bluuebunny/crossref_metadata_2025 huggingface dataset:
201 Development of fertility programs to achieve high 21-day pregnancy rates in high-producing dairy cows
Authors: Fricke Paul , Carvalho Paulo , Wiltbank Milo
Published: July 2019

The initial impact of the original Ovsynch protocol on 21-d pregnancy rates in U.S. dairy herds was to increase AI service rates; however, a deeper understanding of the physiology underlying the Ovsynch protocol has allowed for modifications to the original protocol that dramatically increase in pregnancies per artificial insemination (P/AI). A key factor affecting P/AI to timed AI is the response to each hormonal treatment of the Ovsynch protocol. Cows that ovulate to the first GnRH treatment of the Ovsynch protocol (G1) have more P/AI than cows that fail to ovulate. The association between progesterone (P4) concentrations at each treatment during the Ovsynch protocol and P/AI was analyzed based on data from 7,792 cows across 14 experiments. Cows with P4 concentrations from 0.5 to 6.0 ng/mL at G1, >1.0 ng/mL at PGF2α, and <0.4 ng/mL at the last GnRH treatment had the most P/AI. Presynchronization strategies that combine GnRH and PGF2α induce ovulation in anovular cows and optimize P4 concentrations at G1 and the PGF2α treatments of the Ovsynch protocol to increase P/AI. A subgroup of cows, however, fail to undergo complete luteal regression after the PGF2α treatment of the Ovsynch protocol, particularly multiparous cows that initiate G1 in a low P4 environment and cows with a CL that has not yet acquired luteolytic capacity. Addition of a second PGF2α treatment 24 h after the first in the Ovsynch protocol increases the percentage of cows with complete luteal regression thereby increasing P/AI. The key to increasing P/AI to Resynch protocols is to segregate nonpregnant cows based on the presence or absence of a CL and to treat cows without a CL with exogenous P4 during the Resynch protocol. Adoption of fertility programs developed using these concepts have yielded annualized 21-d pregnancy rates that exceed 30% in healthy, high-producing dairy herds.
211 Young Scholar Presentation: Developing and validating a heat stress model in dairy cows
Authors: Al-Qaisi Mohmmad , Horst Erin A , Kvidera Sara , Mayorga Edith J , Abeyta Megan A , Goetz Brady M , Timms Leo , Baumgard Lance H
Published: July 2019

Heat stress (HS) negatively impacts production parameters, including milk yield and composition, growth and reproduction. Precisely studying HS typically requires expensive climate-controlled facilities, resources often inaccessible to most scientists. Therefore, we evaluated the efficacy of using an electric heat blanket (EHB) as an alternative and cost-effective method to study HS and determine whether EHB-induced hyperthermia affects physiological and production parameters similar to natural HS. This was the first proof of concept study examining this model. Results indicated increased body temperature indices (i.e., rectal temperature and respiration rate) and reduced DMI and milk yield, as well as altered milk composition similar to natural and climate-controlled HS studies. In experiment 2, we examined the EHB model in combination with a pair-feeding (PF) design. By employing the PF design, we clearly illustrated that a lowered nutritional plane explains only approximately 50% of the decreased milk yield in HS cows, with the remaining portion due to changes in post-absorptive changes in nutritional physiology. In addition, the EHB blunted adipose tissue mobilization and increased plasma biomarkers of muscle catabolism. Results from this study validated that the EHB is a valid model to study HS; therefore, experiment 3 was designed to assess nutritional HS mitigation strategies using the EHB model. Dietary electrolyte, osmolyte, and energetic compounds (EOEC) were evaluated as re-hydration therapy on body temperature indices in HS lactating Holstein cows. Skin temperature was increased in EOEC-supplemented cows relative to controls which suggests that EOEC increased heat dissipation. In addition, EOEC-supplemented cows had increased glucose and insulin levels compared with controls which is due to the energetic compounds (i.e., dextrose) present in the EOEC supplement. Collectively, employing EHB model provides an excellent new platform for discovery research and evaluating pragmatic HS mitigation strategies.
227 Evaluation of reimplant timing with Revalor-200 on steer performance and carcass characteristics
Authors: Coulson Caitlin A , Boyd Bradley M , Hilscher Henry , Nuttelman Brandon L , Crawford Grant I , MacDonald James C , Erickson Galen E
Published: July 2019

The objective of this study was to evaluate the optimal time to reimplant following an initial implant on performance and carcass characteristics when steers were fed for 180 d. Crossbred steers (n = 800; initial BW = 330 ± 25 kg) were utilized in a generalized randomized block design and assigned randomly to pen, and pen assigned randomly to one of five treatments. Treatments included 5 different days on terminal (DOT) implant (160, 120, 100, 80 or 40 DOT). All steers were given an initial implant (80 mg trenbolone acetate (TBA) + 16 mg estradiol; Revalor-IS) on d 1. Terminal implant consisted of 200 mg TBA + 20 mg estradiol (Revalor-200). Dry matter intake was the least for 40 DOT (P ≤ 0.04), with no differences between the other treatments (P ≥ 0.11). Carcass-adjusted final BW responded quadratically (P = 0.03) with 100 DOT having the greatest final BW but was not different from 120 DOT (P = 0.82). Carcass-adjusted ADG and G:F responded quadratically (P = 0.02 and P < 0.01, respectively). When solved for the first derivative, carcass-adjusted ADG and G:F were maximized at 99 and 87 DOT, respectively. However, there was less than a 2% difference in carcass-adjusted ADG and G:F between 80 and 120 DOT. Hot carcass weight responded quadratically (P = 0.03), with the greatest HCW at 100 DOT. Solving for the first derivative, HCW was optimized at 104 DOT. There were no differences in back fat, marbling, or yield grade (P ≥ 0.27). Overall, administering an initial implant followed by a terminal implant 85 to 105 d prior to harvest increased gain, efficiency, and HCW in steers when fed for 180 d. Minimal changes in performance and carcass characteristics when steers are reimplanted 80 to 120 d prior to harvest suggests flexibility in reimplanting windows.
334 Evaluation of sources of RUP fed to Holstein steers in a simulated organic beef production system
Authors: Schumacher Elizabeth , Erickson Galen E , MacDonald James C , Klopfenstein Terry , Carlson Zachary E , Hilscher Henry , McPhillips Levi , Wilson Hannah C
Published: July 2019

