bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased Cerebello-Prefrontal Connectivity Predicts Poor Executive Function in Congenital Heart Disease
Authors: Sahel Aurelia , Ceschin Rafael , Badaly Daryaneh , Lewis Madison , Lee Vince K. , Wallace Julia , Weinberg Jacqueline , Schmithorst Vanessa , Lo Cecilia , Panigrahy Ashok
Published: August 2023

Background: Children and adolescents with congenital heart disease (CHD) are at risk for cognitive impairments, such as executive function deficits and motor delays, which can impact their academic and adaptive functioning as well as their quality of life. We investigated whether alterations in connectivity between the prefrontal and cerebellar brain structures exist between CHD and control cohorts and if these alterations could predict cognitive or motor impairment among youths with CHD. Methods: 53 participants with CHD and 73 healthy control participants completed multi-modal magnetic resonance imaging (MRI) of the brain, including high-resolution diffusion tensor imaging at 3T. We measured connectivity from masked regions of interest in the cerebellum to the frontal cortex using a probabilistic tractography method. Participants also completed neuropsychological tests of cognitive and motor skills using the NIH Toolbox. Results: In the CHD group, fractional anisotropy (FA) was increased in the cognitive loop connectivity pathways, including from the right cerebellum to the left thalamus (p = 0.0002) and from the left thalamus to the left medial frontal gyrus (MFG) (p = 0.0048) compared with the healthy control group. In contrast, there were no differences between CHD and controls in motor loop connectivity pathways. An increase in FA from the right thalamus to the MFG tract in the cognitive loop (posterior subdivision) predicted (p = 0.03) lower scores on the NIHTB tests, including those of executive functioning. A transient increase in connectivity of the cognitive loop in the adolescent group was observed relative to the child and adult groups. Conclusions: Our results suggest that selective alteration of cerebellum-cerebral connectivity circuitry within the cognitive loops predicts cognitive dysfunction in CHD youth. Our study suggests a critical period of cerebellar circuitry plasticity in the adolescent period in CHD subjects that drives neurocognitive function. Further replication and validation in other pediatric CHD cohorts is warranted for future work.
Italian Physicians’ Perceptions about the Role of Asciminib in Later Lines Chronic Myeloid Leukemia in Clinical Practice: A GIMEMA Survey
Authors: Breccia Massimo , Piciocchi Alfonso , Abruzzese Elisabetta , Cilloni Daniela , Messina Monica , Soddu Stefano , Castagnetti Fausto , Stagno Fabio , Fazi Paola , Iurlo Alessandra , Caocci Giovanni , Gozzini Antonella , Intermesoli Tamara , D’Adda Mariella , Pane Fabrizio
Published: August 2023

Unmet needs remain in later lines chronic myeloid leukemia (CML): the response rate and the overall survival of resistant patients in the chronic phase who changed a second-generation TKI in the second line with another TKI with similar action are usually poor, while the off-target toxicities and the potential development of mutations increase. The recent approval of asciminib, a STAMP inhibitor, in the third line, has the potential to soon change the therapeutic algorithm for this subset of patients. Here, we report the results of a GIMEMA survey assessing the number of patients currently treated in the third line in Italy, the current approach in later lines by Italian physicians, and the future role of this drug according to the reason to switch to asciminib (resistance and/or intolerance), as well as the perceptions about the future position of this agent.
Special Issue “Sleep Disorders: From Clinical Research to Daily Practice”
Authors: Fisicaro Francesco , Lanza Giuseppe , Figorilli Michela
Published: August 2023

Healthy sleep is indissolubly linked to both physical and mental health, as pointed out by evidence showing the negative impact of poor sleep on neurological, psychiatric, cardiovascular, respiratory, metabolic, and immune systems, among others [...]
Recurrent Anterior Shoulder Instability Treated Using the Arthroscopic Bankart–Latarjet Technique: Experience of a Peripheral Hospital
Authors: Fouasson-Chailloux Alban , Estoppey Daniel , Perdreau Alan , Bessière Charles , Goldstein Yariv , Duysens Christophe
Published: August 2023

The arthroscopic Bankart–Latarjet procedure is used in the surgical management of anterior shoulder instability. This technique is mainly performed in referral centers due to its high technicity. This study aimed to evaluate surgical outcomes in a peripheral hospital center. This is a retrospective study of patients treated for recurrent anterior shoulder instability. The clinical scores (Walch–Duplay, Rowe, and Western Ontario Shoulder Instability Index (WOSI)) were assessed preoperatively and at 12 months after surgery. The consolidation and the position of the bone block were evaluated at 6 months using a CT scan. Between 2016 and 2020, 40 patients had been operated on (mean age: 28.5 ± 7.9 years). During a mean follow-up of 29.5 ± 11.6 months, we noted only one complication, a case of fracture of the callus of a consolidated bone block. No recurrence of instability was recorded. The Walch–Duplay score increased from 17.8 to 94.6, the Rowe score from 24.9 to 96.8, and the WOSI score decreased from 52.1% to 6.9%. The bone block was consolidated in 35 patients (87.5%), and a flush position with the anterior edge of the glenoid was noted for all patients. At one year, 67.0% of the patients practicing sport had returned to sports. The arthroscopic Bankart–Latarjet technique was a reliable procedure in the hands of an experienced shoulder surgeon, even in a peripheral hospital center.
Efficacy of Non-Enhanced Brain Computed Tomography in Patients Presenting to the Emergency Department with Headache after COVID-19 Vaccination
Authors: Lee Yongtack , Kim Kyuseok , Paek So-Hyun , Chang Hyunglan
Published: August 2023

