bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Heat Stress on Growth and Physiological Traits of Alfalfa (Medicago sativa L.) and a Comprehensive Evaluation for Heat Tolerance
Authors: Wassie Misganaw , Zhang Weihong , Zhang Qiang , Ji Kang , Chen Liang
Published: September 2019

Alfalfa (Medicago sativa L.) is a valuable forage legume, but its production is largely affected by high temperature. In this study, we investigated the effect of heat stress on 15 alfalfa cultivars to identify heat-tolerant and -sensitive cultivars. Seedlings were exposed to 38/35 °C day/night temperature for 7 days and various parameters were measured. Heat stress significantly reduced the biomass, relative water content (RWC), chlorophyll content, and increased the electrolyte leakage (EL) and malondialdehyde (MDA) content of heat-sensitive alfalfa cultivars. However, heat-tolerant cultivars showed higher soluble sugar (SS) and soluble protein (SP) content. The heat tolerance of each cultivar was comprehensively evaluated based on membership function value. Cultivars with higher mean membership function value of 0.86 (Bara310SC) and 0.80 (Magna995) were heat tolerant, and Gibraltar and WL712 with lower membership function value (0.24) were heat sensitive. The heat tolerance of the above four cultivars were further evaluated by chlorophyll a fluorescence analysis. Heat stress significantly affected the photosynthetic activity of heat-sensitive cultivars. The overall results indicate that Bara310SC and WL712 are heat-tolerant and heat-sensitive cultivars, respectively. This study provides basic information for understanding the effect of heat stress on growth and productivity of alfalfa.
Postharvest Preservation of the New Hybrid Seedless Grape, ‘BRS Isis’, Grown Under the Double-Cropping a Year System in a Subtropical Area
Authors: Ahmed Saeed , Ruffo Roberto Sergio , Youssef Khamis , Carlos Colombo Ronan , Shahab Muhammad , José Chaves Junior Osmar , Hideki Sumida Ciro , Teodoro de Souza Reginaldo
Published: October 2019

‘BRS Isis’ is a new hybrid seedless table grape tolerant to downy mildew with a good adaptation to the tropical and subtropical climates. Gray mold, caused by Botrytis cinerea Pers. ex Fr. is known as the most important postharvest mold in table grapes, causing extensive losses worldwide. As the postharvest behavior of ‘BRS Isis’ is still unknown, the objective of this work was to evaluate the postharvest preservation and B. cinerea mold control of this new grape cultivar, grown under the double-cropping a year system. Grape bunches were purchased from a field of ‘BRS Isis’ seedless table grapes trained on overhead trellises located at Marialva, state of Parana (South Brazil). Grapes were subjected to the following treatments in a cold room at 1 ± 1 °C: (i) Control; (ii) SO2-generating pad; (iii) control with bunches inoculated with the pathogen suspension; (iv) SO2-generating pad with bunches inoculated with the pathogen suspension. The completely randomized experimental design was used with four treatments, each including five replicates. The incidence of gray mold and other physicochemical variables, including bunch mass loss, shattered berries, skin color index, soluble solids (SS), titratable acidity (TA), and SS/TA ratio of grapes, were evaluated at 50 days after the beginning of cold storage and at seven days at room temperature (22 ± 2 °C). The ‘BRS Isis’ seedless grape, packaged with SO2-generating pads and plastic liners, has a high potential to be preserved for long periods under cold storage, at least for 50 days, keeping very low natural incidence of gray mold, mass loss, and shattered berries.
Expression of Myoepithelial Markers in Mammary Carcinomas of 119 Pet Rabbits
Authors: Degner Sophie , Schoon Heinz-Adolf , Degner Sebastian , Baudis Mathias , Schandelmaier Claudia , Aupperle-Lellbach Heike , Schöniger Sandra
Published: September 2019

Most mammary tumors in pet rabbits are carcinomas; prognostic factors are unknown. The aim of this study on rabbit mammary carcinomas was to determine the expression of myoepithelial markers that have a prognostic relevance in human cancers. Mammary carcinomas (n = 119) of female or female-spayed pet rabbits were immunostained for cytokeratin AE1/AE3, vimentin, smooth muscle actin (SMA), and calponin; and percentages of non-neoplastic myoepithelial cells (ME cells) and calponin-positive neoplastic cells were determined. Using statistical analysis, data were correlated with the age of the rabbits and histological tumor characteristics. All carcinomas contained retained spindle-shaped ME, while 115 also contained hypertrophic ME (HME). A statistically significant relationship existed between a higher age and an increase in HME. In 111 carcinomas (93%), tumor cells expressed calponin. There was a significant correlation between higher percentages of calponin-positive tumor cells and a lower mitotic count, an increased percentage of tubular growth, and a lower grading score, respectively. Data suggest that pet rabbit mammary carcinomas develop from progression of in situ cancer and that the extent of calponin expression in tumor cells influences their biological behavior. These results provide the basis for a long-term follow-up on the prognostic significance of calponin expression in mammary cancer cells.
Thermotolerance of Broiler Chicks Ingesting Dietary Betaine and/or Creatine
Authors: Al-Tamimi Hosam , Mahmoud Kamel , Al-Dawood Amani , Nusairat Basheer , Bani Khalaf Hussam
Published: September 2019

