bluuebunny/crossref_metadata_2025 huggingface dataset:
Diverse Understory Vegetation Alleviates Nitrogen Competition with Crop Trees in Poplar Plantations
Authors: Zhang Jiayu , Qin Guangzhen , Zhai Zheng , Zhou Shichao , Tang Luozhong , Tian Ye
Published: May 2021

Understory vegetation plays a crucial role in nutrient turnover and cycling in plantations, but it also competes for nutrients with crop trees when only a single species is present due to its specific nutrient requirements. However, it remains unclear whether this competition can be alleviated when the species richness of understory vegetation increases. In this study, we tested different gradients of understory vegetation species richness, including understory vegetation removal (UR), the retention of a single main understory vegetation species (RS), and the retention of natural diverse understory vegetation (RD) as part of a poplar (Populus deltoides ‘Nanlin-3804′) plantation, to study their effects on poplar growth, and to evaluate nitrogen (N) usage and how this was affected by the interactions between the poplar and understory vegetation. The results showed a generally lower periodic growth, and a significant decline in the foliar chlorophyll content and glutamine synthetase activity of poplar under treatment with RS and RD compared to those under UR treatment conducted in July 2019, which clearly indicated N competition between the understory vegetation and poplar trees. However, no significant difference was detected in the foliar chlorophyll content and glutamine synthetase activity of poplar under RD and RS treatment; only the nitrate reductase activity in poplar leaves under RD treatment declined significantly, by 22.25%, in June 2019. On the contrary, the diameter at breast height (DBH) of the poplar under RD treatment showed an increase of 34.69% from July to August 2019, compared with that under RS treatment. Furthermore, the increase in the species richness of understory vegetation resulted in an increase in the δ15N values in the poplar leaves, which was strongly regulated by the NH4+-N pool in the 10–20 cm soil layer, indicating the effective coordination of N utilization between poplar and understory vegetation when diversified understory plant species were present. These findings demonstrate the essential role of understory vegetation species diversity in alleviating N competition with crop trees, and provide guidance for understory vegetation management in poplar plantations.
A Comprehensive Evaluation Model for Wood Companies Websites Based on the AHP/R-TOPSIS Method
Authors: Kropivšek Jože , Grošelj Petra , Oblak Leon , Jošt Matej
Published: May 2021

Recently, partly as a result of the COVID-19 pandemic, companies are increasingly shifting their activities to the Internet environment, thus accelerating the growth of online shopping. The aim of this study was to develop a comprehensive model for the evaluation of websites of wood companies, as a review of the literature shows that such a model does not yet exist. In developing the model, we used an innovative approach and combined the analytic hierarchy process with R-TOPSIS (technique for order of preference by similarity to ideal solution), which is a novelty in this field. For the final website assessment, the Website Quality Index (WQI) was developed. The main difference to other indexes is that our model has a comprehensive content background (36 criteria and sub-criteria) and a strong mathematical basis, so that it can be used to independently evaluate a single website or to evaluate a larger group of websites from the same industry. We have tested the model on 60 websites of Slovenian wood industry companies with at least ten employees. The results show the low to moderate average quality of the websites, with an average WQI of all evaluated websites of 0.450, indicating only an acceptable website. It has proven to be easy to use, and the results show the reliability of the website rating. Additionally, the designed model is an important tool for industry website developers and the basis for future studies in this field. However, it can also be applied to other areas related to wood and wood products, where the performance of objects should be evaluated with respect to multiple criteria.
Post-Fire Impacts of Vegetation Burning on Soil Properties and Water Repellency in a Pine Forest, South Korea
Authors: Li Qiwen , Ahn Sujung , Kim Taehyun , Im Sangjun
Published: May 2021

Forest fires can have a direct and immediate impact on soil properties, particularly soil water repellency. This study investigated the direct impacts of the Gangneung forest fire of 2019 on soil properties and the spatial variability of soil water repellency with vegetation burn severity in the Korean red pine (Pinus densiflora Siebold and Zucc) forest of South Korea. A total of 36 soil samples were collected at depth intervals of 0–5 cm, 10–15 cm, and 20–25 cm from three burned sites, representing surface-fuel consumption (SC), foliage necrosis (FN), and crown-fuel consumption (CC), respectively. An unburned site was also used as a control. Soil properties such as soil texture, pH, bulk density, electrical conductivity (EC), total organic carbon (TOC), and cation exchange capacity (CEC) were analyzed in the laboratory. The increase in the sand fraction near the soil surface after a fire was associated with changes in silt and clay fractions. Moderate to high vegetation burn severity at the FN and CC sites caused a decrease in soil pH due to the thermal destruction of kaolinite mineral structure, but organic matter combustion on the soil surface increased soil pH at the SC site. Forest fires led to increases in total organic carbon at the FN and SC sites, owing to the external input of heat damaged foliage and burnt materials. Molarity of an ethanol droplet (MED) tests were also conducted to measure the presence and intensity of soil water repellency from different locations and soil depths. MED tests showed that vegetation burn severity was important for determining the strength of water repellency, because severely burned sites tended to have stronger water repellency of soil than slightly burned sites. Unburned soils had very hydrophilic characteristics across soil depths, but a considerably thick hydrophobic layer was found in severely burned sites. The soil water repellency tended to be stronger on steep (>30°) slopes than on gentle (<15°) slopes.
Allocation of Wood Density in European Oak (Quercus robur L.) Trees Grown under a Canopy of Scots Pine
Authors: Jakubowski Marcin , Dobroczyński Marek
Published: May 2021

