bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation the effect of amitriptyline and/or ashwagandha on body weight in male rats
Authors: Taqa Ghada A. , Idrees Ismail  R.
Published: January 2024

study aims to examine and compare the effects of amitriptyline and ashwagandha on body weight in male rats by measurement weight for 4 weeks after their exposure to these two compound. Materials and Methods: This study used twenty healthy adult male albino rats that were 8-10 weeks old and weighed 200-250g. The animals were randomly divided to 4 groups (n= 5 /group), Group 1:  were served as a control group received distilled water orally (1.0ml/kg) group 2: were administered amitriptyline (10mg/kg/ orally) group 3: were administered (200mg/kg) of ashwagandha root extract and group 4: were co-administered amitriptyline (10mg/kg) and ashwagandha (200mg/kg). After 30 days of administration, The body weight was measured every week starting from the first day of administration to last week of experimental for all rats. Results: According to the  one-way analysis of variance(ANOVA) showed significant differences in growth value in the co-administered Amitriptyline and ashwagandha group ( 1.48±0.09) significantly  increased in comparison with control group (1.27 ± 0.04) at week 4, otherwise, there is no significant differences in growth among groups at other  periods at p value ≤0.05. Conclusion: Amitriptyline induced oxidative stress causing increased in body weight during experimental periods. Administration ashwagandha alone non-significant effect on body weight but when co-administration with amitriptyline significantly increase in body weight.
PENGARUH MEDIA ULAR TANGGA PADA PEMBELAJARAN MENULIS TEKS EKSPLANASI OLEH SISWA KELAS VIII SMP NEGERI 4 KUTACANE TAHUN PEMBELAJARAN 2022/2023
Authors: Mayang Rina  , Irfan Johari  , Septian Azwar
Published: January 2024

Penelitian ini bertujuan untuk mengetahui penengaruh media ular tangga pada pembelajaran menulis teks eksplanasi oleh siswa kelas VIII SMP Negeri 4 Kutacane Tahun Pembelajaran 2022/2023. Adapun yang menjadi populasi dalam penelitian ini adalah seluruh siswa kelas VIII yang berjumlah 81 orang dan sampel berjumlah 30 orang. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan rancangan one group pretest and postest design dengan analisis data menggunakan uji “t”. Dari pengelolahan data di peroleh nilai rata-rata tes awal pembelajaran menulis teks eksplanasi oleh siswa sebesar 55 dan termasuk dalam kategori kurang, dengan standar deviasinya sebesar 5,72 dan mengalami perubahan nilai hasil tes akhirnya dengan nilai rata-rata sebesar 78,67 dan termasuk dalam kategori baik, dengan standard deviasinya sebesar 4,14. Berdasarkan hasil nilai tes awal dan tes akhir siswa tersebut dan jika di kaitkan dengan nilai KKM mata pelajaran bahasa Indonesia untuk SMP sebesar 70 maka pembelajaran menulis teks eksplanasi siswa termasuk dalam kategori baik. Dari pengujian hipotesis diperoleh nilai t hitung = 8,01 dan di konsultasikan dengan nilai t_tabel pada taraf signifikan 5% = 2,045 dengan demikian t_hitung> t_tabel  atau 8,01> 2,045 maka hipotesis di terima. Jadi dapat disimpulkan bahwa ada pengaruh media ular tangga pada pembelajaran menulis teks eksplanasi oleh siswa kelas VIII SMP Negeri 4 Kutacane Tahun Pembelajaran 2022/2023.
THE EFFECTIVENESS OF PAIR CHECK METHOD IN WRITING ANNOUNCEMENT TEXT AT CLASS X SMA SWASTA RAUDHATUL HASANAH ACADEMIC YEAR 2021/2022
Authors: Fauziah Ajmi  , Sabrun Jamil  , ABDUL HALIM
Published: January 2024

This study deals with the effectiveness of pair check method in writing announcement text at class X SMA Swasta Raudhatul Hasanah Academic Year 2021/2022. Its objective is to find out whether the use pair check method has a significance effect in announcement text writing. Population and samples used in this study were all students of class X total 27 students. The method used in this study is an experimental method with a one group as pretest and posttest design .This study was carried on using quantitave method. The data was analysed by using t-test.The researcher found the pretest mean = 51,67 deviation standard = 3,67 and experienced an increase in the posttest mean = 81,85  deviation standard = 2,46. From this analysis, writer found that guided pair check method has significane in teaching writing announcement text. It can be proved by the result of the analysis showed that “ ” observed about 35,50 was higher than “ttable” about 2,056 with the level significance is 0,05% it implies that the teacher of English should use pair check method in teaching announcement text writing so that the teaching and learning process can be successful.
THE USE OF POP UP BOOK MEDIA IN WRITING DESCRIPTIVE TEXT AT VII GRADE IN SMP NEGERI 1 LAWE ALAS ACADEMIC YEAR 2022/2023
Authors: Ayu Anggraini Putri. S  , James Marudut  , Abdul Halim
Published: January 2024

This research deals with the use of pop up book media to improve students’ descriptive text writing class VII SMP Negeri 1 Lawe Alas Academic Year 2022/2023. Its objective is to find out whether the use of pop up book media has a significance effect in writing descriptive text. Population used in this research of class VII were 38 and all samples used in this research were 38 students. The method used in this research is an experimental method with a one group as pre-test and post-test design .This research was carried on using quantitave method. The data was analysed by using t-test. The researcher found the pre-test mean = 56,18 , deviation standard = 5,86 and experienced an increase in the pos-ttest mean = 79,34 deviation standard = 4,10. From this analysis, writer found that guided pop up book media has significance in teaching writing descriptive text. It can be proved by the result of the analysis showed that “ ” observed about 26,02 was higher than “t-table” about 2,026 with the level significance is 5% it implies that the teacher of English should use pop up book media in teaching writing descriptive text so that the teaching and learning process can be successful.
Las marcas de lo institucional en el funcionamiento de las redes de apoyo: el caso de docentes peruanos en tiempos de pandemia
Authors: Santos Anaya Martín , Duffó Nadia
Published: January 2024

