bluuebunny/crossref_metadata_2025 huggingface dataset:
Addition de lipides protégés (encapsulés ou savons de calcium) à la ration de vaches laitières. Effets sur les performances et la composition du lait
Authors: CHILLIARD Y. , DOREAU M. , GAGLIOSTRO G. , ELMEDDAH Y.
Published: July 2020

L’une des limites à l’utilisation des matières grasses dans l’alimentation des ruminants tient aux perturbations qu’elles engendrent dans la digestion des glucides dans le rumen. Pour éviter cet inconvénient, diverses formes de protection des lipides sont apparues. Cet article récapitule les effets de l’addition des graisses animales (en moyenne 941 g/j) et d’huiles végétales (693 g/j) encapsulées, et de savons de calcium (593 g/j), dans la ration des vaches laitières sur les quantités ingérées, la production et la composition du lait. L’addition de matières grasses protégées entraîne une diminution des quantités ingérées plus faible pour les savons de calcium (0,55 kg MS/jour) que pour les graisses ou les huiles encapsulées (1,1 à 1,3 kg MS/jour). Ces diminutions sont comparables à celles observées avec des matières grasses non protégées. L’incorporation de graisses encapsulées ou de savons de calcium accroît la production laitière d’environ 1 kg/jour, alors que celle d’huiles encapsulées ne la modifie pas. L’addition de graisses encapsulées ou de savons de calcium réduit le taux protéique de 1,8 et 1,2 g/kg, respectivement, alors que les huiles encapsulées ne le modifient pas. Cette différence est une conséquence des variations de production laitière, la quantité de protéines sécrétée n’étant modifiée par aucun des trois suppléments. L’encapsulation des lipides entraîne un accroissement important du taux butyreux avec les graisses (4,0 g/kg) et les huiles (6,4 g/kg). L’augmentation de sécrétion d’acides gras longs est en effet supérieure à la réduction de la synthèse de novo d’acides gras courts et moyens. L’accroissement variable de la sécrétion d’acides gras polyinsaturés reflète l’efficacité de la protection contre l’hydrogénation ruminale. L’addition de savons de calcium n’accroît pas le taux butyreux ; selon les essais, l’inhibition de la synthèse de novo est compensée par une sécrétion accrue soit d’acide palmitique, soit d’acide oléique.
Description de la base de données "LASCAR", un outil pour l’étude des carrières des vaches laitières
Authors: COULON J.B. , LESCOURRET F. , FAYE B. , LANDAIS E. , TROCCON J.L. , PÉROCHON L.
Published: July 2020

Dans l’objectif d’étudier la carrière des vaches laitières (à partir du premier vêlage), une base de données relationnelle, baptisée LASCAR, a été élaborée. Elle a été alimentée à partir d’enregistrements réalisés au cours des 20 dernières années dans les troupeaux laitiers des 3 domaines expérimentaux du centre INRA de Clermont-Fd-Theix. Elle est composée de 10 tables décrivant les caractéristiques génétiques et de la période d’élevage des animaux, les performances individuelles à différentes échelles de temps (production et composition du lait, poids vif, troubles sanitaires, événements liés à la reproduction), et quelques facteurs de variation de ces performances (conduite alimentaire, climat). Elle regroupe 3851 lactations provenant de 1179 carrières. Ses données ont fait l’objet d’un contrôle de cohérence exhaustif. Différentes démarches d’étude de la carrière à partir de cette base sont présentées.
Les phytases fongiques dans l’alimentation des volailles
Authors: SAUVEUR B.
Published: July 2020

résumé non disponible pour cet article
Performances des vaches laitières en alpage : effet du niveau de la complémentation en aliment concentré
Authors: HAUWUY A. , BORNARD A. , COULON J.B. , HALTEL L.
Published: July 2020

Soixante dix-neuf vaches de race Tarentaise ont été utilisées au cours d’un essai de complémentation à l’alpage conduit 2 années consécutives. Au cours de la période estivale, tous les animaux pâturaient sur un alpage des Alpes du Nord, d’altitude comprise entre 1650 et 2200 m, conduit en pâturage rationné (renouvellement des parcs 2 fois par jour). Chaque année, au cours de l’ensemble de la période d’alpage, 2 lots ont été constitués : les vaches du lot Haut recevaient en moyenne 1,5 kg/j de concentré de plus que celles du lot Bas. L’effet du niveau des apports de concentré a été identique au cours des 2 étés : les vaches du lot Haut ont produit en moyenne 1,1 kg/j de lait de plus que celles du lot Bas (P<0,01), leur taux protéique a été légèrement plus élevé (+ 0,8 g/kg) et elles ont repris plus de poids vif (+ 6 kg) et d’état corporel (+ 0,4 point d’état) que celles du lot Bas. La reconstitution des réserves corporelles a été particulièrement faible chez les vaches du lot Bas en début de lactation à la montée à l’alpage. Ces résultats ont été comparés à ceux obtenus en zone de plaine, de demi-montagne ou sur d’autres alpages des Alpes du Nord.
L’élevage en Guyane. Bilan des travaux réalisés sur les espèces domestiques et sur deux espèces sauvages autochtones
Authors: INGRAND S. , HOSTACHE G.
Published: July 2020