The allocation of wood density in trees depends on many factors, but mainly on the tree species. A great number of studies have analysed wood density in dominant or codominant trees, but only a few have focused on trees grown under canopy. We examined the basic wood density and fresh wood density of natural origin oaks growing under canopy of artificially planted Scots pine. The major purpose of the work was to study the wood density allocation in different parts of the trees such as the trunk and branches. From a total of 80 oaks we selected eight model trees and measured biometric features of their trunks and crowns. Wood samples from different parts of the trunk and crown were collected after the trees were felled. We observed significant differences between the average basic wood density (595 kg·m−3) and the average fresh wood density (1031 kg·m−3). The central part of the trunk and heartwood shows much higher density than the outer part of the trunk and sapwood, which corresponds to the model of ring-porous trees. Both types of wood density (basic and fresh) were also higher in the trunk than in the branches. The wood density of the branches differed between two zones: A1, which was closer to the trunk and had higher density; and A2, which was farther away from the trunk with lower density. Wood density shows positive correlation with crown length but not with crown width, which was more connected with diameter at breast height. We found lower value of slenderness than the value reported by other authors in oaks planted without canopy. The allocation of wood density in trees is associated with the potential mechanical load.
Titanium Trisulfide Nanoribbons Affect the Downy Birch and Poplar × Aspen Hybrid in Plant Tissue Culture via the Emission of Hydrogen Sulfide
Authors: Zakharova Olga V. , Gusev Alexander A. , Muratov Dmitry S. , Shuklinov Alexey V. , Strekalova Nataliya S. , Matveev Sergey M.
Published: May 2021

Plant tissue culture is an important tool for accelerated vegetative reproduction of woody plants. To increase the efficiency of this method, it is necessary to search for new growth stimulators and protectors of microshoots. Two-dimensional (2D) nanomaterials are highly promising for applications in medicine and biotechnologies. We have studied the effects of TiS3 nanoribbons with the following mean dimensions: thickness less than 100 nm, length 1–10 μm and width 0.4–1 μm upon poplar × aspen hybrid and downy birch microclones in plant tissue culture. We have found the effectiveness of this particular nanomaterial as a sterilizing and stimulating agent in the initial growth stage and as a rhizogenesis-activating agent in the rooting stage. We analyzed a wide range of TiS3 concentrations in the nutrient media and identified 1.5 and 3 μg/L as optimal. However, concentration-dependent toxic effects manifesting themselves through microclones viability suppression have been discovered in the groups exposed to 6 and 15 μg/L TiS3. We have established that plant reaction to TiS3 treatment is specific for different plant species, i.e., downy birch is generally more tolerant than poplar × aspen hybrid. Here, we propose that the protective and stimulating effects of titanium trisulfide as well as its toxicity in high concentrations can be explained by the release of hydrogen sulfide as a result of aqueous hydrolysis of nanoribbons and its effect on plants. Additional studies are required in order to assess all biological effects produced by TiS3 nanoribbons at further stages of ontogenetic development and to identify the mechanisms of their action.
Nanopore Sequencing Unveils Diverse Transcript Variants of the Epithelial Cell-Specific Transcription Factor Elf-3 in Human Malignancies
Authors: Boti Michaela A. , Adamopoulos Panagiotis G. , Tsiakanikas Panagiotis , Scorilas Andreas
Published: May 2021

The human E74-like ETS transcription factor 3 (Elf-3) is an epithelium-specific member of the ETS family, all members of which are characterized by a highly conserved DNA-binding domain. Elf-3 plays a crucial role in epithelial cell differentiation by participating in morphogenesis and terminal differentiation of the murine small intestinal epithelium, and also acts as an indispensable regulator of mesenchymal to epithelial transition, underlying its significant involvement in development and in pathological states, such as cancer. Although previous research works have deciphered the functional role of Elf-3 in normal physiology as well as in tumorigenesis, the present study highlights for the first time the wide spectrum of ELF3 mRNAs that are transcribed, providing an in-depth analysis of splicing events and exon/intron boundaries in a broad panel of human cell lines. The implementation of a versatile targeted nanopore sequencing approach led to the identification of 25 novel ELF3 mRNA transcript variants (ELF3 v.3–v.27) with new alternative splicing events, as well as two novel exons. Although the current study provides a qualitative transcriptional profile regarding ELF3, further studies must be conducted, so the biological function of all novel alternative transcript variants as well as the putative protein isoforms are elucidated.
Transcriptome Profiling of Embryonic Retinal Pigment Epithelium Reprogramming
Authors: Tangeman Jared A. , Luz-Madrigal Agustín , Sreeskandarajan Sutharzan , Grajales-Esquivel Erika , Liu Lin , Liang Chun , Tsonis Panagiotis A. , Del Rio-Tsonis Katia
Published: May 2021

