bluuebunny/crossref_metadata_2025 huggingface dataset:
Government policy performance and central–local political trust in China
Authors: Zhai Yida
Published: August 2022

It is widely acknowledged that poor government policy performance undermines public trust in government. However, there has been insufficient study of how citizens attribute responsibility to different levels of government within an authoritarian unitary context. Inquiry is needed to assess the ways government performance in various policy domains affects central–local political trust. This study uses the case of China to addresses these questions. Results show that, in particular, local governments risk losing public trust for corruption. Nevertheless, the central government loses public trust due to unsatisfying economies. Both local and central governments lose public trust for poor performance regarding environmental protection, food safety problems, public health, and primary/middle school education. The central government cannot always avoid blame, depending on different policy issues. The results indicate that the perceived administrative responsibility of different levels of government affects citizens’ evaluations of the performance of the central and local governments as well as their political trust.
Breeding and global population sizes of the Critically Endangered Red-fronted MacawAra rubrogenysrevisited
Authors: Herzog Sebastian K. , Boorsma Tjalle , Saldaña-Covarrubias Guido , Calahuma-Arispe Tomás , Camacho-Reyes Teodoro , Dekker Dirk , Edwards de Vargas Suzanne , García-Cárdenas Máximo , García-Solíz Víctor Hugo , Quiroz-Calizaya Jazmín M. , Quispe-Solíz de Dekker Sayda , Salvatierra-Gómez Marcia M. , Vargas Ramón , Soria-Auza Rodrigo W.
Published: August 2022

The ‘Critically Endangered’ Red-fronted Macaw is endemic to seasonally dry, rain-shadowed valleys in the south-central Andes of Bolivia. The remoteness and inaccessibility of most of this region have hampered the rigorous collection of reliable range-wide data on the species’ global, local and breeding population sizes. Such data are imperative, however, for effective conservation and management. Estimated to number up to 5,000 birds in the early 1980s, the most recent and thorough survey to date reported a total of only 807 macaws and a breeding population fraction of about 20% in 2011, disjunctly distributed across eight breeding and six foraging areas and divided into four genetic clusters. Ten years later, we reassessed the species’ population sizes and breeding distribution with increased survey effort and geographic coverage. Six teams simultaneously surveyed different sections of the species’ entire known breeding range in four watersheds focusing on nesting sites. We estimated a global population size of 1,160 macaws, a breeding population fraction of 23.8–27.4% (138–159 nesting pairs) and discovered four new breeding areas. Watersheds and breeding areas differed widely in nesting pair and total macaw numbers. The Mizque watershed held 53% of the species’ breeding and 41.5% of its global population and had the highest breeding population fraction of 30.7–34.9%; the Pilcomayo watershed obtained the lowest values (6%, 8.5% and 14.1–18.2%, respectively). Two of the four documented genetic clusters (subpopulations) each held well over 50 breeding individuals. Two of the eight breeding areas documented in 2011 were found unoccupied in 2021. Numbers of nesting pairs per breeding area in 2011 were poorly correlated with those in 2021, and timing of breeding activities also differed between years. Our new data indicate that the Red-fronted Macaw no longer meets IUCN Red List criteria for ‘Critically Endangered’ species and that it should be downlisted to ‘Endangered.’
Acute vasodilator response testing in the adult Fontan circulation using non-invasive 4D Flow MRI: a proof-of-principle study
Authors: McConnell Benjamin , Stoll Victoria M. , Panayiotou Hannah , Piechnik Stefan K. , Neubauer Stefan , van der Geest Rob J. , Myerson Saul G. , Orchard Elizabeth , Bissell Malenka M.
Published: August 2022

Background:

Pulmonary vasodilator therapy in Fontan patients can improve exercise tolerance. We aimed to assess the potential for non-invasive testing of acute vasodilator response using four-dimensional (D) flow MRI during oxygen inhalation.

Materials and Methods:

Six patients with well-functioning Fontan circulations were prospectively recruited and underwent cardiac MRI. Ventricular anatomical imaging and 4D Flow MRI were acquired at baseline and during inhalation of oxygen. Data were compared with six age-matched healthy volunteers with 4D Flow MRI scans acquired at baseline.

Results:

All six patients tolerated the MRI scan well. The dominant ventricle had a left ventricular morphology in all cases. On 4D Flow MRI assessment, two patients (Patients 2 and 6) showed improved cardiac filling with improved preload during oxygen administration, increased mitral inflow, increased maximum E-wave kinetic energy, and decreased systolic peak kinetic energy. Patient 1 showed improved preload only. Patient 5 showed no change, and patient 3 had equivocal results. Patient 4, however, showed a decrease in preload and cardiac filling/function with oxygen.

Discussion:

Using oxygen as a pulmonary vasodilator to assess increased pulmonary venous return as a marker for positive acute vasodilator response would provide pre-treatment assessment in a more physiological state – the awake patient. This proof-of-concept study showed that it is well tolerated and has shown changes in some stable patients with a Fontan circulation.
Gender roles, generational changes and environmental challenges: an intersectional interpretation of perceptions on healthy diets among iTaukei women and men in Fiji
Authors: McKenzie Briar Louise , Waqa Gade , Hart Ashleigh Chanel , Moala Silatolu Anasaini , Palagyi Anna , Norton Robyn , McLean Rachael , Webster Jacqui
Published: August 2022

Objective:

To investigate perceptions of iTaukei Fijian women and men around diet and the ability to consume a healthy diet.

Design:

Six focus groups were conducted with women and men separately. Six to ten women and men participated in each group. Discussions were recorded, transcribed, translated and thematically analysed. Themes were mapped to an intersectionality framework to aid interpretation.

