bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated transgene and transcriptome reveal the molecular basis of MdWRKY87 positively regulate adventitious rooting in apple rootstock
Authors: Tian Qiuye , Xu Mengli , Wu Dongchen , Wang Chaoping , Wang Xianlin , Che Qinqin , Li Zhengnan , Xu Xiaozhao
Published: January 2023

For most fruit and forest species vegetative propagated from elite genotypes, adventitious rooting is essential. The ability to form adventitious roots significantly decreased during the juvenile to adult phase change. Apart from the miR156-SPL pathway, whether there is another regulation mechanism controlling age-dependent adventitious rooting ability remained largely unknown. In the present study, we showed that MdWRKY87 expression level was positively correlation with adventitious rooting ability. In addition, over-expressing of MdWRKY87 in tobacco leads to enhanced adventitious rooting ability, more adventitious root number and accelerated adventitious rooting process. Comparative transcriptome profiling indicated that MdWRKY87 overexpression can activate the expression of adventitious rooting-induced genes, such as WOX11 and AIL. In addition, MdWRKY87 overexpression can inhibit the transcription of adventitious rooting-repressed genes, such as AUX/IAAs and type-B cytokinin RRs. Collectively, here we demonstrated that higher expression level of MdWRKY87 contributes to age-dependent adventitious rooting-competent in juvenile apple rootstock.
Sourcing practices of online news media in Switzerland during the war in Ukraine
Authors: Udris Linards , Vogler Daniel , Weston Morley , Eisenegger Mark
Published: January 2023

Introduction

In times of war, sourcing becomes a major challenge for journalists. Information is often unavailable because access is restricted or because reporting on the ground is prohibited, too dangerous, or even simply too costly for media having to work with limited resources. Which sources actually shape the news is a highly relevant question because news media still constitute the main channel of information for many citizens, especially when it comes to wars abroad. For a long time, scholars have diagnosed a dominance of official sources in war coverage but have not analyzed whether this is still the case with the advent of social media platforms, which potentially offer journalists other sources. Moreover, the integration of social media sources, such as Twitter or Telegram, is just one of many interdependencies in hybrid media environments. We aim to provide a more holistic understanding of sourcing practices in times of war by analyzing to what extent information from government and military sources, social media, other news media, and news agencies is featured as a main source in reporting on the war in Ukraine.

Methods

In our paper, we examine how 13 online media in Switzerland cover the war in Ukraine during the first 3 months after Russia's invasion—an example of a period in which journalists must typically identify reliable sources for reporting on the events surrounding such a war. Using a manual content analysis of 1,198 news articles, we analyze the sourcing practices that are visible in the reporting.

Results

Our results clearly show that information from other news outlets and social media and, above all, from news agencies plays an important role. Structural features of media types lead to distinct sourcing practices. Heavily commercialized, advertising-based media rely on news agency reports, other news media, and social media much more than subscription media or public service media. However, in all media types, actors from the government and the military are the most important source type.

Discussion

Our study reveals patterns old and new in terms of sourcing practices war coverage in a European country not participating in, but affected by, the major war in Ukraine.
Time-varying associations between loneliness and physical activity: Evidence from repeated daily life assessments in an adult lifespan sample
Authors: Broen Tiana , Choi Yoonseok , Zambrano Garza Elizabeth , Pauly Theresa , Gerstorf Denis , Hoppmann Christiane A.
Published: January 2023

Physical activity is a behavior that promotes physical and mental health; yet physical activity has decreased during the COVID-19 pandemic. To promote health during times of challenge, it is important to identify potential barriers to this key health behavior, such as loneliness. This brief report extends previous research on physical activity and loneliness that mainly focused on between-person differences to examine their time-varying associations at the within-person level using repeated daily life assessments. From April 2020 to August 2020, data were collected from a sample of 139 community-dwelling Canadian adults (Mage = 40.65 years, SD = 18.37; range = 18–83 years). Each evening for 10 consecutive days, participants reported their loneliness, number of steps, and minutes of moderate-to-vigorous physical activity. Results revealed that, in line with our hypotheses, on days when participants reported more loneliness they also engaged in less moderate-to-vigorous physical activity than on less lonely days (estimate = −0.24, p = 0.007); there was a significant negative association between loneliness and daily number of steps (estimate = −18.42, p = 0.041). In contrast, at the between-person level, overall loneliness was not associated with overall physical activity engagement after accounting for within-person differences and control variables (age, sex, day in study). From an intervention perspective, our findings suggest that it is promising to tackle loneliness on a day-to-day basis to increase physical activity one day at a time. This may be especially relevant during times mandating social-distancing, but also at other times when individuals experience greater feelings of loneliness.
The problem-solving method: Efficacy for learning and motivation in the field of physical education
Authors: Ezeddine Ghaith , Souissi Nafaa , Masmoudi Liwa , Trabelsi Khaled , Puce Luca , Clark Cain C. T. , Bragazzi Nicola Luigi , Mrayah Maher
Published: January 2023

Background

In pursuit of quality teaching and learning, teachers seek the best method to provide their students with a positive educational atmosphere and the most appropriate learning conditions.

