bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Optimization of Hyaluronic Acid-Poloxamer In-Situ Gel Loaded with Voriconazole Cubosomes for Enhancement of Activity against Ocular Fungal Infection
Authors: Alhakamy Nabil A. , Hosny Khaled M. , Rizg Waleed Y. , Eshmawi Bayan A. , Badr Moutaz Y. , Safhi Awaji Y. , Murshid Samar S. A.
Published: April 2022

Fungal eye infections are largely disseminated, especially in developing countries where they may leave over half a million people blind per year. The current study aims to boost the voriconazole antifungal efficiency via loading it as cubosomes (VZ-Cub) into hyaluronic acid and poloxamer-based ocular in situ gel. VZ-Cub were fabricated applying Box-Behnken design and employing phytantriol, poloxamer F127, and VZ amounts as independent variables. The produced nano vesicles were evaluated for the dependent variables of particle size (PS), entrapment efficiency (EE%), and transcorneal steady-state flux (Jss) of the VZ, and, the obtained optimal VZ-Cub was loaded into an in situ gel base to enhance its ocular residence time. The in situ gel formulation was tested for its gelation temperature, drug release behavior, transcorneal permeation effects, and antifungal activity. The optimized VZ-Cub consisted of 100 mg of phytantriol, 60 mg of poloxamer F127, and 21 mg of VZ. This formulation led to a minimum PS of 71 nm, an EE% of 66%, Jss value of 6.5 µg/(cm2·min), and stability index of 94 ± 2%. The optimized VZ-Cub-loaded in situ gel released 84% VZ after 12 h and yielded a 4.5-fold increase in drug permeation compared with the VZ aqueous dispersion. The antifungal activity, which was obtained by measuring the fungal growth inhibition zones, revealed that the VZ-Cub-loaded in situ gel formulation had a 3.89-fold increase in antifungal activity compared with the VZ dispersion. In summary, an ocular in situ gel loaded with VZ-Cub could be an effective novel nano-paradigm with enhanced transcorneal permeation and antifungal properties.
Hybrid Epoxy-Alkyl Sol–Gel Coatings Reinforced with SiO2 Nanoparticles for Corrosion Protection of Anodized AZ31B Mg Alloy
Authors: Merino Emilia , Durán Alicia , Ceré Silvia , Castro Yolanda
Published: April 2022

AZ31B Mg alloys were anodized at different potentials using an alkaline electrolyte. Then, an epoxy-alkyl silane sol reinforced with SiO2 nanoparticles was prepared by sol–gel and deposited on top of the optimized anodic layers. 1-Methyl imidazole was added to the sol to promote a partial epoxy ring aperture and improve the condensation degree of the inorganic network. The results showed the curing temperature affects the inorganic polycondensation of the organic-inorganic network; this effect was analyzed by 29Si and 13C solid-state NMR spectroscopy. Electrochemical impedance spectroscopy in 3.5 wt% NaCl solution revealed that the corrosion resistance is enhanced by the anodized process obtained for Mg alloy anodized at 100 V/2 min. However, a quick deterioration of the oxide film with immersion time was evident, showing a reduction of the protection efficiency (ηE%) of 76.5% after 16 h/immersion. The deposition of an epoxy-alkyl coating improved the ηE% up to 98.6% after 72 h/immersion. The proposed hybrid coating used for post-sealing the porous anodized Mg alloy looks like a good alternative protective barrier to control the corrosion process of Mg alloys. A suitable compromise between cross-linking network and curing temperature is necessary to obtain a good barrier coating.
Valorization of Cattle Slaughtering Industry By-Products: Modification of the Functional Properties and Structural Characteristics of Cowhide Gelatin Induced by High Hydrostatic Pressure
Authors: Gao Yanlei , Wang Liyuan , Qiu Yi , Fan Xiaona , Zhang Li , Yu Qunli
Published: April 2022

This study investigates the effects of different pressures (200, 250, 300, 350, and 400 MPa) and durations (5, 10, 15, 20, and 25 min) on the functional properties, secondary structure, and intermolecular forces of cowhide gelatin. Our results show that high hydrostatic pressure significantly affected the two, three, and four-level structures of gelatin and caused the contents of the α-helix and β-turn to decrease by 68.86% and 78.58%, respectively (p < 0.05). In particular, the gelatin at 300 MPa for 15 min had the highest gel strength, emulsification, solubility, and foaming of all the treatment conditions under study. The analysis of the surface hydrophobicity, sulfhydryl content, zeta potential, and Raman spectroscopy shows that at a pressure of 300 MPa (15 min), the hydrogen bonds and hydrophobic interactions between collagen molecules are strongly destroyed, leading to changes in the tertiary and quaternary conformation of the protein and unfolding, with the electrostatic repulsion between protein particles making the decentralized state stable. In conclusion, moderate pressure and time can significantly improve the functional and structural properties of collagen, which provides theoretical support and guidance for realizing the high-value utilization of cowhide.
A Supramolecular Hydrogel Enabled by the Synergy of Hydrophobic Interaction and Quadruple Hydrogen Bonding
Authors: Lu Liangmei , Zhou Wen , Chen Zhuzuan , Hu Yang , Yang Yu , Zhang Guangzhao , Yang Zhuohong
Published: April 2022

