bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuroanatomical changes of ionotropic glutamatergic and GABAergic receptor densities in male mice modeling idiopathic and syndromic autism spectrum disorder
Authors: Nardi Leonardo , Chhabra Stuti , Leukel Petra , Krueger-Burg Dilja , Sommer Clemens J. , Schmeisser Michael J.
Published: July 2023

Autism spectrum disorder (ASD) comprises a wide range of neurodevelopment conditions primarily characterized by impaired social interaction and repetitive behavior, accompanied by a variable degree of neuropsychiatric characteristics. Synaptic dysfunction is undertaken as one of the key underlying mechanisms in understanding the pathophysiology of ASD. The excitatory/inhibitory (E/I) hypothesis is one of the most widely held theories for its pathogenesis. Shifts in E/I balance have been proven in several ASD models. In this study, we investigated three mouse lines recapitulating both idiopathic (the BTBR strain) and genetic (Fmr1 and Shank3 mutants) forms of ASD at late infancy and early adulthood. Using receptor autoradiography for ionotropic excitatory (AMPA and NMDA) and inhibitory (GABAA) receptors, we mapped the receptor binding densities in brain regions known to be associated with ASD such as prefrontal cortex, dorsal and ventral striatum, dorsal hippocampus, and cerebellum. The individual mouse lines investigated show specific alterations in excitatory ionotropic receptor density, which might be accounted as specific hallmark of each individual line. Across all the models investigated, we found an increased binding density to GABAA receptors at adulthood in the dorsal hippocampus. Interestingly, reduction in the GABAA receptor binding density was observed in the cerebellum. Altogether, our findings suggest that E/I disbalance individually affects several brain regions in ASD mouse models and that alterations in GABAergic transmission might be accounted as unifying factor.
Sex difference in the association between blood alcohol concentration and serum ferritin
Authors: Yehia Asmaa , Sousa Ricardo A. L. , Abulseoud Osama A.
Published: July 2023

Introduction

The sex difference in alcohol use disorder (AUD) is ingrained in distinctive neurobiological responses between men and women, which necessitates further investigation for a more tailored management.

Methods

Minding the findings of iron dysregulation in AUD and the sex difference in iron homeostasis in multiple physiological and pathological settings, we examined the sex difference in the association between serum ferritin and blood alcohol concentration (BAC) in intoxicated males (n = 125) and females (n = 59). We included patients with both serum ferritin tested of any value and a BAC above the level of detection during the same hospital admission period. We investigated sex difference in the relationship between BAC, serum ferritin and liver enzymes in intoxicated critically ill and noncritically ill patients.

Results

We found a negative association between serum ferritin and BAC in critically ill, intoxicated females [R2 = 0.44, F(1,14) = 11.02, p = 0.005], with much attenuated serum ferritin in females compared to their male counterparts (194.5 ± 280.4 vs. 806.3 ± 3405.7 ng/L, p = 0.002). We found a positive association between serum ferritin and liver enzymes [alanine transaminase (ALT) and aspartate transferase (AST)] in critically ill intoxicated females [ALT: R2 = 0.48, F(1,10) = 9.1, p = 0.013; AST: R2 = 0.68, F(1,10) = 21.2, p = 0.001] and in noncritically ill intoxicated males [ALT: R2 = 0.1, F(1,83) = 9.4, p = 0.003; AST: R2 = 0.1, F(1,78) = 10.5, p = 0.002]. The effect of BAC on serum ferritin was not mediated by ALT [indirect effect: (B = 0.13, p = 0.1)]. We also found a significant effect of sex, anemia, intensive care unit (ICU) admission and mortality on serum ferritin.

Discussion

Our results suggest that high BAC in intoxicated female patients is associated with attenuated serum ferritin levels, questioning the role of low serum ferritin in female vulnerability to alcohol.
Impact of smart device use on objective and subjective health of older adults: findings from four provinces in China
Authors: Wei Yan , Guo Xinyu
Published: July 2023

Background

The digital divide has grown because of the deepening digitalization of the Chinese society and the intersection between high-end technology and the age structure. Older adults show an increasing level of weakness in digital life integration. What digital development can bring to older adults is a pressing concern.

Objective

This study aims to investigate how smart device use affects older adults' health status and offers an empirical reference for improving their digital literacy and health.

Methods

The data in this study were collected from an offline survey conducted from December 2021 to April 2022, which obtained 1110 valid samples of older adults This study used a multivariate ordered logit model, mediating effect model, and heterogeneity test to analyze the impact of smart device use on the health status of older adults.

Results

Smart device use has a significant positive effect on the self-rated, physical, and psychological health of older adults, and this positive effect is more pronounced among older adults living in urban areas or with a higher age. The average net effect of smart devices on each health status of older adults is 0.161 for self-rated health, 0.155 for physical health, and 0.071 for psychological health. In-depth research found that older adults' attitudes toward smart devices played a mediating role in the influence of smart device use on self-rated and psychological health respectively.

