bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk factors for difficult peripheral venous cannulation in hospitalised patients. Protocol for a multicentre case–control study in 48 units of eight public hospitals in Spain
Authors: Rodriguez-Calero Miguel Angel , Fernandez-Fernandez Ismael , Molero-Ballester Luis Javier , Matamalas-Massanet Catalina , Moreno-Mejias Luis , de Pedro-Gomez Joan Ernest , Blanco-Mavillard Ian , Morales-Asencio Jose Miguel
Published: February 2018

Introduction

Patients with difficult venous access experience undesirable effects during healthcare, such as delayed diagnosis and initiation of treatment, stress and pain related to the technique and reduced satisfaction. This study aims to identify risk factors with which to model the appearance of difficulty in achieving peripheral venous puncture in hospital treatment.

Methods and analysis

Case–control study. We will include adult patients requiring peripheral venous cannulation in eight public hospitals, excluding those in emergency situations and women in childbirth or during puerperium. The nurse who performs the technique will record in an anonymised register variables related to the intervention. Subsequently, a researcher will extract the health variables from the patient’s medical history. Patients who present one of the following conditions will be assigned to the case group: two or more failed punctures, need for puncture support, need for central access after failure to achieve peripheral access, or decision to reject the technique. The control group will be obtained from records of patients who do not meet the above conditions. It has been stated a minimum sample size of 2070 patients, 207 cases and 1863 controls.

A descriptive analysis will be made of the distribution of the phenomenon. The variables hypothesised to be risk factors for the appearance of difficult venous cannulation will be studied using a logistic regression model.

Ethics and dissemination

The study was funded in January 2017 and obtained ethical approval from the Research Ethics Committee of the Balearic Islands. Informed consent will be obtained prior to data collection. Results will be published in a peer-reviewed scientific journal.
Long-term trends of liver cancer mortality by gender in urban and rural areas in China: an age-period-cohort analysis
Authors: Sun Yuanyuan , Wang Yanhong , Li Mengmeng , Cheng Kailiang , Zhao Xinyu , Zheng Yuan , Liu Yan , Lei Shaoyuan , Wang Li
Published: February 2018

Objectives

To provide an up-to-date overview of long-term trends of liver cancer mortality and evaluate the effects attributable to age, period and cohort in Chinese population stratified by gender and urban/rural areas.

Methods

Population and liver cancer mortality data were obtained based on the Disease Surveillance Points in China from 1991 to 2014. To examine the time trends of liver cancer mortality by gender in urban and rural areas in China, Joinpoint analysis was used to estimate the annual per cent change. The intrinsic estimator, a method of age-period-cohort analysis to estimate age, period and cohort effects simultaneously, was used to analyse the underlying mechanisms for liver cancer mortality trends in the aforementioned four groups.

Results

We observed a significant decline in liver cancer mortality for urban men (average annual per cent change (AAPC)=−1.1%, P<0.05) and urban women (AAPC=−1.4%, P<0.05), while the liver cancer mortality remained stable for rural men (AAPC=−0.1%, P>0.05) and rural women (AAPC=−0.9%, P>0.05). Compared with the 15–19 age group, the liver cancer mortality risk of the 85 and above age group increased 65 and 42 times for urban and rural men, and 102 and 70 times for urban and rural women. From the 1990–1994 period to the 2005–2009 period, the risk increased 56% and 92% for urban and rural men, and 30% and 74% for urban and rural women. Compared with period and cohort effects, age effects were the most influential factor in liver cancer mortality.

Conclusions

As the status of ageing population in China gets worse, the burden caused by liver cancer mortality could still be a great challenge for China in the future. The disparity of liver cancer mortality trends between urban and rural residents can be attributed to period effects, referring to the unequal medical levels and resources between urban and rural areas.
Telephone or integrated contraception counselling before abortion: impact on method choice and receipt
Authors: Lohr Patricia A , Aiken Abigail R A , Forsyth Tracey , Trussell James
Published: February 2018

Background

Incorporating thorough contraception counselling into an abortion consultation is challenging. We compared contraceptive choices and methods received between two counselling models: (1) telephone counselling separate from the abortion consultation and (2) face-to-face counselling integrated into the consultation.

Methods

We obtained de-identified data on demographic characteristics and contraceptive methods that had been chosen and received by women who had an abortion at British Pregnancy Advisory Service between 2011 and 2014 and had a choice of counselling models. We compared the characteristics of women who chose each model of counselling and the contraceptive methods they chose and received using Fisher’s exact test, and used logistic regression to explore associations between counselling model and choice and receipt of Tier 1 contraceptive methods (intrauterine contraception, implant, sterilisation), controlling for covariates.

