bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping and Assessing Cultural Ecosystem Services to Inform Maritime Spatial Planning: A Systematic Review
Authors: Banela Marina , Kyvelou Stella Sofia , Kitsiou Dimitra
Published: February 2024

Socio-cultural dimensions and values in maritime spatial planning (MSP) are gaining momentum worldwide and especially in the European Union (EU), since it is acknowledged that they are the “missing layer” of maritime spatial plans (MSPlans). These values are partly explored through cultural ecosystem services (CES). Coastal and marine CES refer to the benefits that humans derive from the coastal and marine environments in recreational, artistic, spiritual, and educational terms. They reflect the ways in which coastal and marine ecosystems contribute to human well-being, cultural identity, and interactions with nature. The research question is how to map and assess coastal/marine ecosystem services and to integrate them in the MSPlans. Thus, this systematic review sheds light on methodological and technical issues with CES mapping and assessment and provides guidance on how to incorporate CES in MSP for future research. Publications dealing with the spatial aspects of CES were examined with an extra focus on participatory mapping approaches. Initially, 199 articles were identified, and 50 articles were ultimately eligible for the analysis. Papers were concentrated in the European Atlantic, whilst their scope was either local or regional. A large number of articles consider the contribution of CES to efficient marine management rather than to achieving an integrated and coordinated MSP. Social and mixed mapping approaches were met with the most often, especially participatory mapping approaches. Also, non-monetary valuation was prevailing, and a multitude of techniques, tools, and statistical software were used. In most of the cases, the distribution of values in relation to environmental, socio-economic, and demographic factors was examined. This article concludes that an effective integration of CES into MSP is crucial for creating prospects of a more sustainable and inclusive approach to coastal/marine planning and for the well-being of ecosystems and communities both currently and for future generations. This knowledge should become “property” of the maritime spatial planners.
The Minimisation of Cardiovascular Disease Screening for Kidney Transplant Candidates
Authors: Corr Michael , Orr Amber , Courtney Aisling E.
Published: February 2024

Background: There is increasing evidence that cardiac screening prior to kidney transplantation does not improve its outcomes. However, risk aversion to perioperative events means that, in practice, testing remains common, limiting the availability of ‘real-world’ data to support any change. Our objective was to assess perioperative and 1-year post-transplant cardiovascular events in a kidney transplant candidate cohort who received minimal cardiovascular screening. Methods: The retrospective cohort study included all adult kidney-only transplant recipients in a single UK region between January 2015 and December 2021. Kidney transplant recipients asymptomatic of cardiac disease, even those with established risk factors, did not receive cardiac stress testing. The perioperative and 1-year post-transplant cardiovascular event incidences were examined. Logistic regression was used to identify variables of statistical significance that predicted cardiovascular or cerebrovascular events. Results: A total of 895 recipients fulfilled the inclusion criteria. Prior to transplantation, 209 (23%) recipients had an established cardiac diagnosis, and 193 (22%) individuals had a diagnosis of diabetes. A total of 18 (2%) patients had a perioperative event, and there was a 5.7% incidence of cardiovascular events 1 year post-transplantation. The cardiovascular mortality rate was 0.0% perioperatively, 0.2% at 3 months post-transplant, and 0.2% at 1 year post-transplant. Conclusions: This study demonstrates comparable rates of cardiovascular events despite reduced screening in asymptomatic recipients. It included higher risk individuals who may, on the basis of screening results, have been excluded from transplantation in other programmes. It provides further evidence that extensive cardiac screening prior to kidney transplantation is unlikely to be offset by reduced rates of cardiovascular events.
Sodium-Glucose Co-Transporter 2 Inhibitors: Mechanism of Action and Efficacy in Non-Diabetic Kidney Disease from Bench to Bed-Side
Authors: Abdelrahman Aly M. , Awad Alaa S. , Abdel-Rahman Emaad M.
Published: February 2024

Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are currently available for the management of type 2 diabetes mellitus. SGLT2i acts by inhibiting renal SGLT2, thereby increasing glucosuria and lowering serum glucose. Recent trials are emerging supporting a role for SGLT2i irrespective of the diabetic status pointing towards that SGLT2i have other mechanisms of actions beyond blood sugar control. In this review, we will shed light on the role of this group of medications that act as SGLT2i in non-diabetics focusing on pre-clinical and clinical data highlighting the mechanism of renoprotection and effects of SGLT2i in the non-diabetic kidneys.
Ultrasonic-Vibration-Superimposed Face Turning of Aluminium Matrix Composite Components for Enhancing Friction-Surface Preconditioning
Authors: Eiselt Patrick , Hirsch Sarah Johanna , Ozdemir Ismail , Nestler Andreas , Grund Thomas , Schubert Andreas , Lampke Thomas
Published: February 2024

