bluuebunny/crossref_metadata_2025 huggingface dataset:
Expression Pattern of the Plasminogen Activator—Plasmin System in Human Cholesteatoma
Authors: Schönermark Matthias P. , Issing Peter R. , Erbrich Birte K. , Lenarz Thomas
Published: October 2014

The plasminogen activator—plasmin system plays a pivotal role in the delicately regulated process of extracellular matrix remodeling. Recent studies have shown that an imbalance of proteolytic enzymes over specific inhibitors in this system may lead to an aggressive, expanding, and infiltrating cellular phenotype. As cholesteatoma resembles a tumor in many ways, we investigated the pattern of expression for members of the plasminogen activator—plasmin system in 12 human cholesteatomas, using immunohistochemistry. As controls, 3 tympanic membranes and 4 ear canal skin specimens were used. In contrast to the tympanic membranes, all cholesteatoma specimens showed a strong expression of plasminogen at the basal epithelial cell layers. In ear canal skin, only the basal surface of the most basal epithelia stained discretely positive. The urokinase-type plasminogen activator (uPA) could be detected in the basal stratum of the cholesteatoma matrix and in the surrounding granulation tissue, while tissue-type plasminogen activator (tPA) was not detectable at all. Plasminogen activator inhibitor–1 (PAI-1) was expressed in both the granulation tissue and the granular cell layer of the matrix, but not in the basal epithelial cells; PAI-2 showed a pericellular expression pattern in the sub-basal and granular cell layers. Neither uPA, tPA, nor the PAIs could be detected in tympanic membrane controls; ear canal skin showed the same staining pattern as cholesteatoma only for PAI-2. Our data demonstrate that there is a clear imbalance in favor of proteolytic activity in the basal epithelial layers of the cholesteatoma matrix, which might at least partly account for the aggressive behavior of this tumorlike lesion. Further, the pattern of expression resembles the pattern described for several epithelial malignancies.
Measuring the Effects of Meniere's Disease: Results of the Patient-Oriented Severity Index (MD POSI) Version 1
Authors: Murphy Michael P. , Gates George A.
Published: October 2014

We describe the development and initial psychometric testing of a new self-report instrument for quantifying the impact of Meniere's disease (MD) on patients' lives: the Meniere's Disease Patient-Oriented Severity Index (MD POSI). Eighty-five volunteers with chronic MD took a self-administered MD POSI. The internal consistency form of reliability was determined by calculating Cronbach's α. The relationship between total MD POSI problem severity scores and responses to a global question concerning the degree to which his or her MD had changed the patient's life assessed construct-related validity. The total problem severity score yielded excellent reliability (Cronbach's α = .928). No significant age or gender effects were seen. A highly statistically significant association was noted between total problem severity scores and the degree to which the patient's life had been changed by his or her MD (Spearman rank correlation = .526, p < .0001), indicating excellent construct-related validity. The material included in the initial questionnaire provides important information that can be used clinically to quantify the impact of MD on patients' health status. A beta version of the instrument is in the process of validation and determination of its sensitivity to change in health status.
Effects of Epinephrine on Ouabain-Sensitive, K+-Dependent P-Nitrophenylphosphatase Activity in Strial Marginal Cells of Guinea Pigs
Authors: Kanoh Naoyuki
Published: October 2014

In strial marginal cells, Na+/K+-ATPase activity is abundant, and contributes to maintain the characteristic electrolyte composition of the cochlear endolymph. In the present study, to clarify the relationship between epinephrine and strial Na+/K+-ATPase activity, the ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase) activity of strial marginal cells was investigated with a cerium-based method in normal guinea pigs and guinea pigs treated with reserpine, epinephrine, and reserpine plus epinephrine. In our previous study, K+-NPPase activity had almost completely decreased 3 to 20 days after reserpine administration. In the present study, at 10 days after reserpinization and following repeated epinephrine treatment, enzyme activity was detectable. These results suggest that exogenous epinephrine was able to restore strial K+-NPPase activity in the reserpine-treated animals, and that epinephrine might increase strial Na+/K+-ATPase activity.
Anatomic Study for Posterior Medialization Thyroplasty
Authors: Kojima Hisayoshi , Hirano Shigeru , Shoji Kazuhiko , Isshiki Nobuhiko
Published: October 2014

To attain posterior medialization by thyroplasty type I, we simulated the surgery using 3 cadaveric larynges. Two approaches were applied to adduct the arytenoid cartilage: one involved compression of the vocal process, and the other, compression of the muscular process inward. The inner perichondrium was incised to reach the arytenoid cartilage from the framework. To compress the vocal process, a large silicone plug was required. In this method, there was some difficulty in compressing the vocal process precisely, and there was the risk of postoperative extrusion of the large plug. In contrast, the muscular process was more superficial relative to the framework, and it could be precisely compressed, resulting in sufficient posterior glottal closure. The silicone plug required for this procedure has a shallow depth that may provide the advantage of preventing postoperative extrusion. In conclusion, the compression of the muscular process is preferable for safely achieving posterior glottal closure.
Clinical Presentation and Management of Labyrinthine Fistula Caused by Chronic Otitis Media
Authors: Busaba Nicolas Y.
Published: October 2014

