bluuebunny/crossref_metadata_2025 huggingface dataset:
Causal Effects between Gut Microbiome and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: A Two-Sample Mendelian Randomization Study
Authors: He Gang , Cao Yu , Ma Honghao , Guo Siran , Xu Wangzi , Wang Dai , Chen Yongquan , Wang Houzhao
Published: July 2023

Background

Evidence from previous studies have implicated an important association between gut microbiota (GM) and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS), but whether there is a definite causal relationship between GM and ME/CFS has not been elucidated.

Method

This study obtained instrumental variables of 211 GM taxa from the Genome Wide Association Study (GWAS), and mendelian randomization (MR) study was carried out to assess the effect of gut microbiota on ME/CFS risk from UK Biobank GWAS (2076 ME/CFS cases and 460,857 controls). Inverse variance weighted (IVW) was the primary method to analyze causality in this study, and a series of sensitivity analyses was performed to validate the robustness of the results.

Results

The inverse variance weighted (IVW) method indicated that genus Paraprevotella (OR:1.001, 95%CI:1.000–1.003, value of p < 0.05) and Ruminococca- ceae_UCG_014 (OR 1.003, 95% CI 1.000 to 1.005, value of p < 0.05) were positively associated with ME/CFS risk. Results from the weighted median method supported genus Paraprevotella (OR 1.003, 95% CI 1.000 to 1.005, value of p < 0.05) as a risk factor for ME/CFS.

Conclusion

This study reveals a causal relationship between genus paraprevotella, genus Ruminococcaceae_UCG_014 and ME/CFS, and our findings provide novel insights for further elucidating the developmental mechanisms mediated by the gut microbiota of ME/CFS.
Probiotics for the treatment of ulcerative colitis: a review of experimental research from 2018 to 2022
Authors: Huang Cuilan , Hao Wujuan , Wang Xuyang , Zhou Renmin , Lin Qiong
Published: July 2023

Ulcerative colitis (UC) has become a worldwide public health problem, and the prevalence of the disease among children has been increasing. The pathogenesis of UC has not been elucidated, but dysbiosis of the gut microbiota is considered the main cause of chronic intestinal inflammation. This review focuses on the therapeutic effects of probiotics on UC and the potential mechanisms involved. In animal studies, probiotics have been shown to alleviate symptoms of UC, including weight loss, diarrhea, blood in the stool, and a shortened colon length, while also restoring intestinal microecological homeostasis, improving gut barrier function, modulating the intestinal immune response, and attenuating intestinal inflammation, thereby providing theoretical support for the development of probiotic-based microbial products as an adjunctive therapy for UC. However, the efficacy of probiotics is influenced by factors such as the bacterial strain, dose, and form. Hence, the mechanisms of action need to be investigated further. Relevant clinical trials are currently lacking, so the extension of animal experimental findings to clinical application requires a longer period of consideration for validation.
Ubiquitin-mediated degradation at the Golgi apparatus
Authors: Buzuk Lana , Hellerschmied Doris
Published: July 2023

The Golgi apparatus is an essential organelle of the secretory pathway in eukaryotic cells. It processes secretory and transmembrane proteins and orchestrates their transport to other endomembrane compartments or the plasma membrane. The Golgi apparatus thereby shapes the cell surface, controlling cell polarity, cell-cell communication, and immune signaling. The cytosolic face of the Golgi hosts and regulates signaling cascades, impacting most notably the DNA damage response and mitosis. These essential functions strongly depend on Golgi protein homeostasis and Golgi integrity. Golgi fragmentation and consequent malfunction is associated with neurodegenerative diseases and certain cancer types. Recent studies provide first insight into the critical role of ubiquitin signaling in maintaining Golgi integrity and in Golgi protein quality control. Similar to well described pathways at the endoplasmic reticulum, ubiquitin-dependent degradation of non-native proteins prevents the accumulation of toxic protein aggregates at the Golgi. Moreover, ubiquitination regulates Golgi structural rearrangements in response to cellular stress. Advances in elucidating ubiquitination and degradation events at the Golgi are starting to paint a picture of the molecular machinery underlying Golgi (protein) homeostasis.
Safety and efficacy of brivaracetam in children epilepsy: a systematic review and meta-analysis
Authors: Song Ting , Feng Lingjun , Xia Yulei , Pang Meng , Geng Jianhong , Zhang Xiaojun , Wang Yanqiang
Published: July 2023

Background

Epilepsy is one of the most common neurological diseases, affecting people of any age. Although the treatments of epilepsy are more and more diverse, the uncertainty regarding efficacy and adverse events still exists, especially in the control of childhood epilepsy.

Methods

We performed a systematic review and meta- analysis following the Cochrane Handbook and preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. Four databases including PubMed, Embase, Web of Science and Cochrane library were searched. Studies reporting the use of brivaracetam monotherapy or adjuvant therapy in children (aged ≤18 years) were eligible for inclusion. Each stage of the review was conducted by two authors independently. Random-effects models were used to combine effect sizes for the estimation of efficacy and safety.

