bluuebunny/crossref_metadata_2025 huggingface dataset:
A Potent Leukocyte Transmigration Blocker: GT-73 Showed a Protective Effect against LPS-Induced ARDS in Mice
Authors: Blum Eliav , Margalit Raanan , Levy Laura , Getter Tamar , Lahav Ron , Zilber Sofia , Bradfield Paul , Imhof Beat A. , Alpert Evgenia , Gruzman Arie
Published: July 2021

We recently developed a molecule (GT-73) that blocked leukocyte transendothelial migration from blood to the peripheral tissues, supposedly by affecting the platelet endothelial cell adhesion molecule (PECAM-1) function. GT-73 was tested in an LPS-induced acute respiratory distress syndrome (ARDS) mouse model. The rationale for this is based on the finding that the mortality of COVID-19 patients is partly caused by ARDS induced by a massive migration of leukocytes to the lungs. In addition, the role of tert-butyl and methyl ester moieties in the biological effect of GT-73 was investigated. A human leukocyte, transendothelial migration assay was applied to validate the blocking effect of GT-73 derivatives. Finally, a mouse model of LPS-induced ARDS was used to evaluate the histological and biochemical effects of GT-73. The obtained results showed that GT-73 has a unique structure that is responsible for its biological activity; two of its chemical moieties (tert-butyl and a methyl ester) are critical for this effect. GT-73 is a prodrug, and its lipophilic tail covalently binds to PECAM-1 via Lys536. GT-73 significantly decreased the number of infiltrating leukocytes in the lungs and reduced the inflammation level. Finally, GT-73 reduced the levels of IL-1β, IL-6, and MCP-1 in bronchoalveolar lavage fluid (BALF). In summary, we concluded that GT-73, a blocker of white blood cell transendothelial migration, has a favorable profile as a drug candidate for the treatment of ARDS in COVID-19 patients.
Rosa spp. Extracts as a Factor That Limits the Growth of Staphylococcus spp. Bacteria, a Food Contaminant
Authors: Milala Joanna , Piekarska-Radzik Lidia , Sójka Michał , Klewicki Robert , Matysiak Bożena , Klewicka Elżbieta
Published: July 2021

Due to their richness of bioactive substances, rose hips are a valuable raw material for obtaining extracts with potential antimicrobial activity. The aim of the study was to determine the antagonistic potential of whole pseudo-fruit and flesh extracts of three Rosa sp. varieties against Staphylococcus spp. bacteria isolated as food contaminants. The biological material in this study consisted of seven strains of bacteria from the genus Staphylococcus. Two strains—Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 3270—were used as reference strains. The other five strains were food-derived isolates—S. epidermidis A5, S. xylosus M5, S. haemolyticus M6, S. capitis KR6, and S. warneri KR2A. The material was the pseudo-fruits of Rosa canina, Rosa pomifera Karpatia, and Rosa rugosa. The polyphenols were extracted from the fleshy part and the whole pseudo-fruit for all rose varieties. The tested preparations differed significantly in their polyphenol composition. The sum of polyphenols ranged from 28 862 to 35 358 mg/100 g of lyophilisate. The main groups of polyphenols found in the preparations were flavanols and ellagitannins. All of the tested extracts inhibited the growth of staphylococci at a concentration of 500 mg/mL. Rosa rugosa fruit extract showed the strongest antimicrobial properties among the studied extracts. For all the strains, the growth inhibition had a diameter of 20.3–29.0 mm. Moreover, six out of the seven tested strains showed the highest inhibition with the use of this extract. The MIC of rose extracts was in the range of 3.125–500 mg/mL and was strictly dependent on the bacterial species, the species of the rose, and the part of the fruit from which the extract was obtained. Correlations were assessed between the main groups of polyphenols in the extracts and their inhibition of bacterial growth. In the case of pseudo-fruit extracts, the inhibitory effect on bacterial growth positively correlated with the content of ellagitannins, and this effect was observed for almost all the tested strains. The results presented herein follow the current trend of minimising the use of chemical preservatives in food; from this point of view, rose extracts are very promising.
Betulin, a Newly Characterized Compound in Acacia auriculiformis Bark, Is a Multi-Target Protein Kinase Inhibitor
Authors: Ahmadu Augustine A. , Delehouzé Claire , Haruna Anas , Mustapha Lukman , Lawal Bilqis A. , Udobre Aniefiok , Baratte Blandine , Triscornia Camilla , Autret Axelle , Robert Thomas , Bulinski Jeannette Chloë , Rousselot Morgane , Simoes Eugénio Mélanie , Dimanche-Boitrel Marie-Thérèse , Petzer Jacobus P. , Legoabe Lesetja J. , Bach Stéphane
Published: July 2021

