bluuebunny/crossref_metadata_2025 huggingface dataset:
Bi-Objective Power Optimization of Radio Stripe Uplink Communications
Authors: Conceição Filipe , Gomes Marco , Silva Vitor , Dinis Rui , Henggeler Antunes Carlos
Published: March 2022

The radio stripe (RS) system is a practical implementation of cell-free mMIMO, in which a set of multi-antenna access points (APs) serves at the same time-frequency resources the user equipment (UE) in the network. The APs are sequentially connected in a stripe, sharing the same fronthaul link to the central processing unit. This work considers an uplink power optimization problem that aims to enhance the network spectral efficiency (SE) by considering two metrics—the max–min fairness and the max–sum rate. We employ a meta-heuristic based on the differential evolution algorithm to solve the bi-objective optimization problem. The SE performances of the full power along with the single-objective and multiple-objective scenarios are analyzed and compared for the optimal sequential linear processing detection scheme. The bi-objective approach is able to unveil the trade-offs to identify solution balancing the SE distribution resulting from the optimization of the max–min fairness and the max–sum rate objective functions.
Taxonomy and Stakeholder Risk Management in Integrated Projects of the European Green Deal
Authors: Tryhuba Anatoliy , Hutsol Taras , Kuboń Maciej , Tryhuba Inna , Komarnitskyi Serhii , Tabor Sylwester , Kwaśniewski Dariusz , Mudryk Krzysztof , Faichuk Oleksandr , Hohol Tetyana , Tomaszewska-Górecka Wioletta
Published: March 2022

The article analyzed the state of the global problem of ecological safety of the EU and argued the need to create a methodology for planning technologically integrated projects of the “European Green Deal” (TIP “EGD”) that accounts for the risks to the stakeholders, i.e., the state, project executors, resource suppliers, project managers, and clients. Each of these has an individual set of values that determines the project risk for them. Herein is proposed a taxonomy of three ranks of TIP “EGD” involving agricultural waste, determined by their characteristics, products, and requirements for the stakeholders. The authors point out the need to create tools for quantitative risk assessment for the stakeholders of TIP “EGD” involving agricultural waste and distinguish four groups of risk components with regard to the value of such projects. A model of value risk formation is presented that addresses the risk management of each of these stakeholder values. The need to develop tools (models, methods, and algorithms) for quantitative risk assessment of the values of each type of project is discussed. Regularities in the formation of stakeholder values, which were a foundation of the model of formation, are established.
Data-Driven Air-Fuel Path Control Design for Robust RCCI Engine Operation
Authors: Verhaegh Jan , Kupper Frank , Willems Frank
Published: March 2022

Reactivity controlled compression ignition (RCCI) is a highly efficient and clean combustion concept, which enables the use of a wide range of renewable fuels. Consequently, this promising dual fuel combustion concept is of great interest for realizing climate neutral future transport. RCCI is very sensitive for operating conditions and requires advanced control strategies to guarantee stable and safe operation. For real-world RCCI implementation, we face control challenges related to transients and varying ambient conditions. Currently, a multivariable air–fuel path controller that can guarantee robust RCCI engine operation is lacking. In this work, we present a RCCI engine controller, which combines static decoupling and a diagonal MIMO feedback controller. For control design, a frequency domain-based approach is presented, which explicitly deals with cylinder-to-cylinder variations using data-driven, cylinder-individual combustion models. This approach enables a systematic trade-off between fast and robust performance and gives clear design criteria for stable operation. The performance of the developed multivariable engine controller is demonstrated on a six-cylinder diesel-E85 RCCI engine. From experimental results, it is concluded that the RCCI engine controller accurately tracks the five desired combustion and air path parameters, simultaneously. For the studied transient cycle, this results in 12.8% reduction in NOx emissions and peak in-cylinder pressure rise rates are reduced by 3.8 bar/deg CA. Compared to open-loop control, the stable and safe operating range is increased from 25 °C up to 35 °C intake manifold temperature and maximal load range is increased by 14.7% up to BMEP = 14.8 bar.
Certainty-Equivalence-Based Sensorless Robust Sliding Mode Control for Maximum Power Extraction of an Uncertain Photovoltaic System
Authors: Alam Zaheer , Khan Qudrat , Khan Laiq , Ullah Safeer , Kirmani Syed Abdul Mannan , Algethami Abdullah A.
Published: March 2022

Photovoltaic (PV) arrays and their electronic converters are subject to various environmental disturbances and component-related faults that affect their normal operations and result in a considerable energy loss. Therefore, it is ever demanding to design such closed-loop operating algorithms that tolerate faults, present acceptable performance, and avoid wear and tear in the systems. In this work, the core objective is to extract maximum power from a PV array subject to environmental disturbances and plant uncertainties. The system is considered under input channel uncertainties (i.e., faults) along with variable resistive load and charging stations. A neuro-fuzzy network (NFN)-based reference voltage is generated to extract maximum power while considering variable temperature and irradiance as inputs. Furthermore, the estimated reference is tracked by the actual PV voltage under two types of controllers: certainty-equivalence-based robust sliding mode (CERSMC) and certainty-equivalence-based robust integral sliding mode (CERISMC). These strategies benefit from improving the robustness against faults (disturbances). The proposed methods use the inductor current, which is recovered via the velocity observer and the flatness property of nonlinear systems. The system’s stability is proven in the form of very appealing theorems. These claims are validated by the simulation results, which are carried out in a MATLAB environment.
Comparative Study of Inlet Structure and Obstacle Plate Designs Affecting the Temperature Stratification Characteristics
Authors: Trinuruk Piyatida , Jenyongsak Papangkorn , Wongwises Somchai
Published: March 2022