Headaches are a common side effect of vaccination against the severe acute respiratory syndrome, coronavirus 2; however, it is usually not necessary to seek emergency medical attention or undergo brain imaging such as non-enhanced brain computed tomography (CT) for routine evaluation of vaccine-related headaches. This study aimed to demonstrate that brain CT is of no clinical benefit to patients presenting to the emergency department (ED) with post-coronavirus disease 2019 (COVID-19) vaccination headaches. This retrospective, single-center observational study used electronic medical record (EMR) data of patients who received the COVID-19 vaccination during the first year of the vaccination program. In total, 914 patients were analyzed, of whom 435 underwent CT (CT group, n = 435; no CT group, n = 475). More female patients visited the ED, and there was no significant sex difference between the CT and no-CT groups. The type of vaccine affected the clinical decision to perform brain CT, but the number of doses did not. The CT rate was relatively high for patients who had received the ChAdOx1 nCoV-19 (Oxford–AstraZeneca) and Johnson and Johnson Janssen (Jansen) vaccines (p = 0.004). Focal neurological deficits were present in all cases of abnormalities on non-enhanced brain CT in patients complaining of headaches. Two out of the 435 patients had abnormal brain CT findings (glioblastoma and Rathke’s pouch cyst) at 35 and 32 days after vaccination, respectively. Non-enhanced brain CT should be performed cautiously in patients visiting the ED for post-vaccination headaches only.
Proliferative Vitreoretinopathy: A Reappraisal
Authors: Carpineto Paolo , Licata Arturo Maria , Ciancaglini Marco
Published: August 2023

Proliferative vitreoretinopathy (PVR) remains the main cause of failure after retinal detachment (RD) surgery. Despite the development of modern technologies and sophisticated techniques for the management of RD, the growth of fibrocellular membranes within the vitreous cavity and on both sides of the retinal surface, as well as intraretinal fibrosis, can compromise surgical outcomes. Since 1983, when the term PVR was coined by the Retina Society, a lot of knowledge has been obtained about the physiopathology and risk factors of PVR, but, despite the proposal of a lot of therapeutic challenges, surgical skills seem to be the only effective way to manage PVR complications.
Impact of Dental Anxiety on Dental Care Routine and Oral-Health-Related Quality of Life in a German Adult Population—A Cross-Sectional Study
Authors: Winkler Christian H. , Bjelopavlovic Monika , Lehmann Karl M. , Petrowski Katja , Irmscher Lisa , Berth Hendrik
Published: August 2023

The interaction between dental anxiety and the establishment of a consistent dental care routine has been an ongoing challenge. Unfortunately, there is limited available data concerning the detailed dental care practices of individuals with dental anxiety. Therefore, this study aims to explore how dental anxiety influences dental care habits and oral-health-related quality of life within an adult population. By utilizing the Dental Anxiety Scale (DAS) and the German Oral Health Impact Profile (OHIP-G5), we assessed their extent. To evaluate the differences, we performed analyses of variance (Anova), an independent t-test and rank correlation. The findings of this study unveil a significant correlation between elevated DAS scores and reduced frequency of tooth brushing; calculus removal and appointments for professional teeth cleaning. Interestingly; the use of dental floss and mouthwash solution as well as toothbrush hardness appeared to be not significantly affected by dental fear. Moreover, individuals with dental anxiety demonstrated a preference for manual toothbrushes over electric ones. In addition, higher DAS scores were found to be strongly associated with greater OHIP-G5 scores, thus leading to a substantial decline in overall oral health-related quality of life.
Exploring Current Concepts and Challenges in the Identification and Management of Early-Stage COPD
Authors: Doña Esperanza , Reinoso-Arija Rocío , Carrasco-Hernandez Laura , Doménech Adolfo , Dorado Antonio , Lopez-Campos José Luis
Published: August 2023

The need to improve health outcomes, as well as disease prognosis, has led clinicians and researchers to propose new ways of identifying COPD in its earliest forms. This initiative is based on the hypothesis that an earlier intervention would have a greater prognostic impact. However, the operational definition of a patient in the initial stages of the disease is complex, and there is still no unanimously accepted definition. GOLD has recently proposed different concepts to identify COPD in its early stages, such as COPD in young people or COPD with mild functional impairment. In addition, GOLD proposes two other concepts, called pre-COPD (symptomatic non-obstructive patients) and PRISm (preserved ratio with impaired spirometry), which aim to identify the patient at risk of developing this chronic airflow obstruction. However, despite the attractiveness of these concepts, none have been taken up universally by the medical community. A universally accepted identification of how to define COPD in its early stages is necessary as a preliminary step in order to design clinical trials to find out the best way to treat these patients. This review deals with these concepts of COPD at the onset of the disease, highlighting their importance and the problems involved in identifying them as therapeutic targets in real clinical practice.
Store-Operated Ca2+ Entry as a Putative Target of Flecainide for the Treatment of Arrhythmogenic Cardiomyopathy
Authors: Moccia Francesco , Brunetti Valentina , Soda Teresa , Faris Pawan , Scarpellino Giorgia , Berra-Romani Roberto
Published: August 2023

