bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunization with a Prefusion SARS-CoV-2 Spike Protein Vaccine (RBMRNA-176) Protects against Viral Challenge in Mice and Nonhuman Primates
Authors: Ma Qinhai , Li Runfeng , Guo Jianmin , Li Man , Ma Lin , Dai Jun , Shi Yongxia , Dai Jinlong , Huang Yuankeng , Dai Cailing , Pan Weiqi , Zhong Huiling , Zhang Hong , Wen Jian , Zhao Haoting , Wu Linping , Yang Wei , Zhang Biliang , Yang Zifeng
Published: October 2022

There is an urgent need for a broad-spectrum and protective vaccine due to the emergence and rapid spreading of more contagious SARS-CoV-2 strains. We report the development of RBMRNA-176, a pseudouridine (Ψ) nucleoside-modified mRNA-LNP vaccine encoding pre-fusion stabilized trimeric SARS-CoV-2 spike protein ectodomain, and evaluate its immunogenicity and protection against virus challenge in mice and nonhuman primates. A prime-boost immunization with RBMRNA-176 at intervals of 21 days resulted in high IgG titers (over 1:819,000 endpoint dilution) and a CD4+ Th1-biased immune response in mice. RBMRNA-176 vaccination induced pseudovirus-neutralizing antibodies with IC50 ranging from 1:1020 to 1:2894 against SARS-CoV-2 spike pseudotyped wild-type and variant viruses, including Alpha, Beta, Gamma, and Kappa. Moreover, significant control of viral replication and histopathology in lungs was observed in vaccinated mice. In nonhuman primates, a boost given by RBMRNA-176 on day 21 after the prime induced a persistent and sustained IgG response. RBMRNA-176 vaccination also protected macaques against upper and lower respiratory tract infection, as well as lung injury. Altogether, these findings support RBMRNA-176 as a vaccine candidate for prevention of COVID-19.
Fear of COVID-19 and Vaccine Hesitancy among Pregnant Women in Poland: A Cross-Sectional Study
Authors: Janik Kinga , Nietupska Kinga , Iwanowicz-Palus Grazyna , Cybulski Mateusz
Published: October 2022

Introduction: Pregnant women are particularly vulnerable to anxiety and stress, and the COVID-19 pandemic has definitely contributed to anxiety in this group. Researchers continue their work on COVID-19 vaccine formulations to reduce the spread of the SARS-CoV-2 virus and minimise the impact of the pandemic. Despite the increased prevalence and severity of anxiety among pregnant women during the COVID-19 pandemic, their attitudes towards COVID-19 vaccine vary. The aim of this study was to assess the levels of anxiety experienced by pregnant women due to COVID-19 and their attitudes to vaccination. Materials and methods: A total of 595 women voluntarily participated in the study. The respondents were divided into two groups: the study group (n = 288), which consisted of women who were pregnant at the time of the survey, and the control group (n = 307), which included women of reproductive age (18–49 years). The study used a diagnostic survey method with a web-based questionnaire consisting of the author’s survey questionnaire and the following standardised tools: the Scale to Measure the Perception of SARS-CoV-2 Vaccines Acceptance (VAC-COVID-19 SCALE), the Fear of COVID-19 Scale (FCV-19S), the Drivers of COVID-19 Vaccination Acceptance Scale (DrVac-COVID19S) and the Coronavirus Anxiety Scale (CAS). Results: The level of COVID-related anxiety differed depending on the tool used. Mild anxiety was reported for CAS, while FCV-19S showed its moderate levels. Both pregnant women and women of reproductive age showed high scores in VAC-COVID-19 and DrVac-COVID19S. The mean VAC-COVID-19 scores were 41.44 in the study group and 44.26 in the control group, and the mean DrVac-COVID19S scores were 51.25 in pregnant women and 55.85 in women of reproductive age. This indicates a high level of acceptance of and positive attitudes toward vaccinations. Conclusions: Pregnant women showed moderate coronavirus anxiety. Women in both the study group and the control group showed mostly positive attitudes towards COVID-19 vaccination.
Safety and Immunogenicity of a Heterologous Booster of Protein Subunit Vaccine MVC-COV1901 after Two Doses of Adenoviral Vector Vaccine AZD1222
Authors: Cheng Shu-Hsing , Lin Yi-Chun , Chen Cheng-Pin , Cheng Chien-Yu
Published: October 2022

