bluuebunny/crossref_metadata_2025 huggingface dataset:
The Extent and Patterns of Digitalization in Proactive Land Acquisition Strategy (PLAS) Farms in South Africa
Authors: Mazwane Sukoluhle , Makhura Moraka Nakedi , Ginige Athula
Published: October 2024

This study sought to develop an index for agricultural digitalization by applying composite confirmatory analysis (CCA). Another aim was to determine the factors that affect the development of digitalization in PLAS farms. Data on the indicators of the three dimensions of digitalization were collected from 300 Proactive Land Acquisition Strategy (PLAS) farms in South Africa using semi-structured questionnaires. Confirmatory composite analysis (CCA) was employed to reduce the items into three digitalization dimensions and ultimately to a digitalization index. Standardized digitalization index scores were extracted and fitted to a linear regression model to determine the factors affecting digitalization. The results revealed that the model shows practical validity and can be used to measure digitalization as measures of fit (geodesic distance, standardized root mean square residual, and squared Euclidean distance) were all below their respective 95%quantiles of bootstrap discrepancies (HI95 values). Therefore, digitalization is an emergent variable that can be measured using CCA. The average level of digitalization in PLAS farms was 0.02 and varied significantly across provinces. Although farmers have attempted to digitalise their farms, there are still minimal levels of digitalization in PLAS farms. The results further reveal different digitalization patterns. As judged by the estimated weights of various dimensions of digitalization, the use of digital technologies to collect, store, analyse, and disseminate (CSAD) farm-related data contributed more towards the digitalization index. The second most important component of digitalization was automation digitalization. In contrast, value chain digitalization was the least significant contributor. The factors that significantly influence digitalization were age, gender, farm type, network type, and cellular data type. Since PLAS farmers have not embraced much digitalization, it is important to focus on awareness and capacity building. A balanced approach to digitalization would benefit PLAS farms by ensuring that strategies to integrate digital solutions within the value chain are developed. To foster and support the digitalization in PLAS farms, policymakers and stakeholders should tailor their strategies to fit specific socioeconomic factors.
Effect of Crop Geometry and Weed Management Practices on Yield and Yield Attributes of Spring Maize in Banke, Nepal
Authors: Belbase Narayan Prasad , Paudel Shankar , Yadav Rajesh
Published: October 2024

The experiment was conducted in Banke, Nepal, from February 2021 to May 2021 to find suitable spacing and weed management practices for spring maize. The experiment was conducted in a split-plot design in which main plots consisting of two spacings (S1:60 cm × 25 cm and S2:60 cm × 30 cm) and subplots consisting of five weed management practices as, W1: weedy check, W2: weed free, W3: atrazine @ 2 kg a.i. ha-1 as pre-emergence herbicide, W4: atrazine as pre-emergence herbicide followed by single-hand weeding at 30 DAS, and W5: straw mulch. Observations were taken for different parameters, that are, cob length, cob circumference, number of grains per cob, number of ears per hectare, thousand-grain weight, and grain yield. Based on spacing non-significant variations were found for all traits except for the number of ears per hectare. The highest number of ears per hectare (61667 ears ha-1) was found in S1 (60 cm × 25 cm). Based on weed management practices all the traits were significantly different, except the number of ears per hectare and thousand grain weight. The highest value of grain yield (6.15 mt ha-1) was found in straw mulch, and the lowest grain yield (3.72 mt ha-1) was found in the weedy check. A positive correlation was observed between yield and different weed management practices (WEM) (r = 0.5**) and other yield-attributing traits, that are, number of ears per hectare (NE) (r = 0.62**), the number of grains per cob (NK) (r = 0.82**), cob length (CL) (r = 0.47**), cob diameter (CD) (r = 0.68**), and total grain weight (TGW) (r = 0.52**). A negative correlation was observed between yield and spacing (S) (r = -0.41*).
Evaluation of Soumbara (Parkia biglobosa) Quality During Storage Using Mid-Infrared Spectroscopy and Physicochemical Methods
Authors: Sangaré Moriken , Mamady Diawara , Mamadou Lamarana Souaré
Published: October 2024

The fermented seed of néré (Parkia biglobosa), known as Soumbara, is a condiment widely consumed by the population in West Africa and Guinea in particular. The present study aims to use physicochemical, colorimetric methods and mid-infrared (MIR) spectroscopy to evaluate the evolution of the quality of Soumbara during storage (30 days). Thus, 5 kg of Soumbara were taken, subdivided into 7 batches of samples, and analysed every 5 days for 30 days. Physicochemical analyses (water activity, moisture, proteins, and total ash) would show that the storage duration induced a variation during storage; for example, the total ash contents on days 1, 5, 10, 15, 20, 25 and 30 are respectively 3.96 ± 0.25, 3.89 ± 0.43, 3.97 ± 0, 23, 3.99 ± 0.12, 4.54 ± 0.14, 4.57 ± 0.2 and 4.8 ± 0.21%. During the first 15 days of storage, no significant differences (p < 0.05) were observed between samples. The colorimetric measurements showed that the storage duration did not significantly affect the values of a* and b*, except the L* value which decreased significantly (p<0.05) (42.16 ± 0.015 on day 1 to 40.02 ± 0.015 on day 30). Applying statistical methods to mid-infrared spectroscopy allowed a clear distinction between the Soumbara samples during storage for 30 days. The partial least squares regression (PLSR) applied to MIR spectra made it possible to predict water, moisture and protein activity levels, since R2 greater than 0.99 were obtained. The results demonstrated that mid-infrared spectroscopy coupled with chemometric tools could be used as a rapid screening tool to assess and monitor the quality of Soumbara during storage.
Investigation of Rooting Performance of Some Grapevine Rootstocks with Aeroponic Method
Authors: İpek Deniz , Karadağ Hakan
Published: October 2024