Temperature stratification between outgoing hot water and incoming cold water is a key factor in diminishing energy loss during the discharging process and maximizing the useful hot water delivered from the tank or enhancing the thermal efficiency of the heating device during the heating process. In this study, the inlet structure and the obstacle plate were designed and modified based on two main factors, the reduction of inlet water velocity and the stipulation of the water recirculation area, to develop temperature stratification through the computational fluid dynamics method. The simulation model’s accuracy was validated against the experimental results. The results showed that using the equalizer as an inlet pipe’s auxiliary device was the best approach for decreasing the inlet water velocity, which resulted in enhancing temperature stratification. The discharging efficiency improved from 77.3% for the original tank model to 86.1% for the tank with equalizer IV model, which meant an additional 45 L of useful hot water was gained from the good temperature stratification storage tank. The installation of the obstacle plate for controlling the turbulence zone could not improve temperature stratification significantly, which resulted in an increase in discharging efficiency by only 4% more than the original tank model.
Multifaceted Analysis of the Use of Catalytic Additives for Combustion with Hemp Pellets in a Low-Power Boiler
Authors: Knutel Bernard , Gaze Błażej , Wojtko Paulina , Dębowski Marcin , Bukowski Przemysław
Published: March 2022

This paper presents the results of a multifaceted analysis of the application of catalytic additives to hemp pellets’ combustion in a low-power boiler. The research concerns the effects of five catalytic additives applied inside the boiler’s combustion chamber—based on TiO2, MnO2, Cu(NO3)2 × 3H2O, H2PtCl6 solution, and 99.5% pure urea solution—on the quality of hemp pellets’ combustion process. For this purpose, technical and elemental analyses of the used fuel were performed. The chemical composition of exhaust gases (NOx, CO, SO2, and PM content) was also examined using an exhaust gas analyzer and a dust meter. The highest reductions in emissions of individual pollutants were for CO (−113%; combustion with Ad3), NOx (−66%; combustion with Ad 4), SO2 (−48%; combustion with Ad3), and PM (−78%; combustion with Ad1). The study also determined the amount of avoided costs due to the use of catalytic additives, as well as the annual prevented CO2 emissions to the atmosphere. Due to rising fuel and energy prices, this study could be helpful for biomass boiler owners who would like to burn locally available raw materials and increase the combustion process’ efficiency.
Revisiting the Performance of the Kernel-Driven BRDF Model Using Filtered High-Quality POLDER Observations
Authors: Li Hanliang , Yan Kai , Gao Si , Song Wanjuan , Mu Xihan
Published: March 2022

The Bidirectional Reflectance Distribution Function (BRDF) is usually used to describe the reflectance anisotropy of a non-Lambertian surface and estimate surface parameters. Among the BRDF models, the kernel-driven models have been extensively used due to their simple form and powerful fitting ability, and their reliability has been validated in some studies. However, existing validation efforts used in situ measurements or limited satellite data, which may be subject to inadequate observational conditions or quality uncertainties. A recently released high-quality BRDF database from Polarization and Directionality of the Earth’s Reflectances (POLDER) provides an opportunity to revisit the performance of the kernel-driven models. Therefore, in order to evaluate the fitting ability of the kernel-driven models under different observational conditions and explore their application direction in the future, we use the filtered high-quality BRDF database to evaluate the fitting ability of the kernel-driven model represented by the RossThick-LiSparseR (RTLSR) kernels in this paper. The results show that the RTLSR model performs well, which shows small fitting residuals under most observational conditions. However, the applicability of the RTLSR model performed differently across land cover types; the RTLSR model exhibited larger fitting residuals, especially over non-vegetated surfaces. Under different sun-sensor geometries, the fitting residuals show a strong positive correlation with the Solar Zenith Angle. The above two factors cause the RTLSR model to exhibit a poorer fitting ability at high latitudes. As an exploration, we designed a model combination strategy that combines the advantages of different models and achieved a better performance at high latitudes. We believe that this study provides a better understanding of the RTLSR model.
Tree-Ring Oxygen Isotope Variations in Subalpine Firs from the Western Himalaya Capture Spring Season Temperature Signals
Authors: Chinthala Bency David , Grießinger Jussi , Ranhotra Parminder Singh , Tomar Nidhi , Singh C. P. , Bräuning Achim
Published: March 2022

