bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel RFID Authentication Protocol Based on Reconfigurable RRAM PUF
Authors: Ren Qirui , Fu Xiangqu , Wu Hao , Yang Kaiqi , Lei Dengyun , Xing Guozhong , Zhang Feng
Published: December 2021

Radio frequency identification technology (RFID) has empowered a wide variety of automation industries. Aiming at the current light-weight RFID encryption scheme with limited information protection methods, combined with the physical unclonable function (PUF) composed of resistive random access memory (RRAM), a new type of high-efficiency reconfigurable strong PUF circuit structure is proposed in this paper. Experimental results show that the proposed PUF shows an almost ideal value (50%) of inter-chip hamming distance (HD) (µ/σ = 0.5001/0.0340) among 1000 PUF keys, and intra-chip HD results are very close to the ideal value (0). The bit error rate (BER) is as low as 3.8×10−6 across one million challenges. Based on the RRAM PUF, we propose and implement a light weight RFID authentication protocol. By virtue of RRAM’s model ability, the protocol replaces the One-way Hash Function with a response chain mutual encryption algorithm. The results of test and analysis show that the protocol can effectively resist multiple threats such as physical attacks, replay attacks, tracking attacks and asynchronous attacks, and has good stability. At the same time, based on RRAM’s unique resistance variability, PUF also has the advantage of being reconfigurable, providing good security for RFID tags.
Controls on Barite Mineralization in a Major Intracontinental Shear Zone: Carboniferous of the Cobequid Highlands, Nova Scotia
Authors: Pe-Piper Georgia , Piper David
Published: December 2021

Prominent veins of late Carboniferous barite, associated with fluorite and calcite, outcrop close to older granite plutons along an intracontinental shear zone that was active throughout the Carboniferous in southeastern Canada. Some barite is stratigraphically constrained to younger than 315 Ma and final mineralization is constrained by a published Rb–Sr isochron of 300 ± 6 Ma. Barite occurrences in the Carboniferous basins of central Nova Scotia, 50 km to the south, are synchronous with or post-date ankerite-siderite-magnetite-pyrolusite and Pb-Zn mineralization, which was facilitated by fluid interaction with thick evaporites. This study aims to determine controls on the distribution of barite in the shear zone, from field relationships, vein petrography and isotope geochemistry of minerals. The isotope chemistry of shear zone barite is similar to that occurring in Pb-Zn-Mn-Ba mineralization to the south, suggesting a common origin. Veins of barite, associated with fluorite, represent the youngest and regionally coolest phase of a 70 Ma history of Carboniferous mineralized veins along the Minas Fault Zone. Their prominence close to granite plutons reflects brittle deformation of the deeply-rooted granites in a complexly deforming fault zone, but the origin of abundant F remains uncertain.
Product Development of a Rock Reinforcing Bolt for Underground Hard Rock Mining
Authors: Tshitema Ndalamo , Kallon Daramy Vandi Von
Published: December 2021

The demand for mineral resources has dramatically increased over the past few decades; this increase directly correlates to an increase in underground mining activity. There are different mining methods for different minerals, and each have their risks. In hard rock mining activities such as mining for gold, rockfalls are the most significant deterrent to obtaining mineral resources. This paper focuses on rock reinforcement systems to prevent fatal rockfalls in underground excavations. Presently, there is a global steel shortage and an increase in prices that has impacted the productivity of the mining operations that support most national economies. The paper’s main objective is to present the improvement of a rock bolt design used to support the roof in underground mining activities and keep working personnel and equipment safe from rockfalls. This study presents two rock bolt designs: a preliminary design and an improved model of the rock bolt. The paper discusses the operation of the rock bolt and provides laboratory test results on the bolt in operation. The principle of operation of the yield bolt is based on the science of radial expansion of hollow tubes in tension, to provide integrity to underground excavations. This functional design of the rock bolt requires less steel and has the same performance as the current rock reinforcement elongates. The research methodology involved interviewing rock mining engineers to determine their desired rock reinforcement device that would adequately meet the unpredictable dynamic and static behavior of underground rocks. The methodology also included experimental tests of a rock bolt design that was aimed at meeting the desired and acceptable performance determined from the interviews. The experimental results were obtained from a 60-ton hydraulic press that simulated seismic activity underground. The experimental results showed several modes of failure for the bolt; however, the improved rock bolt yielded at an average of 200 kN, as designed. During testing of the preliminary bolt design, there were failures that resulted from the manufacturing process of the bolt, such as splitting of the tube due to the welded end components. After a dynamic test, the preliminary bolt tube bent, creating huge forces on the tube which may cause fracture. The coefficient of friction during dynamic testing was lower than during static testing, leading to undesirable results for the preliminary bolt. The optimized bolt design addressed the failures and the low yield tonnage of the preliminary bolt design. It successfully yielded at 20 tons, even during the dynamic event. The bolt had similar alignment issues which caused failure during testing, as can be seen from the results. A guide tube was implemented in the design and the manufacturing process changed; these changes resulted in the bolt having a more reliable performance that met the requirements throughout.
Assembling Ultrafine SnO2 Nanoparticles on MIL-101(Cr) Octahedrons for Efficient Fuel Photocatalytic Denitrification
Authors: Liang Ruowen , Wang Shihui , Lu Yi , Yan Guiyang , He Zhoujun , Xia Yuzhou , Liang Zhiyu , Wu Ling
Published: December 2021

