bluuebunny/crossref_metadata_2025 huggingface dataset:
Linear Matrix Inequalities in Fault Detection Filter Design for Linear Ostensible Metzler Systems
Authors: Krokavec Dušan , Filasová Anna
Published: January 2025

The article deals with the properties of fault detection filters when applying their structure to a class of linear, continuous-time systems, with dynamics being specified by the system matrix of the ostensible Metzler structure. The proposed solution is reduced to the use of diagonal stabilization in the synthesis of the state observer and uses the decomposition of the ostensible Metzler matrix. The approach creates a unified framework that covers the compactness of parametric constraints on Metzler matrices and their quadratic stability. Due to the complexity of such constraints, the design conditions are formulated using sharp linear matrix inequalities. For potential application in network control structures, the problem is formulated and solved for linear discrete-time ostensible positive systems. Finally, a linearized model of the B747-100/200 aircraft is used to validate the proposed method. The numerical solution and simulation results show that the proposed approach provides superior sensitivity of the fault detection filter in detecting faults, compared to synthesis methods that do not guarantee the positivity of the filter gain.
Ethyl Acetate Extract of Cichorium glandulosum Activates the P21/Nrf2/HO-1 Pathway to Alleviate Oxidative Stress in a Mouse Model of Alcoholic Liver Disease
Authors: Qi Shuwen , Zhang Chunzi , Yan Junlin , Ma Xiaoyan , Zhong Yewei , Hou Wenhui , Zhang Juan , Pang Tuxia , Ma Xiaoli
Published: January 2025

Background: Alcoholic liver disease (ALD) is a significant global health concern, primarily resulting from chronic alcohol consumption, with oxidative stress as a key driver. The ethyl acetate extract of Cichorium glandulosum (CGE) exhibits antioxidant and hepatoprotective properties, but its detailed mechanism of action against ALD remains unclear. This study investigates the effects and mechanisms of CGE in alleviating alcohol-induced oxidative stress and liver injury. Methods: Ultra-Performance Liquid Chromatography coupled with Quadrupole-Orbitrap Mass Spectrometry (UPLC-Q-Orbitrap-MS) was used to identify CGE components. A C57BL/6J mouse model of ALD was established via daily oral ethanol (56%) for six weeks, with CGE treatment at low (100 mg/kg) and high doses (200 mg/kg). Silibinin (100 mg/kg) served as a positive control. Liver function markers, oxidative stress indicators, and inflammatory markers were assessed. Transcriptomic and network pharmacology analyses identified key genes and pathways, validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Results: UPLC-Q-Orbitrap-MS identified 81 CGE compounds, mainly including terpenoids, flavonoids, and phenylpropanoids. CGE significantly ameliorated liver injury by reducing alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels and enhancing antioxidative markers such as total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) while lowering hepatic malondialdehyde (MDA) levels. Inflammation was mitigated through reduced levels of Tumor Necrosis Factor Alpha (TNF-α), Interleukin-1 Beta (IL-1β), and C-X-C Motif Chemokine Ligand 10 (CXCL-10). Transcriptomic and network pharmacology analysis revealed seven key antioxidant-related genes, including HMOX1, RSAD2, BCL6, CDKN1A, THBD, SLC2A4, and TGFβ3, validated by RT-qPCR. CGE activated the P21/Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2)/Heme Oxygenase-1 (HO-1) signaling axis, increasing P21, Nrf2, and HO-1 protein levels while suppressing Kelch-like ECH-associated Protein 1 (Keap1) expression. Conclusions: CGE mitigates oxidative stress and liver injury by activating the P21/Nrf2/HO-1 pathway and regulating antioxidant genes. Its hepatoprotective effects and multi-target mechanisms highlight CGE’s potential as a promising therapeutic candidate for ALD treatment.
Prevalence of Hepatitis C Virus Among Hospitalized Patients in a Tertiary Hospital in Italy: The Basis for a National Screening Assessment Model?
Authors: Morsica Giulia , Locatelli Massimo , Hernandez-Ibarburu Gema , Rusconi Francesca , Segovia-Hilara Alba , Di Napoli Davide , Moro Matteo , Mazzitelli Salvatore , Hasson Hamid , Esposti Federico , Mazzuconi Roberts , Castagna Antonella
Published: January 2025

Free-of-charge hepatitis C virus antibody (HCV Ab) screening in some key populations and in 1969–1989 birth cohorts have been funded in Italy as the first step in confirming diagnosis in individuals who may be unaware of their infection. The purpose of this study is to leverage existing in-hospital routine screening data to better understand the distribution of HCV. A retrospective study of hospitalized patients (PTs) tested for HCV Ab for 5 years (from January 2017 to December 2022) in San Raffaele hospital was conducted according to age categories: birth year group before 1947 (patients older than 76 years old), birth year group 1947–1968, birth year group 1969–1989, and two other groups with birth year groups 1990–2000 and 2001–2022 (with patients younger than 33 years old) using the TriNetX platform. Among the 42,805 in-hospital PTs tested, 1297 (3.03%) were HCV Ab positive. The prevalence of HCV Ab was greater in PTs over the age of 76 (5.3%), whereas it was lower in the youngest birth year cohort (2000–2022, 0.16%). Among 1297 HCV Ab positive PTs, only 198 (15.3%) were tested for the presence of HCV RNA. The birth cohort 1969–1989 had a modest seroprevalence (1.5%), yet they were the most affected age group, with 44.4% being HCV RNA positive. The in-hospital HCV screening including birth year cohort 1947–1989 could be a more valuable option compared to the screening for birth year group 1969–1989 in the general population.
Impact of Caffeine Intake on Female Basketball Players’ Performance
Authors: Nieto-Acevedo Raúl , García-Sánchez Carlos , Bravo-Sánchez Alfredo , Abián-Vicén Javier , Abián Pablo , Portillo Javier , Martínez-Rubio Carlos , Lorenzo Calvo Jorge , Diaz-Lara Javier
Published: January 2025

