bluuebunny/crossref_metadata_2025 huggingface dataset:
Distribuição e autocorrelação espacial dos casos de Tentativas de Suicídio por medicamentos em um município paraibano
Authors: Farias Andreza Josiany Aires de , Moraes Allana Renally Cavalcante Santos de , Olinda Ricardo Alves de , Mariz Saulo Rios , Figueiredo Tânia Maria Ribeiro Monteiro de , Fook Sayonara Maria Lia
Published: December 2021

Trata-se de um estudo observacional, analítico e transversal, de caráter quantitativo, realizado a partir de dados secundários provenientes do Sistema de Informação de Agravos de Notificação (SINAN). Objetivou-se analisar o padrão espacial dos casos de tentativas de suicídio por medicamentos no município de Campina Grande/PB, em 2019. Foi realizado estatística descritiva, assim como elaboração de mapas (Moran e Lisa) para a verificação da distribuição espacial dos casos em estudo. Realizou-se, também, mapa coroplético para demonstrar a ocorrência e a intensidade do fenômeno e Diagrama de Espalhamento de Moran, utilizando-se o Índice de Moran Global e o Índice de Moran Local, com auxílio do software estatístico gratuito The R Project for Statistical Computing. Para nortear a análise espacial, utilizou-se a variável “bairro”. A amostra totalizou 237 pacientes residentes na zona urbana de Campina Grande que tentaram suicídio através do uso de medicamentos. O perfil dos pacientes obedeceu o seguinte padrão: sexo feminino (79,7%), faixa etária de 20 a 39 anos (51,5%), escolaridade em nível fundamental (49,4%), solteiro (a) como estado civil (64,5%) e a maioria sendo estudante (42,2%), seguido de desempregado (a) (32,1%). Os bairros que apresentaram o maior número de casos foram: Malvinas (n=23), Bodocongó (n=21) e Serrotão (n=9). Por outro lado, os bairros Mirante, Cuités, Sandra Cavalcante, Louzeiro, Itararé e Velame não apresentaram ocorrências de casos de tentativas de suicídio através do uso de medicamentos. Assim, verificou-se que, no quesito global, não houve autocorrelação espacial significativa na região estudada, com Índice de Moran Global encontrado de 0,1210.
Associação de níveis séricos de vitamina D3 e doenças orais – Revisão Sistemática
Authors: Santana Stephane Silva , Silva Ivânia Aparecida Pimenta Santos , Santos Gustavo Augusto , Almeida Adriano Luís Pulquério de , Andrade Rodrigo Soares de
Published: December 2021

O metabólito ativo da vitamina D3, o colecalciferol, é um hormônio multifuncional, que atua na regulação da homeostase do cálcio e do fósforo e que além disso, vem mostrado resultados promissores como imunomodulador, agente antitumoral entre outras funções. Destarde, essa revisão sistemática objetiva revisar na literatura científica como o colecalciferol atua como modulador hormonal e qual é seu papel frente a patologias orais. Foi realizada uma busca nas bases de dados PubMed (MEDLINE), Scopus, Embase, Google Scholar e The Cochrane Library, tendo como critérios de inclusão estudos os quais recorrerão assuntos referente à saúde oral associada ao uso do colecalciferol. Encontramos várias patologias orais que fazem associação com o colecalciferol, e verificamos seu papel na inflamação, na quimioprevenção e como um agente antitumoral, revelamos também que níveis baixos dessa vitamina estão associados ao aparecimento e /ou predisposição de patologias orais. Concluímos que a manutenção de níveis séricos ideais de colecalciferol é muito importante para a preservação e manutenção saúde bucal assim como para a saúde sistêmica.
O processo coletivo estrutural como forma de remediar a normatividade simbólica da educação ambiental
Authors: Barros Ana Luiza Rocha , Santos Larissa Martins Alves dos , Gomes Magno Federici
Published: December 2021

A falta de consciência e formação crítica ambiental é um desafio mundial. O presente artigo objetiva estudar o processo estrutural de interesse público como forma de implementar uma efetiva educação ambiental, ante o poder e dever de atuação Estatal e social do meio ambiente. Para abordagem, utilizar-se-á o método hipotético-dedutivo, a pesquisa qualitativa exploratória baseada em levantamento bibliográfico e documental. Verifica-se como resultado que o processo estrutural de interesse público pode ser instrumento hábil à efetivação da educação ambiental, ao instar o Poder Público, por meio de medidas estruturantes, a inseri-la como disciplina obrigatória no ensino básico brasileiro.
Avaliação da eficácia dos antiparasitários em ovinos da Fazenda Experimental Unama, Santarém-PA
Authors: Santos Amanda Cristiane Ferreira dos , Alves Monik de Lima , Reis Alessandra dos Santos Belo
Published: December 2021

