bluuebunny/crossref_metadata_2025 huggingface dataset:
Urinary Mass Spectrometry Profiles in Age-Related Macular Degeneration
Authors: Lains Ines , Mendez Kevin M. , Gil João Q. , Miller John B. , Kelly Rachel S. , Barreto Patrícia , Kim Ivana K. , Vavvas Demetrios G. , Murta Joaquim Neto , Liang Liming , Silva Rufino , Miller Joan W. , Lasky-Su Jessica , Husain Deeba
Published: February 2022

We and others have shown that patients with different severity stages of age-related macular degeneration (AMD) have distinct plasma metabolomic profiles compared to controls. Urine is a biofluid that can be obtained non-invasively and, in other fields, urine metabolomics has been proposed as a feasible alternative to plasma biomarkers. However, no studies have applied urinary mass spectrometry (MS) metabolomics to AMD. This study aimed to assess urinary metabolomic profiles of patients with different stages of AMD and a control group. We included two prospectively designed, multicenter, cross-sectional study cohorts: Boston, US (n = 185) and Coimbra, Portugal (n = 299). We collected fasting urine samples, which were used for metabolomic profiling (Ultrahigh Performance Liquid chromatography—Mass Spectrometry). Multivariable logistic and ordinal logistic regression models were used for analysis, accounting for gender, age, body mass index and use of AREDS supplementation. Results from both cohorts were then meta-analyzed. No significant differences in urine metabolites were seen when comparing patients with AMD and controls. When disease severity was considered as an outcome, six urinary metabolites differed significantly (p < 0.01). In particular, two of the metabolites identified have been previously shown by our group to also differ in the plasma of patients of AMD compared to controls and across severity stages. While there are fewer urinary metabolites associated with AMD than plasma metabolites, this study identified some differences across stages of disease that support previous work performed with plasma, thus highlighting the potential of these metabolites as future biomarkers for AMD.
COVID-19, Vaccines, and Thrombotic Events: A Narrative Review
Authors: Abrignani Maurizio G. , Murrone Adriano , De Luca Leonardo , Roncon Loris , Di Lenarda Andrea , Valente Serafina , Caldarola Pasquale , Riccio Carmine , Oliva Fabrizio , Gulizia Michele M. , Gabrielli Domenico , Colivicchi Furio ,   on behalf of the Working Group on Anti-COVID-19 Vaccination of the Associazione Nazionale Medici Cardiologi Ospedalieri (ANMCO)
Published: February 2022

The coronavirus disease 2019 (COVID-19), a deadly pandemic that has affected millions of people worldwide, is associated with cardiovascular complications, including venous and arterial thromboembolic events. Viral spike proteins, in fact, may promote the release of prothrombotic and inflammatory mediators. Vaccines, coding for the spike protein, are the primary means for preventing COVID-19. However, some unexpected thrombotic events at unusual sites, most frequently located in the cerebral venous sinus but also splanchnic, with associated thrombocytopenia, have emerged in subjects who received adenovirus-based vaccines, especially in fertile women. This clinical entity was soon recognized as a new syndrome, named vaccine-induced immune thrombotic thrombocytopenia, probably caused by cross-reacting anti-platelet factor-4 antibodies activating platelets. For this reason, the regulatory agencies of various countries restricted the use of adenovirus-based vaccines to some age groups. The prevailing opinion of most experts, however, is that the risk of developing COVID-19, including thrombotic complications, clearly outweighs this potential risk. This point-of-view aims at providing a narrative review of epidemiological issues, clinical data, and pathogenetic hypotheses of thrombosis linked to both COVID-19 and its vaccines, helping medical practitioners to offer up-to-date and evidence-based counseling to their often-alarmed patients with acute or chronic cardiovascular thrombotic events.
Associations between RNA-Binding Motif Protein 3, Fibroblast Growth Factor 21, and Clinical Outcome in Patients with Stroke
Authors: Ávila-Gómez Paulo , Pérez-Mato María , Hervella Pablo , Dopico-López Antonio , Silva-Candal Andrés da , Bugallo-Casal Ana , López-Amoedo Sonia , Candamo-Lourido María , Sobrino Tomás , Iglesias-Rey Ramón , Castillo José , Campos Francisco
Published: February 2022

Background: RNA-binding motif protein 3 (RBM3) is a cold-induced marker of good functional outcome of ischemic stroke that is promising as a protective target. Fibroblast growth factor 21 (FGF21) is an obesity- and temperature-related hormone that upregulates the expression of RBM3, which is beneficial as a recombinant treatment and has been tested under different experimental pathological conditions, including stroke. However, the interaction between RBM3 and FGF21 has not yet been tested for clinical stroke conditions. Methods: In a sample of 66 stroke patients, we analyzed the associations between the FGF21 and RBM3 serum concentrations on admission and at 72 h, body weight, maximum temperature during the first 24 h, and the outcome of patients at 3 months. We also analyzed their association with biomarkers of obesity (adiponectin and leptin) and inflammation (interleukin-6 (IL-6) and interleukin (IL-10)). Results: Higher concentrations of FGF21 on admission and RBM3 at 72 h were associated with good outcomes. Serum FGF21 and RBM3 were directly related to body mass index and inversely related to the maximum temperature during the first 24 h. We found a positive association between the FGF21 concentrations in obese patients with leptin and a negative correlation with adiponectin in non-obese participants. Conclusions: This clinical study demonstrates the association between RBM3 and FGF21 levels and the outcome of stroke patients. Although further investigations are required, these data support the pharmacological induction of RBM3 as a promising protective therapy.
Estimation of Gross Motor Functions in Children with Cerebral Palsy Using Zebris FDM-T Treadmill
Authors: Bedla Mariusz , Pięta Paweł , Kaczmarski Daniel , Deniziak Stanisław
Published: February 2022