Objectives

The purpose of this study is to compare the effects of the problem-solving method vs. the traditional method on motivation and learning during physical education courses.

Methods

Fifty-three students (Mage 15 ± 0.1 years), in their 1st year of the Tunisian secondary education system, voluntarily participated in this study, and randomly assigned to a control or experimental group. Participants in the control group were taught using the traditional methods, whereas participants in the experimental group were taught using the problem-solving method. Both groups took part in a 10-hour experiment over 5 weeks. To measure students' situational motivation, a questionnaire was used to evaluate intrinsic motivation, identified regulation, external regulation, and amotivation during the first (T0) and the last sessions (T2). Additionally, the degree of students' learning was determined via video analyses, recorded at T0, the fifth (T1), and T2.

Results

Motivational dimensions, including identified regulation and intrinsic motivation, were significantly greater (all p < 0.001) in the experimental vs. the control group. The students' motor engagement in learning situations, during which the learner, despite a degree of difficulty performs the motor activity with sufficient success, increased only in the experimental group (p < 0.001). The waiting time in the experimental group decreased significantly at T1 and T2 vs. T0 (all p < 0.001), with lower values recorded in the experimental vs. the control group at the three-time points (all p < 0.001).

Conclusions

The problem-solving method is an efficient strategy for motor skills and performance enhancement, as well as motivation development during physical education courses.
Work-family enrichment and successful aging at work: The China context
Authors: Zhao Chenhui , Ma Huajun , Chen Zimeng , Liu Xiaohui
Published: January 2023

Existing research mainly analyzes the antecedents of successful aging at work from the perspective of the work field, ignoring that in the Chinese context of “familism,” the two fields of family and work permeate each other and may have an impact on successful aging at work. Thus, through a multi-time data collection approach, we obtained a sample of 338 older Chinese employees to examine the impact of work-family enrichment on successful aging at work, the mediating role of occupational future time perspective, and the moderating role of age-inclusive human resource practice. Results indicate that work-to-family enrichment was positively associated with successful aging at work through the mediation of occupational future time perspective. Family-to-work enrichment was positively associated with successful aging at work through the mediation of occupational future time perspective. In addition, age-inclusive human resource practice amplified the positive effects of work-to-family enrichment and family-to-work enrichment on occupational future time perspective. This is an exploration of successful aging at work in the Chinese context, broadening the theoretical research on successful aging at work and providing new ideas for managers on motivating older employees to achieve successful aging at work.
Typicality and trajectories of problematic and positive behaviors over adolescence in eight countries
Authors: Buchanan Christy M. , Zietz Susannah , Lansford Jennifer E. , Skinner Ann T. , Di Giunta Laura , Dodge Kenneth A. , Gurdal Sevtap , Liu Qin , Long Qian , Oburu Paul , Pastorelli Concetta , Sorbring Emma , Steinberg Laurence , Tapanya Sombat , Uribe Tirado Liliana Maria , Yotanyamaneewong Saengduean , Alampay Liane P. , Al-Hassan Suha , Bacchini Dario , Bornstein Marc H. , Chang Lei , Deater-Deckard Kirby
Published: January 2023

In this study, we examine the predictions of a storm and stress characterization of adolescence concerning typicality and trajectories of internalizing, externalizing, and wellbeing from late childhood through late adolescence. Using data from the Parenting Across Cultures study, levels and trajectories of these characteristics were analyzed for 1,211 adolescents from 11 cultural groups across eight countries. Data were longitudinal, collected at seven timepoints from 8 to 17 years of age. Results provide more support for a storm and stress characterization with respect to the developmental trajectories of behavior and characteristics from childhood to adolescence or across the adolescent years than with respect to typicality of behavior. Overall, adolescents’ behavior was more positive than negative in all cultural groups across childhood and adolescence. There was cultural variability in both prevalence and trajectories of behavior. The data provide support for arguments that a more positive and nuanced characterization of adolescence is appropriate and important.
Happy and sad music acutely modulate different types of attention in older adults
Authors: Dovorany Nicholas , Brannick Schea , Johnson Nathan , Ratiu Ileana , LaCroix Arianna N.
Published: January 2023

Of the three subtypes of attention outlined by the attentional subsystems model, alerting (vigilance or arousal needed for task completion) and executive control (the ability to inhibit distracting information while completing a goal) are susceptible to age-related decline, while orienting remains relatively stable. Yet, few studies have investigated strategies that may acutely maintain or promote attention in typically aging older adults. Music listening may be one potential strategy for attentional maintenance as past research shows that listening to happy music characterized by a fast tempo and major mode increases cognitive task performance, likely by increasing cognitive arousal. The present study sought to investigate whether listening to happy music (fast tempo, major mode) impacts alerting, orienting, and executive control attention in 57 middle and older-aged adults (M = 61.09 years, SD = 7.16). Participants completed the Attention Network Test (ANT) before and after listening to music rated as happy or sad (slow tempo, minor mode), or no music (i.e., silence) for 10 min. Our results demonstrate that happy music increased alerting attention, particularly when relevant and irrelevant information conflicted within a trial. Contrary to what was predicted, sad music modulated executive control performance. Overall, our findings indicate that music written in the major mode with a fast tempo (happy) and minor mode with a slow tempo (sad) modulate different aspects of attention in the short-term.
Changes in executive function in the Canadian longitudinal study on aging over 3-years: A focus on social determinants of health
Authors: Stinchcombe Arne , Hammond Nicole G. , Hopper Shawna
Published: January 2023

