bluuebunny/crossref_metadata_2025 huggingface dataset:
Mimicking Strategy for Protein–Protein Interaction Inhibitor Discovery by Virtual Screening
Authors: Wu Ke-Jia , Lei Pui-Man , Liu Hao , Wu Chun , Leung Chung-Hang , Ma Dik-Lung
Published: December 2019

As protein–protein interactions (PPIs) are highly involved in most cellular processes, the discovery of PPI inhibitors that mimic the structure of the natural protein partners is a promising strategy toward the discovery of PPI inhibitors. In this review, we discuss recent advances in the application of virtual screening for identifying mimics of protein partners. The classification and function of the mimicking protein partner inhibitor discovery by virtual screening are described. We anticipate that this review would be of interest to medicinal chemists and chemical biologists working in the field of protein–protein interaction inhibitors or probes.
Rheological, Thermal, Superficial, and Morphological Properties of Thermoplastic Achira Starch Modified with Lactic Acid and Oleic Acid
Authors: Caicedo Carolina , Aguirre Loredo Rocío Yaneli , Fonseca García Abril , Ossa Omar Hernán , Vázquez Arce Aldo , Calambás Pulgarin Heidy Lorena , Ávila Torres Yenny
Published: December 2019

The modification of achira starch a thermoplastic biopolymer is shown. Glycerol and sorbitol, common plasticizers, were used in the molten state with organic acids such as oleic acid and lactic acid obtaining thermodynamically more stable products. The proportion of starch:plasticizer was 70:30, and the acid agent was added in portions from 3%, 6%, and 9% by weight. These mixtures were obtained in a torque rheometer for 10 min at 130 °C. The lactic acid managed to efficiently promote the gelatinization process by increasing the available polar sites towards the surface of the material; as a result, there were lower values in the contact angle, these results were corroborated with the analysis performed by differential scanning calorimetry and X-ray diffraction. The results derived from oscillatory rheological analysis had a viscous behavior in the thermoplastic starch samples and with the presence of acids; this behavior favors the transitions from viscous to elastic. The mixture of sorbitol or glycerol with lactic acid promoted lower values of the loss module, the storage module, and the complex viscosity, which means lower residual energy in the transition of the viscous state to the elastic state; this allows the compounds to be scaled to conventional polymer transformation processes.
Routine Management of Microalgae Using Autofluorescence from Chlorophyll
Authors: Takahashi Toshiyuki
Published: December 2019

From a high-potential biomass perspective, microalgae have recently attracted considerable attention due to their extensive application in many areas. Although studies searching for algal species with extensive application potential are ongoing, technical development for their assessment and maintenance of quality in culture are also critical and inescapable challenges. Considering the sensitivity of microalgae to environmental changes, management of algal quality is one of the top priorities for industrial applications. Helping substitute for conventional methods such as manual hemocytometry, turbidity, and spectrophotometry, this review presents an image-based, automated cell counter with a fluorescence filter to measure chlorophyll autofluorescence emitted by algae. Capturing chlorophyll-bearing cells selectively, the device accomplished precise qualification of algal numbers. The results for cell density using the device with fluorescence detection were almost identical to those obtained using hemocytometry. The automated functions of the device allow operators to reduce working hours, for not only cell density analysis but simultaneous multiparametric analysis such as cell size and algal status based on chlorophyll integrity. The automated device boldly supports further development of algal application and might contribute to opening up more avenues in the microalgal industry.
The Role of c-Met as a Biomarker and Player in Innate and Acquired Resistance in Non-Small-Cell Lung Cancer: Two New Mutations Warrant Further Studies
Authors: Van Der Steen Nele , Zwaenepoel Karen , Mazzaschi Giulia , A. Luirink Rosa , P. Geerke Daan , Op de Beeck Ken , Hermans Christophe , Tiseo Marcello , Van Schil Paul , Lardon Filip , Germonpré Paul , Rolfo Christian , Giovannetti Elisa , J. Peters Godefridus , Pauwels Patrick
Published: December 2019

The c-Met receptor is a therapeutically actionable target in non-small-cell lung cancer (NSCLC), with one approved drug and several agents in development. Most suitable biomarkers for patient selection include c-Met amplification and exon-14 skipping. Our retrospective study focused on the frequency of different c-Met aberrations (overexpression, amplification and mutations) in 153 primary, therapy-naïve resection samples and their paired metastases, from Biobank@UZA. Furthermore, we determined the correlation of c-Met expression with clinicopathological factors, Epidermal Growth Factor Receptor (EGFR)-status and TP53 mutations. Our results showed that c-Met expression levels in primary tumors were comparable to their respective metastases. Five different mutations were detected by deep sequencing: three (E168D, S203T, N375S) previously described and two never reported (I333T, G783E). I333T, a new mutation in the Sema(phorin) domain of c-Met, might influence the binding of antibodies targeting the HGF-binding domain, potentially causing innate resistance. E168D and S203T mutations showed a trend towards a correlation with high c-Met expression (p = 0.058). We found a significant correlation between c-MET expression, EGFR expression (p = 0.010) and EGFR mutations (p = 0.013), as well as a trend (p = 0.057) with regards to TP53 mutant activity. In conclusion this study demonstrated a strong correlation between EGFR mutations, TP53 and c-Met expression in therapy-naïve primary resection samples. Moreover, we found two new c-Met mutations that warrant further studies.
In Situ Real-Time Tracing of Organophosphorus Pesticides in Apples by Solid-Phase Microextraction with Developed Sampling-Rate Calibration
Authors: Zhang Xiao-Fan , Zhao Li-Li , Huang Ming-Quan , Li Xiu-Juan , Pan Si-Yi
Published: December 2019

