bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial and Cell-Friendly Properties of Cobalt and Nickel-Doped Tricalcium Phosphate Ceramics
Authors: Deyneko Dina V. , Lebedev Vladimir N. , Barbaro Katia , Titkov Vladimir V. , Lazoryak Bogdan I. , Fadeeva Inna V. , Gosteva Alevtina N. , Udyanskaya Irina L. , Aksenov Sergey M. , Rau Julietta V.
Published: December 2023

β-Tricalcium phosphate (β-TCP) is widely used as bone implant material. It has been observed that doping the β-TCP structure with certain cations can help in combating bacteria and pathogenic microorganisms. Previous literature investigations have focused on tricalcium phosphate structures with silver, copper, zinc, and iron cations. However, there are limited studies available on the biological properties of β-TCP containing nickel and cobalt ions. In this work, Ca10.5−xNix(PO4)7 and Ca10.5−xCox(PO4)7 solid solutions with the β-Ca3(PO4)2 structure were synthesized by a high-temperature solid-state reaction. Structural studies revealed the β-TCP structure becomes saturated at 9.5 mol/% for Co2+ or Ni2+ ions. Beyond this saturation point, Ni2+ and Co2+ ions form impurity phases after complete occupying of the octahedral M5 site. The incorporation of these ions into the β-TCP crystal structure delays the phase transition to the α-TCP phase and stabilizes the structure as the temperature increases. Biocompatibility tests conducted on adipose tissue-derived mesenchymal stem cells (aMSC) using the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay showed that all prepared samples did not exhibit cytotoxic effects. Furthermore, there was no inhibition of cell differentiation into the osteogenic lineage. Antibacterial properties were studied on the C. albicans fungus and on E. coli, E. faecalis, S. aureus, and P. aeruginosa bacteria strains. The Ni- and Co-doped β-TCP series exhibited varying degrees of bacterial growth inhibition depending on the doping ion concentration and the specific bacteria strain or fungus. The combination of antibacterial activity and cell-friendly properties makes these phosphates promising candidates for anti-infection bone substitute materials.
Advancing Sensitivity in Guided-Wave Surface Plasmon Resonance Sensor through Integration of 2D BlueP/MoS2 Hybrid Layers
Authors: Yuan Xixi , Wu Leiming , Qin Yuwen
Published: December 2023

The surface plasmon resonance (SPR) signal, generated from the Kretschmann configuration, has been developed as an effective detection technology in chemical and biological sensors. The sensitivity of SPR signals to changes in the surrounding media makes it a valuable tool, as even a slight variation in refractive index can cause a significant change in SPR signals, such as phase, intensity, and resonance angle. However, the detection of ultralow changes in refractive index, which occur in chemical reactions or biological actions, remains a challenge for conventional SPR sensors due to their limited sensitivity. To overcome this limitation, we theoretically propose a novel guided-wave SPR (GWSPR) configuration coated with a few-layer blue phosphorene (blueP)/MoS2 hybrid structure. This configuration aims to enhance the electric field and subsequently achieve a significant improvement in sensitivity. The results of our study demonstrate that the proposed blueP/MoS2-based GWSPR sensor exhibits a high sensitivity of 290°/RIU, which represents an impressive enhancement of approximately 82.4% compared to the conventional Au-based SPR sensor. This advancement addresses the challenge of detecting ultralow changes in refractive index and offers significant potential for enhancing the performance of chemical and biological sensors.
Event-Related Brain Potentials N140 and P300 during Somatosensory Go/NoGo Tasks Are Modulated by Movement Preparation
Authors: Matsuda Yuya , Sugawara Yasushi , Akaiwa Mayu , Saito Hidekazu , Shibata Eriko , Sasaki Takeshi , Sugawara Kazuhiro
Published: December 2023

The Go/NoGo task requires attention and sensory processing to distinguish a motor action cue or ‘Go stimulus’ from a ‘NoGo stimulus’ requiring no action, as well as motor preparation for a rapid Go stimulus response. The neural activity mediating these response phases can be examined non-invasively by measuring specific event-related brain potentials (ERPs) using electroencephalography. However, it is critical to determine how different task conditions, such as the relationship between attention site and movement site, influence ERPs and task performance. In this study, we compared attention-associated ERP components N140 and P300, the performance metrics reaction time (RT) and accuracy (%Error) and movement-related cortical potentials (MRCPs) between Go/NoGo task trials in which attention target and movement site were the same (right index finger movement in response to right index finger stimulation) or different (right index finger movement in response to fifth finger stimulation). In other Count trials, participants kept a running count of target stimuli presented but did not initiate a motor response. The N140 amplitudes at electrode site Cz were significantly larger in Movement trials than in Count trials regardless of the stimulation site–movement site condition. In contrast, the P300 amplitude at Cz was significantly smaller in Movement trials than in Count trials. The temporal windows of N140 and P300 overlapped with the MRCP. This superposition may influence N140 and P300 through summation, possibly independent of changes in attentional allocation.
Thirty Risk Factors for Alzheimer’s Disease Unified by a Common Neuroimmune–Neuroinflammation Mechanism
Authors: Weaver Donald F.
Published: December 2023

