bluuebunny/crossref_metadata_2025 huggingface dataset:
Tomatidine Represses Invasion and Migration of Human Osteosarcoma U2OS and HOS Cells by Suppression of Presenilin 1 and c-Raf–MEK–ERK Pathway
Authors: Hsieh Min-Hong , Yang Jia-Sin , Lin Renn-Chia , Hsieh Yi-Hsien , Yang Shun-Fa , Chang Horng-Rong , Lu Ko-Hsiu
Published: January 2020

Osteosarcoma, which is the most prevalent malignant bone tumor, is responsible for the great majority of bone cancer-associated deaths because of its highly metastatic potential. Although tomatidine is suggested to serve as a chemosensitizer in multidrug-resistant tumors, the anti-metastatic effect of tomatidine in osteosarcoma is still unknown. Here, we tested the hypothesis that tomatidine suppresses migration and invasion, features that are associated with metastatic process in human osteosarcoma cells and also investigate its underlying pathway. Tomatidine, up to 100 μM, without cytotoxicity, inhibited the invasion and migration capabilities of human osteosarcoma U2OS and HOS cells and repressed presenilin 1 (PS-1) expression of U2OS cells. After the knockdown of PS-1, U2OS and HOS cells’ biological behaviors of cellular invasion and migratory potential were significantly reduced. While tomatidine significantly decreased the phosphorylation of c-Raf, mitogen/extracellular signal-regulated kinase (MEK), and extracellular signal-regulated protein kinase (ERK)1/2 in U2OS cells, no obvious influences on p-Jun N-terminal kinase, p38, and Akt, including their phosphorylation, were observed. In ERK 1 silencing U2 OS cells, tomatidine further enhanced the decrease of their migratory potential and invasive activities. We conclude that both PS-1 derived from U2OS and HOS cells and the c-Raf–MEK–ERK pathway contribute to cellular invasion and migration and tomatidine could inhibit the phenomenons. These findings indicate that tomatidine might be a potential candidate for anti-metastasis treatment of human osteosarcoma.
Editorial to the Special Issue—“Technology for Natural Products Research”
Authors: Duggan Brendan M.
Published: January 2020

Natural product research continues to be a productive source of unusual chemistry, producing novel compounds for biomedical applications and, increasingly, sustainably providing commercially useful compounds [...]
The Generation of the Oxidant Agent of a Mononuclear Nonheme Fe(II) Biomimetic Complex by Oxidative Decarboxylation. A DFT Investigation
Authors: Parise Angela , Muraca Maria Costanza , Russo Nino , Toscano Marirosa , Marino Tiziana
Published: January 2020

The oxidative decarboxylation of the iron(II) α-hydroxy acid (mandelic acid) complex model, biomimetic of Rieske dioxygenase, has been investigated at the density functional level. The explored mechanism sheds light on the role of the α-hydroxyl group on the dioxygen activation. The potential energy surfaces have been explored in different electronic spin states. The rate-determining step of the process is the proton transfer. The oxidative decarboxylation preferentially takes place on the quintet state.
Heteroaromatic N-Oxides in Asymmetric Catalysis: A Review
Authors: Wrzeszcz Zuzanna , Siedlecka Renata
Published: January 2020

An increasing interest in the synthesis and use of optically active pyridine N-oxides as chiral controllers for asymmetric reactions has been observed in the last few years. Chiral heteroaromatic N-oxides can work as powerful electron-pair donors, providing suitable electronic environments in the transition state formed within the reaction. The nucleophilicity of the oxygen atom in N-oxides, coupled with a high affinity of silicon to oxygen, represent ideal properties for the development of synthetic methodology based on nucleophilic activation of organosilicon reagents. The application of chiral N-oxides as efficient organocatalysts in allylation, propargylation, allenylation, and ring-opening of meso-epoxides, as well as chiral ligands for metal complexes catalyzing Michael addition or nitroaldol reaction, can also be found in the literature. This review deals with stereoselective applications of N-oxides, and how the differentiating properties are correlated with their structure. It contains more recent results, covering approximately the last ten years. All the reported examples have been divided into five classes, according to the chirality elements present in their basic molecular frameworks.
Magnetic Driven Nanocarriers for pH-Responsive Doxorubicin Release in Cancer Therapy
Authors: Nogueira João , Soares Sofia F. , Amorim Carlos O. , Amaral João S. , Silva Cláudia , Martel Fátima , Trindade Tito , Daniel-da-Silva Ana L.
Published: January 2020

Doxorubicin is one of the most widely used anti-cancer drugs, but side effects and selectivity problems create a demand for alternative drug delivery systems. Herein we describe a hybrid magnetic nanomaterial as a pH-dependent doxorubicin release carrier. This nanocarrier comprises magnetic iron oxide cores with a diameter of 10 nm, enveloped in a hybrid material made of siliceous shells and ĸ-carrageenan. The hybrid shells possess high drug loading capacity and a favorable drug release profile, while the iron oxide cores allows easy manipulation via an external magnetic field. The pH responsiveness was assessed in phosphate buffers at pH levels equivalent to those of blood (pH 7.4) and tumor microenvironment (pH 4.2 and 5). The nanoparticles have a loading capacity of up to 12.3 wt.% and a release profile of 80% in 5 h at acidic pH versus 25% at blood pH. In vitro drug delivery tests on human breast cancer and non-cancer cellular cultures have shown that, compared to the free drug, the loaded nanocarriers have comparable antiproliferative effect but a less intense cytotoxic effect, especially in the non-cancer cell line. The results show a clear potential for these new hybrid nanomaterials as alternative drug carriers for doxorubicin.
Infrared and 2-Dimensional Correlation Spectroscopy Study of the Effect of CH3NH3PbI3 and CH3NH3SnI3 Photovoltaic Perovskites on Eukaryotic Cells
Authors: Quaroni Luca , Benmessaoud Iness , Vileno Bertrand , Horváth Endre , Forró László
Published: January 2020

