bluuebunny/crossref_metadata_2025 huggingface dataset:
Revealing the Role of High-Density Lipoprotein in Colorectal Cancer
Authors: Zeljkovic Aleksandra , Vekic Jelena , Mihajlovic Marija , Gojkovic Tamara , Vladimirov Sandra , Zeljkovic Dejan , Spasojevic-Kalimanovska Vesna , Trifunovic Bratislav
Published: March 2021

Colorectal cancer (CRC) is a highly prevalent malignancy with multifactorial etiology, which includes metabolic alterations as contributors to disease development. Studies have shown that lipid status disorders are involved in colorectal carcinogenesis. In line with this, previous studies have also suggested that the serum high-density lipoprotein cholesterol (HDL-C) level decreases in patients with CRC, but more recently, the focus of investigations has shifted toward the exploration of qualitative properties of HDL in this malignancy. Herein, a comprehensive overview of available evidences regarding the putative role of HDL in CRC will be presented. We will analyze existing findings regarding alterations of HDL-C levels but also HDL particle structure and distribution in CRC. In addition, changes in HDL functionality in this malignancy will be discussed. Moreover, we will focus on the genetic regulation of HDL metabolism, as well as the involvement of HDL in disturbances of cholesterol trafficking in CRC. Finally, possible therapeutic implications related to HDL will be presented. Given the available evidence, future studies are needed to resolve all raised issues concerning the suggested protective role of HDL in CRC, its presumed function as a biomarker, and eventual therapeutic approaches based on HDL.
Hypoxia-Induced Alpha-Globin Expression in Syncytiotrophoblasts Mimics the Pattern Observed in Preeclamptic Placentas
Authors: Masoumi Zahra , Erlandsson Lena , Hansson Eva , Magnusson Mattias , Mezey Eva , Hansson Stefan R.
Published: March 2021

Preeclampsia (PE) is a pregnancy disorder associated with placental dysfunction and elevated fetal hemoglobin (HbF). Early in pregnancy the placenta harbors hematopoietic stem and progenitor cells (HSPCs) and is an extramedullary source of erythropoiesis. However, globin expression is not unique to erythroid cells and can be triggered by hypoxia. To investigate the role of the placenta in increasing globin levels previously reported in PE, flow cytometry, histological and immunostaining and in situ analyses were used on placenta samples and ex vivo explant cultures. Our results indicated that in PE pregnancies, placental HSPC homing and erythropoiesis were not affected. Non-erythroid alpha-globin mRNA and protein, but not gamma-globin, were detected in syncytiotrophoblasts and stroma of PE placenta samples. Similarly, alpha-globin protein and mRNA were upregulated in normal placenta explants cultured in hypoxia. The upregulation was independent of HIF1 and NRF2, the two main candidates of globin transcription in non-erythroid cells. Our study is the first to demonstrate alpha-globin mRNA expression in syncytiotrophoblasts in PE, induced by hypoxia. However, gamma-globin was only expressed in erythrocytes. We conclude that alpha-globin, but not HbF, is expressed in placental syncytiotrophoblasts in PE and may contribute to the pathology of the disease.
Synthesis and Enantioselective Pharmacokinetic/Pharmacodynamic Analysis of New CNS-Active Sulfamoylphenyl Carbamate Derivatives
Authors: Odi Reem , Bibi David , Shusterman Bella , Erenburg Natalia , Shaul Chanan , Supuran Claudiu T. , Nocentini Alessio , Bialer Meir
Published: March 2021

We recently reported a new class of carbamate derivatives as anticonvulsants. Among these, 3-methylpentyl(4-sulfamoylphenyl)carbamate (MSPC) stood out as the most potent compound with ED50 values of 13 mg/kg (i.p.) and 28 mg/kg (p.o.) in the rat maximal electroshock test (MES). 3-Methylpropyl(4-sulfamoylphenyl)carbamate (MBPC), reported and characterized here, is an MSPC analogous compound with two less aliphatic carbon atoms in its structure. As both MSPC and MBPC are chiral compounds, here, we studied the carbonic anhydrase inhibitory and anticonvulsant action of both MBPC enantiomers in comparison to those of MSPC as well as their pharmacokinetic properties. Racemic-MBPC and its enantiomers showed anticonvulsant activity in the rat maximal electroshock (MES) test with ED50 values in the range of 19–39 mg/kg. (R)-MBPC had a 65% higher clearance than its enantiomer and, consequently, a lower plasma exposure (AUC) than (S)-MSBC and racemic-MSBC. Nevertheless, (S)-MBPC had a slightly better brain permeability than (R)-MBPC with a brain-to-plasma (AUC) ratio of 1.32 (S-enantiomer), 1.49 (racemate), and 1.27 (R-enantiomer). This may contribute to its better anticonvulsant-ED50 value. The clearance of MBPC enantiomers was more enantioselective than the brain permeability and MES-ED50 values, suggesting that their anticonvulsant activity might be due to multiple mechanisms of action.
The GRKs Reactome: Role in Cell Biology and Pathology
Authors: Chaudhary Preeti Kumari , Kim Soochong
Published: March 2021

