bluuebunny/crossref_metadata_2025 huggingface dataset:
Benefit evaluation of human resource management in agricultural enterprises based on convolutional neural network
Authors: Zhang Ning
Published: May 2023

With the rapid development of economy, the performance appraisal of human resource management in agricultural enterprises has gained more attention. Based on the convolutional neural network theory, this paper constructs the benefit evaluation system of human resource management in agricultural enterprises, determines the weight to obtain the comprehensive benefit result, and then obtains the evaluation level. According to the main factors affecting the human resource benefit of agricultural enterprises, the model designed the input and output indexes of the efficiency evaluation of single well of production capacity construction, and solved the problem of measuring the informatization ability of human resource management in agricultural enterprises. In the simulation process, the convolutional neural network designed the evaluation index of the index system. According to the different contribution and importance degree to the system security, the difference between the evaluation index can be expressed by assigning different weight values. Secondly, for items with similar loads on multiple factors or with low loads on a single factor, it was adopted to obtain a scale consisting of 54 items, including 15 items in interpersonal skills sub-scale, 16 items in learning development sub-scale and 23 items in growth driver sub-scale. The experimental results show that the convolutional neural network training can obtain the reasonable scale value of the test sample management area. The evaluation index of the model prediction effect shows that the absolute relative error, maximum absolute relative error and average absolute relative error are all within 5%, and the equality coefficient is 0.9845, which is greater than 0.9, indicating that the reasonable scale predicted value has a high degree of fitting with the expected value. The prediction results of the model are ideal, which effectively improves the accuracy of benefit evaluation
Toxic effects of essential oils against Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) and its certain important natural enemies
Authors: Sen Emre
Published: May 2023

In this study, we aimed to investigate the effects of five different commercially available essential oils; aloe vera, borage, cacao, horse chestnut and nettle seed oils against egg, nymphal and pupal stages of Bemisia tabaci Gennadius and its important natural enemies namely, Encarsia formosa Gahan, Eretmocerus eremicus Rose and Zolnerowich, Macrolophus pygmaeus Rambur, Nesidiocoris tenuis Reuter, and Orius laevigatus Fiber. This study was conducted under laboratory conditions in 2021 at Bursa Uludag University, Turkey. The essential oil solutions were applied at %0.125, 0.25 and 0.5 doses and mortality rates were noted after 24, 48 and 72 hours of essential oils application. As a result, it was determined that the insect species most affected by the plant oils were the predators, the immature stages (egg, nymphal and pupal) of whitefly and the parasitoid species, respectively. Borage and horse chestnut oils showed high effects (100%) on predatory adults after 24 hours. It was determined that all N. tenuis and M. pygmaeus adults died after 48 hours in the trial. The egg, nymphal and pupal stages of B. tabaci were most affected by plant oils, respectively. B. tabaci eggs and pupal stages were the most affected from cacao oil with a mortality rate of 100%. Moreover, nymphal stage of B. tabaci was the most affected by horse chestnut oil with a mortality rate of 100% after 72 hours. Parasitized pupae were found to be the least affected individuals in the trial. Aloe vera oil was the least effective essential oil in the experiment. Eggs and nymphal stages of B. tabaci, O. laevigatus, E. formosa and E. eremicus were affected by aloe vera oil with mortality rates of 53.33%, 46%, 53%, 46% and 36%, respectively. Horse chestnut and cacao oils were harmful to the immature stages (egg, nymphal and pupal) of B. tabaci and all predator adults (M. pygmaeus, N. tenuis, O. laevigatus), but they had slightly harmful on parasitoid species in the experiment
Expert consensus on evidence-based recommendations for the diagnosis, treatment, and follow-up of X-linked hypophosphatemic rickets (XLH)
Authors: Aguilera Juan Guillermo Cárdenas , Orjuela Adriana Medina , Meza Adriana Isabel , Prieto Juan Carlos , Bahamón Ana María Zarante , Mosquera Jimena Adriana Cáceres , Gaviria Natalia Mejía , Gayubo Ana Katherina Serrano , Rodriguez Richard Baquero , Acevedo Kelly Chacón , Tinoco Gustavo Adolfo Guerrero , Rios Alejandro Uribe , Rueda María Fernanda García , Londoño Verónica Abad , Almanza Sergio Alejandro Nossa , Martínez Gustavo Aroca , González Alejandro Román , Cáceres Jorge Alberto Endo , Linares Juan Pablo Llano , Valdes Pablo Florenzano , Curiel Manuel Diaz , Vaisbich María Helena , Zanchetta María Belen , Hernández Norma Elizabeth Guerra , Stefano Eduardo Enrique , Brunetto Oscar
Published: May 2023

Background:X-linked hypophosphatemic rickets is a hereditary disease that generates alterations in bone mineral homeostasis. The morbidity of the condition has been variable in previous decades and even contradictory, probably due to the definition of the case and the diagnostic confirmation. Our propose was to generate evidence-informed recommendations for the diagnosis, treatment, and follow-up of patients with suspected or diagnosed XLHR.
Results:After the screening and selection process for 1041 documents, 38 were included to answer the questions raised by the developer group. 97 recommendations about the diagnosis, treatment, and follow-up of patients with suspected or diagnosed XLHR were approved by the experts consulted through modified Delphi consensus. The quality of the evidence was low.
Conclusions:The recommendations proposed here will allow early and timely diagnosis of X-linked hypophosphatemic rickets, while optimizing resources for its treatment and follow-up and help clarify the burden of disease and improve health outcomes for this population.
The challenges in diagnosis of multisystem inflammatory syndrome in children (MISC), comparison of MISC and drug eruption: A case report
Authors: chaichy zahra , moazzen nasrin , aelami mohammadhasan , vahedi mahdieh , Amirabadi Amir , khosravi omid , aali amirali , Ahanchian Hamid
Published: May 2023

