bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the Applicability of Ichthyoplanktonic Indices in Better Understanding the Dynamics of the Northern Stock of the Population of Atlantic Hake Merluccius merluccius (L.)
Authors: Alvarez Paula , Garcia Dorleta , Cotano Unai
Published: January 2023

Abundance indices are essential data for the application of stock assessment models to obtain fish abundance estimates. Abundance indices have usually been derived from fishery-dependent data, yet the increase in fisheries-independent surveys is now offering new opportunities for these calculations. In this study, we explored the usefulness of ichthyoplankton indices derived from scientific surveys in estimating spawning biomass. In addition, we also investigated whether the strength of the year–class of the commercial cohort of Atlantic hake, as a determinant, could be defined at an early life stage. We used samples collected during the triennial mackerel and horse mackerel egg surveys (MEGS), which cover the hake spawning area in the Bay of Biscay. The biomass indices were determined as the abundance of eggs in the early development stage (stage 1) when transformed into egg production (EP) from 1995 to 2019 in the months of March and April—which is considered a period of high spawning activity for hake in this area. Additionally, we built a metric for larval abundance and converted larval length into age. This was in addition to constructing a pre-recruit year-class index (YCI) while using the EVHOE bottom trawl abundance database for hake for the period of 1997 to 2016. The results of regression analysis of egg production and spawning stock biomass indicate that both parameters are significantly correlated (r = 0.76). By connecting the abundance of eggs and larvae in the adjoining stages, we are able to identify two periods of high mortality associated with the transition from “yolk-sac-first” to “feeding larvae” and “late larvae-YCI10”, but we were unable to discover when the strength of the recruitment year–class is determined. As such, it appears that for the northern stock of hake, recruitment is established in the late juvenile stages.
Analyzing Asymmetric Volatility and Multifractal Behavior in Cryptocurrencies Using Capital Asset Pricing Model Filter
Authors: Lee Minhyuk , Cho Younghwan , Ock Seung Eun , Song Jae Wook
Published: January 2023

This research analyzes asymmetric volatility and multifractality in four representative cryptocurrencies using index-based asymmetric multifractal detrended fluctuation analysis. We suggest investigating an idiosyncratic risk premium, which can be obtained by removing the market influence in the cryptocurrency return series. We call the process a capital asset pricing model filter. The analyses on the original return series showed no significant sign of asymmetric volatility. However, the filter revealed a distinct asymmetric volatility, distinguishing the uptrend and downtrend fluctuations. Furthermore, the analyses on the idiosyncratic risk premium detected some cases of asymmetry in the degree and source of multifractality, whereas that on the original return series failed to detect the asymmetry. In conclusion, in a highly volatile market, the capital asset pricing model filter can improve an investigation of the asymmetric multifractality in cryptocurrencies.
Design of Injectable Bioartificial Hydrogels by Green Chemistry for Mini-Invasive Applications in the Biomedical or Aesthetic Medicine Fields
Authors: Laurano Rossella , Boffito Monica , Cassino Claudio , Liberti Francesco , Ciardelli Gianluca , Chiono Valeria
Published: January 2023

Bioartificial hydrogels are hydrophilic systems extensively studied for regenerative medicine due to the synergic combination of features of synthetic and natural polymers. Injectability is another crucial property for hydrogel mini-invasive administration. This work aimed at engineering injectable bioartificial in situ cross-linkable hydrogels by implementing green and eco-friendly approaches. Specifically, the versatile poly(ether urethane) (PEU) chemistry was exploited for the development of an amphiphilic PEU, while hyaluronic acid was selected as natural component. Both polymers were functionalized to expose thiol and catechol groups through green water-based carbodiimide-mediated grafting reactions. Functionalization was optimized to maximize grafting yield while preserving group functionality. Then, polymer miscibility was studied at the macro-, micro-, and nano-scale, suggesting the formation of hydrogen bonds among polymeric chains. All hydrogels could be injected through G21 and G18 needles in a wide temperature range (4–25 °C) and underwent sol-to-gel transition at 37 °C. The addition of an oxidizing agent to polymer solutions did not improve the gelation kinetics, while it negatively affected hydrogel stability in an aqueous environment, suggesting the occurrence of oxidation-triggered polymer degradation. In the future, the bioartificial hydrogels developed herein could find application in the biomedical and aesthetic medicine fields as injectable formulations for therapeutic agent delivery.
Development and Evaluation of Cellulose Derivative and Pectin Based Swellable pH Responsive Hydrogel Network for Controlled Delivery of Cytarabine
Authors: Batool Nighat , Sarfraz Rai Muhammad , Mahmood Asif , Rehman Umaira , Zaman Muhammad , Akbar Shehla , Almasri Diena M. , Gad Heba A.
Published: January 2023

