bluuebunny/crossref_metadata_2025 huggingface dataset:
Iowa Implementation for Sustainability Framework
Authors: Cullen Laura , Hanrahan Kirsten , Edmonds Stephanie W. , Reisinger Heather Schacht , Wagner Michele
Published: January 2022

Background

An application-oriented implementation framework designed for clinicians and based on the Diffusion of Innovations theory included 81 implementation strategies with suggested timing for use within four implementation phases. The purpose of this research was to evaluate and strengthen the framework for clinician use and propose its usefulness in implementation research.

Methods

A multi-step, iterative approach guided framework revisions. Individuals requesting the use of the framework over the previous 7 years were sent an electronic questionnaire. Evaluation captured framework usability, generalizability, accuracy, and implementation phases for each strategy. Next, nurse leaders who use the framework pile sorted strategies for cultural domain analysis. Last, a panel of five EBP/implementation experts used these data and built consensus to strengthen the framework.

Results

Participants (n = 127/1578; 8% response) were predominately nurses (94%), highly educated (94% Master’s or higher), and from across healthcare (52% hospital/system, 31% academia, and 7% community) in the USA (84%). Most (96%) reported at least some experience using the framework and 88% would use the framework again. A 4-point scale (1 = not/disagree to 4 = very/agree) was used. The framework was deemed useful (92%, rating 3–4), easy to use (72%), intuitive (67%), generalizable (100%), flexible and adaptive (100%), with accurate phases (96%), and accurate targets (100%). Participants (n = 51) identified implementation strategy timing within four phases (Cochran’s Q); 54 of 81 strategies (66.7%, p < 0.05) were significantly linked to a specific phase; of these, 30 (55.6%) matched the original framework. Next, nurse leaders (n = 23) completed a pile sorting activity. Anthropac software was used to analyze the data and visualize it as a domain map and hierarchical clusters with 10 domains. Lastly, experts used these data and implementation science to refine and specify each of the 75 strategies, identifying phase, domain, actors, and function. Strategy usability, timing, and groupings were used to refine the framework.

Conclusion

The Iowa Implementation for Sustainability Framework offers a typology to guide implementation for evidence-based healthcare. This study specifies 75 implementation strategies within four phases and 10 domains and begins to validate the framework. Standard use of strategy names is foundational to compare and understand when implementation strategies are effective, in what dose, for which topics, by whom, and in what context.
A pilot randomized controlled trial of the shogi-assisted cognitive behavioral therapy (S-CBT) preventive stress management program
Authors: Furukawa Hirokazu , Noda Shota , Kitashima Chiho , Omine Manami , Fukumoto Takumi , Ono Hitomi , Ohara Aya , Nakao Mutsuhiro
Published: January 2022

Background

Shogi is a traditional board game in Japan. A preventive stress management program based on Shogi-assisted cognitive behavioral therapy (S-CBT) was applied in the Japanese municipality of Kakogawa City. The study aimed to develop an S-CBT preventive stress management program for the elderly and determine its efficacy.

Methods

The participants were 67 elderly men with amateur-level Shogi skills. They were randomly assigned to either the S-CBT group (n = 33) or the waiting-list control group (n = 34). The S-CBT program was conducted over six 90-min sessions. The outcome measures were recorded using K6 instrument, the Japanese version of the abbreviated Lubben Social Network Scale, five items on cognitive behavioral functioning, and subjective well-being scale.

Results and conclusions

The dropout rates of the S-CBT group and waiting-list control groups were 36.4 and 44.1%, respectively. Effect sizes (Cohen’s d) and 95% confidence intervals (CIs) were calculated for each group. Domains that changed immediately after the S-CBT intervention were problem-solving skills, self-reinforcement, and negative automatic thoughts. Future research should promote mental and physical health through the design of intervention programs using familiar materials.

Trial registration

University Hospital Medical Information Network (UMIN CTR) UMIN000036003.
SUMOylation inhibition enhances dexamethasone sensitivity in multiple myeloma
Authors: Du Li , Liu Wei , Aldana-Masangkay Grace , Pozhitkov Alex , Pichiorri Flavia , Chen Yuan , Rosen Steven T.
Published: January 2022

Background

Multiple myeloma (MM) is an incurable plasma cell malignancy. Although Dexamethasone (Dex) is the most widely used therapeutic drug in MM treatment, patients develop Dex resistance leading to progressive disease, demanding an urgent need to investigate the mechanisms driving Dex resistance and develop new reagents to address this problem. We propose SUMOylation as a potential mechanism regulating Dex resistance and SUMOylation inhibition can enhance Dex sensitivity in MM.

Methods

Using MM cell lines and primary MM samples from relapsing MM patients, we evaluated the effects of knockdown of SUMO E1 (SAE2) or using TAK-981, a novel and specific SUMO E1 inhibitor, on Dex sensitivity. Xenograft mouse models were generated to determine the in vivo anti-MM effects of TAK-981 as a single agent and in combination with Dex. miRNA-seq, RNA-seq and GSEA analysis were utilized for evaluating key factors mediating Dex resistance. Chromatin immunoprecipitation (ChIP) assay was performed to determine the binding occupancy of c-Myc on promoter region of miRs.

