bluuebunny/crossref_metadata_2025 huggingface dataset:
Surface Electromyography Thresholds as a Measure for Performance Fatigability During Incremental Cycling in Patients With Neuromuscular Disorders
Authors: Voet Nicoline B. M. , Saris Christiaan G. J. , Thijssen Dick H. J. , Bastiaans Vincent , Sluijs David E. , Janssen Mariska M. H. P.
Published: March 2022

In healthy persons, there is an excellent relation between the timing of the (two) surface electromyography (sEMG) thresholds and the (two) ventilatory thresholds during exercise. The primary aim of this study was to determine the relative timing of both sEMG and ventilatory thresholds in patients with neuromuscular disorders compared with healthy subjects during a maximal ergospirometry cycling test. We hypothesized that in patients with neuromuscular disorders, the sEMG thresholds would occur relatively earlier in time than the ventilatory thresholds, compared to healthy subjects, because performance fatigability occurs more rapidly. In total, 24 healthy controls and 32 patients with a neuromuscular disorder performed a cardiopulmonary exercise test on a bicycle using a 10-min ramp protocol, during which we collected ergospirometry data: power at both ventilatory and sEMG thresholds, and sEMG data of lower leg muscles. In line with our hypothesis, normalized values for all thresholds were lower for patients than healthy subjects. These differences were significant for the first ventilatory (p = 0.008) and sEMG threshold (p < 0.001) but not for the second sEMG (p = 0.053) and ventilatory threshold (p = 0.238). Most parameters for test–retest reliability of all thresholds did not show any fixed bias, except for the second ventilatory threshold. The feasibility of the sEMG thresholds was lower than the ventilatory thresholds, particularly of the first sEMG threshold. As expected, the sEMG thresholds, particularly the first threshold, occurred relatively earlier in time than the ventilatory thresholds in patients compared with healthy subjects. A possible explanation could be (a combination of) a difference in fiber type composition, disuse, and limited muscle-specific force in patients with neuromuscular disorders. sEMG measurements during submaximal dynamic exercises are needed to generalize the measurements to daily life activities for future use in prescribing and evaluating rehabilitation interventions.
Acute and Chronic Bone Marker and Endocrine Responses to Resistance Exercise With and Without Blood Flow Restriction in Young Men
Authors: Bemben Debra A. , Sherk Vanessa D. , Buchanan Samuel R. , Kim SoJung , Sherk Kyle , Bemben Michael G.
Published: March 2022

In this study, we compared acute and chronic bone marker and hormone responses to 6 weeks of low intensity (20% 1RM) blood flow restriction (BFR20) resistance training to high intensity (70% 1RM) traditional resistance training (TR70) and moderate intensity (45% 1RM) traditional resistance training (TR45) in young men (18–35 years). Participants were randomized to one of the training groups or to a control group (CON). The following training programs were performed 3 days per week for 6 weeks for knee extension and knee flexion exercises: BFR20, 20%1RM, 4 sets (30, 15, 15, 15 reps) wearing blood flow restriction cuffs around the proximal thighs; TR70, 70% 1RM 3 sets 10 reps; and TR45, 45% 1RM 3 sets 15 reps. Muscle strength and thigh cross-sectional area were assessed at baseline, between week 3 and 6 of training. Acute bone marker (Bone ALP, CTX-I) and hormone (testosterone, IGF-1, IGFBP-3, cortisol) responses were assessed at weeks 1 and 6, with blood collection done in the morning after an overnight fast. The main findings were that the acute bone formation marker (Bone ALP) showed significant changes for TR70 and BFR20 but there was no difference between weeks 1 and 6. TR70 had acute increases in testosterone, IGF-1, and IGFBP-3 (weeks 1 and 6). BFR20 had significant acute increases in testosterone (weeks 1 and 6) and in IGF-1 at week 6, while TR45 had significant acute increases in testosterone (week 1), IGF-1 (week 6), and IGFBP-3 (week 6). Strength and muscle size gains were similar for the training groups. In conclusion, low intensity BFR resistance training was effective for stimulating acute bone formation marker and hormone responses, although TR70 showed the more consistent hormone responses than the other training groups.
Genome-Wide Analysis and Expression Profiles of Ethylene Signal Genes and Apetala2/Ethylene-Responsive Factors in Peanut (Arachis hypogaea L.)
Authors: Cui Yuanyuan , Bian Jianxin , Guan Yu , Xu Fangtao , Han Xue , Deng Xingwang , Liu Xiaoqin
Published: March 2022

