bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Amino Acids in Pigs and Chickens: Implication for Gut Health
Authors: Chalvon-Demersay Tristan , Luise Diana , Le Floc'h Nathalie , Tesseraud Sophie , Lambert William , Bosi Paolo , Trevisi Paolo , Beaumont Martin , Corrent Etienne
Published: May 2021

In pigs and broiler chickens, the gastrointestinal tract or gut is subjected to many challenges which alter performance, animal health, welfare and livability. Preventive strategies are needed to mitigate the impacts of these challenges on gut health while reducing the need to use antimicrobials. In the first part of the review, we propose a common definition of gut health for pig and chickens relying on four pillars, which correspond to the main functions of the digestive tract: (i) epithelial barrier and digestion, (ii) immune fitness, (iii) microbiota balance and (iv) oxidative stress homeostasis. For each pillar, we describe the most commonly associated indicators. In the second part of the review, we present the potential of functional amino acid supplementation to preserve and improve gut health in piglets and chickens. We highlight that amino acid supplementation strategies, based on their roles as precursors of energy and functional molecules, as signaling molecules and as microbiota modulators can positively contribute to gut health by supporting or restoring its four intertwined pillars. Additional work is still needed in order to determine the effective dose of supplementation and mode of administration that ensure the full benefits of amino acids. For this purpose, synergy between amino acids, effects of amino acid-derived metabolites and differences in the metabolic fate between free and protein-bound amino acids are research topics that need to be furtherly investigated.
Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus
Authors: Yang Dahan , Lv Xiaoping , Zhang Shujun , Zheng Shimin
Published: May 2021

Reticuloendotheliosis virus (REV) is a type C avian retrovirus that causes immunosuppression, dwarf syndrome, and lymphoma in infected hosts. In this study, we used tandem mass tag (TMT) labeling and liquid chromatography–tandem mass spectrometry (LC-MS/MS) to characterize protein alterations in chicken bursa of Fabricius, before and after REV infection at 7, 14, 21, and 28 days. Our data showed that 1,127, 999, 910, and 1,138 differentially expressed proteins were significantly altered at 7, 14, 21, and 28 days after REV infection, respectively. Morphological analysis showed that REV infection reduced in cortical lymphocytes, bursal follicle atrophy, and nuclear damage. Bioinformatics analysis indicated these proteins were mainly involved with immune responses, energy metabolism, cellular processes, biological regulation, metabolic processes, response to stimuli, and multicellular organismal process. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analysis showed that post-infection, proteins were enriched in the cell cycle, Wnt signaling, antigen processing and presentation, cytokine receptor interaction, adenosine 3′,5′-cyclic monophosphate signaling pathway, and NF-κB signaling. In addition, we observed that peroxiredoxin 4 (PRDX4), peroxiredoxin 6 (PRDX6), glutathione peroxidase 3 (GPX3), catalase (CAT), and peroxidasin (PXDN) were involved in oxidative stress. Some heat shock protein (HSP) family members such as HSPH1, DNAJA4, HSPA8, and HSPA4L also changed significantly after REV infection. These findings help clarify interactions between REV and the host and provides mechanistic insights on REV-induced host immunosuppression.
Family Firms and Innovation from Founder to Successor
Authors: Cesaroni Francesca Maria , Chamochumbi Diaz Gail Denisse , Sentuti Annalisa
Published: May 2021

Several empirical investigations indicate that family firms are more innovative under the founding generation’s leadership and become less innovative in later stages, while others state the opposite. Within this debate, limited attention has been devoted to understanding how intra-family succession might be an opportunity to maintain or improve family firms’ innovativeness. This paper aims to explore how family firms’ innovativeness may evolve from the first to the second generation and understand which conditions may favour or hamper this change. A qualitative approach based on a multiple case study was adopted, conducting seven face-to-face semi-structured interviews with founders and successors that formed the basis of four case studies. The results reveal four different dynamics that characterise how a first-generation family firm’s innovation capacities are or are not passed on to the second generation: decline, transformation, consolidation and preservation. Findings also show that these dynamics depend on the founders and successors’ approaches towards innovation. To better depict differences between them, we propose a typology of founders (lone innovator, collaborative innovator and orchestrator innovator) and successors (prodigal son, game changer, talent scout, faithful disciple) and explain how they influence the evolution of innovation from the founder generation to the next.
Study on Film Cooling Performance of Round Hole Embedded in Different Shaped Craters and Trenches
Authors: Wu Xiaojun , Du Xin , Wang Chunhua
Published: May 2021

Film cooling effectiveness can be improved significantly by embedding a round hole in trenches or craters. In this study, film cooling performances of a transverse trench, W-shaped trench and elliptic trench were compared and analyzed in detail. The CFD models for trench film cooling were established and validated via the experimental results. Inside the transverse trench, a pair of recirculating vortices is formed, which promotes the coolant spreading in a lateral direction. The decrease of trench width and increase of trench depth both improve the film cooling effectiveness of the transverse trench. For the W-shaped trench, the guide effect of the corner angle further improves the lateral spreading capability of coolant and generates higher cooling effectiveness than a transverse trench with the same depth and width. The flow characteristics of the elliptic trench are similar to that of the round hole, and the kidney vortex pair takes a dominant role in the flow fields downstream of the coolant exit. Accordingly, the elliptic trench generates the worst cooling performance in these shaped trenches. The increase of trench depth and decrease of trench width both result in an increase of the discharge coefficient for trench film cooling. For the W-shaped trench, the increase of the corner angle causes a decrease of the discharge coefficient. For the elliptic trench, the discharge coefficient increases with the decrease of the elliptic aspect ratio (major axis/minor axis).
Screening of Functional Compounds in Supercritical Carbon Dioxide Extracts from Perennial Herbaceous Crops
Authors: Ostolski Mateusz , Adamczak Marek , Brzozowski Bartosz , Stolarski Mariusz Jerzy
Published: May 2021