Unusual manifestations are possible for multi-system inflammatory syndrome brought on by SARS-Cov2 infection. Early diagnosis and effective treatment have a direct impact on the outcome. Every young patient who presents to the clinic with a fever, skin rash, stomach discomfort, or cardiovascular complications has to be evaluated for this potentially fatal disease. It is also of utmost importance to differentiate MIS-C from drug hypersensitivity (DHS). MIS-c highly resembles DHS but leads to more complications and a higher mortality rate. We report a 9-year-old female who initially presented with generalized abdominal pain, nausea, vomiting, and cough. She gradually developed an acute abdomen and was admitted for surgical management of a suspected perforated appendix. Her condition deteriorated despite surgery and medical treatment. Differentiating drug allergy from this new emerging syndrome can be difficult. Herein we discuss about it.
Hijacking cysteine metabolism for reversing hydrogen sulfide production in enhanced ferroptosis therapy
Authors: Xu Xiang , Wang Fang , Luo Rengan , Lei Jianping
Published: May 2023

Intracellular cysteine (Cys) is an essential element in glutathione (GSH) biosynthesis, which endows cancer cell resistance to various therapies. In this study, we design a Cys metabolism hijacking strategy for converting cytoprotective Cys into therapeutic hydrogen sulfide (H2S), thus achieving enhanced ferroptosis therapy. A therapeutic nanoagent is first synthesized via the co-assembly of L-buthionine-sulfoximine (BSO) and plasmid encoding fn1055 with metal-organic framework (MOF) Fenton catalyst. The encapsulated BSO blocks the Cys-GSH biosynthesis pathway while the Fn1055 protein expressed from plasmid catalyzes the conversion of intracellular Cys to H2S, accelerating Cys-H2S metabolism. Due to its triple functionalities of intracellular acidification, catalase inhibition and mitophagy promotion, H2S significantly accelerates the MOF-induced ferroptosis, which is rationalized by the fully shrunken mitochondria. The in vivo administration of nanoagent effectively suppresses xenograft tumor growth without appreciable side effects. Such Cys metabolism hijacking provides a proof of concept for remolding the Cys-GSH pathway as a promising therapeutic strategy in cancer therapy.
Antioxidants for male infertility: therapeutic scheme and indications. A retrospective single-center real-life study
Authors: Cannarella Rossella , Crafa Andrea , Kaiyal Raneen Sawaid , Kuroda Shinnosuke , Barbagallo Federica , Alamo Angela , Mongioì Laura M. , Sapienza Sabrina , Condorelli Rosita A. , Vignera Sandro La , Calogero Aldo E.
Published: May 2023

This single-center real-life study was conducted to evaluate the most effective combination of nutraceuticals and the most appropriate indications for the treatment of male infertile patients. Infertile patients aged 20–55 years were treated with a combination of antioxidants (Androlen®) (Group 1), with Androlen® and a mixture of fibrinolytic molecules (Lenidase®) (Group 2), or Androlen® and other molecules different from those used for the patients of the Group 2 (Group 3). Patients were also subdivided according to the presence of varicocele, mild testicular hypotrophy, idiopathic infertility, and chronic male accessory gland infection (MAGI). Forty-three patients were enrolled. In the overall analysis, only progressive motility significantly improved after therapy. Subgroup analysis showed a significant increase in progressive motility, total motile sperm count (TMSC), and in the percentage of alive spermatozoa after treatment in the Group A. Progressive motility improved significantly in patients with varicocele, while the TMSC in patients with varicocele and those with idiopathic. The percentage of alive spermatozoa increased in patients with testicular hypotrophy. Treatment with antioxidants increases progressive sperm motility. The association of fibrinolytic or other nutraceuticals does not improve the efficacy of the treatment with antioxidants alone. The treatment was effective in patients with varicocele or idiopathic infertility.
Pregnancy experience among West-African migrant women during covid-19 pandemic in France
Authors: LEIMDORFER Chloé , OGRIZEK Anaïs , AZRIA Elie , COMPEYRON Alice , MORO Marie Rose , RADJACK Rahmeth
Published: May 2023

BackgroundThe health crisis led to numerus questions regarding health care measures, psychological experience of lockdown, and health care access inequities according to migration status during pregnancy. This qualitative study explores the subjective experience and cultural representations around pregnancy and mother-child bond among migrant mothers of first and second generation during the Covid-19 pandemic in France.MethodWe conducted semi-structured interviews to collect the experience of migrant mothers from West Africa who had given birth between October 2021 and may 2022 (in between covid’s 4th and 6th wave) in two maternity hospitals in France. We used interpretative phenomenological analysis to explore the data.ResultsNine mothers, first and second generation of migrants from West African countries were included. Three different themes emerged: increased holding capacities from health-workers and family concerning pregnancy issues in the pandemic context; mothers’ wisdom in their description of the pandemic; and increased awareness induced by pandemic context on cultural representations and mental shuffle regarding motherhood.ConclusionMore generally, our research is an invitation to reflect on health care inequalities and how to deal with cultural differences during perinatal period, regardless of the sanitary or political context.
Long-term symptoms after SARS-CoV-2 infection in a cohort of hospital employees: duration and predictive factors
Authors: Gruber Rosalie , Ludewig María Verónica Montilva , Weßels Christina , Schlang Gerlinde , Jedhoff Svenja , Herbrandt Swetlana , Mattner Frauke
Published: May 2023