To describe the clinical presentation and surgical management of patients with chronic otitis media complicated by labyrinthine fistula and to determine clinical indicators that predict postoperative hearing outcome, I performed a retrospective analysis at an academic tertiary care center. Thirty-four patients with labyrinthine fistula as a complication of chronic otitis media, documented at mastoidectomy, underwent postoperative audiometry. The median age was 50 years, and the duration of otologic symptoms ranged from 2 months to more than 40 years. On presentation, 3 patients had anacusis in the affected ear, while in the others, the pure tone average for bone conduction at the 0.5-, 1-, 2-, and 4-kHz frequencies was 34 dB hearing level. Nineteen patients (56%) complained of dizziness on presentation. The fistula test was positive in 14 of 28 patients (50%). The fistula was detected radiologically in 10 of 24 patients (42%). Cholesteatoma was present in 33 of 34 patients (97%). The lateral semicircular canal was the most common site of labyrinthine fistula. The cholesteatoma matrix was completely removed in 29 of 33 cases and exteriorized in the remaining 4. Of the 31 patients with measurable hearing preoperatively, anacusis occurred in 8 (26%). In 6 of these, the preoperative pure tone average for bone conduction was greater than 50 dB hearing level, and cholesteatoma matrix and granulation tissue invading the membranous labyrinth were found at surgery. I concluded that in chronic otitis media, labyrinthine fistulas occurred almost exclusively in the presence of a cholesteatoma. Postoperative hearing outcome correlated with the size of the fistula and the presence of granulation tissue invading the labyrinth, which could be predicted by the preoperative audiometry.
Effect of Platelet Activating Factor and its Antagonist on the Mucociliary Clearance of the Eustachian Tube in Guinea Pigs
Authors: Rhee Chung-Ku , Jeong Pil-Seob , Chung Phil-Sang , Kim Young-Hoon , Jung Timothy T. K.
Published: October 2014

The purpose of this study was to test whether platelet activating factor (PAF) impairs the mucociliary clearance function of the eustachian tube (ET) in a dose-dependent manner and whether PAF antagonist can prevent the impairment of mucociliary function of the ET induced by PAF. Coomassie brilliant blue dye transport time (DTT) in normal guinea pigs was 69 seconds. The DTTs after the application of normal saline and PAF at 1 and 2 μg/mL into bullae were 66, 74, and 157 seconds. The time was over 15 minutes when 4, 8, and 16 μg/mL of PAF were applied. The DTT was 62 seconds when the animals were pretreated with PAF antagonist (WEB 2170). There were significant delays of the DTTs after treatment with 2, 4, 8, and 16 μg/mL of PAF. Histopathologic examination of ETs from groups with a significant delay in DTTs showed intact cilia, mucous plugs, increased inflammatory cells, and exfoliation of cells. This study demonstrated that PAF impaired the mucociliary clearance function of the ET in a dose-dependent manner. This impairment of mucociliary clearance function was prevented by pretreatment with PAF antagonist. The findings of the study suggest that PAF plays an important role in the pathogenesis of otitis media with effusion by impairing the ET clearance function.
Prospective Application of a Protocol for Selective Nonsurgical Management of Suspected Orbital Subperiosteal Abscesses in Children
Authors: Sajjadian Ali , Chundru Usha , Isaacson Glenn
Published: October 2014

Goodwin et al (1982) and Souliere et al (1990) have proposed a protocol for the selective nonsurgical management of suspected orbital subperiosteal abscesses. This protocol has been routinely applied by the Temple Pediatric Otolaryngology and Ophthalmology services since 1990. During this period, children with computed tomography and physical examination findings consistent with orbital subperiosteal abscesses and visual acuity better than 20/60 were treated with intravenous antibiotics rather than immediate surgery. They were followed with frequent ophthalmologic examinations and operated upon if vision deteriorated or they failed to improve clinically within 48 hours. A review of patients admitted to St Christopher's Hospital for Children, the site of Temple University's pediatric activities in the years from 1991 to 1997, revealed 14 patients with clinical evidence of orbital subperiosteal abscesses. Twelve patients were treated in accordance with the Goodwin-Souliere protocol. All recovered completely without surgical intervention and without any adverse sequelae. Selective nonsurgical treatment of clinically diagnosed orbital subperiosteal abscesses in children is relatively safe and effective.
Substernal Goiter: Clinical Experience of 72 Cases
Authors: Hernandez Quiteria , Soria Teresa , Rodriguez José M. , Piñtero Antonio , Ramirez Pablo , Ortiz Sergio , Parrilla Pascual
Published: October 2014

The aim of this work was to study the clinical management and surgical approach of substernal goiters. We studied the clinical data, preoperative evaluation, surgical treatment, histopathologic data, complications, and follow-up of 72 patients found to have substernal goiter over a period of 15 years, from a total of 780 patients with goiter who underwent surgery. In this group, 83% were women, and the mean age was 61 years. The most common symptoms were the existence of a palpable cervical mass (93% of cases) and dyspnea (40%). The most successful study to diagnose substernal goiter was computed tomography (100%), followed by chest radiography (75%), gammagraphy (19%), and ultrasound (15%). All but 7 patients received a Kocher cervicotomy, and 49% of the cases underwent a total thyroidectomy. The histologic study revealed 3 carcinomas (4%). There was 1 permanent unilateral recurrent laryngeal nerve injury (1.4%) and 1 instance of permanent hypoparathyroidism (1.4%). We regard surgery as the most successful treatment for patients with substernal goiter, even in those without compressive symptoms. We base our choice on the low morbidity and zero mortality obtained.
Postnatal Development of Myosin Heavy Chain Isoforms in Rat Laryngeal Muscles
Authors: Shiotani Akihiro , Jones Richard M. , Flint Paul W.
Published: October 2014

