bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan E-Government dalam Upaya Meningkatkan Kualitas Pelayanan Perizinan di Dinas Penanaman Modal dan Pelayanan Terpadu Satu Pintu (DPMPTSP) Kabupaten Karimun
Authors: Sulaiman Muhammad , Maysarah Maysarah , Wulandari Riski Peni
Published: July 2024

Electronic government (e-government) merupakan teknologi informasi dan komunikasi yang menjanjikan efisiensi, kecepatan penyampaian informasi, keterjangkauan, dan transparansi yang dapat digunakan untuk melakukan pendistribusian informasi dan layanan kepada masyarakat, perusahaan ataupun departemen pemerintahan lainnya oleh pemerintah nasional atau lokal melalui internet atau sarana digital lainnya. E-government bertujuan untuk mengatasi birokrasi pelayanan perizinan yang lamban, prosedur yang berbelit-belit, biaya tinggi, dan proses yang memakan waktu yang lama. Penerapan e-government untuk mengatasi permasalahan tersebut dan untuk meningkatkan kualitas pelayanan perizinan turut dilakukan oleh Dinas Penanaman Modal dan Pelayanan Terpadu Satu Pintu (DPMPTSP) Kabupaten Karimun. Penelitian ini bertujuan untuk mengkaji kualitas pelayanan perizinan di DPMPTSP Kabupaten Karimun. Metode penelitian yang dipilih adalah metode kualitatif deskriptif melalui pendekatan studi literatur dengan menelaah buku, jurnal dan artikel terkait e-government dalam pelayanan perizinan. Hasil dari penelitian ini menunjukkan bahwa pemanfaatan e-government di DPMPTSP Kabupaten Karimun menekankan integrasi antara government to government, government to bussines, dan government to citizens dalam satu sistem pelayanan satu pintu. Adapun cakupan e-government di DPMPTSP Kabupaten Karimun yaitu e-administration, e-citizen dan e-service. Penerapan e-government di DPMPTSP Kabupaten Karimun berhasil meningkatkan kualitas pelayanan perizinan menjadi lebih efektif dan efesien yang dibuktikan dengan semakin meningkatnya data statistik pengunjung dan pengguna layanan di website DPMPTSP Kabupaten Karimun setiap harinya.
Efforts to Increase Knowledge of Clean and Healthy Living
Authors: Rafika Aini  , Dewi Fitriani  , Makmur Hidayat  , Rahmadhani  , Maisharoh
Published: July 2024

Clean and Healthy Living Behavior (PHBS) is an action taken to improve individual and community health through daily habits that support cleanliness and health. This empowerment aims to understanding of PHBS through educational programs involving lectures, discussions and questions and answers. This program was held on 23-24 September 2023 and was attended by 57 grade 7 students. The pre-test and post-test results showed an increase in students' knowledge from an average score of 6.49 to 8.91, indicating the effectiveness of the educational program in increasing awareness and knowledge about PHBS.
The main emphasis in this program is the importance of washing hands properly to prevent disease, as well as the role of a clean school environment in supporting student health and learning achievement. The research conclusion shows that education about PHBS is effective in increasing students' knowledge, which in turn can contribute to creating a healthier school environment and supporting learning achievement. Support from families and schools, as well as the active role of the School Health Unit (UKS), is very important in maintaining and improving clean and healthy living behavior among students.
Features of the implementation of control (supervisory) activities in the field of the technical condition of self-propelled machines and other types of equipment by the State Technical Supervision bodies of the Russian Federation
Authors: Lomov Ivan Sergeevich
Published: July 2024

The year 2024 is a natural continuation of the reform of control (supervisory) activities, which began in 2020 in the Russian Federation. The concept of improving control (supervision) activities adopted by the Ministry of Economic Development until 2026 defined the final "philosophy" of control (supervision). It immediately became clear that the implementation of certain control (supervisory) functions is not possible without the reform of the activities of individual executive authorities performing these functions. The subject of our research is the specifics of the control (supervisory) activities of the State Technical Supervision bodies of the Russian Federation in 2024 under the conditions of the moratorium and the development of the concept of improving control (supervisory) activities until 2026. Our article aims to propose approaches that allow for the integration of supervision of self-propelled vehicles into a new reality aimed at reducing the pressure of the state, as a power machine, on economic entities. In addition, the subject of the study also includes the definition of generally applicable signs, tasks and principles of control (supervision). The definition of similarities and differences between the concepts of "control" and "supervision" does not remain without attention of the author. The scientific novelty of the study lies in the proposed ways to change the current activities of Gostekhnadzor bodies related to the implementation of control (supervision) in the field of the technical condition of self-propelled machines and other types of equipment, namely, amendments to the current legislation of the Russian Federation regulating this area. An important conclusion of the study should be the identification of the inapplicability of a risk-based approach to all types of control (supervision). In addition, we propose a structure of general requirements for the organization and supervision of self-propelled vehicles, as well as a change in the current version of Article 9.3 of the Administrative Code of the Russian Federation, corresponding to new realities and allowing, within the framework of a "permanent raid", to bring to administrative responsibility drivers of self-propelled vehicles that violate mandatory requirements in the field of their technical condition.
Performance of UV photodetector of mechanical exfoliation prepared PEDOT:PSS/β-Ga2O3 microsheet heterojunction
Authors: Yi Zi-Qi , Wang Yan-Ming , Wang Shuo , Sui Xue , Shi Jia-Hui , Yang Yi-Han , Wang De-Yu , Feng Qiu-Ju , Sun Jing-Chang , Liang Hong-Wei ,   School of Physics and Electronic Technology, Liaoning Normal University, Dalian 116029, China,   School of Integrated Circuits, Dalian University of Technology, Dalian 116024, China
Published: July 2024

