bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics and outcomes amongst stroke patients in symptomatic and asymptomatic COVID-19: a systematic review
Authors: Pamungkas Dimas Bathoro Bagus , Pintoko Kalanjati Viskasari , Abdurrachman
Published: May 2024

Link of Video Abstract: https://www.youtube.com/watch?v=1zdsWuZBGgcIntroduction: Stroke has been reported in symptomatic and asymptomatic COVID-19 patients with undetermined characteristics and outcomes. Stroke is one of the non-respiratory pathology reported amongst COVID-19 patients with various manifestations. We elucidated the characteristics and outcomes in symptomatic and asymptomatic COVID-19 patients.
Methods: A systematic review was performed from 01/01/2020 to 16/03/2022 in EBSCOhost, Google Scholar, Pubmed, Scopus, and Web of Science with search terms, i.e., COVID-19, Coronavirus, SARS-CoV-2, Coronavirus Disease-19, stroke, CVA (cerebrovascular accident), brain MRI, head MRI, magnetic resonance imaging, antithrombotic agent, thrombectomy, using the PRISMA guidelines. Inclusion criteria include case reports or series of adult stroke patients with confirmed COVID-19 written in English; other articles were excluded.
Results: From 239 articles screened initially, 23 articles were included. Twenty-five patients are classified as symptomatic (n=17) and asymptomatic (n=8). In symptomatic patients, motor and sensory abnormalities with loss of consciousness and alteration of memory were detected. Eight symptomatic patients had elevated D-dimer, and 2 had elevated fibrinogen levels. Complete resolution was found 6 times higher in symptomatic vs. asymptomatic cases, while the mortality rate was lower in symptomatic compared to asymptomatic patients.
Conclusions: Stroke in symptomatic patients occurred more frequently than the asymptomatic and was observed pre- or post-diagnosis of COVID-19; thus could be the comorbidity and/ or complication.
The comparison of GNRI versus HALP score as an outcome predictor in geriatric rectal cancer patients
Authors: Fuadhi Singgih Annas , Handaya Adeodatus Yuda , Setyawan Nurcahya , Barmawi Agus , Sofii Imam
Published: May 2024

Link of Video Abstract: https://youtu.be/mBRqppCkDcM
 
Background: Colorectal cancer is the third most common cancer worldwide. Along with the development of science and technology, life expectancy has also increased, but the number of geriatric patients with colorectal cancer and undergoing surgery has also increased. Geriatric patients have many factors that can affect the patient's prognosis. Thus, a screening tool to predict the outcome of rectal cancer geriatric is needed. 
Methods: An observational analytic study with a retrospective cohort study design. This research was conducted by taking medical record data and laboratory results of patients with rectal cancer treated at Dr. Sardjito General Hospital, Yogyakarta, Indonesia. Data were analyzed using SPSS version 20.0 for Windows.
Results: Patients with malnutrition, decreased hemoglobin levels, albumin, and increased platelets affect mortality and postoperative complications of rectal cancer patients. There was a significant association between the Geriatric Nutritional Risk Index (GNRI) and preoperative Hemoglobin, Albumin, Lymphocyte, and Platelet (HALP) scores on mortality and postoperative complications in geriatric patients with rectal cancer (p<0.05). GNRI associated with length of stay in geriatric patients with rectal cancer. However, there is no significant relationship between Hemoglobin, Albumin, Lymphocyte, and Platelet (HALP) preoperative scores on length of stay in geriatric patients with rectal cancer (p>0.05).
Conclusion: Both screening predictors of rectal cancer outcome, namely GNRI and HALP, have a significant relationship with patient mortality and complications.
Telerehabilitation of spinal cord injury in limited resources setting during COVID-19 pandemic: A case report
Authors: Azharuddin  , Novera Diaz
Published: May 2024

Link of Video Abstract: https://youtu.be/f6IaLnu3sz8
 
Introduction: Spinal cord injury (SCI) rehabilitation is a resource-intensive and time-consuming process and this is problem in particular for patients from a rural, remote areas, or low socioeconomic backgrounds. This problem is further exacerbated by social restriction policies in place due to coronavirus disease 2019 (COVID-19). This case report aimed to describe how telerehabilitation could overcome these challenges
Case presentation: A 20-year-old female involved in a motor vehicle accident. Preoperative magnetic resonance imaging (MRI) indicated  a complete burst fracture of the L1 vertebral body. The patient experiences paraplegia and loss of autonomic control. Long spine fusion was performed. After being discharged, the patient could only visit the hospital once one-month post-surgery due to personal financial reasons and COVID-19-related social distancing policy. Telerehabilitation was used for the remaining follow-ups and rehabilitation programs were monitored through instant messaging platform between the patient, the operating surgeon, and the rehabilitation specialist. Telerehabilitation using instant messaging was an affordable and practical platform of communication for patient self-reporting and monitoring of progress. With intensive telerehabilitation program, the patient’s functional abilities recovered from Frankel B to D after 6 months of following the domiciliary telerehabilitation plan.
Conclusion: We report a case of satisfactory functional outcome of SCI rehabilitation following the use of instant messaging to assist the rehabilitation. This highlights that instant messaging may be utilized to guide the rehabilitation process with the consideration of other factors such as the patient's engagement and the healthcare provider’s commitment. We recommend for further refinements in the adoption and use of telerehabilitation for  orthopaedic and traumatology cases that need long-term rehabilitation.
Correlation of serum D-dimer level and the event microvessel cardiac thrombosis observed on core biopsy in post mortem COVID-19 Patients in Dr. Soetomo Hospital Surabaya, Indonesia
Authors: Lefi Achmad , Rinjani Lalu Galih Pratama , Alsagaff Muhammad Yusuf , Budiarto Raden Mohammad , Wiratama Priangga Adi , Kusumastuti Etty Hary , Marhana Isnin Anang , Rosyid Alfian Nur , Wahyu Dwi , Semedi Bambang Pujo , Nugraha Ricardo Adrian , Intan Ryan Ernast , Ramadhan Muhammad , Caesarina Ika , Fadila Asiyah Nurul , Tiyantara Muhammad Surya , Nugroho Gilang Muhammad Setyo , Maimunah Ummi , Suyanto Edi , Anggoro Adhitri , Yandi I Komang Rusgi , Lilihata Jilieanastasia Godrace
Published: May 2024

