bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Analysis of HIBM Myopathy-Specific GNE V727M Hotspot Mutation Identifies a Novel COL6A3 Allied Gene Signature That Is Also Deregulated in Multiple Neuromuscular Diseases and Myopathies
Authors: Attri Shivangi , Lone Moien , Katiyar Amit , Sharma Vikas , Kumar Vinay , Verma Chaitenya , Gahlawat Suresh Kumar
Published: February 2023

The GNE-associated V727M mutation is one of the most prevalent ethnic founder mutations in the Asian HIBM cohort; however, its role in inducing disease phenotype remains largely elusive. In this study, the function of this hotspot mutation was profoundly investigated. For this, V727M mutation-specific altered expression profile and potential networks were explored. The relevant muscular disorder-specific in vivo studies and patient data were further analyzed, and the key altered molecular pathways were identified. Our study found that the GNEV727M mutation resulted in a deregulated lincRNA profile, the majority of which (91%) were associated with a down-regulation trend. Further, in silico analysis of associated targets showed their active role in regulating Wnt, TGF-β, and apoptotic signaling. Interestingly, COL6a3 was found as a key target of these lincRNAs. Further, GSEA analysis showed HIBM patients with variable COL6A3 transcript levels have significant alteration in many critical pathways, including epithelial-mesenchymal-transition, myogenesis, and apoptotic signaling. Interestingly, 12 of the COL6A3 coexpressed genes also showed a similar altered expression profile in HIBM. A similar altered trend in COL6A3 and coexpressed genes were found in in vivo HIBM disease models as well as in multiple other skeletal disorders. Thus, the COL6A3-specific 13 gene signature seems to be altered in multiple muscular disorders. Such deregulation could play a pivotal role in regulating many critical processes such as extracellular matrix organization, cell adhesion, and skeletal muscle development. Thus, investigating this novel COL6A3-specific 13 gene signature provides valuable information for understanding the molecular cause of HIBM and may also pave the way for better diagnosis and effective therapeutic strategies for many muscular disorders.
Detecting Embryo Developmental Potential by Single Blastomere RNA-Seq
Authors: Nõmm Monika , Ivask Marilin , Pärn Pille , Reimann Ene , Kõks Sulev , Jaakma Ülle
Published: February 2023

Recent advances in preimplantation embryo diagnostics enable a wide range of applications using single cell biopsy and molecular-based selection techniques without compromising embryo production. This study was conducted to develop a single cell embryo biopsy technique and gene expression analysis method with a very low input volume to ensure normal embryo development and to see if there are differences in gene expression profiles between day-5 biopsied bovine embryos that developed into blastocysts and embryos arrested at morula stage. Out of the 65 biopsied morulae, 32 developed to blastocysts (49.2%). Out of the 13,580 successfully annotated genes, 1204 showed a difference in mRNA expression level. Out of these, 155 genes were expressed in embryos developing to blastocysts. The pathway enrichment analysis revealed significant enrichment in “organelle biogenesis and maintenance”, “mRNA splicing” and “mitochondrial translation” pathways. These findings suggest principal differences in gene expression patterns and functional networks of embryos able to reach the blastocyst stage compared to embryos arrested in development. Our preliminary data suggest that single blastomere biopsy and selected gene expression profiles at morula stage could offer additional possibilities for early preimplantation embryo selection before transfer.
Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata
Authors: Wang Luhao , Qiao Feng , Geng Guigong , Lu Yueheng
Published: February 2023

Lamiophlomis rotata (Benth.) Kudo is a perennial and unique medicinal plant of the Qinghai–Tibet Plateau. It has the effects of diminishing inflammation, activating blood circulation, removing blood stasis, reducing swelling, and relieving pain. However, thus far, reliable reference gene identifications have not been reported in wild L. rotata. In this study, we identified suitable reference genes for the analysis of gene expression related to the medicinal compound synthesis in wild L. rotata subjected to five different-altitude habitats. Based on the RNA-Seq data of wild L. rotata from five different regions, the stability of 15 candidate internal reference genes was analyzed using geNorm, NormFinder, BestKeeper, and RefFinder. TFIIS, EF-1α, and CYP22 were the most suitable internal reference genes in the leaves of L. rotata from different regions, while OBP, TFIIS, and CYP22 were the optimal reference genes in the roots of L. rotata. The reference genes identified here would be very useful for gene expression studies with different tissues in L. rotata from different habitats.
An Entropy-Based Directed Random Walk for Cancer Classification Using Gene Expression Data Based on Bi-Random Walk on Two Separated Networks
Authors: Tay Xin Hui , Kasim Shahreen , Sutikno Tole , Fudzee Mohd Farhan Md , Hassan Rohayanti , Patah Akhir Emelia Akashah , Aziz Norshakirah , Seah Choon Sen
Published: February 2023