Background: This study aimed to analyze the effect of caffeine ingestion on basketball performance in semi-professional female players. Methods: A double-blind, placebo-controlled, randomized experimental design was conducted, in two different periods separated by a week. Twelve female basketball players ingested 3 mg of caffeine/kg of body mass or a placebo. After 60 min, participants completed two repetitions of the specified sequence: Abalakov jump, countermovement jump (CMJ), 5-0-5 (505) test, and 20 m sprint. Later, external loads and game statistics were recorded during a 30-min simulated basketball game. Results: Significant improvements were observed in CMJ height and 505 test with caffeine ingestion compared to the placebo. Significant moderate differences were evident between conditions in external load variables, specifically, an increased number of high-intensity changes of direction during simulated games with caffeine ingestion. Two-point shot efficiency significantly improved after pre-caffeine ingestion compared with placebo. Conclusions: Three mg of caffeine per kg of body mass may serve as an effective ergogenic aid to increase physical performance and some variables of performance index in highly trained basketball players.
Plasma Lipid Profile Among Perimenopausal Latvian Women in Relation to Dietary Habits
Authors: Aleksejeva Svetlana , Meija Laila , Zolovs Maksims , Ciprovica Inga
Published: January 2025

Background/Objectives: Hormonal changes throughout a woman’s life cycle significantly affect serum lipid levels. Alterations in the serum lipid profile can increase the risk of cardiovascular diseases (CVDs). Additionally, nutrition and dietary habits are crucial for managing dyslipidemia. The current study evaluated the association between dietary habits and plasma lipid profiles among perimenopausal women in Latvia. Methods: The randomized clinical trial involved perimenopausal women (n = 61) aged 49 ± 3 years with moderately high low-density lipoprotein cholesterol (LDL-C) levels of 3.61 ± 0.30 mmol L−1. A series of questionnaires were completed, including a questionnaire on the subject’s demographic, anthropometric, lifestyle, health, physical activity, and dietary factors, a 24 h food diary, a 72 h food diary, and a food-frequency questionnaire (FFQ). Blood testing was conducted for all participants, which included total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), alanine aminotransferase (ALAT), and glucose level analyses. Results: The consumption of refined sugar, honey, syrup, and jam demonstrated a strong positive association with higher levels of remnant cholesterol (β = 0.462, p ≤ 0.05) and non-high-density lipoprotein cholesterol (non-HDL-C) (β = 0.395, p ≤ 0.05). Similarly, the consumption of fruit juices is associated with increased LDL-C (β = 0.303, p ≤ 0.05) and non-HDL-C (β = 0.285, p ≤ 0.05). Conversely, higher meat and poultry consumption negatively correlates with TC levels (β = −0.290, p ≤ 0.05). Conclusions: This underscores the need for further examination to understand the impact of dietary habits on lipid profile.
Infectious Laryngotracheitis Virus and Avian Metapneumovirus: A Comprehensive Review
Authors: Mo Jongsuk , Mo Jongseo
Published: January 2025

Respiratory avian viral diseases significantly impact the world poultry sector, leading to notable economic losses. The highly contagious DNA virus, infectious laryngotracheitis virus, and the RNA virus, avian metapneumovirus, are well known for their prevalent effects on avian respiratory systems. The infectious laryngotracheitis virus (ILTV), stemming from the Herpesviridae family, manifests as an upper respiratory disease within birds. Characterized by acute respiratory signs, it sporadically emerges worldwide, presenting a persistent threat to poultry health. Avian metapneumovirus (aMPV), belonging to the Pneumoviridae family is identified as the cause behind severe rhinotracheitis in turkeys and swollen head syndrome in chickens. This disease can lead to heightened mortality rates, especially when coupled with secondary bacterial infections. This review offers a comprehensive analysis and understanding of the general properties of these specific avian respiratory viruses, control measures, and their global status.
Dimensional Accuracy of Polyether Elastomeric Impression Materials After Using Chitosan as a Disinfectant: A Sustainable Approach to Dental Infection Control
Authors: Taymour Noha , Alabdrubalameer Dooa I. , Al Shooq Zahra H. , Al Yaseen Maram S. , Al Zaher Rabab H. , Shetty Ashwin C. , Rifaat Shimaa
Published: January 2025