We analyzed the tree-rings δ18O of Abies spectabilis (fir) growing at the subalpine treeline ecotone in the Magguchatti valley. The valley is located in the Indian summer monsoon (ISM) dominated region of western Himalaya and also receives snow precipitation derived by westerly disturbances (WDs) during the winter months. The 60 year developed (1960–2019 CE) tree-ring δ18O chronology revealed a strong positive correlation with the temperature of late winter and spring months (February to April). Strong negative correlations are also apparent for snowcover, soilmoisture, and relative humidity for the same spring season. Our findings partly contrast the significant correlation results of tree-ring δ18O with summer precipitation and drought indices recorded from other summer monsoon-dominated regions in the Himalayas. The spatial correlation analyses with sea surface temperatures (SSTs) and climate parameters showed subdued signals of tropical Pacific at the site, but with a shift to more moisture influx from the Arabian Sea during the last two decades. Moreover, a significant negative correlation with North Atlantic Oscillation further justifies the strongly captured spring temperature and snowcover signals and the weak effect of summer precipitation in fir trees. A temperature rising trend during the latter half of the 20th century and the elevation effect are taken as important factors controlling the moisture source at the treeline ecotone zones.
Cloning and Expression Profiling of the Gene vasa during First Annual Gonadal Development of Cobia (Rachycentron canadum)
Authors: Ma Qian , Kuang Jiehua , Chen Gang , Zhang Jiandong , Huang Jiansheng , Mao Feifan , Zhou Qiling
Published: March 2022

The vasa gene is essential for germ cell development and gametogenesis both in vertebrates and in invertebrates. In the present study, vasa (Rcvasa) cDNA was cloned from cobia (Rachycentron canadum) using the RACE amplification method. We found that the full-length cDNA sequence of Rcvasa comprises 2571 bp, containing a 5′-UTR of 145 bp, a 3′-UTR of 341 bp, and an open reading frame (ORF) of 2085 bp, encoding a protein of 694 aa. The deduced amino acid sequence contains 8 conserved motifs of the DEAD-box protein family, 7 RGG repeats, and 10 RG repeats in the N-terminal region. Comparisons of the deduced amino acid sequence with those of other teleosts revealed the highest percentage identity (86.0%) with Seriola quinqueradiata. By using semiquantitative RT-PCR, Rcvasa appeared to be specifically expressed in the testis and ovary, among 13 tissues analyzed. In addition, annual changes in Rcvasa expression levels were examined in the gonads by quantitative real-time PCR (qRT-PCR). The expression of Rcvasa in the testis first increased significantly at 120 dph (stage II–III), then stabilized as the testis developed from 185 dph (stage III) to 360 dph (stage V). During the development of the ovary (stage I to II), the expression of Rcvasa first increased and reached the highest level at 210 dph (stage II), then decreased. Furthermore, the results of chromogenic in situ hybridization (CISH) revealed that Rcvasa mRNA was mainly expressed in germ cells and barely detected in somatic cells. In the testis, Rcvasa mRNA signal was concentrated in the periphery of spermatogonia, primary spermatocytes, and secondary spermatocytes and was significantly weaker in spermatids and spermatozoa. In the ovary, Rcvasa mRNA signal was uniformly distributed in the perinuclear cytoplasm and was intense in early primary oocytes (stage I and II). These findings could provide a reference for understanding the regulatory mechanisms of vasa expression during the development of germ cells in cobia.
What Economists Can Learn from “The Power of Us: Harnessing Our Shared Identities for Personal and Collective Success” by Jay J. Van Bavel and Dominick J. Packer
Authors: Grieco Daniela
Published: March 2022

Social identity theory has become increasingly important for economists. I discuss the contribution of Van Bavel and Packer’s “The Power of Us” in light of what economists (especially experimental ones) can learn from their research.
Polymorphism rs3742330 in microRNA Biogenesis Gene DICER1 Is Associated with Pseudoexfoliation Glaucoma in Saudi Cohort
Authors: Kondkar Altaf A. , Azad Taif A. , Sultan Tahira , Radhakrishnan Rakesh , Osman Essam A. , Almobarak Faisal A. , Lobo Glenn P. , Al-Obeidan Saleh A.
Published: March 2022

We investigated the association between DICER1 (rs3742330) and DROSHA (rs10719) polymorphisms and pseudoexfoliation glaucoma (PXG) and related clinical phenotypes in a Saudi cohort. In a retrospective case-control study, TaqMan real-time, PCR-based genotyping was performed in 340 participants with 246 controls and 94 PXG cases. The minor (G) allele frequency of rs3742330 in PXG (0.03) was significantly different from that in the controls (0.08) and protective against PXG (odds ratio (OR) = 0.38, 95% confidence interval (CI) = 0.16–0.92), p = 0.017). Similarly, the rs3742330 genotypes showed a significant protective association with PXG in dominant (p = 0.019, OR = 0.38, 95% CI = 0.15–0.92), over-dominant (p = 0.024, OR = 0.39, 95% CI = 0.16–0.95), and log-additive models (p = 0.017, OR = 0.38, 95% CI = 0.16–0.92). However, none remained significant after an adjustment for age, sex, and multiple testing. Rs10719 in DROSHA did not show any significant allelic or genotype association with PXG. However, a protective effect of the GA haplotype in DICER1 and DROSHA and PXG (p = 0.034) was observed. Both polymorphisms showed no significant effect on intraocular pressure and the cup–disk ratio. In conclusion, we report a significant genetic association between variant rs3742330 in DICER1, a gene involved in miRNA biogenesis, and PXG. Further investigation in a larger group of patients of different ethnicities and functional studies are warranted to replicate and validate its potential role in PXG.
Task and Ego Orientation in Sport Questionnaire (TEOSQ): Psychometric Properties in Its Digital Version
Authors: Morales-Sánchez Verónica , Pérez-Romero Nuria , Franquelo María Auxiliadora , Balaguer Isabel , Hernández-Mendo Antonio , Reigal Rafael E.
Published: March 2022