Los docentes constituyen un pilar clave del proceso educativo. En una situación-límite disruptiva de la vida cotidiana, como aquella de la pandemia del COVID-19, ¿cómo fueron puestas en movimiento las redes personales de apoyo de los docentes para responder a sus emergentes necesidades laborales (enseñanza) y personales? ¿Cuál fue el papel de los marcos institucionales y organizacionales en este proceso? Este estudio busca responder estas preguntas a partir de material empírico de docentes peruanos de educación secundaria durante la pandemia del COVID-19. El diseño metodológico fue de carácter mixto (sociométrico y cualitativo). Se encontró una diversidad de procesos de movilización de los lazos de apoyo. En particular, destacamos la presencia de procesos de movilización mixtos. Asimismo, los marcos institucionales (familia) y organizacionales (instituciones educativas) permearon de diferentes maneras estos procesos. Los docentes recrearon rutinas organizacionales que existían antes de la pandemia, pero también desarrollaron nuevas prácticas de apoyo. Algunas de estas prácticas tendieron a rutinizarse, pero otras fueron resistidas, debido a sus consecuencias no buscadas negativas en las vidas de los docentes. Estos resultados aportan nuevos elementos analíticos a teorías existentes sobre procesos de movilización de lazos de apoyo.
Red de política contenciosa en Bolivia. Proyección de grafos bipartitos en datos de conflicto social de 2010 a 2020
Authors: Zeballos Puccherelli Maria Clara , Weise Gabriel
Published: January 2024

El conflicto social surge cuando uno o más grupos expresan reclamos hacia la autoridad y llevan a cabo diversas acciones coercitivas en tiempo y espacio para alcanzar sus objetivos. Nos preguntamos si los 10,000 conflictos registrados por la Fundación Unir Bolivia entre 2010 y 2020 revelan patrones de interacción y vínculos entre los grupos de protesta. Los datos basados en eventos tienen una naturaleza bidimensional que presentan desafíos analíticos, en este trabajo aplicamos la proyección de grafos bipartitos con el algoritmo "common neighbor", para obtener un grado unidimensional y aplicar el análisis de red. Como resultado, se obtuvo una red en la que 15 de cada 100 conflictos permiten al menos un vínculo entre dos de los más de 7,000 grupos de protesta, resultando en una estructura tipo núcleo-periferia que indica cierta segregación de los nodos con menor grado de vinculación. Adicionalmente, ponderamos la frecuencia de enlaces para eliminar grupos densos de conexión y confirmamos que la protesta individual y las alianzas momentáneas predominan en los conflictos. Los grupos con mayor grado de conexión están asociados con un menor costo de radicalidad y duración del conflicto y forman comunidades con roles específicos en la economía estatal.
PENETAPAN POTENSI UNGGULAN KECAMATAN DI KABUPATEN BANDUNG
Authors: Rukmana Asep Nana , Aviasti  , Amaranti Reni , Shakira Muhammad Akbar
Published: January 2024

Economic development is a continuous effort to improve people’s welfare. The main problem in regional development lies in the development of policies that are based on the specific characteristics of the area concerned (endogenous development) by using the potential of human resource, institutional, and natural resource locally (regions). Potential leading sectors in Bandung Regency are agriculture, plantation, livestock, fishery, industry, and tourism. Each sub-district in Bandung Regency region has potential leading sectors that need to be set as leading sectors to be further developed. The purpose of this research was to identify the variables that influence the determination of superior potential of sub-districts in Bandung Regency. The method used to create a model in determining the superior potential of sub-districts in Bandung Regency was the Analytical Hierarchy Process (AHP) method. In this research, 4 sub-districts were taken as an example of the model that were Majalaya, Banjaran, Soreang, and Cimenyan District. Leading sectors assessment of the four districts had the following priority sequence: Majalaya District is for the Industrial sector with a priority weight of 0.326, Agriculture 0.254, Tourism 0.163, Plantation 0.149, and Livestock 0.107; Banjaran District, had the Plantation sector with priority weights 0.294, Industry 0.207, Tourism 0.205, Agriculture 0.165, and Livestock 0.128; Soreang District, had the Agriculture sector with priority weights 0.278, Industry 0.222, Plantation 0.208, Tourism 0.199, and Livestock 0.093; Cimenyan District, had the Tourism sector with priority weights 0.373, Agriculture 0.223, Industry 0.195, Plantation 0.129, and Livestock 0.081. The sub-criteria/factors that were priorities in determining the superio sectors of the district, the top 5 of the 12 sub-criterias/factors were capital, technology, business management, market availability, and prices.
KUALITAS BISKUIT MP-ASI DARI TEPUNG KOMPOSIT KIMPUL-KACANG TUNGGAK DAN TEPUNG SAGU SELAMA PENYIMPANAN
Authors: Puspitasari Diana , Rejeki Fungki Sri , Wedowati Endang Retno , Koesriwulandari  , Kadir Akmarawita
Published: January 2024