We report the safety and immunogenicity results in participants administrated with a booster dose of protein subunit vaccine MVC-COV1901 at 12 (Group A) or 24 (Group B) weeks after two doses of AZD1222 (ChAdOx1 nCoV-19). The administration of the MVC-COV1901 vaccine as a booster dose in both groups was generally safe. There were no serious adverse events related to the intervention as adverse events reported were “mild” or “moderate” in nature. In subjects fully vaccinated with two doses of AZD1222, waning antibody immunity was apparent within six months of the second dose of AZD1222. At one month after the MVC-COV1901 booster dose, those who were vaccinated within 12 weeks after the last AZD1222 dose (Group A) had anti-SARS-CoV-2 spike IgG antibody titers and neutralizing antibody titers which were 14- and 6.5-fold increased, respectively, when compared to the titer levels on the day of the booster dose. On the other hand, fold-increase a month post-booster in people who had a booster 24 weeks after the last AZD1222 dose (Group B) were 19.5 and 14.0 times for anti-SARS-CoV-2 spike IgG antibody titers and neutralizing antibody titers, respectively. Among those who were vaccinated within 12 weeks after the last AZD1222 dose, we also observed 5.2- and 5.6-fold increases in neutralizing titer levels against ancestral strain and Omicron variant pseudovirus after the booster dose, respectively. These results support the use of MVC-COV1901 as a heterologous booster for individuals vaccinated with AZD1222. Furthermore, regardless of the dosing schedule, the combination of AZD1222 primary series and MVC-COV1901 booster can be cost-effective and suitably applied to low- and middle-income countries (LMIC).
Rapid Spread and Genetic Characterisation of a Recently Emerged Recombinant Lumpy Skin Disease Virus in Thailand
Authors: Suwankitwat Nutthakarn , Songkasupa Tapanut , Boonpornprasert Prakit , Sripipattanakul Phurida , Theerawatanasirikul Sirin , Deemagarn Taweewat , Suwannaboon Minta , Arjkumpa Orapun , Buamithup Noppawan , Hongsawat Akkarapol , Jindajang Sirima , Nipaeng Nawakarn , Aunpomma Dilok , Molee Lamul , Puangjinda Kanokwan , Lohlamoh Walaiporn , Nuansrichay Bandit , Narawongsanont Rawint , Arunvipas Pipat , Lekcharoensuk Porntippa
Published: October 2022

The emergence of the lumpy skin disease virus (LSDV) was first detected in north-eastern Thailand in March 2021. Since then, the abrupt increase of LSD cases was observed throughout the country as outbreaks have spread rapidly to 64 out of a total of 77 provinces within four months. Blood, milk, and nodular skin samples collected from affected animals have been diagnosed by real-time PCR targeting the p32 gene. LSDV was isolated by primary lamb testis (PLT) cells, followed by Madin-Darby bovine kidney (MDBK) cells, and confirmed by immunoperoxidase monolayer assay (IPMA). Histopathology and immunohistochemistry (IHC) of a skin lesion showed inclusion bodies in keratinocytes and skin epithelial cells. Phylogenetic analyses of RPO30 and GPCR genes, and the whole genome revealed that Thai viruses were closely related to the vaccine-derived recombinant LSDV strains found previously in China and Vietnam. Recombination analysis confirmed that the Thai LSDV possesses a mosaic hybrid genome containing the vaccine virus DNA as the backbone and a field strain DNA as the minor donor. This is an inclusive report on the disease distributions, complete diagnoses, and genetic characterisation of LSDV during the first wave of LSD outbreaks in Thailand.
Molecular Characterization of CF33 Canine Cell Line and Evaluation of Its Ability to Respond against Infective Stressors in Sight of Anticancer Approaches
Authors: Razzuoli Elisabetta , De Ciucis Chiara Grazia , Chirullo Barbara , Varello Katia , Zoccola Roberto , Guardone Lisa , Petrucci Paola , Rubini Danja , Bozzetta Elena , Goria Maria , Fruscione Floriana , Modesto Paola
Published: October 2022

Spontaneous mammary tumors are the most frequent neoplasms in bitches and show similarities with human breast cancer in risk factors, clinical course, and histopathology. The poor prognosis of some cancer subtypes, both in human and dog, demands more effective therapeutic approaches. A possible strategy is the new anticancer therapy based on immune response modulation through bacteria or their derivatives on canine mammary carcinoma cell lines. The aim of the present study was to analyze the CF33 cell line in terms of basal expression of immune innate genes, CXCR4 expression, and interaction with infectious stressors. Our results highlight that CF33 maintains gene expression parameters typical of mammary cancer, and provides the basal gene expression of CF33, which is characterized by overexpression of CXCR4, CD44, RAD51, LY96, and a non-continuous expression of TP53 and PTEN. No mutations appeared in the CXCR4 gene until the 58th passage; this may represent important information for studying the CXCR4 pathway as a therapeutic target. Moreover, the CF33 cell line was shown to be able to interact with Salmonella Typhimurium (ST) (an infective stressor), indicating that these cells could be used as an in vitro model for developing innovative therapeutic approaches involving bacteria.
Retrospective Study on Risk Factors and Short-Term Outcome of Horses Referred for Colic from 2016 to 2022
Authors: Straticò Paola , Varasano Vincenzo , Palozzo Adriana , Guerri Giulia , Celani Gianluca , Revelant Ortensia , Petrizzi Lucio
Published: October 2022

(1) Background: colic syndrome is a multifactorial life-threatening condition in equids. Awareness of predisposing and prognostic indicators is useful to adequately inform the owner of the outcome and plan the best treatment. The aim of the study was to identify the variables associated with short-term survival in a cohort of horses referred for colic to a veterinary teaching hospital; (2) Methods: medical records of horses referred for colic from 2016 to 2022 were retrospectively reviewed. Univariate and multivariate regression models were built to assess the odds ratios of discharge from the hospital, both on the whole sample and in those patients undergoing surgery; (3) Conclusions: Month, time of admission, cardiovascular variables (heart rate on admission and after 3–6 h, packed cell volume, capillary refill time, and mucosal membranes), and presence of gastric reflux were significantly associated with discharge in the univariate regression in all horses and in those surgically treated. In the multivariate regression model, heart rate, packed cell volume, and capillary refill time showed significant association with the outcome in the whole sample. Although comparison between this study and previous studies is difficult due to the number and type of variables included, cardiovascular variables and markers of hypovolemia were also shown to be associated with outcome.
Changes in Perioperative Antimicrobial and Anti-Inflammatory Drugs Regimens for Colic Surgery in Horses: A Single Center Report
Authors: Gandini Marco , Cerullo Anna , Franci Paolo , Giusto Gessica
Published: October 2022