An in situ tracing study based on solid-phase microextraction (SPME) was conducted to investigate the uptake and elimination of organophosphorus pesticides in apples. A matrix-compatible polydimethylsiloxane/poly(styrene-co-divinylbenzene)/polydimethylsiloxane fiber was produced to meet the needs of in situ sampling. The fiber had high extraction ability, good sensitivity and accuracy with respect to the analytes in apple pulp, and could be used 85 times. Although the sampling rate was changing over time, quantification was still achieved by the sampling rate calibration method. Some factors that affect its applicability were studied. The limits of detection were 0.18 ng/g for diazinon and 0.20 ng/g for chlorpyrifos, rather lower than the maximum residue limits of the National Food Safety Standard of China (GB 2763-2016) and the European Commission (Reg.(EU) No 834/2013, 2018/686). The accuracy of in situ SPME quantification was verified by comparing with the results obtained by the traditional liquid–liquid extraction method. In this work, the in situ sampling method is developed using apples, diazinon, and chlorpyrifos as a model system; however, this method can be used for in vivo analysis of fruits and vegetables for nutrition and safety monitoring.
Towards Covalent Photosensitizer-Polyoxometalate Dyads-Bipyridyl-Functionalized Polyoxometalates and Their Transition Metal Complexes
Authors: Winter Andreas , Endres Patrick , Schröter Erik , Jäger Michael , Görls Helmar , Neumann Christof , Turchanin Andrey , Schubert Ulrich S.
Published: December 2019

A triol-functionalized 2,2′-bipyridine (bpy) derivative has been synthesized and used for the tris-alkoxylation of polyoxometalate (POM) precursors. The resultant POM-bpy conjugates of the Wells–Dawson- and Anderson-type feature a C–C bond as a linkage between the POM and bpy fragments. This structural motif is expected to increase the hydrolytic stability of the compounds. This is of particular relevance with respect to the application of POM-bpy metal complexes, as photocatalysts, in the hydrogen-evolution reaction (HER) in an aqueous environment. Accordingly, Rh(III) and Ir(III) complexes of the POM-bpy ligands have been prepared and characterized. These catalyst-photosensitizer dyads have been analyzed with respect to their electrochemical and photophysical properties. Cyclic and square-wave voltammetry, as well as UV/vis absorption and emission spectroscopy, indicated a negligible electronic interaction of the POM and metal-complex subunits in the ground state. However, emission–quenching experiments suggested an efficient intramolecular electron-transfer process from the photo-excited metal centers to the POM units to account for the non-emissive nature of the dyads (thus, suggesting a strong interaction of the subunits in the excited state). In-depth photophysical investigations, as well as a functional characterization, i.e., the applicability in the HER reaction, are currently ongoing.
Synthesis and Structure of Methylsulfanyl Derivatives of Nickel Bis(Dicarbollide)
Authors: Anufriev Sergey A. , Suponitsky Kyrill Yu. , Filippov Oleg A. , Sivaev Igor B.
Published: December 2019

Symmetrically and unsymmetrically substituted methylsulfanyl derivatives of nickel(III) bis(dicarbollide) (Bu4N)[8,8′-(MeS)2-3,3′-Ni(1,2-C2B9H10)2], (Bu4N)[4,4′-(MeS)2-3,3′-Ni(1,2-C2B9H10)2], and (Bu4N)[4,7′-(MeS)2-3,3′-Ni(1,2-C2B9H10)2] were synthesized, starting from [Ni(acac)2]3 and the corresponding methylsulfanyl derivatives of nido-carborane (Bu4N)[10-MeS-7,8-C2B9H11] and (Bu4N)[10-MeS-7,8-C2B9H11]. Structures of the synthesized metallacarboranes were studied by single-crystal X-ray diffraction and quantum chemical calculations. The symmetrically substituted 8,8′-isomer adopts transoid conformation stabilized by two pairs of intramolecular C–H···S hydrogen bonds between the dicarbollide ligands. The unsymmetrically substituted 4,7′-isomer adopts gauche conformation, which is stabilized by two nonequivalent C–H···S hydrogen bonds and one short chalcogen B–H···S bond (2.53 Å, −1.4 kcal/mol). The gauche conformation was found to be also preferred for the 4,7′-isomer.
Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
Authors: Lee Changyeol , Li Wei , Bang Sunghee , Lee Sun Joo , Kang Nam-young , Kim Soonok , Kim Tae In , Go Younghoon , Shim Sang Hee
Published: December 2019

The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2–8), two isochromanones (9, 10), one perylenequinone (11), and one benzocycloalkanone (12). Their structures were determined via 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and computational electronic circular dichroism (ECD) analysis. Compounds 3 and 11 increased pyruvate dehydrogenase (PDH) activity in AD-293 human embryonic kidney cells and significantly inhibited PDH phosphorylation. The IC50 values of 3 and 11 were 32.58 and 27.82 μM, respectively.
High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur
Authors: Weber Patrick , Pissis Cédric , Navaza Rafael , Mechaly Ariel E. , Saul Frederick , Alzari Pedro M. , Haouz Ahmed
Published: December 2019

