bluuebunny/crossref_metadata_2025 huggingface dataset:
Advancements in Research on Genetic Kidney Diseases Using Human-Induced Pluripotent Stem Cell-Derived Kidney Organoids
Authors: Na Do Hyun , Cui Sheng , Fang Xianying , Lee Hanbi , Eum Sang Hun , Shin Yoo Jin , Lim Sun Woo , Yang Chul Woo , Chung Byung Ha
Published: July 2024

Genetic or hereditary kidney disease stands as a pivotal cause of chronic kidney disease (CKD). The proliferation and widespread utilization of DNA testing in clinical settings have notably eased the diagnosis of genetic kidney diseases, which were once elusive but are now increasingly identified in cases previously deemed CKD of unknown etiology. However, despite these diagnostic strides, research into disease pathogenesis and novel drug development faces significant hurdles, chiefly due to the dearth of appropriate animal models and the challenges posed by limited patient cohorts in clinical studies. Conversely, the advent and utilization of human-induced pluripotent stem cells (hiPSCs) offer a promising avenue for genetic kidney disease research. Particularly, the development of hiPSC-derived kidney organoid systems presents a novel platform for investigating various forms of genetic kidney diseases. Moreover, the integration of the CRISPR/Cas9 technique into this system holds immense potential for efficient research on genetic kidney diseases. This review aims to explore the applications of in vitro kidney organoids generated from hiPSCs in the study of diverse genetic kidney diseases. Additionally, it will delve into the limitations of this research platform and outline future perspectives for advancing research in this crucial area.
Perineural Invasion in Cervical Cancer: A Hidden Trail for Metastasis
Authors: Chen Guoqiang , Sun Hao , Chen Yunxia , Wang Li , Song Ouyi , Zhang Jili , Li Dazhi , Liu Xiaojun , Feng Lixia
Published: July 2024

Perineural invasion (PNI), the neoplastic invasion of nerves, is an often overlooked pathological phenomenon in cervical cancer that is associated with poor clinical outcomes. The occurrence of PNI in cervical cancer patients has limited the promotion of Type C1 surgery. Preoperative prediction of the PNI can help identify suitable patients for Type C1 surgery. However, there is a lack of appropriate preoperative diagnostic methods for PNI, and its pathogenesis remains largely unknown. Here, we dissect the neural innervation of the cervix, analyze the molecular mechanisms underlying the occurrence of PNI, and explore suitable preoperative diagnostic methods for PNI to advance the identification and treatment of this ominous cancer phenotype.
Transformation towards a Low-Emission and Energy-Efficient Economy Realized in Agriculture through the Increase in Controllability of the Movement of Units Mowing Crops While Simultaneously Discing Their Stubble
Authors: Orynycz Olga , Nadykto Volodymyr , Kyurchev Volodymyr , Tucki Karol , Kulesza Ewa
Published: July 2024

When harvesting cereals and fodder grasses, a two-phase method is often used. This process is carried out using trailed and suspended collecting units. The former are asymmetrical and often pose problems regarding the stability of their movement in the horizontal plane. In practice, suspended harvesting units with a front-mounted header are becoming more and more widely used. The disadvantage of their use is that the soil is exposed after passing through the space between the swaths of the mown crop. This is followed by an intense loss of moisture. In order to eliminate this shortcoming, a collecting unit was proposed, consisting of a tractor with a front attachment and a disc harrow mounted at the rear. An appropriate mathematical model was developed to justify the scheme and parameters of such a unit. In this case, this model is used to assess the controllability of the movement of the dynamic system under the influence of control action in the form of the angular rotation of the tractor’s steered wheels. As a result of mathematical modelling, it was found that satisfactory controllability of the movement of the harvesting units can be ensured by acting on the tractor’s driven wheels with a frequency of 0–1 s−1 and a working speed of close to 3 m·s−1. In this case, it is desirable to set the deflection resistance coefficient of the rear tyres of the tractor (and therefore, the air pressure in them) to a smaller value, and that of the front tyres to a larger value. This helps both to improve the movement controllability of the harvesting unit and to reduce its energy consumption by an average of 6.75%. The emissivity of selected harmful chemicals and particulates emitted by the harvesting unit, depending on the fuel burned, was also examined. The way in which the use of the harvesting unit affects the reduction of emissions of harmful compounds into the atmosphere was also revealed.
Multi-Scenario Research on the Coupled and Coordinated Development of the Economic–Energy–Environmental (3E) System under the Reconstruction of the Power System—New Exploration Based on the “Dual Triangle” Theory
Authors: Cen Haifeng , Wang Wenxiu , Chen Liping , Hao Weitao , Guan Zhe , Lu Juntong , Cai Guotian
Published: July 2024

