bluuebunny/crossref_metadata_2025 huggingface dataset:
Wavelike Oscillations in High Latitude Thermospheric Doppler Temperature and Line‐Of‐Sight Wind Observed Using All‐Sky Imaging Fabry‐Perot Spectrometers
Authors: Itani R. , Conde M.
Published: September 2023

Multiple years of thermospheric wind and temperature data were examined to study gravity waves in Earth's thermosphere. Winds and temperatures were measured using all‐sky imaging optical Doppler spectrometers deployed at two sites in Alaska, and three in Antarctica. For all sites, oscillatory perturbations were clearly present in high‐pass temporally filtered F‐region line‐of‐sight (LOS) winds for the majority of the clear‐sky nights. Oscillations were also discernible in E‐region LOS wind and F‐region Doppler temperature, albeit less frequently. Oscillation amplitudes correlated strongly with auroral and geomagnetic activity. Observed wave signatures also correlated strongly between geographically nearby observing sites. Amplitudes of LOS wind oscillations were usually small when viewed in the zenith and increased approximately with the sine of the zenith angle—as expected if the underlying motion is predominantly horizontal. Scanning Doppler Imager instruments observe in many look directions simultaneously. Phase relationships between perturbations observed in different look directions were used to identify time intervals when the oscillations were likely to be due to traveling waves. However, a number of instances were noted in which the oscillations had characteristics suggesting geophysical mechanisms other than traveling waves—a recognition that was only possible because of the large number of look directions. Lomb‐Scargle analysis was used on a representative subset of days to resolve the spectral distributions of the wind and temperature oscillations. F‐region wind oscillations on days analyzed this way exhibited periods typically ranging from 60 min and above. By contrast, E‐region wind oscillation periods were as short as 30 min.
What Are the Similarities and Differences in Marine Boundary Layer Cloud and Drizzle Microphysical Properties During the ACE‐ENA and MARCUS Field Campaigns?
Authors: Marcovecchio Alexa. R. , Xi Baike , Zheng Xiaojian , Wu Peng , Dong Xiquan , Behrangi Ali
Published: September 2023

This study compares macrophysical and microphysical properties of single‐layered, liquid‐dominant MBL clouds from the Measurements of Aerosols, Radiation, and Clouds over the Southern Ocean (MARCUS) (above 60°S) and the ARM East North Atlantic (ENA) site during the Aerosol and Cloud Experiments in Eastern North Atlantic (ACE‐ENA) field campaign. A total of 1,136 (16.5% of clouds) and 6,034 5‐min cloud samples are selected from MARCUS and ARM ENA in this study. MARCUS clouds have higher cloud‐top heights, thicker cloud layers, larger liquid water path, and colder cloud temperatures than ENA. Thinner, warmer MBL clouds at ENA can contain higher layer‐mean liquid water content due to higher cloud and ocean surface temperatures along with greater precipitable water vapor (PWV). MARCUS has a higher drizzle frequency rate (71.8%) than ENA (45.1%). Retrieved cloud and drizzle microphysical properties from each field campaign show key differences. MARCUS clouds feature smaller cloud droplets, whereas ENA clouds have larger cloud droplets, especially at the upper region of the cloud. From cloud top to cloud base, drizzle drop sizes increase while number concentrations decrease. Drizzle drop radius and number concentration decrease from cloud base to drizzle base due to net evaporation, and MARCUS' lower specific humidity leads to a higher drizzle base than ENA. The broader surface pressure and lower tropospheric stability (LTS) distributions during MARCUS have demonstrated that there are different synoptic patterns for selected cases during MARCUS with less PWV, while ENA is dominated by high pressure systems with nearly doubled PWV.
Forecasting the 2016–2017 Central Apennines Earthquake Sequence With a Neural Point Process
Authors: Stockman Samuel , Lawson Daniel J. , Werner Maximilian J.
Published: September 2023

Point processes have been dominant in modeling the evolution of seismicity for decades, with the epidemic‐type aftershock sequence (ETAS) model being most popular. Recent advances in machine learning have constructed highly flexible point process models using neural networks to improve upon existing parametric models. We investigate whether these flexible point process models can be applied to short‐term seismicity forecasting by extending an existing temporal neural model to the magnitude domain and we show how this model can forecast earthquakes above a target magnitude threshold. We first demonstrate that the neural model can fit synthetic ETAS data, however, requiring less computational time because it is not dependent on the full history of the sequence. By artificially emulating short‐term aftershock incompleteness in the synthetic data set, we find that the neural model outperforms ETAS. Using a new enhanced catalog from the 2016–2017 Central Apennines earthquake sequence, we investigate the predictive skill of ETAS and the neural model with respect to the lowest input magnitude. Constructing multiple forecasting experiments using the Visso, Norcia and Campotosto earthquakes to partition training and testing data, we target M3+ events. We find both models perform similarly at previously explored thresholds (e.g., above M3), but lowering the threshold to M1.2 reduces the performance of ETAS unlike the neural model. We argue that some of these gains are due to the neural model's ability to handle incomplete data. The robustness to missing data and speed to train the neural model present it as an encouraging competitor in earthquake forecasting.
The Social Cost of Ozone‐Related Mortality Impacts From Methane Emissions
Authors: McDuffie Erin E. , Sarofim Marcus C. , Raich William , Jackson Melanie , Roman Henry , Seltzer Karl , Henderson Barron H. , Shindell Drew T. , Collins Mei , Anderton Jim , Barr Sarah , Fann Neal
Published: September 2023

