bluuebunny/crossref_metadata_2025 huggingface dataset:
Pine Bark as a Potential Source of Condensed Tannin: Analysis through Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX)
Authors: Feria-Reyes Rossy , Ramírez-Cruz Sergio Obed , Ruiz-Aquino Faustino , Robledo-Taboada Luis Humberto , Sánchez-Medina Marco Antonio , Mijangos-Ricárdez Oscar Francisco , Gabriel-Parra Rosalío , Suárez-Mota Mario Ernesto , Puc-Kauil Ramiro , Porcallo-Vargas Jhazeel
Published: July 2023

This study aimed to evaluate the tannin content in the bark of five pine species from a forest harvesting area of the Ixtlán de Juárez community, Oaxaca, México. The species studied were Pinus patula, Pinus ayacahuite, Pinus rudis, Pinus douglasiana, Pinus pseudostrobus. The bark samples were subjected to a drying, grinding, and sieving process. These compounds were extracted using two methods: by constant reflux with ethanol for three hours or in a water bath with distilled water for two hours. The percentage of total extract, Stiasny number, and condensed tannins were quantified. The quantitative number of condensed tannins present in the bark for each of the five species studied ranged from 0.65% to 5.14% for the ethanolic extracts and 0.14% to 1.46% for the aqueous extracts. Analysis by Fourier transform infrared spectroscopy (FTIR) identified polyphenolic compounds and functional groups characteristic of tannins. Analysis by scanning electron microscopy (SEM) and X-ray dispersive spectroscopy (EDX) provided the elemental analysis and chemical composition of the tannin extracts, respectively, finding trace elements of silver, cadmium, phosphorus, iodine, and sulfur, which are minerals present in the soil, meaning that through processes of micronutrient absorption, these can interact with the tannins or polyphenols in the barks of the tree species being studied. The results indicate that the bark of P. patula and P. ayacahuite have a higher concentration of condensed tannins, 5.14% and 4.71%, respectively, in the ethanol extraction, and may be susceptible to exploitation due to the amount of bark generated in forestry activities.
Ecological Adaptation of Two Dominant Conifer Species to Extreme Climate in the Tianshan Mountains
Authors: Wu Xuan , Jiao Liang , Liu Xiaoping , Xue Ruhong , Qi Changliang , Du Dashi
Published: July 2023

With global warming, the frequency, intensity, and period of extreme climates in more areas will probably increase in the twenty first century. However, the impact of climate extremes on forest vulnerability and the mechanisms by which forests adapt to climate extremes are not clear. The eastern Tianshan Mountains, set within the arid and dry region of Central Asia, is very sensitive to climate change. In this paper, the response of Picea schrenkiana and Larix sibirica to climate fluctuations and their stability were analyzed by Pearson’s correlation based on the observation of interannual change rates of climate indexes in different periods. Additionally, their ecological adaptability to future climate change was explored by regression analysis of climate factors and a selection of master control factors using the Lasso model. We found that the climate has undergone significant changes, especially the temperature, from 1958 to 2012. Around 1985, various extreme climate indexes had obvious abrupt changes. The research results suggested that: (1) the responses of the two tree species to extreme climate changed significantly after the change in temperature; (2) Schrenk spruce was more sensitive than Siberian larch to extreme climate change; and (3) the resistance of Siberian larch was higher than that of Schrenk spruce when faced with climate disturbance events. These results indicate that extreme climate changes will significantly interfere with the trees radial growth. At the same time, scientific management and maintenance measures are taken for different extreme weather events and different tree species.
Evaluation of Soundscape Perception in Urban Forests Using Acoustic Indices: A Case Study in Beijing
Authors: Bian Qi , Zhang Chang , Wang Cheng , Yin Luqin , Han Wenjing , Zhang Shujing
Published: July 2023

Soundscape is an essential component of urban forest landscapes, acoustic indices can be effectively used to monitor biodiversity, but whether they can be used for soundscape perception assessments needs to be further explored. In this study, soundscape recordings were collected in Beijing Eastern Suburban Forest Park, and acoustic indices were used to explore the relationship between the acoustic environment and soundscape perception, as well as the possible effects of temporal changes. To achieve this, audio recordings collected in spring and summer were divided, and a total of 90 audio segments were extracted from three time periods—morning, afternoon, and evening—to calculate the acoustic index and complete a questionnaire survey. The urban forest soundscape was evaluated according to the eight perceptual attribute quality indicators of ISO 12913, and generalized linear models were constructed to quantify the relationships between the acoustic indices and perception. The results showed that the temporal variation of the soundscape influenced the subjective evaluation, with the highest overall evaluation relating to the morning soundscape. The combination of acoustic indices explained the soundscape pleasantness (R2 = 0.58) better than the soundscape eventfulness (R2 = 0.54), demonstrating the utility of these indices in soundscape assessment. Linking acoustic indices to human perception generates innovative ideas and theoretical support for soundscape enhancement, contributing to a more pleasant acoustic environment and maximizing the social value of urban forests.
Transcriptome Analysis Reveals the Hormone Signalling Coexpression Pathways Involved in Adventitious Root Formation in Populus
Authors: Zhang Qiqi , Shi Meirong , Tang Fang , Su Nan , Jin Feiyang , Pan Yining , Chu Liwei , Lu Mengzhu , Shu Wenbo , Li Jingcai
Published: July 2023

