bluuebunny/crossref_metadata_2025 huggingface dataset:
Subsistence economy in the South Caucasus during the Early Chalcolithic period: bioarchaeological analysis of Bavra Ablari rock-shelter (Samtskhe-Javakheti region, Georgia)
Authors: Decaix Alexia , Martin Lucie , Messager Erwan , Chahoud Jwana , Varoutsikos Bastien , Mgeladze Ana , Gabunia Manana , Agapishvili Tamar , Chataigner Christine
Published: September 2024

This paper examines the subsistence economy in the South Caucasus during the Early Chalcolithic (c.4700–4300 BC) through bioarchaeological analyses of the Bavra Ablari rock shelter site. This region, rich in biodiversity and characterized by a variety of climates and landscapes, has a history of agropastoral occupation dating back to the beginning of the 6th millennium BC. Up to now, archaeological studies have mainly focused on the valleys and lowlands, leaving the mountainous areas less explored. Recent excavations at Bavra Ablari, located at an altitude of 1,650 m, have enabled new bioarchaeological analyses to be carried out, providing data on the faunal and botanical assemblages of this period. These analyses reveal a mixed agro-pastoral exploitation, with a predominance of caprine (sheep and goats) rearing and cultivation of cereals, such as barley and einkorn. Faunal remains and evidence of hunting and fishing reveal extensive use of several biotopes. The study highlights the importance of pastoralism, attested to as far back as the Neolithic period, with herds moving seasonally to higher pastures in summer. Early Chalcolithic occupations, such as those at Bavra Ablari, show the persistence of pastoral activities in these mountainous regions despite severe winter conditions and suggest seasonal occupation of the site.
Risk assessment of goaf instability based on a variable weight model
Authors: Guo Linning , Hou Kepeng , Sun Huafen , Yang Yong
Published: September 2024

Goaf instability poses significant hazards, affecting mine safety and public welfare. This study aims to evaluate the risk of goaf instability to enhance safety measures in mining operations. Thirteen key indicators were identified to construct a comprehensive evaluation index system. By integrating game theory, we combined subjective and objective weights to develop a constant weight model, which was subsequently improved by considering data distribution characteristics to develop a local variable weight model. The variable weight intervals were determined through cumulative frequency analysis of normalized factor indices, and the Monte Carlo method was employed to define weight adjustment parameters. Using the cloud model, we assessed the instability risk of goafs. Our results indicate that the variable weight model provides higher evaluation accuracy compared to the constant weight model, offering clearer and more distinguishable membership degrees for the evaluation outcomes, suggesting its potential for more precise risk assessments in mining operations.
Addressing colonial and militarized themes in STEM education
Authors: Porter Sean , Siddiqui Yusra
Published: September 2024

In response to longstanding inequities and injustices within STEM, this review addresses the pressing need to decolonize STEM education and redefine the purpose of its disciplinary fields. Focusing on the influence of entrenched power structures, particularly the military-industrial complex, this review examines the relatively under-theorized impact of these forces on shaping the goals and scope of certain bodies of STEM education within UK higher education. The first section offers an overview of militarized STEM education and its connections to the challenges of decolonization. The second section explores strategies and interventions for decolonial pedagogy aimed at challenging discourses and practices that reinforce colonial and militarized narratives within curriculum and teaching. This review highlights how critical pedagogy and Indigenous Knowledge Systems offer educators' methods to cultivate criticality and humanity in their teaching practices. Ultimately, the review attempts to highlight how STEM education can be re-envisioned to serve broader, more emancipatory, and just purposes. Here the review advocates for a transformative educational paradigm that integrates inclusive pedagogical interventions with critical engagement in the ethical and moral dimensions of STEM practice, with the overarching goal of advancing social justice in teaching practices.
Association between serum insulin-like growth factor-1 and bone mineral density in patients with type 2 diabetes
Authors: Zhao Qianqian , Li Youqian , Zhang Qiuping , Zhang Mei , Ban Bo
Published: September 2024

Background

Secondary osteoporosis is associated with type 2 diabetes mellitus (T2DM), and there is conflicting evidence regarding the relationship between insulin-like growth factor-1 (IGF-1) and bone mineral density (BMD) in different populations. The objective of this study was to investigate the relationship between serum IGF-1 levels and BMD in patients with T2DM.

Method

A retrospective cross-sectional study was performed on a cohort of 363 patients with T2DM, comprising men aged over 50 and women who are postmenopausal. Those with no significant medical history or medication affecting BMD or IGF-1 were considered. Data analyzed included IGF-1 levels, markers of bone metabolism, and measurements of BMD. To account for age and gender variations, we calculated IGF-1 standard deviation scores (IGF-1 SDS) for further investigation.

Results

A significant increase in BMD at lumbar spine (LS), femoral neck (FN), and total hip (TH) was observed as IGF-1 SDS tertiles rose. We revealed a nonlinear correlation between IGF-1 SDS and BMD at these sites, with a common inflection point identified at an IGF-1 SDS level of -1.68. Additionally, our multivariate piecewise linear regression analysis highlighted a positive association between IGF-1 SDS and BMD at LS, FN, and TH when IGF-1 SDS exceeded the inflection point (β 0.02, 95% CI 0.01, 0.04 for LS; β 0.02, 95% CI 0.01, 0.03 for FN; β 0.02, 95% CI 0.01, 0.03 for TH). Conversely, below the inflection point, this association was not significant (β -0.04, 95% CI -0.10, 0.01 for LS; β -0.04, 95% CI -0.08, 0.01 for FN; β -0.03, 95% CI -0.08, 0.01 for TH).

