bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9
Authors: Yang Yuting , Zhang Yun , Yang Changcheng , Fang Fang , Wang Ying , Chang Haiyan , Chen Ze , Chen Ping
Published: February 2021

Background

Both the highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic avian influenza (LPAI) H9N2 viruses have been reported to cross species barriers to infect humans. H5N1 viruses can cause severe damage and are associated with a high mortality rate, but H9N2 viruses do not cause such outcomes. Our purpose was to use proteomics technology to study the differential expression of mitochondrial-related proteins related to H5N1 and H9N2 virus infections.

Methods

According to the determined viral infection titer, A549 cells were infected with 1 multiplicity of infection virus, and the mitochondria were extracted after 24 h of incubation. The protein from lysed mitochondria was analyzed by the BCA method to determine the protein concentration, as well as SDS-PAGE (preliminary analysis), two-dimensional gel electrophoresis, and mass spectrometry. Differential protein spots were selected, and Western blotting was performed to verify the proteomics results. The identified proteins were subjected to GO analysis for subcellular localization, KEGG analysis for functional classification and signaling pathways assessment, and STRING analysis for functional protein association network construction.

Results

In the 2-D gel electrophoresis analysis, 227 protein spots were detected in the H5N1-infected group, and 169 protein spots were detected in the H9N2-infected group. Protein spots were further subjected to mass spectrometry identification and removal of redundancy, and 32 differentially expressed proteins were identified. Compared with the H9N2 group, the H5N1-infected group had 16 upregulated mitochondrial proteins and 16 downregulated proteins. The differential expression of 70-kDa heat shock protein analogs, short-chain enoyl-CoA hydratase, malate dehydrogenase, and ATP synthase was verified by Western blot, and the results were consistent with the proteomics findings. Functional analysis indicated that these differentially expressed proteins were primarily involved in apoptosis and metabolism.

Conclusions

Compared with their expression in the H9N2 group, the differential expression of eight mitochondrial proteins in the H5N1 group led to host T cell activation, antigen presentation, stress response, ATP synthesis and cell apoptosis reduction, leading to higher pathogenicity of H5N1 than H9N2.
Neurofibromin expression by normal salivary glands
Authors: Luna Eloá Borges , Montovani Pâmella Pinho , Rozza-de-Menezes Rafaela Elvira , Cunha Karin Soares
Published: February 2021

Introduction

Neurofibromin, a protein encoded by theNF1gene, is mutated in neurofibromatosis 1, one of the most common genetic diseases. Oral manifestations are common and a high prevalence of hyposalivation was recently described in individuals with neurofibromatosis 1. Although neurofibromin is ubiquitously expressed, its expression levels vary depending on the tissue type and developmental stage of the organism. The role of neurofibromin in the development, morphology, and physiology of salivary glands is unknown and a detailed expression of neurofibromin in human normal salivary glands has never been investigated.

Aim

To investigate the expression levels and distribution of neurofibromin in acinar and ductal cells of major and minor salivary glands of adult individuals without NF1.

Material and method

Ten samples of morphologically normal major and minor salivary glands (three samples of each gland: parotid, submandibular and minor salivary; and one sample of sublingual gland) from individuals without neurofibromatosis 1 were selected to assess neurofibromin expression through immunohistochemistry. Immunoquantification was performed by a digital method.

Results

Neurofibromin was expressed in the cytoplasm of both serous and mucous acinar cells, as well as in ducts from all the samples of salivary glands. Staining intensity varied from mild to strong depending on the type of salivary gland and region (acini or ducts). Ducts had higher neurofibromin expression than acinar cells (p = 0.003). There was no statistical association between the expression of neurofibromin and the type of the salivary gland, considering acini (p = 0.09) or ducts (p = 0.50) of the four salivary glands (parotid, submandibular, minor salivary, and sublingual gland). Similar results were obtained comparing the acini (p = 0.35) and ducts (p = 0.50) of minor and major salivary glands. Besides, there was no correlation between the expression of neurofibromin and age (p = 0.08), and sex (p = 0.79) of the individuals, considering simultaneously the neurofibromin levels of acini and duct (n = 34).

Conclusion

Neurofibromin is expressed in the cytoplasm of serous and mucous acinar cells, and ductal cells of salivary glands, suggesting that this protein is important for salivary gland function.
Correction to: Mechanical ventilation of patients in helicopter emergency medical service transport: an international survey
Authors: Hilbert-Carius Peter , Struck Manuel F. , Hofer Veronika , Hinkelbein Jochen , Rognås Leif , Adler Jörn , Christian Michael D. , Wurmb Thomas , Bernhard Michael , Hossfeld Björn
Published: February 2021

An amendment to this paper has been published and can be accessed via the original article.
Multi-modal imaging of high-risk ductal carcinoma in situ of the breast using C2Am: a targeted cell death imaging agent
Authors: Szucs Zoltan , Joseph James , Larkin Tim J. , Xie Bangwen , Bohndiek Sarah E. , Brindle Kevin M. , Neves André A.
Published: February 2021

Background

Ductal carcinoma in situ (DCIS) is a non-invasive form of early breast cancer, with a poorly understood natural history of invasive transformation. Necrosis is a well-recognized adverse prognostic feature of DCIS, and non-invasive detection of its presence and spatial extent could provide information not obtainable by biopsy. We describe here imaging of the distribution and extent of comedo-type necrosis in a model of human DCIS using C2Am, an imaging agent that binds to the phosphatidylserine exposed by necrotic cells.

