bluuebunny/crossref_metadata_2025 huggingface dataset:
A Política Nacional de Atenção Integral à Saúde do Homem na Percepção dos Profissionais da Atenção Básica
Authors: Macêdo Maria Ayrlles , Ribeiro Marco Túlio Aguiar Mourão , Oliveira Francisca Bezerra de , Bezerra Marcos Paulo Egídio , Lima Maria Fabiana De Paula , Sousa Paulo Geovane Bezerra de
Published: October 2016

Visando diminuir os índices de mortalidade e as morbidades relacionadas ao homem, o Ministério da Saúde delineia ações e políticas que vislumbram reduzir tais índices e atua diretamente na promoção da saúde, prevenção de doenças, tratamento e cura. Com esse propósito foi criada a Política Nacional de Atenção Integral à Saúde do Homem (PNAISH), sendo instituída pela Portaria nº 1.944/GM, de 27 de agosto de 2009. O presente estudo tem como objetivo compreender como a Política Nacional de Atenção Integral à Saúde do Homem está sendo executada em um munícipio da Região Centro-Sul do Ceará e identificar as ações ofertadas pelo referido município para o público masculino no âmbito da Atenção Básica. Trata-se de uma pesquisa exploratória, com abordagem qualitativa. Para a coleta de dados foram utilizadas entrevistas semiestruturadas com 20 profissionais de nível superior, bem como observação não participante e análise documental. A Análise de dados foi pautada pela Análise de Conteúdo na modalidade de Análise Temática de Bardin. Da análise emergiram dois temas: Dificuldades na execução da Política de Saúde do Homem na Atenção Primária e Saúde do Homem na Atenção Básica: ações a serem implementadas. Os resultados revelam que os profissionais da Atenção Básica têm um pouco conhecimento a respeito da Política de Saúde do Homem, tendo dificuldade de executá-la, ou seja, de colocá-la em prática, devido à falta de capacitação, o que leva à não execução de ações integrais e efetivas à Saúde do Homem   como preconiza o Ministério da Saúde e a pouca oferta de serviços destinados ao público masculino, no município objeto deste estudo.  As ações desenvolvidas em relação à saúde do homem ocorrem de forma pontual, com ênfase no atendimento clínico, prevenção do câncer de próstata e novembro azul.   Os residentes em Saúde da Família e Comunidade da Escola de Saúde Pública do Ceará-ESP têm papel importante nesse processo, devem sensibilizar os profissionais e gestores que ofertam os serviços em saúde, objetivando viabilizar na prática ações concretas no campo da Saúde do Homem, evitando possíveis agravos à saúde nesse grupo.
A Integração e Inclusão do Sujeito com Deficiência no âmbito social e educacional
Authors: Rodrigues Penha Sebastião da Silva , Oliveira Gislene Farias de
Published: October 2016

Inclusão é a nossa capacidade de entender e reconhecer o outro, e assim ter o privilégio de conviver e partilhar com as pessoas diferentes de nós é inclusão. A educação inclusiva deve acolher todas as pessoas sem exceção. Esta pesquisa tem como objetivo descrever sobre a integração e inclusão do sujeito com deficiência no âmbito social e educacional. Para tanto se fez necessário um estudo bibliográfico para embasar todo o desenvolvimento do mesmo, e para enriquecer buscou-se as teorias de Arantes, Ferreira & Ferreira, Fonseca, e entre outros que deram alicerce e sustentação para a construção dos tópicos que estruturam este trabalho, que trata de uma pequena introdução aos conteúdos da pesquisa; um levantamento sobre os conceitos de integração e inclusão, enfatizando a diferença entre um e outro além de destacar a sociedade e o sujeito com deficiência, enfatizando alguns comentários sobres às políticas voltadas para esta população, destacando também a historia da deficiência e o que se entende sobre esse fenômeno.
Desafios e Dificuldades do Professor Alfabetizador
Authors: Morais Maria José Lopes de Sousa , Beserra Theóphilo Michel Álvares Cabral
Published: October 2016

Este trabalho tem como objetivo analisar e discutir questões que permeiam o dia a dia escolar do professor alfabetizador, com ênfase nas dificuldades enfrentadas em sala de aula por esse profissional,já que muitas vezes o mesmo depara-se com obstáculos que limitam o seu trabalho e retardam o processo de ensino aprendizagem dos alunos.Realizou-se aqui uma pesquisa bibliográfica  para considerar suas contribuições teóricas de autores tais como: CAMBI ( 1999), LEMLE (1988), NÉRICI(1972), PIAGET(1997), POERCH(1990)> Podendo-se constatar que alguns dos desafios e dificuldades enfrentados pelo professor são a pouca formação focada na alfabetização, a falta de participação dos pais na vida escolar dos filhos e o alto índice de indisciplina na sala de aula. Diante desses resultados concluiu-se que para superar as dificuldades, o professor deve agir em conjunto com a comunidade escolar e pais para rever Ações e criar outras buscando oferecer uma educação de qualidade ao aluno.
A Terceirização dos Serviços de Administração Pública na Rede Hospitalar e na Educação: Um estudo na visão dos usuários
Authors: Santos Wilian Oliveira , Lopes Júnior Francisco Raimundo , Lima Alexandre Coelho , Lima Camila Coelho , Lima Maria do Socorro Macêdo Coêlho
Published: October 2016