Les travaux à caractère zootechnique réalisés par l’INRA sur sa station expérimentale dans le cadre du Plan Vert, ont surtout concerné l’élevage bovin à viande (zébu Brahman). Ils ont permis l’acquisition de résultats portant sur les performances de reproduction en milieu équatorial, et la conduite des taurillons à l’herbe entre le sevrage (âge de 9 à 10 mois) et l’abattage (âge de 24 à 30 mois). Parallèlement, entre 1985 et 1987, des suivis de troupeaux privés ont permis l’analyse des résultats techniques des élevages locaux, ainsi que l’ébauche d’une typologie des exploitations basée sur les pratiques des éleveurs. Plusieurs enquêtes, ainsi que des études par filières (porcine, avicole, cunicole) ont également montré que, relativement à l’ensemble de l’activité agricole, la production de viande bovine était loin d’être prédominante en Guyane. Une caractéristique importante des exploitations pratiquant l’élevage s’est avérée être la diversification de leurs spéculations, gage de souplesse, de capacité d’adaptation au marché et aux besoins de trésorerie, et donc de leur pérennité. D’autre part, l’élevage de certaines espèces sauvages autochtones pourraient, dans ce contexte de diversification des spéculations, constituer une alternative intéressante par la valorisation des ressources biologiques naturelles de la Guyane. Deux espèces ont déjà fait l’objet d’études : un mammifère rongeur, le cabiaï (Hydrochoerus hydrochaeris) et un poisson d’eau douce, l’atipa (Hoplosternum littorale). Illustrées par ces exemples, quelques pistes de réflexion sont présentées concernant les conditions d’émergence de systèmes d’élevages pérennes en Guyane. Elles soulignent notamment l’importance de la prise en compte des aspects autres que techniques (principalement économiques et sociologiques) pour l’appui au développement de l’élevage dans le contexte très particulier de la Guyane.
Effets de l’alimentation hivernale des génisses sur les performances et la longévité des vaches
Authors: TROCCON J.L.
Published: July 2020

Des génisses laitières de race Pie-noir nées à l’automne (n=171), destinées à un vêlage vers 2 ans, ont reçu des niveaux alimentaires HAUT (H) ou BAS (B) au cours des 2 périodes hivernales de leur phase d’élevage : de la naissance à 6 mois (H1 et B1) et entre 12 et 18 mois (H2 et B2). Dans les lots H1 et H2 les apports énergétiques étaient plus élevés de 22 % (100 UFL) et de 49 % (400 UFL) par rapport aux lots B1 et B2. Les gains de poids vif ont été de 826 g/j dans le lot H1, 696 g/j dans le lot B1, 885 g/j dans le lot H2 et 639 g/j dans le lot B2. Les conduites estivales au pâturage ont permis une croissance compensatrice modérée (50 à 55 %). Les traitements alimentaires n’ont pas d’effet sur les productions laitières par lactation. Le traitement H1 accroît la durée de vie productive des vaches (+ 333 jours) et les productions de lait (+ 4809 kg), de matières grasses (+ 205 kg) et de matières protéiques (+ 150 kg). Les vaches de ce lot produisent plus de veaux (+ 0,9) et des carcasses plus lourdes à la réforme (+ 20 kg). Le traitement H2 limite les difficultés au premier vêlage. La mortalité des vaches et la fréquence des mammites sont moindres dans les lots H1 et H2. Sur la base de ces résultats, le poids vif recommandé pour des génisses à l’âge de 6 mois représenterait 30 % du poids adulte des vaches. Les objectifs pondéraux pour les génisses seraient de 60 % du poids adulte des vaches à l’insémination (15 mois) et 90 % avant le premier vêlage ( à 24 mois).
KARAKTERISTIK TABLET EFFERVESCENT LABU SIAM (Sechium edule Sw.) DAN EKSTRAK SECANG (Caesalpinia sappan L.) DENGAN FILLER LAKTOSA-MANITOL
Authors: Sulistiani Natalia Datik , Anam Choirul , Yudhistira Bara
Published: July 2020

Produk effervescent disukai konsumen karena memiliki keuntungan yaitu praktis, mudah dilarutkan dan mempunyai sensasi menyegarkan ketika diminum. Dalam pembuatannya, bahan pengisi juga menentukan karakteristik tablet yang dihasilkan. Bahan utama yang bisa digunakan dalam pembuatan effervescent yaitu labu siam dan ekstrak secang. Labu siam maupun secang mempunyai senyawa antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh bahan pengisi yaitu laktosa-manitol terhadap sifat kimia, fisik granul, fisik tablet serta sensoris effervescent labu siam dan ekstrak secang. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu penggunaan laktosa-manitol sebagai bahan pengisi dengan variasi konsentrasi laktosa-manitol sebesar 100:0%, 80:20%; 50:50%; 20:80% dan 0:100%. Data yang diperoleh dianalisis statistik dengan metode One Way Analysis of Varians (ANOVA). Apabila menunjukkan hasil yang siginifikan, maka dilakukan pengujian lanjutnya dengan menggunakan analisis Duncan’s Multiple Range Test (DMRT) pada taraf signifikansi α=0,05. Hasil menunjukan penggunaan laktosa-manitol sebagai bahan pengisi berpengaruh terhadap porositas, kompresibilitas, kecepatan alir, sudut diam granul, kekerasan, kerapuhan, waktu larut tablet, warna dan kenampakan tablet serta rasa dan overall minuman effervescent, namun tidak berpengaruh secara signifikan terhadap kadar air, pH, aktivitas antioksidan, kenampakan dan aroma minuman effervescent. Formulasi terpilih berdasarkan uji pembobotan yaitu effervescent labu siam dan ekstrak secang dengan pengisi laktosa 20% : manitol 80% dimana memiliki aktivitas antioksidan sebesar 3,385% dan waktu larut tablet 1,779 menit.
latihan JTHP
Authors: To Boy
Published: July 2020

latihan
KARAKTERISTIK BROWNIS COKELAT KUKUS BERBAHAN DASAR PATI GARUT DENGAN SUBTITUSI PARSIAL TEPUNG JEWAWUT
Authors: Muhammad Dimas Rahadian Aji , Sasti Tabita Gita , Siswanti Siswanti , Anandito R. Baskara Katri
Published: July 2020