Link of Video Abstract: https://youtu.be/ivpwfj9PxWQ
 
Background: Microvascular coronary thrombosis is an emerging risk factor that worsens the prognosis of COVID-19 patients. This study aims to show for the first time a descriptive of histopathologic findings from post-mortem COVID-19 patients and to analyze whether D-Dimer serum level, a marker of hyper-coagulopathy, correlates with coronary microvascular thrombosis from the cardiac core biopsy.
Method: This was an observational analytic study with a retrospective cohort design from July-December 2020. Cardiac core biopsy was taken from patients who died while treated at the isolation ICU at Dr. Soetomo due to severe COVID-19. The samples were taken in 1-hour post-mortem and then analyzed histopathologically with Hematoxylin-eosin staining under a light microscope to evaluate the presence of coronary microvascular thrombosis and other pathological findings from the cardiac biopsy. Clinical information and D-Dimer levels from medical records and analyzed for coronary microvascular thrombosis using Man-Whitney and C-statistic analysis using SPSS 22 software.
Result: There were 39 samples of post-mortem patients in this study. The majority were men (71.8%), with a mean age of 48.9 years old. Focal microvessel coronary thrombosis was found in 28%). The median D-Dimer level was increased from the average baseline (3460 mg/dl). However, there was no significant difference in D-dimer levels between focal microvessel coronary thrombosis incidents (p-value 0.827, C statistic AUC 0.523). The lack of focal necrosis in the surrounding tissue suggests that the thrombosis resulted from proximal embolization to distal capillary coronary, which already happened before, rather than the primary in-situ process in microvascular, hence may explain why D-Dimer was not correlated with the finding of coronary microvascular thrombosis in this study.
Conclusion: D-dimer serum levels were not associated with focal microvessel thrombi in post-mortem COVID-19 patients. This result supports previous studies that showed D-Dimer was not specific to detect thrombosis in microvascular.
The impact of social media marketing efforts on visit intention: a case study in Flodova Clinic, Dili, Timor Leste
Authors: Gomes Florindo Cardoso , Antonio Ferdi , Laot Perpetua Ana Mery Estela , Gomes Eva Estrelita Cardoso
Published: May 2024

Link of Video Abstract: https://youtu.be/iDWJ07s4En8
 
Background: The utilization of social media in digital marketing offers various benefits, notably improved accessibility and the ability to disseminate information widely among diverse populations. Notably, social media has emerged as the leading marketing strategy to generate interest among people visiting healthcare facilities in Timor Leste. This study examines the impact of Social Media Marketing Efforts (SMME) on visit intentions through Flodova Clinic's Facebook page in Timor Leste. The mediating factors of brand image and online trust are investigated while controlling for health threats and gender.
Methods: The study collects data from visitors and Facebook followers of Flodova Clinic. A cross-sectional data collection was done through an online survey. The analysis includes 184 samples that meet the inclusion criteria, employing Partial Least Squares Structural Equation Modeling (PLS-SEM).
Results: The findings demonstrate a significant influence of SMME on visit intentions, indicating its efficacy in attracting potential visitors. However, no direct or mediating effects are observed on brand image and online trust. The study identifies the most influential domains of SMME, with electronic word of mouth (EWOM) having the most substantial impact, followed by entertainment and interaction. Additionally, health threats and gender are found to affect visit intentions significantly. Health threats positively influence patients' intent to visit, while females show comparatively lower interest in visiting the Flodova clinic.
Conclusions: These results have managerial implications for developing SMME strategies in marketing health clinics, mainly through social media platforms like Facebook. The study underscores the importance of leveraging electronic word of mouth, entertainment, and interactive elements to maximize the effectiveness of SMME strategies. Additionally, it emphasizes the need for tailored approaches considering health threats and gender differences to optimize visit intentions and enhance engagement.
Mother's attitude to breastfeeding and its effect on oxytocin levels in mothers: a review article
Authors: Fikri Lalu Hamzi , Suryani As’ad  , Mochammad Hatta  , Saidah Syamsuddin  , Hanung Prasetya  , Martira Maddeppungeng  , Aminuddin  , Andi Alfian Zainuddin
Published: May 2024

