bluuebunny/crossref_metadata_2025 huggingface dataset:
Campaign Governance and Playfulness: Unraveling Chinese HPV Immunization Promotion Efforts on Douyin
Authors: Wu Jiaji , Yang Ronghui
Published: August 2024

(1) Background: Playful immunization promotion helps to approach persuasive efforts and raise vaccine acceptance. However, playful promotion in the field of HPV immunization has not been explored. (2) Methods: To address this gap, we analyzed data gleaned from 73 short videos posted by state media and from semi-structured interviews with 37 Chinese stakeholders using thematic analysis. (3) Results: The analysis revealed that state media promoted HPV immunization using celebrity endorsement, anthropomorphism techniques and entertainment performance strategies. Additionally, state media engaged in circle mobilization and livestreamed on Douyin to reach wider audiences. Although playful strategies increased the popularity of HPV vaccine promotion, insufficient multi-stakeholder partnerships and homogeneous message delivery decreased the efficiency of HPV immunization promotion campaigns. (4) Conclusions: The strengthening of multi-stakeholder partnerships and the optimization of the public service provision of HPV vaccination are expected. Our research will not only deepen the global audience’s understanding of Chinese immunization promotion campaigns, but also offer insights for implementing future global health promotion.
Strategies for Achieving High and Sustainable Plant Productivity in Saline Soil Conditions
Authors: Nurbekova Zhadyrassyn , Satkanov Mereke , Beisekova Moldir , Akbassova Alua , Ualiyeva Rimma , Cui Junfang , Chen Yangwu , Wang Zhaoqi , Zhangazin Sayan
Published: August 2024

The accumulation of salt in arable lands is a source of significant abiotic stress, contributing to a 10% decline in the world’s total arable lands and threatening food productivity and the sustainability of agriculture. About 76 million hectares of productive land are estimated to have been affected by human-induced salinization such as extreme salt deposits in soil, which are mainly caused by the actions of humans. For instance, continued irrigation and the frequent use of chemical fertilizers need to be understood. To ensure food availability, it is essential to improve upon traditional farming methods using current technologies to facilitate the reclamation of saline-affected arable lands to achieve high and sustainable food production. This review details current innovative strategies such as the modification of metabolic pathways, manipulation of antioxidant pathways, genetic engineering, RNA interference technology, engineered nanoparticles, arbuscular mycorrhizal fungi (AMF), organic amendments, and trace elements for improving saline marginal lands. These strategies were identified to have contributed to the improvement of plants salinity tolerance in diverse ways. For instance, the accumulation of plant metabolites such as amino acids, sugars, polyols, organic acids, saponins, anthocyanins, polyphenols, and tannins detoxify plants and play crucial roles in mitigating the detrimental effects of oxidative damage posed by salinity stress. Multiple plant miRNAs encoding the up- and down-regulation of single- and multi-ion transporters have been engineered in plant species to enhance salt tolerance. Nanomaterials and plant root system colonized by arbuscular mycorrhizal increase water uptake, photosynthetic efficiency, and biomass allocation in plants exposed to saline stress by excluding 65 percent of the Na+ uptake and enhancing K+ uptake by 84.21 percent. Organic amendments and trace elements reduced salinity concentrations by 22 percent and improved growth by up to 84 percent in maize subjected to salinity stress. This study also discusses how researchers can use these strategies to improve plants growth, development, and survival in saline soil conditions to enhance the productivity and sustainability of agriculture. The strategies discussed in this study have also proven to be promising approaches for developing salinity stress tolerance strategies for plants to increase agricultural productivity and sustainability.
Agricultural Economic Water Productivity Differences across Counties in the Colorado River Basin
Authors: Frisvold George B. , Atla Jyothsna
Published: August 2024

This study estimates the relative contribution of different factors to the wide variation in agricultural economic water productivity (EWP) across Colorado River Basin counties. It updates EWP measures for Basin counties using more detailed, localized data for the Colorado River mainstem. Using the Schwarz Bayesian Information Criterion for variable selection, regression analysis and productivity accounting methods identified factors contributing to EWP differences. The EWP was USD 1033 (USD 2023)/acre foot (af) for Lower Basin Counties on the U.S.–Mexico Border, USD 729 (USD 2023)/af for other Lower Basin Counties, and USD 168 (USD 2023)/af for Upper Basin Counties. Adoption rates for improved irrigation technologies showed little inter-county variation and so did not have a statistically significant impact on EWP. Counties with the lowest EWP consumed 25% of the Basin’s agricultural water (>2.3 million af) to generate 3% of the Basin’s crop revenue. Low populations/remoteness and more irrigated acreage per farm were negatively associated with EWP. Warmer winter temperatures and greater July humidity were positively associated with EWP. When controlling for other factors, being on the Border increased a county’s EWP by USD 570 (2023 USD)/af. Border Counties have greater access to labor from Mexico, enabling greater production of high-value, labor-intensive specialty crops.
Association of Physical and Emotional Parameters with Performance of Firefighters: A Systematic Review
Authors: Farinha Vinícius Montaguti , Borba Edilson Fernando de , Santos Poliana Piovezana dos , Ulbrich Anderson Zampier , Ribeiro Evaldo José Ferreira , Tartaruga Marcus Peikriszwili
Published: August 2024