Peanut is an important oil and economic crop widely cultivated in the world. It has special characteristics such as blooming on the ground but bearing fruits underground. During the peg penetrating into the ground, it is subjected to mechanical stress from the soil at the same time. It has been proved that mechanical stress affects plant growth and development by regulating the ethylene signaling-related genes. In this study, we identified some genes related to ethylene signal of peanut, including 10 ethylene sensors, two constitutive triple responses (CTRs), four ethylene insensitive 2 (EIN2s), four ethylene insensitive 3 (EIN3s), six EIN3-binding F-box proteins (EBFs), and 188 Apetala2/ethylene-responsive factors (AP2/ERFs). One hundred and eighty-eight AP2/ERFs were further divided into four subfamilies, 123 ERFs, 56 AP2s, 6 Related to ABI3/VP1 (RAVs), and three Soloists, of them one hundred and seventy AP2/ERF gene pairs were clustered into segmental duplication events in genome of Arachis hypogaea. A total of 134, 138, 97, and 150 AhAP2/ERF genes formed 210, 195, 166, and 525 orthologous gene pairs with Arachis duranensis, Arachis ipaensis, Arabidopsis thaliana, and Glycine max, respectively. Our transcriptome results showed that two EIN3s (Arahy.J729H0 and Arahy.S7XF8N) and one EBFs (Arahy.G4JMEM) were highly expressed when mechanical stress increased. Among the 188 AhAP2/ERF genes, there were 31 genes with the fragments per kilobase of exon model per million mapped fragments (FPKM) ≥ 100 at least one of the 15 samples of Tifrunner. Among them, three AhAP2/ERFs (Arahy.15RATX, Arahy.FAI7YU, and Arahy.452FBF) were specifically expressed in seeds and five AhAP2/ERFs (Arahy.HGAZ7D, Arahy.ZW7540, Arahy.4XS3FZ, Arahy.QGFJ76, and Arahy.AS0C7C) were highly expressed in the tissues, which responded mechanical stress, suggesting that they might sense mechanical stress. Mechanical stress simulation experiment showed that three AhAP2/ERFs (Arahy.QGFJ76, Arahy.AS0C7C, and Arahy.HGAZ7D) were sensitive to mechanical stress changes and they all had the conservative repressor motif (DLNXXP) in the C-terminus, indicated that they might transmit mechanical stress signals through transcriptional inhibition. This study reveals the regulatory landscape of ethylene signal-related genes in peanut, providing valuable information for the mining of target genes for further study.
Comparative Gene Expression and Physiological Analyses Reveal Molecular Mechanisms in Wound-Induced Spore Formation in the Edible Seaweed Nori
Authors: Guan Xiaowei , Mao Yunxiang , Stiller John W. , Shu Shanshan , Pang Ying , Qu Weihua , Zhang Zehao , Tang Fugeng , Qian Huijuan , Chen Rui , Sun Bin , Guoying Du , Mo Zhaolan , Kong Fanna , Tang Xianghai , Wang Dongmei
Published: March 2022

Genetic reprogramming of differentiated cells is studied broadly in multicellular Viridiplantae as an adaptation to herbivory or damage; however, mechanisms underlying cell development and redifferentiation are largely unknown in red algae, their nearest multicellular relatives. Here we investgate cell reprogramming in the widely cultivated, edible seaweed Neopyropia yezoesis (“nori”), where vegetative cells in wounded blades differentiate and release as large numbers of asexual spores. Based upon physiological changes and transcriptomic dynamics after wound stress in N. yezoensis and its congener Neoporphyra haitanensis, another cultivar that does not differentiate spores after wounding, we propose a three-phase model of wound-induced spore development in N. yezoensis. In Phase I, propagation of ROS by RBOH and SOD elicites systematic transduction of the wound signal, while Ca2+ dependent signaling induces cell reprogramming. In Phase II, a TOR signaling pathway and regulation of cyclin and CDK genes result in cell divisions that spread inward from the wound edge. Once sporangia form, Phase III involves expression of proteins required for spore maturation and cell wall softening. Our analyses not only provide the first model for core molecular processes controlling cellular reprogramming in rhodophytes, but also have practical implications for achieving greater control over seeding in commercial nori farming.
Improved Reference Genome Annotation of Brassica rapa by Pacific Biosciences RNA Sequencing
Authors: Zhang Zhicheng , Guo Jing , Cai Xu , Li Yufang , Xi Xi , Lin Runmao , Liang Jianli , Wang Xiaowu , Wu Jian
Published: March 2022

The species Brassica rapa includes several important vegetable crops. The draft reference genome of B. rapa ssp. pekinensis was completed in 2011, and it has since been updated twice. The pangenome with structural variations of 18 B. rapa accessions was published in 2021. Although extensive genomic analysis has been conducted on B. rapa, a comprehensive genome annotation including gene structure, alternative splicing (AS) events, and non-coding genes is still lacking. Therefore, we used the Pacific Biosciences (PacBio) single-molecular long-read technology to improve gene models and produced the annotated genome version 3.5. In total, we obtained 753,041 full-length non-chimeric (FLNC) reads and collapsed these into 92,810 non-redundant consensus isoforms, capturing 48% of the genes annotated in the B. rapa reference genome annotation v3.1. Based on the isoform data, we identified 830 novel protein-coding genes that were missed in previous genome annotations, defined the untranslated regions (UTRs) of 20,340 annotated genes and corrected 886 wrongly spliced genes. We also identified 28,564 AS events and 1,480 long non-coding RNAs (lncRNAs). We produced a relatively complete and high-quality reference transcriptome for B. rapa that can facilitate further functional genomic research.
How Does Crop Rotation Influence Soil Moisture, Mineral Nitrogen, and Nitrogen Use Efficiency?
Authors: Yang Rui , Liu Ke , Harrison Matthew Tom , Fahad Shah , Wang Zhuangzhi , Zhou Meixue , Wang Xiaoyan
Published: March 2022