The bio-based economy concept requires using biomass not only for energy production but also for bioactive compound extraction, application or biotransformation. This study analyzed the possibility of obtaining bioactive compounds from biomass before its transformation into biofuel. This involved an analysis of the total content of polyphenols (TPC), flavonoids (TFC), and spectral analysis using Fourier transform infrared spectroscopy (QATR- FTIR) as well as analysis of the antioxidant activity of extracts from selected perennial herbaceous crops cultivated on marginal lands in Poland. The extracts were obtained by supercritical carbon dioxide extraction (scCO2) or scCO2 with water as a cosolvent (scCO2/H2O) from biomass of the following plants: Helianthus salicifolius, Silphium perfoliatum, Helianthus tuberosus, Miscanthus × giganteus, Miscanthus sacchariflorus, Miscanthus sinensis and Spartina pectinata. The biomass was harvested twice during the growing period (June and October) and once after the end of the growing period (February). For most of the analyzed extracts obtained from biomass at the growing stage using scCO2 or scCO2/H2O, a higher TPC was noted than for samples of semi-wood or straw biomass obtained after the end of the growing period. Higher contents of polyphenolic compounds were recorded in extracts obtained using scCO2/H2O. A positive correlation between TPC and antioxidant activity was noted for the analyzed substrates. Flavonoid contents varied in the analyzed samples, and higher contents were generally obtained in scCO2 extracts from biomass harvested at the beginning of the growing period. A high diversity of extract compositions was confirmed by spectral analysis. The presented data can be used at the initial stage of planning a biorefinery.
Quantification of Total Phenols, Tannins, Anthocyanins Content in Myrtus communis L. and Antioxidant Activity Evaluation in Function of Plant Development Stages and Altitude of Origin Site
Authors: Medda Silvia , Fadda Angela , Dessena Leonarda , Mulas Maurizio
Published: May 2021

Most of the biological properties of myrtle (Myrtus communis L.) are linked to the antioxidant activity of the phenolic compounds present in the extracts. In this study, the content of total phenols, tannins, and anthocyanins of acidified ethanol extracts of berries and leaves of five myrtle cultivars was quantified during the whole fruit development. In some development stages, the antioxidant activity (AA) of both tissues was evaluated with FRAP, ABTS, DPPH, β-carotene assays, and spectroscopic method (EPR). The trend of AA in leaf and berry extracts was different among genotypes and evaluation assays. In general, leaves showed with DPPH assay, the highest AA during autumn season, while with FRAP and β-carotene was higher at 120 DAF (days after flowering) corresponding to summer season. In berries, the AA with DPPH, ABTS, and EPR assays was the highest for all cultivars at 150 DAF and 210 DAF, while with β-carotene assay was higher in the first development stage. Both in leaf and berry, the total phenols and tannins contents influence the AA depending on the assay used, while the berries anthocyanins seem to play a minor role. The altitude of the site of origin differently affected the AA of myrtle leaf and berry. The study showed the potentiality to use the studied myrtle genotypes for further evaluation on biological applications of antioxidant activity.
Effect of Naturally Occurring Compounds on Fumonisin Production and fum Gene Expression in Fusarium verticillioides
Authors: Ferrigo Davide , Bharti Sharda , Mondin Massimiliano , Raiola Alessandro
Published: May 2021

Fusarium verticillioides, one of the most common pathogens in maize, is responsible for yield losses and reduced kernel quality due to contamination by fumonisins (FBs). Two F. verticillioides isolates that differed in their ability to produce FBs were treated with a selection of eight natural phenolic compounds with the aim of identifying those that were able to decrease toxin production at concentrations that had a limited effect on fungal growth. Among the tested compounds, ellagic acid and isoeugenol, which turned out to be the most effective molecules against fungal growth, were assayed at lower concentrations, while the first retained its ability to inhibit toxin production in vitro, the latter improved both the fungal growth and FB accumulation. The effect of the most effective phenolic compounds on FB accumulation was also tested on maize kernels to highlight the importance of appropriate dosages in order to avoid conditions that are able to promote mycotoxin biosynthesis. An expression analysis of genes involved in FB production allowed more detailed insights into the mechanisms underlying the inhibition of FBs by phenolic compounds. The expression of the fum gene was generally down-regulated by the treatments; however, some treatments in the low-producing F. verticillioides strain up-regulated fum gene expression without improving FB production. This study showed that although different phenolic compounds are effective for FB reduction, they can modulate biosynthesis at the transcription level in opposite manners depending on strain. In conclusion, on the basis of in vitro and in vivo screening, two out of the eight tested phenols (ellagic acid and carvacrol) appear to be promising alternative molecules for the control of FB occurrence in maize.
Nitrogen Source Influences the Effect of Plant Growth-Promoting Rhizobacteria (PGPR) on Macadamia integrifolia
Authors: Gallart Marta , Paungfoo-Lonhienne Chanyarat , Gonzalez Axayacatl , Trueman Stephen J.
Published: May 2021

