bluuebunny/crossref_metadata_2025 huggingface dataset:
Cryo-Electron Microscopy of Arabidopsis thaliana Phytochrome A in Its Pr State Reveals Head-to-Head Homodimeric Architecture
Authors: Wahlgren Weixiao Yuan , Golonka David , Westenhoff Sebastian , Möglich Andreas
Published: April 2021

Phytochrome photoreceptors regulate vital adaptations of plant development, growth, and physiology depending on the ratio of red and far-red light. The light-triggered Z/E isomerization of a covalently bound bilin chromophore underlies phytochrome photoconversion between the red-absorbing Pr and far-red-absorbing Pfr states. Compared to bacterial phytochromes, the molecular mechanisms of signal propagation to the C-terminal module and its regulation are little understood in plant phytochromes, not least owing to a dearth of structural information. To address this deficit, we studied the Arabidopsis thaliana phytochrome A (AtphyA) at full length by cryo-electron microscopy (cryo-EM). Following heterologous expression in Escherichia coli, we optimized the solvent conditions to overcome protein aggregation and thus obtained photochemically active, near-homogenous AtphyA. We prepared grids for cryo-EM analysis of AtphyA in its Pr state and conducted single-particle analysis. The resulting two-dimensional class averages and the three-dimensional electron density map at 17 Å showed a homodimeric head-to-head assembly of AtphyA. Docking of domain structures into the electron density revealed a separation of the AtphyA homodimer at the junction of its photosensor and effector modules, as reflected in a large void in the middle of map. The overall architecture of AtphyA resembled that of bacterial phytochromes, thus hinting at commonalities in signal transduction and mechanism between these receptors. Our work paves the way toward future studies of the structure, light response, and interactions of full-length phytochromes by cryo-EM.
Developing Resilience During the COVID-19 Pandemic: Yoga and Mindfulness for the Well-Being of Student Musicians in Spain
Authors: Bartos L. Javier , Funes María J. , Ouellet Marc , Posadas M. Pilar , Krägeloh Chris
Published: April 2021

Here, we report on a quasi-experimental study to explore the applicability and perceived benefits of the CRAFT program, which is based on mindfulness, yoga, positive psychology, and emotional intelligence, to improve higher education student musicians’ health and well-being during the lockdown. A subset of student musicians at a Higher Conservatory of Music in Spain followed the CRAFT program during the academic year 2019/2020, 1 h per week as part of their curriculum. Students enrolled in CRAFT-based elective subjects formed the CRAFT program group (n = 40), while other students represented the control group (n = 53). The onset of the national lockdown elicited by the COVID-19 pandemic occurred halfway through the program, which was subsequently delivered in an online format. We administered an online survey to explore the effect that the exposure to the CRAFT program had in terms of how participants dealt with various health and well-being concerns arising from the COVID-19 lockdown. There was a significantly higher proportion of proactive participants in the CRAFT program group, 92%, than in the control group, 58%, in terms of implementing practices to improve their health and well-being during the lockdown. Additionally, significantly more participants acknowledged perceived benefits from their practices in the CRAFT program group, 78%, than in the control group, 52%. Among proactive participants, yoga/meditation was the most implemented in the CRAFT program group, followed by exercise, and other yoga/meditation practices, whereas in the control group, exercise and Alexander technique-based practices were the most applied. In the CRAFT program group, the highest rate of perceived benefits was from yoga/meditation CRAFT-based practices, 51%, followed by exercise, 32%, and other yoga/meditation practices, 27%, whereas in the control group, benefits were reported by 29% of exercising participants and 16% for those having practiced the Alexander technique. A similar pattern was observed when excluding participants with previous yoga/meditation experience. This study revealed how participants can independently apply learned skills from the CRAFT program in response to a naturally occurring life event of unprecedented global impact, suggesting that previous exposure to mindfulness and yoga is likely to have a beneficial effect on how young adults react towards exceptionally stressful conditions.
Effectiveness of a Community-Based Muscle Strengthening Exercise Program to Increase Muscle Strength Among Pre-frail Older Persons in Malaysia: A Pilot Study
Authors: Raja Adnan Raja Nurzatul Efah , Mat Din Hazwan , Ashari Asmidawati , Minhat Halimatus Sakdiah
Published: April 2021

