bluuebunny/crossref_metadata_2025 huggingface dataset:
Panic Disorder with Agoraphobia in Post-Trauma Epilepsy Patient: a Case Report
Authors: Fitriani Fatimah , Ami Putra Gumilar Fardhani , Syulthoni Zain Budi
Published: May 2024

Epilepsy is a significant health problem in society. Epilepsy prominently affects a person's quality of life, including social and psychological aspects, with the community’s negative attitude and stigmatization also contributing to the decline of quality of life. Psychiatric comorbidities such as anxiety and mood disorders are found to be the most common comorbidities in epilepsy. We reported a 31-year-old male with anxiety, palpitations, headache, and nausea. These complaints were often felt by the patient in a crowded place and his wife did not accompany him. These complaints have been felt by the patient since his father died in 2014. He also had a history of seizures after an accident in 2008, although the seizures had been decreased to 3 to 4 times a month. The patient always worried that the seizure would recur. Examinations showed severe anxiety and moderate depression. EEG showed functional disturbances with epileptogenic potential. CT scan showed gliosis and temporal bone defect. The patient was diagnosed with post-trauma epilepsy and agoraphobia. The patient was treated with antiseizure, sleeping pills, antidepressants, cognitive behavioral therapy, psychotherapy, relaxation therapy, and family psychoeducation. 1 week after treatment, the headache was significantly reduced, and the patient reported sleeping better. This report showed the importance of assessing and treating psychiatric comorbidities in patients with epilepsy to improve their quality of life.
Il ladino
Authors: Colcuc Beatrice
Published: May 2024

The aim of this contribution is a discussion of the state of the Ladin language in the Dolomite region of northern Italy. It outlines the main events of the past in the area, examines the different language types within the territory and highlights the common linguistic aspects as well as the cultural and identity differences. The article explores the use of Ladin in various sociolinguistic contexts and the efforts to standardise its spelling. It also discusses the tools, institutions, and initiatives involved in promoting the use of Ladin.
Le varietà walser dell’Italia settentrionale: Gressoney e il titsch
Authors: Saracco Caterina
Published: May 2024

This contribution aims at illustrating the history of the Walser community of Gressoney (Aosta, Italy), where a language belonging to the dialectal space of the Highest Alemannic is still spoken today. The Titsch language, unfortunately, is not only undergoing the influence of the surrounding Romance languages, but its speakers are also disappearing, and a generational change is currently lacking. Secondly, a first scientific description of the phonetic and phonological system of this variety is provided, as well as some peculiar morphosyntactic and lexical characteristics. Finally, some of the most important projects that the scientific community is carrying out for the preservation and valorization of the Titsch language are described.
Content Validity Testing of the Community Life Engagement Guideposts Fidelity Scale
Authors: Lyons Oliver , Sulewski Jennifer , Kwan Ngai
Published: May 2024

The current study sought to address the lack of tools for defining and measuring the quality of day services and supports through the development of a statistically valid tool—the Community Life Engagement (CLE) Guideposts Fidelity Scale (GFS). A Delphi panel composed of 27 experts in the field of CLE reviewed and validated statements on the GFS. This review resulted in a 72-statement scale. All 72 statements demonstrate content validity ratio of .5 or higher. Findings showed that the panel eliminated statements that took focus away from the individual, statements that promoted specific practices, and statements that relied too heavily on the job-readiness aspects of CLE. Implications for the field as well as further testing and refinement of the GFS are also discussed.
Hand in Hand: Interconnected Narrative and Illustration in Alice's Adventures in Wonderland
Authors: Herndon Karlie
Published: May 2024

Abstract: This essay examines the combination of hands and clothing (or lack thereof) on animals in Sir John Tenniel's illustrations of Alice's Adventures in Wonderland . The hierarchical distinctions of characters in the narrative echo, distort, and question Victorian social classifications and mark Alice—a child—as distinct.
"i thought maby you might help as you other Little Children": Using Letters to Mrs. Roosevelt to Reread the Classed Other in Depression-Era Children's Literature
Authors: Davis Maggie E. Morris
Published: May 2024

Abstract: This essay argues that the language within letters young people wrote to Eleanor Roosevelt offers a standard by which to consider how the voices of fictional children in poverty in Depression-era children's literature can provide nuanced entry points or, at times, misleading impressions of how children linguistically represent poverty.
Generation of sub-150 fs, 10 W-class, pedestal-free 2.07 µm ultrashort pulses using a cascade compression system
Authors: Yang Linjing , Yao Chuanfei , Wang Xuan , Xu Zipeng , Ren Guochuan , Li Kaihang , Li Pingxue
Published: May 2024