O presente trabalho objetivou avaliar a eficácia dos antiparasitários Albendazol e Levamizol em ovinos naturalmente infectados na Fazenda Experimental da Unama. Foram utilizados 22 animais, divididos em três grupos: quatro animais controle (não tratado); nove tratados com Albendazol; e nove tratados com Levamizol. Realizou-se avaliação pré-tratamento e pós-tratamento (0º, 14º dia) e, após contagem de ovos por grama de fezes, foi calculado a porcentagem de redução (RCOF). Observou-se que ovinos tratados com Albendazol obteve baixa efetividade no 14º dia alcançando RCOF de 10%. Já no grupo tratado com Levamizol, teve RCOF de 70% no 14º dia. Conclui-se que os antiparasitários não foram eficazes no tratamento, demonstrando que outras medidas devem ser incluidas no manejo dos animais, além da avaliação periódica através do exame de fezes para verificar a eficácia dos medicamentos.
Avaliação do tempo de permanência como um indicador de qualidade na assistência ao paciente do serviço de urgência e emergência: uma revisão de literatura
Authors: Barroso Francisca Luana Vieira , Rocha Josivandra Luiane Pimenta da , Cavalcante Francisca Farias
Published: December 2021

Os protocolos de atendimento ao usuário, norteados pelos princípios da Política de Humanização (PNH), trouxeram aos gestores e usuários uma nova concepção de assistência no SUS, a satisfação do usuário sobre aos cuidados prestados, foi considerado um indicador de qualidade. Então todos os serviços de saúde passaram a trabalhar com a PNH, contemplando também os serviços de urgência e emergência. Discorrer sobre a avaliação do tempo de espera como indicador de qualidade do serviço de urgência, abordar implantadas, correlacionar os achados com a realidade do serviço apresentado nas literaturas. Caráter descritivo, revisão de literatura, utilizando artigos de periódicos científicos da área da saúde, teses e dissertações, disponíveis em: SCIELO, LILACS, MEDLINE; nos idiomas português, inglês e espanhol. Analisados pelo método de Bardin. Na atenção de urgência e emergência, pronto atendimento, assistência pré-hospitalar e outros; as diretrizes a serem seguidas são: acolher a demanda pela avaliação de risco, garantir o acesso referenciado, aumentar a resolutividade, prover o acesso à estrutura hospitalar e a transferência segura, caso seja necessário. O tempo de espera em casos de emergência é crucial para o desfecho do paciente e não pode ser protelado, podendo ser mitigado através da aplicação de protocolos organizativos. Destaca -se também que todas as fases pro processo de ACCR devem ser seguidas, pois falhas em um ponto do processo, podem acarretas em desfechos como superlotação da unidade, agravamento de quadros clínicos dos pacientes ainda em sala de espera e sobrecarga de profissionais.
Analisis Wacana Kritis Keputusan Pembubaran Front Pembela Islam di Kompas.com
Authors:   Universitas Budi Luhur, Jakarta Indonesia, Suryawati Indah , Jamalullail Jamalullail ,   Universitas Budi Luhur, Jakarta Indonesia
Published: December 2021

The government's decision regarding the dissolution and termination of all FPI activities raises pro and contra among the media and the public. The dissolution of FPI was decided by means of a decree signed by six ministers and heads of state institutions. This study aims to describe and dismantle the discourse of the news text on the decision to dissolve FPI using Critical Discourse Analysis (CDA). The research method used is Norman Fairclough's Critical Discourse Analysis through understanding text and context. There are five steps that are used as a reference. First, focus on social irregularities in its semiotic aspects. Second, identify barriers to address the social irregularities. Third, consider whether social order requires social irregularity. Fourth, identify possible ways to overcome these obstacles. Fifth, reflect critically from those four points. The results showed that kompas.com put FPI as the wrong position and the government's decision to dissolve FPI through an SKB was right. It is clear that kompas.com sided with the government. Kompas.com follows part of the flow of public votes in its news about FPI as a business strategy. The positioning of kompas.com was carried out because the government's decision to dissolve FPI caused pro and contra among the media and the public.
Nové zdroje daňových príjmov a definičný problém zdaňovania inteligentných systémov
Authors:   Pavol Jozef Šafárik University in Košice, Bujňáková Mária , Sábo Jozef ,   Pavol Jozef Šafárik University in Košice
Published: December 2021

Tento príspevok pozostáva z dvoch častí. V prvej časti autori venujú pozornosť stavu verejných financií po pandémii Corona-19 na Slovensku. Stav verejných financií analyzujú autori v kontexte vládnych iniciatív pre zavedenie nových politík znamenajúcich výdavky pre štátny rozpočet. Autori hodnotia tieto iniciatívy kriticky. V druhej časti príspevku navrhujú autori zavedenie dane z AI a robotov (označovanej v príspevku aj „AI/R daň“). V ďalšom texte príspevok poukazuje na jeden z hlavných problémov prijatia takýchto nových daňových inštrumentov zameraných na zdanenie AI a robotov a to na problém legálneho takéhoto predmetu zdanenia.
Transferové oceňovanie a COVID 19
Authors:   Univerzita Komenského, Bratislava, Choma Andrej , Rakovský Peter ,   Univerzita Komenského, Bratislava
Published: December 2021