Maintaining executive functions, including planning, inhibition, and decision-making skills, is important for autonomy and activities of daily living. There is a growing body of evidence linking social determinants and cognitive aging, but less is known about the potential role of social determinants in changes in executive functioning over time. Using data from the Canadian Longitudinal Study on Aging (CLSA), a large cohort of mid-aged and older adults, we examined changes in executive function over a 3-year period. Specifically, we focused on the role of social determinants (i.e., social positioning, social support, education) in explaining these changes. Executive function was measured at baseline and follow-up 3 years later using the Mental Alteration Test (MAT). We computed a reliable change index (RCI) and used a multiple linear regression model to examine the associations between known correlates and change in executive function over the 3-year period (n = 29,344). Older age, higher household income, and greater educational attainment predicted declines in executive function. Health factors (e.g., depression symptoms, physical activity levels) and many social determinants (sexual orientation, gender identity, race, and perceived social standing) were not associated with change in executive function. These results suggest that social determinants of health may be related to initial differences in cognitive functioning (i.e., cross-sectional differences) rather than more rapid cognitive aging.
Attitudes of medical students toward psychiatry in Eastern Mediterranean Region: A systematic review
Authors: Mohebbi Mohammad , Nafissi Nastaran , Ghotbani Farzaneh , Khojasteh Zonoozi Arash , Mohaddes Ardabili Hossein
Published: January 2023

Background

Psychiatry is facing one of the highest levels of shortages among medical specialties. Stigma toward psychiatry plays an influential role in medical students' decision to choose psychiatry as a career and has been reported to be prevalent in different parts of the world, particularly in low/middle-income countries.

Objective

To systematically review the Eastern Mediterranean Region (EMR) medical students' attitudes toward psychiatry, to assess whether their attitudes are stigmatized or not, and the factors affecting their attitudes.

Method

PubMed, Scopus, Web of Science, and PsychInfo (PsycARTICLES) were searched using a combination of main terms “stigma,” “psychiatry,” “medical students,” and the name of Eastern Mediterranean countries. Cross-sectional studies assessing the attitudes of EMR medical students toward psychiatry were included in this review.

Results

Ten studies were eligible to be included in the result synthesis. These were from Pakistan (n = 3), Iran (n = 2), Saudi Arabia (n = 1), Lebanon (n = 1), Egypt (n = 1), Bahrain (n = 1), and Oman (n = 1). Most studies reported a combination of both positive and negative attitudes among medical students; however, the overall attitude was positive. Factors affecting medical students' attitude toward psychiatry included poor psychological well-being, having a friend with a psychiatric illness, involving in a romantic relationship with someone suffering from mental illness, young age, frequency of exposure to psychiatry clerkship/teaching, and quality of psychiatry clerkship. Nevertheless, the final positive or negative outcome of these factors on students' attitudes remained controversial.

Conclusion

Considering the lack of sufficient data from most EMR countries, we need to exercise caution in interpreting the results of this review. Nevertheless, our review indicates that psychiatry is not stigmatized among EMR medical students, and they have generally positive attitudes toward it. The findings of studies evaluating influencing factors are contradictory and demand further exploration.
“We just have to help”: Community health workers' informal task-shifting and task-sharing practices for hypertension and diabetes care in Nigeria
Authors: Ajisegiri Whenayon Simeon , Abimbola Seye , Tesema Azeb Gebresilassie , Odusanya Olumuyiwa O. , Peiris David , Joshi Rohina
Published: January 2023

Introduction

Nigeria's skilled health professional health workforce density is insufficient to achieve its national targets for non-communicable diseases (NCD) which include 25% reduction in the prevalence of diabetes and hypertension, particularly at the primary health care (PHC) level. This places a great demand on community health workers (CHWs) who constitute the majority of PHC workers. Traditionally, CHWs are mainly involved in infectious diseases programmes, and maternal and child health services. Their involvement with prevention and control of NCDs has been minimal. With government prioritization of PHC for combating the rising NCD burden, strengthening CHWs' skills and competencies for NCD care delivery is crucial.

Methods

We conducted a mixed methods study to explore the roles and practices of CHWs in the delivery of hypertension and diabetes care at PHC facilities in four states (two each in northern and southern regions) in Nigeria. We reviewed the National Standing Orders that guide CHWs' practices at the PHC facilities and administered a survey to 76 CHWs and conducted 13 focus groups (90 participants), and in-depth individual interviews with 13 CHWs and 7 other local and state government stakeholders.