We report high-power pedestal-free ultrashort pulses in a cascade compression system. In the self-compression stage, the 2 µm ultrashort pulses with 123 fs duration and up to 21.7 W output power were obtained in a 0.3 m 50 µm core diameter fiber. It is the highest self-compressing power ever obtained in a silica fiber with an all-fiber 2 µm laser amplifier as the pump source. To obtain purer pedestal-eliminated pulses, we further increase the fiber length to 1 m to trigger the soliton self-frequency shifting (SSFS) effect. By employing an enhanced SSFS technique based on third-order dispersion (TOD) and filtering out the unshifted signal light, we finally achieved sub-150 fs, 10 W-class, 1.2-MW peak-power, pedestal-free 2.07 µm ultrashort pulses. This is also the highest, to the best of our knowledge, power and energy of Raman soliton obtained by SSFS in an ordinary silica fiber.
Disentangling the effects of metabolic cost and accuracy on movement speed
Authors: Bruening Garrick W. , Courter Robert J. , Sukumar Shruthi , O’Brien Megan K. , Ahmed Alaa A.
Published: May 2024

On any given day, we make countless reaching movements to objects around us. While such ubiquity may suggest uniformity, each movement’s speed is unique—why is this? Reach speed is known to be influenced by accuracy; we slow down to sustain high accuracy. However, in other forms of movement like walking or running, metabolic cost is often the primary determinant of movement speed. Here we bridge this gap and ask: how do metabolic cost and accuracy interact to determine speed of reaching movements? First, we systematically measure the effect of increasing mass on the metabolic cost of reaching across a range of movement speeds. Next, in a sequence of three experiments, we examine how added mass affects preferred reaching speed across changing accuracy requirements. We find that, while added mass consistently increases metabolic cost thereby leading to slower metabolically optimal movement speeds, self-selected reach speeds are slower than those predicted by an optimization of metabolic cost alone. We then demonstrate how a single model that considers both accuracy and metabolic costs can explain preferred movement speeds. Together, our findings provide a unifying framework to illuminate the combined effects of metabolic cost and accuracy on movement speed and highlight the integral role metabolic cost plays in determining reach speed.
COVID-19 contact tracing and quarantine policies in the Indo-Pacific Region: A mixed-methods study of experiences of public health professionals
Authors: Islam Md. Saiful , Vogt Florian , King Catherine , Sheel Meru
Published: May 2024

Contact tracing and quarantine are valuable public health tools to prevent the transmission of SARS-CoV-2 and control the epidemic. Many low-and middle-income countries (LMICs) adopted global contact tracing and quarantine guidelines but were unable to contextualise the guidance into policies and practices that were relevant to their setting. Therefore, we examine contact tracing policies and practices in the Indo-Pacific region and the need to design context-specific policies. We conducted a mixed-methods study, including a cross-sectional online survey followed by key-informant interviews (KIIs). Using convenience snowball sampling, we invited public health professionals primarily involved in COVID-19 pandemic response from the Indo-Pacific region. We undertook descriptive analyses using counts and percentages for survey data and framework analysis for qualitative data. Seventy-seven public health professionals participated in the survey, of whom ten also participated in the KIIs. The study identified significant gaps between policies and the local contexts. Factors that broaden the gaps were limited knowledge of the changing dynamics of COVID-19 transmission, poor leadership, and coordination, little or no formal training on contact tracing, poor understanding of the guideline recommendations, limited resources, community resistance and mistrust, social stigmatisation and fear of being ostracised, and discrimination. This study revealed substantial disparities between policies and local contexts, significantly influencing policy implementation at national, provincial, and district levels across the studied countries. To bridge these gaps, we advocate for national contact tracing and quarantine guidelines explicitly addressing the quarantine needs of specific demographics, including children, pregnant women, prisoners, and individuals affected by social exclusion issues. Furthermore, we propose strengthening contact tracing training programs, urging revised guidelines to account for social, cultural, and infrastructural nuances influencing contact tracing and quarantine implementation. We also recommend engaging local NGOs, faith-based organisations, and local administrations to reinforce community connections and strengthen contact tracing.
A panel of phenotypically and genotypically diverse bioluminescent:fluorescent Trypanosoma cruzi strains as a resource for Chagas disease research
Authors: Olmo Francisco , Jayawardhana Shiromani , Khan Archie A. , Langston Harry C. , Francisco Amanda Fortes , Atherton Richard L. , Ward Alex I. , Taylor Martin C. , Kelly John M. , Lewis Michael D.
Published: May 2024

Chagas disease is caused by Trypanosoma cruzi, a protozoan parasite that displays considerable genetic diversity. Infections result in a range of pathological outcomes, and different strains can exhibit a wide spectrum of anti-parasitic drug tolerance. The genetic determinants of infectivity, virulence and therapeutic susceptibility remain largely unknown. As experimental tools to address these issues, we have generated a panel of bioluminescent:fluorescent parasite strains that cover the diversity of the T. cruzi species. These reporters allow spatio-temporal infection dynamics in murine models to be monitored in a non-invasive manner by in vivo imaging, provide a capability to detect rare infection foci at single-cell resolution, and represent a valuable resource for investigating virulence and host:parasite interactions at a mechanistic level. Importantly, these parasite reporter strains can also contribute to the Chagas disease drug screening cascade by ensuring that candidate compounds have pan-species in vivo activity prior to being advanced into clinical testing. The parasite strains described in this paper are available on request.
Association between body mass index and prevalence of bacterial vaginosis: Results from the NHANES 2001–2004 study
Authors: Qi Jie , Han Hua , Li Xinjun , Ren Yanan
Published: May 2024

Background

The impact of bacterial vaginosis on women’s health is an increasing concern; however, the effect of the obesity index on bacterial vaginosis is controversial. We investigated the association between body mass index and bacterial vaginosis in women in the United States.

