bluuebunny/crossref_metadata_2025 huggingface dataset:
Marine Microalgal Products with Activities against Age-Related Cardiovascular Diseases
Authors: Yurika Nova , Montuori Eleonora , Lauritano Chiara
Published: May 2024

Heart disease is one of the leading causes of death worldwide, and it is estimated that 17.9 million people die of it each year. The risk factors for cardiovascular diseases are attributable to an unhealthy and sedentary lifestyle, poor nutrition, stress, genetic predisposition, diabetes, obesity, and aging. Marine microalgae have been the subject of numerous studies for their potential activity against several human diseases. They produce a plethora of primary and secondary metabolites such as essential nutrients, vitamins, pigments, and omega-3 fatty acid. Many of these molecules have antioxidant properties and have been shown to play a role in the prevention of heart diseases. The aim of this review is to summarize recent studies on the discovery of marine microalgal compounds and bioactivities for cardiovascular diseases, including in vitro and in vivo studies, showing and discussing recent discoveries and trends. The most promising results were found for microalgal polysaccharides, peptides and carotenoids. In conclusion, the overall data summarized here show that microalgae-based supplementation has the potential to improve age-related cardiovascular diseases and we expect more clinical studies in the future.
Uterine Leiomyomas with Specific Histology Features of Two Fumarate Hydratase/Succinate Dehydrogenase-Deficient Tumors: A Double Case Report
Authors: Jovanović Ljubiša , Milenković Svetlana , Andrić Luka , Stefanović Radomir , Milošević Branislav , Micić Jelena , Pilić Igor , Beleslin Aleksandra , Mihaljević Olga , Dokić Milan
Published: May 2024

Background and Objectives: Mutations in succinate dehydrogenase (SDH) and fumarate hydratase (FH) give rise to various familial cancer syndromes, with these alterations being characteristic of certain types of histomorphologically specific leiomyomas that hold significant predictive value. Materials and Methods: This study presents two cases of uterine leiomyomas exhibiting rare histomorphological and genetic characteristics, which are crucial for prognosis and further treatment. Results: Distinct histopathological features such as marked nuclear atypia, intracellular eosinophilic globules, and abnormal intratumoral vessels raise suspicion for specific leiomyoma subtypes, which carry predictive significance for additional hereditary cancer syndromes. Immunohistochemical analysis confirmed FH/SDH deficiency in both patients, who underwent careful follow-up. Conclusions: This study describes two cases involving unusual leiomyomas, the histopathological characteristics of which may easily go unrecognized. These features hold predictive significance because their specific mutations point to additional hereditary cancer syndromes, highlighting the need for further examinations.
Emotion Processing in Peripheral Neuropathic Pain: An Observational Study
Authors: Isoardo Gianluca , Adenzato Mauro , Ciullo Stefano , Fontana Elena , Stura Ilaria , Migliaretti Giuseppe , Titolo Paolo , Matteoni Enrico , Calvo Andrea , Laino Federica , Palumbo Francesca , Ardito Rita B.
Published: May 2024

Background: In clinical practice, the implementation of tailored treatment is crucial for assessing the patient’s emotional processing profile. Here, we investigate all three levels of analysis characterizing emotion processing, i.e., recognition, representation, and regulation, in patients with peripheral neuropathic pain (PNP). Methods: Sixty-two patients and forty-eight healthy controls underwent quantitative sensory testing, i.e., psychophysical tests to assess somatosensory functions such as perception of cold (CDT), heat-induced pain (HPT), and vibration (VDT), as well as three standardized tasks to assess emotional processing: (1) the Ekman 60-Faces Test (EK-60F) to assess recognition of basic facial emotions, (2) the Reading the Mind in the Eyes Test (RME) to assess the ability to represent the feelings of another person by observing their eyes, and (3) the 20-item Toronto Alexithymia Scale (TAS-20) to assess emotional dysregulation, i.e., alexithymia. Results: General Linear Model analysis revealed a significant relationship between left index finger VDT z-scores in PNP patients with alexithymia. The RME correlated with VDT z-scores of the left little finger and overall score for the EK-60F. Conclusions: In patients with PNP, emotion processing is impaired, which emphasizes the importance of assessing these abilities appropriately in these patients. In this way, clinicians can tailor treatment to the needs of individual patients.
Residents or Tourists: Is the Lactating Mammary Gland Colonized by Residential Microbiota?
Authors: Xu Ruomei , McLoughlin Grace , Nicol Mark , Geddes Donna , Stinson Lisa
Published: May 2024

The existence of the human milk microbiome has been widely recognized for almost two decades, with many studies examining its composition and relationship to maternal and infant health. However, the richness and viability of the human milk microbiota is surprisingly low. Given that the lactating mammary gland houses a warm and nutrient-rich environment and is in contact with the external environment, it may be expected that the lactating mammary gland would contain a high biomass microbiome. This discrepancy raises the question of whether the bacteria in milk come from true microbial colonization in the mammary gland (“residents”) or are merely the result of constant influx from other bacterial sources (“tourists”). By drawing together data from animal, in vitro, and human studies, this review will examine the question of whether the lactating mammary gland is colonized by a residential microbiome.
In-Host Flat-like Quasispecies: Characterization Methods and Clinical Implications
Authors: Gregori Josep , Colomer-Castell Sergi , Ibañez-Lligoña Marta , Garcia-Cehic Damir , Campos Carolina , Buti Maria , Riveiro-Barciela Mar , Andrés Cristina , Piñana Maria , González-Sánchez Alejandra , Rodriguez-Frias Francisco , Cortese Maria Francesca , Tabernero David , Rando-Segura Ariadna , Pumarola Tomás , Esteban Juan Ignacio , Antón Andrés , Quer Josep
Published: May 2024

