bluuebunny/crossref_metadata_2025 huggingface dataset:
Construction of machine learning-based models for cancer outcomes in low and lower-middle income countries: A scoping review
Authors: Adeoye John , Akinshipo Abdulwarith , Koohi-Moghadam Mohamad , Thomson Peter , Su Yu-Xiong
Published: December 2022

Background

The impact and utility of machine learning (ML)-based prediction tools for cancer outcomes including assistive diagnosis, risk stratification, and adjunctive decision-making have been largely described and realized in the high income and upper-middle-income countries. However, statistical projections have estimated higher cancer incidence and mortality risks in low and lower-middle-income countries (LLMICs). Therefore, this review aimed to evaluate the utilization, model construction methods, and degree of implementation of ML-based models for cancer outcomes in LLMICs.

Methods

PubMed/Medline, Scopus, and Web of Science databases were searched and articles describing the use of ML-based models for cancer among local populations in LLMICs between 2002 and 2022 were included. A total of 140 articles from 22,516 citations that met the eligibility criteria were included in this study.

Results

ML-based models from LLMICs were often based on traditional ML algorithms than deep or deep hybrid learning. We found that the construction of ML-based models was skewed to particular LLMICs such as India, Iran, Pakistan, and Egypt with a paucity of applications in sub-Saharan Africa. Moreover, models for breast, head and neck, and brain cancer outcomes were frequently explored. Many models were deemed suboptimal according to the Prediction model Risk of Bias Assessment tool (PROBAST) due to sample size constraints and technical flaws in ML modeling even though their performance accuracy ranged from 0.65 to 1.00. While the development and internal validation were described for all models included (n=137), only 4.4% (6/137) have been validated in independent cohorts and 0.7% (1/137) have been assessed for clinical impact and efficacy.

Conclusion

Overall, the application of ML for modeling cancer outcomes in LLMICs is increasing. However, model development is largely unsatisfactory. We recommend model retraining using larger sample sizes, intensified external validation practices, and increased impact assessment studies using randomized controlled trial designs

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=308345, identifier CRD42022308345.
Simultaneous CSM‐TACE with CalliSpheres® and partial splenic embolization using 8spheres® for hepatocellular carcinoma with hypersplenism: Early prospective multicenter clinical outcome
Authors: Zhou Jun , Feng Zhuo , Liu Song , Li Xiang , Liu Ying , Gao Fei , Shen Jing , Zhang Yue Wei , Zhao Guang Sheng , Zhang Ming
Published: December 2022

Background

Primary hepatocellular carcinoma is often complicated with hepatitis and liver cirrhosis. Some patients develop different degrees of splenomegaly, hypersplenism and hypohepatia due to the aggravation of liver cirrhosis, which to some extent interfere with the treatment of tumors and even affect the prognosis of patients. In this study, we prospectively evaluate the efficacy and safety of simultaneous CalliSpheres® microspheres transcatheter arterial chemoembolization (CSM-TACE) and partial splenic embolization (PSE) using 8spheres® for hepatocellular carcinoma (HCC) with hypersplenism.

Methods

Ninety consecutive HCC patients with hypersplenism who underwent CSM-TACE were selected: 32 patients in CSM-TACE+PSE group, and 58 patients in CSM-TACE group. The peripheral blood cell counts (leukocyte, platelet (PLT), liver function and red blood cell (RBC)), CSM-TACE and/or PSE related complications, and the tumor control rate at 1 month after CSM-TACE were compared. The survival time and prognostic factors were also observed.

Results

Before CSM-TACE, there were no significant differences in sex, age, Child-Pugh grade, tumor size, and alpha-fetoprotein (AFP) between the two groups. After CSM-TACE, the PLT and white blood cell (WBC) counts in CSM-TACE+PSE group were significantly higher than those in the CSM-TACE group (P<0.05). There were no significant differences in RBC before and after treatment (P > 0.05). In the CSM-TACE group, there were no significant differences in WBC, PLT, and RBC before and after treatment (P > 0.05). There was no significant difference in liver function at 1 month after treatment between the two groups. The cholinesterase (CHE) level in the CSM-TACE+PSE group after CSM-TACE+PSE was obviously higher than that before CSM-TACE+PSE and higher than that in the CSM-TACE group (P<0.05). However, the level of CHE returned to the preoperative level 1 month after CSM-TACE in the CSM-TACE group. The objective response rate (ORR) and median overall survival (OS) in the CSM-TACE+PSE group were higher than those in the CSM-TACE group (P<0.05). The adverse reactions of the two groups were fever, abdominal pain, stomach discomfort, nausea, and vomiting, and no serious complications occurred. The degree of abdominal pain and fever in the experimental group was lower than that in the control group (P > 0.05).