In the present study, pH-sensitive, biodegradable, and biocompatible Na-CMC/pectin poly(methacrylic acid) hydrogels were synthesized using an aqueous free radical polymerization technique and encapsulated by cytarabine (anti-cancer drug). The aim of the project was to sustain the plasma profile of cytarabine through oral administration. Sodium carboxymethyl cellulose (Na-CMC) and pectin were cross-linked chemically with methacrylic acid (MAA) as a monomer, using methylene bisacrylamide (MBA) as cross-linker and ammonium per sulfate (APS) as an initiator. Prepared hydrogel formulations were characterized for their texture, morphology, cytarabine loading efficiency, compositional and structural properties, thermal nature, stability, swelling response, drug release profile (pH 1.2 and pH 7.4), and in-vivo pharmacokinetic evaluation. Cytarabine-loaded hydrogels were also evaluated for their safety profile by carrying out toxicity studies in rabbits. Results demonstrated efficient encapsulation of cytarabine into the prepared network with loading ranging from 48.5–82.3%. The highest swelling ratio of 39.38 and maximum drug release of 83.29–85.27% were observed at pH 7.4, highlighting the pH responsiveness of the grafted system. Furthermore, cytarabine maximum release was noticed over 24 h, ensuring a sustained release response for all formulations. Histopathological studies and hemolytic profiles confirmed that the prepared hydrogel system was safe, biocompatible, and non-irritant, showing no symptoms of any toxicities and degeneration in organs. Moreover, pharmacokinetic estimation of the cytarabine-loaded hydrogel showed a remarkable increase in the plasma half-life from 4.44 h to 9.24 h and AUC from 22.06 μg/mL.h to 56.94 μg/mL.h. This study revealed that the prepared hydrogel carrier system has excellent abilities in delivering the therapeutic moieties in a controlled manner.
Kaniwa (Chenopodium pallidicaule)’s Nutritional Composition and Its Applicability as an Elder-Friendly Food with Gelling Agents
Authors: Kim Dah-Sol , Iida Fumiko
Published: January 2023

(1) Background: This study attempted to develop an elder-friendly food suitable to the Korean Industrial Standard (KS) after identifying the nutritional characteristics of Kaniwa; (2) Methods: The nutrient composition and physiological activity of Kaniwa were analyzed, and the concentration of the gelling agent (guar gum, locust bean gum, and xanthan gum) to be added to Kaniwa mousse was derived through regression analysis to suit KS hardness level 1 to 3; (3) Results: It was found that Kaniwa not only had a good fatty acid composition but also had good antioxidant and anti-diabetic properties. Moreover, it was found that in order to have the hardness to chew Kaniwa mousse with the tongue, it was necessary to add less than 1.97% guar gum, 4.03% locust bean gum, and 8.59% xanthan gum. In order to have a hardness that can be chewed with the gum, it was found that 2.17~4.97% guar gum, 4.45~10.28% locust bean gum, and 9.48~21.96% xanthan gum should be added; (4) Conclusions: As the aging rate and life expectancy increase, support for developmental research related to the elder-friendly industry should be continuously expanded in preparation for the upcoming super-aging society.
Women Physicians and Their Careers: Athens—1900–1950: A Contribution to Understanding Women’s History
Authors: Bournova Eugenia , Dimitropoulou Myrto
Published: January 2023

This article combines history of the family with women’s and gender history and the history of women’s education; it is based on an extensive range of archives and aims at highlighting the attitude of society and families towards women who wanted to attend University studies in the beginning of the 20th century. The matter of women’s university education is directly related to the emergence of the feminist movement in Greece. The strong preference of female university students for the exact sciences at that time was justified by contemporary scholars as a choice reflecting women’s nature. This article highlights the role played by family and social class background. To this effect, the life course of three ‘heroines’ is followed from their initial desire to undertake further studies to their participation in the social and cultural life of the capital of Greece, as a contribution to current literature on gender studies. Despite the limited number of cases discussed, we strongly believe that these women’s upbringing enhances our understanding of women’s scientific pursuits and their place in Athenian elite families.
Selection Progress for Resistance to Fusarium Basal Rot in Short-Day Onions Using Artificial Inoculation Mature Bulb Screening
Authors: Sharma Suman , Cramer Christopher S.
Published: January 2023

Fusarium basal rot (FBR), caused by a soil-borne fungus, Fusarium oxysporum f. sp. cepae (FOC), is a major disease hindering onion production worldwide. This study was conducted to evaluate the initial and the most advanced selected populations of seven open-pollinated short-day onion cultivars for FBR susceptibility, along with two check cultivars using the conidial inoculation of mature bulbs for two consecutive years. The artificial inoculation of mature bulbs was carried out by applying a virulent FOC isolate ‘CSC 515’ at a final concentration of 3.0 × 104 spores mL−1 to the transversely cut basal plates of onion bulbs. The basal plates of 20 arbitrarily chosen bulbs per plot were recut after 20 days of incubation and then were rated for FBR severity using a rating scale of 1 (no disease) to 9 (≥70% of the basal plate is infected). The bulbs with a rating of 1 were saved and then bulked to form the seeds for the next generation. The selected populations exhibited a variable response for FBR severity when evaluated over two years, with an improvement in the most advanced selections observed for a majority of the cultivars. For example, the advanced selections of ‘NuMex Sweetpak’ exceeded the partially resistant check ‘Serrana’ in their levels of resistance when both were evaluated in the second year. A conidial inoculation can be effective in the development of FBR-resistant cultivars. In addition, this inoculation method can accelerate breeding efforts by determining the genetic mechanism(s) responsible for FBR resistance, locating quantitative trait loci, and facilitating marker-assisted selection.
Propagation Characteristics of the Rare Endemic Rhododenderon micranthum Turcz.
Authors: Oh Sang Im , Park Eun Hye , Koh Hong Min , Gil Min , Yoon Mi Jeong , Bae Jun Kyu , Ko Chung Ho
Published: January 2023

