bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamics of Mechanical Oscillator Mechanism for Stratospheric Gravity Waves Generated by Convection
Authors: Yu Shiwang , Zhang Lifeng , Zhang Ming , Wang Yuan
Published: September 2020

The mechanical oscillator mechanism (MOM) for stratospheric gravity waves generated by convection is investigated with a dynamics model using the two-dimensional, nonhydrostatic and linear governing equations based on the Boussinesq approximation. The model is solved analytically with a fixed buoyancy oscillation (BO) at the tropopause as the boundary conditions. Results show that this BO is the source of stratospheric gravity waves and the MOM is the generation mechanism. The characteristics of the stratospheric gravity waves not only depend on the BO, but also rely on the stratospheric state, such as the background wind and the buoyancy frequency. When the vertical wavenumbers of the stratospheric gravity waves are close to those of the intrinsic characteristic waves (ICWs), which are the model solution without BO forcing at the tropopause, resonance occurs. Under the resonance conditions, the amplitudes of the stratospheric gravity waves increase significantly, even for low BO intensity. The background wind in the stratosphere has a large effect on wave resonance. Finally, numerical simulation results of a low-vortex system also verify that the MOM is the generation mechanism of stratospheric gravity waves generated by convection.
An AI-Based Automated Continuous Compliance Awareness Framework (CoCAF) for Procurement Auditing
Authors: Wang Ke , Zipperle Michael , Becherer Marius , Gottwalt Florian , Zhang Yu
Published: September 2020

Compliance management for procurement internal auditing has been a major challenge for public sectors due to its tedious period of manual audit history and large-scale paper-based repositories. Many practical issues and potential risks arise during the manual audit process, including a low level of efficiency, accuracy, accountability, high expense and its laborious and time consuming nature. To alleviate these problems, this paper proposes a continuous compliance awareness framework (CoCAF). It is defined as an AI-based automated approach to conduct procurement compliance auditing. CoCAF is used to automatically and timely audit an organisation’s purchases by intelligently understanding compliance policies and extracting the required information from purchasing evidence using text extraction technologies, automatic processing methods and a report rating system. Based on the auditing results, the CoCAF can provide a continuously updated report demonstrating the compliance level of the procurement with statistics and diagrams. The CoCAF is evaluated on a real-life procurement data set, and results show that it can process 500 purchasing pieces of evidence within five minutes and provide 95.6% auditing accuracy, demonstrating its high efficiency, quality and assurance level in procurement internal audit.
The Zebrafish Perivitelline Fluid Provides Maternally-Inherited Defensive Immunity
Authors: De la Paz Javiera F. , Anguita-Salinas Consuelo , Díaz-Celis César , Chávez Francisco P. , Allende Miguel L.
Published: September 2020

In the teleost egg, the embryo is immersed in an extraembryonic fluid that fills the space between the embryo and the chorion and partially isolates it from the external environment, called the perivitelline fluid (PVF). The exact composition of the PVF remains unknown in vertebrate animals. The PVF allows the embryo to avoid dehydration, to maintain a safe osmotic balance and provides mechanical protection; however, its potential defensive properties against bacterial pathogens has not been reported. In this work, we determined the global proteomic profile of PVF in zebrafish eggs and embryos, and the maternal or zygotic origin of the identified proteins was studied. In silico analysis of PVF protein composition revealed an enrichment of protein classes associated with non-specific humoral innate immunity. We found lectins, protease inhibitors, transferrin, and glucosidases present from early embryogenesis until hatching. Finally, in vitro and in vivo experiments done with this fluid demonstrated that the PVF possessed a strong agglutinating capacity on bacterial cells and protected the embryos when challenged with the pathogenic bacteria Edwardsiella tarda. Our results suggest that the PVF is a primitive inherited immune extraembryonic system that protects the embryos from external biological threats prior to hatching.
The Inducible Role of Ambient Particulate Matter in Cancer Progression via Oxidative Stress-Mediated Reactive Oxygen Species Pathways: A Recent Perception
Authors: Lee Chiang-Wen , Vo Thi Thuy Tien , Wu Ching-Zong , Chi Miao-Ching , Lin Chieh-Mo , Fang Mei-Ling , Lee I-Ta
Published: September 2020

Cancer is one of the leading causes of premature death and overall death in the world. On the other hand, fine particulate matter, which is less than 2.5 microns in aerodynamic diameter, is a global health problem due to its small diameter but high toxicity. Accumulating evidence has demonstrated the positive associations between this pollutant with both lung and non-lung cancer processes. However, the underlying mechanisms are yet to be elucidated. The present review summarizes and analyzes the most recent findings on the relationship between fine particulate matter and various types of cancer along with the oxidative stress mechanisms as its possible carcinogenic mechanisms. Also, promising antioxidant therapies against cancer induced by this poison factor are discussed.
Carbonic Anhydrases: Versatile and Useful Biocatalysts in Chemistry and Biochemistry
Authors: Angeli Andrea , Carta Fabrizio , Supuran Claudiu T.
Published: September 2020