Firefighting requires a high level of physical fitness and causes substantial psychological stress, engendering musculoskeletal, mental, and cardiac issues. Consequently, it is necessary to measure the preparation of the firefighters daily through the Firefighting Physical Ability Tests (FPATs). According to the literature, some variables are more important for performance in the FPAT. Therefore, we aimed to summarize evidence that relates physical and mental aspects to the FPAT performance. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) method, screening 1055 records from databases and selecting 15 that met inclusion criteria. No emotional and psychological variables were correlated with the FPAT. Most research shows significant correlations between the FPAT performance and the following: aerobic fitness, upper body endurance and strength, anaerobic capacity, body fat, and age. Lower body endurance and strength, as well as anaerobic power, had a low number of investigations and need to be further explored. Abdominal endurance showed weak correlations, while flexibility did not show any correlations in most studies, although these should be considered for injury prevention. We recommend that fitness programs and evaluations include a global analysis considering the evidence presented for methodological improvements.
Work-Related Musculoskeletal Problems: A Look at How Employers View Causes
Authors: Jensen Roger C.
Published: August 2024

Causation concepts for work-related musculoskeletal disorders vary among authors and academic disciplines. The major causation concepts are single-event and repetitive motion. The aim of this short communication is to share findings from a recent survey of United States employers conducted by the U.S. Bureau of Labor Statistics about work-related musculoskeletal cases, and, more specifically, about whether the employers regard the causes of their employees’ musculoskeletal problems as being from a single exposure or from multiple exposures. Recommendations are offered for using terminology consistent with employer understandings.
Exploring Mitochondrial Heterogeneity and Evolutionary Dynamics in Thelephora ganbajun through Population Genomics
Authors: Li Haixia , Liang Tong , Liu Yongju , Wang Pengfei , Wang Shaojuan , Zhao Min , Zhang Ying
Published: August 2024

Limited exploration in fungal mitochondrial genetics has uncovered diverse inheritance modes. The mitochondrial genomes are inherited uniparentally in the majority of sexual eukaryotes, our discovery of persistent mitochondrial heterogeneity within the natural population of the basidiomycete fungus Thelephora ganbajun represents a significant advance in understanding mitochondrial inheritance and evolution in eukaryotes. Here, we present a comprehensive analysis by sequencing and assembling the complete mitogenomes of 40 samples exhibiting diverse cox1 heterogeneity patterns from various geographical origins. Additionally, we identified heterogeneous variants in the nad5 gene, which, similar to cox1, displayed variability across multiple copies. Notably, our study reveals a distinct prevalence of introns and homing endonucleases in these heterogeneous genes. Furthermore, we detected potential instances of horizontal gene transfer involving homing endonucleases. Population genomic analyses underscore regional variations in mitochondrial genome composition among natural samples exhibiting heterogeneity. Thus, polymorphisms in heterogeneous genes, introns, and homing endonucleases significantly influence mitochondrial structure, structural variation, and evolutionary dynamics in this species. This study contributes valuable insights into mitochondrial genome architecture, population dynamics, and the evolutionary implications of mitochondrial heterogeneity in sexual eukaryotes.
Relation of T Cell Profile with Vitamin D Receptor and Vitamin D-Binding Protein Gene Polymorphisms in Atopy
Authors: Bastyte Daina , Tamasauskiene Laura , Stakaitiene Ieva , Briede Kamilija , Ugenskiene Rasa , Valiukeviciene Skaidra , Gradauskiene Brigita
Published: August 2024

Atopic diseases, including atopic dermatitis (AD) and allergic asthma (AA), are characterized by complex immune responses involving various T cells subsets and their cytokine profiles. It is assumed that single nucleotide polymorphisms (SNPs) in the Vitamin D receptor (VDR) gene and the Vitamin D-binding protein (GC) gene are related to the action of Vitamin D and, consequently, play a role in regulating the immune response. However, there is not enough data to unequivocally support the hypothesis about the relationship between T cells profile and VDR or GC SNPs. Two hundred sixty-six subjects (aged > 18 years) were involved in the study: 100 patients with mild or moderate AD, 85 patients with mild or moderate AA, and 81 healthy individuals. Blood cell counts were determined by standard methods. Flow cytometric analysis was used to evaluate CD4+ T-helper (Th) cell subtypes: Th2, Th1, Th17, and T regulatory (Treg) cells in peripheral blood. Measurements of cytokines, total immunoglobulin E (IgE), and Vitamin D levels in serum were evaluated by ELISA. Significantly higher levels of Th1, Th2, and Th17 cells, along with lower levels of Tregs, were found in patients with atopic diseases compared to healthy individuals. Additionally, higher serum levels of interleukin (IL) 5, IL-17A, and transforming growth factor-β1 (TGF-β1), as well as lower levels of IL-10, were observed in patients with atopic diseases than in control. The study established associations between VDR SNPs and immune profiles: the AA genotype of rs731236 was associated with increased Th2 and Th17 cells and a higher Th1/Th2 ratio; the GG genotype of rs731236 was linked to decreased serum IL-10 and TGF-β1 levels; and the TT genotype of rs11168293 was associated with increased IL-10 levels. Additionally, the GG genotype of GC gene SNP rs4588 was associated with reduced Th2 and Th17 lymphocytes, while the TT genotype of rs4588 was linked to decreased IL-10 levels. Furthermore, the CC genotype of rs7041 was associated with higher levels of Th2, Th17, IL-10, and IL-35, as well as reduced levels of TGF-β1, while the GG genotype of rs3733359 was associated with reduced IL-10 levels. In conclusion, our study demonstrates that the Vitamin D receptor gene single nucleotide polymorphisms rs731236 and rs11168293, along with polymorphisms in the Vitamin D-binding protein gene (rs4588, rs7041, rs3733359), are significantly associated with variations in T cell profiles in atopy. These variations may play a crucial role in promoting inflammation and provide insight into the genetic factors contributing to the pathogenesis of atopy.
Molecular Signatures of CB-6644 Inhibition of the RUVBL1/2 Complex in Multiple Myeloma
Authors: Yi Weijun , Dziadowicz Sebastian A. , Mangano Rachel S. , Wang Lei , McBee Joseph , Frisch Steven M. , Hazlehurst Lori A. , Adjeroh Donald A. , Hu Gangqing
Published: August 2024