Aluminium matrix composites (AMCs) represent an important group of high-performance materials. Due to their specific strength and a high thermal conductivity, these composites have been considered for the large-scale production of brake discs. However, preconditioning the friction surfaces is necessary to avoid severe wear of both the brake discs and the brake linings. This can be achieved through controlled friction against commercially available brake-lining materials and the formation of transfer or reactive layers (tribosurfaces). Homogeneous tribosurfaces allow for nearly wear-free brake systems under moderate brake conditions. In this work, preconditioning was carried out with a pin-on-disc tester, aiming for the fast creation of homogeneously formed and stable tribosurfaces. The influence of surface microedges perpendicular to the direction of friction on the machined AMC surfaces on the build-up speed and homogeneity of the tribosurfaces was investigated. The microedges were generated using ultrasonic-vibration-superimposed face turning. Thereby, the vibration direction corresponded to the direction of the passive force. For research purposes, the distance of the microedges was changed by varying the cutting speed and feed. The experiments were carried out using AMC disc specimens with a reinforcement content of a 35% volume proportion of silicon carbide particles. Machining was realised with CVD-diamond-tipped indexable inserts. The evaluation of the generated surfaces before and after preconditioning was achieved using 3D laser scanning microscopy and scanning electron microscopy. It was demonstrated that ultrasonic-vibration-superimposed face turning effectively generated microedges on the AMC surfaces. The results show that larger distances between the microedges enhanced the formation of stable tribosurfaces. Thus, the tribosystem’s steady state was reached quickly. Therefore, the benefits of AMC-friction-surface microstructuring on the generation of tribosurfaces under laboratory conditions were proven. These findings contribute to the development of high-performance AMC brake systems.
Numerical Simulations of Seaplane Ditching on Calm Water and Uniform Water Current Coupled with Wind
Authors: Zha Ruosi , Wang Kai , Sun Jianglong , Tu Haiwen , Hu Qi
Published: February 2024

In this paper, the ditching performance of a seaplane model on calm water and a uniform water current coupled with wind was numerically investigated. The overset grid technique was applied to treat the large amplitude of the body motions of the seaplane without leading to mesh distortion. The effects of the initial velocity and the initial pitch angle on the slamming loads and motion responses were investigated for the seaplane’s ditching on calm water. A good agreement with the experimental data on the velocity and angle was obtained. Besides ditching on calm water without the water current and wind, three more-complicated conditions were adopted, including the seaplane’s ditching on calm water with wind, a water current without wind, and a water current coupled with wind. The accelerations and impact pressures of the seaplane can be influenced by the wind or current. Water splashing and overwashing could be observed during the water entry process, with water overtopping the seaplane head or nose and flowing over the body surface. It can be concluded that the relative motion between the water and the seaplane model should be carefully controlled to avoid possible damages caused by the occurrence of overwashing.
Reaction Capsule Design for Interaction of Heavy Liquid Metal Coolant, Fuel Cladding, and Simulated JOG Phase at Accident Conditions
Authors: Tarı Doğaç , Retegan Vollmer Teodora , Geers Christine
Published: February 2024

High temperature corrosion of fuel cladding material (15-15Ti) in high burn-up situations has been an important topic for molten metal-cooled Gen-IV reactors. The present study aims to investigate the simultaneous impact of liquid lead (coolant side) and cesium molybdate (fuel side) on the cladding tube material. A capsule was designed and built for experiments between 600 °C and 1000 °C. In order to simulate a cladding breach scenario, a notch design on the cladding tube was investigated pre- and postexposure. Material thinning by corrosion and leaching at temperatures ≥ 900 °C caused breaches at the notches after 168 h exposure. The temperature dependent cladding thinning phenomenon was used for kinetic interpretation. As the first of a two-part study, this paper will focus on the exposure capsule performance, including metallographic cross-section preparation and preliminary results on the interface chemistry.
Pyriproxyfen Contamination in Daphnia magna: Identifying Early Warning Biomarkers
Authors: Salesa Beatriz , Torres-Gavilá Javier , Ferrando-Rodrigo María Dolores , Sancho Encarnación
Published: February 2024

Pyriproxyfen is an insecticide currently employed in numerous countries for the management of agricultural and indoor pests. Several studies indicate that this insecticide has been detected in multiple rivers, with concentrations reaching as high as 99.59 ng/L in the Júcar River in Spain. Therefore, the determination of some biochemical and genetic effects of this insecticide on aquatic organisms could serve as an early warning mechanism to identify potential disruptions in various biomarkers. Based on this, Daphnia magna organisms were exposed to pyriproxyfen sublethal concentrations for 21 days. Some biochemical parameters, including cholesterol, triglycerides, glucose, lactate, and LDH activity, were determined. Additionally, some genetic biomarkers associated with oxidative stress, heat shock proteins, lipid metabolism, hemoglobin, metallothioneins, and vitellogenin synthesis were evaluated in daphnids exposed to the insecticide for 21 days. LDH activity increased significantly in those daphnids exposed to the highest insecticide concentration (14.02 µg/L), while cholesterol levels decreased significantly. In contrast, glucose, total proteins, and triglycerides remained unaffected in D. magna exposed to pyriproxyfen. On the other hand, exposure to the insecticide led to notable alterations in gene expression among individuals. Specifically, genes associated with lipid metabolism and reproduction exhibited a significant reduction in gene expression. Fabd expression was decreased by approximately 20% in exposed daphnids, while vtg expression was suppressed as much as 80% when compared to control values. Furthermore, it was observed that the hgb1 and hgb2 genes, associated with hemoglobin synthesis, exhibited significant overexpression. Notably, the dysfunction observed in both hemoglobin genes was linked to an increase in pigmentation in Daphnia magna during the course of the experiment. These alterations in gene expression could serve as effective indicators of early contamination even at low pesticide concentrations.
The Integration of 3D Virtual Reality and 3D Printing Technology as Innovative Approaches to Preoperative Planning in Neuro-Oncology
Authors: González-López Pablo , Kuptsov Artem , Gómez-Revuelta Cristina , Fernández-Villa Jaime , Abarca-Olivas Javier , Daniel Roy T. , Meling Torstein R. , Nieto-Navarro Juan
Published: February 2024