Metalloenzymes such as the carbonic anhydrases (CAs, EC 4.2.1.1) possess highly specialized active sites that promote fast reaction rates and high substrate selectivity for the physiologic reaction that they catalyze, hydration of CO2 to bicarbonate and a proton. Among the eight genetic CA macrofamilies, α-CAs possess rather spacious active sites and show catalytic promiscuity, being esterases with many types of esters, but also acting on diverse small molecules such as cyanamide, carbonyl sulfide (COS), CS2, etc. Although artificial CAs have been developed with the intent to efficiently catalyse non-biologically related chemical transformations with high control of stereoselectivity, the activities of these enzymes were much lower when compared to natural CAs. Here, we report an overview on the catalytic activities of α-CAs as well as of enzymes which were mutated or artificially designed by incorporation of transition metal ions. In particular, the distinct catalytic mechanisms of the reductase, oxidase and metatheses-ase such as de novo designed CAs are discussed.
Tandem Synthesis of High Yield MoS2 Nanosheets and Enzyme Peroxidase Mimicking Properties
Authors: Thangudu Suresh , Lee Mu Tzu , Rtimi Sami
Published: September 2020

Molybdenum Sulfide nanosheets (MoS2 NSs) have unique properties that allow its use in a wide range of applications. Unfortunately, a lack of green synthesis methods to achieve a high yield remains a challenge after decades. Herein we report a simple, ecofriendly, green and cost-effective approach to synthesize water soluble MoS2 NSs via probe/Tip sonication method. The sequential batch manner pathway allows us to attain a high yield of MoS2 NSs (~100%). The prepared MoS2 NSs were characterized using up-to-date surface science techniques. UV-visible-NIR spectroscopy allowed us to visualize the doublet peaks of pristine MoS2 at 610 and 680 nm concomitant with the inter-band transitions at 394 nm and 460 nm. Using Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS), the crystallites’ sizes were estimated. X-ray diffraction (XRD) and Raman Spectroscopy were performed with respect to the bulk MoS2. The energy difference between the Raman peaks revealed that our NSs are formed of 5–6 layers. Further, we explored enzyme peroxidase mimetic properties of the synthesized MoS2 NSs. Results showed that the present MoS2 NSs offer excellent peroxidase mimicking properties. Most importantly, we observed that the optical properties and characteristics of MoS2 NSs synthesized by the current green method are similar to those of MoS2 NSs synthesized using conventional harsh methods reported in the literature. So that we strongly assume that the present method is a green alternative for the existing low yield and harsh experimental procedures to achieve water soluble MoS2 NSs in high yield. The synthesized soluble NSs are promising catalysts for the detection of toxic chemicals in the environment and/or for following enzymatic chromogenic reactions.
From Alkynes to Heterocycles through Metal-Promoted Silylformylation and Silylcarbocyclization Reactions
Authors: Albano Gianluigi , Aronica Laura Antonella
Published: September 2020

Oxygen and nitrogen heterocyclic systems are present in a large number of natural and synthetic compounds. In particular, oxa- and aza-silacyclane, tetrahydrofuran, benzofuran, cycloheptadifuranone, cycloheptadipyrrolone, pyrrolidine, lactone, lactam, phthalan, isochromanone, tetrahydroisoquinolinone, benzoindolizidinone, indoline and indolizidine scaffolds are present in many classes of biologically active molecules. Most of these contain a C=O moiety which can be easily introduced using carbonylative reaction conditions. In this field, intramolecular silylformylation and silylcarbocyclization reactions may afford heterocyclic compounds containing a carbonyl functional group together with a vinylsilane moiety which can be further transformed. Considering these two aspects, in this review a detailed analysis of the literature data regarding the application of silylformylation and silylcarbocyclization reactions to the synthesis of several heterocyclic derivatives is reported.
Immobilization of Ir(OH)3 Nanoparticles in Mesospaces of Al-SiO2 Nanoparticles Assembly to Enhance Stability for Photocatalytic Water Oxidation
Authors: Sakamoto Gentaro , Tabe Hiroyasu , Yamada Yusuke
Published: September 2020

Iridium hydroxide (Ir(OH)3) nanoparticles exhibiting high catalytic activity for water oxidation were immobilized inside mesospaces of a silica-nanoparticles assembly (SiO2NPA) to suppress catalytic deactivation due to agglomeration. The Ir(OH)3 nanoparticles immobilized in SiO2NPA (Ir(OH)3/SiO2NPA) catalyzed water oxidation by visible light irradiation of a solution containing persulfate ion (S2O82−) and tris(2,2′-bipyridine)ruthenium(II) ion ([RuII(bpy)3]2+) as a sacrificial electron acceptor and a photosensitizer, respectively. The yield of oxygen (O2) based on the used amount of S2O82− was maintained over 80% for four repetitive runs using Ir(OH)3/SiO2NPA prepared by the co-accumulation method, although the yield decreased for the reaction system using Ir(OH)3/SiO2NPA prepared by the equilibrium adsorption method or Ir(OH)3 nanoparticles without SiO2NPA support under the same reaction conditions. Immobilization of Ir(OH)3 nanoparticles in Al3+-doped SiO2NPA (Al-SiO2NPA) results in further enhancement of the catalytic stability with the yield of more than 95% at the fourth run of the repetitive experiments.
Green Catalysts: Applied and Synthetic Photosynthesis
Authors: Teodor Alexandra H. , Sherman Benjamin D. , Ison Zeah Yvette , Ooi Eu-Jee , Bergkamp Jesse J. , Bruce Barry D.
Published: September 2020

