bluuebunny/crossref_metadata_2025 huggingface dataset:
Structure and Fungicidal Activity of Secondary Metabolites Isolated from Trichoderma hamatum b-3
Authors: Huang Li , Bian Qiang , Liu Mengdan , Hu Yiwen , Chen Lijuan , Gu Yucheng , Zu Qiwei , Wang Guangzhi , Guo Dale
Published: November 2024

Two new harziane diterpenes (1–2), five undescribed cyclonerane sesquiterpenes (3–7), and three known compounds, 11-cycloneren-3, 7, 10-triol (8), harziandione (9), and dehydroacetic acid (10), were isolated from Trichoderma hamatum b-3. Their structures were elucidated via comprehensive inspection of spectral evidence in HRESIMS and 1D and 2D NMR, and the absolute configuration of 1–8 was confirmed by NMR, ECD calculation, as well as Mosher’s method. In vitro fungicidal activity showed that some compounds showed great inhibitory activity against pathogenic fungi, including Fusarium graminearum, Sclerotinia sclerotiorum, Botrytis cinerea, and Rhizoctonia solani, among which compound 10 showed 100% inhibition of S. sclerotiorum and B. cinerea. The in vivo activity test showed that compound 10 was 65.8% effective against B. cinerea and compound 10 can be used as a lead compound for the development of biopesticides that inhibit B. cinerea. This study elucidated the bioactivity of secondary metabolites of T. hamatum and indicated the direction for the subsequent development of the biological control activity of T. hamatum.
Efficient Representative Volume Element of a Matrix–Precipitate Microstructure—Application on AlSi10Mg Alloy
Authors: Bouffioux Chantal , Papeleux Luc , Calvat Mathieu , Tran Hoang-Son , Chen Fan , Ponthot Jean-Philippe , Duchêne Laurent , Habraken Anne Marie
Published: November 2024

In finite element models (FEMs), two- or three-dimensional Representative Volume Elements (RVEs) based on a statistical distribution of particles in a matrix can predict mechanical material properties. This article studies an alternative to 3D RVEs with a 2.5D RVE approach defined by a one-plane layer of 3D elements to model the material behavior. This 2.5D RVE relies on springs applied in the out-of-plane direction to constrain the two lateral deformations to be compatible, with the goal of achieving the isotropy of the studied material. The method is experimentally validated by the prediction of the tensile stress–strain curve of a bi-phasic microstructure of the AlSi10Mg alloy. Produced by additive manufacturing, the sample material becomes isotropic after friction stir processing post treatment. If a classical plane strain 2D RVE simulation is clearly too stiff compared to the experiment, the predictions of the stress–strain curves based on 2.5D RVE, 2D RVE with no transversal constraint (called 2D free RVE), and 3D RVE simulations are close to the experiments. The local stress fields within a 2.5D RVE present an interesting similarity with 3D RVE local fields, but differences with the 2D free RVE local results. Since a 2.5D RVE simplifies one spatial dimension, the simulations with this model are faster than the 3D RVE (factor 2580 in CPU or taking into account an optimal parallel computation, a factor 417 in real time). Such a discrepancy can affect the FEM2 multi-scale simulations or the time required to train a neural network, enhancing the interest in a 2.5D RVE model.
Exercise as a Therapeutic Strategy for Obesity: Central and Peripheral Mechanisms
Authors: Zhang Yiyin , Wang Ruwen , Liu Tiemin , Wang Ru
Published: November 2024

Obesity is a complex, multifactorial condition involving excessive fat accumulation due to an imbalance between energy intake and expenditure, with its global prevalence steadily rising. This condition significantly increases the risk of chronic diseases, including sarcopenia, type 2 diabetes, and cardiovascular diseases, highlighting the need for effective interventions. Exercise has emerged as a potent non-pharmacological approach to combat obesity, targeting both central and peripheral mechanisms that regulate metabolism, energy expenditure, and neurological functions. In the central nervous system, exercise influences appetite, mood, and cognitive functions by modulating the reward system and regulating appetite-controlling hormones to manage energy intake. Concurrently, exercise promotes thermogenesis in adipose tissue and regulates endocrine path-ways and key metabolic organs, such as skeletal muscle and the liver, to enhance fat oxidation and support energy balance. Despite advances in understanding exercise’s role in obesity, the precise interaction between the neurobiological and peripheral metabolic pathways remains underexplored, particularly in public health strategies. A better understanding of these interactions could inform more comprehensive obesity management approaches by addressing both central nervous system influences on behavior and peripheral metabolic regulation. This review synthesizes recent insights into these roles, highlighting potential therapeutic strategies targeting both systems for more effective obesity interventions.
Integrated Metabolomic and Transcriptomic Analysis of Nitraria Berries Indicate the Role of Flavonoids in Adaptation to High Altitude
Authors: Zhao Qing , Zhang Jie , Li Yanhong , Yang Zufan , Wang Qian , Jia Qiangqiang
Published: November 2024

