bluuebunny/crossref_metadata_2025 huggingface dataset:
EFFECT OF SUGAR CANE MORDANT ON THE DYEING OF TEXTILE COLORS USING EXTRACT OF THE SHY PRINCESS PLANT (Mimosa Pudica)
Authors: Rosyadi Farhana Lafaticha , Adriani Adriani
Published: November 2024

This study explores the use of natural color sources from the sensitive plant (Mimosa Pudica) with different sugar cane mordants to reduce pollution caused by synthetic dyes. The research aims to examine the color evenness and the impact of different mordants on cotton material dyeing using sensitive plant extracts and black, red, and yellow sugar cane mordants. The study adopts an experimental approach and collects primary data from 15 panelists. The results show that the use of black, red, and yellow sugar cane mordants leads to very flat color flatness. The evenness of color is also significant, with a data value of 0.011 0.05, suggesting a significant effect on the dyeing process of sensitive plant (Mimosa Pudica) extract using black, red, and yellow sugar cane mordants on cotton material.
ASIATIC LILY FLOWER AS A SOURCE OF PARTY CLOTHING CREATION IDEAS
Authors: Salsabillah Laylisa , Yulistiana Yulistiana
Published: November 2024

The Asiatic lily flower has a unique beauty, its bright colors, large and curved petals are very eye-catching. The purpose of writing this article is to describe the process of making and finished party clothing using the idea of the Asiatic Lily flower as a detail of manipulating fabric. The method used is the double diamond model, which consists of 4 stages, namely the discover stage by searching, finding and collecting information to be used as a source of ideas. The define stage is defining the information that has been obtained at the discover stage, then expressed in the form of a moodboard. The develop stage, namely the design ideas that have been described through a moodboard, are realized in the form of fashion sketches, and are developed, tested, revised and refined. The deliver stage is analyzing and explaining the results of the finished fashion design from the source of the Asiatic lily flower idea. The resulting party attire is as expected. The application of manipulating asiatic lily flower fabric succeeded in beautifying the appearance. Apart from that, the addition of manipulating fabric cording and the asymmetrical arrangement of manipulating fabric flowers gives a unique impression to party clothing.
Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Fanconi Anemia: Results of a Single Center with a Fludarabine-Based Conditioning Regimen
Authors: Mekni Sabrine , Torjemane Lamia , Khayati Malek , Belloumi Dorra , Kanoun Rimmel Yosra , Yaiche Insaf Ben , Ouerghi Rihab , Turki Ines , Ladeb Saloua , Othman Tarek Ben , Abdeljelil Nour Ben
Published: November 2024

Allogeneic hematopoietic stem cell transplantation (Allo-HSCT) remains the only curative treatment for bone marrow failure (BMF) and hematologic complications of Fanconi anemia (FA). The evolution remains affected by the toxicity, the risk of graft failure, and clonal evolution. This study aimed to identify factors affecting outcomes in FA patients after allo-HSCT. We included FA patients who underwent allo-HSCT between January 2006 and December 2021. The conditioning regimen was cyclophosphamide/fludarabine (Cy/Flu) ± rabbit anti-thymocyte globulin (ATG). Bone marrow was the stem cell source from HLA-matched related donors in all transplants. Twenty-three patients, 19 with BMF and 4 with MDS/clonal evolution, were included. The median age was 11 years (5-39 years). Five patients (22%) received serotherapy with the conditioning regimen. Engraftment occurred in all patients without severe regimen-related toxicity. The 100-day cumulative incidence (CI) of grade II-IV acute GvHD (a GVHD) and 5-year CI of chronic GvHD (cGVHD) were 17% and 32%, respectively. The 5-year CI of late secondary graft failure was 10.5%. Two patients developed clonal evolution (AML; n = 2) and one patient developed nephrotic syndrome (n = 1). The 10-year overall survival (OS) and event-free survival (EFS) were 80% and 75%, respectively. There was a trend toward a better EFS in patients aged <10 years compared to patients aged ≥10 years (100% versus 61%; p = 0.06). At the last follow-up, 18 patients were alive, and 4 expired. Causes of death were infections with refractory GVHD (n = 1), graft failure (n = 2), and renal failure (n = 1). Despite the small patient population, we show excellent outcomes, particularly for those transplanted in the first decade.
Assessing the Quality of an Online Democratic Deliberation on COVID-19 Pandemic Triage Protocols for Access to Critical Care in an Extreme Pandemic Context: Mixed Methods Study
Authors: Calderon Ramirez Claudia Lucrecia , Farmer Yanick , Downar James , Frolic Andrea , Opatrny Lucie , Poirier Diane , Bravo Gina , L'Espérance Audrey , Gaucher Nathalie , Payot Antoine , Dahine Joseph , Tanuseputro Peter , Rousseau Louis-Martin , Dumez Vincent , Descôteaux Annie , Dallaire Clara , Laporte Karell , Bouthillier Marie-Eve
Published: November 2024

Background

Online democratic deliberation (ODD) may foster public engagement in new health strategies by providing opportunities for knowledge exchange between experts, policy makers, and the public. It can favor decision-making by generating new points of view and solutions to existing problems. Deliberation experts recommend gathering feedback from participants to optimize future implementation. However, this online modality has not been frequently evaluated.

Objective

This study aims to (1) assess the quality of an ODD held in Quebec and Ontario, Canada, on the topic of COVID-19 triage protocols for access to critical care in an extreme pandemic context and (2) determine its transformative aspect according to the perceptions of participants.

