bluuebunny/crossref_metadata_2025 huggingface dataset:
Diagnosis, Mechanisms, and Differentiation of Inflammatory Diseases of the Outer Retina: Photoreceptoritis versus Choriocapillaritis; A Multimodal Imaging Perspective
Authors: Papasavvas Ioannis , Mantovani Alessandro , Herbort Carl
Published: September 2022

Background and aim: Inflammatory diseases that affect the outer retina do so by different mechanisms. Some of them result from the direct, primary involvement of the outer retina (primary photoreceptoritis) such as acute zonal outer occult retinopathy (AZOOR). Others affect the photoreceptors secondarily due to the inflammatory involvement of the choriocapillaris. This results in choriocapillaris non-perfusion that damages the photoreceptors due to the ensuing ischaemia, a mechanism characterising primary inflammatory choriocapillaropathies (PICCPs) such as multiple evanescent white dot syndrome (MEWDS), idiopathic multifocal choroiditis (MFC), and others. Thanks to multimodal imaging (MMI), it is now possible to differentiate between these two mechanisms of outer retinal damage. The aim of this study is to determine the MMI characteristics that allow us to differentiate primary photoreceptoritis, including AZOOR, from PICCPs such as MEWDS and MFC. Methods: A series of eight PICCPs cases (five typical MEWDS and three typical active MFC cases) and four typical primary photoreceptoritis/AZOOR cases (five eyes) that had undergone complete MMI investigation, including fundus photography (FP), blue light fundus autofluorescence (BL-FAF), spectral domain optical coherence tomography (SD-OCT), OCT angiography (OCT-A, when available), fluorescein angiography (FA), and indocyanine green angiography (ICGA) were analysed, pointing out the differences that allow us to distinguish primary photoreceptoritis from PICCPs. Results: All primary photoreceptoritis/AZOOR cases showed (1) faint fundus pallor around the fovea, (2) BL-FAF hyperautofluorescence, (3) loss of photoreceptor outer segments (PROS) on SD-OCT, (4) absence of choriocapillary drop-out on OCT-A, (5) normal FA or faint FA hyperfluorescence, and (6) conserved ICGA fluorescence/no hypofluorescent areas; (1), (2), (3), and (5) indicated loss of photoreceptor outer segments, and (4) and (6) indicated conserved choriocapillaris circulation. For PICCPs, (a) fundus showed discreet white dots or none (in MEWDS) and punched-out scars in MFC, (b) BL-FAF hyperautofluorescence, (c) loss of PROS on SD-OCT, (d) FA faint hyperfluorescence in MEWDS, also minimal in active MFC lesions (e) in all cases ICGA hypofluorescent areas; (b) and (c) indicating loss of PROS, and (e) indicating choriocapillaris non-perfusion in all cases. The OCT-A did not show consistent findings with faint or no capillary drop-out in MEWDS and MFC. Conclusions: MMI combining the SD-OCT and BL-FAF clearly showed loss of PROS in both groups, while the ICGA determined whether this was due to choriocapillaris non-perfusion in PICCPs or whether the choriocapillaris was intact in case of primary photoreceptoritis. The FA and OCT-A were found to be less useful and/or less sensitive for the appraisal of both these entities.
Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)
Authors: Piccirilli Alessandra , Cherubini Sabrina , Brisdelli Fabrizia , Fazii Paolo , Stanziale Andrea , Di Valerio Susanna , Chiavaroli Valentina , Principe Luigi , Perilli Mariagrazia
Published: September 2022

The whole-genome sequencing (WGS) of eighteen S. marcescens clinical strains isolated from 18 newborns hospitalized in the Neonatal Intensive Care Unit (NICU) at Pescara Public Hospital, Italy, was compared with that of S. marcescens isolated from cradles surfaces in the same ward. The identical antibiotic resistance genes (ARGs) and virulence factors were found in both clinical and environmental S. marcescens strains. The aac(6′)-Ic, tetA(41), blaSRT-3, adeFGH, rsmA, and PBP3 (D350N) genes were identified in all strains. The SRT-3 enzyme, which exhibited 10 amino acid substitutions with respect to SST-1, the constitutive AmpC β-lactamase in S. marcescens, was partially purified and tested against some β-lactams. It showed a good activity against cefazolin. Both clinical and environmental S. marcescens strains exhibited susceptibility to all antibiotics tested, with the exception of amoxicillin/clavulanate.
A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome
Authors: Xu Jiyu , Zheng Shuxin , Li Mimi , Liu Xiaoyan , Sun Haidan , Guo Zhengguang , Wei Jing , Jia Lulu , Sun Wei
Published: September 2022

