bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering
Authors: Gębczak Katarzyna , Wiatrak Benita , Fortuna Wojciech
Published: July 2021

The use of extracellular matrix (ECM) biomaterials for soft tissue repair has proved extremely successful in animal models and in some clinical settings. The aim of the study was to investigate the effect of the commercially obtained CorMatrix bioscaffold on the viability, proliferation and migration of rat pheochromocytoma cell line PC12. PC12 cells were plated directly onto a CorMatrix flake or the well surface of a 12-well plate and cultured in RPMI-1640 medium and a medium supplemented with the nerve growth factor (NGF). The surface of the culture plates was modified with collagen type I (Col I). The number of PC12 cells was counted at four time points and then analysed for apoptosis using a staining kit containing annexin V conjugate with fluorescein and propidium iodide (PI). The effect of CorMatrix bioscaffold on the proliferation and migration of PC12 cells was tested by staining the cells with Hoechst 33258 solution for analysis using fluorescence microscopy. The research showed that the percentage of apoptotic and necrotic cells was low (less than 7%). CorMatrix stimulates the proliferation and possibly migration of PC12 cells that populate all levels of the three-dimensional architecture of the biomaterial. Further research on the mechanical and biochemical capabilities of CorMatrix offers prospects for the use of this material in neuro-regenerative applications.
Biodegradable Nanofibrous Membranes for Medical and Personal Protection Applications: Manufacturing, Anti-COVID-19 and Anti-Multidrug Resistant Bacteria Evaluation
Authors: Alshabanah Latifah Abdullah , Hagar Mohamed , Al-Mutabagani Laila A. , Abozaid Ghada M. , Abdallah Salwa M. , Ahmed Hoda , Hassanin Ahmed H. , Shehata Nader
Published: July 2021

Biodegradable nanofibrous hybrid membranes of polyvinyl alcohol (PVA) with ZnO and CuO nanoparticles were manufactured and characterized, and their anti-COVID-19 and anti-multidrug resistant bacteria activities were also evaluated. The morphological structures of the prepared PVA composites nanofibers were observed by scanning electron microscope (SEM), which revealed a homogenous pattern of the developed nanofibers, with an average fibrous diameter of 200–250 nm. Moreover, the results of the SEM showed that the fiber size changed with the type and the concentration of the metal oxide. Moreover, the antiviral and antibacterial potential capabilities of the developed nanofibrous membranes were tested in blocking the viral fusion of SARS-COV-2, as a representative activity for COVID-19 deactivation, as well as for their activity against a variety of bacterial strains, including multi-drug resistant bacteria (MDR). The results revealed that ZnO loaded nanofibers were more potent antiviral agents than their CuO analogues. This antiviral action was attributed to the fact that inorganic metallic compounds have the ability to extract hydrogen bonds with viral proteins, causing viral rupture or morphological changes. On the other hand, the anti-multi-drug resistant activity of the prepared nanofibers was also evaluated using two techniques; the standard test method for determining the antimicrobial activity of immobilized antimicrobial agents under dynamic contact conditions and the standard test method for determining the activity of incorporated antimicrobial agents in polymeric or hydrophobic materials. Both techniques proved the superiority of the ZnO loaded nanofibers over the CuO loaded fibers. The results of the antiviral and antibacterial tests showed the effectiveness of such nanofibrous formulas, not only for medical applications, but also for the production of personal protection equipment, such as gowns and textiles.
Thermal Shock Resistance and Thermal Insulation Capability of Laser-Glazed Functionally Graded Lanthanum Magnesium Hexaluminate/Yttria-Stabilised Zirconia Thermal Barrier Coating
Authors: Anaz Khan Muhammed , Vivek Anand Annakodi , Duraiselvam Muthukannan , Srinivas Rao Koppula , Arvind Singh Ramachandra , Jayalakshmi Subramanian
Published: July 2021

In this work, functionally graded lanthanum magnesium hexaluminate (LaMgAl11O19)/yttria-stabilised zirconia (YSZ) thermal barrier coating (FG-TBC), in as-sprayed and laser-glazed conditions, were investigated for their thermal shock resistance and thermal insulation properties. Results were compared with those of a dual-layered coating of LaMgAl11O19 and YSZ (DC-TBC). Thermal shock tests at 1100 °C revealed that the as-sprayed FG-TBC had improved thermal stability, i.e., higher cycle lifetime than the as-sprayed DC-TBC due to its gradient architecture, which minimised stress concentration across its thickness. In contrast, DC-TBC spalled at the interface due to the difference in the coefficient of thermal expansion between the LaMgAl11O19 and YSZ layers. Laser glazing improved cycle lifetimes of both the types of coatings. Microstructural changes, mainly the formation of segmentation cracks in the laser-glazed surfaces, provided strain tolerance during thermal cycles. Infrared rapid heating of the coatings up to 1000 °C showed that the laser-glazed FG-TBC had better thermal insulation capability, as interlamellar pores entrapped gas and constrained heat transfer across its thickness. From the investigation, it is inferred that (i) FG-TBC has better thermal shock resistance and thermal insulation capability than DC-TBC and (ii) laser glazing can significantly enhance the overall thermal performance of the coatings. Laser-glazed FG-TBC provides the best heat management, and has good potential for applications that require effective heat management, such as in gas turbines.
Life Cycle Assessment of Sustainable Asphalt Pavement Solutions Involving Recycled Aggregates and Polymers
Authors: Oreto Cristina , Russo Francesca , Veropalumbo Rosa , Viscione Nunzio , Biancardo Salvatore Antonio , Dell’Acqua Gianluca
Published: July 2021