In the study, the aeroponic system examined the rooting performances of three grapevine rootstocks (110R, 1103 Paulsen, 5BB) commonly used in Türkiye. IBA was applied to grapevine cuttings to encourage rooting. Grapevine cuttings taken during the winter dormancy period were placed in the aeroponic system after the necessary pretreatments. Significant improvements were observed in root length, root number, and rooting rate during the rooting process of cuttings placed in the aeroponic system. No diseases or pests were observed during the rooting period in the daily checks.
As a result of the study, the highest root length was 17.77 cm in 110R rootstock, while the lowest was 7.1 cm in 1103P rootstock. The highest rooting rate was 76.67% in a 25 ppm IBA application in 1103P rootstock and 73.33% in a 100 ppm IBA application. The lowest rooting was 10% in 110R rootstock at 100 ppm IBA application dose and in the control group. The study's results revealed the positive effects of aeroponic systems in increasing rooting performance. The advantages offered by these systems are essential in terms of sustainability and efficiency in agriculture. Considering global challenges such as climate change and the reduction of agricultural areas, aeroponic systems are expected to find wider applications in the future.
Influence of Grape Heterogeneity on Berry Quality Traits in Table Grapes A Study on the ‘Alphonse Lavallée’ Variety
Authors: Sucu Seda , Topcu Altıncı Neval
Published: October 2024

The study, conducted in 2023 at the vineyard of the Agricultural Research and Application Center of Tokat Gaziosmanpaşa University, focused on the Alphonse Lavallée grape variety, a significant cultivar among table grapes. The research aimed to assess the impact of berry density on various quality parameters. Grape berries were selected across five different density categories (Y0-Y4), including a control group, to evaluate their physical characteristics (including width, length, weight, firmness, and skin color), chemical properties (pH, total titratable acidity, and soluble solids content), and phytochemical attributes (encompassing total phenol content, total antioxidant capacity, and total monomeric anthocyanin levels). The findings revealed that critical quality indicators—namely, soluble solids content, pH, total antioxidant capacity, and total monomeric anthocyanin—were maximized under the Y4 treatment. In contrast, the highest total phenol content was recorded in the Y2 and Y3 treatments. These results underscore the importance of berry density and berry/skin volume as key determinants of grape berry quality. The study contributes valuable insights into the role of these factors in enhancing the overall quality of table grapes, particularly in the context of the Alphonse Lavallée variety.
Characterization of Some Olive Varieties Consumed without Pre-Treatment: Naturally Debittered Olives in Türkiye
Authors: Biçici Yaşar Mert , Elmacı Yeşim
Published: October 2024

In table olive production it is necessary to remove oleuropein by brine or dry salting method because it gives bitterness. However, some olive varieties such as Throuba Thassos-Greece, Djemali-Tunisia can be consumed without any de-bittering process. Some olives in Türkiye are also consumed without any pre-treatment and these are Kilis Yağlık, Butko, Hurma (Erkence cv.), and Nizip Yağlık olives. These naturally de-bittered olives were aimed to be characterized in this research. The lowest moisture content was determined for Kilis Yağlık (6.84%) and the highest moisture content was determined for Butko (50.01%). The oil and protein content of the samples was between, 16.66-68.46% and 0.19-18.13%, respectively. Total phenolic content (mg GAE/100g) of Kilis Yağlık, Butko, Hurma and Nizip Yağlık olive varieties were determined as 458.87, 152.09, 109.73, 234.33, respectively. The lowest antioxidant capacity was determined for Butko and the highest value was determined for Kilis Yağlık. The hardness values of the olives were found between 677.44-3688.06 (g). The L*, a*, b* values of olive samples were found between 26.14-32.05, 2.02-4.78, 2.37- 7.18, respectively. Highest oleic was determined for Hurma, highest linoleic acid was determined for Butko whereas the highest linolenic acid was determined for Nizip Yağlık. Volatile component analysis results of olives showed that 24, 23, 16 and 17 volatile components were detected in Kilis Yağlık, Butko, Hurma and Nizip Yağlık olives, respectively.
Advisor Intervention Training: Diversity and Culture of Inclusion
Authors:   Florida International University, Secules Stephen , Dickerson Darryl , Begum Kali Maimuna , Kumar Nivedita , Bond Bailey
Published: October 2024

This document contains the PowerPoint presentation, the handout, and the script for an interactive theater-based training we created for inclusive graduate advising. The scripts and scenarios were drawn from a graduate student inclusion education research study conducted by FIU student researchers. We created scenarios of inequity on their basis and helped scaffold participants to be able to respond to the inequities. The Bystander Intervention process (adapted from the FIU ADVANCE team) was: Notice, Interpret, Take responsibility, Think strategically, and Act. The decision for how to act in response to the marginalization included actions which are more immediate or more delayed, and more direct or more indirect. This framework and interactive training helps graduate advisors consider the importance of responding to marginalization and inequity and a range of helpful options when doing so.
Engaging as a Force for Equity through our Pedagogy
Authors: Secules Stephen
Published: October 2024