One of the major obstacles confronting the formulation of a mechanistic understanding for Alzheimer’s disease (AD) is its immense complexity—a complexity that traverses the full structural and phenomenological spectrum, including molecular, macromolecular, cellular, neurological and behavioural processes. This complexity is reflected by the equally complex diversity of risk factors associated with AD. However, more than merely mirroring disease complexity, risk factors also provide fundamental insights into the aetiology and pathogenesis of AD as a neurodegenerative disorder since they are central to disease initiation and subsequent propagation. Based on a systematic literature assessment, this review identified 30 risk factors for AD and then extended the analysis to further identify neuroinflammation as a unifying mechanism present in all 30 risk factors. Although other mechanisms (e.g., vasculopathy, proteopathy) were present in multiple risk factors, dysfunction of the neuroimmune–neuroinflammation axis was uniquely central to all 30 identified risk factors. Though the nature of the neuroinflammatory involvement varied, the activation of microglia and the release of pro-inflammatory cytokines were a common pathway shared by all risk factors. This observation provides further evidence for the importance of immunopathic mechanisms in the aetiopathogenesis of AD.
Shifting Perceptions and Emotional Responses to Autonomous Vehicles Using Simulated Experiences
Authors: Tapia Jose L. , Sánchez-Borda David , Iniesta Carmen , Badea Francisco , Duñabeitia Jon Andoni
Published: December 2023

The societal integration of autonomous vehicles (AVs) relies on public acceptance, closely related to individual emotions and perceptions. This study explores the emotional factors affecting AV acceptance in Spain through lexical tasks, virtual AV simulations, and questionnaires, surpassing traditional attitude surveys by examining subtle emotional and lexical reactions to AVs. Acceptance was measured in terms of AV knowledge, perception of autonomous driving, and safety, with emphasis on lexical-emotional analysis after simulation. Findings indicate gender differences in AV acceptance, with women showing less knowledge and comfort with AV technology. Simulation improved understanding and generated more positive responses. This study shows how lexical tasks can reveal emotional influences on AV perception and suggests a wider approach to assess technology acceptance. These findings aid in creating campaigns and experiences to enhance public AV acceptance, mindful of demographic differences. Future studies should extend this framework to various populations to investigate the emotional lexicon’s role in AV acceptance.
Determining Urban Indicators in Local Plans—As One of the Sustainable Assumptions of the New European Bauhaus?
Authors: Andrzejewska Anna Katarzyna
Published: December 2023

The purpose of local spatial development plans in Poland is to shape spatial policy at the local level by establishing local law. On this basis, the intended use of land is determined, as well as the manner of its development. Some of the planning tools used are urban planning parameters and indicators, which are defined in planning documents at the local (commune) level. This article discusses the analyses of two selected urban indicators: the maximum built-up area and the minimum share of biologically active area, which are obligatorily determined in local plans. The issue here is a certain discrepancy between the planning provisions used in theory and their practical application. This paper uses a descriptive, computational, and comparative analytical research method to interpret the planning provisions used in selected Local Spatial Development Plans. This research will allow for answering of the following questions: Are the urban indicators defined by architects adapted to spatial needs? Do they allow for optimal shaping of this space, taking into account the provisions of Polish law? And is their skillful designation likely to be one of the sustainable assumptions of the New European Bauhaus?
High-Density Communities and Infectious Disease Vulnerability: A Built Environment Perspective for Sustainable Health Development
Authors: Hu Yue , Lin Ziyi , Jiao Sheng , Zhang Rongpeng
Published: December 2023

High-density communities have proliferated globally during rapid urbanization. They are characterized by a high population density and limited per capita public spaces, making them susceptible to infectious disease risks. The impact of infectious diseases in these communities, as evident during the COVID-19 pandemic, underscores their vulnerabilities. Yet, research on disease prevention in high-density areas remains limited. This study aims to investigate the relationship between the built environment and the transmission of infectious diseases in high-density urban communities, with a particular focus on the lessons learned during the COVID-19 pandemic. Utilizing Shenzhen city as a case study, this study collected data on the built environment and epidemic trends and involved a generalized linear regression analysis, aiming to understand the key built environment factors that affect epidemic spread in high-density areas. The results from the study revealed that high-density communities experience higher rates of infectious disease transmission compared to their medium- to low-density counterparts. The significant factors identified include land use mixture and walkability, with land use mixture showing the most substantial impact on infection rates. Through a combination of qualitative analysis and empirical research, we constructed a conceptual framework linking containment measures, non-pharmaceutical interventions, and the built environment. The findings emphasize the significance to focus on the health development of high-density communities and offer valuable insights for tailored urban planning and built environment design. These insights are crucial for promoting the healthy and sustainable transformation of existing high-density communities.
Aeolian Vibration Dynamic Analysis of Large-Span, Relaxed Antenna Cable Net Based on Finite Particle Method
Authors: Qin Kai , Zhao Fan , Luo Yaozhi , Fang Bin , Chen Shangyuan
Published: December 2023

The large-span, relaxed antenna network is a large deformation flexible structure due to its low pre-tension level of the wires. Its dynamic analysis under a wind load belongs to dynamic and geometric nonlinear problems, which is very complex to accurately calculate and solve. This paper explores the possibility of the finite particle method (FPM) to the aeolian vibration analysis of a large-span, low stress-tensioned antenna cable net. In the FPM, the antenna network structure is discretized into a group of finite particles, where the motions of all particles follow Newton’s second law and can be solved dynamically using a central difference scheme. The effectiveness and applicability of the FPM were verified by comparing the calculation results of the finite element method and FPM. The FPM was used to study the effects of wind speed and the distribution of vibration on the aeolian vibration of antenna cable nets. The results showed that this method is suitable for studying the aeolian vibration of a large-span, low stress-tensioned antenna network and has high computational efficiency and accuracy.
Typical Village and Town Houses in the Cold Region of Northeast China: Simulation Analysis of Courtyard Layout and Winter Wind Environment Habitability
Authors: Sun Ruiheng , Dong Junyan , Zhao Hui , Wang Liying , Qin Di
Published: December 2023