Conclusion

These findings reveal a nonlinear relationship between IGF-1 SDS and BMD in T2DM patients. Higher serum IGF-1 levels were connected to increased bone density only after surpassing a certain threshold.
Wind power short-term prediction based on digital twin technology
Authors: Liu Shu
Published: September 2024

Wind power generation has become an indispensable part of the power supply side of the power grid. Due to the intermittent and uncertain characteristics of wind energy, short-term wind power prediction plays an important role in the stable operation of power system. By constructing the digital twin model, real-time and high-precision prediction of wind energy is realized. First, the genetic algorithm-support vector machine (GA-SVM) algorithm is used to build the model. Multidimensional sensors and meteorological stations of the virtual wind power generation system collected the meteorological data of the environment and updated the meteorological history database at the same time. Second, based on the collected meteorological data, the preliminary prediction results are obtained, and by searching in the historical database, the predicted value and the actual output value of wind turbines or wind farms under similar conditions are obtained. Finally, the prediction results of the GA-SVM are modified to obtain the predicted value of the digital twin model. The prediction method can greatly improve the accuracy of the short-term forecast of wind energy.
Comparison of carbon uptake strategies between Chara aspera and Chara tomentosa growing in the brackish Baltic Sea and in the freshwater lakes of Estonia
Authors: Albert Gerli , Pajusalu Liina , Pritchard Daniel W. , Hepburn Christopher D. , Torn Kaire , Paalme Tiina , Põllumäe Arno , Martin Georg
Published: September 2024

The carbon acquisition strategies of aquatic photosynthetic organisms play a key role in the growth and survival of a species. There is much research indicating that the predicted changes (e.g., the balance between carbonate species: CO2, HCO3-, and CO32-) in the seawater carbonate chemistry, due to ocean acidification, could affect benthic primary producers and their communities. However, considerably less is known about brackish water (e.g., the Baltic Sea), and even less about the possible effects of acidification on freshwater biota. This study aimed to compare the carbon uptake strategies of two dominant charophyte species: Chara aspera and Chara tomentosa growing in freshwater lakes of Estonia and in the brackish NE Baltic Sea. This could indicate how they might respond to the predicted increasing CO2 concentration linked to climate change scenrios. Carbon use strategies in charophytes were determined by analysing natural carbon isotope signatures (δ13C), pH drift experiments and photosynthesis vs. dissolved inorganic carbon curves. The study showed that freshwater and brackish water C. aspera and C. tomentosa likely use different carbon uptake mechanisms. Our results indicated that freshwater charophytes preferentially use CO2 and brackish water charophytes HCO3-, likely due to their local acclimatization to different growth environments. Also, C. tomentosa and C. aspera from the studied lakes are likely carbon saturated (photosynthetic processes are operating at their maximum efficiency due to the availability of dissolved inorganic carbon in their environment) and probably will not gain photosynthetic advantages from acidification. However, the predicted increase in CO2 concentration may positively affect the growth of the charophytes in the brackish Baltic Sea.
A case of adrenomyeloneuropathy caused by a novel point mutation in the ABCD1 gene and functional verification
Authors: Shi Xiaoxue , Qi Xuelin , Zheng Jinhua , Ma Jianjun , Li Dongsheng
Published: September 2024

Adrenoleukodystrophy is a rare neurogenetic disease, and adrenomyeloneuropathy is the most common phenotype in adults. The clinical data of a patient with adrenoleukodystrophy and spinal-peripheral neuropathy caused by a novel point mutation in exon 4 of the ABCD1 gene (c.1256T > G (p.Val419Gly)) were retrospectively analyzed. Furthermore, we constructed wild-type and mutant vectors of the ABCD1 (NM0000334) gene to validate the effect of this mutation on the expression of the ABCD1 gene and protein and to explore the mechanism of X-linked adrenoleukodystrophy occurrence and development to identify therapeutic targets for clinical treatment.
Role of disulfidptosis in colorectal adenocarcinoma: implications for prognosis and immunity
Authors: Yang Ruanruan , Lai Chunxiao , Huang Luji , Li Feng , Peng Weiqi , Wu Meiyan , Xin Jinge , Lu Yan , Ouyang Manzhao , Bai Yang , Lei Haoqiang , He Shunhui , Lin Yu
Published: September 2024

Background

Recent research has found a new way of cell death: disulfidptosis. Under glucose starvation, abnormal accumulation of disulfide molecules such as Cystine in Solute Carrier Family 7 Member 11 (SLC7A11) overexpression cells induced disulfide stress to trigger cell death. The research on disulfidptosis is still in its early stages, and its role in the occurrence and development of colorectal malignancies is still unclear.

Method

In this study, we employed bioinformatics methods to analyze the expression and mutation characteristics of disulfidptosis-related genes (DRGs) in colorectal cancer. Consensus clustering analysis was used to identify molecular subtypes of Colorectal Adenocarcinoma (COAD) associated with disulfidptosis. The biological behaviors between subtypes were analyzed to explore the impact of disulfidptosis on the tumor microenvironment. Constructing and validating a prognostic risk model for COAD using diverse data. The influence of key genes on prognosis was evaluated through SHapley Additive exPlanations (SHAP) analysis, and the predictive capability of the model was assessed using Overall Survival analysis, Area Under Curve and risk curves. The immunological status of different patients and the prediction of drug treatment response were determined through immune cell infiltration, TMB, MSI status, and drug sensitivity analysis. Single-cell analysis was employed to explore the expression of genes at the cellular level, and finally validated the expression of key genes in clinical samples.