Results

A total of 1884 articles were retrieved, and finally 9 articles were included, enrolling 503 children with epilepsy. The retention rate of BRV treatment was 78% (95% CI: 0.64–0.91), the responder rate (reduction of seizure frequency ≥ 50%) was 35% (95% CI: 0.24–0.47), the freedom seizure rate (no seizure) was 18% (95% CI: 0.10–0.25), and the incidence rate of any treatment-emergent adverse events (TEAE) was 39% (95% CI: 0.09–0.68). The most common TEAE was somnolence, which had an incidence rate of 9% (95% CI: 0.07–0.12). And the incidence rate of mental or behavioral disorders was 12% (95% CI: 0.06–0.17).

Conclusion

Our systematic review and meta-analysis showed that BRV seemed to be safe and effective in the treatment of childhood epilepsy.
Primary pituitary stalk mucosa-associated lymphoid tissue lymphoma: a case report and literature review
Authors: Cai Shihao , Xiao Juexian , Chen Peng , Luo Haitao , Cheng Zujue
Published: July 2023

Background

Primary extranodal mucosa-associated lymphoid tissue (MALT) lymphoma in the sellar region is a rare indolent B-cell lymphoma.

Case presentation

A newly diagnosed patient with MALT lymphoma originating from the pituitary stalk is reported. A space-occupying lesion in the sellar region was found in a 24 year-old man who had no clinical symptoms except for those relating to a sex hormone disorder (rising estrogen and falling androgen) identified during a pre-employment physical examination. MALT lymphoma was diagnosed pathologically. Radiotherapy and chemotherapy were proposed after surgery. However, the patient selected androgen replacement therapy only rather than chemoradiotherapy. Over the next 3 months, no visual disturbance, headache, cranial nerve abnormality, or other symptoms occurred.

Conclusion

Primary sellar region MALT lymphoma is an extremely rare disease. The differential diagnosis of sellar and parasellar masses should include primary sellar region MALT lymphoma. Early detection and treatment of this lymphoma can effectively improve the prognosis.
Real-world experience with monthly and quarterly dosing of fremanezumab for the treatment of patients with migraine in Japan
Authors: Suzuki Shiho , Suzuki Keisuke , Shiina Tomohiko , Haruyama Yasuo , Hirata Koichi
Published: July 2023

Background

The effectiveness of fremanezumab in treating migraine has been demonstrated in randomized controlled trials. However, real-world study results are still limited.

Methods

We conducted a single-center, observational study that included patients with episodic migraine (EM) and chronic migraine (CM) who received fremanezumab monthly or quarterly over 6-month periods. The primary outcome of this study was to evaluate changes in monthly migraine days (MMD) and responder achievement after treatment with fremanezumab. The secondary aim was to characterize the predictors of responder at 6 months. We also evaluated the effectiveness of fremanezumab in the patients who switched from other calcitonin gene-related peptide (CGRP) monoclonal antibodies, and compared the effectiveness of fremanezumab between the monthly and quarterly dosing groups. One hundred twenty-seven patients with migraine (age, 45.2 ± 12.6 years; 96 women) who received at least one dose of fremanezumab with ≥3 months of follow-up were included. The number of MMD was assessed by headache diary.

Results

The changes in MMD from baseline at 1, 3, and 6 months were −6.1 ± 4.7, −7.7 ± 4.4, and − 8.5 ± 4.5 days in the total cohort, respectively (p < 0.001). The ≥50%, ≥ 75 and 100% responder rates at 6 months were 67.6, 22.5, and 5.4% in the total cohort, 90.4, 36.5, and 9.6% in the EM group, and 52.2, 14.9, and 1.5% in the CM group, respectively. Fremanezumab was also effective in 35 patients who switched from other CGRP monoclonal antibodies. Quarterly and monthly fremanezumab doses were equally effective in MMD reduction in the EM and CM groups. In the CM group, 65.1% experienced remission to EM after 6 months. Adverse reactions were mild and occurred in 9.5% of total patients. An at least ≥50% reduction in MMD from months 1 to 3 better predicted a ≥ 50% reduction in MMD at 6 months with 90.5% sensitivity and 80.6% specificity (p < 0.001).

Conclusion

In our real-world study, quarterly and monthly fremanezumab dosing showed both favorable effectiveness and tolerability in patients with migraine.
What over 1,000,000 participants tell us about online research protocols
Authors: Tomczak Johanna , Gordon Andrew , Adams Jamie , Pickering Jade S. , Hodges Nick , Evershed Jo K.
Published: July 2023

With the ever-increasing adoption of tools for online research, for the first time we have visibility on macro-level trends in research that were previously unattainable. However, until now this data has been siloed within company databases and unavailable to researchers. Between them, the online study creation and hosting tool Gorilla Experiment Builder and the recruitment platform Prolific hold metadata gleaned from millions of participants and over half a million studies. We analyzed a subset of this data (over 1 million participants and half a million studies) to reveal critical information about the current state of the online research landscape that researchers can use to inform their own study planning and execution. We analyzed this data to discover basic benchmarking statistics about online research that all researchers conducting their work online may be interested to know. In doing so, we identified insights related to: the typical study length, average completion rates within studies, the most frequent sample sizes, the most popular participant filters, and gross participant activity levels. We present this data in the hope that it can be used to inform research choices going forward and provide a snapshot of the current state of online research.
Short chain fatty acids: the messengers from down below
Authors: Mansuy-Aubert Virginie , Ravussin Yann
Published: July 2023