The integration of microarray technologies and machine learning methods has become popular in predicting the pathological condition of diseases and discovering risk genes. Traditional microarray analysis considers pathways as a simple gene set, treating all genes in the pathway identically while ignoring the pathway network’s structure information. This study proposed an entropy-based directed random walk (e-DRW) method to infer pathway activities. Two enhancements from the conventional DRW were conducted, which are (1) to increase the coverage of human pathway information by constructing two inputting networks for pathway activity inference, and (2) to enhance the gene-weighting method in DRW by incorporating correlation coefficient values and t-test statistic scores. To test the objectives, gene expression datasets were used as input datasets while the pathway datasets were used as reference datasets to build two directed graphs. The within-dataset experiments indicated that e-DRW method demonstrated robust and superior performance in terms of classification accuracy and robustness of the predicted risk-active pathways compared to the other methods. In conclusion, the results revealed that e-DRW not only improved the prediction performance, but also effectively extracted topologically important pathways and genes that were specifically related to the corresponding cancer types.
Feasibility of Community Pharmacist-Initiated and Point-of-Care CYP2C19 Genotype-Guided De-Escalation of Oral P2Y12 Inhibitors
Authors: Levens Amar D. , den Haan Melina C. , Jukema J. Wouter , Heringa Mette , van den Hout Wilbert B. , Moes Dirk Jan A. R. , Swen Jesse J.
Published: February 2023

Tailoring antiplatelet therapy based on CYP2C19 pharmacogenetic (PGx) testing can improve cardiovascular outcomes and potentially reduce healthcare costs in patients on a P2Y12-inhibitor regime with prasugrel or ticagrelor. However, ubiquitous adoption—particularly in an outpatient setting—remains limited. We conducted a proof-of-concept study to evaluate the feasibility of CYP2C19-guided de-escalation of prasugrel/ticagrelor to clopidogrel through point-of-care (POC) PGx testing in the community pharmacy. Multiple feasibility outcomes were assessed. Overall, 144 patients underwent CYP2C19 PGx testing in 27 community pharmacies. Successful test results were obtained in 142 patients (98.6%). De-escalation to clopidogrel occurred in 19 patients (20%) out of 95 (67%) eligible for therapy de-escalation, which was mainly due to PGx testing not being included in cardiology guidelines. Out of the 119 patients (84%) and 14 pharmacists (100%) surveyed, 109 patients (92%) found the community pharmacy a suitable location for PGx testing, and the majority of pharmacists (86%) thought it has added value. Net costs due to PGx testing were estimated at €43 per patient, which could be reduced by earlier testing and could turn into savings if de-escalation would double to 40%. Although the observed de-escalation rate was low, POC CYP2C19-guided de-escalation to clopidogrel appears feasible in a community pharmacy setting.
Genetic and Genomic Analysis of Cow Mortality in the Israeli Holstein Population
Authors: Weller Joel Ira , Ezra Ephraim , Seroussi Eyal , Gershoni Moran
Published: February 2023

“Livability” was defined as the inverse of the probability of death. The objectives of this study were to estimate the heritability, genetic and phenotypic trends for the livability of Israeli Holstein cows; estimate the genetic and environmental correlations between livability and the nine traits included in the Israeli breeding index; estimate the effect of the inclusion of livability in the Israeli breeding index on expected genetic gains; and compute a genome-wide association study (GWAS) for livability. Seven data sets were analyzed. All data were derived from the database of the Israeli dairy cattle herd-book. The mean livability for the complete data set of 523,954 cows born from 2000 through 2016 was 89.6%. Pregnancy reduced livability by 15%. Livability generally increased with parity and days in milk within parity. Heritability of livability was 0.0082. Phenotypic and genetic trends over the 14-year period from 2000 through 2013 were −0.42% and −0.22% per year. If livability is included in the Israeli breeding index, accounting for 9% of the index, livability would increase by 1.3% and protein production would decrease by 11 kg over the next decade, as compared to the current index. A marker in proximity to the oxytocin–vasopressin locus had the greatest effect in the GWAS. Oxytocin activity in cattle affects calving-associated pathologies and maternal death. Inclusion of livability in the Israeli breeding index is not recommended.
Distribution and Expression of Pulmonary Ionocyte-Related Factors CFTR, ATP6V0D2, and ATP6V1C2 in the Lungs of Yaks at Different Ages
Authors: He Junfeng , Wei Yating , Cui Yan , Zhang Qian
Published: February 2023

In order to reveal the distribution and expression characteristics of the pulmonary ionocyte-related factors CFTR, ATP6V0D2, and ATP6V1C2 in the lungs of yaks of different ages. Explore the possible regulation of these pulmonary ionocyte-related factors in the yak lung for adaptation to high-altitude hypoxia. The localization and expression of CTFR, ATP6V0D2, and ATP6V1C2 in the lungs of newborn, juvenile, adult, and elderly yaks were studied using immunohistochemistry, quantitative reverse transcription PCR, and Western blotting. Immunohistochemistry showed that CFTR, ATP6V0D2 and ATP6V1C2 were mainly localized in the ciliated cells and club cells of the epithelial mucosal layer of the bronchus and its branches in the lungs. For the qRT-PCR, expression of CFTR, ATP6V0D2 and ATP6V1C2 in the yak lungs varied according to age. For Western blotting, CFTR expression in the newborn group was significantly higher than in the other three groups. ATP6V0D2 expression of the adult group was significantly higher. ATP6V1C2 expression was the highest in the juvenile group (p < 0.05). This study showed that ciliated cells and club cells were related to the pulmonary ionocytes in yaks. CFTR, ATP6V0D2, and ATP6V1C2 were related to adaptations of yak lungs to high altitude hypoxia, through prevention of airway damage.
PCDH19 in Males: Are Hemizygous Variants Linked to Autism?
Authors: Chouery Eliane , Makhlouf Jana , Daoud Khatoun Wassim , Mehawej Cybel , Megarbane Andre
Published: February 2023

