bluuebunny/crossref_metadata_2025 huggingface dataset:
Authors: Prakosa Aditya Priagung , Aliyyu Wienda Cinta , Rosyadah Nada Janardita , Nur Saniyya , Amalia Rahma Ika
Published: September 2023

Diabetes mellitus is a chronic non-communicable disease caused by metabolic disorders in the pancreas organ characterized by an increase in blood sugar levels beyond normal. The Southeast Asian region, which includes Indonesia, is ranked 3rd in the world with a prevalence of diabetes mellitus reaching 11.3%. Meanwhile, Indonesia is ranked 7th out of 10 countries with the most diabetes mellitus sufferers, reaching 10.7 people. Diabetes mellitus can manifest into other diseases, one of which is abnormalities in the oral cavity. Common manifestations of diabetes mellitus in the oral cavity include Oral Infection (Candidiasis), Xerostomia, Periodontal Disease, Recurrent Dental Caries, Burning mouth sensation (related to xerostomia), Taste Dysfunction, Oral Mucosa Alteration (Lichen Planus), Poor Oral Wound Healing. This literature review describes the systemic disease of diabetes mellitus, diagnostic investigations and management of oral manifestations of diabetes mellitus. Management of oral manifestations of diabetes mellitus does not only focus on treating diseases in the oral cavity, but it is more important to eliminate etiological factors and avoid predisposing factors.
Biological Assessment of Anthropogenic Impacts in Buguma Creek, Rivers State, Nigeria
Authors: Chris Davies Ibienebo , Anyanwu Emeka Donald
Published: September 2023

The objective of this research was to assess the anthropogenic impact on plankton and macrobenthic fauna composition, abundance, distribution, and diversity of four communities in Buguma creek. The Phytoplankton, zooplankton, and benthic fauna samples were collected quantitatively monthly from each of the four sampling stations between January and June 2020 using standard sampling methods. Margalef (D), Shannon Wienner (H), and Evenness indices were used to determine species richness and diversity respectively using the PAST statistical package. This study revealed that artisanal refinery activities, sand mining/dredging, and discharge of industrial, domestic, human, and animal wastes have adversely affected the aquatic biota (plankton and macrobenthic fauna) in Buguma creek. The effects of these activities have remarkable spatial manifestations; with the more perturbed especially station 2, having a lower number of species and abundance. The preponderance of indicator species is a confirmation while the community structure gave an insight into the negative impact of these activities individually and cumulatively. The brunt of these activities rests more on the macrobenthic fauna; probably due to their unique characteristics and position in the aquatic environment. The result indicated that Buguma creek had been polluted seriously to a large extent. Keywords: Aquatic biota, Bioindicator, Anthropogenic, Artisanal refinery, Diversity
Sediment Characteristic of the Ebb-Tidal Delta in Western Segara Anakan Lagoon
Authors: Cahyo Tri Nur , Hartoko Agus , Muskananfola Max Rudolf , Haeruddin Haeruddin
Published: September 2023

Western Segara Anakan Lagoon (WSAL) is a semi-enclosed lagoon located in the Cilacap, Central Java Province, Indonesia which has two outlets called pelawangan. West Pelawangan is an open channel from WSAL and the Indian Ocean, which has vast sediment input. It impacts the sediment deposits and delta formations in WSAL. This research aimed to determine the sediment characteristics deposited in the WSAL specifically in the delta formation area, and the mean size of sediments (D50), sorting, and skewness. The research used the survey method while data collected were sediment-bed, bathymetry, and satellite imagery Sentinel 2A with the ebb-tide condition. Sediment was analyzed by sieve and gravimetry and interpreted as a Ternary diagram, D50, sortation, skewness, and fraction percentage spreading map. Satellite imagery Sentinel 2A was searched by the ebb-tide condition. Shorelines and delta was extracted by the Normalized Different Water Index. Bathymetry built from depth points data 2018. The results of the Ternary diagrams analysis were the Citanduy River had a sand grain size, the Pelawangan Barat Waters (PBW) were dominated by sand and the lagoon was dominated by a fine grain size and silt. The result of sediment D50, sortation, and skewness in the Citanduy River and the PBW were dominated by sand, and the lagoon was dominated by fine sediment: silt, and clay grain size. The sediment fractions were sorted very well; skewness was dominated by the fine skewed (positive skewed). The delta formation in the lagoon covers a large area, presumably due to the consolidation of the fine sediment.Keywords: Sediment Characteristics, Ebb-Tidal Delta, Western Segara Anakan Lagoon
Neural Coupling between Interhemispheric and Frontoparietal Functional Connectivity during Semantic Processing
Authors: Soshi Takahiro
Published: September 2023