Under the background of building a new-type power system, as the core of the energy system, the restructuring of the power system will inevitably have a linked impact on the coordinated development of the economy–energy–environment system. A “Dual Triangle” theoretical framework consisting of the “Energy Impossibility Triangle” and “Sustainable Development Triangle” was constructed in this paper. By combining historical statistical analysis with scenario predictive analysis, the historical characteristics of the “Dual Triangle” and future trends under different scenarios were analyzed, and the coupled interaction relationship of the “Dual Triangle” was also studied. Conclusions were as follows: The energy system gradually inclined towards economy and cleanliness from 2010 to 2022, and the operation status of the “Dual Triangle” was stable. A positive coupling relationship within the “Dual Triangle” in vigorously promoting renewable energy scenarios is close, which can be regarded as the preferred path for the reconstruction of the power system. The security and low-carbon phased development scenario is a development model that dynamically mitigates the challenges of the “Energy Impossibility Triangle”. Security has become the core contradiction of the “Energy Impossibility Triangle”. This paper conducts an integrated and innovative application of the “Dual Triangle” theory and provides a case basis for how to achieve the coupled interaction of the “Dual Triangle”.
A Prototype Decision Support System for Tree Selection and Plantation with a Focus on Agroforestry and Ecosystem Services
Authors: Yadav Neelesh , Rakholia Shrey , Yosef Reuven
Published: July 2024

This study presents the development and application of a prototype decision support system (DSS) for tree selection specifically for Punjab, India, a region facing challenges of low forest cover and an increasing demand for sustainable land use practices. The DSS developed using the R Shiny framework integrates ecological, social, and agro-commercial criteria to facilitate scientific knowledge decision making in tree plantation. The modules in this DSS include a tree selection tool based on comprehensive species attributes, a GIS-based tree suitability map module utilizing an Analytical Hierarchical Process (AHP), and a silviculture practice information module sourced from authoritative databases. Combining sophisticated statistical and spatial analysis, such as NMDS and AHP-GIS, this DSS mitigates data redundancy in SDM while incorporating extensive bibliographic research in dataset processing. The study highlights the necessity of fundamental niche-based suitability in comparison to realized niche suitability. It emphasizes on the importance of addressing ecosystem services, agro-commercial aspects, and enhancing silvicultural knowledge. Additionally, the study underscores the significance of local stakeholder engagement in tree selection, particularly involving farmers and other growers, to ensure community involvement and support. The DSS supports agroforestry initiatives and finds applications in urban tree management and governmental programs, emphasizing the use of scientific literature at each step, in contrast to relying solely on local knowledge.
Impact of Management Measures on Multiple Ecosystem Function Trade-Offs and Their Dynamics in Subtropic Pinus massoniana Plantations
Authors: Zhang Kexin , Zou Hongzhu , Lei Xiangdong , Zeng Ji , Lan Jie , Guo Xiaoxue , Gao Dongli , Liu Xianzhao , Zhang Huiru
Published: July 2024

With the changing demands imposed on forests by human beings, optimizing forest management to fully utilize their multifunctionality has become a priority. Reasonable forest management measures can maintain stable forest ecosystems that fully coordinate the balance between ecological, societal, and economic aspects. As planted forests are the main application scenario of forest management worldwide, it is of great importance to understand the trade-offs between ecosystem functions and their dynamic changes in planted forests. This paper investigates the effects of different management measures on the ecosystem function of Pinus massoniana plantation forests in the subtropics. It examines four different management measures and explores how they impact multiple ecosystem function indexes and the trade-offs between ecosystem functions during forest restoration. The different management measures effectively promoted the studied ecosystem functions, with higher annual growth rates of the integrated functional indices for timber production, carbon sequestration, and biodiversity compared to the control. Over time, the ecosystem function interactions under the different management measures alternated between trade-offs and synergistic. Only the stand with a 65% harvesting intensity and replanting of various native broadleaf species was able to sustain the synergistic relationships among ecosystem functions, and the dominant function trended toward biodiversity. These observations of dynamic changes and interactions in ecosystem functions of Pinus massoniana plantation forests under various management measures will serve as a valuable reference for the sustainable management of these forests in subtropical regions.
Investigating the Potential of Polypore Fungi as Eco-Friendly Materials in Food Industry Applications
Authors: Pawłowicz Tomasz , Gabrysiak Karolina Anna , Wilamowski Konrad
Published: July 2024

Polyporoid fungi represent an untapped resource in the ancillary use of forests, traditionally utilized in both historic and contemporary medicine for their diverse bioactive properties, yet their potential for creating materials within the food industry remains largely unexplored. This article delves into the polyporoid fungi as a promising, yet underutilized, biomaterial resource for eco-friendly applications in the food sector. Despite their widespread use in traditional and modern medicine, the exploration of these fungi for industrial applications, particularly in food storage solutions and utensils, is in its nascent stages. The Białowieża Primeval Forest, characterized by its abundant deadwood and minimal human intervention, offers a rich repository of polyporoid fungi. This study aims to illuminate the ecological significance and potential industrial applications of polyporoid fungi. By reviewing existing research and synthesizing insights into the genetic diversity, biochemical capabilities, and ecological roles of polyporoid species such as Fomes fomentarius, Fomitopsis pinicola, and Trametes versicolor, this article proposes a novel approach to leveraging polyporoid fungi in developing sustainable solutions that meet current environmental and health-conscious trends. The investigation not only underscores the potential of polyporoid fungi in advancing green technologies but also highlights the importance of utilizing renewable resources in material science, fostering a shift towards more sustainable industrial practices.
Full-Length Transcriptome Assembly of Platycladus orientalis Root Integrated with RNA-Seq to Identify Genes in Response to Root Pruning
Authors: Dou Hao , Sun Huijuan , Feng Xi , Wang Tiantian , Wang Yilin , Quan Jin’e , Yang Xitian
Published: July 2024

Platycladus orientalis (P. orientalis) is a common tree used for vegetation restoration in northern China, and its large area propagation helps to improve site conditions. However, under harsh conditions such as poor land, the survival rate of P. orientalis is very low. Numerous studies have shown that root pruning can promote the formation of lateral roots in seedlings, enhancing the roots’ capacity to absorb soil nutrients and water, and thereby improving the survival rate of seedlings. In this study, a one-third root pruning treatment was applied to P. orientalis seedlings, and the whole transcriptome of seedlings subjected to both control (CK) and root pruning treatments was sequenced to analyze their gene expression profiles. This study investigated the regulatory mechanisms of lateral root development in response to root pruning damage at the molecular level. Using nine cells, 15.28 Gb of clean data were obtained, which yielded 101,688 high-quality full-length transcript sequences and 22,955 low-quality full-length transcript sequences after clustering. Redundancy was then removed using CD-HIT, and Illumina RNA-seq sequencing produced 139.26 Gb of clean data. A total of 2025 differentially expressed genes (DEGs) were identified at three time points following root pruning treatment. Enrichment analysis revealed that the peroxidase gene family plays a significant role in lateral root proliferation. Furthermore, the expression levels of the peroxidase gene family were notably upregulated in comparison to the control group. Pathway enrichment analysis identified 22 relevant genes, which appeared to be highly associated with root growth and resilience to stress. Through examining the expression patterns and correlations of these genes, five central genes emerged as key players. The findings of this research suggest that the peroxidase gene family plays a crucial role in the stress response and root development of P. orientalis, providing reference and guidance for root development in other plant species.
A Systematic Approach to Map and Evaluate the Wildfire Behavior at a Territorial Scale in the Northwestern Iberian Peninsula
Authors: Rincón Thais , Alonso Laura , Picos Juan , Molina-Terrén Domingo , Armesto Julia
Published: July 2024

In the current context of extreme wildfires, understanding fire behavior at a territorial level has proven crucial for territory planning. This type of analysis is usually conducted by analyzing past wildfire statistics. In this study, we forego the past information related to wildfires and analyze, instead, the behavior of the entire territory in the face of wildfires. This allows for the distribution of ignition points to be systematized and for typical and atypical weather scenarios to be considered. This analysis relies on the use of wildfire simulation software. Ignition points used for the simulations were distributed using a systematic 1 × 1 km grid throughout the whole study area. Wildfires were simulated for each ignition point using eight different weather scenarios representing both typical and atypical weather conditions. The fire behavior on the territory was analyzed using rate of spread and intensity parameters for each simulated wildfire. It was observed that this territory is extremely prone to large wildfires both in typical and atypical weather conditions and that there is a tendency for extreme behaviors to develop. Some features were identified as prevention issues that ought to be addressed. This study develops a strategy to evaluate, in a systematic manner, the response of the territory to the threat of wildfires.
Phenotypic Expansion of Autosomal Dominant LZTR1-Related Disorders with Special Emphasis on Adult-Onset Features
Authors: Uliana Vera , Ambrosini Enrico , Taiani Antonietta , Cesarini Sofia , Cannizzaro Ilenia Rita , Negrotti Anna , Serra Walter , Quintavalle Gabriele , Micale Lucia , Fusco Carmela , Castori Marco , Martorana Davide , Bortesi Beatrice , Belli Laura , Percesepe Antonio , Pisani Francesco , Barili Valeria
Published: July 2024

Leucine zipper-like transcription regulator 1 (LZTR1) acts as a negative factor that suppresses RAS function and MAPK signaling; mutations in this protein may dysregulate RAS ubiquitination and lead to impaired degradation of RAS superfamily proteins. Germline LZTR1 variants are reported in Noonan syndrome, either autosomal dominant or autosomal recessive, and in susceptibility to schwannomatosis. This article explores the genetic and phenotypic diversity of the autosomal dominant LZTR1-related disorders, compiling a cohort of previously published patients (51 with the Noonan phenotype and 123 with schwannomatosis) and presenting two additional adult-onset cases: a male with schwannomatosis and Parkinson’s disease and a female with Noonan syndrome, generalized joint hypermobility, and breast cancer. This review confirms that autosomal dominant LZTR1-related disorders exhibit an extreme phenotypic variability, ranging from relatively mild manifestations to severe and multi-systemic involvement, and offers updated frequences of each clinical feature. The aim is to precisely define the clinical spectrum of LZTR1-related diseases, using also two new emblematic clinical cases. Gaining insight into the mechanisms underneath this variability is crucial to achieve precision diagnostics and the development of therapeutic interventions.
Chloroplast Genome Profiling and Phylogenetic Insights of the “Qixiadaxiangshui” Pear (Pyrus bretschneideri Rehd.1)
Authors: Jiao Huijun , Chen Qiming , Xiong Chi , Wang Hongwei , Ran Kun , Dong Ran , Dong Xiaochang , Guan Qiuzhu , Wei Shuwei
Published: July 2024

The “Qixiadaxiangshui” pear (Pyrus bretschneideri Rehd.1) is a highly valued cultivar known for its crisp texture, abundant juice, and rich aroma. In this study, we reported the first complete chloroplast genome sequence of the “Qixiadaxiangshui” pear, which is 159,885 bp in length with a GC content of 36.58%. The genome exhibits a typical circular quadripartite structure, comprising a large single-copy region (LSC), a small single-copy region (SSC), and a pair of inverted repeat regions (IRs). A total of 131 genes were identified, including 84 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. We also identified 209 simple sequence repeats (SSRs) and several mutation hotspots, such as ndhC-trnM-CAU and trnR-UCU-atpA, which can be applied in molecular identification and phylogenetic studies of Pyrus. Comparative genomic analysis showed high conservation among ten pear cultivars. Phylogenetic analysis indicated that the “Qixiadaxiangshui” pear is closely related to germplasm Dangshansuli, Wonwhang, and Yali, suggesting a recent common ancestor. These findings provided valuable insights into the genetic diversity and evolutionary dynamics of the Pyrus species and contribute to the conservation and breeding of pear germplasm resources.
Exploring the Potential of Crotalaria juncea L. for Phytoremediation: Insights from Gas Exchange, Pigment Quantification, and Growth Measurements under Copper Stress
Authors: dos Santos Beatriz Silvério , Mendonça Gabriel Wanderley , Ferreira Tassia Caroline , Bomfim Nayane Cristina Pires , de Carvalho Isabella Fiorini , Aguilar Jailson Vieira , Camargos Liliane Santos
Published: July 2024

Soil contamination by trace elements is a worldwide concern that can result from several sources, such as mining, smelting, car traffic exhaust, agriculture plant protection products such as fungicides, and fertilizers. Among the metals involved, copper can cause alterations in the photosynthetic, respiratory, and enzymatic processes of plants, leading to reduced growth of roots and shoots. An alternative to dealing with metals present in the soil is phytoremediation, which consists of using plants to extract or stabilize these elements. The leguminous Crotalaria juncea is widely used as a green manure and may be advantageous due to its capacity for biological nitrogen fixation and biomass accumulation. This research aimed to evaluate the growth and physiological behaviour of C. juncea in copper-contaminated soil and its potential use as a phytoremediation plant. For the fresh and dry mass of shoots and roots, compared with 30 mg.dm−3 of Cu, there was a decrease in values with the increase in concentrations up to a dose of 480 mg.dm−3. The roots were less sensitive to increased Cu concentrations than the shoots. The tolerance index decreased as copper concentrations in the soil increased. From 60 mg.dm−3, its vegetative growth decreased, but C. Juncea was able to tolerate and accumulate copper in the root system, presenting high potential as a phytostabilizing species.
Exploring PCSWMM for Large Mixed Land Use Watershed by Establishing Monitoring Sites to Evaluate Stream Water Quality
Authors: Ansari Mohd Sohib , Sharma Suresh , Armstrong Felicia P. , Delisio Mark , Ehsani Sahar
Published: July 2024

Extensive hydrologic and water quality modeling within a watershed benefits from long-term flow and nutrient data sets for appropriate model calibration and validation. However, due to a lack of local water quality data, simpler water quality modeling techniques are generally adopted. In this study, the monitoring sites were established at two different locations to collect hydraulic data for the hydraulic calibration and validation of the model. In addition, water quality samples were collected at eight monitoring sites and analyzed in the lab for various parameters for calibration. This includes total suspended solids (TSS), soluble phosphorus, five-day biochemical oxygen demand (BOD5), and dissolved oxygen (DO). The Personal Computer Storm Water Management Model (PCSWMM) 7.6 software was used to simulate all the pollutant loads using event mean concentrations (EMCs). The performance of the model for streamflow calibration at the two USGS gauging stations was satisfactory, with Nash–Sutcliffe Efficiency (NSE) values ranging from 0.51 to 0.54 and coefficients of determination (R2) ranging from 0.71 to 0.72. The model was also validated with the help of historical flow data with NSE values ranging from 0.5 to 0.79, and R2 values ranging from 0.6 to 0.95. The hydraulic calibration also showed acceptable results with reasonable NSE and R2 values. The water quality data recorded at the monitoring stations were then compared with the simulated water quality modeling results. The model reasonably simulated the water quality, which was evaluated through visual inspection using a scatter plot. Our analysis showed that the upstream tributaries, particularly from agricultural areas, were contributing more pollutants than the downstream tributaries. Overall, this study demonstrates that the PCSWMM, which was typically used for modeling urban watersheds, could also be used for modeling larger mixed land use watersheds with reasonable accuracy.
Association between Depressive Symptoms, Physical Activity, and Health Factors in Hispanic Emerging Adults
Authors: Gutierrez Margaret , Palacios Cristina , Narayanan Vijaya , George Florence , Sales Martinez Sabrina
Published: July 2024

Physical activity is a modifiable lifestyle behavior known for reducing symptoms of and being a risk factor for depression and mental health disorders. However, emerging adults (ages 18–25) struggle to meet recommended amounts. In this study, we explore the association between physical activity, depressive symptoms, and health factors in 137 Hispanic emerging adults. Using a cross-sectional survey design, sociodemographic information, depressive symptoms (CES-D score), physical activity (IPAQ score), body composition, and blood pressure measures were obtained. Statistical analyses included correlation and regression analyses. More than half of the participants demonstrated depressive symptomology (59.1%) and body fat percentage greater than 25% (64.2%). Body fat percentage, lean body mass, stress, and heart rate demonstrated notable associations with depressive symptoms and physical activity. When measured continuously and categorically, IPAQ was not a significant predictor of depressive symptoms. When used as a binary variable with a cutoff of 600 MET min/week, IPAQ score revealed a negative relationship with CES-D score (β = −0.169, SE = 2.748, p = 0.034). Our results indicate that a threshold of physical activity, 600 MET min/week, may confer protective effects against depressive symptoms. Future research should investigate the context and quality of physical activity to address mental health disparities in this underrepresented population.
Strategies for Coping with Occupational Trauma: A Scoping Review of the Police Officer Context
Authors: Modula Mantji Juliah , Mathapo-Thobakgale Ellen Mokgobola , Nyoni Champion N. , Jansen Ronelle
Published: July 2024

Background: Occupational trauma is heightened among police officers due to their exposure to physical, biological, chemical, and psychological hazards. Sustained occupational trauma results in mental illness among members of the police, which is a public health issue of concern. This study aimed to report a scoping review of the literature on strategies employed by police officers for coping with occupational trauma around the globe. Methods: A search string, formulated from the review question of what is known about the strategies of police officers for coping with occupational trauma, was used to search for articles from databases. A total of 588 hits were screened against inclusion criteria, resulting in 36 full-text studies between 1983 and 2022 being included in this review. Data were extracted using a standardised data extraction tool. The multi-step process was used to analyse the extracted data, integrating quantitative and qualitative approaches. Results: From this review, ‘adaptive coping mechanisms’, involving confrontation; ‘maladaptive coping mechanisms’, such as self-isolation, distancing and substance use; ‘resilience’, relating to mental preparation, and ‘seeking support systems’ from family, colleagues and professionals reflected the strategies used by police officers to cope with occupational trauma. Social stigma related to mental health disorders impacts the strategies used by police officers to cope with occupational trauma. Conclusions: the police management and healthcare practitioners must collaborate towards providing constructive environments that support and strengthen police officers’ strategies for coping with occupational trauma.
Genome-Wide Characterization of Fructose 1,6-Bisphosphate Aldolase Genes and Expression Profile Reveals Their Regulatory Role in Abiotic Stress in Cucumber
Authors: Zhang Jinlong , Liu Yike , Zhou Zhenpeng , Yang Lina , Xue Zhanjun , Li Qingyun , Cai Bingbing
Published: July 2024

The fructose-1,6-bisphosphate aldolase (FBA) gene family exists in higher plants, with the genes of this family playing significant roles in plant growth and development, as well as response to abiotic stresses. However, systematic reports on the FBA gene family and its functions in cucumber are lacking. In this study, we identified five cucumber FBA genes, named CsFBA1-5, that are distributed randomly across chromosomes. Phylogenetic analyses involving these cucumber FBAs, alongside eight Arabidopsis FBA proteins and eight tomato FBA proteins, were conducted to assess their homology. The CsFBAs were grouped into two clades. We also analyzed the physicochemical properties, motif composition, and gene structure of the cucumber FBAs. This analysis highlighted differences in the physicochemical properties and revealed highly conserved domains within the CsFBA family. Additionally, to explore the evolutionary relationships of the CsFBA family further, we constructed comparative syntenic maps with Arabidopsis and tomato, which showed high homology but only one segmental duplication event within the cucumber genome. Expression profiles indicated that the CsFBA gene family is responsive to various abiotic stresses, including low temperature, heat, and salt. Taken together, the results of this study provide a theoretical foundation for understanding the evolution of and future research into the functional characterization of cucumber FBA genes during plant growth and development.
Transcriptomics Provide Insights into Early Responses to Sucrose Signaling in Lupinus albus, a Model Plant for Adaptations to Phosphorus and Iron Deficiency
Authors: Shammi Tahmina , Lee Yishen , Trivedi Jayati , Sierras Dakota , Mansoor Aniqua , Maxwell Jason M. , Williamson Matthew , McMillan Mark , Chakravarty Indrani , Uhde-Stone Claudia
Published: July 2024

Phosphorus (P) and iron (Fe) deficiency are major limiting factors for plant productivity worldwide. White lupin (Lupinus albus L.) has become a model plant for understanding plant adaptations to P and Fe deficiency, because of its ability to form cluster roots, bottle-brush-like root structures play an important role in the uptake of P and Fe from soil. However, little is known about the signaling pathways involved in sensing and responding to P and Fe deficiency. Sucrose, sent in increased concentrations from the shoot to the root, has been identified as a long-distance signal of both P and Fe deficiency. To unravel the responses to sucrose as a signal, we performed Oxford Nanopore cDNA sequencing of white lupin roots treated with sucrose for 10, 15, or 20 min compared to untreated controls. We identified a set of 17 genes, including 2 bHLH transcription factors, that were up-regulated at all three time points of sucrose treatment. GO (gene ontology) analysis revealed enrichment of auxin and gibberellin responses as early as 10 min after sucrose addition, as well as the emerging of ethylene responses at 20 min of sucrose treatment, indicating a sequential involvement of these hormones in plant responses to sucrose.
Pharmacotherapeutic Considerations on Telomere Biology: The Positive Effect of Pharmacologically Active Substances on Telomere Length
Authors: Apetroaei Miruna-Maria , Fragkiadaki Persefoni , Velescu Bruno Ștefan , Baliou Stella , Renieri Elisavet , Dinu-Pirvu Cristina Elena , Drăgănescu Doina , Vlăsceanu Ana Maria , Nedea Marina Ionela (Ilie) , Udeanu Denisa Ioana , Docea Anca Oana , Tsatsakis Artistidis , Arsene Andreea Letiția
Published: July 2024