Conclusions

Simultaneous CSM-TACE and PSE using domestic embolization particles for HCC with hypersplenism have good safety and efficacy and has a low incidence of PSE-related adverse events, it is conducive to improving liver function reserve, and can further improve the median OS.
Organizational characteristics of European pediatric onco-critical care: An international cross-sectional survey
Authors: Nielsen Jeppe S. A. , Agbeko Rachel , Bate Jessica , Jordan Iolanda , Dohna-Schwake Christian , Potratz Jenny , Moscatelli Andrea , Bottari Gabriella , Pappachan John , Witt Volker , Crazzolara Roman , Amigoni Angela , Mizia-Malarz Agniezka , Sánchez Martín Mariá , Willems Jef , van den Heuvel-Eibrink Marry M. , Schlapbach Luregn J. , Wösten-van Asperen Roelie M. ,   the POKER (PICU Oncology Kids in Europe Research group) research consortium of ESPNIC (European Society of Paediatric & Neonatal Intensive Care)
Published: December 2022

Background

Intensified treatment protocols have improved survival of pediatric oncology patients. However, these treatment protocols are associated with increased treatment-related morbidity requiring admission to pediatric intensive care unit (PICU). We aimed to describe the organizational characteristics and processes of care for this patient group across PICUs in Europe.

Methods

A web-based survey was sent to PICU directors or representative physicians between February and June 2021.

Results

Responses were obtained from 77 PICUs of 12 European countries. Organizational characteristics were similar across the different countries of Europe. The median number of PICU beds was 12 (IQR 8–16). The majority of the PICUs was staffed by pediatric intensivists and had a 24/7 intensivist coverage. Most PICUs had a nurse-to-patient ratio of 1:1 or 1:2. The median numbers of yearly planned and unplanned PICU admissions of pediatric cancer patients were 20 (IQR 10–45) and 10 (IQR 10–30, respectively. Oncology specific practices within PICU were less common in participating centres. This included implementation of oncology protocols in PICU (30%), daily rounds of PICU physicians on the wards (13%), joint mortality and morbidity meetings or complex patients’ discussions (30% and 40%, respectively) and participation of parents during clinical rounds (40%).

Conclusion

Our survey provides an overview on the delivery of critical care for oncology patients in PICU across European countries. Multidisciplinary care for these vulnerable and challenging patients remains complex and challenging. Future studies need to determine the effects of differences in PICU organization and processes of care on patients’ outcome.
MM/PB(GB)SA benchmarks on soluble proteins and membrane proteins
Authors: Wang Shiyu , Sun Xiaolin , Cui Wenqiang , Yuan Shuguang
Published: December 2022

Predicting protein-ligand binding free energy rapidly and accurately remains a challenging question in modern drug discovery. Molecular mechanics/Poisson-Boltzmann (Generalized Born) surface area (MM/PB(GB)SA) has emerged as an essential tool for accelerating cost-efficient binding free energy calculation. This study presents benchmarks with three membrane-bound protein systems and six soluble protein systems. Different parameters were sampled for different benchmarks to explore the highest accuracy. These include ligand charges, protein force fields, extra points, GB models, nonpolar optimization methods, internal dielectric constants and membrane dielectric constants. Comparisons of accuracy were made between MM/PB(GB)SA, docking and free energy perturbation (FEP). The results reveal a competitive performance between MM/PB(GB)SA and FEP. In summary, MM/PB(GB)SA is a powerful approach to predict ligand binding free energy rapidly and accurately. Parameters of MM/PB(GB)SA calculations, such as the GB models and membrane dielectric constants, need to be optimized for different systems. This method can be served as a powerful tool for drug design.
Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex
Authors: Feng Yongmei , Grotegut Stefan , Jovanovic Predrag , Gandin Valentina , Olson Steven H. , Murad Rabi , Beall Anne , Colayco Sharon , De-Jesus Paul , Chanda Sumit , English Brian P. , Singer Robert H. , Jackson Michael , Topisirovic Ivan , Ronai Ze’ev A.
Published: December 2022

The translation initiation complex 4F (eIF4F) is a rate-limiting factor in protein synthesis. Alterations in eIF4F activity are linked to several diseases, including cancer and infectious diseases. To this end, coronaviruses require eIF4F complex activity to produce proteins essential for their life cycle. Efforts to target coronaviruses by abrogating translation have been largely limited to repurposing existing eIF4F complex inhibitors. Here, we report the results of a high throughput screen to identify small molecules that disrupt eIF4F complex formation and inhibit coronavirus RNA and protein levels. Of 338,000 small molecules screened for inhibition of the eIF4F-driven, CAP-dependent translation, we identified SBI-1232 and two structurally related analogs, SBI-5844 and SBI-0498, that inhibit human coronavirus OC43 (HCoV-OC43; OC43) with minimal cell toxicity. Notably, gene expression changes after OC43 infection of Vero E6 or A549 cells were effectively reverted upon treatment with SBI-5844 or SBI-0498. Moreover, SBI-5844 or SBI-0498 treatment effectively impeded the eIF4F complex assembly, with concomitant inhibition of newly synthesized OC43 nucleocapsid protein and OC43 RNA and protein levels. Overall, we identify SBI-5844 and SBI-0498 as small molecules targeting the eIF4F complex that may limit coronavirus transcripts and proteins, thereby representing a basis for developing novel therapeutic modalities against coronaviruses.
Non-coding RNA-related antitumor mechanisms of marine-derived agents
Authors: Zhou Zhixia , Cao Qianqian , Diao Yujing , Wang Yin , Long Linhai , Wang Shoushi , Li Peifeng
Published: December 2022

