bluuebunny/crossref_metadata_2025 huggingface dataset:
BDNF Val66Met Polymorphism Reduces the Fatigue-Like Effects of 5-Fluorouracil on Voluntary Wheel-Running Activity in Mice
Authors: Wolff Brian S. , Allen Hannah R. , Feng Li Rebekah , Saligan Leorey N.
Published: April 2022

Fatigue is a persistent and debilitating symptom following cancer treatments such as chemotherapy. Recent clinical studies have suggested a common single-nucleotide polymorphism of brain-derived neurotrophic factor (BDNF), Val66Met (rs6265), may be related to the severity of fatigue following cancer treatment. In this study, we tested transgenic mice homozygous for the human Val66Met BDNF gene and wild-type controls. We injected three doses of 5-fluorouracil (5FU) as a model of chemotherapy treatment, and we used changes in voluntary wheel running activity (VWRA) as a measure of fatigue-like behavior. Prior to 5FU injection, we found that during the baseline wheel-running period, the Val66Met mice lost more weight than WT controls. We next administered 5FU and saw a robust fatigue-like phenotype that lasted about 2 weeks. During the first week, the fatigue-like phenotype was less severe in the Val66Met mice and unrelated to the age of the mice. In contrast, during the second week after 5FU treatment, the fatigue-like phenotype was unrelated to the BDNF genotype but was more severe in middle aged mice compared to young mice. We conclude that the BDNF polymorphism may play a direct, protective role against chemotherapy-induced fatigue.
Hippocampus Maintains a Coherent Map Under Reward Feature–Landmark Cue Conflict
Authors: Nair Indrajith R. , Bhasin Guncha , Roy Dipanjan
Published: April 2022

Animals predominantly use salient visual cues (landmarks) for efficient navigation. When the relative position of the visual cues is altered, the hippocampal population exhibits heterogeneous responses and constructs context-specific spatial maps. Another critical factor that can strongly modulate spatial representation is the presence of reward. Reward features can drive behavior and are known to bias spatial attention. However, it is unclear whether reward features are used for spatial reference in the presence of distal cues and how the hippocampus population dynamics changes when the association between reward features and distal cues is altered. We systematically investigated these questions by recording place cells from the CA1 in different sets of experiments while the rats ran in an environment with the conflicting association between reward features and distal cues. We report that, when rewards features were only used as local cues, the hippocampal place fields exhibited coherent and dynamical orientation across sessions, suggesting the use of a single coherent spatial map. We found that place cells maintained their spatial offset in the cue conflict conditions, thus showing a robust spatial coupling featuring an attractor-like property in the CA1. These results indicate that reward features may control the place field orientation but may not cause sufficient input difference to create context-specific spatial maps in the CA1.
Vascular Health Is Associated With Functional Connectivity Decline in Higher-Order Networks of Older Adults
Authors: Wirth Miranka , Gaubert Malo , Köbe Theresa , Garnier-Crussard Antoine , Lange Catharina , Gonneaud Julie , de Flores Robin , Landeau Brigitte , de la Sayette Vincent , Chételat Gaël
Published: April 2022

Background

Poor vascular health may impede brain functioning in older adults, thus possibly increasing the risk of cognitive decline and Alzheimer’s disease (AD). The emerging link between vascular risk factors (VRF) and longitudinal decline in resting-state functional connectivity (RSFC) within functional brain networks needs replication and further research in independent cohorts.

Method

We examined 95 non-demented older adults using the IMAP+ cohort (Caen, France). VRF were assessed at baseline through systolic and diastolic blood pressure, body-mass-index, and glycated hemoglobin (HbA1c) levels. Brain pathological burden was measured using white matter hyperintensity (WMH) volumes, derived from FLAIR images, and cortical β-Amyloid (Aβ) deposition, derived from florbetapir-PET imaging. RSFC was estimated from functional MRI scans within canonical brain networks at baseline and up to 3 years of follow-up. Linear mixed-effects models evaluated the independent predictive value of VRF on longitudinal changes in network-specific and global RSFC as well as a potential association between these RSFC changes and cognitive decline.

Results

We replicate that RSFC increased over time in global RSFC and in the default-mode, salience/ventral-attention and fronto-parietal networks. In contrast, higher diastolic blood pressure levels were independently associated with a decrease of RSFC over time in the default-mode, salience/ventral-attention, and fronto-parietal networks. Moreover, higher HbA1c levels were independently associated with a reduction of the observed RSFC increase over time in the salience/ventral-attention network. Both of these associations were independent of brain pathology related to Aβ load and WMH volumes. The VRF-related changes in RSFC over time were not significantly associated with longitudinal changes in cognitive performance.