Adventitious roots (ARs) occur naturally in many species and are important for plants to absorb nutrients and water. AR formation can also be induced from explants of trees, whose clonal propagation is needed. AR formation is gridlock for many woody plant mass propagations. Plant hormones have been regarded as playing a key role in AR formation, and the molecular regulatory mechanisms need to be elucidated. In this study, RNA-Seq was performed to reveal the molecular mechanisms in the different periods of AR formation from hybrid poplar clone 84K (Populus alba × P. glandulosa) and AUXIN SIGNALING F-BOX (PagFBL1-OE). To understand the importance of differentially expressed genes (DEGs), we found that many genes involved in signal transduction mechanisms were induced at 12, 24 and 48 h in 84K and PagFBL1-OE cells by NOG classification. We also found that many DEGs were enriched in hormone signal transduction only for the first 12 h in 84K and PagFBL1-OE by KEGG pathway enrichment. Notably, more DEGs appeared in indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene (ETH), jasmonic acid (JA), brassinolide (BR), cytokinin (CTK) and gibberellin (GA) signal transduction for the first 12 h in PagFBL1-OE than in 84K. Moreover, ARF (Pop_G01G075686), IAA14 (Pop_A10G047257), SAURs (Pop_A03G019756, Pop_A12G067965, Pop_G03G055849 and Pop_G12G008821), JAR1s (Pop_A14G000375 and Pop_G14G044264), CTR1 (Pop_A17G052594 and Pop_G09G030293), CRE1s (Pop_G07G086605 and Pop_G07G086618), GID1 (Pop_A04G026477), BKI1 (Pop_A02G066155), PYR/PYLs (Pop_A03G050217 and Pop_G01G089222), and TGAs (Pop_A04G059310, Pop_G04G060065 and Pop_G05G008153) were only specifically expressed in PagFBL1-OE and could play an important role in AR formation, especially in the first 12 h under plant hormone signal transduction. These results show that the complex biological process of AR formation is primarily influenced by the hormone signalling pathway in Populus. This study reveals the initial regulation of AR formation in woody plant cuttings and thus contributes to further elucidating the molecular mechanism by which hormones interact.
The Most Suitable Calcium Concentration for Growth Varies among Different Tree Species—Taking Pinus tabuliformis, Pinus sylvestris var. mongolica, Populus, and Morus alba as Examples
Authors: Li Hui , Zhao Yaoyao , Weng Xiaohang , Zhou Yongbin , Zhang Songzhu , Liu Liying , Pei Jiubo
Published: July 2023

Ca2+ is a crucial second messenger in plant cells that plays a vital role in various physiological and biochemical processes, including plant growth and development, photosynthesis, and enzyme regulation. Exogenous calcium concentrations can have different effects on plant growth. The purpose of this study was to determine the appropriate calcium concentration for the growth of four tree seedlings, namely Pinus tabuliformis, Pinus sylvestris var. mongolica, Populus, and Morus alba seedlings, and whether this optimal calcium concentration varies among different tree species. This study utilized five calcium concentration gradients (0, 100, 200, 400, 800 mg·kg−1) for each species with three repeated treatments. To determine each species’ calcium concentration needs, several growth indices, photosynthetic metrics, chlorophyll fluorescence parameters, water usage efficiency, and antioxidant enzyme activities were analyzed. The results showed that exogenous calcium significantly affected the growth and development of each seedling. The growth, biomass, photosynthetic parameters, photosynthetic products, photosynthetic pigments, water use efficiency, and antioxidant enzyme activity all increased initially and then decreased with the increasing calcium concentration. The exogenous calcium supply increased the concentration of calcium in the leaves. Thus, there was an optimal calcium concentration for plant growth, and a high or low calcium concentration was not conducive to plant growth. Furthermore, this study found that the optimum calcium concentration of different tree species was different. The optimal calcium concentration for Pinus tabuliformis, Pinus sylvestris var. mongolica, Populus, and Morus alba seedlings was 100, 100, 200, and 400 mg·kg−1, respectively, with broadleaf seedlings requiring higher calcium concentrations than coniferous seedlings.
Spatial and Temporal Patterns of Non-Structural Carbohydrates in Faxon Fir (Abies fargesii var. faxoniana), Subalpine Mountains of Southwest China
Authors: Pan Shao’an , Anees Shoaib Ahmad , Li Xuhua , Yang Xinrui , Duan Xiangguang , Li Zhigang
Published: July 2023

