bluuebunny/crossref_metadata_2025 huggingface dataset:
An explorative study on respiratory health among operators working in polymer additive manufacturing
Authors: Almstrand Ann-Charlotte , Bredberg Anna , Runström Eden Gunilla , Karlsson Helen , Assenhöj Maria , Koca Hatice , Olin Anna-Carin , Tinnerberg Håkan
Published: April 2023

Additive manufacturing (AM), or 3D printing, is a growing industry involving a wide range of different techniques and materials. The potential toxicological effects of emissions produced in the process, involving both ultrafine particles and volatile organic compounds (VOCs), are unclear, and there are concerns regarding possible health implications among AM operators.

The objective of this study was to screen the presence of respiratory health effects among people working with liquid, powdered, or filament plastic materials in AM.

Methods

In total, 18 subjects working with different additive manufacturing techniques and production of filament with polymer feedstock and 20 controls participated in the study. Study subjects filled out a questionnaire and underwent blood and urine sampling, spirometry, impulse oscillometry (IOS), exhaled NO test (FeNO), and collection of particles in exhaled air (PEx), and the exposure was assessed. Analysis of exhaled particles included lung surfactant components such as surfactant protein A (SP-A) and phosphatidylcholines. SP-A and albumin were determined using ELISA. Using reversed-phase liquid chromatography and targeted mass spectrometry, the relative abundance of 15 species of phosphatidylcholine (PC) was determined in exhaled particles. The results were evaluated by univariate and multivariate statistical analyses (principal component analysis).

Results

Exposure and emission measurements in AM settings revealed a large variation in particle and VOC concentrations as well as the composition of VOCs, depending on the AM technique and feedstock. Levels of FeNO, IOS, and spirometry parameters were within clinical reference values for all AM operators. There was a difference in the relative abundance of saturated, notably dipalmitoylphosphatidylcholine (PC16:0_16:0), and unsaturated lung surfactant lipids in exhaled particles between controls and AM operators.

Conclusion

There were no statistically significant differences between AM operators and controls for the different health examinations, which may be due to the low number of participants. However, the observed difference in the PC lipid profile in exhaled particles indicates a possible impact of the exposure and could be used as possible early biomarkers of adverse effects in the airways.
Factors associated with sleep disorders among adolescent students in rural areas of China
Authors: Li Dan-Lin , Nie Xin-Yi , Li Jun , Tao Yi-Jin , Zhao Chun-Hua , Zhong Hua , Pan Chen-Wei
Published: April 2023

Background

This study aimed to determine sleep patterns and the prevalence and association factors of sleep disorders in a regionally representative sample in Mo Jiang, China.

Methods

A total of 2,346 (participation rate 93.5%) Grade 7 students (aged 13–14 years) from 10 middle schools, including 1,213 (51.7%) boys and 1,133 (48.3%) girls, participated in the study. All the participants were invited to complete questionnaires that acquired information on sleep patterns, academic performance, academic stress, and sociodemographic factors. Sleep disorders were assessed using the Chinese version of the Children’s Sleep Habits Questionnaire. Logistic regression models were used to investigate factors associated with sleep disorders.

Results

The prevalence of sleep disorders among rural adolescents was 76.4%, which is higher than that among urban adolescents. Compared with previous findings in urban areas, our results indicate that sleep loss is much more severe in rural adolescents. Sleep disorders were positively associated with factors, such as watching TV [odds ratio (OR) = 1.22, p = 0.001], academic performance (OR = 1.80, p < 0.001), and academic stress (OR = 1.38, p = 0.04). In addition, girls were more likely to suffer from sleep disorders than boys (OR = 1.36, p = 0.01).

Conclusion

Insufficient sleep and sleep disorders have become common health problems in rural Chinese adolescents.
Construction of smart older adults care service model driven by primary health care
Authors: Zhang Lechuan , Xu Xiaoyan
Published: April 2023

In recent years, with the arrival of an overall aging population, how to provide for the elderly has become the focus of government departments and society. The traditional elderly care service model has problems such as backward construction of information platforms, low quality of elderly care services, and “digital divide”. For this reason, based on grassroots medical and health care, this paper improves the quality of elderly care services by establishing a smart elderly care service model. Through experiments, it can be found that compared with the traditional elderly care service model, the intelligent elderly care service model has an absolute advantage in the identification of nursing data. The recognition accuracy rate of the smart elderly care service model for all kinds of daily care data is above 94%, while the recognition accuracy rate of the traditional elderly care service model is below 90%. Therefore, it is of great significance to study the smart elderly care service model driven by primary medical care and health.
Bicycle use in Latin American cities: changes over time by socio-economic position
Authors: Avila-Palencia Ione , Sarmiento Olga L. , Gouveia Nelson , Jáuregui Alejandra , Mascolli Maria A. , Slovic Anne D. , Rodríguez Daniel A.
Published: April 2023

Introduction

We aimed to examine utilitarian bicycle use among adults from 18 large Latin American cities and its association with socio-economic position (education and income) between 2008 and 2018.