A standardized observational instrument designed to measure change in gross motor function over time in children with cerebral palsy is the Gross Motor Function Measure (GMFM). The process of evaluating a value for the GMFM index can be time consuming. It typically takes 45 to 60 min for the patient to complete all tasks, sometimes in two or more sessions. The diagnostic procedure requires trained and specialized therapists. The paper presents the estimation of the GMFM measure for patients with cerebral palsy based on the results of the Zebris FDM-T treadmill. For this purpose, the regression analysis was used. Estimations based on the Generalized Linear Regression were assessed using different error metrics. The results obtained showed that the GMFM score can be estimated with acceptable accuracy. Because the Zebris FDM-T is a widely used device in gait rehabilitation, our method has the potential to be widely adopted for objective diagnostics of children with cerebral palsy.
Rapidity and Precision of Steroid Hormone Measurement
Authors: Karashima Shigehiro , Osaka Issey
Published: February 2022

Steroids are present in all animals and plants, from mammals to prokaryotes. In the medical field, steroids are commonly classified as glucocorticoids, mineralocorticoids, and gonadal steroid hormones. Monitoring of hormones is useful in clinical and research fields for the assessment of physiological changes associated with aging, disease risk, and the diagnostic and therapeutic effects of various diseases. Since the discovery and isolation of steroid hormones, measurement methods for steroid hormones in biological samples have advanced substantially. Although immunoassays (IAs) are widely used in daily practice, mass spectrometry (MS)-based methods have been reported to be more specific. Steroid hormone measurement based on MS is desirable in clinical practice; however, there are several drawbacks, including the purchase and maintenance costs of the MS instrument and the need for specialized training of technicians. In this review, we discuss IA- and MS-based methods currently in use and briefly present the history of steroid hormone measurement. In addition, we describe recent advances in IA- and MS-based methods and future applications and considerations.
Low Serum Levels of Interferon Alpha in COVID-19 Patients Are Associated with Older Age
Authors: Alidjinou Enagnon Kazali , Hirabidian Mickael , Rabat Anthony , Ouafi Mahdi , Nekoua Magloire Pandoua , Sane Famara , Poissy Julien , Hober Didier ,   The Lille COVID Research Network (LICORNE)
Published: February 2022

Innate immune response, especially type 1 interferon (IFN) response is considered to play a substantial role in the outcome of SARS-CoV-2 infection. A reduced and delayed IFN response has been associated with progression to severe COVID-19. In this study, we investigated levels of circulating IFNα and serum neutralizing activity in COVID-19 patients admitted to the intensive care unit. We found a significant association of levels of IFNα with age (p = 0.007). This association has also been observed in a cohort of COVID-19 outpatients with mild infection (p = 0.02). The impact of senescence on IFN response can explain the higher susceptibility of the elderly to severe COVID-19.
Non-Alcoholic Fatty Liver Disease and Risk of Macro- and Microvascular Complications in Patients with Type 2 Diabetes
Authors: Mantovani Alessandro , Dalbeni Andrea , Beatrice Giorgia , Cappelli Davide , Gomez-Peralta Fernando
Published: February 2022

Non-alcoholic fatty liver disease (NAFLD) is considered the hepatic manifestation of metabolic syndrome. To date, NAFLD is the most frequent chronic liver disease seen day by day in clinical practice across most high-income countries, affecting nearly 25–30% of adults in the general population and up to 70% of patients with T2DM. Over the last few decades, it clearly emerged that NAFLD is a “multisystemic disease” and that the leading cause of death among patients with NAFLD is cardiovascular disease (CVD). Indeed, several observational studies and some meta-analyses have documented that NAFLD, especially its advanced forms, is strongly associated with fatal and non-fatal cardiovascular events, as well as with specific cardiac complications, including sub-clinical myocardial alteration and dysfunction, heart valve diseases and cardiac arrhythmias. Importantly, across various studies, these associations remained significant after adjustment for established cardiovascular risk factors and other confounders. Additionally, several observational studies and some meta-analyses have also reported that NAFLD is independently associated with specific microvascular conditions, such as chronic kidney disease and distal or autonomic neuropathy. Conversely, data regarding a potential association between NAFLD and retinopathy are scarce and often conflicting. This narrative review will describe the current evidence about the association between NAFLD and the risk of macro- and microvascular manifestations of CVD, especially in patients with T2DM. We will also briefly discuss the biological mechanisms underpinning the association between NAFLD and its advanced forms and macro- and microvascular CVD.
Epidemiological Survey of Different Treatments for Choledocholithiasis in Taiwan: A Nationwide, Population-Based Cohort Analysis
Authors: Chen Jia-Hui , Chung Chi-Hsiang , Li Chung-Hsien , Chien Wu-Chien , Chang Chao-Feng
Published: February 2022