Profiling bodily fluids is crucial for monitoring and discovering metabolic markers of disease. In this study, a comprehensive analysis approach based on 1D-LC-MS/MS and 2D-LC-MS/MS was applied to profile normal human urine metabolites from 348 children and 315 adults. A total of 2357 metabolites were identified, including 1831 endogenous metabolites and 526 exogenous ones. In total, 1005 metabolites were identified in urine for the first time. The urinary metabolites were mainly involved in amino acid metabolism, small molecule biochemistry, lipid metabolism and cellular compromise. The comparison of adult’s and children’s urine metabolomes showed adults urine had more metabolites involved in immune response than children’s, but the function of binding of melatonin, which belongs to the endocrine system, showed a higher expression in children. The urine metabolites detected by the 1D-LC-MS/MS method were mainly related to amino acid metabolism and lipid metabolism, and the 2D-LC-MS/MS method not only explored metabolites from 1D-LC-MS/MS but also metabolites related to cell signaling, cell function and maintenance, etc. Our analysis comprehensively profiled and functionally annotated the metabolome of normal human urine, which would benefit the application of urinary metabolome to clinical research.
Factors Associated with the Severity of Pregnancy-Related Hypertensive Disorder: Significance of Clinical, Laboratory, and Histopathological Features
Authors: An Hyo Jung , Song Dae Hyun , Kim Yu-Min , Jo Hyen Chul , Baek Jong Chul , Kim Hyoeun , Yang Jusuk , Park Ji Eun
Published: September 2022

The purpose of this paper is to evaluate the association of maternal clinical and laboratory features and placental histopathological changes with disease severity in pregnancy-related hypertensive disorders. From January 2021 to December 2021, clinical and laboratory data at the time of delivery and histopathological features of the placenta were collected from pregnant women with pregnancy-related hypertensive disorders at a single institution. The women were classified according to the pregnancy-related hypertensive disorder clinical severity, and each variable was compared accordingly. Gestational age-matched normotensive groups were also compared. Univariate and multivariate regression analyses were used to identify factors influencing pregnancy-related hypertensive disorder severity. Fifty-eight pregnancies were analyzed. Maternal albumin levels before delivery (beta coefficient −0.83, p = 0.043) and increased placental syncytial knots (beta coefficient 0.71, p = 0.026) are important parameters that are closely related to disease severity in women with pregnancy-related hypertensive disorders. The combination of albumin, PAPP-A, total bilirubin, and eGFR levels appears to be optimal for predicting pregnancy-related hypertensive disorder severity.
Does Country Risk Influence Foreign Direct Investment Inflows? A Case of the Visegrád Four
Authors: Hassan Adewale Samuel
Published: September 2022

The determinants of FDI inflows have been a subject of unremitting debate in the economic literature over the years. However, the role of country risk has received inadequate attention, especially in the context of the Visegrád countries, which comprise the Czech Republic, Hungary, Poland and Slovakia. Hence, this study examined whether country risk matters for FDI inflows into the Visegrád Four for the period 1991–2020. This study accounted for cross-sectional dependency, structural breaks and heterogeneous slopes in the panel of the four countries by employing the dynamic common correlated effect estimator. Additionally, country-wise fully modified least-squares regression was conducted for each country to test the robustness of the estimates. The empirical results revealed that country risk matters for the FDI inflows into the Visegrád countries, as it has a negative effect on the FDI inflows. Furthermore, both the overall panel and country-wise regressions established that economic and political risks are essential determinants of the FDI inflows, as both have a negative relationship with the FDI inflows. However, financial risk had weak and mixed impacts on the FDI inflows in the overall panel and country-wise regressions, respectively. These research outcomes highlight the need for appropriate macroeconomic and government authorities in the Visegrád economies to enhance the market capabilities of their economies by improving and upholding the social, institutional, corporate and macroeconomic structures, and as a way of achieving better country risk attributes.
6G-Enabled Smart Agriculture: A Review and Prospect
Authors: Zhang Fan , Zhang Yu , Lu Weidang , Gao Yuan , Gong Yi , Cao Jiang
Published: September 2022

As human society develops, the population is growing explosively and water and land resources are gradually being exhausted due to pollution. Smart agriculture is regarded as having an essential role in addressing the above challenges. Smart agriculture can significantly improve the agro-ecological environment and the yield and quality of agricultural products, and it can reduce the usage of pesticides and chemical fertilizers, thus alleviating the pollution of farmland and improving the sustainability of agricultural activities. The key to smart agriculture is in utilizing information and communication technologies to make agricultural cultivation and production automatic and intelligent. Specifically, wireless communications play an active role in the development of agriculture, and every generation of wireless communication technology drives agriculture to a more intelligent stage. In this article, we first review the wireless technologies which have mature applications in agriculture. Moreover, it is of importance to exploit the up-to-date communication technologies to further promote agricultural development. Therefore, we have surveyed the key technologies in sixth-generation mobile communication systems, as well as their existing and potential applications in smart agriculture.
Method of Bidirectional Green Wave Coordinated Control for Arterials under Asymmetric Release Mode
Authors: Ji Lina , Cheng Wei
Published: September 2022

The existing coordinated control methods of green wave are complicated, difficult to operate and mainly applicable to intersection groups with symmetrical arriving upstream flows. Based on engineering practice, a new method of bidirectional progression green wave coordination control was presented by designing particular overlapping phases on the basis of NEMA dual-ring phasing configuration. Applying the characteristics of asymmetric release mode and the requirements of green wave coordinated control, the overall optimization designs of phase sequence combination and offset were carried out, and the influences of cruising speed and residual queues at red light on offset were considered, and then the classical bidirectional green wave graphic method was optimized. Based on the investigation data of the intersections group of Ziwu Road in Qujing City, bidirectional green wave designs were conducted under both symmetric and asymmetric release mode. The results show that the latter approach not only improved the bandwidth of bidirectional green wave band effectively, but also reduced the average delay and the average number of stops on the main road.
Industrial Pilot for Assessment of Polymeric and Ceramic Membrane Efficiency in Treatment of Liquid Digestate from Biogas Power Plant
Authors: Yfantis Alexandros , Yfantis Nikos , Angelakopoulou Triantafyllia , Giannakakis George , Michelet Fabien , Dokianakis Spyros , Vasilaki Evangelia , Katsarakis Nikos
Published: September 2022

