bluuebunny/crossref_metadata_2025 huggingface dataset:
Neutrophils: tissue and circulating signatures of pediatric non-alcoholic fatty liver disease
Authors: Săsăran Maria Oana , Muntean Carmen , Lupu Ancuța , Lupu Vasile Valeriu
Published: January 2024

The recent rise in non-alcoholic fatty liver disease (NAFLD) among children and adolescents led to a thorough investigation of the peculiarities of the cellular infiltrate which characterize the disease at young ages. This review aims to highlight the key involvement of neutrophils in the pathogenesis of pediatric NAFLD and the potential biomarker role of neutrophil-to-lymphocyte ratio (NLR) in the same pediatric disorder. Neutrophils, which are first responders to inflammation, constitute an abundant component of an infiltrate which is particularly disposed within the portal area of children with NAFLD. The involvement of neutrophils in triggering liver fibrosis has been related amongst others to reactive oxygen species (ROS) production, to the stimulation of hepatic stellate cells, and to their synthesis of neutrophil elastase. As immune imbalance characterizes NAFLD, potentially emerging non-invasive biomarkers such as NLR have been proposed for the detection and prognosis of NAFLD. In adults, several studies asserted the role of NLR in the prediction of advancing liver fibrosis and mortality in subjects with NAFLD. In children, data is scarce with contradicting findings, as some studies failed to identify significant shifting in NLR values in children with NAFLD when compared with obese controls without liver impairment. However, NLR seems to significantly increase in children with obesity and different degrees of NAFLD when compared to healthy counterparts and their changes seem to be reversible with weight loss. Still, paucity of pediatric studies calls for future research addressing the role of NLR in predicting NAFLD development and progression in children with obesity.
Aspergillus terreus spondylodiscitis following acupuncture and acupotomy in an immunocompetent host: case report and literature review
Authors: Jin Yufei , Yin Xiang
Published: January 2024

Aspergillus terreus is a fungus responsible for various infections in human beings; however, spine involvement is uncommon. Herein, we report a case of A. terreus spondylodiscitis following acupuncture and acupotomy in an immunocompetent Chinese patient. Admission lumbar magnetic resonance imaging (MRI) revealed infection at the L4/5 level without significant vertebral destruction. After unsuccessful symptomatic and anti-tuberculosis treatments, A. terreus was identified through culture, microscopy of isolate, histological examination and VITEK system. Intravenous voriconazole was then given; however, the patient’s spinal condition deteriorated rapidly, resulting in evident destruction of the L4/5 vertebral bodies. Surgeries including L4/5 intervertebral disc debridement, spinal canal decompression, posterior lumbar interbody fusion (PLIF) with allogeneic fibula ring fusion cages, and posterior pedicle screw fixation were then performed. Imaging findings at one-month and six-month follow-up suggested that the patient was successfully treated. This case highlighted two important points: firstly, although acupuncture and acupotomy are generally regarded as safe conservative treatments for pain management, they can still lead to complications such as fungal spinal infection. Therefore, vigilance is necessary when considering these treatments; secondly, PLIF with allogeneic fibula ring fusion cages may be beneficial for A. terreus spondylodiscitis patients with spinal instability.
Association of sex with post-arrest care and outcomes after out-of-hospital cardiac arrest of initial shockable rhythm: a nationwide cohort study
Authors: Hosomi Sanae , Irisawa Taro , Nakao Shunichiro , Zha Ling , Kiyohara Kousuke , Kitamura Tetsuhisa , Ogura Hiroshi , Oda Jun
Published: January 2024

Background

Research has described differences in the provision of prehospital treatment for women who experience out-of-hospital cardiac arrest. However, studies have reported conflicting results regarding survival outcomes or in-hospital interventions between sexes. Thus, this study aimed to investigate the association of sex with survival outcomes and in-hospital treatments in Japan.

Methods

We retrospectively analyzed data from the Japanese Association for Acute Medicine–Out-of-Hospital Cardiac Arrest Registry. Patients aged ≥18 years who presented with a shockable rhythm at the scene between June 2014 and December 2020 were included in our analysis. Outcome measures were 30-day survival and in-hospital interventions. We compared the outcomes between the sexes using multivariable logistic regression.

Results

In total, 5,926 patients (4,270 men; 1,026 women) with out-of-hospital cardiac arrest were eligible for our analysis. The proportions of patients with 30-day survival outcomes were 39.5% (1685/4,270) and 37.4% (384/1,026) in the male and female groups, respectively (crude odds ratio, 0.92; 95% confidence interval, 0.80–1.06). Although there were no significant differences, survival outcomes tended to be better in women than in men in the multiple regression analysis (adjusted odds ratio: 1.38; 95% confidence interval: 0.82–2.33). Furthermore, there was no significant difference between the sexes in terms of patients who received extracorporeal cardiopulmonary resuscitation (adjusted odds ratio: 0.81; 95% confidence interval: 0.49–1.33) or targeted temperature management (adjusted odds ratio: 0.99; 95% confidence interval: 0.68–1.46).

