bluuebunny/crossref_metadata_2025 huggingface dataset:
Delayed postoperative spinal epidural hematoma after anterior cervical discectomy and fusion: A case report
Authors: Xu Wenbin , Guo Jiandong , Zhu Jinjin , Zhao Xing , Yasaman Iranmanesh , Chen Jian , Wang Jiying , Fan Shunwu , Fang Xiangqian
Published: September 2022

Background

Postoperative spinal epidural hematoma (POSEH) causes rapid neurological deficits within 24 h following the operation and can be fatal. However, some POSEH symptoms manifest three days after the operation, also known as delayed POSEH (DPOSEH). Little attention has been provided upon DPOSEH owing to its rare incidence, resulting in serious consequences upon occurrence. To date, no cases of delayed POSEH after anterior cervical surgery have been reported.

Case presentation

We describe a case of DPOSEH that presented with delayed neurological deficits on the fifth day after anterior cervical discectomy and fusion (ACDF) surgery. Methylprednisolone was administered but showed no efficacy. MR revealed low T1 and strip long T2 signals located behind discs. After emergency surgical decompression, the patient's muscle strength returned to the preoperative state. However, his muscle strength decreased again on the seventh postoperative day, and the patient's family refused further surgery. Nine months after ACDF, the patient died of septic shock and respiratory failure.

Conclusions

DPOSEH can occur after three days or more following anterior cervical surgery; hence, monitoring of neurological function is suggested to be extended. Complete evaluation of risk factors, timely recognition, and differentiation of neurological symptoms are required for spine surgery. In the case of DPOSEH, methylprednisolone can be administered reasonably during the transition period. However, if there is no resolution of symptoms, emergency surgery should be performed as soon as possible.
Improvement of brain perfusion in patients with chronic brain ischemia at epidural spinal cord electrical stimulation
Authors: Zhao Shu , Sufianova Galina , Shapkin Andrey , Mashkin Andrey , Meshcheryakova Svetlana , Han Dayong
Published: September 2022

Objective

Increasing life expectancy and aging of the population is accompanied by a steady increase in the number of elderly patients with chronic cerebral ischemia and age-related cognitive impairment associated with cerebral hypoperfusion and microangiopathy. The aim of this study was to identify long-term changes in cerebral blood flow (CBF) in patients with chronic cerebral ischemia at the epidural electrical stimulation of the spinal cord (SCS).

Materials and methods

Changes in cerebral blood flow were studied according to CT perfusion in 59 patients (aged 55–78 years) with vertebrogenic pain syndromes and chronic cerebral ischemia during epidural electrical stimulation of the spinal cord at the cervical (C3–C5) and lower thoracic (Th9–Th10) levels.

Results

In all patients, on the 5th day of trial SCS, an increase in cerebral blood flow by from 58.6 ± 1.13 ml/100 ml/min to 64.8 ± 1.21 ml/100 ml/min (p < 0.01) with stimulation at the Th9-Th10 level and from 58.8 ± 1.12 ml/100 ml/min to 68.2 ± 1.42 ml/100 ml/min (p < 0, 01) with stimulation at the C3-C5 level. These changes in brain perfusion were preserved during the follow-up examination 1 year after the implantation of chronic SCS system. The greatest increase in CBF was registered in the frontotemporal regions, subcortical structures and white matter of the brain. Changes in cerebral perfusion did not correlate with the degree of reduction in the severity of the accompanying pain syndrome. The change in CBF in the control group (32 patients) in all periods was not statistically significant.

Conclusion

Our results show that SCS is accompanied by a persistent improvement in brain perfusion, which may be potentially useful for developing methods for reducing age-related vascular disorders in the elderly.
Assessment of Carotid Body Tumors by Superb Microvascular Imaging of Feeding Arteries During Preoperative Evaluation
Authors: Gao Luying , Zhang Xiaoyan , Jiang Yuxin , Wang Hongyan , Zheng Yuehong , Li Wanying , Li Jianchu , Zhang Bo
Published: September 2022

Purpose:

Superb microvascular imaging (SMI) has led to new advances in vascular imaging applications. This study aimed to explore the blood supply and feeding arteries of carotid body tumors (CBTs) on SMI to improve the accuracy of information available to surgeons.

Methods

Twenty-six CBT lesions were subjected to color Doppler flow imaging (CDFI) and SMI and were later confirmed by pathology. The blood flow patterns and feeding arteries of the CBTs on CDFI and SMI were graded and compared.

Results

The feeding arteries of two CBT lesions, which were not visible on CDFI, were identified as the internal carotid artery (ICA) on SMI. The feeding arteries of three CBTs were judged to stem from both the ICA and the external carotid artery (ECA) (MIX) based on SMI compared to the ICA or ECA on CDFI. We classified the feeding arteries of CBTs as originating from the ICA or others (including the ECA and MIX). One hundred percent (3/3) of the CBT lesions stemming from the ICA had Adler I or Adler II blood flow patterns, and 100% (23/23) of the CBT lesions stemming from other arteries had Adler II or Adler III blood flow patterns. Higher Adler categories were assigned based on SMI than CDFI (P < 0.001).

