bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitochondrial Respiration in Response to Iron Deficiency Anemia: Comparison of Peripheral Blood Mononuclear Cells and Liver
Authors: Fischer Christine , Valente de Souza Lara , Komlódi Timea , Garcia-Souza Luiz F. , Volani Chiara , Tymoszuk Piotr , Demetz Egon , Seifert Markus , Auer Kristina , Hilbe Richard , Brigo Natascha , Petzer Verena , Asshoff Malte , Gnaiger Erich , Weiss Günter
Published: March 2022

Iron is an essential component for metabolic processes, including oxygen transport within hemoglobin, tricarboxylic acid (TCA) cycle activity, and mitochondrial energy transformation. Iron deficiency can thus lead to metabolic dysfunction and eventually result in iron deficiency anemia (IDA), which affects approximately 1.5 billion people worldwide. Using a rat model of IDA induced by phlebotomy, we studied the effects of IDA on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the liver. Furthermore, we evaluated whether the mitochondrial function evaluated by high-resolution respirometry in PBMCs reflects corresponding alterations in the liver. Surprisingly, mitochondrial respiratory capacity was increased in PBMCs from rats with IDA compared to the controls. In contrast, mitochondrial respiration remained unaffected in livers from IDA rats. Of note, citrate synthase activity indicated an increased mitochondrial density in PBMCs, whereas it remained unchanged in the liver, partly explaining the different responses of mitochondrial respiration in PBMCs and the liver. Taken together, these results indicate that mitochondrial function determined in PBMCs cannot serve as a valid surrogate for respiration in the liver. Metabolic adaptions to iron deficiency resulted in different metabolic reprogramming in the blood cells and liver tissue.
Dihydrolipoamide Acetyltransferase AceF Influences the Type III Secretion System and Resistance to Oxidative Stresses through RsmY/Z in Pseudomonas aeruginosa
Authors: Li Haozhou , Xia Yushan , Tian Zhenyang , Jin Yongxin , Bai Fang , Cheng Zhihui , Swietnicki Wieslaw , Wu Weihui , Pan Xiaolei
Published: March 2022

Carbon metabolism plays an important role in bacterial physiology and pathogenesis. The type III secretion system (T3SS) of Pseudomonas aeruginosa is a virulence factor that contributes to acute infections. It has been demonstrated that bacterial metabolism affects the T3SS. Meanwhile, expression of T3SS genes is negatively regulated by the small RNAs RsmY and RsmZ. In this study, we studied the relationship between the dihydrolipoamide acetyltransferase gene aceF and the T3SS. Our results reveal an upregulation of RsmY and RsmZ in the aceF mutant, which represses the expression of the T3SS genes. Meanwhile, the aceF mutant is more tolerant to hydrogen peroxide. We demonstrate that the expression levels of the catalase KatB and the alkyl hydroperoxide reductase AhpB are increased in the aceF mutant. The simultaneous deletion of rsmY and rsmZ in the aceF mutant restored the expression levels of katB and ahpB, as well as bacterial susceptibility to hydrogen peroxide. Thus, we identify a novel role of AceF in the virulence and oxidative response of P. aeruginosa.
Ultrastable Conjugated Microporous Polymers Containing Benzobisthiadiazole and Pyrene Building Blocks for Energy Storage Applications
Authors: Mohamed Mohamed Gamal , Mansoure Tharwat Hassan , Samy Maha Mohamed , Takashi Yasuno , Mohammed Ahmed A. K. , Ahamad Tansir , Alshehri Saad M. , Kim Jeonghun , Matsagar Babasaheb M. , Wu Kevin C.-W. , Kuo Shiao-Wei
Published: March 2022

In recent years, conjugated microporous polymers (CMPs) have become important precursors for environmental and energy applications, compared with inorganic electrode materials, due to their ease of preparation, facile charge storage process, π-conjugated structures, relatively high thermal and chemical stability, abundance in nature, and high surface areas. Therefore, in this study, we designed and prepared new benzobisthiadiazole (BBT)-linked CMPs (BBT–CMPs) using a simple Sonogashira couplings reaction by reaction of 4,8-dibromobenzo(1,2-c;4,5-c′)bis(1,2,5)thiadiazole (BBT–Br2) with ethynyl derivatives of triphenylamine (TPA-T), pyrene (Py-T), and tetraphenylethene (TPE-T), respectively, to afford TPA–BBT–CMP, Py–BBT–CMP, and TPE–BBT–CMP. The chemical structure and properties of BBT–CMPs such as surface areas, pore size, surface morphologies, and thermal stability using different measurements were discussed in detail. Among the studied BBT–CMPs, we revealed that TPE–BBT–CMP displayed high degradation temperature, up to 340 °C, with high char yield and regular, aggregated sphere based on thermogravimetric analysis (TGA) and scanning electron microscopy (SEM), respectively. Furthermore, the Py–BBT–CMP as organic electrode showed an outstanding specific capacitance of 228 F g−1 and superior capacitance stability of 93.2% (over 2000 cycles). Based on theoretical results, an important role of BBT–CMPs, due to their electronic structure, was revealed to be enhancing the charge storage. Furthermore, all three CMP polymers featured a high conjugation system, leading to improved electron conduction and small bandgaps.
Reduction in Allergenicity and Induction of Oral Tolerance of Glycated Tropomyosin from Crab
Authors: Han Xin-Yu , Bai Tian-Liang , Yang Huang , Lin Yi-Chen , Ji Nai-Ru , Wang Yan-Bo , Fu Ling-Lin , Cao Min-Jie , Liu Jing-Wen , Liu Guang-Ming
Published: March 2022