Result

By integrating the public data from two platforms, we identified 2 colorectal cancer subtypes related to DRGs. Ultimately, we established a prognosis risk model for COAD using 7 genes (FABA4+GIPC2+EGR3+HOXC6+CCL11+CXCL10+ITLN1). SHAP analysis can further explained the positive or negative impact of gene expression on prognosis. By dividing patients into high-risk and low-risk groups, we found that patients in the high-risk group had poorer prognosis, higher TMB, and a higher proportion of MSI-H and MSI-L statuses. We also predicted that drugs such as 5-Fluorouracil, Oxaliplatin, Gefitinib, and Sorafenib would be more effective in low-risk patients, while drugs like Luminesib and Staurosporine would be more effective in high-risk patients. Single-cell analysis revealed that these 7 genes not only differ at the level of immune cells but also in epithelial cells, fibroblasts, and myofibroblasts, among other cell types. Finally, the expression of these key genes was verified in clinical samples, with consistent results.

Conclusions

Our research findings provide evidence for the role of disulfidptosis in COAD and offer new insights for personalized and precise treatment of COAD.
Ferroptosis-associated genes and compounds in renal cell carcinoma
Authors: He Chengwu , Li Qingyi , Wu Weijia , Liu Ke , Li Xingwen , Zheng Hanxiong , Lai Yongchang
Published: September 2024

As the main type of renal cell carcinoma (RCC), clear cell RCC (ccRCC) is often associated with the deletion or mutation of the von Hippel Lindau (VHL) gene, enhancement of glucose and lipid metabolism, and heterogeneity of the tumor microenvironment. VHL alterations in RCC cells lead to the activation of hypoxia-inducible factors and their downstream target vascular endothelial growth factor, and to the reprogramming of multiple cell death pathways and metabolic weakness, including ferroptosis, which are associated with targeted therapy or immunotherapy. The changes in biological metabolites (e.g., iron and lipids) support ferroptosis as a potential therapeutic strategy for RCC, while iron metabolism and ferroptosis regulation have been examined as anti-RCC agents in numerous studies, and various ferroptosis-related molecules have been shown to be related to the metastasis and prognosis of ccRCC. For example, glutathione peroxidase 4 and glutaminase inhibitors can inhibit pyrimidine synthesis and increase reactive oxygen species levels in VHL-deficient RCC cells. In addition, the release of damage-associated molecular patterns by tumor cells undergoing ferroptosis also mediates antitumor immunity, and immune therapy can synergize with targeted therapy or radiotherapy through ferroptosis. However, Inducing ferroptosis not only suppresses cancer, but also promotes cancer development due to its potential negative effects on anti-cancer immunity. Therefore, ferroptosis and various tumor microenviroment-related molecules may co-occur during the development and treatment of RCC, and further understanding of the interactions, core targets, and related drugs of ferroptosis may provide new combination drug strategies for RCC treatment. Here we summarize the key genes and compounds on ferroptosis and RCC in order to envision future treatment strategies and to provide sufficient information for overcoming RCC resistance through ferroptosis.
The pathological significance and potential mechanism of ACLY in cholangiocarcinoma
Authors: Sun Xiaoyan , Zhao Xiaofang , Wang Senyan , Liu Qi , Wei Wenjuan , Xu Jing , Wang Hongyang , Yang Wen
Published: September 2024

Background and aim

Cholangiocarcinoma (CCA) is a rare cancer, yet its incidence and mortality rates have been steadily increasing globally over the past few decades. Currently, there are no effective targeted treatment strategies available for patients. ACLY (ATP Citrate Lyase), a key enzyme in de novo lipogenesis, is aberrantly expressed in several tumors and is associated with malignant progression. However, its role and mechanisms in CCA have not yet been elucidated.

Methods

The expression of ACLY in CCA was assessed using transcriptomic profiles and tissue microarrays. Kaplan-Meier curves were employed to evaluate the prognostic significance of ACLY in CCA. Functional enrichment analysis was used to explore the potential mechanisms of ACLY in CCA. A series of assays were conducted to examine the effects of ACLY on the proliferation and migration of CCA cells. Ferroptosis inducers and inhibitors, along with lipid peroxide probes and MDA assay kits, were utilized to explore the role of ACLY in ferroptosis within CCA. Additionally, lipid-depleted fetal bovine serum and several fatty acids were used to evaluate the impact of fatty acids on ferroptosis induced by ACLY inhibition. Correlation analyses were performed to elucidate the relationship between ACLY and tumor stemness as well as tumor microenvironment.

Results

The expression of ACLY was found to be higher in CCA tissues compared to adjacent normal tissues. Patients with elevated ACLY expression demonstrated poorer overall survival outcomes. ACLY were closed associated with fatty acid metabolism and tumor-initiating cells. Knockdown of ACLY did not significantly impact the proliferation and migration of CCA cells. However, ACLY inhibition led to increased accumulation of lipid peroxides and enhanced sensitivity of CCA cells to ferroptosis inducers. Polyunsaturated fatty acids were observed to inhibit the proliferation of ACLY-knockdown cells; nonetheless, this inhibitory effect was diminished when the cells were cultured in medium supplemented with lipid-depleted fetal bovine serum. Additionally, ACLY expression was negatively correlated with immune cell infiltration and immune scores in CCA.

