bluuebunny/crossref_metadata_2025 huggingface dataset:
The Synergistic Cytotoxic Effects of GW5074 and Sorafenib by Impacting Mitochondrial Functions in Human Colorectal Cancer Cell Lines
Authors: Hu Je-Ming , Chang Yung-Lung , Hsieh Cheng-Chih , Huang Shih-Ming
Published: June 2022

Colorectal cancer (CRC) ranks third in the United States for incidence or mortality. Surgical resection is the primary treatment for patients at an early stage, while patients with advanced and metastatic CRC receive combined treatment with chemotherapy, radiotherapy, or targeted therapy. C-RAF plays a key role in maintaining clonogenic and tumorigenic capacity in CRC cells and it might be a potential therapeutic target for CRC. Sorafenib is a popular oral multi-kinase inhibitor, including a B-RAF inhibitor that targets the RAF-MEK-ERK pathway. Sorafenib, as a single agent, has tumor-suppressing efficacy, but its clinical application is limited due to many complex drug resistance mechanisms and side effects. GW5074 is one of the C-RAF inhibitors and has the potential to enhance the efficacy of existing cancer chemotherapies. In this study, we investigated whether the combination of sorafenib with GW5074 could reduce the dosage of sorafenib and enhance its tumor-suppressive effect in two CRC cell lines, HCT116 and LoVo cells. Our findings demonstrate that GW5074 can potentiate the cytotoxicity of sorafenib and dramatically reduce the half-maximal inhibitory concentration (IC50) dose of sorafenib from 17 and 31 µM to 0.14 and 0.01 µM in HCT116 and LoVo cells, respectively. GW5074, similar to sorafenib, suppressed the cellular proliferation and induced cellular apoptosis and cytosolic ROS, but had no further enhancement on the above-mentioned effects when combined with sorafenib. The synergistic effects of GW5074 and sorafenib were mainly found in mitochondrial functions, including ROS generation, membrane potential disruption, and fission–fusion dynamics, which were examined by using the flow cytometry analysis. In summary, the C-RAF inhibitor GW5074 might potentiate the cytotoxicity of the B-RAF inhibitor sorafenib mediated through mitochondrial dysfunctions, suggesting that GW5074 potentially serves as a sensitizer for sorafenib application to reduce the risk of drug resistance of CRC treatment. Our findings also provide novel insights on using C-RAF inhibitors combined with sorafenib, the current CRC therapeutic drug choice, in CRC treatment.
Effect of Wearing Running Shoes on Lower Limb Kinematics by Using OpenSim Simulation Software
Authors: Renganathan Gunarajulu , Barnamehei Hamidreza , Das Swagata , Kurita Yuichi
Published: June 2022

(1) Flatfoot is a common malformation in both children and adults, in which a proper arch fails to develop. This study aimed to see how over-the-counter running shoes improved the gait patterns of flatfoot patients. (2) Methods: Three healthy flatfoot subjects were included in the study. Flatfoot was diagnosed by a lateral talometatarsal angle of more than 4 degrees and a talocalcaneal angle of more than 30 degrees. All the patient data were captured using Vicon motion caption cameras. The subjects were allowed to walk at self-selected speeds with and without running shoes. (3) Results: Significant differences in lower limb kinematics were observed between barefoot and running shoe gait. In addition, by wearing the running shoes, the center of mass and lower limb kinematics changed. (4) Conclusion: The improvement in balance and control was clearly indicated, and the change in gait on the entire lower limb influenced normalizing the stresses of the foot with running shoes. These valuable results can be used for rehabilitation programs.
Potential Probiotic Acceptability of a Novel Strain of Paenibacillus konkukensis SK 3146 and Its Dietary Effects on Growth Performance, Intestinal Microbiota, and Meat Quality in Broilers
Authors: Moon Seung-Gyu , Kothari Damini , Lee Woo-Do , Kim Jong-Il , Kim Kyung-Il , Kim Yong-Gi , Ga Gun-Whi , Kim Eun-Jip , Kim Soo-Ki
Published: June 2022

This study evaluates the in vitro probiotic characteristics of P. konkukensis sp. nov. SK-3146, which was isolated from animal feed, and its dietary effects on growth performance, intestinal characteristics, intestinal microbiota, and meat quality in broilers. In vitro experiments revealed that P. konkukensis was non-hemolytic with variable antibiotic susceptibility, and acid as well as bile tolerance. To assess the effect of P. konkukensis on broilers, a total of four hundred eighty 1-day-old Ross 308 broiler chicks were allocated to 3 treatment groups with 4 replicates of 40 birds each; the negative control group was fed a basal diet without any feed additives (NC), the positive control group was fed a basal diet containing 0.01% enramycin (PC), and the experimental group was fed a basal diet containing P. konkukensis bacterial culture (PK) at 104 CFU/g of the diet based on bacterial count. The experiment lasted for 35 days. Results indicated that there were no significant differences in any growth performance parameters among the dietary treatments (p > 0.05). In addition, the inclusion of P. konkukensis in the broilers’ diet did not affect meat cooking loss, color, and pH but increased the relative weight of breast meat (p < 0.05). The PK group showed heavier intestinal weight and shorter intestinal length than the NC group (p < 0.05). The ratio of the intestinal weight to length of jejunum was the highest in the PK group (p < 0.05). The PK group showed increased counts of Streptococcus thermophilus (p < 0.05) with no adverse effects of P. konkukensis on other intestinal microbiota in the jejunum. This study implies that P. konkukensis might have the potential to be applied as a probiotic feed additive in poultry.
Spectroscopic and Microscopic Characterization of Flashed Glasses from Stained Glass Windows
Authors: Palomar Teresa , Martínez-Weinbaum Marina , Aparicio Mario , Maestro-Guijarro Laura , Castillejo Marta , Oujja Mohamed
Published: June 2022