Conclusions

The study found that smart device use had a positive effect on the health status of older adults and that the performance expectations and individualized needs of older adults exhibited an effective linkage between smart devices and health status. Smart device use could improve the overall health of older adults, especially the urban and low-age older adults. Promoting the understanding of the practicality of smart devices for older adults and the aging-oriented transformation of smart devices is an effective way to improve the health status of older adults. The findings provide theoretical support for the wide application of smart devices in older adults, and can effectively help eHealth practitioners implement accurate geriatric health support strategies.
Association between organic cation transporter genetic polymorphisms and metformin response and intolerance in T2DM individuals: a systematic review and meta-analysis
Authors: Peng Aiyu , Gong Chunmei , Xu Yuanfei , Liang Xiongshun , Chen Xiaoping , Hong Wenxu , Yan Junxia
Published: July 2023

Background

Variants in organic cation transporter (OCT) genes play a crucial role in metformin pharmacokinetics and are critical for diabetes treatment. However, studies investigating the effect of OCT genetic polymorphisms on metformin response have reported inconsistent results. This review and meta-analysis aimed to evaluate the associations between OCT genetic polymorphisms and metformin response and intolerance in individuals with type 2 diabetes mellitus (T2DM).

Method

A systematic search was conducted on PubMed, EMBASE, CNKI, WANFANG DATA, and VIP database for identifying potential studies up to 10 November 2022. The Q-Genie tool was used to evaluate the quality of included studies. Pooled odds ratios (OR) or standardized mean differences (SMD) and 95% confidence intervals (95% CI) were calculated to determine the associations between OCT genetic polymorphisms and metformin response and intolerance that were reflected by glycemic response indexes, such as glycated hemoglobin level (HbA1c%) or change in glycated hemoglobin level (ΔHbA1c%), fasting plasma level (FPG) or change in fasting plasma glucose level (ΔFPG), the effectiveness rate of metformin treatment, and the rate of metformin intolerance. A qualitative review was performed for the variants identified just in one study and those that could not undergo pooling analysis.

Results

A total of 30 related eligible studies about OCT genes (SLC22A1, SLC22A2, and SLC22A3) and metformin pharmacogenetics were identified, and 14, 3, and 6 single nucleotide polymorphisms (SNPs) in SLC22A1, SLC22A2, and SLC22A3, respectively, were investigated. Meta-analysis showed that the SLC22A1 rs622342 polymorphism was associated with a reduction in HbA1c level (AA vs. AC: SMD [95% CI] = −0.45 [−0.73–−0.18]; p = 0.001). The GG genotype of the SLC22A1 rs628031 polymorphism was associated with a reduction in FPG level (GG vs. AA: SMD [95 %CI] = −0.60 [−1.04–0.16], p = 0.007; GG vs. AG: −0.45 [−0.67–0.20], p < 0.001). No statistical association was found between the remaining variants and metformin response and intolerance.

Conclusion

SLC22A1 rs622342 and rs628031 polymorphisms were potentially associated with glycemic response to metformin. This evidence may provide novel insight into gene-oriented personalized medicine for diabetes.
Health promotion programs in prison: attendance and role in promoting physical activity and subjective health status
Authors: Tesler Riki , Regev Ofer , Birk Ruth , Barak Sharon , Shapiro Yair , Weiss Yossi , Zigdon Avi , Ben Zvi Kathrin , Vaknin Yochanan , Green Gizell , Sohlberg Idit , Zwilling Moti , Goldstein Liav
Published: July 2023

Introduction

Maintaining an inmate’s health can serve as a challenge due to unhealthy background, risky behavior, and long imprisonment. This study aimed to analyze the prevalence of participation in health promotion activities among Israeli inmates and its association with their physical activity levels and subjective health status.

Methods

A cross-sectional study was designed to examine 522 inmates (429 males, 93 females). The data were collected by trained face-to-face interviewers and self-report questionnaires.

Results

Most of the participants (82.37%) did not meet the recommended physical activity level. Half of the participants reported that their physical activity levels decreased since they were in prison compared with 29.50% who reported that their physical activity levels increased. Physical activity and subjective health status were significantly higher among younger male inmates. Furthermore, participation in health-promoting activities was associated with higher levels of physical activity and subjective health status.

Discussion

Health promotion activities may play an important role in addressing the challenges of maintaining inmate health. Implications of the findings are further discussed.
Forest gap effects on snow storage in the transitional climate of the Eastern Cascade Range, Washington, United States
Authors: Dickerson-Lange Susan E. , Howe Emily R. , Patrick Kenna , Gersonde Rolf , Lundquist Jessica D.
Published: July 2023

Forest thinning and gap creation are being implemented across the western United States of America (USA) to reduce wildfire and forest mortality risk as the climate warms. The Eastern Cascades in Washington, USA, is in a transitional zone between maritime and continental climate conditions and represents a data gap in observations describing the relationship between forest density and snowpack. We collected 3 years of snow observations across a range of forest densities to characterize how forest management efforts in this region may influence the magnitude and duration of snow storage. Observations indicate that peak snow storage magnitude in small gaps ranges from the same to over twice that observed in unburned forest plots in the Eastern Cascades. However, differences in snow duration are generally small. Across all Eastern Cascade sites and years, we observed a median difference of snow storage lasting 7 days longer in gaps as compared to nearby forest plots. A notable exception to this pattern occurred at one north-facing site, where snow lasted 30 days longer in the gap. These observations of similar snow storage duration in the Eastern Cascades are attributed to minimal differences in canopy snow interception processes between forests and gaps at some sites, and to higher ablation rates that counterbalance the higher snow accumulation in the gaps at other sites. At the north-facing site, more snow accumulated in the gap, and ablation rates in the open gap were similar to the shaded forest due to the aspect of the site. Thus, snow storage duration was much longer in the gap. Together, these data suggest that prescriptions to reduce forest density through thinning and creating gaps may increase the overall amount of snow storage by reducing loss due to sublimation and melting of canopy-intercepted snow. However, reducing forest density in the Eastern Cascades is unlikely to buffer climate-induced shortening of snow storage duration, with the possible exception of gap creation in north-facing forests. Lastly, these observations fill a spatial and climatic data gap and can be used to support hydrological modeling at spatial and temporal scales that are relevant to forest management decisions.
Estimation of greenhouse gas emissions from Japanese healthy meals with different protein sources
Authors: Sameshima Himeno , Akamatsu Rie , Hayashi Fumi , Takemi Yukari
Published: July 2023