Methods

We conducted a simultaneous ODD in Quebec and Ontario on May 28 and June 4, 2022, with a diversified target audience not working in the health care system. We used a thematic analysis for the transcripts of the deliberation and the written comments of the participants related to the quality of the process. Participants responded to a postdeliberation questionnaire to assess the quality of the ODD and identify changes in their perspectives on COVID-19 pandemic triage protocols after the deliberation exercise. Descriptive statistics were used. An index was calculated to determine equality of participation.

Results

The ODD involved 47 diverse participants from the public (n=20, 43% from Quebec and n=27, 57% from Ontario). Five themes emerged: (1) process appreciation, (2) learning experience, (3) reflecting on the common good, (4) technological aspects, and (5) transformative aspects. A total of 46 participants responded to the questionnaire. Participants considered the quality of the ODD satisfactory in terms of process, information shared, reasoning, and videoconferencing. A total of 4 (80%) of 5 participants reported at least 1 change of perspective on some of the criteria and values discussed. Most participants reported that the online modality was accessible and user-friendly. We found low polarization when calculating equal participation. Improvements identified were measures to replace participants when unable to connect and optimization of time during discussions.

Conclusions

Overall, the participants perceived the quality of ODD as satisfactory. Some participants self-reported a change of opinion after deliberation. The online modality may be an acceptable alternative for democratic deliberation but with some organizational adaptations.
A New Research Model for Artificial Intelligence–Based Well-Being Chatbot Engagement: Survey Study
Authors: Yang Yanrong , Tavares Jorge , Oliveira Tiago
Published: November 2024

Background

Artificial intelligence (AI)–based chatbots have emerged as potential tools to assist individuals in reducing anxiety and supporting well-being.

Objective

This study aimed to identify the factors that impact individuals’ intention to engage and their engagement behavior with AI-based well-being chatbots by using a novel research model to enhance service levels, thereby improving user experience and mental health intervention effectiveness.

Methods

We conducted a web-based questionnaire survey of adult users of well-being chatbots in China via social media. Our survey collected demographic data, as well as a range of measures to assess relevant theoretical factors. Finally, 256 valid responses were obtained. The newly applied model was validated through the partial least squares structural equation modeling approach.

Results

The model explained 62.8% (R2) of the variance in intention to engage and 74% (R2) of the variance in engagement behavior. Affect (β=.201; P=.002), social factors (β=.184; P=.007), and compatibility (β=.149; P=.03) were statistically significant for the intention to engage. Habit (β=.154; P=.01), trust (β=.253; P<.001), and intention to engage (β=.464; P<.001) were statistically significant for engagement behavior.

Conclusions

The new extended model provides a theoretical basis for studying users’ AI-based chatbot engagement behavior. This study highlights practical points for developers of AI-based well-being chatbots. It also highlights the importance of AI-based well-being chatbots to create an emotional connection with the users.
A Novel Web App for Dietary Weight Management: Development, Implementation, and Usability Study (Preprint)
Authors: Oliveira Ashleigh , Wolff John , Alfouzan Nouf , Yu Jin , Yahya Asma , Lammy Kayla , Nakamura Manabu T
Published: November 2024

BACKGROUND

Online weight loss programs have ambiguous efficacy. There is a growing body of evidence that weight loss programs when combined with apps have better outcomes; however, many apps lack an evidence-based approach to dietary changes for weight loss and do not rely on a theoretical framework for behavior change.

OBJECTIVE

This study aimed to describe the development and the preliminary usability and acceptability testing of a web app that uses behavior change techniques (BCTs) to support users of a comprehensive online weight loss program.

METHODS

The weight loss program intervention components were nutrient and weight tracking charts that needed a remotely accessible and online format. The app was designed by nutrition researchers and developers in a collaborative effort. A review of BCTs in weight loss and web apps was performed as well as an assessment of user needs to inform the initial prototype. A preliminary app prototype, version 1.0, was provided to participants of a weight loss trial (N=30) to assess for feasibility of its use. A full app prototype, version 2.0, was feasibility and acceptability tested by trial participants (n=11) with formal feedback by Likert-scale survey and open-ended questions. In the final round of testing, a user group of scientists and developers (n=11) was selected to provide a structured 3-month review through which the group met weekly for collective feedback sessions.

RESULTS

The process resulted in a fully developed web app, MealPlot, by the Applied Research Institute, for meal planning and weight tracking that can be used by weight loss users and health professionals to track their patients. MealPlot includes a weight chart, a protein-fiber chart, and a chat feature. In addition, MealPlot has 2 distinct platforms, 1 for weight loss users and 1 for health professionals. Selected BCTs for incorporation into the app were goal setting, feedback, problem-solving, self-monitoring, and social support. Version 1.0 was used successfully to provide a functioning, online weight chart over the course of a 1-year trial. Version 2.0 provided a functional weight chart and meal planning page, but 8 out of 11 participants indicated MealPlot was difficult to use. Version 3.0 was developed based on feedback and strategies provided from user group testing.

CONCLUSIONS

The web app, MealPlot, was developed to improve outcomes and functionality of an online weight loss program by providing a remote method of tracking weight, food intake, and connecting users to health professionals for consistent guidance that is not otherwise available in a traditional in-person health care setting. The final version 3.0 of the web app will be refined based on findings of a review study gathering feedback from health professionals and from actual weight loss users who are part of a clinical weight loss trial.
Large Language Models are good Radiology Report Labelers (Preprint)
Authors: Abdullah Mr , Kim Seong Tae
Published: November 2024

BACKGROUND

Labeling unstructured radiology reports is crucial for creating structured datasets that facilitate downstream tasks, such as training large-scale medical imaging models. Current approaches typically rely on BERT-based methods or manual expert annotations, which have limitations in terms of scalability and performance.

