bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuroanatomy of Patients with Deficit Schizophrenia: An Exploratory Quantitative Meta-Analysis of Structural Neuroimaging Studies
Authors: Chee Tji Tjian , Chua Louis , Morrin Hamilton , Lim Mao Fong , Fam Johnson , Ho Roger
Published: August 2020

Little is known regarding the neuroanatomical correlates of patients with deficit schizophrenia or persistent negative symptoms. In this meta-analysis, we aimed to determine whether patients with deficit schizophrenia have characteristic brain abnormalities. We searched PubMed, CINAHL and Ovid to identify studies that examined the various regions of interest amongst patients with deficit schizophrenia, patients with non-deficit schizophrenia and healthy controls. A total of 24 studies met our inclusion criteria. A random-effects model was used to calculate a combination of outcome measures, and heterogeneity was assessed by the I2 statistic and Cochran’s Q statistic. Our findings suggested that there was statistically significant reduction in grey matter volume (−0.433, 95% confidence interval (CI): −0.853 to −0.014, p = 0.043) and white matter volume (−0.319, 95% CI: −0.619 to −0.018, p = 0.038) in patients with deficit schizophrenia compared to healthy controls. There is also statistically significant reduction in total brain volume (−0.212, 95% CI: −0.384 to −0.041, p = 0.015) and white matter volume (−0.283, 95% CI: −0.546 to −0.021, p = 0.034) in patients with non-deficit schizophrenia compared to healthy controls. Between patients with deficit and non-deficit schizophrenia, there were no statistically significant differences in volumetric findings across the various regions of interest.
South Indian Cuisine with Low Glycemic Index Ingredients Reduces Cardiovascular Risk Factors in Subjects with Type 2 Diabetes
Authors: Pavithran Nivedita , Kumar Harish , Menon Arun Somasekharan , Pillai Gopala Krishna , Sundaram Karimassery Ramaiyer , Ojo Omorogieva
Published: August 2020

Background: Inflammation is considered as a predictor of cardiovascular diseases in type 2 diabetes mellitus. No previous studies have investigated the effect of low glycemic index (LGI) recipes of South Indian cuisine on the risk factors of cardiovascular disease in patients with diabetes. Aim: The aim of this randomized controlled trial was to evaluate the improvement in cardiovascular risk factors and blood glucose control, in patients with type 2 diabetes, after intervention with recipes of Kerala cuisine, from locally available whole grain cereals, low in glycemic index. Method: This was a prospective and randomized controlled study that was conducted over a period of 24 weeks. A total of 80 participants were recruited from the Department of Endocrinology and Diabetes Outpatient in Kerala, South India. All 80 patients had type 2 diabetes, and were aged between 35 and 65 years. Participants were randomly assigned and advised to follow either a LGI diet plan (n = 40) or their usual diet, which served as a control group (n = 40). The advice was reinforced throughout the study period. Anthropometric, biochemical parameters which included glycemic and cardio-metabolic parameters were measured according to standard procedures. T-tests were conducted to compare the differences between intervention and control groups, and the Pearson correlation coefficient was used to evaluate associations between the variables. Results: There were significant differences (p < 0.05) between the intervention and control groups with respect to weight, HbA1c, insulin, triglycerides, Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), high sensitivity C-reactive protein (hs-CRP) and apolipoprotein B (ApoB). There was also a positive correlation between weight and blood glucose variables. ApoB was positively correlated with lipid profile and insulin levels. Conclusions: The long-term implementation of LGI diet of Kerala cuisine has been found to promote weight loss, enhance insulin sensitivity and reduce the cardiovascular risk.
Effectiveness of Social Measures against COVID-19 Outbreaks in Selected Japanese Regions Analyzed by System Dynamic Modeling
Authors: Niwa Makoto , Hara Yasushi , Sengoku Shintaro , Kodama Kota
Published: August 2020

In Japan’s response to the coronavirus disease 2019 (COVID-19), virus testing was limited to symptomatic patients due to limited capacity, resulting in uncertainty regarding the spread of infection and the appropriateness of countermeasures. System dynamic modelling, comprised of stock flow and infection modelling, was used to describe regional population dynamics and estimate assumed region-specific transmission rates. The estimated regional transmission rates were then mapped against actual patient data throughout the course of the interventions. This modelling, together with simulation studies, demonstrated the effectiveness of inbound traveler quarantine and resident self-isolation policies and practices. A causal loop approach was taken to link societal factors to infection control measures. This causal loop modelling suggested that the only effective measure against COVID-19 transmission in the Japanese context was intervention in the early stages of the outbreak by national and regional governments, and no social self-strengthening dynamics were demonstrated. These findings may contribute to an understanding of how social resilience to future infectious disease threats can be developed.
Attitudes and Consumer Behavior toward Foods Offered in Staff Canteens
Authors: Czarniecka-Skubina Ewa , Górska-Warsewicz Hanna , Trafiałek Joanna
Published: August 2020