Background: Plants of Nitraria, belonging to the Zygophyllaceae family, are not only widely distributed at an altitude of about 1000 m but also at an altitude of about 3000 m, which is a rare phenomenon. However, little is known about the effect of altitude on the accumulation of metabolites in plants of Nitraria. Furthermore, the mechanism of the high–altitude adaptation of Nitraria has yet to be fully elucidated. Methods: In this study, metabolomics and transcriptomics were used to investigate the differential accumulation of metabolites of Nitraria berries and the regulatory mechanisms in different altitudes. Results: As a result, the biosynthesis of flavonoids is the most significant metabolic pathway in the process of adaptation to high altitude, and 5 Cyanidins, 1 Pelargonidin, 3 Petunidins, 1 Peonidin, and 4 Delphinidins are highly accumulated in high–altitude Nitraria. The results of transcriptomics showed that the structural genes C4H (2), F3H, 4CL (2), DFR (2), UFGT (2), and FLS (2) were highly expressed in high–altitude Nitraria. A network metabolism map of flavonoids was constructed, and the accumulation of differential metabolites and the expression of structural genes were analyzed for correlation. Conclusions: In summary, this study preliminarily offers a new understanding of metabolic differences and regulation mechanisms in plants of Nitraria from different altitudes.
An Amperometric Sensor with Anti-Fouling Properties for Indicating Xylazine Adulterant in Beverages
Authors: Vinnikov Arielle , Sheppard Charles W. , Wemple Ann H. , Stern Joyce E. , Leopold Michael C.
Published: November 2024

Amperometric electrochemical sensing schemes, which are easily fabricated and can directly relate measured current with analyte concentrations, remain a promising strategy for the development of the portable, in situ detection of commonly employed adulterants. Xylazine (XYL) is a non-narcotic compound designed for veterinary use as a sedative known as Rompun®. XYL is increasingly being abused as a recreational drug, as an opioid adulterant and, because of its chemical properties, has found unfortunate prominence as a date rape drug spiked into beverages. In this study, a systematic exploration and development of fouling-resistant, amperometric XYL sensors is presented. The sensing strategy features layer-by-layer (LBL) modification of glassy carbon electrodes (GCEs) with carbon nanotubes (CNTs) for sensitivity and the engagement of cyclodextrin host–guest chemistry in conjunction with polyurethane (PU) semi-permeable membranes for selectivity. The optimization of different materials and parameters during development created a greater fundamental understanding of the interfacial electrochemistry, allowing for a more informed subsequent design of effective sensors exhibiting XYL selectivity, effective sensitivity, rapid response times (<20 s), and low estimated limits of detection (~1 ppm). Most importantly, the demonstrated XYL sensors are versatile and robust, easily fabricated from common materials, and can effectively detect XYL at <10 ppm in both common alcoholic and non-alcoholic beverages, requiring only minimal volume (20 µL) of the spiked beverage for a standard addition analysis.
Investigation of Pozzolan Activity, Chemical and Granulometric Composition of Micro- and Nanosilicon of the Bratsk Ferroalloy Plant
Authors: Karlina Antonina I. , Karlina Yuliya I. , Gladkikh Vitaliy A.
Published: November 2024

The article presents the findings of a study conducted on a range of microsilicon grades selected at the Bratsk Ferroalloy Plant. The following analytical techniques were employed: X-ray fluorescence analysis, X-ray diffraction analysis, a granulometric composition study, and pozzolanic properties. The grades of the investigated microsilicon are compared with the furnace grade and the grade of the produced ferrosilicon. The findings of the research conducted at the Bratsk Ferroalloy Plant indicate that the microsilicon produced at the facility is suitable for use as an additive in the production of tires, artificial irregularities, and other rubber products intended for use on roads. In such applications, the quality and durability of the material are determined by its ability to withstand abrasion and wear. Therefore, it is essential to utilize the purest, most amorphous, and most finely dispersed silicon dioxide. The gas cleaning device GCD-4 FeSi-75 exhibits the greatest number of these parameters among the samples presented. Different samples of microsilica have a color from white to dark gray. The chemical and granulometric compositions were determined. The pozzolan activity was investigated. Based on the conducted analyses, it is possible to draw conclusions about the properties of materials and the potential for use in the construction industry for concretes of various values. The results of the analyses indicate that silicon dioxide with GCD-4 FeSi-75 is suitable for use in critical concrete structures. The quality of the silicon dioxide with GCD-4 FeSi-75 can be compared with that of Elkem 971. It is recommended that all the studied samples be employed as modifiers for cast iron, with the GCD-4 FeSi-75 sample being the optimal choice for testing in steels. The utilization of this modifier enables a reduction in the consumption of FeSi, exerting both an alloying and modifying effect on the melt. However, it is essential to emphasize the necessity for technological selection of the method of administration, as the powder, in its pure form, is susceptible to combustion and is not readily digestible. The quality of such a modifier, with a stable guaranteed effect, is comparable to the use of FeSi. Silicon dioxide plays an essential role in the production of refractories. The primary criteria for this industry are purity, the minimum content of the crystalline phase, and the activity of the material. It is recommended that the material from GCD-4 FeSi-75 be used in the production of refractories.
Construction and Evaluation of Hepatic Targeted Drug Delivery System with Hydroxycamptothecin in Stem Cell-Derived Exosomes
Authors: Zhao Qiongjun , Mo Zixuan , Zeng Liuting , Yuan Yue , Wang Yan , Wang Ying
Published: November 2024