Usmernenie OECD pre oblasť transferového oceňovania v čase pandémie COVID 19 bolo očakávané mnohými nadnárodnými spoločnosťami, ktorých účtovné závierky mali byť uzavreté do konca roka 2020. Nadnárodné spoločnosti museli zohľadniť finančné dopady pandémie COVID 19 aj v nastavení transferových cien pre rok 2020. Vzhľadom na rýchlosť vydania usmernenia je nutné OECD chváliť, ale aj s ohľadom na náročnosť dosahovania zásadných dohôd členov na úrovni OECD, neočakávali sa zásadné úpravy čo do obsahu samotného usmernenia.
Miestne dane a miestne poplatky v kontexte opatrení prijatých v súvislosti s chorobou COVID-19
Authors: Kubincová Tatiana
Published: December 2021

Pandémia koronavírusu sa dotkla každého z nás. Má globálne spoločenské a ekonomické dopady. V záujme zmiernenia následkov pandémie v Slovenskej republike došlo k prijatiu niekoľkých právnych predpisov, ktorých cieľom bola ochrana, resp. pomoc fyzickým osobám - nepodnikateľom, podnikateľom a zamestnancom aj zamestnávateľom, pred následkami pandémie. Príspevok poukazuje na právnu úpravu opatrení prijatých v oblasti miestnych daní a miestnych poplatkov.
Zlaté mince a oslobodenie od DPH - je všetko investičná minca čo sa blyští?
Authors: Poništ Viliam
Published: December 2021

Príspevok sa zameriava na režim dane z pridanej hodnoty v prípade zlatých investičných mincí. Opisuje systém EÚ oslobodenia od dane z pridanej hodnoty v prípade investičných zlatých mincí. Rozoberá niektoré príklady investičných mincí zo Slovenska, Česka, Československa a Rakúska, na ktorých sa snaží poukázať na nie celkom ideálne nastavený systém oslobodenia od DPH. Rieši jednotlivé príklady zlatých mincí a otázku ich oslobodenia alebo neoslobodenia od DPH.
Finančnoprávne aspekty kryptoaktív
Authors:   Pavol Jozef Šafárik University in Košice, Putera Marko
Published: December 2021

Cieľom tohto príspevku je analyzovať a charakterizovať jednotlivé druhy kryptoaktív, s poukazom najmä na zásadné rozdiely v spôsobe ich využitia, a následne aplikovať na tieto druhy pojmové znaky mien, peňazí a platidiel v kontexte základných zákonitostí finančného práva. Dôležitým teda bude rozlíšenie kryptoaktív podľa účelu ich používania, tzv. use case, pričom toto rozdelenie bude signifikantné pri vyhodnocovaní splnenia jednotlivých pojmových znakov mien, peňazí a platidiel. Základným cieľom výskumu bude zodpovedanie otázky, ako možno kryptoaktíva právne charakterizovať podľa súčasných všeobecne platných a uznávaných pravidiel a zákonitostí finančného práva.
Stroke in Patients with Diabetes: Is It Time to Expand Public Health Priority to Encompass High-Risk Patients with Increased Insulin Resistance?
Authors: Bradley Sian Alexandra , Varghese Francis Muttamthottil , Menon Bindu , Mehndiratta Man Mohan , Bhaskar Sonu Menachem Maimonides
Published: December 2021

Diabetes and stroke, with an interlinking aetiology, contribute to a growing cardiovascular disease burden and mortality around the world. Given the disproportionate prevalence and the burden of these conditions in the developing world, as well as the high risk of both Type 2 diabetes and cardiovascular disease carried by patients with metabolic syndrome, public health strategies are vital to mitigate the impact. Systematic approaches towards identifying undiagnosed patients in the community and building health systems around those targeted interventions have been implemented. However, growing evidence indicates potential for approaches to capture high-risk patients, such as those who suffer from pre-diabetes or increased insulin resistance, to provide early and optimal treatments, which could translate to population-level benefits, including reduced prevalence, disability, and disease burden.
Use Of Alternative Aggregates In Pavement Concrete: Research And Practice In Belgium
Authors: Boonen Elia , Smets Slyvie , Van der Wielen Audrey
Published: December 2021

Alternative aggregates, including recycled (concrete) aggregates as well as artificial aggregates (such as crushed stainless steel slag), are being increasingly used in road construction in the context of a more circular economy, e.g. in base and subbase layers. As these materials are applied higher up in the structure (surface and binder courses), stricter requirements are made to allow for higher loads and the stronger influence of the environment, and the application of these aggregates becomes less straightforward. The Belgian standard specifications, for instance, allow incorporation of recycled concrete aggregates in concrete pavements or linear elements in concrete, but only if certain stringent requirements are met and only up to certain percentages of substitution of the natural coarse aggregates. Furthermore, artificial aggregates originating from stainless steel slag are not even allowed for the time being in pavement quality concrete, although a Belgian standardization working group has recently been installed to investigate this matter in more detail. This paper presents an overview of laboratory research conducted in Belgium to characterize several types of alternative aggregates and concrete mixtures incorporating them, while focusing on practical execution as well as on the durability of pavement concrete. In addition, several concomitant pilot applications in Belgium are discussed in view of future perspectives for the application of alternative (recycled and/or artificial) aggregates in road construction.
Arquivo, memória e Big Data
Authors: Marino Ian Kisil , Telles da Silveira Pedro , Lima Nicodemo Thiago
Published: December 2021

Recebido: 20/07/2020

Aprovado: 25/07/2020

 