Methods

This was a cross-sectional study which obtained the data from the National Health and Nutrition Examination Survey from 2001 to 2004, in which weighted multivariate regression and logistic regression analyses were performed to explore the independent relationship between body mass index and bacterial vaginosis. Subgroup analyses and smoothed curve fitting were also performed.

Results

A total of 5,428 participants were enrolled, and the findings show that the participants with higher body mass index tended to have a higher incidence of bacterial vaginosis. In the fully adjusted model, a positive association between bacterial vaginosis and body mass index was observed (Odd’s ratio = 1.03, 95% Confidence interval, 1.01–1.04). The subgroup analysis showed that this positive association was significant in non-Hispanic White individuals (Odd’s ratio = 1.0327, 95% Confidence interval, 1.0163, 1.0493).

Conclusion

Increased bacterial vaginosis positivity may be associated with an increased body mass index.
Smart transition pathways and development incentive mechanism of China’s smart community elderly care industry under market dominance: Considering a multi-subjective behavior game
Authors: Mao Qinghua , Mao Yining , Sun Qilong , Xu Linyao
Published: May 2024

Against the backdrop of an aging population, China is actively experimenting with an innovative elderly care model, so smart community elderly care has recently received widespread attention. However, the results of the implementation of the model have not yet met the expectation due to the variety of interests among the relevant participants. In this study, we identified the most core stakeholders in smart community elderly care, developed a four-party evolutionary game model including local governments, communities, service supply enterprises and households with elderly members. By applying the system dynamics method, we simulate the evolutionary paths and explore the complex interactions at the multiparticipant level in order to facilitate the transition of community elderly care services from traditional to smart, and then propose managerial insights for accelerating the construction of smart community elderly care. The results suggest that: (1) the four players in the game influence each other and are intimately related, and the benign interaction between them will further stimulate the vitality of the smart community elderly care industry; (2) appropriate improvement in policy support will strongly promote smart community elderly care, and the incentive effect on the demand side (households with elderly members) is more significant; (3) when households’ preference for smart services increases, and the perceived value to communities and enterprises reaches a certain threshold, communities and enterprises will actively adopt smart solution and technology as well as develop stable portfolio strategy; (4) measures such as simultaneously increasing the level of smart and resource synergy will promote the system evolution toward smart services, and the system is more sensitive to the internal behavior of the enterprise than the external behavior between community and enterprise.
Mediating roles of perceived stigma and mental health literacy in the relationship between school climate and help-seeking behavior in Indonesian adolescents
Authors: Setia Lesmana Mohammad Hendra , Chung Min-Huey
Published: May 2024

Objective

This study aimed to investigate whether perceived stigma and mental health literacy play mediating roles in the correlation between school climate and help-seeking behavior in Indonesian adolescents.

Methods

We used cross-sectional study design that recruited 760 Indonesian adolescents of age 16–19 years. We used convenience sampling from July to September 2019. Bivariate analysis was used to investigate the association of demographic characteristics with help-seeking behavior. Mediation analysis was employed to explore the mediating roles of mental health literacy and perceived stigma on the relationship between school climate and help-seeking behavior.

Results

Findings indicated that ethnicity, family income, and father’s educational level were significantly associated (p < 0.05) with help-seeking behavior in Indonesian adolescents. Furthermore, perceived stigma and mental health literacy sequentially showed partial mediating roles in the relationship between school climate and help-seeking behavior (indirect effect: 0.004; 95% CI: 0.001, 0.010). Our mediating model indicated that a high level of school climate was associated with low perceived stigma (b = −0.11, p < 0.001) and high mental health literacy (b = 0.28, p < 0.001) and higher help-seeking behavior (b = 0.14, p < 0.001).

Conclusion

Our study discovered novel insight of help-seeking behavior mechanism among adolescent by serial mediation test. Supportive school climate is necessary to achieve adequate help-seeking behavior. In addition, taking into account of student’s perceived stigma and mental health literacy in promoting help-seeking behavior is also important.
Assessing the influence of the health system on access to cervical cancer prevention, screening, and treatment services at public health centers in Addis Ababa, Ethiopia
Authors: Hussein Kemal , Kokwaro Gilbert , Wafula Francis , Kassie Getnet Mitike
Published: May 2024

Background

Cervical cancer is the second leading cause of cancer death among Ethiopian women. This study aimed to assess the influence of the health system on access to cervical cancer prevention, screening, and treatment services at public health centers in Addis Ababa, Ethiopia.