While many aspects of equitable pedagogy can be planned and designed for with go to strategies, we also have to get better at noticing, interpreting, and acting within our own contexts. Learning to think as person who is engaged in work around equity opens up a dimension of our pedagogical work that needs awareness and action. The slides for a presentation on thinking about equity in higher education classrooms, utilizing a 5 step Bystander Intervention process: Notice, Interpret, Take responsibility, Think strategically, and Act (adapted from the ADVANCE grant team at FIU). When deciding how to Act, there are actions which are more immediate or more delayed, and more direct or more indirect. This framework will help instructors think about a range of helpful actions they can take in response to marginalization and inequity.
Power flow analysis and volt/var control strategy of the active distribution network based on data-driven method
Authors: Chen Hui , Zhu Weiping , Liu Liguo , Shi Mingming , Xie Wenqiang , Zhang Chenyu
Published: October 2024

In order to solve the problems of low power flow calculation accuracy and voltage overcrossing of the distribution network caused by large-scale distributed power supply, a data-driven power flow analysis and volt/var optimization control strategy for distribution network are proposed in this paper. Firstly, a data-driven power flow analysis model for the distribution network is proposed, and the nonlinear mapping relationship between the distribution network state and power flow results is described from the data-driven perspective. Secondly, the paper analyzes the influence of photovoltaic power supply on the voltage of the distribution network, and establishes the volt/var optimization model based on photovoltaic power supply. Then, on the basis of data-driven power flow analysis of the distribution network, the distribution network reactive voltage optimization strategy based on data-driven power flow analysis is proposed to ensure the safe and stable operation of distribution network voltage, reduce the operating network loss of distribution network, and realize the distribution network reactive voltage optimization is not affected by factors such as distribution network line parameters. Finally, IEEE 33 node power distribution system is used to verify the effectiveness of the proposed strategy.
Jogos digitais no contexto do letramento em saúde
Authors: Oliveira Fabíola Belkiss Santos de , Diogo Ana Tereza Silva e , Assunção Élida Lúcia Ferreira e , Andrade Maurício Alves de , Oliveira Gláucia Cavalcante , Carvalho Carlos Rogério Pimenta de , Santos Júlia Maria Moreira , Mameluque Felipe , Bicalho Juliano Magno de Valadares , Mameluque Soraya
Published: October 2024

Objetivo: Revisar a literatura sobre a utilização dos jogos digitais (JD) no contexto do Letramento em Saúde (LS). Revisão bibliográfica: O LS é um importante determinante da saúde e um objetivo central para a promoção da saúde pública, podendo ser realizado através de ferramentas, para capacitar os indivíduos na tomada de decisões ativas e informadas relacionadas à saúde, sendo fundamental para reduzir as desigualdades nesta área. Os JD são adequados para oferecer experiências instrucionais ativas e de resposta imediata, sendo considerados ferramentas importantes para o LS. Acredita-se que a aprendizagem baseada em JD é mais envolvente, resultando numa experiência de aprendizagem mais positiva que motiva o aprendizado. A maioria dos artigos revisados concluiu que os JD (com a sua natureza altamente interativa) podem tornar a aprendizagem mais interessante, envolvente e agradável, fazendo com que os jogadores avaliem a experiência de aprendizagem de forma mais positiva e aumentando a sua motivação intrínseca para aprender. Considerações finais: Os JD têm um valor educativo significativo, particularmente na área do LS e do empoderamento, tanto para as crianças, adolescentes e adultos. Apresentam-se como uma tecnologia educativa alternativa para acessar, avaliar, compreender e aplicar as informações em saúde contidas nos jogos.
Anterior ultrasound approach for evaluating posterior shoulder instability in brachial plexus birth palsy: A novel diagnostic technique
Authors: Jerome J. Terrence Jose
Published: October 2024

Brachial plexus birth palsy (BPBP) often leads to shoulder deformities, such as posterior subluxation and internal rotation of the humeral head, which can severely impact a patient’s quality of life. Traditional imaging techniques such as magnetic resonance imaging, while effective, often require sedation or anesthesia, limiting their routine use in pediatric patients. This technical note explores using ultrasound as a non-invasive alternative for evaluating and monitoring shoulder deformities in BPBP patients. We introduce a novel ultrasound method that focuses on the alignment of the coracoid process and the humeral head, revealing significant differences between affected and unaffected shoulders. This technique offers a reliable and accessible tool for early diagnosis and intervention, improving outcomes for patients with BPBP-related shoulder deformities.
Versatility in the Use of Cadaveric Skin Grafts for Wound Management
Authors: Olivera Whyte Lucila , Izquierdo Matías , Gutiérrez Pachón Diana , Rodríguez Juan , Prezzavento Gustavo
Published: October 2024

Background. Cadaveric skin grafts were initially used for the management of acute burn wounds. The biological coverage of the wound improves the quality of the wound bed, which prepares it to receive an autologous skin graft. The benefits of cadaveric skin graft in burn wounds have led to its use in the management of acute and chronic wounds of diverse etiologies. Objective. To evaluate the use of cadaveric skin graft and subsequent autologous split-thickness skin graft (STSG) in the management of wounds of diverse etiologies at a single institution. Materials and Methods. A retrospective analysis was performed of patients with wounds of different etiologies managed with cadaveric skin grafts followed by a second procedure in which autologous STSG was performed from May 2017 through May 2022 in the Plastic and Reconstructive Surgery Department of German Hospital, Buenos Aires, Argentina. Results. A total of 25 patients with wounds of different etiologies were included. The mean affected body surface area (BSA) was 1.87%. The mean engraftment percentage of the cadaveric skin graft was 96.6%. The mean engraftment percentage of the STSG was 90.6%. All patients demonstrated improvement in local edema and inflammation, reduced secretions, and reduced pain after treatment. Two patients (8%) had complications, with 1 case of delayed healing of the donor site and 1 case of hypertrophic scarring. Conclusions. Cadaveric skin graft with subsequent STSG is a simple, safe, and effective alternative for the management of complex wounds of diverse etiologies. This technique is particularly useful in patients with multiple comorbidities who are at risk of recurrence and of developing multiple wounds during their lifetime.
Utility of a Synthetic Hybrid-Scale Fiber Matrix in the Management of Pyoderma Gangrenosum: A Case Study
Authors: Loesel Kaitlyn , Pritchett Blake , Schultz Alexander , Towarnicki Jonathan
Published: October 2024