Conclusion

SMI may be superior to CDFI in detecting the vascularity of CBTs, and SMI revealed more potential feeding arteries of CBTs than CDFI. CBTs originating from the ICA are less vascular than those originating from the ECA.
CCDC25 may be a potential diagnostic and prognostic marker of hepatocellular carcinoma: Results from microarray analysis
Authors: Deng Hongyang , Zhang Jiaxing , Zheng Yijun , Li Jipin , Xiao Qi , Wei Fengxian , Han Wei , Xu Xiaodong , Zhang Youcheng
Published: September 2022

Background

Hepatocellular carcinoma (HCC) is a tumor with a high recurrence rate, poor prognosis, and rapid progression. Therefore, it is necessary to find a novel biomarker for HCC. Coiled-coil domain containing 25 (CCDC25) has been identified as a target molecule that mediates liver metastasis in colon cancer. However, the molecular mechanisms of CCDC25 in HCC are unknown. This study aimed to explore the role of CCDC25 in HCC.

Methods

The expression of CCDC25 in HCC was identified through The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Receiver operating characteristic curve (ROC) curves were drawn to evaluate the diagnostic value of CCDC25 for HCC. The effect of CCDC25 on the prognosis of HCC was analyzed by using the Kaplan–Meier plotter. Co-expressed genes and Gene Set Enrichment Analysis (GSEA) were used to explore the related functions and regulatory signaling pathways of CCDC25. Moreover, we employed the Tumor Immune Estimation Resource (TIMER) database and CIBERSORT algorithm to investigate the relationship between CCDC25 and the tumor immune microenvironment (TME) in HCC. Meanwhile, the effect of CCDC25 on the sensitivity of HCC patients to chemotherapy drugs was evaluated. Finally, we explored the prognostic methylation sites of CCDC25 using the MethSurv database.

Results

CCDC25 expression was low in HCC. Low CCDC25 expression was significantly associated with poor overall survival of HCC and may be comparable to the ability of AFP to diagnose HCC. Dysregulation of glucose metabolism, fatty acid metabolism, amino acid metabolism, ubiquitination modification, and apoptosis inhibition caused by CCDC25 downregulation may be the causes and results of HCC. In addition, CCDC25 was positively correlated with the infiltration level of various adaptive antitumor immune cells. The levels of immune cell infiltration and immune checkpoint expression were lower in the samples with high CCDC25 expression. What is more, we found that downregulated CCDC25 may increase the sensitivity or resistance of HCC patients to multiple drugs, including sorafenib. We also identified a methylation site for CCDC25, which may be responsible for poor prognosis and low CCDC25 expression in HCC patients. Finally, CCDC25 may be associated with HCC ferroptosis.

Conclusions

CCDC25 may be a potential diagnostic and prognostic marker for HCC and is associated with immune infiltration and ferroptosis.
Prediction of factors influencing the timing and prognosis of early tracheostomy in patients with multiple rib fractures: A propensity score matching analysis
Authors: Zhang Bing , Li Gong-Ke , Wang Yu-Rong , Wu Fei , Shi Su-Qin , Hang Xin , Feng Qin-Ling , Li Yong , Wan Xian-Yao
Published: September 2022

Objective

To investigate the factors affecting the timing and prognosis of early tracheostomy in multiple rib fracture patients.

Methods

A retrospective case-control study was used to analyze the clinical data of 222 patients with multiple rib fractures who underwent tracheotomy in the Affiliated Hospital of Yangzhou University from February 2015 to October 2021. According to the time from tracheal intubation to tracheostomy after admission, the patients were divided into two groups: the early tracheostomy group (within 7 days after tracheal intubation, ET) and late tracheostomy group (after the 7th day, LT). Propensity score matching (PSM) was used to eliminate the differences in baseline characteristics Logistic regression was used to predict the independent risk factors for early tracheostomy. Kaplan–Meier and Cox survival analyses were used to analyze the influencing factors of the 28-day survival.

Results

According to the propensity score matching analysis, a total of 174 patients were finally included in the study. Among them, there were 87 patients in the ET group and 87 patients in the LT group. After propensity score matching, Number of total rib fractures (NTRF) (P < 0.001), Acute respiratory distress syndrome (ARDS) (P < 0.001) and Volume of pulmonary contusion(VPC) (P < 0.000) in the ET group were higher than those in the LT group. Univariate analysis showed that the patients who underwent ET had a higher survival rate than those who underwent LT (P = 0.021). Pearson's analysis showed that there was a significant correlation between NTRF and VPC (r = 0.369, P = 0.001). A receiver operating characteristic(ROC)curve analysis showed that the areas under the curves were 0.832 and 0.804. The best cutoff-value values of the VPC and NTRF were 23.9 and 8.5, respectively. The Cox survival analysis showed that the timing of tracheostomy (HR = 2.51 95% CI, 1.12–5.57, P = 0.004) and age (HR = 1.53 95% CI, 1.00–2.05, P = 0.042) of the patients had a significant impact on the 28-day survival of patients with multiple rib fractures. In addition, The Kaplan–Meier survival analysis showed that the 28-day survival of patients in the ET group was significantly better than that of the LT group, P = 0.01.

Conclusions

NTRF, ADRS and VPC are independent risk factors for the timing and prognosis of early tracheotomy. A VPC ≥ 23.9% and/or an NTRF ≥ 8.5 could be used as predictors of ET in patients with multiple rib fractures. Predicting the timing of early tracheostomy also need prediction models in the future.
Why does patients’ discharge delay after vertebral augmentation? A factor analysis of 1,442 patients
Authors: Zhao He , Zhang Zhengping , Wang Yanjun , Qian Bing , Cao Xinhao , Yang Ming , Liu Yangjin , Zhao Qinpeng
Published: September 2022

Objective

Vertebral augmentation techniques are widely used to treat osteoporotic vertebral compression fractures (OVCFs). Superior analgesic effects and shortened bed rest time means patients recover quickly, but prolonged unscheduled hospitalization can increase medical expenses and the risk of bed rest complications. The aim of this study was to investigate the reasons for prolonged hospitalization after vertebral augmentation surgery and to determine the relative risk factors.