Methods

This study used a cross-sectional survey design and collected data from 51 randomly selected public health centers in Addis Ababa. Open Data Kit was used to administer a semi-structured questionnaire on Android tablets, and SPSS version 26 was used to analyze the descriptive data.

Results

In the study conducted at 51 health centers, cervical cancer prevention and control services achieved 61% HPV vaccination for girls, 79% for cervical cancer awareness messages, 80% for precancer lesion treatment, and 71% for cervical screening of women. All health centers were performing cervical screening mostly through visual inspection with acetic acid due to the inconsistent availability of HPV DNA tests and the lack of Pap smear tests. In 94% of health centers, adequate human resources were available. However, only 78% of nurses, 75% of midwives, 35% of health officers, and 49% of health extension workers received cervical cancer training in the 24 months preceding the study. Women had provider choices in only 65% of health centers, and 86% of the centers lacked electronic health records. In 41% of the health centers, the waiting time was 30 minutes or longer. About 88% and 90% of the facilities lacked audio and video cervical cancer messages, respectively.

Conclusion

This study revealed that the annual cervical cancer screening achievement was on track to fulfill the WHO’s 90-70-90 targets by 2030. We recommend that decision-makers prioritize increasing HPV vaccination rates, enhancing messaging, reducing wait times, and implementing electronic health records to improve access to cervical cancer services in Addis Ababa.
Mapping the distribution of Lyme disease at a mid-Atlantic site in the United States using electronic health data
Authors: Lantos Paul M. , Janko Mark , Nigrovic Lise E. , Ruffin Felicia , Kobayashi Takaaki , Higgins Yvonne , Auwaerter Paul G.
Published: May 2024

Lyme disease is a spatially heterogeneous tick-borne infection, with approximately 85% of US cases concentrated in the mid-Atlantic and northeastern states. Surveillance for Lyme disease and its causative agent, including public health case reporting and entomologic surveillance, is necessary to understand its endemic range, but currently used case detection methods have limitations. To evaluate an alternative approach to Lyme disease surveillance, we have performed a geospatial analysis of Lyme disease cases from the Johns Hopkins Health System in Maryland. We used two sources of cases: a) individuals with both a positive test for Lyme disease and a contemporaneous diagnostic code consistent with a Lyme disease-related syndrome; and b) individuals referred for a Lyme disease evaluation who were adjudicated to have Lyme disease. Controls were individuals from the referral cohort judged not to have Lyme disease. Residential address data were available for all cases and controls. We used a hierarchical Bayesian model with a smoothing function for a coordinate location to evaluate the probability of Lyme disease within 100 km of Johns Hopkins Hospital. We found that the probability of Lyme disease was greatest in the north and west of Baltimore, and the local probability that a subject would have Lyme disease varied by as much as 30-fold. Adjustment for demographic and ecological variables partially attenuated the spatial gradient. Our study supports the suitability of electronic medical record data for the retrospective surveillance of Lyme disease.
The HR revolution: Redefining performance paradigms in Pakistan’s pharma landscape through moderating role of innovative climate
Authors: Waheed Abdul , Waheed Salma , Hussain Shahbaz , Majeed Abdul
Published: May 2024

The main objective of this research is to examine whether the implementation of new human resource management practices (NHRM) can enhance innovative performance (IP) by fostering innovation capability (NC). Additionally, it examines the moderating influence of an innovative climate (IC) and its associated attributes on the association with new human resource management and innovation capability, a factor that has been relatively overlooked in prior research. The study’s data was obtained from 398 employees in the pharmaceutical sector of Pakistan, and hierarchical regression analysis was utilized to analyze the data. The results from the mediating and moderating analyses underscore the significance of NHRM practices, innovation capability, and an innovative climate, along with its specific attributes, in promoting innovative performance through factors such as operational efficiency, suitable practices, and employees’ willingness to participate in organizational endeavors. Furthermore, the moderated mediation analysis findings reveal that the influence of innovation capability as a mediator is strengthened when the workplace climate is more conducive to innovation. These findings have implications for both theoretical understanding and practical application, particularly in similar developing countries. The study offers insights that can be generalized to developing nations with comparable economic and social structures.
Mapping automatic social media information disorder. The role of bots and AI in spreading misleading information in society
Authors: Tomassi Andrea , Falegnami Andrea , Romano Elpidio
Published: May 2024

This paper presents an analysis on information disorder in social media platforms. The study employed methods such as Natural Language Processing, Topic Modeling, and Knowledge Graph building to gain new insights into the phenomenon of fake news and its impact on critical thinking and knowledge management. The analysis focused on four research questions: 1) the distribution of misinformation, disinformation, and malinformation across different platforms; 2) recurring themes in fake news and their visibility; 3) the role of artificial intelligence as an authoritative and/or spreader agent; and 4) strategies for combating information disorder. The role of AI was highlighted, both as a tool for fact-checking and building truthiness identification bots, and as a potential amplifier of false narratives. Strategies proposed for combating information disorder include improving digital literacy skills and promoting critical thinking among social media users.
Poisoning cases and their management in Amhara National Regional State, Ethiopia: Hospital-based prospective study
Authors: Asrie Assefa Belay , Atnafie Seyfe Asrade , Getahun Kefyalew Ayalew , Birru Eshetie Melese , Mekonnen Gashaw Binega , Alemayehu Geta Asrade , Endehabtu Berhanu Fikadie , Badi Marta Berta , Adinew Getinet Mequanint
Published: May 2024

Background

Poisoning is a significant public health problem globally. Ethiopia is a low-income country undergoing technological and social change that may increase access to drugs and chemicals, potentially increasing the incidence of poisoning. This study describes the epidemiology of hospital admissions due to poisoning in a region of Ethiopia.

