bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Subgroups of Children in the Australian Autism Biobank using Latent Class Analysis
Authors: Montgomery Alicia , Masi Anne , Whitehouse Andrew , Veenstra-VanderWeele Jeremy , Shuffrey Lauren , Shen Mark , Karlov Lisa , Uljarevic Mirko , Alvares Gail , Woolfenden Susan , Silove Natalie , Eapen Valsamma
Published: August 2022

Background: The identification of reproducible subtypes within autistic populations is a priority research area in the context of neurodevelopment, to pave the way for identification of biomarkers and targeted treatment recommendations. Few previous studies have considered medical comorbidity alongside behavioural, cognitive, and psychiatric data in subgrouping analyses. This study sought to determine whether differing behavioural, cognitive, medical, and psychiatric profiles could be used to distinguish subgroups of children on the autism spectrum in the Australian Autism Biobank (AAB).Methods: Latent profile analysis was used to identify subgroups of children on the autism spectrum within the AAB (n=1151), utilising data on social communication profiles and restricted, repetitive, and stereotyped behaviours (RRBs), in addition to their cognitive, medical, and psychiatric profiles.Results: Our study identified four subgroups of children on the autism spectrum with differing profiles of autism traits and associated comorbidities. Two subgroups had more severe clinical and cognitive phenotype, suggesting higher support needs. For the ‘Higher Support Needs with Prominent Language and Cognitive Challenges’ subgroup, social communication, language and cognitive challenges were prominent, with prominent sensory seeking behaviours. The ‘Higher Support Needs with Prominent Medical and Psychiatric and Comorbidity’ subgroup had the highest mean scores of challenges relating to social communication and RRBs, with the highest probability of medical and psychiatric comorbidity, and cognitive scores similar to the overall group mean. Individuals within the ‘Moderate Support Needs with Emotional Challenges’ subgroup, had moderate mean scores of core traits of autism, and the highest probability of depression and/or suicidality. A fourth subgroup contained individuals with fewer challenges across domains (the ‘Fewer Support Needs Group’).Limitations: Data utilised to identify subgroups within this study was cross-sectional as longitudinal data was not available.Conclusions: Our findings support the holistic appraisal of support needs for children on the autism spectrum, with assessment of the impact of co-occurring medical and psychiatric conditions in addition to core autism traits, adaptive functioning, and cognitive functioning. Replication of our analysis in other cohorts of children on the autism spectrum is warranted, to assess whether the subgroup structure we identified is applicable in a broader context beyond our specific dataset.
RETRACTED: A wireless body area network enabled machine learning-assisted biosensor framework for detecting and analyzing ventilator and hospital-acquired pneumonia using the internet of things
Authors: M Abubeker K , Baskar S
Published: August 2022

The authors have requested that this preprint be removed from Research Square.
Synthesis of Mo–doped symbiotic mixture of Bi2WO6 and Bi6O6(OH)3(NO3)3·1.5H2O nanosheets with enhanced photocatalytic degradation for Mustard gas simulator 2-CEES
Authors: Yang Haoguo , Guo Yijun , Han Shitong , Xi Hailing , Lin Huaxiang , Yuan Rusheng , Long Jinlin , Lin Qun
Published: August 2022

Symbiotic mixture of Bi2WO6 and Bi6O6(OH)3(NO3)3·1.5H2O nanosheets doped with Mo (Mo-B-B) have been synthesized by a facile hydrothermal treatment. The as-prepared samples were investigated by XRD, DRS, XPS, SEM, TEM, Raman and PL. The photocatalytic activity of the samples were test by the degradation of 2-CEES under visible-light irradiation. The results showed that the doped Mo reduce the Eg of bare symbiotic mixture and improve the photocatalytic activity. The optimum amount of doped Mo is 5% that exhibit the best activity for degradation for 2-CEES. The FT-IR test confirmed that the degradation products of 2- CEES are mainly CO2 and CO.
Dispersal in Time-
the role of seed banks in patchy population dynamics of an aquatic plant
Authors: Tomowski Maxi , Lozada-Gobilard Sissi , Jeltsch Florian , Tiedemann Ralph
Published: August 2022

Progressive habitat fragmentation increasingly threatens plant species with narrow habitat requirements. While local environmental conditions largely define population growth rates and recruitment success at the patch level, dispersal is critical for population viability at the landscape scale. However, identifying dynamics of plant metapopulations in habitat networks is often confounded by the uncertainty of the extent of dormant population compartments. Here, we combined a landscape-scale assessment of an amphiphytic species’ population structure with detailed measurements of dispersal complexity in time to reveal past and present dispersal events and putative shifts in functional connectivity. Using 13 polymorphic microsatellite markers, we analyzed the genetic structure of extant Oenanthe aquatica populations and their soil seed banks in a kettle hole system to uncover hidden connectivity among subpopulations in both time and space. Considerable spatial genetic structure and isolation-by-distance (IBD) patterns suggest limited gene flow between population sites. In contrast, patch level measures of isolation and habitat availability showed minor effects on genetic diversity levels. Low differentiation along the time gradient highlights the superior role of temporal over spatial dispersal. Though some instances of recent colonisation were observed, our results suggest the actual genetic structure of kettle hole populations to result from multiple local seed rain events. Our findings uncover stepping-stone dynamics with source-sink effects based primarily on dispersal from long-term-persistent local populations to adjacent populations. Overall, spatio-temporal connectivity patterns provide support for metapopulation dynamics in our studied system and highlight the importance of persistent seed banks as long-term source of genetic diversity.
Radiation-induced sarcomas of the breast: A review of a 20-year single-centre experience
Authors: Lalla Vanessa Di , Tolba Marwan , Khosrow-Khavar Farzin , Baig Ayesha , Freeman Carolyn , Panet-Raymond Valerie
Published: August 2022

