bluuebunny/crossref_metadata_2025 huggingface dataset:
Water hardness influenced reproductive dynamics in two freshwater fish species; Poecilia reticulata and Betta splendens
Authors: Krishnakumar Abarna , Patrick Anton , Jayawardena Uthpala
Published: June 2020

Objective:
Hardness of water in the form of CaCO3 affect reproductive potential in various fish species, differently. The study evaluates the effect of water hardness on growth and reproduction of two aquarium fishes, Poecilia reticulata (Ovo-viviparous sp.) and Betta splendens (Oviparous sp.) by growing them under 150 (control), 320, 540 and 900 ppm CaCO3 levels in semi natural aquaria.
Results:
Growth increased with increasing water hardness, reporting a significant growth of P. reticulata (p = 0.005) at 900 ppm. Similarly, the reproductive potential of P. reticulata was improved significantly, recording the highest fecundity (16.22 ± 3.90) and Gonadosomatic Index (GSI-2.48 ± 0.597) at 900 ppm. However, in B. splendens water hardness adversely affected the reproduction by reporting a significantly low hatchability and disturbed bubble nests at 900 ppm, compared to the largest bubble nest formed at the control (108.58 ± 16.19 cm2). Thus, study revealed differential effects of water hardness on reproductive potential of the test species, by increasing the potential of P. reticulata while decreasing that of B. splendens. Though larval survival was affected in both species, larval growth was improvement significantly in P. reticulata at 900 ppm level. Understanding reproductive dynamics in natural waters are crucial for propagation and conservation perspectives of freshwater fishes.
Comparation of Laparoscopic Intersphincteric Resection and Abdominoperineal Resection for Ultra-Low Rectal Cancer Patients - a Retrospective Analysis, and a Meta-analysis of Oncology Outcome
Authors: Mo Xianwei , Wang Wentao , Qin Haiquan , Lai Hao , Huang Zigao , Liu Jungang , Zuo Hongqun , Lin Yuan , Tang Weizhong
Published: June 2020

Purpose

The aim of the study is to evaluate the surgical and oncology outcomes between laparoscopic Intersphincteric Resection (LISR) and laparoscopic-assisted Abdominoperineal Resection (LARC) for ultra-low rectal cancer patients by using a retrospective analysis, and a meta-analysis of the literature was carried out to further validate the oncology outcome.
Patients and methods:

Between April 2014 and December 2015, a total of 38 rectal cancer patients who underwent LISR and 41 LARC patients were enrolled in this study. The comparison between the groups was based on clinicopathological characteristics and surgical outcomes. Meta-analysis of published studies, exploring oncology outcome of between LISR and LARC, was carried out using STATA 12.0 software.
Results

Operating time, blood loss, length of hospital stay, and postoperative complication rates was similar between LISR group and LARC group; Patients undergoing LISR also had a similarly 5-year local recurrence and overall survival rate with LAPR. Meta-analysis showed that five studies, which included a total of 791 patients were final involved for this analysis. Comparing APR, ISR patients obtain similarly 5-years recurrence rate and 5-years survival rate.
Conclusion

This study suggests that LISR is as technically feasible, safe, and effective as LARC for treating patients with low rectal cancer. Additional high-powered randomized trials are needed to determine whether LISR truly offers any advantages.
Leadership Perspectives on Learning Health Systems: a qualitative study
Authors: Enticott Joanne , Braaf Sandy , Johnson Alison , Jones Angela , Teede Helena
Published: June 2020

Background: Integrated utilisation of digital health data has the power to transform healthcare to deliver more efficient and effective services, and the learning health system (LHS) is emerging as a model to achieve this. The LHS uses routine data from service delivery and patient care to generate knowledge to continuously improve healthcare. The aim of this project was to explore key features of a successful and sustainable LHS to inform implementation in an Academic Health Science Centre context.Methods: We purposively identified and conducted semi-structured qualitative interviews with leaders, experienced in supporting or developing data driven innovations in healthcare. A thematic analysis using NVivo was undertaken.Results: Analysis of 26 interviews revealed five themes thought to be integral in an effective, sustainable LHS: (1) Systematic approaches and iterative, continuous learning with implementation into healthcare contributing to new best-practice care; (2) Broad stakeholder, clinician and academic engagement, with collective vision, leadership, governance and a culture of trust, transparency and co-design; (3) Skilled workforce, capability and capacity building; (4) Resources with sustained investment over time and; (5) Data access, systems and processes being integral to a sustainable LHS. Conclusions: This qualitative study provides insights into the elements of a sustainable LHS across a range of leaders in data-driven healthcare improvement. Fundamentally, an LHS requires continuous learning with implementation of new evidence back into frontline care to improve outcomes. Structure, governance, trust, culture, vision and leadership were all seen as important along with a skilled workforce and sustained investment. Processes and systems to optimise access to quality data were also seen as vital in an effective, sustainable LHS. These findings will inform a co-designed framework for implementing a sustainable LHS within the Australian healthcare and Academic Health Science Centre context. It is anticipated that application of these findings will assist to embed and accelerate the use of routine health data to continuously generate new knowledge and ongoing improvement in healthcare delivery and health outcomes.
Evaluation of The Standard Procedure for the Treatment of Periprosthetic Joint Infections (PJI) in Germany - Results of a Survey within the EndoCert Initiative
Authors: Rimke Christina , Enz Andreas , Bail Hermann Josef , Heppt Peter , Kladny Bernd , von Lewinski Gabriela , Lohmann Christoph H. , Osmanski-Zenk Katrin , Haas Holger , Mittelmeier Wolfram
Published: June 2020

