bluuebunny/crossref_metadata_2025 huggingface dataset:
LPS Induces Occludin Dysregulation in Cerebral Microvascular Endothelial Cells via MAPK Signaling and Augmenting MMP-2 Levels
Authors: Qin Lan-hui , Huang Wen , Mo Xue-an , Chen Yan-lan , Wu Xiang-hong
Published: July 2015

Disrupted blood-brain barrier (BBB) integrity contributes to cerebral edema during central nervous system infection. The current study explored the mechanism of lipopolysaccharide- (LPS-) induced dysregulation of tight junction (TJ) proteins. Human cerebral microvascular endothelial cells (hCMEC/D3) were exposed to LPS, SB203580 (p38MAPK inhibitor), or SP600125 (JNK inhibitor), and cell vitality was determined by MTT assay. The proteins expressions of p38MAPK, JNK, and TJs (occludin and zonula occludens- (ZO-) 1) were determined by western blot. The mRNA levels of TJ components and MMP-2 were measured with quantitative real-time polymerase chain reaction (qRT-PCR), and MMP-2 protein levels were determined by enzyme-linked immunosorbent assay (ELISA). LPS, SB203580, and SP600125 under respective concentrations of 10, 7.69, or 0.22 µg/mL had no effects on cell vitality. Treatment with LPS decreased mRNA and protein levels of occludin and ZO-1 and enhanced p38MAPK and JNK phosphorylation and MMP-2 expression. These effects were attenuated by pretreatment with SB203580 or SP600125, but not in ZO-1 expression. Both doxycycline hyclate (a total MMP inhibitor) and SB-3CT (a specific MMP-2 inhibitor) partially attenuated the LPS-induced downregulation of occludin. These data suggest that MMP-2 overexpression and p38MAPK/JNK pathways are involved in the LPS-mediated alterations of occludin in hCMEC/D3; however, ZO-1 levels are not influenced by p38MAPK/JNK.
Lower Urinary Tract Symptoms Are Associated with Increased Risk of Dementia among the Elderly: A Nationwide Study
Authors: Chiang Chi-Hsiang , Wu Ming-Ping , Ho Chung-Han , Weng Shih-Feng , Huang Chien-Cheng , Hsieh Wan-Ting , Hsu Ya-Wen , Chen Ping-Jen
Published: July 2015

Studies show a strong association between dementia and lower urinary tract symptoms (LUTS). The aim of this study was to investigate whether LUTS are a risk factor for cognitive impairment. We enrolled 50-year-old and older subjects with LUTS (LUTS[+]) (n=6801) and controls without LUTS (LUTS[−]) (n=20,403) from Taiwan’s National Health Insurance Research Database. LUTS, dementia, and other confounding factors are defined by International Classification of Diseases, Ninth Revision, Clinical Modification Codes. Participants were recruited from 2000 to 2004 and then followed up until death or the end of 2011. The outcome was the onset of dementia, which was assessed using Poisson regression analysis, Cox hazards models, and Kaplan-Meier survival curves. The incidence of dementia was significantly higher in the LUTS[+]group than in the LUTS[−]group (124.76 versus 77.59/1000 person-years). The increased risk of dementia related to LUTS remained significant after adjustment for potential confounders (adjusted hazard ratio (AHR): 1.61, 95% confidence interval (CI) 1.47–1.76,P<0.0001) and higher than that related to cerebrovascular disease (AHR: 1.43, 95% CI 1.26–1.61,P<0.0001). The outcome suggests the need for early screening and appropriate intervention to help prevent cognitive impairment of patients with LUTS.
The Anti-Inflammatory and Antibacterial Action of Nanocrystalline Silver and Manuka Honey on the Molecular Alternation of Diabetic Foot Ulcer: A Comprehensive Literature Review
Authors: Tsang Ka-Kit , Kwong Enid Wai-Yung , Woo Kevin Y. , To Tony Shing-Shun , Chung Joanne Wai-Yee , Wong Thomas Kwok-Shing
Published: July 2015

Honey and silver have been used since ancient times for treating wounds. Their widespread clinical application has attracted attention in light of the increasing prevalence of antibiotic-resistant bacteria. While there have been a number of studies exploring the anti-inflammatory and antibacterial effects of manuka honey and nanocrystalline silver, their advantages and limitations with regard to the treatment of chronic wounds remain a subject of debate. The aim of this paper is to examine the evidence on the use of nanocrystalline silver and manuka honey for treating diabetic foot ulcers through a critical and comprehensive review of in vitro studies, animal studies, and in vivo studies. The findings from the in vitro and animal studies suggest that both agents have effective antibacterial actions. Their anti-inflammatory action and related impact on wound healing are unclear. Besides, there is no evidence to suggest that any topical agent is more effective for use in treating diabetic foot ulcer. Overall, high-quality, clinical human studies supported by findings from the molecular science on the use of manuka honey or nanocrystalline silver are lacking. There is a need for rigorously designed human clinical studies on the subject to fill this knowledge gap and guide clinical practice.
UGC 7639: A Dwarf Galaxy in the Canes Venatici I Cloud
Authors: Buson L. M. , Bettoni D. , Mazzei P. , Galletta G.
Published: July 2015