Methods

An institution based prospective observational study was employed, as a study design, in selected hospitals of the region from January to December 2018.

Results

Of 442 poisoning cases, 78 (17.6%) died. Almost all poisoning cases were intentional self-poisonings. The most frequent poisonings were organophosphate compounds, 145 (32.8%), and metal phosphides (majorly aluminum phosphide), 115 (26.0%). The ingested poison was most frequently accessed from the patients’ homes, 243 (55.0%), followed by purchases from local shops, 159 (36%). The median duration of admission was 24 hours. Of all the cases, 23 (5.2%) were admitted to intensive care units (ICU) requiring mechanical ventilation. Most of the cases admitted to the ICU were aluminum phosphide-poisoned patients. The majority of deaths (43 of 78) were due to metal phosphides. From the multivariate logistic regression analysis, altered level of consciousness on hospital arrival, metal phosphide poisoning, and no laboratory result as a part of the diagnosis process or investigation of the extent of toxicity were found to be significantly associated with the likelihood of poor treatment outcome.

Conclusion

The majority of the poisoning cases were females. The most common reasons for the intent of self-poisoning were dispute-related, mainly family disharmonies, followed by psychiatric conditions. The poisoning agents were mostly obtained from households. Organophosphate compounds and metal phosphides were the first and the second most frequently encountered poisoning agents, respectively, and it was noted that the later ones were responsible for most of the fatal cases. Of the pharmacologic interventions, atropine was the only agent regarded as an antidote. The most commonly employed agent for supportive treatment was cimetidine followed by maintenance fluids, while gastric lavage was the only GI decontamination method used among others. The fatality rate of poisoning in this study was found to be much higher than in other similar studies. Impaired consciousness upon hospital arrival, metal phosphide poisoning, and no involvement of laboratory investigation were found to significantly associate with the likelihood of death. Generally, the results dictate the need for the design and implementation of strategies to create awareness, prevent, and manage poisoning incidences in the community.
Documenting the implementation processes and effects of the data use initiatives in primary health care settings in Tanzania: A before-after mixed methods study protocol
Authors: Kalolo Albino , Kesale Anosisye M. , Anasel Mackfallen , Kapologwe Ntuli A. , Jaribu Jennie , Mujaya Stella , Kengia James T.
Published: May 2024

Background

The use of data in decision making and planning in primary health care settings is critical for improving efficiency and health outcomes for patients and communities. Implementation research can be used to fully understand the effects, context, challenges, and facilitators of data use, as well as how to scale up data use interventions. However, in the context of low resource settings, little is known about how implementation research can be employed to assess the implementation and impact of data use interventions.

Methods

We will conduct a hybrid type 2 effectiveness-implementation study employing a mixed method controlled before and after design to measure the effects of data use interventions while simultaneously understanding the implementation of those initiatives. The controlled before and after entails measurement of the effects of the interventions at baseline and end line in a matched intervention and control health facilities using structured questionnaire to health workers (n = 440) and existing patients (n = 422) while also extracting selected health outcome variable from routine data in all participating health facilities (n = 80). The mixed methods component entails measuring the implementation outcomes (adoption, acceptability, fidelity and maintenance) and their moderators entails the integration of both quantitative and qualitative data collection, analysis, and interpretation (i.e. mixed methods) approach by using a structured questionnaire to implementers (health workers and managers) (n = 400). Experiential dimensions of implementation processes and moderators will be explored using qualitative interviews. Guided by implementation research theories and frameworks, a theory of change (TOC) is developed first to guide the evaluation of implementation processes and effects of the interventions. Descriptive and inferential statistics will be employed to analyze quantitative data whereas thematic analysis approach will be employed for qualitative data.

Discussion

This study is one of the first to test the simultaneous measurement of effects and implementation processes of data use interventions in the primary health care settings. Findings will support efforts to improve quality of services by optimizing scale up and sustainability of the data use initiatives in primary health care settings.
IGF2BP3 regulates the expression of RRM2 and promotes the progression of rheumatoid arthritis via RRM2/Akt/MMP-9 pathway
Authors: Ban Zhaonan , Li Zhengjiang , Xing Shuxing , Ye Yongjie
Published: May 2024

Background

Rheumatoid arthritis (RA) is a common inflammatory and autoimmune disease. Ribonucleotide Reductase Regulatory Subunit M2 (RRM2) is a crucial and a rate-limiting enzyme responsible for deoxynucleotide triphosphate(dNTP) production. We have found a high expression level of RRM2 in patients with RA, but the molecular mechanism of its action remains unclear.

