bluuebunny/crossref_metadata_2025 huggingface dataset:
Hybrid Zero Dynamics Control for Gait Guidance of a Novel Adjustable Pediatric Lower-Limb Exoskeleton
Authors: Goo Anthony , Laubscher Curt A. , Wiebrecht Jason J. , Farris Ryan J. , Sawicki Jerzy T.
Published: May 2022

Exoskeleton technology has undergone significant developments for the adult population but is still lacking for the pediatric population. This paper presents the design of a hip–knee exoskeleton for children 6 to 11 years old with gait abnormalities. The actuators are housed in an adjustable exoskeleton frame where the thigh part can adjust in length and the hip cradle can adjust in the medial-lateral and posterior-anterior directions concurrently. Proper control of exoskeletons to follow nominal healthy gait patterns in a time-invariant manner is important for ease of use and user acceptance. In this paper, a hybrid zero dynamics (HZD) controller was designed for gait guidance by defining the zero dynamics manifold to resemble healthy gait patterns. HZD control utilizes a time-invariant feedback controller to create dynamically stable gaits in robotic systems with hybrid models containing both discrete and continuous dynamics. The effectiveness of the controller on the novel pediatric exoskeleton was demonstrated via simulation. The presented preliminary results suggest that HZD control provides a viable method to control the pediatric exoskeleton for gait guidance.
Lack of Ecto-5′-Nucleotidase Protects Sensitized Mice against Allergen Challenge
Authors: Caiazzo Elisabetta , Cerqua Ida , Turiello Roberta , Riemma Maria Antonietta , De Palma Giacomo , Ialenti Armando , Roviezzo Fiorentina , Morello Silvana , Cicala Carla
Published: May 2022

Ecto-5′-nucleotidase (CD73), the ectoenzyme that together with CD39 is responsible for extracellular ATP hydrolysis and adenosine accumulation, regulates immune/inflammatory processes by controlling innate and acquired immunity cell functions. We previously demonstrated that CD73 is required for the assessment of a controlled allergic sensitization, in mice. Here, we evaluated the response to aerosolized allergen of female-sensitized mice lacking CD73 in comparison with their wild type counterpart. Results obtained show, in mice lacking CD73, the absence of airway hyperreactivity in response to an allergen challenge, paralleled by reduced airway CD23+B cells and IL4+T cells pulmonary accumulation together with reduced mast cells accumulation and degranulation. Our findings indicate CD73 as a potential therapeutic target for allergic asthma.
Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives
Authors: Knežić Teodora , Janjušević Ljiljana , Djisalov Mila , Yodmuang Supansa , Gadjanski Ivana
Published: May 2022

Global food systems are under significant pressure to provide enough food, particularly protein-rich foods whose demand is on the rise in times of crisis and inflation, as presently existing due to post-COVID-19 pandemic effects and ongoing conflict in Ukraine and resulting in looming food insecurity, according to FAO. Cultivated meat (CM) and cultivated seafood (CS) are protein-rich alternatives for traditional meat and fish that are obtained via cellular agriculture (CA) i.e., tissue engineering for food applications. Stem and progenitor cells are the building blocks and starting point for any CA bioprocess. This review presents CA-relevant vertebrate cell types and procedures needed for their myogenic and adipogenic differentiation since muscle and fat tissue are the primary target tissues for CM/CS production. The review also describes existing challenges, such as a need for immortalized cell lines, or physical and biochemical parameters needed for enhanced meat/fat culture efficiency and ways to address them.
Descriptive Analysis of Circulating Antimicrobial Resistance Genes in Vancomycin-Resistant Enterococcus (VRE) during the COVID-19 Pandemic
Authors: Toc Dan Alexandru , Butiuc-Keul Anca Livia , Iordache Dumitrana , Botan Alexandru , Mihaila Razvan Marian , Costache Carmen Anca , Colosi Ioana Alina , Chiorean Claudia , Neagoe Dan Stefan , Gheorghiu Liana , Junie Lia Monica
Published: May 2022

COVID-19 offers ideal premises for bacteria to develop antimicrobial resistance. In this study, we evaluated the presence of several antimicrobial resistance genes (ARG) in vancomycin-resistant Enterococcus (VRE) isolated from rectal swabs from patients at a hospital in Cluj-Napoca, Romania. Rectal swabs were cultivated on CHROMID® VRE (bioMérieux, Marcy—l’ Étoile, France) and positive isolates were identified using MALDI-TOF Mass Spectrometry (Bruker Daltonics, Bremen, Germany) and further analyzed using the PCR technique for the presence of the following ARGs: van A, van B, tet(M), tet(L), ermB, msrA, mefA, aac(6′)-Im, aph(2)-Ib, ant(4′)-Ia, sul1, sul2, sul3, and NDM1. We isolated and identified 68 isolates of Enterococcus faecium and 11 isolates of Enterococcus faecalis. The molecular analysis showed 66 isolates positive for the vanA gene and eight positive for vanB. The most frequent association of ARG in VRE was vanA-tet(M)-ermB. There was no statistically significant difference between Enterococcus faecium and Enterococcus faecalis regarding ARGs. Our work proves that during the COVID-19 pandemic, highly resistant isolates of Enterococcus were present in patients in the intensive care unit; thus, better healthcare policies should be implemented for the management and control of these highly resistant isolates in the future.
A Review of the Recent Development in the Synthesis and Biological Evaluations of Pyrazole Derivatives
Authors: Ebenezer Oluwakemi , Shapi Michael , Tuszynski Jack A.
Published: May 2022

