bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of environmental factors on accumulation of trace metal in a typical shale gas exploitation area: A comprehensive investigation by empirical and geodetector models
Authors: Bai Hongcheng , Li Yan , Lu Peili , Li Yutong , Zhang Lilan , Zhang Daijun , Wang Xing , Zhou Yuxiao
Published: August 2023

Whether a certain relationship is exist between shale gas exploitation and accumulation of trace metals in soil is a controversial issue in recent years. To date, few study clearly reveal the intrinsic correlations between environmental factors and accumulation of trace metals in soil of shale gas exploitation area. This study investigated the content and pollution level of trace metals in a typical shale gas exploitation area, and the relationships between environmental factors and spatial distribution of trace metals were evaluated. The results showed that there were 10.33%-25.87% proportion of soils were considered as pollution by trace metals, and the content of Ba contributed the most to the pollution index (Pn). Although SOM and CEC were considered as dominant factor influencing the content of trace metals, the results of MLR model based on natural factors strengthen the need for considering anthropogenic factors. Furthermore, the interaction between natural and ananthropogenic factors showed a siginifacnt nonlinear enhancement effect on accumulation of Cr, Ba and Sr, and the highest value of explanatory power (q) was 0.38 for SOM and distance. This study proved that the potential metal contamination was related to shale gas exploitation and provide reference for controlling soil pollution in shale gas exploitation area and making management strategy.
Poor Childhood Oral Health and Carotid Intima-Media Thickness: A Systematic Review
Authors: Alchourron Emilie , Asselin Marie-Ève , Fréchette Britta , Moretti Jean-Baptiste , El-Jalbout Ramy
Published: August 2023

Background: In adults, poor oral health, such as periodontal disease, is associated to increased circulating inflammatory markers and cardiovascular disease risk. To detect subclinical atherosclerosis, carotid intima-media thickness (IMT) is a radiological technique used in adults and children. A meta-analysis of 11 studies demonstrated the significant increase in IMT in adults in with periodontal disease.
Purpose: Examine the evidence concerning poor oral health and increased IMT in children.
Methods. Following the Cochrane Handbook of Systematic Reviews and PRISMA checklist, we conducted a systematic review. We searched on the Cochrane Database, MEDLINE, EMBASE, and CINHAL for publications in French and English, from inception until March 2023. Two researchers independently reviewed each article and applied inclusion and exclusion criteria to screen for eligibility. Risk of bias was also assessed using the GRADE approach.
Results. The literature search yielded 40 articles, and 2 were included. Mean age for both studies was 9.84 years (49.8% female), with an age range of 6-17 years old. Both studies concluded that an association exists between IMT and chronic buccodental status in children. Moreover, childhood oral infections were linked to subclinical atherosclerosis in adulthood.
Conclusion. This association has important clinical implications for treatment and preventive measures in evaluating the risk factors for early cardiovascular disease in children.
Retrospective data analysis of twenty work-related injured workers who received autologous tenocyte injection for chronic resistant lateral epicondylitis between 2011 to 2018. A clinical and economic perspective
Authors: Dey Sovan , Hughes Jeff , O’Beirne Alex , Wang Allan , Zheng MingHao , White Craig
Published: August 2023

Background: Lateral epicondylitis or tendinopathy is the most common cause of lateral elbow pain, and chronic resistant lateral epicondylitis (CRLE) is a problem for many workers. Among the non-surgical treatments, autologous tenocyte injection (ATI) is relatively new and may provide faster recovery.
Objectives- To assess WorkCover patients with CRLE who have undergone ATI rather than surgical intervention. To analyse their recovery period, pain score, functional capacity and changes in MRI findings. And to estimate any economic advantage of ATI over surgery.
Methodology: A retrospective data analysis was performed on 20 patients with work-related CRLE who received ATI. It evaluated their ability to return to work and their quality of life. Using Australian Medical Association and Medicare Benefits Schedule item numbers, an estimated cost comparison was made between non-operative ATI and the current surgical treatment.
Results: Of 20 workers (11 M/ 9 F) (mean age 47.1 years), at the time of ATI, 11 (55%) had limited work capacity, and 9 (45%) were absent from work. 80 % of workers with CRLE for more than 12 months returned to full duty (mean 6.6 months) following ATI. Estimated treatment cost and return to full duty duration were less with ATI than with surgery.
Conclusion: Overall treatment success with ATI is promising, with earlier return to work, improved functionality and cost-effectiveness compared to surgery.
TLR4-mediated macropinocytosis promotes smooth muscle cell- derived foam cell formation
Authors: Chen Xue , Kang Yulai , Li Jingcheng , Tang Chunhua , Guo Lu , Zhang Lili
Published: August 2023

