bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations Between Symptoms and Quality of Life in Lumbar Degenerative Disease and Cognitive Dysfunction in a Japanese Community: A Cross-sectional Study
Authors: Koyama Kazushige , Wada Kanichiro , Kumagai Gentaro , Kudo Hitoshi , Tanaka Sunao , Asari Toru , Ihara Kazunari , Nakaji Shigeyuki , Ishibashi Yasuyuki
Published: August 2020

Background: Lumbar degenerative disease (LDD) and dementia are increasing in super-aging societies and are both related to physical dysfunction and pain. However, the relationship between these diseases remains unclear. This cross-sectional study aimed to investigate the rate of comorbidity of lumbar spinal stenosis (LSS) and mild cognitive impairment (MCI) by age and sex and clarify the association between LSS presence, LDD symptoms, quality of life (QOL) related to low back pain, and cognitive impairment in a Japanese population.Methods: We enrolled 1097 participants (men 437; women 660; mean age 54.6 years) from a medical checkup program. LSS was diagnosed using a self-administered questionnaire, and LDD symptoms were evaluated using visual analog scale (VAS: low back pain, pain, numbness of the lower limb). QOL related to low back pain was evaluated using the Japanese Orthopedic Association Back-Pain Evaluation Questionnaire (JOABPEQ: pain, lumbar function, and gait function), and Kellgren-Lawrence grading was performed to evaluate lumbar degeneration using lateral radiographs of the lumbar spine. Cognitive function was measured using the Mini Mental State Examination (MMSE), and we defined MCI as a summary score of MMSE £27. Logistic and multiple liner regression analyses were performed to analyze the association between MCI, summary score of MMSE and LSS factors.Results: The comorbidity rate of MCI and LSS was 0.9% in men and 0.7% in women. It was 2.1% in those aged ³65 years in both sexes, and this rate increased with age. Lumbar function in JOABPEQ (OR 0.979, 95% CI 0.961 to 0.998) and lower limb pain in VAS (OR 1.020, 95% CI 1.002 to 1.039) were associated with MCI in men. The presence of LSS, lumbar function, and gait function in JOABPEQ were associated with MMSE in both sexes, while lower limb numbness and pain in VAS were associated with MMSE in men.Conclusion: The comorbidity rate of LSS and MCI increased with age. The presence of LSS, certain symptoms of LDD, and deterioration of QOL due to low back pain were related to cognitive dysfunction. Treatment of LDD could help to improve both LDS symptoms and cognitive dysfunction.
Association of XRCC1 rs1799782 and ERCC2 rs13181 Polymorphisms with Glioma Risk: A Systematic Review and Meta-Analysis
Authors: Tavares Cléciton Braga , Alves-Ribeiro Francisco Adelton , Junior Elmo de Jesus Nery , Vasconcelos-Valença Rodrigo Jose de , Campos-Verdes Larysse Cardoso , Gomes-Braga Francisca das Chagas Sheyla Almeida , Lopes-Costa Pedro Vitor , Santos Alesse Ribeiro dos , Pinho-Sobral André Luiz , Campelo Viriato , Cardoso Arquimedes Cavalcante , Sousa Emerson Brandao , Pereira Renato de Oliveira , Gebrim Luiz Henrique , Silva Benedito Borges da
Published: August 2020

BackgroundGliomas are the most common primary tumors of the central nervous system with unclear etiology, however hereditary factors may play important roles in the development of gliomas with mutations and single nucleotide polymorphisms (SNPs) being prominent among the genetic changes. this systematic review and meta-analysis assess the association of XRCC1 (rs1799782) and ERCC2 (rs13181) gene polymorphisms and glioma risk.MethodsThis study included articles indexed in the PUBMED and EMBASE databases published during the past 15 years. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed. The PICOS model was used to develop the inclusion criteria and search terms. This review was recorded at PROSPERO International prospective register of systematic reviews, ID 196173. The META-MAR V2.7.0 meta-analysis calculator was used for the statistical analysis with p-values < 0.05 being considered statistically significant. Dichotomous data are presented as odds ratios (OR) with a 95% confidence interval (CI). Statistical heterogeneity was measured using the I2 test and I2 > 50% were regarded as high heterogeneity. Funnel plots, Begg (BT) and Egger Tests (ET) were used to assess publication bias (p<0.1). Results Literature review identified 10 articles on ERCC2 gene rs13181 variant and 11 on XRCC1 rs1799782. The meta-analysis identified a risk for gliomas for the TT genotype of the XRCC1 rs1799782 SNP in Asians (OR: 1.59, 95% CI: 1.3-1.93; p=0.006; I2 13.1%). ERCC2 rs13181 polymorphisms identified as risks for gliomas were AC genotypes in Asians (OR: 2.06, 95% CI: 1.75-1.42; p = 0.00057; I2 91.1%) and Caucasians (OR: 1.16, 95% CI: 1.01-1.31; p = 0.02; I2 12.2%), and CC genotypes in Caucasians (OR: 2.06, 95% CI: 1.75-1.42; p = 0.0001; I2 98.9%).Conclusions TT genotypes of the XRCC1 rs1799782 SNP in Asians, AC genotypes of ERCC2 rs13181 polymorphisms in Asians and Caucasians, and CC genotypes of ERCC2 rs13181 polymorphisms in Caucasians were associated with increased risk for gliomas that may benefit these patients with early diagnostic and therapeutic strategies.
Plasma D-Dimer Level is Associated with Clinical Outcomes in Patients with Atrial Fibrillation Related Acute Ischemic Stroke after Pneumonia
Authors: Yang Xu , Lu Taoli , Qu Zhanli , Zhang Yi , Liu Pingping , Ma Ying
Published: August 2020

