bluuebunny/crossref_metadata_2025 huggingface dataset:
DC_OCEAN: an open-source algorithm for identification of duplicates in ocean databases
Authors: Song Xinyi , Tan Zhetao , Locarnini Ricardo , Simoncelli Simona , Cowley Rebecca , Kizu Shoichi , Boyer Tim , Reseghetti Franco , Castelao Guilherme , Gouretski Viktor , Cheng Lijing
Published: October 2024

A high-quality hydrographic observational database is essential for ocean and climate studies and operational applications. Because there are numerous global and regional ocean databases, duplicate data continues to be an issue in data management, data processing and database merging, posing a challenge on effectively and accurately using oceanographic data to derive robust statistics and reliable data products. This study aims to provide algorithms to identify the duplicates and assign labels to them. We propose first a set of criteria to define the duplicate data; and second, an open-source and semi-automatic system to detect duplicate data and erroneous metadata. This system includes several algorithms for automatic checks using statistical methods (such as Principal Component Analysis and entropy weighting) and an additional expert (manual) check. The robustness of the system is then evaluated with a subset of the World Ocean Database (WOD18) with over 600,000 in-situ temperature and salinity profiles. This system is an open-source Python package (named DC_OCEAN) allowing users to effectively use the software. Users can customize their settings. The application result from the WOD18 subset also forms a benchmark dataset, which is available to support future studies on duplicate checks, metadata error identification, and machine learning applications. This duplicate checking system will be incorporated into the International Quality-controlled Ocean Database (IQuOD) data quality control system to guarantee the uniqueness of ocean observation data in this product.
Influence of local scour on the dynamic responses of OWTs under wind-wave loads
Authors: Yao Yong , Rao Mumin , Yu Chi , Wu Zhichao , Zhang Cheng , Wu Tianyu
Published: October 2024

Offshore wind turbines (OWTs) often operate in complex marine environments, where they are not only subjected to wind and wave loads, but also adversely affected by scour. Therefore, it is of great significance to explore the effect of scour on the dynamic responses of OWTs under external loads to ensure structural safety, improve performance, and extend service life. In this study, a comprehensive numerical model of a 5-MW OWT, including tower, monopile, and soil-structure interaction (SSI) systems, is established by using ABAQUS platform. Aerodynamic loads is generated using blade element momentum, while wave loads is generated using the P-M spectral. The depth of scour is obtained based on on-site measured data. A comparative analysis is conducted between fixed foundations and SSI systems when conducting dynamic response analysis of OWTs under wave loads. Subsequently, the effect of scour on dynamic responses of OWTs under aerodynamic and wave loads is investigated. Results demonstrate that SSI can significantly influence the natural frequency and dynamic responses of the OWT. Therefore, it is essential to thoroughly consider SSI when evaluating the dynamic response of the OWT with local scour. The tower-top displacement and acceleration of the OWT with show a significant increasing trend compared to the non-scoured OWT. An increase in scour depth leads to higher maximum stress and stress amplitude in the steel monopile, which could potentially cause fatigue issues and should be given due attention.
A novel metastatic tumor segmentation method with a new evaluation metric in clinic study
Authors: Li Bin , Sun Qiushi , Fang Xianjin , Yang Yang , Li Xiang
Published: October 2024

Background

Brain metastases are the most common brain malignancies. Automatic detection and segmentation of brain metastases provide significant assistance for radiologists in discovering the location of the lesion and making accurate clinical decisions on brain tumor type for precise treatment.

Objectives

However, due to the small size of the brain metastases, existing brain metastases segmentation produces unsatisfactory results and has not been evaluated on clinic datasets.

Methodology

In this work, we propose a new metastasis segmentation method DRAU-Net, which integrates a new attention mechanism multi-branch weighted attention module and DResConv module, making the extraction of tumor boundaries more complete. To enhance the evaluation of both the segmentation quality and the number of targets, we propose a novel medical image segmentation evaluation metric: multi-objective segmentation integrity metric, which effectively improves the evaluation results on multiple brain metastases with small size.

Results

Experimental results evaluated on the BraTS2023 dataset and collected clinical data show that the proposed method has achieved excellent performance with an average dice coefficient of 0.6858 and multi-objective segmentation integrity metric of 0.5582.

Conclusion

Compared with other methods, our proposed method achieved the best performance in the task of segmenting metastatic tumors.
Case report: A case of proliferative glomerulonephritis with monoclonal kappa-light chain deposits treated with daratumumab combination therapy
Authors: Wang Jue , Lv Jun-Ting , Xiao Dan , Liu Jia , Du Jun , Zhong Lu
Published: October 2024

Introduction

Proliferative glomerulonephritis with monoclonal immunoglobulin deposits (PGNMID) is a chronic glomerular disease caused by monoclonal gammopathy. IgG (mainly IgG3) is the most commonly involved isotype of PGNMID. Here we illustrated a novel medication regimen for the rare variant of PGNMID with deposition of monoclonal immunoglobulin light chain only (PGNMID-LC). Daratumumab has been proved effective in the treatment of plasma cell myeloma while its effect for PGNMID-LC has rarely been reported.

Methods

A daratumumab combination therapy (D-VCd regimen, specifically are daratumumab + dexamethasone + bortezomib + cyclophosphamide) was adopted to treat a patient diagnosed with PGNMID-LC.

Results

The utility of D-VCd regimen showed a favorable effect in this patient. After the fixed course, his clinical symptom, laboratory parameters, neoplastic plasma cells clonity all restored to normal range, and no obvious disease progression was observed throughout the treatment. After a follow up of 14 months, no significant renal or hematological disease progression has been observed.