Tropomyosin (TM) is an important crustacean (Scylla paramamosain) allergen. This study aimed to assess Maillard-reacted TM (TM-G) induction of allergenic responses with cell and mouse models. We analyzed the difference of sensitization and the ability to induce immune tolerance between TM and TM-G by in vitro and in vivo models, then we compared the relationship between glycation sites of TM-G and epitopes of TM. In the in vitro assay, we discovered that the sensitization of TM-G was lower than TM, and the ability to stimulate mast cell degranulation decreased from 55.07 ± 4.23% to 27.86 ± 3.21%. In the serum of sensitized Balb/c mice, the level of specific IgE produced by TM-G sensitized mice was significantly lower than TM, and the levels of interleukins 4 and interleukins 13 produced by Th2 cells in spleen lymphocytes decreased by 82.35 ± 5.88% and 83.64 ± 9.09%, respectively. In the oral tolerance model, the ratio of Th2/Th1 decreased from 4.05 ± 0.38 to 1.69 ± 0.19. Maillard reaction masked the B cell epitopes of TM and retained some T cell epitopes. Potentially, Maillard reaction products (MRPs) can be used as tolerance inducers for allergen-specific immunotherapy.
Double-Focusing Gradient-Index Lens with Elastic Bragg Mirror for Highly Efficient Energy Harvesting
Authors: Park Jeonghoon , Lee Geon , Lee Dongwoo , Kim Miso , Rho Junsuk
Published: March 2022

The applicability of piezoelectric energy harvesting is increasingly investigated in the field of renewable energy. In improving harvester efficiency, manipulating elastic waves through a geometric configuration as well as upgrading harvester elements is important. Periodic structures, such as phononic crystals and metamaterials, are extensively employed to control elastic waves and enhance harvesting performance, particularly in terms of wave localization and focusing. In this study, we propose a double-focusing flexural energy harvesting platform consisting of a gradient-index lens and elastic Bragg mirror. Based on the design process, the frequency and time response of the harvesting platform are analyzed. The results indicate that the output voltage and power calculated at 1800 Ω are 7.9 and 62 times higher than those observed in the bare plate, respectively. Even when compared to the existing gradient-index system, they are 1.5 and 2.3 times higher, respectively. These findings can facilitate the usage of periodic structures as geometric stimuli to significantly enhance harvesting performance.
Nonlinear Optical Rectification in an Inversion-Symmetry-Broken Molecule near a Metallic Nanoparticle
Authors: Domenikou Natalia , Thanopulos Ioannis , Yannopapas Vassilios , Paspalakis Emmanuel
Published: March 2022

We study the nonlinear optical rectification of an inversion-symmetry-broken quantum system interacting with an optical field near a metallic nanoparticle, exemplified in a polar zinc–phthalocyanine molecule in proximity to a gold nanosphere. The corresponding nonlinear optical rectification coefficient under external strong field excitation is derived using the steady-state solution of the density matrix equations. We use ab initio electronic structure calculations for determining the necessary spectroscopic data of the molecule under study, as well as classical electromagnetic calculations for obtaining the influence of the metallic nanoparticle to the molecular spontaneous decay rates and to the external electric field applied to the molecule. The influence of the metallic nanoparticle to the optical rectification coefficient of the molecule is investigated by varying several parameters of the system, such as the intensity and polarization of the incident field, as well as the distance of the molecule from the nanoparticle, which indirectly affects the molecular pure dephasing rate. We find that the nonlinear optical rectification coefficient can be greatly enhanced for particular incident-field configurations and at optimal distances between the molecule and the metallic nanoparticle.
Deformation Mechanism of Depositing Amorphous Cu-Ta Alloy Film via Nanoindentation Test
Authors: Li Weibing , Wang Xiao , Feng Xiaobin , Du Yao , Zhang Xu , Xie Yong , Chen Xiaoming , Lu Yang , Wang Weidong
Published: March 2022

As a representative of immiscible alloy systems, the Cu-Ta system was the research topic because of its potential application in industry, military and defense fields. In this study, an amorphous Cu-Ta alloy film was manufactured through magnetron sputter deposition, which was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Mechanical properties of Cu-Ta film were detected by the nanoindentation method, which show that the elastic modulus of Cu3.5Ta96.5 is 156.7 GPa, and the hardness is 14.4 GPa. The nanoindentation process was also simulated by molecular dynamic simulation to indicate the deformation mechanism during the load-unload stage. The simulation results show that the structure <0,2,8,4> and <0,2,8,5> Voronoi cells decreased by 0.1% at 50 Ps and then remained at this value during the nanoindentation process. In addition, the number of dislocations vary rapidly with the depth between indenter and surface. Based on the experimental and simulation results, the Voronoi structural changes and dislocation motions are the key reasons for the crystallization of amorphous alloys when loads are applied.
Highly Selective Removal of Cationic Dyes from Wastewater by MgO Nanorods
Authors: Ghoniem Monira Galal , Ali Fatima Adam Mohamed , Abdulkhair Babiker Yagoub , Elamin Mohamed Rahmt Allah , Alqahtani Arwa Mofareh , Rahali Seyfeddine , Ben Aissa Mohamed Ali
Published: March 2022