Effectively reducing the concentration of nitrogen-containing compounds (NCCs) remains a significant but challenging task in environmental restoration. In this work, a novel step-scheme (S-scheme) SnO2@MCr heterojunction was successfully fabricated via a facile hydrothermal method. At this heterojunction, MIL-101(Cr) octahedrons are decorated with highly dispersed SnO2 quantum dots (QDs, approximate size 3 nm). The QDs are evenly wrapped around the MIL-101(Cr), forming an intriguing zero-dimensional/three-dimensional (0D/3D) S-scheme heterostructure. Under simulated sunlight irradiation (280 nm < λ < 980 nm), SnO2@MCr demonstrated superior photoactivity toward the denitrification of pyridine, a typical NCC. The adsorption capacity and adsorption site of SnO2@MCr were also investigated. Tests using 20%SnO2@MCr exhibited much higher activity than that of pure SnO2 and MIL-101(Cr); the reduction ratio of Cr(VI) is rapidly increased to 95% after sunlight irradiation for 4 h. The improvement in the photocatalytic activity is attributed to (i) the high dispersion of SnO2 QDs, (ii) the binding of the rich adsorption sites with pyridine molecules, and (iii) the formation of the S-scheme heterojunction between SnO2 and MIL-101(Cr). Finally, the photocatalytic mechanism of pyridine was elucidated, and the possible intermediate products and degradation pathways were discussed.
Alkali-Activated Hybrid Cements Based on Fly Ash and Construction and Demolition Wastes Using Sodium Sulfate and Sodium Carbonate
Authors: Valencia-Saavedra William , Robayo-Salazar Rafael , Mejía de Gutiérrez Ruby
Published: December 2021

This article demonstrates the possibility of producing alkali-activated hybrid cements based on fly ash (FA), and construction and demolition wastes (concrete waste, COW; ceramic waste, CEW; and masonry waste, MAW) using sodium sulfate (Na2SO4) (2–6%) and sodium carbonate (Na2CO3) (5–10%) as activators. From a mixture of COW, CEW, and MAW in equal proportions (33.33%), a new precursor called CDW was generated. The precursors were mixed with ordinary Portland cement (OPC) (10–30%). Curing of the materials was performed at room temperature (25 °C). The hybrid cements activated with Na2SO4 reached compressive strengths of up to 31 MPa at 28 days of curing, and the hybrid cements activated with Na2CO3 yielded compressive strengths of up to 22 MPa. Based on their mechanical performance, the optimal mixtures were selected: FA/30OPC-4%Na2SO4, CDW/30OPC-4%Na2SO4, FA/30OPC-10%Na2CO3, and CDW/30OPC-10%Na2CO3. At prolonged ages (180 days), these mixtures reached compressive strength values similar to those reported for pastes based on 100% OPC. A notable advantage is the reduction of the heat of the reaction, which can be reduced by up to 10 times relative to that reported for the hydration of Portland cement. These results show the feasibility of manufacturing alkaline-activated hybrid cements using alternative activators with a lower environmental impact.
Suppressing Alpha-Hemolysin as Potential Target to Screen of Flavonoids to Combat Bacterial Coinfection
Authors: He Shangwen , Deng Qian , Liang Bingbing , Yu Feike , Yu Xiaohan , Guo Dawei , Liu Xiaoye , Dong Hong
Published: December 2021

The rapid emergence of bacterial coinfection caused by cytosolic bacteria has become a huge threat to public health worldwide. Past efforts have been devoted to discover the broad-spectrum antibiotics, while the emergence of antibiotic resistance encourages the development of antibacterial agents. In essence, bacterial virulence is a factor in antibiotic tolerance. However, the discovery and development of new antibacterial drugs and special antitoxin drugs is much more difficult in the antibiotic resistance era. Herein, we hypothesize that antitoxin hemolytic activity can serve as a screening principle to select antibacterial drugs to combat coinfection from natural products. Being the most abundant natural drug of plant origins, flavonoids were selected to assess the ability of antibacterial coinfections in this paper. Firstly, we note that four flavonoids, namely, baicalin, catechin, kaempferol, and quercetin, have previously exhibited antibacterial abilities. Then, we found that baicalin, kaempferol, and quercetin have better inhibitions of hemolytic activity of Hla than catechin. In addition, kaempferol and quercetin, have therapeutic effectivity for the coinfections of Staphylococcus aureus and Pseudomonas aeruginosa in vitro and in vivo. Finally, our results indicated that kaempferol and quercetin therapied the bacterial coinfection by inhibiting S. aureus α-hemolysin (Hla) and reduced the host inflammatory response. These results suggest that antitoxins may play a promising role as a potential target for screening flavonoids to combat bacterial coinfection.
High-Throughput Analysis of Amino Acids for Protein Quantification in Plant and Animal-Derived Samples Using High Resolution Mass Spectrometry
Authors: Reddy Priyanka , Elkins Aaron , Panozzo Joe , Rochfort Simone J.
Published: December 2021