In the last two decades, natural active substances have attracted great attention in developing new antitumor drugs, especially in the marine environment. A series of marine-derived compounds or derivatives with potential antitumor effects have been discovered and developed, but their mechanisms of action are not well understood. Emerging studies have found that several tumor-related signaling pathways and molecules are involved in the antitumor mechanisms of marine-derived agents, including noncoding RNAs (ncRNAs). In this review, we provide an update on the regulation of marine-derived agents associated with ncRNAs on tumor cell proliferation, apoptosis, cell cycle, invasion, migration, drug sensitivity and resistance. Herein, we also describe recent advances in marine food-derived ncRNAs as antitumor agents that modulate cross-species gene expression. A better understanding of the antitumor mechanisms of marine-derived agents mediated, regulated, or sourced by ncRNAs will provide new biomarkers or targets for potential antitumor drugs from preclinical discovery and development to clinical application.
Targeting hypoxia-inducible factors for breast cancer therapy: A narrative review
Authors: Luo Shuang , Jiang Yu , Anfu Zheng  , Zhao Yueshui , Wu Xu , Li Mingxing , Du Fukuan , Chen Yu , Deng Shuai , Chen Meijuan , Li Wanping , Li Xiaobing , Gu Li , Sun Yuhong , Xiao Zhangang , Shen Jing
Published: December 2022

Hypoxia-inducible factors (HIFs), central regulators for cells to adapt to low cellular oxygen levels, are often overexpressed and activated in breast cancer. HIFs modulate the primary transcriptional response of downstream pathways and target genes in response to hypoxia, including glycolysis, angiogenesis and metastasis. They can promote the development of breast cancer and are associated with poor prognosis of breast cancer patients by regulating cancer processes closely related to tumor invasion, metastasis and drug resistance. Thus, specific targeting of HIFs may improve the efficiency of cancer therapy. In this review, we summarize the advances in HIF-related molecular mechanisms and clinical and preclinical studies of drugs targeting HIFs in breast cancer. Given the rapid progression in this field and nanotechnology, drug delivery systems (DDSs) for HIF targeting are increasingly being developed. Therefore, we highlight the HIF related DDS, including liposomes, polymers, metal-based or carbon-based nanoparticles.
Zhuanggu Guanjie herbal formula mitigates osteoarthritis via the NF-κB transduction mechanism
Authors: Guowei Gong , Yuzhong Zheng , Xuan Zhou , Zhi Dai , Juanhui Duan , Jing Wang , Peikui Yang , Xiangzhi Liu , Zhen Wen
Published: December 2022

The Zhuanggu Guanjie herbal formula has been a famous Chinese prescription for treating bone diseases since time immemorial. The anti-osteoarthritis (OA) properties of this botanical prescription are well documented in the Chinese Pharmacopoeia. However, the detailed mechanisms behind the phenomenon have not been elucidated. Hence, we aimed to investigate the anti-OA efficacy of the Zhuanggu Guanjie herbal formula and its underlying mechanism. The anti-OA properties of Zhuanggu Guanjie capsule (ZGC) were determined by the cytokine contents and inflammatory-related proteins, which were measured by RT-PCR, flow cytometry, Western blot, and laser confocal assay in ATDC5 cells. The levels of interleukin-6, tumor necrosis factor-α, inducible nitric oxide synthase, cyclooxygenase-2, and prostaglandin synthesis E2 have been markedly reduced after being treated with ZGC for 48 h in a dose-dependent manner. Furthermore, ZGC prevented the translocation of NF-κB from the cytosol to the nucleus. On the other hand, we used the mono-iodoacetate (MIA)-induced OA model to confirm thein vivoefficacies of this herbal formula. Oral administration of ZGC attenuated MIA-induced OA damage through changes in histopathological and knee joint volumes. The serum matrix metalloproteinase-13 contents in the ZGC treatment group declined as compared to those in the MIA model group. Through ourin vitroandin vivostudies, we confirmed the anti-OA efficacy of ZGC and uncovered its detailed mechanism, and this treatment shed light on OA pathophysiology.
178-W picosecond green laser with active beam-pointing stabilization
Authors: Zhang Zhendong , Bai Zhenao , Song Xiaoquan , Zhang Yakai , Liang Bo , Liu Tingxia , Wang Weiguo
Published: December 2022

Picosecond lasers with high average power and high beam quality have been widely used for precision processing and space exploration. In this study, we report a high-power picosecond green laser using a multistage Yb-doped rod-shaped photonic crystal fiber as an amplifier combined with a beam combination. The single amplification module achieves a 1,030 nm laser output of 146.8 W, and the maximum second harmonic generation (SHG) power is 92 W with a frequency conversion efficiency of 63.5%. The combined beam of the two SHGs resulted in a final output of 178 W with a repetition frequency of 24.07 MHz, pulse width of 50.1 ps, and beam quality factor of M2 = 1.16. Furthermore, an adaptive filter control method of a two-axis fast-steering mirror was applied to suppress the beam jitter to up to 45 Hz.
Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis
Authors: Wu Baowei , Zhang Xiaoyu , Hu Kunzhi , Zheng Haoyuan , Zhang Siyu , Liu Xiangli , Ma Meng , Zhao Huixian
Published: December 2022