Setting:

Four villages in Viti Levu, Fiji.

Participants:

Twenty-two women and twenty-four men.

Results:

Seven overarching themes were identified, including generational changes in food behaviour, strong-gendered beliefs around food and food provision, cultural and religious obligations around food, the impact of environmental change on the ability to consume a healthy diet, perceptions of the importance of food, food preferences and knowledge. Participants across focus groups identified that it was the ‘duty’ of women to prepare food for their families. However, some women reflected on this responsibility being unbalanced with many women now in the formal workforce. Changes between generations in food preferences and practices were highlighted, with a perception that previous generations were healthier. Power dynamics and external factors, such as environmental changes, were identified by women and men as crucial influences on their ability to eat a healthy diet.

Conclusion:

Embedded traditional perceptions of gendered roles related to nutrition were misaligned with other societal and environmental changes. Given factors other than gender, such as broader power dynamics and environmental factors were identified as influencing diet, viewing nutrition-related issues through an intersectional lens is important to inform equitable food policy in Fiji.
The benefits of concurrent engagement in meaning-centered psychotherapy and meaning-centered psychotherapy for cancer caregivers: A case study
Authors: Roberts Kailey E. , Applebaum Allison J.
Published: August 2022

Objective

This report describes a case of concurrent engagement in Meaning-Centered Psychotherapy (MCP) and Meaning-Centered Psychotherapy for Caregivers (MCP-C), brief, structured interventions designed to address existential distress in patients with cancer and cancer caregivers.

Method

Descriptions of the independent courses of MCP/MCP-C treatment for a patient with Glioblastoma Multiforme and his caregiver are provided with both unique and shared themes around sources of meaning highlighted.

Results

The patient and caregiver each experienced enhanced well-being as a result of receiving MCP and MCP-C, as well as shared benefits of deepened connectedness. Engagement in MCP/MCP-C had important implications for their experience of the patient's end-of-life and the caregiver's bereavement.

Significance of results

MCP and MCP-C are interventions typically delivered independently to patients and caregivers. The individual and shared benefits derived from MCP/MCP-C by this patient and caregiver point to the potential benefits of concurrent engagement and the need for future dyadic research on MCP/MCP-C.
Taking Voice Seriously
Authors: Schoppa Leonard J.
Published: August 2022

Fifty years after the publication ofExit, Voice, and Loyalty, Albert Hirschman’s work is receiving renewed attention in leading journals, including efforts to develop formal models and new strands of democratic theory that capture his insights. These efforts have led some to conclude that power flows exclusively from an ability to exit at low cost, and that we can only empower the weaker elements in society by giving them more choices—more ability to switch from one public goods supplier to another. I examine work by leading proponents of these views and argue that their approaches ignore the role that constrained exit opportunities play in fomenting collective action and voice, not merely to signal dissatisfaction, but to provide information on where leaders have gone too far and what needs to be done. We should expect to see leaders respond to member dissatisfaction both when members can cheaply leaveandwhen they have no way out, with the problematic zone lying in between—exactly where “voucher” systems for public goods propose to take us.
Seismic Imaging of Dante's Domes Oceanic Core Complex From Streamer Waveform Inversion and Reverse Time Migration
Authors: Zhang Maochuan , Di Huizhe , Xu Min , Canales Juan Pablo , Yu Chuanhai , Zhao Xu , Wang Peifeng , Zeng Xin , Wang Yue
Published: August 2022

Early arrival traveltime tomography and full waveform inversion were conducted on downward continued streamer seismic data at Dante's Domes oceanic core complex (OCC), providing unprecedented details of shallow P wave velocity structure. Together with reverse time migration images, seafloor morphology, in situ geological samples, magnetic and gravity data, the seismic constraints are used to infer the lithological distribution along the seismic profiles. Based on the striking similarity in velocity structure beneath the corrugated domes with other OCCs and drilling results from Atlantis Massif, we confidently reconfirmed the Southern Dome as dominantly gabbroic rocks, and the Northern Dome as serpentinized peridotites. A series of isolated gabbroic bodies embedded in the diabase and basaltic layers is observed in the breakaway zone, suggesting that the initiation of Dante's Domes OCC occurred over a long period during which there were several failed attempts to form a long‐lived detachment fault. This early development of the OCC probably occurred under a regime of alternating magma starvation and magma replenishment. The predominantly gabbroic section, beneath the Southern Dome and extending to termination, indicates the OCC has been created with relatively high magma flux. We also imaged distinct shallow subseafloor reflections which are also termed as D reflectors underneath the corrugated domes. The location of the D reflectors is similar to those in the Atlantis Massif, with depths well correlated with the top of exhumed gabbroic bodies and the discontinuities in the D reflectors between gabbroic bodies. Our findings contribute to the understanding of processes controlling the OCCs initiation and evolution at slow spreading ridges.
Watershed‐Geochemical Model to Simulate Dissolved and Particulate 137Cs Discharge From a Forested Catchment
Authors: Sakuma Kazuyuki , Hayashi Seiji , Yoshimura Kazuya , Kurikami Hiroshi , Malins Alex , Funaki Hironori , Tsuji Hideki , Kobayashi Takamaru , Kitamura Akihiro , Iijima Kazuki
Published: August 2022

