bluuebunny/crossref_metadata_2025 huggingface dataset:
Measuring the Dimension Accuracy of Products Created by 3D Printing Technology with the Designed Measuring System
Authors: Pollák Martin , Sabol Dominik , Goryl Karol
Published: December 2024

The integration of precision measurement techniques using depth scanners with PLC control provides new possibilities for increasing the efficiency and quality of measuring 3D printed products. Comprehensive analysis of measurements in combination with advanced algorithms can provide more accurate dimensional characteristics and the prediction of defects. The overall goal of such systems is to assess the accuracy and reliability of products manufactured by 3D printing technology, which will fundamentally affect their increased use in industrial sectors. The article describes the design and implementation of a control system for a servo drive used in the proposed solution of a measuring system with a depth sensor, primarily oriented to the control and precise measurement of manufactured products by 3D printing technology. The entire program plan is implemented through the CCW software ver. 12 (Connected Component Workbench) and micro motion control devices. The connection of the servo drive itself is realized by combinations of the servo drive with the HMI (Human Machine Interface) panel and the servo drive with the PLC controller. It is the PLC controller that is subject to the created program in the CCW software, which can be used to control the direction, position, speed, and acceleration of the servomotor. The result is the design of a measurement scanning system with precise motion control through a connected servo drive intended for industrial applications of object error detection and measurement of dimension accuracy.
Classification of Fungal Pigments by Simulating Their Optical Properties Using Evolutionary Optimization
Authors: Pishchalnikov Roman Y. , Chesalin Denis D. , Kurkov Vasiliy A. , Razjivin Andrei P. , Gudkov Sergey V. , Grishin Andrey A. , Dorokhov Alexey S. , Izmailov Andrey Yu.
Published: December 2024

Modern developments in data analysis techniques and evolutionary optimization algorithms have made it possible to analyze large amounts of unstructured digital data sets. Based on the differential evolution algorithm and semiclassical quantum simulations, we have recently proposed a method for classifying and analyzing the optical properties of organic pigments. In this paper, we present the results of modeling the absorption spectra of five carotenoids synthesized during the vital activity of the ascomycetous fungi: neurosporaxanthin, neurosporene, torulene, γ-carotene, and ζ-carotene. We calculated the absorption spectra for each pigment using the multimode Brownian oscillator theory, which allows us to evaluate the influence of molecular vibrations on the electronic transitions in the pigment. We applied a generalized spectral density function method to our modeling, taking into account the contributions of 13 vibrational modes with frequencies varying between 100 cm−1 and 3000 cm−1. This approach allowed us to gain a deeper understanding of how molecular vibrations affect the absorption spectra of these organic compounds. Thus, each absorption spectrum was associated with a unique set of Huang–Rhys factors (which represent the effective electron–phonon interaction). This set can be considered as a kind of “fingerprint” that characterizes the optical response of the pigment in the solvent.
Bacillus lumedeiriae sp. nov., a Gram-Positive, Spore-Forming Rod Isolated from a Pharmaceutical Facility Production Environment and Added to the MALDI Biotyper® Database
Authors: Costa Luciana Veloso da , Ramos Juliana Nunes , Albuquerque Leticia de Sousa , Miranda Rebeca Vitória da Silva Lage de , Valadão Talita Bernardo , Veras João Flávio Carneiro , Vieira Erica Miranda Damasio , Forsythe Stephen , Brandão Marcelo Luiz Lima , Vieira Verônica Viana
Published: December 2024

A Gram-positive, aerobic, rod-shaped and spore-forming bacterium strain designation, B190/17, was isolated from an air monitoring sample of a Brazilian immunobiological production facility in 2017. The strain was not identifiable by biochemical methodology VITEK® 2 or by MALDI-TOF MS with VITEK® MS RUO and MALDI Biotyper®. The 16S rRNA gene sequencing results showed 98.51% similarity with Bacillus wudalianchiensis FJAT 27215T, 98.28% with ‘Bacillus aerolatus’ CX 253T, 97.96% with Bacillus badius MTCC 1458T, 97.63% with Bacillus xiapuensis FJAT 46582T and 97.21% with Bacillus thermotolerans SGZ8T. Biochemical data showed that the strain was alanine arylamidase-, Ala-Phe-Pro arylamidase-, ELLMAN (cysteine residues)-, leucine arylamidase-, phenyalanine arylamidase- and tyrosine arylamidase-positive. The genomic DNA G+C% content of B190/17 was 41.6 mol%. The phylogenetic, genomic taxonomy and biochemical tests suggested that B190/17 represents a novel species and should be classified as the type strain of a novel Bacillus species. The name Bacillus lumedeiriae sp. nov. was proposed. After characterization, B190/17 was added to the MALDI Biotyper® database as Bacillus lumedeiriae sp. nov.
Multi-Analytical Approach for the Acid-Base, Thermal and Surface Properties Assessment of Waste Biomasses
Authors: Raccuia Salvatore Giovanni Michele , Zanda Emanuele , Bretti Clemente , Formica Mauro , Macedi Eleonora , Melchior Andrea , Tolazzi Marilena , Sanadar Martina , Lascari Davide , De Luca Giovanna , Irto Anna , De Stefano Concetta , Cardiano Paola , Lando Gabriele
Published: December 2024

