bluuebunny/crossref_metadata_2025 huggingface dataset:
Preoperative Decolonization Appears to Reduce the Risk of Infection in High-Risk Groups Undergoing Total Hip Arthroplasty
Authors: Scharf Markus , Holzapfel Dominik Emanuel , Ehrnsperger Marianne , Grifka Joachim
Published: May 2023

Background: Periprosthetic infections represent a major challenge for doctors and patients. The aim of this study was therefore to determine whether the risk of infection can be positively influenced by preoperative decolonization of the skin and mucous membranes. Methods: In a retrospective analysis of 3082 patients who had undergone THA between 2014 and 2020, preoperative decolonization with octenidine dihydrochlorid was performed in the intervention group. In an interval of 30 days, soft tissue and prosthesis infections were detected, and an evaluation between the study groups was made by using a bilateral t-test regarding the presence of an early infection. The study groups were identical with regard to the ASA score, comorbidities, and risk factors. Results: Patients treated preoperatively with the octenidine dihydrochloride protocol showed lower early infection rates. In the group of intermediate- and high-risk patients (ASA 3 and higher), there was generally a significantly increased risk. The risk of wound or joint infection within 30 days was 1.99% higher for patients with ASA 3 or higher than for patients with standard care (4.11% [13/316] vs. 2.02% [10/494]; p = 0.08, relative risk 2.03). Preoperative decolonization shows no effect on the risk of infection that increases with age, and a gender-specific effect could not be detected. Looking at the body mass index, it could be shown that sacropenia or obesity leads to increased infection rates. Preoperative decolonization led to lower infection rates in percentage terms, which, however, did not prove to be significant (BMI < 20 1.98% [5/252] vs. 1.31% [5/382], relative risk 1.43, BMI > 30 2.58% [5/194] vs. 1.20% [4/334], relative risk 2.15). In the spectrum of patients with diabetes, it could be shown that preoperative decolonization leads to a significantly lower risk of infection (infections without protocol 18.3% (15/82), infections with protocol 8.50% (13/153), relative risk 2.15, p = 0.04. Conclusion: Preoperative decolonization appears to show a benefit, especially for the high-risk groups, despite the fact that in this patient group there is a high potential for resulting complications.
Penicillin-Susceptible Streptococcus pneumoniae Meningitis in Adults: Does the Ceftriaxone Dosing Matter?
Authors: Raemy Samuel , Casanova Carlo , Baldan Rossella , Barreto Erin , Tande Aaron J. , Endimiani Andrea , Leib Stephen L. , Fischer Urs , Sendi Parham
Published: May 2023

The recommended empiric ceftriaxone dosing regimen for acute bacterial meningitis in adults is 2 g every 12 h. After penicillin-susceptible Streptococcus pneumoniae is isolated as a causative microorganism, the ceftriaxone dose may be continued or reduced to a single dose of 2 g every 24 h, per institutional preference. There is no clear guidance that indicates the superiority of one regimen over the other. The objective of this study was to evaluate the susceptibility of S. pneumoniae in the cerebral spinal fluid (CSF) of patients with meningitis and the relationship between ceftriaxone dose and clinical outcomes. We identified 52 patients with S. pneumoniae meningitis with positive CSF cultures who were treated at the University Hospital, Bern, Switzerland, over a 19-year period. We collected clinical and microbiological data for evaluation. Broth microdilution and Etest methods were performed to test penicillin and ceftriaxone susceptibility. All isolates were susceptible to ceftriaxone. Ceftriaxone was empirically used in 50 patients, with a starting dosing regimen of 2 g every 24 h in 15 patients and 2 g every 12 h in 35 patients. In 32 patients started on a twice-daily regimen (91%), doses were reduced to once daily after a median of 1.5 (95% CI 1–2) days. The overall in-hospital mortality was 15.4% (n = 8), and 45.7% of patients reported at least one sequela of meningitis at the last follow-up (median 375, 95% CI 189–1585 days). We found no statistical difference in outcome between the 2 g every 24 h and the 2 g every 12 h ceftriaxone dosing regimens. A ceftriaxone total daily dose of 2 g may be associated with similar outcomes to a 4 g total daily dose, provided that the causative organism is highly susceptible to ceftriaxone. The persistence of neurological and infection sequelae at the last follow-up underscores the need for optimal treatment of these complex infections.
Tropospheric Delay Model Based on VMF and ERA5 Reanalysis Data
Authors: Zhang Mengtao , Wang Mengli , Guo Hang , Hu Junjun , Xiong Jian
Published: May 2023

The global tropospheric zenith delay grid products of VMF1 and VMF3 (Vienna mapping functions) with different resolutions are used to calculate the tropospheric zenith delay of eight IGS (International GNSS Service) stations in China, and the accuracy of the two products under different interpolation methods is analyzed. As a result, the accuracy of utilizing different interpolation methods shows no obvious differences. The interpolation accuracy of the VMF3 grid model is slightly higher than that of the VMF1, and the interpolation accuracy of tropospheric delay is related to the elevation difference of grid points. In addition, according to ERA5 (the fifth generation of the Global Climate Information Analysis data set), the atmospheric stratification tropospheric delay is obtained, and a ZTD (the zenith tropospheric delay) height change grid model is constructed using the least squares exponential fitting method. The accuracy of the model is verified using the tropospheric delay product provided by the IGS. Finally, the constructed ZTD height change grid model is used as ZTD height reduction to solve the problem of large tropospheric delay errors in the VMF interpolation when the height change is large. The model accuracy of URUM station improve from 96.47 mm.to 32.97 mm (65.82%).
Improved Adaptive Federated Kalman Filtering for INS/GNSS/VNS Integrated Navigation Algorithm
Authors: Wu Xuejia , Su Zhong , Li Lei , Bai Zekun
Published: May 2023