In wheat production, appropriate flowering time and ideal plant architecture are the prerequisites for high grain yield. Alternative splicing (AS) is a vital process that regulates gene expression at the post-transcriptional level, and AS events in wheat have been found to be closely related to grain-related traits and abiotic stress tolerance. However, AS events and their biological roles in regulating flowering time and plant architecture in wheat remain unclear. In this study, we report that TaNAK1 undergoes AS, producing three splicing variants. Molecular characterization of TaNAK1 and its splicing variants demonstrated that all three protein isoforms have a conserved NB-ARC domain and a protein kinase domain, but the positions of these two domains and the length of the protein kinase domains are different among them, implying that they may have different three-dimensional structures and therefore have different functions. Further investigations showed that the two splicing variants of TaNAK1, TaNAK1.1 and TaNAK1.2, exhibited different expression patterns during wheat growth and development, while the other one, TaNAK1.3, was not detected. Subcellular localization demonstrated that TaNAK1.1 was mainly localized in the cytoplasm, while TaNAK1.2 was localized in the nucleus and cytoplasm. Both TaNAK1.1 and TaNAK1.2 exhibit protein kinase activity in vitro. Ectopic expression of TaNAK1.1 and TaNAK1.2 in Arabidopsis demonstrated that these two splicing variants play opposite roles in regulating flowering time and plant architecture, resulting in different seed yields. TaNAK1.2 positive regulates the transition from vegetative to reproductive growth, plant height, branching number, seed size, and seed yield of Arabidopsis, while TaNAK1.1 negatively regulates these traits. Our findings provide new gene resource for regulating flowering time and plant architecture in crop breeding for high grain yield.
Inter-row information recognition of maize in the middle and late stages via LiDAR supplementary vision
Authors: Li Zhiqiang , Xie Dongbo , Liu Lichao , Wang Hai , Chen Liqing
Published: December 2022

In the middle and late stages of maize, light is limited and non-maize obstacles exist. When a plant protection robot uses the traditional visual navigation method to obtain navigation information, some information will be missing. Therefore, this paper proposed a method using LiDAR (laser imaging, detection and ranging) point cloud data to supplement machine vision data for recognizing inter-row information in the middle and late stages of maize. Firstly, we improved the YOLOv5 (You Only Look Once, version 5) algorithm based on the characteristics of the actual maize inter-row environment in the middle and late stages by introducing MobileNetv2 and ECANet. Compared with that of YOLOv5, the frame rate of the improved YOLOv5 (Im-YOLOv5) increased by 17.91% and the weight size decreased by 55.56% when the average accuracy was reduced by only 0.35%, improving the detection performance and shortening the time of model reasoning. Secondly, we identified obstacles (such as stones and clods) between the rows using the LiDAR point cloud data to obtain auxiliary navigation information. Thirdly, the auxiliary navigation information was used to supplement the visual information, so that not only the recognition accuracy of the inter-row navigation information in the middle and late stages of maize was improved but also the basis of the stable and efficient operation of the inter-row plant protection robot was provided for these stages. The experimental results from a data acquisition robot equipped with a camera and a LiDAR sensor are presented to show the efficacy and remarkable performance of the proposed method.
Automatic instance segmentation of orchard canopy in unmanned aerial vehicle imagery using deep learning
Authors: Zhang Weirong , Chen Xuegeng , Qi Jiangtao , Yang Sisi
Published: December 2022

The widespread use of unmanned aerial vehicles (UAV) is significant for the effective management of orchards in the context of precision agriculture. To reduce the traditional mode of continuous spraying, variable target spraying machines require detailed information about tree canopy. Although deep learning methods have been widely used in the fields of identifying individual trees, there are still phenomena of branches extending and shadows preventing segmenting edges of tree canopy precisely. Hence, a methodology (MPAPR R-CNN) for the high-precision segment method of apple trees in high-density cultivation orchards by low-altitude visible light images captured is proposed. Mask R-CNN with a path augmentation feature pyramid network (PAFPN) and PointRend algorithm was used as the base segmentation algorithm to output the precise boundaries of the apple tree canopy, which addresses the over- and under-sampling issues encountered in the pixel labeling tasks. The proposed method was tested on another miniature map of the orchard. The average precision (AP) was selected to evaluate the metric of the proposed model. The results showed that with the help of training with the PAFPN and PointRend backbone head that AP_seg and AP_box score improved by 8.96% and 8.37%, respectively. It can be concluded that our algorithm could better capture features of the canopy edges, it could improve the accuracy of the edges of canopy segmentation results.
Phylogenomic analyses based on the plastid genome and concatenated nrDNA sequence data reveal cytonuclear discordance in genus Atractylodes (Asteraceae: Carduoideae)
Authors: Liu Jinxin , Shi Mengmeng , Zhang Zhaolei , Xie Hongbo , Kong Weijun , Wang Qiuling , Zhao Xinlei , Zhao Chunying , Lin Yulin , Zhang Xiaoxia , Shi Linchun
Published: December 2022