Methods

Data came from yearly cross-sectional surveys collected by the Development Bank of Latin America (CAF). A total of 77,765 survey respondents with complete data were used to estimate multilevel logistic regression models with city as random intercept and year as random slope.

Results

Individuals with high education and high-income levels had lower odds of using a bicycle compared with participants with lower education and income levels. These associations, however, changed over time with the odds of bicycle use increasing for all groups, especially among individuals with the highest education and income levels.

Discussion

Our results confirm the broadening appeal of bicycling across socio-economic positions in several Latin American cities and reinforce the importance of considering policies aimed at supporting and enhancing bicycle travel for all users.
Combining computer aided food engineering and electro-heating applications as contribution to food processing sustainability
Authors: Marra Francesco
Published: April 2023

Can the combination of computer aided food engineering and electro-heating applications contribute to food processing sustainability? To what United Nations Sustainable Development Goal (SDG) this combination could contribute? Those are the questions addressed in this perspective. SDG7 is aimed to ensure the access to affordable, reliable, and sustainable and modern energy for all. According to World Bank, ensuring access to modern food cooking solutions is a key component to achieving for SDG7, and -since cooking is mainly based on heating processing- also the access to modern food heating solutions must be considered as a key component to achieving for SDG7. Electro-heating applications (EHA) in food processing include all processes using the interaction of a food material or product with an electromagnetic field, as in microwave, radio-frequency, and moderate electric fields processing. These technologies involve the use of electrical and -more recently- electronic circuits and require professional figures in their design. As result, the installation cost of these technologies is way higher than installation cost for heating processes using fossil sources, as fuel for fire boilers, as an example. Furthermore, at industrial level, these technologies may require ad-hoc design. Consequently, the food processing industry is quite slow in embracing such technologies along the productive lines. Computer aided food engineering (CAFE) has been recognized as valuable approach to shifts the paradigm from trial-and-error-based design to model-based design, using digital tools for the virtual representation of a food product or process, predicting the behavior of it. The combination of CAFE and EHA is the only way to help the food industry to embrace sustainability in most operations in which the heat transfer is involved. This paper presents a viewpoint on the challenges and opportunity to combine CAFE and EHAs as contribution to food processing sustainability.
Advances in lung bioengineering: Where we are, where we need to go, and how to get there
Authors: Hsiung Tiffany , James Les , Chang Stephanie H. , Geraci Travis C. , Angel Luis F. , Chan Justin C. Y.
Published: April 2023

Lung transplantation is the only potentially curative treatment for end-stage lung failure and successfully improves both long-term survival and quality of life. However, lung transplantation is limited by the shortage of suitable donor lungs. This discrepancy in organ supply and demand has prompted researchers to seek alternative therapies for end-stage lung failure. Tissue engineering (bioengineering) organs has become an attractive and promising avenue of research, allowing for the customized production of organs on demand, with potentially perfect biocompatibility. While breakthroughs in tissue engineering have shown feasibility in practice, they have also uncovered challenges in solid organ applications due to the need not only for structural support, but also vascular membrane integrity and gas exchange. This requires a complex engineered interaction of multiple cell types in precise anatomical locations. In this article, we discuss the process of creating bioengineered lungs and the challenges inherent therein. We summarize the relevant literature for selecting appropriate lung scaffolds, creating decellularization protocols, and using bioreactors. The development of completely artificial lung substitutes will also be reviewed. Lastly, we describe the state of current research, as well as future studies required for bioengineered lungs to become a realistic therapeutic modality for end-stage lung disease. Applications of bioengineering may allow for earlier intervention in end-stage lung disease and have the potential to not only halt organ failure, but also significantly reverse disease progression.
Wiser With Age? Understanding Aging Brains
Authors: Daley Ryan T. , Ford Jaclyn H. , Kensinger Elizabeth A.
Published: April 2023

Have you ever wondered if someone who is 72 uses their brain any differently than someone who is 27? What are the changes to the ways that our brains look and work as we grow older? What tasks get easier because of these changes, and which get harder? If you wonder about any of these things, keep reading—because these are the questions we will answer!
Illuminating the Living Lanterns of Antarctica
Authors: Belcher Anna , Freer Jennifer J. , Dornan Tracey , Saunders Ryan A.
Published: April 2023

Lanternfish are a relatively small but very abundant fish. They live deep in the ocean’s “twilight” zone where there is not much light. A unique community of lanternfish live in the Southern Ocean, where they are a key part of the Antarctic food web. Lanternfish also play an important role in moving carbon from the atmosphere into the deep ocean, where it is stored. In this article, we explain current knowledge on Southern Ocean lanternfish, including how they produce their own light! We will also tell you about some mysteries surrounding lanternfish that scientists are yet to solve.
Slowing down decay: biological clocks in personalized medicine
Authors: Pinel Clémence , Green Sara , Svendsen Mette N.
Published: April 2023