To address the issue of low positioning accuracy in unmanned vehicles navigating in obstructed spaces due to easily contaminated navigation measurement information, an improved adaptive federated Kalman filtering INS/GNSS/VNS integrated navigation algorithm is proposed. In this algorithm, an inertial navigation system (INS) serves as the common reference system, and, together with the global navigation satellite system (GNSS) and visual navigation system (VNS), they form the subsystems that together make up the main system. In the event of faulty measurement values in the subsystems, a combination of the residual chi-square and sliding-window averaging methods are used for fault detection to improve the fault tolerance of the integrated navigation algorithm. Additionally, an adaptive sharing factor is proposed to adjust the accuracy of the integrated navigation algorithm based on the accuracy of the sub-filters. Simulation experiments demonstrated that, compared with classic federated Kalman filtering, the proposed algorithm reduced the root mean square errors (RMSEs) of the three-dimensional position by 56.4%, 54.8%, and 43.4% and the root mean square errors of the three-dimensional velocity by 71.0%, 72.1%, and 28.4% in the event of sub-filter faults, effectively solving the problem of low positioning accuracy for unmanned vehicles in obstructed spaces while ensuring the real-time performance of the system.
Evaluating and Ranking SCPMS Enablers Using ISM and SWARA
Authors: Almakayeel Naif
Published: May 2023

The supply chain performance measurement system (SCPMS) is considered an integral and important part of supply chain management (SCM) for an effective and efficient supply chain (SC). The healthier and more flexible SCPMS is based on the enablers from which the SC metrics are taken. The identification of such enablers must be aligned with the strategic objectives of the organization and mapped to the SC measurement objectives. Hence, this study identifies a set of enablers and ranks them. Interpretive structural modeling (ISM)-based methodology is used in the current study to model the SCPMS implementation enablers. MICMAC analysis is further employed to categorize and comprehend the importance of each SCPMS enabler. ISM offers relationship modeling of SCPMS enablers, whereas MICMAC helps with classifying them into four categories. The Delphi method is then used to validate the ISM model. The SCPMS variable is also used in prioritization using stepwise weight assessment ratio analysis (SWARA). Practicing managers may benefit when developing and installing the SCPMS to satisfy the requirements of supply chain 4.0 for Industry 4.0 (I4.0). They will also be able to recognize and allocate resources while implementing SCPMS. The prioritization using SWARA provides an important ranking according to its importance. The SCPMS enablers of ‘top management support’, ‘SC 4.0 performance measurement awareness’, and ‘managerial readiness’ are found to be significant.
CDDO–HS: Child Drawing Development Optimization–Harmony Search Algorithm
Authors: Ameen Azad A. , Rashid Tarik A. , Askar Shavan
Published: May 2023

Child drawing development optimization (CDDO) is a recent example of a metaheuristic algorithm. The motive for inventing this method is children’s learning behavior and cognitive development, with the golden ratio being employed to optimize the aesthetic value of their artwork. Unfortunately, CDDO suffers from low performance in the exploration phase, and the local best solution stagnates. Harmony search (HS) is a highly competitive algorithm relative to other prevalent metaheuristic algorithms, as its exploration phase performance on unimodal benchmark functions is outstanding. Thus, to avoid these issues, we present CDDO–HS, a hybridization of both standards of CDDO and HS. The hybridized model proposed consists of two phases. Initially, the pattern size (PS) is relocated to the algorithm’s core and the initial pattern size is set to 80% of the total population size. Second, the standard harmony search (HS) is added to the pattern size (PS) for the exploration phase to enhance and update the solution after each iteration. Experiments are evaluated using two distinct standard benchmark functions, known as classical test functions, including 23 common functions and 10 CEC-C06 2019 functions. Additionally, the suggested CDDO–HS is compared to CDDO, the HS, and six others widely used algorithms. Using the Wilcoxon rank-sum test, the results indicate that CDDO–HS beats alternative algorithms.
Wear Model of Silicon Nitride Ceramic Balls in Three-Body Coupling Grinding Mode
Authors: Yu Wei , Lv Binghai , Yuan Julong
Published: May 2023

