bluuebunny/crossref_metadata_2025 huggingface dataset:
Downregulation of Brain Enriched Type 2 MAGEs Is Associated With Immune Infiltration and Poor Prognosis in Glioma
Authors: Arora Mohit , Kumari Sarita , Singh Jay , Chopra Anita , Chauhan Shyam S.
Published: December 2020

Melanoma associated antigen (MAGE) is an extensively studied family of tumor-associated genes that share a common MAGE homology domain (MHD). Based upon their expression pattern, MAGE genes have been broadly classified into type 1 MAGEs (T1Ms) and type 2 MAGEs (T2Ms) categories. Interestingly, several T2Ms are highly expressed in the brain and involved in the regulation of neuronal development, differentiation, and survival. Available literature suggests possible tumor suppressor functions of a few T2Ms, while information available about their expression, regulation, and clinical significance in glioma is scanty. This prompted us to perform a comprehensive analysis of T2M expression in glioma. Gene expression data from glioma datasets: Oncomine, TCGA, and REMBRANDT study, were used to assess the mRNA expression of T2M genes (MAGED1, MAGED2, MAGED3, MAGED4, MAGED4B, MAGEE1, MAGEE2, MAGEF1, MAGEH1, MAGEL2, NSMCE3, and NDN), and their association with clinical characteristics and composition of the tumor microenvironment. Further, mutation, copy number alteration, and DNA methylation data from TCGA were assessed for determining potential mechanisms of T2Ms expression in glioma. Expression analysis revealed overexpression of MAGED subfamily genes in glioma, while other genes of this family exhibited reduced expression in advanced grades of this malignancy. Further, the expression of T2Ms exhibited varying extent of positive correlations with each other. Amongst downregulated T2Ms, MAGEH1 expression exhibited negative correlations with DNA methylation. Additionally, genes associated with MAGEH1 were enriched in Myc and Hedgehog signaling. Furthermore, T2Ms downregulation was associated with immune infiltration in glioma tissues and poor overall survival of glioma patients. In multivariate Cox regression analysis, MAGEH1 emerged as an independent prognosticator in lower grade glioma. Conclusively, these results suggest that expression of T2Ms is associated with important clinical and molecular features in glioma. Mechanistic studies may further provide novel insights into their role in glioma progression.
Three Immune-Associated Subtypes of Diffuse Glioma Differ in Immune Infiltration, Immune Checkpoint Molecules, and Prognosis
Authors: Zhou Quanwei , Yan Xuejun , Liu Weidong , Yin Wen , Xu Hongjuan , Cheng Damei , Jiang Xingjun , Ren Caiping
Published: December 2020

Diffuse glioma is one of the most prevalent malignancies of the brain, with high heterogeneity of tumor-infiltrating immune cells. However, immune-associated subtypes of diffuse glioma have not been determined, nor has the effect of different immune-associated subtypes on disease prognosis and immune infiltration of diffuse glioma patients. We retrieved the expression profiles of immune-related genes from The Cancer Genome Atlas (TCGA) (n = 672) and GSE16011 (n = 268) cohorts and used them to identify subtypes of diffuse glioma via Consensus Cluster Plus analysis. We used the limma, clusterProfiler, ESTIMATE, and survival packages of R for differential analysis, functional enrichment, immune and stromal score evaluation respectively in three subtypes, and performed log-rank tests in immune subtypes of diffuse glioma. The immune-associated features of diffuse glioma in the two cohorts were characterized via bioinformatic analyses of the mRNA expression data of immune-related genes. Three subtypes (C1–3) of diffuse glioma were identified from TCGA data, and were verified using the GSE16011 cohort. We then evaluated their immune characteristics and clinical features. Our mRNA profiling analyses indicated that the different subtypes of diffuse glioma presented differential expression profile of specific genes and signal pathways in the TCGA cohort. Patients with subtype C1, who were mostly diagnosed with grade IV glioma, had poorer outcomes than patients with subtype C2 or C3. Subtype C1 was characterized by a higher degree of immune cell infiltration as estimated by GSVA, and more frequent wildtype IDH1. By contrast, subtype C3 included more grade II and IDH1-mutated glioma, and was associated with more infiltration of CD4+T cells. Most subtype C2 had the features between subtypes C1 and C3. Meanwhile, immune checkpoints and their ligand molecules, including PD1/(PD-L1/PDL2), CTLA4/(CD80/CD86), and B7H3/TLT2, were significantly upregulated in subtype C1 and downregulated in subtype C3. In addition, patients with subtype C1 exhibited more frequent gene mutations. Univariate and multivariate Cox regression analyses revealed that diffuse glioma subtype was an effective, independent, and better prognostic factor. Therefore, we established a novel immune-related classification of diffuse glioma, which provides potential immunotherapy targets for diffuse glioma.
Role of GPX4-Mediated Ferroptosis in the Sensitivity of Triple Negative Breast Cancer Cells to Gefitinib
Authors: Song Xiang , Wang Xinzhao , Liu Zhaoyun , Yu Zhiyong
Published: December 2020