The potential of plant growth-promoting rhizobacteria (PGPR) to improve plant growth and nutrient acquisition has received increased attention. This study investigated the synergistic effects of combining PGPR Paraburkholderia sp. SOS3 with the addition of inorganic N fertilizer (iN) or a combined application of inorganic N with organic manure-based fertilizer (iNoN) at low and high levels of N fertilization on Macadamia integrifolia seedling growth. We studied plant growth, mineral N in soil-leachate, and media physicochemical and microbial characteristics. Growth of seedlings fertilized with iNoN at high N level (iNoN 100) was enhanced by PGPR inoculation, with an increase of 32% in total biomass and 43% in N uptake, compared with uninoculated seedlings. No significant PGPR effect was observed on growth under low or high inorganic N treatments but PGPR significantly reduced N leaching after 3 weeks of fertilization. We found a positive relationship between media and plant δ15N and plant N uptake, and a strong increase in microbial-biomass N under the most productive fertilization treatment (iNoN 100 with PGPR), compared with the other N treatments (without or with PGPR). The results suggest that PGPR improves N acquisition by reducing mineral N loss and increasing plant N availability, but that these effects depend on the N form and N level.
Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle
Authors: Li Liang , Deng Xun , Hu Silu , Cui Zhifu , Ning Zifan , Gui Taotao , Zhao Xiaoling , Li Diyan , Wang Yan , Yin Huadong , Ye Lin , Tian Yaofu , Zhang Yao , Li Hua , Zhu Qing
Published: May 2021

Long non-coding RNAs (lncRNAs) and mRNAs are temporally expressed during chicken follicle development. However, follicle transcriptome studies in chickens with timepoints relating to changes in luteinizing hormone (LH) levels are rare. In this study, gene expression in Rohman layers was investigated at three distinct stages of the ovulatory cycle: zeitgeber time 0 (ZT0, 9:00 a.m.), zeitgeber time 12 (ZT12, 9:00 p.m.), and zeitgeber time 20 (ZT20, 5:00 a.m.) representing the early, middle, and LH surge stages, respectively, of the ovulatory cycle. Gene expression profiles were explored during follicle development at ZT0, ZT12, and ZT20 using Ribo-Zero RNA sequencing. The three stages were separated into two major stages, including the pre-LH surge and the LH surge stages. A total of 12,479 mRNAs and 7528 lncRNAs were identified among the three stages, and 4531, 523 differentially expressed genes (DEGs) and 2367, 211 differentially expressed lncRNAs (DELs) were identified in the ZT20 vs. ZT12, and ZT12 vs. ZT0, comparisons. Functional enrichment analysis revealed that genes involved in cell proliferation and metabolism processes (lipid-related) were mainly enriched in the ZT0 and ZT12 stages, respectively, and genes related to oxidative stress, steroids regulation, and inflammatory process were enriched in the ZT20 stage. These findings provide the basis for further investigation of the specific genetic and molecular functions of follicle development in chickens.
Successful Use of Geochemical Tools to Trace the Geographic Origin of Long-Snouted Seahorse Hippocampus guttulatus Raised in Captivity
Authors: Cabral Ana Elisa , Ricardo Fernando , Patinha Carla , Silva Eduardo Ferreira da , Correia Miguel , Palma Jorge , Planas Miquel , Calado Ricardo
Published: May 2021

The global market of dried seahorses mainly supplies Traditional Chinese Medicine and still relies on blurry trade chains that often cover less sustainable practices targeting these pricey and endangered fish. As such, reliable tools that allow the enforcement of traceability, namely to confirm the geographic origin of traded seahorses, are urgently needed. The present study evaluated the use of elemental fingerprints (EF) in the bony structures of long-snouted seahorses Hippocampus guttulatus raised in captivity in two different locations (southern Portugal and Northern Spain) to discriminate their geographic origin. The EF of different body parts of H. guttulatus were also evaluated as potential proxies for the EF of the whole body, in order to allow the analysis of damaged specimens and avoid the use of whole specimens for analysis. The contrasting EF of H. guttulatus raised in the two locations allowed their reliable discrimination. Although no single body part exactly mimicked the EF of the whole body, seahorse trunks, as well as damaged specimens, could still be correctly allocated to their geographic origin. This promising forensic approach to discriminate the geographic origin of seahorses raised in captivity should now be validated for wild conspecifics originating from different locations, as well as for other species within genus Hippocampus.
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish
Authors: Orso Graziella , Solovyev Mikhail M. , Facchiano Serena , Tyrikova Evgeniia , Sateriale Daniela , Kashinskaya Elena , Pagliarulo Caterina , Hoseinifar Hossein S. , Simonov Evgeniy , Varricchio Ettore , Paolucci Marina , Imperatore Roberta
Published: May 2021