This article discusses so-called biological clocks. These technologies, based on aging biomarkers, trace and measure molecular changes in order to monitor individuals' “true” biological age against their chronological age. Drawing on the concept of decay, and building on ethnographic fieldwork in an academic laboratory and a commercial firm, we analyze the implications of the development and commercialization of biological clocks that can identify when decay is “out of tempo.” We show how the building of biological clocks rests on particular forms of knowing decay: In the academic laboratory, researchers focus on endo-processes of decay that are internal to the person, but when the technology moves to the market, the focus shifts as staff bracket decay as exo-processes, which are seen as resulting from a person's lifestyle. As the technology of biological clocks travels from the laboratory to the market of online testing of the consumer's biological age, we observe shifting visions of aging: from an inevitable trajectory of decline to a malleable and plastic one. While decay is an inevitable trajectory starting at birth and ending with death, the commercialization of biological clocks points to ways of stretching time between birth and death as individuals “optimize” their biological age through lifestyle changes. Regardless of admitted uncertainties about what is measured and the connection between maintenance and future health outcomes, the aging person is made responsible for their decaying body and for enacting maintenance to slow down decay. We show how the biological clock's way of “knowing” decay turns aging and its maintenance into a life-long concern and highlight the normative implications of framing decay as malleable and in need of intervention.
Gendered ethnic choice effects at the transition to upper secondary education in Switzerland
Authors: Glauser David , Becker Rolf
Published: April 2023

Positive ethnic choice effects, namely a higher likelihood of attending more demanding educational tracks among students of immigrant origin compared to their native peers, are observed in many countries. Immigrant optimism, and thus the striving for upward social mobility, is seen as a key mechanism for explaining ethnic choice effects. However, research on this topic often ignores gendered educational pathways and trajectories. Based on data from German-speaking Switzerland on two school-leaver cohorts, our interest is on whether ethnic choice effects are observable for both female and male students whose parents were born in the Balkans, Turkey or Portugal. In addition, we examine the extent to which aspirations contribute to explaining ethnic choice effects for both genders. To disentangle the direct effect of a migration background and the mediating effect of aspirations on educational attainment at upper secondary level, we apply the reformulated KHB method in our analyzes. Overall, our findings indicate that migrant women have made up ground on their native peers between the two school-leaving cohorts, contributing to the widening of the gender gap within the migrant group of interest. Of particular importance, however, is our finding that ethnic choice effects are observed only for men, while we do not observe any evidence of ethnic choice effects in the sample of women. Consistent with previous findings, our results show that aspirations mediate part of the ethnic choice effect. Our findings support the consideration that the room for ethnic choice effects is related to the proportion of young men and women striving for academic education, with gender differences in this regard being particularly pronounced in education systems with a high degree of vocational specificity.
Machine-Learning Approaches for the Discovery of Electrolyte Materials for Solid-State Lithium Batteries
Authors: Hu Shengyi , Huang Chun
Published: April 2023

Solid-state lithium batteries have attracted considerable research attention for their potential advantages over conventional liquid electrolyte lithium batteries. The discovery of lithium solid-state electrolytes (SSEs) is still undergoing to solve the remaining challenges, and machine learning (ML) approaches could potentially accelerate the process significantly. This review introduces common ML techniques employed in materials discovery and an overview of ML applications in lithium SSE discovery, with perspectives on the key issues and future outlooks.
Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
Authors: Seidel Stefan , Maschke Rüdiger W. , Mozaffari Fruhar , Eibl-Schindler Regine , Eibl Dieter
Published: April 2023

HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to determine the hydrodynamic stress in both shake flasks, with and without baffles, and in stirred Minifors 2 bioreactors to evaluate its effect on the growth and aggregate size distribution of HEK293 suspension cells. The HEK FreeStyleTM 293-F cell line was cultivated in batch mode at different specific power inputs (from 63 W m−3 to 451 W m−3), whereby ≈60 W m−3 corresponds to the upper limit, which is what has been typically described in published experiments. In addition to the specific growth rate and maximum viable cell density VCDmax, the cell size distribution over time and cluster size distribution were investigated. The VCDmax of (5.77±0.02)·106cellsmL−1 was reached at a specific power input of 233 W m−3 and was 23.8% higher than the value obtained at 63 W m−3 and 7.2% higher than the value obtained at 451 W m−3. No significant change in the cell size distribution could be measured in the investigated range. It was shown that the cell cluster size distribution follows a strict geometric distribution whose free parameter p is linearly dependent on the mean Kolmogorov length scale. Based on the performed experiments, it has been shown that by using CFD-characterised bioreactors, the VCDmax can be increased and the cell aggregate rate can be precisely controlled.
Intramuscular Polydeoxyribonucleotides in Fibrotic and Atrophic Localized Scleroderma: An Explorative Prospective Cohort Study
Authors: Romagnuolo Maurizio , Moltrasio Chiara , Marzano Angelo Valerio , Nazzaro Gianluca , Muratori Simona , Recalcati Sebastiano
Published: April 2023