(1) Background: Open, laparoscopic, and endoscopic choledocholithotomy (OC, LC, and EC, respectively) are accepted choledocholithiasis treatment modalities. However, an assessment of the nationwide trends in their outcomes is lacking. This nationwide population-based analysis evaluated treatment outcomes of choledocholithiasis in Taiwan; (2) Methods: A total of 13,139,306 individuals were randomly enrolled from the Longitudinal Health Insurance Database (LHID) between 2000 to 2013 for cohort analysis. All patients with newly diagnosed choledocholithiasis aged 18 years or older who were treated during the study period were enrolled and allocated to the OC, LC, EC, or combined endoscopy and open choledocholithotomy (CEOC) groups. Age, readmission, retained stone, comorbidities, hospital stay, medical cost, complications, mortality were analyzed; (3) Results: A total of 58,064 individuals met the inclusion criteria, including 46.54%, 1.10%, 47.52%, and 4.85% who underwent OC, LC, EC, and CEOC, respectively. The endpoint characteristics showed that the LC group had higher readmission, longer hospital stay, and higher medical cost. Cox regression analysis showed that the adjusted hazard ratio (HR) of complications for EC was 1.259 times higher than that for OC. The adjusted HRs of readmission within 90 days for LC, EC, and CEOC were higher than that of OC. The adjusted HR of retreatment with surgery was higher in LC. The adjusted HR of retreatment with endoscopy was higher in CEOC. The adjusted HR of mortality in EC was 1.603 times that of OC; (4) Conclusions: Different choledocholithiasis treatments lead to different outcomes. However, further studies on other large or national data sets are required to support these findings.
Self-Reported Impaired Wound Healing in Young Adults and Their Susceptibility to Experiencing Immune-Related Complaints
Authors: Balikji Jessica , Hoogbergen Maarten M. , Garssen Johan , Verster Joris C.
Published: February 2022

The current study examined to what extent individuals with wound infection (WI group), slow healing wounds (SHW group), or both (COMBI group) report poorer immune fitness and whether they experience immune-related complaints more often as compared to healthy participants (control group). Survey data from 3613 Dutch students was re-analyzed. Compared to the control group, perceived immune fitness was significantly lower by the SHW group (p < 0.001) and the COMBI group (p < 0.001), but no difference was found for the WI group (p = 0.059). Also, perceived immune fitness of the COMBI group was significantly worse compared to the WI group (p = 0.040). Compared to the control group, reduced immune fitness was reported to be significantly more frequently by the SHW group (p < 0.001) and the COMBI group (p < 0.001). Reduced immune fitness was significantly more common for the COMBI group compared to the SHW group (p = 0.011) and WI group (p = 0.001). Immune-related complaints such as headache, runny nose, coughing, sore throat, diarrhea, flu, and fever were significantly more frequently reported by individuals with impaired wound healing. The effects were most pronounced in the COMBI group, followed by the SHW group and a lesser extent the WI group. A highly significant correlation was found between perceived immune fitness and the percentage of individuals that reported impaired wound healing. In conclusion, the findings confirm that poorer immune functioning is characteristic for individuals with impaired wound healing. In follow-up studies, immune biomarkers analyses are needed to support patient-reported outcome measures.
COVID-19-Associated Encephalopathy—Case Series and Clinical Considerations
Authors: Udzik Jakub , Jakubowski Paweł , Niekrasz Małgorzata , Barczyszyn Adrian , Parczewski Miłosz
Published: February 2022

Neurological manifestations of the SARS-CoV-2 infection are present in up to 80% of the affected patients. While the majority of them is benign, in certain patients, viral replication in the central nervous system results in a severe disruption in cognitive function as well as basic life functions. In this case series, the authors present a detailed description of the three SARS-CoV-2 infection cases, which were all complicated by severe encephalopathy. Consecutive neurological status changes were described for each patient with detailed imaging and clinical sequelae. In the discussion, the authors highlight similarities in the course of the disease in presented patients, as well as common features in test results. An effective causal treatment could not be introduced in any of the patients, nor could the progression of the central nervous system (CNS) damage be stopped. The authors hope that the experiences they gathered will help to accelerate the diagnostic and therapeutic process in other patients with COVID-19-associated encephalopathy and result in introducing an effective treatment.
Elevated Plasma Soluble C-Type Lectin-like Receptor 2 Is Associated with the Worsening of Coronavirus Disease 2019
Authors: Wada Hideo , Ichikawa Yuhuko , Ezaki Minoru , Yamamoto Akitaka , Tomida Masaki , Yoshida Masamichi , Fukui Shunsuke , Moritani Isao , Shiraki Katsuya , Shimaoka Motomu , Iba Toshiaki , Suzuki-Inoue Katsue , Shimpo Hideto
Published: February 2022