Purpose
Radiation-induced sarcomas (RIS) are histologically proven sarcomas within or around a previously irradiated site, per Cahan’s criteria. RIS incidence is higher in breast cancer compared to other solid cancers and prognosis remains poor given limited treatment options. This study aimed to review 20-year experience with RIS at a large tertiary care centre.
Methods
Using our institutional cancer registry database, we included patients meeting Cahan’s criteria diagnosed between years 2000 to 2020. Patient demographics, oncologic treatment, and oncologic outcomes data were collected. Descriptive statistics were used to describe demographic data. Oncologic outcomes were assessed using the Kaplan Meier method.
Results
Nineteen patients were identified. The median age at RIS diagnosis was 72 years (range 39–82) and median latency period for development of RIS was 112 months (range 53–300). All patients underwent surgery, 3 patients received systemic therapy, and 6 patients received re-irradiation as salvage treatment. The median follow-up time was 31 months (range 6-172) from diagnosis of RIS. Overall, 5 patients had local recurrence and 1 patient developed distant metastases. The median time to progression was 7 months (range 4–14). The progression-free survival (95% CI) at two years was 56.1% (37.4–84.4%). At two years follow-up after sarcoma diagnosis, the overall survival (95% CI) was 88.9% (75.5–100%).
Conclusion
While breast RIS remains rare, when managed in a high patient-volume centre, overall survival outcomes appear favorable. A significant proportion of patients recur locally after maximal treatment and require salvage therapy to improve outcome. These patients should be managed in high-volume centres where multidisciplinary expertise is available.
SMPD1 Expression Profile and Mutation Landscape Help Decipher Genotype–Phenotype Association and Precision Diagnosis for Niemann–Pick Disease Types A and B
Authors: Wang Ruisong , Qin Ziyi , Huang Long , Luo Huiling , Peng Han , Zhou Xinyu , Zhao Zhixiang , Liu Mingyao , Yang Pinhong , Shi Tieliu
Published: August 2022

Background: Types A and B of the rare genetic disease Niemann–Pick disease (NPD) are caused by mutations in the SMPD1 gene, which encodes sphingomyelin phosphodiesterase (ASM). Except for the liver and spleen enlargement and lung disease, the two subtypes have different onset times, survival times, ASM activities, and neurological abnormalities. To comprehensively explore the genotype-phenotype association and pathophysiological characteristics of NPD, we collected 144 NPD cases with strict quality control through literature mining. Results: The difference in ASM activity can differentiate NPD type A from other subtypes, with the ratio of ASM activity to the reference values being lower in type A (threshold 0.045 (4.45%)). Severe variations, such as deletion and insertion, can cause complete loss of ASM function, leading to type A, whereas relatively mild missense mutations generally result in type B. Among reported mutations, p.Arg3AlafsX76 mutation is highly prevalent in the Chinese population, and p.R608del mutation is common in Mediterranean countries. The expression profiles of SMPD1 from GTEx and single-cell RNA sequencing data of multiple fetal tissues showed that high expressions of SMPD1 can be observed in the liver, spleen, and brain tissues of adults and in hepatoblasts, hematopoietic stem cells, STC2_TLX1-positive cells, mesothelial cells of the spleen, vascular endothelial cells of the cerebellum and the cerebrum of fetuses, indicating that SMPD1 dysfunction is highly likely to have a significant effect on the function of those cell types during development and the clinicians need pay attention to these organs or tissues as well during diagnosis. In addition, we also predicted 21 new pathogenic mutations in the SMPD1 gene that potentially cause the NPD, signifying that more rare cases will be detected with those mutations in SMPD1. Conclusions: Our study is the first one to elucidate the effects of SMPD1 mutation on cell types and at the tissue level, which provides new insights into the genotype-phenotype association and can help in the precise diagnosis of NPD.
Simulation and Monitoring of Marine Diesel engine Machining workshop based on digital twin
Authors: Fang Xifeng , Guo Mingyi , Hu Zhongtai , Wu Qi , Zhang Shengwen , Gong Chanyuan , Wang Tongyue , Li Qun
Published: August 2022

In order to solve the current large processing workshop information degree is low, part of the information can not be immediately grasped, reaction lag and other problems. In this paper, key techniques of twin Marine diesel engine to develop digital processing workshops, the traditional Marine diesel engine processing workshop combined with digital twin technology, through the twin body build four-dimensional model the virtual workshop is established in this paper, based on the digital twin software service platform building workshop, unicom virtual workshop and physical workshop, make full use of the whole life cycle of Marine diesel engine processing data, Realize twin simulation and monitoring of physical workshop. Finally, the practical case is used to verify the practicality of the system.
In God’s virtual shoes: embodying the avatar of an omnipotent agent modulates the perception of one’s own abilities and enhances feelings of invulnerability
Authors: Frisanco Althea , Schepisi Michael , Tieri Gaetano , Aglioti Salvatore Maria
Published: August 2022