G protein-coupled receptor kinases (GRKs) are protein kinases that function in concert with arrestins in the regulation of a diverse class of G protein-coupled receptors (GPCRs) signaling. Although GRKs and arrestins are key participants in the regulation of GPCR cascades, the complex regulatory mechanisms of GRK expression, its alternation, and their function are not thoroughly understood. Several studies together with the work from our lab in recent years have revealed the critical role of these kinases in various physiological and pathophysiological processes, including cardiovascular biology, inflammation and immunity, neurodegeneration, thrombosis, and hemostasis. A comprehensive understanding of the mechanisms underlying functional interactions with multiple receptor proteins and how these interactions take part in the development of various pathobiological processes may give rise to novel diagnostic and therapeutic strategies. In this review, we summarize the current research linking the role of GRKs to various aspects of cell biology, pathology, and therapeutics, with a particular focus on thrombosis and hemostasis.
Molecular, Cellular and Functional Analysis of TRγ Chain along the European Sea Bass Dicentrarchus labrax Development
Authors: Miccoli Andrea , Guerra Laura , Pianese Valeria , Saraceni Paolo Roberto , Buonocore Francesco , Taddei Anna Rita , Couto Ana , De Wolf Tania , Fausto Anna Maria , Scapigliati Giuseppe , Picchietti Simona
Published: March 2021

In jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide chains, which display an Ig domain-type sequence that is somatically rearranged. γδ T cells have been less extensively characterized than αβ and teleost fish, in particular, suffer from a severe scarcity of data. In this paper, we worked on the well-known model, the European sea bass Dicentrarchus labrax, to broaden the understanding of teleost γδ-T cells. The T cell receptor chain (TR) γ transcript was expressed at a later developmental stage than TRβ, suggesting a layered appearance of fish immune cells, and the thymus displayed statistically-significant higher mRNA levels than any other organ or lymphoid tissue investigated. The polyclonal antibody developed against the TRγ allowed the localization of TRγ-expressing cells in lymphoid organs along the ontogeny. Cell positivity was investigated through flow cytometry and the highest percentage was found in peripheral blood leukocytes, followed by thymus, gut, gills, spleen and head kidney. Numerous TRγ-expressing cells were localized in the gut mucosa, and the immunogold labelling revealed ultrastructural features that are typical of T cells. At last, microalgae-based diet formulations significantly modulated the abundance of TRγ+ cells in the posterior intestine, hinting at a putative involvement in nutritional immunity. From a comparative immunological perspective, our results contribute to the comprehension of the diversity and functionalities of γδ T cells during the development of a commercially relevant marine teleost model.
Airway Surface Liquid pH Regulation in Airway Epithelium Current Understandings and Gaps in Knowledge
Authors: Zajac Miroslaw , Dreano Elise , Edwards Aurelie , Planelles Gabrielle , Sermet-Gaudelus Isabelle
Published: March 2021

Knowledge on the mechanisms of acid and base secretion in airways has progressed recently. The aim of this review is to summarize the known mechanisms of airway surface liquid (ASL) pH regulation and their implication in lung diseases. Normal ASL is slightly acidic relative to the interstitium, and defects in ASL pH regulation are associated with various respiratory diseases, such as cystic fibrosis. Basolateral bicarbonate (HCO3−) entry occurs via the electrogenic, coupled transport of sodium (Na+) and HCO3−, and, together with carbonic anhydrase enzymatic activity, provides HCO3− for apical secretion. The latter mainly involves CFTR, the apical chloride/bicarbonate exchanger pendrin and paracellular transport. Proton (H+) secretion into ASL is crucial to maintain its relative acidity compared to the blood. This is enabled by H+ apical secretion, mainly involving H+/K+ ATPase and vacuolar H+-ATPase that carry H+ against the electrochemical potential gradient. Paracellular HCO3− transport, the direction of which depends on the ASL pH value, acts as an ASL protective buffering mechanism. How the transepithelial transport of H+ and HCO3− is coordinated to tightly regulate ASL pH remains poorly understood, and should be the focus of new studies.
Loss of Fer Jeopardizes Metabolic Plasticity and Mitochondrial Homeostasis in Lung and Breast Carcinoma Cells
Authors: Mehazri Linoy , Shpungin Sally , Bel Shai , Nir Uri
Published: March 2021

Metabolic plasticity is a hallmark of the ability of metastatic cancer cells to survive under stressful conditions. The intracellular Fer kinase is a selective constituent of the reprogramed mitochondria and metabolic system of cancer cells. In the current work, we deciphered the modulatory roles of Fer in the reprogrammed metabolic systems of metastatic, lung (H358), non-small cell lung cancer (NSCLC), and breast (MDA-MB-231), triple-negative breast cancer (TNBC), carcinoma cells. We show that H358 cells devoid of Fer (H358ΔFer), strictly depend on glucose for their proliferation and growth, and fail to compensate for glucose withdrawal by oxidizing and metabolizing glutamine. Furthermore, glucose deficiency caused increased reactive oxygen species (ROS) production and induction of a DNA damage response (DDR), accompanied by the onset of apoptosis and attenuated cell-cycle progression. Analysis of mitochondrial function revealed impaired respiratory and electron transport chain (ETC) complex 1 (comp. I) activity in the Fer-deficient H358ΔFer cells. This was manifested by decreased levels of NAD+ and ATP and relatively low abundance of tricarboxylic acid (TCA) cycle metabolites. Impaired electron transport chain comp. I activity and dependence on glucose were also confirmed in Fer-deficient, MDA-MB-231ΔFer cells. Although both H358ΔFer and MDA-MB-231ΔFer cells showed a decreased aspartate level, this seemed to be compensated by the predominance of pyrimidines synthesis over the urea cycle progression. Notably, absence of Fer significantly impeded the growth of H358ΔFer and MDA-MB-231ΔFer xenografts in mice provided with a carb-deficient, ketogenic diet. Thus, Fer plays a key role in the sustention of metabolic plasticity of malignant cells. In compliance with this notion, targeting Fer attenuates the progression of H358 and MDA-MB-231 tumors, an effect that is potentiated by a glucose-restrictive diet.
Dynamics of Cyclooxygenase-1 Positive Microglia/Macrophage in the Retina of Pathological Model Mice as a Biomarker of the Retinal Inflammatory Diseases
Authors: Makabe Kenichi , Sugita Sunao , Futatsugi Yoko , Takahashi Masayo
Published: March 2021