The increasing preference for minimally invasive surgery requires novel soft materials that are injectable, with rapid self-healing abilities, and biocompatible. Here, by utilizing the synergetic effect of hydrophobic interaction and quadruple hydrogen bonding, an injectable supramolecular hydrogel with excellent self-healing ability was synthesized. A unique ABA triblock copolymer was designed containing a central poly(ethylene oxide) block and terminal poly(methylmethacrylate) (PMMA) block, with ureido pyrimidinone (UPy) moieties randomly incorporated (termed MA-UPy-PEO-UPy-MA). The PMMA block could offer a hydrophobic microenvironment for UPy moieties in water and thus boost the corresponding quadruple hydrogen bonding interaction of Upy–Upy dimers. Owing to the synergetic effect of hydrophobicity and quadruple hydrogen bonding interaction, the obtained MA-UPy-PEO-UPy-MA hydrogel exhibited excellent self-healing properties, and injectable capability, as well as superior mechanical strength, and therefore, it holds great promise in tissue engineering applications, including in cell support and drug release.
Identification of a Novel SHANK2 Pathogenic Variant in a Patient with a Neurodevelopmental Disorder
Authors: Doddato Gabriella , Fabbiani Alessandra , Scandurra Valeria , Canitano Roberto , Mencarelli Maria Antonietta , Renieri Alessandra , Ariani Francesca
Published: April 2022

Genetic defects in the SHANK2 gene, encoding for synaptic scaffolding protein, are associated with a variety of neurodevelopmental conditions, including autism spectrum disorders and mild to moderate intellectual disability. Until now, limited patient clinical descriptions have been published. Only 13 unrelated patients with SHANK2 pathogenic variations or microdeletions have been reported worldwide. By Exome Sequencing, we identified a de novo stop-gain variant, c.334C>T, p.(Gln112*), in an Italian patient with a neurodevelopmental disorder. The patient (9 years old) presented the following facial features: a flat profile, thick eyebrows, long eyelashes, a bulbous nasal tip and a prominent columella, retracted ears, dental anomalies. The patient showed speech delay and mild neuromotor delay but not autism spectrum disorder. In conclusion, this patient with a novel pathogenic variant in SHANK2 enlarges the phenotypic spectrum of SHANK2-mutated patients and demonstrates that the severity of SHANK2-associated disorders is highly variable.
Oncogenic Role of Connective Tissue Growth Factor Is Associated with Canonical TGF-β Cascade in Colorectal Cancer
Authors: Hosseini Shaghayegh , Rejali Leili , Pezeshkian Zahra , Malekian Mahtash , Fatemi Nayeralsadat , Peyravian Noshad , Hadizadeh Mahrooyeh , Mohsenifar Zhaleh , Khanabadi Binazir , Farzam Maral , Sherkat Ghazal , Asadzadeh Aghdaei Hamid , Nazemalhosseini Mojarad Ehsan , Ashrafian Bonab Maziar
Published: April 2022

TGF-β signaling pathways promote tumour development and control several downstream genes such as CTGF and MMPs. This study aimed to investigate the association between CTGF and MMP-1 mRNA expressions with clinicopathological status and survival rate in colorectal cancer patients. We investigated expression levels of CTGF and MMP-1 genes in paraffin-embedded tumours and adjacent normal tissue blocks (ADJ) by Real Time-PCR. Then, the expression of Smad2 and Smad4 proteins in the TGF-β canonical pathway was evaluated by immunohistochemistry. Finally, the correlation between CTGF, MMP-1, and the canonical TGF-β-signalling pathway with the clinicopathological features was investigated. Expression levels of MMP-1and CTGF were higher in tumours compared with adjacent normal tissues. Overexpression levels of MMP-1 and CTGF were associated with lymph node metastasis, distant metastasis, tumour histopathological grading, advanced stage, and poor survival (p < 0.05). Additionally, a significant association between the upregulation of MMP-1 and tumour location was noted. Upregulation of Smad2 and Smad4 proteins were also significantly correlated with lymph node metastasis, distant metastasis, advanced stage, and poor survival (p < 0.0001). This study showed that canonical TGF-β signalling regulates both CTGF and MMP-1 expression and CRC progression. Moreover, TGF-β signalling and its downstream genes could be used as novel biomarkers and novel approaches for targeted therapy in CRC.
Comparative Genomic Hybridization to Microarrays in Fetuses with High-Risk Prenatal Indications: Polish Experience with 7400 Pregnancies
Authors: Kowalczyk Katarzyna , Bartnik-Głaska Magdalena , Smyk Marta , Plaskota Izabela , Bernaciak Joanna , Kędzior Marta , Wiśniowiecka-Kowalnik Barbara , Deperas Marta , Domaradzka Justyna , Łuszczek Alicja , Dutkiewicz Daria , Kozar Agata , Grad Dominika , Niemiec Magdalena , Ziemkiewicz Kamila , Magdziak Róża , Braun-Walicka Natalia , Barczyk Artur , Geremek Maciej , Castañeda Jennifer , Kutkowska-Kaźmierczak Anna , Własienko Paweł , Jakubów-Durska Krystyna , Dębska Marzena , Kucińska-Chahwan Anna , Kozłowski Szymon , Mikulska Boyana , Issat Tadeusz , Roszkowski Tomasz , Nawara-Baran Agnieszka , Runge Agata , Jakubiuk-Tomaszuk Anna , Kruczek Anna , Kostyk Ewa , Pietras Grzegorz , Limon Janusz , Zwoliński Jerzy , Ochman Karolina , Szajner Tomasz , Węgrzyn Piotr , Wielgoś Mirosław , Sąsiadek Maria , Obersztyn Ewa , Nowakowska Beata Anna
Published: April 2022