Link of Video Abstract: https://youtu.be/Rg5QfPqxtqABackground. Breast emptying causes more milk to be released, which stimulates the production of breast milk. One of the crucial hormones that will rise with breastfeeding is oxytocin. This literature study aims to evaluate the mothers' attitudes toward giving breastfeeding and how it relates to the mother's oxytocin levels is reviewed in this study.
Methods. We used the phrases "mother's attitude," "breastfeeding," and "oxytocin level" in several databases, including PubMed (NIH), Scopus, EMBASE, and Google Scholar, as keywords for literature searching. Also manually searched were pertinent reference databases. This review article integrated any previously recognized relevant publications from the database, explained the findings in a narrative format, and offered a thorough evaluation and analysis of the literature findings.
Results. According to this study, oxytocin levels are linked to many mothers' attitudes toward nursing, most favorable. Several studies indicate that the oxytocin levels increase high empathy, reduce stress, reduce cortisol hormone, typical of bonding, and mother will feel confident.
Conclusion. The mother's mindset and the increase in oxytocin are related to breastfeeding.
The effect of Mozart's classical music on blood pressure in Wistar white rats (Rattus norvegicus)
Authors: Tandirerung Rivans Jackson , Irwanto  , Krisna Anak Agung Ngurah
Published: May 2024

Introduction: Classical music has beneficial effects on heart rate variability and also reduces one's stress levels due to parasympathetic dominance. Mozart's classical music was shown to provide subsequent short-term enhancement of spatial-temporal reasoning ability. This research aimed to study the effect of Mozart's classical music on blood pressure.
Methods: The design of this study is true experimental with a post-test-only design with a control group.
Thirty Wistar white rats were randomized by simple random sampling method into the control group and experimental group receiving Mozart’s classical music for 90 minutes, twice daily for 1, 3, and 4 weeks. SBP, DBP, and MAP were measured and analyzed using descriptive analysis, Shapiro Wilk, Mann-Whitney U, and Wilcoxon Sign Rank test.
Results: DBP and MAP of the experimental group week 1 and 3 were significantly different from the control group. There were significant differences between SBP, DBP, and MAP of experimental group week 1 from week 3 (p=0.00, p=0.00, p=0.00 respectively) and week 1 from week 4 (p=0.00, p=0.00, p=0.00).
Conclusion: Listening to Mozart's classical music has a direct effect on blood pressure.
The correlation between serum matrix metalloproteinase-9 levels and the severity of coronary artery stenosis in patients with acute coronary syndrome
Authors: Rasaki Rico , Heriansyah Teuku , Ridwan Muhammad , Novita  , Munirwan Haris
Published: May 2024

Link of Video Abstract: https://www.youtube.com/watch?v=mzVt00YaibkIntroduction: Atherosclerosis causes coronary artery stenosis in a lot of patients with acute coronary syndrome (ACS). By controlling platelet activity and destroying the extracellular matrix, a group of enzymes known as matrix metalloproteinases (MMPs) contribute to the plaque rupture process that causes ACS. The study aimed to determine whether there was any correlation between the amount of serum MMP-9 and the severity of coronary artery stenosis in ACS patients.
Methods: Cross-sectional analytical observational design was employed in this investigation. The research population consisted of ACS patients at the Dr. Zainoel Abidin General Hospital in Banda Aceh, Indonesia. Patients who were included as samples had to satisfy the inclusion and exclusion requirements. This study used analysis of variance (ANOVA) as a bivariate analysis to examine the connection between blood MMP-9 levels and the severity of coronary artery stenosis. The threshold for statistical significance was a p-value≤0.05.
Results: This research comprised 40 patients with ACS, of which 14 had STEMI, 11 had NSTEMI, and 15 had unstable angina pectoris (UAP). Eleven individuals had mild coronary artery stenosis, eleven had moderate stenosis, and eighteen had severe stenosis, according to an analysis of the Gensini score. Bivariate analysis revealed no significant relationship between blood MMP-9 levels and the degree of coronary artery stenosis in ACS patients (p-value = 0.434).
Conclusions: In patients with ACS at the Dr. Zainoel Abidin General Hospital in Banda Aceh, Indonesia, there was no statistically significant association between serum MMP-9 levels and the degree of coronary artery stenosis, according to this study.
Predictor of Mortality in Complicated Intraabdominal Infection Patients at Dr. Soetomo General Hospital Surabaya
Authors: Kurniawan Erwin , Wibowo Marjono Dwi , Danardono Edwin
Published: May 2024

Link of Video Abstract: https://youtu.be/g_ys7cQCuDYBackground: Complicated intraabdominal infection (cIAI) is a surgical emergency reported to be a major contributor to non-traumatic mortality worldwide. Identifying the mortality risk before any operation is important in guiding clinical decision-making and informed patient consent about the risk of complicated intraabdominal infection (cIAI). The study aimed to develop a novel scoring system for predicting postoperative mortality in cIAI.
Methods: Data were collected retrospectively from all consecutive patients 396 patients met the inclusion criteria after excluding missing data undergoing cIAI surgery in Dr. Soetomo Hospital 2020 - 2022. Multivariate logistic regression analysis was performed to correlate the explanatory variable postoperative mortality. Data were analyzed using SPSS version 20.0 for Windows and MedCalc.
Results: Postoperative mortality rate cIAI was  32,2% (128 of 396), and variables identified as the strongest predictors of postoperative mortality were age > 60 y.o (OR 3,196), systolic blood pressure < 100 mmHg (OR 5,894), thrombocyte < 100.000 /uL (OR 5,593), albumin ≤ 2,9 g/dL (OR 6,764), total bilirubin > 1,8 mg/dL (OR 2,180), creatinine serum ≥ 1,58 mg/dL (OR 4,290), cancer comorbidity (OR 3,578), and appendix perforation as negative predictor (OR 5,853). These parameters were included in the prediction model of the novel simplified Airlangga Scoring System.
Conclusion: Despite the relatively low number of risk factors, the Airlangga score has been shown as a good predictor of postoperative mortality after cIAI. External validation is required in hospitals different from those in which the novel scoring system was developed.
Effect of human granulosa cells vitrification on the expression of oocyte secreted factors
Authors: Batara Sirait  , Budi Wiweko  , R. Muharram  , Ahmad Aulia Jusuf  , Ichramsjah A. Rachman  , Arief Boediono
Published: May 2024