Rice–wheat (RW) cropping systems are integral to global food security. Despite being practiced for decades, Chinese RW cropping systems often suffer from low productivity and poor nitrogen use efficiency (NUE), reflecting management approaches that are not well-contextualized to region and season. Here, we develop the best management guides for N fertilizer in RW systems that are designed to help raise the productivity, NUE, and environmental sustainability of winter wheat over the long term. 2-year field experiments were conducted with four N fertilizer rates (0, 135, 180, and 225 kg N ha–1), allowing contrasts of yields, soil moisture, and NUE of wheat in RW in the humid climates zones on the Jianghan Plain. We compared RW systems with soybean/maize dryland wheat (DW) systems that are similarly endemic to China: after soybean/maize is harvested, soils are often drier compared with moisture content following rice harvest. With high seasonal N application rates (180–225 kg N ha–1), wheat crop yields increased by 24% in RW which were greater than comparable yields of wheat in DW, mainly due to greater kernels per spike in the former. Across treatments and years, N accumulation in plant tissue and kernel dry matter of DW was higher than that in RW, although mean agronomic efficiency of nitrogen (AEN) and physiological efficiency of nitrogen (PEN) of RW systems were greater. As N application rates increased from 135 to 225 kg ha–1, AEN and PEN of DW decreased but changed little for RW. Soil ammonium N was much lower than that of nitrate N; changes in NH4+ and NO3– as a consequence of increasing N fertilization were similar for RW and DW. We recommend that tactical application of N fertilizer continue seasonally until midgrain filling for both the DW and RW systems. At fertilization rates above 180 kg N ha–1, yield responses disappeared but nitrate leaching increased significantly, suggesting declining environmental sustainability above this N ceiling threshold. Collectively, this study elicits many functional and agronomic trade-offs between yields, NUE, and environmental sustainability as a function of N fertilization. Our results show that yield and NUE responses measured as part of crop rotations are both more robust and more variable when derived over multiple seasons, management conditions, and sites.
Positive Psychology: Looking Back and Looking Forward
Authors: Ryff Carol D.
Published: March 2022

Envisioning the future of positive psychology (PP) requires looking at its past. To that end, I first review prior critiques of PP to underscore that certain early problems have persisted over time. I then selectively examine recent research to illustrate progress in certain areas as well as draw attention to recurrent problems. Key among them is promulgation of poorly constructed measures of well-being and reliance on homogeneous, privileged research samples. Another concern is the commercialization of PP, which points to the need for greater oversight and quality control in profit-seeking endeavors. Looking ahead, I advocate for future science tied to contemporary challenges, particularly ever-widening inequality and the pandemic. These constitute intersecting catastrophes that need scientific attention. Such problems bring into focus “neglected negatives” that may be fueling current difficulties, including greed, indifference, and stupidity. Anger, which defies easy characterization as positive or negative, also warrants greater scientific study. Going forward I advocate for greater study of domains that likely nurture good lives and just societies – namely, participation in the arts and encounters with nature, both currently under study. Overall, my entreaty to PP is to reckon with persistent problems from its past, while striving toward a future that is societally relevant and virtuous.
How I Speak Defines What I Do: Effects of the Functional Language Proficiency of Host Country Employees on Their Unethical Pro-organizational Behavior
Authors: Shen Ya Xi , Zhang Chuang , Zuo Lamei , Zhou Xingxing , Deng Xuhui , Zhang Long
Published: March 2022

Functional language has been used in many multinational corporations (MNCs) as a way to overcome the problems caused by the coexistence of multiple languages in the workplace. The existing literature has explored the importance, adoption, and effectiveness of functional language. Yet, how functional language shapes host country employees’ moral cognition and behavior is insufficiently researched. Guided by the Social Identity Theory, this manuscript shows that host country employees’ functional language proficiency (i.e., English) enhances their unethical pro-organizational behavior through their linguistic group identification and moral disengagement. We tested our predictions using the data collected from 309 full-time host country employees through an online survey, and the results generally supported our hypotheses. The findings make contributions to both international management and language literature and organizational moral behavior literature.
Mediation-Moderation Links Between Mothers' ACEs, Mothers' and Children's Psychopathology Symptoms, and Maternal Mentalization During COVID-19
Authors: Dollberg Daphna G. , Hanetz-Gamliel Keren
Published: March 2022

Research has suggested adverse childhood experiences (ACEs) as a transdiagnostic risk factor for a variety of affective disorders. They are also linked with a parent's tendency toward affect dysregulation and hyperarousal, which may interfere with parenting and children's wellbeing. On the other hand, maternal mentalization can serve as a moderating factor that can help parents regulate their arousal, shielding children during adverse circumstances. We studied the mediated links between ACEs and mothers' and children's psychopathology symptoms during COVID-19 to determine whether maternal mentalization and the child's age moderate these links. Using results from 152 Israeli mothers of children aged 3–12 years recruited during the month-long lockdown in Israel, we documented that the mothers' ACEs were linked with increased risk of depressive and anxiety symptoms and with children's internalizing and externalizing behaviors. Moreover, as hypothesized, the mothers' symptoms of depression and anxiety mediated the links between their ACEs and their children's internalizing behaviors. In addition, the mothers' mentalization skills and, in the case of their depressive symptoms, their child's age, moderated these indirect links. For mothers of young children (3–6 years old) with higher mentalization levels, the link between the mothers' ACEs and the children's behavior problems was weaker compared to mothers with low mentalization levels. For mothers of older children (6–12 years old), and only in the case of maternal depressive symptoms, higher levels of maternal mentalization were linked with more internalizing behaviors. We discuss the potential clinical implications of the findings.
Effects of Online Learning on College Students in Eastern China: A Structural Equation Model
Authors: Zhu Junqi , Zhao Haixia , Wang Xue , Yang Li , Qin Zhiyuan , Geng Jichao
Published: March 2022