Ultrawide-bandgap (4.9 eV) β-Ga2O3 material possesses exceptional properties such as a high critical-breakdown field (~8 MV/cm) and robust chemical and thermal stability. However, due to the challenges in the growth of p-type β-Ga2O3, the preparation of homojunction devices is difficult. Therefore, the utilization of heterojunctions based on β-Ga2O3 provides a viable approach for fabricating ultraviolet photodetectors. Poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS), a p-type organic polymer material, exhibits high transparency in a 250–700 nm wavelength range. Additionally, its remarkable conductivity (>1000 S/cm), high hole mobility (1.7 cm2·V–1·s–1), and excellent chemical stability make it an outstanding candidate for serving as a hole transport layer. Consequently, the combination of p-type PEDOT:PSS with n-type β-Ga2O3 in a heterojunction configuration provides a promising way for developing PN junction optoelectronic devices.

In this study, a β-Ga2O3 microsheet with dimensions: 4 mm in length, 500 μm in width, and 57 μm in thickness, is successfully exfoliated from a β-Ga2O3 single crystal substrate by using a mechanical exfoliation technique. Subsequently, a PEDOT:PSS/β-Ga2O3 organic/inorganic p-n heterojunction UV photodetector is fabricated by depositing the PEDOT:PSS organic material onto a side of the β-Ga2O3 microsheet. The obtained device exhibits typical rectification characteristics, sensitivity to 254 nm ultraviolet light, and impressive self-powering performance. Furthermore, the heterojunction photodetector demonstrates exceptional photosensitive properties, achieving a responsivity of 7.13 A/W and an external quantum efficiency of 3484% under 254 nm UV light illumination (16 μW/cm2) at 0 V. Additionally, the device exhibits a rapid photoresponse time of 0.25 s/0.20 s and maintains good stability and repeatability over time. Notably, after a three-month duration, the photodetection performance for 254 nm UV light detection remained unchanged, without any significant degradation. This in-depth research provides a novel perspective and theoretical foundation for developing innovative UV detectors and paving the way for future advancements in the field of optoelectronics.
Experimental study on boundary layer of internal flow visible supersonic nozzle
Authors: Zeng Rui-Tong , Yi Shi-He , Lu Xiao-Ge , Zhao Yu-Xin , Zhang Bo , Gang Dun-Dian ,   College of Aerospace Science and Engineering, National University of Defense Technology, Changsha 410073, China
Published: July 2024

The high-frequency pulsation noise generated by the turbulent boundary layer on the wall of a Laval nozzle can significantly affect the quality of the flow field at the nozzle outlet. In this study, a supersonic wind tunnel with visible internal flow is designed and fabricated to observe the development and evolution of the boundary layer on the contraction and expansion surfaces of a Laval nozzle, as well as to study the flow field inside the supersonic nozzle. The subsonic, transonic and supersonic profiles of the nozzle are designed by bicubic curve, Hall method and classical characteristic line method respectively. The results of numerical calculation and total pressure measurement show that the flow field at the nozzle outlet of the wind tunnel is uniform and stable, and the deviation of Mach-number-root mean square is better than the qualified level of China’s national military standard. Nanoparticle-tracer based planar laser scattering (NPLS) technology is used to carry out the flow display test of the internal flow visual supersonic nozzle, and the fine structure image of the whole flow field in the nozzle is obtained. The image clearly shows the development and evolution of the boundary layer in the nozzle. The interface between boundary layer and main stream and the wall curve of nozzle transition region are extracted by image processing technology. The fractal dimension of the extracted boundary layer contour is calculated, thereby establishing the corresponding relationship between the fractal dimension and the boundary layer state, and determining the transition position of the boundary layer. The results show that the transition position of the nozzle profile is closer to downstream than that of the nozzle straight wall. The fractal dimension can qualitatively judge the flow state of the boundary layer; however, it is necessary to distinguish between laminar boundary layers and hairpin vortices in the initial transition stage by considering the thickness of boundary layer.
Intuitionistic fuzzy sets in group decision making – A novel approach
Authors: Szmidt Eulalia ,   Systems Research Institute, Polish Academy of Sciences, Kacprzyk Janusz , Atanassova Vassia , Bujnowski Paweł ,   WIT Academy,   Systems Research Institute, Polish Academy of Sciences,   WIT Academy,   Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences,   Systems Research Institute, Polish Academy of Sciences
Published: July 2024

We use the natural properties of intuitionistic fuzzy sets (IFSs for short) to represent the pros, cons, and lack of knowledge concerning different options/alternatives, aiding in decision making, particularly in group decision making. We present a novel approach. We do not compare options/alternatives in pairs, we do not use distances. The approach is transparent and easily understandable for decision makers. The novel method points out the best option by ranking them.
On OWA, Machine Learning and Big Data: The case for IFS over universes
Authors: Chountas Panagiotis ,   School of Computer Science & Engineering, University of Westminster, Hajmohammed Mustafa , Rhemat Ismael ,   School of Computer Science & Engineering, University of Westminster,   School of Computer Science & Engineering, University of Westminster
Published: July 2024