The aim of this study was to determine the suitability of the comparative genomic hybridization to microarray (aCGH) technique for prenatal diagnosis, but also to assess the frequency of chromosomal aberrations that may lead to fetal malformations but are not included in the diagnostic report. We present the results of the aCGH in a cohort of 7400 prenatal cases, indicated for invasive testing due to ultrasound abnormalities, high-risk for serum screening, thickened nuchal translucency, family history of genetic abnormalities or congenital abnormalities, and advanced maternal age (AMA). The overall chromosomal aberration detection rate was 27.2% (2010/7400), including 71.2% (1431/2010) of numerical aberrations and 28.8% (579/2010) of structural aberrations. Additionally, the detection rate of clinically significant copy number variants (CNVs) was 6.8% (505/7400) and 0.7% (57/7400) for variants of unknown clinical significance. The detection rate of clinically significant submicroscopic CNVs was 7.9% (334/4204) for fetuses with structural anomalies, 5.4% (18/336) in AMA, 3.1% (22/713) in the group of abnormal serum screening and 6.1% (131/2147) in other indications. Using the aCGH method, it was possible to assess the frequency of pathogenic chromosomal aberrations, of likely pathogenic and of uncertain clinical significance, in the groups of cases with different indications for an invasive test.
MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection
Authors: Zhou Yingchao , Zha Lingfeng , Wu Jianfei , Wang Mengru , Zhou Mengchen , Wu Gang , Cheng Xiang , Huang Zhengrong , Xie Qiang , Tu Xin
Published: April 2022

Aortic dissection (AD) is a life-threatening disease with high morbidity and mortality, and effective pharmacotherapeutic remedies for it are lacking. Therefore, AD’s molecular pathogenesis and etiology must be elucidated. The aim of this study was to investigate the possible mechanism of mediator complex subunit 12 (human: MED12, mouse: Med12)involvement in AD. Firstly, we examined the expression of MED12 protein (human: MED12, mouse: Med12) in the aortic tissues of AD patients and AD mice. Subsequently, Med12 gene silencing was accomplished with RNA interference (siRNA). The effects of Med12 on AD and the possible biological mechanisms were investigated based on the proliferation, senescence, phenotypic transformation, and its involved signal pathway of mouse aortic smooth muscle cells (MOVAS), s. The results show that the expression of MED12 in the aortae of AD patients and AD mice was decreased. Moreover, the downregulation of Med12 inhibited the proliferation of MOVAS and promoted senescence. Further research found that Med12, as an inhibitor of the TGFβ1 signaling pathway, reduced the expression of Med12 and enhanced the activity of the TGFβ1 nonclassical signaling pathway, while TGFβ1 inhibited the phenotype transformation and proliferation of MOVAS by inhibiting Med12 synthesis. In conclusion, Med12 affected the phenotype, proliferation, and senescence of MOVAS through the TGFβ signaling pathway. This study provides a potential new target for the prevention and treatment of AD.
Expanding the Phenotype of B3GALNT2-Related Disorders
Authors: D’haenens Erika , Vergult Sarah , Menten Björn , Dheedene Annelies , Kooy R. Frank , Callewaert Bert
Published: April 2022

Dystroglycanopathies are a group of congenital muscular dystrophies (CMDs) that include a broad phenotypic spectrum ranging from late-onset limb-girdle muscular dystrophy to severe muscle–eye–brain disease, Walker–Warburg syndrome, and Fukuyama congenital muscular dystrophy. In addition to clinical heterogeneity, CMDs are characterized by genetic heterogeneity. To date, 18 genes have been associated with CMDs. One of them is B3GALNT2, which encodes the β-1,3-N-acetylgalactosaminyltransferase 2 that glycosylates α-dystroglycan. In this study, using exome sequencing, we identify a homozygous frameshift variant in B3GALNT2 due to a mixed uniparental disomy of chromosome 1 in a 7-year-old girl with global developmental delay, severely delayed active language development, and autism spectrum disorder but without any symptoms of muscular dystrophy. In addition to this case, we also provide an overview of all previously reported cases, further expanding the phenotypic spectrum.
Schizophrenia and Bipolar Polygenic Risk Scores in Relation to Intracranial Volume
Authors: de Zwarte Sonja M. C. , Brouwer Rachel M. , Kahn René S. , van Haren Neeltje E. M.
Published: April 2022

Schizophrenia and bipolar disorder are neurodevelopmental disorders with overlapping symptoms and a shared genetic background. Deviations in intracranial volume (ICV)—a marker for neurodevelopment—differ between schizophrenia and bipolar disorder. Here, we investigated whether genetic risk for schizophrenia and bipolar disorder is related to ICV in the general population by using the UK Biobank data (n = 20,196). Polygenic risk scores for schizophrenia (SZ-PRS) and bipolar disorder (BD-PRS) were computed for 12 genome wide association study P-value thresholds (PT) for each individual and correlations with ICV were investigated. Partial correlations were performed at each PT to investigate whether disease specific genetic risk variants for schizophrenia and bipolar disorder show different relationships with ICV. ICV showed a negative correlation with SZ-PRS at PT ≥ 0.005 (r < −0.02, p < 0.005). ICV was not associated with BD-PRS; however, a positive correlation between BD-PRS and ICV at PT = 0.2 and PT = 0.4 (r = +0.02, p < 0.005) appeared when the genetic overlap between schizophrenia and bipolar disorder was accounted for. Despite small effect sizes, a higher load of schizophrenia risk genes is associated with a smaller ICV in the general population, while risk genes specific for bipolar disorder are correlated with a larger ICV. These findings suggest that schizophrenia and bipolar disorder risk genes, when accounting for the genetic overlap between both disorders, have opposite effects on early brain development.
Future with a Past: Future Scenarios of Development in Yucatan in ¿Qué les pasó a las abejas?
Authors: Beilin Kata
Published: April 2022