Background: Oocyte vitrification is a helpful fertility preservation technique for women at risk of losing their ovarian functions. Meanwhile, its application extends with the emerging trend of delayed childbearing, postponed marriage, and further diverse ethical, medical, legal, and social implications. Whereas its effect on oocytes has been widely elucidated, the potential impact of this procedure on the biological functions of granulosa cells remains poorly understood. The present study aimed to assess vitrification’s effect on the granulosa cells (GCs).
Methods: 35 women with polycystic ovary syndromes who underwent in vitro fertilization (IVF) in Morula IVF Jakarta Clinic were recruited. The expression of prominent oocytes secreted factors (OSFs), including GDF-9 and BMP-15, were measured at messenger RNA (mRNA) levels. GCs from mature and immature oocytes were collected and calculated separately. Relative expression of GDF-9 and BMP-15 was quantified by a real-time quantitative PCR (RT-qPCR). All of the data was analyzed by using SPSS.  Bivariate analysis used the Mann-Whitney test at a 95% confidence level. A significant p-value was ≤ 0.05.
Results: The mean age of subjects was 32,39 ± 3,33 years old, with the mean duration of infertility being six years. The mean BMI, anti-Mullerian hormone (AMH) level, and antral follicle count (AFC) were 24.04 kg/m2, 3.43 ng/mL, and 15 follicles, respectively. The basal FSH, LH, estradiol, and progesterone were at the normal level. The expression of GDF-9 decreased significantly (0.5-fold, p < 0.01) in the immature vitrified GC group but was not in the mature-vitrified GC group. Meanwhile, the expression of BMP-15 was stable in all examined groups (P>0.05).
Conclusion: Our results suggest that vitrification may alter oocyte maturation, as demonstrated by reduced GDF-9 expression in the immature vitrified GC group.
Simvastatin: a new therapeutic strategy for Chronic Kidney Disease (CKD)
Authors: Cahyawati Putu Nita , I Nyoman Mantik Astawa  , Bagus Komang Satriyasa  , I Made Bakta
Published: May 2024

Link of Video: https://youtu.be/KtNkkeJmbh4Background: Chronic kidney disease (CKD) is a progressive disease whose prevalence increases annually. Studies in recent years report that statins (hydroxy-3-nethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors) affect preventing the progression of kidney damage, in addition to their ability to prevent cardiovascular risk in patients with CKD. This study aims to determine the effect of statins on kidney function and renal fibrosis in chronic kidney disease.
Methods: This research is pure experimental (randomized post-test only control group design). The research sample consisted of 20 white mice (Mus musculus L.), Swiss line, aged 3-4 months, and weight 30-40 grams. Samples were randomly grouped into four treatment groups: control (K, n = 5), treatment with simvastatin at a dose of 5.2 mg/kgBW (P1), treatment with simvastatin at a dose of 10.4 mg/kgBW (P2), and treatment with simvastatin at a dose of 20.8 mg/kgBW (P3). Interstitial fibrosis was assessed using Picrosirus Red staining. Data analysis used One-Way ANOVA and Tukey's post hoc tests with a significance value of p<0.05 in SPSS version 25.0 for Windows.
Results: The results showed that the control group (K) was the group with the highest serum creatinine level (1.07 ± 0.43) compared to the other treatment groups (p <0.05). Administration of simvastatin at doses of 5.2 mg/kgBW, 10.4 mg/kgBW, and 20.8 mg/kg BW in CKD mice resulted in lower interstitial fibrosis than controls. There was a significant difference between renal tissue fibrosis in the simvastatin group at a dose of 5.2 mg/kgBW (P1) with a dose of 10.4 mg/kgBW (P2) and a dose of 20.8 mg/kgBW (P3).
Conclusion: Simvastatin can improve kidney function and reduce the degree of renal fibrosis in animal models of CKD. Thus, it can be a therapeutic strategy for CKD.
Effect of Asiatic acid and dexamethasone administration to interleukin (IL)-1 expression and number of mononuclear cells in extraocular muscle post-strabismus surgery
Authors: Intifada  , Indriaswati Luki , Hermawan Dicky
Published: May 2024

Strabismus surgery aims to improve visual function, binocular vision, and cosmetics by fixing the position of the eyeball. However, inflammation after surgery can lead to fibrous tissue formation. Dexamethasone is routinely used as an anti-inflammatory but has unwanted side effects. On the other hand, Asiatic acid, the most important saponin constituent of Centella asiatica, has anti-inflammatory and anti-fibrous properties. It has been widely used as a traditional medicine plant in China, India, and Indonesia. The findings show that Asiatic acid has been shown to affect wound healing after strabismus surgery positively. Asiatic acid inhibits inflammation, increases cell proliferation, and inhibits fibrosis. It promotes wound healing by increasing the strength of the incision wound, epithelialization, keratinization, and angiogenesis. Overall, using natural compounds like Asiatic acid in wound healing is promising and should be explored further. It can be a safer and more effective alternative to dexamethasone, which has known side effects. However, further studies are required to determine the optimal dosage and administration method of Asiatic acid for post-operative strabismus wound healing. The results of this study are expected to be the basis for selecting alternative therapies to reduce inflammation and fibrosis after strabismus surgery.
The black cumin seeds oil with Curcuma xanthorrhiza extract preparation increases IFN-γ expression
Authors: Hidayati Titiek , Sun Sunny , Akrom
Published: May 2024