PurposeTo evaluate the frequency, duration and patterns of long-term coronavirus disease 2019 (COVID-19) symptoms and to analyse risk factors for long-lasting COVID-19 sequelae among a cohort of hospital employees (HEs).MethodsWe conducted a survey regarding persistent COVID-19 related symptoms with all HEs from three medical centres in Cologne, Germany, who were tested SARS-CoV-2 PCR positive from March 2020 until May 2021. Duration of symptoms and possible risk factors for protracted COVID-19 course were analysed.ResultsOf 221 included HEs, a number of 104 HEs (47.1%) reported at least one persisting symptom for more than 90 days after initial SARS-CoV-2 detection. Each one cycle higher initial Ct value significantly increased the chances of overcoming symptoms (odds ratio [OR] = 1.05; p = 0.019). The occurrence of breathlessness within the first ten days (OR = 7.89; p = 0.008), an initial Ct value under 30 (OR = 3.36; p = 0.022) and a definitely nosocomial SARS-CoV-2 transmission (OR = 3.05; p = 0.049) showed a statistically significant association with increased odds of illness duration over 90 days.ConclusionAbout half of the HEs suffered from long lasting symptoms over 90 days after almost entirely mild acute COVID-19. Predictive factors could possibly be used for early treatment to prevent development of long-term symptoms after COVID-19 in future.
CVID-associated intestinal disorders in the USIDNET registry: An analysis of disease manifestations, functional status, comorbidities, and treatment
Authors: Franzblau Lauren E. , Fuleihan Ramsay L. , Cunningham-Rundles Charlotte , Wysocki Christian A.
Published: May 2023

Common variable immunodeficiency (CVID) has been subdivided into five phenotypes, including one marked by non-infectious enteropathies that lead to significant morbidity and mortality. We examined a large national registry of patients with CVID to better characterize this population and understand how the presence of enteropathy influences nutritional status, patient function, and the risk of additional non-infectious disorders in CVID patients. We also sought to illustrate the range of treatment strategies for CVID-associated enteropathies. We extracted patient data from the United States Immunodeficiency Network (USIDNET) database, which included 1415 patients with CVID, and compared those with and without intestinal disorders. Demographic and genetic profiles, functional status, and treatments targeting intestinal disorders are reported. Intestinal disorders were present in 20% of patients with CVID, including chronic diarrhea, inflammatory bowel disease, malabsorption, and others. Compared to those without enteropathies, this patient subset exhibited significantly lower Karnofsky-Lansky functional scores, greater reliance on nutritional support, higher rates of vitamin deficiencies, and increased prevalence of hematologic disorders, liver disease, pulmonary disease, granulomatous disease, and lymphoma. Genetic data were reported for only 5% of the cohort. No mutations segregated significantly to patients with or without intestinal disease. Corticosteroids were most frequently used for treatment. Patients with CVID-associated intestinal disorders exhibit higher rates of autoimmune and inflammatory comorbidities, lymphoma, malnutrition, and debility. We review recent studies implicating specific pathways underlying this immune dysregulation. Further studies are needed to evaluate the role of targeted immunomodulatory therapies for CVID-associated intestinal disorders.
Construction of a F 1 DH population and high-density genetic map of ornamental kale
Authors: Guo Ning , Han Shuo , Zong Mei , Wang Guixiang , Duan Mengmeng , Liu Fan
Published: May 2023

Background Ornamental kale (Brassica oleracea var. acephala) is an attractive ornamental plant with a range of leaf colors and shapes. Owing to the long breeding cycle and limited availability of genetic markers, the breeding of new varieties of ornamental kale by traditional and marker-assisted selection (MAS) breeding methods is difficult. Microspore culture is an effective approach to generate new materials and a doubled-haploid (DH) population, which is suitable for genetic map construction. Genetic maps are a prerequisite for quantitative trait loci analysis, MAS, fine gene mapping, and genome sequence assembly. Here, we report the construction of a F1-derived doubled haploid (F1DH) population and high-density genetic map for ornamental kale to provide novel resources to use in breeding new varieties.Results A F1DH ornamental kale population comprising 300 DH lines, derived from parents that differed markedly in leaf shape and color, was constructed using microspore culture. More than 1000 regenerated plants were obtained, showing that the F1 progeny possessed high regeneration capacity, and the proportion of spontaneous chromosome doubling was higher than 50%. The F1DH population contained rich phenotypic diversity, especially variation in leaf color and shape. A high-density genetic map was generated by whole-genome resequencing using 150 F1DH individuals selected from the F1DH population. The genetic map contained 1696 bin-markers with 982,642 single-nucleotide polymorphisms (SNPs) spanning a total distance of 775.81 cM on all nine chromosomes with an average distance between markers of 0.46 cM. The ornamental kale genetic map contained substantially more SNP markers compared with published genetic maps for other B. oleracea crops. The haplotype and heat maps as well as the collinearity of the genetic and physical maps illustrated that the genetic map for ornamental kale was accurate and of high quality.Conclusions The F1DH progenies provide an excellent resource for germplasm innovation and breeding new varieties of ornamental kale. The high-density genetic map provides useful information for gene mapping and elucidation of the molecular mechanism of important agronomic traits of ornamental kale, and will aid in pseudochromosome assembly in genomic research on B. oleracea.
Derlin-1 ameliorates nonalcoholic hepatic steatosis by promoting ubiquitylation and degradation of FABP1
Authors: You Hui , Wen Xin , Wang Xingchun , Zhu Cuiling , Chen Haibing , Bu Le , Zhang Jun , Qu Shen
Published: May 2023