Measurements in forest catchments in Fukushima Prefecture indicate that cesium‐137 (137Cs) leaching from forest litter affects dissolved 137Cs levels in river water. Existing simulations have been unsuccessful in reproducing this phenomenon due to mechanistic limitations in the models connecting the forest ecosystem and the river system. This paper introduces a new coupled watershed‐geochemical model to address this. We connected a forest ecosystem compartment model to the 3D watershed model General‐purpose Terrestrial Fluid‐Flow Simulator to describe 137Cs transfer between forests and the river system. The compartment model included nonleachable and leachable 137Cs sites in the organic layer. The latter sites model how leachable 137Cs stocks increase with ambient temperature owing to decomposing organic matter. The model was tested by simulating dissolved and particulate 137Cs discharges from a forested catchment upstream of the Ohta River, Fukushima Prefecture. The results for dissolved 137Cs concentrations in river water for 1 January 2014, to 31 December 2015, correlated well with catchment measurements. The simulations reproduced dissolved 137Cs concentration peaks that occurred during three typhoon events and the seasonal variations under baseflow conditions. The results support the theory that leaching from the organic layer in forests is a primary factor affecting river water dissolved 137Cs concentrations. The wider contribution of this study is it introduces a coupled watershed‐geochemical model, applicable not only for evaluating the dynamics of 137Cs, but also those of other substances that adsorb onto sediments and soil particles, and leach from forest organic matter.
Science: Through the Years
Authors: Borovsky Joseph E.
Published: August 2022

In a perspective fashion, viewpoints on three research topics are presented, advice is given for early researchers and for not‐so‐early researchers, comments are made about the impacts of luck and hard work on a science career, and a lifetime of colleagues is discussed.
Phospholipase D2 controls bone homeostasis by modulating M-CSF-dependent osteoclastic cell migration and microtubule stability
Authors: Kim Hyun-Ju , Lee Dong-Kyo , Jin Xian , Che Xiangguo , Ryu Sung Ho , Choi Je-Yong
Published: August 2022

Phospholipase D2 (PLD2), a signaling protein, plays a central role in cellular communication and various biological processes. Here, we show that PLD2 contributes to bone homeostasis by regulating bone resorption through osteoclastic cell migration and microtubule-dependent cytoskeletal organization. Pld2-deficient mice exhibited a low bone mass attributed to increased osteoclast function without altered osteoblast activity. While Pld2 deficiency did not affect osteoclast differentiation, its absence promoted the migration of osteoclast lineage cells through a mechanism involving M-CSF-induced activation of the PI3K–Akt–GSK3β signaling pathway. The absence of Pld2 also boosted osteoclast spreading and actin ring formation, resulting in elevated bone resorption. Furthermore, Pld2 deletion increased microtubule acetylation and stability, which were later restored by treatment with a specific inhibitor of Akt, an essential molecule for microtubule stabilization and osteoclast bone resorption activity. Interestingly, PLD2 interacted with the M-CSF receptor (c-Fms) and PI3K, and the association between PLD2 and c-Fms was reduced in response to M-CSF. Altogether, our findings indicate that PLD2 regulates bone homeostasis by modulating osteoclastic cell migration and microtubule stability via the M-CSF-dependent PI3K–Akt–GSK3β axis.
Heart failure in obesity: insights from proteomics in patients treated with or without weight-loss surgery
Authors: Karason Kristjan , Girerd Nicolas , Andersson-Asssarsson Johanna , Duarte Kevin , Taube Magdalena , Svensson Per-Arne , Huby Anne-Cecile , Peltonen Markku , Carlsson Lena M. , Zannad Faiez
Published: August 2022

Background

Obesity is associated with incident heart failure (HF), but the underlying mechanisms are unclear.

Methods

We performed a nested case-control study within the Swedish-Obese-Subjects study, by identifying 411 cases who developed HF and matched them with respect to age, sex, weight-loss-surgery and length of follow-up with 410 controls who did not develop HF. In analyses corrected for multiple testing, we studied 182 plasma proteins known to be related to cardiovascular disease to investigate whether they could add to the understanding of the processes underlying obesity-related HF.

Results

A total of 821 subjects were followed for 16 ± 6 years. Multivariable analysis adjusted for matching variables revealed that 32 proteins were significantly associated with HF. Twelve proteins were related to HF ≥ 80% of the time using a bootstrap resampling approach (false-discovery-rate [FDR] < 0.05): 11 were associated with increased HF-risk: TNFRSF10A*, ST6GAL1, PRCP, MMP12, TIMP1, CCL3, QPCT, ANG, C1QTNF1, SERPINA5 and GAL-9; and one was related to reduced HF-risk: LPL. An further 20 proteins were associated with onset of HF 50–80% of the time using bootstrap resampling (FDR < 0.05). A pathway analysis including all significant 32 proteins suggested that these biomarkers were related to inflammation, matrix remodeling, cardiometabolic hormones and hemostasis. Three proteins, C1QTNF1, FGF-21 and CST3, reflecting dyslipidemia and kidney disease, displayed a higher association with HF in patients who did not undergo weight-loss-surgery and maintained with obesity.

