bluuebunny/crossref_metadata_2025 huggingface dataset:
2,4-Dichlorophenoxyacetic Acid Induces Degeneration of mDA Neurons In Vitro
Authors: Russ Tamara , Enders Lennart , Zbiegly Julia M. , Potru Phani Sankar , Wurm Johannes , Spittau Björn
Published: October 2023

Background: Parkinson’s disease (PD) affects 1–2% of the population over the age of 60 and the majority of PD cases are sporadic, without any family history of the disease. Neuroinflammation driven by microglia has been shown to promote the progression of midbrain dopaminergic (mDA) neuron loss through the release of neurotoxic factors. Interestingly, the risk of developing PD is significantly higher in distinct occupations, such as farming and agriculture, and is linked to the use of pesticides and herbicides. Methods: The neurotoxic features of 2,4-Dichlorophenoxyacetic acid (2,4D) at concentrations of 10 µM and 1 mM were analyzed in two distinct E14 midbrain neuron culture systems and in primary microglia. Results: The application of 1 mM 2,4D resulted in mDA neuron loss in neuron-enriched cultures. Notably, 2,4D-induced neurotoxicity significantly increased in the presence of microglia in neuron-glia cultures, suggesting that microglia-mediated neurotoxicity could be one mechanism for progressive neuron loss in this in vitro setup. However, 2,4D alone was unable to trigger microglia reactivity. Conclusions: Taken together, we demonstrate that 2,4D is neurotoxic for mDA neurons and that the presence of glia cells enhances 2,4D-induced neuron death. These data support the role of 2,4D as a risk factor for the development and progression of PD and further suggest the involvement of microglia during 2,4D-induced mDA neuron loss.
Evaluating the Potential of Delta Radiomics for Assessing Tyrosine Kinase Inhibitor Treatment Response in Non-Small Cell Lung Cancer Patients
Authors: Wang Ting-Wei , Chao Heng-Sheng , Chiu Hwa-Yen , Lin Yi-Hui , Chen Hung-Chun , Lu Chia-Feng , Liao Chien-Yi , Lee Yen , Shiao Tsu-Hui , Chen Yuh-Min , Huang Jing-Wen , Wu Yu-Te
Published: October 2023

Our study aimed to harness the power of CT scans, observed over time, in predicting how lung adenocarcinoma patients might respond to a treatment known as EGFR-TKI. Analyzing scans from 322 advanced stage lung cancer patients, we identified distinct image-based patterns. By integrating these patterns with comprehensive clinical information, such as gene mutations and treatment regimens, our predictive capabilities were significantly enhanced. Interestingly, the precision of these predictions, particularly related to radiomics features, diminished when data from various centers were combined, suggesting that the approach requires standardization across facilities. This novel method offers a potential pathway to anticipate disease progression in lung adenocarcinoma patients treated with EGFR-TKI, laying the groundwork for more personalized treatments. To further validate this approach, extensive studies involving a larger cohort are pivotal.
Investigation of the Relationship between Sensory-Processing Skills and Motor Functions in Children with Cerebral Palsy
Authors: Erkek Serhat , Çekmece Çiğdem
Published: October 2023

The main purpose of this study is to examine the relationship between sensory-processing skills and gross motor functions, bimanual motor functions, and balance in children with cerebral palsy (CP). A total of 47 patients between the ages of 3 and 10, diagnosed with CP, who received or applied for treatment in our physical therapy and rehabilitation unit were included in the study. Sensory profiling (SP), assisting hand assessment (AHA), the Gross Motor Function Measure-66 (GMFM-66), and the Pediatric Berg Balance Scale (PBBS) were used in the evaluation of the children with CP who participated in the study. The Gross Motor Function Classification System (GMFCS) was used to classify the children based on functional abilities and limitations, and the Manual Ability Classification System (MACS) was used to classify the children based on manual dexterity. The SP parameters were compared with AHA, GMFM-66, and PBBS results, and with GMFCS and MACS levels. Statistically significant relationships were found between AHA and SP, PBBS, and SP and between GMFM-66 and SP (p < 0.05). Our study shows that there are some disorders in sensory processing in children with CP. We think that sensory evaluations should be included in the CP rehabilitation program.
Influence of Media Information Sources on Vaccine Uptake: The Full and Inconsistent Mediating Role of Vaccine Hesitancy
Authors: Recio-Román Almudena , Recio-Menéndez Manuel , Román-González María Victoria
Published: October 2023

Vaccine hesitancy is a significant public health concern, with numerous studies demonstrating its negative impact on immunization rates. One factor that can influence vaccine hesitancy is media coverage of vaccination. The media is a significant source of immunization information and can significantly shape people’s attitudes and behaviors toward vaccine uptake. Media influences vaccination positively or negatively. Accurate coverage of the benefits and effectiveness of vaccination can encourage uptake, while coverage of safety concerns or misinformation may increase hesitancy. Our study investigated whether vaccine hesitancy acts as a mediator between information sources and vaccination uptake. We analyzed a cross-sectional online survey by the European Commission of 27,524 citizens from all EU member states between 15 and 29 March 2019. The study used structural equation modeling to conduct a mediation analysis, revealing that the influence of media on vaccine uptake is fully mediated by vaccine hesitancy, except for television, which depicted an inconsistent mediating role. In other words, the effect of different media on vaccine uptake is largely driven by the extent to which individuals are hesitant or resistant to vaccinating. Therefore, media outlets, governments, and public health organizations must work together to promote accurate and reliable information about vaccination and address vaccine hesitancy.
Exploring the Quotation Inertia in International Currency Markets
Authors: Musaev Alexander , Makshanov Andrey , Grigoriev Dmitry
Published: October 2023

