bluuebunny/crossref_metadata_2025 huggingface dataset:
HbA1c Variability as an Independent Correlate of Nephropathy, but Not Retinopathy, in Patients With Type 2 Diabetes
Authors: Penno Giuseppe , Solini Anna , Bonora Enzo , Fondelli Cecilia , Orsi Emanuela , Zerbini Gianpaolo , Morano Susanna , Cavalot Franco , Lamacchia Olga , Laviola Luigi , Nicolucci Antonio , Pugliese Giuseppe ,   for the Renal Insufficiency And Cardiovascular Events (RIACE) Study Group
Published: March 2013

OBJECTIVE

To examine the association of hemoglobin (Hb) A1c variability with microvascular complications in the large cohort of subjects with type 2 diabetes from the Renal Insufficiency And Cardiovascular Events (RIACE) Italian Multicenter Study.

RESEARCH DESIGN AND METHODS

Serial (3–5) HbA1c values collected in a 2-year period before enrollment were available from 8,260 subjects from 9 centers (of 15,773 patients from 19 centers). HbA1c variability was measured as the intraindividual SD of 4.52 ± 0.76 values. Diabetic retinopathy (DR) was assessed by dilated funduscopy. Chronic kidney disease (CKD) was defined based on albuminuria, as measured by immunonephelometry or immunoturbidimetry, and estimated glomerular filtration rate (eGFR) was calculated from serum creatinine.

RESULTS

Median and interquartile range of average HbA1c (HbA1c-MEAN) and HbA1c-SD were 7.57% (6.86–8.38) and 0.46% (0.29–0.74), respectively. The highest prevalence of microalbuminuria, macroalbuminuria, reduced eGFR, albuminuric CKD phenotypes, and advanced DR was observed when both HbA1c parameters were above the median and the lowest when both were below the median. Logistic regression analyses showed that HbA1c-SD adds to HbA1c-MEAN as an independent correlate of microalbuminuria and stages 1–2 CKD and is an independent predictor of macroalbuminuria, reduced eGFR, and stages 3–5 albuminuric CKD, whereas HbA1c-MEAN is not. The opposite was found for DR, whereas neither HbA1c-MEAN nor HbA1c-SD affected nonalbuminuric CKD.

CONCLUSIONS

In patients with type 2 diabetes, HbA1c variability affects (albuminuric) CKD more than average HbA1c, whereas only the latter parameter affects DR, thus suggesting a variable effect of these measures on microvascular complications.
Randomized Assessment of Ticagrelor Versus Prasugrel Antiplatelet Effects in Patients With Diabetes
Authors: Alexopoulos Dimitrios , Xanthopoulou Ioanna , Mavronasiou Eleni , Stavrou Katerina , Siapika Argyro , Tsoni Evropi , Davlouros Periklis
Published: March 2013

OBJECTIVE

It has been postulated that prasugrel might be the preferred treatment option in diabetes mellitus (DM) patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI). We aimed to compare the pharmacodynamic action of ticagrelor versus prasugrel.

RESEARCH DESIGN AND METHODS

In a prospective, single-center, single-blind, crossover study, 30 consecutive ACS patients with DM who had been pretreated with clopidogrel were randomized to either 90 mg ticagrelor twice daily or 10 mg prasugrel once daily with a 15-day treatment period. Platelet reactivity (PR) was assessed with the VerifyNow P2Y12 function assay, measured in P2Y12 reaction units (PRU).

RESULTS

PR was significantly lower after ticagrelor (45.2 PRU [95% CI 27.4–63.1]) compared with prasugrel (80.8 PRU [63.0–98.7]), with a least squares mean difference of –35.6 PRU (−55.2 to −15.9, P = 0.001). High PR rate was 0% for ticagrelor and 3.3% for prasugrel (P = 1.0).

CONCLUSIONS

In DM patients with ACS who had been pretreated with clopidogrel and who undergo PCI, ticagrelor achieves a significantly higher platelet inhibition than prasugrel. Both antiplatelet agents effectively treat high PR. The relevance of these findings to the clinical efficacy and safety of ticagrelor and prasugrel in DM patients needs further elucidation.
Histopathologic Delineation of the Transition Zone in Short-Segment Hirschsprung Disease
Authors: Kapur Raj P. , Kennedy Amy J.
Published: March 2013