The organic synthetic dyes employed in industries are carcinogenic and harmful. Dyes must be removed from wastewater to limit or eliminate their presence before dumping into the natural environment. The current study aims to investigate the use of MgO nanoparticles to eliminate basic fuchsine (BF), as a model cationic dye pollutant, from wastewater. The MgO nanorods were synthesized through a coprecipitation method. The obtained nanocomposite was characterized using various techniques such as X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Brunauer–Emmett–Teller (BET), and FTIR spectroscopy. It was found that the variation of dye concentration and pH influenced the removal of BF by MgO. The adsorption capacity of 493.90 mg/g is achieved under optimum operating conditions (pH = 11, contact time = 236 min, and initial BF concentration = 200 ppm). Pseudo-second-order adsorption kinetics and Freundlich isotherm models best fitted BF sorption onto MgO nanorods. The BF sorption mechanism is associated with the electrostatic attractions and hydrogen bond between the O–H group of MgO and the NH2 groups of BF, as indicated by the pH, isotherms, and FTIR studies. The reusability study indicates that MgO was effectively used to eliminate BF in at least four continuous cycles. The investigation of MgO with different dyes suggests the high adsorption selectivity of BF, crystal violet (CV), and malachite green (MG) dyes compared with methyl orange (MO) dye. Overall, MgO nanorods can act as a potential and promising adsorbent for the efficient and rapid removal of cationic dyes (CV, MG, and BF) from wastewater.
Resonant Tunneling Diodes: Mid-Infrared Sensing at Room Temperature
Authors: Rothmayr Florian , Guarin Castro Edgar David , Hartmann Fabian , Knebl Georg , Schade Anne , Höfling Sven , Koeth Johannes , Pfenning Andreas , Worschech Lukas , Lopez-Richard Victor
Published: March 2022

Resonant tunneling diode photodetectors appear to be promising architectures with a simple design for mid-infrared sensing operations at room temperature. We fabricated resonant tunneling devices with GaInAsSb absorbers that allow operation in the 2–4 μm range with significant electrical responsivity of 0.97 A/W at 2004 nm to optical readout. This paper characterizes the photosensor response contrasting different operational regimes and offering a comprehensive theoretical analysis of the main physical ingredients that rule the sensor functionalities and affect its performance. We demonstrate how the drift, accumulation, and escape efficiencies of photogenerated carriers influence the electrostatic modulation of the sensor’s electrical response and how they allow controlling the device’s sensing abilities.
Highly Sensitive and Stable Humidity Sensor Based on the Bi-Layered PVA/Graphene Flower Composite Film
Authors: Rahman Sheik Abdur , Khan Shenawar Ali , Rehman Muhammad Muqeet , Kim Woo-Young
Published: March 2022

Two-dimensional (2D) materials and their composites have gained significant importance as the functional layer of various environmental sensors and nanoelectronics owing to their unique properties. This work reports for the first time a highly sensitive, fast, and stable humidity sensor based on the bi-layered active sensing area composed of graphene flower (GF) and poly (vinyl alcohol) PVA thin films for multifunctional applications. The GF/PVA humidity sensor exhibited stable impedance response over 15 days, for a relative humidity (RH) range of (40–90% RH) under ambient operating conditions. The proposed bi-layered humidity sensor also exhibited an ultra-high capacitive sensitivity response of the 29 nF/%RH at 10 kHz and fast transient response of 2 s and 3.5 s, respectively. Furthermore, the reported sensor also showed a good response towards multi-functional applications such as non-contact skin humidity and mouth breathing detection.
Application of Mini-LEDs with Microlens Arrays and Quantum Dot Film as Extra-Thin, Large-Area, and High-Luminance Backlight
Authors: Chen Yen Lung , Ye Zhi Ting , Lai Wei , Chiu Chang Che , Lin Kuo Wei , Han Pin
Published: March 2022

The demand for extra-thin, large-area, and high-luminance flat-panel displays continues to grow, especially for portable displays such as gaming laptops and automotive displays. In this paper, we propose a design that includes a light guide layer with a microstructure above the mini-light-emitting diode light board. The light control microstructure of concave parabel-surface microlens arrays on a light-emitting surface increases the likelihood of total internal reflection occurring and improved the uniformity merit function. We used a 17 in prototype with quantum-dot and optical films to conduct our experiments, which revealed that the thickness of the module was only 1.98 mm. When the input power was 28.34 watts, the uniformity, average luminance, and CIE 1931 color space NTSC of the prototype reached 85%, 17,574 cd/m2, and 105.37%, respectively. This module provided a flat light source that was extra thin and had high luminance and uniformity.
Experimental Study on the Quenching Behavior of a Copper Cube in the Cellulose Nanofiber Solution
Authors: Choi Hundong , Jeong Subin , Lee Kwon-Yeong
Published: March 2022

