bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the perceptual attribution of a virtual robotic limb synchronizing with hand and foot simultaneously
Authors: Sakurada Kuniharu , Kondo Ryota , Nakamura Fumihiko , Kitazaki Michiteru , Sugimoto Maki
Published: November 2023

Introduction:Incorporating an additional limb that synchronizes with multiple body parts enables the user to achieve high task accuracy and smooth movement. In this case, the visual appearance of the wearable robotic limb contributes to the sense of embodiment. Additionally, the user’s motor function changes as a result of this embodiment. However, it remains unclear how users perceive the attribution of the wearable robotic limb within the context of multiple body parts (perceptual attribution), and the impact of visual similarity in this context remains unknown.

Methods:This study investigated the perceptual attribution of a virtual robotic limb by examining proprioceptive drift and the bias of visual similarity under the conditions of single body part (synchronizing with hand or foot motion only) and multiple body parts (synchronizing with average motion of hand and foot). Participants in the conducted experiment engaged in a point-to-point task using a virtual robotic limb that synchronizes with their hand and foot motions simultaneously. Furthermore, the visual appearance of the end-effector was altered to explore the influence of visual similarity.

Results:The experiment revealed that only the participants’ proprioception of their foot aligned with the virtual robotic limb, while the frequency of error correction during the point-to-point task did not change across conditions. Conversely, subjective illusions of embodiment occurred for both the hand and foot. In this case, the visual appearance of the robotic limbs contributed to the correlations between hand and foot proprioceptive drift and subjective embodiment illusion, respectively.

Discussion:These results suggest that proprioception is specifically attributed to the foot through motion synchronization, whereas subjective perceptions are attributed to both the hand and foot.
Thermal fluctuations, QNMs, and emission energy of charged ADS black hole with nonlinear electrodynamics
Authors: Javed Faisal , Basit Abdul , Caliskan Aylin , Güdekli Ertan
Published: November 2023

This study examines the thermodynamics of charged anti-de Sitter (AdS) black holes (BHs) with nonlinear electrodynamics (NED) using quasinormal modes (QNMs) and thermal fluctuations. For this purpose, we calculate the Hawking temperature and discuss the stable configuration of the considered black hole using heat capacity. First, we study the interesting aspects of the emission of energy. Then, we explore the effects of thermal corrections on thermodynamic quantities and their corrected energies. We study the phase transitions of the system in the background of thermal fluctuations. It is concluded that the presence of a coupling constant enhances the thermodynamically stable configuration of uncharged and charged AdS BH geometries. We highlight that our results are in good agreement with the thermodynamics of the previous black hole solutions and assumptions presented in the literature.
Temporal and spatial evolution of agricultural carbon emissions and their impact on functional zoning: evidence from Hubei Province
Authors: Chen Qiao , Mao Yan , Cheng Jianquan
Published: November 2023

Introduction

To investigate the spatiotemporal evolution of agricultural carbon emissions and carbon absorption, analyse the spatiotemporal variations in the carbon balance, delineate carbon-offsetting regions, and formulate low-carbon development strategies tailored to various major functional zones, this study aims to promote coordinated regional ecological and environmental governance.

Methods

This study takes a perspective based on major functional zones, focuses on 17 cities in Hubei Province, studies the spatiotemporal variations in agricultural carbon budgets and carbon offsets in each city from the perspective of functional zoning and proposes a spatial optimization scheme for reducing carbon emissions.

Results and discussion

The results show that both agricultural carbon emissions and carbon absorption in Hubei Province gradually increased, although the agricultural carbon budgets varied significantly among cities. Arable lands were the main agricultural carbon sinks in Hubei Province. Overall, carbon emissions exhibited declining core–periphery zonation, with Xiangyang, Jingzhou, and Huanggang serving as the centre (high emissions) and the cities of Shennongjia, Enshi, and Yichang serving as the periphery (low emissions). Carbon absorption displayed a U-shaped distribution, with high values in the east, south, and west and low values in the centre and north. The cities of Yichang, Jingmen, and Huanggang were the peak carbon sink areas. In recent years, the coordination between the agricultural carbon emissions and carbon budgets in Hubei Province has gradually improved, and agricultural carbon absorption and emissions have become increasingly balanced. Seven carbon-positive, five carbon-neutral, and five carbon-negative areas were identified in the province. Based on these findings, differentiated carbon emission reduction strategies were proposed to promote coordinated and low-carbon agriculture.
Cytomegalovirus Cell-Mediated Immunity: Ready for Routine Use?
Authors: Bestard Oriol , Kaminski Hannah , Couzi Lionel , Fernández-Ruiz Mario , Manuel Oriol
Published: November 2023