The authors suggest a methodology that involves conducting a preliminary analysis of inertia in financial time series. Inertia here means the manifestation of some kind of long-term memory. Such effects may take place in complex processes of a stochastic kind. If the decision is negative, they do not recommend using predictive management strategies based on trend analysis. The study uses computational schemes to detect and confirm trends in financial market data. The effectiveness of these schemes is evaluated by analyzing the frequency of trend confirmation over different time intervals and with different levels of trend confirmation. Furthermore, the study highlights the limitations of using smoothed curves for trend analysis due to the lag in the dynamics of the curve, emphasizing the importance of considering real-time data in trend analysis for more accurate predictions.
The Impact of COVID-19 Pandemic on Acne Patients and Their Management: An Observational Multicenter Study from Italy
Authors: Grimaldi Marta , Micali Giuseppe , Bettoli Vincenzo , Odorici Giulia , Potenza Concetta , Musumeci Maria Letizia , Cacciapuoti Sara , Giovanardi Giulia , Agrifoglio Benedetta , Guerriero Cristina
Published: October 2023

Acne vulgaris was one of several diseases whose progression was significantly influenced psychologically by the rapid and protracted alteration of daily routines that occurred during the COVID-19 pandemic, especially for specific populations like adolescents. In order to assess their psychological impact and the relationship with the progression of acne vulgaris, this study aims to examine the quality of life, stress, anxiety, and depression of acne patients during the COVID-19 pandemic and their care. This observational, multicenter investigation was carried out in five dermatology clinics in Italy. Data were obtained via a remote telephone interview using questionnaires that were approved by a group of dermatologists and psychiatrists. Evaluations included demographic information, treatment status, disease progression, dietary habits, and employment activities. Some 178 acne vulgaris patients in various stages of systemic or topical medication were included in the study: 47 of 178 (26.4%) patients showed high scores on the HADS anxiety subscale, and 41 of 178 (23%) patients showed high scores on the HADS depression subscale. The Brief Resilience Scale (BRS) was used to measure resilience; 70 patients out of 178 (or 39.3%) showed low resilience (range: 1.00–2.99). In 32 out of 178 patients with PSS, high levels of stress were discovered (18%). Some 50 out of 178 patients experienced alterations in their way of life, including increased acne-related symptoms of relationship humiliation and constraints on everyday activities. Some 52 out of 178 patients (29.2%) said that they felt their condition had gotten worse over the study period. Our findings revealed a potential link between the exacerbation of acne and high levels of anxiety and depressive symptoms, as well as an elevated sense of stress and low or moderate levels of adaptability and resilience.
Progress and Perspectives in Colon Cancer Pathology, Diagnosis, and Treatments
Authors: Alrushaid Noor , Khan Firdos Alam , Al-Suhaimi Ebtesam , Elaissari Abdelhamid
Published: October 2023

Worldwide, colon cancer is the third most frequent malignancy and the second most common cause of death. Although it can strike anybody at any age, colon cancer mostly affects the elderly. Small, non-cancerous cell clusters inside the colon, commonly known as polyps, are typically where colon cancer growth starts. But over time, if left untreated, these benign polyps may develop into malignant tissues and develop into colon cancer. For the diagnosis of colon cancer, with routine inspection of the colon region for polyps, several techniques, including colonoscopy and cancer scanning, are used. In the case identifying the polyps in the colon area, efforts are being taken to surgically remove the polyps as quickly as possible before they become malignant. If the polyps become malignant, then colon cancer treatment strategies, such as surgery, chemotherapy, targeted therapy, and immunotherapy, are applied to the patients. Despite the recent improvements in diagnosis and prognosis, the treatment of colorectal cancer (CRC) remains a challenging task. The objective of this review was to discuss how CRC is initiated, and its various developmental stages, pathophysiology, and risk factors, and also to explore the current state of colorectal cancer diagnosis and treatment, as well as recent advancements in the field, such as new screening methods and targeted therapies. We examined the limitations of current methods and discussed the ongoing need for research and development in this area. While this topic may be serious and complex, we hope to engage and inform our audience on this important issue.
A Systematic Review of In Vitro Studies Using Microchip Platforms for Identifying Periodontopathogens from the Red Complex
Authors: Ardila Carlos M. , Jiménez-Arbeláez Gustavo A. , Vivares-Builes Annie Marcela
Published: October 2023

Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola, collectively recognized as periodontopathogens within the red complex, have been extensively studied in clinical samples collected from individuals with periodontitis. A lab-on-a-chip (LOC) is a miniature mechanism that integrates various laboratory operations onto a single microchip or a small-scale platform. This systematic review evaluates the application of LOC technology in identifying microorganisms from the red complex. This study adhered to PRISMA recommendations, and the review process encompassed several databases. In the electronic search, a total of 58 reports were found, and ultimately, 10 studies were considered relevant for inclusion. All these studies described effective, rapid, and reliable LOC systems for detecting and amplifying P. gingivalis, T. forsythia, and T. denticola. Compared to traditional methods, the LOC approach demonstrated minimal reagent requirements. Additionally, the results indicated that the amplification process took approximately 2 to 8 min, while detection could be completed in as little as 2 min and 40 s, resulting in a total experimental duration of around 11 min. Integrating miniaturization, speed, accuracy, and automation within microchip platforms makes them promising tools for detecting and amplifying microorganisms associated with the red complex in periodontal diseases.
Self-Authorship: A Pedagogical Tool for Pre-Service Teachers to Develop (Pre)Professional Identity
Authors: Botha Carolina , Marais Elma , Reyneke Maryna
Published: October 2023

This article reports on the use of self-authorship as a pedagogical tool to develop pre-service teachers’ professional identities. Pre-professional identity is considered a dynamic, less mature version of professional identity. Such a notion of fluidity in the professional self necessitates the integration of both personal and professional life experiences in the process of becoming, rather than already being, a teacher. A random sample of 56 pre-service teachers from a population of first-year students at a South African university was selected for this qualitative interpretivist study. Thematic analysis of personal reflections after a professional orientation programme indicates that the pre-professional identity of first-year pre-service teachers is mostly based on external cues and naïve perceptions rather than on well-thought-through personal ideology. The authors draw on Baxter-Magolda’s theory of self-authorship to highlight this influence of past life experiences that shape the pre-professional identity that first-year pre-service teachers bring to initial teacher training programs. Self-authorship is defined as a person’s ability to conceptualise and apply their own beliefs, identity, and social relations in various contexts. Findings confirm that most first-year pre-service teachers place themselves within the first phase of self-authorship. It is postulated that higher education institutions could, through platforms such as Work Integrated Learning, shift the structure and focus of pre-service teacher training programmes away from passive observation and instruction to active partnership, engaged reflection, and critical thinking. Such an approach can then contribute to professional and personal development through the remainder of the pre-service teacher programme. It is further argued that a longitudinal study is needed to explore this required movement towards and through the three phases of self-authorship.
The Influence of Protective Coatings on High-Temperature Corrosion under Biomass Ash Deposits
Authors: Maciejczyk Anna , Maj Izabella , Ciukaj Szymon , Hernik Bartłomiej , Osuch Arkadiusz
Published: October 2023

The presented research determined the performance of the protective coatings against the corrosion process under biomass and refuse-derived fuel (RDF) ash deposits. High-temperature corrosion tests were performed on steel grades intended for use in the fabrication of pressure parts working in elevated temperatures such as superheaters in power boilers. Two steel grades were investigated, P235GH and 16Mo3, and two protective coatings were applied, Inconel 625 and Alloy 310. The samples were sourced from the industrial boiler manufacturing company. The samples were exposed to two ashes originating from biofuels and one ash originating from RDF for comparison, all collected from Polish heat plants and power plants. Exposures were carried out in an oxidizing atmosphere for a maximum time of 504 h. The test temperatures of 480, 520, and 580 °C simulated superheater conditions of biofuel- and waste-fired power boilers. The corrosion kinetics were determined by weight change measurements conducted after 24, 168, and 504 h of the exposure. Regardless of the exposure temperature and the type of ash under which the samples were investigated, the use of 2.0 mm thick protective coatings resulted in smaller increases in the weight of the tested coupons which proves that alloy coatings give measurable results and are good remedies for chlorine-induced corrosion.
Transition to Sustainable Energy System for Smart Cities—Literature Review
Authors: Wyrwicka Magdalena Krystyna , Więcek-Janka Ewa , Brzeziński Łukasz
Published: October 2023

The article will contain a scientific analysis, showing thematic links between publications and consist of searching the data in the Scopus database. The timeframe of the searched publications will be 2010–2022. The parameters should also be narrowed down by selecting the following indexes: Science Citation Index Expanded (SCI-E), Social Science Citation Index (SSCI) and Emerging Sources Citation Index (ESCI), which was intended to eliminate abstracts of books and conference materials, leaving only reviewed works with the highest level of relevance for furthering knowledge. An additional limit of five minimum citations will be introduced. The analysis included 342 articles. Texts cited at least 100 times were highlighted. The research showed that authors focus piecemeal on selected aspects or problems, so an attempt was made to show thematic connections of keywords, illustrating the complexity of the transformations underway. The discussion identifies the most active authors and countries, especially exploring the topic of a transition to a sustainable energy system for smart cities. The authors have employed scientometric analysis to provide an objective and data-driven exploration of the transformation of sustainable energy systems for smart cities. This approach offers valuable insights into the research landscape, trends, and relationships within the field, which can guide future scientific research and contribute to a deeper understanding of the subject matter. As an additional element of this conclusion, the authors proposed supplementing the scientometric analysis with the foresight methodology. The authors’ research approach is distinguished by the following stages: problem formulation, data collection, choice of scientometric methodology, analysis of advantages and limitations of scientometrics, clustering analysis, data analysis, and presentation of results. Our systematic literature review systematizes the existing literature on the sustainable energy systems for smart cities, isolates main research interests, identifies future research avenues, and provides several important hints for researchers.
The Mechanical Response of Structural Elements in Enclosed Structures during Electric Vehicle Fires: A Computational Study
Authors: La Scala Armando , Loprieno Pierpaolo , Foti Dora , La Scala Massimo
Published: October 2023