Silicon nitride ceramic balls are the key basic components of bearings in major equipment. Their key performance indices are accuracy and batch consistency. A grinding method with the most appropriate comprehensive performance is the basis and guarantee for optimizing these performance indices. In this study, an accurate wear model was established to predict the material removal rates (MRRs) of grinding methods and improve the dynamic grinding control ability of machinery during grinding, thus enabling the mass production of high-grade silicon nitride ceramic balls. A comprehensive analysis of various grinding and polishing methods revealed that the factors affecting sphericity were mainly manifested by the increase in ball sliding and the improvement in MRRs. More over, the three-body coupling grinding mode was considered as the grinding mode that was most applicable to silicon nitride ceramic balls. The upper disk served as an external nonlinear load, and the combination of the rotating speeds of the inner and outer disks of the lower grinding disk could actively control the ball’s angle of rotation. This three-body wear mode can fully envelop the grinding trajectory and ensure uniform grinding. The traditional two-body wear model was unsuitable for three-body coupling grinding. A wear model based on three-body wear was established to predict MRRs and understand the principle of material removal in the grinding of precision spheres. Theoretical analysis and experimental verification revealed that the MRRs of silicon nitride ceramic balls during wear are not only related to the process parameters of external load and speed but also to the physical properties and geometric parameters of balls, abrasives, and processing machinery. The wear model results of silicon nitride ceramic balls in the three-body coupling grinding mode can be obtained stably on the basis of the established wear model by removing adverse effects and adopting optimized processing parameters, thus verifying the correctness of the theoretical and simulation analyses.
A Photon Imaging Detector Model with High Resolution and High Counting Rate
Authors: Jiang Zhongzhi , Ni Qiliang
Published: May 2023

A cross strip (XS) anode detector is a photon-counting imaging detector with high spatial resolution. However, due to the Poisson distribution characteristics of the photons emitted by the target, photons with a small time interval will cause signal superposition and resolution degradation. This is particularly significant at a high photon count rate. The key link that restricts the counting rate of the XS detector is the electronic system. In this paper, we propose a new electronic signal processing system scheme using a digital trapezoidal shaping filter instead of a traditional Gaussian shaping filter, which enables the detector to maintain a high resolution at high count rates. In order to verify the feasibility of the scheme, the relationship between shaping errors and shaping parameters is studied. Furthermore, the relationship between spatial resolution and photon-counting rate at different noise levels is revealed by numerical simulation. The results show that the detector can achieve a spatial resolution of <50 μm at a photon count rate of >6 MHz for 1000 e RMS noise.
Improved YOLOv5-Based Lightweight Object Detection Algorithm for People with Visual Impairment to Detect Buses
Authors: Arifando Rio , Eto Shinji , Wada Chikamune
Published: May 2023

Object detection is crucial for individuals with visual impairment, especially when waiting for a bus. In this study, we propose a lightweight and highly accurate bus detection model based on an improved version of the YOLOv5 model. We propose integrating the GhostConv and C3Ghost Modules into the YOLOv5 network to reduce the number of parameters and floating-point operations per second (FLOPs), ensuring detection accuracy while reducing the model parameters. Following that, we added the SimSPPF module to replace the SPPF in the YOLOv5 backbone for increased computational efficiency and accurate object detection capabilities. Finally, we developed a Slim scale detection model by modifying the original YOLOv5 structure in order to make the model more efficient and faster, which is critical for real-time object detection applications. According to the experimental results, the Improved-YOLOv5 outperforms the original YOLOv5 in terms of the precision, recall, and mAP@0.5. Further analysis of the model complexity reveals that the Improved-YOLOv5 is more efficient due to fewer FLOPS, with fewer parameters, less memory usage, and faster inference time capabilities. The proposed model is smaller and more feasible to implement in resource-constrained mobile devices and a promising option for bus detection systems.
Effect of Traditional and Conservative Endodontic Access Cavities on Instrumentation Efficacy of Two Different Ni–Ti Systems: A Micro-CT Study
Authors: Koohnavard Mehrdad , Celikten Berkan , Buyuksungur Arda , Orhan Kaan
Published: May 2023

This study aims to compare the shaping efficiency of the nickel–titanium instrument systems, One Reci and ProTaper Ultimate, using micro-CT (micro-computed tomography) in traditional and conservative endodontic access cavities. The experimental groups were formed according to the type of access cavity and Ni–Ti file system to be used. Sixty mandibular molar teeth were randomly divided into two main groups, the conservative access cavity (CAC) group and the traditional access cavity (TAC) group, and randomly divided into two subgroups according to the file system. The groups were compared with a two-way ANOVA analysis in terms of volume change, surface area, non-instrumented area transportation, and thickness of the dentin in the danger zone area after root canal preparation. The groups showed no statistically significant differences in terms of volume change, surface area, or the thickness of the dentin in the danger zone area after root canal preparation (p > 0.05). However, in the percentage of non-instrumented areas post-instrumentation between groups, the percentage of non-instrumented areas was statistically higher in specimens with CAC compared to TAC (p < 0.05). Canal transportation was higher in CAC at all distances from the apical region (p < 0.05). Within the limitations of this study, CAC can also be used with some precautions as an alternative to TAC.
A Novel Passive Implantable Differential Mechanism to Restore Individuated Finger Flexion during Grasping following Tendon Transfer Surgery: A Pilot Study
Authors: Chakravarthi Raja Suraj , You Won Suk , Jalaleddini Kian , Casebier Justin C. , Lightdale-Miric Nina R. , Hentz Vincent R. , Valero-Cuevas Francisco J. , Balasubramanian Ravi
Published: May 2023