Background. Pyoderma gangrenosum (PG) is a rare skin condition that is challenging for both patients and physicians alike. It is extremely painful and can become debilitating for many patients. PG has a mortality rate of 16% at 8 years, and patients with PG have a threefold risk of death compared to the general population. Currently, the management of PG is not standardized and is somewhat experimental as these wounds are prone to pathergy. Case Report. A single patient diagnosed with PG of both lower extremities was successfully treated with a protocol that included serial wound debridement with a sterile scrub brush and hydrodebridement followed by application of a synthetic hybrid-scale fiber matrix (SHSFM) dressing. The patient's wounds successfully healed and the resolution was maintained at 2 years, at which time the patient was discharged from the clinic. Conclusion. To the authors' knowledge, this is the first study reporting the use of SHSFM as a primary treatment option for PG.
A Multicenter Retrospective Study Comparing a Polylactic Acid CAMP With Intact Fish Skin Graft or a Collagen Dressing in the Management of Diabetic Foot Ulcers and Venous Leg Ulcers
Authors: Liden Brock , Liu Tiffany , Regulski Matthew , Foster Melissa , DeLeon Ryan , Palazzi Gina , Ramirez-GarciaLuna Jose
Published: October 2024

Background. Venous leg ulcers (VLUs) and diabetic foot ulcers (DFUs) are examples of chronic wounds that pose an ongoing health care challenge. Despite significant progress in managing such wounds, challenges persist that require innovative solutions. Cellular, acellular, and matrix-like products (CAMPs) are advanced therapies designed to facilitate chronic wound healing. Polylactic acid (PLA) is a synthetic material used in alloplastic CAMPs that has shown promise in the management of burns and traumatic injuries. Objective. To retrospectively assess the effect of PLA in promoting chronic wound healing compared with 2 other well-established CAMPs. Materials and Methods. This retrospective chart review, which was conducted in 3 high-volume wound care clinics, aimed to compare the efficacy of 2 advanced wound care products vs a PLA alloplastic CAMP in promoting DFU and VLU closure. The study included 131 wounds treated with a non-CAMP collagen dressing, fish skin grafts (FSGs), or PLA matrices. Inclusion criteria included single Wagner grade 1 or 2 DFUs or Clinical-Etiology-Anatomy-Pathophysiology classification score C6 VLUs, present for at least 12 to 52 weeks, and measuring between 1 cm² and 20 cm². Patients received weekly treatments with 1 of 3 CAMPs, along with standard care. The primary outcome was time to achieve full healing, and the secondary outcome was the proportion of ulcers healed at 12 weeks. Results. The PLA alloplastic CAMP demonstrated superior outcomes, with a 50% and 20% reduction in time to heal DFUs compared with collagen dressings and FSG, respectively (P < .001). For VLUs, PLA exhibited even more impressive results, achieving 95% and 40% reduction compared with collagen and FSG, respectively (P < .001). PLA allografts displayed a 55% higher rate of full healing by 12 weeks compared with collagen dressings, and a 26% higher rate compared with FSG (P < .001). Conclusion. This study highlights the unique attributes of PLA for achieving wound closure. PLA-based alloplastic CAMPs are promising treatments, offering rapid healing, increased closure rates, and multifaceted benefits for wound healing.
Полиморфизм гена NOS1 и риск развития послеродового атонического кровотечения у женщин узбекской этнической группы
Authors: Ашурова У.А. , Нажмутдинова Д.К. , Бобоев К.Т.
Published: October 2024

Введение. Учитывая рост распространенности послеродовых кровотечений, на сегодня проведено немало работ, посвященных патогенезу атонии матки. Тем не менее, изучение и поиск генов-кандидатов, участвующих в процессах развития атонии матки, остаются актуальными. Цель: оценить связь генетического полиморфизма NOS1 (rs41279104) с дисфункцией миометрия и риском развития атонического послеродового кровотечения у женщин узбекской этнической группы. Методы. Нами обследована 101 женщина с послеродовым атоническим кровотечением различной степени тяжести. Всем женщинам были проведены клинико-лабораторные и инструментальные исследования, включающие стандартные методики сбора анамнеза и физикального обследования. Для молекулярно-генетической детекции полиморфизма rs41279104 в гене NOS1 были использованы образцы геномной ДНК. Результаты. При носительстве аллеля А риск развития послеродовой атонии матки у роженицы со сроком гестации 37-42 недели увеличивается в 1,7 раз (χ2 =4,2, OR=1,7, р=0,05). Однако эти выводы основаны на ограниченном объеме данных, и для подтверждения установленной ассоциации необходимо проведение дополнительных исследований с использованием более крупных выборок.