Este artigo busca refletir sobre os impactos das tecnologias digitais nas formas de arquivamento contemporâneo e apresentar a perspectiva de atuação elaborada no âmbito do projeto Memória Covid-19 Brasil, através do trabalho conjunto dos integrantes do Centro de Humanidades Digitais IFCH-UNICAMP e do projeto DéjàVu, da mesma instituição. Primeiro, abordaremos o caráter da memória na contemporaneidade, as tecnologias digitais e o deslocamento do lugar do historiador, principalmente tendo em vista o desafio apresentado pela pandemia do novo coronavírus. Depois, faremos um breve levantamento das iniciativas de arquivamento e constituição da memória da Covid-19 no país, para, em seguida, ressaltar as especificidades da proposta do Centro de Humanidades Digitais IFCH-UNICAMP, incluindo a criação de ferramentas informatizadas para a coleta de publicações. Por fim, retomaremos a importância da adoção de uma postura mais ativa pelos historiadores diante dos problemas contemporâneos, o que implica, também, repensar a constituição das nossas equipes de pesquisa e o aprofundamento da interdisciplinaridade de nossa atuação.

Palavras-chave: História Digital; Memória; Covid-19; Arquivos Digitais; Teoria da História.
A Autoria Feminina na Revista do Instituto Histórico e Geográfico de Sergipe (1939-2019)
Authors: De Souza Lopes Marluce , Tavares da Conceição Joaquim
Published: December 2021

Recebido: 29/04/2020

Aprovado: 01/06/2020

Este artigo tem como objetivo catalogar a produção feminina na Revista do Instituto Histórico e Geográfico de Sergipe, no período compreendido entre 1939 e 2019, ressaltando a escrita da mulher, bem como as temáticas nas quais a produção está inserida, destacando sua participação como escritora e produtora de cultura. Foram consultadas as edições digitalizadas da Revista do Instituto Histórico e Geográfico de Sergipe, disponíveis no site do Instituto Histórico e Geográfico de Sergipe. A supremacia masculina na revista foi mantida até 1939, quando surgiram as primeiras colaborações femininas, no entanto, foi a partir dos anos 1970 que esse cenário começou a ser alterado, com a crescente participação das mulheres na publicação de gêneros diversificados, suplantando, em algumas edições, o número de trabalhos masculinos. Foram encontrados 207 textos de autoria feminina, com destaque para Maria Thétis Nunes e Beatriz Góis Dantas, as intelectuais que mais publicaram no periódico analisado.

 Palavras-chave: Autoria feminina, Intelectuais, Revista do Instituto Histórico e Geográfico de Sergipe.
Pesquisas em História:
Authors: De Oliveira Simas Alicy
Published: December 2021

Recebida: 24/06/2020 

Aprovada: 30/10/2020
HYPOTHESES TESTING ON THE VALIDITY OF EFFECTIVE COMMUNICATION AND COMMON MOTIF OF SELAYAH KERINGKAM
Authors: Mansor Norsyahbany , Abdullah Qistina Donna Lee
Published: December 2021

This paper is to deliberate the subjects of the effective communication channel in delivering common motifs in Selayah Keringkam by assessing the local and International tourists’ preference. It is to evaluate the hypotheses Testing On validity of Effective Communication and Common Motif of Selayah Keringkam Towards the communication channel. The finding of this paper is to enhance the implications on developing a significant way to disseminate Selayah Keringkam as a heritage product in Sarawak. This paper will expose a substantial relationship between the independent variables (Common Motif of Selayah Keringkam), the dependent variables (Channels of Communication) and the mediator (Supplement of Effective Communication) as a new framework to the body of knowledge. This research involved 384 respondents with 5 relevant authorities related to answering research questions. The data collected from the fieldwork were analysed using SPSS version 24. The measures used in this article are methodically deliberated using factor analysis and correlation analysis. Lastly, the results of the regression analyses testing on hypotheses and summary of hypotheses assessments are presented.
THE STUDY OF MATERIAL CULTURE OF THE IBAN COMMUNITY IN SARAWAK: NGEPAN
Authors: Kiyai Gregory , Anak Tugang Noria
Published: December 2021

Cultural identity is a specific word that refers to individuals and communities in distinguishing themselves from others. One of the most dominant identities in a community culture is traditional clothing. In Sarawak, the traditional dress for the Iban is commonly known as Ngepan Indu. The Ngepan Indu is often worn during the Gawai Dayak celebration season as well as several other ritual ceremonies such as the Miring ceremony (Offerings) and Melah Pinang (Marriage). The Iban community believes that the Ngepan Iban is an identity and cultural icon in today's customs. Apart from that, Ngepan Iban is one of the treasures and legacies of Petara (God) who inhabited a kingdom called Panggau Libau, Tinting Gawai Gasing Gerah Layang so that Iban always remembered Petara and the origin of Iban's ancestors. This paper aims to analyze and understand the Ngepan Indu in the context of the custom and culture of the Iban community by using the qualitative research method based on the collective memory of the Iban cultural experts in Sarawak. Through this research, it is expected to have an effective and progressive impact to regenerate the spirit of Iban culture to continue persistently preserve the Ngepan Iban as a cultural heritage from the ancestors of the past, so that it will continue to be preserved for the future.
THE DUSUN TINDAL RESIDENTS OF LASAU TINTAPON IN KOTA BELUD, SABAH, A TRADITION OF USING ARTEFACT TAJAU
Authors: Andy Maxwilliam , Tugang Noria , Banaas Benigel Royce , Bin Natus Alvine
Published: December 2021