Arrhythmogenic cardiomyopathy (ACM) is a genetic disorder that may lead patients to sudden cell death through the occurrence of ventricular arrhythmias. ACM is characterised by the progressive substitution of cardiomyocytes with fibrofatty scar tissue that predisposes the heart to life-threatening arrhythmic events. Cardiac mesenchymal stromal cells (C-MSCs) contribute to the ACM by differentiating into fibroblasts and adipocytes, thereby supporting aberrant remodelling of the cardiac structure. Flecainide is an Ic antiarrhythmic drug that can be administered in combination with β-adrenergic blockers to treat ACM due to its ability to target both Nav1.5 and type 2 ryanodine receptors (RyR2). However, a recent study showed that flecainide may also prevent fibro-adipogenic differentiation by inhibiting store-operated Ca2+ entry (SOCE) and thereby suppressing spontaneous Ca2+ oscillations in C-MSCs isolated from human ACM patients (ACM C-hMSCs). Herein, we briefly survey ACM pathogenesis and therapies and then recapitulate the main molecular mechanisms targeted by flecainide to mitigate arrhythmic events, including Nav1.5 and RyR2. Subsequently, we describe the role of spontaneous Ca2+ oscillations in determining MSC fate. Next, we discuss recent work showing that spontaneous Ca2+ oscillations in ACM C-hMSCs are accelerated to stimulate their fibro-adipogenic differentiation. Finally, we describe the evidence that flecainide suppresses spontaneous Ca2+ oscillations and fibro-adipogenic differentiation in ACM C-hMSCs by inhibiting constitutive SOCE.
The Impact of Nitroglycerin on the Evaluation of Coronary Stenosis in Coronary-CT: Preliminary Study in 131 Patients
Authors: D’Errico Francesca , Ricci Francesca , Luciano Alessandra , Sbordone Francesco Paolo , Laudazi Mario , Mecchia Daniele , Volpe Maria , Briganti Flavia , Di Landro Alessio , Muscoli Saverio , Pugliese Luca , De Stasio Vincenzo , Di Donna Carlo , Romeo Francesco , Garaci Francesco , Floris Roberto , Chiocchi Marcello
Published: August 2023

Background: The sublingual administration of short-acting nitroglycerin (NTG) before coronary computed tomography (CCT) improves the visualization of coronary arteries, causing vasodilatation. The aim of this study was to evaluate whether and how nitroglycerin can influence the concordance between radiologists and cardiologists in the evaluation of vessel stenosis measured in CCT by the former and during the following coronarography by the latter. Methods: We conducted a retrospective analysis of 131 patients who underwent CCT for cardiac symptoms in 2022, followed by coronarography performed six months later because of significant stenosis revealed by the CCT. First, the patients were divided into two groups: an NTG group who received sublingual nitroglycerin before CCT and a non-NTG group who did not because of contraindications. Second, 254 stenoses were measured by two radiologists after CCT and by two interventional cardiologists during the next coronarography; moreover, stenoses were classified on the basis of their location and plaque pattern (calcific, mixed and lipidic). Third, the strength of agreement was evaluated between the two radiologists, between the two cardiologists and finally between the radiologists and cardiologists in order to evaluate whether and how the interdisciplinary discrepancy in stenosis evaluation could change with or without the use of nitroglycerin before CCT and in relation to the different plaque pattern. Results: In the NTG group, the use of nitroglycerine reduced the agreement between radiologists and cardiologists in calcific stenosis but did not change the concordance in the case of mixed or lipidic plaques on the same vessels. Conclusions: The use of sublingual nitroglycerin before CCT may lead to a radiological overestimation of calcific stenosis.
Sex Difference on Neurological Outcomes and Post-Cardiac Arrest Care in Out-of-Hospital Cardiac Arrest Patients Treated with Targeted Temperature Management: Post-Hoc Study of a Prospective, Multicenter, Observational Cohort Study
Authors: Park Seon Yeong , Oh Sang Hoon , Park Sang Hyun , Oh Jae Hun , Kim Soo Hyun
Published: August 2023

Conflicting results regarding sex-based differences in the outcomes of out-of-hospital cardiac arrest (OHCA) patients have been reported. We aimed to evaluate the association between sex and neurological outcome as well as various in-hospital process in OHCA patients treated with targeted temperature management. We retrospectively analyzed a prospective registry data collected between October 2015 and December 2018. To evaluate the effect of sex on patient outcomes, we created various multivariable logistic regression models. When the results were adjusted using resuscitation variables and in-hospital variables, there was no significant difference (OR = 1.22, 95% CI: 0.85–1.74; OR = 1.13, 95 CI: 0.76–1.68, respectively). Regarding the in-hospital course, the daily total SOFA score was similar in both sexes, whereas cardiovascular scores were higher in women on days 2 and 3. The adjusted effect of sex was not associated with the clinician’s decision to perform early cardiac interventions, except for those men that had more extracorporeal membrane oxygenation (OR = 2.51, 95% CI: 1.11–5.66). The findings seems that men had more favorable 6-month neurological outcomes. However, after adjusting for confounders, there was no difference between the sexes. The results regarding in-hospital course were similar in men and women.
Immunolocalization of Some Epidermal Proteins and Glycoproteins in the Growing Skin of the Australian Lungfish (Neoceratodus forsteri)
Authors: Alibardi Lorenzo
Published: August 2023

