bluuebunny/crossref_metadata_2025 huggingface dataset:
Análise de dados qualitativa: um olhar para o primeiro e segundo Ciclo de Saldaña
Authors: Anselmo Guilherme Alexandre , Cheron Cibele
Published: October 2023

Highlights * A escolha do método de codificação adequado é uma decisão a ser tomada com base no arcabouço e nos objetivos de cada estudo e sempre deve poder ser revista. * Na pesquisa qualitativa, as questões de validade, confiabilidade e replicabilidade ainda são vistas como desafios. * Incumbe ao pesquisador descrever com acuidade, de modo detalhado e reflexivo, a metodologia de abordagem qualitativa empregada, sempre em consonância com a moldura teórica conceitual e os objetivos da pesquisa. * O exame de um fenômeno é mais bem-sucedido se puder ser abordado por vários métodos diferentes, não hierarquizados.
Redação de artigos na área das Ciências da Vida
Authors: César Bicca-Marques Júlio
Published: October 2023

Highlights * O título deve ser informativo e atraente para o leitor ter interesse em ler o artigo. * O resumo é uma miniatura do artigo redigido na mesma sequência lógica do artigo. * A introdução descreve o que sabemos e o que queremos saber sobre o tema da pesquisa. * Os resultados descrevem os achados da pesquisa em forma de texto, tabelas e figuras. * A discussão apresenta a interpretação dos resultados e suas implicações teóricas.
“Cinema was becoming more real”
Authors: Miao Ruomu
Published: October 2023

This study examines the historical legitimacy, aesthetic potential and limitations of the vertical screen image through two dimensions: cross media comparison of the New Wave of French cinema and the creative practice of thevertical screen image in the complex new media environment of today. This studyfollows the principle of unity between history and logic to explore in detail theinfluence and inspiration of the narrative aesthetics of French New Wave films on the creation of short videos in the vertical screen era, starting from the four dimensions of “telling” and “showing”, as well as narrative time and narrative space. Through the media archaeology of film history, it is easy to find that many features of short video creation coincide with the creative concept advocated by the French New Wave.
Genotype distribution of hepatitis C virus in the province of Gaziantep, a 10-year evaluation
Authors: Cırıt Osman Sezer , Demir Yelda , Yıldırım Mehmet Saıt , Alpaslan Buket , Avcıoglu Fatma , Doğan Yusuf , Astam Pınar
Published: October 2023

Hepatitis C virus (HCV) can cause both acute and chronic hepatitis infections. Gaziantep is located southeast part of Turkey and has a border with Syria. More than 400,000 Syrian refugees live in Gaziantep. The aim of this study was to evaluate distribution of HCV genotypes among Syrian patients and in people who inject drugs.

Serum samples form 1,628 individuals (786 female, 842 male) which were sent to our laboratory for genotyping between January 2013 and December 2022, were analyzed retrospectively. Three different HCV genotyping assays (Qiagen, RTA and Abbott) were used during the 10-year study period.

Out of the 1,628 patients, genotype 1 was detected in 51.5%, genotype 3 in 21.4%, genotype 4 in 20%, genotype 5 in 4.6%, genotype 2 in 1.3%. Mixed genotype was found in 20 patients. Of the patients, 1,143 were Turkish patients and among those patients genotype 1 (66.8%) was the most common genotype followed by genotype 3 (29%). Among Syrian patients (n = 477), genotype 4 (64.2%) was predominant genotype followed by genotype 1 and genotype 5. Genotype 3 was detected in 277 (79.6%) prisoners. All of them were male and probably the main source of HCV infection was intravenous drug abuse. While genotypes 1 and 4 were common in females, genotypes 1 and 3 were common in males.

In the future genotype 3 may become an increasing problem due to the persons who inject drugs. Less frequent genotypes such as 4 and 5 may become more frequent due to Syrian patients.
Knowledge of recommended technologies by mandarin growers
Authors: Lohe R.G. , Mokhale S.U. , Chorey A.A. , Mouje D.D. , Dhengale A.S.
Published: October 2023

The present investigation was conducted in Narkhed Panchayat Samiti of Nagpur district (Vidarbha region) of Maharashtra state. The main objective of the study was to assess the relationship between selected characteristic of recommended technologies by mandarin growers. A pre – structured interview schedule was used to collect data from 120 mandarin growers from 10 villages which were selected by using exploratory design of the social research during 2021-2022. The statistical methods such as frequencies, percentage, mean, standard deviation and correlation were used. The data of research study show that age and irrigation source was negatively correlated and annual income, extension contact, innovativeness, risk preference were from found to be positively and significantly related with knowledge of recommended technologies by mandarin growers.
Development and quality evaluation of Shrikhand Gola (Gulabajamon from Chakka)
Authors: Kadu Kavita , Darade Rohini , Sarap Kalyani
Published: October 2023

The experiment was conducted to standardize the procedure for preparation of best quality gulabajamon using chakka without reducing its acidity and moisture by heat treatment. The different properties of maida in combination with chakka are 30%, 40% and 50% by following the Baking powder in the ration of 1.5% 2% and 2.5%.
A comprative study of chemical qualities of raw milk of goat and sheep
Authors: Shukla U.K. , Pandre Princy
Published: October 2023