A multi-analytical approach was used to comprehensively characterize the acid-base, thermal, and surface properties of agri-food processing wastes (i.e., original and pre-treated bergamot, grape and olive pomaces). These biomasses, often underutilised and inadequately studied in terms of their physicochemical properties, were investigated under varying ionic strength conditions at t = 25 °C. This investigation uniquely integrates multiple advanced techniques: Brunauer–Emmett–Teller porosimetry, Scanning Electron Microscopy, Thermogravimetric Analysis coupled with Fourier Transform Infrared Spectroscopy, Differential Scanning Calorimetry, Attenuated Total Reflectance Fourier-Transform Infrared, and potentiometry to provide a holistic understanding of these biomasses potential for environmental remediation. The modelling of ionic strength-dependent acid-base behaviour, established using an extended Debye–Hückel-type equation, revealed the dominant role of carboxylic groups as active sites across all pomace types, although with variations in abundances across the different samples. Additionally, morphological analysis highlighted the presence of irregularly shaped particles, heterogeneous size distributions, and distinct thermal stability trends, with grape pomace exhibiting the highest mass loss. These findings underscore the significant potential of these biomasses for the remediation of cationic pollutants from natural waters. Moreover, this comprehensive characterisation not only advances the understanding of agri-food waste valorisation but also provides a robust framework for designing targeted strategies in environmental applications.
Impacts of Vinyl Group on the Diffusion and Thermal Properties of Branched Silicon-Containing Arylacetylene Resins by Molecular Dynamics Simulations
Authors: Li Hui , Sun Zijian , Yang Lei , Zhu Weihua
Published: December 2024

Branched-structure polyarylacetylene resins with low curing temperatures and excellent thermal stability are promising candidates for high-performance resin-matrix composites. In this work, the effects of different vinyl/acetylene group ratios on the thermal properties of star-shaped silicon-containing arylacetylene (SSA) resins and branched silicon-containing arylacetylene (BSA) resins were investigated by molecular dynamics simulations. The calculated interaction energies, diffusion behaviors, and glass transition temperatures (Tg) indicated that an increase in the vinyl/acetylene group ratio positively affected the rheological and thermal properties of the branched resins. After the introduction of the vinyl groups, the Tg value of SSA2 was greater than that of SSA0. An energy decomposition approach (EDA) analysis further confirmed that the involvement of the vinyl groups in the BSA and SSA resins provided larger interaction energies dominated by dispersion effects, leading to better thermal performance. This work may provide us with a deep understanding of the incorporation of vinyl groups in silicon-containing arylacetylene resins.
Rational Design of V−ZnCo2O4 Nanowires on Nickel Foam: Achieving Superior Capacitance and Mechanical Resilience
Authors: Li Yucai , Song Shiwei , Dai Meizhen , Wang Jian , Ke Yunjie , Zhang Dong , Liu Wenjun , Luo Guan
Published: December 2024

The structural characteristics of electrode materials play a crucial role in their potential applications. Therefore, designing the material’s structure rationally is one of the most effective methods to achieve high-performance electrodes. In this study, V−ZnCo2O4 nanowires were synthesized on nickel foam using a simple hydrothermal method, and the prepared V−ZnCo2O4−2 electrode material exhibited a specific capacitance of 1621 C g−1. The potential applications of the prepared material were evaluated through device assembly, using V−ZnCo2O4−2 as the positive electrode and activated carbon as the negative electrode. The resulting device delivered an energy density of 127.5 Wh/kg, with a corresponding power density of 2700 W/kg. Additionally, the mechanical properties of the device were assessed, revealing that after multiple bends at different angles, the shape of the device remained well-preserved, further confirming its excellent mechanical stability.
Synthesis of Enantiostructured Triacylglycerols Possessing a Saturated Fatty Acid, a Polyunsaturated Fatty Acid and an Active Drug Intended as Novel Prodrugs
Authors: Jónsdóttir Lena Rós , Haraldsson Gudmundur G.
Published: December 2024

This report describes the asymmetric synthesis of a focused library of enantiopure structured triacylglycerols (TAGs) comprised of a single saturated fatty acid (C6, C8, C10, C12, C14 or C16), a pure bioactive n-3 polyunsaturated fatty acid (EPA or DHA) and a potent drug (ibuprofen or naproxen) intended as a novel type of prodrug. One of the terminal sn-1 or sn-3 positions of the glycerol backbone is occupied with a saturated fatty, the remaining one with a PUFA, and the drug entity is present in the sn-2 position. This was accomplished by a six-step chemoenzymatic approach starting from enantiopure (R)- and (S)-solketals. The highly regioselective immobilized Candida antarctica lipase (CAL-B) played a crucial role in the regiocontrol of the synthesis. All combinations, a total of 48 such prodrug TAGs, were prepared, isolated and fully characterized, along with 60 acylglycerol intermediates, obtained in very high to excellent yields.
Cytotoxic and Antibacterial Cyclodepsipeptides from an Endophytic Fungus Fusarium avenaceum W8
Authors: Wang Zimo , Liu Bo , Wang Yanlei , Xu Yicen , Ma Hai , Sun Yi
Published: December 2024

Seven cyclic depsipeptides, including two new cyclic pentadepsipeptides avenamides A (1) and B (2), were isolated from a plant-derived fungus Fusarium avenaceum W8 by using the bioassay-guided fractionation method. The planar structures were elucidated by using comprehensive spectroscopic analyses, including 1D and 2D NMR, as well as MS/MS spectrometry. The absolute configuration of the amino acid and hydroxy acid residues was confirmed by using the advanced Marfey’s method and chiral HPLC analysis, respectively. Compounds 1–7 were evaluated for their cytotoxic activities against A549 and NCI-H1944 human lung adenocarcinoma cell lines and their antimicrobial activities against Staphylococcus aureus and Saccharomyces cerevisiae. As a result, compounds 1–4 showed moderate cytotoxicity, with IC50 values of 6.52~45.20 µM. Compounds 1 and 3 exhibited significant antimicrobial activities against S. aureus and S. cerevisiae, with an MIC80 of 11.1~30.0 µg/mL.
The Formation of γ-Valerolactone from Renewable Levulinic Acid over Ni-Cu Fly Ash Zeolite Catalysts
Authors: Popova Margarita , Boycheva Silviya , Dimitrov Ivan , Dimitrov Momtchil , Kovacheva Daniela , Karashanova Daniela , Velinov Nikolay , Atanasova Genoveva , Szegedi Agnes
Published: December 2024