The purpose of this work is to investigate the protein kinase inhibitory activity of constituents from Acacia auriculiformis stem bark. Column chromatography and NMR spectroscopy were used to purify and characterize betulin from an ethyl acetate soluble fraction of acacia bark. Betulin, a known inducer of apoptosis, was screened against a panel of 16 disease-related protein kinases. Betulin was shown to inhibit Abelson murine leukemia viral oncogene homolog 1 (ABL1) kinase, casein kinase 1ε (CK1ε), glycogen synthase kinase 3α/β (GSK-3 α/β), Janus kinase 3 (JAK3), NIMA Related Kinase 6 (NEK6), and vascular endothelial growth factor receptor 2 kinase (VEGFR2) with activities in the micromolar range for each. The effect of betulin on the cell viability of doxorubicin-resistant K562R chronic myelogenous leukemia cells was then verified to investigate its putative use as an anti-cancer compound. Betulin was shown to modulate the mitogen-activated protein (MAP) kinase pathway, with activity similar to that of imatinib mesylate, a known ABL1 kinase inhibitor. The interaction of betulin and ABL1 was studied by molecular docking, revealing an interaction of the inhibitor with the ABL1 ATP binding pocket. Together, these data demonstrate that betulin is a multi-target inhibitor of protein kinases, an activity that can contribute to the anticancer properties of the natural compound and to potential treatments for leukemia.
N-Skatyltryptamines—Dual 5-HT6R/D2R Ligands with Antipsychotic and Procognitive Potential
Authors: Hogendorf Agata , Hogendorf Adam S. , Kurczab Rafał , Satała Grzegorz , Szewczyk Bernadeta , Cieślik Paulina , Latacz Gniewomir , Handzlik Jadwiga , Lenda Tomasz , Kaczorowska Katarzyna , Staroń Jakub , Bugno Ryszard , Duszyńska Beata , Bojarski Andrzej J.
Published: July 2021

A series of N-skatyltryptamines was synthesized and their affinities for serotonin and dopamine receptors were determined. Compounds exhibited activity toward 5-HT1A, 5-HT2A, 5-HT6, and D2 receptors. Substitution patterns resulting in affinity/activity switches were identified and studied using homology modeling. Chosen hits were screened to determine their metabolism, permeability, hepatotoxicity, and CYP inhibition. Several D2 receptor antagonists with additional 5-HT6R antagonist and agonist properties were identified. The former combination resembled known antipsychotic agents, while the latter was particularly interesting due to the fact that it has not been studied before. Selective 5-HT6R antagonists have been shown previously to produce procognitive and promnesic effects in several rodent models. Administration of 5-HT6R agonists was more ambiguous—in naive animals, it did not alter memory or produce slight amnesic effects, while in rodent models of memory impairment, they ameliorated the condition just like antagonists. Using the identified hit compounds 15 and 18, we tried to sort out the difference between ligands exhibiting the D2R antagonist function combined with 5-HT6R agonism, and mixed D2/5-HT6R antagonists in murine models of psychosis.
Single Origin Coffee Aroma: From Optimized Flavor Protocols and Coffee Customization to Instrumental Volatile Characterization and Chemometrics
Authors: Zakidou Panagiota , Plati Fotini , Matsakidou Anthia , Varka Evdoxia-Maria , Blekas Georgios , Paraskevopoulou Adamantini
Published: July 2021

In this study, the aroma profile of 10 single origin Arabica coffees originating from eight different growing locations, from Central America to Indonesia, was analyzed using Headspace SPME-GC-MS as the analytical method. Their roasting was performed under temperature–time conditions, customized for each sample to reach specific sensory brew characteristics in an attempt to underline the customization of roast profiles and implementation of separate roastings followed by subsequent blending as a means to tailor cup quality. A total of 138 volatile compounds were identified in all coffee samples, mainly furan (~24–41%) and pyrazine (~25–39%) derivatives, many of which are recognized as coffee key odorants, while the main formation mechanism was the Maillard reaction. Volatile compounds’ composition data were also chemometrically processed using the HCA Heatmap, PCA and HCA aiming to explore if they meet the expected aroma quality attributes and if they can be an indicator of coffee origin. The desired brew characteristics of the samples were satisfactorily captured from the volatile compounds formed, contributing to the aroma potential of each sample. Furthermore, the volatile compounds presented a strong variation with the applied roasting conditions, meaning lighter roasted samples were efficiently differentiated from darker roasted samples, while roasting degree exceeded the geographical origin of the coffee. The coffee samples were distinguished into two groups, with the first two PCs accounting for 73.66% of the total variation, attributed mainly to the presence of higher quantities of furans and pyrazines, as well as to other chemical classes (e.g., dihydrofuranone and phenol derivatives), while HCA confirmed the above results rendering roasting conditions as the underlying criterion for differentiation.
Evaluation of Cocaine Effect on Endogenous Metabolites of HepG2 Cells Using Targeted Metabolomics
Authors: Krokos Adamantios , Deda Olga , Virgiliou Christina , Gika Helen , Raikos Nikolaos , Aggelidou Eleni , Kritis Aristeidis , Theodoridis Georgios
Published: July 2021

Cocaine toxicity has been a subject of study because cocaine is one of the most common and potent drugs of abuse. In the current study the effect of cocaine on human liver cancer cell line (HepG2) was assessed. Cocaine toxicity (IC50) on HepG2 cells was experimentally calculated using an XTT assay at 2.428 mM. The metabolic profile of HepG2 cells was further evaluated to investigate the cytotoxic activity of cocaine at 2 mM at three different time points. Cell medium and intracellular material samples were analyzed with a validated HILIC-MS/MS method for targeted metabolomics on an ACQUITY Amide column in gradient mode with detection on a triple quadrupole mass spectrometer in multiple reaction monitoring. About 106 hydrophilic metabolites from different metabolic pathways were monitored. Multivariate analysis clearly separated the studied groups (cocaine-treated and control samples) and revealed potential biomarkers in the extracellular and intracellular samples. A predominant effect of cocaine administration on alanine, aspartate, and glutamate metabolic pathway was observed. Moreover, taurine and hypotaurine metabolism were found to be affected in cocaine-treated cells. Targeted metabolomics managed to reveal metabolic changes upon cocaine administration, however deciphering the exact cocaine cytotoxic mechanism is still challenging.
Intake of Potatoes Is Associated with Higher Diet Quality, and Improved Nutrient Intake and Adequacy among US Adolescents: NHANES 2001–2018 Analysis
Authors: Agarwal Sanjiv , Fulgoni Victor L.
Published: July 2021