Background: The PJI is a severe complication in the field of arthroplasty.Despite the rising number of primary joint replacements, no therapeutic standard has been established for the treatment of PJI yet.Methods: A survey on the principles of treatment of PJI in Germany wasconducted. A total of 515 EndoProthetikZentren were included, resulting in a response rate of 100%.Results: For early infections 97.6% of the centers use DAIR. A one-stage exchange was implemented by less than 50% of the centers. If implemented, this treatment entails a prior selection of patients for a successful treatment. The two-stage exchange is performed in all centers, and most centers proceed with the implantation of a cemented spacer between stages. 75% of the centers proceed with a center-based concept for the treatment of PJI.Conclusion: The rising number of primary joint replacements underlines the importance of developing standards and harmonized definitions for the treatment of PJI. These standards have not yet been fully achieved. The most relevant treatment options in Germany are displayed. The two-stage revision with a cemented spacer is the most widely implemented treatment option in Germany. This summary of principles could serve as a basis for the development of standardized treatment guidelines and definitions.
Short- and Long-Term Outcomes of Laparoscopic Surgery for Colorectal Cancer in the Elderly Aged Over 80 Years Old Versus Non-Elderly: A Retrospective Cohort Study
Authors: Ueda Yoshitake , Shiraishi Norio , Kawasaki Takahide , Akagi Tomonori , Ninomiya Shigeo , Shiroshita Hidefumi , Etoh Tsuyoshi , Inomata Masafumi
Published: June 2020

Background Recently, colorectal cancer has increased in elderly patients worldwide, with laparoscopic colorectal surgery increasing in elderly patients with colorectal cancer. However, whether laparoscopic colorectal surgery is an optimal procedure for colorectal cancer in the elderly remains unclear.Objective This study aimed to verify safety and curability of laparoscopic colorectal surgery in elderly patients ≥80 years old.Methods Patients undergoing curative colorectal surgery from 2006 to 2014 were enrolled and classified into the laparoscopic surgery in elderly patients aged ≥80 years (LAC-E) group, open surgery in elderly patients (OC-E) group, and laparoscopic surgery in non-elderly patients (LAC-NE) group. Short- and long-term outcomes were compared between these groups.Results The LAC-E, OC-E, and LAC-NE groups comprised 85, 25, and 358 patients, respectively. Intraoperative blood loss and incidence of postoperative complications were significantly lower in the LAC-E versus OC-E group (97 vs. 440 mL, p<.01 and 14% vs. 32%, p<.05, respectively). Long-term outcomes were not different between these two groups. Operation time was significantly shorter in the LAC-E versus LAC-NE group (249 vs. 288 min, p<.01). Intraoperative blood loss and postoperative complications were similar between the groups. Although the 5-year overall survival rate in the LAC-E group was lower than that in the LAC-NE group (64% vs. 80%, p<.01), there was no difference in 5-year disease-specific survival between the groups.Conclusions Laparoscopic colorectal surgery is technically and oncologically safe for colorectal cancer in the elderly as well as the non-elderly and can be an optimal procedure for colorectal cancer in the elderly.
Mid-late pregnancy maternal glycolipids in gestational diabetes mellitus (GDM) fluctuated in glycosylated hemoglobin levels of 5.5%-6.4% associated with umbilical cord blood of amino acid and carnitine profiles: a nested case-cohort observational study
Authors: yuhang long , Chen Le , Yang Yaochao , Liu Wei , Zhang Hanxi , Chen WuJuan , Jiang Fangfang , Hong Ting , Wang Jianying , Chen Yao , Cao Jianyong , Xie Dan , Su Zhaojuan
Published: June 2020

Background

To explore the relationship between maternal-neonatal clinical data and the changes of cord blood amino acid and carnitine levels between pregnant women with gestational diabetes mellitus (GDM) when glycosylated hemoglobin levels of 5.5%-6.4% excluded diabetic medicine as GDMa group and the control group.
Methods

In all, 312 qualified participants were recruited with permission from the ethical department of the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two groups (GDMa and non-GDM) after adjusting for maternal age and body mass index (BMI) between the 1 June 2017 and 30 April 2020. All maternal-neonatal data were collected and analysed at the centre.
Results

Interestingly, glycine in cord blood was not only significantly different between groups(594.19 vs 407.45, P < 0.001), but also associated among those pregnant women in GDM when glycosylated hemoglobin levels of 5.5%-6.4% through the standard of diet control and exercise (b = 0.449, P = 0.010). Meanwhile, neonatal hypoglycemia had the positive association with GDM monitoring glycosylated hemoglobin levels of 5.5%-6.4% (P = 0.031, OR 5.77 95%CI[1.18, 28.36]), but none of correlations with umbilical cord blood of amino acids and carnitines.
Conclusions

The study identifies some differences and relationships in maternal-neonatal data only when GDMa group, glycosylated hemoglobin levels of 5.5%-6.4% without diabetic medicine, compared with the control group which adjusted by age and BMI. Particularly, umbilical cord blood of glycine levels is related to the diabetic status of glycosylated hemoglobin 5.5%-6.4% in GDM during pregnancy and could be potential diabetic mechanism by maternal-fetal interface.
Time Trends in Comorbidity and Management of Hypertension and Diabetes: A 15-year Nationwide Longitudinal Study of 18,380 Adults in China
Authors: Li Yixuan , Sun Xiaomin , Wei Junxiang , Wu Jing , Wang Youfa
Published: June 2020