Immersive virtual reality can give people the illusion of owning artificial bodies (i.e., avatars) and controlling their actions. Tellingly, people appear to adhere to the newly embodied entities not just on the basis of physical traits but also behaving accordingly with the hallmarks of the represented characters. In two studies we pushed the limits of this process by testing if one’s own sense of power could be affected by embodying the anthropomorphic representation of the Christian God, that is considered an omnipotent entity. A human Muscled and a Normotype avatar were used as controls. Results showed that participants embodying the God-avatar: i) reacted to a threatening event compromising their physical safety by exhibiting a lower skin conductance response and heart rate deceleration compared to the Normotype-control avatar (Study 1); ii estimated they had more physical abilities compared to both the Normotype-control and the Muscled-control avatars (Study 2). Taken together, our findings suggest that embodying an omnipotent agent may exert an influence on people’s perception of their own limits and capabilities, nourishing feelings of physical invulnerability and strength. Our study indicates that effectively embodying virtual role models may boost achievements and have translational implications in the field of empowerment.
Changes in Self-Reported and Accelerometer-Measured Physical Activity Among Pregnant TRICARE Beneficiaries
Authors: Pebley Kinsey , Farage Gregory , Hare Marion E. , Bursac Zoran , Andres Aline , Chowdhury Sultana Mubarika Rahman , Talcott Gerald Wayne , Krukowski Rebecca A.
Published: August 2022

Background:Physical activity is recommended for all pregnant individuals and can prevent excessive gestational weight gain. However, physical activity has not been assessed among military personnel and other TRICARE beneficiaries, who experience unique military lifestyles. The current study assessed physical activity among pregnant TRICARE beneficiaries, both active duty and non-active duty, as measured by accelerometry and self-report data to examine potential predictors of physical activity engagement in the third trimester, and if self-report data was consistent with accelerometry data. We expected having a lower BMI, being active-duty, and having higher baseline physical activity engagement to be associated with higher physical activity at 32-weeks. We also hypothesized that accelerometry data would show lower physical activity levels than the self-reported measure.Methods:Participants were 430 TRICARE adult beneficiaries (204 Active Duty; 226 non-Active Duty) in San Antonio, TX who were part of a randomized controlled parent study that implemented a stepped-care behavioral intervention. Participants were recruited if they were less than 12-weeks gestation and did not have health conditions precluding dietary or physical activity changes (e.g., uncontrolled cardiovascular conditions) or would contribute to weight changes. Participants completed self-report measures and wore an Actical Activity Monitor accelerometer on their wrist to collect physical activity data at baseline and 32-weeks gestation.Results:Based on the accelerometer data, 99% of participants were meeting moderate physical activity guidelines recommending 150 minutes of moderate activity per week at baseline, and 96% were meeting this recommendation at 32-weeks. Based on self-report data, 88% of participants at baseline and 92% at 32-weeks met moderate physical activity recommendations. Linear regression and zero-inflated negative binomial models indicated that baseline physical activity engagement predicted moderate physical activity later in pregnancy above and beyond BMI and military status. Surprisingly, self-reported data, but not accelerometer data, showed that higher baseline activity was associated with decreased vigorous activity at 32-weeks gestation. Additionally, self-report and accelerometry data had small correlations at baseline, but not at 32-weeks.Conclusions:Future intervention efforts may benefit from intervening with individuals with lower pre-pregnancy activity levels, as those who are active seem to continue this habit.
DEVELOPMENTAL EFFECTS OF BISPHENOL A and ITS ANOLOGS BISPHENOL S, BISPHENOL F AND BISPHENOL AF ON EMBRYONIC DEVELOPMENT OF SEA URCHINS Paracentrotus lividus (Lamarck 1816) and Arbacia lixula (Linnaeus 1758)
Authors: Arslan Özlem Çakal
Published: August 2022

Restricting the use of BPA, environmental concentrations of Bisphenol S, bisphenol F and bisphenol AF begin to increase. Because of, no data is available about the possible toxic effects of BPA analogs on sea urchin embryos, this study aims to investigate the developmental effects of bisphenol and its analogs on sea urchin embryos by embryotoxicity bioassay with two sea urchin species Paracentrotus lividus and Arbacia lixula. The EC50 (Effective Concentration 50) for 72 hours was determined as 1.396 mg-BPA/L for sea urchin A. lixula and 0.676 mg-BPA/L (EC50) was estimated for BPA for P. lividus. The EC50 for BPS, BPF, and BPAF were determined as 2,673 mg-BPS/L, 1,129 mg-BPF/L, and 0,314 mg-BPAF/L for A. lixula. It can be concluded that these chemicals adversely affect the embryonic developmental stages of the P. lividus and A. lixula this is of great ecological importance due to the hazard at the population level.In this way, result of this study present the nominal effective concentrations of BPA and its analogs and the suitability of the species for use as a biomarker in ecotoxicology tests.
Young serum protects against memory impairment in APP/PS1 transgenic mice by blocking neutrophil infiltration
Authors: Guo Kaihua , Qi Fangfang , Zuo Zejie , Hu Kaishun , Wang Rui , Wu Tong , Liu Hao , Tang Jiaoling , Wang Qingbo , Xie Yufeng , Tan Liren , Yang Yunjie , Zhang Xiaoran , Xu Jie , Yao Zhibin , Wang Shengwen , Wu Long-Jun
Published: August 2022