The pursuit of sustainability in the field of road asphalt pavements calls for effective decision-making strategies, referring to both the technical and environmental sustainability of the solutions. This study aims to compare the life cycle impacts of several pavement solution alternatives involving, in the binder and base layers, some eco-designed, hot- and cold-produced asphalt mixtures made up of recycled aggregates in substitution for natural filler and commercial recycled polymer pellets for dry mixture modification. The first step focused on the technical and environmental compatibility assessment of the construction and demolition waste (CDW), jet grouting waste (JGW), fly ash (FA), and reclaimed asphalt pavement (RAP). Then, three non-traditional mixtures were designed for the binder layer and three for the base layer and characterized in terms of the stiffness modulus. Asphalt pavement design allowed for the definition of the functional units of Life Cycle Assessment (LCA), which was applied to all of the pavement configurations under analysis in a “from cradle to grave” approach. The LCA results showed that the best performance was reached for the solutions involving a cold, in-place recycled mixture made up of RAP and JGW in the base layer, which lowered all the impact category indicators by 31% on average compared to those of the traditional pavement solution. Further considerations highlighted that the combination of a cold base layer with a hot asphalt mixture made up of CDW or FA in the binder layer also maximized the service life of the pavement solution, providing the best synergistic effect.
Free Cells in Hyperspaces of Graphs
Authors: Morales José Ángel Juárez , Hernández Gerardo Reyna , Valencia Jesús Romero , Cayetano Omar Rosario
Published: July 2021

Often for understanding a structure, other closely related structures with the former are associated. An example of this is the study of hyperspaces. In this paper, we give necessary and sufficient conditions for the existence of finitely-dimensional maximal free cells in the hyperspace C(G) of a dendrite G; then, we give necessary and sufficient conditions so that the aforementioned result can be applied when G is a dendroid. Furthermore, we prove that the arc is the unique arcwise connected, compact, and metric space X for which the anchored hyperspace Cp(X) is an arc for some p∈X.
Teaching Attitudes towards Students with Disabilities
Authors: Gallego-Ortega José Luis , Rodríguez-Fuentes Antonio
Published: July 2021

(1) Background: Inclusive education has been a recurring topic during the last decades. However, not every teacher is equally enthusiastic about how to implement it. Understanding these discrepancies can help to identify improvement procedures. Therefore, even though teachers’ beliefs and attitudes around inclusion have been thoroughly explored, it is necessary to delve further on this type of study to understand possible changes derived from the socio-educational transformations experimented by current societies. (2) Methods: This study examined the attitudes of 122 teachers, both Primary and Special Needs ones, working in rural and urban schools, regarding inclusion of students with disabilities. The data were collected from two scales: Attitude Survey Inclusive Education–Teachers (ASIE-T) and Scale for Measuring Pre-Service Teachers’ Perceptions about Inclusion the Sentiments, Attitudes and Concerns about Inclusive Education Revised (SACIE-R). A quantitative approach was employed, of descriptive and correlational type. Descriptive and inferential analyses were performed by means of parametric tests. Furthermore, the bivariate correlation technique Person’s r was carried out in order to verify the intensity among variables. The effect sizes are provided as Cohen’s d. (3) Results: The results revealed positive beliefs concerning inclusion and the existence of differences among teachers, depending on their specialisation and gender. The variables “age” and “stage of education” were not significant in terms of generating in terms of attitude generation. (4) Conclusions: Understanding the association between socio-demographic variables and stigma is crucial when it comes to accomplishing an inclusive and high-quality education. The general findings of this study contribute to justify the implementation of programmes to stimulate and encourage meaningful interactions between general education teachers and students with disabilities.
Induction Brazing for Rapid Localized Repair of Inconel 718
Authors: Aprilia Aprilia , Tan Jin Lee , Yang Yongjing , Tan Sung Chyn , Zhou Wei
Published: July 2021

Vacuum furnace has been used for brazing repair of aerospace components, but it is a slow process which typically takes a few hours. The prolonged heating and cooling cycles could cause some adverse effects on the components such as excessive grain growth. A rapid brazing technique using induction coil was studied to evaluate its suitability for localized repair. Induction brazing of Inconel 718 was carried out using AMS 4777 brazing paste at different temperatures (950 °C, 1050 °C and 1150 °C ) for various durations (2 min, 10 min and 20 min). Microstructure and microhardness were evaluated. The experimental results show that brazing at 1050 °C leads to desirable microstructures in a short period of merely 2 min. The study demonstrates the potential application of induction brazing for rapid localized aerospace repair.
Thermophysical Properties of Pipe Steel in the Liquid State
Authors: Tsepelev Vladimir S. , Starodubtsev Yuri N. , Tsepeleva Nadezhda P.
Published: July 2021