Failure to completely resect the transition zone (TZ) between aganglionic and neuroanatomically normal bowel (“TZ pull-through”) is considered one reason for postoperative obstructive symptoms in Hirschsprung disease (HD). Despite years of study, the proximal boundary of the TZ remains nebulous, complicated by discordant, often subjective, histopathologic definitions. In order to objectively delineate the TZ, transverse sections at 1 cm intervals from the rectums of 9 non-HD autopsy subjects and resections from 15 infants with short-segment HD were immunostained with Hu (ganglion cell bodies) and glucose transporter 1 (Glutl) (perineurium of extrinsic nerves), and 6 putative features of TZ were examined: (1) aganglionosis of ≥1/8th circumference; (2) myenteric or submucosal hypoganglionosis; (3) hypertrophic submucosal nerves; (4) Glut1+ submucosal innervation; (5) submucosal hyperganglionosis; and (6) “ectopic” ganglia in lamina propria, muscularis propria, or serosa. In non-HD controls, Glut1+ submucosal innervation, hypertrophic nerves, partial circumferential aganglionosis, and hypoganglionosis were absent or restricted to the distal 2 cm. In contrast, all 6 neuropathologic features of TZ were identified proximal to the aganglionic segment in the majority of HD resections, but the length of the TZ ranged from 0 to 12 cm, depending on which neuropathologic feature was considered. Excluding submucosal hyperganglionosis and ectopic ganglia, the TZ was generally <5 cm. Many features of TZ cannot be excluded intraoperatively with a biopsy or a full-circumference frozen section. However, partial circumferential aganglionosis, severe myenteric hypoganglionosis, and hypertrophic submucosal nerves can, and probably should, be assessed in full-circumference frozen sections of the proximal resection margin, to reduce the likelihood of TZ pull-through.
Cribriform Neuroepithelial Tumor Arising in the Lateral Ventricle
Authors: Arnold Michael A. , Stallings-Archer Kandi , Marlin Evan , Grondin Ronald , Olshefski Randal , Biegel Jaclyn A. , Pierson Christopher R.
Published: March 2013

Cribriform neuroepithelial tumor (CRINET) is a recently recognized central nervous system neoplasm that arises in the ventricles of young children and is characterized by primitive, non-rhabdoid SMARCB1-deficient cells with prominent cribriform architecture. We report a 14-month-old male who presented with signs of increased intracranial pressure. Neuroimaging revealed a large solid and cystic mass in the lateral ventricle. Tumor cells were small, primitive appearing, and arranged in cribriform and trabecular patterns with interspersed solid cellular areas. Rhabdoid cells were absent. Immunohistochemical staining showed no SMARCB1 (INI11, BAF47, hSNF5) expression and strong epithelial membrane antigen (EMA) immunoreactivity along luminal surfaces. Electron microscopy showed epithelial characteristics, including abundant basal lamina. Genetic analysis of the tumor revealed deletion of 1 SMARCB1 allele, while the other contained an intronic point mutation predicted to disrupt splicing. This case further illustrates the distinct histopathologic and molecular genetic features of CRINET and identifies distinctive ultrastructural features.
Phenotypic and Genotypic Characterisation of Pasteurella Multocida Strains Isolated from Pigs in Poland
Authors: Stępniwska Katarzyna , Markowska-Daniel Iwona
Published: March 2013

A total of 319 Pasteurella multocida (Pm) strains isolated from pigs in Poland were examined. Phenotypic characterisation included: biochemical tests (to determine species, subspecies, and biovar), capsular typing, and antimicrobial susceptibility. Genotypic characterisation included detection of the toxA gene by PCR. All tested Pm strains were classified as Pm subsp. multocida: 87.2% biovar 3, 10.7%-2 and 0.9%-12. One strain was classified as biovar 1. Three strains of Pm did not suit any of the biovars. Using capsular typing methods, 77% of Pm strains isolated from nasal swabs belonged to type D and 33% to type A. Among Pm strains isolated from internal organs, 59.5% belonged to type A and 40.5% to type D. All the isolates showed a high susceptibility to β-lactams: ampicillin and amoxicilin with clavulonic acid (97.8%), penicillin (86.7%), doxicilline (100%), oxytetracycline (97.8%), and tetracycline (93.2%). It was found that all strains were susceptible to norfloxacin, 97.8% to enrofloxacin, and 95.6% to SxT. 24.4% and 15.6% of the strains were resistant to linco-spectin and tiamulin, respectively. The presence of toxA gene was confirmed by PCR in 20.8% of the strains isolated from nasal swabs and 29.1% of isolates from internal organs.
Elastase, Myeloperoxidase, and Alkaline Phosphatase Release and Free Radical Generation in Neutrophils Isolated from Blood of Sows at Different Stages of Oestrous Cycle
Authors: Wessely-Szponder Joanna , Bobowiec Ryszard
Published: March 2013

The aim of the study was to assess the influence of the oestrous cycle phase on neutrophil secretory activity and to extrapolate it to susceptibility to uterine infections. The obtained results indicate that the highest enzyme release seen in the late follicular phase (elastase release was 42.18 ±3.11% of maximal release, myeloperoxidase was 45.0 ±5.12%, and alkaline phosphatase was 44.75 ±9.0%) was related to the level of 17β-oestradiol in plasma. Similarly, a free radical generation was also the most enhanced during this phase. Significantly lower values were obtained from sows during the luteal phase in regard to both enzyme release (36.62 ±3.58% for elastase, 27.87 ±8.7% for myeloperoxidase, and 22.12 ±2.4% for alkaline phosphatase), and that of free radicals (2.28 1.6 μM/106 cells for nitric oxide and 2.47 0.6 nM/106 cells for superoxide).
Variability of Selected Clinical and Haematological Indices in Young Stallions During 100-Day Performance Test
Authors: Janicki Bogdan , Kochowicz Anna , Buzała Mateusz , Krumrych Wiesław
Published: March 2013