Pyrazoles are five-membered heterocyclic compounds that contain nitrogen. They are an important class of compounds for drug development; thus, they have attracted much attention. In the meantime, pyrazole derivatives have been synthesized as target structures and have demonstrated numerous biological activities such as antituberculosis, antimicrobial, antifungal, and anti-inflammatory. This review summarizes the results of published research on pyrazole derivatives synthesis and biological activities. The published research works on pyrazole derivatives synthesis and biological activities between January 2018 and December 2021 were retrieved from the Scopus database and reviewed accordingly.
Bio-Inspired Control System for Fingers Actuated by Multiple SMA Actuators
Authors: Uleru George-Iulian , Hulea Mircea , Burlacu Adrian
Published: May 2022

Spiking neural networks are able to control with high precision the rotation and force of single-joint robotic arms when shape memory alloy wires are used for actuation. Bio-inspired robotic arms such as anthropomorphic fingers include more junctions that are actuated simultaneously. Starting from the hypothesis that the motor cortex groups the control of multiple muscles into neural synergies, this work presents for the first time an SNN structure that is able to control a series of finger motions by activation of groups of neurons that drive the corresponding actuators in sequence. The initial motion starts when a command signal is received, while the subsequent ones are initiated based on the sensors’ output. In order to increase the biological plausibility of the control system, the finger is flexed and extended by four SMA wires connected to the phalanges as the main tendons. The results show that the artificial finger that is controlled by the SNN is able to smoothly perform several motions of the human index finger while the command signal is active. To evaluate the advantages of using SNN, we compared the finger behaviours when the SMA actuators are driven by SNN, and by a microcontroller, respectively. In addition, we designed an electronic circuit that models the sensor’s output in concordance with the SNN output.
Label-Free miRNA-21 Analysis Based on Strand Displacement and Terminal Deoxynucleotidyl Transferase-Assisted Amplification Strategy
Authors: Yan Ying , Zhao Han , Fang Yukang , Ma Changbei , Chen Junxiang
Published: May 2022

MicroRNAs (miRNAs) are regarded as a rising star in the biomedical industry. By monitoring slight increases in miRNA-21 levels, the possibilities of multi-type malignancy can be evaluated more precisely and earlier. However, the inconvenience and insensitivity of traditional methods for detecting miRNA-21 levels remains challenging. In this study, a partially complementary cDNA probe was designed to detect miRNA-21 with target-triggered dual amplification based on strand displacement amplification (SDA) and terminal deoxynucleotidyl transferase (TdT)-assisted amplification. In this system, the presence of miRNA-21 can hybridize with template DNA to initiate SDA, generating a large number of trigger molecules. With the assistance of TdT and dGTP, the released trigger DNA with 3′-OH terminal can be elongated to a superlong poly(guanine) sequence, and a notable fluorescence signal was observed in the presence of thioflavin T. By means of dual amplification strategy, the sensing platform showed a good response tomiRNA-21 with a detection limit of 1.7 pM (S/N = 3). Moreover, the specificity of this method was verified using a set of miRNA with sequence homologous to miRNA-21. In order to further explore its practical application capabilities, the expression of miRNA in different cell lines was quantitatively analyzed and compared with the qRT-PCR. The considerable results of this study suggest great potential for the application of the proposed approach in clinical diagnosis.
Two-Dimensional Ti3C2 MXene-Based Novel Nanocomposites for Breath Sensors for Early Detection of Diabetes Mellitus
Authors: Rudie Anna , Schornack Anna Marie , Wu Qiang , Zhang Qifeng , Wang Danling
Published: May 2022

The rates of diabetes throughout the world are rising rapidly, impacting nearly every country. New research is focused on better ways to monitor and treat this disease. Breath acetone levels have been defined as a biomarker for diabetes. The development of a method to monitor and diagnose diabetes utilizing breath acetone levels would provide a fast, easy, and non-invasive treatment option. An ideal material for point-of-care diabetes management would need to have a high response to acetone, high acetone selectivity, low interference from humidity, and be able to operate at room temperature. Chemiresistive gas sensors are a promising method for sensing breath acetone due to their simple fabrication and easy operation. Certain semiconductor materials in chemiresistive sensors can react to acetone in the air and produce changes in resistance that can be correlated with acetone levels. While these materials have been developed and show strong responses to acetone with good selectivity, most of them must operate at high temperatures (compared to RT), causing high power consumption, unstable device operation, and complex device design. In this paper, we systematically studied a series of 2-dimensional MXene-based nanocomposites as the sensing materials in chemiresistive sensors to detect 2.86 ppm of acetone at room temperature. Most of them showed great sensitivity and selectivity for acetone. In particular, the 1D/2D CrWO/Ti3C2 nanocomposite showed the best sensing response to acetone: nine times higher sensitivity than 1D KWO nanowires. To determine the sensing selectivity, a CrWO/Ti3C2 nanocomposite-based sensor was exposed to various common vapors in human breath. The result revealed that it has excellent selectivity for acetone, and far lower responses to other vapors. All these preliminary results indicate that this material is a promising candidate for the creation of a point-of-care diabetes management device.
Of Orchids and Dandelions: Empathy but Not Sensory Processing Sensitivity Is Associated with Tactile Discrimination Abilities
Authors: Schaefer Michael , Kevekordes Marie-Christin , Sommer Hanna , Gärtner Matti
Published: May 2022