Interhemispheric and frontoparietal functional connectivity have been reported to increase during explicit information processing. However, it is unclear how and when interhemispheric and frontoparietal functional connectivity interact during explicit semantic processing. Here, we tested the neural coupling hypothesis that explicit semantic processing promotes neural activity in the nondominant right hemispheric areas owing to synchronization with enhanced frontoparietal functional connectivity at later processing stages. We analyzed electroencephalogram data obtained using a semantic priming paradigm, which comprised visual priming and target words successively presented under direct or indirect attention to semantic association. Scalp potential analysis demonstrated that the explicit processing of congruent targets reduced negative event-related potentials, as previously reported. Current source density analysis showed that explicit semantic processing activated the right temporal area during later temporal intervals. Sub-sequent dynamic functional connectivity analyses of current source density revealed that explicit semantic processing increased the correlation between right temporal source activities and frontoparietal functional connectivity in later temporal intervals. These findings indicate that explicit semantic processing increases neural coupling between the interhemispheric and frontoparietal functional connectivity during later stages of semantic processing.
On the Taxonomy of Chigger Mites (Acariformes: Trombiculidae) Parasitizing Birds in Thailand and Malaysia, with the Description of a New Species
Authors: Koosakulnirand Sirikamon , Rajasegaran Praveena , Alkathiry Hadil , Chaisiri Kittipong , Round Philip , Eiamampai Krairat , Ahmad Khusaini Mohd , Fizl Sidq Ramij Mohamad , Abubakar Sazaly , Ya'cob Zubaidah , Stekolnikov Alexandr , Makepeace Benjamin
Published: September 2023

Chigger mites were collected from 65 bird species in different regions of Thailand and Malaysia. In total, 21 species were recorded. The previously unknown fauna of chiggers parasitizing shorebirds of Thailand included one new species, Neacariscus (Whartonacarus) ... n. sp. as well as Neacariscus (Neacariscus) pluvius (Wharton, 1945) previously recorded only in Oceania; Neacariscus (Whartonacarus) shiraii (Sasa, Kano & Obata, 1952) (known from Oceania and Japan); and Neacariscus (Whartonacarus) sulae (Oudemans, 1910) and Schoengastia archaea (Taufflieb, 1960), both described from West Africa. Toritrombicula kirhocephales Goff, 1982 described from Papua New Guinea was for the first time recorded in Asia (Thailand). Neoschoengastia gallinarum (Hatori, 1920) and Helenicula comata (Womersley, 1952) were for the first time recorded in Thailand. Ascoschoengastia lorius (Gunther, 1939) was for the first time recorded in Malaysia. Finally, Ericotrombidium cosmetopode (Vercammen-Grandjean & Langston, 1971) described from free larvae, was for the first time recorded on a host (bird Pellorneum ruficeps Swainson); and Leptotrombidium miculum (Traub & Audy, 1954) was for the first time recorded on a bird host. New host species were recorded for the large part of the collected chigger species.
Observational Evidence for that Newtonian Theory of Orbit Perturbation Is Fundamental to Galactic System
Authors: zhu yin
Published: September 2023

In recent, Chae found that, at weak gravitational acceleration gN≲10−9ms−2, the observed orbit of the wide binary stars is not accordant with current theory of binary star. Here, we present, this finding should be an observational evidence for that Newtonian theory of orbit perturbation is still valid in galactic scale.
Incidence of the Risk of Malnutrition and Excess Fat Mass, Low Total Lean Tissue, and Gait Speed as Independent Associated Factors in Community-Dwelling Older Adults
Authors: López Teros Miriam Teresa , Vidaña-Espinoza Helen Joceline , Esparza-Romero Julián , Rosas-Carrasco Oscar , Luna-López Armando , Alemán-Mateo Heliodoro
Published: September 2023

Background and aims: Only one cohort study exists on the incidence of the risk of malnutrition (RM) in older adults, though numerous cross-sectional –and that cohort research– have reported several risk factors associated with the prevalence and incidence of this condition. However, alterations of body composition and impaired physical performance as exposition variables of RM have not been explored. This study assessed the incidence of RM and determined its association with excess fat mass, low total lean tissue, gait speed, and handgrip strength as exposition variables for RM in community-dwelling older adults.
Methods: This is a secondary analysis in older adults (≥60 years old) derived from the study “Frailty, dynapenia, and sarcopenia in Mexican adults (FraDySMex)” a prospective cohort project conducted from 2014 to 2019 in Mexico City. At baseline, volunteers underwent body composition analysis and physical performance tests. Several covariates were identified through comprehensive geriatric assessment. At baseline and follow-up, RM was assessed using the long form of the mini nutritional assessment (MNA-LF) scale. Associations between the exposition variables and RM were assessed by multiple logistic regression.
Results: The cohort included 241 subjects. Average age was 75.6±7.8 years; 83.4% were women. The mean follow-up period was 4.1 years, during which 28.6% of subjects developed RM. This condition was less likely to occur in subjects with excess fat mass index, even after adjusting for several covariates. RM was also more likely to occur in subjects with low total lean tissue index, according to both an unadjusted model and after adjusting for the corresponding variables. Regarding the association between RM and gait speed, RM was also more likely to occur in subjects with low gait speed according to both the unadjusted and adjusted models. Similar results were found for RM in relation to low handgrip strength; however, after adjusting for the associated covariates, models 1 and 2 no longer reached the level of significance.
Conclusion: The risk of malnutrition diagnosed by MNA-LF was significantly less likely to occur among subjects with excess fat mass, but possibility was significantly higher in those with low total lean tissue and low gait speed after 4.1 years of follow-up in community-dwelling older adults. These results highlight that excess fat mass, low total lean tissue, and low gait speed are first than risk of malnutrition and not vice versa.
Overlapping Neuroimmune Mechanisms and Therapeutic Targets in Neurodegenerative Disorders
Authors: De Marchi Fabiola , Vidatic Lea , Papić Eliša , Rački Valentino , Nimac Jerneja , Jurak Igor , Rogelj Boris , Vuletic Vladimira , Liscic Rajka M. , Cannon Jason R. , Buratti Emanuele , Mazzini Letizia , Munitic Ivana , Hecimovic Silva
Published: September 2023