We want to get insight into the formation mechanism and the evolution of UGC 7639, a dwarf galaxy in the Canes Venatici I Cloud (CVnIC). We used archival multiwavelength data to constrain its global properties. Ultraviolet images show that UGC 7639 inner regions are composed mostly by young stellar populations. In addition, we used smoothed particle hydrodynamics simulations with chemophotometric implementation to account for its formation and evolution. UGC 7639 is an example of blue dwarf galaxy whose global properties are well matched by our multiwavelength approach, that is, a suitable approach to highlight the evolution also of these galaxies as a class. We found that the global properties of UGC 7639, namely, its total absolute B-band magnitude, its whole spectral energy distribution, and morphology, are well matched by an encounter with a system four times more massive than our target. Moreover, the current star formation rate of the simulated dwarf, ≈0.03 M⊙ yr−1, is in good agreement with our UV-based estimate. We derived a galaxy age of 8.6 Gyr. Following our simulation, the ongoing star formation will extinguish within 1.6 Gyr, thus leaving a red dwarf galaxy.
Transdermal Nicotine Application Attenuates Cardiac Dysfunction after Severe Thermal Injury
Authors: Claassen Leif , Papst Stephan , Reimers Kerstin , Stukenborg-Colsman Christina , Steinstraesser Lars , Vogt Peter M. , Kraft Theresia , Niederbichler Andreas D.
Published: July 2015

Background. Severe burn trauma leads to an immediate and strong inflammatory response inciting cardiac dysfunction that is associated with high morbidity and mortality. The aim of this study was to determine whether transdermal application of nicotine could influence the burn-induced cardiac dysfunction via its known immunomodulatory effects.Material and Methods. A standardized rat burn model was used in 35 male Sprague Dawley rats. The experimental animals were divided into a control group, a burn trauma group, a burn trauma group with additional nicotine treatment, and a sham group with five experimental animals per group. The latter two groups received nicotine administration. Using microtip catheterization, functional parameters of the heart were assessed 12 or 24 hours after infliction of burn trauma.Results. Burn trauma led to significantly decreased blood pressure (BP) values whereas nicotine administration normalized BP. As expected, burn trauma also induced a significant deterioration of myocardial contractility and relaxation parameters. After application of nicotine these adverse effects were attenuated.Conclusion. The present study showed that transdermal nicotine administration has normalizing effects on burn-induced myocardial dysfunction parameters. Further research is warranted to gain insight in molecular mechanisms and pathways and to evaluate potential treatment options in humans.
Huqi San-Evoked Rat Colonic Anion Secretion through Increasing CFTR Expression
Authors: Xue Xiaowei , Shi Zhengming , Wang Wen , Yu Xiaotong , Feng Ping , Zhang Min , Wang Xuejiang , Xu Jingdong
Published: July 2015

Huqi San (HQS) is a Chinese herbal preparation of eight medicinal herbs that promote diuresis, detoxification, blood circulation, and cholestasis. Defects in transporter expression and function can cause cholestasis and jaundice. However, the mechanism of the cholestasis underlying HQS effects, especially on the gastrointestinal tract ion secretion, has not been elucidated. Real-time RT-PCR and Western blotting were used to study the expression and localization of cystic fibrosis transmembrane conductance regulator (CFTR) andα-ENaC in rat alimentary tract, and then the effect of HQS on the ion transport in rat distal colon mucosa was investigated using the short-circuit current (ISC) technique. The results showed that pretreatment with HQS significantly enhanced mRNA transcripts and protein content of CFTR in liver and distal colon but notα-ENaC in alimentary organs. HQS increasesISCand decreases the transepithelial resistance. Pretreatment with epithelial Na+channel blocker did not affect theISCresponses elicited by HQS, but removal of extracellular Cl−or pretreatment with Cl−channel or Na+-K+-2Cl−cotransporter blocker inhibited HQS-elicitedISCresponses. These findings demonstrated that HQS, RA, and RP can stimulate Cl−secretion in the distal colon by increasing the mRNA transcripts and protein content of CFTR in liver and distal colon.
A Novel Model for Lattice-Based Authorized Searchable Encryption with Special Keyword
Authors: Zeng Fugeng , Xu Chunxiang
Published: July 2015

Data stored in the cloud servers, keyword search, and access controls are two important capabilities which should be supported. Public-keyword encryption with keyword search (PEKS) and attribute based encryption (ABE) are corresponding solutions. Meanwhile, as we step into postquantum era, pairing related assumption is fragile. Lattice is an ideal choice for building secure encryption scheme against quantum attack. Based on this, we propose the first mathematical model for lattice-based authorized searchable encryption. Data owners can sort the ciphertext by specific keywords such as time; data users satisfying the access control hand the trapdoor generated with the keyword to the cloud sever; the cloud sever sends back the corresponding ciphertext. The security of our schemes is based on the worst-case hardness on lattices, called learning with errors (LWE) assumption. In addition, our scheme achieves attribute-hiding, which could protect the sensitive information of data user.
Raman Spectroscopy and Electrochemical Investigations of Pt Electrocatalyst Supported on Carbon Prepared through Plasma Pyrolysis of Natural Gas
Authors: Evangelista Tereza Cristina Santos , Paganoto Giordano Toscano , Guimarães Marco Cesar Cunegundes , Ribeiro Josimar
Published: July 2015