Since the Green Revolution, the development of agriculture has been measured by the relation between the chemical input (fertilizers and pesticides) and yield. Other factors, such as deforestation, water pollution, biodiversity loss and the loss of human health, were not part of these calculations. With the advent of genetically modified monocrops in the 1990s, GM soy in particular, plantations took over larger surfaces of land, accelerating these negative processes on a previously unknown scale. It has become clear that if this type of agriculture persists, toxic plantations will soon consume the planet. One of the phenomena prompting this awareness in different places of the world was the death of bees. ¿Qué les pasó a las abejas?, directed by Adriana Otero and Robín Canul, relates the environmental conflict between GM soy growers in the Yucatan Peninsula, Mexico, and Mayan beekeepers. Not long after the arrival of GM soy to Yucatan, the bees began to die. When their honey was rejected by the EU authorities due to contamination with transgenic pollen, Mayan beekeepers realized that not only their bees, but also their water and their bodies were poisoned by GM soy agriculture, while their forests were cut for new plantations. The Maya demanded that the state prohibit the planting of GM soy on their land. ¿Qué les pasó a las abejas? is a character-driven documentary featuring leaders of the Maya beekeepers’ movement, including the recipient of the Goldman Environmental Prize 2020, Leydy Pech. Maya Land; Listening to the Bees, my own documentary, reflects on the same environmental conflict and asks what the future would look like if bee health was considered a criterion of sustainable development. A vision of an alternative future emerges in both films through a series of interviews with Mayan beekeepers, scientists, and policy makers; bees are healthy, water is clean, and agriculture incorporates a mixture of ancient techniques and cutting-edge technologies that assist humans in rethinking their relationships with land and plants.
Imagining the Blitz and Its Aftermath: The Narrative Performance of Trauma in Sarah Waters’s The Night Watch
Authors: Onega Susana
Published: April 2022

Critics agree that Sarah Waters’ fourth novel, The Night Watch (2006), marks a turn in her fiction, away from the farcical tone of her first three neo-Victorian novels and towards an ever-more serious concern with the changes in class structures and gender roles brought about by the fact of war. The novel tells the parallel stories of three women and one man living in various areas of London in the 1940s. Though they have different social status, ideology, and sexual orientation, they share similarly traumatic experiences as, together with war trauma, they harbour individual feelings of loss and/or shame related to their deviance from patriarchal norms. The article seeks to demonstrate that the palimpsestic and backward structure of the novel performs formally the ‘belatedness of trauma’ (Caruth 1995, pp. 4–5), in an attempt to respond aesthetically and ethically to the ‘mnemonic void’ (Freud [1014] 1950) or ‘black hole’ (Pitman and Orr 1990; Bloom 2010; Van der Kolk and McFarlane [1998] 2004) left both in the characters’ traumatised psyches and in our cultural memory of the 1940s by the erased memories of the decade’s non-normative or dissident others.
Comment on Patel, B.; Makaryus, A.N. Artificial Intelligence Advances in the World of Cardiovascular Imaging. Healthcare 2022, 10, 154
Authors: Giansanti Daniele
Published: April 2022

Regarding Dr. Makaryus’s interesting review study [...]
Relationship between Associated Neuropsychological Factors and Fall Risk Factors in Community-Dwelling Elderly
Authors: Yi DongHyun , Jang SeungJun , Yim JongEun
Published: April 2022

This study examined whether neuropsychological factors could affect fall risk factors in the community-dwelling elderly via correlation analysis. A total of 393 older adults (76.69 ± 6.01) participated in this study. Cognitive function, depression, fall efficacy, balance confidence, balance, gait, and muscle strength were evaluated, and the correlation between psychological factors and fall risk factors was analyzed. Additionally, a multiple regression analysis was conducted to determine whether or not there was a significant effect between psychological factors and fall risk factors. Analysis showed that the psychological factors examined were all significantly correlated with the fall risk factors. A correlation analysis between cognitive function and fall risk factors showed that the correlation coefficient of the 6-Meter Walk Test was highest; for depression and fall risk factors, the correlation coefficient of gait speed was highest; for fall efficacy and fall risk factors, the correlation coefficient of the 6-Meter Walk Test was highest; and for confidence in balancing and fall risk factors, the correlation coefficient of the 6-Meter Walk Test was highest. This study suggests that psychological factors affect fall risk factors in the community-dwelling elderly, and a multifaceted approach that includes psychological factors would be helpful in providing interventions for falls.
Determinants and Factors Associated with the Maintenance of Exclusive Breastfeeding after Hospital Discharge after Birth
Authors: Martínez-Vázquez Sergio , Hernández-Martínez Antonio , Rodríguez-Almagro Julián , Peinado-Molina Rocío Adriana , Martínez-Galiano Juan Miguel
Published: April 2022

