bluuebunny/crossref_metadata_2025 huggingface dataset:
Battery Thermal Management Systems: Current Status and Design Approach of Cooling Technologies
Authors: Buidin Thomas Imre Cyrille , Mariasiu Florin
Published: August 2021

In the current context of transition from the powertrains of cars equipped with internal combustion engines to powertrains based on electricity, there is a need to intensify studies and research related to the command-and-control systems of electric vehicles. One of the important systems in the construction of an electric vehicle is the thermal management system of the battery with the role of optimizing the operation of the battery in terms of performance and life. The article aims to critically analyze the studies and research conducted so far related to the type, design and operating principles of battery thermal management systems (BTMSs) used in the construction of various shaped Li-ion batteries, with focus on cooling technologies. The advantages and disadvantages of the individual components, as well as of the proposed BTM solutions, are extensively investigated, with regard also to the adaptability of these systems to the different Li-ion battery shapes. The information thus synthesized provides the necessary and important information and proposes future directions in research to those interested in this topic to be used to increase the efficiency of the thermal management systems of the battery (and with it the global efficiency of the electric vehicle).
Numerical Investigation of Major Impact Factors Influencing Fracture-Driven Interactions in Tight Oil Reservoirs: A Case Study of Mahu Sug, Xinjiang, China
Authors: Yan Xiaolun , Mou Jianye , Tang Chuanyi , Xin Huazhi , Zhang Shicheng , Ma Xinfang , Duan Guifu
Published: August 2021

Fracture-driven interactions (FDIs) in unconventional reservoirs significantly affect well production and have thus garnered extensive attention from the scientific community. Furthermore, since the industry transitioned to using large completion designs with closer well spacing and infill drilling, FDIs have occurred more frequently and featured more prominently, which has primarily led to destructive interference. When infill wells (i.e., “child” wells) are fractured, older, adjacent producing wells (i.e., “parent” wells) are put directly at risk of premature changes in production behavior. Some wells may never fully recover following exposure to severe FDIs and, in the worst case scenario, will permanently stop producing. To date, previous investigations into FDIs have focused mainly on diagnosis and detection. As such, their formation mechanism is not well understood. To address this deficiency, a three-dimensional, multi-fracture propagation simulator was constructed based on the unconventional fracture model (UFM) and applied to a system that included both an older, adjacent passive well (“parent” well) and an active well (“child” well). Herein, the theoretical framework for overall complex fracture modeling is described. Furthermore, numerical simulation results are presented, demonstrating the critical roles of in-situ stress distribution and pre-existing natural fractures and aiding in the development of appropriate strategies for managing FDIs.
A Global Meta-Analysis for Estimating Local Ecosystem Service Value Functions
Authors: Magalhães Filho Luiz , Roebeling Peter , Bastos Maria Isabel , Rodrigues Waldecy , Ometto Giulia
Published: August 2021

The Meta-analysis has increasingly been used to synthesize the ecosystem services literature, with some testing of the use of such analyses to transfer benefits. These are typically based on local primary studies. However, meta-analyses associated with ecosystem services are a potentially powerful tool for transferring benefits, especially for environmental assets for which no primary studies are available. In this study we use the Ecosystem Service Valuation Database (ESVD), which brings together 1350 value estimates from more than 320 studies around the world, to estimate meta-regression functions for Provisioning, Regulating and maintenance, and Cultural ecosystem services across 12 biomes. We tested the reliability of these meta-regression functions and found that even using variables with high explanatory power, transfer errors could still be large. We show that meta-analytic transfer performs better than simple value transfer and, in addition, that local meta-analytical transfer (i.e., based on local explanatory variable values) provides more reliable estimates than global meta-analytical transfer (i.e., based on mean global explanatory variable values). Thus, we conclude that when taking into account the characteristics of the study area under analysis, including explanatory variables such as income, population density, and protection status, we can determine the value of ecosystem services with greater accuracy.
Application of Augmented Reality in the Sensory Evaluation of Yogurts
Authors: Dong Yanyu , Sharma Chetan , Mehta Annu , Torrico Damir D.
Published: August 2021

Augmented reality (AR) applications in the food industry are considered innovative to enrich the interactions among consumers, food products, and context. The study aimed to investigate the effects of AR environments on the sensory responses of consumers towards different yogurts. AR HoloLens headsets were used to set up two AR environments: (1) AR coconut view (ARC) and (2) AR dairy view (ARD). Hedonic ratings, just-about-right (JAR), check-all-that-apply (CATA) attribute terms, emotional responses, purchase intent, and consumer purchasing behaviors of three types of yogurts (dairy-free coconut, dairy, and mixed) were measured under ARC, ARD, and sensory booths (SB). The results showed that the liking scores of dairy and mixed yogurts were generally higher than the coconut yogurt regardless of the environment. The interaction effect of yogurts and environments was statistically significant in terms of appearance, taste/flavor, sweetness, mouthfeel, aftertaste, and overall liking. JAR and penalty analysis revealed that consumers penalized the coconut yogurt for being “too much” in sourness, “too little” in sweetness, and “too thin” in mouthfeel. For the CATA analysis, attribute terms positively associated with overall liking (such as “sweet”, “smooth”, and “creamy”) were selected for dairy and mixed yogurts, whereas the attribute terms negatively associated with overall liking (such as “firm”, “heavy”, and “astringent”) were only selected for coconut yogurts. Regarding yogurt-consumption behaviors, the purchase intent of dairy and mixed yogurts was higher than that of the coconut yogurt, and taste and health were considered to be the most critical factors for yogurt consumption.
Nutritional and Technological Optimization of Wheat-Chickpea- Milk Powder Composite Flour and Its Impact on Rheological and Sensorial Properties of Leavened Flat Bread
Authors: Benali Aouatif , En-nahli Youness , Noutfia Younès , Elbaouchi Adil , Kabbour Mohammed Rachid , Gaboun Fatima , El Maadoudi El Haj , Benbrahim Nadia , Taghouti Mona , Ouhssine Mohammed , Kumar Shiv
Published: August 2021