The repeated failure to treat patients chronically infected with hepatitis E (HEV) and C (HCV) viruses, despite the absence of resistance-associated substitutions (RAS), particularly in response to prolonged treatments with the mutagenic agents of HEV, suggests that quasispecies structure may play a crucial role beyond single point mutations. Quasispecies structured in a flat-like manner (referred to as flat-like) are considered to possess high average fitness, occupy a significant fraction of the functional genetic space of the virus, and exhibit a high capacity to evade specific or mutagenic treatments. In this paper, we studied HEV and HCV samples using high-depth next-generation sequencing (NGS), with indices scoring the different properties describing flat-like quasispecies. The significance of these indices was demonstrated by comparing the values obtained from these samples with those from acute infections caused by respiratory viruses (betacoronaviruses, enterovirus, respiratory syncytial viruses, and metapneumovirus). Our results revealed that flat-like quasispecies in HEV and HCV chronic infections without RAS are characterized by numerous low-frequency haplotypes with no dominant one. Surprisingly, these low-frequency haplotypes (at the nucleotide level) exhibited a high level of synonymity, resulting in much lower diversity at the phenotypic level. Currently, clinical approaches for managing flat-like quasispecies are lacking. Here, we propose methods to identifying flat-like quasispecies, which represents an essential initial step towards exploring alternative treatment protocols for viruses resistant to conventional therapies.
A Review: Microbes and Their Effect on Growth Performance of Litopenaeus vannamei (White Leg Shrimps) during Culture in Biofloc Technology System
Authors: Raza Bilal , Zheng Zhongming , Zhu Jinyong , Yang Wen
Published: May 2024

In the modern era of Aquaculture, biofloc technology (BFT) systems have attained crucial attention. This technology is used to reduce water renewal with the removal of nitrogen and to provide additional feed. In BFT, microorganisms play a crucial role due to their complex metabolic properties. Pathogens can be controlled through multiple mechanisms using probiotics, which can promote host development and enhance the quality of the culture environment. During culturing in a biofloc technology system, the supplementation of microalgae and its accompanying bacteria plays a beneficial role in reducing nitrogenous compounds. This enhances water quality and creates favorable environmental conditions for specific bacterial groups, while simultaneously reducing the dependency on carbon sources with higher content. The fluctuations in the bacterial communities of the intestine are closely associated with the severity of diseases related to shrimp and are used to evaluate the health status of shrimp. Overall, we will review the microbes associated with shrimp culture in BFT and their effects on shrimp growth. We will also examine the microbial impacts on the growth performance of L. vannamei in BFT, as well as the close relationship between probiotics and the intestinal microbes of L. vannamei.
Probiotics in the New Era of Human Milk Oligosaccharides (HMOs): HMO Utilization and Beneficial Effects of Bifidobacterium longum subsp. infantis M-63 on Infant Health
Authors: Wong Chyn Boon , Huang Huidong , Ning Yibing , Xiao Jinzhong
Published: May 2024

A healthy gut microbiome is crucial for the immune system and overall development of infants. Bifidobacterium has been known to be a predominant species in the infant gut; however, an emerging concern is the apparent loss of this genus, in particular, Bifidobacterium longum subsp. infantis (B. infantis) in the gut microbiome of infants in industrialized nations, underscoring the importance of restoring this beneficial bacterium. With the growing understanding of the gut microbiome, probiotics, especially infant-type human-residential bifidobacteria (HRB) strains like B. infantis, are gaining prominence for their unique ability to utilize HMOs and positively influence infant health. This article delves into the physiology of a probiotic strain, B. infantis M-63, its symbiotic relationship with HMOs, and its potential in improving gastrointestinal and allergic conditions in infants and children. Moreover, this article critically assesses the role of HMOs and the emerging trend of supplementing infant formulas with the prebiotic HMOs, which serve as fuel for beneficial gut bacteria, thereby emulating the protective effects of breastfeeding. The review highlights the potential of combining B. infantis M-63 with HMOs as a feasible strategy to improve health outcomes in infants and children, acknowledging the complexities and requirements for further research in this area.
Exploring Gut Microbiota Alterations with Trimethoprim-Sulfamethoxazole and Dexamethasone in a Humanized Microbiome Mouse Model
Authors: Green George B. H. , Cox-Holmes Alexis N. , Backan Olivia , Valbak Olivia , Potier Anna Claire E. , Chen Dongquan , Morrow Casey D. , Willey Christopher D. , McFarland Braden C.
Published: May 2024