Conclusions

After adjusting for prognostic factors, there were no differences in survival rates and in-hospital interventions between men and women.
Local impedance and contact force guidance to predict successful cavotricuspid isthmus ablation with a zero-fluoroscopy approach
Authors: Melero-Polo Jorge , Cabrera-Ramos Mercedes , Alfonso-Almazán Jose Manuel , Marín-García Isabel , Montilla-Padilla Isabel , Ruiz-Arroyo José Ramón , López-Rodríguez Guillermo , Ramos-Maqueda Javier
Published: January 2024

Introduction

A new technology capable of monitoring local impedance (LI) and contact force (CF) has recently been developed. At the same time, there is growing concern regarding catheter ablation performed under fluoroscopy guidance, due to its harmful effects for both patients and practitioners. The aim of this study was to assess the safety and effectiveness of zero-fluoroscopy cavotricuspid isthmus (CTI) ablation monitoring LI drop and CF as well as to elucidate if these parameters can predict successful radiofrequency (RF) applications in CTI ablation.

Methods

We conducted a prospective observational study recruiting 50 consecutive patients who underwent CTI ablation. A zero-fluoroscopy approach guided by the combination of LI drop and CF was performed. In each RF application, CF and LI drop were monitored. A 6-month follow-up visit was scheduled to assess recurrences.

Results

A total of 767 first-pass RF applications were evaluated in 50 patients. First-pass effective RF applications were associated with greater LI drops: absolute LI drops (30.05 ± 6.23 Ω vs. 25.01 ± 5.95 Ω), p = 0.004) and relative LI drops (−23.3 ± 4.9% vs. −18.3 ± 5.6%, p = 0.0005). RF applications with a CF between 5 and 15 grams achieved a higher LI drop compared to those with a CF below 5 grams (29.4 ± 8.76 Ω vs. 24.8 ± 8.18 Ω, p < 0.0003). However, there were no significant differences in LI drop between RF applications with a CF between 5 and 15 grams and those with a CF beyond 15 grams (29.4 ± 8.76 Ω vs. 31.2 ± 9.81 Ω, p = 0.19). CF by itself, without considering LI drop, did not predict effective RF applications (12.3 ± 7.54 g vs. 11.18 ± 5.18 g, p = 0.545). Successful CTI ablation guided by a zero-fluoroscopy approach was achieved in all patients. Only one patient experienced a recurrence during the 6-month follow-up.

Conclusions

LI drop (absolute and relative values) appears to be a good predictor of successful RF applications to achieve CTI conduction block. The optimal CF to achieve a good LI drop is between 5 and 15 g. A zero-fluoroscopy approach guided by LI and CF was feasible, effective, and safe.
The autonomic nervous system in atrial fibrillation—pathophysiology and non-invasive assessment
Authors: Vandenberk Bert , Haemers Peter , Morillo Carlos
Published: January 2024

The autonomic nervous system plays a crucial role in atrial fibrillation pathophysiology. Parasympathetic hyperactivity result in a shortening of the action potential duration, a reduction of the conduction wavelength, and as such facilitates reentry in the presence of triggers. Further, autonomic remodeling of atrial myocytes in AF includes progressive sympathetic hyperinnervation by increased atrial sympathetic nerve density and sympathetic atrial nerve sprouting. Knowledge on the pathophysiological process in AF, including the contribution of the autonomic nervous system, may in the near future guide personalized AF management. This review focuses on the role of the autonomic nervous system in atrial fibrillation pathophysiology and non-invasive assessment of the autonomic nervous system.
Age at first birth, age at menopause, and risk of ovarian cyst: a two-sample Mendelian randomization study
Authors: Su Qian , Yang Zhiyong
Published: January 2024

Background

Increasing observational studies have indicated that hormonal reproductive factors were associated with ovarian cyst, a common gynecological disease. A two-sample Mendelian randomization (MR) was carried out by investigating the causality of reproductive factors including age at first birth (AFB), age at natural menopause (ANM), and age at menarche (AAM), and the risk of ovarian cyst (OC).

Method

Summary statistics were collected from a large genome-wide association study (GWAS), and we used a two-sample MR study to clarify the causal association between the exposure of AFB (N = 542,901), ANM (N = 69,360), and AAM (N = 29,346) and the outcome of the OC (Ncase = 20,750, Ncontrol = 107,564). We separately selected 51, 35, and 6 single-nucleotide polymorphisms (SNPs) as instrumental variables (IVs) for assaying the influence of AFB, ANM, and AAM on OC, respectively. Then, the causal relationship was tested through multiple approaches including an inverse-variance weighted method, an MR-Egger regression, and a weighted median method. In addition, the MR-PRESSO method was also used to verify the horizontal pleiotropy. Subsequently, we adjust the confounders for MR design.

Results

The MR analysis results showed that AFB was negatively associated with the OC (IVW Beta: −0.09, OR: 0.91, 95% CI: 0.86–0.96, p = 0.00185), and the greater AAM decreased the risk of OC (IVW Beta: −0.10, OR: 0.91, 95% CI: 0.82–0.99, p = 0.0376). However, ANM has a positive correlation with the OC (IVW Beta: 0.05, OR: 1.05, 95% CI: 1.03–1.08, p = 8.38 × 10−6). After adjusting BMI, alcohol intake frequency, and ever smoked, we also obtained a negative relationship between AFB and OC (p < 0.005). Meanwhile, we adjusted weight, alcohol intake frequency, and height, and then found a causal relationship between older AMN and an increased risk of OC (p < 0.005).