Activation of innate immunity in the brain is a prominent feature of Alzheimer's disease (AD). The present study investigated the regulation of innate immunity by young serum in a transgenic AD mouse model. We found that young serum significantly reduced the number of neutrophils and microglial reactivity in the brains of APP/PS1 mice. Neutrophil depletion via Ly6G neutralizing antibodies mimicked the benefits of young serum on AD brain functions. Serum proteomic analysis of young serum revealed significant enrichment of the factors VEGF-A and CXCL1, which are crucial for neutrophil migration and chemotaxis, leukocyte migration, and cell chemotaxis. Intravenously injected VEGF-A reversed Aβ-induced decreases in Cdk5 and increases in CXCL1 in vitro and blocked neutrophil infiltration into the AD brain. Endothelial Cdk5 overexpression conferred an inhibitory effect on CXCL1 and neutrophil infiltration and thereby restored memory in APP/PS1 mice. Our data uncover a previously unknown link between blood-derived VEGF signaling and neutrophil infiltration and provide a rationale for targeting endothelial Cdk5 signaling as a potential therapeutic strategy for AD.
Ecological speciation of Japanese Hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy
Authors: Sugawara Ryo , Aoki Wataru , Yamada Akiyoshi , Nakagiri Akira , Endo Naoki
Published: August 2022

Hydnum repando-orientale is an East Asian species closely related to H. boreorepandum and H. repandum; all three species produce edible mushrooms. We identified two ecological groups of H. repando-orientale in Japan: a temperate group occurring in Fagaceae-dominated forest at < 1200 m a.s.l. (ROF), and a subalpine group occurring in coniferous forest in highland at > 1900 m a.s.l. (ROC). We re-examined the taxonomy of the two ecological groups of H. repando-orientale using integrative approaches. Phylogenies of the two ecological groups and other related species were inferred from the internal transcribed spacer (ITS) and gene portions encoding the large subunit of nc rRNA (LSU), translation elongation factor-1 alpha (TEF1), RNA polymerase II largest subunit (RPB1), and RNA polymerase II second-largest subunit (RPB2). The concatenated phylogenetic tree separated the two ecological groups into well-supported sister clades. Also, species delimitations based on the topological congruence (GCPSR) and multispecies coalescent model (GMYC and BP&P) supported to separate the two ecological groups. Morphological analysis showed that ROC specimens had significantly larger basidiospores, compared with ROF specimens. Mon-mon mating tests using six ROF, three ROC, and three H. boreorepandum strains each showed independent incompatible groups, whereas one ROC strain showed compatibility with both ROC and ROF populations. Based on these results, we defined the ROC group as a new species, H. subalpinum. Because H. repando-orientale and H. subalpinum have smaller genetic divergence in nc rDNA and maintain slight sexual compatibility, they may have recently speciated in East Asia.
Towards Hybrid Collaboration Spaces - A Model of Support Requirements for Hybrid Collaboration
Authors: Augstein Mirjam , Neumayr Thomas , Schönböck Johannes , Kovacs Carrie
Published: August 2022

Triggered by the COVID-19 pandemic, the popularity of hybrid work and collaboration has increased drastically in recent years. This raises a need for diverse and flexible Hybrid Collaboration Spaces (i.e., the physical and virtual rooms in which hybrid collaborative interactions happen) tailored to the respective settings. So far, to the best of our knowledge, no general formal elicitation of requirements for such spaces has taken place. The elicitation and definition of (user or system) requirements is often a lengthy and costly process. This is even more true for novel settings which lack an established catalog of lessons learned. Moreover, requirements established for one scenario might be poorly generalizable to other scenarios. In this conceptual work, we first systematically compare existing CSCW models and frameworks, and then propose a novel two-view model of requirements for hybrid meetings and collaboration spaces. Our model builds upon and joins the descriptive power of two existing instruments: the Domino framework and its hybrid collaborative coupling styles of Neumayr et al., and Bill Buxton's Media Spaces (i.e., Person, Task, and Reference Space). It is intended to allow for the a priori estimation of which support mechanisms might be beneficial in a certain hybrid collaboration scenario.
Acid Ceramidase Gene Therapy Ameliorates Pulmonary Arterial Hypertension with Right Heart Dysfunction
Authors: Katz Michael G. , Hadas Yoav , Vincek Adam , Freage-Kahn Lina , Shtraizent Nataly , Madjarov Jeko M. , Pastuszko Peter , Eliyahu Efrat
Published: August 2022

BackgroundUp-regulation of ceramides in pulmonary arterial hypertension (PAH), contributing to perturbations in sphingolipid homeostasis and the transition of cells to a senescence state. We assessed the safety, feasibility, and efficiency of acid ceramidase gene transfer in a rodent PAH model.MethodsA model of PAH was created by the combination of pneumonectomy and injection of Sugen toxin. Magnetic resonance imaging and right heart catheterization confirmed development of PAH. Animals were subjected to intratracheal administration of synthetic adeno-associated viral vector (Anc80L65) carrying the acid ceramidase (Anc80L65.AC), an empty capsid vector, or saline. Therapeutic efficacy was evaluated 8 weeks after gene delivery.ResultsHemodynamic assessment four weeks after PAH model creation demonstrated an increase in the mean pulmonary artery pressure to 30.4 ± 2.13 mmHg versus 10.4 ± 1.65 mmHg in sham (p < 0.001), which was consistent with the definition of PAH. We documented a significant increase in pulmonary vascular resistance in the saline-treated (6.79 ± 0.85 mm Hg) and empty capsid (6.94 ± 0.47 mm Hg) groups, but not in animals receiving Anc80L65.AC (4.44 ± 0.71 mm Hg, p < 0.001). Morphometric analysis demonstrated an increase in medial wall thickness in control groups in comparison to those treated with acid ceramidase. After acid ceramidase gene delivery, a significant decrease of pro-inflammatory factors, interleukins, and senescence markers was observed.ConclusionGene delivery of acid ceramidase provided tropism to pulmonary tissue and ameliorated vascular remodeling with right ventricular dysfunction in pulmonary arterial hypertension.
Construction an autophagy-clinical prognostic index in oral cancer
Authors: Jiang Jirui , Zhen Zhanglong , Yin Junhao , Zeng Xiaoli , Shan ZhengNan , Li Shengjiao , Li Jia
Published: August 2022