OBJECTIVE

To evaluate the effectiveness of a GPT-based large language model (LLM) in labeling radiology reports, comparing it with two existing methods, CheXbert and CheXpert, on a large chest X-ray dataset (MIMIC-CXR).

METHODS

In this study, we introduce an LLM-based approach fine-tuned on expert-labeled radiology reports. Our model's performance was evaluated on 687 radiologist-labeled chest X-ray reports, comparing F1 scores across 14 thoracic pathologies. The performance of our LLM model was compared with the CheXbert and CheXpert models across positive, negative, and uncertainty extraction tasks. Paired t-tests and Wilcoxon signed-rank tests were performed to evaluate the statistical significance of differences between model performances.

RESULTS

Our GPT-based model achieved an average F1 score of 0.9014 for all certainty levels and 0.8708 for positive/negative certainty levels, outperforming CheXpert (F1 scores of 0.8864 and 0.8525, respectively) and performing comparably to CheXbert (F1 scores of 0.9047 and 0.8733, respectively). Paired t-tests revealed no statistically significant difference between our model and CheXbert (P = 0.3483), but a significant difference between our model and CheXpert (P = 0.0114). The Wilcoxon test also confirmed these results: no significant difference between our model and CheXbert (P = 0.1353) and a significant difference between our model and CheXpert (P = 0.0052).

CONCLUSIONS

The GPT-based LLM model demonstrates competitive performance compared to CheXbert and outperforms CheXpert in radiology report labeling. These findings suggest that LLMs are a promising alternative to traditional BERT-based architectures for this task, offering enhanced context understanding and eliminating the need for extensive feature engineering. Moreover with large context length LLM based models are better suited for this task as compared to the small context length of BERT based models.

CLINICALTRIAL

Not applicable.
Analysis of the Current Situation and Optimization Paths of Teaching Quality in Rural Primary Schools in Ganzi Prefecture under the Background of “Double Reduction”
Authors: Tang Fei
Published: November 2024

To further implement the fundamental task of strengthening moral education and cultivating people, the country issued the “double reduction” policy in 2021. Nowadays, the whole country is reducing the academic burden on students with unprecedented policy intensity. Although the development speed of education in ethnic regions is relatively slow, this policy has also been gradually promoted and implemented in the basic education stage. This article takes “double reduction” as the background and observes the current situation of teaching quality in rural primary schools in Ganzi Prefecture through questionnaires and interviews. It analyzes the objective factors in three aspects: learning quality, teaching quality, and management quality, and proposes optimization paths for teaching quality.
Contemporary Painting and Spiritual Landscape--Re-examination of the History and Theme of Shelf Paintings
Authors: Qin Chuan
Published: November 2024

This paper and the graduation piece continue the course of my research. By investigating the history and development of contemporary painting, of how painting going from representational to abstract, this paper and my graduation piece is aimed to find a new interpretation to integrate the ideas of painting; to achieve the "landscape of spirit" of artist and to interpret the expressive principle of art fully. Finally, combining the landscape of spirit and painting together, highlighting an important factor of creation-"spirit". This paper has taken the analysis from Chinese and Western to be the main content-from representational to abstract; from the West to the East; and from the combination of the East and the West. Of western artists, Picasso, Rothko was chosen to be the representative while the integration of Confucianism, Buddhism and Taoism from the Wei and Jin Dynasty was chosen to represent the East. Whether it is the religion of the West of the philosophy or the East could be found in painting easily, this show that spirit is necessary in painting. Nowadays, the East and the West are closely linked, Chinese artist's self-study and the "landscape of spirit" could be easily seen. How to explain the "landscape of spirit" is also a problem. This paper tries to explain the "landscape of spirit" by combining art history, study of the connection and development of art and technology, and my own art.
DEVELOPMENT OF GABAPENTIN-LOADED SOLID LIPID NANOPARTICLE FOR ALLEVIATING SEIZURE ACTIVITY IN PICROTOXIN AND BICUCULLINE-INDUCED RATS
Authors: AGARWAL POOJA , BAKSHI VASUDHA
Published: November 2024

Objective: The current research aimed to prepare gabapentin-loaded Solid Lipid Nanoparticles (SLN) for alleviating seizure activity in picrotoxin and bicuculline-induced Wistar rats.
Methods: Gabapentin-loaded SLNs were formulated using a Box-Behnken experimental design with three-level three-factor consisting of 17 experimental runs by micro-emulsification. Three independent parameters were considered in this study, namely sodium glyceryl tripalmitate (A), RPM (B), and Poloxamer-188 (C). Particle size, drug release, and Encapsulation Efficiency (EE) as dependent variables. The formulation was evaluated for drug release, EE, Attenuated Total Reflection (ATR), Differential Scanning Calorimetry (DSC), X-ray diffraction (XRD), surface morphology, particle size, zeta potential, in vivo anti-convulsion study.
Results: The data collected during the experiment includes the measurements of EE (Encapsulation Efficiency), drug release at the 12th h, and particle size. It was reported that formulations containing a high concentration of Glyceryl tripalmitate (50%) had a high Encapsulation Efficiency (EE). The in vitro release results indicate that F17 demonstrated a maximum drug concentration of 99.99% within a 12 h. The optimization process was conducted using mathematical and graphical methods. From ATR spectra, it was found that there are no such major interactions between gabapentin and excipients. A significant endothermal peak was seen in the DSC investigation at 208.81 °C. X-ray diffraction revealed that gabapentin was present in the crystalline form. Drug crystals and SLN were seen to be dispersed and scattered from Scanning Electron Microscope (SEM). The optimized formulation's particle size was found to be 203.4 nm, the Polydispersity Index (PI) of 0.426, and the zeta value of 16.5 mV; indicating stability. Following a lethal and chronic dosage of picrotoxin, the gabapentin-SLN exhibited a higher anticonvulsant efficacy, according to in vivo research on rats (p<0.05).
Conclusion: Compared to the Bicuculline model, the optimized SLN demonstrated superior outcomes regarding seizure initiation in the Picrotoxin-induced convulsion.
ADVANCING TOPICAL POSACONAZOLE DELIVERY: BOX BEHNKEN DESIGN MICROSPONGE HYDROGEL OPTIMIZATION AND EXTENSIVE IN VIVO INVESTIGATION
Authors: KUPPALA REKHA RANI , PRAKASH P. RAVI , DEVANNA N.
Published: November 2024