Current methods for measuring the abundance of proteogenic amino acids in plants require derivatisation, extended run times, very sensitive pH adjustments of the protein hydrolysates, and the use of buffers in the chromatographic phases. Here, we describe a fast liquid chromatography–mass spectrometry (LC–MS) method for the determination of amino acids that requires only three steps: hydrolysis, neutralisation, and sample dilution with a borate buffer solution for pH and retention time stability. The method shows excellent repeatability (repeated consecutive injections) and reproducibility (repeated hydrolysis) in the amino acid content, peak area, and retention time for all the standard amino acids. The chromatographic run time is 20 min with a reproducibility and repeatability of <1% for the retention time and <11% for the peak area of the BSA and quality control (QC) lentil samples. The reproducibility of the total protein levels in the hydrolysis batches 1–4 was <12% for the BSA and the lentil samples. The level of detection on column was below 0.1 µM for most amino acids (mean 0.017 µM).
Effect of Juglone against Pseudomonas syringae pv Actinidiae Planktonic Growth and Biofilm Formation
Authors: Han Qiqi , Feng Luoluo , Zhang Yani , Zhang Runguang , Wang Guoliang , Zhang Youlin
Published: December 2021

Pseudomonas syringaepv Actinidiae (P. syringae) is a common pathogen causing plant diseases. Limoli proved that its strong pathogenicity is closely related to biofilm state. As a natural bacteriostatic agent with broad-spectrum bactericidal properties, juglone can be used as a substitute for synthetic bacteriostatic agents. To explore the antibacterial mechanism, this study was carried out to examine the inhibitory effect of juglone on cell membrane destruction, abnormal oxidative stress, DNA insertion and biofilm prevention of P. syringae. Results showed that juglone at 20 μg/mL can act against planktogenic P. syringae (107 CFU/mL). Specially, the application of juglone significantly damaged the permeability and integrity of the cell membrane of P. syringae. Additionally, juglone caused abnormal intracellular oxidative stress, and also embedded in genomic DNA, which affected the normal function of the DNA of P. syringae. In addition, environmental scanning electron microscope (ESEM) and other methods showed that juglone effectively restricted the production of extracellular polymers, and then affected the formation of the cell membrane. This study provided a possibility for the development and utilization of natural juglone in plants, especially P. syringae.
A Luminescent Guest@MOF Nanoconfined Composite System for Solid-State Lighting
Authors: Xiong Tao , Zhang Yang , Amin Nader , Tan Jin-Chong
Published: December 2021

A series of rhodamine B (RhB) encapsulated zeolitic imidazolate framework-8 (RhB@ZIF-8) composite nanomaterials with different concentrations of guest loadings have been synthesized and characterized in order to investigate their applicability to solid-state white-light-emitting diodes (WLEDs). The nanoconfinement of the rhodamine B dye (guest) in the sodalite cages of ZIF-8 (host) is supported by fluorescence spectroscopic and photodynamic lifetime data. The quantum yield (QY) of the luminescent RhB@ZIF-8 material approaches unity when the guest loading is controlled at a low level: 1 RhB guest per ~7250 cages. We show that the hybrid (luminescent guest) LG@MOF material, obtained by mechanically mixing a suitably high-QY RhB@ZIF-8 red emitter with a green-emitting fluorescein@ZIF-8 “phosphor” with a comparably high QY, could yield a stable, intensity tunable, near-white light emission under specific test conditions described. Our results demonstrate a novel LG@MOF composite system exhibiting a good combination of photophysical properties and photostability, for potential applications in WLEDs, photoswitches, bioimaging and fluorescent sensors.
Microalgae as a Potential Functional Ingredient: Evaluation of the Phytochemical Profile, Antioxidant Activity and In-Vitro Enzymatic Inhibitory Effect of Different Species
Authors: Vieira Marta Vinha , Turkiewicz Igor Piotr , Tkacz Karolina , Fuentes-Grünewald Claudio , Pastrana Lorenzo M. , Fuciños Pablo , Wojdyło Aneta , Nowicka Paulina
Published: December 2021

The functional food market has been in a state of constant expansion due to the increasing awareness of the impact of the diet on human health. In the search for new natural resources that could act as a functional ingredient for the food industry, microalgae represent a promising alternative, considering their high nutritional value and biosynthesis of numerous bioactive compounds with reported biological properties. In the present work, the phytochemical profile, antioxidant activity, and enzymatic inhibitory effect aiming at different metabolic disorders (Alzheimer’s disease, Type 2 diabetes, and obesity) were evaluated for the species Porphyridium purpureum, Chlorella vulgaris, Arthorspira platensis, and Nannochloropsis oculata. All the species presented bioactive diversity and important antioxidant activity, demonstrating the potential to be used as functional ingredients. Particularly, P. purpureum and N. oculata exhibited higher carotenoid and polyphenol content, which was reflected in their superior biological effects. Moreover, the species P. purpureum exhibited remarkable enzymatic inhibition for all the analyses.
In Search of Small Molecules That Selectively Inhibit MBOAT4
Authors: Murzinski Emily S. , Saha Ishika , Ding Hui , Strugatsky David , Hollibaugh Ryan A. , Liu Haixia , Tontonoz Peter , Harran Patrick G.
Published: December 2021

Ghrelin is a 28-residue peptide hormone produced by stomach P/D1 cells located in oxyntic glands of the fundus mucosa. Post-translational octanoylation of its Ser-3 residue, catalyzed by MBOAT4 (aka ghrelin O-acyl transferase (GOAT)), is essential for the binding of the hormone to its receptor in target tissues. Physiological roles of acyl ghrelin include the regulation of food intake, growth hormone secretion from the pituitary, and inhibition of insulin secretion from the pancreas. Here, we describe a medicinal chemistry campaign that led to the identification of small lipopeptidomimetics that inhibit GOAT in vitro. These molecules compete directly for substrate binding. We further describe the synthesis of heterocyclic inhibitors that compete at the acyl coenzyme A binding site.
In Silico Investigation of the Biological Implications of Complex DNA Damage with Emphasis in Cancer Radiotherapy through a Systems Biology Approach
Authors: Pavlopoulou Athanasia , Asfa Seyedehsadaf , Gioukakis Evangelos , Mavragani Ifigeneia V. , Nikitaki Zacharenia , Takan Işıl , Pouget Jean-Pierre , Harrison Lynn , Georgakilas Alexandros G.
Published: December 2021

