bluuebunny/crossref_metadata_2025 huggingface dataset:
Symphonies of Growth: Unveiling the Impact of Sound Waves on Plant Physiology and Productivity
Authors: Pagano Mario , Del Prete Sonia
Published: May 2024

The application of sound wave technology to different plant species has revealed that variations in the Hz, sound pressure intensity, treatment duration, and type of setup of the sound source significantly impact the plant performance. A study conducted on cotton plants treated with Plant Acoustic Frequency Technology (PAFT) highlighted improvements across various growth metrics. In particular, the treated samples showed increases in the height, size of the fourth expanded leaf from the final one, count of branches carrying bolls, quantity of bolls, and weight of individual bolls. Another study showed how the impact of a 4 kHz sound stimulus positively promoted plant drought tolerance. In other cases, such as in transgenic rice plants, GUS expression was upregulated at 250 Hz but downregulated at 50 Hz. In the same way, sound frequencies have been found to enhance the osmotic potential, with the highest observed in samples treated with frequencies of 0.5 and 0.8 kHz compared to the control. Furthermore, a sound treatment with a frequency of 0.4 kHz and a sound pressure level (SPL) of 106 dB significantly increased the paddy rice germination index, as evidenced by an increase in the stem height and relative fresh weight. This paper presents a complete, rationalized and updated review of the literature on the effects of sound waves on the physiology and growth parameters of sound-treated plants.
The Urine Light Chain/eGFR Quotient as a Tool to Rule out Cast Nephropathy in Myeloma-Associated Kidney Failure
Authors: Klank David , Löffler Christian , Friedrich Julian , Hoffmann Martin , Paschka Peter , Bergner Raoul
Published: May 2024

Kidney involvement with resulting kidney failure leads to increased mortality in patients with multiple myeloma (MM). Cast nephropathy (CN), in particular, if left untreated, quickly leads to kidney failure requiring dialysis and has a very poor prognosis for the affected patient. The gold standard for diagnosing kidney involvement is a kidney biopsy. However, due to bleeding risk, this cannot be done in every patient. We recently reported that a quotient of urine light chain (LCurine) to glomerular filtration rate (eGFR) is a non-invasive diagnostic tool for patients with kidney involvement in MM. But this quotient has not yet been tested in everyday clinical practice. In this study, our LCurine/eGFR ratio was tested on 67 patients in two centers. Enrollment took place between January 2019 and September 2023. A total of 18 of the 67 patients had CN. With the threshold defined in our initial paper, we were able to show a sensitivity of 100% with a specificity of 85.7% for CN in patients with MM. As a result, the LCurine/eGFR quotient recognizes 100% of all CN and can therefore detect this group, which has a very poor prognosis, without the need for a kidney biopsy.
A Microactuator Array Based on Ionic Electroactive Artificial Muscles for Cell Mechanical Stimulation
Authors: Gu Jing , Zhou Zixing , Xie Yang , Zhu Xiaobin , Huang Guoyou , Zhang Zuoqi
Published: May 2024

Mechanical stimulation is prevalent within organisms, and appropriate regulation of such stimulation can significantly enhance cellular functions. Consequently, the in vitro construction and simulation of mechanical stimulation have emerged as a research hotspot in biomechanics. In recent years, a class of artificial muscles named electroactive polymers (EAPs), especially ionic EAPs, have shown promising applications in biomechanics. While several techniques utilizing ionic EAPs for cell mechanical stimulation have been reported, further research is needed to advance and enhance their practical applications. Here, we prepared a microactuator array based on ionic EAP artificial muscles for cell mechanical stimulation. As a preliminary effort, we created a 5 × 5 microactuator array on a supporting membrane by employing laser cutting. We evaluated the electro-actuation performance of the microactuators through experimental testing and numerical simulations, affirming the potential use of the microactuator array for cell mechanical stimulation. The devised approach could inspire innovative design concepts in the development of miniaturized intelligent electronic devices, not only in biomechanics and biomimetics but also in other related fields.
Catalytic Hairpin Assembly-Based Self-Ratiometric Gel Electrophoresis Detection Platform for Reliable Nucleic Acid Analysis
Authors: Xi Qiang , Wang Si-Yi , Deng Xiao-Bing , Zhang Chong-Hua
Published: May 2024

The development of gel electrophoresis-based biodetection assays for point-of-care analysis are highly demanding. In this work, we proposed a ratiometric gel electrophoresis-based biosensing platform by employing catalytic hairpin assembly (CHA) process functions as both the signal output and the signal amplification module. Two types of nucleic acids, DNA and miRNA, are chosen for demonstration. The proposed strategy indeed provides a new paradigm for the design of a portable detection platform and may hold great potential for sensitive diagnoses.
The Development of a Specific Nanofiber Bioreceptor for Detection of Escherichia coli and Staphylococcus aureus from Air
Authors: Varvařovská Leontýna , Kudrna Petr , Sopko Bruno , Jarošíková Taťána
Published: May 2024

