bluuebunny/crossref_metadata_2025 huggingface dataset:
Thermoreversible Fibrous Hydrogel of Benzene‐Centered Tris‐dodecylimidazolium Bromide: A Dual Role as Stabilizer and Directing Agent for Silver Dendrites.
Authors: Rondla Rohini , Malikanti Ramesh , Puchakayala Muralidhar Reddy
Published: July 2019

Benzene centered tris‐alkylimidazolium bromide salts, denoted as BTIm(R)3Br3 ; where R=C12H25 and C14H29 have been prepared and structurally characterised. The hydrogelation ability and stabilizer role of these salts for the synthesis of silver nanoparticles (AgNPs) via chemical reduction method is described. A worth note that the BTIm(C12)3Br3 compound could lead a thermoreversible fibrous‐hydrogel in water, which plays a dual role as stabilizer and directing agent for silver dendritic nanostructures. While the partial‐hydrogel of BTIm(C12)3Br3 contributes silver nanospheres. The non‐gel solutions of BTIm(C12)3Br3 in dichloromethane, and BTIm(C14)3Br3 in water as stabilizers did not show control over the shape of AgNPs, and they lead mixed morphology. The experimental conditions, i. e. temperature, heating‐cooling process and the physical state of the stabilizer play a key role in the thermoreversible hydrogel and partial‐hydrogel control over the morphology of Ag nanostructures. Overall, the outcome of the present study provides new possibility for the use of thermoreversible fibrous gels to prepare Ag dendrites.
Regulation of Interfacial Charge Transfer and Recombination for Efficient Planar Perovskite Solar Cells
Authors: Shi Xiaoqiang , Chen Ruochen , Jiang Tingting , Ma Shuang , Liu Xuepeng , Ding Yong , Cai Molang , Wu Jihuai , Dai Songyuan
Published: July 2019

Control of dynamics at the electron transport layer–perovskite interface, such as charge transfer and recombination, is essential in achieving high‐efficiency planar perovskite solar cells (PSCs). Herein, it was observed that the trade‐off between unfavorable electron transport of a thick SnO2 film and serious electron recombination at thin SnO2 film/perovskite interfaces is essential for the performance of SnO2‐based planar PSCs. The optimized efficiency of devices beyond 20% is obtained by using a two‐step deposition of SnO2. Moreover, trap‐assisted carrier recombination is significantly suppressed by using the diethylenetriaminepentaacetic acid passivator via the formation of coordination with undercoordinated Sn and Pb2+ ions. As a result, the champion device demonstrates a promising efficiency of 21.28% with negligible hysteresis and much improved environmental stability, i.e., retaining 98% of the initial efficiency under ambient atmosphere over 1000 h.
Quasistructural molecules
Authors: Császár Attila G. , Fábri Csaba , Sarka János
Published: July 2019

The concept of quasistructural molecules is introduced. For quasistructural molecules (a) the notion of a static equilibrium structure, corresponding to a minimum on the potential energy surface of the molecule, loses its strict meaning, (b) internal nuclear motions (rotations and vibrations) become dominant, resulting in an effective molecular structure often even qualitatively different from the equilibrium one, (c) separation of the internal nuclear motions breaks down, rotational and vibrational degrees of freedom cannot be separated from each other when interpreting even the lowest rovibrational eigenstates of the molecule, often resulting in effective rotational constants drastically different from the equilibrium ones even for the ground vibrational eigenstate, (d) classification of the rovibrational states requires the use of permutation‐inversion symmetry and molecular‐symmetry groups, and (e) some of the rovibrational eigenenergies assigned to a vibrational parent state exhibit unconventional (in the most striking cases “negative”) rotational contributions. Molecules showing quasistructural behavior include neutral species, such as dimethyl acetylene, charged species, such as and , van der Waals complexes, such as CH4·H2O, and molecular complexes held together by halogen bonds, like CF3Cl·CH3F.

This article is categorized under:

Structure and Mechanism > Molecular Structures

Theoretical and Physical Chemistry > Spectroscopy

Software > Quantum Chemistry
Research on multifractal dimension and improved gray relation theory for intelligent satellite signal recognition
Authors: Zhang Zhen , Li Yibing , Qi Lin
Published: July 2019

In the wake of the development and advancement of signal processing technology for communication radiation source individual, Signal fingerprint feature extraction and analysis technology for communication radiation source individual has broad application prospects in many fields.
To effectively extract the individual characteristics of different modulated signals under low SNR environment, and recognize the subtle features for communication radiation source individuals has been a hot spot. Aiming at the problem of signal feature extraction and classifier design under low SNR environment, in the paper, a multifractal dimension and improved gray relation theory based classifier design algorithm is proposed. Firstly, the multifractal dimension feature extraction of nine modulated communication signals is realized. Then multifractal dimension features of these modulated signals under different SNR are compared. An improved gray relation algorithm is used to recognize the extracted subtle characteristics. Meanwhile, FSK signal is used to simulate radio subtle features by adding different distribution of noise. Subtle feature extraction by means of multifractal dimension algorithm and pattern recognition by means of improved gray relation algorithm are used to test the effectiveness of the proposed method for identification of modulated signals and radio subtle features. The simulation results show that the recognition success rate of nine different communication modulated signals can reach 93% even under the SNR of 2 dB, and the recognition success rate of the subtle features of distributed noise can reach 100%. The proposed method provides an effective theoretical basis for identifying of radio modulated signals and communication radiation source individual subtle features.
Knowledge of Cervical Cancer, Human Papilloma Virus (HPV) and HPV Vaccination Among Women in Northeast China
Authors: Ning Yu-e , Liu Yao , Xu Xiao-yu , Zhang Xin-yu , Wang Ning , Zheng Li-qiang
Published: July 2019