Tujuan penelitian ini adalah untuk mengetahui karakteristik sensoris, fisik, dan gizi brownies cokelat kukus berbahan dasar pati garut dengan subtitusi tepung jewawut (25%, 50%, 75%). Produk yang mempumyai nilai tertinggi dalam uji kesukaan konsumen adalah brownis cokelat kukus yang dibuat dari pati garut dan tepung jewawut dengan perbandingan 50:50. Oleh karena itu, sampel ini selanjutnya dipilih untuk digunakan dalam penelitian tahap selanjutnya. Sampel brownis cokela terpilih mempunyai kadar air 28,2%, kadar abu 1,8%, kadar protein 8,7%, kadar lemak 21,6%, kadar karbohidrat 77,1%, dan serat pangan 3,1%. Brownis cokelat kukus dengan subtitusi 50% tepung jewawut memberikan kontribusi sebesar 10% dari total kebutuhan sehari serat pangan golongan anak usia 5-6 tahun, sehingga dapat dipertimbangkan sebagai makanan sumber serat bagi kelompok konsumen anak-anak pada rentang usia tersebut.
La tragedia como motor narrativo de los videojuegos cinematográficos
Authors:   Universidad Rey Juan Carlos, CUADRADO ALVARADO ALFONSO
Published: July 2020

El cine y los videojuegos han mantenido de forma constante una relación ambivalente de rechazo, influencia y colaboración. Desde sus comienzos, la Ludología puso el centro de la experiencia del juego en su capacidad de ejercer el control y desarrollar amplia libertad en el mundo del juego. Esta visión ha hecho que se asimilara la ontología del videojuego con el tipo de actuaciones que fomentan libertad y control. Por el contrario, cualquier tipo de actuaciones vinculadas al placer estético de la narrativa han sido tildadas de opuestas a la esencia del juego y por ello propias de las narraciones o películas interactivas, manifestaciones siempre acompañadas de un tono peyorativo. Por ello es necesario realizar una revisión del estatuto ontológico del videojuego a través de la reformulación del significado de la actuación (agency). El artículo sigue los trabajos de Tanenbaum sobre la apertura del concepto de la actuación hacia la construcción de sentido y el compromiso con el personaje y la historia, y propone cómo el lenguaje cinematográfico y las fases esenciales de la tragedia aristotélica (compasión, temor y catarsis) son indispensables para entender el tipo de actuación de los videojuegos cinematográficos.
Experiencias del cine documental en México
Authors:   Universidad Miguel Hernández, Martínez Cano Francisco Julián
Published: July 2020

Cine Político en México (1968-2017) recoge la historia del cine mexicano fundamentalmente desde las experiencias de producciones documentales. Un género complejo de trabajar en el contexto social que se desarrolla, ya que mientras la ficción puede escudarse a la creatividad para desvelar o no momentos de la historia que hayan podido ser censurados, el género del documental no tiene esta posibilidad, sino que se espera realismo y veracidad. Complicado cuando la censura pendula sobre las cabezas de los autores, cuando existe el riesgo real de que la vida corre peligro. Si a esto se le añaden las dificultades propias de investigar en México (Jurado, 2010) en lo que tiene que ver con el acceso y conservación de algunos fondos documentales, resulta muy significativo el contenido que presente este libro. Los coordinadores de este número de la colección Transamerican Film and Literature, Adriana Estrada, Nicolas Défossé y Diego Zavala demuestran gran dominio del tema que tratan, como puede verse en los textos que firman, y una excelente elección en la selección de los capítulos que finalmente componen este volumen.
El poder de la experiencia y la cultura ludonarrativa. Marketing y videojuegos
Authors:   Universidad Miguel Hernández de Elche, Jurado Martín Montserrat
Published: July 2020

Llegamos al cierre del número de julio del volumen 11 de la revista situándonos en quartil 3 de Dialnet Metrics y trabajando duro para ofrecer a la comunidad de investigadores un producto de calidad que dé cobertura a contenidos en torno a la comunicación, con indidencia en los más especializados, y comprometido con la difusión de resultados de investigación en el ámbito de las ciencias sociales. La revista Miguel Hernández Communication Journal estrena imagen en una nueva década en un año que pasará a la historia por el confinamiento provocado por el COVID 2019. Presentamos aquí el número 11º como una auténtica joya de la investigación centrada en dos áreas: “nuevos marcos de reflexión y nuevos enfoques de investigación sobre la experiencia y las posibilidades que ofrece, en un amplio campo de disciplinas y actividades” (Martínez-Sala, 2020) recogido bajo el título El poder la experiencia; y sobre el videojuego, “convertido en una pieza fundamental del complejo ecosistema mediático contemporáneo, erigiéndose de forma innegable como una industria que, lejos de limitarse a satisfacer las nuevas demandas de ocio digital, posee un rol esencial en la configuración de la cultura del siglo XXI” (Hernández-Pérez y Albadalejo, 2020), en el monográfico titulado Cultura ludonarrativa: puntos de encuentro entre los medios tradicionales y los videojuegos
The quality of and women’s satisfaction with maternal referral practices in Sub-Saharan African low and lower-middle income countries: A systematic review
Authors: Ameyaw Edward Kwabena , Njue Carolyne , Tran Nguyen Toan , Dawson Angela
Published: July 2020

