bluuebunny/crossref_metadata_2025 huggingface dataset:
Solvent effect on the absorption and emission spectra of carbon dots: evaluation of ground and excited state dipole moment
Authors: Mohammad-Jafarieh Parisa , Akbarzadeh Abolfazl , Salamat-Ahangari Rahman , Pourhassan-Moghaddam Mohammad , Jamshidi-Ghaleh Kazem
Published: September 2021

Background

Carbon dots (C-dots) are photoluminescent nanoparticles with less than 10 nm in size. Today, many studies are performed to exploit the photoluminescence (PL) property of carbon dots, and our focus in this study is to estimate the dipole moment of carbon dots. For reaching our aims, C-dots were synthesized and dissolved in the different solvents.

Results

Carbon dots with intense photoluminescence properties have been synthesized by a one-step hydrothermal method from a carbon bio-source. In this research, we report on the effect of aprotic solvents on absorption and fluorescence spectra and dipole moments of C-dots dispersed in a range of many aprotic solvents with various polarity and dielectric constant at room temperature. The change in the value of dipole moment was estimated by using the Stokes shifts. The difference between the dipole moment of the excited state and the ground state was shown using an extended form of Lippert equations by Kawski and co-workers.

Conclusions

The values found for μg = 1.077 D, and μe = 3.157 D, as well as the change in the dipole moments. The results showed that the dipole moment of the excited state is more than the ground state, indicating a high density and redistribution of electrons in the excited state. Finally, the quantum yield of C-dots in the eclectic aprotic solvents was communicated and discussed.
Alcohol dehydrogenases AdhE and AdhB with broad substrate ranges are important enzymes for organic acid reduction in Thermoanaerobacter sp. strain X514
Authors: Hitschler Lisa , Nissen Laura Sofie , Kuntz Michelle , Basen Mirko
Published: September 2021

Background

The industrial production of various alcohols from organic carbon compounds may be performed at high rates and with a low risk of contamination using thermophilic microorganisms as whole-cell catalysts. Thermoanaerobacter species that thrive around 50–75 °C not only perform fermentation of sugars to alcohols, but some also utilize different organic acids as electron acceptors, reducing them to their corresponding alcohols.

Results

We purified AdhE as the major NADH- and AdhB as the major NADPH-dependent alcohol dehydrogenase (ADH) from the cell extract of the organic acid-reducing Thermoanaerobacter sp. strain X514. Both enzymes were present in high amounts during growth on glucose with and without isobutyrate, had broad substrate spectra including different aldehydes, with high affinities (< 1 mM) for acetaldehyde and for NADH (AdhE) or NADPH (AdhB). Both enzymes were highly thermostable at the physiological temperature of alcohol production. In addition to AdhE and AdhB, we identified two abundant AdhA-type ADHs based on their genes, which were recombinantly produced and biochemically characterized. The other five ADHs encoded in the genome were only expressed at low levels.

Conclusions

According to their biochemical and kinetic properties, AdhE and AdhB are most important for ethanol formation from sugar and reduction of organic acids to alcohols, while the role of the two AdhA-type enzymes is less clear. AdhE is the only abundant aldehyde dehydrogenase for the acetyl-CoA reduction to aldehydes, however, acid reduction may also proceed directly by aldehyde:ferredoxin oxidoreductase. The role of the latter in bio-alcohol formation from sugar and in organic acid reduction needs to be elucidated in future studies.
Metabolic engineering of the oleaginous yeast Yarrowia lipolytica PO1f for production of erythritol from glycerol
Authors: Jagtap Sujit Sadashiv , Bedekar Ashwini Ashok , Singh Vijay , Jin Yong-Su , Rao Christopher V.
Published: September 2021

Background

Sugar alcohols are widely used as low-calorie sweeteners in the food and pharmaceutical industries. They can also be transformed into platform chemicals. Yarrowia lipolytica, an oleaginous yeast, is a promising host for producing many sugar alcohols. In this work, we tested whether heterologous expression of a recently identified sugar alcohol phosphatase (PYP) from Saccharomyces cerevisiae would increase sugar alcohol production in Y. lipolytica.

Results

Y. lipolytica was found natively to produce erythritol, mannitol, and arabitol during growth on glucose, fructose, mannose, and glycerol. Osmotic stress is known to increase sugar alcohol production, and was found to significantly increase erythritol production during growth on glycerol. To better understand erythritol production from glycerol, since it was the most promising sugar alcohol, we measured the expression of key genes and intracellular metabolites. Osmotic stress increased the expression of several key genes in the glycerol catabolic pathway and the pentose phosphate pathway. Analysis of intracellular metabolites revealed that amino acids, sugar alcohols, and polyamines are produced at higher levels in response to osmotic stress. Heterologous overexpression of the sugar alcohol phosphatase increased erythritol production and glycerol utilization in Y. lipolytica. We further increased erythritol production by increasing the expression of native glycerol kinase (GK), and transketolase (TKL). This strain was able to produce 27.5 ± 0.7 g/L erythritol from glycerol during batch growth and 58.8 ± 1.68 g/L erythritol during fed-batch growth in shake-flasks experiments. In addition, the glycerol utilization was increased by 2.5-fold. We were also able to demonstrate that this strain efficiently produces erythritol from crude glycerol, a major byproduct of the biodiesel production.