Many of the potential immune therapeutic targets are similarly affected in adult-onset neurodegenerative diseases such as Alzheimer’s (AD) disease, Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia (FTD), but also in a seemingly distinct Niemann-Pick type C disease with primarily juvenile-onset. This strongly argues for an overlap in pathogenic mechanisms. The commonly researched immune targets include various immune cell subsets such as microglia, peripheral macrophages, or regulatory T cells (Tregs), the complement system, and other soluble factors. In this review, we will compare these neurodegenerative diseases from a clinical point of view and point out the common pathways and mechanisms of protein aggregation, neurodegeneration and/or neuroinflammation that could potentially lead to shared treatment strategies. We also describe the common therapeutic approaches in treating the immune dysfunctions in these disorders, moving from immunization to microbiome regulation and stem cell treatment.
Vegetation Analysis of the Area Surrounding a Wild Nest of Stingless Bees (Tetragonula laeviceps, Smith 1857) in Sumedang Regency, West Java
Authors: Withaningsih Susanti , Lubay Valerie , Rozi Fakhrur , Parikesit Parikesit
Published: September 2023

Indonesia is a mega biodiversity country that has a very abundant diversity of plants. Plant diversity is inseparable from the role of insects that help to pollinate them. One such insect is the stingless bee (Tetragonula laeviceps). Tetragonula laeviceps is a bee that has a relatively small-to-medium body size and does not have a sting. Sumedang Regency is one of the regencies in the province of West Java; most of the Sumedang area consists of mountains and has protected forest areas with high biodiversity. The purpose of this study is to determine the biodiversity index of the vegetation around the Tetragonula laeviceps wild hives based on the composition and structure of the vegetation around the Tetragonula laeviceps bee hives. Data collection is carried out using the method of inventory and systematic checkered lines, with a total plot of 320. The results of the study show that the vegetation is composed of 229 plant species from 75 families. The most dominant vegetation type with the highest value of KR, FR, DR, and INP in all plant categories is the Aren tree species (Arenga pinnata). The Shannon–Wiener diversity index in this tree category is 3,186, the pole category is 3,107, the sapling category is 3,418, the seedling category is 3,657, and the understory plants category is 3,409, with all of the plant categories included in the high-diversity category.
Constitutive Modeling of Annealed OFHC with Wide Strain-Rate and Temperature Effects: Incorporating Dislocation Dynamics and Normalized Microstructural Size Evolution
Authors: Xu Mengwen , Xiao Qiangqiang , Zu Xudong , Tan Yaping , Huang Zhengxiang
Published: September 2023

The flow stress of face-centered cubic (FCC) metals exhibits a rapid increase near a strain rate of 104 s-1 under fixed strain conditions. However, many existing constitutive models either fail to capture the mechanical characteristics of this plastic deformation or use piecewise strain rate hardening models to describe this phenomenon. Unfortunately, the piecewise models may suffer from issues such as discontinuity of physical quantities and difficulties in determining segment markers, and struggling to reflect the underlying physical mechanisms that give rise to this mutation phenomenon. In light of this, this paper proposes that the abrupt change in flow stress sensitivity to strain rate in FCC metals can be attributed to microstructural evolution characteristics. To address this, a continuous semi-empirical physical constitutive model for FCC metals is established based on the microstructural size evolution proposed by Molinari-Ravichandran and the dislocation motion slip mechanism. This model effectively describes the mutation behavior of strain rate sensitivity under fixed strain, particularly evident in the annealed OFHC. The predicted results of the model across a wide range of strain rates (10-4 − 106 s-1) and temperatures (77 − 1096 K) demonstrate relative errors generally within ±10% of the experimental values. Furthermore, the model is compared to five other models, including Mechanical Threshold Stress (MTS), Nemat-Nasser-Li (NNL), Preston-Tonks-Wallace (PTW), Johnson-Cook (JC), and Molinari-Ravichandran (MR) models. A comprehensive illustration of errors reveals that the proposed model outperforms the other five models in describing the plastic deformation behavior of OFHC. The error results offer valuable insights for selecting appropriate models for engineering applications and provide significant contributions to the field.
Micro- and Macroplastics Pollution in the Aquatic Environment of Markakol Lake Located in the Protected Area on the Mountain Slopes of the Southern Part of the Kazakh Altai Mountains
Authors: Madibekov Azamat , Ismukhanova Laura , Sultanbekova Botakoz , Zhadi Askhat , Zhumatayev Serik , Musakulkyzy Ainur , Bolatov Kainar , Madibekova Aisha
Published: September 2023