Conclusion

Pathways associated with HF in obesity include inflammation, matrix remodeling, cardiometabolic hormones and hemostasis; three protein biomarkers predicting HF appeared to be obesity-specific.
Regulation of social hierarchy learning by serotonin transporter availability
Authors: Janet Remi , Ligneul Romain , Losecaat-Vermeer Annabel B. , Philippe Remi , Bellucci Gabriele , Derrington Edmund , Park Soyoung Q. , Dreher Jean-Claude
Published: August 2022

Learning one’s status in a group is a fundamental process in building social hierarchies. Although animal studies suggest that serotonin (5-HT) signaling modulates learning social hierarchies, direct evidence in humans is lacking. Here we determined the relationship between serotonin transporter (SERT) availability and brain systems engaged in learning social ranks combining computational approaches with simultaneous PET-fMRI acquisition in healthy males. We also investigated the link between SERT availability and brain activity in a non-social control condition involving learning the payoffs of slot machines. Learning social ranks was modulated by the dorsal raphe nucleus (DRN) 5-HT function. BOLD ventral striatal response, tracking the rank of opponents, decreased with DRN SERT levels. Moreover, this link was specific to the social learning task. These findings demonstrate that 5-HT plays an influence on the computations required to learn social ranks.
Circular RNA circNFKB1 promotes osteoarthritis progression through interacting with ENO1 and sustaining NF-κB signaling
Authors: Tang Su’an , Nie Xiaoyu , Ruan Jianzhao , Cao Yumei , Kang Jingliang , Ding Changhai
Published: August 2022

Inflammatory cytokines-induced activation of the nuclear factor κB (NF-κB) pathway plays a critical role in the pathogenesis of osteoarthritis (OA). Circular RNA (circRNA) has been identified as important epigenetic factor in numerous diseases. However, the biological roles of inflammation-related circRNAs in regulating OA pathogenesis remain elusive. Here, we revealed circRNA expression profiles in human primary chondrocytes with interleukin-1β (IL-1β) stimulation by circRNA sequencing. We identified a highly upregulated circRNA, termed as circNFKB1 in inflamed chondrocytes and osteoarthritic cartilage. As a circRNA derived from exon 2–5 of NFKB1, circNFKB1 is located in both cytoplasm and nucleus of chondrocytes. Furthermore, knockdown of circNFKB1 inhibited extracellular matrix (ECM) catabolism and rescued IL-1β impaired ECM anabolism whereas ectopic expression of circNFKB1 significantly promoted chondrocytes degradation in vitro. Moreover, intraarticular injection of adenovirus-circNFKB1 in mouse joints triggered spontaneous cartilage loss and OA development. Mechanistically, circNFKB1 interacted with α-enolase (ENO1), regulated the expression of its parental geneNFKB1and sustained the activation of NF-κB signaling pathway in chondrocytes. Therefore, this study highlights a novel ENO1-interacting circNFKB1 in OA pathogenesis, and provides valuable insights into understanding the regulatory mechanism of NF-κB signaling in chondrocytes and a promising therapeutic target for the treatment of OA.
GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation
Authors: Wei Shupei , Zhao Qin , Zheng Ke , Liu Peiying , Sha Nannan , Li Yingzi , Ma Chunmin , Li Jingjie , Zhuo Lingang , Liu Guanxin , Liang Wenhua , Jiang Yuhui , Chen Tao , Zhong Nanshan
Published: August 2022

Reprogrammed cell metabolism is deemed as one of the hallmarks of cancer. Hexosamine biosynthesis pathway (HBP) acts as an “energy sensor” in cells to regulate metabolic fluxes. Glutamine-fructose-6-phosphate amidotransferase 1 (GFAT1), the rate-limiting enzyme of HBP, is broadly found with elevated expression in human cancers though its exact and concrete role in tumorigenesis still remains unknown and needs further investigation. P38 mitogen-activated protein kinase (MAPK) is an important component of stress-signaling pathway and plays a critical role in cell fate decision, whereas the underlying mechanism of its activation under nutrient stress also remains elusive. In this study, we show that glucose deprivation induces the interaction of GFAT1 with transforming growth factor β-activated kinase 1 binding protein 1 (TAB1) in a TAB1 S438 phosphorylation-dependent manner. Subsequently, the binding of GFAT1 to TAB1 facilitates TTLL5–GFAT1–TAB1 complex formation, and the metabolic activity of GFAT1 for glutamate production further contributes to TTLL5-mediated TAB1 glutamylation. In consequence, TAB1 glutamylation promotes the recruitment of p38α MAPK and thus drives p38 MAPK activation. Physiologically, GFAT1-TAB1-p38 signaling promotes autophagy occurrence and thus protects tumor cell survival under glucose deficiency. Clinical analysis indicates that both GFAT1 and TAB1 S438 phosphorylation levels correlate with the poor prognosis of lung adenocarcinoma patients. These findings altogether uncover an unidentified mechanism underlying p38 MAPK signaling regulation by metabolic enzyme upon nutrient stress and provide theoretical rationality of targeting GFAT1 for cancer treatment.
Hemoglobinopathy screening in primary care in the Netherlands: exploring the problems and needs of patients and general practitioners
Authors: van Vliet Margo E. , Kerkhoffs Jean-Louis H. , Harteveld Cornelis L. , Houwink Elisa. J. F.
Published: August 2022

The prevalence of hemoglobinopathies in The Netherlands is increasing due to migration. Hemoglobinopathies are severe hereditary diseases. An informed reproductive choice by at-risk couples, such as pre-implantation diagnosis or termination of affected pregnancies, can be made if carriers are detected prior to conception. Using a qualitative design, the needs and wishes of patients, carriers and general practitioners were evaluated regarding carrier detection of hemoglobinopathies in primary care practice. 30 semi-structured interviews were established with 10 general practitioners, 10 patients and 10 carriers. The interviews were audio-recorded, transcribed verbatim and analysed using content analysis to identify recurring themes. Three themes were generated regarding carrier detection of hemoglobinopathies: (1) a need for more information about hemoglobinopathy, (2) a need for indications when to refer for analysis (carrier diagnostics) and (3) insight concerning organization and roles in care for hemoglobinopathy carriers and patients. These themes reflected a need to increase awareness of hemoglobinopathy, improve competences among general practitioners through better education and improvement of communication with patients and their unidentified family members. This study shows the scope of the problem and the critical need for action to improve informed reproductive decision making for the at-risk population.
Unexpected diagnosis of myotonic dystrophy type 2 repeat expansion by genome sequencing
Authors: Rafehi Haloom , Green Cherie , Bozaoglu Kiymet , Gillies Greta , Delatycki Martin B. , Lockhart Paul J. , Scheffer Ingrid E. , Bahlo Melanie
Published: August 2022