Conclusions

We demonstrated the application of a promising enzyme for increasing erythritol production in Y. lipolytica. We were further able to boost production by combining the expression of this enzyme with other approaches known to increase erythritol production in Y. lipolytica. This suggest that this new enzyme provides an orthogonal route for boosting production and can be stacked with existing designs known to increase sugar alcohol production in yeast such as Y. lipolytica. Collectively, this work establishes a new route for increasing sugar alcohol production and further develops Y. lipolytica as a promising host for erythritol production from cheap substrates such as glycerol.
Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13
Authors: Ma Jiangshan , Li Qiang , Wu Yujie , Yue Huimin , Zhang Yanghong , Zhang Jiashun , Shi Muling , Wang Sixian , Liu Gao-Qiang
Published: September 2021

Background

Lignin is a complex aromatic heteropolymer comprising 15–30% dry weight of the lignocellulose. The complex structural characteristic of lignin renders it difficult for value-added utilization. Exploring efficient lignin-degrading microorganisms and investigating their lignin-degradation mechanisms would be beneficial for promoting lignin valorization. In this study, a newly isolated white-rot basidiomycete, Trametes hirsuta X-13, with capacity to utilize alkaline lignin as the sole substrate was investigated.

Results

The analysis of the fermentation properties of T. hirsuta X-13 using alkaline lignin as the sole substrate, including the mycelial growth, activities of ligninolytic enzymes and the rates of lignin degradation and decolorization confirmed its great ligninolysis capacity. The maximum lignin degradation rate reached 39.8% after 11 days of T. hirsuta X-13 treatment, which was higher than that of reported fungi under the same condition. Fourier transform infrared spectrometry (FTIR), gas chromatography–mass spectrometry (GC–MS) scanning electron micrographs (SEM), two-dimensional heteronuclear single quantum coherence NMR analysis (2D-HSQC NMR) collaborated with pyrolysis gas chromatography–mass spectrometry (py-GC/MS) analyses proved that lignin structure was severely deconstructed along with amounts of monomer aromatics generated. Furthermore, according to those chemical analysis, in addition to canonical Cα–Cβ breakage, the cleavage of lignin interunit linkages of β–β might also occur by T. hirsuta X-13.

Conclusions

This study characterized a newly isolated white-rot basidiomycete T. hirsuta X-13 with impressive alkaline lignin degradation ability and provided mechanistic insight into its ligninolysis mechanism, which will be valuable for the development of lignin valorization strategies.
Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs
Authors: Gandasegui Javier , Grau-Pujol Berta , Cambra-Pelleja María , Escola Valdemiro , Demontis Maria Antonietta , Cossa Anelsio , Jamine José Carlos , Balaña-Fouce Rafael , van Lieshout Lisette , Muñoz José , Martínez-Valladares María ,   The Stopping Transmission of Intestinal Parasites (STOP) Project Consortium
Published: September 2021

Background

There is an urgent need for an extensive evaluation of benzimidazole efficacy in humans. In veterinary science, benzimidazole resistance has been mainly associated with three single-nucleotide polymorphisms (SNPs) in the isotype-1 β-tubulin gene. In this study, we optimized the stool sample processing methodology and resistance allele frequency assessment in Trichuris trichiura and Necator americanus anthelmintic-related SNPs by pyrosequencing, and standardized it for large-scale benzimidazole efficacy screening use.

Methods

Three different protocols for stool sample processing were compared in 19 T. trichiura-positive samples: fresh stool, egg concentration using metallic sieves with decreasing pore size, and egg concentration followed by flotation with saturated salt solution. Yield of each protocol was assessed by estimating the load of parasite DNA by real-time PCR. Then, we sequenced a DNA fragment of the β-tubulin gene containing the putative benzimidazole resistance SNPs in T. trichiura and N. americanus. Afterwards, resistant and susceptible-type plasmids were produced and mixed at different proportions, simulating different resistance levels. These mixtures were used to compare previously described pyrosequencing assays with processes newly designed by our own group. Once the stool sample processing and the pyrosequencing methodology was defined, the utility of the protocols was assessed by measuring the frequencies of putative resistance SNPs in 15 T. trichiura- and 15 N. americanus-positive stool samples.

Results

The highest DNA load was provided by egg concentration using metallic sieves with decreasing pore size. Sequencing information of the β-tubulin gene in Mozambican specimens was highly similar to the sequences previously reported, for T. trichiura and N. americanus, despite the origin of the sample. When we compared pyrosequencing assays using plasmids constructs, primers designed in this study provided the most accurate SNP frequencies. When pooled egg samples were analysed, none of resistant SNPs were observed in T. trichiura, whereas 17% of the resistant SNPs at codon 198 were found in one N. americanus sample.

Conclusions

We optimized the sample processing methodology and standardized pyrosequencing in soil-transmitted helminth (STH) pooled eggs. These protocols could be used in STH large-scale screenings or anthelmintic efficacy trials.

Graphical Abstract
Modelling bluetongue risk in Kazakhstan
Authors: Abdrakhmanov Sarsenbay K. , Beisembayev Kanatzhan K. , Sultanov Akmetzhan A. , Mukhanbetkaliyev Yersyn Y. , Kadyrov Ablaikhan S. , Ussenbayev Altay Y. , Zhakenova Aigerim Y. , Torgerson Paul R.
Published: September 2021

Background

Bluetongue is a serious disease of ruminants caused by the bluetongue virus (BTV). BTV is transmitted by biting midges (Culicoides spp.). Serological evidence from livestock and the presence of at least one competent vector species of Culicoides suggests that transmission of BTV is possible and may have occurred in Kazakhstan.

