Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregativeEscherichia coliO104:H4 outbreak strain
Authors: Whelan Rachel , Cyganek Martyna , Dickins Benjamin , Thomas Jonathan C , McVicker Gareth 
Published: November 2024

EnteroaggregativeEscherichia coliO104:H4 is best known for causing a worldwide outbreak in 2011 due to acquisition of a Shiga-like toxin alongside traditional enteroaggregative virulence traits; however, whilst the 2011 outbreak strain has been well-studied, the virulence plasmid of O104:H4 has been subjected to far less experimental analysis. In this paper, we analyse the genetic and phenotypic contribution of the pAA virulence plasmid to a non-Shigatoxigenic O104:H4 strain (1070/13) that was nonetheless implicated in a substantial UK outbreak in 2013. We find that pAA1070is 99.95% identical across 88% of the plasmid sequence to pTY2 from the 2011 outbreak strain and has a copy number of approximately 2-3 plasmid molecules per chromosome. We demonstrate that pAA1070carries a functional CcdAB plasmid addiction system that only marginally impacts its stability under the conditions tested and that none of the other toxin-antitoxin systems encoded by the plasmid appear to be functional, though we note a surprisingly high stability of the plasmidin vitroregardless. We demonstrate the expected contribution of pAA1070to intestinal cell adhesion but find that it does not contribute to biofilm formation. When assessing the impact of pAA1070on motility, we discovered a region of the O104:H4 chromosome that can be excised, abolishing motility via truncation of thefliRgene. Ultimately this work contributes to our knowledge of enteroaggregativeE. colias an important pathovar in its own right and demonstrates the complexity but necessity of experimentally characterising genuine outbreak strains rather than domesticated laboratory strains in order to understand virulence phenotypes.

IMPACT STATEMENT

EnteroaggregativeEscherichia colicauses severe food-borne illness in humans. One serotype, O104:H4, caused a worldwide outbreak in 2011 that infected almost 4,000 people and killed dozens. The 2011 strain contained two key virulence factors: the Shiga toxin encoded by a bacteriophage and a plasmid that encodes aggregative adherence fimbriae for adhesion to host cell surfaces. Whilst the Shiga toxin has been studied extensively, the virulence plasmid has been far less well-characterised. In this study, we characterise the plasmid of a non-Shigatoxigenic O104:H4 outbreak strain and find that it contributes to host cell adherence, as expected, and that it encodes a functional CcdAB toxin-antitoxin stability system. We also demonstrate that part of the strain’s chromosome can be spontaneously lost during growth in the laboratory, converting the organism from highly-motile to non-motile, and suggesting a future evolutionary route for the bacterium, as has occurred in similar enteric pathogens such asShigella. In summary, this work contributes to our knowledge of often-neglected enteroaggregativeE. colias an important pathovar and highlights the necessity of experimentally characterising genuine outbreak strains rather than “domesticated” laboratory strains.

Repositories/Data Summary

The authors confirm all supporting data, code and protocols have been provided within the article or through supplementary data files. Hybrid whole-genome sequence data is available for strains 1070/13 (accession numbersCP171474,CP171475andCP171476) and 1070/13 pAA−(accession numbersCP171472andCP171473). Sequencing data for 1070/13 produced by Dallmanet al.(2014) is available from the European Nucleotide Archive: sample accession SAMN02730196.