Polluted air and the presence of numerous airborne pathogens affect our daily lives. The sensitive and fast detection of pollutants and pathogens is crucial for environmental monitoring and effective medical diagnostics. Compared to conventional detection methods (PCR, ELISA, metabolic tests, etc.), biosensors bring a very attractive possibility to detect chemicals and organic particles with the mentioned reliability and sensitivity in real time. Moreover, by integrating nanomaterials into the biosensor structure, it is possible to increase the sensitivity and specificity of the device significantly. However, air quality monitoring could be more problematic even with such devices. The greatest challenge with conservative and sensing methods for detecting organic matter such as bacteria is the need to use liquid samples, which slows down the detection procedure and makes it more difficult. In this work, we present the development of a polyacrylonitrile nanofiber bioreceptor functionalized with antibodies against bacterial antigens for the specific interception of bacterial cells directly from the air. We tested the presented novel nanofiber bioreceptor using a unique air filtration system we had previously created. The prepared antibody-functionalized nanofiber membranes for air filtration and pathogen detection (with model organisms E. coli and S. aureus) show a statistically significant increase in bacterial interception compared to unmodified nanofibers. Creating such a bioreceptor could lead to the development of an inexpensive, fast, sensitive, and incredibly selective bionanosensor for detecting bacterial polluted air in commercial premises or medical facilities.
A Machine Learning-Based Approach for Predicting Installation Torque of Helical Piles from SPT Data
Authors: Peres Marcelo Saraiva , Schiavon José Antonio , Ribeiro Dimas Betioli
Published: May 2024

Helical piles are advantageous alternatives in constructions subjected to high tractions in their foundations, like transmission towers. Installation torque is a key parameter to define installation equipment and the final depth of the helical pile. This work applies machine learning (ML) techniques to predict helical pile installation torque based on information from 707 installation reports, including Standard Penetration Test (SPT) data. It uses this information to build three datasets to train and test eight machine-learning techniques. Decision tree (DT) was the worst technique for comparing performances, and cubist (CUB) was the best. Pile length was the most important variable, while soil type had little relevance for predictions. Predictions become more accurate for torque values greater than 8 kNm. Results show that CUB predictions are within 0.71,1.59 times the real value with a 95% confidence. Thus, CUB successfully predicted the pile length using SPT data in a case study. One can conclude that the proposed methodology has the potential to aid in the helical pile design and the equipment specification for installation.
Complexity, Coordination Dynamics and the Urban Landscape
Authors: Portugali Juval
Published: May 2024

Similar to living complex systems, cities are composed of a huge number of interacting parts, each with its specific properties, rhythm, etc., that, by means of self-organization, give rise to a functioning complex system. A major challenge is thus to follow the self-organized adaptation process by which the huge number of diverse parts coordinate their action and behavior into a coherent whole. Coordination dynamics, the science of coordination, elaborates on this issue, showing how patterns of coordination form, adapt, persist and change in living things. Recent studies on cities and complexity exposed that human agents differ from other living things in that they adapt not only through behavior but also through the construction of artifacts, thus giving rise to hybrid complex systems (HCSs) and to cities as such. This entails a new challenge regarding the various aspects and roles of artifacts in coordination dynamics. This study introduces the notions of hybrid complex systems and coordination dynamics and then focuses on one aspect that concerns coordination in cities: the ways the artificial urban landscape participates in coordinating the dynamics between the human urban agents.
Study on the Influence of the Performance Weakening of the Disconnectable Coupling (DC) Joint of Steel Support on the Retaining Structure of a Foundation Pit
Authors: Xie Zhitian , Liu Xiao , Niu Xiaokai , Jian Jialong , Xu Chentao , Liao Jiahui
Published: May 2024

In order to quantitatively study the influence of the weakening of the disconnectable coupling joint (DC joint) on the retaining structure, the pre-axial force retention performance of the steel support, the axial force of the steel support, the horizontal displacement of the diaphragm wall, and the ground settlement around the foundation pit were monitored during the construction of the foundation pit. The evolution process of the monitoring data was analyzed, and the corresponding numerical model verified by the monitoring data was established. The influence of the yield load of the DC joint, the initial compression stiffness, and the weakening of the pre-axial force on the stability of the retaining structure was studied by numerical simulation. The results show that the pre-axial force of steel support is only 67% of the design value when the soil below is not excavated within 24 h. The DC joint has a significant weakening effect on the steel support, which is unfavorable for the stability control of the foundation pit retaining structure. The pre-axial force and initial bending stiffness have a great influence on the stability of the retaining structure. When the yield load is not lower than that of the row piles, the DC joint has no effect on the stability of the retaining structure. This model can predict and analyze the deformation trend under different working conditions to a certain extent, providing certain reference value for safety plans during construction.
Bearing Characteristics with Effect of Bond–Slip Behavior in Massive Ring-Type Reinforced Concrete Structures
Authors: Xu Wen-Tao , Ma Zhu , Wu He-Gao , Shi Chang-Zheng
Published: May 2024