The temperature dependences of the kinematic viscosity and surface tension of liquid pipe steel with different modes of melt preparation were investigated. A transition zone was found on the temperature dependences of the thermophysical properties, which separates the regions with different activation energies of viscous flow and surface tension. At the heating stage in the transition zone, the thermal decomposition of clusters based on cementite Fe3C occurs. As a result of the decomposition, free carbon atoms appear which tend to give a uniform distribution in liquid iron with increasing temperature. At a low content of alloying elements and impurities, a high-temperature melt should have a large-scale cluster structure, which provides a more uniform distribution of chemical elements. The melt after vacuum degassing has a narrow transition zone near 1920 K, in contrast to the wide transition zone of the melt without vacuum degassing. The wider transition zone is shifted to high-temperature and this shift is associated with the thermal decomposition of carbides and oxides. Studies have shown that heating liquid pipe steel above the temperature of the liquid–liquid structural transition makes it possible to obtain a more homogeneous structure with a more uniform distribution of alloying and impurity elements in the melt. The sharp drop in surface tension at temperatures above 1920 K in the melt without vacuum degassing is associated with the diffusion of free S and O atoms, which are released after thermal decomposition of sulfides and oxides.
Research Progress of Cryogenic Materials for Storage and Transportation of Liquid Hydrogen
Authors: Qiu Yinan , Yang Huan , Tong Lige , Wang Li
Published: July 2021

Liquid hydrogen is the main fuel of large-scale low-temperature heavy-duty rockets, and has become the key direction of energy development in China in recent years. As an important application carrier in the large-scale storage and transportation of liquid hydrogen, liquid hydrogen cryogenic storage and transportation containers are the key equipment related to the national defense security of China’s aerospace and energy fields. Due to the low temperature of liquid hydrogen (20 K), special requirements have been put forward for the selection of materials for storage and transportation containers including the adaptability of materials in a liquid hydrogen environment, hydrogen embrittlement characteristics, mechanical properties, and thermophysical properties of liquid hydrogen temperature, which can all affect the safe and reliable design of storage and transportation containers. Therefore, it is of great practical significance to systematically master the types and properties of cryogenic materials for the development of liquid hydrogen storage and transportation containers. With the wide application of liquid hydrogen in different occasions, the requirements for storage and transportation container materials are not the same. In this paper, the types and applications of cryogenic materials commonly used in liquid hydrogen storage and transportation containers are reviewed. The effects of low-temperature on the mechanical properties of different materials are introduced. The research progress of cryogenic materials and low-temperature performance data of materials is introduced. The shortcomings in the research and application of cryogenic materials for liquid hydrogen storage and transportation containers are summarized to provide guidance for the future development of container materials. Among them, stainless steel is the most widely used cryogenic material for liquid hydrogen storage and transportation vessel, but different grades of stainless steel also have different applications, which usually need to be comprehensively considered in combination with its low temperature performance, corrosion resistance, welding performance, and other aspects. However, with the increasing demand for space liquid hydrogen storage and transportation, the research on high specific strength cryogenic materials such as aluminum alloy, titanium alloy, or composite materials is also developing. Aluminum alloy liquid hydrogen storage and transportation containers are widely used in the space field, while composite materials have significant advantages in being lightweight. Hydrogen permeation is the key bottleneck of composite storage and transportation containers. At present, there are still many technical problems that have not been solved.
Forming and Heat Treatment of Modern Metallic Materials
Authors: Moravec Ján , Gryc Karel
Published: July 2021

Forming and heat treatment of metals are crucial technologies for final metallic material processing at the end of production line, before they are incorporated into the final products or crucial components of more and more demanding systems required for the sustainability and development and of our civilization [...]
Early Postnatal Genistein Administration Affects Mice Metabolism and Reproduction in a Sexually Dimorphic Way
Authors: Marraudino Marilena , Ponti Giovanna , Moussu Chantal , Farinetti Alice , Macchi Elisabetta , Accornero Paolo , Gotti Stefano , Collado Paloma , Keller Matthieu , Panzica Giancarlo
Published: July 2021

The phytoestrogen genistein (GEN) may interfere with permanent morphological changes in the brain circuits sensitive to estrogen. Due to the frequent use of soy milk in the neonatal diet, we aimed to study the effects of early GEN exposure on some physiological and reproductive parameters. Mice of both sexes from PND1 to PND8 were treated with GEN (50 mg/kg body weight, comparable to the exposure level in babies fed with soy-based formulas). When adult, we observed, in GEN-treated females, an advanced pubertal onset and an altered estrous cycle, and, in males, a decrease of testicle weight and fecal testosterone concentration. Furthermore, we observed an increase in body weight and altered plasma concentrations of metabolic hormones (leptin, ghrelin, triiodothyronine) limited to adult females. Exposure to GEN significantly altered kisspeptin and POMC immunoreactivity only in females and orexin immunoreactivity in both sexes. In conclusion, early postnatal exposure of mice to GEN determines long-term sex-specific organizational effects. It impairs the reproductive system and has an obesogenic effect only in females, which is probably due to the alterations of neuroendocrine circuits controlling metabolism; thus GEN, should be classified as a metabolism disrupting chemical.
Optimal Method for Test and Repair Memories Using Redundancy Mechanism for SoC
Authors: Alnatheer Suleman , Ahmed Mohammed Altaf
Published: July 2021