Abstract. The paper presents primary material on determining the presence of micro- and macroplastics in Markakol Lake, one of the high-mountainous and unique water bodies of Kazakhstan, conditionally undisturbed, but vulnerable to anthropogenic pollution. In the course of work, micro- and macroplastics were detected in all selected samples in the water area of the lake and its main tributaries, the Urunkhaika, Topolevka, Tikhushka, Matabai, Elovka rivers. When determining micro- and macroplastics, we were guided by the method of analysis in aquatic environment, NOAA research program, developed by American scientists Masura J. et al, which is used in many countries. Concentrations and sizes of MP found in the water of the main tributaries of the rivers Urunhaika in concentrations up to 211.4 µg/m3, Tikhushka – 97.9 µg/m3, Zhirelka – 67.8 µg/m3, Topolevka – 157.2 µg/m3, Matabai – 78.3 µg/m3. Concentrations of MP in the surface water layer of the lake detected during 15 minute trawling at distances from 438 m to 841 m occur in sieve mesh sizes of 1.0 mm – 316.7 µg/m3 and 0.315 mm – 520.8 µg/m3. The total concentration of detected micro- and macroplastics coming with tributaries was – 150 µg/m3, in the lake water area – 837.4 µg/m3. The sizes of plastic debris found both in river waters and along the lake water area ranged from mesoplastic debris (fishing line net fragments, foam balls, plastic bags, plastic bottles, wrappers, food labels and packaging, etc.) to microplastic particles detected using a microscope with 40X magnification in size ranges of 25 mm, 1.0 mm and 0.315 mm. Sources of MP depend on local human activities (fishing, transportation, waste disposal etc.).
Role of SH3PXD2B in Breast Cancer Lung Metastasis through Computational Structural and Systems Perspective
Authors: Das Subhajit , Kharat Komal , Singh Shailza
Published: September 2023

Breast Cancer metastasis remains a formidable challenge in cancer research, contributing significantly to patient mortality despite advances in medical research. Lung metastasis, associated with breast cancer, poses an ongoing clinical dilemma with limited curative treatment options. This study delves into the intricate mechanisms underlying Breast Cancer-Lung Metastasis (BC-LM) primarily focusing on the function of SH3PXD2B in maturation of invadopodia, inducing epithelial-to-mesenchymal transition, disruption of proteostasis network, and ultimately leading to metastasis of Breast Cancer (BC) cells. With an extensive analysis of differential gene expression using RNASeq data, comparing normal breast cancer cells to metastatic sub-populations in lung. Employing the New Tuxedo pipeline our investigation notably observes SH3PXD2B as a key regulator in lung metastasis samples. This trend is further substantiated by data from the Cancer Cell Line Encyclopedia (CCLE), and Human Protein Atlas (HPA) which highlights elevated SH3PXD2B expression in MDA-MB-468 cells, underscoring its significance in metastatic adenocarcinoma. Additionally, we checked the overall survival (OS) of metastatic breast cancer (MBC) patients pinpointing SH3PXD2B and its associated partners like SH3PXD2A, MMPs, CTTN, ADAMs, and EMT markers with substantial expression in both BC and lung cancer, prognosticating poorer patient survival. Further, our transcription factor – target gene (TFTG) network essentially elucidated the role of SH3PXD2B as a key node in the network unveiling its roles in regulating cell migration, communication, and developmental processes. Proteomics and Western blotting assays consistently confirm heightened SH3PXD2B expression in BC cell lines, reaffirming our findings. By employing computational structure biology along with cancer systems biology approach, we generated a high-confidence structural model of SH3PXD2B, indicating its SH3_2 and SH3_3 domains crucial for interactions with the drug molecules. Molecular docking simulations identify Eribulin as a promising therapeutic agent capable of targeting these domains. Thus, our multidisciplinary approach seamlessly amalgamates systems medicine principles, aiming to repurpose existing drugs that target SH3PXD2B based on molecular signatures. Targeted therapies have emerged as a promising avenue for addressing MBC and this mechanistic model introduces novel therapeutic avenues for the treatment of BC-LM patients.
Osteology of the Hamadryas Baboon (Papio hamadryas)
Authors: Casteleyn Christophe , Wydooghe Estée , Bakker Jaco
Published: September 2023

Besides living as a free-ranging primate in the horn of Africa and the Arabian Peninsula, the hamadryas baboon has an important place in zoos and can be found in biomedical research centers worldwide. To be valuable as a non-human primate laboratory model for man, its anatomy should be portrayed in detail allowing for the correct interpretation and translation of obtained research results. Reviewing the literature on the use of the baboon in biomedical research revealed that very limited anatomical works on this species are available. Anatomical atlases are incomplete, use archaic nomenclature and fail to provide high definition color photographs. Therefore, the skeletons of two male hamadryas baboons were prepared by manually removing as much soft tissues as possible followed by maceration in warm water to which enzyme-containing washing powder was added. The bones were bleached with hydrogen peroxide and degreased by means of methylene chloride. Photographs of the various bones were taken, and the anatomical structures were identified using the latest version of the Nomina Anatomica Veterinaria. As such, the present article shows 31 annotated multipanel figures. The skeleton of the hamadryas baboon generally parallels the human skeleton, but some remarkable differences have been noticed. If these are taken into consideration when evaluating the results of experiments using the hamadryas baboon, justified conclusions can be drawn.
Clinical Applications of Combined İmmunotherapy Approaches in Gastrointestinal Cancer: A Case-Based Review
Authors: Eralp Yesim , Ates Utku
Published: September 2023

