bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptional mutagenesis of α-synuclein caused by DNA oxidation in Parkinson’s disease pathogenesis
Authors: Basu Sambuddha , Song Minkyung , Adams Levi , Jeong Inhye , Je Goun , Guhathakurta Subhrangshu , Jiang Jennifer , Boparai Nikpreet , Dai Wei , Cardozo-Pelaez Fernando , Tatulian Suren A. , Han Kyu Young , Elliott Jordan , Baum Jean , McLean Pamela J. , Dickson Dennis W. , Kim Yoon-Seong
Published: September 2023

Oxidative stress plays an essential role in the development of Parkinson’s disease (PD). 8-oxo-7,8-dihydroguanine (8-oxodG, oxidized guanine) is the most abundant oxidative stress-mediated DNA lesion. However, its contributing role in underlying PD pathogenesis remains unknown. In this study, we hypothesized that 8-oxodG can generate novel α-synuclein (α-SYN) mutants with altered pathologic aggregation through a phenomenon called transcriptional mutagenesis (TM). We observed a significantly higher accumulation of 8-oxodG in the midbrain genomic DNA from PD patients compared to age-matched controls, both globally and region specifically to α-SYN. In-silico analysis predicted that forty-three amino acid positions can contribute to TM-derived α-SYN mutation. Here, we report a significantly higher load of TM-derived α-SYN mutants from the midbrain of PD patients compared to controls using a sensitive PCR-based technique. We found a novel Serine42Tyrosine (S42Y) α-SYN as the most frequently detected TM mutant, which incidentally had the highest predicted aggregation score amongst all TM variants. Immunohistochemistry of midbrain sections from PD patients using a newly characterized antibody for S42Y identified S42Y-laden Lewy bodies (LB). We further demonstrated that the S42Y TM variant significantly accelerates WT α-SYN aggregation by cell and recombinant protein-based assays. Cryo-electron tomography revealed that S42Y exhibits considerable conformational heterogeneity compared to WT fibrils. Moreover, S42Y exhibited higher neurotoxicity compared to WT α-SYN as shown in mouse primary cortical cultures and AAV-mediated overexpression in the substantia nigra of C57BL/6 J mice. To our knowledge, this is the first report describing the possible contribution of TM-generated mutations of α-SYN to LB formation and PD pathogenesis.
An isoelastic monoblock cup versus a modular metal-back cup: a matched-pair analysis of clinical and radiological results using Einzel-Bild-Röntgen-Analyse software
Authors: Afghanyar Yama , Möller Jens Hendrik , Wunderlich Felix , Dargel Jens , Rehbein Philipp , Gercek Erol , Drees Philipp , Kutzner Karl Philipp
Published: September 2023

Introduction

Bone preservation and long-term survival are the main challenges in cementless total hip arthroplasty (THA). A good bone stock is especially important for adequate anchorage of the cup in revision cases. However, the optimal acetabular cup design for preserving good bone stock is still unclear. We aimed to compare clinical outcome, radiological alterations, migration, and wear at mid-term for two different cup types.

Materials and methods

This retrospective matched-pair study was performed using the data for 98 THA cases treated with a monoblock cup composed of vitamin E-blended highly cross-linked polyethylene (VEPE; monoblock group) or a modular cup composed of a highly cross-linked polyethylene (HXLPE) without an antioxidant (modular group). Clinical results were evaluated using the Harris Hip Score (HHS). The obtained radiographs were analyzed for radiological alterations, migration, and wear using Einzel-Bild-Röntgen-Analyse (EBRA) software.

Results

The mean follow-up duration was 73.2 ± 19.2 months (range: 32–108 months) and 60.5 ± 12.2 months (range: 20–84 months) in the monoblock and modular groups, respectively. HHS improved to 95.7 points in the monoblock group and 97.6 points in the modular group, without significant differences (p = 0.425). EBRA measurements were obtained in all cases. Acetabular bone alterations were not detected on radiological assessments. Mean cup migration was 1.67 ± 0.92 mm (range: 0.46–3.94 mm) and 1.24 ± 0.87 mm (range: 0.22–3.62 mm) in the monoblock and modular groups. The mean wear rate was 0.21 ± 0.18 mm (range: 0.00–0.70 mm) and 0.20 ± 0.13 mm (range: 0.00–0.50 mm) in the monoblock and modular groups. Both migration and wear pattern showed no significant differences (p = 0.741 and 0.243). None of the cases required revision surgery, yielding an implant survival rate of 100% in both groups.

Conclusion

The isoelastic press-fit monoblock VEPE cup and modular metal-back HXLPE cup showed equivalent mid-term wear and cup migration. Long-term studies are required to determine the effects of modularity, isoelasticity, and polyethylene stabilization with vitamin E on cup loosening and survival rates.
Current landscape of hospital information systems in gynecology and obstetrics in Germany: a survey of the commission Digital Medicine of the German Society for Gynecology and Obstetrics
Authors: Pfob André , Griewing Sebastian , Seitz Katharina , Hillen Christoph , Becker Sven , Bayer Christian , Wagner Uwe , Fasching Peter , Wallwiener Markus ,   For the Kommission Digitale Medizin, Deutsche Gesellschaft für Gynäkologie und Gebursthilfe (DGGG), Abele Harald , Alexa Matthias , Cieslik Jan Philipp , Dannehl Dominik , Deutsch Thomas , Fehm Tanja , Graupner Oliver , Hackelöer Max , Hartkopf Andreas , Hein Alexander , Henninsen Maike , Hirsch Martin , Hoffmann Sascha , Hübner Hanna , Hollatz-Galuschki Elsa , Jäger Bernadette , Janse Heike , Kainer Fran , Karsten Maria M. , Kiechle Marion , Lattrich Claus Richard , Schmutzler Andreas , Schulmeyer Elke , Steiner Eric , Volmer Lea Louise , Wallwiener Stephanie , Weichert Jan , Weiß Martin , Wöckel Armin
Published: September 2023

Purpose

Hospital information systems (HIS) play a critical role in modern healthcare by facilitating the management and delivery of patient care services. We aimed to evaluate the current landscape of HIS in the specialty of gynecology and obstetrics in Germany.