The present study aimed to assess the effect of dietary betaine (B) and/or creatine (C) on performance and thermoregulatory responses of broiler chicks. Indian River broiler chicks, fitted with compact thermosensors, were reared to market age (five weeks). The chicks were randomly distributed into four treatment groups, in a 2 × 2 factorial arrangement of treatments—basal control diet (Control group: CONT; B−/C−); 1 g betaine/kg feed (Betaine group: BETA; B+/C−), 1.2 g creatine monohydrate/kg feed (Creatine group: CRET; B−/C+), and combination (Betaine and Creatine group: COMB; B+/C+) of both supplements. At 31 days of age, 20 chicks from each group were exposed to acute heat stress (A-HS) for 3 h (34.45 ± 0.20 °C), and hemogramic profiles were screened before and after. Performance parameters (feed intake, body weight gain, and feed conversion ratio) were reported on a weekly basis, and carcass meat quality was evaluated at the end of experiment. Redness of breast was higher due to B and C treatments separately than the CONT group (B by C interaction; p < 0.05). Compared to the CONT, dietary supplements alleviated hyperthermia responses, with B alone being more efficient than C or COMB treatments. The mitigation of hyperthermia is likely mediated by enhancement of water balance indicators. Although not efficient in improving growth performance, dietary B and/or C are efficient in improving thermophysiological performance and survival of finishing broiler chicks under A-HS.
Combining Fixed-Time Insemination and Improved Catheter Design in an Effort to Improve Swine Reproduction Efficiency
Authors: McBride Matthew , Amezcua Rocio , Cassar Glen , O’Sullivan Terri , Friendship Robert
Published: September 2019

Conventional practice is to breed sows by artificial insemination (AI) at least twice using approximately three billion sperm per insemination upon estrus at standing heat. This research explored the use of combined technologies, including fixed-time insemination (FTAI) and an alternative catheter design that reportedly reduces semen backflow, in order to reduce the number of inseminations and the semen dosage and maintain reproductive efficiency. The FTAI technique used in this study was to inject I.M. 600 IU equine chorionic gonadotropin (eCG) at weaning and 5 mg porcine luteinizing hormone (pLH) to stimulate ovulation 80 h later, followed by a single insemination 36 h after the pLH injection. The two catheters used in this study were a conventional foam-tipped insemination catheter and a Gedis catheter. The Gedis catheter is designed to be completely inserted into the vagina. The semen is enclosed along the length of the rod and held in place by a gel cap that melts when inserted into the cervix. Sows were assigned to the following treatments: Group 1 (n = 135), bred twice with a conventional catheter and a standard semen dose of approximately three billion sperm in 80 mL; Group 2 (n = 123), FTAI with conventional catheter and a standard semen dose; Group 3 (n = 127), FTAI with Gedis catheter and a standard semen dose; Group 4 (n = 126), FTAI with Gedis catheter and a reduced semen dose with one billion sperm. The farrowing rates were 81.6%, 77.7%, 74.0%, and 62.7% for Groups 1 to 4, respectively. The likelihood of farrowing was lower for Group 3 and Group 4 compared to Group 1 (odds ratio (OR) = 0.57; p = 0.08 and OR = 0.35; p = 0.001, respectively). Likewise, litter size of Group 3 and Group 4 was smaller than Group 1 (p = 0.006 and p = 0.04, respectively). Overall, the combination of Gedis catheter and FTAI resulted in decreased reproductive performance that outweighed the value of using less semen.
Measuring Volumetric Changes of Equine Distal Limbs: A Pilot Study Examining Jumping Exercise
Authors: Johnson Steven , Symons Jennifer
Published: September 2019

Equine athletes can incur musculoskeletal injuries due to repetitive loading during training and competition. Prior to signs of lameness, horse trainers and veterinarians may observe swelling in the distal limbs, where injuries most frequently occur. Early observations may guide modulation of training to manage physiological stress and mitigate risk of injury. However, these observations of changing limb volume can be subjective and imprecise. The aim of this study was to assess the accuracy and applicability of a tablet-mounted, 3D scanner to measure and record distal limb volumes of horses before and after exercise. Users recorded scans of a cylinder of known volume with errors up to 8%. Experienced users’ measures were biased (i.e., consistently overestimated). The scanner was able to detect statistically significant increases in volume for both fore and hind limbs after one jumping session (310–2058 cm3). Age and intensity of workload may play a role in magnitude of limb swelling, but had mixed conclusions between fore and hind limbs. More studies with additional horses must be performed to solidify these relationships. The evaluated 3D scanner is a low-cost, accessible tool that was able to detect changes in limb swelling as a result of exercise and mechanical stress. With continued research, this information may guide training programs to decrease injury and maximize performance of equine athletes in the future.
Proteomic and Membrane Lipid Correlates of Re-duced Host Defense Peptide Susceptibility in a snoD Mutant of Staphylococcus aureus
Authors: Kohler Christian , Proctor Richard , Bayer Arnold , Yeaman Michael , Lalk Michael , Engelmann Susanne , Mishra Nagendra
Published: September 2019