A comprative study of chemical qualities of raw milk of goat and sheep was conducted at Livestock production and management (unit), Department of NRM, Faculty of agriculture, MGCGV Chitrakoot – Satna (M.P.) during January to April 2022. The objective was to find out the comparative chemical qualities of raw milk of goat and sheep for three animal each viz., goat and sheep for ten days as replication different parameter were subject to statistical analysis applying the technique of analysis of variance (f-test) the most widely used method for determining protein content by kjeldahi method for nitrogen determination since nitrogen is a characteristic can be finding. In view of the finding and results presented above, it may be concluded that the chemical quality of milk of sheep was superior than goat milk, due to higher protein, specific gravity, fat content, lactose, total solid and solid not fat, and lower ash and water content in sheep milk.
Problems as perceived by membes and non-members of dairy co-operative societies in Gadchiroli district of Vidarbha region
Authors: Kadu Kavita , Darade Rohini , Sarap Kalyani
Published: October 2023

Studies on problem / constraints faced by dairy farmers revealed that the major problems faced by majority of dairy farmers were related to health, followed by monitory and lastly related to facilities. Problem of high incidence of repeated breeding was mentioned by 76 % and 90.7% of beneficiary and non-beneficiary farmers followed by high incidence of prolapsed of uterus 70% and 84.7% of beneficiary and non-beneficiary farmers. High cost of concentrates and high cost of dairy animal was expressed by 69.3% and 53.3% of beneficiary and 69.13% and 78% of non-beneficiary farmers. Irregularity in payments was mentioned by maximum non-beneficiary farmer (86.7%), whereas only 7.3% of beneficiary farmer mentioned irregularity in payments as constraints for them. The problem related to health and money are major in both beneficiary and non-beneficiary farmers where as untimely payments of bills is major problems for non-beneficiary and it is negligible problem for beneficiary farmer as co-operatives pay them regularly.
Development and nutritional evaluation of product prepared from soy-malt flour
Authors: Rastogi Manvi , Chauhan Jyoti
Published: October 2023

This thesis is aimed at development nutritional value added food products with the addition of soy- malt flour. Intake of soy is very likely to help us to maintain our overall health in the long term. The whole grains are rich in protein, vitamin, minerals, iron, Calcium and amino acids essential for our health. More importantly soy or malt is one of the richest sources of both soluble and insoluble fibres. The work was designed to evaluate the scope of utilizing soy and malt flour which has been underutilizing food product. Soy and malt flour with 5 replication of each was done to assist the sensory acceptability of the product. Sensory score of various treatments were graded on 9 points hedonic scale scorecards. Sensory score of Cutlet were best and were most acceptable. Sensory scores of Sev were also acceptable. From our study, it was observed that small and malt flour product is compact source of nutrients. These products play quite significant role in now a days and it is beneficial to all age group.
Assessment of the effects of bio-organic nutrient management on the yield of Sharbati wheat varieties
Authors: Nandeha Nirjharnee , Kewat M. L.
Published: October 2023

Combined with vermicompost on the yield of Sharbati wheat cultivars, a field study was carried out in the Department of Agronomy, JNKVV, Jabalpur.In comparison to single application, the study discovered that adding vermicompost to bioorganic treatments like BD500, BD501, and panchgavya enhanced wheat yield characteristics and seed production. The Sharbati wheat variety JW3020 fared better than other kinds in an organic production system, having greater yield metrics (tillers 288.30 m2, grains/ear head 28.71, test weight 35.6 g, and ear length 7.40 cm). When all bio organic preparation (VC+BD500+BD501+Pancha gavya) were used in conjunction with the wheat variety JW3020, as opposed to other combinations of wheat types and the usage of other bio organic preparation, vermicompost produced the best results.
Controllability analysis of renewable embedded AC microgrid with multi-band stabilizers
Authors: Gaur Dhairya , Bhadu Mahendra , Bansal S.K. , Gaur J.K.
Published: October 2023

The depletion of conventional energy sources and the increasing demand for electricity due to industrialization and rising global living standards has led to the need for a transformation of the traditional power grid. The microgrid concept has emerged as a solution that integrates renewable energy sources into the main grid. This paper examines an AC microgrid consisting of solar, wind, hydro, and diesel power stations, and investigates the effect of various power system stabilisers (PSSs) on the silent-pole synchronous generator of the diesel and hydro power plant. The effectiveness of different PSSs, including - PSS, ΔPa-PSS, and Multi-band PSS, is compared using a grid-connected AC microgrid. The simulation results show that Multiband PSS performs better than conventional PSSs in eliminating electromechanical oscillation, even in the presence of Gaussian and colored noise. PSSs are useful in managing the power flow of various DERs and improving power system stability. The simulation is conducted using the MATLAB/SIMULINK software.
Morphometric analysis of watershed
Authors: Ramatal Megharaj , Kothari Mahesh , Singh P. K. , Singh Manjeet , Chhipa Brij Gopal
Published: October 2023