Background: To examine time trends in comorbidity of hypertension and type 2 diabetes (T2DM) and their diagnosis, treatment, and management in China during 2000-2015 and study factors associated with these outcomes. Methods: Longitudinal data collected from the China Health and Nutrition Survey (CHNS) during 2000-2015 were analyzed. Of the 28,204 adults, 832 had both hypertension and T2DM were selected. Average systolic blood pressure (SBP) and diastolic blood pressure (DBP) and hypertension prevalence among T2DM patients, and treatment and control of hypertension and T2DM among patients with both conditions were examined for all and by sex and weight status. Mixed effects models assessed the associations. Results: From 2000 to 2015, among patients with T2DM, hypertension prevalence dropped from 88.4% to 83.0% and BPs decreased (P < 0.05). Men and overweight/obese patients had greater decreases in hypertension prevalence and DBP, while women had a larger decrease in SBP than men. Over time, among patients with both hypertension and T2DM, rates of hypertension treatment (45.3% to 57.7%), hypertension control (3.0% to 10.9%) and T2DM treatment (90.0% to 95.6%) increased, but were still very low except for T2DM treatment (all P < 0.001). Age, gender, smoking, drinking, household income, education, residence, and weight status were significantly associated with prevalence, treatment, and control of hypertension, and T2DM treatment among patients with both hypertension and T2DM. Conclusion: Rates of hypertension treatment and control among patients with both hypertension and T2DM have improved in recent years, but were still low.
Nomogram for Predicting Survival in Mucinous Adenocarcinoma of Prostate
Authors: Li Jiakun , Qiu Shi , Cui Xi-nan , Jin Kun , Zheng Xiaonan , Tu Xiang , Ge Liming , Yang Lu , wei qiang
Published: June 2020

Background: Few studies on predicting survival in patients with histology of mucinous adenocarcinoma of the prostate have been done.Objective: Nomogram is a mathematical model in which various important factors are combined to predict a specific end point. The purpose of this study was to evaluate the prognostic value of clinical factors in patients with mucinous adenocarcinoma and to construct nomogram to predict the survival rate. Design,Setting,and Participants: A nomogram was designed to predict the survival rate of 356 patients with invasive ductal carcinoma selected from the surveillance, epidemiology and final results (SEER) database. Univariate and multivariate models of the cohort were done firstly.And then a model of nomogram that included age, race, grade, stage M and chemotherapy were constructed to predict the 3-year and 5-year survival of prostate cancer patients with histology of mucinous adenocarcinoma. Based on different time, we designed two nomograms.Results and limitations: After the discrimination and calibration, C-index was 0.8138. The specificity was 86.12%, sensitivity was 55.89% for 3-year nomogram . Its survival was 91.49% and AUC was 0.7467. The specificity was 82.42%, sensitivity was 54.83% for 5-year nomogram. Its Survival was 86.73% and AUC was 0.7555. Respectively, which indicated relative good discrimination of the nomogram. Conclusions: This clinical model shows a high ability to predict the survival rate of mucinous adenocarcinoma patients with histologically prostate cancer, making this model a novel and attractive model for predicting the survival rate of patients.
Genetic Analysis Reveals Unique Characteristics of Plasmodium Falciparum Parasite Populations in Haiti
Authors: Daniels Rachel , Chenet Stella , Rogier Eric , Lucchi Naomi , Herman Camelia , Pierre Baby , Lemoine Jean Frantz , Boncy Jacques , Wirth Dyann , Chang Michelle , Udhayakumar Venkatachalam , Volkman Sarah
Published: June 2020

Background: With increasing interest in eliminating malaria from the Caribbean region, Haiti is one of the two countries on the island of Hispaniola with continued malaria transmission. While the Haitian populace remains at risk for malaria, there are a limited number of cases annually, making conventional epidemiological measures such as case incidence and prevalence of potentially limited value for fine-scale resolution of transmission patterns and trends. In this context, genetic signatures may be useful for the identification and characterization of the Plasmodium falciparum parasite population in order to identify foci of transmission, detect outbreaks, and track parasite movement to potentially inform malaria control and elimination strategies. Methods: This study evaluated the genetic signals based on analysis of 21 single-nucleotide polymorphisms (SNPs) from 462 monogenomic (single-genome) P. falciparum DNA samples extracted from dried blood spots collected from malaria-positive patients reporting to health facilities in three southwestern Haitian departments (Nippes, Grand’Anse, and Sud) in 2016. Results: Assessment of the parasite genetic relatedness revealed evidence of clonal expansion within Nippes and the exchange of parasite lineages between Nippes, Sud, and Grand'Anse. Furthermore, 437 of the 462 samples shared high levels of genetic similarity­­—at least 20 of 21 SNPS—with at least one other sample in the dataset. Conclusions: These results revealed patterns of relatedness suggestive of the repeated recombination of a limited number of founding parasite types without significant outcrossing. These genetic signals offer clues to the underlying relatedness of parasite populations and may be useful for the identification of the foci of transmission and tracking of parasite movement in Haiti for malaria elimination.
Acute Kidney Injury in COVID-19: clinical outcomes and risk factors
Authors: Jin Kui , Xie Tuxiu , Seery Sam , Ye Lu , Jiang Jie , Yang Weize , Luo Xiaomin , Wei Jie , Yin Lu , Fu Yangyang , Yu Shanshan , Zhang Lili , Xu Jun , lyu Jingjun
Published: June 2020

