bluuebunny/crossref_metadata_2025 huggingface dataset:
Meta-Analysis of In-Vitro Bonding of Glass-Ionomer Restorative Materials to Primary Teeth
Authors: Peric Tamara , Markovic Evgenija , Markovic Dejan , Petrovic Bojan
Published: July 2021

Restoration of primary teeth is among the main clinical applications of glass-ionomer cements (GIC). The aim of the study was to review and summarize existing evidence of in vitro bond strength of glass-ionomer (GI) restoratives to enamel and dentin of primary teeth. A literature search was performed in PubMed/Medline, Scopus, Web of Science, Cochrane, and Google Scholar databases to identify studies published until April 2021. The search strategy was: (“glass”) and (“ionomer”) and (“primary” or “deciduous”) and (“bond” or “tensile” or “shear”). Two researchers independently retrieved articles that reported on the bond strength of GIC to primary dentin and/or enamel. The meta-analysis was performed to compare the bond strength values of conventional (C) GIC and resin-modified (RM) GIC to different substrates. From 831 potentially eligible articles, 30 were selected for the full-text examination, and 7 were included in the analysis. Studies were rated at high (3), medium (3), and low (1) risk of bias. RM-GIC showed higher bond strength to primary enamel and dentin compared to the C-GIC. Meta-analysis of in vitro studies, evaluating bonding properties of GI restoratives to primary teeth, suggests the superior performance of RM-GIC. However, there is a lack of studies that examine the properties of novel GI formulations.
Microstructure Evolution during Hot Deformation of UNS S32750 Super-Duplex Stainless Steel Alloy
Authors: Cojocaru Elisabeta Mirela , Nocivin Anna , Răducanu Doina , Angelescu Mariana Lucia , Cinca Ion , Balkan Irina Varvara , Șerban Nicolae , Cojocaru Vasile Dănuț
Published: July 2021

The present paper analyzes UNS S32750 Super-Duplex Stainless Steel hot deformation behavior during processing by upsetting. The objective of this paper is to determine the optimum range of deformation temperatures, considering that both austenite and ferrite have different deformation behaviors due to their different morphology, physical, and mechanical properties. Because the capability of plastic deformation accommodation of ferrite is reduced when compared to austenite, side cracks and fissures can form during the hot deformation process. Consequently, it is important to find the optimum conditions of deformation of this type of stainless steel to establish the best processing parameters without deteriorating the material. The experimental program involved the application of hot deformation by the upsetting method on a series of samples between 1000 °C and 1275 °C, with a total degree of deformation of 30%. The resultant samples were examined by SEM-EBSD to establish and analyze the evolution of the phases present in the structure from several points of view: nature, distribution, morphology (size and shape), and their structural homogeneity. The GROD (Grain Reference Orientation Deviation) distribution map was also determined while taking into account the possible precipitation of the secondary austenite phase (γ2-phase) and the analysis of the dynamic recrystallization process according to the applied deformation temperature. The main conclusion was that UNS S32750 SDSS steel can be safely deformed by upsetting between 1050–1275 °C, with an experimented total degree of deformation of 30%.
Silicone Breast Implant Coated with Triamcinolone Inhibited Breast-Implant-Induced Fibrosis in a Porcine Model
Authors: Nam Sun-Young , Ji Han Bi , Shin Byung Ho , Chien Pham Ngoc , Donmez Nilsu , Zhang Xin Rui , Huh Beom Kang , Kim Min Ji , Choy Young Bin , Heo Chan Yeong
Published: July 2021

Cosmetic silicone implants for breast reconstruction often lead to medical complications, such as abnormally excessive fibrosis driven by foreign body granulomatous inflammation. The purpose of this study was to develop a silicone breast implant capable of local and controlled release of a glucocorticoid drug triamcinolone acetonide (TA) for the prevention of silicone-breast-implant-induced fibrosis in a Yorkshire pig model (in vivo). Implants were dip-coated in a TA solution to load 1.85 μg/cm2 of TA in the implant shell, which could release the drug in a sustained manner for over 50 days. Immunohistochemical analysis for 12 weeks showed a decline in tumor necrosis factor-α expression, capsule thickness, and collagen density by 82.2%, 55.2%, and 32.3%, respectively. Furthermore, the counts of fibroblasts, macrophages, and myofibroblasts in the TA-coated implants were drastically reduced by 57.78%, 48.8%, and 64.02%, respectively. The TA-coated implants also lowered the expression of vimentin and α-smooth muscle actin proteins, the major profibrotic fibroblast and myofibroblast markers, respectively. Our findings suggest that TA-coated silicone breast implants can be a promising strategy for safely preventing fibrosis around the implants.
Do Chemical-Based Bonding Techniques Affect the Bond Strength Stability to Cubic Zirconia?
Authors: Comba Allegra , Baldi Andrea , Tempesta Riccardo Michelotto , Carossa Massimo , Perrone Letizia , Saratti Carlo Massimo , Rocca Giovanni Tommaso , Femiano Rossella , Femiano Felice , Scotti Nicola
Published: July 2021