Atmospheric methane directly affects surface temperatures and indirectly affects ozone, impacting human welfare, the economy, and environment. The social cost of methane (SC‐CH4) metric estimates the costs associated with an additional marginal metric ton of emissions. Current SC‐CH4 estimates do not consider the indirect impacts associated with ozone production from changes in methane. We use global model simulations and a new BenMAP webtool to estimate respiratory‐related deaths associated with increases in ozone from a pulse of methane emissions in 2020. By using an approach consistent with the current SC‐CH4 framework, we monetize and discount annual damages back to present day values. We estimate that the methane‐ozone mechanism is attributable to 760 (95% CI: 330–1200) respiratory‐related deaths per million metric tons of methane globally, for a global net present damage of $1800/mT (95% CI: $760–$2800/mT CH4; 2% Ramsey discount rate); this would double the current SC‐CH4 if included. These physical impacts are consistent with recent studies, but comparing direct costs is challenging. Economic damages are sensitive to uncertainties in the exposure and health risks associated with tropospheric ozone, assumptions about future projections of NOx emissions, socioeconomic conditions, and mortality rates, monetization parameters, and other factors. Our estimates are highly sensitive to uncertainties in ozone health risks. We also develop a reduced form model to test sensitivities to other parameters. The reduced form tool runs with a user‐supplied emissions pulse, as well as socioeconomic and precursor projections, enabling future integration of the methane‐ozone mechanism into the SC‐CH4 modeling framework.
Dynamics of Mid‐Latitude Sporadic‐E and Its Impact on HF Propagation in the North American Sector
Authors: Kunduri B. S. R. , Erickson P. J. , Baker J. B. H. , Ruohoniemi J. M. , Galkin I. A. , Sterne K. T.
Published: September 2023

Sporadic‐E (Es) are thin layers of enhanced ionization observed in the E‐region, typically between 95 and 120 km altitude. Es plays an important role in controlling the dynamics of the upper atmosphere and it is necessary to understand the geophysical factors influencing Es from both the scientific and operational perspectives. While the wind‐shear theory is widely accepted as an important mechanism responsible for the generation of Es, there are still gaps in the current state of our knowledge. For example, we are yet to determine precisely how changes in the dynamics of horizontal winds impact the formation, altitude, and destruction of Es layers. In this study, we report results from a coordinated experimental campaign between the Millstone Hill Incoherent Scatter Radar, the SuperDARN radar at Blackstone, and the Millstone Hill Digisonde to monitor the dynamics of mid‐latitude Es layers. We report observations during a 15‐hr window between 13 UT on 3 June 2022 and 4 UT on 4 June 2022, which was marked by the presence of a strong Es layer. We find that the height of the Es layer is collocated with strong vertical shears in atmospheric tides and that the zonal wind shears play a more important role than meridional wind shears in generating Es, especially at lower altitudes. Finally, we show that in the presence of Es, SuperDARN ground backscatter moves to closer ranges, and the height and critical frequency of the Es layer have a significant impact on the location and intensity of HF ground scatter.
Electron Heating in Shocks: Statistics and Comparison
Authors: Gedalin Michael , Golan Michal , Vink Jacco , Ganushkina Natalia , Balikhin Michael
Published: September 2023

Supernova remnant (SNR) shocks are the highest Mach number non‐relativistic shocks in electron‐ion plasmas. These shocks are the most efficient particle accelerators in space. SNR shock parameters are inferred from measurements of electromagnetic radiation from heated and accelerated particles. Temperature of the shock heated electrons is one of the most important parameters in supernova remnant shocks. Knowledge of the downstream electron‐to‐ion temperature ratio or of the ratio of the downstream electron temperature to the incident ion energy is crucial for understanding physics of the very high‐Mach number SNR shocks. Heliospheric shocks have substantially lower Mach numbers than SNR shocks but can be extensively studied in in situ observations with further extrapolation of the findings to higher Mach numbers. Magnetospheric Multiscale mission observations of the Earth bow shock are used to analyze dependence of the electron heating on the shock Mach number. It is found that the ratio of the downstream electron temperature to the incident ion energy decreases with the increase of the Mach number. At high Mach numbers this ratio and stabilizes at about 2.5%. The electron‐to‐ion temperature ratio stabilizes at about 10%. The peak electron temperature occurs at the overshoot maximum, further downstream electrons cool down. The mean ratio of the 4.5 s averages of the downstream and maximum electron temperatures is 0.85. Electron heating does not follow the thermodynamic adiabatic law. The heating and cooling behavior implies that the energy is provided by the overall cross‐shock potential while small‐scale electric fields rapidly isotropize the electron distribution.
Seismicity and Magmatic System of the Changbaishan Intraplate Volcano in East Asia
Authors: Yan Dong , Tian You , Zhao Dapeng , Li Hongli
Published: September 2023