The developmental transitions of myosin heavy chain (MHC) isoforms of rat posterior cricoarytenoid (PCA), thyroarytenoid (TA), cricothyroid (CT), and lateral cricoarytenoid (LCA) muscles were examined by means of sodium dodecyl sulfate—polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot techniques. The muscles were microscopically dissected from animals on postnatal days 0, 3, 7, 10, 14, 21, 28, 35, 45, and 55 and from adult animals. Silver-stained SDS-PAGE gels of each muscle were analyzed densitometrically to measure the composition of MHC isoforms, and Western blot was carried out to identify specific bands. Characterizations of the internal laryngeal muscles determined by the composition of MHCs were correlated with their function in the adult. Temporally, differentiation reflects onset of function. Differentiation of isoforms and transition to adult forms occur first in the TA muscle, followed by the PCA, LCA, and CT muscles. Expression of type IIL was observed only in muscles innervated by the recurrent laryngeal nerve. Postnatally observed developmental differences of myosin phenotypes suggest that regulation of MHC expression is influenced by neural activity or other environmental factors.
Graft Healing in Laryngotracheal Reconstruction: An Experimental Rabbit Model
Authors: Jacobs Ian N. , Boden Scott D. , Podrebarac Pierre , Chen Michael
Published: October 2014

The actual sequence of events in graft healing in laryngotracheal reconstruction (LTR) is not well understood. To investigate sequential changes in graft healing, we submitted 21 rabbits to LTR with an anterior auricular cartilage graft. They were immediately extubated after surgery, and no stent was used. Rabbits were painlessly sacrificed at 7 different time periods (0, 1, 2, 3, 4, 6, and 10 weeks after surgery). Cross sections of the larynx and graft were cut and stained with hematoxylin-eosin and trichrome. Epithelialization progressed very rapidly and was complete by 21 days. During the 10 weeks, there was a progressive necrosis and resorption of the original graft cartilage. However, neochondrification progressed very rapidly and resulted in excellent structural support of the graft in the airway. Problems, such as infection and granulation tissue formation, were relatively minor and infrequent.
Swallowing Disorders in Paralysis of the Lower Cranial Nerves: A Functional Analysis
Authors: Périé Sophie , Hazebroucq Vincent , Coiffier Laurent , Chaussade Stanislas , Laccourreye Laurent , St Guily Jean Lacau
Published: October 2014

Deficits of the lower cranial nerves (nerves IX, X, XI, and XII) occurring after treatment of skull base tumors may cause disabling swallowing disorders. To assess the mechanisms of swallowing disorders involved in such cases, we performed functional examinations: a videoendoscopic swallowing study and simultaneous manometry and videofluoroscopy in 7 patients. This study shows that the main mechanism of the swallowing disorders was a disturbance of the pharyngeal stage, including a decrease of pharyngeal propulsion, reduced laryngeal closure, and cricopharyngeal dysfunction, which led to aspiration. Decreased pharyngeal propulsion was found in 6 patients, with a very high correlation between fiberoscopy and simultaneous manometry-fluoroscopy. The responsibility of the upper esophageal sphincter in swallowing disorders was more difficult to assess. The role of the upper esophageal sphincter and pharyngeal propulsion in the onset of the problem is discussed in regard to the cricopharyngeal myotomy.
Do Cochlear Nuclei Contribute to Auditory Lateralization?
Authors: Aǧar Erdal , Korkmaz Adnan , Bosnak Mehmet , Demir Serif , Ayyildiz Mustafa , Marangoz Cafer
Published: October 2014

The aim of this study was to estimate the total number of cells in the subdivisions of the cochlear nucleus (CN) of the rat with unbiased stereological methods. The total number of neurons was determined in both the ventral cochlear nucleus (VCN) and the dorsal cochlear nucleus (DCN) to compare the right and left sides. The total cell numbers were 15,280 in the left VCN, 15,400 in the right VCN, 10,260 in the left DCN, and 10,860 in the right DCN. Comparison of the right and left major subdivisions of the CN of the rat showed that there was no significant difference between the right and left sides of the rat CN. This result indicates that the CNs do not contribute to auditory lateralization in the rat in regard to cell numbers.
Severe Pharyngeal Stenosis Treated with Inferiorly Based Sternocleidomastoid Myocutaneous Flap
Authors: Haller Jeffrey R. , Gray Steven D.
Published: October 2014

Severe pharyngeal stenosis is a debilitating condition associated with apnea and dysphagia. Treatment options include local flaps and free mucosal grafts. We present 2 cases of severe stenosis, apparently from adenotonsillectomy, that failed more conservative repairs. Both were treated successfully with a sternocleidomastoid myocutaneous flap rotated in through a lateral pharyngotomy. No further treatment has been required. Technical considerations and operative planning are discussed.
Prevention of Airway Complications in Thyroplasty Patients Requiring Endotracheal Intubation
Authors: Friedlander Paul , Aygene Erdine , Kraus Dennis H.
Published: October 2014

