bluuebunny/crossref_metadata_2025 huggingface dataset:
Tetramethylpyrazine Preserves the Integrity of Blood-Brain Barrier Associated With Upregulation of MCPIP1 in a Murine Model of Focal Ischemic Stroke
Authors: Jin Zhuqing , Liang Jian , Kolattukudy Pappachan E.
Published: July 2021

Tetramethylpyrazine (TMP), a prominent ingredient of Chinese herb Ligusticum chuanxiong Hort, is known to suppress neuroinflammation and protect blood-brain barrier (BBB) integrity. We investigated whether monocyte chemotactic protein-induced protein 1 (MCPIP1, also known as Regnase-1), a newly identified zinc-finger protein, plays a role in TMP-mediated anti-inflammation and neuroprotection. Male C57BL/6 mice were subjected to focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO) for 2 h, followed by reperfusion for 24 h. TMP (25 mg/kg or 50 mg/kg) or vehicle was administered intraperitoneally 12 h before and post MCAO. The TMP significantly upregulated MCPIP1 in the ischemic brain tissues and effectively inhibited extravasation of fluorescein isothiocyanate (FITC)-dextran, resulting in attenuation of brain edema. These effects of the TMP were associated with a significant reduction in levels of inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and MMP-9 in the ischemic brain tissues. The TMP upregulated the expression of MCPIP1 in primary cultures of neurons and protected against oxygen–glucose deprivation-induced neuron death, while this neuroprotective effect of TMP was abolished by knockdown of MCPIP1 using MCPIP1-specific siRNA. These results suggest that preservation of BBB integrity by TMP is associated with its anti-inflammatory activity. The effect of TMP is mediated, at least in part, via upregulation of MCPIP1 in the ischemic brain.
Electronic Cigarette Exposure Enhances Lung Inflammatory and Fibrotic Responses in COPD Mice
Authors: Han Hongwei , Peng Guangda , Meister Maureen , Yao Hongwei , Yang Jenny J. , Zou Ming-Hui , Liu Zhi-Ren , Ji Xiangming
Published: July 2021

Although a few studies show that the use of electronic nicotine delivery systems (ENDS) may ameliorate objective and subjective outcomes in COPD smokers who switched to electronic cigarettes, it is unclear whether e-cigarette exposure alters lung pathological features and inflammatory response in COPD. Here, we employed βENaC-overexpressing mice bearing COPD-like pulmonary abnormality, and exposed them to ENDS. We found that ENDS exposure aggravated airspace enlargement and mucus production in βENaC-overexpressing mice, which was associated with increased MMP12 and Muc5ac, respectively. ENDS exposure to mice significantly increased the numbers of macrophages, particularly in M2 macrophages in bronchoalveolar lavage (BAL) fluid, despite ENDS did not induce M2 macrophage polarization in a cultured murine macrophage cell line (RAW264.7). There were no changes in neutrophils in BAL fluid by ENDS exposure. Multiple cytokine productions were increased including M-CSF, IL-1rα, IL-10, and TGF-β1, in BAL fluid from mice when exposed to ENDS. The Sirius Red staining and hydroxyproline assay showed ENDS-exposed mice displayed enhanced fibrotic phenotypes compared to control mice. In conclusion, ENDS exposure enhances airspace enlargement, mucus secretion, and fibrogenesis in COPD mice. This is associated with increased MMP12, inflammatory responses, and M2 macrophage phenotype. This study provides pre-clinical data implicating that electronic cigarette exposure is not safe in COPD patients who want to replace traditional cigarettes with ENDS.
Adaptive Capacities and Complexity of Heart Rate Variability in Patients With Chronic Obstructive Pulmonary Disease Throughout Pulmonary Rehabilitation
Authors: Hognon Louis , Heraud Nelly , Varray Alain , Torre Kjerstin
Published: July 2021

Introduction

The complexity of bio-signals, like R-R intervals, is considered a reflection of the organism’s capacity to adapt. However, this association still remains to be consolidated. We investigated whether the complexity of R-R intervals at rest and during perturbation [6-minute walking test (6MWT)], yielded information regarding adaptive capacities in Chronic Obstructive Pulmonary Disease (COPD) patients during pulmonary rehabilitation (PR).

Methods

In total, 23 COPD patients (64 ± 8 years, with forced expiratory volume in 1 s of 55 ± 19% predicted) were tested three times at the start (T1), middle (T2), and end (T3) of 4 weeks PR. Each time, R-R intervals were measured at rest and during 6MWT. The complexity of R-R intervals was assessed by evenly spaced Detrended Fluctuations Analysis and evaluated by the fractal exponent α and deviation from maximal complexity |1-α|.

Results

The 6MWT distance was significantly increased at T2 and T3 compared to T1. Neither α nor |1-α| at rest and during perturbation significantly changed throughout PR, nor were they consistently associated with 6MWT distances at each time. Throughout the PR program, complexity during the 6MWT was significantly lower compared to the rest. The level of α during 6MWT at T1 was positively correlated with the improvement of the 6MWT distance throughout the PR program.