Conclusion

Our longitudinal findings corroborate that VRF promote RSFC alterations over time within higher-order brain networks, irrespective of pathological brain burden. Altered RSFC in large-scale cognitive networks may eventually increase the vulnerability to aging and AD.
Co-targeting B-RAF and PTEN Enables Sensory Axons to Regenerate Across and Beyond the Spinal Cord Injury
Authors: Noristani Harun N. , Kim Hyukmin , Pang Shuhuan , Zhong Jian , Son Young-Jin
Published: April 2022

Primary sensory axons in adult mammals fail to regenerate after spinal cord injury (SCI), in part due to insufficient intrinsic growth potential. Robustly boosting their growth potential continues to be a challenge. Previously, we showed that constitutive activation of B-RAF (rapidly accelerated fibrosarcoma kinase) markedly promotes axon regeneration after dorsal root and optic nerve injuries. The regrowth is further augmented by supplemental deletion of PTEN (phosphatase and tensin homolog). Here, we examined whether concurrent B-RAF activation and PTEN deletion promotes dorsal column axon regeneration after SCI. Remarkably, genetically targeting B-RAF and PTEN selectively in DRG neurons of adult mice enables many DC axons to enter, cross, and grow beyond the lesion site after SCI; some axons reach ∼2 mm rostral to the lesion by 3 weeks post-injury. Co-targeting B-RAF and PTEN promotes more robust DC regeneration than a pre-conditioning lesion, which additively enhances the regeneration triggered by B-RAF/PTEN. We also found that post-injury targeting of B-RAF and PTEN enhances DC axon regeneration. These results demonstrate that co-targeting B-RAF and PTEN effectively enhances the intrinsic growth potential of DC axons after SCI and therefore may help to develop a novel strategy to promote robust long-distance regeneration of primary sensory axons.
Structure and Bioactivities of a Novel Polysaccharide Extracted From Dendrobium huoshanense by Subcritical Water
Authors: Wang Li , Mao Yi-gui , Zeng Xiang , Liu Na , Niu Chao-fei , Li Xin-xin , Ma Bing-ji , Guo Lan-ping , Yang Xiao-long
Published: April 2022

In this study, the polysaccharide was extracted by subcritical water from Dendrobium huoshanense. A novel polysaccharide (DHPs-1) was obtained through several purification steps and its structure and bioactivity were investigated. Structural analysis indicated that the weight-average molecular weight of DHPs-1 was 5.0 × 104 Da and it was mainly composed of glucose (65.04%), mannose (14.23%), galactose (8.17%), galacturonic acid (6.41%), rhamnose (2.34%), and xylose (1.25%). 1,4-Glcp, and 1,4,6-Galp were existed in the backbone of DHPs-1. The residues of 1,3,4-Galp, 1,4-Manp, 1,4-Galp, and 1,3,4,6-Galp could be in the backbone or the side chains with the non-reducing terminal of α-Manp. Bioactivity tests indicated that DHPs-1 had immunomodulatory activity in that it significantly enhanced transcript levels of cytokines [Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), and Interleukin-10 (IL-10)]. DPPH and hydroxyl radical scavenging tests showed that it had good antioxidant activity. These results reveal that DHPs-1 could be developed as a safe immunomodulatory agent and antioxidant for pharmacological or functional food applications.
Vesicular Ganglioside GM1 From Breast Tumor Cells Stimulated Epithelial-to-Mesenchymal Transition of Recipient MCF-10A Cells
Authors: Ma Qilong , Zhuo Dinghao , Guan Feng , Li Xiang , Yang Xiaomin , Tan Zengqi
Published: April 2022

Small extracellular vesicles (sEVs) are a type of membrane structure secreted by cells, which are involved in physiological and pathological processes by participating in intercellular communication. Glycosphingolipids (GSLs) are enriched in sEV and can be delivered to recipient cells. In this study, we found that overexpression of B3GALT4, the glycosyltransferase responsible for ganglioside GM1 synthesis, can induce the epithelial–mesenchymal transition (EMT) process in MCF-10A cells. Moreover, GM1 was verified to be presented on sEV from breast cancer cells. Overexpression of B3GALT4 resulted in elevated vesicular GM1 levels and increased sEV secretion in breast cancer cells. Proteomic analysis revealed that eleven sEV secretion-related proteins were differentially expressed, which might contribute to the altered sEV secretion. Of the identified proteins, 15 oncogenic differentially expressed proteins were documented to be presented in sEV. With the treatment of GM1-enriched sEV from breast cancer cells, the EMT process was induced in recipient non-tumorigenic epithelial MCF-10A cells. Our findings demonstrated that GM1-enriched sEVs derived from breast cancer cells induced the EMT process of recipient cells, which might provide essential information on the biological function of vesicular GM1.
The Effective Management of Fever in Pediatrics and Insights on Remote Management: Experts' Consensus Using a Delphi Approach
Authors: Chiappini Elena , Vitale Antonio , Badolato Raffaele , Becherucci Paolo , Careddu Domenico , Di Mauro Antonio , Doria Mattia , Staiano Annamaria
Published: April 2022

Background

Even after the publication of the 2017 update of Italian guidelines on treatment of fever in pediatrics, some fundamental questions are still open and new ones emerged during the COVID-19 pandemic.

Objective

To assess the level of consensus among Italian pediatricians on different topics related to treatment of fever in children by using the Delphi technique.

Methods

A Delphi study was undertaken between June and September 2021, when two questionnaires were consecutively sent to a panel of experts to be answered anonymously. An invitation to participate was sent to 500 pediatricians distributed over the whole national territory and 80 (16%) of them accepted to participate on a voluntary basis. The questionnaires were structured into three specific topics: “therapeutic appropriateness and management of the febrile child,” “management of the febrile child in the presence of other diseases,” and “future perspectives in remote management.” Each topic had six statements.