Patients who have undergone silicone vocal cord medialization and require additional surgery are at risk for airway complications. There is a narrowed glottic aperture that may be prone to develop postoperative laryngeal edema and prosthesis extrusion. This study was designed to assess the management of this difficult airway and to determine the frequency of postintubation complications. We identified 82 patients who had undergone vocal cord medialization with silicone implants between 1991 and 1995. Seventeen of these patients underwent additional surgical procedures requiring general anesthesia. A retrospective review of these patients' charts was performed to determine the management of the airway and the incidence of postintubation complications. There were no postintubation complications in the 17 patients who were studied. The duration of surgery ranged from 40 minutes to 4 hours 15 minutes. Two patients were ventilated via bronchoscope, and 15 patients were intubated orally. The endotracheal tubes ranged from size 6 to size 9 (median size 8). None of the patients required perioperative steroids. All patients were successfully extubated in the recovery room. No patients required intubation or tracheotomy, and there were no implant extrusions. In this study, the incidence of postintubation airway complications in patients who had undergone previous thyroplasty was minimal. Nevertheless, the potential for airway compromise exists. We recommend preoperative discussion with the anesthesiologist, atraumatic intubation with a small endotracheal tube, and diligent observation for airway compromise.
Inflammatory Response to Chronic Otitis Media in Digeorge Syndrome: A Case Study Using Immunohistochemistry on Archival Temporal Bone Sections
Authors: Ganbo Tetsuya , Sando Isamu , Suzuki Chiaki , Balaban Carey D. , Kitagawa Miwa
Published: October 2014

Immunohistochemical analyses were conducted on archival celloidin-embedded human temporal bone sections from an 8-month-old boy with chronic otitis media and DiGeorge syndrome. We employed antigen retrieval methods with saturated sodium hydroxide—methanol solution, microwave incubation, and proteolytic treatment to demonstrate the distribution of T-lymphocytes, B-lymphocytes, macrophages, and intercellular adhesion molecule 1 (ICAM-1) expression in the middle ear. B-lymphocytes and macrophages were observed predominantly within the middle ear mucosa. T-lymphocytes were rare. Further, ICAM-1 was expressed in the vascular endothelium of the lamina propria, as well as infiltrating mononuclear cells. This suggests that the expression of ICAM-1 can be induced in the middle ear with otitis media, even if T-lymphocytes are depressed in a cell-mediated immunodeficiency disorder such as DiGeorge syndrome.
Mucin Gene Expression in the Rat Middle Ear: An Improved Method for Rna Harvest
Authors: Lin Jizhen , Ho Samuel , Paparella Michael M. , Shekels Laurie , Kim Youngki
Published: October 2014

Mucins are heavily glycosylated proteins characterized by high molecular weight and heterogeneous structure. Mucin genes are expressed in a tissue- or epithelium-specific manner. Although mucins are known to be important structural components of the mucociliary transport system that protects epithelium against invading microorganisms, very little is known about mucin gene expression unique to the middle ear. This study demonstrated that middle ear messenger RNA specifically hybridized with rat MUC2 and human MUC2 (SMUC-41) complementary DNA probes. MUC3 and MUC5AC mucin genes, dominantly expressed in rodent intestine and trachea, were not detected in the rat middle ears in this study. The middle ear MUC2 messenger RNA harvested by lavage was characterized by a single transcript — unlike its counterpart in intestine and airways, which is characterized by polydispersity — suggestive of a better method for RNA analysis. It was concluded that rat middle ears possess a MUC2 mucin gene or homologue of human MUC2 (SMUC-41).
Correcting Vocal Fold Immobility by Autologous Collagen Injection for Voice Rehabilitation
Authors: Remacle Marc , Delos Monique , Lawson Georges , Jamart Jacques
Published: October 2014

We report on a short-term clinical study of injectable autologous collagen (Vocalogen) used to correct dysphonias arising from vocal fold immobility. The collagen is extracted from skin taken from the lower abdominal quadrant area or from just above the bikini line. About 30 cm2 of skin are required to provide 2 mL of injectable collagen. The histologic examination of the preparation before injection disclosed the presence of elastin fibers and some clusters of epithelial cells, beside the collagen fibers. The collagen is naturally reticulated, and the molecule is preserved in its entirety. The technique is exactly the same as that reported for bovine collagen: injection into Reinke's space, under general anesthesia, monitored by direct microlaryngoscopy. The amount injected is also similar: 1.5 mL for correction of glottic insufficiency in which the immobile vocal fold is in the intermediate position. Eight patients were injected, and the average follow-up was 4.5 months. Voice assessments made before and after the treatment included stroboscopy, subjective and perceptual judgments, and aerodynamic and acoustic measurements. The functional results were similar to those obtained with bovine collagen. No complications arose. The probability of any hypersensitivity reaction, always a possibility to be feared with bovine collagen, is negligible with the autologous collagen. Long-term results are as yet unknown, but from the fact that the collagen molecular structure is intact and there is little risk of foreign body response, it would be expected that autologous preparations would be more stable than bovine collagen; this appears to be the case in cosmetic applications. Autologous collagen could be employed for the same indications as bovine collagen, provided that a delay of 45 days (the time required to prepare the injectable collagen) is acceptable. The amount of collagen required is also a limiting factor, since the patient's own skin is the starting donor material.
Transoral Carbon Dioxide Laser Resection of Supraglottic Carcinoma
Authors: Rudert Heinrich H. , Werner Jochen A. , Höft Steffen
Published: October 2014