Results

The sample included 18 573 women. Women choosing telephone counselling were more likely to be non-White (34% vs 22%, P<0.001), to report prior difficulty obtaining contraception (40% vs 3%, P<0.001), and to have not used contraception at conception (37.1% vs 33.8%, P<0.001). Overall, 93% of women chose a contraceptive method after counselling. Telephone counselling was significantly associated with both choosing and receiving a Tier 1 method (OR 1.80, 95% CI 1.66 to 1.96 and OR 1.60, 95% CI 1.42 to 1.71, respectively). Fewer women who had telephone counselling received a less effective method (eg, condom, diaphragm) compared with those who chose integrated counselling (6.0% vs 19.2%, P<0.001).

Discussion

Telephone-based contraception counselling separate from the abortion consultation may serve some women better than integrated counselling, particularly those reporting past difficulty obtaining contraception.
PD-1 and PD-L1 expression in thymic epithelial tumours and non-neoplastic thymus
Authors: Bagir Emine Kilic , Acikalin Arbil , Avci Alper , Gumurdulu Derya , Paydas Semra
Published: February 2018

Aims

We explored the relationships between programmed death-1 (PD-1) and programmed death-ligand 1 (PD-L1) expression and the pathological and clinical features of thymic epithelial tumours and thymic hyperplasia.

Methods

We evaluated PD-1 and PDL-1 expressions within epithelial and microenvironmental components in thymic epithelial tumours (n=44) and thymic hyperplasias (n=8), immunohistochemically. We compared the results with demographic, clinical and histopathological features of the cases.

Results

We found 48% epithelial expression and 82.7% microenvironment expression for PD-1 and 11.5% epithelial expression and 34.6% microenvironment expression for PD-L1. There was no PD-1 expression, in either the epithelial or microenvironment, in the thymic hyperplasia group. PD-1 and PD-L1 positivity was more significant in thymic epithelial tumours than thymic hyperplasia. Patients with PD-1-positive microenvironments exhibited significantly shorter mean estimated survival time than their negative counterparts.

Conclusion

These findings suggest that anti-PD-1 and anti-PD-L1 therapies may benefit patients due to high release of PD-1 and PD-L1 in thymic epithelial tumours.
High prevalence of gait abnormalities in pugs
Authors: Rohdin Cecilia , Jäderlund Karin Hultin , Ljungvall Ingrid , Lindblad‐Toh Kerstin , Häggström Jens
Published: February 2018

The objective of this prospective study was to determine the prevalence of gait abnormalities in a cohort of Swedish pugs by using an owner‐based questionnaire targeting signs of gait abnormality and video footage showing the dog's gait. This study also evaluated associated conditions of abnormal gait, including other health disorders prevalent in the breed. Five hundred and fifty (550) pugs registered in the Swedish Kennel Club, of one, five and eight years of age, in 2015 and 2016, were included in the study. Gait abnormalities were reported in 30.7 per cent of the responses. In the majority of cases, the character of the described gait indicated a neurological cause for the gait abnormality. An association was observed between abnormal gait and age, with gait abnormalities being significantly more common in older pugs (P=0.004). An association was also found between abnormal gait and dyspnoea, with dyspnoea being significantly more common in pugs with gait abnormalities (P<0.0001). This study demonstrated that the prevalence of gait abnormalities was high in the Swedish pug breed and increased with age. Future studies on the mechanisms behind these gait abnormalities are warranted.
Novel diaminopropyl substituted organotin compounds
Authors: Pichler Johann , Müller Philipp , Torvisco Ana , Uhlig Frank
Published: February 2018

A novel synthetic pathway involving the desilylation of a tin trimethylsilyl species (Ph2Sn(SiMe3)2) towards nonprotected di(3-aminopropyl)tin dichloride ((H2N(CH2)3)2SnCl2) is described. Di(3-aminopropyl)tin dichloride is then converted to the respective dicarboxylates species (H2N(CH2)3)2Sn(OCOR)2 containing carboxylic acids of different lengths (R = –CH3, –(CH2)10CH3). Depending on the nature of R, discrete packing effects are observed in the solid state of di(3-aminopropyl)tin dicarboxylate derivatives. All the synthesized substances were characterized by 1H, 13C, and 119Sn nuclear magnetic resonance data and also single crystal X-ray analysis. These compounds are a promising class of substances for biological, pharmaceutical, and technical applications.
SGE1 is involved in conidiation and pathogenicity of Fusarium oxysporum f.sp. cubense
Authors: Hou Xingrong , An Bang , Wang Qiannan , Guo Yunfeng , Luo Hongli , He Chaozu
Published: February 2018

The ascomycete fungus Fusarium oxysporum f.sp. cubense race 4 (Foc TR4) causes vascular wilt diseases in banana (Musa spp.). In the present study, the role of SGE1 in regulating growth, conidiation, and pathogenicity of Foc TR4 was investigated. Deletion of SGE1 did not influence vegetative growth but impaired the conidiation of Foc TR4. Besides, the SGE1 deletion mutant basically lost pathogenicity on banana plantlets. Observation under the microscope indicated that the penetration and colonization processes were severely impaired in the SGE1 deletion mutant. Proteomics analysis suggested that SGE1 regulated the production of a series of proteins of Foc TR4. Taken together, our results suggest that SGE1 plays an important role in regulating conidiation and pathogenicity in fungal pathogen Foc TR4.
The transition of a gravitationally radiating, dissipative fluid to equilibrium
Authors: Herrera L. , Di Prisco A. , Ospino J.
Published: February 2018