This study evaluated rumen undegradable protein (RUP) sources on performance of lightweight Holstein steers fed diets that simulated the requirements for organic beef production. Holstein steers (n = 59; initial BW = 213kg) were fed individually for 214 d. Diets contained 30% alfalfa haylage, 5% supplement to meet mineral requirements, and differing proportions of dry rolled corn and a protein source to supply RUP. Steers were assigned randomly to dietary treatments: a protein-deficient control (CON), field peas (FP), field peas and fish meal (FPFM), soybean meal (SBM), and a soybean meal treated to increase RUP (Soypass; SP). Diets were reformulated by phase as MP requirements decreased. Experimental design was completely randomized with experimental unit being steer. A tendency (P = 0.09) for a difference in ADG in the first phase was observed, with steers fed any RUP source having greater ADG than CON. No difference (P ≥ 0.22) was detected for ADG in subsequent phases as RUP sources were displaced with corn. Intakes were not different (P ≥ 0.26) among treatments during the first and last phase, but during phase two and three calves fed SP had greater DMI (P ≤ 0.03) compared with FP or FPFM, with the CON and SBM groups being intermediate. In the first phase, calves in the CON group had significantly (P < 0.01) lower G:F compared to treatments with RUP, which were not different from one another. No differences (P ≥ 0.31) were observed between treatments for G:F during the final three phases. These data suggest that supplementing RUP improved ADG and G:F, but source of RUP did not impact performance of lightweight Holstein steers when inclusions were varied to account for differences in RUP content of each protein source. These animals compensated for a deficiency in MP early in the feeding period.
343 Feedlot performance and carcass characteristics of steers fed diets containing steam-flaked grain and corn silage from Enogen® Feed Corn
Authors: Baker Adrian , Veloso Vanessa de Aguiar , Baker Adrian , Barros Lucas
Published: July 2019

Enogen Feed Corn (EFC; Syngenta Seeds, LLC) is genetically modified to express high concentrations of amylase. Our objective was to evaluate EFC as corn silage and as steam-flaked corn in diets fed to finishing cattle. A 2 x 2 factorial experiment was conducted with steers (n = 960; 388 ± 7.4 kg initial body weight), with factors consisting of silage source (Control or EFC) and grain source (Control or Enogen). Steers were blocked by initial body weight, assigned randomly within block to treatments, housed in 48 pens with 15 or 25 cattle per pen, and harvested after 138, 152, or 166 days on feed. Grains were steam flaked to densities of 360 or 386 g/L for Control and EFC, respectively. Diets (dry basis) consisted of 8% corn silage, 2% alfalfa hay, 74.5% flaked corn, 12% Sweet Bran, and supplement. Incidences of liver abscesses and carcass weights were recorded at harvest, and longissimus muscle area, 12th-rib subcutaneous fat thickness, marbling score, and USDA yield and quality grades were determined after 36 h of refrigeration. There were no interactions between grain source and silage source (P > 0.05) for feedlot performance. Cattle fed diets containing EFC silage consumed less dry matter (P < 0.01) and efficiency of gain was improved by approximately 5.3% (P < 0.01) compared to cattle fed Control silage. Average daily gain and dry matter intake were unaffected by grain source, but cattle fed EFC grain were less efficient (P = 0.02) compared to cattle fed control grain. Carcass weight was greater for cattle fed the combination of EFC silage and Control grain compared to other treatments (P < 0.05), but liver abscess incidence and other carcass measurements were unaffected by grain or silage source. Feeding Enogen Feed Corn as corn silage, but not as grain, improved feedlot performance of steers.

Keywords: Enogen, corn silage, amylase
340 Impact of essential oils blend on beef cattle performance and carcass characteristics in diets with increasing corn silage inclusions
Authors: Wilson Hannah C , Hilscher Henry , Carlson Zachary E , Boyd Bradley M , Watson Andrea K , MacDonald James C , Erickson Galen E
Published: July 2019

A feedlot study was conducted comparing a natural feed additive (essential oils blend) at varying silage inclusions on receiving and finishing performance. The study utilized 480 crossbred steers (initial shrunk BW 296 kg ± 7.72 kg) in 48 pens with 10 steers/pen and 8 pens per treatment. Treatments were designed as a 2 × 3 factorial with 3 inclusions of corn silage [14, 47, 80 %; dry matter (DM) basis] with or without (+, -) the inclusion of an essential oils (EO) blend. All treatment diets contained 16% modified distillers grains plus solubles and 4% supplement with dry-rolled corn replacing corn silage (CS) on a DM basis. Steers were fed at 80% CS inclusion and adapted to 47% and 14% CS over a 10 and 24-d period, respectively. Cattle fed 80% CS were fed for 238 days, 47% CS for 195 days, and 14% CS were fed for 168 days to a common backfat of 1.28 cm (P > 0.59). There were no interactions for corn silage inclusion and the inclusion of essential oils on final body weight (FBW), dry matter intake (DMI), average daily gain (ADG), gain to feed ratio (G:F), hot carcass weight (HCW), longissimus muscle (LM) area, marbling, or calculated yield grade (CYG; P > 0.15). There was no significant difference for FBW, DMI, ADG, G:F, HCW, LM area, marbling, or CYG for cattle fed EO or no EO. However, there was a quadratic response for FBW, ADG, G:F, HCW, marbling and CYG with increased inclusion of corn silage (P < 0.04). Feeding corn silage at greater inclusions decreased animal performance but increased final body weight when fed to an equal fatness. Essential oils did not affect animal performance or carcass characteristics. However, feeding greater amounts of corn silage can be an economical way to finish cattle.
349 The use of low antioxidant mineral concentrations in the growing diet as a method to increase marbling deposition in feedlot cattle
Authors: Jaborek Jerad R , Relling Alejandro E
Published: July 2019