Methods

We used an established xenograft model of human DCIS that mimics the histopathological features of the disease. Planar near-infrared and optoacoustic imaging, using fluorescently labeled C2Am, were used to image non-invasively the presence and extent of lesion necrosis.

Results

C2Am showed specific and sensitive binding to necrotic areas in DCIS tissue, detectable both in vivo and ex vivo. The imaging signal generated in vivo using near-infrared (NIR) fluorescence imaging was up to 6-fold higher in DCIS lesions than in surrounding fat pad or skin tissue. There was a correlation between the C2Am NIR fluorescence (Pearson R = 0.783, P = 0.0125) and optoacoustic signals (R > 0.875, P < 0.022) in the DCIS lesions in vivo and the corresponding levels of cell death detected histologically.

Conclusions

C2Am is a targeted multi-modal imaging agent that could complement current anatomical imaging methods for detecting DCIS. Imaging the presence and spatial extent of necrosis may give better prognostic information than that obtained by biopsy alone.
The colorectal cancer-associated faecal microbiome of developing countries resembles that of developed countries
Authors: Young Caroline , Wood Henry M. , Seshadri Ramakrishnan Ayloor , Van Nang Pham , Vaccaro Carlos , Melendez Luis Contreras , Bose Mayilvahanan , Van Doi Mai , Piñero Tamara Alejandra , Valladares Camilo Tapia , Arguero Julieta , Balaguer Alba Fuentes , Thompson Kelsey N. , Yan Yan , Huttenhower Curtis , Quirke Philip
Published: February 2021

Background

The incidence of colorectal cancer (CRC) is increasing in developing countries, yet limited research on the CRC- associated microbiota has been conducted in these areas, in part due to scarce resources, facilities, and the difficulty of fresh or frozen stool storage/transport. Here, we aimed (1) to establish a broad representation of diverse developing countries (Argentina, Chile, India, and Vietnam); (2) to validate a ‘resource-light’ sample-collection protocol translatable in these settings using guaiac faecal occult blood test (gFOBT) cards stored and, importantly, shipped internationally at room temperature; (3) to perform initial profiling of the collective CRC-associated microbiome of these developing countries; and (4) to compare this quantitatively with established CRC biomarkers from developed countries.

Methods

We assessed the effect of international storage and transport at room temperature by replicating gFOBT from five UK volunteers, storing two in the UK, and sending replicates to institutes in the four countries. Next, to determine the effect of prolonged UK storage, DNA extraction replicates for a subset of samples were performed up to 252 days apart. To profile the CRC-associated microbiome of developing countries, gFOBT were collected from 41 treatment-naïve CRC patients and 40 non-CRC controls from across the four institutes, and V4 16S rRNA gene sequencing was performed. Finally, we constructed a random forest (RF) model that was trained and tested against existing datasets from developed countries.

Results

The microbiome was stably assayed when samples were stored/transported at room temperature and after prolonged UK storage. Large-scale microbiome structure was separated by country and continent, with a smaller effect from CRC. Importantly, the RF model performed similarly to models trained using external datasets and identified similar taxa of importance (Parvimonas, Peptostreptococcus, Fusobacterium, Alistipes, and Escherichia).

Conclusions

This study demonstrates that gFOBT, stored and transported at room temperature, represents a suitable method of faecal sample collection for amplicon-based microbiome biomarkers in developing countries and suggests a CRC-faecal microbiome association that is consistent between developed and developing countries.
Novel DNA methylation signatures of tobacco smoking with trans-ethnic effects
Authors: Christiansen C. , Castillo-Fernandez J. E. , Domingo-Relloso A. , Zhao W. , El-Sayed Moustafa J. S. , Tsai P.-C. , Maddock J. , Haack K. , Cole S. A. , Kardia S. L. R. , Molokhia M. , Suderman M. , Power C. , Relton C. , Wong A. , Kuh D. , Goodman A. , Small K. S. , Smith J. A. , Tellez-Plaza M. , Navas-Acien A. , Ploubidis G. B. , Hardy R. , Bell J. T.
Published: February 2021

Background

Smoking remains one of the leading preventable causes of death. Smoking leaves a strong signature on the blood methylome as shown in multiple studies using the Infinium HumanMethylation450 BeadChip. Here, we explore novel blood methylation smoking signals on the Illumina MethylationEPIC BeadChip (EPIC) array, which also targets novel CpG-sites in enhancers.

Method

A smoking-methylation meta-analysis was carried out using EPIC DNA methylation profiles in 1407 blood samples from four UK population-based cohorts, including the MRC National Survey for Health and Development (NSHD) or 1946 British birth cohort, the National Child Development Study (NCDS) or 1958 birth cohort, the 1970 British Cohort Study (BCS70), and the TwinsUK cohort (TwinsUK). The overall discovery sample included 269 current, 497 former, and 643 never smokers. Replication was pursued in 3425 trans-ethnic samples, including 2325 American Indian individuals participating in the Strong Heart Study (SHS) in 1989–1991 and 1100 African-American participants in the Genetic Epidemiology Network of Arteriopathy Study (GENOA).