Many concepts of the human personality are based on assumptions about underlying physiological processes. The most prominent example is probably the concept of extraversion introduced by H.J. Eysenck decades ago. However, more recent approaches also propose that personality traits may be reflected by physiological processes. For example, empathic personality dimensions have been linked to tactile perception, suggesting that individuals with higher tactile sensitivity are also more empathetic to the sensations of others. Another recent example is the concept of sensory processing sensitivity, which has been linked to enhanced primary sensory processing. However, the exact relationship between tactile abilities and personality is still unclear, thus the current study aims to test whether different personality dimensions affect the performance in a tactile acuity task. Tactile abilities of healthy participants were tested with tactile 2-point-thresholds on the hands. Personality dimensions were examined with respect to empathy, sensory processing sensitivity, and the Big Five. Results revealed that empathy, but not sensory processing sensitivity, was associated with tactile performance. We conclude that the ability to feel with someone else seems to be linked to the perception of our own body. Thus, the sense of touch may play an important role for empathy. We discuss explanations of these results and highlight possible implications of our findings.
Anxiety and Depression in Belgium during the First 15 Months of the COVID-19 Pandemic: A Longitudinal Study
Authors: Bruggeman Helena , Smith Pierre , Berete Finaba , Demarest Stefaan , Hermans Lize , Braekman Elise , Charafeddine Rana , Drieskens Sabine , De Ridder Karin , Gisle Lydia
Published: May 2022

The COVID-19 pandemic and policy measures enacted to contain the spread of the coronavirus have had nationwide psychological effects. This study aimed to assess the impact of the first 15 months of the COVID-19 pandemic on the level of anxiety (GAD-7 scale) and depression (PHQ-9 scale) of the Belgian adult population. A longitudinal study was conducted from April 2020 to June 2021, with 1838 respondents participating in 6 online surveys. Linear mixed models were used to model the associations between the predictor variables and the mental health outcomes. Results showed that the prevalence of symptoms of anxiety and depression was higher in times of stricter policy measures. Furthermore, after the initial stress from the outbreak, coping and adjustment were observed in participants, as symptoms of anxiety and depression decreased during times of lower policy restrictions to almost the same level as in pre-COVID times (2018). Though time trends were similar for all population subgroups, higher levels of both anxiety and depression were generally found among women, young people, people with poor social support, extraverts, people having pre-existing psychological problems, and people who were infected/exposed to the COVID-19 virus. Therefore, investment in mental health treatment programs and supports, especially for those risk groups, is crucial.
Compressive Behavior of a Fully Prefabricated Liftable Connection for Modular Steel Construction
Authors: Deng En-Feng , Lian Jun-Yi , Liu Zhe , Zhang Guang-Cao , Wang Shi-Bo , Cao Dian-Bin
Published: May 2022

Modular steel construction (MSC) consists of the off-site prefabrication of a fully finished module and the on-site assembling of the module unit. The popularity of MSC is on the rise, attributable to its technical advantages of speed and quality of buildings with repetitive units. Inter-module connection is critical for the overall stability and load-bearing capacity of MSC. An innovative, fully prefabricated liftable connection (FPLC) using standard corner fittings and long stay bolts is proposed in this paper. This paper focuses on the axial compressive behavior and design of FPLC. Five full-scale specimens were tested under axial compression. Local buckling of the column and shear of the long stay bolts were observed during the test. It can be concluded from the test results that the load-bearing capacity may decrease as the number and diameter of the stay bolts increase. A three-dimensional nonlinear finite element model (FEM) was developed and validated against the test results by general purpose finite element software ABAQUS. Furthermore, a parametric study was conducted using the verified FEM to provide a better understanding of the axial compressive behavior of the FPLC. The results of the parametric study indicated that the corner fitting can be up to 15% lighter for columns with thicknesses of 6 mm and 8 mm without substantial reduction of the axial load-bearing capacity of the FPLC. Moreover, the location of the column can be adjusted to achieve a uniform Von Mises stress and equivalent plastic strain (PEEQ) distribution of the connection. The presented research work provides an engineering-practical inter-module connection on its axial compressive behavior, which will provide helpful references for further application of MSC.
Prototyping a Lighting Control System Using LabVIEW with Real-Time High Dynamic Range Images (HDRis) as the Luminance Sensor
Authors: Budhiyanto Aris , Chiou Yun-Shang
Published: May 2022

Lighting control systems (LCSs) play important roles in maintaining visual comfort and energy savings in buildings. This paper presents a prototype LCS using LabVIEW with real-time high dynamic range images and a digital multiplex controller to brighten lamps sequentially to provide visual comfort. The prototype is applied to a scaled classroom model with three schemes involving different activities and needs: writing and reading, requiring a uniform luminance of approximately 100 cd/m2, teaching using a whiteboard, requiring an illuminance of approximately 120 cd/m2 for the whiteboard and 60 cd/m2 for the desks, and drawing and art activities focused on the center of the room, requiring an illuminance of approximately 100 cd/m2 for the center area and 50 cd/m2 for the background area. For each scheme, two conditions are presented: one in which the room is treated as a closed room without windows, and the one in which the room has a large window on one wall that enables daylight to penetrate the room. The prototype works well with both schemes and provides different combinations of lamp brightness levels, starting from 10% to 60%, based on the activities and required luminance, and can save around 73–82% of electricity. The presence of daylight does not always result in more energy savings, as the brightness contrast for visual comfort needs to be considered.
Catalytic Oxidation of Chlorobenzene over Ce-Mn-Ox/TiO2: Performance Study of the Porous Structure
Authors: Yang Bo , Ni Maosen , Gu Qiuxiang , Huang Qiong , Xu Leilei , Chen Mindong , Jin Qijie , Wang Zhenhui
Published: May 2022