Along with the standard therapies for glioblastoma, patients are commonly prescribed trimethoprim-sulfamethoxazole (TMP-SMX) and dexamethasone for preventing infections and reducing cerebral edema, respectively. Because the gut microbiota impacts the efficacy of cancer therapies, it is important to understand how these medications impact the gut microbiota of patients. Using mice that have been colonized with human microbiota, this study sought to examine how TMP-SMX and dexamethasone affect the gut microbiome. Two lines of humanized microbiota (HuM) Rag1−/− mice, HuM1Rag and HuM2Rag, were treated with either TMP-SMX or dexamethasone via oral gavage once a day for a week. Fecal samples were collected pre-treatment (pre-txt), one week after treatment initiation (1 wk post txt), and three weeks post-treatment (3 wk post txt), and bacterial DNA was analyzed using 16S rRNA-sequencing. The HuM1Rag mice treated with TMP-SMX had significant shifts in alpha diversity, beta diversity, and functional pathways at all time points, whereas in the HuM2Rag mice, it resulted in minimal changes in the microbiome. Likewise, dexamethasone treatment resulted in significant changes in the microbiome of the HuM1Rag mice, whereas the microbiome of the HuM2Rag mice was mostly unaffected. The results of our study show that routine medications used during glioblastoma treatment can perturb gut microbiota, with some microbiome compositions being more sensitive than others, and these treatments could potentially affect the overall efficacy of standard-of-care therapy.
Sodium Polyoxotungstate Inhibits the Replication of Influenza Virus by Blocking the Nuclear Import of vRNP
Authors: Li Zhuogang , Duan Yuanyuan , Yu Yang , Su Yue , Zhang Mingxin , Gao Yarou , Jiang Lefang , Zhang Haonan , Lian Xiaoqin , Zhu Xingjian , Ke Jiaxin , Peng Qun , Chen Xulin
Published: May 2024

Both pandemic and seasonal influenza are major health concerns, causing significant mortality and morbidity. Current influenza drugs primarily target viral neuraminidase and RNA polymerase, which are prone to drug resistance. Polyoxometalates (POMs) are metal cation clusters bridged by oxide anions. They have exhibited potent anti-tumor, antiviral, and antibacterial effects. They have remarkable activity against various DNA and RNA viruses, including human immunodeficiency virus, herpes simplex virus, hepatitis B and C viruses, dengue virus, and influenza virus. In this study, we have identified sodium polyoxotungstate (POM-1) from an ion channel inhibitor library. In vitro, POM-1 has been demonstrated to have potent antiviral activity against H1N1, H3N2, and oseltamivir-resistant H1N1 strains. POM-1 can cause virion aggregation during adsorption, as well as endocytosis. However, the aggregation is reversible; it does not interfere with virus adsorption and endocytosis. Our results suggest that POM-1 exerts its antiviral activity by inhibiting the nuclear import of viral ribonucleoprotein (vRNP). This distinct mechanism of action, combined with its wide range of efficacy, positions POM-1 as a promising therapeutic candidate for influenza treatment and warrants further investigation.
Raman Scattering Enhancement through Pseudo-Cavity Modes
Authors: Caligiuri Vincenzo , Nucera Antonello , Patra Aniket , Castriota Marco , De Luca Antonio
Published: May 2024

Raman spectroscopy plays a pivotal role in spectroscopic investigations. The small Raman scattering cross-section of numerous analytes, however, requires enhancement of the signal through specific structuring of the electromagnetic and morphological properties of the underlying surface. This enhancement technique is known as surface-enhanced Raman spectroscopy (SERS). Despite the existence of various proposed alternatives, the approach involving Fabry–Pérot cavities, which constitutes a straightforward method to enhance the electromagnetic field around the analyte, has not been extensively utilized. This is because, for the analyte to experience the maximum electric field, it needs to be embedded within the cavity. Consequently, the top mirror of the cavity will eventually shield it from the external laser source. Recently, an open-cavity configuration has been demonstrated to exhibit properties similar to the classic Fabry–Pérot configuration, with the added advantage of maintaining direct accessibility for the laser source. This paper showcases how such a simple yet innovative configuration can be effectively utilized to achieve remarkable Raman enhancement. The simple structure, coupled with its inexpensive nature and versatility in material selection and scalability, makes it an ideal choice for various analytes and integration into diverse Raman apparatus setups.
MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression
Authors: Lozano-Velasco Estefanía , Garcia-Padilla Carlos , Carmona-Garcia Miguel , Gonzalez-Diaz Alba , Arequipa-Rendon Angela , Aranega Amelia E. , Franco Diego
Published: May 2024

Transcriptional regulation constitutes a key step in gene expression regulation. Myocyte enhancer factor 2C (MEF2C) is a transcription factor of the MADS box family involved in the early development of several cell types, including muscle cells. Over the last decade, a novel layer of complexity modulating gene regulation has emerged as non-coding RNAs have been identified, impacting both transcriptional and post-transcriptional regulation. microRNAs represent the most studied and abundantly expressed subtype of small non-coding RNAs, and their functional roles have been widely documented. On the other hand, our knowledge of the transcriptional and post-transcriptional regulatory mechanisms that drive microRNA expression is still incipient. We recently demonstrated that MEF2C is able to transactivate the long, but not short, regulatory element upstream of the miR-23a-miR-27a-miR-24-2 transcriptional start site. However, MEF2C over-expression and silencing, respectively, displayed distinct effects on each of the miR-23a-miR-27a-miR-24-2 mature cluster members without affecting pri-miRNA expression levels, thus supporting additional MEF2C-driven regulatory mechanisms. Within this study, we demonstrated a complex post-transcriptional regulatory mechanism directed by MEF2C in the regulation of miR-23a-miR-27a-miR-24-2 cluster members, distinctly involving different domains of the MEF2C transcription factor and the physical interaction with pre-miRNAs and Ksrp, HnRNPa3 and Ddx17 transcripts.
Association between Dietary Quality and Non-Alcoholic Fatty Liver Disease in Korean Adults: A Nationwide, Population-Based Study Using the Korean Healthy Eating Index (2013–2021)
Authors: Baek Seong-Uk , Kim Taeyeon , Lee Yu-Min , Won Jong-Uk , Yoon Jin-Ha
Published: May 2024