Conclusion

This case underscores the utility of D-VCd regimen in treatment of PGNMID-LC, and it’s inferred that daratumumab regimen has clinical effects in the disease primarily through targeting tumor clonity. However, data on the use of daratumumab (either in monotherapy or in combination) in clinical trials of PGNMID-LC is currently so limited that that more experiments are needed to support the inference.
Incidental findings on MRI for the evaluation of endometriosis: prevalence and clinical significance
Authors: Harth Sebastian , Roller Fritz Christian , Brose Alexander , Kaya Hasan Emin , Zeppernick Felix , Meinhold-Heerlein Ivo , Krombach Gabriele Anja
Published: October 2024

Objectives

This study aimed to analyze the prevalence and clinical significance of incidental findings on MRI for endometriosis. Differences between patients with and without evidence of deep infiltrating endometriosis on MRI were to be examined.

Methods

This was a retrospective, descriptive cross-sectional single-center study. All patients who received a pelvic MRI for endometriosis between April 2021 and February 2023 were included. The presence and frequency of incidental findings were noted after review of all MR images and radiology reports. The potential clinical significance of the findings was analyzed. Differences in the frequency of incidental findings between patients with and without evidence of deep infiltrating endometriosis on MRI were evaluated, utilizing the Chi-square test, Fisher's exact test and Mann–Whitney U-test.

Results

303 consecutive patients (mean age, 33.4 years ± 8.3) were evaluated. Incidental findings were noted in 299/303 (98.7%) patients. Most frequently, ossification of the hip acetabular rim and degenerative changes of the lumbar spine were noted. In 25/303 (8.3%) patients, incidental findings had high clinical significance. For specific incidental findings, significantly higher prevalences were found in patients with than in patients without evidence of deep infiltrating endometriosis on MRI (hip acetabular rim ossification, p = 0.041; annulus fibrosus fissures, p = 0.006; gallstones, p = 0.042).

Conclusions

Incidental findings are very common on pelvic MRI for endometriosis. The detection of incidental findings can lead to the diagnosis of relevant diseases and thus enable early treatment. On the other hand, many incidental findings have no, only minor, or uncertain consequences.
Advancing healthcare through data: the BETTER project's vision for distributed analytics
Authors: Bregonzio Matteo , Bernasconi Anna , Pinoli Pietro
Published: October 2024

Introduction

Data-driven medicine is essential for enhancing the accessibility and quality of the healthcare system. The availability of data plays a crucial role in achieving this goal.

Methods

We propose implementing a robust data infrastructure of FAIRification and data fusion for clinical, genomic, and imaging data. This will be embedded within the framework of a distributed analytics platform for healthcare data analysis, utilizing the Personal Health Train paradigm.

Results

This infrastructure will ensure the findability, accessibility, interoperability, and reusability of data, metadata, and results among multiple medical centers participating in the BETTER Horizon Europe project. The project focuses on studying rare diseases, such as intellectual disability and inherited retinal dystrophies.

Conclusion

The anticipated impacts will benefit a wide range of healthcare practitioners and potentially influence health policymakers.
Closing the gap: examining the impact of source habitat proximity on plant and soil microbial communities in post-mining spoil heap succession
Authors: Mészárošová Lenka , Kuťáková Eliška , Kohout Petr , Münzbergová Zuzana , Baldrian Petr
Published: October 2024

Introduction

Revegetation of barren substrates is often determined by the composition and distance of the nearest plant community, serving as a source of colonizing propagules. Whether such dispersal effect can be observed during the development of soil microbial communities, is not clear. In this study, we aimed to elucidate which factors structure plant and soil bacterial and fungal communities during primary succession on a limestone quarry spoil heap, focusing on the effect of distance to the adjoining xerophilous grassland.

Methods

We established a grid of 35 plots covering three successional stages – initial barren substrate, early successional community and late successional grassland ecosystem, the latter serving as the primary source of soil colonization. On these plots, we performed vegetation surveys of plant community composition and collected soil cores to analyze soil chemical properties and bacterial and fungal community composition.

Results

The composition of early successional plant community was significantly affected by the proximity of the source late successional community, however, the effect weakened when the distance exceeded 20 m. Early successional microbial communities were structured mainly by the local plant community composition and soil chemical properties, with minimal contribution of the source community proximity.

Discussion

These results show that on small spatial scales, species migration is an important determinant of plant community composition during primary succession while the establishment of soil microbial communities is not limited by dispersal and is primarily driven by local biotic and abiotic conditions.
The evolving hematopoietic niche during development
Authors: Sánchez-Lanzas Raúl , Jiménez-Pompa Amanda , Ganuza Miguel
Published: October 2024

Mammalian hematopoietic stem cells (HSCs) emerge from the hemogenic endothelium in the major embryonic arteries. HSCs undergo a complex journey first migrating to the fetal liver (FL) and from there to the fetal bone marrow (FBM), where they mostly remain during adult life. In this process, a pool of adult HSCs is produced, which sustains lifelong hematopoiesis. Multiple cellular components support HSC maturation and expansion and modulate their response to environmental and developmental cues. While the adult HSC niche has been extensively studied over the last two decades, the niches present in the major embryonic arteries, FL, FBM and perinatal bone marrow (BM) are poorly described. Recent investigations highlight important differences among FL, FBM and adult BM niches and emphasize the important role that inflammation, microbiota and hormonal factors play regulating HSCs and their niches. We provide a review on our current understanding of these important cellular microenvironments across ontogeny. We mainly focused on mice, as the most widely used research model, and, when possible, include relevant insights from other vertebrates including birds, zebrafish, and human. Developing a comprehensive picture on these processes is critical to understand the earliest origins of childhood leukemia and to achieve multiple goals in regenerative medicine, such as mimicking HSC development in vitro to produce HSCs for broad transplantation purposes in leukemia, following chemotherapy, bone marrow failure, and in HSC-based gene therapy.
Modulating oxidative stress and neurogenic inflammation: the role of topiramate in migraine treatment
Authors: Chen Qiao-Wen , Meng Run-Tian , Ko Chih-Yuan
Published: October 2024