Utilizing assays that assess specific T-cell-mediated immunity against cytomegalovirus (CMV) holds the potential to enhance personalized strategies aimed at preventing and treating CMV in organ transplantation. This includes improved risk stratification during transplantation compared to relying solely on CMV serostatus, as well as determining the optimal duration of antiviral prophylaxis, deciding on antiviral therapy when asymptomatic replication occurs, and estimating the risk of recurrence. In this review, we initially provide an overlook of the current concepts into the immune control of CMV after transplantation. We then summarize the existent literature on the clinical experience of the use of immune monitoring in organ transplantation, with a particular interest on the outcomes of interventional trials. Current evidence indicates that cell-mediated immune assays are helpful in identifying patients at low risk for replication for whom preventive measures against CMV can be safely withheld. As more data accumulates from these and other clinical scenarios, it is foreseeable that these assays will likely become part of the routine clinical practice in organ transplantation.
Thoughts on the Importance of Similitude and Multi-Axial Loads When Assessing the Durability and Damage Tolerance of Adhesively-Bonded Doublers and Repairs
Authors: Jones Rhys , Chandwani Ramesh , Timbrell Chris , Kinloch Anthony J. , Peng Daren
Published: November 2023

Adhesively bonded doublers and adhesively bonded repairs are extensively used to extend the operational life of metallic aircraft structures. Consequently, this paper focuses on the tools needed to address sustainment issues associated with both adhesively bonded doublers and adhesively bonded repairs to (metallic) aircraft structures, in a fashion that is consistent with the building-block approach mandated in the United States Air Force (USAF) airworthiness certification standard MIL-STD-1530D and also in the United States (US) Joint Services Structural Guidelines JSSG-2006. In this context, it is shown that the effect of biaxial loads on cohesive crack growth in a bonded doubler under both constant amplitude fatigue loads and operational flight loads can be significant. It is also suggested that as a result, for uniaxial tests to replicate the cohesive crack growth seen in adhesively bonded doublers and adhesively bonded repairs under operational flight loads, the magnitude of the applied load spectrum may need to be continuously modified so as to ensure that the crack tip similitude parameter in the laboratory tests reflects that seen in the full-scale aircraft.
Phenolic Profile and Bioactivity Changes of Lotus Seedpod and Litchi Pericarp Procyanidins: Effect of Probiotic Bacteria Biotransformation
Authors: Wen Junren , Sui Yong , Li Shuyi , Shi Jianbin , Cai Sha , Xiong Tian , Cai Fang , Zhou Lei , Zhao Shengnan , Mei Xin
Published: November 2023

Theoretically, lactic acid bacteria (LABs) could degrade polyphenols into small molecular compounds. In this study, the biotransformation of lotus seedpod and litchi pericarp procyanidins by Lactobacillus plantarum 90 (Lp90), Streptococcus thermophilus 81 (ST81), Lactobacillus rhamnosus HN001 (HN001), and Pediococcus pentosus 06 (PP06) were analysed. The growth curve results indicated that procyanidins did not significantly inhibit the proliferation of LABs. Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UPLC–HRMS) revealed that procyanidin B2 and procyanidin B3 in lotus seedpod decreased by 62.85% and 25.45%, respectively, with ST81 metabolised, while kaempferol and syringetin 3-O-glucoside content increased. Although bioconversion did not increase the inhibitory function of procyanidins against glycosylation end-products in vitro, the 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) free radical scavenging capacity and ferric reducing antioxidant power of litchi pericarp procyanidins increased by 157.34% and 6.8%, respectively, after ST81 biotransformation. These findings may inspire further studies of biological metabolism of other polyphenols and their effects on biological activity.
Correction: Cárdenas-Castillo, L.F.; Camacho-Guardian, A. Strongly Interacting Bose Polarons in Two-Dimensional Atomic Gases and Quantum Fluids of Polaritons. Atoms 2023, 11, 3
Authors: Cárdenas-Castillo Luis Fernando , Camacho-Guardian Arturo
Published: November 2023

The authors wish to make the following corrections to their paper [...]
Phenolic Compounds Stability of Grumixama (Eugenia brasiliensis) Juice during Processing and Storage
Authors: Modesto Junior Elivaldo Nunes , Chaves Rosane Patricia Ferreira , Martins Mayara Galvão , Pereira Gustavo Araujo , Chisté Renan Campos , Pena Rosinelson da Silva
Published: November 2023

Grumixama is an anthocyanin-rich berry commonly found in South America, yet not widely consumed by Brazilians due to the lack of studies covering its technological properties. Therefore, the stability of anthocyanins and the activity of oxidoreductase enzymes in grumixama juice during thermal treatment (60 °C to 100 °C) and storage (at 25 °C and 7 °C) were evaluated. As a result, the pasteurization of grumixama juice at 80 °C for 60 s completely inactivated peroxidase (POD) and polyphenol oxidase (PPO), eliminated microorganisms, and induced low degradation of the total anthocyanins (40%) and minimal degradation of the total phenolic compounds. Four anthocyanins were monitored in the pasteurized juice, namely delphinidin 3-glucoside, cyanidin 3-glucoside, and two unidentified anthocyanins. Despite the temperature range, the qualitative chromatographic profile of the anthocyanins did not change, while the contents were affected. The binominal temperature/time of 80 °C/60 s effectively inactivated PPO and POD enzymes, promoted moderate alterations in the contents of total anthocyanins and total phenolic compounds, and ensured the microbiological quality of grumixama juice. Grumixama juice can be used as a health food due to its high content of antioxidant phenolic compounds, especially anthocyanins.
Identification of the Mixing Ratios of Beer-Mixed Beverages by UV/VIS Spectroscopy
Authors: Stinus Sophia , Erban Pauline , Diebold Josef , Schmidt Magnus S.
Published: November 2023