We previously described a transposon mutant in Staphylococcus aureus strain SH1000 that exhibited reduced susceptibility to cationic thrombin-induced platelet microbicidal proteins (tPMPs). The transposon insertion site was mapped to the gene snoD, the staphylococcal nuo orthologue. Hence, further studies have been performed to understand how this mutation impacts susceptibility to tPMP, by comparing proteomics profiling and membrane lipid analyses of the parent vs. mutant strains. Surprisingly, the mutant showed differential regulation of only a single protein when cultivated aerobically (FadB), and only a small number of proteins under anaerobic growth conditions (AdhE, DapE, Ddh, Ald1, IlvA1, AgrA, Rot, SA2366, and SA2367). Corresponding to FadB impact on lipid remodeling, membrane fatty acid analyses showed that the snoD mutant contained more short chain anteiso-, but fewer short chain iso-branched chain fatty acids under both aerobic and anaerobic conditions vs. the parental strain. Based upon these proteomic and membrane compositional data, a hypothetical “network” model was developed to explain the impact of the snoD mutation upon tPMP susceptibility.
Microwave-Assisted Extraction of Ricinine from Ricinus communis Leaves
Authors: Nebo Liliane , Varela Rosa M. , Fernandes João B. , Palma Miguel
Published: October 2019

The alkaloid ricinine (3-cyano-4-methoxy-N-methyl-2-pyridone) is found in different parts of the Ricinus communis plant and is known to possess several bioactive properties, including strong antioxidant activity. In this study, a new microwave-assisted extraction (MAE) method was developed for the recovery of ricinine from R. communis leaves. The extraction variables studied were extraction temperature (between 125 °C and 175 °C), microwave power (between 500 W and 1000 W), extraction time (between 5 min and 15 min), extraction solvent (between 10% and 90% of EtOAc in MeOH), and solvent-to-sample ratio (between 25:1 mL and 50:1 mL of solvent per gram of the sample). On studying the effects of extraction variables, both solvent and liquid-to-solid ratio were found to exhibit the highest effects on ricinine recovery. A fast (15 min) microwave-assisted extraction method was developed (high temperatures can be applied because the stability of ricinine is proven in the literature), allowing for the recovery of ricinine from R. communis leaves. The study revealed that R. communis leaves had almost 1.5 mg g−1 (dried weight) of ricinine.
Glycerolic Licorice Extracts as Active Cosmeceutical Ingredients: Extraction Optimization, Chemical Characterization, and Biological Activity
Authors: Ciganović Petar , Jakimiuk Katarzyna , Tomczyk Michał , Zovko Končić Marijana
Published: October 2019

A green ultrasound-assisted extraction (UAE) method using glycerol/water mixtures for extraction of licorice (Glycyrrhiza glabra) bioactive constituents was developed in this study. The response surface method, according to the Box-Behnken design, was employed to optimize the extraction parameters: glycerol concentration (X1), temperature (X2), and the amount of herbal drug used in the production (X3). The responses were content of total phenols (TP), TP extraction efficiency (TPy) and the content of licorice characteristic constituents, glabridin (Gla) and isoliquiritigenin (Iso). Response surface analysis predicted the optimal extraction conditions for maximized amounts of TP, Tpy, Gla, and Iso. The extracts were prepared using the calculated conditions. The analysis of the selected constituents confirmed the validity of the model. Furthermore, biological activity of the extracts was tested. The results demonstrate that UAE using glycerol is a fast and efficient method for preparation of extracts with excellent radical scavenging, Fe2+ chelating and antioxidant activity. Furthermore, the observed notable tyrosinase and elastase inhibitory activity of the extracts, as well as their anti-inflammatory activity, indicate the anti-aging properties of the investigated extracts. The fact that the extracts were prepared using the safe, cosmetically active solvent, glycerol, makes them suitable for direct use in specialized cosmeceutical formulations.
Probabilistic Characteristics of Moment Capacity and Rotational Stiffness of Wedge Joints Used in Support Systems Reflecting Reused Members
Authors: Bong Jin Kyun , Lee Hyung Do , Kim Seungjun , Mha Ho-Seong , Yim Dong-Kyun , Won Jeong-Hun
Published: September 2019

The moment capacity and rotational stiffness of wedge joints, which connect vertical and horizontal members of assembled support systems, were evaluated experimentally considering the characteristics of reused members. Since temporary structures, such as supports, tend to be reused, experiments were conducted with reused members, and the normality of the measured data was assessed. The lower and upper limits of the 95% confidence intervals of the moment capacity and rotational stiffness of wedge joints with reused members were determined. Experiments were also conducted on a joint system with new members to analyze the influence of reused members. In integrating both new and reused members, the maximum moments of wedge joints were observed to be normally distributed. The lower limit of the 95% confidence interval of the maximum moment of joints was 0.997 kNm, and the upper limit was 1.074 kNm. The rotational stiffness of the wedge joint was evaluated using a trilinear model. The initial rotational stiffness decreased with continued use of the joint. The average rotational stiffness of the joint, analyzed by combining the results for new and reused members, was found to be 22.475 kNm/rad for the first interval, 4.705 kNm/rad for the second interval, and 1.577 kNm/rad for the third interval. The lower limit of the 95% confidence interval of the initial rotational stiffness was 20.688 kNm/rad, and the upper limit was 24.262 kNm/rad.
Direction for High-Performance Supercritical CO2 Centrifugal Compressor Design for Dry Cooled Supercritical CO2 Brayton Cycle
Authors: Cho Seong Kuk , Bae Seong Jun , Jeong Yongju , Lee Jekyoung , Lee Jeong Ik
Published: September 2019