This study explored the relationship between the Korean Healthy Eating Index (KHEI) and non-alcoholic fatty liver disease (NAFLD). This cross-sectional study included 34,174 Korean adults. The KHEI was composed of three subcomponents (adequacy, moderation, and energy balance) and calculated based on a 24 h dietary recall. The total score ranged from 0 to 100, with a higher score indicating a greater adherence to the Korean dietary guidelines. The total KHEI scores were categorized into quartiles (Q1–Q4). NAFLD was classified using the hepatic steatosis index. Logistic regressions were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs). The adjusted OR (95% CI) of NAFLD was 0.95 (0.87–1.03) for the Q2 group, 0.90 (0.83–0.98) for the Q3 group, and 0.79 (0.72–0.87) for the Q4 group, compared with the Q1 group. Among the subcomponents of the KHEI, individuals with high scores in the adequacy component, characterized by an abundant consumption of fruits, vegetables, and dairy products, exhibited the most pronounced association with NAFLD. A higher KHEI score was negatively associated with NAFLD in Korean adults. Therefore, the promotion of healthy dietary patterns can play a beneficial role in the prevention or management of NAFLD.
Researching Gender and Disasters of Natural Origin: Ethical Challenges
Authors: Dema Moreno Sandra , Alonso Moro María Teresa , Cocina Díaz Virginia
Published: May 2024

Ethical issues are very relevant in the field of women’s, gender and/or feminist studies. The aim of this article is to highlight the ethical challenges faced by the authors in their research process, with specific reference to two projects on gender and disasters in which they have been involved. In general, we try to avoid sexist bias throughout the complete research process, from the definition of the objectives themselves to the methodology design, where we ensure diversity in the selection of participants in order to take into consideration the variety of voices present in society, especially those of women. Also, when developing our research, we take into account the power relationships involved, both between those who participate in the fieldwork and with the researchers themselves. To counteract the effects of such relations, we have considered people’s wellbeing and the humanization of the whole process. Finally, when it comes to the dissemination of the results and their transfer to society at large, we follow the same principles and actively integrate the people involved. Considering these issues benefits the research process and makes the resultant knowledge more ethical and socially useful, in addition to promoting more egalitarian gender relations.
Mechanical Assessment in Atherosclerosis Based on Photoacoustic Viscoelasticity Imaging
Authors: Zhang Xingchao , Shi Xiaohan , Wu Hui , Bai Caixun , Xiu Junshan , Zhao Yue
Published: May 2024

Early identification of vulnerable plaques is a major challenge in diagnosis and assessment of atherosclerosis. In atherosclerotic plaque development, the proportion change in components caused plaque mechanical property change and induced plaque rupture. In this paper, a photoacoustic viscoelasticity imaging (PAVEI) technique was proposed to measure the viscosity–elasticity ratio of atherosclerotic plaque and evaluated for the potential in characterizing vulnerable plaques. Apolipoprotein E-knockout mice fed with a high-fat/high-cholesterol diet were chosen as the atherosclerotic model. Plaque component phantoms were examined to demonstrate the high efficiency of PAVEI in detecting the proportion change in components compared to single elasticity or viscosity detection. Finally, atherosclerotic plaques from mice aortas at different stages were imaged by PAVEI, which provided an insight into the compositional and functional characterization of vulnerability plaques and suggested its potential applications in the identification of high-risk plaques.
Development of New Series of Certified Reference Materials for Ferrosilicon Magnesium Alloys
Authors: Kostrzewa Justyna , Anyszkiewicz Jacek , Gorewoda Tadeusz , Jamroz Ewa , Blandhol Kjell , Guldhav Alf Yngve , Knapik Magdalena , Charasińska Jadwiga , Jakóbik-Kolon Agata
Published: May 2024

This paper presents a practical approach to the production of certified reference materials (CRMs) for ferrosilicon magnesium alloys. These new CRMs are predicted to be used in fast X-ray fluorescence spectrometry (XRF) analysis, which does not require sample digestion and does not result in the production of acidic sewage and emissions, contrary to the classical and instrumental techniques currently used in laboratories. Four new certified reference materials (CRMs) were developed to fill the gap in the reference materials market and ensure fast and traceable analyses. The materials were produced with an industrial process and then homogenized and mixed to achieve the required compositions and level of homogeneity. The homogeneity was determined using specially developed analytical methods and confirmed statistically by ANOVA. Additionally, the results of the tests show the short- and long-term stabilities of the new materials. The certified values for specific element contents were determined in interlaboratory tests. All results were assessed statistically for outliers. The results from three or more independent and different analytical methods were used for the calculations. In parallel homogeneity, the stability, and characterization standard uncertainties were calculated and used in the estimation of the final expanded uncertainties of the certified values. Finally, four new CRMs were assisted with the proper certificates according to ISO standards.
Caring for–Caring about: Negotiations of Values in Pastoral Care
Authors: Christoffersen Mikkel Gabriel , Haußmann Annette Daniela , Austad Anne
Published: May 2024

