bluuebunny/crossref_metadata_2025 huggingface dataset:
Smart Cooperative Energy Supply Strategy to Increase Reliability in Residential Stand-Alone Photovoltaic Systems
Authors: Roldán-Blay Carlos , Roldán-Porta Carlos , Quiles Eduardo , Escrivá-Escrivá Guillermo
Published: December 2021

In reliability studies of isolated energy supply systems for residential buildings, supply failures due to insufficient generation are generally analysed. Recent studies conclude that this kind of analysis makes it possible to optimally design the sizes of the elements of the generation system. However, in isolated communities or rural areas, it is common to find groups of dwellings in which micro-renewable sources, such as photovoltaic (PV) systems, can be installed. In this situation, the generation and storage of several houses can be considered as an interconnected system forming a cooperative microgrid (CoMG). This work analyses the benefits that sharing two autonomous installations can bring to each one, from the point of view of reliability. The method consists of the application of a random sequential Monte Carlo (SMC) simulation to the CoMG to evaluate the impact of a simple cooperative strategy on the reliability of the set. The study considers random failures in the generation systems. The results show that the reliability of the system increases when cooperation is allowed. Additionally, at the design stage, this allows more cost-effective solutions than single sizing with a similar level of reliability.
Enhanced Sensitivity of FeGa Thin-Film Coated SAW Current Sensor
Authors: Sun Yuan , Jia Yana , Zhang Yufeng , Cheng Lina , Liang Yong , Wang Wen
Published: December 2021

A surface acoustic wave (SAW) device is proposed for sensing current by employing the patterned FeGa thin film as the sensitive interface. The layered media structure of FeGa/SiO2/LiNbO3 was established to reveal the working principle of the sensors, and an SAW chip patterned by delay-line and operating at 150 MHz was fabricated photolithographically on 128° YX LiNbO3 substrate. The FeGa thin film with a larger magnetostrictive coefficient was sputtered onto the acoustic propagation path of the SAW chip to build the sensing device. The prepared device was connected into the differential oscillation loop to construct the current sensor. The FeGa thin film produces magnetostrictive strain and so-called ΔE effect at the magnetic field generated by the applied current, which modulates the SAW propagation velocity accordingly. The differential frequency signal was collected to characterize the measurand. Larger sensitivity of 37.9 kHz/A, low hysteresis error of 0.81%, excellent repeatability and stability were achieved in the experiments from the developed sensing device.
Special Issue “Current Concept and Emerging Treatments in Oral Diseases”
Authors: Tenore Gianluca
Published: December 2021

The attention of scientific research has grown in recent years on how to achieve more early and accurate diagnosis of oral diseases, and to carry out more efficient management of them [...]
Multiphysics Modeling and Material Selection Methods to Develop Optimal Piezoelectric Plate Actuators for Active Noise Cancellation
Authors: Molla Dessalew , Płaczek Marek , Wróbel Andrzej
Published: December 2021

The performance of a piezoelectric actuator for active noise cancellation depends primarily on the quality of the actuator material and its design approach, i.e., single-layer or multi-layer actuators, stacks, benders, or amplified actuators. In this paper, material selection and multiphysics modeling were performed to develop an optimal piezoelectric plate actuator for active noise cancellation. The material selection process was analyzed using two multi-criteria decision making (MCDM) approaches for material selection, i.e., figure of merit (FOM) for actuators and the technique for order of performance by similarity to ideal solution (TOPSIS). Of the 12 state-of-the-art piezoelectric actuator materials considered in this article, PMN–28% PT is the best material according to TOPSIS analysis, while PbIn12Nb12O324%−PbMg13Nb13O3−PbTiO3 (PIN24%-PMN-PT) is the best material according to FOM analysis. The ranking of state-of-the-art piezoelectric material categories for actuators according to the two analysis is consistent and the category of monocrystalline piezoelectric materials has the highest actuation performance. The multiphysics modeling was performed using ANSYS Mechanical using two different approaches: one using Ansys Parametric Design Language (APDL) command fragments, the other installing the PiezoAndMEMS ACT extension in ANSYS. Static structure, modal, and harmonic response analyses were performed to determine an optimal pair of piezoelectric plates to be used as an actuator for active noise cancellation. A pair of plates of the same materials, but of different dimensions turns out to be the optimal piezoelectric plate actuator for active noise reduction, according to the two multiphysics modeling methods.
Behavior Characteristics of Hazardous Gas and Scattering Coal Dust in Coal Storage Sheds
Authors: Cho Mok-Lyang , Ha Ji-Soo , Kim Tae-Kwon
Published: December 2021

This study has comprehensively analyzed the ventilation system of an indoor coal storage shed using computational fluid dynamics (CFD). In addition, the effects of the plan to improve the ventilation system were investigated by synthesizing the results. First, the velocity of inlet wind entering through the natural ventilation system was measured. Then, the concentration of carbon monoxide inside the coal storage shed was measured at the spontaneous combustion of coal. The boundary conditions were set using the measurement results. The characteristics of carbon monoxide concentration, ventilation volume, and behavior of scattering dust were analyzed. According to the CFD analysis results, the upper recirculation strength increased as the flow rate of external air increased. The flow-stagnation area occurred on the center wall. In particular, the concentration of carbon monoxide was high in the flow-stagnation area. When the inflow velocity was 2.0 m/s, a risk of 30 ppm or more occurred in the area near the second-floor workspace and central retaining wall. When ventilation dropped sharply, coal dust emissions decreased to 14.5%. Thus, ventilation must be secured by installing a natural ventilation system, in case spontaneous ignition occurs in many cells or the ventilation sharply decreases. Finally, in order to improve the ventilation system, the effect on the additional installation of natural ventilation and the use of mobile blowers was analyzed. Finally, in order to improve the ventilation system, the effect on the additional installation of natural ventilation and the use of mobile blowers was analyzed. As a result of the analysis, we concluded that using a mobile blower is more effective than a method of additionally installing a natural ventilation device. Carbon monoxide may be locally diluted, and ventilation volume additionally secured.
Visibility and Ceiling Nowcasting Using Artificial Intelligence Techniques for Aviation Applications
Authors: Cordeiro Fabricio Magalhães , França Gutemberg Borges , de Albuquerque Neto Francisco Leite , Gultepe Ismail
Published: December 2021