This study investigates the quenching behavior and heat transfer characteristics of a copper cube immersed in cellulose nanofiber (CNF) solutions. The heat transfer performance of CNF solutions during boiling has been examined in several studies, but the quenching behavior of CNF solutions, which is an important heat transfer method, has not been evaluated. In this study, four copper cubes with the same dimensions and four different quenchants (deionized water and 0.01%, 0.1% and 0.5% CNF solutions) were prepared. A copper cube heated to greater than 600 °C was submerged three times in the quenchant. This was repeated with different copper cubes in each of the quenchants. The time at which the heated copper cube convectively transferred heat to the surroundings was recorded. The cooling time was shortest when the cube was immersed in a 0.5% CNF solution for the first time. The average cooling time for quenching in the 0.5% CNF solution was 30.3% shorter than that in DI water. In this study, film boiling during quenching was thoroughly observed and analyzed to explain the cause of enhancement in heat transfer efficiency.
The Biological Traumatization of Crops Due to the Enzyme Stage of Enzyme-Mycotic Seed Depletion
Authors: Temirbekova Sulukhan K. , Kulikov Ivan M. , Afanasyeva Yuliya V. , Ashirbekov Mukhtar Z. , Beloshapkina Olga O. , Kalashnikova Elena A. , Sardarova Irina , Begeulov Marat Sh. , Kucher Dmitry E. , Ionova Natalia E. , Rebouh Nazih Y.
Published: March 2022

In the light of Vavilov’s Law, grain traumatization in the standing crop of wheat and other crops due to the enzyme stage of enzyme-mycotic seed depletion (EMSD) was confirmed, the parameters of open and hidden harmfulness were detected, and a scale of plant resistance to such traumatization was developed. The current study demonstrates that pathogen contamination in grains occurs before harvesting and its degree is determined by favorable humidity and temperature conditions and by the open and hidden grain traumatization due to the enzyme stage of EMSD, i.e., the grain’s hydrolytic enzymes providing a growth substrate for a fungal spread that is later substituted by pathogen enzymes leading to grain spoiling and self-warming. The most common technique to preserve grain quality is to support a moisture level that prevents further spreading of the fungi. The grains that are contaminated with very low temperature and humidity levels facilitate the germinability and high quality of the grain. The new ways to withstand EMSD should, first of all, include a selection of activities. Using biological, biochemical and physical (X-ray) methods, genetic sources of resistance towards EMSD were found in the VIR world collection that is recommended for further selection. These sources have become a basis for the varieties, such as Moskovskaya 39, Ilot (winter wheat), Gremme and Gremme 2U (hulless spelt), Alcoran (winter spelt) and Kanysh (spring wheat).
Pathogenicity and Its Implications in Taxonomy: The Brucella and Ochrobactrum Case
Authors: Moreno Edgardo , Blasco José María , Letesson Jean Jacques , Gorvel Jean Pierre , Moriyón Ignacio
Published: March 2022

The intracellular pathogens of the genus Brucella are phylogenetically close to Ochrobactrum, a diverse group of free-living bacteria with a few species occasionally infecting medically compromised patients. A group of taxonomists recently included all Ochrobactrum organisms in the genus Brucella based on global genome analyses and alleged equivalences with genera such as Mycobacterium. Here, we demonstrate that such equivalencies are incorrect because they overlook the complexities of pathogenicity. By summarizing Brucella and Ochrobactrum divergences in lifestyle, structure, physiology, population, closed versus open pangenomes, genomic traits, and pathogenicity, we show that when they are adequately understood, they are highly relevant in taxonomy and not unidimensional quantitative characters. Thus, the Ochrobactrum and Brucella differences are not limited to their assignments to different “risk-groups”, a biologically (and hence, taxonomically) oversimplified description that, moreover, does not support ignoring the nomen periculosum rule, as proposed. Since the epidemiology, prophylaxis, diagnosis, and treatment are thoroughly unrelated, merging free-living Ochrobactrum organisms with highly pathogenic Brucella organisms brings evident risks for veterinarians, medical doctors, and public health authorities who confront brucellosis, a significant zoonosis worldwide. Therefore, from taxonomical and practical standpoints, the Brucella and Ochrobactrum genera must be maintained apart. Consequently, we urge researchers, culture collections, and databases to keep their canonical nomenclature.
Single-Pixel Moving Object Classification with Differential Measuring in Transform Domain and Deep Learning
Authors: Yao Manhong , Zheng Shujun , Hu Yuhang , Zhang Zibang , Peng Junzheng , Zhong Jingang
Published: March 2022

Due to limited data transmission bandwidth and data storage space, it is challenging to perform fast-moving objects classification based on high-speed photography for a long duration. Here we propose a single-pixel classification method with deep learning for fast-moving objects. The scene image is modulated by orthogonal transform basis patterns, and the modulated light signal is detected by a single-pixel detector. Thanks to the property that the natural images are sparse in the orthogonal transform domain, we used a small number of basis patterns of discrete-sine-transform to obtain feature information for classification. The proposed neural network is designed to use single-pixel measurements as network input and trained by simulation single-pixel measurements based on the physics of the measuring scheme. Differential measuring can reduce the difference between simulation data and experiment data interfered by slowly varying noise. In order to improve the reliability of the classification results for fast-moving objects, we employed a measurement data rolling utilization approach for repeated classification. Long-duration classification of fast-moving handwritten digits that pass through the field of view successively is experimentally demonstrated, showing that the proposed method is superior to human vision in fast-moving digit classification. Our method enables a new way for fast-moving object classification and is expected to be widely implemented.
Enhance Trial: Effects of NAD3® on Hallmarks of Aging and Clinical Endpoints of Health in Middle Aged Adults: A Subset Analysis Focused on Blood Cell NAD+ Concentrations and Lipid Metabolism
Authors: Roberts Michael D. , Osburn Shelby C. , Godwin Joshua S. , Ruple Bradley A. , La Monica Michael B. , Raub Betsy , Sandrock Jennifer E. , Ziegenfuss Tim N. , Lopez Hector L.
Published: March 2022