The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (Castanea sativa) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (Danio rerio) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (Enterobacteriaceae and Pseudomonas), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields.
Aquaporins Are Differentially Regulated in Canine Cryptorchid Efferent Ductules and Epididymis
Authors: Squillacioti Caterina , Mirabella Nicola , Liguori Giovanna , Germano Giuseppe , Pelagalli Alessandra
Published: May 2021

The efferent ductules and the epididymis are parts of the male reproductive system where spermatozoa mature. Specialized epithelial cells in these ducts contribute to the transport of fluids produced by spermatozoa’s metabolic activity. Aquaporins (AQPs) have been demonstrated to be expressed in the spermatozoan membrane and testis epithelial cells, where they contribute to regulating spermatozoan volume and transit through environments of differing osmolality. Due to the lack of detailed literature regarding AQP expression in the canine male genital tract, the aim of this study was to investigate both the distribution and expression of AQP7, AQP8, and AQP9 in the efferent ductules and epididymal regions (caput, corpus, and cauda) from normal and cryptorchid dogs by using immunohistochemistry, Western blotting, and real-time reverse transcription polymerase chain reaction (RT-PCR). Our results show different patterns for the distribution and expression of the examined AQPs, with particular evidence of their upregulation in the caput and downregulation in the cauda region of the canine cryptorchid epididymis. These findings are associated with a modulation of Hsp70 and caspase-3 expression, suggesting the participation of AQPs in the luminal microenvironment modifications that are peculiar characteristics of this pathophysiological condition.
The Root towards More Circularized Animal Production Systems: From Animal to Territorial Metabolism
Authors: De Rosa Marcello , Di Pasquale Jorgelina , Adinolfi Felice
Published: May 2021

This paper deals with a relevant topic in the literature on sustainable management of animal farms, concerning the transition towards circular methods of animal production. The paper aims to put forward an original analytical multilevel perspective overlapping different dimensions at either micro, meso, and macro level. Starting from the Malthusian analysis on depletion of natural resources, with risks of the fragility of the natural and economic systems, the paper points out the importance of moving away from intensive methods of production, by adopting more circularized approaches based on resources efficiency. The application of circular economy approaches to animal production is theorized through the concept of territorial metabolism involving not only internal resources (at the animal farm level) but also territorial resources. The paper underlines the critical points of the transition, which is labeled as a socio-technical transition in that it involves not only technical issues but also social aspects. Critical points are addressed through consumers’ acceptance of products drawn on circular approaches and political support to transition, through political tools which are boosted in recent documents of the European Union, like the Green Deal and Farm to Fork strategy.
Selected Alternative Feed Additives Used to Manipulate the Rumen Microbiome
Authors: Michalak Marta , Wojnarowski Konrad , Cholewińska Paulina , Szeligowska Natalia , Bawej Marcel , Pacoń Jakub
Published: May 2021

In recent years, a boost in the ruminant population has been observed, and consequently, an increase in the animals’ demand for nutrients and methane emissions. Methane emission is generated during the microbial fermentation of feed in the rumen, and a percentage even up to 12% of the energy obtained by this process can be wasted. In addition, the use of antibiotics in animal husbandry is being increasingly restricted. restricted. As a result, there is a continuous search for innovative feed additives that can serve as alternatives to antibiotics, and will also be safe for both people and the environment. In the present review article, additives were selected on basis that, according to studies conducted so far, may positively affect the microbiome of the digestive system by improving indicators and/or reducing methane production. Among them, probiotics, prebiotics or their combination—synbiotics are at the forefront of research. However, additives in the form of algae or plant origin are also gaining ground in popularity, such as essential oils, fermented wheat straw or Gelidium amansii, due to their general recognition as safe (GRAS) for both humans and environment.
Keel Bone Damage in Laying Hens—Its Relation to Bone Mineral Density, Body Growth Rate and Laying Performance
Authors: Habig Christin , Henning Martina , Baulain Ulrich , Jansen Simon , Scholz Armin Manfred , Weigend Steffen
Published: May 2021

Keel bone damage is an important animal welfare problem in laying hens. Two generations of four layer lines, differing in phylogenetic background and performance level and kept in single cages or floor pens were weighed and scored for keel bone deformities (KBD) during the laying period. KBD, keel bone fractures (KBF) and the bone mineral density (BMD) of the keels were assessed post mortem. For BMD, relationships to laying performance and body growth were estimated. Caged hens showed more deformities, but fewer fractures and a lower BMD of the keel bone than floor-housed hens. White-egg layers had a lower BMD (0.140–0.165 g/cm2) and more KBD than brown-egg layers (0.179–0.184 g/cm2). KBF occurred more often in the high-performing lines than the moderate-performing ones. However, in the high-performing lines, BMD was positively related to total egg number from 18 to 29 weeks of age. The adult body weight derived from fitted growth curves (Gompertz function) had a significant effect (p < 0.001) on keels’ BMD. The study contributes to the understanding of predisposing factors for keel bone damage in laying hens. It showed that the growth rate has a rather subordinate effect on keels’ BMD, while the BMD itself greatly affects KBD.
Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid
Authors: Kamiya Sato , Shimizu Kaori , Okada Ayaka , Inoshima Yasuo
Published: May 2021