Terceirização é uma expressão muito utilizada nos setores públicos e privados, tendo por significado transferir serviços.  O presente trabalho apresenta questionamentos sobre a terceirização na área da saúde e da educação, com relação à eficiência e satisfação na visão de usuários e funcionários. Em se tratando de saúde, será que a mudança na gestão e a terceirização num hospital público, tem trazido a mesma eficiência ao atendimento dos clientes, na visão dos seus usuários? Ou mesmo, quando se trata da área de educação, o que muda na prestação dos serviços executados por profissionais terceirizados em relação a realização do mesmo serviço pelo profissional efetivo, na visão desse trabalhador? A pesquisa objetiva analisar as vantagens e desvantagens da terceirização de recursos humanos no campo da administração pública, na visão dos usuários desses serviços. Utiliza-se como método de abordagem do problema a pesquisa qualitativa, exploratória. Teve início com a pesquisa bibliográfica e documental, em dados secundários, e utilizando-se dados quantitativos finalizou realizando um estudo de caso com público usuário dos serviços de saúde e com profissionais de educação. Concluindo-se que a terceirização é necessária, porém trouxe mais desvantagem do que vantagem.
Estabilidade no Serviço Público: um estudo de caso aa Autarquia Educacional do Vale do São Francisco
Authors: Amorim Ícaro Teixeira , Oliveira Ricardo Alves de , Lima Maria do Socorro Macêdo Coêlho
Published: October 2016

Este artigo pretende avaliar a importância da estabilidade para os servidores da Autarquia Educacional do Vale do São Francisco – AEVSF, Mantenedora da Faculdade de ciências Aplicadas e Sociais da Petrolina – FACAPE. Buscou-se com o resultado desta averiguação saber qual o ganho trazido para o servidor e para a sociedade. O estudo também tem o intuito de descrever a estabilidade do servidor público nos seus diversos conceitos, tanto doutrinário como legal. A metodologia utilizada neste trabalho foi de cunho descritivo, baseada em estudo de caso, onde foram entrevistados os servidores efetivos da FACAPE.
Avaliação da Aprendizagem: Um desafio a Gestão Escolar
Authors: Silva Eloana Gomes , Oliveira Francisco Das Chagas Rodrigues
Published: October 2016

O artigo propôs uma discussão sobre a importância da avaliação da aprendizagem no processo educacional. Buscou-se encontrar respostas para conflitos entre o ato de avaliar e sua importância na aprendizagem dos alunos. Para isso, esta pesquisa baseou-se em vários autores que apreciam em suas obras uma reflexão acerca dos instrumentos avaliativos usados no contexto escolar, bem como o papel e uso da avaliação no processo ensino-aprendizagem. Observou-se diante das pesquisas que a avaliação não pode ser usada como forma de punir e exercer poder, tornando-se necessária a intensificação das relações entre professor x aluno, principalmente em termos metodológicos. Utilizou-se como método de análise prática a realização de entrevistas e aplicação de questionários para professores a alunos de uma Escola da rede Estadual de Ensino do município de Araripina – PE. Compreende-se então que aprimoramento metodológico, avaliação e aprendizagem, por conseguinte, são aspectos indissociáveis, intimamente ligados e influenciados pela socialização.
Síntese das Contribuições Teóricas da Educação sobre o Processo Ensino-Aprendizagem
Authors: Santos Ronaldo Bezerra dos
Published: October 2016

O presente artigo apresenta de forma resumida, uma coletânea de alguns dos mais conceituados teóricos da educação cujos estudos, pesquisas, observações, tornaram-se relevantes para o processo ensino-aprendizagem, inclusive alguns provocando verdadeiras revoluções na área da educação, imprimindo um novo significado ao contexto educacional em diferentes momentos da história.
Psicoterapia analítico-comportamental em grupo
Authors: Feitosa José Kleidir De Castro , Feitosa Ana Nery De Castro , Oliveira Adriano Monteiro de
Published: October 2016

A necessidade de um procedimento psicoterapêutico em grupo cresce à medida que aumenta também a demanda para atendimentos psicoterapêuticos. O objetivo deste trabalho é explanar sobre aspectos da psicoterapia de grupo sob a ótica da análise do comportamento. Observa-se que o trabalho em grupo pode atingir uma parte da sociedade que não tem acesso ao serviço individual.
Qualidade de Vida, Sobrepeso e Alterações Posturais, como referência para prescrição de Programas de Exercícios Físicos
Authors: Maciel João Paulo Da Silva , Souza Thiago Cabral de , Dias Raphael Rodrigues , Silva Elizabeth Alves
Published: October 2016

O presente estudo, objetiva avaliar a qualidade de vida, prevalência de sobrepeso e alterações posturais, para prescrição do programa de exercícios físico em academias de ginástica no município de Barbalha no estado do Ceara. Trata-se de uma pesquisa, quantitativa, descritiva, de campo e de cunho transversal. A população foi constituída por indivíduos iniciantes de um programa de atividade física em academias de ginástica. A amostra foi composta por 150 indivíduos, sendo 75 homens e 75 mulheres. Verificou-se que a maioria dos indivíduos apresentou alterações morfológicas nos segmentos corporais, observou-se que o principal objetivo da prática é o fator estético e pouco se trabalha a reorganização muscular. Concluir que: não se observou valores de correlações que indicassem associações entre as alterações, que se apresentam inversamente proporcionais, estes quando analisados por gênero, tanto o globoso quanto o protuso são mais encontrados no masculino.
As Políticas Sociais no contexto da reprodução da força de trabalho na dinâmica do capital
Authors: Lima Antônia Ângela de , Beserra Theóphilo Michel Álvares Cabral
Published: October 2016

A investigação ora apresentada teve por objetivo analisar as possibilidades que as Políticas Sociais oferecem na geração de emprego e renda através da qualificação profissional no contexto da reprodução do capital. A presente pesquisa caracterizou-se como descritiva bibliográfica, com abordagem qualitativa, embasado no método crítico dialético. O seguinte trabalho está estruturado em relevantes indagações, onde o primeiro abordará as relações sociais frente ao conflito capital e trabalho perpassando o diálogo sobre a reestruturação e flexibilização das condições de trabalho na contemporaneidade, seguindo a discussão com relação ao surgimento das primeiras escolas de qualificação profissional no Brasil, finalizando com uma breve contextualização das políticas de ensino profissionalizantes a partir da década de 1990 no Brasil.
Avaliação Docente em Questão: tendências e estratégias
Authors: Gonçalves Ana Cristina Oliveira Leite , Oliveira Raimunda Tania Pinheiro de
Published: October 2016