Due to their reduced emissions and environmental benefits, electric vehicles (EVs) have grown in popularity over the past few years. However, EV fires can be a serious threat to nearby buildings, especially in garages where they are parked and charged. In this work, the behavior of the steel structural components in garages during electric car fires is computationally analyzed. To simulate the heat transmission and the structural reaction of a typical garage exposed to an EV fire, a finite element model was created. A comparison was made between the risk associated with fires generated by internal combustion vehicles (ICEVs) and EVs, with the elaboration of a risk index based on the deflections reached by a steel column under fire conditions. The model predictions are based on experimental data retrieved from various literature investigations, as well as regulatory simplified methods. The study conclusions provide information on how EV and ICEV fires affected garage performance, which may be used to design more resilient and safer buildings. The method represents a good compromise between the typical performance-based approaches and the tabular ones, characterized by good accuracy and low computational burden. This allows the professional to design optimized structures without wasting material and unnecessary coatings with their additional permanent loads, which could be detrimental in the case of other exceptional actions such as earthquakes.
A Study on the Lateral Load Capacity of a Novel Hybrid Monopile via a Centrifuge Model Test
Authors: Lee Ju-Hyung , Kwak Tae-Young , Jeong Youn-Ju , Park Joonsang , Kim Jae-Hyun
Published: October 2023

Large-diameter monopiles that can safely support the lateral loads caused by wind and waves have been widely used for the foundations of offshore wind turbines. However, when the penetration depth is insufficient as a result of the presence of thick soft ground or it is difficult to penetrate rock, the lateral load capacity of the monopiles may be insufficient, leading to structural instability of the wind turbine system. To address this problem, hybrid monopiles have been proposed, which include appendages such as suction buckets attached around the monopiles installed on the seabed. Such suction buckets are arranged in the form of a tripod at 120° intervals with respect to the center of the monopile. These increase the bending resistance of the monopile by sharing the lateral load applied to it. Although the proposed monopiles were presented as conceptual foundation types, their actual support mechanisms and bearing capacity improvement effects must be verified experimentally. In this study, a centrifuge model test was conducted to identify the support mechanism of hybrid monopiles and the degree of improvement in their bearing capacity compared to the existing large-diameter monopiles. The experiment results showed that an appendage composed of suction buckets dispersed the load acting on the monopile, thereby significantly increasing its bearing capacity.
Mathematical Modeling of Thin-Layer Drying Kinetics of Tomato Peels: Influence of Drying Temperature on the Energy Requirements and Extracts Quality
Authors: Popescu Mihaela , Iancu Petrica , Plesu Valentin , Bildea Costin Sorin , Manolache Fulvia Ancuta
Published: October 2023

Tomato drying implies high energy consumption due to the high moisture content, and limiting drying temperatures is necessary to avoid carotenoid degradation. To explain the mechanism of moisture transport through the material and to scale up the drying process, drying experiments are needed and supported by mathematical modeling. For the Rila tomato peel drying process, ten thin-layer mathematical models were formulated based on experimental data for six temperatures (50–75 °C) and validated by statistical analysis. Considering the slab geometry of the peels sample and Fick’s second law of diffusion model, the calculated effective moisture diffusivity coefficient values Deff varied between 1.01 × 10−9–1.53 × 10−9 m2/s with R2 higher than 0.9432. From the semi-theoretical models, Two-term presents the best prediction of moisture ratio with the highest R2 and lowest χ2 and RMSE values. Using the experimental data on extract quality (carotenoid content), two degradation models were formulated. Increasing the drying temperature from 50 °C to 110 °C, a degradation of 94% for lycopene and 83% for β-carotene were predicted. From the energy analysis, a specific energy consumption of 56.60 ± 0.51 kWh is necessary for hot-air drying of 1 kg of Rila tomato peel at 50 °C to avoid carotenoid degradation.
Composition and Anticoagulant Potential of Chondroitin Sulfate and Dermatan Sulfate from Inedible Parts of Garfish (Belone belone)
Authors: Chikha Sawssen Ben , Bougatef Hajer , Capitani Federica , Ben Amor Ikram , Maccari Francesca , Gargouri Jalel , Sila Assaad , Volpi Nicola , Bougatef Ali
Published: October 2023