Background: Autophagy, is a metabolic pathway that occurs in eukaryotic cells and regulated by autophagy-related genes (ARGs).The occurrence and development of many diseases are caused by abnormal autophagy. The purpose of this article is to explore the relationship between autophagy and prognosis of oral cancer, hoping to provide a new way for early diagnosis and guide doctors to make subsequent treatment decisions.Methods: Download the RNA seq and clinical features of 305 oral cancer and 30 non-tumor patients from The Cancer Genome Atlas (TCGA) dataset. Filtered out differential expression autophagy-related genes (ARGs),and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed these ARGs. Cox regression analysis filtered out the prognostic ARGs and constructed a risk score models for overall survival (OS) .Divided patients into high-risk and low-risk groups based on median risk score. Kaplan-Meier analyzed the overall survival (OS). Next, receiver operating characteristic (ROC) curve verified the predictive accuracy of the model. Furthermore, we performed stratification analyses to explore the relationship between the prognostic signature and clinicopathological variables. Lastly, we used another date set to verify the model. All data was processed by R (version 3.6.0) and perl (version5.18.4).Results: The K-M plot showed the overall survival rate of the high-risk group was lower than the low-risk group’s (P=2.216e−10). And Cox regression analysis suggested that the autophagy prognostic index was an independent prognostic factor. Further more, the ARGs prognostic model was confirmed in dataset of GSE65858.Conclusion: This study constructed an autophagy-related signature of oral cancer, which can foresee the prognosis of patients. It will open up new prospects for fight against oral cancer.
The MBS Microbial Rapid Detection System for rapid detection of major pathogenic bacteria in feed: Comparison with plate counting method
Authors: Jiang Linlin , Zhang Beiebei , Liu Shuitao , Zhu Lianqin , Zhu Fenghua
Published: August 2022

The current methods for detecting pathogenic bacteria in feed require high technique and take a long time. A Micro Biological Survey (MBS) rapid detection system is a simple, economical and rapid microbial detection method. The purpose of this experiment was to compare the detection ofEscherichia coli(E. coli),Salmonella,Staphylococcus aureus(S. aureus),Listeria monocytogenes(LM), coliform (COLI) and total viable count (TVC) in feed by the MBS rapid microbial detection system and plate counting method (PCM). The results showed that the MBS microbial rapid detection system could detectE. coli, Salmonella, S. aureus, LM, COLI and TVC in artificially contaminated sterilized fish meal, and the quantitative limit were 5.58 cfu/mL, 8.60 cfu/mL, 7.90 cfu/mL, 8.40 cfu/mL, 3.93 cfu/mL and 7.43 cfu/mL, respectively. The quantitative limit of the PCM were 41.30 cfu/mL, 56.70 cfu/mL, 39.00 cfu/mL, 56.00 cfu/mL, 40.00 cfu/mL and 48.00 cfu/mL, respectively. The recovery rates of the MBS microbial rapid detection system forE. coli, Salmonella, S. aureus, LM, COLI and TVC in the concentration range of 101~108cfu/mL were 87.92 ~ 98.42%, 87.19 ~ 90.76%, 89.95 ~ 96.32%, 89.15 ~ 92.93%, 90.95 ~ 97.66% and 90.21 ~ 97.03%, respectively; the recovery rates of the PCM were 5.58%~92.60%, 79.77%~93.48%, 86.33%~94.01%, 83.05%~92.69%, 87.44%~96.58% and 87.09%~94.98%, respectively. The coefficients of variation of the MBS microbial rapid detection system for detectingE. coli, Salmonella, S. aureus, LM, COLI and TVC in sterilized fish meal were 9.83%, 7.18%, 10.28%, 9.55%, 8.03% and 9.21%, respectively. The coefficients of variation of PCM were 14.92%, 13.84%, 15.63%, 12.18%, 13.21% and 14.91%, respectively. When detecting the pathogenic bacteria content in artificially contaminated feed, the MBS rapid microbial detection system was positively correlated with the PCM. When the MBS microbial rapid detection system and PCM were used to detect the collected real feed samples, there was no significant difference in the detection results of the two methods in most of the feed samples. In summary, the quantitative limit, recovery rate and coefficient of variation of the MBS microbial rapid detection system are better than the quantitative limit, recovery rate and coefficient of variation of the plate counting method, which is the most convenient and rapid detection method and is suitable for promotion and application in production lines.
Adipose tissue-derived stromal/stem cells transplantation + cholecalciferol in recent-onset type 1 diabetes patients: twelve months follow up.
Authors: DANTAS Joana Rodrigues , ARAÚJO Débora Batista , SILVA Karina Ribeiro , SOUTO Débora Lopes , PEREIRA Maria de Fátima Carvalho , LUIZ RonirRaggio , CLAUDIO-DA-SILVA Cesar , COURI Carlos Eduardo Barra , MAIOLINO Angelo , REBELATTO Carmen Lúcia Kuniyoshi , DAGA Débora Regina , SENEGAGLIA Alexandra Cristina , BROFMAN Paulo Roberto Slud , BAPTISTA Leandra S. , de OLIVEIRA José Egídio Paulo , ZAJDENVERG Lenita , RODACKI Melanie
Published: August 2022