With the spread of COVID-19 worldwide, online education is rapidly catching on, even in some underdeveloped countries and regions. Based on Bandura's ternary learning theory and literature review, this paper takes online learning of college students as the research object and conducts an empirical survey on 6,000 college students in East China. Based on literature review and factor analysis and structural equation model, this paper discusses the relationship among learning cognition, learning behavior, and learning environment in online learning of college students. The learning behavior includes interactive communication, self-discipline mechanism, classroom learning, and study after class. The learning environment includes teaching ability, knowledge system, platform support, process control, and result evaluation; learning cognition includes learning motivation, information perception, and adaptability. It is found that the learning environment has a significant positive impact on learning behavior, and learning cognition has a significant positive impact on learning behavior. It is uncertain whether the learning environment significantly impacts learning cognition. At the learning environment level, the teaching ability (0.59) has the most significant impact on the learning environment, followed by result evaluation (0.42), platform support (0.40), process control (0.33), and knowledge system (0.13). In terms of the influence on learning behavior, classroom learning has the most significant impact (0.79), followed by self-discipline mechanism (0.65), study after class (0.54), and interactive communication (0.44). In terms of learning cognition, information perception (0.62) has the most significant influence, followed by learning motivation (0.50) and adaptability (0.41). This paper suggests strengthening the construction of platforms and digital resources to create a more competitive learning environment. Improve process management and personalized online services, constantly stimulate students' enthusiasm for independent online learning, and cultivate students' online independent learning ability to promote the sustainable and healthy development of online education.
Critical Analysis of Deconfounded Pretraining to Improve Visio-Linguistic Models
Authors: Cornille Nathan , Laenen Katrien , Moens Marie-Francine
Published: March 2022

An important problem with many current visio-linguistic models is that they often depend on spurious correlations. A typical example of a spurious correlation between two variables is one that is due to a third variable causing both (a “confounder”). Recent work has addressed this by adjusting for spurious correlations using a technique of deconfounding with automatically found confounders. We will refer to this technique as AutoDeconfounding. This article dives more deeply into AutoDeconfounding, and surfaces a number of issues of the original technique. First, we evaluate whether its implementation is actually equivalent to deconfounding. We provide an explicit explanation of the relation between AutoDeconfounding and the underlying causal model on which it implicitly operates, and show that additional assumptions are needed before the implementation of AutoDeconfounding can be equated to correct deconfounding. Inspired by this result, we perform ablation studies to verify to what extent the improvement on downstream visio-linguistic tasks reported by the works that implement AutoDeconfounding is due to AutoDeconfounding, and to what extent it is specifically due to the deconfounding aspect of AutoDeconfounding. We evaluate AutoDeconfounding in a way that isolates its effect, and no longer see the same improvement. We also show that tweaking AutoDeconfounding to be less related to deconfounding does not negatively affect performance on downstream visio-linguistic tasks. Furthermore, we create a human-labeled ground truth causality dataset for objects in a scene to empirically verify whether and how well confounders are found. We show that some models do indeed find more confounders than a random baseline, but also that finding more confounders is not correlated with performing better on downstream visio-linguistic tasks. Finally, we summarize the current limitations of AutoDeconfounding to solve the issue of spurious correlations and provide directions for the design of novel AutoDeconfounding methods that are aimed at overcoming these limitations.
A Computational Method for Modeling Spatiotemporal Variability of Hydrodynamic Properties in Sandy Soil Under Drainage and Recharge
Authors: Gumiere Silvio J. , Periard Yann , Celicourt Paul , Gumiere Thiago , Lafond Jonathan A. , Rousseau Alain N. , Gallichand Jacques , Caron Jean
Published: March 2022

This article proposes an analytical strategy that combines X-ray Computed Tomography (CT) and Continuous Wavelet Transform (CWT) analysis as an alternative solution to long-term experiments that seek to investigate spatiotemporal variations in soil hydraulic properties induced by drainage and recharge cycles. We conducted CT scanning on 100-cm-high column filled with two types of sandy soil in a laboratory environment to simulate, over the period of a month, the equivalent of nearly 40 years of drainage/recharge cycles akin to agricultural fields adopting subirrigation as water management practices. We also monitored soil matric potential, water inflow and outflow, as well as movement of tracers. This later consists in zirconium oxide (ZrO2) that we added to the top 20 cm of each soil column. The results revealed that drainage and recharge cycles greatly affect the evolution of soil hydraulic properties at different locations along the soil profile by reducing drainage and capillary capacities. The approach also allowed us to identify each periodic component of drainage and recharge cycles, and thereby calculate the periodic drift over time. The proposed method can be applied to predict soil evolution according to soil texture, drainage system design and water management, thereby offering a potential basis for proposing mitigation measures related to soil hydrodynamics. It may find its application in agricultural farms adopting subirrigation and surface (e.g., drip) irrigation approaches and, in mining and civil engineering.
Reconstruction of Large Full-Thickness Abdominal Wall Defects Using a Free Functional Latissimus Dorsi Muscle
Authors: Ninkovic Marijana , Ninkovic Marina , Öfner Dietmar , Ninkovic Milomir
Published: March 2022

Introduction

The large full-thickness abdominal wall defect has to be treated by considering anatomical and functional requirements. The abdominal wall must regain total physiological function, which means that the full thickness abdominal wall defect must be reconstructed anatomically, not only according to the anatomical requirements but also maintaining the functional dynamic voluntary movement. Defects in the abdominal wall alter respiratory mechanics and can impair the diaphragm function. Additionally, muscles of the anterolateral abdominal wall increase the stability of the lumbar region of the vertebral column by tensing the thoracolumbar fascia and by increasing intraabdominal pressure.