Objective: To investigate whether vascular smooth muscle cells (VSMCs) take up lipids via the Toll-like receptor 4/spleen tyrosine kinase (TLR4/Syk) mediated macropinocytosis pathway and promote the formation of lipid droplets in cells. Methods: Primary wild-type (WT) and TLR4 gene-knockout (TLR4−/−) VSMCs were isolated by an adherent tissue culture method. Changes in VSMCs uptake of DiI-labelled native low-density lipoprotein (DiI-nLDL) and Lucifer yellow (LY) were detected by flow cytometry. The colocalization of boron-dipyrromethene (BODIPY)-labelled Neutral lipid and LY-labelled macropinosomes in VSMCs was observed by confocal microscopy.
Results: Lipopolysaccharide (LPS)induced nLDL uptake by WT VSMCs, resulting in the accumulation of lipid droplets and the formation of WT VSMC-derived foam cells. Stimulation of WT VSMCs with 200 ng/ml LPS resulted in increase in LY and DiI-nLDL uptake, lipid droplets and macropinocytosis were colocalized in WT VSMCs, but this above effect was significantly reduced in WT VSMCs after treatment with macropinocytosis inhibitors, and was not observed in TLR4−/− VSMCs. WT VSMCs showed a decrease in LY and DiI-nLDL uptake after treatment with 2 µmol/L R788.
Conclusion: LPS induces nLDL uptake by VSMCs via the macropinocytosis pathway to promote lipid droplet aggregation and the formation of VSMC-derived foam cells. TLR4/Syk is an important molecule associated with VSMC uptake of nLDL through the macropinocytosis pathway.
Forest Types for Afforestation: Benefits for Carbon Sequestration and Food Systems under Stringent Climate Mitigation
Authors: Hasegawa Tomoko , Fujimori Shinichiro , Ito Akihiko , Takahashi Kiyoshi
Published: August 2023

Afforestation is considered as a major carbon dioxide removal (CDR) technology, but if implemented inappropriately in large scale, can negatively affect food and land systems. Here we quantitatively showed how a forest-type selection in afforestation would enlarge the global carbon sequestration and affect global food and land systems and sustainability. We found that i) afforestation, if its forest type is carefully selected, would increase the carbon sequestration by 25% at maximum compared to the indigenous type of forest while reducing food insecurity. At the same time, ii) compared to bioenergy with carbon capture and storage, afforestation would affect similarly or even more negatively the economy, energy, food, and land systems due to less land efficiency of carbon removal, leading higher price of energy and food, more land expansion for carbon removal and higher food insecurity. This suggests a necessity of a careful selection of forest types in afforestation and at the same time an importance of determining a best mix of land-based CDR technologies to achieve the long-term stringent climate goal without compromising the sustainability.
Effect of radiation therapy on lymph node fluorescence in head and neck squamous cell carcinoma after intravenous injection of indocyanine green: A prospective evaluation
Authors: digonnet antoine , vankerkhove Sophie , Moreau Michel , Dekeyser Cécile , Quiriny Marie , Willemse Esther , aubain Nicolas de saint , cappello Matteo , Donckier vincent , bourgeois pierre
Published: August 2023

Background:
Indocyanine green (ICG)-guided surgery has proven effective in the identification of neoplastic tissues. The effect of radiation therapy (RT) on lymph node fluorescence after intravenous injection of ICG has not been addressed yet. The objective of this study was to evaluate the influence of RT on node fluorescence during neck dissection in head and neck squamous cell carcinoma (HNSCC).
Results:
Twenty-four patients with planned neck dissection for HNSCC were prospectively enrolled. Eleven were included without previous radiation therapy and 13 after RT. ICG was intravenously administered in the operating room. The resected specimen was analyzed by the pathology department to determine the status of each resected lymph node (invaded or not). The fluorescence of each resected node was measured in arbitrary units (AU) on paraffin blocs. The surface area (mm2) of all metastatic nodes and of the invaded component were measured. The values of these surface areas were correlated to fluorescence values.
A total of 707 nodes were harvested, the mean fluorescence of irradiated nodes (n=253) was 9.2 AU and of non-irradiated nodes (n=454) was 9.6 AU (p=0.63). Fifty nodes were invaded, with a mean fluorescence of 22 AU. The mean fluorescence values in the invaded irradiated nodes (n=20) and the invaded non-irradiated nodes (n=30) were 19 AU and 28 AU (p=0.23), respectively. The surface area of metastatic nodes and of the invaded component were correlated to fluorescence values even after previous RT (p=0.02).
Conclusion:
No differences were observed between the fluorescence of irradiated and non-irradiated lymph nodes, including invaded nodes. ICG-guided surgery can be performed after failed RT.
Enhanced 3D visualization based on the inverse modeling of non-parallel 2D ERT profiles: An approach for assessing anomalous zone
Authors: Mishra Utsav , Bakshi Ansuman , Mandal Animesh
Published: August 2023

Three-dimensional resistivity models based on observed data in a grid pattern aids in understanding the volume and positional relations of subsurface regions having resistivity contrast with respect to the surrounding medium. However, acquiring ERT profiles in a regular 2D grid is labor intensive, time-consuming, and costly process. To overcome these constraints, pseudo 3D visualizations generated from multiple parallel 2D resistivity profiles are often used. Moreover, acquiring parallel 2D resistivity profiles in a grid is also often constraints by logistical challenges such as presence of utilities, waste mounds waterlogged patches etc. Therefore, in this work, a methodology is devised for creating a 3D database from inverse modeling results of multiple 2D resistivity profiles oriented in random direction in a region. In this approach, the azimuths of randomly oriented profiles are measured and utilized for introducing the y-coordinate. The proposed methodology is tested on 2D ERT datasets acquired at a chromite ore processing residue (COPR) waste dumping site.3D models generated through this approach has provided an enhanced visualization of conductive anomalous zones (i.e., < 15 Ohm.m) originating from the waste dump site. Further, the results obtained from the proposed methodology is compared with the quasi-3D volume generated by applying the 3D inversion algorithm on the compiled 2D ERT profiles datasets. The 3D visualization based on the proposed approach shows better correlation with anomalous zones present in the 2D inversion results of individual profiles. Thus, the methodology provides an efficient tool for creating 3D visualization models, and can be generalized for existing approaches...
Examining sustainable development goals: Are developing countries advancing in sustainable energy and environmental sustainability?
Authors: Hossin Md Altab , Abudu Hermas , Sai Rockson , Agyeman Stephen Duah , Wesseh Presley K.
Published: August 2023