The presence of reactive oxygen species has been associated with the differentiation of pre-adipocytes into lipid filled mature adipocytes in vitro. We hypothesized offering a diet without supplemental antioxidant minerals (Cu, Mg, Zn, and Se) to steers during the growing phase, a time when intramuscular fat cells are believed to be proliferating, would promote differentiation of pre-adipocytes, leading to more desirable marbling scores compared with steers supplemented to meet their mineral requirements. After adaptation to the feedlot, 168 Sim-Angus steers were divided into four weight blocks, placed in one of twenty-four pens, and randomly assigned a dietary treatment. Dietary treatments were: 1) no supplemental (Cu, Mg, Zn, and Se) minerals; 2) control diet with supplemented minerals to meet the mineral requirements of growing beef steers (NRC, 2016); 3) Cu, Mg, Zn, and Se concentrations supplemented at twice the concentration of the control diet. After dietary treatments were applied for the 4-wk long growing phase, steers were offered a common finishing diet until reaching a similar backfat thickness until harvest. Feedlot performance and carcass data were analyzed in SAS with pen as the experimental unit in the following model: Yij = μ+Di+wj+eij, where Di was the fixed effect of diet, wj was the random effect of weight block, and eij was the random error. No significant (P > 0.35) treatment effects were found for feedlot performance and carcass measurements. The feedstuffs in the basal diet may have contained sufficient concentrations of antioxidant minerals to meet the mineral requirements of the steers and mask differences in marbling. Additionally, offering a similar diet during the finishing period may have resulted in compensatory marbling growth, which offset marbling differences after the growing phase and resulted in uniform marbling accumulation across dietary treatments.
357 Evaluation of growth performance and physiology of nursery pigs fed deoxynivalenol (DON) contaminated feed supplemented with commercial feed additives (CFA)
Authors: Rho Youngji , Voth Conor , Buis Quincy , Trott David , Huber Lee-Anne , Kiarie Elijah
Published: July 2019

Four commercial feed additives (CFA) were evaluated for efficacy in detoxifying deoxynivalenol (DON) in diets fed to 144 nursery pigs (BW 9.80+/- 0.5kg; 2 gilts, and 2 barrows/pen). Six test corn-soybean-meal based diets were: 1) positive control (PC), formulated with clean corn (NC), 2) formulated with contaminated corn (5.5 ppm DON), 3) NC with enzyme+binder1 (NCB1), 4) NC with clay (NCC), 5) NC with enzyme+binder2 (NCB2) and 6) NC with sodium metabisulfite (NCP). Diets were allocated to pens (n = 6) based on BW and fed ad-libitum for 4-wk; BW and feed intake were monitored weekly. At the end of wk-1, one pig/pen was euthanized for tissue collection. Assayed DON concentration for PC, NC, NCB1, NCC, NCB2 and NCP were 0.38, 2.3, 2.3, 2.3, 2.4, and 1.9 ppm, respectively. Diet effects were only observed for ADG and G:F in wk-1; pigs fed NCP had higher (P = 0.01) ADG compared to pigs fed NC and NCC, whilst pigs fed other diets had intermediate and similar ADG (P > 0.05) to pigs fed NC or NCC. Pigs fed NCP showed higher (P = 0.02) G:F relative to pigs fed other diets. The final BW was 31.3, 30.8, 30.4, 30.8, 31.1 and 32.2kg for PC, NC, NCB1, NCC, NCB2 and NCP-fed pigs, respectively. There were no (P > 0.05) diet effects on ADFI throughout the study. There was no (P > 0.05) diet effects on liver and spleen weight, jejunum morphology and concentration of plasma creatinine and urea. Ceca digesta concentration of citric acid was higher (P = 0.03) in pigs fed NCP than pigs fed NCB2. In conclusion, the lack of differences between PC and NC suggested pigs were tolerant to higher DON levels than the regulated dietary maximum of 1 ppm. Improved ADG and G:F due to NCP vs. NC was transient and was not linked to physiological responses.
356 Young Scholar Presentation: An applied approach to studying heat stress: Effects of cyclic heat and zinc supplementation on body temperature, gut health, and pork quality
Authors: Feldpausch Julie A , Mills Kayla M , Duttlinger Alan W , Elly S M , Kim Yuan H B , Radcliffe John S , Rambo Zach , Richert Brian T
Published: July 2019