Esse artigo tem por objetivo conhecer a política de avaliação docente, as leis que a regulamentam, os critérios utilizados para sua implantação e as implicações sobre os docentes e a carreira em si. Por meio de uma pesquisa bibliográfica acerca do tema, fundamentado em autores como: Mosé (2015), Gatti (2013), Bauer (2013), Danielson (2011) entre outros. O texto apresenta uma análise do que é avaliar, contextualizando com a avaliação docente, dentro dos propósitos somativos e formativos utilizados no processo avaliativo, na sequência, discute as propostas para a avaliação docente no Brasil, analisando documentos nacionais para identificar as estratégias e objetivos propostos.
CRISPR Explorer: A fast and intuitive tool for designing guide RNA for genome editing
Authors: Chen Kenian , Jin Yi , C. Lin Yin
Published: October 2016

The RNA-guided CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeat-CRISPR-associated 9) system has become a revolutionary technology for targeted genome engineering. The critical step of this technology requires the design of a highly specific and efficient guide RNA (gRNA) that will guide the Cas9 nuclease to the complementary DNA target sequence. CRISPR-Explorer is a new and user-friendly web server for selecting optimal CRISPR sites. It implements the latest scoring schemes of gRNA specificity and efficiency based on published empirical studies. The gRNA design results are generated instantly, thus removing wait times. The user can visualize the high-quality gRNAs with detailed design information through an interactive genome browser. Furthermore, the user can define and specify the parameters for gRNA selection in the Batch Design mode, which recognizes various input formats. CRISPR Explorer is freely accessible at: http://crisprexplorer.org.
RANK/RANKL Expression Is Induced by Cardiac Surgical Operation
Authors: Krejsek Jan , Koláčková Martina , Lonský Vladimír , Trojáčková Kudlová Manuela , Manďák Jiří , Kuneš Pavel , Jankovičová Karolína , Vlášková Dana , Andrýs Ctirad
Published: October 2016

Background: Cardiac surgery provokes a systemic inflammatory response in any patient. This complex body reaction involves also RANK/RANKL molecules which have been recently identified as principal regulators of bone metabolism. Aims: To follow the changes in the expression of RANK/RANKL molecules on innate immune cells of cardiac surgical patients. Patients and Methods: Twenty-six patients undergoing cardiac surgical were assigned to undergo coronary artery bypass grafting using either cardiopulmonary bypass (“on-pump”) or modified “miniinvasive on-pump”. The expression of RANK/RANKL was performed by flow cytometry. Results: Significantly increased expression of RANK on monocytes of “miniinvasive on-pump” patients was found at the 1st, the 3nd, and 7th postoperative days. The similar pattern was found also for monocyte RANKL expression. In addition, RANKL expression was significantly increased at the 3rd postoperative day in “on-pump” patient. No significant differences between “miniinvasive on-pump” and “on-pump” cardiac surgical patients were found. Conclusion: The expression of both RANK and RANKL molecules is significantly enhanced on monocytes of “miniinvasive on-pump” cardiac surgical patients.
“It's Your Face That Is Carrying You Through!”
Authors: Mayhall Laura E. Nym
Published: October 2016

While Nancy Astor's 1919 victory at the polls—making her the first female member of British Parliament—figures prominently in narratives of women's political progress in Britain, the taunt thrown at her while she was campaigning at the Barbican earlier that year, “It's your face that is carrying you through!” figures nowhere in discussions of women's entry into formal political life there. Astor's rejoinder, “No, it's the heart behind it,” points to a tension in her candidacy and subsequent political career that is characteristic of modern celebrity: between the superficial and the genuine, the artificial and the authentic. This text describes how a “film star” and “a personality,” rarely seen by contemporaries as a politician in any masculine sense, successfully publicized the democratic elements of her persona in order to make privilege more palatable in the age of universal suffrage.
Marker-Based Estimates Reveal Significant Nonadditive Effects in Clonally Propagated Cassava (Manihot esculenta): Implications for the Prediction of Total Genetic Value and the Selection of Varieties
Authors: Wolfe Marnin D , Kulakow Peter , Rabbi Ismail Y , Jannink Jean-Luc
Published: October 2016

In clonally propagated crops, nonadditive genetic effects can be effectively exploited by the identification of superior genetic individuals as varieties. Cassava (Manihot esculenta Crantz) is a clonally propagated staple food crop that feeds hundreds of millions. We quantified the amount and nature of nonadditive genetic variation for three key traits in a breeding population of cassava from sub-Saharan Africa using additive and nonadditive genome-wide marker-based relationship matrices. We then assessed the accuracy of genomic prediction for total (additive plus nonadditive) genetic value. We confirmed previous findings based on diallel crosses that nonadditive genetic variation is significant for key cassava traits. Specifically, we found that dominance is particularly important for root yield and epistasis contributes strongly to variation in cassava mosaic disease (CMD) resistance. Further, we showed that total genetic value predicted observed phenotypes more accurately than additive only models for root yield but not for dry matter content, which is mostly additive or for CMD resistance, which has high narrow-sense heritability. We address the implication of these results for cassava breeding and put our work in the context of previous results in cassava, and other plant and animal species.
Male-Specific Transcription Factor Occupancy Alone Does Not Account for Differential Methylation at Imprinted Genes in themouseGerm Cell Lineage
Authors: Romasko Edward J , Engel Nora
Published: October 2016