Here we report the immunolocalization of mucin, nestin, elastin and three glycoproteins involved in tissue mineralization in small and large juveniles of Neoceratodus forsteri. Both small and larger juvenile epidermis are mucogenic and contain a diffuse immunolabeling for nestin. Sparse PCNA-labeled cells, indicating proliferation, are found in basal and suprabasal epidermal layers. No scales are formed in small juveniles but are present in a 5 cm long juvenile and in larger juveniles. Elastin and a mineralizing matrix are localized underneath the basement membrane of the tail epidermis where lepidotriches are forming. The latter appears as “circular bodies” in cross sections and are made of elongated cells surrounding a central amorphous area containing collagen and elastin-like proteins that undergo calcification as evidenced using the von Kossa staining. However, the first calcification sites are the coniform teeth of the small juveniles of 2–3 cm in length. In the superficial dermis of juveniles (16–26 cm in length) where scales are formed, the spinulated outer bony layer (squamulin) of the elasmoid scales contains osteonectin, alkaline phosphatase, osteopontin, and calcium deposits that are instead absent in the underlying layer of elasmodin. In particular, these glycoproteins are localized along the scale margin in juveniles where scales grow, as indicated by the presence of PCNA-labeled cells (proliferating). These observations suggest a continuous deposition of new bone during the growth of the scales, possibly under the action of these mineralizing glycoproteins, like in the endoskeleton of terrestrial vertebrates.
Associations of Pain Vigilance and Past and Current Pain with Kinesiophobia after Sport Injury in Current and Former Athletes from Iran and the United States
Authors: Badiei Fahimeh , Brewer Britton W. , Van Raalte Judy L.
Published: August 2023

High levels of kinesiophobia (fear of movement/reinjury) have been related to reinjury and adverse injury rehabilitation outcomes in athletes. To examine the extent to which pain vigilance, memory of injury-related pain, and current injury-related pain were associated with kinesiophobia, a cross-sectional study was conducted with 172 current and former athletes from Iran (n = 113) and the United States (n = 59) who reported having experienced a serious injury that affected their participation or performance in sport. Questionnaires were administered to participants via an online survey platform. Hierarchical multiple regression analysis revealed that pain vigilance and memory of pain were positively associated with kinesiophobia, with the full model accounting for 31% of the variance in kinesiophobia scores. The findings suggest that excessive attention to pain-related stimuli and memory of pain for an injury that occurred an average of four years earlier may contribute to the experience of fear of movement and reinjury in current and former athletes.
Comparison of Match External Loads across a Men’s and Women’s Lacrosse Season
Authors: Fields Jennifer B. , Jagim Andrew R. , Kuhlman Nicholas , Feit Mary Kate , Jones Margaret T.
Published: August 2023

The purpose of this study was to compare external workloads between collegiate men’s (MLAX) and women’s lacrosse (WLAX) matches and examine positional differences across the season. Athletes (MLAX: n = 10; WLAX: n = 13) wore a global positional system device during all matches. External load metrics included in the analysis were total distance (TD), sprint distance (SD), accelerations (>3 m/s2), sprint efforts, player load per minute (PL/min), top speed, and distances spent in various speed zones. WLAX had higher TD (p = 0.001), SD (p < 0.001), distances in SZs 2–5 (p < 0.001), PL (p < 0.001), and sprint efforts (p < 0.001) compared to MLAX. However, MLAX performed more acceleration (p < 0.001) and deceleration (p < 0.001) efforts. WLAX midfielders (M) and defenders (D) reached higher top speeds and performed more accelerations than attackers (p < 0.001). Midfielders covered the greatest distance at high speeds (p = 0.011) and the smallest distance at low speeds (<0.001) for WLAX. For MLAX, midfielders performed the highest SDs, top speeds, accelerations, decelerations, and distances in higher speed zones (p < 0.001) compared to attackers and defenders. Results indicate that there are significant gender and positional differences in external workload demands during match play, specifically for volume- and intensity-derived workload parameters, between men’s and women’s lacrosse. Therefore, sports performance coaches should create gender- and position-specific conditioning programs to prepare athletes for match demands.
Semi-Continuous Functionally Graded Material Austenitic to Super Duplex Stainless Steel Obtained by Laser-Based Directed Energy Deposition
Authors: Pereira Juan Carlos , Aguilar David , Tellería Iosu , Gómez Raul , San Sebastian María
Published: August 2023