Several neurological disorders, such as myotonic dystrophy are caused by expansions of short tandem repeats (STRs) which can be difficult to detect by molecular tools. Methodological advances have made repeat expansion (RE) detection with whole genome sequencing (WGS) feasible. We recruited a multi-generational family (family A) ascertained for genetic studies of autism spectrum disorder. WGS was performed on seven children from four nuclear families from family A and analyzed for REs of STRs known to cause neurological disorders. We detected an expansion of a heterozygous intronic CCTG STR in CNBP in two siblings. This STR causes myotonic dystrophy type 2 (DM2). The expansion did not segregate with the ASD phenotype. Repeat-primed PCR showed that the DM2 CCTG motif was expanded above the pathogenic threshold in both children and their mother. On subsequent examination, the mother had mild features of DM2. We show that screening of STRs in WGS datasets has diagnostic utility, both in the clinical and research domain, with potential management and genetic counseling implications.
A strategy to assess spillover risk of bat SARS-related coronaviruses in Southeast Asia
Authors: Sánchez Cecilia A. , Li Hongying , Phelps Kendra L. , Zambrana-Torrelio Carlos , Wang Lin-Fa , Zhou Peng , Shi Zheng-Li , Olival Kevin J. , Daszak Peter
Published: August 2022

Emerging diseases caused by coronaviruses of likely bat origin (e.g., SARS, MERS, SADS, COVID-19) have disrupted global health and economies for two decades. Evidence suggests that some bat SARS-related coronaviruses (SARSr-CoVs) could infect people directly, and that their spillover is more frequent than previously recognized. Each zoonotic spillover of a novel virus represents an opportunity for evolutionary adaptation and further spread; therefore, quantifying the extent of this spillover may help target prevention programs. We derive current range distributions for known bat SARSr-CoV hosts and quantify their overlap with human populations. We then use probabilistic risk assessment and data on human-bat contact, human viral seroprevalence, and antibody duration to estimate that a median of 66,280 people (95% CI: 65,351–67,131) are infected with SARSr-CoVs annually in Southeast Asia. These data on the geography and scale of spillover can be used to target surveillance and prevention programs for potential future bat-CoV emergence.
Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice
Authors: Roa Juan , Ruiz-Cruz Miguel , Ruiz-Pino Francisco , Onieva Rocio , Vazquez Maria J. , Sanchez-Tapia Maria J. , Ruiz-Rodriguez Jose M. , Sobrino Veronica , Barroso Alexia , Heras Violeta , Velasco Inmaculada , Perdices-Lopez Cecilia , Ohlsson Claes , Avendaño Maria Soledad , Prevot Vincent , Poutanen Matti , Pinilla Leonor , Gaytan Francisco , Tena-Sempere Manuel
Published: August 2022

Kiss1 neurons, producing kisspeptins, are essential for puberty and fertility, but their molecular regulatory mechanisms remain unfolded. Here, we report that congenital ablation of the microRNA-synthesizing enzyme, Dicer, in Kiss1 cells, causes late-onset hypogonadotropic hypogonadism in both sexes, but is compatible with pubertal initiation and preserved Kiss1 neuronal populations at the infantile/juvenile period. Yet, failure to complete puberty and attain fertility is observed only in females. Kiss1-specific ablation of Dicer evokes disparate changes of Kiss1-cell numbers and Kiss1/kisspeptin expression between hypothalamic subpopulations during the pubertal-transition, with a predominant decline in arcuate-nucleus Kiss1 levels, linked to enhanced expression of its repressors, Mkrn3, Cbx7 and Eap1. Our data unveil that miRNA-biosynthesis in Kiss1 neurons is essential for pubertal completion and fertility, especially in females, but dispensable for initial reproductive maturation and neuronal survival in both sexes. Our results disclose a predominant miRNA-mediated inhibitory program of repressive signals that is key for precise regulation of Kiss1 expression and, thereby, reproductive function.
Protection of Omicron sub-lineage infection against reinfection with another Omicron sub-lineage
Authors: Chemaitelly Hiam , Ayoub Houssein H. , Coyle Peter , Tang Patrick , Yassine Hadi M. , Al-Khatib Hebah A. , Smatti Maria K. , Hasan Mohammad R. , Al-Kanaani Zaina , Al-Kuwari Einas , Jeremijenko Andrew , Kaleeckal Anvar Hassan , Latif Ali Nizar , Shaik Riyazuddin Mohammad , Abdul-Rahim Hanan F. , Nasrallah Gheyath K. , Al-Kuwari Mohamed Ghaith , Butt Adeel A. , Al-Romaihi Hamad Eid , Al-Thani Mohamed H. , Al-Khal Abdullatif , Bertollini Roberto , Abu-Raddad Laith J.
Published: August 2022