Flour quality is influenced by the nature of the gluten and its various components. Gluten free flour made of pulses is known to enhance the nutritional quality of wheat flour. However, its addition can compromise the rheological and sensorial attributes of the bread. We used mixture design to optimize nutritional and technological qualities of a wheat–chickpea flour blend by adding milk powder as a natural organoleptic improver. A total of thirteen flour blends were prepared by incorporating 10 to 30% chickpea flour and 10 to 20% milk powder to wheat flour. Our results showed that the optimal flour blend consisted of 60% wheat, 24% chickpea, and 16% milk powder. Farinographic parameters of the optimal dough blend remained on par with those of the control dough (100% wheat flour), thereby preserving its bread-making quality. Sensory analysis of breads made from the optimal flour blend revealed no significant difference (p ≤ 0.05) from wheat flour for crumb and chewiness. Appreciation was brought to the appearance, crust, aroma, and taste in the optimized bread. This study suggests that chickpea flour can be suitably incorporated into bread wheat flour up to a percentage of 24% with 16% milk powder to produce bread with optimal nutritional quality while improving its sensory attributes and consumer acceptability.
Study on the Enhancement of Immune Function of Astaxanthin from Haematococcus pluvialis
Authors: Fan Qingsheng , Chen Zhan , Wu Yating , Zhu Jiangxin , Yu Zhou
Published: August 2021

This study was aimed at investigating the effect of astaxanthin on the immune function and its safety in mice. It was administered once daily at low, medium and high doses (4.2, 8.35, 16.70 mg/kg BW) to mice for 30 days. Subsequently, the spleen and thymus index, spleen lymphocyte transformation activity, delayed allergy reaction, amounts of antibody-producing cells, half-hemolytic value HC50, carbon particle clearance rate, macrophage phagocytosis, and natural killer cell (NK) activity were determined. Acute oral toxicity and genotoxicity tests were conducted to evaluate the safety of astaxanthin. Compared with the control group, medium and high doses of astaxanthin significantly increased the proliferation and transformation activities of spleen lymphocytes, activities of antibody-producing cells, serum hemolysin levels, and carbon particle clearance rate in mice (phagocytic index). High doses significantly improved delayed allergy reaction and NK cell activity. Results of acute oral toxicity and genotoxicity tests were negative. Gross anatomical observations and histopathological examination showed no abnormal changes associated with the treatments. In the article, it is confirmed that astaxanthin treatments significantly improve immune functions and show no toxic effects in the experimental doses.
Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin
Authors: Zhang Kun , Wang Yanen , Wei Qinghua , Li Xinpei , Guo Ying , Zhang Shan
Published: August 2021

Tissue-engineered skin grafts have long been considered to be the most effective treatment for large skin defects. Especially with the advent of 3D printing technology, the manufacture of artificial skin scaffold with complex shape and structure is becoming more convenient. However, the matrix material used as the bio-ink for 3D printing artificial skin is still a challenge. To address this issue, sodium alginate (SA)/carboxymethyl cellulose (CMC-Na) blend hydrogel was proposed to be the bio-ink for artificial skin fabrication, and SA/CMC-Na (SC) composite hydrogels at different compositions were investigated in terms of morphology, thermal properties, mechanical properties, and biological properties, so as to screen out the optimal composition ratio of SC for 3D printing artificial skin. Moreover, the designed SC composite hydrogel skin membranes were used for rabbit wound defeat repairing to evaluate the repair effect. Results show that SC4:1 blend hydrogel possesses the best mechanical properties, good moisturizing ability, proper degradation rate, and good biocompatibility, which is most suitable for 3D printing artificial skin. This research provides a process guidance for the design and fabrication of SA/CMC-Na composite artificial skin.
Poor Lower Extremity Functioning Is Associated with Modest Increased Incidence of Probable Dementia
Authors: Teruya Sergio L. , Dimino Cara , Silverman Kevin D. , Mielenz Thelma
Published: August 2021