Background:Understanding of the incidence and effects of acute kidney injury (AKI) in patients diagnosed with COVID-19 is limited. The purpose of this study was to examine risk factors and related outcomes associated with AKI among patients diagnosed with COVID-19. Method: This is a retrospective cohort study of patients diagnosed with COVID-19 associated-pneumonia admitted to a tertiary hospital in Wuhan between January to February 2020. AKI was defined and staged according to the Kidney Disease: Improving Global Outcome (KDIGO) classification criteria. Cox’s multivariate regression and logistic regression modelling were used to assess the effects of AKI on hospital mortality and risk factors associated with occurrence of AKI. Primary outcomes were risk-adjusted in-hospital mortality.Results:342 patients were finally enrolled in this study. AKI occurred in 13.4% (n = 46), among them 7.0% (n = 24) developed stage 1AKI, and 6.4% (n = 22) developed stage 2 - 3 AKI. Overall 26.9% (n = 92) died during hospitalization. Among them 19.3% (57/296) of the non-AKI patients died, 62.5%(15/24) of stage 1 AKI patients, and 90.9% (20/22) of stage 2 - 3 AKI patients died. AKI was strongly associated with mortality (HR 2.52; 95% CI, 1.59-3.96; p＜0.001). Further analysis shows that progression to AKI stage 2 - 3 doubles the hazard ratio for death. Age, leukocytes number, fibrinogen concentration, C-reative protein level, and severity of pneumonia at admission were independent risk factors associated with the development of AKI. Conclusion:Acute kidney injury is common among hospitalized COVID-19 patients and strongly associated with increased mortality, early detection and prevention of the progression of AKI may be critical to reduce mortality of these patients.
Lymphocytopenia and neutrophilia deteriorate at the lowest oxygenation index timepoint in COVID-19 patient
Authors: Hong Wei-jun , Xu Yi-wen , Li Wei-ling , Xu Chun-qiang , Hu Xiao-fei , Zhang Mei-xian , Xu Hui-hui , Wang En , Ke Shao-fa , Jin Xiao-ping , Wu Gang , Liu Xi-chang
Published: June 2020

BackgroundCoronavirus disease 2019 (COVID-19) spread throughout the world and caused hundreds of thousands of infected people to death. However, the pathogenesis of severe acute respiratory syndrome coronavirus-2 (SARS COV-2) is poorly understood. The objective of this study is to retrospectively explore the pathogenesis of COVID-19 from clinical laboratory findings, taking disease progression into account.MethodsA single-centered, retrospective study was carried out, which included moderate (n=76) and severe COVID-19 cases (n=22). The difference of laboratory findings from blood routine examination and hepatorenal function test were retrospectively evaluated between the state of moderate and severe. The disease progression was indicated by oxygenation index.ResultsAge is a risk factor for disease progression from moderate to severe. Lymphocytopenia, neutrophilia, liver and kidney function decreasement occurred in severe patients on admission, compared with moderate patients. Lymphocytopenia and neutrophilia deteriorated at the lowest oxygenation index timepoint in the severe patients. And the oxygenation index was associated with ratio of lymphocyte and neutrophil in COVID-19 patients.ConclusionsLymphocytopenia and neutrophilia, which deteriorate in the progression of severe patients, are the main pathogenesis of COVID-19. More measures need to be taken to control lymphocytopenia and neutrophilia in severe COVID-19. Oxygenation index presented potentiality as predictor on the progression of COVID-19.
Modeling of spreading of the novel coronavirus based in the stochastic dynamic
Authors: Lima Leonardo S.
Published: June 2020

In this paper, one proposes a stochastic model based on Itô diffusion as mathematical model for time evolution of novel cases N(t) of the SARS-CoV-2 (COVID-19) in each day t. I propose a correspondent stochastic differential equation (SDE) analogous to classical differential equation for epidemic growing for some diseases as smallpox and typhoid fever. Furthermore, we made an analysis using the Fokker-Planck equation giving an estimating of the novel cases in the day t as the mean half-width of the distribution P(N,t) of novel cases. My results display that the model based on Itô diffusion fits well to the results supported by healthy Brazilian agencies due to large uncertainly in the official data generated by the low number of tests realized generating so a strong randomness in the official data.
A Network Pharmacology Based Analysis of The Potential Mechanisms of Compound Danshen Dripping Pill for Treatment of Diabetic Retinopathy
Authors: Zhang Xinyue , Wang Lizhen , Huang Xiaoying , Zheng Yanlin
Published: June 2020