This study was conducted to examine the effect of plant growth regulators on the rooting and growth of Rhododendron micranthum Turcz., a rare plant in South Korea. Indole-3-butyric acid (IBA) 150, 250, 350 mg·L−1 and α-naphthaleneacetic acid (NAA) 50, 150, 250 mg·L−1 treatment groups were established and cutting was performed in a commercial growth medium in March 2021 with 3 replications of 10 plants per group. After 70 days, the longest initial root growth was observed under the NAA 150 and 250 mg·L−1 treatments. Moreover, the number of roots and percentage of rooting were slightly higher in NAA 150 and 250 mg·L−1, indicating that these treatments were more effective in promoting plant growth. The above-ground part of plants treated with NAA 150 and 250 mg·L−1 had a taller plant height, longer and wider leaves, and a greater number of leaves compared to other groups. Accordingly, the survival rate of plants treated with NAA 150 and 250 mg·L−1 was higher than 80%, suggesting well-established roots and excellent above-ground growth. Therefore, treatment with growth regulator NAA at 150 and 250 mg·L−1 is considered beneficial for the cutting of R. micranthum Turcz.
Positive Impacts of a Four-Week Neuro-Meditation Program on Cognitive Function in Post-Acute Sequelae of COVID-19 Patients: A Randomized Controlled Trial
Authors: Hausswirth Christophe , Schmit Cyril , Rougier Yann , Coste Alexandre
Published: January 2023

Study objective: Long COVID patients can experience high levels of impairment in their cognitive function and mental health. Using a parallel randomized control trial, we evaluated the effectiveness of a neuro-meditation program to reduce cognitive impairment in patients with long COVID. Methods: A total of 34 patients with long COVID were randomized to an intervention group (G-Int; n = 17) or a control group (G-Con; n = 17) and 15 healthy participants were constitutive of a normative group (G-Nor). The intervention consisted of ten 30-min sessions of Rebalance® over a period of five weeks. Each session included sound therapy and coach-guided meditation associated with light stimulations (i.e., chromotherapy). Primary outcomes were performance on five computerized cognitive tasks (choice response time, pattern comparison, Simon, pursuit rotor task, and Corsi block-tapping task), mental and physical fatigue (Chalder fatigue scale), perceived stress (perceived stress scale) and mood (profiles of mood states). Secondary outcomes were anxiety and depressive symptoms (hospital anxiety and depression scale), muscular pain, joint pain, and headaches using visual analog scales (VAS) as well as sleep quality (Spiegel sleep questionnaire). Assessments were conducted at baseline and at 1–2 and 7–8 days of follow-up. Results: Compared to healthy subjects, long COVID patients showed significant differences at baseline on all the self-report questionnaires, and a Rebalance® program improved all the subjective reports, as well as cognitive performances, especially on reaction time-based tasks. In particular, only the G-Int group revealed shortened reaction times in the choice reaction time (RTbaseline = 593 ± 121 ms vs. RTpost2 = 521 ± 86 ms, p < 0.001), Simon (RTbaseline = 539 ± 123 ms vs. RTpost2 = 494 ± 134 ms, p < 0.01), and pattern comparison tasks (RTbaseline = 1244 ± 315 ms vs. RTpost2 = 1079 ± 213 ms, p < 0.001). Conclusions) Initial evidence suggests that neuro-meditation reduces cognitive impairment and improves physical and mental fatigue, muscle and joint pain, symptoms of depression and anxiety, mood disturbances as well as sleep quality. The Rebalance® program hence constitutes a promising non-pharmacological intervention for the treatment of long-term psychological/cognitive outcomes of COVID-19.
The Safety and Efficacy of 1-Monoeicosapentaenoin Isolated from the Trebouxiophyceae Micractinium on Anti-Wrinkle: A Split-Face Randomized, Double-Blind Placebo-Controlled Clinical Study
Authors: Kim Ki Mo , Ji Kon-Young , Choi Yoon Jung , Heo Jong Beom , Youn Ui Joung , Kim Sanghee , Shim Ki-Shuk , Lee Joo Young , Kim Tae Soo , Seo Young Kyoung , Song Gyu-Yong , Chae Sungwook
Published: January 2023

The skin aging process is governed by intrinsic and extrinsic factors causing skin wrinkles, sagging, and loosening. The 1-monoeicosapentaenoin (1-MEST) is a component isolated from Micractinium, a genus of microalgae (Chlorophyta, Trebouxiophyceae). However, the anti-wrinkle effects of 1-MEST are not yet known. This study aimed to evaluate the anti-wrinkle effects of 1-MEST in vitro and in clinical trials. The cytotoxicity of 1-MEST was investigated in vitro using the MTS assay in human epidermal keratinocytes (HEKs). Expression of matrix metalloproteinase (MMP)-1 and MMP-9 was determined by ELISA in HEKs irradiated with UVB after treatment with 1-MEST. A split-face randomized, double-blind, placebo-controlled study was conducted to evaluate the safety and efficacy of 1-MEST. The study evaluated wrinkle parameters and visual assessment, self-efficacy and usability questionnaires, and adverse events. The study showed that the 1-MEST was not cytotoxic in HEKs, suppressed MMP-1 secretion and MMP-9 protein expression in HEKs irradiated with UVB. The wrinkle parameters and mean visual assessment score were significantly decreased in the test group after 12 weeks and differed from the control group. There were no significant differences in efficacy and usability. Adverse effects were also not observed. The 1-MEST showed anti-wrinkle properties to slow down or prevent skin aging.
Partogram of Grandmultiparous Parturients: A Multicenter Cohort Study
Authors: Guedalia Joshua , Lipschuetz Michal , Walfisch Asnat , Cohen Sarah , Sheiner Eyal , Samson Abraham , Rosenbloom Joshua , Kabiri Doron , Hochler Hila
Published: January 2023