Conclusion

ACLY promotes ferroptosis by disrupting the balance of saturated and unsaturated fatty acids. ACLY may therefore serve as a potential diagnostic and therapeutic target for CCA.
Rickettsial infections: prevalence and diagnosis of scrub typhus in India
Authors: Ravishankar Vigneshwaran , Narayanan Shridhar , Shandil Radha Krishan
Published: September 2024

Rickettsial infections present a substantial public health burden in India. Recent years have witnessed an increase in the incidence of Rickettsial infection-associated morbidity and mortality. These infections are primarily transmitted by vectors such as ticks, fleas, mites, and lice. This review aims to capture epidemiology, diagnosis and emerging disease trends of rickettsial infections, particularly Orientia tsutsugamushi (O. tsutsugamushi) in the Indian context. Diagnosis and treatment of Orientia infections remain challenging due to the lack of sensitive and specific diagnostic tools vis-a-vis clinical treatment in the absence of specific drugs targeting Rickettsial pathogens. Consequently, clinicians often rely on symptoms and epidemiological factors for diagnosis, highlighting the urgent need for improved diagnostics and therapeutic tools. A comprehensive understanding of the epidemiology of rickettsial diseases is essential for formulating effective preventive and control strategies. Identification of high-risk regions and populations by serological and genetic techniques may help the development of targeted interventions. Therefore, enhancing awareness among healthcare professionals and the public regarding epidemiology, clinical features, diagnosis and treatment interventions of rickettsiosis is crucial. This review summarizes the significance of comprehensive epidemiological investigations and diagnostic systems for understanding rickettsial infections in India.
Improving data-driven estimation of significant wave height through preliminary training on synthetic X-band radar sea clutter imagery
Authors: Rezvov Vadim , Krinitskiy Mikhail , Gavrikov Alexander , Golikov Viktor , Borisov Mikhail , Suslov Alexander , Tilinina Natalia
Published: September 2024

X-band marine radar captures the signal reflected from the sea surface. Theoretical studies indicate that the initial unfiltered signal contains meaningful information about wind wave parameters. Traditional methods of significant wave height (SWH) estimation rely on physical laws describing signal reflection from rough surfaces. However, recent studies suggest the feasibility of employing artificial neural networks (ANNs) for SWH approximation. Both classical and ANN based approaches necessitate costly in situ data. In this study, as a viable alternative, we propose generating synthetic radar images with specified wave parameters using Fourier-based approach and Pierson–Moskowitz wave spectrum. We generate synthetic images and use them for unsupervised learning approach to train a convolutional component of the reconstruction ANN. After that, we train the regression ANN based on the previous convolutional part to obtain SWH back from the synthetic images. Then, we apply preliminary trained weights for the regression model to train SWH approximation on the dataset of real sea clutter images. In this study, we demonstrate the increase in SWH estimation accuracy from radar images with preliminary training on synthetic data.
Study on the three-dimensional numerical simulation of concentrated brine dispersal processes in estuarine bays
Authors: Xue Huaiyuan , Shi Hongyuan , Zhan Chao , Wang Qing , Li Yan , You Zaijin
Published: September 2024

As a new type of liquid discharged into the sea, concentrated brine exhibits complex mixing and stratification patterns due to changes in its density and hydrodynamic properties. This complexity impacts salinity transmission and can result in brine intrusion disasters, directly affecting both ecosystem and human life. This research adopted brine discharge into Dingzi Bay, Shandong Peninsula, a typical estuarine bay, as a case study. 3D convective diffusion numerical simulation techniques were applied to investigate the pathways, salinity rise, impact range, and overall effects on brine dispersal in marine water environments under the combined influence of river flow and tides. The results indicated the followings: (1) Significant spatial variations were observed in flow velocities within Dingzi Bay, with higher velocities near Xiang Island (where river flow turned into the bay) and at bay mouth; (2) The brine discharge point is influenced by complex hydrographic terrain and the combined effects of river flow and tidal movements, resulting in the formation of a high salinity area at the base of the discharge point, where salinity increases by more than 4 PSU within a 100-meter radius.; (3) During high tides, high-concentration brine was clearly transported upstream along the tidal channel, with a salinity increase of ≥3psu covering an area of 5.72×104 m2, extending up to 270 m upstream and 180 m downstream. Brine discharge led to significant mixing of concentrated seawater with seawater within the bay, altering the spatial and temporal distributions of salinity in Dingzi Bay and consequently affecting local sensitive marine species and water environment safety. This study systematically investigated the process of brine discharge into the ocean and its impact range. It was found that discharging brine in areas with higher currents promotes the mixing and dispersion of brine. Additionally, a seasonal discharge plan should be established to avoid discharges during periods of low runoff (such as winter) to minimize negative impacts on aquatic ecosystems and promote the health and diversity of marine ecosystems.
Risk factors for multidrug-resistant tuberculosis: a predictive model study
Authors: Wu Lianpeng , Cai Xiaoxiao , Jiang Xiangao
Published: September 2024

Objective

To investigate the risk factors associated with Multidrug-resistant tuberculosis (MDR-TB) in people with drug-resistant tuberculosis (DR-TB) and develop a predictive model.