Heat stress (HS) has depletory effects on intestinal morphology, induces metabolic changes, and disposes pigs to oxidative stress. Zinc has roles in gut health, nutrient and insulin mediated metabolism. To better understand the effects of cyclic heat and dietary Zn supplementation on gut integrity, carcass composition, and pork quality, commercial crossbred mixed-sex pigs (n = 400; initially 72.2 kg) were housed under either thermoneutral (TN; 18.9–16.7°C) or cycling HS conditions simulating chronic summer heat (30°C/26.7°C for 12h:12h on days 24–65) with acute heat periods of 32-33°C/29-30°C for 12h:12h on days 21–24, 42–45, and 63–65. Treatments were arranged in a 2×2×2 factorial with main effects of environment (HS vs. TN), added Zn level (50 vs. 130 mg/kg available Zn), and added Zn source (inorganic from ZnO vs. organic from Availa®Zn; Zinpro Corp, Eden Prairie, MN). Relative to TN, HS elevated (P < 0.050) body temperatures during the growing period. Heat stress was correlated with ileal villus height (r=-0.51, P = 0.015) and HSP-70 expression (r=0.46, P = 0.041). Growth was reduced such that carcasses from HS pigs were lighter (P = 0.011) and exhibited improved carcass quality with higher (P = 0.001) 24-hour loin pH, decreased (P = 0.034) drip loss, and greater (P < 0.050) subjective color and firmness scores compared to TN carcasses. Relative to TN, loin chops and sausage patties manufactured from the HS carcasses had similar oxidative stability (CIE L*a*b* color, 2-thiobarbituric acid reactive substances) throughout a 10-day simulated retail display. The HS induced fatty acid profile differences in the pork product but were not sufficient to shift IV (P > 0.10). Belly firmness and slice lean were also unaffected (P > 0.10) by HS. Zinc supplementation had minimal impact on the carcass quality characteristics studied. Further research is necessary to better understand the impact of HS duration, combination with additional stressors, and refine the ability to utilize thermal monitoring to manage negative impacts of HS.
351 Neonatal birth weight effects on gilt development growth and first parity reproductive efficiency
Authors: Supakorn China , Lents Clay A , Martinez Xochitl , Vallet Jeff , Dean Boyd R , DeDecker Ashley , Stalder Kenneth J
Published: July 2019

There has been a great deal of interest in gilt development characteristics that predict gilt growth and reproductive traits and which could be measured and manipulated early in the gilt’s lifetime. The objective of the study was to determine neonatal birth weight effects on gilt development growth performance and parity 1 sow reproductive performance traits. Data were collected from 1,052 litters housed at Circle 4 Farms, Milford, UT. A total of 2,960 crossbred Large White x Landrace maternal line gilts entered the research gilt development unit. Gilts were categorized by their individual neonatal birth weight into 3 groups Group I (≤ 1.1 kg; n = 772), Group II (1.2 to 1.5 kg; n = 1,356), and Group III (≥ 1.6 kg; n = 832). Growth and reproductive trait least square means (±SE) for each birth weight group were analyzed and compared among birth weight groups using PROC GLM. Fixed effects in the model included birth weight, farm, and development diet with the random effect of pen within a room. Neonatal birth weight group was a significant (P < 0.05) source of variation for gilt growth in development, number born alive, and litter birth weight at first parity. Gilts from the largest birth weight group had significantly (P < 0.05) larger BW at 100 (45.1 ± 0.3 kg), and BW 200 days (125.7 ± 0.7 kg), faster average daily gain (0.81 ± 0.005 kg), larger BW at puberty (137.7 ± 0.8 kg), larger BW at farrowing (201.1 ± 1.2 kg), larger BW at post-weaning (195.0 ± 1.0 kg), larger number born alive (11.8 ± 0.1), larger litter birth weights (18.2 ± 0.2 kg). The largest birth weight group tended (P > 0.05) to wean more pigs (9.0 ± 0.2) and have greater litter weaning weights (48.8 ± 1.04 kg) at first parity when compared to gilts from the other two birth weight groups. Improving neonatal birth weight will improve gilt development and productivity through first parity.
361 Increasing duration of feeding high dietary lysine and energy before farrowing on colostrum quality and yield in mixed parity sows
Authors: Swanson Analicia J , Gourley Kiah M , Jones Cassandra K , DeRouchey Joel M , Tokach Mike D , Dritz Steve S , Goodband Robert D , Frederick Brent
Published: July 2019

A total of 472 mixed parity sows were used from d 106 of gestation until weaning to determine the effects of Lys and energy intake in the last 7 d before farrowing on colostrum production, composition, and immunoglobulin G concentrations. On d 106 of gestation, sows were weighed, blocked by parity and weight, and allotted to dietary treatments. Treatments were based on corn-soybean meal diets fed to provide 1) 12.5 g SID Lys and 6.5 Mcal/d ME from d 107 to 112 of gestation, then 28 g SID Lys and 9.4 Mcal/d ME from until farrowing; 2) 12.5 g SID Lys and 6.5 Mcal/d ME from d 107 to 112 of gestation, then 40 g SID Lys and 13.3 Mcal/d ME from until farrowing; 3) 40 g SID Lys and 13.3 Mcal/d ME from d 107 of gestation until farrowing. After birth of the first piglet, a 50 mL colostrum sample was collected. Colostrum samples were analyzed for fat, protein, total solids, lactose, and IgG concentrations. Individual piglets were weighed at birth and 24 h to determine colostrum intake and yield. Data was analyzed for treatment within parity and treatment main effects. There was a treatment within parity difference observed where sows on treatment 3 had decreased (P < 0.05) fat and total solids concentrations compared to treatment 1 sows, with no evidence for difference within gilts. IgG concentrations tended to increase (P = 0.081) in females fed treatment 2 compared to treatment 1, regardless of parity. Total protein and lactose concentrations as well as colostrum intake and yield were similar across treatments, regardless of parity. In conclusion, feeding high Lys and energy for 7 d before farrowing decreased fat and solid concentrations in colostrum in sows, but did not impact other colostrum nutrients or colostrum production.
PSV-3 Effects of space allowance and marketing strategy on growth performance of pigs raised to heavy market weights
Authors: Lerner Annie B , Tokach Mike D , DeRouchey Joel M , Dritz Steve S , Goodband Robert D , O’Quinn Travis G , Gonzalez John M , Rice Emily A , Allerson Matt W , Price Hannah E , Boler Dustin D , Dilger Anna C , Shackelford Steven D , Fields Brandon , Woodworth Jason C
Published: July 2019