Results

Overall, we found that despite capacity constraints, CHWs frequently delivered services beyond the scope of practice stipulated in the National Standing Orders. Such informal task-shifting practices were primarily motivated by a need to serve the community.

Discussion

While these practices may partially support health system functions and address unmet need, they may also lead to variable care quality and safety. Several factors could mitigate these adverse impacts and strengthen CHW roles in the health system. These include a stronger enabling policy environment to support NCD task-sharing, investment in continuous capacity building for CHWs, improved guidelines that can be implemented at the point of care, and improved coordination processes between PHC and higher-level facilities.
Relationship between meteorological and environmental factors and acute exacerbation for pediatric bronchial asthma: Comparative study before and after COVID-19 in Suzhou
Authors: Guo Suyu , Chen Dongmei , Chen Jiawei , Zhu Canhong , Huang Li , Chen Zhengrong
Published: January 2023

Objective

Climate and environmental change is a well-known factor causing bronchial asthma in children. After the outbreak of coronavirus disease (COVID-19), climate and environmental changes have occurred. The present study investigated the relationship between climate changes (meteorological and environmental factors) and the number of hospitalizations for pediatric bronchial asthma in Suzhou before and after the COVID-19 pandemic.

Methods

From 2017 to 2021, data on daily inpatients diagnosed with bronchial asthma at Children's Hospital of Soochow University were collected. Suzhou Meteorological and Environmental Protection Bureau provided daily meteorological and environmental data. To assess the relationship between bronchial asthma-related hospitalizations and meteorological and environmental factors, partial correlation and multiple stepwise regression analyses were used. To estimate the effects of meteorological and environmental variables on the development of bronchial asthma in children, the autoregressive integrated moving average (ARIMA) model was used.

Results

After the COVID-19 outbreak, both the rate of acute exacerbation of bronchial asthma and the infection rate of pathogenic respiratory syncytial virus decreased, whereas the proportion of school-aged children and the infection rate of human rhinovirus increased. After the pandemic, the incidence of an acute asthma attack was negatively correlated with monthly mean temperature and positively correlated with PM2.5. Stepwise regression analysis showed that monthly mean temperature and O3 were independent covariates (risk factors) for the rate of acute asthma exacerbations. The ARIMA (1, 0, 0) (0, 0, 0) 12 model can be used to predict temperature changes associated with bronchial asthma.

Conclusion

Meteorological and environmental factors are related to bronchial asthma development in children. The influence of meteorological and environmental factors on bronchial asthma may be helpful in predicting the incidence and attack rates.
An intelligent medical guidance and recommendation model driven by patient-physician communication data
Authors: Liu Jusheng , Li Chaoran , Huang Ye , Han Jingti
Published: January 2023

Based on the online patient-physician communication data, this study used natural language processing and machine learning algorithm to construct a medical intelligent guidance and recommendation model. First, based on 16,935 patient main complaint data of nine diseases, this study used the word2vec, long-term and short-term memory neural networks, and other machine learning algorithms to construct intelligent department guidance and recommendation model. Besides, taking ophthalmology as an example, it also used the word2vec, TF-IDF, and cosine similarity algorithm to construct an intelligent physician recommendation model. Furthermore, to recommend physicians with better service quality, this study introduced the information amount of physicians' feedback to the recommendation evaluation indicator as the text and voice service quality. The results show that the department guidance model constructed by long-term and short-term memory neural networks has the best effect. The precision is 82.84%, and the F1-score is 82.61% in the test set. The prediction effect of the LSTM model is better than TextCNN, random forest, K-nearest neighbor, and support vector machine algorithms. In the intelligent physician recommendation model, under certain parameter settings, the recommendation effect of the hybrid recommendation model based on similar patients and similar physicians has certain advantages over the model of similar patients and similar physicians.
Spatial effects of environmental regulation on high-quality economic development: From the perspective of industrial upgrading
Authors: Li Yangyang , Liu Weijiang
Published: January 2023

Studying the spatiotemporal heterogeneity of environmental regulations on high-quality regional economic development is of enormous practical value in the context of sustainable economic, social, and environmental development. Only a few studies, however, examined the regional heterogeneity of environmental regulation affecting economic development from the standpoint of upgrading the industrial structure. This research investigated the spatial distribution traits of high-quality regional development based on the construction of a comprehensive assessment index system for high-quality economic development. The economic geography-nested spatial Durbin model is then used to perform an empirical test. The findings demonstrate that (1) high-quality economic development has visible spatial heterogeneity, with strong local spatial agglomeration between regions; (2) environmental regulation and the modernization of the industrial structure are significant variables influencing high-quality economic development, but their development is not balanced; and (3) environmental policies promote high-quality regional development through a distinct channel. Formal environmental regulation promotes economic development through rationalizing industrial structure, while informal environmental regulation does so through upgrading the industrial structure. Further, both kinds of environmental regulation have positive spatial spillover effects on adjacent areas. Therefore, the regional heterogeneity of environmental regulation and industrial structure is of great significance in promoting the high-quality and sustainable development of regional economies.
Internet use and rural-urban mental health inequalities: Evidence from China
Authors: Nie Weihao , Hu Mingzheng , Ye Xin
Published: January 2023

Introduction

In the context of the new digital era, clarifying the relationship between Internet use and urban and rural residents' mental health is of important value for reducing rural-urban health inequalities. This paper aims to study the association between Internet use and rural-urban mental health inequalities.