Gefitinib resistance in triple negative breast cancer (TNBC) is a growing important concern. Glutathione peroxidase 4 (GPX4) is a main regulator of ferroptosis, which is pivotal for TNBC cell growth. We investigated GPX4-mediated ferroptosis in gefitinib sensitivity in TNBC. Gefitinib resistant TNBC cells MDA-MB-231/Gef and HS578T/Gef were constructed and treated with lentivirus sh-GPX4 and ferroptosis inhibitor ferrostatin-1. GPX4 expression, cell viability and apoptosis were detected. Malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) levels were evaluated. The levels of ferroptosis-related proteins were detected. Subcutaneous tumor model was established in nude mice, and gefitinib was intraperitoneally injected to evaluate tumor growth, apoptosis, and Ki-67 expression. GPX4 was increased in gefitinib-resistant cells. After silencing GPX4, the inhibition rate of cell viability was increased, the limitation of colony formation ability was reduced, apoptosis rate was increased, and the sensitivity of cells to gefitinib was improved. After silencing GPX4, MDA and ROS production were increased, while GSH was decreased. Silencing GPX4 promoted ferroptosis. Inhibition of GPX4 promoted gefitinib sensitivity by promoting cell ferroptosis. In vivo experiments also revealed that inhibition of GPX4 enhanced the anticancer effect of gefitinib through promoting ferroptosis. Overall, inhibition of GPX4 stimulated ferroptosis and enhanced TNBC cell sensitivity to gefitinib.
The Role of HLA-G in Tumor Escape: Manipulating the Phenotype and Function of Immune Cells
Authors: Liu Lu , Wang Lijun , Zhao Lihong , He Chen , Wang Ganlu
Published: December 2020

Human leukocyte antigen-G (HLA-G) is a non-classical major histocompatibility complex class I (MHC I) molecule, and under physiological conditions, its expression is strictly restricted to the maternal–fetal interface and immune-privileged organs where HLA-G is expected to contribute to establishment and maintenance of immune tolerance. However, the expression of HLA-G has been found in various types of tumors, and the level of its expression frequently correlates with high-grade histology and poor prognosis, raising the possibility that it may play a negative role in tumor immunity. ILT2 and ILT4, present on a broad of immune cells, have been identified as the main receptors engaging HLA-G, and their interactions have been found to allow the conversion of effectors like NK cells and T cells to anergic or unresponsive state, activated DCs to tolerogenic state, and to drive the differentiation of T cells toward suppressive phenotype. Therefore, tumors can employ HLA-G to modulate the phenotype and function of immune cells, allowing them to escape immune attack. In this review, we discuss the mechanism underlying HLA-G expression and function, its role played in each step of the tumor-immunity cycle, as well as the potential to target it for therapeutic benefit.
Perinatal Mortality and Associated Factors Among Antenatal Care Attended Pregnant Mothers at Public Hospitals in Gamo Zone, Southern Ethiopia
Authors: Dessu Samuel , Dawit Zinabu
Published: December 2020

Introduction: Perinatal mortality is the death of a baby between 28 weeks of gestation onwards and before the first 7 days of life. According to WHO, Ethiopia is one of the most commonly noticed country in the world in considering perinatal mortality rate. The overall perinatal mortality rate in Ethiopia was around 66–124 per 1,000 births.

Objective: To determine the magnitude of perinatal mortality and associated factors among mothers who attended antenatal care at public hospitals in Gamo Zone, Southern Ethiopia.

Methods: A cross-sectional study was conducted at Arba Minch General Hospital and Chencha District Hospital antenatal care attended by pregnant mothers from the 1st of February to the 28th of March 2019, among the mothers enrolled at ANC clinic from the 1st of January to the 30th of December 2018 using a simple random sampling method for the pre-determined 1,820 records. Both bivariate and multivariable logistic regression analysis was conducted. Variables which had a p-value <0.25 in bivariate analysis were considered as a candidate variable for multivariable analysis and variables which had a P-value <0.05 in multivariable analysis were declared as statically significant.

Results: The prevalence of perinatal mortality was 12.6% (95% CI: 11.80, 13.40) and grand multiparity (AOR: 7.40; 95% CI: 2.77, 20.26), having one antenatal visit (AOR: 4.40; 95% CI: 1.64, 11.91), spontaneous vaginal delivery (AOR: 0.36; 95% CI: 0.16, 0.82), being pre-term (AOR: 6.78; 95% CI: 2.41, 19.09), birth weight <2,500 gram (AOR: 3.10; 95% CI: 1.48, 6.46), maternal ever hemoglobin level <10 gm/dl (AOR: 4.04; 95% CI: 1.91, 8.57), and pre-partum onset of pregnancy induced hypertension (AOR: 4.01; 95% CI: 2.01, 6.08) were statistically significant in the multivariable logistic regression model.

Conclusion: The magnitude of perinatal mortality was high as compared with the Ethiopian Health and Demographic Survey report 2016 and high parity, low in number of antenatal care visits, low gestational age, low birth weight, low maternal hemoglobin level, and pre-partum onset of pregnancy induced hypertension were independent factors which increase the perinatal mortality while spontaneous vaginal delivery reduces the mortality risk. Therefore; the community should be educated to reduce the number of instance of births. In addition; the health care professionals should emphasize on the care provided for the newborns having low birth weight and use spontaneous vaginal delivery as much as possible.
Oceanapia magna Sponge Presents Dual Effect on the Gastrointestinal Motility of Rodents: In Vitro and In Vivo Assays
Authors: Pereira Joedna Cavalcante , Figueiredo Indyra Alencar Duarte , de Oliveira Filipe Rodolfo Moreira Borges , Ferreira Sarah Rebeca Dantas , Aires Moreno Giulyane Targino , da Silva Tania Maria Sarmento , Pinheiro Ulisses dos Santos , Santos Barbara Viviana de Oliveira , da Silva Bagnólia Araújo , Cavalcante Fabiana de Andrade
Published: December 2020