OBJECTIVE: To evaluate safety and therapeutic effect along 12 months of allogenic adipose tissue-derived stromal/stem cells (ASCs) transplantation+cholecalciferol(VITD) in patients with recent-onset type 1 diabetes (T1D).METHODS: Prospective, phase II, open trial, pilot study in which patients with recent onset T1D received ASCs(1Kgx106 cells) and VITD 2000UI/day for 12 months(group 1) and were compared to controls with standard insulin therapy (group 2). Adverse events, C-peptide area under the curve(CPAUC), insulin dose, HbA1c and frequency of CD4+FoxP3+ T-cells(flow cytometry)were evaluated at baseline(T0), after 3(T3), 6(T6) and 12 months(T12).RESULTS: 11 patients completed the 12 months follow up (7:group 1;4:group 2). Group 1 had lower insulin requirement at T3(0.24+/-0.18vs0.53+/-0.23UI/kg,p=0.04), T6(0.24+/-0.15vs0.66 +/- 0.33 UI/kg,p=0.04) and T12(0.39+/-0.15vs0.74+/-0.29 UI/Kg,p=0.04).HbA1c was lower at T6(6.7+/-0.79vs8.75+/-0.95%,p=0.01), without significant differences at T12(7.3+/-1.11% in group 1vs8.90+/-1.33 in group 2,p=0.16).CPAUC was not significantly different at T0(p=0.07), higher in group 1 at T3(p=0.04) and T6(p=0.006), but similar at T12(p=0.23).Six patients (85,7%) in group 1 were in partial clinical remission(CR) at T6 vs none in group 2,p=0.01,4 remained in remission until 12 months. Patients with partial CR exhibited higher FOX P3 expression in CD4+lymphocytes at T6 and T12(p=0.004 and p=0.02, respectively).VITD levels were higher in patients that underwent partial CR at T6. One patient has a recurrence of a benign teratoma that was surgically removed, not associated to the intervention was observed in a patient from group 1.CONCLUSIONS: ASCs+VITD without immunosuppression was safe and associated lower insulin requirements, a better glycemic control and a transient better pancreatic function in recent onset T1D, but the potential benefits were not sustained. Trial registration : ClinicalTrial.gov NCT03920397
Origin identification of Phoebe zhennan and Phoebe bournei by SPME-GC-MS
Authors: Chen Zhenan , Wu Haoqi , Chen Ruoyi , Xue Xiaoming , Gao Handng
Published: August 2022

Phoebe zhennan S. Lee (Lauraceae) and Phoebe bournei (Hemsl.) Yang are favored for their fine-grain, insect resistance, special fragrance, not easily cracked and deformed, which had been widely used in furniture and construction. Due to the difference in wood quality characteristics and economic value of genus Phoebe species from different origins, it is important to establish a fast, efficient, and reliable method to identify the origin of Phoebe zhennan and Phoebe bournei. Solid phase micro-extraction (SPME) was used as sample preparation approach and gas chromatography-mass spectrometry (GC-MS) technique was employed to analyze the volatile compounds qualitatively. As a result, a total of 40 volatiles were identified by SPME-GC-MS among Phoebe zhennan samples from four origins, while 35 substances identified among Phoebe bournei samples. Significant difference was detected in the volatiles of Phoebe zhennan from different origins except for Guizhou and Yunnan. However, the wood samples of Phoebe bournei from all three origins could be discriminated by GC-MS spectroscopy. This finding indicated that genus Phoebe species from different origins had different chemical composition, which verified that the SPME-GC-MS technique can be used to identify different species of wood with characteristic compounds.
Consequences of an Infinite Fourier Cosine Transform-Based Ramanujan Integral
Authors: Dar S. A. , Kamarujjama M. , Shah W. M. , Daud M.
Published: August 2022

In this paper, we express a generalization of the Ramanujan integral I(α) with the analytical solutions, using the Laplace transform technique and some algebraic relation or a Pochhammer symbol. Moreover, we evaluate some consequences of a generalized definite integral φ * (υ, β , a). The well known special cases appeared whose solutions are possible by Cauchys residue theorem, and many known applications of the integral I(a, β , υ) are discussed by Leib-nitz’s rule for differentiation under the sign of integration. Further, one closed-form evaluation of the infinite series of the 1 F 0 (·) function is deduced. In addition, we establish some integral expressions in terms of the Euler numbers, which are not available in the Gradshteyn and Ryzhik book table. 2010 AMS Classification: 33C05, 33C20, 33B152010 AMS Classification: 33C05, 33C20, 33B15
Fibroblast activation protein imaging in atrial fibrillation: a proof-of-concept study
Authors: Li Lina , Gao Jie , Chen Bi-Xi , Liu Xingpeng , Shi Liang , Wang Yanjiang , Wang Li , Su Pixiong , Yang Min-Fu , Xie Boqia
Published: August 2022