Deterioration in muscle mass and muscle strength is common among the frail older persons, cause functional dependence and decrease in the quality of life. Therefore, the identification of cost-effective interventions to prevent or ameliorate frailty is crucially needed. The aim of this study is to determine the effectiveness of a Community-based Muscle Strengthening Exercise (COME) program to increase muscle strength among pre-frail older persons. This study was a quasi-experimental study. A total of 32 older persons aged 60 years and older with pre-frail symptoms were recruited from the community center. The COME was developed based on the Growing Stronger program and the Otago Home Exercise Program. COME was designed to strengthen all of the major muscle groups in the upper and lower extremities. The exercise program was conducted for 12 weeks and divided into 3 parts; (1) to strengthen the body slowly and gently, using only body weight, (2) to introduce dumbbells and ankle weights to increase strength and (3) to add various new ways to boost strength even more. Functional tests were measured before and after the intervention. The results revealed non-significant p-value for pre- and post-intervention comparison for all study outcomes. Observing the values of mean difference, the study intervention was shown to have slightly improved the time up and go (Mean difference = −0.25), and sit-to-stand duration (Mean difference = −0.41) as well as the handgrip strength (Mean difference = 0.68) among the participants. On the assessment of Cohen ES, all three improvements exhibited small effect sizes. Sit-to-stand duration was shown to have most benefited from the intervention with highest ES among the outcome variables (ES = 0.20). COME intervention program among pre-frail older persons showed favorable trend toward improvement of upper and lower extremities muscle strength. This study should be further tested in randomized control trial to confirm its effectiveness.
Improving Human Health in China Through Alternative Energy
Authors: Scott Melissa , Sander Robert , Nemet Gregory , Patz Jonathan
Published: April 2021

In this study, we estimate the health benefits of more stringent alternative energy goals and the costs of reducing coal-fired power plant pollution in China projected in 2030. One of our two overarching alternative energy goals was to estimate the health benefits of complete elimination of coal energy, supplemented by natural gas and renewables. The second was a policy scenario similar to the U.S. 2013 Climate Action Plan (CAP), which played a pivotal role leading up to the 2015 Paris Climate Agreement. We used the Greenhouse Gas and Air Pollution Interactions and Synergies (GAINS) model created by the International Institute for Applied Systems Analysis for our model simulations. We found that 17,137–24,220 premature deaths can be avoided if coal energy is completely replaced by alternative energy, and 8,693–9,281 premature deaths can be avoided if coal energy is replaced by alternatives in a CAP-like scenario. A CAP-like scenario using emission-controls in coal plants costs $11–18 per person. Reducing coal energy in China under a CAP-like scenario would free up $9.4 billion in the annual energy budget to spend on alternatives, whereas eliminating the cost of coal energy frees up $32 billion. This study's estimates show that more stringent alternative energy targets in China are worth the investment in terms of health.
Male Involvement in Reproductive and Maternal and New Child Health: An Evaluative Qualitative Study on Facilitators and Barriers From Rural Kenya
Authors: Lusambili Adelaide M. , Muriuki Peter , Wisofschi Stefania , Shumba Constance S. , Mantel Michaela , Obure Jerim , Nyaga Lucy , Mulama Kennedy , Ngugi Anthony , Orwa James , Luchters Stanley , Temmerman Marleen
Published: April 2021

Male involvement in reproductive, maternal, newborn and child health (RMNCH) is known to improve maternal and child health outcomes. However, there is sub-optimal adoption of male involvement strategies in several low- and middle-income countries such as Kenya. Aga Khan University implemented Access to Quality of Care through Extending and Strengthening Health Systems (AQCESS), a project funded by the Government of Canada and Aga Khan Foundation Canada (AKFC), between 2016 and 2020 in rural Kisii and Kilifi counties, Kenya. A central element in the interventions was increasing male engagement in RMNCH. Between January and March 2020, we conducted an endline qualitative study to examine the perspectives of different community stakeholders, who were aware of the AQCESS project, on the facilitators and barriers to male involvement in RMNCH. We found that targeted information sessions for men on RMNCH are a major facilitator to effective male engagement, particularly when delivered by male authority figures such as church leaders, male champions and teachers. Sub-optimal male engagement arises from tensions men face in directly contributing to the household economy and participating in RMNCH activities. Social-cultural factors such as the feminization of RMNCH and the associated stigma that non-conforming men experience also discourage male engagement.
Feasibility of Using Commercially Available Accelerometers to Monitor Upper Extremity Home Practice With Persons Post-stroke: A Secondary Data Analysis
Authors: de Castro Kate N. , Donoso Brown Elena V. , Miller Neilan Rachael , Wallace Sarah E.
Published: April 2021