The study was conducted on 20 clinically healthy stallions, aged 3-4 years. The evaluation of the degree of adaptation of stallions to exercise loads in the course of a 100-d training programme was conducted three times: (I) - on the 1st d of training, (II) - on the 46th d of training, and (III) - on the 97th d of training. Rectal temperature (T) and number of heart and respiratory rates were measured. Blood was sampled before exercise (1), after exercise (2), and 30 min after rest (3). The following indices were determined in the blood: concentration of haemoglobin, haematocrit level, number of red blood cells, white blood cells, lymphocytes, neutrophils, eosinophils, basophils, monocytes, and platelets. Post-exertion changes in the evaluated indicators showed moderate intensity to the applied loads with respect to functional potential of tested horses. The effect of the standard 100-d training was to stimulate the body's adaptation to functional tasks, which was evidenced, among others, by lower variability of post-exertion T and the number of red blood cells. The highest T increase was found at the beginning of the programme (about 2.1%), whereas during the subsequent studies (II and III) it was significantly (P<0.05) lower (0.98% and 0.84%, respectively). The variability in red blood cell count in the course of training showed a downward trend (16.4%, 13.7%, and 11.0%, respectively). The results confirm the relatively low exercise loads and good health of young stallions during the 100-d performance test.
Influence of Beta-Endorphin on Oxygen Activity of Neutrophils and Total Antioxidant Status in Rats after Chronic Administration of Methimazole
Authors: Gołyński Marcin , Lutnicki Krzysztof
Published: March 2013

The aim of the study was to evaluate the effect of β-endorphin on oxygen activity of polimorphonuclear neutrophils and to investigate the total antioxidant status of blood serum in rats subjected to chronic treatment with methimazole. The study was carried out on 15 male Wistar rats weighing 270-290 g, divided into two groups. The animals from experimental group were administered a 0.05% drinking water solution of methimazole. The oxygen activity of peripheral blood neutrophils was examined using a modified RBA test. The extra-receptor effect of β-endorphin on the respiratory burst of neutrophils was evaluated. The incubation of neutrophils in the presence of phorbol myristate acetate (PMA) in the control group caused a nearly threefold increase in the oxygen activity of the cells compared to the control. Slightly higher values were obtained after addition of β-endorphin at the concentration of 10-6. Lower concentrations of this opioid (10-8 and 10-10) also stimulated oxygen activity but at a lower level than PMA. Naloxone did not affect the oxygen activity only when using β-endorphin in the concentration of 10-6. Naloxone and β-endorphin in the concentrations of 10-8 and 10-10 evoked a linear decrease in the oxygen activity of neutrophils, below the values obtained for PMA. The obtained results suggest that methimazole significantly modulates the generation of reactive oxygen species under the influence of β-endorphin, which stimulates the respiratory burst via the receptor pathway and inhibits it via the extra-cellular pathway. Methimazole also stimulates the anti-free radical defence.
Adriamycin activity's durational governance of different cell death types and zonality in rat liver acinus. Immunohistochemical studies
Authors: Pedrycz Agnieszka , Boratyński Zbigniew , Siermontowski Piotr , Mendocha Jacek , Orłowski Marcin , Van Damme-Ostapowicz Katarzyna
Published: March 2013

The aim of this study was to develop and examine a model of apoptosis and necrosis of hepatocytes induced by a damaging factor - adriamycin, correlating time after its administration with cell death type, and to investigate the localisation within the liver acinus of hepatocytes dying in these two ways. The results obtained in the present and previous studies were compared in order to make a map of cell death localisation in the liver acinus, showing the effect of time in action and dose of adriamycin. The experiment was performed on 32 female Wistar rats, divided into four groups: I and II - experimental, and III and IV - control. Adriamycin (3 mg/kg b.w.) was administered intraperitoneally to rats in groups I and II, and the rats were decapitated after four (group I) and eight (group II) weeks. Animals in control groups III and IV were given 0.5 mL of 0.9% NaCl solution, and decapitated after four and eight weeks respectively. Sections of the liver were examined with a three-stage immunohistochemical method. This method allowed to examine hepatocytes qualitatively and quantitatively for the presence of proteins involved in three types of apoptosis: induced by the mitochondrial pathway (caspase 3, 9), the intrinsic pathway related to endoplasmic reticulum stress (caspase 3, 12), and the extrinsic pathway (caspase 3, 8). One of the inflammatory markers, caspase 1, was also examined. The zonal localisation of all three types of apoptosis was assessed in the liver tissue. More oxidated hepatocytes indicated only signs of the internal mitochondrial pathway, whereas less oxidated hepatocytes induced the internal reticular pathway and the external apoptotic pathway. The period between adriamycin administration and hepatic cell investigation was a main factor of the process. A longer period post insult resulted in a more pronounced effect of the activation of apoptosis. Sections explored eight weeks after treatment with different doses of the drug (3 and 5 mg/kg in the previous study) showed a similar intensity of apoptosis.
Ultrastructure of Renal Tubular Epithelial Cells Of Rat’s Kidneys after Administration of L-Arginine
Authors: Pedrycz Agnieszka , Boratyński Zbigniew , Siermontowski Piotr , Kaczerska Dorota
Published: March 2013