Glycosaminoglycans (GAGs) play a crucial role due to their significant biomedical functions. Chondroitin sulfate (CS) and dermatan sulfate (DS), the main representative family of GAGs, were extracted and purified from garfish (Belone belone) by-products, i.e., skin (GSB), bones (GCB), and heads (GHB), and their composition and anticoagulant activity were investigated. CS/DS were purified by ion-exchange chromatography with yields of 8.1% for heads, 3.7% for skin, and 1.4% for bones. Cellulose acetate electrophoresis was also explored for analyzing the extracted CS/DS. Interestingly, GHB, GSB, and GCB possessed sulfate contents of 21 ± 2%, 20 ± 1%, and 20 ± 1.5%, respectively. Physico-chemical analysis showed that there were no significant differences (p > 0.05) between the variances for sulfate, uronic acid, and total sugars in the GAGs extracted from the different parts of fish. Disaccharide analysis by SAX-HPLC showed that the GSB and GCB were predominately composed of ΔDi-4S [ΔUA-GalNAc 6S] (74.78% and 69.22%, respectively) and ΔDi-2,4S [ΔUA2S-GalNAc 4S] (10.92% and 6.55%, respectively). However, the GHB consisted of 25.55% ΔDi-6S [ΔUA-GalNAc 6S] and 6.28% ΔDi-2,6S [ΔUA2S-GalNAc 4S]. Moreover, classical anticoagulation tests were also used to measure their anticoagulant properties in vitro, which included the activated partial thromboplastin time, prothrombin time, and thrombin time. The CS/DS isolated from garfish by-products exhibited potent anticoagulant effects. The purified CS/DS showed exceptional anticoagulant properties according to this research and can be considered as a new agent with anticoagulant properties.
The Influence of Probiotic Supplementation on the Obesity Indexes, Neuroinflammatory and Oxidative Stress Markers, Gut Microbial Diversity, and Working Memory in Obese Thai Children
Authors: Khongtan Suchanat , Sivamaruthi Bhagavathi , Thangaleela Subramanian , Kesika Periyanaina , Bharathi Muruganantham , Sirilun Sasithorn , Choeisoongnern Thiwanya , Peerajan Sartjin , Sittiprapaporn Phakkharawat , Chaiyasut Chaiyavat
Published: October 2023

Obesity is a worldwide health problem with a complex interaction between gut microbiota and cognition. Several studies have demonstrated that probiotic treatments improve characteristics linked to obesity. The present study aimed to evaluate the effects of probiotic supplementation on the obesity indexes, inflammatory and oxidative stress markers, gut microbiota, and working memory in obese children. Ten obese children were assigned to receive the probiotics (8 × 109 CFU of Lactobacillus paracasei HII01 and Bifidobacterium animalis subsp. lactis) for 12 weeks. Demographic data were recorded. Urine and fecal samples were collected to evaluate biomarkers related to obesity and cognition. Behavioral working memory was assessed using the visual n-back test. Electroencephalography was employed to measure electrical activity during the visual n-back test. All parameters were evaluated at the baseline and after 12 weeks. The results revealed that probiotic supplementation significantly altered some gut microbial metabolites, gut microbiota, total antioxidant capacity, and neuroinflammatory markers. However, no significant changes were observed in the visual n-back test or electroencephalographic recordings after 12 weeks. In conclusion, the use of probiotics might be an alternative treatment that could improve the gut microbial ecosystem and microbial metabolites, as well as host antioxidant and neuroinflammation levels. The preliminary results indicated that further detailed prolonged studies are needed in order to determine the beneficial effects of the studied probiotics.
Influence of Diet on the Bioavailability of Active Components from Zingiber officinale Using an In Vitro Digestion Model
Authors: Zagórska Justyna , Pietrzak Karolina , Kukula-Koch Wirginia , Czop Marcin , Laszuk Julia , Koch Wojciech
Published: October 2023

Ginger (Zingiber officinale Rosc.) is a plant known all over the world that is used as a spice and as an ingredient in drinks, dietary supplements, and cosmetics. The growing availability of its fresh rhizomes makes it even more likely to be used in the diet, mainly due to its beneficial health properties and high content of polyphenols (gingerols and shogaols). The main goal and motivation of the authors was to assess the bioavailability of active substances contained in the extract from ginger rhizomes in the presence of various types of diets using the in vitro digestion method, enabling simulation of the processes occurring during the digestion and absorption of metabolites in the small intestine. For the qualitative and quantitative analyses, the HPLC-MS (High Performance Liquid Chromatography–Mass Spectrometry) and HPLC (High Performance Liquid Chromatography) techniques were used, respectively. Based on the obtained results, it was found that the best bioavailability of the selected ginger polyphenols (6-gingerol, 8-gingerdione, 8-shogaol, and 10-gingerdione) was estimated for a high-fiber diet, while the weakest results were obtained for standard and basic diets. In the case of the high-fiber diet, the bioavailability of the mentioned compounds was estimated as 33.3, 21.4, 6.73, and 21.0%, while for the basic diet, it was only 21.3, 5.3, 2.0, and 1.0%, respectively.
High-Pressure Processing—Impacts on the Virulence and Antibiotic Resistance of Listeria monocytogenes Isolated from Food and Food Processing Environments
Authors: Wiśniewski Patryk , Chajęcka-Wierzchowska Wioleta , Zadernowska Anna
Published: October 2023