Genomic imprinting is an epigenetic mechanism that affects a subset of mammalian genes, resulting in monoallelic expression depending on the parental origin of the alleles. Imprinted regions contain regulatory elements that are methylated in the gametes in a sex-specific manner (differentially methylated regions; DMRs). DMRs are present at nonimprinted loci as well, but whereas most regions are equalized after fertilization, methylation at imprinted regions maintains asymmetry. We tested the hypothesis that paternally unmethylated DMRs are occupied by transcription factors (TFs) present during male gametogenesis. Meta-analysis of mouse RNA data to identify DNA-binding proteins expressed in male gametes and motif enrichment analysis of active promoters yielded a list of candidate TFs. We then asked whether imprinted or nonimprinted paternally unmethylated DMRs harbored motifs for these TFs, and found many shared motifs between the two groups. However, DMRs that are methylated in the male germ cells also share motifs with DMRs that remain unmethylated. There are recognition sequences exclusive to the unmethylated DMRs, whether imprinted or not, that correspond with cell-cycle regulators, such as p53. Thus, at least with the current available data, our results indicate a complex scenario in which TF occupancy alone is not likely to play a role in protecting unmethylated DMRs, at least during male gametogenesis. Rather, the epigenetic features of DMRs, regulatory sequences other than DMRs, and the role of DNA-binding proteins capable of endowing sequence specificity to DNA-methylating enzymes are feasible mechanisms and further investigation is needed to answer this question.
КОНТРОЛЬ ЯКОСТІ РИБИ ЗА ЗАСТОСУВАННЯ ВДОСКОНАЛЕНОГО МЕТОДУ ВИЗНАЧЕННЯ ВОЛОГОУТРИМУЮЧОЇ ЗДАТНОСТІ М’ЯСА
Authors: Bogatko N.M. , Bukalova N.V. , Bogatko L.M. , Salata V.Z. , Аrtemenko L.P. , Poltavchenco T.V.
Published: October 2016

Developed an improved method for the determination of water-holding capacity of fish meat by extracting water from a sample of fish meat by the methods of pressing and drying at a temperature of 105 – 106 °C and determine its mass fraction and less in percent by the formula, which will ensure the reliability of results in determining the quality of the fish.Developed an improved method for determining water-holding capacity of fish meat has a probability in the performance 99.4% and can be used to determine the quality of fish at different degrees of heat treatment: fresh, chilled, ice cream, defrosting, cooked ice cream in the determination of its quality in the production laboratories at the facilities of fish processing enterprises on the implementation and storage of fish (stores, supermarkets, wholesale bases, etc.), in the state laboratories of veterinary medicine and in laboratories of veterinary-sanitary examination of agricultural markets in combination with other methods of determining the quality of the fish.It is established that the reliability of the indicators of water-holding capacity of fish meat in comparison of the determination of the mass fraction of water in fish meat was 97.8−98.9% of and before the performance determination of the mass fraction of fat in the fish flesh – 97.9 – 99.0%.Established by research that of water-holding capacity of fish meat depends on the heat treatment of fish, fish species, the mass fraction of water and fat. So, of water-holding capacity he highest ability was observed in meat of fresh fish carp of 72.6 ± 1.7%, the lowest in damastown (thawed) fish perch 51,3 ± 1,6%. For this development Patents of Ukraine is got on an useful model № 109387.
МОРФОЛОГІЧНІ ОСОБЛИВОСТІ МІОКАРДІАЛЬНОЇ ФОРМИ ПАРВОВІРУСНОЇ ІНФЕКЦІЇ СОБАК
Authors: Borysevych B. , Lisova V. , Shats’ka A.
Published: October 2016

Presented results of studying of morphological features of the pathological process at the cardial form of parvovirus infection of dogs. Identified the main criteria for it post–mortem diagnostics.It is shown that based on the results of pathological studies myocarditis of the parvovirus etiology clearly differentiate from other deadly diseases that affect puppies.In puppies aged 1 to 4 weeks, who died suddenly without clinical signs and biochemical changes of blood, which are characteristic for myocarditis, was detected pathomorphological signs of myocardial forms of parvovirus infection of dogs.Histological examination revealed the characteristic morphological changes: 1) lymphocytic (purulent) myocarditis 2) eosinophilic inclusion–bodies in nuclei of cardiomyocytes 3) stromal edema 4) granular dystrophy of cardiomyocytes 5) necrosis of cardiomyocytes with the destruction of the muscle fibers of the myocardium.Also nonspecific but constant morphological features, that is caused by circulatory disorders and heart failure was venous hyperemia of the liver, kidney and pulmonary edema.
ВІДНОСНІ ПОКАЗНИКИ ІМУНОГРАМ У СЕРОПОЗИТИВНИХ НА ТОКСОПЛАЗМЕНУ ІНВАЗІЮ СОБАК ТА КОТІВ
Authors: Broshkov M.M. , Trokosi D.S. , Sheviakova A.A.
Published: October 2016