Methods

We estimated the risk of transmission using a mathematical model of the reproduction number R0 for bluetongue. This model depends on livestock density and climatic factors which affect vector density. Data on climate and livestock numbers from the 2466 local communities were used. This, together with previously published model parameters, was used to estimate R0 for each month of the year. We plotted the results on isopleth maps of Kazakhstan using interpolation to smooth the irregular data. We also mapped the estimated proportion of the population requiring vaccination to prevent outbreaks of bluetongue.

Results

The results suggest that transmission of bluetongue in Kazakhstan is not possible in the winter from October to March. Assuming there are vector-competent species of Culicoides endemic in Kazakhstan, then low levels of risk first appear in the south of Kazakhstan in April before spreading north and intensifying, reaching maximum levels in northern Kazakhstan in July. The risk declined in September and had disappeared by October.

Conclusion

These results should aid in surveillance efforts for the detection and control of bluetongue in Kazakhstan by indicating where and when outbreaks of bluetongue are most likely to occur. The results also indicate where vaccination efforts should be focussed to prevent outbreaks of disease.

Graphical abstract
Long-term efficacy, safety, and immunogenicity of the adalimumab biosimilar, PF-06410293, in patients with rheumatoid arthritis after switching from reference adalimumab (Humira®) or continuing biosimilar therapy: week 52–92 data from a randomized, double-blind, phase 3 trial
Authors: Fleischmann Roy M. , Alvarez Daniel F. , Bock Amy E. , Cronenberger Carol , Vranic Ivana , Zhang Wuyan , Alten Rieke
Published: September 2021

Background/objective

REFLECTIONS B538–02 is a randomized, double-blind comparative study of the adalimumab (ADL) biosimilar PF-06410293, (ADL-PF), and reference ADL sourced from the European Union (ADL-EU) in patients with active RA. Therapeutic equivalence was demonstrated based on ACR20 responses at week 12 (primary endpoint). We report long-term safety, immunogenicity, and efficacy of ADL-PF in patients who continued ADL-PF treatment throughout 78 weeks or who switched from ADL-EU to ADL-PF at week 26 or week 52.

Methods

Eligible patients (2010 ACR/EULAR RA diagnosis criteria for ≥ 4 months; inadequate response to MTX, ≤ 2 doses non-ADL biologic), stratified by geographic regions were initially randomized (1:1) in treatment period 1 (TP1) to ADL-PF or ADL-EU (40 mg subcutaneously, biweekly), both with MTX (10–25 mg/week). At week 26 (start of TP2), patients receiving ADL-EU were re-randomized to remain on ADL-EU or transition to ADL-PF for 26 weeks. At week 52 (start of TP3), all patients received open-label treatment with ADL-PF for 26 weeks and were followed after last treatment dose to week 92. To evaluate maintenance of response after switching or remaining on ADL-PF, ACR20, DAS28-4(CRP), and other measures of clinical response/remission were assessed through week 78 as secondary endpoints. Three groups were evaluated: biosimilar, week 26 switch, and week 52 switch.

Results

Overall, 507 patients participated in TP3. ACR20 response rates at week 52 were 88.4%, 88.2%, and 87.6% for the biosimilar, week 26, and week 52 switch groups, respectively. ACR20 response rates and DAS28-4(CRP) scores were sustained and comparable across groups in TP3. Incidence of treatment-emergent adverse events (AEs) during TP3 and follow-up was 42.6% (biosimilar), 37.0% (week 26 switch), and 50.8% (week 52 switch); 3 (0.6%) patients (all week 52 switch) reported treatment-related serious AEs. ADL-PF was generally well tolerated, with a comparable safety profile across groups. Overall, incidences of patients with anti-drug antibodies in TP3 and follow-up were comparable among groups (46.1%, 46.5%, and 54.2%, respectively).

Conclusions

There were no clinically meaningful differences in safety, immunogenicity, and efficacy for patients who were maintained on ADL-PF for 78 weeks and those who had switched from ADL-EU at week 26 or week 52.

Trial registration

ClinicalTrials.gov, NCT02480153. First posted on June 24, 2015; EU Clinical Trials Register; EudraCT number: 2014-000352-29. Start date, October 27, 2014
A quasi-experimental examination of weight-reducing dehydration practices in collegiate male rowers
Authors: Kelly Dayton J. , West Sarah L. , O’Keeffe Nathan , Brown Liana E.
Published: September 2021

Background

Lightweight rowers commonly utilize weight loss techniques over 24-h before competition to achieve the qualifying weight for racing. The objective was to investigate, using a quasi-experimental design, whether changes in weight resulting from dehydration practices are related to changes in proxies of bodily systems involved in rowing and whether these relationships depend on the dehydration technique used.

Methods

Twelve elite male rowers performed a power test, an incremental VO2max test, and a visuomotor battery following: weight loss via thermal exposure, weight loss via fluid abstinence and then thermal exposure, and no weight loss. The total percent body mass change (%BMC), %BMC attributable to thermal exposure, and %BMC attributable to fluid abstinence were used to predict performance variables.