Results

A first-round questionnaire was sent to 80 accepting pediatricians from different Italian regions. Of the 72 respondents (23% working in hospitals and 72% outside), 33% were from northern, 12% central, and 55% southern Italy or islands. A second-round questionnaire was sent to the same 80 pediatricians and 69 of them responded, without significant differences for workplaces or geographical distribution as compared with the first questionnaire. Overall, 75 participants answered at least one of the two questionnaires. All the statements on the topics of “therapeutic appropriateness and management of the febrile child” and “future perspectives in remote management” reached the predefined cut off for consensus (75% or more). Only one statement on “management of the febrile child in the presence of other diseases” did not achieve the consensus even after the second round.

Conclusions

Italian pediatricians agree on several aspects of treatment of febrile children and their expert opinions could support everyday decision process complementary to recommendations by regulatory agencies and guidelines.
Validation of the Computerized Pediatric Triage Tool, pediaTRI, in the Pediatric Emergency Department of Lenval Children's Hospital in Nice: A Cross-Sectional Observational Study
Authors: Tran Antoine , Valo Petri , Rouvier Camille , Dos Ramos Emmanuel , Freyssinet Emma , Baranton Emma , Haas Olivier , Haas Hervé , Pradier Christian , Gentile Stéphanie
Published: April 2022

Introduction

A reliable pediatric triage tool is essential for nurses working in pediatric emergency departments to quickly identify children requiring priority care (high-level emergencies) and those who can wait (low-level emergencies). In the absence of a gold standard in France, the objective of our study was to validate our 5-level pediatric triage tool –pediaTRI– against the reference tool: the Pediatric Early Warning Score (PEWS) System.

Materials and Methods

We prospectively included 100,506 children who visited the Pediatric Emergency Department at Lenval Children's Hospital (Nice, France) in 2016 and 2017. The performance of pediaTRI to identify high-level emergencies (severity levels 1 and 2) was evaluated in comparison with a PEWS ≥ 4/9. Data from 2018–19 was used as an independent validation cohort.

Results

pediaTRI agreed with the PEWS score for 84,896 of the patients (84.5%): 15.0% (14.8–15.2) of the patients were over-triaged and 0.5% (0.5–0.6) under-triaged compared with the PEWS score. pediaTRI had a sensitivity of 76.4% (74.6–78.2), a specificity of 84.7% (84.4–84.9), and positive and negative likelihood ratios of 5.0 (4.8–5.1) and 0.3 (0.3–0.3), respectively, for the identification of high-level emergencies. However, the positive likelihood ratios were lower for patients presenting with a medical complaint [4.1 (4.0–4.2) v 10.4 (7.9–13.7 for trauma), and for younger children [1.2 (1.1–1.2) from 0 to 28 days, and 1.9 (1.8–2.0) from 28 days to 3 months].

Conclusion

pediaTRI has a moderate to good validity to triage children in a Pediatric Emergency Department with a tendency to over-triage compared with the PEWS system. Its validity is lower for younger children and for children consulting for a medical complaint.
Update on the Coordinated Efforts of Looking After the Health Care Needs of Children and Young People Fleeing the Conflict Zone of Ukraine Presenting to European Emergency Departments—A Joint Statement of the European Society for Emergency Paediatrics and the European Academy of Paediatrics
Authors: Nijman Ruud G. , Bressan Silvia , Brandenberger Julia , Kaur Davi , Keitel Kristina , Maconochie Ian K. , Oostenbrink Rianne , Parri Niccolo , Shavit Itai , Teksam Ozlem , Velasco Roberto , van de Voorde Patrick , Da Dalt Liviana , Guchtenaere Ann De , Hadjipanayis Adamos A. , Ross Russell Robert , del Torso Stefano , Bognar Zsolt , Titomanlio Luigi
Published: April 2022

This joint statement by the European Society for Emergency Paediatrics and European Academy of Paediatrics aims to highlight recommendations for dealing with refugee children and young people fleeing the Ukrainian war when presenting to emergency departments (EDs) across Europe. Children and young people might present, sometimes unaccompanied, with either ongoing complex health needs or illnesses, mental health issues, and injuries related to the war itself and the flight from it. Obstacles to providing urgent and emergency care include lack of clinical guidelines, language barriers, and lack of insight in previous medical history. Children with complex health needs are at high risk for complications and their continued access to specialist healthcare should be prioritized in resettlements programs. Ukraine has one of the lowest vaccination coverages in the Europe, and outbreaks of cholera, measles, diphtheria, poliomyelitis, and COVID-19 should be anticipated. In Ukraine, rates of multidrug resistant tuberculosis are high, making screening for this important. Urgent and emergency care facilities should also prepare for dealing with children with war-related injuries and mental health issues. Ukrainian refugee children and young people should be included in local educational systems and social activities at the earliest opportunity.
Effect of Probiotic Supplements on Oxidative Stress Biomarkers in First-Episode Bipolar Disorder Patients: A Randomized, Placebo-Controlled Trial
Authors: Zeng Cuirong , Qiu Yan , Li Sujuan , Teng Ziwei , Xiang Hui , Chen Jindong , Wu Xiangxin , Cao Ting , Zhang Shuangyang , Chen Qian , Wu Haishan , Cai HuaLin
Published: April 2022

Background: Currently no study has examined the effects of probiotic administration on the symptoms of anxiety, depression, and mania, as well as their correlations with the biomarkers of oxidative stress in patients with bipolar disorder (BPD). The aim of this study is to determine the effects of probiotic supplementation on plasma oxidative stress-related biomarkers and different domains of clinical symptom in patients suffering from BPD.

