bluuebunny/crossref_metadata_2025 huggingface dataset:
Determination of Forest Structure from Remote Sensing Data for Modeling the Navigation of Rescue Vehicles
Authors: Rybansky Marian
Published: April 2022

One of the primary purposes of forest fire research is to predict crisis situations and, also, to optimize rescue operations during forest fires. The research results presented in this paper provide a model of Cross-Country Mobility (CCM) of fire brigades in forest areas before or during a fire. In order to develop a methodology of rescue vehicle mobility in a wooded area, the structure of a forest must first be determined. We used a Digital Surface Model (DSM) and Digital Elevation Model (DEM) to determine the Canopy Height Model (CHM). DSM and DEM data were scanned by LiDAR. CHM data and field measurements were used for determining the approximate forest structure (tree height, stem diameters, and stem spacing between trees). Due to updating the CHM and determining the above-mentioned forest structure parameters, tree growth equations and vegetation growth curves were used. The approximate forest structure with calculated tree density (stem spacing) was used for modeling vehicle maneuvers between the trees. Stem diameter data were used in cases where it was easier for the vehicle to override the trees rather than maneuver between them. Although the results of this research are dependent on the density and quality of the input LiDAR data, the designed methodology can be used for modeling the optimal paths of rescue vehicles across a wooded area during forest fires.
Two Approaches to Analyze Whether Citizens’ National Identity Is Affected by Country, Age, and Political Orientation—A Fuzzy Eco-Apostle Model
Authors: Indelicato Alessandro , Martín Juan Carlos
Published: April 2022

The study analyzes national identity using the International Social Survey Program (ISSP) database for the waves of 2003 and 2013. First, the Exploratory Factor Analysis (EFA) and the Multigroup Confirmatory Factor Analysis (MGCFA) are used to find the dimensions of the items included in the national identity module. Second, the civic and ethnic dimensions are analyzed through both a fuzzy clustering analysis and an extended apostle model to classify citizens’ national identity as the following: (1) post nationalists; (2) ethnic oriented; (3) civic-oriented; (4) credentialists. Third, the fuzzy eco-extended apostle model is applied to analyze 16 different national identity categories, for which the four pure mentioned categories are further studied. Fourth, the effects of some social characteristics, such as country-year, political orientation-year, and age-year, on the respective pure national Identity categories are studied using two distinct approaches, namely, contingency tables and conditional probability ratios. Results show that citizens tend to be more pure-credentialist than any other category and that social characteristics play a determinant role in explaining each category of citizens’ national identity.
Development and Validation of a Zone Fire Model Embedding Multi-Fuel Combustion
Authors: Porterie Bernard , Pizzo Yannick , Mense Maxime , Sardoy Nicolas , Louiche Julien , Dizet Nina , Porterie Timothé , Pouschat Priscilla
Published: April 2022

This paper presents the development and validation of a two-zone model to predict fire development in a compartment. The model includes the effects of the ceiling jet on the convective heat transfer to enclosure walls and, unlike existing models, a new concept of surrogate fuel molecule (SFM) to model multi-fuel combustion, and a momentum equation to accurately track the displacement of the smoke layer interface over time. The paper presents a series of full-scale fire experiments conducted in the IUSTI fire laboratory, involving different combinations of solid and liquid fuels, and varying the compartment confinement level. The model results are compared to the experimental data. It was found that for all fire scenarios, the experimental trends are well reproduced by the model. The SFM concept predicts oxygen and carbon dioxide concentrations in the extracted smoke to within a few percent of the measurements, which is a good agreement considering the sensitivity of the model to chemical formulas and combustion properties of fuels. Comparison with other measurements, namely average gas and wall temperatures, is also good. For the large fires reported in this study, the impact of the ceiling jet leads to a slight underestimation of wall temperatures, while the model gives conservative estimates for small fires.
2Ws + 1H Systematic Review to (Re)Draw Actors and Challenges of Participation(s): Focus on Cultural Heritage
Authors: Stiti Khaoula , Ben Rajeb Samia
Published: April 2022

The Council of Europe Framework Convention on the Value of Cultural Heritage for Society, better known as the Faro Convention, emphasizes the relevance of participation in cultural heritage and its clear potential benefits. Despite the growing literature on participation in cultural heritage, little research through systematic reviews has been conducted in this field. This paper explores definitions of participation, its actors, and its challenges with a focus on cultural heritage, and it aims to fill this gap by providing a systematic literature review based on PRISMA 2020 guidelines and Okoli guidelines. The results reflect on the definition of participation, the different actors involved, and the challenges facing participation in cultural heritage, based on the interactions of actors. Results further indicate that participation in cultural heritage specifically is in an early stage of adoption and that considerable effort is needed in assessing the adequate methodologies to face the challenges.
Operation Analysis of Cultural Heritage Service Ecosystems: Empirical Study Based on Dihua Street and Guansi Shihdianzih Old Street in Taiwan
Authors: Kuo Shih-Ling , Chen Chun-Liang
Published: April 2022