Results

We observed a significant negative correlation between SUMO E1 (SAE2) expression and Dex sensitivity in primary MM samples. Knockdown of SAE2 or using TAK-981 significantly enhances myeloma sensitivity to Dex in MM cell lines. Moreover, the enhanced anti-MM activity by TAK-981 and Dex combination has been validated using primary relapsing MM patient samples and xenograft mouse models. SUMOylation inhibition increased glucocorticoid receptor (GR) expression via downregulation miR-130b. Using RNA and microRNA sequencing, we identified miR-551b and miR-25 as important miRs mediating Dex resistance in MM. Overexpression of miR-551b and miR-25 caused resistance to Dex, however, knockdown of miR-551b and miR-25 significantly enhanced Dex sensitivity in MM. SAE2 knockdown or TAK-981 treatment downregulated the expression of miR-551b and miR-25, leading to induction of miR targets ZFP36, ULK1 and p27, resulting in apoptosis and autophagy. We demonstrated c-Myc as a major transcriptional activator of miR-130b, miR-551b and miR-25 and SUMOylation inhibition downregulates these miRs level by decreasing c-Myc level.

Conclusion

Our study proves SUMOylation plays a crucial role in Dex resistance in MM and SUMOylation inhibition appears to be an attractive strategy to advance to the clinic for MM patients.
Respiratory effects of lung recruitment maneuvers depend on the recruitment-to-inflation ratio in patients with COVID-19-related acute respiratory distress syndrome
Authors: Zerbib Yoann , Lambour Alexis , Maizel Julien , Kontar Loay , De Cagny Bertrand , Soupison Thierry , Bradier Thomas , Slama Michel , Brault Clément
Published: January 2022

Background

In the context of acute respiratory distress syndrome (ARDS), the response to lung recruitment maneuvers (LRMs) varies considerably from one patient to another and so is difficult to predict. The aim of the study was to determine whether or not the recruitment-to-inflation (R/I) ratio could differentiate between patients according to the change in lung mechanics during the LRM.

Methods

We evaluated the changes in gas exchange and respiratory mechanics induced by a stepwise LRM at a constant driving pressure of 15 cmH2O during pressure-controlled ventilation. We assessed lung recruitability by measuring the R/I ratio. Patients were dichotomized with regard to the median R/I ratio.

Results

We included 30 patients with moderate-to-severe ARDS and a median [interquartile range] R/I ratio of 0.62 [0.42–0.83]. After the LRM, patients with high recruitability (R/I ratio ≥ 0.62) presented an improvement in the PaO2/FiO2 ratio, due to significant increase in respiratory system compliance (33 [27–42] vs. 42 [35–60] mL/cmH2O; p < 0.001). In low recruitability patients (R/I < 0.62), the increase in PaO2/FiO2 ratio was associated with a significant decrease in pulse pressure as a surrogate of cardiac output (70 [55–85] vs. 50 [51–67] mmHg; p = 0.01) but not with a significant change in respiratory system compliance (33 [24–47] vs. 35 [25–47] mL/cmH2O; p = 0.74).

Conclusion

After the LRM, patients with high recruitability presented a significant increase in respiratory system compliance (indicating a gain in ventilated area), while those with low recruitability presented a decrease in pulse pressure suggesting a drop in cardiac output and therefore in intrapulmonary shunt.
Effects of host vimentin on Eimeria tenella sporozoite invasion
Authors: Liu Zhan , Geng Xiangfei , Zhao Qiping , Zhu Shunhai , Han Hongyu , Yu Yu , Huang Wenhao , Yao Yawen , Huang Bing , Dong Hui
Published: January 2022

Background

Chicken coccidiosis is a parasitic disease caused byEimeriaof Apicomplexa, which has caused great economic loss to the poultry breeding industry. Host vimentin is a key protein in the process of infection of many pathogens. In an earlier phosphorylation proteomics study, we found that the phosphorylation level of host vimentin was significantly regulated afterEimeria tenellasporozoite infection. Therefore, we explored the role of host vimentin in the invasion of host cells by sporozoites.

Methods

Chicken vimentin protein was cloned and expressed. We used qPCR, western blotting, and indirect immunofluorescence to detect levels of mRNA transcription, translation, and phosphorylation, and changes in the distribution of vimentin afterE. tenellasporozoite infection. The sporozoite invasion rate in DF-1 cells treated with vimentin polyclonal antibody or with small interfering RNA (siRNA), which downregulated vimentin expression, was assessed by an in vitro invasion test.

Results

The results showed that vimentin transcription and translation levels increased continually at 6–72 h afterE. tenellasporozoite infection, and the total phosphorylation levels of vimentin also changed. About 24 h after sporozoite infection, vimentin accumulated around sporozoites in DF-1 cells. Treating DF-1 cells with vimentin polyclonal antibody or downregulating vimentin expression by siRNA significantly improved the invasion efficiency of sporozoites.

Conclusion

In this study, we showed that vimentin played an inhibitory role during the invasion of sporozoites. These data provided a foundation for clarifying the relationship betweenEimeriaand the host.