In this study, to establish whether serum amyloid A (SAA) 3 plays a role in the defense against bacterial infection in mouse mammary epithelium, normal murine mammary gland (NMuMG) epithelial cells were stimulated with lipopolysaccharide (LPS) and lipoteichoic acid (LTA). LPS and LTA significantly enhanced mRNA expression level of the Saa3 gene, whereas no significant change was observed in the Saa1 mRNA level. Furthermore, LPS induced SAA3 protein expression more strongly than LTA, whereas neither LPS nor LTA significantly affected SAA1 protein expression. These data indicate that the expression of SAA3 in mouse mammary epithelial cells was increased by the stimulation with bacterial antigens. SAA3 has been reported to stimulate neutrophils in the intestinal epithelium and increase interleukin-22 expression, which induces activation of the innate immune system and production of antibacterial proteins, such as antimicrobial peptides. Therefore, collectively, these data suggest that SAA3 is involved in the defense against bacterial infection in mouse mammary epithelium.
Deep Learning Classification of Canine Behavior Using a Single Collar-Mounted Accelerometer: Real-World Validation
Authors: Chambers Robert D. , Yoder Nathanael C. , Carson Aletha B. , Junge Christian , Allen David E. , Prescott Laura M. , Bradley Sophie , Wymore Garrett , Lloyd Kevin , Lyle Scott
Published: May 2021

Collar-mounted canine activity monitors can use accelerometer data to estimate dog activity levels, step counts, and distance traveled. With recent advances in machine learning and embedded computing, much more nuanced and accurate behavior classification has become possible, giving these affordable consumer devices the potential to improve the efficiency and effectiveness of pet healthcare. Here, we describe a novel deep learning algorithm that classifies dog behavior at sub-second resolution using commercial pet activity monitors. We built machine learning training databases from more than 5000 videos of more than 2500 dogs and ran the algorithms in production on more than 11 million days of device data. We then surveyed project participants representing 10,550 dogs, which provided 163,110 event responses to validate real-world detection of eating and drinking behavior. The resultant algorithm displayed a sensitivity and specificity for detecting drinking behavior (0.949 and 0.999, respectively) and eating behavior (0.988, 0.983). We also demonstrated detection of licking (0.772, 0.990), petting (0.305, 0.991), rubbing (0.729, 0.996), scratching (0.870, 0.997), and sniffing (0.610, 0.968). We show that the devices’ position on the collar had no measurable impact on performance. In production, users reported a true positive rate of 95.3% for eating (among 1514 users), and of 94.9% for drinking (among 1491 users). The study demonstrates the accurate detection of important health-related canine behaviors using a collar-mounted accelerometer. We trained and validated our algorithms on a large and realistic training dataset, and we assessed and confirmed accuracy in production via user validation.
Development of Phenalenone-Triazolium Salt Derivatives for aPDT: Synthesis and Antibacterial Screening
Authors: Godard Jérémy , Gibbons Dáire , Leroy-Lhez Stéphanie , Williams René M. , Villandier Nicolas , Ouk Tan-Sothéa , Brégier Frédérique , Sol Vincent
Published: May 2021

The increasing number of hospital-acquired infections demand the development of innovative antimicrobial treatments. Antimicrobial photodynamic therapy (aPDT) is a versatile technique which relies on the production of reactive oxygen species (ROS) generated by light-irradiated photosensitizers (PS) in the presence of oxygen (O2). 1H-Phenalen-1-one is a very efficient photosensitizer known for its high singlet oxygen quantum yield and its antimicrobial potential in aPDT when covalently bound to quaternary ammonium groups. Triazolium salts are stable aromatic quaternary ammonium salts that recently appeared as interesting moieties endowed with antimicrobial activities. The coupling between phenalenone and triazolium groups bearing various substituents was realized by copper-catalyzed azide-alkyne cycloaddition followed by alkylation with methyl iodide or 2-(bromomethyl)-1H-phenalen-1-one. As expected, most of the compounds retained the initial singlet oxygen quantum yield, close to unity. Minimum inhibitory concentrations (MIC) of 14 new phenalenone-triazolium salt derivatives and 2 phenalenone-triazole derivatives were determined against 6 bacterial strains (Gram-negatives and Gram-positives species). Most of these PS showed significant photoinactivation activities, the strongest effects being observed against Gram-positive strains with as low as submicromolar MIC values.
In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli
Authors: Svanberg Frisinger Frida , Jana Bimal , Donadio Stefano , Guardabassi Luca
Published: May 2021

Novel antimicrobials interfering with pathogen-specific targets can minimize the risk of perturbations of the gut microbiota (dysbiosis) during therapy. We employed an in silico approach to identify essential proteins in Escherichia coli that are either absent or have low sequence identity in seven beneficial taxa of the gut microbiota: Faecalibacterium, Prevotella, Ruminococcus, Bacteroides, Lactobacillus, Lachnospiraceae and Bifidobacterium. We identified 36 essential proteins that are present in hyper-virulent E. coli ST131 and have low similarity (bitscore < 50 or identity < 30% and alignment length < 25%) to proteins in mammalian hosts and beneficial taxa. Of these, 35 are also present in Klebsiella pneumoniae. None of the proteins are targets of clinically used antibiotics, and 3D structure is available for 23 of them. Four proteins (LptD, LptE, LolB and BamD) are easily accessible as drug targets due to their location in the outer membrane, especially LptD, which contains extracellular domains. Our results indicate that it may be possible to selectively interfere with essential biological processes in Enterobacteriaceae that are absent or mediated by unrelated proteins in beneficial taxa residing in the gut. The identified targets can be used to discover antimicrobial drugs effective against these opportunistic pathogens with a decreased risk of causing dysbiosis.
Mitochondrial Succinate Metabolism and Reactive Oxygen Species Are Important but Not Essential for Eliciting Carotid Body and Ventilatory Responses to Hypoxia in the Rat
Authors: Swiderska Agnieszka , Coney Andrew M. , Alzahrani Abdulaziz A. , Aldossary Hayyaf S. , Batis Nikolaos , Ray Clare J. , Kumar Prem , Holmes Andrew P.
Published: May 2021