Non-structural carbohydrates’ (NSCs) allocation pattern is closely related to environmental factors, plant metabolism, and xylem function. At the same time, we know little about whole-tree NSC allocation patterns in different seasons, especially the high-elevation species which suffer from environmental stress. We examined the concentration of NSCs in various parts of Faxon fir trees (needles, branches, trunks, and roots) at five elevations (2800 m, 3000 m, 3200 m, 3400 m, and 3600 m) over four months (July 2019, October 2019, January 2020, and April 2020). The goal was to understand how NSC allocation patterns vary by location and time in high-elevation species and what factors contribute to these variations. The results showed that the needles had the highest concentration, followed by roots, branches, and trunks. The NSC concentration was highest in January 2020 and lowest in July 2019. The total non-structural carbohydrates (TNSCs) and soluble sugar concentrations of roots and needles were substantially higher in the cold (non-growing season) than in the warm (growing season) season. At different elevations, the soluble sugar concentrations in the needles and trunks remained the highest and lowest, respectively. Branches and roots’ soluble sugar concentrations alternated and varied with the seasons at all elevations. Many factors, such as climate, morphological traits, and carbon content, affected the spatial and temporal patterns of non-structural carbohydrates, with temperature, plant moisture conditions, and carbon content being the main driving factors. Various factors’ interaction mainly influenced NSCs’ spatial and temporal patterns. Non-structural carbohydrates significantly improve the resistance of Faxon fir trees’ terminal organs in adverse environments.
Shiro-like Structure Formation of Chinese Tricholoma matsutake Strain YN1 in Pinus armandii and Pinus elliottii Seedlings
Authors: Chen Xin , Mou Chunye , Zhang Qianqian , Bian Yinbing , Kang Heng
Published: July 2023

Tricholoma matsutake is one of the edible ectomycorrhizal fungi with great economic and ecological values. Artificially mycorrhized seedlings inoculated with T. matsutake strains from Finland and Japan have been widely reported. However, few reports on the morphological characteristics of mycorrhizae synthesized from the Chinese T. matsutake strain are available to date. Here, we find that the T. matsutake strain YN1 from Yunnan province, China, can form well-developed ectomycorrhizae in symbiosis with both exotic (Pinus elliottii) and Chinese native (Pinus armandii) species. The symbiotic structures of ectomycorrhizae included obvious mantle sheaths and Hartig nets visible under a microscope. The ectomycorrhizae appeared earlier in a larger quantity in P. elliottii than in P. armandii in the culture-plate symbiotic system. The ectomycorrhizae obviously promoted the growth of potted P. armandii and P. elliottii, accompanied by the formation of shiro-like structures. Our results provide references for the effective management of ectomycorrhizae synthesis in both P. armandii and P. elliottii seedlings to form shiro-like structures, and also provide a new perspective for the afforestation and mushroom cultivation research on T. matsutake.
The Effect of Long-Term Passage on Porcine SMCs’ Function and the Improvement of TGF-β1 on Porcine SMCs’ Secretory Function in Late Passage
Authors: Zheng Yan-Yan , Hu Ze-Nan , Liu Zheng , Jiang Yi-Chen , Guo Ren-Peng , Ding Shi-Jie , Zhou Guang-Hong
Published: July 2023

Cultured meat is one of the meat substitutes produced through tissue engineering and other technologies. Large-scale cell culture is the key for cultured meat products to enter the market. Therefore, this study is aimed to explore the effect of long-term passage in vitro on smooth muscle cells (SMCs) and the effect of transforming growth factor-β1 (TGF-β1) on SMCs in the late passage. Multiple passages lead to the decline of the proliferation rate of SMCs in the proliferation stage and the differentiation ability in the differentiation stage. Transcriptome results showed that the ECM pathway and aging-related signaling pathways were significantly up-regulated in the late passage period. TGF-β1 did not promote SMCs of late passage proliferation at the proliferation stage but promoted the gene and protein expression of collagen as the main protein of the extracellular matrix proteins at the differentiation stage. In addition, proteomic analysis revealed that TGF-β1 promoted the expression of cell adhesion molecules which activate the Hippo signaling pathway and the HIF-1 signaling pathway and further promoted the production of collagen-containing extracellular matrix proteins. This could provide ideas for large-scale production of cultured meat products using SMCs.
Comparison of Drying Techniques for Extraction of Bioactive Compounds from Olive-Tree Materials
Authors: Castillo-Luna Ana , Miho Hristofor , Ledesma-Escobar Carlos A. , Priego-Capote Feliciano
Published: July 2023

Olive tree vegetal materials are considered a powerful source for the isolation of bioactive compounds—mainly phenols and triterpenic acids. However, the high humidity content of them reduces their preservation and extractability to a liquid solvent. Accordingly, a drying step is crucial to homogenize the material and to obtain an efficient extraction. We studied the influence of the drying process on the extraction efficiency of bioactive compounds from olive vegetal material. For this purpose, we evaluated the effects of four drying processes on the solid–liquid extraction of bioactive compounds from two by-products, olive leaves and pomace, and olive fruits harvested from two cultivars, Alfafara and Koroneiki. Infrared-assisted drying (IAD) was the most suited approach to obtain extracts enriched in oleuropein from leaves (28.5 and 22.2% dry weight in Alfafara and Koroneiki, respectively). In the case of pomace, lyophilization and microwave-assisted drying led to extracts concentrated in oleacein and oleuropein aglycone, whereas IAD and oven-drying led to extracts with enhanced contents of hydroxytyrosol glucoside and hydroxytyrosol, respectively. The drying process considerably affected the chemical composition of extracts obtained from fruits. Changes in the composition of the extracts were explained essentially by the drying process conditions using auxiliary energies, temperature, and time, which promoted chemical alterations and increased the extractability of the compounds. Therefore, the drying protocol should be selected depending on the phenolic content and initial raw material.
A Study of the Gelatin Low-Temperature Deposition Manufacturing Forming Process Based on Fluid Numerical Simulation
Authors: Tong Qiang , Zhao Wentao , Guo Tairong , Wang Dequan , Dong Xiuping
Published: July 2023