Physicochemical and electrochemical characterisations of Pt-based electrocatalysts supported on carbon (Vulcan carbon, C1, and carbon produced by plasma pyrolysis of natural gas, C2) toward ethanol electrooxidation were investigated. The Pt20/C180and Pt20/C280electrocatalysts were prepared by thermal decomposition of polymeric precursors at 350°C. The electrochemical and physicochemical characterisations of the electrocatalysts were performed by means of X-ray diffraction (XRD), transmission electron microscope (TEM), Raman scattering, cyclic voltammetry, and chronoamperometry tests. The XRD results show that the Pt-based electrocatalysts present platinum metallic which is face-centered cubic structure. The results indicate that the Pt20/C180electrocatalyst has a smaller particle size (10.1–6.9 nm) compared with the Pt20/C280electrocatalyst; however, the Pt20/C280particle sizes are similar (12.8–10.4 nm) and almost independent of the reflection planes, which suggests that the Pt crystallites grow with a radial shape. Raman results reveal that both Vulcan carbon and plasma carbon are graphite-like materials consisting mostly of sp2carbon. Cyclic voltammetry and chronoamperometry data obtained in this study indicate that the deposition of Pt on plasma carbon increases its electrocatalytic activity toward ethanol oxidation reaction.
Landing Impact Analysis of a Bioinspired Intermittent Hopping Robot with Consideration of Friction
Authors: Bai Long , Ge Wenjie , Chen Xiaohong , Tang Qian , Xiang Rong
Published: July 2015

Multibody dynamics for the flying and landing oblique impact processes of a bioinspired intermittent hopping robot is derived in this paper by using the impulse-momentum principle. The dynamics model that involves the multibody configuration, mass distribution of the robot, and friction is solved by the linear complementarity conditions in terms of different impact types. The computational and experimental data is compared. And the influence factors of landing impact are analysed as well. Based on the influence rules for landing impact, a technical design of solution is proposed for adjusting the robot’s attitude during the jumping and for absorbing the impact energy during the landing. Lessons learned from the theoretical and experimental results have general applicability to the motion prediction, performance analysis, and landing stability study for intermittent hopping robots or other legged robots.
Papillary Thyroid Cancer and Lung Adenocarcinoma Presenting as Two Primary Malignancies in a Patient with Symptomatic Goiter
Authors: Daniel Deepu , Delumpa Leah , Bray Natasha
Published: July 2015

In rare instances, patients may be diagnosed with two different primary malignancies. Though such synchronous malignancies have been documented in sporadic case reports, the overwhelming majority of malignancies involving multiple organs can be attributed to a primary source. Papillary thyroid carcinoma and lung adenocarcinoma are rarely diagnosed within the same year. Our case report presents a patient who was diagnosed with these two malignancies during her same hospital visit. Biopsies results proved that the two malignancies were in fact separate entities and not a consequence of metastasis from a primary source.
Brodie’s Abscess in a Patient Presenting with Sickle Cell Vasoocclusive Crisis
Authors: Ogbonna Onyekachi Henry , Paul Yonette , Nabhani Hasan , Medina Adriana
Published: July 2015

First described by Sir Nicholas Brodie in 1832, Brodie’s abscess is a localized subacute or chronic infection of the bone, typically seen in the metaphases of long bones in children and adolescents. The diagnosis can prove to be enigmatic due to absence of clinical signs and symptoms of systemic disease. We report a very interesting case of Brodie’s abscess masquerading as sickle cell vasoocclusive crisis in a 20-year-old female with sickle cell disease and review the literature.
Hybrid Motion Planning Method for Autonomous Robots Using Kinect Based Sensor Fusion and Virtual Plane Approach in Dynamic Environments
Authors: Tuvshinjargal Doopalam , Dorj Byambaa , Lee Deok Jin
Published: July 2015

A new reactive motion planning method for an autonomous vehicle in dynamic environments is proposed. The new dynamic motion planning method combines a virtual plane based reactive motion planning technique with a sensor fusion based obstacle detection approach, which results in improving robustness and autonomy of vehicle navigation within unpredictable dynamic environments. The key feature of the new reactive motion planning method is based on a local observer in the virtual plane which allows the effective transformation of complex dynamic planning problems into simple stationary in the virtual plane. In addition, a sensor fusion based obstacle detection technique provides the pose estimation of moving obstacles by using a Kinect sensor and a sonar sensor, which helps to improve the accuracy and robustness of the reactive motion planning approach in uncertain dynamic environments. The performance of the proposed method was demonstrated through not only simulation studies but also field experiments using multiple moving obstacles even in hostile environments where conventional method failed.
The Effect of Spatial Working Memory Deterioration on Strategic Visuomotor Learning across Aging
Authors: Uresti-Cabrera Luis A. , Diaz Rosalinda , Vaca-Palomares Israel , Fernandez-Ruiz Juan
Published: July 2015

Objective. To evaluate the effect of age-related cognitive changes in a visuomotor learning task that depends on strategic control and contrast it with the effect in a task principally depending on visuomotor recalibration.Methods. Participants performed a ball throwing task while donning either a reversing dove prism or a displacement wedge prism, which mainly depend on strategic control or visuomotor recalibration, respectively. Visuomotor performance was then analysed in relation to rule acquisition and reversal, recognition memory, visual memory, spatial planning, and spatial working memory with tasks from the Cambridge Neuropsychological Test Automated Battery (CANTAB).Results. The results confirmed previous works showing a detrimental effect of age on visuomotor learning. The analyses of the cognitive changes observed across age showed that both strategic control and visuomotor recalibration had significant negative correlations only with the number of errors in the spatial working memory task. However, when the effect of aging was controlled, the only significant correlation remaining was between the reversal adaptation magnitude and spatial working memory.Discussion. These results suggest that spatial working memory decline across aging could contribute to age-dependent deterioration in both visuomotor learning processes. However, spatial working memory integrity seems to affect strategic learning decline even after controlling for aging.
Nonlinear Dynamic Characteristics and Optimal Control of SMA Composite Wings Subjected to Stochastic Excitation
Authors: Zhu Zhi-Wen , Li Xin-Miao , Xu Jia
Published: July 2015