Discussion

Reduced complexity in COPD patients during acute perturbation at the beginning of PR supports a decreased improvement of the 6MWT distance throughout PR. This result seems consistent with the notion that the complexity reflects the patients’ adaptive capacities and could therefore become a clinical indicator in an applied perspective.
Effects of Lung Injury on Regional Aeration and Expiratory Time Constants: Insights From Four-Dimensional Computed Tomography Image Registration
Authors: Herrmann Jacob , Gerard Sarah E. , Shao Wei , Xin Yi , Cereda Maurizio , Reinhardt Joseph M. , Christensen Gary E. , Hoffman Eric A. , Kaczka David W.
Published: July 2021

Rationale: Intratidal changes in regional lung aeration, as assessed with dynamic four-dimensional computed tomography (CT; 4DCT), may indicate the processes of recruitment and derecruitment, thus portending atelectrauma during mechanical ventilation. In this study, we characterized the time constants associated with deaeration during the expiratory phase of pressure-controlled ventilation in pigs before and after acute lung injury using respiratory-gated 4DCT and image registration.

Methods: Eleven pigs were mechanically ventilated in pressure-controlled mode under baseline conditions and following an oleic acid model of acute lung injury. Dynamic 4DCT scans were acquired without interrupting ventilation. Automated segmentation of lung parenchyma was obtained by a convolutional neural network. Respiratory structures were aligned using 4D image registration. Exponential regression was performed on the time-varying CT density in each aligned voxel during exhalation, resulting in regional estimates of intratidal aeration change and deaeration time constants. Regressions were also performed for regional and total exhaled gas volume changes.

Results: Normally and poorly aerated lung regions demonstrated the largest median intratidal aeration changes during exhalation, compared to minimal changes within hyper- and non-aerated regions. Following lung injury, median time constants throughout normally aerated regions within each subject were greater than respective values for poorly aerated regions. However, parametric response mapping revealed an association between larger intratidal aeration changes and slower time constants. Lower aeration and faster time constants were observed for the dependent lung regions in the supine position. Regional gas volume changes exhibited faster time constants compared to regional density time constants, as well as better correspondence to total exhaled volume time constants.

Conclusion: Mechanical time constants based on exhaled gas volume underestimate regional aeration time constants. After lung injury, poorly aerated regions experience larger intratidal changes in aeration over shorter time scales compared to normally aerated regions. However, the largest intratidal aeration changes occur over the longest time scales within poorly aerated regions. These dynamic 4DCT imaging data provide supporting evidence for the susceptibility of poorly aerated regions to ventilator-induced lung injury, and for the functional benefits of short exhalation times during mechanical ventilation of injured lungs.
Altered Vascular Adaptations to Pregnancy in a Rat Model of Advanced Maternal Age
Authors: Pasha Mazhar , Wooldridge Amy L. , Kirschenman Raven , Spaans Floor , Davidge Sandra T. , Cooke Christy-Lynn M.
Published: July 2021

Advanced maternal age (≥35 years old) increases the risk of pregnancy complications such as preeclampsia and fetal growth restriction. We previously demonstrated vascular dysfunction and abnormal pregnancy outcomes in a rat model of advanced maternal age. However, vascular adaptations to pregnancy in aging were not studied. We hypothesize that advanced maternal age is associated with a more vasoconstrictive phenotype due to reduced nitric oxide (NO) and increased activity of matrix metalloproteinases (MMPs), contributing to impaired vascular adaptations to pregnancy. A rat model of advanced maternal age was used: young (4 months) and aged (9.5 months; ∼35 years in humans) non-pregnant and pregnant rats. On gestational day 20 (term = 22 days; non-pregnant rats were aged-matched), blood pressure and heart rate were measured (tail cuff plethysmography) and vascular function was assessed in mesenteric arteries (wire myography). Endothelium-dependent relaxation to methylcholine (MCh) was assessed in the presence/absence of nitric oxide synthase inhibitor (L-NAME), or inhibitors of endothelium-dependent hyperpolarization (EDH; apamin and TRAM-34). Vasoconstriction responses to big endothelin-1 (bigET-1), in the presence/absence of MMPs-inhibitor (GM6001) or endothelin converting enzyme (ECE-1) inhibitor (CGS35066), in addition, ET-1 responsiveness, were measured. Blood pressure was elevated only in aged non-pregnant rats (p< 0.001) compared to all other groups. MCh responses were not different, however, L-NAME decreased maximum vasodilation in young (p< 0.01) and aged pregnant rats (p< 0.001), and decreased MCh sensitivity in young non-pregnant rats (p< 0.01), without effects in aged non-pregnant rats. EDH contribution to relaxation was similar in young non-pregnant, and aged non-pregnant and pregnant rats, while EDH-mediated relaxation was absent in young pregnant rats (p< 0.001). BigET-1 responses were enhanced in aged non-pregnant (p< 0.01) and pregnant rats (p< 0.05). No significant changes in bigET-1 conversion occurred in the presence of MMP-inhibitor, whereas ECE-1 inhibition reduced bigET-1 constriction in aged rats (p< 0.01). No differences in ET-1 sensitivity were observed. In conclusion, contrary to our hypothesis, reduced blood pressure, and an increased EDH-dependent contribution to vasodilation suggest a compensatory mechanism that may reflect beneficial adaptations in these aged rats that were able to maintain pregnancy. These data increase our understanding of how the vascular adaptive pathways in pregnancy compensate for advanced maternal age.
PPR-DYW Protein EMP17 Is Required for Mitochondrial RNA Editing, Complex III Biogenesis, and Seed Development in Maize
Authors: Wang Yong , Liu Xin-Yuan , Huang Zi-Qin , Li Yan-Yan , Yang Yan-Zhuo , Sayyed Aqib , Sun Feng , Gu Zhi-Qun , Wang Xiaomin , Tan Bao-Cai
Published: July 2021