Background. Given the increasing prevalence of postpartum hemorrhage, a lot of work has been done today on the pathogenesis of uterine atony. Nevertheless, the study and search for candidate genes involved in the development of uterine atony remain relevant. Objective: to evaluate association of the genetic polymorphism NOS1 rs41279104 with myometrial dysfunction and the risk of postpartum uterine atony in Uzbek women. Methods. We examined 101 women who developed postpartum atonic bleeding of varying severity. Common laboratory and instrumental examinations were performed on all women. Genomic DNA was used for the detection of the NOS1 rs41279104. Results. Allele A is the risk factor for postpartum uterine atony more than 1.7 times (χ2 = 4.2, OR = 1.7, p = 0.05) in parturient women with a gestational age of 37-42 weeks. Conclusion. The results of our research indicate that the NOS1 rs41279104 has an association with postpartum atonic bleeding. In women with 37-42 weeks of gestation, allelic variant A may be a risk factor for PPH.
Редкие генетические варианты SYNPO2L при раннем инфаркте миокарда
Authors: Мирошникова В.В. , Драчева К.В. , Данилов Л.Г. , Донников М.Ю. , Воробьев А.С. , Морозкина А.В. , Изюмченко А.Д. , Кусакин А.В. , Эйсмонт Ю.А. , Коваленко Л.В. , Урванцева И.А. , Глотов О.С. , Пчелина С.Н.
Published: October 2024

Инфаркт миокарда (ИМ) является одной из самых распространенных причин смерти и инвалидизации взрослого населения. В настоящее время наблюдается рост заболеваемости ИМ в молодом возрасте. Независимым фактором риска ИМ в молодом возрасте является наследственная предрасположенность. В данной работе был проведен поиск редких генетических вариантов, связанных с развитием сердечно-сосудистых заболеваний, путём полноэкзомного секвенирования у 8 пациентов, перенесших ИМ до 45 лет, у которых на предыдущем этапе были исключены основные генетические факторы риска ИМ − моногенные дислипидемии и тромбофилия. У двух пациентов были выявлены патогенные варианты гена SYNPO2L: p.(Arg630Leu) (rs143723429) и не описанный ранее вариант p.(Arg910Gln). Полученные данные дают основание предполагать вовлеченность гена SYNPO2L, кодирующего белок сократительной мышечной функции, в патогенез раннего ИМ.

Myocardial infarction (MI), being the main complication of coronary heart disease (CHD), is one of the most common causes of death and disability in the adult population. Currently, there is an increase in the incidence of MI at a young age. An independent risk factor for MI at a young age is hereditary predisposition. In this work, we searched for rare genetic variants associated with the development of cardiovascular diseases using whole-exome sequencing in patients who had suffered an MI before the age of 45 years, and for whom the main genetic risk factors for MI − monogenic dyslipidemias and thrombophilia – were excluded. In two patients, rare variants of the SYNPO2L gene were identified − p.(Arg630Leu) (rs143723429) and a previously undescribed variant p.(Arg910Gln), leading to amino acid substitutions that can lead to dysfunction of the corresponding protein. The data obtained suggest the involvement of the SYNPO2L gene, encoding a protein of contractile muscle function, in the pathogenesis of early MI.
Митохондриальная миопатия вследствие недостаточности тимидинкиназы 2 (TK2). Результаты селективного скрининга
Authors: Цыганкова П.Г. , Кистол Д.В. , Чаусова П.А. , Захарова Е.Ю.
Published: October 2024

Митохондриальная миопатия, вызванная недостаточностью тимидинкиназы 2 (TK2), представляет собой наследственное моногенное заболевание, входящее в группу синдромов нарушения целостности и репликации митохондриальной ДНК (мтДНК). Эти заболевания, в основном, проявляются тяжелыми мультисистемными детскими формами и характеризуются существенным снижением числа копий мтДНК в пораженных клетках и тканях. В России первые случаи TK2-обусловленной миопатии были выявлены в 2019 г. Важность ранней диагностики заболевания обусловлена появлением новых методов лечения. Цель: проведение селективнного скрининга на TK2-недостаточность среди 150 пациентов с ранее исключенным диагнозом спинальной мышечной атрофии, а также 50 пациентов с предполагаемым диагнозом врожденной миопатии с отрицательным результатом анализа методом панельного секвенирования. Метод исследования: высокопроизводительное секвенирование с использованием панели генов митохондриальных заболеваний. Результаты: выявлен один гетерозиготный носитель патогенного варианта в гене TK2. Дополнительный анализ российских баз данных экзомного секвенирования Ruexac, Ru-seq browser показал расчетную частоту TK2-недостаточности равную 1:563900, доверительные интервалы CI (95%) – 218 894:1 454 451.

Mitochondrial myopathy caused by thymidine kinase 2 (TK2) deficiency is an inherited monogenic disease that is part of a group of mitochondrial DNA (mtDNA) maintenance and replication syndromes. These diseases mainly manifest as severe multisystem childhood forms and are characterized by a significant reduction in mtDNA copies in the affected cells and tissues. In Russia, the first cases of TK2- associated myopathy were identified in 2019. The importance of early diagnosis of the disease is due to the emergence of new treatment methods. Aim of the study: to conduct selective screening for TK2 deficiency among 150 patients with a previously excluded diagnosis of spinal muscular atrophy, as well as 50 patients with a presumptive diagnosis of congenital myopathy with a negative test result using panel sequencing. Method: high-throughput sequencing using a panel of mitochondrial disease genes. Results: One heterozygous carrier of a pathogenic variant in the TK2 gene was identified. Additional analysis of Russian exome sequencing databases Ruexac, Ru-seq browser showed an estimated frequency of TK2 deficiency equal to 1:563900, confidence intervals CI (95%) – 218,894:1,454,451.
Однонуклеотидные замены в генах сигнального пути МАРК (NRAS, KRAS, BRAF) при плазмоклеточных новообразованиях
Authors: Руденкова Т.В. , Климкович Н.Н. , Костюк С.А. , Козич Ж.М.
Published: October 2024