The current system-on-chip (SoC)-based devices uses embedded memories of enormous size. Most of these systems’ area is dense with memories and promotes different types of faults appearance in memory. The memory faults become a severe issue when they affect the yield of the product. A memory-test and -repair scheme is an attractive solution to tackle this kind of problem. The built-in self-repair (BISR) scheme is a prominent method to handle this issue. The BISR scheme is widely used to repair the defective memories for an SoC-based system. It uses a built-in redundancy analysis (BIRA) circuit to allocate the redundancy when defects appear in the memory. The data are accessed from the redundancy allocation when the faulty memory is operative. Thus, this BIRA scheme affects the area overhead for the BISR circuit when it integrates to the SoC. The spare row and spare column–based BISR method is proposed to receive the optimal repair rate with a low area overhead. It tests the memories for almost all the fault types and repairs the memory by using spare rows and columns. The proposed BISR block’s performance was measured for the optimal repair rate and the area overhead. The area overhead, timing, and repair rate were compared with the other approaches. Furthermore, the study noticed that the repair rate and area overhead would increase by increasing the spare-row/column allocation.
Correction: Azinheiro et al. Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula. Microorganisms 2020, 8, 1359
Authors: Azinheiro Sarah , Carvalho Joana , Prado Marta , Garrido-Maestu Alejandro
Published: July 2021

The authors would like to make the following correction to the published paper [...]
The Late Jurassic–Palaeogene Carbonate Platforms in the Outer Western Carpathian Tethys—A Regional Overview
Authors: Kowal-Kasprzyk Justyna , Waśkowska Anna , Golonka Jan , Krobicki Michał , Skupien Petr , Słomka Tadeusz
Published: July 2021

The present work focuses on palaeogeographic reconstruction of shallow-water carbonate deposition in the Outer Western Carpathian Tethys. Platform deposits are preserved only as a component of turbidites and olistostromes, and reconstructions of these platforms are based on clastic material redistributed into slopes and deep basins and occurring among the Outer Carpathian nappes. Similar platforms were also present on the Tethys margins. These reconstructions were performed using the global models of plate tectonics. Several ridges covered by carbonate platforms developed in that area during the latest Jurassic–Palaeogene times. Three main shallow-water facies associations—Štramberk, Urgonian, and Lithothamnion–bryozoan—could be distinguished. The Tithonian–lowermost Cretaceous Štramberk facies is related to early, synrift–postrift stage of the development of the Silesian Domain. Facies that are diversified, narrow, shallow-water platforms, rich in corals, sponges, green algae, echinoderms, foraminifera, microencrusters, and microbes are typical of this stage. The Urgonian facies developed mainly on the south margin of the Outer Carpathian basins and is characterised by organodetritic limestones built of bivalves (including rudists), larger benthic foraminifera, crinoids, echinoids, and corals. Since the Paleocene, in all the Western Outer Carpathian sedimentary areas, Lithothamnion–bryozoan facies developed and adapted to unstable conditions. Algae–bryozoan covers originating on the siliciclastic substrate are typical of these facies. This type of deposition was preserved practically until the final stage in the evolution of the Outer Carpathian basins.
Enhanced Electrochemical Performance of Supercapacitors via Atomic Layer Deposition of ZnO on the Activated Carbon Electrode Material
Authors: Wu Chongrui , Zhang Fuming , Xiao Xiangshang , Chen Junyan , Sun Junqi , Gandla Dayakar , Ein-Eli Yair , Tan Daniel Q.
Published: July 2021

Fabricating electrical double-layer capacitors (EDLCs) with high energy density for various applications has been of great interest in recent years. However, activated carbon (AC) electrodes are restricted to a lower operating voltage because they suffer from instability above a threshold potential window. Thus, they are limited in their energy storage. The deposition of inorganic compounds’ atomic layer deposition (ALD) aiming to enhance cycling performance of supercapacitors and battery electrodes can be applied to the AC electrode materials. Here, we report on the investigation of zinc oxide (ZnO) coating strategy in terms of different pulse times of precursors, ALD cycles, and deposition temperatures to ensure high electrical conductivity and capacitance retention without blocking the micropores of the AC electrode. Crystalline ZnO phase with its optimal forming condition is obtained preferably using a longer precursor pulse time. Supercapacitors comprising AC electrodes coated with 20 cycles of ALD ZnO at 70 °C and operated in TEABF4/acetonitrile organic electrolyte show a specific capacitance of 23.13 F g−1 at 5 mA cm−2 and enhanced capacitance retention at 3.2 V, which well exceeds the normal working voltage of a commercial EDLC product (2.7 V). This work delivers an additional feasible approach of using ZnO ALD modification of AC materials, enhancing and promoting stable EDLC cells under high working voltages.
The Lysozyme Inhibitor Thionine Acetate Is Also an Inhibitor of the Soluble Lytic Transglycosylase Slt35 from Escherichia coli
Authors: Mezoughi Aysha B. , Costanzo Chiara M. , Parker Gregor M. , Behiry Enas M. , Scott Alan , Wood Andrew C. , Adams Sarah E. , Sessions Richard B. , Loveridge E. Joel
Published: July 2021