Low-temperature deposition manufacturing has attracted much attention as a novel printing method, bringing new opportunities and directions for the development of biological 3D printing and complex-shaped food printing. In this article, we investigated the rheological and printing properties of gelatin solution and conducted numerical simulation and experimental research on the low-temperature extrusion process of gelatin solution. The velocity, local shear rate, viscosity, and pressure distribution of the material in the extrusion process were calculated using Comsol software. The effects of the initial temperature, inlet velocity, and print head diameter of the material on the flow field distribution and printing quality were explored. The results show that: (1) the fluidity and mechanical properties of gelatin solution vary with its concentration; (2) the initial temperature of material, inlet velocity, and print head diameter all have varying degrees of influence on the distribution of the flow field; (3) the concentration change of the material mainly affects the pressure distribution in the flow channel; (4) the greater the inlet velocity, the greater the velocity and shear rate in the flow field and the higher the temperature of the material in the outlet section; and (5) the higher the initial temperature of the gel, the lower the viscosity in the flow field. This article is of great reference value for the low-temperature 3D printing of colloidal materials that are difficult to form at room temperature.
Simplified Formula for Estimating Nasal Dimensions for 3-Dimensional Facial Reconstruction among Japanese Adults
Authors: Matsuda Haruto , Kawashima Tomokazu , Sato Fumi
Published: July 2023

The eyelids, external nose, and lips play an important role in individual identification and facial recognition; however, they are excluded from tissue marker points, and are reconstructed based on generic methods for 3D facial reconstruction or facial approximation. Therefore, this study focused on nasal dimensions and evaluated whether Krogman’s widely used formula for estimating the dimensions of an external nose, regardless of sex, race, and body physique, can be applied to Japanese adults. A total of 146 postmortem CT images of Japanese adult cadavers (64 males and 82 females, aged 58–105 years old) were retrospectively analyzed. The total nasal projection (TNP) among Japanese adults was estimated using the formula, TNP = 1.9 × the anterior nasal spine projection (ANSP) + the mid-philtrum depth (MPD), which differed significantly from the coefficient (3.0) in the conventional formula, regardless of sex, race, and body physique, and therefore needed modification for Asians. Although there was no positive relationship between the total nasal width (TNW) and the maximum width of the anterior nasal aperture (ANAW), the TNW could be estimated by adding soft tissue that varies by sex and body physique to both sides of the nearly constant ANAW. Therefore, we determined a simple and practical formula to estimate nasal dimensions among Japanese adults for conventional 3D facial reconstruction and manual 3D facial sculpture.
Maturity-Associated Polygenic Profiles of under 12–16-Compared to under 17–23-Year-Old Male English Academy Football Players
Authors: McAuley Alexander B. T. , Varley Ian , Herbert Adam J. , Suraci Bruce , Baker Joseph , Johnston Kathryn , Kelly Adam L.
Published: July 2023

The purpose of this study was to examine polygenic profiles previously associated with maturity timing in male academy football players across different age phases. Thus, 159 male football players from four English academies (U12–16, n = 86, aged 13.58 ± 1.58 years; U17–23, n = 73, aged 18.07 ± 1.69 years) and 240 male European controls were examined. Polygenic profiles comprised 39 single nucleotide polymorphisms and were analysed using unweighted and weighted total genotype scores (TGSs; TWGSs). There were significant differences in polygenic profiles between groups, whereby U17–23 players had more genetic variants associated with later maturity compared to U12–16 players (TGS, p = 0.010; TWGS, p = 0.024) and controls (TGS, p = 0.038; TWGS, p = 0.020). More specifically, U17–23 players had over two-times the odds of possessing >36 later-maturing alleles than <30 compared to U12–16 players (odds ratio (OR) = 2.84) and controls (OR = 2.08). These results suggest there was a greater proportion of relatively later-maturing players as maturation plateaus towards adulthood, which may be explained by the ‘underdog hypothesis’. This study provides the first known molecular evidence that supports the notion that a maturity selection bias exists within male academy football.
Optimised Agrobacterium-Mediated Transformation and Application of Developmental Regulators Improve Regeneration Efficiency in Melons
Authors: Wan Lili , Wang Zhuanrong , Zhang Xuejun , Zeng Hongxia , Ren Jian , Zhang Na , Sun Yuhong , Mi Tang
Published: July 2023