Our study explores the integration of three-dimensional (3D) virtual reality (VR) and 3D printing in neurosurgical preoperative planning. Traditionally, surgeons relied on two-dimensional (2D) imaging for complex neuroanatomy analyses, requiring significant mental visualization. Fortunately, nowadays advanced technology enables the creation of detailed 3D models from patient scans, utilizing different software. Afterwards, these models can be experienced through VR systems, offering comprehensive preoperative rehearsal opportunities. Additionally, 3D models can be 3D printed for hands-on training, therefore enhancing surgical preparedness. This technological integration transforms the paradigm of neurosurgical planning, ensuring safer procedures.
A 20 MHz Repetition Rate, Sub-Picosecond Ti–Sapphire Laser for Fiber Delivery in Nonlinear Microscopy of the Skin
Authors: Krolopp Ádám , Fésűs Luca , Szipőcs Gergely , Wikonkál Norbert , Szipőcs Róbert
Published: February 2024

Nonlinear microscopy (NM) enables us to investigate the morphology or monitor the physiological processes of the skin through the use of ultrafast lasers. Fiber (or fiber-coupled) lasers are of great interest because they can easily be combined with a handheld, scanning nonlinear microscope. This latter feature greatly increases the utility of NM for pre-clinical applications and in vivo tissue imaging. Here, we present a fiber-coupled, sub-ps Ti–sapphire laser system being optimized for in vivo, stain-free, 3D imaging of skin alterations with a low thermal load of the skin. The laser is pumped by a low-cost, 2.1 W, 532 nm pump laser and delivers 0.5–1 ps, high-peak-power pulses at a ~20 MHz repetition rate. The spectral bandwidth of the laser is below 2 nm, which results in a low sensitivity for dispersion during fiber delivery. The reduction in the peak intensity due to the increased pulse duration is compensated by the lower repetition rate of our laser. In our proof-of-concept imaging experiments, a ~1.8 m long, commercial hollow-core photonic bandgap fiber was used for fiber delivery. Fresh and frozen skin biopsies of different skin alterations (e.g., adult hemangioma, basal cell cancer) and an unaffected control were used for high-quality, two-photon excitation fluorescence microscopy (2PEF) and second-harmonic generation (SHG) z-stack (3D) imaging.
Inverse Spectrum and Structure of Topological Metagroups
Authors: Ludkowski Sergey Victor
Published: February 2024

In this article, a structure of topological metagroups is scrutinized. Their inverse spectra are studied. This also permits us to construct abundant families of topological metagroups and quasigroups. Specific features of the topological quasigroups structure are found in comparison with topological groups, and are discussed.
Correction: Alohali et al. Ricci Vector Fields Revisited. Mathematics 2024, 12, 144
Authors: Alohali Hanan , Deshmukh Sharief , Vîlcu Gabriel-Eduard
Published: February 2024

In the original paper [...]
A Measure for the Vulnerability of Uniform Hypergraph Networks: Scattering Number
Authors: Zhao Ning , Zhao Haixing , Li Yinkui
Published: February 2024

The scattering number of a graph G is defined as s(G)=max{ω(G−X)−|X|:X⊂V(G),ω(G−X)>1}, where X is a cut set of G, and ω(G−X) denotes the number of components in G−X, which can be used to measure the vulnerability of network G. In this paper, we generalize this parameter to a hypergraph to measure the vulnerability of uniform hypergraph networks. Firstly, some bounds on the scattering number are given. Secondly, the relations of scattering number between a complete k-uniform hypergraph and complete bipartite k-uniform hypergraph are discussed.
Reversible Data Hiding for Color Images Using Channel Reference Mapping and Adaptive Pixel Prediction
Authors: He Dan , Cai Zhanchuan
Published: February 2024