Methods

A total of 893 individuals with DR-TB treated at Wenzhou Central Hospital from January 2018 to December 2022 were included in the study after excluding 178 individuals with incomplete clinical and laboratory data, leaving 715 individuals for analysis. Data on demographic information, baseline clinical characteristics, laboratory and imaging results, and clinical diagnosis were collected to identify the risk factors for MDR-TB and establish a predictive model.

Results

Multivariate logistic regression analysis identified residence in rural areas, retreatment of TB, presence of pulmonary cavity, uric acid (UA) ≥ 346 μmol/L and c-reactive protein (CRP) < 37.3 mg/L as independent risk factors for MDR-TB in individuals with DR-TB. A nomogram model was constructed using these five factors to predict the risk of MDR-TB, with an area under the ROC curve (AUC) of 0.758 for the training group and 0.775 for the validation group. Calibration curve analysis showed good agreement between predicted and actual MDR-TB incidence in both groups, and decision curve analysis showed that the nomogram model had a higher rate of clinical net benefit.

Conclusion

This study suggests that residence, types of TB treatment, presence of pulmonary cavity, UA and CRP are associated with MDR-TB occurrence in individuals with DR-TB, and the nomogram model developed in this study shows promising predictive value.
Prevalence of birth before arrival and associated factors among postpartum women in southern Ethiopia: a community-based cross-sectional study
Authors: Amanuel Tsedeke , Desalegn Mitiku , Lukas Kaleegziabher , Yohannes Tadele
Published: September 2024

Background

Birth before arrival (BBA) constitutes a high-risk newborn population with high perinatal morbidity and mortality. In Ethiopia, most studies and health surveys consider only home and hospital deliveries but do not consider deliveries that take place between the house and health facility. The aim of this study was to assess the prevalence of BBA and its associated factors among postpartum women in Lemo woreda, Hadiya Zone, SNNPR, Ethiopia, 2023.

Methods

A community-based cross-sectional study was conducted among postpartum women in Lemo woreda, Hadiya Zone, SNNPR, Ethiopia, from April 5 to May 20, 2023. Three hundred eighty-two postpartum women who gave birth 6 months prior to this study were included. Twelve out of 36 kebeles were selected randomly, and simple random sampling was employed for the selection of participant women. An interviewer-administered questionnaire was used for data collection. A binary logistic regression analysis was computed, and variables with a p value of <0.25 were included in the final multivariable logistic regression analysis. Model fitness was checked via the Hosmer–Lemeshow goodness-of-fit test (x2 = 16.04, p value = 0.250). Statistical significance was declared via odds ratios and 95% confidence intervals at a p value <0.05.

Results

The prevalence of BBA among women who gave birth in the last 6 months preceding this study in the study area was 15.2% (95% CI: 11.8, 19.1%). In the multivariable analysis, the variables associated with birth before arrival in the final model were having no antenatal care (AOR = 2.63; 95% CI: 1.23, 5.63), having a female autonomy status (AOR = 3.32; 95% CI: 1.12, 9.89), not being knowledgeable about labor symptoms (AOR = 2.15; 95% CI: 1.11, 4.18), and having birth preparedness toward the index birth (AOR = 0.13; 95% CI: 0.05, 0.35).

Conclusion

The prevalence of BBA in the study area was unacceptably high. A statistically significant association was observed between birth before arrival and having no antenatal care, dependent women’s autonomy status, being not knowledgeable about labor symptoms, and having birth preparedness toward the index birth.
Perspectives of clinical research on Shen-Shuai-Ning in the treatment of diabetic nephropathy
Authors: Gao Na , Zhang Xi-jun
Published: September 2024

Diabetic nephropathy (DN) is one of the most common chronic complications in patients with diabetes and remains a leading cause of end-stage renal disease. Despite current treatment strategies focusing primarily on blood glucose control and antihypertensive medications, their efficacy is often limited, failing to halt disease progression effectively. However, Shen-Shuai-Ning (SSN), a traditional Chinese medicine compound, has demonstrated significant clinical efficacy in DN treatment in recent years. SSN exerts multifaceted effects, including immunomodulation, attenuation of oxidative stress, and inhibition of fibrosis, leading to improvements in renal function indices and reductions in proteinuria levels, with favorable tolerability. Furthermore, when combined with conventional treatments, SSN exhibits enhanced therapeutic efficacy, providing a comprehensive and effective treatment strategy for DN patients. Future research endeavors should prioritize large-scale, multicenter clinical trials to validate its efficacy and safety across diverse populations, thereby further advancing its integration into clinical practice.
Assessment of corneal epithelial thickness mapping by spectral-domain optical coherence tomography
Authors: Tañá-Rivero Pedro , Orts-Vila Paz , Tañá-Sanz Pedro , Ramos-Alzamora María , Montés-Micó Robert
Published: September 2024

Background

To assess corneal epithelial-thickness (ET) mapping resulting from spectral-domain-optical-coherence-tomography (SD-OCT) by analysing its repeatability and reproducibility and its utility for screening corneal-refractive-surgery (CRS) candidates.