Methods

An anonymous questionnaire was distributed via the German Society of Gynecology and Obstetrics newsletter in December 2022. The questionnaire covered the domains baseline demographic information, satisfaction with daily use, satisfaction with implementation, and degree of digitization.

Results

Ninety-one participants completed the survey. Median age was 34 years; 67.4% (60 of 89) were female, and 32.6% (29 of 89) were male. Of the survey participants, 47.7% (42 of 88) were residents, 26.1% (23 of 91) senior physicians, and 9.1% (8 of 88) medical directors. The degree of digitization of clinical documentation is mainly mixed digital and paper-based (64.0%, 57 of 89) while 16.9% (15 of 89) operate mainly paper-based. The current HIS has been in use on average for 9 years. The median number of different software systems used in daily routine is 4. About 33.7% (30 of 89) would likely or very likely recommend their current HIS to a colleague.

Conclusions

The current landscape of HIS in gynecology and obstetrics in Germany is characterized by a high heterogeneity of systems with low interoperability and long service life; thus, many healthcare professionals are not satisfied. There is both a need to enhance and an interest in modernizing the technological infrastructure to meet today’s requirements for patient care.
Targeting metacognitive change mechanisms in acute inpatients with psychotic symptoms: feasibility and acceptability of a modularized group intervention
Authors: Gussmann Eva , Lindner Christoph , Lucae Susanne , Falkai Peter , Padberg Frank , Egli Samy , Kopf-Beck Johannes
Published: September 2023

Emerging evidence suggests the usefulness of psychological interventions targeting metacognitive change mechanisms in patients experiencing psychosis. Although many of these patients are treated in acute psychiatric contexts, only few studies have adapted such interventions for acute inpatient settings. The present study aimed to assess the feasibility, acceptability, and preliminary clinical outcomes of a novel modularized group intervention focusing on different aspects of metacognitive change mechanisms. In particular, the intervention aims to reduce patients’ acute symptoms by enhancing cognitive insight and to relieve distress via cognitive defusion (i.e. coping). A sample of 37 participants with acute psychosis received up to nine sessions of the intervention. Baseline and post-intervention assessments were conducted for general psychopathology, psychotic symptoms, global functioning, and symptom distress. Measures of change mechanisms were assessed before and after the respective treatment module. Participants’ experiences were explored in feedback questionnaires and interviews. Recruitment, retention, and attendance rate met the pre-set feasibility benchmark of 80%. The intervention was well received by participants, who emphasised the group’s clear structure, positive atmosphere, and helpful contents. Response rates were high and linear mixed models revealed significant medium-to-large time effects on all clinical outcomes. As expected, increase in hypothesised change mechanisms cognitive insight and decrease in cognitive fusion was found. However, the uncontrolled design limits interpreting clinical effects. The study provides evidence that an intervention based on a metacognitive model is feasible and acceptable for acute inpatients with psychosis. Positive results on clinical outcomes and change mechanisms warrant further exploration in a randomized controlled trial.
The origin of Na-alkaline lavas revisited: new constraints from experimental melting of amphibole-rich metasomes+lherzolite at uppermost mantle pressure
Authors: Grützner Tobias , Prelević Dejan , Berndt Jasper , Klemme Stephan
Published: September 2023

We present a new experimental dataset for reaction experiments between natural amphibole-clinopyroxene metasomes (hornblendite) and synthetic lherzolite that produced Na-rich alkaline melts. Experiments were conducted at 1, 3 and 4 GPa and 1000–1300 °C. The generated melts range from foidite over basanite to phonotephrite. At 1 GPa between 1000 and 1100 °C amphibole decompression-breakdown products generate a phonotephritic melt. Among the breakdown components rhönite was found to be stable up to 1100 °C and 1 GPa. At 3 and 4 GPa the melt compositions are affected by phlogopite melting and shift to more foiditic compositions. We find that the melting of hornblendites and the reaction of the melt with the lherzolite produce wehrlitic residues with different olivine/clinopyroxene ratios. Wehrlite formation does not always require separate metasomatic processes but can be a direct by-product of alkaline volcanism. We applied a metasome melting model to the magmas of the Kula volcanic province, Turkey, and show that at 1 GPa basanite melts and phonotephrite melts cover the whole range of known Kula lava compositions. The Kula lava compositional trend can be therefore generated by basanite-phonotephrite melt mixing. A comparison of high-pressure (3–4 GPa) melts with natural nephelinite data shows overlap with many major, minor, and trace elements but differences in SiO2, FeO, and TiO2 argue that the natural nephelinite data do not represent primary metasome melts.
Load carriage physiology in normoxia and hypoxia
Authors: Baur Daniel A. , Baur Katherine G. , Buchanan Beverley K. , Ortiz Miles J. , Doody Abaigeal G.
Published: September 2023

Purpose

To determine the effects of load carriage in normoxia and normobaric hypoxia on ventilatory responses, hemodynamics, tissue oxygenation, and metabolism.