The plasticity of human retinal pigment epithelium (RPE) has been observed during proliferative vitreoretinopathy, a defective repair process during which injured RPE gives rise to fibrosis. In contrast, following injury, the RPE of the embryonic chicken can be reprogrammed to regenerate neural retina in a fibroblast growth factor 2 (FGF2)-dependent manner. To better explore the mechanisms underlying embryonic RPE reprogramming, we used laser capture microdissection to isolate RNA from (1) intact RPE, (2) transiently reprogrammed RPE (t-rRPE) 6 h post-retinectomy, and (3) reprogrammed RPE (rRPE) 6 h post-retinectomy with FGF2 treatment. Using RNA-seq, we observed the acute repression of genes related to cell cycle progression in the injured t-rRPE, as well as up-regulation of genes associated with injury. In contrast, the rRPE was strongly enriched for mitogen-activated protein kinase (MAPK)-responsive genes and retina development factors, confirming that FGF2 and the downstream MAPK cascade are the main drivers of embryonic RPE reprogramming. Clustering and pathway enrichment analysis was used to create an integrated network of the core processes associated with RPE reprogramming, including key terms pertaining to injury response, migration, actin dynamics, and cell cycle progression. Finally, we employed gene set enrichment analysis to suggest a previously uncovered role for epithelial-mesenchymal transition (EMT) machinery in the initiation of embryonic chick RPE reprogramming. The EMT program is accompanied by extensive, coordinated regulation of extracellular matrix (ECM) associated factors, and these observations together suggest an early role for ECM and EMT-like dynamics during reprogramming. Our study provides for the first time an in-depth transcriptomic analysis of embryonic RPE reprogramming and will prove useful in guiding future efforts to understand proliferative disorders of the RPE and to promote retinal regeneration.
Malaria in the ‘Omics Era’
Authors: Pegoraro Mirko , Weedall Gareth D.
Published: May 2021

Genomics has revolutionised the study of the biology of parasitic diseases. The first Eukaryotic parasite to have its genome sequenced was the malaria parasite Plasmodium falciparum. Since then, Plasmodium genomics has continued to lead the way in the study of the genome biology of parasites, both in breadth—the number of Plasmodium species’ genomes sequenced—and in depth—massive-scale genome re-sequencing of several key species. Here, we review some of the insights into the biology, evolution and population genetics of Plasmodium gained from genome sequencing, and look at potential new avenues in the future genome-scale study of its biology.
Geochemical Characterization and Trace-Element Mobility Assessment for Metallic Mine Reclamation in Soils Affected by Mine Activities in the Iberian Pyrite Belt
Authors: Sánchez-Donoso Ramón , García Lorenzo Mari Luz , Esbrí José María , García-Noguero Eva María , Higueras Pablo , Crespo Elena
Published: May 2021

The geochemical characterization of the mine deposits and soils in metal mining areas is essential in order to develop an effective mine reclamation strategy. The determination of total potentially toxic element (PTE) content, together with the application of chemical extraction procedures, can give insight into the behavior of contaminants after the application of different mine reclamation solutions, as well as identify the areas where urgent action is needed. This work presents a practical application to the evaluation of the pollution potential of trace elements in soils affected by mining activities, to be used in metallic mine reclamation. The PTE behavior was assessed by single extractions in order to simulate four environmental conditions: PTE mobility under rainfall conditions, acid mine drainage, reducing conditions, and plant uptake. The spatial distribution of contaminants in the study area was evaluated by determination of PTE total content in soil samples. Trace elements with high natural mobility, such as Zn, appeared concentrated at water and sediment discharge areas, while As, Pb, and Cu contents were higher near the mine wastes. The results obtained after the extractions suggested that the highest PTE content was extracted in the complexing–reducing medium, due to the dissolution of secondary sulfates and Fe3+ oxyhydroxides and the subsequent release of PTEs associated with those mineral phases. Reclamation strategies applied in the study area should promote efficient water drainage, infiltration, and subsuperficial water circulation in order to maintain oxidant conditions in the soil. The methodology applied in this study may constitute a valuable tool to define the geochemical constraints in metal mining areas, as well as help to develop appropriate mine reclamation solutions.
Efficiency of Intensity Measures Considering Near- and Far-Fault Ground Motion Records
Authors: Vargas-Alzate Yeudy , Hurtado Jorge
Published: May 2021

This paper focuses on the identification of high-efficiency intensity measures to predict the seismic response of buildings affected by near- and far-fault ground motion records. Near-fault ground motion has received special attention, as it tends to increase the expected damage to civil structures compared to that from ruptures originating further afield. In order to verify this tendency, the nonlinear dynamic response of 3D multi-degree-of-freedom models is estimated by using a subset of records whose distance to the epicenter is lower than 10 km. In addition, to quantify how much the expected demand may increase because of the proximity to the fault, another subset of records, whose distance to the epicenter is in the range between 10 and 30 km, has been analyzed. Then, spectral and energy-based intensity measures as well as those obtained from specific computations of the ground motion record are calculated and correlated to several engineering demand parameters. From these analyses, fragility curves are derived and compared for both subsets of records. It has been observed that the subset of records nearer to the fault tends to produce fragility functions with higher probabilities of exceedance than the ones derived for far-fault records. Results also show that the efficiency of the intensity measures is similar for both subsets of records, but it varies depending on the engineering demand parameter to be predicted.
Application of Green Nanoemulsion for Elimination of Rifampicin from a Bulk Aqueous Solution
Authors: Hussain Afzal , Mahdi Wael A. , Alshehri Sultan , Bukhari Sarah I. , Almaniea Mohammad A.
Published: May 2021