Reversible data hiding (RDH) is a technique that embeds secret data into digital media while preserving the integrity of the original media and the secret data. RDH has a wide range of application scenarios in industrial image processing, such as intellectual property protection and data integrity verification. However, with the increasing prevalence of color images in industrial applications, traditional RDH methods for grayscale images are inadequate to meet the requirements of image fidelity. This paper proposes an RDH method for color images based on channel reference mapping (CRM) and adaptive pixel prediction. Initially, the CRM mode for a color image is established based on the pixel variation correlation between the RGB channels. Then, the pixel local complexity context is adaptively selected using the CRM mode. Next, each pixel value is adaptively predicted based on the features and characteristics of adjacent pixels and reference channels, and then data is embedded by expanding the prediction error. Finally, we compare seven existing RDH algorithms on the standard image dataset and the Kodak dataset to validate the advantages of our method. The experimental results demonstrate that our approach achieves average peak signal-to-noise ratio (PSNR) values of 63.61 and 60.53 dB when embedding 20,000 and 40,000 bits of data, respectively. These PSNR values surpass those of other RDH methods. These findings indicate that our method can effectively preserve the visual quality of images even under high embedding capacities.
Traffic Safety Assessment and Injury Severity Analysis for Undivided Two-Way Highway–Rail Grade Crossings
Authors: Ren Qiaoqiao , Xu Min , Zhou Bojian , Chung Sai-Ho
Published: February 2024

The safety and reliability of undivided two-way highway–rail grade crossings (HRGCs) are of paramount importance in transportation systems. Utilizing crash data from the Federal Railroad Administration between 2020 and 2021, this study aims to predict crash injury severity outcomes and investigate various factors influencing injury severities. The χ2 test was first used to select variables that were significantly associated with injury outcomes. By employing the eXtreme Gradient Boosting (XGBoost) model and interpretable SHapley Additive exPlanations (SHAP), a cross-category safety assessment that offers an evidence-based hierarchy and statistical inference of risk factors associated with crashes, crossings, vehicles, drivers, and environment was provided for killed, injured, and uninjured outcomes. Some significant predictors overlapped between the killed and injured models, such as old driver, driver was in vehicle, main track, went around the gate, adverse crossing surface, and truck, while the other different significant factors revealed that the model could distinguish between different severity levels. Additionally, the results suggested that the model has varying performances in predicting different injury severities, with the killed model having the highest accuracy of 93.36%. The SHAP dependency plots for the top three features also ensure reliable predictions and inform potential interventions aimed at strengthening traffic safety and risk management practices, such as enhanced warning systems and targeted educational campaigns for older drivers.
Deep Neural Network-Oriented Indicator Method for Inverse Scattering Problems Using Partial Data
Authors: Lin Yule , Yan Xiaoyi , Sun Jiguang , Liu Juan
Published: February 2024

We consider the inverse scattering problem to reconstruct an obstacle using partial far-field data due to one incident wave. A simple indicator function, which is negative inside the obstacle and positive outside of it, is constructed and then learned using a deep neural network (DNN). The method is easy to implement and effective as demonstrated by numerical examples. Rather than developing sophisticated network structures for the classical inverse operators, we reformulate the inverse problem as a suitable operator such that standard DNNs can learn it well. The idea of the DNN-oriented indicator method can be generalized to treat other partial data inverse problems.
Portable Dynamic Chest Radiography: Literature Review and Potential Bedside Applications
Authors: Cè Maurizio , Oliva Giancarlo , Rabaiotti Francesca Lucrezia , Macrì Laura , Zollo Sharon , Aquila Alessandro , Cellina Michaela
Published: February 2024

Dynamic digital radiography (DDR) is a high-resolution radiographic imaging technique using pulsed X-ray emission to acquire a multiframe cine-loop of the target anatomical area. The first DDR technology was orthostatic chest acquisitions, but new portable equipment that can be positioned at the patient’s bedside was recently released, significantly expanding its potential applications, particularly in chest examination. It provides anatomical and functional information on the motion of different anatomical structures, such as the lungs, pleura, rib cage, and trachea. Native images can be further analyzed with dedicated post-processing software to extract quantitative parameters, including diaphragm motility, automatically projected lung area and area changing rate, a colorimetric map of the signal value change related to respiration and motility, and lung perfusion. The dynamic diagnostic information along with the significant advantages of this technique in terms of portability, versatility, and cost-effectiveness represents a potential game changer for radiological diagnosis and monitoring at the patient’s bedside. DDR has several applications in daily clinical practice, and in this narrative review, we will focus on chest imaging, which is the main application explored to date in the literature. However, studies are still needed to understand deeply the clinical impact of this method.
Breaking Dynamic Behavior in 3D Covalent Organic Framework with Pre-Locked Linker Strategy
Authors: Chen Xiaohong , Yu Chengyang , Yusran Yusran , Qiu Shilun , Fang Qianrong
Published: February 2024

Due to their large surface area and pore volume, three-dimensional covalent organic frameworks (3D COFs) have emerged as competitive porous materials. However, structural dynamic behavior, often observed in imine-linked 3D COFs, could potentially unlock their potential application in gas storage. Herein, we showed how a pre-locked linker strategy introduces breaking dynamic behavior in 3D COFs. A predesigned planar linker-based 3,8-diamino-6-phenylphenanthridine (DPP) was prepared to produce non-dynamic 3D JUC-595, as the benzylideneamine moiety in DPP locked the linker flexibility and restricted the molecular bond rotation of the imine linkages. Upon solvent inclusion and release, the PXRD profile of JUC-595 remained intake, while JUC-594 with a flexible benzidine linker experienced crystal transformation due to framework contraction–expansion. As a result, the activated JUC-595 achieved higher surface areas (754 m2 g−1) than that of JUC-594 (548 m2 g−1). Furthermore, improved CO2 and CH4 storages were also seen in JUC-595 compared with JUC-594. Impressively, JUC-595 recorded a high normalized H2 storage capacity that surpassed other reported high-surface area 3D COFs. This works shows important insights on manipulating the structural properties of 3D COF to tune gas storage performance.
Cigarette Smoking as a Risk Factor for Tuberculosis in Adults: Epidemiology and Aspects of Disease Pathogenesis
Authors: Feldman Charles , Theron Annette J. , Cholo Moloko C. , Anderson Ronald
Published: February 2024

