bluuebunny/crossref_metadata_2025 huggingface dataset:
In vitro efficacy of Er:YAG laser-activated irrigation versus passive ultrasonic irrigation and sonic-powered irrigation for treating multispecies biofilms in artificial grooves and dentinal tubules: an SEM and CLSM study
Authors: Bao Pingping , Liu He , Yang Lan , Zhang Lulu , Yang Liwei , Xiao Nannan , Shen Jing , Deng Jiayin , Shen Ya
Published: February 2024

Background

Multispecies biofilms located in the anatomical intricacies of the root canal system remain the greatest challenge in root canal disinfection. The efficacy of Er:YAG laser-activated irrigation techniques for treating multispecies biofilms in these hard-to-reach areas has not been proved. The objective of this laboratory study was to evaluate the effectiveness of two Er:YAG laser-activated irrigation techniques, namely, photon-induced photoacoustic streaming (PIPS) and shock wave-enhanced emission photoacoustic streaming (SWEEPS), in treating multispecies biofilms within apical artificial grooves and dentinal tubules, in comparison with conventional needle irrigation (CNI), passive ultrasonic irrigation (PUI), and sonic-powered irrigation (EDDY). Two types of multispecies root canal biofilm models were established in combination with two assessment methods using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) with the aim to obtain more meaningful results.

Methods

Ninety extracted human single-rooted premolars were chosen for two multispecies biofilm models. Each tooth was longitudinally split into two halves. In the first model, a deep narrow groove was created in the apical segment of the canal wall. After cultivating a mixed bacterial biofilm for 4 weeks, the split halves were reassembled and subjected to five irrigation techniques: CNI, PUI, EDD, PIPS, and SWEEPS. The residual biofilms inside and outside the groove in Model 1 were analyzed using SEM. For Model 2, the specimens were split longitudinally once more to evaluate the percentage of killed bacteria in the dentinal tubules across different canal sections (apical, middle, and coronal thirds) using CLSM. One-way analysis of variance and post hoc multiple comparisons were used to assess the antibiofilm efficacy of the 5 irrigation techniques.

Results

Robust biofilm growth was observed in all negative controls after 4 weeks. In Model 1, within each group, significantly fewer bacteria remained outside the groove than inside the groove (P < 0.05). SWEEPS, PIPS and EDDY had significantly greater biofilm removal efficacy than CNI and PUI, both from the outside and inside the groove (P < 0.05). Although SWEEPS was more effective than both PIPS and EDDY at removing biofilms inside the groove (P < 0.05), there were no significant differences among these methods outside the groove (P > 0.05). In Model 2, SWEEPS and EDDY exhibited superior bacterial killing efficacy within the dentinal tubules, followed by PIPS, PUI, and CNI (P < 0.05).

Conclusion

Er:YAG laser-activated irrigation techniques, along with EDDY, demonstrated significant antibiofilm efficacy in apical artificial grooves and dentinal tubules, areas that are typically challenging to access.
The therapeutically actionable long non-coding RNA ‘T-RECS’ is essential to cancer cells’ survival in NRAS/MAPK-driven melanoma
Authors: Feichtenschlager Valentin , Chen Linan , Zheng Yixuan James , Ho Wilson , Sanlorenzo Martina , Vujic Igor , Fewings Eleanor , Lee Albert , Chen Christopher , Callanan Ciara , Lin Kevin , Qu Tiange , Hohlova Dasha , Vujic Marin , Hwang Yeonjoo , Lai Kevin , Chen Stephanie , Nguyen Thuan , Muñoz Denise P , Kohwi Yoshinori , Posch Christian , Daud Adil , Rappersberger Klemens , Kohwi-Shigematsu Terumi , Coppé Jean-Philippe , Ortiz-Urda Susana
Published: February 2024

Finding effective therapeutic targets to treat NRAS-mutated melanoma remains a challenge. Long non-coding RNAs (lncRNAs) recently emerged as essential regulators of tumorigenesis. Using a discovery approach combining experimental models and unbiased computational analysis complemented by validation in patient biospecimens, we identified a nuclear-enriched lncRNA (AC004540.4) that is upregulated in NRAS/MAPK-dependent melanoma, and that we named T-RECS. Considering potential innovative treatment strategies, we designed antisense oligonucleotides (ASOs) to target T-RECS. T-RECS ASOs reduced the growth of melanoma cells and induced apoptotic cell death, while having minimal impact on normal primary melanocytes. Mechanistically, treatment with T-RECS ASOs downregulated the activity of pro-survival kinases and reduced the protein stability of hnRNPA2/B1, a pro-oncogenic regulator of MAPK signaling. Using patient- and cell line- derived tumor xenograft mouse models, we demonstrated that systemic treatment with T-RECS ASOs significantly suppressed the growth of melanoma tumors, with no noticeable toxicity. ASO-mediated T-RECS inhibition represents a promising RNA-targeting approach to improve the outcome of MAPK pathway-activated melanoma.
Sexual dysfunction in patients with diabetes: association between remnant cholesterol and erectile dysfunction
Authors: Huang Ke , Yin Shan , Xiao Yunfei , Wang Jiahao , Cui Jianwei , Wang Jia , Bai Yunjin
Published: February 2024

Background

Erectile dysfunction (ED) is closely associated with dyslipidemia; however, it is yet unknown how ED and remnant cholesterol (RC) are related. As such, this research sought to explore the correlation between RC and ED among individuals with diagnosed with diabetes.