Zeolites with different structures (P1, sodalite, and X) were synthesized from coal fly ash by applying ultrasonically assisted hydrothermal and fusion–hydrothermal synthesis. Bimetallic catalysts, containing 5 wt.% Ni and 2.5 wt.% Cu, supported on the zeolites, were prepared by a post-synthesis incipient wetness impregnation method. The catalysts were characterized by X-ray powder diffraction (XRPD), N2 physisorption, transmission electron microscopy (TEM), Mössbauer and X-ray photoelectron spectroscopies (XPS), and H2–temperature-programmed reduction (H2-TPR) analyses. The XRPD results showed that crystalline Cu0 and NixCuy intermetallic nanoparticles were formed in the reduced catalysts. The presence of the intermetallic phase affected the reducibility of the nickel by shifting it to a lower temperature, as confirmed by the H2-TPR curves. Based on the Mössbauer spectroscopic results, it was established that the iron contamination of the coal fly ash zeolites (CFAZs) was distributed in ionic positions of the zeolite lattice and as a finely dispersed iron oxide phase on the external surface of the supports. The formation of the NiFe alloy, not detectable by XRPD, was also evidenced on the impregnated samples. The catalysts were studied in the upgrading of levulinic acid (LA), derived from lignocellulosic biomass, to γ-valerolactone (GVL), in a batch reactor under 30 bar H2 pressure at 150 and 200 °C, applying water as a solvent. The NiCu/SOD and NiCu/X catalysts showed total LA conversion and a high GVL yield (>75%) at a reaction temperature of 200 °C. It was found that the textural parameters of the catalysts have less influence on the catalytic activity, but rather the stable dispersion of metals during the reaction. The characterization of the spent catalyst found the rearrangement of the support structure. The high LA conversion and GVL yield can be attributed to the weak acidic character of the support and the moderate hydrogenation activity of the Ni-Cu sites with high dispersion.
Synthesis and Characterisation of Phosphino-Aryloxide Rare Earth Complexes
Authors: Alexopoulos Elias , Liu Yu , Bowles Alex W. J. , Réant Benjamin L. L. , Ortu Fabrizio
Published: December 2024

A series of homoleptic rare earth (RE) complexes bearing phosphino-aryloxide ligands (1-RE, 2-La) has been prepared. The complexes have been characterised using multinuclear NMR and IR spectroscopy, X-ray crystallography and elemental analysis. Structural characterisation highlighted the different RE–P interactions as a result of differing Lewis acidity and ionic size across the series, hinting at the possibility of FLP-type activity. The potential reactivity of these complexes has been tested by reacting them with small molecules (H2, CO, CO2). A series of side-products (3-RE) has also been observed, isolated and characterised, featuring the incorporation of a phosphonium-aryloxide ligand.
Cluster Headache and Hypoxia: Breathing New Life into an Old Theory, with Novel Implications
Authors: Borkum Jonathan M.
Published: December 2024

Cluster headache is a severe, poorly understood disorder for which there are as yet virtually no rationally derived treatments. Here, Lee Kudrow’s 1983 theory, that cluster headache is an overly zealous response to hypoxia, is updated according to current understandings of hypoxia detection, signaling, and sensitization. It is shown that the distinctive clinical characteristics of cluster headache (circadian timing of attacks and circannual patterning of bouts, autonomic symptoms, and agitation), risk factors (cigarette smoking; male gender), triggers (alcohol; nitroglycerin), genetic findings (GWAS studies), anatomical substrate (paraventricular nucleus of the hypothalamus, solitary tract nucleus/NTS, and trigeminal nucleus caudalis), neurochemical features (elevated levels of galectin-3, nitric oxide, tyramine, and tryptamine), and responsiveness to treatments (verapamil, lithium, melatonin, prednisone, oxygen, and histamine desensitization) can all be understood in terms of hypoxic signaling. Novel treatment directions are hypothesized, including repurposing pharmacological antagonists of hypoxic signaling molecules (HIF-2; P2X3) for cluster headache, breath training, physical exercise, high-dose thiamine, carnosine, and the flavonoid kaempferol. The limits of current knowledge are described, and a program of basic and translational research is proposed.
Performance Improvement by FRFT-OFDM for Visible Light Communication and Positioning Systems
Authors: Li Wenyang , Wang Zixiong , Yu Jinlong
Published: December 2024