Reflex increases in breathing in response to acute hypoxia are dependent on activation of the carotid body (CB)—A specialised peripheral chemoreceptor. Central to CB O2-sensing is their unique mitochondria but the link between mitochondrial inhibition and cellular stimulation is unresolved. The objective of this study was to evaluate if ex vivo intact CB nerve activity and in vivo whole body ventilatory responses to hypoxia were modified by alterations in succinate metabolism and mitochondrial ROS (mitoROS) generation in the rat. Application of diethyl succinate (DESucc) caused concentration-dependent increases in chemoafferent frequency measuring approximately 10–30% of that induced by severe hypoxia. Inhibition of mitochondrial succinate metabolism by dimethyl malonate (DMM) evoked basal excitation and attenuated the rise in chemoafferent activity in hypoxia. However, approximately 50% of the response to hypoxia was preserved. MitoTEMPO (MitoT) and 10-(6′-plastoquinonyl) decyltriphenylphosphonium (SKQ1) (mitochondrial antioxidants) decreased chemoafferent activity in hypoxia by approximately 20–50%. In awake animals, MitoT and SKQ1 attenuated the rise in respiratory frequency during hypoxia, and SKQ1 also significantly blunted the overall hypoxic ventilatory response (HVR) by approximately 20%. Thus, whilst the data support a role for succinate and mitoROS in CB and whole body O2-sensing in the rat, they are not the sole mediators. Treatment of the CB with mitochondrial selective antioxidants may offer a new approach for treating CB-related cardiovascular–respiratory disorders.
Redox Regulation of the Quorum-sensing Transcription Factor AgrA by Coenzyme A
Authors: Baković Jovana , Yu Bess Yi Kun , Silva Daniel , Baczynska Maria , Peak-Chew Sew Yeu , Switzer Amy , Burchell Lynn , Wigneshweraraj Sivaramesh , Vandanashree Muralidharan , Gopal Balasubramanian , Filonenko Valeriy , Skehel Mark , Gout Ivan
Published: May 2021

Staphylococcus aureus (S. aureus) is an aggressive opportunistic pathogen of prominent virulence and antibiotic resistance. These characteristics are due in part to the accessory gene regulator (agr) quorum-sensing system, which allows for the rapid adaptation of S. aureus to environmental changes and thus promotes virulence and the development of pathogenesis. AgrA is the agr system response regulator that binds to the P2 and P3 promoters and upregulates agr expression. In this study, we reveal that S. aureus AgrA is modified by covalent binding of CoA (CoAlation) in response to oxidative or metabolic stress. The sites of CoAlation were mapped by liquid chromatography tandem mass spectrometry (LC–MS/MS) and revealed that oxidation-sensing Cys199 is modified by CoA. Surface plasmon resonance (SPR) analysis showed an inhibitory effect of CoAlation on the DNA-binding activity, as CoAlated AgrA had significantly lower affinity towards the P2 and P3 promoters than non-CoAlated AgrA. Overall, this study provides novel insights into the mode of transcriptional regulation in S. aureus and further elucidates the link between the quorum-sensing and oxidation-sensing roles of the agr system.
Protein Engineering of Electron Transfer Components from Electroactive Geobacter Bacteria
Authors: Fernandes Tomás M. , Morgado Leonor , Turner David L. , Salgueiro Carlos A.
Published: May 2021

Electrogenic microorganisms possess unique redox biological features, being capable of transferring electrons to the cell exterior and converting highly toxic compounds into nonhazardous forms. These microorganisms have led to the development of Microbial Electrochemical Technologies (METs), which include applications in the fields of bioremediation and bioenergy production. The optimization of these technologies involves efforts from several different disciplines, ranging from microbiology to materials science. Geobacter bacteria have served as a model for understanding the mechanisms underlying the phenomenon of extracellular electron transfer, which is highly dependent on a multitude of multiheme cytochromes (MCs). MCs are, therefore, logical targets for rational protein engineering to improve the extracellular electron transfer rates of these bacteria. However, the presence of several heme groups complicates the detailed redox characterization of MCs. In this Review, the main characteristics of electroactive Geobacter bacteria, their potential to develop microbial electrochemical technologies and the main features of MCs are initially highlighted. This is followed by a detailed description of the current methodologies that assist the characterization of the functional redox networks in MCs. Finally, it is discussed how this information can be explored to design optimal Geobacter-mutated strains with improved capabilities in METs.
Inflammation and Oxidative Damage in Ischaemic Renal Disease
Authors: de Bhailís Áine M. , Chrysochou Constantina , Kalra Philip A.
Published: May 2021