Methods

A single-center retrospective study was conducted to enroll patients with OVCFs and accepted vertebral augmentation surgery from January 2017 to December 2017. Clinical information was collected from the Hospital Information System (HIS). The criterion of delayed discharge was postoperative hospitalization more than 3 days. Telephone interviews and medical history evaluations were conducted to confirm the exact reason for retention. The risk factors were analyzed by multiple logistic regression.

Results

Overall, 1,442 patients were included, and 191 (13.2%) stayed in the hospital for more than 3 days postoperatively. The reasons for delayed discharge were psychological factors (37.2%), residual pain (32.5%), cardiopulmonary complications (15.7%), nonspecific symptoms (8.4%), incision abnormalities (2.6%), thrombosis (2.1%), and postanesthesia reactions (1.6%). The multiple logistic model was significant; age (OR 1.028; 95% CI 1.009–1.046), preoperative stay (OR 1.192; 95% CI 1.095–1.298), operation type (OR 1.494; 95% CI 1.019–2.189), and the number of surgical segments (OR 2.238; 95% CI 1.512–3.312) showed statistical significance. In contrast, gender (P > 0.1) and chronic comorbidities (P > 0.1) were not predictors in this model.

Conclusion

Overall, 13.2% of OVCF patients who underwent vertebral augmentation surgery were not discharged within 3 days postoperatively, and several predictors were found. Preoperative communication and comprehensive evaluations are calling for more attention; physicians should adopt an appropriate medical process to enhance rehabilitation in geriatric orthopedics.
Impact of preweaning vaccination on host gene expression and antibody titers in healthy beef calves
Authors: Scott Matthew A. , Woolums Amelia R. , Karisch Brandi B. , Harvey Kelsey M. , Capik Sarah F.
Published: September 2022

The impact of preweaning vaccination for bovine respiratory viruses on cattle health and subsequent bovine respiratory disease morbidity has been widely studied yet questions remain regarding the impact of these vaccines on host response and gene expression. Six randomly selected calves were vaccinated twice preweaning (T1 and T3) with a modified live vaccine for respiratory pathogens and 6 randomly selected calves were left unvaccinated. Whole blood samples were taken at first vaccination (T1), seven days later (T2), at revaccination and castration (T3), and at weaning (T4), and utilized for RNA isolation and sequencing. Serum from T3 and T4 was analyzed for antibodies to BRSV, BVDV1a, and BHV1. Sequenced RNA for all 48 samples was bioinformatically processed with a HISAT2/StringTie pipeline, utilizing reference guided assembly with the ARS-UCD1.2 bovine genome. Differentially expressed genes were identified through analyzing the impact of time across all calves, influence of vaccination across treatment groups at each timepoint, and the interaction of time and vaccination. Calves, regardless of vaccine administration, demonstrated an increase in gene expression over time related to specialized proresolving mediator production, lipid metabolism, and stimulation of immunoregulatory T-cells. Vaccination was associated with gene expression related to natural killer cell activity and helper T-cell differentiation, enriching for an upregulation in Th17-related gene expression, and downregulated genes involved in complement system activity and coagulation mechanisms. Type-1 interferon production was unaffected by the influence of vaccination nor time. To our knowledge, this is the first study to evaluate mechanisms of vaccination and development in healthy calves through RNA sequencing analysis.
Retention and future involvement in the American Kennel Club Junior Showmanship Program, a youth dog breed conformation competition
Authors: Loonsk Hannah L. , Clarke Dana L. , Battaglia Carmen , Otto Cynthia M.
Published: September 2022

Similar to other organizations that encourage positive youth development, the American Kennel Club (AKC) created the Junior Showmanship program to develop skills and pave a path for the next generation of competitors in canine conformation. Although participants age out of the Junior Showmanship Program when they turn eighteen, the hope is that Juniors will continue to be active in dog sports into adulthood. Females are overly represented in all age groups in this survey and in current participation. Although both males and females most commonly stopped participating at age 17 or 18, males had a significantly higher drop out at age 15. Further study is warranted to investigate factors specifically pertaining to male participation. A strong Belief System Model which accounted for positive responses associated with camaraderie and mentoring during the showmanship program was significantly (p=0.01) associated with commitment, member interest and active participation as an adult. In addition, success in the show ring was associated with future involvement in conformation or dog sports. Thus, both perceived achievement and mentorship by adult “volunteers” were associated with continued participation.
Greyhound morbidity and mortality in Australia: A descriptive analysis of reported data from regulatory racing agencies
Authors: Chang Vysie , Descovich Kris , Henning Joerg , Allavena Rachel
Published: September 2022