Methods: Eighty first-episode drug-naive patients with BPD were recruited. The subjects were randomized to receive psychotropic drugs supplementing with either probiotic or placebo and scheduled to evaluate with follow-ups for clinical symptom improvements and changes in the oxidative stress biomarkers. The Hamilton Depression Rating Scale, Hamilton Anxiety Rating Scale, and Young Mania Rating Scale were used to assess the clinical symptomatology. The panel of plasma oxidative stress biomarkers were determined by ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) at baseline and for 3 months of follow-up, i.e., at post-treatment month 1, 2, and 3.

Results: After 3 months of intervention, decreased levels of plasma lysophosphatidylcholines (LPCs) were found in both placebo and probiotic groups. However, six other oxidative stress biomarkers (i.e., creatine, inosine, hypoxanthine, choline, uric acid, allantoic acid) increased in BPD patients after the two types of therapies. In addition, a positive correlation between changes of LPC (18:0) and YMRS scale was found in BPD patients and this association only existed in the probiotic group. Additionally, the mania symptom greatly alleviated (pretreatment–posttreatment, odds ratio = 0.09, 95%CI = 0.01, 0.64, p= 0.016) in patients who received probiotic supplements as compared with the placebo group.

Conclusion: The changes in plasma biomarkers of oxidative stress in patients with BPD have a potential to be trait-like markers, and serve as prognostic indexes for bipolar patients. Daily intakes of probiotics have advantageous effects on BPD patients with certain clinical symptoms, especially manic symptoms. The treatment may be a promising adjunctive therapeutic strategy for BPD patients in manic episode.
Conventional Synthetic Disease-Modifying Anti-rheumatic Drugs for Psoriatic Arthritis: Findings and Implications From a Patient Centered Longitudinal Study in Brazil
Authors: Faria Ronaldo José , Cordeiro Francisca Janiclecia Rezende , dos Santos Jéssica Barreto Ribeiro , Alvares-Teodoro Juliana , Guerra Júnior Augusto Afonso , Acurcio Francisco de Assis , da Silva Michael Ruberson Ribeiro
Published: April 2022

Background: Conventional synthetic disease-modifying antirheumatic drugs are the first-line treatment to inhibit the progression of psoriatic arthritis. Despite their widespread clinical use, few studies have been conducted to compare these drugs for psoriatic arthritis.

Methods: a longitudinal study was carried out based on a centered patient national database in Brazil. Market share of drugs, medication persistence, drug costs, and cost per response were evaluated.

Results: a total of 1,999 individuals with psoriatic arthritis were included. Methotrexate was the most used drug (44.4%), followed by leflunomide (40.6%), ciclosporin (8.2%), and sulfasalazine (6.8%). Methotrexate and leflunomide had a greater market share than ciclosporin and sulfasalazine over years. Medication persistence was higher for leflunomide (58.9 and 28.2%), followed by methotrexate (51.6 and 25.4%) at six and 12 months, respectively. Leflunomide was deemed the most expensive drug, with an average annual cost of $317.25, followed by sulfasalazine ($106.47), ciclosporin ($97.64), and methotrexate ($40.23). Methotrexate was the drug being the lowest cost per response.

Conclusion: Methotrexate had the best cost per response ratio, owing to its lower cost and a slightly lower proportion of persistent patients when compared to leflunomide. Leflunomide had a slightly higher medication persistence than methotrexate, but it was the most expensive drug.
Atomic Insight Into Phase Transition Lowering in Shock Compressed Copper
Authors: Ling Weidong , Chen Bo , Zeng Qiyu , Yu Xiaoxiang , Zhang Shen , Zhao Zengxiu , Dai Jiayu
Published: April 2022

High pressure structural transformation of copper (Cu) is a rather complex physical process. One of the intriguing questions that are rarely discussed is the comparison between quasi-isentropic response and adiabatic response for copper lattice transition. The ambient face-centered-cubic structure of Cu is predicted to persist over 100 TPa from ab inito calculations and experimentally demonstrated to persist until 1.15 TPa in ramp compression and 150 GPa in static compression. However, a novel body-centered-cubic (BCC) order is observed merely at 180 GPa once shock compression is applied. The mechanism of body-centered-cubic phase transition occurred at low pressure under shock compression remains elusive so far and much attention is required on the dynamics in such a phase transition. In this work, we utilize the molecular dynamics method to simulate the shock compression on a copper lattice to uncover the structural transition in the atomic scale. We report the FCC–BCC phase transition occurred at 156 GPa, and lots of disordered structures are discovered in the BCC phase after impact, revealed by a series of structure analysis tools and free energy calculations. The plethora of transient disordered structures reduces the global Gibbs free energies, thus leading to the downgrade of the transition pressure in contrast to the ramp and static compression, which provides a new perspective for structural transformation under extreme conditions.
Effective Dirac Neutrino Mass Operator in the Standard Model With a Local Abelian Extension
Authors: Restrepo Diego , Suarez David
Published: April 2022