Results

Altogether 952 CpG-sites in 500 genes were differentially methylated between smokers and never smokers after Bonferroni correction. There were 526 novel smoking-associated CpG-sites only profiled by the EPIC array, of which 486 (92%) replicated in a meta-analysis of the American Indian and African-American samples. Novel CpG sites mapped both to genes containing previously identified smoking-methylation signals and to 80 novel genes not previously linked to smoking, with the strongest novel signal in SLAMF7. Comparison of former versus never smokers identified that 37 of these sites were persistently differentially methylated after cessation, where 16 represented novel signals only profiled by the EPIC array. We observed a depletion of smoking-associated signals in CpG islands and an enrichment in enhancer regions, consistent with previous results.

Conclusion

This study identified novel smoking-associated signals as possible biomarkers of exposure to smoking and may help improve our understanding of smoking-related disease risk.
BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer
Authors: Giles Katherine A. , Gould Cathryn M. , Achinger-Kawecka Joanna , Page Scott G. , Kafer Georgia R. , Rogers Samuel , Luu Phuc-Loi , Cesare Anthony J. , Clark Susan J. , Taberlay Phillippa C.
Published: February 2021

Background

BRG1 (encoded by SMARCA4) is a catalytic component of the SWI/SNF chromatin remodelling complex, with key roles in modulating DNA accessibility. Dysregulation of BRG1 is observed, but functionally uncharacterised, in a wide range of malignancies. We have probed the functions of BRG1 on a background of prostate cancer to investigate how BRG1 controls gene expression programmes and cancer cell behaviour.

Results

Our investigation of SMARCA4 revealed that BRG1 is over-expressed in the majority of the 486 tumours from The Cancer Genome Atlas prostate cohort, as well as in a complementary panel of 21 prostate cell lines. Next, we utilised a temporal model of BRG1 depletion to investigate the molecular effects on global transcription programmes. Depleting BRG1 had no impact on alternative splicing and conferred only modest effect on global expression. However, of the transcriptional changes that occurred, most manifested as down-regulated expression. Deeper examination found the common thread linking down-regulated genes was involvement in proliferation, including several known to increase prostate cancer proliferation (KLK2, PCAT1 and VAV3). Interestingly, the promoters of genes driving proliferation were bound by BRG1 as well as the transcription factors, AR and FOXA1. We also noted that BRG1 depletion repressed genes involved in cell cycle progression and DNA replication, but intriguingly, these pathways operated independently of AR and FOXA1. In agreement with transcriptional changes, depleting BRG1 conferred G1 arrest.

Conclusions

Our data have revealed that BRG1 promotes cell cycle progression and DNA replication, consistent with the increased cell proliferation associated with oncogenesis.
DNA methylation profiles of bronchoscopic biopsies for the diagnosis of lung cancer
Authors: Goldmann Torsten , Schmitt Bernhard , Müller Julia , Kröger Maren , Scheufele Swetlana , Marwitz Sebastian , Nitschkowski Dörte , Schneider Marc A. , Meister Michael , Muley Thomas , Thomas Michael , Kugler Christian , Rabe Klaus F. , Siebert Reiner , Reck Martin , Ammerpohl Ole
Published: February 2021

Background

Lung cancer is the leading cause of cancer-related death in most western countries in both, males and females, accounting for roughly 20–25% of all cancer deaths. For choosing the most appropriate therapy regimen a definite diagnosis is a prerequisite. However, histological characterization of bronchoscopic biopsies particularly with low tumor cell content is often challenging. Therefore, this study aims at (a) determining the value of DNA methylation analysis applied to specimens obtained by bronchoscopic biopsy for the diagnosis of lung cancer and (b) at comparing aberrantly CpG loci identified in bronchoscopic biopsy with those identified by analyzing surgical specimens.

Results

We report the HumanMethylation450-based DNA methylation analysis of paired samples of bronchoscopic biopsy specimens either from the tumor side or from the contralateral tumor-free bronchus in 37 patients with definite lung cancer diagnosis and 18 patients with suspicious diagnosis. A differential DNA methylation analysis between both biopsy sites of patients with definite diagnosis identified 1303 loci. Even those samples were separated by the set of 1303 loci in which histopathological analysis could not unambiguously define the dignity. Further differential DNA methylation analyses distinguished between SCLC and NSCLC. We validated our results in an independent cohort of 40 primary lung cancers obtained by open surgical resection and their corresponding controls from the same patient as well as in publically available DNA methylation data from a TCGA cohort which could also be classified with high accuracy.

