bluuebunny/crossref_metadata_2025 huggingface dataset:
Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease
Authors: D'Oliviera Angel , Dai Xuhang , Mottaghinia Saba , Olson Sophie , Geissler Evan P , Etienne Lucie , Zhang Yingkai , Mugridge Jeffrey S
Published: January 2025

The SARS-CoV-2 main protease (Mpro or Nsp5) is critical for production of viral proteins during infection and, like many viral proteases, also targets host proteins to subvert their cellular functions. Here, we show that the human tRNA methyltransferase TRMT1 is recognized and cleaved by SARS-CoV-2 Mpro. TRMT1 installs the N2,N2-dimethylguanosine (m2,2G) modification on mammalian tRNAs, which promotes cellular protein synthesis and redox homeostasis. We find that Mpro can cleave endogenous TRMT1 in human cell lysate, resulting in removal of the TRMT1 zinc finger domain. Evolutionary analysis shows the TRMT1 cleavage site is highly conserved in mammals, except in Muroidea, where TRMT1 is likely resistant to cleavage. TRMT1 proteolysis results in reduced tRNA binding and elimination of tRNA methyltransferase activity. We also determined the structure of an Mpro-TRMT1 peptide complex that shows how TRMT1 engages the Mpro active site in an uncommon substrate binding conformation. Finally, enzymology and molecular dynamics simulations indicate that kinetic discrimination occurs during a later step of Mpro-mediated proteolysis following substrate binding. Together, these data provide new insights into substrate recognition by SARS-CoV-2 Mpro that could help guide future antiviral therapeutic development and show how proteolysis of TRMT1 during SARS-CoV-2 infection impairs both TRMT1 tRNA binding and tRNA modification activity to disrupt host translation and potentially impact COVID-19 pathogenesis or phenotypes.
Deregulation mechanisms and therapeutic opportunities of p53-responsive microRNAs in diffuse large B-cell lymphoma
Authors: Voropaeva Elena N. , Orlov Yuriy L. , Loginova Anastasia B. , Seregina Olga B. , Maksimov Vladimir N. , Pospelova Tatiana I.
Published: January 2025

Here, we have discussed the molecular mechanisms of p53-responsive microRNAs dysregulation in response to genotoxic stress in diffuse large B-cell lymphoma (DLBCL) patients. The role of micro ribonucleic acids (microRNAs) in p53-signaling cellular stress has been studied. MicroRNAs are the small non-coding RNAs, which regulate genes expression at post-transcriptional level. Many of them play a crucial role in carcinogenesis and may act as oncogenes or suppressor of tumor growth. The understanding of the effect of p53-responsive microRNA dysregulation on oncogenesis achieved in recent decades opens wide opportunities for the diagnosis, prediction and of microRNA-based cancer therapy. Development of new bioinformatics tools and databases for microRNA supports DLBCL research. We overview the studies on the role of miRNAs in regulating gene expression associated with tumorigenesis processes, with particular emphasis on their role as tumor growth-suppressing factors. The starting point is a brief description of the classical microRNA biogenesis pathway and the role of p53 in regulating the expression of these molecules. We analyze various molecular mechanisms leading to this dysregulation, including mutations in the TP53 gene, DNA methylation, changes in host-genes expression or microRNA gene copy number, mutations in microRNA and microRNA biogenesis genes.
Trunk muscle behaviors during the full-cycle stoop and squat lifting tasks
Authors: Pan Fumin , Wang Wei , Kong Chao , Lu Shibao
Published: January 2025

Background

Lifting is generally considered as a risk factor for low back pain. A thorough investigation of the muscle function during lifting is essential for a better assessment of the potential risk of muscle impairment and towards improvements in lifting strategy. We aimed to compare the activities of the trunk muscles between the stoop and the squat lifting tasks.

Methods

A surface electromyography device was used to measure the muscle activity during the full-cycle squat and the stoop lifting tasks of a 5-kg weight. Each task was divided into four stages: stage 1 was bending forward to reach the weight, stage 2 was lifting the weight up, stage 3 was lowering the weight down, and stage 4 was returning to the upright position. The maximum electromyographic (EMG) activities among different tasks and different stages were compared. Eighteen males aged 20–35 years without low back pain were included, with a mean age of 26.55 ± 2.12 years, body height of 175.18 ± 4.29 cm, body weight of 69.27 ± 4.29 kg, and BMI of 22.56 ± 0.87 kg/m2.

Results

During stoop lifting, the median values of the absolute EMG of the left multifidus were 53.96, 70.32, 51.08 and 64.14 uV from stage 1 to stage 4, which were all non-significantly lower than those during squat lifting for 79.84, 103.64, 71.72 and 95.72, respectively (P > 0.05). The absolute EMG was greatest during stage 2, then during stage 4, stages 1 and 3 came next (Effect size = 0.879, P < 0.001). The median values of the normalized EMG of each muscle during stoop lifting were also non-significantly lower than those during squat lifting at each stage (P > 0.05). The normalized EMG was also greatest during stage 2, then during stage 4, and was lowest during stages 1 and 3 (Effect size = 0.932, P < 0.001).