The conversion of cytidines to uridines (C-to-U) at specific sites in mitochondrial and plastid transcripts is a post-transcriptional processing event that is important to the expression of organellar genes. Pentatricopeptide repeat (PPR) proteins are involved in this process. In this study, we report the function of a previously uncharacterized PPR-DYW protein, Empty pericarp17 (EMP17), in the C-to-U editing and kernel development in maize. EMP17 is targeted to mitochondria. The loss-function of EMP17 arrests maize kernel development, abolishes the editing at ccmFC-799 and nad2-677 sites, and reduces the editing at ccmFC-906 and -966 sites. The absence of editing causes amino acid residue changes in CcmFC-267 (Ser to Pro) and Nad2-226 (Phe to Ser), respectively. As CcmFC functions in cytochrome c (Cytc) maturation, the amount of Cytc and Cytc1 protein is drastically reduced in emp17, suggesting that the CcmFC-267 (Ser to Pro) change impairs the CcmFC function. As a result, the assembly of complex III is strikingly decreased in emp17. In contrast, the assembly of complex I appears less affected, suggesting that the Nad2-226 (Phe to Ser) change may have less impact on Nad2 function. Together, these results indicate that EMP17 is required for the C-to-U editing at several sites in mitochondrial transcripts, complex III biogenesis, and seed development in maize.
Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction
Authors: Hug Sebastian , Liu Yilei , Heiniger Benjamin , Bailly Aurélien , Ahrens Christian H. , Eberl Leo , Pessi Gabriella
Published: July 2021

Paraburkholderia phymatum STM815, a rhizobial strain of the Burkholderiaceae family, is able to nodulate a broad range of legumes including the agriculturally important Phaseolus vulgaris (common bean). P. phymatum harbors two type VI Secretion Systems (T6SS-b and T6SS-3) in its genome that contribute to its high interbacterial competitiveness in vitro and in infecting the roots of several legumes. In this study, we show that P. phymatum T6SS-b is found in the genomes of several soil-dwelling plant symbionts and that its expression is induced by the presence of citrate and is higher at 20/28°C compared to 37°C. Conversely, T6SS-3 shows homologies to T6SS clusters found in several pathogenic Burkholderia strains, is more prominently expressed with succinate during stationary phase and at 37°C. In addition, T6SS-b expression was activated in the presence of germinated seeds as well as in P. vulgaris and Mimosa pudica root nodules. Phenotypic analysis of selected deletion mutant strains suggested a role of T6SS-b in motility but not at later stages of the interaction with legumes. In contrast, the T6SS-3 mutant was not affected in any of the free-living and symbiotic phenotypes examined. Thus, P. phymatum T6SS-b is potentially important for the early infection step in the symbiosis with legumes.
Melatonin Relieves Ozone Stress in Grape Leaves by Inhibiting Ethylene Biosynthesis
Authors: Liu Chuang , Kang Hui , Wang Yafang , Yao Yuxin , Gao Zhen , Du Yuanpeng
Published: July 2021

Ozone (O3) stress severely affects the normal growth of grape (Vitis vinifera L.) leaves. Melatonin (MT) plays a significant role in plant response to various abiotic stresses, but its role in O3 stress and related mechanisms are poorly understood. In order to understand the mechanism of MT in alleviate O3 stress in grape leaves, we perform a transcriptome analyses of grapes leaves under O3 stress with or without MT treatment. Transcriptome analysis showed that the processes of ethylene biosynthesis and signaling were clearly changed in “Cabernet Sauvignon” grapes under O3 and MT treatment. O3 stress induced the expression of genes related to ethylene biosynthesis and signal transduction, while MT treatment significantly inhibited the ethylene response mediated by O3 stress. Further experiments showed that both MT and aminoethoxyvinylglycine (AVG, an inhibitor of ethylene biosynthesis) enhanced the photosynthetic and antioxidant capacities of grape leaves under O3 stress, while ethephon inhibited those capacities. The combined treatment effect of MT and ethylene inhibitor was similar to that of MT alone. Exogenous MT reduced ethylene production in grape leaves under O3 stress, while ethephon and ethylene inhibitors had little effect on the MT content of grape leaves after O3 stress. However, overexpression of VvACO2 (1-aminocyclopropane-1-carboxylate oxidase2) in grape leaves endogenously induced ethylene accumulation and aggravated O3 stress. Overexpression of the MT synthesis gene VvASMT1 (acetylserotonin methyltransferase1) in tobacco (Nicotiana tabacum L.) alleviated O3 stress and reduced ethylene biosynthesis after O3 stress. In summary, MT can alleviate O3 stress in grape leaves by inhibiting ethylene biosynthesis.
Respiration Interacts With Photosynthesis Through the Acceptor Side of Photosystem I, Reflected in the Dark-to-Light Induction Kinetics of Chlorophyll Fluorescence in the Cyanobacterium Synechocystis sp. PCC 6803
Authors: Ogawa Takako , Suzuki Kenta , Sonoike Kintake
Published: July 2021