The Task and Ego Orientation in Sport Questionnaire’s (TEOSQ’s) psychometric properties have been explored in previous studies but never in its digital version, which facilitates data collection. The objective of this study was to analyze the psychometric properties of the online TEOSQ by MenPas 1.0. The sample was composed of 2320 users (58.4% women; 41.6% men), between 18 and 65 years old (M = 25.27, SD = 7.39). The methods used were Confirmatory Factor Analysis (CFA) and invariance analysis from the original 13-item model. The CFA was corrected for all samples (CFI = 0.92–0.94, TLI = 0.93–0.95, RMSEA = 0.07, SRMR = 0.06; df = 64; Bollen-Stine Bootstrap p = 0.02–0.07): general sample (χ2 = 720.72, χ2/df = 11.26), women (χ2 = 496.85, χ2/df = 7.76), men (χ2 = 321.67, χ2/df = 5.03), individual sports (χ2 = 525.26, χ2/df = 8.21), and team sports (χ2 = 306.01, χ2/df = 4.78). The results also indicate optimal adjustments for invariance: convergent, discriminant and composite reliability validity. The study evidence demonstrates the adequate psychometric properties of the digital version. To conclude, considering the results obtained, the model shows a good psychometric fit for the sample in its online format. The principal limitations were computer failures, as well as that the large part of the sample were users between 20 and 25 years old, so the heterogeneity should be improved. The practical implications of this study could improve the efficacy of data collection in sports motivation using the online TEOSQ.
Examining the Associations between Adverse Childhood Experiences, Health Risk Behaviours, and Psychological Well-Being in a Convenience Sample of Lithuanian University Students
Authors: Laurinaitytė Ilona , Assini-Meytin Luciana C. , Čunichina Ksenija
Published: March 2022

This study examines the associations between adverse childhood experiences (ACEs), health risk behaviours, and psychological well-being among Lithuanian university students. A cross-sectional survey was carried out with a convenience sample of 393 students (80.7% females and 19.3% males) recruited from mostly undergraduate courses (96.4%) in Lithuanian universities. Participants, aged 18–25 years (21.07 ± 1.53), completed a web-based survey in which they were asked to retrospectively self-report on ACEs while answering questions on health risk behaviours (e.g., smoking, substance use, riding a car with a drunk driver) and psychological well-being. Only 8.7% of the study sample experienced no ACEs, and almost half of the sample (48.9%) experienced ≥4 ACEs. Findings from adjusted models showed that, compared with students with no ACEs, those who experienced ≥4 ACEs had higher odds of lifetime illicit drug use (AOR = 2.73, p < 0.05), riding with a drunk driver (AOR = 2.44, p < 0.05), suicidal ideation before age 18 (AOR = 28.49, p < 0.01) and in the past 12 months (AOR = 5.39, p < 0.01). An increased number of ACEs was also associated with lower psychological well-being (B = −3.94, p < 0.001). Findings from this study have implications for mental health professionals as well as university administrators, as students with a higher number of traumatic experiences may require greater levels of support and services.
COVID-19 Pandemic Impacts on Children with Developmental Disabilities: Service Disruption, Transition to Telehealth, and Child Wellbeing
Authors: Zhang Saijun , Hao Ying , Feng Yali , Lee Na Youn
Published: March 2022

The COVID-19 pandemic has resulted in substantial service disruption and transition from in-person services to telehealth for children with developmental disabilities. However, there is limited knowledge about the specific dimensions and consequences of the disruption and transition. This study aims to examine the extent of service disruption and transition, the experiences of client children and their caregivers with telehealth vis-à-vis in-person services, and the impacts of the disruption and transition on child wellbeing. The cross-sectional study collected data from parents of children with developmental disabilities using an online survey. McNemar’s tests were used to compare service changes before and after the pandemic outbreak, and multivariate analyses were used to examine how service changes were associated with child wellbeing. Results show that more than two-thirds of the children experienced reduction in service amount, and one-third lost services for more than two months in about five months into the pandemic. While telehealth had comparable features relative to in-person services, it had lower ratings with respect to diagnostic accuracy, treatment effectiveness, and rapport building. Service disruption/transition and social isolation were associated with behavioral and emotional deterioration in children. However, child and family stress may have confounded these adverse effects. We concluded that the magnitude of service disruption and transition was large in the first half year after the pandemic outbreak, and the amount and duration of service loss varied substantially across clients. Diagnostic accuracy, treatment efficacy, and rapport building were areas in which parents had major concerns toward telehealth relative to in-person services. However, such drawbacks may partially be due to the limited logistics in telehealth implementation during the pandemic. Service disruption and transition seemed to contribute to family stress, which played a direct role in eroding child wellbeing. Implications of these findings for future research and practices are discussed.
Inconsistent Country-Wide Reporting of Adverse Drug Reactions to Antimicrobials in Sierra Leone (2017–2021): A Wake-Up Call to Improve Reporting
Authors: Thomas Fawzi , Abiri Onome T. , Komeh James P. , Conteh Thomas A. , Bah Abdulai Jawo , Kanu Joseph Sam , Terry Robert , Abrahamyan Arpine , Thekkur Pruthu , Zachariah Rony
Published: March 2022