To overcome the degradation of the cycle efficiency of a supercritical carbon dioxide (S-CO2) Brayton cycle with dry cooling, this study proposes an improved design of an S-CO2 centrifugal compressor. The conventional air centrifugal compressor can achieve higher efficiency as backsweep angle increases. However, the structural issue restricts the maximum allowable angle (−50~−56°). In this study, an S-CO2 centrifugal compressor performance was examined while changing the backward sweep angle at impeller exit to study if the previous optimum backsweep angle for an air centrifugal compressor is still valid when the fluid has changed. It is shown through an analysis that an S-CO2 centrifugal compressor can achieve the highest efficiency at −70° backsweep angle, which is greater than the typical design value. The S-CO2 centrifugal compressor is less restricted from a structural integrity issue because it has low relative Mach number regardless of the low sound speed near critical point (Tc = 304.11 K, Pc = 7377 kPa). It is also shown in the paper that the variation of compressibility factor does not impact on its total to total efficiency since its Mach number is still lower than unity. Finally, it is also shown that a backward sweep impeller can achieve higher pressure ratio and operate stably in wider range as the mass flow rate is decreased. As further works, the suggested concept will be validated by the structural analysis and the compressor performance test.
Towards a Universal Semantic Dictionary
Authors: Castro-Bleda Maria Jose , Iklódi Eszter , Recski Gábor , Borbély Gábor
Published: September 2019

A novel method for finding linear mappings among word embeddings for several languages, taking as pivot a shared, multilingual embedding space, is proposed in this paper. Previous approaches learned translation matrices between two specific languages, while this method learns translation matrices between a given language and a shared, multilingual space. The system was first trained on bilingual, and later on multilingual corpora as well. In the first case, two different training data were applied: Dinu’s English–Italian benchmark data, and English–Italian translation pairs extracted from the PanLex database. In the second case, only the PanLex database was used. The system performs on English–Italian languages with the best setting significantly better than the baseline system given by Mikolov, and it provides a comparable performance with more sophisticated systems. Exploiting the richness of the PanLex database, the proposed method makes it possible to learn linear mappings among an arbitrary number of languages.
Antidiabetic Activity of Cactus Acid Fruit Extracts: Simulated Intestinal Conditions of the Inhibitory Effects on α-amylase and α-glucosidase
Authors: Medina-Pérez Gabriela , Zaldívar-Ortega Ana Karen , Cenobio-Galindo Antonio de Jesús , Afanador-Barajas Laura Natali , Vieyra-Alberto Rodolfo , Estefes-Duarte José Antonio , Campos-Montiel Rafael G.
Published: September 2019

Acid cactus fruits “xoconostle” have been used since the pre-Columbian period as a treatment against diverse diseases. In this study, bioactive compounds (phenols and flavonoids) and the in vitro inhibition effect against α-amylase and α-glucosidase were evaluated. Four different extracts of cactus acid fruits were prepared from (1) endocarp, (2) mesocarp, (3) pericarp and (4) whole fruit (WFE). The results showed significant differences (p < 0.05) between extracts. Pericarp extracts had 2.23 ± 0.01 mg of gallic acid equivalents per gram GAE/g of phenol content and 0.84 ± 0.14 mg quercetin equivalents per gram QE/g flavonoid content, while WFE presented 1.52 ± 0.04 mg GAE/g and 0.84 ± 0.14 mg QE/g; however, the inhibition of α-amylase and α-glucosidase were higher with WFE. It was found by using 25 mg/mL of WFE an α-amylase inhibition of 63. ± 1.53% and with 30 mg/mL of WFE an α-glucosidase inhibition of 46.5 ± 1.45% after simulated intestinal conditions. The WFE could be used as a therapeutic strategy in controlled diets of diabetic patients due to its low cost, natural origin, and effect after simulated intestinal conditions.
Validation of Acoustic Emission Waveform Entropy as a Damage Identification Feature
Authors: Santo Farhan Tanvir , Sattar Tariq Pervez , Edwards Graham
Published: September 2019

The increase in the market for supersized LNG (liquefied natural gas) vessels, with double wall cargo tanks, has led to concerns regarding their safe operation. If both the primary and secondary wall of the cargo tank fail simultaneously, the hull of the vessel can be exposed to the LNG. This has the potential to cause brittle failure of the hull structure. This study presents a new acoustic emission (AE) technique approach that can be implemented for monitoring the structural condition of the cargo containment. The new technique approach is based on a feature of the AE waveform, calculated using quadratic Renyi’s entropy. The presented technique is capable of providing information regarding critical damage so that appropriate maintenance can be carried out to avoid failure. The new AE technique is based on an AE feature that is independent of acquisition settings (e.g., threshold and timing), unlike many traditional AE features. The effectiveness of the proposed feature was evaluated by comparison with traditional AE features under ideal conditions for a range of varying acquisition settings. Unlike the traditional feature, the new feature demonstrated no variance with variation of the acquisition settings and was effective in capturing the collective information in the waveform. The proposed AE feature was validated through tensile and fatigue testing on standard specimens of austenitic stainless steel (material of the primary wall). The results suggest that the proposed AE feature is sensitive in identifying the critical damages irrespective of some data acquisition settings.
Emotion-Driven System for Data Center Management
Authors: Corredera Alberto , Romero Marta , Moya Jose M.
Published: September 2019