A high‐precision earthquake catalog is made from continuous waveform data recorded at 360 portable seismographs deployed for one month in the Changbaishan‐Tianchi volcanic area based on an automatic earthquake detection and location workflow. Then we investigate detailed 3‐D structures of P and S wave velocities (Vp and Vs) and Vp/Vs ratio of the crust beneath the study region. Our results reveal a high‐Vp/Vs zone accompanied with active volcanic earthquakes beneath the Tianchi caldera at ∼5 km depth, which may reflect a magma chamber. The intense seismic activities within 2 km depth beneath the Tianchi caldera from 2002 to 2005 occurred directly above the magma chamber revealed by this study, which could be related to upward migration of high‐pressure volatiles originating from the magma chamber. A potential middle‐crustal magma chamber might provide continuous magmatic materials feeding the upper‐crust magma chamber and lead to ruptures of surrounding rocks, resulting in two swarms of volcanic seismicity during the observation period. We deem that the diverse eruption styles of the Changbaishan volcano are closely associated with the multilevel structure of magma chambers in the upper and middle crust.
Pesticide, allergen, PCB, and lead measurements in childcare centers located on tribal lands in the Pacific Northwest, United States
Authors: Tulve Nicolle S. , Croghan Carry W. , Plewe Bethany L. , Thompson Duffy Holly , Adams Katie , McBride Theresa , Pace Christopher , Wood Doug , Fish Christopher
Published: September 2023

Background

Children’s potential exposures to chemical and biological agents in tribal childcare centers are not well characterized.

Objectives

(1) The environmental health of childcare centers in Portland Area Indian Country was characterized by measuring selected pesticides, polychlorinated biphenyls (PCBs), allergens, and lead (Pb) in outdoor soil and indoor dust. (2) We compared our results to other studies of childcare centers in both the United States and globally.

Methods

At 31 tribal childcare centers in Washington, Oregon, and Idaho, we collected indoor dust and outdoor soil samples from at least one classroom, multipurpose room, and outdoor play area. Number of rooms sampled depended on facility size. Surface wipes were collected from the floor, play/work surface, and windowsill and analyzed for selected pesticides and PCBs. Vacuum samples were collected from the floor and analyzed for selected allergens. Lead was measured in surface wipes and outdoor soil collected at 11 centers. A questionnaire collected information on demographics, cleaning habits, and pesticide usage.

Results

At least one pesticide was measured at all childcare centers. cis-Permethrin (surface wipes: 0.003–180 ng/cm2), trans-permethrin (surface wipes: 0.002–200 ng/cm2) and piperonyl butoxide (surface wipes: 0.001–120 ng/cm2) were measured in all centers. Lead was measured in most surface wipes (<0.25–14 ng/cm2) and all outdoor soil samples (8.4–50 mg/kg). Aroclors 1242 and 1254 were detected on indoor surfaces in three centers at very low loadings. Allergen residues were measured at very low concentrations in vacuum dust samples (Der p 1: <0.012–0.12 µg/g; Der f 1: <0.012–0.09 µg/g; Mus m 1: <0.002–10.055 µg/g). In general, we observed lower levels of chemical and biological agents than what has been reported previously.

Significance

By understanding the environmental health of childcare centers, we can better understand the role of child-specific environments in promoting children’s health and well-being.

Impact statement

To our knowledge, this is the first study to characterize the environmental health of tribal childcare centers in the Pacific Northwest. Combined with the information we have on childcare centers from around the world, this study expands our knowledge on young children’s potential exposures to chemical and biological agents in locations where they spend significant amounts of time.
Manipulating nonlinear exciton polaritons in an atomically-thin semiconductor with artificial potential landscapes
Authors: Luo Yuan , Guo Quanbing , Deng Xinyi , Ghosh Sanjib , Zhang Qing , Xu Hongxing , Xiong Qihua
Published: September 2023

Exciton polaritons in atomically thin transition-metal dichalcogenide microcavities provide a versatile platform for advancing optoelectronic devices and studying the interacting Bosonic physics at ambient conditions. Rationally engineering the favorable properties of polaritons is critically required for the rapidly growing research. Here, we demonstrate the manipulation of nonlinear polaritons with the lithographically defined potential landscapes in monolayer WS2 microcavities. The discretization of photoluminescence dispersions and spatially confined patterns indicate the deterministic on-site localization of polaritons by the artificial mesa cavities. Varying the trapping sizes, the polariton-reservoir interaction strength is enhanced by about six times through managing the polariton–exciton spatial overlap. Meanwhile, the coherence of trapped polaritons is significantly improved due to the spectral narrowing and tailored in a picosecond range. Therefore, our work not only offers a convenient approach to manipulating the nonlinearity and coherence of polaritons but also opens up possibilities for exploring many-body phenomena and developing novel polaritonic devices based on 2D materials.
Circular RNA vaccine in disease prevention and treatment
Authors: Niu Dun , Wu Yaran , Lian Jiqin
Published: September 2023

CircRNAs are a class of single-stranded RNAs with covalently linked head-to-tail topology. In the decades since its initial discovery, their biogenesis, regulation, and function have rapidly disclosed, permitting a better understanding and adoption of them as new tools for medical applications. With the development of biotechnology and molecular medicine, artificial circRNAs have been engineered as a novel class of vaccines for disease treatment and prevention. Unlike the linear mRNA vaccine which applications were limited by its instability, inefficiency, and innate immunogenicity, circRNA vaccine which incorporate internal ribosome entry sites (IRESs) and open reading frame (ORF) provides an improved approach to RNA-based vaccination with safety, stability, simplicity of manufacture, and scalability. However, circRNA vaccines are at an early stage, and their optimization, delivery and applications require further development and evaluation. In this review, we comprehensively describe circRNA vaccine, including their history and superiority. We also summarize and discuss the current methodological research for circRNA vaccine preparation, including their design, synthesis, and purification. Finally, we highlight the delivery options of circRNA vaccine and its potential applications in diseases treatment and prevention. Considering their unique high stability, low immunogenicity, protein/peptide-coding capacity and special closed-loop construction, circRNA vaccine, and circRNA-based therapeutic platforms may have superior application prospects in a broad range of diseases.
Antifungal susceptibility profiles for fungal isolates from corneas and contact lenses in the United Kingdom
Authors: Tuft Stephen , Stone Neil R. H. , Burton Matthew J. , Johnson Elizabeth M. , Borman Andrew M.
Published: September 2023

Objective

To report the identification and results of susceptibility testing for fungal isolates from the cornea or contact lens care systems.