Conclusion

A causal effect of reproductive factors on the development of OC, affected by AFB, ANM, and AAM, was found convincingly. After adjusting the confounders, we also successfully found the substantial causal effect of younger AFB, younger AAM, and older ANM on an increased risk of OC.
Correlation between the non-use of cooking oil fume extractors and bone mineral density in population aged 45 years and older in China: a cross-sectional study
Authors: Zhang Haitao , Shi Binhao , Yuan Chunchun , Huang Chen , Huang Tingrui , Liao Zhangyu , Zhu Wenhao , Zhong Wei , Xu Hongbin , Ji Jiangxun , Cai Feihong , Chen Yue , Sun Pan , Zeng Xianhui , Yang Zhiwu , Wang Jing , Shu Bing , Liang Qianqian , Shi Qi , Xu Chuanglong , Tang Dezhi , Wang Yongjun
Published: January 2024

Introduction

The correlation between the non-use of cooking oil fumes (COFs) extractors and bone mineral density (BMD) have not been clarified. Consequently, this study attempted to explore the impact of non-use COFs extractors on BMD in population aged 45 years and older based on a cross-sectional study.

Methods

This study was a cross-sectional study within the framework of an ongoing prospective population-based cohort study in China. The multivariate linear regression models were used to evaluate the correlation between the non-use of fume extractors in family cooking and total lumbar spine (LS), femoral neck (FN), total hip BMD and levels of bone metabolism markers.

Results

A total of 3433 participants were included in the final analyses, of which 2607 (75.93%) participants used fume extractors. The results of models indicated that there were significant correlations of the non-use of fume extractors on total LS BMD (β = -0.024, 95% CI, -0.036, -0.012, p < 0.001), PINP (β = 4.363, 95% CI, 2.371, 6.356, p < 0.001) and ALP (β = 4.555, 95% CI, 2.593, 6.517, p < 0.001) levels.

Conclusions

This study verified that the use of fume extractors is an efficacious measure to prevent LS bone loss. For the sake of public bone health, people should install a fume extractor in the kitchen and use it routinely when cooking.
Could setmelanotide be the game-changer for acquired hypothalamic obesity?
Authors: van Santen Hanneke M. , Denzer Christian , Müller Hermann Lothar
Published: January 2024

Children with acquired hypothalamic obesity, e.g. following treatment for pediatric craniopharyngioma are at great risk for metabolic syndrome, cardiovascular health problems and premature mortality. Treatment for acquired hypothalamic obesity has thus far been disappointing. Several interventions were reported to be partially successful, including dextro-amphetamine and GLP-1R agonists, although results in acquired hypothalamic obesity are conflicting. Disruption of signaling through the melanocortin-4 receptor (MC4R) pathway results in hyperphagia and severe early-onset hypothalamic obesity. Recently, the MC4R agonist setmelanotide has shown promising results in children with genetic forms of hypothalamic obesity; POMC, PCSK1 and LEPR. Patient quotes such as “we have our family life back” illustrate the magnitude of the effect. Targeted hormone replacement therapy with a MC4R agonist for acquired hypothalamic obesity could be a game-changer. Preliminary results of setmelanotide treatment in 14, mostly pediatric, patients with acquired hypothalamic obesity are promising. The FDA has recommended that a prospective, randomized, blinded trial be conducted over a 12 months treatment period, comparable to pivotal trials for other obesity drugs. It may be discussed whether setmelanotide should be regarded as an obesity drug or whether it may be envisioned as an agent for hypothalamic substitution therapy. In this commentary we discuss the trial that is currently recruiting patients with acquired hypothalamic obesity.
Analysis of coolant flow distribution characteristics at core inlet of small pressurized water reactor under rolling condition
Authors: Zeng Jinyuan , Mao Yulong , Li Bingwen , Zhu Jianmin , Hu Yisong , Chen Jun
Published: January 2024

The flow distribution characteristics at core inlet directly determines the thermal and hydraulic characteristics in the core, and it is closely related to the safety of nuclear reactor. The primary coolant is subjected to additional inertial force in ocean motion, which influence the flow distribution characteristics at core inlet. In the present paper, numerical simulation method is adopted to analyze the coolant flow distribution characteristics at core inlet of the Reactor Pressure Vessel (RPV) of a Small Reactor under rolling condition. Then the results of the flow distribution characteristics were compared with those under steady state. The results show the variation range of flow distribution factors in each channel under rolling condition is within 5%. And the uneven distribution of coolant at the core inlet increases with the increase of rolling amplitude, and decrease with the rolling period in short period. Standard deviation of flow distribution factor at the core inlet changes little under rolling condition, which indicates the flow distribution at the core inlet keeps good uniformity.
Has GVC participation exacerbated inequality of the embodied carbon in fossil energy industry export trade?
Authors: Zheng Minggui , Ni Jingsheng , Wen Donghong
Published: January 2024

Introduction: The fossil energy sector is an important part of international trade, and the flow of products between sectors will bring about the flow of embodied carbon. There are relatively few studies on embodied carbon in the fossil energy sector, which are mostly concentrated in other sectors and are not well integrated with GVC. Combining embodied carbon with GVC provides a better view of the allocation of environmental responsibility in trade. At the same time, it can also help different countries to improve the environment with the help of GVC.

Methods: This research is based on the World Input–Output Database. First, by using multi-regional input–output, this paper measures fossil energy trade embodied carbon (FEE) data of 39 countries from 2000 to 2014; second, using structural decomposition analysis, we find what factors affect FEE. Finally, we analyze theimpact of global value chain (GVC) on the FEE.

Results: This study obtained the following conclusions: 1) different GVCs have different effects on the embodied carbon in trade. Simple forward, complex forward, and simple backward to embedded play a positive role. The complex backward GVC has a negative impact; 2) high-income countries can get greater positive environmental effects through GVC, and middle-income countries cannot obtain or obtain less environmental effects; and 3) the backward GVC affects the FEE by affecting the proportion of fossil energy consumption.