Sparse and conflicting data exist regarding the normal partogram of grand-multiparous (GMP, defined as parity of 6+) parturients. Customized partograms may potentially lower cesarean delivery rates for protraction disorders in this population. In this study, we aim to construct a normal labor curve of GMP women and compare it to the multiparous (MP, defined as parity of 2–5) partogram. We conducted a multicenter retrospective cohort analysis of deliveries between the years 2003 and 2019. Eligible parturients were the trials of labor of singletons ≥37 + 0 weeks in cephalic presentation with ≥2 documented cervical examinations during labor. Exclusion criteria were elective cesarean delivery without a trial of labor, preterm labor, major fetal anomalies, and fetal demise. GMP comprised the study group while the MP counterparts were the control group. A total of 78,292 deliveries met the inclusion criteria, comprising 10,532 GMP and 67,760 MP parturients. Our data revealed that during the first stage of labor, cervical dilation progressed at similar rates in MPs and GMPs, while head descent was a few minutes faster in GMPs compared to MPs, regardless of epidural anesthesia. The second stage of labor was faster in GMPs compared to MPs; the 95th percentile of the second stage duration of GMPs (48 min duration) was 43 min less than that of MPs (91 min duration). These findings remained similar among deliveries with and without epidural analgesia or labor induction. We conclude that GMPs’ and MPs’ cervical dilation progression in the active phase of labor was similar, and the second stage of labor was shorter in GMPs, regardless of epidural use. Thus, GMPs’ uterus function during labor corresponds, and possibly surpasses, that of MPs. These findings indicate that health providers can use the standard partogram of the active phase of labor when caring for GMP parturients.
DR5 Up-Regulation Induced by Dichloroacetate Sensitizes Tumor Cells to Lipid Nanoparticles Decorated with TRAIL
Authors: Marco-Brualla Joaquín , de Miguel Diego , Martínez-Lostao Luis , Anel Alberto
Published: January 2023

Cancer resistance to treatments is a challenge that researchers constantly seek to overcome. For instance, TNF-related apoptosis-inducing ligand (TRAIL) is a potential good prospect as an anti-cancer therapy, as it attacks tumor cells but not normal cells. However, treatments based in soluble TRAIL provided incomplete clinical results and diverse formulations have been developed to improve its bioactivity. In previous works, we generated a new TRAIL formulation based in its attachment to the surface of unilamellar nanoliposomes (LUV-TRAIL). This formulation greatly increased apoptosis in a wide selection of tumor cell types, albeit a few of them remained resistant. On the other hand, it has been described that a metabolic shift in cancer cells can also alter its sensitivity to other treatments. In this work, we sought to increase the sensitivity of several tumor cell types resistant to LUV-TRAIL by previous exposure to the metabolic drug dichloroacetate (DCA), which forces oxidative phosphorylation. Results showed that DCA + LUV-TRAIL had a synergistic effect on both lung adenocarcinoma A549, colorectal HT29, and breast cancer MCF7 cells. Despite DCA inducing intracellular changes in a cell-type specific way, the increase in cell death by apoptosis was clearly correlated with an increase in death receptor 5 (DR5) surface expression in all cell lines. Therefore, DCA-induced metabolic shift emerges as a suitable option to overcome TRAIL resistance in cancer cells.
Application of the Active-Fluidics System in Phacoemulsification: A Review
Authors: Luo Yu , Xu Guangcan , Li Hongyu , Ma Tianju , Ye Zi , Li Zhaohui
Published: January 2023

The fluidics system is an indispensable and primary component of phacoemulsification. Both the gravity-fluidics system and active-fluidics system are commonly used in practice. The irrigation pressure of the gravity-fluidics system is determined by the bottle height, which is relatively constant, while the active-fluidics system is paired with a cassette that contains pressure sensors to monitor intraocular pressure changes. The active-fluidics system allows surgeons to preset a target intraocular pressure value, and it replenishes the fluids proactively; thus, the intraocular pressure is consistently maintained near the target value. Under such circumstances, stable intraocular pressure and anterior chamber volume values could be acquired. Research on surgical safety, efficiency and results have reported several strengths of the active-fluidics system. It is also advantageous in some complicated cataract surgeries. However, the system is not widely used at present, mainly due to its low penetration rate and high equipment cost. Some of its updates such as the new Active Sentry handpiece showed potential superiority in laboratory studies recently, but there is still further research to be conducted. This article gives an overview of the mechanism and performance of the active-fluidics system, and it is expected to provide clues for future research.
Sustainable Composites from Waste Sulfur, Terpenoids, and Pozzolan Cements
Authors: Tisdale Katelyn A. , Maladeniya Charini P. , Lopez Claudia V. , Tennyson Andrew G. , Smith Rhett C.
Published: January 2023