This work presents a novel approach for simulating visibility (Vis) and ceiling base height (Hc) in up to 1 h using several machine learning (ML) algorithms. Ten years of meteorological data at 15 min intervals for Santos Dumont airport (SDA), Rio de Janeiro, Brazil were used in the ML method training and testing process. In the investigation, several categorical and regressive algorithms were trained and tested, and the results were verified with observations. The forecast results reveal that the categorical methods produced satisfactory results only up to 15 min for visibility prediction with the probability of detection greater than 85%. On the other hand, the regressive methods were found to be more capable of generating an accurate prediction of Vis and Hc compared to categorical method up to 60 min. The forecast evaluation metrics for Vis and Hc had correlation coefficients of 0.99 ± 0.00 and 0.96 ± 0.00, with mean absolute errors of 324 ± 77 m, and 167 ± 21 m, respectively. Results suggested that ML methods can improve the prediction of Vis and Hc up to 1 h when accurate observations are used for the analysis.
Recent Development of Nickel-Rich and Cobalt-Free Cathode Materials for Lithium-Ion Batteries
Authors: Noerochim Lukman , Suwarno Suwarno , Idris Nurul Hayati , Dipojono Hermawan K.
Published: December 2021

The exponential growth in the production of electric vehicles requires an increasing supply of low-cost, high-performance lithium-ion batteries. The increased production of lithium-ion batteries raises concerns over the availability of raw materials, especially cobalt for batteries with nickel-rich cathodes, in which these constraints can impact the high price of cobalt. The reliance on cobalt in these cathodes is worrisome because it is a high-cost, rare material, with an unstable supply chain. This review describes the need and feasibility of developing cobalt-free high-nickel cathode materials for lithium-ion batteries. The new type of cathode material, LiNi1−x−yMnxAlyO2 promises a completely cobalt-free composition with almost the same electrochemical performance as that of the conventional high-nickel cathode. Therefore, this new type of cathode needs further research for its commercial applications.
Molecular Responses to High Hydrostatic Pressure in Eukaryotes: Genetic Insights from Studies on Saccharomyces cerevisiae
Authors: Abe Fumiyoshi
Published: December 2021

High hydrostatic pressure is common mechanical stress in nature and is also experienced by the human body. Organisms in the Challenger Deep of the Mariana Trench are habitually exposed to pressures up to 110 MPa. Human joints are intermittently exposed to hydrostatic pressures of 3–10 MPa. Pressures less than 50 MPa do not deform or kill the cells. However, high pressure can have various effects on the cell’s biological processes. Although Saccharomyces cerevisiae is not a deep-sea piezophile, it can be used to elucidate the molecular mechanism underlying the cell’s responses to high pressures by applying basic knowledge of the effects of pressure on industrial processes involving microorganisms. We have explored the genes associated with the growth of S. cerevisiae under high pressure by employing functional genomic strategies and transcriptomics analysis and indicated a strong association between high-pressure signaling and the cell’s response to nutrient availability. This review summarizes the occurrence and significance of high-pressure effects on complex metabolic and genetic networks in eukaryotic cells and how the cell responds to increasing pressure by particularly focusing on the physiology of S. cerevisiae at the molecular level. Mechanosensation in humans has also been discussed.
Comparative Toxicological Evaluation of Tattoo Inks on Two Model Organisms
Authors: Carotenuto Rosa , Fogliano Chiara , Rienzi Mariangela , Siciliano Antonietta , Salvatore Maria Michela , De Tommaso Gaetano , Benvenuto Giovanna , Galdiero Emilia , Guida Marco
Published: December 2021

Tattooing is a technique that introduces colored substances under the skin in order to color it permanently. Decomposition products of tattoo pigments produce numerous damages for the skin and other organs. We studied the effects of a commercial red ink tattoo, PR170, on Xenopus laevis embryos and Daphnia magna nauplii using concentrations of 10, 20, and 40 mg/L. For Xenopus, we applied the FETAX protocol analyzing survival, malformations, growth, heart rate, and the expression of genes involved in the development. In D. magna, we evaluated the toxicity with an immobilization test. Moreover, we investigated the production of ROS, antioxidant enzymes, and the expression of the ATP-binding cassette in both models. Our results indicate that PR170 pigment has nanoparticle dimensions, modifies the survival and the ATP-binding cassette activity, and induces oxidative stress that probably produces the observed effects in both models. Deformed embryos were observed in Xenopus, probably due to the modification of expression of genes involved in development. The expression of pro-inflammatory cytokines was also modified in this amphibian. We think that these effects are due to the accumulation of PR170 and, in particular, to the presence of the azoic group in the chemical structure of this pigment. Further studies needed to better understand the effects of commercial tattoo inks.
Insights into the Binding of Receptor-Binding Domain (RBD) of SARS-CoV-2 Wild Type and B.1.620 Variant with hACE2 Using Molecular Docking and Simulation Approaches
Authors: Muhseen Ziyad Tariq , Kadhim Salim , Yahiya Yahiya Ibrahim , Alatawi Eid A. , Aba Alkhayl Faris F. , Almatroudi Ahmad
Published: December 2021