Migraine is a chronic, recurrent neurovascular disorder characterized by episodes closely associated with neurovascular hypersensitivity. Oxidative stress can worsen the hypersensitive state of the central nervous system, which in turn can trigger pro-inflammatory factors that result in neurogenic inflammation. Topiramate is frequently used as a preventative measure for migraines, but there is currently little empirical data to support its efficacy through pathways related to neurogenic inflammation and oxidative stress. This review provides an overview of current knowledge regarding the etiology, inducements, pathophysiology, and available treatments for migraine, with a focus on the clinical and experimental evidence of neurogenic inflammation and oxidative stress in migraine. It also delves into the antioxidant and anti-inflammatory qualities of topiramate, clarifying the possible ways in which topiramate affects these pathways to lessen migraine symptoms.
A novel KCNC3 gene variant in the voltage-dependent Kv3.3 channel in an atypical form of SCA13 with dominant central vertigo
Authors: Bernhard Felix P. , Schütte Sven , Heidenblut Moritz , Oehme Moritz , Rinné Susanne , Decher Niels
Published: October 2024

Potassium channel mutations play an important role in neurological diseases, such as spinocerebellar ataxia (SCA). SCA is a heterogeneous autosomal-dominant neurodegenerative disorder with multiple sub-entities, such as SCA13, which is characterized by mutations in the voltage-gated potassium channel Kv3.3 (KCNC3). In this study, we present a rare and atypical case of SCA13 with a predominant episodic central rotational vertigo, while the patient suffered only from mild progressive cerebellar symptoms, such as dysarthria, ataxia of gait and stand, and recently a cognitive impairment. In this patient, we identified a heterozygous variant in KCNC3 (c.2023G > A, p.Glu675Lys) by next-generation sequencing. This Kv3.3E675K variant was studied using voltage-clamp recordings in Xenopus oocytes. While typical SCA13 variants are dominant-negative, show shifts in the voltage-dependence of activation or an altered TBK1 regulation, the Kv3.3E675K variant caused only a reduction in current amplitude and a more pronounced cumulative inactivation. Thus, the differences to phenotypes observed in patients with classical SCA13 mutations may be related to the mechanism of the observed Kv3.3 loss-of-function. Treatment of our patient with riluzole, a drug that is known to also activate potassium channels, turned out to be partly beneficial. Strikingly, we found that the Kv3.3 and Kv3.3E675K inactivation and the frequency-dependent cumulative inactivation was antagonized by increased extracellular potassium levels. Thus, and most importantly, carefully elevated plasma potassium levels in the physiological range, or novel drugs attenuating Kv3.3 inactivation might provide novel therapeutic approaches to rescue potassium currents of SCA13 variants per se. In addition, our findings broaden the phenotypic spectrum of Kv3.3 variants, expanding it to atypical phenotypes of Kv3.3-associated neurological disorders.
Acupuncture treatment of diabetic peripheral neuropathy: an overview of systematic reviews based on evidence mapping
Authors: Jiang Junjie , Shen Hao , Zhang Yi , Li Yuanyuan , Jing Yuanyuan , Chen Xinyi , Wu Hongli , Xie Yanming , Liu Huan
Published: October 2024

Objective

The study attempted to evaluate the meta-analyses (MAs) of the acupuncture treatment of diabetic peripheral neuropathy (DPN) to provide a basis for clinical decision-making.

Methods

Eight databases, such as PubMed, Cochrane Library, Embase, Web of Science, CNKI, Wanfang Data, CQVIP, and CBM, were searched from database creation to December 22, 2023. The MAs of DPN treatment using acupuncture or acupuncture combined with conventional Western medicine were included. AMSTAR-2 and PRISMA 2020 helped evaluate the methodological and reporting quality of the included studies. The GRADE methodology helped assess the evidence quality of outcome indicators. Evidence mapping was performed to display evaluation results.

Results

A total of 18 MAs involving 23,240 DPN patients were included. Based on the methodological quality evaluation, four MAs were of “moderate” quality, seven had a quality grade of “low,” and another seven were of “critically low” quality. The evidence quality evaluation showed that among studies of acupuncture vs. conventional Western medicine, four had an evidence quality of “moderate,” 18 had an evidence quality of “low,” and 17 had an evidence quality of “critically low” and that among studies of acupuncture + conventional Western medicine vs. conventional Western medicine, 12 had an evidence quality of “moderate,” 29 had an evidence quality of “low,” and 33 had an evidence quality of “critically low.” Compared with conventional Western medicine, simple acupuncture and acupuncture + conventional Western medicine significantly improved total effective rate (TER) and nerve conduction velocity (NCV).