A kind of high-aspect-ratio shape memory alloy (SMA) composite wing is proposed to reduce the wing’s fluttering. The nonlinear dynamic characteristics and optimal control of the SMA composite wings subjected to in-plane stochastic excitation are investigated where the great bending under the flight loads is considered. The stochastic stability of the system is analyzed, and the system’s response is obtained. The conditions of stochastic Hopf bifurcation are determined, and the probability density of the first-passage time is obtained. Finally, the optimal control strategy is proposed. Numerical simulation shows that the stability of the system varies with bifurcation parameters, and stochastic Hopf bifurcation appears in the process; the reliability of the system is improved through optimal control, and the first-passage time is delayed. Finally, the effects of the control strategy are proved by experiments. The results of this paper are helpful for engineering applications of SMA.
Clinical and Microbiological Profile of Pathogens in Febrile Neutropenia in Hematological Malignancies: A Single Center Prospective Analysis
Authors: Taj M. , Farzana T. , Shah T. , Maqsood S. , Ahmed S. S. , Shamsi T. S.
Published: July 2015

Background. Febrile neutropenia is the consequence of treatment of hematological disorders. The first-line empirical treatment should cover the prevalent microorganism of the institute. The aim of study was to establish the effectiveness of current practices used at the institution and to review the culture sensitivity pattern of isolated microorganisms.Patients and Methods. Data was recorded and analyzed prospectively for 226 hospitalized patients of febrile neutropenia from January 2011 till December 2013.Results. Out of 226 cases, 173 were males and 53 were females. Clinically documented infections were 104 (46.01%) and microbiologically documented infections were 80 (35.39%), while 42 (18.58%) had pyrexia of undetermined origin. Gram negative infections accounted for 68 (85%) andEscherichia coliwas the commonest isolate. Gram positive microorganisms were isolated in 12 (15%) cases and most common wasStaphylococcus aureus. First-line empirical treatment with piperacillin/tazobactam and amikacin showed response in 184 patients (85.9%) till 72 hours.Conclusion. There is marked decline in infections due to Gram positive microorganisms; however, Gram negative infections are still of great concern and need further surveillance. In this study the antibiogram has shown its sensitivity for empirical antibiotic therapy used; hence, it supports continuation of the same practice.
Crossed Renal Ectopia without Fusion: An Uncommon Cause of Abdominal Mass
Authors: Ratola Ana , Almiro Maria Miguel , Lacerda Vidal Rita , Neves Nuno , Bicho Adelaide , Figueiredo Sofia
Published: July 2015

Crossed renal ectopia is a rare congenital anomaly usually associated with fused kidneys (90%). Most cases are asymptomatic and remain undiagnosed. We report an unusual case of nonfused crossed renal ectopia. The 11-year-old adolescent female patient was admitted with abdominal pain, anorexia, weight loss, and periumbilical mass. Although the initial clinical suspicion was a tumoral lesion, abdominal ultrasound and magnetic resonance examination revealed crossed renal ectopia without fusion. The renal ectopy was incidentally diagnosed, as described in 20 to 30% of cases. In this case, the associated nonspecific symptoms were a coincidence.
Identification of Gene Biomarkers for Distinguishing Small-Cell Lung Cancer from Non-Small-Cell Lung Cancer Using a Network-Based Approach
Authors: Long Fei , Su Jia-Hang , Liang Bin , Su Li-Li , Jiang Shu-Juan
Published: July 2015

Lung cancer consists of two main subtypes: small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC) that are classified according to their physiological phenotypes. In this study, we have developed a network-based approach to identify molecular biomarkers that can distinguish SCLC from NSCLC. By identifying positive and negative coexpression gene pairs in normal lung tissues, SCLC, or NSCLC samples and using functional association information from the STRING network, we first construct a lung cancer-specific gene association network. From the network, we obtain gene modules in which genes are highly functionally associated with each other and are either positively or negatively coexpressed in the three conditions. Then, we identify gene modules that not only are differentially expressed between cancer and normal samples, but also show distinctive expression patterns between SCLC and NSCLC. Finally, we select genes inside those modules with discriminating coexpression patterns between the two lung cancer subtypes and predict them as candidate biomarkers that are of diagnostic use.
Incidence and Predictors of New-Onset Silent Atrial Fibrillation after Coronary Artery Bypass Graft Surgery
Authors: Guenancia Charles , Pujos Charline , Debomy Frederique , Malapert Ghislain , Laurent Gabriel , Bouchot Olivier
Published: July 2015