Research findings suggest that understanding the complex interplay between sustainable indicators requires comprehensive intelligence and modeling. However, the availability of unified database for these purposes remains a challenge. This study contributes new insights to the existing literature utilizing 32 indicators of sustainable development goals (SDGs) to compute six composite indicators. The study applies dynamic panel analysis (generalized method of moments) and the Probit model to investigate the study in ten selected developing countries, using data from 2012 to 2020. The results indicate that since the adoption of SDGs in 2015, low and middle-income countries have made progress in improving water and sanitation sustainability, leading to enhanced environmental quality. Additionally, middle-income countries have shown greater advancements in sustainable energy compared to low-income countries, contributing to overall environmental sustainability. However, middle-income countries lag behind in sustainable agriculture when compared to their low-income counterparts. These findings highlight the potential adverse effects of SDG policy strategies, particularly in middle-income countries, where agriculture-based energy sources such as biomass and biofuels negatively impact sustainable agriculture outcomes. Furthermore, the study reveals a negative impact of carbon footprint on sustainable agriculture in developing countries since the adoption of the SDGs. Based on these findings, the study puts forward policy recommendations, including the development of unified database to support research on SDGs.
A new modified laparoscopic lateral suspension with mesh for apical and anterior pelvic organ prolapse: clinical analysis of 51 cases
Authors: Xu Sheng , Nie Jingya , Zeng Wenjie , Chen Xiaoyan , Bai Xuechai , Yu Yan
Published: August 2023

Background: To evaluate the efficacy of a modified laparoscopic lateral suspension with mesh (mLLSM) in patients with apical and anterior pelvic organ prolapse.
Methods: All patients diagnosed with apical and anterior pelvic organ prolapse underwent a modified laparoscopic lateral suspension with mesh (mLLSM). Perioperative parameters including surgical time, blood loss and complications were recorded. At the minimal 12-month follow-up, primary outcome measures included both anatomical and functional points. The anatomical cure rate was evaluated using the Pelvic Organ Prolapse Questionnaire (POP-Q) assessment. Patient satisfaction was evaluated using Questionnaires.
Results: Mean surgical time was 91.56 ± 15.33 min; mean estimated blood loss was 55.42 ± 36.73 ml; No intraoperative complications were noted in the perioperative period. After a minimal 12-month follow-up period, rates of anatomical success and subjective satisfaction were 96.08% and 94.12%, respectively. Symptom severity and quality of life also improved significantly.
Conclusion: We found mLLSM be a safe and effective treatment for patients suffering apical and anterior pelvic organ prolapse. We found mLLSM to result in excellent outcomes and fewer mesh complications, underscoring its potential as an alternative treatment option for the management of apical and anterior pelvic organ prolapse.
The predictive effect of red cell distribution width on hypertensive disorders of pregnancy: A propensity score-matching study
Authors: Lv Bin , Han Lin , Chen Yali , Liu XH , Chen Hengxi
Published: August 2023

Background
Red cell distribution width (RDW) is found to be related to inflammation and oxidative stress, and gradually used as a predictor of in cardiovascular diseases. This study aimed at investigated the predicting effects of RDW on hypertensive disorders of pregnancy (HDP).
Methods
This is a retrospective, propensity score-matching (PSM) cohort study. Cohort 1 studied patients with gestational hypertension/chronic hypertension (named the simple hypertension group), while cohort 2 studied patients with preeclampsia (including preeclampsia, severe preeclampsia, and chronic hypertension patients with preeclampsia). Univariate logistic regression analysis was conducted on variables that were not corrected after PSM. Receiver operating characteristic (ROC) curves were used to explore the predictive ability of RDW for HDP.
Results
After 1:1 PSM, 1546 women admitted to West China Second University Hospital between January 2021 and January 2022 were enrolled in the analysis, including 420 in the simple hypertension group, 353 in the preeclampsia group, and the remaining 773 in the control group. Univariate logistic regression analysis showed that although there was no significant correlation between RDW and simple hypertension (gestational hypertension and chronic hypertension), there was a significant correlation between RDW and preeclampsia. The ROC curve analysis showed a diagnostic accuracy of RDW coefficient variation (RDWCV) for severe preeclampsia at 72%, with a cutoff value of ≥ 14.65% and AUC = 0.696, and a diagnostic accuracy of RDW standard deviation (RDWSD) for severe preeclampsia at 76%, with a cutoff value of ≥ 51.85fl and AUC = 0.661.
Conclusion
RDW is significantly correlated with preeclampsia and had a good predictive effect on severe preeclampsia (AUC = 0.7). RDW has the advantages of being fast, low-cost, and easily available, so it can be routinely used for predicting preeclampsia in clinical practice.
Plasma Inflammation and Plasticity Biomarkers Associated with Future Suicidal Ideation and Depression Severity in Mood Disorders: A Six-Month Prospective Study
Authors: Lengvenyte Aiste , COGNASSE Fabrice , Hamzeh-Cognasse Hind , Sénèque Maude , Strumila Robertas , Olié Emilie , Courtet Philippe
Published: August 2023