The bond–slip behavior of the steel–concrete interface is critical in reinforced concrete (RC) structures since the bond action is the mechanism that ensures the two materials work in co-operation. However, there is little research considering the bond–slip behavior in massive ring-type reinforced concrete (MRRC) structure bearing analyses due to the complexity of modeling the interfacial behavior. Hence, the influence of the bond–slip behavior on the bearing characteristics of MRRC structures remains unclear. Steel-lined reinforced concrete penstock is such an MRRC structure, composed of steel liner and reinforced concrete and commonly used in diversion pipelines. This paper aims to explore the bearing characteristics considering the bond–slip behavior in the composite penstock by using a promising numerical method, the cohesive zone model. Three interface models were proposed to represent the different interaction conditions at the steel–concrete interface. Moreover, a sensitivity analysis was performed to study the impact of the bond strength on the bond performance and structural behavior. The simulation results showed that the prediction results (steel stress and crack process) considering the bond–slip behavior were in good agreement with the experimental results. The steel stresses near the cracks were smaller and more uniform after considering the bond–slip behavior, since the stresses were no longer concentrated on the crack but distributed in an area near the crack. However, the steel stress differences in these models were within 10%, which means that the bond performance had a limited effect on the structural safety design. The crack widths were greatly influenced by the bond conditions, and the maximum crack width (0.461 mm) in poor conditions was beyond the limiting value (0.3 mm). Consequently, bond–slip behavior must be paid more attention in durability design.
Radiation Safety Assessment in Prostate Cancer Treatment: A Predictive Approach for I-125 Brachytherapy
Authors: Chuang Ho-Da , Lin Yu-Hung , Lin Chin-Hsiung , Lai Yuan-Chun , Wu Chin-Hui , Hsu Shih-Ming
Published: May 2024

This study uses Monte Carlo simulation and experimental measurements to develop a predictive model for estimating the external dose rate associated with permanent radioactive source implantation in prostate cancer patients. The objective is to estimate the accuracy of the patient’s external dose rate measurement. First, I-125 radioactive sources were implanted into Mylar window water phantoms to simulate the permanent implantation of these sources in patients. Water phantom experimental measurement was combined with Monte Carlo simulation to develop predictive equations, whose performance was verified against external clinical data. The model’s accuracy in predicting the external dose rate in patients with permanently implanted I-125 radioactive sources was high (R2 = 0.999). A comparative analysis of the experimental measurements and the Monte Carlo simulations revealed that the maximum discrepancy between the measured and calculated values for the water phantom was less than 5.00%. The model is practical for radiation safety assessments, enabling the evaluation of radiation exposure risks to individuals around patients with permanently implanted I-125 radioactive sources.
Novel Imaging Approaches for Glioma Classification in the Era of the World Health Organization 2021 Update: A Scoping Review
Authors: Richter Vivien , Ernemann Ulrike , Bender Benjamin
Published: May 2024

The 2021 WHO classification of CNS tumors is a challenge for neuroradiologists due to the central role of the molecular profile of tumors. The potential of novel data analysis tools in neuroimaging must be harnessed to maintain its role in predicting tumor subgroups. We performed a scoping review to determine current evidence and research gaps. A comprehensive literature search was conducted regarding glioma subgroups according to the 2021 WHO classification and the use of MRI, radiomics, machine learning, and deep learning algorithms. Sixty-two original articles were included and analyzed by extracting data on the study design and results. Only 8% of the studies included pediatric patients. Low-grade gliomas and diffuse midline gliomas were represented in one-third of the research papers. Public datasets were utilized in 22% of the studies. Conventional imaging sequences prevailed; data on functional MRI (DWI, PWI, CEST, etc.) are underrepresented. Multiparametric MRI yielded the best prediction results. IDH mutation and 1p/19q codeletion status prediction remain in focus with limited data on other molecular subgroups. Reported AUC values range from 0.6 to 0.98. Studies designed to assess generalizability are scarce. Performance is worse for smaller subgroups (e.g., 1p/19q codeleted or IDH1/2 mutated gliomas). More high-quality study designs with diversity in the analyzed population and techniques are needed.
Angiogenesis Still Plays a Crucial Role in Human Melanoma Progression
Authors: Cazzato Gerardo , Ingravallo Giuseppe , Ribatti Domenico
Published: May 2024

Angiogenesis plays a pivotal role in tumor progression, particularly in melanoma, the deadliest form of skin cancer. This review synthesizes current knowledge on the intricate interplay between angiogenesis and tumor microenvironment (TME) in melanoma progression. Pro-angiogenic factors, including VEGF, PlGF, FGF-2, IL-8, Ang, TGF-β, PDGF, integrins, MMPs, and PAF, modulate angiogenesis and contribute to melanoma metastasis. Additionally, cells within the TME, such as cancer-associated fibroblasts, mast cells, and melanoma-associated macrophages, influence tumor angiogenesis and progression. Anti-angiogenic therapies, while showing promise, face challenges such as drug resistance and tumor-induced activation of alternative angiogenic pathways. Rational combinations of anti-angiogenic agents and immunotherapies are being explored to overcome resistance. Biomarker identification for treatment response remains crucial for personalized therapies. This review highlights the complexity of angiogenesis in melanoma and underscores the need for innovative therapeutic approaches tailored to the dynamic TME.
Effect of Tumor Regression Grade on Survival and Disease-Free Interval in Patients Operated on for Locally Advanced Rectal Cancer
Authors: Mendoza-Moreno Fernando , Díez-Alonso Manuel , Matías-García Belén , Ovejero-Merino Enrique , Vera-Mansilla Cristina , Quiroga-Valcárcel Ana , Blázquez-Martín Alma , Jiménez-Martín Rubén , Lasa-Unzúe Inmaculada , Ortega Miguel A. , Alvarez-Mon Melchor , Gutiérrez-Calvo Alberto
Published: May 2024

