bluuebunny/crossref_metadata_2025 huggingface dataset:
The E545 Mutation of PIK3CA in Cervical Cancer Promotes the Survival by Several Different Pathways
Authors: Chen Yan , Yuan Ma-Chi , Shi Jia-Zhen , Zhao Xia , He Nan , Song Qin , Qian Fang-Fang , Wang Hong-Juan
Published: July 2021

Backgroud: The E545 mutation of PIK3CA in Cervical cancer is frequently happened. But the role of E545 mutation of PIK3CA in Cervical cancer is not clear.Methods: In this study, we analysised the molecular signatures of E545 mutation Cervical cancer by bioinformatics methods.Results: We collected transcriptome sequencing results of 227 no mutation cervical cancer tissue samples and 36 mutation cervical cancer tissue samples, then analyzed the data combining bioinformatics methods. A total of 5 differential expression miRNAs were obtained, including 3 up-regulated miRNAs, 1 down-rugulated miRNA. A total of 174 differential expression genes were obtained, including 132 up-regulated genes, 40 down-rugulated genes. GO analysis suggested that the up-regulated DEGs were mainly enriched in transcription factor activity, leukotriene signaling pathway and so on. Besides, we constructed a PPI network with DEGs to screen the top hub genes with a relatively high degree of connectivity. Among them CAV1, KRT20, FOS, had a degree of connectivity larger than 5 and functioned as hub module genes to promote the survival of E545 mutation cervical cancer. We also identified different miRNA-DEG axis, including hsa-mir-449a-AXL, hsa-mir-508-CGA, COL15A1, NNMT, hsa-mir-552-CHST6, NWD1. These axis regulated the survival of E545 mutation cervical cancer togetherly. Conclusions: In conclusion, this study identified DEGs and screened the key genes and pathways closely related to E545 mutation in Cervical cancer by bioinformatics analysis, These results might hold promise for finding potential therapeutic targets of cervical cancer harboring E545 mutation of PI3KCA.
Overexpression of Angiogenic Factors and Matrix Metalloproteinases in the Saliva of Oral Squamous Cell Carcinoma Patients: Potential Non-Invasive Diagnostic and Therapeutic Biomarkers
Authors: Cai Meijuan , Zheng Zhichao , Bai Zhibao , Ouyang Kexiong , Wu Qiuyu , Xu Shaofen , Huang Lihuan , Jiang Yingtong , Wang Lijing , Gao Jie , Pathak Janak L. , Wu Lihong
Published: July 2021

Backgound: Salivary biomarkers hold huge potential for the non-invasive diagnosis of oral squamous cell carcinoma. Angiogenic factors and matrix-metalloproteinases (MMPs) are highly expressed in OSCC tissue, but their expression in the saliva is unknown. This study aimed to analyze the levels of angiogenic factors and MMPS in tumor-tissue and saliva of OSCC patients. Methods: OSCC-tissue, adjacent normal tissue (ANT), saliva from OSCC patients, and healthy controls were obtained. The expression patterns of angiogenic factors and MMPs were analyzed by immunohistochemistry, protein chip array, and RT-qPCR. Results: Results showed higher expression of ANG, ANG-2, HGF, PIGF, VEGF, MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, and TIMP-2 in OSCC-tissues compared to the ANT. Among the overexpressed markers in OSCC-tissues, HGF, VEGF, PIGF, PDGF-BB, MMP-1, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, and TIMP-2 were significantly upregulated in the saliva of OSCC patients compared to healthy controls. Conclusions: The levels of HGF, VEGF, PIGF, PDGF-BB, MMP-1, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, and TIMP-2 were upregulated both in OSCC tissue and saliva of OSCC patients. Bioinformatic analysis revealed the correlation of these factors with patient survival and cancer functional states in head and neck cancer, indicating these factors as possible saliva-based non-invasive diagnostic/prognostic markers and therapeutic targets of OSCC.
Pancreaticoduodenectomy: Impact of Volume on Outcomes at a Tertiary Care Center. Our Experience in Single Institute of Nepal.
Authors: Agrawal Sunit , Khanal Bhawani , Das Ujjwal , Sah Suresh Prasad , Gupta Rakesh Kumar
Published: July 2021

BackgroundPancreaticoduodenectomy is a complex high risk surgical procedure usually done for malignant disease carrying significant postoperative morbidity and mortality. An audit and analysis of rate of postoperative morbidity and mortality and the impact of case volume can provide information about the lacunas in patient care and methods to improve it for safe and early discharge of patients. This study was conducted to find out demographic profile, the rate of perioperative morbidities, mortality and impact of case volume on patients undergoing pancreaticoduodenectomy for malignant disease which may serve as a guide to uplift the patient care in our center.MethodsRetrospective analysis of prospectively collected data of patients undergoing pancreaticoduodenectomy from 2015 to 2019 was performed. A total of 62 patients were included in the study. Patients clinic-demographic details, intraoperative and postoperative events were recorded. Rate of various postoperative morbidities and mortality and year wise trend of these factors were analyzed.ResultsMost of the patients were in sixth decade of life (38.7%) with male preponderance (61.3%). Pancreatic cancer was most commonly seen followed by cholangiocarcinoma (46.8%). SSI (32.3%), intraabdominal collection (25.8%), anastomotic leak (14.5%), pancreatic fistula (22.6%), postpancreatectomy hemorrhage (8.1% ) were the major postoperative events. Mortality was found in 12.9% patients. ConclusionThere has been a decrease in rate of all these postoperative adverse events and improvement in the intraoperative blood loss and surgical duration with advancing years and increasing number of cases.
Quantitative Relaxometry using Synthetic MRI Could be Better than Qualitative T2-FLAIR Mismatch Sign: A Pilot Study
Authors: Kikuchi Kazufumi , Hiwatashi Akio , Togao Osamu , Yamashita Koji , Momosaka Daichi , Kikuchi Yoshitomo , Kuga Daisuke , Hata Nobuhiro , Mizoguchi Masahiro , Yamamoto Hidetaka , Iwaki Toru , Ishigami Kousei
Published: July 2021