Short-chain fatty acids (SCFAs), produced by the metabolism of dietary fibers in the gut, have wide-ranging effects locally and throughout the body. They modulate the enteric and central nervous systems, benefit anti-inflammatory pathways, and serve as energy sources. Recent research reveals SCFAs as crucial communicators between the gut and brain, forming the gut-brain axis. This perspective highlights key findings and discusses signaling mechanisms connecting SCFAs to the brain. By shedding light on this link, the perspective aims to inspire innovative research in this rapidly developing field.
Recognizing emotions induced by wearable haptic vibration using noninvasive electroencephalogram
Authors: Wang Xin , Xu Baoguo , Zhang Wenbin , Wang Jiajin , Deng Leying , Ping Jingyu , Hu Cong , Li Huijun
Published: July 2023

The integration of haptic technology into affective computing has led to a new field known as affective haptics. Nonetheless, the mechanism underlying the interaction between haptics and emotions remains unclear. In this paper, we proposed a novel haptic pattern with adaptive vibration intensity and rhythm according to the volume, and applied it into the emotional experiment paradigm. To verify its superiority, the proposed haptic pattern was compared with an existing haptic pattern by combining them with conventional visual–auditory stimuli to induce emotions (joy, sadness, fear, and neutral), and the subjects’ EEG signals were collected simultaneously. The features of power spectral density (PSD), differential entropy (DE), differential asymmetry (DASM), and differential caudality (DCAU) were extracted, and the support vector machine (SVM) was utilized to recognize four target emotions. The results demonstrated that haptic stimuli enhanced the activity of the lateral temporal and prefrontal areas of the emotion-related brain regions. Moreover, the classification accuracy of the existing constant haptic pattern and the proposed adaptive haptic pattern increased by 7.71 and 8.60%, respectively. These findings indicate that flexible and varied haptic patterns can enhance immersion and fully stimulate target emotions, which are of great importance for wearable haptic interfaces and emotion communication through haptics.
Glymphatic system: an emerging therapeutic approach for neurological disorders
Authors: Gao Ying , Liu Kangding , Zhu Jie
Published: July 2023

The functions of the glymphatic system include clearance of the metabolic waste and modulation of the water transport in the brain, and it forms a brain-wide fluid network along with cerebrospinal fluid (CSF) and interstitial fluid (ISF). The glymphatic pathway consists of periarterial influx of CSF, astrocyte-mediated interchange between ISF and CSF supported by aquaporin-4 (AQP4) on the endfeet of astrocyte around the periarterioles, and perivenous efflux of CSF. Finally, CSF is absorbed by the arachnoid granules or flows into the cervical lymphatic vessels. There is growing evidence from animal experiments that the glymphatic system dysfunction is involved in many neurological disorders, such as Alzheimer’s disease, stroke, epilepsy, traumatic brain injury and meningitis. In this review, we summarize the latest progress on the glymphatic system and its driving factors, as well as changes in the glymphatic pathway in different neurological diseases. We significantly highlight the likely therapeutic approaches for glymphatic pathway in neurological diseases, and the importance of AQP4 and normal sleep architecture in this process.
Association between ultra-processed food intake and risk of colorectal cancer: a systematic review and meta-analysis
Authors: Shu Long , Huang Yiqian , Si Caijuan , Zhu Qin , Zheng Peifen , Zhang Xiaoyan
Published: July 2023

Background

Although some epidemiological studies have shown a positive relationship between high intake of ultra-processed food (UPF) and risk of colorectal cancer (CRC), the results remain inconsistent. Therefore, we conducted this systematic review and meta-analysis to clarify the association between UPF intake and CRC risk.

Methods

PubMed/MEDLINE, Web of Science, EMBASE, China National Knowledge Infrastructure (CNKI) and Wan fang databases were used to search the relevant studies published up to February 2023. The summary relative risks (RRs) with the corresponding 95% confidence intervals (CIs) were estimated by comparing the highest category vs. the lowest category of UPF intake, using the random-effects models (DerSimonian-Laird method). Heterogeneity between studies was explored using the Cochran’s Q test and I-square (I2). Publication bias was assessed by examining the funnel plots, and quantified by Begg’s or Egger’s tests.

Results

A total of seven articles (three cohort and four case-control studies), involving 18,673 CRC cases and 462,292 participants, were included in our study. Combining nine effect sizes from seven articles, an increased risk of CRC was shown in the highest compared with the lowest category of UPF intake (RR = 1.26; 95%CI:1.14–1.38, p < 0.0001). Subgroup analyses showed a positive association between UPF intake and CRC risk in case–control studies (RR = 1.41; 95%CI: 1.22–1.63, p < 0.0001). When we conducted analyses separately by study area, there was a significant association between UPF intake and CRC risk in developed countries (RR = 1.20; 95%CI: 1.11–1.30, p < 0.0001).