Materials and methods

In this retrospective epidemiological study, we searched the results of fungal cultures from cornea or contact lens systems referred for identification and susceptibility testing to the United Kingdom National Mycology Reference Laboratory between October 2016 and March 2022. For each fungal isolate, we recorded the genus and species of the fungus and the minimum inhibitory concentration (MIC) to six antifungal agents available to treat corneal infection (amphotericin, econazole, itraconazole, natamycin, posaconazole, and voriconazole).

Results

There were 600 isolates from 585 patients, comprising 374 (62%) from corneal samples and 226 from contact lenses and care systems, of which 414 (69%) isolates were moulds (filamentous fungi) and 186 (31%) were yeasts. The most frequent moulds isolated were Fusarium spp (234 isolates, 39%) and Aspergillus spp (62, 10%). The most frequent yeasts isolated were Candida spp (112, 19%), predominantly Candida parapsilosis (65, 11%) and Candida albicans (33, 6%), with 35 isolates (6%) of Meyerozyma guilliermondii. In vitro susceptibility was greatest for natamycin (347 moulds tested, mode 4 mg/L, range 0.25–64 mg/L; 98 yeasts tested, mode 4 mg/L, range 0.5–32 mg/L), with susceptibility for 94% for moulds and 99% yeasts. Of the 16 isolates interpreted as highly resistant to natamycin (MIC ≥16 mg/L), 13 were Aspergillus flavus complex.

Conclusions

In vitro susceptibility supports the use of natamycin for the empiric treatment of fungal keratitis in the UK.
Novel splice site variant of TMEM38B in osteogenesis imperfecta type XIV
Authors: Kodama Yoshihiko , Meiri Satoru , Asada Tomoko , Matsuyama Misayo , Makino Shinya , Iwai Minayo , Yamaguchi Masatoshi , Moritake Hiroshi
Published: September 2023

Osteogenesis imperfecta (OI) is a rare genetic disorder characterized by brittle bones. In this case report, we describe a patient who suffered from OI type XIV with a novel splice site variant in the TMEM38B gene. Further research is needed to better understand the relationship between the phenotype of OI type XIV and this variant.
Enhanced thermally-activated skyrmion diffusion with tunable effective gyrotropic force
Authors: Dohi Takaaki , Weißenhofer Markus , Kerber Nico , Kammerbauer Fabian , Ge Yuqing , Raab Klaus , Zázvorka Jakub , Syskaki Maria-Andromachi , Shahee Aga , Ruhwedel Moritz , Böttcher Tobias , Pirro Philipp , Jakob Gerhard , Nowak Ulrich , Kläui Mathias
Published: September 2023

Magnetic skyrmions, topologically-stabilized spin textures that emerge in magnetic systems, have garnered considerable interest due to a variety of electromagnetic responses that are governed by the topology. The topology that creates a microscopic gyrotropic force also causes detrimental effects, such as the skyrmion Hall effect, which is a well-studied phenomenon highlighting the influence of topology on the deterministic dynamics and drift motion. Furthermore, the gyrotropic force is anticipated to have a substantial impact on stochastic diffusive motion; however, the predicted repercussions have yet to be demonstrated, even qualitatively. Here we demonstrate enhanced thermally-activated diffusive motion of skyrmions in a specifically designed synthetic antiferromagnet. Suppressing the effective gyrotropic force by tuning the angular momentum compensation leads to a more than 10 times enhanced diffusion coefficient compared to that of ferromagnetic skyrmions. Consequently, our findings not only demonstrate the gyro-force dependence of the diffusion coefficient but also enable ultimately energy-efficient unconventional stochastic computing.
Extracellular calcium functions as a molecular glue for transmembrane helices to activate the scramblase Xkr4
Authors: Zhang Panpan , Maruoka Masahiro , Suzuki Ryo , Katani Hikaru , Dou Yu , Packwood Daniel M. , Kosako Hidetaka , Tanaka Motomu , Suzuki Jun
Published: September 2023