Between 1981 and 1994, 34 patients with squamous cell carcinoma of the supraglottis were treated by transoral carbon dioxide laser resection, 12 of them palliatively. Additional treatment included neck dissection in 21 patients and radiotherapy in 24 patients. The 3-year overall survival was 62%, and the actuarial survival 80%. The overall survival for T1 and T2 tumors was 71%, and that for T3 and T4 tumors was 47%. The overall 3-year survival for the early stages, I and II, was 88%, and that for the advanced stages, III and IV, was 50%. These results are comparable to the outcome after conventional open partial resection. Given the significantly lower morbidity (only 7 patients required tracheostomy), we do not observe an age limit anymore. The transoral method can be recommended as curative treatment in T1 and T2 tumors and in selected T3 and T4 tumors in concert with neck dissection and/or radiotherapy. In patients with advanced inoperable tumors, laser surgery is an excellent alternative to tracheostomy and palliative radiotherapy. Prerequisites for successful application of the transoral carbon dioxide laser resection are adequate resection techniques.
Unilateral Tonsillar Enlargement and Tonsillar Lymphoma in Children
Authors: Berkowitz Robert G. , Mahadevan Murali
Published: October 2014

The clinical presentation and surgical and pathological findings of 46 children with unilateral tonsillar enlargement (UTE; age range 2 to 13 years, mean age 6.5) who underwent tonsillectomy for biopsy purposes between 1975 and 1995 were compared with those of 7 children who received treatment for tonsillar lymphoma (TL; age range 2 to 9 years, mean age 4.8) during the same period. There was no history of rapid tonsillar enlargement in children in the UTE group, and only 20 (43%) were symptomatic. Symptoms included recurrent sore throats in 10 patients (22%), snoring in 5 (11%), nasal obstruction in 4 (9%), and dysphagia in 1 (2%). No children had systemic symptoms or significant cervical lymphadenopathy. In contrast, tonsillar enlargement was observed to occur within a 6-week period in all children with TL, and 6 (86%) children had symptoms at presentation that included dysphagia in 5 (71%), snoring in 3 (43%), night sweats in 2 (29%), and fever and rigors in 2 (29%). Cervical lymphadenopathy greater than 3 cm was present in 6 (86%) children, while 1 child (14%) had hepatosplenomegaly. There was no histopathologic evidence of neoplasia in the UTE group, and a true discrepancy in size between the two tonsils was confirmed in only 21 of 44 (48%) cases. All 7 patients in the TL group had non-Hodgkin's lymphoma. All received chemotherapy, with 5 of the 7 cured and 2 dying of disease. The data suggest that tonsillectomy should be performed for biopsy purposes in UTE where there is a history of progressive enlargement, significant upper aerodigestive tract symptoms, systemic symptoms, suspicious appearance of the tonsil, cervical lymphadenopathy, or hepatosplenomegaly. The diagnosis of TL should also be considered when UTE is present in an immunocompromised child or one with a previous malignancy, when acute tonsillitis is asymmetric and unresponsive to medical treatment, or when rapid bilateral tonsil enlargement occurs. Observation is appropriate management for other cases of UTE.
Coexpression of Neurotrophic Growth Factors and Their Receptors in Human Facial Motor Neurons
Authors: Li Jing Min , Brackmann Derald E. , Hitselberger William E. , Linthicum Fred H. , Lim David J.
Published: October 2014

Neuronal development and maintenance of facial motor neurons is believed to be regulated by neurotrophic growth factors. Using celloidin-embedded sections, we evaluated immunoreactivity of 11 neurotrophic factors and their receptors in facial nuclei of human brain stems (4 normal cases, and 1 from a patient with facial palsy and synkinesis). In the normal subjects, positive immunoreactivity of the growth factor neurotrophin-4 and acidic fibroblast growth factor (aFGF) was observed in facial motor neurons, as was positive immunoreactivity against ret, the receptor shared by glial cell line—derived neurotrophic factor and neurturin. Immunoreactivity was moderate for the receptor trkB and strong for trkC. In the case of partial facial palsy, surviving cells failed to show immunoreactivity against neurotrophins. However, immunoreactivity of aFGF was up-regulated in both neuronal and non-neuronal cells in this patient. Results suggest that these trophic growth factors and their receptors may protect facial neurons from secondary degeneration and promote regrowth of the facial nerve after axotomy or injury.
Immortalization of Chinchilla Middle Ear Epithelial Cells by Adenovirus 12-Simian Virus 40 Hybrid Virus
Authors: Jin Sunji , Rhim Johng S. , Gu Xin-Xing , Lim David J.
Published: October 2014