The study aimed to prepare green nanoemulsion (GNE) multi-components ((water/dimethyl sulfoxide–transcutol/isopropyl alcohol/capmul MCM C8 (CMC8)) to remove rifampicin (RIF) from a contaminated aqueous bulk solution. Pseudo ternary phase diagrams dictated several batches of GNE prepared following the reported method. Selected nanoemulsions (NF1–NF5) were characterized for morphology, globular size, size distribution (polydispersity index, PDI), viscosity, zeta potential, refractive index (RI), and free-thaw kinetic stability. They were investigated for percent removal efficiency (%RE) of RIF from the bulk aqueous solution for varied time intervals (10–60 min). Finally, scanning electron microscopy–energy dispersive x-ray (SEM–EDX) and inductive coupled plasma–optical emission system (ICP–OE) were used to confirm the extraction of trace content of dimethyl sulfoxide (DMSO) and others in the treated water. Considering the data obtained for globule size, PDI, viscosity, zeta potential, freeze–thaw stability, and refractive index, NF5 was the most suitable for RIF removal. The largest %RE value (91.7%) was related to NF5, which may be prudent to correlate with the lowest value (~39 nm) of size (maximum surface area available for contact adsorption), PDI (0.112), and viscosity (82 cP). Moreover, %RE was profoundly influenced by the content of CMC8 and the aqueous phase. These two phases had immense impact on the viscosity, size, and RI. The percent content of water, Smix, and CMC8 were 15% w/w), 60% w/w, and 25% w/w, respectively in NF5. SEM–EDX and ICP–OE confirmed the absence of DMSO and other hydrophilic components in the treated water. Thus, efficient NF5 could be a promising option to the conventional method to decontaminate the polluted aqueous system.
Automated Detection of Hypertension Using Physiological Signals: A Review
Authors: Sharma Manish , Rajput Jaypal Singh , Tan Ru San , Acharya U. Rajendra
Published: May 2021

Arterial hypertension (HT) is a chronic condition of elevated blood pressure (BP), which may cause increased incidence of cardiovascular disease, stroke, kidney failure and mortality. If the HT is diagnosed early, effective treatment can control the BP and avert adverse outcomes. Physiological signals like electrocardiography (ECG), photoplethysmography (PPG), heart rate variability (HRV), and ballistocardiography (BCG) can be used to monitor health status but are not directly correlated with BP measurements. The manual detection of HT using these physiological signals is time consuming and prone to human errors. Hence, many computer-aided diagnosis systems have been developed. This paper is a systematic review of studies conducted on the automated detection of HT using ECG, HRV, PPG and BCG signals. In this review, we have identified 23 studies out of 250 screened papers, which fulfilled our eligibility criteria. Details of the study methods, physiological signal studied, database used, various nonlinear techniques employed, feature extraction, and diagnostic performance parameters are discussed. The machine learning and deep learning based methods based on ECG and HRV signals have yielded the best performance and can be used for the development of computer-aided diagnosis of HT. This work provides insights that may be useful for the development of wearable for continuous cuffless remote monitoring of BP based on ECG and HRV signals.
The Role of Neuropsychological Factors in Perceived Threat of SARS-CoV-2 in Healthy Ageing
Authors: Bartoli Massimo , Canessa Nicola , Cipriani Giuseppina Elena , Cappa Stefano F. , Amanzio Martina
Published: May 2021

The COVID-19 pandemic is known to increase older adults’ vulnerability to adverse outcomes. Alongside increased physical frailty, anxiety symptoms associated with the risk of SARS-CoV-2 contagion appear to represent its most prominent ‘sequelae’. The attentional and linguistic resources required for decoding virus-related information may also influence the perceived threat of contagion. However, the possible role of neuropsychogeriatric factors on the latter dimension has never been assessed in a longitudinal study on the older population. To fill this gap, 50 healthy cognitively preserved older adults underwent a neuropsychological and physical frailty assessment before the pandemic (T0). Subsequently, they agreed to be interviewed and re-assessed during the lockdown (T1) and immediately after it (T2) through a longitudinal one-year study. Perceived threat of SARS-CoV-2 at T2 was predicted both by baseline anxiety and frailty scores, and by decreased performance in information processing speed and language comprehension tests. While confirming the joint role of frailty and anxiety, a moderation/interaction model showed that each of them was sufficient, at its highest level, to support the maximum degree of perceived threat of contagion. The contribution of neuropsychological factors to perceived threat of SARS-CoV-2 highlights their importance of tailoring information campaigns addressed to older people.
Association between Green Space Structure and the Prevalence of Asthma: A Case Study of Toronto
Authors: Dong Yuping , Liu Helin , Zheng Tianming
Published: May 2021