Results

Fluid abstinence but not thermal exposure was related to a lower total wattage produced on a incremental VO2max test (b = 4261.51 W/1%BMC, 95%CI = 1502.68–7020.34), lower wattages required to elicit 2 mmol/L (b = 27.84 W/1%BMC, 95%CI = 14.69–40.99) and 4 mmol/L blood lactate (b = 20.45 W/1%BMC, 95%CI = 8.91–31.99), and slower movement time on a visuomotor task (b = -38.06 ms/1%BMC, 95%CI = -62.09–-14.03).

Conclusions

Dehydration related weight changes are associated with reductions in some proxies of bodily systems involved in rowing but depend on the dehydration technique used.
The circadian rest-activity pattern predicts cognitive decline among mild-moderate Alzheimer’s disease patients
Authors: Targa Adriano D. S. , Benítez Iván D. , Dakterzada Faridé , Fontenele-Araujo John , Minguez Olga , Zetterberg Henrik , Blennow Kaj , Barbé Ferran , Piñol-Ripoll Gerard
Published: September 2021

Background

Alterations in circadian rhythms are present in the presymptomatic stage of Alzheimer’s disease (AD), possibly contributing to its pathogenesis. However, it is unknown whether such alterations are associated with worse outcomes once individuals are diagnosed with symptomatic disease. We aimed to evaluate the association between the circadian rest-activity pattern and AD-related features in patients with mild-moderate AD.

Methods

We assessed the circadian rest-activity pattern of consecutive patients with mild-moderate AD through actigraphy for 14 days. Cerebrospinal fluid was obtained to determine the levels of important pathological markers including amyloid-beta protein (Aβ42), phosphorylated tau (P-tau), total tau (T-tau), and neurofilament light (NF-L). Neuropsychological evaluation was conducted at the beginning of the study and after 12 months of follow-up. Linear regression models were performed considering the global population and Aβ42+ patients only.

Results

The cohort included 100 patients with mild-moderate AD. The median age [p25;p75] was 76.0 [73.0;80.0] years and 63.0% were female. Older age (effect size [SE] of 0.324 [0.096]; p = 0.001) and male sex (0.780 [0.193]; p = 0.001) were associated with increased fragmentation and decreased synchronization of the rhythm, respectively. After adjusting for age, sex, and season of the year, increased levels of T-tau (effect size [95% CI] of 0.343 [0.139 to 0.547]; p = 0.001) and NF-L (0.444 [0.212 to 0.676]; p = 0.001) were associated with a higher amplitude of the rest-activity rhythm. Increased fragmentation of the rhythm at baseline was associated with greater cognitive decline after one year of follow-up independent of age, sex, T-tau/Aβ42 ratio, educational level, and season of the year (− 0.715 [− 1.272 to − 0.157]; p = 0.013). Similar findings were obtained considering only the Aβ42+ patients.

Conclusions

Our results suggest a potential role of the circadian rest-activity pattern in predicting the cognitive decline of patients with mild-moderate AD. Further studies are warranted to confirm these findings and to elucidate whether there is causality among the observed associations.
Atopic dermatitis and risk of autoimmune diseases: a systematic review and meta-analysis
Authors: Lu Zhiyu , Zeng Ni , Cheng Yuxin , Chen Yihe , Li Yueyue , Lu Qian , Xia Qingyue , Luo Dan
Published: September 2021

Background

Atopic dermatitis is the most common chronic inflammatory skin disease and presents a major public health burden worldwide. Recent observational studies revealed the potential association between atopic dermatitis with autoimmune disorders. However, there is no meta-analysis of the prevalence or incidence of autoimmune diseases in atopic dermatitis. Therefore, considering the potential clinical implications of these associations, we aimed to assess the risk of autoimmune diseases in patients with atopic dermatitis using this method.

Methods

PubMed, Embase, and Web of Science were searched from inception to October, 2020. Observational studies which provided estimate effects with 95% CI or raw data were included. The quality of selected studies was evaluated using the Newcastle–Ottawa Scale. Odds ratio and relative risks were pooled using a random effects model and expressed with 95% confidence intervals.

Results

Fourteen observational studies were included in this systematic review and meta-analysis. The random-effects meta-analysis of case–control and cross-sectional studies showed a significant association of atopic dermatitis with mutiple autoimmune diseases, including alopecia areata, celiac disease, Crohn’s disease, rheumatoid arthritis, systematic lupus erythematosus, ulcerative colitis and vitiligo. Furthermore, pooling of the results of cohort studies showed that patients with atopic dermatitis were more likely to develop these autoimmune diseases.

Conclusion

Our meta-analysis showed that patients with atopic dermatitis were at higher risk of multiple autoimmune diseases including alopecia areata, celiac disease, Crohn’s disease, rheumatoid arthritis, systematic lupus erythematosus, ulcerative colitis and vitiligo. It is important for early detection of the affected group so that timely management can be initiated. Dermatologists and allergists should be aware of the autoimmune diseases in patients with atopic dermatitis and develop interventions if necessary. Also, limited by the present research, we still require more large-scale studies to further establish the association between atopic dermatitis and autoimmune diseases.
A rare association in a patient with non-muscle invasive bladder cancer: ureteral fibroepithelial polyp and ipsilateral renal cell carcinoma: a case report
Authors: Akan Serkan , Ediz Caner
Published: September 2021

Background

Fibroepithelial polyps located in the ureter constitute 2–6% of all benign tumors in the urinary system. Distinguishing these lesions from transitional cell carcinoma is essential to avoid unnecessary nephroureterectomy.