Malignant neoplasms arising from the gastrointestinal (GI) tract are among the most common cancer types with a high mortality rate. Despite advances in treatment in a small subgroup harboring targetable mutations, the outcome remains poor, accounting for one in three cancer-related deaths observed globally. As a promising therapeutic option in various tumor types, immunotherapy with immune checkpoint inhibitors has also been evaluated in GI cancer, albeit with limited efficacy except for a small subgroup expressing microsatellite instability. In the quest for more effective treatment options, energetic efforts have been placed to evaluate the role of several immunotherapy approaches comprising of cancer vaccines, adoptive cell therapies and immune checkpoint inhibitors. In this review, we report our experience with personalized dendritic cell cancer vaccine and cytokine-induced killer cell therapy in three patients with GI cancers and summarize current clinical data on combined immunotherapy strategies.
Validation of the Histological Diagnosis of Hydatidiform Moles with p57KIP2 Immunophenotyping at the University Teaching Hospitals of Kigali and Butare (CHUK, CHUB)
Authors: Thomas Habanabakize , David Ntirushwa , Felix Manirakiza , Annette Uwineza , Belson Rugwizangoga
Published: September 2023

Background: The hydatidiform moles remain prevalent in spectrum of gesta-tional trophoblastic diseases (GTDs). In resource-limited settings like Rwanda, the definitive diagnosis relies upon single of histomophological diagnosis. The histomorphology alone suffers from high interobserver and intra-observer vari-ability with poor diagnostic reproducibility. The present study aimed at deter-mining the role of p57 immunophenotyping in the validation of histomorpholog-ical diagnosis of hydatidiform moles.
Methods: A retrospective cross-sectional study embarked for histological cases collected between January 2017 and June 2020. A review of Hematoxylin &Eosin(H&E) stained slides was performed with subsequent p57 immunohisto-chemical staining.
Results: Recorded were 211 retrospective cases of hydatidiform moles and 96 (45.9%) cases were all subjected to p57 immunohistochemical staining consid-ered as gold standard diagnostic modality in the present study. As result, the sensitivity and specificity of the histomophological diagnosis of complete hyda-tidiform mole were estimated at 62.5% and 57.1% respectively with positive and negative likelihood ratio of 0.145 and of 0.54 respectively. PPV and NPV were 81.8% and 29.3%, respectively. Whereas of 57.1% and 79.2% with PPV and NPV of 42.9% and 83.8% respectively for partial hydatidiform moles. Per the Youden J statistics method, the accuracy estimation of histomophological diagnosis of hydatidiform mole (HM) was 0.196 (CHM) and 0.336(PHM).
Conclusions: This study highlighted a need to integrate p57 immunostaining in routine histopathological diagnosis of hydatidiform moles refining the defini-tive diagnosis.
Computational Fluid Dynamics Modeling of the Filtration of 2D Materials Using Hollow Fiber Membranes
Authors: Elahi Arash , Chaudhuri Santanu
Published: September 2023

The current study presents a computational fluid dynamics model designed to simulate the isolation of 2D graphene components from their dispersion using a microfiltration hollow fiber membrane unit. The model utilizes Navier-Stokes and mass conservation equations to predict the flow and concentration behaviors in the system, and it employs the resistance-in-series approach to couple the fouling of the non-exfoliated graphites in the dispersion to the model. Specifically, the model accounts for the effects of the concentration polarization and cake formation of the fouling component, resulting in the increment of the transmembrane pressure (TMP) of the system. Additionally, we assumed the wettability of polymeric membrane’s inner wall enhances upon fouling by the flat layered structure material, leading to the reduction of the transmemrabe pressure. This approach demosntrates accurate reproduction of the exceptional experimental TMP behavior corresponding to the fouling of the graphite.
On-site evaluation of constituent content and functionality of Perilla frutescens var. crispa using fluorescence spectra
Authors: Sano Hidemichi , Kawaguchi Satoru , Iimori Toshifumi , Kuragano Masahiro , Tokuraku Kiyotaka , Uwai And Koji
Published: September 2023

Perilla frutescens leaves are expected to exhibit antioxidant activity and amyloid-β (Aβ) aggregation inhibitory activity due to the presence of polyphenol-type compounds, etc. These activities fluctuate daily; however, measuring the contents of constituents and functionalities is cumbersome and unsuitable for instantaneous measurements in the field. In this study, we developed a facile method to analyze the activity and content of the components in leaves on-site based on the fluorescence spectra obtained by irradiating the perilla leaves with excitation light. The constituent contents (Chlorophyl (Chl), Total polyphenol content (TPC), Total flavonoid content (TFC), rosmarinic acid (RA) and functional properties DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), Aβ aggregation inhibitory activity) were evaluated using established analytical techniques. The correlation between the fluorescence spectrum and the contents and activities was calculated using normalized difference spectral index (NDSI), ratio spectral index (RSI), and difference spectral index (DSI) analyses. We identified a predictive model with a high coefficient of determination R² ≥ 0.57 for content of the constituents, and R² ≥ 0.49 for functional properties. This allows convenient, simultaneous, and non-destructive monitoring of the constituent contents and the functionalities of perilla leave and a simple harvest-time diagnosis. The model established using this method is simple and suggesting that it can simultaneously infer the constituents and functionalities of perilla leaves.
Modulation of Motor Awareness: A Transcranial Magnetic Stimulation Study in the Healthy Brain
Authors: Salatino Adriana , Sarasso Pietro , Piedimonte Alessandro , Garbarini Francesca , Ricci Raffaella , Berti Anna
Published: September 2023