Chlorobenzene (CB) is a volatile and harmful organic molecule that may result in deformities, cancer, etc. Catalytic oxidization of CB may be a way to manage it. The development of nonprecious catalysts with high catalytic activity is the key but is still a challenge. In this work, a series of Ce-Mn-Ox/TiO2 modified by citric acid monohydrate were developed and exhibited a composite pore structure. This pore structure leads to a large specific surface area, highly exposed activity sites, and excellent catalytic activity. The as-prepared 10C-CM/T exhibited nearly 100% efficiency for CB oxidization in the temperature range of 300–350 °C. The in situ DRIFT measurements demonstrated that the main intermediates at 250 °C are maleate and phenolic acid, whereas when the temperature is 350 °C, the main intermediates are carbonate, bidentate carbonate, and maleate.
Phage Display Affibodies Combined with AuNPs@Ru(bpy)32+ for Ultra-Sensitive Electrochemiluminescence Detection of Abrin
Authors: Liu Shuai , Tong Zhaoyang , Jiang Chunying , Gao Chuan , Xu Jianjie , Mu Xihui , Liu Bing , Du Bin , Liu Zhiwei , Zhang Pengjie
Published: May 2022

Abrin is a cytotoxin with strong lethality, which is a serious threat to human health and public safety, and thus, highly sensitive detection methods are urgently needed. The phage display affibody has two major modules, among which, the affibody fragment, with small molecular weight, high affinity and easy preparation, can be used for the specific recognition of the target, and the phage shell, with numerous protein copies, can be used as a carrier for the massive enrichment of signal molecules, and thus is particularly suitable as a sensitive probe for signal amplification in high-sensitivity biosensors. In this study, with antibody-coated magnetic microspheres as capture probes, Ru(bpy)32+ and biotin dual-labeled phage display affibodies as the specific signal probes and AuNPs@Ru(bpy)32+ (Ru(bpy)32+-coated gold nanoparticles) as the signal amplification nanomaterials, a new electrochemiluminescence (ECL) biosensor with a four-level sandwich structure of “magnetic capture probe-abrin-phage display affibody-AuNPs@Ru(bpy)32+” was constructed for abrin detection. In this detection mode, AuNPs@Ru(bpy)32+, a gold nanocomposite prepared rapidly via electrical interaction, contained an extremely high density of signal molecules, and the phage display affibodies with powerful loading capacity were not only labeled with Ru(bpy)32+, but also enriched with AuNPs@Ru(bpy)32+ in large amounts. These designs greatly improved the detection capability of the sensor, ultimately achieving the ultra-sensitive detection of abrin. The limit of detection (LOD) was 4.1 fg/mL (3δ/S), and the quantification range was from 5 fg/mL to 5 pg/mL. The sensor had good reproducibility and specificity and performed well in the test of simulated samples. This study expanded the application of affibodies in the field of biosensing and also deeply explored the signal amplification potential of phage display technology, which is of high value for the construction of simple and efficient sensors with high sensitivity.
Unmet Needs of Parents of Children with Urea Cycle Disorders
Authors: Scharping Mara , Brennenstuhl Heiko , Garbade Sven F. , Wild Beate , Posset Roland , Zielonka Matthias , Kölker Stefan , Haun Markus W. , Opladen Thomas
Published: May 2022

(1) Background: Phenotypic diversity and long-term health outcomes of individuals with urea cycle disorders (UCDs) have been described in detail. However, there is limited information on the burden on affected families. (2) Methods: To evaluate the family burden in parents with children suffering from UCDs, we used validated questionnaires. Socio-demographic characteristics were evaluated, and an adapted version of the Parental Need Scale for Rare Diseases questionnaire was used. The survey was conducted in families of UCD patients cared for at the University Children’s Hospital Heidelberg. (3) Results: From April to November 2021, 59 participants were interviewed (mothers n = 34, fathers n = 25). The affected patients most frequently suffered from ornithine transcarbamylase deficiency (OTC-D) (female n = 12, male n = 12), followed by argininosuccinate synthetase deficiency (ASS-D, n = 13) and argininosuccinate lyase deficiency (ASL-D, n = 8). About one-third of the participants were “dissatisfied” or “extremely dissatisfied” with health professionals’ disease knowledge. In addition, 30% of the participants reported a medium or high need for “additional information on the development of their children”, and 44% reported a medium or high need “for information on available services”. A majority of 68% reported a need for additional support regarding services such as support groups (42%) or psychological counseling (29%). (4) Conclusions: Our study indicates that there is an unmet need for sufficient information about the development of children with UCDs, as well as for information about available support services for families with UCD patients. Furthermore, the results highlight the importance of establishing or improving family-centered care approaches. This pilot study may serve as a template for the assessment of the family burden associated with other inherited metabolic diseases.
Difference in Hair Cortisol Concentrations between Obese and Non-Obese Children and Adolescents: A Systematic Review
Authors: Kitani Rosa Anna , Letsou Konstantina , Kokka Ioulia , Kanaka-Gantenbein Christina , Bacopoulou Flora
Published: May 2022