Tendon transfer surgery is often used to restore hand grasp function following high median-ulnar nerve palsy. This surgery typically reroutes and sutures the tendon of the extensor carpi radialis longus (ECRL) muscle to all four flexor digitorum profundus (FDP) tendons of the hand, coupling them together. This makes it difficult to grasp irregularly shaped objects. We propose inserting a novel implantable passive device between the FDP tendons to surgically construct a differential mechanism, enabling the fingers to individually adapt to the irregular contours during grasping. These passive implants with no moving parts are fabricated from biocompatible materials. We tested the implants’ ability to create differential flexion between the index and middle fingers when actuated by a single muscle in two human cadaver hands using a computerized closed-loop control paradigm. In these cadaveric models, the implants enabled significantly more differential flexion between the index and middle fingers for a wide range of donor tendon tensions. The implants also redistributed fingertip forces between fingers. When grasping uneven objects, the difference in contact forces between fingers reduced by nearly 23% compared to the current suture-based surgery. These results suggest that self-adaptive grasp is possible in tendon transfers that drive multiple distal flexor tendons.
Prospects for Combined Applications of Nanostructured Catalysts and Biocatalysts for Elimination of Hydrocarbon Pollutants
Authors: Maslova Olga , Senko Olga , Gladchenko Marina A. , Gaydamaka Sergey N. , Efremenko Elena
Published: May 2023

Due to the presence of environmental problems, it is urgent to improve the processes aimed at the processing and purification of hydrocarbon-containing wastes and wastewaters. The review presents the latest achievements in the development of nanostructured catalysts made from different materials that can be used to purify oil-polluted wastewaters (petroleum refinery wastewater, oilfield-produced water, sulfur-containing extracts from pre-oxidized crude oil and oil fractions, etc.) and eliminate components of hydrocarbon pollutants (polyaromatic hydrocarbons, phenols, etc.). The results of the analysis of possible combinations of chemical and biological catalysts for deeper and more effective solutions to the problems are discussed. The possibilities of highly efficient elimination of hydrocarbon pollutants as a result of the hybrid application of nanoparticles (graphene oxide, mesoporous silica, magnetic nanocatalysts, etc.) or catalytic nanocomposites for advanced oxidation processes and biocatalysts (enzymes, cells of bacteria, mycelial fungi, phototrophic microorganisms and natural or artificial microbial consortia) are analyzed.
Effect of Heat Treatment on the Cavitation Erosion Behavior of Nanocrystalline Surface Layer of 304 Stainless Steel
Authors: Huang Nengliang , Tian Ye , Yang Rui , Xiao Tonghu , Li Hua , Chen Xiuyong
Published: May 2023

In this study, a nanocrystalline layer composed primarily of martensite phase was prepared on the surface of 304 stainless steel. Furthermore, the martensite phase content in the nanocrystalline layer was adjusted by heat treatment at 500 °C and 550 °C, respectively, and the cavitation erosion resistance of the nanocrystalline layer before and after heat treatment was investigated. The results showed that the nanocrystalline layer before and after heat treatment exhibited excellent erosion resistance, with cumulative mass loss of approximately 1/7, 1/5, and 1/3 that of the traditional 304 stainless steel, respectively. The nanocrystalline layer could significantly inhibit the growth of cavitation pits due to the high density of grain boundaries. However, due to the decrease in hardness of the nanocrystalline layer after heat treatment, the propagation speed of cavitation cracks was accelerated, and the cavitation erosion performance of the nanocrystalline layer showed a downward trend.
Radiological Assessment of Extracranial Vertebral Artery Variations: A Computed Tomography Angiography Study
Authors: Al Hajri Faiza , Al Yahya’ey Bayan , Sirasanagandla Srinivasa Rao , Mogali Sreenivasulu Reddy , Al-Ajmi Eiman
Published: May 2023

We evaluated the incidence of intraforaminal and extraforaminal variations of the vertebral artery (VA) in the Omani population using computed tomography angiography (CTA). CTA results of 579 consecutive Omani patients (1158 VAs) were reviewed retrospectively for the analysis of unusual entrance transverse foramen (UE-V2), midline migration (MM), persistent first intersegmental artery (PFIA), and paracondylar process (PP) variations of VA. The anomalous origin and VA dominance were also determined. The sex and side differences in the incidence of these variations were analyzed using the Chi-square test. The incidence of UE-V2 was observed in 10.44% (121 out of 1158) of cases. The incidence of UE-V2 at different vertebral levels was observed in the sequence of C5 (71%) > C4 (20.7%) > C7 (7.43%) > C3 (0.8%). The incidence of MM variation was 1.29%. PFIA and PP variations were found in 0.17% and 0.60% of cases, respectively. Left-dominant VA was identified in 44.7% (259 out of 579) of subjects. The incidence of VA variations was not significantly associated with either sex or side. Anomalous VA origin from arch aorta (3.5%) and right common carotid artery (CCA) (0.08%) was identified. The incidence of UE-V2 in Omani subjects is comparatively higher than that reported in other Asian populations. A rare case of VA originating from the right CCA was also identified.
Surrogate Method for Suspended Sediment Concentration Monitoring on the Alluvial Reach of the River Danube (Baja, Hungary)
Authors: Vas László , Tamás Enikő Anna
Published: May 2023