There is significant genetic distance between SARS-CoV-2 Omicron (B.1.1.529) variant BA.1 and BA.2 sub-lineages. This study investigates immune protection of infection with one sub-lineage against reinfection with the other sub-lineage in Qatar during a large BA.1 and BA.2 Omicron wave, from December 19, 2021 to March 21, 2022. Two national matched, retrospective cohort studies are conducted to estimate effectiveness of BA.1 infection against reinfection with BA.2 (N = 20,994; BA.1-against-BA.2 study), and effectiveness of BA.2 infection against reinfection with BA.1 (N = 110,315; BA.2-against-BA.1 study). Associations are estimated using Cox proportional-hazards regression models after multiple imputation to assign a sub-lineage status for cases with no sub-lineage status (using probabilities based on the test date). Effectiveness of BA.1 infection against reinfection with BA.2 is estimated at 94.2% (95% CI: 89.2–96.9%). Effectiveness of BA.2 infection against reinfection with BA.1 is estimated at 80.9% (95% CI: 73.1–86.4%). Infection with the BA.1 sub-lineage appears to induce strong, but not full immune protection against reinfection with the BA.2 sub-lineage, and vice versa, for at least several weeks after the initial infection.
Temporal evolution of the Mediterranean fin whale song
Authors: Best Paul , Marxer Ricard , Paris Sébastien , Glotin Hervé
Published: August 2022

We present an analysis of fin whale (Balaenoptera physalus) songs on passive acoustic recordings from the Pelagos Sanctuary (Western Mediterranean Basin). The recordings were gathered between 2008 and 2018 using 2 different hydrophone stations. We show how 20 Hz fin whale pulses can be automatically detected using a low complexity convolutional neural network (CNN) despite data variability (different recording devices exposed to diverse noises). The pulses were further classified into the two categories described in past studies and inter pulse intervals (IPI) were measured. The results confirm previous observations on the local relationship between pulse type and IPI with substantially more data. Furthermore we show inter-annual shifts in IPI and an intra-annual trend in pulse center frequency. This study provides new elements of comparison for the understanding of long term fin whale song trends worldwide.
Analysis of related factors between the occurrence of secondary epidermoid cyst of penis and circumcision
Authors: Yu JiangFan , Tang Rui , Ding Ke
Published: August 2022

Secondary epidermoid cyst of the penis is a very rare epidermoid cyst that occurs in the penis. The purpose of this study was to investigate the relationship between the occurrence of secondary epidermoid cyst of penis and circumcision-related factors, and to provide possible reasonable and effective suggestions for circumcision. The data of all patients who visited the clinic for epidermoid cysts of the penis from September 2000 to September 2021 in Xiangya Hospital were collected. A retrospective study was carried out on whether the patients had been circumcised and the surgical method, anesthesia method, cyst location, surgical age, postoperative wound infection, whether they were phimosis patients, and the level of the surgeon. Among the 24 patients followed up, 95.8% had a history of circumcision, and only 4.2% had no history of circumcision, and the more traumatic surgical methods developed secondary epidermoid cyst of the penis after surgery the higher the probability. Injecting anesthesia at the base of the penis increases the chances of developing a secondary epidermoid cyst of the penis. Postoperative secondary epidermoid cyst of the penis were mainly located in the anterior segment and posterior segment, and the anterior segment had a higher proportion, followed by the posterior segment. Secondary epidermoid cyst of the penis occur mainly in adults. Postoperative wound infection accelerates the appearance of secondary epidermoid cyst of the penis. Patients with phimosis have an increased probability of developing secondary epidermoid cysts of the penis after surgery. The incidence of secondary epidermoid cysts and postoperative infection after manual circumcision by the attending physician was higher than that of the chief physician. Circumcision, injection of anesthesia at the base of the penis, ligation of the penis, and postoperative wound infection may be the etiologies and triggers of secondary epidermoid cysts of the penis. Adults and phimosis patients may be high-risk groups. Lower-level surgeons may increase the odds of postoperative secondary epidermoid cysts of the penis, and it is recommended that surgery be performed by a clinically-experienced, higher-level surgeon. The indications for circumcision should be strictly evaluated and the operation should be performed as soon as possible, and the less invasive surgical method and anesthesia method should be selected. Reduce irrelevant operations during surgery and avoid wound infection after surgery.
Digit ratio (2D:4D) and maternal testosterone-to-estradiol ratio measured in early pregnancy
Authors: Richards Gareth , Aydin Ezra , Tsompanidis Alex , Padaigaitė Eglė , Austin Topun , Allison Carrie , Holt Rosemary , Baron-Cohen Simon
Published: August 2022

The ratio of index to ring finger (2D:4D) has been hypothesised to indicate prenatal androgen exposure, yet evidence for its validity is lacking. We report the first pre-registered study to investigate mothers’ early pregnancy sex hormone concentrations in relation to their children’s digit ratios measured at 18–22-month follow-up. Although the testosterone (T) to estradiol (E) ratio correlated negatively with right hand digit ratio (R2D:4D) and directional asymmetry (right-minus-left) in digit ratio (D[R−L]), neither effect remained statistically significant once demographic and obstetric covariates were controlled for. Nevertheless, the multivariate level of analysis did reveal that T correlated positively with left hand digit ratio (L2D:4D) and negatively with D[R−L]. However, the first of these effects is in the opposite direction to that predicted by theory. Taken together, the results of our study suggest research with larger samples is required to determine whether digit ratios are valid proxies for maternal sex hormone exposure.
Unveiling four decades of intensifying precipitation from tropical cyclones using satellite measurements
Authors: Shearer Eric J. , Afzali Gorooh Vesta , Nguyen Phu , Hsu Kuo-Lin , Sorooshian Soroosh
Published: August 2022