Folk residence in the cold region of Northeast China is an integral part of China’s traditional folk residence, with significant geographical representation. In this paper, we take the residential compounds of villages and towns in the cold region of Northeast China as the research object, summarize four typical layout forms of residential compounds of villages and towns in the cold region of Northeast China, simulate the wind environment of combinations of different layout forms in winter using the computer numerical simulation software Fluent, and then analyze and evaluate the wind environment of the compounds, including the evaluation standard of the wind speed ratio and considering the area of wind shadows, number of vortices, and other factors.
Discrepancy between Tumor Size Assessed by Full-Field Digital Mammography or Ultrasonography (cT) and Pathology (pT) in a Multicenter Series of Breast Metaplastic Carcinoma Patients
Authors: Püsküllüoğlu Mirosława , Świderska Katarzyna , Konieczna Aleksandra , Rudnicki Wojciech , Pacholczak-Madej Renata , Kunkiel Michał , Grela-Wojewoda Aleksandra , Mucha-Małecka Anna , Mituś Jerzy W. , Stobiecka Ewa , Ryś Janusz , Jarząb Michał , Ziobro Marek
Published: December 2023

Metaplastic breast cancer (BC-Mp) presents diagnostic and therapeutic complexities, with scant literature available. Correct assessment of tumor size by ultrasound (US) and full-field digital mammography (FFDM) is crucial for treatment planning. Methods: A retrospective cohort study was conducted on databases encompassing records of BC patients (2012–2022) at the National Research Institutes of Oncology (Warsaw, Gliwice and Krakow Branches). Inclusion criteria comprised confirmed diagnosis in postsurgical pathology reports with tumor size details (pT) and availability of tumor size from preoperative US and/or FFDM. Patients subjected to neoadjuvant systemic treatment were excluded. Demographics and clinicopathological data were gathered. Results: Forty-five females were included. A total of 86.7% were triple-negative. The median age was 66 years (range: 33–89). The median pT was 41.63 mm (6–130), and eight patients were N-positive. Median tumor size assessed by US and FFDM was 31.81 mm (9–100) and 34.14 mm (0–120), respectively. Neither technique demonstrated superiority (p > 0.05), but they both underestimated the tumor size (p = 0.002 for US and p = 0.018 for FFDM). Smaller tumors (pT1-2) were statistically more accurately assessed by any technique (p < 0.001). Only pT correlated with overall survival. Conclusion: The risk of underestimation in tumor size assessment with US and FFDM has to be taken into consideration while planning surgical procedures for BC-Mp.
Dysregulation of Peripheral Blood Mononuclear Cells and Immune-Related Proteins during the Early Post-Operative Immune Response in Ovarian Cancer Patients
Authors: Ulevicius Jonas , Jasukaitiene Aldona , Bartkeviciene Arenida , Dambrauskas Zilvinas , Gulbinas Antanas , Urboniene Daiva , Paskauskas Saulius
Published: December 2023

Surgical treatment is a cornerstone of ovarian cancer (OC) therapy and exerts a substantial influence on the immune system. Immune responses also play a pivotal and intricate role in OC progression. The aim of this study was to investigate the dynamics of immune-related protein expression and the activity of peripheral blood mononuclear cells (PBMCs) in OC patients, both before surgery and during the early postoperative phase. The study cohort comprised 23 OC patients and 20 non-cancer controls. A comprehensive analysis of PBMCs revealed significant pre-operative downregulation in the mRNA expression of multiple immune-related proteins, including interleukins, PD-1, PD-L1, and HO-1. This was followed by further dysregulation during the first 5 post-operative days. Although most serum interleukin concentrations showed only minor changes, a distinct increase in IL-6 and HO-1 levels was observed post-operatively. Reduced metabolic and phagocytic activity and increased production of reactive oxygen species (ROS) were observed on day 1 post-surgery. These findings suggest a shift towards immune tolerance during the early post-operative phase of OC, potentially creating a window for treatment. Further research into post-operative PBMC activity could lead to the development of new or improved treatment strategies for OC.
Limited Effects of Class II Transactivator-Based Immunotherapy in Murine and Human Glioblastoma
Authors: Tan A. Katherine , Henry Aurelie , Goffart Nicolas , van Logtestijn Sofie , Bours Vincent , Hol Elly M. , Robe Pierre A.
Published: December 2023