Although thrombosis in coronavirus disease 2019 (COVID-19) infection has attracted attention, the mechanism underlying its development remains unclear. The relationship between platelet activation and the severity of COVID-19 infection was compared with that involving other infections. Plasma soluble C-type lectin-like receptor 2 (sCLEC-2) levels were measured in 46 patients with COVID-19 infection and in 127 patients with other infections. The plasma sCLEC-2 levels in patients with COVID-19 infection {median (25th, 75th percentile), 489 (355, 668) ng/L} were significantly higher (p < 0.001) in comparison to patients suffering from other pneumonia {276 (183, 459) ng/L}, and the plasma sCLEC-2 levels of COVID-19 patients with severe {641 (406, 781) ng/L} or critical illness {776 (627, 860) ng/L} were significantly higher (p < 0.01, respectively) in comparison to those with mild illness {375 (278, 484) ng/L}. The ratio of the sCLEC-2 levels to platelets in COVID-19 patients with critical illness of infection was significantly higher (p < 0.01, p < 0.001 and p < 0.05, respectively) in comparison to COVID-19 patients with mild, moderate or severe illness. Plasma sCLEC-2 levels were significantly higher in patients with COVID-19 infection than in those with other infections, suggesting that platelet activation is triggered and facilitated by COVID-19 infection.
HEAD Access Control Metamodel: Distinct Design, Advanced Features, and New Opportunities
Authors: Kashmar Nadine , Adda Mehdi , Ibrahim Hussein
Published: February 2022

Access control (AC) policies are a set of rules administering decisions in systems and they are increasingly used for implementing flexible and adaptive systems to control access in today’s internet services, networks, security systems, and others. The emergence of the current generation of networking environments, with digital transformation, such as the internet of things (IoT), fog computing, cloud computing, etc., with their different applications, bring out new trends, concepts, and challenges to integrate more advanced and intelligent systems in critical and heterogeneous structures. This fact, in addition to the COVID-19 pandemic, has prompted a greater need than ever for AC due to widespread telework and the need to access resources and data related to critical domains such as government, healthcare, industry, and others, and any successful cyber or physical attack can disrupt operations or even decline critical services to society. Moreover, various declarations have announced that the world of AC is changing fast, and the pandemic made AC feel more essential than in the past. To minimize security risks of any unauthorized access to physical and logical systems, before and during the pandemic, several AC approaches are proposed to find a common specification for security policy where AC is implemented in various dynamic and heterogeneous computing environments. Unfortunately, the proposed AC models and metamodels have limited features and are insufficient to meet the current access control requirements. In this context, we have developed a Hierarchical, Extensible, Advanced, and Dynamic (HEAD) AC metamodel with substantial features that is able to encompass the heterogeneity of AC models, overcome the existing limitations of the proposed AC metamodels, and follow the various technology progressions. In this paper, we explain the distinct design of the HEAD metamodel, starting from the metamodel development phase and reaching to the policy enforcement phase. We describe the remaining steps and how they can be employed to develop more advanced features in order to open new opportunities and answer the various challenges of technology progressions and the impact of the pandemic in the domain. As a result, we present a novel approach in five main phases: metamodel development, deriving models, generating policies, policy analysis and assessment, and policy enforcement. This approach can be employed to assist security experts and system administrators to design secure systems that comply with the organizational security policies that are related to access control.
Facile Fabrication of Magnetic Poly(Vinyl Alcohol)/Activated Carbon Composite Gel for Adsorptive Removal of Dyes
Authors: Wen Tao , Huang Baotao , Zhou Li
Published: February 2022

Activated carbon (AC) has been widely utilized for the adsorption of pollutants from water. However, it is difficult to recycle the AC after adsorption. In this paper, we report a facile one-pot approach to fabricate magnetic poly(vinyl alcohol)/AC composite gel (mPVA/AC CG) by dropwise addition of an aqueous mixture of PVA, AC and iron ions into the ammonia solution. The obtained mPVA/AC CG after freeze-drying shows porous microstructure and favorable magnetic properties. The utilization of mPVA/AC CG for adsorptive removal of methylene blue (MB) and methyl orange (MO) dyes from water was investigated. The mPVA/AC CG not only exhibited good adsorption performance for both MB and MO dyes but also could be readily recycled using a magnet after adsorption. The adsorption process was well described by the pseudo-second-order kinetic model and the Langmuir isotherm model. Considering the simple fabrication process, good adsorption performance and favorable magnetic separation capability, this work provides a viable strategy for combining the features of AC and magnetic gel for fabrication of applicable magnetic adsorbent.
Training Load, Maturity Timing and Future National Team Selection in National Youth Basketball Players
Authors: Arede Jorge , Freitas Tomás T. , Johnson David , Fernandes John F. T. , Williams Sean , Moran Jason , Leite Nuno
Published: February 2022

Despite its importance to the management of training stress, monotony and recovery from exercise, training load has not been quantified during periods of intensity training in youths. This study aimed to (1) examine and quantify the training load (TL) in youth national team basketball players during a 2-week training camp according to maturity timing and (2) determine which parameters were related to under-18 (U18) national team selection. Twenty-nine U-16 national team basketball players underwent an anthropometric assessment to determine maturity timing. Players were categorised by maturity timing (early vs. average), whilst TL parameters during a 2-week training camp (i.e., 21 sessions) prior to FIBA U16 European Championship were used for group comparison and to predict future U-18 national team selection. The early-maturing players, who were taller and heavier (p < 0.05), experienced greater training strain in week 1 (p < 0.05) only. Irrespective of maturity timing, training loads in week 2 were predictive of onward selection for the U-18 national team. Conclusion: Based on present findings, practitioners are encouraged to develop their athletes’ ability to tolerate high weekly loads, but also to be mindful that athletes’ perceived exertion during national team training may be influenced by maturity timing.
Heat Shock Transcription Factor CgHSF1 Is Required for Melanin Biosynthesis, Appressorium Formation, and Pathogenicity in Colletotrichum gloeosporioides
Authors: Gao Xuesheng , Wang Qiannan , Feng Qingdeng , Zhang Bei , He Chaozu , Luo Hongli , An Bang
Published: February 2022