The biological process of photosynthesis was critical in catalyzing the oxygenation of Earth’s atmosphere 2.5 billion years ago, changing the course of development of life on Earth. Recently, the fields of applied and synthetic photosynthesis have utilized the light-driven protein–pigment supercomplexes central to photosynthesis for the photocatalytic production of fuel and other various valuable products. The reaction center Photosystem I is of particular interest in applied photosynthesis due to its high stability post-purification, non-geopolitical limitation, and its ability to generate the greatest reducing power found in nature. These remarkable properties have been harnessed for the photocatalytic production of a number of valuable products in the applied photosynthesis research field. These primarily include photocurrents and molecular hydrogen as fuels. The use of artificial reaction centers to generate substrates and reducing equivalents to drive non-photoactive enzymes for valuable product generation has been a long-standing area of interest in the synthetic photosynthesis research field. In this review, we cover advances in these areas and further speculate synthetic and applied photosynthesis as photocatalysts for the generation of valuable products.
Electrospun Active Media Based on Polyvinylidene Fluoride (PVDF)-Graphene-TiO2 Nanocomposite Materials for Methanol and Acetaldehyde Gas-Phase Abatement
Authors: Boaretti Carlo , Vitiello Giuseppe , Luciani Giuseppina , Lorenzetti Alessandra , Modesti Michele , Roso Martina
Published: September 2020

The abatement of organic pollutants by TiO2 photocatalysis has been established as one of the benchmark applications of advanced oxidation processes for both liquid and gas phase purification. Such solution is particularly suitable for indoor air pollution where volatile organic compounds (VOCs) represent a class of chemicals of high concern for their adverse effects on both environment and human health. However, different shortcomings still affects TiO2 photocatalytic performance in terms of weak adsorptivity and fast electron-hole recombination, limiting its applicability. As a result, different strategies have been investigated over the last years in order to promote a higher TiO2 photo-efficiency. In this study we used electrospun (PVDF) nanofibers as a support for the photo catalytic system obtained by coupling graphene based materials and TiO2 during solvothermal synthesis. The resultant nanostructured membranes have been tested for acetaldehyde and methanol degradation under UV light showing an increase in the photocatalytic activity compared to bare TiO2. Such results may be ascribed to the decrease of band-gap energy and to increased electron mobility in the photocatalytic nanocomposite.
Efficient Broadband Truncated-Pyramid-Based Metamaterial Absorber in the Visible and Near-Infrared Regions
Authors: Dang Phuc Toan , Vu Tuan V. , Kim Jongyoon , Park Jimin , Nguyen Van-Chuc , Vo Dat D. , Nguyen Truong Khang , Le Khai Q. , Lee Ji-Hoon
Published: September 2020

We present a design of an ultra-broadband metamaterial absorber in the visible and near- infrared regions. The unit cell structure consists of a single layer of metallic truncated-pyramid resonator-dielectric-metal configuration, which results in a high absorption over a broad wavelength range. The absorber exhibits 98% absorption at normal incidence spanning a wideband range of 417–1091 nm, with >99% absorption within 822–1054 nm. The broadband absorption stability maintains 95% at large incident angles up to 40° for the transverse electric (TE)-mode and 20° for the transverse magnetic (TM)-mode. Furthermore, the polarization-insensitive broadband absorption is presented in this paper by analyzing absorption performance with various polarization angles. The proposed absorber can be applied for applications such as solar cells, infrared detection, and communication systems thanks to the convenient and compatible bandwidth for electronic THz sources.
Sensitivity, Specificity and Predictive Values of Molecular and Serological Tests for COVID-19: A Longitudinal Study in Emergency Room
Authors: Bisoffi Zeno , Pomari Elena , Deiana Michela , Piubelli Chiara , Ronzoni Niccolò , Beltrame Anna , Bertoli Giulia , Riccardi Niccolò , Perandin Francesca , Formenti Fabio , Gobbi Federico , Buonfrate Dora , Silva Ronaldo
Published: September 2020

Background: We assessed the sensitivity, specificity and positive and negative predictive value (PPV and NPV) of molecular and serological tests for the diagnosis of SARS-CoV-2 infection. Methods: A total of 346 patients were enrolled in the emergency room. We evaluated three Reverse Transcriptase-real time PCRs (RT-PCRs) including six different gene targets, five serologic rapid diagnostic tests (RDT) and one ELISA. The final classification of infected/non-infected patients was performed using Latent Class Analysis combined with clinical re-assessment of incongruous cases. Results: Out of these, 24.6% of patients were classified as infected. The molecular test RQ-SARS-nCoV-2 showed the highest performance with 91.8% sensitivity, 100% specificity, 100.0% PPV and 97.4% NPV respectively. Considering the single gene targets, S and RdRp of RQ-SARS-nCoV-2 had the highest sensitivity (94.1%). The in-house RdRp presented the lowest sensitivity (62.4%). The specificity ranged from 99.2% for in-house RdRp and N2 to 95.0% for E. The PPV ranged from 97.1% of N2 to 85.4% of E and the NPV from 98.1% of S to 89.0% of in-house RdRp. All serological tests had < 50% sensitivity and low PPV and NPV. VivaDiag IgM (RDT) had 98.5% specificity, with 84.0% PPV, but 24.7% sensitivity. Conclusion: Molecular tests for SARS-CoV-2 infection showed excellent specificity, but significant differences in sensitivity. Serological tests have limited utility in a clinical context.
Generic Tasks for Algorithms
Authors: Milicic Gregor , Wetzel Sina , Ludwig Matthias
Published: September 2020