Background: The major histocompatibility complex type II is downregulated in glioblastoma (GB) due to the silencing of the major transcriptional regulator class II transactivator (CIITA). We investigated the pro-immunogenic potential of CIITA overexpression in mouse and human GB. Methods: The intracerebral growth of wildtype GL261-WT cells was assessed following contralateral injection of GL261-CIITA cells or flank injections with GL261-WT or GL261-CIITA cells. Splenocytes obtained from mice implanted intracerebrally with GL261-WT, GL261-CIITA cells or phosphate buffered saline (PBS) were transferred to other mice and subsequently implanted intracerebrally with GL261-WT. Human GB cells and (syngeneic) GB-infiltrating immune cells were isolated from surgical samples and co-cultured with GB cells expressing CIITA or not, followed by RT-qPCR assessment of the expression of key immune regulators. Results: Intracerebral vaccination of GL261-CIITA significantly reduced the subsequent growth of GL261-WT cells implanted contralaterally. Vaccination with GL261-WT or -CIITA subcutaneously, however, equivalently retarded the intracerebral growth of GL261 cells. Adoptive cell transfer experiments showed a similar antitumor potential of lymphocytes harvested from mice implanted intracerebrally with GL261-WT or -CIITA. Human GB-infiltrating myeloid cells and lymphocytes were not activated when cultured with CIITA-expressing GB cells. Tumor-infiltrating NK cells remained mostly inactivated when in co-culture with GB cells, regardless of CIITA. Conclusion: these results question the therapeutic potential of CIITA-mediated immunotherapy in glioblastoma.
18F-Fluoroethyl-L Tyrosine Positron Emission Tomography Radiomics in the Differentiation of Treatment-Related Changes from Disease Progression in Patients with Glioblastoma
Authors: Manzarbeitia-Arroba Begoña , Hodolic Marina , Pichler Robert , Osipova Olga , Soriano-Castrejón Ángel Maria , García-Vicente Ana María
Published: December 2023

The follow-up of glioma patients after therapeutic intervention remains a challenging topic, as therapy-related changes can emulate true progression in contrast-enhanced magnetic resonance imaging. 18F-fluoroethyl-tyrosine (18F-FET) is a radiopharmaceutical that accumulates in glioma cells due to an increased expression of L-amino acid transporters and, contrary to gadolinium, does not depend on blood–brain barrier disruption to reach tumoral cells. It has demonstrated a high diagnostic value in the differentiation of tumoral viability and pseudoprogression or any other therapy-related changes, especially when combining traditional visual analysis with modern radiomics. In this review, we aim to cover the potential role of 18F-FET positron emission tomography in everyday clinical practice when applied to the follow-up of patients after the first therapeutical intervention, early response evaluation, and the differential diagnosis between therapy-related changes and progression.
Immunomodulatory Effects of Primed Tonsil-Derived Mesenchymal Stem Cells on Atopic Dermatitis via B Cell Regulation
Authors: Kim Dong-Kyu , Lee Hyun-Joo , Lee Il Hwan , Lee Jae-Jun
Published: December 2023

Mesenchymal stem cells (MSCs) ameliorate T-and B cell-mediated immune responses. In particular, tonsil-MSCs (T-MSCs) are attractive candidates for practical and clinical applications because of their ease of acquisition and relatively low immunogenicity compared with other MSC sources. The use of MSCs as a therapeutic tool in atopic dermatitis (AD) has been investigated, but that of T-MSCs remains to be explored. Therefore, we investigated the immunomodulatory effects of primed T-MSCs in AD pathogenesis. In our animal study, primed T-MSCs showed greater immunological suppressive effects than naïve T-MSCs. Additionally, in vitro, the proliferation of B cells was downregulated by the addition of primed T-MSCs compared with naïve T-MSCs. The activation of B cells to differentiate into antibody-secreting cells and produce IgE was also reduced when primed T-MSCs were added. Moreover, under CD40-knockdown conditions, we found that CD40 in primed T-MSCs played a critical role as a regulator of B cell activation and was mediated by the non-canonical NF-κB pathway. Therefore, our findings suggest a promising role for primed T-MSCs in the treatment of AD by regulating B cell-mediated inflammatory responses, which are dependent on CD40 expression on primed T-MSCs mediated through the non-canonical NF-κB pathway.
Is Environmental Cadmium Exposure Causally Related to Diabetes and Obesity?
Authors: Satarug Soisungwan
Published: December 2023

Cadmium (Cd) is a pervasive toxic metal, present in most food types, cigarette smoke, and air. Most cells in the body will assimilate Cd, as its charge and ionic radius are similar to the essential metals, iron, zinc, and calcium (Fe, Zn, and Ca). Cd preferentially accumulates in the proximal tubular epithelium of the kidney, and is excreted in urine when these cells die. Thus, excretion of Cd reflects renal accumulation (body burden) and the current toxicity of Cd. The kidney is the only organ other than liver that produces and releases glucose into the circulation. Also, the kidney is responsible for filtration and the re-absorption of glucose. Cd is the least recognized diabetogenic substance although research performed in the 1980s demonstrated the diabetogenic effects of chronic oral Cd administration in neonatal rats. Approximately 10% of the global population are now living with diabetes and over 80% of these are overweight or obese. This association has fueled an intense search for any exogenous chemicals and lifestyle factors that could induce excessive weight gain. However, whilst epidemiological studies have clearly linked diabetes to Cd exposure, this appears to be independent of adiposity. This review highlights Cd exposure sources and levels associated with diabetes type 2 and the mechanisms by which Cd disrupts glucose metabolism. Special emphasis is on roles of the liver and kidney, and cellular stress responses and defenses, involving heme oxygenase-1 and -2 (HO-1 and HO-2). From heme degradation, both HO-1 and HO-2 release Fe, carbon monoxide, and a precursor substrate for producing a potent antioxidant, bilirubin. HO-2 appears to have also anti-diabetic and anti-obese actions. In old age, HO-2 deficient mice display a symptomatic spectrum of human diabetes, including hyperglycemia, insulin resistance, increased fat deposition, and hypertension.
Evaluation of the Essential Oil Composition of Five Thymus Species Native to Greece
Authors: Kakouri Eleni , Daferera Dimitra , Andriopoulou Anastasia , Trigas Panayiotis , Tarantilis Petros A.
Published: December 2023