The availability of whole-genome sequence data, made possible by significant advances in DNA sequencing technology, led to the emergence of structural genomics projects in the late 1990s. These projects not only significantly increased the number of 3D structures deposited in the Protein Data Bank in the last two decades, but also influenced present crystallographic strategies by introducing automation and high-throughput approaches in the structure-determination pipeline. Today, dedicated crystallization facilities, many of which are open to the general user community, routinely set up and track thousands of crystallization screening trials per day. Here, we review the current methods for high-throughput crystallization and procedures to obtain crystals suitable for X-ray diffraction studies, and we describe the crystallization pipeline implemented in the medium-scale crystallography platform at the Institut Pasteur (Paris) as an example.
Photoinduced Electron Transfer-Promoted Reactions Using Exciplex-Type Organic Photoredox Catalyst Directly Linking Donor and Acceptor Arenes
Authors: Yamawaki Mugen , Asano Akiko , Furutani Toshiki , Izumi Yuki , Tanaka Yosuke , Osaka Kazuyuki , Morita Toshio , Yoshimi Yasuharu
Published: December 2019

Directly linked donor and acceptor arenes, such as phenanthrene/naphthalene/biphenyl and 1,3-dicyanobenzene were found to work as photoredox catalysts in the photoreactions of indene, 2,3-dimethyl-2-butene, and 4-methoxyphenylacetic acid. The new stable organic photocatalyst forms an intramolecular exciplex (excited complex) when irradiated in a polar solvent and shows redox catalyst activity, even at low concentrations. To the best of our knowledge, this is the first example of an intramolecular exciplex working as a redox catalyst.
Chemical Composition of Essential Oil from Flower Heads of Arnica Chamissonis Less. under a Nitrogen Impact
Authors: Sugier Danuta , Olesińska Katarzyna , Sugier Piotr , Wójcik Małgorzata
Published: December 2019

Chamisso arnica (Arnica chamissonis Less.) is a valuable plant species used in the pharmaceutical industry due to the content of many pharmacologically active substances and the similarity of its chemical composition to that of Arnica montana—a medicinal plant commonly used in pharmacy and cosmetics. The similarity of the two plant species implies that chamisso arnica can be a pharmaceutical substitute for the mountain arnica, i.e., an endangered and endemic plant species in Europe. Chamisso arnica extracts exhibit anti-inflammatory and antiradical activity and possesses high antioxidant properties that might be helpful in preventing or delaying the progress of free radical dependent diseases. The attributes of A. chamissonis are mainly related to the content and chemical composition of essential oil. Therefore, the objective of this study was to characterize the chemical composition of essential oil derived from A. chamissonis flower heads under a nitrogen impact. The experiment was performed on experimental fields in mid-eastern Poland on two soil types (sandy and loamy soils). The nitrogen fertilizer was applied as ammonium sulfate (control, 30, 60, 90, and 120 kg N ha−1). Collection of flower heads was carried out in the full flowering phase, which was characterized by the highest content of essential oil. The chemical composition of essential oil was examined using GC-MS. Among the 75 ingredients of the volatile oil of chamisso arnica flower heads, alpha-pinene, cumene, p-cymene, germacrene D, spathulenol, decanal, caryophyllene oxide, beta-pinene, and benzene acetaldehyde were present at relatively high levels. Both the nitrogen application and the soil type had an effect on the oil concentration and the yield of the main constituents (alpha-pinene and germacrene D) with pharmacological value. Different levels of nitrogen application could be considered as a relevant way to modify the chemical composition and to increase the essential oil production.
Influence of Different Frying Processes on the Flavor Characteristics and Sensory Profile of Garlic Oil
Authors: Sun Jie , Sun Baoguo , Ren Fazheng , Chen Haitao , Zhang Ning , Zhang Yuyu
Published: December 2019

Fried garlic oil has been widely used in traditional Chinese cuisine and, recently, has become increasingly popular in food manufacturing. In this study, the effects of different initial and final frying temperature on the flavor characteristics and sensory profile of fried garlic oil were investigated using solvent-assisted flavor evaporation (SAFE) combined with gas chromatography–mass spectrometry (GC–MS). Results showed that the content of flavor compounds changed significantly as the frying temperature was increased. The sample that was treated at an initial temperature of 115 °C and a final temperature of 155 °C contained the highest amount of thioethers and heterocycles, mainly comprising dimethyl trisulfide, diallyl disulfide, and 2-vinyl-4H-1,2-dithiin. Partial least-squares regression elucidated the sensory attributes of fried and roasted garlic, showing a high correlation with thioethers and pyrazines. Furthermore, changes in the 2,6-dimethylpyrazine, dimethyl trisulfide, and diallyl disulfide concentrations were detected every 5 °C during the frying process (initial temperature, 115 °C; final temperature, 155 °C). Dimethyl trisulfide and diallyl disulfide concentrations showed irregular, downward trends, while 2,6-dimethylpyrazine concentration exhibited an increasing trend.
Potential Risk to Pollinators from Nanotechnology-Based Pesticides
Authors: Hooven Louisa A. , Chakrabarti Priyadarshini , Harper Bryan J. , Sagili Ramesh R. , Harper Stacey L.
Published: December 2019