Methods

This cross-sectional study used information from 215 males from National Health and Nutrition Examination Survey (NHANES) from 2001 to 2004. RC was calculated as follows: the values of high-density lipoprotein cholesterol (HDL-c) and low-density lipoprotein cholesterol (LDL-c) were subtracted from the total cholesterol (TC) value, while ED diagnoses were based on self-reports. Weighted logistic regression analyses using both univariate and multivariate approaches were conducted to assess the correlation between RC and ED.

Results

After comprehensive adjustment, multivariable logistic regression models revealed a strong correlation between RC and ED in subjects with diabetes (with an odds ratio (OR) of 7.49 and a 95% confidence interval (CI) of 1.98–28.37; P = 0.004). On categorizing RC into 3 grades (T1-T3), the OR corresponding to higher RC grade increased. Despite the results not reaching statistical significance upon categorization, a consistent and statistically significant trend (P for trend < 0.05) was observed.

Conclusion

This study indicated a correlation between increased RC levels and a higher prevalence of ED in diabetic males. RC may serve as a promising predictor of ED in individuals with diabetes. However, additional studies are required to confirm these findings.
Ubiquitin-specific protease 14 targets PFKL-mediated glycolysis to promote the proliferation and migration of oral squamous cell carcinoma
Authors: Zhang Xingming , Geng Lou , Tang Yi , Wang Yingying , Zhang Youping , Zhu Chujiao , Lei Hu , Xu Hanzhang , Zhu Qi , Wu Yingli , Gu Wenli
Published: February 2024

Aberrant upregulation of the ubiquitin-specific protease 14 (USP14) has been found in some malignant tumors, including oral squamous cell carcinoma (OSCC). In this study, we further demonstrated that aberrantly overexpressed USP14 was also closely related to adverse clinicopathological features and poor prognosis in patients with OSCC, so we hypothesized that USP14 might act as a tumor-promoting factor during the progression of OSCC. Notably, we originally proved that USP14 is a deubiquitinating enzyme for phosphofructokinase-1 liver type (PFKL), a key rate-limiting enzyme involved in the glycolytic pathway. USP14 interacts with PFKL and enhances its stability through deubiquitination in OSCC cells, which in turn enhances PFKL-mediated glycolytic metabolism and ultimately promote cellular proliferation, migration, and tumorigenesis. In this work, we have also demonstrated for the first time that USP14 is a critical regulator of glycolysis in OSCC and verified a novel mechanism whereby it is involved in tumor metastasis and growth. Collectively, our findings provide novel insights into the tumor-promoting role of USP14 and establish mechanistic foundations for USP14-targeting therapies.
HERVK-mediated regulation of neighboring genes: implications for breast cancer prognosis
Authors: Liang Boying , Yan Tengyue , Wei Huilin , Zhang Die , Li Lanxiang , Liu Zengjing , Li Wen , Zhang Yuluan , Jiang Nili , Meng Qiuxia , Jiang Guiyang , Hu Yanling , Leng Jing
Published: February 2024

Human endogenous retroviruses (HERVs) are the remnants of ancient retroviral infections integrated into the human genome. Although most HERVs are silenced or rendered inactive by various regulatory mechanisms, they retain the potential to influence the nearby genes. We analyzed the regulatory map of 91 HERV-Ks on neighboring genes in human breast cancer and investigated the impact of HERV-Ks on the tumor microenvironment (TME) and prognosis of breast cancer. Nine RNA-seq datasets were obtained from GEO and NCBI SRA. Differentially expressed genes and HERV-Ks were analyzed using DESeq2. Validation of high-risk prognostic candidate genes using TCGA data. These included Overall survival (multivariate Cox regression model), immune infiltration analysis (TIMER), tumor mutation burden (maftools), and drug sensitivity analysis (GSCA). A total of 88 candidate genes related to breast cancer prognosis were screened, of which CD48, SLAMF7, SLAMF1, IGLL1, IGHA1, and LRRC8A were key genes. Functionally, these six key genes were significantly enriched in some immune function-related pathways, which may be associated with poor prognosis for breast cancer (p = 0.00016), and the expression levels of these genes were significantly correlated with the sensitivity of breast cancer treatment-related drugs. Mechanistically, they may influence breast cancer development by modulating the infiltration of various immune cells into the TME. We further experimentally validated these genes to confirm the results obtained from bioinformatics analysis. This study represents the first report on the regulatory potential of HERV-K in the neighboring breast cancer genome. We identified three key HERV-Ks and five neighboring genes that hold promise as novel targets for future interventions and treatments for breast cancer.
Artificial intelligence’s impact on breast cancer pathology: a literature review
Authors: Soliman Amr , Li Zaibo , Parwani Anil V.
Published: February 2024