Background: Autism spectrum disorder (ASD) is a complex developmental disability that impairs the social communication and interaction of affected individuals and leads to restricted or repetitive behaviors or interests. ASD is genetically heterogeneous, with inheritable and de novo genetic variants in more than hundreds of genes contributing to the disease. However, these account for only around 20% of cases, while the molecular basis of the majority of cases remains unelucidated as of yet. Material and methods: Two unrelated Lebanese patients, a 7-year-old boy (patient A) and a 4-year-old boy (patient B), presenting with ASD were included in this study. Whole-exome sequencing (WES) was carried out for these patients to identify the molecular cause of their diseases. Results: WES analysis revealed hemizygous variants in PCDH19 (NM_001184880.1) as being the candidate causative variants: p.Arg787Leu was detected in patient A and p.Asp1024Asn in patient B. PCDH19, located on chromosome X, encodes a membrane glycoprotein belonging to the protocadherin family. Heterozygous PCDH19 variants have been linked to epilepsy in females with mental retardation (EFMR), while mosaic PCDH19 mutations in males are responsible for treatment-resistant epilepsy presenting similarly to EFMR, with some reported cases of comorbid intellectual disability and autism. Interestingly, a hemizygous PCDH19 variant affecting the same amino acid that is altered in patient A was previously reported in a male patient with ASD. Conclusion: Here, we report hemizygous PCDH19 variants in two males with autism without epilepsy. Reporting further PCDH19 variants in male patients with ASD is important to assess the possible involvement of this gene in autism.
PARK2 Microdeletion or Duplications Have Been Implicated in Different Neurological Disorders Including Early Onset Parkinson Disease
Authors: Ahmad Ausaf , Nkosi Dingani , Iqbal Mohammed A.
Published: February 2023

The PARK2 gene is located on 6q26, encodes ubiquitin-E3- ligase, and is a transcriptional repressor of p53. It contains 12 exons. PARK2 copy number variants has been reported in various types of neurodevelopmental disorders, namely schizophrenia, Parkinson’s disease (PD), autism spectrum disorder (ASD), and attention-deficit/hyperactivity disorder (ADHD). In this retrospective study, nine cases (five with microdeletion and four with microduplication) are reported with 6q26 deletion disrupting the PARK2 gene. Microdeletion sizes ranged between 215 Kb and 356 Kb, and duplication between 279 Kb and 726 Kb. These were present within the exons 7–10. Family follow up with FISH probes revealed paternal inheritance in two cases, maternal in two cases, and de novo origin in one case. Our results support previous studies showing that patients with PARK2 CNVs involving exons 5–12 might be more deleterious and cause a unique syndrome. Comprehensive analysis of additional case studies is needed to have a full characterization of this neurological disorder syndrome.
Hand Dexterity Is Associated with the Ability to Resolve Perceptual and Cognitive Interference in Older Adults: Pilot Study
Authors: Schwalbe Marie , Satz Skye , Miceli Rachel , Hu Hang , Manelis Anna
Published: February 2023

The relationship between hand dexterity and inhibitory control across the lifespan is underexplored. In this pilot study, we examined inhibitory control using a modified Simon task. During the task, participants were presented with right- and left-pointing arrows located either on the right or the left parts of the screen. In the congruent trials, the arrow location and direction matched. In the incongruent trials, they mismatched, thus creating cognitive interference. In 50% of trials, the arrow presentation was accompanied by a task-irrelevant but environmentally meaningful sound that created perceptual interference. Hand dexterity was measured with the 9-hole peg test. Significantly faster reaction time (RT) on the modified Simon task (p < 0.001) was observed in younger adults, trials with concurrent sound stimuli, and congruent trials. Older adults who reported recent falls had greater difficulty resolving cognitive interference than older adults without recent falls. Hand dexterity significantly moderated the effect of sound on RT, but only in the group of older individuals. Interestingly, older individuals with reduced hand dexterity benefited from concurrent sounds more than those with better hand dexterity. Our findings suggest that task-irrelevant but environmentally meaningful sounds may increase alertness and enhance stimulus perception and recognition, thus improving motor performance in older individuals.
A Summary of Current Guidelines and Future Directions for Medical Management and Monitoring of Patients with Cystinuria
Authors: Azer Sarah M. , Goldfarb David S.
Published: February 2023

Cystinuria is the most common genetic cause of recurrent kidney stones. As the result of a genetic defect in proximal tubular reabsorption of filtered cystine, increased urine levels of the poorly soluble amino acid result in recurrent cystine nephrolithiasis. Recurrent cystine stones not only adversely affect the quality of patients suffering from cystinuria but also may result in chronic kidney disease (CKD) from recurrent renal injury. Thus, the mainstay of medical management revolves around prevention of stones. Recently published consensus statements on guidelines for managing cystinuria were released from both the United States and Europe. The purpose of this review is to summarize guidelines for medical management of patients with cystinuria, to provide new insight into the utility and clinical significance of cystine capacity—an assay for monitoring cystinuria, and to discuss future directions for research on treatment of cystinuria. We discuss future directions, including the potential use of cystine mimetics, gene therapy, V2-receptor blockers, and SGLT2 inhibitors, topics which have not appeared in more recent reviews. It is notable that in the absence of randomized, controlled trials, the recommendations cited here and in the guidelines are based on our best understanding of the disorder’s pathophysiology, observational studies, and clinical experience.
Geographical Disparities in Esophageal Cancer Incidence and Mortality in the United States
Authors: Vedire Yeshwanth , Rana Navpreet , Groman Adrienne , Siromoni Beas , Yendamuri Sai , Mukherjee Sarbajit
Published: February 2023