BackgroundThe functions of liver fatty acid binding protein (FABP1) in the regulation of nonalcoholic fatty liver disease (NAFLD) have been demonstrated. However, it is not fully understood how FABP1 expression is dynamically regulated in metabolic disorders. Previous studies have reported that ubiquitin proteasome-mediated degradation of FABP1 is involved, but the mechanism remains unknown.MethodsDysregulated expression of hepatic FABP1 and Derlin-1 was observed in NAFLD patients. We performed mice hepatic tissue co-immunoprecipitation (IP)-based mass spectrum (MS) assays. Derlin-1 interacts with FABP1 and modifies its ubiquitin status, as confirmed by co-IP. The role of Derlin-1 in lipid deposition was tested using adenovirus-mediated overexpression in C57 mice, Derlin-1 overexpression (Derlin-1-OE), or Derlin-1 knockdown (Derlin-1-KO) HepG2 cells.ResultsAs a subunit of the endoplasmic reticulum-associated degradation complex (ERAD), Derlin-1 is negatively associated with NAFLD patients and interacts with and ubiquitinates FABP1. Derlin-1 suppresses FABP1 protein levels and inhibits lipid deposition through a FABP1-dependent pathway. Additionally, Trim25, an E3 ubiquitin ligase present in the endoplasmic reticulum (ER), is recruited to promote Derlin-1-related polyubiquitylation of FABP1, thereby creating a ubiquitin-associated network for FABP1. Overexpression of Derlin-1 ameliorates hepatic steatosis in both C57 mice and HepG2 cells, and contributes to attenuated weight gain, lower liver weight, and visceral fat mass.ConclusionsFABP1, a master enzyme that maintains fatty acid metabolism, undergoes degradation by Derlin-1 through ubiquitin modification. The activation of Derlin-1 in vivo may represent a potential therapeutic strategy for NAFLD.Trial registration:Clinical Trials. gov ID: NCT02118376.
Design and experimental study of a stepped magnetorheological damper with power generation
Authors: Yang Xiaolong , Chen Yingjie , Geng Xiumei , Liu Haiyan
Published: May 2023

Traditional vehicle suspension magnetorheological dampers have problems with low output damping force and require additional energy input to operate, to improve the performance of the vehicle suspension magnetorheological damper, in this paper we propose and investigate a stepped magnetorheological damper structure with power generation, and conducts structural design and magnetic circuit analysis. The effects of different currents, damping gaps, coil slot positions, and coil turns on the damping performance of the stepped magnetorheological damper with power generation are numerically studied. The magnetic circuit sensitivity analysis of the power generation structure and the magnetorheological damper structure is also performed. Experiments have verified the effects of different input excitations on damping and energy-feeding performance, and the results of numerical analysis have been verified. The results show that when the excitation coil is wound for 257 turns, the magnetic circuit requirements are met, and the maximum damping force of the proposed electromagnetic energy fed stepped magnetorheological damper can reach over 4500N, meeting the use requirements.
Keeping Up With The Cardashians: A report on the imbalance between car and Public transportation use during peak hours in Iligan city, Philippines
Authors: Montemor Bernard Louis , Samar Christine Rose , Tantao Nabel
Published: May 2023

During peak hours, traffic in the Philippines may be extremely severe and congested, particularly in big areas such as Metro Manila. This is due to a number of causes, including a large number of automobiles on the road, limited road capacity, and insufficient public transportation networks. It is the same situation in Iligan, despite the city being just a fraction of what Metro Manila is. In this data note, we report a massive imbalance between car and public transport use, especially in peak hours where large volumes of traffic pass through the main arterial road networks, and what this imbalance implies on the current state of the mass transportation system of Iligan city.
The Association between Domestic Violence and Adverse Pregnancy Outcomes in Pregnant Women Seeking Antenatal Care at Public Hospitals in Gedeo Zone: A Prospective Cohort Study
Authors: Ayele Getnet Melaku , Abebe Mesfin , Mulat Seid Shumye , Kebede Selamawit Semagn , Getahun Senait Belay
Published: May 2023