Previous studies on the mechanisms underlying willed actions reported that the premotor cortex may be involved in the construction of motor awareness. However, its exact role is still under investigation. Here we investigated the role of the dorsal premotor cortex (PMd) in motor awareness by modulating its activity applying inhibitory rTMS to PMd, before a specific motor awareness task (under three conditions: without stimulation, after rTMS and after sham stimulation). During the task, subjects had to trace straight lines to a given target, receiving a visual feedback of the line trajectories on a computer screen. Crucially, in most trials the trajectories on the screen were deviated and to produce straight lines, subjects had to correct their movements towards the opposite direction. After each trial, participants were asked to judge whether the line seen on the computer screen corresponded to the line actually drawn. Results show that participants in the no stimulation condition did not recognize the perturbation until 14 degrees of deviation. Importantly, active, but not sham, rTMS significantly modulated motor awareness, decreasing the amplitude of the angle at which participants became aware of the trajectory correction. These results suggest that PMd plays a crucial role in action self- monitoring.
Effects of Melatonin Supplementation on Professional Football Player Performance: A Systematic Review
Authors: Almendros Antonio Jesus , Lopez-Moro Alejandro , Conde-Pipo Javier , Santalla Alfredo , Requena Bernardo , Mariscal-Arcas Miguel
Published: September 2023

Background: to know the existing documentation related to exogenous melatonin in the performance of professional soccer players. Methods: critical and systematic review. Data were obtained by looking up the following bibliographic data bases: Web of Science, MEDLINE (via PubMed), Embase, Cochrane Library and Scopus. The used terms were "Soccer Athlete", "Melatonin" and "Soccer Performance", using as a filter: "Humans". The search update was in May 2023. Results: having applied the inclusion and exclusion criteria, 8 articles were selected out of 59 retrieved references. The dose of melatonin administered in the studies ranged between 5 and 8 mg. The outcomes showed a decrease in oxidative stress, muscle damage and inflammatory markers in the melatonin-treated group. Conclusions: Exogenously administered melatonin seems to attenuate some of the effects derived from physical exercise such as oxidative stress, inflammation and muscle damage in professional soccer players, and since it has no potential adverse effects, it could be interesting to apply it in this population. However, the direct effects of melatonin supplementation on physical performance have not been demonstrated, so more research is needed on the intervention period, effective dose and larger participant populations.
The Environmental Performance of Zero Emission Buildings in a Fossil-Free Energy System
Authors: Jonasson Jonas , Faith-Ell Charlotta , Carlman Inga , Englund Oskar
Published: September 2023

Buildings can play an important role in reducing GHG emissions through increased energy efficiency. The European Commission issued a proposal in 2021 suggesting that all new buildings should be “zero-emission Buildings” (ZEB), aiming at a zero GHG emission building stock by 2050.The extent to which ZEB can contribute to reduced GHG emissions, however, varies between countries, due to different energy systems. It is also important to consider other environmental effects to avoid that climate benefits come with unintended consequences.Here, we explore the life-cycle environmental performance for a ZEB in a case where electricity and heating is largely fossil-free. The assessment concentrates on i) environmental impact of the use stage in relation to the product stage, ii) the interrelation between different energy sources, with attention on household electricity, and iii) the performance for more impact categories than primary energy use and climate change. While our results generally support the use of ZEBs from an environmental perspective, they also show that the climate benefit in this setting is marginal. However, given that energy systems are connected and energy savings in one place can reduce the demand for fossil energy elsewhere, the climate benefit of ZEBs is likely underestimated. Besides methdological implications for future studies, this indicates that current EU policy is promising, as incentives for implementation of ZEBs is unaffected by domestic effects.
The Impact of Testicular Hyperthermia, Its Physiological Relevance and the Ability of Antioxidants to Overcome the Condition
Authors: Robinson Ben J , Ogle Rachel A , Netherton Jacob J , Villaverde Ana Izabel , Gunn Allan , Baker Mark
Published: September 2023

Testicular heat stress is a well described phenomenon that occurs in mammals that possess a scrotum. Different models to induce testicular hyperthermia, such as surgical cryptorchidism, hot water bath, scrotal insulation or increased environmental temperature have all shown that spermatocytes and spermatids are unambiguously affected by high temperature, resulting in poor sperm production weeks later. Furthermore, a body of evidence suggest the involvement of oxidative stress is either a major or contributory pathway, which gives rise to the potential to overcome this condition. Whilst experimental models conclusively show the deleterious effect of testicular heat on sperm quality, the physiological relevance of the work is still debated. Herein we summarise a cohort of studies that report the effect of “season” on sperm quality. The data show season can affect sperm production, motility and morphology depending on where the work was performed. In countries where temperatures drop below zero, there is evidence showing summer conditions tend to improve semen quality. However, in sub-tropical countries, some studies show a decrease in summer, whilst others show no change. Herein we offer a reasonable explanation for this apparent controversy and present a range of antioxidant supplements that may offer some protection against testicular hyperthermia.
Detection of High-Grade CIN by Electrical Impedance Spectroscopy in Women Diagnosed with Low-Grade CIN on Cytology
Authors: Panagakis Georgios , Papapanagiotou Ioannis K , Theofanakis Charalampos , Tsetsa Paraskevi , Kontogeorgi Adamantia , Thomakos Nikolaos , Rodolakis Alexandros , Haidopoulos Dimitrios
Published: September 2023