Conclusion

Acupuncture treatment of DPN significantly improves TER and NCV with proven safety. However, the MAs of the acupuncture treatment of DPN must strictly refer to relevant standards and specifications regarding methodological and reporting quality, along with the design, execution, and reporting of primary randomized controlled trials (RCTs).
The clinical and neuropsychological profiles of Alzheimer’s disease with white matter hyperintensity in North China
Authors: Chen Yuan , Wang Yan , Zhang Miao , Zhou Yuying , Zhang Huihong , Li Pan , Wu Jialing
Published: October 2024

Background

Patients with Alzheimer’s disease (AD) often exhibit characteristic clinical manifestations, particularly neuropsychiatric symptoms. Previous studies have shown that white matter hyperintensity (WMH) is strongly associated with AD progression, as well as neuropsychiatric symptoms. The purpose of this study was to investigate the clinical and neuropsychological characteristics of AD patients with WMH.

Methods

This retrospective study involved 104 18-fluorodeoxyglucose-positron emission computed tomography (18FDG-PET-CT)-defined AD patients treated at Tianjin Huanhu Hospital from January 2010 to December 2022. Cranial magnetic resonance imaging (MRI) provided semi-quantitative data on brain structure and WMH. Collect and analyze patient clinical data. Neuropsychological assessments were used to evaluate cognitive function and psychobehavioral traits.

Results

Among the 104 patients, 66 were in the WMH group (63.5%) and 38 in the non-white matter hyperintensity (non-WMH) group (36.5%). There were no significant differences in gender, age, age of onset, education, BMI, smoking, drinking, diabetes, coronary heart disease, dementia family history, fasting blood glucose, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) between the two groups. The WMH group showed higher rates of hypertension, homocysteine (Hcy) levels, NPI, and CDR scores as compared to the non-WMH group (p < 0.05). MMSE and MoCA scores were significantly lower in the WMH group (p < 0.05). In the MMSE subitem analysis, patients in the WMH group showed a decrease in attention, recall, and language scores. In the MOCA subitem analysis, WMH patients had lower scores in executive function, naming, attention, language, abstraction, and orientation (p < 0.05). Furthermore, subgroup analysis of NPI showed a higher incidence of delusions, depression, and apathy in the WMH group (p < 0.05). According to the hierarchical analysis of mild, moderate and severe dementia groups, the hypertension, leukoencephalopathy, Hcy level, Fazekas total score, PWMH and DWMH scores in the severe dementia group were significantly higher than those in the mild and moderate dementia groups (p < 0.05). As the disease progresses, more and more patients show increased white matter hyperintensity.

Conclusion

White matter lesions are closely correlated with cognitive decline and psychobehavioral symptoms in AD patients, and may be used as an indicator of disease progression. Priority should be given to early screening and prevention of WMH-related risk factors.
Bibliometric analysis of research trends on factors affecting older adults with mild cognitive impairment
Authors: Yang Lei , Yang Rushi , Wang Bo , Liu Tiantian , Wang Ziyi
Published: October 2024

Background

In recent years, the problem of cognitive impairment in the elderly has become increasingly prominent. Understanding the research trend of influencing factors of mild cognitive impairment, and provide reference for medical staff to early screening of the elderly with mild cognitive impairment.

Objective

Through the visual analysis of the influence factors of the elderly with mild cognitive impairment, the current research status was discussed.

Methods

The relevant literature in the field of influencing factors of mild cognitive impairment in the elderly included in the Web of Science core collection database from 2013 to 2022 was searched. Using software such as Cite Space and VOS viewer to visually analyze literature citations, country, keywords, and development trends.

Results

A total of 547 relevant literatures were included, and the number of publications showed an increasing trend in the past ten years. The United States ranked first in both the number of published papers (157) and centrality (0.34), and the United States and China had a greater influence on the influencing factors of mild cognitive impairment. Alzheimer’s disease, cognitive decline, the elderly, risk factors, are the research hotspot in this field.

Conclusion

Cognitive decline will affect the autonomy of the elderly. Cognitive frailty, MRI is the forefront of MCI research, to understand the research hotspots and frontiers in this field, to conduct early screening and intervention guidance for people with mild cognitive impairment, so as to delay the occurrence of Alzheimer’s disease, and reduce the pressure on family caregivers and society.
Enhanced neural phase locking through audio-tactile stimulation
Authors: Jagt Mels , Ganis Francesco , Serafin Stefania
Published: October 2024

Numerous studies have underscored the close relationship between the auditory and vibrotactile modality. For instance, in the peripheral structures of both modalities, afferent nerve fibers synchronize their activity to the external sensory stimulus, thereby providing a temporal code linked to pitch processing. The Frequency Following Response is a neurological measure that captures this phase locking activity in response to auditory stimuli. In our study, we investigated whether this neural signal is influenced by the simultaneous presentation of a vibrotactile stimulus. Accordingly, our findings revealed a significant increase in phase locking to the fundamental frequency of a speech stimulus, while no such effects were observed at harmonic frequencies. Since phase locking to the fundamental frequency has been associated with pitch perceptual capabilities, our results suggests that audio-tactile stimulation might improve pitch perception in human subjects.
Impact of mini-dose dexmedetomidine supplemented analgesia on sleep structure in patients at high risk of obstructive sleep apnea: a pilot trial
Authors: Sun Pei , Liang Xin-Quan , Chen Na-Ping , Ma Jia-Hui , Zhang Cheng , Shen Yan-E , Zhu Sai-Nan , Wang Dong-Xin
Published: October 2024

Background

Obstructive sleep apnea (OSA) is common in surgical patients and associated with worse perioperative outcomes.