Lower extremity functioning in older adults provides a measure of poor physical performance and can predict negative health outcomes. The consequences of reduced lower extremity functioning on cognitive decline, measured as time-varying variables, have not been well documented in previous studies. We aimed to evaluate whether lower extremity functioning is associated with an increased incidence rate of probable dementia among older adults using data from the National Health and Aging Trends Study (NHATS). Participants (n = 6457) were followed for 8 years to examine the relationship between lower extremity functioning, as measured by the Short Physical Performance Battery (SPPB), and incident probable dementia. Using weighted data, a multivariable Poisson regression with generalized estimating equations (GEE) was used to calculate incidence rate ratios (IRR), adjusting for covariates and clustering. Participants with low SPPB scores (0–5) had a 5% increase in incident probable dementia when compared with those who had good SPPB scores (10–12) in the adjusted model (IRR = 1.05; 95% CI = 1.04–1.07). Lower extremity functioning is associated with a modest increase in incident probable dementia. The SPPB score may be helpful in identifying subjects at risk of dementia. Efforts aimed at improving physical functioning may lead to better cognitive outcomes.
Climate Change and Enteric Infections in the Canadian Arctic: Do We Know What’s on the Horizon?
Authors: Finlayson-Trick Emma , Barker Bronwyn , Manji Selina , Harper Sherilee L. , Yansouni Cedric P. , Goldfarb David M.
Published: August 2021

The Canadian Arctic has a long history with diarrheal disease, including outbreaks of campylobacteriosis, giardiasis, and salmonellosis. Due to climate change, the Canadian Arctic is experiencing rapid environmental transformation, which not only threatens the livelihood of local Indigenous Peoples, but also supports the spread, frequency, and intensity of enteric pathogen outbreaks. Advances in diagnostic testing and detection have brought to attention the current burden of disease due to Cryptosporidium, Campylobacter, and Helicobacter pylori. As climate change is known to influence pathogen transmission (e.g., food and water), Arctic communities need support in developing prevention and surveillance strategies that are culturally appropriate. This review aims to provide an overview of how climate change is currently and is expected to impact enteric pathogens in the Canadian Arctic.
Physiology Response and Resistance Evaluation of Twenty Coconut Germplasm Resources under Low Temperature Stress
Authors: Sun Chengxu , Zhang Ruining , Yuan Zhouyu , Cao Hongxing , Martin Jerome Jeyakumar John
Published: August 2021

Coconut (Cocos nucifera L.) is a tropical evergreen crop with high economic value. Low temperature is one of the main environmental factors that limit coconut productivity. Therefore, it is necessary and significant to research the growth trend and physiological changes of coconuts under a low temperature environment. In this study, the physiological response of 20 coconut germplasm resources is presented in an integrated perspective to provide a holistic view of the behavior of coconut trees facing cold stress under four temperature conditions (25 °C, 15 °C, 10 °C, 5 °C). It was shown that low temperature would lead to the increase of relative electrical conductivity, MDA content, soluble protein content, and proline content. In addition, the activities of defense enzymes (SOD, POD, CAT, APX) were increased to resist the cold environment. In a comprehensive analysis, it was revealed that coconut germplasms with high cold resistance, such as C2, C7, and C10 as well as POD activity, proline content, and soluble protein content, were defined as representatives for coconut cold resistance evaluation. Through the exploration of osmotic adjustment substances and defense enzymes, the breeding and quality improvement of cold-resistant coconut varieties could be promoted. As a result, understanding the physiological response and tolerance mechanisms of coconuts to low temperature stress was essential, as this perception may serve as the foundation for coconut resistance evaluation, cultivation, and breeding.
Effect of Drought Stress on Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities in Lettuce Seedlings
Authors: Shin Yu Kyeong , Bhandari Shiva Ram , Jo Jung Su , Song Jae Woo , Lee Jun Gu
Published: August 2021

This study monitored changes in chlorophyll fluorescence (CF), growth parameters, soil moisture content, phytochemical content (proline, ascorbic acid, chlorophyll, total phenol content (TPC), and total flavonoid content (TFC)), and antioxidant activities in 12-day-old lettuce (Lactuca sativa L.) seedlings grown under drought stress (no irrigation) and control (well irrigated) treatments in controlled conditions for eight days. Measurements occurred at two-day intervals. Among ten CF parameters studied, effective quantum yield of photochemical energy conversion in PSII (Y(PSII)), coefficient of photochemical quenching (qP), and coefficient of photochemical quenching of variable fluorescence based on the lake model of PSII (qL) significantly decreased in drought-stressed seedlings from day 6 of treatment compared to control. In contrast, maximum quantum yield (Fv/Fm), ratio of fluorescence (Rfd), and quantum yield of non-regulated energy dissipation in PSII (Y(NO)) were significantly affected only at the end. All growth parameters decreased in drought-stressed seedlings compared to control. Proline started increasing from day 4 and showed ~660-fold elevation on day 8 compared to control. Chlorophyll, ascorbic acid, TPC, TFC, and antioxidant activities decreased in drought-stressed seedlings. Results showed major changes in all parameters in seedlings under prolonged drought stress. These findings clarify effects of drought stress in lettuce seedlings during progressive drought exposure and will be useful in the seedling industry.
Comparative Growth, Photosynthetic Pigments, and Osmolytes Analysis of Hemp (Cannabis sativa L.) Seedlings under an Aeroponics System with Different LED Light Sources
Authors: Islam Md. Jahirul , Ryu Byeong Ryeol , Azad Md. Obyedul Kalam , Rahman Md. Hafizur , Rana Md. Soyel , Kang Chang-Won , Lim Jung-Dae , Lim Young-Seok
Published: August 2021