In order to study the cellular and molecular mechanisms of the pathogenesis of otitis media, a chinchilla middle ear epithelial cell line (CMEE-1) with differentiated cell characteristics was established by infection of a primary culture with the adenovirus 12-simian virus 40 (Ad12-SV40) hybrid. This cell line has been in continuous culture for 42 passages, whereas the parent cells underwent senescence and died at the 8th passage. The cell line also retains epithelial morphology and expresses cytokeratin polypeptides 4, 7, and 18, characteristic markers for epithelia. In Western blots of cell proteins, bands at 94 and 53 kd were labeled after binding antibodies against SV40 large T antigen and p53, respectively. Karyotype analysis showed that the cell line is derived from chinchilla epithelial cells. These findings confirm that the cell line is a chinchilla epithelial cell immortalized by the hybrid virus.
Electron Microscopic and Immunohistochemical Investigation of Reinke's Edema
Authors: Sato Kiminori , Hirano Minoru , Nakashima Tadashi
Published: October 2014

Examination was made of the vocal fold mucosa of Reinke's edema immunohistochemically and electron microscopically, with special attention to blood vessels in Reinke's space (RS). Ten surgical specimens of Reinke's edema were used, and the results obtained are summarized as follows. 1) There were subepithelial vascularization and many dilated vessels in RS. 2) The vessels had thin endothelium with many fenestrae, many vesicles, and a thickened basement membrane. 3) Plasma exuded from the capillaries into surrounding tissue via fenestrae. 4) These findings demonstrate increased permeability of the vessels. 5) The vascular walls were thin, with few pericytes. 6) The pericytes were situated away from the endothelial cells. 7) Some endothelial cells and pericytes had degenerated, and vessel occlusion was apparent in some cases. 8) These findings indicate the vessels had become fragile. 9) Interstitial cells and/or inflammatory cells in RS showed stained cytoplasm with vascular endothelial growth factor. 10) This growth factor may possibly enhance capillary permeability and form fragile vessels in RS. Fragility and alteration in permeability of the vessels are presumed to cause edema of RS, which may progress to Reinke's edema.
Characterization of Human Papillomavirus in Airway Papillomas by Histologic and Biochemical Analysis
Authors: Glynn Matthew , Sanford Thomas , Hoover Larry , Kinsey William , Dobbs Larry , Bruegger Daniel
Published: October 2014

The role of human papillomavirus (HPV) in airway papillomas has been well defined in recent literature. The chronicity and recurrence of papillomas has been postulated to be a result of residual viral genome in tissue treated with standard laser techniques. Thirteen patients with airway papillomas were selected for study with polymerase chain reaction (PCR) methods to detect viral DNA. Specimens taken prior to laser therapy and specimens taken at laser margins were consistently positive for HPV DNA by PCR. The HPV DNA is apparently present in tissues after macroscopic disease has been ablated by laser techniques. Histologic analysis of laser biopsies demonstrated fragments of squamous epithelium with cytologic features of HPV infection. Laser treatment is ineffective in eradicating HPV-infected tissues from airway papillomas, and this finding supports the notion that recurrence is a product of HPV incorporated into tissue not ablated by laser irradiation. Specific methods, results, and clinical correlation will be discussed.
Review of Sinus Histiocytosis with Massive Lymphadenopathy (Rosai-Dorfman Disease) of Head and Neck
Authors: Carbone Antonino , Devaney Kenneth O. , Passannante Alberto , Rinaldo Alessandra , Gloghini Annunziata , Ferlito Alfio
Published: October 2014

The entity known as sinus histiocytosis with massive lymphadenopathy (SHML), or Rosai-Dorfman disease (RD disease), is an uncommon benign proliferation of hematopoietic and fibrous tissue that often presents in the head and neck region. Its initial manifestations most often include a roughly symmetric, painless, bilateral cervical adenopathy, although extranodal disease may develop in a minority of patients. The key histologic feature of SHML is the presence of various numbers of large, pale histiocytic cells that contain within their cellular borders apparently engulfed lymphocytes (“emperipolesis”); these distinctive large, pale cells — RD cells — are S-100 protein—positive by immunostaining and so differ from ordinary histiocytes. Despite its sometimes impressive clinical presentation, SHML is a benign and self-limited disease, whose treatment is aimed largely at controlling its local manifestations (most often by surgical therapy). The microscopic differential diagnosis, particularly in extranodal disease, is at times challenging and can include Langerhans' cell histiocytosis, Hodgkin's disease, non-Hodgkin's lymphoma, metastatic carcinoma, and metastatic malignant melanoma.
What Is Reflection? Looking for Clarity in an Ambiguous Notion
Authors: Clarà Marc
Published: October 2014

The notion of reflection nowadays is considered crucial in the field of teaching and teacher education. However, although the great majority of approaches to reflection are grounded on the same main theoretical sources, the meaning of this notion is unanimously recognized in the field to be ambiguous. This article aims to look for clarity about what reflection is, what it is not, and how it works, by closely revisiting the seminal works of Dewey, Schön, and Wertheimer. It is argued that reflection is a descriptive notion—not a prescriptive one—and that it refers to the thinking process engaged in giving coherence to an initially unclear situation. The article then identifies some aspects of how reflection works, and some current widespread assumptions about reflection, which are insufficiently warranted, either theoretically in the writings of Dewey or Schön or empirically in the observations of reflection processes.
‘I don’t think we’ll ever be finished with this’: Fear and safety in policy and practice
Authors: Sandberg Linda , Rönnblom Malin
Published: October 2014