Case presentation

A 59-year-old asymptomatic caucasian male patient was enrolled in follow-up for Ta low-grade transitional cell bladder cancer 4 years ago in our clinic. A suspicious, solid, contrast-enhancing mass 15 × 9 mm in diameter in the anteromedial mid-section of the left kidney, which was causing minimal washout and largely located in the parenchyma, was reported as renal cell carcinoma on computed tomography during routine controls. In the excretory phase, soft-tissue densities of approximately 30 mm in length, which were located in the distal part of the left ureter at a distance of 40 mm from the ureterovesical junction, extending towards the lumen suggested a urethral carcinoma. Urothelial lesion was reported as fibroepithelial polyp after histopathological examination. Partial nephrectomy for the mass, which was reported as renal cell carcinoma in the left kidney, was performed in the first postoperative month. Histopathological examination revealed Fuhrman grade 1 papillary type renal cell carcinoma. No recurrence was observed in the first year after treatment.

Conclusions

Although our patient had a bladder transitional cell carcinoma and a suspicious renal cell carcinoma mass of 15 mm in the ipsilateral kidney, the patient was safeguarded from unnecessary nephroureterectomy early on by cross-sectional and endoscopic imaging of the ureter.
Contrast agents for photoacoustic imaging: a review of stem cell tracking
Authors: James Soorya , Neuhaus Kai , Murphy Mary , Leahy Martin
Published: September 2021

With the advent of stem cell therapy for spinal cord injuries, stroke, burns, macular degeneration, heart diseases, diabetes, rheumatoid arthritis and osteoarthritis; the need to track the survival, migration pathways, spatial destination and differentiation of transplanted stem cells in a clinical setting has gained increased relevance. Indeed, getting regulatory approval to use these therapies in the clinic depends on biodistribution studies. Although optoacoustic imaging (OAI) or photoacoustic imaging can detect functional information of cell activities in real-time, the selection and application of suitable contrast agents is essential to achieve optimal sensitivity and contrast for sensing at clinically relevant depths and can even provide information about molecular activity. This review explores OAI methodologies in conjunction with the specific application of exogenous contrast agents in comparison to other imaging modalities and describes the properties of exogenous contrast agents for quantitative and qualitative monitoring of stem cells. Specific characteristics such as biocompatibility, the absorption coefficient, and surface functionalization are compared and how the labelling efficiency translates to both short and long-term visualization of mesenchymal stem cells is explored. An overview of novel properties of recently developed optoacoustic contrast agents and their capability to detect disease and recovery progression in clinical settings is provided which includes newly developed exogenous contrast agents to monitor stem cells in real-time for multimodal sensing.
IL-1β-primed mesenchymal stromal cells exert enhanced therapeutic effects to alleviate Chronic Prostatitis/Chronic Pelvic Pain Syndrome through systemic immunity
Authors: Liu Hanchao , Zhu Xinning , Cao Xiaohui , Chi Ani , Dai Jian , Wang Zhenqing , Deng Chunhua , Zhang Min
Published: September 2021

Background

Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) seriously affects patient health. Despite the elusiveness of innate therapeutic effects, mesenchymal stromal cells (MSCs) hold great promise for inflammation-related diseases. Recent evidence indicates that disease-specific inflammatory cytokines could enhance the therapeutic effects of MSCs.

Methods

By establishing a CP/CPPS mouse model and pretreating MSCs with the cytokine interleukin-1β (IL-1β), we studied the IL-1β-primed MSC immunoregulatory ability and targeted migration ability in vitro and in CP/CPPS mice.

Results

IL-1β levels significantly increased in the prostate tissue and serum of experimental autoimmune prostatitis (EAP) mice. Pretreatment with IL-1β enhanced the immunomodulatory potential and targeted migration of MSCs in vitro. Furthermore, intravenous infusion of IL-1β-primed MSCs dampened inflammation in prostate tissues and alleviated hyperalgesia in EAP mice. The infused MSCs inhibited monocyte infiltration and promoted regulatory T lymphocyte formation in prostate tissue, thus remodeling the local environment. Surprisingly, IL-1β-primed MSCs exhibited improved accumulation in the spleen but not in prostate tissue. Accordingly, infused MSCs reshaped systemic immunity by reducing the proportion of Ly6ChighCD11b+ monocytes and boosting the proportion of CD4+Foxp3+ regulatory T lymphocytes in the spleen and lung. Inflammatory chemokine (C–C motif) ligand 2 (CCL2) decreased through the downregulation of the NF-κB and JNK/MAPK pathways by inflammatory resolution via MSCs infusion to alleviate pain.