Conclusion

The trunk muscles were similarly activated during squat and stoop lifting. During lowering the weight down, the trunk muscles were less activated than during extension to the upright position without weight in hands. These results could help to develop subject-specific strategies for lifting tasks to prevent or alleviate occupational low back pain.
A Narrative Review on Microbial Response to Antibiotic Stress: Mechanisms of Resistance and Adaptation
Authors: Khan Nazia
Published: January 2025

Microbial populations are constantly exposed to antibiotic pressures, which drive the evolution of diverse resistance mechanisms. This paper explores the complex responses of microbes to antibiotic stress, focusing on genetic and phenotypic adaptations. Key resistance mechanisms include Horizontal Gene Transfer (HGT), mutations leading to target site modification and the activation of efflux pumps. Additionally, the paper examines how microbial communities adapt to prolonged exposure through biofilm formation and altered metabolic pathways. Understanding these processes is crucial for developing strategies to combat antibiotic resistance and designing next-generation antimicrobial therapies.
A Case of Variceal Bleeding with Cricopharyngeal Web: A Challenge to Variceal Band Ligation
Authors: Kranthi Dandi , Banerjee Debabrata , Dahale Amol S , Karad Abhijeet , Bade Yogesh
Published: January 2025

Acute Variceal Bleeding (AVB) is a life-threatening complication of portal hypertension that necessitates prompt and effective intervention. Endoscopic Variceal Ligation (EVL) is the primary treatment modality; however, anatomical variations, such as oesophageal webs, can impede the procedure. The present report describes a 40-year-old female patient presenting with haematemesis and melena. Endoscopy confirmed active variceal bleeding, but the advancement of an endoscope equipped with a band ligator was obstructed by a cricopharyngeal web, rendering EVL unfeasible. Haemostasis was initially achieved using sclerotherapy with sodium tetradecyl sulfate. Subsequent endoscopic dilation of the web with a Controlled Radial Expansion (CRE) balloon facilitated successful band ligation. The present case underscores the importance of early recognition of anatomical anomalies and highlights the need for adaptable strategies in the management of AVB, particularly in emergent settings where time is critical. The clinical implications are significant, as the case highlights a previously unreported barrier to EVL in the form of a cricopharyngeal web. It emphasises the necessity for endoscopists to consider anatomical variations when standard procedures fail. The present report contributes to the literature by documenting an unusual clinical scenario that required modification of the standard therapeutic approach to achieve haemostasis in a life-threatening situation.
Examining the Determinants of User Stickiness in a Mobile Video Application: A Flow Theory Perspective
Authors: Gary Joston
Published: January 2025

This study investigates the determinants of user stickiness in short video applications through the lens of Flow Theory. A research model is developed to examine the influence of platform-specific factors, namely perceived personalization, design characteristics, and telepresence, on user stickiness, mediated by perceived control and flow experience. The study employs an online survey methodology, collecting data from 761 users of short video applications in China. Structural equation modeling is used to analyze the data and test the hypothesized relationships. The findings reveal that perceived personalization, design characteristics, and telepresence positively influence perceived control and flow experience, which in turn positively affect user stickiness. The study contributes to the literature by extending Flow Theory to the context of short video applications and providing empirical evidence for the role of platform-specific factors in driving user stickiness. Practical implications for short video platform operators are discussed, highlighting the importance of personalization, design, and telepresence in fostering user engagement and retention.
Assessment of Antisickling and Antioxidant Properties of Mucuna pruriens and Gossypium hirsutum Leaves
Authors: Otoe Anthonia Iyekoretin , Nwogoh Benedict , Ogbeifun David E , Iyekowa Osaro , Okieimen Felix E
Published: January 2025

Aim: In this study, the antioxidant and antisickling properties of Mucuna pruriens and Gossypium hirsutum leaves were determined.
Study Design: Experimental studies.
Place and Duration of Study: A patient with confirmed sickle cell disesase blood was got from the hematology unit of University of Benin Teaching Hospital, Benin City. Edo State. The procedure was carried out for 2 months.
Methods: Fresh Gossypium hirsutum and Mucuna pruriens leaves were obtained, identified, and then finely ground after being dried at room temperature.  To obtain the extracts, five hundred grams (500g) of both leaves were macerated in one thousand milliliters (1000ml) of methanol solvent. The Ferric Reducing Antioxidant Power (FRAP) assay was used to determine the antioxidant activity of the extract.
In-vitro method of sickle cell hemoglobin polymerization assay was used in determining the antisickling properties of the plant extracts. A Patient with confirmed sickle cell disease (HbSS), at the University of Benin Teaching Hospital, Nigeria participated in this study by providing blood samples. The samples were collected when the patient was in a non-crisis state and was stored in sodium EDTA bottles. The samples were thoroughly mixed, and used immediately for analysis.
Results: The methanolic extracts of Gossypium hirsutum and Mucuna pruriens showed antioxidant property which varied somewhat at 85.9 ±0.05% for Mucuna pruriens and 87.4 ±0.04% for Gossypium hirsutum and tendered to be higher for Gossypium hirsutum leaf extract than for Mucuna pruriens leaf extract. The antioxidant property of the plant extract compared favorably with value up to 88 ±0.02% of that of reference, ascorbic acid.  The plant extract showed antisickling effect that was both concentration of the plant extract and duration of application dependent when compared with the negative control, 17% sickling in the untreated HbSS sample, and 1% sickling with the reference p-hydroxybenzoic acid treated samples, the values marked antisickling effect.
Conclusion: The results of this research reveal that the methanolic extract of M. pruriens and G. hirsutum leaves have high antisickling and antioxidant properties.
Biochemical and Hematological Markers as Predictors of Severity in Pediatric Dengue: A Case Series Analysis
Authors: PG Vijayakumar , Gopalakrishnan Rahul , S Seethalakshmi , W Lydia Jeris
Published: January 2025