The decline in populations of insect pollinators is a global concern. While multiple factors are implicated, there is uncertainty surrounding the contribution of certain groups of pesticides to losses in wild and managed bees. Nanotechnology-based pesticides (NBPs) are formulations based on multiple particle sizes and types. By packaging active ingredients in engineered particles, NBPs offer many benefits and novel functions, but may also exhibit different properties in the environment when compared with older pesticide formulations. These new properties raise questions about the environmental disposition and fate of NBPs and their exposure to pollinators. Pollinators such as honey bees have evolved structural adaptations to collect pollen, but also inadvertently gather other types of environmental particles which may accumulate in hive materials. Knowledge of the interaction between pollinators, NBPs, and other types of particles is needed to better understand their exposure to pesticides, and essential for characterizing risk from diverse environmental contaminants. The present review discusses the properties, benefits and types of nanotechnology-based pesticides, the propensity of bees to collect such particles and potential impacts on bee pollinators.
Fluorescence and Time-Delayed Lasing during Single Laser Pulse Excitation of a Pendant mm-Sized Dye Droplet
Authors: Boni Mihai , Andrei Ionut Relu , Pascu Mihail Lucian , Staicu Angela
Published: December 2019

Fluorescence and lasing emission that are produced separately in time during excitation laser pulse for an mm-sized Rhodamine 6G dye-water droplet are reported. The droplet acts as a quasi-spherical closed optical resonator and due to multiple internal reflections, the resonant amplified emission is delayed with respect to fluorescence emission. Measurements of the temporal evolution of the droplet’s emission were performed by varying the signal acquisition gate width and gate delay with respect to the pumping pulse. The droplet emission spectra are structured in two bands which appear one after the other in time: first, the fluorescence emission band which follows pumping laser pulse time shape and then a second band, the lasing band, placed at shorter wavelengths and formed in time after the peak of the pumping laser pulse intensity, on the pulse tail. The lasing threshold pumping intensity is much lower than those for typical dye lasers.
Mercury Content in Central and Southern Adriatic Sea Sediments in Relation to Seafloor Geochemistry and Sedimentology
Authors: Droghini Elisa , Annibaldi Anna , Prezioso Emanuela , Tramontana Mario , Frapiccini Emanuela , De Marco Rocco , Illuminati Silvia , Truzzi Cristina , Spagnoli Federico
Published: December 2019

Mercury contents were determined in surface sediments from the Central and Southern Adriatic Sea to gain insight into the processes, factors, and variables affecting its distribution. Mercury concentration was measured by thermal decomposition amalgamation atomic absorption spectrometry in samples collected by box-corer from Ancona to Santa Maria di Leuca during the CNR-PERTRE cruise (16/9-4/10/2016). Sediments were also evaluated for chemical-physical parameters (pH, Eh), biogeochemical composition (total carbon, inorganic carbon, total organic carbon, organic matter) and grain size. The average mercury concentration in the Adriatic Sea sediment was 0.053 mg/Kg (d.w.), range 0.011–0.12 mg/Kg (d.w.). Mercury content was mainly affected by grain size and organic matter (OM) distribution, whereas anthropic factors exerted a limited influence. Concentrations followed the distribution of sediment types (clay > silt > sand) due to Adriatic Sea hydrodynamics and were well below the regulatory limits in all samples.
NH3-Sensing Mechanism Using Surface Acoustic Wave Sensor with AlO(OH) Film
Authors: Xu Xiaofeng , Zu Xiaotao , Ao Dongyi , Yu Jingxia , Xiang Xia , Xie Wanfeng , Tang Yongliang , Li Sean , Fu Yongqing
Published: December 2019

In this study, AlO(OH) (boehmite) film was deposited onto a surface acoustic wave (SAW) resonator using a combined sol-gel and spin-coating technology, and prepared and used as a sensitive layer for a high-performance ammonia sensor. The prepared AlO(OH) film has a mesoporous structure and a good affinity to NH3 (ammonia gas) molecules, and thus can selectively adsorb and react with NH3. When exposed to ammonia gases, the SAW sensor shows an initial positive response of the frequency shift, and then a slight decrease of the frequency responses. The sensing mechanism of the NH3 sensor is based on the competition between mass-loading and elastic-loading effects. The sensor operated at room temperature shows a positive response of 1540 Hz to 10 ppm NH3, with excellent sensitivity, selectivity and stability.
A Novel Design Strategy for Suppressing Efficiency Roll-Off of Blue Thermally Activated Delayed Fluorescence Molecules through Donor–Acceptor Interlocking by C–C Bonds
Authors: Kwon Tae Hui , Jeon Soon Ok , Numata Masaki , Lee Hasup , Chung Yeon Sook , Kim Jong Soo , Ihn Soo-Ghang , Sim Myungsun , Kim Sunghan , Kim Byeong Moon
Published: December 2019