Introduction: Colorectal cancer is the fourth leading cause of cancer-related death in both men and women in our population. In this regard, rectal cancer accounts for more than half of colorectal cancer deaths, and its incidence is expected to increase in the coming years. There have been significant changes in neoadjuvant therapy regimens, with promising results, as demonstrated by the recent RAPIDO and PRODIGE23 studies. Around 40% of patients diagnosed with locally advanced rectal cancer show some degree of response to neoadjuvant treatment, with complete tumor regression observed in up to one in five patients. Materials and Methods: Retrospective observational study. A total of 181 patients with locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy followed by surgery were analyzed. Clinical and pathological data were collected from the patients, including assessment of tumor regression through histopathological studies after surgery. The Mandard tumor regression grading system was used to categorize tumor response into different grades. Results: The results showed a significant association between the degree of tumor regression and several important clinical outcomes. Specifically, patients with higher tumor regression had significantly better disease-free survival than those with less regression (p = 0.004). In addition, tumor regression was also correlated with the incidence of local recurrence (p = 0.018) and distant metastasis (p = 0.032). These associations suggest that tumor responsiveness to neoadjuvant therapy may influence the long-term progression of the disease. Regarding tumor deposits and the presence of lymphadenopathy, these factors were also found to be significantly associated with clinical outcomes. Patients with tumor deposits had a higher incidence of local recurrence (p = 0.025) and distant metastases (p = 0.041), while the presence of lymphadenopathy increased the risk of local recurrence (p = 0.013). These findings highlight the importance of evaluating not only tumor regression but also other pathological markers to predict prognosis and guide clinical management. Conclusions: The degree of tumor regression was not an independent predictor of survival compared to other variables such as nodal stage and presence of tumor deposits. This indicates that while tumor regression is an important factor, other elements also play a crucial role in determining the prognosis of patients with locally advanced rectal cancer. This study provides additional evidence for the importance of tumor regression, tumor deposits, and lymphadenopathy as predictors of clinical outcomes in patients with rectal cancer treated with neoadjuvant chemoradiotherapy.
Reconstruction of Partial Hypopharyngeal Defects following Total Laryngectomy: A Systematic Review and Meta-Analysis
Authors: Tonsbeek Anthony M. , Leidelmeijer Roxy , Hundepool Caroline A. , Duraku Liron S. , Van der Oest Mark J. W. , Sewnaik Aniel , Mureau Marc A. M.
Published: May 2024

Background: Various operative techniques exist to reconstruct partial hypopharyngeal defects following total laryngectomy. The current study aimed to investigate and compare complications and functional results following commonly used reconstructive techniques. Methods: A systematic review and meta-analysis were performed using studies that investigated outcomes after the reconstruction of a partial hypopharyngeal defect. The outcomes of interest were fistulas, strictures, flap failure, swallowing function and postoperative speech. Results: Of the 4035 studies identified, 23 were included in this review. Four common reconstructive techniques were reported, with a total of 794 patients: (1) pectoralis major myocutaneous and (2) myofascial flap, (3) anterolateral thigh free flap and (4) radial forearm free flap. Fistulas occurred significantly more often than pectoralis major myocutaneous flaps (34%, 95% CI 23–47%) compared with other flaps (p < 0.001). No significant differences in the rates of strictures or flap failure were observed. Pectoralis major myofascial flaps were non-inferior to free-flap reconstructions. Insufficient data were available to assess speech results between flap types. Conclusion: Pectoralis myocutaneous flaps should not be the preferred method of reconstruction for most patients, considering their significantly higher rate of fistulas. In contrast, pectoralis major myofascial flaps yield promising results compared to free-flap reconstructions, warranting further investigation.
Early Stage In Vitro Bioprofiling of Potential Low-Molecular-Weight Organoboron Compounds for Boron Neutron Capture Therapy (BNCT)—Proposal for a Guide
Authors: Leśnikowski Zbigniew J. , Ekholm Filip , Hosmane Narayan S. , Kellert Martin , Matsuura Eiji , Nakamura Hiroyuki , Olejniczak Agnieszka B. , Panza Luigi , Rendina Louis M. , Sauerwein Wolfgang A. G.
Published: May 2024

Given the renewed interest in boron neutron capture therapy (BNCT) and the intensified search for improved boron carriers, as well as the difficulties of coherently comparing the carriers described so far, it seems necessary to define a basic set of assays and standardized methods to be used in the early stages of boron carrier development in vitro. The selection of assays and corresponding methods is based on the practical experience of the authors and is certainly not exhaustive, but open to discussion. The proposed tests/characteristics: Solubility, lipophilicity, stability, cytotoxicity, and cellular uptake apply to both low molecular weight (up to 500 Da) and high molecular weight (5000 Da and more) boron carriers. However, the specific methods have been selected primarily for low molecular weight boron carriers; in the case of high molecular weight compounds, some of the methods may need to be adapted.
CRISPR-Based Gene Therapies: From Preclinical to Clinical Treatments
Authors: Laurent Marine , Geoffroy Marine , Pavani Giulia , Guiraud Simon
Published: May 2024