Increases in precipitation rates and volumes from tropical cyclones (TCs) caused by anthropogenic warming are predicted by climate modeling studies and have been identified in several high intensity storms occurring over the last half decade. However, it has been difficult to detect historical trends in TC precipitation at time scales long enough to overcome natural climate variability because of limitations in existing precipitation observations. We introduce an experimental global high-resolution climate data record of precipitation produced using infrared satellite imagery and corrected at the monthly scale by a gauge-derived product that shows generally good performance during two hurricane case studies but estimates higher mean precipitation rates in the tropics than the evaluation datasets. General increases in mean and extreme rainfall rates during the study period of 1980–2019 are identified, culminating in a 12–18%/40-year increase in global rainfall rates. Overall, all basins have experienced intensification in precipitation rates. Increases in rainfall rates have boosted the mean precipitation volume of global TCs by 7–15% over 40 years, with the starkest rises seen in the North Atlantic, South Indian, and South Pacific basins (maximum 59–64% over 40 years). In terms of inland rainfall totals, year-by-year trends are generally positive due to increasing TC frequency, slower decay over land, and more intense rainfall, with an alarming increase of 81–85% seen from the strongest global TCs. As the global trend in precipitation rates follows expectations from warming sea surface temperatures (11.1%/°C), we hypothesize that the observed trends could be a result of anthropogenic warming creating greater concentrations of water vapor in the atmosphere, though retrospective studies of TC dynamics over the period are needed to confirm.
Matrix stiffness and architecture drive fibro-adipogenic progenitors’ activation into myofibroblasts
Authors: Loomis Taryn , Hu Lin-Ya , Wohlgemuth Ross P. , Chellakudam Rosemary R. , Muralidharan Pooja D. , Smith Lucas R.
Published: August 2022

Fibro-adipogenic progenitors (FAPs) are essential in supporting regeneration in skeletal muscle, but in muscle pathologies FAPs the are main source of excess extracellular matrix (ECM) resulting in fibrosis. Fibrotic ECM has altered mechanical and architectural properties, but the feedback onto FAPs of stiffness or ECM properties is largely unknown. In this study, FAPs’ sensitivity to their ECM substrate was assessed using collagen coated polyacrylamide to control substrate stiffness and collagen hydrogels to engineer concentration, crosslinking, fibril size, and alignment. FAPs on substrates of fibrotic stiffnesses had increased myofibroblast activation, depicted by αSMA expression, compared to substrates mimicking healthy muscle, which correlated strongly YAP nuclear localization. Surprisingly, fibrosis associated collagen crosslinking and larger fibril size inhibited myofibroblast activation, which was independent of YAP localization. Additionally, collagen crosslinking and larger fibril diameters were associated with decreased remodeling of the collagenous substrate as measured by second harmonic generation imaging. Inhibition of YAP activity through verteporfin reduced myofibroblast activation on stiff substrates but not substrates with altered architecture. This study is the first to demonstrate that fibrotic muscle stiffness can elicit FAP activation to myofibroblasts through YAP signaling. However, fibrotic collagen architecture actually inhibits myofibroblast activation through a YAP independent mechanism. These data expand knowledge of FAPs sensitivity to ECM and illuminate targets to block FAP’s from driving progression of muscle fibrosis.
The quality-adjusted life-years in the oncological patients’ health-related quality of life
Authors: Kucnerowicz Karolina , Pietrzak Agata , Cholewiński Witold , Martenka Piotr , Marszałek Andrzej , Burchardt Ewa , Strzesak Erwin
Published: August 2022

The oncological treatment can significantly affect patients’ health-related quality of life (HRQoL), which should be monitored to ensure our patients’ well-being. The often-used HRQoL measurer is the quality-adjusted life-year (QALY) indicator of the disease burden, describing both quality and quantity of life lived. The main aim of the study was to discuss the methodology and usefulness of evaluating QALYs using the HRQoL questionnaires: EuroQoL (EQ)-5 dimensions-3 levels (EQ-5D-3L) and EQ visual analogue scale (EQ-VAS) in 32 cervical cancer patients. We obtained the questionnaire and calculated QALYs based on the Gross Domestic Product (GDP) method. In our study, the total scoring of the EQ-Index, EQ-VAS evaluation was 2620 and 2409 points, respectively, which corresponds with the QALYs value of 26.2 and 24.9, respectively. We expressed the QALYs outcome into the economic equivalent of nearly 900,000 US dollars (USD) as the total health profit for both the patients and the healthcare system. Obtaining the QALY factor can help establish the medical management’s influence on the patients’ HRQoL and improve the healthcare services to ensure the best health outcomes.
Quality assessment and chemical diversity of Australian propolis from Apis mellifera bees
Authors: Tran Chau T. N. , Brooks Peter R. , Bryen Tahmikha J. , Williams Simon , Berry Jessica , Tavian Fiona , McKee Ben , Tran Trong D.
Published: August 2022

The propolis industry is well established in European, South American and East Asian countries. Within Australia, this industry is beginning to emerge with a few small-scale producers. To contribute to the development of the Australian propolis industry, the present study aimed to examine the quality and chemical diversity of propolis collected from various regions across Australia. The results of testing 158 samples indicated that Australian propolis had pure resin yielding from 2 to 81% by weight, total phenolic content and total flavonoid content in one gram of dry extract ranging from a few up to 181 mg of gallic acid equivalent and 145 mg of quercetin equivalent, respectively. Some Australian propolis showed more potent antioxidant activity than the well-known Brazilian green, Brazilian red, and Uruguayan and New Zealand poplar-type propolis in an in vitro DPPH assay. In addition, an HPLC–UV analysis resulted in the identification of 16 Australian propolis types which can be considered as high-grade propolis owing to their high total phenolic content. Chemometric analysis of their 1H NMR spectra revealed that propolis originating from the eastern and western coasts of Australia could be significantly discriminated based on their chemical composition.
C-terminal truncation modulates α-Synuclein’s cytotoxicity and aggregation by promoting the interactions with membrane and chaperone
Authors: Zhang Cai , Pei Yunshan , Zhang Zeting , Xu Lingling , Liu Xiaoli , Jiang Ling , Pielak Gary J. , Zhou Xin , Liu Maili , Li Conggang
Published: August 2022