The genus Thymus encompasses a wide array of taxa, many of which remain underexplored in terms of their phytochemical profile. In this study, we investigated the phytochemical composition of volatile compounds of five Thymus species native to Greece using gas chromatography combined with mass spectrometry. Two samples of T. parnassicus collected from Mts Parnitha and Parnassos were studied. The predominant compounds in the Parnitha sample were α-cadinol (13.53%), E-caryophyllene (11.83%) and selin-11-en-4α-ol (7.29%). The sample from Mt. Parnassos exhibited a high concentration of E-caryophyllene (35.20%) followed by β-bisabolene (10.41%). Additionally, two species, namely T. leucotrichus subsp. leucotrichus and T. atticus, were collected on Mt. Chelmos (Peloponnese). The essential oil of T. leucotrichus was rich in elemol (35.56%), α-eudesmol (11.15%) and β-eudesmol (6.11%). Thymus atticus exhibited a high concentration in linalool (63.04%) and p-cymene (25.63%). In addition, two samples of T. holosericeus collected from Kefalonia Ιsland were both rich in geraniol (89.9% and 87.7%, respectively). We also examined the volatile profile of T. laconicus, a local endemic species of SE Peloponnese (Lakonia area), which remains unexplored. Carvacrol (32.7%) and p-cymene (29.7%) were identified as the dominant compounds. Our study contributes valuable insights into the chemical profile of Thymus spp. and sheds further light on the well-known chemical polymorphism within this genus.
Diagnostic Utility of Interleukin-6 in Early-Onset Sepsis among Term Newborns: Impact of Maternal Risk Factors and CRP Evaluation
Authors: Schleier Maria , Lubig Julia , Kehl Sven , Hébert Steven , Woelfle Joachim , van der Donk Adriana , Bär Alisa , Reutter Heiko , Hepp Tobias , Morhart Patrick
Published: December 2023

(1) Background: Interleukin-6 (IL-6) levels act as an early infection marker preceding C-reactive protein (CRP) elevation. This study seeks to analyze IL-6 behavior in suspected early-onset sepsis (EOS) cases among term newborns, comparing it to that of CRP and evaluating IL-6’s diagnostic utility. We also aim to assess the impact of maternal risk factors on EOS in term newborns, quantifying their influence for informed decision making. (2) Methods: The retrospective data analysis included 533 term newborns who were admitted to our hospital because of suspected EOS. IL-6, CRP, and the impact of maternal risk factors were analyzed in the context of EOS using binomial test, Chi-squared test, logistic and linear regression. (3) Results: In the cases of EOS, both IL-6 and CRP were elevated. The increase in CRP can be predicted by the initial increase in IL-6 levels. Among the assessed risk factors, intrapartum maternal fever (adjusted odds ratio 18.1; 95% CI (1.7–4.1)) was identified as the only risk factor significantly associated with EOS. (4) Conclusions: Employing IL-6 as an early infection marker enhanced EOS diagnostic precision due to its detectable early rise. However, caution is required, as elevations in IL-6 and CRP levels do not exclusively indicate EOS. Increased CRP levels in healthy newborns with maternal risk factors may be attributed to dynamics of vaginal labor.
Indocyanine Green (ICG) Fluorescence-Assisted Open Surgery Using the Rubina® Lens System in the Pediatric Population: A Single-Center Prospective Case Series
Authors: Esposito Ciro , Di Mento Claudia , Chiodi Annalisa , Cerulo Mariapina , Coppola Vincenzo , Del Conte Fulvia , Carraturo Francesca , Esposito Giovanni , Escolino Maria
Published: December 2023

Introduction: There are scarce papers about the use of fluorescence-guided surgery (FGS) in the open surgical field. This study aimed to assess the usefulness of FGS in an open setting in the pediatric population and to report our preliminary experience using the Rubina® Lens system. Methods: All patients undergoing ICG fluorescence-assisted open surgery over the period September 2022–September 2023 were enrolled. Each surgical procedure was performed using the Rubina® Lens for ICG fluorescence visualization. Results: A total of 25 patients, 14 boys and 11 girls with a median age at surgery of 5.8 years-old (range 0–15), were enrolled. Surgical indications were dermoid/epidermoid cysts of the head (n = 7), lymphangiomas of the head/neck (n = 2), thyroglossal duct cysts (n = 7), gynecomastia (n = 3), preauricular fistula (n = 2), second branchial cleft fistula (n = 1), fibrolipoma of the shoulder (n = 1) and myofibroma of the gluteal/perineal region (n = 2). In all procedures, an intralesional injection of 2.5 mg/mL ICG solution using a 30-gauge needle was administered. No adverse reactions to ICG occurred. Median operative time was 68.6 min (range 35–189). The visualization of ICG-NIRF with the Rubina® Lens was achieved in all cases. No intraoperative complications were reported. Postoperative complications occurred in 3/25 patients (12%), with gynecomastia (n = 1), thyroglossal duct cyst (n = 1) and neck lymphangioma (n = 1), who developed a fluid collection in the surgical site, requiring needle aspiration in outpatient care (Clavien–Dindo 2). Complete mass excision was confirmed with pathology reports. Conclusions: Based on this initial experience, FGS using the Rubina® Lens was very helpful in open surgery, providing enhanced visualization of anatomy and identification of margins, real-time reliability and low complication rate. It was easy to use, time saving, feasible and clinically safe. Previous experience in MIS is necessary to adopt this technology. The accuracy of the injection phase is important to avoid diffusion of the ICG into the perilesional tissue.
Predictors of Urinary Abnormalities in Children Hospitalised for Their First Urinary Tract Infection
Authors: Buonsenso Danilo , Sodero Giorgio , Camporesi Anna , Pierucci Ugo Maria , Raffaelli Francesca , Proli Francesco , Valentini Piero , Rendeli Claudia
Published: December 2023