This paper provides a holistic view of open-world machine learning by investigating class discovery, and class incremental learning under OWA. The challenges, principles, and limitations of current methodologies are discussed in detail. Finally, we position IFS over multiple universes as a formalism to capture the evolution in Big Data as part of incremental learning.
Ranking of factors affecting pricing in construction projects by intuitionistic fuzzy analytic hierarchy process with ordered pairs
Authors: Çebi Selçuk ,   Department of Industrial Engineering, Yildiz Technical University, Kahraman Cengiz , Onar Sezi Çevik , Öztayşi Başar ,   Industrial Data Analytics and Decision Support Systems Center, Azerbaijan State University of Economics,   Istanbul Technical University,   Istanbul Technical University,   Istanbul Technical University
Published: July 2024

The construction industry faces various challenges related to uncertainties in project bids and unstable pricing. These challenges include situations where uncertainties in bid documents can affect project pricing and performance. In order to resolve these uncertainties and clarify project requirements, risks and uncertainties within the document need to be identified early in the project life cycle. This study aims to rank the factors affecting project pricing. For this purpose, firstly, the parameters that have an impact on pricing are defined and then these factors are weighted by using Intuitionistic Fuzzy Analytic Hierarchy Process with Ordered Pairs. The analysis of factors affecting construction pricing reveals some important insights. Environmental uncertainties and uncertainties in prices and labor costs are the most important factors influencing pricing, while past project experience is the least important factor. Overall, these findings shed light on the multifaceted nature of construction pricing, which is shaped by the complex interaction of environmental, economic and market factors.
Software for Intercriteria Analysis results visualization in the intuitionistic fuzzy triangle: Opportunities for data interpretation
Authors: Genov Miroslav ,   “Prof. Dr. Assen Zlatarov” University, Bureva Veselina ,   Laboratory of Intelligent Systems, “Prof. Dr. Assen Zlatarov” University
Published: July 2024

In the current investigation a program implementation for data visualization into the intuitionistic fuzzy triangle according to the InterCriteria analysis notation is discussed. The presented software provides an opportunity for in-depth analysis of the results and flexible visualization of data obtained from the InterCriteria Analysis. An example of the software workflow is described
Mechanotransductive feedback control of endothelial cell motility and vascular morphogenesis
Authors: Mason Devon E. , Camacho Paula , Goeckel Megan E. , Tobin Brendan R. , Vega Sebastián L. , Wu Pei-Hsun , Johnson Dymonn , Heo Su-Jin , Wirtz Denis , Burdick Jason A. , Wood Levi , Chow Brian Y. , Stratman Amber N. , Boerckel Joel D.
Published: July 2024

Vascular morphogenesis requires persistent endothelial cell motility that is responsive to diverse and dynamic mechanical stimuli. Here, we interrogated the mechanotransductive feedback dynamics that govern endothelial cell motility and vascular morphogenesis. We show that the transcriptional regulators, YAP and TAZ, are activated by mechanical cues to transcriptionally limit cytoskeletal and focal adhesion maturation, forming a conserved mechanotransductive feedback loop that mediates human endothelial cell motility
in vitro
and zebrafish intersegmental vessel (ISV) morphogenesis
in vivo
. This feedback loop closes in 4 hours, achieving cytoskeletal equilibrium in 8 hours. Feedback loop inhibition arrested endothelial cell migration
in vitro
and ISV morphogenesis
in vivo
. Inhibitor washout at 3 hrs, prior to feedback loop closure, restored vessel growth, but washout at 8 hours, longer than the feedback timescale, did not, establishing lower and upper bounds for feedback kinetics
in vivo
. Mechanistically, YAP and TAZ induced transcriptional suppression of RhoA signaling to maintain dynamic cytoskeletal equilibria. Together, these data establish the mechanoresponsive dynamics of a transcriptional feedback loop necessary for persistent endothelial cell migration and vascular morphogenesis.
MDverse: Shedding Light on the Dark Matter of Molecular Dynamics Simulations
Authors: Tiemann Johanna K. S. , Szczuka Magdalena , Bouarroudj Lisa , Oussaren Mohamed , Garcia Steven , Howard Rebecca J. , Delemotte Lucie , Lindahl Erik , Baaden Marc , Lindorff-Larsen Kresten , Chavent Matthieu , Poulain Pierre
Published: July 2024

The rise of open science and the absence of a global dedicated data repository for molecular dynamics (MD) simulations has led to the accumulation of MD ﬁles in generalist data repositories, constituting the
dark matter of MD
— data that is technically accessible, but neither indexed, curated, or easily searchable. Leveraging an original search strategy, we found and indexed about 250,000 ﬁles and 2,000 datasets from Zenodo, Figshare and Open Science Framework. With a focus on ﬁles produced by the Gromacs MD software, we illustrate the potential offered by the mining of publicly available MD data. We identiﬁed systems with speciﬁc molecular composition and were able to characterize essential parameters of MD simulation such as temperature and simulation length, and could identify model resolution, such as all-atom and coarse-grain. Based on this analysis, we inferred metadata to propose a search engine prototype to explore the MD data. To continue in this direction, we call on the community to pursue the effort of sharing MD data, and to report and standardize metadata to reuse this valuable matter.
Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regeneration
Authors: Lu Yu-Ming , Yan Shan , Ti Shih-Chieh , Zheng Chaogu
Published: July 2024