Conclusion

In summary, IL-1β-primed MSCs restored systemic immunologic homeostasis to alleviate CP/CPPS by modulating systemic immunity. These findings provide a novel strategy to boost the therapeutic effects of MSC-based therapy for CP/CPPS and reveal the essential role of systematic immunity in the treatment of CP/CPPS with MSC infusion.
Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects
Authors: Tam Wai Long , Freitas Mendes Luís , Chen Xike , Lesage Raphaëlle , Van Hoven Inge , Leysen Elke , Kerckhofs Greet , Bosmans Kathleen , Chai Yoke Chin , Yamashita Akihiro , Tsumaki Noriyuki , Geris Liesbet , Roberts Scott J. , Luyten Frank P.
Published: September 2021

Background

Bones have a remarkable capacity to heal upon fracture. Yet, in large defects or compromised conditions healing processes become impaired, resulting in delayed or non-union. Current therapeutic approaches often utilize autologous or allogeneic bone grafts for bone augmentation. However, limited availability of these tissues and lack of predictive biological response result in limitations for clinical demands. Tissue engineering using viable cell-based implants is a strategic approach to address these unmet medical needs.

Methods

Herein, the in vitro and in vivo cartilage and bone tissue formation potencies of human pluripotent stem cells were investigated. The induced pluripotent stem cells were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids. The tissue formation capacity of these organoids was then challenged in an ectopic and orthotopic bone formation model.

Results

The derived chondrocytes expressed similar levels of collagen type II as primary human articular chondrocytes and produced stable cartilage when implanted ectopically in vivo. Upon targeted promotion towards hypertrophy and priming with a proinflammatory mediator, the organoids mediated successful bridging of critical size long bone defects in immunocompromised mice.

Conclusions

These results highlight the promise of induced pluripotent stem cell technology for the creation of functional cartilage tissue intermediates that can be explored for novel bone healing strategies.
Interaction of Salmonella Gallinarum and Salmonella Enteritidis with peripheral leucocytes of hens with different laying performance
Authors: Sreekantapuram Sravya , Berens Christian , Barth Stefanie A. , Methner Ulrich , Berndt Angela
Published: September 2021

Salmonella enterica ssp. enterica serovars Enteritidis (SE) and Gallinarum (SG) cause different diseases in chickens. However, both are able to reach the blood stream where heterophils and monocytes are potentially able to phagocytose and kill the pathogens. Using an ex vivo chicken whole blood infection model, we compared the complex interactions of the differentially host-adapted SE and SG with immune cells in blood samples of two White Leghorn chicken lines showing different laying performance (WLA: high producer; R11: low producer). In order to examine the dynamic interaction between peripheral blood leucocytes and the Salmonella serovars, we performed flow cytometric analyses and survival assays measuring (i) leucocyte numbers, (ii) pathogen association with immune cells, (iii) Salmonella viability and (iv) immune gene transcription in infected whole blood over a four-hour co-culture period. Inoculation of blood from the two chicken lines with Salmonella led primarily to an interaction of the bacteria with monocytes, followed by heterophils and thrombocytes. We found higher proportions of monocytes associated with SE than with SG. In blood samples of high producing chickens, a decrease in the numbers of both heterophils and Salmonella was observed. The Salmonella challenge induced transcription of interleukin-8 (IL-8) which was more pronounced in SG- than SE-inoculated blood of R11. In conclusion, the stronger interaction of monocytes with SE than SG and the better survivability of Salmonella in blood of low-producer chickens shows that the host–pathogen interaction and the strength of the immune defence depend on both the Salmonella serovar and the chicken line.
RETRACTED ARTICLE: Intelligent electrical equipment fiber Bragg grating temperature measurement system
Authors: Peng Anhua , Liu Chengwen , Yang Le
Published: September 2021

Fiber Bragg gratings are widely used in electrical equipment monitoring, structural health monitoring, power system fault monitoring and other actual detection occasions due to the unique advantages of anti-electromagnetic interference, low fusion loss, high sensitivity, small size, and easy distributed measurement. Fiber grating has been widely used in actual detection occasions such as electrical equipment monitoring, structural health monitoring, and power system fault monitoring. The temperature measurement of the motor based on fiber grating sensing technology not only has strong anti-electromagnetic interference ability, but also can realize multi-point distributed temperature measurement. The research of fiber grating temperature measurement system for intelligent electrical equipment is of great significance. This article mainly studies the optical fiber grating temperature measurement system of intelligent electrical equipment. This paper designs the temperature measurement system architecture, including data acquisition layer, data monitoring layer and remote monitoring layer. Complete the system design from two aspects: hardware design and software design. In the hardware part, the functions of the temperature sensor module transmission system, signal demodulation system, PLC and host computer are analyzed. Use FTM3501 fiber temperature converter is used to realize the automatic electrical fiber grating. Experimental results show that the thermometer has alarm and display analysis functions. The measurement uncertainty is analyzed, and the uncertainty of the calibration result of the fiber Bragg grating temperature sensor is 0.0725 °C; the fault judgment time of the system in this paper does not exceed 4.73 s, while the fault judgment time of the control group is more than 4.73 s. Compared with the control group, it has a faster fault accuracy speed. The system has high measurement accuracy and good stability, can be applied to actual temperature measurement systems, and has certain practical value.
Performance analysis of congestion-aware secure broadcast channels
Authors: Arvanitaki Antonia , Pappas Nikolaos , Carlsson Niklas , Mohapatra Parthajit , Burdakov Oleg
Published: September 2021