Conclusions

Considering that the prognosis correlates with tumor stage at time of diagnosis, early detection of lung cancer is vital and DNA methylation analysis might add valuable information to reliably characterize lung cancer even in histologically ambiguous sample material.
Effects of microbial organic fertilizers on Astragalus membranaceus growth and rhizosphere microbial community
Authors: Liang Jian-Ping , Xue Zhi-Quan , Yang Zhen-Yu , Chai Zhi , Niu Jing-Ping , Shi Zhi-Yong
Published: February 2021

Purpose

The application of excessive chemical fertilizers during the cultivation of Astragalus membranaceus leads to a decline in the quality of this medicinal plant as well as the soil’s sustainable productivity. In this study, we developed a special microbial organic fertilizer for A. membranaceus and investigated its effects on plant growth and rhizosphere microbial communities.

Methods

The root biomass and main active components of A. membranaceus in different growth stages were measured to assess the impacts of microbial organic manure on plant growth. Meanwhile, 16S rRNA and ITS1 amplicons were amplified and high-throughput sequencing was performed to detect the dynamic impacts of microbial organic manure on rhizosphere microbial communities.

Result

The results demonstrated that microbial organic manure significantly increased wet and dry weights of A. membranaceus seedlings and the accumulation of two effective components (flavonoids and saponin) in bacterial fertilizer treatment groups are significant higher than the control group. Research on rhizosphere microbial flora shows that the number and polymorphism of bacteria and fungi were decreased after the application of special fertilizer during the rapid growth period of plant and then gradually increased with seedling growth. The community structure of bacteria was regulated after the application of special fertilizer, and the beneficial bacteria for plant growth are enriched. Functional profiles prediction showed that significant shifts in metabolic functions impacting KEGG pathways of the microbial fertilizer treatment groups are related to metabolism and biosynthesis.

Conclusion

The results indicate that the microbial organic manure can improve A. membranaceus growth by providing appropriate nutrients and regulating the rhizosphere microbial community which has good potential in ecological cultivation of A. membranaceus.
Human fetal liver MSCs are more effective than adult bone marrow MSCs for their immunosuppressive, immunomodulatory, and Foxp3+ T reg induction capacity
Authors: Yu Yi , Valderrama Alejandra Vargas , Han Zhongchao , Uzan Georges , Naserian Sina , Oberlin Estelle
Published: February 2021

Background

Mesenchymal stem cells (MSCs) exhibit active abilities to suppress or modulate deleterious immune responses by various molecular mechanisms. These cells are the subject of major translational efforts as cellular therapies for immune-related diseases and transplantations. Plenty of preclinical studies and clinical trials employing MSCs have shown promising safety and efficacy outcomes and also shed light on the modifications in the frequency and function of regulatory T cells (T regs). Nevertheless, the mechanisms underlying these observations are not well known. Direct cell contact, soluble factor production, and turning antigen-presenting cells into tolerogenic phenotypes, have been proposed to be among possible mechanisms by which MSCs produce an immunomodulatory environment for T reg expansion and activity. We and others demonstrated that adult bone marrow (BM)-MSCs suppress adaptive immune responses directly by inhibiting the proliferation of CD4+ helper and CD8+ cytotoxic T cells but also indirectly through the induction of T regs. In parallel, we demonstrated that fetal liver (FL)-MSCs demonstrates much longer-lasting immunomodulatory properties compared to BM-MSCs, by inhibiting directly the proliferation and activation of CD4+ and CD8+ T cells. Therefore, we investigated if FL-MSCs exert their strong immunosuppressive effect also indirectly through induction of T regs.

Methods

MSCs were obtained from FL and adult BM and characterized according to their surface antigen expression, their multilineage differentiation, and their proliferation potential. Using different in vitro combinations, we performed co-cultures of FL- or BM-MSCs and murine CD3+CD25−T cells to investigate immunosuppressive effects of MSCs on T cells and to quantify their capacity to induce functional T regs.

Results

We demonstrated that although both types of MSC display similar cell surface phenotypic profile and differentiation capacity, FL-MSCs have significantly higher proliferative capacity and ability to suppress both CD4+ and CD8+ murine T cell proliferation and to modulate them towards less active phenotypes than adult BM-MSCs. Moreover, their substantial suppressive effect was associated with an outstanding increase of functional CD4+CD25+Foxp3+ T regs compared to BM-MSCs.

Conclusions

These results highlight the immunosuppressive activity of FL-MSCs on T cells and show for the first time that one of the main immunoregulatory mechanisms of FL-MSCs passes through active and functional T reg induction.
The role and mechanism of mitochondrial functions and energy metabolism in the function regulation of the mesenchymal stem cells
Authors: Yan Wanhao , Diao Shu , Fan Zhipeng
Published: February 2021

Mesenchymal stem cells (MSCs) are multipotent cells that show self-renewal, multi-directional differentiation, and paracrine and immune regulation. As a result of these properties, the MSCs have great clinical application prospects, especially in the regeneration of injured tissues, functional reconstruction, and cell therapy. However, the transplanted MSCs are prone to ageing and apoptosis and have a difficult to control direction differentiation. Therefore, it is necessary to effectively regulate the functions of the MSCs to promote their desired effects. In recent years, it has been found that mitochondria, the main organelles responsible for energy metabolism and adenosine triphosphate production in cells, play a key role in regulating different functions of the MSCs through various mechanisms. Thus, mitochondria could act as effective targets for regulating and promoting the functions of the MSCs. In this review, we discuss the research status and current understanding of the role and mechanism of mitochondrial energy metabolism, morphology, transfer modes, and dynamics on MSC functions.
Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse
Authors: Benavente-Diaz Maria , Comai Glenda , Di Girolamo Daniela , Langa Francina , Tajbakhsh Shahragim
Published: February 2021

Background

Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered by the lack of a sensitive reporter to dynamically track cells during differentiation.