The benefits of exclusive breastfeeding are well known for both mother and baby. Despite this, rates of exclusive breastfeeding remain low. The present study aimed to determine the factors associated with the maintenance of this type of feeding after being discharged from the hospital after childbirth. A cross-sectional study was carried out with 1200 postpartum women in Spain. Sociodemographic, obstetric, and neonatal data were collected. Odds ratios (OR) and adjusted odds ratios (aOR) with 95% confidence intervals were calculated. Early breastfeeding initiation was identified as a factor that favors breastfeeding after hospital discharge (aOR: 2.47; 95%CI: 1.77, 3.45). Other factors that favor breastfeeding after discharge included the woman feeling very supported by her partner during pregnancy, childbirth, and the puerperium (aOR: 2.54; 95%CI:1.30, 5.00) and having previously breastfed other children (aOR: 1.97; 95%CI: 1.40, 2.76). Among the factors that hindered exclusive breastfeeding after discharge were multiple or twin pregnancies (aOR: 0.31; 95%CI 0.12, 0.83), induction of labor (aOR: 0.73; 95%CI: 0.53, 0.99), admission of the newborn to the neonatal intensive care unit (NICU) (aOR: 0.31; 95%CI 0.19, 0.52), using epidural pain relief during labor (aOR: 0.41; 95%CI: 0.27, 0.64), or a preterm newborn (aOR: 0.38; 95%CI: 0.21, 0.69). For all these reasons, it is essential to promote certain practices such as the early start of breastfeeding or the induced onset of labor, among others, in order to promote the maintenance of exclusive breastfeeding beyond hospital discharge after childbirth.
PpMYB39 Activates PpDFR to Modulate Anthocyanin Biosynthesis during Peach Fruit Maturation
Authors: Khan Irshad Ahmad , Rahman Masood Ur , Sakhi Shazia , Nawaz Ghazala , Khan Aftab Ahmad , Ahmad Tanveer , Adnan Mohammad , Khan Shah Masaud
Published: April 2022

Anthocyanins are a class of water-soluble flavonoids widely present in fruits and vegetables responsible for the red flesh formation of peach fruit. Previously, several genes of the MYB family have been reported as transcriptional regulators of the anthocyanin biosynthetic pathway of structural genes in plants. In this study, through comparative transcriptome analysis of the white and red flesh peach cultivars of Harrow Blood and Asama Hakuto, a predicted transcription factor of the R2R3MYB family, PpMYB39, was identified to be associated with anthocyanin biosynthesis in peach fruit. In red-fleshed peach cultivars, the maximum amount of anthocyanin accumulated 95 days after full bloom (DAFB), at full maturity near ripening. Our results showed that, at this stage, PpMYB39 had the highest expression level among the 13 differentially expressed genes (DEGs) found in both red- and white-fleshed fruits, as well as a high correlation with total anthocyanin content throughout fruit development. Moreover, the expression analysis of the structural genes of the anthocyanin biosynthetic pathway in peach fruit revealed that Prunus persica Dihydroflavonol-4-reductase (PpDFR) was co-expressed and up-regulated with PpMYB39 at 95 DAFB, suggesting its possible role as a transcriptional activator of MYB39. This was further confirmed by a yeast one-hybrid assay and a dual luciferase reporter assay. Our results will be helpful in the breeding of peach cultivars and the identification and significance of color in peaches and related fruit species, in addition to providing an understanding of color formation in peach fruit for future research.
Potential Uses of Scallop Shell Powder as a Substrate for the Cultivation of King Oyster Mushroom (Pleurotus eryngii)
Authors: Zhou Yuanyuan , Li Zihao , Zhang Haijun , Hu Qingxiu , Zou Yajie
Published: April 2022

Scallop shells are currently a major form of waste generated in the Chinese fishing industry. However, they have the potential to be used as important industrial products. This study was conducted to assess the utility of scallop shell powder (SSP) supplementation in improving the growth of king oyster mushrooms (Pleurotus eryngii) grown on sawdust and sugarcane bagasse substrates. The outcomes of interest included mycelial growth, yield, biological efficiency, fruiting body traits, nutrient supply, and the mineral composition of P. eryngii. Supplementation with SSP accelerated the mycelial growth of P. eryngii. The yield of fruiting bodies (399.5 g/bag) and the biological efficiency (84.6%) were 14% higher after supplementation of the substrate with 2% SSP compared with those of mushrooms grown on substrates not supplemented with SSP (349.8 g/bag and 74.0%, respectively). Moreover, the crude protein and fiber contents of the fruiting bodies significantly improved after growth with SSP. Furthermore, supplementation with 2% SSP increased the calcium (Ca) content of the fruiting bodies of P. eryngii by 64% (to 67.2 ± 15.7 mg kg−1) compared with that of mushrooms grown on a control substrate (41.0 mg kg−1). This study revealed that P. eryngii can efficiently use the Ca provided by raw SSP, generating higher Ca contents in their fruiting bodies. Our results demonstrate that the supplementation of substrates with SSP can be useful for enhancing both the yield and nutritional content of P. eryngii.
A Water Balancing Act: Water Balances Highlight the Benefits of Community-Based Adaptive Management in Northern New Mexico, USA
Authors: Conrad Lily M. , Fernald Alexander G. , Guldan Steven J. , Ochoa Carlos G.
Published: April 2022