In most parasitic diseases, the main element of protection of an organism is cell-mediated immunity. His immunological reactivity depends on the ability of an organism to respond adequately to the introduction of antigen. Evaluation of relative indices of the immunogram of HIV-positive dogs and cats have shown that a more pronounced decrease in the relative number of lymphocytes in the experimental group were observed in dogs (33% of the trial) compared with cats (22%). This species difference is probably due to the development cycle of the pathogen and the fact, namely, dogs are the ultimate owners unlike cats. Comparing the relative number of T-helper lymphocytes, it should be noted that seropositive dogs, the figure was higher (by 15% in comparison with the control) and in the middle pozitivnih cats on the contrary marked reduction (47% compared to control). In experimental dogs, compared with control the ability of neutrophils to phagocytosis is less than 8% and cats the difference is 1.5%.When the relative amount by B-lymphocytes in seropositive dogs and cats found that subpopulation of lymphocytes, unlike T-cells, in contrast was higher in the experimental group of animals. In the control group of dogs and cats of these cells is within 11%. In the experimental group of cats increased number of these cells is more pronounced and 3.25% more than in the experimental group of dogs. Analysis of the relative amount of lymphocytes NK- showed that the number of these cells is lower in seropositive for toxoplasmosis dogs and cats. A marked reduction of cell found in cats experimental group (by 5.25%), but in dogs by 1.75%.Given the slight percentage decrease ability neutrophil phagocytosis can be assumed that this cell population is not exposed to marked reduction due to specific receptors vidsutnistyu toksoplazmenoho antigen and it does not allow pathogens directly affect the functional activity of these cells.
THERAPEUTIC EFFECTIVENESS OF THE DRUG RBS – DOG AS IMMUNE MODULATING MEANS IN THE TREATMENT OF DOGS WITH WOUNDS AT HYPO ERGIC TYPE OF INFLAMMATION
Authors: Gajduk M.B. , Gutyj B.V. , Gufrij D.F.
Published: October 2016

The article deals with the results of searches on the therapeutic effectiveness of RBS – DOG (Manufacturer PP «Laboratory Erbis» at Ltd. «Erbis» Kyiv, Ukraine) as immune modulating means in the complex treatment of open mechanical damage in dogs with hypo ergic type of inflammation. The effectiveness of treatment was assessed by clinical condition, speeds wound healing, and also by the results of laboratory tests on blood by morphological and biochemical parameters. It was established that more active recovery of clinical status, wound healing and normalization of dogs blood indices was in the experimental group when using in the complex  treatment of preparation RBS – DOG.Thus, the combined treatment of dogs with purulent wounds using immune stimulating preparation «Erbisol»  and a solution of aqua chelates nanoclusters Argentum and Cuprum along with the acceleration of wound healing leads to a rapid normalization of the morphological structure of blood, compared with the traditional method of treatment.The additional use of the preparation RBS – DOG in doses and by the scheme provided for postcard–tab, improved clinical status, accelerated wound healing, increased immunity, and  speed up the recovery of animals compared to animals that did not receive the drug.In addition to the therapeutic effect, the preparation RBS – DOG was well tolerated target by animals and caused no side effects.
ВПЛИВ ФУМОНІЗИНОТОКСИКОЗУ НА МОРФОЛОГІЧНІ ТА БІОХІМІЧНІ ПОКАЗНИКИ КРОВІ ЩУРІВ
Authors: Guta Z.
Published: October 2016

The article deals with the influence of fumonisins on morphological and biochemical indices of rats blood. 20 rats weighing 165-170 g were used in the experiment. It was formed two groups: first group of animals - served as a control, in the second experimental group – chronic fumonisin toxicosis  was reproduced. Rats were daily injected with intra- gastro intestinal 90 mg of fumonisin per one animal. It was established that after the introduction of fumonisin to rats, mycotoxins have a negative impact on the morphological indices of rats blood. It wass found a probable increase in the number of leukocytes to 18.9 g/l, the number of eosinophils to 6.7% segmented neutrophils to 30.7%, and also the decrease in the number of lymphocytes and monocytees to 56.0 and 0.7%. In the analysis of leukocyte formula we have noted the tendency to displacement the nucleus left.Reactions that occur on the background of  the above mentioned toxicity,caused by selective tropism for various tissues of the body; as a result there are nefro-, hepato- and nephrotoxic reactions.To diagnose these changes is possible  after a comprehensive study on the basis of biochemical changes. At research of biochemical parameters in rats blood by the development of chronic fumonisin toxicosis  it was set the reduction of  the level of total protein; indicating the development of violations of proteins exchange in rats organism and protein synthesized liver function.Also it was found that under the conditions of  fumonisin toxicosis  in rats, alanine -aminotransferase activity and aspartate- aminotransferase in their blood throughout the experiment was increased.This is explained by increased permeability of cells under the influence of fumonisin that influenced directly on the membrane, probably, disrupting their structural components. The concentration of creatinine and urea in the blood of experimental rats which were  fed with fumonisin, exceeded the physiological values that were clinical sign of the development of inflammation in rats organism.On the 14th  day the phagocytic activity of neutrophils and phagocytic index of rat blood affected by fumonisin was reduced, indicating the availability of such changes in the immune system of animals.
INFLUENCE OF «METISEVIT» ON THE ACTIVITY OF ENZYME AND NONENZYME LINK OF ANTIOXIDANT PROTECTION UNDER THE BULL’S BODY CADMIUM LOADING
Authors: Gutyj B.V. , Lavryshyn Y. , Binkevych V. , Binkevych O. , Paladischuk О. , Strons'kyj J. , Hariv I.
Published: October 2016

The article contains the research results of the effect of cadmium chloride on the indexes of enzyme and nonenzyme systems of  antioxidant defense system in young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E. It is established that feeding calves at a dose of toxicant 0.04 mg / kg activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E in the blood of experimental animals decreased throughout the experiment. The lowest indicators of antioxidant in the blood of young cattle is set on the twenty -fourth day of the experiment, which is associated with increased activation of lipid peroxidation and the balance between antioxidant system and lipid peroxidation intensity. Given the cadmium load of young cattle it is used a new integrated drug with antioxidant action «Metisevit», which includes metifen, sodium selenite and vitamin E wich is founded as stimulating effects on the activity of antioxidant protection. In particular,it is established probable increase in activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, vitamin A and vitamin E in the blood of young cattle, which has performed cadmium stress. These changes occur through comprehensive action components of the drug «Metisevit» that leads to the normalization of metabolic processes and free radical in the body of the bull. The results of the research indicate antioxidant drug «Metisevit» in the application of its young cattle and the validity of his administration to improve the body's antioxidant status of chronic cadmium toxicosis.
МОРФОЛОГІЧНА ХАРАКТЕРИСТИКА ВНУТРІШНІХ ОРГАНІВ ІНДИКІВ ПРИ ЗАСТОСУВАННІ ПРЕПАРАТУ «БІОТОН»
Authors: Zhyla М. , Shkil M. , Ponomarenko S.
Published: October 2016