Limited pre-clinical and clinical data suggest theacrine or theacrine-based supplements modulate biological processes associated with lipid metabolism and aging. Herein, we sought to examine if 12 weeks of daily supplementation with a theacrine-based supplement (termed NAD3®; 312 mg of combined Wasabia japonica freeze-dried rhizome standardized for isothicyantes, theacrine, and copper (I)niacin chelate) altered serum lipids as well as select nicotinamide adenine dinucleotide (NAD+)-associated metabolites in peripheral blood mononuclear cells (PBMCs). Twenty-eight participants (12 males, 16 females) were randomly assigned to receive either NAD3 (n = 13; age: 52 ± 7 years old, body mass index: 29.0 ± 5.0 kg/m2) or a cellulose placebo (n = 15; age: 51 ± 5 years old, body mass index: 28.3 ± 3.9 kg/m2). Blood samples were obtained in mornings following overnight fasts prior to supplementation (Pre) and following the 12-week intervention (Post). PBMCs were freshly isolated and prepared for targeted NAD+ metabolomics, and serum as well as whole blood was assayed for blood lipids and other safety markers through a commercial laboratory. Significant interactions (p < 0.05) were observed for total cholesterol, LDL cholesterol, and LDL: HDL ratio and post hoc analyses indicated these biomarkers significantly decreased with NAD3 supplementation (Pre-to-Post percent decreases were 11.1, 15.2, and −18.9%, respectively). A significant interaction was also observed for PBMC NAD+: NADH values, where levels trended downward from Pre to Post in the CTL group (p = 0.081) and values at Post were greater in NAD3 versus CTL (p = 0.023). No interactions were observed for systolic/diastolic blood pressure, body mass, or blood markers indicative of clinical safety. Although participant numbers were limited, these first-in-human data demonstrate a theacrine-based NAD3 supplement can favorably alter biomarkers of lipid metabolism and cellular NAD+ status. However, the latter data are limited to targeted NAD+ metabolites, and the effects of supplementation on other cellular metabolites or mechanisms related to the observed outcomes need to be further explored.
The Reactions of Photosynthetic Capacity and Plant Metabolites of Sedum hybridum L. in Response to Mild and Moderate Abiotic Stresses
Authors: Terletskaya Nina V. , Seitimova Gulnaz A. , Kudrina Nataliya O. , Meduntseva Nataliya D. , Ashimuly Kazhybek
Published: March 2022

In this article, for the first time, an experimental study of the effect of mild and moderate osmotic stress, NaCl content and the effect of low positive temperature on photosynthetic activity and composition of metabolites of immature plants Sedum hybridum L. is reported. In this representative of the genus Sedum adapted to arid conditions and having the properties of a succulent, a change in photosynthetic activity and an increase in the level of protective metabolites in the shoots were revealed when exposed to mild and moderate stress factors. The results of this study can be used in work on the adaptation of succulent plants to arid conditions, environmental monitoring and work on the directed induction of valuable secondary metabolites in succulents to obtain new herbal medicines.
Effects Induced by the Agricultural Application of Probiotics on Antioxidant Potential of Strawberries
Authors: Mockevičiūtė Rima , Jurkonienė Sigita , Gavelienė Virgilija , Jankovska-Bortkevič Elžbieta , Šocik Božena , Armalytė Gabija , Budrys Rimas
Published: March 2022

With the recent rapid development of the functional food sector, agriculture is looking for alternatives to improve the quality of food grown by limiting chemical fertilizers. This study evaluated the effects of two commercial plant probiotics, ProbioHumus and NaturGel, on the growth and quality of strawberry fruits. Strawberry plants were sprayed with microbial probiotics twice a year: after harvesting at the beginning of dormancy and at the stage of leaf development. Spray applications of ProbioHumus, NaturGel, and NaturGel + ProbioHumus in the organic farm fields significantly increased the fresh fruit weight up to 42%, 35%, and 37%, respectively, compared to the non-treated control. An increase in the weight of fresh strawberry fruits may be associated with an increase in dry matter accumulation. The probiotics had a positive effect on the total content of phenols, anthocyanins, and especially ascorbic acid in strawberry fruits. The increase in ascorbic acid in strawberry fruits was up to 97% compared to the non-treated control. The fruits from plants inoculated with probiotics showed significantly higher antioxidant activity. In summary, ProbioHumus and NaturGel are effective tools for improving the quality of strawberries and can be exploited in sustainable agriculture as a tool for adding value to functional food.
Niche Variation in Endemic Lilium pomponium on a Wide Altitudinal Gradient in the Maritime Alps
Authors: Fontaine Ninon , Gauthier Perrine , Casazza Gabriele , Thompson John D.
Published: March 2022