Background: Our previous research on neuroendocrine and gastric cancers has shown that patients living in rural areas have worse outcomes than urban patients. This study aimed to investigate the geographic and sociodemographic disparities in esophageal cancer patients. Methods: We conducted a retrospective study on esophageal cancer patients between 1975 and 2016 using the Surveillance, Epidemiology, and End Results database. Both univariate and multivariable analyses were performed to evaluate overall survival (OS) and disease-specific survival (DSS) between patients residing in rural (RA) and urban (MA) areas. Further, we used the National Cancer Database to understand differences in various quality of care metrics based on residence. Results: N = 49,421 (RA [12%]; MA [88%]). The incidence and mortality rates were consistently higher during the study period in RA. Patients living in RA were more commonly males (p < 0.001), Caucasian (p < 0.001), and had adenocarcinoma (p < 0.001). Multivariable analysis showed that RA had worse OS (HR = 1.08; p < 0.01) and DSS (HR = 1.07; p < 0.01). Quality of care was similar, except RA patients were more likely to be treated at a community hospital (p < 0.001). Conclusions: Our study identified geographic disparities in esophageal cancer incidence and outcomes despite the similar quality of care. Future research is needed to understand and attenuate such disparities.
Convergent Validity of the Edinburgh Postnatal Depression Scale and the Patient Health Questionnaire (PHQ-9) in Pregnant and Postpartum Women: Their Construct Correlations with Functional Disability
Authors: Srisurapanont Manit , Oon-arom Awirut , Suradom Chawisa , Luewan Suchaya , Kawilapat Suttipong
Published: February 2023

This study aimed to evaluate the convergent validity of the Edinburgh Postnatal Depression Scale (EPDS) and the Patient Health Questionnaire (PHQ-9) in Thai pregnant and postpartum women, using the 12-item WHO Disability Assessment Schedule (WHODAS) as the reference standard. Participants completed the EPDS, PHQ-9, and WHODAS during the third trimester of pregnancy (over 28 weeks in gestational age) and six weeks postpartum. The sample included 186 and 136 participants for the antenatal and postpartum data analyses, respectively. The antenatal and postpartum data showed moderate correlations between both the EPDS and the PHQ-9 scores and the WHODAS scores (Spearman’s correlation coefficients = 0.53–0.66, p < 0.001). The EPDS and PHQ-9 were moderately accurate in distinguishing disability (WHODAS score ≥ 10) from non-disability (WHODAS score < 10) in pregnant and postpartum participants, but the area under the curve of the PHQ-9 receiver operating characteristic curves in postpartum participants was significantly larger than that of the EPDS, with a difference (95% CI; p-value) of 0.08 (0.16, 0.01; p = 0.044). In conclusion, the EPDS and PHQ-9 are valid for assessing PND-related disability in pregnant and postpartum women. The PHQ-9 may perform better than the EPDS in distinguishing disability from non-disability in postpartum women.
Overlooking of Individuals with Cardiometabolic Risk by Evaluation of Obesity Using Waist Circumference and Body Mass Index in Middle-Aged Japanese Women
Authors: Wakabayashi Ichiro
Published: February 2023

Waist circumference is often used for the diagnosis of visceral obesity and metabolic syndrome. In Japan, obesity in women is defined by the government as a waist circumference of ≥90 cm and/or BMI of ≥25 kg/m2. However, there has been a controversy for almost two decades as to whether waist circumference and its above-optimal cutoff are appropriate for the diagnosis of obesity in health checkups. Instead of waist circumference, the waist-to-height ratio has been recommended for the diagnosis of visceral obesity. In this study, the relationships between the waist-to-height ratio and cardiometabolic risk factors, including diabetes, hypertension and dyslipidemia, were investigated in middle-aged Japanese women (35~60 years) who were diagnosed as not having obesity according to the above Japanese criteria of obesity. The percentage of subjects showing normal waist circumference and normal BMI was 78.2%, and about one-fifth of those subjects (16.6% of the overall subjects) showed a high waist-to-height ratio. In subjects with normal waist circumference and normal BMI, odds ratios of high vs. not high waist-to-height ratio for diabetes, hypertension and dyslipidemia were significantly higher than the reference level. A considerable proportion of women who have a high cardiometabolic risk might be overlooked at annual lifestyle health checkups in Japan.
Effect of Sodium-Glucose Cotransporter 2 Inhibitors on Clinical and Laboratory Variables in Heart Failure Patients with Reduced Left Ventricular Ejection Fraction in a Latin American Hospital: A Retrospective Study
Authors: Speranza-Sánchez Mario Osvaldo , Díaz-Madriz José Pablo , Zavaleta-Monestel Esteban , Chaverri-Fernández José Miguel , Arguedas-Chacón Sebastián , Blanco-Jara Marleny , Fallas-Mora Abigail , Velásquez-Alfaro Luis Daniel
Published: February 2023