Background: Monitoring of adverse drug reactions (ADRs) to antimicrobials is important, as they can cause life-threatening illness, permanent disabilities, and death. We assessed country-wide ADR reporting on antimicrobials and their outcomes. Methods: A cross-sectional study was conducted using individual case safety reports (ICSRs) entered into the national pharmacovigilance database (VigiFlow) during 2017–2021. Results: Of 566 ICSRs, inconsistent reporting was seen, with the highest reporting in 2017 and 2019 (mass drug campaigns for deworming), zero reporting in 2018 (reasons unknown), and only a handful in 2020 and 2021 (since COVID-19). Of 566 ICSRs, 90% were for antiparasitics (actively reported during mass campaigns), while the rest (passive reporting from health facilities) included 8% antibiotics, 7% antivirals, and 0.2% antifungals. In total, 90% of the reports took >30 days to be entered (median = 165; range 2–420 days), while 44% had <75% of all variables filled in (desired target = 100%). There were 10 serious ADRs, 18 drug withdrawals, and 60% of ADRs affected the gastrointestinal system. The patient outcomes (N-566) were: recovered (59.5%), recovering (35.5%), not recovered (1.4%), death (0.2%), and unknown (3.4%). There was no final ascertainment of ‘recovering’ outcomes. Conclusions: ADR reporting is inconsistent, with delays and incomplete data. This is a wake-up call for introducing active reporting and setting performance targets.
Falsified Medicines Directive in a Secondary Care Environment—Impact on Supply Chain
Authors: Merks Piotr , Religioni Urszula , Pinto de Castro Nuno , Augustynowicz Anna , Plagens-Rotman Katarzyna , Brindley David , Kowalczuk Anna , Kaźmierczak Justyna , Neumann-Podczaska Agnieszka , Blicharska Eliza , Sola Katarina Fehir , Hug Martin J. , Gajewski Klaudiusz , Piątkiewicz Paweł
Published: March 2022

The Falsified Medicines Directive (FMD) and the Delegated Regulation (DR) impact the pharmaceutical supply chain. Ahead of the deadline for implementation, in February 2019, every entity of the supply chain had to adapt its operations to the regulatory requirements to be compliant with the directive. This paper analyzes the supply chain of a hospital pharmacy and the impact of the FMD implementation. Furthermore, a cost analysis was performed demonstrating that the FMD increases expenditure in the secondary care environment dispensing operations. Governments should be aware that this regulation will certainly impact public healthcare institutions in the long term.
The Impact of Functional Bars and Adapted Physical Activity on Quality of Life in Chronic Kidney Disease: A Pilot Study
Authors: Grazioli Elisa , Tranchita Eliana , Marrone Giulia , Urciuoli Silvia , Di Lauro Manuela , Cerulli Claudia , Piacentini Nicolò , Murri Arianna , Celotto Roberto , Romani Annalisa , Parisi Attilio , Di Daniele Nicola , Noce Annalisa
Published: March 2022

Chronic kidney disease (CKD) represents a public health problem because it is characterized by several comorbidities, including uremic sarcopenia (US), and a poor quality of life. Currently, there are no standardized treatments available to counteract the onset of US but only some possible therapeutic approaches to slow its progression. The aim of this pilot study is to collect descriptive data in order to design a clinical trial based on the power analysis and simple size. The purpose of this pilot study was to evaluate the possible beneficial action induced by the functional anti-inflammatory and antioxidant bars in combination with the adapted physical activity (APA), on the onset and progression of US and other related-CKD comorbidities. We enrolled 21 CKD patients under conservative therapy, divided into four groups: (A) the physical exercise program (PEP), three times a week, in combination with the daily consumption of the two functional bars group; (B) the PEP group; (C) the daily consumption of the two functional bars group; (D) the control group. The duration of the study protocol was 12 weeks. We observed an improvement trend of body composition, blood pressure levels, lipid metabolism, and functional test in A and B groups. These preliminary data would seem to confirm the effectiveness of APA and to demonstrate the additive role of the natural bioactive compound’s assumption in countering US and other CKD comorbidities.
Risk Exposure to Legionella pneumophila during Showering: The Difference between a Classical and a Water Saving Shower System
Authors: Niculita-Hirzel Hélène , Vanhove Audrey S. , Leclerc Lara , Girardot Françoise , Pourchez Jérémie , Allegra Séverine
Published: March 2022