Methods

We analyzed the expression of hub genes in RA using GSE77298 datasets downloaded from Gene Expression Omnibus database. RRM2 and insulin-like growth factor-2 messenger ribonucleic acid (mRNA)-binding protein 3 (IGF2BP3) gene knockdown was achieved by infection with lentiviruses. The expression of RRM2, IGF2BP3, matrix metalloproteinase (MMP)-1, and MMP-9 were detected via western blotting assay. Cell viability was detected via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MeRIP-qRT-PCR was performed to test the interaction of IGF2BP3 and RRM2 mRNA via m6A modification. Cell proliferation was determined by clone formation assay. Migration and invasion assays were performed using transwell Boyden chamber.

Results

RRM2 and IGF2BP3 were highly expressed in clinical specimens and tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β-stimulated synovial cells. RRM2 and IGF2BP3 knockdown inhibited the proliferation, migration, and invasion of MH7A cells. The inhibitory effects of IGF2BP3 knockdown were effectively reversed by simultaneously overexpressing RRM2 in MH7A cells. By analyzing N6-methyladenosine (m6A)2Target database, five m6A regulatory target binding sites for IGF2BP3 were identified in RRM2 mRNA, suggesting a direct relationship between IGF2BP3 and RRM2 mRNA. Additionally, in RRM2 small hairpin (sh)RNA lentivirus-infected cells, the levels of phosphorylated Akt and MMP-9 were significantly decreased compared with control shRNA lentivirus-infected cells.

Conclusion

The present study demonstrated that RRM2 promoted the Akt phosphorylation leading to high expression of MMP-9 to promote the migration and invasive capacities of MH7A cells. Overall, IGF2BP promotes the expression of RRM2, and regulates the migration and invasion of MH7A cells via Akt/MMP-9 pathway to promote RA progression.
Influence of COVID-19 on female sex workers in Dar es Salaam, Tanzania: A mixed-methods analysis
Authors: Balampama Marianna , de Walque Damien , Dow William H. , Hémono Rebecca
Published: May 2024

This study investigates how the landscape of sex work in Dar es Salaam, Tanzania, evolved in the context of the COVID-19 epidemic. Using a mixed-methods approach, the analysis triangulates data from quantitative and qualitative sources to quantify shifts in income, demand, and client frequency and describe female sex workers’ perspectives on their work environment. The COVID-19 restrictions introduced in early 2020 resulted in dramatic decreases in sex work income, leading to extreme financial vulnerability, food insecurity, and challenges in meeting other basic needs such as paying rent. However, in a 2021 follow-up survey, sex workers reported the summer of 2021 as a key turning point, with the demand for sex work rebounding to closer to pre-pandemic levels. Notably, despite the average number of unique weekly clients not yet having fully rebounded, by 2021 the price per client and the total monthly sex work income had returned to pre-pandemic levels. This may potentially be explained by an increased number of repeat clients, which represented a larger proportion of all clients during the COVID-19 pandemic.
Development and testing of a questionnaire on the expectations and experiences with wearing face masks in inpatient and day hospitals
Authors: Kuczyk Charlotte , Münch Kathrin , Nöhre Mariel , Stephan Michael , de Zwaan Martina
Published: May 2024

Background

The Covid-19 pandemic made wearing of face masks mandatory in the psychotherapeutic context. Against this background, the present study aimed to compare the expectations of patients undergoing day-hospital or inpatient treatment regarding wearing a mask in psychotherapy before the start of therapy with the final experience after the end of therapy. The study also investigated the extent to which expectations and experiences were influenced by other factors such as socio-demographic characteristics, patients’ general attitudes towards wearing a mask, duration of treatment, or mental health diagnoses.

Methods

Patients’ expectations and experiences were recorded using two versions of a self-developed questionnaire: the pre-version, which was administered before the start of therapy and recorded expectations, and the post-version, which was administered after the end of therapy and recorded the final experiences. An exploratory factor analysis was conducted for the questionnaire’s pre- and post-version. T-tests for paired samples were calculated to compare the patients’ expectations regarding the extracted factors with the final experiences. Bivariate correlations were calculated to explore the association of other potential factors with expectations and experiences.

Results

The exploratory factor analysis revealed a three-factor structure: communication barriers, self-confidence, and infection protection. The communication barriers expected by the patients before the start of the therapy turned out to be significantly higher than ultimately experienced after the therapy. Higher age correlated significantly negatively with expectations and experiences, with less self-confidence expected and experienced in therapy with a mask by older patients. There was a significant positive correlation between the expectations and the duration of treatment. Patients’ general attitudes correlated significantly with their expectations and experiences.