In an intraocular inflammatory state, microglia residing in the retina become active and migrate inside the retina. In this study, we investigated whether cyclooxygenase-1 (COX-1) expressed by retinal microglia/macrophage can be a biomarker for the diagnosis of retinal diseases. COX-1 was immunopositive in microglia/macrophage and neutrophils, while COX-2 was immunopositive in astrocytes and neurons in the inner layer of normal retina. The number of COX-1 positive cells per section of the retinal tissue was 14 ± 2.8 (mean ± standard deviation) in normal mice, which showed significant increase in the lipopolysaccharide (LPS)-administrated model (62 ± 5.0, p = 8.7 × 10−9). In addition to microglia, we found neutrophils that were positive for COX-1. In the early stage of inflammation in the experimental autoimmune uveoretinitis (EAU), COX-1 positive cells, infiltrating from the ciliary body into the retinal outer nuclear layer, were observed. The number of infiltrating COX-1 positive cells correlated with the severity of EAU. Taken together, the increased number of COX-1 positive microglia/macrophage with morphological changes were observed in the retinas of retinal inflammatory disease models. This suggests that COX-1 can be a marker of disease-related activities of microglia/macrophage, which should be useful for the diagnosis of retinal diseases.
Photocatalytic Activity of Multicompound TiO2/SiO2 Nanoparticles
Authors: Temerov Filipp , Haapanen Janne , Mäkelä Jyrki M. , Saarinen Jarkko J.
Published: March 2021

Multicompound TiO2/SiO2 nanoparticles with a diameter of 50–70 nm were generated using a liquid flame spray (LFS) nanoparticle deposition in a single flame. Here, we study the photocatalytic activity of deposited multicompound nanoparticles in gas-phase via oxidation of acetylene into carbon dioxide that gives new insight about the multicompound nanoparticle morphology. A small addition of SiO2 content of 0.5%, 1.0% and 3.0% significantly suppressed the photocatalytic activity by 33%, 44% and 70%, respectively, whereas 5.0% SiO2 addition completely removed the activity. This may be due to a formation of a thin passivating SiO2 layer on top of the of the TiO2 nanostructures during the LFS nanoparticle deposition. Surface wetting results support this hypothesis with a significant increase in water contact angle as the SiO2 content is increased.
Molecular Characterization of Donacia provosti (Coleoptera: Chrysomelidae) Larval Transcriptome by De Novo Assembly to Discover Genes Associated with Underwater Environmental Adaptations
Authors: Zhan Haixia , Dewer Youssef , Qu Cheng , Yang Shiyong , Luo Chen , Li Liangjun , Li Fengqi
Published: March 2021

Donacia provosti (Fairmaire, 1885) is a major pest of aquatic crops. It has been widely distributed in the world causing extensive damage to lotus and rice plants. Changes in gene regulation may play an important role in adaptive evolution, particularly during adaptation to feeding and living habits. However, little is known about the evolution and molecular mechanisms underlying the adaptation of D. provosti to its lifestyle and living habits. To address this question, we generated the first larval transcriptome of D. provosti. A total of 20,692 unigenes were annotated from the seven public databases and around 18,536 protein-coding genes have been predicted from the analysis of D. provosti transcriptome. About 5036 orthologous cutlers were identified among four species and 494 unique clusters were identified from D. provosti larvae including the visual perception. Furthermore, to reveal the molecular difference between D. provosti and the Colorado potato beetle Leptinotarsa decemlineata, a comparison between CDS of the two beetles was conducted and 6627 orthologous gene pairs were identified. Based on the ratio of nonsynonymous and synonymous substitutions, 93 orthologous gene pairs were found evolving under positive selection. Interestingly, our results also show that there are 4 orthologous gene pairs of the 93 gene pairs were associated with the “mTOR signaling pathway”, which are predicted to be involved in the molecular mechanism of D. provosti adaptation to the underwater environment. This study will provide us with an important scientific basis for building effective prevention and control system of the aquatic leaf beetle Donacia provosti.
Cuticle Hydrocarbons Show Plastic Variation under Desiccation in Saline Aquatic Beetles
Authors: Botella-Cruz María , Velasco Josefa , Millán Andrés , Hetz Stefan , Pallarés Susana
Published: March 2021

In the context of aridification in Mediterranean regions, desiccation resistance and physiological plasticity will be key traits for the persistence of aquatic insects exposed to increasing desiccation stress. Control of cuticular transpiration through changes in the quantity and composition of epicuticular hydrocarbons (CHCs) is one of the main mechanisms of desiccation resistance in insects, but it remains largely unexplored in aquatic ones. We studied acclimation responses to desiccation in adults of two endemic water beetles from distant lineages living in Mediterranean intermittent saline streams: Enochrus jesusarribasi (Hydrophilidae) and Nebrioporus baeticus (Dytiscidae). Cuticular water loss and CHC composition were measured in specimens exposed to a prior non-lethal desiccation stress, allowed to recover and exposed to a subsequent desiccation treatment. E. jesusarribasi showed a beneficial acclimation response to desiccation: pre-desiccated individuals reduced cuticular water loss rate in a subsequent exposure by increasing the relative abundance of cuticular methyl-branched compounds, longer chain alkanes and branched alkanes. In contrast, N. baeticus lacked acclimation capacity for controlling water loss and therefore may have a lower physiological capacity to cope with increasing aridity. These results are relevant to understanding biochemical adaptations to drought stress in inland waters in an evolutionary and ecological context.
The Fossil Record of Elateridae (Coleoptera: Elateroidea): Described Species, Current Problems and Future Prospects
Authors: Kundrata Robin , Packova Gabriela , Prosvirov Alexander S. , Hoffmannova Johana
Published: March 2021