Reducing postoperative incisional infection is the main reason to administer postoperative antimicrobials (AMD) after emergency laparotomy in horses, while reducing inflammation and providing analgesia are the reasons to administer anti-inflammatory drugs (AID). The basis for postoperative AMD and AID administration is empirical and only recently has been questioned. Empirical approaches can be changed, and these changes, along with the description of their outcomes, can help produce appropriate stewardship. The aim of this study is to report the changes in AMD and AID regimens in horses undergoing emergency laparotomy at a referral teaching hospital between 2017 and 2021. Signalment, pathology, surgery, prophylactic AMD and AID administration were obtained from the medical records. Difference in AMD and AID regimens throughout the study period were also reported. In 234 postoperative records considered, ninety-two horses received prophylactic AMD, while 142 received pre-operative antimicrobials only. There was a progressive change in regimens throughout the years, increasing the number of AID molecules used. AMD and AID administration in horses has changed in our practice over the years to modulate therapies according to the postoperative complications that eventually arise. In this study, horses not receiving postoperative routine AMD treatment did not show an increased incidence of complications.
Feline Oncogenomics: What Do We Know about the Genetics of Cancer in Domestic Cats?
Authors: Ludwig Latasha , Dobromylskyj Melanie , Wood Geoffrey A. , van der Weyden Louise
Published: October 2022

Cancer is a significant cause of morbidity and mortality in domestic cats. In humans, an understanding of the oncogenome of different cancer types has proven critical and is deeply interwoven into all aspects of patient care, including diagnostics, prognostics and treatments through the application of targeted therapies. Investigations into understanding the genetics of feline cancers started with cytogenetics and was then expanded to studies at a gene-specific level, looking for mutations and expression level changes of genes that are commonly mutated in human cancers. Methylation studies have also been performed and together with a recently generated high-quality reference genome for cats, next-generation sequencing studies are starting to deliver results. This review summarises what is currently known of the genetics of both common and rare cancer types in cats, including lymphomas, mammary tumours, squamous cell carcinomas, soft tissue tumours, mast cell tumours, haemangiosarcomas, pulmonary carcinomas, pancreatic carcinomas and osteosarcomas. Shining a spotlight on our current understanding of the feline oncogenome will hopefully serve as a springboard for more much-needed research into the genetics of cancer in domestic cats.
Mott Cell Differentiation in Canine Multicentric B Cell Lymphoma with Cross-Lineage Rearrangement and Lineage Infidelity in a Dog
Authors: Kim Woo-Sub , Song Kun-Ho , Bae Hyeona , Yu DoHyeon , Song Joong-Hyun
Published: October 2022

Lymphoma is a severe condition characterized by the proliferation of neoplastic lymphoid cells. A 4-year-old female mongrel dog presented with solitary lymph node enlargement. Significant right prescapular lymphadenopathy and abdominal enlargement were observed during physical examination. A complete blood count revealed lymphocytosis, and a peripheral blood smear revealed lymphoblastosis and Mott cells. Fine needle aspiration cytology (FNAC) of the right prescapular lymph node revealed a predominant population of lymphoblasts and Mott cells. Based on the FNAC and blood smear results, the patient was diagnosed with leukemic state multicentric B-cell lymphoma with Mott cell differentiation. Subsequent PCR for antigen receptor rearrangement and flow cytometry revealed that the patient exhibited cross-lineage rearrangement (CLRA) and lineage infidelity (LI), respectively. CHOP-based chemotherapy was initiated, however, the patient’s disease was progressive. The patient died three months after the initial presentation. Mott cell differentiation in canine B-cell lymphoma (MCL) has rarely been reported in the veterinary literature and seems to show an unusual clinical course. To the best of our knowledge, no reports of MCL with CLRA and LI exist. We report the clinical features, diagnosis, and treatment of MCL with CLRA and LI.
Construction of Recombinant Rabies Virus Vectors Expressing H or F Protein of Peste des Petits Ruminants Virus
Authors: Wang Haojie , Bi Jinhao , Feng Na , Zhao Yongkun , Wang Tiecheng , Li Yuetao , Yan Feihu , Yang Songtao , Xia Xianzhu
Published: October 2022

Peste des petits ruminants (PPR) is one of the most contagious and fatal diseases of small ruminants in the world and is classified as a category A epidemic disease. It is the target of a global eradication campaign led by the Office International des Epizooties (OIE) and Food and Agriculture Organization of the United Nations (FAO). The PPR live attenuated vaccine is currently the most widely used and approved vaccine, but the use of this vaccine interferes with the serological testing of the PPR elimination program, and there is a potential safety risk. Viral vector vaccines are one of the most promising methods to solve this dilemma. In this study, the full-length infectious clone plasmid of rabies virus (RABV), pD-SRV9-PM-LASV, was used as the backbone, and the envelope glycoprotein H (hemagglutinin protein) or F (fusion protein) gene of PPRV was inserted into the backbone plasmid to construct the infectious clones pD-SRV9-PM-PPRV-H and pD-SRV9-PM-PPRV-F, which express the PPRV H and PPRV F genes, respectively. The correct construction of these infectious clones was verified after sequencing and double digestion. The infectious clones were transfected with a helper plasmid into BSR/T7 cells, and recombinant viruses were successfully rescued by direct immunofluorescence, indirect immunofluorescence, Western blotting, and transmission electron microscopy and named rSRV9-H and rSRV9-F. The results of growth kinetics studies indicated that the inserted gene did not affect virus proliferation. Stability studies revealed that the inserted target gene was stably expressed in recombinant RABV for at least 15 generations. In this study, the recombinant viruses rSRV9-H and rSRV9-F were successfully rescued. The constructed viruses had good proliferative activity and stability and provided potential bivalent inactivated vaccine candidate strains for the prevention of PPR and livestock rabies.
Pituitary Pars Intermedia Dysfunction (PPID) in Horses
Authors: Kirkwood Naomi C. , Hughes Kristopher J. , Stewart Allison J.
Published: October 2022