Objectives: Pneumonia is related to poor prognosis in acute ischemic stroke (AIS), and its risk might be higher in atrial fibrillation (AF) related AIS with elevated plasma D-dimer. The aim of our study was to investigate the prognostic value of D-dimer for predicting clinical outcome of AF-related AIS with pneumonia. Method: AF-related AIS patients with pneumonia were prospectively enrolled. Receiver operating characteristic (ROC) curve was used to determine the optimal D-dimer point for 3-month mortality and death/severe disability. The associations between the D-dimer and 3-month mortality and death/severe disability were assessed by multivariable logistic regression analysis. Results: A total of 415 patients were enrolled in this study. ROC curve analysis showed that the optimal cut point of D-dimer for 3-month death/severe disability and mortality were D-dimer≥2.35mg/l and D-dimer≥3.35mg/l, respectively. Multivariable logistic regression analysis showed that D-dimer≥2.35mg/l(OR 5.95, 95% CI: 3.09-11.45, P＜0.001), higher NIHISS score(OR:1.53, 95% CI: 1.38–1.68, P＜0.001) were associated with increased risk of 3-month death/severe disability), and anticoagulant was associated with decreased risk of death/severe disability (OR:0.16, 95% CI: 0.07-0.35, P＜0.001). Higher NIHISS score(OR:1.56, 95% CI: 1. .37–1.77, P＜0.001), higher age(OR 1.06, 95% CI: 1.01–1.11, P=0.019), D-dimer≥3.35mg/l (OR 7.85, 95% CI: 3.21-19.21, P=0.001)were associated with increased risk of 3-month mortality.Conclusions: AF-related AIS patients with concurrent high D-dimer and pneumonia increased risk of 3-month mortality and death/severe disability, plasma D-dimer may have predictive value in outcome after AF-related AIS with pneumonia.
Profile of parasuicide cases attending Brits Hospital, North West Province, South Africa: a 5-year chart review
Authors: Bongongo Tombo , Nkuinika K , Nzaumvila DK , Adfolalu A , Okonta H , Govender I , Saidiya CNS
Published: August 2020

Background Parasuicide is referred to as an apparent attempt at suicide without the actual intention of killing oneself. Its prevalence continues to increase in the Brits/Madibeng community. The current study aimed to determine the profile of parasuicide cases attending Brits Hospital, North West Province of South Africa, from 2013 to 2018. Methods The basis of this article is a 5-year retrospective chart review of parasuicide cases attending Brits Hospital from 01 January 2013 to 01 January 2018. Brits Hospital is a 200-bed hospital serving people in the Brits/Madibeng community. Results Of the 477 parasuicide cases, the mean age was 26.6, the median 24 years with minimum and maximum ages respectively of 12 and 64 years. The majority (257; 53.88%) were between 16 and 25 years of age and comprised females (297; 62.26%), African (391; 82.84%), single people (352; 73.79%), unemployed (304; 63.7%) and those who had completed the secondary level of education, 300 (62.9%). The highest number of cases (85, 86 and 87) were registered during the odd years (2013, 2015 and 2017), while the lowest number (68, 75 and 76) during even years, i.e. 2014, 2016 and 2017. The summer months of January and February registered more cases, respectively 65 (13.63%) and 64 (13.42%). The majority of cases (430; 90.15%) represented a first attempt at suicide. Overdose of medication (300; 62.82%) was the most common method used and a relationship problem the main reason that led to the attempt. Most cases (409; 85.74%) did not have any medical or psychiatric symptoms prior to the parasuicide crisis. Conclusion This study painted a clear picture of parasuicidal cases attended at Brits Hospital, being younger between 16-25, mainly female, single, unemployed, the method used for parasuicide mostly overdose of medication and done mainly in Januaries and Februaries. Furthermore, reason for the parasuicidal attempt were relationship problem as well as unemployment. In most of the cases, it was the first attempt.
Systems Biology Unraveled the Relationship of lncRNA OIP5-AS1 with CD25 and its Co-Expression Analysis in Cancers
Authors: Dehbashi Moein , Hojati Zohreh , Motovali-bashi Majid , Cho C. S. , Shimosaka Akihiro , Ganjalikhani-Hakemi Mazdak
Published: August 2020

Background: Treg cells function in the immune homeostasis, these cells express high level of CD25. Even though the molecular mechanisms of CD25-mediated signaling pathways has been reported, some questions are still unclear, e.g. the relationship and function of the relative lncRNA. It is known that the CD25 expression levels are various among different cancers. Thus, we intended to dissect systems biology of a lncRNA pertained to CD25 and CD25 protein interactors-targeting miRNAs. Methods: Apart from using the available RNA-seq data, the co-expression analysis of the lncRNA pertained to some cancers was performed. Our analysis was done for protein interactors of CD25 by STRING 11.0, ShinyGO v0.60 and KEGG web servers were used for enrichment and network analysis of CD25. TargetScan 7.2, miRTargetLink Human and mirDIP were applied for determining the CD25 and CD25 interactors-targeting miRNAs. To find the lncRNA-miRNA and lncRNA-protein interactions, starBase v3.0, LncBase Predicted v.2 and SFPEL-LPI were recruited, respectively. Also, using Co-LncRNA, the co-expressed lncRNA analysis and the relative signaling pathways in some cancers including bladder, breast, head and neck, kidney, liver, lung, prostate and thyroid cancers using RNA-seq data were achieved. Results: OIP5-AS1 was shown to have the interaction with CD25 and CD25 protein interactors-targeting miRNAs. In addition, the co-expression of OIP5-AS1 in cancers and their signaling pathways was identified. Conclusions: Possibly, OIP5-AS1 can effect on CD25 expression in all relative signaling pathways of these cancers.
Verification of virtual reality to evaluate deficiencies in cognitive function among patients with schizophrenia in the remission stage: a cross-sectional study
Authors: Huang Manli , Li Shangda , Sun Bing , Lyu Hailong , Xu Weijuan , Jiao Jianping , Pan Fen , Hu Jianbo , Chen Jinkai , Chen Yaping , Yi Xu
Published: August 2020