Methods

ET was measured in 25-sectors by two-operators. Intra-subject-standard-deviation, coefficient-of-repeatability (CoR) and coefficient-of-variability (CoV) were calculated to evaluate repeatability. Reproducibility was evaluated using a Bland–Altman analysis. Scheimpflug-tomography, refraction, visual acuity, and patient history were used to make a decision on eligibility for CRS. After this decision, the surgeon was shown the patient’s ET map and was asked to reconsider his analysis. The percentage of screenings that changed after evaluating the ET maps was determined.

Results

Forty-three eyes with normal corneas (CRS-group) and 21 eyes not suitable for CRS (non-CRS-group) were studied. For the CRS-group, CoR ranged from 2.03 (central) to 19.73 μm (outer-inferonasal), with the central-sector showing the highest repeatability (CoV: 1.53–1.80%). For the non-CRS-group, CoR ranged from 3.82 (central-middle-superonasal) to 13.42 μm (middle-inferotemporal), with the inner-superonasal-sector showing the highest repeatability (CoV: 2.86–4.46%). There was no statistically significant difference between operators (p > 0.01). In the CRS-group, the outcomes showed a narrow 95% limits-of-agreement (LoA) for the central-and inner-nasal-sectors (about 4 μm), and wider for the inner-superior, outer-superotemporal and outer-inferonasal (about 10–14 μm). In the non-CRS-group, they were for the outer superonasal (about 4 μm), and for the middle-inferotemporal and outer-temporal (about 10 μm), respectively. Candidacy for CRS changed in 7.82% of patients after evaluation of the ET maps, with all of them screened-out.

Conclusion

The SD-OCT provided repeatable and reproducible corneal ET measurements and may alter candidacy for CRS.

Clinical trial registration

German Clinical Trials Register: https://drks.de/search/en/trial/DRKS00032797, identifier: DRKS00032797.
The characteristics of intestinal microbiota in patients with type 2 diabetes and the correlation with the percentage of T-helper cells
Authors: Yang Fan , Li Jinyan , Wei Longqin , Qin Shenghua , Shi Qingfeng , Lu Siyan , Chu Shuyuan
Published: September 2024

Background

Type 2 diabetes (T2D) is related to intestinal microflora changes and immune inflammation. We aimed to investigate the pattern of intestinal flora-systematic T helper (Th) cell linkage in T2D patients.

Methods

Participants with T2D diagnosed by physicians and healthy controls were enrolled in the study. The Th1, Th2, and Th17 cells from the peripheral blood were assessed by flow cytometry. The feces were collected. The V3–V4 variable region of 16S rRNA was sequenced and analyzed using bioinformatics. Principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) analysis were performed to assess the beta diversity. The linear discriminant analysis (LDA) effect size (LEfSe) method was applied to identify amicrobial taxon specific to T2D. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was conducted to identify the metabolic pathways. A network analysis was conducted by constructing a co-occurrence network.

Results

The percentages of the Th1 and Th17 cells in the peripheral blood were higher in patients with T2D than in controls. Among the top 30 genera of the intestinal microbiota, the levels of Lachnospiraceae_NK4A136_group, Ruminococcaceae_UCG002, and Eubacterium_hallii_group were lower in the patients with T2D than in controls. In the LEfSe analysis, it was observed that the Lachnospiraceae and Ruminococcaceae families were significantly different between patients with T2D and controls. Moreover, the Th1/Th2 ratio was positively correlated with the abundance of the Lachnoclostridium and Ruminococcus_torques_group genera. In the network analysis, the Th1/Th2 ratio, Ruminococcaceae_UCG-002, and Lachnospiraceae_NK4A136_group were the important nodes.

Conclusion

This study provided a preliminary picture of the crosstalk between the intestinal microbiome and systematic Th cells in patients with T2D. The findings of the study suggested that the network relationship among the intestinal microbiota, metabolites, and CD4+T lymphocyte immunity was unbalanced in the patients with T2D, which might have promoted the development of T2D. This presents a therapeutic opportunity to modulate gut immune reaction and then chronic inflammation by manipulating microbiome-specific Th-cell response.
Significance of zinc-solubilizing plant growth-promoting rhizobacterial strains in nutrient acquisition, enhancement of growth, yield, and oil content of canola (Brassica napus L.)
Authors: Jalal-Ud-Din Sabahet , Elahi Nosheen Noor , Mubeen Fathia
Published: September 2024

The present study was conducted with the aim to isolate, characterize, and identify the promising zinc-solubilizing rhizobacteria found naturally in the rhizosphere of canola (Brassica napus L.) plants. The study investigated the roles of these strains in nutrient acquisition and assimilation of extracellular molecules such as hormones and secondary metabolites. Ten isolated promising zinc-solubilizing strains (CLS1, CLS2, CLS3, CLS6, CLS8, CLS9, CLS11, CLS12, CLS13, and CLS15) were selected and characterized biochemically. Almost all the tested strains were Gram-positive, could fix nitrogen, and were positive for indole acetic acid, HCN, exopolysaccharides, and siderophore production. These effective zinc-solubilizing strains were identified through 16S rRNA gene sequencing. Based on the amount of solubilized zinc and halo zone diameter, four potent strains (CLS1, CLS2, CLS3, and CLS9) were selected for pot and field evaluation. Among all the identified bacterial genera isolated from the rhizosphere of the same host plant at different sampling sites, Priestia aryabhattai was found most abundant and found at all three sampling sites. The strains Priestia megaterium, Staphylococcus succinus, and Bacillus cereus were found at two different sites. Bacillus subtilis was found at only one site. These strains have a number of plant growth-stimulating characteristics as well as the ability to colonize plant roots successfully. The results indicated that inoculation of all these four zinc-solubilizing tested strains enhanced the plant growth, oil contents, and yield attributes of canola as compared to non-inoculated control with fertilizer levels. Staphylococcus succinus (CLS1) was first reported as a zinc solubilizer and associated with canola. Priestia aryabhattai (CLS2) and Priestia megaterium (CLS9) were found to be the best strains, with the most pronounced beneficial effect on canola growth and yield traits in both pot and field conditions. The site-specific dominance of these strains observed in this study may contribute toward decision-making for the development of specific inocula for canola. Therefore, identification of these strains could help in providing adequate amount of soluble zinc along with enhanced plant growth, yield, and oil content of canola.
Anti-neuroinflammatory effects of conjugated linoleic acid isomers, c9,t11 and t10,c12, on activated BV-2 microglial cells
Authors: Porcedda Clara , Manca Claudia , Carta Gianfranca , Piras Franca , Banni Sebastiano , Sogos Valeria , Murru Elisabetta
Published: September 2024