UV/VIS spectroscopy was used in conjunction with a calibration method to determine the mixing ratio of samples of beer-mixed drinks with mineral water (sour Radler beers) from the food service industry. For this purpose, calibration lines were constructed based on absorptions at the two wavelengths 380 nm and 430 nm. To create these calibration lines, blending ratios were prepared from beers from three southwestern German brands and mineral water. The samples of the sour Radler beers were taken from restaurants and bars, which are all located in Baden-Württemberg. It has been shown that the calibration lines can be used to determine mixing ratios with an average deviation of approximately 7% and 9%. Thereby, the calibration line based on the absorption at 430 nm represents a higher accuracy. Using this calibration line, the sour Radler beer samples have percentage ranges of beer of approximately 50% to 90%. This method could serve as a rapid quantitative quality control of the mixing ratios of sour Radler or other beer-mixed drinks. It could be used in breweries for routine control.
Controlling Trophoblast Cell Fusion in the Human Placenta—Transcriptional Regulation of Suppressyn, an Endogenous Inhibitor of Syncytin-1
Authors: Sugimoto Jun , Schust Danny J. , Sugimoto Makiko , Jinno Yoshihiro , Kudo Yoshiki
Published: November 2023

Cell fusion in the placenta is tightly regulated. Suppressyn is a human placental endogenous retroviral protein that inhibits the profusogenic activities of another well-described endogenous retroviral protein, syncytin-1. In this study, we aimed to elucidate the mechanisms underlying suppressyn’s placenta-specific expression. We identified the promoter region and a novel enhancer region for the gene encoding suppressyn, ERVH48-1, and examined their regulation via DNA methylation and their responses to changes in the oxygen concentration. Like other endogenous retroviral genes, the ERVH48-1 promoter sequence is found within a characteristic retroviral 5′ LTR sequence. The novel enhancer sequence we describe here is downstream of this LTR sequence (designated EIEs: ERV internal enhancer sequence) and governs placental expression. The placenta-specific expression of ERVH48-1 is tightly controlled by DNA methylation and further regulated by oxygen concentration-dependent, hypoxia-induced transcription factors (HIF1α and HIF2α). Our findings highlight the involvement of (1) tissue specificity through DNA methylation, (2) expression specificity through placenta-specific enhancer regions, and (3) the regulation of suppressyn expression in differing oxygen conditions by HIF1α and HIF2α. We suggest that these regulatory mechanisms are central to normal and abnormal placental development, including the development of disorders of pregnancy involving altered oxygenation, such as preeclampsia, pregnancy-induced hypertension, and fetal growth restriction.
Signs of Alveolar Collapse in Idiopathic Pulmonary Fibrosis, Hypersensitivity Pneumonitis and Systemic Sclerosis Revealed by Inspiration and Expiration Computed Tomography
Authors: Wittwer Marco Fabian , Kim Soung-Yung , Leichtle Alexander , Berezowska Sabina , Guler Sabina A. , Geiser Thomas , Heverhagen Johannes , Maurer Britta , Poellinger Alexander
Published: November 2023

Idiopathic pulmonary fibrosis (IPF), hypersensitivity pneumonitis (HP) and systemic sclerosis (SSc) are among the most common entities that cause pulmonary fibrosis. Alveolar collapse with subsequent collapse induration of lung tissue is thought to contribute to the fibrotic transformation. The purpose of this study was to examine lung tissue in computed tomography (CT) of non-diseased appearance during expiration for signs of increased density suggesting collapsibility in fibrosing lung diseases. We further analyzed the diaphragmatic movements during the respiratory cycle to determine relationships between density differences and the apex–diaphragm diameter. Significant differences in attenuation changes between inspiration and expiration of unaffected lung parenchyma were detected between IPF and controls and between HP and controls for all lung lobes (p < 0.001). Only minor differences were found between SSc and controls. There was no clinically relevant difference between patients with IPF and those with HP. The measured absolute apex–diaphragm diameter in inspiration and expiration demonstrated a statistically significant difference between patients with IPF versus normal controls. However, the diaphragmatic excursions were not different between these groups. Compared to controls, CT lung density increases significantly more during expiration in the fibrotic lungs of IPF and HP patients. The observed increase in density might indicate the collapse of alveoli during expiration and may represent a common pathophysiologic feature of fibrosing lung diseases. The density changes and lung extensions do not have the same ratios across different diseases and controls.
Correlation of Diffusion Tensor Tractography with Restless Legs Syndrome Severity
Authors: Park Kang Min , Kim Keun Tae , Lee Dong Ah , Cho Yong Won
Published: November 2023