In the article the results of clinical trials are given in relation to an unconcern and efficiency of new veterinary preparation Bioton, to solution for per oral application, that contains complex biologically active foods of metabolism of mushrooms-endophytes (Cylindrocarpon Magnusianum), medical plants abstracted from a root. For the study of application of veterinary preparation Bioton it was formed 3 groups of turkeys of cross «Big-6», for 25 heads in each. The first group got preparation in a dose a 1 ml/l, the second group got in a dose 2 ml/l of water during 7day in 1, 6, 15, 21 week to fattening, the third group did not get preparation – control. The dynamics of change of body weight was determined by the individual weekly weighing. In times of carrying out the test of rejections in relation to behavior, clinical state of turkeys and diseases did not appear. Preparation Bioton, solution for per oral application, well carried by turkeys, did not cause structural changes in the investigated internals, assisted the improvement of metabolic processes, increase of body and for slaughter exit of carcass weight. Macroscopic of internal organs of turkeys that got preparation Bioton, in a dose 1–2 ml/l of water during 7 days in 1, 6, 15, 21 week of fattening, kept a characteristic anatomic structure. Weight coefficients of thymus, bursa of Fabricius, of testicles were higher in turkeys of the second group. The histological structure of the investigated internal organs specified on their active morphofunctional state in accordance with age of turkeys. The difference of indexes was more expressed in an experience group that got preparation Bioton in a dose 2 ml/l of water. Then, as for the turkeys of control group, we found out the changes of dystrophic-necrotizing character in a liver and atrophy of lymphoid tissue of organs the immune system.
ЗАХОДИ БОРОТЬБИ З АСОЦІЙОВАНИМ ПРОЯВОМ ПАСТЕРЕЛЬОЗУ, СТАФІЛОКОКОЗУ ТА ВІРУСНОГО СТОМАТИТУ КРОЛІВ В УМОВАХ ПРИВАТНОГО ГОСПОДАРСТВА ПОЛТАВСЬКОГО РАЙОНУ
Authors: Peredera O.O. , Lavrinenko I.V. , Zhernosik I.A.
Published: October 2016

In the article epizootological data, clinical signs, pathological changes and laboratory research associate for the manifestation of pasteurellosis, stafilokokos and viral stomatitis of rabbits. The work was performed in 2016 in the private sector Poltava region. Diagnostics performed comprehensively, taking into account symptoms and results of microscopic and bacteriological research.The disease is filed within three rabbits. Animals lost weight, felt very thirsty, became depressed, inactive. Appetite is usually in early disease did not change, but with the development of clinical signs deteriorated. Developed rhinitis, first serous then mucopurulent. In disorders of respiratory function observed dysfunction of the gastrointestinal tract - diarrhea alternating with constipation, the abdomen becomes soft, not firm. The main clinical feature of this period was a progressive dehydration: dry and anemichnist visible mucous membranes, eyes retraction deep into orbit. The back of the body was contaminated liquid stool consistency. Fur becomes darker, hair becomes disheveled, easily removed from hair follicles. In the corners of the eyes accumulated significant amount of muco-purulent fluid, which in some cases led to a adhesive forever. Rabbits died on the background of progressive weakness.Autopsy of the dead showed signs of sero-hemorrhagic or sero-fibrinous pneumonia, sero-hemorrhagic pleurisy and peritonitis. Recorded splenit in bowel - wall thickening, signs of acute catarrhal enteritis, multiple hemorrhages in the mucosa and signs of venous congestion.In developing control measures take into account the existence of the association. In this economy together with the associated pasteurellosis was diagnosed eymeriosis because besides antibacterial substances it was necessary to use eymeriostatyks. Dedicated pathogens of stafilokokosis and pasteurellosis showed sensitivity to enrofloxacin and tylosin. Animals of one group was used brovaseptol complex preparation, the drug used in another 50% of tylosin and salikoks. The high therapeutic effect provided the use of biologics in animals of both groups. The use of tylosin were effective individually in combination with solikoks and the application of complex drug brovaseptol. A week after recovery animals were immunized rabbits all vaccine Pazorin-Ol, (Pasorin-Ol).
ДІАГНОСТИКА ТА ЗАХОДИ БОРОТЬБИ З ПАСТЕРЕЛЬОЗОМ ГУСЕЙ В ПРИВАТНОМУ ЗООПАРКУ ПОЛТАВСЬКОЇ ОБЛАСТІ
Authors: Peredera O.O. , Lavrinenko I.V. , Zhernosik I.A.
Published: October 2016