Oceanapia magna Santos-Neto, Nascimento, Cavalcanti and Pinheiro sponges are distributed across tropical worldwide seas. Some studies of marine products have shown interesting activities in smooth muscle models. Hence, we assessed the effect of the ethanolic extract of Oceanapia magna. (OC-EtOH) on acute toxicity and gastrointestinal motility (in vitro and in vivo) in rodent models. On guinea pig ileum, OC-EtOH induced a concentration dependent contraction on basal tonus, which was not inhibited by atropine, but in the presence of pyrilamine or verapamil, the effect was antagonized. Contrastingly, on KCl- or histamine-induced contractions, OC-EtOH presented a transient contraction followed by a concentration-dependent relaxation. Moreover, OC-EtOH presented a relaxant profile on cumulative curves to CaCl2 and tonic contraction induced by S-(-)-BayK8644, through Cav blockade. The acute toxicity assay showed that OC-EtOH (2,000 mg/kg, p.o.) did not present any sign of toxicity in female mice. Additionally, OC-EtOH presented antidiarrheal effect in mice, increased the intestinal normal transit and reduced the castor oil-induced intestinal transit. Thus, OC-EtOH presented a dual effect on guinea pig ileum promoting contraction through activation of H1 and CaV, and relaxation through CaV blockade, besides the effect on upper gastrointestinal transit in mice, showing a potential medicinal use of this sponge in intestinal diseases such as diarrhea.
Endothelium-Derived Hyperpolarizing Factor and Myoendothelial Coupling: The in vivo Perspective
Authors: Schmidt Kjestine , de Wit Cor
Published: December 2020

The endothelium controls vascular tone adopting blood flow to tissue needs. It releases chemical mediators [e.g., nitric oxide (NO), prostaglandins (PG)] and exerts appreciable dilation through smooth muscle hyperpolarization, thus termed endothelium-dependent hyperpolarization (EDH). Initially, EDH was attributed to release of a factor, but later it was suggested that smooth muscle hyperpolarization might be derived from radial spread of an initial endothelial hyperpolarization through heterocellular channels coupling these vascular cells. The channels are indeed present and formed by connexins that enrich in gap junctions (GJ). In vitro data suggest that myoendothelial coupling underlies EDH-type dilations as evidenced by blocking experiments as well as simultaneous, merely identical membrane potential changes in endothelial and smooth muscle cells (SMCs), which is indicative of coupling through ohmic resistors. However, connexin-deficient animals do not display any attenuation of EDH-type dilations in vivo, and endothelial and SMCs exhibit distinct and barely superimposable membrane potential changes exerted by different means in vivo. Even if studied in the exact same artery EDH-type dilation exhibits distinct features in vitro and in vivo: in isometrically mounted vessels, it is rather weak and depends on myoendothelial coupling through connexin40 (Cx40), whereas in vivo as well as in vitro under isobaric conditions it is powerful and independent of myoendothelial coupling through Cx40. It is concluded that EDH-type dilations are distinct and a significant dependence on myoendothelial coupling in vitro does not reflect the situation under physiologic conditions in vivo. Myoendothelial coupling may act as a backup mechanism that is uncovered in the absence of the powerful EDH-type response and possibly reflects a situation in a pathophysiologic environment.
Tactile Perception Technologies and Their Applications in Minimally Invasive Surgery: A Review
Authors: Huang Chao , Wang Qizhuo , Zhao Mingfu , Chen Chunyan , Pan Sinuo , Yuan Minjie
Published: December 2020

Minimally invasive surgery (MIS) has been the preferred surgery approach owing to its advantages over conventional open surgery. As a major limitation, the lack of tactile perception impairs the ability of surgeons in tissue distinction and maneuvers. Many studies have been reported on industrial robots to perceive various tactile information. However, only force data are widely used to restore part of the surgeon’s sense of touch in MIS. In recent years, inspired by image classification technologies in computer vision, tactile data are represented as images, where a tactile element is treated as an image pixel. Processing raw data or features extracted from tactile images with artificial intelligence (AI) methods, including clustering, support vector machine (SVM), and deep learning, has been proven as effective methods in industrial robotic tactile perception tasks. This holds great promise for utilizing more tactile information in MIS. This review aims to provide potential tactile perception methods for MIS by reviewing literatures on tactile sensing in MIS and literatures on industrial robotic tactile perception technologies, especially AI methods on tactile images.
Compound C Protects Against Cisplatin-Induced Nephrotoxicity Through Pleiotropic Effects
Authors: Li Fanghua , Sun Anbang , Cheng Genyang , Liu Dong , Xiao Jing , Zhao Zhanzheng , Dong Zheng
Published: December 2020

AICAR (Acadesine/AICA riboside) as an activator of AMPK, can protect renal tubular cells from cisplatin induced apoptosis. But in our experiment, the dorsomorphin (compound C, an inhibitor of AMPK) also significantly reduced cisplatin induced renal tubular cells apoptosis. Accordingly, we tested whether compound C can protect cisplatin-induced nephrotoxicity and the specific mechanism. Here, we treated Boston University mouse proximal tubular cells (BUMPT-306) with cisplatin and/or different dosages of AICAR (Acadesine/AICA riboside) or compound C to confirm the effect of AICAR and compound C in vitro. The AMPK-siRNA treated cells to evaluate whether the protective effect of compound C was through inhibiting AMPK. Male C57BL/6 mice were used to verify the effect of compound C in vivo. Both compound C and AICAR can reduce renal tubular cells apoptosis in dose-dependent manners, and compound C decreased serum creatinine and renal tubular injury induced by cisplatin. Mechanistically, compound C inhibited P53, CHOP and p-IREα during cisplatin treatment. Our results demonstrated that compound C inhibited AMPK, but the renal protective effects of compound C were not through AMPK. Instead, compound C protected cisplatin nephrotoxicity by inhibiting P53 and endoplasmic reticulum (ER) stress. Therefore, compound C may protect against cisplatin-induced nephrotoxicity through pleiotropic effects.
Origins and Stepwise Expansion of R2R3-MYB Transcription Factors for the Terrestrial Adaptation of Plants
Authors: Chang Xiaojun , Xie Shupeng , Wei Lanlan , Lu Zhaolian , Chen Zhong-Hua , Chen Fei , Lai Zhongxiong , Lin Zhenguo , Zhang Liangsheng
Published: December 2020