This prospective study investigated white matter tracts associated with restless legs syndrome (RLS) severity in 69 patients with primary RLS using correlational tractography based on diffusion tensor imaging. Fractional anisotropy (FA) and quantitative anisotropy (QA) were analyzed separately to understand white matter abnormalities in RLS patients. Connectometry analysis revealed positive correlations between RLS severity and FA values in various white matter tracts, including the left and right cerebellum, corpus callosum forceps minor and major, corpus callosum body, right cingulum, and frontoparietal tract. In addition, connectometry analysis revealed that the FA of the middle cerebellar peduncle, left inferior longitudinal fasciculus, left corticospinal tract, corpus callosum forceps minor, right cerebellum, left frontal aslant tract, left dentatorubrothalamic tract, right inferior longitudinal fasciculus, left corticostriatal tract superior, and left cingulum parahippocampoparietal tract was negatively correlated with RLS severity in patients with RLS. However, there were no significant correlations between QA values and RLS severity. It is implied that RLS symptoms may be potentially reversible with appropriate treatment. This study highlights the importance of considering white matter alterations in understanding the pathophysiology of RLS and in developing effective treatment strategies.
Mechanisms of Sustained Increases in γ Power Post-Ketamine in a Computational Model of the Hippocampal CA3: Implications for Ketamine’s Antidepressant Mechanism of Action
Authors: Petzi Maximilian , Singh Selena , Trappenberg Thomas , Nunes Abraham
Published: November 2023

Subanaesthetic doses of ketamine increase γ oscillation power in neural activity measured using electroencephalography (EEG), and this effect lasts several hours after ketamine administration. The mechanisms underlying this effect are unknown. Using a computational model of the hippocampal cornu ammonis 3 (CA3) network, which is known to reproduce ketamine’s acute effects on γ power, we simulated the plasticity of glutamatergic synapses in pyramidal cells to test which of the following hypotheses would best explain this sustained γ power: the direct inhibition hypothesis, which proposes that increased γ power post-ketamine administration may be caused by the potentiation of recurrent collateral synapses, and the disinhibition hypothesis, which proposes that potentiation affects synapses from both recurrent and external inputs. Our results suggest that the strengthening of external connections to pyramidal cells is able to account for the sustained γ power increase observed post-ketamine by increasing the overall activity of and synchrony between pyramidal cells. The strengthening of recurrent pyramidal weights, however, would cause an additional phase shifted voltage increase that ultimately reduces γ power due to partial cancellation. Our results therefore favor the disinhibition hypothesis for explaining sustained γ oscillations after ketamine administration.
Navigating the Gene Co-Expression Network and Drug Repurposing Opportunities for Brain Disorders Associated with Neurocognitive Impairment
Authors: Manuel Mathew Timothy Artuz , Tayo Lemmuel L.
Published: November 2023

Neurocognitive impairment refers to a spectrum of disorders characterized by a decline in cognitive functions such as memory, attention, and problem-solving, which are often linked to structural or functional abnormalities in the brain. While its exact etiology remains elusive, genetic factors play a pivotal role in disease onset and progression. This study aimed to identify highly correlated gene clusters (modules) and key hub genes shared across neurocognition-impairing diseases, including Alzheimer’s disease (AD), Parkinson’s disease with dementia (PDD), HIV-associated neurocognitive disorders (HAND), and glioma. Herein, the microarray datasets AD (GSE5281), HAND (GSE35864), glioma (GSE15824), and PD (GSE7621) were used to perform Weighted Gene Co-expression Network Analysis (WGCNA) to identify highly preserved modules across the studied brain diseases. Through gene set enrichment analysis, the shared modules were found to point towards processes including neuronal transcriptional dysregulation, neuroinflammation, protein aggregation, and mitochondrial dysfunction, hallmarks of many neurocognitive disorders. These modules were used in constructing protein-protein interaction networks to identify hub genes shared across the diseases of interest. These hub genes were found to play pivotal roles in processes including protein homeostasis, cell cycle regulation, energy metabolism, and signaling, all associated with brain and CNS diseases, and were explored for their drug repurposing experiments. Drug repurposing based on gene signatures highlighted drugs including Dorzolamide and Oxybuprocaine, which were found to modulate the expression of the hub genes in play and may have therapeutic implications in neurocognitive disorders. While both drugs have traditionally been used for other medical purposes, our study underscores the potential of a combined WGCNA and drug repurposing strategy for searching for new avenues in the simultaneous treatment of different diseases that have similarities in gene co-expression networks.
Construction of a Nomogram to Predict Overall Survival in Patients with Early-Onset Hepatocellular Carcinoma: A Retrospective Cohort Study
Authors: Kuang Tianrui , Ma Wangbin , Zhang Jiacheng , Yu Jia , Deng Wenhong , Dong Keshuai , Wang Weixing
Published: November 2023

Hepatocellular carcinoma (HCC) is a widespread and impactful cancer which has pertinent implications worldwide. Although most cases of HCC are typically diagnosed in individuals aged ≥60 years, there has been a notable rise in the occurrence of HCC among younger patients. However, there is a scarcity of precise prognostic models available for predicting outcomes in these younger patients. A retrospective analysis was conducted to investigate early-onset hepatocellular carcinoma (EO-LIHC) using data from the Surveillance, Epidemiology, and End Results (SEER) database from 2004 to 2018. The analysis included 1392 patients from the SEER database and our hospital. Among them, 1287 patients from the SEER database were assigned to the training cohort (n = 899) and validation cohort 1 (n = 388), while 105 patients from our hospital were assigned to validation cohort 2. A Cox regression analysis showed that age, sex, AFP, grade, stage, tumor size, surgery, and chemotherapy were independent risk factors. The nomogram developed in this study demonstrated its discriminatory ability to predict the 1-, 3-, and 5-year overall survival (OS) rates in EO-LIHC patients based on individual characteristics. Additionally, a web-based OS prediction model specifically tailored for EO-LIHC patients was created and validated. Overall, these advancements contribute to improved decision-making and personalized care for individuals with EO-LIHC.
MicroRNA Profiling of Red Blood Cells for Lung Cancer Diagnosis
Authors: Geng Xinyan , Ma Jie , Dhilipkannah Pushpa , Jiang Feng
Published: November 2023