The results of the study geese pasteurellosis outbreaks in private zoo in the Poltava region. Diagnosis for pasteurellosis set comprehensively, taking into account epizootological features, clinical signs and pathological changes.The diagnosis was carried out comprehensively, taking into account the characteristics of epizootic, clinical signs and postmortem autopsy results. In determining the diagnosis were the results of microscopic and bacteriological research. To study the morphology of microorganisms strokes, the selected cultures were stained with alcoholic–aqueous methylene blue. We studied the sensitivity of selected microorganisms to certain antibiotics. After 24 hours of incubation in nutrient medium grew rounded, smooth, «wax» colonies of microorganisms.In smears of colonies that grew were found to IPA Pasteurella – typical bipolar coli. After obtaining a pure culture was carried determine the sensitivity of the selected pathogen to various antibacterial agents. For this we used disco–diffusion method. After the incubation, the Petri dish looked and measured in millimeters zone delay line growth of microorganisms.According to the results obtained, isolated Pasteurella culture revealed high sensitivity to tilozyn. This antibacterial agent caused stunted growth zone microflora 14–21 mm; High sensitivity also highlighted the culture was determined to enrofloxacin (zone of stunted growth – 16–24 mm) and gentamicin (zone of stunted growth – 18–24 mm) Moderately resistant culture was to kanamycin and chloramphenicol (zone growth retardation – 8–14 mm in accordance). To the rest of the drugs was highlighted microflora resistant, zone microflora growth delay ranged from 0 to 4 mm.The drug Enroksil 10% solution, based on enrofloxacin used orally in drinking water at the rate of 5 ml per 10 liters of water for three days. During treatment birds received only water containing the drug. The high sensitivity of microflora selected a number of antibacterial drugs and high efficiency of therapeutic measures can be explained by timely treatment zoo owner for help with diagnosis.
КОМПЛЕКСНІ ПРЕПАРАТИ НА ОСНОВІ НАНОБІОМАТЕРІАЛІВ, ПЕРСПЕКТИВИ ВИКОРИСТАННЯ У РЕПРОДУКЦІЇ ОВЕЦЬ ТА КІЗ
Authors: Sklyarov P.M. , Koshevoy V.P.
Published: October 2016

A complex preparations «Kahadin + OV», «Kaplaestrol + OV», «Karafest + OV» and «Karafand + OV», which have antioxidant, anti-hypoxic and membranoprotective properties thanks to nanobiomaterials.Were tested drug «Karafest + OV Zn», at doses of justification which came out of the daily needs of animals in the components of the drug, which for sheep and goats in the last 7-8 weeks of pregnancy (per animal), carotene – 0.28–0.5 mg/kg, or 12–26 mg, phytoestrogens – 0,0175 mg/kg or 0.61-1.22 mg, zinc – 1-1.08 mg/kg, or 54-70 mg.The content of substances in 1 ml «Karafest + OV Zn» according to the technical specifications are: carotene - 10 mg (1,2-2,6 ml), phytoestrogens - 1 mg (0.6-1.2 ml), zinc carbonate – 2 mg, ortovanadatе europium activated gadolinium – 0.00015 mg.The final dosage was determined by the scheme – 0.35–2.1 ml/animal оr 0.01–0.03 ml/kg body weight/day.The drug «Karafest + OV Zn» asked orally with food daily for a month to projected pregnancy (140 days of pregnancy).The positive effect of the drug «Karafest + OV Zn» at the homeostasis parameters in sheep, including normalization of serum vitamin A, zinc and total protein; prooxidative-antioxidant system containing serum – malondialdehyde, catalase, superoxide dismutase and erythrocyte – malondialdehyde, catalase, restored glutathione and oxidative-antioxidant value;  system state  oxygen  metabolism  –  the number of red blood cells and hemoglobin concentration of 2.3-dyphosphoglyceratis.Thus, the use of the drug «Karafest + OV Zn» increased content in blood serum, vitamin A (by 16.2%), zinc (by 23,4%), total protein (by 11.5%), malondialdehyde (by 17.5%), catalase (by 24.7%), superoxide dismutase (by 17.9%); in erythrocytes: (by 24.4%), catalase (by 23.9 %) and reduced glutathione (by 18.2%) and hemoglobin (by 23.5 %), the actual number of red blood cells (by 21.8%), the concentration of 2,3-dyphosphoglyceratis (by 22.2%) and normalized prooxidant-antioxidant ratio.
ТОКСИКОЛОГІЧНА ОЦІНКА ПРЕПАРАТУ «БІОКОНТАКТ ПЛЮС» ДЛЯ МЕДОНОСНОЇ БДЖОЛИ
Authors: Tushak S.F. , Romanishina T.O.
Published: October 2016

The article is devoted to the toxicological estimation of the disinfectant of new generation «Biocontact plus» for bees using the method of contact and oral toxicity definition. The experiments were carried out at the laboratory of Zhytomyr National Agroecological University at the faculty of Veterinary Medicine, Department of Microbiology, Pharmacology and Epizootology. Definition of contact and oral toxicity was carried out by the method of bees’ group treatment in entomophilic gardens and isolators. The duration of the observation period: for acute phase – 48 hour, for chronic phase – 20 days. It was concluded, that the contact use of the drug results in temporary effect, if concentration is 0.3%. While estimating the toxicity in the stomach, the effect of acute toxicity was observed at the level of concentration 0.3%. If the drug is used with the concentration 0.1% and 0.15%, is has stimulating and activating effect. If the level of concentration is low (0.03%, 0.01%), no positive changes in the vital functions of the bees were observed. The possibility of use the drug in the function of stimulator for bees using the method of nutrition additives with the level of concentration 0.1% and 0.15% was proved.It is necessary to study the possibility of the usage of the drug in the function of stimulator for bees using the method of nutrition additives with the level of concentration 0.1% and 0.15% taking into consideration the changes of physiological markers (cytological and morphological hemolymph composition, development of lipid droplet), assessment of influence on the economically useful markers of families, changes in sanitary and hygiene behaviour of bees.
ЗБЕРЕЖЕННЯ ЖИТТЄЗДАТНОСТІ ЯЄЦЬ ТА ЛИЧИНОК СТРОНГIЛЯТ I АСКАРИДАТ У ДОВКІЛЛІ В ЗИМОВИЙ ПЕРІОД
Authors: Feshenko D.V. , Bakhur T.I. , Zgozinska O.A.
Published: October 2016