Recently, a new variant, B.1620, with mutations (S477N-E484K) in the spike protein’s receptor-binding domain (RBD) has been reported in Europe. In order to design therapeutic strategies suitable for B.1.620, further studies are required. A detailed investigation of the structural features and variations caused by these substitutions, that is, a molecular level investigation, is essential to uncover the role of these changes. To determine whether and how the binding affinity of ACE2–RBD is affected, we used protein–protein docking and all-atom simulation approaches. Our analysis revealed that B.1.620 binds more strongly than the wild type and alters the hydrogen bonding network. The docking score for the wild type was reported to be −122.6 +/− 0.7 kcal/mol, while for B.1.620, the docking score was −124.9 +/− 3.8 kcal/mol. A comparative binding investigation showed that the wild-type complex has 11 hydrogen bonds and one salt bridge, while the B.1.620 complex has 14 hydrogen bonds and one salt bridge, among which most of the interactions are preserved between the wild type and B.1.620. A dynamic analysis of the two complexes revealed stable dynamics, which corroborated the global stability trend, compactness, and flexibility of the three essential loops, providing a better conformational optimization opportunity and binding. Furthermore, binding free energy revealed that the wild type had a total binding energy of −51.14 kcal/mol, while for B.1.628, the total binding energy was −68.25 kcal/mol. The current findings based on protein complex modeling and bio-simulation methods revealed the atomic features of the B.1.620 variant harboring S477N and E484K mutations in the RBD and the basis for infectivity. In conclusion, the current study presents distinguishing features of B.1.620, which can be used to design structure-based drugs against the B.1.620 variant.
(−)-Epigallocatechin-3-Gallate Diminishes Intra-and Extracellular Amyloid-Induced Cytotoxic Effects on Cholinergic-like Neurons from Familial Alzheimer’s Disease PSEN1 E280A
Authors: Soto-Mercado Viviana , Mendivil-Perez Miguel , Velez-Pardo Carlos , Jimenez-Del-Rio Marlene
Published: December 2021

Alzheimer’s disease (AD) is a complex neurodegenerative disease characterized by functional disruption, death of cholinergic neurons (ChNs) because of intracellular and extracellular Aβ aggregates, and hyperphosphorylation of protein TAU (p-TAU). To date, there are no efficient therapies against AD. Therefore, new therapies for its treatment are in need. The goal of this investigation was to evaluate the effect of the polyphenol epigallocatechin-3-gallate (EGCG) on cholinergic-like neurons (ChLNs) bearing the mutation E280A in PRESENILIN 1 (PSEN1 E280A). To this aim, wild-type (WT) and PSEN1 E280A ChLNs were exposed to EGCG (5–50 μM) for 4 days. Untreated or treated neurons were assessed for biochemical and functional analysis. We found that EGCG (50 μM) significantly inhibited the aggregation of (i)sAPPβf, blocked p-TAU, increased ∆Ψm, decreased oxidation of DJ-1 at residue Cys106-SH, and inhibited the activation of transcription factor c-JUN and P53, PUMA, and CASPASE-3 in mutant ChLNs compared to WT. Although EGCG did not reduce (e)Aβ42, the polyphenol reversed Ca2+ influx dysregulation as a response to acetylcholine (ACh) stimuli in PSEN1 E280A ChLNs, inhibited the activation of transcription factor NF-κB, and reduced the secretion of pro-inflammatory IL-6 in wild-type astrocyte-like cells (ALCs) when exposed to mutant ChLNs culture supernatant. Taken together, our findings suggest that the EGCG might be a promising therapeutic approach for the treatment of FAD.
Probing the Influence of Single-Site Mutations in the Central Cross-β Region of Amyloid β (1–40) Peptides
Authors: Fritzsch Jacob , Korn Alexander , Surendran Dayana , Krueger Martin , Scheidt Holger A. , Mote Kaustubh R. , Madhu Perunthiruthy K. , Maiti Sudipta , Huster Daniel
Published: December 2021

Amyloid β (Aβ) is a peptide known to form amyloid fibrils in the brain of patients suffering from Alzheimer’s disease. A complete mechanistic understanding how Aβ peptides form neurotoxic assemblies and how they kill neurons has not yet been achieved. Previous analysis of various Aβ40 mutants could reveal the significant importance of the hydrophobic contact between the residues Phe19 and Leu34 for cell toxicity. For some mutations at Phe19, toxicity was completely abolished. In the current study, we assessed if perturbations introduced by mutations in the direct proximity of the Phe19/Leu34 contact would have similar relevance for the fibrillation kinetics, structure, dynamics and toxicity of the Aβ assemblies. To this end, we rationally modified positions Phe20 or Gly33. A small library of Aβ40 peptides with Phe20 mutated to Lys, Tyr or the non-proteinogenic cyclohexylalanine (Cha) or Gly33 mutated to Ala was synthesized. We used electron microscopy, circular dichroism, X-ray diffraction, solid-state NMR spectroscopy, ThT fluorescence and MTT cell toxicity assays to comprehensively investigate the physicochemical properties of the Aβ fibrils formed by the modified peptides as well as toxicity to a neuronal cell line. Single mutations of either Phe20 or Gly33 led to relatively drastic alterations in the Aβ fibrillation kinetics but left the global, as well as the local structure, of the fibrils largely unchanged. Furthermore, the introduced perturbations caused a severe decrease or loss of cell toxicity compared to wildtype Aβ40. We suggest that perturbations at position Phe20 and Gly33 affect the fibrillation pathway of Aβ40 and, thereby, influence the especially toxic oligomeric species manifesting so that the region around the Phe19/Leu34 hydrophobic contact provides a promising site for the design of small molecules interfering with the Aβ fibrillation pathway.
The Expression and Activity of Rhodanese, 3-Mercaptopyruvate Sulfurtransferase, Cystathionine γ-Lyase in the Most Frequently Chosen Cellular Research Models
Authors: Kaczor-Kamińska Marta , Kaminski Kamil , Wróbel Maria
Published: December 2021