Different types of DNA lesions forming in close vicinity, create clusters of damaged sites termed as “clustered/complex DNA damage” and they are considered to be a major challenge for DNA repair mechanisms resulting in significant repair delays and induction of genomic instability. Upon detection of DNA damage, the corresponding DNA damage response and repair (DDR/R) mechanisms are activated. The inability of cells to process clustered DNA lesions efficiently has a great impact on the normal function and survival of cells. If complex lesions are left unrepaired or misrepaired, they can lead to mutations and if persistent, they may lead to apoptotic cell death. In this in silico study, and through rigorous data mining, we have identified human genes that are activated upon complex DNA damage induction like in the case of ionizing radiation (IR) and beyond the standard DNA repair pathways, and are also involved in cancer pathways, by employing stringent bioinformatics and systems biology methodologies. Given that IR can cause repair resistant lesions within a short DNA segment (a few nm), thereby augmenting the hazardous and toxic effects of radiation, we also investigated the possible implication of the most biologically important of those genes in comorbid non-neoplastic diseases through network integration, as well as their potential for predicting survival in cancer patients.
Effects of Malted Rice Amazake on Constipation Symptoms and Gut Microbiota in Children and Adults with Severe Motor and Intellectual Disabilities: A Pilot Study
Authors: Kageyama Suzumi , Inoue Rikako , Hosomi Koji , Park Jonguk , Yumioka Hitomi , Suka Tomo , Kurohashi Yoshihiro , Teramoto Kazuaki , Syauki A. Yasmin , Doi Miki , Sakaue Haruka , Mizuguchi Kenji , Kunisawa Jun , Irie Yasuyuki
Published: December 2021

Constipation is a frequent complication in patients with severe motor and intellectual disabilities (SMID). The aim of this study was to investigate changes in constipation symptoms and gut microbiota associated with the intake of malted rice amazake, a fermented food in Japan, in patients with SMID. Ten patients consumed the test food for six weeks, and their physical condition, dietary and medication status, and constipation assessment scale (CAS) were investigated. Comprehensive fecal microbiome analysis using the 16S rRNA sequence method was performed. The results showed a significant decrease in CAS, and a significant increase in Lactobacillales and decrease in Escherichia-Shigella after consuming malted rice amazake. To investigate the difference in the effects of malted rice amazake consumption, based on the characteristics of the original gut microbiota, the patients were grouped according to the similarity of their gut microbiota before the intervention; Firmicutes-rich Group 1 (n = 5), Actinobacteria-rich Group 2 (n = 4), and Proteobacteria-rich Group 3 (n = 1). The CAS decreased in Groups 1 and 2. The relative abundance of Bifidobacterium showed an increasing tendency both overall and in Group 1, but it was originally higher in Group 2. Our results suggest that malted rice amazake consumption reduces constipation symptoms and simultaneously changes the gut microbiota, but the changes may vary depending on the original composition of the gut microbiota.
Effects of a 3-Week Hospital-Controlled Very-Low-Calorie Diet in Severely Obese Patients
Authors: Ožvald Ivan , Božičević Dragan , Duh Lidija , Vinković Vrček Ivana , Pavičić Ivan , Domijan Ana-Marija , Milić Mirta
Published: December 2021

Although a very-low-calorie diet (VLCD) is considered safe and has demonstrated benefits among other types of diets, data are scarce concerning its effects on improving health and weight loss in severely obese patients. As part of the personalized weight loss program developed at the Duga Resa Special Hospital for Extended Treatment, Croatia, we evaluated anthropometric, biochemical, and permanent DNA damage parameters (assessed with the cytochalasin B-blocked micronucleus cytome assay—CBMN) in severely obese patients (BMI ≥ 35 kg m−2) after 3-weeks on a 567 kcal, hospital-controlled VLCD. This is the first study on the permanent genomic (in)stability in such VLCD patients. VLCDs caused significant decreases in weight (loss), parameters of the lipid profile, urea, insulin resistance, and reduced glutathione (GSH). Genomic instability parameters were lowered by half, reaching reference values usually found in the healthy population. A correlation was found between GSH decrease and reduced DNA damage. VLCDs revealed susceptible individuals with remaining higher DNA damage for further monitoring. In a highly heterogeneous group (class II and III in obesity, differences in weight, BMI, and other categories) consisting of 26 obese patients, the approach demonstrated its usefulness and benefits in health improvement, enabling an individual approach to further monitoring, diagnosis, treatment, and risk assessment based on changing anthropometric/biochemical VLCD parameters, and CBMN results.
High Adherence to the Food Pyramid’s Recommendations Avoids the Risk of Insufficient Nutrient Intake among Farmers in Peri-Urban Kenya
Authors: Kishino Madoka , Hida Azumi , Hara Kenta , Mungai David Nguatha , Opiyo Rose Okoyo , Matsuda Hirotaka , Tada Yuki , Ishikawa-Takata Kazuko , Irie Kenji , Morimoto Yasuyuki
Published: December 2021