Childhood obesity has been linked to physical and psychological comorbidities that can be carried into adulthood. A bidirectional link between body weight and the stress system appears to exist, as cortisol may affect the regulation of appetite, while adiposity can affect cortisol secretion. Among the biological tissues used to evaluate cortisol concentrations, scalp hair can provide retrospective measures. The aim of this systematic review was to investigate the difference in hair cortisol concentrations between obese and non-obese minors ≤ 19 years of age. Children and adolescents with genetic, somatic or psychiatric comorbidities were excluded. The work was conducted following the PRISMA guidelines, using prespecified search terms in the Pubmed database. The initial search yielded 56 studies, while the last step of the screening procedure concluded in 9 observational studies. Among them, the results could be characterized as inconclusive. Five of them demonstrated significantly higher hair cortisol concentrations in obese children and adolescents than normal weight subjects. On the contrary, the remaining four found no statistically significant differences in hair cortisol concentrations between obese and non-obese subjects. Different methodologies applied, and confounding factors could explain the inconsistency in the findings. Further research is needed to provide more solid results.
Infantile-Onset Isolated Neurohypophyseal Langerhans Cell Histiocytosis with Central Diabetes Insipidus: A Case Report
Authors: Tani Mizuki , Hiroshima Shota , Sato Hidetoshi , Sawano Kentaro , Ogawa Yohei , Imamura Masaru , Oishi Makoto , Nagasaki Keisuke
Published: May 2022

Central diabetes insipidus (CDI) is a rare disease in children and has a variety of etiologies. The major causes of CDI with pituitary stalk thickening (PST) are germinoma, Langerhans cell histiocytosis (LCH), and Lymphocytic infundibulo-neurohypophysitis, which are difficult to differentiate by imaging and require pathological diagnosis. We report a case of infantile-onset isolated neurohypophyseal LCH diagnosed by pathological findings. A 2-year-old girl presented with polydipsia and polyuria. CDI was diagnosed and treatment with oral desmopressin was initiated. Magnetic resonance imaging (MRI) of the head showed PST and absence of high-signal intensity of posterior pituitary on T1-weighted images. Follow-up MRI scans showed that the tumor mass was gradually increasing and extending posteriorly toward the area near the mamillary body. Simultaneously, anterior pituitary dysfunction was observed. She underwent a biopsy of the PST and LCH was diagnosed by immunohistochemical analysis. DNA analysis showed no BRAF V600E mutation. Monotherapy with 2-Chlorodeoxyadenosine reduced the tumor size but did not improve pituitary function. Isolated neurohypophyseal LCH should be considered in infantile-onset cases of CDI with PST. 2-CdA treatment resulted in rapid PST shrinkage. Further cases are needed to determine whether early diagnosis and treatment can prevent anterior pituitary dysfunction.
Do Intellectually Gifted Children Show Similar Physical Habits, Physical Fitness Levels and Self-Perceived Body Image Than Typically Developed Children?
Authors: Otero Rodríguez Aroa , Sánchez-Lastra Miguel Adriano , Diz José Carlos , Ayán Carlos
Published: May 2022

Scientific evidence regarding whether intellectually gifted children show similar physical activity habits and physical fitness levels in comparison to typically developed children, is inconclusive. This is in part due to the scant research that has directly compared both groups of people. In this study, physical activity prevalence, self-perceived and objectively assessed physical fitness levels, and body image were assessed in a sample of 74 intellectually gifted children (mean age 11.6 ± 1.2 years). Seventy-four non-gifted children matched by age and sex were selected as a comparison cohort. Results indicated that both groups showed similar self-perceived and objectively assessed fitness levels. Physical activity habits were also similar, although significant differences were observed indicating that intellectually gifted girls were less active in comparison with non-gifted girls. Both cohorts perceived their body image accurately, although intellectually gifted children were much more satisfied with their physical appearance.
Sostdc1 Secreted from Cutaneous Lymphatic Vessels Acts as a Paracrine Factor for Hair Follicle Growth
Authors: Yoon Sun-Young , Detmar Michael
Published: May 2022

In our previous study, we found that lymphatic vessels stimulate hair follicle growth through paracrine effects on dermal papilla cells. However, the paracrine factors secreted from cutaneous lymphatic vessels that can activate dermal papilla cells are still unknown. In this study, we investigated whether lymphatic endothelial cells might secrete paracrine factors that activate dermal papilla cells in vitro. We found that Sostdc1 was more expressed in lymphatic endothelial cells compared with blood vascular endothelial cells. In addition, Sostdc1 expression levels were significantly increased during the anagen phase in the back skin of C57BL/6J mice, as compared to the telogen phase. We also observed that incubation of dermal papilla cells with 200 ng/mL Sostdc1 for 72 h induced the expression levels of Lef-1, a downstream target of Wnt signaling. Taken together, our results reveal that Sostdc1, a BMP antagonist, secreted from cutaneous lymphatic vessels, may act as a paracrine factor for hair follicle growth.
Whole-Transcriptome Profiling on Small FFPE Samples: Which Sequencing Kit Should Be Used?
Authors: Hilmi Marc , Armenoult Lucile , Ayadi Mira , Nicolle Rémy
Published: May 2022