Плазмоклеточные новообразования представляют собой мультифокальную неопластическую пролиферацию плазматических клеток, секретирующих моноклональный белок. При формировании множественной миеломы происходит многоступенчатый процесс трансформации клеток, включающий изменения генетического профиля из-за дополнительных событий, таких как соматические мутации, эпигенетические и хромосомные изменения, что приводит к прогрессированию заболевания от моноклональной гаммапатии неуточненного значения до симптоматической множественной миеломы и, в конечном итоге, у некоторых пациентов к агрессивному экстрамедуллярному заболеванию. Профиль генетических изменений, ассоциированных с прогрессированием плазмоклеточных новообразований, включает однонуклеотидные замены в генах сигнального пути MAPK. Белки семейств RAS и RAF являются компонентами сигнального пути RAS/RAF/MEK/ERK, который переносит внешние сигналы в ядро клетки. Однонуклеотидные замены в генах RAS и RAF приводят к необоснованной активации каскадных реакций сигнального пути MAPK, вызывая изменения клеточного цикла и опосредуя злокачественную трансформацию клеток.

Plasma cell neoplasms are a multifocal neoplastic proliferation of plasma cells that secrete a monoclonal protein. During the formation of multiple myeloma, a multistep process of cell transformation occurs, including changes in the genetic profile due to additional events such as somatic mutations, epigenetic and chromosomal changes, which leads to the progression of the disease from monoclonal gammopathy of unspecified significance to symptomatic multiple myeloma and, ultimately, in some patients to aggressive extramedullary disease. The profile of genetic changes associated with the progression of plasma cell neoplasms includes single nucleotide polymorphisms in the genes of the MAPK signaling pathway. Proteins of the RAS and RAF families are components of the RAS/RAF/MEK/ERK signaling pathway, which transmits external signals into the cell nucleus. Single-nucleotide polymorphisms in the RAS and RAF genes lead to aberrant activation of the MAPK signaling pathway cascade reactions, causing changes in the cell cycle and mediating malignant transformation of cells.
Популяционная структура населения Северной Осетии-Алании
Authors: Ельчинова Г.И. , Тебиева И.С. , Ревазова Ю.А. , Васильева Т.В. , Марахонов А.В. , Кадышев В.В. , Балинова Н.В. , Гинтер Е.К. , Зинченко Р.А.
Published: October 2024

В работе обобщены результаты описания популяционно-генетических характеристик населения Северной Осетии – Алании, полученные в ходе комплексного генетико-эпидемиологического обследования населения Республики. Корреляционный анализ показал хорошее соответствие полученных параметров.

The article summarizes the results of the description of the population and genetic characteristics of the population of North Ossetia - Alania, obtained during a comprehensive genetic and epidemiological survey of the population of the Republic. The correlation analysis showed a good correspondence of the obtained parameters.
Межотраслевые связи как правовая категория в праве социального обеспечения: научно-теоретический аспект
Authors: Корсаненкова Ю.Б.
Published: October 2024

В статье на основе системно-структурного подхода предпринята попытка определить понятие межотраслевых связей права социального обеспечения как новой правовой категории, их юридическое значение для систематизации обширного комплексного законодательства о социальном обеспечении.

The article gives an attempt to define the notion of intersectoral relations of social security law as a new legal category, their legal significance for systematizing the extensive comprehensive legislation on social security.
Библиотеки в Москве: что о них думают молодежь и родители
Authors: Борусяк Л.Ф.
Published: October 2024

В статье анализируется читательская активность московских детей и молодежи и барьеры, ее ограничивающие. Основное внимание уделяется роли библиотек как части читательского пространства. В статье показано отношение молодежи и родителей к московским библиотекам, а также причины невысокой популярности районных библиотек и негативного имиджа библиотеки как социального института. Статья основана на результатах социологических опросов молодежи и родителей о детском чтении, а также фокус-групп, проведенных нами со студентами.

This article studies the intensity of reading by children and youth in Moscow and the barriers limiting it. The author highlights the role of libraries as part of the reading environment. The attitude of the youth and parents of children toward Moscow libraries, the reasons for low popularity of local libraries, and the negative image of libraries as a social institution are given in the article. The article is based on the results of sociological surveys of youth and parents on children's reading, as well as focus groups that we conducted with students.
Метод постановки проблем: практическое решение
Authors: Лях Ю.А.
Published: October 2024

Статья включает описание стратегий обучения, таких как постановка проблемы, поиск решений, выбор типа вопроса. Дается ряд практических советов, чтобы помочь учителю организовать урок и сформировать у учащихся навык систематического обдумывания того, как найти решение различных видов академических и неакадемических проблем.

The article describes learning strategies, such as problem statement, finding solutions, choosing a type of question. The author gives a number of practical tips to help a teacher to organize a lesson and develop students’ skills in systematic thinking about how to find solutions to various types of academic and non-academic problems.
Организационно-правовые модели реализации инклюзивного среднего профессионального образования
Authors: Мануйлова В.В.
Published: October 2024

В статье рассмотрены варианты организации инклюзивного среднего профессионального образования; раскрываются типичные затруднения, которые возникают на практике при реализации инклюзивного образования; дается характеристика общих проблем, характерных для деятельности всех базовых профессиональных образовательных организаций, и способы их устранения.