Background: Schizophrenia is associated with widespread cognitive impairments. The MATRICS Consensus Cognitive Battery (MCCB) is most frequently used to assess cognitive function. However, the MCCB test can be time consuming for the clinician. Virtual reality (VR) has emerged as an adjunctive tool to overcome this limitation, which provides a new means of assessing cognitive function. Methods: The study is to explore the validity and safety of using VR technology to assess cognitive function in Han Chinese patients with schizophrenia. The virtual reality supermarket training system (VRSTS), which simulates real-life supermarkets, was used to assess cognitive function. Thirty-two patients with schizophrenia (SZs) and 25 healthy controls (HCs) received the VRSTS and MCCB assessments. A diagnosis model was created based on the outcomes of VRSTS to classify SZs and HCs by cognitive impairment. Results: Significant differences in completion time between the SZs and HCs were detected on the VRSTS. SZs spent more time completing tasks than HCs. The outcome of VRSTS was significantly correlated with the MCCB. Moreover, the diagnosis model has a sensitivity of 88.89% and a specificity of 88.89%. Conclusions: These results provide support for VR technology in the assessment of cognitive impairment in schizophrenia among Han Chinese patients.Trial registration: China Clinical Trial Registry, ChiVTR1800016121. Registered 13 May 2018, http://www.chictr.org.cn/showproj.aspx?proj=27233
The subchronic toxic effects of Mosla Chinensis Maxim in normal rats.
Authors: Lei Dan , Li Longxue , Huang Shenghong , Liu Li , Cai Pingdong , Gu Zhouyang , Shu Kun , Li Shouming , Liu Zhiyong
Published: August 2020

Background: The aim of this work was to study the toxic effects and target organs of Mosla Chinensis Maxim (MCM) in rats and provide theoretical basis for clinical medication.Methods: The subchronic toxicity study was conducted on 60 male and female SD rats using the fixed-dose method for the treatment group and 20 male and female SD rats for the control. At the subchronic toxicity study, the water extract of MCM with fixed-dose of 0.2g/kg/day, 2g/kg/day and 20g/kg/day was administered for 90 days intragastric, and the control group was given the same amount of distilled water. After 90 days, the general conditions of the rats were observed. Assesment on safety of the extract was conducted by a subchronic toxicity test which mainly examined alteration occured in gut flora and urine metabolism. Results: The results showed that there were no significant toxic effects observed at all doses on physical sign and reactivity and fecal property of rats in the treatment groups had no obvious difference from those in control group. The results of routine blood test showed that the number of red blood cells in the male medium dose group and the female low dose group were significantly different from those in the control group (P<0.05). The results of serum biochemical indicators test showed that MCM had influence on the indicators of liver and kidney function, but it had no toxicological significance. In terms of glucose and lipid metabolism, the LDL level of male rats was lower than that of the control group (P<0.05). Compared with the control group, GLU level of female rats in the low, medium and high dose groups was significantly increased (P<0.05), indicating that long-term administration of MCM would affect the glucose level of female rats. The results of intestinal flora diversity showed that feeding MCM for 90 days had an impact on the distribution of intestinal flora. The content of lactobacillus increased and the ratio of Firmicutes and Bacteroidetes (F/B) was also affected, but there was no significant difference. Conclusions: These findings showed that the long-term intragastric administration of the MCM is safe to use within its dose recommendation. But it could have slight affect the metabolism of uric acid by changing the composition of intestinal flora and affecting the metabolism of tryptophan.
A Quantitative Discriminant Method of Elbow Point for the Optimal Number of Clusters in Clustering Algorithm
Authors: Shi Congming , Wei Bingtao , Wei Shoulin , Wang Wen , Liu Hai , Liu Jialei
Published: August 2020

Clustering, as a traditional machine learning method, is still playing a significant role in data analysis. The most of clustering algorithms depend on a predetermined exact number of clusters, whereas, in practice, clusters are usually unpredictable. Although elbow method is one of the most commonly used methods to discriminate the optimal cluster number, the discriminant of the number of clusters depends on manual identification of the elbow points on the visualization curve, which will lead to the experienced analysts not being able to clearly identify the elbow point from the plotted curve when the plotted curve being fairly smooth. To solve this problem, a new elbow point discriminant method is proposed to work out a statistical metric estimating an optimal cluster number when clustering on a dataset. Firstly, the average degree of distortion obtained by Elbow method is normalized to the range of 0 to10; Secondly, the normalized results are used to calculate Cosine of intersection angles between elbow points; Thirdly, the above calculated Cosine of intersection angles and Arccosine theorem are used to compute the intersection angles between elbow points; Finally, the index of the above computed minimal intersection angles between elbow points is used as the estimated potential optimal cluster number. The experimental results based on simulated datasets and a public well-known dataset demonstrated that the estimated optimal cluster number output by our newly proposed method is better than widely used Silhouette method.
Hidden Pulmonary Arteries in Tetralogy of Fallot and Pulmonary Artery Pressure in Patients Operated with a Pulmonary Artery
Authors: Edraki Mohammadreza , Ghasemzadeh Bahram , Keshavarz Kambiz , Amirghofran Ahmadali , Mohammadi Hamid , Kheirandish Zahra , Amoozgar Hamid , Ajami Gholamhossein , Mehdizadegan Nima , Naghshzan Amir , Peiravian Farah , Cheriki Sirous , Nobahkti Mohammadjavad
Published: August 2020