We describe the transition of a gravitationally radiating, axially and reflection symmetric, dissipative fluid to a non-radiating state. It is shown that very shortly after the end of the radiating regime, at a time scale on the order of the thermal relaxation time, the thermal adjustment time, or the hydrostatic time (whichever is larger), the system reaches the equilibrium state. This result is at variance with all the studies carried out in the past on gravitational radiation outside the source, which strongly suggest that after a radiating period, the conditions for a return to a static case look rather forbidding. As we shall see, the reason for such a discrepancy resides in the fact that some elementary, but essential, physical properties of the source have been overlooked in these latter studies.
Influence of an inclined magnetic field on the Poiseuille flow of immiscible micropolar–Newtonian fluids in a porous medium
Authors: Yadav Pramod Kumar , Jaiswal Sneha
Published: February 2018

The present problem is concerned with two-phase fluid flow through a horizontal porous channel in the presence of uniform inclined magnetic field. The micropolar fluid or Eringen fluid and Newtonian viscous fluid are flowing in the upper and lower regions of the horizontal porous channel, respectively. In this paper, the permeability of each region of the horizontal porous channel has been taken to be different. The effects of various physical parameters like angles of inclination of magnetic field, viscosity ratio, micropolarity parameter, etc., on the velocities, micro-rotational velocity of two immiscible fluids in horizontal porous channel, wall-shear stress, and flow rate have been discussed. The result obtained for immiscible micropolar–Newtonian fluids are compared with the results of two immiscible Newtonian fluids. The obtained result may be used in production of oil from oil reservoirs, purification of contaminated ground water, etc.
Identity-Based Batch Provable Data Possession with Detailed Analyses
Authors: Zhou Fucai , Peng Su , Xu Jian , Xu Zifeng
Published: February 2018

In the era of big data, cloud storages become prevalent while security issues on outsourced data emerge. Without the physical control of the data owners, the outsourced data faces more risks. Provable Data Possession (PDP) is a probabilistic technique for checking whether data is correctly stored in remote servers without retrieving the entire data. For many previous PDP schemes, correctly choosing public keys for data owners relies on the security of Public Key Infrastructure (PKI), which is resource consuming. In this paper, we propose an Identity-Based Batch Provable Data Possession (ID-BPDP) scheme to eliminate the certificate management. Meanwhile, to the best of our knowledge, it is the first identity-based provable data possession scheme supporting batch verification for multiple owners and multiple clouds simultaneously to reduce computation cost greatly. We prove that our scheme is correct and secure based on bilinear pairings and the hardness assumption of Computational Diffie-Hellman problem, and also give detailed analyses to show that our scheme is able to verify the integrity of data efficiently.
An Adaptive Wavelet Collocation Method for Solution of the Convection-Dominated Problem on a Sphere
Authors: Behera Ratikanta , Mehra Mani
Published: February 2018

In this paper, we present a numerical simulation of a convection-dominated problem using adaptive wavelet collocation method. This is based on second-generation spherical wavelets on a recursively refined spherical geodesic grid. The power lies in the fact that they only require small number of coefficients to represent this problem more accurately, which allows compression and efficient computation. In contrast with other approximate schemes, this approach reduced the number of collocation points used by factors of many orders of magnitude when compared to uniform grids of equivalent resolution. Numerical results demonstrate the efficiency of our approach.
Computed Tomography Scanning for Early Detection of Lung Cancer
Authors: Jacobson Francine L. , Jaklitsch Michael T.
Published: February 2018

Parallel and often unrelated developments in health care and technology have all been necessary to bring about early detection of lung cancer and the opportunity to decrease mortality from lung cancer through early detection of the disease by computed tomography. Lung cancer screening programs provide education for patients and clinicians, support smoking cessation as primary prevention for lung cancer, and facilitate health care for tobacco-associated diseases, including cardiovascular and chronic lung diseases. Guidelines for lung cancer screening will need to continue to evolve as additional risk factors and screening tests are developed. Data collection from lung cancer screening programs is vital to the further development of fiscally responsible guidelines to increase detection of lung cancer, which may include small groups with elevated risk for reasons other than tobacco exposure.
Common Cardiovascular Complications of Cancer Therapy: Epidemiology, Risk Prediction, and Prevention
Authors: Narayan Vivek , Ky Bonnie
Published: February 2018

There is growing awareness of the overlap between oncologic and cardiovascular (CV) diseases, including a wide range of CV effects of anticancer therapies. As novel anticancer therapeutics become available and cancer survival outcomes improve, the CV implications of cancer therapy become increasingly important. In addition to outlining the CV effects of commonly used cancer therapies and their consequences for long-term survivorship, this review highlights the recent efforts to improve the risk prediction and prevention of CV toxicity through the evaluation of sensitive measures for early toxicity detection and the implementation of cardioprotective strategies.
Measuring Patient-Reported Outcomes: Key Metrics in Reconstructive Surgery
Authors: Voineskos Sophocles H. , Nelson Jonas A. , Klassen Anne F. , Pusic Andrea L.
Published: February 2018