This study evaluated the effectiveness of chemical-based adhesive techniques on promoting immediate and aged bond strength between zirconia and luting cement. A total of 128 discs of zirconia were divided into 4 groups (n = 32) according to the adhesive treatment: tribochemical silica-coating followed by silane (Silane Primer, Kerr) and bonding (Optibond FL, Kerr), Signum Zirconia Bond (Hereaus), Z-Prime Plus (Bisco), and All-Bond Universal (Bisco). Composite cylinders were cemented on the zirconia sample with Duo-Link Universal (Bisco). Eight specimens per group were subjected to 10,000 thermocycles and subsequently bond strength was tested with shear-bond strength test. ANOVA test showed that artificial aging significantly affected the bond strength to zirconia. Bonferroni test highlighted a significant influence of adhesive treatment (Signum) on bond strength after thermocycling. It was concluded that 10-MDP-based bonding systems showed no improvement in initial bond strength compared with tribochemical treatment. All chemical bonding techniques tested in this study were influenced by thermocycling.
Post-Fire Susceptibility to Brittle Fracture of Selected Steel Grades Used in Construction Industry—Assessment Based on the Instrumented Impact Test
Authors: Maslak Mariusz , Pazdanowski Michal , Stankiewicz Marek , Zajdel Paulina
Published: July 2021

The change in the value of the breaking energy is discussed here for selected steel grades used in building structures after subjecting the samples made of them to episodes of heating in the steady-state heating regime and then cooling in simulated fire conditions. These changes were recorded based on the instrumented Charpy impact tests, in relation to the material initial state. The S355J2+N, 1H18N9T steels and also X2CrNiMoN22-5-3 duplex steel were selected for detailed analysis. The fire conditions were modelled experimentally by heating the samples and then keeping them for a specified time at a constant temperature of: 600 °C (first series) and 800 °C (second series), respectively. Two alternative cooling variants were investigated in the experiment: slow cooling of the samples in the furnace, simulating the natural fire progress, without any external extinguishing action and cooling in water mist simulating an extinguishing action by a fire brigade. The temperature of the tested samples was set at the level of −20 °C and alternatively at the level of +20 °C. The conducted analysis is aimed at assessing the risk of sudden, catastrophic fracture of load-bearing structure made of steel degraded as a result of a fire that occurred previously with different development scenarios.
Electrodeposition of the MnO2 on the Ag/Au Core–Shell Nanowire and Its Application to the Flexible Supercapacitor
Authors: Seo Wonbin , Kim Dongwoo , Kim Shihyeong , Lee Habeom
Published: July 2021

Supercapacitors have received considerable attention as energy storage devices owing to their high power density, fast charge/discharge rate, and long cyclic life. Especially with an increasing demand for flexible and wearable devices, research on flexible supercapacitors has surged in recent years. The silver nanowire (Ag NW) network has been used as a flexible electrode owing to its excellent mechanical and electrical properties; however, its use as an electrode for flexible supercapacitors has been limited due to insufficient electrochemical stability. In this study, we proposed a method to resolve this issue. We employed a solution process that enabled the coating of the surface of Ag NW by a thin Au shell of ≈ 5 nm thickness, which significantly improved the electrochemical stability of the Ag NW network electrodes. Furthermore, we confirmed for the first time that MnO2, which is one of the most widely used capacitive materials, can be directly electroplated on the AACS NW network electrode. Finally, we fabricated a high-performance and flexible solid-state supercapacitor using the suggested Ag/Au/MnO2 core–shell NW network electrodes.
Iron-Based Water Treatment Residuals: Phase, Physicochemical Characterization, and Textural Properties
Authors: Likus Magdalena , Komorowska-Kaufman Małgorzata , Pruss Alina , Zych Łukasz , Bajda Tomasz
Published: July 2021

Groundwater treatment residuals (GWTRs) are safe waste materials generated during drinking water treatment. GWTRs are mainly deposited in landfills, but the preferred solution should be reused or utilized for some components. To ensure proper sludge management, it is important to provide quality, chemical composition, and texture characteristics of GWTRs. Therefore, in this study, we aimed to investigate and compare the features of GWTRs collected from four water treatment plants. GWTRs were characterized by X-ray diffraction (XRD); scanning electron microscopy (SEM) with energy dispersion spectroscopy (EDS); Fourier transform infrared spectroscopy (FTIR); thermogravimetric, differential thermogravimetric, and differential thermal analysis (TG, DTG, and DTA, respectively); X-ray fluorescence (XRF); inductively coupled plasma optical emission spectrometry (ICP-OEP); specific surface area (SBET) measurement; and determination of the isoelectric point (pHIEP). According to the results, GWTRs are poor crystalline materials that are predominantly composed of ferrihydrite with minor calcite and quartz admixture. They formed heterogeneously mixed particles with irregular shapes. They were mainly composed of iron oxides (32–55%), silica (4–28%), calcium oxide (4–17%), and manganese oxides (0.3–4.0%). They were found to be mesoporous with a large specific surface area. Due to their composition and texture characteristics, GWTRs demonstrate good adsorption properties toward different compounds such as heavy metals and metalloids.
Experimental Method for Tensile Testing of Unidirectional Carbon Fibre Composites Using Improved Specimen Type and Data Analysis
Authors: Kumar Rajnish , Mikkelsen Lars P. , Lilholt Hans , Madsen Bo
Published: July 2021