In this work, a semi-continuous functionally graded material (FGM) between an austenitic and a super duplex stainless steel was obtained. These materials are of great interest for the chemical, offshore, and oil and gas sectors since the austenitic stainless steel type 316L is common (and not so expensive) and super duplex stainless steels have better mechanical and corrosion resistance but are more expensive and complex in their microstructural phases formation and the obtention of the balance between their main phases. Using directed energy deposition, it was possible to efficiently combine two powders of different chemical compositions by automated mixing prior to their delivery into the nozzle, coaxially to the laser beam for melting. A dense material via additive manufacturing was obtained, with minimum defectology and with a semi-continuous and controlled chemical compositional gradient in the manufactured part. The evolution of ferrite formation has been verified and the phase fraction measured. The resulting microstructure, austenite/ferrite ratio, and hardness variations were evaluated, starting from 100% austenitic stainless-steel composition and with variants of 5% in wt.% until achieving 100% of super duplex steel at the end of the part. Finally, the correlation between the increase in hardness of the FGM with the increase in the ferrite phase area fraction was verified.
Research on Multi-Target Path Planning for UUV Based on Estimated Path Cost
Authors: Zhou Shuai , Wang Zheng , Li Longmei , Li Houpu
Published: August 2023

The precision and efficiency of multi-target path planning are crucial factors influencing the performance of anti-mine operations using unmanned underwater vehicles (UUVs). Addressing the inadequacies in computation time and solution quality present in existing path planning algorithms, this study proposes a novel path cost estimation strategy based on neural networks. This strategy swiftly generates an accurate cost matrix, ensuring the attainment of high-quality traversal orders when utilized as input for the traveling salesman problem, thereby yielding a globally optimal path. Simulation experiments demonstrate that while maintaining high-quality solutions, the proposed strategy significantly enhances the computational efficiency of the algorithm. Furthermore, the practical application and effectiveness of the proposed algorithm have been demonstrated through an actual UUV prototype experiment in a lake environment.
Surface and Underwater Acoustic Source Recognition Using Multi-Channel Joint Detection Method Based on Machine Learning
Authors: Yu Qiankun , Zhu Min , Zhang Wen , Shi Jian , Liu Yan
Published: August 2023

Sound source recognition is a very important application of passive sonar. How to distinguish between surface and underwater acoustic sources has always been a challenge. Due to the mixing of underwater target radiated noise and marine environmental noise, especially in shallow water environments where multipath effects exist, it is difficult to distinguish them. To solve the surface and underwater acoustic source recognition problem, this paper proposes a multi-channel joint detection method based on machine learning. First, the simulation data are generated using the normal model KRAKEN setting in the same environment as the SACLANT 1993 experiment, which uses a vertical linear array of 48 hydrophones. Secondly, the GBDT classifier and LightGBM classifier are trained separately, and then the model is evaluated using precision, recall, F1, and accuracy. Finally, four ML models (kNN, random subspace kNN, GBDT, and LightGBM) are used to analyze all 48 channels of hydrophone data. For each model, two kinds of feature extraction methods (module features, real and imaginary features) are applied. Generally, the results show that both GBDT and LightGBM models have better performance than both kNN and random subspace kNN ones. For both GBDT and LightGBM models, the results using module features have better performance than using real and imaginary features.
Torsional Optical Fiber Stress Analysis and Vortex-Induced Vibration Study of Three-Core Submarine Cable
Authors: Tan Haotian , Hao Yanpeng , Zhang Peng , Li Qishun , Tian Wanxing , Chen Linhao , Yang Lin , Li Licheng
Published: August 2023

Due to current scouring, submarine cables are prone to be exposed, suspended, and even vortex-induced vibration, which is not conducive to the safe operation of the power grid. In this contribution, the finite element simulation model of a 35 kV three-core optical fiber composite submarine cable with a suspended span length of 9.5 m is established. The natural frequency of the model is obtained through modal analysis. Then the vortex-induced vibration is simulated by the fluid–structure coupling method, and the stress distribution and change law of the torsional optical fiber is extracted. The results show that in the submarine cable, there appears to be a beating vibration and locking phenomena respectively, under two flow velocities. The transverse vibration amplitude of the latter increases significantly due to a resonance state. When the flow direction is perpendicular to the submarine cable, the stress distribution of the torsional optical fiber at the initial moment and at the 1/2 T moment of a vibration cycle approximately represents a mirror image relationship. In addition, the frequency of the stress change is the same as the frequency of the vortex-induced vibration, which can judge whether the vortex-induced vibration occurs. Moreover, the vortex-induced vibration range can be determined by the maximum stress change location.
Advances in Wave Energy Conversion with Data-Driven Models
Authors: Clemente Daniel , Rosa-Santos Paulo , Taveira-Pinto Francisco
Published: August 2023

With an estimated theoretical resource of over 30,000 TWh/yr [...]
Towards Estimating Probability of Fish–Turbine Encounter: Using Drifters Equipped with Acoustic Tags to Verify the Efficacy of an Array of Acoustic Receivers
Authors: Sanderson Brian G. , Karsten Richard H. , Hasselman Daniel J.
Published: August 2023