Materials and Methods

The timing and method of reconstruction must be chosen depending upon the etiology of the defect. Severe traumatic injuries, abdominal wall infections, necrotizing soft tissue loss, or sepsis needs to undergo staged reconstruction following adequate debridement to control the infectious process, establish the zone of injury, and for proper treatment of intraabdominal pathology, thereby achieving temporary primary closure using split-thickness skin grafting to the viscera. At the time of definitive reconstruction, deep skin graft dermabrasion give us a facial-like layer with adequate strength to stabilize the static abdominal wall. This dermal layer is supported by free functional (innervated) latissimus dorsi muscle (fLDM), giving full anatomical coverage and functional stability. After oncologic resections full-thickness abdominal wall reconstruction was performed immediately with a combination of fLDM flaps and meshes.

Results

A total of 14 patients underwent abdominal wall reconstruction using the fLDM flap. Staged reconstruction was applied in 8 cases. In the remaining six cases, two had no mesh support, three had synthetic mesh, and one had a fascial graft, which were covered with fLDM flap. There were no free flaps failure. One flap revision due to venous anastomosis thrombosis was performed. Donor site seromas occurred in 5 cases and were treated with punction and direct doxycycline injection. Electromyographic testing postoperatively confirmed reinnervation of transplanted LDM.

Conclusion

Using fLDM as a definitive solution, we are not only able to repair soft tissue defects, but also reconstruct voluntary contractility and dynamic natural functional abdominal wall. Transplanted LDM offers enough contractile capacity and strength to replace the function of the missing abdominal wall muscles.
A Feminist Ethic of Care for the Veterinary Profession
Authors: Ashall Vanessa
Published: March 2022

I can still see the dog's face as its eyes connected with mine, framed by the black bin bag it had been carried in. I can still hear the clicking sound, louder than the animal's shrill cries, made by a mass of maggots moving against one another beneath the dogs matted fur, moistened by fluids leaking from its damaged flesh. My hands were shaking with panic and rage and I could hardly draw up the euthatal into the syringe quickly enough. I wanted to put an end to this, immediately. As the lethal fluid flowed into the tiny vein the dog's body finally relaxed. At my hand, like so many others, she had ceased to exist. Through the window I could see her owners waiting outside in the sunshine to pay me and I thought about the silky feel of the fur which covered an expensively shaped head. I knew this dog was loved once.

This paper develops two neglected areas of veterinary thought; anthropological studies of the veterinary profession and feminist care approaches in veterinary ethics. I argue that the development of veterinary anthropology is crucial to advancing our understanding of veterinary lived experiences, through highlighting the previously under acknowledged emotional, relational and contextual realities of veterinary practice. I further propose that an ethic of care for the veterinary profession, which meaningfully connects with veterinary lived experiences, may provide a valuable approach through which to further develop veterinary ethical thinking. I share an autoethnographic account of a difficult veterinary encounter, which I then analyse using a novel feminist care approach. Through analyses centered on both emotional and relational aspects of veterinary care, I challenge the boundaries of traditional veterinary ethical approaches in terms of the scope, scale and complexity of veterinary ethical decision making. I describe the concept of emotional sponge work in veterinary practice and outline its potential impact for advancing understanding of both veterinary well-being and the profession's societal role. Finally, I propose that a feminist ethic of care might provide a framework for redefining the focus of veterinary professional responsibility, beyond animal health and toward the maintenance of healthy relationships between humans and animals.
Managing Shiga Toxin-Producing E. coli Using Statistical Process Control Charts for Routine Health and Production Monitoring in Pig Farming
Authors: Scollo Annalisa , Fasso Mattia , Nebbia Patrizia , Mazzoni Claudio , Cossettini Claudia
Published: March 2022