For urban development worldwide, the revitalisation of cultural heritage and historical buildings is regarded as a strategy for creating jobs, increasing residents’ access to local culture, improving their quality of life, and developing the urban economy. The key factor in the revitalisation of cultural heritage and historical buildings is a strategy for developing the urban economy. Through an exploratory study, this paper examined how the cultural service ecosystems of Dihua Street and Guansi Shihdianzih Old Street are created and operated and how actors develop cultural service ecosystems. By presenting a common value proposition, actors can benefit from interactions through an exchange of services, provide cultural services, develop cultural value, and implement full resource integration and value co-creation, thus promoting the cultural brand communication of historical blocks and the sustainability of cultural services. This study further extended the original functions of cultural heritage and analysed the operation of cultural service ecosystems for cultural heritage. The findings of this study revealed that the organisational operational effectiveness of revitalisation and cultural innovation activities in historic districts provided an innovative approach for sustainable development and the economic revival of historical blocks, which can be used as a reference for the sustainability of local culture and economy. In this perspective, this article provides some useful suggestions for stakeholders and policymakers.
Prostanoid Signaling in Cancers: Expression and Regulation Patterns of Enzymes and Receptors
Authors: Ershov Pavel V. , Yablokov Evgeniy O. , Kaluzhskiy Leonid A. , Mezentsev Yuri V. , Ivanov Alexis S.
Published: April 2022

Cancer-associated disturbance of prostanoid signaling provides an aberrant accumulation of prostanoids. This signaling consists of 19 target genes, encoding metabolic enzymes and G-protein-coupled receptors, and prostanoids (prostacyclin, thromboxane, and prostaglandins E2, F2α, D2, H2). The study addresses the systems biology analysis of target genes in 24 solid tumors using a data mining pipeline. We analyzed differential expression patterns of genes and proteins, promoter methylation status as well as tissue-specific master regulators and microRNAs. Tumor types were clustered into several groups according to gene expression patterns. Target genes were characterized as low mutated in tumors, with the exception of melanoma. We found at least six ubiquitin ligases and eight protein kinases that post-translationally modified the most connected proteins PTGES3 and PTGIS. Models of regulation of PTGIS and PTGIR gene expression in lung and uterine cancers were suggested. For the first time, we found associations between the patient’s overall survival rates with nine multigene transcriptomics signatures in eight tumors. Expression patterns of each of the six target genes have predictive value with respect to cytostatic therapy response. One of the consequences of the study is an assumption of prostanoid-dependent (or independent) tumor phenotypes. Thus, pharmacologic targeting the prostanoid signaling could be a probable additional anticancer strategy.
Clinical and Radiological Outcomes for Guided Implant Placement in Sites Preserved with Bioactive Glass Bone Graft after Tooth Extraction: A Controlled Clinical Trial
Authors: Baskaran Priyanka , Prakash P.S.G. , Appukuttan Devapriya , Mugri Maryam H. , Sayed Mohammed , Subramanian Sangeetha , Al Wadei Mohammed Hussain Dafer , Ahmed Zeeshan Heera , Dewan Harisha , Porwal Amit , Balaji Thodur Madapusi , Varadarajan Saranya , Heboyan Artak , Fernandes Gustavo V. O. , Patil Shankargouda
Published: April 2022

The goal of the study was to evaluate marginal bone loss (MBL) after 1-year implant placement using a guided implant surgical (GIS) protocol in grafted sockets compared to non-grafted sites. We followed a parallel study design with patients divided into two groups: grafted group (Test group, n = 10) and non-grafted group (Control, n = 10). A bioactive glass bone graft was used for grafting. A single edentulous site with a minimum bone height ≥11 mm and bone width ≥6 mm confirmed by cone-beam computerized tomography (CBCT) was chosen for implant placement. Tapered hybrid implants that were sandblasted and acid-etched (HSA) were placed using the GIS protocol and immediately loaded with a provisional prosthesis. MBL and implant survival rates (ISR) were assessed based on standardized radiographs and clinical exams. Patients were followed up for 1-year post-loading. MBL after one year, in the control group, was −0.31 ± 0.11 mm (mesial) and −0.28 ± 0.09 mm (distal); and in the test group was −0.35 ± 0.11 mm (mesial) and −0.33 ± 0.13 mm (distal), with no statistical significance (p > 0.05). ISR was 100% in both groups after one year. ISR was similar between groups and the marginal bone changes were comparable one year after functional loading, without statistical significance, suggesting that bioactive glass permitted adequate bone formation. The GIS protocol avoided raising flaps and provided a better position to place implants, preserving the marginal bone around implants.
The Feasibility and Acceptability of Neurologic Music Therapy in Subacute Neurorehabilitation and Effects on Patient Mood
Authors: Thompson Naomi , Bloska Jodie , Abington Alison , Masterson Amber , Whitten David , Street Alexander
Published: April 2022

Music interventions support functional outcomes, improve mood, and reduce symptoms of depression in neurorehabilitation. Neurologic music therapy (NMT) has been reported as feasible and helpful in stroke rehabilitation but is not commonly part of multidisciplinary services in acute or subacute settings. This study assessed the feasibility and acceptability of delivering NMT one-day-per-week in a subacute neurorehabilitation centre over 15 months. Data were collected on the number of referrals, who referred, sessions offered, attended, and declined, and reasons why. Staff, patients, and their relatives completed questionnaires rating the interventions. Patients completed the Visual Analog Mood Scales (VAMS) pre and post a single session. Forty-nine patients received 318 NMT sessions (83% of sessions offered). NMT was rated as helpful or very helpful as part of the multidisciplinary team (n = 36). The highest ratings were for concentration, arm and hand rehabilitation, and motivation and mood. VAMS scores (n = 24) showed a reduction in ‘confused’ (−8.6, p = 0.035, effect size 0.49) and an increase in ‘happy’ (6.5, p = 0.021, effect size = 0.12) post NMT. The data suggest that a one-day-per-week NMT post in subacute neurorehabilitation was feasible, acceptable, and helpful, supporting patient engagement in rehabilitation exercises, mood, and motivation.
Protonated Chiral 1,2-Diamine Organocatalysts for N-Selective Nitroso Aldol Reaction
Authors: Shim Jae Ho , Lee Ji Yeon , Kim Hyeon Soo , Ha Deok-Chan
Published: April 2022