Melon (Cucumis melo L.) is a protected crop in China with high economic value. Agrobacterium-mediated genetic transformation is a powerful tool to improve agronomic traits and obtain elite germplasm. However, current transformation protocols in melons are inefficient and highly genotype-dependent. To improve transformation in melon, we tested different infiltration methods for Agrobacterium-mediated transformation. Among these methods, micro-brushing and sonication for 20 s, followed by vacuum infiltration at −1.0 kPa for 90 s, resulted in the strongest green fluorescent protein signal and increased the proportion of infected explants. We transformed melon with developmental regulatory genes AtGRF5, AtPLT5, AtBBM, AtWUS, AtWOX5, and AtWIND1 from Arabidopsis and estimated regeneration frequencies as the number of regenerating shoots/total number of inoculated explants in the selection medium. The overexpression of AtGRF5 and AtPLT5 in melon resulted in transformation efficiencies of 42.3% and 33% in ZHF and 45.6% and 32.9% in Z12, respectively, which were significantly higher than those of the control. AtGRF5 and AtPLT5 expression cassettes were added to CRISPR/Cas9 genome-editing vectors to obtain transgenic phytoene desaturase CmPDS knockout mutants. Using AtGRF5 or AtPLT5, multi-allelic mutations were observed at CmPDS target sites in recalcitrant melon genotypes. This strategy enables genotype-flexible transformation and promotes precise genome modification technologies in melons.
Ploidy in Vibrio natriegens: Very Dynamic and Rapidly Changing Copy Numbers of Both Chromosomes
Authors: Brück Patrik , Wasser Daniel , Soppa Jörg
Published: July 2023

Vibrio natriegens is the fastest-growing bacterium, with a doubling time of approximately 12–14 min. It has a high potential for basic research and biotechnological applications, e.g., it can be used for the cell-free production of (labeled) heterologous proteins, for synthetic biological applications, and for the production of various compounds. However, the ploidy level in V. natriegens remains unknown. At nine time points throughout the growth curve, we analyzed the numbers of origins and termini of both chromosomes with qPCR and the relative abundances of all genomic sites with marker frequency analyses. During the lag phase until early exponential growth, the origin copy number and origin/terminus ratio of chromosome 1 increased severalfold, but the increase was lower for chromosome 2. This increase was paralleled by an increase in cell volume. During the exponential phase, the origin/terminus ratio and cell volume decreased again. This highly dynamic and fast regulation has not yet been described for any other species. In this study, the gene dosage increase in origin-adjacent genes during the lag phase is discussed together with the nonrandom distribution of genes on the chromosomes of V. natriegens. Taken together, the results of this study provide the first comprehensive overview of the chromosome dynamics in V. natriegens and will guide the optimization of molecular biological characterization and biotechnological applications.
Unveiling the Oppressed Body: Female Dalit Body Politics in India through Baburao Bagul and Yashica Dutt
Authors: Cherechés Bianca
Published: July 2023

India’s complex social fabric is marked by a rigid caste system that has perpetuated discrimination and marginalisation for centuries. The caste structure not only establishes clear boundaries between castes through endogamous social relations, but also determines control over resources, productivity, and sexuality. Among the most vulnerable groups within this hierarchical structure are Dalit women, who face compounded forms of oppression due to their caste and gender, spanning economic, physical, and mental aspects. At the core of this oppression lies the Dalit woman’s body, a battleground where power dynamics intersect and the struggle for autonomy and dignity unfolds. This paper delves into the exploration of female Dalit body politics in India, with a particular focus on two influential literary works: Baburao Bagul’s When I Hid My Caste (2018) and Yashica Dutt’s Coming Out as Dalit (2019). The aim is to unveil, through these texts, the intersectionality of caste and gender, both past and present, revealing the violence, exploitation, and marginalisation that reflects on the Dalit female body, stemming from and affecting the economic, physical, and psychological dimension.
Using GHQ-12 to Screen Mental Health Issues in People with Emphysema
Authors: Kang Weixi , Whelan Edward , Pineda Hernández Sònia
Published: July 2023

Although previous studies have investigated the presence of psychometric comorbidities in individuals with emphysema, our understanding of the impact of emphysema on general mental health and specific dimensions of mental health, such as social dysfunction and anhedonia, depression and anxiety, and loss of confidence, remains limited. This research aims to examine the influence of emphysema on general mental health and its dimensions using the GHQ-12 assessment. By employing confirmatory factor analysis (CFA), the predictive normative approach, and one-sample t-test, we analyzed data from Understanding Society: the UK Household Longitudinal Study (UKHLS), including 97 individuals clinically diagnosed with emphysema and 8980 individuals without a clinical diagnosis of emphysema. The findings of this study indicate that individuals with clinically diagnosed emphysema experience poorer general mental health (t(96) = 8.41, p < 0.001, Cohen’s d = 0.12, 95% C.I. [0.09, 0.15]), increased levels of social dysfunction and anhedonia (t(96) = 6.02, p < 0.001, Cohen’s d = 0.09, 95% C.I. [0.06, 0.11]), heightened depression and anxiety (t(96) = 7.26, p < 0.001, Cohen’s d = 0.11, 95% C.I. [0.08, 0.14]), as well as elevated loss of confidence (t(96) = 6.40, p < 0.001, Cohen’s d = 0.09, 95% C.I. [0.07, 0.12]). These findings suggest the need for intervention programs aimed at improving the mental health of individuals with emphysema.
Effects of Sirolimus Treatment on Fetal Hemoglobin Production and Response to SARS-CoV-2 Vaccination: A Case Report Study
Authors: Gamberini Maria Rita , Zuccato Cristina , Zurlo Matteo , Cosenza Lucia Carmela , Finotti Alessia , Gambari Roberto
Published: July 2023