Introduction: The absence of a pulmonary artery is a rare congenital anomaly that occurs on its own or with some congenital cardiac disorders, particularly tetralogy of Fallot (TOF), while the hidden pulmonary artery might originate from a closed ductus arteriosus (DA) that can be stented to reach the artery.Material and methods: This prospective study describes cardiac catheterization of our nine TOF patients who had the absence of the left pulmonary artery before the operation. The patients were stratified in three groups: group one, whose closed DA were found and stented successfully to the hidden pulmonary artery; group two, whose hidden pulmonary arteries were found via the pulmonary vein angiography; and group three, for whom we could not find the remnant of the DA, or our attempt to stent the DA to the hidden pulmonary artery was not successful.We also evaluated outcomes of the other surgically-corrected TOF patients who were operated with the absent left pulmonary artery.Results: The first group included patients aged 1, 24, and 30 months, whose CT angiography 6-9 months after stenting showed acceptable left pulmonary artery diameter for surgically correction, while the pulmonary vein angiography of the group two patients showed a hidden left pulmonary artery with a suitable diameter for surgical correction.However, we were unable to find or stent the DA of the group three patients, aged 12, 38, 60, and 63 months. Moreover, evaluation of the other six previously corrected patients who were operated with a right pulmonary artery revealed pulmonary artery hypertension of the entire patients.Conclusion: The concealed pulmonary artery might be found, and stenting of the closed DA to it might be performed to improve the diameter of the diminutive pulmonary artery. This procedure may allow TOF total surgical correction with two pulmonary arteries. Besides, pulmonary vein angiography can reveal the hidden pulmonary artery.
Impact of Cardiac Function Improvement on Cardiac Surgery Associated Acute Kidney Injury for Patients with Preoperative Renal Dysfunction
Authors: Xu Jiarui , Chen Xin , Lin Jing , Li Yang , Shen Bo , Jiang Wuhua , Luo Zhe , Wang Chunsheng , Ding Xiaoqiang , Teng1 Jie , Lv Wenlv
Published: August 2020

Background: We aim to investigate whether the postoperative cardiac function improve or not would affect the risk of cardiac surgery associated acute kidney injury (AKI) for patients with preoperative renal dysfunction. Method: Data from patients underwent cardiac surgery from April 2012 to February 2016 were collected. Renal dysfunction was defined as preoperative SCr >1.2 mg/dL (females) or >1.5 mg/dL (males). Patients were grouped as normal renal function group, renal dysfunction with chronic kidney disease (CKD group), and non CKD group. △LVEF=postoperative LVEF - preoperative LVEF. Cardiac function improved was defined as △LVEF ≥10. Patients were further divided into non CKD & cardiac function improved (non CKD+), non CKD & cardiac function not improved (non CKD-), CKD & cardiac function improved (CKD+) and CKD & cardiac function not improved (CKD-) subgroups.Results: A total of 8,661 patients were allocated as normal renal function (n=7,903), non CKD(n = 662) and CKD (n = 136) groups. Both non CKD and CKD groups had higher AKI incidence than normal function group (39.5% vs 30.0%, P < 0.001; 61.8% vs 30.0%, P<0.001), and non CKD+ group had the similar AKI incidence with normal function group (30.9% vs 30.0%, P=0.729). Multivariate logistic regression analysis revealed that non CKD-, CKD+ and CKD- were significant risk factors, whereas non CKD+ was not a significant risk factor for postoperative AKI. The SCr at discharge in non CKD+ subgroup was significantly lower than its preoperative SCr (1.4 ± 0.8 vs 1.7 ± 0.9 mg/dL, P = 0.020).Conclusions: For renal dysfunction patients with no CKD, the risk of postoperative AKI did not exist if the cardiac function improved after surgery. For CKD patients, the risk of postoperative AKI increase regardless whether the cardiac function improved or not.
IgG4-Related Disease as Mimicker of Malignancy
Authors: Zhou WanLi , Murray Timothy , Cartagena Liliana , Lim Howard , Schaeffer David F. , Slack Graham W. , Skinnider Brian F. , Lam Eric , Irvine Robert , Chung Stephen , Mattman Andre , Chang Silvia , Carruthers Mollie N. , Chen Luke Y.C.
Published: August 2020

Background: IgG4-related disease is an immune-mediated disease that may present as a tumefactive lesion in nearly any organ. These mass lesions often resemble malignancy both clinically and radiologically, and some patients undergo unnecessary surgical resection. We performed a retrospective single center study examining how many patients with IgG4-RD were initially believed to havemalignancy,with particular attention to those who underwent potentially avoidable surgical procedures. Methods: 63 patients with biopsy confirmed IgG4-Related Disease based on International Consensus Criteria were included. Clinical, laboratory, radiological and histological data were collected and analyzed.Results: Over 60% of patients (38/63) were initially thought to have a malignancywhen they initially presented with symptomatic IgG4-RD. The most common types of malignancy suspected were lymphoma (18/38) and pancreatic cancer (11/38). Of the 38 patients with suspected malignancy, 14 underwent an invasive intervention either to alleviate the severity of their symptoms or as treatment for their presumed malignancy.These included Whipple resection/attempted Whipple (3), nephrectomy (3), bile duct resection and reconstruction (1), removal of other abdominal/retroperitoneal masses (3), and stenting of obstructed organs (4).Conclusion: IgG4-RD should be on the differential diagnosis of patients with mass lesions, in particular those with pancreatic masses and obstructive jaundice, extensive lymphadenopathy, or retroperitoneal masses. Oncologists and other physicians involved in cancer care should be aware of the various manifestations and diagnostic approach to IgG4-RD in order to provide accurate diagnosis and minimize unnecessary invasive procedures.
Land use management and carrying capacity of Bangsri Micro Watershed, East Java, Indonesia: a baseline study
Authors: Dwiastuti Rini , Setiawan Nuri Nurlaila , Aprilia Anisa , Laili Fitrotul , Setyowati Putri Budi
Published: August 2020