Introduction

Diets that promote people’s health and environment-friendly are essential for achieving a sustainable society. Protein sources are the main contributors of greenhouse gas emissions (GHGE), and lower intakes of livestock meat and more intakes of poultry meat and legumes are recommended. Although Japanese consume less meat than other countries, it is unclear whether the GHGE of healthy Japanese meals is sufficient to solve climate change. In addition, most previous studies have focused on general household meals, not necessarily healthy meals. Therefore, we explored recommended food choices of protein sources in both healthy and environment-friendly meals.

Methods

We used data on healthy meals provided by retailers certified under the “Healthy Meal and Food Environment” Certification System. We first examined the number of main ingredients in the staple, main, and side dishes. We then compared the GHGE of meals with different combinations of main ingredients of main dishes (protein sources). To estimate the GHGE, we developed a database of GHGE per food weight for each food in the Standard Tables of Food Composition in Japan.

Results

Data on a total of 509 meals were considered in the analysis. The mean ± standard deviation of the total GHGE of one meal was 1044.7 ± 614.9 g-CO2 eq/650 kcal. The minimum and maximum values were 412.5 and 4268.5 g-CO2 eq/650 kcal, respectively. Regarding meat, chicken was more likely to be used in meals with low GHGE.

Discussion

The healthy meals with the lowest GHGE in this study had the potential to contribute to solving climate change. Although healthy meals in this study were created with the same nutrient level criteria, a large difference existed between the minimum and maximum GHGE and it depends on the choice of protein ingredients. The findings may be useful to develop food guide for Japanese taking environmental perspectives into account.
Two Medoid-Based Algorithms for Clustering Sets
Authors: Nigro Libero , Fränti Pasi
Published: July 2023

This paper proposes two algorithms for clustering data, which are variable-sized sets of elementary items. An example of such data occurs in the analysis of a medical diagnosis, where the goal is to detect human subjects who share common diseases to possibly predict future illnesses from previous medical history. The first proposed algorithm is based on K-medoids and the second algorithm extends the random swap algorithm, which has proven to be capable of efficient and careful clustering; both algorithms depend on a distance function among data objects (sets), which can use application-sensitive weights or priorities. The proposed distance function makes it possible to exploit several seeding methods that can improve clustering accuracy. A key factor in the two algorithms is their parallel implementation in Java, based on functional programming using streams and lambda expressions. The use of parallelism smooths out the O(N2) computational cost behind K-medoids and clustering indexes such as the Silhouette index and allows for the handling of non-trivial datasets. This paper applies the algorithms to several benchmark case studies of sets and demonstrates how accurate and time-efficient clustering solutions can be achieved.
Be Innovative and Resilient: Empirical Evidence from Saudi Firms on How to Translate Entrepreneurial Orientation into Firm Performance
Authors: Aloulou Wassim J.
Published: July 2023

This research seeks to investigate the relationship between entrepreneurial orientation dimensions (behavioral dimension EOBD and attitudinal dimension EOAD) and firm performance (FP) through a sequential mediation model of innovation capability (IC) and firm resilience capability (FRC) in a specific context. Sample data for this study were collected using a questionnaire survey from 225 randomly selected SMEs in Saudi Arabia and analyzed using structural equation modeling. The results revealed that there are significant relationships between EO dimensions and IC. No direct relationships were found between EO dimensions and FRC or between EOAD and FP. Therefore, IC plays a mediating role in the relationships between EO dimensions and FRC. In addition, FRC does not play a mediating role in the relationships between EO dimensions and FP. On the contrary, it plays a partial mediation between IC and FP. To our knowledge, little research has investigated simultaneously the effects of EO, IC, FRC and FP in the Saudi context. Our study contributes to the literature on entrepreneurship, innovation and resilience by providing new empirical evidence. It also contributes to managerial practices by displaying the importance of translating strategic EO dimensions into performance outcomes through IC and FRC.
Variable-Weighted Error Propagation Model of a Ultra-Wide-Band Indoor Positioning System in an Intelligent Manufacturing Lab
Authors: Zhang Zhishu , Zhao Rongyong , Zhang Hao , Zhu Wenjie , Jia Ping , Li Cuiling , Ma Yunlong
Published: July 2023