Tubulin posttranslational modifications (PTMs) modulate the dynamic properties of microtubules and their interactions with other proteins. However, the effects of tubulin PTMs were often revealed indirectly through the deletion of modifying enzymes or the overexpression of tubulin mutants. In this study, we directly edited the endogenous tubulin loci to install PTM-mimicking or -disabling mutations and studied their effects on microtubule stability, neurite outgrowth, axonal regeneration, cargo transport, and sensory functions in the touch receptor neurons of Caenorhabditis elegans. We found that the status of β-tubulin S172 phosphorylation and K252 acetylation strongly affected microtubule dynamics, neurite growth, and regeneration, whereas α-tubulin K40 acetylation had little influence. Polyglutamylation and detyrosination in the tubulin C-terminal tail had more subtle effects on microtubule stability likely by modulating the interaction with kinesin-13. Overall, our study systematically assessed and compared several tubulin PTMs for their impacts on neuronal differentiation and regeneration and established an in vivo platform to test the function of tubulin PTMs in neurons.
Neurons in the ventral striatopallidal complex modulate lateral hypothalamic orexin/hypocretin neuron activity: Implications for reward-seeking
Authors: Mitchell Caitlin S. , Mohammadkhani Aida , Manning Elizabeth E. , Campbell Erin J. , Fisher Simon D , Yeoh Jiann W. , Pearl Amy J. , Burton Nicholas J. , Qiao Min , Iredale Jacqueline A. , Bains Jaideep S. , McNally Gavan P. , Andrews Zane A. , Graham Brett A. , Scammell Thomas E. , Lowell Bradford B. , Kong Dong , Borgland Stephanie L. , Dayas Christopher V.
Published: July 2024

Reward-seeking involves the engagement and computation of multiple physiological and motivational parameters. The lateral hypothalamus (LH) is a necessary node in the circuits that control food-seeking and motivation. One group of cells that plays an important yet incompletely understood role in these processes are the orexin/hypocretin (OX/HT) neurons. OX/HT cells are located exclusively within the LH and are implicated in feeding, arousal, and reward-seeking behavior. Importantly, the role of OX/HT neurons in consummatory versus reward-seeking actions is not fully defined, nor are the circuits that control the activity of these neurons under different behavioral states. Here we show that OX/HT neurons respond in real time to food presentation and that this response is modulated by differences in metabolic state. We observed increased OX/HT neuron activity on approach to food, but this activity trended towards pre-approach levels by the start of the consummatory phase. Next, we studied ventrostriatopallidal (VSP) inputs to the OX/HT neurons. Using optogenetics and cell type-specific electrophysiology, we found that ventral pallidum inputs onto OX/HT neurons exert strong inhibitory (and weak excitatory) effects whereas the lateral nucleus accumbens shell provides weaker direct inhibitory connections with OX/HT neurons. These findings reveal that the activity of OX/HT neurons is strongly modulated by metabolic and hedonic state. Further, OX/HT neurons is primarily associated with food approach and that the effect of VSP-terminal output is to suppress OX/HT activity.
Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development
Authors: Hussain Saman , Pinter Katherine , Uhl Mara , Wong Hiu-Tung , Kindt Katie
Published: July 2024

Sensory cells in the retina and inner ear rely on specialized ribbon synapses for neurotransmission. Disruption of these synapses is linked to visual and auditory dysfunction, but it is unclear how these unique synapses are formed. Ribbon synapses are defined by a presynaptic density called a ribbon. Using live-imaging approaches in zebrafish, we find that early in hair-cell development, many small ribbon precursors are present throughout the cell. Later in development, fewer and larger ribbons remain, and localize at the presynaptic active zone (AZ). Using tracking analyses, we show that ribbon precursors exhibit directed motion along an organized microtubule network towards the presynaptic AZ. In addition, we show that ribbon precursors can fuse together on microtubules to form larger ribbons. Using pharmacology, we find that microtubule disruption interferes with ribbon motion, fusion, and normal synapse formation. Overall, this work demonstrates a dynamic series of events that underlies formation of a critical synapse required for sensory function.
Rudhira-mediated microtubule stability controls TGFβ signaling during mouse vascular development
Authors: Joshi Divyesh , Jindal Preeti , Shetty Ronak , Inamdar Maneesha S.
Published: July 2024

The Transforming Growth Factor β (TGFβ) signaling pathway is critical for survival, proliferation, and cell migration, and is tightly regulated during cardiovascular development. Smads, key effectors of TGFβ signaling, are sequestered by microtubules (MTs) and need to be released for pathway function. Independently, TGFβ signaling also stabilizes MTs. Molecular details and the
in vivo
relevance of this cross-regulation remain unclear, understanding which is important in complex biological processes such as cardiovascular development. Here, we use
rudhira/Breast Carcinoma Amplified Sequence 3 (BCAS3)
, a MT-associated, endothelium-restricted and developmentally essential proto-oncogene, as a pivot to decipher cellular mechanisms in bridging TGFβ signaling and MT stability. We show that Rudhira regulates TGFβ signaling
in vivo,
during mouse cardiovascular development, and in endothelial cells in culture. Rudhira-MT association is essential for the activation and release of Smad2/3 from MTs. Consequently, Rudhira depletion attenuates Smad2/3-dependent TGFβ signaling thereby impairing cell migration. Interestingly, Rudhira is also a transcriptional target of Smad2/3-dependent TGFβ signaling essential for TGFβ-induced MT stability. Our study identifies an immediate early physical role and a slower, transcription-dependent role for Rudhira in cytoskeleton-TGFβ signaling crosstalk. These two phases of control could facilitate temporally– and spatially restricted targeting of the cytoskeleton and/or TGFβ signaling in vascular development and disease.
VGLL2 and TEAD1 fusion proteins drive YAP/TAZ-independent transcription and tumorigenesis by engaging p300
Authors: Guo Susu , Hu Xiaodi , Cotton Jennifer L. , Ma Lifang , Li Qi , Cui Jiangtao , Wang Yongjie , Thakare Ritesh P. , Tao Zhipeng , Ip Y. Tony , Wu Xu , Wang Jiayi , Mao Junhao
Published: July 2024