This paper presents an experimental method for tensile testing of unidirectional carbon fibre composites. It uses a novel combination of a new specimen geometry, protective layer, and a robust data analysis method. The experiments were designed to test and analyze unprotected (with conventional end-tabs) and protected (with continuous end-tabs) carbon fibre composite specimens with three different specimen geometries (straight-sided, butterfly, and X-butterfly). Initial stiffness and strain to failure were determined from second-order polynomial fitted stress–strain curves. A good agreement between back-calculated and measured stress–strain curves is found, on both composite and fibre level. For unprotected carbon composites, the effect of changing specimen geometry from straight-sided to X-butterfly was an increase in strain to failure from 1.31 to 1.44%. The effect of protection on X-butterfly specimens was an increase in strain to failure from 1.44 to 1.53%. For protected X-butterfly specimens, the combined effect of geometry and protection led to a significant improvement in strain to failure of 17% compared to unprotected straight-sided specimens. The observed increasing trend in the measured strain to failure, by changing specimen geometry and protection, suggests that the actual strain to failure of unidirectional carbon composites is getting closer to be realized.
The Initial Oxidation of HfNiSn Half-Heusler Alloy by Oxygen and Water Vapor
Authors: Appel Oshrat , Cohen Shai , Beeri Ofer , Gelbstein Yaniv , Zalkind Shimon
Published: July 2021

The MNiSn (M = Ti, Zr, Hf) n-type semiconductor half-Heusler alloys are leading candidates for the use as highly efficient waste heat recovery devices at elevated temperatures. For practical applications, it is crucial to consider also the environmental stability of the alloys at working conditions, and therefore it is required to characterize and understand their oxidation behavior. This work is focused on studying the surface composition and the initial oxidation of HfNiSn alloy by oxygen and water vapor at room temperature and at 1000 K by utilizing X-ray photoelectron spectroscopy. During heating in vacuum, Sn segregated to the surface, creating a sub-nanometer overlayer. Exposing the surface to both oxygen and water vapor resulted mainly in Hf oxidation to HfO2 and only minor oxidation of Sn, in accordance with the oxide formation enthalpy of the components. The alloy was more susceptible to oxidation by water vapor compared to oxygen. Long exposure of HfNiSn and ZrNiSn samples to moderate water vapor pressure and temperature, during system bakeout, resulted also in a formation of a thin SnO2 overlayer. Some comparison to the oxidation of TiNiSn and ZrNiSn, previously reported, is given.
Polymer-Based Carriers in Dental Local Healing—Review and Future Challenges
Authors: Kida Dorota , Zakrzewska Aneta , Zborowski Jacek , Szulc Małgorzata , Karolewicz Bożena
Published: July 2021

Polymers in drug formulation technology and the engineering of biomaterials for the treatment of oral diseases constitute a group of excipients that often possess additional properties in addition to their primary function, i.e., biological activity, sensitivity to stimuli, mucoadhesive properties, improved penetration of the active pharmaceutical ingredient (API) across biological barriers, and effects on wound healing or gingival and bone tissue regeneration. Through the use of multifunctional polymers, it has become possible to design carriers and materials tailored to the specific conditions and site of application, to deliver the active substance directly to the affected tissue, including intra-periodontal pocket delivery, and to release the active substance in a timed manner, allowing for the improvement of the form of application and further development of therapeutic strategies. The scope of this review is polymeric drug carriers and materials developed from selected multifunctional groups of natural, semi-synthetic, and synthetic polymers for topical therapeutic applications. Moreover, the characteristics of the topical application and the needs for the properties of carriers for topical administration of an active substance in the treatment of oral diseases are presented to more understand the difficulties associated with the design of optimal active substance carriers and materials for the treatment of lesions located in the oral cavity.
Direct Computation of 3-D Stress Intensity Factors of Straight and Curved Planar Cracks with the P-Version Finite Element Method and Contour Integral Method
Authors: Zhang Jianming , Xu Rui , He Yong , Yang Wensheng
Published: July 2021

This paper presents direct computations of 3-D fracture parameters including stress intensity factors (SIFs) and T-stress for straight and curved planar cracks with the p-version finite element method (P-FEM) and contour integral method (CIM). No excessive singular element or enrichment function is required for the computation. To demonstrate the accuracy and efficiency of the proposed approaches, several benchmark numerical models of 3-D planar straight and curved cracks with single and mixed-mode fractures are considered and analyzed: a through thickness edge straight crack in a homogeneous material, a through thickness inclined straight crack, a penny-shaped crack embedded in a cube and a central ellipse shaped crack in a homogeneous cube. Numerical results are analyzed and compared with analytical solutions and those reported by the extended finite element method (XFEM) and the scaled boundary finite element method (SBFEM) in the available literature. Numerical experiments show the accuracy, robustness and effectiveness of the present method.
Laser Light Trapping Phenomenon in a 3D Radiotherapy Polymer Gel Dosimeter
Authors: Kozicki Marek , Jaszczak Malwina , Dudek Mariusz , Maras Piotr
Published: July 2021

This paper aims to explain the phenomenon of laser light trapping (LLT) in a 3D polymer gel dosimeter. A VIC-T polymer gel dosimeter containing 17% N-vinylpyrrolidone, 8% N,N′-methylenebisacrylamide, 12% tert-butyl alcohol, 5% gelatine, 0.02% hydroquinone and 14 mM tetrakis(hydroxymethyl)phosphonium chloride was used in this study. It was exposed to green laser light with a wavelength of 532 nm. A film was recorded during the exposure. After exposure, Raman spectroscopy was used to study the reactions taking place inside the dosimeter. The obtained results were used to explain what the LLT phenomenon is, what are the consequences for the dosimeter in which such a phenomenon occurs, and what dosimeter components play an important role in the occurrence of LLT. In addition, the conditions under which 3D polymer gel dosimeters can be measured using optical computed tomography at short wavelengths of visible laser light are indicated.
Investigation and Statistical Modeling of the Mechanical Properties of Additively Manufactured Lattices
Authors: Spear Derek G. , Palazotto Anthony N.
Published: July 2021