Flashed glasses are composed of a base glass and a thin colored layer and have been used since medieval times in stained glass windows. Their study can be challenging because of their complex composition and multilayer structure. In the present work, a set of optical and spectroscopic techniques have been used for the characterization of a representative set of flashed glasses commonly used in the manufacture of stained glass windows. The structural and chemical composition of the pieces were investigated by optical microscopy, field emission scanning electron microscopy-energy dispersive X-ray spectrometry (FESEM-EDS), UV-Vis-IR spectroscopy, laser-induced breakdown spectroscopy (LIBS), and laser-induced fluorescence (LIF). Optical microscopy and FESEM-EDS allowed the determination of the thicknesses of the colored layers, while LIBS, EDS, UV-Vis-IR, and LIF spectroscopies served for elemental, molecular, and chromophores characterization of the base glasses and colored layers. Results obtained using the micro-invasive LIBS technique were compared with those retrieved by the cross-sectional technique FESEM-EDS, which requires sample taking, and showed significant consistency and agreement. In addition, LIBS results revealed the presence of additional elements in the composition of flashed glasses that could not be detected by FESEM-EDS. The combination of UV-Vis-IR and LIF results allowed precise chemical identification of chromophores responsible for the flashed glass coloration.
Isolation of Listeria ivanovii from Bulk-Tank Milk of Sheep and Goat Farms—From Clinical Work to Bioinformatics Studies: Prevalence, Association with Milk Quality, Antibiotic Susceptibility, Predictors, Whole Genome Sequence and Phylogenetic Relationships
Authors: Lianou Daphne T. , Skoulakis Anargyros , Michael Charalambia K. , Katsarou Eleni I. , Chatzopoulos Dimitris C. , Solomakos Nikolaos , Tsilipounidaki Katerina , Florou Zoe , Cripps Peter J. , Katsafadou Angeliki I. , Vasileiou Natalia G. C. , Dimoveli Konstantina S. , Bourganou Maria V. , Liagka Dimitra V. , Papatsiros Vasileios G. , Kontou Panagiota I. , Mavrogianni Vasia S. , Caroprese Mariangela , Petinaki Efthymia , Fthenakis George C.
Published: June 2022

A cross-sectional study was performed in 325 sheep and 119 goat dairy farms in Greece. Samples of bulk-tank milk were examined by standard microbiological techniques for Listeria spp. Listeria monocytogenes was isolated from one (0.3%) and Listeria ivanovii from three (0.9%) sheep farms. No associations between the isolation of L. monocytogenes or L. ivanovii and milk quality were found. No resistance to antibiotics was identified. Three variables emerged as significant predictors of isolation of the organism: the presence of pigs, low average relative humidity and a high number of ewes on the farm. The three L. ivanovii isolates were assessed in silico for identification of plasmids, prophages, antibiotic resistance genes, virulence factors, CRISPRs and CAS genes. Phylogenetic analysis using the core genome revealed that the three strains belonged to the L. ivanovii subsp. ivanovii branch and were especially close to the PAM 55 strain. All strains of the branch appeared to be very similar, with the distance between them being small.
Reproductive Biology of Dry Grassland Specialist Ranunculus illyricus L. and Its Implications for Conservation
Authors: Kocot Dawid , Sitek Ewa , Nowak Barbara , Kołton Anna , Towpasz Krystyna
Published: June 2022

Ranunculus illyricus, a component of xerothermic grasslands, is a declining species and deserves active conservation treatments in many countries preceded by studies on the biology of its reproduction. So far, our knowledge of R. illyricus, a species with two modes of reproduction, has been fragmentary. The purpose of the studies presented here was to describe the annual development cycle of R. illyricus with particular emphasis on the production of underground tuber clusters that serve as vegetative propagation. Based on three-year-long observations in an ex situ collection, the efficiency of vegetative propagation was estimated and compared with the efficiency of generative propagation. It was found that in 3 years the best clones could produce up to 57 progeny clusters followed by flowering specimens in the first season. Meanwhile, the high potential for generative reproduction was suppressed by many limitations including fruit setting, the germination capacity of seeds, seedling survival rate, and additionally, the first flowering plant was observed only in the third year. It seems that the efficiency of vegetative propagation of this species can be higher than the efficiency of generative propagation. Moreover, vegets bloomed in the first year after emergence, whereas the first plant of generative origin was observed to bloom only after 3 years. A large proportion of individuals of vegetative origin can negatively affect the genetic diversity of the population but their survival rate against competing plants is higher. To enhance the existing populations or to create new ones, it would be best to use plants derived from clonal propagation of genets carried out in ex situ conditions.
Utility of iPSC-Derived Cells for Disease Modeling, Drug Development, and Cell Therapy
Authors: Nicholson Martin W. , Ting Chien-Yu , Chan Darien Z. H. , Cheng Yu-Che , Lee Yi-Chan , Hsu Ching-Chuan , Huang Ching-Ying , Hsieh Patrick C. H.
Published: June 2022