Due to its links to computer science (CS), teaching computational thinking (CT) often involves the handling of algorithms in activities, such as their implementation or analysis. Although there already exists a wide variety of different tasks for various learning environments in the area of computer science, there is less material available for CT. In this article, we propose so-called Generic Tasks for algorithms inspired by common programming tasks from CS education. Generic Tasks can be seen as a family of tasks with a common underlying structure, format, and aim, and can serve as best-practice examples. They thus bring many advantages, such as facilitating the process of creating new content and supporting asynchronous teaching formats. The Generic Tasks that we propose were evaluated by 14 experts in the field of Science, Technology, Engineering, and Mathematics (STEM) education. Apart from a general estimation in regard to the meaningfulness of the proposed tasks, the experts also rated which and how strongly six core CT skills are addressed by the tasks. We conclude that, even though the experts consider the tasks to be meaningful, not all CT-related skills can be specifically addressed. It is thus important to define additional tasks for CT that are detached from algorithms and programming.
Numerical Study on the Flow and Heat Transfer Coupled in a Rectangular Mini-Channel by Finite Element Method for Industrial Micro-Cooling Technologies
Authors: Kamdem Kamdem Claude Aurélien , Zhu Xiaolu
Published: September 2020

Nowadays, cooling high thermal flows in compact volumes continues to be one of the crucial problems in the industry. With the advent of advanced technologies, much more attention has been paid to how to improve the performance of cooling systems in the area of micro-technologies. Rectangular mini-channels are typical representatives which commonly used for cooling applications. However, micro-technologies still face the problem of low performance due to the low productivity of cooling related to unbefitting physical parameter values. Here, this work studies the applicability of the heat transfer scheme of convective flow and flow boiling in a rectangular mini-channel for satisfying the cooling requirement of industrial micro-technologies, through a simulation model governed by the coupled mechanism from Navier-Stokes (N-S) equation and heat transfer equations with phase change effect. In this work, various hydraulic diameters and different inlet fluid speed are used to calculate the different velocity profiles, pressure drops, coefficients of friction and finally, the distribution of the temperatures and dissipated heat flux. The simulation results show the applicability of the rectangular mini-channel in diverse applications such as engine cooling, electronic components, automotive on-board electronics and aerospace engineering. Flow boiling simulation results reveal that the obtained patterns were smooth mixture flow and discrete flow. The dissipated heat flux can reach 1.02–5.34 MW/m2 for a hydraulic diameter of 0.5 mm. We show that the system with the gradient temperature that evolves increasingly along the top and bottom walls of the channels presents the highest heat flux dissipated in flow boiling. Additionally, the fin efficiency of the system is 0.88 and the coefficient value of convective heat transfer is in the range between 5000 < h < 100,000, which indicates the flow boiling heat transfer is effective in the mini-channel when the Reynolds number is less than 400. It provides a significant heat exchange for cooling in these application areas.
Returning to Our Roots: Tribal Health and Wellness through Land-Based Healing
Authors: Johnson-Jennings Michelle , Billiot Shanondora , Walters Karina
Published: September 2020

(1) Background: Settler colonialism has severely disrupted Indigenous ancestral ways of healing and being, contributing to an onslaught of health disparities. In particular, the United Houma Nation (UHN) has faced large land loss and trauma, dispossession, and marginalization. Given the paucity of research addressing health for Indigenous individuals living in Louisiana, this study sought to co-identify a United Houma Nation health framework, by co-developing a community land-based healing approach in order to inform future community-based health prevention programs. (2) Methods: This pilot tested, co-designed and implemented a land-based healing pilot study among Houma women utilizing a health promotion leadership approach and utilized semi-structured interviews among 20 UHN women to identify a UHN health framework to guide future results. (3) Results: The findings indicated that RTOR was a feasible pilot project. The initial themes were (1.) place, (2.) environmental/land trauma, (3.) ancestors, (4.) spirituality/mindfulness, (5.) cultural continuity, and (6.) environment and health. The reconnection to land was deemed feasible and seen as central to renewing relationships with ancestors (aihalia asanochi taha), others, and body. This mindful, re-engagement with the land contributed to subthemes of developing stronger tribal identities, recreating ceremonies, and increased cultural continuity, and transforming narratives of trauma into hope and resilience. Based on these findings a Houma Health (Uma Hochokma) Framework was developed and presented. (4) Conclusions: Overall, this study found that land can serve as a feasible therapeutic site for healing through reconnecting Houma tribal citizens to both ancestral knowledges and stories of resilience, as well as viewing self as part of a larger collective. These findings also imply that revisiting historically traumatic places encouraged renewed commitment to cultural continuity and health behaviors—particularly when these places are approached relationally, with ceremony, and traumatic events tied to these places, including climate change and environmental/land trauma, are acknowledged along with the love the ancestors held for future generations.
The Target of Rapamycin Signalling Pathway in Ageing and Lifespan Regulation
Authors: Bjedov Ivana , Rallis Charalampos
Published: September 2020