Ischaemic renal disease as result of atherosclerotic renovascular disease activates a complex biological response that ultimately leads to fibrosis and chronic kidney disease. Large randomised control trials have shown that renal revascularisation in patients with atherosclerotic renal artery disease does not confer any additional benefit to medical therapy alone. This is likely related to the activation of complex pathways of oxidative stress, inflammatory cytokines and fibrosis due to atherosclerotic disease and hypoxic injury due to reduced renal blood flow. New evidence from pre-clinical trials now indicates a role for specific targeted therapeutic interventions to counteract this complex pathogenesis. This evidence now suggests that the focus for those with atherosclerotic renovascular disease should be a combination of revascularisation and renoprotective therapies that target the renal tissue response to ischaemia, reduce the inflammatory infiltrate and prevent or reduce the fibrosis.
Antimigration Effects of the Number of Flaps on a Plastic Stent: Three-Dimensionally Printed Pancreatic Phantom and Ex Vivo Studies
Authors: Ryu Dae-Sung , Choi Joon-Myeong , Kim Woo-Jin , Kang Jeon-Min , Lee Ki-Baek , Park Yu-Been , Won Dong-Sung , Kim Ji-Won , Kim Song-Hee , Kim Pyeong-Hwa , Kim Song-Cheol , Park Jung-Hoon
Published: May 2021

Stent migration is a significant obstacle to successful stent placement. There has been no investigation of the effect and quantitative interpretation of flaps attached to a plastic stent (PS) on antimigration. The antimigration effects of the number of flaps on a PS in a 3D-printed pancreatic phantom (3DP) and extracted porcine pancreas (EPP) were investigated. Four PS types were used in this study: stent without flaps (type 1), stent with two flaps (type 2), stent with four horizontally made flaps (type 3), and stent with four vertically made flaps (type 4). The stents were measured and compared for antimigration force (AMF) in the 3DP and EPP using a customized measuring method and an integrated measuring device. The mean maximum AMFs (MAMFs) in types 2, 3, and 4 were significantly higher than that in type 1 (all p < 0.001). Moreover, the mean MAMFs in types 3 and 4 were significantly higher than that in type 2 (all p < 0.001). When the flaps were removed from the pancreatic duct, the AMF decreased rapidly. As the number of flaps increased, the antimigration effects significantly increased in the 3DP and EPP. However, the direction of the flaps did not affect the MAMF. The position of the flaps attached to the surface of the stent affected the AMF.
Functional Evaluation of a “Lingual Ring” Oral Device Applied on Patients Affected by Temporo-Mandibular Disorders TMDs: A Comparative Clinical Trial
Authors: Currò Giuseppe , Ferlisi Mario , Rampello Alessandro , Rampello Alessio , Albergo Gianfranco
Published: May 2021

This study aims to evaluate the functional variations of the electromyographic response and clinical symptomatology in TMD patients. This study has been performed and compared before and after the application of a therapeutic protocol based on the use of an oral device working on the proper mandibular repositioning through the proprioceptive-based lingual re-education called “Lingual Ring”. Between January to December 2016, 32 TMD patients have been recruited out of a series of 321 individuals recruited at the Neurosensorial and Motorial Surgery Department, in Palermo (Italy). All the patients underwent the Surface Electromyography (sEMG) of Masseter and Temporal muscles, with different registrations at T0, T1, T2, and T3; to evaluate the variations of the Electromyographic values, it was assigned the application of the Lingual Ring as the only therapy. The study demonstrated a neat rebalancing of the EMG tracks and important improvements with the TMD related issues. The clinical responses, due to the treatment at the end of the therapeutic protocol, were: absence or reduction of muscular or articular pain; absence or reduction of articular noises; absence or reduction of the cephalalgia. Hence, significant results, both clinical and in terms of instrumental EMG, were assessed. We can affirm that the adopted methodology allowed the monitoring of the Electromyographic variation and clinical symptoms. Moreover, the usage of the “Lingual Ring device” allowed to carry out a simple and immediate therapeutic protocol that is minimally invasive, ensuring a clear rebalancing of the EMG tracks as well as the TMD related pain.
Reliability-Aware SPICE Compatible Compact Modeling of IGZO Inverters on a Flexible Substrate
Authors: Kim Je-Hyuk , Seo Youngjin , Jang Jun Tae , Park Shinyoung , Kang Dongyeon , Park Jaewon , Han Moonsup , Kim Changwook , Park Dong-Wook , Kim Dae Hwan
Published: May 2021