The Elateridae (click-beetles) are the largest family in Elateroidea; however, their relationships, systematics and classification remain unclear. Our understanding of the origin, evolution, palaeodiversity and palaeobiogeography of Elateridae, as well as reconstruction of a reliable time-calibrated phylogeny for the group, are hampered by the lack of detailed knowledge of their fossil record. In this study, we summarize the current knowledge on all described fossil species in Elateridae, including their type material, geographic origin, age, bibliography and remarks on their systematic placement. Altogether, 261 fossil species classified in 99 genera and nine subfamilies are currently listed in this family. The Mesozoic click-beetle diversity includes 143 species, with most of them described from the Jurassic Karatau, and 118 described species are known from the Cenozoic deposits, mainly from the Eocene North American Florissant Formation and European Baltic amber. Available data on the described past diversity of Elateridae suggest that almost all fossil lineages in this group are in urgent need of revision and numerous Mesozoic species might belong to different families. Our study is intended to serve as a comprehensive basis for all subsequent research focused on the click-beetle fossil record.
A Phenome-Wide Association Study (PheWAS) of COVID-19 Outcomes by Race Using the Electronic Health Records Data in Michigan Medicine
Authors: Salvatore Maxwell , Gu Tian , Mack Jasmine A. , Prabhu Sankar Swaraaj , Patil Snehal , Valley Thomas S. , Singh Karandeep , Nallamothu Brahmajee K. , Kheterpal Sachin , Lisabeth Lynda , Fritsche Lars G. , Mukherjee Bhramar
Published: March 2021

Background: We performed a phenome-wide association study to identify pre-existing conditions related to Coronavirus disease 2019 (COVID-19) prognosis across the medical phenome and how they vary by race. Methods: The study is comprised of 53,853 patients who were tested/diagnosed for COVID-19 between 10 March and 2 September 2020 at a large academic medical center. Results: Pre-existing conditions strongly associated with hospitalization were renal failure, pulmonary heart disease, and respiratory failure. Hematopoietic conditions were associated with intensive care unit (ICU) admission/mortality and mental disorders were associated with mortality in non-Hispanic Whites. Circulatory system and genitourinary conditions were associated with ICU admission/mortality in non-Hispanic Blacks. Conclusions: Understanding pre-existing clinical diagnoses related to COVID-19 outcomes informs the need for targeted screening to support specific vulnerable populations to improve disease prevention and healthcare delivery.
Epidemiology and Viral Etiology of Pediatric Immune Thrombocytopenia through Korean Public Health Data Analysis
Authors: Lim Jae Hee , Kim Yu Kyeong , Min So Hyeon , Kim Sang Won , Lee Young Hwan , Lee Jae Min
Published: March 2021

Immune thrombocytopenic purpura (ITP) is prevalent in children aged 2–5 years but may occur in all pediatric age groups. In 50–60% of pediatric patients, ITP is preceded by an upper respiratory tract infection 1–4 weeks before its onset. In this study, the relationship between the development of ITP and viral infections in children was assessed. We analyzed data of 6487 patients aged < 18 years with incident ITP from the Health Insurance Review and Assessment Open Access Big Data Platform (2015 to 2018) and the Korea Disease Control and Prevention Agency. The monthly positive detection rate (PDR) of seven respiratory and four acute diarrhea viruses was calculated. The virus PDR seasonal trend data was analyzed through ARIMA modeling. The ITP diagnostic data and prevalence of viral infection 1 and 2 months prior were analyzed using the Granger test. The overall male to female (M/F) ratio was 1.2, whereas it was 1.4 in the youngest age group (< 1 year). The overall ITP incidence rate was 18.1 per 100,000 person-years. Respiratory syncytial virus, rhinovirus, rotavirus, and astrovirus infections influenced ITP occurrence in children. However, rotavirus infection is positively associated with the etiology of ITP after 1–2 months.
Multicriteria Ship Route Planning Method Based on Improved Particle Swarm Optimization–Genetic Algorithm
Authors: Zhao Wei , Wang Yan , Zhang Zhanshuo , Wang Hongbo
Published: March 2021

With the continuous prosperity and development of the shipping industry, it is necessary and meaningful to plan a safe, green, and efficient route for ships sailing far away. In this study, a hybrid multicriteria ship route planning method based on improved particle swarm optimization–genetic algorithm is presented, which aims to optimize the meteorological risk, fuel consumption, and navigation time associated with a ship. The proposed algorithm not only has the fast convergence of the particle swarm algorithm but also improves the diversity of solutions by applying the crossover operation, selection operation, and multigroup elite selection operation of the genetic algorithm and improving the Pareto optimal frontier distribution. Based on the Pareto optimal solution set obtained by the algorithm, the minimum-navigation-time route, the minimum-fuel-consumption route, the minimum-navigation-risk route, and the recommended route can be obtained. Herein, a simulation experiment is conducted with respect to a container ship, and the optimization route is compared and analyzed. Experimental results show that the proposed algorithm can plan a series of feasible ship routes to ensure safety, greenness, and economy and that it provides route selection references for captains and shipping companies.
Improvements in the Resistance of the Banana Species to Fusarium Wilt: A Systematic Review of Methods and Perspectives
Authors: Rocha Anelita de Jesus , Soares Julianna Matos da Silva , Nascimento Fernanda dos Santos , Santos Adriadna Souza , Amorim Vanusia Batista de Oliveira , Ferreira Claudia Fortes , Haddad Fernando , Santos-Serejo Janay Almeida dos , Amorim Edson Perito
Published: March 2021