Potatoes are nutrient rich white vegetables, however, research on their impact on public health is limited. The objective of this study was to provide updated evaluation of the cross-sectional association between potato consumption and diet quality, nutrient intake and adequacy. Twenty-four hour diet recall data from adolescents (n = 16,633; age 9–18 years) were used to assess intakes. Usual intakes of nutrients were determined using the National Cancer Institute method and diet quality was calculated using the Healthy Eating Index-2015 (HEI-2015) after adjusting for demographic factors. Consumers of potatoes (baked or boiled potatoes, mashed potatoes and potato mixtures, fried potatoes, and potato chips) had higher (p < 0.05) HEI-2015 total score and subcomponent scores for total vegetables, total protein foods, and refined grain than non-consumers. Consumers also had higher (p < 0.05) intake of energy, dietary fiber, protein, copper, magnesium, phosphorus, potassium, selenium, sodium, zinc, niacin, vitamin B6, vitamin C, vitamin K and total choline; and higher (p < 0.05) adequacy for protein, copper, magnesium, phosphorus, potassium, zinc, thiamine, niacin, vitamin B6, vitamin C, and vitamin K than non-consumers. In conclusion, adolescent potato consumption was associated with higher diet quality, nutrient intake, and adequacy and therefore encouraging their consumption may be an effective strategy for improving nutritional status.
Influence of a Pediatric Fruit and Vegetable Prescription Program on Child Dietary Patterns and Food Security
Authors: Saxe-Custack Amy , LaChance Jenny , Jess Jennifer , Hanna-Attisha Mona
Published: July 2021

Limited access to fresh foods is a barrier to adequate consumption of fruits and vegetables among youth, particularly in low-income communities. The current study sought to examine preliminary effectiveness of a fruit and vegetable prescription program (FVPP), which provided one USD 15 prescription to pediatric patients during office visits. The central hypothesis was that exposure to this FVPP is associated with improvements in dietary patterns and food security. This non-controlled longitudinal intervention trial included a sample of caregiver–child dyads at one urban pediatric clinic who were exposed to the FVPP for 1 year. Patients received one USD 15 prescription for fresh produce during appointments. A consecutive sample of caregivers whose children were 8–18 years of age were invited to participate in the study. Dyads separately completed surveys that evaluated food security and dietary behaviors prior to receipt of their first prescription and again at 12 months. A total of 122 dyads completed surveys at baseline and 12-month follow-up. Approximately half of youth were female (52%), and most were African American (63%). Mean caregiver-reported household food security improved from baseline to 12 months (p < 0.001), as did mean child-reported food security (p = 0.01). Additionally, child-reported intake of vegetables (p = 0.001), whole grains (p = 0.001), fiber (p = 0.008), and dairy (p < 0.001) improved after 12 months of exposure to the FVPP. This study provides evidence that pediatric FVPPs may positively influence food security and the dietary patterns of children.
Pro-Osteogenic Properties of Violina pumpkin (Cucurbita moschata) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures
Authors: Lambertini Elisabetta , Penolazzi Letizia , Pellielo Giulia , Pipino Caterina , Pandolfi Assunta , Fiorito Serena , Epifano Francesco , Genovese Salvatore , Piva Roberta
Published: July 2021

Traditional medicines rely mainly on use of plant extracts to mitigate or treat a wide range of disorders, including those that affect skeletal homeostasis. In this study, we investigated for the first time the potential pro-osteogenic effects of hexane, acetone and methanol extracts of the leaves of Cucurbita moschata, a very popular pumpkin cultivar in Western countries. We found that in Cucurbita moschata leaves, there are acetone-extractable substances—in particular, fatty acids such as 13-OH-9Z,11E,15E-octadecatrienoic acid (PU-13OH-FA), which is capable of both stimulating the function of human primary osteoblasts, which are responsible for bone formation, and inhibiting the differentiation of human osteoclasts, which are responsible for bone resorption. This dual effect was monitored by analyzing Runx2 expression, deposition of mineralized matrix, ALP activity, TRAP and actin ring staining respectively. This study suggests that bioactive chemicals from Cucurbita moschata leaves are potentially suitable as therapeutics for managing metabolic bone disorders such as osteoporosis and rheumatoid arthritis, and promoting tissue healing and functional recovery after bone fractures. The data we obtained increase knowledge on the biological activities of Cucurbita moschata, and in particular underline the potential benefits of consuming leaves which are a part of the plant currently little considered in the Western world.
Universal School Meals in the US: What Can We Learn from the Community Eligibility Provision?
Authors: Andreyeva Tatiana , Sun Xiaohan
Published: July 2021

Changes in school meal programs can affect well-being of millions of American children. Since 2014, high-poverty schools and districts nationwide had an option to provide universal free meals (UFM) through the Community Eligibility Provision (CEP). The COVID-19 pandemic expanded UFM to all schools in 2020–2022. Using nationally representative data from the Early Childhood Longitudinal Study: Kindergarten Class of 2010–2011, we measured CEP effects on school meal participation, attendance, academic achievement, children’s body weight, and household food security. To provide plausibly causal estimates, we leveraged the exogenous variation in the timing of CEP implementation across states and estimated a difference-in-difference model with child random effects, school and year fixed effects. On average, CEP participation increased the probability of children’s eating free school lunch by 9.3% and daily school attendance by 0.24 percentage points (p < 0.01). We find no evidence that, overall, CEP affected body weight, test scores and household food security among elementary schoolchildren. However, CEP benefited children in low-income families by decreasing the probability of being overweight by 3.1% (p < 0.05) and improving reading scores of Hispanic children by 0.055 standard deviations. UFM expansion can particularly benefit at-risk children and help improve equity in educational and health outcomes.
Atelerix algirus, the North African Hedgehog: Suitable Wild Host for Infected Ticks and Fleas and Reservoir of Vector-Borne Pathogens in Tunisia
Authors: Balti Ghofrane , Galon Clemence , Derghal Moufida , Souguir Hejer , Guerbouj Souheila , Rhim Adel , Chemkhi Jomâa , Guizani Ikram , Bouattour Ali , Moutailler Sara , M’ghirbi Youmna
Published: July 2021