Multiple myeloma is the second most hematological cancer. RUVBL1 and RUVBL2 form a subcomplex of many chromatin remodeling complexes implicated in cancer progression. As an inhibitor specific to the RUVBL1/2 complex, CB-6644 exhibits remarkable anti-tumor activity in xenograft models of Burkitt’s lymphoma and multiple myeloma (MM). In this work, we defined transcriptional signatures corresponding to CB-6644 treatment in MM cells and determined underlying epigenetic changes in terms of chromatin accessibility. CB-6644 upregulated biological processes related to interferon response and downregulated those linked to cell proliferation in MM cells. Transcriptional regulator inference identified E2Fs as regulators for downregulated genes and MED1 and MYC as regulators for upregulated genes. CB-6644-induced changes in chromatin accessibility occurred mostly in non-promoter regions. Footprinting analysis identified transcription factors implied in modulating chromatin accessibility in response to CB-6644 treatment, including ATF4/CEBP and IRF4. Lastly, integrative analysis of transcription responses to various chemical compounds of the molecular signature genes from public gene expression data identified CB-5083, a p97 inhibitor, as a synergistic candidate with CB-6644 in MM cells, but experimental validation refuted this hypothesis.
Circulating Bacterial DNA in Colorectal Cancer Patients: The Potential Role of Fusobacterium nucleatum
Authors: Koliarakis Ioannis , Lagkouvardos Ilias , Vogiatzoglou Konstantinos , Tsamandouras Ioannis , Intze Evangelia , Messaritakis Ippokratis , Souglakos John , Tsiaoussis John
Published: August 2024

Intestinal dysbiosis is a major contributor to colorectal cancer (CRC) development, leading to bacterial translocation into the bloodstream. This study aimed to evaluate the presence of circulated bacterial DNA (cbDNA) in CRC patients (n = 75) and healthy individuals (n = 25). DNA extracted from peripheral blood was analyzed using PCR, with specific primers targeting 16S rRNA, Escherichia coli (E. coli), and Fusobacterium nucleatum (F. nucleatum). High 16S rRNA and E. coli detections were observed in all patients and controls. Only the detection of F. nucleatum was significantly higher in metastatic non-excised CRC, compared to controls (p < 0.001), non-metastatic excised CRC (p = 0.023), and metastatic excised CRC (p = 0.023). This effect was mainly attributed to the presence of the primary tumor (p = 0.006) but not the presence of distant metastases (p = 0.217). The association of cbDNA with other clinical parameters or co-morbidities was also evaluated, revealing a higher detection of E. coli in CRC patients with diabetes (p = 0.004). These results highlighted the importance of bacterial translocation in CRC patients and the potential role of F. nucleatum as an intratumoral oncomicrobe in CRC.
MicroRNAs Participate in Morphological Acclimation of Sugar Beet Roots to Nitrogen Deficiency
Authors: Liu Xinyu , Lu Zhenqiang , Yao Qi , Xu Lingqing , Fu Jingjing , Yin Xilong , Bai Qing , Liu Dali , Xing Wang
Published: August 2024

Nitrogen (N) is essential for sugar beet (Beta vulgaris L.), a highly N-demanding sugar crop. This study investigated the morphological, subcellular, and microRNA-regulated responses of sugar beet roots to low N (LN) stress (0.5 mmol/L N) to better understand the N perception, uptake, and utilization in this species. The results showed that LN led to decreased dry weight of roots, N accumulation, and N dry matter production efficiency, along with damage to cell walls and membranes and a reduction in organelle numbers (particularly mitochondria). Meanwhile, there was an increase in root length (7.2%) and branch numbers (29.2%) and a decrease in root surface area (6.14%) and root volume (6.23%) in sugar beet after 7 d of LN exposure compared to the control (5 mmol/L N). Transcriptomics analysis was confirmed by qRT-PCR for 6 randomly selected microRNAs, and we identified 22 differentially expressed microRNAs (DEMs) in beet root under LN treatment. They were primarily enriched in functions related to binding (1125), ion binding (641), intracellular (437) and intracellular parts (428), and organelles (350) and associated with starch and sucrose metabolism, tyrosine metabolism, pyrimidine metabolism, amino sugar and nucleotide sugar metabolism, and isoquinoline alkaloid biosynthesis, as indicated by the GO and KEGG analyses. Among them, the upregulated miR156a, with conserved sequences, was identified as a key DEM that potentially targets and regulates squamosa promoter-binding-like proteins (SPLs, 104889216 and 104897537) through the microRNA-mRNA network. Overexpression of miR156a (MIR) promoted root growth in transgenic Arabidopsis, increasing the length, surface area, and volume. In contrast, silencing miR156a (STTM) had the opposite effect. Notably, the fresh root weight decreased by 45.6% in STTM lines, while it increased by 27.4% in MIR lines, compared to the wild type (WT). It can be inferred that microRNAs, especially miR156, play crucial roles in sugar beet root’s development and acclimation to LN conditions. They likely facilitate active responses to N deficiency through network regulation, enabling beet roots to take up nutrients from the environment and sustain their vital life processes.
Discovery of Novel Amino Acids (Analogues)-Substituted Thiophene[3,2-d]pyrimidine Derivatives as Potent HIV-1 Non-Nucleoside Reverse Transcriptase Inhibitors: Design, Synthesis, and Biological Evaluation
Authors: Zhuo Zongji , Wang Zhao , Jing Lanlan , Zhang Tao , Ge Anchao , Zhou Zhenzhen , Liu Ying , Li Xin , De Clercq Erik , Pannecouque Christophe , Zhan Peng , Liu Xinyong , Kang Dongwei
Published: August 2024