Heat shock transcription factors (HSFs) are a family of transcription regulators. Although HSFs’ functions in controlling the transcription of the molecular chaperone heat shock proteins and resistance to stresses are well established, their effects on the pathogenicity of plant pathogenic fungi remain unknown. In this study, we analyze the role of CgHSF1 in the pathogenicity of Colletotrichum gloeosporioides and investigate the underlying mechanism. Failure to generate the Cghsf1 knock-out mutant suggested that the gene is essential for the viability of the fungus. Then, genetic depletion of the Cghsf1 was achieved by inserting the repressive promoter of nitrite reductase gene (PniiA) before its coding sequence. The mutant showed significantly decrease in the pathogenicity repression of appressorium formation, and severe defects in melanin biosynthesis. Moreover, four melanin synthetic genes were identified as direct targets of CgHSF1. Taken together, this work highlights the role of CgHSF1 in fungal pathogenicity via the transcriptional activation of melanin biosynthesis. Our study extends the understanding of fungal HSF1 proteins, especially their involvement in pathogenicity.
SWAAT Bioinformatics Workflow for Protein Structure-Based Annotation of ADME Gene Variants
Authors: Othman Houcemeddine , Jemimah Sherlyn , da Rocha Jorge Emanuel Batista
Published: February 2022

Recent genomic studies have revealed the critical impact of genetic diversity within small population groups in determining the way individuals respond to drugs. One of the biggest challenges is to accurately predict the effect of single nucleotide variants and to get the relevant information that allows for a better functional interpretation of genetic data. Different conformational scenarios upon the changing in amino acid sequences of pharmacologically important proteins might impact their stability and plasticity, which in turn might alter the interaction with the drug. Current sequence-based annotation methods have limited power to access this type of information. Motivated by these calls, we have developed the Structural Workflow for Annotating ADME Targets (SWAAT) that allows for the prediction of the variant effect based on structural properties. SWAAT annotates a panel of 36 ADME genes including 22 out of the 23 clinically important members identified by the PharmVar consortium. The workflow consists of a set of Python codes of which the execution is managed within Nextflow to annotate coding variants based on 37 criteria. SWAAT also includes an auxiliary workflow allowing a versatile use for genes other than ADME members. Our tool also includes a machine learning random forest binary classifier that showed an accuracy of 73%. Moreover, SWAAT outperformed six commonly used sequence-based variant prediction tools (PROVEAN, SIFT, PolyPhen-2, CADD, MetaSVM, and FATHMM) in terms of sensitivity and has comparable specificity. SWAAT is available as an open-source tool.
Evidence of Better Autonomic, Metabolic and Psychological Profile in Breast Cancer Survivors Meeting Current Physical Activity Recommendations: An Observational Study
Authors: Lucini Daniela , Malacarne Mara , Gatzemeier Wolfgang , Pagani Eleonora , Bernardelli Giuseppina , Parati Gianfranco , Pagani Massimo
Published: February 2022

The increased cardiometabolic risk observed in breast cancer survivors (BCS) is due to multiple mechanisms: Hormonal and immunological dysfunction are well-identified ones, while cardiac autonomic regulation (CAR) is less recognized but may play a new complementary role particularly relevant when considering conditions and behaviors associated with a better prognosis in BCS, such as physical training. This observational study investigated a group of consecutive (172) BCS subdivided in two groups: those who reached the physical activity goals above 600 (MET·min/week) and those who did not. We assessed CAR by autoregressive spectral analysis of cardiovascular variabilities (considering in particular the unitary autonomic nervous system index—ANSI), body mass composition, stress perception and lifestyle in order to verify possible differences due to execution of physical activity. Subjects who spontaneously met physical activity recommendations presented a better autonomic, metabolic and psychological profile compared to those who did not. Lower physical activity volume, poor metabolic parameters, increased stress and fatigue perception may cluster together, leading to worsened CAR. This control mechanism may play a complementary role in determining the increased cardiometabolic risk observed in BCS. Furthermore, it may also explain, albeit in part, the better prognosis observed in patients following interventions aiming to improve the sympathetic–parasympathetic balance, such as physical training, using a personalized medicine approach.
Conjoint Analysis: A Research Method to Study Patients’ Preferences and Personalize Care
Authors: Al-Omari Basem , Farhat Joviana , Ershaid Mai
Published: February 2022