This study aimed to research the understanding and knowledge of cervical cancer, human papilloma virus (HPV), and HPV vaccination, and the acceptance of HPV vaccination, among a population of women in northeastern China. A cross-sectional survey was carried out by questionnaire to investigate knowledge of cervical cancer, HPV, and HPV vaccination. The 230 female participants were native residents of northeastern China, and their ages ranged between 18 and 65 years. Questionnaires were randomly acquired by the respondents from online and paper questionnaire distribution. The questionnaire included questions on three major aspects to record people’s perceptions of cervical cancer, HPV, and vaccines. Of the sample of 230 women surveyed, 80.9% had heard of cervical cancer, but understanding was only 15.7%; 38.3% knew about HPV; 20% knew about HPV vaccine; 39.6% agreed to receive HPV vaccination, and the remainder were mainly concerned about its safety and effectiveness. Data analysis showed that age, family income, and whether there was experience of screening all influenced knowledge of cervical cancer, but this was not statistically significant. The level of education had no obvious effect on the degree of knowledge about cervical cancer; however, with an improvement in education, women’s awareness of HPV vaccine improved significantly (p < 0.05). Women who have received cervical cancer screening had significantly greater knowledge about cervical cancer and HPV than those with no screening (p < 0.05). Women in northeastern China have little knowledge of cervical cancer, HPV, and HPV vaccine, lack disease knowledge, and hold a skeptical attitude about HPV vaccination. Medical institutions are the main channel providing information to these women.
The Interaction between Surfactants and Montmorillonite and its Influence on the Properties of Organo-Montmorillonite in Oil-Based Drilling FluIDS
Authors: Zhuang Guanzheng , Zhang Zepeng , Peng Shanmao , Gao Jiahua , Pereira Francisco A. R. , Jaber Maguy
Published: July 2019

The increasing demands for oil and gas and associated difficult drilling operations require oil-based drilling fluids that possess excellent rheological properties and thermal stability. The objective of the present work was to investigate the rheological properties and thermal stability of organo-montmorillonite (OMnt) modified with various surfactants and under various loading levels in oil-based drilling fluids, as revealed by the interaction between organic surfactants and montmorillonite. The influence of the structural arrangement of surfactants on the thermal stability of organo-montmorillonite (OMnt) in oil-based drilling fluids was also addressed. OMnt samples were prepared in aqueous solution using surfactants possessing either a single long alkyl chain two long alkyl chains. OMnt samples were characterized by X-ray diffraction, high-resolution transmission electron microscopy, thermal analysis, and X-ray photoelectron spectroscopy. Organic surfactants interacted with montmorillonite by electrostatic attraction. The arrangements of organic surfactants depended on the number of long alkyl chains and the geometrical shape of organic cations. In addition to the thermal stability of surfactants, intermolecular interaction also improved the thermal stability of OMnt/oil fluids. A tight paraffin-type bilayer arrangement contributed to the excellent rheological properties and thermal stability of OMnt/oil fluids. The deterioration of rheological properties of OMnt/oil fluids at temperatures up to 200°C was due mainly to the release of interlayer surfactants into the oil.
Longitudinal relations between future planning and adolescents’ academic achievement in China
Authors: Zhao Jingxin , Li Ruijuan , Ma Jinling , Zhang Wenxin
Published: July 2019

Introduction

Planning for the future is important for adolescents' development and academic adaptation. Most prior research has focused primarily on the effects of future planning on adolescents' academic achievement, and there is a dearth of literature examining the effects of adolescents' academic achievement on their future planning. The current study examined the longitudinal reciprocal relations between adolescents’ future planning and academic achievement in China.

Methods

The study sample consisted of 775 Chinese junior high school students (391 boys, 384 girls; Mage = 13.38 years, SD = 0.89). Future planning and academic achievement were measured at three time points that were equally spaced by a 6‐month lag. Path analyses with cross‐lagged effects were used to examine the longitudinal reciprocal relations between adolescents’ future planning and academic achievement.

Results

The associations between future educational planning and academic achievement were reciprocal. Future occupational commitment predicted adolescents' academic achievement, and academic achievement predicted adolescents’ affects concerning future occupation. However, there was no significant association between future occupational exploration and academic achievement. Moreover, these patterns of longitudinal relations applied equally to boys and girls, and younger and older adolescents.