In indoor visible light communication (VLC) and visible light positioning (VLP) systems, the performance of conventional orthogonal frequency-division multiplexing (OFDM) schemes is often compromised due to the nonlinear characteristics and limited modulation bandwidth of light-emitting diodes, the multipath effect in enclosed indoor environments, and the relative positions of transmitters and receivers. This paper proposes an OFDM scheme based on the fractional Fourier transform (FRFT) to address these issues, demonstrating promising results when applied to indoor VLC and VLP systems. The FRFT, a generalization of the conventional Fourier transform (FT) in the fractional domain, captures information in both the time and frequency domains, offering greater flexibility than the FT. In this paper, we first introduce the computation method of the reality-preserving FRFT for an intensity modulation/direct detection VLC system and integrate it with OFDM to optimize system performance. By adopting FRFT-OFDM under the optimal fractional order, we enhance both the bit error ratio (BER) performance and positioning accuracy. Simulation results reveal that the FRFT-OFDM scheme with an optimized fractional order significantly improves the BER and positioning accuracy compared to the FT-OFDM scheme across most receiver positions within the indoor observation plane. For communication, the FRFT-OFDM scheme achieves over 6 dB Eb/N0 gain compared to the FT-OFDM scheme at a BER of 3×10−4 when the receiver is positioned at most locations in the room. For positioning, the FRFT-OFDM scheme enhances positioning accuracy by more than 1 cm relative to the FT-OFDM scheme at most locations in the room. Notably, both systems maintain the same computational complexity and spectral efficiency.
A Novel Temporal Fusion Channel Network with Multi-Channel Hybrid Attention for the Remaining Useful Life Prediction of Rolling Bearings
Authors: Wang Cunsong , Jiang Junjie , Qi Heng , Zhang Dengfeng , Han Xiaodong
Published: December 2024

The remaining useful life (RUL) prediction of rolling bearings is crucial for optimizing maintenance schedules, reducing downtime, and extending machinery lifespan. However, existing multi-channel feature fusion methods do not fully capture the correlations between channels and time points in multi-dimensional sensor data. To address the above problems, this paper proposes a multi-channel feature fusion algorithm based on a hybrid attention mechanism and temporal convolutional networks (TCNs), called MCHA-TFCN. The model employs a dual-channel hybrid attention mechanism, integrating self-attention and channel attention to extract spatiotemporal features from multi-channel inputs. It uses causal dilated convolutions in TCNs to capture long-term dependencies and incorporates enhanced residual structures for global feature fusion, effectively extracting high-level spatiotemporal degradation information. The experimental results on the PHM2012 dataset show that MCHA-TFCN achieves excellent performance, with an average Root-Mean-Square Error (RMSE) of 0.091, significantly outperforming existing methods like the DANN and CNN-LSTM.
Mini-Review on the Design Principles of Biochemical Oscillators for the Continuous Ethanol Fermentation Processes
Authors: Zhai Chi , Ji Cheng , Sun Wei
Published: December 2024

Computational modeling and the theory of nonlinear dynamical systems allow us not only to simply describe the events of biochemical oscillators in the ethanol fermentation process but also to understand why these events occur. This article reviews results of experimental and theoretical studies about the behavior of fermentation systems for bio-ethanol production so as to understand the self-oscillatory phenomena that could affect productivity in industry. In general, Hopf bifurcation and limit cycles are the theoretical basis for the oscillations observed in continuous ethanol fermentation processes, but the underline mechanisms and causes might be different because the studied system is a collection of multi-scale oscillators. To characterize the oscillatory dynamics quantitatively, negative feedback laws are implemented. However, the stimulated oscillation through linear feedback is not adequate in describing such complex dynamics. Hence, elements of nonlinearity, auto-catalysis, and time delay are sorted out and added into the feedback loops to formulate biochemical oscillators. Then, we discuss specific examples of the various models and classify them according to the three kinds of mechanisms: nonlinear feedback, positive feedback, and delay feedback. These mechanisms and modeling work might be used as a guide for process design/operation to eliminate possible oscillations and to develop out advanced configurations that could produce bio-ethanol in a continuous, cost-effective manner.
Interactions of Publication Volume, Journal Impact, and Article Processing Charges: Comparative Study of China and Global Practices in Nature Portfolio
Authors: Chen Xinyi
Published: December 2024

In the commercial realm, the annual publication volume (PUB) significantly influences the business models of article processing charge (APC)-based open access (OA) journals, though it may negatively impact journal reputation. Despite this, the interactions among APC, PUB, and the Journal Citation Indicator (JCI)—a key marker of journal reputation—have not been thoroughly examined. The objective of this study is to reveal the interactions among APC, PUB, and JCI, determine if there are differences between the interactions inside and outside of China, and uncover the possible mechanisms enabling dominant publishers to set APC prices without compromising their market position. Through cross-correlation and linear regression analyses, our findings reveal distinct APC business models between China and the rest of the international OA journal landscape. Specifically, while both cases demonstrate a proportional relationship between APC and JCI, China exhibits an inverse relationship between APC and PUB, contrasting with the global trend. This suggests that the business model in China sets an “optimized” PUB for Chinese APC-based OA journals, which would pose challenges for journal management and the expansion of the domestic APC-based OA market volume. In the rest of the international context, by contrast, the business model supports a proactive annual increase in APC list prices driven by the proportional relationships between APC and PUB. These insights underscore the need for more nuanced APC business models that can adapt to regional variations in funder requirements and policy expectations.
Waste Minimization of Lead Paste and Jarosite to Recover a Silver-Rich Alloy by the Pyrometallurgical Route
Authors: Sanchez Vite Jose Enrique , Cruz Ramírez Alejandro , Flores Favela Manuel Eduardo , Romero Serrano José Antonio , Pérez Labra Miguel , Gutiérrez Pérez Víctor Hugo , Sánchez Alvarado Ricardo Gerardo , Jiménez Lugos Juan Cancio
Published: December 2024