Background: Domestic violence is any harmful behavior by an intimate partner, such as physical, sexual, or psychological abuse. In Ethiopia, domestic violence is a serious and widespread problem. It affects nearly two-thirds (64.6%) of pregnant women and increases the risk of maternal and perinatal morbidity and mortality. Domestic violence during pregnancy is a growing public health problem that may contribute to maternal and perinatal mortality, especially in low and middle income countries. This study aims to assess the association between domestic violence during pregnancy and the risk of adverse pregnancy outcomes in Gedeo zone Public Hospitals, Southern Ethiopia.
Material and Methods: We conducted a prospective cohort study on 142 pregnant women in their third trimester who attended public health institutions in Gedeo Zone for antenatal care. We compared 47 women who were exposed to domestic violence with 95 who were not and followed them until 24 hours after delivery or drop-out. We used SPSS version-24 to analyze data and logistic regression to examine the association between domestic violence and pregnancy outcomes. We reported the results using an adjusted odds ratio with 95% confidence interval and P-value.
Result: Out of 142 women, who completed the follow-up, 47 were exposed to domestic violence and 95 were not. We found a strong link between domestic violence and preterm birth. Women exposed to domestic violence had a four-fold higher risk of preterm birth (AOR= 4.392, 95%CI: 1.117, 6.588) than those who were not. They also had a 2.5-fold higher risk of perinatal death (AOR= 2.562, 95%CI: 1.041, 6.308).
Conclusion: Domestic violence during pregnancy affects many pregnant women in southern Ethiopia and harms their babies. It leads to preterm birth and perinatal death, which can be prevented. The Ethiopian government and other stakeholders need to protect pregnant women from intimate partner violence urgently.
Molecular Docking and Dynamics Simulation revealed the Potential Inhibitory Activity of Piomozide against Kynurenine metabolism targeting the Kynurenine 3-monooxygenase
Authors: Kaladan Kavya Vinil , Sivagurunathan Narmadhaa , Parsanathan Rajesh
Published: May 2023

L-tryptophan metabolism depends on the enzyme kynurenine 3-monooxygenase (KMO). Quinolinic acid, kynurenic acid, and other metabolites having consequences for neurology and psychiatry are mostly derived from L-tryptophan. Furthermore, it was shown that hypertension causes an increase in KMO; hence, inhibiting KMO may aid in preventing both hypertension and cardiovascular diseases. In the current investigation, we used in silico techniques to screen for potential KMO inhibitors. SWISS-MODEL was utilized to simulate the homology with full-length rat KMO complexes with a pyrazoyl benzoic acid inhibitor (PDB:6LKD; sequence identity 80.17%) because the previously available human KMO structure (PDB:5X68) was broken. The flexibility of protein structures was predicted using CABS-flex2.0, and Qmean, Errat, and Procheck were used to improve and validate the 3D models. We used receptor-based screening and drug repurposing. The DrugRep virtual screening server was used for high-throughput docking with FDA-approved, FDA-experimental, and traditional Chinese medicine libraries. The lead-like compounds with good pharmacokinetics characteristics, which may have superior stability and affinity for KMO, were discovered to be the top-scoring drugs from the libraries. Pimozide, an approved pharmaceutical, establishes Van der Waals interactions at ARG85 and TYR99, as well as at TYR398 (4.77Å) and ASN363 (3.16Å) for Pi-Hydrophobic and Halogen Fluorine respectively, all of which are important L-kynurenine binding sites. The co-factor FAD binding nearest residue SER53 formed a hydrogen bond interaction (2.89Å). A molecular dynamic simulation of the FDA-approved drug pimozide exhibited greater interactions and affinities for KMO, which may be able to successfully target the KMO. Pimozide is also used as therapy to treat a variety of psychotic effects, neurodegenerative diseases, and hypertension.
New report of endophytic bacterium Achromobacter xylosoxidans from root tissue of Musa paradisiaca
Authors: Chowhan Papan , Chakraborty Arka Pratim
Published: May 2023

Banana plant (Musa paradisiaca) worldwide cultivated crop harbors many endophytic bacteria. Endophytic bacterium (MRH 11) isolated from root tissue of M. paradisiaca was identified as Achromobacter xylosoxidans (ON955872) which showed positive response in IAA production, phosphate solubilization, catalase production. A. xylosoxidans also showed in vitro antagonism against Curvularia lunata causing leaf spot disease of banana.
Overlapping and Distinct Functions of an Extended Repertoire of KRAS Mutations
Authors: Zimmermann Michael , Mathison Angela , Li Xuan , Leverence Elise , Jensen Davin , Esparza Guadalupe , De Assuncao Thiago Milech , Smith Brian , Volkman Brian , Lomberk Gwen , Urrutia Raul
Published: May 2023

The challenge of interpreting novel genetic variations in genomics is widespread. Previous studies have shown the role of KRAS mutations in heritable and somatic conditions, but more genetic variants have been observed than have been characterized. This study compares a broad genomic landscape of 25 KRAS mutations at three cellular checkpoint levels: ERK and AKT signaling, early cell-intrinsic transcriptional regulation, and secretory inflammatory responses. The results show heterogeneity in KRAS biology, with distinct mutations inducing significantly different levels of phosphorylated ERK, transcriptional profiles, and cytokine protein profiles. This heterogeneity may underlie variation in inter-individual disease patterns and contribute to differences in disease initiation or progression. Transcriptional profiles implicate different extents of feedback from upstream receptor kinases through diverse downstream nuclear targets and immunomodulatory programs. Integrated analysis reveals heterogeneity and potentially actionable outcomes for distinct groups of KRAS mutations in human cells.
First record of mussel larvae occurring in the mantle cavity of littoral-dwelling Mytilus spp. in Svalbard, Arctic
Authors: Olenin Sergej , Minchin Dan , Samuilovienė Aurelija , Kotwicki Lech , Węsławski Jan Marcin
Published: May 2023