The short material lifetime of thermally activated delayed fluorescence (TADF) technology is a major obstacle to the development of economically feasible, highly efficient, and durable devices for commercial applications. TADF devices are also hampered by insufficient operational stability. In this paper, we report the design, synthesis, and evaluation of new TADF molecules possessing a sterically twisted skeleton by interlocking donor and acceptor moieties through a C–C bond. Compared to C–N-bond TADF molecules, such as CPT2, the C–C-bond TADF molecules showed a large dihedral angle increase by more than 30 times and a singlet–triplet energy-gap decrease to less than 0.22 eV because of the steric hindrance caused by the direct C–C bond connection. With the introduction of a dibenzofuran core structure, devices comprising BMK-T317 and BMK-T318 exhibited a magnificent display performance, especially their external quantum efficiencies, which were as high as 19.9% and 18.8%, respectively. Moreover, the efficiency roll-off of BMK-T318 improved significantly (26.7%). These results indicate that stability of the material can be expected through the reduction of their singlet–triplet splitting and the precise adjustment of dihedral angles between the donor–acceptor skeletons.
Percutaneous Endoscopic Gastrostomy versus Nasogastric Tube Feeding: Oropharyngeal Dysphagia Increases Risk for Pneumonia Requiring Hospital Admission
Authors: Chang Wei-Kuo , Huang Hsin-Hung , Lin Hsuan-Hwai , Tsai Chen-Liang
Published: December 2019

Background: Aspiration pneumonia is the most common cause of death in patients with percutaneous endoscopic gastrostomy (PEG) and nasogastric tube (NGT) feeding. This study aimed to compare PEG versus NGT feeding regarding the risk of pneumonia, according to the severity of pooling secretions in the pharyngolaryngeal region. Methods: Patients were stratified by endoscopic observation of the pooling secretions in the pharyngolaryngeal region: control group (<25% pooling secretions filling the pyriform sinus), pharyngeal group (25–100% pooling secretions filling the pyriform sinus), and laryngeal group (pooling secretions entering the laryngeal vestibule). Demographic data, swallowing level scale score, and pneumonia requiring hospital admission were recorded. Results: Patients with NGT (n = 97) had a significantly higher incidence of pneumonia (episodes/person-years) than those patients with PEG (n = 130) in the pharyngeal group (3.6 ± 1.0 vs. 2.3 ± 2.1, P < 0.001) and the laryngeal group (3.8 ± 0.5 vs. 2.3 ± 2.2 vs, P < 0.001). The risk of pneumonia was significantly higher in patients with NGT than in patients with PEG (adjusted hazard ratio = 2.85, 95% CI: 1.46–4.98, P < 0.001). Cumulative proportion of pneumonia was significantly higher in patients with NGT than with PEG for patients when combining the two groups (pharyngeal + laryngeal groups) (P = 0.035). Conclusion: PEG is a better choice than NGT feeding due to the decrease in risk of pneumonia requiring hospital admission, particularly in patients with abnormal amounts of pooling secretions accumulation in the pyriform sinus or leak into the laryngeal vestibule.
Giardia intestinalis and Fructose Malabsorption: A Frequent Association
Authors: Trelis María , Taroncher-Ferrer Silvia , Gozalbo Mónica , Ortiz Vicente , Soriano José M. , Osuna Antonio , Merino-Torres Juan F.
Published: December 2019

Nowadays, scientific studies are emerging on the possible etiological role of intestinal parasites in functional digestive disorders. Our study was carried out with healthy individuals (control group; n = 82) and symptomatic patients with lactose or fructose malabsorption, including positive (malabsorbers; n = 213) and negative (absorbers; n = 56) breath test, being analyzed for the presence of intestinal parasites. A high parasitic prevalence was observed in malabsorbers (41.8%), exclusively due to single-cell eukaryotes but not helminths. Giardia intestinalis was the predominant parasite in cases of abnormal absorption (26.5%), significantly associated with fructose malabsorption and doubling the probability of developing this pathology. Within controls, Blastocystis sp. (13.4%) was almost the only parasite, being the second among patients (12.6%), and Cryptosporidium parvum, the last species of clinical relevance, was detected exclusively in two malabsorbers (0.9%). The consumption of ecological food and professions with direct contact with humans arose as risk factors of parasitism. A diagnosis of carbohydrate malabsorption in adulthood is the starting point, making the search for the primary cause necessary. Accurate parasitological diagnosis should be considered another tool in the clinical routine for patients with recurrent symptoms, since their condition may be reversible with adequate therapeutic intervention.
Morphology and Multi-Gene Phylogeny Reveal Pestalotiopsis pinicola sp. nov. and a New Host Record of Cladosporium anthropophilum from Edible Pine (Pinus armandii) Seeds in Yunnan Province, China
Authors: Tibpromma Saowaluck , Mortimer Peter E. , Karunarathna Samantha C. , Zhan Fangdong , Xu Jianchu , Promputtha Itthayakorn , Yan Kai
Published: December 2019

This study contributes new knowledge on the diversity of conidial fungi in edible pine (Pinus armandii) seeds found in Yunnan Province, China and emphasizes the importance of edible seed products to ensure food safety standards. We isolated two fungal species, one on the pine seed coat and the other on the endosperm of the pine seed. The two fungal species were identified as Pestalotiopsis pinicola sp. nov. and a new host record Cladosporium anthropophilum. Characteristic morphological features of Pestalotiopsis pinicola were used alongside results from multi-gene phylogenetic analysis to distinguish it from currently known species within the genus. Cladosporium anthropophilum was identified as a new host record based on morphological features and phylogenetic analysis. In addition, detailed descriptions, scanned electron microscopy morphology, illustrations, and phylogenetic trees are provided to show the placement of these species.
Optimize, Modulate, and Scale-up Resveratrol and Resveratrol Dimers Bioproduction in Vitis labrusca L. Cell Suspension from Flasks to 20 L Bioreactor
Authors: Lambert Carole , Lemaire Julien , Auger Hélène , Guilleret Arnaud , Reynaud Romain , Clément Christophe , Courot Eric , Taidi Behnam
Published: December 2019