A silver-rich lead alloy was obtained through the recycling of two metallurgical wastes: these are lead paste obtained from spent lead–acid batteries and a jarosite residue obtained from the hydrometallurgical production of zinc. Mixtures of both wastes were pyrometallurgically treated with sodium carbonate in a silicon carbide crucible at 1200 °C. The alloy and slag produced were analyzed by atomic absorption spectrometry, X-ray diffraction, and scanning electron microscopy with energy-dispersive spectra. High silver recovery was obtained in a Pb-Ag alloy for a mixture ratio of 30% Na2CO3–40% lead paste–30% jarosite, reaching a silver grade of 126 ppm. The slags produced for the highest jarosite content allow the compound formation of Na2(SO4) and Na2Fe(SO4)2, which have high sulfur-fixing, avoiding SO2 release and contributing to the minimization of atmospheric pollution. The novel pyrometallurgical route addresses not only the valorization of precious metals such as silver and lead but also the reduction in accumulated industrial waste.
The 100-Days: Physical Exercise and Challenges to Assess, Maintain and Improve Physical Fitness During Lockdown
Authors: Di Libero Tommaso , D’Ermo Annalisa , Tosti Beatrice , Corrado Stefano , Diotaiuti Pierluigi , Rodio Angelo
Published: December 2024

The COVID-19 pandemic reduced physical activity and increased sedentary behavior, raising health risks. To combat this, a 100-day training program was designed to maintain and improve fitness during lockdown. This program, which included a challenge with physical assessments and online sessions, aimed to enhance fitness and motivation. Methods: Twenty participants (mean age 45.2 ± 12.7 years) with good baseline fitness completed strength, endurance, coordination, and flexibility exercises over 100 days, with monthly challenges. Fitness was assessed at baseline (T0), mid-program (T1), and completion (T2). Results: Males initially showed higher BMI and mild cardiovascular risks. Flexibility improved for both genders, with females increasing from 12.5 ± 4.51 cm to 14.8 ± 6.65 cm and males from 4.4 ± 6.33 cm to 8.8 ± 10.69 cm. Males’ vertical jump height increased from 20.7 ± 13.05 cm to 28.2 ± 10.49 cm. In the challenge, push-up repetitions rose for both genders, achieving excellent scores (>24 reps for females, >25 for males). Males advanced in the repeated crunch test, while females consistently excelled in the repeated squat. Weight and BMI reductions were also observed, particularly in overweight males. Conclusions: The 100-day training program, combined with the challenge, effectively sustained physical fitness and motivation among participants during pandemic-related restrictions. Notable strength and endurance improvements were observed across both genders, reinforcing the potential of interactive, remote training programs to promote physical health in periods of limited activity.
The Influence of Plant Growth-Stimulating Bacteria on the Glutathione-S-Transferase Activity and the Toxic Effect of the Herbicide Metsulfuron-Methyl in Wheat and Canola Plants
Authors: Chetverikova Darya , Bakaeva Margarita , Starikov Sergey , Kendjieva Aliya , Chetverikov Sergey
Published: December 2024

The ability of some rhizosphere bacteria to mitigate herbicidal stress in cultivated plants may be useful in agriculture and bioremediation. There is poor understanding of how bacteria directly or through herbicide degradation affect the biochemical processes in plants exposed to sulfonylurea herbicides. In this study, treatment with a combination of herbicide metsulfuron-methyl (MSM) and bacteria (Pseudomonas protegens DA1.2 or P. chlororaphis 4CH) of wheat (Triticum aestivum L.) and canola (Brassica napus L.) plants was carried out. Activity of glutathione-S-transferase (GST), an important enzyme for the herbicide detoxification, and acetolactate synthase (ALS), a target for MSM in plants, was measured by spectrophotometric assays. MSM residues were analyzed using the HPLC-MS. Then, 24 h after bacterial treatment, GST activity increased by 75–91% in wheat and by 38–94% in canola. On the 30th day, a decrease in MSM in the soil associated with bacterial treatment was 54.6–79.7%. An increase in GST activity and acceleration of MSM degradation were accompanied by a decrease in inhibition of the ALS enzyme in plants, which indicated a mitigation of the toxic effect. The results obtained are evidence that rhizospheric bacteria can have beneficial effects on plants exposed to MSM due to the combination of abilities to directly affect detoxification enzymes in plants and degrade MSM in the soil.
Ehrlichia canis Vaccine Development: Challenges and Advances
Authors: Alves-Ribeiro Bruna Samara , Duarte Raiany Borges , Assis-Silva Zara Mariana de , Gomes Ana Paula Carvalho , Silva Yasodaja Assis , Fernandes-Silva Lizandra , Rocha Alice Caroline da Silva , Moraes Iago de Sá , Saturnino Klaus Casaro , Ramos Dirceu Guilherme de Souza , Taques Isis Indaiara Gonçalves Granjeiro , Braga Ísis Assis
Published: December 2024

Canine monocytic ehrlichiosis (CME) is an infectious disease caused by Ehrlichia canis, a globally recognized obligate intracellular bacterium. In addition to dogs, other animals, including humans, may be affected. Despite its epidemiological importance and impact on public health, there is currently no commercial vaccine against E. canis. This study aimed to present relevant aspects of the challenges and advances encountered in the development of vaccines for CME and highlight perspectives for future investigations. High genetic variability, along with the various evasion mechanisms employed by E. canis, has hindered the identification of an antigen that targets Th1 cells and is immunogenic to most E. canis isolates, considering their genotypic and phenotypic characteristics. The vaccine must predominantly confer cellular and humoral immunity to achieve robust immune responses. Early production efforts have been challenging due to low immunogenicity, difficulties in establishing long-term protection, and limitations of the techniques used. However, with the refinement of bioinformatic tools, research in this area will be facilitated, thereby accelerating the development of effective vaccines for CME. According to these authors, this vaccine should consist of multiple epitopes.
Assessment of the G model adopted in maintenance management within the oil sector institutions in Skikda (Sonatrach) and its role in enhancing maintenance operations
Authors: Bouaninba Wahiba , Zohra Saad Guermeche
Published: December 2024