Effective options in the quiescent, scantily inflammatory phase of localized scleroderma (morphea) are lacking. A cohort study in patients with histologically confirmed fibroatrophic morphea explored the therapeutic value of the anti-dystrophic A2A adenosine agonist polydeoxyribonucleotide (PDRN, one daily 5.625 mg/3 mL ampoule for 90 days with a three-month follow-up). Primary efficacy endpoints: Localized Scleroderma Cutaneous Assessment Tool mLoSSI and mLoSDI subscores for disease activity and damage in eighteen areas; Physicians Global Assessment for Activity (PGA-A) and Damage (PGA-D) VAS scores; skin echography. Secondary efficacy endpoints: mLoSSI, mLoSDI, PGA-A, PGA-D, and morphea areas (photographs) over time; Dermatology Life Quality Index (DLQI); skin biopsy scores and induration over time. Twenty-five patients enrolled; 20 completed the follow-up period. Highly significant improvements at the end of the 3-month treatment period: mLoSSI–73.7%, mLoSDI–43.9%, PGA-A–60.4%, PGA-D–40.3%, with further improvements at follow-up visit for all disease activity and damage indexes. Overall, the outcomes suggest that a daily PDRN ampoule intramuscularly for 90 days reduces disease activity and damage rapidly and significantly in quiescent, modestly inflammatory morphea with few currently therapeutic options. The COVID-19 pandemic and lockdowns caused difficulties in enrollment, and some patients were lost to follow-up. Due to low final enrollment, the study outcomes may have only an exploratory value, yet they appear impressive. The anti-dystrophic potential of the PDRN A2A adenosine agonist deserves further in-depth exploration.
Identifying the Aetiology of Acute Liver Failure Is Crucial to Impact Positively on Outcome
Authors: Di Giorgio Angelo , Gamba Silvia , Sansotta Naire , Nicastro Emanuele , Colledan Michele , D’Antiga Lorenzo
Published: April 2023

Management of children with acute liver failure is challenging. In this retrospective study, paediatric patients diagnosed with ALF at our centre, in the last 26 years, were divided into two groups (G1 = diagnosed from 1997 to 2009; G2 = from 2010 to 2022) and compared to see whether they differed with regard to aetiologies, need for liver transplantation (LT), and outcome. A total of 90 children (median age 4.6 years, range 1.2–10.4; M/F = 43/47) were diagnosed with ALF, by autoimmune hepatitis (AIH) in 16 (18%), paracetamol overdose in 10 (11%), Wilson disease in 8 (9%), and other causes in 19 (21%); 37 (41%) had indeterminate ALF (ID-ALF). Comparing the two periods, the clinical features, aetiologies, and median peak values of INR [3.8 (2.9–4.8) in G1 vs. 3.2 (2.4–4.8) in G2] were similar (p > 0.05). The percentage of ID-ALF tended to be higher in G1 compared to G2 (50% vs. 32% in G2, p = 0.09). The overall percentage of patients diagnosed with Wilson disease, inborn errors of metabolism, neonatal hemochromatosis or viral infection was higher in G2 (34% vs. 13% in G1, p = 0.02). A total of 21/90 patients (23%; 5 with indeterminate ALF) were treated with steroids; 12 (14%) required extracorporeal liver support treatment. The need for LT was significantly higher in G1 compared to G2 (56% vs. 34%; p = 0.032). Among 37 children with ID-ALF, 6 (16%) developed aplastic anaemia (all in G2, p < 0.001). The survival rate at last follow up was of 94%. On a KM curve, the transplant-free survival was lower in G1 compared to G2. In conclusion, we report a lower need for LT in children diagnosed with PALF during the most recent period compared to the first era. This suggests improvements over time in the diagnosis and management of children with PALF.
Statistical Medium Optimization for the Production of Anti-Methicillin-Resistant Staphylococcus aureus Metabolites from a Coal-Mining-Soil-Derived Streptomyces rochei CMB47
Authors: Djinni Ibtissem , Djoudi Warda , Boumezoued Chahinaz , Barchiche Halima , Souagui Samiha , Kecha Mouloud , Mancini Ines
Published: April 2023

The development of novel antibacterial drugs needs urgent action due to the global emergence of antibiotic resistance. In this challenge, actinobacterial strains from arid ecosystems are proving to be promising sources of new bioactive metabolites. The identified Streptomyces rochei strain CMB47, isolated from coal mine Saharan soil, provided an ethyl acetate extract which tested against a series of pathogens. It displayed a minimum inhibitory concentration of <0.439 µg/mL against MRSA. A statistical experimental design using a response surface methodology (RSM) based on the second-order rotatable central composite design (RCCD) was planned to develop an efficient fermentation process able to improve the bioactive metabolite production. The optimal conditions were determined for starch and NaNO3 concentrations, incubation time and the initial pH value, reaching the inhibition zone diameter of 20 mm, close to the experimental value, after validation of the model. A bioassay-guided fractionation of the crude extract provided the most active fractions, which were analyzed by HPLC equipped with a photodiode array detector and coupled online with an electrospray mass spectrometer (HPLC-DAD/ESI-MS), obtaining preliminary indications on the molecular structures of the metabolites. These results support the potential interest in further investigations into the purification and full characterization of the metabolites responsible for the biological activity observed so far.
Non-Conventional Sucrose-Based Substrates: Development of Non-Dairy Kefir Beverages with Probiotic Potential
Authors: Tavares Pedro Paulo Lordelo Guimarães , Mamona Clariane Teixeira Pessoa , Nascimento Renata Quartieri , dos Anjos Emanuele Araújo , de Souza Carolina Oliveira , Almeida Rogéria Comastri de Castro , Mamede Maria Eugênia de Oliveira , Magalhães-Guedes Karina Teixeira
Published: April 2023