Graphical Abstract
A discrepant presentation of bacteremia in the emergency department linked to a Fusobacterium nucleatum infection: a case report
Authors: Michel Johnny , Joly Luc-Marie , Lvovschi Virginie Eve
Published: January 2022

Background

Fusobacterium nucleatum is an anaerobic bacterium mainly responsible for acute or chronic infection of the ear, nose, and throat, potentially bacteremic with a risk of extraoral metastatic infection. Bacteremia occurs mainly in the elderly or in immunodeficient individuals, with high mortality. F. nucleatum is not the first cause of tonsillar infection in emergency departments, which are more often the consequence of a viral or streptococcal infection, but it is a risk factor for severe bacterial infection, especially in a viral pandemic context.

Case presentation

A 25-year-old European woman with no history presented to the emergency department with fever (38.9 °C), pharyngeal symptoms, intermittent headaches, and alteration of general condition. On examination, she presented odynophagia associated with moderate tonsillar hypertrophy, her neck was painful but flexible. A rapid diagnostic test for beta-hemolytic group streptococcus was negative. First biological analyses revealed an inflammatory syndrome with C-reactive protein of 76 mg/L. Procalcitonin was measured secondarily, and was 2.16 µg/L. Faced with discordant clinical and biological findings, a lumbar puncture was performed, which came back negative. At hour eight, hypotension was observed but corrected after filling with physiological serum. The patient was hospitalized for monitoring, based on a hypothesis of severe viral presentation. At hour 24, pyrexia confirmed this hypothesis. A spontaneous but transient improvement and no new hemodynamic event led to early discharge. At day three, she was rehospitalized for increased and continuous headaches, without hemodynamic severity. A broad-spectrum probabilistic antibiotic therapy of ceftriaxone and metronidazole was started due to first blood cultures positive for anaerobic Gram-negative bacilli, while waiting for identification of the pathogen. Three days later, F. nucleatum was identified. According to the microbiological results, antibiotic therapy was adapted with amoxicillin and clavulanic acid, and no further complications were observed during clinical or complementary examinations. The final diagnosis was a F. nucleatum oropharyngeal infection complicated by bacteremia, without metastatic spread.

Conclusion

The etiologies of tonsillar infection are not limited to benign viruses or bacteria. These should not be overlooked in emergency medicine, especially when the clinical presentation is discrepant. A combination of early bacterial investigations as blood culture and close clinical monitoring is the only safe way to detect bacteremia, especially in immunocompetent patients.
Auxiliary function-based algorithm for blind extraction of a moving speaker
Authors: Janský Jakub , Koldovský Zbyněk , Málek Jiří , Kounovský Tomáš , Čmejla Jaroslav
Published: January 2022

In this paper, we propose a novel algorithm for blind source extraction (BSE) of a moving acoustic source recorded by multiple microphones. The algorithm is based on independent vector extraction (IVE) where the contrast function is optimized using the auxiliary function-based technique and where the recently proposed constant separating vector (CSV) mixing model is assumed. CSV allows for movements of the extracted source within the analyzed batch of recordings. We provide a practical explanation of how the CSV model works when extracting a moving acoustic source. Then, the proposed algorithm is experimentally verified on the task of blind extraction of a moving speaker. The algorithm is compared with state-of-the-art blind methods and with an adaptive BSE algorithm which processes data in a sequential manner. The results confirm that the proposed algorithm can extract the moving speaker better than the BSE methods based on the conventional mixing model and that it achieves improved extraction accuracy than the adaptive method.
Unsung voices of technology in school education-findings using the constructivist grounded theory approach
Authors: Deepa V. , Sujatha R. , Mohan Jitendra
Published: January 2022

Technology adoption for school education further gained momentum during the COVID-19 pandemic. However, the challenges and strategies of children belonging to the less privileged (we use ‘privileged’ in the article to identify those enjoying a standard of living or rights as majority of people in the society) families are different from those of the children who come from socio-economically better-off (privileged) backgrounds. The purpose of this research is to explore the experiences of children with school education and using technology for learning. Past studies have highlighted the use of internet and communication technologies as a promising solution to provide quality school education in the remotest parts of the country. Previous research has also ascertained that the socio-economic status divide has no significant impact on the students’ ability to learn using technology. Children can use technology to learn irrespective of their socio-economic status and background. We conducted this exploratory qualitative study from a constructivist grounded theory perspective. A purposive sample of 14 students (9 from underprivileged and 5 from privileged family backgrounds) in the age group of 6–14 years, was used and unstructured interviews were conducted. We analysed the data using constructivist grounded theory methodology. We found that the experiences of privileged and underprivileged children differed with respect to access to internet, affordability of ICT device, quality teachers, parental support, and financial sponsorship. However, the experiences and perspectives of the children were found to be similar with respect to personal ownership of mobile phone device for unlimited time at own disposal, self-directed learning and having a trusted study advisor. The findings may be useful to policy makers and EdTech firms to build strategies and solutions for effective implementation of universal school education in the country.
On the use of ELF/VLF emissions triggered by HAARP to simulate PLHR and to study associated MLR events
Authors: Parrot Michel , Němec Frantisěk , Cohen Morris B. , Gołkowski Mark
Published: January 2022