RNA sequencing (RNA-Seq) appears as a great tool with huge clinical potential, particularly in oncology. However, sufficient sample size is often a limiting factor and the vast majority of samples from patients with cancer are formalin-fixed paraffin-embedded (FFPE). To date, several sequencing kits are proposed for FFPE samples yet no comparison on low quantities were performed. To select the most reliable, cost-effective, and relevant RNA-Seq approach, we applied five FFPE-compatible kits (based on 3′ capture, exome-capture and ribodepletion approaches) using 8 ng to 400 ng of FFPE-derived RNA and compared them to Nanostring on FFPE samples and to a reference PolyA (Truseq) approach on flash-frozen samples of the same tumors. We compared gene expression correlations and reproducibility. The Smarter Pico V3 ribodepletion approach appeared systematically the most comparable to Nanostring and Truseq (p < 0.001) and was a highly reproducible technique. In comparison with exome-capture and 3′ kits, the Smarter appeared more comparable to Truseq (p < 0.001). Overall, our results suggest that the Smarter is the most robust RNA-Seq technique to study small FFPE samples and 3′ Lexogen presents an interesting quality–price ratio for samples with less limiting quantities.
Enhanced Sewage Sludge Drying with a Modified Solar Greenhouse
Authors: Sorrenti Alice , Corsino Santo Fabio , Traina Francesco , Viviani Gaspare , Torregrossa Michele
Published: May 2022

This work reports the results obtained with an innovative configuration of a closed-static solar greenhouse for sludge drying. The novelty of the solar greenhouse configuration consisted in using a forced ventilation system to provide hot air for sludge drying and the utilization of solar irradiation for energy supply. Wet sewage sludge (97% humidity) was successfully dried up to a residual humidity close to 5% after 25 days during wintertime. The increase of the airflow rate supplied under the sludge bed improved the sludge drying rate. Moreover, the fraction of volatile suspended solids decreased from 70% to 41% after 13 days, indicating that air supply promoted the simultaneous stabilization of the sludge as a side-effect to the drying process. Overall, the specific energy consumption per ton of evaporated water was estimated to approximately 450 kWh/t, resulting in about 55% of energy demand lower than a conventional thermal drying system, while using only free solar energy. The achieved high weight reduction of up to 99% implies a noticeable reduction of the excess sludge handling costs, indicating that solar greenhouse drying is a highly interesting opportunity for sludge drying in medium-small sized WWTPs.
Wood Biochar Enhances the Valorisation of the Anaerobic Digestion of Chicken Manure
Authors: Ngo Tien , Khudur Leadin S. , Hakeem Ibrahim Gbolahan , Shah Kalpit , Surapaneni Aravind , Ball Andrew S.
Published: May 2022

In this study, the efficacy of biochar to mitigate ammonia stress and improve methane production is investigated. Chicken manure (CM) was subjected to high-solid mesophilic anaerobic digestion (15% total solid content) with wood biochar (BC). Wood biochar was further treated using HNO3 and NaOH to produce acid–alkali-treated wood biochar (TBC), with an improvement in its overall ammonium adsorption capacity and porosity. Three treatments were loaded in triplicate into the digesters, without biochar, with biochar and with acid–alkali-treated biochar and maintained at 37 °C for 110 days. The study found a significant improvement in CH4 formation kinetics via enhanced substrate degradation, leading to CH4 production of 74.7 mL g−1 VS and 70.1 mL g−1 VS by BC and TBC treatments, compared to 39.5 mL g−1 VS by control treatments on the 28th day, respectively. However, only the use of TBC was able to prolong methane production during the semi-inhibition phase. The use of TBC also resulted in the highest removal of total ammonia nitrogen (TAN) of 86.3%. In addition, the treatment with TBC preserved the highest microbial biomass at day 110. The presence of TBC also resulted in an increase in electrical conductivity, possibly promoting DIET-mediated methanogenesis. Overall, the acid–alkali treatment of biochar can be a novel approach to improve biochar’s existing characteristics for its utilisation as an additive in anaerobic digestion.
Traditional Water Governance Practices for Flood Mitigation in Ancient Sri Lanka
Authors: Hewawasam Vindya , Matsui Kenichi
Published: May 2022

The tank cascade system, which emerged as early as the fifth century BC in Sri Lanka’s dry zone, has been portrayed as one of the oldest water management practices in the world. However, its important function as flood management has not yet been thoroughly examined. In this paper, we argue that the main principle behind the tank cascade system is not only to recycle and reuse water resources by taking advantage of natural landscapes but also to control floods. This paper examines the evolution of traditional water management and flood mitigation techniques that flourished in pre-colonial Sri Lanka. This historical examination also sheds light on recent policies that exhibited renewed interests in revitalizing some aspects of the tank cascade system in Sri Lanka’s dry zone. This paper shows how ancient Sinhalese engineers and leaders incorporated traditional scientific and engineering knowledge into flood mitigation by engendering a series of innovations for land use planning, embankment designs, and water storage technologies. It also discusses how this system was governed by both kingdoms and local communities. Water management and flood control were among the highest priorities in urban planning and management. The paper thus discusses how, for centuries, local communities successfully sustained the tank cascade system through localized governance, which recent revitalized traditional water management projects often lack.
Interfacial Flows and Interfacial Shape Modulation Controlled by the Thermal Action of Light Energy
Authors: Ivanova Natalia
Published: May 2022