We present 48 types of solutions to the anomaly cancellation conditions of local Abelian extensions of the Standard Model (SM) with right-handed SM-singlet chiral fermions. At least two of them acquire effective light Dirac neutrino masses, while the others get heavy masses from the spontaneous symmetry breaking of the local Abelian symmetry, forming a dark sector with multi-component and multi-generational fermionic dark matter. The corresponding effective Dirac neutrino mass operator can be realized at tree-level or radiatively by introducing extra scalars, and in some cases after imposing extra scotogenic conditions. The Dirac Zee model with Dirac fermionic dark matter is presented as an example of model where the neutrino and dark matter phenomenology are basically independent of each other.
No Difference in the Acute Effects of Randomization vs. Blocking of Units of Lower-Extremity Proprioceptive Training on Balance and Postural Control in Young Healthy Male Adults
Authors: Ivusza Patrik , Hortobágyi Tibor , Sebesi Balázs , Gáspár Balázs , Fésüs Ádám , Varga Mátyás , Malmos Vanessza , Váczi Márk
Published: April 2022

Random practice is a form of differential learning and its favorable acute effects on motor performance are well described when visual tasks are practiced. However, no study to date has investigated the acute effects of differential learning using variable proprioceptive stimuli instead of the visual cues. The aim of the present study was to compare the acute effects of randomized versus blocked lower-extremity proprioceptive training stimuli on balance and postural adjustments. In two conditions, healthy young males (n = 15, age = 23 years) performed 16 one-legged landings on a board tilted in four directions: 1) tilt direction unknown and randomized and 2) tilt direction known with order of presentation blocked. Multi-segmental angular sway while balancing on an unstable surface and postural responses to perturbation stimulus by surface tilts were measured before and 4 min after training. Overall frontal-plane postural sway on the unstable surface decreased (p < 0.05, η2 = 0.022) in both conditions, while sagittal-plane postural sway remained unchanged. When the surface was toes-up tilted in the perturbation test, the sagittal-plane shank-thigh-pelvis alignment improved in both conditions (p < 0.05, η2 = 0.017), but the direction of the segmental positioning was non-uniform across participants. We conclude that randomization vs. blocking of units of lower-extremity proprioceptive training did not affect balance and postural control in our cohort of healthy young adults but the improvements were test-specific.
Isolation and Genome Analysis of Pectobacterium colocasium sp. nov. and Pectobacterium aroidearum, Two New Pathogens of Taro
Authors: Zhou Jianuan , Hu Ming , Hu Anqun , Li Chuhao , Ren Xinyue , Tao Min , Xue Yang , Chen Shanshan , Tang Chongzhi , Xu Yiwu , Zhang Lianhui , Zhou Xiaofan
Published: April 2022

Bacterial soft rot is one of the most destructive diseases of taro (Colocasia esculenta) worldwide. In recent years, frequent outbreaks of soft rot disease have seriously affected taro production and became a major constraint to the development of taro planting in China. However, little is known about the causal agents of this disease, and the only reported pathogens are two Dickeya species and P. carotovorum. In this study, we report taro soft rot caused by two novel Pectobacterium strains, LJ1 and LJ2, isolated from taro corms in Ruyuan County, Shaoguan City, Guangdong Province, China. We showed that LJ1 and LJ2 fulfill Koch’s postulates for taro soft rot. The two pathogens can infect taro both individually and simultaneously, and neither synergistic nor antagonistic interaction was observed between the two pathogens. Genome sequencing of the two strains indicated that LJ1 represents a novel species of the genus Pectobacterium, for which the name “Pectobacterium colocasium sp. nov.” is proposed, while LJ2 belongs to Pectobacterium aroidearum. Pan-genome analysis revealed multiple pathogenicity-related differences between LJ1, LJ2, and other Pectobacterium species, including unique virulence factors, variation in the copy number and organization of Type III, IV, and VI secretion systems, and differential production of plant cell wall degrading enzymes. This study identifies two new soft rot Pectobacteriaceae (SRP) pathogens causing taro soft rot in China, reports a new case of co-infection of plant pathogens, and provides valuable resources for further investigation of the pathogenic mechanisms of SRP.
Work Stressors and Occupational Health of Young Employees: The Moderating Role of Work Adaptability
Authors: Zhou Houyu , Zheng Quangquang
Published: April 2022

Work adaptability refers to the work experience, habits, and skills that enable an individual to adapt to current or changing work tasks and situations. It is a coping resource that individuals use to mitigate various types of stress. Adopting the interaction model of work stress, this study investigated 168 young employees in 20 organizations in Zhejiang Province through interview research and a questionnaire survey. The results show that work adaptability has a significant main effect on occupational health. The work adaptability of employees plays a moderating role in the relationship between occupational health and lack of work meaning stress, role conflict stress, interpersonal relationship stress, negative organizational atmosphere stress, and total score of work stressors. Young employees with high work adaptability have worse occupational health under high-level stress situations due to a lack of work meaning. For promoting occupational health in young employees, organizations should have this group of workers complete meaningful jobs or inform them of the importance of their jobs, reduce role conflict, and create a supportive organizational atmosphere. For management, it is imperative to eliminate high-level stress that stems from a lack of work meaning in order to retain young employees with high work adaptability. These findings shed light on how work adaptability helps young employees deal with stress and improve their occupational health. In organizational and self-stress management, it is beneficial to improve employees’ work adaptability continuously as a means of effectively resisting stress and maintaining occupational health.
Effects of Lisdexamfetamine, a Prodrug of D-Amphetamine, on Locomotion, Spatial Cognitive Processing and Neurochemical Profiles in Rats: A Comparison With Immediate-Release Amphetamine
Authors: Jian-min Chen , Zhi-yuan Wang , Shi-xuan Wu , Rui Song , Ning Wu , Jin Li
Published: April 2022