Small wild mammals are an important element in the emergence and transmission of vector-borne pathogens (VBPs). Among these species, hedgehogs have been found to be a reservoir of VBPs and host of arthropod vectors. Surveillance of VBPs in wildlife and their arthropods are crucial in a one health context. We conducted an exploratory study to screen Atelerix algirus hedgehogs and their infesting ticks and fleas for VBPs using a high throughput microfluidic real-time PCR system. Tested biopsies from hedgehogs were found to be naturally infected by Theileria youngi, Hepatozoon sp., Ehrlichia ewingii, Coxiella burnetii, and Candidatus Ehrlichia shimanensis. Similarly, Haemaphysalis erinacei and Rhipicephalus sanguineus tick species were infected by Ehrlichia ewingii, Rickettsia spp., Rickettsia massiliae, Borrelia sp., Coxiella burnetii, Rickettsia lusitaniae and Anaplasma sp. Archaeopsylla erinacei fleas were infected by Rickettsia asembonensis, Coxiella burnetii, and Rickettsia massiliae. Co-infections by two and three pathogens were detected in hedgehogs and infesting ticks and fleas. The microfluidic real-time PCR system enabled us not only to detect new and unexpected pathogens, but also to identify co-infections in hedgehogs, ticks, and fleas. We suggest that hedgehogs may play a reservoir role for VBPs in Tunisia and contribute to maintaining enzootic pathogen cycles via arthropod vectors.
Structural and Functional Genomics of the Resistance of Cacao to Phytophthora palmivora
Authors: Mucherino Muñoz Jonathan Javier , de Melo Cláusio Antônio Ferreira , Santana Silva Raner José , Luz Edna Dora Martins Newman , Corrêa Ronan Xavier
Published: July 2021

Black pod disease, caused by Phytophthora spp., is one of the main diseases that attack cocoa plantations. This study validated, by association mapping, 29 SSR molecular markers flanking to QTL (Quantitative Trait Loci) associated with Phytophthora palmivora Butler (Butler) (PP) resistance, in three local ancient varieties of the Bahia (Comum, Pará, and Maranhão), varieties that have a high potential in the production of gourmet chocolate. Four SSR loci associated with resistance to PP were detected, two on chromosome 8, explaining 7.43% and 3.72% of the Phenotypic Variation (%PV), one on chromosome 2 explaining 2.71%PV and one on chromosome 3 explaining 1.93%PV. A functional domains-based annotation was carried out, in two Theobroma cacao (CRIOLLO and MATINA) reference genomes, of 20 QTL regions associated with cocoa resistance to the pathogen. It was identified 164 (genome CRIOLLO) and 160 (genome MATINA) candidate genes, hypothetically involved in the recognition and activation of responses in the interaction with the pathogen. Genomic regions rich in genes with Coiled-coils (CC), nucleotide binding sites (NBS) and Leucine-rich repeat (LRR) domains were identified on chromosomes 1, 3, 6, 8, and 10, likewise, regions rich in Receptor-like Kinase domain (RLK) and Ginkbilobin2 (GNK2) domains were identified in chromosomes 4 and 6.
Synthesis and Evaluation of the Antidepressant-like Properties of HBK-10, a Novel 2-Methoxyphenylpiperazine Derivative Targeting the 5-HT1A and D2 Receptors
Authors: Sałaciak Kinga , Malikowska-Racia Natalia , Lustyk Klaudia , Siwek Agata , Głuch-Lutwin Monika , Kazek Grzegorz , Popiół Justyna , Sapa Jacek , Marona Henryk , Żelaszczyk Dorota , Pytka Karolina
Published: July 2021

The increasing number of patients reporting depressive symptoms requires the design of new antidepressants with higher efficacy and limited side effects. As our previous research showed, 2-methoxyphenylpiperazine derivatives are promising candidates to fulfill these criteria. In this study, we aimed to synthesize a novel 2-methoxyphenylpiperazine derivative, HBK-10, and investigate its in vitro and in vivo pharmacological profile. After assessing the affinity for serotonergic and dopaminergic receptors, and serotonin transporter, we determined intrinsic activity of the compound at the 5-HT1A and D2 receptors. Next, we performed behavioral experiments (forced swim test, tail suspension test) to evaluate the antidepressant-like activity of HBK-10 in naïve and corticosterone-treated mice. We also assessed the safety profile of the compound. We showed that HBK-10 bound strongly to 5-HT1A and D2 receptors and presented antagonistic properties at these receptors in the functional assays. HBK-10 displayed the antidepressant-like effect not only in naïve animals, but also in the corticosterone-induced mouse depression model, i.e., chronic administration of HBK-10 reversed corticosterone-induced changes in behavior. Moreover, the compound’s sedative effect was observed at around 26-fold higher doses than the antidepressant-like ones. Our study showed that HBK-10 displayed a favorable pharmacological profile and may represent an attractive putative treatment candidate for depression.
Recent Advances in the Anti-Inflammatory Activity of Plant-Derived Alkaloid Rhynchophylline in Neurological and Cardiovascular Diseases
Authors: Geetha Rajeswari Gopal , Ramachandran Surya
Published: July 2021