We studied the effect of the exposure of human A549 and SH-SY5Y cell lines to aqueous solutions of organic/inorganic halide perovskites CH3NH3PbI3 (MAPbI3) and CH3NH3SnI3 (MASnI3) at the molecular level by using Fourier transform infrared microspectroscopy. We monitored the infrared spectra of some cells over a few days following exposure to the metals and observed the spectroscopic changes dominated by the appearance of a strong band at 1627 cm−1. We used Infrared (IR) mapping to show that this change was associated with the cell itself or the cellular membrane. It is unclear whether the appearance of the 1627 cm−1 band and heavy metal exposure are related by a direct causal relationship. The spectroscopic response of exposure to MAPbI3 and MASnI3 was similar, indicating that it may arise from a general cellular response to stressful environmental conditions. We used 2D correlation spectroscopy (2DCOS) analysis to interpret spectroscopic changes. In a novel application of the method, we demonstrated the viability of 2DCOS for band assignment in spatially resolved spectra. We assigned the 1627 cm−1 band to the accumulation of an abundant amide or amine containing compound, while ruling out other hypotheses. We propose a few tentative assignments to specific biomolecules or classes of biomolecules, although additional biochemical characterization will be necessary to confirm such assignments.
Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells
Authors: Cheng Zheng-Yu , Hsiao Yung-Ting , Huang Yi-Ping , Peng Shu-Fen , Huang Wen-Wen , Liu Kuo-Ching , Hsia Te-Chun , Way Tzong-Der , Chung Jing-Gung
Published: January 2020

Casticin was obtained from natural plants, and it has been shown to exert biological functions; however, no report concerns the induction of DNA damage and repair in human lung cancer cells. The objective of this study was to investigate the effects and molecular mechanism of casticin on DNA damage and repair in human lung cancer A549 cells. Cell viability was determined by flow cytometric assay. The DNA damage was evaluated by 4’,6-diamidino-2-phenylindole (DAPI) staining and electrophoresis which included comet assay and DNA gel electrophoresis. The protein levels associated with DNA damage and repair were analyzed by western blotting. The expression and translocation of p-H2A.X were observed by confocal laser microscopy. Casticin reduced total viable cell number and induced DNA condensation, fragmentation, and damage in A549 cells. Furthermore, casticin increased p-ATM at 6 h and increased p-ATR and BRCA1 at 6–24 h treatment but decreased p-ATM at 24–48 h, as well as decreased p-ATR and BRCA1 at 48 h. Furthermore, casticin decreased p-p53 at 6–24 h but increased at 48 h. Casticin increased p-H2A.X and MDC1 at 6–48 h treatment. In addition, casticin increased PARP (cleavage) at 6, 24, and 48 h treatment, DNA-PKcs and MGMT at 48 h in A549 cells. Casticin induced the expressions and nuclear translocation of p-H2AX in A549 cells by confocal laser microscopy. Casticin reduced cell number through DNA damage and condensation in human lung cancer A549 cells.
Chemical Composition and the Cytotoxic, Antimicrobial, and Anti-Inflammatory Activities of the Fruit Peel Essential Oil from Spondias pinnata (Anacardiaceae) in Xishuangbanna, Southwest China
Authors: Li Ren , Yang Jing-Jing , Song Xing-Zhen , Wang Yuan-Fei , Corlett Richard , Xu You-Kai , Hu Hua-Bin
Published: January 2020

Spondias pinnata (Linn. f.) Kurz (Anacardiaceae) is widely distributed in tropical Asia, where it is commonly used as a vegetable and fruit, and is attracting increasing research attention. In this study, we investigated the chemical composition and the cytotoxic, antimicrobial, and anti-inflammatory activities of the fruit peel essential oil of S. pinnata (EOSP), which has been consumed as a medicine and condiment in Xishuangbanna, southwest China. A total of 40 components were identified by Gas Chromatography/Mass spectrometry (GC-MS), representing 95.19% of the EOSP, with furfural (17.14%), α-terpineol (13.09%), and ethyl benzoate (9.05%) as the main constituents. EOSP has moderate cytotoxic activity against five cancer cells and obvious antimicrobial activity against five pathogenic strains. In particular, EOSP (Minimal Inhibitory and Fungicidal Concentration, MIC and MFC, 16‒32 µg/mL) showed a 32-times higher inhibition effect against Aspergillus fumigatus than the positive control Tigecycline (MIC and MBC 512‒1024 µg/mL). EOSP also showed strong anti-inflammatory activity by significantly inhibiting nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW 264.7 cell lines at 0.08‰, with no effect on cell viability. These bioactivities of S. pinnata fruit peel validate its traditional uses and suggest that it could be a new source of natural antimicrobial and anti-inflammatory agents for food or medical industries.
Antifungal Styryloquinolines as Candida albicans Efflux Pump Inhibitors: Styryloquinolines are ABC Transporter Inhibitors
Authors: Cieslik Wioleta , Szczepaniak Joanna , Krasowska Anna , Musiol Robert
Published: January 2020