Objective: The primary aim of this study was to develop a novel hydrogel formulation containing Posaconazole (PCZ) encapsulated within microsponges. Furthermore, the study aimed to assess the permeation properties of this formulation in vivo using a mouse model.
Methods: To achieve this aim, a series of seventeen trials were conducted using the Box Behnken Design methodology. These trials were designed to optimize the production of PCZ Microsponges (PCZ MS), which were subsequently incorporated into a hydrogel matrix. Skin permeation studies were then performed to evaluate the ability of the PCZ microsponge-based hydrogel to deliver the drug across the skin barrier. These studies involved comparison with a standard hydrogel formulation lacking microsponges.
Results: This study assessed the efficacy of microsponge gel formulation PM-3 for drug entrapment, yield, and sustained release compared to a conventional gel. PM-3 displayed the highest entrapment efficiency of 98.5% and a yield of 95.62%, indicating a direct correlation with the 1:1 drug-polymer ratio. Moreover, PM-3 exhibited sustained drug release over 12 h, releasing 83.82% of PCZ compared to 65.31% with the normal gel, suggesting its potential for prolonged therapeutic action. These findings underscore the promise of microsponge-based hydrogels, like PM-3, in enhancing therapeutic outcomes through sustained drug release, warranting further exploration for clinical applications.
Conclusion: The findings of this study highlight the promising potential of microsponge-based hydrogels as effective carriers for localized drug delivery, particularly in the context of treating skin fungal infections.
POTENTIALITY OF PROTEIN HYDROLYSATE FROM ANADARA GRANOSA AS NUTRACEUTICAL AGENT: ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES
Authors: PERMATA YADE METRI , LAILA LIA , YULIASMI SRI , THERESIA LEWI , WIJAYA VIVIENNE
Published: November 2024

Objective: This study aims to extract protein hydrolysate from Anadara granosa and assess its impact on protein solubility, antioxidant, and antibacterial activities.
Methods: Several methods were used, including the isolation of enzyme bromelain, protein extraction from A. granosa, and the breakdown of protein using trypsin and bromelain. Together with its protein solubility, antioxidant activity (IC50) against free radicals (DPPH), the protein hydrolysate's antibacterial activity (MIC and inhibition zone) against Staphylococcus aureus and Escherichia coli was evaluated.
Results: With a molecular weight of 10 kDa and an IC50 of 83.81 mg/ml, the trypsin protein hydrolysate fraction showed remarkable antioxidant activity after 5 h of incubation. At a dosage of just 0.25 mg/ml, the A. granosa protein and its corresponding hydrolysate had inhibitory zones against S. aureus and E. coli that were comparable to those observed in samples treated with amoxicillin. Using trypsin as an enzyme for 3 or 5 h produced the strongest hydrolyzed product. The trypsin hydrolysate was better than the bromelain hydrolysate because of its antioxidant and antibacterial activities.
Conclusion: Based on the results, antioxidant and antibacterial activities, and protein solubility were influenced by enzymatic hydrolysis.
DESIGN, OPTIMIZATION AND EVALUATION OF RANOLAZINE FAST-DISSOLVING FILMS EMPLOYING MANGO KERNEL STARCH AS A NEW NATURAL SUPERDISINTEGRANT
Authors: DEVI MEDISETTY GAYATRI , R SANTOSH KUMAR
Published: November 2024