This paper provides information concerning the activity and expression levels of three sulfurtransferases (STRs): rhodanese (TST, EC: 2.8.1.1), 3-mercaptopyruvate sulfurtransferase (MPST, EC: 2.8.1.2) and cystathionine γ-lyase (CTH, EC: 4.4.1.1) in various cell lines. Since very limited data are available in the scientific literature on this subject, the available data are included in this paper. These shortages often force the researchers to carry out their own screening tests that allow them to choose an appropriate model for their further studies. This work supplements the existing deficiencies in this area and presents the activity and expression of STRs in the eight most frequently chosen cell lines: the mouse mammary gland cell line (NMuNG, ATCC: CRL-1636), mouse mammary gland tumor (4T1, ATCC: CRL-2539), mouse fibroblast (MEF, ATCC: SCRC-1008), mouse melanoma (B16-F1, ATCC: CRL-6323), human colorectal adenocarcinoma (Caco-2, ATCC: HTB-37), human embryonic kidney (HEK-293, ATCC: CRL-1573), human osteosarcoma (MG-63, ATCC: CRL-1427) and rat myocardium (H9c2, ATCC: CRL-1446). Changes in STRs activity are directly related to the bioavailability of cysteine and the sulfane sulfur level, and thus the present authors also measured these parameters, as well as the level of glutathione (its reduced (GSH) and oxidized (GSSG) form) and the [GSH]/[GSSG] ratio that determines the antioxidant capacity of the cells. STRs demonstrate diverse functionality and clinical relevance; therefore, we also performed an analysis of genetic variation of STRs genes that revealed a large number of polymorphisms. Although STRs still provide challenges in several fields, responding to them could not only improve the understanding of various diseases, but may also provide a way to treat them.
N-Acetylcysteine in Mechanically Ventilated Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome: The Effect of Intravenous Dose on Oxidative Damage and Inflammation
Authors: Kolomaznik Maros , Mikolka Pavol , Hanusrichterova Juliana , Kosutova Petra , Matasova Katarina , Mokra Daniela , Calkovska Andrea
Published: December 2021

Treatment of acute respiratory distress syndrome (ARDS) is challenging due to its multifactorial aetiology. The benefit of antioxidant therapy was not consistently demonstrated by previous studies. We evaluated the effect of two different doses of intravenous (i.v.) N-acetylcysteine (NAC) on oxidative stress, inflammation and lung functions in the animal model of severe LPS-induced lung injury requiring mechanical ventilation. Adult Wistar rats with LPS (500 μg/kg; 2.2 mL/kg) were treated with i.v. NAC 10 mg/kg (NAC10) or 20 mg/kg (NAC20). Controls received saline. Lung functions, lung oedema, total white blood cell (WBC) count and neutrophils count in blood and bronchoalveolar lavage fluid, and tissue damage in homogenized lung were evaluated. NAC significantly improved ventilatory parameters and oxygenation, reduced lung oedema, WBC migration and alleviated oxidative stress and inflammation. NAC20 in comparison to NAC10 was more effective in reduction of oxidative damage of lipids and proteins, and inflammation almost to the baseline. In conclusion, LPS-instilled and mechanically ventilated rats may be a suitable model of ARDS to test the treatment effects at organ, systemic, cellular and molecular levels. The results together with literary data support the potential of NAC in ARDS.
Rewarding Social Interaction in Rats Increases CaMKII in the Nucleus Accumbens
Authors: Amaral Inês M. , Scheffauer Laura , Langeder Angelika B. , Hofer Alex , El Rawas Rana
Published: December 2021

Calcium/calmodulin-dependent protein kinase II (CaMKII) is known to be involved in the sensitized locomotor responses and drug-seeking behavior to psychostimulants. However, little is known about the contribution of CaMKII signaling in the nucleus accumbens (NAc) in natural rewards such as social interaction. The present experiments explored the implication of CaMKII signaling in drug versus natural reward. In the NAc of rats expressing cocaine or social interaction conditioned place preference (CPP), αCaMKII activation was induced in those expressing social interaction but not cocaine CPP. In order to investigate the role of NAc CaMKII in the expression of reward-related learning of drug versus non-drug stimuli, we inhibited CaMKII through an infusion of KN-93, a CaMKII inhibitor, directly into the NAc shell or core, before the CPP test in a concurrent paradigm in which social interaction was made available in the compartment alternative to the one associated with cocaine during conditioning. Whereas vehicle infusions led to equal preference to both stimuli, inhibition of CaMKII by a KN-93 infusion before the CPP test in the shell but not the core of the NAc shifted the rats’ preference toward the cocaine-associated compartment. Altogether, these results suggest that social interaction reward engages CaMKII in the NAc.
Lipid Droplets, Phospholipase A2, Arachidonic Acid, and Atherosclerosis
Authors: Bermúdez Miguel A. , Balboa María A. , Balsinde Jesús
Published: December 2021