Styrylquinolines are heterocyclic compounds that are known for their antifungal and antimicrobial activity. Metal complexation through hydroxyl groups has been claimed to be a plausible mechanism of action for these types of compounds. A series of novel structures with protected hydroxyl groups have been designed and synthesized to verify the literature data. Their antifungal activity against wild-type Candida albicans strain and mutants with silenced efflux pumps activity has been determined. Combinations with fluconazole revealed synergistic interactions that were dependent on the substitution pattern. These results open a new route for designing active antifungal agents on a styrylquinoline scaffold.
AT-MSCs Antifibrotic Activity is Improved by Eugenol through Modulation of TGF-β/Smad Signaling Pathway in Rats
Authors: Fathy Moustafa , Okabe Motonori , Saad Eldien Heba M. , Yoshida Toshiko
Published: January 2020

For hepatic failure, stem cell transplantation has been chosen as an alternative therapy, especially for mesenchymal stem cells (MSCs). The aim of this study was to investigate the effect of eugenol (EUG) on the in vivo antifibrotic activity of adipose tissue-derived MSCs (AT-MSCs) and the underlying mechanism. After characterization of MSCs, rats were divided into five groups, Group 1 (normal control), Group 2 (CCl4), Group 3 (CCl4 + AT-MSCs), Group 4 (CCl4 + EUG) and Group 5 (CCl4 + AT-MSCs + EUG). Biochemical and histopathological investigations were performed. Furthermore, expression of type 1 collagen, α-SMA, TGF-β1, Smad3 and P-Smad3 was estimated. Compared to the single treatment with AT-MSCs, the combination treatment of the fibrotic rats with AT-MSCs and EUG significantly improved the plasma fibrinogen concentration, IL-10 level and proliferating cell nuclear antigen expression, and also significantly decreased the serum levels of liver enzymes, IL-6, IL-1β, TNF-α, type III collagen, hyaluronic acid, hydroxyproline and the TGF-β growth factor. Furthermore, the combination treatment significantly decreased the hepatic expression of fibrotic markers genes (Type 1 collagen and α-SMA) and proteins (α-SMA, TGF-β1 and phospho-Smad3) more than the treatment with AT-MSCs alone. We demonstrated that the combination treatment with EUG and AT-MSCs strongly inhibited the advancement of CCl4-induced hepatic fibrosis, compared with AT-MSCs alone, through TGF-β/Smad pathway inhibition. This approach is completely novel, so more investigations are necessary to improve our perception of the underlying molecular mechanisms accountable for the effects of EUG on the antifibrotic potential of AT-MSCs.
Review of the Post-IR IRSL Dating Protocols of K-Feldspar
Authors: Zhang Junjie , Li Sheng-Hua
Published: January 2020

Compared to quartz, the infrared stimulated luminescence (IRSL) of K-feldspar saturates at higher dose, which has great potential for extending the dating limit. However, dating applications with K-feldspar has been hampered due to anomalous fading of the IRSL signal. The post-IR IRSL (pIRIR) signal of K-feldspar stimulated at a higher temperature after a prior low-temperature IR stimulation has significantly lower fading rate. Different dating protocols have been proposed with the pIRIR signals and successful dating applications have been made. In this study, we review the development of various pIRIR dating protocols, and compare their performance in estimating the equivalent dose (De). Standard growth curves (SGCs) of the pIRIR signals of K-feldspar are introduced. Single-grain K-feldspar pIRIR dating is presented and the existing problems are discussed.
Intrinsic Control in Defects Density for Improved ZnO Nanorod-Based UV Sensor Performance
Authors: Rana Abu ul Hassan Sarwar , Shaikh Shoyebmohamad F. , Al-Enizi Abdullah M. , Agyeman Daniel Adjei , Ghani Faizan , Nah In Wook , Shahid Areej
Published: January 2020

Hitherto, most research has primarily focused on improving the UV sensor efficiency via surface treatments and by stimulating the ZnO nanorod (ZNR) surface Schottky barriers. However, to the best of our knowledge, no study has yet probed the intrinsic crystal defect generation and its effects on UV sensor efficiency. In this study, we undertake this task by fabricating an intrinsic defect-prone hydrothermally grown ZNRs (S1), Ga-doped ZNRs (S2), and defect-free microwave-assisted grown ZNRs (S3). The defect states were recognized by studying X-ray diffraction and photoluminescence characteristics. The large number of crystal defects in S1 and S2 had two pronged disadvantages. (1) Most of the UV light was absorbed by the defect traps and the e–h pair generation was compromised. (2) Mobility was directly affected by the carrier–carrier scattering and phonon scattering processes. Hence, the overall UV sensor efficiency was compromised based on the defect-induced mobility-response model. Considering the facts, defect-free S3 exhibited the best UV sensor performance with the highest on/off ratio, the least impulse response time, the highest recombination time, and highest gain-induced responsivity to 368 nm UV light, which was desired of an efficient passive metal oxide-based UV sensor. Our results were compared with the recently published results.
The Role of the Laser-Induced Oxide Layer in the Formation of Laser-Induced Periodic Surface Structures
Authors: Florian Camilo , Déziel Jean-Luc , Kirner Sabrina , Siegel Jan , Bonse Jörn
Published: January 2020