There is a scarcity of studies evaluating the influence of different commonly marketed sugars in water kefir beverage production. Therefore, this study aimed to evaluate the fermentation of water kefir grains in different sugary solutions: brown, demerara, refined, coconut, and cane molasses. A total of 10% of each type of sugar was dissolved in sterile water to which 10% of kefir grains were then added and fermented for 48 h at room temperature. Analyses of pH/acidity, soluble solids, lactic/acetic acids, and lactic acid bacteria and yeast counts were performed, in addition to grain weighing at 0 h, 24 h, and 48 h. The microbial biodiversity was measured using PCR-DGGE and DNA sequencing at the species level. A sensory acceptance test was performed on all beverages. Lactobacillus, Lacticaseibacillus, Lentilactobacillus Lactococcus, Leuconostoc, Acetobacter, Saccharomyces, Kluyveromyces, Lachancea, and Kazachstania were present in the kefir grains and the beverages. Molasses showed a more intense fermentation, with greater production of organic acids and higher lactic/acetic acid bacteria and yeast counts (7.46 and 7.49 log CFU/mL, respectively). Refined sugar fermentation had a lower microbial yield of lactic/acetic acid bacteria (6.87 log CFU/mL). Smith’s salience index indicates that the brown-sugar kefir beverage was better accepted among the tasters. The results indicate that the use of alternative sources of sugar to produce water kefir beverages is satisfactory. This opens up new perspectives for the application of kefir microorganisms in the development of beverages with probiotic and functional properties.
Periodontal Healing with Fixed Restorations Using the Biologically Oriented Preparation Technique Combined with a Full Digital Workflow: A Clinical Case Report
Authors: Rinaldi Tommaso , Santamaría-Laorden Andrea , Orejas Pérez Jaime , Godoy Ruíz Laura , Serrano Granger Carlos , Gómez Cogolludo Pablo
Published: April 2023

(1) Gingival inflammation is an ongoing challenge in tooth-supported fixed restorations, especially when the prosthetic margin does not consider the supracrestal tissues of patients. This case report aimed to present the case of a patient who was periodontally compromised due to a previous invasion of the supracrestal tissue attachment with fixed restorations and evaluate the healing response of periodontal tissues to a vertical edgeless preparation technique: bleeding upon probing (BOP), periodontal probing depth (PPD) and clinical attachment level (CAL). (2) After tooth preparation, the new restorations were adapted, this time without invading the supracrestal space of the patient, and CAD/CAM monolithic zirconia crowns were fabricated. (3) Optimal maturation of the soft tissue was observed, achieving correction of the marginal contour of periodontal tissues and improvement of periodontal indexes. (4) It can be concluded that the BOPT technique combined with a full digital workflow is a valid option for the correction and remodeling of gingival architecture.
The Complexity of Burnout Experiences among Care Aides: A Person-Oriented Approach to Burnout Patterns
Authors: Duan Yinfei , Song Yuting , Thorne Trina E. , Iaconi Alba , Norton Peter G. , Estabrooks Carole A.
Published: April 2023

Care aides working in nursing homes experience burnout attributed to various workplace stressors. Burnout dimensions (exhaustion, cynicism, and reduced professional efficacy) interact to form distinct burnout patterns. Using a person-oriented approach, we aimed to identify burnout patterns among care aides and to examine their association with individual and job-related factors. This was a cross-sectional, secondary analysis of the Translating Research in Elder Care 2019–2020 survey data collected from 3765 care aides working in Canadian nursing homes. We used Maslach Burnout Inventory to assess burnout and performed latent profile analysis to identify burnout patterns, then examined their associations with other factors. We identified an engaged pattern (43.2% of the care aide sample) with low exhaustion and cynicism and high professional efficacy; an overwhelmed but accomplished pattern (38.5%) with high levels of the three dimensions; two intermediate patterns—a tired and ineffective pattern (2.4%) and a tired but effective pattern (15.8%). The engaged group reported the most favorable scores on work environment, work-life experiences, and health, whereas the tired and ineffective group reported the least favorable scores. The findings suggest complex experiences of burnout among care aides and call for tailored interventions to distinct burnout patterns.
Breast Reconstruction with DIEP Flap: The Learning Curve at a Breast Reconstruction Center and a Single-Surgeon Study
Authors: Varnava Charalampos , Wiebringhaus Philipp , Hirsch Tobias , Dermietzel Alexander , Kueckelhaus Maximilian
Published: April 2023