The introduction of nitrogen to carbonyl groups is considered both challenging and highly desirable by those who work in the field of organic synthesis. In this study, a diphenylethylenediamine-derived catalyst demonstrating N-selectivity was designed using a quantum calculation for the nitroso aldol reaction. The reductive monoalkylation of (R,R)-(+)-1,2-diphenylethylenediamine afforded an organic chiral diamine catalyst in high yield. The expected reaction mechanism for the nitroso aldol reaction was determined, and the product and solvent conditions were optimized through quantum calculations. The calculation results revealed that the enantioselectivity is determined by the hydrogen bond between the alkyl substituent of the chiral diamine and the oxygen of the aromatic aldehyde on the ammonium moiety. The reaction was found to proceed optimally in the presence of 5 mol % catalyst at −10 °C in brine. Using these conditions, an eco-friendly nitroso aldol reaction was performed in which the organic catalyst and cyclohexanone formed enamine. Nitrosobenzene, activated by hydrogen bonding with an ammonium catalyst, was used to minimize the steric hindrance between the catalyst and the reactant, resulting in high enantioselectivity. A nitroso aldol product with high N-selectivity and enantioselectivity (98% ee) was obtained in 95% yield. The catalyst developed in this study provides a less expensive and more environmentally friendly alternative for the nitroso aldol reaction.
Secretory Immunoglobulin A Immunity in Chronic Obstructive Respiratory Diseases
Authors: de Fays Charlotte , Carlier François M. , Gohy Sophie , Pilette Charles
Published: April 2022

Chronic obstructive pulmonary disease (COPD), asthma and cystic fibrosis (CF) are distinct respiratory diseases that share features such as the obstruction of small airways and disease flare-ups that are called exacerbations and are often caused by infections. Along the airway epithelium, immunoglobulin (Ig) A contributes to first line mucosal protection against inhaled particles and pathogens. Dimeric IgA produced by mucosal plasma cells is transported towards the apical pole of airway epithelial cells by the polymeric Ig receptor (pIgR), where it is released as secretory IgA. Secretory IgA mediates immune exclusion and promotes the clearance of pathogens from the airway surface by inhibiting their adherence to the epithelium. In this review, we summarize the current knowledge regarding alterations of the IgA/pIgR system observed in those major obstructive airway diseases and discuss their implication for disease pathogenesis.
Post-Translational Modifications of ATG4B in the Regulation of Autophagy
Authors: Park Na Yeon , Jo Doo Sin , Cho Dong-Hyung
Published: April 2022

Autophagy plays a key role in eliminating and recycling cellular components in response to stress, including starvation. Dysregulation of autophagy is observed in various diseases, including neurodegenerative diseases, cancer, and diabetes. Autophagy is tightly regulated by autophagy-related (ATG) proteins. Autophagy-related 4 (ATG4) is the sole cysteine protease, and four homologs (ATG4A–D) have been identified in mammals. These proteins have two domains: catalytic and short fingers. ATG4 facilitates autophagy by promoting autophagosome maturation through reversible lipidation and delipidation of seven autophagy-related 8 (ATG8) homologs, including microtubule-associated protein 1-light chain 3 (LC3) and GABA type A receptor-associated protein (GABARAP). Each ATG4 homolog shows a preference for a specific ATG8 homolog. Post-translational modifications of ATG4, including phosphorylation/dephosphorylation, O-GlcNAcylation, oxidation, S-nitrosylation, ubiquitination, and proteolytic cleavage, regulate its activity and ATG8 processing, thus modulating its autophagic activity. We reviewed recent advances in our understanding of the effect of post-translational modification on the regulation, activity, and function of ATG4, the main protease that controls autophagy.
The Key Role of Lung Ultrasound in the Diagnosis of a Mature Cystic Teratoma in a Child with Suspected Difficult to Treat Pneumonia: A Case Report
Authors: Iovine Elio , Petrarca Laura , Regina Domenico , Matera Luigi , Mancino Enrica , Di Mattia Greta , Midulla Fabio , Nenna Raffaella
Published: April 2022

To date, the diagnosis of mediastinal teratoma and mediastinal masses relies on the use of chest X-ray and CT. Lung and thoracic ultrasound is becoming increasingly used in the diagnosis and follow-up of many lung and thoracic diseases. Here, we report the case of a mature cystic teratoma in which the performance of lung ultrasound allowed to speed up the diagnostic workup and to provide the indication for the execution of CT of the thorax allowing the diagnosis.
Photocatalytic Inactivation of Bacillus subtilis Spores by Natural Sphalerite with Persulfate under Visible Light Irradiation
Authors: Liu Yan , Liu Zhenni , Liu Dong , Wang Wanjun
Published: April 2022