This paper describes the background, test methodology, and experimental results associated with the testing and analysis of quasi-static compression testing of additively manufactured open-cell lattice structures. The study aims to examine the effect of lattice topology, cell size, cell density, and surface thickness on the mechanical properties of lattice structures. Three lattice designs were chosen, the Diamond, I-WP, and Primitive Triply Periodic Minimal Surfaces (TPMSs). Uniaxial compression tests were conducted for every combination of the three lattice designs, three cell sizes, three cell densities, and three surface thicknesses. In order to perform an efficient experiment and gain the most information possible, a four-factor statistical experimental design was planned and followed throughout testing. A full four-factor statistical model was produced, along with a reduced interactions model, separating the model by the significance of each factor and interaction terms. The impact of each factor was analyzed and interpreted from the resulting data, and then conclusions were made about the effects of the design parameters on the resultant mechanical performance.
Variational Problems with Time Delay and Higher-Order Distributed-Order Fractional Derivatives with Arbitrary Kernels
Authors: Cruz Fátima , Almeida Ricardo , Martins Natália
Published: July 2021

In this work, we study variational problems with time delay and higher-order distributed-order fractional derivatives dealing with a new fractional operator. This fractional derivative combines two known operators: distributed-order derivatives and derivatives with respect to another function. The main results of this paper are necessary and sufficient optimality conditions for different types of variational problems. Since we are dealing with generalized fractional derivatives, from this work, some well-known results can be obtained as particular cases.
Trapezoid and Midpoint Type Inequalities for Preinvex Functions via Quantum Calculus
Authors: Sitho Surang , Ali Muhammad Aamir , Budak Hüseyin , Ntouyas Sotiris K. , Tariboon Jessada
Published: July 2021

In this article, we use quantum integrals to derive Hermite–Hadamard inequalities for preinvex functions and demonstrate their validity with mathematical examples. We use the qϰ2-quantum integral to show midpoint and trapezoidal inequalities for qϰ2-differentiable preinvex functions. Furthermore, we demonstrate with an example that the previously proved Hermite–Hadamard-type inequality for preinvex functions via qϰ1-quantum integral is not valid for preinvex functions, and we present its proper form. We use qϰ1-quantum integrals to show midpoint inequalities for qϰ1-differentiable preinvex functions. It is also demonstrated that by considering the limit q→1− and ηϰ2,ϰ1=−ηϰ1,ϰ2=ϰ2−ϰ1 in the newly derived results, the newly proved findings can be turned into certain known results.
A Novel Reconstruction Method to Increase Spatial Resolution in Electron Probe Microanalysis
Authors: Claus Tamme , Bünger Jonas , Torrilhon Manuel
Published: July 2021

The spatial resolution of electron probe microanalysis (EPMA), a non-destructive method to determine the chemical composition of materials, is currently restricted to a pixel size larger than the volume of interaction between beam electrons and the material, as a result of limitations on the underlying k-ratio model. Using more sophisticated models to predict k-ratios while solving the inverse problem of reconstruction offers a possibility to increase the spatial resolution. Here, a k-ratio model based on the deterministic M1-model in Boltzmann Continuous Slowing-Down approximation (BCSD) will be utilized to present a reconstruction method for EPMA which is implemented as a PDE-constrained optimization problem. Iterative gradient-based optimization techniques are used in combination with the adjoint state method to calculate the gradient in order to solve the optimization problem efficiently. The accuracy of the spatial resolution still depends on the number and quality of the measured data, but in contrast to conventional reconstruction methods, an overlapping of the interaction volumes of different measurements is permissible without ambiguous solutions. The combination of k-ratios measured with various electron beam configurations is necessary for a high resolution. Attempts to reconstruct materials with synthetic data show challenges that occur with small reconstruction pixels, but also indicate the potential to improve the spatial resolution in EPMA using the presented method.
Full-Length Transcriptome of Thalassiosira weissflogii as a Reference Resource and Mining of Chitin-Related Genes
Authors: Cheng Haomiao , Bowler Chris , Xing Xiaohui , Bulone Vincent , Shao Zhanru , Duan Delin
Published: July 2021

β-Chitin produced by diatoms is expected to have significant economic and ecological value due to its structure, which consists of parallel chains of chitin, its properties and the high abundance of diatoms. Nevertheless, few studies have functionally characterised chitin-related genes in diatoms owing to the lack of omics-based information. In this study, we first compared the chitin content of three representative Thalassiosira species. Cell wall glycosidic linkage analysis and chitin/chitosan staining assays showed that Thalassiosira weissflogii was an appropriate candidate chitin producer. A full-length (FL) transcriptome of T. weissflogii was obtained via PacBio sequencing. In total, the FL transcriptome comprised 23,362 annotated unigenes, 710 long non-coding RNAs (lncRNAs), 363 transcription factors (TFs), 3113 alternative splicing (AS) events and 3295 simple sequence repeats (SSRs). More specifically, 234 genes related to chitin metabolism were identified and the complete biosynthetic pathways of chitin and chitosan were explored. The information presented here will facilitate T. weissflogii molecular research and the exploitation of β-chitin-derived high-value enzymes and products.
Monitoring the Emergence of Algal Toxins in Shellfish: First Report on Detection of Brevetoxins in French Mediterranean Mussels
Authors: Amzil Zouher , Derrien Amélie , Terre Terrillon Aouregan , Duval Audrey , Connes Coralie , Marco-Miralles Françoise , Nézan Elisabeth , Mertens Kenneth
Published: July 2021