This article aims to describe the conjoint analysis (CA) method and its application in healthcare settings, and to provide researchers with a brief guide to conduct a conjoint study. CA is a method for eliciting patients’ preferences that offers choices similar to those in the real world and allows researchers to quantify these preferences. To identify literature related to conjoint analysis, a comprehensive search of PubMed (MEDLINE), EMBASE, Web of Science, and Google Scholar was conducted without language or date restrictions. To identify the trend of publications and citations in conjoint analysis, an online search of all databases indexed in the Web of Science Core Collection was conducted on the 8th of December 2021 without time restriction. Searching key terms covered a wide range of synonyms related to conjoint analysis. The search field was limited to the title, and no language or date limitations were applied. The number of published documents related to CA was nearly 900 during the year 2021 and the total number of citations for CA documents was approximately 20,000 citations, which certainly shows that the popularity of CA is increasing, especially in the healthcare sciences services discipline, which is in the top five fields publishing CA documents. However, there are some limitations regarding the appropriate sample size, quality assessment tool, and external validity of CA.
Combined Motor and Cognitive Rehabilitation: The Impact on Motor Performance in Patients with Mild Cognitive Impairment. Systematic Review and Meta-Analysis
Authors: Kiper Pawel , Richard Michelle , Stefanutti Françoise , Pierson-Poinsignon Romain , Cacciante Luisa , Perin Cecilia , Mazzucchelli Miryam , Viganò Barbara , Meroni Roberto
Published: February 2022

Mild cognitive impairment (MCI), a neurodegenerative disease leading to Alzheimer’s disease or dementia, is often associated with physical complaints. Combined physical and cognitive training (PCT) has been investigated to see the effects on cognitive function, but its impact on motor functions and activities of daily living has not been explored yet. The combination of physical and cognitive training may be a valuable non-pharmacological intervention that could preserve motor function and quality of life (QoL). We aimed, therefore, to analyze if combined PCT is effective at improving motor performance in patients with an MCI. A systematic electronic literature search and a meta-analysis were conducted. The following criteria were compulsory for inclusion in the study: (1) randomized controlled trial design; (2) combined PCT compared to motor training alone or no intervention; (3) motor outcomes as a study’s end point. Nine articles met the inclusion criteria. Results showed that PCT significantly enhances balance compared to motor training alone (SMD 0.56; 95% CI 0.07 to 1.06; I2 = 59%; 160 participants), whereas a significant improvement was found for mobility in the PCT group when compared to no intervention (MD −1.80; 95% CI −2.70 to −0.90; I2 = 0%; 81 participants). However, there is no evidence that people with MCI experience an increase in gait speed and QoL at the end of their practice sessions. Further investigation with larger samples and a longer period of monitoring after intervention should be undertaken.
Language Assessment Literacy of Middle School English Teachers in Mexico
Authors: Bustamante Analynn
Published: February 2022

Because English is an integral component of education in Mexico, it is necessary to explore teachers’ language assessment literacy (LAL), or their language assessment knowledge and practices. Previous LAL studies have been performed in standardized testing-focused. However, the present study aims to explore the LAL of teachers of middle school English students in a context where governmental policies strive to engender communicative language learning, specifically, Mexico. I have taken a mixed methods approach which involved a survey (N = 123) and interviews at two locations in Mexico. The semi-structured interviews were conducted at one bilingual middle school (N = 7) and in one extracurricular English program (N = 6). Participants were asked about their previous training, confidence levels in their assessment practices, and their training needs. Findings suggest a divide among teachers with higher and lower levels of LAL. Participants indicated that their training primarily covered traditional forms of assessment and classroom-level decision-making. However, data also suggest that participants valued non-traditional assessment activities. Finally, participants desired training on topics such as the use of technology in assessment, unfamiliar non-traditional assessment activities, and program-level decision-making.
How Cognitive Abilities May Support Children’s Bilingual Literacy Development in a Multilingual Society
Authors: Vogelzang Margreet , Tsimpli Ianthi Maria , Panda Minati
Published: February 2022

Underprivileged but highly multilingual Indian children often show low literacy performance. As a complicating factor, these children are often expected to develop literacy not just in the regionally dominant language but also in English. As good literacy skills are crucial for later academic development, it is important to identify factors that could support these children’s literacy development. We, therefore, investigated whether cognitive abilities are associated with literacy development and whether they are so in the same way for both of these children’s languages. In a longitudinal design (Std. 4 and Std. 5), literacy data in Hindi and English were collected from 336 children in Delhi, India. In addition, three cognitive tasks (Raven’s, 2-back, Flanker) were performed. We found that bilingual literacy development is evident across children, although the starting point is low in some cases. Fluid intelligence (Raven’s) and working memory capacity (2-back) significantly positively related to literacy performance in Std. 4 and Std. 5 in both Hindi and English. Literacy improvement from Std. 4 to Std. 5 also related to cognitive abilities—working memory capacity (2-back) for Hindi and inhibitory skills (Flanker) for English—but in the opposite direction: Children who had lower scores on these cognitive tasks show more improvement, indicating that they are in the process of catching up with their higher-performing peers—although they have not fully managed to do so by Std. 5.
Regulation of Oxidative Stress by Long Non-Coding RNAs in Vascular Complications of Diabetes
Authors: Chu Pei-Ming , Yu Cheng-Chia , Tsai Kun-Ling , Hsieh Pei-Ling
Published: February 2022