Sediment balance is essential for understanding changes in river morphology and ecosystems and related services depending on them. However, the currently used methods to quantify riverine sediment processes are not adequate enough. We have examined the sediment regime of the Danube River, particularly the suspended sediment yield. This parameter can be calculated based on stage or discharge using a suspended sediment yield rating curve; however, the uncertainty of this method can reach even 150%. The suspended sediment yield of a section does not only depend on processes that take place in the riverbed; thus, it cannot be described by only one easily measurable parameter. An integrated surrogate method based on turbidity registration is tested in order to determine suspended sediment yield on the lower Hungarian (sand-bed alluvial) reach of the Danube River. The near-bank turbidity is converted into suspended sediment concentration and then into suspended sediment yield. The turbidity is measured with a built-in turbidity probe, while the suspended sediment yield is determined with traditional methods (discharge measurement, suspended sediment sampling, laboratory processing, and calculation). The traditional and integrated surrogate methods are compared based on the results of the measurements, and different aspect correlations are established between flow parameters, turbidity, and suspended load. The results achieved with the integrated method are promising, but more measurements are required in order to refine the relationships in a broader interval.
Verification of Automotive Monopost Seat Strength through Dynamic and Quasi-Static Simulations
Authors: Sun Di , Park Soojin , Han Yongtak , Kim Jinho
Published: May 2023

A monopost seat is a novel, lightweight seat developed to utilize most of the interior space of vehicles. The space under the seat can be utilized by inserting a monopost columnar structure between a monopost seat rail and the floorboard. This design increases the perceived spaciousness of the vehicle and imparts passengers with a higher degree of freedom, because the seats can be adjusted more easily than standard seats. Meeting these requirements will require the seats to be sufficiently strong and sturdy in order to maintain passenger safety. Compared to traditional seats, it is more likely that a monopost seat will shift under the mass of the passenger, and these seats are more vulnerable to external vehicle collisions. Passenger safety is further compromised if an operational function is added. Therefore, further research on monopost seats is required. This study performed quasi-static and dynamic simulations to determine if the attachment of monopost seats to a vehicle meets international safety requirements in collision events. With a focus on clarifying the dynamic simulation process, the outcomes of quasi-static simulations were compared with the results obtained from dynamic simulations.
Peculiarities of the Vertical Structure of Atmospheric Aerosol Fields in the Basin of Lake Baikal According to Lidar Observations
Authors: Nasonov Sergei , Balin Yurii , Klemasheva Marina , Kokhanenko Grigorii , Novoselov Mikhail , Penner Ioganes
Published: May 2023

The paper briefly describes some features of the formation of aerosol fields in the water area of Lake Baikal in summer. The experimental data were collected during long-term expeditionary studies on the southeastern coast of the lake, at the Boyarsky station (51.84° N, 106.06° E), with the use of a LOSA-M2 lidar designed at the Institute of Atmospheric Optics, Siberian Branch, Russian Academy of Sciences (IAO SB RAS). A complex, layered, vertical structure of the atmospheric aerosol in the mountain basin is revealed. This is caused by peculiarities of the temperature and wind regimes at this site. The general pattern of the calculated autocorrelation matrices shows a good correlation in the range of 0–1000 m (the correlation coefficient is greater than 0.5). The aerosol layers in the lower-kilometer layer are closely related to each other and have a common origin.
A Modified Simulated Annealing (MSA) Algorithm to Solve the Supplier Selection and Order Quantity Allocation Problem with Non-Linear Freight Rates
Authors: Gonzalez-Ayala Paulina , Alejo-Reyes Avelina , Cuevas Erik , Mendoza Abraham
Published: May 2023

Economic Order Quantity (EOQ) is an important optimization problem for inventory management with an impact on various industries; however, their mathematical models may be complex with non-convex, non-linear, and non-differentiable objective functions. Metaheuristic algorithms have emerged as powerful tools for solving complex optimization problems (including EOQ). They are iterative search techniques that can efficiently explore large solution spaces and obtain near-optimal solutions. Simulated Annealing (SA) is a widely used metaheuristic method able to avoid local suboptimal solutions. The traditional SA algorithm is based on a single agent, which may result in a low convergence rate for complex problems. This article proposes a modified multiple-agent (population-based) adaptive SA algorithm; the adaptive algorithm imposes a slight attraction of all agents to the current best solution. As a proof of concept, the proposed algorithm was tested on a particular EOQ problem (recently studied in the literature and interesting by itself) in which the objective function is non-linear, non-convex, and non-differentiable. With these new mechanisms, the algorithm allows for the exploration of different regions of the solution space and determines the global optimum in a faster manner. The analysis showed that the proposed algorithm performed well in finding good solutions in a reasonably short amount of time.
State-of-Health Estimation and Anomaly Detection in Li-Ion Batteries Based on a Novel Architecture with Machine Learning
Authors: Lee Junghwan , Sun Huanli , Liu Yuxia , Li Xue , Liu Yixin , Kim Myungjun
Published: May 2023

Variations across cells, modules, packs, and vehicles can cause significant errors in the state estimation of LIBs using machine learning algorithms, especially when trained with small datasets. Training with large datasets that account for all variations is often impractical due to resource and time constraints at initial product release. To address this issue, we proposed a novel architecture that leverages electronic control units, edge computers, and the cloud to detect unrevealed variations and abnormal degradations in LIBs. The architecture comprised a generalized deep neural network (DNN) for generalizability, a personalized DNN for accuracy within a vehicle, and a detector. We emphasized that a generalized DNN trained with small datasets must show reasonable estimation accuracy during cross validation, which is critical for real applications before online training. We demonstrated the feasibility of the architecture by conducting experiments on 65 DNN models, where we found distinct hyperparameter configurations. The results showed that the personalized DNN achieves a root mean square error (RMSE) of 0.33%, while the generalized DNN achieves an RMSE of 4.6%. Finally, the Mahalanobis distance was used to consider the SOH differences between the generalized DNN and personalized DNN to detect abnormal degradations.
Blockchain-Based Double-Layer Byzantine Fault Tolerance for Scalability Enhancement for Building Information Modeling Information Exchange
Authors: Suliyanti Widya Nita , Sari Riri Fitri
Published: May 2023