The aim of our study was to analyze the attitudes of employees toward food offered in staff canteens, to analyze their eating behavior and the factors determining the choice of meals in staff canteens. The survey was conducted on a sample of 600 adult respondents, who patronize staff canteens in Warsaw, Poland. The research enabled a detailed and comprehensive assessment of consumer behavior toward the use of staff canteens, as well as their opinions on the functioning of the canteens and meals offered. Factors determining the frequency of use of canteens, type of meals, and factors influencing the use of such establishments were identified. Respondents assessed the quality and variety of meals, with the size of portion being the most valued. They were satisfied with the functioning of the canteens and had very few comments. However, they pointed out the need for an increase in the variety of meals, including the availability of vegetarian and vegan dishes. Our research enabled us to establish consumer profiles based on the reasons for not using staff canteens and comments about staff canteens. Patrons of staff canteens were referred to as “canteen enthusiasts” or “canteen medium-enthusiasts”, who are “snack lovers”, as well as “food choice-oriented” or “quality-oriented”. The results provide the basis for practical implications for owners or managers of staff canteens, part of whose work it is to analyze the needs and expectations of their potential customers.
Professional Master’s Degree Students’ Perceptions on the Changes Digitalisation Imposes on Counselling in the Social and Health Care Sector
Authors: Silvennoinen Piia
Published: August 2020

The study portrays the social and health care master’s students’ (N = 19) perceptions on the changes in work practices due to digitalisation, with a special focus on online counselling. Furthermore, the data include thoughts on the impact digitalised interaction, i.e., online counselling, has on their work and professional identities. The students studied how the motivational interviewing method combined with a solution-focused counselling approach is applicable in online settings by using simulation pedagogy. The data consisted of students’ learning diaries. Furthermore, the data were analysed using inductive content analysis. The results show that the digitalisation of work practices demands blended professionalism, which allows the professional to work both face-to-face and online with clients. In addition, the education of social and health care professionals needs to address the lack of competences and skills in digitalised work practices and provide a conceptual and practical understanding of blended professionalism in the sector.
Development and Implementation of Societal Influences Survey Questionnaire (SISQ) for Peoples during COVID-19 Pandemic: A Validity and Reliability Analysis
Authors: Li Dian-Jeng , Kao Wei-Tsung , Shieh Vincent , Chou Frank Huang-Chih , Lo Huei-Wen Angela
Published: August 2020

The emergence of Coronavirus disease 2019 (COVID-19) had rapidly spread since FEB/MAR 2020. Policy to prevent transmission of COVDI-19 resulted in multi-dimensional impact on social interaction. We aimed to develop a beneficial survey tool with favorable quality and availability, the Societal Influences Survey Questionnaire (SISQ), to evaluate social influences on people during this pandemic. The SISQ was developed with 15 items and 4-point Likert scales consisting of five factors. These include social distance, social anxiety, social desirability, social information, and social adaptation. Construct validity and reliability were performed to verify the SISQ. A total of 1912 Taiwanese were recruited. The results demonstrated that the SISQ has acceptable reliability, with Cronbach’s alphas ranging between 0.57 and 0.76. The SISQ accounted for 58.86% and satisfied the requirement of Kaiser–Mayer–Olkinvalues (0.78) and significant Bartlett’s Test of sphericity. Moreover, the confirmatory factor analysis fit indices also indicated the adequacy of the model. As for multiple comparison, females scored higher than males in factor of social distance. Unemployed participants and those without partners scored higher in several domains of factors. The survey method and survey instrument prove reliable and valuable, also providing different categories of assessment results regarding social influences and their impacts. Further studies are warranted to extend the applicability of SISQ.
Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro
Authors: Labedz-Maslowska Anna , Bryniarska Natalia , Kubiak Andrzej , Kaczmarzyk Tomasz , Sekula-Stryjewska Malgorzata , Noga Sylwia , Boruczkowski Dariusz , Madeja Zbigniew , Zuba-Surma Ewa
Published: August 2020

Human dental pulp harbours unique stem cell population exhibiting mesenchymal stem/stromal cell (MSC) characteristics. This study aimed to analyse the differentiation potential and other essential functional and morphological features of dental pulp stem cells (DPSCs) in comparison with Wharton’s jelly-derived MSCs from the umbilical cord (UC-MSCs), and to evaluate the osteogenic differentiation of DPSCs in 3D culture with a hypoxic microenvironment resembling the stem cell niche. Human DPSCs as well as UC-MSCs were isolated from primary human tissues and were subjected to a series of experiments. We established a multiantigenic profile of DPSCs with CD45−/CD14−/CD34−/CD29+/CD44+/CD73+/CD90+/CD105+/Stro-1+/HLA-DR− (using flow cytometry) and confirmed their tri-lineage osteogenic, chondrogenic, and adipogenic differentiation potential (using qRT-PCR and histochemical staining) in comparison with the UC-MSCs. The results also demonstrated the potency of DPSCs to differentiate into osteoblasts in vitro. Moreover, we showed that the DPSCs exhibit limited cardiomyogenic and endothelial differentiation potential. Decreased proliferation and metabolic activity as well as increased osteogenic differentiation of DPSCs in vitro, attributed to 3D cell encapsulation and low oxygen concentration, were also observed. DPSCs exhibiting elevated osteogenic potential may serve as potential candidates for a cell-based product for advanced therapy, particularly for bone repair. Novel tissue engineering approaches combining DPSCs, 3D biomaterial scaffolds, and other stimulating chemical factors may represent innovative strategies for pro-regenerative therapies.
“Clicking” an Ionic Liquid to a Potent Antimicrobial Peptide: On the Route towards Improved Stability
Authors: Gomes Ana , Bessa Lucinda J. , Correia Patrícia , Fernandes Iva , Ferraz Ricardo , Gameiro Paula , Teixeira Cátia , Gomes Paula
Published: August 2020