This research looked into the use of artefact tajau as one of the Dusun Tindal counting traditions. In addition, this report will clarify how this native used the term tajau a century ago. This research also employs a qualitative approach fieldwork method with sources being interviewed to gather evidence. The researcherwas taken to the study site by an informant who is an expert and a traditional chief in the village. This approach is employed so that the researcher can analyze and comprehend the study’s use of the tajau in greater detail. Futhermore, this research demonstrates that tajau has been used since the first war until thepresent century.
A Normative Survey on Auditory Perception and Semantic Descriptors of Musical Timbres
Authors: Anis Haron  , Wong Chee Onn  , Hew Soon Hin
Published: December 2021

Timbre are commonly described using semantic descriptors such as ’dark’, ’bright’ and ’warm’. The use of such descriptors are useful and largely practiced by trained individuals in music related industries. Such descriptors are subjective as it could be interpreted differently by different individuals determined by factors such as training and exposure. Semantic descriptors also lacks granularity, in a sense that the descriptor does not indicate the amount or intensity of the description. A numerical representation for timbral description addressees these issues. Computational approach for numerical measure of timbre are at present under study by music technology researchers. Such studies requires a benchmarking process in order for viability tests. To provide a set of data that can be used for benchmarking, a survey on auditory perception and semantic descriptors of musical timbres were conducted. The conducted survey looks to find out if a general consensus can be observed for semantic description of musical timbres using a normative survey methodology. This article reviews the conducted survey, presenting the survey’s approach, results and findings
The Implementation of Strategic Threat Intelligence for Business Organization
Authors: Leong Yee Ling
Published: December 2021

Nowadays strategic threat intelligence is very important to all the organization. Strategic cyber threat intelligence can determine who and why to provide key insights to the organization. It purpose is to determine who is behind a particular threat or threat family and addressing to evolving trends. The strategic level of cyber threat intelligence also included and explains about why. Why makes a company or an organization a target? Strategic Threat Intelligence offer the overview of the threat status of the organization. Therefore, the C-Suite include chief executive officer (CEO), chief financial officer (CFO), chief operating officer (COO) and chief information officer (CIO) of the organization use cyber threat intelligence data to understand the high-level trends and threats to the company or the organization. The C-Suite of the organization also need to know how to implement the strategic threat intelligence to prevent unexpected things happen. This research paper aims to discuss about the importance of the strategic threat intelligence to the company or organization and how to implement it. After knowing and understanding the implementation of strategic threat intelligence to the company or organization, this research paper also will discuss about the when of using strategic threat intelligence. The issue and challenges is also discussed in the article.
Authentication of IoT device with the enhancement of One-time Password (OTP)
Authors: Chan Suet Yan Sharon , Tang Su Wei Alice , Bong Jie Hui , Teh Quor Ling , Sivalingam Shanmugapiriya , Khoo Shi Yee , Nafy Tahmid Mutashim
Published: December 2021

The Robust and Energy Efficient Authentication Protocol works for Industrial Internet of Things. The Internet of Things (IoT) is an arising innovation and expected to give answers for different modern fields. The IoT enable connection of physical devices all around the world to the internet by collecting and sharing critical and real-time data among each other. The increment of devices increases the computational cost during data transmission between devices and towards the internet. In this paper we proposed a solution that is a multi-factor authentication protocol to enhance the protocol proposed by Li et al. For Industrial IoT by adding One Time Password (OTP) after the biometric information of the user is checked by the Gateway Node (GWN) to be able to tackle additional network attack aside from those that are overcome by Li et al. scheme. Our contribution for this project is, we proposed the solution that a multi-factor authentication protocol to enhance the protocol proposed. For Industrial IoT by adding One Time Password (OTP) after the biometric information of the user is checked by the Gateway Node (GWN) to be able to tackle additional network attack aside from those that are overcome. The idea of adding OTP is inspired by where they scheme correlates to biometric of user as well. Our proposal is lower cost than the three protocols regarding authentication overhead and computational cost perspectives. Challenges and future directions of this paper examined the security shortcomings of a client confirmation convention for WSN, which is as proposed by Chang and Le. To address the normal security shortcomings of past protocols, we proposed a strong and energy effective three-factor authentication protocol for WSN.
Features of tax control of the new EU countries: the example of Latvia
Authors:   University of the Fiscal Service of Ukraine, LYSETSKA Nelya
Published: December 2021

The paper substantiates comprehensive approaches to the formation of an effective mechanism for tax control of income and expenses of taxpayers in the Baltic States, in particular in Latvia. In order to effectively implement methods of controlling income and expenditure on a legal basis, first of all, it is necessary to start comprehensive and persistent fight against shadow income. Methodological approaches should include: attracting public investment to create an information digital infrastructure for processing declarations; the elimination of shadow flows, as well as the implementation of universal declaration using indirect methods of determining tax liabilities and the general modernization of the tax system. The anti-corruption strategy of modern state legislation should be aimed not only at the inevitability of punishment for corrupt practices, but also at the consistent elimination of the conditions for corruption.
Connecting Europe Facility in the system of joint financing of the EU critical infrastructure
Authors:   SESE “The Academy of Financial Management”, BALAKIN Robert
Published: December 2021