Sixteen white Wistar female rats were divided into two equal groups. Experimental group received per os 40 mg/kg b.w. of L-arginine, every other day for 2 weeks and were decapitated after 3 weeks of the experiment. Control rats received in the same manner 2 ml of distilled water and were decapitated after 3 weeks of the experiment. The renal lesions observed under electron microscope were of focal character and concerned only the experimental group. The tubules with necrotic cells were observed among normal tubules or single normal epithelial cells of the tubular wall. The boundaries between epithelial cells of the tubule wall were blurred. The mitochondria indicated abnormal structure. Numerous lysosomes and peroxysomes with dark, homogenous content were observed. The rough endoplasmic reticulum had widened channels and was focally completely destroyed. The nucleus of damaged cells was most commonly located in one of the cell poles; its shape was changed and visibly smaller than the nuclei of normal cells. Condensation and peripherally located chromatin were noticed. The lesions observed were characteristic for apoptotic cells.
A Fuzzy Approach for Bidding Strategy Selection
Authors: Ilieva Galina
Published: March 2013

The goal of this work is to explore the applicability of fuzzy logic in multiagent systems for choosing the best bidding strategy in electronic auction. To find the multi-criterion ordering, agents use a fuzzy algorithm ARAKRI2 with direct aggregation operators MaxMin and MinAvg. The key difference between this new approach and known from the literature solution FTNA is in the lack of weighted coefficients. Despite the difference both algorithms give results that are similar. Therefore, the proposed approach can successfully solve the task for multi criteria selection of bidding strategy.
Eco-hydrological impacts of Eucalyptus in the semi humid Ethiopian Highlands: the Lake Tana Plain
Authors: Chanie Tilashwork , Collick Amy S. , Adgo Enyew , Lehmann C. Johannes , Steenhuis Tammo S.
Published: March 2013

Eucalyptus is the tree of choice for wood production by farmers in Ethiopia. Although there are many claims about its harmful effect on ecology and water availability, little actual research exists. The main objective of this study was, therefore, to study the extent of harm of Eucalyptus on the ecosystem. This study was conducted at the Koga Watershed near Lake Tana in Ethiopia. Twenty-five farmers were interviewed and a field experiment with three replications was carried out to quantify the effect of Eucalyptus on various soil physical and chemical properties and maize crop measurements and to compare bulk density, soil moisture contents, maize crop counts and shading effects in fields bordered by Eucalyptus and Croton macrostachyus. Our results show that Eucalyptus decreased both soil nutrients and maize yields within 20 m of the trees. Although moisture content was not affected during the monsoon, it decreased faster within 30 m of the Eucalyptus trees than elsewhere. Soils become water repellent, too. Local farmers’ perception agreed with our experimental findings and indicated that Eucalyptus trees are exhausting the once productive land. They also reported that Eucalyptus dries up springs. Despite this, the growers insist on planting Eucalyptus because of its cash income.
Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus
Authors: Ptak Agata , Tahchy Anna , Skrzypek Edyta , Wójtowicz Tomasz , Laurain-Mattar Dominique
Published: March 2013

In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 µM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 µM of dicamba to 100% for picloram (for both 25 and 50 µM). 2,4-D (50 µM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 µM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 µM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 µM), picloram (25 µM), and dicamba (50 µM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 µM).
Effect of nitrate on lipid production by T. suecica, M. contortum, and C. minutissima
Authors: Sánchez-García Didier , Resendiz-Isidro Anayelli , Villegas-Garrido Thelma , Flores-Ortiz César , Chávez-Gómez Benjamín , Cristiani-Urbina Eliseo
Published: March 2013

Microalgae are an alternative and sustainable source of lipids that can be used as a feedstock for biodiesel production. Nitrate is a good nitrogen source for many microalgae and affects biomass and lipid yields of microalgae. In this study, the effect of nitrate on cell growth and lipid production and composition in Monoraphidium contortum, Tetraselmis suecica, and Chlorella minutissima was investigated. Nitrate affected the production of biomass and the production and composition of lipids of the three microalgae tested. Increasing the nitrate concentration in the culture medium resulted in increased biomass production and higher biomass productivity. Furthermore, increasing the nitrate concentration resulted in a reduction in lipid content and productivity in M. contortum; however, the opposite effect was observed in T. suecica and C. minutissima cultures. C. minutissima and M. contortum lipids contain high levels of oleic acid, with values ranging from 26 to 45.7% and 36.4 to 40.1%, respectively. The data suggest that because of its high lipid productivity (13.79 mg L−1 d−1) and high oleic acid productivity (3.78 mg L−1 d−1), Chlorella minutissima is a potential candidate for the production of high quality biodiesel.
Long term and seasonal ground deformation monitoring of Larissa Plain (Central Greece) by persistent scattering interferometry
Authors: Vassilopoulou Spyridoula , Sakkas Vasileios , Wegmuller Urs , Capes Ren
Published: March 2013

The land subsidence which occurs at the Larissa Basin (Thessaly Plain, Central Greece) is due to various causes including aquifer system compaction. Deformation maps of high spatial resolution deduced by the Persistent Scattering Interferometry (PSI) technique (using radar scenes from ERS and ENVISAT satellites) for the period 1992–2006 were produced to study the spatial and temporal ground deformation.