Dengue fever is a mosquito-borne viral illness caused by the dengue virus with a spectrum of presentations ranging from mild febrile illness to severe life-threatening conditions such as Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Early identification of severe cases is crucial to reduce morbidity and mortality especially in vulnerable pediatric populations. This case series presents four pediatric patients with varying degrees of dengue severity emphasizing the role of biochemical and hematological parameters in assessing disease severity and guiding clinical management. Parameters such as ferritin, LDH, lactate, liver enzymes (AST, ALT), platelet count and coagulation markers (PT, APTT) and PCV were observed to correlate with disease severity and recovery. The normalization of these markers with treatment highlighted their utility in monitoring patient progress. These findings reinforce the importance of incorporating a broad biochemical assessment in dengue management protocols to optimize patient outcomes.
Comparing Effects of Legume Intercropping and Green Leaf Manuring on Performance of Maize and Residual Soil Properties
Authors: Shrestha Karuna , Gautam Sonu , Pandit Amir , Shrestha Aman , Shrestha Milan , Poudel Gayatri , Ghimire Ramesh Kumar
Published: January 2025

In Nepal, there is a significant disparity between demand and supply for maize. Given that most smallholder farmers lack resources and hence cannot afford to purchase the necessary quantity of fertilizer. Hence, the use of different legumes intercropping and green leaf manure incorporation could be alternatives for increasing maize yield and enhancing soil properties. A field experiment was conducted at Lamjung Campus, Sundarbazzar, from March to July 2023 to study the response of maize to different legumes intercropping and green leaf manure incorporation and its effect on soil residual nutrients. The experiment was laid out in a randomized complete block design with five treatments (maize as a control, cowpea + maize, French bean + maize, soybean + maize, and green leaf manure + maize) and four replications. Nitrogen fertilizer was applied 60 kg ha-1 (half of the recommended dose), phosphorous and potassium were applied 15 kg ha-1 and 10 kg ha-1 (one-fourth of the recommended dose) respectively as its availability was already high in the soil. All the maize yield-related attributes were higher (P <0.05) in intercropping as compared to control. Significantly higher maize yield was obtained in green leaf manuring (2.21 times more than control), followed by soybean, cowpea, and French bean with 0.65, 0.25, and 0.09 times higher than control. In addition, nitrogen (2.25%) and organic matter (4.8%) were found higher in green leaf manure than others. It can be concluded that the input of green leaf manure to the soil enhances the yield of maize and soil properties.
Impact of Foliar Application of Fe, Zn and B in Combination with Mg on Growth, Yield and Quality of Acid Lime (Citrus aurantifolia Swingle) under Alfisols of Andhra Pradesh, India
Authors: Reddy A. Ramanjaneya , Prathap B. , Sreedhar D. , Reddy G. Chandramohan
Published: January 2025

A Field experiment was conducted on well established four years old orchard of acid lime at Citrus Research Station, Petlur, Dr. YSR Horticultural University, Andhra Pradesh during 2012-2018 to investigate the effect of micronutrient fertilization on the growth, yield and quality of acid lime cv. CRS-1. The experiment was laid out in a randomized block design with seven different combinations of three micronutrients (Fe 0.5%, Zn 0.5% and B 0.1%) in combination with Mg 0.2% and a control imposed each treatment on five plants. Foliar application was done during before flowering, late bloom stage and berry sized fruit stage. The result displayed that combined application of iron @ 0.5%, zinc @ 0.5%, boron @ 0.1% and Mg @ 0.2% recorded highest fruits per tree (618.33) and fruit yield (22.60 kg per tree), and improved fruit physical and chemical parameters like fruit weight, fruit diameter, juice %, total soluble solids, ascorbic acid, acidity % etc. The leaf nutrient content in acid lime leaf was significantly altered plants treated with combined application of Fe, Zn, B and Mg, i.e. T8. From these results, it concluded that combined foliar application of Mg, Fe, Zn and B is not only most effective for getting higher production and also better quality fruits of Acid lime under Alfisols of Andhra Pradesh.
Effects of Gender Difference on Fast Food Consumption among Associate Degree Students
Authors: İçöz Aysel , Kayişoğlu Serap
Published: January 2025

Aims: This study aims to evaluate the attitudes of associate degree students about fast food and to determine the effect of gender.
Study Design: Students who voluntarily agreed to participate in the study were included. Within the scope of the research, the questionnaire was conducted using a simple random sampling method to determine the students' consumption of ready-to-eat food. Questionnaire questions were developed based on the studies in the literature and organised by the purpose of the research.
Place and Duration of the Study: The survey was conducted face to face with 326 students (approximately 15 students from each program) studying in 10 different departments and 20 programs of Tekirdağ Namık Kemal University Technical Vocational School in the 2022-2023 fall semester.
Methodology: Before starting the questionnaire, the clarity and validity of the questions were tested with 10 subjects to make necessary corrections when necessary. In the questionnaire, students were asked about their height-weight values and Body Mass Index (BMI) was calculated. Descriptive statistics of the data obtained from the study were evaluated by Crosstabs analysis and Chi-square (χ2) independence tests in the SPSS ver.18 statistical package program.
Results: According to the study results, statistical significance was found in the following dimensions related to gender factor: BMI (**P<0.01), housing status (*P<0.05), frequency of consumption (*P<0.05), reason for consuming ready-to-eat food (*P<0.05), favorite ready-to-eat food (**P<0.01), portions consumed at meals (**P<0.01), finding portions sufficient (**P<0.01), comments about nutritional value (**P<0.01), and thoughts about hygiene (**P<0.01).
Conclusion: Understanding the factors affecting fast food consumption can contribute to raising awareness among students about balanced nutrition and developing nutrition strategies. Studies that investigate and understand these factors may be important for companies in the sector to follow consumer preferences and trends. New studies should be conducted among associate degree students, including students from different universities. To these studies; Factors such as family income level, whether the student receives a scholarship, health information, stress factor, social environment, advertising and social media use, food options on campus, and physical activity should be included.
Estimation of Some Heavy Metals in Different Types of Canned Tuna in the Egyptian Market
Authors: El-Senousi Naglaa A. , Ibrahim Ibrahim M. , Elkordy Mai M. , El-Hamed Shaimaa E. Abd
Published: January 2025