Background: Despite extensive endeavors to establish cell-free circulating biomarkers for lung cancer diagnosis, clinical adoption remains elusive. Noteworthy, emergent evidence suggests the pivotal roles of red blood cells (RBCs) and their derivatives in tumorigenesis, illuminating potential avenues for diagnostic advancements using blood cell-derived microRNAs (miRNAs). Methods: We executed microarray analyses on three principal blood cell types—RBCs, peripheral blood mononuclear cells (PBMCs), and neutrophils—encompassing 26 lung cancer patients and 26 healthy controls. Validation was performed using droplet digital PCR within an additional cohort comprising 42 lung cancer and 39 control cases. Results: Our investigation unearthed distinct miRNA profiles associated with lung cancer across all examined blood cell types. Intriguingly, RBC-miRNAs emerged as potential novel biomarkers for lung cancer, an observation yet to be documented. Importantly, integrating miRNAs from disparate blood cell types yielded a superior diagnostic accuracy for lung cancer over individual cell-type miRNAs. Subsequently, we formulated three diagnostic panels, adeptly discerning non-small cell lung cancer, adenocarcinoma, and squamous cell carcinoma, maintaining consistency across various disease stages. Conclusion: RBC-derived molecules introduce novel cancer biomarkers, and exploiting miRNA profiles across varied blood cell types unveils a promising frontier for lung cancer’s early detection and histological classification.
Long Non-Coding RNA LOC339059 Attenuates IL-6/STAT3-Signaling-Mediated PDL1 Expression and Macrophage M2 Polarization by Interacting with c-Myc in Gastric Cancer
Authors: Han Haibo , Ding Guangyu , Wang Shanshan , Meng Junling , Lv Yunwei , Yang Wei , Zhang Hong , Wen Xianzi , Zhao Wei
Published: November 2023

Background: Long non-coding RNA (lncRNA) was identified as a novel diagnostic biomarker in gastric cancer (GC). However, the functions of lncRNAs in immuno-microenvironments have not been comprehensively explored. In this study, we explored a critical lncRNA, LOC339059, that can predict the clinical prognosis in GC related to the modulation of PD-L1 and determined its influence upon macrophage polarization via the IL-6/STAT3 pathway. Methods: To date, accumulating evidence has demonstrated that the dysregulation of LOC339059 plays an important role in the pathological processes of GC. It acts as a tumor suppressor, regulating GC cell proliferation, migration, invasion, tumorigenesis, and metastasis. A flow cytometry assay showed that the loss of LOC339059 enhanced PDL1 expression and M2 macrophage polarization. RNA sequencing, RNA pull-down, RNA immunoprecipitation, Chip-PCR, and a luciferase reporter assay revealed the pivotal role of signaling alternation between LOC339059 and c-Myc. Results: A lower level of LOC339059 RNA was found in primary GC tissues compared to adjacent tissues, and such a lower level is associated with a poorer survival period (2.5 years) after surgery in patient cohorts. Moreover, we determined important immunological molecular biomarkers. We found that LOC339059 expression was correlated with PD-L1, CTLA4, CD206, and CD204, but not with TIM3, FOXP3, CD3, C33, CD64, or CD80, in a total of 146 GC RNA samples. The gain of LOC339059 in SGC7901 and AGS inhibited biological characteristics of malignancy, such as proliferation, migration, invasion, tumorigenesis, and metastasis. Furthermore, our data gathered following the co-culture of THP-1 and U937 with genomic GC cells indicate that LOC339059 led to a reduction in the macrophage cell ratio, in terms of CD68+/CD206+, to 1/6, whereas the selective knockdown of LOC339059 promoted the abovementioned malignant cell phenotypes, suggesting that it has a tumor-suppressing role in GC. RNA-Seq analyses showed that the gain of LOC339059 repressed the expression of the interleukin family, especially IL-6/STAT3 signaling. The rescue of IL-6 in LOC339059-overexpressing cells reverted the inhibitory effects of the gain of LOC339059 on malignant cell phenotypes. Our experiments verified that the interaction between LOC339059 and c-Myc resulted in less c-Myc binding to the IL-6 promoter, leading to the inactivation of IL-6 transcription. Conclusions: Our results establish that LOC339059 acts as a tumor suppressor in GC by competitively inhibiting c-Myc, resulting in diminished IL-6/STAT3-signaling-mediated PDL1 expression and macrophage M2 polarization.
Surgery in Bilateral Wilms Tumor—A Single-Center Experience
Authors: Souza Fernanda Kelly Marques de , Fanelli Mayara Caroline Amorim , Duarte Alexandre Alberto Barros , Alves Maria Teresa de Seixas , Lederman Henrique Manoel , Cypriano Monica dos Santos , Abib Simone de Campos Vieira
Published: November 2023