Complex Information Systems and infrastructures, like Smart Cities, must be efficiently operated, minimizing inefficiencies and maximizing productivity. Traditional approaches are focused on improving the systems, automating processes and services, leaving aside human and emotions considerations. To achieve this efficient operation, we attempted to cover both sides. We found new ways to capture the information coming from the workforce, in our case, the operations management team, and, merged this information with the data from the IoT sensors from the systems, enabling a holistic view of the entire operations occurring in real-time. In a Data Center environment, we have developed a set of tools for capturing the emotional data in order to detect potential biases caused by the specific mood of the person inside the operations team. We used Artificial Intelligence algorithms for finding the patterns that will help us to manage the system in the future. We compared and verified our findings with the existing references from other disciplines, e.g., Psychology. In this article, we expose some methods to be developed in future studies for supervising and increasing productivity in Data Centers, as a useful example for Smart Cities. Our research focuses on monitoring the mood and the emotional status of the personnel responsible for operating the system. We use this emotional data as an input for measurement.
Green Synthesis of a Cu/SiO2 Catalyst for Efficient H2-SCR of NO
Authors: Gioria Esteban , Marchesini F. , Soldati Analía , Giorello Antonella , Hueso Jose , Gutierrez Laura
Published: September 2019

In this work, the synthesis of Cu/SiO2 catalysts starting from pre-formed copper nanoparticle (CuNP) colloidal suspensions was carried out. Two different protocols for the CuNP synthesis were tested: (i) a green approach using water as solvent and ascorbic acid as reducer and stabilizing agent, and (ii) a second solvothermal method involving the use of diethylene glycol as solvent, sodium hypophosphite (NaH2PO2) as reducer, and polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) as stabilizing agents. In addition, and for the sake of comparison, a third catalyst was prepared by solid state conventional grinding of CuO with SiO2. The catalysts were tested in the environmentally relevant catalytic reduction of NOX with H2, in a temperature range from 300 to 500 °C. The catalysts were characterized by X-ray diffraction (XRD), temperature programmed reduction (TPR) cycles, Raman spectroscopy, and N2 adsorption for specific surface BET measurements. From these techniques CuO and Cu(0) species were detected depending on the synthesis protocol. CuNP size and size distribution in the colloid suspensions were determined by transmission electronic microscopy (TEM). The catalyst prepared from the aqueous suspension (CuAsc/SiO2) exhibited higher NO conversion (100%) and selectivity (85%) toward N2 at the lower reaction evaluated temperature (300 °C). The CuCTAB/SiO2 catalyst obtained by the solvothermal approach showed activity at high reaction temperature (400 °C) preferentially. The metal–support mechanical mixture exhibited a negligible response at low temperature and low conversion (68%) and selectivity (88%) at 500 °C. Nanoparticle size and distribution on the support, together with the metal–support interaction, were postulated as the most plausible parameters governing the catalytic performance of the different Cu/SiO2 materials.
Thermal Evaluation of a Hybrid Dryer with Solar and Geothermal Energy for Agroindustry Application
Authors: Delgado-Plaza Emérita , Peralta-Jaramillo Juan , Quilambaqui Miguel , Gonzalez Olga , Reinoso-Tigre José , Arevalo Anthony , Arancibia Mirari , Paucar Mayra , Velázquez-Martí Borja
Published: September 2019

The process of drying at the agroindustry level is considered the most important stage for the storage and conservation of food, but an inadequate sizing of the systems can generate an excessive consumption of fuel and energy. In the case of the agricultural sector, especially in small and medium producers in Ecuador, they tend to dry cereals outdoors, spreading the product over black plastics, asphalt floors, or cements called “tendal”. This process depends on the weather conditions of the area and can take between one or four days of drying, tending to damage the product by fungi. On the other hand, certain producers build dryers empirically, using as the main fuel gas for domestic use, diesel, or firewood. Among the problems presented for these types of dryers are heat losses in the drying chamber as it is not thermally insolated, a lack of temperature control (above 60 °C) and drying speed, excessive fuel use, and 10% burned product, which are essential to control to obtain a quality product and savings in the energy consumption of the dryer. With regard to the problem raised above, the design and construction of a hybrid dryer that worked with 80% of thermal solar and low enthalpy aerogeothermal energy and 20% with electric power was conducted to optimize the drying process and ensure a quality dry product. The work proposes the dimensioning of each component of the dryer. As a result, it was found that the efficiency of the equipment was around 60%, reaching a maximum operating temperature of 52 °C, with a stabilization time of the system in the first hour within 40 min. It should be noted that the design of the prototype is easy to adapt and replicate.
New Insights from Multibody Dynamic Analyses of a Turnout System under Impact Loads
Authors: Hamarat Mehmet , Kaewunruen Sakdirat , Papaelias Mayorkinos , Silvast Mika
Published: September 2019

A railway turnout is an essential infrastructure for managing railway traffic flexibility. In contrast, it imposes restrictions on train operations such as lower operational speeds through the turnout due to the complex movements of trains over the turnout. This results in the large-amplitude dynamic responses of the train-turnout interaction. Previous studies have focused on the train-turnout interactions entailing the wheel-rail contact forces and stresses. Very few of the studies considered the effects of the contact forces on the turnout structure and its components such as sleepers and bearers. Those previous studies neglected the dynamic forces and estimated the behavior of train-turnout interactions based on quasi-static calculations. In reality, turnouts are subjected to high impact forces, which can be higher than the permissible track forces. Consequently, a numerical model capable of determining impact forces was developed here, to evaluate the dynamic behaviors of a railway turnout and their effects on such turnout components as bearers, ballast, and so on. The model consists of a structured beam grillage laying on an elastic foundation with rigid wheelsets and a bogie. The model was verified by field measurements. The new insight stemmed from this study shows that neglecting the contribution of dynamic forces can result in the unsafe underestimation of train turnout behaviors.
Biomechanical Performance of the Cemented Hip Stem with Different Surface Finish
Authors: Hung Jui-Pin , Bai Yu-Wei , Hung Chung-Qua , Lee Tsui-Er
Published: September 2019