This review discusses the profound impact of artificial intelligence (AI) on breast cancer (BC) diagnosis and management within the field of pathology. It examines the various applications of AI across diverse aspects of BC pathology, highlighting key findings from multiple studies. Integrating AI into routine pathology practice stands to improve diagnostic accuracy, thereby contributing to reducing avoidable errors. Additionally, AI has excelled in identifying invasive breast tumors and lymph node metastasis through its capacity to process large whole-slide images adeptly. Adaptive sampling techniques and powerful convolutional neural networks mark these achievements. The evaluation of hormonal status, which is imperative for BC treatment choices, has also been enhanced by AI quantitative analysis, aiding interobserver concordance and reliability. Breast cancer grading and mitotic count evaluation also benefit from AI intervention. AI-based frameworks effectively classify breast carcinomas, even for moderately graded cases that traditional methods struggle with. Moreover, AI-assisted mitotic figures quantification surpasses manual counting in precision and sensitivity, fostering improved prognosis. The assessment of tumor-infiltrating lymphocytes in triple-negative breast cancer using AI yields insights into patient survival prognosis. Furthermore, AI-powered predictions of neoadjuvant chemotherapy response demonstrate potential for streamlining treatment strategies. Addressing limitations, such as preanalytical variables, annotation demands, and differentiation challenges, is pivotal for realizing AI’s full potential in BC pathology. Despite the existing hurdles, AI’s multifaceted contributions to BC pathology hold great promise, providing enhanced accuracy, efficiency, and standardization. Continued research and innovation are crucial for overcoming obstacles and fully harnessing AI’s transformative capabilities in breast cancer diagnosis and assessment.

Graphical Abstract
Selective enhancement of fear extinction by inhibiting neuronal adenylyl cyclase 1 (AC1) in aged mice
Authors: Shi Wantong , Chen Qi-Yu , Ma Yujie , Wan Jinjin , Li Xu-Hui , Zhuo Min
Published: February 2024

Adenylyl cyclase 1 (AC1) is a selective subtype of ACs, which is selectively expressed in neurons. The activation of AC1 is activity-dependent, and AC1 plays an important role in cortical excitation that contributes to chronic pain and related emotional disorders. Previous studies have reported that human-used NB001 (hNB001, a selective AC1 inhibitor) produced analgesic effects in different animal models of chronic pain. However, the potential effects of hNB001 on learning and memory have been less investigated. In the present study, we found that hNB001 affected neither the induction nor the expression of trace fear, but selectively enhanced the relearning ability during the extinction in aged mice. By contrast, the same application of hNB001 did not affect recent, remote auditory fear memory, or remote fear extinction in either adult or aged mice. Furthermore, a single or consecutive 30-day oral administration of hNB001 did not affect acute nociceptive response, motor function, or anxiety-like behavior in either adult or aged mice. Our results are consistent with previous findings that inhibition of AC1 did not affect general sensory, emotional, and motor functions in adult mice, and provide strong evidence that inhibiting the activity of AC1 may be beneficial for certain forms of learning and memory in aged mice.
Total laparoscopic partial hepatectomy versus open partial hepatectomy for primary left-sided hepatolithiasis: study protocol for a randomized controlled trial
Authors: Pan Shu-bo , Wu Chun-li , Zhou Da-chen , Xiong Qi-ru , Geng Xiao-ping , Hou Hui
Published: February 2024

Background

The advantages of laparoscopic left-sided hepatectomy (LLH) for treating hepatolithiasis in terms of the time to postoperative length of hospital stay (LOS), morbidity, long-term abdominal wall hernias, hospital costs, residual stone rate, and recurrence of calculus have not been confirmed by a randomized controlled trial. The aim of this trial is to compare the safety and effectiveness of LLH with open left-sided hepatectomy (OLH) for the treatment of hepatolithiasis.

Methods

Patients with hepatolithiasis eligible for left-sided hepatectomy will be recruited. The experimental design will produce two randomized arms (laparoscopic and open hepatectomy) at a 1:1 ratio and a prospective registry. All patients will undergo surgery in the setting of an enhanced recovery after surgery (ERAS) programme. The prospective registry will be based on patients who cannot be randomized because of the explicit treatment preference of the patient or surgeon or because of ineligibility (not meeting the inclusion and exclusion criteria) for randomization in this trial. The primary outcome is the LOS. The secondary outcomes are percentage readmission, morbidity, mortality, hospital costs, long-term incidence of incisional hernias, residual stone rate, and recurrence of calculus. It will be assumed that, in patients undergoing LLH, the length of hospital stay will be reduced by 1 day. A sample size of 86 patients in each randomization arm has been calculated as sufficient to detect a 1-day reduction in LOS [90% power and α = 0.05 (two-tailed)]. The trial is a randomized controlled trial that will provide evidence for the merits of laparoscopic surgery in patients undergoing liver resection within an ERAS programme.

Conclusions

Although the outcomes of LLH have been proven to be comparable to those of OLH in retrospective studies, the use of LLH remains restricted, partly due to the lack of short- and long-term informative RCTs pertaining to patients with hepatolithiasis in ERAS programmes. To evaluate the surgical and long-term outcomes of LLH, we will perform a prospective RCT to compare LLH with OLH for hepatolithiasis within an ERAS programme.

Trial registration

ClinicalTrials.gov NCT03958825. Registered on 21 May 2019.
Comparison of 2D 4K vs. 3D HD laparoscopic imaging systems in bariatric surgery: study protocol for a randomized controlled prospective trial
Authors: Zwimpfer Tibor A. , Stiegeler Nadja , Müller Philip C. , Schötzau Andreas , Fellmann-Fischer Bernhard , Heinzelmann-Schwarz Viola , Peterli Ralph , Kraljević Marko
Published: February 2024

Background

Vision is an important and defining element of laparoscopy and significantly affects the outcome of surgery in terms of time, error, and precision. Several new imaging systems have become available for laparoscopic surgery, including three-dimensional (3D) high-definition (HD) and two-dimensional (2D) ultra-high-resolution (4K) monitors. 3D HD systems offer a number of potential benefits to surgeons and patients over traditional 2D systems, including reduced operating time, blood loss, and hospital stay. However, the performance of 3D systems against the new, ultra-high definition 4K systems is barely known and highly controversial. There is a paucity of studies comparing them in clinical settings. The aim of this study is to compare 2D 4K and 3D HD perspectives in gastric bypass surgery.