Purpose: Radiolabeled fibroblast activation protein inhibitor (FAPI) imaging has proven feasible in the assessment of ventricular fibrosis in several cardiovascular diseases. However, its value in assessing atrial fibrosis in atrial fibrillation (AF) remains unknown. Methods: Twenty-eight patients with AF and 25 sex-matched controls underwent baseline FAPI PET/CT imaging. Left atrial appendage (LAA) specimens were obtained from seven patients and two lung transplant donors. Atrial pacing beagle dog AF models were constructed by pacemaker implantation. After 8 weeks of pacing, the animals underwent FAPI PET/CT imaging and specimens from the left atrial and right atrial (LA, RA) were collected. FAPI activities in the LA, RA, and LAA were measured by target-to-background ratio (TBR), and the mRNA and protein expression of fibroblast activation protein (FAP) and collagen I were measured by quantitative polymerase chain reaction (PCR) and western blot. Results: FAPI imaging identified increased atrial uptake in 17 patients with persistent AF (PsAF) (85.00%) and 5 with paroxysmal AF (PAF) (62.50%), located in the LA (64.28%), RA (57.14%), and LAA (42.86%). In all atrial structures, patients with PsAF had a higher prevalence of enhanced FAPI uptake than those with PAF (63.33% vs. 33.33%, P = 0.02). LA dilation and B-type natriuretic peptide level were determined as independent factors for increased LAA and RA FAPI uptake, respectively (OR: 1.11 and 1.02, both P < 0.05). All five beagles with AF had increased FAPI uptake in the LA and RA. For both human and animal specimens, FAPI activity was strongly associated with the mRNA and protein levels of FAP, and was closely related to the mRNA expression of collagen I. Conclusion: This preliminary study suggests that FAPI imaging is feasible for assessing activated fibroblasts in the atriums of AF.
Prediction of the incidence of colorectal cancer by routine laboratory tests and anthropometric measurements about metabolism
Authors: Cheng Binglu , Han Xu , Wan Hongjuan , Sun Zhijun , Wang Weisheng
Published: August 2022

Objective To investigate the relationship between metabolic disorders and the morbidity of colorectal cancer (CRC) compared with colorectal adenoma (CRA) and non-neoplastic inpatients, especially to investigate which anthropometric measures were better predictors of CRC. Methods 80 patients with CRC, 80 patients with CRA and 80 non-neoplastic patients were selected from April 2017 to April 2019. The data of routine laboratory tests and anthropometric measurements about metabolism were analyzed retrospectively. Multinomial Logistic regression analysis was used to estimated odds ratio (OR) and 95% confidence interval (CI) for the risk of morbidity of CRC associated with potential risk factors. Results Compared with CRA, OR of CRC was enhanced by weight loss self-reported, anemia and hypoproteinemia. Compared with non-neoplastic controls, diabetes, higher waist-neck ratio (WNR) and The Metabolic syndrome (MetS) identifified by the Chinese Diabetes Society was associated with signifificant increasing risk of all CRC patients. while in male subgroup, OR of CRC was enhanced by diabetes, higher hip circumference, waist-neck ratio (WNR)>2.42 (OR = 2.795, 95%CI: 1.169-6.680), waist-height ratio (WHtR)>0.550 (OR = 3.009, 95%CI: 1.260-7.185) and MetS. In female subgroup, only waist-hip ratio (WHR)>0.890 (multivariable-adjusted OR = 3.354, 95%CI: 1.011-11.129) was significantly associated with increased risk of CRC compared with colorectal adenoma and non-neoplastic controls. The risk factors for CRA were similar to CRC compared with non-neoplastic controls. Conclusion Visceral adipose patients have a high incidence of CRC and CRA. For male, MetS, high level WNR and WHtR caused an increasing risk for CRC. For female, Only WHR is a high-risk factor. The patients with these high risk factors need to strengthen colorectal cancer screening, which is of great significance for the prevention and early detection of colorectal cancer.
Applying the Deep Neural Network to Estimate Future Trend and Uncertainty of Rainfall under Climate Change
Authors: Lin Shiu-Shin , Yeh Wei-Li , Zhu Kai-Yang , Ho Yu-Dong , Wu Wei-Cheng
Published: August 2022

This study proposes a deep neural network (DNN) as a downscaling framework to compare original variables and nonlinear data features extracted by kernel principal component analysis (KPCA). It uses them as learning data for DNN downscaling models to assess future regional rainfall trends and uncertainties in islands with complex terrain. This study takes Taichung and Hualien in Taiwan as examples. It collects data in different emission scenarios (RCP 4.5, RCP 8.5) simulated by two Global Climate Models: ACCESS and CSMK3, in the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC), and monthly rainfall data of case regions from January 1950 to December 2005 in the Central Weather Bureau in Taiwan. DNN model parameters are optimized based on historical scenarios to estimate the trends and uncertainties of future monthly rainfall in the case regions. A multivariate linear regression is used as a baseline model to compare their effectiveness. The simulated results show that by both ACCESS and CSMK3, the dimensionless root mean squared error (RMSE) of KPCA was higher than that of the original variables in Taichung and Hualien. According to the analysis of three-class classification (according to the arrangement in descending power of historical rainfall, the predicted rainfall is divided into three ranges, low, normal, and high, marked by 30% and 70% of monthly rainfall), the wet season rainfall at the two stations is concentrated in the normal range. The probability of rainfall increase will improve in the dry season and will reduce in the wet season in the mid-term to long-term. The future wet season rainfall in Hualien has the highest variability. It ranges from 201 mm to 300 mm, with representative concentration pathways RCP 4.5 much higher than RCP 8.5. The median percentage increase and decrease in RCP 8.5 are higher than in RCP 4.5. This indicates that RCP 8.5 has a greater impact on future monthly rainfall.
Prognostic value of comprehensive typing based on m6A and gene cluster
Authors: Wu Haoming , Feng Jikun , Wu Jundong , Zhong Wenjing , Zouxu Xiazi , Huang Weiling , Huang Xinjian , Yi Jiarong , Wang Xi
Published: August 2022