Hydroxycamptothecin (HCPT) is commonly used in the treatment of liver cancer; however, its low water solubility and poor stability significantly limit its clinical application. In recent years, research on exosomes has deepened considerably. Exosomes possess a unique phospholipid bilayer structure, enabling them to traverse tissue barriers, which provides natural advantages as drug carriers. Nevertheless, delivering exosomes safely and efficiently to target cells remains a major challenge. In this study, we utilized the affinity of the SP94 peptide for human liver cancer cell receptors. HCPT was coated with exosomes in our experimental design, and the exosome membrane was modified with SP94 peptide to facilitate drug delivery to liver cancer cells. Exosomes were purified from bone marrow mesenchymal stem cells, and targeted peptides were attached to their surfaces via post-insertion techniques. Subsequently, HCPT was incorporated into the exosomes through electroporation. Using the HepG2 hepatoma cell line, we evaluated a series of in vitro pharmacodynamics and studied pharmacokinetics and tissue distribution in animal models. The results indicated that ligand-targeted, modified drug-carrying exosomes significantly enhance drug bioavailability, prolong retention time in vivo, and facilitate liver targeting. Moreover, this approach reduces drug nephrotoxicity, enhances anti-tumor efficacy, and lays the groundwork for the development of novel liver cancer-targeting agents.
Monoterpene Hydroxy Lactones Isolated from Thalassiosira sp. Microalga and Their Antibacterial and Antioxidant Activities
Authors: Morais Alcina M. M. B. , Kumla Decha , Martins Valter F. R. , Alves Ana , Gales Luis , Silva Artur M. S. , Costa Paulo M. , Mistry Sharad , Kijjoa Anake , Morais Rui M. S. C.
Published: November 2024

Two monoterpenoid lactones, loliolide (1) and epi-loliolide (2), were isolated from the crude dichloromethane extract of a microalga, Thalassiosira sp.). The structures of loliolide (1) and epi-loliolide (2) were elucidated by 1D and 2D NMR analysis, as well as a comparison of their 1H or/and 13C NMR data with those reported in the literature. In the case of loliolide (1), the absolute configurations of its stereogenic carbons were confirmed by X-ray analysis, whereas those of epi-loliolide (2) were determined by NOESY correlations. Loliolide (1) and epi-loliolide (2) were tested for their growth inhibitory activity against two Gram-positive (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212) and two Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) bacteria, as well as one clinical isolate (E. coli SA/2, an extended-spectrum β-lactamase producer-ESBL) and two environmental isolates, S. aureus 74/24, a methicillin-resistant (MRSA), and E. faecalis B3/101, a vancomycin-resistant (VRE) isolates. The results showed that none of the tested compounds exhibited antibacterial activity at the highest concentrations tested (325 μM), and both revealed low antioxidant activity, with ORAC values of 2.786 ± 0.070 and 2.520 ± 0.319 µmol TE/100 mg for loliolide (1) and epi-loliolide (2), respectively.
Elucidation of the Effect of Solar Light on the Near-Infrared Excitation Raman Spectroscopy-Based Analysis of Fabric Dyes
Authors: Bober Shannon , Kurouski Dmitry
Published: November 2024

Colored textiles are valuable physical evidence often found at crime scenes. Analysis of the chemical structure of textiles could be used to establish a connection between fabric found at a crime scene and suspect cloths. High-performance liquid chromatography (HPLC) and mass spectroscopy coupled HPLC are traditionally used for the identification of dyes in fabric. However, these techniques are invasive and destructive. A growing body of evidence indicates that near-infrared excitation (λ = 830 nm) Raman spectroscopy (NIeRS) could be used to probe the chemical signature of such colorants. At the same time, it remains unclear whether environmental factors, such as solar light could lower the accuracy of NIeRS-based identification of dyes in textiles. In this study, we exposed cotton fabric colored with six different dyes to light and investigated the extent to which colorants fade during seven weeks using NIeRS. We found a decrease in the intensities of all vibrational bands in the acquired spectra as the time of the exposition of fabric to light increased. Nevertheless, utilization of partial least-squared discriminant analysis (PLS-DA) enabled identification of the colorants at all eight weeks. These results indicate that the effect of light exposure should be strongly considered by forensic experts upon the NIeRS-based analysis of colored fabric.
Nitrogen and Sulfur Co-Doped Graphene-Quantum-Dot-Based Fluorescent Sensor for Rapid Visual Detection of Water Content in Organic Solvents
Authors: Zhang Hongyuan , Wang Jieqiong , Ji Xiaona , Bao Yanru , Han Ce , Sun Guoying
Published: November 2024

Accurate water content detection is crucial for optimizing chemical reactions, ensuring product quality in pharmaceutical manufacturing, and maintaining food safety. In this study, nitrogen and sulfur co-doped graphene quantum dots (R-GQDs) were synthesized via a one-step hydrothermal method using o-phenylenediamine as the carbon source. The synthesis conditions, including reaction time, temperature, o-phenylenediamine concentration, and H2SO4/water ratio, were optimized using the Box-Behnken response surface methodology. The R-GQDs exhibited excellent fluorescence stability and distinct solvent-dependent characteristics, alongside a broad linear detection range and high sensitivity, making them highly suitable for dual-mode water content detection (colorimetric and fluorescent). To enhance the accuracy of visual detection, R-GQDs were incorporated into portable test strips with smartphone-assisted analysis, compensating for the human eye’s limitations in distinguishing subtle color changes. The sensor’s practical utility was validated through spiked recovery experiments in food samples, and the R-GQDs demonstrated good biocompatibility for in vivo imaging in shrimp. These findings highlight a novel strategy for developing portable, real-time water content sensors with potential applications in both portable detection systems and biological imaging.
The Composition, Antioxidant and Antibacterial Activity of Essential Oils from Five Species of the Magnoliaceae Family
Authors: Yang Dandan , Ma Daocheng , Song Ziqi , Yang Mei , Xu Yuanyuan
Published: November 2024