Introduction: According to empirical evidence, Curcuma xanthorrhiza (CX) and black cumin (BC) have been utilized extensively in Indonesia. The primary components of Curcuma xanthorrhiza and black cumin (CXBC) formulations are BC oil and CX extract. This study aimed to assess the influence of CXBC active compounds (polyphenols, flavonoids, and thymoquinone) on IFN-g expression in HTB-183 cells. This study aimed to determine the levels of vitamins and minerals in CXBC preparation and the potential for immunomodulatory activities.
Methods: We engaged in experimental laboratory research. Observing the expression of interferon-gamma (IFN-γ) was used to evaluate the immunomodulatory activities of the HTB-183 human large lung cancer cell line.
Results: The results demonstrated that the CXBC supplement included vitamins (A, C, and E) and minerals (potassium, calcium). CXBC preparations boosted HTB-183 cell IFN-γ expression.
Conclusion: The study's findings indicate that the CXBC formulation contains a high concentration of vitamins and minerals. CXBC formulations augment IFN-γ expression.
Eucalyptus oil as adjuvant therapy for coronavirus disease 19 (covid-19): a randomized clinical trial
Authors: Santoso Arif , Rasiha  , Afifah Muthiah Nur , Muhammad Munawir , Sultan Andi Rofian , Juni Muhammad Rezky , Prasetia Priady Wira , Andreza  , Idrus Idrianti , Munawirah Andi , Widaningsih Yuyun , Hamid Firdaus , Paturusi Idrus A. , Massi M. Nasrum , Azis Asrul Abdul , Dharmayanti Niluh Putu Indi , Nuradji Harimurti , Nurjanah Diana , Djufry Fadjry , Safitri Evie
Published: May 2024

Link of Video Abstract: https://youtu.be/WzYiDQR2RvA
 
Introduction: The coronavirus disease 19 (COVID-19) vaccination scheme is still being implemented worldwide, but problems with stock availability and other factors are still impeding this endeavor, especially in developing nations like Indonesia. Ethnopharmacology is known to provide a good source of a cheap candidate for anti-inflammation drugs. This study was conducted to determine the effectiveness of affordable adjuvant therapy, Eucalyptus oil, which has been used as a household remedy for respiratory tract infections in Indonesia.
Methods: 52 mild-moderate COVID-19 patients were randomly assigned for a prospective clinical trial. A group of 26 patients was given standard COVID-19 treatment as a control group, while another 26 patients as the treated group were given standard therapy and use Eucalyptus oil as an adjuvant. The clinical sign and symptoms, cytokines interleukin (IL) 6, IL-10, and transforming growth factor-beta (TGF-β) profiles were recorded during the study period.
Result: There was a trend of better improvement of clinical symptoms among patients who received adjuvant treatment than the control group. Altered smell (p = 0.003) and cough (p = 0.641) recovered faster in the treated group. While there was no marked difference in IL-10 and TGF-β levels among both groups, IL-6 level in the treated group was significantly lower and the neutrophil-to-lymphocyte ratio (NLR) was significantly decreased (p < .05) than the control.
Conclusion: Using Eucalyptus oil as an adjuvant of COVID-19 treatment promotes faster recovery of mild-moderate COVID-19 patients, probably via inhibition of the IL-6-mediated inflammation process.
Umbilical cord-derived mesenchymal stem cell for reducing neointimal hyperplasia: a literature review – exploring possible therapeutic use on arteriovenous fistula patient
Authors: Habibie Yopie Afriandi , Emril Dessy Rakhmawati , Azharuddin  , Syahrizal Dedy
Published: May 2024

Link of Video Abstract: https://youtu.be/2uoN5ciMk14
 
Umbilical cord mesenchymal stem cells have been shown to reduce neointimal hyperplasia in animal models. However, current studies about therapeutic use to reduce neointimal hyperplasia mainly utilized bone-marrow and adipose tissue-derived mesenchymal stem cells and lack of in vivo testing from umbilical cord mesenchymal stem cells. Herein, we explore the potential of the therapeutic use of umbilical cord-derived mesenchymal stem cells to reduce neointimal hyperplasia in patients with arteriovenous fistula. Studies were identified from Scopus, Pubmed, and Google Scholar published between 2000 and 2022. The inclusion criteria for the articles were: (1) written in English, (2) focused on the use of mesenchymal stem cells (MSCs) for the treatment of neointimal hyperplasia, and (3) animal-controlled studies. Exosome-derived from mesenchymal stem cell studies were excluded. A total of 9 articles were included. Overall, the available evidence suggests that UC-MSCs may be a promising therapeutic option for reducing neointimal hyperplasia in arteriovenous fistula (AVF) patients. However, more research is needed to confirm these findings and to determine the optimal dosing and administration for their use in clinical practice. Additionally, further studies are required to fully understand the standardization in isolating and characterizing UC-MSCs and their effects on neointimal hyperplasia.
Chiari Malformation (CM) type III surgical management in infant: a case report
Authors: Baktir Yusuf , Suryaningtyas Wihasto , Parenrengi Muhammad Arifin
Published: May 2024