Bacterial spores are highly resistant to be inactivated by conventional water disinfection methods. In this study, the inactivation efficiency and mechanisms of Bacillus subtitles (B. subtilis) spores by natural sphalerite (NS) with persulfate (PS) under visible light (Vis) irradiation were investigated for the first time. The NS was composed of ZnS doped with trace amounts of metal ions, including As, Fe, Cd, and Mn. The results showed that 7 log of B. subtilis spores could be completely inactivated within 5 h in the Vis/NS/PS photocatalytic system, and the inactivation efficiency was about four and seven times higher than that of the NS/PS system and the Vis/PS system, respectively. The photo-generated electrons are generated by the excitation of NS under the illumination activated PS to form PS radicals (∙SO4−) and hydroxyl radicals (∙OH), which are the main active species for spore inactivation. Mechanism studies further showed that spore inactivation was related to physiological responses, including the increase in intracellular reactive oxygen species, the change of induced antioxidant enzyme activity, and the change of total protein. Furthermore, the dynamic changes of cells during spore inactivation were observed by SEM. These results not only clarify the relationship between the cell physiological stress response and inactivation mechanism of spores, but also reveal the interaction between minerals and PS under Vis, which provides technical methods for the inactivation of bacterial spores in the field of water disinfection.
Predictive Value of Heat-Shock Protein Gene Expression on Severe Neonatal Hypoxic-Ischemic Encephalopathy
Authors: Seo Yu-Mi , Hwang-Bo Seok , Im Soo-Ah , Kim Myungshin , Youn Young-Ah
Published: April 2022

This study aims to evaluate significant gene expression in severe hypoxic ischemic encephalopathy (HIE) in newborns, which can be used as a predictable measure for high-risk HIE infants. The study prospectively recruited 77 inborn near-term or term HIE newborns between January 2018 and December 2020. We measured six different genes within 6 h of life among the HIE infants and compared the gene levels between the mild- and severe-HIE groups. Among these, 64 HIE infants (83.1%) did not receive therapeutic hypothermia (TH) because they were categorized as mild HIE, and the 13 remaining (16.9%) infants were categorized as ≥ moderate-HIE group and received TH. More abnormal MRI findings, seizure, and use of anti-convulsant were more found in the ≥ moderate = HIE group along with longer mechanical ventilation days and hospitalization. Heat-shock protein 70 family 1 A (HSPA1A) and serpin family H member 1 (SERPINH1) genes, which encode heat-shock protein (HSP) 70 and 47, respectively, were significantly elevated in the ≥ moderate-HIE, seizure, and abnormal MRI groups. HSP 70 and 47 were significantly elevated in the severe-HIE group, possibly playing protective roles in inhibiting exacerbated neuroinflammation and maintaining a cellular homeostasis. At 18–24 months, ≥ moderate-HIE group manifested a significant language delay.
The Association of Economic Growth, Foreign Aid, Foreign Direct Investment and Gross Capital Formation in Indonesia: Evidence from the Toda–Yamamoto Approach
Authors: Sijabat Rosdiana
Published: April 2022

This study examines the direction of causality between Gross Domestic Product, foreign aid, foreign direct investment, and gross capital formation in Indonesia from 1970 to 2019, using the augmented Toda–Yamamoto approach with the Granger causality test. Furthermore, this study achieved the unit root test for both variables using the ADF test, which confirmed that the variables studied were cointegrated and had a prolonged equilibrium relationship with GDP, ODA, FDI, and GCA. The Toda–Yamamoto causality test was used to investigate the direction of causality between variables. The results showed a positive one-way causality between ODA and GDP as well as between FDI and GDP. ODA has promoted the expansion of economic and development activities, thereby leading to GDP in Indonesia. However, despite having a long-run relationship, the study failed to prove a causal relationship between ODA and GCA in Indonesia. Therefore, there is a need for more optimal foreign aid management to attract foreign direct investment.
Assuring Social Equity and Improving Income from an Assessment of Government’s Supports in a Pandemic and Migrant Workers’ Integration in Vietnam
Authors: Nguyen Nga , Nguyen Trang
Published: April 2022

Income improvement is the primary expectation when deciding to migrate. However, due to the limited resources and urban facilities, informal sector work leads to an increasing income gap with local workers, migrant workers in big cities are considered the most vulnerable population. When there is no social policy, migrants are even more susceptible to the negative impacts of COVID-19. To identify necessary bases for short-term and long-term intervention to attract workers to return and quickly adapt to the urban life in the economic recovery process, the study surveyed to clarify the assessment of COVID-19 support packages from which the most beneficial are electricity and water exemption and reduction, food support, loan interest reduction, and loan for salary payment. The study also used survey results from two pandemic centers in the southern region to estimate factors and impacts on the workers’ income in terms of integration, the results show that the major significant factors are education, housing, work sector, self-employment, and social insurance. We take notices to enhance workers’ integration to help retain workers by short-term measurements from the support package’s assessments and long-term measurements from the income and integration estimates to attract workers after the pandemic.
Educational Gardens and Climate Change Education: An Analysis of Spanish Preservice Teachers’ Perceptions
Authors: Corrochano Diego , Ferrari Enzo , López-Luengo María Antonia , Ortega-Quevedo Vanessa
Published: April 2022