Sulfur cements have drawn significant attention as binders because sulfur is a byproduct of fossil fuel refining. Sulfur cements that can be formed by the vulcanization of elemental sulfur and plant-derived olefins such as terpenoids are particularly promising from a sustainability standpoint. A range of terpenoid–sulfur cements have shown compressional and flexural properties exceeding those of some commercial structural mineral cements. Pozzolans such as fly ash (FA), silica fume (SF), and ground granulated blast furnace slag (GGBFS) and abundant clay resources such as metakaolin (MK) are attractive fines for addition to binders. Herein, we report 10 composites prepared by a combination of sulfur, terpenoids (geraniol or citronellol), and these pozzolans. This study reveals the extent to which the addition of the pozzolan fines to the sulfur–terpenoid cements influences their mechanical properties and chemical resistance. The sulfur–terpenoid composites CitS and GerS were prepared by the reaction of 90 wt% sulfur and 10 wt% citronellol or geraniol oil, respectively. The density of the composites fell within the range of 1800–1900 kg/m3 and after 24 h submersion in water at room temperature, none of the materials absorbed more than 0.7 wt% water. The compressional strength of the as-prepared materials ranged from 9.1–23.2 MPa, and the percentage of compressional strength retained after acid challenge (submersion in 0.1 M H2SO4 for 24 h) ranged from 80–100%. Incorporating pozzolan fines into the already strong CitS (18.8 MPa) had negligible effects on its compressional strength within the statistical error of the measurement. CitS-SF and CitS-MK had slightly higher compressive strengths of 20.4 MPa and 23.2 MPa, respectively. CitS-GGBFS and CitS-FA resulted in slightly lower compressive strengths of 17.0 MPa and 15.8 MPa, respectively. In contrast, the compressional strength of initially softer GerS (11.7 MPa) benefited greatly after incorporating hard mineral fines. All GerS derivatives had higher compressive strengths than GerS, with GerS-MK having the highest compressive strength of 19.8 MPa. The compressional strengths of several of the composites compare favorably to those required by traditional mineral cements for residential building foundations (17 MPa), whereas such mineral products disintegrate upon similar acid challenge.
Study of the Effects of Alkali Treatment and Fiber Orientation on Mechanical Properties of Enset/Sisal Polymer Hybrid Composite
Authors: Bekele Abera E. , Lemu Hirpa G. , Jiru Moera G.
Published: January 2023

In the manufacturing process of innovative fiber-based composite materials, natural fibers are among the most commonly employed reinforcements. In this study, Enset/Sisal (E/S) fiber with a polyester matrix was used to develop the hybrid composites. Hand layup methods were employed for the sample preparation from untreated, 5%, and 10% alkali-treated unidirectional and woven fiber orientations having 50:50 volume ratios. The mechanical properties and water absorption of natural fiber hybrid composites were influenced by fiber treatment and orientation. In the present investigation, the result shows that treated and woven fiber orientation hybrid composites exhibit better mechanical properties than untreated and unidirectional E/S hybrid composites. The 5% NaOH-treated samples have higher tensile and flexural strength properties than the untreated and 10% alkali-treated composites, while the 5% NaOH-treated fiber composites have lower water absorption properties. The tensile and flexural strengths and impacts of 5% NaOH-treated composites were improved by 5.21%, 9.25%, and 5.98%, respectively, over untreated E/S hybrid composites. The morphological properties of the fracture surface of the composite were observed using scanning electron microscopy (SEM).
Numerical Optimization of a Nonlinear Nonideal Piezoelectric Energy Harvester Using Deep Learning
Authors: Hegendörfer Andreas , Steinmann Paul , Mergheim Julia
Published: January 2023

This contribution addresses the numerical optimization of the harvested energy of a mechanically and electrically nonlinear and nonideal piezoelectric energy harvester (PEH) under triangular shock-like excitation, taking into account a nonlinear stress constraint. In the optimization problem, a bimorph electromechanical structure equipped with the Greinacher circuit or the standard circuit is considered and different electrical and mechanical design variables are introduced. Using a very accurate coupled finite element-electronic circuit simulator method, deep neural network (DNN) training data are generated, allowing for a computationally efficient evaluation of the objective function. Subsequently, a genetic algorithm using the DNNs is applied to find the electrical and mechanical design variables that optimize the harvested energy. It is found that the maximum harvested energy is obtained at the maximum possible mechanical stresses and that the optimum storage capacitor for the Greinacher circuit is much smaller than that for the standard circuit, while the total harvested energy by both configurations is similar.
Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling
Authors: Bai Qian , Wang Xin , Yan Hongxiang , Wen Lishi , Zhou Ziyi , Ye Yating , Jing Yutong , Niu Yali , Wang Liang , Zhang Zifeng , Su Jingbo , Chang Tianfang , Dou Guorui , Wang Yusheng , Sun Jiaxing
Published: January 2023

Pathological retinal neovascularization (RNV) is the main character of ischemic ocular diseases, which causes severe visual impairments. Though retinal microglia are well acknowledged to play important roles in both physiological and pathological angiogenesis, the molecular mechanisms by which microglia communicates with endothelial cells (EC) remain unknown. In this study, using single-cell RNA sequencing, we revealed that the pro-inflammatory secreted protein Spp1 was the most upregulated gene in microglia in the mouse model of oxygen-induced retinopathy (OIR). Bioinformatic analysis showed that the expression of Spp1 in microglia was respectively regulated via nuclear factor-kappa B (NF-κB) and hypoxia-inducible factor 1α (HIF-1α) pathways, which was further confirmed through in vitro assays using BV2 microglia cell line. To mimic microglia-EC communication, the bEnd.3 endothelial cell line was cultured with conditional medium (CM) from BV2. We found that adding recombinant Spp1 to bEnd.3 as well as treating with hypoxic BV2 CM significantly enhanced EC proliferation and migration, while Spp1 neutralizing blocked those CM-induced effects. Moreover, RNA sequencing of BV2 CM-treated bEnd.3 revealed a significant downregulation of Kit, one of the type III tyrosine kinase receptors that plays a critical role in cell growth and activation. We further revealed that Spp1 increased phosphorylation and expression level of Akt/mTOR signaling cascade, which might account for its pro-angiogenic effects. Finally, we showed that intravitreal injection of Spp1 neutralizing antibody attenuated pathological RNV and improved visual function. Taken together, our work suggests that Spp1 mediates microglia-EC communication in RNV via activating endothelial Kit/Akt/mTOR signaling and is a potential target to treat ischemic ocular diseases.
Plasma Electrolytic Polished Patient-Specific Orbital Implants in Clinical Use—A Technical Note
Authors: Schorn Lara , Wilkat Max , Lommen Julian , Borelli Maria , Muhammad Sajjad , Rana Majeed
Published: January 2023