Background: Chiari Malformations (CM) is a collection of hindbrain abnormalities ranging from simple to complete agenesis of the cerebellum. Type III CM (CM III) is the rarest CM, with herniation of the cerebellum and brainstem into a posterior encephalocele and other intracranial anomalies. Reduction of the viable herniated brain, along with providing intracranial space and inadequate closure, often presents as the main problem. This case study aims to evaluate the Chiari malformation type III surgical management in infants.
Case Presentation: A 16-day-old male infant with an intact congenital mass in the occipital, with no neurological deficit. Radiological examination shows a defect in the occipital skull consisting of cystic non-contrast enhancing lesion and parenchymal tissue herniated through the defect.  The cele excision and defect closure were performed, and viable neural tissue was found inside the sac. Blossom flower-like trim expanded the fossa posterior space and put the viable neural tissue inside the intracranial room. The defect was then closed with a dura, periosteal patch, and tension-free two-layered scalp closure. Post-operative follow-up presented a good outcome, and 1-year follow-up showed good recovery, with developmental delay, following developmental rehabilitation.
Conclusion: CM III is rare and has poor outcomes because neurological deficits and respiratory problems usually follow it. Operative management patient with CM III was presented with occipital encephalocele with no post-operative complication. Occipital bone reconstruction using a blossomed-flower-like technique could be considered to create adequate room for viable neural tissue.
Idiopathic giant scrotal calcinosis: a rare case report and literature review
Authors: Wyckmell Octof Ingratoeboen  , Gerhard Reinaldi Situmorang  , Riesye Arisanty  , Madhyra Tri Indraswari  , Ponco Birowo
Published: May 2024

Link of Video Abstract: https://youtu.be/HwWU4JYsovA
 
Background: Scrotal calcinosis is a rare abnormality of external genitalia. To date, the pathogenesis of this con is still debated. However, reconstructive surgical therapy is still the treatment of choice in managing this disease. This case report presents our experience in performing extensive excision followed by reconstruction of scrotal defects in patients diagnosed with scrotal calcinosis.
Case Presentation: A 62-year-old healthy man was referred to our department with multiple nodules on the testicular sac that did not cause symptoms but began to disrupt his quality of life. The diagnosis was made by physical examination, and anatomic pathology examination results from a needle biopsy that had been done before. Extensive excision of the scrotum lesion was followed by defect closure. There were no significant intraoperative or postoperative. The procedure was uneventful, and the reconstruction results showed satisfying results.
Conclusion: Scrotal calcinosis is an uncommon skin disease caused by insoluble calcium salts accumulated in scrotal skin tissue. Although the origin and etiology of scrotal calcinosis are debatable, surgical excision is the preferred treatment with a low recurrence rate and good cosmetic results.
The relationship between AQP1 expression in the subventricular zone and severity of hydrocephalus in Rattus Norvegicus strain Sprague-Dawley rats
Authors: Januarman  , Parenrengi Muhammad Arifin , Suryaningtyas Wihasto , Utomo Budi , Bajamal Abdul Hafid , Subagio Eko Agus
Published: May 2024

Link of Video Abstract: https://youtu.be/pWRyUuqXX7g
 
Background: Hydrocephalus is a condition of dilation of the ventricles caused by disturbances production, distribution, or absorption of cerebrospinal fluid (CSF). One of the integral membrane proteins identified in facilitating water transport across the plasma membrane is aquaporin-1 (AQP1), which is frequently found in the plexus choroid. However, theoretically also transports water to the subventricular zone (SVZ) with an unknown mechanism. AQP1 expression will be raised in SVZ under hydrocephalus conditions. This study aims to evaluate the relationship between the severity of hydrocephalus and AQP1 levels in SVZ.
Methods: This research was conducted in an experimental design using Rattus Norvegicus rats of the Sprague-Dawley strain, which were injected with kaolin to create a hydrocephalus model. The study included 24 rats, divided into four groups of six each: the control group and the hydrocephalus induction group on day 7, day 14, and day 21. AQP1 expression was observed using immunohistochemical staining and counted semi-quantitatively.
Results: The average AQP1 expression increased with observation time in the rat model in each group. The ANOVA test showed a significant difference between the four study groups (p=0.001). The correlation showed a statistically significant difference (p=0.000). The results showed an increased expression of the SVZ with a higher severity of hydrocephalus.
Conclusion: The severity of hydrocephalus and AQP1 levels in SVZ are correlated, with the latter being higher, the more severe the degree of hydrocephalus.
The immediate effects of Porang-processed rice (Amorphophallus muelleri) on triglyceride levels in patients with type 2 diabetes mellitus and dyslipidemia
Authors: Wicaksono Satrio Adi , Palisma Zahrien Assyifa Nur , Nugroho Taufik Eko , Utami Sulistiyati Bayu
Published: May 2024