Studies on Hippo pathway regulation of tumorigenesis largely center on YAP and TAZ, the transcriptional co-regulators of TEAD. Here, we present an oncogenic mechanism involving VGLL and TEAD fusions that is Hippo pathway-related but YAP/TAZ-independent. We characterize two recurrent fusions, VGLL2-NCOA2 and TEAD1-NCOA2, recently identified in spindle cell rhabdomyosarcoma. We demonstrate that, in contrast to VGLL2 and TEAD1, the fusion proteins are strong activators of TEAD-dependent transcription, and their function does not require YAP/TAZ. Furthermore, we identify that VGLL2 and TEAD1 fusions engage specific epigenetic regulation by recruiting histone acetyltransferase p300 to control TEAD-mediated transcriptional and epigenetic landscapes. We showed that small molecule p300 inhibition can suppress fusion proteins-induced oncogenic transformation both
in vitro
and
in vivo
. Overall, our study reveals a molecular basis for VGLL involvement in cancer and provides a framework for targeting tumors carrying VGLL, TEAD, or NCOA translocations.
Dynamic basis of lipopolysaccharide export by LptB2FGC
Authors: Dajka Marina , Rath Tobias , Morgner Nina , Joseph Benesh
Published: July 2024

Lipopolysaccharides (LPS) confer resistance against harsh conditions, including antibiotics, in Gram-negative bacteria. The lipopolysaccharide transport (Lpt) complex, consisting of seven proteins (A-G), exports LPS across the cellular envelope. LptB2FG forms an ATP-binding cassette transporter that transfers LPS to LptC. How LptB2FG couples ATP binding and hydrolysis with LPS transport to LptC remains unclear. We observed the conformational heterogeneity of LptB2FG and LptB2FGC in micelles and/or proteoliposomes using pulsed dipolar electron spin resonance spectroscopy. Additionally, we monitored LPS binding and release using laser-induced liquid bead ion desorption mass spectrometry. The β-jellyroll domain of LptF stably interacts with the LptG and LptC β-jellyrolls in both the apo and vanadate-trapped states. ATP binding at the cytoplasmic side is allosterically coupled to the selective opening of the periplasmic LptF β-jellyroll domain. In LptB2FG, ATP binding closes the nucleotide binding domains, causing a collapse of the first lateral gate as observed in structures. However, the second lateral gate, which forms the putative entry site for LPS, exhibits a heterogeneous conformation. LptC binding limits the flexibility of this gate to two conformations, likely representing the helix of LptC as either released from or inserted into the transmembrane domains. Our results reveal the regulation of the LPS entry gate through the dynamic behavior of the LptC transmembrane helix, while its β-jellyroll domain is anchored in the periplasm. This, combined with long-range ATP-dependent allosteric gating of the LptF β-jellyroll domain, may ensure efficient and unidirectional transport of LPS across the periplasm.
Empowering AlphaFold2 for protein conformation selective drug discovery with AlphaFold2-RAVE
Authors: Gu Xinyu , Aranganathan Akashnathan , Tiwary Pratyush
Published: July 2024

Small-molecule drug design hinges on obtaining co-crystallized ligand-protein structures. Despite AlphaFold2’s strides in protein native structure prediction, its focus on apo structures overlooks ligands and associated holo structures. Moreover, designing selective drugs often benefits from the targeting of diverse metastable conformations. Therefore, direct application of AlphaFold2 models in virtual screening and drug discovery remains tentative. Here, we demonstrate an AlphaFold2-based framework combined with all-atom enhanced sampling molecular dynamics and Induced Fit docking, named AF2RAVE-Glide, to conduct computational model-based small-molecule binding of metastable protein kinase conformations, initiated from protein sequences. We demonstrate the AF2RAVE-Glide workflow on three different mammalian protein kinases and their type I and II inhibitors, with special emphasis on binding of known type II kinase inhibitors which target the metastable classical DFG-out state. These states are not easy to sample from AlphaFold2. Here, we demonstrate how with AF2RAVE these metastable conformations can be sampled for different kinases with high enough accuracy to enable subsequent docking of known type II kinase inhibitors with more than 50% success rates across docking calculations. We believe the protocol should be deployable for other kinases and more proteins generally.
Escritura figural. Una erótica de la imagen y la palabra en Mudanza, de Verónica Gerber
Authors: López Paz
Published: July 2024

Mudanza (2010) es el primer libro de Verónica Gerber (1981), un libro donde la autora narra la migración que cinco artistas realizaron desde la literatura al arte. En este libro podemos encontrar una suerte de programa de los modos en que la autora ha pensado los traslados entre escritura y visualidad a lo largo de todo su trabajo. En Mudanza, los textos abren una zona de intervalo, de indeterminación, una suerte de tierra de nadie en el corazón de la palabra y de la imagen, como si los escritores devenidos artistas sacrificaran los resultados de esa mudanza para poder explorar todo lo que acontece en ese traslado. Con esa misma ambigüedad escribe Gerber, componiendo textos a medio camino entre la literatura, el ensayo, la autobiografía, la escritura visual. Este artículo indaga en la pregunta por aquello que acontece cuando las imágenes y las palabras salen de sí mismas, perfilando lo que podríamos llamar una escritura figural que hace del intervalo el lugar de una potencia que trabaja en contra del estereotipo y el sentido unívoco.
Literatura y práctica de la pintura: análisis retórico-poético de un pasaje de L. B. Alberti
Authors: García Moggia Macarena
Published: July 2024