Substantial morbidity results from pituitary pars intermedia dysfunction (PPID) which is often underestimated by owners and veterinarians. Clinical signs, pathophysiology, diagnostic tests, and treatment protocols of this condition are reviewed. The importance of improved recognition of early clinical signs and diagnosis are highlighted, as initiation of treatment will result in improved quality of life. Future research should be targeted at improving the accuracy of the diagnosis of PPID, as basal adrenocorticotropic hormone (ACTH) concentration can lack sensitivity and thyrotropin releasing hormone (TRH) used to assess ACTH response to TRH stimulation is not commercially available as a sterile registered product in many countries. The relationship between PPID and insulin dysregulation and its association with laminitis, as well as additional management practices and long-term responses to treatment with pergolide also require further investigation.
Dietary Supplementation of Attapulgite Improves Growth Performance in Pigs from Weaning to Slaughter
Authors: Kanoulas Vasileios , Papadopoulos Georgios A. , Tassis Panagiotis , Koutouzidou Georgia , Arsenos Georgios , Fortomaris Paschalis
Published: October 2022

The objective of the present study was to investigate the effect of dietary supplementation of attapulgite on the performance of fattening pigs from weaning to slaughter under field conditions in three commercial farrow to finish herds. In total 1890 pigs were used for six months: 720 pigs in Farms A and B, respectively and 450 pigs in Farm C. The pigs were equally allocated in three dietary treatments: CON, standard diet in each growing phase; ATT, standard diet that was supplemented with attapulgite at 7 kg/tn of feed; and ATT+, standard diet that was supplemented with 8 kg/tn of feed with a compound product based on attapulgite. Pigs that were fed diets that were supplemented with attapulgite (ATT and ATT+) had significantly higher (p < 0.05) average daily feed intake (ADFI) and a better feed conversion ratio (FCR), compared to the control (CON). In conclusion, attapulgite supplementation in the diet of pigs from weaning to slaughter can improve their performance in commercial farms.
Feline Oral Squamous Cell Carcinoma: A Critical Review of Etiologic Factors
Authors: Sequeira Inês , Pires Maria dos Anjos , Leitão José , Henriques Joaquim , Viegas Carlos , Requicha João
Published: October 2022

Feline oral squamous cell carcinoma (FOSCC) is the most common oral neoplasia in cats. This malignant tumor is locally invasive, has a high mortality rate, and its etiology is not yet known. In humans, head and neck squamous cell carcinoma is associated with tobacco smoke, alcohol consumption, and human papillomavirus infection. Herein, a critical review about the potential etiologic factors of FOSCC was performed, considering publications between 2000 and 2022, aiming to synthesize all available scientific evidence regarding this issue. Recommendations of the PRISMA statement and the Cochrane Collaboration were followed and the PubMed database searched by using the MeSH terms MeSH terms “oral”, “mouth”, “lingual”, “labial”, “gingiva”, “carcinoma”, “squamous”, and “feline”. The selection process for eligible studies was based on specific inclusion and exclusion criteria and the quality of the studies assessed. The initial search resulted in 553 publications, with only 26 of these being included in the review. Sixteen studies were related to viral etiology and nine related to environmental factors such as exposure to tobacco smoke, ectoparasitic products, and the presence of oral comorbidities. When evaluated, feline papillomavirus was detected in 16.2% of samples of FOSCC. In the three studies focused on exposure to tobacco smoke, 35.2% (30/85) of cats with FOSCC had a history of this exposure. The consumption of canned food and the use of deworming collars were associated, in only one publication, with a risk of neoplasia increased by 4.7 and 5.3 times, respectively. Among 485 cats with FOSCC, 6.4% had dental and oral pathology (i.e., periodontal disease or feline chronic gingivostomatitis). The present study demonstrates that the available evidence on the etiology of FOSCC is still limited, however, there has been an increasing interest on this topic. To better understand the role of the possible etiological factors of this aggressive disease, and model for its human counterpart, large, prospective multi-institutional studies are needed.
The Influence of Distinct Seasons on the Succession and Diversity of Bacteria on the Anticorrosive Coatings Surfaces in a Marine Environment
Authors: Li Shuangwei , Liu Jie , Li Qian , Li Wenfang , Xiao Xinfeng , Zhang Linlin
Published: October 2022