Conjugated linoleic acid (CLA) isomers exhibit anti-inflammatory properties within the central nervous system (CNS). This study investigated the effects of CLA isomers c9,t11 and t10,c12 on fatty acid (FA) and N-acylethanolamine (NAE) profiles and their association with pro-inflammatory molecule expression in BV-2 microglia cell line, the CNS's resident immune cells responsible for maintaining neuronal activity and immune homeostasis. BV-2 cells were treated with 25 μM of c9,t11-CLA, t10,c12-CLA, or oleic acid (OA) for 24 h, followed by lipopolysaccharide (LPS) stimulation. After treatment, the cell's FA and NAE profiles and pro-inflammatory molecule expression were analyzed. Our results demonstrated that CLA isomers mitigate LPS-induced morphological changes in BV-2 cells and reduce gene expression and protein levels of inflammatory markers. This effect was linked to an upregulation of acyl-CoA oxidase 1, a key enzyme in the anti-inflammatory peroxisomal beta-oxidation pathway that efficiently metabolizes CLA isomers. Notably, t10,c12-CLA significantly suppressed stearoyl-CoA desaturase 1, impacting monounsaturated fatty acid synthesis. The NAEs profile was remarkably altered by CLA isomers, with a significant release of the anti-neuroinflammatory mediator docosahexaenoic acid (DHA)-derived N-acylethanolamine (DHAEA). In conclusion, our findings suggest that the anti-neuroinflammatory effects of CLA isomers are due to their unique influences on FA metabolism and the modulation of bioactive FA-derived NAEs, highlighting a potential strategy for nutritional intervention in conditions characterized by neuroinflammation.
Walking and scuba diving assisted amphibious exoskeleton robots: the designing of power assist control and myoelectricity based wearers' fatigue evaluation
Authors: Wang Shuai , Yao Yinuo , Lu Xuwei , Qin Pengjie , Wang Xiangyang , Sun Jianquan , Chen Chunjie , Wu Xinyu
Published: September 2024

Exoskeleton robots have the potential to augment human motor capabilities. however, current control strategies often require task-specific control laws tailored for different scenarios, which limits the applicability of exoskeletons. In this study, we propose a control strategy for exoskeleton robots that is adaptable across various scenarios. We employ adaptive oscillators (AO) with feedback control to rapidly estimate the wearer's motion phase and subsequently provide torque assistance to the wearer's hip joint based on a TCN-LSTM model. During experiments, we collected surface electromyographic (sEMG) signals from the tibialis anterior, gastrocnemius, and rectus muscles of seven groups of subjects performing treadmill walking and inclined treadmill exercises. We utilized the short-time Fourier transform to extract frequency characteristics of the signals and statistically analyzed the rate of frequency change in each muscle group under different strategies. The results indicate that when wearing the exoskeleton, the overall muscle frequency changes more slowly, suggesting that subjects can maintain activity for a longer duration before fatigue sets in. This control strategy effectively reduces the energetic cost of lower limb work for the wearer and enhances the exoskeleton's versatility in various applications.
The transformative potential of mRNA vaccines for glioblastoma and human cancer: technological advances and translation to clinical trials
Authors: Tapescu Iulia , Madsen Peter J. , Lowenstein Pedro R. , Castro Maria G. , Bagley Stephen J. , Fan Yi , Brem Steven
Published: September 2024

Originally devised for cancer control, mRNA vaccines have risen to the forefront of medicine as effective instruments for control of infectious disease, notably their pivotal role in combating the COVID-19 pandemic. This review focuses on fundamental aspects of the development of mRNA vaccines, e.g., tumor antigens, vector design, and precise delivery methodologies, – highlighting key technological advances. The recent, promising success of personalized mRNA vaccines against pancreatic cancer and melanoma illustrates the potential value for other intractable, immunologically resistant, solid tumors, such as glioblastoma, as well as the potential for synergies with a combinatorial, immunotherapeutic approach. The impact and progress in human cancer, including pancreatic cancer, head and neck cancer, bladder cancer are reviewed, as are lessons learned from first-in-human CAR-T cell, DNA and dendritic cell vaccines targeting glioblastoma. Going forward, a roadmap is provided for the transformative potential of mRNA vaccines to advance cancer immunotherapy, with a particular focus on the opportunities and challenges of glioblastoma. The current landscape of glioblastoma immunotherapy and gene therapy is reviewed with an eye to combinatorial approaches harnessing RNA science. Preliminary preclinical and clinical data supports the concept that mRNA vaccines could be a viable, novel approach to prolong survival in patients with glioblastoma.
PXDN as a pan-cancer biomarker and promotes tumor progress via immune inhibition in nasopharyngeal carcinoma
Authors: Li Yu-Chun , Xiao Yan-Hong , Chen Fei-Xiong , Xiao Si-Yu , Lin Jian-Mei , Cai Shu-Tao , Zeng Cui-Lan , Ye Xue-Yan , Yu Xu-Fa , Yuan Li , Li Shi-Bing
Published: September 2024