The increase of unsustainable human activities in watershed areas gave a huge pressure, causing land degradation and reduced the river water quality. This study aimed to describe the land use management and calculate the carrying capacity as a part of natural resource management of Bangsri Micro Watershed, the upper Brantas watershed in East Java, Indonesia. Data was gathered from five villages through interviews, questionnaires, and focus group discussions with multiple stakeholders. In addition, data from the local government statistics bureau (i.e., Statistics Indonesia) was also used. From the total 2,765 ha land area, eight different land use were identified with agroforestry (970 ha) being the largest and bare land (43 ha) being the smallest. There were 17 crop patterns with 10 different commodities recorded. Based on the present land use, the total land monetary value (biocapacity) was 298 billion Indonesian Rupiah (IDR) or equal to 27,278 tonnes of rice production. From the total population of 27,645; the total annual income (i.e., ecological footprint based on the minimum wage and real household income) needed is 577-854 billion IDR (72,101-106,771 tonnes of rice), thus considered as a deficit carrying capacity value. An urgent watershed management planning is needed to reduce the environmental pressure while ensuring the fulfilment needs of the people in Bangsri Micro Watershed.
Exploration the mechanism of doxorubicin-induced Heart failure in Rats by integration of proteomics and metabolomics data
Authors: Yuan Yu , Fan Simiao , Shu Lexin , Huang Wei , Xie Lijuan , Bi Chenghao , Yu Hongxin , Wang Yuming , Li Yubo
Published: August 2020

Background

Heart failure is currently a worldwide systemic disease with high morbidity and mortality and is very common. At present, many studies have shown that heart failure is associated with obesity, hypertension and diabetes, but it is still unable to prevent the disease from progressing. Here, we elucidate the molecular mechanisms of doxorubicin–induced harmful effects on rat cardiac metabolism and function from a new perspective, using metabonomics and Proteomics analysis data.
Methods

The aim of this study was to use metabonomic and proteomic techniques to systematically elucidate the molecular mechanisms of doxorubicin (DOX)–induced heart failure (HF) in rat. In this study, we aimed to systematically elucidate the molecular action of Dox on rats heart and the reasons for DOX–induced the HF mechanism through the metabonomics tandem mass tag (TMT)–based quantitative proteomics approach. Rats were gavaged with DOX (3 mg/kg) for 6 weeks and the plasma metabonomics, cardiac tissue proteomics, histopathology and related proteins expression levels.
Results

A total of 278 proteins and 21 metabolites were significantly altered in rats following DOX treatments. The responsive proteins and metabolites were predominantly involved in Fatty acid metabolism, Glycolysis, glycerophospholipid metabolism, TCA cycle, Glutathione metabolism, Myocardial contraction.
Conclusions

The present study indicates the PTP1B inhibits the expression of HIF-1α by inhibiting the phosphorylation of IRS, leading to disorders of fatty acid metabolism and glycolysis, which together with the decrease of Nrf2, SOD, Cytc and AK4 proteins lead to oxidative stress, suggesting the PTP1B may serve as a potential target in the treatment of heart failure.
Renalase levels and genetic variants are associated with preeclampsia: a hospital based study in Chinese cohort
Authors: Li Xianshu , Huang Qianqian , Xu Jing
Published: August 2020

Background

Pre-eclampsia (PE) is a major contributor of maternal and fetal morbidity and mortality. There are many host related biomolecules that regulate the pathophysiology PE. Critical analysis of these parameters is of crucial importance as some of these may be used as prognostic/diagnostic marker of this serious ailment and can be targeted for developing therapeutic measures against the disease. The aim of the current study is to examine the role of renalse in the context PE in a Chinese cohort.
Methods

A hospital based case control study was designed to investigate role of renalase in PE. 384 Chinese women consisting of subjects with normotensive pregnancy (n = 105), women with PE (n = 121) and healthy pregnancy (n = 158) were included in the study. Serum renalse level was measured in recruited subjects by ELISA. Renalase gene polymorphisms (rs10887800, rs2576178 and rs2296545) were genotyped by PCR-RFLP.
Results

A higher level of serum renalse in healthy pregnant women compared to controls, whereas, subjects with PE demonstrated a reduced level of this enzyme. Renalse level was negatively correlated with systolic as well as diastolic blood pressure, whereas, a positive association was observed with glomerular filtration rate. Prevalence of homozygous mutant (GG) and minor allele (G) of rs10887800 and rs2576178 polymorphisms were higher in PE patients compared to normotensive pregnant women and healthy controls. Furthermore, association of G-G-C haplotype with susceptibility to PE was observed.
Conclusions

Low level of renalse may be associated with high risk of PE during pregnancy. Renalase gene polymorphisms (rs10887800 and rs2576178) are correlated with with serum renalase and associated with predisposition to development of PE in Chinese cohort.
Elevated Ambulatory Blood Pressure Measurements Are Associated With a Progressive Form of Fabry Disease
Authors: Rossi Federica , Svarstad Einar , Elsaid Hassan , Binaggia Agnese , Roggero Letizia , Auricchio Sara , Marti Hans-Peter , Pieruzzi Federico
Published: August 2020