Conclusion

Our results show that high UPF intake is significantly associated with a higher risk of CRC, in the absence, however, of a dose–response association. Further studies in particular of large prospective cohort studies are necessary to confirm these results.
Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the faecal metabolome in children with coeliac disease autoimmunity: a randomised, double-blinded placebo-controlled clinical trial
Authors: Jenickova Eliska , Andrén Aronsson Carin , Mascellani Bergo Anna , Cinek Ondrej , Havlik Jaroslav , Agardh Daniel
Published: July 2023

Introduction

Coeliac disease is a lifelong immune-mediated enteropathy manifested as gluten intolerance in individuals carrying specific human leukocyte antigen (HLA) molecules. Other factors than genetics and gluten intake, however, may play a role in triggering the disease. The gut internal environment is thought to be one of these potential contributing factors, and it can be influenced throughout life.

Methods

We examine the impact of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 supplementation on the faecal metabolome in genetically predisposed children having tissue transglutaminase autoantibodies, i.e., coeliac disease autoimmunity. Probiotic strains were selected based on their beneficial properties, including mucosal permeability and immune modulation effects. The intervention group (n = 40) and control group (n = 38) took the probiotics or placebo daily for 6 months in a double-blinded randomised trial. Faecal samples were collected at baseline and after 3 and 6 months and analysed using the 1H NMR for metabolome. The incorporation of 16S rRNA sequencing as a supportive dataset complemented the analysis of the metabolome data.

Results

During the 6 months of intervention, the stool concentrations of 4-hydroxyphenylacetate increased in the intervention group as compared to controls, whereas concentrations of threonine, valine, leucine, isoleucine, methionine, phenylalanine, aspartate, and fumarate decreased. Additionally, a noteworthy effect on the glycine, serine, and threonine metabolic pathway has been observed.

Conclusion

The findings suggest a modest yet significant impact of the probiotics on the faecal metabolome, primarily influencing proteolytic processes in the gut.

Clinical trial registration

ClinicalTrials.gov, NCT03176095.
Rare variants confer shared susceptibility to gastrointestinal tract cancer risk
Authors: Zheng Ji , Wang Xin , Li Jingrao , Wu Yuanna , Chang Jiang , Xin Junyi , Wang Meilin , Wang Tianpei , Wei Qingyi , Wang Mengyun , Zhang Ruoxin
Published: July 2023

Background

Cancers arising within the gastrointestinal tract are complex disorders involving genetic events that cause the conversion of normal tissue to premalignant lesions and malignancy. Shared genetic features are reported in epithelial-based gastrointestinal cancers which indicate common susceptibility among this group of malignancies. In addition, the contribution of rare variants may constitute parts of genetic susceptibility.

Methods

A cross-cancer analysis of 38,171 shared rare genetic variants from genome-wide association assays was conducted, which included data from 3,194 cases and 1,455 controls across three cancer sites (esophageal, gastric and colorectal). The SNP-level association was performed by multivariate logistic regression analyses for single cancer, followed by association analysis for SubSETs (ASSET) to adjust the bias of overlapping controls. Gene-level analyses were conducted by SKAT-O, with multiple comparison adjustments by false discovery rate (FDR). Based on the significant genes indicated by SKATO analysis, pathways analysis was conducted using Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Reactome databases.

Results

Meta-analysis in three gastrointestinal (GI) cancers identified 13 novel susceptibility loci that reached genome-wide significance (PASSET< 5×10-8). SKAT-O analysis revealed EXOC6, LRP5L and MIR1263/LINC01324 to be significant genes shared by GI cancers (Padj<0.05, PFDR<0.05). Furthermore, GO pathway analysis identified significant enrichment of synaptic transmission and neuron development pathways shared by all three cancer types.

Conclusion

Rare variants and the corresponding genes potentially contribute to shared susceptibility in different GI cancer types. The discovery of these novel variants and genes offers new insights for the carcinogenic mechanisms and missing heritability of GI cancers.
Early onset, development and histological features of gastric signet-ring cell carcinoma
Authors: Wang Yangkun , Li Yingying , Wang Bin , Ran Dongmei , Zhu Chaoya , Li Ping , Jiang Bo , Wang Sunan
Published: July 2023

Objective

To explore the early onset, development and histological features of gastric signet-ring cell carcinoma (SRCC).

Methods

Three hundred and sixty-two patients with differentiated adenocarcinoma with signet-ring cells were enrolled. Histomorphological and immunohistochemical features and patterns of the specimens were observed in detail.

Results

Infection of the gastric mucosa, especially by Helicobacter pylori, can cause massive cell proliferation and transformation in the deep gastric foveola, the isthmus of the gastric gland, and the proliferative zone of the upper neck of the gland. Signet-ring-like heterocysts monoclonally proliferated after the redifferentiation and reproliferation, extending horizontally along the gastric foveola. Gastric foveolar-type SRCC grew infiltratively into the lamina propria of the mucosa and the submucosa, signet-ring cells could differentiate into undifferentiated adenocarcinoma with signet-ring cell differentiation, mucinous adenocarcinoma with signet-ring cell differentiation, gastric adenocarcinoma with signet-ring cell differentiation, and fundus gland adenocarcinoma with signet-ring cell differentiation.