The analysis of drainage basins or watersheds based on morphometric parameters is highly significant for effective watershed planning. It provides valuable insights into the relationships between different characteristics within a given area. Although numerous technical papers have explored this field of study, there is currently no standardized classification system and clear interpretation for each parameter. Consequently, evaluating the significance of individual morphometric parameters can be confusing. This paper aims to address this issue by offering a comprehensive understanding of the meaning behind the values of various morphometric parameters, along with relevant contextual information. A critical review is provided for each classification, including the range of values and their implications. Emphasizing the importance of data quality, covering aspects such as data preparation, scale, and level of mapping detail is also done. This review paper is intended to provide a comprehensive explanation that will assist future researchers in their morphometric analysis studies.
Digital marketing of agriculture inputs- An institutional success story
Authors: Kumar P. B. Pradeep , Rao K. Tejeswara , Bharathalakshmi M. , Rambabu P.
Published: October 2023

This study highlights the institutional success achieved through the digital marketing of liquid biofertilizers, produced by RARS, Anakapalle, affiliated with Acharya N.G. Ranga Agricultural University, India, using the Amazon e-marketing platform. The strategic choice of Amazon was driven by its positive reputation, extensive customer reach, and efficient product delivery system. Five biofertilizer variants were made available through dedicated Amazon seller links, catering to farmers, NGOs, research stations, and extension centers. Customer feedback emphasized the time-saving aspect, year-round availability, convenience of purchase, prompt delivery, affordability, high-quality packaging, and inclusion of scientific information. These results underline the need for widespread promotion of online marketing channels, as they offer significant benefits such as time efficiency, 24/7 availability, home delivery, diverse product choices, and cost-effectiveness. Overall, digital marketing of agriculture inputs emerges as a highly valuable and useful approach for farmers, enhancing their access to essential resources.
Prevalence of Individuals with Traumatic Spinal Cord Injury in Nairobi, Kenya
Authors:   Head of Department Rehabilitation Medicine, Amref International University, Nairobi, Kenya., Muya Christine , Phillips Julie ,   Director of Research Development and Postgraduate Support, University of the Western Cape, Nairobi, Kenya., Matheri Joseph ,   Head of Department Physiotherapy, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya., Bilton Sore ,   Clinician at The Nairobi Hospital, Nairobi, Kenya.
Published: October 2023

Objectives: To determine the prevalence of individuals with a traumatic spinal cord injury in Nairobi, Kenya. Methods: Retrospective quantitative data was extracted from records of 1st January 2010 to December 2014, at the NSIH. (N=320) participants met the inclusion criteria of all TSCI patients injured archived medical files, out these (n=217) were men, and (n=103) women, collected data was analyzed by SPSS version 23.0. Pearson chi-square was applied to test for association between variables, Student t-test was used to compare mean difference between groups, study Significance level was at p-value <0.05. Results: Mean age of the participants was 37.59 (SD= 15.038), the highest percentage age was 30-39 years old at (27.8%) followed aged 18-29 years at (26.6%). Male to female ratio was 2.1:1, highest prevalence occurred in 2010 and 2014 (20.5%), main cause of a TSCI transportation (49.1%), fall at (33.4%), assault (17.5%) common injury location was at lumber (53.1%), followed thoracic at (27.5%) cervical and sacral at (19.1%) and (0.3%) respectively. There were significantly more persons with paraplegia (54.1%) who had complete injuries than those with tetraplegia (19.6%). Other (27.3%) accounted for the TSCI patients with incomplete paraplegia and tetraplegia and no neurological deficits Conclusions: TSCI is a devastating condition to individuals; it has a high impact on QOL and ADL in low-income countries where there is an increase in manual labor and poor infrastructure, which predispose individuals to TSCI. Further studies need to be done to understand and compare epidemiological results, to inform appropriate prevention strategies that will decrease the burden of TSCI globally. KEYWORDS: Traumatic Spinal Cord Injuries, Activities of Daily Living, Gun Short Wound, National Spinal Injury Hospital, American Spinal Injury Association impairment scale.
THE REJECTION OF ROE v WADE BY THE UNITED STATES SUPREME COURT AFTER FIVE DECADES – A SEISMIC DECISION
Authors: George Barrie
Published: October 2023

The recent dramatic about-turn of the United States Supreme Court (USSC) in Dobbs v Jackson Women’s Health Organisation (Dobbs) in May 2022 regarding the rights of women in the United States to an abortion has caused a major uproar. Overruling one of the most famous of all USSC cases, Roe v Wade (410 US 113 (1973) (Roe)), five decades after it was decided, has had major repercussions. To fully comprehend the impact that Roe had on the United States, it is opportune to give an overview of Roe, which was a dramatic judgment and became established law for 50 years. In revisiting Roe, it is difficult to understand why such a clear and well-reasoned majority judgment has been overruled.Roe raised serious legal, moral and religious issues. The central issue was whether a woman had a legal right to an abortion. The USSC by a majority of seven to two held that woman had a constitutionally protected right to an abortion. As will be seen, Roe based its decision on its interpretation specifically of the Fourteenth Amendment of the United States Constitution.All major cases before the USSC can be put into two categories – abortion cases and all others. Abortion remains the central legal issue before that court. It defines the judicial philosophies of the justices of the USSC. It dominates their nomination and confirmation processes. The abortion controversy is sensitive and emotional. It generates vigorous, opposing views. It inspires deep and absolute convictions. A person’s philosophy, experiences, exposure to the raw edges of human existence, religious beliefs, and attitudes towards family values, and the moral standards a person establishes and seeks to observe all influence their thinking about abortion.It arguably delineates the difference between the National Democratic and Republican parties. When, during the confirmation proceedings of the present Chief Justice Roberts, he was asked about his views on Roe, he was careful not to commit himself. His answer was that Roe was settled as a precedent of the court and entitled to be respected under the principles of stare decisis, but he added that the justices of the Supreme Court do sometimes reverse their own precedents.Abortion issues remain eternal. Does a woman have a legal right to an abortion? Is the termination of a pregnancy a decision to be made by a woman and her doctor, or is the protection of potential human life a legitimate interest of the State? At what point does an unborn person acquire legal rights that are protected under the law? The majority and dissenting judgments in Roe represent the full spectrum of legal approaches to these and related questions. However, no court decision exists in a vacuum and the controversial issues raised in Roe continue to be raised and debated in the legal and political life of the United States, as they do in other jurisdictions.
THE NATURE AND AMBIT OF THE INTIMIDATION OFFENCE S v White 2022 (2) SACR 511 (FB)
Authors: Shannon Hoctor
Published: October 2023