The study shows components of financial support for the formation and development of critical infrastructure entities in the European Union. The sustainable functioning of critical infrastructure is aimed at the observance of common Union interests, given the existence of differences in the economic policy in different EU Member States. It was found that the development of Trans-European infrastructure programs is carried out within the framework of the EU Cohesion Policy. The Connecting Europe Facility is a key special tool for funding critical infrastructure in the EU transport, energy and digital services sectors. The Fund is mainly used to finance entities aimed at achieving the goals of the European Green Deal. Recommendations for Ukraine to take into account the experience of financial support regulation for the development of the EU critical infrastructure are substantiated. Based on the experience of the EU, the criteria for determining the priority of the project for financing critical infrastructure are highlighted. Based on the analysis of the formation and use of the Connecting Europe Facility as the main common instrument for financing the EU critical infrastructure, a conclusion was made on the feasibility of establishing a critical infrastructure development fund of Ukraine to support financing of key projects in transport, digital and energy infrastructure. Special rules for determining the eligible costs incurred for financing infrastructure projects at the expense of the critical infrastructure development fund of Ukraine are disclosed.
Nutrix - Artificial Intelligence for Nutritionists
Authors: Crespo Alvarez Jorge , Ferreira Hernández Bryan , Sumalla Cano Sandra
Published: December 2021

This work, developed under the NUTRIX Project, has the objective to develop artificial intelligence algorithms based on the open source platform Knime that allows to characterize and predict the adherence of individuals to diet before starting the treatment. The machine learning algorithms developed under this project have significantly increased the confidence (a priory probability) that a patient leaves the treatment (diet) before starting: from 17,6% up to 96,5% which can be used as valuable guidance during the decision-making process of professionals in the area of ​dietetics and nutrition.
Laser Enrichment Device Stimulates Broiler Laser-Following Behavior While Increasing Individual Bird Locomotion and Pen-Wide Movement
Authors: Meyer Meaghan M. , Johnson Anna K. , Bobeck Elizabeth A.
Published: December 2021

Genetic selection for fast growth rate and high breast muscle yield in modern broilers has unintended effects on animal welfare and behavior, namely in terms of inactivity and leg disorders. We hypothesized that exercise stimulated through environmental enrichment could positively stimulate pen-wide activity and improve bird welfare. The study objectives were to implement a laser enrichment device to motivate active and feeding behaviors throughout the pen. Twelve hundred Ross 708 broilers were randomly assigned to enrichment (LASER; laser enrichment, or CON; no laser enrichment) for 49 d. Seventy focal birds were randomly assigned to 14 video-recorded pens for behavioral analysis, including focal bird home pen behavior and walking distance. Pen-wide activity was also measured during the 4-min laser periods, four times daily, d0–8, and 1 day weekly, wk 1–6. Focal birds were gait scored wk 1–6, and were euthanized on d42 for tibia bone mineral content, density, and bone breaking strength analysis. Time spent active was increased in LASER-enriched birds compared to CON on wk 3–5 by up to 214% (wk 4), and percent of time at the feeder was increased in LASER-enriched birds by 761% on wk 4 (P< 0.05). Peak percent of birds following the laser (LASER-enriched pens only) was observed on d0 (8.52%). Over wk 1–6, peak laser-following behavior was observed on wk 3 (3.07% of birds). Percent of birds moving during laser periods was increased in LASER-enriched pens on d0, 1, 2, 6, 7, and 8, with a percent increase of 68.7% observed on d1 (P< 0.05). Percent of birds moving (laser-following or not) was increased on wk 1, 3, and 4 in LASER-enriched pens, with an increase of 69.7% observed on wk 4 (P< 0.05). No differences were found in tibia measures. These data indicate that laser enrichment stimulated voluntary locomotion through wk 5 and laser-following behavior through wk 6, and that the relatively small percent of birds actively following the laser stimulated pen-wide movement above the level of the CON through wk 4 on study.
Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
Authors: Semenova Ekaterina , Grudniak Mariusz P. , Bocian Katarzyna , Chroscinska-Krawczyk Magdalena , Trochonowicz Marzena , Stepaniec Igor M. , Murzyn Magdalena , Szablowska-Gadomska Ilona , Boruczkowski Dariusz , Oldak Tomasz , Machaj Eugeniusz K.
Published: December 2021

Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential.
Paper-Based Point-of-Care Testing of SARS-CoV-2
Authors: Jia Yuan , Sun Hao , Tian Jinpeng , Song Qiuming , Zhang Wenwei
Published: December 2021