Objective: The BCS class II cardiovascular medication, Ranolazine (RZN), is characterized by limited solubility and inadequate oral absorption. The objective of the current research is to develop a natural superdisintegrant in the formulation of Fast-Dissolving Films (FDFs) of Cardio Vascular Drug (CVD) RZN to enhance its dissolution rate, solubility, absorption, and therapeutic action.
Methods: Mango Kernel Starch (MKS) is isolated by grinding the kernels, forming a slurry with water, filtering, and using repeated centrifugation and washing to purify the starch, which is then dried. The obtained starch is collected. Along with obtained natural superdisintegrant MKS, Maltodextrin (MDX) and Sodium Starch Glycolate (SSG) were also utilized in the fabrication of FDFs containing RZN via the solvent casting technique. A total of eight formulations (RF1 to RF8) were developed employing a 23 factorial design, using the natural superdisintegrant alone at a concentration of 5% and in combination with other superdisintegrants.
Results: The prepared MKS was found to be free-flowing, fine, amorphous, insoluble in organic solvents, and exhibiting 0.17% solubility in water with a swelling index of 89.95%, indicating superdisintegrant properties. Fourier-transform infrared spectroscopy (FTIR) studies and Differential scanning calorimetry (DSC) analysis indicated that there was no drug-excipient interaction. The films prepared with a 5% concentration of the MKS showed good physical properties and resulted in an increased drug dissolution rate, with 99.78 % of the drug dissolved within 10 min, along with the lowest disintegration time of 13.45 sec.
Conclusion: The research successfully isolated a new superdisintegrant, MKS and formulated FDFs of the poorly water-soluble drug RZN. The MKS was found to be an effective superdisintegrant with no drug interactions, producing films with good physical and mechanical properties, increasing the drug dissolution rate, and providing rapid disintegration with improved relative bioavailability.
FORMULATION, CHARACTERIZATION AND OPTIMIZATION OF PLGA-CHITOSAN-LOADED FATTY ACID SCAFFOLDS FOR THE TREATMENT OF DIABETIC WOUNDS
Authors: THUMBOORU SHILPA N. , AHMED SYED SUHAIB , HARI BALAJI , MP GOWRAV , REDDY KARRI VVS NARAYANA
Published: November 2024

Objective: The objective of the current research to formulate Eicosapentanoic Acid/Decosahexanoic Acid (EPA/DHA)incorporated into Chitosan (CS)and Poly-Lactic-Glycolic Acid (PLGA), nanoparticles composite scaffolds to the accelerated diabetic wound healing. The main focus of this present research is to evaluate and develop the chitosan–PLGA biodegradable polymer scaffolds loaded with long-chain omega-3 Polyunsaturated Fatty Acids (PUFA’s) (EPA/DHA).
Methods: Nano scaffolds were prepared by solvent evaporation method loaded with CS-PLGA, EPA and DHA to treat diabetic wounds at targeted site as pharmacotherapeutically. Upon investigation, the developed biodegradable crosslinked scaffold possesses matrix degradation, optimal porosity, prolonged drug release action than the non-cross linked scaffold. The prepared formulation containing CS-PLGA loaded with EPA/DHA were formulated as nanoscaffold for wound topical applications was carried out by using freeze drying process.
Results: The prepared CS-PLGA nano scaffolds were optimized and evaluated for physicochemical properties, dynamic light scattering with a particle size of 248 nm and zeta of-24mVand Scanning Electron Microscopy (SEM) were found to be spherical. In addition, the optical properties of EPA/DHA and PLGA, along with CS, can be compared by examining their absorption and wavelength (nm) using UV-visible spectroscopy. The structural and functional groups of the prepared end products were characterized by Fourier-Transformed Infrared Spectroscopy (FT-IR) has shown good compatibility with excipients and nanoformulaton, in vitro drug release studies done by using dialysis bag membrane results find that first-order Higuchi model was followed showing 20% release in first 0.2 h. MTT(3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide) assay was carried out and it showed that both crosslinked and non-crosslinked scaffolds(110 and 120%) improved cell growth when compared to control (100%).
Conclusion: Finally, the results showed that the PLGA, CS nanoscaffolds containing 98% of PUFA’s (EPA/DHA) have increased in proinflammatory cytokines production at the particular wound site and thus accelerated healing activity, depending on the pre-clinical studies have trespassed, the therapeutic potential to penetrating at wound site. The optimized nanoformulation could be a better formulation for targeting and treatment of diabetic wounds at an optimal ratio.
BOX-BEHNKEN DESIGN ASSISTED ECO-FRIENDLY RP-HPLC-PDA METHOD FOR THE QUANTIFICATION OF PACLITAXEL: APPLICATION TO EVALUATE THE SOLUBILITY OF PACLITAXEL-CYCLODEXTRIN COMPLEX
Authors: GUPTA ASHUTOSH , KOSSAMBE RIMA V. , MOORKOTH SUDHEER
Published: November 2024

Objective: Paclitaxel (PTX) is one of the oldest chemotherapeutic agents for cancer treatment. However, PTX is a class IV drug under the Biopharmaceutical Classification System (BCS), and its oral administration is restricted due to its low bioavailability. Complexing PTX with Beta-Cyclodextrin (β-CD) is an option to overcome the low solubility and bioavailability. This study aims to optimize and develop an RP-HPLC analytical method for quantifying PTX from the fabricated β-CD complex.
Methods: The HPLC settings were optimized using Design-of-Experiments (DOE) software. The independent variables for the optimization process were buffer ratio, buffer pH, flow rate, and injection volume. The responses were Retention Time (RT), peak area, Tailing Factor (TF), and number of Theoretical Plates (TP) of PTX. The validated method was then used to measure the % entrapment from the PTX-β-CD complex.
Results: The developed and optimized RP-HPLC method was validated as per International Council for Harmonisation (ICH) Q2 (R1) guidelines. The developed method showed linearity R2 = 0.999 with a 0.5-20 µg/ml range. The Limit of Detection (LOD) and Limit of Quantification (LOQ) were 95 and 125 ng/ml, respectively. The accuracy and precision for the developed method came under the acceptance criteria. The developed method was used to evaluate the enhancement of solubility of the prepared PTX-β-CD complex. The method was also used in the evaluation of % drug loading, % drug release and stability of the PTX-β-CD complex. The study clearly showed that the solubility of PTX increased from 0 to 1.14±0.53 µg/ml at pH 1.2 and 0 to 3.18±0.61 µg/ml at pH 6.8, respectively. The PTX-β-CD complex showed 73±3.75% drug release in 120 min at pH 1.2 and 87±3.51% at pH 6.8. The developed RP-HPLC method was found to be eco-friendly as per the Analytical Greenness (AGREE) metric approach and software analysis.
Conclusion: An eco-friendly RP-HPLC analytical method was successfully developed and optimized for the quantification of PTX from the PTX-β-CD complex.
PRELIMINARY STUDY ON ALGINATE CONCENTRATION AND ANTIBACTERIAL ACTIVITY OF PALMAROSA ESSENTIAL OIL
Authors: UTAMI NASTITI , PUSPITASARI DIAN , KUSUMA DEWI RETNANINGTYAS , LINDAWATI NOVENA YETY
Published: November 2024