Objectives

To investigate the effect of mini-dose dexmedetomidine supplemented analgesia on postoperative sleep quality pattern in patients at high risk of OSA.

Design

A pilot randomized, double-blind, placebo-controlled trial.

Setting

A tertiary university hospital in Beijing, China.

Patients

One hundred and fifty-two adult patients who had a STOP-Bang score ≥3 and a serum bicarbonate level ≥28 mmol/L and were scheduled for major noncardiac surgery between 29 January 2021 and 20 September 2022.

Intervention

After surgery, patients were provided with high-flow nasal cannula and randomized in a 1:1 ratio to receive self-controlled opioid analgesia supplemented with either mini-dose dexmedetomidine (median 0.02 μg/kg/h) or placebo. We monitored polysomnogram from 9:00 pm to 6:00 am during the first night.

Main outcome measures

Our primary outcome was the percentage of stage 2 non-rapid eye movement (N2) sleep. Secondary and exploratory outcomes included other postoperative sleep structure parameters, sleep-respiratory parameters, and subjective sleep quality (Richards-Campbell Sleep Questionnaire; 0–100 score range, higher score better).

Results

All 152 patients were included in intention-to-treat analysis; 123 patients were included in sleep structure analysis. Mini-dose dexmedetomidine supplemented analgesia increased the percentage of stage N2 sleep (median difference, 10%; 95% CI, 1 to 21%; p = 0.029); it also decreased the percentage of stage N1 sleep (median difference, −10%; 95% CI, −20% to −1%; p = 0.042). Other sleep structure and sleep-respiratory parameters did not differ significantly between the two groups. Subjective sleep quality was slightly improved with dexmedetomidine on the night of surgery, but not statistically significant (median difference, 6; 95% CI, 0 to 13; p = 0.060). Adverse events were similar between groups.

Conclusion

Among patients at high risk of OSA who underwent noncardiac surgery, mini-dose dexmedetomidine supplemented analgesia may improve sleep quality without increasing adverse events.

Clinical trial registration

Clinicaltrials.gov, identifier NCT04608331.
Causal associations between type 2 diabetes mellitus, glycemic traits, dietary habits and the risk of pressure ulcers: univariable, bidirectional and multivariable Mendelian randomization
Authors: Luo Pei , Huang Can
Published: October 2024

Objective

Previous research has established a connection between Type 2 Diabetes Mellitus (T2DM), glycemic traits, dietary habits, and the risk of Pressure Ulcers (PUs). The aim of our study is to disentangle any potential causal relationship between T2DM, glycemic traits, and dietary factors, and the risk of PUs.

Methods

The exposure and outcome datasets were sourced from the IEU Open GWAS project, the Meta-Analyses of Glucose and Insulin-related traits Consortium (MAGIC), and the FinnGen biobank, respectively. The primary MR analysis method employed was the inverse variance-weighted method. Furthermore, we employed multivariable MR (MVMR) adjusting for BMI. Then, we investigated the possibility of a reverse association between glycemic traits and PUs through bidirectional MR. Finally, Heterogeneity and pleiotropic analysis were conducted to ensure the accuracy and robustness of the results.

Results

The findings revealed that T2DM (OR = 1.282, 95% CI: 1.138–1.445, p < 0.001) and Fasting Glucose (FG; OR = 2.111, 95% CI: 1.080–4.129, p = 0.029) were associated with an increased risk of PUs, while salad/raw vegetable intake (OR: 0.014; 95% CI: 0.001–0.278; p = 0.005) was identified as a protective element. However, no other dietary elements demonstrated a statistically significant causality with PUs. In addition, in the reverse direction, there were positive correlation between genetic susceptibility to PUs and an increase in FG (OR: 1.007, 95% CI: 1.000–1.013, p = 0.048) and Fasting Insulin (FI; OR: 1.012, 95% CI: 1.003–1.022, p = 0.011). MVMR results indicated that the causal effect of T2DM on PUs was independent of BMI (OR: 1.260, 95% CI: 1.112–1.427, p < 0.001). These results remained robust when considering weak instrument bias, pleiotropy, and heterogeneity.

Conclusion

This study establishes a causal link between genetically predicted T2DM, FG and an increased risk of PUs. Conversely, Salad/raw vegetable intake is significantly inversely associated with PUs. Simultaneously, we identified two downstream effector factor (FG and FI) that were associated with PUs. These findings may have clinical implications for both prevention and treatment.
Dietary habits, nutritional knowledge, and nutritional status among cardiological patients, including those with obesity and diabetes
Authors: Sapała Anna-Maria , Staśkiewicz-Bartecka Wiktoria , Grochowska-Niedworok Elżbieta , Kardas Marek
Published: October 2024

Background

Cardiovascular disease is a leading cause of death worldwide. The increase in patients with obesity and diabetes raises the risk of cardiovascular diseases. Proper eating habits and adequate nutritional knowledge play a key role in preventing and treating these conditions. This study aimed to evaluate the dietary habits, nutritional knowledge, and nutritional status of patients hospitalized in a cardiology department in Poland, including those with obesity or diabetes.

Methods

The study was conducted at St. Barbara Regional Specialized Hospital No. 5 in Sosnowiec from January to June 2021, involving 301 patients, 154 women (51.2%) and 147 men (48.8%), aged 29 to 87. Participants were assessed for BMI, NRS 2002 scale, morphology, biochemistry results, blood pressure, and examined for nutritional knowledge and habits using proprietary questionnaires. A proprietary scale was used to assess eating habits.