Kimpul can be used as various types of products to increase added value, one of that is biscuits. The advantages of biscuits include long-lasting, also easy to digest. Therefore biscuits can be made as complementary foods for ASI (MP-ASI). MP-ASI biscuits are commonly consumed by children aged 12-24 months. MP-ASI biscuits with the raw material of kimpul flour still need other ingredients to increase the protein content in order to meet the quality requirements, such as cowpea flour. To improve the texture to make it softer, it is used sago flour. Sago flour, which has a high starch content, also functions as an adhesive. This study aimed to determine the quality of the MP-ASI biscuit product of kimpul-cowpea composite flour and sago flour during storage. The research used a single factor RAK, namely the type of packaging (PE pouch, aluminum foil pouch, PE-aluminum foil pouch) and repeated three times. Observation during storage was done in every two weeks for six months. The parameters taken were physical characteristics, water content, Aw, FFA, TPC, and peroxide number. The results showed that during storage, there was a decrease in the quality of physical characteristics, increased water content, Aw, FFA, peroxide number, and TPC. Based on physical and chemical quality, the treatment that experienced the lowest quality decline was P1 (Aluminum Foil).
Pemanfaatan Tempurung Kelapa sebagai Katalis pada Proses Konversi Minyak Curah Menjadi Biodiesel
Authors: Yustia Wulandari Mirzayanti  , Erlinda Ningsih  , Zain Lillahulhaq  , Zuhdi Ma’sum  , Chevy Renova  , Yasin Wijaya
Published: January 2024

Vegetable oil is one of the alternative sources of raw material for fuel oil to produce biodiesel. The conversion reaction process requires the help of a catalyst to break down triglycerides into methyl esters. Therefore, this study aims to determine the performance of catalysts made from coconut shells to convert bulk oil into biodiesel. The catalyst performance is focused on biodiesel's yield parameters through the influence factor of catalyst weight, molar ratio, and %FAME. This research was conducted in three stages, namely (1) catalyst preparation, (2) application of catalyst performance through the conversion process of bulk oil into biodiesel, (3) washing of biodiesel. The first stage was catalyst preparation carried out by utilizing a coconut shell, which was first made into charcoal, then activated using H3PO4 then sulfonated with H2SO4. The second stage was the conversion process of bulk oil into biodiesel using coconut shell catalysts through a transesterification process using variations in the catalyst's weight to bulk oil (0.5; 1; 1.5; 2; and 2.5% wt/wt) and bulk oil molar:methanol ratio (1: 3; 1: 6; and 1: 9). The third stage was the process of washing biodiesel using HCl. Based on this study's results, the best catalyst weight was 2% wt/wt with a molar ratio of 1: 9 resulting in the highest yield of 89% and the FAME of 80.36%.
Pengaruh Variasi Konsentrasi NaOH sebagai Nutrisi pada Performansi Biogas Tipe Portabel
Authors: Syamsuri  , Yustia Wulandari Mirzayanti  , Wiwik Widyo Widjajanti  , Samuel Kristian Bani
Published: January 2024

Biogas is a gas fuel produced by anaerobic activity or fermentation of organic materials, including human and animal waste, domestic (household) waste, or anaerobic degradation of organic materials by anaerobic bacteria. In this study, biogas was used as fuel with the addition of NaOH variations to the modified gasoline generator set engine so that it could work to produce electricity. This experiment used variations of 2, 4, 6, 8, and 10% NaOH nutrition with variations in the electric load of 10, 15, 20, 25, 30, 35, 40, 45, and 50 watts. These purposed to determine the performance of electric power, torque, and system efficiency. From the test, it found that the greater load of the lamp, the smaller performance of the electric power produced. The results showed that the greatest electric power was the variation of 2% NaOH nutrition with a lamp load of 10 watts, namely 39.33 watts. In the efficiency testing, the greater electric load system resulted efficiency system tent to be stable. The best system efficiency with 2% NaOH nutrition was compared to other nutritional variations. In testing of torque, the greater electric load torque resulted also greater torque. The greatest torque with 2%, NaOH nutrition compared to other nutritional variations.
Pengukuran dan Penentuan Prioritas Perbaikan Kualitas Layanan Jasa Service Kendaraan dengan Metode Service Quality dan Analytical Hierarchy Process
Authors: Krisnadhi Hariyanto  , Astria Hindratmo
Published: January 2024

The service business in Indonesia is growing every year and competition is getting tougher. One of the service businesses that is always growing is motor vehicle repair service. So that the vehicle service company must make service improvements to be able to compete. For this reason, the company needs to know the service performance so that the company must know the advantages and disadvantages of the services provided to customers. The purpose of this study is to measure the performance of vehicle service quality and determine service improvement priorities in increasing customer satisfaction and loyalty. The method used in this research is service quality and Analytical Hierarchy Process (AHP). The results of the study were to obtain 21 service attributes obtained from interviews. Then from the results of processing with the service quality method, it turns out that there is a gap in service quality based on perceptions and expectations because it is negative. So all the existing service attributes need to be improved. Meanwhile, the results of determining priorities that need to be improved on the 21 service attributes using the AHP method show that the mechanical speed attribute in car service is the first priority because it has the largest weight, namely 0.107. Then the service attribute that gets the last priority to be repaired is the equipment and equipment attribute with the smallest weight of 0.02 because the current dealer already has a fairly complete vehicle service tool.
Produksi Bahan Bakar Minyak (BBM) dari Sampah Botol Plastik Bekas Air Minum dengan Metode Pirolisis
Authors: Dhaniswara Trisna Kumala , Dian Fahriani
Published: January 2024