The leaves of Magnoliaceae family plants contain abundant essential oils (EOs), and these species can be used in many fields due to their high industrial, medicinal, and ornamental values. This study aims to identify the main compounds of the EOs from the leaves of five common Magnoliaceae species (Michelia maudiae, Michelia hedyosperma, Michelia macclurei, Manglietia lucida, Manglietia conifer) using hydrodistillation, GC–MS analysis, and in vitro tests. Additionally, the antioxidant and antibacterial activities of the EOs were also evaluated. The results show that 151 compounds were identified across five species, with sesquiterpenes being dominant. Some key compounds (such as β-caryophyllene, δ-amorphene, β-guaiene, globulol, and β-acorenol) were common among all the species, highlighting their crucial roles in plant physiology and resilience. Other compounds (like valeranone and nerolidol in M. maudiae and β-elemene in M. macclurei) were specific, indicating different functions. Among the five species, the essential oil of M. macclurei contains β-elemene and nerolidyl acetate, and it has the weakest antioxidant activity (IC50 value: 2918.61~21,341.98 μg/mL) but the strongest antibacterial activity (inhibition zone diameter: 8.55 ± 0.93~22.92 ± 0.46 mm; LC50 value: 0.02~0.78 mg/mL). Meanwhile, the EO of M. maudiae demonstrated the best antioxidant activity (the IC50 value was 1283.58~6258.32 μg/mL) and the second-best antibacterial activity (the inhibition zone diameter ranged from 7.61 ± 0.02 to 26.92 ± 0.46 mm, and the LC50 value was 0.03~2.28 mg/mL). Overall, the EO of M. maudiae had the best comprehensive performance. Therefore, the EOs of M. macclurei and M. maudiae showed different performances in biological activity categories, and they could be developed and used in different fields, with the possibility of discovering new applications. This brings inspiration to the potential commercial and industrial uses of sesquiterpenes in Magnoliaceae.
First-Principles Study on Strain-Induced Modulation of Electronic Properties in Indium Phosphide
Authors: Yan Libin , Chen Zhongcun , Bai Yurong , Liu Wenbo , He Huan , He Chaohui
Published: November 2024

Indium phosphide (InP) is widely utilized in the fields of electronics and photovoltaics due to its high electron mobility and high photoelectric conversion efficiency. Strain engineering has been extensively employed in semiconductor devices to adjust physical properties and enhance material performance. In the present work, the band structure and electronic effective mass of InP under different strains are investigated by ab initio calculations. The results show that InP consistently exhibits a direct bandgap under different strains. Both uniaxial strain and biaxial tensile strain exhibit linear effects on the change in bandgap values. However, the bandgap of InP is significantly influenced by uniaxial compressive strain and biaxial tensile strain, respectively. The study of the InP bandgap under different hydrostatic pressures reveals that InP becomes metallic when the pressure is less than −7 GPa. Furthermore, strain also leads to changes in effective mass and the anisotropy of electron mobility. The studies of electronic properties under different strain types are of great significance for broadening the application of InP devices.
Recent Progress in Two-Dimensional Magnetic Materials
Authors: Shi Guangchao , Huang Nan , Qiao Jingyuan , Zhang Xuewen , Hu Fulong , Hu Hanwei , Zhang Xinyu , Shang Jingzhi
Published: November 2024

The giant magnetoresistance effect in two-dimensional (2D) magnetic materials has sparked substantial interest in various fields; including sensing; data storage; electronics; and spintronics. Their unique 2D layered structures allow for the manifestation of distinctive physical properties and precise performance regulation under different conditions. In this review, we present an overview of this rapidly developing research area. Firstly, these 2D magnetic materials are catalogued according to magnetic coupling types. Then, several vital effects in 2D magnets are highlighted together with theoretical investigation, such as magnetic circular dichroism, magneto-optical Kerr effect, and anomalous Hall effect. After that, we forecast the potential applications of 2D magnetic materials for spintronic devices. Lastly, research advances in the attracting magnons, skyrmions and other spin textures in 2D magnets are discussed.
Mitigating Dietary Bisphenol Exposure Through the Gut Microbiota: The Role of Next-Generation Probiotics in Bacterial Detoxification
Authors: Emanowicz Paulina , Średnicka Paulina , Wójcicki Michał , Roszko Marek , Juszczuk-Kubiak Edyta
Published: November 2024

Bisphenols, such as bisphenol A and its analogs, which include bisphenol S, bisphenol F, bisphenol AF, and tetramethyl bisphenol F, are chemical contaminants commonly found in food that raise serious health concerns. These xenobiotics can potentially have harmful effects on human health. The gut microbiota plays a crucial role in metabolizing and neutralizing these substances, which is essential for their detoxification and elimination. Probiotic supplementation has been studied for its ability to modulate the gut microbiota’s composition and function, enhancing detoxification processes. Next-Generation Probiotics (NGPs) may exhibit better properties than traditional strains and are designed for targeted action on specific conditions, such as obesity. By modulating inflammatory responses and reducing the secretion of pro-inflammatory cytokines, they can significantly improve host health. Research on NGPs’ ability to neutralize obesogenic bisphenols remains limited, but their potential makes this a promising area for future exploration. This review aims to understand the mechanisms of the chemical transformation of bisphenol through its interactions with the gut microbiota and the role of probiotics, particularly NGPs, in these processes. Understanding the interplay between bisphenols, gut microbiota, and NGPs may pave the way for strategies to counteract the negative health effects associated with daily and chronic exposure to bisphenols, which is crucial for food safety and consumer health protection.
How Follow-Up Period in Prospective Cohort Studies Affects Relationship Between Baseline Serum 25(OH)D Concentration and Risk of Stroke and Major Cardiovascular Events
Authors: Grant William B. , Boucher Barbara J.
Published: November 2024