Results

Most cardiology patients were overweight or obese, with 80% exceeding the normal weight range. No significant gender differences were noted in malnutrition risk on the NRS 2002 scale. The study found patients rarely consumed recommended amounts of vegetables, fruits, legumes, whole grains, fish, and dairy products. Only 26.2% regularly ate a second breakfast, and just 9.3% chose water with meals. However, consumption of salty snacks, energy drinks, and alcohol was low. Biochemical and blood test analysis did not show significant differences between patients with diabetes, obesity, and others.

Conclusion

Most cardiology patients were overweight or obese, which poses a significant risk for further health complications, including cardiovascular diseases. Although patients with diabetes and/or obesity had better nutritional knowledge in some areas, this did not lead to healthier eating habits. The absence of significant differences in biochemical tests suggests that overall lifestyle and diet are crucial to cardiovascular health.
Vitamin D deficiency in non-scarring and scarring alopecias: a systematic review and meta-analysis
Authors: Yongpisarn Tanat , Tejapira Kasama , Thadanipon Kunlawat , Suchonwanit Poonkiat
Published: October 2024

Background

Numerous studies have linked vitamin D deficiency (VDD) to the pathogenesis of various alopecia disorders.

Objective

This study aimed to investigate whether patients with alopecia are more likely to have VDD or lower vitamin D levels than controls, and the prevalence of VDD among patients with certain alopecia disorders.

Methods

Electronic searches were conducted using PubMed, Embase, Scopus, and Cochrane Library databases from the dates of their inception until September 2024. Studies that reported data allowing for the calculation of odds ratios, mean differences, or correlation coefficients related to vitamin D levels and alopecia were included, while studies without a confirmed diagnosis of alopecia or those involving patients taking vitamin D supplements were excluded.

Results

It was found that 51.94% of patients with alopecia areata (AA), 50.38% of patients with female pattern hair loss (FPHL), 47.38% of patients with male androgenic alopecia (MAGA), 53.51% of patients with telogen effluvium (TE), and 38.85% of patients with primary scarring alopecia had VDD. Compared to controls, AA patients had a pooled odds ratio (OR) of VDD of 2.84 (95% confidence interval: 1.89–4.26, I2 = 84.29%, p < 0.01) and a pooled unstandardized mean difference (UMD) of vitamin D levels of −8.20 (−10.28 – −6.12, I2 = 74.25%, p < 0.01) ng/mL. For FPHL patients, a pooled OR of VDD of 5.24 (1.50–18.33, I2 = 81.65%, p < 0.01) and a pooled UMD of vitamin D levels of −15.67 (−24.55 – −6.79, I2 = 91.60%, p < 0.01) ng/mL were found. However, for MAGA, a pooled VDD OR of 4.42 (0.53–36.61, I2 = 88.40%, p < 0.01), and a pooled UMD of vitamin D levels of −2.19 ng/mL (−4.07 – −0.31 ng/mL, I2 = 7.64%, p = 0.37) were found. For TE patients, pooled UMD of vitamin D levels of −5.71 (−10.10 – −1.32) ng/mL were found.

Conclusion

People with alopecia frequently have VDD; however, only in patients with AA or FPHL was the association of VDD and decreased vitamin D levels statistically significant compared to control. The findings indicate screening for vitamin D could benefit patients with AA or FPHL, potentially addressing vitamin D deficiency. Further study on vitamin D supplementation as a treatment for alopecia is recommended.
Calculation of alpha particle single-event spectra using a neural network
Authors: Alkhani Layth , Luce Jason P. , Mínguez Gabiña Pablo , Roeske John C.
Published: October 2024

Introduction

A neural network was trained to accurately predict the entire single-event specific energy spectra for use in alpha-particle microdosimetry calculations.

Methods

The network consisted of 4 inputs and 21 outputs and was trained on data calculated using Monte Carlo simulation where input parameters originated both from previously published data as well as randomly generated parameters that fell within a target range. The 4 inputs consisted of the source-target configuration (consisting of both cells in suspension and in tissue-like geometries), alpha particle energy (3.97–8.78 MeV), nuclei radius (2–10 μm), and cell radius (2.5–20 μm). The 21 output values consisted of the maximum specific energy (zmax), and 20 values of the single-event spectra, which were expressed as fractional values of zmax. The neural network consisted of two hidden layers with 10 and 26 nodes, respectively, with the loss function characterized as the mean square error (MSE) between the actual and predicted values for zmax and the spectral outputs.

Results

For the final network, the root mean square error (RMSE) values of zmax for training, validation and testing were 1.57 x10-2, 1.51 x 10-2 and 1.35 x 10-2, respectively. Similarly, the RMSE values of the spectral outputs were 0.201, 0.175 and 0.199, respectively. The correlation coefficient, R2, was > 0.98 between actual and predicted values from the neural network.

Discussion

In summary, the network was able to accurately reproduce alpha-particle single-event spectra for a wide range of source-target geometries.
The value of adjusted PSAD in prostate cancer detection in the Chinese population
Authors: Wang Fangming , Fu Meng , Tang Yuzhe , Li Jianxing
Published: October 2024

Objective

To investigate the value of adjusted prostate-specific antigen density (PSADadj) in the diagnosis of prostate cancer (PCa).