A total of 976 pigs (PIC 327×L42, initially 22 ± 1.5 kg BW) were used in a 160-d study to determine the influence of space allowance and marketing strategy on performance of pigs raised to heavy market weights (165 kg). Pens were blocked by location and allotted to 1 of 6 treatments with 8 pens/treatment. The first four treatments reduced space allowance/pig via initial pen stocking density: 14 pigs/pen (1.20 m2/pig), 17 pigs/pen (0.98 m2/pig), 20 pigs/pen (0.84 m2/pig), or 23 pigs/pen (0.73 m2/pig). The fifth treatment began with 25 pigs/pen (0.67 m2/pig) and the heaviest 3 pigs/pen were removed on d 93, then on d 122 pens were marketed to a common inventory of 20 pigs/pen, and on d 147 marketed to a common pen inventory of 17 pigs/pen. The sixth treatment began with 23 pigs/pen (0.73 m2/pig) and were marketed to a common inventory of 20 pigs/pen on d 108 and marketed to a common inventory of 17 pigs/pen on d 147. Data were analyzed using PROC GLIMMIX with pen as the experimental unit. Overall (d 0 to 160) ADG, ADFI, and final BW decreased (linear, P < 0.001) and G:F increased (quadratic, P = 0.042) as space allowance decreased. When comparing treatments with multiple marketing events (treatments 5 and 6) to treatment 4, there was no evidence for differences (P > 0.05) for overall ADG or ADFI; however, overall G:F was improved (P < 0.05) for pigs initially stocked at 0.67 m2/pig and marketed four times compared to both treatments that initially allowed 0.73 m2/pig, regardless of marketing structure. These results indicate that decreasing space allowance of heavy weight pigs reduces growth, feed intake and final BW, although use of multiple marketing events prior to final marketing may allow for increased number of pigs marketed/pen while balancing reduced growth performance often associated with increased stocking density. http://www.conferenceharvester.com/
PSV-5 Effects of two products containing tiamulin hydrogen fumarate on drinking water intake of weaned pigs
Authors: Vonnahme Kimberly A , Amodie Deb , Short Thomas , Mellencamp Marnie , Mueller Adam , Galina Lucina
Published: July 2019

Tiamulin hydrogen fumarate is commonly used in water to treat enteric or respiratory disease caused by susceptible pathogens in nursery pigs. This study compared nursery-age pig consumption of: 1) non-medicated water; 2) water containing Triamulox™ (Zoetis, Parsippany, NJ) at 4.763 ppm; and 3) water containing Denagard® (Elanco, Greenfield, IN) at 4.763 ppm (10.5 mg/lb; per label directions for respiratory pathogens). The hypothesis was that water intake would not be different among treatments. The 5-day test period was repeated four times, with a 5-day washout period between replicates. Three hundred pigs were individually weighed for randomization to pen (n = 30 pens) in 2 barns with 15 pens/barn (5 pens/barn/treatment). Water was provided via a 5-gallon reservoir cup water system in each pen. Every 24 hours, residual water weights were taken from each reservoir by draining the water and weighing the water using a platform scale. The weight of daily water additions was also recorded. Pig body and feed weights were taken at the beginning and end of each replicate. Three replicates occurred during the first 30 days after entry to the nursery. After the 3rd replicate, the number of pigs per pen was reduced from 10 to 6 to provide adequate pen space. Ten days prior to pigs leaving the nursery (day 70 of age), a 4th replicate was performed. There were no treatment effects on ADG (P ≥ 0.23) or on pig body weights at the beginning or end of any replicate (P ≥ 0.51). Water intake and gain/feed were not different among treatments (P ≥ 0.16 and P ≥ 0.44, respectively). Water consumption of nursery-age pigs offered non-medicated water or water containing either Triamulox™ or Denagard® was similar in the post-weaning period (days 21–51) and immediately preceding exit from the nursery (day 76–80).
PSI-4 Comparison of analgesics for control of lameness-associated pain in lactating dairy cattle
Authors: Warner Rochelle , Kleinhenz Michael , Ydstie Joshua , Schleining Jennifer , Wulf Larry , Coetzee Johann , Gorden Patrick
Published: July 2019

Both the economic loss and welfare implications of lameness vastly impact the dairy industry. There is a need for effective modalities of analgesia to minimize welfare concerns regarding lameness associated pain in lactating dairy cattle. To date, there are no labeled products with pain control indications available for lactating dairy cattle in the United States. Non-steroidal anti-inflammatory drugs (NSAIDs), like meloxicam and flunixin meglumine, are commonly used by veterinarians for pain management. Data is limited as to their efficacy in lameness-associated pain management. The overarching goal of this study was to compare the analgesic effects of flunixin meglumine (IV) and meloxicam (PO) in lactating dairy cattle with experimentally induced lameness via intra-articular injection of amphotericin B. We hypothesized that lameness would improve under meloxicam treatment compared to flunixin meglumine or no treatment control. A double blinded, randomized controlled trial involving positive and negative controls created the framework to assess efficacy parameters. Parameters included pressure algometry, pressure mat analysis, lameness score, infrared thermography imaging, substance P, and cortisol concentrations of 48 lactating Holstein cows across a six-day time period. Intra-articular injection with amphotericin B resulted in mild transient lameness. Results indicated statistically significant treatment by time effects for several parameters. Visual lameness scores showed flunixin meglumine was equally effective to meloxicam and both more effective than the positive control (P < 0.0001). Flunixin meglumine administered animals displayed lower cortisol levels to that of meloxicam treated cows and both more effective than the positive control (P = 0.0008). The cows treated with meloxicam withstood a greater force created by mechanical nociception threshold than cows administered flunixin meglumine and both more effective than the positive control (P = 0.0008). The impact of NSAID use as a therapeutic treatment of lameness is insurmountable in combatting welfare concerns in the dairy industry.
PSIII-8 Genomic prediction of reproductive performance of commercial sows in health challenged herds
Authors: Scanlan Cassandra , Canada PigGen , Mote Benny , Willson Philip , Harding John C S , Plastow Graham S , Dekkers Jack C M , Serão Nick V L
Published: July 2019