Fish is a high-quality food, but it can contain toxic heavy metals that accumulate in its tissues, posing a risk to human health as it is at the end of the food chain. These metals threaten public health due to their toxicity and ability to accumulate in tissues, leading to the depletion of essential nutrients, psychological and social problems, weakened immunity, delayed growth, increased risk of gastrointestinal cancer, and disabilities resulting from malnutrition. This study aimed to measure and compare the content of heavy metals (lead, cadmium, nickel, mercury, tin, copper, manganese and chromium) in five brands of canned tuna and its three types (shredded, chunks, and one-piece) in the Egyptian market. Heavy metal concentrations were generally below safe limits except for nickel in one of the samples, but HQ (the target hazard quotient) and HI (the total target hazard quotient) still showed no significant health risk. Based on the results, it can be concluded that no major health risks were identified for consumption of canned tuna from these five brands in the Egyptian market.
Effect of Germination, Drying, Milling and Sieving on the Nutritional Quality of Foxtail Millet (Setaria italica)
Authors: Santhosh Aiswarya Velekat , T S Asif Ali , B Rajunaik , B Ramachandra
Published: January 2025

Aims: The study aimed to evaluate the effect of germination, drying, milling and sieving on the protein and phytate content of foxtail millet
Study Design:  This was an experimental, laboratory-based study.
Place and Duration of Study: The study was conducted at the Department of Dairy Microbiology, Dairy Science College, Hebbal, Bengaluru, Karnataka, India, between January 2024 and October 2024.
Methodology: Soaked foxtail millet (Setaria italica) was germinated at different time-temperature combinations (20, 25 and 30°C for 24, 48 and 72 hours). Germination was optimized based on its effects on reducing phytate content and enhancing protein content. Post-germination, the millet was dried at 20°C, 25°C, and 30°C to a final moisture content of 5–7%, ensuring uniform moisture reduction while preserving nutritional quality. The optimum drying temperature was selected based on its influence on protein and phytate levels. The dried millet was milled into fine flour and sieved through a 150-micron sieve to achieve uniform particle size. The protein content was determined using the micro-Kjeldahl method, while phytate content was estimated via titration with ferric chloride. Statistical analysis was performed using ANOVA, with a critical difference (CD) at a 5% significance level used to determine significance among treatments.
Results: Germination at 30°C for 48 hours resulted in the highest protein content (14.76%) and significantly reduced phytate levels (1.59 mol/kg). Drying at 30°C for 48 hours achieved a moisture content of 6.32%, with protein content increasing to 15.10% and phytate content slightly rising to 1.65 mol/kg. Milling and sieving further reduced both protein (13.23%) and phytate content (1.40 mol/kg), with a statistically significant reduction observed only in phytate levels.
Conclusion: Germination at 30°C for 48 hours significantly enhanced protein content and reduced phytate levels in foxtail millet, while drying and sieving processes further optimized nutritional quality. These findings highlight the potential of tailored processing techniques to improve the functional and nutritional value of millet-based products.
Potential of Insect Gut Microbes in Advancing Renewable Energy Production: A Review
Authors: Prasanna Malireddi , Sarangi Satyabrata , Narwade D. K. , Thanuja Adama , Chandak Shubham , Mohapatra Rashmi , Jena Jay Prasad , Adhikari Bhubanananda
Published: January 2025

The growing global demand for sustainable energy solutions has intensified the exploration of novel biological systems for renewable energy production. Among these, insect gut microbes have emerged as a promising resource due to their unique ability to degrade lignocellulosic biomass, a major component of plant cell walls. The metabolic potential of insect gut microbiota and their applications in bioenergy production, emphasizing the enzymatic and microbial pathways that enable the conversion of plant biomass into biofuels such as bioethanol, biodiesel, methane, and hydrogen. Termites, beetles, cockroaches, and grasshoppers serve as key models, with their gut symbionts producing cellulases, hemicellulases, and lignin-degrading enzymes critical for biomass hydrolysis. Fermentation pathways in these microbial consortia allow for the efficient production of short-chain fatty acids, hydrogen, and methane under anaerobic conditions. Current research also focuses on the integration of insect gut microbiomes with advanced biotechnologies, including metagenomics and synthetic biology, to uncover novel enzymes and optimize microbial consortia for industrial applications. Significant challenges persist, including the difficulty of cultivating specific gut microbes in vitro, enzyme instability under industrial conditions, and ethical concerns related to bioprospecting. These limitations necessitate further advances in omics technologies, protein engineering, and microbial ecology. The development of hybrid bioenergy systems that combine insect gut microbes with algae, solar, or wind technologies, and the expansion of research to include lesser-studied insect species with untapped metabolic capabilities. Policy frameworks and interdisciplinary research funding are crucial for advancing this field and ensuring the ethical and sustainable use of microbial resources.
Pharmacokinetics of Enrofloxacin in Rohu (Labeo rohita): A Comparison of Bath, Intramuscular, and Oral Administration
Authors: Sreechandan Abhishek , Kalaiselvi L. , Hemamalini Nallaiah , Suresh E. , Shanmugam S. A. , Sriram P.
Published: January 2025