Background

Investigating oncogenes and the mechanisms driving oncogenic processes in human tumors is imperative for the development of efficient therapies. Peroxidasin (PXDN) has been reported to play a critical role in tissue development and homeostasis. However, the role of PXDN in the occurrence and development of Nasopharyngeal Carcinoma (NPC) remains unknown.

Methods

Data from multiple databases, including GEO and TCGA, were used to analyze the expression levels of PXDN. Taking nasopharyngeal carcinoma as an example, in vitro experiments were conducted to explore the biological functions of PXDN. Overexpression of stable cell lines was achieved through lentiviral infection, cell proliferation was examined using CCK8 and BrdU incorporation assays, and clone formation experiments were performed to assess cell growth. Transwell and wound healing assays were employed to evaluate cell invasion and migration abilities. Additionally, immunofluorescence staining with multiple targets was used to analyze the immune microenvironment of the tumor tissues. Co-culture experiments, followed by clone formation and CFSE incorporation assays, were conducted to observe the impact of NPC stable cell lines on T cells. Flow cytometry was performed to detect surface markers and cytokines in T cells after co-culture to assess T cell function.

Results

PXDN was highly expressed in multiple tumors, and its high expression and mutation profile were correlated with poor survival. Functionally, PXDN plays a crucial role in promoting oncogenic processes by enhancing NPC cell proliferation and metastasis. Mechanistically, PXDN activates extracellular matrix (ECM) signaling pathways while simultaneously inhibiting T-cell infiltration and activation, thereby facilitating cancer progression.

Conclusion

We characterized PXDN as a valuable biomarker for pan-cancer diagnosis and prognosis. We also uncovered new oncogenic roles for PXDN in promoting cancer progression and regulating T-cell immunosuppressive function in NPC.
A systematic review and meta-analysis of intraperitoneal anastomosis versus extraperitoneal anastomosis in laparoscopic left colectomy
Authors: Zhou Wenjie , Wang Xueting , Dan Jie , Zhu Mingjie , Li Ming , Liu Ke , Liao Qian , Wang Yonghong
Published: September 2024

Background

The effectiveness of the anastomosis method for laparoscopic left colectomy (LLC) remains inconclusive. Thus, a systematic review and meta-analysis were conducted to compare the outcomes between intraperitoneal anastomosis (IPA) and extraperitoneal anastomosis(EPA)in LLC.

Methods

PubMed, Embase, the Cochrane Library, CNKI, and WanFangData were systematically searched for relevant literature. The literature was screened independently by two groups, and data were extracted and evaluated for bias. Meta-analysis was performed using Revman5.4 software.

Results

Twelve studies with a total of 1,278 patients were included in our meta-analysis. Compared with the EPA group, the IPA group had less blood loss [odds ratio (OR)=–20.32, 95% confidence interval (CI) (−27.98–12.65), p<0.00001], a lower overall complication rate [OR=0.45, 95% CI (0.33–0.63), p<0.00001], fewer non-severe complications [OR=0.44, 95% CI (0.30–0.64), p<0.0001], and fewer surgical site infections [OR=0.39, 95% CI (0.21–0.71), p=0.002]. Additionally, a longer operation time appeared in the multicenter and propensity score matching (PSM) subgroups of the IPA group. Furthermore, patients in the IPA group had an earlier exhaust time and shorter hospital stays. There were no significant differences between the two groups regarding severe complications, anastomose-related complications, postoperative blood transfusion, ileus, reoperation rate, time to stool, pathologic sample length, and lymph node dissection number.

Conclusion

IPA seems more advantageous than EPA for patients receiving LCC in terms of complications and postoperative recovery and has similar oncological outcomes. However, it may take longer and be more difficult to perform.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/#recordDetails PROSPERO, identifier (CRD4202454391).
Cognitive impairment in children with 5q-associated spinal muscular atrophy type 1: two case reports and the review of the literature
Authors: Yang Hua , Yang Jie , Xue Yawen , Liao Lihui , Cai Qianyun , Luo Rong
Published: September 2024

Spinal muscular atrophy (SMA) is an autosomal recessive disease caused by mutations in the survival motor neuron 1 (SMN1) gene on chromosome 5, leading to the degeneration of lower motor neurons. There are few studies on cognitive impairment comorbid with SMA. Here, we report two cases of severe cognitive impairment in Chinese children with SMA type 1, marking the first such reports in this demographic. We propose that severe cognitive dysfunction may be a comorbidity of SMA. Clinicians should consider SMA in patients presenting with severe muscle weakness and atrophy accompanied by cognitive impairments, to avoid misdiagnosis and oversight.