The objective of this study was to perform genomic predictions for reproductive performance of sows under natural health challenge. Reproductive performance (1 to 4 parities) and genotype (~40K SNPs) were available for 2,604 crossbred sows, for a total of 7,635 farrowing records. Animals from 17 high-health multipliers from 7 breeding companies (PigGen Canada) were shipped to 23 commercial farms with recent history of common infectious diseases. Gilts entered farms with an average of 53 animals per contemporary group (CG). Traits included total number of piglets: born (TB), born alive (NBA), stillborn (SB), mummified (MUM), born dead (NBD), and weaned (NW). Genomic predictions were performed using Bayes-B (pi=0.995) with a seven-fold cross-validation using each company in turn for validation and the others for training. The model included the effects of CG (fixed) and SNP (random), and net number of fosters (covariate) for NW. Genomic predictions were done for animal lifetime performance (sum performance of parities) for each trait and using first parity performance as the training set to predict subsequent parity performance. Accuracy was calculated as the weighted average correlation between GEBV and adjusted phenotype across validation sets divided by the square root of heritability. Lifetime performance accuracies were low to moderate, ranging from 0.11 (TB) to 0.45 (NBD). Accuracies using parity 1 to predict subsequent performance were low, ranging from -0.07 (SB in parity 3) to 0.19 (NBD in parity 2), with average accuracies per trait ranging from 0.04 (SB) to 0.16 (NBD).Although most accuracies were low, the moderately high accuracies for some lifetime performance shows that genomic prediction can be used to improve performance under natural health challenge in sows. We appreciate the financial support of PigGen Canada, Canadian Swine Health Board, Genome Alberta and Swine Innovation Porc, and the late Dr. Stephen Bishop for his scientific contributions.
PSIII-3 Genes and functions associated with tolerance to fescue toxicosis in Angus cows
Authors: Khanal Piush , Sanglard Leticia Pereira , Mayberry Kyle , Sommer Jeffrey , Poore Matthew H , Poole Daniel H , Serão Nick V L
Published: July 2019

The objective of this study was to identify differentially expressed genes (DEG) and functions associated with tolerance to fescue toxicosis (FT). Forty pregnant purebred Angus cows were selected based on their growth at two locations in North Carolina (Butner Beef Cattle Field Laboratory, BBFCL; Upper Piedmont Research Station, UPRS) and classified as either high tolerant (HT) or low tolerant (LT) to FT with 20 cows in each group balanced by location. Blood samples were collected on weeks 1, 5, 9, and 13 for RNA sequencing. Counts were analyzed using a negative binomial model including the effects of genetic group, location, time, all possible interactions of these effects, flow cell, covariate of RNA integrity number, and normalized library size as offset. Genotype-by-location-by-time interaction was evident with a high number (4,453) of DEG (q-value<0.1) between genetic groups on week 5 at UPRS compared to all other possible interactions. So further analyses were focused on week 5 at UPRS. The most significant upregulated genes in LT and HT animals were ENPP6 and MESP2, respectively, with log2 fold changes of 1.90 [95% confidence interval = 0.89, 2.92] q-value=0.005) and 0.91 [0.35, 1.47] (q-value=0.01), respectively. Other top 5 upregulated genes for HT animals were CTBS, CLDN19, SPDYC, HEYL, and SDC2, and for LT animals were OLIG1, IL13, ANXA13, ENSBTAG00000024188and CXCL13. Enrichment analysis (P < 0.05) showed that DEG between genetic groups have general functions, such as metabolic, biosynthetic, and catabolic processes, as well as DNA and RNA-related functions, such as translation, transcription, and repair. These findings helped characterizing the genetic basis of tolerance to FT in cattle. In addition, we identified genes that may serve as potential biomarkers for tolerance to FT
PSIII-1 Nutritive value of expeller-pressed carinata cake for pigs
Authors: Woyengo Tofuko A
Published: July 2019

Carinata (Brasicca carinata) is an oilseed crop of brassica family that is grown for production of oil for biofuel industry. Expeller-pressed carinata cake (EPCAR), a co-product from carinata seed oil extraction plants, is available for livestock feeding. However, nutritive value of EPCAR for pigs has not been reported. Objective of this study was to determine standardized ileal digestibility (SID) of amino acids (AA) and net energy value of EPCAR for growing pigs. Eight ileal-cannulated barrows (initial BW = 33 kg) were fed 3 diets in a replicated 3 × 3 Latin square design with 2 added columns to give 8 replicates/diet. The diets were cornstarch-based, containing expeller-pressed canola cake (EPCAN) or EPCAR as sole protein source, and N-free. The EPCAN was included in the study for comparison because it is derived from canola, which is the most widely used crop of Brassica family for oil production. Digestibility of AA in feedstuffs was determined by the direct method. Energy digestibility in EPCAN and EPCAR was determined by difference from the N-free diet. On DM basis, EPCAN and EPCAR contained 39.6 and 50.2% CP, 20.7 and 26.8% NDF, 16.0 and 0.88% ether extract, 2.32 and 1.82% lysine, 0.74 and 0.96% methionine, 1.63 and 1.89% threonine, and 0.50 and 0.64% tryptophan, respectively. The EPCAN compared with EPCAR, had greater (PPP = 0.078) in SID of threonine. The EPCAN had greater (P < 0.05) net energy value than EPCAN (2,082 vs. 1,576 kcal/kg DM). In conclusion, EPCAR lower energy value than EPCAN. However, EPCAR had greater SID of most AA than EPCAN; therefore, EPCAR can serve as alternative oilseed co-product feedstuff for pigs.
PSIV-9 Effect of carbohydrases on growth performance, nutrient digestibility, blood profiles and fecal microbiota in weanling pigs fed reduced energy diet
Authors: Ahn Jemin , Ao Xiang , Sun Haoyang , Kim In Ho
Published: July 2019