Methods

Based on the data of the China Family Panel Studies (CFPS) in 2020, we firstly examined the existence and specific manifestation of mental health inequalities between urban and rural residents. Secondly, we examined the mediating effect of Internet use by the Bootstrap mediating effect measure. Finally, we verified the robustness of the mediating effect.

Results

There are significant mental health inequalities between urban and rural residents, and urban residents have better mental health than rural residents (p < 0.01). In addition, the test results for the mediating effect of Internet use on mental health inequalities between urban and rural residents were significant (p < 0.01), with a direct effect of −0.028 (p < 0.01) and an indirect effect of −0.49 (p < 0.01), and this result remained significant in the robustness test.

Discussion

In such a new age of the Internet, mental health inequalities between urban and rural residents objectively did exist, and the use of the internet played a positive mediation effect on the formation of mental health inequalities between urban and rural areas.
Social configurations in the moment of post-foundationalism
Authors: Jong Abbas
Published: January 2023

Modern social sciences arose during a period of classical modernity in which discovering universal rules between distinct phenomena was the most prominent criterion of scientific knowledge. Social phenomena were considered in the form of isolated, determined, standardized, and regulated objects whose knowledge, like that of the natural sciences, depended on the understanding of universal laws. The accidental and the contingent were eliminated in favor of universal laws. With the intensifying of modernity and the transition to late and liquid modernity, and by suspending many dominant cognitive categories, this kind of essentialist foundationalism was attacked by a variety of anti/non-foundationalist criticism that subscribed to either plural grounds or groundlessness, a bottomless ground in which scientific knowledge at a high level lost its significance. This predicament has given rise to several biases and antinomies in modern social theory. By addressing some of these predicaments and antinomies, including foundationalism/non(anti-)foundationalism, agency/structure, the individual/society, essentialism/relativism, and universalism/singularism, the present article strives to propose the idea of social configurations as a solution to overcome them, and through this endeavor, it is indicated that considering these configurations can effectively explain emerging and interrelated global phenomena. By prioritizing the conditions of possibility for social phenomena, and taking into account their contingency, as well as the incompleteness and partiality of their foundations, social configurations are considered as units at the level of the particular whose relationality, indeterminacy, interdependence, and fluidity constitute their central features.
Taboos, food avoidances, and diseases: Local epistemologies of health among Coastal Endenese in Eastern Indonesia
Authors: Ramenzoni Victoria Constanza
Published: January 2023

To advance interventions targeting malnutrition among small-scale fishing societies, knowledge on the role played by taboos and dietary avoidances on the consumption of fish related products becomes crucial. The article builds upon ethnographic fieldwork (participant observation, focus groups and interviews), dietary questionnaires (n: 112), and archival research among Coastal Endenese in Eastern Indonesia to understand the role played by taboos and dietary preferences in regulating intake of marine products. Moving beyond binary notions of “good” and “bad” when considering the food-system implications of taboos, it explores how local beliefs about illness and food shape dietary practices that can have concrete consequences for an individual's health. Endenese consider fish as having originated from terrestrial creatures, creating a continuous cycle of movement from land to sea and back. Within this cosmology, food is seen as a medium that can bring about healthy outcomes but also disease. Results emphasize the need to understand taboos and food avoidances within the larger cosmological and religious system but also underscore the changing nature of dietary preferences and values due to market integration processes which may have long-term repercussions on health. This information is key to the design of culturally sensitive dietary strategies and alternative livelihoods approaches that seek to minimize poverty.
Replacing alfalfa hay with industrial hemp ethanol extraction byproduct and Chinese wildrye hay: Effects on lactation performance, plasma metabolites, and bacterial communities in Holstein cows
Authors: Wang Yiqiang , Yu Qingyuan , Wang Xiaolin , Song Jiamei , Lambo Modinat Tolani , Huang Jianguo , He Ping , Li Yang , Zhang Yonggen
Published: January 2023