Background: Diabetic retinopathy (DR) has become a worldwide concern in recent years because of the high prevalence and vision-threat. The limited therapies have been in stark contrast to its high prevalence. On top of that, almost all the treatments, like laser, are applied only at the end stage of this disease. However, with the development of network pharmacology, a traditional Chinese medicine (TCM), Compound Danshen Dripping Pill (CDDP), may bring some new insights into the early intervention of DR. Methods: The active compounds and potential targets of CDDP and DR-related targets were collected from the TCMSP, UniProt, GeneCards and OMIM databases. And protein-protein interactions (PPI) information was achieved from the STRING database. The gene enrichment analysis of GO and KEGG was carried out by “clusterProfiler” in R software. The collected data was then used to form network maps of compound-target and PPI, or visualized by the software of Cytoscape. Results: 54 compounds and 50 targets were identified for CDDP against DR. Among the predicted effective compounds, 29 tanshinones from Radix Salviae (Danshen) were identified. And the core targets might be estrogen receptor (ESR1), androgen receptor (AR), caspase-3 (CASP3), Interleukin-6 (IL6) and vascular endothelial growth factor A (VEGFA) in 50 targets. There were 266 significantly correlated biological processes such as steroid hormone response, oxidative stress and apoptosis enriched out. Besides, The 50 targets significantly participate in 97 pathways and mainly enriched in advanced glycation end products (AGE)-receptor for advanced glycation end products (RAGE), fluid shear stress and atherosclerosis, apoptosis and VEGF signal pathway et al.Conclusions: The mechanisms of CDDP for treating DR may involve multi-compound, multi-target and multi-pathway synergistic effects. It may participate in the regulation of steroid hormone response, oxidative stress, apoptosis and hemodynamics in diabetic retina. Tanshinones and luteolin from Radix Salviae were probably responsible for the effectiveness of CDDP on DR.
Performance of the QPLEXTM Alz Plus Assay, A Novel Multiplex Kit for Screening Cerebral Amyloid Deposition
Authors: Park Jong-Chan , Jung Keum Sim , Kim Jiyeong , Jang Ji Sung , Kwon Sunghoon , Byun Min Soo , Yi Dahyun , Byeon Gihwan , Jung Gijung , Kim Yu Kyeong , Lee Dong Young , Han Sun-Ho , Mook-Jung Inhee
Published: June 2020

Background: Alzheimer's disease (AD) is an irreversible neurodegenerative disease characterized by the hallmark finding of cerebral amyloid deposition. Many researchers have tried to predict the existence of cerebral amyloid deposition by using easily accessible blood plasma samples, but the effectiveness of such strategies remains controversial. Methods: We developed a new multiplex kit, the QPLEXTM Alz plus assay kit, which uses proteomics-based blood biomarkers to prescreen for cerebral amyloid deposition. A total of 300 participants who underwent Pittsburgh compound B (PiB)-positron emission tomography (PET) which allows imaging of cerebral amyloid deposition were included in this study. We compared the levels of QPLEXTM biomarkers between patients who were classified as PiB-negative or PiB-positive, regardless of their cognitive function. Logistic regression analysis followed by receiver operating characteristic (ROC) curve analysis was performed. The kit accuracy was tested using a randomized sample selection method.Results: The results obtained using our assay kit reached 89.1% area under curve (AUC) with 80.0% sensitivity and 83.0% specificity. Further validation of the QPLEXTM Alz plus assay kit using a randomized sample selection method showed an average accuracy of 81.5%.Conclusions: Our QPLEXTM Alz plus assay kit offers a highly effective way to predict cerebral amyloid deposition through a convenient blood test, and should enable easy prediction of AD in daily life.
A graph-based network for predicting chemical reaction pathways in solid-state materials synthesis
Authors: McDermott Matthew J. , Dwaraknath Shyam S. , Persson Kristin A.
Published: June 2020

Accelerated synthesis of inorganic materials remains a significant challenge in the search for novel, functional materials. Many of the chemical principles which enable "synthesis by design" in synthetic organic chemistry do not exist in solid-state chemistry, despite extensive computed/experimental thermochemistry data. We present a chemical reaction network model constructed from thermochemistry databases that captures features of the thermodynamic phase space which synthesis reactions traverse. Directed edges in the network are assigned weights via a transformation that maps reaction parameters to costs. We devise a computationally tractable approach for suggesting likely reaction pathways via application of pathfinding algorithms and linear combination of lowest-cost paths in the network. We demonstrate initial success of the reaction network in predicting a complex metathesis reaction pathway toward yttrium manganese oxide YMnO3. The reaction network presents new opportunities for enabling reaction pathway prediction, rapid iteration between experimental/theoretical results, and ultimately, control of synthesis of solid-state materials.
Defining the Extracellular Matrix of Rhabdomyosarcoma
Authors: Lian Xiaolei , Bond J. Steffan , Bharathy Narendra , Boudko Sergei P. , Pokidysheva Elena , Shern Jack F. , Lathara Melvin , Settelmeyer Teagan , Cleary Megan M. , Bajwa Ayeza , Sriniv Ganapati , Hartley Christopher P. , Bächinger Hans Peter , Mansoor Atiya , Gultekin Sakir H. , Berlow Noah E. , Keller Charles
Published: June 2020