The relationship between altitudinal and ecological gradients has long been a dominant theme in plant ecology; moreover, how species respond to climate change has renewed this interest. Mediterranean mountains are often hotspots of endemism, and some endemic species have local distributions that span different climatic belts; hence, local variations in topography and fine-scaled niche conditions may play crucial roles in their persistence along such gradients. Studies of the fine-scaled niche are, however, very rare; most studies involve broad-scale variations in climatic parameters. The Turban lily, Lilium pomponium L. is endemic to the Maritime and Ligurian Alps, where it occurs across a wide altitudinal gradient. Previous work has shown no link between climatic marginality and geographic range limits on morphological traits and genetic variability; however, possible variations of local topographic and ecological parameters have not yet been examined. The objective of this paper is to characterise local ecological niche conditions of L. pomponium populations in the different bioclimatic zones it occupies along the altitudinal gradient. The species occurs in four main types of microecological niches. One of these niche types, with a high mineral cover, is the most abundant—type 2: it was detected in 39% of sampled quadrats and occurs across the whole bioclimatic gradient. Other niche types are more limited to subsections of the gradient: type 3 (in 19% of sampled quadrats) is restricted to high-altitude sites (>1070 m.a.s.l.) and is characterised by high vegetation and litter cover; type 4 (26%) corresponds to more forested habitats on substrates with low water retention capacities, in more inland zones close to the centre of L. pomponium distribution and across a range of altitudes; and type 1 (16% of quadrat) only occurs in the Mediterranean part of the gradient, close to distribution limits in pockets of soil among large blocks of rocks, mainly found at mid-altitudes. Despite heterogeneity in the spatial locations of niche types, there is no correspondence between ecological gradients and the distribution limits of this species. Knowledge of the fine-scaled ecological conditions that determine niche types is thus essential for conservation management of the habitats of this species and for the exploration of its possible response to ongoing climate change.
Production and Secretion of Isowighteone in Hairy Root Cultures of Pigeon Pea (Cajanus cajan) Co-Treated with Multiple Elicitors
Authors: Gajurel Gaurav , Nopo-Olazabal Luis , Hendrix Emily , Medina-Bolivar Fabricio
Published: March 2022

Isowighteone (3’-isoprenyl genistein) is a prenylated flavonoid derivative that exhibits antibacterial, pro-apoptotic and anti-inflammatory properties. To establish a bioproduction system for this bioactive compound, hairy root cultures of pigeon pea (Cajanus cajan (L.) Millsp.) were developed via Agrobacterium rhizogenes-mediated transformation. The cultures were co-treated with methyl jasmonate, cyclodextrin, hydrogen peroxide, and magnesium chloride to enhance the production of isowighteone. The amount of isowighteone that accumulated in the culture medium upon elicitation varied with the period of elicitation. Isowighteone was purified from extracts of the culture medium by semi-preparative HPLC, and its identity was confirmed by tandem mass spectrometry. After 144 h of elicitation in 12-day-old hairy root culture, the total yield of isowighteone was 8058.618 ± 445.78 μg/g DW, of which approximately 96% was found in the culture medium. The yield of isowighteone in the elicited hairy root culture was approximately 277-fold higher than in the non-elicited root culture. The difference between the phenotypes of the elicited and non-elicited pigeon pea hairy roots was studied using scanning electron microscopy. The non-elicited hairy roots had uniform surfaces whereas the elicited roots had non-uniform shapes. Pigeon pea hairy roots provide a sustainable platform for producing and studying the biosynthesis of isowighteone.
Progress in Aromatic Polyimide Films for Electronic Applications: Preparation, Structure and Properties
Authors: Wu Ziyu , He Jianjun , Yang Haixia , Yang Shiyong
Published: March 2022

Aromatic polyimides have excellent thermal stability, mechanical strength and toughness, high electric insulating properties, low dielectric constants and dissipation factors, and high radiation and wear resistance, among other properties, and can be processed into a variety of materials, including films, fibers, carbon fiber composites, engineering plastics, foams, porous membranes, coatings, etc. Aromatic polyimide materials have found widespread use in a variety of high-tech domains, including electric insulating, microelectronics and optoelectronics, aerospace and aviation industries, and so on, due to their superior combination characteristics and variable processability. In recent years, there have been many publications on aromatic polyimide materials, including several books available to readers. In this review, the representative progress in aromatic polyimide films for electronic applications, especially in our laboratory, will be described.
A Multicriteria Decision Trading System Based on Prospect Theory: A Risk Return Analysis of the TODIM Method
Authors: Puppo Bruna , Leles Michel , Mozelli Leonardo , Sbruzzi Elton
Published: March 2022

This paper proposes a trading system (TS) based on a multicriteria decision aid (MCDA) process known as TODIM, (Multicriteria Interactive Decision Making) a Portuguese acronym for interactive and multicriteria decision-making. MCDA has been employed to solve financial questions because of its ability to deal with a complex environment populated with different sorts of criteria and alternatives, such as financial markets. The aim is to propose a general and adaptive tool for supporting the trading strategies of investors and market practitioners in such an environment. The reason for selecting TODIM among the different MCDA methods is that it is based on prospect theory, which assumes that the risk profile of the investor varies according to different situations, considering the risk of loss or gain. A list of simulations using some of the most prominent Brazilian stocks is performed, and the results are compared with the Buy-and-Hold benchmark and a TS based on an ensemble method for selecting trading rules. The results show that, compared to Buy-and-Hold, a TODIM-based TS provides the same level of return with a lower level of risk exposure. The consequence is superior risk adjustment parameters. As a result, we have a model with similar results in profit, but with superior results in relation to risk-based performance, which makes the method advantageous in relation to similar ones.
Quantitative Measurement of Solids Holdup for Group A and B Particles Using Images and Its Application in Fluidized Bed Reactors
Authors: Wang Chengxiu , Li Zhihui , Wei Jianjin , Lan Xingying , Ye Mao , Gao Jinsen
Published: March 2022