The COVID-19 pandemic has resulted in significant global social and economic disruption. The highly transmissive nature of the disease makes rapid and reliable detection critically important. Point-of-care (POC) tests involve performing diagnostic tests outside of a laboratory that produce a rapid and reliable result. It therefore allows the diagnostics of diseases at or near the patient site. Paper-based POC tests have been gaining interest in recent years as they allow rapid, low-cost detection without the need for external instruments. In this review, we focus on the development of paper-based POC devices for the detection of SARS-CoV-2. The review first introduces the principles of detection methods that are available to paper-based devices. It then summarizes the state-of-the-art paper devices and their analytical performances. The advantages and drawbacks among methods are also discussed. Finally, limitations of the existing devices are discussed, and prospects are given with the hope to identify research opportunities and directions in the field. We hope this review will be helpful for researchers to develop a clinically useful and economically efficient paper-based platform that can be used for rapid, accurate on-site diagnosis to aid in identifying acute infections and eventually contain the COVID-19 pandemic.
Application of Programmable Tetrahedral Framework Nucleic Acid-Based Nanomaterials in Neurological Disorders: Progress and Prospects
Authors: Chen Xingyu , Xie Yu , Liu Zhiqiang , Lin Yunfeng
Published: December 2021

Tetrahedral framework nucleic acid (tFNA), a special DNA nanodevice, is widely applied in diverse biomedical fields. Due to its high programmability, biocompatibility, tissue permeability as well as its capacity for cell proliferation and differentiation, tFNA presents a powerful tool that could overcome potential barriers in the treatment of neurological disorders. This review evaluates recent studies on the use and progress of tFNA-based nanomaterials in neurological disorders.
Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
Authors: Lv Xianqing , Yang Guangxu , Gong Zhenggang , Cheng Xin , Shuai Li , Huang Liulian , Chen Lihui , Luo Xiaolin , Liu Jing
Published: December 2021

Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessibility that was 10 times that of untreated bamboo. However, deposited lignin could trigger non-productive adsorption to enzymes, which therefore significantly decreased the enzymatic hydrolysis efficiency of PSA-pretreated bamboo substrates. Herein, poly(N-vinylcaprolactam) (PNVCL), a non-ionic surfactant, was developed as a novel additive for overcoming the non-productive adsorption of lignin during enzymatic hydrolysis. PNVCL was found to be not only more effective than those of commonly used lignosulfonate and polyvinyl alcohol for overcoming the negative effect of lignin, but also comparable to the robust Tween 20 and bovine serum albumin additives. A PNVCL loading at 1.2 g/L during enzymatic hydrolysis of PSA pretreated bamboo substrate could achieve an 80% cellulosic enzymatic conversion and meanwhile reduce the cellulase loading by three times as compared to that without additive. Mechanistic investigations indicated that PNVCL could block lignin residues through hydrophobic interactions and the resultant PNVCL coating resisted the adsorption of cellulase via electrostatic repulsion and/or hydration. This practical method can improve the lignocellulosic enzymatic hydrolysis efficiency and thereby increase the productivity and profitability of biorefinery.
Elastin Structure, Synthesis, Regulatory Mechanism and Relationship With Cardiovascular Diseases
Authors: Wang Keke , Meng Xiangguang , Guo Zhikun
Published: December 2021

As the primary component of elastic fibers, elastin plays an important role in maintaining the elasticity and tensile ability of cardiovascular, pulmonary and many other tissues and organs. Studies have shown that elastin expression is regulated by a variety of molecules that have positive and negative regulatory effects. However, the specific mechanism is unclear. Moreover, elastin is reportedly involved in the development and progression of many cardiovascular diseases through changes in its expression and structural modifications once deposited in the extracellular matrix. This review article summarizes the role of elastin in myocardial ischemia-reperfusion, atherosclerosis, and atrial fibrillation, with emphasis on the potential molecular regulatory mechanisms.
α4/α9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways
Authors: Hight-Warburton Willow , Felix Robert , Burton Andrew , Maple Hannah , Chegkazi Magda S. , Steiner Roberto A. , McGrath John A. , Parsons Maddy
Published: December 2021

Adhesion of basal keratinocytes to the underlying extracellular matrix (ECM) plays a key role in the control of skin homeostasis and response to injury. Integrin receptors indirectly link the ECM to the cell cytoskeleton through large protein complexes called focal adhesions (FA). FA also function as intracellular biochemical signaling platforms to enable cells to respond to changing extracellular cues. The α4β1 and α9β1 integrins are both expressed in basal keratinocytes, share some common ECM ligands, and have been shown to promote wound healingin vitroandin vivo.However, their roles in maintaining epidermal homeostasis and relative contributions to pathological processes in the skin remain unclear. We found that α4β1 and α9β1 occupied distinct regions in monolayers of a basal keratinocyte cell line (NEB-1). During collective cell migration (CCM), α4 and α9 integrins co-localized along the leading edge. Pharmacological inhibition of α4β1 and α9β1 integrins increased keratinocyte proliferation and induced a dramatic change in cytoskeletal remodeling and FA rearrangement, detrimentally affecting CCM. Further analysis revealed that α4β1/α9β1 integrins suppress extracellular signal-regulated kinase (ERK1/2) activity to control migration through the regulation of downstream kinases including Mitogen and Stress Activated Kinase 1 (MSK1). This work demonstrates the roles of α4β1 and α9β1 in regulating migration in response to damage cues.
Development of an Oxidative Phosphorylation-Related and Immune Microenvironment Prognostic Signature in Uterine Corpus Endometrial Carcinoma
Authors: Liu Jinhui , Chen Tian , Yang Min , Zhong Zihang , Ni Senmiao , Yang Sheng , Shao Fang , Cai Lixin , Bai Jianling , Yu Hao
Published: December 2021

Background: As the fourth most common malignant tumors in women, uterine corpus endometrial carcinoma (UCEC) requires novel and reliable biomarkers for prognosis prediction to improve the overall survival. Oxidative phosphorylation (OXPHOS) is found to be strongly correlated with the progression of tumor. Here, we aimed to construct an OXPHOS-related and immune microenvironment prognostic signature to stratify UCEC patients for optimization of treatment strategies.