The “eat me” signal, phosphatidylserine is exposed on the surface of dying cells by phospholipid scrambling. Previously, we showed that the Xkr family protein Xkr4 is activated by caspase-mediated cleavage and binding of the XRCC4 fragment. Here, we show that extracellular calcium is an additional factor needed to activate Xkr4. The constitutively active mutant of Xkr4 is found to induce phospholipid scrambling in an extracellular, but not intracellular, calcium-dependent manner. Importantly, other Xkr family members also require extracellular calcium for activation. Alanine scanning shows that D123 and D127 of TM1 and E310 of TM3 coordinate calcium binding. Moreover, lysine scanning demonstrates that the E310K mutation-mediated salt bridge between TM1 and TM3 bypasses the requirement of calcium. Cysteine scanning proves that disulfide bond formation between TM1 and TM3 also activates phospholipid scrambling without calcium. Collectively, this study shows that extracellular calcium functions as a molecular glue for TM1 and TM3 of Xkr proteins for activation, thus demonstrating a regulatory mechanism for multi-transmembrane region-containing proteins.
A split and inducible adenine base editor for precise in vivo base editing
Authors: Zeng Hongzhi , Yuan Qichen , Peng Fei , Ma Dacheng , Lingineni Ananya , Chee Kelly , Gilberd Peretz , Osikpa Emmanuel C. , Sun Zheng , Gao Xue
Published: September 2023

DNA base editors use deaminases fused to a programmable DNA-binding protein for targeted nucleotide conversion. However, the most widely used TadA deaminases lack post-translational control in living cells. Here, we present a split adenine base editor (sABE) that utilizes chemically induced dimerization (CID) to control the catalytic activity of the deoxyadenosine deaminase TadA-8e. sABE shows high on-target editing activity comparable to the original ABE with TadA-8e (ABE8e) upon rapamycin induction while maintaining low background activity without induction. Importantly, sABE exhibits a narrower activity window on DNA and higher precision than ABE8e, with an improved single-to-double ratio of adenine editing and reduced genomic and transcriptomic off-target effects. sABE can achieve gene knockout through multiplex splice donor disruption in human cells. Furthermore, when delivered via dual adeno-associated virus vectors, sABE can efficiently convert a single A•T base pair to a G•C base pair on the PCSK9 gene in mouse liver, demonstrating in vivo CID-controlled DNA base editing. Thus, sABE enables precise control of base editing, which will have broad implications for basic research and in vivo therapeutic applications.
Fluidic shaping and in-situ measurement of liquid lenses in microgravity
Authors: Luria Omer , Elgarisi Mor , Frumkin Valeri , Razin Alexey , Ericson Jonathan , Gommed Khaled , Widerker Daniel , Gabay Israel , Belikov Ruslan , Bookbinder Jay , Balaban Edward , Bercovici Moran
Published: September 2023

In the absence of gravity, surface tension dominates over the behavior of liquids. While this often poses a challenge in adapting Earth-based technologies to space, it can also provide an opportunity for novel technologies that utilize its advantages. In particular, surface tension drives a liquid body to a constant-mean-curvature shape with extremely smooth surfaces, properties which are highly beneficial for optical components. We here present the design, implementation and analysis of parabolic flight experiments demonstrating the creation and in-situ measurement of optical lenses made entirely by shaping liquids in microgravity. We provide details of the two experimental systems designed to inject the precise amount of liquid within the short microgravity timeframe provided in a parabolic flight, while also measuring the resulting lens’ characteristics in real-time using both resolution target-imaging and Shack-Hartmann wavefront sensing. We successfully created more than 20 liquid lenses during the flights. We also present video recordings of the process, from the lenses’ creation during microgravity and up until their collapse upon return to gravity. The work thus demonstrates the feasibility of creating and utilizing liquid-based optics in space.
Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs
Authors: Yu Chunlai , Zhang Mei , Xiong Yucui , Wang Qizheng , Wang Yuanhua , Wu Shaoling , Hussain Sajjad , Wang Yan , Zhang Zhizhong , Rao Nini , Zhang Sheng , Zhang Xiao
Published: September 2023

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) through epigenetic manipulation. While the essential role of miRNA in reprogramming and maintaining pluripotency is well studied, little is known about the functions of miRNA from exosomes in this context. To fill this research gap,we comprehensively obtained the 17 sets of cellular mRNA transcriptomic data with 3.93 × 1010 bp raw reads and 18 sets of exosomal miRNA transcriptomic data with 2.83 × 107 bp raw reads from three categories of human somatic cells: peripheral blood mononuclear cells (PBMCs), skin fibroblasts(SFs) and urine cells (UCs), along with their derived iPSCs. Additionally, differentially expressed molecules of each category were identified and used to perform gene set enrichment analysis. Our study provides sets of comparative transcriptomic data of cellular mRNA and exosomal miRNA from three categories of human tissue with three individual biological controls in studies of iPSCs generation, which will contribute to a better understanding of donor cell variation in functional epigenetic regulation and differentiation bias in iPSCs.
EWELD: A Large-Scale Industrial and Commercial Load Dataset in Extreme Weather Events
Authors: Liu Guolong , Liu Jinjie , Bai Yan , Wang Chengwei , Wang Haosheng , Zhao Huan , Liang Gaoqi , Zhao Junhua , Qiu Jing
Published: September 2023

Load forecasting is crucial for the economic and secure operation of power systems. Extreme weather events, such as extreme heat and typhoons, can lead to more significant fluctuations in power consumption, making load forecasting more difficult. At present, due to the lack of relevant public data, the research on load forecasting under extreme weather events is still blank, so it is necessary to release a large-scale load dataset containing extreme weather events. The dataset includes electricity consumption data of industrial and commercial users under extreme weather events such as typhoons and extreme heat, which are collected at 15-minute intervals. The data is collected over six years from smart meters installed at the power entry points of users in southern China. The dataset consists of electricity consumption data from 386 industrial and commercial users in 17 industries, with more than 50 million records. During the recording period, extreme weather events such as typhoons and extreme heat are marked to form a total of 5,741 event records.
Chromosome level genome assembly of oriental armyworm Mythimna separata
Authors: Xu Chao , Ji Jichao , Zhu Xiangzhen , Huangfu Ningbo , Xue Hui , Wang Li , Zhang Kaixin , Li Dongyang , Niu Lin , Chen Ran , Gao Xueke , Luo Junyu , Cui Jinjie
Published: September 2023