Conclusions

The reciprocal relations between future planning processes and academic achievement differed across domains (e.g., educational and occupational domains). These findings suggest that future planning may be a protective factor for improving adolescents' academic achievement, and high academic achievement may also be beneficial for Chinese adolescents’ future planning.
3D Printing and Intellectual Property
Authors: Osborn Lucas S.
Published: July 2019

Intellectual property (IP) laws were drafted for tangible objects, but 3D printing technology, which digitizes objects and offers manufacturing capacity to anyone, is disrupting these laws and their underlying policies. In this timely work, Lucas S. Osborn focuses on the novel issues raised for IP law by 3D printing for the major IP systems around the world. He specifically addresses how patent and design law must wrestle with protecting digital versions of inventions and policing individualized manufacturing, how trademark law must confront the dissociation of design from manufacturing, and how patent and copyright law must be reconciled when digital versions of primarily utilitarian objects are concerned. With an even hand and keen insight, Osborn offers an innovation-centered analysis of and balanced response to the disruption caused by 3D printing that should be read by nonexperts and experts alike.
Comparing the Teaching of Comparative Law: A View from Singapore
Authors: JAMAL Arif A
Published: July 2019

This article explores what makes the teaching of comparative law distinctive as well as familiar in Singapore (and Southeast Asia more broadly), and so contributes to a process of comparing the teaching of comparative law. I argue that one must balance both familiarity and distinctiveness when teaching comparative law in Southeast Asia. This means that, on the one hand, we can indeed draw on general and even classical materials when teaching comparative law in this region. On the other hand, there is the need to address specific features that shape the context of Southeast Asia. Finally, I discuss how the Southeast Asian example may be instructive because of this balancing in highlighting the importance of teaching comparative law in other parts of the world.
Roles of Risk Managers: Understanding How Risk Managers Engage in Regulation
Authors: VAN DER VOORT Haiko , DE BRUIJNE Mark , STEENHUISEN Bauke
Published: July 2019

Inside companies that produce significant risks, risk managers play a key role. They manage the connection between the risk regulation regime, which stresses public values, and the company, which pursues a broader array of organisational goals. This makes the role of risk managers ambivalent. To better understand this ambivalence and identify the means, motives and strategies that risk managers employ in response to this ambivalence, this article conducts a concise review of (classic) organisation and regulatory literature. Based on this review, we propose a typology that distinguishes four roles of risk managers: risk managers as supporting staff; risk managers as professionals; risk managers as boundary spanners; and risk managers as agents in regulatory communities. Each type subsequently describes how risk managers employ different strategies in their attempt to connect the risk regulation regime and the company, ie translating policies to practices, tailoring policies to practices, explaining and framing policies and practices, and (re)interpreting policies and practices together with regulators. The typology enables researchers and practioners to emphasise and more thoroughly analyse the variety and complexity of risk managers’ work, and can help regulators to broaden and fine-tune their strategies to improve connections with the various roles of risk managers.
From Integration to Exclusion: EU Composite Administration and Gaps in Judicial Accountability in the Authorisation of Pharmaceuticals
Authors: RÖTTGER-WIRTZ Sabrina , ELIANTONIO Mariolina
Published: July 2019

In the EU, pharmaceuticals are subject to a marketing authorisation requirement that, depending on the type of product concerned, can be obtained either centrally through a Commission decision with EU-wide effects or in the Member States, potentially subject to mutual recognition in composite authorisation procedures. These composite decision-making processes can nowadays be considered a standard way of administrative decision-making in the EU internal market. Yet, judicial supervision remains anchored in the separation of jurisdictions between the national and the EU level, and between national levels. This article explores the challenges posed to judicial review in the case of pharmaceutical marketing authorisations when European administrative composite structures are used.
Teamwork and safety climate affect antimicrobial stewardship for asymptomatic bacteriuria
Authors: Drekonja Dimitri M. , Grigoryan Larissa , Lichtenberger Paola , Graber Christopher J. , Patel Payal K. , Van John N. , Dillon Laura M. , Wang Yiqun , Gauthier Timothy P. , Wiseman Steve W. , Shukla Bhavarth S. , Naik Aanand D. , Hysong Sylvia J. , Kramer Jennifer R. , Trautner Barbara W.
Published: July 2019

Objective:

In preparation for a multisite antibiotic stewardship intervention, we assessed knowledge and attitudes toward management of asymptomatic bacteriuria (ASB) plus teamwork and safety climate among providers, nurses, and clinical nurse assistants (CNAs).

Design:

Prospective surveys during January–June 2018.

Setting:

All acute and long-term care units of 4 Veterans’ Affairs facilities.

Methods:

The survey instrument included 2 previously tested subcomponents: the Kicking CAUTI survey (ASB knowledge and attitudes) and the Safety Attitudes Questionnaire (SAQ).

Results:

A total of 534 surveys were completed, with an overall response rate of 65%. Cognitive biases impacting management of ASB were identified. For example, providers presented with a case scenario of an asymptomatic patient with a positive urine culture were more likely to give antibiotics if the organism was resistant to antibiotics. Additionally, more than 80% of both nurses and CNAs indicated that foul smell is an appropriate indication for a urine culture. We found significant interprofessional differences in teamwork and safety climate (defined as attitudes about issues relevant to patient safety), with CNAs having highest scores and resident physicians having the lowest scores on self-reported perceptions of teamwork and safety climates (P < .001). Among providers, higher safety-climate scores were significantly associated with appropriate risk perceptions related to ASB, whereas social norms concerning ASB management were correlated with higher teamwork climate ratings.