In France, four groups of lipophilic toxins are currently regulated: okadaic acid/dinophysistoxins, pectenotoxins, yessotoxins and azaspiracids. However, many other families of toxins exist, which can be emerging toxins. Emerging toxins include both toxins recently detected in a specific area of France but not regulated yet (e.g., cyclic imines, ovatoxins) or toxins only detected outside of France (e.g., brevetoxins). To anticipate the introduction to France of these emerging toxins, a monitoring program called EMERGTOX was set up along the French coasts in 2018. The single-laboratory validation of this approach was performed according to the NF V03-110 guidelines by building an accuracy profile. Our specific, reliable and sensitive approach allowed us to detect brevetoxins (BTX-2 and/or BTX-3) in addition to the lipophilic toxins already regulated in France. Brevetoxins were detected for the first time in French Mediterranean mussels (Diana Lagoon, Corsica) in autumn 2018, and regularly every year since during the same seasons (autumn, winter). The maximum content found was 345 µg (BTX-2 + BTX-3)/kg in mussel digestive glands in November 2020. None were detected in oysters sampled at the same site. In addition, a retroactive analysis of preserved mussels demonstrated the presence of BTX-3 in mussels from the same site sampled in November 2015. The detection of BTX could be related to the presence in situ at the same period of four Karenia species and two raphidophytes, which all could be potential producers of these toxins. Further investigations are necessary to understand the origin of these toxins.
Receptor-Interacting Serine/Threonine-Protein Kinase-2 as a Potential Prognostic Factor in Colorectal Cancer
Authors: Jaafar Rola F. , Ibrahim Zeid , Ataya Karim , Hassanieh Joelle , Ard Natasha , Faraj Walid
Published: July 2021

Background and objectives: Receptor-interacting serine/threonine-protein kinase-2 (RIPK2) is an important mediator in different pathways in the immune and inflammatory response system. RIPK2 was also shown to play different roles in different cancer types; however, in colorectal cancer (CRC), its role is not well established. This study aims at identifying the role of RIPK2 in CRC progression and survival. Materials and methods: Data of patients and mRNA protein expression level of genes associated with CRC (RIPK2, tumor necrosis factor (TNF), TRAF1, TRAF7, KLF6, interlukin-6 (Il6), interlukin-8 (Il8), vascular-endothelial growth factor A (VEGFA), MKI67, TP53, nuclear factor-kappa B (NFKB), NFKB2, BCL2, XIAP, and RELA) were downloaded from the PrognoScan online public database. Patients were divided between low and high RIPK2 expression and different CRC characteristics were studied between the two groups. Survival curves were evaluated using a Kaplan–Meier estimator. The Pearson correlation was used to study the correlation between RIPK2 and the other factors. Statistical analysis was carried out using SPSS version 25.0. The Human Protein Atlas was also used for the relationship between RIPK2 expression in CRC tissues and survival. Differences were considered statistically significant at p < 0.05. Results: A total of 520 patients were downloaded from the PrognoScan database, and RIPK2 was found to correlate with MKI67, TRAF1, KLF6, TNF, Il6, Il8, VEGFA, NFKB2, BCL2, and RELA. High expression of RIPK2 was associated with high expression of VEGFA (p < 0.01) and increased mortality (p < 0.01). Conclusions: In this study, RIPK2 is shown to be a potential prognostic factor in CRC; however, more studies are needed to assess and verify its potential role as a prognostic marker and in targeted therapy.
Association of Thyroid Function with Suicidal Behavior: A Systematic Review and Meta-Analysis
Authors: Toloza Freddy J. K. , Mao Yuanjie , Menon Lakshmi , George Gemy , Borikar Madhura , Thumma Soumya , Motahari Hooman , Erwin Patricia , Owen Richard , Maraka Spyridoula
Published: July 2021