In recent years, clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) protein have emerged as a revolutionary gene editing tool to treat inherited disorders affecting different organ systems, such as blood and muscles. Both hematological and neuromuscular genetic disorders benefit from genome editing approaches but face different challenges in their clinical translation. The ability of CRISPR/Cas9 technologies to modify hematopoietic stem cells ex vivo has greatly accelerated the development of genetic therapies for blood disorders. In the last decade, many clinical trials were initiated and are now delivering encouraging results. The recent FDA approval of Casgevy, the first CRISPR/Cas9-based drug for severe sickle cell disease and transfusion-dependent β-thalassemia, represents a significant milestone in the field and highlights the great potential of this technology. Similar preclinical efforts are currently expanding CRISPR therapies to other hematologic disorders such as primary immunodeficiencies. In the neuromuscular field, the versatility of CRISPR/Cas9 has been instrumental for the generation of new cellular and animal models of Duchenne muscular dystrophy (DMD), offering innovative platforms to speed up preclinical development of therapeutic solutions. Several corrective interventions have been proposed to genetically restore dystrophin production using the CRISPR toolbox and have demonstrated promising results in different DMD animal models. Although these advances represent a significant step forward to the clinical translation of CRISPR/Cas9 therapies to DMD, there are still many hurdles to overcome, such as in vivo delivery methods associated with high viral vector doses, together with safety and immunological concerns. Collectively, the results obtained in the hematological and neuromuscular fields emphasize the transformative impact of CRISPR/Cas9 for patients affected by these debilitating conditions. As each field suffers from different and specific challenges, the clinical translation of CRISPR therapies may progress differentially depending on the genetic disorder. Ongoing investigations and clinical trials will address risks and limitations of these therapies, including long-term efficacy, potential genotoxicity, and adverse immune reactions. This review provides insights into the diverse applications of CRISPR-based technologies in both preclinical and clinical settings for monogenic blood disorders and muscular dystrophy and compare advances in both fields while highlighting current trends, difficulties, and challenges to overcome.
The Case for Multidisciplinary Frameworks for Developing Effective Solutions to Complex Human Problems: An Illustration Based on Development Education, Corporate Social Responsibility and Social Marketing
Authors: Fourali Chahid E.
Published: May 2024

Education, especially development education (DE), and a number of socially focused disciplines, including corporate social responsibility (CSR) and social marketing (SM), have long been targeted by policy makers for deriving advice on the ‘wisdom’ of levelling up differences and addressing sources of disadvantages at individual, group and/or regional levels. Additionally, the combined wisdom of such disciplines can also be a great source of advice to effectively address perennial universal problems. This paper is conceptual in nature with a multidisciplinary outlook. It contrasts DE, CSR and SM, with the view to deriving common grounds as well as strengths and areas for further development that can produce more comprehensive explanations and solutions to social problems. Such inclusive, more comprehensive explanations would help advise social-cause-focused workers, including researchers, learners and policy makers, about how each discipline can contribute to the resolution of multifaceted problems, the so-called ‘wicked problems’, that each discipline may not be fully equipped to address. The method of analysis used is an adjusted version of critical discourse analysis. It is used to explore the disciplines at four levels, namely definitional, philosophical, methodological and performance levels, thus giving a comprehensive view of each discipline’s nature, philosophical outlook, methodology and perceived efficacy in achieving its aims. The derived arguments also benefitted from comments provided by seven experienced representatives from the three disciplines. Overall, the outcomes suggest a relative maturity of critical ability in DE but also more effective and efficient methodological and evaluative perspectives in CSR and SM. Although the outcome of the analysis is open for debate, it nevertheless suggests several opportunities for mutual learning at all four levels. The paper suggests a novel integrated ‘supra-level’ framework that may help workers, in these three areas of knowledge, gain valuable insights from each of the three disciplines and highlight valuable opportunities for capitalising on their respective strengths.
A Cold Flow Model of Interconnected Slurry Bubble Columns for Sorption-Enhanced Fischer–Tropsch Synthesis
Authors: Asbahr Wiebke , Lamparter Robin , Rauch Reinhard
Published: May 2024

For technical application with continuous operation of sorption-enhanced (SE) reactions, e.g., Fischer–Tropsch, a special reactor concept is required. SE processes are promising due to the negative effects of water on conversion and catalyst. The reactor concept of two interconnected slurry bubble columns combines the reaction with in situ water removal in the first, and sorbent regeneration in the second column with continuous exchange of slurry between the two. The liquid circulation rate (LCR) between the columns is studied in a cold flow model, measured by an ultrasonic sensor. The effects of different operating and geometric parameters, e.g., superficial gas velocity, liquid level and tube diameter on gas holdup and LCR are discussed and modelled via artificial intelligence methods, i.e., extremely randomized trees and neural networks. It was found that the LCR strongly depends on the gas holdup. The maximum of 4.28 L min−1 was reached with the highest exit, widest tube and highest superficial gas velocity of 0.15 m s−1. The influence of liquid level above the exit was marginal but water quality has to be considered. Both models offer predictions of the LCR with errors < 6%. With an extension of the models, particle circulation can be studied in the future.
Congenital Vascular Malformations in Children: From Historical Perspective to a Multidisciplinary Approach in the Modern Era—A Comprehensive Review
Authors: Bouwman Frédérique C. M. , Verhoeven Bas H. , Klein Willemijn M. , Schultze Kool Leo J. , de Blaauw Ivo
Published: May 2024