Lipid droplets, classically regarded as static storage organelles, are currently considered as dynamic structures involved in key processes of lipid metabolism, cellular homeostasis and signaling. Studies on the inflammatory state of atherosclerotic plaques suggest that circulating monocytes interact with products released by endothelial cells and may acquire a foamy phenotype before crossing the endothelial barrier and differentiating into macrophages. One such compound released in significant amounts into the bloodstream is arachidonic acid, the common precursor of eicosanoids, and a potent inducer of neutral lipid synthesis and lipid droplet formation in circulating monocytes. Members of the family of phospholipase A2, which hydrolyze the fatty acid present at the sn-2 position of phospholipids, have recently emerged as key controllers of lipid droplet homeostasis, regulating their formation and the availability of fatty acids for lipid mediator production. In this paper we discuss recent findings related to lipid droplet dynamics in immune cells and the ways these organelles are involved in regulating arachidonic acid availability and metabolism in the context of atherosclerosis.
Efficacy and Synergy of Small Molecule Inhibitors Targeting FLT3-ITD+ Acute Myeloid Leukemia
Authors: Bregante Javier , Schönbichler Anna , Pölöske Daniel , Degenfeld-Schonburg Lina , Monzó Contreras Garazi , Hadzijusufovic Emir , de Araujo Elvin D. , Valent Peter , Moriggl Richard , Orlova Anna
Published: December 2021

Constitutive activation of FLT3 by ITD mutations is one of the most common genetic aberrations in AML, present in ~1/3 of cases. Patients harboring FLT3-ITD display worse clinical outcomes. The integration and advancement of FLT3 TKI in AML treatment provided significant therapeutic improvement. However, due to the emergence of resistance mechanisms, FLT3-ITD+ AML remains a clinical challenge. We performed an unbiased drug screen to identify 18 compounds as particularly efficacious against FLT3-ITD+ AML. Among these, we characterized two investigational compounds, WS6 and ispinesib, and two approved drugs, ponatinib and cabozantinib, in depth. We found that WS6, although not yet investigated in oncology, shows a similar mechanism and potency as ponatinib and cabozantinib. Interestingly, ispinesib and cabozantinib prevent activation of AXL, a key driver and mechanism of drug resistance in FLT3-ITD+ AML patients. We further investigated synergies between the selected compounds and found that combination treatment with ispinesib and cabozantinib or ponatinib shows high synergy in FLT3-ITD+ AML cell lines and patient samples. Together, we suggest WS6, ispinesib, ponatinib and cabozantinib as novel options for targeting FLT3-ITD+ AML. Whether combinatorial tyrosine kinase and kinesin spindle blockade is effective in eradicating neoplastic (stem) cells in FLT3-ITD+ AML remains to be determined in clinical trials.
Adjuvant Hysterectomy for Cervical Cancer Patients Treated with Chemoradiation Therapy: A Systematic Review on the Pathology-Proven Residual Disease Rate
Authors: van Kol Kim , Ebisch Renée , Piek Jurgen , Beugeling Maaike , Vergeldt Tineke , Bekkers Ruud
Published: December 2021

Objective: To determine the incidence of pathology-proven residual disease in adjuvant hysterectomy specimens in patients with cervical cancer, treated with chemoradiation therapy. Secondly, to assess a possible association for pathology-proven residual disease regarding the time between chemoradiation therapy and adjuvant hysterectomy. Additionally, the survival rate and complication rate were assessed. Methods: PubMed, EMBASE, and the Cochrane database were searched from inception up to 8 March 2021. Results: Of the 4601 screened articles, eleven studies were included. A total of 1205 patients were treated with chemoradiation therapy and adjuvant hysterectomy, ranging from three to twelve weeks after chemoradiation therapy. A total of 411 out of 1205 patients (34%) had pathology-proven residual disease in the adjuvant hysterectomy specimen. There was no association found in the time between chemoradiation therapy and adjuvant hysterectomy. Follow-up ranged from 2.4 to 245 months, during which 270 patients (22%) relapsed, and 298 patients (27%) were deceased. A total of 202 (35%) complications were registered in 578 patients. Conclusion: there is no association found in the time between chemoradiation therapy and residual disease on adjuvant hysterectomy specimens. The survival rates after chemoradiation therapy and adjuvant hysterectomy are suboptimal, while the risk of complications after adjuvant hysterectomy is high.
Cognitive Decline following Radiotherapy of Head and Neck Cancer: Systematic Review and Meta-Analysis of MRI Correlates
Authors: Voon Noor Shatirah , Abdul Manan Hanani , Yahya Noorazrul
Published: December 2021

Radiotherapy for head and neck cancers exposes small parts of the brain to radiation, resulting in radiation-induced changes in cerebral tissue. In this review, we determine the correlation between cognitive deterioration in patients with head and neck cancer after radiotherapy and magnetic resonance imaging (MRI) changes. Systematic searches were performed in PubMed, Scopus, and Cochrane databases in February 2021. Studies of head and neck cancer patients treated with radiotherapy and periodical cognitive and MRI assessments were included. Meta-analysis was performed to analyse the correlation of Montreal Cognitive Assessment (MoCA) scores to MRI structural and functional changes. Seven studies with a total of 404 subjects (irradiated head and neck patients, n = 344; healthy control, n = 60) were included. Most studies showed the significance of MRI in detecting microstructural and functional changes in association with neurocognitive function. The changes were seen in various brain areas, predominantly the temporal region, which also shows dose dependency (6/7 studies). An effect size (r = 0.43, p < 0.001) was reported on the correlation of MoCA scores to MRI structural and functional changes with significant correlations shown among patients treated with head and neck radiotherapy. However, the effect size appears modest.
Malignant Vascular Tumors of the Head and Neck—Which Type of Therapy Works Best?
Authors: Wiegand Susanne , Dietz Andreas , Wichmann Gunnar
Published: December 2021