An area has been designated for demonstrating the utility of marine hydrokinetic turbines in Minas Passage, Bay of Fundy. Marine renewable energy may be useful for the transition from carbon-based energy sources, but there is concern for the safety of fish that might encounter turbines. Acoustic receivers that detect signals from acoustically tagged fish that pass through the tidal demonstration area and the detection efficiency of tag signals might be used to estimate the likelihood of fish encountering marine hydrokinetic turbines. The method requires that tagged fish passing through the development area will be reliably detected by a receiver array. The present research tests the reliability with which passing tags are detected by suspending tags beneath GPS-tracked drifters. Drifters carrying high residency Innovasea tags that transmitted every 2 s were usually detected by the receiver array even in fast currents during spring tides but pulse-position modulation tags were inadequate. Sometimes very few high residency tag signals were detected when fast tidal currents swept a drifter through the receiver array, so increasing the transmission interval degrades performance at the tidal energy development area. High residency tags suspended close to the sea surface were slightly less likely to be detected if they passed by during calm conditions. Previously measured detection efficiencies were found to slightly overestimate the chances of a high residency tag carried by a drifter being detected as it passed by a receiver. This works elucidates the effectiveness with which acoustically tagged fish are detected in fast, highly turbulent tidal currents and informs the application of detection efficiency measurements to calculate the probability that fish encounter a marine hydrokinetic turbine.
A Multiplex PCR Melting-Curve-Analysis-Based Detection Method for the Discrimination of Five Aspergillus Species
Authors: Tokamani Maria , Figgou Eleftheria , Papamichail Lito , Sakka Eleni , Toros Athanasios , Bouchorikou Anastasia , Giannakakis Antonis , Matthaiou Efthymia Iliana , Sandaltzopoulos Raphael
Published: August 2023

Aspergillus mold is a ubiquitously found, airborne pathogen that can cause a variety of diseases from mild to life-threatening in severity. Limitations in diagnostic methods combined with anti-fungal resistance render Aspergillus a global emerging pathogen. In industry, Aspergilli produce toxins, such as aflatoxins, which can cause food spoilage and pose public health risk issues. Here, we report a multiplex qPCR method for the detection and identification of the five most common pathogenic Aspergillus species, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Aspergillus nidulans. Our approach exploits species-specific nucleotide polymorphisms within their ITS genomic regions. This novel assay combines multiplex single-color real time qPCR and melting curve analysis and provides a straight-forward, rapid, and cost-effective detection method that can identify five Aspergillus species simultaneously in a single reaction using only six unlabeled primers. Due to their unique fragment lengths, the resulting amplicons are directly linked to certain Aspergillus species like fingerprints, following either electrophoresis or melting curve analysis. Our method is characterized by high analytical sensitivity and specificity, so it may serve as a useful and inexpensive tool for Aspergillus diagnostic applications both in health care and the food industry.
Clinical, Epidemiological and Laboratory Features of Invasive Candida parapsilosis Complex Infections in a Brazilian Pediatric Reference Hospital during the COVID-19 Pandemic
Authors: Peixoto Paulo Henrique , Silva Maria Laína , Portela Fernando Victor , da Silva Bruno , Milanez Edlâny , de Oliveira Denis , Ribeiro Aldaíza , de Almeida Henrique , Lima-Neto Reginaldo , Guedes Glaucia Morgana , Castelo-Branco Débora , Cordeiro Rossana
Published: August 2023

The present study aimed to describe the clinical, epidemiological and laboratory characteristics of invasive candidiasis by C. parapsilosis complex (CPC) in a Brazilian tertiary pediatric hospital during the COVID-19 pandemic. Clinical samples were processed in the BACT/ALERT® 3D system or on agar plates. Definitive identification was achieved by MALDI-TOF MS. Antifungal susceptibility was initially analyzed by the VITEK 2 system (AST-YS08 card) and confirmed by the CLSI protocol. Patient data were collected from the medical records using a structured questionnaire. CPC was recovered from 124 patients over an 18-month period, as follows: C. parapsilosis (83.87%), C. orthopsilosis (13.71%) and C. metapsilosis (2.42%). Antifungal resistance was not detected. The age of the patients with invasive CPC infections ranged from <1 to 18 years, and most of them came from oncology-related sectors, as these patients were more affected by C. parapsilosis. C. orthopsilosis infections were significantly more prevalent in patients from critical care units. Invasive infections caused by different pathogens occurred in 75 patients up to 30 days after the recovery of CPC isolates. Overall, 23 (18.55%) patients died within 30 days of CPC diagnosis. Catheter removal and antifungal therapy were important measures to prevent mortality. COVID-19 coinfection was only detected in one patient.
Retrospective Analysis of the Airway Space Changes in Dentofacial Deformity after Two-Jaw Orthognathic Surgery Using Cone Beam Computed Tomography
Authors: Ravelo Víctor , Olate Gabriela , Unibazo Alejandro , de Moraes Márcio , Olate Sergio
Published: August 2023