Discussion: The results show that the FEE shall be jointly responsible for the world’s countries, and different countries should make different contributions to the world’s environmental protection comprehensively considering factors such as the economic development level of various countries. This proposes relevant policy suggestions in China.
Genetic and biomarker modulation of arterial stiffness change in the SardiNIA population cohort
Authors: Asefa Nigus G. , Meirelles Osorio , Lakatta Edward , Fiorillo Edoardo , Scuteri Angelo , Cucca Francesco , Marongiu Michele , Delitala Alessandro , Schlessinger David , Launer Lenore J.
Published: January 2024

Background and aims

Arterial stiffness (AS), quantified by pulse wave velocity (PWV), arises due to impaired arterial elastic tissue and smooth muscle dysfunction. We aimed to examine the longitudinal association of genetic, lipid and inflammation biomarkers with PWV and how these associations may change with aging.

Materials and methods

We utilized genotype and four time-point biomarker data from the SardiNIA cohort [n = 6,301; mean baseline age 43.3 (SD 17.3); 58% females]. To investigate the association of PWV with genetic variants, lipid, and inflammation biomarkers, we employed linear mixed modeling, using age as the time scale. Biomarkers exhibiting significant longitudinal associations were categorized into tertiles and individuals within the second tertile or those with heterozygous alleles were excluded, leaving a cohort of 2,000 individuals. This cohort was further divided into four risk groups: low genetic and low biomarker (L-L), low genetic and high biomarker (L-H), high genetic and low biomarker (H-L), and high genetic and high biomarker risk (H-H). Subsequent analyses focused on these risk groups to assess their association to PWV with time.

Results

Using the complete dataset, we found a significant longitudinal association of total cholesterol (TC), triglycerides (TG), fibrinogen (FGN), and total white blood cell count (TWBC) with PWV, all with p < 3.33 × 10−3. After grouping, individuals with homogeneous risk alleles of SNP rs3742207 and high baseline TG levels (H-H group) exhibited a 1.39-fold higher PWV (m/s) (95% CI, 1.17–1.64, p = 1.21 × 10−4) compared to the L-L group. Similarly, individuals in the H-H group of rs3742207-TWBC combination showed 1.75 times higher PWV (95% CI, 1.48–0.2.07, p = 1.01 × 10−10) compared to the L-L group. Similar patterns were observed for groups based on SNP rs7152623-TWBC risk. Furthermore, these associations became more pronounced with increasing age (p < 3.33 × 10−3).

Conclusion

The longitudinal association of TG and TWBC biomarkers with PWV varied by SNPs rs3742207 and rs7152623 genotype. Further studies are warranted to investigate the function of genetics, lipids, and inflammation biomarkers on PWV change.
A pan-cancer analysis of the prognostic implication and oncogenic role of tubulin epsilon and delta complex 2 (TEDC2) in human tumors
Authors: Liu Yang , Zhu Jie , Shen Jing , Lu Yuting , Pan Ke , Tong Chuan , Wang Yao
Published: January 2024

Introduction

Tubulin epsilon and delta complex 2 (TEDC2) is widely expressed in various human tissues and primarily governs centriole stability. However, the biological significance of TEDC2 in pan-cancer is unclear.

Methods

In this study, we employed R software and various online bioinformatics analysis tools to investigate the functional attributes of TEDC2 in human tumours and its potential involvement in immune response. The status of TEDC2 expression was evaluated in samples from the TCGA and GEO datasets, as well as in tumour and corresponding normal samples from the TCGA database. Subsequently, Kaplan-Meier estimates, clinical correlations, and univariate Cox regressions were used to analyze the 33 types of tumors from TCGA and determine the prognostic significance of TEDC2. Moreover, nomogram models were formulated using three distinct tumours, namely kidney renal clear cell carcinoma (KIRC), lung adenocarcinoma (LUAD), and liver hepatocellular carcinoma (LIHC), to evaluate the prognostic significance of TEDC2 in tumours. Furthermore, TEDC2 was investigated for its correlation with the levels of immune cell infiltration, and a functional enrichment analysis was conducted to identify potential signalling pathways involving TEDC2.

Results

Differential analysis revealed that 16 tumour types expressed TEDC2 to a greater extent than normal tissues. The abnormal expression of TEDC2 can predict survival outcomes in patients with adrenocortical carcinoma (ACC), KIRC, kidney renal papillary cell carcinoma (KIRP), LUAD, LIHC, lower grade glioma (LGG), and thymoma (THYM). Subsequent results indicated that TEDC2 has the ability to influence ECM regulators, cell cycle, and Immune checkpoint-associated signalling pathways, which could potentially lead to a poor prognosis and tumour progression.

Discussion

TEDC2 has been identified as a potential therapeutic target that could predict the prognosis of multiple tumour types, making it a promising target for reversing tumour development.
Peptoniphilus gorbachii alleviates collagen-induced arthritis in mice by improving intestinal homeostasis and immune regulation
Authors: Kim Suhee , Chun Sung Hak , Cheon Yun-Hong , Kim Mingyo , Kim Hyun-Ok , Lee Hanna , Hong Seong-Tshool , Park Sang-Jun , Park Myeong Soo , Suh Young Sun , Lee Sang-Il
Published: January 2024

Introduction

The intricate connection between gut microbiota and rheumatoid arthritis (RA) pathogenesis has gained prominence, although the specific microbial species contributing to RA development remain largely unknown. Recent studies have sought to comprehensively explore alterations in the human microbiome, focusing on identifying disease-related microbial species through blood analysis. Consequently, this study aimed to identify RA-associated microbial species using a serum microbial array system and to investigate the efficacy and underlying mechanisms of potential microbial species for RA treatment.