This technical note describes the technique of plasma electrolytic polishing on orbital patient-specific implants and demonstrates clinical handling and use by the insertion of a plasma electrolytic polished orbital implant into a patient.
Superabsorbent Polymer Use in Rangeland Restoration: Glasshouse Trials
Authors: Nelson Shannon V. , Hansen Neil C. , Madsen Matthew D. , Anderson Val Jo , Eggett Dennis L. , Hopkins Bryan G.
Published: January 2023

Post-disturbance rangeland restoration efforts are often thwarted due to soil moisture deficits. Superabsorbent polymers (SAPs) absorb hundreds of times their weight in water, increasing soil moisture when the SAP is mixed with soil. The objective of this study was to evaluate banded SAPs under the soil surface to increase plant available water and thus seedling establishment for perennial rangeland species during restoration efforts. Five glasshouse experiments with two rangeland perennial grass species, bottlebrush squirreltail (Elymus elymoides) or Siberian wheatgrass (Agropyron fragile), were conducted. Treatments varied, including SAP rates ranging from 11–3000 kg ha−1 with placement mostly banded at depths extending from the surface up to a 15 cm depth. Generally, SAPs increased soil moisture at all rates and depths for up to 49 days. However, rates ≥ 750 kg ha−1 caused the soil to swell and crack, potentially hastening soil drying later in the season. Seedling longevity was increased up to 12 days, especially at the high SAP band rate of 3000 kg ha−1 when the band was 8 or 15 cm deep. Further work is needed to verify banded SAP rates and placement depths in the field, ascertain conditions to reduce soil displacement, and evaluate benefits across species.
Co-Composted Chicken Litter Biochar Increases Soil Nutrient Availability and Yield of Oryza sativa L.
Authors: Ali Maru , Ahmed Osumanu Haruna , Jalloh Mohamadu Boyie , Primus Walter Charles , Musah Adiza Alhassan , Ng Ji Feng
Published: January 2023

Intensified cultivation of rice has accelerated weathering of most tropical acid soils leading to significant loss of base cations. In most developing countries, rice yield is low and this results in its production being costly because productivity versus labor is low. The objectives of this study were to (i) enhance soil chemical properties, nutrient uptake, and grain yield of rice grown on a mineral tropical acid soil using agro-wastes; (ii) determine the agro-waste (chicken manure, cow dung, forest litter, and Leucaena) that has the potential to significantly increase rice yield; and (iii) determine the residual effects of the organic soil amendments produced from the agro-wastes on soil and rice productivity. The treatments used in this three-cycle field study were (i) soil without amendments (S0); (ii) prevailing recommended rates for fertilizers (NPK-Mg); (iii) biochar–forest litter compost (OSA1); (iv) biochar–chicken litter compost (OSA2); (v) biochar–cow dung compost (OSA3); (vi) biochar–Leucaena compost (OSA4); and (vii) biochar–Leucaena–chicken litter compost (OSA5). Standard procedures were used to determine the plants’ rice growth, grain yield, plant nutrient concentrations and uptake, and selected soil chemical properties. The use of organic soil amendments (OSA1 to OSA5) significantly improved the soil chemical properties, rice plant growth, nutrient uptake, and grain yield compared with the prevailing method of cultivating rice (NPK-Mg). The application of organic soil amendments reduced the use of inorganic N, P, K, MgO, and trace elements fertilizers up to 25%, 100%, 64%, 100%, and 100%, respectively. The organic soil amendments with Leucaena significantly increased rice grain yield of OSA5 at 11.17, 13.11, and 10.06 t ha−1 in the first, second, and third cropping cycles, respectively. The residual effect of the organic soil amendments also improved rice plant growth, nutrient uptake, and rice grain yield although these were slightly reduced as compared to those of the two previous cropping cycles, the afore-stated treatments were superior to the prevailing method of cultivating rice (NPK-Mg). Transforming agro-wastes into organic soil amendments can improve tropical mineral acid soils and rice productivity.
Claiming Justice: An Analysis of Child Sexual Abuse Complainants’ Justice Goals Reported during Investigative Interviews
Authors: Holder Robyn L. , Gerryts Dirkje , Garcia Francisco , Powell Martine
Published: January 2023