The oriental armyworm, Mythimna separata, is an extremely destructive polyphagous pest with a broad host range that seriously threatens the safety of agricultural production. Here, a high-quality chromosome-level genome was assembled using Illumina, PacBio HiFi long sequencing, and Hi-C scaffolding technologies. The genome size was 706.30 Mb with a contig N50 of 22.08 Mb, and 99.2% of the assembled sequences were anchored to 31 chromosomes. In addition, 20,375 protein-coding genes and 258.68 Mb transposable elements were identified. The chromosome-level genome assembly of M. separata provides a significant genetic resource for future studies of this insect and contributes to the development of management strategies.
Global crop-specific nitrogen fertilization dataset in 1961–2020
Authors: Adalibieke Wulahati , Cui Xiaoqing , Cai Hongwei , You Liangzhi , Zhou Feng
Published: September 2023

Nitrogen (N) is an important nutrient for crop growth. However, the overuse of N fertilizers has led to a series of devastating global environmental issues. Recent studies show that multiple datasets have been created for agricultural N fertilizer application with varied temporal or spatial resolutions, nevertheless, how to synchronize and use these datasets becomes problematic due to the inconsistent temporal coverages, spatial resolutions, and crop-specific allocations. Here we reconstructed a comprehensive dataset for crop-specific N fertilization at 5-arc-min resolution (~10 km by 10 km) during 1961–2020, including N application rate, types, and placements. The N fertilization data was segmented by 21 crop groups, 13 fertilizer types, and 2 fertilization placements. Comparison analysis showed that our dataset is aligned with previous estimates. Our spatiotemporal N fertilization dataset could be used for the land surface models to quantify the effects of agricultural N fertilization practices on food security, climate change, and environmental sustainability.
A high-resolution daily global dataset of statistically downscaled CMIP6 models for climate impact analyses
Authors: Gebrechorkos Solomon , Leyland Julian , Slater Louise , Wortmann Michel , Ashworth Philip J. , Bennett Georgina L. , Boothroyd Richard , Cloke Hannah , Delorme Pauline , Griffith Helen , Hardy Richard , Hawker Laurence , McLelland Stuart , Neal Jeffrey , Nicholas Andrew , Tatem Andrew J. , Vahidi Ellie , Parsons Daniel R. , Darby Stephen E.
Published: September 2023

A large number of historical simulations and future climate projections are available from Global Climate Models, but these are typically of coarse resolution, which limits their effectiveness for assessing local scale changes in climate and attendant impacts. Here, we use a novel statistical downscaling model capable of replicating extreme events, the Bias Correction Constructed Analogues with Quantile mapping reordering (BCCAQ), to downscale daily precipitation, air-temperature, maximum and minimum temperature, wind speed, air pressure, and relative humidity from 18 GCMs from the Coupled Model Intercomparison Project Phase 6 (CMIP6). BCCAQ is calibrated using high-resolution reference datasets and showed a good performance in removing bias from GCMs and reproducing extreme events. The globally downscaled data are available at the Centre for Environmental Data Analysis (https://doi.org/10.5285/c107618f1db34801bb88a1e927b82317) for the historical (1981–2014) and future (2015–2100) periods at 0.25° resolution and at daily time step across three Shared Socioeconomic Pathways (SSP2-4.5, SSP5-3.4-OS and SSP5-8.5). This new climate dataset will be useful for assessing future changes and variability in climate and for driving high-resolution impact assessment models.
AmeriFlux BASE data pipeline to support network growth and data sharing
Authors: Chu Housen , Christianson Danielle S. , Cheah You-Wei , Pastorello Gilberto , O’Brien Fianna , Geden Joshua , Ngo Sy-Toan , Hollowgrass Rachel , Leibowitz Karla , Beekwilder Norman F. , Sandesh Megha , Dengel Sigrid , Chan Stephen W. , Santos André , Delwiche Kyle , Yi Koong , Buechner Christin , Baldocchi Dennis , Papale Dario , Keenan Trevor F. , Biraud Sébastien C. , Agarwal Deborah A. , Torn Margaret S.
Published: September 2023

AmeriFlux is a network of research sites that measure carbon, water, and energy fluxes between ecosystems and the atmosphere using the eddy covariance technique to study a variety of Earth science questions. AmeriFlux’s diversity of ecosystems, instruments, and data-processing routines create challenges for data standardization, quality assurance, and sharing across the network. To address these challenges, the AmeriFlux Management Project (AMP) designed and implemented the BASE data-processing pipeline. The pipeline begins with data uploaded by the site teams, followed by the AMP team’s quality assurance and quality control (QA/QC), ingestion of site metadata, and publication of the BASE data product. The semi-automated pipeline enables us to keep pace with the rapid growth of the network. As of 2022, the AmeriFlux BASE data product contains 3,130 site years of data from 444 sites, with standardized units and variable names of more than 60 common variables, representing the largest long-term data repository for flux-met data in the world. The standardized, quality-ensured data product facilitates multisite comparisons, model evaluations, and data syntheses.
Continuous peripersonal tracking accuracy is limited by the speed and phase of locomotion
Authors: Davidson Matthew J. , Keys Robert Tobin , Szekely Brian , MacNeilage Paul , Verstraten Frans , Alais David
Published: September 2023