Inspired by our previous work on the modification of diarylpyrimidine-typed non-nucleoside reverse transcriptase inhibitors (NNRTIs) and the reported crystallographic studies, a series of novel amino acids (analogues)-substituted thiophene[3,2-d]pyrimidine derivatives were designed and synthesized by targeting the solvent-exposed region of the NNRTI-binding pocket. The biological evaluation results showed that compound 5k was the most active inhibitor, exhibiting moderate-to-excellent potency against HIV-1 wild-type (WT) and a panel of NNRTI-resistant strains, with EC50 values ranging from 0.042 μM to 7.530 μM. Of special note, 5k exhibited the most potent activity against single-mutant strains (K103N and E138K), with EC50 values of 0.031 μM and 0.094 μM, being about 4.3-fold superior to EFV (EC50 = 0.132 μM) and 1.9-fold superior to NVP (EC50 = 0.181 μM), respectively. In addition, 5k demonstrated lower cytotoxicity (CC50 = 27.9 μM) and higher selectivity index values. The HIV-1 reverse transcriptase (RT) inhibition assay was further performed to confirm their binding target. Moreover, preliminary structure–activity relationships (SARs) and molecular docking studies were also discussed in order to provide valuable insights for further structural optimizations. In summary, 5k turned out to be a promising NNRTI lead compound for further investigations of treatments for HIV-1 infections.
Effects of Fasting on THP1 Macrophage Metabolism and Inflammatory Profile
Authors: Rius-Bonet Julia , Macip Salvador , Massip-Salcedo Marta , Closa Daniel
Published: August 2024

Fasting can affect the body’s inflammatory response, and this has been linked to potential health benefits, including improvements for people with rheumatic diseases. In this work, we evaluated, in vitro, how changes in nutrient availability alter the inflammatory response of macrophages. Macrophage-differentiated THP1 cells were cultured, deprived of FCS or subjected to cycles of FCS deprivation and restoration to mimic intermittent fasting. Changes in the macrophage phenotype, the cells’ response to inflammatory stimuli and the level of mitochondrial alteration were assessed. The results indicate that while periods of serum starvation are associated with a decrease in IL1β and TNFα expression, consistent with an anti-inflammatory response, intermittent serum starvation cycles promote a pro-inflammatory phenotype. Rapid changes in reducing capacity and mitochondrial response were also observed. Of note, while some changes, such as the production of oxygen free radicals, were reversed with refeeding, others, such as a decrease in reducing capacity, were maintained and even increased. This study shows that different fasting protocols can have diverging effects and highlights that time-limited nutrient changes can significantly affect macrophage functions in cell cultures. These findings help elucidate some of the mechanisms by which specific fasting dietary interventions could help control inflammatory diseases.
Wilson’s Disease—Crossroads of Genetics, Inflammation and Immunity/Autoimmunity: Clinical and Molecular Issues
Authors: Gromadzka Grażyna , Czerwińska Julia , Krzemińska Elżbieta , Przybyłkowski Adam , Litwin Tomasz
Published: August 2024

Wilson’s disease (WD) is a rare, autosomal recessive disorder of copper metabolism caused by pathogenic mutations in the ATP7B gene. Cellular copper overload is associated with impaired iron metabolism. Oxidative stress, cuproptosis, and ferroptosis are involved in cell death in WD. The clinical picture of WD is variable. Hepatic/neuropsychiatric/other symptoms may manifest in childhood/adulthood and even old age. It has been shown that phenotypic variability may be determined by the type of ATP7B genetic variants as well as the influence of various genetic/epigenetic, environmental, and lifestyle modifiers. In 1976, immunological abnormalities were first described in patients with WD. These included an increase in IgG and IgM levels and a decrease in the percentage of T lymphocytes, as well as a weakening of their bactericidal effect. Over the following years, it was shown that there is a bidirectional relationship between copper and inflammation. Changes in serum cytokine concentrations and the relationship between cytokine gene variants and the clinical course of the disease have been described in WD patients, as well as in animal models of this disease. Data have also been published on the occurrence of antinuclear antibodies (ANAs), antineutrophil cytoplasmic antibodies (ANCAs), anti-muscle-specific tyrosine kinase antibodies, and anti-acetylcholine receptor antibodies, as well as various autoimmune diseases, including systemic lupus erythematosus (SLE), myasthenic syndrome, ulcerative colitis, multiple sclerosis (MS), polyarthritis, and psoriasis after treatment with d-penicillamine (DPA). The occurrence of autoantibodies was also described, the presence of which was not related to the type of treatment or the form of the disease (hepatic vs. neuropsychiatric). The mechanisms responsible for the occurrence of autoantibodies in patients with WD are not known. It has also not been clarified whether they have clinical significance. In some patients, WD was differentiated or coexisted with an autoimmune disease, including autoimmune hepatitis or multiple sclerosis. Various molecular mechanisms may be responsible for immunological abnormalities and/or the inflammatory processes in WD. Their better understanding may be important for explaining the reasons for the diversity of symptoms and the varied course and response to therapy, as well as for the development of new treatment regimens for WD.
A Bioinformatics-Based Study on Methylation Alterations of the FBLN1 Gene in Hippocampal Tissue of Alzheimer’s Disease Model DKO and DTG Mice
Authors: Tang Rui , Jian Yue , Hu Zhimin , Li Li , Wang Haitao , Miao Pengyu , Yang Zhihui , Tang Mingxi
Published: August 2024