The term “care” in pastoral care means caring for others. Yet those who care for others in pastoral conversations can also be defined existentially as people who care about the world, that is, people who hold values. This article explores how caring for and caring about commence in pastoral practice, with special attention paid to conflicts of values in pastoral conversations. The article proposes a typology of subjects for value conflicts in pastoral care, and it proposes a set of strategies for navigating those conflicts. We base both proposals on an analysis of German and Norwegian verbatims, i.e., protocols of pastoral caregivers’ memories of pastoral care encounters. These verbatims highlight that while pastoral caregivers and care seekers have different roles and obligations in pastoral care, an existential encounter occurs which has its own potentials and pitfalls. Thereby, we draw attention to the necessary negotiations of values that transpire in pastoral conversations in postsecular societies.
The Performance of a Passive Autoranging Method for a Photonic Current Transducer
Authors: Fusiek Grzegorz , Mir Burhan , Niewczas Pawel
Published: May 2024

This paper reports on the testing and evaluation of a passive autoranging (AR) method designed to dynamically extend the measurement range of a photonic current transducer (PCT) to pave the way toward a realization of a combined metering- and protection-class current sensor. The PCT utilizes a current transformer (CT), a piezoelectric transducer (PZT), and a fiber Bragg grating (FBG) to enable current measurement at multiple points in an electrical power network whereby multiple sensors are deployed and interrogated serially using a single optical fiber. The autoranging technique relies on incorporating static MOSFET switches to instantaneously short individual serially connected CT burdens in response to a measured current magnitude exceeding pre-set thresholds. The AR circuit switching events produce distinctive signal features that are used by the proposed switching algorithm to apply appropriate scaling factors to reconstruct the measured current from the optical signal. It is shown through laboratory experiments that the AR circuit correctly reacts to pre-set burden current thresholds of 130% of the nominal value and 22 times the nominal value, signifying its “metering” and “protection” range boundaries. The circuit reaction time is below 4 ms, rendering it suitable for standard power system protection purposes. Moreover, the operation of the AR circuit is demonstrated for burden currents of up to 100 A for over 1 s, satisfying a test procedure for the secondary CT circuit, as required by some power system operators. It is demonstrated that the proposed switching algorithm allows for a correct reconstruction of the burden currents from the optical signal acquired by the FBG interrogator, offering the potential to realize a dual-class optical current sensor.
A Survey on Data Compression Techniques for Automotive LiDAR Point Clouds
Authors: Roriz Ricardo , Silva Heitor , Dias Francisco , Gomes Tiago
Published: May 2024

In the evolving landscape of autonomous driving technology, Light Detection and Ranging (LiDAR) sensors have emerged as a pivotal instrument for enhancing environmental perception. They can offer precise, high-resolution, real-time 3D representations around a vehicle, and the ability for long-range measurements under low-light conditions. However, these advantages come at the cost of the large volume of data generated by the sensor, leading to several challenges in transmission, processing, and storage operations, which can be currently mitigated by employing data compression techniques to the point cloud. This article presents a survey of existing methods used to compress point cloud data for automotive LiDAR sensors. It presents a comprehensive taxonomy that categorizes these approaches into four main groups, comparing and discussing them across several important metrics.
Sensor for Rapid In-Field Classification of Cannabis Samples Based on Near-Infrared Spectroscopy
Authors: Zimmerleiter Robert , Greibl Wolfgang , Meininger Gerold , Duswald Kristina , Hannesschläger Günther , Gattinger Paul , Rohm Matthias , Fuczik Christian , Holzer Robert , Brandstetter Markus
Published: May 2024

A rugged handheld sensor for rapid in-field classification of cannabis samples based on their THC content using ultra-compact near-infrared spectrometer technology is presented. The device is designed for use by the Austrian authorities to discriminate between legal and illegal cannabis samples directly at the place of intervention. Hence, the sensor allows direct measurement through commonly encountered transparent plastic packaging made from polypropylene or polyethylene without any sample preparation. The measurement time is below 20 s. Measured spectral data are evaluated using partial least squares discriminant analysis directly on the device’s hardware, eliminating the need for internet connectivity for cloud computing. The classification result is visually indicated directly on the sensor via a colored LED. Validation of the sensor is performed on an independent data set acquired by non-expert users after a short introduction. Despite the challenging setting, the achieved classification accuracy is higher than 80%. Therefore, the handheld sensor has the potential to reduce the number of unnecessarily confiscated legal cannabis samples, which would lead to significant monetary savings for the authorities.
Classification of Benign–Malignant Thyroid Nodules Based on Hyperspectral Technology
Authors: Wang Junjie , Du Jian , Tao Chenglong , Qi Meijie , Yan Jiayue , Hu Bingliang , Zhang Zhoufeng
Published: May 2024