Heart failure (HF) is a syndrome suffered by more than 26 million people worldwide. SGLT2 inhibitors are drugs that have been shown to positively affect the management of HF patients, regardless of their diabetes status. A retrospective observational study was conducted on heart failure patients with reduced ejection fraction (HFrEF) enrolled at the HF clinic, who were on SGLT2 inhibitors. For these patients, baseline and follow-up data were collected and analyzed over time. Changes over time were quantified and statistical analysis was conducted to validate whether the changes were significant. After the screening of all the HF program patients, 24 met the inclusion criteria, with an average age of 68 years. Through the study, it was possible to find a statistically significant difference in the values of NT-ProBNP before and after adding a SGLT2 inhibitor in 14 patients (p = 0.0214). In addition, there was an improvement in the NYHA functional scale of 71% and no significant change in renal function or other laboratory values. Based on the studied parameters and throughout the clinical changes during the follow-up period, it was possible to establish an improvement in HFrEF patients on SGLT2 inhibitors as part of their therapy.
Visiting Heritage Sites in AR and VR
Authors: Pervolarakis Zacharias , Zidianakis Emmanouil , Katzourakis Antonis , Evdaimon Theodoros , Partarakis Nikolaos , Zabulis Xenophon , Stephanidis Constantine
Published: February 2023

Advances in digitization technologies have made possible the digitization of entire archaeological sites through a combination of technologies, including aerial photogrammetry, terrestrial photogrammetry, and terrestrial laser scanning. At the same time, the evolution of computer algorithms for data processing and the increased processing power made possible the combination of data from multiple scans to create a synthetic representation of large-scale sites. Finally, post-processing techniques and the evolution of computer and mobile GPUs and game engines have made possible the exploitation of digitization outcomes to further scientific study and historical preservation. This route was opened by the gaming industry. In terms of research, the exploitation of these new assets in conjunction with new visual rendering technologies, such as virtual and augmented reality, can create new dimensions for education and leisure. In this paper, we explore the usage of large-scale digitization of a heritage site to create a unique virtual visiting experience that can be accessed offline in VR and AR and on-site when visiting the archaeological site.
Ultra-Short Pulse Laser Cleaning of Contaminated Pleistocene Bone: A Comprehensive Study on the Influence of Pulse Duration and Wavelength
Authors: Rahman Md. Ashiqur , de la Fuente Germán F. , Miguel Carretero José , Abad Mª Pilar Alonso , Alcalde Rodrigo Alonso , Chapoulie Rémy , Schiavon Nick , Angurel Luis A.
Published: February 2023

The impact of wavelength and pulse duration in laser cleaning of hard blackish contaminants crust from archaeologically significant Pleistocene bone is investigated in this research. The objective is to determine the practical cleaning procedures and identify adequate laser parameters for cleaning archaeological bone from Sima de los Huesos (Spain) based on conservation and restoration perspectives. Bone surface cleaning was performed utilizing two Q-switched Nd:YAG lasers: sub-nanosecond pulsed lasers with emission wavelengths at 355 nm and 1064 nm, respectively, and a Yb:KGW femtosecond pulsed laser with an emission wavelength in the third harmonic at 343 nm. In all experiments, the laser beam scanning mode was applied to measure cleaning efficiency in removing contaminants and degradation products while assessing the underlying substrate surface damage. Several properties, including wavelength-dependent absorption, pulse repetition rate, and thermal properties of the material, are analyzed when evaluating the ability of these lasers to boost the cleaning efficiency of the deteriorated bone surface. Bone surface morphology and composition were studied and compared before and after laser irradiation, using Optical Microscopy, Scanning Electron Microscopy with Energy Dispersive X-ray Spectrometry (SEM-EDS), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Photoelectron Spectroscopy (XPS) characterization methods. The results indicate that 238-femtosecond UV laser irradiation with 2.37 TWcm−2 is significantly safer and more efficient toward surface contaminant desorption than sub-nanosecond laser irradiation. The results herein presented suggest that these types of fs lasers may be considered for realistic laser conservation of valuable historic and archaeological museum artifacts.
Plant Growth and the Contents of Major Bioactive Compounds of Salvia miltiorrhiza Bunge Grown in Mississippi, United States
Authors: Xing Zhiheng , Bi Guihong , Li Tongyin , Zhang Qianwen , Knight Patricia R.
Published: February 2023

Salvia miltiorrhiza Bunge (danshen) is a traditional herbal medicine and has been widely used to prevent and treat cardiovascular and other diseases. Currently, the majority of medicinal plants, including danshen, used in the United States are imported from foreign countries, which often involves challenges such as inconsistency in contents of bioactive compounds and inadequate supply of high quality plant materials to meet market demand. The objective of this study was to evaluate plant growth and the contents of major bioactive compounds of three selected danshen cultivars and identify cultivars with the greatest potential for commercial production in Mississippi. Results showed that danshen plants can grow well in Mississippi and the three cultivars evaluated had similar growth indices, leaf SPAD values, photosynthetic activities, shoot and root dry weights, and root numbers, but differed in germination rates, maximum root lengths, and maximum root diameters. The major bioactive compounds in danshen root extracts, including tanshinone I, tanshinone IIA, cryptotanshinone, and salvianolic acid B, were quantified with no significant difference in their contents among the three cultivars. Results from this study suggested that danshen has the potential to be grown as an alternative crop in Mississippi, USA.
Evaluating Optimum Limited Irrigation and Integrated Nutrient Management Strategies for Wheat Growth, Yield and Quality
Authors: Zulfiqar Usman , Ahmad Muhammad , Valipour Mohammad , Ishfaq Muhammad , Maqsood Muhammad Faisal , Iqbal Rashid , Ali Muhammad Fraz , Roy Rana , El Sabagh Ayman
Published: February 2023