The performance of hemp seedlings was evaluated through morphological traits, photosynthetic pigments, and osmolytes under 11 light treatments (10 LED light compositions + natural light) in an aeroponics system. The seedlings were brought under treatment at 25 days of age, where the light intensity was 300 µmol m−2s−1 and duration was 20 days. A higher leaf number and node number were observed in L10 (R4:B2:W2:FR1:UV1) and L11 (R2:B2:G2:W2:FR1:UV1), and a higher leaf length and leaf width were recorded in the L2 (white), L3 (R8:B2), and L5 (R7:B2:FR1) treatments. Furthermore, a higher shoot length was recorded in L3 (R8:B2), L6 (R6:B2:G1:FR1), and L9 (R6:B2:FR1:UV1) while roots developed more in the L1 (natural light), L5 (R7:B2:FR1), and L9 (R6:B2:FR1:UV1) treatments. On the other hand, the L3 (R8:B2) treatment manifested higher chlorophyll a, chlorophyll b, and photosynthetic quantum yield (Fv/Fm). The hierarchical clustering and heatmap analysis revealed that higher leaf numbers and node numbers resulted in bushy plants with shorter shoots and longer roots. A negative correlation was also observed in photosynthetic traits (pigments and fluorescence) with osmolytes and root length. Importantly, the treatments L4 (R7:B2:G1), L6 (R6:B2:G1:FR1), L8 (R5:B2:G1:FR1:UV1), and L11 (R2:B2:G2:W2:FR1:UV1) manifested higher nodes with a higher osmolyte content, such as proline, ascorbic acid, total soluble carbohydrate, and sucrose, which may be a helpful indicator for higher branches and inflorescences, and ultimately higher cannabinoids accumulation in the plants. The approach and findings of this study could provide future research with the baseline information on optimizing the light composition to produce hemp plants with ideal phenotypes.
Effect of Acupuncture on Diabetic Neuropathy: A Narrative Review
Authors: Cho Eunwoo , Kim Woojin
Published: August 2021

Diabetic neuropathy, a major complication of diabetes mellitus, refers to a collection of clinically diverse disorders affecting the nervous system that may present with pain. Although the number of patients suffering from severe neuropathy is increasing, no optimal treatment method has been developed yet. Acupuncture is well known for its ability to reduce various kinds of pain, and a number of studies have also reported its effect on diabetes mellitus; however, its effect and underlying mechanism against diabetic neuropathy are not yet clearly understood. In this review, ten and five studies performed in humans and animals, respectively, were analyzed. All studies reported that acupuncture significantly relieved diabetic neuropathy. ST36, BL13, BL20, SP6, and SP9 were the most widely used acupoints. Five studies used electro-acupuncture, whereas other studies used manual acupuncture. Furthermore, the effect of acupuncture was shown to be mediated through the various molecules present in the peripheral nerves and spinal cord, such as P65, GPR78, and TRPV1. Five studies reported side effects, such as swelling, numbness, and nausea, but none were reported to be serious. Based on these results, we suggest that acupuncture should be considered as a treatment option for diabetic neuropathy.
Overexpression of ERAP2N in Human Trophoblast Cells Promotes Cell Death
Authors: Lospinoso Kristen , Dozmorov Mikhail , El Fawal Nadine , Raghu Rhea , Chae Wook-Jin , Lee Eun D.
Published: August 2021

The genes involved in implantation and placentation are tightly regulated to ensure a healthy pregnancy. The endoplasmic reticulum aminopeptidase 2 (ERAP2) gene is associated with preeclampsia (PE). Our studies have determined that an isoform of ERAP2-arginine (N), expressed in trophoblast cells (TC), significantly activates immune cells, and ERAP2N-expressing TCs are preferentially killed by both cytotoxic T lymphocytes (CTLs) and Natural Killer cells (NKCs). To understand the cause of this phenomenon, we surveyed differentially expressed genes (DEGs) between ERAP2N expressing and non-expressing TCs. Our RNAseq data revealed 581 total DEGs between the two groups. 289 genes were up-regulated, and 292 genes were down-regulated. Interestingly, most of the down-regulated genes of significance were pro-survival genes that play a crucial role in cell survival (LDHA, EGLN1, HLA-C, ITGB5, WNT7A, FN1). However, the down-regulation of these genes in ERAP2N-expressing TCs translates into a propensity for cell death. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that 64 DEGs were significantly enriched in nine pathways, including “Protein processing in endoplasmic reticulum” and “Antigen processing and presentation”, suggesting that the genes may be associated with peptide processes involved in immune recognition during the reproductive cycle.
Insulin Signal Transduction Perturbations in Insulin Resistance
Authors: Khalid Mariyam , Alkaabi Juma , Khan Moien A. B. , Adem Abdu
Published: August 2021

Type 2 diabetes mellitus is a widespread medical condition, characterized by high blood glucose and inadequate insulin action, which leads to insulin resistance. Insulin resistance in insulin-responsive tissues precedes the onset of pancreatic β-cell dysfunction. Multiple molecular and pathophysiological mechanisms are involved in insulin resistance. Insulin resistance is a consequence of a complex combination of metabolic disorders, lipotoxicity, glucotoxicity, and inflammation. There is ample evidence linking different mechanistic approaches as the cause of insulin resistance, but no central mechanism is yet described as an underlying reason behind this condition. This review combines and interlinks the defects in the insulin signal transduction pathway of the insulin resistance state with special emphasis on the AGE-RAGE-NF-κB axis. Here, we describe important factors that play a crucial role in the pathogenesis of insulin resistance to provide directionality for the events. The interplay of inflammation and oxidative stress that leads to β-cell decline through the IAPP-RAGE induced β-cell toxicity is also addressed. Overall, by generating a comprehensive overview of the plethora of mechanisms involved in insulin resistance, we focus on the establishment of unifying mechanisms to provide new insights for the future interventions of type 2 diabetes mellitus.
Soluble Receptor for Advanced Glycation End Products (sRAGE) Is a Sensitive Biomarker in Human Pulmonary Arterial Hypertension
Authors: Diekmann Franziska , Chouvarine Philippe , Sallmon Hannes , Meyer-Kobbe Louisa , Kieslich Moritz , Plouffe Brian D. , Murthy Shashi K. , Lichtinghagen Ralf , Legchenko Ekaterina , Hansmann Georg
Published: August 2021

