed in Nantou County, Taiwan
Authors: Lin Ji-Yuan , Chao Jen-Chih , Hsu Yung-Ming
Published: January 2022

The purpose of this study is to establish a method of hazard assessment for the river terraces along the Chenyulan River and use 40 of them as protected objects. Using a geographic information system, the researchers extracted nine parameters for such terraces. These are length to attack shore, distance away from fault, distance from river channel, number of creeks and streams with possibility of debris flows, height above stream level, average slope degree, geology, number of erosion ditches, and distance from landslide area behind. Next, the weightings identified by analytic hierarchy process analysis were used as the basis for grading the various factors affecting river terraces. Hazard assessment for the river terraces then proceeded via totaling of the potential trends of the various factors and the protected objects, as well as comparison of historical disaster conditions and satellite images. The results showed that there were 8 high-risk river terraces, 14 medium–high-risk river terraces, 14 medium–low-risk river terraces and 4 low-risk river terraces. The evaluation of the current conditions of the settlement environment through parameter weighting has a certain accuracy and reference value in reducing the disaster impact of the riverine terrace settlement.
Password-Guessing Attack-Aware Authentication Scheme Based on Chinese Remainder Theorem for 5G-Enabled Vehicular Networks
Authors: Al-Shareeda Mahmood A. , Anbar Mohammed , Manickam Selvakumar , Hasbullah Iznan H.
Published: January 2022

The new fifth-generation (5G) cellular networks dramatically improve the speed of message transmissions. Most existing authentication schemes that secure 5G communication rely heavily on the vehicle’s tamper-proof device (TPD) and roadside units (RSUs) to store the system’s master key. However, it only takes a single compromised TPD to render the whole system insecure. We propose a password-guessing attack-aware authentication scheme based on the Chinese Remainder Theorem (CRT) to secure inter-vehicle communication on 5G-enabled vehicular networks to address this issue. The trusted authorities (TAs) in the proposed scheme generate and broadcast new group keys to the vehicles assisted by CRT. Moreover, since the system’s master key does not need to be preloaded, the proposed scheme only requires realistic TPDs. The proposed scheme overcomes password-guessing attacks and guarantees top-level security for entire 5G-enabled vehicular networks. The security analysis indicates that the proposed scheme is secure against adaptive chosen-message attacks under the random oracle model and meets the security requirements of a 5G-enabled vehicular network. Since cryptographic operations based on elliptic curve cryptography are employed, the performance evaluation shows that the proposed scheme outperforms the eight existing schemes in terms of computation and communication costs.