A spectrogram of Power Line Harmonic Radiation (PLHR) consists of a set of lines with frequency spacing corresponding exactly to 50 or 60 Hz. It is distinct from a spectrogram of Magnetospheric Line Radiation (MLR) where the lines are not equidistant and drift in frequency. PLHR and MLR propagate in the ionosphere and the magnetosphere and are recorded by ground experiments and satellites. If the source of PLHR is evident, the origin of the MLR is still under debate and the purpose of this paper is to understand how MLR lines are formed. The ELF waves triggered by High-frequency Active Auroral Research Program (HAARP) in the ionosphere are used to simulate lines (pulses of different lengths and different frequencies). Several receivers are utilized to survey the propagation of these pulses. The resulting waves are simultaneously recorded by ground-based experiments close to HAARP in Alaska, and by the low-altitude satellite DEMETER either above HAARP or its magnetically conjugate point. Six cases are presented which show that 2-hop echoes (pulses going back and forth in the magnetosphere) are very often observed. The pulses emitted by HAARP return in the Northern hemisphere with a time delay. A detailed spectral analysis shows that sidebands can be triggered and create elements with superposed frequency lines which drift in frequency during the propagation. These elements acting like quasi-periodic emissions are subjected to equatorial amplification and can trigger hooks and falling tones. At the end all these known physical processes lead to the formation of the observed MLR by HAARP pulses. It is shown that there is a tendency for the MLR frequencies of occurrence to be around 2 kHz although the exciting waves have been emitted at lower and higher frequencies.

Graphical Abstract
Precise aftershock distribution of the 2019 Yamagata-oki earthquake using newly developed simple anchored-buoy ocean bottom seismometers and land seismic stations
Authors: Shinohara Masanao , Sakai Shin’ichi , Okada Tomomi , Sato Hiroshi , Yamashita Yusuke , Hino Ryota , Mochizuki Kimihiro , Akuhara Takeshi
Published: January 2022

An earthquake with a magnitude of 6.7 occurred in the Japan Sea off Yamagata on June 18, 2019. The mainshock had a source mechanism of reverse-fault type with a compression axis of WNW–ESE direction. Since the source area is positioned in a marine area, seafloor seismic observation is indispensable for obtaining the precise distribution of the aftershocks. The source area has a water depth of less than 100 m, and fishing activity is high. It is difficult to perform aftershock observation using ordinary free-fall pop-up type ocean bottom seismometers (OBSs). We developed a simple anchored-buoy type OBS for shallow water depths and performed the seafloor observation using this. The seafloor seismic unit had three-component seismometers and a hydrophone. Two orthogonal tiltmeters and an azimuth meter monitored the attitude of the package. For seismic observation at shallow water depth, we concluded that an anchored-buoy system would have the advantage of avoiding accidents. Our anchored-buoy OBS was based on a system used in fisheries. We deployed three anchored-buoy OBSs in the source region where the water depth was approximately 80 m on July 5, 2019, and two of the OBSs were recovered on July 13, 2019. Temporary land seismic stations with a three-component seismometer were also installed. The arrival times of P- and S-waves were read from the records of the OBSs and land stations, and we located hypocenters with correction for travel time. A preliminary location was performed using absolute travel time and final hypocenters were obtained using the double-difference method. The aftershocks were distributed at a depth range of 2.5 km to 10 km and along a plane dipping to the southeast. The plane formed by the aftershocks is consistent with the focal mechanism of the mainshock. The activity region of the aftershocks was positioned in the upper part of the upper crust. Focal mechanisms were estimated using the polarity of the first arrivals. Although many aftershocks had a reverse-fault focal mechanism similar to the focal solution of the mainshock, normal-fault type and strike–slip fault type focal mechanisms were also estimated.

Graphical Abstract
Relationship between topography, tropospheric wind, and frequency of mountain waves in the upper mesosphere over the Kanto area of Japan
Authors: Ishii Satoshi , Tomikawa Yoshihiro , Okuda Masahiro , Suzuki Hidehiko
Published: January 2022

Imaging observations of OH airglow were performed at Meiji University, Japan (35.6° N, 139.5° E), from May 2018 to December 2019. Mountainous areas are located to the west of the imager, and westerly winds are dominant in the lower atmosphere throughout the year. Mountain waves (MWs) are generated and occasionally propagate to the upper atmosphere. However, only four likely MW events were identified, which are considerably fewer than expected. There are two possible reasons for the low incidence: (1) MWs do not propagate easily to the upper mesosphere due to background wind conditions, and/or (2) the frequency of MW excitation was low around the observation site. Former possibility is found not to be a main reason to explain the frequency by assuming typical wind profiles in troposphere and upper mesosphere over Japan. Thus, frequency and spatial distribution of orographic wavy clouds were investigated by analyzing images taken by the Himawari-8 geostationary meteorological satellite in 2018. The number of days when wavy clouds were detected in the troposphere around the observation site (Kanto area) was about a quarter of that around the Tohoku area. This result indicates that frequency of over-mountain flow which is thought to be a source of excitation of MWs is low in Kanto area. We also found that the angle between the horizontal wind direction in troposphere and the orientation of the mountain ridge is a good proxy for the occurrence of orographic wavy clouds, i.e., excitation of MWs. We applied this proxy to the topography around the world to investigate regions where MWs are likely to be excited frequently throughout the year to discuss the likelihood of "MW hotspots" at various spatial scale.