This trial was designed to investigate the effects of industrial hemp ethanol extraction byproduct (IHEEB) and Chinese wildrye hay (CWH) replacement of alfalfa hay (AH) on digestibility, and lactation performance, plasma metabolites, ruminal fermentation, and bacterial communities in Holstein dairy cows. Nine healthy multiparous Holstein cows (parity = 3) with similar body weights (584 ± 12.3 kg), days in milk (108 ± 11.4), and milk yields (30 ± 1.93 kg; all mean ± standard deviation) were used in a replicated 3 × 3 Latin square design with 3 periods of 21 d. During each period, each group consumed 1 of 3 diets: (1) 0% IHEEB (0IHEEB); (2) 6.0% IHEEB and 1.7% Chinese wildrye hay (6IHEEB); (3) 10.8% IHEEB and 4.3% Chinese wildrye hay (11IHEEB). The diets in each group were isocaloric and isonitrogenous, with similar contents of concentrate and silage but different ratios of IHEEB and CWH to replace AH. The results showed that increasing the substitute did not affect the total-tract apparent nutrient digestibility. There was no difference in lactation performance of dairy cows fed the three diets, except for the cows' somatic cell count (SCC), which decreased with the increase in the amount of the substitute. Cannabidiol and tetrahydrocannabinol were not detected in milk samples of dairy cows in the different treatment groups. 6IHEEB and 11IHEEB-fed cows showed a linear decrease in total volatile fatty acids (VFA) and butyrate compared to the 0IHEEB cows. Plasma IL-1β content quadratically decreased with feeding IHEEB and CWH, and other blood parameters were unaffected. The rumen fluid's relative abundances of Bacteroidota, Fibrobacterota, and Prevotellaceae quadratically increased, while Firmicutes tended to decrease quadratically as the substitution increased. Feeding IHEEB and CWH linearly increased the relative abundances of Firmicutes, Lachnospiraceae, Monoglobaceae, and Butyricicoccaceae in the feces. As the substitution increased, the cost of dairy farming was reduced. In summary, substituting AH with IHEEB and CWH in diets did not affect the total-tract apparent nutrient digestibility, improved milk composition, and plasma immune indices. It changed the bacterial composition in rumen fluid and feces and improved dairy farming benefits.
Growth performance, jejunal morphology, disaccharidase activities, and sugar transporter gene expression in Langde geese as affected by the in ovo injection of maltose plus sucrose
Authors: Li Desheng , Dang De Xin , Xu Han , Zhou Haizhu , Lou Yujie , Liu Xiao , Cui Yan
Published: January 2023

Introduction

The vigorous metabolic activity of an embryo increases the risk of low energy supply during incubation. The lack of energy during this critical period will lead to the death of an embryo. To avoid this risk, the in ovo injection technique in ovo allows for the injection of energy substances into an embryo.

Methods

This study investigated the effects of in ovo injection of maltose and sucrose (MS) in ovo on post-hatching growth performance, jejunal morphology and disaccharidase activities, and sugar transporter gene expression in Langde geese. A total of 300 fertilized eggs (115.75 ± 1.25 g) obtained from 3-year-old Langde geese were used in this study. The eggs were randomly assigned to two groups, and the difference between the two groups was whether 25g/L maltose and 25g/L sucrose (MS) dissolved in 7.5g/L NaCl were injected into the amnion on embryonic day 24. Each group had six replicates, which each replicate containing 25 eggs. The goslings were raised till day 28.

Results and discussion

The results showed that the in ovo injection of MS increased final body weight, average daily gain (ADG), and feed efficiency. Additionally, MS injection improved post-hatching jejunal morphology, disaccharidase activities, and sugar transporter gene expression at an early stage. Therefore, we considered that the in ovo injection of MS had positive effects on the nutrient absorption capacity of goslings, thus contributing to the improvement in their growth performance.
Induction of autophagy improves skin and hair conditions in dogs with underlying diseases
Authors: Kim Yoonji , Lee Seung-Hwa , Song Yunji , Jeong Sekyoo , Kim Ha-Jung
Published: January 2023

Background

Autophagy was reported to play a crucial role in maintaining general and skin health.

Methods

The study used a synthesized autophagy inducer (AI) (Aquatide™ cospharm Inc.; Daejeon, Korea), for evaluating the effects of autophagy on skin and hair in dogs. Twenty-two dogs with poor skin and hair which were diagnosed with canine atopic dermatitis (CAD) or pituitary-dependent hyperadrenocorticism (PDH) were included. Clinical scores using Canine Atopic Dermatitis Extent and Severity Index-04 (CADESI-04), Pruritus Visual Analog Scale (PVAS) and skin barrier function using measurement of transepidermal water loss (TEWL) were evaluated and canine keratinocytes were also used in vitro investigation of pro-inflammatory cytokines after AI treatment.

Results

In the AI group, clinical scores and skin barrier function were improved at week 8 significantly compared to in the other groups. In particular, the AI significantly improved the hair surface damage at 8 weeks compared to the baseline. In vitro, the AI reduced pro-inflammatory cytokines by activating the 78-kDa glucose-regulated protein (GRP78).

Conclusion

AI improve skin barrier function and hair damage and reduce pro-inflammatory cytokines by inhibiting reactive oxygen species (ROS) production in dogs.
Evaluation of Platelet-Rich Plasma by means of PRGF®-Endoret® protocol in leukemia cats: PDGF-BB and TGF-ß1 valuation
Authors: Miguel-Pastor Laura , Satué Katy , Chicharro Deborah , Peláez Pau , Torres-Torrillas Marta , Carrillo José M. , Cerón José J. , Sopena Joaquín J. , Rubio Mónica
Published: January 2023

Introduction

Feline leukemia virus (FeLV) is a chronic disease that leads to the weakening of a cat's immune system. Platelet-rich plasma (PRP) offers therapeutic effects for multiple diseases, the use of PRP and growth factors (GFs) determination could be an alternative treatment to improve the quality of life in these patients. The objectives of this study were to determine and compare the concentration of platelets (PLTs), red blood cells (RBCs) and white blood cells (WBCs) between samples of whole blood (WB), PRP and platelet-poor plasma (PPP) fractions, and to evaluate the concentration of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in both fractions in FeLV cats using a PRGF®-Endoret® protocol previously standardized in this species.