Quantifying groundwater recharge from irrigation in water-scarce regions is critical for sustainable water management in an era of decreasing surface water deliveries and increasing reliance on groundwater pumping. Through a water balance approach, our study estimated deep percolation (DP) and characterized surface water and groundwater interactions of two flood-irrigated fields in northern New Mexico to evaluate the regional importance of irrigation-related recharge in the context of climate change. DP was estimated for each irrigation event from precipitation, irrigation input, runoff, change in soil water storage, and evapotranspiration data for both fields. Both fields exhibited positive, statistically significant relationships between DP and total water applied (TWA), where one field exhibited positive, statistically significant relationships between DP and groundwater level fluctuation (GWLF) and between GWLF and total water applied. In 2021, total DP on Field 1 was 739 mm, where 68% of irrigation water applied contributed to DP. Field 2′s total DP was 1249 mm, where 81% of irrigation water applied contributed to DP. Results from this study combined with long-term research indicate that the groundwater recharge and flexible management associated with traditional, community-based irrigation systems are the exact benefits needed for appropriate climate change adaptation.
Computational Modeling of Therapy with the NMDA Antagonist in Neurodegenerative Disease: Information Theory in the Mechanism of Action of Memantine
Authors: Świetlik Dariusz , Kusiak Aida , Ossowska Agata
Published: April 2022

(1) Background: in patients with neurodegenerative diseases, noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonists provide neuroprotective advantages. We performed memantine therapy and proved mathematical and computer modeling of neurodegenerative disease in this study. (2) Methods: a computer simulation environment of the N-methyl-D-aspartate receptor incorporating biological mechanisms of channel activation by high extracellular glutamic acid concentration. In comparison to controls, pathological models were essentially treated with doses of memantine 3–30 µM. (3) Results: the mean values and 95% CI for Shannon entropy in Alzheimer’s disease (AD) and memantine treatment models were 1.760 (95% CI, 1.704–1.818) vs. 2.385 (95% CI, 2.280–2.490). The Shannon entropy was significantly higher in the memantine treatment model relative to AD model (p = 0.0162). The mean values and 95% CI for the positive Lyapunov exponent in AD and memantine treatment models were 0.125 (95% CI, NE–NE) vs. 0.058 (95% CI, 0.044–0.073). The positive Lyapunov exponent was significantly higher in the AD model relative to the memantine treatment model (p = 0.0091). The mean values and 95% CI for transfer entropy in AD and memantine treatment models were 0.081 (95% CI, 0.048–0.114) vs. 0.040 (95% CI, 0.019–0.062). The transfer entropy was significantly higher in the AD model relative to the memantine treatment model (p = 0.0146). A correlation analysis showed positive and statistically significant correlations of the memantine concentrations and the positive Lyapunov exponent (correlation coefficient R = 0.87, p = 0.0023) and transfer entropy (TE) (correlation coefficient R = 0.99, p < 0.000001). (4) Conclusions: information theory results of simulation studies show that the NMDA antagonist, memantine, causes neuroprotective benefits in patients with AD. Our simulation study opens up remarkable new scenarios in which a medical product, drug, or device, can be developed and tested for efficacy based on parameters of information theory.
Exploring the Effect of Health on Migrants’ Social Integration in China
Authors: Kang Xiang , Du Mingxi , Wang Siqin , Du Haifeng
Published: April 2022

There are 376 million migrants, which account for more than 25% of the population in China according to the newest national demographic census, most of whom are from undeveloped areas to developed urban regions. Migrants’ social integration was one of the most important issues when the country aimed to build an inclusive society. As a form of human capital, the effect of migrants’ health status on social integration has rarely been explored until now, especially empirically. Previous studies have usually ignored health indicators when discussing the determinants of migrants’ social integration, and understanding the role of migrants’ physical health and mental health on their social integration is significant for efforts to ensure inclusive urbanization. For filling this research gap, the China Migrants Dynamic Survey dataset was used to uncover the role of migrants’ health status, including physical health and mental health, in their degree of social integration, and a further comparison of impact was conducted among rural–urban and urban–urban migrants. Through the empirical analysis, our results indicated the following. First, both better physical and mental health lead to higher social integration levels, and a one-point increase in physical and mental health improves the odds of good social integration by 33.27% and 5.98% for belonging and 66.05% and 6.35% for harmony, respectively. Second, health status is equally important for rural–urban and urban–urban migrants’ social integration, and the significant positive effect was consistent across groups, although some other impact factors may exhibit differences. Third, the effect of health status on social integration was moderated by social participation, which was more obvious for mental health than physical health. According to our findings, we discuss the measures to promote migrants’ health status and additional countermeasures to improve their social integration level.
Human Excreta and Food Waste of a Typical Rural Area in China: Characteristics and Co-Fermentation
Authors: Kinhoun Jean Joël Roland , Li Ao , Lv Minghuan , Shi Yunpeng , Fan Bin , Qian Tingting
Published: April 2022

Human excreta (HE) and food waste (FW) are the primary contaminants in rural regions. Prior to treating these contaminants, mastering their properties is required. In this study, the characteristics of the HE leaving the body and FW leaving the kitchen to the subsequent respective fermentation were studied. Moreover, two kinds of co-fermentation processes for HE and FW were also investigated on the basis of mastering the properties. The results showed that, for a healthy adult, fresh feces, urine, and FW produced were about 163 g/cap/d (57.3 gCOD/cap/d), 1.6 L/cap/d (6.7 gN/cap/d), and 250 g/cap/d (35.0 gCOD/cap/d), respectively. In HE, about 75% of nitrogen and phosphorus were contained in urine. It takes at least three days for crushed FW discharged via water flushing to settle completely, and the COD removal efficiency after precipitation was around 75%. Mixing HE with FW after discharge, i.e., the initial unit of the process was 20% more efficient in fermentation than mixing after the respective pre-fermentation. This paper presents the characteristics of HE and FW and provides the optimized co-fermentation process, which provides technical support for the realization of environmental sanitation in rural areas.
Microbial Degradation of Azo Dyes: Approaches and Prospects for a Hazard-Free Conversion by Microorganisms
Authors: Ngo Anna Christina R. , Tischler Dirk
Published: April 2022