Oedema disease (ED) caused by Shiga-toxin-producingE. coliin pigs is a serious life-threatening disease, particularly among weaned piglets. When a preventive protocol is adopted in a specific farm, interpretation of effectiveness is often complicated in field conditions due to natural or “common cause” variation. For this reason, in this study a Statistical process control (SPC) approach was used to retrospectively evaluate the application of an ED preventive protocol (lower protein diet,ad-libitumfiber, vaccination at 5 days of age) in an infected commercial piglets' weaning site. The analysis was established over a 9-years period (n= 75 consecutive batches; 1,800 weaners per batch) using mortality for each batch as the key parameter of health and production; the statistics and the control limits (mean ± 3-fold sd; UCL, upper control limit; LCL, lower control limit) were based on data from the first 28 batches (Period 1) before the onset of the first ED clinical signs. The charts allowed the detection of defined out of control batches (i.e., with mortality out of the intervention limits) from batch 29 ongoing, exploring a Period 2 (unstable production and ED clinical signs; 36 batches) and a Period 3 (application of the ED preventive protocol; 11 batches). Mortality evaluation using SPC revealed a production system defined under-control (mean moving range bar = 1,34%; UCL = 4,37%; LCL = 0%) during Period 1. During Period 2, charts lost the state of statistical control, as showed by several signals of special cause variation due to the ED outbreak. Period 3 was characterized again by a state of statistical control, where no signals of special cause variation was showed. In conclusion, the retrospective application of SPC charts in the present study was able to confirm the efficacy of an ED preventive protocol in reducing mortality in a piglets' weaning site. SPC charting is suggested as an useful tool to provide insights into relationships between health, managerial, and welfare decision and some selected iceberg parameters in livestock.
Component Identification and Analysis of Vesicular Fluid From Swine Infected by Foot-and-Mouth Disease Virus
Authors: Zhang Ting , Lu Bingzhou , Yang Bo , Zhang Dajun , Shi Xijuan , Shen Chaochao , Cui Huimei , Yuan Xingguo , Zhao Dengshuai , Yang Jinke , Hao Yu , Chen Xuehui , Liu Xiangtao , Zhang Keshan , Zheng Haixue
Published: March 2022

Foot-and-mouth disease (FMD) is induced by FMD virus (FMDV) and characterized by fever and vesicular (blister-like) lesions. However, the exact composition of the vesicular fluid in pigs infected with FMDV remains unclear. To identify and analyze the components of the vesicular fluid in FMDV-infected domestic pigs, the fluid was collected and subjected to mass spectrometry. Further analyses were conducted using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genome (KEGG), and protein–protein interaction (PPI). Quantitative ELISA kit for TNF-α, and IFN-α, IFN-β, IL-6, IL-10, IL-1β, and IFN-γ were used to verify the mass spectrometry results. Results showed that 937 proteins were identified in the vesicular fluid from swine after FMDV infection, and bioinformatics analysis indicated that these proteins are related to the innate immune and inflammation pathways. The levels of cytokines involved in the disease-related pathways, tumor necrosis factors, and IL-6 in the fluid samples were significantly increased. This study identified and analyzed the composition of vesicular fluid in pigs after FMD infection for the first time and provided interesting information that help understand the infection and pathogenesis mechanism of FMD. These information will eventually contribute to the prevention and control of FMD.
Liver Transplantation as a Cornerstone Treatment for Acute-On-Chronic Liver Failure
Authors: Schulz Martin S. , Gu Wenyi , Schnitzbauer Andreas A. , Trebicka Jonel
Published: March 2022

Acute-on-chronic liver failure (ACLF) is a distinct clinical syndrome, characterized by acute decompensation (AD) of liver cirrhosis, severe systemic inflammation, intra- and extrahepatic organ failures, and a high short-term mortality. Liver transplantation (LT) is a potentially life-saving treatment for patients with decompensated liver cirrhosis and, due to the high mortality rates, particularly for ACLF patients. In the last decade, a plethora of studies has produced compelling evidence in favor of LT in ACLF, demonstrating high post-LT survival rates and excessive waitlist mortality. The importance of LT in these patients is underscored by the fact that no specific therapy for ACLF is available yet, rendering expeditious life-saving LT to be the only feasible treatment option for some ACLF patients. This review aims to provide an overview on pathophysiology, clinical trajectory, and clinical management of ACLF and to delineate the current literature regarding perspectives and limitations of LT as a life-saving treatment option for ACLF patients.
A magyar nyelv használata a romániai fogorvosi oktatásban
Authors: Székely Melinda , Matekovits György
Published: March 2022

Jelen írás célja, hogy áttekintést nyújtson a magyar nyelv használatáról a romániai fogorvosképzésben. Az első rész röviden ismerteti az első magyar nyelvű erdélyi orvosi könyv fogorvosi vonatkozásait. A második rész bemutatja az erdélyi fogorvosi oktatás történetét a kezdetektől napjainkig, különös tekintettel a magyar nyelv használatára. Az egyetemi szintű fogorvosi oktatás 1890-ben kezdődött a kolozsvári Magyar Királyi Ferenc József Tudományegyetemen mint fakultatív tantárgy az orvostanhallgatók számára. Az egyetem magyar tannyelvű graduális és posztgraduális fogorvosképzést biztosított az 1913–1919, valamint 1940–1945 közötti években, bár 1921–1940 között Szegedre költöztették. A kolozsvári egyetem, többszöri névváltoztatás után, Bolyai Tudományegyetem lett, és a magyar tannyelvű Orvostudományi Karát 1945-ben áthelyezték Marosvásárhelyre. Itt az 1948-as tanügyi reform következtében vált Marosvásárhelyi Orvosi és Gyógyszerészeti Intézetté, és létrehozták a Fogorvostudományi Kart, melynek működése megszűnt 1951 és 1960 között. A magyar nyelvű fogorvosképzést 1962-ben felváltotta a kétnyelvű oktatás: magyar és román nyelvű előadások voltak, de a gyakorlatokat csak román nyelven tartották. Jelenleg is így zajlik az oktatás, bár időközben Romániában rendszerváltozás volt, egyetemegyesítés, és többször változott az egyetem elnevezése. 2014-ben bevezették az angol nyelvű fogorvosképzést, ahol minden oktatási tevékenység angol nyelven zajlik. Végezetül megjelennek az egyéni gondolataink az anyanyelven történő oktatás és a magyar fogorvosi szaknyelv megőrzésének fontosságáról, valaminta román és angol szakkifejezések hatásáról. Összegezve, Romániában az egyedüli hely, ahol magyar nyelven történika fogorvostudomány (elméleti) oktatása, az a Marosvásárhelyi „George Emil Palade” Orvosi, Gyógyszerészeti, Tudomány- és Technológiai Egyetem Fogorvostudományi Kara.
Development of an Attitude Adjustment Crawler Chassis for Combine Harvester and Experiment of Adaptive Leveling System
Authors: Hu Jinpeng , Pan Jiahui , Dai Buwang , Chai Xiaoyu , Sun Yixin , Xu Lizhang
Published: March 2022