Epoxy resin has been frequently used as a coating paint for anticorrosion protection because of its excellent chemical properties. However, the long-term succession of bacteria colonizing coatings surfaces in the different seasons of the year remains uncharacterized. In this work, amplicon-based 16s rDNA sequencing was used to characterize the tempol change of bacterial communities growing on the epoxy resin surfaces. The results showed that bacterial diversity indices on spring and autumn immersion samples were higher than that of the samples immersed on summer and winter samples. Proteobacteria was found to be the dominant bacteria of all different seasons and accounted for 57.9% of the total sequence. Gammaproteobacteria and Alphaproteobacteria were the dominant classes in all of the samples, whereas the most abundance bacteria at the genus level had the significant differences with a change of season. Firmicutes also displayed a distinct temporal change pattern in that it was the second abundance in the summer and autumn samples, but had a marked decrease in the other season samples. These results demonstrated that bacterial community composition underwent obvious changes over the distinct seasons of a year. This study will be helpful for the seasonal change of bacterial diversity and development of corrosion-resistant paints.
Impact of Electric Vehicle Charging Synchronization on the Urban Medium Voltage Power Distribution Network of Frederiksberg
Authors: Unterluggauer Tim , Hipolito F. , Klyapovskiy Sergey , Andersen Peter Bach
Published: October 2022

The uptake of electric vehicles (EVs) may pose a challenge to power distribution networks (PDNs). While smart charging can be deployed to relieve stress on the grid, user-centric smart charging strategies could also exacerbate peak power demand due to synchronization when optimizing charging with regard to different objectives, such as charging costs. In this paper, we assess the charging demand emerging from a large fleet of EVs, with models for the decision to charge and distribution of the steady-state state-of-charge (SoC). These are applied to the municipality of Frederiksberg, Denmark, using data from the Danish national travel survey. Home and workplace charging are mapped to the urban 10 kV medium voltage PDN of Frederiksberg considering different charging behaviors and degrees of synchronization. Results indicate that the likelihood of severe congestion in the power distribution network is low and that it can be attributed to rare scenarios in which high synchronization is observed, particularly when maintaining the normal steady-state demand. Despite the low likelihood, preventive measures should be devised to mitigate such scenarios, especially if additional high-power consumers are connected.
Empirical Analysis of the User Needs and the Business Models in the Norwegian Charging Infrastructure Ecosystem
Authors: Figenbaum Erik , Wangsness Paal Brevik , Amundsen Astrid Helene , Milch Vibeke
Published: October 2022

The Norwegian charging infrastructure ecosystem was investigated from a user perspective by (1) developing knowledge of end-user experiences with public charging, (2) mapping BEV owners and future owner’s user-friendliness needs and the extent to which these needs are met, (3) pointing at potential user-friendliness improvements, (4) mapping the charging infrastructure ecosystem and business models, and (5) developing scenarios for the future system development and the impact on charging infrastructure user-friendliness. The article draws on the literature, a BEV (battery electric vehicle) and ICEV (internal combustion engine vehicle) owner survey, 15 BEV owner interviews, 21 charging infrastructure actor interviews, and open information sources on the charger actors. The unregulated charging system evolved into a complex web of actors that developed their own charging networks following their individually sensible business models, which in sum led to serious user-friendliness issues. To gain access to all chargers, users need to interact with up to 20–30 apps and 13 payment systems, which comes on top of different plug types, power levels, and charger interfaces. Some actors support roaming, while others oppose it. OEMs want users to interface with chargers through the navigation system. In the future, the system will become even more complex and less user friendly as more actors join unless, e.g., consolidation, regulation, or independent network orchestrators reduce the complexity.
Aquila Optimization Algorithm for Wind Energy Potential Assessment Relying on Weibull Parameters Estimation
Authors: Abou El-Ela Adel A. , El-Sehiemy Ragab A. , Shaheen Abdullah M. , Shalaby Ayman S.
Published: October 2022

Statistical distribution approaches have been developed to describe wind data due to the intermittent and unpredictable nature of wind speed. The Weibull distribution with two parameters is thought to be the most accurate distribution for modeling wind data. This study seeks wind energy assessment via searching for the optimal estimation of the Weibull parameters. For this target, analytical and heuristic methods are investigated. The analytical methods involve the maximum likelihood, moment, energy pattern factor, and empirical methods, while the heuristic optimization algorithms include particle warm optimization and the Aquila optimizer (AO). Both analytical and heuristic methods are assessed together to fit the probability density function of wind data. In addition, nine models are submitted to find the most appropriate model to represent wind energy production. The error between actual and estimated wind energy density is computed to the model for each study site which has less error of energy. The fit test is performed with real data for the Zafarana and Shark El-Ouinate sites in Egypt for a year. Additionally, different indicators of fitness properties are assessed, such as the root mean square error, determination coefficient (R2), mean absolute error, and wind production deviation. The simulation results declare that the proposed AO optimization algorithm offers greater accuracy than several optimization algorithms in the literature for estimating the Weibull parameters. Furthermore, statistical analysis of the compared methods demonstrates the high stability of the AO algorithm. Thus, the proposed AO has greater accuracy and more stability in the obtained outcomes for Weibull parameters and wind energy calculations.
Discussing EU Policies and Mechanisms towards the COVID-19 Pandemic Crisis: A Case Study of Greece
Authors: Apostolopoulos Nikolaos , Psychalis Marios , Liargovas Panagiotis
Published: October 2022