Pulmonary arterial hypertension (PAH) is a progressive condition with an unmet need for early diagnosis, better monitoring, and risk stratification. The receptor for advanced glycation end products (RAGE) is activated in response to hypoxia and vascular injury, and is associated with inflammation, cell proliferation and migration in PAH. For the adult cohort, we recruited 120 patients with PAH, 83 with idiopathic PAH (IPAH) and 37 with connective tissue disease-associated PAH (CTD-PAH), and 48 controls, and determined potential plasma biomarkers by enzyme-linked immunoassay. The established heart failure marker NTproBNP and IL-6 plasma levels were several-fold higher in both adult IPAH and CTD-PAH patients versus controls. Plasma soluble RAGE (sRAGE) was elevated in IPAH patients (3044 ± 215.2 pg/mL) and was even higher in CTD-PAH patients (3332 ± 321.6 pg/mL) versus controls (1766 ± 121.9 pg/mL; p < 0.01). All three markers were increased in WHO functional class II+III PAH versus controls (p < 0.001). Receiver-operating characteristic analysis revealed that sRAGE has diagnostic accuracy comparable to prognostic NTproBNP, and even outperforms NTproBNP in the distinction of PAH FC I from controls. Lung tissue RAGE expression was increased in IPAH versus controls (mRNA) and was located predominantly in the PA intima, media, and inflammatory cells in the perivascular space (immunohistochemistry). In the pediatric cohort, plasma sRAGE concentrations were higher than in adults, but were similar in PH (n = 10) and non-PH controls (n = 10). Taken together, in the largest adult sRAGE PAH study to date, we identify plasma sRAGE as a sensitive and accurate PAH biomarker with better performance than NTproBNP in the distinction of mild PAH from controls.
Effect of Neprilysin Inhibition for Ischemic Mitral Regurgitation after Myocardial Injury
Authors: Lee Sahmin , Hwang Hyo-Sook , Song Naaleum , Kang Geun-Hyung , Choi Kyoung-Hee , Ji Eunhye , Song Jong-Min , Kang Duk-Hyun
Published: August 2021

Angiotensin receptor neprilysin inhibitor (ARNI) treatment reduces functional mitral regurgitation (MR) to a greater extent than angiotensin receptor blocker (ARB) treatment alone, but the mechanism is unclear. We evaluated the mechanisms of how ARNI has an effect on functional MR. After inducing functional MR by left circumflex coronary artery occlusion, male Sprague Dawley rats (n = 31) were randomly assigned to receive the ARNI LCZ696, the ARB valsartan, or corn oil only (MR control). Excised mitral leaflets and left ventricle (LV) were analyzed, and valvular endothelial cells were evaluated focusing on molecular changes. LCZ696 significantly attenuated LV dilatation after 6 weeks when compared with the control group (LV end-diastolic volume, 461.3 ± 13.8 µL versus 525.1 ± 23.6 µL; p < 0.05), while valsartan did not (471.2 ± 8.9 µL; p > 0.05 to control). Histopathological analysis of mitral leaflets showed that LCZ696 strongly reduced fibrotic thickness compared to the control group (28.2 ± 2.7 µm vs. 48.8 ± 7.5 µm; p < 0.05). Transforming growth factor-β and downstream phosphorylated extracellular-signal regulated kinase were also significantly lower in the LCZ696 group. Consequently, excessive endothelial-to-mesenchymal transition (EndoMT) was mitigated in the LCZ696 group compared to the control group and leaflet area was higher (11%) in the LCZ696 group than in the valsartan group. Finally, the MR extent was significantly lower in the LCZ696 group and functional improvement was observed. In conclusion, neprilysin inhibitor has positive effects on LV reverse remodeling and also attenuates fibrosis in MV leaflets and restores adaptive growth by directly modulating EndoMT.
A Time-Course Study of the Expression Level of Synaptic Plasticity-Associated Genes in Un-Lesioned Spinal Cord and Brain Areas in a Rat Model of Spinal Cord Injury: A Bioinformatic Approach
Authors: Baldassarro Vito Antonio , Sanna Marco , Bighinati Andrea , Sannia Michele , Gusciglio Marco , Giardino Luciana , Lorenzini Luca , Calzà Laura
Published: August 2021