The R2R3-MYB transcription factors play critical roles in various processes in embryophytes (land plants). Here, we identified genes encoding R2R3-MYB proteins from rhodophytes, glaucophytes, Chromista, chlorophytes, charophytes, and embryophytes. We classified the R2R3-MYB genes into three subgroups (I, II, and III) based on their evolutionary history and gene structure. The subgroup I is the most ancient group that includes members from all plant lineages. The subgroup II was formed before the divergence of charophytes and embryophytes. The subgroup III genes form a monophyletic group and only comprise members from land plants with conserved exon–intron structure. Each subgroup was further divided into multiple clades. The subgroup I can be divided into I-A, I-B, I-C, and I-D. The I-A, I-B, and I-C are the most basal clades that have originated before the divergence of Archaeplastida. The I-D with the II and III subgroups form a monophyletic group, containing only green plants. The II and III subgroups form another monophyletic group with Streptophyta only. Once on land, the subgroup III genes have experienced two rounds of major expansions. The first round occurred before the origin of land plants, and the second round occurred after the divergence of land plants. Due to significant gene expansion, the subgroup III genes have become the predominant group of R2R3-MYBs in land plants. The highly unbalanced pattern of birth and death evolution of R2R3-MYB genes indicates their important roles in the successful adaptation and massive radiation of land plants to occupy a multitude of terrestrial environments.
Intercropping System and N2 Fixing Bacteria Can Increase Land Use Efficiency and Improve the Essential Oil Quantity and Quality of Sweet Basil (Ocimum basilicum L.)
Authors: Kordi Sajad , Salmasi Saeid Zehtab , Kolvanagh Jalil Shafagh , Weisany Weria , Shannon Dennis A.
Published: December 2020

Intercropping fodder plants with medicinal plants, in addition to enhancing productivity, can remarkably reduce the population of weeds, pests and diseases and for naturally meeting of livestock medicinal needs. Two experiments were conducted to evaluate biological yield, essential oil (EO) composition and yield of sweet basil (Ocimum basilicum L.) treated with N2 fixing bacteria in additive intercropping with forage maize during the 2018 and 2019. Treatments were arranged in factorial split-plot-in time in randomized complete block design with three replications. The factors were 100% chemical fertilizer (N), N2 fixing bacteria (Azospirillum brasilense and Azotobacter chroococcum), integration of N2 fixing bacteria + 50% nitrogen chemical fertilizer and control. The cropping pattern factor included of sole cropping basil and the additive intercropping of maize + 25% basil, maize + 50% basil, maize + 75% basil, and maize + 100% basil. The results indicated that the highest essential oil yield (30.8 kg ha−1) and essential oil percentage (0.75%) were obtained in sole cropping with A. brasilense and A. chroococcum + 50% chemical nitrogen fertilizer application in second harvest in 2019. In both cropping systems, the N2 fixing bacteria application significantly increased fresh and dry yield and land equivalent ratio (LER) as compared to control plants. In both years of experiments could remarkably vary depending on type of treatment. In both years, eight constituents including methyl chavicol (17.24–51.28%), Z-citral (neral) (8.33–24.3%), geranial (10.2–31.3%), (E)-caryophyllene (1.05–5.64%), α-trans-bergamotene (0.53–1.7%), α-humulene (0.4–1.69%), germacrene-D (0.2–1.88%), and (Z)-α- bisabolene (1.16–3.86%) were the main constituents of EO. The highest content of methyl chavicol was found through sole cropping of sweet basil with nitrogen chemical fertilizer followed by sole cropping of sweet basil with an integration of A. brasilense and A. chroococcum + 50% nitrogen chemical fertilizer in 2018 and 2019. Intercropping system and N2 fixing bacteria can be effective in reducing chemical fertilizer consumption and environmental pollution and achieving the sustainable agriculture goals.
The Role of Context in Language Development for Children With Autism Spectrum Disorder
Authors: Sánchez Pérez Patricia , Nordahl-Hansen Anders , Kaale Anett
Published: December 2020

Parent and preschool teacher ratings of the 10 noun categories of MacArthur-Bates Communication Development Inventory (CDI) were used to study expressive language in 2–4-year-old children with autism spectrum disorder (ASD) (N = 58) across the home and preschool context. There was no significant difference in the total number of words the children said in the two contexts, but the children said significantly more words in the noun categories “Furniture and rooms” and “People” at home. Only one third of the words the children said were said both at home and in the preschool, while the other two thirds were said only at home or only in preschool. This suggests that what words the children use across contexts differ substantially and that their vocabulary is larger than it seems when measured only in one context. This novel study highlights the importance of assessing the language in children with ASD in multiple contexts in order to better measure their vocabulary and to design appropriate language interventions.
Nostalgia and Heroism: Theoretical Convergence of Memory, Motivation, and Function
Authors: Allison Scott T. , Green Jeffrey D.
Published: December 2020