Conclusion

Early SRCC developed from the proliferative zones of the fundus of the gastric foveola and the neck of the gastric gland, growing horizontally along the gastric foveola. It developed into gastric adenocarcinoma with signet-ring cell differentiation after reproliferation and retransformation in the mucosa.
Insight into lipid-based nanoplatform-mediated drug and gene delivery in neuro-oncology and their clinical prospects
Authors: Hegde Manasa Manjunath , Sandbhor Puja , J. Aishwarya , Gota Vikram , Goda Jayant S.
Published: July 2023

Tumors of the Central nervous System (CNS) are a spectrum of neoplasms that range from benign lesions to highly malignant and aggressive lesions. Despite aggressive multimodal treatment approaches, the morbidity and mortality are high with dismal survival outcomes in these malignant tumors. Moreover, the non-specificity of conventional treatments substantiates the rationale for precise therapeutic strategies that selectively target infiltrating tumor cells within the brain, and minimize systemic and collateral damage. With the recent advancement of nanoplatforms for biomaterials applications, lipid-based nanoparticulate systems present an attractive and breakthrough impact on CNS tumor management. Lipid nanoparticles centered immunotherapeutic agents treating malignant CNS tumors could convene the clear need for precise treatment strategies. Immunotherapeutic agents can selectively induce specific immune responses by active or innate immune responses at the local site within the brain. In this review, we discuss the therapeutic applications of lipid-based nanoplatforms for CNS tumors with an emphasis on revolutionary approaches in brain targeting, imaging, and drug and gene delivery with immunotherapy. Lipid-based nanoparticle platforms represent one of the most promising colloidal carriers for chemotherapeutic, and immunotherapeutic drugs. Their current application in oncology especially in brain tumors has brought about a paradigm shift in cancer treatment by improving the antitumor activity of several agents that could be used to selectively target brain tumors. Subsequently, the lab-to-clinic transformation and challenges towards translational feasibility of lipid-based nanoplatforms for drug and gene/immunotherapy delivery in the context of CNS tumor management is addressed.
How does the pattern of root metabolites regulating beneficial microorganisms change with different grazing pressures?
Authors: Yuan Ting , Ren Weibo , Wang Zhaoming , Fry Ellen L. , Tang Shiming , Yin Jingjing , Zhang Jiatao , Jia Zhenyu
Published: July 2023

Grazing disturbance can change the structure of plant rhizosphere microbial communities and thereby alter the feedback to promote plant growth or induce plant defenses. However, little is known about how such changes occur and vary under different grazing pressures or the roles of root metabolites in altering the composition of rhizosphere microbial communities. In this study, the effects of different grazing pressures on the composition of microbial communities were investigated, and the mechanisms by which different grazing pressures changed rhizosphere microbiomes were explored with metabolomics. Grazing changed composition, functions, and co-expression networks of microbial communities. Under light grazing (LG), some saprophytic fungi, such as Lentinus sp., Ramichloridium sp., Ascobolus sp. and Hyphoderma sp., were significantly enriched, whereas under heavy grazing (HG), potentially beneficial rhizobacteria, such as Stenotrophomonas sp., Microbacterium sp., and Lysobacter sp., were significantly enriched. The beneficial mycorrhizal fungus Schizothecium sp. was significantly enriched in both LG and HG. Moreover, all enriched beneficial microorganisms were positively correlated with root metabolites, including amino acids (AAs), short-chain organic acids (SCOAs), and alkaloids. This suggests that these significantly enriched rhizosphere microbial changes may be caused by these differential root metabolites. Under LG, it is inferred that root metabolites, especially AAs such as L-Histidine, may regulate specific saprophytic fungi to participate in material transformations and the energy cycle and promote plant growth. Furthermore, to help alleviate the stress of HG and improve plant defenses, it is inferred that the root system actively regulates the synthesis of these root metabolites such as AAs, SCOAs, and alkaloids under grazing interference, and then secretes them to promote the growth of some specific plant growth-promoting rhizobacteria and fungi. To summarize, grasses can regulate beneficial microorganisms by changing root metabolites composition, and the response strategies vary under different grazing pressure in typical grassland ecosystems.
OsHIPP17 is involved in regulating the tolerance of rice to copper stress
Authors: Shi Yang , Jiang Nan , Wang Mengting , Du Zhiye , Chen Ji , Huang Yanyan , Li Mingyu , Jin Yufan , Li Jiahao , Wan Jian , Jin Xiaowan , Zhang Lang , Huang Jin
Published: July 2023

Introduction

Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in metal absorption, transport and accumulation in plants. However, so far, only several plant HIPPs have been functionally analyzed. In this study, a novel HIPP member OsHIPP17, which was involved in the tolerance to copper (Cu) was functionally characterized.

Methods

In this study, qRT-PCR, Yeast transgenic technology, Plant transgenic technology, ICP-MS and so on were used for research.

Results

OsHIPP17 protein was targeted to the nucleus. The Cu concentration reached 0.45 mg/g dry weight due to the overexpression of OsHIPP17 in yeast cells. Meanwhile, the overexpression of OsHIPP17 resulted in the compromised growth of Arabidopsis thaliana (Arabidopsis) under Cu stress. The root length of Oshipp17 mutant lines was also significantly reduced by 16.74- 24.36% under 25 mM Cu stress. The roots of Oshipp17 rice mutant showed increased Cu concentration by 7.25%-23.32%. Meanwhile, knockout of OsHIPP17 decreased the expression levels of OsATX1, OsZIP1, OsCOPT5 or OsHMA5, and increased the expression levels of OsCOPT1 or OsHMA4. Antioxidant enzyme activity was also reduced in rice due to the knockout of OsHIPP17. Moreover, the expression levels of cytokinin-related genes in plants under Cu stress were also affected by overexpression or knockout of OsHIPP17.