Background and objectivesPublished data on hypertension incidence and management in Anderson-Fabry disease are scanty and it remains to be shown how much an elevated blood pressure contributes to the patient’s organ damage. Therefore, we have assessed blood pressure values and their correlations with clinical findings in a cohort of Fabry patients.MethodsBetween January 2015 and May 2019, all adult Fabry patients (n = 32; n = 24 females, n = 8 males) referred to our institute were enrolled. Data regarding hypertension were obtained by ambulatory blood pressure monitoring, home self-monitoring and repeated office measurements. Organ involvement and hypertension risk factors were also evaluated.ResultsThe ambulatory monitoring revealed elevated blood pressure in 18.75% (n = 6) of the Fabry population and 50% of this group was diagnosed with masked hypertension. All these patients were females and they presented a lower glomerular filtration rate and a more advanced cardiac hypertrophy compared with normotensive subjects. They were mostly (66.7%) affected by a progressive form of the disease while the majority of the normotensives (84.6%) were stable. No correlation was found between the category of GLA mutation and the development of hypertension.ConclusionNewly detected hypertension can be found in a restricted portion of stable Fabry patients while it becomes more prevalent among clinically progressive cases. The use of the ambulatory blood pressure monitoring is of paramount importance to reveal masked hypertension which can contribute to the progressive worsening of the organ damage. Therefore, a proper diagnosis and therapy of hypertension may improve the outcome of Fabry patients.
Inhibitory Effects of Circulating Natural Autoantibodies to CD47-derived Peptides on Oral Squamous Cell Carcinoma Cells
Authors: Liu Xiu , Meng Qingyong , He Ziyi , Dong Rui , Hu Ying
Published: August 2020

Background

Natural autoantibodies serve as an important anti-tumorigenic system due to its immune surveillance function. The present study aims to investigate whether circulating natural IgG autoantibodies against cluster of differentiation 47 (CD47) could inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells.
Methods

The expression of 14 tumor-targeted genes in three OSCC cell lines was analyzed with quantitative real-time PCR. The in-house enzyme-linked immunosorbent assay (ELISA) was then performed to detect plasma IgG antibodies against CD47. Three OSCC cell lines were treated with 20% human plasma positive and negative for anti-CD47 IgG, respectively, followed by analysis of cell proliferation, apoptosis and invasion/metastasis.
Results

CD 47 showed highest expression among all 14 genes detected in three OSCC cell lines. Plasma anti-CD47 IgG significantly inhibited the viability of all three OSCC cell lines. The proportions of apoptotic cells were remarkably higher in OSCC cells treated with anti-CD47 IgG-positive plasma than those treated with IgG-negative plasma. Furthermore, the cell invasion/metastasis was attenuated evidently with the attribution of plasma anti-CD47 IgG.
Conclusions

Natural autoantibodies against CD47 may be a potential target for OSCC immunotherapy.
A case report of moderate COVID-19 with an extremely long-term viral shedding period in China
Authors: wang yonghong , liu chaoyuan , meng qinghui , gui shuang , wu yu , cheng pengjiang , wang peng , liao xiuyong
Published: August 2020

BackgroundAn ongoing outbreak of novel coronavirus disease 2019 (COVID-19) from Wuhan, China, is currently recognized as a global public health emergency, which has subsequently spread to the rest of China and other countries. The WHO raised the COVID-19 alert to the highest level. The virus is a new highly contagious via human-to-human transmission. The median duration of viral shedding is 20.0 days. We report that the longest duration of viral shedding was 32.0 days from illness onset in a patient with moderate COVID-19 admitted to QianJiang Central Hospital.Case presentationA 37-year-old male sought medical advice while suffering from fever, dry cough, fatigue, dizziness, runny nose and diarrhoea. Five days before the visit, he had a history of travel from affected geographic areas. The patient had a positive RT-PCR test, and chest CT images showed multiple nodules and mixed ground-glass opacification with consolidation in both lungs. Laboratory findings showed that his lymphocyte and CD4+ counts were below the normal range. The patient was given antiviral treatment, including arbidol, lopinavir, IFN-α, and traditional Chinese medicine, and other necessary support care. All clinical symptoms and CT imaging manifestation abnormalities resolved during the course of therapy.ConclusionAlthough the positive RT-PCR tests were verified in consecutive upper respiratory specimens, the clinical symptoms, CT imaging findings, CD4 + lymphocyte counts, and IgG antibody levels had obviously improved. Positive tests may be detecting pieces of inactive viruses, which would not be transmissible in individual cases.
COVID-19 Impact on Indian Food and Hospitality Sector with Specific Reference to Potato Crop
Authors: masih jolly , Sharma Mohit , Rajasekaran Rajkumar
Published: August 2020