A covalent conjugate between an antibacterial ionic liquid and an antimicrobial peptide was produced via “click” chemistry, and found to retain the parent peptide’s activity against multidrug-resistant clinical isolates of Gram-negative bacteria, and antibiofilm action on a resistant clinical isolate of Klebsiella pneumoniae, while exhibiting much improved stability towards tyrosinase-mediated modifications. This unprecedented communication is a prelude for the promise held by ionic liquids -based approaches as tools to improve the action of bioactive peptides.
Tumor Suppressor Role of hsa-miR-193a-3p and -5p in Cutaneous Melanoma
Authors: Polini Beatrice , Carpi Sara , Doccini Stefano , Citi Valentina , Martelli Alma , Feola Sara , Santorelli Filippo Maria , Cerullo Vincenzo , Romanini Antonella , Nieri Paola
Published: August 2020

Background: Remarkable deregulation of several microRNAs (miRNAs) is demonstrated in cutaneous melanoma. hsa-miR-193a-3p is reported to be under-expressed in tissues and in plasma of melanoma patients, but the role of both miR-193a arms in melanoma is not known yet. Methods: After observing the reduced levels of miR-193a arms in plasma exosomes of melanoma patients, the effects of hsa-miR-193a-3p and –5p transfection in cutaneous melanoma cell lines are investigated. Results: In melanoma cell lines A375, 501Mel, and MeWo, the ectopic over-expression of miR-193a arms significantly reduced cell viability as well as the expression of genes involved in proliferation (ERBB2, KRAS, PIK3R3, and MTOR) and apoptosis (MCL1 and NUSAP1). These functional features were accompanied by a significant downregulation of Akt and Erk pathways and a strong increase in the apoptotic process. Since in silico databases revealed TROY, an orphan member of the tumor necrosis receptor family, as a potential direct target of miR-193a-5p, this possibility was investigated using the luciferase assay and excluded by our results. Conclusions: Our results underline a relevant role of miR-193a, both -3p and -5p, as tumor suppressors clarifying the intracellular mechanisms involved and suggesting that their ectopic over-expression could represent a novel treatment for cutaneous melanoma patients.
In Silico Finding of Key Interaction Mediated α3β4 and α7 Nicotinic Acetylcholine Receptor Ligand Selectivity of Quinuclidine-Triazole Chemotype
Authors: Arunrungvichian Kuntarat , Chongruchiroj Sumet , Sarasamkan Jiradanai , Schüürmann Gerrit , Brust Peter , Vajragupta Opa
Published: August 2020

The selective binding of six (S)-quinuclidine-triazoles and their (R)-enantiomers to nicotinic acetylcholine receptor (nAChR) subtypes α3β4 and α7, respectively, were analyzed by in silico docking to provide the insight into the molecular basis for the observed stereospecific subtype discrimination. Homology modeling followed by molecular docking and molecular dynamics (MD) simulations revealed that unique amino acid residues in the complementary subunits of the nAChR subtypes are involved in subtype-specific selectivity profiles. In the complementary β4-subunit of the α3β4 nAChR binding pocket, non-conserved AspB173 through a salt bridge was found to be the key determinant for the α3β4 selectivity of the quinuclidine-triazole chemotype, explaining the 47–327-fold affinity of the (S)-enantiomers as compared to their (R)-enantiomer counterparts. Regarding the α7 nAChR subtype, the amino acids promoting a however significantly lower preference for the (R)-enantiomers were the conserved TyrA93, TrpA149 and TrpB55 residues. The non-conserved amino acid residue in the complementary subunit of nAChR subtypes appeared to play a significant role for the nAChR subtype-selective binding, particularly at the heteropentameric subtype, whereas the conserved amino acid residues in both principal and complementary subunits are essential for ligand potency and efficacy.
In Vivo Optical Reporter-Gene-Based Imaging of Macrophage Infiltration of DNCB-Induced Atopic Dermatitis
Authors: Lee Sang Bong , Park Hyeonsoo , Lee Jae-Eon , Kim Kil-Soo , Jeon Yong Hyun
Published: August 2020

This study was conducted to monitor the macrophage infiltration of atopic dermatitis (AD)-like skin lesions and to evaluate the effects of anti-AD therapeutic agents in immunocompetent mice via optical reporter-gene-based molecular imaging. The enhanced firefly luciferase (effluc)-expressing macrophage cell line (Raw264.7/effluc) was intravenously introduced into mice with 2,4-dinitrochlorobenzene (DNCB)-induced AD, followed by bioluminescent imaging (BLI). After in vivo imaging, AD-like skin lesions were excised, and ex vivo imaging and Western blotting were conducted to determine the presence of infused macrophages. Finally, the therapeutic effect of dexamethasone (DEX), an AD-modulating agent, was evaluated via macrophage tracking. In vivo imaging with BLI revealed the migration of the reporter macrophages to DNCB-induced AD-like skin lesions on day 1 post-transfer. The greatest recruitment was observed on day 3, and a decline in BLI signal was observed on day 14. Notably, in vivo BLI clearly showed the inhibition of the reporter macrophage infiltration of DNCB-induced AD-like skin lesions by DEX, which was consistent with the reduced AD symptoms observed in DEX-treated mice. We successfully visualized the macrophage migration to DNCB-induced AD-like skin lesions, proving the feasibility of macrophage imaging for evaluating AD-regulating drugs in living organisms.
Phototaxis of the Unicellular Red Alga Cyanidioschyzon merolae Is Mediated by Novel Actin-Driven Tentacles
Authors: Maschmann Sascha , Ruban Karin , Wientapper Johanna , Walter Wilhelm J.
Published: August 2020