Conclusions:

Our survey revealed substantial misunderstanding regarding management of ASB among providers, nurses, and CNAs. Educating and empowering these professionals to discourage unnecessary urine culturing and inappropriate antibiotic use will be key components of antibiotic stewardship efforts.
Breaking Even: Political Economy and Private Enterprise in the Norwegian Glass Industry, 1739–1803
Authors: Amdam Rolv Petter , Fredona Robert , Reinert Sophus A.
Published: July 2019

Using internal debates and surviving account books, this article traces the eighteenth-century history of the Norwegian glass industry, created to exploit Norway's immense natural resource wealth, and of the chartered company that would later become Norway's iconic Christiania Glasmagasin. The investors in the company, many of them among Norway's “founding fathers,” were individually responsible for its losses and it operated, remarkably, at an annual loss for nearly five decades. The article asks why, beyond the anticipation of a royal import ban on foreign glass, private investors might have continued to accept such losses. It focuses on tensions between cameralist and liberal ideologies in the creation of an important national industry, and on older (and perhaps more sustainable) ways of thinking about profitability.
The German Textile Puzzle: Selective Protectionism and the Silent Globalization of an Industry
Authors: Hesse Jan-Otmar
Published: July 2019

As in other countries, textile and apparel production in Germany is considered a victim of globalization. Domestic production and employment declined dramatically after its postwar peak in the late 1950s. Research has often attributed this trajectory to the trade liberalization policy of the German governments. However, this interpretation is puzzling. German trade policy was not as liberal as is claimed, nor did the industry disappear. This article addresses the issue using statistical evidence as well as archival material. The West German textile and apparel industry was using outward processing strategies comparatively early and was supported in that by German politicians starting in the early 1960s. As a result, the industry moved up the global value chain of textile production.
Age heaping among adults in Nigeria: evidence from the Nigeria Demographic and Health Surveys 2003–2013
Authors: Fayehun Olufunke , Ajayi Anthony I. , Onuegbu Chinwe , Egerson Daniel
Published: July 2019

Age, as a variable, represents a critical basis for demographic classification; thus, its misrepresentations or misreporting alter the accuracy of demographic estimates. This paper examines the extent and pattern of age heaping in the age data for adults, collected in the Nigerian Demographic Health Survey (NDHS). The study used the NDHS data for 2003, 2008, and 2013 to compute a Whipple’s and Meyers’ blended index for each survey year, by gender, geopolitical zones, states and place of residence. The analysis shows that age heaping was higher than the acceptable levels in all three data sets. The Whipple’s index puts the rate of age heaping in the 2003 dataset at 271.3, whilst the rates declined slightly in the 2008 and 2013 datasets to reach 204.2 and 202.5 respectively. Similarly, the Myers’ blended index portrayed that age heaping in the 2003 data was highest at 47.0 while the subsequent years were lower at 38.60 and 38.66, respectively. Digits ending in 0 and 5 were mostly reported in all three surveys and higher rates of age heaping were observed among males, the uneducated and rural dwellers. Age heaping was prominent in all three surveys, thus affecting the data quality gathered at these surveys. Thus, future studies should assess the extent to which age misreporting could bias the estimate of fertility rate.
Measuring and explaining formal institutional persistence in French West Africa
Authors: Berinzon Maya , Briggs Ryan C.
Published: July 2019

Colonial institutions are thought to be highly persistent, but measuring that persistence is difficult. Using a text analysis method that allows us to measure similarity between bodies of text, we examine the extent to which one formal institution – the penal code – has retained colonial language in seven West African countries. We find that the contemporary penal codes of most countries retain little colonial language. Additionally, we find that it is not meaningful to speak of institutional divergence across the unit of French West Africa, as there is wide variation in the legislative post-coloniality of individual countries. We present preliminary analyses explaining this variation and show that the amount of time that a colony spent under colonisation correlates with more persistent colonial institutions.
Point-to-Point Motion Planning of a Free-Floating Space Manipulator Using the Rapidly-Exploring Random Trees (RRT) Method
Authors: Rybus Tomasz
Published: July 2019

It is usually proposed to use a robotic manipulator for performing on-orbit capture of a target satellite in the planned active debris removal and on-orbit servicing missions. Control of the satellite-manipulator system is challenging because motion of the manipulator influences position and orientation of the chaser satellite. Moreover, the trajectory selected for the capture manoeuvre must be collision-free. In this article, we consider the case of a nonredundant manipulator mounted on a free-floating satellite.We propose to use the bi-directional rapidly-exploring random trees (RRT) algorithm to achieve two purposes: to plan a collision-free manipulator trajectory that, at the same time, will result in a desired change of the chaser satellite orientation. Several improvements are introduced in comparison to the previous applications of the RRT method for manipulator mounted on a free-floating satellite. Feasibility of the proposed approach is demonstrated in numerical simulations performed for the planar case in which the chaser satellite is equipped with a 2-DoF (Degree of Freedom) manipulator. The obtained results are analysed and compared with the results obtained from collision-free trajectory planning methods that do not allow to set the desired final orientation of the chaser satellite.
WILLIAM J. BARBER ON IRVING FISHER AND AMERICAN ECONOMIC THOUGHT
Authors: Dimand Robert W.
Published: July 2019