The advent of induced pluripotent stem cells (iPSCs) has advanced our understanding of the molecular mechanisms of human disease, drug discovery, and regenerative medicine. As such, the use of iPSCs in drug development and validation has shown a sharp increase in the past 15 years. Furthermore, many labs have been successful in reproducing many disease phenotypes, often difficult or impossible to capture, in commonly used cell lines or animal models. However, there still remain limitations such as the variability between iPSC lines as well as their maturity. Here, we aim to discuss the strategies in generating iPSC-derived cardiomyocytes and neurons for use in disease modeling, drug development and their use in cell therapy.
Efficacy of Topical Intervention for Recurrent Aphthous Stomatitis: A Network Meta-Analysis
Authors: Liu Hao , Tan Lei , Fu Gege , Chen Ling , Tan Hua
Published: June 2022

Background and objectives: To compare the efficacy and safety of topical interventions used for recurrent aphthous stomatitis. Materials and Methods: This network meta-analysis was conducted in accordance with the PRISMA statement. We searched four electronic databases, PubMed, Web of Science (WOS), Cochrane Central Register of Controlled Trials and Embase, for randomized controlled trials reporting efficacy and safety data on topical interventions for recurrent aphthous stomatitis. We performed a quality evaluation using a methodology based on the Cochrane Handbook. Two authors independently extracted data on healing effect, size reduction effect, symptom reduction effect, recurrence and safety assessment. Network meta-analysis was then performed using ADDIS and RevMan. Results: A total of 72 trials (5272 subjects) involving 29 topical interventions were included. Honey, lnsulin liposome gel, laser, amlexanox, glycyrrhiza and triamcinolone had better efficacy performance. Probiotics and chlorhexidine helped to prolong ulcer intervals and reduce recurrence. Doxycycline and penicillin had a high risk of adverse events. Hematologic evaluation showed no preference. The rank possibility of size-reducing effect and symptom-reducing effect supported the short-term effect of laser and the long-term effect of probiotics. Conclusions: We recommend the use of laser as a short-term intervention during the exacerbation phase of RAS and probiotics as a long-term intervention during the exacerbation and remission phases of RAS.
A Study on Friction Stir Processing Parameters of Recycled AA 6063/TiO2 Surface Composites for Better Tribological Performance
Authors: Teo Guo Sheng , Liew Kia Wai , Kok Chee Kuang
Published: June 2022

This work aims to determine and select the suitable friction stir processing parameters for recycled aluminum alloy 6063 surface composites reinforced with titanium dioxide for better tribological performance. A medium range of processing parameters (1200–2000 rpm, 25–45 mm/min) were used to compare with a unique relatively high rotational speed of 2442 rpm and feed rate of 50 mm/min for the sample fabrication. The surface composites’ microhardness was measured and the friction and wear performance were tested using the pin-on-disc tribo-tester under starved lubrication conditions. The results show that surface composites produced at a high rotational speed of 2442 rpm and feed rate of 50 mm/min improved 45% in surface microhardness and reduced the friction coefficient and wear rate by 39% and 73%, respectively, compared to the substrate material.
Contactless Micro-Droplet Manipulation of Liquid Released from a Parallel Plate to an Open Region in Electrowetting-on-Dielectric Platform
Authors: Chang Yii-Nuoh , Yao Da-Jeng
Published: June 2022

In electrowetting-on-dielectric (EWOD) platform, the transfer of droplets from the EWOD boundary region (top plate and bottom plate) to the open region is challenging. The challenge is due to the resistance-like surface tension, friction from the top-plate edge, and the so-called boundary. For this purpose, we designed the top plate to minimize the friction resistance at the boundary. The experiment focused on Gibb’s formula and successfully transferred the liquid droplet between the top plate and bottom plate boundary region under a high voltage environment. The threshold voltage for the successful transportation of the droplet between the boundary is 250 V which provides strong pressure to drive the droplet.
Supramolecular Aggregation of Nanoparticles on Aluminum and Gold Surfaces Occurring in Bovine Serum Albumin Solutions
Authors: Salanov Aleksei , Serkova Alexandra , Zhirnova Anastasia , Perminova Larisa , Kovalenko Galina
Published: June 2022

The supramolecular aggregation processes occurring on metallic (aluminum and gold) surfaces in aqueous solutions of bovine serum albumin (BSA) during drying were studied using advanced scanning electron microscopy (SEM). The possible mechanism for the formation of amazing intricate fractal structures on metallic surfaces was proposed based on the analysis of SEM images, size distribution diagrams and EDX-scanning element distribution maps.
Efficient Separation of Ultrafine Coal Assisted by Selective Adsorption of Polyvinylpyrrolidone
Authors: Mei Yujie , Lin Qiuyu , Wu Changning , Huang Wei , Cao Daofan , Liu Ke
Published: June 2022

The efficient separation of ultrafine coal is a challenging process due to the ultrafine particle size and gangue entrainment. In this study, a polymer, polyvinylpyrrolidone (PVP), was introduced as a regulator for ultrafine coal beneficiation. The addition of PVP improved the combustible recovery of clean coal and decreased the ash content. This effect was also presented by the selectivity index. The regulation mechanism of PVP was investigated using diverse methods. The adsorption tests performed demonstrated the adsorption amount of PVP on coal, kaolinite, and quartz, which were related to the increase in the separation efficiency. A zeta potential analyzer was employed to elucidate the effect of PVP on the electrical properties of ultrafine particles. The results revealed that the electrokinetic potential of mineral was sensitive to the varying PVP concentration. The particle size distribution was observed to value the influence of PVP on the particle behavior, which was tested by a laser particle size analyzer. X-ray photoelectron spectroscopy was used to investigate the surface elemental compositions of coal, kaolinite, and quartz, which were regulated by the adsorption of PVP. This research is beneficial to understanding the role of PVP as regulators and provides a basis for the efficient separation of ultrafine coal.
Thermal Transformation of Natural Schwertmannite in the Presence of Chromium
Authors: Lázaro Carlos , Antelo Juan , Carabante Ivan , Otero-Fariña Alba , Verdes Pedro V. , Dacunha-Marinho Bruno , Fiol Sarah
Published: June 2022