Atractylodes species are widely distributed across East Asia and are cultivated as medicinal herbs in China, Japan, and Korea. Their unclear morphological characteristics and low levels of genetic divergence obscure the taxonomic relationships among these species. In this study, 24 plant samples were collected representing five species of Atractylodes located in China; of these, 23 belonged to members of the A. lancea complex. High-throughput sequencing was used to obtain the concatenated nrDNA sequences (18S-ITS1-5.8S-ITS2-28S) and plastid genomes. The concatenated nrDNA sequence lengths for all the Atractylodes species were 5,849 bp, and the GC content was 55%. The lengths of the whole plastid genome sequences ranged from 152,138 bp (A. chinensis) to 153,268 bp (A. lancea), while their insertion/deletion sites were mainly distributed in the intergenic regions. Furthermore, 33, 34, 36, 31, and 32 tandem repeat sequences, as well as 30, 30, 29, 30, and 30 SSR loci, were detected in A. chinensis, A. koreana, A. lancea, A. japonica, and A. macrocephala, respectively. In addition to these findings, a considerable number of heteroplasmic variations were detected in the plastid genomes, implying a complicated phylogenetic history for Atractylodes. The results of the phylogenetic analysis involving concatenated nrDNA sequences showed that A. lancea and A. japonica formed two separate clades, with A. chinensis and A. koreana constituting their sister clade, while A. lancea, A. koreana, A. chinensis, and A. japonica were found based on plastid datasets to represent a mixed clade on the phylogenetic tree. Phylogenetic network analysis suggested that A. lancea may have hybridized with the common ancestor of A. chinensis and A. japonica, while ABBA–BABA tests of SNPs in the plastid genomes showed that A. chinensis was more closely related to A. japonica than to A. lancea. This study reveals the extensive discordance and complexity of the relationships across the members of the A. lancea complex (A. lancea, A. chinensis, A. koreana, and A. japonica) according to cytonuclear genomic data; this may be caused by interspecific hybridization or gene introgression.
Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism
Authors: Prasad C. T. Manjunath , Kodde Jan , Angenent Gerco C. , de Vos Ric C. H. , Diez-Simon Carmen , Mumm Roland , Hay Fiona R. , Siricharoen Sasiwimon , Yadava Devendra K. , Groot Steven P. C.
Published: December 2022

Seed aging during storage results in loss of vigor and germination ability due to the accumulation of damage by oxidation reactions. Experimental aging tests, for instance to study genetic variation, aim to mimic natural aging in a shorter timeframe. As the oxidation rate is increased by elevating the temperature, moisture, and oxygen levels, this study aimed to (1) investigate the effect of experimental rice seed aging by an elevated partial pressure of oxygen (EPPO), (2) elucidate the mechanism of dry-EPPO aging and (3) compare aging under dry-EPPO conditions to aging under traditional moist-controlled deterioration (CD) conditions and to long-term ambient storage. Dry seeds from 20 diverse rice accessions were experimentally aged under EPPO (200 times higher oxygen levels), at 50% relative humidity (RH), along with storage under high-pressure nitrogen gas and ambient conditions as controls. While no decline in germination was observed with ambient storage, there was significant aging of the rice seeds under EPPO storage, with considerable variation in the aging rate among the accessions, with an average decline toward 50% survival obtained after around 21 days in EPPO storage and total loss of germination after 56 days. Storage under high-pressure nitrogen gas resulted in a small but significant decline, by an average of 5% germination after 56 days. In a second experiment, seven rice seed lots were stored under EPPO as compared to a moist-CD test and two different long-term ambient storage conditions, i.e., conditioned warehouse seed storage (CWSS) and traditional rice seed storage (TRSS). Untargeted metabolomics (with identification of lipid and volatile compounds profiles) showed a relatively high increase in levels of oxidized lipids and related volatiles under all four storage conditions. These compounds had a high negative correlation with seed viability, indicating oxidation as a main deteriorating process during seed aging. Correlation analysis indicated that EPPO storage at 50% RH is more related to aging under TRSS at 60% and CD-aging at 75% ERH rather than CWSS at 40% ERH. In conclusion, aging rice seeds under EPPO conditions is a suitable experimental aging method for analyzing variation among seed lots or genotypes for longevity under storage.
Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
Authors: Baró Aina , Saldarelli Pasquale , Saponari Maria , Montesinos Emilio , Montesinos Laura
Published: December 2022

Transient expression of genes encoding peptides BP134 and BP178 by means of a Potato virus X (PVX) based-vector system, and treatment with synthetic peptides by endotherapy, were evaluated in the control of Xylella fastidiosa infections, in the model plant Nicotiana benthamiana. Transient production of BP178 significantly decreased disease severity compared to PVX and non-treated control (NTC) plants, without adverse effects. Plants treated with synthetic BP134 and BP178 showed consistently lower levels of disease than NTC plants. However, the coinfection with PVX-BP134 and X. fastidiosa caused detrimental effects resulting in plant death. The levels of X. fastidiosa in three zones sampled, upwards and downwards of the inoculation/treatment point, significantly decreased compared to the NTC plants, after the treatment with BP178, but not when BP178 was produced transiently. The effect of treatment and transient production of BP178 in the induction of defense-related genes was also studied. Synthetic BP178 applied by endotherapy induced the expression of ERF1, PR1a, PAL, PALII and WRKY25, while the transient expression of BP178 overexpressed the Cath, Cyc, PR4a, 9-LOX and Endochitinase B genes. Both treatments upregulated the expression of PR1, PR3, PR4 and CycT9299 genes compared to the NTC or PVX plants. It was concluded that the effect of BP178, either by endotherapy or by transient expression, on the control of the X. fastidiosa infections in N. benthamiana, was due in part to the induction of the plant defense system in addition to its bactericidal activity reported in previous studies. However, the protection observed when BP178 was transiently produced seems mainly mediated by the induction of plant defense, because the levels of X. fastidiosa were not significantly affected.
GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
Authors: Yan Mengyuan , Yu Xiaotian , Zhou Gen , Sun Dongli , Hu Yu , Huang Chenjue , Zheng Qintao , Sun Nan , Wu Jiayan , Fu Zhaobin , Li Libei , Feng Zhen , Yu Shuxun
Published: December 2022

Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved CDPKs in Gossypium hirsutum L. were identified and characterized, which was divided into four subgroups. Among them, the transcript level of GhCDPK60 was significantly upregulated under drought and several hormone treatments. And we found that the expression levels of several stress-inducible genes down-regulated in GhCDPK60-silence cotton and up-regulated in GhCDPK60-overexpressing Arabidopsis. In addition, physiological analyses demonstrated that GhCDPK60 improved drought stress tolerance by improving the osmotic adjustment ability and reducing the accumulation of reactive oxygen species (ROS) in plants. These findings broaden our understanding of the biological roles of GhCDPK60 and mechanisms underlying drought stress tolerance in cotton.
Citric acid assisted phytoextraction of nickle from soil helps to tolerate oxidative stress and expression profile of NRAMP genes in sunflower at different growth stages
Authors: Ijaz Munazza , Ansari Mahmood-ur-Rahman , Alafari Hayat Ali , Iqbal Muhammad , Alshaya Dalal S. , Fiaz Sajid , Ahmad Hafiz Muhammad , Zubair Muhammad , Ramzani Pia Muhammad Adnan , Iqbal Javed , Abushady Asmaa M. , Attia Kotb
Published: December 2022

Introduction

Soil polluted with Nickel (Ni) adversely affects sunflower growth resulting in reduced yield. Counterbalancing Ni toxicity requires complex molecular, biochemical, and physiological mechanisms at the cellular, tissue, and whole plant levels, which might improve crop productivity. One of the primary adaptations to tolerate Ni toxicity is the enhanced production of antioxidant enzymes and the elevated expression of Ni responsive genes.

Methods

In this study, biochemical parameters, production of ROS, antioxidants regulation, and expression ofNRAMPmetal transporter genes were studied under Ni stress in sunflower. There were four soil Ni treatments (0, 50, 100, and 200 mg kg-1soil), while citric acid (CA, 5 mM kg-1soil) was applied on the 28thand 58thdays of plant growth. The samples for all analyses were obtained on the 30thand 60thday of plant growth, respectively.

Results and discussion

The results indicated that the concentrations of Ni in roots and shoots were increased with increasing concentrations of Ni at both time intervals. Proline contents, ascorbic acid, protein, and total phenolics were reduced under Ni-stress, but with the application of CA, improvement was witnessed in their contents. The levels of malondialdehyde and hydrogen peroxide were enhanced with the increasing concentration of Ni, and after applying CA, they were reduced. The contents of antioxidants, i.e., catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase, were increased at 50 ppm Ni concentration and decreased at higher concentrations of Ni. The application of CA significantly improved antioxidants at all concentrations of Ni. The enhanced expression ofNRAMP1(4, 51 and 81 folds) andNRAMP3(1.05, 4 and 6 folds) was found at 50, 100 and 200ppm Ni-stress, respectively in 30 days old plants and the same pattern of expression was recorded in 60 days old plants. CA further enhanced the expression at both developmental stages.

Conclusion

In conclusion, CA enhances Ni phytoextraction efficiency as well as protect plant against oxidative stress caused by Ni in sunflower.
Arbuscular mycorrhizal fungi enhance disease resistance of Salvia miltiorrhiza to Fusarium wilt
Authors: Pu Chunjuan , Ge Yang , Yang Guang , Zheng Han , Guan Wei , Chao Zhi , Shen Ye , Liu Sha , Chen Meilan , Huang Luqi
Published: December 2022

Salvia miltiorrhiza Bunge (Danshen in Chinese) is vulnerable to Fusarium wilt, which severely affects the quality of the crude drug. Mycorrhizal colonization enhances resistance to fungal pathogens in many plant species. In this study, pre-inoculation of S. miltiorrhiza with the arbuscular mycorrhizal fungi (AMF) Glomus versiforme significantly alleviated Fusarium wilt caused by Fusarium oxysporum. Mycorrhizal colonization protected S. miltiorrhiza from pathogen infection, thereby preventing a loss of biomass and photosynthesis. There were greater defense responses induced by pathogen infection in AMF pre-inoculated plants than those in non-treated plants. AMF pre-inoculation resulted in systemic responses upon pathogen inoculation, including significant increases in the protein content and activities of phenylalanine ammonia-lyase (PAL), chitinase, and β-1,3-glucanase in S. miltiorrhiza roots. In addition, mycorrhizal pre-inoculation caused upregulation of defense-related genes, and jasmonic acid (JA) and salicylic acid (SA) signaling pathway genes after pathogen infection. The above findings indicate that mycorrhizal colonization enhances S. miltiorrhiza resistance against F. oxysporum infection by enhancing photosynthesis, root structure, and inducing the expression of defense enzymes and defense-related genes on the other hand.
The scar that takes time to heal: A systematic review of COVID-19-related stigma targets, antecedents, and outcomes
Authors: Zhou Xiang , Chen Chen , Yao Yuewei , Xia Jingtian , Cao Limei , Qin Xin
Published: December 2022