Due to the depletion of available water resources and the consistently rising environmental pollution levels, the exploitation of the digestate generated as an unfavorable by-product of the industrial wastewater treatment plants, could not only offer a readily available source of recycled water, but also an efficient agricultural fertilizer. However, the first step for the utilization of the digestate is the removal of any potentially harmful contaminants, and ultrafiltration membranes can provide successful remediation routes in this direction. This work investigates the industrial pilot-scale purification and reusability of the liquid digestate derived from the anaerobic treatment of waste mixtures of high organic content, using ultrafiltration membrane technology. Two different types of ultrafiltration membranes, polymeric and ceramic, were evaluated regarding their efficiency and long-term performance, parameters that heavily affect the overall costs of the operational unit. Our results indicate that the ceramic membranes exhibited a superior performance compared to its polymeric analogues, such as a higher flux, as well as significantly increased lifetime, signifying promising cost-effective and long-term applicability on an industrial level. In addition, the analytical physicochemical characterization of the ultrafiltration reject indicated its high nutrient value, suggesting its highly promising exploitation as an added value fertilizer, further enhancing the sustainability of the proposed approach.
Spark Ignition Engine Modeling Using Optimized Artificial Neural Network
Authors: Tosso Hilkija Gaïus , Jardim Saulo Anderson Bibiano , Bloise Rafael , Santos Max Mauro Dias
Published: September 2022

The spark ignition engine is a complex multi-domain system that contains many variables to be controlled and managed with the aim of attending to performance requirements. The traditional method and workflow of the engine calibration comprise measure and calibration through the design of an experimental process that demands high time and costs on bench testing. For the growing use of virtualization through artificial neural networks for physical systems at the component and system level, we came up with a likely efficiency adoption of the same approach for the case of engine calibration that could bring much better cost reduction and efficiency. Therefore, we developed a workflow integrated into the development cycle that allows us to model an engine black-box model based on an auto-generated feedfoward Artificial Neural Network without needing the human expertise required by a hand-crafted process. The model’s structure and parameters are determined and optimized by a genetic algorithm. The proposed method was used to create an ANN model for injection parameters calibration purposes. The experimental results indicated that the method could reduce the time and costs of bench testing.
Real-Time Simulation of a Small Modular Reactor in-the-Loop within Nuclear-Renewable Hybrid Energy Systems
Authors: Gabbar Hossam A. , Esteves Otavio Lopes Alves
Published: September 2022

Advanced small modular reactors (SMRs) have recently been developed in many designs; therefore, nuclear energy stands out as a promising alternative to sustainability and reliability in replacing fossil fuel energies in microgrids. SMRs have been shown as the best option due to the fact of their lower initial capital, greater scalability, and siting flexibility compared to large nuclear plants. Nowadays, there are several simulators able to reproduce all the safety and control mechanics of different nuclear reactors; however, there exists a lack of emulators able to put these functionalities into a real scenario to ensure the feasibility of the use of nuclear energy within energy systems, especially in nonconventional systems. This paper aims to mimic the central control system of SMRs by modeling the nuclear processes aiming to contribute to real-time simulations using SMRs integrated with renewable energy in microgrids that could be applied for different scenarios, such as cogeneration systems or fast-charging stations for electric vehicles, by considering the impact on dispatch and reliability. The simulation process of the proposed model was validated experimentally using the hardware-in-the-loop technique, which consisted of the modeling being integrated into the hardware and tested using real-time simulators. The proposed system, also denominated as SMR-in-the-Loop, was designed and adapted to be easily integrated with existing microgrid systems to represent the behavior of an SMR in nuclear-renewable hybrid energy systems, avoiding high investments and complexity in testing and implementing actual nuclear reactors.
Microgrid Energy Management during High-Stress Operation
Authors: Price Thomas , Parker Gordon , Vaucher Gail , Jane Robert , Berman Morris
Published: September 2022