In cyanobacteria, the photosynthetic prokaryotes, direct interaction between photosynthesis and respiration exists at plastoquinone (PQ) pool, which is shared by the two electron transport chains. Another possible point of intersection of the two electron transport chains is NADPH, which is the major electron donor to the respiratory chain as well as the final product of the photosynthetic chain. Here, we showed that the redox state of NADPH in the dark affected chlorophyll fluorescence induction in the cyanobacterium Synechocystis sp. PCC 6803 in a quantitative manner. Accumulation of the reduced NADPH in the dark due to the defect in type 1 NAD(P)H dehydrogenase complex in the respiratory chain resulted in the faster rise to the peak in the dark-to-light induction of chlorophyll fluorescence, while depletion of NADPH due to the defect in pentose phosphate pathway resulted in the delayed appearance of the initial peak in the induction kinetics. There was a strong correlation between the dark level of NADPH determined by its fluorescence and the peak position of the induction kinetics of chlorophyll fluorescence. These results indicate that photosynthesis interacts with respiration through NADPH, which enable us to monitor the redox condition of the acceptor side of photosystem I by simple measurements of chlorophyll fluorescence induction in cyanobacteria.
Nucleocytoplasmic Communication in Healthy and Diseased Plant Tissues
Authors: Lüdke Daniel , Rohmann Philipp F. W. , Wiermer Marcel
Published: July 2021

The double membrane of the nuclear envelope (NE) constitutes a selective compartment barrier that separates nuclear from cytoplasmic processes. Plant viability and responses to a changing environment depend on the spatial communication between both compartments. This communication is based on the bidirectional exchange of proteins and RNAs and is regulated by a sophisticated transport machinery. Macromolecular traffic across the NE depends on nuclear transport receptors (NTRs) that mediate nuclear import (i.e. importins) or export (i.e. exportins), as well as on nuclear pore complexes (NPCs) that are composed of nucleoporin proteins (NUPs) and span the NE. In this review, we provide an overview of plant NPC- and NTR-directed cargo transport and we consider transport independent functions of NPCs and NE-associated proteins in regulating plant developmental processes and responses to environmental stresses.
The Attitude of Work-Oriented and Family-Oriented Chinese Women Toward the Evaluations Based on the Traditional Positive Stereotype That Women Are Virtuous
Authors: Song Jingjing , Li Junnan , Liu Yanfen , Ruan Yifan
Published: July 2021

People typically reject being negatively stereotyped but overlook the ways in which they are positively stereotyped. The current study focused on the attitude of Chinese women toward being evaluated based on the traditional positive stereotype that women are virtuous; family/work centrality as a boundary condition of these attitudes; and three perceptions that may mediate the link between this type of evaluation and attitudes of women. In experiment 1, female college students were identified as work-oriented or family-oriented based on their responses to a questionnaire regarding their focus on these two domains. They then read a vignette in which a man evaluated a female target under random assignment to one of three conditions, namely: group positive stereotype evaluation, individual positive stereotype evaluation, or unstereotypical positive evaluation. The participants rated how much they liked the female target, as an indicator of their attitude toward evaluations based on the stereotype that women are virtuous. In experiment 2, female college students were classified as work- oriented or family-oriented, and then read a vignette in which a man (the target) evaluated them. They were randomly assigned to the group positive stereotype evaluation, individual positive stereotype evaluation, or unstereotypical positive evaluation. Participants rated how much they liked the male target, as an indicator of their attitude toward evaluations based on the positive stereotype that women are virtuous. Across both studies, ANOVA showed that work-oriented women liked evaluations based on both group and individual stereotypes less than the family-oriented women. Regression-based analyses showed evidence of a mediation process in which work-oriented women viewed the virtuous positive stereotype as implying a prescriptive social demand that women should engage in family roles, resulting in a negative reaction to this type of evaluation.
Emotional Granularity Increases With Intensive Ambulatory Assessment: Methodological and Individual Factors Influence How Much
Authors: Hoemann Katie , Barrett Lisa Feldman , Quigley Karen S.
Published: July 2021

Individuals differ in their ability to create instances of emotion that are precise and context-specific. This skill – referred to as emotional granularity or emotion differentiation – is associated with positive mental health outcomes. To date, however, little work has examined whether and how emotional granularity might be increased. Emotional granularity is typically measured using data from experience sampling studies, in which participants are prompted to report on their emotional experiences multiple times per day, across multiple days. This measurement approach allows researchers to examine patterns of responses over time using real-world events. Recent work suggests that experience sampling itself may facilitate increases in emotional granularity in depressed individuals, such that it may serve both empirical and interventional functions. We replicated and extended these findings in healthy adults, using data from an intensive ambulatory assessment study including experience sampling, peripheral physiological monitoring, and end-of-day diaries. We also identified factors that might distinguish individuals who showed larger increases over the course of experience sampling and examined the extent of the impact of these factors. We found that increases in emotional granularity over time were facilitated by methodological factors, such as number of experience sampling prompts responded to per day, as well as individual factors, such as resting respiratory sinus arrhythmia. These results provide support for the use of experience sampling methods to improve emotional granularity, raise questions about the boundary conditions of this effect, and have implications for the conceptualization of emotional granularity and its relationship with emotional health.
Physical and Psychoacoustic Characteristics of Typical Noise on Construction Site: “How Does Noise Impact Construction Workers’ Experience?”
Authors: Yang Xinhao , Wang Yitong , Zhang Ruining , Zhang Yuan
Published: July 2021