Orthognathic surgery is used to modify anomalies in maxillomandibular position; this process can significantly affect the anatomy of the airway and cause functional changes. This study aims to define the impact of mandibular maxillary movement on the airway of subjects with dentofacial deformity. A retrospective study was conducted on subjects with Angle class II (CII group) and Angle class III (CIII group) dentofacial deformities. The subjects were treated via bimaxillary surgery; for all of them, planning was performed with software and 3D printing. Cone beam computed tomography (CBCT) was obtained 21 days before surgery and 6 months after surgery and was used for planning and follow-up with the same conditions and equipment. Was used the superimposition technique to obtain the maximum and minimum airway areas and total airway volume. The data were analyzed with the Shapiro–Wilk test and Student’s t-test, while Spearman’s test was used to correlate the variables, considering a value of p < 0.05. Thus, 76 subjects aged 18 to 55 years (32.38 ± 10.91) were included: 46 subjects were in CII group, treated with a maxillo-mandibular advancement, and 30 subjects were in the CIII group, treated with a maxillary advancement and a mandibular setback. In the CII group, a maxillary advancement of +2.45 mm (±0.88) and a mandibular advancement of +4.25 mm (±1.25) were observed, with a significant increase in all the airway records. In the CIII group, a maxillary advancement of +3.42 mm (±1.25) and a mandibular setback of −3.62 mm (±1.18) were noted, with no significant changes in the variables measured for the airway (p > 0.05). It may be concluded that maxillo-mandibular advancement is an effective procedure to augment the airway area and volume in the CII group. On the other hand, in subjects with mandibular prognathism and Angle class III operated with the maxillary advancement and mandibular setback lower than 4 mm, it is possible to not reduce the areas and volume in the airway.
The Role of Osteopontin in Respiratory Health and Disease
Authors: Barkas Georgios I. , Kotsiou Ourania S.
Published: August 2023

The biological functions of osteopontin (OPN) are diverse and specific to physiological and pathophysiological conditions implicated in inflammation, biomineralization, cardiovascular diseases, cellular viability, cancer, diabetes, and renal stone disease. We aimed to present the role of OPN in respiratory health and disease. OPN influences the immune system and is a chemo-attractive protein correlated with respiratory disease severity. There is evidence that OPN can advance the disease stage associated with its fibrotic, inflammatory, and immune functions. OPN contributes to eosinophilic airway inflammation. OPN can destroy the lung parenchyma through its neutrophil influx and fibrotic mechanisms, linking OPN to at least one of the two major chronic obstructive pulmonary disease phenotypes. Respiratory diseases that involve irreversible lung scarring, such as idiopathic pulmonary disease, are linked to OPN, with protein levels being overexpressed in individuals with severe or advanced stages of the disorders and considerably lower levels in those with less severe symptoms. OPN plays a significant role in lung cancer progression and metastasis. It is also implicated in the pathogenesis of pulmonary hypertension, coronavirus disease 2019, and granuloma generation.
Dual Perspectives on Financial Performance: Analyzing the Impact of Digital Transformation and COVID-19 on European Listed Companies
Authors: Mahssouni Rabie , Makhroute Mohamed , Touijer Mohamed Noureddine , Elabjani Abdelaziz
Published: August 2023

This paper conducts an analysis of the impact of COVID-19 and digital transformation (DT) on the financial performance of European listed companies. Using a panel data regression model from 2015 to 2021, the study analyzed the financial performance of 2179 companies. The sample of companies was chosen based on the availability of financial statements and aimed to examine the effects of COVID-19 and DT on financial performance, as measured by return on assets (ROA). The study used a fixed-effect model and checked for robustness by introducing return on equity (ROE) as a dependent variable. The results indicated that COVID-19 had a negative significant impact on financial performance, while DT had a positive significant impact, consistent with previous research. This study provides valuable insights into the impacts of the COVID-19 pandemic and DT on the financial performance of listed companies.
Designing Cultural Routes as a Tool of Responsible Tourism and Sustainable Local Development in Isolated and Less Developed Islands: The Case of Symi Island in Greece
Authors: Fafouti Aspasia E. , Vythoulka Anastasia , Delegou Ekaterini T. , Farmakidis Nikolaos , Ioannou Maria , Perellis Komninos , Giannikouris Antonis , Kampanis Nikolaos A. , Alexandrakis George , Moropoulou Antonia
Published: August 2023

Responsible tourism practices promote the idea that responsibility refers to both visitors and local communities, ensuring the protection and conservation of the natural environment and cultural heritage and contributing to a better quality of life. The role of “sustainability” in a tourist development strategy is to protect and reveal what already exists (“reuse, recycle, renovate”) rather than consume. In these terms, cultural routes can serve as valuable tourism assets that can attract visitors and generate economic activity, while protecting the environmental and cultural heritage. This study is focused on Symi, a small and remote Greek island better known as a religious tourism destination, examining how cultural routes could be at the core of an economic development and social cohesion strategic plan. Symi served as a case study due to the island’s rich cultural and natural heritage, including historic buildings, natural attractions, monuments, and archaeological sites. The revealing of those assets is crucial for the advancement of an integrated local strategy for cultural tourism in terms of responsible tourism. The primary objective of this study is to analyze how the development of a cultural routes network, integrating monuments from diverse historical periods, significant religious sites, and areas of significant architectural and environmental value, can contribute to a responsible tourism approach and further a sustainable development that generates multiple benefits for the local community in an insular region.
The Financial Density and Improvement of Urban Technological Efficiency: An Estimation Based on the Stochastic Frontier Approach
Authors: Guo Jing , Wang Ying , Xu Haidong , Li Bo , Wang Yang
Published: August 2023