D-amphetamine has been used to enhance cognitive performance over the last few decades. Due to the rapid absorption after administration, d-amphetamine shows narrow effective window and severe abuse potential. Lisdexamfetamine, a prodrug of d-amphetamine, reduces the magnitude of plasma d-amphetamine concentration and prolongs the action duration when compared with immediate-release d-amphetamine at equimolar doses. However, the differences of these two drugs, which produce distinct pharmacokinetic characteristics, in cognition improvement still unclear. In present study, we compared the effects of d-amphetamine (i.p) and lisdexamfetamine (p.o) at equimolar doses (0.2, 0.5, 1.5, 4.5, and 13.5 mg/kg of d-amphetamine base) on locomotion, spatial working memory and recognition memory in rats. Given the crucial involvement of dopamine neurotransmitter system within the medial prefrontal cortex (mPFC) in cognitive processing, microdialysis was conducted to profile the difference in neurochemical characteristics between the two drugs. In our results, d-amphetamine ranges from 0.5 to 1.5 mg/kg significantly increased locomotor activity. However, d-amphetamine ranges from 0.2 to 13.5 mg/kg failed to improve spatial working memory and recognition memory in Y-maze-based spontaneous alternation and two-trial delayed alternation tasks of rats, respectively. In contrast, lisdexamfetamine with 4.5 mg/kg significantly increased the locomotion and improved both spatial working and recognition memory. Further, microdialysis showed that lisdexamfetamine induced lower magnitude and longer duration of extracellular dopamine increase than that of d-amphetamine. These results suggest that lisdexamfetamine was more effective than d-amphetamine in improving spatial cognitive performance, which was attributed to the steady and lasting dopamine release pattern within the mPFC.
Association of Health Literacy With Medication Adherence Mediated by Cognitive Function Among the Community-Based Elders With Chronic Disease in Beijing of China
Authors: Jia Qiaoling , Wang Haiyan , Wang Li , Wang Yanhong
Published: April 2022

Background

Although health literacy was considered to play a crucial role in non-communicable chronic disease (NCD) prevention and control, the relationship of health literacy and medication adherence has rarely given attention among older adult Chinese population in previous studies, especially considered that they might be with cognitive impairment.

Purpose

This study aimed to investigate the association between health literacy and medication adherence and mediation by cognitive ability among community-based older adults with chronic disease in Beijing of China.

Methods

The older adults aged 60 years old or over were recruited in a cross-sectional survey conducted in Beijing of China by using multistage, stratified sampling method. Of those, the participants with chronic disease and need to take long-term medicine were included in our study. The information about sociodemographic characteristics, health literacy, cognition ability, and medication adherence was collected by the questionnaire. The univariate and multiple logistic regression analysis were used to measure the association of health literacy and adherence medication, and mediate effect by cognitive ability.

Results

The total of 4,166 older adult populations (average age: 70.61 ± 7.38 years) was included in this study, 1,395 participants (33.49%) were non-adherence, 1,983 participants (47.60%) had two chronic conditions or more, and 1,459 participants (35.02%) screened as cognitive impairment. The health literacy was negatively associated with medication adherence. The lower total scores of health literacy were found with a high risk of non-adherence [p < 0.01, adjusted odds ratio (OR) = 0.988 per one point increase, 95% CI: 0.982–0.993] controlling other covariates. However, their association tended to be weakened or even disappeared among the older adults with cognitive impairment compared with the populations with normal cognitive.

Conclusion

Improving health literacy might be a public health strategy to increase the medication adherence of older adults, but need to first identify the potential target population based on their cognitive ability.
Effects of Multidimensional Self-Esteems on Health Promotion Behaviors in Adolescents
Authors: Liu Bin , Tian Lu , Yang Shuo , Wang XueQiang , Luo Jiong
Published: April 2022

Objective

To explore the relationships between multidimensional self-esteems and health behaviors among adolescents by demographic background factors, so as to provide an important reference for the intervention of health promotion behavior and self-esteem education in the future.

Methods

Taking adolescents in Chongqing as the object, this paper investigates the students in 24 primary and secondary schools (half of health promotion schools and half of non-promotion schools) by means of Stratified random sampling, and Using SPSS 21.0 and AMOS 19.0 statistical analysis software to process the collected data.