Schwertmannite is a metastable mineral playing a crucial role in the immobilization of metal(oid)s in acid mine drainage (AMD) systems. High temperatures associated with wildfires could lead to a sudden schwertmannite transformation, changing the mobility of metal(oid)s. The objective of the present study was to examine the thermal transformation from schwertmannite to hematite, and the subsequent effect on the chromium partitioning. The immobilization of arsenate after thermal transformation and its implications on chromium mobility was also evaluated. Natural schwertmannite, with increasing contents of chromium, was thermally treated between 200 to 800 °C. Transformation products were characterized by solid-phase techniques and selective chemical extractions. Results indicated a transformation to hematite at temperatures above 400 °C. The presence of chromium barely affected the temperature at which the transformation occurred, although partitioning of chromium in the mineral changed with temperature. As the temperature increased from 25 °C to 400 °C, chromium was less mobile and less outcompeted by arsenic adsorption, suggesting a larger contribution of inner-sphere complexes with increasing temperature. At temperatures above 600 °C, non-mobile forms strongly associated with neo-formed hematite were found. Finally, neo-formation of hematite led to a decrease in arsenic adsorption, implying a potentially enhanced arsenic mobility in AMD systems upon wildfires.
Pre-Processing to Increase the Capacity of SAG Mill Circuits—Case Study
Authors: Delboni Homero , Costa e Silva Evandro , Alves Vladmir Kronemberger , Chieregati Ana Carolina
Published: June 2022

This paper describes the adopted approach for increasing the capacity of an existing industrial grinding circuit by adapting the respective configuration to process the ore from a new mine. Accordingly, due to Sossego mine exhaustion, Vale S. A. decided to use the existing industrial facilities and infrastructure for processing the Cristalino ore deposit located in Para state, within the Brazilian Amazon. Considering the higher hardness of Cristalino ore compared to Sossego ore, a reduction in capacity in the existing SAG grinding circuit was anticipated. A comprehensive grinding pilot plant campaign was conducted with a characterization program including 98 Cristalino ore samples, as described throughout this paper. Sossego grinding circuit was also surveyed for mathematical modeling and simulations to assess such an estimative further. The mathematical model calibration for setting different circuit configurations and operating conditions to enhance the circuit’s capacity was based on the combination of pilot plant results and ore characterization. Simulations indicated that a capacity increase of 12% would be achieved in the existing grinding circuit by further crushing 35% of SAG mill fresh feed. Such figures would represent yearly additions of 8.3 kt in copper and 250 kg in gold productions.
Influence of Open Chain and Cyclic Structure of Peptidomimetics on Antibacterial Activity in E. coli Strains
Authors: Sahrawat Parul , Kowalczyk Paweł , Koszelewski Dominik , Szymczak Mateusz , Kramkowski Karol , Wypych Aleksandra , Ostaszewski Ryszard
Published: June 2022

An efficient method for the synthesis of functionalized peptidomimetics via multicomponent Ugi reaction has been developed. The application of trifluoroethanol (TFE) as a reaction medium provided desired products with good yields. Further, using the developed cyclisation reaction, the obtained peptidomimetics were transformed into the cyclic analogues (diketopiperazines, DKPs). The goal of the performed studies was to revised and compare whether the structure of the obtained structurally flexible acyclic peptidomimetics and their rigid cycling analogue DKPs affect antimicrobial activity. We studied the potential of synthesized peptidomimetics, both cyclic and acyclic, as antimicrobial drugs on model E. coli bacteria strains (k12, R2–R4). The biological assays reveal that DKPs hold more potential as antimicrobial drugs compared to open chain Ugi peptidomimetics. We believe that it can be due to the rigid cyclic structure of DKPs which promotes the membrane penetration in the cell of studied pathogens. The obtained data clearly indicate the high antibiotic potential of synthesized diketopiperazine derivatives over tested antibiotics.
3D Graphene Foam by Chemical Vapor Deposition: Synthesis, Properties, and Energy-Related Applications
Authors: Banciu Cristina Antonela , Nastase Florin , Istrate Anca-Ionela , Veca Lucia Monica
Published: June 2022

In this review, we highlight recent advancements in 3D graphene foam synthesis by template-assisted chemical vapor deposition, as well as their potential energy storage and conversion applications. This method offers good control of the number of graphene layers and porosity, as well as continuous connection of the graphene sheets. The review covers all the substrate types, catalysts, and precursors used to synthesize 3D graphene by the CVD method, as well as their most viable energy-related applications.
Correction: Jiao et al. Effects of Fusarium solani and F. oxysporum Infection on the Metabolism of Ginsenosides in American Ginseng Roots. Molecules 2015, 20, 10535–10552
Authors: Jiao Xiaolin , Lu Xiaohong , Luo Yi , Hao Jianjun J. , Gao Weiwei
Published: June 2022