This article seeks to develop theoretical convergences between the science of nostalgia and the science of heroism. We take four approaches in forging a conceptual relationship between these two phenomena. First, we examine the definitions of nostalgia and heroism from scholars, laypeople, and across cultures, noting how the history of defining the two phenomena has shaped current conceptualizations. Second, we demonstrate how nostalgic experiences consist of reminiscences about our own personal heroism and about cultural role models and heroes. A review of heroism research, moreover, shows also that our recall of our heroes and of heroism is tinged with nostalgia. Third, we make linkages between heroism and nostalgia research focusing on functions, inspiration, sociality, and motivation. Nostalgia researchers have illuminated the functions of nostalgia implicating the self, existential concerns, goal pursuit, and sociality. Our review shows that heroism researchers invoke similar categories of hero functionality. Finally, we propose three areas of future research that can profit from the merging of nostalgia and heroism science, involving the mechanisms by which (a) heroism can fuel nostalgia, (b) nostalgia can promote heroic action, and (c) wisdom results from nostalgic reverie.
Subjective Rank of the Competition as a Factor Differentiating Between the Affective States of Swimmers and Their Sport Performance
Authors: Samełko Aleksandra , Guszkowska Monika , Kuk Anna
Published: December 2020

Purpose

The aim of the study was to establish the differences in affective states of swimmers depending on the subjective rank of the competition and the relationship between affective states and performance in sports competitions of low, medium and high subjectively perceived rank.

Methods

The respondents (n = 31) aged from 15 to 23 years (18.1 ± 2.397) were studied using the psychological questionnaires Perceived Stress Scale (PSS-10), Profile of Mood State (POMS), and Positive and Negative Affect Schedule (PANAS) during sports events. 362 measurements using POMS and 232 measurements using PANAS before the starts were collected. The significance of intergroup differences was determined using the Kruskal-Wallis test. A stepwise regression analysis was used to determine the emotional predictors of sports results.

Results

Subjective rank of sports competition differentiated significantly anger (chi2 = 6.826; p = 0.033), confusion (chi2 = 11.345; p = 0.003), depression (chi2 = 10.2; p = 0.006), fatigue (chi2 = 49.394; p ≤ 0.001), vigour (chi2 = 11.345; p ≤ 0.001), positive emotions (chi2 = 51.233; p ≤ 0.001), and negative emotions (chi2 = 11.552; p = 0.003). Regression analysis showed the influence of mood states and positive emotions on the sports result.

Conclusion

The swimmers’ affective state changed depending on the subjective rank of the competition. Depression and positive emotional state made it possible to predict the result in medium- and high-rank competition.
The Impact of COVID-19-Related Shutdown Measures on the Training Habits and Perceptions of Athletes in the United States: A Brief Research Report
Authors: Jagim Andrew R. , Luedke Joel , Fitzpatrick Austin , Winkelman Greg , Erickson Jacob L. , Askow Andrew T. , Camic Clayton L.
Published: December 2020

The purpose of the current study was to examine the impact of COVID-19 government-enforced shutdown measures on the training habits and perceptions of athletes. A web-based electronic survey was developed and distributed online to athletes. The survey contained questions regarding currently available resources, changes in weekly training habits, and perceptions of training such as intensity, motivation, and enjoyment. A total of 105 (males: n = 31; females: n = 74) athletes completed the survey (mean ± SD age = 19.86 ± 2.13 years). Ninety-nine (94.3%) athletes continued to receive guidance from their primary sport coach or strength training staff. There was a significant (p < 0.001) decrease (mean ± SD) in self-reported participation time for strength training (−1.65 ± 4.32 h. week−1), endurance (−1.47 ± 3.93 h. week−1), and mobility (−1.09 ± 2.24 h. week−1), with the largest reduction coming from participation time in sport-specific activities (−6.44 ± 6.28 h. week−1) pre- to post-shutdown. When asked to rate their current state of emotional well-being using a visual analog scale of 0–100, with 100 being exceptional, the mean score was 51.6 ± 19.6 AU. Athletes experienced notable reductions in training frequency and time spent completing various training related activities. In the future, practitioners should have preparations in place in the event of another lockdown period or future pandemic to avoid or minimize significant disruptions in training. Special considerations may be needed when athletes are allowed to return to sport in the event of significant levels of detraining that may have occurred.
Enhanced Crop Productivity and Sustainability by Using Native Phosphate Solubilizing Rhizobacteria in the Agriculture of Arid Zones
Authors: Maldonado Stefanie , Rodríguez Andrés , Ávila Bastián , Morales Paloma , González Máximo Patricio , Araya Angel Juan Pablo Andres , Olalde Victor , Bravo Jaime , Jana Constanza , Sierra Carlos , Stoll Alexandra
Published: December 2020