It has been noted by the World Health Organisation that cases of tuberculosis in 2022 globally numbered 10.6 million, resulting in 1.3 million deaths, such that TB is one of the infectious diseases causing the greatest morbidity and mortality worldwide. Since as early as 1918, there has been an ongoing debate as to the relationship between cigarette smoking and TB. However, numerous epidemiological studies, as well as meta-analyses, have indicated that both active and passive smoking are independent risk factors for TB infection, development of reactivation TB, progression of primary TB, increased severity of cavitary disease, and death from TB, among several other considerations. With this considerable body of evidence confirming the association between smoking and TB, it is not surprising that TB control programmes represent a key potential preventative intervention. In addition to coverage of the epidemiology of TB and its compelling causative link with smoking, the current review is also focused on evidence derived from clinical- and laboratory-based studies of disease pathogenesis, most prominently the protective anti-mycobacterial mechanisms of the alveolar macrophage, the primary intracellular refuge of M. tuberculosis. This section of the review is followed by an overview of the major strategies utilised by the pathogen to subvert these antimicrobial mechanisms in the airway, which are intensified by the suppressive effects of smoke inhalation on alveolar macrophage function. Finally, consideration is given to a somewhat under-explored, pro-infective activity of cigarette smoking, namely augmentation of antibiotic resistance due to direct effects of smoke per se on the pathogen. These include biofilm formation, induction of cellular efflux pumps, which eliminate both smoke-derived toxicants and antibiotics, as well as gene modifications that underpin antibiotic resistance.
Helicoid Morphology of Arthrospira platensis NIES-39 Confers Temperature Compensation in the Longitudinal Movement Velocity of Its Trichomes
Authors: Shiraishi Hideaki , Sasase Mari , Nakashima Ayano Sakaida
Published: February 2024

The velocity of the gliding movement of filamentous cyanobacteria on a solid surface usually has a strong temperature dependency, and the higher the temperature, the faster the speed. Former studies on this phenomenon were conducted using filamentous cyanobacteria with straight morphology. We examined the velocity of the gliding movement of Arthrospira platensis NIES-39 along its longitudinal axis to see if the same was true for this cyanobacterium with helicoid trichomes. Experimental results showed little temperature dependency in the velocity in a wide temperature range in this cyanobacterium. However, when we examined the velocity using mutants with straight trichomes, their velocity was strongly affected by temperature, like other formerly analyzed filamentous cyanobacteria. This result indicates that the helicoid morphology of A. platensis trichomes confers temperature compensation to their migration velocity, enabling them to keep a relatively constant velocity under various temperatures. Migration of wild-type trichomes is considerably suppressed compared to the straight-trichome mutants on solid media. The temperature compensation in the locomotion of this organism appears to be established as part of such a suppression. It was also found that the velocity of this cyanobacterium depended on the trichome length when they were atypically short (<250 µm); the shorter the trichomes, the slower the gliding movement tended to be. This result indicates that the coordinated action of a high number of cells constituting the trichome is required for efficient gliding movement.
Reciprocal Effects of Metal Mixtures on Phytoplankton
Authors: Nawaz Ammara , Šotek Pavlína Eliška , Molnárová Marianna
Published: February 2024

Several types of contaminants are anthropogenically introduced into natural aquatic ecosystems and interact with other chemicals and/or with living organisms. Although metal toxicity alone has been relatively well studied, the toxic metal ion effects in the mixture have been thoroughly studied only during the last decades. This review focuses on the published reciprocal effects of different metals on different species of algae, together with describing their toxic effects on studied parameters. Phytoplankton as a bioindicator can help to estimate the reciprocal metal risk factor. Many methodologies have been developed and explored, such as the biotic ligand model (BLM), concentration addition (CA), independent action (IA), sensitivity distribution of EC50 species sensitivity distribution (SSD curves), and others, to study reciprocal metal toxicity and provide promising results, which are briefly mentioned too. From our review, we can commonly conclude the following: Zn acted antagonistically with most heavy metals (Al, Cu, Cd, and Ni). The Cu interaction with Cd, Fe, and Pb was mostly antagonistic. Cd showed synergistic behaviour with Hg, Cu, Zn, and Pb and antagonistic behaviour with Co and Fe in many cases. Methods and techniques need to be developed and optimised to determine reciprocal metal toxicity so that the ecotoxicological predictions made by using phytoplankton can be more accurate and related to real-time toxic metals risks to the aquatic ecosystem. This is the main objective of ecotoxicological tests for risk assessment. Understanding how metals enter algal cells and organelles can help to solve this challenge and was one of the main parts of the review.
Characterization of OsPIN2 Mutants Reveal Novel Roles for Reactive Oxygen Species in Modulating Not Only Root Gravitropism but Also Hypoxia Tolerance in Rice Seedlings
Authors: Hao Bowen , Zhang Ruihan , Zhang Chengwei , Wen Na , Xia Yu , Zhao Yang , Li Qinying , Qiao Lei , Li Wenqiang
Published: February 2024