The β-thalassemias are a group of monogenic hereditary hematological disorders caused by deletions and/or mutations of the β-globin gene, leading to low or absent production of adult hemoglobin (HbA). For β-thalassemia, sirolimus has been under clinical consideration in two trials (NCT03877809 and NCT04247750). A reduced immune response to anti-SARS-CoV-2 vaccination has been reported in organ recipient patients treated with the immunosuppressant sirolimus. Therefore, there was some concern regarding the fact that monotherapy with sirolimus would reduce the antibody response after SARS-CoV-2 vaccination. In the representative clinical case reported in this study, sirolimus treatment induced the expected increase of fetal hemoglobin (HbF) but did not prevent the production of anti-SARS-CoV-2 IgG after vaccination with mRNA-1273 (Moderna). In our opinion, this case report should stimulate further studies on β-thalassemia patients under sirolimus monotherapy in order to confirm the safety (or even the positive effects) of sirolimus with respect to the humoral response to anti-SARS-CoV-2 vaccination. In addition, considering the extensive use of sirolimus for the treatment of other human pathologies (for instance, in organ transplantation, systemic lupus erythematosus, autoimmune cytopenia, and lymphangioleiomyomatosis), this case report study might be of general interest, as large numbers of patients are currently under sirolimus treatment.
Yield and Antioxidant Quality of Habanero Chili Pepper by Supplementing Potassium with Organic Products
Authors: González-Cortés Areli , Robledo-Torres Valentín , Luna-García Laura Raquel , Mendoza-Villarreal Rosalinda , Pérez-Rodríguez Miguel Ángel
Published: July 2023

Habanero chili pepper has great economic importance in Mexico, but its production is limited due to different factors that affect quality. Given the high demand and prices of the fruit, the production of this crop is spreading to other regions in search of new production systems. The aim of this research work was to assess the yield and antioxidant components of the fruit by supplementing potassium from organic sources. The “Campeche”, “Palenque”, and “Jaguar” varieties were studied in five NPK treatments; replacing the % of potassium with humus and compost: (T1) 240-200-120+50% of K from liquid earthworm humus; (T2) 240-200-180+25% of K from liquid earthworm humus; (T3) 240-200-120+50% of K from vermicompost; (T4) 240-200-180+25% of K from vermicompost; and (T5) 240-200-240 (control, 100% chemical nutrition). For yield components, there were significant differences, highlighting the importance of the nutrition source for the yield and weight of fruits per plant. The control (T5) presented a value of 39 t·ha−1 and was similar to treatments T2 and T3 with a supplement of 25 and 50% organic K. For the polar and equatorial diameter of fruit, as well as for plant height and leaf area, treatments supplemented with 50% organic K (T1 and T3) were more effective. For the varieties evaluated, no differences in yield and fruit weight were found; however, Campeche produced more fruits per plant with a greater equatorial diameter, while Palenque obtained fruits with a greater polar diameter and taller plants. The results of antioxidant compounds show that organic K supplementation improves the quality of total carotenoids, β-carotenes, and xanthophylls compared to 100% chemical fertilization, since total carotenoid content is improved by 54.2%, β-carotenes by 46.4%, and xanthophylls by 37.8%, respectively. The Campeche variety was the one that presented the best fruits with the highest antioxidant quality. These results indicate a positive effect of the combined application of chemical fertilizer with worm humus on yield and its components in pepper and other crops and show an economic, ecological, and sustainable alternative to the production of habanero chili pepper.
Superconducting Gravimeters: A Novel Tool for Validating Remote Sensing Evapotranspiration Products
Authors: Pendiuk Jonatan , Degano María Florencia , Guarracino Luis , Rivas Raúl Eduardo
Published: July 2023

The practical utility of remote sensing techniques depends on their validation with ground-truth data. Validation requires similar spatial-temporal scales for ground measurements and remote sensing resolution. Evapotranspiration (ET) estimates are commonly compared to weighing lysimeter data, which provide accurate but localized measurements. To address this limitation, we propose the use of superconducting gravimeters (SGs) to obtain ground-truth ET data at larger spatial scales. SGs measure gravity acceleration with high resolution (tenths of nm s−2) within a few hundred meters. Similar to lysimeters, gravimeters provide direct estimates of water mass changes to determine ET without disturbing the soil. To demonstrate the practical applicability of SG data, we conducted a case study in Buenos Aires Province, Argentina (Lat: −34.87, Lon: −58.14). We estimated cumulative ET values for 8-day and monthly intervals using gravity and precipitation data from the study site. Comparing these values with Moderate Resolution Imaging Spectroradiometer (MODIS)-based ET products (MOD16A2), we found a very good agreement at the monthly scale, with an RMSE of 32.6 mm month−1 (1.1 mm day−1). This study represents a step forward in the use of SGs for hydrogeological applications. The future development of lighter and smaller gravimeters is expected to further expand their use.
Physiological Effects of Visual Stimulation by a Japanese Low Wooden Table: A Crossover Field Experiment
Authors: Ikei Harumi , Jo Hyunju , Miyazaki Yoshifumi
Published: July 2023