Ageing is a complex trait controlled by genes and the environment. The highly conserved mechanistic target of rapamycin signalling pathway (mTOR) is a major regulator of lifespan in all eukaryotes and is thought to be mediating some of the effects of dietary restriction. mTOR is a rheostat of energy sensing diverse inputs such as amino acids, oxygen, hormones, and stress and regulates lifespan by tuning cellular functions such as gene expression, ribosome biogenesis, proteostasis, and mitochondrial metabolism. Deregulation of the mTOR signalling pathway is implicated in multiple age-related diseases such as cancer, neurodegeneration, and auto-immunity. In this review, we briefly summarise some of the workings of mTOR in lifespan and ageing through the processes of transcription, translation, autophagy, and metabolism. A good understanding of the pathway’s outputs and connectivity is paramount towards our ability for genetic and pharmacological interventions for healthy ageing and amelioration of age-related disease.
The Mediterranean Tourist City Reconsidered
Authors: Lekakis Stelios , Chatzikonstantinou Katerina
Published: September 2020

A man crosses the street holding his donkey by its bridle [...]
Prejudice and Feeling of Threat towards Syrian Refugees: The Moderating Effects of Precarious Employment and Perceived Low Outgroup Morality
Authors: Vallejo-Martín Macarena , Canto Jesús M. , San Martín García Jesús E. , Perles Novas Fabiola
Published: September 2020

Refugees frequently experience traumatic situations that result in the deterioration of their psychological well-being. In addition, perceived prejudice and discrimination against them by the host society can worsen their mental health. In this research study, using a Spanish sample, prejudice towards Syrian refugees is analyzed taking into account feeling of threat (realistic or symbolic), precarious employment, and perceived outgroup morality. Using a total of 365 participants, our results reveal that individuals feel more prejudice towards refugees when the former scored higher in realistic threat and symbolic threat, were in a highly precarious situation of employment and perceived refugees as being more immoral. Furthermore, it was found that persons who scored high in realistic threat and at the same time were in a situation of precarious employment, were those who displayed greater prejudice. The results likewise pointed to individuals who scored high in symbolic threat and in outgroup morality as being those who felt greater rejection towards the refugees. Accordingly, our results confirm the importance of feeling of threat in relation to prejudice, and highlight two important moderating factors: precarious employment and perceived outgroup morality.
Naturalistic Driving Study in Brazil: An Analysis of Mobile Phone Use Behavior while Driving
Authors: Bastos Jorge Tiago , Santos Pedro Augusto B. dos , Amancio Eduardo Cesar , Gadda Tatiana Maria C. , Ramalho José Aurélio , King Mark J. , Oviedo-Trespalacios Oscar
Published: September 2020

Mobile phone use (MPU) while driving is an important road safety challenge worldwide. Naturalistic driving studies (NDS) emerged as one of the most sophisticated methodologies to investigate driver behavior; however, NDS have not been implemented in low- or middle-income countries. The aim of this research is to investigate MPU while driving and compare the results to those reported in international studies. An analysis of 61.32 h and 1350 km driven in Curitiba (Brazil) showed that MPU lasted for an average of 28.51 s (n = 627) and occurred in 58.71% of trips (n = 201) with an average frequency of 8.37 interactions per hour (n = 201). The proportion of the trip time using a mobile phone was 7.03% (n = 201), and the average instantaneous speed was 12.77 km/h (n = 627) while using the phone. Generally, drivers spent less time on more complex interactions and selected a lower speed when using the phone. MPU was observed more during short duration than longer trips. Drivers in this study engaged in a larger number of MPU compared to drivers from Netherlands and the United States; and the percentage of trip time with MPU was between North American and European values.
Children’s Single-Leg Landing Movement Capability Analysis According to the Type of Sport Practiced
Authors: Estevan Isaac , Monfort-Torres Gonzalo , Farana Roman , Zahradnik David , Jandacka Daniel , García-Massó Xavier
Published: September 2020

(1) Background: Understanding children’s motor patterns in landing is important not only for sport performance but also to prevent lower limb injury. The purpose of this study was to analyze children’s lower limb joint angles and impact force during single-leg landings (SLL) in different types of jumping sports using statistical parametric mapping (SPM). (2) Methods: Thirty children (53.33% girls, M = 10.16 years-old, standard deviation (SD) = 1.52) divided into three groups (gymnastics, volleyball and control) participated in the study. The participants were asked to do SLLs with the dominant lower limb (barefoot) on a force plate from a height of 25 cm. The vertical ground reaction force (GRF) and lower limb joint angles were assessed. SPM{F} one-way analysis of variance (ANOVA) and SPM{t} unpaired t-tests were performed during the landing and stability phases. (3) Results: A significant main effect was found in the landing phase of jumping sport practice in GRF and joint angles. During the stability phase, this effect was exhibited in ankle and knee joint angles. (4) Conclusions: Evidence was obtained of the influence of practicing a specific sport in childhood. Child volleyball players performed SLL with lower impact force and higher knee flexion than child gymnasts. Training in specific jumping sports (i.e., volleyball and gymnastics) could affect the individual capacity to adapt SLL execution.
Enhancement of Toxic Efficacy of Alkylated Polycyclic Aromatic Hydrocarbons Transformed by Sphingobium quisquiliarum
Authors: Lee So-Young , Kwon Jung-Hwan
Published: September 2020