Educational gardens are powerful outdoor learning environments to address the subject of climate change and foster climate action. Using an online questionnaire, this study examines the influence of the main sociodemographic and academic factors, and the role of connectedness to nature, on the perception of educational gardens as contexts of climate change education (CCE) among Spanish preservice teachers (PSTs). The sample consisted of 889 PSTs enrolled in 9 university campuses of Spain. The statistical analyses performed evidenced that women are more likely to use educational gardens than men and that there is a progressive decrease in the positive perception of PSTs about the usefulness of gardens for CCE as the educational level at which they are being trained increases. Statistics also revealed that the variable connectedness to nature and the rating of the importance of educational gardens in CCE are not significantly related. Nevertheless, the Mann–Whitney U test indicated that PSTs who scored higher on connection to nature wished to broaden their knowledge of sustainable agriculture and, thus, connectedness to nature could be considered a predictor of environmental attitudes, each influencing the other. Based on these findings, recommendations for PSTs’ training in the CCE context are provided.
Drone-Aided Path Planning for Unmanned Ground Vehicle Rapid Traversing Obstacle Area
Authors: Chang Bao Rong , Tsai Hsiu-Fen , Lyu Jyong-Lin
Published: April 2022

Even with visual contact equipment such as cameras, an unmanned ground vehicle (UGV) alone usually takes a lot of time to navigate an unfamiliar area with obstacles. Therefore, this study proposes a fast drone-aided path planning approach to help UGVs traverse an unfamiliar area with obstacles. In this scenario called UAV/UGV mobile collaboration (abbreviated UAGVMC), a UGV initially invokes an unmanned aerial vehicle (UAV) at the scene to take a ground image and send it back to the cloud to proceed with object detection, image recognition, and path planning (abbreviated odirpp). The cloud then sends the UGV a well-planned path map to help traverse an unfamiliar area. This approach uses the one-stage object detection and image recognition algorithm YOLOv4-CSP to quickly and accurately identify obstacles and the New Bidirectional A* (NBA*) algorithm to plan an optimal route avoiding ground objects. Experiments show that the execution time of path planning for each scene is less than 10 s on average. It does not affect the image quality of the path map. It ensures that the user can correctly interpret the path map and remotely drive the UGV rapidly, passing through that unfamiliar area with obstacles. As a result, the selected model can outperform the other alternatives significantly by average performance ratio up to 3.87 times on average.
An Update on Genetics of Adrenal Gland and Associated Disorders
Authors: Gauss Chester , Rowland Dustin , Ergun-Longmire Berrin
Published: April 2022

The intricacies of human adrenal development have been under scrutiny for decades. Each year marks the identification of new genes and new interactions between gene products that ultimately will act to produce the fully functioning adult gland. Due to the complexity of this process, genetic missteps may lead to a constellation of pathologies. Recent years have identified several novel genetic causes of adrenal dysgenesis and provided new insights into previously delineated processes. SF1, DAX1 (NR0B1), CDKN1C, SAMD9, GLI3, TPIT, MC2R, MRAP, NNT, TXNRD2, AAAS, and MCM4 are among the genes which have had significant contributions to our understanding of the development and function of both adrenals and gonads. Collection and elucidation of these genetic and clinical insights are valuable tools for clinicians who diagnose and manage cases of adrenal dysfunction.
Green Extraction Technology of Polyphenols from Food By-Products
Authors: Kyriakoudi Anastasia , Mourtzinos Ioannis
Published: April 2022

The development of environmentally friendly approaches to produce high-added value compounds is a field of research that has attracted the interest of the scientific community and several industries such as the food and cosmetic industry [...]
Microencapsulation of Cymbopogon citratus D.C. Stapf Essential Oil with Spray Drying: Development, Characterization, and Antioxidant and Antibacterial Activities
Authors: de Oliveira Alencar Denise Dantas , de Souza Evandro Leite , da Cruz Almeida Erika Thayse , da Silva André Leandro , Oliveira Hugo Miguel Lisboa , Cavalcanti Mônica Tejo
Published: April 2022

This study aimed to microencapsulate Cymbopogon citratus essential oil (CCEO) with spray drying using maltodextrin and gelatin. The effects of the operational conditions (inlet temperature (130–160 °C), CCEO concentration (5–15%), maltodextrin concentration (10–20%)) on the physicochemical stability and antioxidant and antibacterial activities of the CCEO microcapsules were determined. The CCEO microencapsulation process had yield and encapsulation efficiency values varying from 31.02 to 77.53% and 15.86–61.95%, respectively. CCEO microcapsules had antibacterial effects against Staphylococcus aureus and Escherichia coli with minimum inhibitory concentration varying from 10 to 20%, and total phenolic contents and antioxidant activities varying from 1632 to 4171.08 μg TE/g and 28.55–45.12 µg/g, respectively. CCEO microcapsules had average diameters varying from 5.10 to 10.11 µm, with spherical external structures without cracks and apparent pores. The best desirable process conditions for CCEO microencapsulation were process inlet temperature of 148 °C, maltodextrin concentration of 15%, and CCEO concentration of 10%. The results showed that CCEO microcapsules with increased stability and low degradation of active components can be prepared by spray drying using maltodextrin and gelatin with the production of microcapsules, which could be exploited as potential food preservatives.
A Bayesian Approach to Describe and Simulate the pH Evolution of Fresh Meat Products Depending on the Preservation Conditions
Authors: Luong Ngoc-Du Martin , Coroller Louis , Zagorec Monique , Moriceau Nicolas , Anthoine Valérie , Guillou Sandrine , Membré Jeanne-Marie
Published: April 2022