The eminent scholar William J. Barber made three great contributions to the history of American economic thought: on the institutionalization of political economy in US universities in the nineteenth century, on the interplay between economic ideas and events in US policy-making from 1921 to 1945, and on the contributions, writings, and career of Irving Fisher.
WTO/GATS-Alien Framework Provisions in RTAs – A Closer Look
Authors: Adlung Rudolf
Published: July 2019

This article deals with a challenge in drafting regional trade agreements (RTAs) in services that has been widely ignored to date: the need to comply with, and ensure consistent use of, GATS-based framework provisions. This includes in particular the requirement for the RTA parties to eliminate substantially all discrimination between their services and service suppliers. Yet the criteria that are used in individual agreements to identify the benchmark services and service suppliers quite often depart from relevant GATS provisions (Article V on economic integration in combination with Article XVII on national treatment). These departures may not only affect the WTO/GATS-compatibility of the RTAs concerned, but contribute to further fragmenting international market conditions. This is particularly unfortunate at a time when regional agreements are the only realistic options to overcome the negotiating stalemate at multilateral level. Hence, given the stakes involved, there is an urgent need for participants to (re-)consider the framework provisions underpinning their RTAs.
The Southern Illinois Twins/Triplets and Siblings Study (SITSS): A Longitudinal Study of Early Child Development
Authors: DiLalla Lisabeth Fisher , Jamnik Matthew R.
Published: July 2019

This article reviews the Southern Illinois Twins/Triplets and Siblings Study (SITSS) and describes some of the findings related to recent projects that were completed using this sample. At this time, the SITSS has enrolled 375 twin pairs, 12 triplet families, 1 family of quadruplets, 98 nontwin sibling pairs and 287 singletons. Testing begins for twins and triplets as young as age 1 and then occurs yearly on their birthdays until 5 years of age. Through age 20, various follow-up studies have been conducted on the SITSS sample to examine their social, emotional, and cognitive development across childhood and adolescence from a behavioral genetic perspective. A variety of methodologies have been used to investigate gene–environment correlations (rGE) and gene–environment interactions (GxE). Advanced statistical procedures (e.g., genetic likelihood indices and multilevel modeling) have been utilized to further investigate genetic underpinnings of behavior. Recent results have indicated genetic influences on the aggressiveness of preschoolers’ media preferences, increased problem behaviors related to young children’s overestimation of self-competence, and the influence of early life temperament and internalizing problems on adolescent health behaviors. Additionally, the SITSS has provided evidence for evocative rGE for various behaviors (aggression, prosocial and play), as well as findings supporting interactions between the dopamine receptor D4 gene (DRD4) and the environment (peer victimization, prenatal birth complications and parental sensitivity). Together, by use of multitrait and multimethodological investigations, this behavior genetic data set assists in furthering our understanding of biological and environmental influences on children’s development.
Gradient‐Based Inverse Estimation for a Rainfall‐Runoff Model
Authors: Krapu Christopher , Borsuk Mark , Kumar Mukesh
Published: July 2019

Recent advances in deep learning for neural networks with large numbers of parameters have been enabled by automatic differentiation, an algorithmic technique for calculating gradients of measures of model fit with respect to model parameters. Estimation of high‐dimensional parameter sets is an important problem within the hydrological sciences. Here, we demonstrate the effectiveness of gradient‐based estimation techniques for high‐dimensional inverse estimation problems using a conceptual rainfall‐runoff model. In particular, we compare the effectiveness of Hamiltonian Monte Carlo and automatic differentiation variational inference against two nongradient‐dependent methods, random walk Metropolis and differential evolution Metropolis. We show that the former two techniques exhibit superior performance for inverse estimation of daily rainfall values and are much more computationally efficient on larger data sets in an experiment with synthetic data. We also present a case study evaluating the effectiveness of automatic differentiation variational inference for inverse estimation over 25 years of daily precipitation conditional on streamflow observations at three catchments and show that it is scalable to very high dimensional parameter spaces. The presented results highlight the power of combining hydrological process‐based models with optimization techniques from deep learning for high‐dimensional estimation problems.
Convergence: A Call for More Robust Global Science Collaboration
Authors: McEntee Chris
Published: July 2019

With nationalism and scientific disinformation on the rise, the scientific community must work together even more closely.
Remote Characterization of Dominant Wavelengths From Surface Folding on Lava Flows Using Lidar and Discrete Fourier Transform Analyses
Authors: Deardorff Nicholas , Booth Adam , Cashman Katharine
Published: July 2019

