Properties of the antimicrobial activity of polyhexanide drugs against the dominant pathogens of wound infection in Ukraine: modern realities
Authors:   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Nazarchuk O.A. , Rusak P.S. ,   Shupyk National Healthcare University of Ukraine, Kyiv, Chornopyshchuk R.M. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Denysko T.V. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Vovk I.M. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Grebeniuk D.I. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Skrypka A.S. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Chornopyshchuk N.P. ,   National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Published: December 2024

The treatment of acute and chronic wounds with critical colonization by wound pathogens remains a serious problem, which is becoming more and more threatening every year due to the global problem of antibiotic resistance.
Aim - to study the antimicrobial activity of modern antiseptic Polyhexanide and antimicrobial biomaterials based on it against most common pathogens of wound infections.
Materials and methods. Antimicrobial activity of polyhexanide solution were studied against MLS -resistant clinical isolates of S. aureus, MDR- strains of E. coli, clinical XDR-isolates of K. pneumoniae, P. aeruginosa, and A. baumannii as well as referent strains S. aureus ATCC 25923, E. coli ATCC 25922, K. pneumoniae ATCC 708603, P. aeruginosa ATCC 27853, A. baumannii ATCC ВАА-747. MIC and MBC of polyhexanide were detected by standard dilution method; bactericidal index of antiseptic activity (BC IAA) for 0.1% polyhexamethylen-biguanide solution for wound irrigation (PHMB) was calculated. The bioactivity of wound antimicrobial dressings with polyhexamethylenguanidyn-hydrochoridi (PHMB-HCl) and PHMB was determined by standard disk diffusion method; growth inhibition zones (GIZ) were measured and compared.
Results. MLS-resistant strains of S. aureus were the most susceptible to the action of the antiseptic: the MIC and MBC of PHMB were on average 22.69±4.6 μg/ml and 40.55±8.57 μg/ml, respectively. The MIC values against S. aureus was lower than those against E. coli by 1.97 times, against K. pneumoniae – by 1.78 times, against A. baumannii – by 2.97, against P. aeruginosa – 3.9 times. The MBC of PHMB against clinical strains of S. aureus had the lowest values and differed significantly from those against E. coli by 2.21 times, K. pneumoniae by 1.99 times, A. baumannii by 3.4 times, P. aeruginosa by 4.52 times. The bactericidal index of antiseptic activity (BC IAA) of PHMB had the highest values in relation to clinical strains of S. aureus (BC IAA=24.7), K. pneumoniae (BC IAA=12.4) and E. coli (BC IAA=11.2). Wound dressings PHMB-HCl and PHMB demonstrated high antimicrobial properties against reference and clinical strains of S. aureus, E. coli, K. pneumoniae. Reference and clinical strains of P. aeruginosa were the least susceptible to PHMB-based wound dressings.
Conclusions. Polyhexanide antiseptic solution for wound irrigation and polyhexanide-containing dressings with PHMB and PHMB-HCl have the high antimicrobial properties in relation to the most common types of wound pathogens.
No conflict of interests was declared by the authors.