Body tilt is typically unavoidable for agricultural vehicles or platforms when operated over tough terrain in agricultural production, and, therefore, it is not possible to guarantee the operation performance. Current leveling methods mainly focus on wheeled vehicles or adjustment for single working parts rather than the vehicle body, and few of them could be applied to crawler vehicles. The objective of this research was to put forward an adaptive leveling system for combine harvesters based on the development of a new four-point lifting adjustable crawler chassis. The working principle of the proposed adjustable mechanism was studied on the basis of the analysis of adjustment characteristics under multiple posture adjustment conditions in a RecurDyn environment. The design relies on the combination of the attitude detection of the leveling system and adjustment calculation of driving hydraulic cylinders according to the established mathematical models. Within the designed adjustable extent (including adjustment range of vehicle height 0–87.8 mm, lateral inclination ±3.98° and longitudinal inclination −2.9–5.2°), the leveling performance of lateral and longitudinal inclination was then tested separately. The experiment showed that the leveling system could achieve automatic leveling with an accuracy of ±0.4° and could provide technical support for the development of crawler vehicles.
Optimizing the N Rate for Maize Forage to Balance Profits and N Ecological Stress
Authors: Han Kun , Liu Peng
Published: March 2022

The N application used for the production of forage affects environment stress. The N application rate used for the production of the whole maize (Zea mays L.) plant (WMP) affects the produced feed and environment. We carried out a 2-year field experiment aiming to measure how N application rates affect WMP forage production, and estimated the impact on the environment. Five N application treatments (0, 100, 200, 300 and 400 kg N ha−1) were included in our study. The results showed that N application improved forage yield, achieving a higher economic and ecological profit. After reaching a certain optimal rate, however, increasing the N rate further no longer increased the yield and quality of WMP forage, but instead greatly increased estimated N losses, thereby reducing ecological profits. The comprehensive benefit of the optimal N rate was ordered: the optimal N rate that maximized agronomic profit > that maximized economic profit > that maximized ecological benefit. The optimal N rate maximizing ecological profit was lower by 21% and 37% than that maximizing economic profit and forage yield, respectively. N application rates with the highest ecological profit (USD 2478 ha−1 in 2017 and USD 2448 ha−1 in 2018) were 248 and 245 kg N ha−1, respectively, in 2017 and 2018. The optimized N rate that maximized ecological profit maintained the economic profit while reducing N fertilizer input and associated N losses; it also carried a lower economic and ecological cost due to estimated N losses. Ecological criteria, which combine economic profit and economic losses due to their environmental impact, are more efficient than agronomic or economic criteria when used to provide guidance for WMP forage production. Therefore, in WMP forage production, optimizing N application rate by ecological criteria could maintain a high forage yield and economic profit, but greatly reduce input costs and ecological stress, maximizing ecological profit.
Multidimensional Relationships of Starch Digestibility with Physicochemical, Pasting and Textural Properties of 30 Rice Varieties
Authors: Hu Liqin , Cao Jialin , Liu Yu , Xiao Zhengwu , Zhang Mingyu , Chen Jiana , Cao Fangbo , Iqbal Anas , Abou-Elwafa Salah Fatouh , Huang Min
Published: March 2022

Consuming rice with low starch digestibility is beneficial for reducing the risk of diabetes. Several factors have been shown to influence starch digestibility, but the combined effects of these factors on starch digestibility have not been studied. We assessed multidimensional relationships between the glucose production rate (GPR) of cooked rice with 16 indexes, including physicochemical, pasting and textural properties in 30 rice varieties. The stepwise multiple regression analysis showed that amylose content (AC), gel consistency (GC) and pasting temperature (PT) were closely related to GPR. This relationship could be described by the equation: GPR = −0.080 AC + 0.008 GC + 0.034 PT + 0.720, with a determination coefficient of 0.84. The variation partitioning analysis further indicated that AC, GC and PT independently explained 36%, 5% and 4% of the GPR variation, respectively. The interaction of AC and GC explained 46% of the variation in GPR. This study identifies the key indexes (AC, GC and PT) affecting starch digestibility and quantifies contributions of these indexes to the variation in GPR. The finding of our study provides useful information for breeding and selecting rice varieties with low GPR.
Nutritional Profile of Donkey and Horse Meat: Effect of Muscle and Aging Time
Authors: Marino Rosaria , della Malva Antonella , Maggiolino Aristide , De Palo Pasquale , d’Angelo Francesca , Lorenzo Josè Manuel , Sevi Agostino , Albenzio Marzia
Published: March 2022