This study aimed to investigate whether the Kenyan Food Pyramid (FP) can evaluate excess or insufficient nutrient intake. Participants were farmers (56 men and 64 women, aged 18–60 years) in Wangige Village, Kiambu County—a peri-urban area of Kenya. Cross-sectional data were collected for demographic characteristics, physical measurements, and 2-day and 24-h dietary recalls. The average adherence level to the FP (hereafter, “FP score”) was 25.0 out of 50.0, with a minimum and maximum of 14.1 and 41.5, respectively. Energy and protein % energy ratio were significantly higher (p for trend < 0.05) in the higher FP score group. A higher FP score was also associated with a higher energy-adjusted micronutrient intake, and it was more likely to meet nutrient requirements. However, the higher FP score group had a higher risk of excess sodium intake (p for trend < 0.001). The Kenyan FP could be a useful tool for avoiding the risk of insufficient nutrient intake, but not for avoiding high energy and sodium intake. It is necessary to include appropriate evaluations to limit energy, sugar, and salt. Food groups and recommendations of the FP should be optimised according to the dietary environment of the target population so as to promote their health.
Hepatitis C Vaccination: Where We Are and Where We Need to Be
Authors: Manne Vignan , Ryan John , Wong Jonathan , Vengayil Gayatri , Basit Syed Abdul , Gish Robert G.
Published: December 2021

The hepatitis C virus (HCV) is a common cause of chronic liver disease and liver cancer worldwide. Despite advances in curative therapies for HCV, the incidence of new infections is not decreasing at the expected rate to hit the World Health Organization (WHO) target for the elimination of HCV by 2030. In fact, there are still more new cases of infection in the United States and worldwide than are being cured. The reasons for the rise in new cases include poor access to care and the opioid epidemic. The clinical burden of HCV requires a multimodal approach to eradicating the infection. Vaccination would be an excellent tool to prevent incidence of new infections; however, the genetic diversity of HCV and its ability to generate quasispecies within an infected host make creating a broadly reactive vaccine difficult. Multiple vaccine candidates have been identified, but to date, there has not been a target that has led to a broadly reactive vaccine, though several of the candidates are promising. Additionally, the virus is very difficult to culture and testing candidates in humans or chimpanzees is ethically challenging. Despite the multiple barriers to creating a vaccine, vaccination still represents an important tool in the fight against HCV.
Fungal Species Causing Maize Leaf Blight in Different Agro-Ecologies in India
Authors: Singh Vimla , Lakshman Dilip K. , Roberts Daniel P. , Ismaiel Adnan , Abhishek Alok , Kumar Shrvan , Hooda Karambir S.
Published: December 2021

Foliar diseases of maize cause severe economic losses in India and around the world. The increasing severity of maize leaf blight (MLB) over the past ten years necessitates rigorous identification and characterization of MLB-causing pathogens from different maize production zones to ensure the success of resistance breeding programs and the selection of appropriate disease management strategies. Although Bipolaris maydis is the primary pathogen causing MLB in India, other related genera such as Curvularia, Drechslera, and Exserohilum, and a taxonomically distant genus, Alternaria, are known to infect maize in other countries. To investigate the diversity of pathogens associated with MLB in India, 350 symptomatic leaf samples were collected between 2016 and 2018, from 20 MLB hotspots in nine states representing six ecological zones where maize is grown in India. Twenty representative fungal isolates causing MLB symptoms were characterized based on cultural, pathogenic, and molecular variability. Internal Transcribed Spacer (ITS) and glyceraldehyde-3-phosphate dehydrogenase (GADPH) gene sequence-based phylogenies showed that the majority of isolates (13/20) were Bipolaris maydis. There were also two Curvularia papendorfii isolates, and one isolate each of Bipolaris zeicola, Curvularia siddiquii, Curvularia sporobolicola, an unknown Curvularia sp. isolate phylogenetically close to C. graminicola, and an Alternaria sp. isolate. The B. zeicola, the aforesaid four Curvularia species, and the Alternaria sp. are the first reports of these fungi causing MLB in India. Pathogenicity tests on maize plants showed that isolates identified as Curvularia spp. and Alternaria sp. generally caused more severe MLB symptoms than those identified as Bipolaris spp. The diversity of fungi causing MLB, types of lesions, and variation in disease severity by different isolates described in this study provide baseline information for further investigations on MLB disease distribution, diagnosis, and management in India.
Assessment of Food and Waterborne Viral Outbreaks by Using Field Epidemiologic, Modern Laboratory and Statistical Methods—Lessons Learnt from Seven Major Norovirus Outbreaks in Finland
Authors: Polkowska Aleksandra , Räsänen Sirpa , Nuorti Pekka , Maunula Leena , Jalava Katri
Published: December 2021