Method: Prognosis-associated OXPHOS-related differentially expressed genes were identified by multivariable Cox regression from TCGA–UCEC cohort. Based on the candidate genes, an OXPHOS-related prognostic signature was constructed by the train set data and verified by the entire set. When integrated with relevant clinical characteristics, a nomogram was also created for clinical application. Through comparison of tumor microenvironment between different risk groups, the underlying mechanism of the model and the inner correlation between immune microenvironment and energy metabolism were further investigated.

Results: An OXPHOS-related signature containing ATP5IF1, COX6B1, FOXP3, and NDUFB11 was constructed and had better predictive ability compared with other recently published signatures in UCEC. Patients with lower risk score showed higher immune cell infiltration, higher ESTIMATE score (p = 2.808E−18), lower tumor purity (p = 2.808E−18), higher immunophenoscores (IPSs) (p < 0.05), lower expression of mismatch repair (MMR) proteins (p < 0.05), higher microsatellite instability (MSI), lower expression of markers of N6-methyladenosine (m6A) mRNA methylation regulators, higher tumor mutation burden (TMB) (p = 1.278E−9), and more sensitivity to immune checkpoint blockade (ICB) (p < 0.001) and chemotherapy drugs, thus, possessing improved prognosis.

Conclusion: An OXPHOS-related and immune microenvironment prognostic signature classifying EC patients into different risk subsets was constructed in our study, which could be used to predict the prognosis of patients and help to select a specific subset of patients who might benefit from immunotherapy and chemotherapy, thus, improving the overall survival rate of UCEC. These findings may contribute to the discovery of novel and robust biomarkers or target therapy in UCEC and give new insights into the molecular mechanism of tumorigenesis and progression of UCEC.
Insights Into Central Nervous System Glial Cell Formation and Function From Zebrafish
Authors: Neely Sarah A. , Lyons David A.
Published: December 2021

The term glia describes a heterogenous collection of distinct cell types that make up a large proportion of our nervous system. Although once considered the glue of the nervous system, the study of glial cells has evolved significantly in recent years, with a large body of literature now highlighting their complex and diverse roles in development and throughout life. This progress is due, in part, to advances in animal models in which the molecular and cellular mechanisms of glial cell development and function as well as neuron-glial cell interactions can be directly studied in vivo in real time, in intact neural circuits. In this review we highlight the instrumental role that zebrafish have played as a vertebrate model system for the study of glial cells, and discuss how the experimental advantages of the zebrafish lend themselves to investigate glial cell interactions and diversity. We focus in particular on recent studies that have provided insight into the formation and function of the major glial cell types in the central nervous system in zebrafish.
Comprehensive Analysis of Subtype-Specific Molecular Characteristics of Colon Cancer: Specific Genes, Driver Genes, Signaling Pathways, and Immunotherapy Responses
Authors: Hu Fangjie , Wang Jianyi , Zhang Minghui , Wang Shuoshuo , Zhao Lingyu , Yang Hao , Wu Jinrong , Cui Binbin
Published: December 2021

Colon cancer is a complex, heterogeneous disease. The Colorectal Cancer Subtyping Consortium reported a novel classification system for colon cancer in 2015 to better understand its heterogeneity. This molecular classification system divided colon cancer into four distinct consensus molecular subtypes (CMS 1, 2, 3, and 4). However, the characteristics of different colon cancer molecular subtypes have not been fully elucidated. This study comprehensively analyzed the molecular characteristics of varying colon cancer subtypes using multiple databases and algorithms, including The Cancer Genome Atlas (TCGA) database, DriverDBv3 database, CIBERSORT, and MCP-counter algorithms. We analyzed the alterations in the subtype-specific genes of different colon cancer subtypes, such as the RNA levels and DNA alterations, and showed that specific subtype-specific genes significantly affected prognosis. We also explored the changes in colon cancer driver genes and representative genes of 10 signaling pathways in different subtypes. We identified genes that were altered in specific subtypes. We further detected the infiltration of 22 immune cell types in four colon cancer subtypes and the infiltration level of primary immune cells among these subtypes. Additionally, we explored changes in immune checkpoint genes (ICGs) and immunotherapy responses among different colon cancer subtypes. This study may provide clues for the molecular mechanism of tumorigenesis and progression in colon cancer. It also offers potential biomarkers and targets for the clinical diagnosis and treatment of different colon cancer subtypes.
AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
Authors: Wang Shimin , Yao Longfeng , Zhang Wenjuan , Cheng Zihang , Hu Can , Liu Hang , Yan Yanling , Shi Anbing
Published: December 2021

Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia.