bluuebunny/crossref_metadata_2025 huggingface dataset:
Short-Term Solar Power Predicting Model Based on Multi-Step CNN Stacked LSTM Technique
Authors: Elizabeth Michael Neethu , Mishra Manohar , Hasan Shazia , Al-Durra Ahmed
Published: March 2022

Variability in solar irradiance has an impact on the stability of solar systems and the grid’s safety. With the decreasing cost of solar panels and recent advancements in energy conversion technology, precise solar energy forecasting is critical for energy system integration. Despite extensive research, there is still potential for advancement of solar irradiance prediction accuracy, especially global horizontal irradiance. Global Horizontal Irradiance (GHI) (unit: KWh/m2) and the Plane Of Array (POA) irradiance (unit: W/m2) were used as the forecasting objectives in this research, and a hybrid short-term solar irradiance prediction model called modified multi-step Convolutional Neural Network (CNN)-stacked Long-Short-Term-Memory network (LSTM) with drop-out was proposed. The real solar data from Sweihan Photovoltaic Independent Power Project in Abu Dhabi, UAE is preprocessed, and features were extracted using modified CNN layers. The output result from CNN is used to predict the targets using a stacked LSTM network and the efficiency is proved by comparing statistical performance measures in terms of Root Mean Square Error (RMSE), Mean Absolute Percentage Error (MAPE), Mean Squared Error (MAE), and R2 scores, with other contemporary machine learning and deep-learning-based models. The proposed model offered the best RMSE and R2 values of 0.36 and 0.98 for solar irradiance prediction and 61.24 with R2 0.96 for POA prediction, which also showed better performance as compared to the published works in the literature.
Abnormal Data Detection and Identification Method of Distribution Internet of Things Monitoring Terminal Based on Spatiotemporal Correlation
Authors: Shao Nan , Chen Yu
Published: March 2022

As an important part of the ubiquitous power Internet of Things, the distribution Internet of Things can further improve the automation and informatization level of the distribution network. The reliability of the measurement data of the low-voltage terminal unit, as the sensing unit of the sensing layer of the distribution Internet of Things, has a great impact on the fault processing and advanced applications of the distribution Internet of Things. The self-check and the equipment working status monitoring of the main station of the low-voltage terminal unit struggle to identify the abnormality of measurement data. Aiming at this problem, an abnormal data detection and identification recognition method of a distribution Internet of Things monitoring terminal is proposed on the basis of spatiotemporal correlation. First, using the temporal correlation of monitoring terminal data, the proposed composite temporal series similarity measurement criterion is used to calculate the distance matrix between data, and the abnormal data detection is realized via combination with the improved DBSCAN algorithm. Then, using the spatial correlation of the data of the terminal unit, the geometric features of the spatial cross-correlation coefficient of the terminal nodes are extracted as the input of the cascaded fuzzy logic system to identify the abnormal source. Lastly, the effectiveness of the method is verified by a practical example.
Comparison of Characteristics of Poultry Litter Pellets Obtained by the Processes of Dry and Wet Torrefaction
Authors: Isemin Rafail , Mikhalev Alexander , Milovanov Oleg , Klimov Dmitry , Kokh-Tatarenko Vadim , Brulé Mathieu , Tabet Fouzi , Nebyvaev Artemy , Kuzmin Sergey , Konyakhin Valentin
Published: March 2022

Torrefaction is a technology for the preliminary thermochemical treatment of biomass in order to improve its fuel characteristics. The aim of this work is to conduct comparative studies and select the optimal operating conditions of fluidized bed torrefaction for the processing of poultry litter (PL) into an environmentally friendly fuel. PL torrefaction was evaluated according to three different process configurations: (1) torrefaction of PL pellets in a fixed bed in a nitrogen medium at temperatures of 250 °C, 300 °C and 350 °C (NT1, NT2 and NT3); (2) torrefaction of PL pellets in a fluidized bed of quartz sand in a nitrogen medium at temperatures of 250 °C, 300 °C and 350 °C (NT4, NT5 and NT6); and (3) torrefaction of PL pellets in a fluidized bed of quartz sand in an environment of superheated steam at temperatures of 250 °C, 300 °C and 350 °C (ST1, ST2 and ST3). The duration of the torrefaction process in all experiments was determined by the time required for completion of CO2, CO, H2, and CH4 release from the treated biomass samples. The gas analyzer (Vario Plus Syngaz) was used to measure the concentration of these gases. The torrefaction process began from the moment of loading the PL sample into the reactor, which was heated to the required temperature. After the start of the torrefaction process, the concentration of CO2, CO, H2, and CH4 in the gases leaving the reactor initially increased and, subsequently, dropped sharply, indicating the completion of the torrefaction process. The chemical composition of the obtained biochar was studied, and it was found that the biochar contained approximately equal amounts of oxygen, carbon, nitrogen, hydrogen and ash, regardless of the torrefaction method. Furthermore, the biogas yield of the liquid condensate, obtained from the cooling of superheated steam used in the torrefaction process, was evaluated. The results highlight the efficiency of fluidized bed torrefaction, as well as the performance of superheated steam as a fluidization medium.
A Permanent Magnet Assembling Approach to Mitigate the Cogging Torque for Permanent Magnet Machines Considering Manufacturing Uncertainties
Authors: Liu Haipeng , Jin Xin , Bianchi Nicola , Bramerdorfer Gerd , Hu Pengzhong , Zhang Chengning , Yang Yongxi
Published: March 2022

Conventionally, the small mean and variance of peak-to-peak cogging torque of permanent magnet (PM) machines considering manufacturing uncertainties can be achieved by a robust design or by reducing the uncertainties range. However, the consequent compromise of other design objectives or the increase in the manufacturing costs are frequently inevitable. In this paper, the combination sequence of the uncertainties is highlighted and implemented to achieve a stable performance even for a non-robust design without increasing the cost too much. A PM assembling approach is proposed to mitigate the influences of PM uncertainties on cogging torque by means of combining the uncertainties in a particular sequence, where the effects of uncertainties would counteract each other. Both the singular type of PM uncertainties and the combined ones are considered in the assembling approach. Furthermore, the proposed approach is verified by comparing the cogging torque performance with PM randomly assembled models, where several hundreds of models featuring different uncertainties are calculated through the finite element method. The proposed approach is discussed, particularly for the application of mass production and a small amount of prototypes. Two prototypes with different PM assembling sequences are fabricated and further verify the effectiveness of the proposed PM assembling approach.
A Nash Equilibrium Approach to the Brazilian Seasonalization of Energy Certificates
Authors: dos Santos Fellipe Fernandes Goulart , de Castro Lobato Marcus Vinícius , Vieira Douglas Alexandre Gomes , Lisboa Adriano Chaves , Saldanha Rodney Rezende
Published: March 2022

This paper presents a Nash equilibrium approach to model a non-cooperative game that takes place among Brazilian hydro-generating companies in the annual process called “seasonalization”. We have “remade” the seasonalizations that occurred in the period of 2013–2020 by using our model and comparing the financial outcomes with the current ones that resulted from the seasonalizations the generators made each year, with financial improvements in almost every year. By using the Nash equilibrium approach, it is possible to achieve optimal decisions concerning the seasonalization process that were not evident by using traditional methods. This approach is useful for companies willing to enhance their income and to improve their risk management by making better choices in seasonalization.
Current State and Future Direction for Building Resilient Water Resources and Infrastructure Systems
Authors: Khatri Krishna B.
Published: March 2022

Planning and developing resilient socio-technical and natural systems to cope with and respond to unprecedented changes has been one of the top goals of government bodies, researchers, and practitioners worldwide. This study aims to review how resilience is defined and evaluated in water resources and infrastructure systems (hereafter water systems) and propose a framework to analyze and incorporate resilience in the system. Two questions guide the review: How is resilience defined in water systems compared to other disciplines? What are commonly used resilience measures and methods applicable to water systems? Based on the review, a resilience analysis framework has been proposed. The framework uses a system of systems approach and applies hierarchical holographic modeling to address the complexity of interdependent systems. The resilience of the systems was analyzed using three questions: resilience of what, resilience to what, and resilience for whom. Two resilience measures selected for the analysis are robustness and rapidity. The framework also includes methods for uncertainty analysis, options for resilience strategies, and multi-criteria decision analysis methods to select optimal resilience options. The review is not exhaustive due to the broader topic but aims to present necessary background information to support the proposed framework.
Efficacy of Plant Sterol-Enriched Food for Primary Prevention and Treatment of Hypercholesterolemia: A Systematic Literature Review
Authors: Turini Elisa , Sarsale Miriana , Petri Davide , Totaro Michele , Lucenteforte Ersilia , Tavoschi Lara , Baggiani Angelo
Published: March 2022

Plant sterols/phytosterols (PSs) are molecules with a similar structure to cholesterol that have a recognized effect on elevated LDL concentrations (LDL-c). PSs are used as a natural therapy against elevated LDL-c in combination with a healthy diet and exercise. A systematic review was performed to evaluate the efficacy of PS-enriched foods in the treatment of hypercholesterolemia. Randomized controlled clinical studies reporting the use of PS-enriched foods to reduce LDL-c among adult individuals were retrieved and assessed for risk of bias. Meta-analyses were performed to assess changes in LDL-c by treatment, food matrix, LDL-c range, sterols dosage and risk of bias (RoB). In the 13 studies analyzed, LDL-c in PS-treated participants decreased by an average of 12.14 (8.98; 15.29) mg/dL. PS administration was statistically more effective in patients with LDL-c ≥ 140 mg/dL and for PS dosages > 2 g/day. It can be concluded that PSs can be used as an important primary prevention measure for hypercholesterolemia and as tertiary prevention for cardiovascular events in patients who already have mild to moderate LDL-c. However, in severe hypercholesterolemia and in cases of familial hypercholesterolemia, it is necessary to combine dietary treatment with the use of statins.
The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.)
Authors: Zhang Weida , Guo Minrui , Yang Wanting , Liu Yuxing , Wang Yue , Chen Guogang
Published: March 2022

To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties (‘Xizhoumi 17’, ‘Jinhuami 25’, and ‘Chougua’) representing three different storability levels. The results showed that ‘Chougua’ maintained a higher firmness on day 18, with the lowest decay incidence (0%). ‘Chougua’ showed a better storage quality and intact cell wall structure. The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons. Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage. There were similar softening mechanisms in the three Hami melons. This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
Mechanochemical Effect on Controlled Drug Release of Konjac Glucomannan Matrix Tablets during Dry Grinding
Authors: Okazaki Fuminori , Hattori Yusuke , Sasaki Tetsuo , Otsuka Makoto
Published: March 2022

To design a controlled drug release preparation based on a safe natural material, a Konjac glucomannan (KGM) mixture containing 16.0 w/w% calcium hydroxide (Ca(OH)2) was ground in a planetary ball mill for 0–120 min. The mechanochemical effect on the physicochemical properties of the KGM ground product was investigated by Fourier-transform infrared spectroscopy (FT-IR), powder X-ray spectroscopy, scanning electron microscopy with energy-dispersive X-ray spectroscopy, and drug release testing. The FT-IR spectra of the ground KGM indicated that the deacetylation reaction of KGM was accelerated in the Ca(OH)2-containing sols by mechanochemical energy, and the degree of deacetylation of KGM was dependent on the grinding time. The time required for tablet disintegration of the KGM matrix tablets containing theophylline increased as the grinding time increased; therefore, drug release was sustained. The Higuchi plots of the matrix tablets obtained from KGM ground for 60–120 min exhibited good linearity because they maintained their gel matrix tablet shape during the release test. However, KGM tablets ground for 0–30 min exhibited nonlinear curves, which were caused by tablet disintegration. This suggests that drug release from the KGM matrix tablet can be freely controlled by the degree of mechanochemical treatment.
High Resistance to Quinclorac in Multiple-Resistant Echinochloa colona Associated with Elevated Stress Tolerance Gene Expression and Enriched Xenobiotic Detoxification Pathway
Authors: Rangani Gulab , Rouse Christopher E. , Saski Christopher , Noorai Rooksana E. , Shankar Vijay , Lawton-Rauh Amy L. , Werle Isabel S. , Roma-Burgos Nilda
Published: March 2022

Echinochloa colona and other species in this genus are a threat to global rice production and food security. Quinclorac, an auxin mimic, is a common herbicide for grass weed control in rice, and Echinochloa spp. have evolved resistance to it. The complete mode of quinclorac action and subsequent evolution of resistance is not fully understood. We analyzed the de novo transcriptome of multiple-herbicide-resistant (ECO-R) and herbicide-susceptible genotypes in response to quinclorac. Several biological processes were constitutively upregulated in ECO-R, including carbon metabolism, photosynthesis, and ureide metabolism, indicating improved metabolic efficiency. The transcriptional change in ECO-R following quinclorac treatment indicates an efficient response, with upregulation of trehalose biosynthesis, which is also known for abiotic stress mitigation. Detoxification-related genes were induced in ECO-R, mainly the UDP-glycosyltransferase (UGT) family, most likely enhancing quinclorac metabolism. The transcriptome data also revealed that many antioxidant defense elements were uniquely elevated in ECO-R to protect against the auxin-mediated oxidative stress. We propose that upon quinclorac treatment, ECO-R detoxifies quinclorac utilizing UGT genes, which modify quinclorac using the sufficient supply of UDP-glucose from the elevated trehalose pathway. Thus, we present the first report of upregulation of trehalose synthesis and its association with the herbicide detoxification pathway as an adaptive mechanism to herbicide stress in Echinochloa, resulting in high resistance.
Global Health Governance and Health Equity in the Context of COVID-19: A Scoping Review
Authors: Kheir-Mataria Wafa Abu El , El-Fawal Hassan , Bhuiyan Shahjahan , Chun Sungsoo
Published: March 2022

Background: Health equity is an important aspect of responsible governance. COVID-19 exposed existing shortfalls of Global Health Governance (GHG). A considerable amount of related literature is produced. This scoping review aims at mapping the present knowledge and at identifying research gaps. Methods: This scoping review is based on the Joanna Briggs Institute’s guideline for standardized methods and PRISMA-ScR guidelines for reporting. Documents published from December 2019 to October 2021 were searched using PubMed, Scopus, Google Scholar, World cat, and WHO-Global Index Medicus. Two reviewers screened and reviewed eligible studies in three stages: duplicates identification and elimination, title and abstract screening, and full-text assessment. Data was charted and results were classified into conceptual categories. Analysis was done in three stages: open descriptive coding, focused thematic analysis, and frequency, commonality and significance analysis. Results: forty-nine studies met the inclusion criteria. Areas of research were grouped into seven themes: “human rights and inequities”, “solidarity, collaboration and partnership”, “GHG structure change”, “political and economic power and finance”, “approaches to address inequity”, “law and regulations”, and “private investment and public-private partnerships (PPPs) in GHG”. The highest number of papers were in the first theme, “human rights and inequities”. However, the themes are interrelated. Authors who contributed to research were mostly affiliated to developed countries indicating a gap in knowledge and expertise in developing countries. Conclusion: Through this scoping review we found that the seven themes are interconnected. Disciplinary collaboration in research relating GHG to health inequities is solicited. Collaboration in research, information sharing, and research capacity development are in needed in developing countries.
Resistance Management through Brassica Crop–TuMV–Aphid Interactions: Retrospect and Prospects
Authors: Lu Xinxin , Huang Wenyue , Zhang Shifan , Li Fei , Zhang Hui , Sun Rifei , Li Guoliang , Zhang Shujiang
Published: March 2022

Turnip mosaic virus (TuMV) is an important threat to the yield and quality of brassica crops in China, and has brought serious losses to brassica crops in the Far East, including China and the north. Aphids (Hemiptera, Aphidoidea) are the main mediators of TuMV transmission in field production, and not only have strong virus transmission ability (small individuals, strong concealment, and strong fecundity), but are also influenced by the environment, making them difficult to control. Till now, there have been few studies on the resistance to aphids in brassica crops, which depended mainly on pesticide control in agriculture production. However, the control effect was temporarily effective, which also brought environmental pollution, pesticide residues in food products, and destroyed the ecological balance. This study reviews the relationship among brassica crop–TuMV, TuMV–aphid, and brassica crop–aphid interactions, and reveals the influence factors (light, temperature, and CO2 concentration) on brassica crop–TuMV–aphid interactions, summarizing the current research status and main scientific problems about brassica crop–TuMV–aphid interactions. It may provide theoretical guidance for opening up new ways of aphid and TuMV management in brassica crops.
Social Distancing and Isolation Strategies to Prevent and Control the Transmission of COVID-19 and Other Infectious Diseases in Care Homes for Older People: An International Review
Authors: Sims Sarah , Harris Ruth , Hussein Shereen , Rafferty Anne Marie , Desai Amit , Palmer Sinead , Brearley Sally , Adams Richard , Rees Lindsay , Fitzpatrick Joanne M.
Published: March 2022

Older people living in care homes are at high risk of poor health outcomes and mortality if they contract COVID-19 or other infectious diseases. Measures used to protect residents include social distancing and isolation, although implementation is challenging. This review aimed to assess the social distancing and isolation strategies used by care homes to prevent and control the transmission of COVID-19 and other infectious diseases. Seven electronic databases were searched: Medline, CINAHL, Embase, PsycINFO, HMIC, Social Care Online, and Web of Science Core Collection. Grey literature was searched using MedRxiv, PDQ-Evidence, NICE Evidence Search, LTCCovid19.org and TRIP. Extracted data were synthesised using narrative synthesis and tabulation. 103 papers were included (10 empirical studies, seven literature reviews, and 86 policy documents). Strategies used to prevent and control the transmission of COVID-19 and other infectious diseases included social distancing and isolation of residents and staff, zoning and cohorting of residents, restriction of resident movement/activities, restriction of visitors and restriction of staff working patterns. This review demonstrates a lack of empirical evidence and the limited nature of policy documentation around social distancing and isolation measures in care homes. Evaluative research on these interventions is needed urgently, focusing on the well-being of all residents, particularly those with hearing, vision or cognitive impairments.
Forest Bathing Is Better than Walking in Urban Park: Comparison of Cardiac and Vascular Function between Urban and Forest Parks
Authors: Tsao Tsung-Ming , Hwang Jing-Shiang , Lin Sung-Tsun , Wu Charlene , Tsai Ming-Jer , Su Ta-Chen
Published: March 2022

Forest bathing is beneficial for human health. To investigate whether walking in forest or urban parks affects cardiovascular functions (CVFs), the present study was conducted in five forest trails in the Xitou Experimental Forest and in five urban parks in Taipei city. We recruited 25 adult volunteers for an observational pilot study in forest parks (n = 14) and urban parks (n = 11). CVFs were assessed by measuring the arterial pressure waveform using an oscillometric blood pressure (BP) device. The baseline and paired differences of systolic BP (SBP), central end SBP, heart rate, left ventricle (LV) dP/dt max and cardiac output in participants were lower before and after walking in a forest park than those in an urban park. In addition, the systemic vascular compliance and brachial artery compliance of those who walked in a forest park were significantly higher compared with those in an urban park. Linear mixed models demonstrated lower levels of SBP by 5.22 mmHg, heart rate by 2.46 beats/min, and cardiac output by 0.52 L/min, and LV dP/dt max by 146.91 mmHg/s among those who walked in forest compared to those in an urban park after controlling covariates. This study provides evidence of the potential beneficial effects of walking exercise in forest parks on CVFs.
An Empirical Study of the Restoration Potential of Urban Deciduous Forest Space to Youth
Authors: Wu Linjia , Dong Qidi , Luo Shixian , Li Yanling , Liu Yuzhou , Li Jiani , Zhu Zhixian , He Mingliang , Luo Yuhang , Chen Qibing
Published: March 2022

Urban deciduous forests are an important ecological resource and seasonal landscape in the urban environment. However, in the abundant literature on how urban green space promotes human health and well-being, research on urban seasonal deciduous forests is limited. This study aimed to investigate the physiological and psychological recovery potential provided of urban deciduous forest space for youths and the spatial preferences of youths regarding such spaces. We recruited 120 participants to study the restorative potential of two typical urban deciduous forest landscape spaces (experimental groups) and one urban road environment (control group). The results showed that after 15 min of observation, the blood pressure (especially the diastolic blood pressure (p < 0.01)) and pulse of the deciduous forest trail setting (DFTS) group effectively decreased, and the restorative mood significantly increased. Regarding change in emotional parameters, the DFTS group scored higher on “interest” and significantly higher than the other two groups on positive emotion. The correlation results show that density and level are the key factors affecting spatial preferences regarding complex deciduous forests. An increase in density reduces the mood of re-laxation, and an increase in level decreases fatigue and interest. We suggest (1) constructing foot-paths in urban deciduous forests to reduce their spatial density as to improve the relaxation effect and (2) increasing landscape diversity according to the forest space to facilitate user participation and interest. This study provides a scientific basis for the environmental restoration of deciduous landscapes and for urban forestry management decision-makers based on space type construction.
Does Treatment Readiness Shape Service-Design Preferences of Gay, Bisexual, and Other Men Who Have Sex with Men Who Use Crystal Methamphetamine? A Cross Sectional Study
Authors: Card Kiffer G. , McGuire Madison , Berlin Graham W. , Wells Gordon A. , Fulcher Karyn , Nguyen Tribesty , Hart Trevor A. , Skakoon Sparling Shayna , Lachowsky Nathan J.
Published: March 2022

Crystal methamphetamine (CM) disproportionately impacts gay, bisexual, and other men who have sex with men (gbMSM). However, not all gbMSM are interested in changing their substance use. The present study aimed to examine whether participant-preferred service characteristics were associated with their readiness to change. We surveyed gbMSM who used CM in the past six months, aged 18 plus years, on dating platforms. Participants rated service-design characteristics from “very unimportant” to “very important”. Multivariable regression tested service preference ratings across levels of the Stages of Change Readiness and Treatment Eagerness Scale (SOCRATES-8D). Among 291 participants, 38.7% reported their CM use was not problematic, 19.5% were not ready to take any action to reduce or stop using CM, and 41.7% were ready to take action. On average, participants rated inclusive, culturally-appropriate, out-patient counselling-based interventions as most important. Participants with greater readiness-to-change scores rated characteristics higher than gbMSM with lesser readiness. Contingency management and non-abstinence programming were identified as characteristics that might engage those with lesser readiness. Services should account for differences in readiness-to-change. Programs that provide incentives and employ harm reduction principles are needed for individuals who may not be seeking to reduce or change their CM use.
Testing the Feasibility and Potential Impact of a Mindfulness-Based Pilot Program in Urban School Youth
Authors: Krebs Sabrina , Moak Emily , Muhammadi Shakiba , Forbes David , Yeh Ming-Chin , Leung May May
Published: March 2022

Mindfulness-based interventions (MBIs) could be effective in engaging children and reducing childhood obesity risk. The purpose of this study was to test feasibility, fidelity, and potential impact of a pilot MBI in urban school youth. A two-group quasi-experimental study was conducted in a Harlem, New York school. Participants comprised 51 students (ages 9–12, 54% female, 85% African American/Black). The experimental (E) group (n = 26) participated in a nine-session pilot MBI. Sessions were 90 min and offered weekly as part of afterschool programming. Children only attending during the school day comprised the control (C) group (n = 25). Process evaluation (e.g., fidelity, reach) was performed. Interviews with the E group were conducted to determine program acceptability. Mindful eating and resilience measures were collected at baseline and post-intervention. Intervention feasibility was high as the retention rate was 100% and fidelity was good as nine out of ten sessions were implemented. Relative to baseline, significant improvements were observed in the C group compared to the E group in the resilience composite score (p = 0.01) and its confidence domain (p = 0.01). A MBI may provide a unique opportunity to engage youth. However, further research is warranted to determine if a MBI could promote health in urban, school-age children.
Avoidable Mortality between Metropolitan and Non-Metropolitan Areas in Korea from 1995 to 2019: A Descriptive Study of Implications for the National Healthcare Policy
Authors: Choi Min-Hyeok , Moon Min-Hui , Yoon Tae-Ho
Published: March 2022

This study aims to investigate the trends of avoidable mortality and regional inequality from 1995 to 2019 and to provide evidence for policy effectiveness to address regional health disparities in Korea. Mortality and population data were obtained from the Statistics Korea database. Age-standardized all-cause, avoidable, preventable, and treatable mortality was calculated for each year by sex and region. Changes in mortality trends between metropolitan and non-metropolitan areas were compared with absolute and relative differences. Avoidable mortality decreased by 65.7% (350.5 to 120.2/100,000 persons) in Korea, 64.5% in metropolitan areas, and 65.8% in non-metropolitan areas. The reduction in avoidable mortality was greater in males than in females in both areas. The main causes of death that contribute to the reduction of avoidable mortality are cardiovascular diseases, cancer, and injuries. In preventable mortality, the decrease in non-metropolitan areas (−192.4/100,000 persons) was greater than that in metropolitan areas (−142.7/100,000 persons). However, in treatable mortality, there was no significant difference between the two areas. While inequalities in preventable mortality improved, inequalities in treatable mortality worsened, especially in females. Our findings suggest that regional health disparities can be resolved through a balanced regional development strategy with an ultimate goal of reducing health disparities.
Trends in Use of Alcohol and Cigarettes among Danish Adolescents, 2002–2018: Exclusive and Dual Use
Authors: Kjeld Simone G. , Lund Lisbeth , Madsen Katrine R. , Damsgaard Mogens T. , Bast Lotus S.
Published: March 2022

Many young adolescents experiment with substance use which can have substantial health implications later in life. This study examined trends in substance use among Danish adolescents from 2002 to 2018, including exclusive and dual current use of alcohol and cigarettes. Data on 13- and 15-year-olds (N = 15,295) from five comparable cross-sectional Health Behavior in School-aged Children (HBSC) surveys were used. Cochran-Armitage test for trend assessed the development in substance use patterns over time. Overall, a decreasing trend in current use of alcohol and cigarettes was found among Danish adolescents during the 16-year study period: from 71.7% in 2002 to 51.6% in 2018. In 2018, most adolescents (41.8%) currently used alcohol exclusively, 8.6% had a dual current use of cigarettes and alcohol, and 1.3% smoked cigarettes exclusively. Trends in alcohol use differed according to age groups, while no gender-specific trends in substance use were found. Findings suggest that a significant prevention potential in adolescent substance use remains, and future initiatives may focus on dual use of substances as well as tailored efforts to specific subgroups in high risk of using substances.
COVID-19 Pandemic in Portugal: Psychosocial and Health-Related Factors Associated with Psychological Discomfort
Authors: Pais-Ribeiro José , Ferreira-Valente Alexandra , Jarego Margarida , Sánchez-Rodríguez Elisabet , Miró Jordi
Published: March 2022

The COVID-19 pandemic is a stressful long-lasting event with an increasingly negative impact upon individuals. This study aimed at assessing the magnitude of depression, anxiety, and stress among adults living in Portugal during the first mandatory lockdown of 2020, and the psychosocial and health-related factors associated with these symptoms. A sample of 484 adults (73% women) with an average age of 40 years old (Standard Deviation, SD = 14.03) responded to an online survey. The survey included measures of depression, anxiety, stress, social support, COVID-19 interference in daily life, attitudes towards COVID-19, and health perception. The impact of the lockdown on psychological well-being was large, with up to 36% of the participants showing signs of at least mild psychological discomfort (i.e., depression, anxiety, and stress). Social support, COVID-19 interference on daily life, health perception, and age, explained all the dependent variables. Education level, income, attitudes towards COVID-19, and gender explained some of the dependent variables. These results suggest that the COVID-19 pandemic has a serious impact on the psychological health of Portuguese adults. The role of the procedures to control the pandemic on the mental health of Portuguese adults should not be underestimated.
Multi-Criterial Algorithm for the Efficient and Ergonomic Manual Assembly Process
Authors: Turk Maja , Šimic Marko , Pipan Miha , Herakovič Niko
Published: March 2022

Industry 4.0 introduces smart solutions throughout the company’s supply chain, including manual assembly, where the goal is to ensure shorter work cycle time, increase productivity and quality, while minimizing costs. Following the principles of this paradigm, this paper proposes a digital transformation of the manual assembly process by implementing a multi-criterial algorithm (MCA) for adjusting and configuring a human-centered smart manual assembly workstation to ensure efficient and ergonomic performance of the manual assembly process. The MCA takes into account various influential parameters, such as the anthropometry of the individual worker, gender, complexity of the assembly process, product characteristics, and product structure. The efficiency of the MCA was verified both in the laboratory environment with the time analysis and in the virtual environment using Digital Human Modelling through several ergonomic analyses. The results of the implementation of the MCA on a manual assembly workstation support the digital (re)design of the manual assembly process with the aim of creating an efficient and ergonomically suitable workstation for each worker, thus increasing the productivity and efficiency of the human-centered manual assembly process.
A Novel Sprague-Dawley Rat Model Presents Improved NASH/NAFLD Symptoms with PEG Coated Vitexin Liposomes
Authors: Farooq Adil , Iqbal Arfa , Rana Nosheen Fatima , Fatima Misha , Maryam Tuba , Batool Farhat , Rehman Zahra , Menaa Farid , Azhar Shabia , Nawaz Afrah , Amin Faheem , Mohammedsaleh Zuhair M. , Alrdahe Salma Saleh
Published: March 2022

Chronic liver disease (CLD) is a global threat to the human population, with manifestations resulting from alcohol-related liver disease (ALD) and non-alcohol fatty liver disease (NAFLD). NAFLD, if not treated, may progress to non-alcoholic steatohepatitis (NASH). Furthermore, inflammation leads to liver fibrosis, cirrhosis, and hepatocellular carcinoma. Vitexin, a natural flavonoid, has been recently reported for inhibiting NAFLD. It is a lipogenesis inhibitor and activates lipolysis and fatty acid oxidation. In addition, owing to its antioxidant properties, it appeared as a hepatoprotective candidate. However, it exhibits low bioavailability and low efficacy due to its hydrophobic nature. A novel rat model for liver cirrhosis was developed by CCL4/Urethane co-administration. Vitexin encapsulated liposomes were synthesized by the ‘thin-film hydration’ method. Polyethylene glycol (PEG) was coated on liposomes to enhance stability and stealth effect. The diseased rats were then treated with vitexin and PEGylated vitexin liposomes, administered intravenously and orally. Results ascertained the liposomal encapsulation of vitexin and subsequent PEG coating to be a substantial strategy for treating liver cirrhosis through oral drug delivery.
Specialized Proresolving Lipid Mediators: A Potential Therapeutic Target for Atherosclerosis
Authors: Salazar Juan , Pirela Daniela , Nava Manuel , Castro Ana , Angarita Lissé , Parra Heliana , Durán-Agüero Samuel , Rojas-Gómez Diana Marcela , Galbán Néstor , Añez Roberto , Chacín Maricarmen , Diaz Andrea , Villasmil Nelson , De Sanctis Juan Bautista , Bermúdez Valmore
Published: March 2022

Cardiovascular disease (CVD) is a global public health issue due to its high morbidity, mortality, and economic impact. The implementation of innovative therapeutic alternatives for CVD is urgently required. Specialized proresolving lipid mediators (SPMs) are bioactive compounds derived from ω-3 and ω-6 fatty acids, integrated into four families: Lipoxins, Resolvins, Protectins, and Maresins. SPMs have generated interest in recent years due to their ability to promote the resolution of inflammation associated with the pathogeneses of numerous illnesses, particularly CVD. Several preclinical studies in animal models have evidenced their ability to decrease the progression of atherosclerosis, intimal hyperplasia, and reperfusion injury via diverse mechanisms. Large-scale clinical trials are required to determine the effects of SPMs in humans. This review integrates the currently available knowledge of the therapeutic impact of SPMs in CVD from preclinical and clinical studies, along with the implicated molecular pathways. In vitro results have been promising, and as such, SPMs could soon represent a new therapeutic alternative for CVD.
Extracellular Vesicles Secreted by Corneal Myofibroblasts Promote Corneal Epithelial Cell Migration
Authors: Yeung Vincent , Zhang Tancy C. , Yuan Ling , Parekh Mohit , Cortinas John A. , Delavogia Eleni , Hutcheon Audrey E. K. , Guo Xiaoqing , Ciolino Joseph B.
Published: March 2022

Corneal epithelial wound healing is a multifaceted process that encompasses cell proliferation, migration, and communication from the corneal stroma. Upon corneal injury, bidirectional crosstalk between the epithelium and stroma via extracellular vesicles (EVs) has been reported. However, the mechanisms by which the EVs from human corneal keratocytes (HCKs), fibroblasts (HCFs), and/or myofibroblasts (HCMs) exert their effects on the corneal epithelium remain unclear. In this study, HCK-, HCF-, and HCM-EVs were isolated and characterized, and human corneal epithelial (HCE) cell migration was assessed in a scratch assay following PKH26-labeled HCK-, HCF-, or HCM-EV treatment. HCE cells proliferative and apoptotic activity following EV treatment was assessed. HCF-/HCM-EVs were enriched for CD63, CD81, ITGAV, and THBS1 compared to HCK-EV. All EVs were negative for GM130 and showed minimal differences in biophysical properties. At the proteomic level, we showed HCM-EV with a log >two-fold change in CXCL6, CXCL12, MMP1, and MMP2 expression compared to HCK-/HCF-EVs; these proteins are associated with cellular movement pathways. Upon HCM-EV treatment, HCE cell migration, velocity, and proliferation were significantly increased compared to HCK-/HCF-EVs. This study concludes that the HCM-EV protein cargo influences HCE cell migration and proliferation, and understanding these elements may provide a novel therapeutic avenue for corneal wound healing.
Signal Sequence-Dependent Orientation of Signal Peptide Fragments to Exosomes
Authors: Ono Kenji , Niwa Mikio , Suzuki Hiromi , Kobayashi Nahoko Bailey , Yoshida Tetsuhiko , Sawada Makoto
Published: March 2022

Signal peptides (SPs) not only mediate targeting to the endoplasmic reticulum (ER) but also play important roles as biomarkers and substances with physiological activity in extracellular fluids including blood. SPs are thought to be degraded intracellularly, making it unclear how they are transported from the ER to the extracellular fluid. In a recent study, we showed that a C-terminal fragment of the SP of a type I membrane protein, amyloid precursor protein (APP), was secreted into the extracellular fluid via exosomes using transformed HEK293 cells expressing APP SP flanking a reporter protein. In the present study, we demonstrate that a N-terminal fragment of the SP from a type II membrane protein, human placental secreted alkaline phosphatase (SEAP), is contained in exosomes and secreted into the extracellular fluid using HEK-Blue hTLR3 cells, which express both a human toll-like receptor 3 gene and an inducible SEAP reporter gene. When HEK-Blue hTLR3 cells were stimulated with a TLR3 ligand, a N-terminal fragment of SEAP SP in exosomes was increased in parallel with SEAP secretion in a concentration-dependent manner. These results indicated that SP fragments are exosomal components. In addition, migrating SP fragments were determined by characteristics of the signal–anchor sequence of membrane proteins. Furthermore, we found that SP fragments could bind to calmodulin (CALM), which is a cytosolic protein and also a component of exosomes, suggesting its involvement in the transportation of SP fragments from the endoplasmic reticulum to exosomes.
Theoretical Characterization of the Step-by-Step Mechanism of Conversion of Leukotriene A4 to Leukotriene B4 Catalysed by the Enzyme Leukotriene A4 Hydrolase
Authors: Canyelles-Niño Miquel , González-Lafont Àngels , Lluch José M.
Published: March 2022