We consider the energy management of an isolated microgrid powered by photovoltaics (PV) and fuel-based generation with limited energy storage. The grid may need to shed load or energy when operating in stressed conditions, such as when nighttime electrical loads occur or if there is little energy storage capacity. An energy management system (EMS) can prevent load and energy shedding during stress conditions while minimizing fuel consumption. This is important when the loads are high priority and fuel is in short supply, such as in disaster relief and military applications. One example is a low-power, provisional microgrid deployed temporarily to service communication loads immediately after an earthquake. Due to changing circumstances, the power grid may be required to service additional loads for which its storage and generation were not originally designed. An EMS that uses forecasted load and generation has the potential to extend the operation, enhancing the relief objectives. Our focus was to explore how using forecasted loads and PV generation impacts energy management strategy performance. A microgrid EMS was developed exploiting PV and load forecasts to meet electrical loads, harvest all available PV, manage storage and minimize fuel consumption. It used a Model Predictive Control (MPC) approach with the instantaneous grid storage state as feedback to compensate for forecasting errors. Four scenarios were simulated, spanning a stressed and unstressed grid operation. The MPC approach was compared to a rule-based EMS that did not use load and PV forecasting. Both algorithms updated the generator’s power setpoint every 15 min, where the grid’s storage was used as a slack asset. While both methods had similar performance under unstressed conditions, the MPC EMS showed gains in storage management and load shedding when the microgrid was stressed. When the initial storage was low, the rule-based EMS could not meet the load requirements and shed 16% of the day’s electrical load. In contrast, the forecast-based EMS managed the load requirements for this scenario without shedding load or energy. The EMS sensitivity to forecast error was also examined by introducing load and PV generation uncertainty. The MPC strategy successfully corrected the errors through storage management. Since weather affects both PV energy generation and many types of electrical loads, this work suggests that weather forecasting advances can improve remote microgrid performance in terms of fuel consumption, load satisfaction, and energy storage requirements.
Economic and Environmental Analysis of Solar Thermal and Seasonal Thermal Energy Storage Based on a Renewable Energy Conversion System for Greenhouses
Authors: Kim Deuk-Won , Kim Min-Hwi , Lee Dong-Won
Published: September 2022

This study investigates the economic benefits of solar thermal and seasonal thermal energy storage based on a renewable energy conversion system for greenhouses. The proposed system consists of solar collectors, seasonal thermal energy storage, hybrid-source heat pumps, and ground-source heat pumps. The heat generated from the proposed system was stored in two types of seasonal thermal energy storage and supplied to the greenhouse using Purme Yeoju Farm in South Korea for experimental analysis. Based on the experimental data gathered over a heating system, the economic benefits of operating cost savings and carbon trading with the greenhouse gas emission reduction of the proposed system were investigated by comparing to a conventional heating season using oil and electric boilers. From October 2021 to March 2022, approximately 38.4% of the total 482 MWh of heat was supplied either directly or indirectly through the solar system. In addition, the coefficient of the performance of the entire proposed system was calculated to be 2.28. Both the operating cost savings and greenhouse gas emission reductions of the proposed system showed over 73% and 82% compared with those of conventional systems.
Study on the Combustion Characteristics of Mountain Forest Vegetation
Authors: Jia Jiacheng , Huang Rui , Wang Yi
Published: September 2022

Leaves from four common tree species in Chinese mountain forests, namely, Symplocos setchuensis Brand, Tarenna mollissima, Loropetalum chinense, and Castanopsis sclerophylla were studied to assess their ignitability. The microstructure of each sample was determined to investigate its effect on combustion performance. Differential thermogravimetric analysis–differential scanning calorimetry (DTA–DSC) was performed to characterize thermal decomposition processes and relate them to flammability and fire resistance. In addition, pyrolysis reaction kinetic models were built, and fitting results were obtained in order to estimate the ignitability of the different abovementioned tree types. In this paper, the activation energy of the lignin decomposition stage was used to determine the fire hazard and ignitability. Regression analysis and correlation tests of microstructural parameters were performed. The results indicated that Castanopsis sclerophylla possessed the greatest fire hazard, followed by Loropetalum chinense, Tarenna mollissima, and Symplocos setchuensis Brand. The results of this study can provide a practical basis for the selection of fire-resistant tree species and forest belts.
RETRACTED: Evaluation of Soil Pollution by Potential Toxic Elements in Cultivated Land in the Poyang Lake Region Based on an Improved Matter–Element Extension Model
Authors: Luo Zhijun , Zhao Jie , Qi Song , Feng Xiaojing
Published: September 2022

This study examined the pollution status of potential toxic elements (PTEs) in cultivated soils throughout the Poyang Lake region, Jiangxi Province, China. A total of 251 topsoil samples were collected from the 0–20 cm depth to determine the concentrations of seven PTEs (Hg, As, Pb, Cd, Cu, Cr, and Zn). Based on the concentrations of PTEs, we constructed an improved matter–element extension model to evaluate the level of soil pollution by PTEs. We also applied Hakanson’s toxic response factor to correct the weights of PTEs determined by a conventional weighting method. The mean concentrations of all seven PTEs in the soil samples exceeded their local background values in Jiangxi Province. The over-standard rates of different PTEs were ranked in the order of Cr > Cu > Cd > Hg > Zn > Pb > As (36.2–87.9%). These potentially harmful pollutants mainly came from the surrounding industrial and agricultural areas, and could enter cultivated soils through different pathways. Samples from Duchang County, Hukou County, and Gongqingcheng City were in a clean state, whereas samples from other areas was in a still clean state or at the warning limit. The evaluation results were consistent with those obtained using several conventional methods. The improved matter–element extension model can therefore be applied for the evaluation of soil pollution by PTEs and yield reliable results in cultivated land.
Jellyfish as Food: A Narrative Review
Authors: Raposo António , Alasqah Ibrahim , Alfheeaid Hani , Alsharari Zayed , Alturki Hmidan , Raheem Dele
Published: September 2022