Methods

WB was collected from 11 asymptomatic FeLV-positive cats. PRP and PPP was obtained following PRGF®-Endoret® technology according to centrifugation at 265 g for 10 min. Cellular components, RBCs, WBCs, PLTs, and the PDGF-BB and TGF-β1 concentrations in PRP and PPP fractions were determined.

Results

PLT in the PRP fraction was statistically higher than WB and PPP fraction, with no statistical differences between WB and PPP. PLT concentration increased 1.4 times in PRP fraction compared to WB. Mean platelet volume (MPV) did not differ significantly between the WB, PRP, and PPP fractions. Compared to WB, the absolute numbers of RBCs and WBCs were decreased by 99% and more than 95% in the PRP and PPP fractions, respectively. TGF-ß1 concentrations increased in PRP vs. PPP, with no changes in PDGF-BB.

Discussion

Based on the degree of PLT enrichment and the absence of RBCs and WBCs, this blood product could be classified as a Pure Platelet-Rich Plasma (P-PRP). The presence of GFs in PRP and PPP samples suggests that the PRGF®-Endoret® methodology is suitable for obtaining PRP in FeLV cats, despite future studies are necessary to optimize the technique, standardize the results and assess clinical efficacy.
Molecular Genetic Mechanisms of Heterosis in Sugarcane Cultivars Using a Comparative Transcriptome Analysis of Hybrids and Ancestral Parents
Authors: Feng Mengfan , Zhao Jihan , Li Sicheng , Wei Ni , Kuang Bowen , Yang Xiping
Published: January 2023

Modern sugarcane cultivars (Saccharum spp. hybrids) are the major contributors to sucrose and bioenergy in the world. The global changes in gene expression and the molecular mechanism of heterosis between modern sugarcane hybrids and their parents remain to be elucidated. In this study, we performed a comparative transcriptome analysis between hybrids and their parents using the Illumina RNA-Seq method to understand the differences in transcript expression after hybridization. The results show that (1) introduction of the S. spontaneum lineage resulted in significant upregulation of biotic and abiotic stress resistance genes in S. hybrids, including hexokinase (HXK) genes, pathogenesis-related protein (PR1) genes, coronatine-insensitive protein (COI-1), jasmonate ZIM domain-containing protein (JAZ) genes, and serine/threonine protein kinase 2 (SnRK2) genes. (2) Transgressive genes in hybrids were mainly concentrated in the synthesis pathways of biotin and vitamin B6, helping establish advantages in terms of stress resistance, antioxidant activity, and growth. (3) Glutathione-S-transferase (GST) was likely to enhance stress resistance in hybrids, and corresponding genes were key positive selection genes in processes, including round-robin selection and other adaptations. In this study, we propose explanations for heterosis in sugarcane hybrids from a transcriptomic perspective, in addition to identifying candidate genes to aid in the improvement of sugarcane cultivars.
Donkey Heart Rate and Heart Rate Variability: A Scoping Review
Authors: De Santis Marta , Seganfreddo Samanta , Greco Alberto , Normando Simona , Benedetti Daniele , Mutinelli Franco , Contalbrigo Laura
Published: January 2023

Heart rate (HR) and heart rate variability (HRV) are commonly used physiological measures in animals. While several studies exist on horse HRV, less information is available for donkeys. This scoping review aims to understand the extent and type of published evidence on donkey HR and HRV, their clinical and research applications, the devices used, and the analysis performed. Only quantitative primary studies published in English were considered. Four different databases were queried through the Web of Science platform, with additional evidence identified by citation chasing. After a two-stage screening phase, data were extracted considering study and population characteristics, information on HR/HRV analysis, and applications. The majority of the 87 included articles (about 80%) concerned a sample size of up to 20 individuals and were published since 2011 (about 65%). Forty-one articles employed an electronic device for signal acquisition (mainly electrocardiographs and heart rate monitors), yet only two articles reported HRV parameters. The literature on donkey HRV is lacking, and this gap can be filled by gaining knowledge on donkey characteristics and finding useful tools for welfare assessment. Comparison with what is known about the horse allows a discussion of the technical and interpretative difficulties that can be encountered with donkeys.
Design and Mechanical Properties Analysis of Variable Buffer-Force Planing Energy-Absorbing Device for Rail Vehicles
Authors: Wei Wei , Zhang Fengqiang , Xing Youdong , Wang Hongxiang , Liu Rongqiang
Published: January 2023