Laser-induced periodic surface structures (LIPSS) are often present when processing solid targets with linearly polarized ultrashort laser pulses. The different irradiation parameters to produce them on metals, semiconductors and dielectrics have been studied extensively, identifying suitable regimes to tailor its properties for applications in the fields of optics, medicine, fluidics and tribology, to name a few. One important parameter widely present when exposing the samples to the high intensities provided by these laser pulses in air environment, that generally is not considered, is the formation of a superficial laser-induced oxide layer. In this paper, we fabricate LIPSS on a layer of the oxidation prone hard-coating material chromium nitride in order to investigate the impact of the laser-induced oxide layer on its formation. A variety of complementary surface analytic techniques were employed, revealing morphological, chemical and structural characteristics of well-known high-spatial frequency LIPSS (HSFL) together with a new type of low-spatial frequency LIPSS (LSFL) with an anomalous orientation parallel to the laser polarization. Based on this input, we performed finite-difference time-domain calculations considering a layered system resembling the geometry of the HSFL along with the presence of a laser-induced oxide layer. The simulations support a scenario that the new type of LSFL is formed at the interface between the laser-induced oxide layer and the non-altered material underneath. These findings suggest that LSFL structures parallel to the polarization can be easily induced in materials that are prone to oxidation.
The Effect of Caffeine and Trifluralin on Chromosome Doubling in Wheat Anther Culture
Authors: Broughton Sue , Castello Marieclaire , Liu Li , Killen Julie , Hepworth Anna , O’Leary Rebecca
Published: January 2020

Challenges for wheat doubled haploid (DH) production using anther culture include genotype variability in green plant regeneration and spontaneous chromosome doubling. The frequency of chromosome doubling in our program can vary from 14% to 80%. Caffeine or trifluralin was applied at the start of the induction phase to improve early genome doubling. Caffeine treatment at 0.5 mM for 24 h significantly improved green plant production in two of the six spring wheat crosses but had no effect on the other crosses. The improvements were observed in Trojan/Havoc and Lancer/LPB14-0392, where green plant numbers increased by 14% and 27% to 161 and 42 green plants per 30 anthers, respectively. Caffeine had no significant effect on chromosome doubling, despite a higher frequency of doubling in several caffeine treatments in the first experiment (67–68%) compared to the control (56%). In contrast, trifluralin significantly improved doubling following a 48 h treatment, from 38% in the control to 51% and 53% in the 1 µM and 3 µM trifluralin treatments, respectively. However, trifluralin had a significant negative effect on green plant regeneration, declining from 31.8 green plants per 20 anthers (control) to 9–25 green plants per 20 anthers in the trifluralin treatments. Further work is required to identify a treatment regime with caffeine and/or anti-mitotic herbicides that consistently increases chromosome doubling in wheat without reducing green plant regeneration.
Cationic Copolymerization of Isobutylene with 4-Vinylbenzenecyclobutylene: Characteristics and Mechanisms
Authors: Chen Zhifei , Li Shuxin , Shang Yuwei , Huang Shan , Wu Kangda , Guo Wenli , Wu Yibo
Published: January 2020

A random copolymer of isobutylene (IB) and 4-vinylbenzenecyclobutylene (4-VBCB) was synthesized by cationic polymerization at −80 °C using 2-chloro-2,4,4-trimethylpentane (TMPCl) as initiator. The laws of copolymerization were investigated by changing the feed quantities of 4-VBCB. The molecular weight of the copolymer decreased, and its molecular weight distribution (MWD) increased with increasing 4-VBCB content. We proposed a possible copolymerization mechanism behind the increase in the chain transfer reaction to 4-VBCB with increasing of feed quantities of 4-VBCB. The thermal properties of the copolymers were studied by solid-phase heating and crosslinking. After crosslinking, the decomposition and glass transition temperatures (Tg) of the copolymer increased, the network structure that formed did not break when reheated, and the mechanical properties remarkably improved.
Influence of PVAc/PVA Hydrolysis on Additive Surface Activity
Authors: Squillace Ophélie , Fong Rebecca , Shepherd Oliver , Hind Jasmine , Tellam James , Steinke Nina-Juliane , Thompson Richard L.
Published: January 2020

This aims to establish design rules for the influence of complex polymer matrices on the surface properties of small molecules. Here, we consider the dependence of the surface behaviour of some model additives on polymer matrix hydrophobicity. With stoichiometric control over hydrolysis, we generate systematic changes in matrix chemistry from non-polar, hydrophobic PVAc to its hydrolysed and hydrophilic analogue, PVA. With the changing degree of hydrolysis (DH), the behaviour of additives can be switched in terms of compatibility and surface activity. Sorbitol, a polar sugar-alcohol of inherently high surface energy, blooms to the surface of PVAc, forming patchy domains on surfaces. With the increasing DH of the polymer matrix, its surface segregation decreases to the point where sorbitol acts as a homogeneously distributed plasticiser in PVA. Conversely, and despite its low surface energy, octanoic acid (OA) surprisingly causes the increased wettability of PVAc. We attribute these observations to the high compatibility of OA with PVAc and its ability to reorient upon exposure to water, presenting a hydrophilic COOH-rich surface. The surfactant sodium dodecyl sulfate (SDS) does not show such a clear dependence on the matrix and formed wetting layers over a wide range of DH. Interestingly, SDS appears to be most compatible with PVAc at intermediate DH, which is consistent with the amphiphilic nature of both species under these conditions. Thus, we show that the prediction of the segregation is not simple and depends on multiple factors including hydrophobicity, compatibility, blockiness, surface energy, and the mobility of the components.
(1S,2S)-Cyclohexane-1,2-diamine-based Organosilane Fibres as a Powerful Tool Against Pathogenic Bacteria
Authors: Máková Veronika , Holubová Barbora , Tetour David , Brus Jiří , Řezanka Michal , Rysová Miroslava , Hodačová Jana
Published: January 2020

An urgent need to find an effective solution to bacterial resistance is pushing worldwide research for highly effective means against this threat. Newly prepared hybrid organosilane fibres consisting of a (1S,2S)-cyclohexane-1,2-diamine derivative, interconnected in the fibre network via covalent bonds, were fully characterised via different techniques, including FTIR, TGA-FTIR, SEM-EDS, and solid-state NMR. Fibrous samples were successfully tested against two types of pathogenic bacterial strains, namely Staphylococcus aureus, and Pseudomonas aeruginosa. The obtained results, showing >99.9% inhibition against Staphylococcus aureus and Pseudomonas aeruginosa in direct contact compared to the control, may help particularly in case of infections, where there is an urgent need to treat the infection in direct contact. From this point of view, the above-mentioned fibrous material may find application in wound healing. Moreover, this new material has a positive impact on fibroblasts viability.
Chemical Modification and Processing of Chitin for Sustainable Production of Biobased Electrolytes
Authors: Latifi Meriem , Ahmad Azizan , Kaddami Hamid , Hasyareeda Hassan Nur , Dieden Reiner , Habibi Youssef
Published: January 2020

In the present work we report on the development of a novel and sustainable electrolyte based on chitin. Chitin biopolymer was carboxymethylated in simple, mild, and green conditions in order to fine-tune the final properties of the electrolyte. To this end, chitin was modified for various reaction times, while the molar ratio of the reagents, e.g., sodium hydroxide and monochloroacetic acid, was maintained fixed. The resulting chitin derivatives were characterized using various techniques. Under optimized conditions, modified chitin derivatives exhibiting a distinct degree of carboxymethylation and acetylation were obtained. Structural features, morphology, and properties are discussed in relation to the chemical structure of the chitin derivatives. For electrolyte applications, the ionic conductivity increased by three magnitudes from 10−9 S·cm−1 for unmodified chitin to 10−6 S·cm−1 for modified chitin with the highest degree of acetylation. Interestingly, the chitin derivatives formed free-standing films with and without the addition of up to 60% of ionic liquid, the ionic conductivity of the obtained solid electrolyte system reaching the value of 10−3 S·cm−1.
Damage Evolution and Fracture Events Sequence Analysis of Core-Shell Nanoparticle Modified Bone Cements by Acoustic Emission Technique
Authors: Pacheco-Salazar O.F. , Wakayama Shuichi , Can-Herrera L.A. , Dzul-Cervantes M.A.A. , Ríos-Soberanis C.R. , Cervantes-Uc J.M.
Published: January 2020

In this research, damage in bone cements that were prepared with core-shell nanoparticles was monitored during four-point bending tests through an analysis of acoustic emission (AE) signals. The core-shell structure consisted of poly(butyl acrylate) (PBA) as rubbery core and methyl methacrylate/styrene copolymer (P(MMA-co-St)) as a glassy shell. Furthermore, different core-shell ratios 20/80, 30/70, 40/60, and 50/50 were prepared and incorporated into the solid phase of the bone cement formulation at 5, 10, and 15 wt %, respectively. The incorporation of a rubbery phase into the bone cement formulation decreased the bending strength and bending modulus. The AE technique revealed that the nanoparticles play an important role on the fracture mechanism of the bone cement, since a higher amount of AE signals (higher amplitude and energy) were obtained from bone cements that were prepared with the nanoparticles in comparison with those without nanoparticles (the reference bone cement). The SEM examination of the fracture surfaces revealed that all of the bone cement formulations exhibited stress whitening, which arises from the development of crazes before the crack propagation. Finally, the use of the AE technique and the fracture surface analysis by SEM enabled insight into the fracture mechanisms that are presented during four-point bending test of the bone cement containing nanoparticles.
Special Issue “Top-down Proteomics: In Memory of Dr Alfred Yergey”. Alfred Linwood Yergey, III, 17 September 1941–27 May 2018
Authors: Coorssen Jens R. , Padula Matthew P.
Published: January 2020

Please see J [...]
‘Moderate Islam’ Made in the United Arab Emirates: Public Diplomacy and the Politics of Containment
Authors: Kourgiotis Panos
Published: January 2020

This essay addresses the ideological utilization of religion in the international relations of the United Arab Emirates during the Arab Spring and beyond. By referring to the theoretical framework of public diplomacy and analyzing UAE regional and domestic attitudes, this essay intends to examine the politics of ‘moderate Islam’ in line with: (a) the monarchy’s nation building visions for the 21st century; (b) its national rebranding strategies; (c) its geopolitical empowerment in the Gulf and the Middle East. Throughout our analysis, it is argued that even though ‘moderate Islam’ has been devised for creating ‘soft power’, it serves ‘sharp power’ as well. As will become obvious, this has been mainly the case as far as the containment of Political Islam is concerned.
A Review of the Literature on the Lower-Mobility Parallel Manipulators of 3-UPU or 3-URU Type
Authors: Di Gregorio Raffaele
Published: January 2020

Various 3-UPU architectures feature two rigid bodies connected to one another through three kinematic chains (limbs) of universal–prismatic–universal (UPU) type. They were first proposed in the last decade of the 20th century and have animated discussions among researchers for more-or-less two decades. Such discussions brought to light many features of lower-mobility parallel manipulators (PMs) that were unknown until then. The discussions also showed that such architectures may be sized into translational PMs, parallel wrists, or even reconfigurable (metamorphic) PMs. Even though commercial robots with these architectures have not yet been built, the interest in them remains. Consequently, a review of the literature on these architectures, highlighting their contribution to the progress of lower-mobility PM design, is still of interest for the scientific community. This paper aims at presenting a critical review of the results that have been obtained up until now.
Evaluation of Coherent and Incoherent Landslide Detection Methods Based on Synthetic Aperture Radar for Rapid Response: A Case Study for the 2018 Hokkaido Landslides
Authors: Jung Jungkyo , Yun Sang-Ho
Published: January 2020

Damage mapping using Synthetic Aperture Radar (SAR) imagery has been studied in recent decades to support rapid response to natural disasters. Many researches have been developing coherent and incoherent change detection. However, their performances can vary depending on the types of the damages, the characteristics of the scatterers and the corresponding capability of algorithms. In particular, the coherence-based methods have been used as promising detectors over urban areas where high coherences are observed, but their detection accuracies still remain controversial over the area where low coherences are mainly observed such as the 2018 Hokkaido landslides. In order to understand the characteristics of landslide (damage) detectors for low-coherence areas and find an alternative and complementary method, we designed the coherence difference, coherence normalized difference, log-ratio, intensity correlation difference, and normalized differences of the intensity correlation assuming limited availability of dataset, and also developed multi-temporal algorithms using the coherence, intensity, and intensity correlation. They were tested and evaluated using multiple polygons extracted from aerial photos. We were able to observe that the multi-temporal intensity correlation method has the potential to detect the landslides over the low coherence region and all types of land uses.
Flood Detection and Susceptibility Mapping Using Sentinel-1 Remote Sensing Data and a Machine Learning Approach: Hybrid Intelligence of Bagging Ensemble Based on K-Nearest Neighbor Classifier
Authors: Shahabi Himan , Shirzadi Ataollah , Ghaderi Kayvan , Omidvar Ebrahim , Al-Ansari Nadhir , Clague John J. , Geertsema Marten , Khosravi Khabat , Amini Ata , Bahrami Sepideh , Rahmati Omid , Habibi Kyoumars , Mohammadi Ayub , Nguyen Hoang , Melesse Assefa M. , Ahmad Baharin Bin , Ahmad Anuar
Published: January 2020

Mapping flood-prone areas is a key activity in flood disaster management. In this paper, we propose a new flood susceptibility mapping technique. We employ new ensemble models based on bagging as a meta-classifier and K-Nearest Neighbor (KNN) coarse, cosine, cubic, and weighted base classifiers to spatially forecast flooding in the Haraz watershed in northern Iran. We identified flood-prone areas using data from Sentinel-1 sensor. We then selected 10 conditioning factors to spatially predict floods and assess their predictive power using the Relief Attribute Evaluation (RFAE) method. Model validation was performed using two statistical error indices and the area under the curve (AUC). Our results show that the Bagging–Cubic–KNN ensemble model outperformed other ensemble models. It decreased the overfitting and variance problems in the training dataset and enhanced the prediction accuracy of the Cubic–KNN model (AUC=0.660). We therefore recommend that the Bagging–Cubic–KNN model be more widely applied for the sustainable management of flood-prone areas.
Detection of Methane Emission from a Local Source Using GOSAT Target Observations
Authors: Kuze Akihiko , Kikuchi Nobuhiro , Kataoka Fumie , Suto Hiroshi , Shiomi Kei , Kondo Yutaka
Published: January 2020

Emissions of atmospheric methane (CH4), which greatly contributes to radiative forcing, have larger uncertainties than those for carbon dioxide (CO2). The Thermal And Near-infrared Sensor for carbon Observation Fourier-Transform Spectrometer (TANSO-FTS) onboard the Greenhouse gases Observing SATellite (GOSAT) launched in 2009 has demonstrated global grid observations of the total column density of CO2 and CH4 from space, and thus reduced uncertainty in the global flux estimation. In this paper, we present a case study on local CH4 emission detection from a single-point source using an available series of GOSAT data. By modifying the grid observation pattern, the pointing mechanism of TANSO-FTS targets a natural gas leak point at Aliso Canyon in Southern California, where the clear-sky frequency is high. To enhance local emission estimates, we retrieved CO2 and CH4 partial column-averaged dry-air mole fractions of the lower troposphere (XCO2 (LT) and XCH4 (LT)) by simultaneous use of both sunlight reflected from Earth’s surface and thermal emissions from the atmosphere. The time-series data of Aliso Canyon showed a large enhancement that decreased with time after its initial blowout, compared with reference point data and filtered with wind direction simulated by the Weather Research and Forecasting (WRF) model.
Continuous Tracking of Targets for Stereoscopic HFSWR Based on IMM Filtering Combined with ELM
Authors: Zhang Ling , Mao Dongwei , Niu Jiong , Wu Q. M. Jonathan , Ji Yonggang
Published: January 2020