Link of Video Abstract: https://youtu.be/HSObtemRZ0M
 
Background: Diabetes Mellitus (DM) is a major cause of death and disability worldwide. Diabetes mellitus is related to metabolic disorders in glucose and lipid levels. Porang tubers contain glucomannan, a substance with many health benefits, such as ameliorating blood glucose and lipid profiles, and has been processed as many food products, such as porang-processed rice (PR). This study investigated the immediate effects of PR on triglyceride levels compared to white rice (WR) in patients with diabetes mellitus (DM) and dyslipidemia.
Methods: It was a randomized clinical study with an experimental pre-and post-test control group design held from October to November 2021 in Diponegoro National Hospital, Semarang, Indonesia. A total of 24 study subjects were selected by consecutive sampling. The inclusion criteria were (1) patients diagnosed with diabetes mellitus (DM) or who have been consuming routine oral antidiabetic drugs and (2) have signed the written informed consent. These subjects were divided into 2 groups, i.e., the treatment group (n=12) and the control group (n=12). The treatment group was given a porang rice (PR) diet of 300 grams, 3 times a day, for 5 days. The control group will consume a white rice (WR) diet for 5 days. Triglyceride levels were measured and compared between both groups before and after the treatment. Triglyceride levels were measured using venous blood sampling. All subjects were still consuming routine medications during the study.
Results: There were no differences in participants' baseline demographic and clinical characteristics. At pre-treatment, there was no significant difference in triglyceride levels between the group with PR and WR (201.5 ± 102.29 vs 220.8 ± 92.08; respectively, p=0.564). At post-treatment, there was no significant difference in triglyceride levels between the group with PR and that with WR (200.0 ± 90.67 vs 224.3 ± 88.03; respectively, p=0.513). However, there was a larger decrease of triglyceride levels (Δtriglyceride) in a group with PR compared to that with WR (29.4 ± 22.81 vs 12.6 ± 7.12; p=0.016).
Conclusion: There were no significant differences in the triglyceride levels before and after porang-processed rice (PR) or white rice (WR) in patients with DM and dyslipidemia. However, there was a larger decrease in triglyceride levels (Δtriglyceride) in a group with PR compared to that with WR. If confirmed by further studies, porang-processed rice might have the role of nutraceutical plants in stabilizing lipid profiles, especially triglyceride levels, in patients with DM and dyslipidemia.
The relationship between the cycle threshold value of SARS CoV-2 with neutrophil lymphocyte ratio (NLR), absolute lymphocyte count (ALC), severity degrees, long of treatment, and outcomes COVID-19 patients
Authors: Aina Nurlaila  , Mohamad Isa  , Rahmiati Lao  , Ira Nurrasyidah  , Isa Ansori  , Erna Kusumawardhani
Published: May 2024

Link of Video Abstract: https://youtu.be/uGfZNQrEQqY
 
Background: Coronavirus disease 2019 (COVID-19) has caused serious public health problems since the end of 2019. Viral load is known in COVID-19 infection, which indicates the number of causative agents and is indirectly assessed by the Cycle threshold value (Ct value). Ct value assessment has not been declared an essential parameter in COVID-19 infection. Due to the high number of COVID-19 cases, an additional strategy is needed to help identify patients at high risk of experiencing severe clinical conditions by knowing the relationship between the Ct value of SARS CoV-2 and neutrophil lymphocyte ratio (NLR), absolute lymphocyte count (ALC), severity, length of stay, and outcomes.
Methods: This was an analytic observational study with cross-sectional approach in confirmed COVID-19 patients to investigate the relationship between the Ct value of SARS CoV-2 and NLR, ALC, severity, length of stay, and outcome of COVID-19 patients. There were 337 patients analyzed with chi square test.
Results: The results of this study indicate that the majority of subjects were male (51%), aged <60 years (27%), Ct value ≤29 (70%), had a critical severity (36.5%), length of stay >10 days (64.1%) and survived (74.7%). Inflammatory markers examination shows NLR<3,13 (57.9%) and ALC<1500 (66.2%). The chi-square test shows a significant relationship between Ct value and ALC, degree of severity, and outcome in COVID-19 patients (p <0.05). No association was found between Ct value, NLR, and length of stay (p>0.05).
Conclusion: There is a relationship between Ct value and ALC, degree of severity, and outcome in COVID-19 patients.
Wound healing of gaster perforation with Human Dried Amniotic Membrane (H-DAM) as biomaterial patch viewing from the fibroblasts counts and collagen density
Authors: Ramadhani Astri Taufi , Ariandi Setiawan  , I Gusti Bagus Adria Hariastawa
Published: May 2024

Link of Video Abstract: https://youtu.be/UldBMnzIelE
 
Background: Gastric perforation in neonates is an emergency in neonatology with a mortality rate of up to 75%. Many studies have been carried out to improve tissue healing, including using various surgical techniques and materials, one of which is using H-DAM technology. Therefore, this study aims to evaluate the differences in the number of fibroblasts and collagen density in gastric perforation repair with H-DAM as a biomaterial patch compared to using an omental patch in New Zealand white rabbits.
Methods: A true-experimental study was conducted among 30 samples of rabbits and none of them dropped out. The inclusion criteria for this study are New Zealand White Rabbit male sex, age 6-9 months with a weight of 2-3 kg, healthy and active. The Exclusion criteria for this study are rabbits not fasted for 12 hours. During the 12-hour fasting period, it behaved aggressively, attacked other rabbits, and found surgical site infection. Data were analyzed using SPSS version 25.0 for Windows.
Results: From the examination results, there was a significant difference in gastric healing in terms of examining the number of fibroblasts with the Kruskal-Wallis test (p=0.000. The One-way ANOVA analysis found that the three data groups had no significant difference (p=0.234).
Conclusion: There is an increase in the number of fibroblasts and collagen density in gastric perforation repair with H-DAM as a biological dressing compared to primary repair of gastric perforation using an omental patch in rabbit models so that H-DAM can be the repair technique of choice in gastric perforation in the rabbit.
Survival rate of children acute lymphoblastic leukemia at the end of therapy protocol with minimal residual disease (MRD) in Prof I G.N.G. Ngoerah General Hospital Denpasar
Authors: Sanjaya Dewa Gde Windu , Ketut Ariawati  , Putu Junara Putra  , Karyana I Putu Gede , Eka Gunawijaya  , I Made Gde Dwi Lingga Utama
Published: May 2024