Asthma is a chronic inflammatory disease that can be caused by various factors, such as asthma-related genes, lifestyle, and air pollution, and it can result in adverse impacts on asthmatics’ mental health and quality of life. Hence, asthma issues have been widely studied, mainly from demographic, socioeconomic, and genetic perspectives. Although it is becoming increasingly clear that asthma is likely influenced by green spaces, the underlying mechanisms are still unclear and inconsistent. Moreover, green space influences the prevalence of asthma concurrently in multiple ways, but most existing studies have explored only one pathway or a partial pathway, rather than the multi-pathways. Compared to greenness (measured by Normalized Difference Vegetation Index, tree density, etc.), green space structure—which has the potential to impact the concentration of air pollution and microbial diversity—is still less investigated in studies on the influence of green space on asthma. Given this research gap, this research took Toronto, Canada, as a case study to explore the two pathways between green space structure and the prevalence of asthma based on controlling the related covariates. Using regression analysis, it was found that green space structure can protect those aged 0–19 years from a high risk of developing asthma, and this direct protective effect can be enhanced by high tree diversity. For adults, green space structure does not influence the prevalence of asthma unless moderated by tree diversity (a measurement of the richness and diversity of trees). However, this impact was not found in adult females. Moreover, the hypothesis that green space structure influences the prevalence of asthma by reducing air pollution was not confirmed in this study, which can be attributed to a variety of causes.
A Systematic Review and Meta-Analysis of Fall Prevention Programs for Pediatric Inpatients
Authors: Kim Eun-Joo , Kim Geun-Myun , Lim Ji-Young
Published: May 2021

Falls account for a high proportion of the safety accidents experienced by hospitalized children. This study aims to analyze the contents and effects of fall prevention programs for pediatric inpatients to develop more adaptable fall prevention programs. A literature search was performed using PubMed (including Medline), Science Direct, CINAHL, Embase, and Cochrane. We included articles published from the inception of each of the databases up to 31 March 2019. A total of 1725 results were reviewed according to the inclusion and exclusion criteria, and nine studies were selected. Data were analyzed using descriptive statistics and the Comprehensive Meta-Analysis program. Four of the nine studies divided their participants into a high-risk fall group and a low-or medium-risk fall group, and all studies used a high-risk sign/sticker as a common protocol guideline for its high-risk fall group. The odds ratio of 0.95 (95% Cl 0.550–1.640) for the fall prevention program in seven studies was not statistically significant. To develop a standardized fall prevention program in the future, randomized control trial studies that can objectively measure the fall rate reduction effect of the integrated fall prevention program need to be expanded.
The National Interscholastic Cycling Association (NICA) Mountain Biking Injury Surveillance System (ISS): Analysis of 66,588 Student Athlete-Years of Injury Data
Authors: Ehn Meredith , Teramoto Masaru , Cushman Daniel M. , Saad Kristen , Willick Stuart
Published: May 2021

Interscholastic youth cross-country mountain bike racing in the United States has grown significantly over the past decade, yet little is known about the risk profile in this age group. Aiming to protect participants, we implemented a prospective, longitudinal injury surveillance system for the purpose of better understanding youth mountain biking injuries and implementing safety measures. Data were collected during competition years 2018–2020, totaling 66,588 student athlete-years. Designated reporters from each team received weekly emails with exposure and incident report forms. Variables analyzed included demographic, rider-related, trail-related, and other data. Injury characteristics during the COVID-19 pandemic in 2020 were compared to the years 2018 and 2019. More student athletes participated in the 2020 season (25,261) than in prior seasons (18,575 in 2018 and 22,752 in 2019). During competition year 2020, overall injury proportion was lower (1.7% versus 3.0% in 2018 and 2.7% in 2019). Variables associated with injury, body part injured, type of injury, time-loss, and disposition following injury were similar between all years. Despite the pandemic and resultant changes to competition, student athletes continued to ride their bikes and become injured, but the proportion of injuries differed. This report details injury characteristics in youth mountain bike racing, including a comparison of before and during the pandemic.
Prevalence and Correlates of Prenatal Depression, Anxiety and Suicidal Behaviours in the Volta Region of Ghana
Authors: Kugbey Nuworza , Ayanore Martin , Doegah Phidelia , Chirwa Masauso , Bartels Susan A. , Davison Colleen M. , Purkey Eva
Published: May 2021

Pregnancy is associated with several physical and psychosocial challenges that influence women’s health and wellbeing. However, prenatal mental health has received little attention. Therefore, this study examined the prevalence and correlates of prenatal depression, anxiety and current suicidal behaviors among pregnant women in the Volta Region of Ghana. Two hundred and fourteen (n = 214) pregnant women recruited from two hospitals responded to the hospital depression and anxiety scale (HADS), the insomnia severity index, and a set of psycho-behavioral, socioenvironmental and demographic characteristic questions. Chi-squared, bivariate and multivariate logistic regression were used for data analysis. Prevalence of prenatal depression, anxiety and current suicidal behaviors was 50.5%, 35.5% and 3.3%, respectively. After controlling for other variables, average monthly income, insomnia, non-nutritious food consumption (pica), and body image satisfaction were significantly associated with depression. Marital status, insomnia, lifetime suicidal behavior and partner support were significantly associated with anxiety. Current partner abuse was the only factor significantly associated with current suicidal behavior. The high prevalence rates of anxiety and depression among pregnant women and intimate partner violence remain important maternal health issues in the region. Therefore, brief mental health screening and counseling services should be integrated into prenatal healthcare services.
Sleep Quality in Chilean Professional Soccer Players
Authors: Jorquera-Aguilera Carlos , Barahona-Fuentes Guillermo , Pérez Peña María José , Yeomans Cabrera María Mercedes , Huerta Ojeda Álvaro
Published: May 2021