Methods

Forty-eight patients with an indication for gastric bypass will be randomized to receive laparoscopic gastric bypass surgery using either 2D 4K or 3D HD systems. The operations will be performed by a well-coordinated team of three senior surgeons. The primary outcome is operative time. Secondary outcomes include intraoperative complications, blood loss, operator workload as assessed by the validated Surg-TLX questionnaire, and postoperative complications according to the Clavien-Dindo classification. An interim analysis is planned after enrollment of 12 participants for each group.

Discussion

This prospective, randomized trial is designed to test the hypothesis that the use of a 3D HD system will result in a significant improvement in operative time compared to a 2D 4K system in bariatric surgery. The objective is to provide clinical evidence for new laparoscopic imaging systems and to evaluate potential benefits.

Trial registration

This trial is registered at clinicaltrials.gov under the identifier NCT05895058. Registered 30 May 2023. BASEC2023-D0014 [Registry ID Swissethics, approved 3 May 2023]. SNCTP000005489 [SNCTP study register, last updated 13 July 2023].
Data release: targeted systematic literature search for tick and tick-borne pathogen distributions in six countries in sub-Saharan Africa from 1901 to 2020
Authors: Lilak Abigail A. , Pecor David B. , Matulis Graham , Potter Alexander M. , Wofford Rachel N. , Kearney Mary F. , Mitchell Stephanie , Jaradat Fatima , Kano Arisa , Zimmerman Dawn M. , Hassell James M. , Kumsa Bersissa , Kamau Maureen , Linton Yvonne-Marie , von Fricken Michael E.
Published: February 2024

Background

Surveillance data documenting tick and tick-borne disease (TBD) prevalence is needed to develop risk assessments and implement control strategies. Despite extensive research in Africa, there is no standardized, comprehensive review.

Methods

Here we tackle this knowledge gap, by producing a comprehensive review of research articles on ticks and TBD between 1901 and 2020 in Chad, Djibouti, Ethiopia, Kenya, Tanzania, and Uganda. Over 8356 English language articles were recovered. Our search strategy included 19 related MeSH terms. Articles were reviewed, and 331 met inclusion criteria. Articles containing mappable data were compiled into a standardized data schema, georeferenced, and uploaded to VectorMap.

Results

Tick and pathogen matrixes were created, providing information on vector distributions and tick–pathogen associations within the six selected African countries.

Conclusions

These results provide a digital, mappable database of current and historical tick and TBD distributions across six countries in Africa, which can inform specific risk modeling, determine surveillance gaps, and guide future surveillance priorities.

Graphical Abstract
Human cercarial dermatitis (HCD) in the UK: an overlooked and under-reported nuisance?
Authors: Kerr Orla , Juhász Alexandra , Jones Sam , Stothard J. Russell
Published: February 2024

Background

Human cercarial dermatitis (HCD) is a clinical disease typically caused by skin-penetrative larvae of avian schistosomes. Its geographical epidemiology is firmly tied with that of infected freshwater intermediate snail hosts. To better understand the current distribution of HCD and its level of nuisance in the UK, we undertook a systematic literature review.

Methods

Following PRIMSA guidelines, PubMed and Scopus databases were searched with keywords “human cercarial dermatitis” OR “swimmer’s itch” AND “United Kingdom”. Articles about imported cases of HCD, or HCD outside the UK, were not formally included.

Results

A total of 30 articles were initially identified. A further two were gained by inspection of all citations. After screening, eight publications were analysed where the location, number of cases and putative avian schistosome species incriminated were tabulated. HCD is mainly found in the south of England, though gaps in evidence and reporting remain across the UK.

Conclusions

Despite its noted recent rise in open water swimmers, published literature on HCD across the UK is sparse; this condition is both overlooked and under-reported. We therefore recommend establishing a national database that raises awareness and encourages self-reporting of this nuisance disease.

Graphical Abstract
Hip adduction and abduction strength profiles in elite and sub-elite female soccer players according to players level and leg limb-dominance
Authors: Jaenada-Carrilero Eloy , Vicente-Mampel Juan , Baraja-Vegas Luis , Thorborg Kristian , Valero-Merlos Eloína , Blanco-Gímenez Paula , Gargallo Pedro , Bautista Iker J.
Published: February 2024

Background

Understanding the hip adduction and abduction strength in female soccer players is crucial for performance enhancement and injury prevention. This study compares the strength profiles in these muscle groups between elite and sub-elite female soccer players and assesses the impact of leg limb-dominance.

Methods

A descriptive-comparative study was employed. Eighty-two female soccer players were evaluated. Isometric hip-adduction and abduction strength were measured using a handheld dynamometer.

Results

Female elite and sub-elite soccer players displayed a mean and standard deviation (SD) on isometric hip-adductor strength for dominant (3.19 Nm/kg ± 0.69 vs. 2.40 Nm/kg ± 0.67) and non-dominant leg (3.32 Nm/kg ± 0.76 versus 2.42 Nm/kg ± 0.70), respectively. For isometric hip-abductor strength in elite and sub-elite players, a mean and SD of dominant (2.86 Nm/kg ± 0.56 vs. 2.07 Nm/kg ± 0.50) and non-dominant (2.80 Nm/kg ± 0.59 vs. 2.04 Nm/kg ± 0.43). In essence, elite players were stronger than sub-elite players on isometric hip-adduction (mean difference [MD] = 0.82 Nm/kg, CI95% = 0.42–1.12) and abduction (MD = 0.83 Nm/kg, CI95% = 0.54- 1.12) both in dominant and non-dominant, leg, whereas no differences existed for hip adduction:abduction ratios between groups and legs.