Tolerance to submergence-induced hypoxia is an important agronomic trait especially for crops in lowland and flooding-affected areas. Although rice (Oryza sativa) is considered a flood-tolerant crop, only limited cultivars display strong tolerance to prolonged submergence and/or hypoxic stress. Therefore, characterization of hypoxic resistant genes and/or germplasms have important theoretical and practical significance for rice breeding and sustained improvements. Previous investigations have demonstrated that loss-of-function of OsPIN2, a gene encoding an auxin efflux transporter, results in the loss of root gravitropism due to disrupted auxin transport in the root tip. In this study, we revealed a novel connection between OsPIN2 and reactive oxygen species (ROS) in modulating root gravitropism and hypoxia tolerance in rice. It is shown that the OsPIN2 mutant had decreased accumulation of ROS in root tip, due to the downregulation of glycolate oxidase encoding gene OsGOX6, one of the main H2O2 sources. The morphological defects of root including waved rooting and agravitropism in OsPIN2 mutant may be rescued partly by exogenous application of H2O2. The OsPIN2 mutant exhibited increased resistance to ROS toxicity in roots due to treatment with H2O2. Furthermore, it is shown that the OsPIN2 mutant had increased tolerance to hypoxic stress accompanied by lower ROS accumulation in roots, because the hypoxia stress led to over production of ROS in the roots of the wild type but not in that of OsPIN2 mutant. Accordingly, the anoxic resistance-related gene SUB1B showed differential expression in the root of the WT and OsPIN2 mutant in response to hypoxic conditions. Notably, compared with the wild type, the OsPIN2 mutant displayed a different pattern of auxin distribution in the root under hypoxia stress. It was shown that hypoxia stress caused a significant increase in auxin distribution in the root tip of the WT but not in that of the war1 mutant. In summary, these results suggested that OsPIN2 may play a role in regulating ROS accumulation probably via mediating auxin transport and distribution in the root tip, affecting root gravitropism and hypoxic tolerance in rice seedlings. These findings may contribute to the genetic improvement and identification of potential hypoxic tolerant lines in rice.
Functional and Transcriptome Analysis Reveal Specific Roles of Dimocarpus longan DlRan3A and DlRan3B in Root Hair Development, Reproductive Growth, and Stress Tolerance
Authors: Tian Qilin , Xie Xiying , Lai Ruilian , Cheng Chunzhen , Zhang Zihao , Chen Yukun , XuHan Xu , Lin Yuling , Lai Zhongxiong
Published: February 2024

Ran GTPases play essential roles in plant growth and development. Our previous studies revealed the nuclear localization of DlRan3A and DlRan3B proteins and proposed their functional redundancy and distinction in Dimocarpus longan somatic embryogenesis, hormone, and abiotic stress responses. To further explore the possible roles of DlRan3A and DlRan3B, gene expression analysis by qPCR showed that their transcripts were both more abundant in the early embryo and pulp in longan. Heterologous expression of DlRan3A driven by its own previously cloned promoter led to stunted growth, increased root hair density, abnormal fruits, bigger seeds, and enhanced abiotic stress tolerance. Conversely, constitutive promoter CaMV 35S (35S)-driven expression of DlRan3A, 35S, or DlRan3B promoter-controlled expression of DlRan3B did not induce the alterations in growth phenotype, while they rendered different hypersensitivities to abiotic stresses. Based on the transcriptome profiling of longan Ran overexpression in tobacco plants, we propose new mechanisms of the Ran-mediated regulation of genes associated with cell wall biosynthesis and expansion. Also, the transgenic plants expressing DlRan3A or DlRan3B genes controlled by 35S or by their own promoter all exhibited altered mRNA levels of stress-related and transcription factor genes. Moreover, DlRan3A overexpressors were more tolerant to salinity, osmotic, and heat stresses, accompanied by upregulation of oxidation-related genes, possibly involving the Ran-RBOH-CIPK network. Analysis of a subset of selected genes from the Ran transcriptome identified possible cold stress-related roles of brassinosteroid (BR)-responsive genes. The marked presence of genes related to cell wall biosynthesis and expansion, hormone, and defense responses highlighted their close regulatory association with Ran.
Shape Analysis of Prosthetic Socket Rectification Procedure for Transtibial Amputees
Authors: Nagarajan Yogeshvaran R. , Farukh Farukh , Silberschmidt Vadim V. , Kandan Karthikeyan , Singh Amit Kumar , Mukul Pooja
Published: February 2024