Discerning potential associations between plasma biomarkers and the evolution of suicidal ideation (SI) in mood disorders could advance our understanding of disease progression. We evaluated a cohort of 149 mood disorder patients, assessing 32 plasma soluble proteins implicated in neuroplasticity and inflammation at baseline and six-month follow-up. The primary outcome was the occurrence of suicidal ideation during the six-month follow-up. Secondary outcomes included the presence of suicidal ideation and depression severity at six-month follow-up. We employed Principal Component Analysis and Elastic Net regression for feature extraction and selection. Selected markers were then examined in covariate-adjusted regression models. Our results showed that high baseline levels of interferon-γ and a pro-inflammatory principal component score were linked to the occurrence of SI during follow-up. At the six-month point, SI presence was associated with elevated interferon-γ, interleukin-1β, and diminished serotonin levels, with the latter connection dissipating post-adjustment. High interferon-γ, and low orexin-A at baseline were associated with increased depression severity at follow-up, with specific associations between interferon-γ and anxious symptoms, and orexin-A and atypical depressive symptoms. These findings identify elevated interferon-γ, interleukin-1β, and reduced orexin-A as potential biomarkers associated with the occurrence of SI and severity of depression in mood disorders over six months. With further validation in larger cohorts, these insights could enable more personalized risk assessment and intervention strategies, representing a step forward in improving therapeutic outcomes.
Post-treatment peripheral blood markers as predictive indicators for immune related thyroid dysfunction and outcome in non-small cell lung cancer patients receiving PD-1 inhibitor
Authors: Liu Jinyu , Qin Boyu , Zhang Xiaoxuan , Cai Le , Bai Nan , Tang Zhihui , Chen Mengli , Wang Tianlin
Published: August 2023

Methods
A retrospective study of 133 non-small cell lung cancer (NSCLC) patients treated with PD-1 inhibitors was performed. Medical records and haematological data were collected. The trends of NLR and PLR during treatment were analysed by the Mann-Whitney U test. Logistic regression was used to investigate the association of irTD, all immune-related adverse events (irAEs) with pre- and post-treatment NLR and PLR. The relationship of these predictive factors with tumour response and survival was estimated using the Chi-squared test or Kaplan‒Meier analysis.
Results
30 patients (22.6%) developed irTDs. The NLR showed a significant decreasing trend during treatment. Patients with previous thyroid diseases and lower NLR3w (NLR < 2.3) were significantly associated with irTDs. Pretreatment lower NLR and lower NLR3w were associated with irAEs. There was no difference in tumor response between patients with and without irTDs. PFS was prolonged in patients with irTDs and with lower PLR7w.
Conclusions
IrTD onset is common in patients receiving PD-1 inhibitors and is associated with prolonged PFS. The NLR3w after receiving PD-1 inhibitors appears to be a factor for identifying NSCLC patients at high-risk of occurring irTD. Post-treatment lower PLR is associated with better PFS.
Enhancing ReaxFF for Molecular Dynamics Simulations of Lithium-Ion Batteries: An interactive reparameterization protocol
Authors: Chiavazzo Eliodoro , De Angelis Paolo , Cappabianca Roberta , Fasano Matteo , Asinari Pietro
Published: August 2023

Lithium-ion batteries (LIBs) have become an essential technology for the green economy transition, as they are widely used in portable electronics, electric vehicles, and renewable energy systems. The solid-electrolyte interphase (SEI) is a key component for the correct operation, performance, and safety of LIBs.
The SEI arises from the initial thermal metastability of the anode-electrolyte interface, and the resulting electrolyte reduction products stabilize the interface by forming an electrochemical buffer window.
This article aims to make a first -- but important -- step towards enhancing the parametrization of a widely-used reactive force field (ReaxFF) for accurate molecular dynamics (MD) simulations of SEI components in LIBs.
To this end, we focus on Lithium Fluoride (LiF), an inorganic salt of great interest due to its beneficial properties in the passivation layer.
The protocol relies heavily on various Python libraries designed to work with atomistic simulations allowing robust automation of all the reparameterization steps.
The proposed set of configurations, and the resulting dataset, allow the new ReaxFF to recover the solid nature of the inorganic salt and improve the mass transport properties prediction from MD simulation.
The optimized ReaxFF surpasses the previously available force field by accurately adjusting the diffusivity of lithium in the solid lattice, resulting in a two-order-of-magnitude improvement in its prediction at room temperature.
However, our comprehensive investigation of the simulation shows the strong sensitivity of the ReaxFF to the training set, making its ability to interpolate the potential energy surface challenging.
Consequently, the current formulation of ReaxFF can be effectively employed to model specific and well-defined phenomena by utilizing the proposed interactive reparameterization protocol to construct the dataset.
Overall, this work represents a significant initial step towards refining ReaxFF for precise reactive MD simulations, shedding light on the challenges and limitations of ReaxFF force field parametrization.
The demonstrated limitations emphasize the potential for developing more versatile and advanced force fields to upscale ab initio simulation through our interactive reparameterization protocol, enabling more accurate and comprehensive MD simulations in the future.
A study on garbage code redistribution methods for heart failure at city level by two approaches
Authors: Liu Liqun , Cai Zemin , Wang Xuewei , Wang Chunping , Ma Xiangyun , Meng Xianfeng , Ning Bofu , Li Ning , Wan Xia
Published: August 2023