Background
Triple-negative breast cancer (TNBC) is resistant to targeted therapy with HER2 monoclonal antibodies and endocrine therapy because it lacks the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). TNBC is a subtype of breast cancer with the worst prognosis and the highest mortality rate compared to other subtypes. N6-methyladenosine (m6A) modification is significant in cancer and metastasis because it can alter gene expression and function at numerous levels, such as RNA splicing, stability, translocation, and translation. There has been limited investigation into the connection between TNBC and m6A.
Materials and Methods
Breast cancer-related data were retrieved from the Cancer Genome Atlas (TCGA) database, and 116 triple-negative breast cancer cases were identified from the data. The GSE31519 dataset, which included 68 cases of TNBC, was obtained from the Gene Expression Omnibus (GEO) database. Survival analysis was used to determine the prognosis of distinct m6A types based on their m6A group, gene group, and m6A score. To investigate the potential mechanism, GO and KEGG analyses were performed on the differentially expressed genes.
Results
The expression of m6A-related genes and their impact on prognosis in TNBC patients were studied. According to the findings, m6A was crucial in determining the prognosis of TNBC patients, and the major m6A-linked genes in this process were YTHDF2, RBM15B, IGFBP3, and WTAP. By cluster analysis, the gene cluster and the m6A cluster were beneficial in predicting the prognosis of TNBC patients. The m6A score based on m6A and gene clusters was more effective in predicting the prognosis of TNBC patients. Furthermore, the tumor microenvironment may play an important role in the process of m6A, influencing TNBC prognosis.
Conclusion
N6-adenylic acid methylation (m6A) was important in altering the prognosis of TNBC patients, and the key m6A-associated genes in this process were YTHDF2, RBM15B, IGFBP3, and WTAP. Furthermore, the comprehensive typing based on m6A and gene clusters was useful in predicting TNBC patients' prognosis, showing potential as a meaningful evaluating tools for TNBC.
Hydro-chemical studies and assessment of trace elements and bacterial contamination of shallow groundwater of Oyo area, southwestern Nigeria
Authors: Akanbi Olanrewaju Akinfemiwa , Akinola Oluwagbemileke Samuel , Nwajei Jeremiah , Adegbite Joshua
Published: August 2022

Groundwater from various shallow hand-dug wells is the only reliable source of water supply in the ancient town of Oyo in southwestern Nigeria. The present work studied the water quality in twenty-five wells across this area by measuring the ancillary parameters, and analyzing the major and trace elements and taking the inventory of the wells as well as assessing the total coliform and Escherichia coliform in sampled water. The field measurement showed that the groundwater is slightly acidic with an average pH of 6.6 and fresh from the total dissolved solids values between 70 and 630 mg/L. From the average concentrations of major ions, the dominance order of the cations constituents in groundwater is in the order- Mg2+ > Ca2+ > K+ > Na+ and HCO- > CI- > NO3- > SO42- for anions. The ranges of trace elements concentrations were; Iron II- 0.01 – 0.06; Chromium VI: 0 - 0.34; manganese 0 – 1.2 in mg/L, while Copper was between 0.38 – 32.7; Cadmium 0.03 – 1.87; lead 0.02 – 3.57; Zinc 0.01 – 7.79; and Arsenic 0.05 – 7.35 in µg/L. The total coliform count ranges from 6 – 1860 cfu/100 mL while Escherichia coliform (E. coli) units ranges from nil in three wells representing 12% of the wells, 1 – 50 units (28%), 51 – 100 (20%), 101 – 500 (20%) and > 500 units (20%). The groundwater is grossly polluted with nitrate and coliform bacteria while four wells were found to be contaminated with manganese and chromium.
Can benefit equity alleviate health inequity? --Evidence from the reform of Health Insurance Programs in China
Authors: Li Rui , Wu Jing , Lu Jiaqian
Published: August 2022

The higher the socioeconomic status, the better the health status. Can the equity reform of health insurance alleviate the health inequality between different groups? Taking the provincial-level coordination reform of Chinese Urban Employee Basic Medical Insurance (UEBMI) as an example, the paper used the China Family Panel Studies (CFPS) data and relevant policy documents to examine the effect of equalized health insurance programs on health inequity by Recentered Influence Function (RIF). The empirical results showed that although the gap in medical reimbursement costs between participants of different socioeconomic status was narrowing after the provincial-level coordination reform, the health inequality among the insured population widened. The equalized health insurance reform can’t alleviate the health inequality brought about by socioeconomic status. This paper suggests that in the progress of promoting the provincial-level coordination reform of UEBMI, the protection of vulnerable groups should be strengthened to promote social equity.JEL codes: I10, I14, I30
FeOOH nanolayers decorated on oxygen-vacancy-containing BiVO 4 for efficient photoelectrochemical degradation of Rhodamine B
Authors: Ma Mingyang , Ruan Mengnan , Nie Weixing
Published: August 2022

The short carrier diffusion length, the trade-off between sufficient light absorption and efficient charge separation of BiVO4 photoelectrode often leads to poor photoelectrochemical (PEC) degradation performance. In this paper, we firstly designed a method of combining oxygen vacancies (Ov) with cocatalyst FeOOH for synergistic improve BiVO4 PEC degradation performance. After introducing Ov resulted from hydrothermal method, the photocurrent density value reaches 0.54 mA/cm2 at 1.23 V vs. RHE by the enhanced of carrier density and the broaden of light absorption, 1.38 times higher than that of pure BiVO4 (0.39 mA/cm2 at 1.23 V vs. RHE). As expected, the as-synthesized BiVO4-Ov/FeOOH exhibits enhanced stability and a remarkable photocurrent density of 1.18 mA cm2 at 1.23 V vs. RHE under AM 1.5G illumination. In addition, a significant PEC degradation performance of 85% were reached, which is about 1.7 times than BiVO4-Ov. The results demonstrate that the Ovs can be effectively preserved by the coating of a thin FeOOH layer, leading to a photoelectrode of enhanced photocurrent and stability.