The importance of phosphate solubilizing rhizobacteria (PSB) has been well-document as an option for enhancing sustainable agriculture. As a particular group of plant growth promoting rhizobacteria (PGPR), PSB play an important role in the soil phosphorus cycle, increasing the bioavailability for growth and plant development. This study analyses the plant growth promoting effects of 5 strains (BN0009, BN0013, BN0015, BN0024, and BN0035) out of 180 isolated from Jarava frigida (Phil.) F.Rojas (Poaceae), a native grass from the Andean Atacama desert from North of Chile. The five bacterial isolated (BN strains) were identified as non-pathogenic Erwinia sp. and show a high phosphate solubilization capacity for Ca(PO4) ranging from 608.9 to 781.4 mg/L. Strains IAA production varies between 23.5 and 35.9 mg/L, siderophores, phosphatase (alkaline and acid) production was also observed, but none of the five isolated presented antagonism against plant pathogens Botrytis sp. and Sclerotinia sp. All isolates enhanced seed germination in Lactuca sativa and Solanum lycopersicum (excepting BN009). Additionally, all strains stimulated the early root elongation and seedling development in lettuce and tomato. Pot experiments displayed that BN0015, BN0024, and BN0035 significantly promote plant growth regarding root and leaf area, root and leaf weight, as well as leaf number compared with non-treated plants. In a field experiment with lettuce and two fertilization treatments (50 and 100% of the recommended crop fertilization), BN0024 application improved crop productivity compared to respective control. P content in plants with bacterial inoculations increased significantly compared to control in either fertilization treatment, suggesting an improved nutrient uptake. Also, lettuce with 50% fertilization and inoculation with BN0024 equate productivity with the control 100% fertilization. Finally, we discuss these results in the context of applicability to enhance the agroecosystem productivity in arid and semiarid zones.
Bacterial Cross-Contamination in a Veterinary Ophthalmology Setting
Authors: Gentile Dominic , Allbaugh Rachel A. , Adiguzel Mehmet C. , Kenne Danielle E. , Sahin Orhan , Sebbag Lionel
Published: December 2020

The present study describes the prevalence of bacterial cross-contamination in a veterinary ophthalmology setting, a serious issue that can result in healthcare-associated (or nosocomial) infections among patients and staff. Retrospective (n = 5 patients) and prospective (n = 23 patients) studies evaluated bacterial isolates in companion animals presenting with ulcerative keratitis, sampling the patients' cornea and surrounding examination room, including the environment (exam table, countertop, floor) and ophthalmic equipment (slit lamp, transilluminator, direct ophthalmoscope, indirect headset, tonometer). Results of bacterial culture and antibiotic susceptibility testing were recorded, and degree of genetic relatedness was evaluated in six pairs of isolates (cornea + environment or equipment) using pulse-field gel electrophoresis (PFGE). Overall contamination rate of ophthalmic equipment, environment, and examination rooms (equipment + environment) was 42.9% (15/35 samples), 23.7% (9/38 samples) and 32.9% (24/73 samples), respectively. Methicillin-resistant Staphylococcus pseudintermedius (MRSP), a multi-drug resistant (MDR) pathogen with zoonotic potential, was isolated in 8.2% (6/73) of samples. The patient's cornea was likely the source of cross-contamination in 50% (3/6) of MRSP pairs as evaluated by PFGE; notably, two of the three similar bacterial strains did not have an exact match of their antibiotic susceptibility profiles, highlighting the importance of advanced diagnostics such as PFGE to assess cross-contamination in healthcare facilities. Future work could examine the contamination prevalence of specific equipment or the efficacy of cleaning protocols to mitigate cross-contamination in veterinary practice.
Development and Application of a Prioritization Tool for Animal Health Surveillance Activities in Ireland
Authors: Clarke AnneMarie , More Simon J. , Maher James W. , Byrne Andrew W. , Horan Michael , Barrett Damien
Published: December 2020

Decisions around animal health management by stakeholders are often subject to resource limitation, therefore prioritization processes are required to evaluate whether effort is attributed appropriately. The objectives of this study were to develop and apply a surveillance prioritization process for animal health surveillance activities in Ireland. An exploratory sequential mixed research methods design was utilized. A prioritization tool was developed for surveillance activities and implemented over two phases. During the first phase, a survey was conducted which asked stakeholders to prioritize diseases/conditions by importance for Irish agriculture. In the second phase, experts identified the most important surveillance objectives, and allocated resources to the activities that they considered would best meet the surveillance objectives, for each disease/condition. This study developed a process and an accompanying user-friendly practical tool for animal disease surveillance prioritization which could be utilized by other competent authorities/governments. Antimicrobial resistance and bovine tuberculosis were ranked top of the endemic diseases/conditions in the Irish context, while African swine fever and foot and mouth disease were ranked top of the exotic diseases/conditions by the stakeholders. The study showed that for most of the diseases/conditions examined in the prioritization exercise, the respondents indicated a preference for a combination of active and passive surveillance activities. Future extensions of the tool could include prioritization on a per species basis.
Moderate Coconut Oil Supplement Ameliorates Growth Performance and Ruminal Fermentation in Hainan Black Goat Kids
Authors: Shi Liguang , Zhang Yu , Wu Lingli , Xun Wenjuan , Liu Qiang , Cao Ting , Hou Guanyu , Zhou Hanlin
Published: December 2020