Thyroid disease is a very common condition that influences the entire human body, including cognitive function and mental health. As a result, thyroid disease has been associated with multiple neuropsychiatric conditions. However, the relationship between thyroid dysfunction and suicide is still controversial. We conducted a systematic review and meta-analysis to describe the association of thyroid function with suicidal behavior in adults. We searched four data bases (MEDLINE, EMBASE, PsycINFO, and Scopus) from their inception to 20 July 2018. Studies that reported mean values and standard deviation (SD) of thyroid hormone levels [Thyroid-stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), total thyroxine (TT4), and total triiodothyronine (TT3)] in patients with suicidal behavior compared with controls were included in this meta-analysis. The abstracts and papers retrieved with our search strategies were reviewed independently and in duplicate by four reviewers for assessment of inclusion criteria and data extraction, as well as for evaluation of risk of bias. Random-effects models were used in this meta-analysis to establish the mean difference on thyroid function tests between groups. Overall, 2278 articles were identified, and 13 studies met the inclusion criteria. These studies involved 2807 participants, including 826 participants identified with suicidal behavior. We found that patients with suicide behavior had lower levels of FT3 (−0.20 pg/mL; p = 0.02) and TT4 (−0.23 µg/dL; p = 0.045) compared to controls. We found no differences in either TSH, FT4, or TT3 levels among groups. With our search strategy, we did not identify studies with a comparison of overt/subclinical thyroid disease prevalence between patients with and without suicide behavior. The studies included in this meta-analysis had a low-to-moderate risk of bias. In the available literature, the evidence regarding the association of thyroid disorders and suicidal behavior is limited. We found that patients with suicidal behavior have significantly lower mean FT3 and TT4 levels when compared to patients without suicidal behavior. The clinical implications and pathophysiologic mechanisms of these differences remain unknown and further research is needed.
Analysis of Functional Recovery and Subjective Well-Being after Arthroscopic Rotator Cuff Repair
Authors: Adomavičienė Aušra , Daunoravičienė Kristina , Šidlauskaitė Rusnė , Griškevičius Julius , Kubilius Raimondas , Varžaitytė Lina , Raistenskis Juozas
Published: July 2021

Background: Rotator cuff tears are common causes of functional shoulder instability and often lead to arthroscopic rotator cuff repair. A well-programmed rehabilitation leads to successful tendon healing, positive functional recovery and subjective well-being (SWB). Objective: To evaluate the changes in shoulder functioning and SWB pre-, post-outpatient rehabilitation and after one-month follow-up. Materials and Methods: A total of 44 patients were assessed three times: at the beginning (six weeks’ post-surgery), at the end of outpatient rehabilitation (2–3 weeks) and one month after rehabilitation. The outcome measures were the Disabilities of the Arm, Shoulder and Hand score (DASH), active range of motion (ROM), manual muscle testing (MMT), hand dynamometry (HD) and pain level by a Visual Analogue Scale (VAS). SWB was assessed by Rosenberg self-esteem scale (RSES), Positive and Negative Affect Schedule (PANAS) and the Lithuanian Psychological Well-Being Scale (LPWBS). Results are presented as a difference between periods. Results: Affected shoulder motor function (MMT, HD and ROM) significantly improved in three periods (p < 0.05); however, major recovery was observed in the follow-up period. VAS scores meaningfully decreased over all stages and negatively correlated with motor function recovery (p < 0.05). DASH rates exhibited significant retrieval in all phases, especially in follow-up. SWB results demonstrated the larger effects of self-evaluation in follow-up, improved daily functions and psychological wellness, then negative emotions significantly decreased (p < 0.05). Conclusions: The experienced pain and psychosocial factors significantly influence functional recovery of the shoulder during rehabilitation. The improvement in motor function, ability and pain relief during rehabilitation increases level of SWB, psychological wellness and positive emotional affect in long-term context.
Mechanical and Electrical Interaction of Biological Membranes with Nanoparticles and Nanostructured Surfaces
Authors: Raval Jeel , Gongadze Ekaterina , Benčina Metka , Junkar Ita , Rawat Niharika , Mesarec Luka , Kralj-Iglič Veronika , Góźdź Wojciech , Iglič Aleš
Published: July 2021

In this review paper, we theoretically explain the origin of electrostatic interactions between lipid bilayers and charged solid surfaces using a statistical mechanics approach, where the orientational degree of freedom of lipid head groups and the orientational ordering of the water dipoles are considered. Within the modified Langevin Poisson–Boltzmann model of an electric double layer, we derived an analytical expression for the osmotic pressure between the planar zwitterionic lipid bilayer and charged solid planar surface. We also show that the electrostatic interaction between the zwitterionic lipid head groups of the proximal leaflet and the negatively charged solid surface is accompanied with a more perpendicular average orientation of the lipid head-groups. We further highlight the important role of the surfaces’ nanostructured topography in their interactions with biological material. As an example of nanostructured surfaces, we describe the synthesis of TiO2 nanotubular and octahedral surfaces by using the electrochemical anodization method and hydrothermal method, respectively. The physical and chemical properties of these nanostructured surfaces are described in order to elucidate the influence of the surface topography and other physical properties on the behavior of human cells adhered to TiO2 nanostructured surfaces. In the last part of the paper, we theoretically explain the interplay of elastic and adhesive contributions to the adsorption of lipid vesicles on the solid surfaces. We show the numerically predicted shapes of adhered lipid vesicles corresponding to the minimum of the membrane free energy to describe the influence of the vesicle size, bending modulus, and adhesion strength on the adhesion of lipid vesicles on solid charged surfaces.
Ce-Bearing FeSi Alloy Inoculation of Electrically Melted, Low Sulphur Grey Cast Irons for Thin Wall Castings
Authors: Balkan Irina Varvara , Riposan Iulian
Published: July 2021