Introduction The diagnostic utility of synthetic MRI (SyMRI) in patients with isocitrate dehydrogenase (IDH)-mutant lower-grade gliomas has not been investigated. The purpose of this study was to determine whether quantitative relaxometry using SyMRI could differentiate between lower-grade gliomas with an increased sensitivity when compared to the qualitative T2-FLAIR mismatch sign.Methods Between May 2019 and May 2020, thirteen patients with IDH-mutant diffuse gliomas including seven astrocytomas and six oligodendrogliomas were evaluated. Five neuroradiologists independently evaluated the presence of the T2-FLAIR mismatch sign qualitatively. Inter-rater agreement of T2-FLAIR mismatch sign was calculated using the Fleiss kappa coefficient. SyMRI parameters (T1, T2-relaxation times, proton density [PD]) were measured in the gliomas and compared by Mann–Whitney U-test. The receiver operating characteristic analysis was used to evaluate the diagnostic performance. The simulated T2-FLAIR mismatch sign (%change) was also evaluated.Results The sensitivity, specificity, and kappa coefficient were 57.1%, 83.3%, and 0.38, respectively in T2-FLAIR mismatch sign. There were differences between the gliomas in the 10–90th percentiles and the mean in T1, T2-relaxation times, and PD (all p < .05). The T2-relaxation time of the 10th and 50th percentiles, and the mean showed the highest diagnostic performance (area under the curve = 1.00). The %change of present/absent T2-FLAIR mismatch cases were –49.3 ± 6.5%, –43.4 ± 7.9%, respectively.Conclusion Quantitative relaxometry using SyMRI could differentiate astrocytomas from oligodendrogliomas with an increased sensitivity and objectivity compared to the qualitative T2-FLAIR mismatch sign.
A Systematic Review of Human Papillomavirus Genotypes in Women With Normal Cervical Cytology: The Brazilian Paradigm
Authors: Dom-Chima Ngozi , Biswas-Fiss Esther , Biswas Subhasis
Published: July 2021

Background: Human papillomavirus (HPV) family of viruses is the leading cause of cervical cancer in women worldwide. More than 67 types of HPV are known to infect humans, and their distribution varies from region to region. HPV prevalence studies in Brazil have focused on cervical cancer; however, a detailed understanding of HPV type prevalence in women with normal cervix is absent in the literature. Our primary aim is to systematically review the literature and summarize the prevalence and distribution of HPV types in Brazilian women with HPV positive but normal cervical cytology and lack observed abnormal cells on their cervix's surface upon cytology analysis. Methods: This protocol was designed following the PRISMA-P guidelines. We conducted this systematic review with an active search in PubMed, Web of Science, and CINAHL databases and supplemented by a secondary screening of all included articles' reference lists. The search terms "Brazil", "HPV", "Human Papillomavirus", "prevalence", "distribution", "Human Papillomavirus types", and "normal cervical cytology" were used for screening the databases. Of 1048 articles retrieved and subjected to duplicates assessment, title and abstract assessment, and full-text assessment of eligibility, 11 articles were included in the review. We excluded articles from the male population, known cervical cancer cases, and studies with a sample size of <15. Qualitative assembly of the data and analysis was performed using GraphPad Prism 5.0 Results: The articles included in the study reported the prevalence of HPV types in women with normal cytology and HPV positive from ten Brazilian states. The total sample size ranged from 80 to 432, and the sample size for the HPV positive and normal cervical cytology group ranged from 28 to 209. HPV prevalence ranged from 0 to 89.4%, and a total of 43 HPV types were identified in the study population. There was variation between studies on the distribution of HPV types because of the detection and genotyping technique used and geographical location. HPV66 was the only HPV type detected in every study reviewed, regardless of geographical region and methods. Conclusions: Due to variation in genotyping techniques used in these studies, HPV type prevalence and geographical distribution may be misestimated or underestimated. But results of these studies give a clear view of the total prevalence of HPV types in Brazil. It is also essential to consider the HPV types present in women with normal cervical cytology before the HPV-mediated progression to abnormal cervical lesions.Systematic Review Registration: Prospective Register for Systematic Reviews (PROSPERO) registration CRD42020151655
Salvia Chinensia Benth Inhibits Triple Negative Breast Cancer Progression Through Inducing DNA Damage Pathway
Authors: Wang Kainan , Hu Ye , Xu Lingzhi , Zhao Shanshan , Song Chen , Sun Siwen , Zhao Zuowei , Li Man
Published: July 2021