Background
Garbage code (GC) in death surveillance data may affect the statistics on causes of death. The Global Burden of Disease (GBD) studies have proposed several methods to redistribute GCs to the plausible correct underlying cause of death (UCOD). Take heart failure as an example, this study aimed to explore suitable GC redistribution methods at the city level.
Methods
The cause of death surveillance data was from Weifang city, from 2010 to 2017, and Xuanwei county, from 2010 to 2016, China. Firstly, the death records of heart failure were corrected to UCOD based on the World Health Organization (WHO) guidelines. Secondly, the records with UCOD remaining to be heart failure were proceeded by coarsened exact matching (CEM) and linear regression (LR), respectively. Finally, the change of cause-specific mortalities before and after redistributed by two methods was calculated, respectively.
Results
The UCOD stated as heart failure was 1556 (0.33%) in Weifang and 226 (0.41%) in Xuanwei, respectively. After redistribution based on the WHO guideline, around 75% of the UCOD records in both cities remained the same. In Weifang, by CEM, heart failure was mainly redistributed to ischemic heart disease (IHD, 45.31%) and hypertensive heart disease (HHD, 21.56%). By LR, 91.20% of heart failure was redistributed to IHD. In Xuanwei, by CEM, heart failure was mainly redistributed to IHD (24.70%), diabetes mellitus and chronic kidney disease (DMCKD 23.25%). By LR, 94.83% of heart failure was redistributed to chronic obstructive pulmonary disease (COPD).
Conclusions
During conducting GC redistribution, careful consideration of which method to choose is necessary, especially for the city-level data. In this study, the CEM approach might probably be more suitable for the city level, compared to LR.
Modeling of Effects of Vibration Parameters on Location-Dependent Initial Relative Density in Powder Metallurgy Capsule Filling
Authors: Sobhani Samaneh , Gandy David , Albert Marc , Tabei Ali , Fan Zhaoyan
Published: August 2023

Power Metallurgy Hot Isostatic Pressing (PM-HIP), as a versatile manufacturing process, has the ability to produce net-shape or near-net-shape components with complicated geometries from materials that are not easily cast, deformed, or welded. In PM-HIP, capsule filling is a critical step to get dimensionally and microstructurally sound outputs. Particularly, capsule filling controls the initial relative density (homogeneity) of the PM-HIP compact. In this study, the pre-consolidation capsule filling process is simulated by the Discrete Element Method (DEM); to capture the impact of vibration parameters, including frequency, amplitude, and vibration direction, on the initial Relative Density (RD). The output of the DEM model was imported into a user subroutine-based finite element of PM-HIP containing a combined constitutive model of compressive and consolidative mechanical behavior of powder. The simulation model was used to quantitatively study the relationships between the vibration parameters and the initial RD of the product. The ultimate results of this work show that the optimal initial RD is obtained at the frequency of 60 Hz. The FEA results illustrate that the compact densifies accordingly with respect to the RD and the uniformity of its distribution.
Distribution, local knowledge and uses of stingless bees (Apidae: Meliponini) assessment and its honey physico-chemical property in selected Woredas of South Omo Zone.
Authors: Ayele Bizuayehu Abebe
Published: August 2023

The study was conducted in Southern Region South Omo Zone Southern Ethiopia to understand agro-ecological distribution of stingless bee and their management practices, socio-economic importance of stingless bees and characterize the physico-chemical properties of honey produced from stingless bees (Meliponula beccarii). Purposive sampling techniques were used to select participants in the FDG. Three FGD per district comprised with 10–12 stingless bee keepers were undertaken for understanding of agro ecological distribution, local knowledge, management practices and socio-economic importance of stingless bees. Honey samples were collected from underground soils through an excavation of natural nests from three representative districts namely Debub Ari, Malee and Bena-Tsemay districts and about 15 honey samples 0.5 liter from each nest was collected for physico-chemical analysis. The two recorded stingless bee species in the study area were Meliponula beccarii and Hypotrigona gribodoi. The two species of stingless bees are varied within their body size, nesting habitats, nest structure and nest site. The productivity of stingless bees varies depending on species, availability of bee forage and the size of the stingless bee colony population. The laboratory result of locally produced stingless bee honey had moisture content, pH, acidity, ash content, Hydroxy Methyl Furfural (HMF), Fructosr, Glucose and Sucrose average contents were 32.59 percent, 3.42 per cent, 113.57 meq/kg, 0.27 g/100 g, 3.51 mq/kg, 29.11%, 29.29% and 2.78%, respectively. Stingless bee keeping practicing in the backyard through trained the beekeepers and using of naturally designed model stingless bee hives.
More may not be better: Enhanced spacecraft shielding may exacerbate cognitive decrements by increasing pion exposures during deep space exploration
Authors: Limoli Charles , VOZENIN MARIE-CATHERINE , Alaghband Yasaman , Drayson Olivia , Piaget Filippo , Leavitt Ron , Allen Barrett , Doan Ngoc-Lien , Rostomyan Tigran , Stabilini Alberto , Reggiani Davide , Hajdas Wojciech , Yukihara Eduardo , Norbury John , Bailat Claude , Desorgher Laurent , Baulch Janet
Published: August 2023