Garbage is the biggest contributor to environmental damage, especially plastic waste that is not processed properly. One of the problems in the community is the management of plastic waste, which until now has not been controlled. It is estimated that each person consumes 170 plastic bags each year and that around 500 billion to one trillion plastic bags are used worldwide. As well as more than 17 billion plastic bags distributed free of charge by supermarkets around the world for each year. In fact, if all the plastic bags on earth were opened, they could cover the entire surface of the earth up to 11 times. Plastic is a material that is very familiar in human life and has been considered a staple for household or domestic needs so that the presence of plastic waste is increasing. The purpose of this research is to convert plastic waste bottles used for drinking water into fuel oil using the pyrolysis method. The reactor used is pyrolysis with a temperature of 200 °C, 250 °C, 300 °C, 350 °C, and 400 °C for 30, 60, and 90 minutes. After the fuel is obtained, it is taken for analysis of its heating value, flash point, ash content, moisture content, and composition analysis. The results of this research will help the processing of plastic waste in addition to used plastic bottles.
Pengolahan Limbah Cair Bekas Pencucian Ikan Menggunakan Scirpus grossus
Authors: Atik Widiyanti  , Laily Noer Hamidah
Published: January 2024

Sidoarjo is one of the largest fish-producing districts in East Java. The high productivity of fisheries is in line with the increasing amount of waste produced. Both solid waste and liquid waste. Generally, liquid waste comes from water used for washing fish, usually brownish, cloudy and smells fishy. In addition, the concentration of organic matter is very high in the waste. The purpose of this study was to determine the reduction in levels of Total Suspended Solid (TSS), Total Dissolved Solid (TDS) and Biocamical Oxigen Demand (BOD) in these wastes using Scirpus grossus. The independent variable used is the variation in the number of plants (without plants, 5 plants and 10 plants). The waste used comes from the Fish Market Depot Kab. Sidoarjo. The research begins with the acclimatization process, then the phytoremediation test is carried out. Based on laboratory analysis, it was found that the best decrease in TSS, TDS and BOD was obtained from the reactor with 10 plants.
Analisis Pelaksanaan Pembangunan TPA Kabupaten Banyumas Akibat Kelongsoran dan Perubahan Desain Perencanaan
Authors: Agus Bambang Siswanto  , Mukhamad Afif Salim  , Hari Setijo Pudjihardjo  , Novike Dian Utami
Published: January 2024

Project is something with constrains can affect the time or duration of the work, slope slides and building redesigns that have an impact on delays in project implementation. Delays that occur can result in the presence of less work and work items that cause work to experience delays and increase the total cost of implementing the project or it can be said that the project suffers a loss. In this case, good time and coast management needs, can be solved properly. This challenge of a project is main to achieve the goals project within the constraints which generally the scope, schedule and budget of the project. Many methods used in scheduling, for expected scheduling planning. This research to get a logical schedule by rescheduling in accordance that it is hoped no more delays. Analytic research need of data such as schedules and budgeting directly the required data to the relevant parts, evaluating the schedule, followed by a new schedules with Microsoft Excel, and finally perform cost calculations with a balanced budget system. The result of this research the rescheduling is 440 days with the total RAB after rescheduling is Rp. 41,286,988,000,-, equal to the total cost before rescheduling, because of the work added and less and carried out a balanced budget.
Penerapan Metode Quality Function Deployment dan Antropometri dalam Perancangan Desain Mesin Cacah Sampah Organik dan Non Organik
Authors: Navik Kholili  , Astria Hindratmo  , Alfi Nugroho
Published: January 2024

The Garbage is residual material that has been disposed of and is no longer used, so that waste is considered something disgusting by some people. Garbage has two types, namely organic waste and inorganic waste. Therefore, good waste management is needed so that every day waste does not accumulate and does not disturb the surrounding environment. The purpose of this research is to design a garbage chopper that is useful for chopping organic and inorganic waste to help the waste processing process so that there is no buildup every day. The design of this chopping machine is made multi-functional that can chop organic or inorganic types of waste, designed according to the wishes of consumers or users and made ergonomically. The method used in this research is the Quality Function Deployment (QFD) method and the user's body Anthropometry so that the dimensions of the machine are in accordance with the user which causes the design to be ergonomic. The results of the research are to produce a design of organic and inorganic waste chopping machines that have specifications such as machines using mild steel so that they are easy to move, durable, speed up the chopping process because there is a speed setting, automatic. In addition, the machine has ergonomic dimensions from the results of the machine user's body measurements using the anthropometric method.
L’accompagnement des transitions professionnelles : un monde en soi ?
Authors: Gonnet Aurélie
Published: January 2024

Par-delà sa pluralité interne (acteurs, pratiques, dispositifs, publics, terminologies professionnelles…), l’accompagnement des transitions professionnelles constitue un monde social et professionnel en soi, au sein duquel circulent un certain nombre de représentations, de principes d’action, de modes de fonctionnement, d’outils, de ressources et d’individus –professionnels ou non–, et dont les contours sont relativement perceptibles –bien qu’amovibles. C’est ainsi que ce monde, à mesure qu’il prend forme et s’étend, semble comme absorber dans son giron des domaines et enjeux autrefois davantage distincts: la santé, le travail social, la création d’entreprise ou encore la formation dans son ensemble (initiale, continue, professionnelle, universitaire). L’examen de la série d’expériences rencontrées par celles et ceux qui vivent et/ou accompagnent des transitions professionnelles tend effectivement à donner corps à cette hypothèse. Il invite alors à documenter finement ce monde afin de passer d’un monde en soi (existant de fait) à un monde pour soi, c’est-à-dire conscient de soi et donc susceptible de nourrir un regard critique et réflexif.
Sulfenamidas e sulfenimidas - o efeito da estrutura na vulcanização do SBR através da modelagem molecular
Authors: Da Costa Helson Moreira , Ramos Valéria Dutra
Published: January 2024