Alzheimer’s disease (AD) is characterized by progressive cognitive decline and late-stage neurobehavioral issues marked by amyloid-beta plaques and Tau protein tangles. This study aims to investigate Fibulin-1(FBLN1) gene expression in the hippocampal tissue of Presenilin-1/Presenilin-2 conditional double-knockout (DKO) and double-transgenic (DTG) mice, using single-cell sequencing and experimental methods to verify abnormal methylation status and correlation with AD. Genomic DNA from DKO and DTG mice was used for genotyping. Reduced Representation Bisulfite Sequencing (RRBS) identified 10 genes with abnormal methylation changes, with protein–protein interaction (PPI) analysis highlighting five core genes, including FBLN1. Single-cell sequencing, RT-PCR, and Western blotting (WB) were used to analyze FBLN1 mRNA and protein levels in the hippocampal tissues of early-stage and mid-stage AD DKO, DTG, and CBAC57 mice. RRBS identified 10 genes with abnormal methylation, with PPI highlighting five core genes. Single-cell sequencing showed significant FBLN1 expression in AD groups. RT-PCR and WB indicated elevated FBLN1 mRNA and protein levels in mid-stage AD DKO and DTG mice compared to CBAC57 mice, with no differences in early-stage AD DKO and CBAC57 mice. RRBS revealed hypomethylation of the FBLN1 gene in mid-stage AD DKO mice. Elevated FBLN1 expression in AD models suggests an age-dependent neurodegenerative mechanism independent of amyloid-beta deposition. This study enhances our understanding of AD’s epigenetic mechanisms, which will aid targeted diagnosis, treatment, and prognosis.
Brassicaceae Mustards: Phytochemical Constituents, Pharmacological Effects, and Mechanisms of Action against Human Disease
Authors: Rahman Mahmudur , Khatun Amina , Liu Lei , Barkla Bronwyn J.
Published: August 2024

The Brassicaceae genus consists of many economically important mustards of value for food and medicinal purposes, namely Asian mustard (Brassica juncea), ball mustard (Neslia paniculata), black mustard (B. nigra), garlic mustard (Alliaria petiolata), hedge mustard (Sisymbrium officinale), Asian hedge mustard (S. orientale), oilseed rape (B. napus), rapeseed (B. rapa), treacle mustard (Erysimum repandum), smooth mustard (S. erysimoides), white ball mustard (Calepina irregularis), white mustard (Sinapis alba), and Canola. Some of these are commercially cultivated as oilseeds to meet the global demand for a healthy plant-derived oil, high in polyunsaturated fats, i.e., B. napus and B. juncea. Other species are foraged from the wild where they grow on roadsides and as a weed of arable land, i.e., E. repandum and S. erysimoides, and harvested for medicinal uses. These plants contain a diverse range of bioactive natural products including sulfur-containing glucosinolates and other potentially valuable compounds, namely omega-3-fatty acids, terpenoids, phenylpropanoids, flavonoids, tannins, S-methyl cysteine sulfoxide, and trace-elements. Various parts of these plants and many of the molecules that are produced throughout the plant have been used in traditional medicines and more recently in the mainstream pharmaceutical and food industries. This study relates the uses of mustards in traditional medicines with their bioactive molecules and possible mechanisms of action and provides an overview of the current knowledge of Brassicaceae oilseeds and mustards, their phytochemicals, and their biological activities.
The Complex World of Kynurenic Acid: Reflections on Biological Issues and Therapeutic Strategy
Authors: Stone Trevor W. , Darlington L. Gail , Badawy Abdulla A.-B. , Williams Richard O.
Published: August 2024

It has been unequivocally established that kynurenic acid has a number of actions in a variety of cells and tissues, raising, in principle, the possibility of targeting its generation, metabolism or sites of action to manipulate those effects to a beneficial therapeutic end. However, many basic aspects of the biology of kynurenic acid remain unclear, potentially leading to some confusion and misinterpretations of data. They include questions of the source, generation, targets, enzyme expression, endogenous concentrations and sites of action. This essay is intended to raise and discuss many of these aspects as a source of reference for more balanced discussion. Those issues are followed by examples of situations in which modulating and correcting kynurenic acid production or activity could bring significant therapeutic benefit, including neurological and psychiatric conditions, inflammatory diseases and cell protection. More information is required to obtain a clear overall view of the pharmacological environment relevant to kynurenic acid, especially with respect to the active concentrations of kynurenine metabolites in vivo and changed levels in disease. The data and ideas presented here should permit a greater confidence in appreciating the sites of action and interaction of kynurenic acid under different local conditions and pathologies, enhancing our understanding of kynurenic acid itself and the many clinical conditions in which manipulating its pharmacology could be of clinical value.
Innovations in Early Lung Cancer Detection: Tracing the Evolution and Advancements in Screening
Authors: Cotton Lindsey B. , Bach Peter B. , Cisar Chris , Schonewolf Caitlin A. , Tennefoss Demetria , Vachani Anil , Carter-Bawa Lisa , Zaidi Ali H.
Published: August 2024