Enrofloxacin, fluoroquinolone antibacterial drug is widely used in aquaculture for the control of infectious diseases. Understanding the pharmacokinetics of enrofloxacin in Labeo rohita is critical for developing optimized therapeutic strategies so as to maximize efficacy and to minimize the emergence of resistant pathogens. Hence, the present study was aimed at studying the pharmacokinetics of enroflocacin in rohu fish (Labeo rohita). The pharmacokinetics of enrofloxacin was studied after single intramuscular or oral administration at 10 mg/kg body weight and following bath exposure at the concentration of 5 mg/L for 5 h. Blood samples were collected at predetermined time intervals. The plasma concentrations of enrofloxacin were determined by high performance liquid chromatography. The pharmacokinetic parameters were analyzed using a non-compartmental method. Cmax and Tmax varied depending on the route of administration, with intramuscular administration resulting in highest plasma concentration in short Tmax indicating rapid and better absorption.  Similarly, AUC0-∞ was found to be highest (166.51 μg.h.mL-1) following intramuscular administration indicating higher total systemic drug exposure compared to oral and bath exposure. Elimination half-life (t1/2) was found to be 43.31, 34.65, and 21.66 h following intramuscular, oral, and bath exposure, respectively. Intramuscular administration provided the highest plasma concentrations and the longest elimination half-life, suggesting its suitability for sustained treatment efficacy. The volume of distribution (Vd/F) was found to be maximum after oral administration (6.52 L/kg) as compared to the intramuscular route (3.75 L/kg) and bath exposure (2.07 L/kg). The results of our study indicate that enrofloxacin was well absorbed, widely distributed, and slowly eliminated in rohu following all three routes of administration. Our results suggest that enrofloxacin administered intramuscularly (10 mg/kg), orally (10 mg/kg), or by bath immersion (5 mg/L for 5 h) achieves sufficient plasma concentrations to treat pathogens with MIC ≤ 0.3 μg/mL.
Marketing and Supply Chain Analysis of Mushroom Production in Udham Singh Nagar District of Uttarakhand, India
Authors: Biswas Parth , Dev Chandra , Chavan Sanket D , Ali Sk Asraful , Prajapati Sunil Kumar
Published: January 2025

The present study was designed with the prominent objective to evaluate marketing cost, margin, and efficiency. Samples of 60 mushroom growers were surveyed from two blocks of Udham Singh Nagar district. Based on the production, respondents were grouped into three categories viz. medium, small and marginal.  For the analysis, Acharya’s method of marketing efficiency and Garrett’s ranking was used. From the study it was revealed that, marketing cost of per kg of mushroom in channel I, II and III was found Rs. 2.25, Rs. 23.66 and Rs. 40.1, respectively. Due to the absence of intermediaries, channel I had the highest modified marketing efficiency (MME). The study stressed the need for establishment of marketing facilities as a pre-requisite to promote mushroom production and also ensure remunerative price. Addressing marketing issue like problem in delay payment, storage and transportation are the major problems which will help to embrace this venture on a long run by creating more revenue.
Influence of Sodium Silicate on the Properties of Wood Ash Blended Cement Concrete
Authors: Raheem Akeem Ayinde , Otolorin Samuel Ayooluwa , Adebiyi Samad Olaide
Published: January 2025

This study explores the impact of sodium silicate on wood ash blended cement concrete, which contributes significantly to CO2 emissions. This research holds great potential for the construction industry by aiding in the reduction of greenhouse gas emissions, conserving natural resources, and promoting waste minimization. The specific research objectives are Characterizing sand, granite, and wood ash, Producing concrete mixes with wood ash and sodium silicate (Na2SiO3) replacements of 0.5%, 1.0%, 1.5%, and 2.0%, and determine their compressive strength, workability, density, and water absorption, and Developing a Long Short Term Memory (LSTM) deep learning model to determine optimal proportions of cement, w/c ratio, wood ash, and sodium silicate to achieve target compressive strength, workability, water absorption, and density in concrete mixes. Concrete specimens were tested for compressive strength, workability, density, and water absorption. A Long Short Term Memory (LSTM) machine learning model is significant because it is designed to capture historical information of time series data and is suitable for predicting long-term nonlinear series. The LSTM predicted compressive strength based on inputs of cement, water/cement ratio, sodium silicate, and wood ash. Results indicated that sodium silicate significantly enhanced early compressive strength and workability, making this blend a viable option for sustainable construction where early strength is essential.
Online Service Quality Assessment in Higher Education: Development, Validation, and Implementation of an e-Tool
Authors: Pan Melinda P.
Published: January 2025