The integrity of the cemented fixation interface is responsible for the long-term longevity of artificial hip prostheses. Metallic stems with roughened surfaces are considered to provide stronger adhesion with cement. However, clinical studies have reported that roughened stems show a lower survival rate than polished stems. These studies clearly reveal that the causes of artificial stem loosening are very complicated and multifaceted. Therefore, this study was conducted to investigate the mechanical effect of stem surface finish in cemented hip replacement. To accomplish this, a series of cement–metal specimens were tested configurations to assess the mechanical characteristics of the cement–metal interface specimens. A finite elemental model of cemented femoral prostheses was then created, in which the cement–stem interface was assumed to be in different bonding states according to the experimentally measured interface properties. The failure probabilities of the cement mantle and cemented interface under physiological loadings were evaluated. Experimental results indicate that the polished metal produced higher interfacial tensile and lower shearing strengths than the roughened metal. The polished stems were predicted to induce a lower failure probability of cement mantle and higher integrity of the cement–stem interface when compared to the roughened stem. Overall, current results provide significant evidence to support the clinical outcomes of cemented hip prostheses with different stem surface finishes.
Tailored Chip Breaker Development for Polycrystalline Diamond Inserts: FEM-Based Design and Validation
Authors: Cascón Itxaso , Sarasua Jon Ander , Elkaseer Ahmed
Published: October 2019

Chip evacuation is a critical issue in metal cutting, especially continuous chips that are generated during the machining of ductile materials. The improper evacuation of these kinds of chips can cause scratching of the machined surface of the workpiece and worsen the resultant surface quality. This scenario can be avoided by using a properly designed chip breaker. Despite their relevance, chip breakers are not in wide-spread use in polycrystalline diamond (PCD) cutting tools. This paper presents a systematic methodology to design chip breakers for PCD turning inserts through finite element modelling. The goal is to evacuate the formed chips from the cutting zone controllably and thus, maintain surface quality. Particularly, different scenarios of the chip formation process and chip curling/evacuation were simulated for different tool designs. Then, the chip breaker was produced by laser ablation. Finally, experimental validation tests were conducted to confirm the ability of this chip breaker to evacuate the chips effectively. The machining results revealed superior performance of the insert with chip breaker in terms of the ability to produce curly chips and high surface quality (Ra = 0.51–0.56 µm) when compared with the insert without chip breaker that produced continuous chips and higher surface roughness (Ra = 0.74–1.61 µm).
Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use
Authors: Lecocq Quentin , Zeven Katty , De Vlaeminck Yannick , Martens Sandrina , Massa Sam , Goyvaerts Cleo , Raes Geert , Keyaerts Marleen , Breckpot Karine , Devoogdt Nick
Published: September 2019

Immune checkpoint inhibition (ICI) is a promising cancer therapy, which has progressed rapidly from a preclinical concept to clinical implementation. Commonly considered targets in ICI are CTLA-4, PD-1/PD-L1, and LAG-3, and the list grows. As ICI is generally only beneficial for a subset of patients, there is a need to select patients that are eligible for therapy as well as to monitor therapy response. There is growing interest to do this noninvasively, by molecular imaging with target-specific tracers. To this day, noninvasive imaging has focused on CTLA-4 and PD-1/PD-L1, while there is no noninvasive tool available to accurately assess LAG-3 expression in vivo. In this proof-of-concept study, we developed nanobodies, the smallest functional fragments from camelid heavy chain-only antibodies, to noninvasively evaluate mouse LAG-3 expression using single positron emission computed tomography (SPECT)/CT imaging. The in vitro characterization of 114 nanobodies led to the selection of nine nanobodies binding to mouse LAG-3. The injection of 99mTechnetium-labeled nanobodies in healthy mice showed specific uptake in immune peripheral organs like the spleen and lymph nodes, which was not observed in LAG-3 gene knock-out mice. Moreover, nanobody uptake could be visualized using SPECT/CT and correlated to the presence of LAG-3 as assessed in flow cytometry and immunohistochemistry. SPECT/CT scans of tumor bearing mice further confirmed the diagnostic potential of the nanobodies. These findings substantiate the approach to use nanobodies as a tool to image inhibitory immune checkpoints in the tumor environment.
Brusatol, a Nrf2 Inhibitor Targets STAT3 Signaling Cascade in Head and Neck Squamous Cell Carcinoma
Authors: Lee Jong Hyun , Rangappa Shobith , Mohan Chakrabhavi Dhananjaya , Basappa  , Sethi Gautam , Lin Zhi-Xiu , Rangappa Kanchugarakoppal S. , Ahn Kwang Seok
Published: September 2019