Discussion

These results implied that OsHIPP17 might play a role in plant Cu toxic response by affecting the expression of Cu transport genes or cytokinin-related genes. Simultaneously, our work may shed light on the underlying mechanism of how heavy metals affect the plant growth and provide a novel rice genetic source for phytoremediation of heavy metal-contaminated soil.
Characterization of a barley (Hordeum vulgare L.) mutant with multiple stem nodes and spikes and dwarf (msnsd) and fine-mapping of its causal gene
Authors: Qin Dandan D. , Liu Rui , Xu Fuchao , Dong Guoqing , Xu Qing , Peng Yanchun , Xu Le , Cheng Hongna , Guo Ganggang , Dong Jing , Li Chengdao
Published: July 2023

Introduction

Multiple nodes and dwarf mutants in barley are a valuable resource for identifying genes that control shoot branching, vegetative growth and development.

Methods

In this study, physiological, microscopic and genetic analysis were conducted to characterize and fine-map the underling gene of a barley mutant with Multiple Stem Nodes and Spikes and Dwarf (msnsd), which was selected from EMS- and 60Co-treated barley cv. Edamai 934.

Results and discussion

The msnsd mutant had more stem nodes, lower plant height and a shorter plastochron than Edamai 934. Moreover, the mutant had two or more spikes on each tiller. Microscopic analysis showed that the dwarf phenotype of msnsd resulted from reduced cell lengths and cell numbers in the stem. Further physiological analysis showed that msnsd was GA3-deficient, with its plant height increasing after external GA3 application. Genetic analysis revealed that a single recessive nuclear gene, namely, HvMSNSD, controlled the msnsd phenotype. Using a segregating population derived from Harrington and the msnsd mutant, HvMSNSD was fine-mapped on chromosome 5H in a 200 kb interval using bulked segregant analysis (BSA) coupled with RNA-sequencing (BSR-seq), with a C-T substitution in the exon of HvTCP25 co-segregating with the msnsd phenotype. RNA-seq analysis showed that a gene encoding gibberellin 2-oxidase 8, a negative regulator of GA biosynthesis, was upregulated in the msnsd mutant. Several known genes related to inflorescence development that were also upregulated and enriched in the msnsd mutant. Collectively, we propose that HvMSNSD regulates the plastochron and morphology of reproductive organs, likely by coordinating GA homeostasis and changed expression of floral development related genes in barley. This study offers valuable insights into the molecular regulation of barley plant architecture and inflorescence development.
The role of recombination landscape in species hybridisation and speciation
Authors: Wong Edgar L. Y. , Filatov Dmitry A.
Published: July 2023

It is now well recognised that closely related species can hybridize and exchange genetic material, which may promote or oppose adaptation and speciation. In some cases, interspecific hybridisation is very common, making it surprising that species identity is preserved despite active gene exchange. The genomes of most eukaryotic species are highly heterogeneous with regard to gene density, abundance of repetitive DNA, chromatin compactisation etc, which can make certain genomic regions more prone or more resistant to introgression of genetic material from other species. Heterogeneity in local recombination rate underpins many of the observed patterns across the genome (e.g. actively recombining regions are typically gene rich and depleted for repetitive DNA) and it can strongly affect the permeability of genomic regions to interspecific introgression. The larger the region lacking recombination, the higher the chance for the presence of species incompatibility gene(s) in that region, making the entire non- or rarely recombining block impermeable to interspecific introgression. Large plant genomes tend to have highly heterogeneous recombination landscape, with recombination frequently occurring at the ends of the chromosomes and central regions lacking recombination. In this paper we review the relationship between recombination and introgression in plants and argue that large rarely recombining regions likely play a major role in preserving species identity in actively hybridising plant species.
School climate and adolescents’ prosocial behavior: the mediating role of perceived social support and resilience
Authors: Luo Yun , Ma Tangsheng , Deng Yuting
Published: July 2023

Prosocial behavior is crucial for adolescent socialization and plays a positive role in all aspects of adolescent development. Based on ecosystem theory and self-determination theory, this study aimed to explore the relationship among school climate, perceived social support, psychological resilience, and prosocial behavior. With 1,688 high school students being sampled, we utilized the Perceived School Climate Questionnaire, the Perceived Social Support Scale, the Chinese Version of Mental Resilience Scale, and the Adolescent Prosocial Tendency Scale. The results showed that: (1) school climate, perceived social support, psychological resilience, and prosocial behavior were all positively correlated; (2) perceived social support and psychological resilience could independently mediate the relationship between school climate and prosocial behavior; these two mediating variables could develop a chain mediation effect to influence the link between school climate and prosocial behavior.
Cognitive rationalization in occupational fraud: structure exploration and scale development
Authors: Yang Miao , Chen Yizao
Published: July 2023