A Practical Byzantine Fault Tolerance (PBFT) is a consensus algorithm deployed in a consortium blockchain that connects a group of related participants. This type of blockchain suits the implementation of the Building Information Modeling (BIM) information exchange with few participants. However, when much more participants are involved in the BIM information exchange, the PBFT algorithm, which inherently requires intensive communications among participating nodes, has limitations in terms of scalability and performance. The proposed solution for a multi-layer BFT hypothesizes that multi-layer BFT reduces communication complexity. However, having more layers will introduce more latency. Therefore, in this paper, Double-Layer Byzantine Fault Tolerance (DLBFT) is proposed to improve the blockchain scalability and performance of BIM information exchange. This study shows a double-layer network structure of nodes that can be built with each node on the first layer, which connects and forms a group with several nodes on the second layer. This network runs the Byzantine Fault Tolerance algorithm to reach a consensus. Instead of having one node send messages to all the nodes in the peer-to-peer network, one node only sends messages to a limited number of nodes on Layer 1 and up to three nodes in each corresponding group in Layer 2 in a hierarchical network. The DLBFT algorithm has been shown to reduce the required number of messages exchanged among nodes by 84% and the time to reach a consensus by 70%, thus improving blockchain scalability. Further research is required if more than one party is involved in multi-BIM projects.
An Effective Chemical Permeabilization of Silkworm Embryos
Authors: Urbán-Duarte David , Tomita Shuichiro , Sakai Hiroki , Sezutsu Hideki , De La Torre-Sánchez José Fernando , Kainoh Yooichi , Furukawa Seiichi , Uchino Keiro
Published: May 2023

The lipid layer surrounding the vitelline membrane of insect eggs has a critical role in the waterproofing and desiccation resistance of embryos. However, this lipid layer also prevents the flux of chemicals into the embryos, such as cryoprotectants, which are required for successful cryopreservation. The permeabilization studies of silkworm embryos remain insufficient. Therefore, in this study, we developed a permeabilization method to remove the lipid layer in the silkworm, Bombyx mori, and examined factors affecting the viability of dechorionated embryos, including the types and exposure times of chemicals and embryonic stages. Among the chemicals used, hexane and heptane were effective for permeabilization, whereas Triton X-100 and Tween-80 were less effective. Regarding the embryonic stages, there were significant differences between 160 and 166 h after egg laying (AEL) at 25 °C. Consequently, we found that the treatment of 160 AEL embryos with hexane for 30 s was the best condition for the permeability and viability of embryos, in which over 62% of the permeabilized embryos grew up to the second larval instar and their moths could lay fertilized eggs. Our method can be used for various purposes, including permeability investigations using other chemicals and embryonic cryopreservation.
Secretory Expression and Application of Antilipopolysaccharide Factor 3 in Chlamydomonas reinhardtii
Authors: Ou Yaohui , Zhuang Huilin , Chen Ruoyu , Huang Danqiong , Wang Chaogang
Published: May 2023

Anti-lipopolysaccharide factor is a class of antimicrobial peptides with lipopolysaccharide-binding structural domains, which has a broad antimicrobial spectrum, high antimicrobial activities, and broad application prospects in terms of the aquaculture industry. However, the low yield of natural antimicrobial peptides and their poor expression activity in bacteria and yeast have hindered their exploration and utilization. Therefore, in this study, the extracellular expression system of Chlamydomonas reinhardtii, by fusing the target gene with the signal peptide, was used to express anti-lipopolysaccharide factor 3 (ALFPm3) from Penaeus monodon in order to obtain highly active ALFPm3. Transgenic C. reinhardtii T-JiA2, T-JiA3, T-JiA5, and T-JiA6, were verified using DNA-PCR, RT-PCR, and immunoblot. Additionally, the IBP1-ALFPm3 fusion protein could be detected not only within the cells but also in the culture supernatant. Moreover, the extracellular secretion containing ALFPm3 was collected from algal cultures, and then its bacterial inhibitory activity was analyzed. The results showed that the extracts from T-JiA3 had an inhibition rate of 97% against four common aquaculture pathogenic bacteria, including Vibrio harveyi, Vibrio anguillarum, Vibrio alginolyticus, and Vibrio parahaemolyticus. The highest inhibition rate of 116.18% was observed in the test against V. anguillarum. Finally, the minimum inhibition concentration (MIC) of the extracts from T-JiA3 to V. harveyi, V. anguillarum, V. alginolyticus, and V. parahaemolyticus were 0.11 μg/μL, 0.088 μg/μL, 0.11 μg/μL, and 0.011 μg/μL, respectively. This study supports the foundation of the expression of highly active anti-lipopolysaccharide factors using the extracellular expression system in C. reinhardtii, providing new ideas for the expression of highly active antimicrobial peptides.
Global Compressive Loading from an Ultra-Thin PEEK Button Augment Enhances Fibrocartilage Regeneration of Rotator Cuff Enthesis
Authors: Lin Chia-Wei , Chiang En-Rung , Chen Shih-Hao , Chen Poyu , Liu Heng-Jui , Chiu Joe Chih-Hao
Published: May 2023