Congestion-aware scheduling in case of downlink cellular communication has ignored the distribution of diverse content to different clients with heterogeneous secrecy requirements. Other possible application areas that encounter the preceding issue are secure offloading in mobile-edge computing, and vehicular communication. In this paper, we extend the work in Arvanitaki et al. (SN Comput Sci 1(1):53, 2019) by taking into consideration congestion and random access. Specifically, we study a two-user congestion-aware broadcast channel with heterogeneous traffic and different security requirements. We consider two randomized policies for selecting which packets to transmit, one is congestion-aware by taking into consideration the queue size, whereas the other one is congestion-agnostic. We analyse the throughput and the delay performance under two decoding schemes at the receivers, and provide insights into their relative security performance and into how congestion control at the queue holding confidential information can help decrease the average delay per packet. We show that the congestion-aware policy provides better delay, throughput, and secrecy performance for large arrival packet probabilities at the queue holding the confidential information. The derived results also take account of the self-interference caused at the receiver for whom confidential data is intended due to its full-duplex operation while jamming the communication at the other user. Finally, for two decoding schemes, we formulate our problems in terms of multi-objective optimization, which allows for finding a trade-off between the average packet delay for packets intended for the legitimate user and the throughput for the other user under congestion-aware policy.
Approximate solutions and Hyers–Ulam stability for a system of the coupled fractional thermostat control model via the generalized differential transform
Authors: Etemad Sina , Tellab Brahim , Alzabut Jehad , Rezapour Shahram , Abbas Mohamed Ibrahim
Published: September 2021

In this paper, we consider a new coupled system of fractional boundary value problems based on the thermostat control model. With the help of fixed point theory, we investigate the existence criterion of the solution to the given coupled system. This property is proved by using the Krasnoselskii’s fixed point theorem and its uniqueness is proved via the Banach principle for contractions. Further, the Hyers–Ulam stability of solutions is investigated. Then, we find the approximate solution of the coupled fractional thermostat control system by using a numerical technique called the generalized differential transform method. To show the consistency and validity of our theoretical results, we provide two illustrative examples.
Effects of topographic variability and forest attributes on fine-scale soil fertility in late-secondary succession of Atlantic Forest
Authors: Rodrigues Alice Cristina , Villa Pedro Manuel , Ferreira-Júnior Walnir Gomes , Schaefer Carlos Ernesto R. G. , Neri Andreza Viana
Published: September 2021

Background

Understanding how soil fertility changes due to topographical conditions and forest attributes is an essential premise for local-scale forest management practices. We evaluated the effects of topographic variables and forest attributes on soil fertility along a local topographical gradient in a Brazilian Atlantic Forest. We hypothesised that soil fertility is positively affected by topographic variability and forest attributes (structure and diversity). We used tree species richness, composition, abundance, and aboveground biomass as forest attributes. We analysed two 1-ha forest patches with contrasting topographical conditions. We used different linear mixed effects models (LMMs) to test the main effects of different forest attributes and topography variables on soil fertility.

Results

The results showed that higher topographic variability determines soil fertility along a fine-scale gradient. The first two axes of the PCA explained 66.8% of the variation in soil data, with the first axis (PCA1) explaining 49.6% of the variation in soil data and positively correlating with fertility-related soil properties. The second axis (PCA2) explained 17.2% of the variation in topographical data and positively correlated with convexity (the elevation of a plot minus the average elevation of all immediate neighbour plots) and elevation. Our best models showed that topographic variables (elevation and convexity) are the main predictors that affect fine-scale soil fertility.

Conclusions

Our study demonstrates that the topographic variability, mainly elevation and convexity, determines fine-scale soil fertility in an Atlantic Forest. These results advance our understanding that context-dependent conditions based on topography and soil properties have a high variability at a fine scale, which can influence variations in forest attributes (i.e., species distribution, diversity and structure of tree communities). In addition, the information generated in this research may be important for planning forest restoration activities (passive and active) based on the high variability of environmental variables at a fine scale.
High-Strength Reinforcing Steel Bars: Low Cycle Fatigue Behavior Using RGB Methodology
Authors: Egger Jorge E. , Rojas Fabian R. , Massone Leonardo M.
Published: September 2021

Low cycle fatigue life of high-strength reinforcing steel bars (ASTM A706 Grade 80), using photogrammetry by RGB methodology is evaluated. Fatigue tests are performed on specimens under constant axial displacement with total strain amplitudes ranging from 0.01 to 0.05. The experimental observations indicate that buckling of high-strength reinforcing bars results in a damaging degradation of their fatigue life performance as the slenderness ratio increases, including an early rebar failure as the total strain amplitude increases since it achieves the plastic range faster. In addition to this, the results show that the ratio of the ultimate tensile strength to yield strength satisfies the minimum of 1.25 specified in ASTM A706 for reinforcement. On the other hand, the RGB methodology indicates that the axial strains measured by photogrammetry provide more accurate data since the registered results by the traditional experimental setup do not detect second-order effects, such as slippage or lengthening of the specimens within the clamps. Moreover, the RGB filter is faster than digital image correlation (DIC) because the RGB methodology requires a fewer computational cost than DIC algorithms. The RGB methodology allows to reduce the total strain amplitude up to 45% compared to the results obtained by the traditional setup. Finally, models relating total strain amplitude with half-cycles to failure and total strain amplitude with total energy dissipated for multiple slenderness ratios (L/d of 5, 10, and 15) are obtained.
Protective chromosome 1q32 haplotypes mitigate risk for age-related macular degeneration associated with the CFH-CFHR5 and ARMS2/HTRA1 loci
Authors: Pappas Chris M. , Zouache Moussa A. , Matthews Stacie , Faust Caitlin D. , Hageman Jill L. , Williams Brandi L. , Richards Burt T. , Hageman Gregory S.
Published: September 2021

Background

Single-variant associations with age-related macular degeneration (AMD), one of the most prevalent causes of irreversible vision loss worldwide, have been studied extensively. However, because of a lack of refinement of these associations, there remains considerable ambiguity regarding what constitutes genetic risk and/or protection for this disease, and how genetic combinations affect this risk. In this study, we consider the two most common and strongly AMD-associated loci, the CFH-CFHR5 region on chromosome 1q32 (Chr1 locus) and ARMS2/HTRA1 gene on chromosome 10q26  (Chr10 locus).