Congenital vascular malformations (CVMs) are the result of an aberrant development during embryogenesis. Although these lesions are present at birth, they are not always visible yet. Once symptomatic, patients suffer from pain, bleeding, ulcers, infections or lymphatic leakage, depending on the subtype of vessels involved. Treatment includes conservative management, surgery, sclerotherapy, embolization and pharmacological therapy. The clinical presentation varies widely and treatment can be challenging due to the rarity of the disease and potential difficulties of treatment. This review gives an overview of the historical developments in diagnosis and classification and exposes the key elements of innovations in the past decades on the identification of genetic mutations and personalized treatment. These advances in the field and a multidisciplinary approach are highly valuable in the optimization of clinical care aimed at both curing or stabilizing the CVM and pursuing physical and psychosocial wellbeing.
A CMOS 12-Bit 3MS/s Rad-Hard Digital-to-Analog Converter Based on a High-Linearity Resistor String Poly-Matrix
Authors: Calligaro Cristiano , Gatti Umberto
Published: May 2024

This work presents a rad-hard 12-bit 3 MS/s resistor string DAC for space applications. The converter has been developed using rad-hardened techniques both at architecture and layout levels starting from a conventional topology. The design considers the different effects of the radiation that could damage the circuits in space environments. The DAC has been developed and integrated a standard CMOS 0.13 μm technology by IHP, using RHBD techniques. Low Earth Orbit (LEO) requires a TID value of around 100 krad (Si), according to the expected length of the mission. The temperature range is between −55 °C and 125 °C. The DAC power budget is similar to that of terrestrial applications. The measured INL (Integral Non-Linearity) and DNL (Differential Non-Linearity) are better than 0.2 LSB, while the ENOB (Effective Number Of Bits) at a 3 MS/s clock exceeds 9.7 bits while loading a 10 pF capacitor. The DAC has been characterized under radiation, showing a fluctuation in the analog output lower than 2 LSB (mainly due to measurement uncertainty) up to 500 krad (Si). Power consumption shows a negligible increase, too. A 10-bit version of the same DAC as the downscaled 12-bit one has been developed as well.
A Novel Approach Using Reduced Graphene Oxide for the Detection of ALP and RUNX2 Osteogenic Biomarkers
Authors: Chiticaru Elena Alina , Ioniță Mariana
Published: May 2024

In this work, we propose a new technique involving the modification of commercial screen-printed carbon electrodes with electrochemically reduced graphene oxide to serve as the starting point of a future electrochemical biosensor for the detection of two osteogenic biomarkers: alkaline phosphatase (ALP) and Runt-related transcription factor 2 (RUNX2). The electrodes were characterized after each modification by cyclic voltammetry and electrochemical impedance spectroscopy, showing the appropriate electrochemical characteristics for each modification type. The results obtained from scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and contact angle measurements are well correlated with each other, demonstrating the successful modification of the electrodes with graphene oxide and its subsequent reduction. The bioreceptors were immobilized on the electrodes by physical adsorption, which was confirmed by electrochemical methods, structural characterization, and contact angle measurements. Finally, the functionalized electrodes were incubated with the specific target analytes and the detection relied on monitoring the electrochemical changes occurring after the hybridization process. Our results indicated that the pilot platform has the ability to detect the two biomarkers up to 1 nM, with increased sensitivity observed for RUNX2, suggesting that after further optimizations, it has a high potential to be employed as a future biosensor.
Thermal-Mechanical Coupling Analysis of Permeable Asphalt Pavements
Authors: Li Yuekun , Wang Xulong , Zhang Hailong , Li Zhenxia , Guo Tengteng
Published: May 2024

In order to clarify the mechanical response of permeable asphalt pavements under a temperature effect, the mechanical responses of different types of permeable asphalt pavements, which were based on a self-developed drainage SBS-modified asphalt mixture with fiber, were simulated using ANSYS finite element software (APDL 19.2). The influence of temperature and temperature change on the mechanical behavior of the permeable asphalt pavements was studied, and the mechanical responses of the pavements at different driving speeds was analyzed. The results show that the extreme values of surface deflection, compressive strain of the soil foundation top surface, and the shear stress and tensile stress of the upper-layer bottom of the three kinds of pavements under dynamic load were about 10% smaller than those under static loads, and the extreme values under different temperature conditions were 28%~50% larger than the values obtained without different temperature conditions. During the 12 h heating process, the mechanical indexes of the three types of pavements with axle loads were consistent with the change law of temperature, and the peak values of the mechanical indexes under dynamic loads were smaller than those under static loads. In addition, the mechanical indexes of the three types of pavements under dynamic loads had the same law of change with speed under the same conditions, and the values were less than the extreme values under static loads, but the degree of influence was different.
The “Forgotten” Hydroxyapatite Crystals in Regenerative Bone Tissue Engineering: A Critical Review
Authors: Tzavellas Anastasios-Nektarios , Katrilaka Chrysoula , Karipidou Niki , Kanari Magdalini , Pitou Maria , Koliakos Georgios , Cheva Angeliki , Choli-Papadopoulou Theodora , Aggeli Amalia , Tsiridis Eleftherios
Published: May 2024