Agricultural productivity is significantly influenced by the restricted availability of irrigation water and poor soil health. To assess the influence of different potential soil moisture deficit (PSMD) regimes and integrated nutrient levels on the growth, yield, and quality of wheat, an experiment was carried out at the research area of the University of Agriculture, Faisalabad. The experiment includes three levels of PSMD (I1: 25 mm PSMD, I2: 50 mm PSMD, and I3: 75 mm PSMD) and four integrated nutrition levels (N1: 50% organic manure + 50% Inorganic NPK, N2: 75% organic manure + 25% inorganic NPK, N3: 100% application of organic manure, and N4: 100% application of inorganic NPK). Results of the experiment revealed that maximum grain yield (4.78 t ha−1) was obtained as a result of irrigation at 50 mm PSMD with the combined use of organic and inorganic sources in equal proportions. In contrast, the minimum yield was observed at I3: 75 mm PSMD with 100% application of organic manure. The highest plant height (99.11 cm), fertile tillers (284.4), 1000-grain weight (44.48 g), biological yield (14.82 t ha−1), radiation use efficiency for grain yield (RUEGY) (5.71 g MJ−1), and radiation use efficiency for total dry matter (RUETDM) (2.15 g MJ−1) were observed under N1: 50% organic manure with 50% inorganic NPK treatment. The highest value of these parameters was also observed in I2 (50 mm PSMD). The results of this study can be extended to arid and semi-arid regions, where deficit irrigation is a key strategy to address water crises and to meet sustainable development goals.
Quantifying the Impact of Deployments of Autonomous Vehicles and Intelligent Roads on Road Safety in China: A Country-Level Modeling Study
Authors: Tan Hong , Zhao Fuquan , Song Haokun , Liu Zongwei
Published: February 2023

Approximately 1.35 million people lose their lives due to road traffic collisions worldwide per year. However, the variation of road safety depending on the deployment of Autonomous Vehicles (AV), Intelligent Roads (IR), and Vehicle-to-Vehicle technology (V2V) is largely unknown. In this analysis, a bottom-up analytical framework was developed to evaluate the safety benefits of avoiding road injuries and reducing crash-related economic costs from the deployment of AVs, IRs, and V2Vs in China in 26 deployment scenarios from 2020 to 2050. The results indicate that compared with only deploying AVs, increasing the availability of IRs and V2V while reducing the deployment of fully AVs can achieve larger safety benefits in China. Increasing the deployment of V2V while reducing the deployment of IRs can sometimes achieve similar safety benefits. The deployment of AVs, IRs, and V2V plays different roles in achieving safety benefits. The large-scale deployment of AVs is the foundation of reducing traffic collisions; the construction of IRs would determine the upper limit of reducing traffic collisions, and the readiness of connected vehicles would influence the pace of reducing traffic collisions, which should be designed in a coordinated manner. Only six synergetic scenarios with full equipment of V2V can meet the SDG 3.6 target for reducing casualties by 50% in 2030 compared to 2020. In general, our results highlight the importance and the potential of the deployment of AVs, IRs, and V2V to reduce road fatalities and injuries. To achieve greater and faster safety benefits, the government should prioritize to the deployment of IRs and V2V. The framework developed in this study can provide practical support for decision-makers to design strategies and policies on the deployment of AVs and IRs, which can also be applied in other countries.
Integrating Health into Local Plans: A Comparative Review of Health Requirements for Urban Development in Seven Local Planning Authorities in England
Authors: Callway Rosalie , Le Gouais Anna , Bird Emma L. , Chang Michael , Kidger Judi
Published: February 2023

A local plan is a statutory policy document that supports urban development decisions across a local government area in England. Local plans are reported to need more specific requirements for development proposals regarding wider health determinants to address potential health outcomes and health inequalities. This study reviews the integration of Health in Local Plans of seven local planning authorities through documentary analysis methods. A review framework was formulated based on health and planning literature regarding local plans, health policy and determinants of health and dialogue with a local government partner. The findings identify opportunities to strengthen the consideration of Health in Local Plans, including ensuring that policies are informed by local health priorities and signpost national guidance, strengthening health-related requirements for developers (e.g., indoor air quality, fuel poverty and security of tenure) and improving implementation of requirements for developers (e.g., through adoption of health management plans and community ownership). The study identifies further research needs regarding how policies are interpreted by developers in practice, and on national guidance for Health Impact Assessment. It highlights the benefit of undertaking a comparative review, contrasting local plan policy language and identifying opportunities to share, adapt and strengthen planning requirements regarding health outcomes.
It’s Not Just Me, It’s Us, Together: The Embodied of the Wounded Healer in the Role of Sex Trade Survival Mentors—A Critical Mentoring Perspective
Authors: Kali Dodish-Adi , Malka Menny
Published: February 2023