The pervasiveness of deep space radiation remains a confounding factor for the transit of humans through our solar system. Spacecraft shielding both protects astronauts but also contributes to absorbed dose through galactic cosmic ray interactions that produce secondary particles. The resultant biological effects drop to a minimum for aluminum shielding around 20 g/cm2but increase with additional shielding. The present work evaluates for the first time, the impact of secondary pions on central nervous system functionality. The fractional pion dose emanating from thicker shielded spacecraft regions could contribute up to 10% of the total absorbed radiation dose. New results from the Paul Scherrer Institute have revealed that low dose exposures to 150 MeV positive and negative pions, akin to a Mars mission, result in significant, long-lasting cognitive impairments. These surprising findings emphasize the need to carefully evaluate shielding configurations to optimize safe exposure limits for astronauts during deep space travel.
Diagnostic Value of LGE and T1 mapping in Multiple Myeloma patients’heart
Authors: Cui Qian , Yu Jing , Ge Xihong , Gao Guangfeng , Liu Yang , He Qiang , Shen Wen
Published: August 2023

Background
Unidentified heart failure occurs in patients with multiple myeloma when their heart was involved. CMR with late gadolinium enhancement (LGE) and T1 mapping can identify myocardial amyloid infiltrations.
Purpose
To explore the role of CMR with late gadolinium enhancement (LGE) and T1 mapping for detection of multiple myeloma patients’heart.
Material and Methods
A total of 16 MM patients with above underwent CMR (3.0-T) with T1 mapping (pre-contrast and post-contrast) and LGE imaging. In addition, 26 patients with non-obstructive hypertrophic cardiomyopathy and 26 healthy volunteers were compared to age- and sex-matched healthy controls without a history of cardiac disease, diabetes mellitus, or normal in CMR. All statistical analyses were performed using the statistical software GraphPad Prism. The measurement data were represented by median (X) and single sample T test was adopted. Enumeration data were represented by examples and tested was adopted. All tests were two-sided, and P values < 0.05 were considered statistically significant.
Results
In MM group, LVEF was lower than healthy controls and higher than that of non-obstructive hypertrophic cardiomyopathy group, but without statistically significant difference ((%: 49.1 ± 17.5 vs. 55.6 ± 10.3、40.4 ± 15.6, all P > 0.05). Native T1 values of MM group were obviously higher than those of healthy controls and non-obstructive hypertrophic cardiomyopathy group (ms:1462.0 ± 71.3vs. 1269.3 ± 42.3、1324.0 ± 45.1, all P < 0.05). 16 cases (100%) in MM group all had LGE.
Conclusion
LGE joint T1 mapping examination can improve diagnostic accuracy and is extremely meaningful for patient diagnosis and follow-up.
Aberrant Overexpression of Myosin 1b in Glioblastoma Promotes angiogenesis via VEGF-myc-myosin 1b- Piezo1 Axis
Authors: Lv Autor：Weifeng , Yang Fan , Ge Zhengmao , Xin Lele , Zhang Lingxue , Guo Qingdong , Mao Xinggang , Luo Peng , Jiang Xiaofan , Zhang Yanyu
Published: August 2023

Glioblastoma (GBM) is the most aggressive intracranial malignance with poor prognosis, which is attributed to the extreme invasiveness of the tumor. Enhanced angiogenesis is one of the essential hallmarks of GBM, which demonstrates extensive microvascular proliferation and abnormal vasculature. Here, we uncovered the key role of myosin 1b in angiogenesis and vascular abnormality in GBM. Myosin 1b was upregulated in GBM endothelial cells (ECs) compared to their paired non-malignant brain tissue. Knocking down myosin 1b in human/mouse brain endothelial cells inhibited EC migration, proliferation and tube formation. Myosin 1b in ECs are affected by vascular endothelial growth factor (VEGF) signaling through myc. Moreover, myosin 1b promotes angiogenesis via Piezo1 by enhancing Ca2+ influx, in which process VEGF can be the trigger. Our results identified myosin 1b as a key mediator in promoting angiogenesis via Piezol1; suggested that VEGF/myc signaling pathway may be responsible for driving the changes of myosin 1b overexpression in GBM ECs.
Internal fixation of Vancouver B2 periprosthetic femoral fractures: Can it be an alternative to the revision arthroplasty?
Authors: Choi Jung Hwan , Hwang Kyu Tae , Sohn Oog-Jin , Kook Incheol , Park Ki-Chul
Published: August 2023

This study evaluated outcomes of open reduction and internal fixation (ORIF) for the treatment of Vancouver B fracture and analyzed factors affecting prognosis of internal fixation in Vancouver B2 fractures. From January 2009 to August 2021, 14 Vancouver B1 and 22 Vancouver B2 fractures that underwent ORIF were investigated. Clinical outcomes of adequacy of fracture reduction, duration of bone union, femoral stem subsidence were evaluated as well as revision rates and postoperative complications. The average union time for B1 was 14.5 weeks (7–25), the average union time for B2 was 21.0 weeks (8–36). (P = 0.053) Fracture reduction was poor in 1 case in B1 and 10 cases in B2. (p = 0.025) Stem subsidence occurred in 6 cases and revision surgery in 3 cases, all of which occurred in the poor reduction group. Of the 6 cases of stem subsidence, 3 cases were cemented stem and all cements were broken. (p = 0.046). ORIF on Vancouver B2 fracture showed relatively satisfactory results, so it is expected to be an alternative to revision arthroplasty in selected Vancouver B2 fracture. Accurate anatomical reduction during surgery is important to avoid reoperation and femoral stem subsidence. Revision would be better than internal fixation in mantle broken cemented stem Vancouver B2 fracture.
Impact of Negative Emotions on Upper Gastrointestinal Diseases: A Mendel Randomization Study
Authors: Gao Nan , Yu Zhun , Fan Yu , Jiang Xue , Ting Hu
Published: August 2023