Although microsurgical breast reconstruction represents a very interesting and rewarding field of plastic surgery, appropriate microsurgical training is not possible in every plastic surgery department. In this retrospective study, we present the learning curve of our plastic surgery department as a whole and of a single microsurgeon assessing breast reconstruction procedures with a deep inferior epigastric artery perforator (DIEP) flap between July 2018 and June 2021. The present study included 115 patients and 161 flaps. Cases were stratified into single DIEP/double DIEP groups and into early and late groups based on the flap order. Surgery times and postoperative complications were analyzed. Regarding the institution, the length of hospital stay was lower in the late group than in the early group (single 7.1 ± 1.8 vs. 6.3 ± 1.5 days, p = 0.019; double 8.5 ± 3.8 vs. 6.6 ± 1.4 days, p = 0.043). Apart from that, no statistically significant differences were found between the start and end of our study. In terms of the single surgeon, there was a significant improvement in the total surgery time (single 296.0 ± 78.7 vs. 227.5 ± 54.7 min, p = 0.018; double 448.0 ± 85.6 vs. 341.2 ± 43.1 min, p = 0.008), flap ischemia time (53.6 ± 15.1 vs. 40.9 ± 9.5 min, p = 0.007) and length of stay among the compared groups. There was no significant difference in flap loss rate or other complications between the early and late groups. Further performance of surgeries seemed to improve the surgeon’s skills as well as the overall experience of the medical institution.
Analysis of Tool Wear and Hole Delamination for Large-Diameter Drilling of CFRP Aircraft Fuselage Components: Identifying Performance Improvement Drivers and Optimization Opportunities
Authors: Fernández-Pérez Juan , Domínguez-Monferrer Carlos , Miguélez María Henar , Cantero José Luis
Published: April 2023

This study provides a comprehensive analysis of the one-shot drilling (CFRP) strategy for machining CFRP materials in the assembly of aircraft components, focusing on key factors such as tool wear, hole delamination, and the evolution of machining forces. The research uses adapted parts of the tail-cone structure of a commercial aircraft as workpieces and employs large-diameter cutting tools to perform drilling operations, with results that can be readily applied to the industry. The study selects cutting conditions by analyzing the effect of cutting parameters on tool life in drilled holes and accumulated cutting time, with the end-of-life criterion based on the extension of the wear suffered by the main cutting edge of the first step. The results show that all tested cutting conditions achieve a similar value of tool life expressed in terms of holes drilled, with differences smaller than 7%. However, one of the cutting conditions analyzed completes the same number of holes within 40% less time. Therefore, considering productivity criteria, it will be interesting to evaluate the use of high values for the cutting parameters. Overall, this research provides valuable insights for improving the efficiency and effectiveness of CFRP machining in aircraft manufacturing.
VGAE-AMF: A Novel Topology Reconstruction Algorithm for Invulnerability of Ocean Wireless Sensor Networks Based on Graph Neural Network
Authors: Zhang Ying , Zhang Qi , Zhang Yu , Zhu Zhiyuan
Published: April 2023

Ocean wireless sensor networks (OWSNs) play an important role in marine environment monitoring, underwater target tracking, and marine defense. OWSNs not only monitor the surface information in real time but also act as an important relay layer for underwater sensor networks to establish data communication between underwater sensors and ship-based base stations, land-based base stations, and satellites. The destructive resistance of OWSNs is closely related to the marine environment where they are located. Affected by the dynamics of seawater, the location of nodes is extremely easy to shift, resulting in the deterioration of the connectivity of the OWSNs and the instability of the network topology. In this paper, a novel topology optimization model of OWSNs based on the idea of link prediction by cascading variational graph auto-encoders and adaptive multilayer filter (VGAE-AMF) was proposed, which attenuates the extent of damage after the network is attacked, extracts the global features of OWSNs by graph convolutional network (GCN) to obtain the graph embedding vector of the network so as to decode and generate a new topology, and finally, an adaptive multilayer filter (AMF) is used to achieve topology control at the node level. Simulation experiment results show that the robustness index of the optimized network is improved by 39.65% and has good invulnerability to both random and deliberate attacks.
The Effects of Cross-Language Differences on Bilingual Production and/or Perception of Sentence-Level Intonation
Authors: Colantoni Laura , Mennen Ineke
Published: April 2023

We put together this Special Issue with the goal of collecting state-of-the art articles on the intonational patterns of different types of bilinguals, with a particular focus on understudied language pairings, and we believe that we have succeeded [...]
Dirty Windows and Troublesome Things: The Problem of Object-Orientation in Alain Robbe-Grillet’s La Jalousie
Authors: Zuliani Andy
Published: April 2023