High-pressure processing (HPP) is one of the non-thermal methods of food preservation considered to be safe but may cause an increase/decrease in virulence potential and antibiotic resistance. The aim of the present study was to evaluate the survival of L. monocytogenes isolates after high-pressure processing (200 and 400 MPa for 5 min) and to determine changes in phenotypic and genotypic antibiotic resistance and virulence after this treatment. The 400 MPa treatment was shown to be effective in reducing pathogens to safe levels; however, the potential for cell recovery during storage was observed. In addition, studies on changes in virulence indicated possibilities related to a decrease in actA gene expression, overexpression of the hly and osfX gene, and an increase in biofilm-forming ability. The studies on changes in antibiotic resistance of isolates showed that all isolates showing initial susceptibility to lincomycin, fosfomycin, trimethoprim/sulfamethoxazole, and tetracycline became resistant to these antibiotics, which was associated with an increase in the values of minimum inhibitory concentrations. An increase in the expression of antibiotic resistance genes (mainly tetA_1, tetA_3, tetC) was also observed (mainly after the application of 200 MPa pressure), which was isolate dependent. However, it is noteworthy that the induced changes were permanent, i.e., they persisted even after the restoration of optimal environmental conditions. The results presented in our work indicate that the stress occurring during HPP can affect both phenotypic and genotypic changes in the virulence and antibiotic resistance potential of pathogens isolated from food and food processing environments. The potential associated with cell recovery and persistence of changes may influence the spread of virulent isolates of pathogens with increased antibiotic resistance in the food and food processing environment.
Investigating the Impact of Undulation Amplitude of Unconventional Oil Well Laterals on Transient Multiphase Flow Behavior: Experimental and Numerical Study
Authors: Khetib Youcef , Ling Kegang , Tang Clement , Aoun Ala Eddine , Fadairo Adesina Samson , Ouadi Habib
Published: October 2023

The growing popularity of unconventional wells has led to increased interest in assessing and predicting their production performance. These wells, with their extended-reach structures, are able to generate and access larger reservoir volumes. Therefore, understanding the impact of a well’s lateral trajectory on its transient production performance is crucial. This study investigates the effect of lateral-trajectory undulation amplitude on flow behavior based on the experimental results obtained at the University of North Dakota using an undulated two-phase (UTP) flow loop. The experiments involved injecting an air-and-water mixture through a section with variable undulation amplitude followed by a vertical section. The results revealed that the increasing undulation amplitude resulted in lower translational velocity, frequency, and length, with consistent slug acceleration along the system profile. Additionally, the frequency of slugs decreased as they traveled through the vertical section. The measured data indicated that higher undulation amplitudes led to increased horizontal pressure losses and variability, suggesting larger instabilities. The numerical simulations predicted lower translational velocity and frequency, longer slug length, and similar vertical pressure losses when compared to the experimental results.
Impacts of Complete Unemployment Rates Disaggregated by Reason and Duration on Suicide Mortality from 2009–2022 in Japan
Authors: Matsumoto Ryusuke , Motomura Eishi , Okada Motohiro
Published: October 2023

In Japan, suicides had consistently decreased before the COVID-19 pandemic (from 2009–2019), but conversely increased after the pandemic outbreak (from 2020–2022). To identify the features of fluctuations of suicides in Japan, the standardized suicide mortality rates per 100,000 population (SMRP) disaggregated by gender (males/females) and age (10-year cohorts) from 2009–2022 were analyzed using interrupted time-series and joinpoint regression analyses. Temporal causalities from unemployment rate (CUR) disaggregated by unemployment duration and reasons for seeking work to SMRP were analyzed using vector autoregressive modelling with Granger causality analysis. SMRP fluctuations from 2009–2022 were composed of three patterns, such as positive discontinuity (increasing) synchronized with the pandemic outbreak, attenuations of decreasing trends before the pandemic, turning from decreasing before the pandemic to increasing/unchanging after the pandemic outbreak. Dismissal CUR positively related to SMRP of working-age generations, whereas voluntary CUR negatively related to SMRP of younger population (<30 years), which turned to persistently increasing before the pandemic (approximately 2016–2017). CUR shorter than 3 months positively related to SMRP of working-age females, which displayed promptly increasing synchronization with the pandemic outbreak. CUR longer than 12 months positively related to SMRP of working-age males, which contributed to persistently increasing SMRPs during the pandemic. These results suggest that increasing SMRP during 2020–2022 in Japan has been probably at-tributed to interactions among the pandemic-related factors, continuous vulnerabilities from before the pandemic and newly developing risk factors for suicides during the pandemic. Unexpectedly, increasing SMRPs of working-age males in 2022 suggest that either prolongation of the pandemic or the ending of the pandemic might positively affect suicides in Japan.
Influences of Adenoid Hypertrophy on Children’s Maxillofacial Development
Authors: Lan Yulin , Chen Jieyi , Chen Shoucheng , He Yifan , Huang Fang
Published: October 2023

This study aims to investigate the association between adenoid hypertrophy and facial development. A total of 388 children aged 1–13 years old who had undergone head MRI in Foshan Maternal and Child Health Hospital were collected, including 196 hypertrophic cases and 192 normal cases. The maxillofacial soft tissue indicators were measured and compared. The A/N ratio and adenoid thickness consistently increased with age in the hypertrophic group and the A/N ratio reached a maximum value three years earlier than the normal group. The pharyngeal airway space, vallecula of epiglottis to anterior plane distance of the third/fourth cervical vertebrae, angle of convexity, total angle of convexity, and the nasolabial angle in the hypertrophy group were smaller than those in the control group (p < 0.05). The thickness of adenoids, palate height, palate length, and tongue length in the hypertrophy group exceeded that of the control group (p < 0.05). To conclude, adenoid hypertrophy was associated with craniofacial features such as a convex facial profile, a narrowed nasopharyngeal airway, an elongated and heightened palate, a lengthened tongue or a lower tongue position. These findings emphasize the importance of early intervention for children with adenoid hypertrophy to mitigate potential adverse effects on maxillofacial development.
Pride Medical at Capitol Hill: A New Lesbian, Gay, Bisexual, Transgender, Queer/Questioning plus (LGBTQ+) Patient Care Option at Kaiser Permanente Mid-Atlantic States
Authors: Jonas Mary Cabell , Egan Keith , Sheu Yi-Shin , McCarthy Richard J. , Horberg Michael A.
Published: October 2023