Este artículo propone un análisis filológico de un pasaje del tratado de pintura de León Battista Alberti, particularmente aquel donde el autor compara el cuadro con una «ventana abierta». De amplia supervivencia en las teorías modernas de la representación pictórica, tal expresión metafórica encontraría su origen en ciertos hábitos retóricos y poéticos tan propios del humanismo renacentista como singulares y originales en su aplicación teórico-práctica, mostrando la existencia de una alianza profunda entre las figuraciones literarias y los imaginarios visuales que se han asentado en nuestra tradición.
La influencia rossiniana más allá de Lima: Lorenzo Rojas, su música sacra y sinfónica (Arequipa, 1840-1865)
Authors: Vega Salvatierra Zoila
Published: July 2024

Inmediatamente después de producida la independencia política del Perú en 1824, el continente sudamericano abrió las puertas a la música de los operistas italianos de la época, con especial preferencia por Gioacchino Rossini. Su música no llegó necesariamente de la mano de compañías operísticas sino a través de oberturas transcritas para piano o ensambles de cámara y arias sueltas entonadas en salones y tertulias domésticas, arreglos para bandas de guerra interpretados en retretas en espacios públicos y bailes sociales que finalmente crearon un mercado adecuado para la construcción de nuevos teatros ávidos de recibir compañías líricas profesionales. Pero el fenómeno rossiniano no solo transformó la audición en los diferentes espacios sonoros de las ciudades sino que caló hondo en los compositores que adaptaron varios rasgos de este y lo incorporaron a su propia tradición y escuela. En algunas ciudades andinas de provincia como Arequipa, esta influencia se tradujo en la producción de obras destinadas a usos específicos distantes del espacio escénico, pero en cuya construcción se advierte la asimilación de este estilo a través de la adaptación de sus modelos melódicos y formales en géneros que el maestro de Pésaro cultivó en menor medida que la ópera, como el sinfonismo y la música litúrgica. A partir del estudio de música sacra y sinfónica de Lorenzo Rojas (1806-1865), violinista y maestro de capilla de la catedral de Arequipa, se aborda este fenómeno de asimilación y reutilización del lenguaje rossiniano en territorios americanos donde la funcionalidad y significado se transforman al perseguir ideales de modernidad sin renunciar a tradiciones y usos locales que habían ganado prestigio y aceptación públicos.
Articulación en la flauta traversa: una exploración desde la práctica artística utilizando herramientas del Alfabeto Fonético Internacional
Authors: Yáñez Mendoza Eduardo
Published: July 2024

La articulación en la flauta traversa se suele enseñar a través de distintas sílabas, tales como tu y du. Desde el siglo XVIII ya se encuentra evidencia de este recurso pedagógico. Esta forma de enseñanza ha implicado que la articulación varíe notablemente dependiendo del idioma y forma de pronunciación de cada flautista. Lamkin (2005), Helgeson (2012), Hartman (2019) y Tuley (2021), a través de distintas investigaciones, han explorado esta relación entre fonética y articulación en la flauta, concluyendo, entre otras cosas, que el aprendizaje de distintos idiomas puede ser beneficioso en la interpretación de este instrumento. Con base en estas investigaciones, el presente artículo se propone indagar en la influencia a nivel estético de la fonética aplicada a la articulación en la flauta traversa, utilizando el Alfabeto Fonético Internacional. Este sistema permite trabajar con fonemas antes que con idiomas particulares y explicar de manera más precisa el funcionamiento del tracto vocal al momento de tocar la flauta. Se concluye que a través de la investigación basada en la práctica artística es posible ofrecer una explicación más precisa de ciertas ideas tradicionalmente aceptadas acerca de la técnica e interpretación musical, como por ejemplo, el efecto de ciertos idiomas en la articulación de la flauta.
Cidades Inteligentes: as opiniões dos usuários do SUS sobre os serviços e as ferramentas online em duas Unidades Básicas de Saúde de Vitória (ES)
Authors: Borel Flávia Miranda Sarmento , Santos Glicia Vieira dos
Published: July 2024

[Introdução] A cidade de Vitória, capital do Espírito Santo, implementou na área da saúde, ferramentas e serviços considerados inteligentes. [Objetivo] Verificar quais são as opiniões dos usuários que utilizam o Sistema Único de Saúde (SUS) desse município, em duas Unidades Básicas de Saúde (UBS), sobre os serviços e as ferramentas inteligentes implementados. [Metodologia] O trabalho utiliza abordagem quantitativa e qualitativa. Foram realizadas aplicações de questionário com os usuários que utilizam o SUS na UBS localizada no bairro de Ilha de Santa Maria e na UBS localizada no bairro de Santo André, e entrevistas semiestruturadas com servidores e conselheiros de saúde vinculados a essas UBS’s. Os dados foram analisados por meio de estatística descritiva. [Resultados] O novo formato de oferta de serviços e ferramentas tecnológicos proporcionou muitas opiniões positivas, comuns aos usuários das duas UBS’s, tais como a melhoria nos serviços ofertados e na qualidade de vida. Todavia, a tecnologia não resolveu problemas básicos, como a má qualidade da internet, a dificuldade para agendamento de consultas por meio do aplicativo e a falta de preparo – de usuários e de servidores – para utilizar os novos serviços e tecnologias. Além disso, são UBS’s que atendem usuários com perfis e realidades sociais distintas.
Safety and Effectiveness of Bone Allografts for Mastoid Obliteration After Mastoidectomy: A Pilot Study
Authors: Kim Woo Jin , Park Chanhyeon , Sim Soohyun , Hong Tae Ui , Park Sung Yool , Heo Kyung Wook
Published: July 2024