The review covers the research on thermocapillary convection caused by the thermal action of laser radiation in single-layer and bilayer liquid systems of capillary thickness. The advantages of using optical radiation are the instantaneous delivery of thermal energy to a place on demand (a bulk phase, interfaces); low radiation power required; concentrating heat flux on a spot of a few micrometers; the production of arbitrary spatial distributions of radiation intensity; and, as a result, corresponding thermal fields at a liquid interface and their fast reconfiguration. Thermocapillary stresses at the liquid interfaces lead to the transfer of the liquid and a change in the shape of the interface, in accordance with the distribution of the light-induced thermal field. Studies concerned with the methods of non-destructive testing of liquid media and solids, which are based on a photothermocapillary signal emitted by a laser-induced concave deformation of a thin layer, are considered. Features of thermocapillary deformation of a liquid–air interface caused by local heating of thin and thick (exceeding the capillary length) layers are demonstrated. A part of the review addresses the results of the study of thermocapillary rupture of films in the heating zone and the application of this effect in semiconductor electronics and high-resolution lithography. The works on the light-induced thermocapillary effect in bilayer (multilayer) liquid systems are analyzed, including early works on image recording liquid layer systems, liquid IR transducers, and nonlinear optical media.
Chaetarcturus cervicornis sp. n., a New Ross Sea Isopod of the Genus Chaetarcturus Brandt, 1990 (Crustacea, Malacostraca)
Authors: Noli Nicholas Francesco , Brandt Angelika , Di Franco Davide , Schiaparelli Stefano
Published: May 2022

In the framework of the PNRA (Italian National Antarctic Research Program) project CARBONANT focusing on biogenic carbonates and held in January–February 2002, several Ross Sea banks were sampled to obtain samples of biogenic carbonates. In the Mawson Bank, species belonging to the isopod genus Chaetarcturus Brandt, 1990 were recorded, including a specimen that did not match any described species. In this paper we describe Chaetarcturus cervicornis sp. n., which is characterized by supraocular spines and two pairs of tubercle-like protrusions on the cephalothorax. The new species is very similar to C. bovinus (Brandt & Wägele, 1988) and C. adareanus (Hodgson, 1902), but has a clearly different spine pattern. The study of the species of the genus Chaetarcturus in the Ross Sea contributes to increase our knowledge on the diversity of the Antarcturidae in the Southern Ocean. Ross Sea banks seem to hold an interesting and not-well-known fauna, deserving attention in future research.
Live Weight and Bone Growth from Birth to 23 Months of Age in Holstein–Friesian, Jersey and Crossbred Heifers
Authors: Gibson Michaela J. , Adams Brooke R. , Back Penny J. , Hickson Rebecca E. , Dittmer Keren E. , Rogers Chris W.
Published: May 2022

Selection pressure for greater milk solid production, calving ease and early puberty attainment has resulted in the alteration of the growth trajectory of New Zealand dairy heifers. Although previous data have looked at stature growth in terms of height and girth, changes in the growth trajectory as a result of seasonal growth has not been examined. In addition, the relative contributions of the appendicular skeleton and thorax in relation to height have not been considered in previous studies. Therefore, the aim of this study was to examine how the proportional contributions of stature change with increases in live weight in Holstein–Friesian, Jersey and Holstein–Friesian–Jersey crossbred heifers from birth to 23 months of age (just prior to calving). Comparisons of height, length, girth and distal and proximal forelimb length (stature) revealed that at the same live weight, measures of girth, length and height did not differ between breeds (p < 0.05). As live weight increases, the relative contribution of the distal limb to increasing height decreases, which is consistent with the cessation of physeal growth occurring earlier in the bones of the distal limb than in the proximal limb. By understanding the timing of proximal and distal growth in dairy heifers, the effect of seasonal growth on longitudinal bone growth can be identified.
The Coronavirus Disease 2019 Spatial Care Path: Home, Community, and Emergency Diagnostic Portals
Authors: Kost Gerald J.
Published: May 2022

This research uses mathematically derived visual logistics to interpret COVID-19 molecular and rapid antigen test (RAgT) performance, determine prevalence boundaries where risk exceeds expectations, and evaluate benefits of recursive testing along home, community, and emergency spatial care paths. Mathematica and open access software helped graph relationships, compare performance patterns, and perform recursive computations. Tiered sensitivity/specificity comprise: (T1) 90%/95%; (T2) 95%/97.5%; and (T3) 100%/≥99%, respectively. In emergency medicine, median RAgT performance peaks at 13.2% prevalence, then falls below T1, generating risky prevalence boundaries. RAgTs in pediatric ERs/EDs parallel this pattern with asymptomatic worse than symptomatic performance. In communities, RAgTs display large uncertainty with median prevalence boundary of 14.8% for 1/20 missed diagnoses, and at prevalence > 33.3–36.9% risk 10% false omissions for symptomatic subjects. Recursive testing improves home RAgT performance. Home molecular tests elevate performance above T1 but lack adequate validation. Widespread RAgT availability encourages self-testing. Asymptomatic RAgT and PCR-based saliva testing present the highest chance of missed diagnoses. Home testing twice, once just before mingling, and molecular-based self-testing, help avoid false omissions. Community and ER/ED RAgTs can identify contagiousness in low prevalence. Real-world trials of performance, cost-effectiveness, and public health impact could identify home molecular diagnostics as an optimal diagnostic portal.
The Use of Digital Pathology and Artificial Intelligence in Histopathological Diagnostic Assessment of Prostate Cancer: A Survey of Prostate Cancer UK Supporters
Authors: Rakovic Kai , Colling Richard , Browning Lisa , Dolton Monica , Horton Margaret R. , Protheroe Andrew , Lamb Alastair D. , Bryant Richard J. , Scheffer Richard , Crofts James , Stanislaus Ewart , Verrill Clare
Published: May 2022