Ultra-wide-band (UWB) positioning is a satisfying indoor positioning technology with high accuracy, low transmission cost, high speed, and strong penetration capacity. However, there remains a lack of systematic study on inevitable and stochastic errors caused by factors originating from the multipath effect (ME), non-line-of-sight interference (NLOSI), and atmospheric interference (AI) in UWB indoor positioning systems. To address this technical issue, this study establishes a dynamic error-propagation model (DEPM) by mainly considering the ME, NLOSI, and AI. First, we analyze the UWB-signal generation principle and spread characteristics used in indoor positioning scenarios. Second, quantization models of the ME, NLOSI, and AI error factors are proposed based on data from related studies. Third, to adapt to various environments, we present a variable-weighted DEPM based on the quantization models above. Finally, to validate the proposed dynamic error-propagation model, UWB-based positioning experiments in an intelligent manufacturing lab were designed and conducted in the form of static and dynamic longitude-tag position measurements. The experimental results showed that the main influencing factors were ME and NLOSI, with a weight coefficient of 0.975, and AI, with a weight coefficient of 0.00025. This study proposes a quantization approach to main error factors to enhance the accuracy and precision of indoor UWB-positioning systems used in intelligent manufacturing areas.
Modelling and Predicting Self-Compacting High Early Age Strength Mortars Properties: Comparison of Response Models from Full, Fractioned and Small Central Composite Designs
Authors: Cangussu Nara , Matos Ana Mafalda , Milheiro-Oliveira Paula , Maia Lino
Published: July 2023

The mixture design of cement-based materials can be complex due to the increasing number of constituent raw materials and multiple requirements in terms of engineering performance and economic and environmental efficiency. Designing experiments based on factorial plans has shown to be a powerful tool for predicting and optimising advanced cement-based materials, such as self-compacting high-early-strength cement-based mortars. Nevertheless, the number of factor interactions required for factor scheduling increases considerably with the number of factors. Consequently, the probability that the interactions do not significantly affect the answer also increases. As such, fractioned factorial plans may be an exciting option. For the first time, the current work compares the regression models and the predicting capacity of full, fractionated (A and B fractions) and small factorial designs to describe self-compacting high-early-strength cement-based mortars’ properties, namely, the funnel time, flexure and compressive strength at 24 h for the function of the mixture parameters Vw/Vc, Sp/p, Vw/Vp, Vs/Vm and Vfs/Vs for the different factorial designs. We combine statistical methods and regression analysis. Response models were obtained from the full, fractionated, and small plans. The full and fractionated models seem appropriate for describing the properties of self-compacting high-early-strength cement-based mortars in the experimental region. Moreover, the predicting ability of the full and fractionated factorial designs is very similar; however, the small design predictions reveal some concerns. Our results confirm the potentiality of fractioned plans to reduce the number of experiments and consequently reduce the cost and time of experimentation when designing self-compacting high-early-strength cement-based mortars.
The In-House Method of Manufacturing a Low-Cost Heat Pipe with Specified Thermophysical Properties and Geometry
Authors: Rogowski Michał , Fabrykiewicz Maciej , Szymański Paweł , Andrzejczyk Rafał
Published: July 2023

Various types of heat pipes are available to purchase off the shelf, from various manufacturers, but most of them have strictly defined geometry and technical parameters. However, when there is a need to use a heat pipe (HP) with an unusual size and shape or working conditions other than the standard ones, it becomes very costly to order them from manufacturers, especially in small quantities, and only a few producers are willing to fulfill such an order. This paper presents a detailed description and step-by-step method of manufacturing and testing a low-cost HP with specific properties and geometry, cooperating with a modular heat recovery system based on the use of phase change materials (PCM) for electromobility applications. The presented heat pipes were made entirely by hand, primarily with the use of basic workshop tools, without the use of specialized and automated CNC machines. Utensils used during the process were either made by hand or using desktop FDM 3D printers. During the evaluation of heat pipes’ performance within PCM (coconut oil), simple statistical functions were used. One-dimensional and two-dimensional histograms were used to visualize data obtained during this research. The presented method allows the manufacturing of heat pipes that are, on average, able to melt about 35% more PCM than an empty copper pipe with the exact same geometry. The HPs’ performance in coconut oil was evaluated on the basis of their future applications.
Volatility of a Ship’s Emissions in the Baltic Sea Using Modelling and Measurements in Real-World Conditions
Authors: Kangasniemi Oskari , Simonen Pauli , Moldanová Jana , Timonen Hilkka , Barreira Luis M. F. , Hellén Heidi , Jalkanen Jukka-Pekka , Majamäki Elisa , D’Anna Barbara , Lanzafame Grazia , Temime-Roussel Brice , Mellqvist Johan , Keskinen Jorma , Dal Maso Miikka
Published: July 2023

Shipping emissions are a major source of particulate matter in the atmosphere. The volatility of gaseous and particulate phase ship emissions are poorly known despite their potentially significant effect on the evolution of the emissions and their secondary organic aerosol (SOA) formation potential. An approach combining a genetic optimisation algorithm with volatility modelling was used on volatility measurement data to study the volatility distribution of a ship engine’s emissions in real-world conditions. The fuels used were marine gas oil (MGO) and methanol. The engine was operated with 50% and 70% loads with and without active NOx after-treatment with selective catalytic reduction (SCR). The volatility distributions were extended to higher volatilities by combining the speciation information of the gas phase volatile organic compounds with particle phase volatility distributions and organic carbon measurements. These measurements also provided the emission factors of the gas and particle phase emissions. The results for the particle phase volatility matched well with the existing results placing most of the volatile organic mass in the intermediate volatile organic compounds (IVOC). The IVOCs also dominated the speciated gas phase. Partitioning of the emissions in the gas and particle phases was affected significantly by the total organic mass concentration, underlining the importance of the effect of the dilution on the phase of the emissions.
Study of Archaeal Diversity in the Arctic Meltwater Lake Region
Authors: Qin Yiling , Wang Nengfei , Zheng Li , Li Qinxin , Wang Long , Xu Xiaoyu , Yin Xiaofei
Published: July 2023