This study delves into the maintenance model G adopted by the maintenance management within the oil sector institutions in Skikda (Sonatrach). Recognized as a strategic autonomous entity that encompasses everything from planning to control, this management sector is pivotal in maximizing and leveraging available production energies based on scientific diagnostics and structured execution. This is instrumental in achieving reduced maintenance costs. Moreover, the study seeks to implement this model within a comprehensive maintenance framework that encapsulates the essential pillars of maintenance management: robust organization, effective human resource management, clear objectives and strategies, along with the support and commitment of top management. A field study examining the various phases of implementing preventive and corrective maintenance using the G model revealed that this function often lacks the requisite emphasis and care. This is attributed to the maintenance management's failure to effectively implement the G model as stipulated by the company, a deficient awareness in activating maintenance management perceived merely as a cost-reduction tool, coupled with a neglect of the human resources pillar and the lack of support and commitment from senior management.
Avaliação histopatológica, hematológica e bioquímica dos efeitos da ozonioterapia na inflamação induzida por carragenina no músculo gastrocnêmio de ratos wistar
Authors: Tremador Gabriela Aparecida Machado , Gomes Maria Julia de Matos , Silva Mayla Koglim da , Ramos Orivaldo Pereira , Gualberto Maria Julia de Oliveira Santos , Bailão Silvia Zucchi , Junior José Roberto Cunha , Crisci Ana Rosa
Published: December 2024

A Ozonioterapia tem sido objeto de estudo multidisciplinar por suas propriedades microbicidas, anti-inflamatórias e terapêuticas que podem ser aplicadas na área da saúde, permitindo ser administrada por diversas vias, tanto com o uso isolado do insumo ou como coadjuvante a outros tratamentos. Sendo um gás natural, não apresenta relatos de efeitos adversos, sendo uma opção viável quando comparado a medicamentos normalmente utilizados. Esse trabalho visou analisar os efeitos da ozonioterapia no processo inflamatório do músculo gastrocnêmio, induzido pela Carragenina, por meio da avaliação das enzimas TGO, TGP, ureia e creatinina e a velocidade de hemossedimentação (VHS) e da analise histopatologia do músculo e dos tecidos subjacentes. Foram analisados um total 32 ratos da espécie Rattus novergicus de linhagem Wistar, fêmeas, pesando em média 300g, que foram divididos em quatro grupos, grupo 1: tratamento com salina (controle negativo); grupo 2: ozônio intramuscular; grupo 3: tratamento medicamentoso com celecoxibe por gavagem (controle positivo) e grupo 4: gavagem de celecoxibe + ozônio intramuscular, para realizar a comparação. Os resultados do VHS embora não foram estatisticamente significativos (P=0,5) os grupos tratados foram bem menores comparados com o grupo controle, indicando uma menor inflamação. As dosagens enzimáticas demonstraram prejuízos hepáticos e renais em todos os tratamentos. Na histopatologia, as fibras musculares e tecido conjuntivo adjacente apresentaram-se mais preservados no grupo tratado com celebra e ozônio. Concluiu-se que a ozonioterapia foi promissora em alguns parâmetros enquanto em outros apontou a necessidade de mais estudos, tendo um melhor aproveitamento quando utilizada como um tratamento coadjuvante.
Influência da humanização em relação a percepção dos tutores sobre o escore corporal dos cães
Authors: Didio Helena Pinto , Hauptli Lucélia , Lima André Luis Ferreira , Moraes Priscila de Oliveira , Alves Paola de Freitas , Lauterjung Ana Carolina , Pereira Aretha Helena , Santos Pietra de Franca Belfort
Published: December 2024

O objetivo deste trabalho foi investigar junto a tutores, a humanização dos cães e sua influência na percepção do escore de condição corporal de cães (ECC), por meio de um questionário. O estudo foi desenvolvido na cidade de Florianópolis – SC. O questionário foi composto por perguntas que abordaram o perfil do tutor, do cão e aspectos de humanização do tutor com o cão. Foi aplicado de forma presencial em locais públicos com notória presença de tutores com seus cães. Foram obtidas 389 respostas. As respostas foram submetidas a análise descritiva. A análise comparativa da percepção de escore da condição corporal do cão (Escore 1 – Muito magro; 2 – Magro; 3 – Ideal; 4 – Sobrepeso; 5 – Obeso) por parte do seu tutor em relação a percepção do entrevistador, foi realizada por teste não-paramétrico de Spearman, para obtenção dos coeficientes de correlação. Sobre o perfil dos tutores, a maioria eram mulheres (66,1%), na faixa etária de 26 a 46 anos (62%) e praticantes de atividades físicas regulares (75,8%), sendo que 49,9% se consideravam no peso ideal e 46,3% acima do peso. Em relação aos cães: 53% eram machos e 47% fêmeas; 28,5% eram sem raça definida (SRD) e a maioria com porte pequeno a médio (88,9%). A faixa etária predominante foi de 1 a 4 anos (50,1%). De acordo com os tutores 70,1% dos cães praticam atividades física intensa a moderada. A correlação entre o entrevistador e o tutor (que foi entrevistado), mostraram concordância significativa (p<0,01) entre as respostas de ambos sobre o ECC do cão avaliado. Onde houve predominância (média de 69%) de escore de condição corporal 3, que é peso ideal. A maioria dos tutores (76,3%) não criou redes sociais a seus cães, não frequentam eventos sociais voltados a cães (74,6%) e os que possuem roupas são, na maioria, para situações de proteção de frio. Considerando somente as 25,89% respostas dos tutores, que não apresentaram concordância entre a observação de escore de condição corporal dos cães comparadas ao entrevistador, a maior parte dos atributos relacionados a humanização não ocorrem. Onde 70,40% destes cães não possuem fantasias e acessórios, e 84,69% não participam de eventos voltados a cães e não possui redes sociais, o que demonstra que a humanização não influencia a percepção do tutor do escore de condição corporal do cão. Logo, não há alta ocorrência de humanização dos cães de Florianópolis – SC. Não havendo influência deste fator na percepção dos tutores sobre o escore corporal dos cães, os quais predominantemente estavam e ECC de 3, considerado peso ideal.
Proposta de redução da área de operação de sistemas de chuveiros automáticos com alteração de densidade
Authors: Fernandes Ivan Ricardo
Published: December 2024