Methods

Healthy males (n = 12) completed 3 randomly ordered baseline graded exercise tests in the following conditions: (1) unloaded normoxic (U: FIO2 = 20.93%), (2) loaded (~ 30 kg) normoxic (LN), and (3) loaded hypoxic simulating ~ 3650 m (LH: FIO2 =  ~ 13%). Thereafter, experimental exercise trials were completed in quasi-randomized order (i.e., U completed first) consisting of 3 × 10 min of walking (separated by 5 min seated rest) with stages matched with the U condition (in ascending order) for relative intensity, absolute oxygen consumption ([VO2]; 1.7 L min−1), and walking speed (1.45 ± 0.15 m s−1).

Results

Load carriage increased perceived exertion and reduced VO2max (LN: − 7%; LH: − 32%; p < 0.05). At matched VO2, stroke volume and tidal volume were reduced and maintained with LN and LH vs. U, respectively (p < 0.05). Increases in cardiac output and minute ventilation at matched VO2 (with LH) and speed (with LN and LH), were primarily accomplished via increases in heart rate and breathing frequency (p < 0.05). Cerebral oxygenated hemoglobin (O2HHb) was increased at all intensities with LN, but deoxygenated hemoglobin and total hemoglobin were increased with LH (p < 0.05). Muscle oxygen kinetics and substrate utilization were similar between LN and U, but LH increased CHO dependence and reduced muscle O2HHb at matched speed (p < 0.05).

Conclusion

Load carriage reduces cardiorespiratory efficiency and increases physiological strain, particularly in hypoxic environments. Potential load carriage-induced alterations in cerebral blood flow may increase the risk for altitude illnesses and requires further study.
Development of an ultrasound-assisted pre-treatment strategy for the extraction of d-Limonene toward the production of bioethanol from citrus peel waste (CPW)
Authors: Teke George Mbella , De Vos Liza , Smith Isle , Kleyn Tamryn , Mapholi Zwonaka
Published: September 2023

Citrus is one of the world’s most abundant fruits containing vitamins, pigments, and fragrances, making it vital for several industries. However, these fruits contain about 45–50% residues (peels), which often end up as waste and can be harmful to the environment if not properly treated. Bioethanol production from citrus peel waste offers a potential solution to this problem. Hence, this study explores the potential of using ultrasound-assisted pre-treatment method as a novel strategy to extract d-Limonene (essential oil in the residue), and further demonstrates bioethanol production. This was done by investigating ultrasonication’s optimal effect on pre-treatment of the citrus residue, followed by bioethanol production. The results show that, optimum values for d-Limonene extraction were obtained at a temperature of 14.6 °C and an ultrasound intensity of 25.81 W/cm2 with a validation yield of 134 ± 4.24 mg/100 g dry CPW. With optimal ultrasonic parameters, the study went further to demonstrate the effect of the essential oil on bioethanol production which is hindered by the oils present. Key findings show better bioethanol yield once the essential oil was extracted (treated) from the citrus waste as opposed to it not extracted (untreated), with a 66 and a 29% increase when comparing simultaneous saccharification and fermentation (SSF) and sequential hydrolysis and fermentation (SHF) respectively. Based on this result, ultrasound-assisted extraction as a pretreatment method was found suitable for bioethanol production from citrus residue and could be utilized as a biorefinery pre-treatment approach to scale bioethanol production.
Pelvic floor muscle training delivered via telehealth to treat urinary and/or faecal incontinence after gynaecological cancer surgery: a single cohort feasibility study
Authors: Brennen Robyn , Soh Sze-Ee , Denehy Linda , Lin Kuan Yin , Jobling Thomas , McNally Orla M. , Hyde Simon , Kruger Jenny , Frawley Helena
Published: September 2023

Purpose

To assess the feasibility and clinical outcomes of telehealth-delivered pelvic floor muscle training (PFMT) for urinary incontinence (UI) and/or faecal incontinence (FI) after gynaecological cancer surgery.

Methods

In this pre-post cohort clinical trial, patients with incontinence after gynaecological cancer surgery underwent a 12-week physiotherapist-supervised telehealth-delivered PFMT program. The intervention involved seven videoconference sessions with real-time feedback from an intra-vaginal biofeedback device and a daily home PFMT program. Feasibility outcomes included recruitment, retention, engagement and adherence rates. Clinical outcomes were assessed at baseline, immediately post-intervention and a 3-month post-intervention using International Consultation on Incontinence questionnaires for UI (ICIQ-UI-SF) and Bowel function (ICIQ-B) and the intra-vaginal biofeedback device. Means and 95%CIs for all time points were analysed using bootstrapping methods.

Results

Of the 63 eligible patients, 39 (62%) consented to the study. Three participants did not complete baseline assessment and were not enrolled in the trial. Of the 36 participants who were enrolled, 32 (89%) received the intervention. Retention was 89% (n=32/36). The majority of participants (n=30, 94%) demonstrated high engagement, attending at least six videoconference sessions. Adherence to the daily PFMT program was moderate, with 24 participants (75%) completing five-to-seven PFMT sessions per week during the intervention. All clinical outcomes improved immediately post-intervention; however, the magnitude of these improvements was small.

Conclusion

Telehealth-delivered PFMT may be feasible to treat incontinence after gynaecological cancer surgery.