After a millennium, the blue mussels Mytilus have returned to the high Arctic region of Svalbard in the beginning of 21st century. Later it was found that not only M. edulis arrived in Svalbard, but a whole complex of sympatric species including M. edulis, M. galloprovincialis, M. trossulus and their hybrids, which may have a wide range of origins from both sides of the North Atlantic. The mussels, which first were found in the sublittoral at the mouth of Isfjorden on the west coast of Spitsbergen island, now are spreading in other areas, and recently were also found in the intertidal zone. Here we report on the finding of viable larvae at the late stages of development (up to pediveliger) that were recovered from the mantle cavity of mussels collected from the lower intertidal shore in late July 2019 and 2021 in Trygghamna, Isfjorden, Spitsbergen. Both the identity of the adult mussels and the larvae was confirmed using by sequencing of barcode region. Larvae ranged in size from 178 to 376 µm, averaging 295 ± 35 µm. They were recovered from mussel specimens ranging from 21 to 57 mm in length, and their ages were estimated, based on the number of rings, from 3 to 12 years. It remains unclear whether the retention of larvae within the mantle cavity results from the intake of near shore concentrations and whether it leads to larviphagy.
Morphotectonic development of surface karst in Western Taurus (Türkiye)
Authors: Şener Mehmet Furkan , Şimşek Mesut , Utlu Mustafa , Öztürk Muhammed Zeynel , Sözbilir Hasan
Published: May 2023

The Taurus Mountains include the most important karstic terrain of Turkey and are divided into three parts the Western, Central, and Eastern Taurus. These mountains include many surface and subsurface karst landforms and the morphometric features of these landforms provide important data on the geomorphological and morphotectonic development of karst areas in the Taurus Mountains. Karstic depressions (poljes and dolines) constitute the most important surface landforms in the Western Taurus Mountains. In this study, a total of 7093 micro depressions (doline) and 74 macro depressions (polje) were detected and morphometric properties were calculated located in the Western Taurus Mountains. The poljes developed within the Beydağları Autochthon and Lycian nappes, while the dolines developed especially in the high plateaus within the Beydağları Autochthon. The morphotectonic development of both landforms is as below shortly. As a result of the northward movement of the African plate from the Early Cretaceous to the Late Miocene, the limestones accumulated in the shallow marine environment were compressed in the north direction and made a counterclockwise movement. Depending on this tectonic activity, the Lycian Nappes from the northwest and the Antalya Nappes from the east thrust over the Baydağları autochthon, and thus nappes, reverse faults and fold systems developed in the study area. The Western Taurus began to continentalization from the Oligocene and began to erode from the Miocene, and also with the erosion, the valley systems began to develop in the Western Taurus. The NE-SW and NW-SE directional normal faults have developed under the influence of extensional tectonics since the Miocene, and this extensional tectonism has caused widespread development of the poljes in the study area. In addition, dolines and paleovalley began to develop in limestone areas at higher elevations with the lowering of the karst base level. All morphometric and morphotectonic processes reveal that the extension of both doline and polje areas in the study area are parallel to the elongation of tectonic structures in the study area.
Analyzing protein concentration from intact wheat caryopsis using hyperspectral reflectance
Authors: Zhang Xiaomei , Hou Xiaoxiang , Su Yiming , Yan XiaoBin , Qiao Xingxing , Yang Wude , Feng Meichen , Kong Huihua , Zhang Zhou , Shafiq Fahad , Han Wenjie , Li Guangxin , Chen Ping , Wang Chao
Published: May 2023

Winter wheat grain samples from 185 sites across southern Shanxi region were processed and analyzed by using a non-destructive approach. For this purpose, spectral data and protein content data of grain and grain powder were obtained. After combining six types of pre-processed spectra and four types of multivariate statistical models, a relationship hyperspectral datasets and grain protein content is presented. It was found that the hyperspectral reflectance of winter wheat grain and powder was positively correlated with the protein contents, which provide the possibility for hyperspectral quantitative assessment. The spectral characteristic bands of protein content in winter wheat extracted based on the SPA algorithm were proved to be around 350–430 nm; 851–1154 nm; 1300–1476 nm; and 1990–2050 nm. In powder samples, SG-BPNN had the best monitoring effect, with the accuracy of Rv2 = 0.814, RMSEv = 0.024, and RPDv = 2.318. While in case of grain samples, the SG-SVM model exhibited the best monitoring effect, with the accuracy of Rv2 = 0.789, RMSEv = 0.026, and RPDv = 2.177. Based on the experimental findings, we propose that a combination of spectral pretreatment and multivariate statistical modeling is helpful for the non-destructive and rapid estimation of protein content in winter wheat.
Association between BMI and the success rate of transaxillary venous port implantation: a retrospective cohort study
Authors: Shao Fei , Zhang Chunling , Jin Yang , Cai Hao , Pang Yunfeng , Wen Gehua , Ma Chong , Wang Guodong , Sun Ziqiang , Jin Song
Published: May 2023