Background: Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of childhood with a propensity to metastasize. Current treatment for patients with RMS includes conventional systemic chemotherapy, radiation therapy and surgical resection; nevertheless, little to no improvement in long term survival has been achieved in decades – underlining the need for target discovery and new therapeutic approaches to targeting tumor cells or the tumor microenvironment.Methods:To evaluate cross-species sarcoma extracellular matrix production, we have used murine models which feature knowledge of the myogenic cell-of-origin. With focus on the RMS/undifferentiated pleomorphic sarcoma (UPS) continuum, we have constructed tissue microarrays of 48 murine and 4 human sarcomas to analyze expression of 7 different collagens, fibrillins and collagen-modifying proteins, with cross-correlation to RNA deep sequencing.Results:We have uncovered that RMS produces increased expression of type XVIII collagen alpha 1 (COL18A1), which is clinically associated with decreased long-term survival. We have also identified significantly increased RNA expression of COL4A1, FBN2, PLOD1 and PLOD2 in human RMS relative to normal skeletal muscle.Conclusion:These results complement recent studies investigating whether soft tissue sarcomas utilize collagens, fibrillins and collagen-modifying enzymes to alter the structural integrity of surrounding host extracellular matrix/collagen quaternary structure resulting in improved ability to improve the ability to invade regionally and metastasize, for which therapeutic targeting is possible.
Identification of the active substances and mechanisms of ginger for the treatment of colon cancer based on network pharmacology and molecular docking
Authors: Zhang MengMeng , Wang Dan , Lu Feng , Zhao Rong , Ye Xun , He Lin , ai li , Wu ChunJie
Published: June 2020

Background: Colon cancer is increasing recently but the high cost and adverse side effects experienced always leads to treatment drop out. Zingiber officinale, commonly known as ginger, is a popular herbal medicine and this study was aimed to identify the active compounds from ginger and to investigate its anti-cancer mechanisms through network pharmacology construction. Results: Ginger compounds were discerned through the TCMSP, which were filtered by the metrics of oral bioavailability and drug likeness, and its related targets were searched. After that, the targets interacting with colon cancer were collected using Genecards, OMIM, and Drugbank databases. Six potential active compounds, 288 interacting targets in addition to 1356 disease-related targets were collected, of which 114 intersection targets were obtained. The PPI network showed that 32 targets including SRC, PIK3R1, and TP53 were identified as key targets. These targets were mainly associated with the biological processes like transmembrane receptor protein tyrosine kinase signaling pathway, regulation of cellular protein localization, cellular response to oxidative stress. KEGG enrichment manifested that ginger probably produced preventive effects against colon cancer by regulating significant signaling pathway like pathway in cancer, hepatitis B, and estrogen signaling pathway. TP53, HSP90AA1, MAPK8, JAK2, CASP3, and ERBB2 could be viewed as the most potential target proteins, which were validated by molecular docking simulation.Conclusion: This study demonstrated the multi-component, multi-target, and multi-pathway characteristics of ginger, providing novel insight for ginger compounds developed as new drug for anti-colon cancer.
Recombinant Bacteriophage LysKB317 Endolysin Mitigates Lactobacillus Infection of Corn Mash Fermentations
Authors: Lu Shao- Yeh , Bischoff Kenneth M. , Rich Joseph O. , Liu Siqing , Skory Christopher D.
Published: June 2020

Background Commercial ethanol fermentation facilities traditionally rely on antibiotics for bacterial contamination control. Here we demonstrate an alternative approach to treat contamination using a novel peptidoglycan hydrolase (LysKB317) isolated from a bacteriophage, EcoSau. This endolysin was specially selected against Lactobacillus strains that were isolated as contaminants from a fuel ethanol plant. The LysKB317 gene was recombinantly expressed in Escherichia coli as a 33 kDa purified enzyme. Results In turbidity reduction assays, the recombinant enzyme was subjected to a panel of 32 bacterial strains and was active against 28 bacterial strains representing one species of Acetobacter, eight species of Lactobacillus, one species of Pediococcus, three species of Streptococcus, and one species of Weissella. The activity of LysKB317 was optimal around pH 6, but it has broad activity and stability from pH 4.5 – 7.5 up to at least 48 h. Maximum activity was observed at 50°C up to at least 72 h. In addition, LysKB317 was stable in 30% ethanol up to at least 72 h. In experimentally infected corn mash fermentations, 1 µM endolysin reduced bacterial load by 4-log fold change, while 0.01 µM reduced bacteria by 2-log fold change. Concentration of fermentation products (ethanol, residual glucose, lactic acid, and acetic acids) for infected cultures treated with ≥ 0.01 µM LysKB317 were similar to uncontaminated controls. Conclusion Exogenously added LysKB317 endolysin is functional in conditions typically found in fuel ethanol fermentations tanks and may be developed as an alternative to antibiotics for contamination control during fuel ethanol fermentations.
Comparison of Obesity-Related Parameters as Predictors of High Pulse Wave Velocity in Middle-Aged and Elderly People in China: A Multicenter Cross-Sectional Community-Based Study
Authors: Sun Ting , Xie Hui , Ma Zuchang
Published: June 2020

Background: The association between fat-related parameters and occurrence of high pulse wave velocity (PWV) in Chinese middle-aged and elderly people is unknown, especially when booy composition indicators are compared.Methods: A total of 3219 middle-aged and elderly subjects who were recruited from 6 community health service centers located in Hefei, Bengbu, and Chuzhou met the inclusion criteria and had valid data. E-health promotion system was used to collect health basic data, and brachial-ankle pulse wave (baPWV) and body composition of each subject were measured. Partial correlation and binary logistic regression analyses were performed to identify associations between fat-related parameters and high PWV, and receiver operating characteristic curves were analyzed for optimal cutoff values and predictive capacity for high PWV.Results: The highest partial correlation coefficients (adjusted for age) for waist-to-height ratio (WHtR) were in middle-aged women and elderly men (range, 0.1-0.31); and that for waist circumference (WC) were in elderly women and middle-aged men (range, 0.12-0.29). WHtR explained the largest proportion of variation for dependent variables, with an R2 value ranging from 0.088 to 0.216 in Model 1; the beta of WC was slightly higher than that of WHtR in elderly women in Models 2 and 3. The predictive capacities of these parameters were lower in men. The area under the receiver operating characteristic curve was higher for WHtR (0.573–0.693) than for the other parameters in both men and women, with optimal cutoff values of 0.51-0.54.Conclusions: WHtR and WC may be useful for community-based screening of women ≥40 years as a secondary preventative measure for high PWV. These 2 parameters can be used in conjunction with others (eg., body composition index) to predict the risk of high PWV based on region, age, and sex.
Generation and Characterization of Skeletal Muscle Cell based Model for GNE Myopathy: Implications in Actin Dynamics
Authors: Devi Shamulailatpam Shreedarshanee , Yadav Rashmi , Mashangva Fluencephila , Chaudhary Priyanka , Sharma Shweta , Arya Ranjana
Published: June 2020