The purpose of this study was to evaluate the physiological effects of visual stimulation by a unique Japanese low wooden table on the prefrontal cortex and autonomic nervous activities. A within-participants experiment with 26 male university students was conducted in a Japanese-style room. The visual stimuli were a low wooden table (WT) made of Japanese cypress and a low cloth-covered table (control) for an exposure time of 90 s. Near-infrared spectroscopy was used to measure the prefrontal cortex activity in the left and right prefrontal cortices as an indicator of oxyhemoglobin (oxy-Hb) concentration. Autonomic nervous activity was measured as an indicator of sympathetic (low-frequency/high-frequency component ratio, LF/HF), and parasympathetic (high-frequency components, HF) nervous activities were assessed by heart rate variability. Furthermore, the modified semantic differential method and the Profile of Mood States 2nd edition were used to measure psychological responses. Physiologically, the oxy-Hb concentration in the left prefrontal cortex and ln (LF/HF) were significantly lower during visual exposure to the WT than to the control. Psychologically, more comfortable, relaxed, and natural impressions, as well as improved mood states, were reported during visual stimulation to the WT than to the control. This study demonstrated that viewing a WT led to physiological relaxation and had a positive psychological effect on the participants.
Integrated Long-Term Care ‘Neighbourhoods’ to Support Older Populations: Evolving Strategies in Japan and England
Authors: Szczepura Ala , Masaki Harue , Wild Deidre , Nomura Toshio , Collinson Mark , Kneafsey Rosie
Published: July 2023

Western countries are currently facing the public health challenge of a rapidly aging population and the associated challenge of providing long-term care services to meet its needs with a reduced working age population. As people age, they will increasingly require both health and social care services to maintain their quality of life and these will need to be integrated to provide cost-effective long-term care. The World Health Organization recommended in 2020 that all countries should have integrated long-term care strategies to better support their older populations. Japan, with the most rapidly ageing society in the world, started to address this challenge in the 1990s. In 2017, it introduced a national policy for integrated long-term health and social care services at a local geographical level for older people. England has recently embarked on its first plan aiming for the integration of services for older people. In this article, we compare these approaches to the integration of long-term care systems, including the strengths of each. The paper also considers the effects of historical, cultural and organizational factors and the emerging role of technology. Finally, we identify critical lessons that can inform strategy development in other countries, and highlight the need to provide more international comparisons.
Evaluating the One-Year Impact of School e-Cigarette Use Interventions among Current Youth e-Cigarette Users in the COMPASS Study, 2017/18–2018/19
Authors: Cole Adam G. , Gohari Mahmood R. , Leatherdale Scott T.
Published: July 2023

There is a lack of evidence for the impact of school-based e-cigarette interventions among current e-cigarette users. This natural experimental evaluation study evaluated the one-year impact of various school-based e-cigarette prevention/cessation programs among a sample of current youth e-cigarette users. The COMPASS study sample included n = 3586 current e-cigarette users from n = 90 schools with data collected between 2017 and 2019. Student e-cigarette use patterns were categorized as “escalated”, “maintained”, and “reduced” based on the change in past 30-day e-cigarette use between baseline and follow-up. Intervention schools added e-cigarette use “prevention”, “cessation”, or “protection” programs, while control schools did not make any changes. Logistic regression models identified how each category of added programs was associated with e-cigarette use patterns. About one quarter of schools added an e-cigarette use prevention/cessation program over one year. Student e-cigarette use patterns between control and intervention groups differed in proportion ranging from a decrease of 3.35% to an increase of 5.80%. Regression models did not identify any significant differences in the odds of escalating or reducing e-cigarette use in intervention relative to control schools. While many schools implemented new e-cigarette programs over one year, none of the interventions led to significant changes in e-cigarette escalation or reduction among current youth e-cigarette users. Additional studies are needed to evaluate the impact of e-cigarette interventions among current e-cigarette users.
Wilderness Is the Prototype of Nature Regardless of the Individual’s Connection to Nature. An Empirical Verification of the Solastalgia Effect
Authors: Barbiero Giuseppe , Berto Rita , Senes Giulio , Fumagalli Natalia
Published: July 2023

(1) Background: Connectedness with Nature is a personality trait that influences our relationship with Nature. But Nature is not all the same. Wilderness is Nature in its original form, the form within which human beings have evolved as a species, while what we refer to as domesticated and urban Nature are relatively recent products of our interaction with the environment. (2) Aim: The main purpose of this study was to verify whether the individual trait “connection to Nature” influences the perception of restoration, preference for and familiarity with three types of Nature: wilderness, domesticated and urban. (3) Results: Regardless of the level of connection to Nature, wilderness is always perceived as more restorative than the domesticated or urban environment. Individuals with higher connectedness prefer wilderness more than others, and they are able to recognise the restorative value of domesticated environments more than those with medium or low levels of connectedness. Less connected individuals tend to prefer domesticated environments, although wilderness is more familiar to them. (4) Conclusions: This study shows that, despite our detachment from Nature, wilderness is the prototype of Nature, and this finding offers a plausible evolutionary explanation of solastalgia.
Beside the Seaside: Reflections on Local Green and Blue Spaces from Adults Aged over 50 in a Coastal Community
Authors: Pool Ursula , Kenyon Anna , Froggett Lynn , Dooris Mark
Published: July 2023