The fungus Fusarium oxysporum f. sp. cubense (FOC), tropical race 4 (TR4), causes Fusarium wilt of banana, a pandemic that has threatened the cultivation and export trade of this fruit. This article presents the first systematic review of studies conducted in the last 10 years on the resistance of Musa spp. to Fusarium wilt. We evaluated articles deposited in different academic databases, using a standardized search string and predefined inclusion and exclusion criteria. We note that the information on the sequencing of the Musa sp. genome is certainly a source for obtaining resistant cultivars, mainly by evaluating the banana transcriptome data after infection with FOC. We also showed that there are sources of resistance to FOC race 1 (R1) and FOC TR4 in banana germplasms and that these data are the basis for obtaining resistant cultivars, although the published data are still scarce. In contrast, the transgenics approach has been adopted frequently. We propose harmonizing methods and protocols to facilitate the comparison of information obtained in different research centers and efforts based on global cooperation to cope with the disease. Thus, we offer here a contribution that may facilitate and direct research towards the production of banana resistant to FOC.
Depopulation and Extinction of Villages in Moravia and the Czech Part of Silesia since World War II
Authors: Vaishar Antonín , Vavrouchová Hana , Lešková Andrea , Peřinková Veronika
Published: March 2021

A thousand villages disappeared in Czechia during the course of historic development. There are two basic causes of the disappearance of villages: artificial human intervention (planned changes or hostile liquidation) and gradual depopulation due to remoteness and poor conditions for development, possibly in combination with natural disasters. The greatest number of extinct villages is related to the period after World War II, when many villages in the borderland, from which the German population was displaced, were demolished or abandoned. The aim of the article is to clarify the causes of the extinction of villages, its impact on the landscape and on the settlement system, as well as the possibilities of preserving the historical and cultural memory of extinct places. Finally, the danger of extinction of villages at the present time is discussed. This article points out that it is currently not necessary or effective to have the dense network of settlements that was present in the Middle Ages, when the population density was conditional to the limited technological potential of agriculture and transport. The main argument for preserving villages is their spiritual and cultural value, and their genius loci. However, this can be preserved without keeping the physical structure. At this time, the smallest settlements are changing from being permanently inhabited, to becoming second homes sites.
Breaking the Vicious Cycle between Illness and Poverty: Empirical Actions on Land Use in an Oasis Agricultural Area
Authors: Zhang Lu , Zhao Yannan
Published: March 2021

Illness and poverty have been identified to be mutually influential, thereby forming a vicious cycle. Cutting off this vicious circle will be of great significance in the long-term planning of rural poverty reduction. Most of the existing studies have been conducted in proposing medical policies. Thus, these policies neglect the ability of the poor themselves. In oasis agricultural areas, the land is the critical resource, and improving land-use efficiency has been proposed as the key entry point for breaking the vicious cycle of poverty and illness. This article summarizes the success achieved in fighting health-related poverty in South Xinjiang, China, which can be attributed to a three-pronged approach that addresses the construction of the living, production, and ecological spaces. (1) Construction of the living space should be the first step in breaking the vicious cycle of poverty and illness since it can improve the medical and basic living infrastructure. (2) Construction of the production space is critical for breaking the vicious cycle since it can directly increase the income of the poor. Specifically, the profit can be improved by increasing crop yields, developing animal husbandry, and participating in land circulation and rural cooperatives. Additionally, the construction of township enterprises can provide employment to the poor. (3) In regard to the construction of the ecological space, preventing and controlling desertification, as well as renovating the village environment, is essential for providing a good living environment that is conducive to the improvement of physical fitness. Constructing the multi-spaces of “living–production–ecological” has important implications in terms of transforming the vicious cycle into a virtuous one, which is crucial for designing national poverty reduction policies.
Establishing SW1353 Chondrocytes as a Cellular Model of Chondrolysis
Authors: Pang Kok-Lun , Chow Yoke Yue , Leong Lek Mun , Law Jia Xian , Ghafar Norzana Abd , Soelaiman Ima Nirwana , Chin Kok-Yong
Published: March 2021

Osteoarthritis (OA) is the most common degenerative joint disease characterised by chondrocyte cell death. An in vitro model of chondrocyte cell death may facilitate drug discovery in OA management. In this study, the cytotoxicity and mode of cell death of SW1353 chondrocytes treated with 24 h of OA inducers, including interleukin-1β (IL-1β), hydrogen peroxide (H2O2) and monosodium iodoacetate (MIA), were investigated. The microscopic features, oxidative (isoprostane) and inflammatory markers (tumour necrosis factor-alpha; TNF-α) for control and treated cells were compared. Our results showed that 24 h of H2O2 and MIA caused oxidative stress and a concentration-dependent reduction of SW1353 cell viability without TNF-α level upregulation. H2O2 primarily induced chondrocyte apoptosis with the detection of blebbing formation, cell shrinkage and cellular debris. MIA induced S-phase arrest on chondrocytes with a reduced number of attached cells but without significant cell death. On the other hand, 24 h of IL-1β did not affect the cell morphology and viability of SW1353 cells, with a significant increase in intracellular TNF-α levels without inducing oxidative stress. In conclusion, each OA inducer exerts differential effects on SW1353 chondrocyte cell fate. IL-1β is suitable in the inflammatory study but not for chondrocyte cell death. H2O2 and MIA are suitable for inducing chondrocyte cell death and growth arrest, respectively.
The Role of Consecutive Plasma Copeptin Levels in the Screening of Delayed Cerebral Ischemia in Poor-Grade Subarachnoid Hemorrhage
Authors: Rhim Jong Kook , Youn Dong Hyuk , Kim Bong Jun , Kim Youngmi , Kim Sungeun , Kim Heung Cheol , Jeon Jin Pyeong
Published: March 2021