There has been particular interest in the deployment of digital pathology (DP) and artificial intelligence (AI) in the diagnosis of prostate cancer, but little is known about the views of the public on their use. Prostate Cancer UK supporters were invited to an online survey which included quantitative and qualitative questions exploring views on the use of DP and AI in histopathological assessment. A total of 1276 responses to the survey were analysed (response rate 12.5%). Most respondents were supportive of DP (87%, 1113/1276) and of testing AI in clinical practice as a diagnostic adjunct (83%, 1058/1276). Respondents saw DP as potentially increasing workflow efficiency, facilitating research, education/training and fostering clinical discussions between clinician and patient. Some respondents raised concerns regarding data security, reliability and the need for human oversight. Among those who were unsure about AI, information was requested regarding its performance and others wanted to defer the decision to use it to an expert. Although most are in favour of its use, some are unsure, and their concerns could be addressed with more information or better communication. A small minority (<1%) are not in favour of the testing of the use of AI in histopathology for reasons which are not easily addressed.
Clinical Performance Comparing Titanium and Titanium–Zirconium or Zirconia Dental Implants: A Systematic Review of Randomized Controlled Trials
Authors: Fernandes Paulo , Otero Ada , Fernandes Juliana , Nassani Leonardo , Castilho Rogerio , de Oliveira Fernandes Gustavo
Published: May 2022

Objectives: This study aimed to compare clinical results between titanium (Ti), zirconia (Zr), or titanium–zirconium (TZ) dental implants and to analyze survival rate (SR), bleeding on probing (BoP), marginal bone loss (MBL), and/or probing depth (PD). Data source: Manual and electronic searches were conducted (PubMed and Web of Science) to identify randomized controlled trials that compared the outcomes of at least two implant types (control and test group) within the same study. The focused question was determined according to the PICOT strategy. Seven studies were included out of 202 research studies initially found. The follow-up periods ranged from 12 to 80 months, and the mean age was from 43.3 to 65.8 years old. The SR for Ti, TZ, and Zr implants ranged from 92.6% to 100%, 95.8% to 100%, and 87.5% to 91.25%, respectively; MBL for Ti, TZ, and Zr implants varied from −1.17 mm to −0.125 mm for Ti, −0.6 mm to −0.32 mm for TZ, and −0.25 mm to −1.38 mm for Zr. Studies showed a low incidence of mucositis and peri-implantitis; however, BoP for Zr was 16.43%, Ti ranged between 10% and 20%, and TZ from 10% to 13.8%. PD for Ti ranged from 1.6 mm to 3.05 mm, TZ was 3.12 mm (only one study), and Zr ranged from 2.21 mm to 2.6 mm. Conclusion: All three types of implants showed similar tissue behavior. However, the TZ group had better results when compared with Ti and Zr for SR, MBL, and BoP, except for PD. Furthermore, the worst SR was found in the Zr implants group.
On the Role of Dietary Nitrate in the Maintenance of Systemic and Oral Health
Authors: Schlagenhauf Ulrich
Published: May 2022

The assessment of the significance of nitrates ingested with food has undergone a fundamental change in recent years after many controversial discussions. While for a long time, a diet as low in nitrates as possible was advocated on the basis of epidemiological data suggesting a cancer-promoting effect of nitrate-rich diets, more recent findings show that dietary nitrate, after its conversion to nitrite by nitrate-reducing bacteria of the oral microbiota, is an indispensable alternative source for the formation of nitric oxide (NO), which comprises a key element in the physiology of a variety of central body functions such as blood pressure control, defense against invading bacteria and maintenance of a eubiotic microbiota in the gut and oral cavity. This compact narrative review aims to present the evidence supported by clinical and in vitro studies on the ambivalent nature of dietary nitrates for general and oral health and to explain how the targeted adjuvant use of nitrate-rich diets could open new opportunities for a more cause-related control of caries and periodontal disease.
Correction: Brochet et al. A Quantitative Comparison between Shannon and Tsallis–Havrda–Charvat Entropies Applied to Cancer Outcome Prediction. Entropy 2022, 24, 436
Authors: Brochet Thibaud , Lapuyade-Lahorgue Jérôme , Huat Alexandre , Thureau Sébastien , Pasquier David , Gardin Isabelle , Modzelewski Romain , Gibon David , Thariat Juliette , Grégoire Vincent , Vera Pierre , Ruan Su
Published: May 2022

Alexandre Huat, Sébastien Thureau, David Pasquier, Isabelle Gardin, Romain Modzelewski, David Gibon, Juliette Thariat and Vincent Grégoire were not included as authors in the original publication [...]