Achieving a comfortable socket residual limb interface is crucial for effective prosthetic rehabilitation, depending on the precise characterisation and fluctuations in the shape and volume of residual limbs. Clinicians rely on subjective and iterative methods for shaping sockets, often involving a trial-and-error approach. This study introduces a framework for measuring, analysing, and comparing residual limb shape and volume using scanned data to facilitate more informed clinical decision-making. Surface scans of 44 transtibial residual limb casts of various sizes and lengths were examined. All scans were spatially aligned to a mid-patella and subjected to analysis using a shape analysis toolbox. Geometric measurements were extracted, with particular attention to significant rectified regions during the cast rectification process. Following PTB guidelines, our analysis revealed substantial alterations, primarily in the mid-patella region, followed by the patellar tendon area. Notably, there was a significant volume change of 6.02% in the region spanning from mid-patella to 25% of the cast length. Beyond this point, linear cast modifications were observed for most amputees up to 60% of the cast length, followed by individual-specific deviations beyond this region. Regardless of residual limb size and length, the modifications applied to positive casts suggested categorising patients into five major groups. This study employs the AmpScan shape analysis tool, to comprehend the cast rectification process used for capturing and assessing the extent of rectification on patients’ residual limb casts. The clinical implications of our research are threefold: (a) the comparison data can serve as training resources for junior prosthetists; (b) this will aid prosthetists in identifying specific regions for rectification and assessing socket fit; (c) it will help in determining optimal timing for prosthetic fitting or replacement.
In Mind and Spirit: The Psychosocial Impacts of Religiosity in Youth Mental Health Treatment
Authors: Klee Katherine , Bartkowski John P.
Published: February 2024

The rise in suicides among elementary- to high-school-aged youth has alarmed health professionals for years, only to be amplified by the long-lasting effects of the COVID-19 pandemic. Religion and spirituality offer many people significant psychosocial support in pandemic circumstances, often acting as platforms for hope and social connectedness. Yet, given the adultocentric world they inhabit, young people must often negotiate or reconsider the role of religion and spirituality in the context of their developmental trajectory. This research explores mental health professionals’ approaches to religiosity and spirituality in the delivery of therapeutic care to youth at risk of suicide. Qualitative analyses of interview transcripts conducted with youth mental health clinicians in the state of Texas underscore a myriad of contextual factors related to treating suicidal ideation and behaviors. We categorize our findings according to licensed mental health professionals’ (1) navigation of youth clients’ religious/spiritual preferences aligned with or opposed to familial preferences; (2) selective integration of youth-oriented religious/spiritual elements into treatment as warranted; and (3) reflections on the impacts of religion/spirituality on treatment efficacy for child and adolescent clients. This study adds to current research on religion and spirituality’s impact on mental health and its therapeutic integration into treatment practices tailored for youth.
Geopolymerization of Recycled Glass Waste: A Sustainable Solution for a Lightweight and Fire-Resistant Material
Authors: Valanides Marios , Aivaliotis Konstantinos , Oikonomopoulou Konstantina , Fikardos Alexandros , Savva Pericles , Sakkas Konstantinos , Nicolaides Demetris
Published: February 2024

Glass is considered a sustainable material with achievable recovery rates within the EU. However, there are limited data available for construction glass waste management. Furthermore, glass is a heavy material, and considering the geographical limitations of Cyprus, the transportation trading cost within the EU is extremely high. Therefore, another method for utilizing this by-product should be developed. The aim of this research is to investigate the production of a low-cost, lightweight and fireproof material able to retain its structural integrity, using the geopolymerization method with the incorporation of randomly collected construction glass waste. The glass waste was initially processed in a Los Angeles abrasion machine and then through a Micro-Deval apparatus in order to be converted to a fine powder. Mechanical (compressive and flexural strength), physical (setting time and water absorption) and thermal properties (thermal conductivity) were investigated. The fire-resistant materials presented densities averaging 450 kg/m3 with a range of compressive strengths of 0.5 to 3 MPa. Additionally, a techno-economic analysis was conducted to evaluate the viability of the adopted material. Based on the results, the final geopolymer product has the potential to be utilized as a fire resistance material, preventing yielding or spalling.
A New Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method to Monitor 1-Amino-4-Methyl-Piperazine and 1-Methyl-4-Nitroso-Piperazine in Rifampicin Hydrolysis: A Standard Addition Approach to Quantify Nitrosamine in Rifampicin
Authors: Panusa Alessia , Rotundo Paola , Sadutto Daniele , Rodomonte Andrea , Cirilli Roberto
Published: February 2024

1-amino-4-methyl-piperazine (AMP) is both a starting material for the synthesis of rifampicin (RIF) and a degradation product of RIF hydrolysis. 1-methyl-4-nitroso-piperazine (MNP) is an oxidation product of AMP as well as a potentially genotoxic N-nitrosamine. The EMA and FDA have approved an ad interim limit of 5 ppm for MNP in RIF drug products. As in-house methods for the analysis of MNP in RIF use a wide range of conditions for mobile phases and sample diluents, we decided to investigate whether these conditions affect the formation of MNP and AMP. A UHPLC-MS/MS method was developed to simultaneously quantify AMP and MNP during RIF hydrolysis in buffered aqueous solutions at different pH levels. Analyses were performed in MRM mode; separations were carried out on an InfinityLab Poroshell HPH-C18 (100 mm, 2.1 mm i.d., particle size 1.9 μm). In aqueous RIF solutions, the content of AMP and MNP increases with time; at different pHs, the concentration of AMP increases much faster in acidic than in basic solutions; and the increase in MNP can be reduced by the addition of ascorbic acid. To avoid an overestimation of MNP, water should not be used as a diluent in RIF sample preparations. Methanol is a more suitable diluent than water. A standard addition method has been validated for the quantification of MNP in RIF drug substances.
An Artificial Intelligence and Industrial Internet of Things-Based Framework for Sustainable Hydropower Plant Operations
Authors: Fera Fation T. , Spandonidis Christos
Published: February 2024