Seven major food- and waterborne norovirus outbreaks in Western Finland during 2014–2018 were re-analysed. The aim was to assess the effectiveness of outbreak investigation tools and evaluate the Kaplan criteria. We summarised epidemiological and microbiological findings from seven outbreaks. To evaluate the Kaplan criteria, a one-stage meta-analysis of data from seven cohort studies was performed. The case was defined as a person attending an implicated function with diarrhoea, vomiting or two other symptoms. Altogether, 22% (386/1794) of persons met the case definition. Overall adjusted, 73% of norovirus patients were vomiting, the mean incubation period was 44 h (4 h to 4 days) and the median duration of illness was 46 h. As vomiting was a more common symptom in children (96%, 143/149) and diarrhoea among the elderly (92%, 24/26), symptom and age presentation should drive hypothesis formulation. The Kaplan criteria were useful in initial outbreak assessments prior to faecal results. Rapid food control inspections enabled evidence-based, public-health-driven risk assessments. This led to probability-based vehicle identification and aided in resolving the outbreak event mechanism rather than implementing potentially ineffective, large-scale public health actions such as the withdrawal of extensive food lots. Asymptomatic food handlers should be ideally withdrawn from high-risk work for five days instead of the current two days. Food and environmental samples often remain negative with norovirus, highlighting the importance of research collaborations. Electronic questionnaire and open-source novel statistical programmes provided time and resource savings. The public health approach proved useful within the environmental health area with shoe leather field epidemiology, combined with statistical analysis and mathematical reasoning.
Migalastat Treatment in a Kidney-Transplanted Patient with Fabry Disease and N215S Mutation: The First Case Report
Authors: Di Stefano Valeria , Mancarella Marta , Camporeale Antonia , Regalia Anna , Ferraresi Marta , Pisaniello Marco , Cassinerio Elena , Pieruzzi Federico , Motta Irene
Published: December 2021

Fabry disease is a rare X-linked lysosomal storage disorder caused by mutations in the GLA gene, leading to deficient α-galactosidase A activity and, consequently, to glycosphingolipid accumulation in a wide variety of cells. Fabry disease due to N215S (c.644A>G, p.Asn215Ser) missense mutation usually results in a late-onset phenotype presenting with isolated cardiac involvement. We herein present the case of a patient with N215S mutation with cardiac involvement, namely left ventricular hypertrophy and ventricular arrhythmias, and end-stage renal disease requiring kidney transplantation. To the best of our knowledge, this is the first report of a kidney-transplanted Fabry patient treated with oral pharmacologic chaperone migalastat.
Glycyrrhiza glabra (Licorice): A Comprehensive Review on Its Phytochemistry, Biological Activities, Clinical Evidence and Toxicology
Authors: Wahab Shadma , Annadurai Sivakumar , Abullais Shahabe Saquib , Das Gotam , Ahmad Wasim , Ahmad Md Faruque , Kandasamy Geetha , Vasudevan Rajalakshimi , Ali Md Sajid , Amir Mohd
Published: December 2021

There are more than 30 species of Glycyrrhiza genus extensively spread worldwide. It was the most prescribed herb in Ancient Egyptian, Roman, Greek, East China, and the West from the Former Han era. There are various beneficial effects of licorice root extracts, such as treating throat infections, tuberculosis, respiratory, liver diseases, antibacterial, anti-inflammatory, and immunodeficiency. On the other hand, traditional medicines are getting the attraction to treat many diseases. Therefore, it is vital to screen the medicinal plants to find the potential of new compounds to treat chronic diseases such as respiratory, cardiovascular, anticancer, hepatoprotective, etc. This work comprehensively reviews ethnopharmacological uses, phytochemistry, biological activities, clinical evidence, and the toxicology of licorice, which will serve as a resource for future clinical and fundamental studies. An attempt has been made to establish the pharmacological effect of licorice in different diseases. In addition, the focus of this review article is on the molecular mechanism of licorice extracts and their four flavonoids (isoliquiritigenin, liquiritigenin, lichalocone, and glabridin) pharmacologic activities. Licorice could be a natural alternative for current therapy to exterminate new emerging disorders with mild side effects. This review will provide systematic insights into this ancient drug for further development and clinical use.
Silicon Dioxide Nanoparticles Induce Innate Immune Responses and Activate Antioxidant Machinery in Wheat Against Rhizoctonia solani
Authors: Abdelrhim Abdelrazek , Mazrou Yasser , Nehela Yasser , Atallah Osama , El-Ashmony Ranya , Dawood Mona
Published: December 2021

The phytopathogenic basidiomycetous fungus, Rhizoctonia solani, has a wide range of host plants including members of the family Poaceae, causing damping-off and root rot diseases. In this study, we biosynthesized spherical-shaped silicon dioxide nanoparticles (SiO2 NPs; sized between 9.92 and 19.8 nm) using saffron extract and introduced them as a potential alternative therapeutic solution to protect wheat seedlings against R. solani. SiO2 NPs showed strong dose-dependent fungistatic activity on R. solani, and significantly reduced mycelial radial growth (up to 100% growth reduction), mycelium fresh and dry weight, and pre-, post-emergence damping-off, and root rot severities. Moreover, the impact of SiO2 NPs on the growth of wheat seedlings and their potential mechanism (s) for disease suppression was deciphered. SiO2 NPs application also improved the germination, vegetative growth, and vigor indexes of infected wheat seedlings which indicates no phytotoxicity on treated wheat seedlings. Moreover, SiO2 NPs enhanced the content of the photosynthetic pigments (chlorophylls and carotenoids), induced the accumulation of defense-related compounds (particularly salicylic acid), and alleviated the oxidative stress via stimulation of both enzymatic (POD, SOD, APX, CAT, and PPO) and non-enzymatic (phenolics and flavonoids) antioxidant defense machinery. Collectively, our findings demonstrated the potential therapeutic role of SiO2 NPs against R. solani infection via the simultaneous activation of a multilayered defense system to suppress the pathogen, neutralize the destructive effect of ROS, lipid peroxidation, and methylglyoxal, and maintain their homeostasis within R. solani-infected plants.
Divergence in Corn Mycorrhizal Colonization Patterns Due to Organic Treatment
Authors: Pop-Moldovan Victoria , Vârban Rodica , Corcoz Larisa , Pleșa Anca , Stoian Vlad , Vidican Roxana
Published: December 2021