Malignant vascular tumors of the head and neck are rare neoplasms with variable clinical presentation, wide age distribution, and variable clinical courses. The heterogeneous presentation of angiosarcomas and epithelioid hemangioendothelioma often leads to misdiagnosis and unsuitable treatment. While risk factors for angiosarcomas are previous radiation, chronic lymphedema, and exposure to arsenic, thorium oxide, or vinyl chloride, there are only limited and retrospective data available on prognostic factors in EHE. In both angiosarcomas and EHE, surgery is the mainstay of treatment. There is limited evidence regarding the role of radiotherapy in EHE, although EHE is considered relatively radiosensitive. In angiosarcomas, adjuvant radiotherapy is recommended according to retrospective case series. A standard medical therapy for metastasized malignant vascular tumors is lacking. Chemotherapy, which is effective in angiosarcoma, is mostly ineffective in EHE. Targeted therapy, antiangiogenetic drugs and immunotherapy have been studied as new treatment options. The goal of this review is to summarize the current data regarding malignant vascular tumors along with their diagnosis and management.
Acute and Long-Term Outcomes of ST-Elevation Myocardial Infarction in Cancer Patients, a ‘Real World’ Analysis with 175,000 Patients
Authors: Lange Stefan A. , Feld Jannik , Kühnemund Leonie , Köppe Jeanette , Makowski Lena , Engelbertz Christiane M. , Gerß Joachim , Dröge Patrik , Ruhnke Thomas , Günster Christian , Freisinger Eva , Reinecke Holger
Published: December 2021

Background: Acute myocardial infarction (AMI) and cancer are common and serious diseases. As the prognosis and treatment of both diseases has improved, more cancer patients will suffer an AMI. Unfortunately, data on these “double hit” patients is scarce. Methods: From the largest public German health insurance, anonymized data of all patients with pre-existing cancer who were hospitalized due to ST-elevation MI (STEMI) between 2010 and 2017 were analyzed and followed-up until 2018. Results: Of 175,262 STEMI patients, 27,213 had pre-existing cancer (15.5%). Most frequent were skin (24.9%), prostate (17.0%), colon (11.0%), breast (10.9%), urinary tract (10.6%), and lung cancer (5.2%). STEMI patients with malignancies were older and presented more often with coronary three-vessel disease, atrial arrhythmias, chronic kidney disease, chronic heart failure, cerebrovascular and peripheral artery disease (PAD, each p < 0.001). They showed more often previous AMI, percutaneous coronary interventions (PCI), cardiac surgery, and stroke (all p < 0.001). Acute PCIs were applied between 2 and 6% less frequently compared to those without cancer. In-hospital adverse events occurred more frequently in cancer. Eight-year survival was 57.3% (95% CI 57.0–57.7%) without cancer and ranged between 41.2% and 19.2% in distinct cancer types. Multivariable Cox regression for all-cause mortality found, e.g., lung cancer (HR 2.04), PAD stage 4–6 (HR 1.78), metastasis (HR 1.72), and previous stroke (HR 1.44) to have the strongest impact (all p < 0.001). Conclusion: In this large “real world” data, prognosis after STEMI in cancer patients was markedly reduced but differed widely between cancer types. Of note, no withholding of interventional treatments in cancer patients could be observed.
Autothermal Reforming of Acetic Acid to Hydrogen and Syngas on Ni and Rh Catalysts
Authors: Tande Lifita N. , Resendiz-Mora Erik , Dupont Valerie , Twigg Martyn V.
Published: December 2021

The autothermal reforming (ATR) of acetic acid (HAc) as a model bio-oil compound is examined via bench scale experiments and equilibrium modelling to produce hydrogen and syngas. This study compares the performance of nickel (Ni-Al, Ni-CaAl) vs. rhodium (Rh-Al) for particulate packed bed (PPB), and of Rh-Al in PPB vs. Rh with and without Ceria for honeycomb monolith (‘M’) catalysts (R-M and RC-M). All PPB and M catalysts used Al2O3 as main support or washcoat, and when not pre-reduced, exhibited good performance with more than 90% of the HAc converted to C1-gases. The maximum H2 yield (6.5 wt.% of feed HAc) was obtained with both the Rh-Al and Ni-CaAl catalysts used in PPB, compared to the equilibrium limit of 7.2 wt.%, although carbon deposition from Ni-CaAl at 13.9 mg gcat−1 h−1 was significantly larger than Rh-Al’s (5.5 mg gcat−1 h−1); close to maximum H2 yields of 6.2 and 6.3 wt.% were obtained for R-M and RC-M respectively. The overall better performance of the Ni-CaAl catalyst over that of the Ni-Al was attributed to the added CaO reducing the acidity of the Al2O3 support, which provided a superior resistance to persistent coke formation. Unlike Rh-Al, the R-M and RC-M exhibited low steam conversions to H2 and CH4, evidencing little activity in water gas shift and methanation. However, the monolith catalysts showed no significant loss of activity, unlike Ni-Al. Both catalytic PPB (small reactor volumes) and monolith structures (ease of flow, strength, and stability) offer different advantages, thus Rh and Ni catalysts with new supports and structures combining these advantages for their suitability to the scale of local biomass resources could help the future sustainable use of biomasses and their bio-oils as storage friendly and energy dense sources of green hydrogen.
Complexes of Transition Metals with Petroleum Porphyrin Ligands: Preparation and Evaluation of Catalytic Ability
Authors: Tazeev Damir , Musin Lenar , Mironov Nikolay , Milordov Dmitry , Tazeeva Elvira , Yakubova Svetlana , Yakubov Makhmut
Published: December 2021