Lytic transglycosylases such as Slt35 from E. coli are enzymes involved in bacterial cell wall remodelling and recycling, which represent potential targets for novel antibacterial agents. Here, we investigated a series of known glycosidase inhibitors for their ability to inhibit Slt35. While glycosidase inhibitors such as 1-deoxynojirimycin, castanospermine, thiamet G and miglitol had no effect, the phenothiazinium dye thionine acetate was found to be a weak inhibitor. IC50 values and binding constants for thionine acetate were similar for Slt35 and the hen egg white lysozyme. Molecular docking simulations suggest that thionine binds to the active site of both Slt35 and lysozyme, although it does not make direct interactions with the side-chain of the catalytic Asp and Glu residues as might be expected based on other inhibitors. Thionine acetate also increased the potency of the beta-lactam antibiotic ampicillin against a laboratory strain of E. coli.
Colloidal Synthesis and Characterization of Molybdenum Chalcogenide Quantum Dots Using a Two-Source Precursor Pathway for Photovoltaic Applications
Authors: Chikukwa Evernice , Meyer Edson , Mbese Johannes , Zingwe Nyengerai
Published: July 2021

The drawbacks of utilizing nonrenewable energy have quickened innovative work on practical sustainable power sources (photovoltaics) because of their provision of a better-preserved decent environment which is free from natural contamination and commotion. Herein, the synthesis, characterization, and application of Mo chalcogenide nanoparticles (NP) as alternative sources in the absorber layer of QDSSCs is discussed. The successful synthesis of the NP was confirmed as the results from the diffractive peaks obtained from XRD which were positive and agreed in comparison with the standard. The diffractive peaks were shown in the planes (100), (002), (100), and (105) for the MoS2 nanoparticles; (002), (100), (103), and (110) for the MoSe2 nanoparticles; and (0002), (0004), (103), as well as (0006) for the MoTe2 nanoparticles. MoSe2 presented the smallest size of the nanoparticles, followed by MoTe2 and, lastly, by MoS2. These results agreed with the results obtained using SEM analysis. For the optical properties of the nanoparticles, UV–Vis and PL were used. The shift of the peaks from the red shift (600 nm) to the blue shift (270–275 nm and 287–289 nm (UV–Vis)) confirmed that the nanoparticles were quantum-confined. The application of the MoX2 NPs in QDSSCs was performed, with MoSe2 presenting the greatest PCE of 7.86%, followed by MoTe2 (6.93%) and, lastly, by MoS2, with the PCE of 6.05%.
Evaluation of the Health-Promoting Properties of Selected Fruits
Authors: Figueira José A. , Porto-Figueira Priscilla , Berenguer Cristina , Pereira Jorge A. M. , Câmara José S.
Published: July 2021

In this study, the health-promoting benefits of different fruits grown in Madeira Island, namely lemon (Citrus limon var. eureka), tangerine (Citrus reticulata var. setubalense), pitanga (Eugenia uniflora var. red), tomato (Solanum lycopersicum var. gordal) and uva-da-serra, an endemic blueberry (Vaccinium padifolium Sm.), were investigated. The phenolic composition (total phenolics and total flavonoids content) and antioxidant capacity (assessed through ABTS and DPPH assays) were measured revealing a high phenolic potential for all fruits, except tomato, while uva-da-serra is particularly rich in flavonoids. In relation to the antioxidant capacity, the highest values were obtained for pitanga and uva-da-serra extracts. The bioactive potential was also assessed through the ability of the extracts to inhibit digestive enzymes linked to diabetes (α-amylase, α- and β-glucosidases) and hypertension (angiotensin-converting enzyme, ACE). The results obtained point to a very high bioactive potential with the selected samples exhibiting very important ACE anti-enzymatic capacities. A statistical analysis of the obtained data reveals a very strong correlation between ABTS and TPC, and a strong contribution of the fruit polyphenols for enzyme inhibition, and thus, presenting high antihypertensive and antidiabetic capacities. Overall, the results obtained clearly show a high bioactive potential of the selected fruits that should be further studied, in terms of specific phenolic composition. Moreover, these results strongly support the valorisation of pitanga seeds usually discarded as a waste, and uva-da-serra, an endemic and wild bush, as potential bioresources of bioactive compounds with impact in human diet.
Chiral Polythiophenes: Part I: Syntheses of Monomeric Precursors
Authors: Krasowska Dorota , Karpowicz Rafał , Drabowicz Józef
Published: July 2021

The purpose of this mini-review is to comprehensively present the synthetic approaches used for the preparation of non-racemic mono- and multi-substituted thiophenes, which, in turn, can be applied as precursors for the synthesis of chiral polythiophenes isolated as a single chemical entity or having supramolecular thin-layer architectures.
Encapsulation of Fruit Flavor Compounds through Interaction with Polysaccharides
Authors: Buljeta Ivana , Pichler Anita , Ivić Ivana , Šimunović Josip , Kopjar Mirela
Published: July 2021

Production and storage, the influence of packaging materials and the presence of other ingredients in fruit products can cause changes in flavor compounds or even their loss. Due to these issues, there is a need to encapsulate flavor compounds, and polysaccharides are often used as efficient carriers. In order to achieve effective encapsulation, satisfactory retention and/or controlled release of flavor compounds, it is necessary to understand the nature of the coated and coating materials. Interactions that occur between these compounds are mostly non-covalent interactions (hydrogen bonds, hydrophobic interactions and van der Waals forces); additionally, the formation of the inclusion complexes of flavor compounds and polysaccharides can also occur. This review provides insight into studies about the encapsulation of flavor compounds, as well as basic characteristics of encapsulation such as the choice of coating material, the effect of various factors on the encapsulation efficiency and an explanation of the nature of binding.
Oxyresveratrol: Sources, Productions, Biological Activities, Pharmacokinetics, and Delivery Systems
Authors: Likhitwitayawuid Kittisak
Published: July 2021