Background/Objectives: Prospective cohort studies are useful for studying how biomolecular status affects risk of adverse health outcomes. Less well known is that the longer the follow-up time, the lower the association (or “apparent effect”) due to “regression dilution”. Here, we evaluate how follow-up interval from baseline to “event” affects the relationship between baseline serum 25-hydroxyvitamin D [25(OH)D] concentration and the later incidence of stroke and major cardiovascular events (MACEs). Methods: Findings for the relative risk (RR) of stroke and MACEs with respect to serum 25(OH)D concentrations at baseline from prospective cohort studies were plotted against mean follow-up time. Fifteen studies from mainly European countries and the United States were used for stroke and nine studies for MACEs. Linear regression analyses were used to study data for follow-up periods of up to 10 years and for more than 10 years. Results: For stroke, the linear regression fit for 1–10 years is RR = 0.34 + (0.065 × follow-up [years]), r = 0.84, adjusted r2 = 0.67, p < 0.001. No significant variations in association were found for studies with follow-up periods of 10–20 years. For MACEs, the linear fit for 1–8.1 years is RR = 0.61 + (0.055 × follow-up [years]), r = 0.81, adjusted r2 = 0.59, p = 0.03. Discussion: The shorter the follow-up period, the greater the apparent effect of better vitamin D status in reducing risk of stroke and MACEs. In addition, the apparent effect of higher 25(OH)D concentration found for the shortest follow-up time is more than twice as great as the estimate based on average follow-up intervals for all studies. Mechanisms have been found to explain how higher serum 25(OH)D concentrations could reduce risk of stroke and MACEs. Randomized controlled trials have not shown that vitamin D supplementation significantly reduces risk of either stroke or MACEs, probably because risk of both outcomes increases rapidly below 15 ng/mL (38 nmol/L) and it is difficult in Western developed countries to enroll enough participants with concentrations that low. Nonetheless, vitamin D’s role in reducing risk of stroke and MACEs could be considered causal on the basis of an evaluation of the evidence using Hill’s criteria for causality in a biological system. Conclusions: Serum 25(OH)D concentrations above 20 ng/mL are associated with significantly reduced risk of stroke and MACEs prospectively and in an apparent causal manner. Raising serum 25(OH)D concentrations to >20 ng/mL should, therefore, be recommended for everyone likely to be at risk for stroke or MACEs and indeed in the general population.
Effect of Acacia concinna Extract on Apoptosis Induction Associated with Endoplasmic Reticulum Stress and Modulated Intracellular Signaling Pathway in Human Colon HCT116 Cancer Cells
Authors: Sitthisuk Pornnapa , Innajak Sukanda , Poorahong Watcharaporn , Samosorn Siritron , Dolsophon Kulvadee , Watanapokasin Ramida
Published: November 2024

Background: Colorectal cancer (CRC) stands as one of the most prevalent cancer types and among the most frequent causes of cancer-related death globally. Acacia concinna (AC) is a medicinal and edible plant that exhibits a multitude of biological properties, including anticancer properties. This study aimed to investigate the impact of the AC extract on apoptosis induction and the underlying mechanisms associated with this effect in KRAS-mutated human colon HCT116 cells. Methods: The effect of AC extract on cell cytotoxicity was evaluated using MTT assay. Nuclear morphological changes were visualized with Hoechst 33342 staining, while mitochondrial membrane potential (MMP) was assessed via JC-1 staining. Flow cytometry was employed for cell cycle analysis, and intracellular ROS levels were determined using DCFH-DA staining. Results: The results showed that HCT116 cells exposed to AC extract showed reduced cell growth and prompted apoptosis, as indicated by an increase in chromatin condensation, apoptotic bodies, the sub-G1 apoptotic cell population, and disrupted MMP. Expression levels of apoptosis mediator proteins determined by Western blot analysis showed an increase in pro-apoptotic proteins (Bak and Bax) while decreasing anti-apoptotic proteins (Bcl-2, Bcl-xL, and Mcl-1), leading to caspase-7 activation and PARP inactivation. AC extract was also found to enhance intracellular reactive oxygen species (ROS) levels and stimulate endoplasmic reticulum (ER) stress. Furthermore, AC extract increases the phosphorylation of ERK1/2, p38, and c-Jun while downregulating PI3K, Akt, β-catenin, and their downstream target proteins. Conclusions: These results demonstrate that AC extract could inhibit cancer cell growth via ROS-induced ER stress associated with apoptosis and regulate the MAPK, PI3K/Akt, and Wnt/β-catenin signaling pathways in HCT116 cells. Therefore, AC extract may be a novel candidate for natural anticancer resources for colon cancer treatment.
Employer Actions in Office Settings and Women’s Perception of the Workplace as Supportive of Healthy Eating: A Cross-Sectional Pilot Study
Authors: Hyży Aleksandra , Cieślak Ilona , Gotlib-Małkowska Joanna , Panczyk Mariusz , Kucharska Alicja , Jaworski Mariusz
Published: November 2024