Background: Adherence to home practice rehabilitation programs is important for efficacy; however, adherence is challenging for many individuals post-stroke. Accelerometers have emerged as a potential means to support home practice. This secondary data analysis explored the use of a commercially available accelerometer with custom software to collect and analyze data to corroborate self-reported practice collected during a home program.

Methods: The initial study was a single subject design trial that investigated the effect of preferred music listening on adherence to an upper extremity home practice program (Trial Number NCT02906956. ClinicalTrials.gov). The participants (n = 7) were post-stroke adults with aphasia and hemiparesis of the upper extremity. Participants completed home program exercises while wearing accelerometers and recorded practice times in a logbook. Data were collected, cleaned, processed, and analyzed to facilitate descriptive comparisons and clinical interpretations of accelerometer output data.

Results: Across all participants, an average of 47% of data were captured and usable for analysis. Five out of seven participants self-reported longer practice times compared to accelerometer duration output by a mean of 66.5 s. Individual exercise set mean total angular velocity and standard deviation of acceleration demonstrated potential for use across time to monitor change.

Conclusions: One challenge of integrating accelerometers into clinical practice is the amount of data loss and the steps for data processing. The comparisons of available accelerometer data to the self-reported logs, however, were generally representative. Future investigations should explore ways to increase data capture and accessibility of the data for feedback to the client and practitioner.
Global Magnetohydrodynamic Simulations: Performance Quantification of Magnetopause Distances and Convection Potential Predictions
Authors: Mukhopadhyay Agnit , Jia Xianzhe , Welling Daniel T. , Liemohn Michael W.
Published: April 2021

The performance of three global magnetohydrodynamic (MHD) models in estimating the Earth's magnetopause location and ionospheric cross polar cap potential (CPCP) have been presented. Using the Community Coordinated Modeling Center's Run-on-Request system and extensive database on results of various magnetospheric scenarios simulated for a variety of solar weather patterns, the aforementioned model predictions have been compared with magnetopause standoff distance estimations obtained from six empirical models, and with cross polar cap potential estimations obtained from the Assimilative Mapping of Ionospheric Electrodynamics (AMIE) Model and the Super Dual Auroral Radar Network (SuperDARN) observations. We have considered a range of events spanning different space weather activity to analyze the performance of these models. Using a fit performance metric analysis for each event, the models' reproducibility of magnetopause standoff distances and CPCP against empirically-predicted observations were quantified, and salient features that govern the performance characteristics of the modeled magnetospheric and ionospheric quantities were identified. Results indicate mixed outcomes for different models during different events, with almost all models underperforming during the extreme-most events. The quantification also indicates a tendency to underpredict magnetopause distances in the absence of an inner magnetospheric model, and an inclination toward over predicting CPCP values under general conditions.
Three-Dimensional Elastodynamic Analysis Employing Partially Discontinuous Boundary Elements
Authors: Li Yuan , Zhang Ni , Gong Yuejiao , Mao Wentao , Zhang Shiguang
Published: April 2021

Compared with continuous elements, discontinuous elements advance in processing the discontinuity of physical variables at corner points and discretized models with complex boundaries. However, the computational accuracy of discontinuous elements is sensitive to the positions of element nodes. To reduce the side effect of the node position on the results, this paper proposes employing partially discontinuous elements to compute the time-domain boundary integral equation of 3D elastodynamics. Using the partially discontinuous element, the nodes located at the corner points will be shrunk into the element, whereas the nodes at the non-corner points remain unchanged. As such, a discrete model that is continuous on surfaces and discontinuous between adjacent surfaces can be generated. First, we present a numerical integration scheme of the partially discontinuous element. For the singular integral, an improved element subdivision method is proposed to reduce the side effect of the time step on the integral accuracy. Then, the effectiveness of the proposed method is verified by two numerical examples. Meanwhile, we study the influence of the positions of the nodes on the stability and accuracy of the computation results by cases. Finally, the recommended value range of the inward shrink ratio of the element nodes is provided.
β-Band Analysis from Simulated Flight Experiments
Authors: Roza Válber César Cavalcanti , Postolache Octavian
Published: April 2021