Studies toward a sustainable future conducted by international organizations uniformly agree about having to change some of our present consumer behaviors. Regarding food, suggestions include eating locally farmed, less industrialized and renewable food to promote health and circularity, and limiting waste. Jellyfish are frequently sorted and discarded after being caught with fish in fishing nets and gear. In contrast, we propose utilizing this by-catch as food. This review discusses the economic value and sustainability of jellyfish, the technologies used to prepare them for human consumption, their nutritional profile and health impacts and, finally, consumer acceptability and sensory evaluation of jellyfish food products. This discussion is critical for promoting jellyfish as an important aquatic resource to support blue and circular economies.
Experiences of Nurses in Nursing Homes during the COVID-19 Pandemic in Germany: A Qualitative Study
Authors: Diehl Elisabeth , Hiss David , Wege Aline , Hirschmüller Anna , Nienhaus Albert , Letzel Stephan
Published: September 2022

(1) Background: The aim of this qualitative study was to examine pandemic-related changes in nursing work in nursing homes, the resulting work-related stresses and external as well as internal alleviating measures. (2) Methods: We conducted 10 interviews from March to June 2021 with nurses from eight facilities. Data were analysed according to qualitative content analysis. (3) Results: Nurses faced increased workloads due to regulations and guidelines paired with staffing shortages. Work became more difficult due to personal protective equipment (PPE), conflict with residents’ relatives and, in the case of outbreaks, excess death and suffering. Nurse-to-resident care work became more emotionally demanding, with residents more distressed due to the lockdown, while families and social workers were not allowed into the facility. Residents with dementia posed an additional challenge, as they did not remember hygiene and distancing rules. Internal and external measures were not sufficient to alleviate the situation. However, some measures, such as training programmes or existing palliative care concepts, were considered helpful. (4) Conclusions: Facing other possible upcoming pandemics, ways to improve facility administration to prepare for future pandemics are highly needed, such as regular training programmes to prepare for possible lockdown scenarios, PPE use or potential hygiene measures.
Altruism in Paramedicine: A Scoping Review
Authors: Parker Leigh , Prior Sarah J. , Van Dam Pieter J. , Edwards Dale G.
Published: September 2022

While altruism has been studied in healthcare professions such as nursing and medicine, the exploration of the characteristics of altruism, as related to paramedicine and emergency care in Australia, is limited. This scoping review explores altruism in paramedicine from the perspective of the paramedic as practitioner, learner, and educator as seen through the lens of the paramedic and the patient. Also discussed is the positive impact of altruism on the patient experience of care. A scoping review was used to assess the availability of data related to altruism in paramedicine. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews was used to guide the process. Search categories were orientated around the subject (altruism) and discipline (paramedicine). A total of 27 articles are included in this scoping review. Initial searching identified 742 articles; after duplicate removal, 396 articles were screened with 346 excluded. Fifty articles were full-text reviewed and 23 excluded. The final 27 were extracted following full-text screening. None of the articles are specific to altruism in paramedicine. The data related to the practice of altruism in paramedicine are extremely limited. The preponderance of data arise from Europe and North America which, due to crewing and service differences, may impact the practice of altruism in different regions. Recent changes to the scope of paramedic practice, workload, education, and case acuity may influence behaviour regarding altruism, compassion, caring, and associated caring behaviours. The practice and education of paramedics including altruism, compassion, caring and caring behaviours in the Australasian setting warrants further research.
Epilepsy Seizures Prediction Based on Nonlinear Features of EEG Signal and Gradient Boosting Decision Tree
Authors: Xu Xin , Lin Maokun , Xu Tingting
Published: September 2022

Epilepsy is a common neurological disorder with sudden and recurrent seizures. Early prediction of seizures and effective intervention can significantly reduce the harm suffered by patients. In this paper, a method based on nonlinear features of EEG signal and gradient boosting decision tree (GBDT) is proposed for early prediction of epilepsy seizures. First, the EEG signals were divided into two categories: those that had seizures onset over a period of time (represented by InT) and those that did not. Second, the noise in the EEG was removed using complementary ensemble empirical mode decomposition (CEEMD) and wavelet threshold denoising. Third, the nonlinear features of the two categories of EEG were extracted, including approximate entropy, sample entropy, permutation entropy, spectral entropy and wavelet entropy. Fourth, a GBDT classifier with random forest as the initial result was designed to distinguish the two categories of EEG. Fifth, a two-step “k of n” method was used to reduce the number of false alarms. The proposed method was evaluated on 13 patients’ EEG data from the CHB-MIT Scalp EEG Database. Based on ten-fold cross validation, the average accuracy was 91.76% when the InT was taken at 30 min, and 38 out of 39 seizures were successfully predicted. When the InT was taken for 40 min, the average accuracy was 92.50% and all 42 seizures selected were successfully predicted. The results indicate the effectiveness of the proposed method for predicting epilepsy seizures.
The Association between Osteoporosis and Peripheral Artery Disease: A Population-Based Longitudinal Follow-Up Study in Taiwan
Authors: Syu De-Kai , Hsu Shu-Hua , Yeh Ping-Chun , Lee Tsung-Lin , Kuo Yu-Feng , Huang Yen-Chun , Jiang Ching-Chuan , Chen Mingchih
Published: September 2022