Rhynchophylline (Rhy) is a plant-derived indole alkaloid isolated from Uncaria species. Both the plant and the alkaloid possess numerous protective properties such as anti-inflammatory, neuroprotective, anti-hypertensive, anti-rhythmic, and sedative effects. Several studies support the significance of the anti-inflammatory activity of the plant as an underlying mechanism for most of the pharmacological activities of the alkaloid. Rhy is effective in protecting both the central nervous system and cardiovascular system. Cerebro-cardiovascular disease primarily occurs due to changes in lifestyle habits. Many previous studies have highlighted the significance of Rhy in modulating calcium channels and potassium channels, thereby protecting the brain from neurodegenerative diseases and related effects. Rhy also has anticoagulation and anti-platelet aggregation activity. Although Rhy has displayed its role in protecting the cardiovascular system, very little is explored about its intervention in early atherosclerosis. Extensive studies are required to understand the cardioprotective effects of Rhye. This review summarized and discussed the various pharmacological effects of Rhy in neuro- and cardioprotection and in particular the relevance of Rhy in preventing early atherosclerosis using Rhy-loaded nanoparticles.
Ethyl Acetate Fraction and Isolated Phenolics Derivatives from Mandevilla moricandiana Identified by UHPLC-DAD-ESI-MSn with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction
Authors: Ferreira Leticia L. D. M. , Leão Valéria de F. , Melo Cinthya M. de , Machado Thelma de B. , Amaral Ana Claudia F. , Silva Leandro L. da , Simas Naomi K. , Muzitano Michelle F. , Leal Ivana C. R. , Raimundo Juliana M.
Published: July 2021

Endothelial dysfunction in obesity plays a key role in the development of cardiovascular diseases, and it is characterized by increased vascular tonus and oxidative stress. Thus, this study aimed to investigate the vasodilatory and antioxidant activities of Mandevilla moricandiana ethyl acetate fraction and subfractions. Vascular effects were investigated on aorta isolated from control and monosodium glutamate (MSG) induced-obese Wistar rats, and antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods. The ethyl acetate fraction (MMEAF) induced a concentration-dependent vasodilation on aortic rings through the NO pathway, with the involvement of histamine H1 and estrogen ERα receptors and showed potent antioxidant activity. In aorta of MSG obese rats, maximal relaxation to acetylcholine was increased in the presence of MMEAF (3 µg/mL), indicating that MMEAF ameliorated obesity-induced endothelial dysfunction. Quercetin and kaempferol aglycones and their correspondent glycosides, as well as caffeoylquinic acid derivatives, A-type procyanidin trimer, ursolic and oleanolic triterpenoid acids were identified in subfractions from MMEAF and seem to be the metabolites responsible for the vascular and antioxidant activities of this fraction.
Optimum Preparation Conditions for Highly Individualized Chitin Nanofibers Using Ultrasonic Generator
Authors: Zewude Dagmawi Abebe , Izawa Hironori , Ifuku Shinsuke
Published: July 2021

α-Chitin derived from crab shells was treated with 30% sodium hydroxide to prepare partially deacetylated chitin with a deacetylation degree of 36%. Partially deacetylated chitin nanofibers were prepared by applying weak ultrasonic energy generated by a domestic ultrasonic cleaner. The deacetylated chitin was easily disintegrated into nanofibers with the aid of electrostatic repulsion and osmotic pressure effect of amino cations on the chitin surfaces. The nanofibers were characterized in terms of yield, morphology, crystallinity, viscosity, and dispersibility. After a series of characterizations, ultrasonication with 45 kHz frequency and 20 min treatment was found to be the optimum conditions for obtaining fine nanofibers with a high yield.
Relationship between Total Phenolic Content, Antioxidant Capacity, Fe and Cu Content from Tea Plant Samples at Different Brewing Times
Authors: Dobrinas Simona , Soceanu Alina , Popescu Viorica , Carazeanu Popovici Ionela , Jitariu Daniela
Published: July 2021

The purpose of this study was to investigate the antioxidant capacity of different tea plant: mint, linden, chamomile, St. John’s wort, green and black tea in relation to total phenolic content. The antioxidant capacity of the tea infusions at different brewing times was determined using DPPH assay while the total phenolic content (TPC) was assessed using the modified Folin-Ciocalteu method. The results showed that there were significant statistical differences in antioxidant capacity depending on infusion time, according to one-way ANOVA analysis. Leaves used as components of tea infusions were analyzed by FAAS for their content of iron and copper in the dry product and in the infusion. The correlation between TPC and DPPH capacity of tea plant infusions was evaluated by Pearson correlation matrix. Total phenolics compounds content was positively and significantly correlated with DPPH capacity for all infusions time. Significant correlation was observed between TPC and the copper concentration (p < 0.05). Consequently, the correlations between the physicochemical parameters, TPC, DPPH capacity, Fe and Cu content suggested that the TPC may be a good indicator of the DPPH capacity in the tea infusions and also, suggested the influence of antioxidant compounds on mineral bioavailability.
Microfluidic Network Simulations Enable On-Demand Prediction of Control Parameters for Operating Lab-on-a-Chip-Devices
Authors: Böke Julia Sophie , Kraus Daniel , Henkel Thomas
Published: July 2021