Commercial greyhound racing is legal in Australia but controversial due to concerns around animal welfare. To make evidence-based recommendations of animal welfare standards, a comprehensive analysis of available data on race events, animal health, injuries and fatalities is required. We undertook a review of publicly available data and reports published by official greyhound racing bodies for the purpose of determining how morbidity and mortality events associated with dog training and racing could be benchmarked. 6 years of available data from stewards' reports, quarterly and annual reports were descriptively analyzed from New South Wales, Victoria and Queensland. Whole-of-life tracking for individual dogs was sparse. Although stewards' reports were published in all three states, the availability of aggregated quarterly and annual reports varied. When available these provided additional information such as injury incidents standardized per thousand starts. In Queensland, quarterly and annual reports provided an overview of greyhound mortality and morbidity rates. In contrast with Victoria, quarterly reports were unavailable and only annual reports were published, meaning quarterly trends could not be determined. Therefore, injuries categorized by severity that were routinely included in quarterly reports in Queensland and New South Wales were unavailable in Victoria. Our findings demonstrate that data recording and reporting practices must be standardized to accurately evaluate whether animal welfare standards are being met in the Australian greyhound racing industry. Our recommendation is to have national standardized reporting of injuries and deaths, and a publicly available database for whole-of-life tracking for individual racing greyhounds.
Using Community Conversations to explore animal welfare perceptions and practices of rural households in Ethiopia
Authors: Lemma Mamusha , Doyle Rebecca , Alemayehu Gezahegn , Mekonnen Mesfin , Kumbe Adem , Wieland Barbara
Published: September 2022

There is a scarcity of data on animal welfare and its impact on livelihoods to inform animal welfare initiatives in Ethiopia. Perceptions and practices of rural households toward animal welfare are influenced by socio-cultural, demographic, and agroecological factors. We conducted Community Conversations in two geographically and culturally diverse regions of Ethiopia to explore the attitudes and practices of rural households regarding animal welfare and its impact on livelihoods. Community Conversations are facilitated dialogues among rural households to explore their perceptions, practices, constraints, and needs and identify and co-create solutions to improve the welfare of their animals. We used single- and mixed-sex discussion groups to understand community members' gendered perceptions of animal welfare and influence their attitudes and practices toward gender-equitable roles in animal welfare management. In the Community Conversations, community members readily described the biological needs of their animals but there was also a good acknowledgment of the behavioral and affective state needs of animals. Identified constraints for animal welfare included feed and water shortage, limited veterinary support, and poor animal handling practices. Community members described the welfare of their animals as being intertwined with their own livelihoods and identified productive, public health, and non-economic benefits of good animal welfare. Raising awareness of animal welfare within rural communities through Community Conversations is a useful way to both identify livestock production needs as well as engage community members in making practical improvements in animal welfare. The understanding of perceptions, practices, and needs of rural households in animal welfare helps engage communities in starting behavioral change and provides insights into developing context-specific welfare improvement interventions. Community Conversations are also an effective way to feedback community voices into planning to build a bottom-up implementation of animal welfare programs.
Entropy Generation of Secondary Flow in a Turning Passage with Different Boundary Layer Characteristics
Authors: Xi Yueyun , Peng Xu , Xia Hao , Sun Zhengzhong , Zhang Qiang
Published: September 2022

The development of secondary flow along a curved channel is a fundamental flow phenomenon occurring in a wide range of engineering applications, including turbomachinery, aerospace, heating, ventilation, air conditioning, etc. The underlying flow physics about end-wall secondary flows has been well-documented in the open literature, while the interaction between a secondary flow and a side-wall boundary layer, which is critical to the aerothermal performance of a side-wall surface, has not been comprehensively studied. In this study, the entropy generation of secondary flow and the interaction between an end-wall passage vortex and a side-wall boundary layer were numerically investigated by Reynolds-averaged Navier–Stokes (RANS) CFD for a 90° curved channel. The transportation effect of secondary flow and the generation mechanism of an induced vortex pair on the side wall is reported. It was also found that the growth of the secondary flow can be suppressed due to the displacement effect of the side-wall boundary layer. Furthermore, it was found that the interaction between a secondary flow and a side-wall boundary layer provides a suppression effect on side-wall boundary layer separation.
A Novel Decomposition Method for Manufacture Variations and the Sensitivity Analysis on Compressor Blades
Authors: Liu Baojie , Liu Jiaxin , Yu Xianjun , An Guangfeng
Published: September 2022

A high accuracy blade manufacture variation decomposition method was proposed to decompose the manufacture variations of compressor blades to systematic variation and non-systematic variation, which could help to clearly quantify the statistical characteristics of the effect of manufacture variations on the blade aerodynamic performance and to guide the modeling of manufacture variations in geometric uncertainty quantification and robust design studies. By conducting the decomposition of manufacture variations with 100 newly manufactured blades of a high-pressure compressor, it was found that the systematic variation could be modeled by using seven representative blade geometry design parameters well and the mean value of the non-systematic variation, which is determined by using the difference between the measured blade and systematically reconstructed blade, is close to zero. For the standard deviation of decomposed manufacture variations, the non-systematic variation accounts for about 40% of the whole, indicating that the systematic variation is the major component of the manufacture variation. However, based on statistical analysis and sensitivity analysis of the effects of the two types of manufacture variations on blade aerodynamic performance, it was found that the mean deviation of the blade loss mainly derives from systematic variations, and the loss dispersion caused by non-systematic variations is significantly greater than that caused by systematic variations. Furthermore, the blade loss at the high incidence angle is most sensitive to the inlet metal angle which belongs to the systematic variation. Meanwhile, the non-systematic variation near the leading-edge is the most sensitive, and it contributes to most of the performance disperse but only accounts for a geometric variation of about 0.45%.
EEG Feature Analysis Related to Situation Awareness Assessment and Discrimination
Authors: Feng Chuanyan , Liu Shuang , Wanyan Xiaoru , Chen Hao , Min Yuchen , Ma Yilan
Published: September 2022