Oxyresveratrol has recently attracted much research attention due to its simple chemical structure and diverse therapeutic potentials. Previous reviews describe the chemistry and biological activities of this phytoalexin, but additional coverage and greater accessibility are still needed. The current review provides a more comprehensive summary, covering research from 1955 to the present year. Oxyresveratrol occurs in both gymnosperms and angiosperms. However, it has never been reported in plants in the subclass Sympetalae, and this point might be of both chemotaxonomic and biosynthetic importance. Oxyresveratrol can be easily obtained from plant materials by conventional methods, and several systems for both qualitative and quantitative analysis of oxyresveratrol contents in plant materials and plant products are available. Oxyresveratrol possesses diverse biological and pharmacological activities such as the inhibition of tyrosinase and melanogenesis, antioxidant and anti-inflammatory activities, and protective effects against neurological disorders and digestive ailments. However, the unfavorable pharmacokinetic properties of oxyresveratrol, including low water solubility and poor oral availability and stability, have posed challenges to its development as a useful therapeutic agent. Recently, several delivery systems have emerged, with promising outcomes that may improve chances for the clinical study of oxyresveratrol.
The Creation of True Two-Dimensional Silicon Carbide
Authors: Chabi Sakineh , Guler Zeynel , Brearley Adrian J. , Benavidez Angelica D. , Luk Ting Shan
Published: July 2021

This paper reports the successful synthesis of true two-dimensional silicon carbide using a top-down synthesis approach. Theoretical studies have predicted that 2D SiC has a stable planar structure and is a direct band gap semiconducting material. Experimentally, however, the growth of 2D SiC has challenged scientists for decades because bulk silicon carbide is not a van der Waals layered material. Adjacent atoms of SiC bond together via covalent sp3 hybridization, which is much stronger than van der Waals bonding in layered materials. Additionally, bulk SiC exists in more than 250 polytypes, further complicating the synthesis process, and making the selection of the SiC precursor polytype extremely important. This work demonstrates, for the first time, the successful isolation of 2D SiC from hexagonal SiC via a wet exfoliation method. Unlike many other 2D materials such as silicene that suffer from environmental instability, the created 2D SiC nanosheets are environmentally stable, and show no sign of degradation. 2D SiC also shows interesting Raman behavior, different from that of the bulk SiC. Our results suggest a strong correlation between the thickness of the nanosheets and the intensity of the longitudinal optical (LO) Raman mode. Furthermore, the created 2D SiC shows visible-light emission, indicating its potential applications for light-emitting devices and integrated microelectronics circuits. We anticipate that this work will cause disruptive impact across various technological fields, ranging from optoelectronics and spintronics to electronics and energy applications.
Optimizing the Properties of La0.8Sr0.2CrO3 Thin Films through Post-Annealing for High-Temperature Sensing
Authors: Liu Dan , Shi Peng , Liu Yantao , Zhang Yijun , Tian Bian , Ren Wei
Published: July 2021

La0.8Sr0.2CrO3 (0.2LSCO) thin films were prepared via the RF sputtering method to fabricate thin-film thermocouples (TFTCs), and post-annealing processes were employed to optimize their properties to sense high temperatures. The XRD patterns of the 0.2LSCO thin films showed a pure phase, and their crystallinities increased with the post-annealing temperature from 800 °C to 1000 °C, while some impurity phases of Cr2O3 and SrCr2O7 were observed above 1000 °C. The surface images indicated that the grain size increased first and then decreased, and the maximum size was 0.71 μm at 1100 °C. The cross-sectional images showed that the thickness of the 0.2LSCO thin films decreased significantly above 1000 °C, which was mainly due to the evaporation of Sr2+ and Cr3+. At the same time, the maximum conductivity was achieved for the film annealed at 1000 °C, which was 6.25 × 10−2 S/cm. When the thin films post-annealed at different temperatures were coupled with Pt reference electrodes to form TFTCs, the trend of output voltage to first increase and then decrease was observed, and the maximum average Seebeck coefficient of 167.8 µV/°C was obtained for the 0.2LSCO thin film post-annealed at 1100 °C. Through post-annealing optimization, the best post-annealing temperature was 1000 °C, which made the 0.2LSCO thin film more stable to monitor the temperatures of turbine engines for a long period of time.
Non-Coding, RNAPII-Dependent Transcription at the Promoters of rRNA Genes Regulates Their Chromatin State in S. cerevisiae
Authors: Lesage Emma , Perez-Fernandez Jorge , Queille Sophie , Dez Christophe , Gadal Olivier , Kwapisz Marta
Published: July 2021

Pervasive transcription is widespread in eukaryotes, generating large families of non-coding RNAs. Such pervasive transcription is a key player in the regulatory pathways controlling chromatin state and gene expression. Here, we describe long non-coding RNAs generated from the ribosomal RNA gene promoter called UPStream-initiating transcripts (UPS). In yeast, rDNA genes are organized in tandem repeats in at least two different chromatin states, either transcribed and largely depleted of nucleosomes (open) or assembled in regular arrays of nucleosomes (closed). The production of UPS transcripts by RNA Polymerase II from endogenous rDNA genes was initially documented in mutants defective for rRNA production by RNA polymerase I. We show here that UPS are produced in wild-type cells from closed rDNA genes but are hidden within the enormous production of rRNA. UPS levels are increased when rDNA chromatin states are modified at high temperatures or entering/leaving quiescence. We discuss their role in the regulation of rDNA chromatin states and rRNA production.
Assessment of the Risk of Contamination of Food for Infants and Toddlers
Authors: Mielech Anita , Puścion-Jakubik Anna , Socha Katarzyna
Published: July 2021