This paper discusses the effectiveness of EU economic measures towards the pandemic crisis in the case of Greece. As the pandemic crisis was an exogenous and symmetric crisis, EU member states decided to take supply and demand side measures to tackle economic recession. Not only the Recovery plan for Europe (NGEU), but also the Escape Clause, as well as non-standard monetary measures, were implemented in order to achieve growth. Furthermore, fiscal expansion, as well as common debt extraction, using green and social bonds led to higher government spending and sovereign debt. The paper’s research question is “Could fiscal expansion mitigate the economic consequences of pandemic crisis?” In other worlds, the research gap which this paper tries to fill is that for the contemporary EU response to two different crises, the economic and the pandemic. Our analysis, by using a comparative approach, shows that government spending and fiscal expansion is effective in the short-run, as the temporary measures led to higher GDP growth rates and lower unemployment rates, but in the long-run demand side measures led to higher inflation and higher sovereign debt.
THE IMPACT OF PROFESSIONAL SKILLS IN THE USE OF INFORMATION AND COMMUNICATION TECHNOLOGIES ON THE INCOME FROM EMPLOYMENT: GENERATIONAL DIFFERENTIATION
Authors: Bobkov Vyacheslav N. , Odintsova Elena V. , Shichkin Igor A.
Published: October 2022

The article presents the results of a research aimed at identifying the level of proficiency in the use of ICT tools (ICT skill levels) among employed and unemployed and also generational groups (youth, middle and older generation), as well as identifying the quality of employment and income depending on the level of ICT skills of employed and their generational groups. The author’s classification of the ICT skills levels is presented and, on its basis, a quantitative identification of the ICT skills levels among the employed and unemployed is carried out. It was found that among the employed, 40.5% have the ICT skills required for work, including 1.8% are distinguished by the presence of specialized skills corresponding to the third (highest) level, the remaining 59.5% have basic ICT skills. Among the unemployed, compared to the employed, the share of those with only basic ICT skills not related to professional activity (75.1%) is significantly higher, while the share of those with the third (highest) (0.9%) and second (24.0%) the level of ICT skills, on the contrary, is lower relative to the employed. Generational differences in the levels of ICT skills among employed and unemployed were revealed. Among the youth, the proportion of those who possess the highest ICT skills and have realized their labor potential is higher than for the employed as a whole and their other generational groups. Estimates of the distribution of employed and their generational groups according to the level of income from the main employment, determined on the basis of original system of social standards, depending on the level of ICT skills, are obtained. As a result of the research, it was revealed that a higher level of ICT skills increases the chances of realizing the existing labour potential. Meanwhile, as higher the level of ICT skills, the greater chances of “converting” them into higher incomes due to employment. It is shown that implementation of labour potential in the labour market with a higher level of ICT skills determines lower risks of reducing the quality of employment, and also increases the chances for employed people to ensure higher standards of material prosperity in their households.
First case of chronic systemic spironucleosis in Freshwater Angelfish (Pterophyllum scalare Schultze, 1823) in Türkiye
Authors: YARDIMCI Remziye Eda , TURGAY Emre , KARATAŞ STEINUM Süheyla
Published: October 2022

This study aimed to identify the cause of sustained mortality in freshwater angelfish (Pterophyllum scalare Schultze, 1823) kept at an ornamental fish production facility in Türkiye. Parasitological, bacteriological and histopathological examination were performed on moribund hybrid angelfish individuals. The moribund fish had haemorrhaged eyes, darkened skin, scale loss, ascites and false faeces. A pale liver, splenomegaly and a thinning of the intestinal wall were observed internally. The parasitological examination revealed flagellated protozoan endoparasite Spironucleus sp. in the intestine. A number of histopathological changes were observed including lipid degeneration in the liver, hemosiderin deposits as well as granulomas in the spleen, a large number of mast cells in the lamina propria of the intestine and enteritis. Numerous Spironucleus parasites were seen in the intestinal wall. According to physiological and biochemical tests, the bacterial isolates obtained from the visceral organs of some fish were identified as Citrobacter freundii. After oral metronidazole treatment, with a dose of 50 mg/kg fish daily for 5 days, a decrease in fish mortality and resumed feeding were noted. A chronic spironucleosis, which systemically affects fish by penetrating the intestinal mucosa, was identified as the cause of this sustained mortality in freshwater angelfish.
Induction of Triploidy by Cold Shock in Indian catfish, Heteropneustes fossilis (Bloch)
Authors: TIWARY B.K.
Published: October 2022

Indian catfish (Heteropneustes fossilis, Heteropneustidae) is a highly nutritious fish with a comparatively slow growth rate compared to other popular Indian fishes. Triploids are expected to show higher growth potential due to sterility and reduced gonadal develop­ment. In an attempt to improve growth potential in this catfish, activated eggs were sub­jected to cold shock at 4 °C for different trial times (10, 20, 30 and 40 minutes) starting at 2 minutes post-fertilization. The optimum duration for cold shock was found to be 30 minutes. Triploids (3n=84) were distinguished from diploids (2n=56) by micromeasurements of erythrocytes and chromosome counts.
Pituitary Dose Optimization for Induced Ovulation, In Vitro Fertilization and Production of Normal Fry of Clarias batrachus (Linn.)
Authors: GOSWAMI U.C. , SARMA N.N.
Published: October 2022

An effective dose optimization with varying doses of carp pituitary (Labeo rohita) extract injection (10-60 mg· kg·1) was attempted in order to standardize the carp pituitary dose for induction of ovulation and in vitro fertilization of stripped eggs of the catfish Clarias batrachus. Fish of body weight 140-158 g were selected. Female catfish were in­jected with pituitary extract; after 17 h latency, the eggs were stripped and fertilized with testicular homogenate taken from minced testes of males in normal saline solution. Males were not injected with pituitary extract. Hatching occurred after 30 h incubation at 27-28 °C in a plastic bucket with water flow system. The 40 mg• kg-1 dose of carp pituitary extract resulted in the production of 87% normal fry, which corresponds to 4,768 fry per 100 g body weight of the fish. Doses of 50-60 mg·kg·1 pituitary extract gave similar results.
Hydrolysis of Proteins from Liza subviridis
Authors: YU S.Y. , AHMAD R.
Published: October 2022