Graphical Abstract
An event study on broadband electric field noises and electron distributions in the lunar wake boundary
Authors: Nishino Masaki N. , Kasahara Yoshiya , Harada Yuki , Saito Yoshifumi , Tsunakawa Hideo , Kumamoto Atsushi , Yokota Shoichiro , Takahashi Futoshi , Matsushima Masaki , Shibuya Hidetoshi , Shimizu Hisayoshi , Miyashita Yukinaga , Goto Yoshitaka , Ono Takayuki
Published: January 2022

Wave–particle interactions are fundamental processes in space plasma, and some plasma waves, including electrostatic solitary waves (ESWs), are recognised as broadband noises (BBNs) in the electric field spectral data. Spacecraft observations in recent decades have detected BBNs around the Moon, but the generation mechanism of the BBNs is not fully understood. Here, we study a wake boundary traversal with BBNs observed by Kaguya, which includes an ESW event previously reported by Hashimoto et al. Geophys Res Lett 37:L19204 10.1029/2010GL044529 (2010). Focusing on the relation between BBNs and electron pitch-angle distribution functions, we show that upward electron beams from the nightside lunar surface are effective for the generation of BBNs, in contrast to the original interpretation by Hashimoto et al. Geophys Res Lett 37:L19204 10.1029/2010GL044529 (2010) that high-energy electrons accelerated by strong ambipolar electric fields excite ESWs in the region far from the Moon. When the BBNs were observed by the Kaguya spacecraft in the wake boundary, the spacecraft’s location was magnetically connected to the nightside lunar surface, and bi-streaming electron distributions of downward-going solar wind strahl component and upward-going field-aligned beams (at $$\sim$$
∼
124 eV) were detected. The interplanetary magnetic field was dominated by a positive $$B_Z$$

B
Z

(i.e. the northward component), and strahl electrons travelled in the antiparallel direction to the interplanetary magnetic field (i.e. southward), which enabled the strahl electrons to precipitate onto the nightside lunar surface directly. The incident solar wind electrons cause negative charging of the nightside lunar surface, which generates downward electric fields that accelerate electrons from the nightside surface toward higher altitudes along the magnetic field. The bidirectional electron distribution is not a sufficient condition for the BBN generation, and the distribution of upward electron beams seems to be correlated with the BBNs. Ambipolar electric fields in the wake boundary should also contribute to the electron acceleration toward higher altitudes and further intrusion of the solar wind ions into the deeper wake. We suggest that solar wind ion intrusion into the wake boundary is also an important factor that controls the BBN generation by facilitating the influx of solar wind electrons there.

Graphical Abstract
Repeatability and reproducibility of corneal higher-order aberrations measurements after small incision lenticule extraction using the Scheimpflug-Placido topographer
Authors: Ning Rui , Gao Rongrong , Piñero David P. , Zhang Jun , Gao Qingyi , Jin Yili , Wang Yiran , Wang Chenxiao , Huang Jinhai
Published: January 2022

Background

To evaluate the precision of corneal higher-order aberrations measurements after small incision lenticule extraction (SMILE) using the Sirius Scheimpflug-Placido topographer (CSO, Italy).

Methods

Seventy-five eyes from 75 postoperative subjects were included in this prospective study. Three consecutive corneal aberrometric measurements were obtained with the Scheimpflug-Placido topographer by two experienced operators to assess intra- and inter-observer reproducibility. The within-subject standard deviation (Sw), test-retest repeatability (TRT) and the intraclass correlation coefficient (ICC) were calculated.

Results

For intraobserver repeatability of anterior and total corneal aberrations, all ICCs were more than 0.922, except for trefoil (0.722 to 0.768). The ICCs of total root mean square (RMS), coma Z (3, ± 1), and spherical aberration Z (4, 0) were over 0.810 while higher-order RMS, trefoil Z (3, ± 3), and astigmatism II Z (4, ± 2) were below 0.634 for posterior corneal surface aberrations. All Sw values for all types of aberrations were equal to or below 0.07 μm. Regarding interobserver reproducibility, all TRT values were no more than 0.12 μm, 0.05 μm, and 0.11 μm for anterior, posterior, and total corneal aberrations, respectively. The ICC values ranged from 0.875 to 0.989, from 0.686 to 0.976 and over 0.834 for anterior, posterior, and total corneal aberrations, respectively.

Conclusions

The repeatability of measurements of anterior and total corneal aberrations with the Sirius system in corneas after SMILE surgery was high, except for trefoil. There was some variability in posterior corneal aberrometric measurements. High reproducibility of corneal aberrometric measurements was observed between measurements of both examiners, except for trefoil, with poor to moderate reproducibility.
Smooth muscle degeneration of the mesenteric and branching veins causing ischemic enteritis: a case report
Authors: Sunakawa Taiki , Ito Nobuo , Moriyasu Ryo , Seki Nobuya , Takeuchi Daisuke , Sasahara Kotaro
Published: January 2022

Background

Ischemic bowel injuries are generally caused by arteriosclerosis, thromboembolism, or vasculitis. Ischemic enteritis is less common than ischemic colitis because of the rich collateral arteries of the small intestine. In the present case, smooth muscle degeneration of the mesenteric to the submucosal veins caused ischemic enteritis and small bowel obstruction.