This qualitative study examined the perceptions of potential wellbeing benefits of local green and blue spaces for adults aged over 50 years in a coastal town in North West England. At a population level, living close to green and blue spaces is associated with better mental and physical health, with the strength of the benefits being inversely related to the economic prosperity of an area. However, living in economically disadvantaged coastal communities may be associated with poorer health and wellbeing, particularly for older adults, despite the proximity to blue (and often green) space. Exploring this apparent paradox was the aim of the present study. Through semi-structured interviews with members of a community group, we gained insight into lived experiences of local green and blue spaces. The main themes we developed from analysis of the conversations were accessibility, amenities, social connections, quality of environment, and recuperation and escape. Our findings illustrate that participants valued natural spaces that were local and accessible, particularly when they felt connected to them, and were less likely to visit spaces that were further away and that were perceived as being less welcoming or accessible. This study provides insights into the experiences of adults aged over 50 living in coastal areas and suggests that policies and interventions aimed at promoting wellbeing in this demographic should consider the value of hyper-local green and blue spaces and their potential to promote connectedness to nature.
Identifying the Production–Living–Ecological Functional Structure of Haikou City by Integrating Empirical Knowledge with Multi-Source Data
Authors: Zhao Bingbing , Tan Xiaoyong , Luo Liang , Deng Min , Yang Xuexi
Published: July 2023

The inefficient use of urban resources and the imbalance of spatial structures make optimizing land use management a top priority in urban environmental management. Traditional land use classification systems that prioritize only natural features while disregarding human activity can result in redundancy and conflicts in urban planning. The Production–Living–Ecological Space (PLES) approach was developed as an integrated method for territorial spatial classification. However, most existing studies on PLES are conducted at provincial scales, largely overlooking fine-scale usage within cities. In addition, the existing concept of PLES has been vaguely defined, resulting in linear and simple identification methods that are not applicable to complex urban environments. To address these issues, this study proposes a method to identify urban PLES based on supervised classification using random forest models, which integrate empirical knowledge and multi-source heterogeneous information. The experiments conducted in Haikou reveal the regional aggregation of living and production spaces and the scarcity of ecological space in the city. Our study proposes a concrete concept of PLES and a method for identifying PLES that can be applied to multiple regions, providing an effective tool for the coordinated management of urban production, living, and ecological environments.
Endothelial Senescence and Its Impact on Angiogenesis in Alzheimer’s Disease
Authors: Georgieva Irina , Tchekalarova Jana , Iliev Dimitar , Tzoneva Rumiana
Published: July 2023

Endothelial cells are constantly exposed to environmental stress factors that, above a certain threshold, trigger cellular senescence and apoptosis. The altered vascular function affects new vessel formation and endothelial fitness, contributing to the progression of age-related diseases. This narrative review highlights the complex interplay between senescence, oxidative stress, extracellular vesicles, and the extracellular matrix and emphasizes the crucial role of angiogenesis in aging and Alzheimer’s disease. The interaction between the vascular and nervous systems is essential for the development of a healthy brain, especially since neurons are exceptionally dependent on nutrients carried by the blood. Therefore, anomalies in the delicate balance between pro- and antiangiogenic factors and the consequences of disrupted angiogenesis, such as misalignment, vascular leakage and disturbed blood flow, are responsible for neurodegeneration. The implications of altered non-productive angiogenesis in Alzheimer’s disease due to dysregulated Delta-Notch and VEGF signaling are further explored. Additionally, potential therapeutic strategies such as exercise and caloric restriction to modulate angiogenesis and vascular aging and to mitigate the associated debilitating symptoms are discussed. Moreover, both the roles of extracellular vesicles in stress-induced senescence and as an early detection marker for Alzheimer’s disease are considered. The intricate relationship between endothelial senescence and angiogenesis provides valuable insights into the mechanisms underlying angiogenesis-related disorders and opens avenues for future research and therapeutic interventions.
An Ex Vivo Electroretinographic Apparatus for the mL-Scale Testing of Drugs to One Day and Beyond
Authors: Cangiano Lorenzo , Asteriti Sabrina
Published: July 2023

When screening new drugs to treat retinal diseases, ex vivo electroretinography (ERG) potentially combines the experimental throughput of its traditional in vivo counterpart, with greater mechanistic insight and reproducible delivery. To date, this technique was used in experiments with open loop superfusion and lasting up to a few hours. Here, we present a compact apparatus that provides continuous and simultaneous recordings of the scotopic a-waves from four mouse retinas for much longer durations. Crucially, each retina can be incubated at 37 °C in only 2 mL of static medium, enabling the testing of very expensive drugs or nano devices. Light sensitivity and response kinetics of these preparations remain in the physiological range throughout incubation, displaying only very slow drifts. As an example application, we showed that barium, a potassium channel blocker used to abolish the glial component of the ERG, displayed no overt side effects on photoreceptors over several hours. In another example, we fully regenerated a partially bleached retina using a minimal quantity of 9-cis-retinal. Finally, we demonstrated that including antibiotic in the incubation medium extends physiological light responses to over one day. This system represents a necessary stepping stone towards the goal of combining ERG recordings with organotypically cultured retinas.