Conclusions

Elite female athletes exhibited greater strength than sub-elite female players in both hip adduction and abduction, whereas adduction:abduction ratio values did not differ between the two groups or between different legs.
Myoid gonadal stromal tumor of the testis—the novel subtype of testicular gonadal stromal tumors: a case report and review of the literature
Authors: Dieckmann Klaus-Peter , Tharun Lars , Angerer Markus , Harms Alexander , Wülfing Christian
Published: February 2024

Background

Sex cord gonadal stromal tumors compose less than 10% of all testicular neoplasms and consist of a variety of histological subtypes. In 2016, the World Health Organization introduced a novel subtype, the myoid gonadal stromal tumor, that consists of spindle-shaped cells with immunohistologic features of muscle cells. Only few cases have been reported to date. Due to its rarity and owing to its only recent introduction, the current knowledge about myoid gonadal stromal tumor is limited, and particularly, appropriate clinical management is still ill-defined.

Case presentation

A 47-year-old man of Caucasian descent presented with nonspecific scrotal discomfort. A roundish and well demarcated hypoechoic mass of 8.5 mm in diameter was detected in the cranial region of the left testis. Serum tumor marker levels were within normal ranges. Testis-sparing surgery revealed a 9-mm whitish, hard mass with sharp surgical margin. Histologically, the neoplasm consisted of microfibrillar tissue with spindle-shaped cells harboring elongated nuclei. Immunohistochemical work-up disclosed expression of desmin, small muscle actin, and S100 protein giving evidence for the myogenic nature of the neoplastic cells. There was no indication of malignancy, neither histologically nor clinically. Follow-up of 1 year was uneventful.

Conclusion

A literature survey revealed 22 previous cases of myoid gonadal stromal tumor. The median age was 37 years, the median size of the neoplasm was 20 mm, and there was no side-preponderance. Myoid gonadal stromal tumor is not much different from other subtypes of gonadal stromal tumors nor from testicular gem cell tumors regarding age and laterality; however, tumor size is smaller in myoid gonadal stromal tumors than in germ cell tumors. Although rarely performed so far, testis-sparing surgery probably constitutes an appropriate treatment of this neoplasm. Myoid gonadal stromal tumor represents an emerging novel entity of benign testicular new growths that caregivers of patients with testicular tumors should be aware of.
Existence and stability of a q-Caputo fractional jerk differential equation having anti-periodic boundary conditions
Authors: Khalid Khansa Hina , Zada Akbar , Popa Ioan-Lucian , Samei Mohammad Esmael
Published: February 2024

In this work, we analyze a q-fractional jerk problem having anti-periodic boundary conditions. The focus is on investigating whether a unique solution exists and remains stable under specific conditions. To prove the uniqueness of the solution, we employ a Banach fixed point theorem and a mathematical tool for establishing the presence of distinct fixed points. To demonstrate the availability of a solution, we utilize Leray–Schauder’s alternative, a method commonly employed in mathematical analysis. Furthermore, we examine and introduce different kinds of stability concepts for the given problem. In conclusion, we present several examples to illustrate and validate the outcomes of our study.
Artificial light at night (ALAN) pollution alters bat lunar chronobiology: insights from broad-scale long-term acoustic monitoring
Authors: Li Han , Allen Pauline , Boris Saige , Lagrama Samantha , Lyons Jade , Mills Christina , Moussi Pauline , Nichols Casey , Tacosik Carter , Tsaousis McKenzie , Livingston Wilson Nyzaya , Grider John F. , Parker Kevin A. , Kalcounis-Rueppell Matina C.
Published: February 2024

Background

The timing of behavior and habitat use of nocturnal animals can be influenced by the lunar cycle in nature. The prevalence of artificial light at night (ALAN) has been recognized as a source of environmental pollution. The interaction between ALAN and the lunar cycle on bat behavior is important for understanding anthropogenic effects on bats. We utilized a decade (2012–2022) of acoustic monitoring data collected in North Carolina, United States, to investigate the relationship between bat activity, lunar cycle, and light pollution. We examined whether the amount of lunar illumination affected species-specific nightly activity and whether hourly bat activity patterns varied between nights with different moon phases. We further investigated if the relationship between bat activity and the lunar cycle might be altered by light pollution.

Results

We found that seven bat species showed activity variation across nights in relation to the amount of moon illumination when ALAN was absent. In general, bats were less active on full moon nights compared to new moon nights. Light pollution interacted with the bat–lunar relationship in five of the seven species, masking the effect of the lunar cycle. We identified delayed bat activity patterns on nights with a full or waxing moon in seven species, and light pollution altered that pattern in four species. Overall, ALAN was associated with decreased bat activity independent of lunar cycle effects.