Background: Sub-Saharan African Low and Lower-Middle Income Countries (SSA LLMICs have the highest burden of maternal and neonatal morbidity and mortality in the world. Timely and appropriate obstetric referral to a suitable health facility is a sign of effective health system. This paper presents the findings of a systematic review that aimed to identify what referral practices are delivered according to accepted standards for pregnant women and newborns in SSA LLMICs by competent healthcare providers and in line with the need and wishes of women. Methods: Six electronic databases were systematically searched for primary data studies (2009-2018) in English reporting on maternal referral practices and their effectiveness. We conducted a content analysis guided by a framework for assessing the quality of maternal referral. The articles defined quality referral as: the timely identification of signal functions, established guidelines or standards, adequate documentation, staff accompaniment and prompt care by competent healthcare providers at the receiving facility. Results: Seventeen articles were included in the study. Most studies were quantitative (n=11). Two studies reported that women were dissatisfied due to delays in referral processes that affected their health. Most of the articles (10) reported that women were not accompanied to higher levels of healthcare, delays in referral processes, transportation challenges and poor referral documentation. Some healthcare providers administered essential drugs such as misoprostol prior to referral. Conclusions: Efforts to improve maternal health in LLMICs should aim to enhance maternal healthcare providers’ ability to identify conditions that demand referral. Low cost transport is needed to mitigate transportation barriers of referral. To ensure quality maternal referral, mechanisms should be instituted for health managers at the district level to monitor and evaluate referral documentation, quality and efficiency of maternal referrals on regular basis.
The state of child nutrition in Ethiopia: an umbrella review of systematic review and meta-analysis reports
Authors: Mohammed Shimels Hussien , Habtewold Tesfa Dejenie , Arero Amanuel Godana , Esmaillzadeh Ahmad
Published: July 2020

Background Malnutrition remains to be a major public health problem in developing countries, particularly among children under-5 years of age children who are more vulnerable to both macro and micro-nutrient deficiencies. Various systematic review and meta-analysis (SRM) studies were done on nutritional statuses of children in Ethiopia, but no summary of the findings was done on the topic. Thus, this umbrella review was done to summarize the evidence from SRM studies on the magnitude and determinants of malnutrition and poor feeding practices among under-5 children in Ethiopia. Methods PubMed, Embase, Scopus, Web of Sciences, Cochrane Database of Systematic Reviews, Database of Abstracts of Reviews of Effects, and Google Scholar were searched for SRM studies on magnitude and risk factors of malnutrition and child feeding practice indicators in Ethiopia. The methodological quality of the included studies was assessed using the Assessment of Multiple Systematic Reviews (AMSTAR) tool. The estimates of the included SRM studies on the prevalence and determinants of stunting, wasting, underweight, and poor child feeding practices were pooled and summarized with random-effects meta-analysis models. Result We included nine SRM studies, containing a total of 214,458 under-5 children from 255 observation studies. The summary estimates of prevalence of stunting, underweight, and wasting were 42% (95%CI=37-46%), 33% (95%CI=27-39%), and 15% (95%CI=12-19%), respectively. The proportion of children who met the recommendations for timely initiation of breastfeeding, exclusive breastfeeding during the first 6 months, and timely initiation of complementary feeding were 65%, 60%, and 62%, respectively. The proportion of children who met the recommendations for dietary diversity and meal frequency were 20%, and 56%, respectively. Only 10% of children fulfilled the minimum criteria of acceptable diet. There was a strong relationship between poor feeding practices and the state of malnutrition, and both conditions were related to various health, socio-economic, and environmental factors. Conclusion Child malnutrition and poor feeding practices are highly prevalent and of significant public health concern in Ethiopia. Only few children are getting proper complementary feeding. Multi-sectoral efforts are needed to improve children’s feeding practices and reduce the high burden of malnutrition in the country.
Psycho-Sexual Influence of Sterilization on Women’s Quality of Life: A Path Model
Authors: Youseflu Samaneh , Sadatmahalleh Shahideh Jahanian
Published: July 2020

Background: Tubal ligation (TL), as a permanent contraception method, have a negative and positive impact on women’s life. This study aimed to test a conceptual model considering the interrelated role of menorrhagia, body image concern, self-esteem, sexual function, anxiety and depression on quality of life (QOL) of sterilized women. Methods: The current study was conducted as a cross-sectional study on 200 sterilized women. Data were collected using a socio-demographic checklist, Short Form Health Survey (SF-12), Pictorial Blood Loss Assessment Chart (PBLAC), Female Sexual Function Index (FSFI), Hospital Anxiety and Depression Scale (HADS), Body Image Concern Inventory (BICI), and Rosenberg Self-esteem Scale. Data were analyzed using Pearson correlation coefficient and path analysis. Results: Results show that anxiety, sexual function, self-esteem, and body image dissatisfaction have a direct effect on women’s QOL. Higher level of anxiety, and body dissatisfaction directly reduce QOL. Sexual function, and menorrhagia, with an indirect effect through anxiety, reduces QOL. Higher level of anxiety with indirect effect thorough self-esteem can worsen QOL. Also, sexual function indirectly affects QOL through anxiety.Conclusion: It looks that the proposed predictors of this model are greatly important. These findings give support for a hypothetical model in which betterment in SF, body image satisfaction, self-esteem, anxiety, and menorrhagia led to a good QOL of sterilized women. Hence, in designing care for sterilized women, these factors should be considered.
Clinical Features and Prognosis of Gas-forming and Non-gas-forming Pyogenic Liver Abscess: A Comparative Study
Authors: Zhang Jia , Gao Yi , Du Zhaoqing , Ren Yifan , Bi Jianbin , Wu Zheng , Lv Yi , Wu Rongqian
Published: July 2020

The authors have withdrawn this preprint due to the presence of errors.
The Impact of Legalization of Access to Recreational Cannabis on Canadian Medical Users with Cancer.
Authors: Hawley Philippa Helen , Gobbo Monica , Afghari Narsis
Published: July 2020