A developed GIS database (including geological, tectonic, morphological, hydrological, meteorological and watertable variation from wells in the area) offered the possibility of studying in detail the intense subsidence. The PSI based average deformation image clearly shows that subsidence generally takes place inside the Larissa Plain ranging from 5–250 mm. The largest amplitude rates (−25 mm/yr) are observed around the urban area of Larissa City (especially at Gianouli and Nikea villages), while the Larissa City center appears to be relatively stable with a tendency to subside. The rest of the plain regions seem to subside at moderate rates (about 5–10 mm/yr). The surrounding mountainous area is stable, or has slightly been uplifted with respect to the NE located reference point. It was found that there is a correlation between the seasonal water-table variation (deduced from wells data), the seasonal water demand for irrigation associated with specific types of cultivation (cotton fields), the monthly rainfall, and the observed subsidence rate in the rural regions of the Thessaly Plain.
Response of Rat Erythrocyte Oxidative Stress Markers to Repetitive Hyperbaric Oxygen Exposures Up to 40 Daily Sessions / Odgovor Markera Oksidativnog Stresa U Eritrocitima Pacova Na Višestruko Izlaganje Hiperbaričnom Kiseoniku Do 40 Seansi Dnevno
Authors: Simsek Kemal , Yildirim Ali Osman , Demirbas Seref , Oztosun Muzaffer , Ozler Mehmet , Ozkan Esin , Oter Sukru , Korkmaz Ahmet , Ay Hakan , Yildiz Senol
Published: March 2013

Background: Studies with single-session hyperbaric oxygen exposures have shown that HBO-induced oxidative stress is proportional to exposure pressure and duration. Since the efficacy of hyperbaric oxygen mainly depends on repetitive exposures, this study aimed to investigate the oxidative effect of hyperbaric oxygen administered for 5 to 40 sessions.

Methods: Sixty rats were divided into one control and 6 study groups. Study groups were exposed to 5, 10, 15, 20, 30, and 40 daily consecutive 2.8 atm/90 min hyperbaric oxygen sessions. Animals were sacrificed 24 h after the last hyperbaric oxygen administration. Malondialdehyde and carbonylated protein levels as well as superoxide dismutase activities were determined in isolated rat erythrocytes.

Results: Carbonylated protein levels increased significantly after just 5 hyperbaric oxygen exposures; reached a peak level with 10 exposures; were still significantly higher than controls after 15 sessions; and decreased to normal limits after 20 exposures. Malondialdehyde levels were found to be significantly increased in the 10 to 30, but not in the 5 and 40-session groups. Superoxide dismutase activity showed elevated levels only in the 5 and 10 times hyperbarical oxygen-exposed groups.

Conclusions: The suppressed oxidative stress level after 40 exposures suggests an effective endogenous antioxidant defense in repetitive HBO administrations.
Influence of Investment Projects on Dividend Policy of a Company in Conditions of Fuzzy Information
Authors: Jabłoński Bartłomiej
Published: March 2013

This article describes the problem of using fuzzy logic in the forecasts of variants of the results of implemented investment projects. It also shows the possibility of using calculations with the application of fuzzy triangular numbers L-R in the variants of results: an optimistic result, a pessimistic result and the most probable result (the central result). Moreover, the article draws attention to the possibility of using fuzzy numbers to estimate fuzzy risk of a forecasted financial result of a company. In addition, the article presents how a dividend may be shaped in consideration of multiplicity of variants if dividend policy takes into account dependence on company results as well as implemented investment projects.
Cardiovascular risk in rheumatoid arthritis – evaluation and treatment
Authors: Matei Luminiţa , Parepa Irinel , Șuța Cristina , Șuța Maria
Published: March 2013

Introduction: Rheumatoid arthritis patients have an increased risk of cardiovascular morbidity and mortality. Consecrated cardiovascular risk management strategies are underused in this patients. The purpose of this paper is to evaluate, in rheumatoid arthritis hospitalized patients, the level of cardiovascular risk and the degree of implementation of currently recommended cardiovascular risk reduction treatments.

Materials and methods: 130 active rheumatoid arthritis patients, consecutively admitted in the Rheumatology Clinic of our hospital, were evaluated based on clinical exam, routine biochemistry, X-ray, electrocardiogram and cardiac ultrasound. We noted the characteristics of the rheumatic disease, the cardiovascular risk factors and organic heart disease. The risk for cardiac death at 10 years was estimated using the SCORE table; and adequate use of cardiovascular risk reduction therapies (antiaggregants, statin, antihypertensive medication) was checked.

Results: Organic heart disease was identified in 28.4% of the patients. The most frequent encountered risk factors were dyslipidemia (62.3%), hypertension (54.6%) and abdominal obesity (53%). Globally, 66,1% of the patients were classified as having high cardiovascular risk. Hypertension and dyslipidemia were treated in 81.6%, respective 40.7% of the cases and controlled to recommended therapeutic goals in 70.4%, respective 22.2%. Among the patients with a symptomatic atherosclerotic disease, less than one half (48.6%) were treated with antiaggregants.