Recent evidence suggests that perceptual and cognitive functions are codetermined by rhythmic bodily states. Prior investigations have focused on the cardiac and respiratory rhythms, both of which are also known to synchronise with locomotion—arguably our most common and natural of voluntary behaviours. Compared to the cardiorespiratory rhythms, walking is easier to voluntarily control, enabling a test of how natural and voluntary rhythmic action may affect sensory function. Here we show that the speed and phase of human locomotion constrains sensorimotor performance. We used a continuous visuo-motor tracking task in a wireless, body-tracking virtual environment, and found that the accuracy and reaction time of continuous reaching movements were decreased at slower walking speeds, and rhythmically modulated according to the phases of the step-cycle. Decreased accuracy when walking at slow speeds suggests an advantage for interlimb coordination at normal walking speeds, in contrast to previous research on dual-task walking and reach-to-grasp movements. Phasic modulations of reach precision within the step-cycle also suggest that the upper limbs are affected by the ballistic demands of motor-preparation during natural locomotion. Together these results show that the natural phases of human locomotion impose constraints on sensorimotor function and demonstrate the value of examining dynamic and natural behaviour in contrast to the traditional and static methods of psychological science.
Transcriptomic and metabolomic analyses reveal the flavor of bitterness in the tip shoots of Bambusa oldhamii Munro
Authors: Jiao Yulian
Published: September 2023

The young bamboo shoot of Bambusa oldhamii (green bamboo) has a good taste and is rich in nutrition and widely used in traditional Chinese cuisines. But the shoots flavor of Bambusa oldhamii changed from deliciously sweet to a little bitter when the shoots grew from underground to aboveground. In this paper, we explored the bitterness chemicals of the green bamboo shoot when growing from underground to aboveground using transcriptome and metabolome techniques. Finally, several bitter chemicals were mined out counting for the flavor transformation, such as Solanidine, Amygdalin, Salicin, Arbutin, and others. The transcription factor family of AP2/ERF plays the main role in key bitter chemical regulation via correlation analysis. Moreover, the pathway of Biosynthesis of phenylpropanoids might be the key pathway in the formation of the bitter chemicals in green bamboo shoot development.
Discrepancies in indel software resolution with somatic CRISPR/Cas9 tumorigenesis models
Authors: Brockman Qierra R. , Scherer Amanda , McGivney Gavin R. , Gutierrez Wade R. , Rytlewski Jeffrey , Sheehan Alexa , Warrier Akshaya , Laverty Emily A. , Roughton Grace , Carnevale Nina C. , Knepper-Adrian Vickie , Dodd Rebecca D.
Published: September 2023

CRISPR/Cas9 gene editing has evolved from a simple laboratory tool to a powerful method of in vivo genomic engineering. As the applications of CRISPR/Cas9 technology have grown, the need to characterize the breadth and depth of indels generated by editing has expanded. Traditionally, investigators use one of several publicly-available platforms to determine CRISPR/Cas9-induced indels in an edited sample. However, to our knowledge, there has not been a cross-platform comparison of available indel analysis software in samples generated from somatic in vivo mouse models. Our group has pioneered using CRISPR/Cas9 to generate somatic primary mouse models of malignant peripheral nerve sheath tumors (MPNSTs) through genetic editing of Nf1. Here, we used sequencing data from the in vivo editing of the Nf1 gene in our CRISPR/Cas9 tumorigenesis model to directly compare results across four different software platforms. By analyzing the same genetic target across a wide panel of cell lines with the same sequence file, we are able to draw systematic conclusions about the differences in these software programs for analysis of in vivo-generated indels. Surprisingly, we report high variability in the reported number, size, and frequency of indels across each software platform. These data highlight the importance of selecting indel analysis platforms specific to the context that the gene editing approach is being applied. Taken together, this analysis shows that different software platforms can report widely divergent indel data from the same sample, particularly if larger indels are present, which are common in somatic, in vivo CRISPR/Cas9 tumor models.
Predictors of lower exercise capacity in patients with cancer
Authors: Evertz Ruben , Diehl Christine , Gödde Katharina , Valentova Miroslava , Garfias-Veitl Tania , Overbeck Tobias R. , Braulke Friederike , Lena Alessia , Hadzibegovic Sara , Bleckmann Annalen , Keller Ulrich , Landmesser Ulf , König Alexander O. , Hasenfuss Gerd , Schuster Andreas , Anker Markus S. , von Haehling Stephan
Published: September 2023