The treatment of bilateral Wilms tumors (BWT) involves curing the cancer, preserving long-term renal function, and maintaining a good quality of life. Established methods for achieving these goals include preoperative chemotherapy and nephron-sparing surgery (NSS). This study aimed to evaluate the experience of a single institution in treating patients with BWT. We analyzed cases of BWT treated at the Pediatric Oncology Institute—GRAACC—Federal University of São Paulo over a period of 35 years. Bleeding control was performed with manual compression of the renal parenchyma. Thirty-three patients were included in the study. Thirty cases were synchronous tumors. The mean age at diagnosis was 30.4 months (±22 m) and 66.7% were girls. The median follow-up period was 83 months. Neoadjuvant chemotherapy was the primary approach in most patients (87.9%), with a simultaneous upfront surgical approach performed in 84.8%. Most patients underwent bilateral NSS (70.4%). There were no early complications in this series, but 39.4% had clinical complications. The five-year survival rate was 76%. Therefore, it is clear that the surgical approach to BWT plays a crucial role in achieving good outcomes. However, it is difficult to standardize surgical techniques and technology may have the potential to enhance safety.
An Asymptomatic, Ectopic Mass as a Presentation of Adrenocortical Carcinoma Due to a Novel Germline TP53 p.Phe338Leu Tetramerisation Domain Variant
Authors: Walenciak Justyna , Urbanska Zuzanna , Pastorczak Agata , Babol-Pokora Katarzyna , Wypyszczak Kamila , Bien Ewa , Gawlowska-Marciniak Aleksandra , Kobos Jozef , Grajkowska Wieslawa , Smyczynska Joanna , Mlynarski Wojciech , Janczar Szymon
Published: November 2023

Adrenocortical carcinoma (ACC) is a rare cancer in childhood. ACC is frequently associated with germline TP53 variants, with founder effects especially due to the p.Arg337His mutation. ACC leads to the secretion of adrenocortical hormones, resulting in endocrine syndromes, which is the usual trigger for establishing the diagnosis. We present a surprising ACC pathology in a non-secreting, ectopic retroperitoneal tumour in a 4-year-old boy, successfully controlled with chemotherapy and mitotane after microscopically incomplete tumour resection with spillage. Genomic analysis (gene panel sequencing and copy-number microarray) demonstrated a novel p.Phe338Leu tetramerisation domain (TD) TP53 variant in the proband and his cancer-free mother and a monoallelic deletion encompassing the TP53 locus in cancer tissue, consistent with cancer-predisposition syndrome. While the recurrent p.Arg337His variant translates into high ACC risk, residue 338 and, in general, TD domain variants drive heterogeneous clinical scenarios, despite generally being considered less disruptive than TP53 DNA-binding domain mutations.
Study on Single-Layer and Single-Channel Microstructure of 304 Stainless Steel Using Joule Heat Additive Manufacturing
Authors: Li Suli , Gao Zhuang , Xiong Jie , Fan Longfei , Chen Jichao , Ma Kaiyue , Yang Laixia , Lu Bingheng
Published: November 2023

In this study, a solution to the issue of a large heat-affected zone in Wire Arc Additive Manufacturing is presented by employing the Joule Heat Additive Manufacturing method to create a single layer and single channel with a reduced heat-affected zone. The microstructure of the single layer and single channel is thoroughly investigated using various detection methods, including optical microscopy (OM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and electron backscatter diffraction (EBSD). The results reveal that the heat-affected zone formed by the Joule Heat Additive Manufacturing method is smaller than that produced by the Wire Arc Additive Manufacturing method. Additionally, the grains in the single layer and single channel progress from planar to columnar, then equiaxed, and finally back to columnar from the fusion line to the top of the wire. The element content and distribution are relatively uniform. The microstructure of the single layer consists of austenite and a small amount of ferrite, with austenite accounting for 99.71% of the content. The grain size in the middle of the wire is mainly around 10 μm, with the smallest angle grain boundaries within 10°. The distribution of local grain orientation differences in the three regions is found to be largely consistent. The analysis of the microstructure of the single layer and single channel serves as a valuable reference for understanding the behavior of single-channel multi-layers in future studies.
The Cell Wall-Related Gene Families of Wheat (Triticum aestivum)
Authors: Penning Bryan W.
Published: November 2023

Wheat crops provide 20% of calories worldwide. Cell walls function in plant growth, are part of biotic and abiotic stress resistance, and provide plant mechanical strength and adaptability. These functions factor into the productivity of wheat. The genes that produce and maintain the plant cell wall are up to 10% of the genome in many varied families. Previously, curated cell wall gene families have been published for maize and rice, two other important crop grasses. Here, 81 cell wall-related wheat gene families curated via sequence similarity to maize and rice and unique family protein motif searches are presented. A total of 4086 wheat, 1118 maize, 1036 rice, and 955 Arabidopsis genes were aligned and placed into gene family trees to present homologs for all four species. Due to hexaploidy, many wheat cell wall gene families show expected triplication of genes per family over maize, rice, and Arabidopsis. However, several families contained more wheat genes than expected. The utility of this research is demonstrated with an example from a pre-harvest sprouting study to identify specific gene families rather than the less descriptive identification available with standard bioinformatic searches.
Exhaust Emissions from Euro 6 Vehicles in WLTC and RDE—Part 1: Methodology and Similarity Conditions Studies
Authors: Pielecha Jacek , Kurtyka Karolina
Published: November 2023