Infants and toddlers are highly sensitive to contaminants in food. Chronic exposure can lead to developmental delays, disorders of the nervous, urinary and immune systems, and to cardiovascular disease. A literature review was conducted mainly in PubMed, Google Scholar and Scopus databases, and took into consideration papers published from October 2020 to March 2021. We focused on contaminant content, intake estimates, and exposure to contaminants most commonly found in foods consumed by infants and children aged 0.5–3 years. In the review, we included 83 publications with full access. Contaminants that pose a high health risk are toxic elements, acrylamide, bisphenol, and pesticide residues. Minor pollutants include: dioxins, mycotoxins, nitrates and nitrites, and polycyclic aromatic hydrocarbons. In order to reduce the negative health effects of food contamination, it seems reasonable to educate parents to limit foods that are potentially dangerous for infants and young children. An appropriate varied diet, selected cooking techniques, and proper food preparation can increase the likelihood that the foods children consume are safe for their health. It is necessary to monitor food contamination, adhere to high standards at every stage of production, and improve the quality of food for children.
Effects of Hormone Therapy and Flavonoids Capable on Reversal of Menopausal Immune Senescence
Authors: Vrachnis Nikolaos , Zygouris Dimitrios , Vrachnis Dionysios , Antonakopoulos Nikolaos , Fotiou Alexandros , Panagopoulos Periklis , Kolialexi Aggeliki , Pappa Kalliopi , Mastorakos George , Iliodromiti Zoi
Published: July 2021

Menopause, probably the most important natural change in a woman’s life and a major component of female senescence, is characterized, inter alia, by cessation of ovarian estrogen and progesterone production, resulting in a gradual deterioration of the female immune system. Hormone replacement therapy (HRT) is used in postmenopausal women to relieve some of the peri- and postmenopausal symptoms, while there is also evidence that the therapy may additionally partially reverse menopausal immune senescence. Flavonoids, and especially isoflavones, are widely used for the treatment of menopausal symptoms, although it is not at present clear whether they can reverse or alleviate other menopausal changes. HRT reverses the menopausal CD4/CD8 ratio and also limits the general peri- and postmenopausal inflammatory state. Moreover, the increased levels of interleukins (IL)-1β, IL-6, and IL-8, as well as of tumor necrosis factor-α (TNF-α) are decreased after the initiation of HRT. However, some reports show no effect of HRT on IL-4, IL-10, and IL-12. It is thus evident that the molecular pathways connecting HRT and female immune senescence need to be clarified. Interestingly, recent studies have suggested that the anti-inflammatory properties of isoflavones possibly interact with inflammatory cytokines when applied in menopause treatments, thereby potentially reversing immune senescence. This narrative review presents the latest data on the effect of menopausal therapies, including administration of flavonoid-rich products, on age-associated immune senescence reversal with the aim of revealing possible directions for future research and treatment development.
Ex Vivo Evaluation of the Sepsis Triple Therapy High-Dose Vitamin C in Combination with Vitamin B1 and Hydrocortisone in a Human Peripheral Blood Mononuclear Cells (PBMCs) Model
Authors: Lauer Annie , Burkard Markus , Niessner Heike , Leischner Christian , Renner Olga , Vollbracht Claudia , Michels Holger , Busch Christian , Sinnberg Tobias , Venturelli Sascha
Published: July 2021

Sepsis is an extremely complex clinical syndrome, usually involving an excessive inflammatory response including an overshooting cytokine release that damages tissue and organs of the patient. Due to the severity of this condition, it is estimated that over 11 million people die from sepsis each year. Despite intensive research in the field, there is still no specific therapy for sepsis. Many sepsis patients show a marked deficiency of vitamin C. 9 out of 10 sepsis patients have a hypovitaminosis C, and every third patient even shows a clinical deficiency in the scurvy range. In addition, low vitamin C levels of intensive care sepsis patients correlate with a higher need for vasopressors, higher Sequential Organ Failure Assessment (SOFA) scores, and increased mortality. Based on this observation and the conducted clinical trials using vitamin C as sepsis therapy in intensive care patients, the aim of the present ex vivo study was to evaluate the effects of high-dose vitamin C alone and in a triple combination supplemented with vitamin B1 (thiamine) and hydrocortisone on the lipopolysaccharide (LPS)-induced cytokine response in peripheral blood mononuclear cells (PBMCs) from healthy human donors. We found that all corticosteroid combinations strongly reduced the cytokine response on RNA- and protein levels, while high-dose vitamin C alone significantly diminished the PBMC mediated secretion of the cytokines interleukin (IL)-10, IL-23, and monocyte chemo-attractant protein (MCP-1), which mediate the inflammatory response. However, vitamin C showed no enhancing effect on the secretion of further cytokines studied. This data provides important insights into the possible immunomodulatory function of vitamin C in an ex vivo setting of human PBMCs and the modulation of their cytokine profile in the context of sepsis. Since vitamin C is a vital micronutrient, the restoration of physiologically adequate concentrations should be integrated into routine sepsis therapy, and the therapeutic effects of supraphysiological concentrations of vitamin C in sepsis patients should be further investigated in clinical trials.
Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice
Authors: Rosell-Cardona Cristina , Griñan-Ferré Christian , Pérez-Bosque Anna , Polo Javier , Pallàs Mercè , Amat Concepció , Moretó Miquel , Miró Lluïsa
Published: July 2021