Previous observational studies have reported a relationship between negative emotions and upper gastrointestinal disease. However, whether this relationship is causal remains unclear. We aimed to evaluate the causal relationship between negative emotions and upper gastrointestinal diseases using two-sample Mendelian randomization (MR). Three sets of genetic instruments from the database were obtained for analysis, including 12 anxiety-related single nucleotide polymorphisms (SNPs), 46 depression-related SNPs, and 58 nervous-related SNPs. SNPs were filtered using the Phenoscanner website, and the inverse variance weighted method, weighted median method, MR-Egger regression, MR pleiotropy residual sum, and outlier test were used for analysis. In inverse variance weighted analysis, anxiety and depression had an effect on gastroduodenal ulcer (p = 2.849 × 10-3, β = 4.908, 95% CI = 1.684–8.132; and p = 6.457 × 10-4, β = 1.767, 95% CI = 0.752–2.782, respectively). Additionally, depression had an effect on diseases of the esophagus, stomach, and duodenum (p = 3.498 × 10-5，β = 0.926，95% CI = 0.487–1.364). Cochran’s Q-derived p-values were 0.457, 0.603, and 0.643, and MR-Egger intercept-derived p-values were 0.697, 0.294, and 0.362, respectively. Here, we show that anxiety and depression have a causal relationship with gastroduodenal ulcers, and depression has a causal relationship with diseases of the esophagus, stomach, and duodenum.
Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders
Authors: gao Shan , Zhao Xiaoshuai , Leng Yan , Xia Zhongyuan
Published: August 2023

Inulin, as a prebiotic, could modulate the gut microbiota. Burn injury leads to gut microbiota disorders and skeletal muscle catabolism. Therefore, whether inulin can improve burn-induced muscle atrophy by regulating microbiota disorders remains unknown. This study aimed to clarify that inulin intake alleviates gut microbiota disorders and skeletal muscle atrophy in burned rats. Rats were divided into the sham group, burn group, prebiotic inulin intervention group, and pseudo-aseptic validation group. A 30% total body surface area (TBSA) third-degree burn wound on dorsal skin was evaluated in all groups except the sham group. Animals in the intervention group received 7 g/L inulin. Animals in the validation group received antibiotic cocktail and inulin treatment. In our study inulin intervention could significantly alleviate the burn-induced skeletal muscle mass decrease and skeletal myoblast cell apoptosis. Inulin intake increased the abundances of Firmicutes and Actinobacteria but decreased the abundance of Proteobacteria. The biosynthesis of amino acids was the most meaningful metabolic pathway distinguishing the inulin intervention group from the burn group, and further mechanistic studies have shown that inulin can promote the phosphorylation of the myogenesis-related proteins PI3K, AKT and P70S6K and activate PI3K/AKT signaling for protein synthesis. In conclusion, inulin alleviated burn induced muscle atrophy through PI3K/AKT signaling and regulated gut microbiota dysbiosis.
Sex differences in the association between epicardial adipose tissue volume and left atrial volume index
Authors: Yamaguchi Satoshi , Maeda Minetaka , Oba Kageyuki , Maimaituxun Gulinu , Arasaki Osamu , Yagi Shusuke , Kusunose Kenya , Soeki Takeshi , Yamada Hirotsugu , Fukuda Daiju , Masuzaki Hiroaki , Sata Masataka , Shimabukuro Michio
Published: August 2023

Background: Sex disparities in the association between epicardial adipose tissue volume (EATV) and cardiovascular disease have been reported. The sex-dependent effects of EATV on left atrial (LA) function have not been elucidated.
Methods: Consecutive 247 subjects (median 65 [interquartile range 57, 75] years; 67% of men) who underwent multi-detector computed tomography without significant coronary artery disease or moderate to severe valvular disease were divided into two groups: patients with sinus rhythm (SR) or atrial fibrillation (AF). Sex differences in the association between the EATV index (EATVI) (mL/m2) and LA volume index (LAVI) in 63 SR (28 men and 35 women) and 184 AF (137 men and 47 women) patients were evaluated using univariate and multivariate regression analyses.
Results: In overall that includes both men and women, the relationship between EATVI and LAVI was not significantly correlated for patients with SR and AF. The relationship between EATVI and LAVI differed between men and women in both SR and AF groups. In SR patients, there was a positive relationship between EATVI and LAVI in men, but not in women. In contrast, in patients with AF, a negative relationship was found between EATVI and LAVI in women, whereas no association was found in men.
Conclusions: We evaluated sex differences in the association between EATVI and LAVI in patients with either SR or AF, and found a positive relationship in men with SR and a negative relationship in women with AF. This is the first report to evaluate sex differences in the relationship between EATVI and LAVI, suggesting that EAT may play a role, at least in part, in sex differences in the etiology of AF.
Sex Differences in the Association of the Severity of Childhood Sexual Abuse with Premarital Sex among Chinese College Students in Luzhou, China
Authors: Zhang Rong , He Jing , Yang Lin , Cao Rongzhe , Liao Maoxu , Lin Xin , Zhou Ping
Published: August 2023