“Neuroplasticity” is often evoked to explain adaptation and compensation after acute lesions of the Central Nervous System (CNS). In this study, we investigated the modification of 80 genes involved in synaptic plasticity at different times (24 h, 8 and 45 days) from the traumatic spinal cord injury (SCI), adopting a bioinformatic analysis. mRNA expression levels were analyzed in the motor cortex, basal ganglia, cerebellum and in the spinal segments rostral and caudal to the lesion. The main results are: (i) a different gene expression regulation is observed in the Spinal Cord (SC) segments rostral and caudal to the lesion; (ii) long lasting changes in the SC includes the extracellular matrix (ECM) enzymes Timp1, transcription regulators (Egr, Nr4a1), second messenger associated proteins (Gna1, Ywhaq); (iii) long-lasting changes in the Motor Cortex includes transcription regulators (Cebpd), neurotransmitters/neuromodulators and receptors (Cnr1, Gria1, Nos1), growth factors and related receptors (Igf1, Ntf3, Ntrk2), second messenger associated proteins (Mapk1); long lasting changes in Basal Ganglia and Cerebellum include ECM protein (Reln), growth factors (Ngf, Bdnf), transcription regulators (Egr, Cebpd), neurotransmitter receptors (Grin2c). These data suggest the molecular mapping as a useful tool to investigate the brain and SC reorganization after SCI.
Heparin–Avastin Complexes Show Enhanced VEGF Binding and Inhibition of VEGF-Mediated Cell Migration
Authors: Tsiros Divyabharathy , Sheehy Casey E. , Nugent Matthew A.
Published: August 2021

Bevacizumab (known by the tradename Avastin) is an antibody that binds VEGF and blocks its binding to VEGF receptors on endothelial cells, and is used to treat cancers and other diseases associated with excessive vascular growth. Our previous findings showed enhanced VEGF binding to Avastin in the presence of heparin, indicating that colocalizing heparin with Avastin could enhance VEGF inhibitory activity. Thus, the aim of the present study was to determine if conjugating Avastin and heparin to one another would lead to enhanced anti-VEGF activity. Avastin was conjugated to either biotin or streptavidin, and biotin–heparin was used to bring the two molecules into close proximity via biotin–streptavidin binding. Radioligand binding assays with 125 I-VEGF and cell migration assays using human umbilical vein endothelial cells were used to evaluate the impact of heparin on Avastin binding and activity. We found that bringing Avastin and heparin together, either on a surface or through streptavidin conjugation of Avastin, led to increased VEGF binding compared to that with each molecule alone. The heparin-mediated increase in VEGF binding was also noted at acidic pH where Avastin showed decreased VEGF binding. Conditions where Avastin and heparin showed enhanced VEGF binding also showed reduced VEGF-induced migration of human umbilical vein endothelial cells. These findings suggest design principles for a modified Avastin-based inhibitor of angiogenesis.
Sleep Habits during COVID-19 Confinement: An Exploratory Analysis from Portugal
Authors: Silva Ema Pinto , Sobral Sónia Rolland
Published: August 2021

COVID-19 pandemic consequences are tragic, and many problems will persist after the health problem ends. Some studies have focused on mental health issues, reporting worrying percentages. It is known that there is a bidirectional relationship between mental health and sleep quality, and it would be expected that sleep would be affected by the pandemic. In order to know the Portuguese people’s habits before and during the confinement, we carried out a survey of 188 people aged 13 to 84 (38 ± 15) to find out the most frequent sleep patterns, habits and disorders before and during confinement. With this survey it was also intended to measure the most frequent changes in sleep patterns, habits, and disturbances on the general population and according to demographic data (gender, age group and professional status), sleep disorders arise, perceptions about sleep during confinement and if Portuguese think that in the future the sleep patterns will be the pre or during outbreak. Results indicate that, comparing before and during confinement, there is a slight correlation between gender and sleep disorders (before vs. during), a correlation between age group and professional status time to wake up, and between professional status and sleep disorders, and a strong correlation between the professional situation and changes in the invigorated feeling level (p < 0.001). Support for mental health and interventions to improve sleep quality should be offered to the population in general, and, according to our study, the Portuguese population.
Changes in Daily Life Satisfaction among Community Dwelling Elderly during the COVID-19 Pandemic in Japan
Authors: Ishida Akira , Ishida Emiko
Published: August 2021

Previous studies pointed out that the outbreak of the coronavirus disease 2019 (COVID-19) had a lesser effect on elderly people compared to their younger counterparts. However, a limited number of studies have analyzed the effects of COVID-19 on the psychological aspects of the elderly using longitudinal data. Therefore, the main objective of our study was to clarify the changes in self-rated overall daily life satisfaction among community dwelling elderly in Japan during the COVID-19 pandemic. We used the longitudinal data of 5176 individuals aged 15 years and older from the first and second internet surveys on “Changes in Daily Life Awareness and Behavior under the Influence of the New Coronavirus Infection” conducted by the Cabinet Office of Japan. The estimation result of the mixed-effects model indicated that elderly people were more adversely affected by the first wave of the pandemic (May 2020) compared to their younger counterparts. However, by the beginning of the third wave (December 2020), they had largely recovered to the same level as that of before the COVID-19 pandemic, which suggested that the elderly managed to cope with psychological distress to some extent during the prolonged COVID-19 pandemic.
Impact of a Breathing Intervention on Engagement of Abdominal, Thoracic, and Subclavian Musculature during Exercise, a Randomized Trial
Authors: Bahenský Petr , Bunc Václav , Malátová Renata , Marko David , Grosicki Gregory J. , Schuster Jan
Published: August 2021