Methods

Data from 410 patients who underwent transrectal ultrasound-guided prostate biopsy were retrospectively analyzed in Beijing Tsinghua Changgung Hospital between November 2014 and March 2024. All patients were divided into PCa and benign prostatic hyperplasia (BPH) groups according to pathological results. Multivariate logistic regression analyses were performed to evaluate the odd ratios (ORs) of predictors for PCa occurrence. Receiver operating characteristic curves were plotted, and the area under the curve (AUC) values were used to assess and compare the diagnostic accuracies of total PSA (tPSA), free-to-total (f/t) PSA, free PSA (fPSA), PSAD, and PSADadj (PSAD×weight).

Results

There were 166 patients in the PCa group and 244 in the BPH group. Multivariate analyses demonstrated that PSAD was positively correlated with the presence of PCa, with the highest OR value among all PSA-related parameters (OR = 19.075, p<0.001). tPSA, fPSAD, PSAD, and PSADadj had high accuracy in predicting PCa, with AUC values of 0.633, 0.730, 0.778, and 0.780. Of note, PSADadj had the highest AUC with a sensitivity of 63.3% and specificity of 81.6%. Similarly, in patients with a PSA level in the gray zone, the diagnostic accuracy of PSADadj in predicting PCa (AUC, 0.709; 95% CI, 0.616–0.802) remained better than other PSA-related markers.

Conclusion

PSADadj has an advantage over other PSA-related markers in detecting PCa and could be used for making biopsy decisions.
Vasopressin induced hyponatremia in infants <3 months of age in the neonatal intensive care unit
Authors: Patel Kavita , Thomson Sharon , Vijayan Meera , Makoni Marjorie , Johnson Peter N. , Stephens Katy , Neely Stephen B. , Miller Jamie L.
Published: October 2024

Objectives

Vasopressin is used for shock and acute pulmonary hypertension in the neonatal intensive care unit (NICU) and is associated with hyponatremia. The purpose of this study was to determine the incidence, severity, contributing risk factors associated with vasopressin-induced hyponatremia in neonates and infants <3 months of age in the NICU. The primary objective was to determine the incidence of hyponatremia (<130 mEq/L) and severe hyponatremia (<125 mEq/L). The secondary objectives were to compare clinical characteristics and the vasopressin regimen between those with and without hyponatremia.

Methods

This retrospective cohort study included neonates and infants <3 months from 1/1/2017–12/31/2022 receiving vasopressin for >6 h. Analyses were performed using SAS v9.4, with a priori less than 0.05. A multiple variable logistic regression was employed to assess odds of hyponatremia.

Results

Of the 105 patients included, 57 (54.3%) developed hyponatremia, and 17 (29.8%) were classified as severe hyponatremia. Overall, the median (interquartile range, IQR) gestational and postnatal age at vasopressin initiation were 35.4 (27–38.7) weeks and 2 (1–12) days. There was no difference in vasopressin dose, but duration of treatment was longer in those with hyponatremia. Higher baseline serum sodium was associated with decreased odds of hyponatremia [adjusted odds ratio (OR): 0.90 (95% CI: 0.83–0.99), p = 0.03], and increased vasopressin duration was associated with increased odds of hyponatremia [aOR: 1.02 (95% CI: 1.01–1.03), p < 0.001].

Conclusions

Hyponatremia occurred in half of patients included. The pre-vasopressin sodium value and the vasopressin duration were independently associated with hyponatremia.
Angiotensin 1 peptide-conjugated CdSe/ZnS quantum dots for cardiac-specific hydrogen sulfide targeted therapy in myocardial ischemia-reperfusion injury
Authors: Wang Qing , Xue Xiaofei , Wang Pei , Yu Yue , Wang Jun , Jiang Qixia , Xiao Jian
Published: October 2024

Introduction

Myocardial ischemia/reperfusion (I/R) injury remains a major obstacle in cardiovascular therapies. Hydrogen sulfide (H2S) shows promise for mitigating I/R injury, but conventional delivery methods, such as NaHS injections or adenovirus-mediated CSE gene therapy, face low efficiency and systemic side effects. This study explores the use of angiotensin 1 (AT1) peptide-conjugated CdSe/ZnS quantum dots (QDs) for targeted delivery of cystathionine-γ-lyase (CSE) plasmids to the myocardium, aiming to boost local H2S production and minimize I/R injury.

Methods

CdSe/ZnS QDs were conjugated with AT1 peptides to create a nanocarrier system capable of delivering the CSE plasmid specifically to the myocardium. In vivo fluorescence imaging confirmed heart-specific accumulation. Myocardial infarct size, cardiac function, cell death, and oxidative stress were evaluated. Endoplasmic reticulum stress and mitophagy markers, including CHOP/GRP78/eIF2α, were analyzed, and the CHOP gene's role was further assessed using an adenovirus vector.

Results

The AT1-conjugated nanocarriers significantly increased CSE expression in the myocardium, as confirmed by fluorescence imaging, without affecting other organs. This localized delivery reduced myocardial infarct size, improved cardiac function, and decreased oxidative stress and cell death. Importantly, a reduction in endoplasmic reticulum stress and mitophagy markers was observed, suggesting that cardioprotection was mediated via the CHOP/GRP78/eIF2α signaling pathway. Reintroduction of CHOP using an adenovirus vector reversed these protective effects, confirming the pathway's involvement.