Surface folding is common in lava flows of all compositions and is believed to be due to changes in viscosity and flow velocity between the cooling crust and the more fluid flow interior. However, our understanding of the relationship between surface folding and flow rheology is incomplete. In this study we analyze digital terrain models of eight lava flows ranging in composition from basaltic andesite to rhyolite using a discrete Fourier transform analysis to quantitatively determine dominant surface fold wavelengths. Our discrete Fourier transform analyses show that each lava flow has multiple fold generations and that dominant wavelengths are more closely related to calculated effective viscosity than to lava composition. At our Oregon sites, average dominant wavelengths generally increase with viscosity (r2=0.68), and the correlation improves (r2=0.87) when expanded by including previously measured fold wavelengths and viscosities from the global database. However, there are a few exceptions to this positive trend where a few lava flows have lower or higher than expected dominant fold wavelengths, which we infer are due to secondary factors such as differences in eruption conditions (eruption rate, temperature, etc.). Additionally, over a 5 order of magnitude range in viscosity, there is significant overlap between the ranges of fold wavelengths, particularly from 10 to 20m, for lavas from basaltic andesite to rhyolite, making it difficult to determine a numeric correlation between surface folds and lava rheology that would allow remote characterization of lava.
Investigating an Unusually Large 28‐Day Oscillation in Mesospheric Temperature Over Antarctica Using Ground‐Based and Satellite Measurements
Authors: Zhao Yucheng , Taylor M. J. , Pautet P.‐D. , Moffat‐Griffin T. , Hervig M. E. , Murphy D. J. , French W. J. R. , Liu H. L. , Pendleton W. R. , Russell J. M.
Published: July 2019

The Utah State University Advanced Mesospheric Temperature Mapper was deployed at the Amundsen‐Scott South Pole Station in 2010 to measure OH temperature at ~87 km as part of an international network to study the mesospheric dynamics over Antarctica. During the austral winter of 2014, an unusually large amplitude ~28‐day oscillation in mesospheric temperature was observed for ~100 days from the South Pole Station. This study investigates the characteristics and global structure of this exceptional planetary‐scale wave event utilizing ground‐based mesospheric OH temperature measurements from two Antarctic stations (South Pole and Rothera) together with satellite temperature measurements from the Microwave Limb Sounder on the Aura satellite and the Solar Occultation For Ice Experiment on the Aeronomy of Ice in the Mesosphere satellite. Our analyses have revealed that this large oscillation is a wintertime, high‐latitude phenomenon, exhibiting a coherent zonal wave #1 structure below 80‐km altitude. At higher altitudes, the wave was confined in longitude between 180°E and 360°E. The amplitude of this oscillation reached ~15 K at 85 km, and it was observed to grow with altitude as it extended from the stratosphere into the lower thermosphere in the Southern Hemisphere. The satellite data further established the existence of this oscillation in the Northern Hemisphere during the boreal wintertime. The main characteristics and global structure of this event as observed in temperature are consistent with the predicted 28‐day Rossby Wave (1,4) mode.
Transcriptome profiling of interaction effects of soybean cyst nematodes and soybean aphids on soybean
Authors: Neupane Surendra , Mathew Febina M. , Varenhorst Adam J. , Nepal Madhav P.
Published: July 2019

Soybean aphid (Aphis glycines; SBA) and soybean cyst nematode (Heterodera glycines; SCN) are two major pests of soybean (Glycine max) in the United States of America. This study aims to characterize three-way interactions among soybean, SBA, and SCN using both demographic and genetic datasets. SCN-resistant and SCN-susceptible soybean cultivars with a combination of soybean aphids (biotype 1) and SCN (HG type 0) in a randomized complete block design (RCBD) with six blocks were used to evaluate the three-way interactions in a greenhouse setup. Treatments receiving SCN were infested at planting with 2000 nematode eggs, and the treatments with soybean aphids were infested at second trifoliate growth stage (V2) with 15 soybean aphids. The whole roots were sampled from plants at 5 and 30 days post SBA infestation for RNA sequencing using Illumina Hiseq. 3000. The data comprises of 47 libraries that are useful for further analyses of important genes, which are involved in interaction effects of SBA and SCN on soybean.
TAF1, associated with intellectual disability in humans, is essential for embryogenesis and regulates neurodevelopmental processes in zebrafish
Authors: Gudmundsson Sanna , Wilbe Maria , Filipek-Górniok Beata , Molin Anna-Maja , Ekvall Sara , Johansson Josefin , Allalou Amin , Gylje Hans , Kalscheuer Vera M. , Ledin Johan , Annerén Göran , Bondeson Marie-Louise
Published: July 2019

The TATA-box binding protein associated factor 1 (TAF1) protein is a key unit of the transcription factor II D complex that serves a vital function during transcription initiation. Variants of TAF1 have been associated with neurodevelopmental disorders, but TAF1’s molecular functions remain elusive. In this study, we present a five-generation family affected with X-linked intellectual disability that co-segregated with a TAF1 c.3568C>T, p.(Arg1190Cys) variant. All affected males presented with intellectual disability and dysmorphic features, while heterozygous females were asymptomatic and had completely skewed X-chromosome inactivation. We investigated the role of TAF1 and its association to neurodevelopment by creating the first complete knockout model of the TAF1 orthologue in zebrafish. A crucial function of human TAF1 during embryogenesis can be inferred from the model, demonstrating that intact taf1 is essential for embryonic development. Transcriptome analysis of taf1 zebrafish knockout revealed enrichment for genes associated with neurodevelopmental processes. In conclusion, we propose that functional TAF1 is essential for embryonic development and specifically neurodevelopmental processes.
Normalization of glycosaminoglycan-derived disaccharides detected by tandem mass spectrometry assay for the diagnosis of mucopolysaccharidosis
Authors: Lin Hsiang-Yu , Lo Yun-Ting , Wang Tuan-Jen , Huang Sung-Fa , Tu Ru-Yi , Chen Tzu-Lin , Lin Shuan-Pei , Chuang Chih-Kuang
Published: July 2019