Reliable operation of lab-on-a-chip systems depends on user-friendly, precise, and predictable fluid management tailored to particular sub-tasks of the microfluidic process protocol and their required sample fluids. Pressure-driven flow control, where the sample fluids are delivered to the chip from pressurized feed vessels, simplifies the fluid management even for multiple fluids. The achieved flow rates depend on the pressure settings, fluid properties, and pressure-throughput characteristics of the complete microfluidic system composed of the chip and the interconnecting tubing. The prediction of the required pressure settings for achieving given flow rates simplifies the control tasks and enables opportunities for automation. In our work, we utilize a fast-running, Kirchhoff-based microfluidic network simulation that solves the complete microfluidic system for in-line prediction of the required pressure settings within less than 200 ms. The appropriateness of and benefits from this approach are demonstrated as exemplary for creating multi-component laminar co-flow and the creation of droplets with variable composition. Image-based methods were combined with chemometric approaches for the readout and correlation of the created multi-component flow patterns with the predictions obtained from the solver.
Multi-Temporal Sentinel-2 Data Analysis for Smallholding Forest Cut Control
Authors: López-Amoedo Alberto , Álvarez Xana , Lorenzo Henrique , Rodríguez Juan Luis
Published: July 2021

Land fragmentation and small plots are the main features of the rural environment of Galicia (NW Spain). Smallholding limits land use management, representing a drawback in local forest planning. This study analyzes the potential use of multitemporal Sentinel-2 images to detect and control forest cuts in very small pine and eucalyptus plots located in southern Galicia. The proposed approach is based on the analysis of Sentinel-2 NDVI time series in 4231 plots smaller than 3 ha (average 0.46 ha). The methodology allowed us to detect cuts, allocate cut dates and quantify plot areas due to different cutting cycles in an uneven-aged stand. An accuracy of approximately 95% was achieved when the whole plot was cut, with an 81% accuracy for partial cuts. The main difficulty in detecting and dating cuts was related to cloud cover, which affected the multitemporal analysis. In conclusion, the proposed methodology provides an accurate estimation of cutting date and area, helping to improve the monitoring system in sustainable forest certifications to ensure compliance with forest management plans.
Satellite-Derived Estimation of Grassland Aboveground Biomass in the Three-River Headwaters Region of China during 1982–2018
Authors: Yu Ruiyang , Yao Yunjun , Wang Qiao , Wan Huawei , Xie Zijing , Tang Wenjia , Zhang Ziping , Yang Junming , Shang Ke , Guo Xiaozheng , Bei Xiangyi
Published: July 2021

The long-term estimation of grassland aboveground biomass (AGB) is important for grassland resource management in the Three-River Headwaters Region (TRHR) of China. Due to the lack of reliable grassland AGB datasets since the 1980s, the long-term spatiotemporal variation in grassland AGB in the TRHR remains unclear. In this study, we estimated AGB in the grassland of 209,897 km2 using advanced very high resolution radiometer (AVHRR), MODerate-resolution Imaging Spectroradiometer (MODIS), meteorological, ancillary data during 1982–2018, and 75 AGB ground observations in the growth period of 2009 in the TRHR. To enhance the spatial representativeness of ground observations, we firstly upscaled the grassland AGB using a gradient boosting regression tree (GBRT) model from ground observations to a 1 km spatial resolution via MODIS normalized difference vegetation index (NDVI), meteorological and ancillary data, and the model produced validation results with a coefficient of determination (R2) equal to 0.76, a relative mean square error (RMSE) equal to 88.8 g C m−2, and a bias equal to −1.6 g C m−2 between the ground-observed and MODIS-derived upscaled AGB. Then, we upscaled grassland AGB using the same model from a 1 km to 5 km spatial resolution via AVHRR NDVI and the same data as previously mentioned with the validation accuracy (R2 = 0.74, RMSE = 57.8 g C m−2, and bias = −0.1 g C m−2) between the MODIS-derived reference and AVHRR-derived upscaled AGB. The annual trend of grassland AGB in the TRHR increased by 0.37 g C m−2 (p < 0.05) on average per year during 1982–2018, which was mainly caused by vegetation greening and increased precipitation. This study provided reliable long-term (1982–2018) grassland AGB datasets to monitor the spatiotemporal variation in grassland AGB in the TRHR.
Modified Interpolation Method of Multi-Reference Station Tropospheric Delay Considering the Influence of Height Difference
Authors: Pu Yakun , Song Min , Yuan Yunbin
Published: July 2021

In network real-time kinematic (NRTK) positioning, atmospheric delay information is critical for generating virtual observations at a virtual reference station (VRS). The traditional linear interpolation method (LIM) is widely used to obtain the atmospheric delay correction. However, even though the conventional LIM is robust in the horizontal direction of the atmospheric error, it ignores the influence of the vertical direction, especially for the tropospheric error. If the height difference between the reference stations and the rover is large and, subsequently, tropospheric error and height are strongly correlated, the performance of the traditional method is degraded for tropospheric delay interpolation at the VRS. Therefore, considering the height difference between the reference stations and the rover, a modified linear interpolation method (MLIM) is proposed to be applied to a conventional single Delaunay triangulated network (DTN). The systematic error of the double-differenced (DD) tropospheric delay in the vertical direction is corrected first. The LIM method is then applied to interpolate the DD tropospheric delay at the VRS. In order to verify the performance of the proposed method, we used two datasets from the American NOAA continuously operating reference stations (CORS) network with significant height differences for experiments and analysis. Results show that the DD tropospheric delay interpolation accuracy obtained by the modified method is improved by 84.1% and 69.6% on average in the two experiments compared to the conventional method. This improvement is significant, especially for low elevation satellites. In rover positioning analysis, the traditional LIM has a noticeable systematic deviation in the up component. Compared to the conventional method, the positioning accuracy of the MLIM method is improved in the horizontal and vertical directions, especially in the up component. The accuracy of the up component is reduced from tens of centimeters to a few centimeters and demonstrates better positioning stability.
Parent and PHY Selection in Slot Bonding IEEE 802.15.4e TSCH Networks
Authors: Daneels Glenn , Van Leemput Dries , Delgado Carmen , De Poorter Eli , Latré Steven , Famaey Jeroen
Published: July 2021