In the present work, the first data on the catalytic activity of d-metal complexes of petroleum porphyrins obtained via two-stage re-metallization (acid demetallization with subsequent metalation) of high-purity petroleum vanadyl porphyrins are presented. During acid demetallization of petroleum vanadyl porphyrins, the highest yield (49%) and spectral purity of free petroporphyrin bases were achieved with concentrated sulfuric acid and a diluted solution of vanadyl porphyrins in chloroform. In the series of divalent cations of Mn, Fe, Co, Ni, Cu, and Zn, only the last four metals are complexed with demetallated petroporphyrins without significant changes in their component composition, whereas the interaction with Mn and Fe cations causes an evident structural transformation or even full degradation of petroporphyrin macrocycles, respectively. The composition and spectral purity of petroleum porphyrin-containing reactants and products were analyzed by FT-IR, UV-Vis, NMR, and MALDI-TOF mass spectroscopic methods. The obtained petroporphyrin-based d-metal complexes were assayed by the reaction of 2-mercaptoethanol oxidative dimerization, in which the copper porphyrins exhibited the highest catalytic activity.
Reprogramming Human Adult Fibroblasts into GABAergic Interneurons
Authors: Bruzelius Andreas , Kidnapillai Srisaiyini , Drouin-Ouellet Janelle , Stoker Tom , Barker Roger A. , Rylander Ottosson Daniella
Published: December 2021

Direct reprogramming is an appealing strategy to generate neurons from a somatic cell by forced expression of transcription factors. The generated neurons can be used for both cell replacement strategies and disease modelling. Using this technique, previous studies have shown that γ-aminobutyric acid (GABA) expressing interneurons can be generated from different cell sources, such as glia cells or fetal fibroblasts. Nevertheless, the generation of neurons from adult human fibroblasts, an easily accessible cell source to obtain patient-derived neurons, has proved to be challenging due to the intrinsic blockade of neuronal commitment. In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1 Silencing Transcription Factor (REST) inhibition together with a combination of GABAergic fate determinants to convert human adult skin fibroblasts into GABAergic neurons. Our results show a successful conversion in 25 days with upregulation of neuronal gene and protein expression levels. Moreover, we identified specific gene combinations that converted fibroblasts into neurons of a GABAergic interneuronal fate. Despite the well-known difficulty in converting adult fibroblasts into functional neurons in vitro, we could detect functional maturation in the induced neurons. GABAergic interneurons have relevance for cognitive impairments and brain disorders, such as Alzheimer’s and Parkinson’s diseases, epilepsy, schizophrenia and autism spectrum disorders.
T-Bet Deficiency Attenuates Bile Duct Injury in Experimental Biliary Atresia
Authors: Mohanty Sujit K. , Donnelly Bryan , Temple Haley , Bondoc Alexander , McNeal Monica , Tiao Greg
Published: December 2021

Biliary atresia (BA) is an obstructive neonatal cholangiopathy leading to liver cirrhosis and end stage liver disease. A Kasai portoenterostomy may restore biliary drainage, but most patients ultimately require liver transplantation for survival. At diagnosis, immune cells within the liver of patients with BA demonstrate a T-helper 1 (Th1) inflammatory profile similar to rhesus rotavirus (RRV)-infected mice livers developing BA. The transcription factor Tbx21 (T-bet) is essential for induction of a Th1 immune response in both the adaptive and innate immune system. Here we used animals with targeted deletion of the T-bet gene to determine its role in the progression of BA. Infection of newborn T-bet knockout (KO) pups with RRV resulted in a decreased Th1 inflammatory chemokine/cytokine profile when compared to infected wild-type mice. Analysis of the mononuclear cells profile from T-bet KO mice revealed both a significant decrease in the total number of CD3, CD4, and CD8 T cells and their effector molecules granzyme A, perforin, and FasL. Even though the percentage of T-bet KO mice displaying symptoms of an obstructive cholangiopathy and overall mortality rate was not different compared to wild-type mice, the extrahepatic bile ducts of T-bet KO mice remained patent.
Development of Stem Cell-Derived Immune Cells for Off-the-Shelf Cancer Immunotherapies
Authors: Li Yan-Ruide , Dunn Zachary Spencer , Zhou Yang , Lee Derek , Yang Lili
Published: December 2021

Cell-based cancer immunotherapy has revolutionized the treatment of hematological malignancies. Specifically, autologous chimeric antigen receptor-engineered T (CAR-T) cell therapies have received approvals for treating leukemias, lymphomas, and multiple myeloma following unprecedented clinical response rates. A critical barrier to the widespread usage of current CAR-T cell products is their autologous nature, which renders these cellular products patient-selective, costly, and challenging to manufacture. Allogeneic cell products can be scalable and readily administrable but face critical concerns of graft-versus-host disease (GvHD), a life-threatening adverse event in which therapeutic cells attack host tissues, and allorejection, in which host immune cells eliminate therapeutic cells, thereby limiting their antitumor efficacy. In this review, we discuss recent advances in developing stem cell-engineered allogeneic cell therapies that aim to overcome the limitations of current autologous and allogeneic cell therapies, with a special focus on stem cell-engineered conventional αβ T cells, unconventional T (iNKT, MAIT, and γδ T) cells, and natural killer (NK) cells.
Structural and Electrochemical Studies of Cobalt(II) and Nickel(II) Coordination Polymers with 6-Oxonicotinate and 4,4′-Bipyridine
Authors: Škugor Rončević Ivana , Vladislavić Nives , Chatterjee Nabanita , Sokol Vesna , Oliver Clive L. , Kukovec Boris-Marko
Published: December 2021