Recent research has shown that good sleep quality has a positive effect on physical performance. However, sleep quality in Chilean professional soccer players is unknown. The purpose of this study was to determine sleep quality in Chilean professional soccer players. It was a cross-sectional, explanatory study with observable variables. The sample consisted of 94 Chilean male soccer players belonging to four professional clubs. The main variable was the Sleep Quality Index, evaluated through the Pittsburgh questionnaire (Spanish version). After estimating sleep quality individually, the four professional soccer clubs’ comparison was performed through a one-factor ANOVA. The Pearson test was used to relate the questionnaire variables; the significance level was p < 0.05. In the global analysis of the Pittsburgh Sleep Quality Index, a value of 4.75 ± 2.29 on a scale of 0–21 was observed, with no significant differences between the clubs evaluated (p > 0.05). Based on the results obtained, Chilean male professional soccer players present good sleep quality. However, the high values of “sleep latency” and “sleep disturbances” are indicators that should be worked on by the multidisciplinary team of each professional club. They should develop strategies to improve sleep hygiene, encourage good sleep, and fall asleep efficiently.
Prevalence of Type 2 Diabetes in South Africa: A Systematic Review and Meta-Analysis
Authors: Pheiffer Carmen , Pillay-van Wyk Victoria , Turawa Eunice , Levitt Naomi , Kengne Andre P. , Bradshaw Debbie
Published: May 2021

Synthesis of existing prevalence data using rigorous systematic review methods is considered an effective strategy to generate representative and robust prevalence figures to inform health planning and policy. The purpose of this systematic review was to identify, collate, and synthesise all studies reporting the prevalence of total and newly diagnosed type 2 diabetes (T2DM), impaired glucose tolerance (IGT), and impaired fasting glucose (IFG) in South Africa. Four databases, PubMed, Scopus, Web of Science, and African Index Medicus were searched for articles published between January 1997 and June 2020. A total of 1886 articles were identified, of which 11 were included in the meta-analysis. The pooled prevalence in individuals 25 years and older was 15.25% (11.07–19.95%) for T2DM, 9.59% (5.82–14.17%) for IGT, 3.55% (0.38–9.61%) for IFG, and 8.29% (4.97–12.34%) for newly diagnosed T2DM. Although our pooled estimate may be imprecise due to significant heterogeneity across studies with regard to population group, age, gender, setting, diagnostic test, and study design, we provide evidence that the burden of glucose intolerance in South Africa is high. These factors contribute to the paucity of representative T2DM prevalence data. There is a need for well-designed epidemiological studies that use best-practice and standardised methods to assess prevalence.
The Effect of Labor and Relationship Exclusions on Older Korean Men with Depression
Authors: Kwon Eun-Jung , Jung Hye-Sun
Published: May 2021

To manage depression of older men, it is necessary to identify factors that influence depression and improve them. This study used the Korean Aging Longitude Research to understand the effects of labor exclusion and relationship exclusion on depression in Korean male seniors aged 65 and older. According to research on the effect of labor exclusion and relationship exclusion on depression, depression in the case where labor was excluded was 1.69 times higher in 2014, 1.65 times higher in 2016, and 1.93 times higher in 2018 compared to the case where labor was not excluded. Depression in the case where relationship was excluded was 2.94 times higher in 2014, 3.15 times higher in 2016, and 2.57 times higher in 2018 compared to the case where relationship was not excluded. Depression in the case where labor and relationship were both excluded was 3.00 times higher in 2014, 3.23 times higher in 2016, and 2.81 times higher in 2018 compared to the case where neither labor nor relationship was excluded. Since labor exclusion and relationship exclusion have a big influence on depression in older men, it is necessary to establish plans for job creation and for the formation of social relationships for the elderly.
Quieted City Sounds during the COVID-19 Pandemic in Montreal
Authors: Steele Daniel , Guastavino Catherine
Published: May 2021