COVID-19, as a crucial public health crisis, has affected our lives in nearly every aspect. Besides its major health threats, COVID-19 brings severe secondary impacts, one of which is the rise of social stigma. Although numerous studies have examined the antecedents and outcomes of COVID-19-related stigma, we still lack a systematic understanding of who is being stigmatized during the COVID-19 pandemic, what exacerbates COVID-19-related stigma, and what impacts COVID-19-related stigma has on victims. Therefore, this review aims to provide a systematic overview of COVID-19-related stigma. With 93 papers conducted with 126,371 individuals in more than 150 countries and territories spanning five continents, we identify three targets that have received the most research: Chinese/Asian people, (suspected) patients and survivors, and healthcare workers. Furthermore, we find that for each stigma target, characteristics of the stigmatized, stigmatizer, and context contribute to COVID-19-related stigma and that this stigma negatively influences victims' health and non-health outcomes. We call for future research to provide a more integrative, balanced, and rigorous picture of COVID-19-related stigma via conducting research on neglected topics (e.g., contextual factors that contribute to stigma toward HCWs) and stigma interventions and using a longitudinal design. In practice, we urge governments and institutions (e.g., ministries of public health, hospitals) to pay close attention to stigma issues and to promote safe and inclusive societies.
Climate values as predictor of climate change perception in the Kingdom of Saudi Arabia
Authors: Arnout Boshra A.
Published: December 2022

Background

Understanding public perceptions of climate change and how individuals perceive it is critical to developing effective communication strategies, policies, and socially robust technologies to relieve the risks of climate change. Despite the growing literature on climate change, until now, researchers in Arab countries have not been interested in studying citizens’ perceptions of climate change or identifying the factors that predict it. This study aimed to identify and understand the nature and dynamics of public perceptions of climate change among Arab citizens and detect the level of climate change perception (CCP) and climate values (CV). Also, to detect the predictability of CCP from CV. As well as to reveal the differences between CCP and CV.

Methods

A random sample consisted of 465 participants (236 male and 229 female), residents of the Kingdom of Saudi Arabia; their ages ranged from 30 years and over. The Climate Change Perception Questionnaire (CCPQ) and Climate Values Questionnaire (CVQ) were applied online.

Results

The results found average levels of CCP and CV among the study sample. The results also revealed significant statistical differences in the CCP and CV due to gender in favor of females. As well as, there were significant statistical differences in the CCP due to the career field in favor of agriculture, engineering, and construction workers. Also, there were statistical differences in the emotional subscale of climate perception and CV due to age groups in favor of individuals whose ages ranged from 30 to 45 years. The results also found that the CV were a statistically significant predictor (1.2% of total variance) of climate perception.

Conclusion

The current study showed an average level of CCP and CV among individuals in the Kingdom of Saudi Arabian. The findings also concluded that individuals’ perceptions of climate change are an individual response determined by the individual’s gender, age, and career field and are affected by his values about climate. These findings shed light on the need for climate communications to increase the level of CCP and CV, especially among males and individuals in the age group over 45 years and individuals working in various career fields, whether education, engineering and construction, and commerce and business, etc.; to improve the engagement in mitigation and adaptation measures to climate change.
Research on the state of blended learning among college students – A mixed-method approach
Authors: Deng Chao , Peng Jiao , Li ShuFei
Published: December 2022

In the wake of the COVID-19 pandemic in 2019, China’s education leaders began to focus on and promote blended learning. The process is still in its infancy in Chinese colleges and universities, and its development remains a problem to be solved. By combining technology acceptance and student participation, this article proposes an analysis model for assessing the factors influencing blended learning. A questionnaire was designed and distributed, and 796 valid responses were collected. The mean and variance were used to examine the status of students’ technology acceptance and satisfaction with blended learning. The t-test method was employed to analyze the gender differences between students in regard to the topic. The results show that: (1) students majoring in computer science view the factors as having a high level of influence in blended learning. (2) There are major variances regarding the perception of service quality between male and female computer science major students. There is no significant difference between them in terms of perceived usefulness, perceived ease of use, or computer self-efficacy. (3) There are considerable disparities in the skill involvement and participation of computer science major college students. The results show that the technology acceptance and participation of students determine the effect of blended learning. Based on these findings, this article provides theoretical and practical suggestions for the implementation of blended learning to improve its effect.
Can second language metaphorical competence be taught through instructional intervention? A meta-analysis
Authors: Zhou Xiaoyong , Younas Muhammad , Omar Abdulfattah , Guan Lu
Published: December 2022

Background

For a long time, the traditional view regarded metaphor as merely a rhetorical device that served to enrich linguistic expression. With the continuous development of cognitive linguistics, foreign language educators began to realize the vital role of metaphor in foreign language education.

Objectives

This study looked at how well pedagogical interventions improve metaphorical competence by looking at how well teachers teach metaphors.

Methods

After a rigorous literature search and selection process from the Chinese and English databases, 13 Chinese and 7 international studies with 51 effect sizes were included in this meta-analysis. With the help of the meta-analysis 3.0 software, the literature and heterogeneity tests were performed to ensure that the meta-analysis results were as accurate and valid as possible.

Results

The effect size tests revealed that the metaphorical instructional intervention was significantly effective in general and produced a large effect size (d = 0.888) on improving learners' metaphorical abilities. Meta-regression analyses were also conducted to examine how other factors might change the effects of the interventions.