Case presentation

An 85-year-old woman with recurrent enteritis eventually developed small bowel obstruction. We performed laparoscopic partial resection of the small intestine. The pathological findings revealed smooth muscle degeneration of the mesenteric veins that caused ischemic enteritis. Venous changes were detected not only in the injured region, but also in a part of the normal region of the resected specimen. She continued to experience some minor symptoms postoperatively; however, these symptoms subsided in a short period with medicine discontinuation.

Conclusion

This report shows the possibility that a disease causes ischemic enteritis with unique venous pathological changes and may recur postoperatively.
Two-time perforation of the ileal J-pouch 6 and 18 years after restorative proctocolectomy and ileal pouch–anal anastomosis for familial adenomatous polyposis: a case report
Authors: Shibata Kengo , Ebinuma Shota , Sakamoto Sodai , Suzuki Asami , Terasaki Yasunobu , Taketomi Akinobu
Published: January 2022

Background

Perforation of the ileal J-pouch after restorative proctocolectomy and ileal pouch–anal anastomosis are extremely rare. There has been no report of perforation of the ileal J-pouch occurring twice over several years. We report the first case of perforation at 6 and 18 years following restorative proctocolectomy.

Case presentation

The patient was a 52-year-old man who underwent a two-stage restorative proctocolectomy with a hand-sewn ileal J-pouch anal anastomosis due to familial adenomatous polyposis and sigmoid colon cancer at 34 years of age. At the age of 40, he underwent ileal pouch resection at its blind end, abdominal drainage, and anastomotic dilatation. The patient had a perforation of the blind end of the ileal J-pouch from increased intraluminal pressure, with anastomotic stricture and pervasive peritonitis. The patient had no symptoms for a few years; however, 18 years after the initial surgery and 12 years after the first perforation, the patient presented with severe abdominal pain. Computed tomography demonstrated pneumoperitoneum; accordingly, laparotomy was performed. Upon opening the abdominal cavity, contaminated ascites and inflammatory changes were documented involving the ileum. A 2-mm perforation involving the blind end of the ileal J-pouch was also observed and repaired, followed by temporary loop ileostomy creation. Postoperative endoscopy revealed an ulcer in the ileal J-pouch and a stricture located directly at the anastomosis.

Conclusions

The blind end of the J-pouch repeatedly perforated over the years due to recurrent anastomotic stricture. Regular surveillance is, therefore, considered necessary for the release of stricture, maintenance of anastomotic patency, and prevention of ileal J-pouch perforation.
Facilitation of colonic T cell immune responses is associated with an exacerbation of dextran sodium sulfate–induced colitis in mice lacking microsomal prostaglandin E synthase-1
Authors: Kojima Fumiaki , Sekiya Hiroki , Hioki Yuka , Kashiwagi Hitoshi , Kubo Makoto , Nakamura Masaki , Maehana Shotaro , Imamichi Yoshitaka , Yuhki Koh-ichi , Ushikubi Fumitaka , Kitasato Hidero , Ichikawa Takafumi
Published: January 2022

Background

Microsomal prostaglandin E synthase-1 (mPGES-1) is a key enzyme that acts downstream of cyclooxygenase and plays a major role in inflammation by converting prostaglandin (PG) H2to PGE2. The present study investigated the effect of genetic deletion of mPGES-1 on the development of immunologic responses to experimental colitis induced by dextran sodium sulfate (DSS), a well-established model of inflammatory bowel disease (IBD).

Methods

Colitis was induced in mice lacking mPGES-1 (mPGES-1−/−mice) and wild-type (WT) mice by administering DSS for 7 days. Colitis was assessed by body weight loss, diarrhea, fecal bleeding, and histological features. The colonic expression of mPGES-1 was determined by real-time PCR, western blotting, and immunohistochemistry. The impact of mPGES-1 deficiency on T cell immunity was determined by flow cytometry and T cell depletionin vivo.

Results

After administration of DSS, mPGES-1−/−mice exhibited more severe weight loss, diarrhea, and fecal bleeding than WT mice. Histological analysis further showed significant exacerbation of colonic inflammation in mPGES-1−/−mice. In WT mice, the colonic expression of mPGES-1 was highly induced on both mRNA and protein levels and colonic PGE2increased significantly after DSS administration. Additionally, mPGES-1 protein was localized in the colonic mucosal epithelium and infiltrated inflammatory cells in underlying connective tissues and the lamina propria. The abnormalities consistent with colitis in mPGES-1−/−mice were associated with higher expression of colonic T-helper (Th)17 and Th1 cytokines, including interleukin 17A and interferon-γ. Furthermore, lack of mPGES-1 increased the numbers of Th17 and Th1 cells in the lamina propria mononuclear cells within the colon, even though the number of suppressive regulatory T cells also increased. CD4+T cell depletion effectively reduced symptoms of colitis as well as colonic expression of Th17 and Th1 cytokines in mPGES-1−/−mice, suggesting the requirement of CD4+T cells in the exacerbation of DSS-induced colitis under mPGES-1 deficiency.

Conclusions

These results demonstrate that mPGES-1 is the main enzyme responsible for colonic PGE2production and deficiency of mPGES-1 facilitates the development of colitis by affecting the development of colonic T cell–mediated immunity. mPGES-1 might therefore impact both the intestinal inflammation and T cell–mediated immunity associated with IBD.
Adding a fatigue item to the EQ-5D-5L improves its psychometric performance in the general population
Authors: Spronk Inge , Polinder Suzanne , Bonsel Gouke J. , Janssen M. F. , Haagsma Juanita A.
Published: January 2022

Background

Fatigue is a common and often disturbing sequela of serious chronic health conditions. In the widely applied HRQL instrument, the EQ-5D, this aspect is not included directly, for its assumed lack of additional information. We investigated the validity of this assumption by determining the gain—if any—of an additional fatigue item to the EQ-5D-5L in a general population sample.