Resveratrol and its oligomers are biologically active compounds. This work brings new insights for the bioproduction of trans-resveratrol with three dimers, pallidol, trans-ε-viniferin, and trans-δ-viniferin, in cell suspension of Vitis labrusca. Conditions of elicitation by methyl jasmonate were optimized for the production of stilbenes using statistical design of experiment. Bio-production of stilbenes was scaled-up to 5 L and in these conditions, trans-resveratrol concentrations reached 237 mg/L, and for pallidol 114 mg/L. The comparison of different elicitation modes (different elicitors, combination with cyclodextrins or adsorbent resin) allowed to reach particularly high concentrations of target molecules: Resveratrol 6.14 g/L, pallidol 0.90 g/L, δ-viniferin 0.54 g/L, and ε-viniferin 0.50 g/L. Scale-up to 20 L-stirring-bioreactor gave similar growth rates to those observed in shake flask culture, with a high production of resveratrol (4.23 g/L) and δ-viniferin (0.76 g/L). This work provides new strategies for the production of stilbenes in plant cell suspension for biological and commercial evaluation.
Bacterium-Mediated RNA Interference: Potential Application in Plant Protection
Authors: Goodfellow Simon , Zhang Daai , Wang Ming-Bo , Zhang Ren
Published: December 2019

RNAi has emerged as a promising tool for targeting agricultural pests and pathogens and could provide an environmentally friendly alternative to traditional means of control. However, the deployment of this technology is still limited by a lack of suitable exogenous- or externally applied delivery mechanisms. Numerous means of overcoming this limitation are being explored. One such method, bacterium-mediated RNA interference, or bmRNAi, has been explored in other systems and shows great potential for application to agriculture. Here, we review the current state of bmRNAi, examine the technical limitations and possible improvements, and discuss its potential applications in crop protection.
Preparation of Boron Nitride-Coated Carbon Fibers and Synergistic Improvement of Thermal Conductivity in Their Polypropylene-Matrix Composites
Authors: Badakhsh Arash , Han Woong , Jung Sang-Chul , An Kay-Hyeok , Kim Byung-Joo
Published: December 2019

The purpose of this study is to prepare boron nitride (BN)-coated carbon fibers (CF) and to investigate the properties of as-prepared fibers as well as the effect of coating on their respective polymer–matrix composites. A sequence of solution dipping and heat treatment was performed to blanket the CFs with a BN microlayer. The CFs were first dipped in a boric acid solution and then annealed in an ammonia–nitrogen mixed gas atmosphere for nitriding. The presence of BN on the CF surface was confirmed using FTIR, XPS, and SEM analyses. Polypropylene was reinforced with BN–CFs as the first filler and graphite flake as the secondary filler. The composite characterization indicates approximately 60% improvement in through-plane thermal conductivity and about 700% increase in the electrical resistivity of samples containing BN-CFs at 20 phr. An increase of two orders of magnitude in the electrical resistivity of BN–CF monofilaments was also observed.
Green Flexible Polyurethane Foam as a Potent Support for Fe-Si Adsorbent
Authors: Ahmad Afiqah , Jamil Siti Nurul Ain Md. , Shean Yaw Choong Thomas , Abdullah Abdul Halim , Mastuli Mohd Sufri , Othman Nurhanisah , Jiman NurNazurah
Published: December 2019

This paper describes the preparation, characterisation, and potential application of flexible palm oil-based polyurethane foam (PUF) as a support for iron-silica (Fe-Si) adsorbent. Fe-Si/polyurethane composite (Fe-Si/PUC) was prepared by impregnating Fe-Si adsorbent onto the surface of PUF by using a novel immersion-drying method. Morphological analysis of Fe-Si/PUC proved that Fe-Si was successfully impregnated onto the surface of PUF. Compression test and thermogravimetric analysis were carried out to determine the flexibility and thermal stability of Fe-Si/PUC, respectively. The Fe-Si/PUC removed 90.0% of 10 ppm methylene blue (MB) from aqueous solution in 60 min. The reusability study showed that Fe-Si/PUC removed 55.9% of MB on the seventh cycle. Hence, the synthesis of Fe-Si/PUC opens up a new path of implementing palm oil-based PUF to assist in the recovery of an adsorbent for environmental clean-up. The mechanism of physical interaction during the impregnation of Fe-Si adsorbent onto PUF was proposed in this paper.
Waste PET as a Reactant for Lanthanide MOF Synthesis and Application in Sensing of Picric Acid
Authors: Zhang Feng , Chen Shuyi , Nie Shengqiang , Luo Jun , Lin Shaomin , Wang Yi , Yang Huan
Published: December 2019