UDP-N-acetyl glucosamine-2 epimerase/ N-acetyl mannosamine kinase (GNE) catalyzes key enzymatic reactions in the biosynthesis of sialic acid. Mutation in GNE gene causes GNE-Myopathy characterized by adult onset muscle weakness and degeneration. GNE is involved in different cellular processes like adhesion, apoptosis, ER stress and autophagy. Lack of appropriate model system limits drug and treatment options for GNE Myopathy as GNE knock out was found to be embryonically lethal. In the present study, we have generated L6 rat skeletal muscle cell-based model system for GNE Myopathy where GNE gene is knocked out at exon 3 using AAV mediated SEPT homology recombination. The cell line was heterozygous for GNE gene with one wild type and one truncated allele as confirmed by sequencing. The phenotype showed reduced GNE epimerase activity with little reduction in sialic acid content. In addition, the GNE knock out cell line revealed altered cytoskeletal organization with disrupted actin filament. The signaling cascade regulating actin dynamics such as Rho A, Cofilin, FAK and Src were altered leading to reduced cell migration in GNE heterozygous cells. Our study indicates possible role of GNE in regulating actin dynamics and cell migration of skeletal muscle cell. The skeletal muscle cell based system offers great potential in understanding pathomechanism and target identification for GNE Myopathy.
New Methods for Stress Concentration Factor Calculation in Butt Welded Joints: A Comparative Study
Authors: Randic Miroslav , Pavletić Duško , Fabić Marko
Published: June 2020

Surface cracks in butt-welded joints usually occur in places with increased stress concentrations. The stress concentration factor (SCF) can be calculated using an empirical equation, with five geometric parameters of a butt-welded joint (thickness of the base material, toe radius, weld toe angle, weld width, and reinforcement height). However, in anindustrial environment, it is impractical and sometimes even impossible to measure all five geometric parameters with sufficient accuracy. In this study, eight experiments on butt-welded joints were performed. All samples were scanned with a 3D scanner, and the geometric sizes of the welded joints were measured using computer software. A modified empirical expression proposed by Ushirokawa and Nakayama was used to calculate the SCF; the expression was adjusted in such a way that the SCF was calculated by knowing only the toe radius. In addition, four new expressions were proposed for the calculation of the SCF by knowing the toe radius in relation to the weld toe angle; the expressions were then compared and analysed. Additionally, the values of the stress concentrations in the butt-welded joints were obtained using afinite element method (FEM). The SCFs calculated using the four methods were compared and further discussed. Our data suggested a new accurate and straightforward approach for calculating the SCF by knowing only the weld toe radius.
Lifestyle-associated health risk indicators across a wide range of occupational groups: a cross-sectional analysis in 72 855 workers
Authors: Väisänen Daniel , Kallings Lena V , Andersson Gunnar , Wallin Peter , Hemmingsson Erik , Ekblom-Bak Elin
Published: June 2020

ObjectivesIdentify and compare health risk indicators for common chronic diseases between different occupational groups. Methods A total of 72,855 participants (41% women) participating in an occupational health servicescreening in 2014–2019 were included. Occupation was defined by the Swedish Standard Classification of Occupation, and divided into nine major and additionally eight sub-major groups. These were analysed separately, as white- and blue-collar occupations and as low- and high-skilled occupations. Seven health risk indicators were self-reported: exercise, physical work situation, sitting at work and leisure, smoking, diet, and perceived health, whereas cardiorespiratory fitness, BMI and blood pressure were measured. These were further dichotomized (yes/no) and as clustering of risk indicators (≥3 vs. <3).ResultsThe greatest variation in OR across sub-major and major occupational groups were seen for daily smoking (OR=0.68 to OR=5.12), physically demanding work (OR=0.55 to OR=45.74) and high sitting at work (OR=0.04 to OR=1.86). For clustering of health risk indicators, blue-collar workers had significantly higher clustering of health risks (OR: 1.80; 95% CI 1.71-1.90) compared to white-collar workers (reference). Compared to high-skilled white-collar workers, low-skilled white-collar workers had similar OR (2.00; 1.88-2.13) as high-skilled blue-collar workers (1.98; 1.86-2.12), with low-skilled blue-collar workers having the highest clustered risk (2.32; 2.17-2.48).ConclusionThere were large differences in health risk indicators across occupational groups, mainly between high-skilled white-collar occupations and the other occupations, with important variations also between major and sub-major occupational groups. Future health interventions should target the occupational groups identified with the highest risk for effective disease prevention.
Decreased MicroRNA-155 in Behcet’s disease leads to defective control of autophagy thereby stimulating excessive proinflammatory cytokine production
Authors: Liang Liang , Zhou Qingyun , Feng Lujia , Kijlstra Aize , Yang Peizeng
Published: June 2020