O efeito da estrutura de aceleradores do tipo sulfenamidas e sulfenimidas sobre a vulcanização do copolímero de estireno-butadieno (SBR) foi investigado neste manuscrito. Mediante a modelagem molecular de vinte e três espécies químicas, os dados experimentais sobre o tempo de pré-cura (ts2) e a taxa de cura de composições SBR contendo negro de fumo foram correlacionados com os parâmetros teóricos (área superficial, polarizabilidade, eletrofilicidade, lipossolubilidade, entre outros) das moléculas dos diferentes aceleradores. Embora não possa ser descartado o efeito da carga de negro de fumo durante a cura, as equações de regressão obtidas foram satisfatórias e permitiram avaliar como diferentes estruturas moleculares podem tornar a vulcanização mais lenta ou mais rápida, além de interferir ou não em uma cura mais prematura.
Terapia nutricional enteral domiciliar
Authors: Da Costa Débora Eugênio , Vianna Lorena Guntendorfer , Marques Alex Pereira , De Lima Keiliane Cirilo , Fonseca Amanda Pinto , Ribeiro Monalysa Andrezza dos Santos , Gonzaga Izabela Ribeiro , Pereira Araída Dias
Published: January 2024

Introdução: A atenção domiciliar é um tipo de assistência voltada para a melhoria do bem-estar, recuperação ou cuidados clínico-nutricionais em seu próprio lar. Nesse contexto, uma das atividades essenciais é a terapia nutricional enteral domiciliar (TNED) que envolve a disponibilização de nutrientes utilizando a Nutrição Enteral (NE) em ambiente residencial. Objetivo: Descrever a assistência nutricional domiciliar na nutrição enteral.  Método: Estudo de pesquisa bibliográfica, do tipo revisão integrativa de literatura que percorreu o rigor metodológico de 6 etapas. Avaliou-se os estudos que responderam a pergunta norteadora: “Como é realizada a assistência nutricional domiciliar na NE?” e que atenderam os critérios de inclusão e exclusão. Resultados: Foram avaliados 14 artigos, com a predominância do tipo transversal, observacional e qualitativo (21,4%). Quanto ao idioma: 3 em português e 11 em inglês. A respeito do tema principal, 9 artigos abordavam a TNED, 3 sobre os cuidadores na TNED, 1 sobre TNED em idosos e 1 sobre TNED no público infanto-juvenil. Conclusão: As fórmulas de NE mais utilizadas em domicílio foram as dietas industrializadas e a via de acesso a nasoentérica.
Molecular characterization of whitefly on different host plants
Authors: Molina Rúbia de Oliveira , Hata Fernando Teruhiko , Walz Daniel Mazzieri
Published: January 2024

The objective of this work was to determine the whitefly (Bemisia tabaci Genn.) biotypes that occur in the state of Paraná. For the determination of the biotypes, whiteflies were collected in different host plants during 2016 in 21 cities of the state of Paraná, and molecular biology techniques were used for the analysis of the samples. Total insect DNA extraction was performed with Chelex® 100 resin, followed by PCR amplification of the mitochondrial cytochrome oxidase 1 (mtCOI) gene region. The PCR products were sequenced and analyzed with Mega 7.0.21 software along with the ClustalW2 site. The dendrogram of the samples generated along with the patterns of the other species showed a clustering of all the samples with those of the MEAM1 species (biotype B), being the only species found in the studied regions. The identity of the nucleotides among the whiteflies ranged from 96.94% to 100%. Biotype A was not found in regions where it was previously occurring, probably due to the MEAM 1 species. The whiteflies of the MEAM 1 species (biotype B) were found in 14 different plant species. These results are important in the current scenario of Paraná and could contribute to better efficiency in the management of this pest.
Prospecção científica e tecnológica de leveduras ambientais quanto à produção de enzimas hidrolíticas de interesse biotecnológico
Authors: Freire Antonia Fábia do Nascimento , Canto Eveleise Samira Martins , De Paiva Maykon Jhuly Martins , Dos Santos Taides Tavares
Published: January 2024

Leveduras são integrantes microbianos da biodiversidade brasileira que representam uma valiosa fonte de potenciais aplicações biotecnológicas. Esses micro-organismos que podem crescer e sobreviver em diferentes substratos naturais e produzir uma grande variedade de enzimas hidrolíticas, as quais podem ser empregadas no desenvolvimento de produtos e otimização de bioprocessos industriais. Neste estudo, foi realizada uma prospecção científica e tecnológica, do período entre 2012 e 2022, sobre emprego de enzimas hidrolíticas de leveduras ambientais. Para a prospecção científica, foram verificadas as produções sobre o tema nas bases MEDLINE/PubMed, Scientific Electronic Library Online (SCIELO) e Catálogo de teses dissertações da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Para a prospecção técnológica, foi realizada busca por depósito de patentes nos bancos de dados do Instituto Nacional da Propriedade Industrial (INPI), European Patent Office (EPO) e The United States Patent and Trademark Office (USPTO). Foram identificados um total de 3.067 artigos científicos, 143 dissertações ou teses e 267.352 depósitos de patentes relacionados às enzimas de interesse deste estudo (amilase, celulase, pectinase e protease), sendo o maior volume, tanto de produções científicas como tecnológicas, corresponte à enzima protease. Quanto à classificação internacional de patentes (IPC), houve destaque para a produção de álcool combustível, com quatro publicações no total. Concluiu-se, por meio deste estudo, que há diversas perspectivas de aplicações tecnológicas e de inovações envolvendo enzimas hidrolíticas advindas de leveduras e, os bancos de dados de publicações e de patentes podem servir como fonte de insights para inovações biotecnológicas envolvendo enzimas microbianas.
Estrutura e composição viral: Minecraft Education Edition como estratégia metodológica para estimular a colaboração e negociação de significados
Authors: Mulati Janaina Ciboto , Corazza Maria Júlia
Published: January 2024