Azo dyes have become a staple in various industries, as colors play an important role in consumer choices. However, these dyes pose various health and environmental risks. Although different wastewater treatments are available, the search for more eco-friendly options persists. Bioremediation utilizing microorganisms has been of great interest to researchers and industries, as the transition toward greener solutions has become more in demand through the years. This review tackles the health and environmental repercussions of azo dyes and its metabolites, available biological approaches to eliminate such dyes from the environment with a focus on the use of different microorganisms, enzymes that are involved in the degradation of azo dyes, and recent trends that could be applied for the treatment of azo dyes.
A Review of Urban Microclimate Research Based on CiteSpace and VOSviewer Analysis
Authors: Li Jiajing , Mao Yang , Ouyang Jingyi , Zheng Shuanning
Published: April 2022

Urban microclimate has a direct impact on the quality of life of urban residents. Therefore, research on urban microclimates has received greater attention from contemporary scholars. At present, there is a lack of quantitative summary and review of the research in the field of urban microclimate, and it is urgent to sort out its research context and evolution. The Web of Science was used as the data source, and CiteSpace and VOSviewer software were used to analyze the urban microclimate research from 1980 to 2020. We discussed the annual trends, research countries, research institutions, key authors, highly cited publications, hot issues, and research fronts. The study found that: (1) the number of published articles on urban microclimate has experienced three stages: initial stage—slow growth period—rapid growth period; (2) European and American countries were the first to focus on urban microclimate research, while China started late but developed rapidly; (3) the research topics of urban microclimate are thermal comfort, improvement strategies, urban street canyons, and urban heat island effect; (4) the frontiers of urban microclimate include research on urban microclimate and building energy, ecosystem services, and urban parks.
Saharan Dust and Childhood Respiratory Symptoms in Benin
Authors: McElroy Sara , Dimitrova Anna , Evan Amato , Benmarhnia Tarik
Published: April 2022

Mineral dust is one of the largest natural constituents of coarse particulate matter (PM10). Most of these dust emissions originate from northern Africa, and several hundred tera-grams of dust are emitted annually from this region. Previous evidence has linked dust PM10 to adverse respiratory outcomes in children. However, most of these studies have been from high-income countries (HICs) or examined dust from other regions of the world, mainly Asia. Evidence from low-to-middle-income countries (LMICs) in Africa is scarce. Respiratory infections are one of the leading causes of under-five mortality across the globe. However, there is a poignant disparity in studies examining these outcomes in children in the region where most dust is emitted. This study linked remotely sensed satellite data to a nationally representative survey to examine acute exposure to dust in children living in Benin using a time-stratified case-crossover analysis. We identified acute effects of exposure to dust and increased risk of cough in children under five. The effect of increased risk is strongest within two weeks of exposure and dissipates by four weeks. Children living in rural areas and households with lower income had a greater risk of adverse respiratory outcomes when exposed to dust. We could elucidate the specific period and conditions of increased risk for respiratory problems in children living in Benin.
Measuring Environmental Justice in Real Time: A Pilot Study Using Digital Participatory Method in the Global South, Nepal
Authors: Shrestha Rehana , Telkmann Klaus , Schüz Benjamin , Koju Pramesh , Shrestha Reshma , Karmacharya Biraj , Bolte Gabriele
Published: April 2022

Individuals’ perceived fairness or justice beliefs are related to health in numerous ways. However, environment justice research to date has given little attention to perceived fairness of environmental exposures as experienced by individuals. This study explored the feasibility of a bottom-up digital participatory (via mobile phones) approach using ecological momentary assessment (EMA) to capture individuals’ subjective experience of environmental exposures and the subjective evaluation of fairness by those affected in the context of Nepal. In total, 22 individuals participated in the study for 28 days. The results show high rates of study retention and adherence. Individuals’ justice perception was found to vary within and between individuals, but also substantially depending on the types of environmental exposures. Nevertheless, the study indicates that uncertainties are inevitable as study design and timing may conflict participants’ daily lives and priorities. The method allows us to consider multiple geographic contexts of individuals’ everyday lives beyond residential environment. This pilot study proved the possibility to assess perceptions of environmental justice issues and demonstrated the necessary steps to using digital participatory method for assessing subjective perception of fairness of individuals.
International Travel as a Risk Factor for Carriage of Extended-Spectrum β-Lactamase-Producing Escherichia coli in a Large Sample of European Individuals—The AWARE Study
Authors: Rodríguez-Molina Daloha , Berglund Fanny , Blaak Hetty , Flach Carl-Fredrik , Kemper Merel , Marutescu Luminita , Pircalabioru Gradisteanu Gratiela , Popa Marcela , Spießberger Beate , Wengenroth Laura , Chifiriuc Mariana Carmen , Larsson D. G. Joakim , Nowak Dennis , Radon Katja , de Roda Husman Ana Maria , Wieser Andreas , Schmitt Heike
Published: April 2022