Telomeres are part of chromatin structures containing repeated DNA sequences, which function as protective caps at the ends of chromosomes and prevent DNA degradation and recombination, thus ensuring the integrity of the genome. While telomere length (TL) can be genetically inherited, TL shortening has been associated with ageing and multiple xenobiotics and bioactive substances. TL has been characterised as a reliable biomarker for the predisposition to developing chronic pathologies and their progression. This narrative review aims to provide arguments in favour of including TL measurements in a complex prognostic and diagnostic panel of chronic pathologies and the importance of assessing the effect of different pharmacologically active molecules on the biology of telomeres. Medicines used in the management of cardiovascular diseases, diabetes, schizophrenia, hormone replacement therapy at menopause, danazol, melatonin, and probiotics have been studied for their positive protective effects against TL shortening. All these classes of drugs are analysed in the present review, with a particular focus on the molecular mechanisms involved.
Thermopriming Induces Time-Limited Tolerance to Salt Stress
Authors: Körner Tobias , Zinkernagel Jana , Röhlen-Schmittgen Simone
Published: July 2024

Implementing sustainable crop protection practices is crucial to protect global harvests and ensure high-quality food supplies. While priming is an established method in seed production for the fortification of plants against various stresses, it is not yet a standard practice in transplant cultivation. Thus, we evaluated the long-term effects of thermopriming—a heat-based priming technique—on the growth, development, and fruit yield of tomato plants. Following a recovery period of about six weeks for thermoprimed plants without stress inducers, we subjected them to subsequent salt stress to ascertain the persistence of the priming effects. Additionally, we compared the efficacy of thermopriming with benzothiadiazole (BTH), a chemical elicitor, in enhancing plant resilience to abiotic stress. While BTH application negatively impacted both plant growth and fruit health, thermopriming showed no such adverse effects on these parameters. Instead, thermopriming initially enhanced the plant defense mechanisms by increasing the accumulation of protective phenols and flavonoids in the leaves. Interestingly, while thermopriming did not alter the response to salt stress, it notably strengthened the overall resilience of the plants. Our findings underscore both the potential and temporal constraints of thermopriming memory. Nonetheless, primed plants exhibited temporarily increased stress tolerance, offering a means to safeguard the offspring.
Sex Dependence in Control of Renal Haemodynamics and Excretion in Streptozotocin Diabetic Rats—Role of Adenosine System and Nitric Oxide
Authors: Kuczeriszka Marta , Dobrowolski Leszek
Published: July 2024

Recently, we compared an interplay of the adenosine system and nitric oxide (NO) in the regulation of renal function between male normoglycaemic (NG) and streptozotocin-induced diabetic rats (DM). Considering the between-sex functional differences, e.g., in the NO status, we present similar studies performed in female rats. We examined if the theophylline effects (non-selective adenosine antagonist) in NG and DM females with or without active NO synthases differed from the earlier findings. In anaesthetised female Sprague Dawley rats, both NG and DM, untreated or after NO synthesis blockade with L-NAME, theophylline effects, on blood pressure, renal hemodynamics and excretion, and renal tissue NO were investigated. Renal artery blood flow (Transonic probe), cortical, outer-, and inner-medullary flows (laser-Doppler technique), and renal tissue NO signal (selective electrode) were measured. In contrast to males, in female NG and DM rats, theophylline induced renal vasodilation. In NO-deficient females, theophylline induced comparable renal vasodilatation, confirming the vasoconstrictor influence of the renal adenosine. In NG and DM females with intact NO synthesis, adenosine inhibition diminished kidney tissue NO, contrasting with an increase reported in males. Lowered baseline renal excretion in DM females suggested stimulation of renal tubular reabsorption due to the prevalence of antinatriuretic over natriuretic tubular action of adenosine receptors. An opposite inter-receptor balance pattern emerged previously from male studies. The study exposed between-sex functional differences in the interrelation of adenosine and NO in rats with normoglycaemia and streptozotocin diabetes. The findings also suggest that in diabetes mellitus, the abundance of individual receptor types can distinctly differ between females and males.
Examination of the Effect of Dimethyl Trisulfide in Acute Stress Mouse Model with the Potential Involvement of the TRPA1 Ion Channel
Authors: Göntér Kitti , Dombi Ágnes , Kormos Viktória , Pintér Erika , Pozsgai Gábor
Published: July 2024