Mentoring is one of the unique forms of rehabilitation used to engage with women in the sex trade. The role creates personal and professional challenges; one concerns the mentors themselves dealing with a past in the sex trade, embodying within it a sign of social disgrace. Echoing the concept of the “wounded healer,” the present study examines how mentors who are sex trade survivors perceive their role in supporting the rehabilitation of women in the sex trade and the meanings that they give it. The research is based on a qualitative approach from a critical-feminist point of view. Eight female mentors and sex trade survivors, working in different settings, participated in the study. Data collection was conducted through semi-structured, in-depth interviews. Based on content analysis, the study points to four components of mentoring vis-à-vis the rehabilitation of women in the sex trade: (1) mutual identification and shared destiny; (2) corrective experience; (3) hope; and (4) saving lives. In addition, mentoring serves as a bridge for the mentors, eliciting opportunities for growth transforming out of their pain. The research findings are discussed in the context of the theoretical framework of critical mentoring, and how a relationship and a therapeutic alliance can turn mentoring into a critical healing practice, in relation to four principles: (1) equality; (2) critical empathy; (3) recognition; and (4) solidarity. The paper encourages the use of mentoring-based interventions in the process of rehabilitating women in the sex trade.
Uncertainty Quantification for Epidemic Risk Management: Case of SARS-CoV-2 in Morocco
Authors: Hammadi Lamia , Raillani Hajar , Ndiaye Babacar Mbaye , Aggoug Badria , El Ballouti Abdessamad , Jidane Said , Belyamani Lahcen , Souza de Cursi Eduardo
Published: February 2023

In this paper, we propose a new method for epidemic risk modelling and prediction, based on uncertainty quantification (UQ) approaches. In UQ, we consider the state variables as members of a convenient separable Hilbert space, and we look for their representation in finite dimensional subspaces generated by truncations of a suitable Hilbert basis. The coefficients of the finite expansion can be determined by approaches established in the literature, adapted to the determination of the probability distribution of epidemic risk variables. Here, we consider two approaches: collocation (COL) and moment matching (MM). Both are applied to the case of SARS-CoV-2 in Morocco, as an epidemic risk example. For all the epidemic risk indicators computed in this study (number of detections, number of deaths, number of new cases, predictions and human impact probabilities), the proposed models were able to estimate the values of the state variables with precision, i.e., with very low root mean square errors (RMSE) between predicted values and observed ones. Finally, the proposed approaches are used to generate a decision-making tool for future epidemic risk management, or, more generally, a quantitative disaster management approach in the humanitarian supply chain.
The Influence of Visual Input on Electromyographic Activity and Patterns of Masticatory and Cervical Spine Muscles in Emmetropic Caucasian Subjects by Gender
Authors: Zieliński Grzegorz , Matysik-Woźniak Anna , Rapa Maria , Baszczowski Michał , Pająk Beata , Ginszt Michał , Szkutnik Jacek , Rejdak Robert , Gawda Piotr
Published: February 2023

(1) Background: The objective of the research was to analyze the change of visual input on electromyographic activity and patterns of masticatory and cervical spine muscles in emmetropic Caucasian subjects by gender. Supposedly, visual input should not influence activity and electromyographic patterns of masticatory and cervical spine muscles in emmetropic Caucasian subjects by gender. (2) Methods: After applying the inclusion criteria, 50 emmetropic Caucasian subjects were included in the study. Four muscle pairs were analyzed: the temporalis muscle (TA), the masseter muscle (MM), the digastric muscle (DA), and the sternocleidomastoid muscle (SCM), during resting and functional activity. (3) Results: It was observed that there were no significant statistical differences in activity and bioelectrical patterns between open and closed eyes in women and men, with the exception of clenching on dental cotton rollers in DA-left and DA mean between tests in women. The observed statistical results had a small effect size, successively equal to 0.32 and 0.29. (4) Conclusions: Changes in the influence of visual input do not affect electromyographic activity and patterns of masticatory and cervical spine muscles in emmetropic Caucasian women and men.
Infidelity in the Adolescence Stage: The Roles of Negative Affect, Hostility, and Psychological Well-Being
Authors: Beltrán-Morillas Ana M. , Villanueva-Moya Laura , Sánchez-Hernández M. Dolores , Alonso-Ferres María , Garrido-Macías Marta , Expósito Francisca
Published: February 2023

Background: Infidelity is a relational process common in all types of romantic relationships and has been established as one of the main causes of relationship breakdown. However, little is known about this type of transgression in adolescent romantic relationships, although it manifests as a fairly frequent behavior involving different motivations. Even less is known about the emotional impact of infidelity on the offending person and its association with hostile behavior and psychological well-being. Methods: Through an experimental study (N = 301 Spanish adolescents (190 female and 111 male; Mage = 15.59, SD = 0.69; range from 15 to 17), we sought to analyze the effect of manipulating two types of motivations for infidelity (sexual vs. emotional dissatisfaction) on negative affect, hostility, and psychological well-being. Results: The main results revealed that committing infidelity motivated by hypothetical sexual (vs. emotional) dissatisfaction was indirectly related to lower psychological well-being through its effects on increased negative affect and hostility. Conclusions: Last but not least, we discuss these findings, highlighting the possible implications of infidelity for the psychosocial and psychosexual development of adolescents.
Effect of a Virtual Reality Exercise on Patients Undergoing Haemodialysis: A Randomised Controlled Clinical Trial Research Protocol
Authors: Meléndez-Oliva Erika , Sánchez-Romero Eleuterio A. , Segura-Ortí Eva , Gil-Gómez José-Antonio , Soto-Goñi Xabier A. , Poveda-Pagán Emilio J.
Published: February 2023