The study investigated amelioration effects of coconut oil (CO) on growth performance, nutrient digestibility, ruminal fermentation, and blood metabolites in Hainan Black goat kids. Twenty-four Hainan Black goat kids (10 days of age) were assigned randomly to four treatments for 90 days, including pre-weaning (10–70 d of age) and post-weaning (70-100 d of age) days. The treatment regimens were control (CON), low CO (LCO), medium CO (MCO), and high CO (HCO) with 0, 4, 6, 8 g CO per goat per day, respectively. During the pre-weaning period, the average daily gain (ADG) linearly and quadratically increased (P < 0.05), whereas the average daily feed intake (ADFI) linearly decreased, and the feed conversion ratio (FCR) also decreased linearly and quadratically by increasing CO supplementation (P < 0.05). During the post-weaning period, increasing CO supplementation linearly and quadratically increased the BW at 100 days and ADG (P < 0.05), but quadratically decreased the ADFI and FCR (P < 0.05). The digestibility of ether extract (EE) linearly and quadratically increased with increasing CO supplementation (P < 0.05). Supplementation of CO linearly increased ruminal pH (P < 0.05), but linearly decreased (P < 0.05) ammonia-N, total VFAs, molar proportions of acetate, ruminal microbial enzyme activity of carboxymethyl-cellulase, cellobiase, xylanase, pectinase and α-amylase, and number of total protozoa, the abundance of Ruminococcus albus, Ruminococcus flavefaciens, Fibrobacter succinogenes, Butyrivibrio fibrisolvens, Prevotella ruminicola, and Ruminobacter amylophilus. The estimated methane emission decreased linearly and quadratically with increasing CO addition (P < 0.05). The serum concentration of triglycerides (TG), non-esterified fatty acids (NEFA) and growth hormone (GH) linearly (P < 0.05) increased by raising the CO supplementation. The present results indicate that CO supplementation at 6 g/day per goats is optimum due to improved growth performance and decreased estimated methane emission. Supplementation CO up to 8 g/day depressed growth and feed conversion due to its suppression of growth performance, rumen protozoa, cellulolytic bacteria and microbial enzyme activity, and reduced ADF and ADF digestibility.
High-Dimensional Analysis of Single-Cell Flow Cytometry Data Predicts Relapse in Childhood Acute Lymphoblastic Leukaemia
Authors: Chulián Salvador , Martínez-Rubio Álvaro , Pérez-García Víctor M. , Rosa María , Blázquez Goñi Cristina , Rodríguez Gutiérrez Juan Francisco , Hermosín-Ramos Lourdes , Molinos Quintana Águeda , Caballero-Velázquez Teresa , Ramírez-Orellana Manuel , Castillo Robleda Ana , Fernández-Martínez Juan Luis
Published: December 2020

Artificial intelligence methods may help in unveiling information that is hidden in high-dimensional oncological data. Flow cytometry studies of haematological malignancies provide quantitative data with the potential to be used for the construction of response biomarkers. Many computational methods from the bioinformatics toolbox can be applied to these data, but they have not been exploited in their full potential in leukaemias, specifically for the case of childhood B-cell Acute Lymphoblastic Leukaemia. In this paper, we analysed flow cytometry data that were obtained at diagnosis from 56 paediatric B-cell Acute Lymphoblastic Leukaemia patients from two local institutions. Our aim was to assess the prognostic potential of immunophenotypical marker expression intensity. We constructed classifiers that are based on the Fisher’s Ratio to quantify differences between patients with relapsing and non-relapsing disease. We also correlated this with genetic information. The main result that arises from the data was the association between subexpression of marker CD38 and the probability of relapse.
Thyroid Hürthle Cell Carcinoma: Clinical, Pathological, and Molecular Features
Authors: Kure Shoko , Ohashi Ryuji
Published: December 2020

Hürthle cell carcinoma (HCC) represents 3–4% of thyroid carcinoma cases. It is considered to be more aggressive than non-oncocytic thyroid carcinomas. However, due to its rarity, the pathological characteristics and biological behavior of HCC remain to be elucidated. The Hürthle cell is characterized cytologically as a large cell with abundant eosinophilic, granular cytoplasm, and a large hyperchromatic nucleus with a prominent nucleolus. Cytoplasmic granularity is due to the presence of numerous mitochondria. These mitochondria display packed stacking cristae and are arranged in the center. HCC is more often observed in females in their 50–60s. Preoperative diagnosis is challenging, but indicators of malignancy are male, older age, tumor size > 4 cm, a solid nodule with an irregular border, or the presence of psammoma calcifications according to ultrasound. Thyroid lobectomy alone is sufficient treatment for small, unifocal, intrathyroidal carcinomas, or clinically detectable cervical nodal metastases, but total thyroidectomy is recommended for tumors larger than 4 cm. The effectiveness of radioactive iodine is still debated. Molecular changes involve cellular signaling pathways and mitochondria-related DNA. Current knowledge of Hürthle cell carcinoma, including clinical, pathological, and molecular features, with the aim of improving clinical management, is reviewed.
Inflammatory Proteins HMGA2 and PRTN3 as Drivers of Vulvar Squamous Cell Carcinoma Progression
Authors: Fatalska Agnieszka , Rusetska Natalia , Bakuła-Zalewska Elwira , Kowalik Artur , Zięba Sebastian , Wroblewska Agnieszka , Zalewski Kamil , Goryca Krzysztof , Domański Dominik , Kowalewska Magdalena
Published: December 2020

Current knowledge on the biology of squamous cell vulvar carcinoma (VSCC) is limited. We aimed to identify protein markers of VSCC tumors that would permit to stratify patients by progression risk. Early-stage tumors from patients who progressed (progVSCC) and from those who were disease-free (d-fVSCC) during follow-up, along with normal vulvar tissues were examined by mass spectrometry-based proteomics. Differentially expressed proteins (DEPs) were then verified in solid tissues and blood samples of patients with VSCC tumors and vulvar premalignant lesions. In progVSCC vs. d-fVSCC tumors, the immune response was the most over-represented Gene Ontology category for the identified DEPs. Pathway profiling suggested bacterial infections to be linked to aggressive VSCC phenotypes. High Mobility Group AT-Hook 2 (HMGA2) and Proteinase 3 (PRTN3) were revealed as proteins predicting VSCC progression. HMGA2 and PRTN3 abundances are associated with an aggressive phenotype, and hold promise as markers for VSCC patient stratification. It appears that vulvovaginal microflora disturbances trigger an inflammatory response contributing to cancer progression, suggesting that bacterial rather than viral infection status should be considered in the development of targeted therapies in VSCC.
Simple Equations Method (SEsM): Algorithm, Connection with Hirota Method, Inverse Scattering Transform Method, and Several Other Methods
Authors: Vitanov Nikolay K. , Dimitrova Zlatinka I. , Vitanov Kaloyan N.
Published: December 2020