Background Canada legalized cannabis use for medical purposes in 1999. Legalization of cannabis for recreational purposes in October 2018 offered the opportunity to assess the impact of recreational legalization on cancer patients’ patterns of use to identify learning points that could be of use to other countries considering similar legislation.Method Two identical anonymous cross-sectional surveys were administered to cancer patients in British Columbia(2 months before and 3 months following legalization), with the same eligibility criteria. The prevalence of medical cannabis use, the distribution of symptoms leading to use, the most common types of cannabis products and sources, reasons for stopping using cannabis, and barriers to access were assessed.Results The overall response rate was 27%. Both cohorts were similar regarding age (median= 66yrs), gender (53% of participants were female), and education (one-third of participants had an education level of high school or less). Respondents had multiple motives for taking cannabis, including to manage multiple symptoms, to treat cancer, and for recreational reasons. The majority of patients in both surveys did not use the legal medical access system, but sought products that have a low potential for euphoria, such as edibles. Comparison of the two cohorts showed that after legalization the prevalence of current cannabis use increased by 26% (23·1% to 29·1%, p-value 0·01), including an increased disclosure of recreational motive for use, from 32% to 40%. However, in the post-legalization cohort more Current Users reported problems getting cannabis (18%) than the pre-legalization cohort (8%), (p-value <0·01). The most common barrier cited was lack of available preferred products, including edibles, as these were only available from illegal dispensaries.Conclusions Results showed that legalization of cannabis for recreational purposes may have an impact on those who use medical cannabis. Impacts include an increase in prevalence of use; problems accessing preferred products legally; higher cost, and difficulties using a legal access system. The desired goal of regulation in reducing harms from use of illegal cannabis products are unlikely to be achieved if the legal process is less attractive to patients than use of illegal sources.
Impact of Multidisciplinary Tumour Boards (MTB) on the clinicopathological characteristics and outcomes of resected colorectal liver metastases across time
Authors: Chen Lionel , Syn Nicholas L. , Goh Brian K. P. , Cheow Peng Chung , Raj Prema , Koh Yexin , Chung Alexander , Lee Ser Yee , Ooi London Lucien , Chan Chung Yip , Teo Jin Yao
Published: July 2020

Background Resection of colorectal liver metastases (CLM) has been established as the standard of care. This study aims to compare the change in clinicopathological characteristics of patients who underwent curative resection of CLM across two time periods – 2000 to 2010 (P1) and 2011 to 2016 (P2) and evaluate the prognostic impact of these characteristics on survival outcomes. Methods Patients who undergo liver resection for CLM at Singapore General Hospital from January 2000 to December 2016 were identified from a prospectively maintained database. The primary end point was overall survival. Results There were 183/318 (57.5%) patients and 135/318 (42.5%) patients in P1 and P2 respectively. There was a lower proportion of patients who had nodal metastases from primary colorectal cancer and clinical risk score (CRS) less than 3 in P2 when compared to P1. There was no difference in survival between both time periods. Independent predictors of survival for the cohort were CEA levels ≥200 ng/ml, primary tumour grade and lymph nodal status. Independent predictors of poor survival in P1 were poorly differentiated colorectal cancer and nodal metastases while in P2, independent predictors of poor survival were multiple liver metastases and nodal metastases.Conclusion Nodal metastases from primary colorectal cancer is an independent predictor of poor survival across time for resectable CLM. Although there is no difference in survival between the two time periods, patients with multiple liver metastases should be carefully considered prior to surgery as it is also an independent predictor of overall survival.
Comparison of the effectiveness of Aloe Vera Gel with 2% Nitrofurazone ointment on the healing of superficial second-degree burns. Randomised clinical trial
Authors: Varaei Shokoh , ranjbar hadi , sabaghzadeh parichehr , bostani sanaz , amirsalari Soodabhe
Published: July 2020

Background: Burn injuries are one of the most common sources of trauma globally that comprise a significant drain on long-term personal and healthcare cost Large surface area burn wounds are difficult to manage and may result in significant physiologic and psychological sequelae. The aim of this study was to compare the effectiveness of Aloe Vera gel with 2%Nitrofurazone ointment in the healing of superficial partial thickness burns wounds. Methods: The present study was a split body controlled, randomized clinical trial. The sample was recruited from patients with superficial partial thickness burns wound who were prescribed to treat with 2% Nitrofurazone ointment. Thirty patients with at least two burn, each burn on an alternate side of the body, entered the study — samples allocated to two area which received Aloe Vera gel or 2% Nitrofurazone ointment on their burns. Bates-Jensen Wound. assessment tool (BWAT) was used to evaluate the healing of burns. the epithelialization parameter and sum score Bates-Jensen tools evaluated before, one, two and three weeks after the beginning of treatment. Results: The mean ± SD of epithelialization parameter in Aloe Vera area were 5.0±00.00, 4.0±46.57, 3.0±50.57, 2.0±56.62. The mean ± SD of epithelialization parameter in 2% Nitrofurazone ointment area were 5.0±00.00, 4.0±66.54, 3.0±76.50, 3.0±03.61.and The mean ± SD of BWAT scores in Aloe Vera area were 30.32 ± 3.28, 27.33 ± 3.38, 21.33 ± 3.13, 16.12 ± 2.16 respectively (F(2, 65.07) =440.00, p=0.001). The mean ± SD of BWAT scores in 2% Nitrofurazone ointment area were 30.51 ± 3.79, 28.45 ± 3.49, 23.36 ± 2.89, 19.23 ± 2.11 ( F(1, 52.00) =228.00, p=0.001).Conclusions: There is a significant difference in epithelialization parameter and (BWAT) scores between intervention and control area. Based on this study it looks like that aloe vera gel could promoted epithelialization and wound closure more effectively than 2% Nitrofurazone ointment
Transgelin interacts with PARP1 in human colon cancer cells
Authors: Lew Zhen-xian , Zhou Hui-min , Fang Yuan-yuan , Ye Zhen , Zhong Wa , Yang Xin-yi , Yu Zhong , Chen Dan-yu , Luo Si-min , Chen Li-fei , Lin Ying
Published: July 2020