Lung cancer mortality rates, particularly non-small cell lung cancer (NSCLC), continue to present a significant global health challenge, and the adoption of lung cancer screening remains limited, often influenced by inequities in access to healthcare. Despite clinical evidence demonstrating the efficacy of annual screening with low-dose computed tomography (LDCT) and recommendations from medical organizations including the U.S. Preventive Services Task Force (USPSTF), the national lung cancer screening uptake remains around 5% among eligible individuals. Advancements in the clinical management of NSCLC have recently become more personalized with the implementation of blood-based biomarker testing. Extensive research into tumor-derived cell-free DNA (cfDNA) through fragmentation offers a novel method for improving early lung cancer detection. This review assesses the screening landscape, explores obstacles to lung cancer screening, and discusses how a plasma whole genome fragmentome test (pWGFrag-Lung) can improve lung cancer screening participation and adherence.
The Usefulness of the CHA2DS2-VASc Score to Predict Outcomes in Patients with Infective Endocarditis
Authors: Itelman Edward , Sharony Ram , Hamdan Ashraf , Atamna Alaa , Shaked Hila , Rubchevsky Victor , Barak Yaron D. , Bernstine Hanna , Shapira Yaron , Vaturi Mordehay , Epstein Hadass Ofek , Kornowski Ran , Orvin Katia
Published: August 2024

Introduction: Despite diagnostic and therapeutic advances, infective endocarditis (IE) is still associated with high mortality rates. Currently, there are no good prognostic tools for the risk assessment of patients with IE. The CHA2DS2-VASc score, used to estimate the risk of ischemic stroke in patients with non-valvular atrial fibrillation (AF), has been shown to be a powerful predictor of stroke and death in patients without known AF associated with other cardiovascular conditions. Objective: We aimed to evaluate the usefulness of the CHA2DS2-VASc score as a prognostic tool in a population of patients with IE. Methods: The Rabin Medical Center Endocarditis Team (RMCET) registry is a retrospective cohort of all patients evaluated at our center due to acute or sub-acute bacterial endocarditis. The CHA2DS2-VASc score was extracted for all patients. All-cause mortality was depicted for all patients. Results: The cohort included 330 patients with a mean age of 65.2 ± 14.7 years (70% men). During a median follow-up of 24 months [IQR 4.7–48.6], 121 (36.7%) patients died. The median CHA2DS2-VASc score was 3, and any score above 2 was associated with increased overall mortality (50.8% vs. 19.9%, p < 0.001). A multivariate model incorporating important confounders not included in the CHA2DS2-VASc model showed consistent results with a risk increase of 121% for the higher CHA2DS2-VASc score groups (HR 2.21 [CI 1.12–4.39], p = 0.023). Conclusions: IE currently has no good risk stratification models for clinical practice. The CHA2DS2-VASc score might serve as a simple and available tool to stratify risk among patients with IE.
A Comparative Study of Airbag Covers for Automotive Safety Using Coconut Shell Fiber/PP Composite Materials
Authors: Li Jinsong , Zhou You , Chen Jiatao , Hu Hongtao , Sun Mingze
Published: August 2024

In this study, we compared the physical properties of coconut fiber/polypropylene (PP) composite materials with coconut fiber as a reinforcing agent, produced through a hybrid injection molding process and a layered hot-pressing process. Through comparative experiments, the mechanical properties of both the hybrid injection-molded and layered hot-pressed materials were validated. The results indicated that, when using a coconut fiber content of 5%, the layered hot-pressed composite material exhibited optimal comprehensive performance. Specifically, its tensile strength reached 25.12 MPa, showing a 37.6% increase over that of pure PP materials of the same brand and batch. Its tensile modulus was 1.17 GPa, representing an 11.4% decrease. Additionally, its bending strength was 35.94 MPa, marking a 49.8% increase, and its bending modulus was 2.69 GPa, which is nearly double that of pure PP materials. Furthermore, through Creo modeling and an ANSYS simulation analysis, it was verified that this material could be applied to airbag covers in the field of automotive safety. This study confirmed that layered hot-pressed coconut fiber/PP composite materials exhibit superior mechanical properties to traditional materials and injection-molded composite materials, making them more suitable for airbag covers.
External Load Evaluation in Elite Futsal: Influence of Match Results and Game Location with IMU Technology
Authors: Gadea-Uribarri Héctor , Lago-Fuentes Carlos , Bores-Arce Ainhoa , Villavicencio Álvarez Víctor Emilio , López-García Sergio , Calero-Morales Santiago , Mainer-Pardos Elena
Published: August 2024

The purpose of this study was to assess the external load demands in futsal, considering both home and away matches and their outcomes, in order to plan microcycles throughout the season based on the external load of each match. The external load of 10 players from a First Division team in the Spanish Futsal League was recorded throughout 15 official matches in the first half of the league championship. The players’ external load was monitored using OLIVER devices. To analyse the influence of the match outcome and location on the external load, a univariate general linear model (GLM) analysis was conducted with Bonferroni post hoc. There are no differences between the variables neither comparing results nor location factors, except for accelerations of 2 to 3 m/s2 (m) per minute and the number of accelerations of 2 to 3 m/s2 per minute, reporting higher value winnings at home than away (p < 0.05). The location and results are not factors that influence on external load in futsal matches, except the number and distance performed in accelerations and distance covered at a low to medium speed. These findings are important for planning microcycles and providing the appropriate dosage to each player to achieve optimal performance in matches.
Containment-Based Distributed Secondary Control for AC Shipboard Microgrids under General Noise
Authors: Wang Liangbin , Teng Fei , Xu Qi
Published: August 2024