A reader recently brought to the attention of the Editor-in-Chief and the Editorial Office of Molecules several errors in our paper [...]
Design, Development, and a Pilot Study of a Low-Cost Robot for Child–Robot Interaction in Autism Interventions
Authors: Katsanis Ilias A. , Moulianitis Vassilis C. , Panagiotarakos Diamantis T.
Published: June 2022

Socially assistive robots are widely deployed in interventions with children on the autism spectrum, exploiting the benefits of this technology in social behavior intervention plans, while reducing their autistic behavior. Furthermore, innovations in modern technologies such as machine learning enhance these robots with great capabilities. Since the results of this implementation are promising, their total cost makes them unaffordable for some organizations while the needs are growing progressively. In this paper, a low-cost robot for autism interventions is proposed, benefiting from the advantages of machine learning and low-cost hardware. The mechanical design of the robot and the development of machine learning models are presented. The robot was evaluated by a small group of educators for children with ASD. The results of various model implementations, together with the design evaluation of the robot, are encouraging and indicate that this technology would be advantageous for deployment in child–robot interaction scenarios.
Silver Nanowires in Stretchable Resistive Strain Sensors
Authors: Raman Srinivasan , Arunagirinathan Ravi Sankar
Published: June 2022

Silver nanowires (AgNWs), having excellent electrical conductivity, transparency, and flexibility in polymer composites, are reliable options for developing various sensors. As transparent conductive electrodes (TCEs), AgNWs are applied in optoelectronics, organic electronics, energy devices, and flexible electronics. In recent times, research groups across the globe have been concentrating on developing flexible and stretchable strain sensors with a specific focus on material combinations, fabrication methods, and performance characteristics. Such sensors are gaining attention in human motion monitoring, wearable electronics, advanced healthcare, human-machine interfaces, soft robotics, etc. AgNWs, as a conducting network, enhance the sensing characteristics of stretchable strain-sensing polymer composites. This review article presents the recent developments in resistive stretchable strain sensors with AgNWs as a single or additional filler material in substrates such as polydimethylsiloxane (PDMS), thermoplastic polyurethane (TPU), polyurethane (PU), and other substrates. The focus is on the material combinations, fabrication methods, working principles, specific applications, and performance metrics such as sensitivity, stretchability, durability, transparency, hysteresis, linearity, and additional features, including self-healing multifunctional capabilities.
Correction: Khan et al. High Mobility Graphene on EVA/PET. Nanomaterials 2022, 12, 331
Authors: Khan Munis , Indykiewicz Kornelia , Tam Pui Lam , Yurgens August
Published: June 2022

The authors wish to make following corrections in this paper [...]
Erbium-Doped GQD-Embedded Coffee-Ground-Derived Porous Biochar for Highly Efficient Asymmetric Supercapacitor
Authors: Bui Thi Ai Ngoc , Huynh Trung Viet , Tran Hai Linh , Doong Ruey-an
Published: June 2022

A nanocomposite with erbium-doped graphene quantum dots embedded in highly porous coffee-ground-derived biochar (Er-GQD/HPB) was synthesized as a promising electrode material for a highly efficient supercapacitor. The HPB showed high porosity, with a large surface area of 1295 m2 g−1 and an average pore size of 2.8 nm. The 2–8-nanometer Er-GQD nanoparticles were uniformly decorated on the HPB, subsequently increasing its specific surface area and thermal stability. Furthermore, the intimate contact between the Er-GQDs and HPB significantly reduced the charge-transfer resistance and diffusion path, leading to the rapid migration of ions/electrons in the mesoporous channels of the HPB. By adding Er-GQDs, the specific capacitance was dramatically increased from 337 F g−1 for the pure HPB to 699 F g−1 for the Er-GQD/HPB at 1 A g−1. The Ragone plot of the Er-GQD/HPB exhibited an ultrahigh energy density of 94.5 Wh kg−1 and a power density of 1.3 kW kg−1 at 1 A g−1. Furthermore, the Er-GQD/HPB electrode displayed excellent cycling stability, and 81% of the initial capacitance remained after 5000 cycles. Our results provide further insights into a promising supercapacitance material that offers the benefits of both fast ion transport from highly porous carbons and electrocatalytic improvement due to the embedment of Er-doped GQDs to enhance energy density relative to conventional materials.
The Association of Vitamin D and Its Pathway Genes’ Polymorphisms with Hypertensive Disorders of Pregnancy: A Prospective Cohort Study
Authors: Si Shuting , Mo Minjia , Cheng Haoyue , Peng Zhicheng , Alifu Xialidan , Zhou Haibo , Chi Peihan , Zhuang Yan , Yu Yunxian
Published: June 2022