Two typical lakes formed from meltwater in the Ny-Ålesund area were taken as the study subjects in 2018. To investigate the archaeal community compositions of the two lakes, 16S rRNA genes from soil samples from the intertidal and subtidal zones of the two lakes were sequenced with high throughput. At the phylum level, the intertidal zone was dominated by Crenarchaeota and the subtidal zone was dominated by Halobacter; at the genus level, the intertidal zone was dominated by Nitrososphaeraceae_unclassified and Candidatus_Nitrocosmicus, while the subtidal zone was dominated by Methanoregula. The soil physicochemical factors pH, moisture content (MC), total organic carbon (TOC), total organic nitrogen (TON), nitrite nitrogen (NO2−-N), and nitrate nitrogen (NO3−-N) were significantly different in the intertidal and subtidal zones of the lake. By redundancy analysis, the results indicated that NH4+-N, SiO32−-Si, MC, NO3−-N, and NO2−-N have had highly significant effects on the archaeal diversity and distribution. A weighted gene co-expression network analysis (WGCNA) was used to search for hub archaea associated with physicochemical factors. The results suggested that these physicochemical factors play important roles in the diversity and structure of the archaeal community at different sites by altering the abundance of certain hub archaea. In addition, Woesearchaeales was found to be the hub archaea genus at every site.
An Eco-Friendly Approach Utilizing Green Synthesized Titanium Dioxide Nanoparticles for Leather Conservation against a Fungal Strain, Penicillium expansum AL1, Involved in the Biodeterioration of a Historical Manuscript
Authors: Fouda Amr , Abdel-Nasser Mahmoud , Eid Ahmed M. , Hassan Saad El-Din , Abdel-Nasser Aya , Alharbi Nada K. , AlRokban Ahlam H. , Abdel-Maksoud Gomaa
Published: July 2023

The main hypothesis of the present research is investigating the efficacy of titanium oxide nanoparticles (TiO2-NPs) to prevent the growth of fungal strains when applied on leather under an experimental study. Therefore, fifteen fungal strains were isolated from a deteriorated historical manuscript (papers and leathers) and identified by traditional methods and ITS sequence analysis, including Aspergillus chevalieri (one isolate), A. nidulans (two strains), A. flavus (four strains), A. cristatus (one strain), A. niger (one strain), Paecilomyces fulvus (two strains), Penicillium expansum (two strains), and P. citrinum (two strains). The enzymes cellulase, amylase, pectinase, and gelatinase, which play a crucial role in biodegradation, were highly active in these fungal strains. TiO2-NPs were formed using the cell-free filtrate of the probiotic bacterial strain, Lactobacillus plantarum, and characterized. Data showed that the TiO2-NPs were successfully formed with a spherical shape and anatase phase with sizes of 2–8 nm. Moreover, the EDX analysis revealed that the Ti and O ions occupied the main component with weight percentages of 41.66 and 31.76%, respectively. The in vitro cytotoxicity of TiO2-NPs toward two normal cell lines, WI38 and HFB4, showed a low toxicity effect against normal cells (IC50 = 114.1 ± 8.1µg mL−1 for Wi38, and 237.5 ± 3.5µg mL−1 for HFB4). Therefore, concentrations of 100 μg mL−1 were used to load on prepared leather samples before inoculation with fungal strain P. expansum AL1. The experimental study revealed that the loaded TiO2-NPs have the efficacy to inhibit fungal growth with percentages of 73.2 ± 2.5%, 84.2 ± 1.8%, and 88.8 ± 0.6% after 7, 14, and 21 days, respectively. Also, the analyses including SEM, FTIR-ART, color change, and mechanical properties for leather inoculated with fungal strain AL1 in the absence of NPs showed high damage aspects compared to those inoculated with fungal strains in the presence of TiO2-NPs.
Chemical Composition of the Essential Oils of Three Popular Sideritis Species Cultivated in Greece Using GC-MS Analysis
Authors: Kaparakou Eleftheria H. , Daferera Dimitra , Kanakis Charalabos D. , Skotti Efstathia , Kokotou Maroula G. , Tarantilis Petros A.
Published: July 2023

(1) Background: The essential oils (EOs) of Sideritis L. have attracted great interest due to their pharmacological activities and potential applications in the cosmetic and perfume industries. The aim of this work was to study the EO chemical composition of three of the most popular, in Greece, mountain tea species: namely, these include Sideritis scardica, Sideritis raeseri, and Sideritis syriaca. (2) Methods: The EOs were obtained from the aerial parts of three Sideritis species that were cultivated in various regions of Greece by hydrodistillation, and the chemical composition was studied by gas chromatography–mass spectrometry (GC-MS) analysis. (3) Results: The EOs of the Sideritis species—S. scardica (SSC1, SSC2, SSC3), S. raeseri (SR1, SR2, SR3), and S. syriaca (SS1, SS2, SS3)—were analyzed by GC-MS, and they showed both qualitatively and quantitatively high variation in their chemical composition. (4) Conclusions: The EOs of S. scardica and S. raeseri from three different regions of Greece, and the S. syriaca from three different localities of Crete Island in Southern Greece, showed high chemical variability. Although 165 different components were found to be present in the nine samples through GC-MS analysis, only 7 (1-octen-3-ol, linalool, trans-pinocarveol, p-mentha-1,5-dien-8-ol, α-terpineol, myrtenol, and verbenone) were common components in the nine EOs, which were identified to be highly variable in different percentages among the samples. Even the EOs of SS1 and SS2, which were cultivated nearby, showed different GC profiles. The composition variation observed might be attributed to differentiations in the soil and climatic conditions.
A Novel Bio-Inspired Optimization Algorithm Design for Wind Power Engineering Applications Time-Series Forecasting
Authors: Karim Faten Khalid , Khafaga Doaa Sami , Eid Marwa M. , Towfek S. K. , Alkahtani Hend K.
Published: July 2023