Background: Salvia chinensia Benth (SCH) is an herb that has extensively demonstrated antitumor attributes in multiple tumors. However, there are only marginal reports about the impacts of SCH on TNBC-triple negative breast cancer tumorigenesis. Herein, our current research reported the in vitro effects as well as the in vivo antitumor effect, and the fundamental mechanisms of SCH on TNBC. Methods: In vitro, we measured the antitumor effect of SCH on TNBC through clonogenic assay, cell viability, flow cytometry, apoptosis assay, migration and invasion assay. Meanwhile, breast cancer xenografts with the aid of MDA-MB-231 cells were established to evaluate the therapeutic effect of SCH on TNBC in vivo. To investigate the molecular mechanisms of SCH, bioinformatic analysis, specifically network pharmacology-based analysis, was performed. Then, the comet assay and western blot analyses were applied to confirm the influence of SCH on the DNA damage and repair pathways.Results: Our results demonstrated that the application of SCH could lead to the reticence of cell proliferation, migration, invasion and the induction of cellular apoptosis caused in TNBC cells. Based on the bioinformatic analysis, we discovered two key compounds of SCH, quercetin and beta-sitosterol, and detected significant synergistic effects of the combined treatment of them on TNBC, especially on the DNA damage of TNBC cells as judged by comet assays. Moreover, substantial inductions of DNA damage were examined after SCH treatment in TNBC cells, as well as quercetin, and beta-sitosterol treatment. Apart from inducing DNA damage, SCH specifically decreased inter-strand crosslink repair pathway and the double-strand breach repair perforated by the homologous recombination pathway. Similar results were also obtained with quercetin and beta-sitosterol treatment. Conclusion: Taken together, the current investigation elucidates the novel discernment into the therapeutic function of SCH as a DNA damage agent for treatment of TNBC.
Conventional Multiplex PCR For Identify Soil Transmitted Helminthes (Ascais Lumbricoides, Trichuris Trichura And Ancylostoma Duodenale) In Fecal Specimens
Authors: Alomashi Ghada Basi Ali , Khudhur Hasan Raheem , Jebur Layla Safar
Published: July 2021

Ascaris lumbricoides, Trichuris trichiura, and Ancylostoma duodenale are soil-transmitted helminths (STHs) and medically neglected in Iraq country in spite of their effect on the public health. A cross-sectional study was performed in the Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Dewanyia province, included 850 tool samples collected from patients who attended to the O&P lab. General stool examination (GSE), Direct wet mount method DWMM and Kato-Katz were using for diagnosis of STH infections through detected the adult and the ovum of the helminthes. A conventional multiplex PCR assay was used for detection of STHs in fecal samples. Base on microscopic examination. The results showed that 275/ 850 range among triple, double and single infection on other hand was 365/ 850 range among triple, double and single infection. In conclusions the investigative sensitivity of DWMM is notable for STH, in exception, it is capable to identify patients with the intention of highest required of management, and therefore contributes to the universal target to reduce STH as a community healthiness trouble.
ANXA2 is Correlated With the Molecular Features and Clinical Prognosis of Glioma, and Acts as a Potential Marker of Immunosuppression
Authors: Ma Kaiming , Chen Xin , Liu Weihai , Yang Yang , Chen Suhua , Sun Jianjun , Ma Changcheng , Wang Tao , Yang Jun
Published: July 2021

Background: Recent studies have shown that ANXA2 plays a crucial role in the development of many cancers, while its role in glioma remains unclear. Thus, our study aimed to investigate the molecular biological characteristics and clinical value of ANXA2 in glioma. Methods: We analyzed the RNA sequencing data of 325 glioma samples from the Chinese Glioma Genome Atlas (CGGA) as the training cohort and the RNA expression data of 699 samples from The Cancer Genome Atlas (TCGA) database as the validation cohort, totaling approximately 1024 samples. Results: We found that ANXA2 was overexpressed significantly in high-grade glioma (HGG), isocitrate dehydrogenase (IDH) wild-type glioma and mesenchymal-subtype glioma. Receiver operating characteristic (ROC) analysis revealed that ANXA2 was a potential indicator of the mesenchymal subtype. Kaplan–Meier curve analysis revealed that high expression of ANXA2 indicated significantly poorer survival in glioma and HGG patients. Multivariate Cox analysis also demonstrated that ANXA2 acted as an independent indicator of poor prognosis in glioma patients. Further gene ontology (GO) analysis showed that ANXA2-related genes were mainly involved in the innate immune response and inflammatory response of glioma, and these genes were positively correlated with the expression of ANXA2. Then, we selected seven immune-related meta-genes and found that ANXA2 was positively correlated with HCK, LCK, MHC II, STAT1 and interferon but negatively correlated with IgG. Subsequent correlation analysis of infiltrating immune cells showed that ANXA2 was positively correlated with the infiltration of tumor-related macrophages (TAMs), regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs), suggesting that ANXA2 may be involved in the immunosuppression of glioma.Conclusion: Our study revealed that ANXA2 is closely related to the malignant glioma phenotype and poor patient prognosis. ANXA2 also plays an important role in the immune response and inflammatory activity and can be considered a biomarker of immunosuppression in glioma. These findings suggest that ANXA2 may be a promising target for the further development of immunotherapeutic for glioma.
The rich get richer and the poor get poorer: commonness and rarity of arctic tundra plants diverge under warming and herbivore exclusion
Authors: Post Eric , Pedersen Christian , Watts David A.
Published: July 2021