This article investigates the representation of objects in La Jalousie (1957), a novel in the nouveau roman tradition written by French novelist Alain Robbe-Grillet. If the ‘new novel’ sought to render the material world with objective clarity, and positioned itself against traditional fiction, with its reliance on metaphor, allegory, and other ‘projections,’ this article argues that such an aesthetic program is undercut by its own assumptions about the power of description and the primacy of the visual. In an analysis which hybridizes three separate strands of criticism—object-oriented ontology, Heideggerian phenomenology, and the models of ‘resonation’ proposed by Brian Massumi—I will argue that such a treatment of objects, with its exclusive reliance on visual description, measurement, and enumeration, ends up depriving objects of the vitality and dynamism that would justify such a fictional project in the first place. However, traces of this dynamism do survive the flattening sweep of Robbe-Grillet’s narration, and indeed offer from the cracks and fissures of the novel’s otherwise smoothly controlled style the possibility of an alternate ‘object-orientation’—one, I will argue, which suspends its cool optical detachment to allow, however briefly, the eruption of a messy, entangling register of touch.
Unsteady Hydromagnetic Flow over an Inclined Rotating Disk through Neural Networking Approach
Authors: Ali Ishtiaq , Gul Taza , Khan Arshad
Published: April 2023

The goal of this research is to examine how a magnetic field affects the unsteady flow of a hybrid nanofluid over a spinning disk that is inclined and stretched while the flow is surrounded by a non-Darcy porous medium. Furthermore, for heat transmission mechanisms, Joule heating and viscous dissipation are considered. The current article is made more realistic by imposing thermal radiation to enhance the heat transmission system under the effects of convection. Moreover, thermal and velocity slip conditions have also been incorporated into the current study. The equations that administer the flow problem along with constraints at the boundaries are converted to dimension-free form by employing a set of appropriate similarity transformations, which are then solved by the numerical technique Runge-Kutta method of order four (RK-4). The new and advanced trend for the convergence of the obtained results is validated through a neural networking approach. The temperature of hybrid nanofluid is augmented by an increase in the porosity parameter, the unsteadiness factor, the Eckert number, the magnetic field, and the Forchheimmer number, while for the values of the radiation factor, the thermal heat is decreasing near the disk and increasing away from the disk. The precision of the obtained results has been ensured by comparing them with established results, with good agreement among these results.
Humeral Head Three-Part Posterior Fracture-Dislocation Reduced through a Posterior Approach and Fixed with Blocked Threaded Wires: A Consecutive Case Series
Authors: Gumina Stefano , Candela Vittorio
Published: April 2023

Background and Objectives: Posterior fracture dislocations are rare. There is currently no uniformity regarding treatment. Therefore, outcomes are difficult to compare. We evaluated clinical and radiological outcomes of patients with humeral head posterior fracture dislocation treated with an open posterior reduction and then fixed with a biomechanically validated configuration of blocked threaded wires. Materials and Methods: 11 consecutive patients with humeral head three-part posterior fracture dislocation were treated by reduction through a posterior approach and fixed with blocked threaded wires. All patients were clinically and radiographically evaluated after a mean follow-up of 50 months. Results: The mean irCS was 86.1% (range: 70.5–95.3%). No significant difference was found between irCS at 6 and 12 months postoperatively and the final follow-up. Six patients noted their pain intensity as 0/10, three as 1/10, and two as 2/10. The postoperative reduction was considered as excellent in eight patients (Bahr’s criteria) and good in the remaining three; at the final follow-up, reduction was excellent and good in seven and four patients, respectively. The mean neck-shaft angles at FU 0 and at the final FU were 137° and 132°, respectively. No signs of avascular necrosis, non-union, and arthritis progression were seen. No recurrence of dislocation or posterior instability symptoms were reported. Conclusions: We believe that our very satisfactory results stem from: (1) the manual reduction of the dislocation through a vertical posterior surgical approach, which does not produce further osteocartilaginous damage of the humeral head; (2) no multiple perforations of the humeral head are performed; (3) the threaded wires have a smaller diameter than the screws, therefore they preserve the bone tissue of the humeral head; (4) deperiostization or further detachment of soft tissues are not expected; (5) the adopted and validated system is stable and limits translation, torsion, and the collapse of the humeral head.
Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra
Authors: Alemneh Sendeku Takele , Babor Majharulislam , Zettel Viktoria , von Wrochem Almut , Hitzmann Bernd
Published: April 2023

The demand for probiotic bacteria-fermented food products is increasing; however, the monitoring of the fermentation process is still challenging when using conventional approaches. A classical approach requires a large amount of offline data to calibrate a chemometric model using fluorescence spectra. Fluorescence spectra provide a wide range of online information during the process of cultivation, but they require a large amount of offline data (which involves laborious work) for the calibration procedure when using a classical approach. In this study, an alternative model-based calibration approach was used to predict biomass (the growth of Lactiplantibacillus plantarum A6 (LPA6) and Lacticaseibacillus rhamnosus GG (LCGG)), glucose, and lactic acid during the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. A classical approach was also applied and compared to the model-based calibration approach. In the model-based calibration approach, two-dimensional (2D) fluorescence spectra and offline substituted simulated data were used to generate a chemometric model. The optimum microbial specific growth rate and chemometric model parameters were obtained simultaneously using a particle swarm optimization algorithm. The prediction errors for biomass, glucose, and lactic acid concentrations were measured between 6.1 and 10.5%; the minimum error value was related to the prediction of biomass and the maximum one was related to the prediction of glucose using the model-based calibration approach. The model-based calibration approach and the classical approach showed similar results. In conclusion, the findings showed that a model-based calibration approach could be used to monitor the process state variables (i.e., biomass, glucose, and lactic acid) online in the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. However, glucose prediction showed a high error value.
Evaluating Antimicrobial Resistance Trends in Commensal Escherichia coli Isolated from Cecal Samples of Swine at Slaughter in the United States, 2013–2019
Authors: Sodagari Hamid Reza , Varga Csaba
Published: April 2023