Solids holdup as one of the main parameters in characterizing the performance of fluidized bed reactors is widely concerned. With its development and improvement, visualization technology has been applied in fluidization because of its little disturbance to the flow. In this study, four types of particles with different properties are tested in a narrow rectangular fluidized bed equipped with a high-speed video camera. Calibration curves of these different types of particles are achieved by correlating the grayscale of the digital images with the corresponding solids holdup. These calibration curves are further applied to obtain the average solids holdup across the sectional area and local solids holdup from the center towards the wall in both a gas-solids turbulent fluidized bed and a circulating fluidized bed to verify the results. The calibration method works well for solids holdup of different types of particles in both dense and dilute fluidization systems. This method is important to characterize the fluidization quality and reactor performance with a wide operating condition.
Gut Microbiota Research in Bipolar Disorder and Possible Implications for Precision Psychiatry: A Systematic Review
Authors: Paribello Pasquale , Pinna Federica , Carpiniello Bernardo , Manchia Mirko
Published: March 2022

Bipolar disorder (BD) is a highly disabling condition with a chronic and relapsing nature. Despite the substantial socioeconomic burden associated with BD, there are still significant research gaps in risk stratification, diagnostic accuracy, and treatment selection, all key components of precision psychiatry. One possible strategy to increase the validity of precision psychiatry approaches in BD is to increase our knowledge of disorder-associated gut microbiota perturbations. To this end, we systematically reviewed the evidence on gut microbiota alterations in relation to precision psychiatry approaches on BD. We performed a systematic review on PubMed/MEDLINE and Web of Science to identify original articles investigating the possible clinical applications of microbiota analyses for pragmatic precision psychiatry in BD. A pearl growing strategy was employed to enlarge the scope of this review. The primary search strategy yielded one paper and an additional one was identified through reference tracking. The included studies were observational, with one study of good quality. The identified results justify the efforts devolved in this area of research and underscore the need to expand these investigations through additional larger and properly designed studies.
What Role for the Sisters? Islamist Movements between Authenticity and Equality
Authors: Utvik Bjørn Olav
Published: March 2022

In mainstream Islamist discourse, there is an awkward coexistence between recognition of women as equal political actors and affirmation of a traditional Muslim view of the man as head of the family. Islamism emerged in countries where patriarchy has remained deeply engrained. Yet their stances have varied. In Morocco, female Islamists have pushed for women’s rights and a guarded opening towards cooperation with feminists. In contrast, the Muslim Brothers in Egypt have remained more conservative and female cadres have prioritised fighting any development seen as threatening the Muslim family. The Arab Spring also stirred matters regarding gender relations, as women took active part in the uprisings. In the years to come, women’s issues will likely demand ever more attention across the Arab world. How the Islamists deal with this will be pivotal in determining the future of the movements. To understand the evolving responses of the movements to this challenge, it is essential to analyse the development of mainstream Islamist discourse and practice relating to gender relations in the period leading up to the ruptures of 2011. This article will investigate the issue in the two cases of Egypt and Morocco, and seeks to understand the relationship between internal and external drivers of ideological change.
Study of Unpicked Grapes Valorization: A Natural Source of Polyphenolic Compounds and Evaluation of Their Antioxidant Capacity
Authors: Elejalde Edurne , Villarán María , Lopez-de-Armentia Iratxe , Ramón Daniel , Murillo Raquel , Alonso Rosa
Published: March 2022

Every year great amounts of high-quality wine grapes are left on the vine unpicked, and consequently lost, to control the overproduction in wine areas with limited appellation production yield. In the context of circular bioeconomy, the valorization of these grapes as a potential source of natural antioxidants is of great interest. The study carried out is focused on the polyphenolic profile characterization of different unpicked grape varieties using the ultrasound-assisted extraction technique to extract the polyphenolic fractions. Moreover, the evaluation of the antioxidant capacity by several assays was carried out: oxygen radical absorbance capacity (ORAC), stability of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing antioxidant capacity (FRAP), cupric reducing antioxidant capacity (CUPRAC) and stability of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS) assays. The results showed a strong relationship between total phenolic content and hydroxycinnamic acids (R2 = 0.9088) followed by flavan-3-ols (R2 = 0.8792) and tannins (R2 = 0.7705). The antioxidant capacity of the grapes was dependent on the total phenolic content. These results supply new information for a better understanding of the importance of giving an added value to the unpicked grapes due to their high content of polyphenols. These findings help the wine sector to consider the valorization of the unpicked grapes, classified as wastes, as an interesting source of natural antioxidants to be used as food supplements and with potential applications in the pharmaceutical industry.
Using Outsourcing Services in Manufacturing Companies
Authors: Kabus Judyta , Dziadkiewicz Michał , Miciuła Ireneusz , Mastalerz Marcin
Published: March 2022