Background: There are several possible links that have been used to claim that osteoporosis and peripheral artery disease (PAD) are associated; however, the solid evidence is not sufficient. This study aimed to use the Taiwan National Health Insurance Research Database (NHIRD) to determine if osteoporosis is associated with peripheral artery disease (PAD). Method: NHIRD records from 23 million patients were collected to recruit two matched cohort groups: 64,562 patients with and 64,562 patients without osteoporosis. To compare the crude hazard ratio (HR) and the incidence rate ratio between the two cohort groups for PAD, the Cox model was used. Result: With an adjusted HR of 1.18 (95% CI, 1.08–1.29), the osteoporosis cohort group had a significantly greater risk for PAD than the group without osteoporosis. The cumulative incidence of PAD in the cohort group was also statistically higher than it was in the group without osteoporosis (1.71% and 1.39%; p ≤ 0.0001, log-rank) over the 10-year follow-up period. In addition, the osteoporotic patients with ischemic stroke, chronic obstructive pulmonary disease (COPD), and congestive heart failure (CHF) had a significantly increased risk of PAD based on subgroup analysis. Conclusions: There was a positive association between osteoporosis and the development of PAD, as patients with osteoporosis had an increased incidence of PAD over time.
Difference in Mortality Rates by Occupation in Japanese Male Workers Aged 25 to 64 Years from 1980 to 2015
Authors: Dhungel Bibha , Murakami Tomoe , Wada Koji , Ikeda Shunya , Gilmour Stuart
Published: September 2022

This study examines the trends in mortality among Japanese working men, across various occupational categories, from 1980 to 2015. A Poisson model of trend, occupational category, and step variable was analysed for eight occupational categories separately, by cause, to explore the trends in mortality. This study found a sharp increase in mortality in the late 1990s, especially among professionals and managers. The overall trends in cancer, ischemic heart disease (IHD), cerebrovascular disease (CVD), and suicide mortality decreased across almost all occupational categories from 1980 to 2015, although there was an increasing trend in cancer of 0.5% among managers. Clerical workers had the greatest relative decrease in mortality rates from cancer (−82.9%), IHD (−81.7%), and CVD (−89.1%). Japan continues to make gains in lowering mortality and extending life expectancy, but its workplace culture must improve to ensure that those working at the heart of the Japanese corporate world can also benefit from Japan’s progress in health. Mortality rates in working-aged Japanese men have been declining. However, similar declines are not evident among managers, for whom the mortality rate is remaining stable or slightly increasing. There is a need to address the needs of managers and improve workplace environments for these workers.
Colitis Induces Sex-Specific Intestinal Transcriptomic Responses in Mice
Authors: Hases Linnea , Birgersson Madeleine , Indukuri Rajitha , Archer Amena , Williams Cecilia
Published: September 2022

There are significant sex differences in colorectal cancer (CRC), including in incidence, onset, and molecular characteristics. Further, while inflammatory bowel disease (IBD) is a risk factor for CRC in both sexes, men with IBD have a 60% higher risk of developing CRC compared to women. In this study, we investigated sex differences during colitis-associated CRC (CAC) using a chemically induced CAC mouse model. The mice were treated with azoxymethane (AOM) and dextran sodium sulfate (DSS) and followed for 9 and 15 weeks. We performed RNA-sequencing of colon samples from males (n = 15) and females (n = 15) to study different stages of inflammation and identify corresponding transcriptomic sex differences in non-tumor colon tissue. We found a significant transcriptome response to AOM/DSS treatment in both sexes, including in pathways related to inflammation and cell proliferation. Notably, we found a stronger response in males and that male-specific differentially expressed genes were involved in NFκB signaling and circadian rhythm. Further, an overrepresented proportion of male-specific gene regulations were predicted to be targets of Stat3, whereas for females, targets of the glucocorticoid receptor (Gr/Nr3c1) were overrepresented. At 15 weeks, the most apparent sex difference involved genes with functions in T cell proliferation, followed by the regulation of demethylases. The majority of sex differences were thus related to inflammation and the immune system. Our novel data, profiling the transcriptomic response to chemically induced colitis and CAC, indicate clear sex differences in CRC initiation and progression.
Hypoxia-Inducible Factors and Diabetic Kidney Disease—How Deep Can We Go?
Authors: Stanigut Alina Mihaela , Pana Camelia , Enciu Manuela , Deacu Mariana , Cimpineanu Bogdan , Tuta Liliana Ana
Published: September 2022

Diabetes is one of the leading causes of chronic kidney disease (CKD), and multiple underlying mechanisms involved in pathogenesis of diabetic nephropathy (DN) have been described. Although various treatments and diagnosis applications are available, DN remains a clinical and economic burden, considering that about 40% of type 2 diabetes patients will develop nephropathy. In the past years, some research found that hypoxia response and hypoxia-inducible factors (HIFs) play critical roles in the pathogenesis of DN. Hypoxia-inducible factors (HIFs) HIF-1, HIF-2, and HIF-3 are the main mediators of metabolic responses to the state of hypoxia, which seems to be the one of the earliest events in the occurrence and progression of diabetic kidney disease (DKD). The abnormal activity of HIFs seems to be of crucial importance in the pathogenesis of diseases, including nephropathies. Studies using transcriptome analysis confirmed by metabolome analysis revealed that HIF stabilizers (HIF-prolyl hydroxylase inhibitors) are novel therapeutic agents used to treat anemia in CKD patients that not only increase endogenous erythropoietin production, but also could act by counteracting the metabolic alterations in incipient diabetic kidney disease and relieve oxidative stress in the renal tissue. In this review, we present the newest data regarding hypoxia response and HIF involvement in the pathogenesis of diabetic nephropathy and new therapeutic insights, starting from improving kidney oxygen homeostasis.
TIAM2 Contributes to Osimertinib Resistance, Cell Motility, and Tumor-Associated Macrophage M2-like Polarization in Lung Adenocarcinoma
Authors: Liang Lu , He Hua , Jiang Shiyao , Liu Yueying , Huang Jingjing , Sun Xiaoyan , Li Yi , Jiang Yiqun , Cong Li
Published: September 2022