Methods

Serum immunoglobulin M levels against 384 intestinal microbial species were assessed using a microbial microarray in patients with RA and healthy individuals. We investigated the therapeutic potential of the identified microbial candidate regarding arthritis development, immune responses, gut barrier function, and gut microbiome using a collagen-induced arthritis (CIA) mouse model.

Results

Our findings revealed significant alterations in antibody levels against 36 microbial species in patients with RA compared to healthy individuals. Notably, the antibody levels against Peptoniphilus gorbachii (PG) were decreased in patients with RA and exhibited an inverse correlation with RA disease activity. In vitro experiments demonstrated that PG produced acetate and butyrate, while exhibiting anti-inflammatory properties. In CIA mice, PG administration suppressed arthritis symptoms, reduced the accumulation of inflammatory monocytes in the mesenteric lymph nodes, and downregulated gene expression of pro-inflammatory cytokines in the ileum. Additionally, PG supplementation restored intestinal barrier integrity and partially resolved gut microbial dysbiosis in CIA mice. The fecal microbiota in PG-treated mice corresponded to improved intestinal barrier integrity and reduced inflammatory responses.

Conclusion

This study highlights the potential of serum-based detection of anti-microbial antibodies to identify microbial targets at the species level for RA treatment. Moreover, our findings suggest that PG, identified through the microbial microarray analysis, holds therapeutic potential for RA by restoring intestinal barrier integrity and suppressing the immunologic response associated with RA.
Dendritic cells: the yin and yang in disease progression
Authors: Jiménez-Cortegana Carlos , Palomares Francisca , Alba Gonzalo , Santa-María Consuelo , de la Cruz-Merino Luis , Sánchez-Margalet Victor , López-Enríquez Soledad
Published: January 2024

Dendritic cells (DCs) are antigen presenting cells that link innate and adaptive immunity. DCs have been historically considered as the most effective and potent cell population to capture, process and present antigens to activate naïve T cells and originate favorable immune responses in many diseases, such as cancer. However, in the last decades, it has been observed that DCs not only promote beneficial responses, but also drive the initiation and progression of some pathologies, including inflammatory bowel disease (IBD). In line with those notions, different therapeutic approaches have been tested to enhance or impair the concentration and role of the different DC subsets. The blockade of inhibitory pathways to promote DCs or DC-based vaccines have been successfully assessed in cancer, whereas the targeting of DCs to inhibit their functionality has proved to be favorable in IBD. In this review, we (a) described the general role of DCs, (b) explained the DC subsets and their role in immunogenicity, (c) analyzed the role of DCs in cancer and therapeutic approaches to promote immunogenic DCs and (d) analyzed the role of DCs in IBD and therapeutic approaches to reduced DC-induced inflammation. Therefore, we aimed to highlight the “yin-yang” role of DCs to improve the understand of this type of cells in disease progression.
Longitudinal patterns of inflammatory mediators after acute HIV infection correlate to intact and total reservoir
Authors: De Clercq Jozefien , De Scheerder Marie-Angélique , Mortier Virginie , Verhofstede Chris , Vandecasteele Stefaan J. , Allard Sabine D. , Necsoi Coca , De Wit Stéphane , Gerlo Sarah , Vandekerckhove Linos
Published: January 2024

Background

Despite the beneficial effects of antiretroviral therapy (ART) initiation during acute HIV infection (AHI), residual immune activation remains a hallmark of treated HIV infection.

Methods

Plasma concentrations of 40 mediators were measured longitudinally in 39 early treated participants of a Belgian AHI cohort (HIV+) and in 21 HIV-negative controls (HIV-). We investigated the association of the inflammatory profile with clinical presentation, plasma viral load, immunological parameters, and in-depth characterization of the HIV reservoir.

Results

While levels of most soluble mediators normalized with suppressive ART, we demonstrated the persistence of a pro-inflammatory signature in early treated HIV+ participants in comparison to HIV- controls. Examination of these mediators demonstrated a correlation with their levels during AHI, which seemed to be viremia-driven, and suggested involvement of an activated myeloid compartment, IFN-γ-signaling, and inflammasome-related pathways. Interestingly, some of these pro-inflammatory mediators correlated with a larger reservoir size and slower reservoir decay. In contrast, we also identified soluble mediators which were associated with favorable effects on immunovirological outcomes and reservoir, both during and after AHI.

Conclusion

These data highlight how the persistent pro-inflammatory profile observed in early ART treated individuals is shaped during AHI and is intertwined with viral dynamics
Metabolomics-transcriptomics joint analysis: unveiling the dysregulated cell death network and developing a diagnostic model for high-grade neuroblastoma
Authors: Zhang Wancun , Zhang Mengxin , Sun Meng , Hu Minghui , Yu Muchun , Sun Jushan , Zhang Xianwei , Du Bang
Published: January 2024