Discussion

This study demonstrates that AT1 peptide-conjugated QDs can effectively deliver CSE plasmids to the heart, providing significant protection against I/R injury through enhanced localized H2S production. This approach offers a promising, targeted, and side-effect-free therapy for myocardial I/R injury, with potential for clinical translation.
Synergistic effects of peripheral GABA and GABA-transaminase inhibitory drugs on food intake control and weight loss in high-fat diet-induced obese mice
Authors: Nagao Tomoka , Braga Jason D. , Chen Siyi , Thongngam Masubon , Chartkul Maesaya , Yanaka Noriyuki , Kumrungsee Thanutchaporn
Published: October 2024

Background

Developing anti-obesity interventions targeting appetite or food intake, the primary driver of obesity, remains challenging. Here, we demonstrated that dietary γ-aminobutyric acid (GABA) with GABA-degradation inhibitory drugs could be an anti-obesity intervention possessing strong food intake-suppressive and weight-loss effects.

Methods

High-fat (HF)-diet-induced obese mice were divided into six groups receiving either the HF diet or the 2% GABA-HF diet with daily administration of PBS or the GABA-degradation inhibitory drugs, vigabatrin and ethanolamine-O-sulfate (EOS). In 24-h fast-induced refeeding, lean mice with a basal diet were used, and food intake was measured from 0.5 to 24 h after refeeding.

Results

Coadministration of the 2% GABA-HF diet with vigabatrin or EOS significantly decreased food intake (−53%, −35%) and body weight (−22%, −16%) within 11 days in obese mice, along with a marked increase in plasma GABA levels. Mice receiving dietary GABA alone or the drugs alone exhibited no such effects. Hypothalamic GABA levels increased in drug-treated mice, regardless of diet. At 0.5 h after refeeding, food intake was similar in all groups. However, at 0.5 h, plasma GABA levels were markedly increased only in mice receiving coadministration of dietary GABA and the drugs, and their food intake was completely inhibited for over 6 h, while mice in other groups gradually increased their food intake.

Conclusion

Combining dietary GABA with GABA-degradation inhibitory drugs effectively suppresses food intake and promotes weight loss in obese mice, primarily through increased plasma GABA availability. These findings may advance the development of food intake-controlling strategies for obesity management.
Effects of different cold-resistant agents and application methods on yield and cold-resistance of machine-transplanted early rice
Authors: Yuan Shuai , Qin Shiqi , Shi Quan , Chen Pingping , Tu Naimei , Zhou Wenxin , Yi Zhenxie
Published: October 2024

Cold stress is a critical factor affecting rice production worldwide. The application of cold-resistant agents may improve the cold resistance and yield of crops. To screen for suitable cold-resistant agents for machine-transplanted early rice, the effects of uniconazole, abscisic acid, and zinc-amino acids chelate and their spraying times (seed soaking stage, one leaf and one heart stage, two leaves and one heart stage, 7 days before the transplanting stage, and regreening stage) on the yield and cold resistance of machine-transplanted early rice were investigated. Moreover, the application method (spraying amount: 750 and 1125 g ha−1; spraying time: 7 days before the transplanting stage, transplanting stage, regreening stage, and transplanting stage and regreening stage) for the most suitable cold-resistant agent was optimized. The zinc-amino acids chelate was better than the other two cold-resistant agents for promoting rice tillering and increasing the leaf area index, dry matter weight, antioxidant enzyme activities (CAT, SOD, POD) and yield (i.e., 9.22% and 7.14% higher than uniconazole and abscisic acid, respectively), especially when it was applied in the regreening stage. The examination of spraying amounts and times indicated that the zinc-amino acids chelate dosage had no significant effect on the yield and cold resistance of early rice. However, the rice yield and antioxidant enzyme activities were highest when samples were sprayed once in the transplanting stage and the regreening stage. On the basis of the study results, 750 g ha−1 zinc-amino acids chelate applications in the transplanting and regreening stages of machine-transplanted early rice plants may be ideal for increasing cold stress resistance and yield.
Functional characterization of CYP96T1-like cytochrome P450 from Lycoris aurea catalyzing para-para′ and para-ortho′ oxidative coupling in Amaryllidaceae alkaloids biosynthesis
Authors: Liu Zhengtai , Sun Bin , Li Junde , Xiang Yiyu , Wang Rong , Jiang Xiaoran , Zhu Xinning , Xu Sheng , Wang Ren
Published: October 2024

Amaryllidaceae alkaloids (AAs) are complex plant secondary metabolites possessing a wide range of biological activities. 4′-O-methylnorbelladine (4OMN) is the branchpoint intermediate for the entire AAs, and was the last common intermediate before AA pathway branches diverge. The cyclization of 4OMN by C-C oxidative coupling, which can afford para-para′, ortho-para′, and para-ortho′ scaffold, was catalyzed by cytochrome P450 96T (CYP96T) family enzymes. To clarify the mechanisms involved in this controversial step, four CYP96T homologs (LauCYP96T1, LauCYP96T1-like-1, LauCYP96T1-like-2 and LauCYP96T1-like-3) were cloned from the full-length transcriptome of Lycoris aurea. All the four LauCYP96T are localized to endoplasmic reticulum. Functional analysis reveals that LauCYP96T1 and LauCYP96T1-like proteins display inverted regioselectivity for oxidative coupling of 4OMN, in which LauCYP96T1 and LauCYP96T1-like-2 dominantly afford para-para′ scaffold, and LauCYP96T1-like-1 and LauCYP96T1-like-3 are responsible for para-ortho′ scaffold formation. Using molecular homology modeling and docking studies, we predicted models for the binding of 4OMN to LauCYP96T, and identified two amino acid residues that might be responsible for the dominant changes in generated products of para-ortho′ and para-para′ oxidative coupling. Our results highlight the functional diversity and promiscuity of LauCYP96T enzymes and might provide valuable information for Amaryllidaceae alkaloid production.