The increase in legionellosis incidence in the general population in recent years calls for a better characterization of the sources of infection, such as showering. Water-efficient shower systems that use water-atomizing technology have been shown to emit slightly more inhalable particles in the range of bacterial sizes than the traditional systems; however, the actual rate of bacterial emission remains poorly documented. The aim of this study was to assess the aerosolisation rate of the opportunistic water pathogen Legionella pneumophila during showering with one shower system representative of each technology. To achieve this objective, we performed controlled experiments inside a glove box and determined the emitted dose and viability of airborne Legionella. The bioaerosols were sampled with a Coriolis® Delta air sampler and the total number of viable (cultivable and noncultivable) Legionella was determined by flow cytometry and culture. We found that the rate of viable and cultivable Legionella aerosolized from the water jet was similar between the two showerheads: the viable fraction represents 0.02% of the overall bacteria present in water, while the cultivable fraction corresponds to only 0.0005%. The two showerhead models emitted a similar ratio of airborne Legionella viable and cultivable per volume of water used. Therefore, the risk of exposure to Legionella is not expected to increase significantly with the new generation of water-efficient showerheads.
A Roadmap toward Achieving Sustainable Environment: Evaluating the Impact of Technological Innovation and Globalization on Load Capacity Factor
Authors: Awosusi Abraham Ayobamiji , Kutlay Kaan , Altuntaş Mehmet , Khodjiev Bakhtiyor , Agyekum Ephraim Bonah , Shouran Mokhtar , Elgbaily Mohamed , Kamel Salah
Published: March 2022

Technological innovations have been a matter of contention, and their environmental consequences remain unresolved. Moreover, studies have extensively evaluated environmental challenges using metrics such as nitrogen oxide emissions, sulfur dioxide, carbon emissions, and ecological footprint. The environment has the supply and demand aspect, which is not a component of any of these indicators. By measuring biocapacity and ecological footprint, the load capacity factor follows a certain ecological threshold, allowing for a thorough study on environmental deterioration. With the reduction in load capacity factor, the environmental deterioration increases. In the context of the environment, the interaction between technological innovation and load capacity covers the demand and supply side of the environment. In light of this, employing the dataset ranging from 1980 to 2017 for the case of South Africa, the bound cointegration test in conjunction with the critical value of Kripfganz and Schneider showed cointegration in the model. The study also employed the ARDL, whose outcome revealed that nonrenewable energy usage and economic growth contribute to environmental deterioration, whereas technological innovation and globalization improve the quality of the environment. This study validated the hypothesis of the environmental Kuznets curve for South Africa, as the short-term coefficient value was lower than the long-term elasticity. Furthermore, using the frequency-domain causality test revealed that globalization and economic growth predict load capacity in the long term, and nonrenewable energy predicts load capacity factors in the long and medium term. In addition, technological innovation predicts load capacity factors in the short and long term. Based on the findings, we propose that policymakers should focus their efforts on increasing funding for the research and development of green technologies.
Seeking Health Information: A Qualitative Study of the Experiences of Women of Refugee Background from Myanmar in Perth, Western Australia
Authors: Griffin Georgia , Nau S. Zaung , Ali Mohammed , Riggs Elisha , Dantas Jaya A. R.
Published: March 2022

Women of refugee background are subject to significant health inequity. Access to health information and a good level of health literacy are integral components to manage one’s health needs. The aim of this study isto understand the experiences of women of refugee background from Myanmar seeking and accessing health information. Semi-structured interviews were conducted with 14 women of refugee background from Myanmar resettled in Western Australia. Interpretative phenomenological analysis underpinned the study and was conducted on the interview data. Three superordinate themes and nine subordinate themes emerged from the analysis: (1) Seeking health information (Motivation and Sources), (2) Facilitators and Barriers (Communication, Navigating the system and Community) and (3) Seeking health information in the context of past experiences (Health information as a by-product of healthcare, Health professionals’ provision of health information, Accessibility of healthcare and Expectations on resettlement). These themes provide insight into the challenges of accessing understandable and actionable health information and of promoting the health literacy of women of refugee background from Myanmar. Co-designed community-based and health service interventions should be trialled, including trauma-informed training for health professionals, health information apps and community health promotion programs. Community engagement, participation and evaluation are critical for determining the effective interventions to address the inequalities experienced by this population.
Efficacy of the Virtual Reality Intervention VR FestLab on Alcohol Refusal Self-Efficacy: A Cluster-Randomized Controlled Trial
Authors: Guldager Julie Dalgaard , Kjær Satayesh Lavasani , Grittner Ulrike , Stock Christiane
Published: March 2022

It is currently unknown whether a virtual social environment can support young people in building their skills to overcome peer pressure when offered alcohol. This study evaluated the efficacy of the newly developed virtual reality simulation game VR FestLab on the refusal self-efficacy regarding social pressures to drink of Danish male and female students aged 15–18. VR FestLab features a party setting where adolescents can “steer” their own party experience. Eleven schools were included in a cluster-randomized controlled trial and allocated to either the intervention (n = 181) or the active control group (n = 191). Students in intervention schools played VR FestLab, while those in the control group played the VR game Oculus Quest—First Steps. The primary outcome measure was the social pressure subscale of the drinking refusal self-efficacy scale (DRSEQ-RA). The intervention effects were measured immediately after the intervention/control session (T1) and after a 6-week follow-up (T2). Data were examined using linear mixed regression models. Our study did not demonstrate a significant effect of drinking refusal self-efficacy at T1. For all secondary outcomes, we observed no substantial differences between the intervention and control groups. This study provides new insights into the feasibility and effectiveness of an innovative virtual reality alcohol prevention tool. VR FestLab can be an innovative and promising contribution to complement existing school-based alcohol prevention, but more research is needed to improve its effectiveness.
High Doses of Pesticides Induce mtDNA Damage in Intact Mitochondria of Potato In Vitro and Do Not Impact on mtDNA Integrity of Mitochondria of Shoots and Tubers under In Vivo Exposure
Authors: Alimova Alina A. , Sitnikov Vadim V. , Pogorelov Daniil I. , Boyko Olga N. , Vitkalova Inna Y. , Gureev Artem P. , Popov Vasily N.
Published: March 2022