Alkylated polycyclic aromatic hydrocarbons (PAHs) are abundant in crude oils and refined petroleum products and are considered as major contributors to the toxicity of spilled oils. In this study, the microbial degradation of model (alkylated) PAHs (i.e., phenanthrene, 3-methylphenanthrene, 3,6-dimethylphenanthrene (36DMPhe), pyrene, and 1-methylpyrene (1MP)) by the bacterium Sphingobium quisquiliarum EPA505, a known degrader of PAHs, was studied. To evaluate the toxic potential of the metabolic products, reaction mixtures containing metabolites of 36DMPhe and 1MP were fractionated by high-performance liquid chromatography, and their effects on the luminescence inhibition of Aliivibrio fischeri were evaluated. Although the luminescence inhibition of 36DMPhe and 1MP at their solubility levels was not observed, inhibition was observed in their metabolite fractions at the solubility limit of their parent molecule. This indicates that initial biotransformation increases the toxicity of alkylated PAHs because of the increased solubility and/or inherent toxicity of metabolites. Qualitative analysis of the metabolite fractions suggested that mono-oxidation of the methyl group is the main metabolic pathway of 36DMPhe and 1MP.
Evaluation of a Suicide Prevention Program for the Energy Sector
Authors: Ross Victoria , Caton Neil , Mathieu Sharna , Gullestrup Jorgen , Kõlves Kairi
Published: September 2020

There is evidence indicating that traditionally male-dominated occupations are associated with greater risk of suicide. In Australia, MATES in Construction was developed as an occupational health initiative to prevent suicides in the industry. The program has recently been applied to the energy industry; however, little is known regarding exposure to suicide and suicide prevention interventions in this sector. The study aimed to examine the effectiveness of MATES in Energy general awareness training (GAT), and estimate the prevalence of recent suicidal ideation and exposure to suicidal behaviors in workers. A before and after design was used to examine the effectiveness of GAT training. Data were collected from 4887 participants undertaking GAT training at energy sites across Queensland, Australia. In total, 2% (97) of participants reported recent suicidal thoughts, 65% of participants reported they had known someone who had attempted suicide, and 69% had known someone who died by suicide. Significant improvements were found on all suicide literacy items after GAT training. Younger people were more likely to be positively affected by the intervention. The results indicate that the MATES in Energy program is successfully transitioning from the construction industry, and offers the first empirically supported suicide intervention tailored to the energy sector.
Effect of the Frustration of Psychological Needs on Addictive Behaviors in Mobile Videogamers—The Mediating Role of Use Expectancies and Time Spent Gaming
Authors: Chamarro Andrés , Oberst Ursula , Cladellas Ramón , Fuster Héctor
Published: September 2020

Casual videogames (CVGs), played on smartphones, are becoming increasingly popular, especially among females and adults. Whereas the addictive potential of online (computer) videogames is well-established, there is yet insufficient evidence for Internet gaming disorder (IGD) in mobile gamers and for the mediating role of some mechanisms involved. The aim of this study is to investigate the impact of the frustration of psychological needs on mobile videogamers’ addictive behavior as well as the role of cognitions (game-use expectancies) and behaviors (time spent playing) through a hypothesized serial mediation model, while controlling for important correlates, such as game genre, age, gender and payment during play. A total of 471 mobile game users (211 males) with an average age of 21.73 replied to an online survey containing sociodemographic and game variables, the Need Satisfaction and Frustration Scale (NSFS), the Internet Gaming Disorder Scale-Short Form (IGDS9-SF) and a slightly modified version of the Internet Use Expectancies Scale (IUES). The results corroborate the negative effects of need frustration on IGD among mobile gamers and clarify the role of time spent playing and game-use expectancies in the development of IGD, highlighting the important role of cognitions in this relationship. We conclude that both the time spent playing and game-use expectancies should be important targets for clinical interventions, even though they are not included in the diagnostic criteria.
Perception of Menstrual Normality and Abnormality in Spanish Female Nursing Students
Authors: Abreu-Sánchez Ana , Parra-Fernández María Laura , Onieva-Zafra María Dolores , Fernández-Martínez Elia
Published: September 2020

Menstrual problems are usually taboo; and often, some, such as dysmenorrhea, are presumed normal. This study seeks to compare the menstrual characteristics and symptoms of female university students reporting self-perceived normality concerning their cycles and menstruation with those who perceive their menstruation as being abnormal. A cross-sectional descriptive study was conducted among 270 nursing students using a self-report questionnaire that included sociodemographic and gynecological issues, together with Visual Analog Scale, the Andersch and Milsom Scale, and the Spanish version of the EuroQol-5 Dimension (EuroQol-5D) to measure self-perceived health status. A bivariate analysis was performed using the chi-square test, linear trend chi-square, and Student’s t-test, and a multivariate analysis of stepwise binary logistic regression was performed to predict the perception of cycle abnormality. In total, 77.4% of participants displayed normality; however, in self-reporting of menstrual characteristics, 67.4% identified alterations. Young women suffering from menstrual dizziness were 1.997 (CI95% = 1.010–3.950; p = 0.047) more likely to manifest abnormal menstruation, 4.518 (CI95% = 1.239–16.477; p = 0.022) more likely if they suffered from Grade 3 menstrual pain, and 2.851 (CI95% = 1.399–5.809; p = 0.004) more likely if they perceived that menstruation interfered with their daily lives. Many menstrual changes and symptoms are still considered normal, making it difficult to identify and address these issues. Therefore, it is necessary to develop health policies and strategies to improve menstrual health literacy for increased knowledge and earlier diagnosis.
Newborn Screening for Mucopolysaccharidosis Type II in Illinois: An Update
Authors: Burton Barbara K. , Hickey Rachel , Hitchins Lauren
Published: September 2020