Phototaxis, which is the ability to move towards or away from a light source autonomously, is a common mechanism of unicellular algae. It evolved multiple times independently in different plant lineages. As of yet, algal phototaxis has been linked mainly to the presence of cilia, the only known locomotive organelle in unicellular algae. Red algae (Rhodophyta), however, lack cilia in all stages of their life cycle. Remarkably, multiple unicellular red algae like the extremophile Cyanidioschyzon merolae (C. merolae) can move towards light. Remarkably, it has remained unclear how C. merolae achieves movement, and the presence of a completely new mechanism has been suggested. Here we show that the basis of this movement are novel retractable projections, termed tentacles due to their distinct morphology. These tentacles could be reproducibly induced within 20 min by increasing the salt concentration of the culture medium. Electron microscopy revealed filamentous structures inside the tentacles that we identified to be actin filaments. This is surprising as C. merolae’s single actin gene was previously published to not be expressed. Based on our findings, we propose a model for C. merolae’s actin-driven but myosin-independent motility. To our knowledge, the described tentacles represent a novel motility mechanism.
Sub-Lethal Effects of Partially Purified Protein Extracted from Beauveria bassiana (Balsamo) and Its Presumptive Role in Tomato (Lycopersicon esculentum L.) Defense against Whitefly (Bemisia tabaci Genn.)
Authors: Keerio Azhar Uddin , Nazir Talha , Anwar Tauqir , Zeeshan Majeed Muhammad , Abdulle Yusuf Ali , Jatoi Ghulam Hussain , Gadhi Muswar Ali , Qiu Dewen
Published: August 2020

Plants rely on various physiological and molecular defense mechanisms against biotic stresses such as herbivore insects. Many entomopathogenic fungi synthesize protein molecules that can trigger these plant defenses. This laboratory study characterized the bioactivity of a partially purified protein derived from Beauveria bassiana (ARSEF 2860) against whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), which is an economically important pest of agricultural and horticultural crops worldwide. Different concentrations (i.e., 0.021, 0.042 and 0.063 μM) of fungal protein were bioassayed to determine their sub-lethal effect on the survival percentage and fecundity rate of B. tabaci on tomato (Lycopersicon esculentum) plants. In addition, the putative role of this partially purified B. bassiana protein in the defense mechanisms of plant was assessed through the expression analyses of important genes related to salicylic acid (SA)—and jasmonic acid (JA)—associated pathways using RT-qPCR. Results revealed a significant suppression of the survival percentage and fecundity rate of B. tabaci by the fungal protein. Lowest survival (41%) was recorded for the highest concentration of protein (0.063 μM), whereas mean survival for the other two protein concentrations (0.042 and 0.021 μM) were 62 and 71%, respectively. Likewise, the highest and lowest mean fecundity rates were observed for the control and the highest protein concentration (i.e., 3.3 and 1.8 eggs day−1 female−1, respectively). Furthermore, the exogenous application of B. bassiana-derived protein on tomato plants strongly up-regulated the SA-related genes (PAL, PR1, BGL2 and EDS1) and slightly up-regulated the JA-related genes (AOC, AOS, OPR3 and LOX) as compared to the control plants. These findings demonstrate the putative role of this partially purified B. bassiana protein fraction in inducing systemic resistance in the tomato plants against B. tabaci, suggesting its further purification and characterization to be used as novel biological pest control tool against B. tabaci and other sap-sucking insect pests.
Prevalence of Antibodies to SARS-CoV-2 in Italian Adults and Associated Risk Factors
Authors: Vena Antonio , Berruti Marco , Adessi Andrea , Blumetti Pietro , Brignole Michele , Colognato Renato , Gaggioli Germano , Giacobbe Daniele Roberto , Bracci-Laudiero Luisa , Magnasco Laura , Signori Alessio , Taramasso Lucia , Varelli Marco , Vendola Nicoletta , Ball Lorenzo , Robba Chiara , Battaglini Denise , Brunetti Iole , Pelosi Paolo , Bassetti Matteo
Published: August 2020

We aimed to assess the prevalence of and factors associated with anti- severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) positivity in a large population of adult volunteers from five administrative departments of the Liguria and Lombardia regions. A total of 3609 individuals were included in this analysis. Participants were tested for anti-SARS-CoV-2 antibodies [Immunoglobulin G (IgG) and M (IgM) class antibodies] at three private laboratories (Istituto Diganostico Varelli, Medical Center, and Casa della Salute di Genova). Demographic data, occupational or private exposure to SARS-CoV-2-infected patients, and prior medical history consistent with SARS-CoV-2 infection were collected according to a preplanned analysis. The overall seroprevalence of anti-SARS-CoV-2 antibodies (IgG and/or IgM) was 11.0% [398/3609; confidence interval (CI) 10.0%–12.1%]. Seroprevalence was higher in female inmates than in male inmates (12.5% vs. 9.2%, respectively, p = 0.002), with the highest rate observed among adults aged >55 years (13.2%). A generalized estimating equations model showed that the main risk factors associated with SARS-CoV-2 seroprevalence were the following: an occupational exposure to the virus [Odd ratio (OR) = 2.36; 95% CI 1.59–3.50, p = 0.001], being a long-term care facility resident (OR = 4.53; 95% CI 3.19–6.45, p = 0.001), and reporting previous symptoms of influenza-like illness (OR = 4.86; 95% CI 3.75–6.30, p = 0.001) or loss of sense of smell or taste (OR = 41.00; 95% CI 18.94–88.71, p = 0.001). In conclusion, we found a high prevalence (11.0%) of SARS-CoV-2 infection that is significantly associated with residing in long-term care facilities or occupational exposure to the virus. These findings warrant further investigation into SARS-CoV-2 antibody prevalence among the Italian population.
A Newly Developed Diabetes Risk Index, Based on Lipoprotein Subfractions and Branched Chain Amino Acids, is Associated with Incident Type 2 Diabetes Mellitus in the PREVEND Cohort
Authors: Flores-Guerrero Jose L. , Gruppen Eke. G. , Connelly Margery A. , Shalaurova Irina , Otvos James D. , Garcia Erwin , Bakker Stephan J. L. , Dullaart Robin P. F.
Published: August 2020