The 6-oxonicotinate (6-Onic) salts of a one-dimensional cationic cobalt(II) or nickel(II) coordination polymers with 4,4′-bipyridine (4,4′-bpy), namely {[Co(4,4′-bpy)(H2O)4](6-Onic)2·2H2O}n (1) and {[Ni(4,4′-bpy)(H2O)4](6-Onic)2·2H2O}n (2), were prepared hydrothermally by reactions of cobalt(II) nitrate hexahydrate or nickel(II) nitrate hexahydrate, respectively, 6-hydroxynicotinic acid and 4,4′-bipyridine in a mixture of ethanol and water. In the hydrogen-bonded frameworks of 1 and 2, the one-dimensional polymeric chains of {[M(4,4′-bpy)(H2O)4]2+}n (M = Co, Ni), the 6-oxonicotinate anions and the lattice water molecules were assembled via strong intermolecular O–H···O and N–H···O hydrogen bonds and π–π interactions, leading to the formation of the representative hydrogen-bond ring motifs: trimeric R23(10) motif, the centrosymmetric tetrameric R24(8) and R24(12) motifs and the pentameric R45(12) motif. The isostructural coordination polymers 1 and 2 exhibited a different electrochemical behavior, as observed by cyclic voltammetry, which can be attributed to the nature of the metal ions (cobalt(II) vs. nickel(II)).
Detection of Monilia Contamination in Plum and Plum Juice with NIR Spectroscopy and Electronic Tongue
Authors: Vitalis Flora , Tjandra Nugraha David , Aouadi Balkis , Aguinaga Bósquez Juan Pablo , Bodor Zsanett , Zaukuu John-Lewis Zinia , Kocsis Tamás , Zsom-Muha Viktória , Gillay Zoltan , Kovacs Zoltan
Published: December 2021

Plums are one of the commercially important stone fruits that are available on the market in both fresh and processed form and the most sought-after products are prunes, cans, jams, and juices. Maturity, harvest, and post-harvest technologies fundamentally determine the relatively short shelf life of plums which is often threatened by Monilinia spp. Causing brown rot worldwide. The aim of the present research was to use advanced analytical techniques, such as hand-held near infrared spectroscopy (NIRS) and electronic tongue (e-tongue) to detect M. fructigena fungal infection on plums and quantify this fungal contamination in raw plum juices. For this purpose, plums were inoculated with fungal mycelia in different ways (control, intact, and through injury) and stored under different conditions (5 °C, and 24 °C) for eight days. The results obtained with the two instruments were analyzed with chemometric methods, such as linear discriminant analysis (LDA) and partial least squares regression (PLSR). The NIRS-based method proved successful when detectability before the appearance of visible signs of the infection was studied. E-tongue was able to detect and quantify the concentration of juice derived from plum developed with M. fructigena with RMSECV lower than 5% w/w. Overall, the two methods proved to be suitable for discriminating between the treatment groups, however, the classification accuracy was higher for samples stored at 24 °C. The research results show both NIRS and e-tongue are beneficial methods to reduce food waste by providing rapid determination of fruit quality.
Lorenz Atmospheric Energy Cycle in Climatic Projections
Authors: Michaelides Silas
Published: December 2021

The aim of this study is to investigate whether different Representative Concentration Pathways (RCPs), as they are determined in the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC), lead to different regimes in the energetics components of the Lorenz energy cycle. The four energy forms on which this investigation is based are the zonal and eddy components of the available potential and kinetic energies. The corresponding transformations between these forms of energy are also studied. RCPs are time-dependent, consistent scenarios of concentrations of radiatively active gases and particles. In the present study, four RCPs are explored, namely, rcp26, rcp45, rcp60, rcp85; these represent projections (for the future period 2006–2100) that result in radiative forcing of approximately 2.6, 4.5, 6.0 and 8.5 Wm−2 at year 2100, respectively, relative to pre-industrial conditions. The results are presented in terms of time projections of the energetics components from 2020 to 2100 and show that the different RCPs yield diverse energetics regimes, consequently impacting the Lorenz energy cycle. In this respect, projections under different RCPs of the Lorenz energy cycle are presented.
Verification and Bias Adjustment of ECMWF SEAS5 Seasonal Forecasts over Europe for Climate Service Applications
Authors: Crespi Alice , Petitta Marcello , Marson Paola , Viel Christian , Grigis Lucas
Published: December 2021

This work discusses the ability of a bias-adjustment method using empirical quantile mapping to improve the skills of seasonal forecasts over Europe for three key climate variables, i.e., temperature, precipitation and wind speed. In particular, the suitability of the approach to be integrated in climate services and to provide tailored predictions for local applications was evaluated. The workflow was defined in order to allow a flexible implementation and applicability while providing accurate results. The scheme adjusted monthly quantities from the seasonal forecasting system SEAS5 of the European Centre for Medium-Range Forecasts (ECMWF) by using ERA5 reanalysis as reference. Raw and adjusted forecasts were verified through several metrics analyzing different aspects of forecast skills. The applied method reduced model biases for all variables and seasons even though more limited improvements were obtained for precipitation. In order to further assess the benefits and limitations of the procedure, the results were compared with those obtained by the ADAMONT method, which calibrates daily quantities by empirical quantile mapping conditioned by weather regimes. The comparable performances demonstrated the overall suitability of the proposed method to provide end users with calibrated predictions of monthly and seasonal quantities.