High-grade neuroblastoma (HG-NB) exhibits a significantly diminished survival rate in comparison to low-grade neuroblastoma (LG-NB), primarily attributed to the mechanism of HG-NB is unclear and the lacking effective therapeutic targets and diagnostic model. Therefore, the current investigation aims to study the dysregulated network between HG-NB and LG-NB based on transcriptomics and metabolomics joint analysis. Meanwhile, a risk diagnostic model to distinguish HG-NB and LG-NB was also developed. Metabolomics analysis was conducted using plasma samples obtained from 48 HG-NB patients and 36 LG-NB patients. A total of 39 metabolites exhibited alterations, with 20 showing an increase and 19 displaying a decrease in HG-NB. Additionally, transcriptomics analysis was performed on NB tissue samples collected from 31 HG-NB patients and 20 LG-NB patients. Results showed that a significant alteration was observed in a total of 1,199 mRNAs in HG-NB, among which 893 were upregulated while the remaining 306 were downregulated. In particular, the joint analysis of both omics data revealed three aberrant pathways, namely the cAMP signaling pathway, PI3K-Akt signaling pathway, and TNF signaling pathway, which were found to be associated with cell death. Notably, a diagnostic model for HG-NB risk classification was developed based on the genes MGST1, SERPINE1, and ERBB3 with an area under the receiver operating characteristic curve of 0.915. In the validation set, the sensitivity and specificity were determined to be 75.0% and 80.0%, respectively.
Coastal environmental changes after the Saemangeum seawall construction
Authors: Baek Ji-Yeon , Guerreiro Catarina V. , Kim Jaeseong , Nam Jungho , Jo Young-Heon
Published: January 2024

The coastal environment in the Saemangeum area has experienced persistent physical stresses owing to the irregular operation of the sluice gates and related artificial disturbances since seawall construction, which has led to restricted freshwater-seawater circulation. To understand the impacts of stress, we performed long-term (1999-2022, 24 years) in situ measurements of relevant biotic and abiotic parameters and employed the random forest (RF) technique to determine the phytoplankton community response to environmental disturbance. Specifically, we estimated chlorophyll-a (Chl-a) concentrations using an RF model based on various environmental factors such as sea surface temperature (SST), sea surface salinity (SSS), dissolved oxygen saturation (DO), dissolved inorganic nitrogen (DIN), and dissolved inorganic phosphorus (DIP) as input variables. From the RF analysis, each environmental factor contributed to variation in Chl-a concentration as follows: SSS (42.91%), SST (17.88%), DIP (14.38%), DIN (13.36%), and DO (11.48%). In addition, we performed sensitivity experiments by altering the salinity, which was revealed to be the most influential environmental parameter. As a result, Chl-a concentration increased by approximately 1.79 times in lower salinity conditions (from 7 to 27 psu) compared to the normal salinity conditions prior to the seawall construction (from 12 to 32 psu) in both areas, including the inside and outside the seawall. More importantly, lower salinity conditions stimulated dinoflagellate blooms, that is, red tides, implying that restricted freshwater-seawater circulation could worsen coastal ecosystems. Thus, this study contributes to understanding the impacts of environmental changes caused by sluice gate manipulation on marine ecosystems, such as phytoplankton community dynamics. Moreover, this study recommends an ecologically suitable operation scheme for Saemangeum sluice gates to ensure a healthy coastal ecosystem.
Capture of zooplankton by site-attached fish: striking dynamics under different flow speeds and prey paths
Authors: Ella Hadar , Genin Amatzia
Published: January 2024

Consumption of pelagic zooplankton plays a vital role in the functioning of benthic communities such as coral reefs and kelp forests. Many fish that consume zooplankton in those habitats are site attached, foraging for drifting prey while maintaining a fixed position close to a shelter such as a branching coral or a perforated rock. Therefore, the flow, in which their planktonic prey drifts, is expected to affect their foraging movements. However, most attributes of those movements are poorly understood- a gap that our study seeks to fulfil. Our experiments were carried out in a laboratory flume with 4 common coral-reef site-attached species. Their movements were recorded in 3D, using two orthogonal video cameras. Different fishes exhibited similar trends despite noticeable differences in their body size, their morphology, the type of shelters they use, and the typical size of the groups in which they reside. In all species, the strike distance decreased with increasing flow speed. Similarly, the distance between the fish and prey at the moment of strike initiation (“Reactive Distance”) decreased with increasing flow speed, as well as the angle between that “Reactive Distance” and flow direction. Surprisingly, striking speeds (relative to Earth) remained nearly unchanged under different flows speeds. However, faster strikes occurred when oriented at wider angles relative to the flow. Taken together, the fish appear to determine the speed and angle of their strikes based on a cognitive ability to assess the prey’s drifting speed and path in order to reach on time the intercepting point. A rough estimate of the time it takes the fish to decide on the strike’s orientation and speed, would suggest a few hundred of milliseconds. Using published data on the fishes’ feeding rates, we found that the fish significantly differed in their feeding efficiencies, defined as the percent of prey they captured from those passing through their actual foraging space. That difference may explain inter-specific differences in the habitats the fish use and their group size.
Assessment of annual variability in the population status and reproductive cycle of purple sea urchins (Heliocidaris crassispina, Agassiz, 1864) in Daya Bay, China
Authors: Zhao Xinye , Mu Xiaohui , Guo Yu , Li Jiayang , Ma Zhenhua , Yu Gang , Qin Chuanxin
Published: January 2024