Objective: Palmarosa (Cymbopogon martinii (Roxb.)) essential oil has volatile active compounds, therefore, it requires modification of encapsulation to obtain optimum potency. This study investigated the relationship between various alginate concentrations in microencapsulation against the quality of the formula and antibacterial activity.
Methods: The study use Palmarosa Essential Oil (PEO) that distillated at Rumah Atsiri, Indonesia. Ionic gelation was used to prepare microencapsulations at different alginate concentrations of 0.5%, 0.75%, and 1.5%. The investigation involved Fourier Transform Infrared (FTIR), organoleptic, morphological, microencapsulated weight, Encapsulation Efficiency (EE), and antibacterial activity.
Results: The organoleptic observation results for all formulas are white in color, have a pronounced PEO scent, and contain spherical particles with macrometer-sized morphology similar to soft beads. The result FTIR showed that F1, F2, and F3 contain aromatic ring, primarily alcohol, alkene, alkyl, and alcohol. The results showed that F1, F2, and F3 were included in the microencapsulation range, namely 5-5,000 µm. Formula III had the greatest EE of 86.53±0.75% and antibacterial activity against Staphylococcus epidermidis and Pseudomonas aeruginosa, respectively showed inhibition zones with diameters of 12.30±0.16 mm and 7.60±0.24 mm.
Conclusion: This study revealed that the findings of this study demonstrate that the concentration of alginate in microencapsulation influences the properties and antibacterial activity of PEO. Higher alginate concentrations can lead to increased EE, particle size distribution, and ultimately leading to enhanced antibacterial activity.
AGOMELATINE ETHOSOMES FOR ENHANCED TRANSDERMAL DRUG DELIVERY
Authors: RAJITHA NALLAGANDLA , DANIEL KOTHAPALLY
Published: November 2024

Objective: The current study aimed to prepare and optimize Agomelatine (AMN) ethosomes for enhanced transdermal drug delivery.
Methods: In this study cold method was employed to manufacture the AMN-loaded ethosomes with dissimilar quantities of Phosphatidyl Choline (PC): Cholesterol: Ethanol. Transmission Electron Microscopy (TEM) was employed to evaluate the appearance of the formed ethosomes. Other formulation parameters like vesicle size and zeta potential, polydispersity index, transition temperature, and entrapment efficiency were also investigated.
Results: The microscopy results showed that AMN ethosomes have a smooth surface. It was discovered that the AMN-3 formulation of transdermal ethosomes had 92.15±1.3 entrapment efficiency with good vesicle diameter. The release of agomelatine adhered to the zero-order release model. The polydispersity Index (PI) and zeta potential of the optimized formulation were found to be 0.209 and-14.09±1.95 mV, respectively. The maximum flux for the ethosome formulation (AMN-3) was 34.29 µg. h/cm2. A 10.71 fold increment was observed in the bioavailability of optimized formulation than control (oral suspension). A higher drug concentration in the blood suggested better systemic absorption of ethosomes. The optimized formula has a Tmax of 4.0±0.08h and 73.38±1.37 of Cmax. The AMN ethosomes were found to be more stable when stored at 4 °C.
Conclusion: The current study suggests that ethosomal vesicles may improve transdermal dispersion without causing skin irritation. Agomelatine-loaded ethosome has the potential to be one of the most important transdermal application techniques for the treatment of depression.
Konstruksi Identitas Naratif Konsumen Hijau dalam Gaya Hidup Nol Sampah
Authors: Budiana Egi , Prabasmoro Tisna , Adji Muhamad
Published: November 2024

Di tatanan masyarakat kontemporer, aktivitas konsumsi hijau dalam gaya hidup nol sampah berimplikasi pada pembentukan identitas konsumen. Menurut teori budaya konsumen, hal ini disebabkan nilai simbolis dan nilai kultural dari aktivitas konsumsi. Artikel ini bertujuan menjelaskan konstruksi identitas konsumen hijau di Kota Bandung dalam penerapan gaya hidup nol sampah di kehidupan sehari-hari. Untuk memahami konstruksi tersebut, artikel ini menggunakan metode wawancara life story yang akan dianalisis berlandaskan konsep identitas naratif dan teori processual identity. Informan terdiri dari lima pelanggan Toko Organis YPBB—suatu toko curah yang berfokus pada edukasi prinsip nol sampah. Penelitian ini menemukan dua dimensi dalam proses konstruksi identitas konsumen hijau. Pertama adalah dimensi refleksi yang menjelaskan afirmasi diri sebagai bentuk peneguhan identitas yang ditempuh lewat aktivitas konsumsi hijau. Kedua adalah dimensi interaksi sosial yang menampilkan negosiasi identitas di arena sosial informan.  Artikel ini berargumen bahwa pola konsumsi dalam gaya hidup dapat menegaskan identitas seseorang melalui apa yang orang lain persepsikan dan yang orang itu refleksikan tentang dirinya sendiri.
 