Several safety-related improvements are applied every year to try to minimize the total number of civil aviation accidents. Fortunately, these improvements work well, reducing the number of accident occurrences. However, while the number of accidents due to mechanical failures has decreased, the number of accidents due to human errors seems to grow. On that basis, this work presents a contribution regarding the brain’s β-band activities for different levels of volunteers’ expertise on flight simulator, i.e., experienced, mid-level and beginner, in which they acted as pilots in command during several simulated flights. Spectrogram analysis and statistical measurements of each volunteer’s brain’s β-band were carried out. These were based on seven flight tasks: takeoff, climb, cruise flight, descent, approach, final approach and landing. The results of the proposed experiment showed that the takeoff, approach and landing corresponded to the highest brain activities, i.e., close to 37.06–67.33% more than the brain activity of the other flight tasks: when some accidents were about to occur, the intensities of the brain activity were similar to those of the final approach task. When the volunteers’ expertise and confidence on flight simulation were considered, it was shown that the highest brain magnitudes and oscillations observed of more experienced and confident volunteers were on average close to 68.44% less, compared to less experienced and less confident volunteers. Moreover, more experienced and confident volunteers in general presented different patterns of brain activities compared to volunteers with less expertise or less familiarity with fight simulations and/or electronic games.
Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate
Authors: Zheng Changbing , Song Bo , Guo Qiuping , Zheng Jie , Li Fengna , Duan Yehui , Peng Can
Published: April 2021

This study aimed to investigate the effects of dietary beta-hydroxy beta-methyl butyrate (HMB) supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow) were selected and fed a basal diet supplemented either with 0 (control), 0.13%, 0.64%, or 1.28% HMB for 60 days. Throughout the experiments, they had free access to clean drinking water and diets. Data of this study were analyzed by one-way ANOVA using the SAS 8.2 software package, followed by a Tukey’s studentized range test to explore treatment effects. The results showed that compared to the control, 0.13% HMB decreased the intramuscular fat (IMF) content and increased polyunsaturated fatty acids (PUFAs) in Longissimus thoracis muscle (LTM), and increased the n3 PUFAs in soleus muscles (SM, p < 0.05). Moreover, HMB supplementation led to alterations in the mRNA expression of genes related to lipid metabolism. Serum metabolome profiling showed that in both LTM and SM of Bama Xiang mini-pigs, N-Methyl-l-glutamate was positively correlated with SFA and nummularine A was negatively correlated with C18:3n3 PUFA (p < 0.05). Therefore, N-Methyl-l-glutamate and nummularine A might be potential biomarkers of the HMB-supplemented group. These results suggested that dietary HMB supplementation could decrease the IMF content and increase n3 PUFAs as well as regulate the related metabolites (N-Methyl-l-glutamate and nummularine A) in the serum of pigs.
Facilitators and Barriers to Assistance Dog Puppy Raisers’ Engagement in Recommended Raising Practices
Authors: Mai Dac , Howell Tiffani , Benton Pree , Lewis Virginia , Evans Lynette , Bennett Pauleen C.
Published: April 2021