This paper presents the results of the helminthological study of sand, hay and silage in different periods of 2010 – 2015. It was established that on the territory of Polissya the eggs and larvae of nematodes (Parascaris equorum, Strongylidae sp., Toxocara canis) at typical winter air temperature (-15 – +3 °C) remain viable in the environment, contributing to the spread of continuous year-round animal infestation.We prove that in the stall period of keeping animals rough and succulent feed (hay, silage) can be a source of infection of nematodes even in late winter: according to our data, in February in the hay the number of eggs P. equorum was 2.6 ± 0.74 eggs/3 drops of flotation solution, in the silage – 8.8±1.01, and Strongylidae sp. – 3.2 ± 0.73 and 5.6 ± 0.6 eggs/3 drops of flotation solution respectively. Consequently, for the prevention of helminthiasis all harvested food should be checked for the presence of pathogens in it.Maximum contamination of sand from playgrounds in the Zhytomyr region by toxocara eggs was: 674.6 eggs/3 drops of flotation solution in autumn and 60.3 – in the spring. Totally 12.8–18.4% of T. canis eggs are overwintering in the sand successfully. The data should be considered when planning preventive veterinary measures. Recommended in disadvantaged regions to carry out preventive deworming animals every 3 months.
ПОКАЗНИКИ ПЕРОКСИДНОГО ОКИСНЕННЯ ЛІПІДІВ У КРОВІ ВІВЦЕМАТОК, ХВОРИХ НА ГЕПАТОДИСТРОФІЮ
Authors: Chernushkin B.O. , Slivinska L.G. , Vlizlo V.V.
Published: October 2016

The article deals with the results of studies on the causes and clinical manifestations of hepatodystrophy in ewes, indicators status of lipid peroxidation – TBA-active products, lipid hydroperoxides, diene conjugates.In order to establish the causes of hepatodystrophy in sheep, we have analyzed the feed rations. Sheep investigated 24 local breeds aged 3 – 4 years, weighing 45 – 56 kg. Conducted clinical study animals and blood were collected for laboratory analysis. Serum sheep determined the content of malondialdehyde (MDA-active products) using thiobarbituric acid (Stalnaja I.D., Haryshvyly T.G, 1977) and lipid hydroperoxides using ammonium thiocyanate (Romanova LA, Stalnaja I.D., 1977). In blood examined diene conjugates (Stalnaja I.D., 1977).High performance is ensured animals by creating appropriate technical conditions and feeding. Violation of maintenance processes and non-biological nutrition of the cause performance degradation, the spread of disease and culling of animals. Development hepatodystrophy animals observed in animals fed low-quality forage and depraved that contain a number of toxic products of oxidation of fats – aldehydes, ketones, low molecular weight acids, hydroxy acids, which have toxic properties and also due to an unbalanced diet for the main nutrients and biologically active substancesThe main causes of primary hepatodystrophy in sheep is imbalanced nutrition and lack of essential nutrients in the diet. In analyzing the diet of established lack of dry matter, raw protein, digestible protein, raw fiber, sugar and starch. Value sugar and starch with digestible protein accounted for 1,78: 1, and sugar-protein ratio – 0,77. Rations were scarce for vitamins D and E, microelements – Sulphur, copper, zinc, cobalt and iodine.In ewes suffering from hepatodystrophy was set the development of oxidative stress – increase in blood activity of TBA-active products, lipid hydroperoxides, diene conjugates. In particular, concentration of diene conjugates was increased by twice the number of hydroperoxides lipids was increased by three times and the activity of TBA-active products was increased by five times compared with clinically healthy animals. Histological examination of liver biopsies of ewes was set that the develop of protein granular and fatty degeneration of hepatocytes.
ХАРАКТЕРИСТИКА МОЖЛИВОСТЕЙ ІМУНОФЕРМЕНТНОГО АНАЛІЗУ
Authors: Shemediuk N.
Published: October 2016

Development of biotechnologies in modern world is one of the pressing questions in medicine, pharmacology, agriculture,  ecology progres,  different  spheras of industry  in  particular food. In  fact, development of approaches and facilities of analytical biotechnology with application  of   monoklonal antibodies (MKA) are actual nowdays.Contamination of animal forages by mikotoxins, active using of antiinfectives, growthfactors in a stockraising (that provides raw material for food industry) stipulates thenecessity of introduction  optimal methods of analytical control of  foodstuffs quality. Diagnostics  of man  diseases,  control of the environment state  are other important questions. The МКА, ELISA are ways of decision these questions.Comparatively subzero prime price, short duration time of analysis implementation , an inexpensive equipment explains to perfection, gives an opportunity to apply ELISA-test in  different spheres of activity.
THE CONCENTRATION OF FREE AMINO ACIDS IN BLOOD SERUM OF HEALTHY COWS AND COWS WITH SUBCLINICAL KETOSIS
Authors: Marczuk J. , Brodzki P. , Brodzki A. , Lutnicki K.
Published: October 2016

This paper presents the study on determination of the free amino acid in blood serum of cows with high milk production (Herd A) and cows with subclinical ketosis compared to healthy ones (Herd B).  In Herd A examinated 12 cows in the first 100  days in milk. A total of 24 cows  from a herd B divided into two groups: experimental (12 cows with ketosis) and control (12 healthy cows) were included in the study. Statistically significantly higher concentrations of glutamine, glutamic acid, isoleucine (p ≤ 0.001), and tyrosine (p ≤ 0.05) were found in dairy cows with subclinical ketosis compared to healthy ones. A significant decrease in the concentrations of asparagine, histidine, methionine, and serine (p ≤ 0.001) as well as alanine, leucine, lysine and proline (p ≤ 0.05) was observed. In Herd A was high level of total essential amino acids  in blood serum. In our study, the changes, in particular, observed in amino acid concentration in cows with subclinical  ketosis indicate its intensive use in both ketogenesis and gluconeogenesis processes.