Mucopolysaccharidosis (MPS) is caused by the deficiency of a specific hydrolytic enzyme that catalyzes the step-wise degradation of glycosaminoglycans (GAGs). In this study, we propose an empirical method to calculate levels of GAG-derived disaccharides based on the quantity (peak areas) of chondroitin sulfate (CS) with the aim of making a diagnosis of MPS more accurate and reducing the occurrence of false positive and false negative results. In this study, levels of urinary GAG-derived disaccharides were measured in 67 patients with different types of MPS and 165 controls without MPS using a tandem mass spectrometry assay. Two different methods of reporting GAG-derived disaccharides were assessed; normalization to urinary CS (in μg/mL), and normalization to μg/mg creatinine. CS-normalization yielded more consistent values than creatinine-normalization. In particular, levels of urinary dermatan sulfate (DS), heparan sulfate (HS), and keratan sulfate (KS) significantly varied because of changes in urine creatinine levels, which were proportional to age but inversely proportional to DS, HS, and KS measurements. Using CS-normalization revealed the actual status of DS, HS, and KS without the influence of factors such as age, urine creatinine, and other physiological conditions. It could discriminate between the patients with MPS and controls without MPS, and also to evaluate changes in GAG levels pre- and post-enzyme replacement therapy.
Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome
Authors: Yanguas Francisco , Moscoso-Romero Esteban , Valdivieso M.-Henar
Published: July 2019

Carboxypeptidases Y (Cpy1) and S (Cps1), the receptor Vps10, and the ATPase subunit Vph1 follow the carboxypeptidase Y (CPY) pathway from the trans-Golgi network (TGN) to the prevacuolar endosome (PVE). Using Schizosaccharomyces pombe quantitative live-cell imaging, biochemical and genetic analyses, we extended the previous knowledge and showed that collaboration between Gga22, the dominant Golgi-localized Gamma-ear-containing ARF-binding (GGA) protein, and Gga21, and between Gga22 and the endosomal epsin Ent3, was required for efficient: i) Vps10 anterograde trafficking from the TGN to the PVE; ii) Vps10 retrograde trafficking from the PVE to the TGN; iii) Cps1 exit from the TGN, and its sorting in the PVE en route to the vacuole; and iv) Syb1/Snc1 recycling to the plasma membrane through the PVE. Therefore, monomeric clathrin adaptors facilitated the trafficking of Vps10 in both directions of the CPY pathway, and facilitated trafficking events of Cps1 in different organelles. By contrast, they were dispensable for Vph1 trafficking. Thus, these adaptors regulated the traffic of some, but not all, of the cargo of the CPY pathway, and regulated the traffic of cargoes that do not follow this pathway. Additionally, this collaboration was required for PVE organization and efficient growth under stress.
Prefrontal cortex neuronal ensembles encoding fear drive fear expression during long-term memory retrieval
Authors: Giannotti Giuseppe , Heinsbroek Jasper A. , Yue Alexander J. , Deisseroth Karl , Peters Jamie
Published: July 2019

The prefrontal cortex is an important regulator of fear expression in humans and rodents. Specifically, the rodent prelimbic (PL) prefrontal cortex drives fear expression during both encoding and retrieval of fear memory. Neuronal ensembles have been proposed to function as memory encoding units, and their re-activation is thought to be necessary for memory retrieval and expression of conditioned behavior. However, it remains unclear whether PL cortex neuronal ensembles that encode fear memory contribute to long-term fear expression during memory retrieval. To address this, we employed a viral-mediated TRAP (Targeted Recombination in Active Population) technology to target PL cortex ensembles active during fear conditioning and expressed the inhibitory Gi-DREADD in fear-encoding ensembles. Male and female rats were trained to lever press for food and subjected to Pavlovian delay fear conditioning, then 28 days later, they underwent a fear memory retrieval test. Chemogenetic inhibition of TRAPed PL cortex ensembles reduced conditioned suppression of food seeking in females, but not males. Neither context nor tone freezing behavior was altered by this manipulation during the same retrieval test. Thus, fear-encoding ensembles in PL cortex drive long-term fear expression in a sex and fear modality dependent manner.
Comparative mitogenomics of the Decapoda reveals evolutionary heterogeneity in architecture and composition
Authors: Tan Mun Hua , Gan Han Ming , Lee Yin Peng , Bracken-Grissom Heather , Chan Tin-Yam , Miller Adam D. , Austin Christopher M.
Published: July 2019