A PEEK button is developed to improve the tendon-to-bone compression area. In total, 18 goats were divided into 12-week, 4-week, and 0-week groups. All underwent bilateral detachment of the infraspinatus tendon. In the 12-week group, 6 were fixed with a 0.8–1 mm-thick PEEK augment (A-12, Augmented), and 6 were fixed with the double-row technique (DR-12). Overall, 6 infraspinatus were fixed with PEEK augment (A-4) and without PEEK augment (DR-4) in the 4-week group. The same condition was performed in the 0-week groups (A-0 and DR-0). Mechanical testing, immunohistochemistry assessment, cell responses, tissue alternation, surgical impact, remodeling, and the expression of type I, II, and III collagen of the native tendon-to-bone insertion and new footprint areas were evaluated. The average maximum load in the A-12 group (393.75 (84.40) N) was significantly larger than in the TOE-12 group (229.17 (43.94) N) (p < 0.001). Cell responses and tissue alternations in the 4-week group were slight. The new footprint area of the A-4 group had better fibrocartilage maturation and more type III collagen expression than in DR-4 group. This result proved the novel device is safe and provides superior load-displacement to the double-row technique. There is a trend toward better fibrocartilage maturation and more collagen III secretions in the PEEK augmentation group.
Platelet Serotonin (5-HT) Concentration, Platelet Monoamine Oxidase B (MAO-B) Activity and HTR2A, HTR2C, and MAOB Gene Polymorphisms in Asthma
Authors: Konjevod Marcela , Sreter Katherina B. , Popovic-Grle Sanja , Lampalo Marina , Tudor Lucija , Jukic Irena , Nedic Erjavec Gordana , Bingulac-Popovic Jasna , Safic Stanic Hana , Nikolac Perkovic Matea , Markeljevic Jasenka , Samarzija Miroslav , Pivac Nela , Svob Strac Dubravka
Published: May 2023

The complex role of the serotonin system in respiratory function and inflammatory diseases such as asthma is unclear. Our study investigated platelet serotonin (5-HT) levels and platelet monoamine oxidase B (MAO-B) activity, as well as associations with HTR2A (rs6314; rs6313), HTR2C (rs3813929; rs518147), and MAOB (rs1799836; rs6651806) gene polymorphisms in 120 healthy individuals and 120 asthma patients of different severity and phenotypes. Platelet 5-HT concentration was significantly lower, while platelet MAO-B activity was considerably higher in asthma patients; however, they did not differ between patients with different asthma severity or phenotypes. Only the healthy subjects, but not the asthma patients, carrying the MAOB rs1799836 TT genotype had significantly lower platelet MAO-B activity than the C allele carriers. No significant differences in the frequency of the genotypes, alleles, or haplotypes for any of the investigated HTR2A, HTR2C and MAOB gene polymorphisms have been observed between asthma patients and healthy subjects or between patients with various asthma phenotypes. However, the carriers of the HTR2C rs518147 CC genotype or C allele were significantly less frequent in severe asthma patients than in the G allele carriers. Further studies are necessary to elucidate the involvement of the serotonergic system in asthma pathophysiology.
Evolution of the Immunoglobulin Isotypes—Variations of Biophysical Properties among Animal Classes
Authors: Pomarici Nancy D. , Cacciato Roberta , Kokot Janik , Fernández-Quintero Monica L. , Liedl Klaus R.
Published: May 2023

The adaptive immune system arose around 500 million years ago in jawed fish, and, since then, it has mediated the immune defense against pathogens in all vertebrates. Antibodies play a central role in the immune reaction, recognizing and attacking external invaders. During the evolutionary process, several immunoglobulin isotypes emerged, each having a characteristic structural organization and dedicated function. In this work, we investigate the evolution of the immunoglobulin isotypes, in order to highlight the relevant features that were preserved over time and the parts that, instead, mutated. The residues that are coupled in the evolution process are often involved in intra- or interdomain interactions, meaning that they are fundamental to maintaining the immunoglobulin fold and to ensuring interactions with other domains. The explosive growth of available sequences allows us to point out the evolutionary conserved residues and compare the biophysical properties among different animal classes and isotypes. Our study offers a general overview of the evolution of immunoglobulin isotypes and advances the knowledge of their characteristic biophysical properties, as a first step in guiding protein design from evolution.
Transmembrane Protein 175, a Lysosomal Ion Channel Related to Parkinson’s Disease
Authors: Tang Tuoxian , Jian Boshuo , Liu Zhenjiang
Published: May 2023