While most species are rare, our understanding of how rare species persist remains limited. Consequently, little is also known about how the commonness and rarity of co-occurring species might be differentially impacted by direct and indirect effects of climate change. We report results of a 15-year field experiment investigating effects on commonness and rarity of 14 arctic tundra plant taxa to warming and exclusion of large herbivores, factors demonstrated to have important effects on plant community composition in many biomes. Across all taxa, pooled commonness was reduced by experimental warming, and more strongly under herbivore exclusion than under herbivory. However, taxon-specific analyses revealed that although warming elicited variable effects on commonness, herbivore exclusion disproportionately reduced the commonness of rare taxa. Over the course of the experiment, we also observed trends in commonness and rarity under all treatments through time. Sitewide commonness increased for two common taxa, the deciduous shrubs Betula nana and Salix glauca, and declined in six other taxa, all of which were rare. Across experimental treatments, rates of increase and decline in commonness (i.e., temporal trends over the duration of the experiment) were strongly related to baseline commonness of taxa early in the experiment. Hence, commonness itself may be a strong predictor of plant species responses to climate change in the arctic tundra biome, but large herbivores may mediate such responses in rare taxa, perhaps facilitating their persistence.
Imaging and reconstruction of the cytoarchitecture of axonal fibres: enabling biomedical engineering studies involving brain microstructure
Authors: Bernardini Andrea , Trovatelli Marco , Klosowski Michal , Pederzani Matteo , Zani Davide , Brizzola Stefano , Porter Alexandra , Baena Ferdinando Rodriguez y , Dini Daniele
Published: July 2021

There is an increased need and focus to understand how local brain microstructure affects the transport of drug molecules directly administered to the brain tissue, for example in convection-enhanced delivery procedures. This study reports the first systematic attempt to characterize the cytoarchitecture of commissural, long association and projection fibers, namely: the corpus callosum, the fornix and the corona radiata. Ovine samples from three different subjects were stained with osmium tetroxide (to enhance contrast from cell organelles and the fibers), embedded in resin and then imaged using scanning electron microscope combined with focused ion beam milling to generate 3D volume reconstructions of the tissue at subcellular spatial resolution. Particular focus has been given to the characteristic cytological feature of the white matter: the axons and their alignment in the tissue. Via 2D images a homogeneous myelination has been estimated via detection of ~40% content of lipids in all the different fiber tracts. Additionally, for each tract, a 3D reconstruction of relatively large volumes (15μm x 15μm x 15μm – including a significant number of axons) has been performed. Namely, outer axonal ellipticity, outer axonal cross-sectional area and their relative perimeter have been measured. The study of well-resolved microstructural features provides useful insight into the fibrous organization of the tissue, whose micromechanical behaviour is that of a composite material presenting elliptical tortuous tubular fibers embedded in the extra-cellular matrix. Drug flow can be captured through microstructurally-based models, leading to a workflow to enable physically-accurate simulations of drug delivery to the targeted tissue.
Innovation performance of biotechnology firms in Bulgaria: opportunities for enhancing
Authors: Dimitrova Teofana , Yosifov Trayan
Published: July 2021

This paper deals with the investigation of the possibilities to improve the innovation performance in Bulgarian biotechnology firms. The main research purpose is to analyze Bulgaria's position concerning the innovative development of bio-based production. Methodological tools of the research methods were processing quantitative data, content analysis, and comparative situational analysis in combination with dynamic graphic analysis. The results of the research showed that there is a significant deficit in Bulgaria in the innovative development of biotechnology firms. The enhancement of the innovative biotechnological state of Bulgarian firms is a matter of great importance to their successful production and market performance on both the local and international markets. A small number of firms manage to find an adequate solution to creating stable market advantages and face insurmountable difficulties in trying to make it on the global market. Furthermore, Bulgaria's membership in the EU creates additional formal demands for providing a high quality of the production process, putting the local biotechnology firms in an even more challenging position. It suggests that companies need to focus their attention on producing goods with high added value (which is the basis of innovation) and applying and perfecting successful market-oriented approaches. In that relation, the arguments were summarized for the practical benefits of designing a complete model focused on biotechnology firms' market orientation, which is of crucial importance for their long-term innovative development. The study presents an author's conceptual model for measuring innovation performance of biotechnology firms which contains three constructs: market orientation, knowledge integration orientation, R&D effectiveness. The results from the conducted research could be helpful to research and practice R&D developers of firms with an established presence and long market history and upstart enterprises that cannot rely on income from their market activity, market or production experience.
Innovations in achieving sustainable economic performance under income inequality
Authors: Cabelkova Inna , Tvaronaviciene Manuela , Strielkowski Wadim
Published: July 2021

The negative effect of income inequality on economic growth represents a topic that constitutes a broad topic of research in the standard economic theory. One of the immediate consequences of income inequality is diminished consumption. Many «poor» customers cannot provide sufficient demand for the producers, causing overproduction that might lead to an economic crisis. It constitutes a problem because sustainable economic performance needs to be achieved under the conditions of income inequality. Reducing social and economic inequality in countries is an essential step towards ensuring that no one is left behind. It is also part of the 10th Sustainable Development Goal aimed to reduce it by 2030. Inequality is based on the income distribution between the top 1% and the bottom 99% of households in any given country. The degree of inequality could play a beneficial role if it is driven by market forces and is associated with incentives to increase growth. In developing and emerging countries, greater equality and improvements in living standards are needed to enable populations to flourish. Inequality reduction is one of the most critical steps a government could take to improve the well-being of its population. The income inequality growth increases human capital in poor countries and reduces it in high and middle-income countries. In poorer countries, it increases them, but in higher – and middle-income countries, it reduces them. Income inequality could be reduced by improving human capital and general skill levels, correcting labor-market policies, and making better use of financial services. In turn, sustainable economic growth could reverse the negative effects of inequality, reducing the need for high-wage and higher-earning households. Thus, it provides higher economic growth. This paper discusses three ways to circumvent the impact of decreasing consumption on economic growth adopted in developing economies over the last fifty years, such as increasing exports, providing loans for consumption, and printing new money. The findings showed that none of these methods seem to be sustainable in the long run. Thus novel and innovative mechanisms that would allow our economy to reduce inequality are necessary and need to be put into place.
Optimization of e-commerce distribution center location
Authors: Kostikov Eva , Jílkova Petra , Kotatkova Stranska Pavla
Published: July 2021