While IEEE 802.15.4e Time-Slotted Channel Hopping (TSCH) networks should be equipped to deal with the hard wireless challenges of industrial environments, the sensor networks are often still limited by the characteristics of the used physical (PHY) layer. Therefore, the TSCH community has recently started shifting research efforts to the support of multiple PHY layers, to overcome this limitation. On the one hand, integrating such multi-PHY support implies dealing with the PHY characteristics to fit the resource allocation in the TSCH schedule, and on the other hand, defining policies on how to select the appropriate PHY for each network link. As such, first a heuristic is proposed that is a step towards a distributed PHY and parent selection mechanism for slot bonding multi-PHY TSCH sensor networks. Additionally, a proposal on how this heuristic can be implemented in the IPv6 over the TSCH mode of IEEE 802.15.4e (6TiSCH) protocol stack and its Routing Protocol for Low-power and Lossy network (RPL) layer is also presented. Slot bonding allows the creation of different-sized bonded slots with a duration adapted to the data rate of each chosen PHY. Afterwards, a TSCH slot bonding implementation is proposed in the latest version of the Contiki-NG Industrial Internet of Things (IIoT) operating system. Subsequently, via extensive simulation results, and by deploying the slot bonding implementation on a real sensor node testbed, it is shown that the computationally efficient parent and PHY selection mechanism approximates the packet delivery ratio (PDR) results of a near-optimal, but computationally complex, centralized scheduler.
The Chemistry and Pharmacology of Fungal Genus Periconia: A Review
Authors: Azhari Azmi , Supratman Unang
Published: July 2021

Periconia is filamentous fungi belonging to the Periconiaceae family, and over the last 50 years, the genus has shown interest in natural product exploration for pharmacological purposes. Therefore, this study aims to analyze the different species of Periconia containing natural products such as terpenoids, polyketides, cytochalasan, macrosphelides, cyclopentenes, aromatic compounds, and carbohydrates carbasugar derivates. The isolated compound of this kind, which was reported in 1969, consisted of polyketide derivatives and their structures and was determined by chemical reaction and spectroscopic methods. After some years, 77 compounds isolated from endophytic fungus Periconia were associated with eight plant species, 28 compounds from sea hare Aplysia kurodai, and ten from endolichenic fungi Parmelia sp. The potent pharmacological agents from this genus are periconicin A, which acts as an antimicrobial, pericochlorosin B as an anti-human immunodeficiency virus (HIV), peribysin D, and pericosine A as cytotoxic agents, and periconianone A as an anti-inflammatory agent. Furthermore, information about taxol and piperine from Periconia producing species was also provided. Therefore, this study supports discovering new drugs produced by the Periconia species and compares them for future drug development.
A Rapid Analytical Approach for Monitoring Pharmaceuticals in Hospital Wastewater—A DPX-Based Procedure with Environmentally-Friendly Extraction Phase Coupled to High Performance Liquid Chromatography–Diode Array/Fluorescence Detectors
Authors: Meneghini Vanessa , Corazza Gabriela , Magosso Hérica A. , Merib Josias , Carasek Eduardo
Published: July 2021

In this study, a novel analytical methodology based on disposable pipette extraction (DPX) was developed using an alternative extraction phase for the extraction/determination of six pharmaceutical compounds, including carbamazepine, diclofenac, naproxen, fluoxetine, losartan and 17α-ethinylestradiol, in samples of hospital wastewater by high-performance liquid chromatography coupled to diode array and fluorescence detectors. The performance of three extraction phases was examined, including 3-n-propyl (3-methylpyridinium) silsesquioxane chloride (Si3Py+Cl−), the conductive polymer polypyrrole (PPy), and polypyrrole modified with cetyltrimethylammonium bromide (PPy.CTAB). The optimization of the experimental parameters was performed through univariate and multivariate approaches. The optimized condition was obtained with the use of 20 mg of Si3Py+Cl− as extraction phase; six extraction cycles with 700 μL of sample in each cycle and 15 s of extraction time; three desorption cycles with 100 μL of ACN (same aliquot) and 15 s of desorption time; and sample pH adjusted at 3.5 and addition of 15% (w/v) of NaCl in the sample. The methodology proposed exhibited environmentally-friendly aspects with a significantly reduced volume of organic solvent (only 100 µL) and a small amount of extraction phase (20 mg). In addition, the extraction phase employed exhibits a simple synthetic procedure, low cost, and high stability in organic solvent. Moreover, the method developed exhibits high throughput (extraction time of 6.5 min per sample), and robustness. The analytical figures of merit were obtained using hospital wastewater, and the values were very satisfactory. The correlation coefficients were higher than 0.9710. LODs and LOQs ranged from 0.030 µg L−1 to 1.510 µg L−1 and 0.10 µg L−1 to 5.00 µg L−1, respectively. Relative recoveries varied from 80 to 127%, and intra-day (n = 3) and inter-day (n = 9) precision was lower than 19%.
The Psychological and Social Impacts of Curfew during the COVID-19 Outbreak in Kuwait: A Cross-Sectional Study
Authors: Salman Ahmad , Al-Ghadban Fatima , Sigodo Kennedy Ouma , Taher Ayyoub K. , Chun Sungsoo
Published: July 2021