Satisfaction and improved quality of life are among the most important outcomes for patients undergoing plastic and reconstructive surgery for a variety of diseases and conditions. Patient-reported outcome measures (PROMs) are essential tools for evaluating the benefits of newly developed surgical techniques. Modern PROMs are being developed with new psychometric approaches, such as Rasch Measurement Theory, and their measurement properties (validity, reliability, responsiveness) are rigorously tested. These advances have resulted in the availability of PROMs that provide clinically meaningful data and effectively measure functional as well as psychosocial outcomes. This article guides the reader through the steps of creating a PROM and highlights the potential research and clinical uses of such instruments. Limitations of PROMs and anticipated future directions in this field are discussed.
CC chemokine receptor 2 promotes recruitment of myeloid cells associated with insulin resistance in nonalcoholic fatty liver disease
Authors: Parker Richard , Weston Christopher J. , Miao Zhenhua , Corbett Christopher , Armstrong Matthew J. , Ertl Linda , Ebsworth Karen , Walters Matthew J. , Baumart Trageen , Newland Dale , McMahon Jeff , Zhang Penglie , Singh Rajinder , Campbell James , Newsome Philip N. , Charo Israel , Schall Thomas J. , Adams David H.
Published: February 2018

Nonalcoholic fatty liver disease (NAFLD) is a common disease, closely associated with obesity and insulin resistance. We investigated the presence of a subset of myeloid cells associated with metabolic disturbance in the liver of patients with NAFLD and a murine model of obesity-induced liver disease. Gene and protein expression in liver and serum was investigated with RT-PCR or ELISA and correlated to clinical disease. Liver-infiltrating immune cells were isolated from normal or diseased human liver for flow cytometric analysis. In animal experiments, mice were fed a high-fat diet (60% of calories from fat) for 16 wk, or high-fat diet with 30% fructose for 32 wk to induce steatohepatitis and fibrosis. A small molecule inhibitor of CC chemokine receptor 2 (CCR2), CCX872, was administered to some mice. A subset of CD11c+CD206+ immune cells was enriched in human liver tissue, and greater infiltration was observed in NAFLD. The presence of CD11c+CD206+ myeloid cells correlated with systemic insulin resistance. CD11c+CD206+ cells expressed high levels of CCR2, and liver CC chemokine ligand 2 (CCL2) expression was increased in nonalcoholic steatohepatitis and correlated with disease activity. In mice, CCR2 inhibition reduced infiltration of liver CD11b+CD11c+F4/80+ monocytes, which are functional homologs of human CD11c+CD206+ cells, and improved liver injury and glycemic control. A role for CCR2/CCL2 in human NAFLD has long been postulated. These data confirm a role for this chemokine/receptor axis, through mediating adipose and hepatic infiltration of myeloid cells. Inhibition of CCR2 improved hepatic inflammation and fibrosis in murine models of NAFLD. These data confirm the rationale for targeting CCR2 to treat NAFLD.

NEW & NOTEWORTHY These data show for the first time that CD11c+CD206+ myeloid cells, previously associated with human adipose tissue inflammation, infiltrate into liver tissue in nonalcoholic fatty liver disease. These cells express CCR2. Inhibition of CCR2 in mice inhibits hepatic inflammation caused by a murine homolog of these myeloid cells and improves experimental liver disease.
Drug-induced pulmonary arterial hypertension: a primer for clinicians and scientists
Authors: Orcholski Mark E. , Yuan Ke , Rajasingh Charlotte , Tsai Halley , Shamskhou Elya A. , Dhillon Navneet K. , Voelkel Norbert F. , Zamanian Roham T. , de Jesus Perez Vinicio A.
Published: February 2018

Drug-induced pulmonary arterial hypertension (D-PAH) is a form of World Health Organization Group 1 pulmonary hypertension (PH) defined by severe small vessel loss and obstructive vasculopathy, which leads to progressive right heart failure and death. To date, 16 different compounds have been associated with D-PAH, including anorexigens, recreational stimulants, and more recently, several Food and Drug Administration-approved medications. Although the clinical manifestation, pathology, and hemodynamic profile of D-PAH are indistinguishable from other forms of pulmonary arterial hypertension, its clinical course can be unpredictable and to some degree dependent on removal of the offending agent. Because only a subset of individuals develop D-PAH, it is probable that genetic susceptibilities play a role in the pathogenesis, but the characterization of the genetic factors responsible for these susceptibilities remains rudimentary. Besides aggressive treatment with PH-specific therapies, the major challenge in the management of D-PAH remains the early identification of compounds capable of injuring the pulmonary circulation in susceptible individuals. The implementation of pharmacovigilance, precision medicine strategies, and global warning systems will help facilitate the identification of high-risk drugs and incentivize regulatory strategies to prevent further outbreaks of D-PAH. The goal for this review is to inform clinicians and scientists of the prevalence of D-PAH and to highlight the growing number of common drugs that have been associated with the disease.
Individualized estimation of human core body temperature using noninvasive measurements
Authors: Laxminarayan Srinivas , Rakesh Vineet , Oyama Tatsuya , Kazman Josh B. , Yanovich Ran , Ketko Itay , Epstein Yoram , Morrison Shawnda , Reifman Jaques
Published: February 2018