High frequency surface wave radar (HFSWR) plays an important role in marine surveillance on account of its ability to provide wide-range early warning detection. However, vessel target track breakages are common in large-scale marine monitoring, which limits the continuous tracking ability of HFSWR. The following are the possible reasons for track fracture: highly maneuverable vessels, dense channels, target occlusion, strong clutter/interference, long sampling intervals, and low detection probabilities. To solve this problem, we propose a long-term continuous tracking method for multiple targets with stereoscopic HFSWR based on an interacting multiple model extended Kalman filter (IMMEKF) combined with an extreme learning machine (ELM). When the trajectory obtained by IMMEKF breaks, a new section of the track will start on the basis of the observation data. For multiple-target tracking, a number of broken tracks can be obtained by IMMEKF tracking. Then the ELM classifies the segments from the same vessel by extracting different features including average velocity, average curvature, ratio of the arc length to the chord length, and wavelet coefficient. Both the simulation and the field experiment results validate the method presented here, showing that this method can achieve long-term continuous tracking for multiple vessels, with an average correct track segment association rate of over 91.2%, which is better than the tracking performance of conventional algorithms, especially when the vessels are in dense channels and strong clutter/interference area.
Grouping-Based Time-Series Model for Monitoring of Fall Peak Coloration Dates Using Satellite Remote Sensing Data
Authors: Zhou Qu , Sun Xianghan , Tian Liqiao , Li Jian , Li Wenkai
Published: January 2020

Accurate monitoring of plant phenology is vital to effective understanding and prediction of the response of vegetation ecosystems to climate change. Satellite remote sensing is extensively employed to monitor vegetation phenology. However, fall phenology, such as peak foliage coloration, is less well understood compared with spring phenological events, and is mainly determined using the vegetation index (VI) time-series. Each VI only emphasizes a single vegetation property. Thus, selecting suitable VIs and taking advantage of multiple spectral signatures to detect phenological events is challenging. In this study, a novel grouping-based time-series approach for satellite remote sensing was proposed, and a wide range of spectral wavelengths was considered to monitor the complex fall foliage coloration process with simultaneous changes in multiple vegetation properties. The spatial and temporal scale effects of satellite data were reduced to form a reliable remote sensing time-series, which was then divided into groups, namely pre-transition, transition and post-transition groups, to represent vegetation dynamics. The transition period of leaf coloration was correspondingly determined to divisions with the smallest intra-group and largest inter-group distances. Preliminary results using a time-series of Moderate Resolution Imaging Spectroradiometer (MODIS) data from 2002 to 2013 at the Harvard Forest (spatial scale: ~3500 m; temporal scale: ~8 days) demonstrated that the method can accurately determine the coloration period (correlation coefficient: 0.88; mean absolute difference: 3.38 days), and that the peak coloration periods displayed a shifting trend to earlier dates. The grouping-based approach shows considerable potential in phenological monitoring using satellite time-series.
The Use of the CORINE Land Cover (CLC) Database for Analyzing Urban Sprawl
Authors: Cieślak Iwona , Biłozor Andrzej , Szuniewicz Karol
Published: January 2020

Urban sprawl is generally defined as the urbanization of space adjacent to a city, which results from that city’s development. The discussed phenomenon involves land development, mainly agricultural land, in the proximity of cities, the development of infrastructure, and an increase in the number of residents who rely on urban services and commute to work in the city. Urban sprawl generates numerous problems which, in the broadest sense, result from the difficulty in identifying the boundaries of the central urban unit and the participation of local inhabitants, regardless of their actual place of residence, in that unit’s functional costs. These problems are associated not only with tax collection rights but with difficulties in measuring the extent of urban sprawl in research and local governance. The aim of this study was to analyze the applicability of the CORINE Land Cover (CLC) database for monitoring urbanization processes, including the dynamic process of urban sprawl. Polish cities with county rights, i.e., cities that implement independent spatial planning policies, were analyzed in the study to determine the pattern of urban sprawl in various types of cities. Buffer zones composed of municipalities that are directly adjacent to the central urban unit were mapped around the analyzed cities. The study proposes a novel method for measuring the extent of suburbanization with the use of the CLC database and Geographic Information System (GIS) tools. The developed method relies on the overgrowth of urbanization (OU) index calculated based on CLC data. The OU index revealed differences in the rate of urbanization in three groups of differently sized Polish cities. The analysis covered two periods: 2006–2012 and 2012–2018, and it revealed that urban sprawl in the examined cities proceeded in an unstable manner over time. The results of the present study indicate that the CLC database is a reliable source of information about urbanization processes.
Photon-Detection-Probability Simulation Method for CMOS Single-Photon Avalanche Diodes
Authors: Hsieh Chin-An , Tsai Chia-Ming , Tsui Bing-Yue , Hsiao Bo-Jen , Lin Sheng-Di
Published: January 2020