Background: Transgelin, an actin-binding protein, is associated with cytoskeleton remodeling. Findings from our previous studies demonstrated that transgelin was up-regulated in node-positive colorectal cancer (CRC) versus node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcripts and increased the metastatic potential of colon cancer cells in vitro and in vivo. This study aims to explore the mechanisms through which transgelin participates in the metastasis of colon cancer cells.Methods: Immunofluorescence and immunoblotting analysis were used to determine the cellular localization of endogenous and exogenous transgelin in colon cancer cells. Co-immunoprecipitation and subsequently high-performance liquid chromatography/tandem mass spectrometry were performed to identify the proteins that were potentially interacting with transgelin. The 256 downstream transcripts regulated by transgelin were analyzed with bioinformatics methods to discriminate the specific key genes and signaling pathways. The Gene-Cloud of Biotechnology Information (GCBI) tools were used to predict the potential transcription factors (TFs) for the key genes. The predicted TFs corresponded to the proteins identified to interact with transgelin. The interaction between transgelin and the TFs was verified by co-immunoprecipitation and immunofluorescence.Results: Transgelin was found to localize in both the cytoplasm and nucleus of the colon cancer cells. Approximately 297 proteins were identified to interact with transgelin. The overexpression of TAGLN led to the differential expression of 184 downstream genes. Network topology analysis discriminated seven genes, including CALM1, MYO1F, NCKIPSD, PLK4, RAC1, WAS and WIPF1 , which are mostly involved in the Rho signaling pathway. Poly (ADP-ribose) polymerase-1 (PARP1) was predicted as the unique TF for the key genes and concurrently corresponded to the DNA-binding proteins potentially interacting with transgelin. The interaction between PARP1 and transgelin in human RKO colon cancer cells was further validated by immunoprecipitation and immunofluorescence assays. Conclusions: Our results suggest that transgelin binds to PARP1 and regulates the expression of downstream key genes, which are mainly involved in the Rho signaling pathway, and thus participates in the metastasis of colon cancer.
Circ_0007841 promotes the progression of multiple myeloma through targeting miR-338-3p/BRD4 signaling cascade
Authors: Wang Yan , Lin Quande , Song Chunge , Ma Ruojin , Li Xiaojie
Published: July 2020

Background: The pathogenesis of multiple myeloma (MM) is not completely known. Uncovering the potential mechanism of MM initiation and progression is essential for identifying novel diagnostic and therapeutic targets. Herein, we explored the function and the working mechanism of circular RNA circ_0007841 in MM progression. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression of circ_0007841, microRNA-338-3p (miR-338-3p) and bromodomain containing 4 (BRD4). Cell proliferation ability was analyzed through cell counting kit-8 (CCK8) assay, colony formation assay and flow cytometry. Transwell assays were conducted to measure the migration and invasion abilities of MM cells. Cell apoptosis was also assessed by flow cytometry. The interaction between miR-338-3p and circ_0007841 or BRD4 was confirmed by dual-luciferase reporter assay and RNA-pull down assay. Results: Circ_0007841 was highly expressed in bone marrow (BM)-derived plasma cells of MM patients and MM cell lines than that in healthy volunteers and normal plasma cell line nPCs. Circ_0007841 promoted the proliferation, cell cycle and metastasis and impeded the apoptosis of MM cells. miR-338-3p was a direct target of circ_0007841 in MM cells and circ_0007841 accelerated the progression of MM through targeting miR-338-3p. BRD4 could directly bind to miR-338-3p in MM cells and miR-338-3p exerted an anti-tumor role through targeting BRD4. Circ_0007841 promoted the activation of PI3K/AKT signaling via miR-338-3p/BRD4 axis. Exosomes generated from mesenchymal stromal cells (MSCs) elevated the malignant behaviors of MM cells via circ_0007841. Conclusion: Circ_0007841 acted as an oncogene to promote the proliferation, cell cycle and motility and restrain the apoptosis of MM cells through sequestering miR-338-3p to up-regulate the expression of BRD4.
C6-ceramide treatment inhibits the proangiogenic activity of multiple myeloma exosomes via the miR-29b/Akt pathway
Authors: Liu Liping , Ye Qinmao , Liu Langni , Bihl Ji Chen , Chen Yanfang , Liu Jing , Cheng Qian
Published: July 2020