When accessing medical care, lesbian, gay, bisexual, transgender, queer/questioning plus (LGBTQ+) individuals face many known challenges, including stigma, discrimination, and health disparities. Transgender and nonbinary individuals often encounter physicians and staff who are not knowledgeable about gender-affirming services and the transition journey. Finding an affirming physician can be a trial-and-error process, causing concern and uncertainty. In 2021, Kaiser Permanente Mid-Atlantic States (KPMAS) researchers published a study examining the gaps in care and experience for transgender and nonbinary patients within the KPMAS healthcare system. KPMAS realized an opportunity to both close the gaps in care identified by transgender and nonbinary patients and enhance services for the broader LGBTQ+ patient community by creating Pride Medical at Capitol Hill—an additional and optional care site for individuals who identify as LGBTQ+. During the analysis timeframe of 30 June 2021 through 30 November 2022, 586 patients accessed care through 763 visits. A total of 675 visits (88%) were for primary care and 88 (12%) for OB/GYN. Over 50% (n = 384) of total visits were conducted virtually. The plurality of patients seen identified as a man (35%; n = 204) and gay (30%; n = 176). Postvisit survey results showed that 92% of survey respondents strongly agreed that the physician treated them with courtesy and respect, and 72% of survey respondents rated their overall care as excellent. Survey results show high acceptability of this program among the patients served. Pride Medical does not carve out care. The program offers patients access to a more specialized team of physicians—a similar model to other specialties—that is easily found by the division name Pride Medical. Layering additional specialty divisions on top of existing care, for interested patients, could be an option for other medical groups and health systems seeking to offer additional options of care for interested LGBTQ+ patients.
Exploring the Effect of Discrimination on Ethnic Minority Medical Students’ Mental Well-Being in the Netherlands
Authors: Anjorin Omolayo , Bakeroot Virginie L. , Zanting Albertine , Krumeich Anja , Busari Jamiu O.
Published: October 2023

Racial discrimination is a global concern affecting education, including medical programs in the Netherlands. Covert racial discrimination in academia has been linked to adverse academic outcomes and unequal opportunities. This qualitative study explores the relationship between racism, well-being, and academic success among ethnic minority students in Dutch medical education. Conducted in 2021 at a southern Netherlands university, this research employed interviews to delve into the racial discrimination experiences of 11 diverse medical students. Utilising a phenomenological approach, the study conducted semi-structured interviews and applied thematic content analysis to understand how racial discrimination impacted the students’ well-being. The findings revealed the prevalence of both overt and covert racial discrimination in predominantly white educational environments. Ethnic minority students encountered discrimination that negatively affected their well-being and academic performance. Racism manifested in overt and covert ways, with students often normalising exclusionary and racist situations. Ethnic minority students reported experiencing more racial discrimination than their white European peers, resulting in feelings of exclusion and academic challenges. The study suggests that implementing student-centred inclusivity measures through structural and institutional changes, such as support structures, mentoring programs, and inclusive course content, can mitigate racial discrimination, enhance diversity and inclusion, and improve ethnic minority medical students’ mental well-being and academic performance. Addressing covert racism is crucial for fostering an equitable and inclusive educational environment. These findings underscore the importance of proactive action to combat racial discrimination in educational settings.
Physico-Chemical Characterization, Phenolic Compound Extraction and Biological Activity of Grapevine (Vitis vinifera L.) Canes
Authors: Filimon Răzvan Vasile , Bunea Claudiu Ioan , Bora Florin Dumitru , Filimon Roxana Mihaela , Dunca Simona Isabela , Rózsa Sándor , Ciurlă Liliana , Patraș Antoanela
Published: October 2023

Annual grapevine pruning produces large amounts of unused waste as woody canes. The current study is aimed at the sustainable valorization of viticultural waste by establishing phenolic compound extraction conditions, composition, and biological potential of crude and purified cane extracts of three Vitis vinifera L. cultivars growing in temperate climate conditions. Grapevine canes proved to be rich in carbohydrates and minerals; chlorophyll and carotenoids were also quantified. The highest yield of phenolic compounds was obtained when dry canes (<0.5 mm) were subjected to liquid–solid extraction (1:20 w/v) with 70% (v/v) ethanol, for 4 h at 35 °C, after a preliminary ultrasound treatment (6 min., 42 KHz); Pinot Gris canes showing the highest concentration of flavonoids and non-flavonoids. Stilbenes (resveratrol) and flavan-3-ols (catechin and epicatechin) were the main phenolic representative, resveratrol concentrations varying significantly between red-black (419.01–425.60 μg/g d.w.) and white (282.19 ± 4.14 μg/g d.w.) grape cultivars. Purified extracts (C-18 cartridge) exhibited higher antioxidant and antiradical activities compared to the crude extracts, and a higher antimicrobial effect, especially against Gram-positive (Staphylococcus aureus) pathogenic bacteria. Furthermore, Saccharomyces cerevisiae and Oenococcus oeni showed a reduced susceptibility even at high extract concentrations (>100 mg/mL). These findings indicate that grapevine canes represent a valuable source of natural bioactive compounds, that are currently insufficiently known and not exploited to their true functional and economic potential.
Research on Time-Aware Group Query Method with Exclusion Keywords
Authors: Zhang Liping , Li Jing , Li Song
Published: October 2023