The aim of this study was to evaluate the effect of 14-day vacuum aging on the nutritional composition of donkey and horse meat. Longissimus Dorsi (LD), Semimebranosus (SM), and Rectus Femoris (RF), Semitendinosus (ST) muscles were sampled from each left carcass of 12 donkeys and 12 horses, respectively. Each muscle was divided into three sections, vacuum packaged, and stored at 2 °C for different aging times (1, 6, and 14 days). Fatty acids, amino acids, and cholesterol content were determined. SM exhibited higher levels of polyunsaturated fatty acids (PUFA) both in donkey and horse, whereas LD evidenced higher saturated (SFA) and monounsaturated (MUFA) fatty acids and lower cholesterol content in horse after 1, 6, and 14 days of storage. An aging effect was found only in donkey meat with higher saturated fatty acids and lower PUFA content at the end of the aging period. The highest value of essential amino acids has been displayed in SM an LD muscles from horse and donkey, respectively. Our results highlighted that equine meat, due to an excellent nutritional profile, represents a healthy alternative to traditionally consumed red meat. A different aging method could be used in donkey meat to preserve the high PUFA content.
Studies on the Reactions of Biapenem with VIM Metallo β-Lactamases and the Serine β-Lactamase KPC-2
Authors: Lucic Anka , Malla Tika R. , Calvopiña Karina , Tooke Catherine L. , Brem Jürgen , McDonough Michael A. , Spencer James , Schofield Christopher J.
Published: March 2022

Carbapenems are important antibacterials and are both substrates and inhibitors of some β-lactamases. We report studies on the reaction of the unusual carbapenem biapenem, with the subclass B1 metallo-β-lactamases VIM-1 and VIM-2 and the class A serine-β-lactamase KPC-2. X-ray diffraction studies with VIM-2 crystals treated with biapenem reveal the opening of the β-lactam ring to form a mixture of the (2S)-imine and enamine complexed at the active site. NMR studies on the reactions of biapenem with VIM-1, VIM-2, and KPC-2 reveal the formation of hydrolysed enamine and (2R)- and (2S)-imine products. The combined results support the proposal that SBL/MBL-mediated carbapenem hydrolysis results in a mixture of tautomerizing enamine and (2R)- and (2S)-imine products, with the thermodynamically favoured (2S)-imine being the major observed species over a relatively long-time scale. The results suggest that prolonging the lifetimes of β-lactamase carbapenem complexes by optimising tautomerisation of the nascently formed enamine to the (2R)-imine and likely more stable (2S)-imine tautomer is of interest in developing improved carbapenems.
Vitamin Supplements as a Nutritional Strategy against Chronic Alcohol Consumption? An Updated Review
Authors: Sandoval Cristian , Farías Jorge , Zamorano Mauricio , Herrera Christian
Published: March 2022

Several studies have shown that blood vitamin levels are low in alcoholic patients. In effect, alcohol use abuse is considered a chronic disease that promotes the pathogenesis of many fatal diseases, such as cancer and liver cirrhosis. The alcohol effects in the liver can be prevented by antioxidant mechanisms, which induces enzymatic as well as other nonenzymatic pathways. The effectiveness of several antioxidants has been evaluated. However, these studies have been accompanied by uncertainty as mixed results were reported. Thus, the aim of the present review article was to examine the current knowledge on vitamin deficiency and its role in chronic liver disease. Our review found that deficiencies in nutritional vitamins could develop rapidly during chronic liver disease due to diminished hepatic storage and that inadequate vitamins intake and alcohol consumption may interact to deplete vitamin levels. Numerous studies have described that vitamin supplementation could reduce hepatotoxicity. However, further studies with reference to the changes in vitamin status and the nutritional management of chronic liver disease are in demand.
H2O2-Responsive Hormonal Status Involves Oxidative Burst Signaling and Proline Metabolism in Rapeseed Leaves
Authors: Lee Bok-Rye , La Van Hien , Park Sang-Hyun , Mamun Md Al , Bae Dong-Won , Kim Tae-Hwan
Published: March 2022

Drought alters the level of endogenous reactive oxygen species (ROS) and hormonal status, which are both involved in the regulation of stress responses. To investigate the interplay between ROS and hormones in proline metabolism, rapeseed (Brassica napus L.) plants were exposed to drought or exogenous H2O2 (Exo-H2O2) treatment for 10 days. During the first 5 days, the enhanced H2O2 concentrations in drought treatment were associated with the activation of superoxide dismutase (SOD) and NADPH oxidase, with enhanced ABA and SA levels, while that in Exo-H2O2 treatment was mainly associated with SA-responsive POX. During the latter 5 days, ABA-dependent ROS accumulation was predominant with an upregulated oxidative signal-inducible gene (OXI1) and MAPK6, leading to the activation of ABA synthesis and the signaling genes (NCED3 and MYC2). During the first 5 days, the enhanced levels of P5C and proline were concomitant with SA-dependent NDR1-mediated signaling in both drought and Exo-H2O2 treatments. In the latter 5 days of drought treatment, a distinct enhancement in P5CR and ProDH expression led to higher proline accumulation compared to Exo-H2O2 treatment. These results indicate that SA-mediated P5C synthesis is highly activated under lower endogenous H2O2 levels, and ABA-mediated OXI1-dependent proline accumulation mainly occurs with an increasing ROS level, leading to ProDH activation as a hypersensitive response to ROS and proline overproduction under severe stress.