Results

By refining associations within the CFH-CFHR5 locus, we show that all genetic protection against the development of AMD in this region is described by the combination of the amino acid-altering variant CFH I62V (rs800292) and genetic deletion of CFHR3/1. Haplotypes based on CFH I62V, a CFHR3/1 deletion tagging SNP and the risk variant CFH Y402H are associated with either risk, protection or neutrality for AMD and capture more than 99% of control- and case-associated chromosomes. We find that genetic combinations of CFH-CFHR5 haplotypes (diplotypes) strongly influence AMD susceptibility and that individuals with risk/protective diplotypes are substantially protected against the development of disease. Finally, we demonstrate that AMD risk in the ARMS2/HTRA1 locus is also mitigated by combinations of CFH-CFHR5 haplotypes, with Chr10 risk variants essentially neutralized by protective CFH-CFHR5 haplotypes.

Conclusions

Our study highlights the importance of considering protective CFH-CFHR5 haplotypes when assessing genetic susceptibility for AMD. It establishes a framework that describes the full spectrum of AMD susceptibility using an optimal set of single-nucleotide polymorphisms with known functional consequences. It also indicates that protective or preventive complement-directed therapies targeting AMD driven by CFH-CFHR5 risk haplotypes may also be effective when AMD is driven by ARMS2/HTRA1 risk variants.
Acute and subacute toxicity evaluation of hydroalcoholic extract from the stem bark of Bois Bande (Parinari campestris Aubl.1772) in rats
Authors: Sundaram Venkatesan , Mohammed Stephanie , Srinivasan M. R. , Johnson Jenelle , Suepaul Rod , Pargass Indira , John Chernell , Ramdhanie Danesha , Lallack Shiann , Daniel Esther , Gilkes Lester
Published: September 2021

Introduction

The bark of Bois Bande (Parinari campestris) is a popular aphrodisiac in the Caribbean that has been traditionally used for many years to restore sexual vitality, increase sperm count, and treat erectile dysfunction, without valid scientific data. Acute and 28-day subacute toxicity studies were conducted to evaluate the safety of the hydroalcoholic extract of P.campestris bark and to find a safe dose for human use in conventional medicine.

Methods

The acute toxicity study used a single oral dose of P.campestris extract at four separate doses, 5, 50, 300, and 2,000 mg/kg, and was seen for 14 days, while the subacute toxicity study used a daily oral dose of P.campestris extract at 3 different doses, 100, 300, and 1000 mg/kg/day for 28 days.

Results

The LD50 of P.campestris extract was found to be greater than 2000 mg/kg in the acute toxicity study. P.campestris extract did not show toxicity at 1000 mg/kg/day in subacute toxicity trial; NOAEL was 1000 mg/kg/day in rats. However, the body weight was increased in males.

Conclusion

In conclusion, 1000 mg/kg P.campestris extract can be considered safe and non-toxic in males.
Incidence of Injuries, Illness and Related Risk Factors in Cross-Country Marathon Mountain Biking Events: A Systematic Search and Review
Authors: Buchholtz Kim , Lambert Mike , Corten Lieselotte , Burgess Theresa L.
Published: September 2021

Background

Cycling is a popular global sport and method of transportation and a significant contributor to admissions to hospital emergency units following an injury. Mountain biking events present additional challenges with remote venues and isolated courses, for which on-site medical care is often provided, for both injury and illness occurring during races. National health data may not represent these unique events, and specific data on incidence of injury and illness in mountain biking events are essential. Therefore, the aim of this study was to review the available injury and illness literature, reporting methods and risk factors in cross-country mountain biking.

Methods

Search engines PubMed, Scopus, CINAHL (EBSCOhost), Scopus, PEDro and the Cochrane Library were systematically searched, and a grey literature search was performed. Narrative analyses of the types, severity and area of injuries and illness type and severity were performed as pooling of data was impossible due to insufficient high-quality studies with the same injury and illness definitions.

Results

Seven studies comprising 28,021 participants were included for analysis. Four to 71% of participants sustained an injury in a cross-country mountain bike event. Injuries to the skin were the most common, followed by bony injuries and concussion. Five to 47% of cyclists reported the onset of gastrointestinal symptoms post-event. The prevalence of illness during events ranged from 0.5 to 23.0%.

Conclusion

The injury and illness definitions were varied and prevented clear comparisons between studies. Injury and illness present a concern in cross-country marathon mountain biking and should be investigated further to provide the true burden of these during race events.

Registration: This protocol has been registered with PROSPERO International prospective register of systematic reviews (No: CRD42019134586).