This study was conducted to evaluate the effects of carbohydrases on growth performance, nutrient digestibility, blood profiles and fecal microbiota in weanling pigs fed reduced energy diet. A total of 160 cross-bred [(Yorkshire × Landrace) × Duroc)] weanling pigs with an initial BW of 7.23 ± 1.08 kg were used in this 42-day trial. Pigs were randomly allocated in 1 of the following 4 dietary treatments: (1) PC, positive diet (NE 2,545 kcal/kg); (2) NC, negative diet (NE 2470 kcal/kg); (3) NC1, NC + 0.1% carbohydrases; (4) NC2, NC + 0.2% carbohydrases based on the sex and BW. There were 8 replications (pens) per treatment with 5 pigs per pen. During d 0-7, pigs fed NC diet had lower (P < 0.05) average daily gain (ADG) and average daily feed intake (ADFI) than those fed PC and NC2 diets. NC group reduced (P < 0.05) ADG throughout the experiment compared with PC and NC2 groups. NC group reduced (P < 0.05) the digestibility of dry matter, nitrogen and gross energy. Taken together, reduced energy diet caused detrimental effects on growth performance and nutrient digestibility in weanlings, while the addition of carbohydrases (0.2%) can partially counteract these negative effects.
PSIV-1 Apparent ileal and total tract digestibility of energy and carbohydrates in hybrid rye and other cereal grains fed to growing pigs
Authors: McGhee Molly L , Stein Hans H
Published: July 2019

An experiment was conducted to test the hypothesis that apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of energy, starch, and total dietary fiber (TDF), and the concentration of ME in 2 hybrids of rye (Bono and Brasetto) are not different from values for barley, wheat, corn, and sorghum. Twenty-four ileal cannulated barrows (28.1 ± 3.0 kg) were placed in metabolism crates and randomly allotted to a 2-period experimental design with 6 diets and 4 replicate pigs in each 13-d period. Diets consisted of 97% of each grain, and no pig received the same diet twice. Urine and feces were collected for 4 d after 5 d of diet adaptation, and ileal digesta were collected on d 12 and 13 of each period. Data were analyzed using the Mixed procedure of SAS with the fixed effect of diet and the random effects of pig and period. The ME (DM basis) was greatest (P < 0.05) in corn and wheat, and least (P < 0.05) in barley, and the ME in Bono and Brasetto rye was 3,499 and 3,459 kcal/kg DM, respectively (Table 1). In all grains, the AID of starch was greater than 90%, and the ATTD of starch was nearly 100%. The AID of TDF was reduced (P < 0.05) in Bono rye compared with all other cereal grains, but the ATTD of TDF was greater (P < 0.05) in Bono and Brasetto rye than in the other grains. In conclusion, the current data indicate that rye results in reduced pre-cecal absorption of energy compared with wheat, corn, and sorghum, but hindgut fermentation of fiber is greater for pigs fed rye. The ME in hybrid rye is not different from values for barley and sorghum, but less than in wheat and corn.
PSIV-14 Effect of xylanase and xylo-oligosaccharides on growth performance and intestinal microbiota in piglets fed a corn-soybean meal based diet
Authors: Cordero Gustavo , Merriman Laura , de Brito Alexandre Barbosa , da Silva Caio Abercio , Callegari M A , González-Ortiz Gemma
Published: July 2019

Xylanases are commonly used to improve nutrient digestibility and performance in pig diets. Xylo-oligosaccharides (XOS) are degraded in the gut primarily by Bifidobacteria, leading to an increase in butyrate production. The objective of this study was to determine the effect of a blend of a xylanase and XOS (Signis, AB Vista) on growth performance and intestinal microbiota profile of weaning piglets. A total of 168 mixed sex PIC weaned piglets weighting between 4.5 and 6.7 kg (5.3 kg average weight) were allocated to 28 pens with 6 animals each and 14 pens per diet blocked by initial weight. Diets consisted of a control (based on corn-SBM) without and with 100 g/tonne Signis. The mash diets were fed over 2 phases: pre-starter (6-11 kg) and starter (11-25 kg). Feed samples were collected for xylanase activities analyses, and results were as expected. Liveability, average daily gain and feed intake were recorded, and feed conversion ratio (FCR) calculated. Colon digesta samples were collected after 6 weeks of Signis supplementation for bacterial identification through 16S rRBA rRNA (V3-V4) sequencing. Results are presented as the relative operational taxonomic units (OTU) values for each bacteria sequenced. Overall, inclusion of Signis reduced (P = 0.05) FCR by 9 points (1.79 vs. 1.88; SEM=0.025) at 42 days post-weaning. No differences in the OTU values were observed between treatments, with Firmicutes making up the largest proportion of the colon microbiota, followed by Actinobacteria and Proteobacteria. Bacteroidetes tended (P < 0.10) to be higher in the Signis group (0.06 vs 0.22; SEM=0.058). Piglets supplemented with Signis had a trend (P < 0.10) to have higher concentration of uncultured Clostridium spp, which are involved in the fermentation of fiber. This suggests that Signis stimulates the development of a fibrolytic microbiome and thus piglet performance.
PSIV-25 Development of an indirect calorimetry system to determine heat production in individual lactating sows
Authors: Johnson Jay S , Zhang Sai , Morello Gabriela M , Maskal Jacob M , Trottier Nathalie L
Published: July 2019