Background/Objectives: This study aimed to evaluate how women working in office environments perceive their workplace as promoting healthy eating behaviors through employer-led actions. Methods: This cross-sectional study was conducted among 230 professionally active women employed in office settings in Poland. Data were collected using the Computer-Assisted Web Interview (CAWI) method. Participants were divided into two groups based on their perceived level of workplace support for healthy eating behaviors, as measured by the Workplace Healthy Eating Scale. Group 1 (n = 125; 54.3%; mean score = 15.69, SD = 3.76) and Group 2 (n = 105; 45.7%; mean score = 29.88, SD = 5.15) reflected low and high perceived support, respectively. Results: A linear regression model was employed to assess the association between the perceived level of support and specific workplace initiatives, including access to fresh fruits and vegetables, meal preparation facilities, cafeteria usage, lectures on nutrition, cooking workshops, and individual dietary consultations. For Group 1, access to fresh fruits and vegetables was the only factor significantly associated with a positive perception of the workplace as promoting healthy eating (p = 0.003), explaining 6.5% of the variance (adjusted R2 = 0.065). In Group 2, both access to fresh produce and participation in cooking workshops were significantly associated with positive workplace perceptions (p < 0.001), explaining 41% of the variance (adjusted R2 = 0.410). Conclusions: Access to fresh produce is a key determinant of employees’ perceptions of workplace support for healthy eating behaviors, with a notably greater impact observed when combined with additional activities such as cooking workshops. Employer-led initiatives focusing on practical dietary engagement appear to be effective in enhancing workplace perceptions of health promotion.
Nebivolol, an FDA-Approved Drug, Has General Antibacterial and Antibiofilm Effects and Increases Pseudomonas aeruginosa Tolerance to Ciprofloxacin
Authors: Schlichter Kadosh Yael , Goorevitch Noa , Teralı Kerem , Gopas Jacob , Kushmaro Ariel
Published: November 2024

Background: The repurposing of approved drugs for new activities is gaining widespread attention, including drugs that have antibacterial properties. Nevertheless, besides the benefits of repurposing drugs, the discovery of new antibiotic activity in commonly used medicines raises concerns about inducing antibiotic tolerance and resistance due to the stress produced by the drugs. We found that nebivolol, which is used to treat hypertension, also has antibacterial activity. Methods: The antibacterial activity of nebivolol was tested by disc diffusion and kinetic O.D. measurements. Antibiofilm activity was determined by crystal violet staining. Results: Nebivolol has antibiotic and antibiofilm activity against several bacteria. However, its effect on Pseudomonas aeruginosa’s growth is limited, and it promotes biofilm formation. In addition, P. aeruginosa exposure to nebivolol induces resistance to ciprofloxacin but increases sensitivity to tobramycin. Conclusions: Nebivolol has antibiotic activity against several bacteria tested but is less effective and possibly detrimental in P. aeruginosa infections. The use of nebivolol, together with other antibiotics, should be further tested and carefully considered.
The Role of Pharmacogenetic-Based Pharmacokinetic Analysis in Precise Breast Cancer Treatment
Authors: Wu Xinyu , Xiong Huihua
Published: November 2024

Given the high prevalence of breast cancer and the diverse genetic backgrounds of patients, a growing body of research emphasizes the importance of pharmacogenetic-based pharmacokinetic analysis in optimizing treatment outcomes. The treatment of breast cancer involves multiple drugs whose metabolism and efficacy are influenced by individual genetic variations. Genetic polymorphisms in drug-metabolizing enzymes and transport proteins are crucial in the regulation of pharmacokinetics. Our review aims to investigate the opportunities and challenges of pharmacogenomic-based pharmacokinetic analysis as a precision medicine tool in breast cancer management.
High-Efficiency and High-Monochromaticity Semitransparent Organic Solar Cells Based on Optical Tamm States
Authors: Zhao Junwei , Lin Senxuan , Zhou Jinxin , Gao Fuhao , Liu Jingfeng , Long Yongbing , Xu Haitao
Published: November 2024

Semitransparent organic solar cells (ST-OSCs) have garnered more interest and stand out as promising candidates for next-generation solar energy harvesters with their unique advantages. However, challenges remain for the advancement of colorful ST-OSCs, such as enhancing the light absorption and transmittance without considerable power conversion efficiency (PCE) losses. Herein, an optical analysis of silver (Ag) electrodes and one-dimensional photonic crystals (1DPCs) was conducted by simulations, revealing the presence of optical Tamm states (OTSs) at the interface of Ag/1DPCs. Furthermore, the spectral and electrical properties were fine-tuned by modulating the OTSs through theoretical simulations, utilizing PM6:Y6 as the active layer. The structural parameters of the ST-OSCs were optimized, including the Ag layer thickness, the central wavelength of 1DPCs, the first WO3 layer thickness, and the pair number of WO3/LiF. The optimization resulted in the successful development of blue, violet-blue, and red ST-OSC devices, which exhibited transmittance peak intensities ranging from 31.5% to 37.9% and PCE losses between 1.5% and 5.2%. Notably, the blue device exhibited a peak intensity of 37.0% and a PCE of 15.24%, with only a 1.5% loss in efficiency. This research presents an innovative approach to enhancing the performance of ST-OSCs, achieving a balance between high transparency and high efficiency.
Wavelength-Switchable 2 μm Single-Longitudinal-Mode Thulium-Doped Fiber Laser Based on Dual-Active Cavity and DLTCTR
Authors: Wang Pengfei , Qin Qi , Yan Fengping , Yang Dandan , Yu Chenhao , Hu Junjie , Cao Xiqing , Xu Darui , Liu Peng , Guan Biao , Guo Ying
Published: November 2024