Maintaining cancer patients’ exercise capacity and therefore patients’ ability to live a self-determined life is of huge importance, but little is known about major determinants. We sought to identify determinants of exercise capacity in patients with a broad spectrum of cancer types, who were already receiving cancer treatment or about to commence such therapy. Exercise capacity was assessed in 253 consecutive patients mostly suffering from advanced cancer using the 6-min walk test (6-MWT). All patients underwent echocardiography, physical examination, resting electrocardiogram, hand grip strength (HGS) measurement, and laboratory assessments. Patients were divided into two groups according to the median distance in the 6-MWT (459 m). Patients with lower exercise capacity were older, had significantly lower HGS and haemoglobin and higher values of high sensitive (hs) Troponin T and NT-proBNP (all p < 0.05). Whilst the co-morbidity burden was significantly higher in this group, no differences were detected for sex, body mass index, tumor type, or cachexia (all p > 0.2). Using multivariable logistic regression, we found that the presence of anaemia (odds ratio (OR) 6.172, 95% confidence interval (CI) 1.401–27.201, p = 0.016) as well as an increase in hs Troponin T (OR 3.077, 95% CI 1.202–5.301, p = 0.019) remained independent predictors of impaired exercise capacity. Increasing HGS was associated with a reduced risk of a lower exercise capacity (OR 0.896, 95% CI 0.813–0.987, p = 0.026). Screening patients for elevated hs troponin levels as well as reduced HGS may help to identify patients at risk of lower exercise capacity during cancer treatment.
Numerical treatment and global error estimation for thermal electro-osmosis effect on non-Newtonian nanofluid flow with time periodic variations
Authors: Ahmed O. S. , Eldabe N. T. , Abou-zeid M. Y. , El-kalaawy O. H. , Moawad S. M.
Published: September 2023

The essential purpose of this study is to discuss the impact of time-periodic variations on mixed convection heat transfer for MHD Eyring-Powell nanofluid. The fluid flows through a non-Darcy porous medium over an infinite vertical plate. The effects of viscous dissipation, Ohmic dissipation, electro-osmosis force, heat source, thermal radiation, Dufour feature, and chemical reaction are presumed. The system of partial differential equations which governs the problem is transformed into a system of non-linear algebraic equations and then an explicit finite difference approach is espoused to solve these nonlinear algebraic equations. The numerical results for the velocity, temperature, and nanoparticles concentration distributions are computed and displayed through a set of graphs. Also, the skin friction coefficient, reduced Nusselt number, and Sherwood number are computed numerically for various values of the physical parameters. It is found that the velocity becomes greater with an elevation in the value of the Helmholtz–Smoluchowski velocity. Meanwhile, it enlarges with rising in the value of the electro-osmotic parameter. The rise in the value of the thermal radiation parameter causes a dwindling influence on both temperature and nanoparticles concentration. Investigations of these effects together are very useful due to their important vital applications in various scientific fields, especially in medicine and medical industries, such as endoscopes, respirators, and diverse medical implementations, as nanoparticles can be utilized in the remedy of cancer tumors. Additionally, electroosmotic flow is important due to its ability to control fluid movement and enhance mass transport, making it valuable in various application such as sample separation, drug delivery, and DNA analysis, offering enhanced efficiency and sensitivity.
Bubble-enhanced basanite–tephrite mixing in the early stages of the Cumbre Vieja 2021 eruption, La Palma, Canary Islands
Authors: González-García Diego , Boulesteix Thomas , Klügel Andreas , Holtz François
Published: September 2023

Syneruptive magma mixing is widespread in volcanic eruptions, affecting explosivity and composition of products, but its evidence in basaltic systems is usually cryptic. Here we report direct evidence of mixing between basanitic and tephritic magmas in the first days of the 2021 Tajogaite eruption of Cumbre Vieja, La Palma. Groundmass glass in tephritic tephra from the fifth day of the eruption is locally inhomogeneous, showing micron-scale filamentary structures of Si-poor and Fe-, Mg-rich melt, forming complex filaments attached to bubbles. Their compositional distribution attests the presence of primitive basanitic magma, with compositions similar to late-erupted melts, interacting with an evolved tephritic melt during the first week of the event. From filament morphology, we suggest their generation by dragging and folding of basanitic melt during bubble migration through melt interfaces. Semi-quantitative diffusion modelling indicates that the filamentary structures are short-lived, dissipating in timescales of tens of seconds. In combination with thermobarometric constraints, we suggest a mixing onset by sub-Moho remobilization of a tephritic reservoir by basanite input, followed by turbulent ascent of a mingled magma. In the shallow conduit or lava fountain, bubble nucleation and migration triggered further mingling of the distinct melt-phases. This phenomenon might have enhanced the explosive behaviour of the eruption in such period, where violent strombolian explosions were common.
Optical phase retrieving of a projected object by employing a differentiation of a single pattern of two-beam interference
Authors: Ramadan W. A. , El-Tawargy A. S. , Wahba H. H.
Published: September 2023

In this work, we present a new approach to retrieve the optical phase map of an object which is projected by a single differentiated two-beam interference pattern. This approach is based on the differentiation of the intensity equation of the two-beam interference with respect to the carrier’s phase angle. Therefore, two interference patterns which are shifted by a very small phase angle can be obtained. Then, these two patterns are projected on the object. By exploiting the definition of the mathematical differentiation, the optical phase object’s variations are retrieved from the recorded intensity distributions of both projected patterns. According to this method, the extracted optical phase angles are raised as an inverse “sin” function. This means that the unwrapping process of this function limits the recovered phase angles between − π/2 and π/2. So, the unwrapping process of these unusual wrapped phase angles is explained. The proposed method is applied on (a) two objects which are simulated by combinations of multiple Gaussian functions and (b) a 3D real object. It is found that the inclination of the projected interference pattern on the object redistributes the intensity distribution due to the Lamber’s “cos” aw of illumination. This effect is considered in the retrieving process of the object’s phase map. The limitations of the presented method are discussed and the obtained results are found promising.