In planning contexts, safety is often discussed from a women’s perspective. An ideal site for exploring some of the key issues is Umeå, a medium-sized town in northern Sweden. Here, attention to women’s fear of violence greatly increased at the turn of the century, when a single repeat offender known as the ‘Haga Man’ assaulted several women in the city. People’s (especially women’s) fear of violence came to be seriously recognised, discussed and taken into consideration in the city’s planning. The present research is based on an analysis of empirical data collected in 2008, through interviews with people who in various ways work to increase safety in Umeå. The paper addresses how the informants define the problem of fear of violence in public space and the strategies they employ to address it, what could be described as the analytical-practice paradox, as the results show the difficulties of integrating gender-aware planning into planning practice.
Balancing Competing Motives
Authors: Sacco Donald F. , Young Steven G. , Hugenberg Kurt
Published: October 2014

The current research provides novel evidence for motivational trade-offs between the two fundamental human goals of pursuing social affiliation and avoiding disease. In Study 1, participants completed a writing prime that manipulated inclusionary status and found that socially excluded participants indicated lower feelings of current disease susceptibility compared with control and socially included participants. In Study 2, participants were included or excluded via Cyberball and then indicated their preferences for symmetrical versus asymmetrical faces. Socially excluded participants displayed lower preferences for symmetrical faces—a cue associated with greater disease resistance. Finally, in Study 3, participants were primed with either disease threat or a general negative affective state and then indicated their current affiliation interest. Activated disease concerns uniquely led participants to display less interest in social affiliation. Taken together, affiliation needs result in disease avoidance down-regulation to aid reaffiliation, whereas disease concerns result in affiliation down-regulation to facilitate pathogen avoidance.
The Face–Time Continuum
Authors: Hehman Eric , Leitner Jordan B. , Freeman Jonathan B.
Published: October 2014

Aging influences how a person is perceived on multiple dimensions (e.g., physical power). Here we examined how facial structure informs these evolving social perceptions. Recent work examining young adults’ faces has revealed the impact of the facial width-to-height ratio (fWHR) on perceived traits, such that individuals with taller, thinner faces are perceived to be less aggressive, less physically powerful, and friendlier. These perceptions are similar to those stereotypically associated with older adults. Examining whether fWHR might contribute to these changing perceptions over the life span, we found that age provides a shifting context through which fWHR differentially impacts aging-related social perceptions (Study 1). In addition, archival analyses (Study 2) established that fWHR decreases across age, and a subsequent study found that fWHR mediated the relationship between target age and multiple aging-related perceptions (Study 3). The findings provide evidence that fWHR decreases across age and influences stereotypical perceptions that change with age.
The Prowess and Pitfalls of Bayesian Structural Equation Modeling
Authors: Stromeyer William R. , Miller Jason W. , Sriramachandramurthy Rajendran , DeMartino Richard
Published: October 2014

Muthén and Asparouhov introduced an approach for conducting Bayesian inference in the context of structural equation models that they termed Bayesian structural equation modeling (BSEM). In this article, we provide an overview of the BSEM technique, illustrate how this technique relates to confirmatory and exploratory factor analysis, and highlight several key problems with using the BSEM approach as it is currently advocated. Utilizing data from a large-scale study of entrepreneurial self-efficacy, we develop a modified approach for applying the BSEM technique in a manner that is more consistent with accepted principles of reflective measurement, factor analysis, and model selection. We devise a series of recommendations to guide future use of the BSEM technique to help ensure that mainstream use of this approach heralds the coming of a new day in measurement development rather than a false dawn.
Evidence of a suffocation alarm system sensitive to clinically-effective treatments with the panicolytics clonazepam and fluoxetine
Authors: Schimitel Fagna Giacomin , Müller Cláudia Janaina Torres , Tufik Sérgio , Schenberg Luiz Carlos
Published: October 2014

Dyspnea, ‘hunger for air’, and the urge to flee are the cardinal symptoms of respiratory-type panic attacks. Patients also show baseline respiratory abnormalities and a higher rate of comorbid and antecedent respiratory diseases. Panic attacks are also precipitated by both the infusion of 0.5 M sodium lactate and the inhalation of 5–7% carbon dioxide (CO2) in predisposed patients, but not in healthy volunteers nor patients without panic disorder. Further studies show that patients with panic are also hyper-responsive to hypoxia. These and other observations led Klein (1993) to suggest that clinical panic is the misfiring of a suffocation alarm system. In rats, cytotoxic hypoxia of chemoreceptor cells by intravenous injection of potassium cyanide (KCN) produces short-lasting flight behaviors reminiscent of panic attacks. KCN-induced flight behaviors are blocked both by denervation of chemoreceptor cells and lesion of dorsal periaqueductal gray matter, a likely substrate of panic. Herein, we show that KCN-evoked flight behaviors are also attenuated by both acute and chronic treatment with clonazepam (0.01–0.3 mg/kg, intraperitoneally (i.p.)) and fluoxetine (1–4 mg/kg/day, i.p. for 21 days), respectively. Attenuation of KCN-evoked panic-like behaviors by clinically-effective treatment with panicolytics adds fresh evidence to the false suffocation alarm theory of panic disorder.
Investigation of the effects of solid lipid curcumin on cognition and mood in a healthy older population
Authors: Cox Katherine HM , Pipingas Andrew , Scholey Andrew B
Published: October 2014