The prognostic value of copeptin in subarachnoid hemorrhage (SAH) has been reported, but the prognosis was largely affected by the initial clinical severity. Thus, the previous studies are not very useful in predicting delayed cerebral ischemia (DCI) in poor-grade SAH patients. Here, we first investigated the feasibility of predicting DCI in poor-grade SAH based on consecutive measurements of plasma copeptin. We measured copeptin levels of 86 patients on days 1, 3, 5, 7, 9, 11, and 13 using ELISA. The primary outcome was the association between consecutive copeptin levels and DCI development. The secondary outcomes were comparison of copeptin with C-reactive protein (CRP) in predicting DCI. Additionally, we compared the prognostic value of transcranial Doppler ultrasonography (TCD) with copeptin using TCD alone to predict DCI. Increased copeptin (OR = 1.022, 95% CI: 1.008–1.037) and modified Fisher scale IV (OR = 2.841; 95% CI: 0.998–8.084) were closely related to DCI. Consecutive plasma copeptin measurements showed significant differences between DCI and non-DCI groups (p < 0.001). Higher CRP and DCI appeared to show a correlation, but it was not statistically significant. Analysis of copeptin changes with TCD appeared to predict DCI better than TCD alone with AUCROC differences of 0.072. Consecutive measurements of plasma copeptin levels facilitate the screening of DCI in poor-grade SAH patients.
Viscoelastic Polyurethane Foams for Use in Seals of Respiratory Protective Devices
Authors: Okrasa Małgorzata , Leszczyńska Milena , Sałasińska Kamila , Szczepkowski Leonard , Kozikowski Paweł , Majchrzycka Katarzyna , Ryszkowska Joanna
Published: March 2021

A key factor in effective protection against airborne hazards, i.e., biological and nonbiological aerosols, vapors, and gases, is a good face fit of respiratory protective devices (RPDs). Equally important is the comfort of use, which may encourage or discourage users from donning RPDs. The objective of the work was to develop viscoelastic polyurethane foams for use in RPD seals. The obtained foams were characterized using scanning electron microscopy, infrared spectroscopy, thermogravimetry, and differential scanning calorimetry. Measurements also involved gel fraction, apparent density, air permeability, elastic recovery time, compression set, rebound resilience, and sweat uptake. The results were discussed in the context of modifications to the foam formulation: the isocyanate index (INCO) in the range of 0.6–0.9 and the blowing agent content in the range of 1.2–3.0 php. FTIR analysis revealed a higher level of urea groups with increasing water content in the formulation. Higher INCO and water content levels also led to lower onset temperatures of thermal degradation and higher glass-transition temperatures of the soft phase. A decrease in apparent density and an increase in mean pore sizes of the foams with increasing INCO and water content levels was observed. Functional parameters (air permeability, elastic recovery time, compression set, rebound resilience, and sweat uptake) were also found to be satisfactory at lower INCO and water content levels.
Composite Binder Containing Industrial By-Products (FCCCw and PSw) and Nano SiO2
Authors: Banevičienė Vilma , Malaiškienė Jurgita , Zach Jiri , Dvorak Karel
Published: March 2021

This article analyzes the integrated effect of industrial by-products (spent fluidized bed catalytic cracking catalyst waste (FCCCw) and paper sludge waste (PSw) generated in paper manufacturing) combined with nano-SiO2 (NS) on the properties of cement binder, when a certain part of the binder is replaced with the said by-products in the cement mix. Standard testing methods were used to analyze the physical and mechanical properties of cement-based materials. For structure analysis, we used X-ray diffraction (XRD), derivative thermogravimetry (DTG), mercury intrusion porosimetry (MIP) and scanning electron microscopy (SEM). It was found that the replacement of cement by a combined additive of FCCCw, PSw and NS is important not only for ecological reasons (abatement of CO2 emissions and recovery of waste through secondary raw materials), but also in order to enhance the properties of cement-based binders. Presumably, higher amounts of calcium silicate hydrate (CSH) and calcium alumina silicate hydrate (CASH) in the compound binder are the result of the low content of portlandite and alite in the test specimens. The specimens modified with all three additives had the highest density (~2100 kg/m3), ultrasonic pulse velocity (UPV) (~4160 m/s) and compressive strength (~105 MPa), which was ~40% higher than in the control specimens. The average pore diameter of the complex binder decreased by 21%, whereas the median pore diameter decreased by 47%.
Characterization of the Compressive Load on a Lithium-Ion Battery for Electric Vehicle Application
Authors: Madani Seyed Saeed , Schaltz Erik , Kær Søren Knudsen
Published: March 2021