A rising core body temperature (Tc) during strenuous physical activity is a leading indicator of heat-injury risk. Hence, a system that can estimate Tcin real time and provide early warning of an impending temperature rise may enable proactive interventions to reduce the risk of heat injuries. However, real-time field assessment of Tcrequires impractical invasive technologies. To address this problem, we developed a mathematical model that describes the relationships between Tcand noninvasive measurements of an individual’s physical activity, heart rate, and skin temperature, and two environmental variables (ambient temperature and relative humidity). A Kalman filter adapts the model parameters to each individual and provides real-time personalized Tcestimates. Using data from three distinct studies, comprising 166 subjects who performed treadmill and cycle ergometer tasks under different experimental conditions, we assessed model performance via the root mean squared error (RMSE). The individualized model yielded an overall average RMSE of 0.33 (SD = 0.18)°C, allowing us to reach the same conclusions in each study as those obtained using the Tcmeasurements. Furthermore, for 22 unique subjects whose Tcexceeded 38.5°C, a potential lower Tclimit of clinical relevance, the average RMSE decreased to 0.25 (SD = 0.20)°C. Importantly, these results remained robust in the presence of simulated real-world operational conditions, yielding no more than 16% worse RMSEs when measurements were missing (40%) or laden with added noise. Hence, the individualized model provides a practical means to develop an early warning system for reducing heat-injury risk.

NEW & NOTEWORTHY A model that uses an individual’s noninvasive measurements and environmental variables can continually “learn” the individual’s heat-stress response by automatically adapting the model parameters on the fly to provide real-time individualized core body temperature estimates. This individualized model can replace impractical invasive sensors, serving as a practical and effective surrogate for core temperature monitoring.
Body map of regional vs. whole body sweating rate and sweat electrolyte concentrations in men and women during moderate exercise-heat stress
Authors: Baker Lindsay B. , Ungaro Corey T. , Sopeña Bridget C. , Nuccio Ryan P. , Reimel Adam J. , Carter James M. , Stofan John R. , Barnes Kelly A.
Published: February 2018

This study determined the relations between regional (REG) and whole body (WB) sweating rate (RSR and WBSR, respectively) as well as REG and WB sweat Na+concentration ([Na+]) during exercise. Twenty-six recreational athletes (17 men, 9 women) cycled for 90 min while WB sweat [Na+] was measured using the washdown technique. RSR and REG sweat [Na+] were measured from nine regions using absorbent patches. RSR and REG sweat [Na+] from all regions were significantly ( P < 0.05) correlated with WBSR ( r = 0.58–0.83) and WB sweat [Na+] ( r = 0.74–0.88), respectively. However, the slope and y-intercept of the regression lines for most models were significantly different than 1 and 0, respectively. The coefficients of determination ( r2) were 0.44–0.69 for RSR predicting WBSR [best predictors: dorsal forearm ( r2 = 0.62) and triceps ( r2 = 0.69)] and 0.55–0.77 for REG predicting WB sweat [Na+] [best predictors: ventral forearm ( r2 = 0.73) and thigh ( r2 = 0.77)]. There was a significant ( P < 0.05) effect of day-to-day variability on the regression model predicting WBSR from RSR at most regions but no effect on predictions of WB sweat [Na+] from REG. Results suggest that REG cannot be used as a direct surrogate for WB sweating responses. Nonetheless, the use of regression equations to predict WB sweat [Na+] from REG can provide an estimation of WB sweat [Na+] with an acceptable level of accuracy, especially using the forearm or thigh. However, the best practice for measuring WBSR remains conventional WB mass balance calculations since prediction of WBSR from RSR using absorbent patches does not meet the accuracy or reliability required to inform fluid intake recommendations.