Conclusions

Our study demonstrated that at a broad spatial scale, ALAN negatively affected many North American temperate bat species and altered their lunar chronobiology. As light pollution is spreading to historically dark areas and habitats, ALAN might couple with other threats, such as the white-nose syndrome or climate change, to cause cascading damage in the environment that depends on ecosystem services such as pest control provided by bats. We argue that further research and conservation actions are needed to mitigate the impact of light pollution.
Multi-scale habitat influences sprainting and group size of a freshwater-obligate smooth-coated otter (Lutrogale perspicillata) in Tungabhadra Otter Conservation Reserve, India
Authors: Moun Ankit , Kumar P. Ramesh , Priya M. Malathi , Ramesh T. , Kalle Riddhika
Published: February 2024

Background

The impact of changing land-use patterns and associated anthropogenic threats on scale-dependent habitat use of semi-aquatic mustelids in scent-marking and social behaviour can provide important insights into the habitat ecology of smooth-coated otters (Lutrogale perspicillata).

Methods

We sampled 180 stream segments (100–400 m) as spatial replicates of 60 1-km2 sites to record indirect evidence (i.e. spraints and mass latrine sites) and group sizes of smooth-coated otters along the Tungabhadra Otter Conservation Reserve (TOCR) during the dry season. To quantify habitat, we recorded stream characteristics, riparian vegetation, and anthropogenic disturbances at the local scale, and hydro-environmental characteristics and land uses at the landscape scale. Using Markovian-chain detection and occupancy models, we assessed the multi-scale habitat use of otters in their selection of suitable areas for scent-marking based on repeated presence-absence data on spraint/latrine locations along the TOCR. We further used linear regression techniques to explore relationships between the number of individuals in smooth-coated otter group and hydro-environmental characteristics, spraint/latrine encounter rate, anthropogenic pressure, land cover, topography, and vegetation.

Results

At the local scale, the probability of spraint deposition and group size decreased with anthropogenic disturbance while the probability of spraint detections decreased with grass cover. At the landscape scale, the probability of otter site use for spraint deposition and group sizes increased in southeast flowing streams. Spraint deposition increased with the proportion of sugarcane fields, whereas in contrast, group size decreased with proportion of sugarcane fields.

Conclusions

Our findings highlight the first empirical evidence on multi-scale habitat use of a southern Indian population of smooth-coated otters in an inland freshwater ecosystem surrounded by the scrub jungle–agriculture matrix. We suggest that habitat models built from analytical approaches that account for correlated detections can avoid biased predictions when estimating occupancy and detection probability of semi-aquatic or riparian mammal communities with linear distributions. Our findings indicate that human activity can impose constraints on the choice of sites used for spraint deposition and preliminary patterns in otter groups. The study provides some crucial evidence on the need to maintain areas with minimal human interference for sustainability of freshwater reserves.
Comparison of two 3D scanning software to identify facial features: a prospective instrument to predict difficult airway
Authors: Rocuts Alexander , Avella-Molano Bibiana , Behr Amanda , Lakhani Farhan , Bolds Bryant , Riveros-Amado Matias , Riveros-Perez Efrain
Published: February 2024

Background

Clinical airway assessment has limited predictive ability to anticipate difficult airway. Three-dimensional (3D) technologies have emerged in medicine as valuable tools in different settings including innovation and surgical planning. Three-dimensional facial scanning could add value to clinical measurements and two-dimensional models to assess the airway. However, commonly used high-fidelity scans are expensive. This study aims to compare the accuracy of the measurements made by the Scandy Pro app as a cost-effective alternative to high-fidelity scans made by the Artec Space Spider. We also aim to evaluate the interobserver variability for the measurements performed with Scandy Pro.

Materials and methods

We conducted a cross-sectional, comparison study on 10 healthy volunteers. Four observers measured 720 distances and 400 using both Scandy Pro and Artec Space Spider facial scans. Wilcoxon test was used for group–group comparison.

Results

Comparison of both instruments showed no difference in angle or distance measurements. The percentage error (measurement difference between the two devices) exhibited by one of the observers was significantly different compared with the other three observers; however, the magnitude of this individual deviation did not affect the overall percentage error. The overall error for Scandy Pro was 5.5% (3.9% and 6.7% for angles and distances, respectively).

Conclusion

Three-dimensional facial scanning with Scandy Pro is an accurate tool that can be a cost-effective alternative to high-fidelity scans produced by the Artec Space Spider.
Polyvascular atherosclerosis and renal dysfunction increase the odds of cognitive impairment in vascular disease: findings of the LipidCardio study
Authors: König Maximilian , Palmer Katie , Malsch Carolin , Steinhagen-Thiessen Elisabeth , Demuth Ilja
Published: February 2024

Introduction

Growing evidence suggests a causal role for atherosclerotic vascular disease in cognitive impairment and dementia. Atherosclerosis may present as monovascular disease (monoVD) or as widespread polyvascular atherosclerotic disease (polyVD). Evidence on the relationship between monoVD or polyVD and cognitive impairment is limited.

Methods

We conducted a cross-sectional analysis of baseline data from the LipidCardio Study. The main outcome measure was the presence of cognitive impairment, defined as a Mini-Mental State Examination (MMSE) score < 26.

Results

The mean age was 71.5 years, 30.3% were female, 17.3% had no evidence of large-vessel atherosclerosis, 71.1% had monoVD, and 11.7% had polyVD, defined as the presence of atherosclerosis in ≥ 2 vascular territories (coronary, cerebral, aortic, or lower extremity). A total of 21.6% had cognitive impairment according to the prespecified cutoff (MMSE < 26). Overall, the odds of cognitive impairment increased for each additional vascular territory affected by atherosclerosis [adjusted odds ratio 1.76, 95% confidence interval (CI) 1.21–2.57, p = 0.003]. Furthermore, there was evidence for an interaction between vascular disease and chronic kidney disease (CKD). The odds of cognitive impairment were not greater in the monoVD subgroup compared to those without any atherosclerosis, if CKD was absent (OR 0.98, 95% CI 0.48–2.10; p = 0.095), while the odds ratio (OR) of cognitive impairment with polyVD compared to no atherosclerosis was 2.71 (95% CI 1.10–6.92; p = 0.031). In contrast, in patients with CKD, both monoVD and polyVD were associated with significantly higher odds of cognitive impairment than no atherosclerosis.