This paper investigates the secondary control problem of shipboard microgrids (SMGs) with a high percentage of new energy sources under general noise. Firstly, a polymorphic SMG model is constructed, which enables the software-defined functionality of the control strategy and allows heterogeneous distributed generators (DGs) in AC SMGs to exchange packets of different types. Secondly, due to the presence of highly dynamic and high-power loads in the SMGs, a containment-based distributed secondary control strategy is proposed to improve the flexibility of the DG voltage regulation. Then, considering the complexity and diversity of disturbances during ship navigation, general noise is introduced instead of white noise to describe various disturbances. Furthermore, based on the random differential equations (RDEs), the NOS stability of the proposed strategy is proved using Lyapunov theory, which proves the effectiveness of the containment-based distributed secondary control strategy under general noise. And, the containment error is obtained to prove that the voltage and frequency of the system converge to the convex hull spanned by the virtual leaders, ensuring the high quality of the power supply. Finally, the validity of the proposed containment-based strategy is verified by an AC SMG model with four DGs in three cases.
Plasma Parathormone Levels during Citrate Anticoagulated Continuous Venovenous Hemofiltration in ICU Patients
Authors: Elzo Kraemer Carlos V. , Appelman-Dijkstra Natasha M. , Ballieux Bart E. P. B. , du Fossé Nadia A. , van Westerloo David J. , de Jonge Evert
Published: August 2024

Continuous venovenous hemofiltation (CVVH) with citrate anticoagulation has been shown to be associated with substantial losses of calcium and negative calcium balance in ICU patients, which may lead to excessive bone loss and osteoporosis. The aim of this study is to investigate whether plasma parathormone monitoring can identify patients with negative calcium balance during CVVH. This is a retrospective single-center study of all adult ICU patients treated with citrate CVVH from 2021 to 2023. PTH was measured routinely once per week. Calcium excretion in ultrafiltrate fluid and CVVH calcium balance were measured daily. In total, 274 PTH measurements were performed in 111 patients. In 61 measurements (22%), PTH was higher than the upper limit of normal (>8 pmol/L). If PTH was higher than normal, plasma ionized calcium was less than 1.16 mmol/L in 77% of cases and hypercalcemia was never present. In a subgroup of patients treated with CVVH for at least 36 h in the preceding 72 h, PTH values were similar for quartiles by cumulative calcium balance. Increased plasma concentrations of PTH are frequently found in ICU patients treated with citrate CVVH, but no association was found between PTH and the CVVH calcium balance over the last 72 h.
Land-Use Conflict Dynamics, Patterns, and Drivers under Rapid Urbanization
Authors: Wang Guojian , Wang Jianguo , Wang Lingzhi , Zhang Yi , Zhang Wenxuan
Published: August 2024

Conflict over land use is an issue that all countries are experiencing in the accelerated process of urbanization and industrialization. Research on the identification and characterization of land-use conflicts is an important basis for promoting the sustainable development of regional land use. Taking Hebei Province under the background of Beijing–Tianjin–Hebei integration as the research object, this article combines the SCCI model and the LUF model to study the land-use flush in the process of rapid urbanization from the dimensions of land-use landscape conflict and land-use function conflict. The results of this study point out that land-use conflicts in the region have gone through a developmental course of intensification of heavy conflicts, slowing down, and then smoothing out. The exacerbation of land-use conflicts is synchronized with the time pattern of construction and development in the accelerated industrialization and urbanization of Hebei, while the activities of arable land occupation and compensation balance and land ecological management produce lagging land-use conflicts. The spatial pattern is characterized by dispersed and random conflicts in the plains, concentrated conflicts in the mountain stream zones, and stable conflicts in the ecological zones within the mountains in the mountainous areas. The role of externalities and internalities from within the region and in the coordinated development of the region has led to the coexistence of developmental and governance land-use conflicts in Hebei Province, and the geographic environment has a constraining effect on the spatial differentiation of these conflicts. Along with the strong implementation of China’s eco-governance and use-control systems, developmental land-use conflicts from the region will be effectively curbed, but the risk of overlapping developmental conflicts and lagging governance conflicts from coordinated regional development is a key focus for conflict prevention in the future.
TMEM9B Regulates Endosomal ClC-3 and ClC-4 Transporters
Authors: Festa Margherita , Coppola Maria Antonietta , Angeli Elena , Tettey-Matey Abraham , Giusto Alice , Mazza Irene , Gatta Elena , Barbieri Raffaella , Picollo Alessandra , Gavazzo Paola , Pusch Michael , Picco Cristiana , Sbrana Francesca
Published: August 2024