In this study, a lanthanide metal organic framework based on the ligand of terephthalic acid derived from waste polyethylene terephthalate (PET) bottles was designed and synthesized. The structure and morphology of the Tb-BDC was investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The Tb-BDC displays a high selectivity and sensitivity towards picric acid (TNP). The luminescence intensities exhibit a linear relation, with a concentration of TNP over the range of 1 × 10−5–1 × 10−4 M, with a limit of detection of 1 × 10−5 M. The sensing mechanism is also discussed. This is the first time that waste PET materials have been used as the starting precursor of terephthalic acid (BDC) for the fabrication of lanthanide MOF (metal organic framework), which is applied in sensing TNP.
Improved Processing and Properties for Polyphenylene Oxide Modified by Diallyl Orthophthalate Prepolymer
Authors: Wang Honghua , Mei Qilin , Ding Yujie , Huang Zhixiong , Shi Minxian
Published: December 2019

Diallyl orthophthalate (DAOP) prepolymer was investigated as a reactive plasticizer to improve the processability of thermoplastics. The rheology of blends of DAOP prepolymer initiated by 2,3-dimethyl-2,3-diphenylbutane (DMDPB) and polyphenylene oxide (PPO) was monitored during the curing process, and their thermal properties and morphology in separated phases were also studied. Differential scanning calorimetry (DSC) results showed that the cure degree of the reactively plasticized DAOP prepolymer was reduced with increasing PPO due to the dilution effect. The increasing amount of the DAOP prepolymer led to a gradual decrease in the viscosity of the blends and the rheology behavior was consistent with the chemical gelation of DAOP prepolymer in blends. This indicated that the addition of the DAOP prepolymer effectively improved processability. The phase separation occurring during curing of the blend and the transition from the static to dynamic mode significantly influences the development of the morphology of the blend corresponding to limited evolution of the conversion around the gel point.
The Effects of Strain Rates on Mechanical Properties and Failure Behavior of Long Glass Fiber Reinforced Thermoplastic Composites
Authors: Cui Junjia , Wang Shaoluo , Wang Shuhao , Li Guangyao , Wang Peilin , Liang Chengsong
Published: December 2019

Long glass fiber reinforced thermoplastic composites have been increasingly used in automotive parts due to their excellent mechanical properties and recyclability. However, the effects of strain rates on the mechanical properties and failure mechanisms of long glass fiber reinforced polypropylene composites (LGFRPPs) have not been studied systematically. In this study, the effects of strain rates (from 0.001 s−1 to 400 s−1) on the mechanical properties and failure mechanism of LGFRPPs were investigated. The results showed that ultimate strength and fracture strain of the LGFRPPs increased obviously, whereas the stiffness remained essentially unchanged with the strain rates from low to high. The micro-failure modes mainly consisted of fibers pulled out, fiber breakage, interfacial debonding, matrix cracking, and ductile to brittle (ductile pulling of fibrils/micro-fibrils) fracture behavior of the matrix. As the strain rates increased, the interfacial bonding properties of LGFRPPs increased, resulting in a gradual increase of fiber breakage at the fracture surface of the specimen and the gradual decrease of pull-out. In this process, more failure energy was absorbed, thus, the ultimate strength and fracture strain of LGFRPPs were improved.
Memory and History: The Overcoming of Traditional Theodicy in Levinas and Metz
Authors: Losada-Sierra Manuel
Published: December 2019

Grappling with the marginalization of the marginal in Western thinking, this paper sets up a dialogue between Emmanuel Levinas’s philosophy and Johann Baptist Metz’s political theology in order to learn from their thoughts on the suffering of victims. For both Levinas and Metz, the idea of theodicy as an explanation of suffering is linked to the ontological conception of time and history, and therefore useless and unjustifiable by nature. The essential question of this research is how to give meaning to the concrete suffering of humanity in order to redeem history from the concept of an evolutionary progress which limits the possibility of hearing the cries of the victims of history. This article will show how Levinas’s and Metz´s rejection of traditional theodicy is closely related to the concepts of memory and history and, therefore, the paper will demonstrate how traditional theodicy becomes for both thinkers an ethical theodicy. Consequently, the ethical account of theodicy replaces the attempt to negotiate the goodness and power of God with the pain of human beings. From this perspective, ethics is shaped by a response to the cry of victims which summons the subject to understand freedom as limited and subordinated to ethical responsibility. In responding to suffering, philosophy and theology can meet beyond idealism and dogmatism.
‘Smell Your Sheep, Shepherd’: What Does It Mean to Be Catholic for the Dalit?
Authors: Robinson Rowena
Published: December 2019

The anthropology of Christianity has emerged as an exciting field in the last decade or so. Themes of interest for us in India and South Asia in general include issues of caste, conversion and belief, the ideas of sin and morality, individualism, and the like. As part of this growing field, the issue of belief in particular has gained considerable importance. It has been argued that the strict reliance on belief is obstructive and counterproductive for the understanding of non-Western Christianity, particularly where religious affiliations may be shifting rather than stable. Moreover, it has been suggested that belief could be laid aside in favor of the notion of commitment, wherein the latter term encompasses presence, embodiment, shared social location, and the like. This paper argues that while the discourse oscillates between belief on the one hand and commitment on the other, the intermediating term between these might be community. There are social and spiritual divisions, which the available discourse does not immediately allow us to contend with. In the words of one Dalit Catholic, the church must be with its people, the Bishop-Shepherd must ‘smell’ his sheep. This paper will explore how it is precisely the absence of community that Dalit Catholics experience when they find that Christian equality becomes physical separation and Christian fraternity remains outside the social domain and will suggest the implications this has for the anthropology of Christianity.
Towards Contextualized Islamic Leadership: Paraguiding and the Universities and Muslim Seminaries Project (UMSEP)
Authors: Scott-Baumann Alison , Ebbiary Alyaa , Ad Duha Mohammad Shams , Dhorat Safiyya , Begum Shahanaz , Pandor Hasan , Stolyar Julia
Published: December 2019