Background: Osimertinib-based therapy effectively improves the prognosis of lung adenocarcinoma (LUAD) patients with epidermal growth factor receptor mutations. However, patients will have cancer progression after approximately one year due to the occurrence of drug resistance. Extensive evidence has revealed that lipid metabolism and tumor-associated macrophage (TAM) are associated with drug resistance, which deserves further exploration. Methods: An osimertinib resistance index (ORi) was built to investigate the link between lipid metabolism and osimertinib resistance. The ORi was constructed and validated using TCGA and GEO data, and the relationship between ORi and immune infiltration was discussed. Weighted gene co-expression network analysis based on the M2/M1 macrophage ratio determined the hub gene TIAM2 and the biological function of TIAM2 in LUAD was verified in vitro. Results: ORi based on nine lipid metabolism-related genes was successfully constructed, which could accurately reflect the resistance of LUAD patients to osimertinib, predict the prognosis, and correlate with M2-like TAM. Additionally, TIAM2 was found to increase osimertinib tolerance, enhance cell motility, and promote M2-like TAM polarization in LUAD. Conclusions: The lipid metabolism gene is strongly connected with osimertinib resistance. TIAM2 contributes to osimertinib resistance, enhances cell motility, and induces M2-like TAM polarization in LUAD.
Human Monocyte-Derived Dendritic Cells Are the Pharmacological Target of the Immunosuppressant Flavonoid Silibinin
Authors: Pagano Maria Teresa , Fecchi Katia , Pierdominici Marina , Ortona Elena , Peruzzu Daniela
Published: September 2022

Silibinin, a natural polyphenolic flavonoid, is known to possess anti-inflammatory, anticancer, antioxidant, and immunomodulatory properties. However, the effects of Silibinin on the maturation and immunostimulatory functions of human dendritic cells (DC) remain to be elucidated. In this study, we have attempted to ascertain whether Silibinin influences the maturation, cytokine production, and antigen-presenting capacity of human monocyte-derived DC. We show that Silibinin significantly suppresses the upregulation of costimulatory and MHC molecules in LPS-stimulated mature DC and inhibits lipopolysaccharide (LPS)-induced interleukin (IL)-12, IL-23, and TNF-α production. Furthermore, Silibinin impairs the proliferation response of the allogenic memory CD4 T lymphocytes elicited by LPS-matured DC and their Th1/Th17 profile. These findings demonstrate that Silibinin displays immunosuppressive activity by inhibiting the maturation and activation of human DC and support its potential application of adjuvant therapy in the treatment of autoimmune diseases.
Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides
Authors: Liao Jingjing , Liu Tingyao , Xie Lei , Mo Changming , Huang Xiyang , Cui Shengrong , Jia Xunli , Lan Fusheng , Luo Zuliang , Ma Xiaojun
Published: September 2022

Mogrosides are a group of health-promoting natural products that extracted from Siraitia grosvenorii fruit (Luo-han-guo or monk fruit), which exhibited a promising practical application in natural sweeteners and pharmaceutical development. However, the production of mogrosides is inadequate to meet the need worldwide, and uneconomical synthetic chemistry methods are not generally recommended for structural complexity. To address this issue, an in-fusion based gene stacking strategy (IGS) for multigene stacking has been developed to assemble 6 mogrosides synthase genes in pCAMBIA1300. Metabolic engineering of Nicotiana benthamiana and Arabidopsis thaliana to produce mogrosides from 2,3-oxidosqualene was carried out. Moreover, a validated HPLC-MS/MS method was used for the quantitative analysis of mogrosides in transgenic plants. Herein, engineered Arabidopsis thaliana produced siamenoside I ranging from 29.65 to 1036.96 ng/g FW, and the content of mogroside III at 202.75 ng/g FW, respectively. The production of mogroside III was from 148.30 to 252.73 ng/g FW, and mogroside II-E with concentration between 339.27 and 5663.55 ng/g FW in the engineered tobacco, respectively. This study provides information potentially applicable to develop a powerful and green toolkit for the production of mogrosides.
Electrical Insulation and Radar-Wave Absorption Performances of Nanoferrite/Liquid-Silicone-Rubber Composites
Authors: Sun Wei-Feng , Sun Peng-Bo
Published: September 2022