Results

Here, we report a knock-in mouse line expressing the tdTOMATO fluorescent protein from the endogenous Myogenin locus. Expression of tdTOMATO in MyogntdTom mice recapitulated endogenous Myogenin expression during embryonic muscle formation and adult regeneration and enabled the isolation of the MYOGENIN+ cell population. We also show that tdTOMATO fluorescence allows tracking of differentiating myoblasts in vitro and by intravital imaging in vivo. Lastly, we monitored by live imaging the cell division dynamics of differentiating myoblasts in vitro and showed that a fraction of the MYOGENIN+ population can undergo one round of cell division, albeit at a much lower frequency than MYOGENIN− myoblasts.

Conclusions

We expect that this reporter mouse will be a valuable resource for researchers investigating skeletal muscle biology in developmental and adult contexts.
Two CD9 tetraspanin family members of Japanese flounder (Paralichthys olivaceus): characterization and comparative analysis of the anti-infectious immune function
Authors: He Jiaojiao , Gu Hanjie , Wang Wenqi , Hu Yonghua
Published: February 2021

CD9 is a glycoprotein of the transmembrane 4 superfamily that is involved in various cellular processes. Studies related to the immune functions and activities of CD9 in teleost fish are limited. In this study, we characterized two CD9 homologs, PoCD9.1 and PoCD9.3, from Japanese flounder (Paralichthys olivaceus). Sequence analysis showed that PoCD9.1 and PoCD9.3 possess characteristic transmembrane 4 superfamily (TM4SF) structures. PoCD9.1 shares 70.61% sequence identity with PoCD9.3. The expression of PoCD9.1 and PoCD9.3 in the three main immune tissues was significantly induced in a time-dependent manner by extracellular and intracellular pathogen infection, which indicates that the two CD9 homologs play an important role in the response to pathogenic infection. Following infection with the extracellular pathogen Vibrio anguillarum, the expression profiles of both PoCD9.1 and PoCD9.3 were similar. After infection with the intracellular pathogen Edwardsiella piscicida, the expression levels of PoCD9.1 and PoCD9.3 were different at different stages of infection, especially in the spleen. The spleen was the most important tissue for the PoCD9.1 and PoCD9.3 responses to pathogen infection among the three examined immune tissues. Knockdown of PoCD9.1 and PoCD9.3 attenuated the ability of host cells to eliminate pathogenic bacteria, and PoCD9.1 knockdown was more lethal than PoCD9.3 knockdown for host cells with E. piscicida infection. Overexpression of PoCD9.1 and PoCD9.3 promoted host or host cell defence against E. piscicida infection. These findings suggest that PoCD9.1 and PoCD9.3 serve as immune-related factors, play an important role in the immune defence system of Japanese flounder, and display different functions in response to different pathogens at different stages of infection.
Adaptive offloading in mobile-edge computing for ultra-dense cellular networks based on genetic algorithm
Authors: Liao Zhuofan , Peng Jingsheng , Xiong Bing , Huang Jiawei
Published: February 2021

With the combination of Mobile Edge Computing (MEC) and the next generation cellular networks, computation requests from end devices can be offloaded promptly and accurately by edge servers equipped on Base Stations (BSs). However, due to the densified heterogeneous deployment of BSs, the end device may be covered by more than one BS, which brings new challenges for offloading decision, that is whether and where to offload computing tasks for low latency and energy cost. This paper formulates a multi-user-to-multi-servers (MUMS) edge computing problem in ultra-dense cellular networks. The MUMS problem is divided and conquered by two phases, which are server selection and offloading decision. For the server selection phases, mobile users are grouped to one BS considering both physical distance and workload. After the grouping, the original problem is divided into parallel multi-user-to-one-server offloading decision subproblems. To get fast and near-optimal solutions for these subproblems, a distributed offloading strategy based on a binary-coded genetic algorithm is designed to get an adaptive offloading decision. Convergence analysis of the genetic algorithm is given and extensive simulations show that the proposed strategy significantly reduces the average latency and energy consumption of mobile devices. Compared with the state-of-the-art offloading researches, our strategy reduces the average delay by 56% and total energy consumption by 14% in the ultra-dense cellular networks.
Laparoscopic removal of an aberrant acupuncture needle in the gluteus that reached the pelvic cavity: a case report
Authors: Yamamoto Akira , Hiro Junichiro , Omura Yusuke , Ichikawa Takashi , Ide Shozo , Imaoka Hiroki , Yasuda Hiromi , Fujikawa Hiroyuki , Okita Yoshiki , Yokoe Takeshi , Ohi Masaki , Toiyama Yuji
Published: February 2021

Background

Intrapelvic aberrant needles are rare in clinical practice. Long-term foreign bodies in the abdominal cavity may form granulation tissue or an abscess, and may cause organ injury. Therefore, such foreign bodies need prompt removal.