The article is an attempt to perform an ecological assessment of passenger cars with various types of engines in road emission tests. The main research problem and, at the same time, the goal was to develop a method for determining the exhaust emissions from motor vehicles in real traffic conditions based on results obtained in homologation tests. The tests were carried out on vehicles equipped with gasoline, diesel, and hybrid engines, and the obtained results were analyzed. All of the selected vehicles were of the same class—passenger cars, with a similar curb weight, similar maximum engine power, and in the same emission class (Euro 6d). The authors compared the dynamic parameters of vehicle motion in established emission tests: Worldwide harmonized Light vehicles Test Cycles and Real Driving Emissions. Four procedures were used to analyze and compare the operating conditions of the vehicles in the WLTC and RDE tests, differing in how the phases in the tests were divided as well as having a different methodology for determining the road emissions in the tests. The procedures were as follows: WLTC (where the test was divided and the determination of the road emission of exhaust gases was carried out according to the standard WLTP procedure), RDE (the road test was divided into sections and the exhaust emission was determined according to the standard RDE procedure), WLTC1+2 (the test was divided into phases: 1 + 2, 3, and 4; a combination of phases 1 and 2 corresponding to the urban section of the RDE test), WLTCRDE (where drive phases were divided and emissions determined in the same way as in the RDE procedure, which assumes the division of the test into sections based on vehicle speed). The implementation of the research task in the form of an algorithm procedure when comparing the dynamic parameters of the movement in the WLTC and RDE tests is the leading goal presented in this article. The division of the WLTC test into sections (urban, rural, and motorway) according to the RDE procedure and also the calculation of the total emissions in the test according to this procedure resulted in obtaining similar road emission values in the test.
Design and Optimization of Heat Sinks for the Liquid Cooling of Electronics with Multiple Heat Sources: A Literature Review
Authors: Li Yijun , Roux Stéphane , Castelain Cathy , Fan Yilin , Luo Lingai
Published: November 2023

This paper presents a detailed literature review on the thermal management issue faced by electronic devices, particularly concerning uneven heating and overheating problems. Special focus is given to the design and structural optimization of heat sinks for efficient single-phase liquid cooling. Firstly, the paper highlights the common presence and detrimental consequences of electronics overheating resulting from multiple heat sources, supported by various illustrative examples. Subsequently, the emphasis is placed on single-phase liquid cooling as one of the effective thermal management technologies for power electronics, as well as on the enhancement of heat transfer in micro/mini channel heat sinks. Various studies on the design and structural optimization of heat sinks are then analyzed and categorized into five main areas: (1) optimization of channel cross-section shape, (2) optimization of channel flow passage, (3) flow distribution optimization for parallel straight channel heat sinks, (4) optimization of pin-fin shape and arrangement, and (5) topology optimization of global flow configuration. After presenting a broad and complete overview of the state of the art, the paper concludes with a critical analysis of the methods and results from the literature and highlights the research perspectives and challenges in the field. It is shown that the issue of uneven and overheating caused by multiple heat sources, which is commonly observed in modern electronics, has received less attention in the literature compared to uniform or single-peak heating. While several design and structural optimization techniques have been implemented to enhance the cooling performance of heat sinks, topology optimization has experienced significant advancements in recent years and appears to be the most promising technology due to its highest degree of freedom to treat the uneven heating problem. This paper can serve as an essential reference contributing to the development of liquid-cooling heat sinks for efficient thermal management of electronics.
A Review of Isobutanol as a Fuel for Internal Combustion Engines
Authors: Olson André L. , Tunér Martin , Verhelst Sebastian
Published: November 2023

Isobutanol, one of the four isomers of butanol (C4H9OH), possesses some favorable properties that make it an attractive fuel for internal combustion engines. For instance, when compared to ethanol, isobutanol features a higher heating value and lower hygroscopicity (which prevents corrosion and enables it to be transported via pipelines). Moreover, its addition to gasoline does not distort the fuel blend’s vapor pressure to the same extent as ethanol does. All of this while having a high octane rating. Those advantages over ethanol suggest that isobutanol has the potential to be used as a gasoline oxygenate or even as a neat fuel. Furthermore, the advances made in biotechnology have enabled isobutanol to be produced from biomass more efficiently, allowing it to be used in compliance with existing renewable energy mandates. This article reviews some of the relevant literature dedicated to isobutanol as a motor fuel, covering its merits and drawbacks. Several studies on its combustion characteristics are also discussed. Most of the included literature refers to the use of isobutanol in spark-ignition (SI) engines, as its properties naturally lend themselves to such applications. However, isobutanol’s utilization in diesel engines is also addressed, along with a couple of low-temperature combustion examples.
The Comparative Analysis of Carbon Pricing Policies on Canadian Northwest Territories’ Economy under Different Climate Change Scenarios
Authors: Berthe Abdrahmane , Kubursi Atif , Arain M. Altaf , Janes Ashley
Published: November 2023