Polysulfides are endogenously produced in mammals and generally associated with protective functions. Our aim was to investigate the effect of dimethyl trisulfide (DMTS) in a mouse model of acute stress. DMTS activates transient receptor potential ankyrin 1 (TRPA1) channels and leads to neuropeptide release, potentially that of substance P (SP). We hypothesize that DMTS might inhibit the degrading enzymes of endocannabinoids, so this system was also investigated as another possible pathway for mediating the effects of DMTS. Trpa1 gene wild-type (WT) and knockout (KO) mice were used to confirm the role of the TRPA1 ion channel in mediating the effects of DMTS. C57BL/6J, NK1 gene KO, and Tac1 gene KO mice were used to evaluate the effect of DMTS on the release and expression of SP. Some C57BL/6J animals were treated with AM251, an inhibitor of the cannabinoid CB1 receptor, to elucidate the role of the endocannabinoid system in these processes. Open field test (OFT) and forced swim test (FST) were performed in each mouse strain. A tail suspension test (TST) was performed in Trpa1 WT and KO animals. C-FOS immunohistochemistry was carried out on Trpa1 WT and KO animals. The DMTS treatment increased the number of highly active periods and decreased immobility time in the FST in WT animals, but had no effect on the Trpa1 KO mice. The DMTS administration induced neuronal activation in the Trpa1 WT mice in the stress-related brain areas, such as the locus coeruleus, dorsal raphe nucleus, lateral septum, paraventricular nucleus of the thalamus, and paraventricular nucleus of the hypothalamus. DMTS may have a potential role in the regulation of stress-related processes, and the TRPA1 ion channel may also be involved in mediating the effects of DMTS. DMTS can be an ideal candidate for further study as a potential remedy for stress-related disorders.
Functional Characterisation of Surfactant Protein A as a Novel Prophylactic Means against Oncogenic HPV Infections
Authors: Carse Sinead , Reid Tim , Madsen Jens , Clark Howard , Kirjakulov Artur , Bergant Marušič Martina , Schäfer Georgia
Published: July 2024

Human papillomavirus (HPV) infection poses a significant health challenge, particularly in low- and middle-income countries (LMIC), where limited healthcare access and awareness hinder vaccine accessibility. To identify alternative HPV targeting interventions, we previously reported on surfactant protein A (SP-A) as a novel molecule capable of recognising HPV16 pseudovirions (HPV16-PsVs) and reducing infection in a murine cervicovaginal HPV challenge model. Building on these findings, our current study aimed to assess SP-A’s suitability as a broad-spectrum HPV-targeting molecule and its impact on innate immune responses. We demonstrate SP-A’s ability to agglutinate and opsonise multiple oncogenic HPV-PsVs types, enhancing their uptake and clearance by RAW264.7 murine macrophages and THP-1 human-derived immune cells. The SP-A opsonisation of HPV not only led to increased lysosomal accumulation in macrophages and HaCaT keratinocytes but also resulted in a decreased infection of HaCaT cells, which was further decreased when co-cultured with innate immune cells. An analysis of human innate immune cell cytokine profiles revealed a significant inflammatory response upon SP-A exposure, potentially contributing to the overall inhibition of HPV infection. These results highlight the multi-layered impact of SP-A on HPV, innate immune cells and keratinocytes and lay the basis for the development of alternative prophylactic interventions against diverse HPV types.
Grape Seed Proanthocyanidin Extract Attenuates Cafeteria-Diet-Induced Liver Metabolic Disturbances in Rats: Influence of Photoperiod
Authors: Rodríguez Romina M. , Colom-Pellicer Marina , Hernández-Baixauli Julia , Calvo Enrique , Suárez Manuel , Arola-Arnal Anna , Torres-Fuentes Cristina , Aragonès Gerard , Mulero Miquel
Published: July 2024

This study investigated the influence of photoperiod (day length) on the efficacy of grape seed proanthocyanidin extract (GSPE) in mitigating metabolic disorders in obese rats fed a cafeteria diet. Rats were exposed to standard (L12), long (L18), or short (L6) photoperiods and treated with GSPE or vehicle. In the standard photoperiod, GSPE reduced body weight gain (50.5%), total cholesterol (37%), and triglycerides (34.8%), while increasing the expression of hepatic metabolic genes. In the long photoperiod, GSPE tended to decrease body weight gain, increased testosterone levels (68.3%), decreased liver weight (12.4%), and decreased reverse serum amino acids. In the short photoperiod, GSPE reduced glycemia (~10%) and lowered triglyceride levels (38.5%), with effects modified by diet. The standard photoperiod showed the greatest efficacy against metabolic syndrome-associated diseases. The study showed how day length affects GSPE’s benefits and underscores considering biological rhythms in metabolic disease therapies.
Mesenchymal Stem Cell-Derived Exosomes as Drug Delivery Vehicles in Disease Therapy
Authors: Zhao Wenzhe , Li Kaixuan , Li Liangbo , Wang Ruichen , Lei Yang , Yang Hui , Sun Leming
Published: July 2024

Exosomes are small vesicles containing proteins, nucleic acids, and biological lipids, which are responsible for intercellular communication. Studies have shown that exosomes can be utilized as effective drug delivery vehicles to accurately deliver therapeutic substances to target tissues, enhancing therapeutic effects and reducing side effects. Mesenchymal stem cells (MSCs) are a class of stem cells widely used for tissue engineering, regenerative medicine, and immunotherapy. Exosomes derived from MSCs have special immunomodulatory functions, low immunogenicity, the ability to penetrate tumor tissues, and high yield, which are expected to be engineered into efficient drug delivery systems. Despite the promising promise of MSC-derived exosomes, exploring their optimal preparation methods, drug-loading modalities, and therapeutic potential remains challenging. Therefore, this article reviews the related characteristics, preparation methods, application, and potential risks of MSC-derived exosomes as drug delivery systems in order to find potential therapeutic breakthroughs.
Therapeutic Effect of Lecigel, Cetiol®CC, Activonol-6, Activonol-M, 1,3-Propanediol, Soline, and Fucocert® (LCAA-PSF) Treatment on Imiquimod-Induced Psoriasis-like Skin in Mice
Authors: Li Chih-Ching , Lin Chih-Chien , Lee Chun-Yi , Sheu Meei-Ling , Tsai Yi-Ching , Tsai Chia-Yun , Wu Hao-Ting , Wu Ren-Jang , Lai De-Wei
Published: July 2024