In order to discriminate situation awareness (SA) levels on the basis of SA-sensitive electroencephalography (EEG) features, the high-SA (HSA) group and low-SA (LSA) groups, which are representative of two SA levels, were classified according to the situation awareness global assessment technology (SAGAT) scores measured in the multi-attribute task battery (MATB) II tasks. Furthermore, three types of EEG features, namely, absolute power, relative power, and slow-wave/fast-wave (SW/FW), were explored using spectral analysis. In addition, repeated analysis of variance (ANOVA) was conducted in three brain regions (frontal, central, and parietal) × three brain lateralities (left, middle, and right) × two SA groups (LSA and HSA) to explore SA-sensitive EEG features. The statistical results indicate a significant difference between the two SA groups according to SAGAT scores; moreover, no significant difference was found for the absolute power of four waves (delta (δ), theta (θ), alpha (α), and beta (β)). In addition, the LSA group had a significantly lower β relative power than the HSA group in central and partial regions. Lastly, compared with the HSA group, the LSA group had higher θ/β and (θ + α)/(α + β) in all analyzed brain regions, higher α/β in the parietal region, and higher (θ + α)/β in all analyzed regions except for the left and right laterality in the frontal region. The above SA-sensitive EEG features were fed into principal component analysis (PCA) and the Bayes method to discriminate different SA groups, and the accuracies were 83.3% for the original validation and 70.8% for the cross-validation. The results provide a basis for real-time assessment and discrimination of SA by investigating EEG features, thus contributing to monitoring SA decrement that might lead to threats to flight safety.
Numerical Investigation on Mechanisms of MHD Heat Flux Mitigation in Hypersonic Flows
Authors: Zhou Zhifeng , Zhang Zhichao , Gao Zhenxun , Xu Ke , Lee Chun-Hian
Published: September 2022

Numerical simulations of hypersonic magnetohydrodynamics (MHD) flow over a typical sphere–cone blunt body are carried out based on the assumption of a low magnetic Reynolds number. The effects of an external dipole magnetic field on the surface heat flux are analyzed in detail, and multiple mechanisms of the MHD heat flux mitigation are revealed systematically for the first time. The following is found: (1) The external magnetic field can effectively reduce the stagnation point heat flux, and the increase in the boundary layer thickness due to the effect of counter-flow Lorentz force, which is equivalent to adding an adverse pressure gradient, is the main reason. (2) In the head region of the blunt body, the relative surface heat flux shows a complex trend of rising and falling because there are two mechanisms which could produce the opposite effects on the surface heat flux. One is that the counter-flow Lorentz force results in an increase in the boundary layer thickness, and the other is that the Joule heating increases the static temperature behind the shock wave. (3) In the shoulder region of the blunt body, the Lorentz force component, normal to streamline, could change the flow direction of the fluid elements, causing the streamline to deviate from the wall or even separate, thus affecting the surface heat flux. (4) In the large area downstream of the blunt body, the surface heat flux could still be reduced by more than 30% due to the “upstream historical effect”.
Evolution of Genetics Organisations’ Strategies through the Implementation of Genomic Selection: Learnings and Prospects
Authors: Banks Robert
Published: September 2022

Since its initial description in 2001, and with falling costs of genotyping, genomic selection has been implemented in a wide range of species. Theory predicts that the genomic selection approach to genetic improvement offers scope both for faster progress and the opportunity to make change in traits formerly less tractable to selection (hard-to-measure traits). This paper reports a survey of organisations involved in genetic improvement, across species, countries, and roles both public and private. While there are differences across organisations in what have been the most significant outcomes to date, both the increased accuracy of breeding values that underpins potentially faster progress, and the re-balancing of genetic change to include real progress in the hard-to-measure traits, have been widely observed. Across organisations, learnings have included the increasing importance of investment in phenotyping, and opportunities to evolve business models to engage more directly with a wider range of stakeholders. Genomic selection can be considered a more modular approach to genetic improvement, and its simplicity and effectiveness can transform both genetic improvement and the effectiveness of multi-disciplinary approaches to improving livestock and plant production, enabling potentially very significant increases in agricultural productivity, profitability and sustainability.
Quantitative Electroencephalogram (qEEG) as a Natural and Non-Invasive Window into Living Brain and Mind in the Functional Continuum of Healthy and Pathological Conditions
Authors: Fingelkurts Alexander A. , Fingelkurts Andrew A.
Published: September 2022

Many practicing clinicians are time-poor and are unaware of the accumulated neuroscience developments. Additionally, given the conservative nature of their field, key insights and findings trickle through into the mainstream clinical zeitgeist rather slowly. Over many decades, clinical, systemic, and cognitive neuroscience have produced a large and diverse body of evidence for the potential utility of brain activity (measured by electroencephalogram—EEG) for neurology and psychiatry. Unfortunately, these data are enormous and essential information often gets buried, leaving many researchers stuck with outdated paradigms. Additionally, the lack of a conceptual and unifying theoretical framework, which can bind diverse facts and relate them in a meaningful way, makes the whole situation even more complex. To contribute to the systematization of essential data (from the authors’ point of view), we present an overview of important findings in the fields of electrophysiology and clinical, systemic, and cognitive neuroscience and provide a general theoretical–conceptual framework that is important for any application of EEG signal analysis in neuropsychopathology. In this context, we intentionally omit detailed descriptions of EEG characteristics associated with neuropsychopathology as irrelevant to this theoretical–conceptual review.
Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea
Authors: Li Hua-Xiang , Wang Juan-Juan , Lu Chun-Lei , Gao Ya-Jun , Gao Lu , Yang Zhen-Quan
Published: September 2022