Objective: Evaluate the ability of a newly developed diabetes risk score, the Diabetes Risk Index (DRI), to predict incident type 2 diabetes mellitus (T2D) in a large adult population. Methods: The DRI was developed by combining the Lipoprotein Insulin Resistance Index (LP-IR), calculated from 6 lipoprotein subspecies and size parameters, and the branched chain amino acids, valine and leucine, all of which have been shown previously to be associated with future T2D. DRI scores were calculated in a total of 6134 nondiabetic men and women in the Prevention of Renal and Vascular End-Stage Disease (PREVEND) Study. Cox proportional hazards regression was used to evaluate the association of DRI scores with incident T2D. Results: During a median follow-up of 8.5 years, 306 new T2D cases were ascertained. In analyses adjusted for age and sex, there was a significant association between DRI scores and incident T2D with the hazard ratio (HR) for the highest versus lowest quartile being 12.07 (95% confidence interval: 6.97–20.89, p < 0.001). After additional adjustment for body mass index (BMI), family history of T2D, alcohol consumption, diastolic blood pressure, total cholesterol, triglycerides, HDL cholesterol and HOMA-IR, the HR was attenuated but remained significant (HR 3.20 (1.73–5.95), p = 0.001). Similar results were obtained when DRI was analyzed as HR per 1 SD increase (HR 1.37 (1.14–1.65), p < 0.001). The Kaplan–Meier plot demonstrated that patients in the highest quartile of DRI scores presented at higher risk (p-value for log-rank test <0.001). Conclusions: Higher DRI scores are associated with an increased risk of T2D. The association is independent of clinical risk factors for T2D including HOMA-IR, BMI and conventional lipids.
Analysis of the In Situ Steering Motion Characteristics and Sensitivity of Disc-Type Underwater Gliders
Authors: Zhou Han , Yu Pengyao , Jin Xiang , Wang Tianlin
Published: August 2020

A disc-type underwater glider (DTUG) has a highly symmetrical shape and is characterized by omnidirectional characteristics and high maneuverability in small bodies of water. To further explore the disc shape’s advantages and characteristics in steering motion, DTUG motion was simulated by Matlab/Simulink. Based on the structural characteristics of DTUG, the motion control equations were established. The simulation of DTUG’s steering motion is carried out and compared with a previous DTUG (LUNA). The sensitivity analysis and Lyapunov stability analysis were also conducted. The results showed that the in situ steering motion can be realized by controlling the position of the center of gravity (CG) of DTUG without moving vertically, which facilitates rapid adjustment of the yaw angle and flexible movement in small bodies of water. The in situ steering motion was significantly affected by the control parameters. The parameter that had the greatest effect on it can be obtained through sensitivity analysis, which can guide DTUG to better adjust the yaw angle under different conditions. The stability analysis showed that the DTUG can remain stable within the range of the control parameter.
Evaluation of ML-Based Clinical Decision Support Tool to Replace an Existing Tool in an Academic Health System: Lessons Learned
Authors: Woo Myung , Alhanti Brooke , Lusk Sam , Dunston Felicia , Blackwelder Stephen , Lytle Kay S. , Goldstein Benjamin A. , Bedoya Armando
Published: August 2020

There is increasing application of machine learning tools to problems in healthcare, with an ultimate goal to improve patient safety and health outcomes. When applied appropriately, machine learning tools can augment clinical care provided to patients. However, even if a model has impressive performance characteristics, prospectively evaluating and effectively implementing models into clinical care remains difficult. The primary objective of this paper is to recount our experiences and challenges in comparing a novel machine learning-based clinical decision support tool to legacy, non-machine learning tools addressing potential safety events in the hospitals and to summarize the obstacles which prevented evaluation of clinical efficacy of tools prior to widespread institutional use. We collected and compared safety events data, specifically patient falls and pressure injuries, between the standard of care approach and machine learning (ML)-based clinical decision support (CDS). Our assessment was limited to performance of the model rather than the workflow due to challenges in directly comparing both approaches. We did note a modest improvement in falls with ML-based CDS; however, it was not possible to determine that overall improvement was due to model characteristics.
Understanding Landscape Identity in the Context of Rapid Urban Change in China
Authors: Shao Yuhan , Lange Eckart , Thwaites Kevin , Xue Zhenying , Xu Xinyu
Published: August 2020

China is one of the most rapidly urbanising countries in the world. In recent years, it has experienced enormous environmental changes, as well as a loss of landscape identity. This paper aims to explore the concept of landscape identity in the context of the overwhelming urbanisation in China. It develops a conceptual framework on landscape identity from different dimensions. Factors are also identified that drive the urban changes with regard to the landscape identity that develops over time. Taking the city of Yantai, a city in Shandong Province, as an example, it is demonstrated how this conceptual framework can be applied to help in the understanding and protection of landscape identity in China.
Nonexercise Activity Thermogenesis-Induced Energy Shortage Improves Postprandial Lipemia and Fat Oxidation
Authors: Chiu Chih-Hui , Chen Che-Hsiu , Wu Min-Huan , Ding Yin-Fu
Published: August 2020