Background: With the growing demand for sustainable dental practices, chitosan has evolved as an eco-friendly disinfectant for polyether impressions. Objectives: The present study investigated the disinfectant efficacy of chitosan on the dimensional accuracy of polyether (PE) impression material. Methods: A total of 63 polyether impressions (ImpregumTM, PentaTM, 3M ESPE, Boca Raton, FL, USA) were prepared from the master 3D-printed model, each consisting of a single unit abutment facing two units’ abutments. Three groups of these subjects were used, while each group comprised 21 impressions, respectively. Group 1 is used as a control group and is not subjected to chemical disinfection. Group 2 is spraying group, in which the PE impressions were sprayed with 0.5% chitosan with a high molecular weight. Group 3 is the immersion group, in which the impression was immersed for 15 min in 0.5% chitosan with a high molecular weight. The data collected were analyzed using SPSS 28.0 and the difference in dimensional accuracy between the groups was measured using a one-way ANOVA. Results: Both the intra-and inter-abutment measurements (MD and OG) showed no statistically significant differences in the dimensional changes between the control and the study groups, while the cross-arch distance showed a statistically significant difference in the dimensional change between control and immersion group, p = 0.000. Conclusions: While chitosan disinfection induced slight dimensional changes in polyether impressions, these alterations remained within clinically acceptable limits. The spray application method appeared to be preferable to immersion, as it resulted in less pronounced dimensional changes.
Bridging Cultural Capital: Youth-Driven Communication as a Catalyst for Well-Being in Film Festival Participation
Authors: Puccia Angelo , Cabeza-Ramírez L. Javier , de los Santos Manuel Márquez , González-Mohíno Miguel
Published: January 2025

Film festivals often struggle to engage younger audiences, posing challenges for cultural participation and well-being. This study examines an innovative communication strategy introduced by the Ibero-American Film Festival of Huelva (FCIH), which incorporates young communicators (JCC) to enhance traditional outreach efforts. By employing a qualitative case study approach, including eight semi-structured interviews and an analysis of social media activity, this research investigated how this initiative influences the transmission and acquisition of cultural capital across generations. The findings reveal a substantial growth in social media engagement (12,155%) and increased attendance among younger audiences, highlighting the successful transfer of embodied cultural capital. Grounded in Bourdieu’s theoretical framework, the JCC initiative emerges as a tool for redistributing cultural capital. This strategy not only strengthens the festival’s outreach efforts but also has the potential to transform the cultural landscape of film festivals. The study concludes that involving young voices in the communication of cultural events can effectively democratize cultural capital and bridge generational divides, extending Bourdieu’s insights into the dynamics of contemporary digital culture.
Danger Is a Signal, Not a State: Bigaagarri—An Indigenous Protocol for Dancing Around Threats to Wellbeing
Authors: Orcher Phillip , Palmer Victoria J. , Yunkaporta Tyson
Published: January 2025

This paper describes the health and wellbeing applications of a protocol designed from a Gumbaynggirr Australian First People’s concept, Bigaagarri. The protocol reframes threats to health and wellbeing as part of a communicative system of environmental signals, rather than an individualised, behavioural fight–flight–fear response. Developed by a Muruwari Gumbaynggirr researcher, the protocol enfolds Aboriginal perspectives of health values and the physicality of personal location in place and social context. It combines Indigenous standpoint theory and lived-experience narrative research methods to translate Indigenous practices into generally accessible modalities. The paper connects the first principles of this protocol to literature, then, using code-switching between academic and informal settler and Indigenous voices, it introduces personal lived experience narratives that include utilisation of the participatory and immersive protocol seen in the graphical abstract image to mitigate suicidal ideation. This approach unsettles Westernised conceptions of health and wellbeing research that privilege disease-specific, single-solution approaches. It contests the dominant social imaginaries and narratives embedded in standard service models, which perpetuate the ongoing recolonisation of Indigenous identities, and common exclusion of others outside of the neurotypical majority. The Bigaagarri protocol is a potential way forward to reimagine preventive health landscapes, decolonise support for suicide and mental health through the embedding of Indigenous knowledges to lead to holistic approaches for wellbeing.
Enhancing Soil Health in Rice Cultivation: Optimized Zn Application and Crop Residue Management in Calcareous Soils
Authors: Laik Ranjan , Eltahira Elsaffory Bakry Awad , Pramanick Biswajit , Nidhi  , Singh Santosh Kumar , Es Harold van
Published: January 2025

Crop residue, a readily available biomass, is the largest source of organic matter in soil, and zinc (Zn) significantly influences microbial activity. Understanding the optimal Zn rates for enhanced biological activity in crop residue-amended soils is crucial. A study at RPCAU, Pusa, examined the combined effects of Zn applications and long-term crop residue amendments on soil biological properties in a rice–wheat cropping system. Conducted on Zn-deficient calcareous soil, the experiment used a split-plot design with four crop residue levels (0, 25, 50, and 100%) and four Zn rates (0, 2.5, 5, and 10 kg ha−1). Crop residues were incorporated each season, while Zn was applied initially in 1994 and again in 2018. The results showed significant improvements in soil organic carbon, organic C-stock, and reductions in soil bulk density. A linear–plateau regression model revealed that Zn application at 10 kg ha−1 increased soil active carbon and soil respiration by 35% and 53%, respectively, with the required crop residue levels at 73.73% and 90.28%. ACE protein increased by 9.6% with Zn application at 5 kg ha−1, with a required crop residue level of 91.06%. The highest values of soil available nutrients and grain yield of rice were observed with 100% residue incorporation and 10 kg ha−1 Zn application. Thus, applying 10 kg ha−1 Zn along with 100% crop residue incorporation significantly improves soil biological properties and soil organic carbon levels in calcareous soil under a rice–wheat cropping system.
Assessment of Water Hydrochemical Parameters Using Machine Learning Tools
Authors: Malashin Ivan , Nelyub Vladimir , Borodulin Aleksei , Gantimurov Andrei , Tynchenko Vadim
Published: January 2025