Bone regeneration using Bone Morphogenetic Proteins (BMPs) alongside various engineered scaffolds has attracted considerable attention over the years. The field has seen extensive research in preclinical animal models, leading to the approval of two products and guiding the quest for new materials. Natural and synthetic polymers, ceramics, and composites have been used to fabricate the necessary porous 3D scaffolds and delivery systems for BMPs. Interestingly, all reported applications in the literature are triumphant. Evaluation of the results is typically based on histologic assessment after appropriate staining and radiological modalities, providing morphological identification of the newly formed bone and describing cells and the organic compound. Remarkably, while these evaluation methods illustrate mineralization, they are not capable of identifying hydroxyapatite crystals, the mineral component of the bone, which are crucial for its mechanical properties, structure, integrity, and long-term stability of regenerated bone tissue. This review aims to focus on the different scaffolds used in bone tissue engineering applications and underline the pressing need for techniques that could recognize the presence of hydroxyapatite crystals as well as their characteristics in bone tissue engineering, which will provide a more complete and comprehensive assessment of the successful results.
The Influence and Mechanism Analysis of the Longitudinal Magnetic Field on the Microstructure Evolution and Properties of AZ40 Welds
Authors: Wang Jianghui
Published: May 2024

This paper studied the effect of the longitudinal magnetic field (LMF) on the microstructure evolution and mechanical properties of AZ40 argon tungsten arc welding joints. Magnetic field-assisted argon tungsten arc welding technology was used to achieve butt welding of an AZ40 Mg alloy sheet with a thickness of 1.5 mm. The microstructure of the Mg alloy weld was studied by using metallographic microscopy and scanning electron microscopy. Mechanical performance of the Mg alloy weld was evaluated by using a hardness tester and universal tensile machine. The experimental results revealed that the average crystallite dimension of the weld zone of the Mg alloy joint reached 43 μm without an LMF. By introducing LMF-assisted technology, the weld structure was significantly refined and the average crystallite dimension of the weld seam was reduced by 39.5% to 26 μm with a coil current of 1.2 A. For the joint without magnetic field assistance, the optimum tensile strength of the AZ40 weldment was 225 MPa under a welding current of 80 A, and fracture occurred in the center of joint welding seam. Under an LMF coil current of 1.2 A, the joint strength increased from the initial 225 MPa to 254 MPa, and fracture occurred at the weld edge with obvious plastic fracture characteristics. It can be confirmed that the LMF-assisted welding process effectively improved the microstructure characteristics of the weld seam and strengthened the microhardness and mechanical performance of the AZ40 joint.
A Simplified Design Method for the Mechanical Stability of Slit-Shaped Additively Manufactured Reactor Modules
Authors: Metzger David F. , Klahn Christoph , Dittmeyer Roland
Published: May 2024

Equipment integrity is an essential aspect of process engineering. Design guidelines facilitate the design and production of safe-to-operate and economic devices. Thin-walled, slit-shaped modules form a subgroup of process engineering devices made via additive manufacturing (AM). Being subject to internal pressure, they have lacked design guidelines until now. We derived a user-centered calculation model for such modules with regular internal structures. It was validated with Finite Element Analysis (FEA) and practical pressure tests for which the modules were manufactured additively. The performance of the calculation could be confirmed, and a design graph was derived. Slit-shaped modules with appropriate internal structures can withstand high pressure at a minimum wall thickness, and they are efficiently fabricated. These structures, being pins, fins, lattice, or heat transfer enhancing fluid-guiding elements (FGEs), occupied approximately 10% of the modules’ internal volume.
Multiplex Assays in Allergy Diagnosis: Allergy Explorer 2 versus ImmunoCAP ISAC E112i
Authors: Nösslinger Hannes , Mair Ewald , Oostingh Gertie J. , Ahlgrimm-Siess Verena , Ringauf Anna , Lang Roland
Published: May 2024

ImmunoCAP ISAC E112i (ISAC) and Allergy Explorer 2 (ALEX2) detect specific immunoglobulin E (IgE) reactivity. Both multiplex assays contain molecular allergens and ALEX2 additionally includes allergen extracts and inhibitors that block the binding of IgE to cross-reacting carbohydrate determinants (CCDs). This study aimed to compare the performance of ISAC and ALEX2 by determining the IgE reactivity against allergen extracts and/or allergen components and by using qualitative, semiquantitative, and quantitative analyses of all comparable allergen components in sera from 216 participants recruited in South Tyrol/Italy. For extract sensitization in ALEX2, the analysis revealed negative corresponding allergen components in 18.4% and at least one positive corresponding allergen component in 81.6% of all cases. For ISAC, the corresponding results were 23.5% and 76.5% of cases, respectively. The ALEX2 CCD inhibitor eliminated CCD-positive signals detected by ISAC in 88.5% of cases. Based on sensitization values of 0.3–14.9 ISU or kUA/L, there was good agreement between ALEX2 and ISAC, although ALEX2 showed higher values than ISAC. The addition of allergen-extract tests in ALEX2 resulted in the detection of more sensitizations than with corresponding allergen components alone. In the range of <15 ISU or kUA/L, ALEX2 may be more effective in detecting sensitizations.
Exercise-Induced Electrocardiographic Changes in Healthy Young Males with Early Repolarization Pattern
Authors: Kocsis Loránd , Pap Zsuzsanna , László Szabolcs Attila , Gábor-Kelemen Hunor , Szabó István Adorján , Heidenhoffer Erhard , Frigy Attila
Published: May 2024