Objective: We aimed to explore the effect of single nucleotide polymorphism (SNP) in the genes of the vitamin D (VitD) metabolic pathway and its interaction with VitD level during pregnancy on the development of hypertensive disorders of pregnancy (HDP). Methods: The study was conducted in the Zhoushan Maternal and Child Health Care Hospital, China, from August 2011 to May 2018. The SNPs in VitD metabolic pathway-related genes were genotyped. Plasma 25-hydroxyvitamin vitamin D (25(OH)D) levels was measured at first (T1), second (T2), and third (T3) trimesters. The information of systolic blood pressure (SBP) and diastolic blood pressure (DBP), and the diagnosis of HDP were extracted from the electronic medical record system. Multivariable linear and logistic regression models and crossover analysis were applied. Results: The prospective cohort study included 3699 pregnant women, of which 105 (2.85%) were diagnosed with HDP. After adjusting for potential confounders, VitD deficiency at T2, as well as the change of 25(OH)D level between T1 and T2, were negatively associated with DBP at T2 and T3, but not HDP. Polymorphisms in CYP24A1, GC, and LRP2 genes were associated with blood pressure and HDP. In addition, VitD interacted with CYP24A1, GC, and VDR genes’ polymorphisms on blood pressure. Furthermore, participants with polymorphisms in CYP24A1-rs2248137, LRP2-rs2389557, and LRP2-rs4667591 and who had VitD deficiency at T2 showed an increased risk of HDP. Conclusions: The individual and interactive association between VitD deficiency during pregnancy and SNPs in the genes of the VitD metabolic pathway on blood pressure and HDP were identified.
11β-HSD1 Inhibitor Alleviates Non-Alcoholic Fatty Liver Disease by Activating the AMPK/SIRT1 Signaling Pathway
Authors: Chen Ying , Li Jiali , Zhang Meng , Yang Wei , Qin Wenqi , Zheng Qinzhou , Chu Yanhui , Wu Yan , Wu Dan , Yuan Xiaohuan
Published: June 2022

We investigated the effect of an 11β-HSD1 inhibitor (H8) on hepatic steatosis and its mechanism of action. Although H8, a curcumin derivative, has been shown to alleviate insulin resistance, its effect on non-alcoholic fatty liver disease (NAFLD) remains unknown. Rats were fed a high-fat diet (HFD) for 8 weeks, intraperitoneally injected with streptozotocin (STZ) to induce NAFLD, and, then, treated with H8 (3 or 6 mg/kg/day) or curcumin (6 mg/kg/day) for 4 weeks, to evaluate the effects of H8 on NAFLD. H8 significantly alleviated HFD+STZ-induced lipid accumulation, fibrosis, and inflammation as well as improved liver function. Moreover, 11β-HSD1 overexpression was established by transfecting animals and HepG2 cells with lentivirus, carrying the 11β-HSD1 gene, to confirm that H8 improved NAFLD, by reducing 11β-HSD1. An AMP-activated protein kinase (AMPK) inhibitor (Compound C, 10 μM for 2 h) was used to confirm that H8 increased AMPK, by inhibiting 11β-HSD1, thereby restoring lipid metabolic homeostasis. A silencing-related enzyme 1 (SIRT1) inhibitor (EX572, 10 μM for 4 h) and a SIRT1 activator (SRT1720, 1 μM for 4 h) were used to confirm that H8 exerted anti-inflammatory effects, by elevating SIRT1 expression. Our findings demonstrate that H8 alleviates hepatic steatosis, by inhibiting 11β-HSD1, which activates the AMPK/SIRT1 signaling pathway.
Prognostic Value of Transaminases and Bilirubin Levels at Admission to Hospital on Disease Progression and Mortality in Patients with COVID-19—An Observational Retrospective Study
Authors: Russo Antonio , Pisaturo Mariantonietta , Palladino Roberta , Maggi Paolo , Numis Fabio Giuliano , Gentile Ivan , Sangiovanni Vincenzo , Esposito Vincenzo , Punzi Rodolfo , Calabria Giosuele , Rescigno Carolina , Salomone Megna Angelo , Masullo Alfonso , Manzillo Elio , Russo Grazia , Parrella Roberto , Dell’Aquila Giuseppina , Gambardella Michele , Ponticiello Antonio , Coppola Nicola ,   on behalf of CoviCam Group
Published: June 2022

Introduction: Given the impact of COVID-19 on the world healthcare system, and the efforts of the healthcare community to find prognostic factors for hospitalization, disease progression, and mortality, the aim of the present study was to investigate the prognostic impact of transaminases and bilirubin levels at admission to hospital on disease progression and mortality in COVID-19 patients. Methods: Using the CoviCamp database, we performed a multicenter, observational, retrospective study involving 17 COVID-19 Units in southern Italy. We included all adult patients hospitalized for SARS-CoV-2 infection with at least one determination at hospital admission of aminotransaminases and/or total bilirubin. Results: Of the 2054 patients included in the CoviCamp database, 1641 were included in our study; 789 patients (48%) were considered to have mild COVID-19, 347 (21%) moderate COVID-19, 354 (22%) severe COVID-19, and 151 patients (9%) died during hospitalization. Older age (odds ratio (OR): 1.02; 95% confidence interval (CI) 1.01–1.03), higher Charlson comorbidity index (CCI) (OR 1.088; 95%CI 1.005–1.18), presence of dementia (OR: 2.20; 95% CI: 1.30–3.73), higher serum AST (OR: 1.002; 95% CI: 1.0001–1.004), and total bilirubin (OR: 1.09; 95% CI: 1.002–1.19) values were associated with a more severe clinical outcome. Instead, the 151 patients who died during hospitalization showed a higher serum bilirubin value at admission (OR 1.1165; 95% CI: 1.017–1.335); the same did not apply for AST. Discussion: Patients with COVID-19 with higher levels of AST and bilirubin had an increased risk of disease progression.
Discrepancy between the Actions of Glucagon-like Peptide-1 Receptor Ligands in the Protection of the Heart against Ischemia Reperfusion Injury
Authors: Ismaeil Ali , Babiker Fawzi , Al-Sabah Suleiman
Published: June 2022