The individual ingredients of 1,3-Propanediol, Soline, and Fucocert® (PSF) are often used as cosmetic formulations in skin care. In addition, the mixture of Lecigel, Cetiol®CC, Activonol-6, and Activonol-M (LCAA) is often used as a cosmetic base. However, whether the combination of LCAA with PSF (LCAA-PSF) exerts a therapeutic effect on psoriasis remains unclear. In this study, mice induced with imiquimod (IMQ) were divided into three groups and administered 100 mg/day of LCAA, 100 mg/day of LCAA-PSF, or Vaseline on the dorsal skin of each mouse. Weight-matched mice treated with Vaseline alone were used as controls. Hematoxylin and eosin (H&E) staining and enzyme-linked immunosorbent assay(ELISA) were used to assess tissue morphology and inflammatory cytokines. RNA sequencing analysis was used to predict the mechanism underlying the action of LCAA-PSF against psoriasis, while immunohistochemical analysis validation was used to identify pertinent molecular pathways. The results demonstrated that LCAA-PSF alleviated IMQ-induced keratinocyte differentiation/ proliferation bydecreasingthe serum levels of inflammatory cytokines such as IL-6, TNF-α, IL-23, and IL-17A and the epidermisof TGFβ, Ki67, CK5/6, and VEGF expression, which is associated with angiogenesis and keratinocyte differentiation/ proliferation. These findings highlight the antipsoriatic activity of LCAA-PSF in a psoriasis-like mouse model and suggest this may occurvia the inhibition of inflammatory factor secretionand the TGFβ-related signal pathway.
Exploring the Role of PD-1 in the Autoimmune Response: Insights into Its Implication in Systemic Lupus Erythematosus
Authors: Sagrero-Fabela Nefertari , Chávez-Mireles Ramón , Salazar-Camarena Diana Celeste , Palafox-Sánchez Claudia Azucena
Published: July 2024

Despite advances in understanding systemic lupus erythematosus (SLE), many challenges remain in unraveling the precise mechanisms behind the disease’s development and progression. Recent evidence has questioned the role of programmed cell death protein 1 (PD-1) in suppressing autoreactive CD4+ T cells during autoimmune responses. Research has investigated the potential impacts of PD-1 on various CD4+ T-cell subpopulations, including T follicular helper (Tfh) cells, circulating Tfh (cTfh) cells, and T peripheral helper (Tph) cells, all of which exhibit substantial PD-1 expression and are closely related to several autoimmune disorders, including SLE. This review highlights the complex role of PD-1 in autoimmunity and emphasizes the imperative for further research to elucidate its functions during autoreactive T-cell responses. Additionally, we address the potential of PD-1 and its ligands as possible therapeutic targets in SLE.
Potential Application of the Myocardial Scintigraphy Agent [123I]BMIPP in Colon Cancer Cell Imaging
Authors: Sato Kakeru , Hirayama Yuka , Mizutani Asuka , Yao Jianwei , Higashino Jinya , Kamitaka Yuto , Muranaka Yuka , Yamazaki Kana , Nishii Ryuichi , Kobayashi Masato , Kawai Keiichi
Published: July 2024

[123I]β-methyl-p-iodophenyl-pentadecanoic acid ([123I]BMIPP), which is used for nuclear medicine imaging of myocardial fatty acid metabolism, accumulates in cancer cells. However, the mechanism of accumulation remains unknown. Therefore, this study aimed to elucidate the accumulation and accumulation mechanism of [123I]BMIPP in cancer cells. We compared the accumulation of [123I]BMIPP in cancer cells with that of [18F]FDG and found that [123I]BMIPP was a much higher accumulation than [18F]FDG. The accumulation of [123I]BMIPP was evaluated in the presence of sulfosuccinimidyl oleate (SSO), a CD36 inhibitor, and lipofermata, a fatty acid transport protein (FATP) inhibitor, under low-temperature conditions and in the presence of etomoxir, a carnitine palmitoyl transferase I (CPT1) inhibitor. The results showed that [123I]BMIPP accumulation was decreased in the presence of SSO and lipofermata in H441, LS180, and DLD-1 cells, suggesting that FATPs and CD36 are involved in [123I]BMIPP uptake in cancer cells. [123I]BMIPP accumulation in all cancer cell lines was significantly decreased at 4 °C compared to that at 37 °C and increased in the presence of etomoxir in all cancer cell lines, suggesting that the accumulation of [123I]BMIPP in cancer cells is metabolically dependent. In a biological distribution study conducted using tumor-bearing mice transplanted with LS180 cells, [123I]BMIPP highly accumulated in not only LS180 cells but also normal tissues and organs (including blood and muscle). The tumor-to-intestine or large intestine ratios of [123I]BMIPP were similar to those of [18F]FDG, and the tumor-to-large-intestine ratios exceeded 1.0 during 30 min after [123I]BMIPP administration in the in vivo study. [123I]BMIPP is taken up by cancer cells via CD36 and FATP and incorporated into mitochondria via CPT1. Therefore, [123I]BMIPP may be useful for imaging cancers with activated fatty acid metabolism, such as colon cancer. However, the development of novel imaging radiotracers based on the chemical structure analog of [123I]BMIPP is needed.