The nine-member CLC gene family of Cl− chloride-transporting membrane proteins is divided into plasma membrane-localized Cl− channels and endo-/lysosomal Cl−/H+ antiporters. Accessory proteins have been identified for ClC-K and ClC-2 channels and for the lysosomal ClC-7, but not the other CLCs. Here, we identified TMEM9 Domain Family Member B (TMEM9B), a single-span type I transmembrane protein of unknown function, to strongly interact with the neuronal endosomal ClC-3 and ClC-4 transporters. Co-expression of TMEM9B with ClC-3 or ClC-4 dramatically reduced transporter activity in Xenopus oocytes and transfected HEK cells. For ClC-3, TMEM9B also induced a slow component in the kinetics of the activation time course, suggesting direct interaction. Currents mediated by ClC-7 were hardly affected by TMEM9B, and ClC-1 currents were only slightly reduced, demonstrating specific interaction with ClC-3 and ClC-4. We obtained strong evidence for direct interaction by detecting significant Förster Resonance Energy Transfer (FRET), exploiting fluorescence lifetime microscopy-based (FLIM-FRET) techniques between TMEM9B and ClC-3 and ClC-4, but hardly any FRET with ClC-1 or ClC-7. The discovery of TMEM9B as a novel interaction partner of ClC-3 and ClC-4 might have important implications for the physiological role of these transporters in neuronal endosomal homeostasis and for a better understanding of the pathological mechanisms in CLCN3- and CLCN4-related pathological conditions.
Impacts of Interleukin-10 Promoter Genotypes on Prostate Cancer
Authors: Chin Yu-Ting , Tsai Chung-Lin , Ma Hung-Huan , Cheng Da-Chuan , Tsai Chia-Wen , Wang Yun-Chi , Shih Hou-Yu , Chang Shu-Yu , Gu Jian , Chang Wen-Shin , Bau Da-Tian
Published: August 2024

Prostate cancer (PCa) is a multifactorial disease influenced by genetic, environmental, and immunological factors. Genetic polymorphisms in the interleukin-10 (IL-10) gene have been implicated in PCa susceptibility, development, and progression. This study aims to assess the contributions of three IL-10 promoter single nucleotide polymorphisms (SNPs), A-1082G (rs1800896), T-819C (rs3021097), and A-592C (rs1800872), to the risk of PCa in Taiwan. The three IL-10 genotypes were determined using PCR-RFLP methodology and were evaluated for their contributions to PCa risk among 218 PCa patients and 436 non-PCa controls. None of the three IL-10 SNPs were significantly associated with the risks of PCa (p all > 0.05) in the overall analyses. However, the GG at rs1800896 combined with smoking behavior was found to significantly increase the risk of PCa by 3.90-fold (95% confidence interval [95% CI] = 1.28–11.89, p = 0.0231). In addition, the rs1800896 AG and GGs were found to be correlated with the late stages of PCa (odds ratio [OR] = 1.90 and 6.42, 95% CI = 1.05–3.45 and 2.30–17.89, p = 0.0452 and 0.0003, respectively). The IL-10 promoter SNP, A-1082G (rs1800896), might be a risk factor for PCa development among smokers and those at late stages of the disease. These findings should be validated in larger and more diverse populations.
The Effective Separation of Gallium, Vanadium, and Aluminum from a Simulated Bayer Solution by Resin Exchange
Authors: Qin Zhifeng , Jin Xi , Yang Zhen , Xin Yuntao , Liu Weizao
Published: August 2024

The effective recovery of gallium from wastewater discharge in the Bayer process is promising for the long-term development of gallium resources. The adsorption and desorption behavior of gallium (Ga), vanadium (V), and aluminum (Al) ions on a strong acidic styrene cation exchange resin (JK resin) from a simulated Bayer solution was systematically investigated by static experiments. The results showed that the optimum conditions for separating Ga from V and Al were at low temperatures and short contact times, with 78.30%, 15.16%, and 6.63% of the adsorption efficiency at 25 °C and 60 min, respectively, for Ga, V, and Al. The adsorption kinetics of Ga3+ conformed to the pseudo-second order model, and the static saturation adsorption capacity was 18.25 mg/g. The Langmuir model fitted the adsorption isotherm of gallium well, and the maximum adsorption capacity was 1.11 mg/g at 25 °C. FT-IR spectroscopy and XPS showed that the mechanism of the Ga3+ adsorption was only related to the interaction of the oxygen atoms of the amide oxime group (C=NOH). The separation of Ga, V, and Al can be achieved by desorbing 98% of Al with low concentrations of ammonia and 90% of Ga with low concentrations of hydrochloric acid. The results indicate that JK resin is an efficient adsorbent for separating gallium and vanadium in alkaline solutions.
Laser-Assisted Preparation of TiO2/Carbon/Ag Nanocomposite for Degradation of Organic Pollutants
Authors: Almasi Nezhad Shahin , Jaleh Babak , Darabi Elham , Dorranian Davoud
Published: August 2024

The ever-increasing expansion of chemical industries produces a variety of common pollutants, including colors, which become a global and environmental problem. Using a nanocatalyst is one of the effective ways to reduce these organic contaminants. With this in mind, a straightforward and effective method for the production of a novel nanocatalyst based on lignin-derived carbon, titanium dioxide nanoparticles, and Ag particles (TiO2/C/Ag) is described. The preparation of carbon and Ag particles (in sub-micro and nano size) was carried out by laser ablation in air. The nanocomposite was synthesized using a facile magnetic stirrer of TiO2, C, and Ag. According to characterization methods, a carbon nanostructure was successfully synthesized through the laser irradiation of lignin. According to scanning electron microscope images, spherical Ag particles were agglomerated over the nanocomposite. The catalytic activities of the TiO2/C/Ag nanocomposite were tested for the decolorization of methylene blue (MB) and Congo red (CR), employing NaBH4 in a water-based solution at 25 °C. After adding fresh NaBH4 to the mixture of nanocomposite and dyes, both UV absorption peaks of MB and CR completely disappeared after 10 s and 4 min, respectively. The catalytic activity of the TiO2/C/Ag nanocomposite was also examined for the reduction of 4-nitrophenol (4-NP) using a NaBH4 reducing agent, suggesting the complete reduction of 4-NP to 4-aminophenol (4-AP) after 2.30 min. This shows excellent catalytic behavior of the prepared nanocomposite in the reduction of organic pollutants.