We aimed to investigate if children with their first UTI and a concomitant positive blood culture have a higher risk of abnormalities. We performed a retrospective study of children younger than 18 years of age with their first UTI. Multivariate logistic regression and receiver operating characteristic (ROC) curves were used to evaluate if positive blood cultures are associated with urinary abnormalities. After the screening process, we considered the enrolled 161 children with UTIs. The median age was three months, and 83 were females (43.2%). In multivariate analysis, age (p = 0.001, 95% CI 1.005–1.020), the presence of Pseudomonas aeruginosa or unusual germs in urine cultures (p = 0.002, 95% CI 2.18–30.36) and the positivity of blood cultures (p = 0.001, 95% CI 2.23–18.98) were significantly associated with urinary abnormalities. A model based on these parameters has an AUC of 0.7168 to predict urinary malformations (p = 0.0315). Conclusions include how greater age, a positive blood culture and the presence of Pseudomonas aeruginosa or unusual germs in urine culture in children hospitalised for their first episode of a UTI are factors associated with a significantly higher risk of urinary abnormalities. These data can guide the implementation of more personalized strategies to screen for urinary abnormalities that may be included in future guidelines.
Sonothrombolysis: State-of-the-Art and Potential Applications in Children
Authors: Ward Rebecca E. , Martinez-Correa Santiago , Tierradentro-García Luis Octavio , Hwang Misun , Sehgal Chandra M.
Published: December 2023

In recent years, advances in ultrasound therapeutics have been implemented into treatment algorithms for the adult population; however, the use of therapeutic ultrasound in the pediatric population still needs to be further elucidated. In order to better characterize the utilization and practicality of sonothrombolysis in the juvenile population, the authors conducted a literature review of current pediatric research in therapeutic ultrasound. The PubMed database was used to search for all clinical and preclinical studies detailing the use and applications of sonothrombolysis, with a focus on the pediatric population. As illustrated by various review articles, case studies, and original research, sonothrombolysis demonstrates efficacy and safety in clot dissolution in vitro and in animal studies, particularly when combined with microbubbles, with potential applications in conditions such as deep venous thrombosis, peripheral vascular disease, ischemic stroke, myocardial infarction, and pulmonary embolism. Although there is limited literature on the use of therapeutic ultrasound in children, mainly due to the lower prevalence of thrombotic events, sonothrombolysis shows potential as a noninvasive thrombolytic treatment. However, more pediatric sonothrombolysis research needs to be conducted to quantify the safety and ethical considerations specific to this vulnerable population.
Dentist Mothers’ Attitudes, Challenges and Facilitators in the Oral Health Promotion of Their Young Children
Authors: Akşit-Bıçak Damla
Published: December 2023

Twice daily parental supervised brushing (PSB) is recommended for the effective oral hygiene of children with toothpaste including fluoride. This cross-sectional study aimed to identify dentist mothers’ attitudes, challenges, and facilitators in the oral health promotion of their young children. An online questionnaire was prepared and distributed to dentists via e-mail with a link to the form. Of all the mothers, 46.50% started brushing their children’s teeth after the eruption of the first primary teeth. Nearly 50% of the mothers thought that they should brush their children’s teeth until at least the age of 7–8 years old. In terms of the main barriers to toothbrushing, 52.9% said children could fall asleep at home or in the car, while 27.5% of all mothers reported being tired as the main barrier to toothbrushing. Although dentist mothers have sufficient knowledge to promote oral and dental health, they do not always have ideal attitudes and behaviors and they may encounter various barriers regarding themselves and their children in practical applications. Providing oral care for young children goes beyond ‘knowledge’ and is sometimes ‘challenging’; however, it could be ‘possible’ by developing and implementing the most appropriate solution strategies suitable for each unique family and child.
Comparison of Indirect and Direct Laryngoscopes in Pediatric Patients with a Difficult Airway: A Systematic Review and Meta-Analysis
Authors: Takeuchi Risa , Hoshijima Hiroshi , Mihara Takahiro , Kokubu Shinichi , Sato (Boku) Aiji , Nagumo Takumi , Mieda Tsutomu , Shiga Toshiya , Mizuta Kentaro
Published: December 2023