The structure and measurement of occupational fraud rationalization as one of the motivations for fraudulent behavior has been a major obstacle in theoretical research and practical problems. In order to answer the fundamental question, “What does cognitive rationalization of occupational fraud involve?,” this paper explored the structure and scale development of the internal psychological factors of occupational fraud rationalization. Several research methods were used for this purpose, such as data collection, research interviews, review & verification, project purification, structural verification, and reliability & validity test. The results showed that, based on the internal structure, occupational fraud rationalization presented second-order three-dimensional and first-order eight-dimensional factors. Further, a formal scale containing 27 items related to the structure of the metric was constructed to measure the occupational fraud rationalization. In terms of variable correlations, this paper empirically tested the criterion validity of occupational fraud rationalization from the perspective of personality traits. The result revealed a significant positive (negative) correlation between Machiavellian traits (empathy traits) and occupational fraud rationalization, respectively. In conclusion, this paper provides an attempt to address the cognitive and measurement challenges of occupational fraud rationalization, expanding the application and development of moral disengagement theory in the field of occupational fraud and laying some groundwork for subsequent research and development.
Can work-to-family conflict lead to preschool children’s social behavior problems?—The chain mediating roles of guilt about parenting and parent-child relationships
Authors: Wang Yan , Shi Dasheng , Liu Guolei , Zhang Mengmeng , Zheng Xinhong
Published: July 2023

Parents’ work-to-family conflict has been reported to be associated with preschool children’s social behavior problems, but the underlying mechanisms of this association in the Chinese cultural context remain unclear. Based on ecosystem theory and the spillover-crossover model theory of emotion, this study aimed to examine the correlation between parents’ work-to-family conflict and preschool children’s social behavior problems in China, as well as the mediating role of guilt about parenting and parent–child relationships. Structural equation modeling was used to check the research hypotheses with a sample of 3,038 parents of Chinese preschool children. The main findings of this study are as follows: (1) Work-to-family conflict faced by parents was positively associated with guilt about parenting and preschool children’s social behavior problems; (2) The effect of guilt about parenting on preschool children’s social behavior problems was bidirectional; guilt about parenting was positively related to preschool children’s social behavior problems, but when guilt about parenting prompted parents to adjust their parent–child relationships, it was negatively related to preschool children’s social behavior problems. Taken together, these results further explain the interaction between parents’ work-to-family conflict and preschool children’s social behavior problems and discuss the influence of multiple factors on preschool children’s social behavior problems. Theoretically, this study enriches the theoretical basis of the interaction with resources from the external environment of home education and family education. Practically, it implies that multiple levels, such as the government, early childhood education institutions, and work units, should give more support to preschool children’s family education and thus work together to promote the healthy development of preschool children.
The Methodological Quality Scale (MQS) for intervention programs: validity evidence
Authors: Chacón-Moscoso Salvador , Sanduvete-Chaves Susana , Lozano-Lozano José Antonio , Holgado-Tello Francisco Pablo
Published: July 2023

Introduction

A wide variety of instruments are used when assessing the methodological quality (MQ) of intervention programs. Nevertheless, studies on their metric quality are often not available. In order to address this shortcoming, the methodological quality scale (MQS) is presented as a simple and useful tool with adequate reliability, validity evidence, and metric properties.

Methods

Two coders independently applied the MQS to a set of primary studies. The number of MQ facets was determined in parallel analyses before performing factor analyses. For each facet of validity obtained, mean and standard deviation are presented jointly with reliability and average discrimination. Additionally, the validity facet scores are interpreted based on Shadish, Cook, and Campbell’s validity model.

Results and discussion

An empirical validation of the three facets of the MQ (external, internal, and construct validity) and the interpretation of the scores were obtained based on a theoretical framework. Unlike other existing scales, MQS is easy to apply and presents adequate metric properties. In addition, MQ profiles can be obtained in different areas of intervention using different methodologies and proves useful for both researchers doing meta-analysis and for evaluators and professionals designing a new intervention.
Solitude profiles and psychological adjustment in Chinese late adolescence: a person-centered research
Authors: Zhou Tong , Liao Longyue , Nguyen Thuy-Vy T. , Li Dan , Liu Junsheng
Published: July 2023

Objectives

From the perspective of person-centered research, the present study aimed to identify the potential profiles of solitude among late adolescents based on their solitary behavior, motivation, attitude, and time alone. In addition, to echo the paradox of solitude, we further explored the links between solitude profiles and adjustment outcomes.

Methods

The participants of the study were 355 late adolescents (56.34% female, Mage = 19.71 years old) at three universities in Shanghai, China. Measures of solitary behavior, autonomous motivation for solitude, attitude toward being alone, and time spent alone were collected using adolescents' self-report assessments. The UCLA Loneliness Scale, the Beck Depression Inventory, and the Basic Psychological Needs Scales were measured as indices of adjustment.

Results

Latent profile analysis revealed four distinct groups: absence of the aloneness group (21.13%), the positive motivational solitude group (29.01%), the negative motivational solitude group (38.03%), and the activity-oriented solitude group (11.83%). Differences emerged among these four groups in terms of loneliness, depressive symptoms, and basic needs satisfaction, with adolescents in the negative motivational solitude group facing the most risk of psychological maladjustment.