Aims. We investigated the incidence, risk factors, and prognostic impact of silent atrial fibrillation (AF) after coronary artery bypass graft (CABG) surgery.Methods. This observational study prospectively included 100 patients referred for CABG surgery. Holter ECG monitoring was used to record every arrhythmic event for 7 days. AF was defined as at least one episode >30 s. Episodes recorded on Holter ECG monitoring but not clinically identified were classified as silent AF.Results. Among 34 patients who developed new-onset AF, 13 had silent AF. Compared with patients with maintained sinus rhythm (SR), silent AF patients had a significantly higher logistic EuroSCORE (2.9 (1.5–5.2) versus 2.3 (1.4–3.7),p=0.017) and were more likely to have previous sleep apnea (31% versus 8%,p=0.016) and left atrial diameter >45 mm (36% versus 5%,p=0.002). At one-year follow-up, 30% of silent AF patients had developed symptomatic AF versus 7% in the SR group (p=0.03) and 11% in the clinical AF group (p=0.21).Conclusion. After CABG surgery, silent AF is common and may be associated with a higher incidence of recurrences at one-year follow-up than clinical AF. Improved screening for silent AF may help to reduce thromboembolic events in this high-risk population.
Peripheral Administration of Tumor Necrosis Factor-Alpha Induces Neuroinflammation and Sickness but Not Depressive-Like Behavior in Mice
Authors: Biesmans Steven , Bouwknecht Jan A. , Ver Donck Luc , Langlois Xavier , Acton Paul D. , De Haes Patrick , Davoodi Nima , Meert Theo F. , Hellings Niels , Nuydens Rony
Published: July 2015

Clinical observations indicate that activation of the TNF-αsystem may contribute to the development of inflammation-associated depression. Here, we tested the hypothesis that systemic upregulation of TNF-αinduces neuroinflammation and behavioral changes relevant to depression. We report that a single intraperitoneal injection of TNF-αin mice increased serum and brain levels of the proinflammatory mediators TNF-α, IL-6, and MCP-1, in a dose- and time-dependent manner, but not IL-1β. Protein levels of the anti-inflammatory cytokine IL-10 increased in serum but not in the brain. The transient release of immune molecules was followed by glial cell activation as indicated by increased astrocyte activation in bioluminescent Gfap-luc mice and elevated immunoreactivity against the microglial marker Iba1 in the dentate gyrus of TNF-α-challenged mice. Additionally, TNF-α-injected mice were evaluated in a panel of behavioral tests commonly used to study sickness and depressive-like behavior in rodents. Our behavioral data imply that systemic administration of TNF-αinduces a strong sickness response characterized by reduced locomotor activity, decreased fluid intake, and body weight loss. Depressive-like behavior could not be separated from sickness at any of the time points studied. Together, these results demonstrate that peripheral TNF-αaffects the central nervous system at a neuroimmune and behavioral level.
A Fractional-Order Epidemic Model for Bovine Babesiosis Disease and Tick Populations
Authors: dos Santos José Paulo Carvalho , Cardoso Lislaine Cristina , Monteiro Evandro , Lemes Nelson H. T.
Published: July 2015

This paper shows that the epidemic model, previously proposed under ordinary differential equation theory, can be generalized to fractional order on a consistent framework of biological behavior. The domain set for the model in which all variables are restricted is established. Moreover, the existence and stability of equilibrium points are studied. We present the proof that endemic equilibrium point when reproduction numberR0>1is locally asymptotically stable. This result is achieved using the linearization theorem for fractional differential equations. The global asymptotic stability of disease-free point, whenR0<1, is also proven by comparison theory for fractional differential equations. The numeric simulations for different scenarios are carried out and data obtained are in good agreement with theoretical results, showing important insight about the use of the fractional coupled differential equations set to model babesiosis disease and tick populations.
A Glucosamine-Specific Lectin from Green Dragon No. 8 Beans (Phaseolus vulgaris) Induced Apoptosis on Nasopharyngeal Carcinoma Cells
Authors: Chan Yau Sang , Xia Lixin , Ng Tzi Bun
Published: July 2015

A lectin exhibiting antiproliferative activity on tumor cell lines but devoid of antifungal activity has been purified fromPhaseolus vulgariscv. Green Dragon no. 8 seeds. The lectin was a 60 kDa dimeric protein with two 30 kDa subunits. It was a glucosamine-specific lectin as implied from the inhibitory effect of glucosamine on hemagglutinating activity of the lectin. The steps for isolation of the lectin involved Affi-gel blue gel (affinity gel), Mono Q (anion exchanger), and Superdex 75 column (size exclusion). The lectin was purified 20.8-fold from the crude extract of the beans. The purified lectin showed antiproliferative activity on breast cancer MCF7 cell line and nasopharyngeal cancer HONE1 and CNE2 cell lines, but a low activity on normal skin fibroblast HSF98 cell line. The lectin was shown to induce apoptosis on HONE1 cells, as indicated by increased phosphatidylserine externalization and mitochondrial depolarization. It also blocked HONE1 cell division and kept the cells at the G2/M phase of the cell cycle.
A Compensated Numerical Method for Solving Stochastic Differential Equations with Variable Delays and Random Jump Magnitudes
Authors: Du Ying , Mei Changlin
Published: July 2015

Stochastic differential equations with jumps are of a wide application area especially in mathematical finance. In general, it is hard to obtain their analytical solutions and the construction of some numerical solutions with good performance is therefore an important task in practice. In this study, a compensated split-stepθmethod is proposed to numerically solve the stochastic differential equations with variable delays and random jump magnitudes. It is proved that the numerical solutions converge to the analytical solutions in mean-square with the approximate rate of 1/2. Furthermore, the mean-square stability of the exact solutions and the numerical solutions are investigated via a linear test equation and the results show that the proposed numerical method shares both the mean-square stability and the so-called A-stability.
A Link between the Increase in Electroencephalographic Coherence and Performance Improvement in Operating a Brain-Computer Interface
Authors: Angulo-Sherman Irma Nayeli , Gutiérrez David
Published: July 2015