In pursuit of quality and excellence in educational institutions, it is increasingly important to identify demands and needs of stakeholders. Service quality has been identified as one such demand. The purpose of the current study was to develop and validate a quality service assessment tool based on SERVQUAL Model to describe the service quality in Higher Education Institution. The sources of data were the head and personnel of the different clusters of CBSUA. The data were treated statistically using weighted mean and rank. The weighted-mean scores for the five SERVQUAL dimensions across the offices of the University President, Research and Innovation, Administration and Finance, Business and External Affairs, and Academic Cluster demonstrates that respondents find all five dimensions to be appropriate for assessing the service quality of each office. The relatively high scores across all dimensions highlight the importance of Tangibility, Assurance, Reliability, Reliability, Responsiveness, and Empathy in shaping stakeholders' perceptions and expectations of service quality within an educational institution. While some dimensions hold greater importance for specific offices due to their unique roles and responsibilities, all dimensions are nonetheless essential for evaluating the overall service quality in each context. The findings align with previous research on the applicability of the SERVQUAL model across various contexts, including higher education institutions. Ultimately, the results underline the significance of maintaining a high level of service quality across all dimensions in each office, as this is crucial for ensuring stakeholder satisfaction and fostering a positive reputation for the university.
Enhancing Mass and Yield Product of Propylene Glycol Production through Glycerol Hydrogenolysis
Authors: Arifin Dhea Wahyu Amanda , Firdaus Luthfi Maulana , Huda Muhammad Danil , Abdat Muhammad Saefillahil , Indriani Navira Amalia
Published: January 2025

The chemical industry in Indonesia continues to experience significant growth in both innovation and technology. One of the significant areas of improvement is in supporting materials, exemplified by propylene glycol. The process of producing propylene glycol from glycerol involves hydrogenolysis. The hydrogenolysis process of propylene glycol is the reaction of glycerol with hydrogen gas under specific conditions. The effects of process innovation or modification with the aim of enhancing mass efficiency and yield of propylene glycol. Methods to increase mass efficiency and yield using the Aspen HYSYS V11 simulator tool and implemented effectively. From the process modifications that have been implemented, it can be concluded that this design is quite effective as it mass and yields more efficiency, with one notable improvement being mass efficiency of propylene glycol from 7304 ton/year to 10012 ton/year and the percentage yield of propylene glycol in the final product increasing from 70% to 98%. Copyright © 2024 by Authors, Published by Universitas Diponegoro and BCREC Publishing Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).
Evidências e Perspectivas do Uso de 𝛽-glucana como Agente Imunomodulador
Authors: Diniz Susana Nogueira , Okuyama Cristina Eunice , Silva Valeria Lima da , Costa Samara Canazza Bezerra
Published: January 2025

O objetivo do estudo foi realizar uma análise prospectiva para identificar as principais tendências na utilização e na inovação do polissacarídeo 𝛽-glucana de diferentes origens como agente imunomodulador. Foram utilizadas como fonte de informação bulas de produtos com 𝛽-glucana e imunidade à venda em sites de e-commerce e a descrição de patentes levantadas pelo termo “beta-glucana” na base de patentes nacional. Este estudo identificou 60 produtos contendo 𝛽-glucanas de diferentes fontes, comercializados por empresas do setor alimentício, farmacêutico, saúde e bem-estar e dermocosmético, para uso humano e veterinário, na forma de cápsula, comprimido, líquido e pó. O levantamento patentométrico mostrou que necessidades humanas, clínica médica ou veterinária, agricultura e criação animal, por meio da produção de alimentos, fibras alimentares e tratamento de doenças, foram as utilidades mais citadas nas invenções, destacando-se três patentes relacionadas ao sistema imune. Este estudo permitiu mapear possibilidades de mercado para o desenvolvimento de produtos inovadores contendo 𝛽-glucana.
Motivos mais Recorrentes que Causam o Indeferimento de Pedidos de Patente das Universidades Brasileiras
Authors: Ferreira Frederico Aldecoa , Filgueiras Rogério de Andrade
Published: January 2025

Para  obter patente no Brasil, é necessário que o pedido seja submetido ao exame técnico do Instituto Nacional da Propriedade Industrial (INPI) que avalia se o pedido cumpre com os requisitos legais dispostos na Lei de Propriedade Industrial (LPI). Nesse sentido, o objetivo deste artigo foi analisar indeferimentos publicados entre 2020 e 2022 para pedidos de patente de universidades, investigando os motivos pelos quais tais pedidos foram indeferidos. O procedimento metodológico empregou pesquisa documental por meio da análise das Revistas da Propriedade Industrial (RPIs) publicadas pelo INPI entre 2020 e 2022, identificando 927 indeferimentos publicados para pedidos de patente de universidades nesse período. Verificou-se que a ausência de atividade inventiva é a causa mais frequente de indeferimento, seguida de falta de clareza e/ou fundamentação das reivindicações e ausência de novidade.
Prospecção Científica e Tecnológica de Aplicativos de Classificação da Água e Caracterização do Solo na Agricultura
Authors: Rodrigues Edemir de Carvalho , Silva Paula Tereza de Souza e
Published: January 2025

No semiárido brasileiro, a escassez de água de boa qualidade leva muitos agricultores a utilizarem água salina para irrigação, o que pode comprometer o desenvolvimento das culturas em ambientes salinos. Com a Agricultura 4.0, a adoção de tecnologias tornou-se essencial para otimizar a irrigação. Esta pesquisa buscou identificar inovações e aplicativos que fornecem recomendações sobre a classificação da qualidade da água e a caracterização do solo. Foram analisadas fontes como INPI, Google Play Store, Apple Store, Scopus, SciELO, IEEE Xplore, PatentScope®, Espacenet® e Questel Orbit®, utilizando palavras-chave como aplicativo, agricultura, água, solo, software, mobile, app, water e agriculture, com conectivos "AND" e "OR". A pesquisa revelou uma carência de aplicativos móveis nesse segmento, destacando-se apenas um aplicativo da Universidade Federal Rural do Semiárido (Ufersa) e um protótipo da International Business Machines Corporation (IBM). A escassez de soluções tecnológicas evidencia a necessidade de mais desenvolvimento no setor, especialmente para apoiar agricultores com pouco acesso à assistência técnica na agricultura familiar.
Patentes em Instituições Federais de Ensino Superior no Estado do Paraná
Authors: Flôr Priscila Grazielle , Silva Fabrízio Meller da
Published: January 2025