Electrically melted and over-heated (>1500 °C) grey cast iron at less than 0.04%S, as commonly used, solidifies large amounts of carbides and/or undercooled graphite, especially in thin wall castings; this is necessary to achieve a stronger inoculation. The efficiency of Ce-bearing FeSi alloy is tested for lower ladle addition rates (0.15 and 0.25 wt.%), compared to the base and conventional inoculated iron (Ba,Ca-bearing FeSi alloy). The present work explores chill and associated structures in hypoeutectic grey iron (3.6–3.8%CE, 0.02%S, (%Mn) × (%S) = 0.013–0.016, Alres < 0.002%), in wedge castings W1, W2 and W3 (ASTM A 367, furan resin sand mould), at a lower cooling modulus (1.1–3.5 mm) that is typically used to control the quality of thin wall iron castings. Relatively clear and total chill well correlated with the standard thermal (cooling curve) analysis parameters and structural characteristics in wedge castings, at different wall thickness, displayed as the carbides/graphite ratio and presence of undercooled graphite morphologies. The difference in effects of the two inoculants addition is seen as the ability to decrease the amount of carbides and undercooled graphite, with Ce-bearing FeSi alloy outperforming the conventional inoculant, especially as the wall thickness decreased. It appears that Ce-bearing FeSi alloy could be a solution for low sulphur, electric melt, thin wall iron castings production.
Influence of Pulsed Exposure Strategies on Overhang Structures in Powder Bed Fusion of Ti6Al4V Using Laser Beam
Authors: Grünewald Jonas , Clarkson Pirmin , Salveson Ryan , Fey Georg , Wudy Katrin
Published: July 2021

Manufacturing structures with low overhang angles without support structures is a major challenge in powder bed fusion of metals using laser beam (PBF-LB/M). In the present work, various test specimens and parameter sets with continuous wave (cw) and pulsed exposure are used to investigate whether a reduction of downskin roughness and overhang angle can be achieved in PBF-LB/M of Ti6Al4V. Starting from cw exposure, the limits of overhang angle and surface roughness at the downskin surface are investigated as a reference. Subsequently, the influence of laser power, scanning speed, and hatch distance with fixed pulse duration (τpulse = 25 µs) and repetition rate (υrep = 20 kHz) on surface roughness Ra is investigated. Pulsed exposure strategies enable the manufacturing of flatter overhang angles (≤20° instead of ≥25°). Furthermore, a correlation between the introduced volume energy density and the downskin roughness can be observed for pulsed exposure. As the reduction in volume energy density causes an increase in porosity, the combination of pulsed downskin exposure and commercial cw infill exposure is investigated. The larger the gap in volume energy density between the infill area and downskin area, the more challenging it is combining the two parameter sets. By combining cw infill and pulsed downskin exposure, flatter overhang structures cannot be manufactured, and a reduction in roughness can be achieved.
Metabolomics and Dual RNA-Sequencing on Root Nodules Revealed New Cellular Functions Controlled by Paraburkholderia phymatum NifA
Authors: Bellés-Sancho Paula , Lardi Martina , Liu Yilei , Eberl Leo , Zamboni Nicola , Bailly Aurélien , Pessi Gabriella
Published: July 2021

Paraburkholderia phymatum STM815 is a nitrogen-fixing endosymbiont that nodulate the agriculturally important Phaseolus vulgaris and several other host plants. We previously showed that the nodules induced by a STM815 mutant of the gene encoding the master regulator of nitrogen fixation NifA showed no nitrogenase activity (Fix−) and increased in number compared to P. vulgaris plants infected with the wild-type strain. To further investigate the role of NifA during symbiosis, nodules from P. phymatum wild-type and nifA mutants were collected and analyzed by metabolomics and dual RNA-Sequencing, allowing us to investigate both host and symbiont transcriptome. Using this approach, several metabolites’ changes could be assigned to bacterial or plant responses. While the amount of the C4-dicarboxylic acid succinate and of several amino acids was lower in Fix− nodules, the level of indole-acetamide (IAM) and brassinosteroids increased. Transcriptome analysis identified P. phymatum genes involved in transport of C4-dicarboxylic acids, carbon metabolism, auxin metabolism and stress response to be differentially expressed in absence of NifA. Furthermore, P. vulgaris genes involved in autoregulation of nodulation (AON) are repressed in nodules in absence of NifA potentially explaining the hypernodulation phenotype of the nifA mutant. These results and additional validation experiments suggest that P. phymatum STM815 NifA is not only important to control expression of nitrogenase and related enzymes but is also involved in regulating its own auxin production and stress response. Finally, our data indicate that P. vulgaris does sanction the nifA nodules by depleting the local carbon allocation rather than by mounting a strong systemic immune response to the Fix− rhizobia.
Experimental Protocol to Assess Neuromuscular Plasticity Induced by an Exoskeleton Training Session
Authors: Di Marco Roberto , Rubega Maria , Lennon Olive , Formaggio Emanuela , Sutaj Ngadhnjim , Dazzi Giacomo , Venturin Chiara , Bonini Ilenia , Ortner Rupert , Cerrel Bazo Humberto , Tonin Luca , Tortora Stefano , Masiero Stefano , Del Felice Alessandra ,   on behalf of the PRO GAIT Consortium
Published: July 2021