In recent years, the incidence of thyroid cancer has rapidly increased. To address the issue of the inefficient diagnosis of thyroid cancer during surgery, we propose a rapid method for the diagnosis of benign and malignant thyroid nodules based on hyperspectral technology. Firstly, using our self-developed thyroid nodule hyperspectral acquisition system, data for a large number of diverse thyroid nodule samples were obtained, providing a foundation for subsequent diagnosis. Secondly, to better meet clinical practical needs, we address the current situation of medical hyperspectral image classification research being mainly focused on pixel-based region segmentation, by proposing a method for nodule classification as benign or malignant based on thyroid nodule hyperspectral data blocks. Using 3D CNN and VGG16 networks as a basis, we designed a neural network algorithm (V3Dnet) for classification based on three-dimensional hyperspectral data blocks. In the case of a dataset with a block size of 50 × 50 × 196, the classification accuracy for benign and malignant samples reaches 84.63%. We also investigated the impact of data block size on the classification performance and constructed a classification model that includes thyroid nodule sample acquisition, hyperspectral data preprocessing, and an algorithm for thyroid nodule classification as benign and malignant based on hyperspectral data blocks. The proposed model for thyroid nodule classification is expected to be applied in thyroid surgery, thereby improving surgical accuracy and providing strong support for scientific research in related fields.
Explainable AI: Machine Learning Interpretation in Blackcurrant Powders
Authors: Przybył Krzysztof
Published: May 2024

Recently, explainability in machine and deep learning has become an important area in the field of research as well as interest, both due to the increasing use of artificial intelligence (AI) methods and understanding of the decisions made by models. The explainability of artificial intelligence (XAI) is due to the increasing consciousness in, among other things, data mining, error elimination, and learning performance by various AI algorithms. Moreover, XAI will allow the decisions made by models in problems to be more transparent as well as effective. In this study, models from the ‘glass box’ group of Decision Tree, among others, and the ‘black box’ group of Random Forest, among others, were proposed to understand the identification of selected types of currant powders. The learning process of these models was carried out to determine accuracy indicators such as accuracy, precision, recall, and F1-score. It was visualized using Local Interpretable Model Agnostic Explanations (LIMEs) to predict the effectiveness of identifying specific types of blackcurrant powders based on texture descriptors such as entropy, contrast, correlation, dissimilarity, and homogeneity. Bagging (Bagging_100), Decision Tree (DT0), and Random Forest (RF7_gini) proved to be the most effective models in the framework of currant powder interpretability. The measures of classifier performance in terms of accuracy, precision, recall, and F1-score for Bagging_100, respectively, reached values of approximately 0.979. In comparison, DT0 reached values of 0.968, 0.972, 0.968, and 0.969, and RF7_gini reached values of 0.963, 0.964, 0.963, and 0.963. These models achieved classifier performance measures of greater than 96%. In the future, XAI using agnostic models can be an additional important tool to help analyze data, including food products, even online.
Detecting of Barely Visible Impact Damage on Carbon Fiber Reinforced Polymer Using Diffusion Ultrasonic Improved by Time-Frequency Domain Disturbance Sensitive Zone
Authors: Ma Yuqi , Li Fangyuan , Wu Jianbo , Liu Zhaoting , Xia Hui , Xu Zhaoyuan
Published: May 2024

Based on the decorrelation calculation of diffusion ultrasound in time-frequency domain, this paper discusses the repeatability and potential significance of Disturbance Sensitive Zone (DSZ) in time-frequency domain. The experimental study of Barely Visible Impact Damage (BVID) on Carbon Fiber Reinforced Polymer (CFRP) is carried out. The decorrelation coefficients of time, frequency, and time-frequency domains and DSZ are calculated and compared. It has been observed that the sensitivity of the scattered wave disturbance caused by impact damage is non-uniformly distributed in both the time and frequency domains. This is evident from the non-uniform distribution of the decorrelation coefficient in time-domain and frequency-domain decorrelation calculations. Further, the decorrelation calculation in the time-frequency domain can show the distribution of the sensitivity of the scattered wave disturbance in the time domain and frequency domain. The decorrelation coefficients in time, frequency, and time-frequency domains increase monotonically with the number of impacts. In addition, in the time-frequency domain decorrelation calculation results, stable and repetitive DSZ are observed, which means that the specific frequency component of the scattered wave is extremely sensitive to the damage evolution of the impact region at a specific time. Finally, the DSZ obtained from the first 15 impacts is used to improve the decorrelation calculation in the 16-th to 20-th impact. The results show that the increment rate of the improved decorrelation coefficient is 10.22%. This study reveals that the diffusion ultrasonic decorrelation calculation improved by DSZ makes it feasible to evaluate early-stage damage caused by BVID.
Regional Pulmonary Ventilation Assessment Method and System Based on Impedance Sensing Information from the Pentapulmonary Lobes
Authors: Zhang Yapeng , Song Chengxin , He Wei , Zhang Qian , Zhao Pengcheng , Wang Jingang
Published: May 2024

Regional lung ventilation assessment is a critical tool for the early detection of lung diseases and postoperative evaluation. Biosensor-based impedance measurements, known for their non-invasive nature, among other benefits, have garnered significant attention compared to traditional detection methods that utilize pressure sensors. However, solely utilizing overall thoracic impedance fails to accurately capture changes in regional lung air volume. This study introduces an assessment method for lung ventilation that utilizes impedance data from the five lobes, develops a nonlinear model correlating regional impedance with lung air volume, and formulates an approach to identify regional ventilation obstructions based on impedance variations in affected areas. The electrode configuration for the five lung lobes was established through numerical simulations, revealing a power–function nonlinear relationship between regional impedance and air volume changes. An analysis of 389 pulmonary function tests refined the equations for calculating pulmonary function parameters, taking into account individual differences. Validation tests on 30 cases indicated maximum relative errors of 0.82% for FVC and 0.98% for FEV1, all within the 95% confidence intervals. The index for assessing regional ventilation impairment was corroborated by CT scans in 50 critical care cases, with 10 validation trials showing agreement with CT lesion localization results.
Characterization and Quantitation of Anthocyanins of the Pigmented Tea Cultivar TRI 2043 (Camellia sinensis L.) from Sri Lanka
Authors: Hopfstock Philipp , Punyasiri Pitumpe Appuhamilage Nimal , Kiene Mats , Kottawa-Arachchi Jeevan Dananjava , Gök Recep , Winterhalter Peter
Published: May 2024