The article deals with the organizational and legal models of the organization of inclusive secondary vocational education; reveals the typical difficulties that arise in the practice of organization of inclusive education. The common problems and difficulties characteristic of the activities of all basic professional educational organizations and ways to address them are described.
Экофилософия: герменевтический подход
Authors: Шульга Е.Н.
Published: October 2024

Статья посвящена обоснованию герменевтического подхода в познании живого. Определяется место герменевтики в системе философского знания. Герменевтика рассматривается в контексте традиции интерпретации; утверждается идея всеобщности интерпретации знания и ее философско-методологическая роль в интерпретации научных фактов, понимании их смысла.

The article is devoted to the justification of the hermeneutic approach in the knowledge of live being. The place of hermeneutics in the system of philosophical knowledge is determined. Hermeneutics is considered within a context of the tradition of interpretation. An idea of the universality of knowledge interpretation and its philosophical and methodological role in interpreting of scientific facts together with apprehending of their meaning is promoted.
Религиозный смысл искусства
Authors: Сухорукова О.А.
Published: October 2024

В статье рассмотрены исторические особенности формирования принципов религиозного искусства. Ссылаясь на работу Н. Бердяева «Кризис искусства», автор обращает внимание на два новых направления, которые сложились на рубеже XIX–XX вв.: синтетическое и аналитическое. Последнее, по мнению философа, выражает основную проблему искусства — развоплощение, или дематериализацию. Используя данные термины в контексте христианской культуры и догматики, автор сравнивает их эстетические принципы в искусстве дзен-буддизма.
Управление сферой обращения твердых бытовых отходов в России
Authors: Галкин Е.Р.
Published: October 2024

В статье рассмотрены особенности утилизации и использования твердых бытовых отходов в России. Проведен краткий анализ существующего положения дел с отходами в нашей стране.

The article deals with the peculiarities of recycling and the use of municipal solid waste in Russia. A brief analysis of the current situation with waste in our country.
Проблемы, риски и перспективы цифровой экономики в развитии сферы образования
Authors: Байдин Д.И.
Published: October 2024

В статье анализируется проблематика экономического развития страны в связи с интенсивным технологическим прогрессом: конкретизированы особенности цифровой экономики, определена роль влияния цифровых средств обучения на экономический потенциал различных секторов экономики.

The article discusses the problems of the economic development of the country in connection with the intensive technological progress: the specifics of the digital economy are specified, the role of the influence of digital learning tools on the economic potential of various sectors of the economy is defined.
Показатели и критерии качества в здравоохранении
Authors: Швец Ю.Ю.
Published: October 2024

В статье проведен анализ основных тенденций, подходов к созданию и использованию инструментальной базы оценки качества, уровня эффективности деятельности медицинских организаций в РФ и некоторых зарубежных странах. В результате проведенного исследования было выявлено отсутствие единого механизма, позволяющего сопоставлять результативность работы медицинских организаций, которые располагаются в различных субъектах РФ, а также отмечена методологическая разобщенность при выборе показателей и критериев независимой оценки эффективности деятельности медицинских организаций. В недостаточной мере уделяется внимание финансово-экономическим стимулирующим мерам, направленным на повышение медицинской, социальной и экономической эффективности в деятельности медицинских организаций.

The article analyzes the main trends, approaches to the creation and use of the instrumental base for assessing the quality and level of efficiency of the activities of medical organizations in the Russian Federation and some foreign countries. As a result of the study, it revealed the absence of a single mechanism to compare the performance of medical organizations, which are located in different regions of the Russian Federation, and also noted the methodological dissociation in the selection of indicators and criteria for an independent assessment of the effectiveness of medical organizations. Not enough attention is paid to financial and economic incentive measures aimed at improving medical, social and economic efficiency in the activities of medical organizations.
Выявление несоответствия восприятия факторов, влияющих на потребительский опыт
Authors: Швец И.Ю.
Published: October 2024

В статье проанализированы разрывы восприятия факторов, влияющих на потребительский опыт сотрудниками и потребителями в индустрии гостеприимства. Выделены уровни вовлеченности потребителя в процесс взаимодействия с компанией. Проанализированы методы изучения, анализа поведения потребителя. Определены основные факторы, влияющие на удовлетворенность потребителя. Проанализированы основные причины неудовлетворенности клиентов предоставляемыми услугами. Представленная модель качества услуги даст возможность быстро реагировать на изменения внешней среды. Рассмотрены основные критерии прокачественного подхода предоставления услуг в индустрии гостеприимства.

The article analyzes the gaps in the perception of factors influencing consumer experience by employees and consumers in the hospitality industry. The levels of consumer involvement in the process of interaction with the company are highlighted. The methods of study and analysis of consumer behaviour are analyzed. The main factors affecting customer satisfaction are highlighted. The main causes of customer dissatisfaction with facilities services are analyzed. The presented model of service quality will provide an opportunity to quickly respond to changes in the external environment. The main criteria for a quality approach to providing services in the hospitality industry are considered.
ВЛИЯНИЕ ПОЛИМЕРНОГО ГЕЛЯ НА ВЛАЖНОСТЬ ДЕРНОВО-ПОДЗОЛИСТОЙ СУПЕСЧАНОЙ ПОЧВЫ РАЗНОЙ ПЛОТНОСТИ СЛОЖЕНИЯ
Authors: Усков И.Б. , Данилова Т.Н. , Старцев А.С.
Published: October 2024