Since the COVID-19 pandemic hit last year, countries locked their borders. Thus, international shipping deteriorates drastically. Simultaneously, social distancing increased the need for immediate online consumption and fast home delivery. In the non-digital world, products still need to be shipped to their destination using trucks, trains, airplanes, and ships. Simultaneously, requirements for volumes of goods, transport costs, external limiting factors, etc., must be precisely defined. The article aims to find the optimal location selection solution based on the created mathematical model of the Modified Steiner-Weber Problem with restrictive conditions. The model allows for the central warehouse's optimal location and minimizes distribution costs from the central warehouse to sub-warehouses/branches located in individual EU countries. The mathematical model has been applied to a case study of a selected e-commerce dealing, which has established branches in capital cities but does not have an established central warehouse. Systematization of literature sources and approaches to solving the problem of e-commerce distribution center location showed that 86% of the studied companies plan to use on-demand warehousing in the next three to five years. Therefore, the need for warehousing would be preserved. The authors noted that they do not necessarily need to have it in-house. Consequently, fulfillment centers and warehouses would likely continue to be a significant component in the future logistics system. This research would like to stress how important the management of the effective optimization of e-commerce distribution center location is and how to achieve it. The success of Amazon in the US, Europe, and Alibaba in China has genuinely redefined consumer expectations. With the emergence of services like Amazon Prime, consumers now expect same-day delivery. The solution enabling this evolution has been a mix of manufacturing where the production costs are optimal, just-in-time shipping, highly automated fulfillment centers, and mobile connectivity growth. The proposed model results showed that the best location for a central location and storage center concerning the e-commerce environment, including minimum annual transport costs, is near Bristol in the United Kingdom. Eighty-six percent of the companies in the study plan to use on-demand warehousing in the next three to five years, and the solution enabling this evolution has been a combination of manufacturing where the production costs are optimal, just-in-time shipping, highly automated fulfillment centers, and, to a growing extent, mobile connectivity.
Relay Cropping Bell Pepper and Tomato: Effects of Cropping Sequence and Transplanting Date
Authors: Sandhu Ravneet K. , Boyd Nathan S. , Zotarelli Lincoln , Agehara Shinsuke , Peres Natalia
Published: July 2021

Vegetable growers in Florida face rising production costs, reduced crop value, and competition from foreign markets. Relay cropping is a variant of double cropping, where the second crop is planted into the first crop before the harvest is finished. This cropping system may be a potential solution to lower production costs per crop by sharing some inputs for two crops. The objectives of this study were to determine the effect of cropping sequence and transplanting date of the secondary crop when relay cropping tomato and bell pepper. Two field experiments were conducted at the Gulf Coast Research and Education Center in Balm, FL, in 2018 and 2019. In the first experiment, tomato was grown as the primary crop and bell pepper was added as the secondary crop, with multiple transplanting dates (8 Aug., 23 Aug., 7 Sept., and 24 Sept.). The second experiment had the same setup but the reverse cropping sequence. Bell pepper yield as the secondary crop was reduced by 65% when grown with tomato as the primary crop compared with bell pepper planted alone. Transplanting date had no effect on bell pepper yield (P = 0.091). Tomato yield was unaffected by the presence of the secondary crop. In the second experiment, tomato yield as a secondary crop was 36% lower when grown with bell pepper as the primary crop compared with tomato crop alone (monocropped). However, tomato yield was significantly reduced by the presence of bell pepper only when tomato crop was planted within 30 to 45 days after planting bell pepper. Based on these results, we recommend relay cropping tomato as the secondary crop within 30 days of planting of bell pepper as the primary crop. However, we do not recommend relay cropping bell pepper as the secondary crop with tomato.
Weed Suppression, Nitrogen Availability, and Cabbage Production Following Sunn Hemp or Sorghum-sudangrass
Authors: Besançon Thierry E. , Wasacz Maggie H. , Heckman Joseph R.
Published: July 2021