Background: Breathing technique may influence endurance exercise performance by reducing overall breathing work and delaying respiratory muscle fatigue. We investigated whether a two-month yoga-based breathing intervention could affect breathing characteristics during exercise. Methods: Forty-six endurance runners (age = 16.6 ± 1.2 years) were randomized to either a breathing intervention or control group. The contribution of abdominal, thoracic, and subclavian musculature to respiration and ventilation parameters during three different intensities on a cycle ergometer was assessed pre- and post-intervention. Results: Post-intervention, abdominal, thoracic, and subclavian ventilatory contributions were altered at 2 W·kg−1 (27:23:50 to 31:28:41), 3 W·kg−1 (26:22:52 to 28:31:41), and 4 W·kg−1 (24:24:52 to 27:30:43), whereas minimal changes were observed in the control group. More specifically, a significant (p < 0.05) increase in abdominal contribution was observed at rest and during low intensity work (i.e., 2 and 3 W·kg−1), and a decrease in respiratory rate and increase of tidal volume were observed in the experimental group. Conclusions: These data highlight an increased reliance on more efficient abdominal and thoracic musculature, and less recruitment of subclavian musculature, in young endurance athletes during exercise following a two-month yoga-based breathing intervention. More efficient ventilatory muscular recruitment may benefit endurance performance by reducing energy demand and thus optimize energy requirements for mechanical work.
Disease Manifestations in Mucopolysaccharidoses and Their Impact on Anaesthesia-Related Complications—A Retrospective Analysis of 99 Patients
Authors: Ammer Luise Sophie , Dohrmann Thorsten , Muschol Nicole Maria , Lang Annika , Breyer Sandra Rafaela , Ozga Ann-Kathrin , Petzoldt Martin
Published: August 2021

Patients with mucopolysaccharidoses (MPS) frequently require anaesthesia for diagnostic or surgical interventions and thereby experience high morbidity. This study aimed to develop a multivariable prediction model for anaesthesia-related complications in MPS. This two-centred study was performed by retrospective chart review of children and adults with MPS undergoing anaesthesia from 2002 until 2018. We retrieved the patients’ demographics, medical history, clinical manifestations, and indication by each anaesthesia. Multivariable mixed-effects logistic regression was calculated for a clinical model based on preoperative predictors preselected by lasso regression and another model based on disease subtypes only. Of the 484 anaesthesia cases in 99 patients, 22.7% experienced at least one adverse event. The clinical model resulted in a better forecast performance than the subtype-model (AICc 460.4 vs. 467.7). The most relevant predictors were hepatosplenomegaly (OR 3.10, CI 1.54–6.26), immobility (OR 3.80, CI 0.98–14.73), and planned major surgery (OR 6.64, CI 2.25–19.55), while disease-specific therapies, i.e., haematopoietic stem cell transplantation (OR 0.45, CI 0.20–1.03), produced a protective effect. Anaesthetic complications can best be predicted by surrogates for advanced disease stages and protective therapeutic factors. Further model validation in different cohorts is needed.
Computational Investigation of Crack-Induced Hot-Spot Generation in Energetic Composites
Authors: Yang Xingzi , Lin Liqiang , Wilkerson Justin , Zeng Xiaowei
Published: August 2021

The sensitivity of polymer-bonded explosives (PBXs) can be tuned through adjusting binder material and its volume fraction, crystal composition and morphology. To obtain a better understanding of the correlation between grain-level failure and hot-spot generation in this kind of energetic composites as they undergo mechanical and thermal processes subsequent to impact, a recently developed interfacial cohesive zone model (ICZM) was used to study the dynamic response of polymer-bonded explosives. The ICZM can capture the contributions of deformation and fracture of the binder phase as well as interfacial debonding and subsequent friction on hot-spot generation. In this study, a two-dimensional (2D) finite element (FE) computational model of energetic composite was developed. The proposed computational model has been applied to simulate hot-spot generation in polymer-bonded explosives with different grain volume fraction under dynamic loading. Our simulation showed that the increase of binder phase material volume fraction will decrease the local heat generation, resulting in a lower temperature in the specimen.
A Comprehensive Risk Assessment Framework for Inland Waterway Transportation of Dangerous Goods
Authors: Huang Xi , Wen Yuanqiao , Zhang Fan , Sui Zhongyi , Cheng Xiaodong
Published: August 2021

A framework for risk assessment due to inland waterway transportation of dangerous goods is designed based on all possible event types that may be caused by the inland transportation of dangerous goods. The objective of this study is to design a framework for calculating the risks associated with changes in the transportation of dangerous goods along inland waterways. The framework is based on the traditional definition of risk and is designed for sensitive riverside environmental conditions in inland waterways. From the perspective of transportation management, this paper introduced the concept of transportability of dangerous goods and constructed a transportability assessment framework, which consists of a multi-index evaluation system and a single metric model. The result of the assessment is as an auxiliary basis to determine the transportation permit and control intensity of dangerous goods in an inland waterway specific voyage. The methodology is illustrated using a case study of transporting fireworks in the Yangtze River.
Assessment of and Adaptation to Beach Erosion in Islands: An Integrated Approach
Authors: Andreadis Olympos , Chatzipavlis Antonis , Hasiotis Thomas , Monioudi Isavela , Manoutsoglou Evangelia , Velegrakis Adonis
Published: August 2021