The emergence of cost-effective and rapid sequencing approaches has resulted in an exponential rise in the number of mitogenomes on public databases in recent years, providing greater opportunity for undertaking large-scale comparative genomic and systematic research. Nonetheless, current datasets predominately come from small and disconnected studies on a limited number of related species, introducing sampling biases and impeding research of broad taxonomic relevance. This study contributes 21 crustacean mitogenomes from several under-represented decapod infraorders including Polychelida and Stenopodidea, which are used in combination with 225 mitogenomes available on NCBI to investigate decapod mitogenome diversity and phylogeny. An overview of mitochondrial gene orders (MGOs) reveals a high level of genomic variability within the Decapoda, with a large number of MGOs deviating from the ancestral arthropod ground pattern and unevenly distributed among infraorders. Despite the substantial morphological and ecological variation among decapods, there was limited evidence for correlations between gene rearrangement events and species ecology or lineage specific nucleotide substitution rates. Within a phylogenetic context, predicted scenarios of rearrangements show some MGOs to be informative synapomorphies for some taxonomic groups providing strong independent support for phylogenetic relationships. Additional comparisons for a range of mitogenomic features including nucleotide composition, strand asymmetry, unassigned regions and codon usage indicate several clade-specific trends that are of evolutionary and ecological interest.
The MmpL3 interactome reveals a complex crosstalk between cell envelope biosynthesis and cell elongation and division in mycobacteria
Authors: Belardinelli Juan Manuel , Stevens Casey M. , Li Wei , Tan Yong Zi , Jones Victoria , Mancia Filippo , Zgurskaya Helen I. , Jackson Mary
Published: July 2019

Integral membrane transporters of the Mycobacterial Membrane Protein Large (MmpL) family and their interactome play important roles in the synthesis and export of mycobacterial outer membrane lipids. Despite the current interest in the mycolic acid transporter, MmpL3, from the perspective of drug discovery, the nature and biological significance of its interactome remain largely unknown. We here report on a genome-wide screening by two-hybrid system for MmpL3 binding partners. While a surprisingly low number of proteins involved in mycolic acid biosynthesis was found to interact with MmpL3, numerous enzymes and transporters participating in the biogenesis of peptidoglycan, arabinogalactan and lipoglycans, and the cell division regulatory protein, CrgA, were identified among the hits. Surface plasmon resonance and co-immunoprecipitation independently confirmed physical interactions for three proteinsin vitroand/orin vivo. Results are in line with the focal localization of MmpL3 at the poles and septum of actively-growing bacilli where the synthesis of all major constituents of the cell wall core are known to occur, and are further suggestive of a role for MmpL3 in the coordination of new cell wall deposition during cell septation and elongation. This novel aspect of the physiology of MmpL3 may contribute to the extreme vulnerability and high therapeutic potential of this transporter.
Carbon dots with molecular fluorescence and their application as a “turn-off” fluorescent probe for ferricyanide detection
Authors: Wang Tianshu , Wang Ailin , Wang Ruixue , Liu Zhaoyang , Sun Ying , Shan Guiye , Chen Yanwei , Liu Yichun
Published: July 2019

Highly fluorescent carbon dots (CDs) exhibiting molecular fluorescence were synthesized and successfully used for sensing ferricyanide based on fluorescence quenching. We conducted dialysis to purify the CDs and found that the dialysate is also fluorescent. From the mass spectra and quantum yield analyses of the dialysate, it is demonstrated that molecular fluorophores were also synthesized during the synthesis of CDs. By the comparison of fluorescence spectra between CDs and dialysate, it is established that the fluorescence emission of CDs partly originates from fluorophores that are attached to CDs’ surface. The fluorescence quenching caused by ferricyanide is proved to be the overlap of absorption spectra between ferricyanide and CDs. The changes of the absorbance and fluorescence spectra are combined to enhance the detection sensitivity, and the limit of detection is calculated to be 1.7 μM. A good linear response of fluorescence-absorbance combined sensing toward ferricyanide is achieved in the range of 5–100 µM. This method is highly selective to ferricyanide among other common cations and anions, and it is also successfully applied in detecting ferricyanide in real water samples.
Precise and systematic survey of the efficacy of multicomponent drugs against functional dyspepsia
Authors: Wei Junying , Man Qiong , Guo Feifei , Xian Minghua , Wang Tingting , Tang Chunyu , Zhang Yi , Li Defeng , Tang Daifeng , Yang Hongjun , Huang Luqi
Published: July 2019

Functional dyspepsia (FD) is one of the most prevalent functional gastrointestinal disorders, and more and more multicomponent drugs represented by traditional Chinese medicines have provided a favorable therapeutic effect in its treatment. However, their precise localization in the clinic, as well as corresponding mechanism, is ambiguous, thus hindering their widespread use. To meet this requirement, a precise and systematic approach based on a restriction of special disease-related molecules and the following network pharmacology analysis was developed and applied to a multicomponent conventional drug, XiaoErFuPi (XEFP) granules. Experimental verification of the results indicates that this approach can facilitate the prediction, and the precise and systematic efficacy of XEFP could be easily revealed, which shows that XEFP has an advantage over the positive control drug on lactate, gastrin, interleukin 4 and calcitonin gene-related peptide. Moreover, by the proteomics analysis, its superposition of multi-target effects was revealed and a new candidate target for the treatment of FD, striatin, was obtained and verified. This study provides a practicable precise approach for the investigation of the efficacy of multicomponent drugs against FD and offers a promising alternative for the systematical management of FD.