Background
Fully implantable infusion ports are widely used for long-term chemotherapy in cancer patients. Previous studies have only focused on the analysis of complications associated with infusion port implantation, ignoring the causes of unsuccessful infusion port implantation. The purpose of this study was to investigate the association between BMI and the success rate of transaxillary intravenous port implantation in breast cancer patients.
Materials and Methods
To review 361 breast cancer patients who underwent intravenous port implantation from January 2021 to September 2021. Baseline data, surgical data, and complications were collected from the patients, and the success rate of puncture of the axillary vein was recorded. The logistic regression analysis and smoothed curve fitting were used to assess the relationship between body mass index (BMI) and the success rate of axillary venipuncture. In addition, subgroup analyses were performed to explore potential interactions.
Results
Under ultrasound guidance, 67.3% of patients (243/361) had an infusion port implanted by axillary vein puncture. There was a roughly linear relationship between BMI and the success rate of axillary venipuncture. In the multiple regression equation, BMI was significantly and negatively associated with the success rate of axillary venipuncture (OR = 0.83; 95%CI = 0.77–0.89; P＜0.001). Stratified analysis showed that the relationship between BMI and the success rate of axillary venipuncture was stable and unaffected by other variables.
Conclusions
The higher the patient's BMI, the higher the chance of difficult axillary venipuncture or failed cannulation.
A Systems-thinking Approach to Assess the Efficacy of Local Fisheries Management towards Sustainability
Authors: Lukambagire Isaac , Abelson Avigdor , Bhavani Rao R. , N Remya S
Published: May 2023

Unsustainable fishing practices, contribute to a continuous decline in marine fisheries ecosystem resources. However, a lack of understanding on how local systems can be used in fisheries management is evident in literature. This study used a systems-thinking approach to show how local fisheries management practices could be used to promote sustainability in Alappad, Kerala. Systems-thinking entailed understanding of the complex interdependent relationships between the economic, environmental and social factors of a fishery system. The first step involved conducting a systematic literature review and data extraction from peer review journals and official websites. These were analyzed using Excel and R. The second step involved the use of system thinking models comprising causal system dynamics and systems actor mapping to present complex information as interactive relationship maps. Findings highlighted the significance of collaborative decision-making procedures and the necessity of strong governance frameworks for efficient fisheries management. Thus, there’s a need to adopt co-management strategies that take into account practical and proactive knowledge of the fishery operations through effective research methods and local involvement in decision-making processes. This study contributes to the continuing discussion about sustainable fisheries management practices and offers policymakers, managers, and researchers a useful foundation for comprehending the intricate dynamics of the fisheries system in Alappad panchayat and creating effective management measures. These results would contribute to the sustainability of coastal communities and the fisheries on which they depend in Kerala and other regions throughout India and the world.
Hypertension Recommended Self-care Knowledge, Attitude, Practice and Its Predictors among Hypertensive Patients in Ethiopia: A Cross Sectional Study
Authors: Wake Addisu Dabi
Published: May 2023

Introduction:
Globally, hypertension is a significant public health challenge. Elevated BP is responsible for 7.6 million deaths per annum globally, which is greater than any other risk factors. The aim of the study was to assess the hypertension recommended self-care knowledge, attitude, practice and its predictors among hypertensive patients on follow-up clinic.
Methods
A cross-sectional survey was done on 120 hypertensive patients who were on follow-up clinic at Asella Referral and Teaching Hospital. The data were collected from patients from 23/12/2020 to 16/01/2021 by face-to-face interview and medical records review. Data were entered into Epi Data version 4.2.0.0 and exported to SPSS version 23.0 for statistical analysis.
Results
The level of good knowledge, favorable attitude, and good practice towards recommended Self-care was 60.0%, 52.5%, and 50.8%, respectively. The multivariable logistic regression analysis showed that; formal education [AOR = 3.615, 95%CI (1.262, 10.352), p = 0.017], and receiving information about Self-care from healthcare providers during follow-up [AOR = 3.307, 95%CI (1.226, 8.926), p = 0.018] were factors significantly associated with knowledge towards recommended Self-care. Having good knowledge towards Self-care [AOR = 13.449, 95%CI (4.794, 37.728), p = 0.000] was significantly associated with attitude towards recommended Self-care. Receiving information about Self-care from healthcare providers during follow-up was 3.887 times [AOR = 3.887, 95%CI (1.232,12.261), p = 0.021], having good Knowledge towards Self-care [AOR = 3.195, 95%CI (1.161,8.794), p = 0.025], having a favorable attitude towards Self-care [AOR = 3.093, 95%CI (1.198,7.990), p = 0.020] were factors significantly associated with practice towards recommended Self-care.
Conclusion
The level of good knowledge, favorable attitude, and good practice towards recommended Self-care among hypertensive patients on follow-up was unsatisfactory. Health education programs about hypertension self-care are needed to take place to improve their knowledge, attitude, and practice of the patients.
BRCA testing and Japanese original testing criteria for hereditary breast and ovarian cancer covered by public health insurance
Authors: Ohta Kouji , Itoh Tomoko , Katoh Jun
Published: May 2023

In Japan, BRCA1/2 testing has been made available to the public as a companion diagnostic test for olaparib since July 2018. This testing indication has been expanded to diagnosis of hereditary breast and ovarian cancer. The Japanese breast cancer society (JBCS) issued original guidance and criteria to estimate eligibility for BRCA testing among breast cancer patients. This retrospective study aimed to evaluate the current Japanese situation of BRCA testing and the utility of JBCS criteria. We retrospectively reviewed medical records of 1,136 early breast cancer patients. The number of the patients who were eligible for BRCA testing based on JBCS criteria was retrospectively counted. The relationship between the six categories of JBCS and BRCA pathogenicity was evaluated using multivariate analysis. Among the entire cohort, 40.2% met at least one criterion. Being diagnosed with breast cancer at 45 years or younger of age, having two or more primary breast cancers, and being diagnosed with triple negative breast cancer at 60 years or younger significantly increased the likelihood of having BRCA mutation (OR: 8.8, 20.8, 8.6, respectively). In addition, the patients that met two or more testing criteria had higher rates of BRCA mutation than those that met only one criterion. This study suggests that approximately 40% of early breast cancer patients are potential candidates for BRCA testing and/or genetic counseling based on JBSC criteria. Prioritizing the six categories may make the criteria more applicable for use in the current Japanese clinical setting, as genetic practice has limited specialist human resources.
Moxa Cone Moxibustion at Zusanli(ST36) acupoint alleviate myelosuppression mouse induce by CTX and promote hematopoietic cell proliferation via HPA axis
Authors: Tan Li , Li Zhilin , Cao Nan , Li Jing , Ye Zhiyu , Chen Hongda , Wang Ning
Published: May 2023