Trial registration

ClinicalTrials.gov Identifier: ACTRN12621000880842)
Glucose-6-phosphate dehydrogenase deficiency accelerates arterial aging in diabetes
Authors: Scuteri Angelo , Morrell Christopher H. , AlGhatrif Majd , Orru Marco , Fiorillo Edoardo , Marongiu Michele , Schlessinger David , Cucca Francesco , Lakatta Edward G.
Published: September 2023

Aims

High glucose levels and Glucose-6-Phosphate Dehydrogenase deficiency (G6PDd) have both tissue inflammatory effects. Here we determined whether G6PDd accelerates arterial aging (information linked stiffening) in diabetes.

Methods

Plasma glucose, interleukin 6 (IL6), and arterial stiffness (indexed as carotid-femoral Pulse Wave Velocity, PWV) and red blood cell G6PD activity were assessed in a large (4448) Sardinian population.

Results

Although high plasma glucose in diabetics, did not differ by G6DP status (178.2 ± 55.1 vs 169.0 ± 50.1 mg/dl) in G6DPd versus non-G6PDd subjects, respectively, IL6, and PWV (adjusted for age and glucose) were significantly increased in G6PDd vs non-G6PDd subjects (PWV, 8.0 ± 0.4 vs 7.2 ± 0.2 m/sec) and (IL6, 6.9 ± 5.0 vs 4.2 ± 3.0 pg/ml). In non-diabetics, neither fasting plasma glucose, nor IL6, nor PWV were impacted by G6PDd.

Conclusion

G6PDd in diabetics is associated with increased inflammatory markers and accelerated arterial aging.
Future photovoltaic solar power resources in Zambia: a CORDEX-CORE multi-model synthesis
Authors: Libanda Brigadier , Paeth Heiko
Published: September 2023

The exploration of renewable energy such as wind and solar radiation has the potential of reducing reliance on fossil fuels, thus cutting emissions of carbon dioxide, particulate matter, and several other greenhouse gasses. However, recent findings indicate that wind speed across Zambia is very slow, it is increasing but remains unlikely to support large commercial wind farms. In this study, we explore the future impacts of climate change on solar photovoltaic resources. To do this, we examine the new high-resolution (25 km) Coordinated Regional Climate Downscaling Experiment—CORDEX-CORE simulations for the African domain, using two different emission scenarios until 2100. At an annual scale, results indicate a weak but steady decrease in PVRes of around 0.02 W/m2 per annum under RCP2.6 and about 0.005 W/m2 per annum under RCP8.5. Results further show that at an average of ~ 237 ± 3.3 W/m2 and 212 ± 2.5 W/m2, respectively, RCP2.6 comes along with 12 ± 3% more PVRes than RCP8.5. Thus RCP2.6, a greener and climate-friendly pathway, points towards a higher renewable energy potential across Zambia compared to the business-as-usual pathway.
Survival and success of zirconia compared with titanium implants: a systematic review and meta-analysis
Authors: Padhye Ninad Milind , Calciolari Elena , Zuercher Anina Nives , Tagliaferri Sara , Donos Nikos
Published: September 2023

Objective

This systematic review assessed the available evidence on the survival and success rate of zirconia and titanium implants. As secondary outcomes, aesthetic, radiographic and clinical parameters, as well as biological and mechanical complications, were considered.

Materials and methods

A systematic search was performed up to March 2022 to identify CCTs/RCTs comparing zirconia and titanium implants with a minimum of 12 months of follow-up. Meta-analysis was performed when ≥ 2 articles with similar characteristics were retrieved.

Results

Four published articles with two RCTs (2 different patient populations) with 100 zirconia and 99 titanium implants that were followed up over 12–80 months were selected out of the 6040 articles. A non-statistically significant difference between zirconia and titanium implant survival at 12 months was suggested (P = 0.0938). The success rates were 57.5–93.3% and 57.1–100% for zirconia and titanium implants, respectively. The pink aesthetic score (PES) was higher for zirconia (10.33 ± 2.06 to 11.38 ± 0.92) compared to titanium implants (8.14 ± 3.58 to 11.56 ± 1.0).

Conclusion

Based on the 2 RCTs retrieved in the literature, similar survival rates were reported for zirconia and titanium implants in the short term (12 months of follow-up). Future RCTs are warranted to evaluate the long-term outcomes of zirconia implants.

Clinical relevance

Zirconia implants may be the procedure of choice, particularly in the aesthetic zone, since they show a similar survival and success rate as titanium implants on a short-term follow-up.

Trial registration

Systematic review registration number—CRD42021288704 (PROSPERO).
Longitudinal relationships of psychotic-like experiences with suicidal ideation and self-harm in adolescents
Authors: Zhou Rui , Foo Jerome Clifford , Nishida Asuka , Ogawa Sayoko , Togo Fumiharu , Sasaki Tsukasa
Published: September 2023