Access to clean water is a fundamental human need, yet millions of people worldwide still lack access to safe drinking water. Traditional water quality assessments, though reliable, are typically time-consuming and resource-intensive. This study investigates the application of machine learning (ML) techniques for analyzing river water quality in the Barnaul area, located on the Ob River in the Altai Krai. The research particularly highlights the use of the Water Quality Index (WQI) as a key factor in feature engineering. WQI, calculated using the Horton model, integrates nine hydrochemical parameters: pH, hardness, solids, chloramines, sulfate, conductivity, organic carbon, trihalomethanes, and turbidity. The primary objective was to demonstrate the contribution of WQI in enhancing predictive performance for water quality analysis. A dataset of 2465 records was analyzed, with missing values for parameters (pH, sulfate, and trihalomethanes) addressed using predictive imputation via neural network (NN) architectures optimized with genetic algorithms (GAs). Models trained without WQI achieved moderate predictive accuracy, but incorporating WQI as a feature dramatically improved performance across all tasks. For the trihalomethanes model, the R2 score increased from 0.68 (without WQI) to 0.86 (with WQI). Similarly, for pH, the R2 improved from 0.35 to 0.74, and for sulfate, from 0.27 to 0.69 after including WQI in the feature set.
Diversification of Rural Development in Poland: Considerations in the Context of Sustainable Development
Authors: Bartkowiak-Bakun Natalia
Published: January 2025

A change in the understanding of rural policy took place in the 1980s when there was a transition from a sectoral to a territorial perception of rural areas. Rural areas are no longer identified only with agriculture. There has been a recent decentralization of rural policy to the regional and local levels, which requires the recognition of the diversity of rural areas and the changes taking place in them. Therefore, it is important to recognize the state of sustainable development and disparities at the local level so that the targeted support is appropriate. The aim of the research is to measure sustainable development, including spatial diversification. The measurement of sustainable development was carried out using synthetical measures, and measures for economic, social, and environmental domains were constructed. The Jenks method was applied to group entities into classes characterized by similar levels of development. The results of the research proved the significance of the differences in the scope of balanced development for each of the domains. The resulting spatial systems are characterized by the line of the center–periphery. The obtained research results are valuable for central and local authorities in the process of planning local and regional development.
The Esthetic Use of Botulinum Toxins in Cancer Patients: Providing a Foundation for Future Indications
Authors: Papagni Marco , Renga Monica , Mogavero Selene , Veronesi Paolo , Cavallini Maurizio
Published: January 2025

Advances in oncological treatments have improved the survival rates of cancer patients but have often resulted in significant physical changes that negatively impact their self-esteem and psychological well-being. Cancer patients frequently ask esthetic practitioners to perform procedures to address such changes. However, practitioners often hesitate to satisfy such requests due to lacking guidelines or recommendations. The use of botulinum toxins (BoNTs) for esthetic purposes has shown significant promise in improving the quality of life for cancer patients. This review explores the broad application of BoNTs in many medical branches, focusing on oncology. A substantial amount of literature shows that BoNTs are safe and effective as a type of adjunctive therapy compared to classical cancer treatments. We provide our expert opinion that the use of BoNTs for esthetic purposes is safe for cancer patients and even recommended for those whose mood is influenced by the worsening of their physical appearance. Careful patient selection and interdisciplinary collaboration are essential to the safe integration of BoNTs into cancer care.
Use, Potential, Needs, and Limits of AI in Wastewater Treatment Applications
Authors: Capodaglio Andrea G. , Callegari Arianna
Published: January 2025

Artificial intelligence (AI) uses highly powerful computers to mimic human intelligent behavior; it is a major research hotspot in science and technology, with an increasing number of applications to a wider range of fields, including complex process supervision and control. Wastewater treatment is an example of a complex process involving many uncertainties and external factors to achieve a final product with specific requisites (effluents with prescribed quality). Reducing process energy consumption, greenhouse gas emissions, and resources recovery are additional requirements of these facilities’ operation. AI could extend the purpose and the expected results of previously adopted tools and present operational approaches by leveraging superior simulation, prediction, control, and adaptation capabilities. This paper reviews current AI research in the wastewater field and discusses present achievements and potentials. So far, almost all applications in the sector involve predictive studies, often at a small scale or with limited data use. Frontline research aimed at the creation of AI-supported digital twins of real systems is being conducted, with few encouraging but still limited applications. This paper aims at identifying and discussing key barriers to wider AI adoption in the field, which include laborious instrumentation maintenance, lack of process expertise in the design of current software, instability of control loops, and insufficient incentives for resource efficiency achievement.
Legal and Safety Aspects of the Application of Automated and Autonomous Vehicles in the Republic of Croatia
Authors: Milenković Melita , Sumpor Davor , Tokić Sandro
Published: January 2025