Wind patterns can change due to climate change, causing more storms, hurricanes, and quiet spells. These changes can dramatically affect wind power system performance and predictability. Researchers and practitioners are creating more advanced wind power forecasting algorithms that combine more parameters and data sources. Advanced numerical weather prediction models, machine learning techniques, and real-time meteorological sensor and satellite data are used. This paper proposes a Recurrent Neural Network (RNN) forecasting model incorporating a Dynamic Fitness Al-Biruni Earth Radius (DFBER) algorithm to predict wind power data patterns. The performance of this model is compared with several other popular models, including BER, Jaya Algorithm (JAYA), Fire Hawk Optimizer (FHO), Whale Optimization Algorithm (WOA), Grey Wolf Optimizer (GWO), and Particle Swarm Optimization (PSO)-based models. The evaluation is done using various metrics such as relative root mean squared error (RRMSE), Nash Sutcliffe Efficiency (NSE), mean absolute error (MAE), mean bias error (MBE), Pearson’s correlation coefficient (r), coefficient of determination (R2), and determination agreement (WI). According to the evaluation metrics and analysis presented in the study, the proposed RNN-DFBER-based model outperforms the other models considered. This suggests that the RNN model, combined with the DFBER algorithm, predicts wind power data patterns more effectively than the alternative models. To support the findings, visualizations are provided to demonstrate the effectiveness of the RNN-DFBER model. Additionally, statistical analyses, such as the ANOVA test and the Wilcoxon Signed-Rank test, are conducted to assess the significance and reliability of the results.
Changes in Plasma TPH2, GDNF, Trk-b, BDNF, and proBDNF in People Who Died by Suicide
Authors: Liu Xiaoyu , Li Shangda , Yu Yueran , Hu Jianbo , Xu Yi
Published: July 2023

Recent studies have shown that neuropeptides and neurotrophic factors may be involved in the pathophysiological mechanisms of suicide. However, the current research on this aspect is still insufficient. Our study aimed to explore the biological patterns of suicide deaths, including levels of BDNF, proBDNF, BDNF/proBDNF, Trk-b, GDNF, and TPH2. The researchers selected 25 normal control patients matched by age with 30 suicide deaths. We used enzyme-linked immunosorbent assays to detect the levels of BDNF, proBDNF, BDNF/proBDNF, Trk-b, GDNF, and TPH2 in the plasma of suicide and control subjects. proBDNF, BDNF/proBDNF, Trk-b, GDNF, and TPH2 levels are shown as the median (25th–75th percentile). BDNF levels are shown as the mean (standard error of the mean). (1) The levels of plasma TPH2 and proBDNF in people who died by suicide were significantly higher than those in the control group. (2) The plasma levels of GDNF and BDNF/proBDNF in the suicide group were obviously lower than those in the control group. (3) There was no significant difference in plasma BDNF or Trk-b concentrations between the suicide group and the control group.Plasma TPH2, GDNF, and proBDNF levels are related to suicide. Plasma neurotrophic factor markers may predict suicide risk.
Investigating Vitreous Cytokines in Choroidal Melanoma
Authors: Demirci Hakan , Tang Lu , Demirci F. Yesim , Ozgonul Cem , Weber Sarah , Sundstrom Jeffrey
Published: July 2023

Due to the close relationship between the vitreous and posterior eye layers, the microenvironment of these layers can affect the composition of the vitreous. Molecular analysis of the vitreous may therefore provide important insights into the pathogenesis of chorioretinal diseases. In this study, vitreous cytokines (n = 41) were evaluated to gain further insights into the tumor microenvironment in uveal melanoma (UM) arising from the choroid (CM). Cytokine levels were measured using a bead-based multiplex immunoassay panel in vitreous samples obtained from 32 eyes, including 18 with CM and 14 controls. Median fluorescence intensity values were extracted and used as relative quantification of the cytokine abundance. Vitreous cytokine levels were compared between the CM and non-CM groups and between different prognostic categories within the CM group (classified as having low or high metastatic risk using tumor biopsy-based gene expression profiling). Correlations between vitreous cytokine levels and tumor dimensions were also evaluated. Our analysis revealed twenty-six vitreous cytokines significantly upregulated in CM-affected eyes compared to the control eyes. Within the CM group, six vitreous cytokines showed altered levels (five upregulated and one downregulated) in eyes with high- vs. low-risk tumors. Levels of these six plus several other cytokines showed correlations with the tumor dimensions. In conclusion, our study has uncovered several UM-relevant vitreous cytokines, worthy of follow-up in larger studies as potential candidates for liquid biopsy-based biomarker development and/or new therapeutic targeting.
Influence of [18F]FDG-PET/CT on Clinical Management Decisions in Breast Cancer Patients—A PET/CT Registry Study
Authors: Werner Sebastian , Sekler Julia , Gückel Brigitte , la Fougère Christian , Nikolaou Konstantin , Pfannenberg Christina , Preibsch Heike , Engler Tobias , Olthof Susann-Cathrin
Published: July 2023