Research in adolescents suggests associations between psychotic-like experiences (PLEs) and self-injurious thoughts and behaviors (SITBs), but insights into their temporal relationship, which may inform prediction, have been limited. Psychological distress (PD; symptoms of depression and anxiety) has been related to both PLEs and SITBs, and may modulate this relationship. Given that PLEs have been linked to the development of several mental disorders, and the relationships between SITBs and suicide, it is important to better understand their relationship. The present study sought to investigate these factors using a longitudinal school-based design. Adolescents (n = 1685, ages 12–18) completed annual self-report assessments (6 time points) on PLEs, SITBs (suicidal ideation (SI) and self-harm (SH)), as well as PD. The longitudinal associations between PLEs and SITBs were analyzed, employing two cross-lagged panel models (CLPMs), with and without adjustment for PD. Unadjusted CLPMs revealed significant bidirectional temporal associations between PLEs and SITBs (both SI and SH), suggesting that PLEs both predicted and were predicted by SITBs. When adjusting for PD, the effect of SI on PLEs remained significant, but not PLEs on SI; bidirectional associations between PLEs and SH also remained significant. A bidirectional longitudinal relationship where both PLEs and SITBs can precede (and perhaps predict) each other was suggested in adolescents. PD may play a particular role in situations where PLEs are followed by SI. Heightened awareness about relationships between these phenotypes may be an important step toward facilitating timely interventions for both mental disorders and suicide.
Does orthognathic surgery affect mandibular condyle position? A retrospective study
Authors: Dvoranova Bronislava , Vavro Michal , Czako Ladislav , Hirjak Dusan
Published: September 2023

Purpose

The aim of this study is to analyze mandibular condyle position changes after bilateral sagittal split osteotomy (BSSO) and bimaxillary orthognathic surgery in patients operated at a single department by two surgeons in 2013–2022. Compared were groups of mandibular advancement vs setback and bimaxillary vs BSSO.

Methods

Ninety-nine subjects were included. Inclusion criteria were patients who underwent one of the BSSO or bimaxillary surgery and had CT scans performed before and after surgery. Preoperative CT scans were performed 1 day before surgery and postoperative CT scans 6–12 months afterwards. Changes in mandibular condyle position were measured in axial and sagittal planes.

Results

CT condylar position measurements indicated significant postoperative changes in AB angle bilaterally (p =  < 0.001). In mandibular advancement and setback comparison, values were significantly lower in ABL angle values in the setback group (p = 0.011326) and significantly higher in FDR in the advancement group (p = 0.005795). There were no statistically significant changes found in BSSO and bimaxillary group comparison.

Conclusion

Within the limitations of this study, it can be concluded that orthognathic surgery does have a moderate effect on position of the condyles, especially condylar rotation in transversal axis.
Leaf morphometric analysis and potential distribution modelling contribute to taxonomic differentiation in the Quercus microphylla complex
Authors: De Luna-Bonilla Oscar Ángel , Valencia-Á Susana , Ibarra-Manríquez Guillermo , Morales-Saldaña Saddan , Tovar-Sánchez Efraín , González-Rodríguez Antonio
Published: September 2023

Mexico is a major center of evolutionary radiation for the genus Quercus, with oak species occurring across different habitat types and showing a wide variation in morphology and growth form. Despite representing about 20% of Mexican species, scrub oaks have received little attention and even basic aspects of their taxonomy and geographic distribution remain unresolved. In this study, we analyzed the morphological and climatic niche differentiation of scrub oak populations forming a complex constituted by six named species, Quercus cordifolia, Quercus frutex, Quercus intricata, Quercus microphylla, Quercus repanda, Quercus striatula and a distinct morphotype of Q. striatula identified during field and herbarium work (hereafter named Q. striatula II). Samples were obtained from 35 sites covering the geographic distribution of the complex in northern and central Mexico. Morphological differentiation was analyzed through geometric morphometrics of leaf shape and quantification of trichome traits. Our results indicated the presence of two main morphological groups with geographic concordance. The first was formed by Q. frutex, Q. microphylla, Q. repanda and Q. striatula, distributed in the Trans-Mexican Volcanic Belt, the Sierra Madre Occidental and a little portion of the south of the Mexican Altiplano (MA). The second group consists of Q. cordifola, Q. intricata and Q. striatula II, found in the Sierra Madre Oriental and the MA. Therefore, our evidence supports the distinctness of the Q. striatula II morphotype, indicating the need for a taxonomic revision. Within the two groups, morphological differentiation among taxa varied from very clear to low or inexistent (i.e. Q. microphylla-Q. striatula and Q. cordifolia-Q. striatula II) but niche comparisons revealed significant niche differentiation in all pairwise comparisons, highlighting the relevance of integrative approaches for the taxonomic resolution of complicated groups such as the one studied here.
Evaluation of levator ani muscle elasticity after vaginal delivery and cesarean section using shear wave elastography
Authors: Okada Yoshiyuki , Nakagawa Chie , Shigeta Miwa , Nomura Yukiko , Inoue Eisuke , Ichizuka Kiyotake , Yoshimura Yasukuni
Published: September 2023

Purpose

The risk of pelvic floor muscle injury is commonly considered to be higher in vaginal than in cesarean delivery. This study aimed to compare levator ani muscle (LAM) elasticity after vaginal and cesarean delivery using shear wave elastography (SWE).

Methods

Postpartum women who underwent a single SWE evaluation 1 month after their first delivery were divided into vaginal and cesarean delivery groups. The elastic moduli of both sides of the LAM were measured in a horizontal section and compared between the groups. In addition, a subgroup analysis was performed to compare LAM elasticity according to the delivery method within the vaginal delivery group—normal vaginal delivery, episiotomy, and operative vaginal delivery.