Accurate circuit simulation reflecting physical and electrical stress is of importance in indium gallium zinc oxide (IGZO)-based flexible electronics. In particular, appropriate modeling of threshold voltage (VT) changes in different bias and bending conditions is required for reliability-aware simulation in both device and circuit levels. Here, we present SPICE compatible compact modeling of IGZO transistors and inverters having an atomic layer deposition (ALD) Al2O3 gate insulator on a polyethylene terephthalate (PET) substrate. Specifically, the modeling was performed to predict the behavior of the circuit using stretched exponential function (SEF) in a bending radius of 10 mm and operating voltages ranging between 4 and 8 V. The simulation results of the IGZO circuits matched well with the measured values in various operating conditions. It is expected that the proposed method can be applied to process improvement or circuit design by predicting the direct current (DC) and alternating current (AC) responses of flexible IGZO circuits.
Near-Infrared Hyperspectral Imaging (NIR-HSI) for Nondestructive Prediction of Anthocyanins Content in Black Rice Seeds
Authors: Amanah Hanim Z. , Wakholi Collins , Perez Mukasa , Faqeerzada Mohammad Akbar , Tunny Salma Sultana , Masithoh Rudiati Evi , Choung Myoung-Gun , Kim Kyung-Hwan , Lee Wang-Hee , Cho Byoung-Kwan
Published: May 2021

Anthocyanins are an important micro-component that contributes to the quality factors and health benefits of black rice. Anthocyanins concentration and compositions differ among rice seeds depending on the varieties, growth conditions, and maturity level at harvesting. Chemical composition-based seeds inspection on a real-time, non-destructive, and accurate basis is essential to establish industries to optimize the cost and quality of the product. Therefore, this research aimed to evaluate the feasibility of near-infrared hyperspectral imaging (NIR-HSI) to predict the content of anthocyanins in black rice seeds, which will open up the possibility to develop a sorting machine based on rice micro-components. Images of thirty-two samples of black rice seeds, harvested in 2019 and 2020, were captured using the NIR-HSI system with a wavelength of 895–2504 nm. The spectral data extracted from the image were then synchronized with the rice anthocyanins reference value analyzed using high-performance liquid chromatography (HPLC). For comparison, the seed samples were ground into powder, which was also captured using the same NIR-HSI system to obtain the data and was then analyzed using the same method. The model performance of partial least square regression (PLSR) of the seed sample developed based on harvesting time, and mixed data revealed the model consistency with R2 over 0.85 for calibration datasets. The best prediction models for 2019, 2020, and mixed data were obtained by applying standard normal variate (SNV) pre-processing, indicated by the highest coefficient of determination (R2) of 0.85, 0.95, 0.90, and the lowest standard error of prediction (SEP) of 0.11, 0.17, and 0.16 mg/g, respectively. The obtained R2 and SEP values of the seed model were comparable to the result of powder of 0.92–0.95 and 0.09–0.15 mg/g, respectively. Additionally, the obtained beta coefficients from the developed model were used to generate seed chemical images for predicting anthocyanins in rice seed. The root mean square error (RMSE) value for seed prediction evaluation showed an acceptable result of 0.21 mg/g. This result exhibits the potential of NIR-HSI to be applied in a seed sorting machine based on the anthocyanins content.
An Experimental Study of a Nailed Soil Slope: Effects of Surcharge Loading and Nails Characteristics
Authors: Mohamed Mahmoud H. , Ahmed Mohd , Mallick Javed , Hoa Pham V.
Published: May 2021

The earth nailing system is a ground improvement technique used to stabilize earth slopes. The behavior of the earth nailing system is dependent on soil and nailing characteristics, such as the spacing between nails, the orientation, length, and method of installation of nails, soil properties, slope height and angle, and surcharge loading, among others. In the present study, a three-dimensional physical model was built to simulate a soil nailed slope with a model scale of 1:10 with various soil nail characteristics. The simulated models consist of Perspex strips as facing and steel bars as a reinforcing system to stabilize the soil slope. Sand beds in the model were formed, using a sand raining system. The performance of nailed soil slope models under three important nails characteristics, i.e., length, spacing and orientation, with varying surcharge loading were studied. It was observed that there is a reduction in the lateral movement of slope and footing settlements with an increase in length. It was found that the slope face horizontal pressure is non-linear with different nail characteristics. The increase in length and inclination of the soil nails decreased the vertical, horizontal stress and footing settlement, while the increase in spacing of the nails increased the vertical and horizontal stress behind the soil mass.
A GIS-Based Spatial Analysis Model Approach for Identification of Optimal Hydrotechnical Solutions for Gully Erosion Stabilization. Case Study
Authors: Bilașco Ștefan , Roșca Sanda , Vescan Iuliu , Fodorean Ioan , Dohotar Vasile , Sestras Paul
Published: May 2021

The accentuated degradation of agricultural lands as a result of deep erosion processes is the main problem identified in abandoned agricultural lands under the rainfall intensities, increasing number of hot days, indirectly under the impact processes derived from them (soil erosion, vegetation drying, etc.), as well as inadequate or poor management policies implemented by local authorities. The present study aims to develop and present a methodology based on GIS spatial analysis to choose the best hydro-amelioration solution for the arrangement of a complex ravine that negatively affects the entire agroecological area in its immediate vicinity. The proposed model is developed on spatial databases obtained based on UAV flights, the simulation of flow rate values and the establishment of three hydraulic analysis models through the HEC-RAS software with the main purpose of evaluating the results and databases, in order to identify the best implementing model for the stabilization and reduction in erosion within the analysed area. The comparative analysis of the three analysed scenarios highlighted the fact that a dam-type structure with overflow represents the best hydro-ameliorative solution to be implemented in the present study. The accuracy of the obtained results highlights the usefulness of developing GIS models of transdisciplinary spatial analysis to identify optimal solutions that can be implemented in territories with similar characteristics.