Cover crops included in a crop rotation can help increase nitrogen (N) availability to subsequent crops, raise soil organic matter, and suppress emergence and growth of various weed species. However, weed suppression by cover crops has mostly been investigated shortly after cover crop termination and not over a longer period spanning into the next cropping season. The effects of sunn hemp (Crotalaria juncea) and sorghum-sudangrass (Sorghum ×drummondi) planted the previous year on N availability before transplanting of late summer cabbage (Brassica oleracea), weed germination and growth, and cabbage yield was examined in field studies conducted in 2018 and 2019 at Pittstown, NJ. Results established that there was little evidence for a functional difference in soil N availability for fall cabbage production because of previous cover crop type. Heavy rainfall events both years may have caused major losses of available N that might otherwise be expected to come from N mineralization of residues of legume cover crop like sunn hemp. During the cover crop season, smooth pigweed (Amaranthus hybridus) and common lambsquarters (Chenopodium album) dry biomass was 77% and 82% lower, respectively, in sorghum-sudangrass compared with sunn hemp plots. The subsequent season following sorghum-sudangrass cover crop, dry biomass of broadleaf weeds was lower by 74% and 56% in June and July, respectively, compared with preceding sunn hemp. Smooth pigweed, common lambsquarters, and hairy galinsoga (Galinsoga quadriradiata) were the weed species most consistently affected by preceding sorghum-sudangrass cover crop with biomass decreased by up to 80%, 78%, and 64%, respectively. Thus, it appears that sorghum-sudangrass can provide suppression of some broadleaf species over a relatively long period and is indicative of sorghum-sudangrass allelopathic activity. On the contrary, density and biomass of grassy weeds as well as commercial yield of transplanted cabbage were unaffected by the preceding cover crop. These results suggest that sorghum-sudangrass cover crop could be integrated to transplanted cole crop rotation for providing weed suppression benefits without altering crop yield in New Jersey organic vegetable cropping systems.
A IMPORTÂNCIA DA INSERÇÃO DA CULTURA DA PAZ NO CURRÍCULO ESCOLAR
Authors: Monteiro Michelle Popenga Geraim , Lima-Berton Tatiane Delurdes de , Asinelli-Luz Araci
Published: July 2021

O objetivo do presente estudo é salientar a importância da inserção da Cultura da Paz no currículo escolar para a prevenção das violências na infância no ambiente escolar, visto que a escola tem sido considerada como espaço de expressão e manifestação da violência.  É resultado de um estudo qualitativo e tem como metodologia o estudo bibliográfico, decorrentes de pesquisas de abordagem teórica, relacionadas ao Mestrado em Educação da Universidade Federal do Paraná. No contexto social e educacional, em defesa de uma escola com base humanizadora, amplia-se o olhar para o âmbito do desenvolvimento do currículo escolar, aliado à Cultura da Paz como forma de compreensão e prevenção do fenômeno das violências. Acredita-se que ao compreender a escola como espaço de interações, cujos valores são pautados no diálogo e no respeito, aumenta-se a chance concreta de uma realidade educativa melhor e de prevenção das violências.
CURSO NORMAL RURAL DO COLÉGIO NOSSA SENHORA AUXILIADORA DE PETROLINA (1929-1989): UM MARCO NA HISTÓRIA DA FORMAÇÃO DOCENTE
Authors: Mendes da Silva Rosa Santos , Reis Edmerson dos Santos
Published: July 2021

RESUMO
Neste trabalho apresentamos uma trajetória histórica do Curso Normal Rural do Colégio Nossa Senhora Auxiliadora. Essa Instituiçãose enquadra dentro de uma perspectiva de ensino confessional, mantida pela congregação das filhas de Maria Auxiliadora, constituída nos conceitos cristãos fundada na primeira metade do século XX.Com relação aos aspectos teórico-metodológicos, a pesquisa baseou-se na análise histórico documental e pesquisa de campo, tendo como base o recorte temporal as décadas de 1929 a 1989, período que abrange desde a criação do curso normal rural até o ano em que houve oficialmente a extinção do curso.  Foi possível apreender que no estado de Pernambuco a primeira “Escola Normal Rural” foi a do Colégio Nossa Senhora Auxiliadora no ano de 1936. Assim, a educação do Curso Normal esteve associada às idéias de cristianização da Igreja Católica e atendeu os anseios da sociedade da época.
PALAVRAS-CHAVE: Curso Normal Rural.Educação Confessional.  Formação docente.
REPRESENTAÇÕES SOCIAIS SOBRE A EDUCAÇÃO ESPECIAL/EDUCAÇÃO INCLUSIVA: REVISÃO DA LITERATURA (2008-2018)
Authors: Guimarães Orliney Maciel , Nagatomy Gabrielly Fuji Messias
Published: July 2021

Este artigo trata-se de uma revisão sistemática de literatura sobre as Representações Sociais (RS) sobre a Educação Especial (EI)/Educação Inclusiva (EI) referente ao período de 2008-2018. A base de dados foi o Catálogo de Dissertações e Teses da Capes e os periódicos do Qualis na área de Educação. A busca totalizou 60 produções e foi possível constatar que essas pesquisas vêm crescendo nos últimos anos, as quais têm sido produzidas em todas as regiões do Brasil, e que foram realizadas em universidades públicas, estaduais e privadas. A maior parte das produções emprega a abordagem qualitativa com pesquisa de campo e, para a constituição e análise de dados, utilizam-se de entrevistas e da Análise de Conteúdo. Os participantes das pesquisas são, em sua maioria, professores em formação inicial e do ensino superior. Foi possível constatar que os objetivos das pesquisas abordam as RS sobre os processos envolvidos na inclusão de alunos com deficiência ou as RS sobre os aspectos que definem a inserção de um aluno na EE. Os resultados das pesquisas realizadas apontaram, em sua maioria, que as RS não favorecem a inclusão e que as principais barreiras são a acessibilidade atitudinal e a de procedimentos educacionais. Esta revisão endossa a relevância da TRS para estudos sobre EE/EI.
DESENVOLVENDO O PENSAMENTO ABSTRATO NO ENSINO DE FÍSICA POR MEIO DO LABORATÓRIO ABERTO
Authors: Campos José Galúcio
Published: July 2021