Exploring the relationship between finance and economic growth is a key direction of financial economics. However, most of the literature starts from the aggregate perspective and uses the GDP or per capita GDP as the explained variable to study the role of finance. Such a perspective ignores the heterogeneity of financial activities with respect to geographical distribution and makes it difficult to distinguish the roles of factor input and efficiency improvement. Because of this, this article introduces a “density” perspective on new economic geography and the measurement of the efficiency of the transition of development economics into financial economics. This article uses the stochastic frontier analysis (SFA) method to measure the technical efficiency (TE) of 272 cities in China from 2005 to 2018 and then, based on “forward-looking” and “backward-looking” methods, measures the impact of financial density on urban technical efficiency. This study found that overall, before the financial crisis in 2008, the contribution of financial density to technical efficiency showed a downward trend, and in the regional and provincial dimensions, the distribution of financial density’s contribution to technical efficiency was generally in line with that of backward regions, with less regularity in developed regions. In the urban dimension, the contribution rate of financial density to resource-based cities with slow technological progress or advanced cities with rich financial resources is not very prominent and may even play a negative role; however, cities that are at a medium level of development, rich in population resources, have convenient transportation, and have a certain industrial foundation can greatly promote the improvement of technical efficiency. Therefore, it may be possible to optimize the marginal contribution of urban financial density to the technical efficiency of Chinese cities by encouraging the flow of financial resources and activities from cities with small marginal effects to those with large marginal effects.
Examining the Effects of Built Environments and Individual Characteristics on Commuting Time under Spatial Heterogeneity: An Empirical Study in China Using HLM
Authors: Zhang Mei , Tang Jia , Gao Jun
Published: August 2023

A large number of studies have provided evidence regarding the factors that influence commuting time. However, few studies have explored such effects in the context of considering spatial heterogeneity across cities, which limits the generalizability of the findings. This study addresses this gap by utilizing a dataset of 113 cities in China across the years 2014, 2016, 2018, and 2020. A two-level hierarchical linear model (HLM) was developed to explore the combined effects of city-level and individual-level factors on commuting time by constructing a nested “city-individual” relationship. The results show that (1) built environments at the city level significantly impact commuting time; (2) a non-linear association between population density and commuting time (U-shaped relationship) was identified, as well as between the number of buses and commuting time (inverted U-shaped relationship); (3) the urban construction land area and road area per capita exert negative effects on commuting time; (4) the impacts of individuals’ jobs–housing balance, travel allowances, and education on commuting time vary across cities. These findings might contribute to optimizing the design of a built environment, addressing the challenge posed by longer commuting times, and providing a better understanding of the effects of individuals’ characteristics on commuting time while considering the inherent differences across cities.
Multiple Probability Ecological Network and County-Scale Management
Authors: Xie Jing , Xie Binggeng , Zhou Kaichun , Li Junhan , Xiao Jianyong , Liu Changchang , Zhang Xuemao
Published: August 2023

Ecological networks are threatened by rapid urbanization; the implementation of ecological network management and maintenance strategies is essential in the county units of urban agglomerations. This study focused on the Changsha–Zhuzhou–Xiangtan urban agglomeration (CZXUA) and objectively identified and evaluated the regional ecological networks. Connectivity distance thresholds were determined using goodness-of-fit experiments. The Probability of becoming an Ecological Source (PES) was constructed to investigate the ecological networks at different probabilities, and the network weight in each county was analyzed based on the gravity model. The results show that: (1) The distance thresholds were 2500, 2700, and 2300 m in 2000, 2010, and 2020, respectively. Source degradation and corridor disconnection were most significant at a PES > 50%. (2) At a PES > 50%, the high gravitational value increased from 39,972 to 31,4642, owing to fragmentation. Fourteen counties received weights, and fluctuations were most significant at a PES > 90%. (3) The trends in the PES and gravitational value were not always synergistic, and the negative impact of source degradation on the gravitational value was significantly less than the positive impact of the reduced distance between sources. The gravity center movement under the high PES determined the ecological network status of neighboring counties. The findings can quantify ecological information flow and provide a basis for county ecological management and restoration.
Exploring the Ethical Dimensions of Using ChatGPT in Language Learning and Beyond
Authors: Vaccino-Salvadore Silvia
Published: August 2023

The emergence of ChatGPT in the field of education has opened up new opportunities for language learning, but it has also brought about significant ethical considerations that must be carefully considered and addressed to ensure that this technology is used responsibly. With the field of artificial intelligence (AI) advancing at an unprecedented rate, it is imperative for educators and administrators to remain vigilant in monitoring the ethical implications of integrating ChatGPT into language education and beyond. This paper will explore several ethical dimensions concerning the use of ChatGPT, a sophisticated language model developed by OpenAI, in language education. It will discuss privacy, bias, reliability, accessibility, authenticity, and academic integrity as significant ethical implications to consider while integrating ChatGPT into the language classroom. By gaining an initial understanding of the ethical implications involved in utilizing ChatGPT in language education, students, teachers, and administrators will be able to make informed decisions about the appropriate use of the technology, ensuring that it is employed in an ethical and responsible manner.