As Universidades possuem um importante papel como agentes geradores e difusores do conhecimento científico e tecnológico, contribuindo fortemente para a geração de inovações. A proteção da Propriedade Intelectual obtida no ambiente acadêmico ocorre por meio das patentes, mecanismo único de transferência do conhecimento tecnológico ao setor privado e um forte instrumento de renovação tecnológica. Ultimamente, pesquisas envolvendo a análise do portfólio de patentes das universidades se intensificaram na literatura. Assim, este estudo teve como propósito apresentar um levantamento dos depósitos de patentes realizados pelas Instituições de Ensino Superior mantidas pelo Governo Federal, localizadas em território Paranaense, cujos resultados indicaram uma expansão da produção intelectual, especialmente em relação à UTFPR e à UFPR, instituições que estão consolidadas há mais tempo no Estado. Esta é uma pesquisa de natureza exploratória e descritiva, instrumentalizada a partir de dados obtidos em pesquisas efetuadas junto ao Instituto Nacional da Propriedade Industrial (INPI).
Tecnologias Imunológicas no Desenvolvimento de Vacinas Contra Leishmaniose Humana (LH): uma prospecção científica e tecnológica
Authors: Silva Junior Fábio Feitosa , Sousa Ananda Lins Leite de , Gaspar Livia Maria Amorim Costa
Published: January 2025

A leishmaniose manifesta-se em três formas: Leishmaniose Cutânea (LC), Leishmaniose Visceral (LV) e Leishmaniose Mucocutânea (LMC). Essa doença afeta mais de um milhão de pessoas anualmente, com populações em áreas endêmicas ultrapassando um bilhão de indivíduos, e apresenta uma fase clínica grave e atualmente não dispõe de uma medida profilática eficaz, como as vacinas. O presente trabalho teve como objetivo realizar uma prospecção científica e tecnológica das patentes relacionadas às vacinas candidatas para a imunização global. Foram utilizados os descritores “Leishmaniasis”, “Vaccines”, “Leishmaniasis Vaccines” e “Development Vaccine”. A prospecção identificou resultados promissores, embora majoritariamente em fase in silico e in vivo em camundongos. Conclui-se que, com a contínua recuperação econômica pós-pandemia, os avanços tecnológicos e as novas ferramentas conduzidas por inteligência artificial, espera-se um progresso significativo nesses estudos, com potencial evolução para a fase de testes em humanos.
Aplicativos Móveis sobre Oxigenoterapia: revisão de patentes
Authors: Almeida Eliene , Dória Grace Anne Azevedo , Gomes Ana Beatriz Cruz Barbosa
Published: January 2025

Esta pesquisa buscou realizar uma revisão do estado da técnica sobre aplicativos e softwares direcionados à oxigenoterapia, por meio da prospecção de registros de softwares e patentes. Trata-se de um estudo exploratório, de caráter descritivo, constituindo um recorte de dissertação de mestrado. Os dados foram obtidos por meio de buscas em bases de patentes e de informações em lojas de aplicativos de julho a dezembro de 2023, conforme modelo prévio adaptado. Foram encontrados 11 produtos, sendo seis patentes e cinco aplicativos que prometem auxiliar profissionais no uso, no treinamento, no monitoramento dos pacientes e na gestão do oxigênio. Todos foram desenvolvidos entre os anos de 2018 e 2023 na China, Malásia, Japão, Índia, Brasil, Estados Unidos. Foram identificadas lacunas, referentes a aplicativos e patentes de tecnologias destinadas ao manejo da oxigenoterapia em adultos, embasados em pesquisas acadêmicas. Essas lacunas representam oportunidade de contribuição sobre o uso seguro do oxigênio em adultos.
Análise Bibliométrica e de Patentes sobre Catalisadores Utilizados na Síntese de Fischer-Tropsch para Produção de Bioquerosene de Aviação
Authors: Castro Jorge Arce , Mascarenhas Artur José Santos , Melo Silvio Alexandre Beisl Vieira de , Hechavarría Yanier Sánchez
Published: January 2025

Este estudo examina a síntese de Fischer-Tropsch (FT) na produção de bioquerosene de aviação (BioQAV) – em inglês, Sustainable Aviation Fuel, SAF –, essencial para a sustentabilidade e a redução de emissões de CO₂ no setor aéreo. Desenvolvida por Franz Fischer e Hans Tropsch nos anos 1920, a síntese de FT converte gás de síntese em hidrocarbonetos líquidos usando catalisadores de ferro ou cobalto. Crucial para gerar combustíveis líquidos a partir de carvão, gás natural ou biomassa, a FT é relevante em lugares em que o petróleo é escasso ou caro. O estudo realiza uma análise bibliométrica e patentométrica sobre catalisadores FT, focando na produção de SAF. Foram revisados 1.236 artigos de 2003 a 2024, obtidos nas bases Scopus e Web of Science, com a China liderando em publicações científicas, seguida pelos EUA. A análise de 2.128 famílias de patentes na plataforma The Lens Patents destaca EUA e China como principais inovadores, evidenciando intensa pesquisa e desenvolvimento em catalisadores eficientes e sustentáveis para SAF.
A Pedagogia Histórico-Crítica e a Teoria Marxista da Dependência: primeiras aproximações para relacionar nossa formação social à educação no Brasil
Authors: Kaplan Leonardo
Published: January 2025