Methods

A Dutch general population sample (including diseased people) completed a web-based survey including the EQ-5D-5L and the Rivermead Post-Concussion Symptoms Questionnaire (RPQ). The RPQ fatigue item was used to create the EQ-5D-5L + Fatigue. We head-to-head compared the psychometric performance contrasting the EQ-5D-5L and EQ-5D-5L + Fatigue: distribution (e.g. ceiling), informativity cf. Shannon's indices, convergent validity, domain dependency, and explanatory power. Results were compared between subgroups with and without ≥ 1 chronic health condition.

Results

The study population consisted of 3027 persons of whom 52% had a chronic health condition. The mean EQ-5D-5L utility score was 0.83 and 48% experienced some degree of fatigue. Adding the fatigue item to the EQ-5D-5L decreased the ceiling effect, increased absolute informativity (Hʹ = 6.44 vs. Hʹ = 4.90) and relative informativity (Jʹ = 0.46 vs. Jʹ = 0.42). The extra fatigue item slightly increased convergent validity (Spearman’s rank correlation coefficient = − 0.61 vs. − 0.62). Domain dependency analysis showed that all EQ-5D-5L domains are dominant over the fatigue item. Explanatory power of the EQ-5D-5L + Fatigue was higher compared to the EQ-5D-5L (R2 = 0.42 vs. 0.39). The gain is substantially larger in the subgroup with chronic health conditions.

Conclusions

Adding a fatigue item to the EQ-5D-5L improved all psychometric performance criteria of the enriched instrument in the general population. Effects are substantially larger in the subgroup with chronic health conditions, indicating that adding a fatigue item to the EQ-5D-5L is especially relevant in evaluating the HRQL of diseased people.
Examining the role of astrogliosis and JNK signaling in post-traumatic epilepsy
Authors: Small Coulter , Dagra Abeer , Martinez Melanie , Williams Eric , Lucke-Wold Brandon
Published: January 2022

Objective

Post-traumatic epilepsy is a devastating complication of traumatic brain injury that has no targeted pharmacological therapy. Previous literature has explored the role of the c-Jun N-terminal kinase (JNK) pathway in epilepsy and the creation of epileptogenic foci by reactive astrogliosis; however, the relationship between reactive astrogliosis and the c-Jun N-terminal kinase signaling pathway in the development of post-traumatic epilepsy has not been thoroughly examined.

Methods

Four experimental groups, consisting of c57/b16 male mice, were examined: (1) control, (2) traumatic brain injury of graded severity (mild, moderate, severe), (3) sub-convulsive kainic acid alone without traumatic brain injury (15 mg/kg i.p.), and (4) sub-convulsive kainic acid administered 72 h after moderate traumatic brain injury. Modified Racine scale from 1 to 72 h and total beam breaks at 72 h were used to assess seizure activity. Immunohistochemistry and western blot were utilized to examine astrogliosis (GFAP), microglia activation (IBA-1), and phosphorylated JNK in prefrontal cortex samples collected from the contracoup side at 72 h post-injury.

Results

Astrogliosis, measured by GFAP, was increased after traumatic brain injury and increased commensurately based on the degree of injury. Mice with traumatic brain injury demonstrated a four-fold increase in phosphorylated JNK: p < 0.001. Sub-convulsive kainic acid administration did not increase seizure activity nor phosphorylation of JNK in mice without traumatic brain injury; however, sub-convulsive kainic acid administration in mice with moderate traumatic brain injury did increase phosphorylated JNK. Seizure activity was worse in mice, with traumatic brain injury, administered kainic acid than mice administered kainic acid.

Conclusions

Reactive astrocytes may have dysfunctional glutamate regulation causing an increase in phosphorylated JNK after kainic acid administration. Future studies exploring the effects of JNK inhibition on post-traumatic epilepsy are recommended.
UK Medical Cannabis Registry palliative care patients cohort: initial experience and outcomes
Authors: Nimalan Devaki , Kawka Michal , Erridge Simon , Ergisi Mehmet , Harris Michael , Salazar Oliver , Ali Rayyan , Loupasaki Katerina , Holvey Carl , Coomber Ross , Platt Michael , Rucker James J. , Khan Shaheen , Sodergren Mikael H.
Published: January 2022

Introduction

Palliative care aims to improve quality of life through optimal symptom control and pain management. Cannabis-based medicinal products (CBMPs) have a proven role in the treatment of chemotherapy-induced nausea and vomiting. However, there is a paucity of high-quality evidence with regards to the optimal therapeutic regimen, safety, and effectiveness of CBMPs in palliative care, as existing clinical trials are limited by methodological heterogeneity. The aim of this study is to summarise the outcomes of the initial subgroup of patients from the UK Medical Cannabis Registry who were prescribed CBMPs for a primary indication of palliative care, cancer pain and chemotherapy-induced nausea and vomiting, including effects on health-related quality of life and clinical safety.