α-Synuclein (α-syn) is the main protein component of Lewy bodies, the major pathological hallmarks of Parkinson’s disease (PD). C-terminally truncated α-syn is found in the brain of PD patients, reduces cell viability and tends to form fibrils. Nevertheless, little is known about the mechanisms underlying the role of C-terminal truncation on the cytotoxicity and aggregation of α-syn. Here, we use nuclear magnetic resonance spectroscopy to show that the truncation alters α-syn conformation, resulting in an attractive interaction of the N-terminus with membranes and molecular chaperone, protein disulfide isomerase (PDI). The truncated protein is more toxic to mitochondria than full-length protein and diminishes the effect of PDI on α-syn fibrillation. Our findings reveal a modulatory role for the C-terminus in the cytotoxicity and aggregation of α-syn by interfering with the N-terminus binding to membranes and chaperone, and provide a molecular basis for the pathological role of C-terminal truncation in PD pathogenesis.
Quantum critical fluctuations in an Fe-based superconductor
Authors: Jost Daniel , Peis Leander , He Ge , Baum Andreas , Geprägs Stephan , Palmstrom Johanna C. , Ikeda Matthias S. , Fisher Ian R. , Wolf Thomas , Lederer Samuel , Kivelson Steven A. , Hackl Rudi
Published: August 2022

Quantum critical fluctuations may prove to play an instrumental role in the formation of unconventional superconductivity. Here, we show that the characteristic scaling of a marginal Fermi liquid is present in inelastic light scattering data of an Fe-based superconductor tuned through a quantum critical point (QCP) by chemical substitution or doping. From the doping dependence of the imaginary time dynamics we are able to distinguish regions dominated by quantum critical behavior from those having classical critical responses. This dichotomy reveals a connection between the marginal Fermi liquid behavior and quantum criticality. In particular, the overlap between regions of high superconducting transition temperatures and quantum critical scaling suggests a contribution from quantum fluctuations to the formation of superconductivity.
Unexpected high carbon losses in a continental glacier foreland on the Tibetan Plateau
Authors: Zhang Jiejie , Ma Anzhou , Zhou Hanchang , Chen Xianke , Zhou Xiaorong , Liu Guohua , Zhuang Xuliang , Qin Xiang , Priemé Anders , Zhuang Guoqiang
Published: August 2022

Closely related with microbial activities, soil developments along the glacier forelands are generally considered a carbon sink; however, those of continental glacier forelands remain unclear. Continental glaciers are characterized by dry conditions and low temperature that limit microbial growth. We investigated the carbon characteristics along a chronosequence of the Laohugou Glacier No. 12 foreland, a typical continental glacier on the Tibetan Plateau, by analyzing soil bacterial community structure and microbial carbon-related functional potentials. We found an unexpected carbon loss in which soil organic carbon decreased from 22.21 g kg−1 to 10.77 g kg−1 after receding 50 years. Structural equation modeling verified the important positive impacts from bacterial community. Lower carbon fixation efficiency along the chronosequence was supported by less autotrophic bacteria and carbon fixation genes relating to the reductive tricarboxylic acid cycle. Lower carbon availability and higher carbon requirements were identified by an increasing bacterial copy number and a shift of the dominant bacterial community from Proteobacteria and Bacteroidetes (r-strategists) to Actinobacteria and Acidobacteria (K-strategists). Our findings show that the carbon loss of continental glacier foreland was significantly affected by the changes of bacterial community, and can help to avoid overestimating the carbon sink characteristics of glacier forelands in climate models.
High-efficiency and high-fidelity ssDNA circularisation via the pairing of five 3′-terminal bases to assist LR-LAMP for the genotyping of single-nucleotide polymorphisms
Authors: Li Taiwen , Zou Huayan , Zhang Jing , Ding Haixia , Li Cheng , Chen Xiangru , Li Yunzhou , Feng Wenzhuo , Kageyama Koji
Published: August 2022

A high-fidelity ssDNA circularisation via the pairing of five 3′-terminal bases was developed to assist LR-LAMP for genotyping of SNPs.
Development and validation of a label-free colorimetric aptasensor based on the HCR and hemin/G-quadruplex DNAzyme for the determination of patulin in fruits and fruit-based products from Xinjiang (China)
Authors: Lu Chunxia , Chen Xia , Ji Yong , Liu Chengjiang , Liu Changbin
Published: August 2022

In this study, a simple and practical label-free colorimetric aptasensor was successfully prepared for the ultrasensitive detection of patulin, based on the hybridization chain reaction and hemin/G-quadruplex DNAzyme-signal amplification strategy.
Construction of a matchstick-shaped Au@ZnO@SiO2–ICG Janus nanomotor for light-triggered synergistic antibacterial therapy
Authors: Liu Xuan , Liu Huaxiao , Zhang Jinzha , Hao Yijie , Yang Hongna , Zhao Wenbo , Mao Chun
Published: August 2022

Nanomotors can provide support for increasing the action radius of ROS and improving the membrane insertion ability of a photosensitizer.