(1) Background: This study investigated the effect of nonexercise activity thermogenesis on postprandial triglyceride (TG) concentrations; (2) Methods: Ten healthy males completed a sedentary trial (ST) and a physical activity trial (PA) in a random order separated by at least 7 days. After each intervention on day 1, the participants consumed a high-fat test meal on the next day. The blood samples and gas sample were observed in the fasted state and for 4 h after consuming the oral fat tolerance test; (3) Results: The postprandial TG concentrations of total (AUC) (p = 0.008) and incremental area under the curve (IAUC) (p = 0.023) in the plasma of participants in the PA trial were significantly lower than those in the plasma of participants in the ST trial. The postprandial fat oxidation rate AUC of the PA trial was significantly higher than that of the ST trial (p = 0.009); (4) Conclusions: The results of this study indicated that nonexercise energy expenditure decrease the postprandial TG concentration and increase the fat oxidation the next day.
Histological and SEM Assessment of Blood Stasis in Kidney Blood Vessels after Repeated Intra-Arterial Application of Radiographic Contrast Media
Authors: Lamby Philipp , Minkow Alexander , Handt Stefan , Falter Johannes , Schellenberg Eva-Lotte , Graf Stefanie , Hiebl Bernhard , Haerteis Silke , Gemeinhardt Ole , Krüger-Genge Anne , Klosterhalfen Bernd , Jung Ernst-Michael , Franke Ralf-Peter , Momeni Arash , Prantl Lukas , Jung Friedrich
Published: August 2020

Background: After application of iodinated contrast media (CM), a pronounced deterioration of the microcirculation in skin and myocardium was reported. Clinically, the repeated application of CM, especially, led to an increase of the renal resistance index (RRI). With respect to the transiency of the RRI increase, it is reasonable to assume that the deterioration of blood flow could be due to transient blood stasis caused by reversible morphologic cell alterations due to osmotic discrepancies between CM and human blood. Therefore, the hypothesis was investigated whether CM are able to induce in vivo such blood stasis and cell deformations in the renal vasculature of well-hydrated pigs. Methods: The in vivo study was performed as a prospective randomized examination to compare the effects of two different CM in 16 pigs (German Landrace). Pigs were randomized to receive either Iodixanol (n = 8), or Iopromide (n = 8). Each animal received 10 injections separated by 5-min intervals via the suprarenal aorta at a rate of 10 mL/s according to the usual procedure during a cardiac catheter examination. Finally, the kidneys were explanted and processed for histology (H & E staining and fibrin staining according to Weigert) as well as for scanning electron microscopy (SEM) with regards to morphologic correlates explaining the changes in the microcirculation. Results: In each of the predefined four categories of vascular diameters, blood stasis were found, but clearly more often after application of Iopromide than after application of Iodixanol (p < 0.001). In addition, Iopromide induced more blood stasis in all of the examined kidney regions compared to Iodixanol (p = 0.0001). There were no obstructive events in the middle cortex following the application of Iodixanol. Except for the region around a puncture channel of a placed-in catheter probe, no fibrin was detected in Weigert’s fibrin-stained samples, neither around the histologically assessed thrombi nor in vessels with blood stasis. Complementary SEM analyses revealed in a few cases only a slight generation of fibrin and thrombi and deformations, such as echinocyte and “box-like” deformations. Conclusions: According to previous in vitro studies, pathological erythrocyte deformations, such as echinocyte and box-like formation of erythrocytes, were observed also in vivo. In addition, blood stasis and/or thrombi could be detected in histological samples from explanted kidneys from young pigs after repeated in vivo administration of CM. In only a few cases, mural platelet aggregates within minimal fibrin meshes occurred only after the application of Iopromide.
Laser Texturing as a Way of Influencing the Micromechanical and Biological Properties of the Poly(L-Lactide) Surface
Authors: Tomanik Magdalena , Kobielarz Magdalena , Filipiak Jarosław , Szymonowicz Maria , Rusak Agnieszka , Mroczkowska Katarzyna , Antończak Arkadiusz , Pezowicz Celina
Published: August 2020

Laser-based technologies are extensively used for polymer surface patterning and/or texturing. Different micro- and nanostructures can be obtained thanks to a wide range of laser types and beam parameters. Cell behavior on various types of materials is an extensively investigated phenomenon in biomedical applications. Polymer topography such as height, diameter, and spacing of the patterning will cause different cell responses, which can also vary depending on the utilized cell types. Structurization can highly improve the biological performance of the material without any need for chemical modification. The aim of the study was to evaluate the effect of CO2 laser irradiation of poly(L-lactide) (PLLA) thin films on the surface microhardness, roughness, wettability, and cytocompatibility. The conducted testing showed that CO2 laser texturing of PLLA provides the ability to adjust the structural and physical properties of the PLLA surface to the requirements of the cells despite significant changes in the mechanical properties of the laser-treated surface polymer.
Long-Term Behavior and Durability of Alkali-Activated Clay Mortars
Authors: Karozou Aspasia , Konopisi Stavroula , Pavlidou Eleni , Stefanidou Maria
Published: August 2020