Purpose:The adverse health consequences of premarital sex and childhood sexual abuse (CSA) are both global public health problems. Based on random samples of college students from a Chinese city, this study investigated the relationship between CSA severity and premarital sex among students, with a focus on sex differences.
Methods: A total of 2722 college students from 4 schools in Luzhou were recruited by multistage random sampling. Self-administered questionnaires were used to measure CSA experiences and premaritalsex. Binary logistic regression analyses were conducted to analyze the relationship between CSA and premaritalsex.
Results: The prevalence of CSA was 9.39%, and that of mild, moderate and severe CSA was 4.04%, 2.90% and 2.46% respectively. The premarital sex reporting rate was 22.42%. After adjusting for confounding variables, CSA was positively associated withpremarital sex. Notably, a cumulative effect of CSA on premarital sex was observed among students. Further stratification analyses showed that males who experienced CSA had a higher premarital sex rate than females, and this sex difference was also observed among students with different CSA severity.
Conclusion: CSA and its severity were associated with premarital sex among college students. Furthermore, this association was stronger for males than females. Therefore, it is important to emphasize CSA prevention, especiallyfor boys. These findings can promote understanding of the effects of CSA on premarital sex, and prevention and intervention strategies for CSA should consider CSAseverity and sexdifferences.
Iatrogenic Female Genitourinary Fistula in Uganda: Etiology, Twelve-year trends, and Risk factors among women delivered by cesarean section
Authors: Byamukama Onesmus , Tarnay Christopher , Ainomugisha Brenda , Tibaijuka Leevan , Kajabwangu Rogers , Kalyebara Paul Kato , Lugobe Henry Mark , Geissbühler Verena , Kayondo Musa
Published: August 2023

Background: More than 95% of genito-urinary fistulae in low-income countries are due to prolonged neglected obstructed labour. However, with improving obstetric care and variations in medical training, there seems to be an increase in fistulae resulting from surgical errors.
Objective: The aim of this study was to describe the magnitude and trend of iatrogenic genitourinary fistula at four fistula surgery sites in Uganda from 2010 to 2021 and also determining the risk factors of iatrogenic genitourinary fistula following Cesarean Section (CS).
Methods: A retrospective review of charts for all women who underwent surgery for genitourinary fistula at four regional fistula repair sites in Uganda between 2010 and 2021 was conducted. A fistula was classified as iatrogenic if a participant had any of; ureteric, vesico-cervical, vesico-uterine, or vaginal vault fistula that followed an obstetric or gynecological surgery. Vesico-vaginal and urethro-vaginal fistulae were classified as ischemic irrespective of the mode of delivery. The annual proportions for the obstetric and iatrogenic fistula over the 12-year period were compared. Descriptive analyses and multivariable log binomial regression were performed to determine the independent risk factors.
Results: Over the study period, 521 women had surgery for genito-urinary fistula of which, 169 (32.4%) were iatrogenic. Most of the iatrogenic fistulae followed CS (71%). There was a gradual increase in the proportions of iatrogenic fistulae from 8/52 (9.6%) in 2010 to 38/88 (43.2%) in 2020. The risk factors for iatrogenic fistula following CS were; Grand-multiparity (RR = 5.79; 95% CI: 2.18-15.4; P = 0.001), repeat CS (RR = 4.06; 95% CI: 1.77-9.3; P = 0.001), CS performed by an intern doctor (RR = 4.85; 95% CI: 1.52-15.45; P = 0.008) and CS done at a Health Centre IV (RR = 4.55; 95% CI: 1.2-16.67; P= 0.022).
Conclusion: The magnitude of iatrogenic genitourinary fistula in Uganda is high and most follow CS. There is an observed rising trend in iatrogenic fistula over time. The risk factors for iatrogenic fistula following CS are grandmultiparity, repeat CS, CS performed by intern doctors and CS performed at lower health facilities. There is need for routine in service surgical skills (especially cesarean sections) training for obstetric and gynecological surgery providers.
Rehabilitating instead of rebuilding aged or damaged pre-fabricated concrete buildings in Ukraine: a case for reducing CO2 emissions
Authors: Troian Viacheslav , Volodymyr Gots , Flatt Robert J. , Angst Ueli
Published: August 2023

Recycling the concrete of old and destroyed buildings and using it to build new buildings is a promising technology for post-war Ukraine, but in many cases, it may be economically and environmentally more justified to rehabilitate such buildings.
This article provides an analysis of the state of the housing stock of Ukraine from prefabricated reinforced concrete from the point of view of the possibility of its rehabilitation, mainly based on the forecast of the degree of carbonation of different types of concrete structures in various conditions. Based on the results of this analysis, it can be stated that the service life of internal concrete structures can generally be extended up to 100 years, while external concrete elements after 50 years of operation may already require replacement or repair. A comparative analysis of various reconstruction strategies in Ukraine from the point of view of CO2 emissions is also given. It shows that the rehabilitation of such buildings is the most meaningful strategy.