The offence of intimidation has been associated with controversy, particularly because of the historical link between the Intimidation Act (72 of 1982) and the legislative machinations of the apartheid regime. In the words of Gamble J, the Act may be regarded as “a piece of apartheid order legislation introduced at a time of increasingly repressive internal security legislation designed to criminalise conduct, largely in the field of resistance politics” (Sandlana v Minister of Police 2023 (2) SACR 84 (WCC) par 34). The nature and ambit of the intimidation offence has once again come under scrutiny in the recent case of S v White (2022 (2) SACR 511 (FB)). The decision in this case is examined here in the context of a general assessment of the offence. The offence can now only be committed by contravening section 1(1)(a) of the Act, as the Constitutional Court has struck down the section 1(1)(b) provision (as well as section 1(2)) as unconstitutional in Moyo v Minister of Police (2020 (1) SACR 373 (CC)), a development confirmed by the amendment of the Act by the Protection of Constitutional Democracy Against Terrorist and Related Activities Amendment Act (23 of 2022). (Some are of the view that the Constitutional Court could have gone further (Burchell Principles of Criminal Law 5ed (2016) 593–594).) However, for the purposes of the discussion that follows, it is useful to cite the full section 1(1) provision prior to amendment.
Wnt-3a and R-spo1 conditioned media reporter assay v2
Authors: Martagón Natalia , Kliem Gurdrun
Published: October 2023

Protocol designed to measure the activity of Wnt-3a or R-spondin-1 (Rspo1) conditioned media. A reporter HEK cell line expressing luciferase under Wnt-3a stimulation is cultured with conditioned media followed by cell lysis and a luciferase reporter assay. Activity is compared to previous media batches or references.
Evaluating the SWS bolted connection strength of Bitis wood
Authors:   Faculty of Engineering, Universiti Malaysia Sarawak, Sukarman Erwan , Karim Abdul Razak Abdul ,   Faculty of Engineering, Universiti Malaysia Sarawak, Sa’don Norazzlina M. ,   Faculty of Engineering, Universiti Malaysia Sarawak, Abdullah Abdul Azim ,   Faculty of Engineering, Universiti Malaysia Sarawak, Sahari Siti Hanim ,   Research, Development and Innovation Division, Forest Department Sarawak, Rahim Nur Liza ,   Faculty of Civil Engineering Technology, Universiti Malaysia Perlis, Quenneville Pierre ,   Faculty of Engineering, The University of Auckland
Published: October 2023

The current study was conducted to evaluate the strength of the steel-wood-steel (SWS) bolted connections of Bitis wood. The wood was chosen because it is commonly used as the structural elements of the as-built floor and roof diaphragms in Malaysian unreinforced masonry (URM) buildings. This present work was performed to continue the initiation of the wood database development for the purpose of retrofitting the wall-diaphragm connections of the URM building. The bolted connection testing was the main experimental investigation, where a total of eighteen groups of different connection configurations were tested, each consisting of ten specimens. The embedding strength tests, moisture content and density tests were also conducted to determine the wood's basic properties. The results obtained from this experimental study were utilized for verifying the prediction effectiveness of the existing design equations, namely the Malaysian Timber Standard (MS544-5), the European Yield Model (EYM), and the Row Shear Model (RSM). From the comparison made between the prediction values and the experimental results, the MS544-5 was found to be too conservative, whereas a combination of the EYM and RSM is recommended for predicting the bolted connection strength of the Bitis wood.
The efficiency of phytase and impact-disintegrator-activator processing of soybean for reducing phytic acid in soy protein hydrolysates
Authors:   ITMO University, Chelombitkin Mikhail A. , Barakova Nadezhda V. ,   ITMO University, Sharova Natalia Yu. ,   All-Russia Research Institute for Food Additives – Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, Baskovtceva Angelina S. ,   ITMO University, Samodelkin Evgeniy A. ,   Research Center "Kurchatov Institute" – Central Research Institute of KM "Prometey"
Published: October 2023