Construction noise is an integral part of urban social noise. Construction workers are more directly and significantly affected by construction noise. Therefore, the construction noise situation within construction sites, the acoustic environment experience of construction workers, and the impact of noise on them are highly worthy of attention. This research conducted a 7-month noise level (LAeq) measurement on a construction site of a reinforced concrete structure high-rise residential building in northern China. The noise conditions within the site in different spatial areas and temporal stages was analyzed. Binaural recording of 10 typical construction noises, including earthwork machinery, concrete machinery, and hand-held machinery, were performed. The physical acoustics and psychoacoustic characteristics were analyzed with the aid of a sound quality analysis software. A total of 133 construction workers performing 12 types of tasks were asked about their subjective evaluation of the typical noises and given a survey on their noise experience on the construction site. This was done to explore the acoustic environment on the construction site, the environmental experience of construction workers, the impact of noise on hearing and on-site communications, and the corresponding influencing factors. This research showed that the noise situation on construction sites is not optimistic, and the construction workers have been affected to varying degrees in terms of psychological experience, hearing ability, and on-site communications. Partial correlation analysis showed that the construction workers’ perception of noise, their hearing, and their on-site communications were affected by the noise environment, which were correlated to varying degrees with the individual’s post-specific noise, demand for on-site communications, and age, respectively. Correlation analysis and cluster analysis both showed that the annoyance caused by typical construction noise was correlated to its physical and psychoacoustic characteristics. To maintain the physical and mental health of construction workers, there is a need to improve on the fronts of site management, noise reduction, equipment and facility optimization, and occupational protection.
Research on Emotion Analysis of Chinese Literati Painting Images Based on Deep Learning
Authors: Zhang Jie , Duan Yingjing , Gu Xiaoqing
Published: July 2021

Starting from a pure-image perspective, using machine learning in emotion analysis methods to study artwork is a new cross-cutting approach in the field of literati painting and is an effective supplement to research conducted from the perspectives of aesthetics, philosophy, and history. This study constructed a literati painting emotion dataset. Five classic deep learning models were used to test the dataset and select the most suitable model, which was then improved upon for literati painting emotion analysis based on accuracy and model characteristics. The final training accuracy rate of the improved model was 54.17%. This process visualizes the salient feature areas of the picture in machine vision, analyzes the visualization results, and summarizes the connection law between the picture content of the Chinese literati painting and the emotion expressed by the painter. This study validates the possibility of combining deep learning with Chinese cultural research, provides new ideas for the combination of new technology and traditional Chinese literati painting research, and provides a better understanding of the Chinese cultural spirit and advanced factors.
Imaging Brain Glx Dynamics in Response to Pressure Pain Stimulation: A 1H-fMRS Study
Authors: Jelen Luke A. , Lythgoe David J. , Jackson Jade B. , Howard Matthew A. , Stone James M. , Egerton Alice
Published: July 2021

Glutamate signalling is increasingly implicated across a range of psychiatric, neurological and pain disorders. Reliable methodologies are needed to probe the glutamate system and understand glutamate dynamics in vivo. Functional magnetic resonance spectroscopy (1H-fMRS) is a technique that allows measurement of glutamatergic metabolites over time in response to task conditions including painful stimuli. In this study, 18 healthy volunteers underwent 1H-fMRS during a pressure-pain paradigm (8 blocks of REST and 8 blocks of PAIN) across two separate sessions. During each session, estimates of glutamate + glutamine (Glx), scaled to total creatine (tCr = creatine + phosphocreatine) were determined for averaged REST and PAIN conditions within two separate regions of interest: the anterior cingulate cortex (ACC) and dorsal ACC (dACC). A two-way repeated measures analysis of variance determined a significant main effect of CONDITION (p = 0.025), with higher Glx/tCr during PAIN compared to REST across combined sessions, in the dACC ROI only. However, increases in dACC Glx/tCr during PAIN compared to REST showed limited reliability and reproducibility across sessions. Future test-retest 1H-fMRS studies should examine modified or alternative paradigms to determine more reliable methodologies to challenge the glutamate system that may then be applied in patient groups and experimental medicine studies.
Anxiety and Depression in Patients With Physical Diseases and Associated Factors: A Large-Scale Field Survey in General Hospitals in China
Authors: Yang Wanlin , Xiao Ling , Yuan Zhiyong , Huang Huan , Xiang Yilei , Liu Zhongchun , Nan Cai , Wang Huiling , Wang Gaohua
Published: July 2021

Introduction: To investigate the characteristic of anxiety and depression among patients in general hospitals, and explore the degree of the clinical symptoms and correlated social economic factors.

Methods: This is a cross-sectional survey of anxiety and depression in patients with physical diseases, who were suspected of depression and anxiety based on their clinical performance by their physicians and PHQ ≧ 8, from various clinical departments of 57 general hospitals in China. Data regarding demographic characteristics and clinical characteristics were collected. Social and psychological factors and the severity of anxiety or depression were collected through self-rating scales. Finally, we used multivariate logistic regression to identify the factors associated with anxiety and depression in patients with physical diseases.

Results: A total of 2,105 (84.6%) valid and completed questionnaires were returned. The proportion of anxiety, depression, combined depression and anxiety, either anxiety or depression among the patients with physical diseases from all clinical departments was 63.3, 75.1, 57.1, and 81.2% respectively. Further regression analysis indicated that gender, monthly income, specific physical diseases, personality traits, social supports and life negative events were related factors of both anxiety and depression.