Os sistemas de chuveiros automáticos são utilizados em todo o mundo para a preservação do patrimônio e tem contribuído para a diminuição das taxas de mortalidade em incêndios. Dados da NFPA revelaram que cinco ou menos chuveiros automáticos foram ativados em 96% dos incêndios, e ainda que, quanto menor a quantidade de chuveiros acionados maior é a eficiência no combate ao fogo. A NBR 10897 tem sido adotada pelos Corpos de Bombeiros Militares como parâmetro de cálculo hidráulico do sistema, em função de um número restrito de chuveiros automáticos na chamada área de operação. O objetivo deste trabalho foi apresentar uma proposta de redução da área de operação de chuveiros automáticos com alteração de densidade. Por meio da utilização de um método dedutivo e cálculos matemáticos baseados na equação da reta, foi possível realizar simulações computacionais que permitiram a comparação dos resultados com a redução das áreas de operação. Os resultados demonstraram que a redução da área de operação resulta na diminuição dos valores de vazão, pressão e volume do reservatório, com perspectiva de redução de custos para implantação do sistema. Ao final conclui-se pela possibilidade de exigência do sistema em função da área das edificações, e a adoção de uma nova curva densidade área em substituição a atualmente utilizada na NBR 10897.
Avaliação microbiológica de frutas e hortaliças minimamente processadas e in natura comercializadas em supermercado de Marília – SP
Authors: Portugal Luana Seleguim , Muzzi Elaine Cristina Alves dos Santos , Shigematsu Elke , Giannoni Juliana Audi , Dorta Claudia , Farinazzi-Machado Flavia Maria Vasques , Góes-Favoni Silvana Pedroso de , Pardo Renata Bonini
Published: December 2024

O trabalho avaliou a qualidade microbiológica de frutas e hortaliças minimamente processadas, in natura e desidratas, identificando e quantificando a presença de mesófilos aeróbios, bolores e leveduras e a família Enterobacteriaceae, verificando um comparativo da data de fabricação e a vida de prateleira de até seis dias. Foram realizadas coleta de seis amostras de frutas e hortaliças, sendo laranja desidratada, salada de frutas, melão in natura, melão minimamente processado, “suco detox” com frutas e hortaliças minimamente processadas. As amostras foram recolhidas aleatoriamente das gôndolas refrigeradas de um supermercado localizado em Marília/SP. As amostras foram semeadas em meios de cultura específicos para análises de bolores e leveduras, mesófilos aeróbios e a família Enterobateriaceae. Observou-se que das 6 amostras de frutas e hortaliças minimamente processadas e in natura, os mesófilos aeróbios foram identificados em 100% das amostras analisadas, porém não há legislação vigente e específica do limites para estes microrganismos para frutas e hortaliças minimamente processados e in natura no Brasil. Na análise de bolores e leveduras pode-se observar que a laranja desidratada apresentou conformidade, de acordo com os parâmetros vigentes da Instrução Normativa n°161 de 2022, os demais alimentos minimamente processados, a legislação não determina limites estabelecidos para bolores e leveduras. Através destas análises, conclui-se que o melão in natura, o “suco detox” com frutas e hortaliças minimamente processadas com um dia de vida de prateleira e a laranja desidratada apresentaram os melhores resultados microbiológicos para comercialização.
Avaliação da eficácia de dentifrício clareador com corante Blue Covarine
Authors: Albuquerque Andrey dos Santos , Silva Layla Gabrielli Vasconcelos , Silva Ana Maria Catônio da , Filho Hibernon Lopes Lima , Romão Dayse Andrade , Vasconcelos Rafaela Andrade de , Silva Marcos Aurélio Bomfim da , Rodrigues Raphaela Farias
Published: December 2024

O blue covarine é um elemento clareador que induz transformações visuais na superfície dos dentes, combinado com os componentes abrasivos presentes nos cremes dentais, contribuindo para o clareamento dos dentes. O objetivo deste estudo foi comparar a eficácia clareadora de dentifrícios com blue covarine e sem agente clareador. Trata-se de uma pesquisa acadêmica onde foram utilizados mm total de 39 dentes bovinos, divididos em 3 grupos (n=13): WNGF (dentifrício com corante blue covarine; Closeup), C10 (Gel de peróxido de carbamida 10% - Whiteness Perfect - FGM - Brasil) e TA (dentifrício convencional Tripla Ação; Colgate). Os dentes foram escurecidos em solução de chá preto até atingir a cor A4 da escala Vita Classical. Estes grupos foram submetidos a 17.000 ciclos de escovação. Foi verificada a cor inicial e final das amostras, por um examinador cego e previamente calibrado, utilizando a escala Vita Classical. Os tons registrados foram convertidos em pontuações para comparação da eficácia clareadora antes e após as escovações simuladas. Os dados encontrados foram submetidos à Análise de Variância de 2 critérios (ANOVA-2) para medidas repetidas e teste de Tukey (ⲁ=0,05). Como resultados, houve diferença estatística em relação a cor (p<0,001) e tratamento (p<0,001), assim como, para a interação cor x tratamento (p<0,001). Ao avaliar a cor inicial e final de cada grupo individualmente, verificou-se diferença estatística significativa apenas para C10 (p<0,001). Os grupos WNGF (p=0,530) e TA (p=1) não apresentaram eficácia clareadora. Deste modo Concluiu-se que o dentifrício com o corante blue covarine não apresentou eficácia clareadora.
Incontinência urinária em mulheres praticantes de crossfit em Aracaju/SE
Authors: Leite Edna Giovanna Vieira , Prado Daniela Siqueira , Brandini Ana Beatriz Dantas
Published: December 2024