Disruption through present pandemic is applicable to almost all the sectors of economy. However, impact of COVID-19 pandemic on Indian food, agribusiness and hospitality industry is largely experienced. Further, it is believed that fluctuations in arrivals and price surge of essential staple vegetables like potato can have severe multifold effects on hospitality sector including restaurants and online food delivery platforms. Keeping the vitality of these issues in mind, in present study we decided to keep potato as a focused commodity to be analyzed since it is the most popular vegetable across the globe. Daily data for potato arrivals and prices was considered for the date range between 1st January 2018 to 16th April 2020 using online mandi data from Agmark (government recognized site for vegetable price and arrivals). Arrivals and trends of price behavior over the coming six months during the pandemic was attempted to measure. Time series ARIMA model was adapted for this purpose. Azadpur mandi being the Asia’s largest mandi was purposively chosen as study area. Forecasting range suggested the stress in potato supply chain for coming 6 months also depicted that fluctuation in both prices and arrivals. This study forecasted that the potato price would increase by 58.7% by October 2020 compared to October 2019 and arrivals would be just 3.11% less compared to last year. Situation assessment through forecasted range can draw attention of policy makers to ensure streamlined chain of supply for essentials and preventing any devastating effects of COVID-19 on food and hospitality sector.
Transmission and Control Pressure Analysis of the COVID-19 Epidemic Situation Using Multisource Spatio-temporal Big Data
Authors: Wang Fangxiong , Tan Ziqian , Yao Siqi , Guo Cangfeng
Published: August 2020

Taking the Guangdong-Hong Kong-Macao Greater Bay Area as the research area, this paper used OD cluster analysis based on Baidu migration data from January 11 to January 25 (before the sealing-off of Wuhan) and concluded that there is a significant correlation between the migration level from Wuhan to the GBA and the epidemic severity index. This paper also analyzed the migration levels and diffusivity of the outer and inner cities of the GBA. Lastly, four evaluation indexes were selected to research the possibility of work resumption and the rating of epidemic prevention and control through kernel density estimation. According to the study, the amount of migration depends on the geographical proximity, relationship and economic development of the source region, and the severity of the epidemic depends mainly on the migration volume and the severity of the epidemic in the source region. The epidemic risk is related not only to the severity of the epidemic in the source region but also to the degree of urban traffic development and the degree of urban openness. After the resumption of work, the pressure of epidemic prevention and control has been concentrated mainly in Shenzhen and Canton; the further away a region is from the core cities, the lower the pressure in that region. The mass migration of the population makes it difficult to control the epidemic effectively. The study of the relationship between migration volume, epidemic severity and epidemic risk is helpful to further analyze transmission types and predict the trends of the epidemic.
Formation and dissipation dynamics of the Asian Tropopause Aerosol Layer
Authors: He Qianshan , Ma Jianzhong , Zheng Xiangdong , Wang Yanyu , Wang Yuhang , Mu Haizhen , Cheng Tiantao , He Ruilian , Huang Guan , Liu Dongwei , Lelieveld Jos
Published: August 2020

The Asian Tropopause Aerosol Layer (ATAL) is characterized by enhanced aerosol concentrations in the Asian Summer Monsoon (ASM) anticyclone in the upper troposphere and lower stratosphere at 13-18 km altitude. A growing body of evidence suggests that the aerosol enhancement is closely connected with deep convection during the monsoon. However, the origin of the aerosols is under debate, and the key factors that determine the ATAL variability remain poorly understood. We investigated the formation and dissipation mechanisms of the ATAL and the inter-annual variation from a dynamical viewpoint using satellite observations and meteorological reanalysis data from 2012 to 2018. We identified the northern Bay of Bengal (BoB) and adjacent land area, where air pollution from the Indian subcontinent converges, as the major source area of aerosols to the ATAL. The spatial extent of the ATAL, represented by the mean Attenuated Scattering Ratio from satellite measurements, appears to be related to a secondary circulation driven by the stratospheric Quasi-Biennial Oscillation (QBO). The aerosols are not homogeneously distributed within the ATAL, and descending motion in the western part is found to play an important role in dissipation of the layer. These findings elucidate the ATAL dynamics and associated regional and global air pollution transports.
Neurological manifestations among patients with HIV – active tuberculosis co infection
Authors: Sidig Amira , Taha Mohamed A. , Osman.B.M  , M.Alfaki Musaab , Alfaki Mohamed I. , Alsherif Razeen A. , Abdelrahim Mohamed A. , Yeddi Ahmed. S. , Mohamed.A.Alnor  , A.Kabeer Mohamemd , Abbasher Khabab , Hajnoor Khalid , Malekeladar Mohamed , Abbasher Hussien , Hussien Abbasher
Published: August 2020

Introduction: At least one-third of the 35.3 million people living with HIV worldwide are infected with latent tuberculosis. Tuberculosis is the most common presenting illness among people living with HIV, including those who are taking antiretroviral treatment. There were an estimated 1.1 million HIV positive new TB cases globally in 2012. Around 75% of these people live in sub-Saharan Africa.Despite its great burden, neurological manifestations have not been described yet in patients with HIV-active tuberculosis, although tuberculosis and HIV have synergistic influence on immunity system which may contribute to change in prevalence or severity of CNS involvement in patients with HIV-active TB co infection.Objectives: To study neurological manifestations in patients with HIV-active tuberculosisMethodology: A case series study of 58 consecutive patients with laboratory confirmed HIV- active tuberculosis co infection attending tertiary hospital for tuberculosis treatment was conducted. Data about neurological symptoms and signs – conducted by a neurologist- were collected from each patient. .Results: 24% of 58 patients were found to have neurological manifestations in clinical assessment.Conclusion: the frequency of neurological manifestations among patients with HIV-active TB co infection was found to be higher compared to that of patients with HIV only;
Autoimmune Thyroid Disease Correlates to Islet Autoimmunity on Zinc Transporter 8 Autoantibody: A Cross-Section Study
Authors: Cai Yun , Yan Jieni , Gu Yong , Chen Heng , Hu Qingfang , Chen Yang , Wang Zhixiao , Liu Xiaoyun , Chen Huanhuan , Xu Xinyu , Zhang Mei , Yu Liping , Zheng Xuqin , Yang Tao
Published: August 2020