Diabetes mellitus is a well-known metabolic disorder with numerous complications, such as macrovascular diseases (e.g., coronary heart disease, diabetic cardiomyopathy, stroke, and peripheral vascular disease), microvascular diseases (e.g., diabetic nephropathy, retinopathy, and diabetic cataract), and neuropathy. Multiple contributing factors are implicated in these complications, and the accumulation of oxidative stress is one of the critical ones. Several lines of evidence have suggested that oxidative stress may induce epigenetic modifications that eventually contribute to diabetic vascular complications. As one kind of epigenetic regulator involved in various disorders, non-coding RNAs have received great attention over the past few years. Non-coding RNAs can be roughly divided into short (such as microRNAs; ~21–25 nucleotides) or long non-coding RNAs (lncRNAs; >200 nucleotides). In this review, we briefly discussed the research regarding the roles of various lncRNAs, such as MALAT1, MEG3, GAS5, SNHG16, CASC2, HOTAIR, in the development of diabetic vascular complications in response to the stimulation of oxidative stress.
Casein Microgels as Benzydamine Hydrochloride Carriers for Prolonged Release
Authors: Milenkova Sofia , Pilicheva Bissera , Uzunova Yordanka , Yovcheva Temenuzhka , Marudova Maria
Published: February 2022

This research aims to investigate the properties of nano- and micro-sized casein hydrogels crosslinked by sodium tripolyphosphate as drug delivery systems. Benzydamine hydrochloride was chosen as a model hydrophilic drug. The gels were synthesized by varying different parameters: casein concentration, casein/crosslinking ratio, and addition of ethanol as a co-solvent. The electrostatic attractive interactions between the casein and the sodium tripolyphosphate were confirmed by FTIR spectroscopy. The particle sizes was determined by dynamic light scattering and varied in the range between several hundred nanometers and several microns. The yield of the gelation process was high for all investigated samples and varied between 55.3% and 78.3%. The encapsulation efficiency of the particles was strongly influenced by the casein concentration and casein/crosslinker ratio and its values were between 4.6% and 22.4%. The release study confirmed that casein particles are useful as benzydamine carriers and ensured prolonged release over 72 h.
Evaluation of Physical Properties of Zirconia Suspension with Added Silane Coupling Agent for Additive Manufacturing Processes
Authors: Jang Jae-Gon , Kang Jin-Ho , Joe Kwang-Bum , Sakthiabirami Kumaresan , Jang Kyoung-Jun , Jun Mee-Jin , Oh Gye-Jeong , Park Chan , Park Sang-Won
Published: February 2022

In this study, we have analysed the effects of a silane coupling agent on the volume fraction of zirconia for digital light processing (DLP)-based additive manufacturing processes. Zirconia suspension was prepared by the incorporation of silane-modified zirconia particles (experimental group) or untreated zirconia particles (control group). Furthermore, the control and experimental group were subdivided into three groups based on the volume fraction (52, 54, and 56 vol%) of zirconia particles. The disk-shaped zirconia samples were 3D (three-dimensional) printed using the DLP technique and their physical and mechanical properties were evaluated. The addition of a silane coupling agent to the zirconia samples was found to have influence of about 6% on the hardness and biaxial flexural strength. Moreover, the decrease in minute air gaps inside the zirconia layers significantly increased the material density (visualized from the microstructure analysis). Thus, from this study, it was established that the silane-modified zirconia particles had a positive effect on the physical properties of the zirconia parts.
Developing Fused Deposition Modeling Additive Manufacturing Processing Strategies for Aluminum Alloy 7075: Sample Preparation and Metallographic Characterization
Authors: Ding Huan , Zeng Congyuan , Raush Jonathan , Momeni Kasra , Guo Shengmin
Published: February 2022

Currently, no commercial aluminum 7000 series filaments are available for making aluminum parts using fused deposition modeling (FDM)-based additive manufacturing (AM). The key technical challenge associated with the FDM of aluminum alloy parts is consolidating the loosely packed alloy powders in the brown-body, separated by thin layers of surface oxides and polymer binders, into a dense structure. Classical pressing and sintering-based powder metallurgy (P/M) technologies are employed in this study to assist the development of FDM processing strategies for making strong Al7075 AM parts. Relevant FDM processing strategies, including green-body/brown-body formation and the sintering processes, are examined. The microstructures of the P/M-prepared, FDM-like Al7075 specimens are analyzed and compared with commercially available FDM 17-4 steel specimens. We explored the polymer removal and sintering strategies to minimize the pores of FDM-Al7075-sintered parts. Furthermore, the mechanisms that govern the sintering process are discussed.
Comparison of Testing Methods for Evaluating the Resistance of Alkali-Activated Blast Furnace Slag Systems to Sulfur Dioxide
Authors: Hrubý Petr , Kalina Lukáš , Bílek Vlastimil , Keprdova Sarka , Másilko Jiří , Plšková Iveta , Koplík Jan , Topolář Libor
Published: February 2022