LTA4H is a bifunctional zinc metalloenzyme that converts leukotriene A4 (LTA4) into leukotriene B4 (LTB4), one of the most potent chemotactic agents involved in acute and chronic inflammatory diseases. In this reaction, LTA4H acts as an epoxide hydrolase with a unique and fascinating mechanism, which includes the stereoselective attachment of one water molecule to the carbon backbone of LTA4 several methylene units away from the epoxide moiety. By combining Molecular Dynamics simulations and Quantum Mechanics/Molecular Mechanics calculations, we obtained a very detailed molecular picture of the different consecutive steps of that mechanism. By means of a rather unusual 1,7-nucleophilic substitution through a clear SN1 mechanism, the epoxide opens and the triene moiety of the substrate twists in such a way that the bond C6-C7 adopts its cis (Z) configuration, thus exposing the R face of C12 to the addition of a water molecule hydrogen-bonded to ASP375. Thus, the two stereochemical features that are required for the bioactivity of LTB4 appear to be closely related. The noncovalent π-π stacking interactions between the triene moiety and two tyrosines (TYR267 and, especially, TYR378) that wrap the triene system along the whole reaction explain the preference for the cis configuration inside LTA4H.
An Upgrade on the Surveillance System of SARS-CoV-2: Deployment of New Methods for Genetic Inspection
Authors: Muñoz-Valle José Francisco , Venancio-Landeros Alberto Antony , Sánchez-Sánchez Rocío , Reyes-Díaz Karen , Galindo-Ornelas Byron , Hérnandez-Monjaraz Wendy Susana , García-Ríos Alejandra , García-Ortega Luis Fernando , Hernández-Bello Jorge , Peña-Rodríguez Marcela , Vega-Magaña Natali , Delaye Luis , Díaz-Sánchez Mauricio , García-González Octavio Patricio
Published: March 2022

SARS-CoV-2 variants surveillance is a worldwide task that has been approached with techniques such as Next Generation Sequencing (NGS); however, this technology is not widely available in developing countries because of the lack of equipment and limited funding in science. An option is to deploy a RT-qPCR screening test which aids in the analysis of a higher number of samples, in a shorter time and at a lower cost. In this study, variants present in samples positive for SARS-CoV-2 were identified with a RT-qPCR mutation screening kit and were later confirmed by NGS. A sample with an abnormal result was found with the screening test, suggesting the simultaneous presence of two viral populations with different mutations. The DRAGEN Lineage analysis identified the Delta variant, but there was no information about the other three mutations previously detected. When the sequenced data was deeply analyzed, there were reads with differential mutation patterns, that could be identified and classified in terms of relative abundance, whereas only the dominant population was reported by DRAGEN software. Since most of the software developed to analyze SARS-CoV-2 sequences was aimed at obtaining the consensus sequence quickly, the information about viral populations within a sample is scarce. Here, we present a faster and deeper SARS-CoV-2 surveillance method, from RT-qPCR screening to NGS analysis.
Synthesis of Multiple Bispecific Antibody Formats with Only One Single Enzyme Based on Enhanced Trypsiligase
Authors: Voigt Johanna , Meyer Christoph , Bordusa Frank
Published: March 2022

Bispecific antibodies (bsAbs) were first developed in the 1960s and are now emerging as a leading class of immunotherapies for cancer treatment with the potential to further improve clinical efficacy and safety. Many different formats of bsAbs have been established in the last few years, mainly generated genetically. Here we report on a novel, flexible, and fast chemo–enzymatic, as well as purely enzymatic strategies, for generating bispecific antibody fragments by covalent fusion of two functional antibody Fab fragments (Fabs). For the chemo–enzymatic approach, we first modified the single Fabs site-specifically with click anchors using an enhanced Trypsiligase variant (eTl) and afterward converted the modified Fabs into the final heterodimers via click chemistry. Regarding the latter, we used the strain-promoted alkyne-azide cycloaddition (SPAAC) and inverse electron-demand Diels–Alder reaction (IEDDA) click approaches well known for their fast reaction kinetics and fewer side reactions. For applications where the non-natural linkages or hydrophobic click chemistry products might interfere, we developed two purely enzymatic alternatives enabling C- to C- and C- to N-terminal coupling of the two Fabs via a native peptide bond. This simple system could be expanded into a modular system, eliminating the need for extensive genetic engineering. The bispecific Fab fragments (bsFabs) produced here to bind the growth factors ErbB2 and ErbB3 with similar KD values, such as the sole Fabs. Tested in breast cancer cell lines, we obtained biologically active bsFabs with improved properties compared to its single Fab counterparts.
Sovateltide Mediated Endothelin B Receptors Agonism and Curbing Neurological Disorders
Authors: Ranjan Amaresh K. , Gulati Anil
Published: March 2022

Neurological/neurovascular disorders constitute the leading cause of disability and the second leading cause of death globally. Major neurological/neurovascular disorders or diseases include cerebral stroke, Alzheimer’s disease, spinal cord injury, neonatal hypoxic-ischemic encephalopathy, and others. Their pathophysiology is considered highly complex and is the main obstacle in developing any drugs for these diseases. In this review, we have described the endothelin system, its involvement in neurovascular disorders, the importance of endothelin B receptors (ETBRs) as a novel potential drug target, and its agonism by IRL-1620 (INN—sovateltide), which we are developing as a drug candidate for treating the above-mentioned neurological disorders/diseases. In addition, we have highlighted the results of our preclinical and clinical studies related to these diseases. The phase I safety and tolerability study of sovateltide has shown it as a safe and tolerable compound at therapeutic dosages. Furthermore, preclinical and clinical phase II studies have demonstrated the efficacy of sovateltide in treating acute ischemic stroke. It is under development as a first-in-class drug. In addition, efficacy studies in Alzheimer’s disease (AD), acute spinal cord injury, and neonatal hypoxic-ischemic encephalopathy (HIE) are ongoing. Successful completion of these studies will validate that ETBRs signaling can be an important target in developing drugs to treat neurological/neurovascular diseases.