In its draft proposal for the Road Transport Act, the Croatian government referred to European Union Directive 2022/738, which concerns the use of hired vehicles for goods transport, rather than the pertinent European Union regulations on automated and autonomous vehicles, specifically Regulation 2019/2144 and Implementing Regulation 2022/1426. This oversight highlights Croatia’s lack of preparedness to integrate highly automated and autonomous vehicles, which are crucial for safety and environmental performance as per European Union standards. This paper aims to clarify the safety and legal recommendations for the trafficking of these vehicles in Croatia. Level 2 and Level 3 automated vehicles, present in smaller numbers in road traffic in Croatia, were compared from the perspective of the lack of driving tasks and its impact on driver safety. The stages of road liability for traffic accidents were also investigated, with recommendations of strict (default) liability of manufacturers for fully autonomous vehicles as well as presumed liability of all road traffic participants for highly automated vehicles. The safety and traffic benefits of possible infrastructure upgrades for highly automated and fully autonomous vehicles were discussed, mostly in the segment of dedicated lines.
Enhancing geometry learning through project-based teaching materials: A focus on circumference and area of circles
Authors: Sari Lili Nur Indah , Hoiriyah Diyah
Published: January 2025

This study aims to develop a Student Worksheet based on the Project-Based Learning (PjBL) approach, ensuring its validity, practicality, and effectiveness in facilitating mathematics instruction on the topic of circumference and area of circles for MTs students. The research follows a Research and Development (R&D) methodology, employing the ADDIE development model, which encompasses five stages: Analysis, Design, Development, Implementation, and Evaluation. The validation process assessed the worksheet’s quality across four aspects: content feasibility, material presentation, worksheet structure, and linguistic quality. The results indicate that the content feasibility scored 84%, material presentation 86.5%, worksheet elements 86%, and linguistic quality 88%, all within the “very valid” category. Overall, the Student Worksheet achieved a validity score of 86% (categorized as “very valid”), confirming its appropriateness for use in the classroom. These findings suggest that the PjBL-based Student Worksheet effectively supports mathematics learning and meets expert criteria for validity.
The teaching-learning process of the continuity of real variable functions: A literature review
Authors: Maxil-Cardoso G. Elizabeth , Castro-Fernández Felipe , Juárez-Lopez José Antonio
Published: January 2025

This article´s objective is to present the current state of research on the teaching-learning process of continuity of real variable functions. To achieve this, a documentary research design with a qualitative approach and a descriptive depth level was adopted. The instruments for data collection and analysis included a search log, a bibliographic matrix, and a synthesis matrix, created using Excel software. The technique used for the literature review was qualitative content analysis, based on deductive analytical categories: objective, theoretical framework, research method, and conclusions. Advanced searches were conducted on Google Scholar, on Scopus and Web of Science and out of the total results found, 13 relevant articles published during the period 2020-2023 were selected. Among the main findings, the use of a variety of theoretical frameworks in the foundation of the analysed studies was identified, such as the theory of semiotic representations, the MTSK model, the TPACK, and the conceptual contributions of Tall and Vinner, among others. Additionally, a growing interest in the use of technological tools, such as GeoGebra, to enhance the teaching and learning process of mathematics in general, and the continuity functions in particular. Although its limitations, it was identified that this software can be used as a tool for visualization, construction, representation and communication of mathematical knowledge.
Going down the rabbit hole: insight into the future of Paramecium (Ciliophora, Protista) biodiversity surveys
Authors: Tarcz Sebastian
Published: January 2025

Paramecium, a research subject in many areas of life sciences, appeared to be a ciliate genus with a well known biodiversity structure. However, the understanding of its biological diversity has been evolving rapidly in recent years, driven by the discovery of new taxa and an
expanded knowledge of the distribution of known species. Most future insights into Paramecium biodiversity are expected to come from molecular data, particularly through eDNA sampling. As one of the most recognisable microeukaryotes, commonly found in freshwater ecosystems, and with
over a century of biodiversity research – including extensive reference data from GenBank records and living culture collections – Paramecium holds significant potential to become a model ciliate for studies in biodiversity and biogeography. This review addresses the challenges
of species identification within the Paramecium genus, the current state of knowledge on its biodiversity and other factors that may shape future research. Despite some existing bottlenecks, new approaches to data acquisition and analysis will enable researchers to integrate diverse
lines of evidence, allowing for exceptional explorations of Paramecium species and populations.
Novel cytogenetic data in two gudgeons from the genera Gobio and Romanogobio (Teleostei: Cypriniformes: Gobionidae): chromosomal mapping of rDNAs and telomeric repeats
Authors: Kirtiklis Lech , Ocalewicz Konrad , Nowak Michał , Gwiazda-Orzoł Marta , Boroń Alicja
Published: January 2025