Mucopolysaccharidosis type II (MPS II, Hunter syndrome) is a rare, progressive multisystemic lysosomal storage disorder with significant morbidity and premature mortality. Infants with MPS II develop signs and symptoms of the disorder in the early years of life, yet diagnostic delays are very common. Enzyme replacement therapy is an effective treatment option. It has been shown to prolong survival and improve or stabilize many somatic manifestations of the disorder. Our initial experience with newborn screening in 162,000 infants was previously reported. Here, we update that experience with the findings in 339,269 infants. Measurement of iduronate-2-sulfatase (I2S) activity was performed on dried blood spot samples submitted for other newborn screening disorders. A positive screen was defined as I2S activity less than or equal to 10% of the daily median. In this series, 28 infants had a positive screening test result, and four other infants had a borderline result. Three positive diagnoses of MPS II were established, and 25 were diagnosed as having I2S pseudodeficiency. The natural history and the clinical features of MPS II make it an ideal target for newborn screening. Newborn screening was effective in identifying affected infants in our population with an acceptable rate of false positive results.
Differential Expression of Circulating Plasma miRNA-370 and miRNA-10a from Patients with Hereditary Hemorrhagic Telangiectasia
Authors: Ruiz-Llorente Lidia , Albiñana Virginia , Botella Luisa M. , Bernabeu Carmelo
Published: September 2020

Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant, vascular disorder that presents with telangiectases and arteriovenous malformations. HHT is a genetically heterogeneous disorder, involving mutations in endoglin (ENG; HHT1) and activin receptor-like kinase 1 (ACVRL1/ALK1; HHT2) genes that account for over 85% of all HHT patients. The current diagnosis of HHT patients remains at the clinical level, but many suspected patients do not have a clear HHT diagnosis or do not show pathogenic mutations in HHT genes. This situation has prompted the search for biomarkers to help in the early diagnosis of the disease. We have analyzed the plasma levels in HHT patients of selected micro-RNAs (miRNAs), small single-stranded RNAs that regulate gene expression at the transcriptional level by interacting with specific RNA targets. A total of 16 HHT1 and 17 HHT2 plasma samples from clinically confirmed patients and 16 controls were analyzed in this study. Total RNA was purified from plasma, and three selected miRNAs (miRNA-10a, miRNA-214, and miRNA-370), related to the pathobiology of cardiovascular diseases and potentially targeting ENG or ALK1, were measured by quantitative polymerase chain reaction. Compared with controls, levels of miRNA-370, whose putative target is ENG, were significantly downregulated in HHT1, but not in HHT2, whereas the levels of miRNA-10a, whose putative target is ALK1, were significantly upregulated in HHT2, but not in HHT1. In addition, the levels of miRNA-214, potentially targeting ENG and ALK1, did not change in either HHT1 or HHT2 patients versus control samples. While further studies are warranted, these results suggest that dysregulated plasma levels of miRNA-370 or miRNA-10a could help to identify undiagnosed HHT1 or HHT2 patients, respectively.
Induction of Micronuclei in Cervical Cancer Treated with Radiotherapy
Authors: Kobayashi Daijiro , Oike Takahiro , Murata Kazutoshi , Irie Daisuke , Hirota Yuka , Sato Hiro , Shibata Atsushi , Ohno Tatsuya
Published: September 2020

Micronuclei (MN) trigger antitumor immune responses via the cyclic GMP-AMP synthase-signaling effector stimulator of interferon genes (cGAS-STING) pathway. Radiotherapy induces MN in peripheral blood lymphocytes. However, data for solid tumors are lacking. Here, we analyzed MN post-radiotherapy in solid tumor samples. Tumor biopsy specimens were obtained from seven prospectively recruited patients with cervical cancer, before treatment and after receiving radiotherapy at a dose of 10 Gy (in five fractions). The samples were stained with 4′,6-diamidino-2-phenylindole dihydrochloride, and 200 nuclei per sample were randomly identified and assessed for the presence of MN or apoptosis, based on nuclear morphology. The median number of MN-harboring nuclei was significantly greater in samples from patients treated with radiotherapy than in pre-treatment samples (151 (range, 16–327) versus 28 (range, 0–61); p = 0.015). No significant differences in the number of apoptotic nuclei were observed between pre-treatment and 10 Gy samples (5 (range, 0–30) versus 12 (range, 2–30); p = 0.30). This is the first report to demonstrate MN induction by radiotherapy in solid tumors. The results provide clinical evidence of the activation of antitumor immune responses by radiotherapy.
Using the SWOT Methodology to Know the Scope of the Digitalization of the Spanish Ports
Authors: González-Cancelas Nicoleta , Molina Serrano Beatriz , Soler-Flores Francisco , Camarero-Orive Alberto
Published: September 2020