Antrodia cinnamomea is a precious and popular edible and medicinal mushroom. It has attracted increasing attention due to its various and excellent bioactivities, such as hepatoprotection, hypoglycemic, antioxidant, antitumor, anticancer, anti-inflammatory, immunomodulation, and gut microbiota regulation properties. To elucidate its bioactivities and develop novel functional foods or medicines, numerous studies have focused on the isolation and identification of the bioactive compounds of A. cinnamomea. In this review, the recent advances in bioactivity, isolation, purification, and identification methods of active compounds from A. cinnamomea were summarized. The present work is beneficial to the further isolation and discovery of new active compounds from A. cinnamomea.
Real-Time Temperature Rise Estimation during Irreversible Electroporation Treatment through State-Space Modeling
Authors: Campelo Sabrina N. , Jacobs Edward J. , Aycock Kenneth N. , Davalos Rafael V.
Published: September 2022

To evaluate the feasibility of real-time temperature monitoring during an electroporation-based therapy procedure, a data-driven state-space model was developed. Agar phantoms mimicking low conductivity (LC) and high conductivity (HC) tissues were tested under the influences of high (HV) and low (LV) applied voltages. Real-time changes in impedance, measured by Fourier Analysis SpecTroscopy (FAST) along with the known tissue conductivity and applied voltages, were used to train the model. A theoretical finite element model was used for external validation of the model, producing model fits of 95.8, 88.4, 90.7, and 93.7% at 4 mm and 93.2, 58.9, 90.0, and 90.1% at 10 mm for the HV-HC, LV-LC, HV-LC, and LV-HC groups, respectively. The proposed model suggests that real-time temperature monitoring may be achieved with good accuracy through the use of real-time impedance monitoring.
NICEFIT—A Prospective, Non-Interventional, and Multicentric Study for the Management of Idiopathic Pulmonary Fibrosis with Antifibrotic Therapy in Taiwan
Authors: Cheng Shih-Lung , Sheu Chau-Chyun , Chian Chih-Feng , Hsu Jeng-Yuan , Kao Kuo-Chin , Hang Liang-Wen , Lin Ching-Hsiung , Fang Wen-Feng , Wang Hao-Chien , Perng Diahn-Warng
Published: September 2022

Idiopathic pulmonary fibrosis (IPF) causes progressive lung fibrosis with subsequent fatality and has limited treatment options. NICEFIT is the first Taiwan-based prospective, observational, and non-interventional registry for IPF progression under routine clinical practice in Taiwan. Data on 101 patients (aged 74.6 ± 9.1 years and 83.2% men) with IPF were collected over 2 years (2018−2020) from medical centers in Taiwan at baseline, 1 month, and subsequent 3-month intervals. Treated patients (n = 88) received the antifibrotics nintedanib or pirfenidone, compared with the untreated group (n = 13). The 2-year assessment revealed overall preserved lung functionality in the treated patients, with insignificant changes from baseline for percent predicted forced vital capacity or FVC (±1.7%). The presence of respiratory comorbidities significantly increased the risk of both AE and death (with or without AE) over the full study duration. Furthermore, the decline of predicted FVC significantly increased with the risk of acute exacerbations (AE) in the second year. Overall, antifibrotic medication was beneficial in stalling IPF progression, reducing AEs, and delaying mortality in the treated cohort, despite their lower baseline lung functions. Further, no new safety concerns over antifibrotic treatments were observed for the Taiwanese population.
The Impact of Exposure Profile on the Efficacy of Dual Amylin and Calcitonin Receptor Agonist Therapy
Authors: Sonne Nina , Larsen Anna Thorsø , Karsdal Morten Asser , Henriksen Kim
Published: September 2022

Background: Dual Amylin and Calcitonin Receptor Agonists (DACRAs) are treatment candidates for obesity and type 2 diabetes. Recently, a once-weekly DACRA (KBP-A) showed promise, potentially due to its different exposure profile compared to daily DACRA (KBP). Parathyroid hormone, a G-protein-coupled receptor (GPCR) class B agonist, is an example of the exposure profile being critical to the effect. Since KBP and KBP-A also activate GPCR class B, we compared the effects of injection to continuous infusion of short-acting KBP and long-acting KBP-A in obese and diabetic rats to shed light on the role of exposure profiles. Methods: To explore the metabolic benefits of dose optimization, the following dosing profiles were compared in High Fat Diet (HFD)-fed Sprague–Dawley rats and diabetic Zucker Diabetic Fatty (ZDF) rats: (1) KBP dosed once-daily by injection or by continuous infusion in HFD and ZDF rats; (2) KBP injected once-daily and KBP-A injected once every 3rd day (Q3D) in HFD rats; (3) KBP-A injected Q3D or by infusion in ZDF rats. Results: KBP and KBP-A, delivered by either injection or infusion, resulted in similar weight and food intake reductions in HFD rats. In ZDF rats, injection of KBP improved glucose control significantly compared to infusion, while delivery of KBP-A by injection and continuous infusion was comparable in terms of glucose control. Conclusion: different dosing profiles of KBP and KBP-A had no impact on metabolic benefits in HFD rats. In diabetic ZDF rats, KBP by injection instead of infusion was superior, while for KBP-A the effects were similar.
IL-18 Gene rs187238 and rs1946518 Polymorphisms and Expression in Gingival Tissue in Patients with Periodontitis
Authors: Mazurek-Mochol Małgorzata , Brzeska Magdalena , Serwin Karol , Malinowski Damian , Safranow Krzysztof , Zagrodnik Edyta , Stecewicz Iwona , Pawlik Andrzej
Published: September 2022