Investigative interviewing of children who report sexual victimisation focuses on helping children tell in their own words what happened. Children may say other things important to them such as their justice goals. We conducted the first research into this possibility in an exploratory analysis of 300 transcripts of actual interviews with child complainants aged 3 to 15 years. Building on an earlier study involving adults, we explored what goals children may articulate, when in the interview process their goals are relayed and in response to which interviewer prompts. Our analysis revealed that most children did articulate one or more justice goals during these interviews, especially their desire for acknowledgement of the victimisation and its wrongfulness. Children articulated their justice goals spontaneously and largely without any direct prompting by the police officer. These findings suggest that there is more that institutions [and researchers] can learn from carefully listening to children and understanding them as agents claiming justice.
The Impact of COVID-19 Pandemic on Surgical Treatment of Resectable Non-Small Cell Lung Cancer in Greece
Authors: Tomos Ioannis , Kapetanakis Emmanouil I. , Dimakopoulou Konstantina , Raptakis Thomas , Kampoli Katerina , Karakatsani Anna , Koumarianou Anna , Papiris Spyros , Tomos Periklis
Published: January 2023

Background: The coronavirus disease (COVID-19) pandemic has posed an unprecedented challenge to health systems, and has significantly affected the healthcare of lung cancer patients. The aim of our study was to assess the impact of COVID-19 on early lung cancer patients’ surgical treatment. Methods: All consecutive patients with early-stage non-small cell lung cancer eligible for surgical treatment stage I/II and resectable stage III, referred to our department during the first wave of COVID-19 between February to May 2020, were included and compared with those on the exact corresponding quarter in 2019, one year before the pandemic. Waiting time to surgical treatment, increase of tumor’s size and increase on lung cancer stage were recorded and compared. All subjects were followed up for 12 months. Multiple linear and logistic regression models were applied to assess the differences in the management of the studied groups adjusting for potential confounders. Results: Sixty-one patients with early-stage lung cancer were included in the study; 28 (median age 67 years, SD: 7.1) during the pandemic and 33 (median age 67.1 years, SD: 7.5) one year earlier. A significantly longer period of waiting for treatment and an increase in tumor size were observed during the pandemic compared to before the pandemic [median time 47 days, interquartile rate (IQR): 23–100] vs. [median time 18 days, IQR: 11–23], p < 0.001. No significant differences were detected in the increase of the stage of lung cancer between the subgroups. Conclusion: The COVID-19 pandemic had a significant impact on surgical and oncological care, leading to significant delays on treatment and an increase in tumor size in early-stage lung cancer patients.
Metastatic Renal Cell Carcinoma to the Soft Tissue 27 Years after Radical Nephrectomy: A Case Report
Authors: Marra Caterina , Losco Luigi , Ceccaroni Alessandra , Pentangelo Paola , Troisi Donato , Alfano Carmine
Published: January 2023

Background and Objectives: Approximately 20–40% of patients affected with renal cell carcinoma (RCC) develop either distant metastatic or locally recurring disease following radical nephrectomy. Soft tissue, skin, and the central nervous system are less common metastatic sites. We present the case of a patient who has received a diagnosis of RCC; it was found that she had no metastases at the time of nephrectomy but had metastases in the soft tissue and subcutaneous tissue of the scalp 27 years later. As far as we can tell, this is the longest period elapsed between primary renal tumor and subcutaneous/soft tissue metastasis; moreover, this case is the first report of a combined soft tissue/subcutaneous metastasis from RCC. Case presentation: A 73-year-old woman underwent right radical nephrectomy 27 years earlier for clear cell renal cell carcinoma (CCRCC). She presented at our unit because she noticed swelling in the left temporal region; after radiological exams, a benign lesion was suspected. The patient underwent surgical eradication, but the massive bleeding did not allow the removal of the lesion. A biopsy of the mass was performed and the histological examination was consistent with RCC metastases. Conclusions: Metastases from renal cell carcinoma to the subcutaneous and soft tissues are rare. It is essential to take into account RCC metastases in the differential diagnostic of the new starting mass of the head and neck, and the necessity for close and continuous surveillance of patients diagnosed with renal cancer even after a long disease-free period should be emphasized.
Current-Voltage and Transport Characteristics of Heterogeneous Ion-Exchange Membranes in Electrodialysis of Solutions Containing a Heterocyclic Amino Acid and a Strong Electrolyte
Authors: Eliseeva Tatiana , Kharina Anastasiia
Published: January 2023

The alterations in current-voltage and transport characteristics of highly basic and strongly acidic ion-exchange membranes, during the electrodialysis of solutions containing a heterocyclic amino acid and a strong electrolyte, were studied. An increase in the catalytic activity of the water splitting process at the surface of heterogeneous MK-40 and MA-41 membranes upon prolonged contact with proline and tryptophan solutions was found. A significant effect of electroconvection on the components mass transfer through the cation-exchange membrane in the intensive current mode of electrodialysis was revealed for the solution containing a heterocyclic amino acid along with mineral salt (NaCl). This led to a reduction in the length of the “plateau” of the membrane’s current-voltage characteristics, in comparison with the characteristics for an individual sodium chloride solution with the same concentration. The changes in the characteristics of the studied ion-exchange membranes caused by contact with solutions containing heterocyclic amino acids during electrodialysis were reversible when applying electrochemical regeneration (cleaning in place) using the overlimiting current mode, corresponding to the region of facilitated transport for these ampholytes.
A Comparative Differential Scanning Calorimetry Study of Precipitation Hardenable Copper-Based Alloys with Optimized Strength and High Conductivity
Authors: Dölling Julia , Kracun Stefanie Felicia , Prahl Ulrich , Fehlbier Martin , Zilly Andreas
Published: January 2023