The authors attempt to addresses the importance of timely detection and management of cervical intraepithelial neoplasia (CIN) to prevent cervical cancer. The study focuses on the potential of electrical impedance spectroscopy (EIS) as an adjunct to colposcopy, aiming to enhance the accuracy of identifying high-grade cervical lesions. Colposcopy, a widely used technique, exhibits variable sensitivity in detecting high-grade lesions, which relies on the expertise of the operator. The study's primary objective is to evaluate the effectiveness of combining colposcopy with EIS in detecting high-grade cervical lesions among patients initially diagnosed with low-grade CIN based on cytology. We employ a cross-sectional observational design, recruiting 101 women with abnormal cervical cytology results. The participants undergo colposcopy with acetic acid and subsequent EIS using the ZedScan device. The ZedScan results are categorized into color-coded probability levels, with red indicating the highest likelihood of high-grade squamous intraepithelial lesions (HSIL) occurrence. Results reveal that ZedScan exhibits a sensitivity rate of 89.5% and a specificity rate of 84% for detecting high-grade lesions. Colposcopy, on the other hand, records a sensitivity rate of 85.5% and a specificity rate of 92%. The agreement rate between ZedScan and biopsy is 79.2%, as indicated by a kappa coefficient of 0.71, while the agreement rate between colposcopy and biopsy is 74.3%, with a kappa coefficient of 0.71.
Effect of Nitrogen Levels on Methanogen and Methanotroph Populations in Rice Paddies
Authors: Kwon Youngho , Lee So-Myeong , Kang Ju-Won , Cha Jin-Kyung , Park Hyeonjin , Park Dong-Soo , Kabange Nkulu Rolly , Oh Ki-Won , Kwak Youn-Sig , Lee Jong-Hee
Published: September 2023

Rice production is a significant contributor to methane emissions, accounting for ap-proximately 11% of global anthropogenic emissions. However, methane emissions in rice fields could effectively be reduced by implementing proper management practices and careful cultivar selection. The impact of nitrogen fertilizers on methane emissions is mul-tifaceted, as these fertilizers enhance crop growth and influence the activity of me-thane-producing (methanogens) and methane-consuming microbes (methanotrophs), leading to complex outcomes in methane emissions. In this study, we used qPCR to quan-tify methanogens and methanotrophs using mcrA (methanogenesis-related gene) and pmoA (methane oxidation-related gene) primer sets under different nitrogen levels (0, 50, and 100%) and rice varieties. The results revealed that higher nitrogen input led to higher methanogen inhabitation in the rhizosphere. Additionally, the abundances of methano-gens and methanotrophs varied among the different rice varieties. Furthermore, it was observed that there may be an additive effect between the rice variety and nitrogen level used. These findings suggest that future breeding efforts should involve screening for me-thane-related microbes in rice cultivars adapted to low-nitrogen conditions. By identifying and selecting rice varieties that promote lower methanogen levels and higher metha-notroph inhabitation, significant steps can be taken to mitigate greenhouse gas emissions from rice cultivation.
Establishing Consensus on Essential Resources for Musculoskeletal Trauma Care Worldwide
Authors: MacKechnie Madeline C. , Shearer David W. , Verhofstad Michael H.J. , Martin Claude , Graham Simon M. , Pesantez Rodrigo , Schuetz Michael , Hüttl Tobias , Kojima Kodi , Bernstein Brian P. , Miclau Theodore ,   the Delphi Study Group
Published: September 2023

Background:

Despite evidence that formalized trauma systems enhance patient functional outcomes and decrease mortality rates, there remains a lack of such systems globally. Critical to trauma systems are the equipment, materials, and supplies needed to support care, which vary in availability regionally. The purpose of the present study was to identify essential resources for musculoskeletal trauma care across diverse resource settings worldwide.

Methods:

The modified Delphi method was utilized, with 3 rounds of electronic surveys. Respondents consisted of 1 surgeon with expertise in musculoskeletal trauma per country. Participants were identified with use of the AO Trauma, AO Alliance, Orthopaedic Trauma Association, and European Society for Trauma and Emergency Surgery networks. Respondents rated resources on a Likert scale from 1 (most important) to 9 (least important). The “most essential” resources were classified as those rated ≤2 by ≥75% of the sampled group.

Results:

One hundred and three of 111 invited surgeons completed the first survey and were included throughout the subsequent rounds (representing a 93% response rate). Most participants were fellowship-trained (78%) trauma and orthopaedic surgeons (90%) practicing in an academic setting (62%), and 46% had >20 years of experience. Respondents represented low-income and lower-middle-income countries (LMICs; 35%), upper-middle income countries (UMICs; 30%), and high-income countries (HICs; 35%). The initial survey identified 308 unique resources for pre-hospital, in-hospital, and post-hospital phases of care, of which 71 resources achieved consensus as the most essential. There was a significant difference (p < 0.0167) in ratings between income groups for 16 resources, all of which were related to general trauma care rather than musculoskeletal injury management.