Alzheimer’s disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and the accumulation of hyperphosphorylated tau, both of which are accompanied by neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in inflammation models. We investigated whether dietary supplementation with SDP prevents the neuropathological features of AD. The experiments were performed in 2- and 6-month-old SAMP8 mice fed a control diet, or a diet supplemented with 8% SDP, for 4 months. AD brain molecular markers were determined by Western blot and real-time PCR. Senescent mice showed reduced levels of p-GSK3β (Ser9) and an increase in p-CDK5, p-tau (Ser396), sAPPβ, and the concentration of Aβ40, (all p < 0.05). SDP prevented these effects of aging and reduced Bace1 levels (all p < 0.05). Senescence increased the expression of Mme1 and Ide1 and pro-inflammatory cytokines (Il-17 and Il-18; all p < 0.05); these changes were prevented by SDP supplementation. Moreover, SDP increased Tgf-β expression (p < 0.05). Furthermore, in aged mice, the gene expression levels of the microglial activation markers Trem2, Ym1, and Arg1 were increased, and SDP prevented these increases (all p < 0.05). Thus, dietary SDP might delay AD onset by reducing its hallmarks in senescent mice.
The Validity of Ultrasound Technology in Providing an Indirect Estimate of Muscle Glycogen Concentrations Is Equivocal
Authors: Bone Julia L. , Ross Megan L. , Tomcik Kristyen A. , Jeacocke Nikki A. , McKay Alannah K. A. , Burke Louise M.
Published: July 2021

Researchers and practitioners in sports nutrition would greatly benefit from a rapid, portable, and non-invasive technique to measure muscle glycogen, both in the laboratory and field. This explains the interest in MuscleSound®, the first commercial system to use high-frequency ultrasound technology and image analysis from patented cloud-based software to estimate muscle glycogen content from the echogenicity of the ultrasound image. This technique is based largely on muscle water content, which is presumed to act as a proxy for glycogen. Despite the promise of early validation studies, newer studies from independent groups reported discrepant results, with MuscleSound® scores failing to correlate with the glycogen content of biopsy-derived mixed muscle samples or to show the expected changes in muscle glycogen associated with various diet and exercise strategies. The explanation of issues related to the site of assessment do not account for these discrepancies, and there are substantial problems with the premise that the ratio of glycogen to water in the muscle is constant. Although further studies investigating this technique are warranted, current evidence that MuscleSound® technology can provide valid and actionable information around muscle glycogen stores is at best equivocal.
Characterization of a Dengue Virus Serotype 1 Isolated from a Patient in Ciudad Juarez, Mexico
Authors: Palermo Pedro M. , de la Mora-Covarrubias Antonio , Orbegozo Jeanette , Plante Jessica A. , Plante Kenneth S. , Jimenez-Vega Florinda , Watts Douglas M.
Published: July 2021

Dengue (DEN) is the most important human arboviral disease worldwide. Sporadic outbreaks of DEN have been reported since 1980 in urban communities located along the border in southeast Texas and northern Mexico. Other than the Rio Grande Valley region of TX, autochthonous transmission of DENV has not been reported from any other US border communities. As part of a surveillance program for arthropod-borne viruses in Ciudad Juarez, Mexico, during November 2015, a blood sample was obtained from a female patient who experienced an undifferentiated fever and arthralgia. The plasma of the sample was tested for virus in Vero-76 and C6/36 cells. DENV serotype 1 (DENV-1) was isolated in the C6/36 cells, and nucleotide sequencing of the envelope gene and full genome grouped the DENV-1 isolate in the Central America clade. The patient had not traveled outside of Ciudad Juarez, Mexico, thus suggesting DENV-1 infection was acquired in this community.
Comparative EPR Study on the Scavenging Effect of Methotrexate with the Isomers of Its Photoswitchable Derivative
Authors: Preisz Zsolt , Hartvig Nóra , Bognár Balázs , Kálai Tamás , Kunsági-Máté Sándor
Published: July 2021

The scavenging effect of the antimetabolite dihydrofolate reductase inhibitor methotrexate (MTX) and the isomers of its photoswitchable derivate, cis- and trans-phototrexate (PHX), have been compared by ESR spectroscopy, with the application of a cyclic hydroxylamine spin probe. The results showed the most pronounced scavenging effect in the presence of trans-phototrexate (trans-PHX). At a low concentration (100 µM) cis-PHX also showed a greater scavenging effect than the parent molecule MTX. Direct antioxidant properties of the investigated molecules were measured by ABTS scavenging assay, which showed no significant difference between trans-PHX and cis-PHX, but both of the isomers of PHX showed a higher antioxidant capacity than MTX. These findings imply that trans-PHX may have more pronounced anti-inflammatory and tissue-protective effects than MTX, despite the lack of its cytotoxic, antineoplastic effect.