NEW & NOTEWORTHY This study developed a body map of regional sweating rate and regional (REG) sweat electrolyte concentrations and determined the effect of within-subject (bilateral and day-to-day) and between-subject (sex) factors on the relations between REG and the whole body (WB). Regression equations can be used to predict WB sweat Na+concentration from REG, especially using the forearm or thigh. However, prediction of WB sweating rate from REG sweating rate using absorbent patches does not reach the accuracy or reliability required to inform fluid intake recommendations.
Unusual Cause of Acute Scrotal Pain-Inflammatory Noncommunicating Hydrocele: A Pediatric Case Report
Authors: Moritoki Yoshinobu , Mizuno Kentaro , Kato Taiki , Yasui Takahiro , Hayashi Yutaro
Published: February 2018

The etiology of scrotal pain is clinically classified in terms of the necessity for emergency surgery. Lately, color Doppler ultrasonography has reduced unnecessary surgeries, but there are still some cases that require immediate exploration because of an uncertain diagnosis. Here, we describe the case of a 14-month-old boy, who could not deliver his complaint accurately, presenting with a grumpy mood and a red swollen scrotum. Emergency surgery revealed that the cause was intense inflammation of the hydrocele wall, which typically does not cause acute scrotum. We also reviewed rare etiologies of scrotal pain for general physicians to develop the differential diagnosis.
Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum
Authors: Wang Donghui , Fan Bei , Wang Yan , Zhang Lijing , Wang Fengzhong
Published: February 2018

Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum. The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g. Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method. The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions. The molecular weight, polydispersity, and conformation of these fractions were also characterized. In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant. This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
Evolution of Longitudinal Resistance Performance of Granular Ballast Track with Durable Dynamic Reciprocated Changes
Authors: Xiao Jieling , Liu Hao , Wang Ping , Liu Ganzhong , Xu Jingmang , Chen Rong
Published: February 2018

A full‐scale test model with a distinct loading system is developed to analyze the dynamic change process of ballast beds under cyclic longitudinal reciprocated loading. The test analysis methodology accounts for the service performance, longitudinal resistances, and evolution trend of ballast beds under long‐term loading. The analysis shows that the ballast resistance‐displacement curves under cyclic loading are a set of closed hysteretic curves, indicating obvious energy consumption. In particular, a granular ballast bed is subject to cyclic softening during the cyclic process and the ballast longitudinal resistances degenerate obviously. More particularly, the cyclic softening of a granular ballast bed is dependent on the dynamic disturbance amplitude—the higher the dynamic disturbance amplitude, the more severe the cyclic softening will become. Moreover, this methodology contributes to forming a foundation for an in‐depth understanding of the dynamic service performance of ballast CWR tracks and of the stress deformation mechanism of continuously welded rail tracks.
Early Intervention and Nonpharmacological Therapy of Myopia in Young Adults
Authors: Zorena Katarzyna , Gładysiak Aleksandra , Ślęzak Daniel
Published: February 2018

Myopia is a condition of the eye where parallel rays focus in front of, instead of on, the retina, which results in excessive refractive power of the cornea or the lens or eyeball elongation. Studies carried out in recent years show that the etiology of myopia is complex with genetic and environmental factors playing a role. Refraction defects decrease the quality of vision, while progressing myopia can lead to partial loss of vision, which can be particularly dramatic in young adults. Therefore, it is so crucial to take appropriate actions aimed at preventing myopia progression. This is a review of nonpharmacological therapeutic possibilities of refraction defect prevention in young adults, with special regard to myofascial therapy, osteopathy, and massage of acupuncture points surrounding the eye.
The Impact of Pinless Navigation in Conventionally Aligned Total Knee Arthroplasty
Authors: Koenen P. , Schneider M. M. , Pfeiffer T. R. , Bouillon B. , Bäthis H.
Published: February 2018

Background. Restoration of the mechanical axis is a main objective in total knee replacement (TKR). Aim of this study was to analyse the verification tool of a pinless navigation system in conventional TKR (cTKR). Methods. In a prospective study, 147 TKR were performed by conventional technique. Using the “pinless verification” mode of a smartphone based navigation system, the cutting block position and final resection plane for distal femur and proximal tibial resection were measured. If necessary, the block position or resection level were optimized, corrections were protocolled. Postoperatively, standardized radiographs were performed. Results. In 65.3%, intraoperative measurements changed the surgical procedure (corrections: 20.4% femoral, 25.9% tibial, 19% both). The additional time for surgery compared to cTKR averaged 6 minutes (79 ± 15 versus 73 ± 17 minutes). Using navigation data, the final femoral and tibial axes were in 93% within a range of ±2°. A mean difference of 1.4° and 1.6° could be shown between the final measurement of the navigation system and the postoperative mLDFA and mMPTA. Conclusion. Intraoperative pinless navigation has impact on the surgical procedure in the majority of cTKR. It represents a less time-consuming tool to improve implant position while maintaining the routine of conventional technique.
The Role of Phospholipase C Signaling in Macrophage-Mediated Inflammatory Response
Authors: Zhu Liqian , Jones Clinton , Zhang Gaiping
Published: February 2018