Determining total heat production (THP) in individual sows and litters can be difficult and often requires the use of multiple animals to generate data on a per room basis. These systems may be costly to construct precluding their use by many researchers. The study objective was to develop a low cost indirect calorimetry system to determine THP in individual lactating sows and litters. Six indirect calorimeters were constructed to house 1 sow and litter in a crate throughout farrowing and a 21-d lactation period. Chamber accuracies for O2 and CO2 were evaluated by ethanol combustion. One-week pre-farrowing, 6 pregnant multiparous sows (parity 2.9 ± 0.9; 218.3 ± 38.6 kg BW) were housed individually in each farrowing crate and maintained in thermoneutral conditions (20.9 ± 2.6°C and 43.7 ± 18.6% relative humidity) throughout lactation. On lactation d 4, 8, 14, and 18, indirect calorimetry was performed on all sows and their litters, as well as 2 piglets from a sentinel litter to determine THP. Sentinel piglet data were used to estimate THP for the sows independent of the litter. Sow + litter THP (kcal/h) increased (P = 0.01; 16.6%) on d 8 compared to d 4 and was greater (27.3%) on d 14 and d 18 compared to d 4 and d 8. Sow THP was greater (P = 0.01) on d 8 (401.19 ± 17.15 kcal/h) and d 14 (430.79 ± 12.42 kcal/h) compared to d 4 (346.16 ± 16.62 kcal/h), and was greater on d 14 compared to d 8 and on d 18 (386.16 ± 20.02 kcal/h) compared to d 14. In summary, this cost-effective system can allow researchers to accurately evaluate THP in individual lactating sows and their litters
PSV-19 Effect of yeast bioactive compounds on the reproductive performance of sows
Authors: Garcia Ricardo M , Chaves Rhuan , Arruda Gabriel , Resende Maíra , Barbosa Jéssica , Oliveira Eduardo , Cantarelli Vinícius S
Published: July 2019

Prebiotics has been used in sow diets as an alternative to minimize the impacts of hyperprolificity, such as variability coefficient and poor litter performance. However, the additive inclusion is generally high and costly. The objective of this study was to verify the effect of the low inclusion of yeast bioactive compounds (YBC), in gestation and lactation diets, on sows reproductive performance. Five hundred sows were assigned in two treatments in a block design considering parity as a random factor, using each sow as an experimental unit with for gestation (n = 500) and lactation period (n = 80). The treatments were control diet (CON) and diet with 0.036% of YBC inclusion (YBC). Back fat thickness was measured at 30 and 70 days of gestation, at farrowing day, at 14 days of lactation and weaning day. Sows were weighted at the farrowing barn entry day and at weaning. The corporal mobilization was accessed following the equation: Body change = Weight at weaning - [Weight at lactation entry - (Litter weight at birth + Placenta weight)]. After cross-fostering and at weaning the litters were weighed to calculate the daily weight gain. Differences were considered significant when p ≤ 0.05 and tendencies were considered when p > 0.05 and p < 0.10. No difference was found regarding the reproductive performance (total born, born alive, mummified and stillborns) (P > 0.05). YBC tended to have greater litter final weight than CON group, 59.08 kg versus 56.70 kg (P = 0.057). Backfat thickness tended to be lower (5.20%) in YBC (16.03 mm) than CON group (16.91 mm) during gestation period (P = 0.075). However, at weaning day no difference for backfat thickness was found between treatments (P > 0.05). YBC had lower loss weight during lactation than CON group, 0.0 kg versus 4.99 kg, respectively (P = 0.011). Low inclusion of YBC enhances litter performance and improves body condition of lactating sows.
PSV-22 Effects of different fat sources and vitamin E levels/isoforms on growth performance, antioxidant status, and fatty acid profile in the liver of pigs grown to 150 kg
Authors: Wang Ding , Jang Young Dal , Rentfrow G K , Monegue H J , Azain M J , Lindemann Merlin D
Published: July 2019

The study objective was to assess the contribution of fat source and vitamin E (VE) supplementation on growth performance, antioxidant status, and fatty acid profile in the liver of pigs at heavy slaughter weight (148.09 ±1.64 kg). A total of 72 individually-fed pigs (36 barrows, 36 gilts; 28.55 ±1.16 kg) were randomly assigned to 12 dietary treatments in a 2 × 6 factorial arrangement. Fat treatments were tallow and corn-oil. The VE treatments included four levels of α-tocopheryl-acetate (ATA; 11, 40, 100, and 200 ppm) and two levels of mixed tocopherols (primarily γ-tocopherol; 40 and 100 ppm). Liver samples were collected at slaughter. Data analysis were performed by ANOVA using GLM in SAS. No effect of dietary fat sources, isoforms of VE, and levels of γ-tocopherol were detected on growth performance or liver antioxidant status (GSH, GSSG, SOD, and MDA). Increasing dietary ATA linearly increased overall ADG (0.98, 1.02, 1.02, and 1.08 kg/day; P = 0.02) from 28 to 150 kg; overall ADG of pigs fed γ-tocopherol-diets was 1.07 and 1.04 kg/day, respectively. An interaction between fat sources and ATA was observed on cumulative ADG during Phase1-3 (28-100 kg; P = 0.04) and Phase1-4 (28-125 kg; P = 0.03) wherein pigs fed corn-oil-diet, but not tallow-diet, had increased ADG with increased dietary ATA. Increasing dietary ATA quadratically increased SOD activity (P < 0.05; highest at 100 ppm), and quadratically decreased MDA content (P < 0.05; lowest at 40 ppm) in the liver. Liver fatty acid profile was affected by fat source (P < 0.05) but not VE treatment, pigs fed corn-oil-diets had less SFA (34.23 vs. 35.45%; P = 0.02) and MUFA (11.98 vs. 18.38%; P < 0.0001), but more PUFA (49.14 vs. 40.78%; P < 0.0001) than pigs fed tallow-diets in the liver. Beneficial effects of dietary VE supplementation were observed on growth performance and antioxidant status of pigs under conditions of this study.