Measuring the pH of meat products during storage represents an efficient way to monitor microbial spoilage, since pH is often linked to the growth of several spoilage-associated microorganisms under different conditions. The present work aimed to develop a modelling approach to describe and simulate the pH evolution of fresh meat products, depending on the preservation conditions. The measurement of pH on fresh poultry sausages, made with several lactate formulations and packed under three modified atmospheres (MAP), from several industrial production batches, was used as case-study. A hierarchical Bayesian approach was developed to better adjust kinetic models while handling a low number of measurement points. The pH changes were described as a two-phase evolution, with a first decreasing phase followed by a stabilisation phase. This stabilisation likely took place around the 13th day of storage, under all the considered lactate and MAP conditions. The effects of lactate and MAP on pH previously observed were confirmed herein: (i) lactate addition notably slowed down acidification, regardless of the packaging, whereas (ii) the 50%CO2-50%N2 MAP accelerated the acidification phase. The Bayesian modelling workflow—and the script—could be used for further model adaptation for the pH of other food products and/or other preservation strategies.
Content of Health-Promoting Fatty Acids in Commercial Sheep, Cow and Goat Cheeses
Authors: Szterk Arkadiusz , Ofiara Karol , Strus Bartosz , Abdullaev Ilkhom , Ferenc Karolina , Sady Maria , Flis Sylwia , Gajewski Zdzisław
Published: April 2022

The study aimed to examine samples of different market original sheep cow and goat cheeses, in respect of the content and profile of FA with special emphasis on health-promoting FA. The content of fatty acids in the examined cheeses was highly differentiated and depended on the sort and type of cheese. The content of fatty acid groups in milk fat varied within the limits: SFA, 55.2–67.2%; SCSFA, 10.9–23.4%; BCFA, 1.6–2.9%; MUFA, 15.2–23.4%; PUFA, 1.9–4.3%; trans-MUFA, 1.8–6.0%; and CLA, 1.0–3.1%. From among the examined cheeses, the seasonal sheep cheeses (Oscypek) and mountain cow cheeses were characterized by the highest content of health-promoting fatty acids. The content of health-promoting fatty acids in the fat fraction of these cheeses was CLA 2.1–3.1%, trans-MUFA 3.5–6%, BCFA 2.7–2.9%, and SCSFA 12–18%.
Anisakicidal Effects of R (+) Limonene: An Alternative to Freezing Treatment in the Industrial Anchovy Marinating Process
Authors: Nalbone Luca , Panebianco Felice , Cammilleri Gaetano , Ferrantelli Vincenzo , Giarratana Filippo
Published: April 2022

Anisakiasis is a fish-borne zoonotic disease caused by the ingestion of raw/undercooked fishes or cephalopods parasitized by members of the genus Anisakis. Freezing ensures the inactivation of viable Anisakis larvae; however, since it affects the organoleptic properties of food, essential oils and their compounds were proposed as an alternative. In this study, fresh anchovy fillets were experimentally parasitized with L3 Anisakis larvae to test the anisakicidal efficacy of R (+) limonene (LMN) in marinated fishery products. The anisakicidal effectiveness and organoleptic influence of several LMN concentrations (0.5%, 1%, and 5%) were tested during the marinating process (MS) and storage in sunflower seed oil (SO) of marinated anchovy fillets. Double treatment (DT) with 1% LMN was also performed both during marination and subsequent storage in oil. MS treatment resulted only in a reduction in larvae viability after 48 h, while a complete inactivation was observed in SO after 8, 10, and 20 days of treatment with 5%, 1%, and 0.5% LMN, respectively. DT was the most effective with complete larval inactivation after 7 days. Only 5% LMN influenced the sensory characteristics of the fillets, resulting, however, in a pleasant lemon-like odor and taste. Considering the results obtained, LMN might be a suitable natural alternative to manage Anisakis risk in the fishery industry.
Dynamics and Determinants of the Grain Yield Gap in Major Grain-Producing Areas: A Case Study in Hunan Province, China
Authors: Yu De , Hu Shougeng , Tong Luyi , Xia Cong , Ran Penglai
Published: April 2022

Understanding the dynamics of the grain yield gap (YGAP) and its causative factors is essential for optimizing the layout of grain production and addressing the food crisis, especially in countries with a huge population and less cultivated land, such as China. In the study, a spatial analysis- and machine learning-based framework for YGAP analysis was developed, taking Hunan Province, China, as an application. The results showed that the average YGAP in Hunan Province gradually narrowed from 1990 to 2018, and the YGAPs narrowed in 116 counties. Of which, 26 counties narrowed by more than 4 t ha−1, 58 counties narrowed from 2–4 t ha−1, and 32 counties narrowed within 2 t ha−1. Additionally, we found that the GDP per capita (GDPPC), sunshine hours (SH), per capita annual net income of farmers (PCAI), and rural electricity consumption (REC) play a key role in YGAP change, and the importance of human investment to the YGAP decreased, while socioeconomic environment became the dominant factor that influenced grain production. Comprehensively, the relatively great potential for grain yield growth was generated in sixty-four counties, which are mainly located in the northern, central, and southern Hunan. The findings suggest that it is necessary to consider the trends of economic development in rural areas and population migration in agricultural management. This work provides insights into yield gap dynamics and may contribute to sustainable agricultural management in Hunan Province, China, and other similar regions.
Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment
Authors: Pang Yaru , Wu Ruobin , Cui Tianlin , Zhang Zequn , Dong Li , Chen Fang , Hu Xiaosong
Published: April 2022