Methods

A case series from the UK Medical Cannabis Registry of patients, who were receiving CBMPs for the indication of palliative care was undertaken. The primary outcome consisted of changes in patient-reported outcome measures including EQ-5D-5L, General Anxiety Disorder-7 (GAD-7), Single-Item Sleep Quality Scale (SQS), Pain Visual Analog Scale (VAS) and the Australia-Modified Karnofsky Performance Scale at 1 and 3 months compared to baseline. Secondary outcomes included the incidence and characteristics of adverse events. Statistical significance was defined by p-value< 0.050.

Results

Sixteen patients were included in the analysis, with a mean age of 63.25 years. Patients were predominantly prescribed CBMPs for cancer-related palliative care (n = 15, 94%). The median initial CBD and THC daily doses were 32.0 mg (Range: 20.0–384.0 mg) and 1.3 mg (Range: 1.0–16.0 mg) respectively. Improvements in patient reported health outcomes were observed according to SQS, EQ-5D-5L mobility, pain and discomfort, and anxiety and depression subdomains, EQ-5D-5L index, EQ-VAS and Pain VAS validated scales at both 1-month and 3-months, however, the changes were not statistically significant. Three adverse events (18.75%) were reported, all of which were either mild or moderate in severity.

Conclusion

This small study provides an exploratory analysis of the role of CBMPs in palliative care in the first cohort of patients since CBMPs legalisation in the UK. CBMPs were tolerated with few adverse events, all of which were mild or moderate and resolved spontaneously. Further long-term safety and efficacy studies involving larger cohorts are needed to establish CBMPs role in palliative care, including comparisons with standard treatments.
Improvement of drought tolerance in rice using line X tester mating design and biochemical molecular markers
Authors: El-Mouhamady Almoataz Bellah Ali , Gad Abdul Aziz M. , Karim Ghada S. A. Abdel
Published: January 2022

Background

Water stress, specifically the limited water resources needed to grow strategic crops, especially rice, poses a great threat to crop productivity. So, it was imperative that scientists all work together to try genetically improving the rice for drought tolerance in light of these environmental challenges. The aim of this study is trying to know the genetic behavior responsible for water-deficit tolerance in rice genotypes but at the molecular level. Moreover, this attempt will be an important leap in the process of genetic improvement in rice for water stress tolerance in Egypt.

Results

Twenty-three rice genotypes including eight parents and their fifteen F1 crosses or (the first hybrid generation) by line X tester analysis were evaluated for water stress tolerance during two experiments (the control and drought experiment) besides some molecular–biochemical studies for eight parents and the highest selected five crosses for water stress tolerance. The research revealed that five rice crosses out of fifteen hybrids were highly tolerant to water stress compared to the normal conditions. Data of biochemical markers indicated the presence of bands that are considered as molecular genetic markers for water-deficit tolerance in some rice genotypes, and this is the scientific progress achieved in this research. This was evident by increasing the density and concentration of SDS-protein electrophoresis besides enhancing the activities of peroxidase (POD) and polyphenol oxidase (PPO) under water-deficit conditions, which confirmed the tolerance of drought stress in the eight rice genotypes and the best five crosses from the first hybrid generation.

Conclusion

The five promising and superior rice hybrids showed an unparalleled tolerance to water stress in all evaluated traits under water stress treatment compared to the standard experiment. Also, biochemical and molecular parameters evidence confirmed the existence of unquestionable evidence that it represents the main nucleus for producing rice lines tolerated for drought stress under Egyptian conditions.
In vitro assessment of deworming potential of Guiera senegalensis in Nigerian ethnoveterinary industry using Caenorhabditis elegans
Authors: Gagman Haladu Ali , Ahmad Hamdan , Him Nik Ahmad Irwan Izzaudin Nik , Avicor Silas Wintuma
Published: January 2022

Background

Although Guiera senegalensis is used as a dewormer in ethnoveterinary health care in Nigeria, its anthelmintic potential has not been validated. Hence, this work investigated the in vitro anthelmintic potential of G. senegalensis extracts on two Caenorhabditis elegans strains: Bristol N2 (wild type/ivermectin susceptible) and DA1316 (ivermectin resistant).

Results

Aqueous and methanol extracts of G. senegalensis were tested against the motility of the L4 larvae at two exposure periods of 24 and 48 h and found to be active against the C. elegans strains. Motility of C. elegans DA1316 was reduced to 18.6% and 8.3% by aqueous and methanol extracts, respectively, at 2.0 mg/ml after 48 h, whereas that of C. elegans DA1316 treated with ivermectin (0.02 µg/ml) remained above 95%. The motility of C. elegans Bristol N2 was reduced to 16.6% and 7.2% by aqueous and methanol extracts, respectively, at 2.0 mg/ml after 48 h and ≤ 2.7% by ivermectin (0.02 µg/ml). Activity of the plant extracts was concentration and time dependent.

Conclusions

This work confirms the anthelmintic activity of G. senegalensis and its effectiveness against ivermectin-resistant nematodes, thus validating its ethnoveterinary use as an animal dewormer in Nigeria and pharmacological potential as a source of anthelmintic compounds against ivermectin-resistant nematodes. There is, however, the need for in vivo studies to confirm the in vitro efficacy of the extracts.