Single-photon avalanche diodes (SPADs) in complementary metal-oxide-semiconductor (CMOS) technology have excellent timing resolution and are capable to detect single photons. The most important indicator for its sensitivity, photon-detection probability (PDP), defines the probability of a successful detection for a single incident photon. To optimize PDP is a cost- and time-consuming task due to the complicated and expensive CMOS process. In this work, we have developed a simulation procedure to predict the PDP without any fitting parameter. With the given process parameters, our method combines the process, the electrical, and the optical simulations in commercially available software and the calculation of breakdown trigger probability. The simulation results have been compared with the experimental data conducted in an 800-nm CMOS technology and obtained a good consistence at the wavelength longer than 600 nm. The possible reasons for the disagreement at the short wavelength have been discussed. Our work provides an effective way to optimize the PDP of a SPAD prior to its fabrication.
Assessment of Laying Hens’ Thermal Comfort Using Sound Technology
Authors: Du Xiaodong , Carpentier Lenn , Teng Guanghui , Liu Mulin , Wang Chaoyuan , Norton Tomas
Published: January 2020

Heat stress is one of the most important environmental stressors facing poultry production and welfare worldwide. The detrimental effects of heat stress on poultry range from reduced growth and egg production to impaired health. Animal vocalisations are associated with different animal responses and can be used as useful indicators of the state of animal welfare. It is already known that specific chicken vocalisations such as alarm, squawk, and gakel calls are correlated with stressful events, and therefore, could be used as stress indicators in poultry monitoring systems. In this study, we focused on developing a hen vocalisation detection method based on machine learning to assess their thermal comfort condition. For extraction of the vocalisations, nine source-filter theory related temporal and spectral features were chosen, and a support vector machine (SVM) based classifier was developed. As a result, the classification performance of the optimal SVM model was 95.1 ± 4.3% (the sensitivity parameter) and 97.6 ± 1.9% (the precision parameter). Based on the developed algorithm, the study illustrated that a significant correlation existed between specific vocalisations (alarm and squawk call) and thermal comfort indices (temperature-humidity index, THI) (alarm-THI, R = −0.414, P = 0.01; squawk-THI, R = 0.594, P = 0.01). This work represents the first step towards the further development of technology to monitor flock vocalisations with the intent of providing producers an additional tool to help them actively manage the welfare of their flock.
Processes of Sub-Citizenship: Neoliberal Statecrafting ‘Citizens,’ ‘Non-Citizens,’ and Detainable ‘Others’
Authors: Rung Daile Lynn
Published: January 2020

Increasingly, scholars are exploring the politics of migration and the shifting terrain of citizenship from a critical mobilities perspective. To contribute to these discussions, in this paper, I explore how processes of sub-citizenship occur as nation-states craft immigration, citizenship, and border securitization policies and practices. I argue that complex and shifting processes of sub-citizenship largely occur through the nation-state’s production of ‘insiders’ (‘citizens’) and ‘outsiders’ (‘non-citizens’). As a nascent attempt to introduce sub-citizenship, I draw upon recent high-profile cases of family separation, abuse, and neglect experienced by children with ‘illegal migrant’ status in the United States and Australia. Under the international nation-state system and the neoliberal globalization paradigm, the border policing powers of nation-states are primed to expand and intensify processes of sub-citizenship. Those at lower levels of the sub-citizen hierarchy are at risk of experiencing various forms of state-led violence, including deportation, detention, and torture.
Modulation of Vaccine-Induced HIV-1-Specific Immune Responses by Co-Electroporation of PD-L1 Encoding DNA
Authors: Tannig Pierre , Peter Antonia Sophia , Lapuente Dennis , Klessing Stephan , Damm Dominik , Tenbusch Matthias , Überla Klaus , Temchura Vladimir
Published: January 2020

The importance of a balanced TH1/TH2 humoral immune response against the HIV-1 envelope protein (Env) for antibody-mediated HIV-1 control is increasingly recognized. However, there is no defined vaccination strategy to raise it. Since immune checkpoints are involved in the induction of adoptive immunity and their inhibitors (monoclonal antibodies) are licensed for cancer therapy, we investigated the effect of checkpoint blockade after HIV-1 genetic vaccination on enhancement and modulation of antiviral antibody responses. By intraperitoneal administration of checkpoint antibodies in mice we observed an induction of anti-drug antibodies which may interfere with immunomodulation by checkpoint inhibitors. Therefore, we blocked immune checkpoints locally by co-electroporation of DNA vaccines encoding the active soluble ectodomains of programmed cell death protein-1 (PD-1) or its ligand (PD-L1), respectively. Plasmid-encoded immune checkpoints did not elicit a detectable antibody response, suggesting no interference with their immunomodulatory effects. Co-electroporation of a HIV-1 DNA vaccine formulation with soluble PD-L1 ectodomain increased HIV-1 Env-specific TH1 CD4 T cell and IgG2a antibody responses. The overall antibody response was hereby shifted towards a more TH1/TH2 balanced subtype pattern. These findings indicate that co-electroporation of soluble checkpoint ectodomains together with DNA-based vaccines has modulatory effects on vaccine-induced immune responses that could improve vaccine efficacies.
Effects of Normative Aging on Eye Movements during Reading
Authors: Paterson Kevin B. , McGowan Victoria A. , Warrington Kayleigh L. , Li Lin , Li Sha , Xie Fang , Chang Min , Zhao Sainan , Pagán Ascensión , White Sarah J. , Wang Jingxin
Published: January 2020

Substantial progress has been made in understanding the mostly detrimental effects of normative aging on eye movements during reading. This article provides a review of research on aging effects on eye movements during reading for different writing systems (i.e., alphabetic systems like English compared to non-alphabetic systems like Chinese), focused on appraising the importance of visual and cognitive factors, considering key methodological issues, and identifying vital questions that need to be addressed and topics for further investigation.