The need to increase the durability of clay-based materials, due to their inherent low strength and vulnerability in contact with water, led researchers to examine different options. In this paper, clay mortars were produced using four different activating solutions. Alkali hydroxides, alkali carbonates, and alkali silicates activating solutions were used. Interest is given to long term properties while their behavior to wetting–drying and freeze–thaw cycles is recorded. In total, the results of the experiments indicated the positive effect of the potassium metasilicate on mechanical characteristics presenting, however, low performance at wetting–drying. The combination of sodium metasilicate with sodium hydroxide solution has also presented a positive effect on both mechanical and physical properties. In contrast, sodium carbonate acted better in enhancing physical properties and granting water-resistant abilities. Moreover, the performance of the specimens mixed with water–glass addition presented excellent volume stability and low mass loss in durability tests.
Chitosan Films Incorporated with Exopolysaccharides from Deep Seawater Alteromonas sp.
Authors: Zarandona Iratxe , Estupiñán Mónica , Pérez Carla , Alonso-Sáez Laura , Guerrero Pedro , de la Caba Koro
Published: August 2020

Two Alteromonas sp. strains isolated from deep seawater were grown to promote the production of exopolysaccharides (EPS, E611 and E805), which were incorporated into chitosan solutions to develop films. The combination of the major marine polysaccharides (chitosan and the isolated bacterial EPS) resulted in the formation of homogenous, transparent, colorless films, suggesting good compatibility between the two components of the film-forming formulation. With regards to optical properties, the films showed low values of gloss, in the range of 5–10 GU, indicating the formation of non-glossy and rough surfaces. In addition to the film surface, both showed hydrophobic character, with water contact angles higher than 100 º, regardless of EPS addition. Among the two EPS under analysis, chitosan films with E805 showed better mechanical performance, leading to resistant, flexible, easy to handle films.
Plasticized PVC Membrane Modified Electrodes: Voltammetry of Highly Hydrophobic Compounds
Authors: Lindner Ernő , Guzinski Marcin , Pendley Bradford , Chaum Edward
Published: August 2020

In the last 50 years, plasticized polyvinyl chloride (PVC) membranes have gained unique importance in chemical sensor development. Originally, these membranes separated two solutions in conventional ion-selective electrodes. Later, the same membranes were applied over a variety of supporting electrodes and used in both potentiometric and voltammetric measurements of ions and electrically charged molecules. The focus of this paper is to demonstrate the utility of the plasticized PVC membrane modified working electrode for the voltammetric measurement of highly lipophilic molecules. The plasticized PVC membrane prevents electrode fouling, extends the detection limit of the voltammetric methods to sub-micromolar concentrations, and minimizes interference by electrochemically active hydrophilic analytes.
Titanium Scaffolds by Direct Ink Writing: Fabrication and Functionalization to Guide Osteoblast Behavior
Authors: Vidal Elia , Torres Diego , Guillem-Marti Jordi , Scionti Giuseppe , Manero José María , Ginebra Maria-Pau , Rodríguez Daniel , Rupérez Elisa
Published: August 2020

Titanium (Ti) and Ti alloys have been used for decades for bone prostheses due to its mechanical reliability and good biocompatibility. However, the high stiffness of Ti implants and the lack of bioactivity are pending issues that should be improved to minimize implant failure. The stress shielding effect, a result of the stiffness mismatch between titanium and bone, can be reduced by introducing a tailored structural porosity in the implant. In this work, porous titanium structures were produced by direct ink writing (DIW), using a new Ti ink formulation containing a thermosensitive hydrogel. A thermal treatment was optimized to ensure the complete elimination of the binder before the sintering process, in order to avoid contamination of the titanium structures. The samples were sintered in argon atmosphere at 1200 °C, 1300 °C or 1400 °C, resulting in total porosities ranging between 72.3% and 77.7%. A correlation was found between the total porosity and the elastic modulus of the scaffolds. The stiffness and yield strength were similar to those of cancellous bone. The functionalization of the scaffold surface with a cell adhesion fibronectin recombinant fragment resulted in enhanced adhesion and spreading of osteoblastic-like cells, together with increased alkaline phosphatase expression and mineralization.
Strain Rate Dependence of Hardness for PE and SME TiNi Alloys
Authors: Shen Lingyan , Hui Mengmeng , Liu Yonggui
Published: August 2020

In this paper, the strain rate dependence of hardening behavior of polycrystalline pseudoelastic (PE) and shape memory effect (SME) TiNi alloy under impact loading was investigated by experiments. Measurements of stress–strain curves, hardening modulus, hysteresis loop area, and temperature variation are synchronized using in situ infrared detector system at the strain rate range from 300/s to 2000/s. It is shown that with the strain rate increasing, for PE specimens, strain rate hardening is observed, while SME specimens perform a strong nonlinear strain hardening. The results of synchronous temperature measurement show that in stress-temperature space, for PE samples, the dynamic transformation path is strain rate independent, but for the SME samples, the opposite is true. Thermal-mechanical coupling does not seem to explain this difference, and hardening from microstructure variation should be considered for such difference.
Improvement the Plasticity via Dual-Amorphous and Nanocrystals Synergistically in a Zr-Cu-Al-Nb Bulk Metallic Glass Composite
Authors: Wang Tuo , Yang Xiaohui , Li Qiang , Chang Chuntao
Published: August 2020