In this study, a method of Bacillus subtilis spore inactivation under high pressure (P, 200 MPa) combined with moderate temperature (T, 80 °C) and the addition of antimicrobial peptide LK (102 μg/mL) was investigated. Spores presented cortex hydrolysis and inner membrane (IM) damage with an 8.16 log reduction in response to treatment with PT-LK, as observed by phase-contrast and inverted fluorescence microscopy and flow cytometry (FCM) analysis. Furthermore, a tandem mass tag (TMT) quantitative proteomics approach was utilized because Liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis data were used. After treatment with PT-LK, 17,017 polypeptides and 3166 proteins were detected from B. subtilis spores. Among them, 78 proteins showed significant differences in abundance between the PT-LK-treated and control groups, with 49 proteins being upregulated and 29 proteins being downregulated in the PT-LK-treated group. Genetic information processing, metabolism, cellular process, and environmental information processing were the main mechanisms of PT-LK-mediated spore inactivation.
Animal Species Authentication in Dairy Products
Authors: Mafra Isabel , Honrado Mónica , Amaral Joana S.
Published: April 2022

Milk is one of the most important nutritious foods, widely consumed worldwide, either in its natural form or via dairy products. Currently, several economic, health and ethical issues emphasize the need for a more frequent and rigorous quality control of dairy products and the importance of detecting adulterations in these products. For this reason, several conventional and advanced techniques have been proposed, aiming at detecting and quantifying eventual adulterations, preferentially in a rapid, cost-effective, easy to implement, sensitive and specific way. They have relied mostly on electrophoretic, chromatographic and immunoenzymatic techniques. More recently, mass spectrometry, spectroscopic methods (near infrared (NIR), mid infrared (MIR), nuclear magnetic resonance (NMR) and front face fluorescence coupled to chemometrics), DNA analysis (real-time PCR, high-resolution melting analysis, next generation sequencing and droplet digital PCR) and biosensors have been advanced as innovative tools for dairy product authentication. Milk substitution from high-valued species with lower-cost bovine milk is one of the most frequent adulteration practices. Therefore, this review intends to describe the most relevant developments regarding the current and advanced analytical methodologies applied to species authentication of milk and dairy products.
A Comprehensive Assessment on the Pivotal Role of Hydrogels in Scaffold-Based Bioprinting
Authors: Parimala Chelvi Ratnamani Matangi , Zhang Xinping , Wang Hongjun
Published: April 2022

The past a few decades have seen exponential growth in the field of regenerative medicine. What began as extirpative (complete tissue or organ removal), with little regard to the effects of tissue loss and/or disfigurement, has evolved towards fabricating engineered tissues using personalized living cells (e.g., stem cells), and customizing a matrix or structural organization to support and guide tissue development. Biofabrication, largely accomplished through three-dimensional (3D) printing technology, provides precise, controlled, and layered assemblies of cells and biomaterials, emulating the heterogenous microenvironment of the in vivo tissue architecture. This review provides a concise framework for the bio-manufacturing process and addresses the contributions of hydrogels to biological modeling. The versatility of hydrogels in bioprinting is detailed along with an extensive elaboration of their physical, mechanical, and biological properties, as well as their assets and limitations in bioprinting. The scope of various hydrogels in tissue formation has been discussed through the case studies of biofabricated 3D constructs in order to provide the readers with a glimpse into the barrier-breaking accomplishments of biomedical sciences. In the end, the restraints of bioprinting itself are discussed, accompanied with the identification of available engineering strategies to overcome them.
Chitosan-Based Polyelectrolyte Complex Cryogels with Elasticity, Toughness and Delivery of Curcumin Engineered by Polyions Pair and Cryostructuration Steps
Authors: Dragan Ecaterina Stela , Dinu Maria Valentina , Ghiorghita Claudiu Augustin
Published: April 2022

Chitosan (CS)-based drug delivery systems (DDSs) are often stabilized by chemical cross-linking. A much more friendly approach to deliver drugs in a controlled manner is represented by polyelectrolyte complexes (PECs) physically stabilized by spontaneous interactions between CS and natural or synthetic biocompatible polyanions. PECs with tunable structures, morphologies, and mechanical properties were fabricated in this paper by an innovative and sustainable strategy. Carboxymethyl cellulose (CMC) or poly(2-acrylamido-2-methylpropanesulfonate sodium salt) were used as aqueous solutions, while CS microparticles were evenly dispersed in the polyanion solution, at pH 6.5, where CS was not soluble. Cryostructuration of the dispersion in two steps (5 min at −196 °C, and 24 h at −18 °C), and freeze-drying at −55 °C, 48 h, conducted to pre-PEC cryogels. Next step was rearrangement of complementary polyions and the complex formation inside the pore walls of cryogels by exposure of the pre-PECs at a source of H+. PEC cryogels with impressive elasticity and toughness were engineered in this study by multiple-cryostructuration steps using CMC as polyanion with a molar mass of 250 kDa and an optimum concentration of polyanion and polycation. The performances of PEC cryogels in sustained delivery of anti-inflammatory drugs such as curcumin were demonstrated.
How Did the COVID-19 Pandemic Affect the Use of Emergency Medical Services by Patients Experiencing Mental Health Crises?
Authors: Park Song-Yi , Kim Sun-Hyu
Published: April 2022