Results

1) gender and age significantly affected adolescents' self-esteem and health promotion behavior, which showed that boys's perception of self-esteem was lower than that of girls, while girls were more likely to implement health promotion behavior than boys; 2) Children from two parent families or families with higher parental education are more able to implement health promotion behavior and enjoy higher self-esteem, while family economic status has no effect on adolescents' self-esteem and health promotion behavior; 3) Compared with students in ordinary schools, adolescents in health promotion schools (HPS) have higher self-esteem and can implement health promotion behavior more; 4) The higher the self-esteem of adolescents, the better their health promotion behavior; The higher the sense of interpersonal ability, the more able to implement social support behavior; The higher the sense of physical ability and physiological value, the more able to implement sports behavior; The higher the sense of academic ability, the more able to implement nutritional behavior; The higher the external recognition and physiological value, the better the performance of nutritional behavior and stress management; The higher the internal evaluation, the more able to implement health responsibility and exercise behavior.

Conclusion

Socio-economic background can indeed have a direct or indirect impact on adolescent health promotion behavior, and multidimensional self-esteem can explain about 70% of the variation of health promotion behavior, which seems to suggest that improving adolescent self-esteem is the focus of health promotion and health education in the future.
Ultrasound-Guided vs. Landmark-Guided Lumbar Puncture for Obese Patients in Emergency Department
Authors: Li Lei , Tao Weichen , Cai Xue
Published: April 2022

Objective

Emergency patients are in severe and urgent condition. If the patient is obese, the traditional lumbar puncture method is more difficult. This study was to observe the comparison of ultrasound-guided and landmark-guided lumbar puncture for obese patients in the emergency department.

Methods

Sixty patients suspected of intracranial infection, subarachnoid hemorrhage, and intraventricular hemorrhage from January 2018 to June 2020 were selected in the Department of Emergency Medicine, Shengjing Hospital of China Medical University. They were randomly assigned to two groups according to the order of enrollment: Group A (Landmark-guided group, n = 30) and Group B (Ultrasound-guided group, n = 30). Follow-up assessments were performed to observe lumbar puncture time, the number of bloody CSF, Visual Analog Scale (VAS), the complications, and satisfaction.

Results

Compared with group A, group B had less lumbar puncture time, lower puncture attempts, and a higher first puncture success rate (P <0.05). In group B, the number of bloody CSF was less (P <0.05), postprocedural low back pain was less (P <0.05), intraprocedural sciatic nerve irritation and postprocedural paresthesia were less, but the difference was not statistically significant (P > 0.05). Compared with group A, the postprocedural VAS in group B was lower, and the difference was statistically significant (P <0.05). The total satisfaction of group A and group B was 60.0 and 86.7%, respectively. The total satisfaction of group B was higher than that of group A (P <0.05).

Discussion

Ultrasound-guided lumbar puncture can be used for obese patients with difficulty in the lumbar puncture. It is worthy of clinical application and promotion.
Vaginal Evisceration of Small Bowel With Extraperitoneal Ileal Resection of the Herniated Loops: A Case Report
Authors: Missori Giulia , Marchesini Nicolò Luigi Arrigo , Mosca Donatella , Ricciardolo Andrea Aurelio , Serra Francesco , Gelmini Roberta
Published: April 2022

Introduction

Vaginal evisceration is an extremely rare surgical emergency that can be described as the extrusion of abdominal viscera through a defect or a rupture of the vaginal wall. We reported the case of an acute abdomen due to small bowel evisceration secondary to vaginal vault dehiscence that required combined vaginal-abdominal approach

Case

We discuss the case of a 72-year-old female who presented to the emergency department for a large prolapse with visible extrusion of the small bowel per vagina. The eviscerated bowel was resected by external vaginal approach due to excessive swelling of the loops which made it impossible to reduce them through the vagina defect. A midline laparotomy was undertaken for further assessment, and the vault defect was closed by transabdominal repair

Conclusion

From its first description in 1864, just a few cases of vaginal evisceration had been described in the medical literature; the most common organ to eviscerate is the distal ileum, although cases of omentum, colon, fallopian tube, and appendix evisceration have also been reported. We described a rare case of transvaginal evisceration of the small bowel in our emergency department; it is a rare surgical emergency that must be managed to prevent serious consequences, such as bowel ischemia and necrosis, sepsis, and death. We suggest that a multidisciplinary approach to prompt examination and management by gynecologists and general surgeons is recommended to reduce the risk of morbidity and mortality. With this paper the authors would like to share the surgical manage of this rare emergency with other surgeons all around the world.
Breast Metastasis of Lung Cancer After Computed Tomography-Guided Core Needle Biopsy: A Case Report
Authors: Zhao Rong , Xing Jun , Gao Jinnan
Published: April 2022

Background

Needle tract metastasis is a rare complication following percutaneous procedures for malignancy.

Case Summary

This report describes a 49-year-old female with a lump on her right breast. Mass core needle biopsy showed the specimen was an invasive carcinoma, and mastectomy with sentinel lymph node biopsy was performed. What is special about this case was that the patient reported a history of lung cancer and the position of the breast mass was the puncture site of computed tomography-guided core needle biopsy for lung cancer. Immunostaining of paraffin specimen findings indicated the breast mass as a result of lung carcinoma metastasis. The patient's medical history indicated that the malignant tumor in the breast was a core needle tract pulmonary metastasis. The patient underwent the examination and received therapy based on the lung cancer metastasis principle. At 9 months from breast surgery, the patient is alive, in good condition, and with stability of the disease.