In the specific case of maritime transport, one of the most notable examples of digitalization is smart ports. Their positive impact on the supply chain is enabling processes to be optimized through smarter production. In the case of Spain, the Spanish public entity Puertos del Estado (Puertos del Estado is a public business entity dependent on the Spanish Ministry of Transport, Mobility and the Urban Agenda, with overall responsibility for the entire state-owned port system.) has launched Ports 4.0, the largest open innovation fund for the logistics-port ecosystem. It is therefore necessary to know the point of digitalization in which the Spanish ports are and the present scenario in which they develop their activity. For this purpose, a SWOT analysis (Strengths-Weaknesses-Opportunities-Threats analysis) is proposed, which is an ideal tool to make a reliable diagnosis of the port system in relation to a certain project before which we wish to make a strategic decision, in this case digitalization, in order to establish a concrete action plan in this regard. This should be the first step towards developing the Ports 4.0 project. The main conclusion of the analysis is that Spanish ports are currently at a medium high level of digitalization, and that they continue to update and innovate in order to be increasingly competitive in this market, despite the fact that there is still a long way to go in digitalization. The main challenge for Spanish ports is that technology alone is not useful enough to reach Ports 4.0 status; the technology also needs to be used in an integrated, open and joint way by all the agents of the port community, which is one of the main handicaps at present.
Quantifying 3D Strain in Scaffold Implants for Regenerative Medicine
Authors: Clark Jeffrey N. , Tavana Saman , Heyraud Agathe , Tallia Francesca , Jones Julian R. , Hansen Ulrich , Jeffers Jonathan R. T.
Published: September 2020

Regenerative medicine solutions require thoughtful design to elicit the intended biological response. This includes the biomechanical stimulus to generate an appropriate strain in the scaffold and surrounding tissue to drive cell lineage to the desired tissue. To provide appropriate strain on a local level, new generations of scaffolds often involve anisotropic spatially graded mechanical properties that cannot be characterised with traditional materials testing equipment. Volumetric examination is possible with three-dimensional (3D) imaging, in situ loading and digital volume correlation (DVC). Micro-CT and DVC were utilised in this study on two sizes of 3D-printed inorganic/organic hybrid scaffolds (n = 2 and n = 4) with a repeating homogenous structure intended for cartilage regeneration. Deformation was observed with a spatial resolution of under 200 µm whilst maintaining displacement random errors of 0.97 µm, strain systematic errors of 0.17% and strain random errors of 0.031%. Digital image correlation (DIC) provided an analysis of the external surfaces whilst DVC enabled localised strain concentrations to be examined throughout the full 3D volume. Strain values derived using DVC correlated well against manually calculated ground-truth measurements (R2 = 0.98, n = 8). The technique ensures the full 3D micro-mechanical environment experienced by cells is intimately considered, enabling future studies to further examine scaffold designs for regenerative medicine.
Theoretical Analysis of Blast Protection of Graded Metal Foam-Cored Sandwich Cylinders/Rings
Authors: Liang Minzu , Li Xiangyu , Lin Yuliang , Zhang Kefan , Lu Fangyun
Published: September 2020

The blast resistance of a sandwich-walled cylinder/ring comprising two metal face-sheets and a graded metal foam core, subjected to internal air blast loading, is investigated. Analytical models are developed for the deformation of the sandwich cylinder with positive and negative gradient cores under internal blast loading. The deformation process is divided into three distinct phases, namely the fluid–structure interaction phase, core-crushing phase, and outer face-sheet deformation phase. Finite element modeling is performed using the Voronoi material model. The proposed analytical models are verified through finite element analysis, and reasonable agreement is observed between the analytical predictions and finite element results. The sandwich structures with high energy absorption capacity or low maximum radial deflection are satisfied for the protecting purpose of impact/blast resistance requirements. Typical deformation processes are classified and analyzed; the effects of explosive charge, face-sheet thickness, and core gradient on the structural response are also examined. The results indicate that both the deformation modes and the structural response of the cylinders are sensitive to the blast charge and core configuration. It is concluded that energy absorption capacity and maximum radial deflection are two conflicting goals for achieving high impact/blast resistance capability. An in-depth understanding of the behavior in sandwich-walled cylinders under blast impulse and the influence of the core configuration helps realize the advantages and disadvantages of using graded foam materials in sandwich structures and can provide a guideline for structural design.
Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration
Authors: Prima Giulia , Librizzi Fabio , Carrotta Rita
Published: September 2020

Over the last few decades, liposomes have emerged as promising drug delivery systems and effective membrane models for studying biophysical and biological processes. For all applications, knowing their concentration after preparation is crucial. Thus, the development of methods for easily controlling vesicles concentration would be of great utility. A new assay is presented here, based on a suitable analysis of light scattering intensity from liposome dispersions. The method, tested for extrusion preparations, is precise, easy, fast, non-destructive and uses a tiny amount of sample. Furthermore, the scattering intensity can be measured indifferently at different angles, or even by using the elastic band obtained from a standard spectrofluorimeter. To validate the method, the measured concentrations of vesicles of different matrix compositions and sizes, measured by light scattering with different angles and instruments, were compared to the data obtained by the standard Stewart assay. Consistent results were obtained. The light scattering assay is based on the assessment of the mass fraction lost in the preparation, and can be applied for methods such as extrusion, homogenization, French press and other microfluidic procedures.