Conclusions: Anxiety and depression were common in patients with physical diseases, with a high proportion of co-morbidity of anxiety and depression. Females, patients with cancer, poor social support and negative life events reported more severe anxiety and depression. The results may help to understand the present situation of anxiety and depression in general hospitals in china, and identify the patients with high risk of depression and anxiety.
Similar or Different Neuropsychological Profiles? Only Set Shifting Differentiates Women With Bipolar vs. Borderline Personality Disorder
Authors: Michopoulos Ioannis , Tournikioti Kalliopi , Paraschakis Antonios , Karavia Anna , Gournellis Rossetos , Smyrnis Nikolaos , Ferentinos Panagiotis
Published: July 2021

There is ongoing debate about the similarities and differences between bipolar disorder (BD) and borderline personality disorder (BPD). Very few studies have concurrently assessed their neuropsychological profile and only on a narrow array of neuropsychological tests. We aimed to investigate the differences of these two patient groups on visual memory, executive function, and response inhibition. Twenty-nine BD patients, 27 BPD patients and 22 controls (all female) were directly compared on paired associates learning (PAL), set shifting (ID/ED), problem solving (SOC), and response inhibition (SSRT) using Cambridge Neuropsychological Test Automated Battery (CANTAB). Rank-normalized outcomes were contrasted in one-way ANOVA tests. Discriminant analysis was finally performed to predict BD or BPD patient status. BD patients performed significantly worse than controls on all tasks. BPD patients performed significantly worse than HC on all tests except SST. Significant differences between the two patient groups were recorded only on ID/ED, where BPD patients performed worse (p = 0.044). A forward stepwise discriminant analysis model based on ID/ED and SOC predicted correctly patients' group at 67.9% of cases. In conclusion, BD and BPD female patients appear to be more similar than different as regards their neuropsychological functions. This study is the first to show that BPD patients display more deficits than BD patients when directly compared on the set shifting executive function test, a marker of cognitive flexibility. Discerning BD from BPD patients through neuropsychological performance is promising but would improve by using additional subtler tests and psychometric evaluation.
What Is Our Most Important Sense?
Authors: Hutmacher Fabian
Published: July 2021

For most people, vision is their most important sense. Indeed, vision is crucial in many situations of our daily lives—like reading this article. Additionally, a large part of the human brain is dedicated to processing visual information, while information from the other senses is processed in much smaller brain regions. However, we should not forget about the importance of our other senses too quickly. Each sense provides us with unique information about ourselves and the world. In other words, each sense captures a different aspect of reality. If we understand this, we can start to appreciate the richness and diversity of our sensory abilities in a new way. We can be grateful that we have all of our senses and that they give us such a diverse access to the world.
Elevated [CO2] Mitigates Drought Effects and Increases Leaf 5-O-Caffeoylquinic Acid and Caffeine Concentrations During the Early Growth of Coffea Arabica Plants
Authors: Catarino Ingrid C. A. , Monteiro Gustavo B. , Ferreira Marcelo J. P. , Torres Luce M. B. , Domingues Douglas S. , Centeno Danilo C. , Lobo Ana Karla M. , Silva Emerson A.
Published: July 2021

Increasing atmospheric [CO2] is thought to contribute to changes in precipitation patterns, increasing heatwaves and severe drought scenarios. However, how the combination of elevated [CO2] and progressive drought affect plant metabolism is poorly understood. Aiming to investigate the effects of this environmental condition on photosynthesis and specialized metabolites in leaves ofCoffea arabicaduring the early growth, plants fertilized with ambient (a[CO2]-400 ppm) and elevated (e[CO2]-800 ppm) [CO2] were exposed to well-watered (WW) or water-deficit (WD) regimes for 40 days. Over the 40-day-water-withdrawal, soil moisture, and leaf water potential decreased compared to WW-condition. Elevated [CO2] stimulates CO2assimilation (A) and intrinsic water use efficiency (iWUE) even under WD. Drought condition slightly changed stomatal conductance, transpiration rate and maximum quantum efficiency of photosystem II (PSII) regardless of [CO2] compared to WW-plants. Total soluble amino acid concentration did not change significantly, while total phenolic compounds concentration decreased under e[CO2] regardless of water regimes. The combination of e[CO2]+WD increased the 5-O-caffeoylquinic acid (5-CQA) and caffeine amounts by 40-day when compared to a[CO2]+WD plants. Altogether, these results suggest that e[CO2] buffers mild-drought stress in youngC. arabicaby increasing A, iWUE and stimulating changes in the leaf contents of 5-CQA and caffeine.
Variations in the Density and Distribution of Cajal Like Cells Associated With the Pathogenesis of Ureteropelvic Junction Obstruction: A Systematic Review and Meta-Analysis
Authors: Samaranayake U. M. J. E. , Mathangasinghe Y. , Liyanage U. A. , de Silva M. V. C. , Samarasinghe M. C. , Abeygunasekera S. , Lamahewage A. K. , Malalasekera A. P.
Published: July 2021

Introduction: Cajal like cells (CLCs) in the upper urinary tract have an ability to generate coordinated spontaneous action potentials and are hypothesized to help propel urine from renal pelvis into the ureter. The objective of this review was to describe the variations in the density and distribution of CLCs associated with ureteropelvic junction obstruction (UPJO).