Lithium-ion batteries are being implemented in different large-scale applications, including aerospace and electric vehicles. For these utilizations, it is essential to improve battery cells with a great life cycle because a battery substitute is costly. For their implementation in real applications, lithium-ion battery cells undergo extension during the course of discharging and charging. To avoid disconnection among battery pack ingredients and deformity during cycling, compacting force is exerted to battery packs in electric vehicles. This research used a mechanical design feature that can address these issues. This investigation exhibits a comprehensive description of the experimental setup that can be used for battery testing under pressure to consider lithium-ion batteries’ safety, which could be employed in electrified transportation. Besides, this investigation strives to demonstrate how exterior force affects a lithium-ion battery cell’s performance and behavior corresponding to static exterior force by monitoring the applied pressure at the dissimilar state of charge. Electrochemical impedance spectroscopy was used as the primary technique for this research. It was concluded that the profiles of the achieved spectrums from the experiments seem entirely dissimilar in comparison with the cases without external pressure. By employing electrochemical impedance spectroscopy, it was noticed that the pure ohmic resistance, which is related to ion transport resistance of the separator, could substantially result in the corresponding resistance increase.
Nonlinearities and Chaos: A New Analysis of CEE Stock Markets
Authors: Albulescu Claudiu Tiberiu , Tiwari Aviral Kumar , Kyophilavong Phouphet
Published: March 2021

After a long transition period, the Central and Eastern European (CEE) capital markets have consolidated their place in the financial systems. However, little is known about the price behavior and efficiency of these markets. In this context, using a battery of tests for nonlinear and chaotic behavior, we look for the presence of nonlinearities and chaos in five CEE stock markets. We document, in general, the presence of nonlinearities and chaos which questions the efficient market hypothesis. However, if all tests highlight a chaotic behavior for the analyzed index returns, there are noteworthy differences between the analyzed stock markets underlined by nonlinearity tests, which question, thus, their level of significance. Moreover, the results of nonlinearity tests partially contrast the previous findings reported in the literature on the same group of stock markets, showing, thus, a change in their recent behavior, compared with the 1990s.
Neighbor Sum Distinguishing Total Choosability of IC-Planar Graphs without Theta Graphs Θ2,1,2
Authors: Zhang Donghan
Published: March 2021

A theta graph Θ2,1,2 is a graph obtained by joining two vertices by three internally disjoint paths of lengths 2, 1, and 2. A neighbor sum distinguishing (NSD) total coloring ϕ of G is a proper total coloring of G such that ∑z∈EG(u)∪{u}ϕ(z)≠∑z∈EG(v)∪{v}ϕ(z) for each edge uv∈E(G), where EG(u) denotes the set of edges incident with a vertex u. In 2015, Pilśniak and Woźniak introduced this coloring and conjectured that every graph with maximum degree Δ admits an NSD total (Δ+3)-coloring. In this paper, we show that the listing version of this conjecture holds for any IC-planar graph with maximum degree Δ≥9 but without theta graphs Θ2,1,2 by applying the Combinatorial Nullstellensatz, which improves the result of Song et al.
Correction: Gušić, D. Prime Geodesic Theorems for Compact Locally Symmetric Spaces of Real Rank One. Mathematics 2020, 8, 1762
Authors: Gušić Dženan
Published: March 2021

The author wishes to make the following correction to the paper [...]
Autopsy Tool in Unknown Diseases: The Experience with Coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2)
Authors: Sessa Francesco , Salerno Monica , Pomara Cristoforo
Published: March 2021

In the last two decades, three unknown pathogens have caused outbreaks, generating severe global health concerns. In 2003, after nucleic acid genotyping, a new virus was named severe acute respiratory syndrome coronavirus (SARS-CoV). After nine years, another coronavirus emerged in the middle east and was named MERS-CoV (Middle East Respiratory Syndrome—Coronavirus). Finally, in December 2019, a new unknown coronavirus was isolated from a cluster of patients and was named SARS-CoV-2 (COVID-19, coronavirus disease 2019). This review aims to propose a complete overview of autopsy in the three coronaviruses over the past two decades, showing its pivotal role in the management of unknown diseases. A total of 116 studies fulfilled the inclusion criteria: 14 studies were collected concerning SARS-CoV (87 autopsy reports, from Asian and American countries), 2 studies for MERS-CoV (2 autopsy reports, from Middle-East Asian countries), and 100 studies on SARS-CoV-2 (930 autopsy reports). Analyzing the data obtained on COVID-19, based on the country criterion, a large number of post-mortem investigation were performed in European countries (580 reports), followed by American countries (251 reports). It is interesting to note that no data were found from the Oceanic countries, maybe because of the minor involvement of the outbreak. In all cases, autopsy provided much information about each unknown coronavirus. Despite advanced technologies in the diagnostic fields, to date, autopsy remains the gold standard method to understand the biological features and the pathogenesis of unknown infections, especially when awareness of a pathogen is restricted and the impact on the healthcare system is substantial. The knowledge gained through this technique may positively influence therapeutic strategies, ultimately reducing mortality.
Ultrasound-Guided Regional Anesthesia–Current Strategies for Enhanced Recovery after Cardiac Surgery
Authors: Balan Cosmin , Bubenek-Turconi Serban-Ion , Tomescu Dana Rodica , Valeanu Liana
Published: March 2021