The COVID-19 pandemic and its resulting social restrictions have significant implications for mental health. The objective of this study was to determine the monthly trends and types of patients experiencing a mental health crisis (MHC) who used emergency medical services (EMSs) before and during the COVID-19 pandemic. A retrospective observational study was conducted using EMS data. During the study period, 8577 patients used EMSs for MHCs. EMS dispatches for MHCs and suicide completion after the COVID-19 pandemic were decreased by 12.4% and 12.7%, respectively, compared to those before the COVID-19 pandemic. Segmented regression analysis found that the number of patients per month was 6.79 before the COVID-19 pandemic. The number decreased to 4.52 patients per month during the COVID-19 pandemic, although the decrease was not statistically significant. The monthly number of patients experiencing an MHC decreased during strict social distancing measures but increased during relaxed social distancing measures. The percentage of hanging increased from 14.20% before the COVID-19 pandemic to 14.30% (p = 0.03) during the COVID-19 pandemic, whereas the percentages of jumping (from 15.55% to 15.28%, p = 0.01) and self-harm by smoke (from 4.59% to 3.84%, p < 0.001) during the COVID-19 pandemic were decreased compared to those before COVID-19. However, the effect size for the above findings was small (below 0.20). More than 25% of the patients experiencing an MHC who used EMSs refused to transfer to the ED over both study periods (26.49% in the pre-COVID-19 period and 28.53% in the COVID-19 period). The COVID-19 pandemic and social restrictions seemed to have some effects on the use of EMSs by MHC patients. Hanging is mainly performed indoors and is not found easily if social distancing persists, and a patient experiencing an MHC who refuses to be transferred could potentially attempt suicide. Subsequent studies should be performed to determine whether these findings are temporary during the COVID-19 pandemic or whether they will show different aspects after the COVID-19 pandemic.
Medical Students’ Perceptions towards Digitization and Artificial Intelligence: A Mixed-Methods Study
Authors: Gillissen Adrian , Kochanek Tonja , Zupanic Michaela , Ehlers Jan
Published: April 2022

Digital technologies in health care, including artificial intelligence (AI) and robotics, constantly increase. The aim of this study was to explore attitudes of 2020 medical students’ generation towards various aspects of eHealth technologies with the focus on AI using an exploratory sequential mixed-method analysis. Data from semi-structured interviews with 28 students from five medical faculties were used to construct an online survey send to about 80,000 medical students in Germany. Most students expressed positive attitudes towards digital applications in medicine. Students with a problem-based curriculum (PBC) in contrast to those with a science-based curriculum (SBC) and male undergraduate students think that AI solutions result in better diagnosis than those from physicians (p < 0.001). Male undergraduate students had the most positive view of AI (p < 0.002). Around 38% of the students felt ill-prepared and could not answer AI-related questions because digitization in medicine and AI are not a formal part of the medical curriculum. AI rating regarding the usefulness in diagnostics differed significantly between groups. Higher emphasis in medical curriculum of digital solutions in patient care is postulated.
Prompt Hematological Recovery in Response to a Combination of Pegylated Interferon α-2a and Rituximab in a Profoundly Immuno-Suppressed Hairy Cell Leukemia Patient with a Mycobacterial Infection at Onset: Benefits and Drawbacks of Rapid Immune Reconstitution
Authors: Furlan Anna , Rossi Maria Cristina , Gherlinzoni Filippo , Scotton Piergiorgio
Published: April 2022

The present paper reports, to the best of our knowledge for the first time, the efficacy and tolerability of the combination of interferon (IFN)α-2a in pegylated formulation and rituximab after a “priming” phase with IFN in the frontline treatment of hairy cell leukemia (HCL) in a profoundly immunosuppressed patient with a Mycobacterium abscessus infection at onset. This immunotherapy combination may represent a potential therapeutic option in patients with active severe infection and for whom the use of purine nucleoside analogues (PNA) is contraindicated. The benefits and drawbacks of remarkably rapid immune reconstitution in the context of opportunistic infections are highlighted as well, as the potentially paradoxical effects of immune recovery as a result of effective immunotherapy strategies, known as immune reconstitution inflammatory syndrome (IRIS), have to be taken into account when dealing with patients with opportunistic infections.
Production, Composition, and Ecological Function of Sweet-Basil-Seed Mucilage during Hydration
Authors: Zhou Dongfang , Barney Jacob N. , Welbaum Gregory E.
Published: April 2022

The sweet-basil (Ocimum basilicum L.) fruit/pericarp produces mucilage that engulfs the fruit and seed within minutes of hydration. Seed mucilage is produced by plant species that have adapted to arid, sandy soils. This study was conducted to determine how basil-seed mucilage improves ecological fitness. A second objective was to find ways to remove mucilage, which may interfere with commercial planting. Basil fruit/seeds were examined using light and environmental scanning electron microscopy. Columnar structures of basil mucilage rapidly unfolded from the pericarp upon initial hydration. Dilute hydrochloric acid removed the mucilage, which decreased the water content four-fold but did not inhibit seed germination in a laboratory test. Nondestructive Fourier-transform mid-infrared (FTIR) spectroscopy confirmed that the mucilage was primarily composed of hemicellulose that anchored the basil seed to resist movement. The fully hydrated seeds approached zero water potential, so the mucilage did not interfere with hydration. The seeds that were planted in growing media with mucilage had from 12 to 28% higher seedling emergence and survival percentages after 10 days than seeds without mucilage. Basil-fruit/seed mucilage provides a reservoir of loosely bound water at high water potential for seed germination and early seedling development, thus improving survivability under low moisture.