Tea leaves are rich in diverse bioactive compounds. The tea accession TRI 2043 is unique due to its pigmented leaves caused by anthocyanins, clonal origin, resistance to blister blight disease, and high pubescence density. Because of its peculiarity, TRI 2043 is used to produce high-quality silver tip tea, a premium type of tea that commands high prices. This study was carried out to clarify and elucidate the types of anthocyanins in this particular accession. Four different anthocyanin species were identified and quantitated as cyanidin-3-O-β-d-galactoside and delphinidin-3-O-β-d-galactoside equivalents for leaf blades and stems of the cultivar TRI 2043. The characterization was performed by comparison with commercially available reference substances and further confirmed using ion mobility high-resolution time-of-flight-mass spectrometry (IMS-HRTOF-MS). Quantitation was carried out using ultra-high-performance liquid chromatography ultraviolet–visible detection (UHPLC-UV-vis) with cyanidin-3-O-β-d-glucoside as an internal standard. E- and Z-geometric isomers of 6-p-coumaroyl derivates of delphinidin and cyanidin-3-O-β-d-galactopyranosides were observed, and collision cross section (CCS) values were determined for all four different anthocyanidin species. The content of anthocyanins in leaf blades of cultivar TRI 2043 was 856.32 ± 41.56 µg/g dry weight, with cyanidin being the more abundant anthocyanin (69.8%). Conversely, the stem material contained an anthocyanin amount of 459.5 ± 44.7 µg/g dry weight, with a higher content of delphinidin (69.6%). In summary, an enrichment strategy using analytical membrane chromatography was established to fully elucidate and quantify the anthocyanin profile of plant samples such as the special tea variety TRI 2043.
Density and Composition of Cohabiting Bacteria in Chlorella vulgaris CCAP 211/21A Is Influenced by Changes in Nutrient Supply
Authors: Almalki Wasayf J. , Nwokeoji Alison O. , Vaidyanathan Seetharaman
Published: May 2024

Microalgae have considerable potential as a renewable feedstock for biochemical and bioethanol production that can be employed in processes associated with carbon capture. Large-scale microalgae cultivations are often non-axenic and are often cohabited by bacteria. A better understanding of the influence of cohabiting bacteria on microalgae productivity is required to develop sustainable synthetic co-culture processes at scale. Nutrient limitation is a frequently employed strategy in algal cultivations to accumulate energy reserves, such as lipids and carbohydrates. Here, a non-axenic culture of an estuarine green microalga, Chlorella vulgaris CCAP 211/21A, was studied under nutrient replete and deplete conditions to assess how changes in nutrient supply influenced the cohabiting bacterial population and its association with intracellular carbohydrate accumulations in the alga. Nutrient limitation resulted in a maximum carbohydrate yield of 47%, which was 74% higher than that in nutrient replete conditions. However, the latter condition elicited a 2-fold higher carbohydrate productivity. Three cohabiting bacterial isolates were cultivable from the three culture conditions tested. These isolates were identified using the 16S rRNA gene sequence to belong to Halomonas sp. and Muricauda sp. The composition of the bacterial population varied significantly between the growth conditions and time points. In all cases and at all time points, the dominant species was Halomonas isolates. Nutrient depletion resulted in an apparent loss of Muricauda sp. This finding demonstrates that nutrient supply can be used to control cohabiting bacterial populations in algal cultures, which will enable the development of synthetic co-culture strategies for improving algae productivity.
Influence of Magnetic Field Strength on Intravoxel Incoherent Motion Parameters in Diffusion MRI of the Calf
Authors: Bäuchle Tamara Alice , Stuprich Christoph Martin , Loh Martin , Nagel Armin Michael , Uder Michael , Laun Frederik Bernd
Published: May 2024

Background: The purpose of this study was to investigate the dependence of Intravoxel Incoherent Motion (IVIM) parameters measured in the human calf on B0. Methods: Diffusion-weighted image data of eight healthy volunteers were acquired using five b-values (0–600 s/mm2) at rest and after muscle activation at 0.55 and 7 T. The musculus gastrocnemius mediale (GM, activated) was assessed. The perfusion fraction f and diffusion coefficient D were determined using segmented fits. The dependence on field strength was assessed using Student’s t-test for paired samples and the Wilcoxon signed-rank test. A biophysical model built on the three non-exchanging compartments of muscle, venous blood, and arterial blood was used to interpret the data using literature relaxation times. Results: The measured perfusion fraction of the GM was significantly lower at 7 T, both for the baseline measurement and after muscle activation. For 0.55 and 7 T, the mean f values were 7.59% and 3.63% at rest, and 14.03% and 6.92% after activation, respectively. The biophysical model estimations for the mean proton-density-weighted perfusion fraction were 3.37% and 6.50% for the non-activated and activated states, respectively. Conclusions: B0 may have a significant effect on the measured IVIM parameters. The blood relaxation times suggest that 7 T IVIM may be arterial-weighted whereas 0.55 T IVIM may exhibit an approximately equal weighting of arterial and venous blood.
Inhibitory Impact of Prenatal Exposure to Nano-Polystyrene Particles on the MAP2K6/p38 MAPK Axis Inducing Embryonic Developmental Abnormalities in Mice
Authors: Lv Junyi , He Qing , Yan Zixiang , Xie Yuan , Wu Yao , Li Anqi , Zhang Yuqing , Li Jing , Huang Zhenyao
Published: May 2024