STAT3 is a latent transcription factor that plays a vital role in the transmission of extracellular signal from receptors to the nucleus. It has been regarded as a master transcription factor due to its role in the regulation of a broad spectrum of genes, which can contribute to oncogenesis. Persistent activation of STAT3 and deregulation of its signaling has been observed in various human cancers including head and neck squamous cell carcinoma (HNSCC). In the present work, we identified brusatol (BT) as a potential blocker of STAT3 signaling pathway in diverse HNSCC cells. The data from the cell-based experiments suggested that BT-induced cytotoxicity and abrogated the activation of STAT3 and that of upstream kinases such as JAK1, JAK2, and Src. It reduced the levels of nuclear STAT3 and its DNA binding ability. BT treatment increased annexin-V-positive cells, promoted procaspase-3 and PARP cleavage, and downregulated the mRNA and protein expression of diverse proteins (Bcl-2, Bcl-xl, survivin) in HNSCC cells. Taken together, brusatol can function as a promising inhibitor targeting STAT3 signaling pathway in HNSCC.
Characterization of the Anti-Cancer Activity of the Probiotic Bacterium Lactobacillus fermentum Using 2D vs. 3D Culture in Colorectal Cancer Cells
Authors: Lee Joo-Eun , Lee Jina , Kim Ji Hyun , Cho Namki , Lee Sung Hoon , Park Sung Bum , Koh Byumseok , Kang Dukjin , Kim Seil , Yoo Hee Min
Published: October 2019

The aim of this study was to investigate the potential anti-cancer effects of probiotic cell-free supernatant (CFS) treatment using Lactobacillus fermentum for colorectal cancer (CRC) in 3D culture systems. Cell viability was assessed using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) assays, whereas apoptosis was monitored through RT-qPCR analysis of Bax, Bak, Noxa, and Bid mRNA expressions in addition to flow cytometry analysis of Lactobacillus cell-free supernatant (LCFS) treatment. Our results showed that the anti-cancer effect of LCFS on cell viability was pronouncedly enhanced in 3D-cultured HCT-116 cells, which was linked to the increased level of cleaved caspase 3. Additionally, upregulation of apoptotic marker gene mRNA transcription was dramatically increased in 3D cultured cells compared to 2D systems. In conclusion, this study suggests that LCFS enhances the activation of intrinsic apoptosis in HCT-116 cells and the potential anti-cancer effects of Lactobacilli mixtures in 3D culture systems. All in all, our study highlights the benefits of 3D culture models over 2D culture modeling in studying the anti-cancer effects of probiotics.
P120 Catenin Isoforms Differentially Associate with Breast Cancer Invasion and Metastasis
Authors: Venhuizen Jan-Hendrik , Span Paul , van den Dries Koen , Sommer Sebastian , Friedl Peter , Zegers Mirjam
Published: September 2019

Tumor metastasis is the endpoint of tumor progression and depends on the ability of tumor cells to locally invade tissue, transit through the bloodstream and ultimately to colonize secondary organs at distant sites. P120 catenin (p120) has been implicated as an important regulator of metastatic dissemination because of its roles in cell–cell junctional stability, cytoskeletal dynamics, growth and survival. However, conflicting roles for p120 in different tumor models and steps of metastasis have been reported, and the understanding of p120 functions is confounded by the differential expression of p120 isoforms, which differ in N-terminal length, tissue localization and, likely, function. Here, we used in silico exon expression analyses, in vitro invasion assays and both RT-PCR and immunofluorescence of human tumors. We show that alternative exon usage favors expression of short isoform p120-3 in 1098 breast tumors and correlates with poor prognosis. P120-3 is upregulated at the invasive front of breast cancer cells migrating as collective groups in vitro. Furthermore, we demonstrate in histological sections of 54 human breast cancer patients that p120-3 expression is maintained throughout the metastatic cascade, whereas p120-1 is differentially expressed and diminished during invasion and in metastases. These data suggest specific regulation and functions of p120-3 in breast cancer invasion and metastasis.
The Role of TRAIL/DRs in the Modulation of Immune Cells and Responses
Authors: Sag Duygu , Ayyildiz Zeynep Ozge , Gunalp Sinem , Wingender Gerhard
Published: September 2019

Expression of TRAIL (tumor necrosis factor–related apoptosis–inducing ligand) by immune cells can lead to the induction of apoptosis in tumor cells. However, it becomes increasingly clear that the interaction of TRAIL and its death receptors (DRs) can also directly impact immune cells and influence immune responses. Here, we review what is known about the role of TRAIL/DRs in immune cells and immune responses in general and in the tumor microenvironment in particular.
The Role of the Cyclin Dependent Kinase Inhibitor p21cip1/waf1 in Targeting Cancer: Molecular Mechanisms and Novel Therapeutics
Authors: Al Bitar Samar , Gali-Muhtasib Hala
Published: September 2019

p21cip1/waf1 mediates various biological activities by sensing and responding to multiple stimuli, via p53-dependent and independent pathways. p21 is known to act as a tumor suppressor mainly by inhibiting cell cycle progression and allowing DNA repair. Significant advances have been made in elucidating the potential role of p21 in promoting tumorigenesis. Here, we discuss the involvement of p21 in multiple signaling pathways, its dual role in cancer, and the importance of understanding its paradoxical functions for effectively designing therapeutic strategies that could selectively inhibit its oncogenic activities, override resistance to therapy and yet preserve its tumor suppressive functions.
Enhanced Visible Light Photodegradation of Microplastic Fragments with Plasmonic Platinum/Zinc Oxide Nanorod Photocatalysts
Authors: Tofa Tajkia Syeed , Ye Fei , Kunjali Karthik Laxman , Dutta Joydeep
Published: September 2019