O Projeto Interdisciplinar de Ciências, desenvolvido em um colégio da rede privada de um município do Estado do Paraná/Brasil, envolvendo alunos dos anos finais do Ensino Fundamental, serviu de espaço e contexto interativo para a realização de uma pesquisa, estruturada na abordagem qualitativa e pesquisa-ação. Como parte dessa pesquisa, este estudo objetivou identificar a colaboração e negociação de significados sobre a estrutura e composição química viral, durante atividades interativas, mobilizadas pela Aprendizagem Baseada em Projetos (ABP) e construção de modelos virais na Minecraft Education Edition. Esta investigação foi realizada com quinze dos alunos, utilizando um questionário, pré-teste e pós-teste, para a composição dos dados. O questionário buscou evidenciar os conhecimentos dos alunos acerca dos vírus e suas percepções quanto ao uso do Minecraft. A análise dos dados, fundamentada no método pragmático, revelou uma diversidade de concepções iniciais sobre os vírus, desde definições simplificadas até conceituações mais elaboradas. A colaboração e negociação de significados, propiciadas pelas interações dialógicas e elaboração coletiva de atividades, foram evidenciadas nas respostas pós-teste, caracterizadas por uma evolução nas compreensões acerca da relação entre a organização dos seres vivos e a classificação dos vírus como vivos ou não. Na análise dos dados referentes à utilização da Minecraft Education Edition em atividades escolares, foram identificadas experiências variadas, incluindo aspectos positivos e desafios. Concluímos que os espaços e contextos interativos, viabilizados pela ABP, aliada ao uso de jogos educacionais, podem estimular a colaboração e negociação de significados, potencializando a aprendizagem.
Tek Proseste Kompaund Elde Edebilen Tandem Bir Sistemle Yapılan Termoplastik Geri Dönüşümünün Çevreye ve Dünyaya Faydaları
Authors: Bakırcı Altuğ , Koçak Cem , Yamaç Özgür , Çakır Mustafa Cemal
Published: January 2024

The manufacture of polymers has experienced significant growth since the 1900s due to their favorable attributes such as ease of manufacturing, cost-effectiveness, desirable chemical and physical qualities, as well as their lightweight nature. The escalating utilization of plastic has begun to inflict damage upon the environment, organisms, and human well-being, hence giving rise to challenges pertaining to recycling and waste management. In the process of recycling, it is necessary to subject thermoplastics to two cycles of melting in order to get the correct material characteristics. As a result of the restricted number of mechanical recycling cycles for plastics, thermoplastics have an accelerated rate of expiration, double the usual timeframe. In the proposed tandem recycling process, a single melting operation can subject the trash that has already undergone recycling to a second round of recycling. By using this approach, the potential for enhancing the reusability of thermoplastic waste is heightened. The absence of an additional heating process results in energy savings, leading to a notable reduction in expenses.
PARACOCCIDIOIDOMICOSE TESTICULAR: UM DIAGNÓSTICO DIFERENCIAL DE CÂNCER DE TESTÍCULO
Authors: Scaloppe Tane Machado , Passos Ana Rita de Oliveira , Lemes Polyana Silva , Santos Ygor Laurindo Albues , Zuntini Gabriel Nogueira , De Lima Vanessa Evelyn Nonato , Buzatto Maria Luisa , Costa Hudson Marcelo Silva Gonçalves
Published: January 2024

A paracoccidioidomicose é uma doença endêmica sistêmica causada por fungos, com o maior número de casos em estados do centro-oeste e norte do brasil, em regiões com atividades agrícolas por estar relacionado ao manejo do solo contaminado com o fungo. A doença ocorre predominantemente no sexo masculino, em adulto de 30-50 anos por reativação de foco endógeno latente, podendo estar associada frequentemente ao tabagismo e não há uma relação frequente com doenças imunossupressoras. O maior número de casos acontece no pulmão devido a inalação do patógeno, além de linfonodos e forma cutânea, sendo que em outras localidades apresentam uma forma rara da doença.
EDUCAÇÃO EM SAÚDE COMO ESTRATÉGIA PARA O COMBATE DA POLIOMIELITE: RELATO DE EXPERIÊNCIA
Authors: De Souza Luana Jessica Ferreira , De Oliveira Natan Duarte , Da Silva Patricia Fernandes , Bezerra Renan Pinheiro , Felix Selma Teixeira , Servini Kayan Oliveira , Chrizostimo Miriam Marinho , Ortiz Sánchez Maritza Consuelo , Nassar Pedro Ruiz Barbosa , Braga André Luiz de Souza
Published: January 2024