Hydropower plays a crucial role in supplying electricity to developed nations and is projected to expand its capacity in various developing countries such as Sub-Saharan Africa, Argentina, Colombia, and Turkey. With the increasing demand for sustainable energy and the emphasis on reducing carbon emissions, the significance of hydropower plants is growing. Nevertheless, numerous challenges arise for these plants due to their aging infrastructure, impacting both their efficiency and structural stability. In order to tackle these issues, the present study has formulated a specialized real-time framework for identifying damage, with a particular focus on detecting corrosion in the conductors of generators within hydropower plants. It should be noted that corrosion processes can be highly complex and nonlinear, making it challenging to develop accurate physics-based models that capture all the nuances. Therefore, the proposed framework leverages autoencoder, an unsupervised, data-driven AI technology with the Mahalanobis distance, to capture the intricacies of corrosion and automate its detection. Rigorous testing shows that it can identify slight variations indicating conductor corrosion with over 80% sensitivity and a 5% false alarm rate for ‘medium’ to ‘high’ severity damage. By detecting and resolving corrosion early, the system reduces disruptions, streamlines maintenance, and mitigates unscheduled repairs’ negative effects on the environment. This enhances energy generation effectiveness, promotes hydroelectric facilities’ long-term viability, and fosters community prosperity.
Genomic Insights into the Taxonomy and Metabolism of the Cyanobacterium Pannus brasiliensis CCIBt3594
Authors: Machado Mauricio Junior , Botero Natalia Betancurt , Andreote Ana Paula Dini , Feitosa Anderson Miguel Teixeira , Popin Rafael Vicentini , Sivonen Kaarina , Fiore Marli F.
Published: February 2024

The freshwater Pannus genus comprises cyanobacterial unicellular species with a particular morphology, forming free-floating rounded colonies with thin, homogenous, and colorless colonial mucilage. There is little literature on the taxonomy of the Pannus and none on its metabolism. This study presents the first genomic characterization of a Pannus strain isolated from Pantanal Biome, Brazil. The genome was assembled into 117 contigs with a total size of 5.1 Mb and 99.12% completeness. It contained 4988 protein-encoding genes, including some involved in secondary metabolite biosynthesis, such as cyanobactin and terpenes. Interestingly, P. brasiliensis CCIBt3594 has a complete set of nitrogen fixation genes and is a non-heterocytou unicellular cyanobacterium. Finally, the phylogenomic analyses revealed the lack of information on closely related strains and anchored the genus Pannus within the order Chroococcales, Microcystaceae family, closest to Microcystis spp. representatives. This work presents novel evidence concerning a sparsely characterized genus of the Cyanobacteria phylum and contributes to elucidating taxonomic and systematic issues within the group of unicellular cyanobacteria.
The Influence of COVID-19 on Patient Mobilization and Injury Attributes in the ICU: A Retrospective Analysis of a Level II Trauma Center
Authors: Navarro Yelissa , Huang Elizabeth , Johnson Chandler , Clark Forrest , Coppola Samuel , Modi Suraj , Warren Gordon L. , Call Jarrod A.
Published: February 2024

The objectives of this study were to determine the effect of COVID-19 on physical therapy (PT) mobilization of trauma patients and to determine if mobilization affected patient course in the ICU. This retrospective study included patients who were admitted to the ICU of a level II trauma center. The patients were divided into two groups, i.e., those admitted before (n = 378) and after (n = 499) 1 April 2020 when Georgia’s COVID-19 shelter-in-place order was mandated. The two groups were contrasted on nominal and ratio variables using Chi-square and Student’s t-tests. A secondary analysis focused specifically on the after-COVID patients examined the extent to which mobilization (n = 328) or lack of mobilization (n = 171) influenced ICU outcomes (e.g., mortality, readmission). The two groups were contrasted on nominal and ratio variables using Chi-square and Student’s t-tests. The after-COVID patients had higher injury severity as a greater proportion was classified as severely injured (i.e., >15 on Injury Severity Score) compared to the before-COVID patients. After-COVID patients also had a greater cumulative number of comorbidities and experienced greater complications in the ICU. Despite this, there was no difference between patients in receiving a PT consultation or days to mobilization. Within the after-COVID cohort, those who were mobilized were older, had greater Glasgow Coma Scale scores, had longer total hospital days, and had a lesser mortality rate, and a higher proportion were female. Despite shifting patient injury attributes post-COVID-19, a communicable disease, mobilization care remained consistent and effective.