Contemporary economic entities function in various types of cooperation systems, which are primarily aimed at creating a competitive advantage and strengthening themselves in order to meet the requirements of competitors. One solution that can make a significant difference to one’s market advantage is outsourcing. It is a response of enterprises to the constantly changing conditions of functioning in a turbulent environment and the emerging new directions and concepts in management. It should be stressed that the choice of outsourcing as a strategy means not only to outsource selected work to external entities, but first of all to retain those competencies of the company that cannot be replaced by anyone. This means that a company must retain a certain sphere of the so-called key areas of activity, which in a positive way distinguish it from the competition and allow it to build an effective market advantage. The main objective of the article is to identify the areas of operation that are the most common subject of outsourcing and the determinants that affect the choice of an outsourcing operator in manufacturing companies in Poland. The variety of aspects of the research subject matter, oscillating around the main objective, has made it necessary to formulate the following research hypotheses: Research Hypothesis H1—The basic criteria determining the selection of an outsourcing operator are: price, quality of services provided and reputation; Research Hypothesis H2—The most common subject of outsourcing is finance and security. The survey was conducted in 2020, in the pre-pandemic period, on a sample of N = 120, including owners/managers of manufacturing enterprises. A non-random sample selection was used. The questionnaires were sent to 200 companies, however, only 126 were completed, of which 6 were not completed in full and were therefore rejected. The verification of the hypothesis was carried out using the chi-square test.
Active Soft Brace for Scoliotic Spine: A Finite Element Study to Evaluate in-Brace Correction
Authors: Ali Athar , Fontanari Vigilio , Schmölz Werner , Agrawal Sunil K.
Published: March 2022

Scoliosis is a spinal disorder that is conventionally treated using rigid or soft braces. Computational methods such as finite element-based models are used to investigate the mechanics of the spine and the effect of braces. Most spinal braces are either passive, static, or rigid and do not allow mobility to the spine, resulting in muscle atrophy, skin deterioration and other spine complexities. Lack of control over the amount of force being exerted by braces on the human spine could have adverse effects. Therefore, developing an active soft brace which allows mobility to the spine while applying controlled corrective forces could be a promising solution. This study presents finite element analysis (FEA) of an active soft brace that applies corrective forces using elastic bands. The pressure exerted by the brace on the spine can be controlled by varying the tensions in the elastic bands. The elastic band tensions are controlled using low-power, lightweight, and twisted string actuators (TSAs). This study aims to demonstrate the immediate corrections induced by the soft active brace using a scoliotic spine finite element (FE) model. A FE model of the patient’s trunk was created and validated with in vitro study. The brace model was installed on the simulated trunk to evaluate in-brace correction in both sagittal and coronal planes. The brace was evaluated under various load cases by simulating the actuator action.
SIF-Based GPP Is a Useful Index for Assessing Impacts of Drought on Vegetation: An Example of a Mega-Drought in Yunnan Province, China
Authors: Li Chuanhua , Peng Lixiao , Zhou Min , Wei Yufei , Liu Lihui , Li Liangliang , Liu Yunfan , Dou Tianbao , Chen Jiahao , Wu Xiaodong
Published: March 2022

The impact of drought on terrestrial ecosystem Gross Primary Productivity (GPP) is strong and widespread; therefore, it is important to study the response of terrestrial ecosystem GPP to drought. In this paper, we compared the correlations of Sun-induced Chlorophyll fluorescence (SIF), Enhanced Vegetation Index (EVI), and Normalized Differential Vegetation Index (NDVI) with the drought index sc_PDSI, estimated GPP in Yunnan Province, China, based on SIFTOTAL data (SIF data with canopy effects eliminated), and analyzed the response characteristics of GPP to drought for one mega-drought event (2009–2011) in combination with the sc_PDSI drought index. The results show that SIF is more sensitive to drought than the NDVI and EVI; the correlation between the GPP estimated based on SIF data (GPPSIF) and the actual observed flux values (R2 = 0.83) is better than GPPGLASS and GPPLUE, and the RMSE is also lower than those two products. This drought has a serious impact on GPP, and the monthly average values of the effect of drought on GPP (GPPd) in Yunnan Province in 2009, 2010, and 2011 are −11.37 gC·m−2·month−1, −23.48 gC·m−2·month−1 and −17.92 gC·m−2·month−1, which are 8.6%, 17.48% and 13.85% of the monthly average in a normal year, respectively. The spatial variability of GPP response to drought is significant, which is mainly determined by the degree, and duration of the drought, the vegetation type, the topography, and anthropogenic factors. In conclusion, GPPSIF quickly and accurately reflects the process of this drought, and this study helps to elucidate the response of GPP to drought conditions and provides more scientific information for drought prediction and ecosystem management.
Criminality and Income Inequality in Indonesia
Authors: Sugiharti Lilik , Esquivias Miguel Angel , Shaari Mohd Shahidan , Agustin Lussi , Rohmawati Hilda
Published: March 2022

We investigate whether a nexus exists between income inequality and criminal activity in Indonesia. Additionally, we examine socioeconomic variables and potential links with criminal actions (i.e., crime rate, murder, rape, physical abuse, robbery, and fraud). We use the generalized method of moments (GMM) approach, employing data for 34 provinces in Indonesia over the period of 2010–2019. The results indicate that income inequality is associated with higher criminal activity. Overall, lower unemployment, larger investment (foreign and domestic), and higher human development (education and health) can help reduce crime in Indonesia. However, higher income can reduce physical abuse and crime rates, but theft and fraud increase with income growth. Rising unemployment increases rape, abuse, robbery, and fraud. Still, unemployment does not affect murder, suggesting that non-economic factors are dominant in explaining murder and violent crimes. Furthermore, income inequality can increase robbery and fraud, although it has no significant effects on murder, rape, and abuse. Government spending on social assistance and more efficient settlement of criminal acts can lower crime rates.