The efficiency of phytase and impact-disintegrator-activator processing (IDAP) of soybean in obtaining protein hydrolysate from full-fat soybean flour to reduce phytic acid in the hydrolysate was shown. For the experiments, samples of full-fat soybean flour were used, obtained through soybean processing using two different methods: impact disintegration-activation processing (IDAP) and a grinder with a rotor-knife working element (RKP – rotor-knife processing). The IDAP of soybeans was conducted using a DEZI-11 disintegrator unit with six-row rotors rotating at a frequency of 210 s-1. The RKP was carried out using a Gamlux GL-CG mill with a rotor rotation frequency of 20500 rpm. Enzymatic treatment of the mixture of full-fat soybean flour and water (with a water-to-material ratio of 1:6) was performed using the enzyme preparation Phytaflow (with an activity of 20000 U/cm3), with different dosages of phytase added: 20; 40; 60, and 80 U/g of full-fat soybean flour. The enzymatic treatment was conducted at a temperature of 50°C for a duration of 120 minutes. As a result of the studies, it was found that the design of the working body of the chopper affects the degree of soybean degradation, the content of phytic acid in full-fat soybean meal, and the efficiency of phytase action in obtaining protein hydrolysate. The content of phytic acid in full-fat soybean meal obtained after processing on the impact-disintegrator-activator type unit is 12% lower than in full-fat soybean meal obtained after processing on the grinder with a rotary knife working body. Phytase was found to decrease the phytic acid content in protein hydrolysate obtained after processing soybeans with the impact-disintegrator-activator unit more effectively than in protein hydrolysate obtained after processing soybeans with the shredder with the rotary knife-type working body. The hypothesis of the effect of IDAP soybean on the efficiency of phytic acid reduction and enzymatic phytase hydrolysis was confirmed and showed a high degree of significance. The reduction of phytic acid in full-fat soybean meal and protein hydrolysates obtained from it is of great practical importance as it allows to create functional foods with high digestibility and protein bioavailability and to increase the doses of inclusion of full-fat soybean meal in starter feeds of animals, birds, and aquaculture.
Conceptualising Liveness and Visibility in the News Repertoires of Adolescents in a Polymedia Environment
Authors: Jontes Dejan , Črnič Tanja Oblak , Luthar Breda
Published: October 2023

Based on the assumptions that digital media are used as integrated structures or “polymedia repertoires” and that media practices cannot be treated as unrelated practices performed on distinct platforms, the present study examined the digital sociability of young people and their media prosumption in a polymedia environment. Data were collected from group interviews of 67 12- to 19-year-olds and 59 personal visualised media sketches. The study focused on teenage engagement with news as part of their media repertoires and their understanding of what news is in the context of general platform sociability conditions, including a state of permanent connectedness and constant anticipation of something new. Their sociability based on permanent activity and affective engagement was enabled and framed by the algorithmically produced regime of visibility and the promise of liveness. The findings indicated that an important consequence of the increased fragmentation of activities is the naturalisation of the performance of multiple media practices at the same time. Although the complexity of such performance, even among teenagers, revealed socially distinctive categories, clear hierarchies between types of practices—such as watching news or pop culture, online shopping and doing homework—and the cultural differentiation of the dominant contexts for these practices—such as school and leisure—were eroded. The contexts of school, home, and leisure thus collapse, and the definition of important news journalism becomes highly unstable, with the distinction between pop and politics generally disintegrating.
Mechanisms of impaired expression of p53-responsive microRNA genes in diffuse B-large cell lymphoma
Authors: Voropaeva E. N. , Pospelova T. I. , Churkina M. I. , Gurazheva A. A. , Berezina O. V. , Maksimov V. N.
Published: October 2023

Introduction. A more in-depth description of molecular events that disrupt the functioning of the p53 signaling pathway is important for understanding the mechanisms of formation and progression of diffuse B-large cell lymphoma (DCCL), as well as its sensitivity to treatment. The p53 protein exhibits its oncosuppressive function and mediates the antitumor effects of drugs by regulating transcription and/or maturation of a wide range of target genes, including MIR-34A, MIR34B/C, MIR-129-2 and MIR-203. In the tumor tissue of lymphomas, in comparison with normal lymphoid tissue, a decrease in the level of microRNAs encoded by these genes is shown.

Aim. The aim of this study was to conduct a comprehensive analysis of the methylation of the genes of the p53-responsive microRNAs MIR-34A, MIR-34B/C, MIR-203 and MIR-129-2, as well as mutations in the DNA-binding domain and destruction of the polyadenylation signal of the TP53 gene in DLBCL.

Materials and methods. 136 DNA samples isolated from tumor tissue of patients with DLBCL and 11 DNA samples obtained from lymph nodes with reactive B-cell follicular hyperplasia were analyzed. The methylation status of MIR-203 and MIR-129-2 genes was determined by the method of methyl-specific polymerase chain reaction, MIR-34A and MIR-34B/C genes by the method of methyl-sensitive analysis of high-resolution melting curves. In tumor samples, rs78378222 genotyping was performed by polymerase chain reaction with restriction fragment length polymorphism, resulting in the destruction of the polyadenylation signal, and the nucleotide sequence of the region of the TP53 gene encoding the DNA-binding domain was determined by capillary direct sequencing by Sanger.

Results. The methylation detected in lymphoma tissue was tumor-specific. The frequency of analyzed aberrations in the TP53 gene and methylation of MIR-34A, MIR-34B/C, MIR-129-2 and MIR-203 was 21, 23, 55, 65 and 66 %, respectively. At the same time, methylation of the analyzed genes of p53-responsive microRNAs and aberrations in the TP53 gene in the tumor tissue of patients with DLBCL were independent events with a tendency to mutual exclusion. At the same time, it was shown that in the vast majority of lymphoma samples, the methylation of the MIR-34A, MIR-34B/C, MIR-129-2 and MIR-203 genes was combined.