Lysosomes are membrane-bound organelles with an acidic lumen and are traditionally characterized as a recycling center in cells. Lysosomal ion channels are integral membrane proteins that form pores in lysosomal membranes and allow the influx and efflux of essential ions. Transmembrane protein 175 (TMEM175) is a unique lysosomal potassium channel that shares little sequence similarity with other potassium channels. It is found in bacteria, archaea, and animals. The prokaryotic TMEM175 consists of one six-transmembrane domain that adopts a tetrameric architecture, while the mammalian TMEM175 is comprised of two six-transmembrane domains that function as a dimer in lysosomal membranes. Previous studies have demonstrated that the lysosomal K+ conductance mediated by TMEM175 is critical for setting membrane potential, maintaining pH stability, and regulating lysosome–autophagosome fusion. AKT and B-cell lymphoma 2 regulate TMEM175’s channel activity through direct binding. Two recent studies reported that the human TMEM175 is also a proton-selective channel under normal lysosomal pH (4.5–5.5) as the K+ permeation dramatically decreased at low pH while the H+ current through TMEM175 greatly increased. Genome-wide association studies and functional studies in mouse models have established that TMEM175 is implicated in the pathogenesis of Parkinson’s disease, which sparks more research interests in this lysosomal channel.
The Role of Spermidine and Its Key Metabolites in Important, Pathogenic Human Viruses and in Parasitic Infections Caused by Plasmodium falciparum and Trypanosoma brucei
Authors: Kaiser Annette
Published: May 2023

The triamine spermidine is a key metabolite of the polyamine pathway. It plays a crucial role in many infectious diseases caused by viral or parasitic infections. Spermidine and its metabolizing enzymes, i.e., spermidine/spermine-N1-acetyltransferase, spermine oxidase, acetyl polyamine oxidase, and deoxyhypusine synthase, fulfill common functions during infection in parasitic protozoa and viruses which are obligate, intracellular parasites. The competition for this important polyamine between the infected host cell and the pathogen determines the severity of infection in disabling human parasites and pathogenic viruses. Here, we review the impact of spermidine and its metabolites in disease development of the most important, pathogenic human viruses such as SARS-CoV-2, HIV, Ebola, and in the human parasites Plasmodium and Trypanosomes. Moreover, state-of-the-art translational approaches to manipulate spermidine metabolism in the host and the pathogen are discussed to accelerate drug development against these threatful, infectious human diseases.
The Combination of Synoeca-MP Antimicrobial Peptide with IDR-1018 Stimulates Proliferation, Migration, and the Expression of Pro-Regenerative Genes in Both Human Skin Cell Cultures and 3D Skin Equivalents
Authors: Alencar-Silva Thuany , Díaz-Martín Rubén D. , Zonari Alessandra , Foyt Daniel , Guiang Mylieneth , Pogue Robert , Saldanha-Araujo Felipe , Dias Simoni Campos , Franco Octavio Luiz , Carvalho Juliana Lott
Published: May 2023

In skin lesions, the development of microbial infection affects the healing process, increasing morbidity and mortality rates in patients with severe burns, diabetic foot, and other types of skin injuries. Synoeca-MP is an antimicrobial peptide (AMP) that exhibits activity against several bacteria of clinical importance, but its cytotoxicity can represent a problem for its positioning as an effective antimicrobial compound. In contrast, the immunomodulatory peptide IDR-1018 presents low toxicity and a wide regenerative potential due to its ability to reduce apoptotic mRNA expression and promote skin cell proliferation. In the present study, we used human skin cells and a 3D skin equivalent models to analyze the potential of the IDR-1018 peptide to attenuate the cytotoxicity of synoeca-MP, as well as the influence of synoeca-MP/IDR-1018 combination on cell proliferation, regenerative processes, and wound repair. We found that the addition of IDR-1018 significantly improved the biological properties of synoeca-MP on skin cells without modifying its antibacterial activity against S. aureus. Likewise, in both melanocytes and keratinocytes, the treatment with synoeca-MP/IDR-1018 combination induces cell proliferation and migration, while in a 3D human skin equivalent model, it can accelerate wound reepithelization. Furthermore, treatment with this peptide combination generates an up-regulation in the expression of pro-regenerative genes in both monolayer cell cultures and in 3D skin equivalents. This data suggests that the synoeca-MP/IDR-1018 combination possesses a good profile of antimicrobial and pro-regenerative activity, opening the door to the development of new strategies for the treatment of skin lesions.
mef2ca and mef2cb Double Mutant Zebrafish Show Altered Craniofacial Phenotype and Motor Behaviour
Authors: Adrião Andreia , Mariano Sara , Mariano José , Gavaia Paulo J. , Cancela M. Leonor , Vitorino Marta , Conceição Natércia
Published: May 2023

The transcription factor MEF2C is crucial in neuronal, cardiac, bone and cartilage molecular processes, as well as for craniofacial development. MEF2C was associated with the human disease MRD20, whose patients show abnormal neuronal and craniofacial development. Zebrafish mef2ca;mef2cb double mutants were analysed for abnormalities in craniofacial and behaviour development through phenotypic analysis. Quantitative PCR was performed to investigate the expression levels of neuronal marker genes in mutant larvae. The motor behaviour was analysed by the swimming activity of 6 dpf larvae. We found that mef2ca;mef2cb double mutants display several abnormal phenotypes during early development, including those already described in zebrafish carrying mutations in each paralog, but also (i) a severe craniofacial phenotype (comprising both cartilaginous and dermal bone structures), (ii) developmental arrest due to the disruption of cardiac oedema and (iii) clear alterations in behaviour. We demonstrate that the defects observed in zebrafish mef2ca;mef2cb double mutants are similar to those previously described in MEF2C-null mice and MRD20 patients, confirming the usefulness of these mutant lines as a model for studies concerning MRD20 disease, the identification of new therapeutic targets and screening for possible rescue strategies.