Conclusion

Based on the results, wearing a mask does not appear to negatively impact the success of psychotherapy from the patient’s perspective. However, patient-specific characteristics also appear to play a role in this context.
Confidence, attitude, and practice of scientific research among health professions’ students in the United Arab Emirates
Authors: Jarab Anan S. , Al-Qerem Walid , Alzoubi Karem H. , Abu Heshmeh Shrouq R. , Hayek Mays , Al Hamarneh Yazid N. , Aburuz Salahdein
Published: May 2024

Objective

This study aimed to assess the confidence, attitude, and scientific research practices of undergraduate students of different health profession specialties.

Methods

In this cross-sectional study, an online-based questionnaire was distributed as a Google Form via groups and pages of medical universities available on social media sites such as Facebook, WhatsApp, and Twitter to the second- to sixth-year students of different health profession specialties in different universities across the United Arab Emirates (UAE) in the period from October through December 2023 using the convenience sampling technique. The questionnaire included four parts that assessed socio-demographics and custom-designed research-related questions (6 items), perceived confidence (8 items), attitudes (14 items), and the practice in the context of scientific research and its implementation (9 items). Multivariate logistic regression analyses were conducted to explore the variables associated with the study outcomes, including confidence, attitudes, and practice levels.

Results

The study included 522 undergraduate students. The participants reported low confidence, a negative attitude, and low scientific research practice. Regression results revealed that individuals without prior research experiences were less likely to have high confidence and practice compared to those with previous research experience (OR = 0.634, 95% CI: 0.426–0.945, p = 0.025; and OR = 0.139, 95%Cl: 0.090–0.216, P<0.001, respectively). Additionally, participants who reported difficulty in differentiating between various literature resources were less likely to have high confidence and practice compared to those who reported the ability to differentiate (OR = 0.627, 95% CI: 0.42–0.935, p = 0.022, and OR = 0.370, 95%Cl: 0.237–0.579, p<0.001, respectively). Furthermore, individuals who had not taught research methods in their undergraduate studies were less likely to have high practice (OR = 0.505, 95%Cl: 0.309–0.823, p = 0.06).

Conclusions

Undergraduates of different medical specialties in the UAE demonstrated acceptable levels of confidence and attitude toward scientific research, with several areas for practice improvement. Education and training courses focusing on various aspects of scientific research should be incorporated into the medical curricula in order to enhance students’ confidence and practice of scientific research.
A multicentral prospective cohort trial of a pharmacist-led nutritional intervention on serum potassium levels in outpatients with chronic kidney disease: The MieYaku-Chronic Kidney Disease project
Authors: Asai Yuki , Muramatsu Asami , Kobayashi Tatsuya , Takasaki Ikuhiro , Murasaka Toshiki , Izukawa Ai , Miyada Kahori , Okazaki Takahiro , Yanagawa Tatsuki , Abe Yasuharu , Takai Yasushi , Iwamoto Takuya
Published: May 2024

Although dietary potassium restriction is an acceptable approach to hyperkalemia prevention, it may be insufficient for outpatients with chronic kidney disease (CKD). Most outpatients with CKD use community pharmacies owing to the free access scheme in Japan. The MieYaku-CKD project included a community pharmacist-led nutritional intervention for dietary potassium restriction, with the goal of determining its efficacy for patients’ awareness of potassium restriction and serum potassium levels in outpatients with CKD. This was a five-community pharmacy multicenter prospective cohort study with an open-label, before-and-after comparison design. Eligible patients (n = 25) with an estimated glomerular filtration rate (eGFR) < 45 mL/min/1.73 m2 received nutritional guidance from community pharmacists. The primary outcome was a change in serum potassium levels at 12 weeks post-intervention. The eligible patients’ knowledge, awareness, and implementation of potassium restriction were evaluated using a questionnaire. The median value of serum potassium was significantly reduced from 4.7 mEq/L before to 4.4 mEq/L after the intervention [p < 0.001, 95% confidence interval (CI): 0.156–0.500], with no changes in eGFR (p = 0.563, 95% CI: -2.427–2.555) and blood urine nitrogen/serum creatinine ratio (p = 0.904, 95% CI: -1.793–1.214). The value of serum potassium had a tendency of attenuation from 5.3 to 4.6 mEq/L (p = 0.046, 95% CI: 0.272–1.114) in the eGFR < 30 mL/min/1.73 m2 group. A questionnaire revealed that after the intervention, knowledge and attitudes regarding dietary potassium restriction were much greater than before, suggesting that the decrease in serum potassium levels may be related to this nutritional guidance. Our findings indicate that implementing a dietary potassium restriction guidance program in community pharmacies is feasible and may result in lower serum potassium levels in outpatients with CKD.
The impact of caregiving on the roles and valued activities of stroke carers: A systematic review of qualitative studies
Authors: Jammal Melissa , Kolt Gregory S. , Liu Karen P. Y. , Dennaoui Nariman , George Emma S.
Published: May 2024

Objective

To understand the experiences of informal carers and the impact of role and activity changes on their health and wellbeing.

Methods

A systematic search of CINHAL, MEDLINE, Embase, APA PsycInfo, and Web of Science was conducted. Studies were eligible if they included informal stroke carers (≥18 years), used a qualitative methodology, explored the roles and valued activities of stroke carers, and were published in English. The 10-item Critical Appraisal Skills Programme checklist for qualitative studies was used to assess methodological quality. The results of the included studies were thematically synthesised.