Periodontitis is a chronic disease with disturbed balance between the immune and inflammatory response of the host to bacteria. Many studies have shown that proinflammatory cytokines play a significant role in the pathogenesis of periodontal disease. In this study, we examined the association between the IL-18 gene rs187238 and rs1946518 polymorphisms and periodontitis in non-smoking and smoking patients. This study enrolled 200 patients with periodontitis (130 non-smokers and 70 smokers) and 156 control subjects (124 non-smokers and 32 smokers). There were no statistically significant differences in the distribution of the rs187238 and rs1946518 IL-18 genotypes and alleles between patients with periodontitis and control subjects, between smoking patients with periodontitis and smoking control subjects, and between non-smoking patients with periodontitis and non-smoking control subjects. There were no statistically significant differences in clinical parameters in relation to the IL18 rs187238 genotypes. In patients with the IL18 rs1946518 GG genotype, we observed increased values of bleeding on probing (BoP) and periodontal probing depth (PPD), compared to subjects with the TT genotype. In patients with periodontitis, we observed statistically significant decreased expression of the IL-18 gene in comparison with healthy subjects (0.231 ± 0.163 vs. 0.663 ± 0.197, p = 0.0008). In addition, the IL-18 gene expression in gingival tissue in patients with periodontitis correlated positively with the number of remaining teeth. The results of our study suggest that the IL-18 rs187238 and rs1946518 polymorphisms are not significant risk indicators of periodontitis in our population. However, in patients with the IL18 rs1946518 GG genotype, we observed increased values of BoP and PPD, compared to subjects with the TT genotype. In addition, in gingival tissue of patients with periodontitis, we have detected decreased expression of IL-18. The gingival expression of IL-18 in patients with periodontitis correlated positively with number of remaining teeth. The above results suggest that IL-18, in addition to its pro-inflammatory effects in periodontal disease, may also exhibit protective properties.
Chronic Kidney Disease—An Underestimated Risk Factor for Antimicrobial Resistance in Patients with Urinary Tract Infections
Authors: Vacaroiu Ileana Adela , Cuiban Elena , Geavlete Bogdan Florin , Gheorghita Valeriu , David Cristiana , Ene Cosmin Victor , Bulai Catalin , Lupusoru Gabriela Elena , Lupusoru Mircea , Balcangiu-Stroescu Andra Elena , Feier Larisa Florina , Simion Ioana Sorina , Radulescu Daniela
Published: September 2022

(1) Background: Chronic kidney disease (CKD), as well as antimicrobial resistance (AMR) represent major global health problems, with important social and economic implications. It was reported that CKD is a risk factor for antimicrobial resistance, but evidence is scarce. In addition, CKD is recognized to be a risk factor for complicated urinary tract infections (UTIs). (2) Methods: We conducted an observational study on 564 adult in-hospital patients diagnosed with urinary tract infections. The aim of the study was to identify the risk factors for AMR, as well as multiple drug resistance (MDR) and the implicated resistance patterns. (3) Results: The mean age was 68.63 ± 17.2 years. The most frequently isolated uropathogens were Escherichia coli strains (68.3%) followed by Klebsiella species (spp. (11.2%). In 307 cases (54.4%)), the UTIs were determined by antibiotic-resistant bacteria (ARBs) and 169 cases (30%) were UTIs with MDR strains. Increased age (≥65) OR 2.156 (95% CI: 1.404–3.311), upper urinary tract obstruction OR 1.666 (1.083–2.564), indwelling urinary catheters OR 6.066 (3.919–9.390), chronic kidney disease OR 2.696 (1.832–3.969), chronic hemodialysis OR 4.955 (1.828–13.435) and active malignancies OR 1.962 (1.087–3.540) were independent risk factors for MDR UTIs. In a multivariate logistic regression model, only indwelling urinary catheters (OR 5.388, 95% CI: 3.294–8.814, p < 0.001), CKD (OR 1.779, 95% CI: 1.153–2.745, p = 0.009) and chronic hemodialysis (OR 4.068, 95% 1.413–11.715, p = 0.009) were risk factors for UTIs caused by MDR uropathogens. (4) Conclusions: CKD is an important risk factor for overall antimicrobial resistance, but also for multiple-drug resistance.
Serum CCL2 Is a Prognostic Biomarker for Non-Metastatic Castration-Sensitive Prostate Cancer
Authors: Iwamoto Hiroaki , Izumi Kouji , Nakagawa Ryunosuke , Toriumi Ren , Aoyama Shuhei , Kamijima Taiki , Shimada Takafumi , Kano Hiroshi , Makino Tomoyuki , Naito Renato , Kadomoto Suguru , Yaegashi Hiroshi , Kawaguchi Shohei , Nohara Takahiro , Shigehara Kazuyoshi , Kadono Yoshifumi , Mizokami Atsushi
Published: September 2022