Conclusion. Along with aberrations in TP53, methylation of MIR-34A, MIR-34B/C, MIR-129-2 and MIR-203 genes may be an important cause of decreased expression of miR-34a, miR-34b, miR-34c, miR-129 and miR-203 in DLBCL. The combined methylation of the MIR-203, MIR-129-2 and MIR-34B/C genes, as well as the MIR-34B/C and MIR-34A pairs, potentially has a more pronounced pro-tumor effect due to the presence of common targets in the microRNAs encoded by them.
Expression of the cytoskeletal proteins – cytokeratins and beta-III tubulin in human melanoma cell lines from the collection of N. N. Blokhin National Medical Research Center of Oncology
Authors: Bogush T. A. , Mizaeva I. E. , Basharina A. A. , Grishanina A. N. , Baryshnikova M. A. , Burova O. S. , Rudakova A. A. , Kosorukov V. S.
Published: October 2023

Introduction. Despite advances in the treatment of melanoma, the results of therapy cannot be considered satisfactory, and the search for new drugs and effective combinations of medicine continues. The drugs are being developed aimed at reducing the metastatic tumor potential – migrastatics. The targets of the drugs can be cytoskeletal proteins of tumor cells – cytokeratin (CK) intermediate filaments and microtubule protein beta-III tubulin (TUBB3).

Aim. To estimate of the CK and TUBB3 expression in melanoma cell lines to form an informative in vitro cell model for screening and studying migrastatics.

Materials and methods. The molecular phenotype of 21 human melanoma cell lines from the collection of N. N. Blokhin National Medical Research Center of Oncology, and 18 of which were isolated from tumor metastases in the lymph nodes, soft tissues or subcutaneously. The level of TUBB3 expression and de novo expression of CKs in vimentin-expressing cells (CK + Vim) were assessed by an immunofluorescent method and flow cytometry.

Results. Beta-III tubulin expression was detected in all cultures studied, de novo expression of CKs was found in 20 / 21 lines. The exception was primary uveal melanoma 92-1, that did not express CK + Vim. Both parameters significantly differed between the cells of the studied panel: CK + Vim co-expression – from 0 to 91 %, TUBB3 – from 18 to 86 %. No correlation was found between the expression level of TUBB3 and CK + Vim (Pearson’s correlation coefficient r = 0.11; p = 0.65). Three groups of the cell lines with different ratio of TUBB3 expression and CK + Vim co-expression were identified: 1) similar level of expression of both markers; 2) the level of co-expression of CK + Vim more or less high than the index for TUBB3; 3) the level of TUBB3 expression more or less high than the index for CK + Vim co-expression.

Conclusion. A panel of 21 human melanoma cell lines was formed with quantitatively estimated expression of cytoske-letal proteins responsible for the migration activity of tumor cells – CKs and TUBB3. Groups of the lines with different expression ratio of the markers can be used for screening and preclinical evaluation potential migrastatics that reduce the metastatic potential of melanoma and may reduce resistance to taxanes.
Prognostic fine needle aspiration biopsy of uveal melanoma: Molecular and genetic factors of metastasis risk
Authors: Yarovaya V. A. , Levashov I. A. , Zaretsky A. R. , Chudakova L. V. , Nazarova V. V. , Matyaeva A. D. , Demidov L. V. , Yarovoy A. A.
Published: October 2023

Introduction. Molecular genetic testing is actively used for prognostication in patients with uveal melanoma (UM). Tissue for genetic analysis may be obtained either by surgical excision or through fine-needle aspiration biopsy (FNAB). Performing genetic testing and FNAB in each institution can differ in surgical techniques and laboratory methodologies.

Aim. To present our own experience of performing FNAB-based molecular genetic testing for prognostication in patients with uveal melanoma.

Materials and methods. Prognostic FNAB (n = 151) were combined with brachytherapy or stereotactic surgery. Genetic testing was performed by methods based on polymerase chain reaction (GNAQ, GNA11, EIF1AX and SF3B1 mutations) and fluorescence in situ hybridization (copy numbers of PPARG and MYC genes); cytology of FNAB material was also assessed.

Results. Fine-needle aspiration biopsy material was informative in 91 % of cases. At the median follow-up of 36 months, 12 cases of distant metastases were detected. Occurrence of the assessed mutations and copy numbers were related to other representative studies. PPARG deletion was shown to be a significant prognostic factor for metastasis-free survival (p <0,01), which was demonstrated for the first time; EIF1AX and SF3B1 mutations, MYC amplification and cytological class were not shown to be significantly associated with survival in our study.