We study the relationship between electroencephalographic (EEG) coherence and accuracy in operating a brain-computer interface (BCI). In our case, the BCI is controlled through motor imagery. Hence, a number of volunteers were trained using different training paradigms: classical visual feedback, auditory stimulation, and functional electrical stimulation (FES). After each training session, the volunteers’ accuracy in operating the BCI was assessed, and the event-related coherence (ErCoh) was calculated for all possible combinations of pairs of EEG sensors. After at least four training sessions, we searched for significant differences in accuracy and ErCoh using one-way analysis of variance (ANOVA) and multiple comparison tests. Our results show that there exists a high correlation between an increase in ErCoh and performance improvement, and this effect is mainly localized in the centrofrontal and centroparietal brain regions for the case of our motor imagery task. This result has a direct implication with the development of new techniques to evaluate BCI performance and the process of selecting a feedback modality that better enhances the volunteer’s capacity to operate a BCI system.
Study on Individual Traffic Police On-Duty Behavior Analysis Method with Time Series Scheduling
Authors: Peng Huai-jun , Qin Yong , Yang Yan-fang , Zhang Zundong , Chariete Abderrahim
Published: July 2015

In the traditional traffic police scheduling and dispatching, the applications of the position information are restricted. This paper presented a model of the traffic police on-duty behavior analysis based on time series, in order to improve the efficiency of traffic police scheduling and dispatching system. Firstly, it proposes the steps for the behavior analysis of individual traffic police on-duty. Secondly, it elaborates division method of individual traffic police on-duty behavior from background element definition and semantic concept description. Thirdly the paper builds a model concerning individual traffic police on duty behavior by applying state automaton. Finally it describes the implementation methods of key technologies on individual traffic police on duty behavior.
Investigation of the Variability and Implications of Meteorological Dry/Wet Conditions in the Poyang Lake Catchment, China, during the Period 1960–2010
Authors: Ye Xu-chun , Li Yun-liang , Li Xiang-hu , Xu Chong-yu , Zhang Qi
Published: July 2015

This paper presents a quantitative investigation of the variability of meteorological dry/wet conditions of the Poyang Lake catchment during 1960–2010 by using the standardized precipitation-evapotranspiration index (SPEI) based on daily observations of 14 meteorological stations across the catchment. Extending from previous studies, the background of the encounter and overlap of multitimescales of meteorological dry/wet episodes for several severe drought and flood events were discussed. In addition, the possible impacts of temperature changes on dry/wet variability were also examined by the comparison of SPEI and standardized precipitation index (SPI) variations at multitimescales during the study period. Major results show that the occurrence of dry/wet condition has become increasingly frequent in the recent two decades, especially the extreme wet episodes in 1990s and the extreme dry episodes in 2000s. Historically, the encounter and overlap of multitimescales of meteorological dry/wet episodes plays an important role in the occurrence of several severe drought and flood events. The study concluded that the impact of temperature anomaly to the dry/wet variability cannot be neglected.
Pulsed High-Intensity Focused Ultrasound Enhances Delivery of Doxorubicin in a Preclinical Model of Pancreatic Cancer
Authors: Li Tong , Wang Yak-Nam , Khokhlova Tatiana D. , D'Andrea Samantha , Starr Frank , Chen Hong , McCune Jeannine S. , Risler Linda J. , Mashadi-Hossein Afshin , Hingorani Sunil R. , Chang Amy , Hwang Joo Ha
Published: July 2015

Pancreatic cancer is characterized by extensive stromal desmoplasia, which decreases blood perfusion and impedes chemotherapy delivery. Breaking the stromal barrier could both increase perfusion and permeabilize the tumor, enhancing chemotherapy penetration. Mechanical disruption of the stroma can be achieved using ultrasound-induced bubble activity—cavitation. Cavitation is also known to result in microstreaming and could have the added benefit of actively enhancing diffusion into the tumors. Here, we report the ability to enhance chemotherapeutic drug doxorubicin penetration using ultrasound-induced cavitation in a genetically engineered mouse model (KPC mouse) of pancreatic ductal adenocarcinoma. To induce localized inertial cavitation in pancreatic tumors, pulsed high-intensity focused ultrasound (pHIFU) was used either during or before doxorubicin administration to elucidate the mechanisms of enhanced drug delivery (active vs. passive drug diffusion). For both types, the pHIFU exposures that were associated with high cavitation activity resulted in disruption of the highly fibrotic stromal matrix and enhanced the normalized doxorubicin concentration by up to 4.5-fold compared with controls. Furthermore, normalized doxorubicin concentration was associated with the cavitation metrics (P < 0.01), indicating that high and sustained cavitation results in increased chemotherapy penetration. No significant difference between the outcomes of the two types, that is, doxorubicin infusion during or after pHIFU treatment, was observed, suggesting that passive diffusion into previously permeabilized tissue is the major mechanism for the increase in drug concentration. Together, the data indicate that pHIFU treatment of pancreatic tumors when resulting in high and sustained cavitation can efficiently enhance chemotherapy delivery to pancreatic tumors. Cancer Res; 75(18); 3738–46. ©2015 AACR.
Time Trends in Rates of Hodgkin Lymphoma Histologic Subtypes: True Incidence Changes or Evolving Diagnostic Practice?
Authors: Glaser Sally L. , Clarke Christina A. , Keegan Theresa H.M. , Chang Ellen T. , Weisenburger Dennis D.
Published: July 2015

Background: Histologic subtypes of classical Hodgkin lymphoma [cHL; e.g., nodular sclerosis, mixed cellularity, not otherwise specified (NOS)] are epidemiologically and prognostically distinctive. Therefore, unexplained, ongoing incidence rate declines for mixed cellularity and increases for NOS require examination.