Este trabalho é um ensaio em que se apresentam resultados positivos sobre o desenvolvimento da habilidade cognitiva de pensamento abstrato, no ensino de física, por meio do laboratório aberto. Para tanto realizou-se uma pesquisa qualitativa do tipo pesquisa-ação (aproximadamente) onde se utilizou de diversos instrumentos de coleta e análise de dados. Os sujeitos da pesquisa foram os alunos do 2º ano do ensino médio integrado, professores de física, coordenadores e chefes de departamento.
MATHEMATICAL MODELING OF THE SPREADING OF GENERATIONS OF INDUSTRIAL PRODUCTS IN A COMPETITIVE MARKET
Authors: BRAND Albert E. , YAKUBOVSKIY Yuriy E.
Published: July 2021

This article studies the process of spreading generations of industrial products in the competitive market and assessing the influence of the characteristics of generations of products and destabilizing factors on the volume of their sales. The level of innovation and competitiveness of generations is used as characteristics, their definition and mathematical formalization are given. The study uses the generalized model of F. Bass, the provisions of the concept of “multi-product competition” by R. Peterson and V. Mahajan, and the concept of the va­riability of consumer behavior of different generations by T. Islam and N. Mead. A model of the spreading of generations of industrial products of competing brands in the duopole market is obtained, taking into account destabilizing factors. Based on this model, equations are constructed that establish the relationship between the shares of consumers of competing generations of pro­ducts. The statistical data on the spreading of generations of video game consoles from Sony and Microsoft in the global and regional markets serve as a basis for approbation. To identify the parameters of the model and determine the presence and closeness of the relationship, correlation-regression analysis and the least squares method are used. These results demonstrate a high level of correlation between the sales volume of each console generation and the characteristics of the console generations. It was found that with an increase in the influence exerted by a competitor, the cumulative market share of the considered generation of the product decreases, and with an increase in the level of innovation of the generation of the product, its level of competitiveness increases. The obtained results of processing the predicted and actual data on the spread of generations demonstrate a significant influence of destabilizing factors on the process of spreading generations. The theo­retical significance of the work consists in the development of a model for the distribution of generations of industrial products for a particular case with a duapole market structure. The practical significance lies in obtaining the calculated values of the link between the sales volume of each generation of consoles and their characteristics.
Low Total Lymphocyte Count as the Risk of Hospital Acquired Malnutrition in Children
Authors: Ekaputri Dian Sulistya , Sidiartha I Gusti Lanang , Pratiwi I Gusti Ayu Eka
Published: July 2021

Background: Hospital Acquired Malnutrition (HAM) is characterized by inadequate nutritional therapy and the risk of developing malnutrition during the hospital stay. In clinical practice, there are many measurements to determine nutritional status. Total lymphocyte count (TLC) is associated with impaired function of immune system in malnutrition. The purpose of this study was to evaluate the prognostic value of TLC to the occurrence of HAM in pediatric patients.Materials and Methods: This an observational study with a prospective cohort design. Subjects were assessed for weight at the first day of hospitalization, then the subjects were followed until they were discharged. Body weight was re-measured on discharge to determine the presence or absence of HAM. This research was conducted at Sanglah Hospital from May-December 2019. Subjects who met the inclusion and exclusion criteria were enrolled in the study.Results: Among 120 subjects, 55 subjects or 45.8% were malnourished on admission. Subjects with a low TLC compared to a normal TLC had a 3.9-fold risk of experiencing hospital acquired malnutrition (95% Confidence Interval: 1.59 to 7.19, p=0.001). Subjects who had a low TLC had HAM of 61.8%, while subjects who had a normal TLC had HAM of 32.3%. In multivariate analysis, low TLC was the only risk factor for HAM in this research.Conclusion: This study proved that low TLC is the risk of HAM. Total lymphocyte count could be used as predictor of the risk of HAM in hospitalization children.Keywords: hospital malnutrition, total lymphocyte, children
Serum Leptin Concentration is Correlated to Insulin Resistance in Polycystic Ovary Syndrome (PCOS) Patients
Authors: Anwar Arsyi Adliah , Abdullah Nusratuddin , Padjalangi Andi Nursanty , Hamid Firdaus , Mappeware Nasrudin Andi , Lukas Efendi
Published: July 2021

Background: Leptin resistance which leads to excessive circulating leptin levels is thought to affect ovarian function. This study aimed to study the correlation between serum leptin levels with insulin resistance in patients with polycystic ovary syndrome.Materials and Methods: This cross-sectional study was undertaken in several teaching hospitals in Makassar, Indonesia. We included patients diagnosed with polycystic ovary syndrome (PCOS) aged 18-40 years old. Serum leptin levels were examined in all eligible subjects using the enzyme-linked immunosorbent assay (ELISA) method. The results obtained were further analyzed statistically.Results: Approximately 53 PCOS subjects were included in this study, 25 subjects with insulin resistance and 28 subjects without insulin resistance. After examining serum leptin levels, we found that leptin is directly proportional to insulin resistance (p<0.001). We even found a strong positive correlation between serum leptin levels with homeostatic model assessment for insulin resistance (HOMA-IR) levels (r=0.659; p<0.001). Leptin was found to be independent of HOMA-IR, not influenced by confounding factors such as body mass index (BMI) (p=0.090).Conclusion: There was a significant correlation between serum leptin levels and HOMA-IR values in PCOS patients. This correlation was found to be significant regardless of patient's BMI, therefore is considered to have a direct effect on insulin resistance in PCOS.Keywords: polycystic ovary syndrome, leptin, insulin resistance, HOMA-IR
Influence of Metabolic Syndrome on Factors Associated with Chronic Kidney Disease: A Cross-sectional Study
Authors: Marreiros Camila Santos , Nogueira Thaís Rodrigues , do Nascimento Paulo Pedro , de Araújo Diana Stefany Cardoso , do Nascimento Monteiro Nayara Vieira , da Paz Suzana Maria Rebelo Sampaio , de Jesus e Silva de Almendra Freitas Betânia
Published: July 2021