The taxonomic status of gudgeons (Gobionidae) and the genetic relationships among them are still unclear. The current knowledge on the molecular cytogenetics of gudgeons is quite limited and concerns the basic parameters of their karyotype. Therefore, the main aim of this study was
to obtain new data on the chromo- somes of two gudgeons – Gobio gobio and Romanogobio belingi – which would allow for characterising these species in a more precise way. Chromosomes of the examined species were analysed by conventional Giemsa staining and, for the
first time, by fluorescence in situ hybridisation (FISH) with 28S rDNA, 5S rDNA and telomeric probes. The diploid chromosome numbers in both species were the same and equalled 2n=50, while the chromosome arm number (NF) was 96 and 98 in G. gobio and R. belingi, respectively.
Moreover, small interspecific differences concerning the chromosomal morphology were observed. Major 28S rDNA sites were mapped to the short (p) arms of two submetacentric chromosomes with a similar size in G. gobio, whereas in R. belingi, FISH signals were observed from
the short and the long (q) arms of two different-sized submetacentric chromosomes. Minor 5S rDNA sites were found on the p arms of eight submetacentric chromosomes of different sizes in G. gobio and on five submetacentric chromosomes in R. belingi. Unique chromosomal
co-locations of major and minor rDNA sites were observed in the karyotypes of both species. In the chromosomes of both species, telomeric DNA sequences were typically located at the ends of all chromosomes. The number of chromosome arms, as well as the number and lo- cation of 45S and 5S rDNAs,
and the number of unexpected co-located major and minor ribosomal genes may be useful cytotaxonomic species-specific markers.
Synthesis, hardness and tensile behavior of boron carbide reinforced Al6082 alloy composites for automotive applications
Authors: Namdev Nagaraj , Kukanur Vijayendra , Bilgundi Shivkumar , Bandi Swetha Kuntebomanahalli , Vishwanath Katagondanahalli Chikkanna , Kumar Subbaraya Mohan , Valukula Balaraj , Manjunatha T. H.
Published: January 2025

The present study aims to synthesise Al6082 alloy including 4 wt. % of tiny B4C particulate in metal matrix composites by liquid metallurgical techniques. Scanning electron microscopy pictures of the synthesised composites were acquired to illustrate the homogeneous distribution of the reinforced micro B4C particles within the matrix. Hardness, tensile, and impact tests were conducted to assess the influence of micro B4C on the mechanical properties of the composites. The hardness, tensile strength, and impact strength of synthesised composites increased with the addition of B4C particles.
Incidência de burnout em profissionais da saúde: uma revisão da literatura
Authors: Jung Luana , Guedes Mariana Resende , Santos Ana Paula Resende Passos , Moraes Monalysa Rodrigues Borges , Barcelos Raul Vidica Teodoro , Guedes Natália Resende , Guedes Laerte Leite
Published: January 2025

Objetivo: Avaliar a incidência e os principais fatores de risco para o burnout em profissionais de saúde. Metodologia: Corresponde a uma revisão sistemática da literatura existente nas bases de dados científicas MEDLINE, EMBASE e Biblioteca Virtual em Saúde, mediante uma abordagem qualitativa, realizada durante o mês de novembro de 2024, através de um processo de busca direcionada utilizando combinações de descritores DeCS/MeSH com a inclusão do operador “and” entre os termos, de acordo com o representado: "Burnout" and "Health Care Professional" e "Burnout" and "Profissionais de Saúde". Resultados e Discussão: Após o processo de pesquisa dos estudos nas bases de dados elencadas, de acordo com os filtros utilizados, foram selecionados 14 estudos para participarem do embasamento teórico desta pesquisa científica. Esses estudos foram escolhidos de acordo com critérios de exclusão e inclusão, além de serem submetidos, posteriormente, por uma etapa de avaliação e sistematização dos dados, visando definir a incidência de burnout em profissionais que trabalham na área da saúde. Considerações Finais: Os profissionais de saúde enfrentam altos índices de exaustão mental, caracterizada pelo burnout, principalmente se trabalham em UTI, em emergência e em cuidados paliativos, devido a atuação com pacientes críticos, demanda excessiva e desvalorização profissional.
Qualidade de vida de pessoas que convivem com a colostomização atendidas pelo Sistema Único de Saúde em Blumenau, SC
Authors: Schelbauer Mariana Carolina Câmara , Santos Alessandra Cunha dos , Souza Daniela Maysa de
Published: January 2025

As ostomias são procedimentos cirúrgicos realizados para exteriorizar órgãos, podendo ser temporárias ou permanentes, sendo que a colostomia consiste na exteriorização de qualquer segmento do cólon. A convivência com a bolsa traz alterações físicas e psicossociais. Objetiva-se com esse estudo, identificar o nível da qualidade de vida dos pacientes colostomizados atendidos no Núcleo de Atenção ao Ostomizado (NAO) no município de Blumenau-SC. A pesquisa é de cunho quantitativo-descritivo e foi realizada com os pacientes que frequentam os serviços do NAO, por meio do questionário Stoma Care QoL, composto por 20 perguntas que avaliam a qualidade de vida desses pacientes. O estudo incluiu 47 pessoas colostomizadas, sendo a maioria mulheres, com faixa etária predominante entre 60 e 74 anos, e a maior parte usava a bolsa há menos de cinco anos. A principal causa para a realização da colostomia foram condições agudas. As preocupações mais relatadas foram odor, ruído e vazamentos da bolsa. Em relação à qualidade de vida, a dimensão pessoal foi a mais afetada, sem diferenças significativas entre os gêneros. Pacientes com 75 anos ou mais apresentaram uma qualidade de vida inferior, enquanto o motivo da cirurgia não influenciou nos escores de qualidade de vida. Observou-se também que, quanto menor o tempo de uso da bolsa, piores foram os índices de qualidade de vida. Conclui-se que, muitas variáveis influenciam na qualidade de vida da pessoa colostomizada e que estratégias de cuidado multidisciplinares são necessárias para promover uma melhor qualidade de vida e um processo de adaptação mais humanizado.
Screening de retinopatia diabética em pacientes previamente diagnosticados
Authors: Nogueira Gustavo Guimarães , Fonseca Arthur Baracho da , Maia Caio Antônio de Lima , Maia Júlia Bontempo , Costa Pedro Guimarães , Botelho Daniel de Pinho , Damásio Mariana Amaranto de Souza
Published: January 2025