In contemporary society, green consumption activities on zero waste lifestyle imply consumer identity construction. Based on consumer culture theory, it happens due to the symbolic and cultural values from the consumption act. This article aims to explain the construction of a green consumer identity in Bandung on zero waste lifestyle implemented in everyday life. This article uses life story interview method and then will be analyzed by narrative identity concept and processual identity theory. The informants consist of five YPBB Toko Organis customers—it is a bulk store where people can learn about the zero waste principle. This research finds two dimensions of the green consumer identity construction process. First, the reflection dimension explains self-affirmation as a form of identity assertion through green consumption activities. Second, the social interaction aspect which displays identity negotiation processes in one social arena. This article argues that consumption schemes in a lifestyle affirms one’s identity through what others perceive and what one reflects about oneself.
Relevamiento y caracterización de propuestas que combinan el teatro y las ciencias en Argentina
Authors: Garcia de Souza Javier Ricardo , Garcia Pablo Facundo , Aramburú Rosana Mariel
Published: November 2024

En los últimos años en Argentina han surgido numerosas propuestas en las que tanto científicos/as como artistas combinan ciencia y escena, ya sea como forma de arte y/o de comunicación pública de la ciencia. Para conocer el estado actual de este campo de acción se realizó una investigación en la que a partir de una búsqueda exhaustiva, un relevamiento y entrevistas se buscó conocer qué acciones se realizan en nuestro país y quiénes, cómo y por qué llevan adelante estas prácticas, de lo que podría denominarse teatro científico, así como sus historias, intereses y percepciones acerca del mismo.
Potencial de Sensores Remotos en la Detección de Fosas Clandestinas
Authors: Mejía López Andrés Alejandro , Ochoa Gutiérrez Luis Hernán
Published: November 2024

En Colombia, la identificación de fosas comunes se ha convertido en una labor necesaria para el esclarecimiento de la verdad del conflicto violento. La actividad se realiza manualmente en la mayoría de los casos; para identificar los sitios de enterramiento se usa como insumo la información suministrada por los victimarios o los relatos de familiares de las víctimas. Sin embargo, estos métodos, además de imprecisos, son extremadamente tediosos y costosos. Para incrementar las posibilidades de éxito de dicha labor, en otros países se han experimentado métodos de búsqueda basados en resistividad geofísica, magnetometría o radar de penetración del suelo. Estos métodos solo pueden usarse en un área delimitada, a menudo pequeña, además de presentar retos si están en lugares de difícil acceso como ciénagas, pantanos o en lugares donde persiste el conflicto, lo que podría implicar que estén minados. Por consiguiente, existe la necesidad de implementar métodos que no impliquen acceso directo al terreno y que puedan abarcar áreas extensas, agilizando la búsqueda y reduciendo el costo en tiempo y en dinero. En este artículo se explora el uso de datos obtenidos a partir de sensores remotos para identificar cómo la salud de las plantas es afectada por el contenido de materia orgánica presente en las fosas comunes, correlacionando estas con el análisis de los índices espectrales NDVI, GNDVI y GCI. Para obtener imágenes sobre el área experimental, donde se simularon tumbas con materia orgánica de origen animal y restos óseos humanos, se utilizó el sensor multiespectral Sequoia Parrot, transportado en un UAV DJI Phantom 3 Advanced, con el cual se adquirieron imágenes en las longitudes de ondas Red, Green y nIR, para medir los índices de vegetación y estimar la salud de las plantas sobre y alrededor de las fosas. Los resultados obtenidos permitieron inferir los lugares donde se encuentran inhumados los cuerpos en el sitio experimental después de casi una década de haber sido creado el modelo experimental.
GROUP MEAN CORDIAL LABELING OF SOME QUADRILATERAL SNAKE GRAPHS
Authors: R N Rajalekshmi , R Kala
Published: November 2024

Let G be a (p, q) graph and let A be a group. Let f : V (G) −→ A be a map. For each edge uv assign the label [o(f (u))+o(f (v)) / 2]. Here o(f (u)) denotes the order of f (u) as an element of the group A. Let I be the set of all integerslabeled by the edges of G. f is called a group mean cordial labeling if the following conditions hold: (1) For x, y ∈ A, |vf (x) − vf (y)| ≤ 1, where vf (x) is the number of vertices labeled with x. (2) For i, j ∈ I, |ef (i) − ef (j)| ≤ 1, where ef (i) denote the number of edges labeled with i. A graph with a group mean cordial labeling is called a group mean cordial graph. In this paper, we take A as the group of fourth roots of unity and prove that, Quadrilateral Snake, Double Quadrilateral Snake, Alternate Quadrilateral Snake and Alternate Double Quadrilateral Snake are groupmean cordial graphs.
The Resident Scholars Program for Workforce Diversity: A Qualitative Study About Supporting Family Medicine Abortion Providers
Authors: Summit Aleza K. , Kirby Destiney , Carvajal Diana N.
Published: November 2024