Background: Exercise-induced modifications in ECG parameters among individuals with an early repolarization pattern (ERP) have not been evaluated in detail. We aimed to assess this phenomenon, with potential associations with arrhythmogenesis. Methods: Twenty-three young, healthy males with ERP (ERP+) participated in this study, alongside a control group, which consisted of nineteen healthy males without ERP (ERP−). ECGs at baseline, at peak exercise (Bruce protocol), and during the recovery phase were analyzed and compared between the two groups. Results: The treadmill test demonstrated strong cardiovascular fitness, with similar chronotropic and pressor responses in both groups. In the baseline ECGs, the QRS complex and the QT interval were shorter in the ERP+ group. During exercise, the P-wave duration was significantly longer and the QRS was narrower in the ERP+ group. In the recovery phase, there was a longer P wave and a narrower QRS in the ERP+ group. During the treadmill test, the J wave disappeared or did not meet the criteria required for ERP diagnosis. Conclusions: The slowed intra-atrial conduction found during exercise could be predictive of atrial arrhythmogenesis in the setting of ERP. The disappearing of J waves during exercise, due to increased sympathetic activity, has potential clinical significance.
Machine Learning for Short-Term Mortality in Acute Decompensation of Liver Cirrhosis: Better than MELD Score
Authors: Salkić Nermin , Jovanović Predrag , Barišić Jaman Mislav , Selimović Nedim , Paštrović Frane , Grgurević Ivica
Published: May 2024

Prediction of short-term mortality in patients with acute decompensation of liver cirrhosis could be improved. We aimed to develop and validate two machine learning (ML) models for predicting 28-day and 90-day mortality in patients hospitalized with acute decompensated liver cirrhosis. We trained two artificial neural network (ANN)-based ML models using a training sample of 165 out of 290 (56.9%) patients, and then tested their predictive performance against Model of End-stage Liver Disease-Sodium (MELD-Na) and MELD 3.0 scores using a different validation sample of 125 out of 290 (43.1%) patients. The area under the ROC curve (AUC) for predicting 28-day mortality for the ML model was 0.811 (95%CI: 0.714- 0.907; p < 0.001), while the AUC for the MELD-Na score was 0.577 (95%CI: 0.435–0.720; p = 0.226) and for MELD 3.0 was 0.600 (95%CI: 0.462–0.739; p = 0.117). The area under the ROC curve (AUC) for predicting 90-day mortality for the ML model was 0.839 (95%CI: 0.776- 0.884; p < 0.001), while the AUC for the MELD-Na score was 0.682 (95%CI: 0.575–0.790; p = 0.002) and for MELD 3.0 was 0.703 (95%CI: 0.590–0.816; p < 0.001). Our study demonstrates that ML-based models for predicting short-term mortality in patients with acute decompensation of liver cirrhosis perform significantly better than MELD-Na and MELD 3.0 scores in a validation cohort.
JAKinhibs in Psoriatic Disease: Analysis of the Efficacy/Safety Profile in Daily Clinical Practice
Authors: Bizzarri Francesco , Ruiz-Villaverde Ricardo , Morales-Garrido Pilar , Ruiz-Carrascosa Jose Carlos , Cebolla-Verdugo Marta , Prados-Carmona Alvaro , Rodriguez-Troncoso Mar , Raya-Alvarez Enrique
Published: May 2024

Psoriatic disease (PsD) affects multiple clinical domains and causes a significant inflammatory burden in patients, requiring comprehensive evaluation and treatment. In recent years, new molecules such as JAK inhibitors (JAKinhibs) have been developed. These have very clear advantages: they act quickly, have a beneficial effect on pain, are well tolerated and the administration route is oral. Despite all this, there is still little scientific evidence in daily clinical practice. This observational, retrospective, single-center study was carried out in patients diagnosed with PsA in the last two years, who started treatment with Tofacitinib or Upadacitinib due to failure of a DMARD. The data of 32 patients were analyzed, and the majority of them (75%) started treatment with Tofacitinib. Most had moderate arthritis activity and mild psoriasis involvement according to activity indices. Both Tofacitinib and Upadacitinib demonstrated significant efficacy, with rapid and statistically significant improvement in joint and skin activity indices, C-reactive protein reduction, and objective measures of disease activity such as the number of painful and inflamed joints. Although there was some difference in the baseline characteristics of the cohort, treatment responses were comparable or even superior to those in the pivotal clinical trials. In addition, there was a low frequency of mild adverse events leading to treatment discontinuation and no serious adverse events. These findings emphasize the strong efficacy and tolerability of JAKinhibs in daily clinical practice, supporting their role as effective therapeutic options for patients with PsD.