Tirzepatide is a dual glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) receptor agonist and a promising therapy for type 2 diabetes mellitus (T2DM). GLP-1 is an incretin hormone with therapeutic potential beyond type 2 diabetes mellitus. However, GLP-1 is rapidly degraded by dipeptdyl peptidase-IV (DPP-IV) to GLP-1 (9-36). Exendin-4 (Ex-4) is a DPP-IV-resistant GLP-1 receptor agonist which, when truncated to Ex-4 (9-39), acts as a GLP-1 receptor antagonist. In the present study, hearts isolated from Wistar rats (n = 8 per group) were perfused with a modified Langendorff preparation. Left ventricular (LV) contractility and cardiovascular hemodynamics were evaluated by a data acquisition program and infarct size was evaluated by 2,3,5-Triphenyl-2H-tetrazolium chloride (TTC) staining and cardiac enzyme levels. Hearts were subjected to 30 min regional ischemia, produced by ligation of the left anterior descending (LAD) coronary artery followed by 30 min reperfusion. Hearts were treated during reperfusion with either the non-lipidated precursor of tirzepatide (NLT), GLP-1, GLP-1 (9-36), or Ex-4 in the presence or absence of Ex-4 (9-39). Infusion of GLP-1 (9-36) or Ex-4 protected the heart against I/R injury (p > 0.01) by normalizing cardiac hemodynamic and enzyme levels. Neither GLP-1, NLT, nor Ex-4 (9-39) showed any protection. Interestingly, Ex-4 (9-39) blocked Ex-4-mediated protection but not that of GLP-1 (9-36). These data suggest that Ex-4-mediated protection is GLP-1-receptor-dependent but GLP-1 (9-36)-mediated protection is not.
Modern Dressings in Prevention and Therapy of Acute and Chronic Radiation Dermatitis—A Literature Review
Authors: Zasadziński Konrad , Spałek Mateusz Jacek , Rutkowski Piotr
Published: June 2022

Radiotherapy is an integral part of modern oncology, applied to more than half of all patients diagnosed with cancer. It can be used alone or in combination with surgery or chemotherapy. However, despite the high precision of radiation delivery, irradiation may affect surrounding healthy tissues leading to the development of toxicity. The most common and clinically significant toxicity of radiotherapy is acute and chronic radiation dermatitis, which could result in desquamation, wounds, nonhealing ulcers, and radionecrosis. Moreover, preoperative radiotherapy impairs wound healing after surgery and may lead to severe wound complications. In this review, we comprehensively discuss available types of dressings used in the management of acute and chronic radiation dermatitis and address their efficacy. The most effective ways of preventing acute radiation dermatitis are film dressings, whereas foam dressings were found effective in its treatment. Data regarding dressings in chronic radiation dermatitis are scarce. This manuscript also contains authors’ consensus.
Therapeutic Potential of Exosomes Derived from Diabetic Adipose Stem Cells in Cutaneous Wound Healing of db/db Mice
Authors: Hsu Hsiang-Hao , Wang Aline Yen Ling , Loh Charles Yuen Yung , Pai Ashwin Alke , Kao Huang-Kai
Published: June 2022

(1) Background: Diabetes impairs angiogenesis and wound healing. Paracrine secretion from adipose stem cells (ASCs) contains membrane-bound nano-vesicles called exosomes (ASC-Exo) but the functional role and therapeutic potential of diabetic ASC-Exo in wound healing are unknown. This study aims to investigate the in vivo mechanistic basis by which diabetic ASC-Exo enhance cutaneous wound healing in a diabetic mouse model. (2) Methods: Topically applied exosomes could efficiently target and preferentially accumulate in wound tissue, and the cellular origin, ASC or dermal fibroblast (DFb), has no influence on the biodistribution pattern of exosomes. In vivo, full-thickness wounds in diabetic mice were treated either with ASC-Exo, DFb-Exo, or phosphate-buffered saline (PBS) topically. ASC-Exo stimulated wound healing by dermal cell proliferation, keratinocyte proliferation, and angiogenesis compared with DFb-Exo and PBS-treated wounds. (3) Results: Diabetic ASC-Exo stimulated resident monocytes/macrophages to secrete more TGF-β1 and activate the TGF-β/Smad3 signaling pathway. Fibroblasts activated by TGF-β1containing exosomes from ASCs initiate the production of TGF-β1 protein in an autocrine fashion, which leads to more proliferation and activation of fibroblasts. TGF-β1 is centrally involved in diabetic ASC-Exo mediated cellular crosstalk as an important early response to initiating wound regeneration. (4) Conclusions: The application of diabetic ASC-Exo informs the potential utility of a cell-free therapy in diabetic wound healing.
A Narrative Review of Studies Comparing Efficacy and Safety of Citalopram with Atypical Antipsychotics for Agitation in Behavioral and Psychological Symptoms of Dementia (BPSD)
Authors: Qasim Haider Saddam , Simpson Maree Donna
Published: June 2022