A Retinopatia Diabética (RD) é uma das complicações mais comuns e graves do diabetes mellitus, sendo uma das principais causas de cegueira evitável em adultos em idade produtiva no mundo. O desenvolvimento dessa condição está intimamente associado à duração do diabetes e ao controle glicêmico inadequado, resultando em danos progressivos aos vasos sanguíneos da retina. O rastreamento precoce, ou screening, é uma estratégia essencial para a detecção oportuna da RD, permitindo intervenções precoces que podem prevenir ou retardar a progressão da doença e suas complicações visuais. Em pacientes previamente diagnosticados com diabetes, o acompanhamento regular e sistemático é particularmente crucial, dado o risco elevado de complicações oculares. Este estudo tem como objetivo quantificar pacientes assisntomáticos com Retinopatia Diabética, analizar a relação entre o uso de insulina e achados da doença, avaliar a associação entre retinopatia e outras comorbidades, e identificar pacientes que desconheciam o progresso da retinopatia. Foram avaliados 71 pacientes que já tinham sido diagnosticados com diabetes no Centro de Especialidades Médicas de Vespasiano, MG.
Insuficiência hepática aguda associada a Síndrome de DRESS: um relato de caso
Authors: Côco Luiza Zuccon , Andrade Bruno Tardin de , Hadad David Jamil
Published: January 2025

A síndrome de hipersensibilidade induzida por fármacos é uma reação induzida por medicamentos de acometimento sistêmico também conhecida como DRESS. Pode ser caracterizada pela tríade que inclui febre, erupção cutânea e acometimento de órgãos internos. A real incidência é desconhecida e não há predileção por sexo ou idade. A reação cutânea geralmente começa 2 a 8 semanas após o medicamento ser iniciado e se mantém após a sua suspensão, podendo persistir também durante várias semanas. A erupção pode recorrer em casos de readministração das medicações. Têm sido relatadas taxas de mortalidade de até 10%, com a maior parte decorrendo de insuficiência hepática. O objetivo deste relato de caso é descrever o caso clínico de uma paciente do sexo feminino, 33 anos, internada com síndrome DRESS e acometimento hepático agudo, além de outras disfunções sistêmicas graves.
Lesões ligamentares nas fraturas articulares da extremidade distal do rádio por avaliação artroscópica
Authors: Salame Jéssica Paola , Biz Maria Eduarda Zen , Figueiredo Henrique Soares de , Buchen Carlos Eduardo , Parrela Luiz Fernando Silveira
Published: January 2025

O conhecimento sobre lesões ligamentares em fraturas de rádio distal, com auxílio do uso de artroscopia, ampara o cirurgião para a realização de um diagnóstico preciso e também colabora na obtenção de maior sucesso cirúrgico devido a visualização direta das lesões. Objetiva-se analisar os prontuários de 20 pacientes com fraturas de rádio distal submetidos a osteossíntese assistida por artroscopia. Os participantes incluídos tinham entre 18 e 80 anos, apresentavam fraturas intra-articulares da extremidade distal do rádio e foram submetidos à técnica cirúrgica que empregou um acesso minimamente invasivo com fixação da fratura e subsequente artroscopia diagnóstica e terapêutica, avaliando as lesões ligamentares conforme as classificações de Geissler e Palmer. As análises estatísticas, incluindo o Teste de Mann-Whitney e Teste Exato de Fischer, exploraram a relação entre as variáveis de grupamento (Faixa Etária e Tipo de Trauma) e a severidade das lesões ligamentares (Ligamento Escafossemilunar, Ligamento Semilunopiramidal e o Complexo fibrocartilagem triangular). Os resultados estatísticos indicaram que lesões do Complexo fibrocartilagem triangular, foram mais comuns em traumas de baixa energia, principalmente entre idosos, enquanto lesões dos Ligamentos Escafossemilunar e Semilunopiramidal variaram significativamente com o tipo de trauma, porém tendem a estar associados com maior prevalência a traumas de alta energia, os quais, são mais comuns em jovens. Isto leva a concluir que a artroscopia do punho nessas fraturas apresentou-se como um importante método para diagnóstico e tratamento precoce das lesões ligamentares associadas e lesões de fibrocartilagem triangular.
Perfil clínico e epidemiológico da população infantil do Programa de Saúde Auditiva da Amazônia entre 2006 a 2023
Authors: Oliveira Camila Araújo , Rodrigues Jussandra Cardoso , Amaral Naiade de Oliveira do , Cunha Paulo André Gonçalves da , Olinto Bianca Sousa Duarte Rotoli , Siqueira Ana Alicia Amorim , Vinagre Lucas da Silva , Brito Giovana Pereira Lobato
Published: January 2025