There is a lack of evidence regarding the clinical impact of [18F]fluorodeoxyglucose positron emission tomography/computed tomography ([18F]FDG-PET/CT, hereinafter referred to as PET/CT), especially regarding management changes and their link to overall survival. We analyzed 52 PET/CTs in 47 stage I-IV breast cancer patients, selected from a prospective oncological PET/CT registry. Indications for PET/CT were primary staging (n = 15), restaging (n = 17), and suspected recurrence (n = 20). PET/CT-induced management changes were categorized as major or minor. PET/CT-induced management changes in 41 of 52 scans (78.8%; 38 of 47 patients (80.9%)), of which major changes were suggested in 18 of 52 scans (34.6%, 17 of 47 patients, 36.2%). PET/CT downstaged 6 of 15 primary staging patients, excluding distant metastases. Major management changes were documented in 3 of 17 restaging exams. PET/CT ruled out clinically suspected recurrence in 6 of 20 cases and confirmed it in 11 of 20. In three cases, locoregional recurrence had already been diagnosed via biopsy. In 30 of 52 exams, additional diagnostic tests were avoided, of which 13 were invasive. PET/CT-based management changes resulted in a 5-year survival rate of 72.3% for the whole study group, 93.3% for the staging group, 53.8% for the restaging group, and 68.4% for the recurrence group. This study shows that PET/CT significantly impacts clinical management decisions in breast cancer patients in different clinical scenarios, potentially determining the patient’s tumor stage as the basis for further therapy more reliably and by avoiding unnecessary diagnostic tests.
Intracranial Hemorrhage in Hospitalized Patients Following Percutaneous Coronary Intervention: A Large Cohort Analysis from a Single Center
Authors: Yang Cheng , Sui Yong-Gang , Wang Bin-Cheng , Xu Yan-Lu , Wu Na-Qiong , Wu Yong-Jian , Li Jian-Jun , Qian Jie
Published: July 2023

Background: There are several reports on the prevalence and characteristics of intracranial hemorrhage (ICH) following percutaneous coronary intervention (PCI), which is a rare but severe complication with high mortality. However, the clinical landscapes of computed tomography (CT)-confirmed, symptomatic ICH in hospitalized patients are not fully characterized. Methods: Among 121,066 patients receiving PCI treatment in the Fu Wai Hospital between 2013 and 2022, there were 18 CT-defined, symptomatic patients with ICH occurring during post-PCI hospitalization. Symptomatic ICH was defined as clinical suspicion of hemorrhage and/or new focal neurological signs. We analyzed ICH timing, clinical and imaging features, and subsequent outcomes. Results: Overall, in this retrospective analysis, the incidence of CT-defined, symptomatic ICH was 0.015% (18/121,066). More than half of the cases (55.6%) occurred within the first 12 h following PCI. The most common initial manifestation of ICH patients was disturbance of consciousness. Thirteen patients (72.2%) had a hematoma volume ≥ 30 cm3. Additionally, the ICH was observed in the cerebral lobe (66.7%), cerebellum (22.2%), and the basal ganglia and thalamus (11.1%). The 90-day mortality of ICH patients undergoing PCI was very high (72.2%). Consciousness disturbance (p = 0.036), intracerebral hemorrhage volume > 30 mm3 (p = 0.001), and intracerebral hemorrhage originating from the infratentorial origin (p = 0.044) were significantly higher in patients who died. Conclusions: Symptomatic ICH events occur with a rate of around 0.015%, with significantly higher short-term mortality risk in our cohort receiving PCI, which has not yet been demonstrated in other cohorts.
Copper Deficiency as Wilson’s Disease Overtreatment: A Systematic Review
Authors: Litwin Tomasz , Antos Agnieszka , Bembenek Jan , Przybyłkowski Adam , Kurkowska-Jastrzębska Iwona , Skowrońska Marta , Członkowska Anna
Published: July 2023

Background: Treatment of Wilson’s disease (WD), an inherited disease characterized by copper overload, is lifelong and there is the possibility that copper deficiency (CD) may occur. We systematically reviewed the literature to describe treatment patterns, symptoms and outcomes associated with CD. Methods: Using preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, the PubMed database was searched up to 6 April 2023. Results: Across 17 articles, 20 cases of CD were described, most commonly (15 cases) in WD patients treated with zinc salts (ZS), less often on combined chelator and ZS therapy (3 cases), molybdate salts plus ZS (1), or molybdate alone (1). CD symptoms occurred insidiously, including sideroblastic anemia, neutropenia, axonal sensory neuropathy, posterior cord myelopathy and increased ratio of epileptic seizures (or epilepsy). CD diagnosis was based on symptoms and severely reduced urinary copper excretion (<20 µg/24 h [<0.3 µmol/24 h] on ZS, or <100 µg/24 h [<1.6 µmol/24 h] on chelators) with low total serum copper and ceruloplasmin. Conclusions: Awareness of CD and regular monitoring of copper metabolism is needed during WD treatment. Temporary cessation of anti-copper treatment usually reverses serum copper reductions as well as pancytopenia; however, some symptoms, especially neuropathy and myelopathy, may persist.
The Pathophysiology of Collateral Circulation in Acute Ischemic Stroke
Authors: Mangiardi Marilena , Bonura Adriano , Iaccarino Gianmarco , Alessiani Michele , Bravi Maria Cristina , Crupi Domenica , Pezzella Francesca Romana , Fabiano Sebastiano , Pampana Enrico , Stilo Francesco , Alfano Guido , Anticoli Sabrina
Published: July 2023