A poliomielite é uma doença infectocontagiosa viral aguda, caracterizada por um quadro de paralisia flácida, de início súbito, que ocorre em cerca de 1% das infecções causadas pelo poliovírus. Acomete em geral os membros inferiores, de forma assimétrica, tendo como principais características a flacidez muscular, com sensibilidade preservada, e a arreflexia no segmento atingido. (BRASIL, 2017). Objetivo: Relatar a experiência dos acadêmicos de enfermagem do sexto período na realização de ações de educação em saúde como ferramenta na assistência em saúde, como prevenção da paralisia infantil. Método: No ano de 2023 foi realizada a ação de educação em saúde sobre poliomielite, em uma Policlínica vinculada ao Sistema único de Saúde, num município da Região da Metropolitana II do Estado do Rio de Janeiro. Desta ação emergiu o presente relato de experiência dos acadêmicos de enfermagem do sexto período, da Escola de Enfermagem Aurora de Afonso Costa, da Universidade Federal Fluminense. As estratégias utilizadas nesta educação em saúde foram o flyer e o diálogo entre os estudantes e os usuários que aguardavam o atendimento na Policlínica. Resultados e discussão: Percebe-se que a educação em saúde teve resultado satisfatório quanto a proposta, visto que a interação entre os participantes foi intensa, pois a dúvida da população que frequentava a policlínica sobre a poliomielite foi esclarecida. Observou-se com relação a caderneta de vacinação havia dúvidas sobre a importância da vacina para a prevenção do agravo à saúde do indivíduo e da comunidade. Conclusão: Nota-se que houve aprendizagem quanto a doença poliomielite para os acadêmicos e para a população. A experiência foi exitosa, pois os usuários ficaram atentos à explicação dos acadêmicos e posteriormente responderam as perguntas disparadoras sobre a temática. Atenta-se que, por meio da comunicação com pequenos grupos, geralmente é o modo adequado de troca e saneamento de dúvidas apresentadas. Compreende-se que a ampliação das informações dadas de forma precisa, eficiente, eficaz e acessível é fundamental para a conscientização da população sobre os riscos, os métodos de prevenção e a importância da vacinação, para a redução do número de casos de poliomielite no país, por considerar que o Brasil já foi modelo.
BNCC, RACIONALIDADE NEOLIBERAL E O ENSINO DE FILOSOFIA
Authors: Lima Galba Vanderlei Batista
Published: January 2024

O tema deste artigo é a Base Nacional Comum Curricular, BNCC, e suas implicações para o ensino de Filosofia. Nele, pretendo fazer uma leitura da BNCC a partir de uma racionalidade neoliberal, identificando as consequências para o ensino de Filosofia. Inicialmente apresento a BNCC como um exemplo de gestão não democrática, depois procuro estabelecer uma relação entre o conceito de competência preconizado na BNCC e o conceito de governamentalidade no pensamento de Foucault, e por fim, irei abordar os desafios que se apresentam para o ensino de Filosofia a partir da BNCC. O pressuposto é de que uma política educacional voltada para a qualificação de mão-de-obra para o trabalho não tem interesse no desenvolvimento do pensamento crítico. Nesse cenário, a Filosofia é desvalorizada e sem um futuro certo como disciplina escolar.
CORPO E PERFORMANCE NA ATUAÇÃO DO TRADUTOR/INTÉRPRETE DE LIBRAS EM MÍDIAS DIGITAIS YOUTUBE, GOOGLE MEET E INSTAGRAM
Authors: Da Silva Amauri Moret , Aguiar João Vitor Lemos , Pegoraro Odirlei , Mota Vinicius Alan Maçal
Published: January 2024

A pesquisa tem por objetivo geral compreender a atuação do profissional tradutor/intérprete de Libras em mídias digitais (YouTube, Google Meet e Instagram) considerando suas vivências e práticas na tradução/interpretação português/Libras, como também Libras/português, cujo principal campo de atuação ocorre no canal do Youtube em lives musicais. Parte-se da concepção que a atuação do tradutor/intérprete de Libras nos meios digitais proporciona a acessibilidade linguística à comunidade surda. Desse modo, o corpo e performance do tradutor/intérprete de Libras são fundamentais na transliteração das línguas envolvidas no processo comunicativo. A metodologia adotada é de cunho qualitativo, observação participante e revisão bibliográfica. Selecionou-se o corpus por meio dos ambientes virtuais em que versa a atuação do tradutor/intérprete de Libras, desse modo observou-se o corpo e a performance do tradutor/intérprete de Libras nas traduções musicais. O texto segue uma linha cronológica visando expor primeiramente a contextualização das mídias digitais em ascensão, bem como os meios em que os profissionais mais atuam. Com este estudo alcançou-se aos seguintes resultados; há diferenças entre os tradutores/intérpretes analisados, de um lado os profissionais fazem escolhas linguísticas inadequadas durante a live, já do outro lado é assertivo nos elementos de concordância corporal.
TÉCNICA INOVADORA NO TRATAMENTO DO GLAUCOMA: CICLOPLASTIA POR ULTRASSOM
Authors: Martins Vitor José Gonçalves , Vasconcellos Marcus , Ferreira João Maria
Published: January 2024

Introdução: O glaucoma é uma das principais causas de cegueira irreversível, afetando mais de 60 milhões de pessoas em todo o mundo. A crescente prevalência de uma doença crônica constitui uma carga significativa para os sistemas de saúde, juntamente com o custo de vida associado a visitas de controle, testes e gerenciamento de uma população em envelhecimento. Nesse contexto, uma nova tecnologia, a cicloplastia por ultrassom (UCP) foi desenvolvida, e cada vez mais, utilizada na prática. Objetivo: O presente relatório de pesquisa tem como finalidade, por meio de uma revisão literária sobre o assunto, relatar um pouco mais sobre a cicloplastia por ultrassom, vantagens, desvantagens, resultados e prognósticos, visto que é um método novo e pouco conhecido, mas que vem ganhando muita importância no cenário oftalmológico mundial. Métodos: Foram utilizados 18 artigos para realização deste trabalho, e todos relatam sobre a cicloplastia ultrassônica, trazendo informações precisas e confiáveis através de relatos de caso, revisão literária e estudos prospectivos. Resultados: Diversos artigos analisados mostraram que a UCP é um método para tratamento do glaucoma com nível considerável de eficácia, podendo ser repetida várias vezes para melhor sucesso terapêutico. Logo esse método deve ser considerado no tratamento de glaucoma, visto que tem alta resolubilidade aliada a baixos efeitos colaterais graves comparados a outros métodos.
Prokaryotic expression and characterisation of recombinant M1 protein of an Indian H5N1 avian influenza virus
Authors: SYED ZOHRA , VENKATESH GOVINDARAJULU , MURUGKAR HARSHAD V , BEDEKAR MEGHA KADAM , NAGARAJAN SHANMUGASUNDARAM , DUBEY SHIV CHANDRA
Published: January 2024