Nanoplastics, created by the fragmentation of larger plastic debris, are a serious pollutant posing substantial environmental and health risks. Here, we developed a polystyrene nanoparticle (PS-NP) exposure model during mice pregnancy to explore their effects on embryonic development. We found that exposure to 30 nm PS-NPs during pregnancy resulted in reduced mice placental weight and abnormal embryonic development. Subsequently, our transcriptomic dissection unveiled differential expression in 102 genes under PS-NP exposure and the p38 MAPK pathway emerged as being significantly altered in KEGG pathway mapping. Our findings also included a reduction in the thickness of the trophoblastic layer in the placenta, diminished cell invasion capabilities, and an over-abundance of immature red cells in the blood vessels of the mice. In addition, we validated our findings through the human trophoblastic cell line, HTR-8/SVneo (HTR). PS-NPs induced a drop in the vitality and migration capacities of HTR cells and suppressed the p38 MAPK signaling pathway. This research highlights the embryotoxic effects of nanoplastics on mice, while the verification results from the HTR cells suggest that there could also be certain impacts on the human trophoblast layer, indicating a need for further exploration in this area.
People’s experiences of their involvement in nursing care: a systematic review protocol
Authors: Santos Diana , Santos Eduardo , Amaral António Fernando
Published: May 2024

Background

People's involvement and participation in their own care are the essential basis of nursing care. This phenomenon can be characterised as an approach based on the integration of the person’s values, beliefs, and preferences during nursing care. This process contributes to improve quality of care, improve satisfaction levels, and result in a better experience for people receiving care. To promote the person’s participation in nursing care, it is necessary to better understand their experiences about this topic.

Aim

To synthesise the available evidence on people’s experiences of their involvement and participation in nursing care in a hospital setting.

Design & setting

A systematic review that will be conducted according to the JBI methodology for systematic reviews of qualitative evidence.

Method

The study selection, critical appraisal, and data extraction will be conducted by two independent reviewers. This review will consider studies with a qualitative approach, published and unpublished, in Portuguese, English, or Spanish, with no temporal limit, which include adults, aged 18 years or older, who have experienced an admission to a hospital, that explored people’s experiences of their involvement and participation in nursing care in hospital ward settings. Findings will be presented using a meta-aggregation approach and narrative format, and the final synthesised findings will be graded according to the ConQual approach.

Conclusion

It is expected that this qualitative synthesis will inform people, health professionals, and policymakers, allowing them to develop recommendations to promote the person’s participation in nursing care.
Implementasi Framework TOGAF ADM Bagian Kemahasiswaan Universitas Dinamika Bangsa Jambi
Authors: Sika Xaverius , Astri Lola Yorita , Kisbianty Desi , Setiawan Roby , Irawan
Published: May 2024

Tidak diragukan lagi, pemanfaatan teknologi informasi yang memadai adalah bagian penting dari sistem pendidikan. Hal ini disebabkan karena sistem informasi dapat mempercepat pekerjaan, membuat pekerjaan lebih efektif, dan membuat pemangku kepentingan dapat membuat keputusan dengan cepat. Namun, pada kenyataannya, tidak banyak perguruan tinggi yang menggunakan sistem informasi dalam semua aspeknya. Ini juga berlaku untuk Universitas Dinamika Bangsa Jambi. Walaupun ada beberapa sistem informasi, bagian kemahasiswaan tidak memiliki yang khusus untuk menunjang proses bisnis yang sedang berlangsung. Akibatnya, penelitian ini menghasilkan cetak biru yang terdiri dari enam aplikasi yang dapat digunakan sebagai pedoman untuk pengembangan sistem informasi dengan berpedoman pada kerangka kerja TOGAF ADM, khususnya pada fase preliminary, fase visi arsitektur, fase arsitektur bisnis, fase arsitektur sistem informasi, dan fase teknologi.
Merkmale (nicht-)traditioneller Lehramtsstudierender
Authors: Groß Ophoff Jana , Weber Christoph , Frey Anne , Gamsjäger Manuela
Published: May 2024

Angesichts des akuten Lehrkräftemangels ergreifen in Österreich vermehrt auch nicht-traditionelle Studierende wie Quereinsteiger:innen den Lehrer*innenberuf. Im Zuge der Erforschung neu eingeführter Studiengänge wird deutlich, dass sich diese nicht-traditionellen Studierenden von traditionellen Studierenden unterscheiden, und zwar nicht nur in demografischen Merkmalen, sondern anfänglich auch in ihren professionsbezogenen Überzeugungen. Aus den im Beitrag vorgestellten Unterschieden lassen sich Implikationen für die Ausbildung nicht-traditioneller Studierender ableiten.