This meta-analysis was performed to determine whether an indirect laryngoscope is more advantageous than a direct laryngoscope for tracheal intubation in the setting of a difficult pediatric airway. Data on the intubation failure and intubation time during tracheal intubation were extracted from prospective and retrospective studies identified through a comprehensive literature search. Data from 10 individual articles (11 trials) were combined, and a DerSimonian and Laird random-effects model was used to calculate either the pooled relative risk (RR) or the weighted mean difference (WMD) and the corresponding 95% confidence interval (CI). Meta-analysis of the 10 articles indicated that the intubation failure of tracheal intubation with an indirect laryngoscope was not significantly different from that of a direct laryngoscope in patients with a difficult airway (RR 0.86, 95% CI 0.51–1.46; p = 0.59; Cochrane’s Q = 50.5; I2 = 82%). Intubation time with an indirect laryngoscope was also similar to that with a direct laryngoscope (WMD 4.06 s; 95% CI −1.18–9.30; p = 0.13; Cochrane’s Q 39.8; I2 = 85%). In conclusion, indirect laryngoscopes had the same intubation failure and intubation time as direct laryngoscopes in pediatric patients with a difficult airway. Currently, the benefits of indirect laryngoscopes have not been observed in the setting of a difficult pediatric airway.
Maternal Wellbeing Five Years after a Very Preterm Delivery: Prevalence and Influencing Factors in a European Cohort
Authors: Wohlers Lena , Maier Rolf F. , Cuttini Marina , Wilson Emilija , Benhammou Valérie , Lebeer Jo , Laroche Sabine , Sarrechia Iemke , Petrou Stavros , Thiele Nicole , Zeitlin Jennifer , Aubert Adrien M.
Published: December 2023

(1) Background: Mothers of very preterm (VPT) infants may experience psychological symptoms compromising long-term emotional wellbeing. This study describes the emotional wellbeing of mothers of five-year-old children born VPT. We assess the association between sociodemographic, perinatal and neonatal characteristics, and the child’s health and development at five years old and maternal emotional wellbeing. (2) Methods: Data are from the prospective European “Effective Perinatal Intensive Care in Europe” (EPICE) and subsequent “Screening for Health In very Preterm infantS in Europe” (SHIPS) projects including births <32 weeks’ gestational age in 11 countries in 2011/12. Data were abstracted from obstetric and neonatal records. At five years old, 2605 mothers answered a parental questionnaire including the Mental Health Inventory-5 (MHI-5). Associations between sociodemographic and health characteristics and the mother’s MHI-5 score were investigated using multilevel multivariate linear regression analysis with the country modelled as a random effect and inverse probability weighting to correct for attrition bias. (3) Results: The mean MHI-5 score was 71.3 (SD 16.7) out of 100 (highest emotional wellbeing) with a variation among countries from 63.5 (SD 16.8; Poland) to 82.3 (SD 15.8; the Netherlands). MHI-5 scores were significantly lower for mothers whose child had a severe health problem, developmental, or speech delay, for multiparous and single mothers, and when at least one of the parents was unemployed. (4) Conclusions: The emotional wellbeing of mothers of VPT infants differs between European countries. Identifying sociodemographic characteristics and child’s health and developmental conditions that affect maternal emotional wellbeing may help to identify groups of mothers who need special assistance to cope with consequences of the delivery of a VPT child.
Bitter Phytochemicals as Novel Candidates for Skin Disease Treatment
Authors: Grădinaru Teodora-Cristiana , Vlad Adelina , Gilca Marilena
Published: December 2023

Skin diseases represent a global healthcare challenge due to their rising incidence and substantial socio-economic burden. While biological, immunological, and targeted therapies have brought a revolution in improving quality of life and survival rates for certain dermatological conditions, there remains a stringent demand for new remedies. Nature has long served as an inspiration for drug development. Recent studies have identified bitter taste receptors (TAS2Rs) in both skin cell lines and human skin. Additionally, bitter natural compounds have shown promising benefits in addressing skin aging, wound healing, inflammatory skin conditions, and even skin cancer. Thus, TAS2Rs may represent a promising target in all these processes. In this review, we summarize evidence supporting the presence of TAS2Rs in the skin and emphasize their potential as drug targets for addressing skin aging, wound healing, inflammatory skin conditions, and skin carcinogenesis. To our knowledge, this is a pioneering work in connecting information on TAS2Rs expression in skin and skin cells with the impact of bitter phytochemicals on various beneficial effects related to skin disorders.
Individual Characteristics as Motivators of Sustainable Behavior in Electronic Vehicle Rental
Authors: Wang Yuchen , Gulzari Adeela , Prybutok Victor
Published: December 2023

This study investigates the understudied area of motivational factors influencing the rental intention of electric vehicles (EVs) within the context of their integration into urban transportation to combat air pollution and reduce carbon footprints and explores the critical factors influencing consumer behavior towards EV rental, focusing on hedonic motivation, service level, consumer habits, and willingness to pay. Utilizing multiple linear regression analysis on 302 valid samples from Texas, USA, the research identifies the significant impact of these factors on rental intention. Notably, the service level emerges as the most influential predictor while emphasizing the unique and less studied role of hedonic and personal characteristics as essential antecedents of rental intention. The findings, supplemented by a Monte Carlo simulation, reveal that these personal and motivational characteristics are pivotal in shaping rental intentions, accounting for approximately 47.2% of the variance in rental intention. The study contributes valuable insights into the EV rental market, offering theoretical implications for the EV literature and practical strategies for car rental enterprises to tap into consumer patterns effectively.
Assessment of Climate Risks, Vulnerability of Urban Health Systems, and Individual Adaptation Strategies in the City of N’Djaména (Chad)
Authors: Allarané Ndonaye , Atchadé Assouhan Jonas , Azagoun Vidjinnagni Vinasse Ametooyona , Houngnigbe Adanvo Isaac , Gouataine Seingue Romain , N’Dilbé Tob-Ro , Hetcheli Follygan
Published: December 2023