Case presentation

A 26-year-old male athlete was referred to our hospital for investigation of an aberrant acupuncture needle in the gluteus. The needle was unable to be removed during acupuncture treatment, and the end broke off and remained in the gluteus. Abdominal X-ray examination showed a thin, 40-mm-long, metallic foreign body resembling an acupuncture needle. Abdominal computed tomography showed an abnormal shadow in the gluteus. However, it was unclear whether the tip of the needle reached the pelvic cavity. Thus, it was decided to surgically extract the needle via laparoscopic surgery under X-ray guidance as a safe and minimally invasive method. Although X-ray fluoroscopy confirmed that the aberrant needle was located in the gluteus, the needle could not be felt with the forceps, as the peritoneum surrounding the needle had granulomatous changes due to inflammation. Therefore, the retroperitoneum was further dissected to search for the needle. Once the needle was identified, its flexibility enabled it to be easily removed by grasping it directly with a needle holder. The length of the aberrant needle was 40 mm. The postoperative course was uneventful, and the patient was discharged from hospital on postoperative day 2.

Conclusions

When a foreign body remains in the gluteus and its tip touches intrapelvic organs, such as the rectum, it is critical to determine the best approach for its safe removal. Given the anatomical location of the foreign body and the patient background, laparoscopic removal was considered the best approach in the present case.
Performance comparison of four types of target enrichment baits for exome DNA sequencing
Authors: Zhou Juan , Zhang Mancang , Li Xiaoqi , Wang Zhuo , Pan Dun , Shi Yongyong
Published: February 2021

Background

Next-generation sequencing technology is developing rapidly and target capture sequencing has become an important technique. Several different platforms for library preparation and target capture with different bait types respectively are commercially available. Here we compare the performance of the four platforms with different bait types to find out their advantages and limitations. The purpose of this study is to help investigators and clinicians select the appropriate platform for their particular application and lay the foundation for the development of a better target capture platform for next-generation sequencing.

Results

We formulate capture efficiency as a novel parameter that can be used to better evaluations of specificity and coverage depth among the different capture platforms. Target coverage, capture efficiency, GC bias, AT Dropout, sensitivity in single nucleotide polymorphisms, small insertions and deletions detection, and the feature of each platform were compared for low input samples. In general, all platforms perform well and small differences among them are revealed. In our results, RNA baits have stronger binding power than DNA baits, and with ultra deep sequencing, double stranded RNA baits perform better than single stranded RNA baits in all aspects. DNA baits got better performance in the region with high GC content and RNA baits got lower AT dropout suggesting that the binding power is different between DNA and RNA baits to genome regions with different characteristics.

Conclusions

The platforms with double stranded RNA baits have the most balanced capture performance. Our results show the key differences in performance among the four updated platforms with four different bait types. The better performance of double stranded RNA bait with ultra deep sequencing suggests that it may improve the sensitivity of ultra low frequent mutation detection. In addition, we further propose that the mixed baits of double stranded RNA and single stranded DNA may improve target capture performance.
Suitability of α1-microglobulin reduction rate as a biomarker of removal efficiency of online hemodiafiltration: a retrospective cohort study
Authors: Sakurai Kenji , Hosoya Hiromi , Kurihara Yoshitaka , Saito Takeshi
Published: February 2021

Background

Online hemodiafiltration (OL-HDF), whether in pre-dilution OL-HDF (pre-HDF) or post-dilution OL-HDF (post-HDF), is conducted to efficiently remove low molecular weight proteins from the blood of patients requiring dialysis. β2-microglobulin (β2-MG) and α1-microglobulin (α1-MG) are used as biomarkers to evaluate removal efficiency of OL-HDF.

We aimed to evaluate the relationship between β2-MG and α1-MG reduction rates and the amount of albumin leakage. Furthermore, we statistically analyzed the relationship between the α1-MG reduction rate and α1-MG removal amount, and its suitability as a biomarker for evaluating the removal efficiency of OL-HDF.

Methods

We collected the results of regularly conducted routine evaluations to assess the efficiency of OL-HDF from cases of patients undergoing maintenance dialysis at our clinic from 2018 to 2019. Data on was collected on both pre-HDF and post-HDF sessions. β2-MG and α1-MG reduction rates were analyzed. Regression analysis on reduction rates showed a significant correlation between the α1-MG reduction rate and the α1-MG removal amount.

Results

We conducted 435 tests on OL-HDF efficiency in 87 cases undergoing maintenance dialysis at our clinic in 2018 and 2019. There were 80.7 ± 4.5% for the β2-MG reduction rate, 33.8 ± 9.4% for the α1-MG reduction rate, and 3.9 ± 1.8 g/s for the amount of albumin leakage. There was no correlation between the β2-MG reduction rate and the α1-MG reduction rate, or between the amount of albumin leakage and β2-MG reduction rate.

Conclusion

α1-MG reduction rate was found to correlate with its removal amount, demonstrating its suitability as a biomarker for evaluating the removal efficiency of OL-HDF.