Conclusions

PolyVD is associated with increased odds of cognitive impairment. MonoVD is associated with cognitive impairment only in the presence of CKD.
Correlations between multimodal neuroimaging and peripheral inflammation in different subtypes and mood states of bipolar disorder: a systematic review
Authors: Long Jing-Yi , Li Bo , Ding Pei , Mei Hao , Li Yi
Published: February 2024

Background

Systemic inflammation-immune dysregulation and brain abnormalities are believed to contribute to the pathogenesis of bipolar disorder (BD). However, the connections between peripheral inflammation and the brain, especially the interactions between different BD subtypes and episodes, remain to be elucidated. Therefore, we conducted the present study to provide a comprehensive understanding of the complex association between peripheral inflammation and neuroimaging findings in patients with bipolar spectrum disorders.

Methods

This systematic review was registered in the International Prospective Register of Systematic Reviews (PROSPERO) database (CRD42023447044) and conducted according to the Population, Intervention, Comparison, Outcomes, and Study Design (PICOS) framework. Online literature databases (PubMed, Web of Science, Scopus, EMBASE, MEDLINE, PsycINFO, and the Cochrane Library) were searched for studies that simultaneously investigated both peripheral inflammation-related factors and magnetic resonance neurography of BD patients up to July 01, 2023. Then, we analysed the correlations between peripheral inflammation and neuroimaging, as well as the variation trends and the shared and specific patterns of these correlations according to different clinical dimensions.

Results

In total, 34 publications ultimately met the inclusion criteria for this systematic review, with 2993 subjects included. Among all patterns of interaction between peripheral inflammation and neuroimaging, the most common pattern was a positive relationship between elevated inflammation levels and decreased neuroimaging measurements. The brain regions most susceptible to inflammatory activation were the anterior cingulate cortex, amygdala, prefrontal cortex, striatum, hippocampus, orbitofrontal cortex, parahippocampal gyrus, postcentral gyrus, and posterior cingulate cortex.

Limitations

The small sample size, insufficiently explicit categorization of BD subtypes and episodes, and heterogeneity of the research methods limited further implementation of quantitative data synthesis.

Conclusions

Disturbed interactions between peripheral inflammation and the brain play a critical role in BD, and these interactions exhibit certain commonalities and differences across various clinical dimensions of BD. Our study further confirmed that the fronto-limbic-striatal system may be the central neural substrate in BD patients.
ADHD learners as victims or survivors in L2 learning contexts: a case of application of dynamic assessment to selective attention and reading comprehension ability
Authors: Salehi Hadiseh , Khoii Roya , Rashtchi Mojgan , Arjmandnia Ali Akbar
Published: February 2024

With the growing concern for the issues and problems associated with static testing, dynamic assessment (DA) has been recently receiving increased attention as an alternative in educational settings. The present single case study attempted to investigate the potential of interactionist dynamic assessment in developing an Attention Deficit Hyperactivity Disorder (ADHD) L2 learner’s reading competence and selective attention. A 13-year-old female EFL learner with the combined type of ADHD voluntarily participated in this study. During the DA sessions, the learner read some short passages and answered the questions through mediations emerging from the interactions between the mediator and the learner. The qualitative analysis of DA protocols led to the development of an inventory of different forms of implicit and explicit mediations. Moreover, the quantitative analysis of the data showed that DA contributed to the learner’s reading comprehension and some congruent parameters of selective attention. The results of the present study call for teachers’, policymakers’, and material developers’ increasing attention to the mediations required to assist ADHD L2 learner’s independence from the mediator, which demonstrates self-regulation.
Barriers to Timely Seeking of Breast Cancer Care Among Palestinian Women: A Cross-Sectional Study
Authors: Elshami Mohamedraed , Qawasmi Malak Ayman , Ghithan Roba Jamal , Al-Slaibi Ibrahim , Alser Mohammed , Shurrab Nouran Ramzi , Ismail Islam Osama , Mahfouz Ibtisam Ismail , AbdulQader Fannon Aseel , Hawa Mona Radi , Giacaman Narmeen , Ahmaro Manar , Okshiya Heba Mahmoud , Zaatreh Rula Khader , AbuKhalil Wafa Aqel , Usrof Faten Darwish , Melhim Noor Khairi , Madbouh Ruba Jamal , Abu Hziema Hala Jamal , Abed-Allateef Lahlooh Raghad , Ubaiat Sara Nawaf , Jaffal Nour Ali , Alawna Reem Khaled , Abed Salsabeel Naeem , Abuzahra Bessan Nimer , Abu Kwaik Aya Jawad , Dodin Mays Hafez , Taha Raghad Othman , Alashqar Dina Mohammed , Mobarak Roaa Abd-alfattah , Smerat Tasneem , Albarqi Shurouq I. , Abu-El-Noor Nasser , Bottcher Bettina
Published: February 2024

PURPOSE

Examining the association of breast cancer (BC) symptom awareness with time to help seeking and exploring barriers to timely presentation may enhance the effectiveness of BC awareness campaigns and early detection efforts. This study aimed to assess the anticipated time for seeking medical advice when experiencing a potential BC symptom among women in Palestine and to identify their barriers to early presentation.