Proteins from Liza subviridis, a marine fish, were hydrolyzed using Alcalase 2.5L. The parameters studied were pH, temperature, ratio of enzyme to substrate concentration, and substrate concentration. It was observed that the optimum conditions for the hydrolysis were pH 9 at 40°C, using an enzyme to substrate ratio of 16 Au/kg and a substrate concentration of 5%.
The Effect of Egg Density on Hatch Rate of Pearl Oyster (Pinctada maxima and P. margaritifera) Larvae
Authors: SOUTHGATE P.C.
Published: October 2022

This paper reports on two experiments in which the effect of egg density on hatch rate of pearl oyster Pinctada maxima (Jameson) and P. margaritifera (L.) larvae was determined. Fertilized eggs were stocked at densities of 10, 20, 30, 40, 50 and 100 ml· 1 for P. maxima and 10, 20, 30, 50, 100 and 150 mJ-1 for P. margaritifera. After 24 h, the number of D-stage veliger larvae was assessed and survival was calculated. Highest survival was shown at an egg density of 10 mt-1 for both P. maxima (82.5%) and P. margaritifera (93.2%). Lowest survival was shown at a density of 100 ml-1 for both P. maxima (74.1%) and P. margaritifera (79.4%). However, for both species, there was no significant difference in survival between densities of 20 and 50 eggs mi-1 or between 30 and 100 eggs ml-1.
Insights into Photothermally Enhanced Photocatalytic U(VI) Extraction by a Step-Scheme Heterojunction
Authors: Zhang Yifeng , Sun Haorong , Gao Feixue , Zhang Shuo , Han Qingzhi , Li Jing , Fang Ming , Cai Yawen , Hu Baowei , Tan Xiaoli , Wang Xiangke
Published: October 2022

In this work, a CdS/BiVO
4
step-scheme (S-scheme) heterojunction with self-photothermally enhanced photocatalytic effect was synthesized and applied for efficient U(VI) photoextraction. Characterizations such as transient absorption spectroscopy and Tafel test together confirmed the formation of S-scheme heterojunctions, which allows CdS/BiVO
4
to avoid photocorrosion while retaining the strong reducing capacity of CdS and the oxidizing capacity of BiVO
4
. Experimental results such as radical quenching experiments and electron spin resonance show that U(VI) is rapidly oxidized by photoholes/
•
OH to insoluble UO
2
(OH)
2
after being reduced to U(IV) by photoelectrons/
•
O
2
-
, which precisely avoids the depletion of electron sacrificial agents. The rapid recombination of electron-hole pairs triggered by the S-scheme heterojunction is found to release large amounts of heat and accelerate the photocatalysis. This work offers a new enhanced strategy for photocatalytic uranium extraction and presents a direction for the design and development of new photocatalysts.
Dueñas de la tierra: representaciones e imaginarios construidos en relación a las herederas de Estancia Tuyutí, Córdoba, 1912-1966
Authors: Roizarena Sofia
Published: October 2022

El presente artículo es un recorrido biográfico alrededor de la trayectoria de dos mujeres, quienes gerenciaron yadministraron por más de cincuenta años una estancia ganadera en el sur cordobés. Nuestro objetivo es poder reflexionar acerca de las diferentes estrategias a las cuales apelaron para poder sobreponerse a la norma de la época y trascender el espacio doméstico. A la vez, analizaremos las diferentes representaciones que de ellas se fueron imprimiendo en el imaginario colectivo.
Entre lógicas estatales de gestión y demandas indígenas de clasificación
Authors: Sabio Collado María Victoria , Milana María Paula
Published: October 2022

Este artículo analiza el proceso de reconocimiento formal de los pueblos diaguita y kolla en la Provincia de Salta, cuyas pertenencias indígenas no fueron consideradas por los organismos indigenistas locales sino hasta principios del siglo XXI. Para ello aborda un conjunto de políticas estatales de orden censal y legislativo, en contrapunto con agencias involucradas en procesos organizativos indígenas, tanto en los Valles Calchaquíes como en los Valles Interandinos, en un lapso de dos décadas (1983-2003). Se detiene en un evento de 2003 que habilita la incorporación de representantes de ambos pueblos al Instituto Provincial de Pueblos Indígenas de Salta, marcando una inflexión tanto en las luchas colectivas como en la misma lógica de clasificación y administración de las alteridades del Estado provincial.
De los dominios del rey a la ciudad sin territorio. Usos políticos del espacio en el Cabildo de Buenos Aires, 1806-1811.
Authors: Magi Valentín
Published: October 2022