This paper investigates the transformation of urban sound environments during the COVID-19 pandemic in Montreal, Canada. We report on comparisons of sound environments in three sites, before, during, and after the lockdown. The project is conducted in collaboration with the Montreal festival district (Quartier des Spectacles) as part of the Sounds in the City partnership. The analyses rely on continuous acoustic monitoring of three sites. The comparisons are presented in terms of (1) energetic acoustic indicators over different periods of time (Lden, Ld, Le, Ln), (2) statistical acoustic indicators (L10, L90), and (3) hourly, daily, and weekly profiles of sound levels throughout the day. Preliminary analyses reveal sound level reductions on the order of 6–7 dB(A) during lockdown, with differences more or less marked across sites and times of the day. After lockdown, sound levels gradually increased following an incremental relaxation of confinement. Within four weeks, sound levels measurements nearly reached the pre-COVID-19 levels despite a reduced number of pedestrian activities. Long-term measurements suggest a ‘new normal’ that is not quite as loud without festival activities, but that is also not characterizable as quiet. The study supports reframing debates about noise control and noise management of festival areas to also consider the sounds of such areas when festival sounds are not present.
Malaria Infection and Risk for Endemic Burkitt Lymphoma: A Systematic Review and Meta-Analysis
Authors: Kotepui Kwuntida Uthaisar , Kotepui Manas
Published: May 2021

Background: Malaria infection is reportedly linked to endemic Burkitt lymphoma (eBL) in malaria-endemic areas. This study aimed to pool the overall risk (or odds) of eBL among children with previous or concurrent malaria infection. Methods: We searched PubMed, Web of Science, Scopus, and reference lists of publications for potentially relevant studies on malaria infection and eBL. The quality of the included studies was assessed using the Joanna Briggs Institute for case-control studies. Random-effects meta-analysis was used to summarize whether the odds of eBL can be increased by (1) malaria infection or (2) elevated titer of IgGs to malaria antigen. The level of heterogeneity was evaluated using Cochran’s Q statistic and I2. The individual study data, pooled odds, and confidence interval (CI) were illustrated using the forest plot. Publication bias was assessed using funnel plots and Egger’s test. Results: Ten studies were included, reporting the number of malaria cases in eBL and non-eBL (5 studied malaria infection and the odds of eBL; five studied the burden of IgGs to malarial antigens and the odds of eBL). According to the meta-analysis results, the odds of eBL was not increased by malaria infection (p = 0.562, OR: 0.87, 95% CI: 0.54–1.39, I2: 93.5%, malaria in eBL: 604/1506 cases, malaria in non-eBL: 2117/4549 cases) and the elevated titer of IgGs to malaria antigen (p = 0.051, OR: 1.50, 95% CI: 1.00–2.25, I2: 89%, increased IgG titer in eBL: 1059/1736 cases, increased IgG titer in non-eBL: 847/1722 cases). In meta-regression analysis, sex was not a confounding factor for the effect size of malaria infection and eBL (p = 0.10) and that of increased IgGs and eBL (p = 0.80). Conclusions: Malaria infection and IgG titer elevation did not increase the risk for eBL among children. However, the included studies, which are only few, do not generally agree on this point. Therefore, the risk for eBL in children diagnosed with malaria should be investigated further by longitudinal studies to confirm our evidence-based approach.
Implementing Public Health Strategies—The Need for Educational Initiatives: A Systematic Review
Authors: Khorram-Manesh Amir , Dulebenets Maxim A. , Goniewicz Krzysztof
Published: May 2021

In the absence of a specific treatment or vaccines, public health strategies are the main measures to use in the initial stages of a pandemic to allow surveillance of infectious diseases. During the ongoing global pandemic of coronavirus disease 2019 (COVID-19), several countries initiated various public health strategies, such as contact tracing and quarantine. The present study aims to conduct a systematic literature review to identify the presence of educational initiatives that promote the implementation of public health strategies before public health emergencies, with a special focus on contact tracing applications. Using Science Direct, PubMed, Scopus, and Gothenburg University search engines, all published scientific articles were included, while conference, reports, and non-scientific papers were excluded. The outcomes of the reviewed studies indicate that the effective implementation of public health strategies depends on the peoples’ willingness to participate and collaborate with local authorities. Several factors may influence such willingness, of which ethical, psychological, and practical factors seem to be the most important and frequently discussed. Moreover, individual willingness and readiness of a community may also vary based on the acquired level of knowledge about the incident and its cause and available management options. Educational initiatives, proper communication, and timely information at the community level were found to be the necessary steps to counteract misinformation and to promote a successful implementation of public health strategies and attenuate the effects of a pandemic. The systematic review conducted as a part of this study would benefit the relevant stakeholders and policy makers and assist with effective designing and implementation.
Effect of Epinephrine Administered during Cardiopulmonary Resuscitation on Cerebral Oxygenation after Restoration of Spontaneous Circulation in a Swine Model with a Clinically Relevant Duration of Untreated Cardiac Arrest
Authors: Lee Hyoung Youn , Shamsiev Kamoljon , Mamadjonov Najmiddin , Jung Yong Hun , Jeung Kyung Woon , Kim Jin Woong , Heo Tag , Min Yong Il
Published: May 2021