The type specific Matrix 1 (M1) protein of an Indian H5N1 avian influenza virus (AIV) was expressed as a histidine- tagged fusion protein in a prokaryotic expression system and characterized. The M1 gene was amplified by reverse transcription PCR using appropriately designed primers and cloned into the expression vector, pET28a (+). The orientation and reading frame of the recombinant expression construct (pET-M1) was confirmed by sequence analysis. The derived amino acid sequence homology between M1 of AIV H5N1 and other reference AIV subtypes was found to be 93.7% to 99.2%. The 33kDA recombinant M1 (rM1) protein was expressed as inclusion body after induction with 1 mM IPTG in E. coli BL21 (DE3)pLysS cells. The protein was purified to near homogeneity by affinity chromatography using Ni- NTA agarose column. The yield of the purified rM1 was found to be 2 mg/100 ml of induced culture. The rM1 was found to react specifically with H1-H15 AIV subtype specific sera in Western blot. The results indicated that the purified rM1 protein could be used as antigen for detection of type specific AIV antibodies by immunoassays.
Changes in phosphatases activities in uterine secretion during estrous cycle:A possible indicator of important biochemical changes in buffaloes uterine microenvironment
Authors: GHOSH JYOTIRMOY , ROY SUDHIR C , SUGANTHI R UMAYA
Published: January 2024

Defining biochemical nature of uterine secretion has been the focus of recent research interest due to conception failure after embryo transfer and normal pregnancy in ruminants including buffaloes. Proteins are important as biomolecules for cell function, and phosphatases control the protein function. Therefore identification of changes on secretory proteins and phosphatases activities during early luteal, mid luteal, late luteal and follicular stages of estrous cycle in buffaloes is attempted. The luteal stages uterine secretion contained significantly more protein as compared to the follicular stages of cycle. Two-fold increase in specific and total activities of acid phosphatases during mid-luteal phase and 10-fold specific and 100-fold increase in total alkaline phosphatases activities during late luteal and follicular stages was observed as compared to other stages of the cycle. Corpus luteum growth and regression did not influence the total uterine protein secretion or phosphatases activities. The changes of phosphatases in buffalo uterine secretion during different stages of estrous cycle might be the indicator of the process of endometrial epithelial differentiation and degeneration.
In vitro evaluation of antioxidant properties of Chlorella vulgaris and its derivatives for use as antioxidant supplements in animal production
Authors: SIKIRU A B , ARANGASAMY A , ALEMEDE I C , EGENA S S A , BHATTA R , RAO S B N
Published: January 2024

Bioactive compounds with varied functional properties found in microalgae such as Chlorella vulgaris can serve as antioxidants supplement against free radicals causing oxidative stress in food animals, but there is a need to quantify these antioxidants in the algae for effective applications. Therefore, this study quantified antioxidants in Chlorella vulgaris and derivatives prepared with the microalgae biomass for enhancing its application in animal nutrition. Eight derivatives prepared using the Chlorella vulgaris biomass were subjected to in vitro antioxidant supplements in animals for attenuation of oxidative stress to improve performances and productivity because of their antioxidant potentials. Antioxidant assessments including ferric antioxidant power, hydrogen peroxide scavenging potential, total antioxidant capacity, and total phenolic content. The results showed that the mean total capacities of the preparations were 621.83±36.41 µmol/g ascorbic acid equivalent, ferric reducing antioxidant power (FRAP) was 1.28±0.09, while the hydrogen peroxide scavenging (HPS) potential was 34.04±3.18%, and the total phenolic content was 30.54 mg/g ellagic acid equivalent. It was concluded from these observations that the microalgae Chlorella vulgaris and its derivatives as prepared in this study could be used as antioxidant supplements in animals for attenuation of oxidative stress to improve performances and productivity because of their antioxidant potentials.
Differential detection and molecular characterization of multiple avian oncogenic viruses in backyard poultry of Tamil Nadu, India
Authors: PRABHU M , MALMARUGAN S , ANNE N SWEETLINE , GOPAL S , GOPAL K , KUMAR V , RAJESWAR J JOHNSON
Published: January 2024

The avian oncogenic viruses include Mareks disease virus (MDV), Avian Leukosis virus (ALV) and Reticuloendotheliosis virus (REV). All the three viruses as etiological agents of single and/or multiple oncogenic infections were reported previously in broiler and layer chickens. The present study describes the detection of these multiple oncogenic viruses by multiplex PCR from suspected samples collected from the native chicken of Tamil Nadu, a southern state of India. Out of 51 samples tested, 10 were found positive for MDV and 25 positive for ALV subgroup E and 6 samples positive for REV. Further, the representative samples were subjected to sequencing and phylogenetic analysis. The molecular characterisation of the present MDV strains showed the close proximity with strains from Tamil Nadu, Punjab, and Kerala states of India with bootstrap value of 97%. Further, the current REV strain showed close relatedness with the previously known Tamil Nadu REV isolate with bootstrap value of 66%. The ALV strain identified from the backyard poultry was found to be endogenous virus and showed close identity with ALV-E China isolate with bootstrap value of 78%.