Conclusions

This patient was misdiagnosed. Careful medical history review and multidisciplinary team discussions are important, especially for patients with a history of cancer or invasive operation.
Comparison of Fluid Analysis and Cytologic Findings of Cerebrospinal Fluid Between Three Collection Sites in Adult Equids With Neurological Disease
Authors: Young Kimberly A. S. , Hepworth-Warren Kate L. , Dembek Katarzyna A.
Published: April 2022

Cerebrospinal fluid (CSF) is routinely collected from three sites in the horse, the atlanto-occipital (AO), atlantoaxial (AA), and lumbosacral (LS) space. A comparison between fluid analysis parameters [total protein, total nucleated cell count (TNCC), red blood cell (RBC) count, and morphologic analysis] from samples obtained at each of the three sites has not previously been performed. A retrospective analysis was performed to evaluate the differences in fluid analysis of CSF between the AO, AA, and LS sites in equids presented to a referral service for evaluation of suspected neurological disease. A total of 113 equids aged ≥1 year that underwent CSF collection between 2008 and 2020 were included. Total nucleated cell count, RBC concentration, total protein (TP), and morphologic evaluation between CSF samples obtained from the three sites were compared. When comparing all samples, LS centesis was associated with higher RBC compared to other sites (p< 0.05); TP was lower in the AA group than in the LS group (p< 0.05). Within a subset of cytologically unremarkable samples, RBC concentration was highest in LS samples (p< 0.01); TP was higher in LS samples compared to AA samples (p< 0.05) and TNCC was higher (p< 0.01) in AA and LS groups compared to the AO. In cytologically abnormal samples, there were no significant differences between sites in any parameter. Abnormal cytology was correlated with non-survival (p= 0.0002). Non-survival was associated with higher TNCC (p< 0.01). The receiver operating characteristic (ROC) curve for TNCC had an area under the curve of 0.67 (95% CI, 0.55–0.79) and indicated that a cutoff value of 24 cells/μL maximized specificity (72%) and sensitivity (54%) to predict non-survival in all horses. Positive predictive value was 45%; negative predictive value was 78%. The concentration of RBC was higher in samples from the LS site. This has clinical implications due to the importance of comparative diagnostics and its potential impact on cytologic evaluation. There were minimal differences in multiple other parameters between sites, which are likely clinically insignificant.
The Common Mosquito (Culex pipiens) Does Not Seem to Be a Competent Vector for Hepatitis E Virus Genotype 3
Authors: Frías Mario , Casades-Martí Laia , Risalde María Á. , López-López Pedro , Cuadrado-Matías Raúl , Rivero-Juárez Antonio , Rivero Antonio , Ruiz-Fons Francisco
Published: April 2022

An experimental infection approach was used to estimate the competence of the common mosquito, Culex pipiens, for hepatitis E virus replication and transmission, using an isolate of hepatitis E virus genotype 3 of human origin in varying infectious doses. The experimental approach was carried out in biosafety level 2 conditions on three batches of 120 Cx. pipiens females, each using an artificial feeding system containing the virus in aliquots of fresh avian blood. Mosquitoes from each batch were collected 1, 7, 14, and 21 days post-infection (dpi) and dissected. The proboscis was subjected to forced excretion of saliva to estimate potential virus transmission. HEV RNA presence in abdomen, thorax, and saliva samples was analyzed by PCR at the selected post-infection times. HEV RNA was detected in the abdomens of Cx. pipiens females collected 1 dpi in the two experimentally-infected batches, but not in the saliva or thorax. None of the samples collected 7–21 dpi were positive. Our results show that Cx. pipiens is not a competent vector for HEV, at least for zoonotic genotype 3.
Role of Animal Models to Advance Research of Bacterial Osteomyelitis
Authors: Billings Caroline , Anderson David E.
Published: April 2022

Osteomyelitis is an inflammatory bone disease typically caused by infectious microorganisms, often bacteria, which causes progressive bone destruction and loss. The most common bacteria associated with chronic osteomyelitis is Staphylococcus aureus. The incidence of osteomyelitis in the United States is estimated to be upwards of 50,000 cases annually and places a significant burden upon the healthcare system. There are three general categories of osteomyelitis: hematogenous; secondary to spread from a contiguous focus of infection, often from trauma or implanted medical devices and materials; and secondary to vascular disease, often a result of diabetic foot ulcers. Independent of the route of infection, osteomyelitis is often challenging to diagnose and treat, and the effect on the patient's quality of life is significant. Therapy for osteomyelitis varies based on category and clinical variables in each case. Therapeutic strategies are typically reliant upon protracted antimicrobial therapy and surgical interventions. Therapy is most successful when intensive and initiated early, although infection may recur months to years later. Also, treatment is accompanied by risks such as systemic toxicity, selection for antimicrobial drug resistance from prolonged antimicrobial use, and loss of form or function of the affected area due to radical surgical debridement or implant removal. The challenges of diagnosis and successful treatment, as well as the negative impacts on patient's quality of life, exemplify the need for improved strategies to combat bacterial osteomyelitis. There are many in vitro and in vivo investigations aimed toward better understanding of the pathophysiology of bacterial osteomyelitis, as well as improved diagnostic and therapeutic strategies. Here, we review the role of animal models utilized for the study of bacterial osteomyelitis and their critically important role in understanding and improving the management of bacterial osteomyelitis.