Climate variability and change are already having a negative impact on the health of tens of millions of Africans through exposure to sub-optimal temperatures and extreme weather conditions as well as increasing the range and transmission of infectious diseases. This study aims to identify climate risks and the vulnerability of health systems as well as individual coping strategies in the city of N’Djaména. To achieve this, we adopted a methodology combining both quantitative and qualitative approaches. Meteorological data on wind, temperature, and rainfall were collected at daily and monthly intervals from the National Meteorological Agency in N’Djaména. Qualitative data were collected via focus group discussions with targets of the city’s health system and quantitative data were collected from the population on the basis of oriented questionnaires. The results show that rising temperatures with heat waves, regular flooding, and strong winds are the major climate risks identified. These have numerous impacts and effects on the city’s health system due to the following vulnerability factors most recognized by city dwellers: insufficient medical equipment in health facilities (IEME), the fragile nature of people’s physiological state in the face of climatic risks (CFEP), and the failure of city sanitation strategies and policies (DSPA). This study proposes a set of recommendations for transformational adaptation of the healthcare sector, which remains vulnerable to climate risks.
A Study on the Structure, Optical Properties and Cellular Localization of Novel 1,3-Benzothiazole-Substituted BODIPYs
Authors: Kirkilessi Olga , Arapatzi Christina , Reis Heribert , Kostourou Vassiliki , Prousis Kyriakos C. , Calogeropoulou Theodora
Published: December 2023

A library of seven novel 1,3-benzothiazole-substituted BODIPY derivatives with tunable optical properties was synthesized. The new fluorescent dyes exhibited bathochromically shifted absorptions (up to 670 nm) and emissions centered in the red and near-infrared spectral region (up to 677 nm) in comparison to the parent compound 8-phenyl BODIPY (λabs: 499 nm, λemi: 508 nm). (TD)DFT calculations were performed to rationalize the spectroscopic properties of the new dyes. The cellular biodistribution of the new BODIPY dyes, their fluorescence stability and toxicity were investigated in both living and fixed fibroblasts using time-lapse fluorescent imaging and confocal microscopy. Six of the seven new dyes were photostable and non-toxic in vitro at 10 μM concentration. In addition, they efficiently stained the cell membrane, showing diffuse and dotty localization within the cell at low concentrations (1.0 and 0.1 μM). Specifically, dye TC498 was localized in vesicular structures in both live and fixed cells and could be used as a suitable marker in co-staining studies with other commonly used fluorescent probes.
High-Temperature Oxidation Behavior of FeCoCrNi+(Cu/Al)-Based High-Entropy Alloys in Humid Air
Authors: White Emma Marie Hamilton , Bürckner Mary-Lee , Schlereth Clara , Bik Maciej , Galetz Mathias Christian
Published: December 2023

Previous studies showed some transition metal high-entropy alloy (HEA) compositions can have good oxidation resistance in air up to 800 °C. Four equiatomic HEAs have been developed based on FeCoCrNi with additions of Mn, Cu, Al or Al+Cu. The oxidation behavior of these HEAs was compared in humid (10 vol.% H2O) air at 800 °C for 100–500 h to investigate the influence of water vapor on the oxidation mechanisms. The Cu- and Al-containing alloys exhibited improved oxidation resistance over the Mn composition. For the Cu-containing alloy, a local attack of the Cu-rich phase was observed, which formed an Fe/Ni/Co/Cr spinel that was surrounded by Cr2O3. This oxide was thicker for the humid air atmosphere when compared to dry air, and the transition of the Cu oxide to the spinel was accelerated. The Al-containing HEA formed a thin Al2O3 scale with humidity suppressing AlN formation and forming a smoother oxide layer. The Al+Cu composition had the highest overall oxidation resistance (minimal local attack, no nitridation) and also showed a smooth oxide scale topography under humid air oxidation as opposed to a plate-like, rougher scale under dry air.
The Two-Process Model: Origin of Its Concepts and Their Implications
Authors: Borbély Alexander , Tobler Irene
Published: December 2023

The two-process model of sleep regulation has served as a conceptual framework in the last four decades for understanding sleep physiology. In the 1970s, long-term recordings of sleep in rats were obtained thanks to EEG telemetry. NonREM sleep and REM sleep were found to differ in their time course and response to light-dark protocols. There were indications for their coupling to the circadian system, in particular the light-dark and the dark-light transitions. With the advent of quantitative EEG analysis, slow-wave activity in nonREM sleep was recognized as a sleep-wake-dependent variable. The term “sleep homeostasis” was coined to specify the regulated balance between sleep and waking. The regulatory homeostatic process was designated as “Process S”. In the two-process model, its interaction with the circadian pacemaker “Process C” can account for sleep duration under various experimental protocols. Local, use-dependent slow-wave activity changes were demonstrated in both humans and rats by the selective, unilateral activation of a cortical region prior to sleep. Finding that rest in invertebrates has sleep-like regulatory properties opened a new realm of animal studies. Comparative sleep studies in a broad variety of animal species confirmed the validity of the basic concepts of the two-process model. Recent studies have addressed sleep-related changes of brain temperature as an indicator of brain metabolism; the application of the model to Drosophila; the divergence of cortical and subcortical states; and sleep in an increasing number of species and taxa.