Alkali-activated systems (AAS) represent an ecologically and economically sustainable inorganic binder as an alternative to ordinary Portland cement (OPC). One of the main benefits of AAS is their durability in aggressive environments, which can be equal or even better than that of OPC. In this paper, the influence of the type of alkaline activator in alkali-activated blast furnace slag (AAS) in terms of resistance to sulfur dioxide corrosion was investigated. The durability testing process was based on the CSN EN ISO 3231 standard and simultaneously compared with mortar samples prepared by using Blastfurnace cement CEM III/A 32.5R. The degradation progress was evaluated by employing several different methods such as observing the compressive strength development, weight change evaluation, non-destructive testing methods like ultrasound or impact echo technique, or visual phenolphthalein technique. Subsequently, fundamental characterization of samples by the XRD method was performed during the degradation test. The obtained results indicate that none of the testing methods used could be prioritized over others to determine the resistance of AAS against the action of sulfur dioxide. For this reason, the durability testing of AAS remains an issue, and the development of specific standards considering the behavior of AAS seems necessary.
Physico-Chemical Characteristics and Posterolateral Fusion Performance of Biphasic Calcium Phosphate with Submicron Needle-Shaped Surface Topography Combined with a Novel Polymer Binder
Authors: Belluomo Ruggero , Arriola-Alvarez Inazio , Kucko Nathan W. , Walsh William R. , de Bruijn Joost D. , Oliver Rema A. , Wills Dan , Crowley James , Wang Tian , Barrère-de Groot Florence
Published: February 2022

A biphasic calcium phosphate with submicron needle-shaped surface topography combined with a novel polyethylene glycol/polylactic acid triblock copolymer binder (BCP-EP) was investigated in this study. This study aims to evaluate the composition, degradation mechanism and bioactivity of BCP-EP in vitro, and its in vivo performance as an autograft bone graft (ABG) extender in a rabbit Posterolateral Fusion (PLF) model. The characterization of BCP-EP and its in vitro degradation products showed that the binder hydrolyses rapidly into lactic acid, lactide oligomers and unaltered PEG (polyethylene glycol) without altering the BCP granules and their characteristic submicron needle-shaped surface topography. The bioactivity of BCP-EP after immersion in SBF revealed a progressive surface mineralization. In vivo, BCP-EP was assessed in a rabbit PLF model by radiography, manual palpation, histology and histomorphometry up to 12 weeks post-implantation. Twenty skeletally mature New Zealand (NZ) White Rabbits underwent single-level intertransverse process PLF surgery at L4/5 using (1) autologous bone graft (ABG) alone or (2) by mixing in a 1:1 ratio with BCP-EP (BCP-EP/ABG). After 3 days of implantation, histology showed the BCP granules were in direct contact with tissues and cells. After 12 weeks, material resorption and mature bone formation were observed, which resulted in solid fusion between the two transverse processes, following all assessment methods. BCP-EP/ABG showed comparable fusion rates with ABG at 12 weeks, and no graft migration or adverse reaction were noted at the implantation site nor in distant organs.
Thermal Insulation Mattresses Based on Textile Waste and Recycled Plastic Waste Fibres, Integrating Natural Fibres of Vegetable or Animal Origin
Authors: Hegyi Andreea , Vermeșan Horațiu , Lăzărescu Adrian-Victor , Petcu Cristian , Bulacu Cezar
Published: February 2022

The current context provides, worldwide, the need to identify solutions for the thermal efficiency of constructions, through sustainable and innovative methods and products. A viable solution is to produce thermal insulating products by carding-folding technology, using natural fibres and recycled polyethylene terephthalate (rPET) and polyester (rPES) waste, converted to fibres. This paper presents experimental results obtained after testing several thermal insulation composite products produced using a mix of sheep wool, cellulose, rPET and rPES fibres. The results of the research demonstrate the thermal insulation properties but, at the same time, identify the benefits of using such materials on the quality of the air in the interior space (the ability to adjust humidity and reduce the concentration of harmful substances). At the same time, the advantages of using sheep wool composite mattresses concerning their resistance to insect attack is demonstrated when compared with ordinary thermal insulation materials. Finally, sensitivity elements of these composites are observed in terms of sensitivity to mould, and to contact with water or soil, drawing future research directions in the development of this type of materials.
Influence of the Mix Proportion and Aggregate Features on the Performance of Eco-Efficient Fine Recycled Concrete Aggregate Mixtures
Authors: De Souza Diego Jesus , de Grazia Mayra T. , Macedo Hian F. , Sanchez Leandro F. M. , de Andrade Gabriella P. , Naboka Olga , Fathifazl Gholamreza , Nkinamubanzi Pierre-Claver
Published: February 2022

Most of the previous research on recycled concrete aggregates (RCA) has focused on coarse RCA (CRCA), while much less has been accomplished on the use of fine RCA particles (FRCA). Furthermore, most RCA research disregards its unique microstructure, and thus the inferior performance of concrete incorporating RCA is often reported in the fresh and hardened states. To improve the overall behaviour of RCA concrete advanced mix design techniques such as equivalent volume (EV) or particle packing models (PPMs) may be used. However, the efficiency of these procedures to proportion eco-efficient FRCA concrete still requires further investigation. This work evaluates the overall fresh (i.e., slump and rheological characterization) and hardened states (i.e., non-destructive tests, compressive strength and microscopy) performance of sustainable FRCA mixtures proportioned through distinct techniques (i.e., direct replacement, EV and PPMs) and incorporating different types of aggregates (i.e., natural and manufactured sand) and manufacturing processes (i.e., crusher fines and fully ground). Results demonstrate that the aggregate type and crushing process may influence the FRCA particles’ features. Yet, the use of advanced mix design techniques, particularly PPMs, may provide FRCA mixes with quite suitable performance in the fresh (i.e., 49% lower yield stress) and hardened states (i.e., 53% higher compressive strength) along with a low carbon footprint.