Excessive application of chemical fertilizers and other agrochemicals can cause large imbalances in soils and agricultural ecosystems. In this context, mycorrhizae represent a viable solution to mitigate these negative effects. Arbuscular mycorrhizae are vital symbionts due to the multiple benefits they bring to both crops and the entire agroecosystem. The main purpose of this study was to observe whether differentiated fertilization has an influence on mycorrhizal colonization patterns in corn. Observed frequencies and intensities of colonization varied widely between phenophases and treatments, with 20% variation for frequency and 14% for intensity, which implies the constant development of both partners during the vegetation period. Arbuscules and vesicles were present in all development stages, but the overall mean was lower than 4% for arbuscules and 1% for vesicles in the analyzed root fragments. Intensity was highly correlated with frequency of colonization compared with arbuscules, where the coefficient was 0.54, and vesicles, with a coefficient of 0.16. Both PCA and NMDS provided good graphical solutions, with a high resolution due to explained variance and good spatial position of vectors. The use of mycorrhizal maps permits the full exploration of colonization patterns and fungal strategy, and the assessment of mycorrhizae-free areas. For the untreated variant, the strategy was oriented toward a longitudinal colonization followed by an irregular development of hyphae with multiple non-colonized areas. Treatment acts to stimulate the appearance of mycorrhizal spots, which further develop radially.
Determination of the Thermodynamic Parameters of the Pyrolysis Process of Post-Consumption Thermoplastics by Non-Isothermal Thermogravimetric Analysis
Authors: Palmay Paul , Puente Cesar , Barzallo Diego , Bruno Joan Carles
Published: December 2021

Currently, the pyrolysis process is an important technology for the final treatment of plastic waste worldwide. For this reason, knowing in detail the chemical process and the thermodynamics that accompany cracking reactions is of utmost importance. The present study aims to determine the thermodynamic parameters of the degradation process of conventional thermoplastics (polystyrene (PS), polyethylene terephthalate (PET), high-density polyethylene (HDPE), polypropylene (PP) and polyvinyl chloride (PVC)) from the study of their chemical kinetics by thermogravimetric analysis (TG). Non-isothermal thermogravimetry was performed at three heating rates from room temperature to 550 °C with an inert nitrogen atmosphere with a flow of 20 mL min−1. Once the TG data is obtained, an analysis is carried out with the isoconversional models of Friedman (FR), Kissinger–Akahira–Sunose (KAS), and Flynn–Wall–Ozawa (FWO) in order to determine the one that best fits the experimental data, and with this, the calculation of the activation energy and the pre-exponential factor is performed. The validation of the model was carried out using the correlation factor, determining that the KAS model is the one that best adjusts for the post-consumer thermoplastic degradation process at the three heating rates. With the use of the kinetic parameters, the variation of the Gibbs free energy is determined in each of the cases, where it is necessary that for structures containing aromatic groups a lower energy is presented, which implies a relative ease of degradation compared to the linear structures.
Self-Healable and Recyclable Biomass-Derived Polyurethane Networks through Carbon Dioxide Immobilization
Authors: Baek Seohyun , Lee Juhyen , Kim Hyunwoo , Cha Inhwan , Song Changsik
Published: December 2021

Due to growing environmental issues, research on carbon dioxide (CO2) use is widely conducted and efforts are being made to produce useful materials from biomass-derived resources. However, polymer materials developed by a combined strategy (i.e., both CO2-immobilized and biomass-derived) are rare. In this study, we synthesized biomass-derived poly(carbonate-co-urethane) (PCU) networks using CO2-immobilized furan carbonate diols (FCDs) via an ecofriendly method. The synthesis of FCDs was performed by directly introducing CO2 into a biomass-derived 2,5-bis(hydroxymethyl)furan. Using mechanochemical synthesis (ball-milling), the PCU networks were effortlessly prepared from FCDs, erythritol, and diisocyanate, which were then hot-pressed into films. The thermal and thermomechanical properties of the PCU networks were thoroughly characterized by thermogravimetric analysis, differential scanning calorimetry, dynamic (thermal) mechanical analysis, and using a rheometer. The self-healing and recyclable properties of the PCU films were successfully demonstrated using dynamic covalent bonds. Interestingly, transcarbamoylation (urethane exchange) occurred preferentially as opposed to transcarbonation (carbonate exchange). We believe our approach presents an efficient means for producing sustainable polyurethane copolymers using biomass-derived and CO2-immobilized diols.
Limited-Stage Small-Cell Lung Cancer: Current Progress and the Next Frontier
Authors: Toh Tzen S. , Lok Benjamin H.
Published: December 2021