Materials and Methods: Studies comparing the density and distribution of CLCs in the human upper urinary tract in patients with UPJO and healthy controls were included in this systematic review. We searched online electronic databases; Ovid MEDLINE, Scopus, PubMed and Cochrane reviews for the studies published before October 31, 2020. A meta-analysis was conducted to compare the density of CLCs at the ureteropelvic junction (UPJ) in patients with UPJO and matched controls.

Results: We included 20 and seven studies in the qualitative and quantitative synthesis, respectively. In majority (55%) CLCs were located between the muscle layers of the upper urinary tract. The CLC density in the UPJ gradually increased with aging in both healthy subjects and patients with UPJO. The pooled analysis revealed that the density of CLCs at the UPJ was significantly low in patients with UPJO compared to the controls (SMD = −3.00, 95% CI = −3.89 to −2.11, p < 0.01).

Conclusions: The reduction in CLC density at the UPJ in patients with UPJO suggests a contribution from CLCs in the pathogenesis of UPJO. Since age positively correlates with CLC density, it is imperative to carefully match age when conducting case control studies comparing the CLC density and distribution.

Protocol Registration Number: CRD42020219882.
Probing the Links between Workforce Diversity, Goal Clarity, and Employee Job Satisfaction in Public Sector Organizations
Authors: Stazyk Edmund C. , Davis Randall S. , Liang Jiaqi
Published: July 2021

A considerable body of research substantiating the importance of workforce diversity to public organizations has accrued over the past two decades. However, research on workforce diversity has also been narrow in scope and frequently fails to link diversity to important individual and organizational outcomes. Using data (n = 1,109,134 employees from 500 sub-agencies) collected in three waves (2010, 2011, and 2012) of the Federal Employee Viewpoint Survey (FEVS), this study examines whether (1) increased diversity influences organizational goal clarity, (2) diversity and goal clarity, in turn, influence employee job satisfaction, and (3) diversity management policies influence job satisfaction by clarifying organizational goals for workers. FEVS is administered yearly by the U.S. Office of Personnel Management (OPM) and is designed to assess whether and to what extent federal employees believe the characteristics of successful organizations are present in their agency. Results from a multi-level structural equation model (MSEM) suggest diversity is associated with greater goal clarity and that diversity management policies, by clarifying organizational goals, positively affect job satisfaction. Findings also indicate that the type of diversity matters.
Productivity and Reproductive Performance of Mixed-Age Ewes across 20 Years of Selection for Ultrafine Wool in Uruguay
Authors: Ramos Zully , Blair Hugh Thomas , De Barbieri Ignacio , Ciappesoni Gabriel , Montossi Fabio , Kenyon Paul Richard
Published: July 2021

This study reports the phenotypic trends for wool, growth, and reproductive traits in mixed-age ewes after twenty years of genetic selection. Data were obtained from the Merino nucleus flock in Uruguay between 1999 and 2020. Overall, the aim of this selection flock was to reduce the fiber diameter (FD) and increase both the clean fleece weight (CFW) and live weight (LW). Data on ewe wool traits, LW, body condition score (BCS), the total number of lambs weaned (TLW), and the total LW of lambs weaned (TWW) across all lambing opportunities (1–8 mating seasons) were analyzed. Between 1292 and 2063 ewes were measured, depending on the trait considered. Ewe FD decreased by approximately 3 µm (19–16 µm), whereas greasy fleece weight (GFW) increased by 0.2 kg. This improvement in wool traits was accompanied by increases in LW at mating (3 kg), decreases in BCS at mating (approximately by 1 unit), and a small positive change in TWW across years. This study demonstrated that increasing farmer income by selecting for finer wool, heavier fleeces, and heavier animals can be obtained without compromising ewe lifetime reproduction.
Accumulation of Potentially Toxic Metals in Egyptian Alluvial Soils, Berseem Clover (Trifolium alexandrinum L.), and Groundwater after Long-Term Wastewater Irrigation
Authors: Abuzaid Ahmed S. , Jahin Hossam S. , Asaad Amany A. , Fadl Mohamed E. , AbdelRahman Mohamed A. E. , Scopa Antonio
Published: July 2021

The reduced availability of water resources in Egypt has imposed the need to intensify the use of wastewater for crop irrigation in the alluvial soils. Relevant effects can derive from contents of potentially toxic metals (PTMs) in supply resources soils, crops, and groundwater in these areas. For this reason the PTM content has to be monitored to evaluate and minimize health hazards. Therefore, in this context, two areas of the SE Nile Delta subjected to 25 year of wastewater irrigation, using agricultural drainage water (ADW) and mixed wastewater (MWW) were chosen and compared with a nearby site irrigated with Nile freshwater (NFW). At each of the three sites, ten samples of irrigation water, topsoil, berseem clover (Trifolium alexandrinum L.) plants, and seven groundwater samples were collected and analyzed for Cr, Co, Cu, Pb, Ni, and Zn. Results indicate that the total contents of Co, Cu, Ni, and Zn in soils collected from the three sampling sites and Pb in the MWW-irrigated soils were higher than their average natural contents in the earth’s crust, indicating potential risks. The DTPA-extractable contents of Cu in the three sites, in addition to Pb and Zn in the MWW-irrigated soils, exceeded the safe limits. The MWW-irrigated soils showed a considerable degree of metal contamination, while the NFW- and ADW-irrigated soils showed moderate and low levels of contamination, respectively. The contents of the six PTMs in the three sites showed low individual ecological risks, except for Pb in the MWW-irrigated soils that showed a moderate risk; however, the overall ecological risk remained low in all samples. The values of Co, Cu, and Ni in berseem shoot in addition to Pb from the MWW-irrigated soils were over the maximum permissible levels for animal feeding. Values of root-to-shoot translocation factor were lower than 1.0 for Cr, Co and Ni but higher than 1.0 for Cu, Pb, and Zn. Berssem plant is a good candidate for phytofiltration of Cr, Co and Ni, while for extracting Cu, Pb and Zn from polluted soils. The groundwater samples collected from the three sampling sites showed lower metal concentrations than the safe limits for drinking standards. Further remediation studies should be taken into account to alleviate potential environmental and health-related risks when using supply resources different from freshwater.
Effects of Different Tillage Intensities on Physicochemical and Microbial Properties of a Eutric Fluvisol Soil
Authors: Gajda Anna Maria , Czyż Ewa Antonina , Klimkowicz-Pawlas Agnieszka
Published: July 2021