Findings

Research has shown that instructional interventions that combine prolonged input of metaphorical concepts with reinforcement of metaphorical practice can help second language learners develop metaphorical competence. Teaching puts more pressure on teachers and the learning environment, and the results of this study could help teachers teach metaphors in the future.
What drives me to use TikTok: A latent profile analysis of users’ motives
Authors: Gu Li , Gao Xun , Li Yong
Published: December 2022

With TikTok rising in social media, the heterogeneity of users creates diversity in motives for using it. The issue of which profiles of TikTok use motives can be identified warrants greater understanding. Conducting latent profile analyses for a sample of 384 TikTok users, we identified four profiles, namely overall low motives, overall medium motives, overall high motives, and escapist addiction and novelty motives profiles. The former three profiles reflect different levels of motivations across four motives (socially rewarding self-presentation, trendiness, escapist addiction, and novelty). It is worth noting that TikTok users in the escapist addiction and novelty motives profile are mainly motivated by escapist addiction and novelty, but not socially rewarding self-presentation or trendiness motives. Through multivariate analysis of variance (MANOVA) and multinomial logistic regression, we further explore the differences in TikTok use between the profiles and the extent to which users’ background characteristics and TikTok use predict their profile membership. The four profiles differ significantly in terms of the scores of almost all TikTok use motives. The frequency of TikTok use and video posting are the most notable predictors of profile membership. These results make theoretical contributions to the extant literature on social media use profiles by exploring the number and types of latent profiles of TikTok use motives, which also inform opportunities for enhancing user experience and imply tailored content recommendations to both achieve maximized gratifications and maintain mental wellbeing based on user profiles.
The after-school sedentary behavior status among children and adolescents with intellectual disabilities
Authors: Yuan Yaqing , Ding Jianing , Wang Chao , Zhang Shaohua , Wang Yinping , Liu Yang , Liu Jingmin
Published: December 2022

Background

There is evidence that the after-school period plays an essential role in accumulating sedentary behavior (SB) among children and adolescents, as well as implementing potential interventions. However, relatively little is known regarding SB status of children and adolescents with intellectual disabilities (ID) during the after-school period. The purpose of this study was to investigate the total level and specific pattern of the after-school SB among children and adolescents with ID.

Methods

The after-school SB status among 325 children and adolescents with ID was evaluated by the parent-reported Children’s Leisure Activities Study Survey-Chinese edition questionnaire.

Results

Parents of children and adolescents with ID reported approximately 204 min/day of after-school SB. Specifically, the longest time of the after-school period was spent performing the screen-based SB (84 mins/d). This was followed by recreational SB and educational SB (50 and 30 mins/d, respectively). The children aged 6–12 years old engaged more time in recreational SB than adolescents aged 16–18 years old (p < 0.05) during the after-school period. Further, the data indicated that 37.5% of children and adolescents with ID achieved the guideline limitation of 2-h-maximum screen-based SB during the after-school hour.

Conclusion

Children and adolescents with ID spent a large portion of the after-school period in SB, particularly engaged in more time on after-school screen-based SB. Future efforts should focus on developing and implementing period-specific interventions designed to reduce after-school SB in the segment of this population.
Seroepidemiology of pertussis in the east of China: Estimates of incidence of infection in adolescents and adults pre- and post-COVID-19
Authors: Chen Qiang , Wang Wen , Shi Xiuyun , Xu Yan , Zhu Yanhong , Wu Yun , Wang Zhiguo , Sun Hui , Sun Xiang
Published: December 2022

Introduction

The dramatic decrease in the number of reported cases of pertussis during COVID-19 pandemic has been underestimated. The objective was to compare the estimated incidence rate of pertussis in populations pre- and post-COVID-19 pandemic by analyzing the anti-pertussis toxin (anti-PT) IgG and anti-filamentous hemagglutininant (anti-FHA) IgG antibodies in healthy Chinese population from 2018 to 2021.

Methods

All serum samples (N = 1,000) were collected from healthy population (aged ≥ 15 years) who attended an annual monitoring project of antibody levels in Jiangsu province in 2018–2021 were measured by ELISA.

Results

The positive rates of anti-PT IgG and anti-FHA IgG antibodies were 11.4% (114/1,000) and 20.2% (202/1,000) (≥40 IU/ml), the GMC were 17.25 (95% CI: 15.49–19.03) IU/mL and 24.94 (95% CI: 22.73–27.16) IU/mL in the study population, respectively. The percentage of participants with anti-PT IgG antibodies higher than 40 IU/mL was 5.20% (11/212) in 2018, 5.5% (19/348) in 2019, 21.2% (46/217) in 2020 and 17.0% (38/223) in 2021, respectively. The non-detectable rate (<5 IU/mL) of anti-PT IgG antibodies was 16.9, 17.7, 28.1, and 37.3% in 2018, 2019, 2020, and 2021, respectively. We assumed that the infection occurred within 58.6 days, and based on the overall proportion (2.9%) of individuals with anti-PT IgG antibody ≥100 IU/ml, the incidence rate (/100) was estimated by the formula to be 18.08 (95% CI: 12.40–26.11). In addition, the estimated incidence of Post-COVID-19 was higher than that of Pre-COVID-19 (36.33/100 vs. 12.84/100), and the difference was statistically significant (p < 0.05).

Conclusions

Our results suggest a high rate of under-reporting of pertussis in Jiangsu Province both pre- and post-COVID-19 pandemic, and there are a large number of adults of childbearing age who are susceptible to pertussis. It seems imperative that vaccination of adolescents and adults should be considered for inclusion in vaccination programs.