The Universities and Muslim Seminaries Project (UMSEP) addresses three key issues in the narrative of Muslim communal identity and religious leadership in Britain today: firstly, the need for the accreditation of Darul Ulooms (Muslim seminaries) and external validation of their programmes; secondly, understanding the career trajectories of Darul Uloom graduates, and exploring good practice; thirdly, understanding emerging leadership models in the British Muslim community. This project is a community-led, positive response to a large Arts and Humanities Research Council (AHRC)-funded research project (Re/presenting Islam on campus) conducted between 2015–2018, which identified discrimination against Muslim staff and students and the politicization of their identity due to counter terror securitisation measures. The community project summarized here in interim form proposes powerful and informed antidotes to discrimination: pathways to mutual recognition in higher education. We used interviews, workshops, and surveys and triangulated our findings to draw our draft conclusions. Firstly, we found enough interest in universities and Darul Ulooms to proceed with accreditation for an Islamic course with the same standing as a degree. Secondly, we identified barriers to career pathways for Muslims. Thirdly, we developed new models of Muslim community leadership, most notably Muslim chaplaincy with spiritual components: a career path with specific significance for Muslim women.
Heterogeneity Detection Method for Transmission Multispectral Imaging Based on Contour and Spectral Features
Authors: Wang Yanjun , Li Gang , Yan Wenjuan , He Guoquan , Lin Ling
Published: December 2019

Transmission multispectral imaging (TMI) has potential value for medical applications, such as early screening for breast cancer. However, because biological tissue has strong scattering and absorption characteristics, the heterogeneity detection capability of TMI is poor. Many techniques, such as frame accumulation and shape function signal modulation/demodulation techniques, can improve detection accuracy. In this work, we develop a heterogeneity detection method by combining the contour features and spectral features of TMI. Firstly, the acquisition experiment of the phantom multispectral images was designed. Secondly, the signal-to-noise ratio (SNR) and grayscale level were improved by combining frame accumulation with shape function signal modulation and demodulation techniques. Then, an image exponential downsampling pyramid and Laplace operator were used to roughly extract and fuse the contours of all heterogeneities in images produced by a variety of wavelengths. Finally, we used the hypothesis of invariant parameters to do heterogeneity classification. Experimental results show that these invariant parameters can effectively distinguish heterogeneities with various thicknesses. Moreover, this method may provide a reference for heterogeneity detection in TMI.
Methodological Nationalism in Global Studies and Beyond
Authors: Koos Agnes Katalin , Keulman Kenneth
Published: December 2019

Global studies, or the study of globalization, is a diverse field of research, with different disciplinary focuses and with some national versions. Russian Alexander Chumakov constructed it as a philosophical discipline, while in U.S. academia it is considered an empirical inquiry at the intersection of area studies, international studies, and international relations. This paper focuses on American global studies, pointing out the heavy epistemological burden it inherited from the field of knowledge dominated by international relations, which enshrines both methodological and political nationalism. International relations makes claims to be the sole theory originator in this field, but it may be criticized for several methodological and ethical issues (such as unwarranted simplifications that purge empirical contents to the point of unfalsifiability, antiquated epistemic ideals, Western and hegemonic biases, besides methodological nationalism), thus alternate theorizations are highly desirable.
Constraints on the String T-Duality Propagator from the Hydrogen Atom
Authors: Wondrak Michael F. , Bleicher Marcus
Published: December 2019

We investigated the implications of string theory in the high-precision regime of quantum mechanics. In particular, we examined a quantum field theoretical propagator which was derived from string theory when compactified at the T-duality self-dual radius and which is closely related to the path integral duality. Our focus was on the hydrogen ground state energy and the 1 S 1 / 2 − 2 S 1 / 2 transition frequency, as they are the most precisely explored properties of the hydrogen atom. The T-duality propagator alters the photon field dynamics leading to a modified Coulomb potential. Thus, our study is complementary to investigations where the electron evolution is modified, as in studies of a minimal length in the context of the generalized uncertainty principle. The first manifestation of the T-duality propagator arises at fourth order in the fine-structure constant, including a logarithmic term. For the first time, constraints on the underlying parameter, the zero-point length, are presented. They reach down to 3.9 × 10 − 19 m and are in full agreement with previous studies on black holes.
Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil
Authors: Aragão Carine Fortes , Pinheiro Valéria Cristina Soares , Nunes Neto Joaquim Pinto , Silva Eliana Vieira Pinto da , Pereira Glennda Juscely Galvão , Nascimento Bruna Laís Sena do , Castro Karoline da Silva , Maia Ariadne Mendonça , Catete Clistenes Pamplona , Martins Lívia Carício , Tadei Wanderli Pedro , Silva Sandro Patroca da , Cruz Ana Cecília Ribeiro
Published: December 2019

Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.