Objective. Clinical studies have reported that moxibustion alleviates the side effects of chemotherapy, such as myelosuppression; however, its therapeutic mechanisms remain unclear. We investigated whether moxa cone moxibustion (MCM) promotes recovery from chemotherapy-induced myelosuppression via the hypothalamus-pituitary adrenal (HPA) axis.
Methods. A myelosuppression model was established in mice using cyclophosphamide (CTX), and they were grouped into control, model, and moxibustion groups. In the moxibustion group, the mice received MCM at ST36 for 7 days. The peripheral blood cells were detected using an automatic blood cell analyzer; serum levels of corticotropin-releasing hormone (CRH), corticotropin (ACTH), corticosterone (CORT) and glucocorticoid receptor (GRF) a/β were detected using enzyme-linked immunosorbent assay (ELISA); the expression and cell cycle of bone marrow hematopoietic cells were detected using flow cytometry (FC); and BGISEQ Instrument model-DNBseqTM Platform was used for RNA sequencing. The differentially expressed genes (DEGs) were subjected to gene ontology (GO) function enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
Results. Compared with the control group, the contents of the peripheral blood and LSK and HSC in the moxibustion group were significantly higher (P<0.05). Following moxibustion therapy, the S and G2 phases of bone marrow hematopoietic stem cells decreased significantly, while the G1 phase increased. The CRH, ACTRH, and CORT levels decreased (P<0.05), while GRF-a and GRFβ levels increased (P<0.05). Additionally, 193 DEGs were down-regulated in the control group and up-regulated in the moxibustion group, while 481 DEGs were up-regulated in the control group and down-regulated in the moxibustion group. GO analysis revealed that cross-DEGs were enriched in cell-cell junction, membrane raft, membrane microdomain, and T-cell receptor complex involved in T-cell activation, T-cell differentiation, and lymphocyte differentiation, which exerted the MF of cytokine binding, receptor activity, and activity. KEGG analysis revealed that hematopoietic cell lineage, primary immunodeficiency, cytokine-cytokine receptor interactions, and other pathways were significantly enriched in the moxibustion group.
Conclusion. MCM could alleviate myelosuppression induced by CTX and promote hematopoietic cell proliferation via the HPA axis and promote the proliferation and differentiation of bone marrow hematopoietic cells by regulating gene expression.
USP22 controls tumorigenicity by regulating interferon responses and the stability of the tumor suppressor PML
Authors:   , Kowald Lisa
Published: May 2023

Post-translational modifications (PTMs) of cell fate regulating proteins determine their stability, localization and function and control the activation of cell protective signaling pathways. Particularly in aberrantly dividing cancer cells the surveillance of cell cycle progression is essential to control tumorigenicity. In a variety of carcinomas, lymphomas and leukemias, the tumor-suppressive functions of the apoptosis- and senescence-regulating promyelocytic leukemia protein (PML) is controlled by numerous PTMs. PML poly-ubiquitylation and polySUMOylation at several lysine (K) residues induce PML degradation that is correlated to a progressive and invasive cancer phenotype. Besides several known E3 ubiquitin protein ligases that are involved in PML degradation, less is known about PML-specific deubiquitylases (DUBs), the respective DUB-controlled ubiquitin conjugation sites and the functional consequences of PML (de)ubiquitylation. Here, we show that the pro-tumorigenic DUB USP22 critically regulates PML protein stability by modifying PML residue K394 in advanced colon carcinoma cells in vitro and that this modification also impacts the homeostasis and function of the leukemia-associated mutant variant PML-RARα. We found that ablation of USP22 decreases PML mono-ubiquitylation and correlates with a prolonged protein half-live in colon carcinoma and acute promyelocytic leukemia (APL) cell lines. Additionally, silencing of USP22 enhances interferon and interferon-stimulated gene (ISG) expression in APL cells in vitro, which together with prolonged PML-RARα stability increases the APL cell sensitivity towards differentiation treatment. In accordance with the novel roles of USP22 as suppressor of the interferon response in human intestinal epithelial cells (hIECs), our findings imply USP22-dependent surveillance of PML-RARα stability and interferon signaling in human leukemia cells, revealing USP22 as central regulator of leukemia pathogenesis.
Sequential flows by irrelevant operators
Authors: Ferko Christian , Sethi Savdeep
Published: May 2023

We explore whether one can T\overline{T}TT¯ deform a collection of theories that are already T\overline{T}TT¯-deformed. This allows us to define classes of irrelevant deformations that know about subsystems. In some basic cases, we explore the spectrum that results from this procedure and we provide numerical evidence in favor of modular invariance. We also study the flow of the classical Lagrangian for free bosons and free fermions under successive deformations. Some of the models found by sequentially flowing are likely to have interesting holographic interpretations.