Background: Psychomotor agitation as part of the behavioral and psychological symptoms of dementia (BPSD) is one of the common issues found in aged care facilities. The current inadequate management strategies lead to poor functional and medical outcomes. Psychotropic interventions are the current preferred treatment method, but should these medications be the prescribers’ first preference? This review aims to compare pharmacological interventions for psychomotor agitation, judging them according to their effectuality and justifiability profiles. This is to be achieved by retrieving information from Randomized Control Trials (RCTs) and systematic reviews. Objectives: This review evaluates evidence from RCTs, systematic reviews, and meta-analyses of BPSD patients who have taken agitation treatments. Assessing the efficacy of citalopram, other selective serotonin reuptake inhibitors (SSRIs) and antipsychotic treatments were compared to each other for the purpose of improving agitation outcomes and lowering patient side effects. Methods: This review includes RCT that compared citalopram with one or more atypical antipsychotics or with a placebo, along with systematic reviews comparing citalopram (SSRI) with antipsychotics such as quetiapine, olanzapine, and risperidone. Studies were extracted by searching and accessing databases, such as PubMed, OVID, and Cochrane with restrictions of date from 2000 to 2021 and published in the English language. Conclusion: There are still a limited number of studies including SSRIs for the treatment of agitation in BPSD. SSRIs such as citalopram were associated with a reduction in the symptoms of agitation, and lower risk of adverse effects when compared to antipsychotics. Future studies are required to assess the long-term safety and efficacy of SSRI treatments for agitation in BPSD.
The Pedagogical and Religious Dimensions of the Rites of the Sacrament of Children’s Baptism
Authors: Słotwińska Helena
Published: June 2022

The topic of the article “The Pedagogical and Religious Dimensions of the Rites of the Sacrament of Baptism for Children” deals with the sacraments in the Catholic Church, particularly baptism as the first of the seven sacraments. As signs, sacraments are also meant to instruct, and indeed they do, for the meaning and grace of baptism are made clear in the rites of its celebration Union with Christ leads to confession of faith in the One God: Father, Son and Holy Spirit. The profession of faith, closely related to baptism, is eminently Trinitarian. The Church baptises: “In the name of the Father and of the Son and of the Holy Spirit” (Matt. 28,19), the Triune God to whom the Christian entrusts his life.The basis for analyzing the rites of baptism will be the Order of Baptism for Children during mass, which contains very important instructions that can be grouped into three points: (1) instructions about God the Father, (2) instructions about the essence of baptism and its importance for the parish and the baptized, (3) instructions about the duties of the baptized and their parents and godparents. In the sacraments God occupies the central place, and the sacrament of baptism, by instructing about God the Father, the first issue, brings closer three fundamental truths about God: (a) God’s initiative in the salvation for man, (b) God’s omnipotence (universal, loving and mysterious), and (c) God’s goodness. The second issue deals with: (a) the essence of baptism based on the terms given in the Rite of Receiving the Children (baptism, faith, the grace of Christ, admission to the Church, and eternal life); (b) the meaning of infant baptism for the parish community; and (c) the meaning of baptism for the child. Likewise, the third issue is also divided into two parts, with an instruction (a) on the duties of the baptized and (b) the duties of baptized children, parents and godparents.
Transforming “Ritual Cultural Features” into “Modern Product Forms”: A Case Study of Ancient Chinese Ritual Vessels
Authors: Sun Yikang , Wu I-Wen , Lin Rungtai
Published: June 2022

Antique relics represent a part of the rich history and cultural heritage of a nation, region, or country, and serve as great inspiration for the creation of unique cultural and artistic products. This article explores the meaning of the transition from “ritual cultural features” to “modern product forms”. By elucidating the rituals (connotation) and forms (denotation) of these cultural characteristics, this article attempts to illustrate how to transform these characteristics into modern products that are tailored to meet the needs of contemporary consumer markets. The specific objects chosen for this study are the “ritual vessels” used in various ceremonial activities in ancient China. Specifically, this study discusses the form of the “ritual vessels”, the meaning of their cultural significance, and their use scenarios. Lastly, this article introduces a cultural product design model based on the relationship between “form” and “ritual”, which seeks to offer a valuable reference for designers of cultural and creative products in the design of modern products that adhere to the continuation of cultural attributes. Moreover, we hope this article will also serve as an inspiration for how designers can use their creative thinking to discover traditional cultures’ advantages.
New Testament Theology: Too Theological, Too Difficult, and Too Repetitive?
Authors: Bird Michael F.
Published: June 2022

This essay argues that New Testament Theology (NTT) is an indispensable mediating discipline between historical exegesis and systematic theology. It defends NTT against claims that: (1) NTT should be replaced by the study of early Christian religion; (2) The NT is too diverse and disparate to sustain any unifying theology; and (3) NTT has been over-done so that there is nothing new to be said. The essay proceeds to the defense of NTT by contending that theology is part of the substance and significance of the NT. In addition, the NT contains several varieties and unities that can simultaneously challenge and aid theological reflection. Finally, the essay proposes a fresh approach to explicate the theological texture and religious impetus of the NT with a view to renewing living faith communities.
The International Responsibility of the Holy See for Human Rights Violations
Authors: Zambrana-Tévar Nicolás
Published: June 2022

In recent years, the Holy See has been accused of violating its human rights obligations because of acts of sex abuse by the Catholic clergy. Such accusations are based, in various ways, on the authority of the Holy See over the clergy. The Holy See is often referred to as a state and its obligations as state obligations. UN treaty bodies understand that the human rights obligations of the Holy See, as a treaty party, are of a legal––not moral––nature and apply extraterritorially, i.e., beyond the territory of the Vatican City State. The notion of state jurisdiction is, therefore, applied to the Holy See in the same way as to any other state. UN treaty bodies implicitly conclude that, for the purpose of human rights responsibility, the crimes of the clergy must be attributed to the Holy See and that anyone under the religious authority of the Holy See is under its state jurisdiction. However, a closer examination of the nature of the Holy See and its authority under international law, church doctrine, and church law paints a more complex and nuanced picture, where it is hard to describe the clergy as state organs or religious authority as state jurisdiction. Still, there are reasons to understand that certain acts and omissions of the Holy See in the context of the sex abuse crisis can be characterized as violations of its positive obligations of conduct.