Macrophages are crucial members of the mononuclear phagocyte system essential to protect the host from invading pathogens and are central to the inflammatory response with their ability to acquire specialized phenotypes of inflammatory (M1) and anti-inflammatory (M2) and to produce a pool of inflammatory mediators. Equipped with a broad range of receptors, such as Toll-like receptor 4 (TLR4), CD14, and Fc gamma receptors (FcγRs), macrophages can efficiently recognize and phagocytize invading pathogens and secrete cytokines by triggering various secondary signaling pathways. Phospholipase C (PLC) is a family of enzymes that hydrolyze phospholipids, the most significant of which is phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. Cleavage at the internal phosphate ester generates two second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), both of which mediate in diverse cellular functions including the inflammatory response. Recent studies have shown that some PLC isoforms are involved in multiple stages in TLR4-, CD14-, and FcγRs-mediated activation of nuclear factor kappa B (NF-κB), mitogen-activated protein kinase (MAPK), and interferon regulatory factors (IRFs), all of which are associated with the regulation of the inflammatory response. Therefore, secondary signaling by PLC is implicated in the pathogenesis of numerous inflammatory diseases. This review provides an overview of our current knowledge on how PLC signaling regulates the macrophage-mediated inflammatory response.
Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay
Authors: Wu Shilian , Zhang Yanqiong , Yang Long , Li Can-Peng
Published: February 2018

This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY (4.9×104 M−1) was greater than that of CB7/luteolin (3.2×103 M−1) or CB7/EGCG (4.8×103 M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety.
Effect of Ultrasonic Treatment Combined with Peracetic Acid Treatment Reduces Decay and Maintains Quality in Loquat Fruit
Authors: Ling Chen , Xu Jia , Shao Shuang , Wang Li , Jin Peng , Zheng Yonghua
Published: February 2018

The effects of ultrasonic treatment (UT, 400 watts for 6 min) combined with peracetic acid (PA, 0.4% (W/W)) treatment on fruit decay, browning, and quality and physiological changes in loquat fruit stored at 20°C were investigated. The results showed that treatment with UT or PA alone significantly reduced both decay and browning index in loquat fruit compared with the control. The combined treatment was more effective in decreasing decay and browning index and maintaining higher quality parameters than UT or PA treatment alone. Loquat fruits treated with the combined treatment maintained higher activities of polyphenol oxidase (PPO) and peroxidase (POD) than those treated with other treatments. In addition, UT combined with PA treatment significantly reduced the increase of firmness and increased fruit extractable juice, total soluble solid (TSS), and ascorbic acid content in loquat fruit. Moreover, the contents of total phenolics and total flavonoids were enhanced by the combination of UT and PA treatment. The combination of UT and PA treatment could be a useful method to reduce decay and browning in loquat fruit stored at room temperature, which has potential for application in the loquat industry.
Combined Phacoemulsification and Goniosynechialysis under an Endoscope for Chronic Primary Angle-Closure Glaucoma
Authors: Nie Li , Pan Weihua , Fang Aiwu , Li Zhangliang , Qian Zhenbin , Fu Lin , Chan Yau Kei
Published: February 2018

Purpose. To investigate the clinical efficacy and safety of combined phacoemulsification with goniosynechialysis (GSL) under an ophthalmic endoscope for chronic primary angle-closure glaucoma and coexisting cataract. Methods. This is a retrospective study. The intraocular pressure (IOP), best-corrected visual acuity (BCVA), and number of glaucoma medications at baseline and each postoperative follow-up visit were recorded. Other measurements included supraciliochoroidal fluid measured by anterior segment optical coherence tomography, corneal endothelial cell density (ECD), and peripheral anterior synechia (PAS). All patients were followed for more than a year. Results. Thirty-eight eyes of 31 patients were included. The mean follow-up duration was 16.3 ± 3.9 months. The IOP decreased from 22.2 ± 9.3 mmHg at baseline to 15.4 ± 4.2 mmHg at the last follow-up (P<0.001). The mean number of glaucoma medications (0.1 ± 0.6) at the last follow-up was significantly lower than the preoperative number (2.3 ± 1.1) (P<0.001). All patients achieved improved or stable visual acuity after surgery. All patients achieved a complete opened angle after GSL. The postoperative complications included hyphema (7.9%), exudation (5.3%), transiently elevated IOP (55.3%), and supraciliochoroidal fluid (40%). Conclusions. Combined phacoemulsification and GSL under an endoscope can completely reopen PAS and is an effective and safe method for patients with chronic primary angle-closure glaucoma and coexisting cataract.
Chicken Coccidiosis in Central Java, Indonesia: A Recent Update
Authors: Hamid Penny Humaidah , Kristianingrum Yuli Purwandari , Wardhana April Hari , Prastowo Sigit , Silva Liliana Machado Ribeiro da
Published: February 2018

Avian coccidiosis is a huge problem worldwide. Heavily infected animals that show severe clinical signs and coccidiostat resistance are causing important economic losses. The present study aimed to update the recent cases of coccidiosis in Central Java, Indonesia, and to show the importance of the disease in the region. A total of 699 samples were obtained from different chicken breed. Different Eimeria species were detected in 175 individuals (25.04%). Three different groups of chicken breed were considered: local chicken (autochthonous chickens of Sentul and Jawa), commercial broiler, and layer. Broiler chickens showed the highest prevalence of infection (34%), followed by layer (26.26%) and local chickens (10.45%). Mild to severe clinical signs of avian coccidiosis were observed in 42% of the infected animals, while 58% of the infected animals showed no clinical signs other than low feed conversion rates. Seven different Eimeria species were identified: E. tenella was the most prevalent (43.3%), followed by E. maxima (26.3%), E. necatrix (15.7%), E. acervulina (8%), E. praecox (3.1%), E. mitis (2.2%), and E. brunetti (1.3%). Coinfections with several Eimeria species were diagnosed. With this study we found massive usage of coccidiostat in the region even though its usage cannot guarantee coccidiosis-free chicken production.
Complex Dynamics of a Discrete-Time Predator-Prey System with Ivlev Functional Response
Authors: Baek Hunki
Published: February 2018