Results

Sixty-two women were included (vaginal delivery, n = 47; elective cesarean section, n = 15). Multiple regression analysis revealed that the LAM elastic modulus was significantly lower in the vaginal delivery group than in the cesarean delivery group (right LAM: 44.2 vs. 72.7 kPa, p = 0.0036; left LAM 40.4 vs. 82.7 kPa, p < 0.0001). In the subgroup analysis, the right LAM elastic modulus was significantly lower in the operative vaginal delivery subgroup than in the normal vaginal delivery subgroup (p = 0.0131). However, there was no significant difference in the left LAM elastic modulus between the three subgroups.

Conclusion

LAM elasticity was significantly lower after vaginal delivery than after cesarean delivery. Furthermore, the elasticity of the right LAM was lower after operative vaginal delivery than after normal vaginal delivery. SWE has the potential to provide an objective quantitative assessment of postpartum pelvic floor muscle recovery.
Producer and consumer perspectives on supporting and diversifying local food systems in central Iowa
Authors: Dorneich Michael C. , Krejci Caroline C. , Schwab Nicholas , Stone Tiffanie F. , Huckins Erin , Thompson Janette R. , Passe Ulrike
Published: September 2023

The majority of food in the US is distributed through global/national supply chains that exclude locally-produced goods. This situation offers opportunities to increase local food production and consumption and is influenced by constraints that limit the scale of these activities. We conducted a study to assess perspectives of producers and consumers engaged in food systems of a major Midwestern city. We examined producers’ willingness to include/increase cultivation of local foods and consumers’ interest in purchasing/increasing local foods. We used focus groups of producers (two groups of conventional farmers, four local food producers) and consumers (three conventional market participants, two locavores) to pose questions about production/consumption of local foods. We transcribed discussions verbatim and examined text to identify themes, using separate affinity diagrams for producers and consumers. We found producers and consumers are influenced by the status quo and real and perceived barriers to local foods. We also learned participants believed increasing production and consumption of local foods would benefit their community and creating better infrastructure could enhance efforts to scale up local food systems. Focus group participants also indicated support from external champions/programs could support expansion of local foods. We learned that diversifying local food production was viewed as a way to support local community, increase access to healthy foods and reduce environmental impacts of conventional production. Our research indicates that encouraging producers and consumers in local food systems will be more successful when support for the local community is emphasized.
An oak is an oak, or not? Understanding and dealing with confusion and disagreement in biological classification
Authors: Cuypers Vincent , Reydon Thomas A. C.
Published: September 2023

Human interaction with the living world, in science and beyond, always involves classification. While it has been a long-standing scientific goal to produce a single all-purpose taxonomy of life to cater for this need, classificatory practice is often subject to confusion and disagreement, and many philosophers have advocated forms of classificatory pluralism. This entails that multiple classifications should be allowed to coexist, and that whichever classification is best, is context-dependent. In this paper, we discuss some practical consequences of classificatory pluralism, in particular with regard to how one is supposed to find the best classification for a given context. We do so by means of a case study concerning oaks, in particular the pedunculate oak (Quercus robur L.) and the sessile oak (Quercus petraea (Matt.) Liebl.), two important putative species that present several classificatory challenges; and by applying one recent philosophical framework conceptualizing classification, the so-called Grounded Functionality Account (GFA) of (natural) kinds. We show how the GFA elucidates several issues related to oak classification and gives directions to optimize classificatory practices, and discuss some implications for scientific taxonomy.
Factors of green entrepreneurship in selected emerging markets in the European Union
Authors: Misztal Anna , Kowalska Magdalena
Published: September 2023

The paper researches factors influencing green entrepreneurship in the five European Union's emerging markets from 2008 to 2020. We choose five countries from the UE not in the eurozone (International Monetary Fund's approach to assessing economies), including Bulgaria, Croatia, Hungary, Poland, and Romania. The theoretical part discusses selected problems related to green entrepreneurship (we focus on analysing green sectors of the economy) and its determinants. The practical part shows the research results. We use Pearson's R, the ordinary least squares, and the vector autoregression model (VAR) to verify our research hypothesis (p-value < 0.05). We focus on factors which are crucial for entrepreneurship OECD (2011). Measuring green entrepreneurship. Entrepreneurship at a Glance, including macroeconomic conditions (MacroCon), creation and diffusion of knowledge (CaDKnow), entrepreneurial skills and capabilities (CapEnt), regulations (RegFram), and the expenditure on environmental protection (EnvProt). According to the OLS regression, there is a substantial variation in the impact of individual factors on green entrepreneurship in emerging markets from 2010 to 2020. Moreover, green is conditioned by a sequence of events, with the past determining the present values.
Calculation of Dark Matter as a Feature of Space–Time
Authors: Handel Peter H. , Splett Klara E.
Published: September 2023

We derive the first analytical formula for the density of "Dark Matter" (DM) at all length scales, thus also for the rotation curves of stars in galaxies, for the baryonic Tully–Fisher relation and for planetary systems, from Einstein's equations (EE) and classical approximations, in agreement with observations. DM is defined in Part I as the energy of the coherent gravitational field of the universe, represented by the additional equivalent ordinary matter (OM), needed at all length scales, to explain classically, with inclusion of the OM, the observed coherent gravitational field. Our derivation uses both EE and the Newtonian approximation of EE in Part I, to describe semi-classically in Part II the advection of DM, created at the level of the universe, into galaxies and clusters thereof. This advection happens proportional with their own classically generated gravitational field g, due to self-interaction of the gravitational field. It is based on the universal formula ρD = λgg′2 for the density ρD of DM advected into medium and lower scale structures of the observable universe, where λ is a universal constant fixed by the Tully–Fisher relations. Here g′ is the gravitational field of the universe; g′ is in main part its own source, as implied in Part I from EE. We start from a simple electromagnetic analogy that helps to make the paper generally accessible. This paper allows for the first time the exact calculation of DM in galactic halos and at all levels in the universe, based on EE and Newtonian approximations, in agreement with observations.
Morphology tuned plasmonic TiN nanostructures formed by angle-dependent sputtering process for SERS measurements
Authors: Barman Tapan , Nozka Libor , Prochazka Vit , Michałowska Aleksandra , Turczyniak-Surdacka Sylwia , Ctvrtlik Radim , Krajczewski Jan
Published: September 2023