Link of Video Abstract: https://youtu.be/5DFCQHg86rU
 
Background: Acute lymphoblastic leukemia (ALL) is the most common malignancy in children. Although the survival rate of pediatric patients with ALL is currently improving, cases of relapse still occur. Minimal residual disease (MRD) status is a prognostic factor that plays an important role in recurrence of ALL patients who have been through induction therapy. Aim: To determine the survival rate in pediatric patients with ALL based on negative MRD examination is better than positive MRD at the end of therapy.
Method: A retrospective cohort study with inclusion criteria including pediatric patients aged 1 to 18 years who were diagnosed with ALL in the period of 2017-2019 at Sanglah General Hospital, Denpasar. Subject’s recruitment was initiated when the minimum number of subjects was fulfilled.
Result: From 32 research subjects, the mean age was 5.1 years, the majority obtained MRD+ results, the dominant sex was male, the average leukocyte was 28.33 thousand, most of the subjects had no mediastinal masses, and generally experienced remissions. MRD levels were found to be significantly lower in remitting patients than patients who did not achieve remission (p value <0.05). The Kaplan-Meier estimates for event-free survival on MRD status (negative versus positive) were 84.6% and 42.1% (P = 0.030).
Conclusion: There was a significant relation between MRD levels and the survival rate to achieve remission and MRD levels are significantly lower in remitting patients than patients who do not achieve remission.
Relationship of quinolinic acid and serotonin with depression and pain degree in cancer pain patients: a cross-sectional study
Authors: Ningrum Aji Mustika , Airlangga Prananda Surya , Maskoep Wiwiek Indriyani , Putri Herdiani Sulistyo
Published: May 2024

Link of Video Abstract: https://youtu.be/zg5vubHMcDY
 
Introduction: Chronic pain requires a thorough assessment if it persists in patients with cancer. Depressive disorder is one of the psychosocial problems that can occur in cancer patients. Depressive disorders affect up to 34.4% of patients with cancer in Indonesia, causing increased morbidity and complicating the management of patients. Quinolinic acid (QUIN) and serotonin (5-HT) are chemicals that can affect pain perception and depressive disorders. This study aims to analyze the relationship between quinolinic acid and serotonin levels with depression and pain degree in cancer pain patients.
Methods: A cross-sectional study design was conducted at the Palliative Polyclinic of Dr. Soetomo Surabaya General Hospital, Indonesia. Patients were collected with purposive sampling. The patient's quinolinic acid and serotonin plasma were collected through blood samples and assessed using enzyme-linked immunosorbent assay (ELISA) method. Spearman's comparison test determined the correlation between quinolinic acid and serotonin levels with depression and pain degree.
Results: A total of 76 cancer patients were included in this study. The cancer types reported were pelvic cancer (23 patients, 30.3%), head and neck cancer (21 patients, 27.6%), abdominal cancer (ten patients 13.2%), breast cancer (seven patients, 9.2%), lung cancer (five patients, 6.6%), skin cancer (three patients, 3.9%), and other types of cancers (seven patients, 9.2%). As many as 24 patients (31.6%) had mild depression and only three patients (3.9%) experienced major depression. Mild pain was reported by 47 patients (61.8%) and 11 patients (14.5%) suffered from severe pain. Spearman's comparison test was conducted on quinolinic acid and serotonin levels against depression degree and pain degree, resulting in a weak correlation.
Conclusion: Quinolinic acid and serotonin levels are related to depression and pain degree in cancer pain patients (p<0.05), though with a weak correlation. However, further study is needed to confirm the findings of this study.
Multiple brain abscesses with tetralogy of fallot (TOF): a case report
Authors: Mikaningtyas Lefi , Sugianto Paulus , Ardhi Mohammad Saiful , Tripriyanggara Ardhi , Nafiah Risqon
Published: May 2024

Introduction: Patients with multiple brain abscesses and a secondary diagnosis of tetralogy of fallot (TOF) rarely occur fatal complications. Brain abscess is a very uncommon but potentially fatal infection of the brain parenchyma that can affect 5%-18.7% of the CHD population. The degree of right-to-left shunt is responsible for brain tissue hypoxia, which can lead to cerebral infarction, which is a precursor to cerebral abscess. Therefore, this study aimed to report on a patient with several brain abscesses and TOF.
Case description: A 30-year-old cyanotic male with a chief complaint of gradually decreased consciousness since 2 weeks ago without a history of vomiting. The patient has a clubbing finger. The patient has a TOF history without a prior infection, such as toothache, ear discharge, paranasal infection, cough, or flu. Physical examination revealed the Glasgow Coma Scale of E4VxM6 (global aphasia), right hemiparesis, increased physiological reflexes and positive pathological reflexes in the right of the body. Laboratory results showed slight leucocytosis and hyponatremia and elevated CRP. Head MRI spectroscopy was performed 3 days later, revealing multiple brain abscesses. The patient was given therapy antibiotics, namely ceftriaxone injection and metronidazole injection intravenously.
Conclusion: A brain abscess should be suspected in patients with TOF who arrive with a fever and an acute neurological condition. Besides antibiotics, patients should be encouraged to have corrective surgery as soon as possible to avoid future deadly consequences.