Conclusions:

There was agreement on a core list of essential musculoskeletal trauma care resources by respondents from LMICs, UMICs, and HICs. All significant differences in resource ratings were related to general trauma management. This study represents a first step toward establishing international consensus and underscores the need to prioritize resources that are locally available. The information can be used to develop effective guidelines and policies, create best-practice treatment standards, and advocate for necessary resources worldwide.

Clinical Relevance:

This study utilized the Delphi method representing expert opinion; however, this work did not examine patient management and therefore does not have a clinical Level of Evidence.
Physician Assistant Use of Ultrasound for Shoulder, Hip, and Knee Joint Injections
Authors: Joseph Jennifer , Salisbury Helen
Published: September 2023

Background:

Physician assistants (PAs) perform joint injections in the office setting. Although ultrasound (US) provides a safe option that increases accuracy of injections, this orthopedic specific training has not been routinely offered in PA education. The aim of this study was to examine the frequency with which orthopedic PAs performed shoulder, hip, and knee joint injections, with and without US, and how US training was obtained.

Methods:

In a quantitative descriptive cross-sectional study, PAs received a link to a self-designed survey by an email list provided by the physician assistants in orthopedic surgery.

Results:

Most PAs with access to US performed 10 or less shoulder, hip, and knee joint injections using US each month. The most common US training reported was on-the-job (n = 141; 35.30%), independent courses (n = 91; 22.80%), and self-taught (n = 70; 17.50%). Nearly one-half (n = 196; 49.00%) reported no US training, leading to 177 PAs (60.00%) perceiving they were not at all or only somewhat prepared to perform US in clinical practice.

Conclusion:

Overall, shoulder and knee joint injections were performed more often in the office than hip joint injections and were more commonly performed without US. Most PAs perceived they were not prepared to perform US in practice during their formal education, with many having sought further training.
Infusing Intercultural Awareness in English Materials Development for 4th Grade at Islamic Elementary School
Authors: Ivana Nabilah Qoriroh Mujahidah  , Mahardika Fajar Tri , Yutisanto Iman , Roykhanah  , Riyan Ahmad , Sabilah Fardini
Published: September 2023

Embedding intercultural awareness in teaching English is an essential aspect that must be considered. This paper aims to develop the English materials for 4th grade at Islamic Elementary School. The researchers employed mixed-method research to do a need analysis and design the English materials. Choosing and Analyzing the English material from the 4th grade's book was the first step in collecting the data. Then, the researcher spread the questionnaires to the 13 teachers and 71 students as the research participants that have selected purposively. The data was analyzed using explanatory sequential mixed methods, in which the qualitative data was used to explain the quantitative data results. This paper revealed that teachers and students agreed that infusing intercultural awareness is essential to learning English. They also proposed the appropriate materials to be developed with the researchers, such as adding typical food and traditional festival in the introduction materials. After designing and developing the materials, the researcher finally produced online materials in the form of video. The researchers tried to implement the video to get constructive feedback. The students' and teachers' perspectives are almost the same. The video is fascinating, and the language level is medium, but the researchers must pay attention to the duration and video quality.
Exploring the intersections of college student poverty, grief, and racial/ethnic identity: a scoping review protocol
Authors: Garza-Levitt Sarah Elizabeth , McFarland Mary M. , Gonzalez Kimberly Ponce , Supiano Katherine P.
Published: September 2023

Background
College students who experience the negative impact of poverty, such as food, financial, and housing insecurity, are at higher risk for poor academic performance. And one recent study examined grief in a college student sample and found those students with a diverse racial or ethnic background were more likely to experience Prolonged Grief Disorder; however, did not examine potential poverty in their sample. To date, there do not appear to be studies that have examined poverty by racial and ethnic identity and the experience of grief due to the death of a family member or friend. Our aim is to map the literature reporting on college students of any age who identify or are identified as non-white racial or ethnic groups who experience poverty and grief due to the death of a family member or friend. Our second objective is to map the interventional supports in practice delivered from campus or community services to support student academic performance and degree completion.
Methods
We will conduct our scoping review with guidance from the latest version of the JBI Manual for Evidence Synthesis. Utilizing the framework as outlined by Arksey and O’Malley, we will conduct our scoping review with Arksey’s five stages: 1) identifying the research question, 2) identifying relevant studies, 3) study selection, 4) charting the data, and 5) collating, summarizing and reporting the results. For transparency and reproducibility, we will adhere to the PRISMA reporting guidelines.
Discussion
The purpose of this scoping review is to map the literature on the intersections of college student poverty, grief, and racial/ethnic identity. A limitation of this review is that all relevant studies may not be identified due to database selection (i.e., searching more databases may identify additional relevant studies). This review will contribute to the literature base on college student poverty and create greater awareness around the needs of racially and ethnically diverse students experiencing poverty and grief due to death. The results may be of value, particularly to college administrators developing prevention and interventional programs to support college student success.
Systematic review registration
Open Science Framework (https://osf.io/enuwt).