Island beaches, which form significant natural and economic resources, are under increasing erosion risk due to sea level rise. The present contribution proposes an integrated methodological framework for the evaluation of the socio-economic significance of beaches and their vulnerability to sea level rise and the design of effective adaptation measures. The approach comprises four steps: (i) beach ranking on the basis of their socio-economic significance and vulnerability in order to prioritize adaptation responses; (ii) monitoring of the hydro- and morphodynamic regime of the most highly ranking beaches using field observations and modelling, (iii) assessment of the sediment volumes required for beach nourishment under different scenarios of sea level rise and nourishment designs; (iv) evaluation of the marine aggregate potential of the adjacent areas that can be used for beach nourishment. The framework was applied to the Greek island of Chios, which has many beaches that are already under erosion. The methodology was shown to provide a structured approach for the assessment and response to erosion of the most vulnerable beach.
Patient-Centered Care for Patients with Depression or Anxiety Disorder: An Integrative Review
Authors: Pinho Lara Guedes de , Lopes Manuel José , Correia Tânia , Sampaio Francisco , Arco Helena Reis do , Mendes Artur , Marques Maria do Céu , Fonseca César
Published: August 2021

People have specific and unique individual and contextual characteristics, so healthcare should increasingly opt for person-centered care models. Thus, this review aimed to identify and synthesize the indicators for the care process of the person with depression and/or anxiety disorders, based on patient-centered care, going through the stages of diagnostic assessment and care planning, including intervention. An integrative literature review with research in seven scientific databases and a narrative analysis were carried out. Twenty articles were included, with indicators for diagnostic evaluation and care/intervention planning being extracted. Care planning focused on people with depression and/or anxiety disorder must be individualized, dynamic, flexible, andparticipatory. It must respond to the specific needs of the person, contemplating the identification of problems, the establishment of individual objectives, shared decision making, information and education, systematic feedback, and case management, and it should meet the patient’s preferences and satisfaction with care and involve the family and therapeutic management in care. The existence of comorbidities reinforces the importance of flexible and individualized care planning in order to respond to the specific health conditions of each person.
Augmented-Reality-Assisted K-Wire Placement for Glenoid Component Positioning in Reversed Shoulder Arthroplasty: A Proof-of-Concept Study
Authors: Schlueter-Brust Klaus , Henckel Johann , Katinakis Faidon , Buken Christoph , Opt-Eynde Jörg , Pofahl Thorsten , Rodriguez y Baena Ferdinando , Tatti Fabio
Published: August 2021

The accuracy of the implant’s post-operative position and orientation in reverse shoulder arthroplasty is known to play a significant role in both clinical and functional outcomes. Whilst technologies such as navigation and robotics have demonstrated superior radiological outcomes in many fields of surgery, the impact of augmented reality (AR) assistance in the operating room is still unknown. Malposition of the glenoid component in shoulder arthroplasty is known to result in implant failure and early revision surgery. The use of AR has many promising advantages, including allowing the detailed study of patient-specific anatomy without the need for invasive procedures such as arthroscopy to interrogate the joint’s articular surface. In addition, this technology has the potential to assist surgeons intraoperatively in aiding the guidance of surgical tools. It offers the prospect of increased component placement accuracy, reduced surgical procedure time, and improved radiological and functional outcomes, without recourse to the use of large navigation or robotic instruments, with their associated high overhead costs. This feasibility study describes the surgical workflow from a standardised CT protocol, via 3D reconstruction, 3D planning, and use of a commercial AR headset, to AR-assisted k-wire placement. Post-operative outcome was measured using a high-resolution laser scanner on the patient-specific 3D printed bone. In this proof-of-concept study, the discrepancy between the planned and the achieved glenoid entry point and guide-wire orientation was approximately 3 mm with a mean angulation error of 5°.
Long- and Short-Term Exposures to PM10 Can Shorten Telomere Length in Individuals Affected by Overweight and Obesity
Authors: Carugno Michele , Borroni Elisa , Fedrizzi Luca , Hoxha Mirjam , Vigna Luisella , Consonni Dario , Bollati Valentina , Pesatori Angela Cecilia
Published: August 2021

Reduced telomere length (TL) has been associated with increased risk of age-related diseases, most likely through oxidative stress and inflammation, which have also been claimed as mechanisms underlying health effects of air pollution exposure. We aimed to verify whether exposure to particulate matter with diameter ≤10 µm (PM10) affects TL. We recruited 1792 participants with overweight/obesity in Milan (Italy) in 2010–2015 who completed a structured questionnaire on sociodemographic data, gave a blood sample for TL measurement by real-time PCR, and were assigned air pollution and meteorological data of their residential address. In multivariate mixed-effects linear models (with a random intercept on PCR plate), we observed a −0.51% change in TL (95% confidence interval (CI): −0.98; −0.05)) per 10 µg/m3 increase in PM10 at the day of recruitment. A similar decreasing trend in TL was observed up to two weeks before withdrawal, with percentage changes as low as −1.53% (average exposure of the 12 days before recruitment). Mean annual exposure to PM10 was associated with −2.57% TL reduction (95%CI: −5.06; −0.08). By showing consistent associations between short- and long-term PM10 exposures and reduced TL, our findings shed light on the potential mechanisms responsible for the excess of age-related diseases associated with air pollution exposure.