Background: The increased bone marrow angiogenesis is involved in the progression of multiple myeloma (MM) with the underlying mechanism poorly understood. Cancer-released exosomes could play an important role in the pathological angiogenesis through exosomal microRNAs (miRs) delivery. MiR-29b has been reported in regulating the tumor angiogenesis. Methods: In this study, we explored the role of C6-ceramide (C6-cer, a Ceramide pathway activator) in the angiogenic effect of MM exosomes and its potential mechanism. MM cells (OPM2 and RPMI-8226) treated with C6-cer were studied for its effects on the endothelial cell (EC) functions . Results: Our results showed that exosomes released from MM cells treated by C6-cer ( Exo C6-cer ) significantly inhibited the proliferation, migration and tube formation of ECs. For mechanism studies, we found that the level of miR-29b was increased in ECs treated by exo C6-cer , while mRNA and protein expressions of Akt3, PI3K and VEGFA were decreased in ECs, indicating the involvement of Akt pathway. Furthermore, downregulation of miR-29b by inhibitor administration could prevent the exo C6-cer -induced cell proliferation, migration and angiogenesis of ECs, accompanied with the increased expressions of Akt3, PI3K and VEGFA. Conclusions: Collectively, our data suggest that exo C6-cer -mediated miR-29b expression participates in the progression of MM through suppressing the proliferation, migration and angiogenesis of ECs by targeting Akt signal pathway.
Intralipid postconditioning in patients of Cardiac surgery undergoing cardioPulmonary Bypass (iCPB): study protocol for a randomized controlled trial
Authors: Yuan Yuan , Xiong Hui , Zhang Yan , Yu Hong , Zhou Rong-Hua
Published: July 2020

Background: Intralipid is a necessary fatty acid carrier that has been safely used as an energy supplier in the clinic. It has played an important role in rescuing the cardiac arrest caused by local anesthetic toxicity. In recent years, experimental studies have shown that intralipid postconditioning (ILPC) could reduce myocardial ischemic/reperfusion (I/R) injuries. Our research group has innovatively conducted a pilot randomized controlled trial (RCT) and the results showed that ILPC could reduce the release of cTnT and CK-MB, biomarkers of myocardial I/R injury, in valve replacement surgery. However, the potential effects of ILPC on the clinical outcome of adult cardiac surgery patients are unclear. Intralipid postconditioning in patients of cardiac surgery undergoing cardiopulmonary bypass (iCPB) trial is aimed to further study whether ILPC could improve short-term and long-term clinical outcome, as well as cardiac function in adult cardiac surgery patients.Methods: The iCPB trial is an ongoing, single-center, prospective, double blinded, large sample RCT. In total, 1000 adults undergoing cardiac surgery will be randomly allocated to either ILPC group or the control group. Intervention group received an intravenous infusion of 2 mL/kg of 20% intralipid (medium-chain and long-chain fat emulsion injection C6~C24, Pharmaceutical) within 10 minutes before aortic cross-unclamping, and the control group received an equivalent volume of normal saline. The primary endpoints are complex morbidity of major complications during hospitalization, and all-cause mortality within 30 days after surgery. The secondary endpoints include: (1) all-cause mortality 6 months and 1 year postoperatively, (2) the quality of life within 1 year after surgery，using QoR-15 questionnaire, (3) the postoperative cardiac function evaluated by LVEF, LVEDs, LVEDD, and the myocardial injury evaluated by CK-MB, cTnT, BNP, (4) short-term clinical outcomes during hospitalization and total cost are also detailed evaluated. Discussion: The iCPB trial is the first to explore ILPC on the clinical outcome of adult cardiac surgery patients. The results are expected to provide potential evidences about whether ILPC could reduce the morbidity and mortality, improve the cardiac function and quality of life. Therefore, provide a rationale for the evaluation of the potentially clinically relevant benefit of intralipid therapy.Trial registration: Chictr.org.cn, ChiCTR1900024387. Prospectively registered on 9 July 2019.
Sagittal Spinal-pelvic Alignment in Patients with Crowe Type IV Developmental Dysplasia of the Hip
Authors: Ren Peng , Kong Xiangpeng , Chai Wei , Wang Yan
Published: July 2020

Background: The impact of high dislocated dysplastic hips on spinal-pelvic alignment has not been well described. This study aims to evaluate compensatory spinal radiographic changes and presence of back pain in patients with Crowe type IV developmental dysplasia of the hip (DDH).Methods: An observational study was conducted from July 2016 to December 2017, and 49 consecutive patients with Crowe IV DDH were enrolled. Forty-nine sex- and age-matched asymptomatic healthy adults were recruited as the controls. The sacral slope (SS), lumbar lordosis (LL), spino-sacral angle (SSA), C7 tilt (C7T), and sagittal vertical axis (SVA[C7]) were measured on lateral whole spine radiographs. The presence of low back pain (LBP) and visual analogue scale (VAS) scores were recorded. Results: The patients with Crowe IV DDH showed significantly greater SS (47.5±7.5° vs. 40.4±6.7°, p<0.05), LL (-63.7±9.2° vs. -53.3±11.5°, P<0.05), SSA (141.8°±7.2° vs. 130.6±7.9°, p<0.05), C7T (93.9±3.6° vs. 91.1±3.7°, P<0.05), and lower SVA(C7) (-16mm[-95-45] vs. 6.4mm[-52-47], p<0.05) compared to the controls. The patients with bilateral Crowe IV DDH also exhibited larger SS, LL, SSA, and C7T and a smaller SVA (C7) than those with unilateral Crowe IV DDH. Sixty-three percent of the patients with Crowe IV DDH reported LBP.Conclusion: The patients with Crowe IV DDH exhibited abnormal spinal-pelvic alignment characterized by anterior pelvic tilt, lumbar hyperlordosis, and a backward-leaning trunk. Bilateral Crowe IV DDH had a greater impact on spinal-pelvic alignment than unilateral Crowe IV DDH.
A description of the influenza vaccination program for health care workers in Vietnam
Authors: Ha Nga , Nguyen Thoa Thi Minh , Nguyen Tung Xuan , Tran Phu Dac , Nguyen Hang Minh , Ha Van Thi Cam , Lafond Kathryn E. , Seward Jane F. , McFarland Jeffrey W. , Chu Susan Y.
Published: July 2020