The purple sea urchin (Heliocidaris crassispina) is one of the main drivers of ecosystem dynamics, and its reproductive cycle plays an important role in population structure and size variability. In this study, we analyzed the population structure, gonadal developmental characteristics, reproductive cycle and other factors influencing wild purple sea urchins from December 2021 to November 2022 in Daya Bay, Guangdong, China. The results showed that purple sea urchins showed an allometric growth pattern, there were obvious seasonal variations in the population size, and fishing pressure reduced the sea urchin populations; two-factor analysis of variance (ANOVA) and correlation analyses showed negative correlations between month (M), test diameter (TD), weight (W) and gonadal index (GI) (P> 0.01), and a positive correlation between TD, weight (W), and GI (P<0.01); the gonadal development process can be divided into recovery, growth, prematuration, maturation, and late discharge, with almost synchronous development of male and female gametes; the spawning period is from April to November, which is longer than in the temperate zones, showing two spawning peaks in April and August. The time of spawning is influenced by temperature and food. Data on the breeding cycle of sea urchins in Daya Bay were obtained in this study. This information can offer theoretical assistance in the preservation and management of both artificial nurseries and wild resources.
Fecal microbiota transplantation repairs intestinal permeability and regulates the expression of 5-HT to influence alcohol-induced depression-like behaviors in C57BL/6J mice
Authors: Li Dezhi , Liang Wei , Zhang Wentong , Huang Zhiqiang , Liang Haipeng , Liu Qing
Published: January 2024

The epidemic of alcohol abuse affects millions of people worldwide. Relevant evidence supports the notion that the gut microbiota (GM) plays a crucial role in central nervous system (CNS) function, and its composition undergoes changes following alcohol consumption. Therefore, the purpose of this study was to investigate the effect of reconstructing the gut microbiota by fecal microbiota transplantation (FMT) on alcohol dependence. Here, we established an alcohol dependence model with C57BL/6J mice and proved that FMT treatment improved anxiety-like behavior and alcohol-seeking behavior in alcohol-dependent mice. Additionally, we found that the expression of the intestinal intercellular tight junction structure proteins ZO-1 and occludin was significantly increased after FMT. FMT repaired intestinal permeability in alcohol-dependent mice and decreased the levels of lipopolysaccharide (LPS) and proinflammatory factors. Moreover, the serotonin (5-hydroxytryptamine, 5-HT) content was significantly increased in alcohol-dependent mouse intestinal and brain tissues after receiving the fecal microbiome from healthy mice. 16S rRNA sequencing demonstrated that FMT markedly reshaped the composition of the gut microbiota and elicited changes in the intestinal barrier and 5-HT levels. Collectively, our results revealed that FMT has a palliative effect on alcohol dependence and explored the underlying mechanisms, which provides new strategies for the treatment of alcohol dependence.
Phylogenomics studies and molecular markers reliably demarcate genus Pseudomonas sensu stricto and twelve other Pseudomonadaceae species clades representing novel and emended genera
Authors: Rudra Bashudev , Gupta Radhey S.
Published: January 2024

Genus Pseudomonas is a large assemblage of diverse microorganisms, not sharing a common evolutionary history. To clarify their evolutionary relationships and classification, we have conducted comprehensive phylogenomic and comparative analyses on 388 Pseudomonadaceae genomes. In phylogenomic trees, Pseudomonas species formed 12 main clusters, apart from the “Aeruginosa clade” containing its type species, P. aeruginosa. In parallel, our detailed analyses on protein sequences from Pseudomonadaceae genomes have identified 98 novel conserved signature indels (CSIs), which are uniquely shared by the species from different observed clades/groups. Six CSIs, which are exclusively shared by species from the “Aeruginosa clade,” provide reliable demarcation of this clade corresponding to the genus Pseudomonas sensu stricto in molecular terms. The remaining 92 identified CSIs are specific for nine other Pseudomonas species clades and the genera Azomonas and Azotobacter which branch in between them. The identified CSIs provide strong independent evidence of the genetic cohesiveness of these species clades and offer reliable means for their demarcation/circumscription. Based on the robust phylogenetic and molecular evidence presented here supporting the distinctness of the observed Pseudomonas species clades, we are proposing the transfer of species from the following clades into the indicated novel genera: Alcaligenes clade – Aquipseudomonas gen. nov.; Fluvialis clade – Caenipseudomonas gen. nov.; Linyingensis clade – Geopseudomonas gen. nov.; Oleovorans clade – Ectopseudomonas gen. nov.; Resinovorans clade – Metapseudomonas gen. nov.; Straminea clade – Phytopseudomonas gen. nov.; and Thermotolerans clade – Zestomonas gen. nov. In addition, descriptions of the genera Azomonas, Azotobacter, Chryseomonas, Serpens, and Stutzerimonas are emended to include information for the CSIs specific for them. The results presented here should aid in the development of a more reliable classification scheme for Pseudomonas species.
Oral microbiome characteristics in patients with pediatric solid tumor
Authors: Cui Xichun , Du Xiaoran , Cui Xu , Fan Rongrong , Pan Juntao , Wang Zhifang
Published: January 2024

Background

Pediatric solid tumor, the abnormal proliferation of solid tissues in children resulting in the formation of tumors, represent a prevailing malignant ailment among the younger population. Extensive literature highlights the inseparable association linking oral microbiome and adult tumors, but due to differences in age of onset, characteristics of onset, etc., there are many differences between Pediatric solid tumors and adult tumors, and therefore, studying the relationship between Pediatric solid tumor and the oral microbiota is also essential.

Methods

To unravel the distinct characteristics of the oral microbiota within Pediatric solid tumor patients, 43 saliva samples, encompassing 23 Pediatric solid tumor patients and 20 healthy controls, were diligently procured. A meticulous screening process ensued, and conducted microbial MiSeq sequencing after screening.