A thulium-doped fiber laser (TDFL) with a dual-active cavity and a directly linked three-coupler triple-ring filter is designed and demonstrated. Its operational principle is analyzed, and a corresponding experimental setup is built. Eleven single-wavelength laser outputs with a single-longitudinal-mode (SLM) output near 2 μm are obtained. The laser output covers a wavelength range from 1933.95 nm to 1971.76 nm, with a continuous switchable output range of 37.81 nm and a minimum center wavelength interval of 0.22 nm. The optical signal-to-noise ratio (OSNR) of the output laser within the tuning range is >48.53 dB, and its maximum OSNR is 70.24 dB. The minimum wavelength fluctuation is 0.03 nm, and the power fluctuation is between 0.15 and 2.61 dB. A single wavelength with a center wavelength of 1933.95 nm is monitored for 75 min, and the radio-frequency spectrum is scanned 27 times within the frequency range of 0 to 400 MHz. The results demonstrate that the TDFL can operate continuously and stably in an SLM state. The linewidth and linewidth fluctuation of the TDFL are measured, and the minimum linewidth, corresponding to a measurement time of 0.001 s, is 65.14 kHz. The experimental results show that the proposed TDFL has a high OSNR and excellent wavelength-switching ability, and its SLM operation is very stable.
Fault-Tolerant Scheduling Mechanism for Dynamic Edge Computing Scenarios Based on Graph Reinforcement Learning
Authors: Zhang Yuze , Xia Geming , Yu Chaodong , Li Hongcheng , Li Hongfeng
Published: November 2024

With the proliferation of Internet of Things (IoT) devices and edge nodes, edge computing has taken on much of the real-time data processing and low-latency response tasks which were previously managed by cloud computing. However, edge computing often encounters challenges such as network instability and dynamic resource variations, which can lead to task interruptions or failures. To address these issues, developing a fault-tolerant scheduling mechanism is crucial to ensure that a system continues to operate efficiently even when some nodes experience failures. In this paper, we propose an innovative fault-tolerant scheduling model based on asynchronous graph reinforcement learning. This model incorporates a deep reinforcement learning framework built upon a graph neural network, allowing it to accurately capture the complex communication relationships between computing nodes. The model generates fault-tolerant scheduling actions as output, ensuring robust performance in dynamic environments. Additionally, we introduce an asynchronous model update strategy, which enhances the model’s capability of real-time dynamic scheduling through multi-threaded parallel interactions with the environment and frequent model updates via running threads. The experimental results demonstrate that the proposed method outperformed the baseline algorithms in terms of quality of service (QoS) assurance and fault-tolerant scheduling capabilities.
Isolation, Purification, and Antioxidant Activity of Polyphenols from Cynanchum auriculatum Royle ex Wight
Authors: Nasiru Mustapha Muhammad , Sun Yue-E , Zhao Lingyun , Bunhok Taing , Roth Chuon Mony , Sovath Sun , Pharith Hay , Wang Weidong , Li Chunyang
Published: November 2024

Cynanchum auriculatum Royle ex Wight (CA) is a traditional medicinal and edible plant in China. This study aimed to isolate and characterize the phenolic compounds of C. auriculatum to identify its main antioxidant constituents. Polyphenols were extracted using an ultrasound-assisted ethanol extraction method, followed by partitioning with ethyl acetate. The ethyl acetate extract was then purified through thin-layer chromatography, silica gel column chromatography, and reverse-phase silica gel column chromatography. Three monomeric compounds—cynandione A (I), 2,5-dihydroxyacetophenone (II), and radix piperacanthone (III)—were identified through their physical and chemical properties, UV and IR spectra, and liquid chromatography–mass spectrometry (LC-MS/MS). Vitamin C (VC) and 2,4-dihydroxyacetophenone were used as controls to evaluate the antioxidant potential of the two most abundant monomers. Antioxidant assays demonstrated that 2,5-dihydroxyacetophenone and cynandione A exhibited strong antioxidant activity at lower concentrations, whereas 2,4-dihydroxyacetophenone showed significantly weaker activity. Furthermore, cynandione A displayed superior cellular antioxidant activity compared to 2,5-dihydroxyacetophenone, indicating its potential as a promising bioactive compound. In conclusion, this study provides valuable insights into the phenolic composition of C. auriculatum and highlights cynandione A as a key antioxidant, paving the way for future research on its therapeutic applications.
The 15-Minute City: An Attempt to Measure Proximity to Urban Services in Rome
Authors: Chiaradia Francesco , Lelo Keti , Monni Salvatore , Tomassi Federico
Published: November 2024