Conclusion

Findings revealed the possible heterogeneous nature of solitude among Chinese late adolescents and provided a theoretical basis for further understanding of adolescents' solitary state.
Pathogenetic characteristics of infectious diarrhea in Yantai City, Shandong Province, 2018–2019
Authors: Gao Qiao , Liu Hong , Yu Weixia , Wang Zhaolin , Yang Ying , Guo Kai , Sun Zhenlu
Published: July 2023

Background

Analysis of the pathogenic spectrum, epidemiological characteristics and molecular epidemiological features of important pathogens of infectious diarrhea in Yantai City, Shandong Province, 2018–2019, were analyzed to provide a reference basis for the prevention and control of infectious diarrhea.

Methods

A total of 1514 stool specimens were collected within 3 days of the onset of diarrhea in secondary or higher hospitals in Yantai from 2018–2019, and all specimens were tested for the presence of seven major viruses and three major bacteria by employing reverse transcription ploymeraer chain reaction (RT-PCR). Population and seasonal analyses were also performed on positive samples for infectious diarrhea. The top two ranked rotavirus and norovirus were focused on genotyping and analysis of geographical distribution.

Results

The study showed that the high prevalence of infectious diarrhea in Yantai, Shandong Province, for two consecutive years in 2018 and 2019 was in young children aged 1–5 years, accounting for 48.6% of the total number of cases. Viral diarrhea was distributed throughout the year with no obvious seasonal distribution, while bacterial diarrhea was predominant in summer. Of 1514 stool specimens, the total positive rate of specimens was 43.92% (665/1514). One pathogen was detected in 507 specimens, two pathogens in 107 specimens, and three pathogens in 44 specimens, with mixed infections accounting for 22.71% of positive specimens (151/665). Viral diarrheal pathogens accounted for 93.23% (620/665) of positive samples. The percentages of positive samples for Rotavirus (RV), Norovirus GI, Norovirus GII, Enterovirus universal (EV), Enteroadenovirus (EAdV), Sapovirus (SaV), Astrovirus (Astv), Salmonella (SE), Listeria monocytogenes (LiMo), and Vibrio parahaemolyticus (VP) were 48.57%, 3.61%, 15.34%, and 10.68% of the total positive samples.

Conclusions

This study analyzed in detail the composition of infectious diarrhea pathogen spectrum, pathogen alternation pattern, seasonal distribution and population distribution of pathogens in Yantai City, Shandong Province, 2018–2019, to provide a basis for improving relevant local preventive measures and reducing the disease burden.
State of vulnerable populations in the techquity framework in Hungary
Authors: Győrffy Zsuzsa , Döbrössy Bence , Radó Nóra , Boros Julianna , Békási Sándor
Published: July 2023

Digital health solutions could alleviate the needs of vulnerable populations in the recent period of the permacrisis, however, there are several barriers that limit their use for certain individuals. We use the four-pillar model of the novel concept of techquity to provide original evidence of the discrepancy in the willingness to try and the ability to harness healthtech in Hungary. We identified three underserved segments of society: older adults, people with long-term activity-limiting conditions, and people experiencing homelessness who could greatly benefit from digital technologies and yet use them less than the general population. We also discuss potential strategic considerations in order to promote techquity and digital inclusion among people living in vulnerable situations.
Analyzing scenarios and designing initiatives toward just transitions: coproducing knowledge with(in) the dried fish sector in the Indian Sundarbans
Authors: Ghosh Raktima , Mukherjee Jenia , Bandyopadhyay Aishik , Chatterjee Subhradeep , Choudry Anuradha , Ghosh Poulami , Pathak Souradip , Sen Amrita , Sinha Priyadarsini
Published: July 2023

The south-western fringe of the Indian Sundarbans hosts a number of fish drying collectives that are exposed to a bundle of mutually reinforcing social-ecological-institutional risks. Despite its wide contribution to local-regional food security and livelihoods, dried fish production has received little attention in research and policy circles so far. As part of the Social Sciences and Humanities Research Council (SSHRC) sponsored Dried Fish Matters global partnership project, our research team seeks to address this lack in two ways: (i) delineating knowledge base through a systematic literature review on intersecting social-ecological and sociohydrological dimensions of integrated fisheries and fish drying practices, and (ii) employing a knowledge co-production approach that involves participation of dry-fishers, researchers, fishworkers' forum (partner organization), and scientists for meaningful understandings about the constraints and potentials in the sector. In this essay, we applied a three-step methodology to arrive at a crisscrossing conceptual, empirical, and collective understandings on the 'invisible' dried fish value chain in the Indian Sundarbans. With detailed lessons from the field, our interdisciplinary research team acts as a liaison among the groups to build a collaborative space for interactions, recognize prevalent adaptive practices and identify pathways toward short-, intermediate-, and long-term co-interventions through which fish drying practices can be more effectively improvised upon and up scaled. The essay lays out detailed insights and sensible recommendations from the knowledge co-production workshop, organized as a part of solution-focused participatory research on climate-resilient and gender-aware dried fish practices in the Indian Sundarbans. In light of the collective observations on the complex problems and reflections on needs-driven initiatives, the authors advocate for collaborative research praxes in forging just transitions for the less explored dried fish sector.