Collisions between rail transit vehicles are unavoidable. In order to alleviate the disaster caused by the collision, energy-absorbing and shock-absorbing materials are generally installed at the front end of the rail vehicle. In this paper, a variable buffer-force planing energy-absorbing device for rail transit vehicles was prepared. The buffer force was changed by length (Dx), angle (A) and thickness (W). First, we manufactured one type of material, and data were obtained through experimentation. Second, we used Ls-DYNA to simulate this material, and determined the accuracy between the simulation and the test. Third, various parameters of the material were simulated by Ls-DYNA. The results show that changing Dx greatly affects the performance of the material, W has no obvious effect on the performance, and A is important for the materials properties. Based on finite element simulation, the functional model relationship between the average force and various parameters was deduced. This lays the groundwork for the application of the variable buffer-force planing energy absorbing device.
Physical Model of D-Region Ionosphere and Preliminary Comparison with IRI and Data of MF Radar at Kunming
Authors: Zhu Mengyan , Xu Tong , Sun Shuji , Zhou Chen , Hu Yanli , Ge Shucan , Li Na , Deng Zhongxin , Zhang Yuqiang , Liu Xiaolei
Published: January 2023

Based on the ion continuity equation solved under chemical equilibrium, a physical model of the D-region ionosphere (60–90 km) is established. The model involves 145 ion chemical reactions and includes 23 kinds of positive ions, 11 kinds of negative ions, and electrons. The simulation results show that molecular ions, such as NO+, NO+(H2O)n, H+(H2O)n, CO3−, and O3−, are the main components of ions in the D-region. The diurnal change of electron density at low latitudes is more obvious than at high latitudes. Preliminary comparisons with the International Reference Ionosphere (IRI) model and observed data of Medium Frequency (MF) radar at Kunming Radio Wave Observation Station show that the model is able to describe the basic features of D-region parameters. In addition, the results show that the minimum height of the D-region lower boundary in the low latitude is approximately 65 ± 1 km, and the height during the daytime is strongly correlated with local time. Furthermore, the results also reveal that the asymmetry of electron density is observed, with higher electron density during sunset than during sunrise at 75–85 km altitude. These above results are helpful for better understanding the variation of the D-region.
A Comparative Study of Deep Learning Models on Tropospheric Ozone Forecasting Using Feature Engineering Approach
Authors: Rezaei Reza , Naderalvojoud Behzad , Güllü Gülen
Published: January 2023

This paper investigates the effect of the architectural design of deep learning models in combination with a feature engineering approach considering the temporal variation in the features in the case of tropospheric ozone forecasting. Although deep neural network models have shown successful results by extracting features automatically from raw data, their performance in the domain of air quality forecasting is influenced by different feature analysis approaches and model architectures. This paper proposes a simple but effective analysis of tropospheric ozone time series data that can reveal temporal phases of the ozone evolution process and assist neural network models to reflect these temporal variations. We demonstrate that addressing the ozone evolution phases when developing the model architecture improves the performance of deep neural network models. As a result, we evaluated our approach on the CNN model and showed that not only does it improve the performance of the CNN model, but also that the CNN model in combination with our approach boosts the performance of the other deep neural network models such as LSTM. The development of the CNN, LSTM-CNN, and CNN-LSTM models using the proposed approach improved the prediction performance of the models by 3.58%, 1.68%, and 3.37%, respectively.
A Review of Conventional and Novel Treatments for Osteoporotic Hip Replacements
Authors: Alabdah Fahad , Alshammari Adel , Hidalgo-Bastida Araida , Cooper Glen
Published: January 2023

Introduction: Osteoporosis is a skeletal disease that severely affects the mechanical properties of bone. It increases the porosity of cancellous bone and reduces the resistance to fractures. It has been reported in 2009 that there are approximately 500 million osteoporotic patients worldwide. Patients who suffer fractures due to fragility cost the National Healthcare Systems in the United Kingdom £4.4 billion in 2018, in Europe €56 billion in 2019, and in the United States $57 billion in 2018. Thus, osteoporosis is problematic for both patients and healthcare systems. Aim: This review is conducted for the purpose of presenting and discussing all articles introducing or investigating treatment solutions for osteoporotic patients undergoing total hip replacement. Methods: Searches were implemented using three databases, namely Scopus, PubMed, and Web of Science to extract all relevant articles. Predetermined eligibility criteria were used to exclude articles out of the scope of the study. Results: 29 articles out of 183 articles were included in this review. These articles were organised into three sections: (i) biomechanical properties and structure of osteoporotic bones, (ii) hip implant optimisations, and (iii) drug, cells, and bio-activators delivery through hydrogels. Discussion: The findings of this review suggest that diagnostic tools and measurements are crucial for understanding the characteristics of osteoporosis in general and for setting patient-specific treatment plans. It was also found that attempts to overcome complications associated with osteoporosis included design optimisation of the hip implant; however, only short-term success was reported, while the long-term stability of implants was compromised by the progressive nature of osteoporosis. Finally, it was also found that targeting implantation sites with cells, drugs, and growth factors has been outworked using hydrogels, where promising results have been reported regarding enhanced osteointegration and inhibited bacterial and osteoclastic activities. Conclusions: These results may encourage investigations that explore the effects of these impregnated hydrogels on osteoporotic bones beyond metallic scaffolds and implants.