It is well known that pesticides are toxic for mitochondria of animals. The effect of pesticides on plant mitochondria has not been widely studied. The goal of this research is to study the impact of metribuzin and imidacloprid on the amount of damage in the mtDNA of potato (Solanum tuberosum L.) in various conditions. We developed a set of primers to estimate mtDNA damage for the fragments in three chromosomes of potato mitogenome. We showed that both metribuzin and imidacloprid considerably damage mtDNA in vitro. Imidacloprid reduces the rate of seed germination, but does not impact the rate of the growth and number of mtDNA damage in the potato shoots. Field experiments show that pesticide exposure does not induce change in aconitate hydratase activity, and can cause a decrease in the rate of H2O2 production. We can assume that the mechanism of pesticide-induced mtDNA damage in vitro is not associated with H2O2 production, and pesticides as electrophilic substances directly interact with mtDNA. The effect of pesticides on the integrity of mtDNA in green parts of plants and in crop tubers is insignificant. In general, plant mtDNA is resistant to pesticide exposure in vivo, probably due to the presence of non-coupled respiratory systems in plant mitochondria.
Putrescine: A Key Metabolite Involved in Plant Development, Tolerance and Resistance Responses to Stress
Authors: González-Hernández Ana Isabel , Scalschi Loredana , Vicedo Begonya , Marcos-Barbero Emilio Luis , Morcuende Rosa , Camañes Gemma
Published: March 2022

Putrescine (Put) is the starting point of the polyamines (PAs) pathway and the most common PA in higher plants. It is synthesized by two main pathways (from ornithine and arginine), but recently a third pathway from citrulline was reported in sesame plants. There is strong evidence that Put may play a crucial role not only in plant growth and development but also in the tolerance responses to the major stresses affecting crop production. The main strategies to investigate the involvement of PA in plant systems are based on the application of competitive inhibitors, exogenous PAs treatments, and the most efficient approaches based on mutant and transgenic plants. Thus, in this article, the recent advances in understanding the role of this metabolite in plant growth promotion and protection against abiotic and biotic stresses will be discussed to provide an overview for future research.
Photobiomodulation Therapy and the Glymphatic System: Promising Applications for Augmenting the Brain Lymphatic Drainage System
Authors: Salehpour Farzad , Khademi Mahsa , Bragin Denis E. , DiDuro Joseph O.
Published: March 2022

The glymphatic system is a glial-dependent waste clearance pathway in the central nervous system, devoted to drain away waste metabolic products and soluble proteins such as amyloid-beta. An impaired brain glymphatic system can increase the incidence of neurovascular, neuroinflammatory, and neurodegenerative diseases. Photobiomodulation (PBM) therapy can serve as a non-invasive neuroprotective strategy for maintaining and optimizing effective brain waste clearance. In this review, we discuss the crucial role of the glymphatic drainage system in removing toxins and waste metabolites from the brain. We review recent animal research on the neurotherapeutic benefits of PBM therapy on glymphatic drainage and clearance. We also highlight cellular mechanisms of PBM on the cerebral glymphatic system. Animal research has shed light on the beneficial effects of PBM on the cerebral drainage system through the clearance of amyloid-beta via meningeal lymphatic vessels. Finally, PBM-mediated increase in the blood–brain barrier permeability with a subsequent rise in Aβ clearance from PBM-induced relaxation of lymphatic vessels via a vasodilation process will be discussed. We conclude that PBM promotion of cranial and extracranial lymphatic system function might be a promising strategy for the treatment of brain diseases associated with cerebrospinal fluid outflow abnormality.
Improving Photostability of Photosystem I-Based Nanodevice by Plasmonic Interactions with Planar Silver Nanostructures
Authors: Szalkowski Marcin , Kowalska Dorota , Olmos Julian David Janna , Kargul Joanna , Maćkowski Sebastian
Published: March 2022

One of the crucial challenges for science is the development of alternative pollution-free and renewable energy sources. One of the most promising inexhaustible sources of energy is solar energy, and in this field, solar fuel cells employing naturally evolved solar energy converting biocomplexes—photosynthetic reaction centers, such as photosystem I—are of growing interest due to their highly efficient photo-powered operation, resulting in the production of chemical potential, enabling synthesis of simple fuels. However, application of the biomolecules in such a context is strongly limited by the progressing photobleaching thereof during illumination. In the current work, we investigated the excitation wavelength dependence of the photosystem I photodamage dynamics. Moreover, we aimed to correlate the PSI–LHCI photostability dependence on the excitation wavelength with significant (ca. 50-fold) plasmonic enhancement of fluorescence due to the utilization of planar metallic nanostructure as a substrate. Finally, we present a rational approach for the significant improvement in the photostability of PSI in anoxic conditions. We find that photobleaching rates for 5 min long blue excitation are reduced from nearly 100% to 20% and 70% for substrates of bare glass and plasmonically active substrate, respectively. Our results pave promising ways for optimization of the biomimetic solar fuel cells due to synergy of the plasmon-induced absorption enhancement together with improved photostability of the molecular machinery of the solar-to-fuel conversion.
Hypothyroidism and Diabetes-Related Dementia: Focused on Neuronal Dysfunction, Insulin Resistance, and Dyslipidemia
Authors: Kim Hee Kyung , Song Juhyun
Published: March 2022