Background The most common coexisting organ-specific autoimmune disease in patients with Type 1 diabetes mellitus (T1DM) is autoimmune thyroid disease (AITD). Many studies have showed prevalence rate of thyroid autoantibodies range from 3.7-35% in T1DM patients, while some of them suggested the associations between thyroid autoantibodies and islet autoantibodies. However, little work has been done about the anti-islet autoimmune status in patients with autoimmune thyroid disease (AITD), and so far there have been no clinical report based on large population about zinc transporter 8 autoantibody (ZnT8A) in patients with AITD. We aimed to explore the presence of islet autoantibodies, ZnT8A, glutamic acid decarboxylase autoantibodies (GADA) and tyrosine phosphatase autoantibodies (IA-2A) compared with thyroid autoantibodies, thyroid peroxidase autoantibodies (TPOAb) and thyroglobulin autoantibodies (TGAb) and thyrotropin receptor autoantibodies (TRAb) in AITD patients. Methods In total 740 AITD patients, 108 type 1 diabetes mellitus (T1DM) patients with AITD, 172 non-autoimmune thyroid disease (nAITD) patients and 115 healthy controls were recruited in the cross-sectional study. Islet autoantibodies, ZnT8A, GADA, IA-2A and thyroid autoantibodies, TPOAb, TGAb, TRAb were detected with Radioimmunoassay and Chemiluminescence. Islet autoantibody relative value was established to compare the distribution of the three islet autoantibodies. Results The prevalence of ZnT8A and GADA in AITD group was significantly higher than that in healthy controls (ZnT8A: 15.00% vs 1.74%, GADA: 7.97% vs 0.87%, both P<0.05). Similarly, the prevalence of IA-2A in AITD group was higher than that in healthy controls (4.19% vs 0%, P<0.05). However, any islet autoantibodies positive rate in AITD group was significantly lower than that in T1DM with AITD group. Analysis of multivariable linear regression suggested that ZnT8A relative value was positively related with GADA relative value (β=0.352, P<0.01) and TPOAb titer (β=0.002, P<0.01), and GADA relative value was also positively related with ZnT8A relative value (β=0.183, P<0.01). Conclusions An increased prevalence of ZnT8A as well as a relatively high prevalence of islet autoimmunity was found in AITD patients, indicating that there is a potential link between thyroid autoimmunity and islet autoimmunity. Trial registration Retrospectively registered.
Comprehensive Analysis of a 14 Immune-related Gene Pairs Signature to Predict the Prognosis of Gastric Cancer and Immune Features
Authors: Liu Chuan , Chen Bo , Huang Zhangheng , Hu Chuan , Jiang Liqing , Zhao Chengliang
Published: August 2020

Background: As a new method for predicting tumor prognosis, the predictive effect of immune-related gene pairs (IRGPs) has been confirmed in several cancers, but there is no comprehensive analysis of the clinical significance of IRGPs in gastric cancer (GC).Methods: The clinical and gene expression profile data GC patients were obtained from the GEO database. Based on the ImmPort database, differently expressed immune-related genes (DEIRGs) events were determined by a comparison of GC samples and adjacent normal samples. Cox proportional regression was used to construct an IRGPs signature, and its availability was validated with three external validation sets. In addition, we explored the association between clinical data and immune features and established a nomogram to predict outcomes of GC patients. Result: A total of 88 DEIRGs were found in GC from the training set, which formed 3828 IRGPs. 14 overall survival (OS)-related IRGPs were used to construct the prognostic signature. As a result, the patients in the high- risk group have a poorer OS compared with the low-risk group. In addition, the fraction of CD8+ T cells, plasma cells, T cells CD4 memory activated, and macrophages M1 were higher in the high-risk group. The expression of two immune checkpoints, CD276 and VTCN1, was significantly higher in the high-risk group. Based on the independent prognostic factors, a nomogram was established and showed excellent performance.Conclusion: The 14 OS-related IRGPs signature was associated with the OS, immune cells, and immune checkpoints of GC patients, which can provide the basis of related immunotherapy.
Development and Evaluation of Prognostic Nomogram for Patients with Differentiated Thyroid Carcinoma
Authors: Mao Guochao , Lin Shuai , Zhou Zhangjian
Published: August 2020

Background: Papillary thyroid carcinoma and follicular thyroid carcinoma are both well-differentiated thyroid carcinomas. Here, we aimed to establish and evaluate a nomogram for patients with differentiated thyroid cancer.﻿Methods: Patient records were available from SEER database. We enrolled 17,659 patients in total and randomly separated them into a modeling cohort (n = 12,363, 70%) and a validation cohort (n = 5,296, 30%). Predictive models were established via univariate and multivariate Cox regression analysis of potential risk factors and used to produce a nomogram. ﻿Performance of the nomograms in terms of discrimination ability and calibration was evaluated by determining the concordance index (C-index) and by generating calibration plots, respectively, using the internal (modeling cohort) and external (validation cohort) validity.﻿Results: Seven independent prognostic factors (age, race, sex, grade, AJCC T stage, AJCC N stage, and AJCC M stage) were identified and used to develop the nomogram for OS prediction of patients with DTC. The C-index for the modeling cohort was 0.829 (95% CI: 0.807-0.851), and the C-index for the validation cohort was 0.833 (95% CI: 0.803-0.862). Calibration plots of the nomogram indicated acceptable agreement between the predicted 3-, 5-year survival rates and the actual observations in the modeling and validation groups.﻿Conclusions: We have constructed and verified a nomogram containing clinical factors, which showed better prognostic judgment and predictive accuracy for DTC. This will enable clinicians and patients to easily personalize and quantify the probability of DTC during the postoperative period.