Copper alloys with chromium, hafnium, and scandium combining enhanced strength as well as high electrical and thermal conductivity are analyzed in depth. The aim is to compare the precipitation process during temperature exposure to meet increasing material requirements. This research focuses on alloying elements having a limited, maximum 1 wt.%, and with temperature decreasing solubility in copper. For the simultaneous enhancement of mechanical strength and conductivity, precipitation hardening is the utilized mechanism during the processing of as-casted annealed and quenched specimens and in combination with optional cold-rolling prior to the aging process. Extensive DSC measurements, accompanied by metallographic investigations, and the analysis of hardness and electrical conductivity, lead to a versatile description and comparison of different alloying systems. CuCr0.7 starts to precipitate early and is mainly influenced by the temperature of aging. Provoking the solid solution with cold deformation has a less significant influence on the following precipitation. CuSc0.3 and CuHf0.7 precipitate at higher temperatures and are highly influenced by cold deformation prior to aging. Furthermore, CuHf0.7 and CuSc0.3 show advantages regarding the recrystallization behavior, making them especially applicable for higher operating temperatures. Future research will assess ternary alloy combinations to further scoop the potential.
Control of Copper Content in Flash Smelting Slag and the Recovery of Valuable Metals from Slag—A Thermodynamic Consideration
Authors: Xie Sui , Yuan Xinhua , Liu Fupeng , Zhao Baojun
Published: January 2023

To determine slag properties and the factors influencing these properties for optimization of operating conditions in the copper flash smelting process, the composition and microstructures of the quenched smelting and converting slags have been analyzed. Thermodynamic software FactSage 8.2 has been used to investigate the effects of matte grade, SO2 partial pressure, and the Fe/SiO2 ratio on the liquidus temperature and the copper content of the smelting slag. The possibility to recover valuable metals from the smelting and converting slags through pyrometallurgical reduction by carbon is also discussed. It was found that the flash smelting slag temperature is usually higher than its liquidus temperature and the copper (1.2% Cu) is mainly present in the slag as dissolved copper. In the copper flash smelting process, the copper content in the slag can be decreased by decreasing the Fe/SiO2 ratio and temperature. In pyrometallurgical slag reduction, most Cu, Mo, and Ni can be recovered as an alloy. The conditions of recovery such as the ratio of smelting slag to converting slag, temperature, and reduction extent have been discussed.
New Markers for Cardiovascular Disease in Psoriatic Patients: Preliminary Study on Monocyte Phenotype, ADAMTS7, and mTOR Activity
Authors: Loyola Khanty , Karsulovic Claudio , Cabrera Raúl , Perez Claudio , Hojman Lía
Published: January 2023

Psoriasis is a skin disease with occasional involvement of non-cutaneous territories. Beyond the usual, cardiovascular events are more frequent in these patients and correlate only partially with disease activity, suggesting the presence of other unknown factors. We selected ten psoriatic patients without treatment in the last year and matched them for age and gender with eleven healthy subjects. Ficoll-extracted mononuclear cells were analyzed with flow cytometry for monocyte surface phenotype markers, intracellular NFκB/inflammasome-dependent interleukins, and chemotaxis receptor CXCR3. Using ELISA, patient serum was evaluated for ADAMTS7 and CXCL10. Inflammatory M1 monocytes showed higher levels of IL-1β and IL-6 in psoriatic patients. M2 monocytes also showed higher levels of intracellular inflammatory cytokines. Nevertheless, IL-6 values were higher compared to other monocytes and IL-1β. The mTORC activation markers ADAMTS7 and S6Rp were higher in psoriatic patients than in healthy controls. In psoriatic patients, serum levels of ADAMTS7 were elevated, and M2 monocytes showed a distinct inflammatory response with higher relative levels of NFκB-dependent IL-6 and less activity of the CXCR3–CXCL10 chemotactic pathway. These data suggest pathways with potential markers for prediction and early detection of cardiovascular risk in psoriatic patients.
Elevation of Lipid Metabolites in Deceased Liver Donors Reflects Graft Suffering
Authors: Lee Wei-Chen , Wu Ting-Jung , Cheng Chih-Hsien , Wang Yu-Chao , Hung Hao-Chien , Lee Jin-Chiao , Wu Tsung-Han , Chou Hong-Shiue , Lee Chen-Fang , Chan Kun-Ming
Published: January 2023

Liver transplantation can be performed with deceased or living donor allografts. Deceased liver grafts are donated from brain- or circulation-death patients, and they have usually suffered from a certain degree of damage. Post-transplant graft function and patient survival are closely related to liver allograft recovery. How to define the damage of liver grafts is unclear. A total of 47 liver donors, 23 deceased and 24 living, were enrolled in this study. All deceased donors had suffered from severe brain damage, and six of them had experienced cardio-pulmonary-cerebral resuscitation (CPR). The exploration of liver graft metabolomics was conducted by liquid chromatography coupled with mass spectrometry. Compared with living donor grafts, the deceased liver grafts expressed higher levels of various diacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, oleoylcarnitine and linoleylcarnitine; and lower levels of cardiolipin and phosphatidylcholine. The liver grafts from the donors with CPR had higher levels of cardiolipin, phosphatidic acid, phosphatidylcholine, phatidylethanolamine and amiodarone than the donors without CPR. When focusing on amino acids, the deceased livers had higher levels of histidine, taurine and tryptophan than the living donor livers. In conclusion, the deceased donors had suffered from cardio-circulation instability, and their lipid metabolites were increased. The elevation of lipid metabolites can be employed as an indicator of liver graft suffering.