Este artigo tem como objetivo iniciar um programa de pesquisas de articulação entre as formulações da Pedagogia Histórico-Crítica (PHC) e da Teoria Marxista da Dependência (TMD). Essa tentativa para que estas teorias dialoguem compreende que, com os aportes da TMD, é possível aprofundar questões colocadas pela PHC. Entende-se a PHC como uma teoria pedagógica marxista, sem, no entanto, ser uma teoria acabada. Sendo esta uma pedagogia revolucionária pensada no contexto brasileiro, por que não debater a formação social brasileira? Como o país se insere na economia mundial e quais os rebatimentos disto para a educação escolar, em particular, nesta sociedade? Categorias como Estado dependente, superexploração do trabalho e transferência de valor são fundamentais nesse sentido.
“Chorei sem saber o que ensinar”: reformismo, plataformização e precarização do trabalho docente na educação básica
Authors: Sousa Júnior João Eudes Alexandre de
Published: January 2025

O objetivo deste artigo é discutir como as “inovações” oriundas dos reformadores empresariais da educação, através do incentivo à plataformização via TDIC, reforçam estratégias de reificação dos currículos escolares, acentuando a alienação da força de trabalho educativo, precarizando à docência. O referencial teórico-metodológico adotado centra-se na investigação documental, desvelando-se mecanismos de exploração do trabalho docente apoiados por três condicionantes: alienação do trabalho, robotização do ensino (plataformização) e hiperprodutividade. Conclui-se que a tendência privatista liberal para C&T ainda é dual e reprodutivista, distante da uma práxis transformadora.
A Utilização Das Redes Neurais Artificiais Para Avaliação Do Índice De Desempenho Da Logística Interna
Authors: Lima Orlem Pinheiro De , Baggio Daniel Knebel , Filho Wlademir Leite Correia
Published: January 2025

Objetivo: Este estudo investiga o uso de Redes Neurais Artificiais (RNAs) na análise do desempenho da logística
interna, comparando seu desempenho com métodos convencionais. O trabalho busca descrever os dados
logísticos, identificar fatores que afetam a eficiência e examinar como as RNAs podem otimizar processos em
cenários logísticos complexos. Além disso, avalia-se o impacto das RNAs na gestão de estoques e no fluxo de
materiais.
Originalidade/Valor: O estudo se destaca demonstrando que as RNAs superam as abordagens tradicionais,
oferecendo soluções mais precisas e adaptáveis. Ele preenche lacunas nas avaliações logísticas tradicionais, que
frequentemente sofrem com a subjetividade, apresentando um modelo dinâmico que se ajusta aos dados em tempo
real. A aplicação prática desta tecnologia promove maior eficiência operacional e redução de custos nas
atividades logísticas internas.
Design/Metodologia/Abordagem: Foi realizada uma análise comparativa entre métodos tradicionais e um
modelo de rede neural do tipo feedforward, implementado no MATLAB, para avaliar a logística interna de três
empresas. O treinamento da RNA foi feito com dados reais, utilizando o algoritmo de retropropagação para
minimizar os erros.
Resultados: As Redes Neurais Artificiais mostraram maior eficácia em termos de precisão e flexibilidade quando
comparadas aos métodos tradicionais, resultando em uma redução significativa nas margens de erro durante a
avaliação do desempenho logístico.
Contribuição/Implicação: A principal contribuição deste estudo está na utilização de RNAs para aprimorar a
avaliação da logística interna, superando as limitações dos métodos convencionais. As RNAs permitem que as empresas ajustem seus processos rapidamente, com base em previsões precisas, promovendo agilidade e eficiência
Impact Of The Ali Program In Promoting Innovation And Competitiveness In Micro And Small Enterprises In São Paulo State
Authors: Trevisanuto Tatiene Martins Coelho , Castro Jacqueline Aparecida Gonçalves Fernandes De , Justiniano Alexander Vinicius De Sousa , Ussuna Guilherme De Andrade , Satolo Eduardo Guilherme
Published: January 2025

The Local Innovation Agent (ALI) Program, promoted by SEBRAE, plays a fundamental role in disseminating a
culture of innovation and strengthening the competitiveness of micro and small enterprises (MSEs) in Brazil. The
analysis of the performance of ALI agents in the state of São Paulo highlights academic training and key skills,
at the same time as it identifies gaps and opportunities to improve professional training. The results show that
education in areas such as business administration, economics, and accounting is often associated with successful
performance in the role, although technical and practical training still presents deficiencies, especially in topics
such as digital tools, innovation management, and client relationships. In addition, 90% of agents consider
continuous training essential to keep up with innovative demands. The research highlights that the methodology
and operational practices of the program have a positive impact on the productivity and innovation of MSEs,
especially in organizational and marketing dimensions. However, improving training strategies focusing on live
training, practical experiences, and integration with market professionals can enhance and improve program
results. The study concludes that the ALI Program is a strategic initiative for the survival and sustainable growth of MSEs, emphasizing the need for gradual and continuous evolution of its structure to meet the specific demands of the market and the innovative ecosystem