The dynamics of a discrete-time predator-prey system with Ivlev functional response is investigated in this paper. The conditions of existence for flip bifurcation and Hopf bifurcation in the interior of R+2 are derived by using the center manifold theorem and bifurcation theory. Numerical simulations are presented not only to substantiate our theoretical results but also to illustrate the complex dynamical behaviors of the system such as attracting invariant circles, periodic-doubling bifurcation leading to chaos, and periodic-halving phenomena. In addition, the maximum Lyapunov exponents are numerically calculated to confirm the dynamical complexity of the system. Finally, we compare the system to discrete systems with Holling-type functional response with respect to dynamical behaviors.
Application of Linear Viscoelastic Properties in Semianalytical Finite Element Method with Recursive Time Integration to Analyze Asphalt Pavement Structure
Authors: Liu Pengfei , Xing Qinyan , Wang Dawei , Oeser Markus
Published: February 2018

Traditionally, asphalt pavements are considered as linear elastic materials in finite element (FE) method to save computational time for engineering design. However, asphalt mixture exhibits linear viscoelasticity at small strain and low temperature. Therefore, the results derived from the elastic analysis will inevitably lead to discrepancies from reality. Currently, several FE programs have already adopted viscoelasticity, but the high hardware demands and long execution times render them suitable primarily for research purposes. Semianalytical finite element method (SAFEM) was proposed to solve the abovementioned problem. The SAFEM is a three‐dimensional FE algorithm that only requires a two‐dimensional mesh by incorporating the Fourier series in the third dimension, which can significantly reduce the computational time. This paper describes the development of SAFEM to capture the viscoelastic property of asphalt pavements by using a recursive formulation. The formulation is verified by comparison with the commercial FE software ABAQUS. An application example is presented for simulations of creep deformation of the asphalt pavement. The investigation shows that the SAFEM is an efficient tool for pavement engineers to fast and reliably predict asphalt pavement responses; furthermore, the SAFEM provides a flexible, robust platform for the future development in the numerical simulation of asphalt pavements.
Clinical Outcomes after Conservative Management of Cervical Intraepithelial Neoplasia Grade 2 (CIN2) in Women Ages 21–39 Years
Authors: Silver Michelle I. , Gage Julia C. , Schiffman Mark , Fetterman Barbara , Poitras Nancy E. , Lorey Thomas , Cheung Li C. , Katki Hormuzd A. , Locke Alexander , Kinney Walter K. , Castle Philip E.
Published: February 2018

Cervical intraepithelial neoplasia grade 2 (CIN2) frequently regresses, is typically slow-growing, and rarely progresses to cancer. Some women forgo immediate treatment, opting for conservative management (heightened surveillance with cytology and colposcopy), to minimize overtreatment and increased risk of obstetric complications; however, there are limited data examining clinical outcomes in these women. We performed a retrospective cohort analysis of younger women diagnosed with initially untreated CIN1/2, CIN2 and CIN2/3 lesions at Kaiser Permanente Northern California between 2003 and 2015. Clinical outcomes were categorized into five mutually exclusive hierarchical groups: cancer, treated, returned to routine screening, persistent high-grade lesion, or persistent low-grade lesion. Median follow-up for the 2,417 women was 48 months. Six women were diagnosed with cancer (0.2%), all with history of high-grade cytology, and none after a negative cotest. Thirty percent of women were treated, and only 20% returned to routine screening; 50% remained in continued intensive follow-up, of which 86% had either low-grade cytology/histology or high-risk human papillomavirus (HPV) positivity, but not necessarily persistence of a single HPV type. No cancers were detected after a single negative cotest in follow-up. Almost half of initially untreated women did not undergo treatment, but remained by protocol in colposcopy clinic for 2 or more years in the absence of persisting CIN2+. Their incomplete return to total negativity was possibly due to sequential new and unrelated low-grade abnormalities. The prolonged colposcopic surveillance currently required to return to routine screening in the absence of persisting CIN2+ might not be necessary after a negative cotest.

Significance: Many younger women under conservative management following an initial CIN2 result remain in a clinical protocol of prolonged intensified surveillance without a subsequent diagnosis of CIN2 or more severe diagnoses. More research is needed to determine whether such prolonged management might be unnecessary following a negative cotest for those women with an initial CIN2 but otherwise only low-grade findings. Cancer Prev Res; 11(3); 165–70. ©2018 AACR.