Conclusion: Organic heart disease was frequent among rheumatoid arthritis patients; the most prevalent cardiovascular risk factors were hypertension, dyslipidemia and abdominal obesity. The treatment and control rates for hypertension were acceptable, but statins and antiaggregants were grossly underused.
Reading, Writing, and Friendship: Adult Implications of Effective Literacy Instruction for Students with Intellectual Disability
Authors: Forts Ann M. , Luckasson Ruth
Published: March 2013

Reading and literacy are important not only for instrumental reasons such as knowing exit signs and recognizing initial consonants but also have tremendous human functioning implications in areas such as initiating and sustaining friendships, communicating care and affection, and enhancing work, leisure, and play. Many people with intellectual disability are denied opportunities to learn literacy, which in turn denies richness in life opportunities. This article describes one friendship in which reading and literacy play an important part. We describe some of our experiences learning to read and engaging in lifelong literacy and how literacy affects our friendship and our ability to express affection for others and brings us enjoyment.
Supported Employment for Young Adults with Autism Spectrum Disorder: Preliminary Data
Authors: Wehman Paul , Lau Stephanie , Molinelli Alissa , Brooke Valerie , Thompson Katie , Moore Chandler , West Michael
Published: March 2013

The purpose of this study was to examine the effects of supported employment in securing and maintaining competitive employment for people with autism spectrum disorder, a group that has typically been found to be underemployed or unemployed. This prospective study followed and collected data on 33 individuals with autism spectrum disorder as they progressed through a supported employment model, working one-on-one with an employment specialist. Of the 33 individuals included in the study, 27 successfully obtained competitive employment, with a total of 29 positions secured. The successful results were achieved through the use of a supported employment model and skilled employment specialists who were able to provide a high level of social supports and compensatory training strategies for skill acquisition. Specifically, employment specialists supported individuals through four steps of an individualized supported employment model: (a) the development of a jobseeker profile and assessment, (b) guiding the job development and career search, (c) conducting job site training, and (d) designing long-term supports to promote job retention. The multitude and variance of specific methods and strategies used in each case to execute these key steps of the supported employment model accurately reflect an emphasis on a highly individualized approach. Although the outcomes of this preliminary study were positive in terms of employment outcomes, further research remains to be conducted.
The Doubting Dance: Contributions to a History of Parent/Professional Interactions in Early 20th Century America
Authors: Ferguson Philip M.
Published: March 2013

The relationship of professionals with families of children and adults with intellectual disabilities is a key component in the planning and delivery of effective and enduring support services. Yet, the history of such relationships is not well understood. After briefly describing the context for these relationships in the early 20th century, a thematic analysis is presented of correspondence between parents and professionals at one institution for people with intellectual disabilities during the early 1900s. The study concludes with a brief discussion of what we can learn from this troubled history to improve family/professional relationships.
Increasing Communication in Children with Concurrent Vision and Hearing Loss
Authors: Brady Nancy C. , Bashinski Susan M.
Published: March 2013

Nine children with complex communication needs and concurrent vision and hearing losses participated in an intervention program aimed at increasing intentional prelinguistic communication. The intervention constituted a pilot, descriptive study of an adapted version of prelinguistic milieu teaching, hence referred to as A-PMT. In A-PMT, natural gestures and vocalizations were targeted in child-focused, one-on-one activities conducted by a member of the project staff. Adaptations included using more physical prompts than in other forms of PMT and using means other than directed eye gaze to determine directionality of gestures. All nine participants increased their rates of initiated, intentional communication substantially during the course of intervention; in addition, each participant acquired new forms of natural gestures. Results were limited primarily to requests (as opposed to other communication functions). Discussion centers on how to promote more generalized communication developments in future implementations of the program.
The Effects of Computer-Based Instruction on the Transition Planning Process Knowledge of Parents of Students with Disabilities
Authors: Rowe Dawn A. , Test David W.
Published: March 2013

Enhancing parents' ability to make informed choices is important to successful transition planning for youth with disabilities. This study used a multiple probe design across content areas replicated across participants to examine the effects of a computer-based instructional program on acquisition of parents' knowledge of the transition planning process (i.e., postsecondary goals, transition services, and postsecondary transition service providers). Results suggested a functional relation between computer-based instruction and parent knowledge of postsecondary goals, transition services, and postsecondary transition service providers. Suggestions for future research and implications for practice are discussed.
La Déconstruction de la scène de bataille dans les Aventures de Télémaque (1699) de François Fénelon
Authors: Worvill Romira
Published: March 2013

L’auteur des Aventures de Télémaque a semé son épopée de « tableaux », c’est-à-dire de passages de description qui sont inspirés d’une connaissance approfondie des beaux-arts et qui supposent des connaissances similaires chez le lecteur. On examine ici les techniques picturales employées par François Fénelon dans les scènes de bataille qui déterminent l’attitude du jeune protagoniste envers la guerre et les guerriers. Il s’avère que la représentation des divers « spectacles » de la guerre auxquels réagit Télémaque rappelle les trois tendances stylistiques incarnées dans des tableaux faisant hommage aux exploits militaires de Louis xiv: ceux de Charles le Brun, d’Adam Frans Van der Meulen et de Joseph Parrocel. La comparaison des descriptions de Fénelon avec les toiles de ces peintres suggère que l’auteur des Aventures de Télémaque cherchait à combattre non seulement l’idéologie de la gloire guerrière qui dominait le milieu du jeune Duc de Bourgogne, mais aussi l’influence séduisante des images militaristes dont il était entouré.
Book Reviews
Authors: Jung Theo , Fernández Sebastián Javier
Published: March 2013

Matthias Kroß and Rüdiger Zill, eds., Metapherngeschichten. Perspektiven
einer Theorie der Unbegrifflichkeit [Histories of Metaphor: Perspectives of a
Theory of Nonconceptuality] (Berlin: Parerga Verlag, 2011), 259 pp.
Decision-making in farming households in eastern Poland
Authors: Krzyworzeka Amanda
Published: March 2013

The agricultural situation in Poland has been changing significantly during the last decades. In 1989, the predictability of the communist centrally planned economy was replaced by the unexpectedness and "invisible hand" of the free market economy. The socialist welfare state has been replaced by new modes of support, introduced by European Union (EU). On the basis of fieldwork conducted between 2005 and 2008 in farming communities in eastern Poland, I focus on decision making among small-scale farmers. This article addresses decision-making processes and their sociocultural context, including the reasons for and circumstances behind decisions, and also elements of decision-making processes that tend to hinder the introduction of EU agricultural policy. In the course of adapting to new and changing realities, farmers creatively use customary ways of thinking and acting in the various decisions they have to make while running the farm. Changes of the very mechanisms of decision-making processes seem to be rather slow, however.
The Greek economic crisis as trope
Authors: Knight Daniel M.
Published: March 2013

The Greek economic crisis resonates across Europe as synonymous with corruption, poor government, austerity, financial bailouts, civil unrest, and social turmoil. The search for accountability on the local level is entangled with competing rhetorics of persuasion, fear, and complex historical consciousness. Internationally, the Greek crisis is employed as a trope to call for collective mobilization and political change. Drawing on ethnographic research conducted in Trikala, central Greece, this article outlines how accountability for the Greek economic crisis is understood in local and international arenas. Trikala can be considered a microcosm for the study of the pan-European economic turmoil as the “Greek crisis“ is heralded as a warning on national stages throughout the continent.
The Effects of Inhibiting Hedgehog Signaling Pathways by Using Specific Antagonist Cyclopamine on the Chondrogenic Differentiation of Mesenchymal Stem Cells
Authors: Wu Xing , Cai Zheng-Dong , Lou Lei-Ming , Chen Zheng-Rong
Published: March 2013

This study aimed to investigate the effects of cyclopamine, a specific inhibitor of Hedgehog signaling pathways, on the chondrogenic differentiation of mesenchymal stem cells (MSCs). During culture, the experimental groups were treated with cyclopamine and their cell proliferation status was assessed using the MTT test. The extra-bone cellular matrix (ECM) and Collagen II (Col II) was detected by toluidine blue staining and immunohistochemistry of cells. The concentrations of Col II and aggrecan in the culture solution and cytosol were detected using ELISA on the 7th, 14th, and 21st days of cyclopamine induction. Gene and protein expression of Col II and aggrecan were analyzed on the 14th day of cyclopamine induction using real-time PCR and western blot analyses. No significant differences in proliferation of mesenchymal stem cells were found between the control group and the group treated with cyclopamine. Compared to the blank control group, the ECM level was low and the protein and mRNA concentrations of Collagen II (Col II) and aggrecan in the culture solution and cytosol, respectively, were significantly reduced in the experimental group. The Smo acted as a key point in the regulations of Hedgehog signaling pathway on the chondrogenic differentiation of rabbit MSCs.
Inhibitory Effect of Baicalin and Baicalein on Ovarian Cancer Cells
Authors: Chen Jianchu , Li Zhaoliang , Chen Allen , Ye Xingqian , Luo Haitao , Rankin Gary , Chen Yi
Published: March 2013

Ovarian cancer is one of the primary causes of death for women all through the Western world. Baicalin and baicalein are naturally occurring flavonoids that are found in the roots and leaves of some Chinese medicinal plants and are thought to have antioxidant activity and possible anti-angiogenic, anti-cancer, anxiolytic, anti-inflammatory and neuroprotective activities. Two kinds of ovarian cancer (OVCAR-3 and CP-70) cell lines and a normal ovarian cell line (IOSE-364) were selected to be investigated in the inhibitory effect of baicalin and baicalein on cancer cells. Largely, baicalin and baicalein inhibited ovarian cancer cell viability in both ovarian cancer cell lines with LD50 values in the range of 45–55 µM for baicalin and 25–40 µM for baicalein. On the other hand, both compounds had fewer inhibitory effects on normal ovarian cells viability with LD50 values of 177 µM for baicalin and 68 µM for baicalein. Baicalin decreased expression of VEGF (20 µM), cMyc (80 µM), and NFkB (20 µM); baicalein decreased expression of VEGF (10 µM), HIF-1α (20 µM), cMyc (20 µM), and NFkB (40 µM). Therefore baicalein is more effective in inhibiting cancer cell viability and expression of VEGF, HIF-1α, cMyc, and NFκB in both ovarian cancer cell lines. It seems that baicalein inhibited cancer cell viability through the inhibition of cancer promoting genes expression including VEGF, HIF-1α, cMyc, and NFκB. Overall, this study showed that baicalein and baicalin significantly inhibited the viability of ovarian cancer cells, while generally exerting less of an effect on normal cells. They have potential for chemoprevention and treatment of ovarian cancers.
Marine-Derived Angiogenesis Inhibitors for Cancer Therapy
Authors: Wang Ying-Qing , Miao Ze-Hong
Published: March 2013

Angiogenesis inhibitors have been successfully used for cancer therapy in the clinic. Many marine-derived natural products and their analogues have been reported to show antiangiogenic activities. Compared with the drugs in the clinic, these agents display interesting characteristics, including diverse sources, unique chemical structures, special modes of action, and distinct activity and toxicity profiles. This review will first provide an overview of the current marine-derived angiogenesis inhibitors based on their primary targets and/or mechanisms of action. Then, the marine-derived antiangiogenic protein kinase inhibitors will be focused on. And finally, the clinical trials of the marine-derived antiangiogenic agents will be discussed, with special emphasis on their application potentials, problems and possible coping strategies in their future development as anticancer drugs.
Synthesis of Peptide Radiopharmaceuticals for the Therapy and Diagnosis of Tumor Diseases
Authors: Jamous Mazen , Haberkorn Uwe , Mier Walter
Published: March 2013

Despite the advances in molecular biology and biochemistry, the prognosis of patients suffering from tumor diseases remains poor. The limited therapeutic success can be explained by the insufficient performance of the common chemotherapeutic drugs that lack the ability to specifically target tumor tissues. Recently peptide radiopharmaceuticals have been developed that enable the concurrent imaging and therapy of tumors expressing a specific target. Here, with a special emphasis on the synthesis of the building blocks required for the complexation of metallic radioisotopes, the requirements to the design and synthesis of radiolabeled peptides for clinical applications are described.
Article Synthesis and Trypanocidal Activity of Novel 2,4,5-Triaryl-N-Hydroxylimidazole Derivatives
Authors: Da Silva Ramon , Loback Vanessa , Salomão Kelly , De Castro Solange , Wardell James , Wardell Solange , Costa Thadeu , Penido Carmen , De Henriques Maria , Carvalho Samir , Da Silva Edson , Fraga Carlos
Published: March 2013

Herein, we report the design, synthesis and trypanocidal activity of some novel trisubstituted imidazole derivatives. These heterocyclic derivatives were structurally planned by exploring the concept of molecular hybridisation between two arylhydrazones derived from megazol, which has potent trypanocidal activity. The trypanocidal activity of these triarylimidazole derivatives was evaluated against infective trypomastigote forms of T. cruzi and the derivative 2'-(4-bromophenyl)-1-methyl-5'-phenyl-1H,3'H-2,4'-biimidazol-3'-ol showed moderate biological activity (IC50 = 23.9 µM) when compared to benznidazole, a standard trypanocidal drug. These compounds did not present cytotoxic effects at concentrations near the trypanocidal IC50, being considered a good starting point for the development of new anti-Chagas drug candidates.
The ABC of Vitamin D: A Qualitative Study of the Knowledge and Attitudes Regarding Vitamin D Deficiency amongst Selected Population Groups
Authors: Bonevski Billie , Bryant Jamie , Lambert Sylvie , Brozek Irena , Rock Vanessa
Published: March 2013

Objective: In Australia, vitamin D supply in food is limited, and sun exposure is the main source of vitamin D. However skin cancer risk is high, and the need to gain some sun exposure for adequate vitamin D is challenging public health messages to use protection in the sun. The complex vitamin D public health message may be confusing the public and, in particular, those at highest risk for vitamin D deficiency. This study explored vitamin D and sun exposure attitudes, knowledge and practices of some groups considered at risk of vitamin D deficiency and those delivering healthy sun exposure messages to children. Method: 52 adults participated in six focus groups. Results: Results corroborated with previous research showing low levels of vitamin D knowledge. Individual and environmental barriers to receiving adequate sun exposure were also identified. Conclusions and Implications: The message advocating balanced sun exposure to produce adequate vitamin D needs to be made clearer and be more effectively communicated. Findings provide insights to aid development of appropriate public health messages for safe sun exposure and vitamin D, especially for vulnerable groups.
Humanized Mouse Models of Epstein-Barr Virus Infection and Associated Diseases
Authors: Fujiwara Shigeyoshi , Matsuda Go , Imadome Ken-Ichi
Published: March 2013

Epstein-Barr virus (EBV) is a ubiquitous herpesvirus infecting more than 90% of the adult population of the world. EBV is associated with a variety of diseases including infectious mononucleosis, lymphoproliferative diseases, malignancies such as Burkitt lymphoma and nasopharyngeal carcinoma, and autoimmune diseases including rheumatoid arthritis (RA). EBV in nature infects only humans, but in an experimental setting, a limited species of new-world monkeys can be infected with the virus. Small animal models, suitable for evaluation of novel therapeutics and vaccines, have not been available. Humanized mice, defined here as mice harboring functioning human immune system components, are easily infected with EBV that targets cells of the hematoimmune system. Furthermore, humanized mice can mount both cellular and humoral immune responses to EBV. Thus, many aspects of human EBV infection, including associated diseases (e.g., lymphoproliferative disease, hemophagocytic lymphohistiocytosis and erosive arthritis resembling RA), latent infection, and T-cell-mediated and humoral immune responses have been successfully reproduced in humanized mice. Here we summarize recent achievements in the field of humanized mouse models of EBV infection and show how they have been utilized to analyze EBV pathogenesis and normal and aberrant human immune responses to the virus.