Trial registration

Retrospectively registered.
Migrant’s remittance and investment financing nexus in Africa: Does investment climate matter?
Authors: Nwokolo Chinenye Ifeoma , Ogbuagu Matthew Ikechukwu , Isola Wakeel Atanda
Published: February 2021

Development economists have enjoined Africans to leverage on remittance as their main source of investment financing due to its constant and undisrupted inflows despite structural distortions and economic weaknesses compared to other sources of financial flows in recent time. This ignited the motivation for this study, to unveil the nexus between migrant’s remittance and investment financing and the modulating effect of the investment climate in this relation on a panel of 28 sub-Saharan African countries over the period 1995 to 2017. Using the panel autoregression distributive lagged estimation technique, the following empirical findings were established. First, the theoretical supposition underpinning the assumption in investment climate as a factor that motivates migrant’s remittance inflow to be channelled to investment received a clear empirical support. Second, it was established that the interaction between remittance and components of investment climate (government size and open market) enhanced the growth of private investment. Last, remittance is found to exert a positive effect on private investment in as much as the former does not exceed the threshold of 78%, above which private investment would decline off the quadratic curve. The study suggests the policy-makers channel deliberate efforts at improving not only the efficiency of the market, but government participation especially in the area of tax policy and fiscal financing.
A phytomass-inspired carbon and its importance as an antibacterial agent against human pathogens
Authors: Anvarsha N. , Kalyani P.
Published: February 2021

Background

Imprudent use of antimicrobial drugs has resulted in the microbial resistance among the known microbes and hence we foresee a pressing need towards the development of novel, low-cost, and high potent antimicrobials which should be munificent by nature. In the pursuit of the above, phosphoric acid activated low-cost carbon was produced from a renewable phytomass precursor viz., leaves of Vitex negundo L. plant and explored for its antibacterial efficacy against four human pathogens viz., S. aureus, S. pyogenes (Gram-positive bacteria), and E. coli, P. aeruginosa (Gram-negative bacteria) by adopting well diffusion method. Carbon yield, burn-off, phase purity, elemental composition, particle morphology, and surface functionalities have been studied by ultimate elemental analysis, X-ray diffractometry, elemental analysis, scanning electron microscopy, and Fourier transform infrared spectrophotometry respectively. Minimal inhibition concentration (MIC) was also followed. Plausible mechanism of killing the pathogens by the above activated carbon was also provided.

Results

Vitex negundo leaves derived activated carbon (VNLAC) was found to contain large number of O-, S- and N-containing surface groups which are supposedly responsible for bestowing antibacterial properties to the carbon derived from Vitex negundo leaves. It has emerged as a potential antibacterial agent for many Gram-negative as well as Gram-positive bacteria. The inhibition zone of mean diameters ranged from 9 to 25 mm against all the pathogens was significantly (p < 0.05) less than that of the control viz., ciprofloxacin. Thus, the fundamental experimental results may extend the limits of carbon sources but also the conventional idea of obtaining active carbon to apply in technologies where carbon is inevitable.

Conclusion

The work not only demonstrates the promising potential of VNLAC as an efficient antibacterial agent but also presents a feasible mechanism of action of removing pathogens. Vitex negundo-derived carbon may become a cheap substitute for cost-prohibitive drugs. The findings of the work illustrate an easy choice as an antibacterial for topical application at infected sites.
Features of apparent resistivity anomaly of controlled-source audio-frequency magnetotellurics and prospects of oil shale in the Tongchuan area of the southern Ordos Basin, China
Authors: Zhang Chunguan , Yuan Bingqiang , Li Yuhong , Zhang Qiang , Han Li
Published: February 2021

Four controlled-source audio-frequency magnetotelluric (CSAMT) profiles were conducted in early 2011 to investigate the distribution rules of oil shale in the Tongchuan area of the southern Ordos Basin. After terrain correction, we high-pass filtered the resulting images to prepare the 2D CSAMT apparent resistivity profiles for further analysis. Specifically, we have correlated the high-pass-filtered apparent resistivity anomalies to the distribution of oil shale seen in three available wells. The results reveal a close relationship between the oil shale distribution and the anomalously high-apparent-resistivity belt-like anomalies in the Tongchuan area. Our analysis indicates two prospective areas with different depths for oil shale within the study area.
We Cannot Say What the Human Is: The Problem of Anthropology in Adorno’s Philosophy of Art
Authors: Gilchrist Kylie
Published: February 2021

This article investigates a problem in Theodor W. Adorno’s thought: how can Adorno critique advanced capitalist societies for their dehumanizing tendencies while also refusing the possibility of defining the human? Motivating this inquiry is a renewed investigation of philosophical anthropology by thinkers like Axel Honneth and Jürgen Habermas, who explore positive theories of human limits and needs as the basis of social critique. As Adorno consistently refused to define the human on philosophical and political grounds, this article asks whether his work offers an unexamined alternative to philosophical anthropology’s revival. A reconstruction of Adorno’s position shows how Adorno displaces anthropological problems into his philosophy of art, where the principle of mimesis offers a potentially nonanthropological model of human potential. Yet it also reveals how Adorno’s refusal to directly interrogate philosophical anthropology leads him to implicitly prescribe a certain figure of the human, undermining the value of his resistance to anthropological definitions.
The voltage-gated sodium channel β2 subunit associates with lipid rafts by S-palmitoylation
Authors: Cortada Eric , Serradesanferm Robert , Brugada Ramon , Verges Marcel
Published: February 2021