Aiming at the problem that the existing spatial keyword group query problem did not consider the query requirements with exclusion keywords and time attributes, a time-aware group query problem with exclusion keywords (TEGSKQ) is proposed for the first time. To solve this problem effectively, this paper proposes a query method based on the EKTIR-Tree index and dominating group (EKTDG). This method first proposes the EKTIR-tree index, which incorporates Huffman coding and integrates Bloom filters to deal with excluded keywords in order to improve the hit rate of keyword queries, significantly improving the query efficiency and reducing the storage occupancy. Then, the Candidate algorithm is proposed based on the EKTIR-tree index to filter out the spatial–textual objects that meet the query’s keywords and time requirements, narrowing the search space for subsequent queries on a large scale. To address the problem of the low efficiency of existing algorithms based on a spatial distance query, a distance-dominating group is defined and a pruning algorithm based on a spatial distance-dominating group is proposed, which is a refining process of query results and greatly improves the search efficiency of the query. Theoretical and experimental studies show that the proposed method can better handle group queries with exclusion keywords based on time awareness.
The Enolase of the Haemophilus influenzae Mediates Binding to Collagens: An Extracellular Matrix Component
Authors: Osorio-Aguilar Yesenia , Gonzalez-Vazquez Maria Cristina , Lozano-Zarain Patricia , Martinez-Laguna Ygnacio , Baylon-Pacheco Lidia , Rosales-Encina Jose Luis , Carabarin-Lima Alejandro , Rocha-Gracia Rosa del Carmen
Published: October 2023

Enolase proteins play a significant role as moonlighting proteins. In their role as surface-associated enolase, they have multiple functions as they interact with extracellular matrix proteins. Type I and III collagens are the major constituents of this extracellular matrix, and collagen is one of the targets of interaction with the enolase of many pathogens, thereby helping the colonization process and promoting the subsequent invasion of the host. This work aimed to determine the participation of non-typeable H. influenzae enolase as a collagen-binding protein. In this study, through the use of in vitro tests it was demonstrated that recombinant enolase of non-typeable H. influenzae (rNTHiENO) strongly binds to type I collagen. Using molecular docking, the residues that could take part in the interaction of non-typeable H. influenzae enolase-type I collagen (NTHiENO-Cln I) and non-typeable H. influenzae enolase-type III collagen (NTHiENO-Cln III) were identified. However, in vitro assays show that NTHiENO has a better affinity to interact with Cln I, concerning type Cln III. The interaction of NTHiENO with collagen could play a significant role in the colonization process; this would allow H. influenzae to increase its virulence factors and strengthen its pathogenesis.
Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis in Nicotiana attenuata
Authors: Waller Spenser , Powell Avery , Noel Randi , Schueller Michael J. , Ferrieri Richard A.
Published: October 2023

The effect of high-temperature (HT) stress on nicotine biosynthesis in Nicotiana attenuata was examined. Nicotine content was measured in mature leaves, young sink leaves, and in roots from well-watered plants grown at 25 °C as controls and from plants exposed to 38 °C and 43 °C temperatures applied for 24, 48, 72, and 96 h duration. At 38 °C, all leaf nicotine levels were significantly less than control plants for up to 72 h exposure but rose sharply thereafter to levels significantly greater than controls with 96 h exposure. In contrast, plants exposed to 43 °C never exhibited a reduction in leaf nicotine content and showed an increase in content with just 48 h exposure. Using radioactive 11CO2 and 13NO3−, we found that HT stress reduced both CO2 fixation and nitrate uptake. Furthermore, radiocarbon flux analysis revealed that ‘new’ carbon partitioning (as 11C) into the 11C-radiolabeled amino acid (AA) pool was significantly reduced with HT stress as were yields of [11C]-aspartic acid, an important AA in nicotine biosynthesis, and its beta-amido counterpart [11C]-asparagine. In contrast, [12C]-aspartic acid levels appeared unaffected at 38 °C but were elevated at 43 °C relative to controls. [12C]-Asparagine levels were noted to be elevated at both stress temperatures. Since HT reductions in carbon input and nitrogen uptake were noted to impede de novo AA biosynthesis, protein degradation at HT was examined as a source of AAs. Here, leaf total soluble protein (TSP) content was reduced 39% with long exposures to both stress temperatures. However, Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) which was 41% TSP appeared unaffected. Altogether, these results support the theory that plant proteins other than Rubisco degrade at elevated temperatures freeing up essential AAs in support of nicotine biosynthesis.