Background:

Metabolic Syndrome (MetS) is defined by the presence of three or more
of the following components: inadequate fasting serum glucose levels and elevated waist circumference,
hypertension and dyslipidemia, which represent a potential risk for the development
and/or worsening of Chronic Kidney Disease.

Objective:

Based on this, this research aimed to investigate the presence of MetS and its influence
on associated factors in patients with Chronic Kidney Disease undergoing hemodialysis.

Methods:

This is an evaluation of a cross-sectional multicenter research project, carried out with
95 patients with Chronic Kidney Disease, seen at outpatient clinics in the state capital Piaui. Anthropometric,
biochemical and hemodynamic parameters were determined. The data were analyzed
using the Stata® v.12 software (Statacorp, College Station, Texas, USA), adopting a significance
level of p < 0,05. The survey received ethical approval (no. 2.527.329).

Results:

It was observed that individuals with elevated BMI, WC, NC, SBP, DBP were more
likely to develop MetS, with significant differences (p <0.001). In addition, it was found that
serum levels of glucose, insulin, HOMA-IR, TC, LDL, TG and blood pressure were higher in the
group with MetS.

Conclusion:

It was concluded that changes in the parameters analyzed in patients with CKD reinforce
MetS as a predictive condition for worsening nutritional status and a factor for the progression
of kidney disease.
The Use of Mobile Phones by Women Accessing Health Care Services in
Bangladesh: A Cross-Sectional Study
Authors: Hossain Md. Mosharaf , Min Ruhani Mat , Shukri Madihah Mohd
Published: July 2021

Background:

Maternal and neonatal mortality rates still pose a global challenge.

Objectives:

The present study aimed to identify the socio-demographic characteristics of mobile users trying to access antenatal care services, transportation to facilities, and postnatal care for mothers and newborns.

Methods:

This was a cross-sectional study that used data collected from the Bangladesh Demographic and Health Survey (BDHS) 2014. Data were gathered using a questionnaire. Participants comprised 4,494 mothers aged between 14 and 49. Models of logistic regression were used to determine associations.

Results:

The overall sample size was 4494, with only 22.23% of women using mobile phones. Among these, 29.9% used their mobile phones to obtain health services or advice and 70.1% did not. Women with mobile phones who had a higher level of education used them to receive more health care (secondary and higher: OR = 1.922, 95%CI = 1.225−3.014; primary: OR = 1.982, 95% CI = 1.738−2.654); they were of higher socio-economic status (rich: OR = 1.228, 95% CI = 1.009&−1.494; middle income: OR = 1.691, 95% CI = 1.256−2.333); they accessed more prenatal care (yes: OR = 1.993, 95% CI = 1.425−1.987) and antenatal care (yes: OR = 1.951, 95% CI = 1.765−1.998); they checked their health status after delivery (yes: OR = 1.966, 95% CI = 1.639−2.357).

Conclusion:

The results of the present study showed that young women who had a higher level of education and income and resided in urban areas were more likely to use a mobile phone to receive health care facilities in Bangladesh. Intervention studies are essential in recognising that mobile phone-based facilities improve maternal health care.
PEMBUATAN MODUL PEMBELAJARAN AUTOCAD PADA MATA PELAJARAN APLIKASI PERANGKAT LUNAK DAN PERANCANGAN INTERIOR GEDUNG DI SMK NEGERI 3 YOGYAKARTA
Authors: Aldo Ahmad
Published: July 2021

Tujuan dari kajian ini adalah untuk mengetahui: (1) tahapan pembuatan modul pembelajaran AutoCAD di SMK Negeri 3 Yogyakarta dan (2) kelayakan modul pembelajaran AutoCAD berdasarkan pendapat ahli materi, ahli media, dan guru. Kajian ini merupakan jenis penelitian pengembangan dengan model 4-D yang terdiri dari empat langkah penelitian. Pengumpulan data dilakukan dengan angket yang didukung kegiatan observasi dan wawancara. Hasil kajian menunjukkan bahwa (1) produk modul pembelajaran dapat disusun dengan proses yang menghasilkan materi mencakup: a) definisi perangkat lunak AutoCAD; b) tampilan dan tahapan pengelolaan file AutoCAD; c) prinsip gambar dua dimensi; d) persiapan menggambar dengan AutoCAD; e) pembuatan gambar detail; f) pembuatan gambar rancangan; g) pembuatan etiket gambar dan pencetakan gambar. Sementara itu, (2) hasil validasi modul berdasarkan ahli materi mendapatkan skor 3,55 dengan kategori sangat layak. Validasi berdasarkan ahli media mendapatkan skor 3 dengan kategori layak. Sedangkan validasi guru mendapatkan skor2,95 dengan kategori layak.