A deficiência auditiva é uma problemática comum em nascidos vivos, mas com monitoramento e diagnósticos precoce a oportunidade de remediação se mostra promissora com o cuidado e tratamento. Assim, temos por objetivo geral de em identificar o perfil clínico e epidemiológico da população infantil atendida no programa de saúde auditiva em um hospital de referência na cidade de Belém no estado do Pará. A pesquisa de cunho quantitativo e com análise estatística utilizando o software Epi-info7, compreendeu os dados do Ambulatório de Saúde Auditiva Infantil pelo Programa de Saúde Auditiva em um hospital de referência na Amazônia, das crianças cadastradas no período de 2006 a 2023. Como resultados principais, a suspeita de perda auditiva surgiu entre menor de um ano (30,9%) até acima de oito anos de idade (9,2%), a maior proporção do primeiro atendimento entre um e três anos (58,1%). Além disso, a mais frequente queixa dos pacientes foi a Hipoacusia com 64.8%. Somado com mais frequência das consequências da perda adutiva estão as alterações da fala (78.7%) e na habilidade escolar (66.5%). Por fim, identificamos a importância de programas de intervenção precoce e multidisciplinar para minimizar os impactos da perda auditiva no desenvolvimento infantil, ressaltando o papel das unidades básicas de saúde nos encaminhamentos e a necessidade de maior acesso ao uso de aparelhos de amplificação sonora individual.
Estratégias no manejo de infecção de endoprótese aortoilíaca: revisão da literatura
Authors: Nicoletti Ana Luíza Alves , Cardoso Edson Hugo Ferreira de Lima
Published: January 2025

Introdução A infecção de endopróteses do segmento aortoilíaco após correção de aneurismas é uma complicação temida devido ao difícil manejo e da alta morbimortalidade. O entendimento da doença e das atuais estratégias de manejo facilita a condução dos casos. Metodologia Trata-se de uma revisão bibliográfica com análise de artigos já publicados a partir de busca em três bases de dados (Scielo, PubMed e Science Direct). Discussão A infecção de endoprótese é uma complicação rara e o diagnóstico se dá por meio dos critérios MAGIC. O padrão ouro para o tratamento envolve a retirada da prótese seguida por revascularização in situ ou extra anatômica. Entretanto, em casos específicos, o tratamento conservador pode ser considerado, apesar de estar relacionado com piores prognósticos. Conclusão A condução de casos de infecção de endoprótese é um desafio e, apesar do explante da prótese com revascularização ser o padrão ouro, outras estratégias podem ser consideradas em casos específicos.
Understanding Klebsiella pneumoniae carbapenem resistant profile recovered in a Brazilian university hospital
Authors: Nunes Mariana Silva , Vieira Cristina Dutra , Nobre Junior Vandack Alencar , Santos-Key Simone Gonçalves dos
Published: January 2025

Problem: Carbapenem-resistant strains of K. pneumoniae (CRKP) represent 70 to 90% of public health infections. Considering the difficulty in launching new pharmacological options, and the imminent threat of outbreaks, new studies are necessary. Objectives: To investigate antimicrobial resistance and virulence factors of CRKP strains recovered in the Clinical Hospital from the Federal University of Minas Gerais State, from October 2016 to January 2018. Methodology: Medical records of twenty-five patients were included and age ranged from 18 to 76 years old. 26 CRKP isolates were identified by MALDI-TOF. The antimicrobial susceptibility profile was determined by broth microdilution or disk-diffusion tests. The modified carbapenem inactivation method searched for carbapenemase, and the string test for hypermucoviscous phenotypes. Biofilm production was evaluated by quantitative assay, and the irp2, mrkD, fimH, and blaKPC genes were screened by conventional PCR. Results: Medical records showed previous use of antibiotics (92%), ICU hospitalization (44%), use of invasive medical devices (72%), and the presence of comorbidities (96%). Charlson comorbidity index (CCI) among patients ranged from 0-12 with an average score of 4.7. The highest score observed (12) was in a 75yo patient who was diagnosed with chronic kidney disease, and other comorbidities. The prevalence of blaKPC among the CRKP strains was 92%. Conclusions: Although no strains showed the hypervirulence phenotype, all of them presented important virulence factors and genetic diversity. A small number of chains of microbial transmission apparently related to biofilm production evidenced the persistence and transfer of multiresistant CRKP strains in the hospital environment.
SELF-HEATING MODELING BASED ON THE THERMOGRAVIMETRIC ANALYSIS METHOD FOR STOCKPILE MANAGEMENT IN LIGNITE-A COAL STOCKPILE MANAGEMENT
Authors: Nursanto Edy , Prasetyo Dessyanto Boedi , Supriyanta Bambang , Suharyadi Heru , Supandi  , Fadhilah Rahimatul
Published: January 2025

The storage and management of lignite coal stockpiles in the mining industry face significant challenges related to self-heating and spontaneous combustion risks, which can cause safety hazards and financial losses. The physical and chemical characteristics of lignite contribute to its oxidation process, which can lead to an increase in temperature. This study aimed to use thermogravimetric analysis (TGA) to create a self-heating model of lignite-A coal from the Warukin Formation and propose a mitigation strategy. Coal samples aged 0 days, 7 days, and 14 days were analyzed to determine their chemical and physical composition, while TGA and COMSOL modeling were used to monitor the temperature dynamics and distribution of chemical species in the stockpile for 30 days. The results of the study explain that water content and chemical components play a significant role in the potential for self-heating, where lignite-A with a moisture content of 38% on an as-receiving basis shows a greater risk. The simulations show a significant increase in temperature from 400 K to 458 K in certain areas, potentially triggering spontaneous combustion. In conclusion, the management of the self-heating risk of lignite-A coal can use coal characteristic data, such as moisture content (38%), carbon (71.9%), hydrogen (5.1%), and oxygen (21.5%), as well as modeling results, for 30 days to develop more effective mitigation strategies and improve storage safety and efficiency.