Results

A total of 36 qualitative studies were included and four overarching themes were identified: (1) Life adjustment; (2) Changing role and identity; (3) Changing activities: From meaningful to purposeful; and (4) Understanding and supporting carers.

Conclusion

The sudden nature of stroke requires major readjustment in the carers life that has implications on their relationships, roles, and activities, subsequently impacting on their health and wellbeing. Health professionals and researchers should collaborate with stroke carers to identify their valued activities and implement realistic strategies to maintain these activities. Future interventions designed for carers should implement education about the importance of participating in valued activities and strategies to maintain these activities.
Exploration of underlying patterns among conflict, socioeconomic and political factors
Authors: Vazquez Kathleen , Johnson Jeffrey C. , Griffith David , Muneepeerakul Rachata
Published: May 2024

The emergence of conflict is a complex issue with numerous drivers and interactions playing a role. Exploratory dimension-reduction techniques can reveal patterns of association in such complex data. In this study, an existing dataset was reanalyzed using factor analysis for mixed data to visualize the data in two-dimensional space to explore the conditions associated with high levels of conflict. The first dimension was strongly associated with resilience index, control of corruption, income, income inequality, and regime type, while the second dimension was strongly associated with oil production, regime type, conflict level, political terror level, and water stress. Hierarchical clustering from principal components was used to group the observations into five clusters. Country trajectories through the two-dimensional space provided examples of how movement in the first two dimensions reflected changes in conflict, political terror, regime type, and resilience index. These trajectories correspond to the evolution of themes in research on conflict, particularly in terms of considering the importance of climate or environmental variables in stimulating or sustaining conflict. Understanding conditions associated with high conflict can be helpful in guiding the development of future models for prediction and risk assessment.
Effects of caffeine on temporal perception in Rattus norvegicus
Authors: Keen Richard , Hardy Dalene , Jose Belda , Erturk H. Neval
Published: May 2024

We report two studies that tested the effects of caffeine, the world’s most widely used psychoactive drug, on temporal perception. We trained Wistar rats using the Bisection Procedure (Experiment 1) or the Stubbs’ Procedure (Experiment 2) to discriminate between short and long light stimuli. Once training finished, we administered caffeine orally (0, 9.6, and 96.0 mg/kg for Experiment 1 and 0, 9.6, 19.2, and 38.4 mg/kg for Experiment 2) 15 minutes prior to testing. Relative to the control condition, the 9.6 mg/kg condition (Experiments 1 and 2) and the 19.2 mg/kg condition (Experiment 2) resulted in an increase in proportion of choosing the long response. Meanwhile, overall accuracy was not affected by any condition in both experiments. Taken together, these results are consistent with the notion that caffeine, at some doses, speeds up temporal perception. However, it is not clear why the effect disappears at higher doses.
Circ_0000006 and circ_0000160 regulate hsa-let-7e-5p/UBQLN4 axis in aortic dissection progression
Authors: Liu Yong , Wang Liang , Lei Dongyun , Tan Xiong , Jin Weitao , Hou Ming , Hu Kai , Yan Yu , Wang Hao , Xiang Chaohu , Lai Yinglong
Published: May 2024

Aortic aneurysms (AA) and aorta dissection (AD) are life-threatening conditions with a rising incidence and high mortality rate. Recent research has linked non-coding RNAs to the regulation of AA and AD progression. In this study, we performed circRNA sequencing, microRNA (miRNA) sequencing, and messenger RNA (mRNA) sequencing on plasma samples from AA and AD patients to identify the key circRNA-miRNA-mRNA axis involved in the transition from AA to AD. Our results showed elevated levels of circ_0000006 and circ_0000160, along with decreased levels of hsa-let-7e-5p in AD samples compared to AA samples. Predictive analysis suggested that circ_0000006 and circ_0000160 potentially target hsa-let-7e-5p, which in turn may bind to the mRNA of Ubiquilin 4 (UBQLN4). In an AD cell model using vascular smooth muscle cells (VSMCs), silencing circ_0000006 and circ_0000160 attenuated the effects of platelet-derived growth factor (PDGF)-induced phenotypic changes, proliferation, and migration. This effect was partially reversed by inhibiting hsa-let-7e-5p. Furthermore, we found that overexpression of UBQLN4 counteracted the effects of hsa-let-7e-5p, suggesting UBQLN4 as a downstream mediator of hsa-let-7e-5p. In an animal model of AD, knockdown of circ_0000006 and circ_0000160 also showed protective effects against aortic septation. Overall, our findings indicate that the upregulation of circ_0000006 and circ_0000160 contributes to the progression from AA to AD by influencing abnormal phenotypic changes, migration, and proliferation of VSMCs. The Hsa-let-7e-5p/UBQLN4 axis may play a critical role in AD development. Targeting circ_0000006 and circ_0000160 could be a potential therapeutic strategy for preventing the progression of AD.