Microplastics are persistent anthropogenic pollutants which have become a global concern owing to their widespread existence and unfamiliar threats to the environment and living organisms. This study demonstrates the degradation of fragmented microplastics particularly low-density polyethylene (LDPE) film in water, through visible light-induced plasmonic photocatalysts comprising of platinum nanoparticles deposited on zinc oxide (ZnO) nanorods (ZnO-Pt). The ZnO-Pt nanocomposite photocatalysts were observed to have better degradation kinetics for a model organic dye (methylene blue) compared to bare ZnO nanorods, attributed to the plasmonic effects leading to better interfacial exciton separation and improved hydroxyl radical activity along with a 78% increase in visible light absorption. These demonstrations of the plasmonically enhanced photocatalyst enabled it to effectively degrade microplastic fragments as confirmed following the changes in carbonyl and vinyl indices in infrared absorption. In addition, visual proof of physical surface damage of the LDPE film establishes the efficacy of using plasmonically enhanced nanocomposite photocatalytic materials to tackle the microplastic menace using just sunlight for a clean and green approach towards mitigation of microplastics in the ecosystem.
Multi-Catalytic Route for the Synthesis of (S)-Tembamide
Authors: Leemans Laura , Walter Marc D. , Hollmann Frank , Schallmey Anett , van Langen Luuk M.
Published: September 2019

Enantiopure β-amino alcohols constitute one of the most significant building blocks for the synthesis of active pharmaceutical ingredients. Despite the availability of a range of chiral β-amino alcohols from a chiral pool, there is a growing demand for new enantioselective synthetic routes to vicinal amino alcohols and their derivatives. In the present study, an asymmetric 2-step catalytic route that converts 4-anisaldehyde into a β-amino alcohol derivative, (S)-tembamide, with excellent enantiopurity (98% enantiomeric excess) has been developed. The recently published initial step consists in a concurrent biocatalytic cascade for the synthesis of (S)-4-methoxymandelonitrile benzoate. The O-benzoyl cyanohydrin is then converted to (S)-tembamide in a hydrogenation reaction catalyzed by Raney Ni. To achieve hydrogenation of the nitrile moiety with highest chemoselectivity and enantioretention, various parameters such as nature of the catalyst, reaction temperature and hydrogen pressure were studied. The reported strategy might be transferrable to the synthesis of other N-acyl-β-amino alcohols.
Synthesis of GO-SalenMn and Asymmetric Catalytic Olefin Epoxidation
Authors: Wang Fengqin , Huang Tiankui , Rao Shurong , Chen Qian , Huang Cheng , Tan Zhiwen , Ding Xiyue , Zou Xiaochuan
Published: September 2019

Graphene oxide (GO) was used as a catalyst carrier, and after the hydroxyl group in GO was modified by 3-aminopropyltrimethoxysilane (MPTMS), axial coordination and immobilization with homogeneous chiral salenMnCl catalyst were carried out. The immobilized catalysts were characterized in detail by FT–IR, TG–DSC, XPS, EDS, SEM, X-ray, and AAS, and the successful preparation of GO-salenMn was confirmed. Subsequently, the catalytic performance of GO-salenMn for asymmetric epoxidation of α-methyl-styrene, styrene, and indene was examined, and it was observed that GO-salenMn could efficiently catalyze the epoxidation of olefins under an m-CPBA/NMO oxidation system. In addition, α-methyl-styrene was used as a substrate to investigate the recycling performance of GO-salenMn. After repeated use for three times, the catalytic activity and enantioselectivity did not significantly change, and the conversion was still greater than 99%. As the number of cycles increased, the enantioselectivity and chemoselectivity gradually decreased, but even after 10 cycles, the enantiomeric excess was 52%, which was higher than that of the homogeneous counterpart under the same conditions. However, compared to fresh catalysts, the yield decreased from 96.9 to 55.6%.
Hydrolysis of Glycosyl Thioimidates by Glycoside Hydrolase Requires Remote Activation for Efficient Activity
Authors: Guillotin Laure , Assaf Zeinab , Pistorio Salvatore G. , Lafite Pierre , Demchenko Alexei V. , Daniellou Richard
Published: October 2019

Chemoenzymatic synthesis of glycosides relies on efficient glycosyl donor substrates able to react rapidly and efficiently, yet with increased stability towards chemical or enzymatic hydrolysis. In this context, glycosyl thioimidates have previously been used as efficient donors, in the case of hydrolysis or thioglycoligation. In both cases, the release of the thioimidoyl aglycone was remotely activated through a protonation driven by a carboxylic residue in the active site of the corresponding enzymes. A recombinant glucosidase (DtGly) from Dictyoglomus themophilum, previously used in biocatalysis, was also able to use such glycosyl thioimidates as substrates. Yet, enzymatic kinetic values analysis, coupled to mutagenesis and in silico modelling of DtGly/substrate complexes demonstrated that the release of the thioimidoyl moiety during catalysis is only driven by its leaving group ability, without the activation of a remote protonation. In the search of efficient glycosyl donors, glycosyl thioimidates are attractive and efficient. Their utility, however, is limited to enzymes able to promote leaving group release by remote activation.