In this work, a small amount of Nb has been added in a Zr52Cu42.5Al5.5 bulk metallic glass, and a Zr52Cu42Al5.5Nb0.5 bulk metallic glass composite with dual-amorphous and nanocrystal structures has been developed for the first time. This in situ formed bulk metallic glass composite has a larger room compressive plasticity of above 13% than that of the Zr52Cu42.5Al5.5 bulk metallic glass. The excellent plasticity of the bulk metallic glass composite is attributed to the phase-separated matrix with micro-nanocrystal and the nanocrystallization during the deforming process. This work may give a new sight into design bulk metallic glass composites and the underlying mechanism for deformation.
Numerical Analysis of Effect of Initial Bubble Size on Captured Bubble Distribution in Steel Continuous Casting Using Euler-Lagrange Approach Considering Bubble Coalescence and Breakup
Authors: Yang Weidong , Luo Zhiguo , Zhao Nannan , Zou Zongshu
Published: August 2020

A mathematic model considering the bubble coalescence and breakup using the Euler-Lagrange approach has been developed to study the effect of the initial bubble size on the distribution of bubbles captured by the solidification shell. A hard sphere model was applied for dealing with the bubble collision. Advanced bubble coalescence and breakup models suitable for the continuous casting system and an advanced bubble captured criteria have been identified established with the help of user-defined functions of FLUENT. The predictions of bubble behavior and captured bubble distribution agree with the water model and plant measurements well respectively. The results show that the number of small bubbles captured by solidification shell is much higher than that of large bubbles. What is more, the number of captured bubbles at the sidewalls decreases with the distance from the meniscus. For the case of large gas flow rate (gas flow fraction of 8.2%), the initial size of bubbles has little effect on bubble captured distribution under various casting speeds. When the gas flow rate is small (gas flow fraction of 4.1%), the number density of captured bubbles increases as the initial bubble size increases, and the effect of initial bubbles size on captured bubble number density is amplified when the casting speed decreases. The average captured bubble diameter is about 0.12–0.14 mm. Additionally, for all cases, the initial bubble size hardly affects the average size of captured bubbles.
Bridging Targeted and Untargeted Mass Spectrometry-Based Metabolomics via Hybrid Approaches
Authors: Chen Li , Zhong Fanyi , Zhu Jiangjiang
Published: August 2020

This mini-review aims to discuss the development and applications of mass spectrometry (MS)-based hybrid approaches in metabolomics. Several recently developed hybrid approaches are introduced. Then, the overall workflow, frequently used instruments, data handling strategies, and applications are compared and their pros and cons are summarized. Overall, the improved repeatability and quantitative capability in large-scale MS-based metabolomics studies are demonstrated, in comparison to either targeted or untargeted metabolomics approaches alone. In summary, we expect this review to serve as a first attempt to highlight the development and applications of emerging hybrid approaches in metabolomics, and we believe that hybrid metabolomics approaches could have great potential in many future studies.
Camphor-Based CVD Bilayer Graphene/Si Heterostructures for Self-Powered and Broadband Photodetection
Authors: Tsai Dung-Sheng , Chiang Ping-Yu , Tsai Meng-Lin , Tu Wei-Chen , Chen Chi , Chen Shih-Lun , Chiu Ching-Hsueh , Li Chen-Yu , Uen Wu-Yih
Published: August 2020

This work demonstrates a self-powered and broadband photodetector using a heterojunction formed by camphor-based chemical vaper deposition (CVD) bilayer graphene on p-Si substrates. Here, graphene/p-Si heterostructures and graphene layers serve as ultra-shallow junctions for UV absorption and zero bandgap junction materials (95% coverage bilayer and high-uniformity graphene were successfully obtained by camphor-based CVD processes. Furthermore, the carrier mobility of the camphor-based CVD bilayer graphene at room temperature is 1.8 × 103 cm2/V·s. Due to the incorporation of camphor-based CVD graphene, the graphene/p-Si Schottky junctions show a good rectification property (rectification ratio of ~110 at ± 2 V) and good performance as a self-powered (under zero bias) photodetector from UV to LWNIR. The photocurrent to dark current ratio (PDCR) value is up to 230 at 0 V under white light illumination, and the detectivity (D*) is 8 × 1012 cmHz1/2/W at 560 nm. Furthermore, the photodetector (PD) response/decay time (i.e., rise/fall time) is ~118/120 μs. These results support the camphor-based CVD bilayer graphene/Si Schottky PDs for use in self-powered and ultra-broadband light detection in the future.
Flotation Behavior of Malachite Using Hydrophobic Talc Nanoparticles as Collectors
Authors: Choi Junhyun , Seo Joobeom , Kim Sang Bae , Kim Wantae
Published: August 2020

In this study, the flotation behavior of malachite was investigated using hydrophobic talc nanoparticles (TNs) as collectors. To improve the floatability of TN-deposited malachite, various experimental parameters were systematically investigated. We found that the floatability sharply increased as the size of the TNs decreased. The floatability of malachite was enhanced in the presence of smaller TNs, since higher amounts of smaller TNs were deposited on the surface of the malachite, thus rendering the surface more hydrophobic. Moreover, the floatability of the TN-deposited malachite increased as the pH decreased, likely due to the more favorable interaction between TNs and malachite by means of electrostatic attraction. Furthermore, the floatability became more enhanced as the TN concentration increased, likely associated with increases in the amount of TNs deposited on the surface of the malachite, thus enhancing the floatability by altering the hydrophobicity of the surface. Our findings suggest that the application of natural hydrophobic TNs as collectors in malachite flotation should be introduced as a new concept.