Novel radar-wave absorption nanocomposites are developed by filling the nanoscaled ferrites of strontium ferroxide (SrFe12O19) and carbonyl iron (CIP) individually into the highly flexible liquid silicone rubber (LSR) considered as dielectric matrix. Nanofiller dispersivities in SrFe12O19/LSR and CIP/LSR nanocomposites are characterized by scanning electronic microscopy, and the mechanical properties, electric conductivity, and DC dielectric-breakdown strength are tested to evaluate electrical insulation performances. Radar-wave absorption performances of SrFe12O19/LSR and CIP/LSR nanocomposites are investigated by measuring electromagnetic response characteristics and radar-wave reflectivity, indicating the high radar-wave absorption is dominantly derived from magnetic losses. Compared with pure LSR, the SrFe12O19/LSR and CIP/LSR nanocomposites represent acceptable reductions in mechanical tensile and dielectric-breakdown strengths, while rendering a substantial nonlinearity of electric conductivity under high electric fields. SrFe12O19/LSR nanocomposites provide high radar-wave absorption in the frequency band of 11~18 GHz, achieving a minimum reflection loss of −33 dB at 11 GHz with an effective absorption bandwidth of 10 GHz. In comparison, CIP/LSR nanocomposites realize a minimum reflection loss of −22 dB at 7 GHz and a remarkably larger effective absorption bandwidth of 3.9 GHz in the lower frequency range of 2~8 GHz. Radar-wave transmissions through SrFe12O19/LSR and CIP/LSR nanocomposites in single- and double-layered structures are analyzed with CST electromagnetic-field simulation software to calculate radar reflectivity for various absorbing-layer thicknesses. Dual-layer absorbing structures are modeled by specifying SrFe12O19/LSR and CIP/LSR nanocomposites, respectively, as match and loss layers, which are predicted to acquire a significant improvement in radar-wave absorption when the thicknesses of match and loss layers approach 1.75 mm and 0.25 mm, respectively.
Loss of Serpina1 in Mice Leads to Altered Gene Expression in Inflammatory and Metabolic Pathways
Authors: Meghadri Sri Harsha , Martinez-Delgado Beatriz , Ostermann Lena , Gomez-Mariano Gema , Perez-Luz Sara , Tumpara Srinu , Wrenger Sabine , DeLuca David S. , Maus Ulrich A. , Welte Tobias , Janciauskiene Sabina
Published: September 2022

The SERPINA1 gene encodes alpha1-antitrypsin (AAT), an acute phase glycoprotein and serine protease inhibitor that is mainly (80–90%) produced in the liver. Point mutations in the SERPINA1 gene can lead to the misfolding, intracellular accumulation, and deficiency of circulating AAT protein, increasing the risk of developing chronic liver diseases or chronic obstructive pulmonary disease. Currently, siRNA technology can knock down the SERPINA1 gene and limit defective AAT production. How this latter affects other liver genes is unknown. Livers were taken from age- and sex-matched C57BL/6 wild-type (WT) and Serpina1 knockout mice (KO) aged from 8 to 14 weeks, all lacking the five serpin A1a-e paralogues. Total RNA was isolated and RNA sequencing, and transcriptome analysis was performed. The knockout of the Serpina1 gene in mice changed inflammatory, lipid metabolism, and cholesterol metabolism-related gene expression in the liver. Independent single-cell sequencing data of WT mice verified the involvement of Serpina1 in cholesterol metabolism. Our results from mice livers suggested that designing therapeutic strategies for the knockout of the SERPINA1 gene in humans must account for potential perturbations of key metabolic pathways and consequent mitigation of side effects.
Progress of Nanomaterials-Based Photothermal Therapy for Oral Squamous Cell Carcinoma
Authors: Niu Qin , Sun Qiannan , Bai Rushui , Zhang Yunfan , Zhuang Zimeng , Zhang Xin , Xin Tianyi , Chen Si , Han Bing
Published: September 2022

Oral squamous cell carcinoma (OSCC) is one of the top 15 most prevalent cancers worldwide. However, the current treatment models for OSCC (e.g., surgery, chemotherapy, radiotherapy, and combination therapy) present several limitations: damage to adjacent healthy tissue, possible recurrence, low efficiency, and severe side effects. In this context, nanomaterial-based photothermal therapy (PTT) has attracted extensive research attention. This paper reviews the latest progress in the application of biological nanomaterials for PTT in OSCC. We divide photothermal nanomaterials into four categories (noble metal nanomaterials, carbon-based nanomaterials, metal compounds, and organic nanomaterials) and introduce each category in detail. We also mention in detail the drug delivery systems for PTT of OSCC and briefly summarize the applications of hydrogels, liposomes, and micelles. Finally, we note the challenges faced by the clinical application of PTT nanomaterials and the possibility of further improvement, providing direction for the future research of PTT in OSCC treatment.
A Review of the Emerging Poultry Visceral Gout Disease Linked to Avian Astrovirus Infection
Authors: Li Linlin , Sun Minhua , Zhang Yun , Liao Ming
Published: September 2022

Avian astroviruses, including chicken astrovirus (CAstV), avian nephritisvirus (ANV), and goose astrovirus (GoAstV), are ubiquitous enteric RNA viruses associated with enteric disorders in avian species. Recent research has found that infection of these astroviruses usually cause visceral gout in chicken, duckling and gosling. However, the underlying mechanism remains unknown. In the current article, we review recent discoveries of genetic diversity and variation of these astroviruses, as well as pathogenesis after astrovirus infection. In addition, we discuss the relation between avian astrovirus infection and visceral gout in poultry. Our aim is to review recent discoveries about the prevention and control of the consequential visceral gout diseases in poultry, along with the attempt to reveal the possible producing process of visceral gout diseases in poultry.