MATERIALS AND METHODS

A convenience sampling method was used to recruit adult women from hospitals, primary health care facilities, and public areas across 11 governorates in Palestine. A translated-into-Arabic version of the validated BC Awareness Measure was used. The questionnaire consisted of three sections: sociodemographic information, recognition of 13 BC symptoms and reporting time for seeking medical advice, and barriers to early presentation.

RESULTS

A total of 5,257 questionnaires were included. The proportion of participants who would seek medical advice immediately varied on the basis of the nature of BC symptoms. For symptoms related to the breast, the proportion ranged from 25.7% for redness of the breast skin to 53.5% for a lump or thickening in the breast. For symptoms related to the nipple, the proportion ranged from 30.7% for nipple rash to 48.0% for discharge or bleeding from the nipple. Exhibiting good BC symptom awareness was associated with a higher likelihood of seeking medical advice within a week for all BC symptoms. Emotional barriers were the most frequently reported barriers. There was no association between increasing levels of BC awareness and reporting fewer or more barriers.

CONCLUSION

The nature of BC symptoms had an impact on help-seeking behaviors. Participants with good BC symptom awareness were more likely to seek medical advice earlier.
A Test of the Validity of Imputed Legal Immigration Status
Authors: Castillo Marcelo , Hill Alexandra , Hertz Thomas
Published: February 2024

We evaluate the performance of a widely used technique for imputing the legal immigration status of U.S. immigrants in survey data—the logical imputation method. We validate this technique by implementing it in a nationally representative survey of U.S. farmworkers that includes a well-regarded measure of legal status. When using this measure as a benchmark, the imputation algorithm correctly identifies the legal status of 78% of farmworkers. Of all the variables included in the algorithm, we find that Medicaid participation poses the greatest challenge for accuracy. Using the American Community Survey, we show that increased Medicaid enrollments stemming from the implementation of the Affordable Care Act in 2014 led to sizable changes in the share of immigrants imputed as legal over time and across space. We explore the implications of these changes for two previous studies and conclude that including Medicaid criteria in the imputation algorithm can significantly impact research findings. We also provide tools to gauge the sensitivity of results.
The Learning Curve and Clinical Outcomes With 250 Laser Ablations for Brain Tumors: A Pathway to Experience
Authors: Merenzon Martin A. , Bhatia Shovan , Levy Adam , Di Long , Gurses Muhammet Enes , Rivera Cameron , Daggubati Lekhaj , Luther Evan , Shah Ashish H. , Komotar Ricardo J. , Ivan Michael E.
Published: February 2024

BACKGROUND AND OBJECTIVES:

Laser interstitial thermal therapy (LITT) has gained popularity as a minimally invasive technique for treating brain tumors. Despite its proven safety profile, LITT is not yet widely available, and there is a lack of data on the learning curve required to achieve proficiency. This study analyzes a 250-patient cohort of laser-ablated tumors to describe changes in patient selection and clinical outcomes over time and experience, with the aim of providing insight into the learning curve for incorporating LITT into a neuro-oncology program and identifying a cutoff point that distinguishes novice from expert performance.

METHODS:

We retrospectively reviewed 250 patients with brain tumor who underwent LITT between 2013 and 2022. Demographic and clinical data were analyzed. Kaplan Meier curves were used for survival analysis. Operative time was evaluated using exponential curve-fit regression analysis to identify when consistent improvement began.

RESULTS:

The patients were divided into quartiles (Q) based on their date of surgery. Mean tumor volume increased over time (Q1 = 5.7 and Q4 = 11.9 cm3, P = .004), and newly diagnosed lesions were more frequently ablated (P = .0001). Mean operative time (Q1 v Q4 = 322.3 v 204.6 min, P < .0001) and neurosurgical readmission rate (Q1 v Q4 = 7.8% v 0%, P = .03) were reduced over time. The exponential curve-fit analysis showed a sustained decay in operative time after case #74. The extent of ablation (P = .69), the recurrence (P = .11), and the postoperative complication rate (P = .78) did not vary over time.

CONCLUSION:

After treating 74 patients, a downward trend in the operative time is observed. Patient selection is broadened as experience increases.
Understanding the Vital Role of Health/Fitness Professionals in Long COVID Recovery
Authors: Sanders Mary E. , Bryant Cedric X. , Vanichkachorn Greg
Published: February 2024

Apply It!

• Understand the challenges that individuals with long COVID face to provide safe and effective steps toward recovery.

• Discover how health/fitness professionals and health care providers can work together to help ensure that individuals with long COVID receive the best possible care and support.

• Recommend activity strategies that lead to positive experiences while nurturing a safe, comfortable, and healthy path to a desirable lifestyle.

• Be compassionate and encourage clients to practice patience to balance hope for normalcy with honesty about personal capabilities.

Editor’s note – With the rapidly changing research surrounding COVID and long COVID, it is important to note that due to production deadlines, the information contained within this article was compiled in October 2023, and may not include the latest information available.
Surgical aspect of patient care with cleidocranial dysplasia - case report
Authors: Szczepkowska Aleksandra , Osica Piotr , Janas-Naze Anna
Published: February 2024

W pracy przedstawiono przypadek pierwszego etapu leczenia ortodontyczno-chirurgicznego 16-letniej pacjentki z dysplazją obojczykowo-czaszkową, przeprowadzonego w znieczuleniu ogólnym.