With the advent of fast-track pathways after cardiac surgery, there has been a renewed interest in regional anesthesia due to its opioid-sparing effect. This paradigm shift, looking to improve resource allocation efficiency and hasten postoperative extubation and mobilization, has been pursued by nearly every specialty area in surgery. Safety concerns regarding the use of classical neuraxial techniques in anticoagulated patients have tempered the application of regional anesthesia in cardiac surgery. Recently described ultrasound-guided thoracic wall blocks have emerged as valuable alternatives to epidurals and landmark-driven paravertebral and intercostal blocks. These novel procedures enable safe, effective, opioid-free pain control. Although experience within this field is still at an early stage, available evidence indicates that their use is poised to grow and may become integral to enhanced recovery pathways for cardiac surgery patients.
2,6-Bis((benzoyl-R)amino)pyridine (R = H, 4-Me, and 4-NMe2) Derivatives for the Removal of Cu(II), Ni(II), Co(II), and Zn(II) Ions from Aqueous Solutions in Classic Solvent Extraction and a Membrane Extraction
Authors: Bożejewicz Daria , Ośmiałowski Borys , Kaczorowska Małgorzata Anna , Witt Katarzyna
Published: March 2021

In this paper, the application of new substituted 2,6-bis((benzoyl-R)amino)pyridine (R = H, 4-Me, and 4-NMe2) derivatives for the recovery of copper(II), nickel(II), cobalt(II), and zinc(II) ions from aqueous solutions was described. The structures of the synthesized compounds were confirmed by nuclear magnetic resonance spectroscopy (NMR), electrospray ionization high-resolution mass spectrometry (ESI HRMS), and tandem mass spectrometry methods (HCD MS/MS). Three different derivatives of 2,6-bis((benzoyl-R)amino)pyridine were used as carriers in membrane processes and as extractants in classic solvent extraction. In each case, the single derivative recovery was carried out on a model solution that contained only one type of metal ions. Spectrophotometry studies were performed to determine the stability constants of the complexes formed by the synthesized species with analyzed metals ions. The results obtained indicate that the synthesized compounds form stable complexes with Cu(II), Ni(II), Co(II), and Zn(II) ions and can be used in both types of studied recovery processes. However, the effectiveness of the synthesized compounds in the recovery of metal ions depends both on the structure of compounds and properties of metals as well as on their concentration.
Oxytocin Downregulates the CaV1.2 L-Type Ca2+ Channel via Gi/cAMP/PKA/CREB Signaling Pathway in Cardiomyocytes
Authors: Morishima Masaki , Tahara Shintaro , Wang Yan , Ono Katsushige
Published: March 2021

Oxytocin (OT) and its receptor (OTR) are expressed in the heart and are involved in the physiological cardiovascular functional system. Although it is known that OT/OTR signaling is cardioprotective by reducing the inflammatory response and improving cardiovascular function, the role of OT in the cardiac electrical excitation modulation has not been clarified. This study investigates the molecular mechanism of the action of OT on cardiomyocyte membrane excitation focusing on the L-type Ca2+ channel. Our methodology uses molecular biological methods and a patch-clamp technique on rat cardiomyocytes with OT, combined with several signal inhibitors and/or activators. Our results show that long-term treatment of OT significantly decreases the expression of Cav1.2 mRNA, and reduces the L-type Ca2+ channel current (ICa.L) in cardiomyocytes. OT downregulates the phosphorylated component of a transcription factor adenosine-3′,5′-cyclic monophosphate (cAMP) response element binding protein (CREB), whose action is blocked by OTR antagonist and pertussis toxin, a specific inhibitor of the inhibitory GTP-binding regulators of adenylate cyclase, Gi. On the other hand, the upregulation of Cav1.2 mRNA expression by isoproterenol is halted by OT. Furthermore, inhibition of phospholipase C (PLC) was without effect on the OT action to downregulate Cav1.2 mRNA—which suggests a signal pathway of Gi/protein kinase A (PKA)/CREB mediated by OT/OTR. These findings indicate novel signaling pathways of OT contributing to a downregulation of the Cav1.2-L-type Ca2+ channel in cardiomyocytes.
Effect of Heat Treatment on the Microstructure and Mechanical Properties of Selective Laser-Melted Ti64 and Ti-5Al-5Mo-5V-1Cr-1Fe
Authors: Bai Hongjie , Deng Hao , Chen Longqing , Liu Xianbo , Qin Xiaorong , Zhang Dingguo , Liu Tong , Cui Xudong
Published: March 2021

Additive manufacturing (AM) has shown the ability in processing titanium alloys. However, due to the unique thermal history in AM, the microstructure of AM-fabricated parts is metastable and non-equilibrium. This work was aiming to tailor the microstructure and to improve the mechanical properties of α+β Ti-6Al-4V alloy and metastable β Ti-5Al-5Mo-5V-1Cr-1Fe alloys by manipulating the post-process heat treatment. The results showed that Ti-6Al-4V exhibited a metastable α’ martensite microstructure in the as-fabricated condition, while a metastable β structure was formed in as-printed Ti-5Al-5Mo-5V-1Cr-1Fe. After post-process heat treatment, both lamellar and bimodal microstructures were obtained in Ti64 and Ti-5Al-5Mo-5V-1Cr-1Fe alloys. Especially, the Ti-6Al-4V alloy subjected to 950 °C annealing showed the lamellar structure with the highest fracture toughness of 90.8 ± 2.1 MPa.m1/2. The one cyclically heat-treated has excellent combined strength, ductility and fracture toughness attributed to the bimodal structure. In addition, similar observations of lamellar and bimodal microstructure appeared in the post-process heat-treated Ti-5Al-5Mo-5V-1Cr-1Fe alloy. This study demonstrated that heat treatment is an effective way to tackle the non-equilibrium microstructure and improve the mechanical properties of selective laser melting (SLM)-fabricated titanium alloys.