Background and Objectives: Various materials are used to perform post-mastoidectomy mastoid obliteration (MO) to reduce the risk of recurrent infections, stasis of secretions, or caloric dizziness. Autologous materials used as fillers for MO tend to be insufficient owing to shrinkage over time or inadequate volume of these substances. Synthetic materials are unsatisfactory for MO because of the risk of rejection and extrusion. We investigated the safety and effectiveness of bone allografts for post-mastoidectomy MO.Subjects and Methods: We reviewed the medical records of patients who underwent mastoidectomy with MO between January 2013 and January 2021. In the MO group, bone allografts were additionally used to fill the residual mastoid cavity. In the canal wall down (CWD) group, all patients underwent CWD mastoidectomy with use of additional inferiorly based mucoperiosteal flaps.Results: The study included the MO group (23 ears) and the CWD group (53 ears). In the MO group, compared with the preoperative status, we observed a decrease in the tendency of the air-bone gap postoperatively. Compared with the CWD group, the total complication rate showed a decreasing tendency in the MO group.Conclusions: No patient showed post-MO shrinkage of the grafted bone allograft or otorrhea. Further large-scale studies are warranted to confirm the advantages of bone allografts for MO, including maintenance with time and sufficient amount.
A Brief Overview of Technologies in Automated Agriculture: Shaping the Farms of Tomorrow
Authors: Raj Ritik , Kumar Shailesh , Lal Sudhanand Prasad , Singh Hemlata , Pradhan Jyostnarani , Bhardwaj Yash
Published: July 2024

As global population continues to grow, there is an increasing need for innovative solutions to enhance agricultural productivity, efficiency, and sustainability. To meet increasing population demand, agricultural production must be doubled. The global population is projected to rise by almost two billion individuals within the next three decades. With global challenges such as population growth, climate change, and resource formation, automation in farming practices is one of the key driving forces behind this revolution. Robotics, coupled with artificial intelligence (AI) and advanced data analytics, are transformative solutions for precision farming and smart farming technologies. These technologies enable continuous and efficient farming operations and provide detailed monitoring at a plant-by-pant level, optimizing resource use and reducing the environmental footprint. Technological advancements have led to the development of various robotic systems, including agricultural grippers and autonomous machinery, which are integral to the automation of farming tasks, from sowing to harvesting. However, the adoption of such technologies is not without challenges. High initial investment costs, connectivity issues, and data security are some of the barriers that need to be addressed. The potential benefits of reduced operational costs, improved crop quality, and enhanced farm output make it a promising solution for the future of farming. In this article, we discuss the multifaceted role of robotics in modern agriculture by exploring both technological advancements and challenges to widespread adoption.
Correlation Studies on Floral Behavior of Chironji Populations (Buchanania lanzan Spreng) from Natural Forest Northern India
Authors: Kumar Siddharth , Srivastava A. K. , Rai Ashutosh , Singh Chandra Mohan , Tiwari Chandrakant , Singh Subhash Chandra , Prakash Om
Published: July 2024

The present investigation was conducted during 2022-23 and 2023-24. Survey was carried out across various districts in Uttar Pradesh, Madhya Pradesh, Bihar, and Gujarat, recording latitude and longitude for data collection and identification. Data were collected using minimal descriptors for characterization and evaluation of agri-horticultural crops, following PPV & FRA guidelines. Floral characteristics were recorded in 74 chironji accessions. The colour of fully bloom flower was recorded, 59 accessions noted creamy white with pink tinge, while 15 had creamy white flowers. The average number of flowers per inflorescence ranged from 1,954 to 3,650, with mean of 2,732. The average inflorescence length varied from 14.23 cm to 28.50 cm with mean of 20.23 cm and the average inflorescence width ranged from 15 cm to 34.55 cm with mean of inflorescence with 26.47 cm. The average number of peduncles per inflorescence ranged from 5.50 to 13.50, with mean of 10.00. Significant positive correlations were found among the floral characteristics. The average number of flowers per inflorescence strongly correlated with average inflorescence width (r = 0.809, p < 0.01) and moderately with the average number of peduncles per inflorescence (r = 0.543, p < 0.01) and average inflorescence length (r = 0.476, p < 0.01). The average inflorescence length showed a moderate correlation with average inflorescence width (r = 0.540, p < 0.01) and a weak correlation with the average number of peduncles (r = 0.233, p < 0.05). The average inflorescence width had a strong correlation with the average number of flowers per inflorescence and a moderate correlation with the average number of peduncles (r = 0.453, p < 0.01).
A Study on the Marketing Efficiency of Broiler in Bhagalpur District of Bihar, India
Authors: Patway Saurabh Kumar , Ramchandra
Published: July 2024

A study on broiler marketing was carried out in the Bhagalpur district of Bihar, and the results were presented using tabular analysis. A multi-stage stratified sampling method was utilized to select marketing intermediaries, encompassing those in both primary and secondary markets. The study focused on these intermediaries to collect data on total marketing costs, marketing margins, price spreads, marketing efficiency, and the producer's share of consumer rupees. Additionally, the research aimed to identify the various marketing channels used in the broiler marketing process. The study was undertaken through a bench mark survey method by contacting 70 broiler farms, representing small, medium and large farms. The average number of birds was 280 on small farms, 780 birds on medium farms. and 2970 on large farms. It was clear that the highest being on large farms indicating direct relationship with the farm size.
Variation in the Pullout Capacity according to the Tilt and Misorientation of a Floating Offshore Wind Turbine Suction Anchor Using Geometric Method
Authors: Kim Dae-Hwan , Jung Na-Young , Lee Won-Hyo , Kim Tae-Hyung
Published: July 2024