Background and Objectives: While workforce diversity helps mitigate health inequities, few initiatives support prospective abortion providers who are underrepresented in medicine (URiM). To address this issue, Reproductive Health Education in Family Medicine established the Resident Scholars Program for Workforce Diversity (RSPWD), a year-long program for URiM and other Black, Indigenous, People of Color (BIPOC) residents committed to sexual and reproductive health (SRH) provision. Program elements include: (a) mentorship by BIPOC family physicians; (b) virtual didactic sessions about SRH integration into primary care, advocacy, leadership, reproductive justice, and patient-centered care; (3) conference sponsorship; and (4) community-building among residents and mentors.
Methods: We conducted a qualitative study with the program’s first cohort of residents and mentors to examine perspectives about program successes and needed improvements. We used a semistructured interview guide along with a direct contact analysis approach.
Results: We interviewed eight scholars and four mentors. From the interviews, we gleaned data on three main themes: (a) value of mentorship, (b) importance of community, and (c) program improvement suggestions. Scholars expressed appreciation for SRH mentorship from BIPOC mentors who had lived experiences similar to their own, noted the mentorship’s importance for career-building, and spoke positively of their sense of safe community among all program participants. Respondents shared suggestions for improved scheduling and requested better guidance for navigating the mentee–mentor relationship.
Conclusions: The RSPWD success is reflected in the enthusiasm and gratitude for the program and the resulting mentorship and community that fostered supportive personal and professional relationships, including career opportunities. When considering the importance yet dearth of workforce diversification in family medicine, this program offers a promising model for supporting a more diverse and representative future SRH workforce that may apply to other family medicine clinical niches.
Estate and War: The Motif of the Assembling of the “Universal House” in M.M. and V.D. Prishvins’ Works
Authors:   Orthodox St. Tikhon University for the Humanities, Knorre Elena Yu.
Published: November 2024

The article examines the “estate myth” in Mikhail Prishvin’s diaries of 1941–1945, in the book “You and I. Diary of Love” (1939–1940), written together with V.D. Prishvina, as well as in Valeria Prishvina’s memoirs “Our House” (1977). During the Second World War, the “estate myth” becomes part of Prishvin’s autobiographical myth, which is based on the motif of restoring the lost home, the parental estate of Khrushchevo-Levshino in Orel province. The search for home forms the creative attitude of the Prishvin family, where home is not a refuge, but a topos of salvation salvation for all that perishes, revelation of the joy of a transformed life. The space of the house during the war contains features of “heterotopia”. The image of a “home of happiness”, remote in the wilderness and at the same time included in common life, addressed to the suffering of his contemporaries, turns into the theme of the “universal home” as the creation of the world during the war already in the memoirs of V.D. Prishvina “Our House”. The article proves that the estate and dacha life of the Prishvins is not an immersion in nostalgia for the lost past, a kind of escapism in a catastrophic time of war, but conscious life practice, the so-called “estate habitus”, which mobilizes a person in a crisis period of history, awakening “kinship attention” and compassion for the Other, unites people into an “invisible community”, overcoming the spontaneous, destructive forces of war inside and outside the person.
“Heterotopia of the Estate” in I.A. Bunin’s Prose: from the First Story to the Novel “The Life of Arsenyev”
Authors:   Ural Federal University named after the first President of Russia B.N. Yeltsin, Prashcheruk Natalia V.
Published: November 2024

The article analyzes how the estate topos is presented in Bunin’s first story “Passion” (1897) and his novel “The Life of Arsenyev” (1933). Describing three estates in his first story, young Bunin gives a panoramic image of Russian estate life at the end of the 19th century in its variations — from an ancient manorial estate and an advanced landowner’s farm to a small farm estate. “Passion” makes an attempt to outline a collective image of a person of the estate type and show that there is something common that unites all persons of the estate type — attachment to their native nest, loyalty to their roots, the desire to preserve these roots. In “The Life of Arsenyev” the “estate topos” is voluminous and heterogeneous, represented by a whole system of vectors, and acquires the character of a heterotopia. The vectors analyzed are everyday, natural, national, literary, mythopoetic, existential, and sacred vectors. With general inclusion in the “estate topos”, each vector has its own semantic and artistic specificity. For a writer, an estate is the optimal way to arrange a place of life for a Russian person. The writer expresses the idea that all of Russia is not only a village, but also an estate. “The Life of Arsenyev” constructs a whole estate cosmos — in national, cultural and sacred aspects; the face of Russia is presented here as metaphysically enlightened, and this light extends to the estate culture, which is timeless.
Elements of the “Estate Text” in the Novel “Paths of Heaven” by I.S. Shmelev
Authors:   Tula State Lev Tolstoy Pedagogical University, Abramova Veronika I.
Published: November 2024

The article studies how the elements of the “estate text” function in the novel “Paths of Heaven” by I.S. Shmelev. The author of the study, based on the typology of V.G. Shchukin, finds and characterizes these elements in the structure of the literary work. In the second volume of the novel, one can find motifs, images, details, plot twists characteristic of the “estate text”: arrival at the native nest, a fresh morning the next day, images of an old house, a library, a linden alley, a flower garden, an old ser- vant, a pure spiritualized girl, a motive of gratifying nostalgic sadness, etc. The “estate text” of Shmelev is supported by the Turgenev context: the space, in which the action takes place in the second volume, is the Tula — Oryol places where “The Hunter’s Sketches” were created, where Spasskoye-Lutovinovo and Turgenevo are located. For the emigrant writer, this topos has a special semantics denoting the lost homeland. The article concludes that the elements of the “estate text” play a symbolic role in the ideological aspect of the novel: through the estate space, the characters move towards spiritual salvation, towards inner rebirth. In this regard, in the artistic structure of the “Paths of Heaven”, the “estate text” undergoes a kind of semantic inversion: the estate is conceived not as a paradise lost or being lost, but on the contrary, as a paradise found, as a gift for the suffering experienced and fidelity to God.