Limited-stage (LS) small-cell lung cancer (SCLC) is defined as disease confined to a tolerable radiation portal without extrathoracic metastases. Despite clinical research over two decades, the prognosis of LS-SCLC patients remains poor. The current standard of care for LS-SCLC patients is concurrent platinum-based chemotherapy with thoracic radiotherapy (RT). Widespread heterogeneity on the optimal radiation dose and fractionation regimen among physicians highlights the logistical challenges of administering BID regimens. Prophylactic cranial irradiation (PCI) is recommended to patients following a good initial response to chemoradiation due to improved overall survival from historical trials and the propensity for LS-SCLC to recur with brain metastases. However, PCI utilization is being debated due to the greater availability of magnetic resonance imaging (MRI) and data in extensive-stage SCLC regarding close MRI surveillance in lieu of PCI while spurring novel RT techniques, such as hippocampal-avoidance PCI. Additionally, novel treatment combinations incorporating targeted small molecule therapies and immunotherapies with or following radiation for LS-SCLC have seen recent interest and some concepts are being investigated in clinical trials. Here, we review the landscape of progress, limitations, and challenges for LS-SCLC including current standard of care, novel radiation techniques, and the integration of novel therapeutic strategies for LS-SCLC.
Land Surface Temperature Retrieval from Fengyun-3D Medium Resolution Spectral Imager II (FY-3D MERSI-II) Data with the Improved Two-Factor Split-Window Algorithm
Authors: Du Wenhui , Qin Zhihao , Fan Jinlong , Zhao Chunliang , Huang Qiuyan , Cao Kun , Abbasi Bilawal
Published: December 2021

Land surface temperature (LST) is an essential parameter widely used in environmental studies. The Medium Resolution Spectral Imager II (MERSI-II) boarded on the second generation Chinese polar-orbiting meteorological satellite, Fengyun-3D (FY-3D), provides a new opportunity for LST retrieval at a spatial resolution of 250 m that is higher than that of the already widely used Moderate Resolution Imaging Spectrometer (MODIS) LST data of 1000 m. However, there is no operational LST product from FY-3D MERSI-II data available for free access. Therefore, in this study, we developed an improved two-factor split-window algorithm (TFSWA) of LST retrieval from this data source as it has two thermal-infrared (TIR) bands. The essential coefficients of the TFSWA algorithm have been carefully and precisely estimated for the FY-3D MERSI-II TIR thermal bands. A new approach for estimating land surface emissivity has been developed using the ASTER Global Emissivity Database (ASTER GED) and the International Geosphere-Biosphere Program (IGBP) data. A model to estimate the atmospheric water vapor content (AWVC) from the three atmospheric water vapor absorption bands (bands 16, 17, and 18) has been developed as AWVC has been recognized as the most important factor determining the variation of AT. Using MODTRAN 5.2, the equations for the AT estimate from the retrieved AWVC were established. In addition, the AT of the pixels at the far edge of FY-3D MERSI-II data may be strongly affected by the increase of the optical path. Viewing zenith angle (VZA) correction equations were proposed in the study to correct this effect on AT estimation. Field data from four stations were applied to validate the improved TFSWA in the study. Cross-validation with MODIS LST (MYD11) was also conducted to evaluate the improved TFSWA. The cross-validation result indicates that the FY-3D MERSI-II LST from the improved TFSWA are comparable with MODIS LST while the correlation coefficients between FY-3D MERSI-II LST and MODIS LST over the Mid-East China region are in the range of 0.84~0.98 for different seasons and land cover types. Validation with 318 field LST samples indicates that the average MAE and R2 of the scenes at the four stations are about 1.97 K and 0.98, respectively. Thus, it could be concluded that the improved TFSWA developed in the study can be a good algorithm for LST retrieval from FY-3D MERSI-II data with acceptable accuracy.
Towards Routine Mapping of Crop Emergence within the Season Using the Harmonized Landsat and Sentinel-2 Dataset
Authors: Gao Feng , Anderson Martha C. , Johnson David M. , Seffrin Robert , Wardlow Brian , Suyker Andy , Diao Chunyuan , Browning Dawn M.
Published: December 2021

Crop emergence is a critical stage for crop development modeling, crop condition monitoring, and biomass accumulation estimation. Green-up dates (or the start of the season) detected from remote sensing time series are related to, but generally lag, crop emergence dates. In this paper, we refine the within-season emergence (WISE) algorithm and extend application to five Corn Belt states (Iowa, Illinois, Indiana, Minnesota, and Nebraska) using routine harmonized Landsat and Sentinel-2 (HLS) data from 2018 to 2020. Green-up dates detected from the HLS time series were assessed using field observations and near-surface measurements from PhenoCams. Statistical descriptions of green-up dates for corn and soybeans were generated and compared to county-level planting dates and district- to state-level crop emergence dates reported by the National Agricultural Statistics Service (NASS). Results show that emergence dates for corn and soybean can be reliably detected within the season using the HLS time series acquired during the early growing season. Compared to observed crop emergence dates, green-up dates from HLS using WISE were ~3 days later at the field scale (30-m). The mean absolute difference (MAD) was ~7 days and the root mean square error (RMSE) was ~9 days. At the state level, the mean differences between median HLS green-up date and median crop emergence date were within 2 days for 2018–2020. At this scale, MAD was within 4 days, and RMSE was less than 5 days for both corn and soybeans. The R-squares were 0.73 and 0.87 for corn and soybean, respectively. The 2019 late emergence of crops in Corn Belt states (1–4 weeks to five-year average) was captured by HLS green-up date retrievals. This study demonstrates that routine within-season mapping of crop emergence/green-up at the field scale is practicable over large regions using operational satellite data. The green-up map derived from HLS during the growing season provides valuable information on spatial and temporal variability in crop emergence that can be used for crop monitoring and refining agricultural statistics used in broad-scale modeling efforts.