In this study, geometric calculation was performed to investigate the influence of the combined effect of tilt and misorientation on the pullout capacity of suction anchor used in floating offshore wind turbine. When considering the combined effect of tilt and misorientation, it was observed that they do not proportionally affect the pullout capacity but rather influence each other relatively. Criteria considering both tilt and misorientation are not provided in existing literature or DNV recommendation. Therefore, based on the comprehensive results of this study, a proposed decrease in pullout capacity within 3% is suggested.
AS DRAMÁTICAS DO USO DO CORPO-SI DOCENTE NA PANDEMIA:
Authors: Souza de Vargas Rosana , Freitas Ernani Cesar de , Amarante Priscila de Queiroz
Published: July 2024

Este artigo tem como temática a interface Linguagem e Trabalho com base nas conjecturas teóricas da Ergologia. O objetivo é identificar e analisar as dramáticas do uso do corpo-si, os saberes constituídos e investidos, as renormalizações e renormatizações enfrentadas por professores de Letras da educação básica durante o ensino remoto e no retorno às aulas presenciais em 2021. O estudo pauta-se em conceitos advindos da temática mencionada, como: noção de atividade, tarefa e trabalho, dramáticas do uso de corpo-si no trabalho, saber constituído e saber investido, renormalizações e renormatizações. Contempla uma abordagem qualitativa por meio de uma pesquisa aplicada exploratória e de campo. Foi realizado um levantamento bibliográfico e procedeu-se a uma Análise Textual Discursiva (ATD) de entrevistas semiestruturadas com três docentes de Letras. Como resultados, evidencia-se que há uma intensificação das práticas trabalhistas docentes identificadas nas dramáticas do uso de si por si e pelos outros, que pressupõe sobrecarga laboral e esgotamento mental e psicológico.
WHEN CLITIC SYSTEMS COLLIDE, ANIMACY, FOR THE MOST PART, BEATS GENDER
Authors: Schmitt Cristina , Primucci Ana Eleonora , Munn Alan , Brandani Lucia , Avellana Alicia
Published: July 2024

In this paper we present elicitation results from an experiment on the acquisition of 3rd person object clitics by children of Paraguayan parents living in Buenos Aires and their mothers. These children are immersed in a situation of contact between two varieties of Spanish: the Paraguayan system (∅ for inanimate objects and LE for animate objects) and the local Argentinian system which uses LO for Masculine and LA for Feminine clitics. Results show that the Paraguayan mothers use substantially more LO/LA when compared with adults in Paraguay, as described by Choi (2000), suggesting a great deal of attrition. Their children on the other hand use far less LO/LA, but at the same time are not adopting as a group a LE/∅ system. Examining 19 Mother/Child dyads, we find that no children are exclusively using LO/LA users but some children are exclusively using LE/∅. As for mother we find some LO/LA users and the rest uses a mixed system. Interestingly, overall children use fewer forms than mothers.
San Diego's Area Coordinator System: A Disaster Preparedness Model for US Nursing Homes
Authors: Blake Sarah C. , Howard David H. , Eiring Hilary , Tarde Scott
Published: December 2012

Almost 2 million Americans rely on nursing homes for care, and many require daily or near daily contact with the health care system to remain alive and functional. In October 2007, Southern California experienced a series of wildfires that burned over 500 000 acres and caused 14 nursing homes to evacuate more than 1200 residents. In response to this event, nursing home administrators and officials from various health care and emergency management agencies in San Diego County collaborated to form a model for nursing home emergency preparedness. This report describes the model, known as the area coordinator system, and discusses its strengths and limitations, and whether it ought to be replicated in other areas of the country.

(Disaster Med Public Health Preparedness. 2012;6:424-427)
An Ullmann CO Coupling Reaction Catalyzed by Magnetic Copper Ferrite Nanoparticles
Authors: Yang Shuliang , Wu Cunqi , Zhou Hua , Yang Yanqin , Zhao Yongxia , Wang Chenxu , Yang Wei , Xu Jingwei
Published: December 2012

Herein, an efficient method for the Ullmann CO coupling reaction between various kinds of phenols and aryl halides, including amino, ketone, cyano, methyl, methoxy, fluoro, chloro and bromo derivatives, is described. The catalyst used, copper ferrite (CuFe2O4) nanoparticles, are easily made, air‐stable, and of low cost. The catalyst can be recycled easily just by using an external magnet. Even in the presence of sensitive substituents, the reaction proceeds successfully to provide the desired products in high yields without protection of other functional groups.
Ultrafast microfocus x‐ray source based on a femtosecond laser‐triggered tip
Authors: Foreman Seth M. , Kealhofer Catherine , Skulason Gunnar E. , Klopfer Brannon B. , Kasevich Mark A.
Published: December 2012

A compact microfocus x‐ray source based on a high repetition rate, ultrafast laser‐triggered field emission cathode is characterized. Bremsstrahlung and Cu Kα x‐ray pulses are generated in a transmission geometry from 20‐keV electrons incident on a thin Cu target. Timing correlations between optical and x‐ray pulses show that the precision timing properties of the optical pulse train are transferred to the x‐ray pulse train. Simple electron optics allow a demonstration of point‐projection x‐ray imaging. Modelling of the electron trajectories shows that sub‐µm spot sizes and sub‐100‐fs x‐ray pulse durations are feasible with improved electron optics.