Results

We documented the oral microbiome attributes among pediatric diagnosed with solid tumors (PST), and meanwhile, we observed a significant trend of decreased oral microbiota diversity in the pediatric solid tumor group. There were notable disparities in microbial communities observed between the two groups, 18 genera including Veillonellaceae, Firmicutes unclassified, Coriobacteriia, Atopobiaceae, Negativicutes, were significantly enriched in PST patients, while 29 genera, including Gammaproteobacteria, Proteobacteria, Burkholderiales, Neisseriaceae, were dominant in the HCs group. It was found that PST group had 16 gene functions, including Amino acid metabolism, Cysteine and methionine metabolism, Photosynthesis antenna proteins, Arginine and proline metabolism, and Aminoacyl tRNA biosynthesi, were significantly dominant, while 29 gene functions that prevailed in HCs.

Conclusion

This study characterized the oral microbiota of Pediatric solid tumor patients for the first time, and importantly, targeted biomarkers of oral microbiota may serve as powerful and non-invasive diagnostic tools for pediatric solid tumor patients.
Role of probiotics in managing various human diseases, from oral pathology to cancer and gastrointestinal diseases
Authors: Petrariu Oana-Alina , Barbu Ilda Czobor , Niculescu Adelina-Gabriela , Constantin Marian , Grigore Georgiana Alexandra , Cristian Roxana-Elena , Mihaescu Grigore , Vrancianu Corneliu Ovidiu
Published: January 2024

The imbalance of microbial composition and diversity in favor of pathogenic microorganisms combined with a loss of beneficial gut microbiota taxa results from factors such as age, diet, antimicrobial administration for different infections, other underlying medical conditions, etc. Probiotics are known for their capacity to improve health by stimulating the indigenous gut microbiota, enhancing host immunity resistance to infection, helping digestion, and carrying out various other functions. Concurrently, the metabolites produced by these microorganisms, termed postbiotics, which include compounds like bacteriocins, lactic acid, and hydrogen peroxide, contribute to inhibiting a wide range of pathogenic bacteria. This review presents an update on using probiotics in managing and treating various human diseases, including complications that may emerge during or after a COVID-19 infection.
The improvement of Hovenia acerba-sorghum co-fermentation in terms of microbial diversity, functional ingredients, and volatile flavor components during Baijiu fermentation
Authors: Zhang Jing , Zhao Minhui , Chen Jing , Zhu Yuanting , Xiao Chen , Li Qi , Weng Xiaoqi , Duan Yunxuan , Zuo Yong
Published: January 2024

The quality of Baijiu was largely affected by raw materials, which determine the flavor and taste. In the present study, organic acids, polyphenols, volatile flavor components and microbial community in Hovenia acerba-sorghum co-fermented Baijiu (JP1) and pure sorghum-fermented Baijiu (JP2) were comprehensively analyzed. Organic acids, polyphenols and volatile flavor components in JP1 were more abundant than JP2. The abundance and diversity of bacteria and fungi in JP1 was higher than that in JP2 in the early stage of fermentation, but presented opposite trend in the middle and late stages. Leuconostoc, Lentilactobacillus and Issatchenkia were dominant genera in JP1. Whereas, Cronobacter, Pediococcus and Saccharomyces occupied the main position in JP2. Lentilactobacillus and Issatchenkia were positively related to most of organic acids and polyphenols. Pseudomonas, Rhodococcus, Cronobacter, Pediococcus, Brucella, Lentilactobacillus, Lactobacillus, Saccharomycopsis, Wickerhamomyces, Aspergillus, Thermomyces and unclassified_f—Dipodascaccae were associated with the main volatile flavor components. The main metabolic pathways in two JPs exhibited the variation trend of first decreasing and then increasing, and the metabolism activity in JP1 were higher than that in JP2. The results demonstrated the introduction of Hovenia acerba improved the functional ingredients and volatile flavor components, which is helpful for the quality promotion of Baijiu. This study identified the key microorganisms and discussed their effect on organic acids, polyphenols and volatile flavor components during the fermentation of Baijiu with different raw materials, providing a scientific basis for the development and production of high-quality Baijiu.
Colonization and population dynamics of total, viable, and culturable cells of two biological control strains applied to apricot, peach, and grapevine crops
Authors: Daranas Núria , Badosa Esther , Montesinos Emilio , Bonaterra Anna
Published: January 2024

The ecological fitness of the biological control strains Bacillus velezensis A17 and Lactiplantibacillus plantarum PM411 was evaluated in different crops, geographical zones, and growing seasons. Both strains (2 g L−1 of dried formulation) were spray-inoculated on apricot trees, peach trees, and grapevines. Depending on the crop, flowers, fruits, and leaves were picked at several sampling time points. The population dynamics of viable, viable but non-culturable, and dead cells were studied by comparing viability qPCR (v-qPCR), qPCR, and plate counting estimations. A17 showed high survival rates in apricot, peach, and grapevine organs. The A17 viability was confirmed since qPCR and v-qPCR estimations did not significantly differ and were rather constant after field applications. However, higher population levels were estimated by plate counting due to the non-selective characteristics of the medium used. The viability of PM411 was constrained by plant organ, crop, and climate conditions, being higher in apricot than in grapevine. PM411 survival declined after field application, indicating difficulties in its establishment. The PM411 population level was made up of dead, culturable, and viable but non-culturable cells since significant differences between the three methods were observed. In conclusion, A17 and PM411 differ strongly in their survival in grapevine, peach, and apricot.