Nowadays, most SERS platforms are based on plasmonic metals like silver and gold. The current scientific achievement is to construct SERS non-metallic platform with SERS activity not worse than in case of standard metallic platforms. The prominent combination of opto-electronic properties of TiN (titanium nitride) makes it a promising alternative plasmonic material for noble metals. So far, the TiN nanostructures are widely tested in catalysis, especially in photocatalysis. Despite the TiN potential in SERS sensing applications, there are still several fundamental issues preventing its wide practical application. In this work, the surface morphology of TiN layers was tuned from planar 2D to a high aspect ratio 1D nanorod-like structures by industrially scalable normal and glancing angle deposition (GLAD) pulsed DC reactive sputtering. The morphology of formed samples was studied by SEM microscopy, while the crystallographic properties were examined by Raman and XRD method. The opto-electrical parameters measured by spectroscopic ellipsometry, and DRS spectroscopy revealed substantial changes in optical properties of the TiN films with the variation of glancing angle. This in turn opened the way for tailoring the surface plasmon resonance and hence for SERS activity.
Association of weight and shape concern with weight change and weight-related behaviors in behavioral weight loss treatment
Authors: Goldstein Stephanie P. , Olson KayLoni L. , Thomas J. Graham
Published: September 2023

Weight and shape concern (WSC) is a facet of negative body image that is common among individuals with overweight/obesity seeking behavioral weight loss treatment (BWL), but remains understudied. This secondary analysis evaluates associations between WSC, weight change, and weight-related behaviors among individuals in a 24-week BWL. Adults (n = 32) with body mass index 25–50 kg/m2 completed a baseline WSC questionnaire, measured weight at 12 and 24 weeks, measured physical activity via accelerometer, and completed 24-hour dietary recalls. Adherence to self-monitoring dietary intake and weight were assessed. A series of linear mixed models were used to evaluate associations between baseline WSC and weight change, as well as weight-related behaviors. Results revealed no significant effect of WSC on weight change. There were significant WSC x time interactions, such that those rating WSC “very important” decreased self-weighing and the “low importance” group decreased their caloric intake during treatment. The “pretty important” group had greater minutes of activity than the “low importance” group. Findings indicated that WSC may impact weight-related behaviors that contribute to BWL success. This trial was pre-registered on ClinicalTrials.gov (NCT03739151).
The Certified QPR Pathfinder Training Program: A Description of a Novel Public Health Gatekeeper Training Program to Mitigate Suicidal Ideation and Suicide Deaths
Authors: Quinnett Paul G.
Published: September 2023

Suicide represents a significant public health concern. One approach to reducing suicide rates is to train gatekeepers—community members who, through their day-to-day practices, interact with a substantial proportion of the population—to detect individuals at elevated suicide risk and refer them to appropriate mental health care services. One of the most well-known community gatekeeper training programs is Question, Persuade, and Refer (QPR), which has been delivered to millions worldwide. Gatekeeper training, including QPR, shows considerable promise in reducing suicide risk. However, one limitation of existing gatekeeper training programs is that they rely on referrals to mental health services, which are often non-existent, understaffed, and/or undertrained regarding suicide risk. As such, novel approaches are needed to equip community gatekeepers with primary mental health first aid and suicide-focused counseling. This article describes, for the first time, the fundamental concepts of a newly developed and more expansive version of QPR, the QPR Pathfinder Training. The QPR Pathfinder Training is web-based training program designed to create a cadre of “super gatekeepers” to address suicide at scale. The QPR Pathfinder Training will equip communities to address the critical shortage of mental health care services around the globe and, in turn, reduce mental health morbidities and decrease the suicide rates.
Co-design and Development of Implementation Strategies: Enhancing the PAX Good Behaviour Game in Australian Schools
Authors: Baffsky Rachel , Ivers Rebecca , Cullen Patricia , McGillivray Lauren , Werner-Seidler Aliza , Calear Alison L. , Batterham Philip J. , Toumbourou John W. , Stokes Rhoni , Kotselas Pauline , Prendergast Traci , Torok Michelle
Published: September 2023

Effective implementation strategies are important for take-up of programs in schools. However, to date, few implementation strategies have been co-designed with teachers and support staff (including principals) in Australia. The aim of this study was to iteratively co-design multiple implementation strategies to enhance the delivery of mental health prevention program, PAX Good Behaviour Game, in New South Wales primary schools. The secondary aim was to evaluate the acceptability of the implementation strategies from the perspective of school staff. Twenty-nine educational staff (including principals) informed the co-design of the implementation strategies across three phases. Phase 1 involved a rapid review of the literature and stakeholder meetings to agree upon potential evidence-based strategies. Phase 2 involved focus group discussions with educational staff to co-design implementation strategies. Phase 3 involved semi-structured interviews with school staff to assess strategy acceptability after implementation at 6-months post-baseline. Data were analysed using deductive, framework analysis. The final co-designed intervention included nine implementation strategies accessible through a toolkit delivered to the school’s leadership team. These strategies were deemed acceptable in school settings that experienced periods of both face-to-face and remote learning due to the changing COVID-19 situation in 2021. This paper contributes to the implementation literature by transparently reporting how educational staff-informed implementation strategies were iteratively co-designed. This will provide a roadmap for other researchers to co-design implementation strategies to further support the delivery of evidence-based prevention programs in schools.
Investigation of different ways of activation of fly ash–cement mixtures: part 2—mechanical activation
Authors: Wilińska Iwona , Pacewska Barbara , Ostrowski Andrzej
Published: September 2023