Antibiotic resistance (AR) is currently a major threat to global health, calling for a One Health approach to be properly understood, monitored, tackled, and managed. Potential risk factors for AR are often studied in specific high-risk populations, but are still poorly understood in the general population. Our aim was to explore, describe, and characterize potential risk factors for carriage of Extended-Spectrum Beta-Lactamase-resistant Escherichia coli (ESBL-EC) in a large sample of European individuals aged between 16 and 67 years recruited from the general population in Southern Germany, the Netherlands, and Romania. Questionnaire and stool sample collection for this cross-sectional study took place from September 2018 to March 2020. Selected cultures of participants’ stool samples were analyzed for detection of ESBL-EC. A total of 1183 participants were included in the analyses: 333 from Germany, 689 from the Netherlands, and 161 from Romania. Travels to Northern Africa (adjusted Odds Ratio, aOR 4.03, 95% Confidence Interval, CI 1.67–9.68), Sub-Saharan Africa (aOR 4.60, 95% CI 1.60–13.26), and Asia (aOR 4.08, 95% CI 1.97–8.43) were identified as independent risk factors for carriage of ESBL-EC. Therefore, travel to these regions should continue to be routinely asked about by clinical practitioners as possible risk factors when considering antibiotic therapy.
Quantifying the Ecosystem Services of Soda Saline-Alkali Grasslands in Western Jilin Province, NE China
Authors: Chang Lei , Zhao Zhibo , Jiang Lixin , Li Yuefen
Published: April 2022

This study aimed to quantitatively describe the ecosystem services of soda saline-alkali grasslands based on literature research, the InVEST model, a transition matrix, and Spearman’s correlation analysis. The chosen methodology could provide insight into the relationships between different services to provide empirical evidence for decision-making concerning the protection and restoration of saline-alkali grasslands. The research provided several insights into the ecological situation in western Jilin Province. First, the area of saline-alkali grassland in western Jilin Province had noticeably decreased from 1990 to 2018. Moreover, the threat of grassland degradation in western Jilin Province has increased year by year, and has become the main problem facing the ecological environment of this region. Second, the results demonstrated how the amount of grassland area, and coverage, are intricately linked to the provided ecosystem services, and maintaining the stability of ecosystem services is the basis for future efforts to increase grassland area and coverage. A trade-off relationship exists between water supply services and other ecosystem services, which indirectly confirms a climatic cause for grassland salinization in western Jilin Province. The analyses identified various types of grassland ecosystem service hotspots, but the share of hotspots representing all four assessed ecosystem services was small; this indicates that the grassland ecosystem of western Jilin Province is of generally poor quality. In conclusion, increasing grassland salinization has reduced vegetation coverage, which leads to the degradation of the grassland ecosystem and, in turn, affects the relationships between various ecosystem services.
Surgery Is in Itself a Risk Factor for the Patient
Authors: Aranaz-Ostáriz Verónica , Gea-Velázquez De Castro María Teresa , López-Rodríguez-Arias Francisco , San José-Saras Diego , Vicente-Guijarro Jorge , Pardo-Hernández Alberto , Aranaz-Andrés Jesús María ,   on behalf of the ESHMAD Director Group and External Advisers
Published: April 2022

(1) Background: Adverse events (AE) affect about 1 in 10 hospitalised patients, and almost half are related to surgical care. The aim of this study is to determine the prevalence of AE in operated and non-operated patients in surgical departments in order to determine whether surgical treatment is a risk factor for AE. (2) Methods: A cross-sectional design that included 3123 patients of 34 public hospitals in the Community of Madrid determining the prevalence of AEs in operated and non-operated patients in surgical departments. (3) Results: The prevalence of AE in non-operated patients was 8.7% and in those operated was 15.8%. The frequency of AE was higher in emergency surgery (20.6% vs. 12.4%). The 48.3% of AEs led to an increase in hospital stay, and surgery was involved in 92.4% of cases. The most frequent AEs were related to hospital-acquired infection (42.63%), followed by those related to a procedure (37.72%). In the multivariate analysis, being operated on represented 2.3 times the risk of developing an AE. (4) Conclusions: Surgical sites are particularly vulnerable to AE. Surgical intervention alone is a risk factor for AE, and we must continue to work to improve the safety of both patient care and the working environment of surgical professionals.
Interventions to Improve Body Composition, Upper and Lower Extremity Muscle Strength, and Balance Ability of Older Female Adults: An Intervention Study
Authors: Huang Wei-Yang , Wu Cheng-En
Published: April 2022

The aim of the present study was to understand the effects of a moderate-intensity physical activity program on the changes observed in the body composition, upper and lower extremity muscle strength, as well as balance in elderly female adults in order to evaluate sarcopenia. In this study, 30 healthy elderly females were recruited and were randomly assigned to either the control group or the experimental group. The experimental group engaged in a moderate-intensity physical activity program twice a week for 8 weeks. Using a body composition analyzer, the senior fitness test, and handgrip strength and gait speed tests, all participants were tested in pre- and post-tests. The results of the study revealed changes in the overall body composition in the experimental group, with significant decreases in body mass index, body fat percentage, and body fat mass and substantial increases in the basal metabolic rate and skeletal muscle mass, while the upper and lower extremity muscle strength and balance ability also showed significant improvements. The moderate-intensity physical activity program also increased upper limb handgrip strength and lower limb gait speed, showing that the plan was able to effectively evaluate sarcopenia. The study concluded that using upper limb handgrip strength and lower limb walking speed to evaluate sarcopenia are useful diagnostic tools. Moderate-intensity physical activity is effective for improving muscle strength and reducing sarcopenia.