Conclusion. FNAB-based molecular genetic testing for prognostication in patients with uveal melanoma was shown to be a reliable and highly informative approach. Some of the prognostic factors need to be evaluated further with longer follow-up.
TRANSFORMATION OF THE CULTURAL LANDSCAPES OF UKRAINIAN CITIES IN THE REAR IN THE CONTEXT OF THE FULL-SCALE WAR
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Stepanenko Anastasiia
Published: October 2023

The War between the Russian Federation and Ukraine is one of the most devastating conflicts in history, with an unprecedented scale in Europe since World War II. It has a profound impact on Ukrainian society, resulting in complex changes on multiple levels, including economic, political, cultural, and social aspects. The cultural landscape of Kyiv undergoes transformations of varying intensity and nature throughout the year 2022. These processes can be caused by changes in the functions of sociocultural institutions and architectural structures, alterations in the names of urban toponyms, the placement of military posts, and defensive structures, as well as shifts in the perception of public space and associations related to the city. It has a significant impact on the identity of the city and its residents, as well as on the perception of events and public activism. Creative communities, artistic associations, and cultural and artistic-oriented civic organizations that continue their activities have adapted their work and become part of an active volunteer movement. This article aims to explore the comprehensive effects of the war on the general trends of cultural landscape transformation in Ukrainian cities. It examines various aspects of how the war has influenced Kyiv, such as its impact on the creative sector, changes in media hierarchy, the transformation of urban cultural practices, and changes in the supply and demand of cultural products and services at the local level. The article attempts to investigate and systematize the processes of cultural landscape transformation in Ukrainian cities that have not been directly involved in active combat during the ongoing war of Russia against Ukraine. By adopting a comprehensive approach, this research seeks to encompass various facets of the war's influence on cities and urban communities and facilitate an understanding of their interconnectedness with the broader trends of cultural landscape transformation
CANCEL CULTURE: ETHICAL AND POLITICAL ASPECTS (IN THE CONTEXT OF THE RUSSIAN–UKRAINIAN WAR)
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Korobko Margaryta , Mishchenko Anna ,   State University of Trade and Economics (Kyiv) Ukraine
Published: October 2023

Today Ukraine is struggling with the war on its own territory. Russian invasion has provoked many discussions in the cultural sphere. In the end of February 2022 Ukraine's Minister of Culture and Information Policy Oleksandr Tkachenko called the world to impose cultural sanctions on the Russian Federation. It means to cancel Russian culture. Cancel culture refers to the popular practice of withdrawing support for (canceling) public figures and companies after they have done or said something considered objectionable or offensive. Cancel culture is generally discussed as being performed on social media in the form of group shaming. What ethical issues this phenomenon implicates? What does it mean for Ukraine? Cancel culture is a complicated phenomenon in the modern world. We can begin our analysis of the contemporary problem of the culture of cancellation in ethics and politics by analyzing the classic works of Hannah Arendt, Isaiah Berlin, and Michael Oakeshott. Oleksandr Klekovkin, a Ukrainian theater expert, actively promotes the idea of abolishing Russian culture in Ukraine. American lawyer Alan Dershowitz sees a negative aspect of the cancel culture phenomenon. So far, a clear understanding of this phenomenon has not been developed academically. The purpose of the article is an attempt to analyze the phenomenon of "cancellation culture" in ethical and political aspects, actualized in the conditions of the Russian-Ukrainian war in the context of the cancellation of Russian culture in Ukraine. If in a certain society, even under the conditions of a democratic political regime, the idea of at least a hypothetical possibility of solving all problems prevails, it will stop at nothing to achieve such an ideal state. This is the lesson we should have learned after two world wars. However, in practice, in many modern societies, there are again attempts to instill value monism, which manifests itself as "cancel culture". By legitimizing "cancel culture" as a modern trend, political systems significantly limit discursiveness and pluralism within society. After all, it becomes too uncomfortable to express an opinion that contradicts the generally accepted one. This threatens career, social status, and sometimes even safety.
Bacterial vaginosis: modern aspects of pathogenesis, diagnosis, and treatment
Authors: Dobrokhotova Yulia E. , Kazantseva Valeriya D. , Ozolinya Lyudmila A.
Published: October 2023

In healthy women under normal conditions, the vaginal microbiome is primarily dominated by Lactobacillus (lactobacilli), which produce a variety of antimicrobial compounds. Bacterial vaginosis (BV) is characterized by the absence or significant decrease in the total number of lactobacilli and an increase in the concentration of anaerobic microbes. It is a prevalent condition among women of reproductive age worldwide. Its influence on adverse gynecological and obstetric outcomes, such as sexually transmitted infections, pelvic inflammatory diseases, and preterm labor, has been confirmed. Gardnerella vaginalis is the most frequent microorganism identified in BV and predominant in polymicrobial biofilms, which serve as a protective barrier for G. vaginalis and other BV-associated microbes from the host organism.
This review discusses the latest research findings on the pathogenesis of BV, diagnostic approaches, treatment options, and possible strategies to reduce disease recurrence.
Although various treatment regimens for the disease are currently known, they often lack long-term effectiveness. The aim of this review is to consider the peculiarities of the vaginal microbiome, summarize the current information on the development and methods of treatment of BV, and emphasize on the latest achievements of conducted studies.
Predictive calculator of the risk of perinatal complications in women with pregestational diabetes mellitus
Authors: Dudareva Yuliya  A. , Seroshtanova Daria N. , Dronov Sergei  V. , Antoshkina Larisa  V.
Published: October 2023