Background: In 2017, the Vietnam Ministry of Health conducted a demonstration project to introduce seasonal influenza vaccination to health care workers. A total of 11,000 doses of influenza vaccine, single-dose prefilled syringes, were provided free to HCWs at 29 selected hospitals, clinics, and research institutes in four provinces: Hanoi, Khanh Hoa, Dak Lak and Ho Chi Minh City. Methods: Before the campaign, a workshop was organized to discuss an implementation plan including technical requirements, cold chain, uptake reporting, and surveillance for adverse events following immunization. All sites distributed communication materials and encouraged their staff to register for vaccination. Following immunization sessions, sites sent reports on uptake and adverse events following immunization. Left-over vaccine was transferred to other sites to maximize vaccine use. Results: The average uptake was 57% for all health care workers, with 11 sites achieving 90% and above. These 11 sites were small with less than 500 staff, including 5 primary hospitals, 3 preventive medicine units, and 2 referral hospitals. Among the six biggest sites with over 1,000 staff, four sites had the lowest uptake (14%-47%). Most of the high-uptake sites were from the central to the south; only one site, a referral hospital, was from the north. After redistribution of left-over vaccine, only 130 vaccine doses (1.2%) were not used and destroyed. Based on factors that affected uptake, including registration levels, differing communication strategies, availability of vaccination, and commitment by health facility leaders, we recommended ways to increase health care worker coverage; recommendations to improve reporting adverse events following immunization were also made.Conclusions: The project demonstrated that it was feasible to conduct influenza vaccination campaigns among health care workers in Vietnam. Improvements in promotion of registration, more intense pre-planning, especially at larger facilities, and wider, more consistent availability of communication materials will result in increased efficiency and coverage in this program’s future expansion.
LncRNA GAS5 suppresses the proliferation and invasion of osteosarcoma cells via the miR-23a-3p/PTEN/PI3K/AKT pathway
Authors: Liu Jianmin , Chen Ming , Ma Longyang , Dang Xingbo , Du Gongliang
Published: July 2020

Background: Accumulating evidence has shown that lncRNA growth arrest special 5 (GAS5) is a well‑known tumor suppressor in the pathogenesis of a variety of human cancers. However, the detailed role of GAS5 in osteosarcoma is largely unclear. Here, we explore the role of GAS5 in progression of osteosarcoma. Methods: The expression level of GAS5 was detected in human osteosarcoma tissues and matched adjacent tissues, as well as osteosarcoma cell lines and non-malignant osteoblast cells. Then, in vitro gain- and loss-of-function experiments, with the pcDNA-GAS5 expression vector and GAS5-siRNA, were performed in U2OS and HOS cells to determine the effect of GAS5 on osteosarcoma cell proliferation and invasion. Subsequently, we searched potential miRNA targets with bioinformatics analysis and confirmed their interaction by using luciferase reporter gene and RNA pull-down assays. The function and mechanism of miR-23a-3p in proliferation and invasion was also investigated in U2OS and HOS cells. Furthermore, rescue experiments were performed to verify the involvement of miR-23a-3p and its target gene in GAS5-mediated cell behaviors. Finally, a xenograft nude mouse model was established by subcutaneous injection with U2OS cells overexpressing GAS5 or not, and the effect of GAS5 on tumor growth in vivo was evaluated. Results: GAS5 was downregulated in human osteosarcoma tissues and cell lines. Overexpression of GAS5 could significantly suppress, and downregulation of GAS5 promoted, proliferation and invasion of osteosarcoma cells. GAS5 could directly bind with and downregulated miR-23a-3p that post-transcriptionally downregulated the tumor suppressor PTEN and positively regulated proliferation and invasion of osteosarcoma cells. Rescue experiments confirmed the involvement of miR-23a-3p and PTEN in GAS5-mediated cell behaviors by modifying the phosphatidylinositol-3-kinases/protein-serine-threonine kinase (PI3K/AKT) pathway. GAS5 could inhibit tumor growth in vivo . Conclusion: GAS5 functions as a competing endogenous RNA , sponging miR-23a-3p, to promote PTEN expression and suppress cell growth and invasion in osteosarcoma by regulating the PI3K/AKT pathway.
Grit protects medical students from burnout: a longitudinal study
Authors: Jumat Raihan , Chow Pierce Kah-Hoe , Allen John , Lai Siang-Hui , Hwang Nian-Chih , Iqbal Jabed , Mok May Un Sam , Rapisarda Attilio , Velkey John Matthew , Engle Deborah , Compton Scott
Published: July 2020

Background Burnout is a serious issue plaguing the medical profession with potential negative consequences on patient care. Burnout symptoms are observed as early as medical school. Based on a Job Demands-Resources model, this study aims to assess associations between specific job resources measured at the beginning of the first year of medical school with burnout symptoms occurring later in the first year. Methods The specific job resources of grit, tolerance for ambiguity, social support and gender were measured in Duke-NUS Medical School students at the start of Year 1. Students were then surveyed for burnout symptoms at approximately quarterly intervals throughout the year. Using high ratings of cynicism and exhaustion as the definition of burnout, we investigated the associations of the occurrence of burnout with student job resources using multivariable logistic regression analysis. Results Out of 59 students, 19 (32.2%) indicated evidence of burnout at some point across the first year of medical school. Stepwise multivariable logistic regression analysis identified grit as having a significant protective effect against experiencing burnout (Odds Ratio, 0.84; 95%CI 0.74 to 0.96). Using grit as a single predictor of burnout, area under the ROC curve was 0.76 (95%CI: 0.62 to 0.89). Conclusions Grit was identified as a protective factor against later burnout, suggesting that less gritty students are more susceptible to burnout. The results indicate that grit is a robust character trait which can prognosticate burnout in medical students. These students would potentially benefit from enhanced efforts to develop grit as a personal job resource.