The emergence of antimicrobial resistance (AMR) in commensal and pathogenic enteric bacteria of swine is a public health threat. This study evaluated publicly available AMR surveillance data collected by the National Antimicrobial Resistance Monitoring System (NARMS) by assessing AMR patterns and temporal trends in commensal E. coli isolated from cecal samples of swine at slaughter across the United States. We applied the Mann-Kendall test (MKT) and a linear regression trend line to detect significant trends in the proportion of resistant isolates to individual antimicrobials over the study period. A Poisson regression model assessed differences among years in the number of antimicrobials to which an E. coli isolate was resistant. Among the 3237 E. coli isolates, a very high prevalence of resistance for tetracycline (67.62%), and high resistance for streptomycin (24.13%), and ampicillin (21.10%) were identified. The MKT and the linear trend line showed a significantly increasing temporal trend for amoxicillin-clavulanic acid, ampicillin, azithromycin, cefoxitin, ceftriaxone, and trimethoprim-sulfamethoxazole. Compared to 2013 the number of antimicrobials to which an E. coli isolate was resistant was significantly higher in the years 2017, 2018, and 2019. The increasing temporal trend of resistance to important antimicrobials for human medicine (e.g., third-generation cephalosporins) and the increase in multidrug resistance in the later years of the study are concerning and should be followed up by studies to identify sources and risk factors for the selection of AMR.
Regulation of Staphylococcal Enterotoxin-Induced Inflammation in Spleen Cells from Diabetic Mice by Polyphenols
Authors: Shimamura Yuko , Noaki Rina , Oura Yukino , Ichikawa Kenya , Kan Toshiyuki , Masuda Shuichi
Published: April 2023

Patients with diabetes are known to be more susceptible to infections following the establishment of Staphylococcus aureus in their nasal passages and on their skin. The present study evaluated the effects of staphylococcal enterotoxin A (SEA) on the immune responses of spleen cells derived from diabetic mice, and examined the effects of polyphenols, catechins, and nobiletin on inflammation-related gene expression associated with the immune response. (−)-Epigallocatechin gallate (EGCG), possessing hydroxyl groups, interacted with SEA, whereas nobiletin, possessing methyl groups, did not interact with SEA. The exposure of spleen cells derived from diabetic mice to SEA enhanced the expression of interferon gamma, suppressor of cytokine signaling 1, signal transducer and activator of transcription 3, interferon-induced transmembrane protein 3, Janus kinase 2, and interferon regulatory factor 3, suggesting that SEA sensitivity is variable in the development of diabetes. Both EGCG and nobiletin changed the expression of genes related to SEA-induced inflammation in spleen cells, suggesting that they inhibit inflammation through different mechanisms. These results may lead to a better understanding of the SEA-induced inflammatory response during diabetogenesis, and the establishment of methods to control these effects with polyphenols.
Characterization of the Oral Microbiome in Wearers of Fixed and Removable Implant or Non-Implant-Supported Prostheses in Healthy and Pathological Oral Conditions: A Narrative Review
Authors: D’Ambrosio Francesco , Santella Biagio , Di Palo Maria Pia , Giordano Francesco , Lo Giudice Roberto
Published: April 2023

Oral commensal microorganisms perform very important functions such as contributing to the health of the host. However, the oral microbiota also plays an important role in the pathogenesis and development of various oral and systemic diseases. The oral microbiome may be characterized by a higher prevalence of some microorganisms than others in subjects with removable or fixed prostheses, depending on oral health conditions, the prosthetic materials used, and any pathological conditions brought about by inadequate prosthetic manufacturing or poor oral hygiene. Both biotic and abiotic surfaces of removable and fixed prostheses can be easily colonized by bacteria, fungi, and viruses, which can become potential pathogens. The oral hygiene of denture wearers is often inadequate, and this can promote oral dysbiosis and the switch of microorganisms from commensal to pathogens. In light of what emerged from this review, fixed and removable dental prostheses on teeth and on implants are subject to bacterial colonization and can contribute to the formation of bacterial plaque. It is of fundamental importance to carry out the daily hygiene procedures of prosthetic products, to design the prosthesis to facilitate the patient’s home oral hygiene practices, and to use products against plaque accumulation or capable of reducing oral dysbiosis to improve patients’ home oral practices. Therefore, this review primarily aimed to analyze the oral microbiome composition in fixed and removable implant or non-implant-supported prostheses wearers in healthy and pathological oral conditions. Secondly, this review aims to point out related periodontal self-care recommendations for oral dysbiosis prevention and periodontal health maintenance in fixed and removable implant or non-implant-supported prostheses wearers.