Background: Earlier we reported that the microRNA (miR)-155 expression in dendritic cells (DCs) from Behcet’s disease (BD) patients was decreased and affected cytokine production of DCs. In this study, we investigated the mechanisms whereby miR-155 regulates cytokine production by DCs.Methods: The formation of autophagosomes in DCs was detected by transmission electron microscopy. Western blotting was used to detect the protein levels of LC3, Beclin-1, P62, p-mTOR and p-Akt in DCs. TNF-α, IL-6 and IL-1β expression were investigated by ELISA. MiR-155 mimics were transfected to DCs to evaluate its effects on autophagy and cytokine production. RNA interference was used to downregulate the expression of TAB2.Results: The formation of autophagosomes was found in DCs of active BD patients. The expressions of LC3-Ⅱ, Beclin-1, P62, IL-6, IL-1β and TNF-α were significantly increased in DCs of active BD patients compared to that of healthy controls. The autophagy promoter (3-MA) and inhibitor (rapamycin) significantly decreased or increased the expression of TNF-α, IL-6 and IL-1β by DCs. The expression of LC3-Ⅱand Beclin-1 were significantly increased, but the expression of P62 proteins was decreased in DCs transfected with miR-155 mimics or after TAB2 was downregulated. The expression of TNF-α, IL-6 and IL-1β were decreased in DCs after miR-155 was upregulated or TAB2 was downregulated. The ratios of p-Akt/Akt and p-mTOR/mTOR were decreased in DCs after miR-155 was upregulated.Conclusions: These results suggest that miR-155 affects the production of TNF-α, IL-6 and IL-1β by DCs through activation of the Akt/mTOR signaling pathway and by affecting the process of autophagy.
Early Detection Of Deep Vein Thrombosis In Patients With Coronavirus Disease 2019: Who to Screen and Who not to with Doppler Ultrasound?
Authors: Ierardi Anna Maria , Coppola Andrea , Fusco Stefano , Stellato Elvira , Aliberti Stefano , Andrisani Maria Carmela , Vespro Valentina , Arrichiello Antonio , Panigada Mauro , Monzani Valter , Grasselli Giacomo , Venturini Massimo , Rehani Bhavya , Peyvandi Flora , Pesenti Antonio , Blasi Francesco , Carrafiello Gianpaolo
Published: June 2020

BACKGROUND. Aim of the study is to evaluate the incidence of DVT in COVID-19 patients and its correlation with the severity of the disease and with clinical and laboratory findings.METHODS. 234 symptomatic patients with COVID-19, diagnosed according to the World Health Organization guidelines, were included in the study. The severity of the disease was classified as moderate, severe and critical. Doppler ultrasound (DUS) was performed in all patients. DUS findings, clinical, laboratory’s and therapeutic variables were investigated by contingency tables, Pearson chi square test and by Student T test and Fisher's exact test. ROC curve analysis was applied to study significant continuous variables.RESULTS. Overall incidence of DVT was 10.7% (25/234): 1.6% (1/60) among moderate cases, 13.8% (24/174) in severely and critically ill patients. Prolonged bedrest and intensive care unit admission were significantly associated with the presence of DVT (19.7%). Fraction of inspired oxygen, P/F ratio, respiratory rate, heparin administration, D-dimer, IL-6, ferritin and CRP showed correlation with DVT. CONCLUSIONS. DUS may be considered a useful and valid tool for early identification of DVT. In less severely affected patients, DUS as screening of DVT might be unnecessary. High rate of DVT found in severe patients and its correlation with respiratory parameters and some significant laboratory findings suggests that these can be used as a screening tool for patients who should be getting DUS.
Effect of Untreated Oil Palm Fruit Fibre on the Engineering Properties of Road Construction Earth Materials
Authors: Nwakaire Chidozie Maduabuchukwu , Evaristus Ukomadu Chidebele , Elijah Onu Chijioke
Published: June 2020

This study first evaluated the engineering properties of lateritic soil samples from three selected locations in South Eastern Nigeria (Nawfia, Okpuno, and Ugwuoba). The effect of Oil Palm Fruit Fibre (OPFF) on the properties of these soil materials was investigated, with special emphasis on road construction applicability. The samples were subjected to different tests to ascertain their index properties. Varying proportions of OPFF from 0.5% to 3% were incorporated into the soil samples and the effects were observed based on compaction and California Bearing Ratio (CBR) results. The control samples without inclusion of OPFF achieved the highest Maximum Dry Densities (MDD), the MDDs reduced linearly as the OPFF content increased. Consequently, the CBR values decreased with increase in OPFF. The reduction in MDD for the Nawfia samples ranged from 0.29% to 5.31% as the OPFF increased from 0.5% to 3%. The MDD of the Okpuno sample also reduced within the range of 1.25% to 9.51% whereas the reduction for the Ugwuoba soil is within the range of 1.55% and 9.79%. With the inclusion of the OPFF, there was up to 75% to 83% drop in CBR values of the soil rendering them very unsuitable for pavement subgrade. It was concluded that the presence of fibre depreciated the engineering properties of the earth materials. Direct application of OPFF in any part of road pavement has been dissuaded.