The voltage-gated sodium channel is critical for cardiomyocyte function. It consists of a protein complex comprising a pore-forming α subunit and associated β subunits. In polarized Madin–Darby canine kidney cells, we show evidence by acyl-biotin exchange that β2 is S-acylated at Cys-182. Interestingly, we found that palmitoylation increases β2 association with detergent-resistant membranes. β2 localizes exclusively to the apical surface. However, depletion of plasma membrane cholesterol, or blocking intracellular cholesterol transport, caused mislocalization of β2, as well as of the non-palmitoylable C182S mutant, to the basolateral domain. Apical β2 did not undergo endocytosis and displayed limited diffusion within the plane of the membrane; such behavior suggests that, at least in part, it is cytoskeleton anchored. Upon acute cholesterol depletion, its mobility was greatly reduced, and a slight reduction was also measured as a result of lack of palmitoylation, supporting β2 association with cholesterol-rich lipid rafts. Indeed, lipid raft labeling confirmed a partial overlap with apical β2. Although β2 palmitoylation was not required to promote surface localization of the α subunit, our data suggest that it is likely implicated in lipid raft association and the polarized localization of β2.
Microtubule-independent movement of the fission yeast nucleus
Authors: Ashraf Sanju , Tay Ye Dee , Kelly David A. , Sawin Kenneth E.
Published: February 2021

Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe, nuclear movement and positioning are thought to depend on microtubule polymerization-based pushing forces. Here, we describe a novel, microtubule-independent, form of nuclear movement in fission yeast. Microtubule-independent nuclear movement is directed towards growing cell tips, and it is strongest when the nucleus is close to a growing cell tip, and weakest when the nucleus is far from that tip. Microtubule-independent nuclear movement requires actin cables but does not depend on actin polymerization-based pushing or myosin V-based pulling forces. The vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) Scs2 and Scs22, which are critical for endoplasmic reticulum–plasma membrane contact sites in fission yeast, are also required for microtubule-independent nuclear movement. We also find that in cells in which microtubule-based pushing forces are present, disruption of actin cables leads to increased fluctuations in interphase nuclear positioning and subsequent altered septation. Our results suggest two non-exclusive mechanisms for microtubule-independent nuclear movement, which may help illuminate aspects of nuclear positioning in other cells.
Effects of wave-driven water flow on the fast-start escape response of juvenile coral reef damselfishes
Authors: Roche Dominique G.
Published: February 2021

Fish often evade predators with a fast-start escape response. Studies typically examine this behaviour in still water despite water motion being an inherent feature of aquatic ecosystems. In shallow habitats, waves create complex flows that likely influence escape performance, particularly in small fishes with low absolute swimming speeds relative to environmental flows. I examined how wave-driven water flow affects the behaviour and kinematics of escape responses in juveniles of three coral reef damselfishes (Pomacentridae) with different body morphologies. Tropical damselfishes have similar fin and body shapes during early development, with the exception of body depth, a trait deemed important for postural control and stability. Wave-driven flow increased response latency in two of the three species tested: fish with a fusiform body responded 2.9 times slower in wave-driven flow than in still water, whereas this difference was less pronounced in fish with an intermediate body depth (1.9 times slower response) and absent in fish with a laterally compressed body. The effect of wave-driven flow on swimming performance (cumulative escape distance and turning rate) was variable and depended on the timing and trajectory of escape responses in relation to the wave phase. Given intense predation pressure on juvenile coral reef fishes during settlement, interspecific differences in how wave-driven flow affects their ability to escape predators could influence the distribution and abundance of species across spatial and temporal scales.
Thresholds of polarization vision in octopuses
Authors: Temple Shelby E. , How Martin J. , Powell Samuel B. , Gruev Viktor , Marshall N. Justin , Roberts Nicholas W.
Published: February 2021

Polarization vision is widespread in nature, mainly among invertebrates, and is used for a range of tasks including navigation, habitat localization and communication. In marine environments, some species such as those from the Crustacea and Cephalopoda that are principally monochromatic, have evolved to use this adaptation to discriminate objects across the whole visual field, an ability similar to our own use of colour vision. The performance of these polarization vision systems varies, and the few cephalopod species tested so far have notably acute thresholds of discrimination. However, most studies to date have used artificial sources of polarized light that produce levels of polarization much higher than found in nature. In this study, the ability of octopuses to detect polarization contrasts varying in angle of polarization (AoP) was investigated over a range of different degrees of linear polarization (DoLP) to better judge their visual ability in more ecologically relevant conditions. The ‘just-noticeable-differences’ (JND) of AoP contrasts varied consistently with DoLP. These JND thresholds could be largely explained by their ‘polarization distance’, a neurophysical model that effectively calculates the level of activity in opposing horizontally and vertically oriented polarization channels in the cephalopod visual system. Imaging polarimetry from the animals’ natural environment was then used to illustrate the functional advantage that these polarization thresholds may confer in behaviourally relevant contexts.