The physicochemical and microbial properties of soil under long-term monoculture of winter wheat were studied to assess the effects of two tillage systems of different intensities: reduced (RT) and conventional (CT). The research was carried out on an 18-year-old experimental field at Grabów (eastern Poland) between 2018 and 2020. The RT (ploughless) and the CT (mouldboard ploughing) systems with machine operating depths of up to 10 and 25 cm, respectively, were used. The analysed parameters were as follows: soil texture, pH, readily dispersible clay content (RDC), soil organic matter (SOM), carbon from particulate organic matter (POM-C), hot- and cold-water-extractable organic carbon (HWEC, CWEC) and nitrogen (HWEN, CWEN), soil basal respiration (SBR), microbial biomass carbon (MBC) and nitrogen (MBN), nitrification potential (NP), dehydrogenases (DEH), and acid (ACP) and alkaline (ALP) phosphatases activities. Several single soil quality indices, including: metabolic (qCO2) and microbial (MicQ) quotients, enzymatic pH level indicator (EpHI), stratification ratio (SR), and metabolic potential index (MP) were calculated. The use of RT resulted in increased SOM and, therefore, in decreased RDC and increased values of soil stability, POM-C, HWEC, CWEC, HWEN, CWEN, MBC, and MBN in relation to CT. The MicQ, EpHI, SR, and MP well reflected the effects of RT and CT systems on soil and appeared to be useful in soil quality assessment. The results showed the beneficial effects on soil of the less intensive RT system in comparison with CT. Statistical analysis showed the significance of differences between tillage systems and interrelationships between the studied soil quality parameters.
Evaluation of the Quality of Irrigation Machinery by Monitoring Changes in the Coefficients of Uniformity and Non-Uniformity of Irrigation
Authors: Jobbágy Ján , Dančanin Peter , Krištof Koloman , Maga Juraj , Slaný Vlastimil
Published: July 2021

Recently, the development of agricultural technology has been focused on achieving higher reliability and quality of work. The aim of the presented paper was to examine the possibilities of evaluating the quality of work of wide-area irrigation machinery by monitoring the coefficients of uniformity and non-uniformity of irrigation. The object of the research was pivot irrigation machinery equipped with sprinklers with a total length from 230 to 540 m. The commonly applied quality of work parameter for wide-range irrigators is the irrigation uniformity coefficient according to Heermann and Hein CUH. Work quality evaluations were also carried out through other parameters applicable in practice, such as irrigation uniformity coefficients calculated according to Christiansen CU, Wilcox and Swailes Cws, and our introduced parameters, the coefficient ar (derived from the degree of unevenness according to Oehler) and the degree of uniformity γr (derived from the degree of non-uniformity according to Voight). Other applied parameters for determining the quality of work of wide-range irrigation machinery were the coefficients of irrigation uniformity according to Hart and Reynolds CUhr, further according to Criddle CUcr and subsequently according to Beale and Howell CUbr. Next, the parameters of the non-uniformity coefficient according to Oehler a, the coefficient of variation according to Stefanelli Cv, the degree of non-uniformity according to Voigt γ and the degree of non-uniformity according to Hofmeister Ef were evaluated. Field tests were performed during the growing season of cultivated crops (potatoes, corn and sugar beet) in the village of Trakovice (agricultural enterprise SLOV-MART, southwest of the Slovakia) and in the district of Piešťany (Agrobiop, joint stock company). During the research, the inlet operating parameters (speed stage, inlet pressure, irrigation dose), technical parameters (number of sprayers, total length, number of chassis) and weather conditions (wind speed and temperature) were recorded. The obtained results were examined by one-way ANOVA analysis depending on the observed coefficient or input conditions and subsequently verified by Tukey and Duncan tests as needed. Irrigation uniformity values ranged from 67.58% (Cws) to 95.88% (CUbh) depending on the input conditions. Irrigation non-uniformity values ranged from 8.58 (a, Ef) to 32.42% (Cv). The results indicate a statistically significant effect of the site of interest and thus the impact of particular field conditions (p < 0.05). When evaluating the application of different coefficients of irrigation uniformity, the results showed a statistically significant effect only in the first test (p = 0.03, p < 0.05). During further repeated measurements, the quality of work increased due to the performed inspection of all sprayers and the reduction in the influence of the wind.