Severe neurological impairment was more prevalent in cardiac arrest survivors who were administered epinephrine than in those administered placebo in a randomized clinical trial; short-term reduction of brain tissue O2 tension (PbtO2) after epinephrine administration in swine following a short duration of untreated cardiac arrest has also been reported. We investigated the effects of epinephrine administered during cardiopulmonary resuscitation (CPR) on cerebral oxygenation after restoration of spontaneous circulation (ROSC) in a swine model with a clinically relevant duration of untreated cardiac arrest. After 7 min of ventricular fibrillation, 24 pigs randomly received either epinephrine or saline placebo during CPR. Parietal cortex measurements during 60-min post-resuscitation period showed that the area under the curve (AUC) for PbtO2 was smaller in the epinephrine group than in the placebo group during the initial 10-min period and subsequent 50-min period (both p < 0.05). The AUC for number of perfused cerebral capillaries was smaller in the epinephrine group during the initial 10-min period (p = 0.005), but not during the subsequent 50-min period. In conclusion, epinephrine administered during CPR reduced PbtO2 for longer than 10 min following ROSC in a swine model with a clinically relevant duration of untreated cardiac arrest.
Assessment of Influencing Factors on the Spatial Variability of SOM in the Red Beds of the Nanxiong Basin of China, Using GIS and Geo-Statistical Methods
Authors: Yan Ping , Lin Kairong , Wang Yiren , Tu Xinjun , Bai Chunmei , Yan Luobin
Published: May 2021

Understanding the spatial variability of soil organic matter (SOM) is crucial for implementing precise land degradation control and fertilization to improve crop productivity. Studying spatial variability provides a scientific basis for precision fertilization and land degradation control. In this study, geostatistics and classical statistical methods were used to analyze the spatial variability of SOM and its influencing factors under various degrees of land degradation in the red bed area of southern China. The results demonstrate a declining trend for SOM content with increasing land degradation. The SOM content differs profoundly under different land degradation degrees. The coefficient of variation ranges from 13.61% for extreme land degradation to 8.98% for mild land degradation, 7.96% for moderate land degradation, and 5.64% for severe land degradation. A significant positive correlation is displayed between the altitude and the SOM (p < 0.01) under mild and moderate land degradation conditions. Bulk density and pH value have a significant negative correlation with SOM (p < 0.01). It can be observed that terrain factors, as well as physical and chemical soil parameters, have a great influence on SOM.
VirtualFlow Ants—Ultra-Large Virtual Screenings with Artificial Intelligence Driven Docking Algorithm Based on Ant Colony Optimization
Authors: Gorgulla Christoph , Çınaroğlu Süleyman Selim , Fischer Patrick D. , Fackeldey Konstantin , Wagner Gerhard , Arthanari Haribabu
Published: May 2021

The docking program PLANTS, which is based on ant colony optimization (ACO) algorithm, has many advanced features for molecular docking. Among them are multiple scoring functions, the possibility to model explicit displaceable water molecules, and the inclusion of experimental constraints. Here, we add support of PLANTS to VirtualFlow (VirtualFlow Ants), which adds a valuable method for primary virtual screenings and rescoring procedures. Furthermore, we have added support of ligand libraries in the MOL2 format, as well as on the fly conversion of ligand libraries which are in the PDBQT format to the MOL2 format to endow VirtualFlow Ants with an increased flexibility regarding the ligand libraries. The on the fly conversion is carried out with Open Babel and the program SPORES. We applied VirtualFlow Ants to a test system involving KEAP1 on the Google Cloud up to 128,000 CPUs, and the observed scaling behavior is approximately linear. Furthermore, we have adjusted several central docking parameters of PLANTS (such as the speed parameter or the number of ants) and screened 10 million compounds for each of the 10 resulting docking scenarios. We analyzed their docking scores and average docking times, which are key factors in virtual screenings. The possibility of carrying out ultra-large virtual screening with PLANTS via VirtualFlow Ants opens new avenues in computational drug discovery.
Connexin 43 and Connexin 26 Involvement in the Ponatinib-Induced Cardiomyopathy: Sex-Related Differences in a Murine Model
Authors: Madonna Rosalinda , Moscato Stefania , Polizzi Enza , Pieragostino Damiana , Cufaro Maria Concetta , Del Boccio Piero , Bianchi Francesco , De Caterina Raffaele , Mattii Letizia
Published: May 2021

Cardiac connexins (Cxs) are proteins responsible for proper heart function. They form gap junctions that mediate electrical and chemical signalling throughout the cardiac system, and thus enable a synchronized contraction. Connexins can also individually participate in many signal transduction pathways, interacting with intracellular proteins at various cellular compartments. Altered connexin expression and localization have been described in diseased myocardium and the aim of this study is to assess the involvement of Cx43, Cx26, and some related molecules in ponatinib-induced cardiac toxicity. Ponatinib is a new multi-tyrosine kinase inhibitor that has been successfully used against human malignancies, but its cardiotoxicity remains worrisome. Therefore, understanding its signaling mechanism is important to adopt potential anti cardiac damage strategies. Our experiments were performed on hearts from male and female mice treated with ponatinib and with ponatinib plus siRNA-Notch1 by using immunofluorescence, Western blotting, and proteomic analyses. The altered cardiac function and the change in Cxs expression observed in mice after ponatinib treatment, were results dependent on the Notch1 pathway and sex. Females showed a lower susceptibility to ponatinib than males. The downmodulation of cardiac Cx43, Cx26 and miR-122, high pS368-Cx43 phosphorylation, cell viability and survival activation could represent some of the female adaptative/compensatory reactions to ponatinib cardiotoxicity.