Methods: We analyzed detailed histology-specific Hodgkin lymphoma incidence rates in 1992 through 2011 U.S. SEER data (n = 21,372) and reviewed a regional subset of 2007 through 2011 NOS pathology reports for insight into diagnostic practices.

Results: cHL rates were stable until 2007, then decreased for whites [annual percent change (APC) and 95% confidence interval (CI), −3.6% (−5.6% to −1.5%)]. Nodular sclerosis rates declined after 2007 by 5.9% annually, with variation by gender, age, and race/ethnicity. In 1992 through 2011, mixed cellularity rates declined [APC −4.0% (−4.7% to −3.3%)], whereas NOS rates rose [5.3% (4.5%–6.2%)] overall and in most patient groups. The 2007–2011 NOS age-specific rates were more similar to mixed cellularity rates for 1992–1996 than 2007–2011. Trends in combined rates were minimal, supporting increasing misclassification of mixed cellularity, lymphocyte depletion, and specific nodular sclerosis subtypes as NOS. Eighty-eight of 165 reviewed NOS pathology reports addressed classification choice. Twenty (12.1%) justified the classification, 21 (12.7%) described insufficient biopsy material, and coders missed specific subtype information for 27 (16.4%).

Conclusion: Recent nodular sclerosis rate declines largely represent true incidence changes. Long-term rate decreases for mixed cellularity and other less common subtypes, and increases for NOS (comprising ∼30% of cHL cases in 2011), likely reflect changes in diagnostic and/or classification practice.

Impact: Diminishing histologic subtyping undermines future surveillance and epidemiologic study of Hodgkin lymphoma. Guideline-based use of excisional biopsies and more coding quality control are warranted. Cancer Epidemiol Biomarkers Prev; 24(10); 1474–88. ©2015 AACR.
An Integrated Approach for the Identification of HNF4α-Centered Transcriptional Regulatory Networks During Early Liver Regeneration
Authors: Jiao Heng , Zhu Yuping , Lu Shan , Zheng Yongxia , Chen Huan
Published: July 2015

Background/Aims: Hepatocyte nuclear factor-4α (HNF4α), the liver enriched transcription factor (TF), is one of the major regulators of hepatocyte differentiation and proliferation. However, how HNF4α participate in liver regeneration after partial hepatectomy (PH) remains largely unknown. In order to identify the HNF4α-centered regulatory network, we applied an integrated analytic strategy, in which, TF array, mRNA microarray, bioinformatic analysis and ChIP-on-chip assays were integrated. Methods/Results: The TF signatures from MOUSE OATFA (TF-array) platform revealed that the activity of HNF4α was significantly reduced and 17 other TFs showed increased activity at 4 h post PH. Then the ChIP-on-chip analysis on HNF4α were combined with mRNA expression profiling to select the possible HNF4α target genes during early liver regeneration, which were then sub-grouped according to their signaling pathways. More specifically, the HNF4α target genes with the gene ontology (GO) terms of cytokine-cytokine receptor, Jak-STAT, MAPK, toll-like receptor and insulin signaling pathways were further analyzed with an advanced bioinformatics tool named oPOSSUM to identify TF binding sites occupancy and predict the co-regulatory relationship between TFs and targets. Furthermore, we identified that repressed HNF4α during the early phase of liver regeneration may contribute cooperatively to the induction of immediate early genes, such as, c-fos, c-jun and stat3. Conclusion: our data indicate that HNF4α may play an inhibitory role on the induction of specific promitogenic genes and liver regeneration initiation. The integrated approach of mRNA/OATFA/ChIP-DSL/oPOSSUM analysis may help us better characterize the target genes and co-regulatory network of HNF4α during the early stage of liver regeneration.
Interleukin-17 Stimulates STAT3-Mediated Endothelial Cell Activation for Neutrophil Recruitment
Authors: Yuan Shaopeng , Zhang Shimeng , Zhuang Yuanyuan , Zhang He , Bai Jinye , Hou Qi
Published: July 2015

Background/Aims: Interleukin-17 (IL-17) is a major pro-inflammatory cytokine that initiates and maintains inflammation. However, the molecular mechanisms as to how IL-17 influences endothelial cells to promote neutrophil recruitment are not fully understood. Methods: Human endothelial cells (HMECs) were stimulated with IL-17, and investigated for proliferation, migration, and tubule formation activities. Transwell chemotaxis and adhesion assays were performed to assess neutrophil recruitment. Cytokine production was measured by Cytokine Array Chip and ELISA. Western blotting and immunofluorescent analysis were used to detect the phosphorylation and translocation of STAT3. Specific inhibitors, small interfering RNA, and phosphorylation mutants were used to confirm that IL-17 induced STAT3 activation via IL-17RA signaling. Results: Activation of HMECs with IL-17 induced STAT3 phosphorylation and nuclear translocation, which were associated with induction of GRO-α, GM-CSF and IL-8, and neutrophil recruitment. Phosphorylation of STAT3 was identified mainly at the tyrosine in position 705 (Y705), and the Y705F mutants attenuated IL-17-mediated STAT3 activation. Moreover, specific inhibitors, FLLL31, or siRNA silencing of STAT3 attenuated HMECs activation, resulting in inhibition of GRO-α, GM-CSF, IL-8 production, and neutrophil recruitment. Furthermore, phosphorylation of STAT3 was identified as downstream of IL-17RA signaling. Conclusions: IL-17 induced STAT3 activation as a necessary step in endothelial cell activation and neutrophil recruitment.
The Neuroprotective Effect of Liraglutide is Mediated by Glucagon-Like Peptide 1 Receptor-Mediated Activation of cAMP/PKA/CREB Pathway
Authors: Bao Yi , Jiang Lei , Chen Haiyan , Zou Junjie , Liu Zhimin , Shi Yongquan
Published: July 2015