Exoskeleton gait rehabilitation is an emerging area of research, with potential applications in the elderly and in people with central nervous system lesions, e.g., stroke, traumatic brain/spinal cord injury. However, adaptability of such technologies to the user is still an unmet goal. Despite important technological advances, these robotic systems still lack the fine tuning necessary to adapt to the physiological modification of the user and are not yet capable of a proper human-machine interaction. Interfaces based on physiological signals, e.g., recorded by electroencephalography (EEG) and/or electromyography (EMG), could contribute to solving this technological challenge. This protocol aims to: (1) quantify neuro-muscular plasticity induced by a single training session with a robotic exoskeleton on post-stroke people and on a group of age and sex-matched controls; (2) test the feasibility of predicting lower limb motor trajectory from physiological signals for future use as control signal for the robot. An active exoskeleton that can be set in full mode (i.e., the robot fully replaces and drives the user motion), adaptive mode (i.e., assistance to the user can be tuned according to his/her needs), and free mode (i.e., the robot completely follows the user movements) will be used. Participants will undergo a preparation session, i.e., EMG sensors and EEG cap placement and inertial sensors attachment to measure, respectively, muscular and cortical activity, and motion. They will then be asked to walk in a 15 m corridor: (i) self-paced without the exoskeleton (pre-training session); (ii) wearing the exoskeleton and walking with the three modes of use; (iii) self-paced without the exoskeleton (post-training session). From this dataset, we will: (1) quantitatively estimate short-term neuroplasticity of brain connectivity in chronic stroke survivors after a single session of gait training; (2) compare muscle activation patterns during exoskeleton-gait between stroke survivors and age and sex-matched controls; and (3) perform a feasibility analysis on the use of physiological signals to decode gait intentions.
Surfactants Enhanced Heavy Oil–Solid Separation from Carbonate Asphalt Rocks-Experiment and Molecular Dynamic Simulation
Authors: Hou Jinjian , Du Jinze , Sui Hong , Sun Lingyu
Published: July 2021

In this study, surfactants were used to enhance heavy oil–solid separation, and a detailed mechanism was explored by SARA (saturates, aromatics, resins, asphaltenes) analysis, element analysis, AFM measurement, and molecular dynamic simulation. Surfactants could effectively decrease oil/solid interaction force and then oil–solid separation would be enhanced. The oil–solid interactive force was in relation to surfactants concentration, pH value, asphaltene content, and salinity. The molecular dynamics simulation results show that the dissociation of saturated hydrocarbon, aromatic hydrocarbon, resin, and asphaltene (SARA) on carbonate minerals is gradually weakened for all surfactants. In the process of molecular dynamics simulation of surfactant stripping SARA, firstly, the surfactant molecules adsorb on the surface of SARA molecules. After that, the surfactant peels SARA molecules off the surface of calcite under the influence of molecular thermal motion. In this process, surfactant molecules will not be directly adsorbed on the surface of trace minerals. The results of energy/temperature balance indicated that saturates, aromatics and resins could remain stable when the molecular dynamics simulation time reached 2000 ps with the phenomenon that saturates, aromatics could liberate from minerals totally within 2000 ps. The molecular dynamics simulation of asphaltenes will not liberate from calcite surface within 6000 ps, meanwhile, they could not reach the energy balance/energy balance within 6000 ps. The functional groups of surfactant molecules would have interactions with the SARA functional group, resulting in different dissociation effects of SARA. The results of molecular dynamics simulation are consistent with the experiment results. The separation effect of saturated hydrocarbon, aromatic hydrocarbon, resin, and asphaltene in five kinds of surfactants were different. The molecular dynamic simulation results were in accordance with the SARA analysis.
Free Vibrations of Anisotropic Nano-Objects with Rounded or Sharp Corners
Authors: Saviot Lucien
Published: July 2021

An extension of the Rayleigh–Ritz variational method to objects with superquadric and superellipsoid shapes and cylinders with cross-sections delimited by a superellipse is presented. It enables the quick calculation of the frequencies and displacements for shapes commonly observed in nano-objects. Original smooth shape variations between objects with plane, convex, and concave faces are presented. The validity of frequently used isotropic approximations for experimentally relevant vibrations is discussed. This extension is expected to facilitate the assignment of features observed with vibrational spectroscopies, in particular in the case of single-nanoparticle measurements.
Microbiological Quality of Nuts, Dried and Candied Fruits, Including the Prevalence of Cronobacter spp.
Authors: Berthold-Pluta Anna , Garbowska Monika , Stefańska Ilona , Stasiak-Różańska Lidia , Aleksandrzak-Piekarczyk Tamara , Pluta Antoni
Published: July 2021

Cronobacter genus bacteria are food-borne pathogens. Foods contaminated with Cronobacter spp. may pose a risk to infants or immunocompromised adults. The aim of this study was to determine the microbiological quality of nuts, seeds and dried fruits with special emphasis on the occurrence of Cronobacter spp. Analyses were carried out on 64 samples of commercial nuts (20 samples), dried fruits (24), candied fruits (8), seeds (4), and mixes of seeds, dried fruits and nuts (8). The samples were tested for the total plate count of bacteria (TPC), counts of yeasts and molds, and the occurrence of Cronobacter spp. Cronobacter isolates were identified and differentiated by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragments Length Polymorphism) and RAPD-PCR (Random Amplified Polymorphic DNA by PCR) analysis. TPC, and yeasts and molds were not detected in 0.1 g of 23.4%, 89.1%, and 32.8% of the analyzed samples. In the remaining samples, TPC were in the range of 1.2–5.3 log CFU g−1. The presence/absence of Cronobacter species was detected in 12 (18.8%) samples of: nuts (10 samples), and mixes (2 samples). The 12 strains of Cronobacter spp. included: C. sakazakii (3 strains), C. malonaticus (5), and C. turicensis (4). The results of this study contribute to the determination of the presence and species identification of Cronobacter spp. in products of plant origin intended for direct consumption.