Policymakers in the Northwest Territories have introduced carbon pricing as a strategy to reduce fossil fuel consumption and CO2 emissions across various population segments and industries. This indirect approach, chosen for its acceptability, aims to influence behavior rather than directly limit carbon-intensive products. The main purpose of this study was to evaluate the economic and ecological impacts of this policy and its alignment with intended objectives. Using a CGE macroeconomic model incorporating economic structural and behavioral equations, we assessed the policy’s effects on NWT’s economy in general and on a subset of its key sectors. We also incorporated a few observed and simulated climate data for diverse climate change scenarios. The estimated results revealed that climate variables, especially precipitation, significantly influenced sectors like agriculture, construction, and manufacturing. The standardized precipitation evapotranspiration index (SPEI), which encompasses both temperature and precipitation, notably impacted the agriculture, oil, and gas sectors. However, temperature alone showed limited significance, except in the oil and gas sector. The simulation results indicated that, while carbon pricing reduced economic contributions of fossil fuel sector, household rebates could counteract these effects of the economic growth of NWT. Our findings offer valuable insights for shaping NWT’s environmental policies, aligning them with Canada’s goal of net-zero CO2 emissions by 2050.
Model-Driven Membrane Electrode Assembly Design for High-Performing Open-Cathode Polymer Electrolyte Membrane Fuel Cells
Authors: Sagar Anand , Chugh Sachin , Kjeang Erik
Published: November 2023

Open-cathode fuel cells use air cooling to effectively reduce system cost. However, due to the challenging hygrothermal environment, they generally suffer from low performance compared to conventional, liquid-cooled cells. A pre-validated, three-dimensional computational model is used in the present work to determine the effects of different sub-component designs, namely the polymeric membrane, composition of the cathode catalyst layer (CCL), and structure of the cathode microporous layer (CMPL), on the performance of an open-cathode fuel cell. This comprehensive parametric study performed on a total of 90 cases shows the increment in current density to be 7% and 31% by improvising the membrane and CCL design, respectively, at 0.6 V. A steep increase of 87% is also achieved by strategically modifying the CMPL design at 0.4 V operation. An overall increment of 119% and 131% in current density is achieved for the best membrane electrode assembly (MEA) design at 0.6 and 0.4 V, respectively, as compared to the baseline design. These improvements are achieved by collective improvements in kinetics, oxygen mass transport, ohmic resistance, self-heating, and water retention in the ionomer phase. The proposed MEA design could facilitate open-cathode fuel cell stacks with 2× higher power output or 56% lower weight and materials cost for a given power demand.
A Novel Approach for Day-Ahead Hourly Building-Integrated Photovoltaic Power Prediction by Using Feature Engineering and Simple Weather Forecasting Service
Authors: Jeong Jinhwa , Lee Dongkyu , Chae Young Tae
Published: November 2023

Although the accuracy of short-term prediction of building-integrated photovoltaics is essential to making an optimal decision on the management of the generated electricity, the weather forecasting service in many countries provides insufficient features for improving the prediction accuracy of the photovoltaics power output. This study suggests a machine learning model incorporated with feature engineering to improve the prediction performance of day-ahead hourly power outputs using a simple weather forecast service. A new synthetic feature, the modified sky condition, is derived to infer onsite sky condition and solar irradiation, which is not supported by the typical weather forecasting services. It evaluated the prediction performance with different training and hyper-parameter conditions for 60 days. By using the derived modified sky condition, the model outperformed other predictor configurations in most daily sky conditions; particularly, the accuracy improved by more than 50% on overcast days compared to when it used the original weather forecasting service data. The result demonstrates the feasibility and ability of the model to enable more efficient energy management of building-integrated photovoltaic power output in buildings without an onsite weather station, thus contributing toward the optimized dispatch of the integrated electricity energy storage system and other distributed energy resources.
Validation of a Generic Non-Swirled Multi-Fuel Burner for the Measurement of Flame Stability Limits for Research of Advanced Sustainable Aviation Fuels
Authors: Zimmermann Paul , Bajrami Julian , Dinkelacker Friedrich
Published: November 2023

Future aviation concepts should be both CO2-neutral and without other emissions. One approach to reaching both targets is based on sustainably produced synthetic liquid fuels, which may allow very clean, lean premixed prevaporized (LPP) combustion. For that, fuels are needed with much longer ignition delay times and a lower flashback propensity than current jet fuels. We describe an experimental setup to investigate the flashback stability of liquid fuels in a multi-fuel burner. In this work, the measurement procedure and the determination of the experimentally obtained accuracy are in focus with regard to prevaporized and preheated iso-propanol/air flames in an equivalence ratio range of 0.85 to 1.05 involving three preheating levels (573, 673, and 773 K). As the determination of the accurate unburnt gas temperature just ahead of the flame is of strong importance for flashback but not directly possible, a model is implemented to determine it from the measurable quantities. Even with this indirect method, and also regarding the hysteresis of the experimental preheating temperature, it is found that the relevant quantities, namely, measured temperatures, mass flows, and values derived from them, can be determined with accuracy in the range below 1.7%.