The goal of this article is to discuss the Simple Equations Method (SEsM) for obtaining exact solutions of nonlinear partial differential equations and to show that several well-known methods for obtaining exact solutions of such equations are connected to SEsM. In more detail, we show that the Hirota method is connected to a particular case of SEsM for a specific form of the function from Step 2 of SEsM and for simple equations of the kinds of differential equations for exponential functions. We illustrate this particular case of SEsM by obtaining the three- soliton solution of the Korteweg-de Vries equation, two-soliton solution of the nonlinear Schrödinger equation, and the soliton solution of the Ishimori equation for the spin dynamics of ferromagnetic materials. Then we show that a particular case of SEsM can be used in order to reproduce the methodology of the inverse scattering transform method for the case of the Burgers equation and Korteweg-de Vries equation. This particular case is connected to use of a specific case of Step 2 of SEsM. This step is connected to: (i) representation of the solution of the solved nonlinear partial differential equation as expansion as power series containing powers of a “small” parameter ϵ; (ii) solving the differential equations arising from this representation by means of Fourier series, and (iii) transition from the obtained solution for small values of ϵ to solution for arbitrary finite values of ϵ. Finally, we show that the much-used homogeneous balance method, extended homogeneous balance method, auxiliary equation method, Jacobi elliptic function expansion method, F-expansion method, modified simple equation method, trial function method and first integral method are connected to particular cases of SEsM.
Dynamics of Greenhouse Gas (GHG) Emissions in the Transportation Sector of Senegal
Authors: Tchanche Bertrand
Published: December 2020

The transportation sector of Senegal is dominated by the road subsector, which relies on fossil fuels: gasoline and diesel. Their combustion generates substances such as carbon dioxide, methane, nitrous oxide, and many others responsible for climate change, which has negative impacts on the environment, human health, and activities. This study is based on data collected from Senegal’s official reports on transport and energy, and Intergovernmental Panel on Climate Change (IPCC) greenhouse gases’ analysis methods. In the period 2000–2013, growing emissions were experienced, reaching up to 2.38 × 106 tCO2-eq in 2013. The aging vehicle fleet (~20 years old on average), made up of light-duty vehicles (around 85%), a fast-growing number of imported cars, and the predominance of diesel engines (around 59%) are the aggravating factors. Beyond climate change, other gaseous substances resulting from the combustion of fuels such as carbon monoxide (CO), sulfur oxide (SO2), and particle matters (PMs) contribute to the deterioration of the outdoor air quality. Therefore, it is becoming urgent to monitor the evolution of these emissions and take appropriate measures to reduce their concentrations in the atmosphere. The Government of Senegal has taken a step forward through the modernization of transport infrastructure, and the creation of a center dedicated to the monitoring of outdoor air quality (Centre de Gestion de la Qualité de l’Air—CGQA) and a center in charge of the technical control of vehicles (Centre de Contrôle Technique des Véhicules Automobiles—CCTVA) in Dakar, but much remains to be done.
Impact of Trade and Financial Globalization on Renewable Energy in EU Transition Economies: A Bootstrap Panel Granger Causality Test
Authors: Bayar Yilmaz , Sasmaz Mahmut Unsal , Ozkaya Mehmet Hilmi
Published: December 2020

The globalized world has experienced significant environmental degradation together with raising global production and population. In this context, the employment of renewable energy use has become crucial for a sustainable environment and development. In the research, the mutual causality among renewable energy, trade and financial globalization, real GDP per capita, and CO2 emissions in EU transition economies experiencing the integration with global economy was explored through bootstrap panel Granger causality test for the period of 1995–2015. The causality analysis revealed a unilateral causality from trade globalization to renewable energy in Estonia, Latvia, and Slovenia, and from renewable energy to trade globalization in Croatia and Lithuania. However, no significant causality between financial globalization and renewable energy was revealed. On the other side, a unilateral causality from CO2 emissions to renewable energy in Lithuania and Slovenia, and from renewable energy to CO2 emissions in Czechia, Hungary, and Latvia and a reciprocal causality between renewable energy to CO2 emissions in Romania and Slovakia and a unilateral causality from real GDP per capita to renewable energy in Czechia, Romania, and Slovenia was discovered in the causality analysis.
Attitude toward and Awareness of Renewable Energy Sources: Hungarian Experience and Special Features
Authors: Szakály Zoltán , Balogh Péter , Kontor Enikő , Gabnai Zoltán , Bai Attila
Published: December 2020

The current paper analyzes the awareness of renewable energy sources (RES), the relationship between self-reported and actual knowledge, and the correlation among the knowledge of renewable energy sources, the characteristic stereotypes, and the typical attitude of different social groups to energy, comparing them with international experience. A nationwide representative questionnaire-based survey was carried out involving 1002 people in Hungary in 2019. Better education, a higher income, an active white-collar profession, and a health- and environment-conscious approach to life (LOHAS (lifestyle of health and sustainability) segment) are definitely an advantage when it comes to knowledge of renewable energy sources. No significant relationship was detected in terms of age; however, in cluster formation, young people were typically found to be better informed. Overall, the actual knowledge of the Hungarian respondents is more favorable than the self-reported one, and the basic level of knowledge of energy sources in the case of wind and hydropower exceeds international experience. The social factors of better knowledge essentially correspond to the international trends; however, regarding firewood, solar, and wind energy, the average Hungarian has certain false stereotypes that can be considered typical. The assessment of convenience and that of environmental aspects are almost the same.