The incidence of dementia is steadily increasing worldwide. The risk factors for dementia are diverse, and include genetic background, environmental factors, sex differences, and vascular abnormalities. Among the subtypes of dementia, diabetes-related dementia is emerging as a complex type of dementia related to metabolic imbalance, due to the increase in the number of patients with metabolic syndrome and dementia worldwide. Thyroid hormones are considered metabolic regulatory hormones and affect various diseases, such as liver failure, obesity, and dementia. Thyroid dysregulation affects various cellular mechanisms and is linked to multiple disease pathologies. In particular, hypothyroidism is considered a critical cause for various neurological problems—such as metabolic disease, depressive symptoms, and dementia—in the central nervous system. Recent studies have demonstrated the relationship between hypothyroidism and brain insulin resistance and dyslipidemia, leading to diabetes-related dementia. Therefore, we reviewed the relationship between hypothyroidism and diabetes-related dementia, with a focus on major features of diabetes-related dementia such as insulin resistance, neuronal dysfunction, and dyslipidemia.
Alteration of Oral and Perioral Soft Tissue in Mice following Incisor Tooth Extraction
Authors: Takagi Takahiro , Yamamoto Masahito , Sugano Aki , Kanehira Chiemi , Kitamura Kei , Katayama Masateru , Sakai Katsuhiko , Sato Masaki , Abe Shinichi
Published: March 2022

Oral and perioral soft tissues cooperate with other oral and pharyngeal organs to facilitate mastication and swallowing. It is essential for these tissues to maintain their morphology for efficient function. Recently, it was reported that the morphology of oral and perioral soft tissue can be altered by aging or orthodontic treatment. However, it remains unclear whether tooth loss can alter these tissues’ morphology. This study examined whether tooth loss could alter lip morphology. First, an analysis of human anatomy suggested that tooth loss altered lip morphology. Next, a murine model of tooth loss was established by extracting an incisor; micro-computed tomography revealed that a new bone replaced the extraction socket. Body weight was significantly lower in the tooth loss (UH) group than in the non-extraction control (NH) group. The upper lip showed a greater degree of morphological variation in the UH group. Proteomic analysis and immunohistochemical staining of the upper lip illustrated that S100A8/9 expression was higher in the UH group, suggesting that tooth loss induced lip inflammation. Finally, soft-diet feeding improved lip deformity associated with tooth loss, but not inflammation. Therefore, soft-diet feeding is essential for preventing lip morphological changes after tooth loss.
Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection
Authors: Olmos-Ortiz Andrea , Hernández-Pérez Mayra , Flores-Espinosa Pilar , Sedano Gabriela , Helguera-Repetto Addy Cecilia , Villavicencio-Carrisoza Óscar , Valdespino-Vazquez María Yolotzin , Flores-Pliego Arturo , Irles Claudine , Rivas-Santiago Bruno , Moreno-Verduzco Elsa Romelia , Díaz Lorenza , Zaga-Clavellina Verónica
Published: March 2022

An infectious process into the uterine cavity represents a major endangered condition that compromises the immune privilege of the maternal–fetal unit and increases the risk for preterm birth (PTB) and premature rupture of membranes (PROM). Fetal membranes are active secretors of antimicrobial peptides (AMP), which limit bacterial growth, such as Escherichia coli. Nevertheless, the antibacterial responses displayed by chorioamniotic membranes against a choriodecidual E. coli infection have been briefly studied. The objective of this research was to characterize the profile of synthesis, activity, and spatial distribution of a broad panel of AMPs produced by fetal membranes in response to E. coli choriodecidual infection. Term human chorioamniotic membranes were mounted in a two independent compartment model in which the choriodecidual region was infected with live E. coli (1 × 105 CFU/mL). Amnion and choriodecidual AMP tissue levels and TNF-α and IL-1β secretion were measured by the enzyme-linked immunosorbent assay. The passage of bacterium through fetal membranes and their effect on structural continuity was followed for 24 h. Our results showed that E. coli infection caused a progressive mechanical disruption of the chorioamniotic membranes and an activated inflammatory environment. After the challenge, the amnion quickly (2–4 h) induced production of human beta defensins (HBD)-1, HBD-2, and LL-37. Afterwards (8–24 h), the amnion significantly produced HBD-1, HBD-2, HNP-1-3, S100A7, sPLA2, and elafin, whereas the choriodecidua induced LL-37 synthesis. Therefore, we noticed a temporal- and tissue-specific pattern regulation of the synthesis of AMPs by infected fetal membranes. However, fetal membranes were not able to contain the collagen degradation or the bacterial growth and migration despite the battery of produced AMPs, which deeply increases the risk for PTB and PROM. The mixture of recombinant HBDs at low concentrations resulted in increased bactericidal activity compared to each HBD alone in vitro, encouraging further research to study AMP combinations that may offer synergy to control drug-resistant infections in the perinatal period.