Purpose: Prostate-specific antigen (PSA) is a useful prostate cancer (PC) biomarker, but some cases reported that PSA does not correlate with the Gleason score. Serum chemokine (CC motif) ligand 2 (CCL2) has been reported to be a potential complementary PSA biomarker, but it remains unclear whether it can be applied to non-metastatic castration-sensitive prostate cancer (nmCSPC) or each section of the stages. Serum CCL2′s usefulness was investigated as a prognostic nmCSPC biomarker in this study. Methods: Serum samples were collected from 379 patients who underwent prostate biopsy at Kanazawa University Hospital from 2007 to 2013. A total of 230 patients with nmCSPC were included in this study of the 255 patients with histologically diagnosed prostate cancer. The serum CCL2 efficacy as a prognostic nmCSPC biomarker was investigated retrospectively. Results: An independent significant predictor of worse OS was CCL2 ≥ 280 pg/dL and CRP ≥ 0.5 mg/dL in multivariate analysis. Gleason score ≥ 8 and CCL2 ≥ 280 pg/dL were independent significant predictors of CRPC-free survival (CFS) worsening in multivariate analysis. Serum CCL2 was a predictive biomarker for OS and CFS in nmCSPC. Furthermore, CCL2 ≥ 280 pg/mL patients had significantly worse visceral metastasis-free survival than those with CCL2 < 280 pg/mL. Conclusion: This study is the first to demonstrate serum CCL2 utility as a biomarker to predict OS and CFS in nmCSPC.
Modulation of Hedgehog Signaling for the Treatment of Basal Cell Carcinoma and the Development of Preclinical Models
Authors: Dukes Meghan W. , Meade Thomas J.
Published: September 2022

Basal Cell Carcinoma (BCC) is the most commonly diagnosed cancer worldwide. While the survivability of BCC is high, many patients are excluded from clinically available treatments due to health risks or personal choice. Further, patients with advanced or metastatic disease have severely limited treatment options. The dysregulation of the Hedgehog (Hh) signaling cascade drives onset and progression of BCC. As such, the modulation of this pathway has driven advancements in BCC research. In this review, we focus firstly on inhibitors that target the Hh pathway as chemotherapeutics against BCC. Two therapies targeting Hh signaling have been made clinically available for BCC patients, but these treatments suffer from limited initial efficacy and a high rate of chemoresistant tumor recurrence. Herein, we describe more recent developments of chemical scaffolds that have been designed to hopefully improve upon the available therapeutics. We secondly discuss the history and recent efforts involving modulation of the Hh genome as a method of producing in vivo models of BCC for preclinical research. While there are many advancements left to be made towards improving patient outcomes with BCC, it is clear that targeting the Hh pathway will remain at the forefront of research efforts in designing more effective chemotherapeutics as well as relevant preclinical models.
Tissue Rigidity Increased during Carcinogenesis of NTCU-Induced Lung Squamous Cell Carcinoma In Vivo
Authors: Zakaria Muhammad Asyaari , Aziz Jazli , Rajab Nor Fadilah , Chua Eng Wee , Masre Siti Fathiah
Published: September 2022

Increased tissue rigidity is an emerging hallmark of cancer as it plays a critical role in promoting cancer growth. However, the field lacks a defined characterization of tissue rigidity in dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) in vivo. Pre-malignant and malignant lung SCC was developed in BALB/c mice using N-nitroso-tris-chloroethylurea (NTCU). Picro sirius red staining and atomic force microscopy were performed to measure collagen content and collagen (diameter and rigidity), respectively. Then, the expression of tenascin C (TNC) protein was determined using immunohistochemistry staining. Briefly, all tissue rigidity parameters were found to be increased in the Cancer group as compared with the Vehicle group. Importantly, collagen content (33.63 ± 2.39%) and TNC expression (7.97 ± 2.04%) were found to be significantly higher (p < 0.05) in the Malignant Cancer group, as compared with the collagen content (18.08 ± 1.75%) and TNC expression (0.45 ± 0.53%) in the Pre-malignant Cancer group, indicating increased tissue rigidity during carcinogenesis of lung SCC. Overall, tissue rigidity of lung SCC was suggested to be increased during carcinogenesis as indicated by the overexpression of collagen and TNC protein, which may warrant further research as novel therapeutic targets to treat lung SCC effectively.
Rational Design of Nanozymes Enables Advanced Biochemical Sensing
Authors: Liu Jinjin , Niu Xiangheng
Published: September 2022

In comparison with bioenzymes, nanozymes exhibit excellent robustness against extreme conditions, a low production cost, and easy-to-adjust properties, as well as potential versatility. These superiorities have attracted abundant interest in the last 15 years, to develop various nanozymes for applications including analytical sensing, environmental engineering, and biomedicine. In particular, for analytical sensing, a lot of nanozyme-involved principles and methods have been explored and applied to clinical diagnosis, environmental monitoring, food safety detection, and forensic analysis. Moreover, rational exploitation and use of nanozyme materials promote the performance of analytical methods. To highlight the latest progress in this attractive field, recent design concepts of nanozymes for advanced biochemical sensing are summarized. The development of single-atom nanozymes, self-cascade nanozymes, structurally biomimetic nanozymes, molecularly imprinted nanozymes, nanozymes breaking the pH limit, and multifunctional nanozymes is discussed in detail, to enhance detection sensitivity and selectivity, as well as expand application scenarios. Finally, some challenges and trends related to nanozyme-based sensors are reported, to satisfy the increasing needs of biochemical analysis with nanozymes.