Curcumin possesses many properties which may prevent or ameliorate pathological processes underlying age-related cognitive decline, dementia or mood disorders. These benefits in preclinical studies have not been established in humans. This randomized, double-blind, placebo-controlled trial examined the acute (1 and 3 h after a single dose), chronic (4 weeks) and acute-on-chronic (1 and 3 h after single dose following chronic treatment) effects of solid lipid curcumin formulation (400 mg as Longvida®) on cognitive function, mood and blood biomarkers in 60 healthy adults aged 60–85. One hour after administration curcumin significantly improved performance on sustained attention and working memory tasks, compared with placebo. Working memory and mood (general fatigue and change in state calmness, contentedness and fatigue induced by psychological stress) were significantly better following chronic treatment. A significant acute-on-chronic treatment effect on alertness and contentedness was also observed. Curcumin was associated with significantly reduced total and LDL cholesterol and had no effect on hematological safety measures. To our knowledge this is the first study to examine the effects of curcumin on cognition and mood in a healthy older population or to examine any acute behavioral effects in humans. Results highlight the need for further investigation of the potential psychological and cognitive benefits of curcumin in an older population.
Identifying evidence for past mining and metallurgy from a record of metal contamination preserved in an ombrotrophic mire near Leadhills, SW Scotland, UK
Authors: Mighall Tim , Martínez Cortizas Antonio , Silva Sánchez Noemí , Foster Ian DL , Singh Surjit , Bateman Mark , Pickin John
Published: October 2014

This study presents a new 3600-year record of past metal contamination from a bog located close to the Leadhills and Wanlockhead orefield of southwest Scotland. A peat core, collected from Toddle Moss, was radiocarbon (14C) dated and analysed for trace metal concentrations (by EMMA) and lead isotopes (by ICP-MS) to reconstruct the atmospheric deposition history of trace metal contamination, in particular, lead. The results show good agreement with documented historical and archaeological records of mining and metallurgy in the region: the peak in metal mining during the 18th century, the decline of lead mining during the Anglo-Scottish war and lead smelting during the early medieval period. There may also have been earlier workings during the Late Bronze and Iron Ages indicated by slight increases in lead concentrations, the Pb/Ti ratio and a shift in 206Pb/207Pb ratios, which compare favourably to the signatures of a galena ore from Leadhills and Wanlockhead. In contrast to other records across Europe, no sizeable lead enrichment was recorded during the Roman Iron Age, suggesting that the orefield was not a significant part of the Roman lead extraction industry in Britain. These findings add to the various strands of archaeological evidence that hint at an early lead extraction and metallurgical industry based in southern Scotland. The results also provide further evidence for specific regional variations in the evolution of mining and metallurgy and an associated contamination signal during prehistoric and Roman times across Europe.
The ‘Little Ice Age’ in the Southern Hemisphere in the context of the last 3000 years: Peat-based proxy-climate data from Tierra del Fuego
Authors: Chambers Frank M , Brain Sally A , Mauquoy Dmitri , McCarroll Julia , Daley Tim
Published: October 2014

The so-called ‘Little Ice Age’ (LIA) of the 15th to 19th centuries ad is well-attested from much of Europe and from some other parts of the Northern Hemisphere. It has been attributed to solar forcing, associated with reduced solar activity, notably during the Spörer, Maunder and Dalton solar minima, although other causes have also been proposed and feature strongly in recent papers. Detection of the LIA in some proxy-climate records from the Southern Hemisphere is less clear, leading to suggestions that the LIA was perhaps not a global phenomenon. Resolving this issue requires more data from the Southern Hemisphere. We present proxy-climate data (plant macrofossils; peat humification) covering the past three millennia from an ombrotrophic mire (peat bog) in Tierra del Fuego, southern South America, but focus our discussion on the period traditionally associated with the LIA. During parts of this time, the mire surface was apparently relatively dry compared with much of its 3000-year record. It was reported earlier that a particularly dry episode in the mire coincided with the 2800 cal. BP ‘solar’ event (since identified as a Grand Solar Minimum), which was attributed to solar-driven changes in atmospheric circulation, and more specifically to a shift in position of the Westerlies. Parts of the LIA record show a similar shift to dryness, and we invoke a similar cause. The shifts to and from dry episodes are abrupt. These new data support the concept of a global LIA, and for at least the intense dry episodes might reinforce the claim for solar forcing of parts of the LIA climate.
Workplace representation, its impact on trade union members and its capacity to compete with management in the European workplace
Authors: Waddington Jeremy
Published: October 2014

Drawing on questionnaire-based survey data comprising responses from the members of 14 trade union organizations based in 12 European countries, this article explores the impact of workplace representatives on perceptions of the performance of trade unions among members. The article shows that large numbers of members are dissatisfied with the quality of representation, even if a workplace representative is located at their place of work. When there is a workplace representative present, it is apparent that members view the performance of the union to be superior to that of management on a wide range of workplace tasks. When there is no workplace representative present, the performance of management is generally superior to that of the union.