Background. The prevalence of impaired carbohydrate metabolism among women of reproductive age is increasing worldwide. Despite tremendous progress in the treatment and management of blood glucose levels, pregnancy in women with pregestational diabetes still carries risks for the fetus.
This study aims to develop a calculator for predicting perinatal complications in women with pregestational diabetes mellitus by mathematical modeling.
Materials and Methods. This observational analytical study with a case-control design was conducted at the Altai Regional Clinical Perinatal Center DAR (Barnaul). The study included 147 women, with the main group comprising 95 pregnant women, including 47 with type 1 diabetes mellitus (group 1A) and 48 with type 2 diabetes mellitus (group 1B). No carbohydrate metabolism disorders were detected in 52 patients of the control group. All patients in the main group received insulin therapy. Medical documentation was analyzed, and statistical processing of the data was performed using mathematical modeling methods with appropriate software.
Results. In order to predict the combined indicator of perinatal complications, logistic regression analysis was used to calculate coefficients (b) for each of the indicators that have the most significant influence on the formation of complications.
The calculated values of regression coefficients can be utilized to predict the risk of perinatal complications in women with type 1 diabetes mellitus. For more practical use, a calculator for assessing the risk of perinatal complications in type 1 and type 2 diabetes mellitus was created using a computer program.
Diagnostic evaluation of the prognostic scale (calculator) for assessing perinatal complications risk assessment in type 2 diabetes mellitus demonstrated a sensitivity of 97.6%, specificity of 87.5%, and a prognostic value of positive risk assessment of 97.5%. Therefore, the calculator enables the prediction of the risk of perinatal complications in 97.5% of cases. At the same time, the prognostic scale of perinatal complications risk and the Perinatal Complications Risk Calculator for type 1 diabetes mellitus created on its basis showed 100% sensitivity and specificity.
Conclusion. The frequency of perinatal complications remains high, so the creation of a sufficiently effective prognostic model will make it possible to predict perinatal complications and influence the tactics of management of pregnant women and their newborns.
Clinical cases of childbirth in 13-year-old adolescents
Authors: Grigoryan Narinе V. , Sinchikhin Sergey P. , Stepanyan Lusine V.
Published: October 2023

In the literature, limited articles have been found regarding childbirth in minors, with the majority of young women giving birth falling between ages of 15 and 18. Therefore, the authors found it noteworthy to present two clinical cases of childbirth in 13-year-old adolescents. Both girls come from single-parent families, are not enrolled in education or employment, and had unwanted pregnancies. They did not receive prenatal care from a gynecologist or register at an antenatal clinic. During their pregnancies, both girls experienced various diseases and complications. This article highlights that pregnancy and childbirth during adolescence are often accompanied by obstetric complications, such as hypotonic bleeding, premature detachment of the normally located placenta, preeclampsia, and iron deficiency anemia. Both underage mothers left the hospital, leaving their newborns (abandoned the child). The authors emphasize the importance of preventing unwanted pregnancies among minors and providing supervision by psychologists and state social services to support young mothers.
Kibar Feyzo ve Organize İşler Filmlerinde Merkezi İktidar ile Çevrenin Toplumsal Analizi
Authors: ÇANKAYA Koray
Published: October 2023

Modern dönemde iktidar pratikleri paternalist bir bakış açısıyla merkez-çevre ilişkisi içerisinde kendisini yapılandırmıştır. Bu iktidar pratiği kapatma ve dışlama rejimleri üzerinden işlerlik kazanmaktadır. Postmodern dönemle birlikte ise iktidarın toplumsal alandaki parçalanmasının bir sonucu olarak sosyo-kültürel alanın dokusuna nüfuz etmiş mikro iktidar pratiklerinden bahsetmek mümkün hale gelmiştir. Bu bağlamda Türk sinemasında Kibar Feyzo ve Organize İşler filmleri ele alındığında iktidarın iki ayrı örgütlenme şekli bu filmlerde somutlaşmaktadır. İlkinde merkezi iktidar pratiklerinin ve egemen diskurun söylemsel pratikleri öne çıkarken ikinci filmde ise mikro düzeyde parçalı bir iktidar pratiği göze çarpmaktadır. Toplumsal dönüşüm sürecinde iki filme bakıldığında geleneksel toplumsal ilişkilerden ve iktidar pratiklerinden modern döneme geçişi niteleyen Kibar Feyzo filmi feodal üretim ilişkileri içerisinde özgürleşmenin ve var olan disiplin sisteminin kırılması yönünde mesajları izleyicilere taşımaktadır. Oysa Organize İşler filmi merkezi iktidarı niteleyen ulus devlet mekanizması ve neoliberal dönemde denetim sistemlerinin aşındığı kentsel yaşam formlarındaki kaotik varoluş mücadelesi içerisindeki akışkan iktidar pratiklerini içermektedir. Bu çalışmada modern dönemden postmodern döneme Türk filmlerinde toplumsal yapının politik örgütlenmesi üzerinden kültürel dönüşümü incelenmektedir. Çalışmanın amacı Türk toplumunun kolektif modern pratikler ve kültür yapısının postmodern bireyci yapıya dönüşümünü, postmodern kuramsal perspektiften analiz etmektir. Bu bağlamda 1978 ve 2005 yılı kapsamında amaçlı örneklem tekniği ile seçilen Kibar Feyzo ve Organize İşler filmleri niteliksel içerik analizi tekniğiyle alana ilişkin kuramsal perspektif temelinde oluşturulan kategoriler üzerinden çözümlenmiştir.