Cerebral collateral circulation is a network of blood vessels which stabilizes blood flow and maintains cerebral perfusion whenever the main arteries fail to provide an adequate blood supply, as happens in ischemic stroke. These arterial networks are able to divert blood flow to hypoperfused cerebral areas. The extent of the collateral circulation determines the volume of the salvageable tissue, the so-called “penumbra”. Clinically, this is associated with greater efficacy of reperfusion therapies (thrombolysis and thrombectomy) in terms of better short- and long-term functional outcomes, lower incidence of hemorrhagic transformation and of malignant oedema, and smaller cerebral infarctions. Recent advancements in brain imaging techniques (CT and MRI) allow us to study these anastomotic networks in detail and increase the likelihood of making effective therapeutic choices. In this narrative review we will investigate the pathophysiology, the clinical aspects, and the possible diagnostic and therapeutic role of collateral circulation in acute ischemic stroke.
Clinical Impact of the BIOFIRE Blood Culture Identification 2 Panel in Adult Patients with Bloodstream Infection: A Multicentre Observational Study in the United Arab Emirates
Authors: Senok Abiola , Dabal Laila Al , Alfaresi Mubarak , Habous Maya , Celiloglu Handan , Bashiri Safia , Almaazmi Naama , Ahmed Hassan , Mohmed Ayman A. , Bahaaldin Omar , Elimam Maimona Ahmed Elsiddig , Rizvi Irfan Hussain , Olowoyeye Victory , Powell Michaela , Salama Basel
Published: July 2023

Rapid pathogen identification is key to the proper management of patients with bloodstream infections (BSIs), especially in the intensive care setting. This multicentre study compared the time to pathogen identification results in 185 patients admitted to intensive care with a confirmed BSI, using conventional methods (n = 99 patients) and upon implementation of the BIOFIRE® Blood Culture Identification 2 (BCID2) Panel, a rapid molecular test allowing for the simultaneous identification of 43 BSI-related nucleic acids targets (n = 86 patients). The median time to result informing optimal antibiotic therapy was significantly shorter following the implementation of the BCID2 Panel (92 vs. 28 h pre vs. post BCID2 implementation; p < 0.0001). BCID2 usage in addition to conventional methods led to the identification of at least one pathogen in 98.8% patients vs. 87.9% using conventional methods alone (p = 0.003) and was associated with a lower 30-day mortality (17.3% vs. 31.6%, respectively; p = 0.019). This study at three intensive care units in the United Arab Emirates therefore demonstrates that, in addition to conventional microbiological methods and an effective antimicrobial stewardship program, the BCID2 Panel could improve the clinical outcome of patients admitted to the intensive care unit with a confirmed BSI.
Analysis and Precision of Light Detection and Ranging Sensors Integrated in Mobile Phones as a Framework for Registration of Ground Control Points for Unmanned Aerial Vehicles in the Scanning Technique for Building Information Modelling in Archaeological Sites
Authors: Moyano Juan , Nieto-Julián Juan , Fernández-Alconchel María , Oreni Daniela , Estévez-Pardal Rafael
Published: July 2023

The protection of heritage sites is one of the keys that our civilisation presents. That is why great efforts have been invested in order to protect and preserve movable and immovable property with a certain historical value, as is the case of archaeological sites scattered throughout the territory of southern Iberia (Spain) in the form of dolmens and negative structures dug into the ground, constituting a good sample of the megalithic culture in southern Spain. To study, manage and preserve these archaeological monuments, considered a set of cultural assets, various techniques and methodologies are required to facilitate the acquisition of three-dimensional geometric information. The Scan-to-BIM approach has become one of the most up-to-date work exponents to carry out these objectives. The appearance of LiDAR techniques, and recently their incorporation into smartphones through integrated sensors, is revolutionising the world of 3D scanning. However, the precision of these techniques is an issue that has yet to be addressed in the scientific community. That is why this research proposes a framework, through experimental measurement, comparison and knowledge of the limitations of this technology, to know the precision of the use of these smartphones, specifically the iPhone 13 Pro, as a measurement element to establish points of control with the aid of photogrammetry by unmanned aerial vehicles (UAVs) in archaeological sites. The results demonstrate a residual uncertainty of ±5 mm in the capture of GCPs from the mobile phone’s LiDAR light detection and ranging sensor, and there was a deviation of the measurements in a range between 0 and 28 m of distance between the GCPs of (0.021, 0.069) m.
Non-Equilibrium Thermodynamics of Heat Transport in Superlattices, Graded Systems, and Thermal Metamaterials with Defects
Authors: Jou David , Restuccia Liliana
Published: July 2023

In this review, we discuss a nonequilibrium thermodynamic theory for heat transport in superlattices, graded systems, and thermal metamaterials with defects. The aim is to provide researchers in nonequilibrium thermodynamics as well as material scientists with a framework to consider in a systematic way several nonequilibrium questions about current developments, which are fostering new aims in heat transport, and the techniques for achieving them, for instance, defect engineering, dislocation engineering, stress engineering, phonon engineering, and nanoengineering. We also suggest some new applications in the particular case of mobile defects.