The Coronavirus (COVID-19) pandemic in Kuwait led to a nationwide curfew between 22 March and August 2020. The purpose of this study was to evaluate the impact of the COVID-19 curfew during the pandemic on Kuwaiti citizens and residents. A cross-sectional survey was used to collect data from Kuwaiti residents over the age of 21 through an online questionnaire shared via social media, including WhatsApp and Facebook. Data collection occurred between 18 June and 15 July 2020. Data from 679 respondents (57.9% females and 42.1% males; 67.7% Kuwaiti nationals and 32.3% non-Kuwaiti nationals) were analyzed. Symptoms of depression were reported among 59.8% of females and 51.0% of males, and extremely severe depression among 20.4% of females and 13.6% of males. Approximately 42.0% of females and 37.8% of males were under psychological distress, with 15.1% of females and 9.1% of males experiencing severe or extremely severe psychological distress. Over a third of females (34.9%) reported experiencing tensions or violent behaviors from family members, and 22.1% reported verbal or physical abuse. Among males, 26.4% reported experiencing tensions or violent behaviors, and 12.2% reported verbal or physical abuse. Extremely severe depression was associated with being female (2.00 times), aged 21–29 (4.56 times), experiencing tensions or violent behaviors from family members (4.56 times), being physically inactive (1.64 times), smoking cigarettes (3.02 times), and having poor or very poor quality of sleep (1.75 times). Severe or extremely severe psychological distress was associated with being female (3.09 times), aged 21–49 (3.68 times), having ill-health conditions or diseases (1.83 times), experiencing tension or violent behaviors from family members (3.56 times), smoking cigarettes (3.06 times), and having poor or very poor quality of sleep (2.20 times). Findings indicate that people living in Kuwait experienced negative psychological impacts, such as depression and psychological distress, attributable to the pandemic-related restrictions. Unpartnered females aged 21–49 are more mentally vulnerable than partnered males over the age of 50. Findings support an urgent need for targeted interventions to improve health behaviors and social support, including coping mechanisms specific to COVID-19 related stress, family counseling systems, and the provision of accessible and acceptable services using telehealth.
Honeybee and Plant Products as Natural Antimicrobials in Enhancement of Poultry Health and Production
Authors: Lika Erinda , Kostić Marija , Vještica Sunčica , Milojević Ivan , Puvača Nikola
Published: July 2021

The quality and safety attributes of poultry products have attracted increasing widespread attention and interest from scholarly groups and the general population. As natural and safe alternatives to synthetic and artificial chemical drugs (e.g., antibiotics), botanical products are recently being used in poultry farms more than 60% of the time for producing organic products. Medicinal plants, and honeybee products, are natural substances, and they were added to poultry diets in a small amount (between 1% and 3%) as a source of nutrition and to provide health benefits for poultry. In addition, they have several biological functions in the poultry body and may help to enhance their welfare. These supplements can increase the bodyweight of broilers and the egg production of laying hens by approximately 7% and 10% and enhance meat and egg quality by more than 25%. Moreover, they can improve rooster semen quality by an average of 20%. Previous research on the main biological activities performed by biotics has shown that most research only concentrated on the notion of using botanical products as growth promoters, anti-inflammatory, and antibacterial agents. In the current review, the critical effects and functions of bee products and botanicals are explored as natural and safe alternative feed additives in poultry production, such as antioxidants, sexual-stimulants, immuno-stimulants, and for producing healthy products.
Impacts, Systemic Risk and National Response Measures Concerning COVID-19—The Island Case Studies of Iceland and Greenland
Authors: Cook David , Jóhannsdóttir Lára
Published: July 2021

The Arctic is a remote region that has become increasingly globalized, yet it remains extremely vulnerable to many risks. The COVID-19 pandemic presented new challenges to the region. Using the search, appraisal, synthesis and analysis (SALSA) approach to conduct a meta-synthesis of the academic and grey literature on the impacts of the pandemic, an assessment is conducted of the types of risks that have been presented by the COVID-19 pandemic, the scales, and the national response strategies for mitigating the risks. Two case studies are explored: Iceland and Greenland, island nations that exemplify the extremes of the Arctic and reliance on tourism, a sector that was nearly entirely suspended by the pandemic. An evaluative matrix is employed which combines five different scales of risk—nano, micro, meso, macro and cosmic—with a sustainability categorization of impacts. The risks of the pandemic cut across the respective scale and categories, with the potential for macro-scale events (systemic risk) to unfold linked to economic spillover effects driven by the curtailment of tourism and various supply chain delays. Both Iceland and Greenland have exemplified risk mitigation strategies that prioritize health over wealth, very strictly in the case of the latter. Strict border controls and domestic restrictions have enabled Iceland and Greenland to have much lower case and death numbers than most nations. In addition, Iceland has led the way, globally, in terms of testing and accumulating scientific knowledge through genetic sequencing of the virus. The academic contribution of the paper concerns its broadening of understanding concerning systemic risk, which extends beyond financial implications to includes sustainability dimensions. For policymakers and practitioners, the paper highlights successful risk mitigation and science-based measures that will be useful for any nation tackling a future pandemic, regardless of whether they are island states, Arctic nations or another country.