A incontinência urinaria (IU) é definida por qualquer perda involuntária de urina. Estudos demonstram que a prevalência nas mulheres jovens varia de 12% a 42%. A associação entre esportes de alto impacto no assoalho pélvico e perda de urina em mulheres jovens vem sendo verificada em algumas pesquisas. O Crossfit, modalidade esportiva em plena ascensão, vem sendo avaliado em virtude dos exercícios de alta intensidade dos treinos que cursam com aumento de pressão intra-abdominal. Objetivo: Estimar a prevalência e o impacto na qualidade de vida da incontinência urinária em atletas praticantes de crossfit e em um grupo controle. Metodologia: Estudo transversal analítico, com 104 mulheres na pré-menopausa, sendo 52 praticantes de crossfit e 52 sedentárias ou praticantes de atividades de baixo impacto. Aplicou-se o questionário International Consultationon Incontinence Questionnaire – Short Fonn a todas as participantes e o International Physical Activity Questionnaire para o grupo controle. A análise estatística foi realizada pelo BioEstat 5.0, t student para amostras independentes e correlação linear de Pearson para determinar a força das correlações. Resultados: Foi verificada uma prevalência de incontinência urinária global de 32,6%, sendo 15,3% em atletas e 17,3% no grupo controle. Dentre as incontinentes, a maioria das atletas e do grupo controle (82,7% em ambos os grupos) referiram não haver impacto significativo na qualidade de vida. Conclusão: A prevalência de incontinência urinária e o pequeno impacto na qualidade de vida foram similares entre atletas e grupo controle.
Osteomielite crônica não bacteriana em gemelares - relato de caso
Authors: Nobre Jéssica da Silva Machado , Robazzi Teresa Cristina Martins Vicente , Barros Leandra Chaves Silva
Published: December 2024

Introdução: A osteomileite crônica não bacteriana (OCNB) é uma doença rara no espectro das  doenças ósseas autoinflamatórias, que são caracterizadas pela inflamação asséptica do osso, não provocadas por condições autoimunes ou infecciosas, resultante da desregulação do sistema imune inato e do desequilíbrio das citocinas pró e anti-inflamatórias. Metodologia: Este estudo se trata de um relato de caso, realizado após consentimento da responsável pelas pacientes através da leitura e assinatura do Termo de Consentimento Livre e Esclarecido (TCLE), a partir de dados da consulta médica e de revisão em prontuário. Relato de caso: Este trabalho apresenta o caso clínico de duas pacientes, irmãs gêmeas univitelinas, 12 anos, com diagnóstico de Osteomielite Crônica Não Bacteriana, que desenvolveram a doença individualmente de maneira distinta. Discussão: O uso de diversas nomenclaturas para falar da Osteomielite Crônica Não Bacteriana e do seu variado espectro clínico e a falta de critérios diagnósticos são dois fatores que dificultam o diagnóstico da doença e resultam no atraso deste. O agrupamento familiar da OCNB esporádica e a associação com outras condições inflamatórias nesses pacientes e seus familiares de primeiro grau sugerem uma predisposição genética importante, ainda mal elucidada. Conclusão: A adoção de uma nomenclatura universal e a elaboração de critérios diagnósticos bem aceitos pela Comunidade Médica são necessários para melhor reconhecimento e diagnóstico da doença. Além disso, são necessários mais estudos para esclarecer o papel da genética e dos fatores ambientais na expressão e no desenvolvimento da  OCNB esporádica.
Correlação de marcadores inflamatórios com fatores prognósticos em transplantados cardíacos
Authors: Silva Marcus Vinícius de Paula da , Alves Antonielle Rodrigues Pereira , Vieira-Alves Ildernandes , Lemos Virginia Soares , Carmo Gabriel Assis Lopes do , Castilho Fábio Morato de , Gelape Cláudio Léo
Published: December 2024

Objetivo: Correlacionar a expressão das citocinas inflamatórias IL-1 β, TNF-α IL-6 e a contagem de macrófagos CD68 e neutrófilos CD66b no tecido da artéria pulmonar de transplantados cardíacos (TxC) com fatores de pior prognóstico. Métodos: Os níveis das interleucinas IL-6, IL-1β e TNF-α e de CD68 e neutrófilos CD66b, foram aferidos em fragmentos das artérias pulmonares de pacientes TxC com e sem fatores de mau prognóstico. Resultados: Não houve diferença estatisticamente significativa (p ≥ 0,05) entre os níveis dos marcadores inflamatórios aferidos nas artérias pulmonares dos TxC com ou sem fatores de mau prognóstico. Conclusão: Observamos que pacientes TxC, com fatores de pior prognóstico ou não, apresentaram níveis semelhantes de marcadores inflamatórios expressos nas artérias pulmonares. Tal fato sugere que o mau prognóstico no TxC está mais correlacionado com parâmetros clínicos propriamente ditos do que com a resposta inflamatória secundária a miocardiopatia associada.