Este artículo indaga sobre la dimensión espacial de lapolítica rioplatense durante las invasiones inglesas y el juntismorevolucionario desde la perspectiva del Cabildo de Buenos Aires.Por un lado, se conceptualiza al espacio concebido en el ocasodel período colonial, y, por otro, se señala su continuidaden tiempos revolucionarios, aunque en aparente contradiccióncon la crisis de la monarquía, a la que se propone pensar entérminos de desterritorialización. En los entresijos de ese pasaje,se sugieren reflexiones sobre fragmentos de las trayectorias dediferentes actores políticos, reparando en las claves espaciales de sulegitimidad. Finalmente, se despliegan las intervenciones del ordenrevolucionario sobre la ciudad de Buenos Aires, a través de losplanos diplomático, festivo y sociopolítico para la configuraciónde un nuevo espacio público y social.
Entre la crisis de la “cultura del código” y la emergencia del derecho laboral: el trabajador rural en el proyecto bonaerense de Código del trabajo (1932-1935)
Authors: Barandiarán Luciano
Published: October 2022

En la primera mitad de la década de 1930 el gobiernode la provincia de Buenos Aires presentó un proyecto de Códigolaboral con el objetivo de unificar el derecho obrero. Su realizaciónhabía sido solicitada por el gobernador Federico Martínez deHoz en 1932 al Departamento del Trabajo de la Provincia(DPT). Aunque finalmente el Código no se sancionó, este artículoanaliza el rol y las funciones que se le adjudicaban al Estadoprovincial para aplicar y vigilar su cumplimiento; también estudialas principales características del mismo, comparándolo con otrasfuentes oficiales que permiten dar cuenta de su itinerario. Perofundamentalmente pretende analizar las vías que allí se pensaronpara beneficiar al obrero rural, muchas de las cuales se oponían a lanormativa vigente anterior a 1940.
Presentación: Desigualdades sociales y mundos del trabajo. Miradas extracéntricas de una realidad plural.
Authors: Cerdá Juan Manuel , Remedi Fernando J.
Published: October 2022

Esta contribución colectiva aborda la problemática delas desigualdades del mundo del trabajo en distintos espacios delinterior de la Argentina en la primera mitad del siglo XX a partir decuatro estudios de caso. Se pretende dar cuenta de las diversidadesde las experiencias laborales y de los procesos de construcciónde la legislación del trabajo desde una perspectiva que involucramúltiples actores sociales y estatales. La perspectiva “descentrada”de las grandes ciudades -en especial Buenos Aires- y focalizada enuna mirada de las desigualdades sociales hacen más complejas ymatizadas las visiones canónicas sobre el mundo del trabajo y sobrela construcción de la legislación laboral.
KONVERSI KAS DAN DAMPAKNYA TERHADAP LIKUIDITAS DAN PROFITABILITAS DEBITUR USAHA KECIL (Studi Kasus di Bank Sinar Harapan Bali)
Authors: Kusumastuti Risa
Published: October 2022

Micro, small, and medium enterprises (SMEs) is an important part of national economy.Despite their advantages, SMEs still often have problems in developing their business and have limited access to capital. In this paper, the author examines the relationship between working capital loan and industry to activity, liquidity, and profitability aspects of small business debtors in Bank Sinar Harapan Bali. The results of this study show that working capital loan and industry influence cash conversion cycle significantly, both simultaneously and partially. Cash conversion cycle affects liquidity and profitability. The total contribution of working capital loan to current ratio through cash conversion cycle variable is amounted 71,05%. The total contribution of industry to current ratio through cash conversion cycle is -14,51%. The total contribution of working capital loan to return on equity through cash conversion cycle variable is amounted 60,48%, while the total contribution of industry to current ratio through cash conversion cycle is -12,35%.
ANALISIS KUALITAS LAYANAN SERVICE TERHADAP TINGKAT KEPUASAN PENGGUNA MOTOR SUZUKI PADA BENGKEL PT SINAR GALESONG PRATAMA MAKASSAR
Authors: Tahendrika Abner
Published: October 2022

The objective of this research is to analyze the impact of service quality, including, tangible, reliability, responsiveness, assurance and empathy toward customer satisfaction at bengkel PT Sinar Galesong Pratama Makassar. This research used quantative research design. Multiple linear regression analysis used in this reserach. The results show that the service quality significantly influences to customer satisfaction and the variables of service quality which have the most influence on customer satisfaction are tangible variable.
Keputusan Penggunaan Layanan Go-Jek di DKI Jakarta Berdasarkan Pengaruh Service Marketing Mix
Authors: Anau Andykha Pratama , Bagasworo Wasi
Published: October 2022

Community these days believe in efficiency and simplicity to access a mobile application, especially for purchasing a certain product. This indicates a wider opportunity for companies to extend their consumers by using the internet. Go-Jek, as a pioneer of mobile application, links ‘ojek’ drivers to deliver a one-stop-shop convenience service for Indonesian citizen. It answers these huge opportunities by expanding its services including transportation, instant courier, GO-FOOD and shopping using two wheels driver. The main purposeof this research is to measure the influence of service marketing mix through purchasing decision in DKI Jakarta - study on Go-Jek user. Data were collected by the writer based on Go-Jek customers’ respondents especially in DKI Jakarta. Quantitative analysis was applied to understand and to measure the hypothesis intention in this research. The result indicates the significant influences between the product, prices, places, promotions, people, process, and physical evidence on purchasing decision. Hence, physical evidence is the most significant variable for a customer in DKI Jakarta toward its purchasing decision. Go-Jek motorbike supported by its proper condition, its completeness riding tools, its cleanliness, its conveniences during riding. They provided a well-kept helmet and several types of equipment for the customers. In addition, Go-Jek drivers maintain their own tidiest and appearances. These are the variable indicators which influence the customer decision for using GoJek services. The results suggested Go-Jek maintaining their supervision and controlling the standard operation for delivering its services to its customers, and punctuality toward responding to the order of the customers