Introducing supplementary cementitious materials (SCM), e.g. fly ash, into cement composite results in ecological benefits. However, in the case of high amount of SCM used as a replacement of a part of cement, there are problems related to the development of the desired properties of the final composite. Such mixtures often require activation. In the first part of this series of publications, the results of chemical activation (using Na2SO4 and Ca(OH)2) of a mixture with a very high content of fly ash were discussed. The aim of this work was to investigate the influence of mechanical activation on hydration and microstructure of the binder composed of 80% of fly ash and 20% of cement. Mechanical activation was performed using a planetary ball mill. The following instrumental methods were used to investigate the activated fly ash-cement pastes: calorimetry, TG/DTG, FTIR spectroscopy, X-ray diffraction and scanning electron microscopy (SEM) coupled with EDS. It was shown that concomitant grinding of cement and fly ash is more effective compared to separate grinding. Mechanism of hydration/activation of such mixtures was discussed in detail.
Effectiveness of natural biomaterials in the protection and healing of experimentally induced gastric mucosa Ulcer in rats
Authors: Abdel-Gawad Doaa R.I. , Ibrahim Marwa A. , Moawad Usama K. , Kamel Shaimaa , El-Banna Hossny A. , El-Banna Ahmed H. , Hassan Walid Hamdy , El-Ela Fatma I. Abo
Published: September 2023

Background

A gastric ulcer is a painful lesion of the gastric mucosa that can be debilitating or even fatal. The effectiveness of several plant extracts in the therapy of this illness has been demonstrated in traditional pharmacopoeias. Aim: this study was aimed to see if propolis, ginseng in normal or nano form, and amygdalin might help in preventing the ulcerative effects of absolute ethanol.

Methods

Gastroprotective properties of pretreatments before ethanol gavage in rats were compared to omeprazole. The ulcer and stomach parameters (ulcerated regions) were measured (mm2), ulcer inhibition percentage, the stomachs were assessed macroscopically with gastric biopsy histological examinations.

Results

Amygdalin, normal and nano ginseng, nano propolis followed by propolis all showed great efficacy in protecting the cyto-architecture and function of the gastric mucosa. The number of ulcerated sites was greatly reduced, and the percentage of stomach protection was increased. Histopathological examination had confirmed great protective effects of the nanoformulations followed by amygdalin. The protection and healing rate was completed to about 100% in all tested materials while ulcer areas were still partially unhealed in normal propolis and omeprazole. Quantitative assay of the m-RNA levels Enothelin 1(ET-1), leukotriene4 (LT-4), and caspase 3(Cas-3) genes and Histamine were done and revealed significant up-regulations in ethanol group and the maximum protective effect was reported with ginseng nano, moreover the histamine content was significantly decreased with nano- formulated extracts.

Conclusion

Amygdalin and the nanoformulated ginseng and propolis had exhibited a marked protective effect against the ulcerative toxic effects of ethanol.
Repurposing fexofenadine as a promising candidate for diabetic kidney disease: randomized clinical trial
Authors: El-fatatry Basma Mahrous , El-Haggar Sahar Mohamed , Ibrahim Osama Mohamed , Shalaby Khaled Hamed
Published: September 2023

Purpose

Diabetic kidney disease (DKD) is a devastating complication of diabetes mellitus. Inflammation and histamine are potentially involved in the disease progression. This study aimed to evaluate the role of fexofenadine in patients with DKD.

Methods

From January 2020 to February 2022, out of 123 patients screened for eligibility, 61 patients completed the study. Patients were randomized into two groups, the fexofenadine group (n = 30): received ramipril plus fexofenadine, and the control group (n = 31): received ramipril only for six months. Changes in urinary albumin to creatinine ratio (UACR) and estimated glomerular filtration rate (eGFR) were considered primary outcomes. Measurements of urinary cyclophilin A, monocyte chemoattractant protein-1 (MCP-1), 8-hydroxy-2′ deoxyguanosine (8-OHdG), and podocalyxin (PCX) were considered secondary outcomes. The study was prospectively registered on clinicaltrial.gov on January 13, 2020, with identification code NCT04224428.

Results

At the end of the study, fexofenadine reduced UACR by 16% (95% CI, − 23.4% to − 9.3%) versus a noticeable rise of 11% (95% CI, 4.1% to 17.8%) in UACR in the control group, (p < 0.001). No significant difference in eGFR was revealed between the two groups. However, the control group showed a significant decrease of − 3.5% (95% CI, − 6.6% to − 0.3%) in eGFR, compared to its baseline value. This reduction was not reported in the fexofenadine group. Fexofenadine use was associated with a significant decline in MCP-1, 8-OHdG, and PCX compared to baseline values.

Conclusion

Fexofenadine is a possible promising adjuvant therapy in patients with DKD. Further large-scale trials are needed to confirm our preliminary results.