This paper aims to assess the proximity of urban services through the lens of the 15 min city concept, using the Municipality of Rome as a case study. The question of whether Rome qualifies as a 15 min city is explored by examining proximity in terms of the “intensity” of urban services accessible within a 15 min walking or cycling distance. A simple model, based on GIS techniques, is implemented to measure proximity to urban services at the neighbourhood level. Furthermore, the proximity levels in urban areas characterised by similar socioeconomic conditions, referred to as the “seven cities” of Rome, are analysed to identify potential inequalities. Our analysis shows that the dense and walkable Rome known by tourists is not the place most Romans live in.
Analysis of Spatiotemporal Disparities and Spatial Spillover Effect of a Low-Carbon Economy in Chinese Provinces Under Green Technology Innovation
Authors: Liu Xiu , He Zhuo , Deng Zixin , Poddar Sandeep
Published: November 2024

This research, set against the backdrop of rapid global advancements in green technology innovation and aligned with China’s sustainable development strategy, conducts an in-depth analysis of the spatiotemporal disparities and spatial spillover effect of a low-carbon economy in Chinese provinces. Following empirical analysis and data testing, the study draws the following conclusions: (1) China’s low-carbon economy is subject to significant regional differences. Over time, the development level has improved annually, with the number of provinces above the middle level of low-carbon economy rising from 11 in 2010 to 22 in 2022. However, regional differences persist. The eastern region has shown the most significant progress, while the central area encountered its initial challenges first, and the development of the western area has unfolded in a more segmented manner. (2) In this paper, the low-carbon economy in China shows notable characteristics of spatiotemporal agglomeration, with the number of high–high agglomeration provinces rising from four in 2010 to seven in 2022, an increase of 78% year-on-year. The number of provinces and provinces in the low–low agglomeration mode increased from two in 2010 to six in 2022, a year-on-year increase of 200%. (3) China’s low-carbon economy demonstrates notable spatiotemporal evolution. The development process tends to remain stable during transitions, with a high likelihood of sustaining the current state while gradually advancing, avoiding abrupt changes across levels. (4) Innovations in green technology exert a positive influence on the advancement of the low-carbon economy, with a clear spatial spillover effect. In light of these findings, the research offers targeted recommendations for society, enterprises, and government stakeholders.
Operational Energy in Historic Religious Buildings: A Qualitative Approach
Authors: Berthold Étienne , Pawliw Kim , St-Pierre Mathieu , Pronovost Francis , Méthé Léa
Published: November 2024

Typically, operational energy is approached and evaluated from a quantitative point of view and, to a large extent, according to life cycle assessment (LCA). This article seeks to develop a qualitative approach to assess the past operational energy of a historic religious building in the province of Quebec, Canada. We propose a method for determining the past thermal sensation of individuals residing in a monastery by evaluating this sensation using the thermal sensation vote (TSV) related to the predicted mean vote (PMV). Doing so allows us to infer the operational temperatures and setpoints, providing an additional indicator of energy consumption. The proposed method is based on the identification and analysis of individual perceptions contained in archive documents, facilitating the reconstruction of the expressed thermal sensation and of a TSV index. The method is deployed on a prospective basis, enabling the creation of a chronological series designed to exhaustively document the thermal sensation during heating periods. This article contributes to discussions among critics who have observed a mismatch between TSV indices and PMV parameters and prognosis. It also brings us closer to a finer understanding of thermal comfort and the use/consumption of operational energy in historic religious buildings.
Assessing the Influence of Digital Inclusive Finance on Household Financial Vulnerability in China: Insights from Health Insurance Participations
Authors: Liu Shuyan , Feng Yulin (Frank) , Ye Meiqi
Published: November 2024

Poverty reduction is the primary goal of the United Nations 2030 Agenda for Sustainable Development. Enhancing the purchase rate of health insurance is essential for alleviating poverty caused by health shocks, as it serves as a crucial risk management tool for addressing health-related risks. In this paper, we investigate the impact of digital inclusive finance on household participation in terms of health insurance and financial vulnerability, utilizing the Digital Inclusive Finance Index developed by Peking University and survey data from the China Household Finance Survey. Our findings indicate that the advancement of digital inclusive finance can significantly reduce the risk of household financial vulnerability by increasing household health insurance enrollment rate. The findings are robust across various digital inclusive finance indices, different metrics for financial vulnerability, alternative econometric models, and additional control variables. Furthermore, the effects of digital inclusive finance on health insurance enrollments and household financial vulnerability are particularly pronounced among urban households and those led by younger and more risk-averse household heads. Our findings advocate for further development of digital inclusive finance, mainly targeted at rural households and those with elderly heads, to enhance health insurance participation and mitigate the risk of illness-related poverty.
The Impact of Internal Service Quality and Ethical Leadership on Employee Performance
Authors: Aydemir Sibel , Kıpçak Emrullah
Published: November 2024

One of the most fundamental values that businesses must ensure to enhance sustainable production and productivity is the satisfaction of their internal customers. It is anticipated that an increase in the satisfaction levels of internal customers will lead to improved work performance, creating a cycle linked to the services and values provided to employees by the organization. This study aims to uncover the impact of the quality of internal services offered by manufacturing companies, as well as the ethical leadership approach, on employee performance. The data obtained from surveys conducted with 412 employees of clothing and textile companies in Van, Turkey, were analyzed using the SPSS program. The results indicate that internal service quality, particularly its dimensions of responsiveness and assurance, positively affects employee performance. Furthermore, it was observed that ethical leadership generally has a negative effect on employee performance, but the ethicality and justice dimensions and task clarity dimensions of ethical leadership affect employee performance positively.