Background/Aims: Glucagon-like peptide-1 (GLP-1)-based drugs are being used to achieve better glucose control in patients with type 2 diabetes, and GLP-1 mimetics such as liraglutide have shown therapeutic potential in preventing diabetes-related microvascular and macrovascular complications as well as comorbidities such as neurodegenerative disorders. In the present study, we investigated the effects of liraglutide on primary rat cortical astrocytes treated with advanced glycation end-products (AGEs). Methods: Gene and protein expression was analyzed by quantitative real time PCR, western blots, and enzyme-linked immunosorbent assay. The caspase-3 activity was assessed using a caspase-3 colorimetric assay kit. The ROS production was measured with CM-H2DCFDA staining. The cell viability of rat astrocytes was determined using MTT assay. Results: Liraglutide ameliorated AGEs-induced oxidative stress, inflammatory cytokine secretion, caspase activation, and cell death in astrocytes, and reversed the AGEs mediated reduction in intracellular cyclic AMP (cAMP) levels, protein kinase A (PKA) activity, and the phosphorylation of the cAMP response element-binding (CREB) protein. The protective effects of liraglutide against AGEs-mediated toxicity were abolished by GLP-1 receptor (GLP-1R) knockdown or pretreatment of cells with the adenylyl cyclase inhibitor SQ22536 or the PKA inhibitor Rp-cAMP. Conclusions: Liraglutide exerts its neuroprotective effects via the GLP-1R-mediated activation of the cAMP/PKA/CREB pathway. The results of the present study support the therapeutic potential of liraglutide for the treatment of neurodegenerative disorders.
Application of Plant Extracts for the Prevention of Dental Erosion: An in situ/in vitro Study
Authors: Weber Marie-Theres , Hannig Matthias , Pötschke Sandra , Höhne Franziska , Hannig Christian
Published: July 2015

Objectives: Antiadherent and antibacterial effects of certain plant extracts have been proven to be beneficial in preventive dentistry. In the present in situ/in vitro crossover study, the impact of plant extracts rich in polyphenols on the erosion-protective properties of the in situ pellicle was evaluated. Methods: Individual splints were prepared for 12 subjects for intraoral exposure of bovine enamel specimens. Following formation of a 1-min pellicle, watery plant extracts (leaves of the wild form of Ribes nigrum, the wild form of Origanum as well as a combination of both) were administered for 10 min in situ. Alternatively, a mouth rinse with fluorides (Elmex Kariesschutz) was performed for 1 min. After further oral exposure for 19/28 min, respectively, slabs were removed and incubated with HCl in vitro over 120 s (pH 2, 2.3, 3). The resulting calcium and phosphate release was quantified photometrically. Slabs with and without a 30-min in situ pellicle served as controls. The modification of pellicle ultrastructure was evaluated by transmission electron microscopy (TEM). Results: Plant extracts modulated the erosion-protective properties of the native in situ pellicle in all test groups in a pH-dependent manner. The combination of R. nigrum leaves and Origanum enhanced the protective properties of the pellicle at all pH values; the administration of this preparation was comparable, yet superior, to the effect of the fluoridated mouth rinse. TEM images indicated that rinsing with R. nigrum leaves/Origanum yielded a distinctly thicker and more electron-dense pellicle. Conclusion: The combination of certain plant extracts offers a novel approach to the complementary prevention of dental erosion.
Threshold Levels of Visual Field and Acuity Loss Related to Significant Decreases in the Quality of Life and Emotional States of Patients with Retinitis Pigmentosa
Authors: Azoulay Line , Chaumet-Riffaud Philippe , Jaron Steven , Roux Camille , Sancho Serge , Berdugo Naomi , Audo Isabelle , Sahel José-Alain , Mohand-Saïd Saddek
Published: July 2015

Introduction: Retinitis pigmentosa (RP) is an inherited retinal disorder, characterized by photoreceptor degeneration inducing progressive vision loss. This study evaluates its impact on quality of life (QOL) and emotional states of patients affected by RP. Methods: A cross-sectional study was conducted on 60 RP patients diagnosed with rod-cone dystrophy and on 20 control subjects. The RP population has been divided into 3 groups according to visual field (VF) and visual acuity (VA) impairments. Concurrently, scores of self-reported QOL (25-item National Eye Institute Visual Functioning Questionnaire) and of the Hospital Anxiety and Depression Scale for anxiety/depression assessments were collected. Results: For the QOL composite score, we noticed consistent differences between all VF and VA affected groups and their control group. We also found significant differences between both the most affected VF group (VF1: ØVF <20°) and VA group (VA1: VA <0.3) compared to other VF and VA groups. For anxiety/depression scores, consistent differences have been found between the control group and VF1 and VA1, respectively. Conclusions: This work determines that, for RP patients, a significant QOL and emotional state deterioration correlates with a residual VF diameter below 20° and a VA lower than 0.3. It introduces, for the first time, thresholds to be used in visual restoration or visual preservation therapies to improve QOL of RP patients.