High levels of inflammatory markers have been associated with a greater deterioration of renal function and cardiovascular morbidity and mortality. For its part, physical exercise has been shown to be beneficial in improving the functional, psychological, and inflammatory states of patients with chronic kidney failure (CKF) undergoing haemodialysis (HD) treatment, improving their health-related quality of life. In recent years, virtual reality (VR) has been studied and described as an effective and safe tool that improves patients’ adherence to exercise programs. For these reasons, we propose to analyse the effect of VR exercise on the functional, psychological, and inflammatory states of patients on HD, as well as their levels of adherence to exercise, and compare them with static pedalling exercises. We will randomise 80 patients with CKF into two blind groups: an experimental group, which will carry out an intradialytic exercise program with non-immersive VR (n = 40), and a control group, which will exercise with a static pedal (n = 40). Functional capacity, inflammatory and phycological status, and exercise adherence will be analysed. Higher levels of adherence to exercise are expected in the VR group, which will have greater effects on the patients’ functional capacity and psychological and inflammatory status.
The Destructive Effects of Recreational Off-Highway Vehicles on Farmer Well-Being: Mental Health Consequences Outweigh Economic Losses
Authors: Gish Moshe , Shalmon Aya , Ayalon Ofira
Published: February 2023

In many countries, the use of recreational off-highway vehicles (ROVs) occasionally spills over into agricultural lands. The conflict between ROV users and farmers is escalating due to the growing popularity of ROVs. Determined action of authorities for mitigating the phenomenon may necessitate an understanding of the nature and extent of the actual damage caused by ROVs. However, it is currently unknown how ROVs harm agriculture and what is their main deleterious effect on farmers. We tested our hypothesis that economic costs are the leading reason for farmer distress using in-depth interviews with 46 Israeli farmers that are affected by ROVs. We found that contrary to our hypothesis, economic costs were low and negligible, despite high levels of anger, distress, or hopelessness expressed by almost all farmers. The main reason for outrage and frustration was the emotional impact of ROV activity on the farmers. Therefore, measuring the effects of ROVs on agriculture in terms of economic losses will probably be ineffective in convincing policymakers to act against the reckless use of ROVs in agricultural lands. On the other hand, conveying the emotional implications for the farmers can potentially promote change if accompanied by explanations on the importance of caring for the mental health and well-being of a sector that already suffers from levels of stress and mental health problems that are among the highest of any other industry in the world.
The Lifetime History of the First Italian Public Extra-Corporeal Shock Wave Lithotripsy (ESWL) Lithotripter as a Mirror of the Evolution of Endourology over the Last Decade
Authors: Malinaric Rafaela , Mantica Guglielmo , Martini Mariano , Balzarini Federica , Mariano Federico , Marchi Giovanni , Tognoni Piero , Panarello Daniele , Bottino Paolo , Terrone Carlo
Published: February 2023

Extracorporeal shockwave lithotripsy (ESWL) is the only non-invasive treatment for kidney stones. It does not require an operating room, anesthesia, or hospital stay. Its role evolved over the years and nowadays ESWL is slowly disappearing from many stone centers and urologic departments. We present the history and the role of ESWL treatment since its birth in 1959 and its development through the following years. We also present details of its application and impact on the first Italian stone center in 1985. ESWL has had different roles over the centuries: in the early years it was a great alternative to open surgery and percutaneous nephrolithotripsy (PCNL), then it had its decline with the introduction of the miniscopes. Currently, although ESWL is not considered a treatment of excellence, newer models are emerging. With the application of new technologies and artificial intelligence, this technique can become a good option alongside endourologic treatments.
Effects of a Multi-Professional Intervention on Mental Health of Middle-Aged Overweight Survivors of COVID-19: A Clinical Trial
Authors: Ryal Joed Jacinto , Perli Victor Augusto Santos , Marques Déborah Cristina de Souza , Sordi Ana Flávia , Marques Marilene Ghiraldi de Souza , Camilo Maria Luiza , Milani Rute Grossi , Mota Jorge , Valdés-Badilla Pablo , Magnani Branco Braulio Henrique
Published: February 2023

The present study aimed to investigate the effects of a multi-professional intervention model on the mental health of middle-aged, overweight survivors of COVID-19. A clinical trial study with parallel groups and repeated measures was conducted. For eight weeks, multi-professional interventions were conducted (psychoeducation, nutritional intervention, and physical exercises). One hundred and thirty-five overweight or obese patients aged 46.46 ± 12.77 years were distributed into four experimental groups: mild, moderate, severe COVID, and control group. The instruments were used: mental health continuum-MHC, revised impact scale–IES-r, generalized anxiety disorder-GAD-7, and Patient health questionnaire PHQ-9, before and after eight weeks. The main results indicated only a time effect, with a significant increase in global MHC scores, emotional well-being, social well-being, and psychological well-being, as well as detected a significant reduction in global IES-R scores, intrusion, avoidance, and hyperarousal, in addition to a reduction in GAD-7 and PHQ-9 scores (p < 0.05). In conclusion, it was possible to identify those psychoeducational interventions that effectively reduced anxiety, depression, and post-traumatic stress symptoms in post-COVID-19 patients, regardless of symptomatology, in addition to the control group. However, moderate and severe post-COVID-19 patients need to be monitored continuously since the results of these groups did not follow the response pattern of the mild and control groups.