В связи с происходящими изменениями климата применение гидрогелей приобретает особое значение. Водопоглощающие полимеры позволяют разрабатывать перспективные способы регулирования водного режима почв. В лабораторном эксперименте исследовано влияние полимерного геля Aquasorb на водоудерживающую способность дерново-подзолистой супесчаной почвы и изучены циклы «увлажнение – иссушение» при разной плотности сложения. Влагосодержание дерново-подзолистой почвы с гидрогелем определялось на психрометре, для изучения циклов «увлажнение – иссушение» применялись метод экспресс-сушки (быстрой) и естественное иссушение. Дозы гидрогеля составляли 100 кг га–1, 200 кг га–1, 300 кг га–1и 400 кг га–1. Проведенные исследования показали, что влагоудерживание дерново-подзолистой супесчаной почвы зависит от дозы внесения полимерного геля. Внесение гидрогеля способствовало повышению количества продуктивной влаги (НВ-ВЗ) от 8–9% в контроле до 44,9–46,4% при дозе 400 кг га–1. При изучении циклов «увлажнение – высушивание» в вариантах с экспресс-сушкой образцов дерново-подзолистой супесчаной почвы при уплотнении до 0,9–1 г см–3 и до 1,35 г см–3не выявлено существенного влияния гидрогеля Aquasorb на сохранение почвенной влаги по сравнению с контролем. Влияние гидрогеля на динамику сохранения влаги при естественном иссушении четко прослеживается в диапазоне 20% и ниже по сравнению с контролем только при уплотнении до 0,9–1 г см–3. Наиболее эффективен гидрогель при дозах внесения 300 кг га–1 и 400 кг га–1.
АГРОФИЗИЧЕСКИЕ СВОЙСТВА И ПЛОДОРОДИЕ ПОЧВ СОЛОНЦЕВАТОГО КОМПЛЕКСА В СЕВЕРНОЙ ЛЕСОСТЕПИ ОМСКОЙ ОБЛАСТИ
Authors: Юшкевич Л.В. , Хамова О.Ф. , Ющенко Д.Н. , Бутко А.С.
Published: October 2024

В северной лесостепной почвенно-климатической зоне, с преобладанием почв солонцеватого комплекса, в зернопаровом севообороте с чистым и занятом (донниковом) паром, проведены полевые и лабораторные исследования агрофизического состояния, плодородия солонцовых почв. Выявлено, что в отличие от черноземных почв, иллювиальные солонцовые горизонты (В1, В2) отличаются повышенным набуханием при увлажнении, объемными деформациями и усадкой, уплотнением при высыхании, которые в 1,5–2 раза протекают динамичнее чем в горизонте А1, что затрудняет основную и весеннюю обработку. Возделывание донника в занятом пару повышает пористость, аэрацию, структурное состояние, накопление катиона кальция, изменяется соотношение между жидкой и газообразной фазами в верхнем слое (1:0,82), биологическую активность, накопление N-NO3. Периодическое безотвальное рыхление на глубину 30–35 см, в чистых и занятых парах, сдерживает самоуплотнение и засоление солонцеватых почв восходящими потоками грунтовых вод.
ПЕРСПЕКТИВЫ ИНТРОДУКЦИИ ШТАММА AZOMONAS MACROCYTOGENES OSV-2 В ПОЧВОПОДОБНЫЙ СУБСТРАТ В КАЧЕСТВЕ МИКРОБИОЛОГИЧЕСКОГО УДОБРЕНИЯ В БИОЛОГО-ТЕХНИЧЕСКИХ СИСТЕМАХ ЖИЗНЕОБЕСПЕЧЕНИЯ
Authors: Аболенцева П.А. , Хижняк С.В. , Величко В.В. , Овсянкина С.В. , Чернов В.Е.
Published: October 2024

Цель работы – оценить перспективы интродукции азотфиксирующего штамма Azomonas macrocytogenes OSV-2 в почвоподобный субстрат (ППС), используемый для выращивания растений в биолого-технических системах жизнеобеспечения. Изучали влияние свежеприготовленного ППС и ППС, использованного для выращивания растений, на рост A. macrocytogenes OSV-2 при внесении в среду Эшби в количестве 10 г 100 мл–1 как в нативном виде, так и после стерилизации при 121°C. Использованный нестерильный ППС, использованный стерильный ППС и свежеприготовленный нестерильный ППС статистически значимо снизили средний диаметр микроколоний A. macrocytogenes OSV-2 соответственно на 50,7%, на 34,3% и на 13,8% относительно контроля. В противоположность этому, в присутствии свежего стерильного ППС произошло статистически значимое увеличение среднего размера микроколоний на 29,3% относительно контрольного варианта. Двухфакторный дисперсионный анализ подтвердил, что предварительное выращивание растений на ППС статистически значимо усиливает его бактериостатические свойства в отношении штамма A. macrocytogenesOSV-2, а стерилизация – снижает эти свойства. Полученные результаты означают, что сам по себе ППС обладает рост-стимулирующими свойствами в отношении A. macrocytogenes OSV-2, в то время как микробное сообщество ППС проявляет антагонизм в отношении этого штамма, причём антагонистический эффект усиливается по мере развития этого сообщества. Тем не менее, анализ распределения микроколоний A. macrocytogenes OSV-2 по диаметру в присутствии ППС демонстрирует возможность селекции данного штамма на резистентность к антибиотическим веществам микробного сообщества ППС.