Many assistance dog providers use volunteer raisers to manage each puppy’s learning and daily experiences, which partly determines the puppy’s behavioural development. Therefore, it is important that raisers engage in recommended practices. Three common recommendations from the literature include frequent socialisation and consistent training for the puppies, and effective training for the raisers. However, what facilitates or hinders raisers’ engagement in these practices remains unclear. To understand this, we interviewed eight raisers (three men and five women) every month during their year-long puppy raising program, and pseudo-randomly selected 16 from 48 interviews for data analysis. Thematic analyses revealed several facilitating and/or hindering factors corresponding to each of the three recommended practices. Frequent socialisation was influenced by the raisers’ availability, sharing of puppy raising responsibility with others, support from their workplace, and the puppy’s behaviours (e.g., soiling indoors, jumping). Consistent training was challenged by the presence of everyday distractors, accessibility to timely advice, perceived judgement from others, and the puppy’s undesirable behaviours. Effective learning was facilitated by having information available in raisers’ preferred learning modality, opportunities for peer-learning, and willingness to seek help. Future research should examine these factors quantitatively, which will enable more robust evaluation of programs aimed at supporting puppy raisers.
Evaluation of Aerosol Therapy during the Escalation of Care in a Model of Adult Cystic Fibrosis
Authors: Fernández Fernández Elena , Joyce Mary , O’Sullivan Andrew , MacLoughlin Ronan
Published: April 2021

Lung disease is the main cause of morbidity and mortality in cystic fibrosis (CF). CF patients inhale antibiotics regularly as treatment against persistent bacterial infections. The goal of this study was to investigate the effect of clinical intervention on aerosol therapy during the escalation of care using a bench model of adult CF. Droplet size analysis of selected antibiotics was completed in tandem with the delivered aerosol dose (% of total dose) assessments in simulations of various interventions providing oxygen supplementation or ventilatory support. Results highlight the variability of aerosolised dose delivery. In the homecare setting, the vibrating mesh nebuliser (VMN) delivered significantly more than the jet nebuliser (JN) (16.15 ± 0.86% versus 6.51 ± 2.15%). In the hospital setting, using VMN only, significant variability was seen across clinical interventions. In the emergency department, VMN plus mouthpiece (no supplemental oxygen) was seen to deliver (29.02 ± 1.41%) versus low flow nasal therapy (10 L per minute (LPM) oxygen) (1.81 ± 0.47%) and high flow nasal therapy (50 LPM oxygen) (3.36 ± 0.34%). In the ward/intensive care unit, non-invasive ventilation recorded 19.02 ± 0.28%, versus 22.64 ± 1.88% of the dose delivered during invasive mechanical ventilation. These results will have application in the design of intervention-appropriate aerosol therapy strategies and will be of use to researchers developing new therapeutics for application in cystic fibrosis and beyond.
Design of the Class-E Power Amplifier with Finite DC Feed Inductance under Maximum-Rating Constraints
Authors: Casallas Ingrid , Paez-Rueda Carlos-Ivan , Perilla Gabriel , Pérez Manuel , Fajardo Arturo
Published: April 2021

This paper proposes an analytical expression set to determine the maximum values of currents and voltages in the Class-E Power Amplifier (PA) with Finite DC-Feed Inductance (FDI) under the following assumptions—ideal components (e.g., inductors and capacitors with infinite quality factor), a switch with zero rise and fall commutation times, zero on-resistance, and infinite off-resistance, and an infinite loaded quality factor of the output resonant circuit. The developed expressions are the average supply current, the RMS (Root Mean Square) current through the DC-feed inductance, the peak voltage and current in the switch, the RMS current through the switch, the peak voltages of the output resonant circuit, and the peak voltage and current in the PA load. These equations were obtained from the circuit analysis of this ideal amplifier and curve-fitting tools. Furthermore, the proposed expressions are a useful tool to estimate the maximum ratings of the amplifier components. The accuracy of the expressions was analyzed by the circuit simulation of twelve ideal amplifiers, which were designed to meet a wide spectrum of application scenarios. The resulting Mean Absolute Percentage Error (MAPE) of the maximum-rating constraints estimation was 2.64%.
PC12 and THP-1 Cell Lines as Neuronal and Microglia Model in Neurobiological Research
Authors: Balon Katarzyna , Wiatrak Benita
Published: April 2021

Models based on cell cultures have become a useful tool in modern scientific research. Since primary cell lines are difficult to obtain and handle, neoplasm-derived lines like PC12 and THP-1 offer a cheap and flexible solution for neurobiological studies but require prior differentiation to serve as a neuronal or microglia model. PC12 cells constitute a suitable research model only after differentiation by incubation with nerve growth factor (NGF) and THP-1 cells after administering a differentiation factor such as phorbol 12-myristate-13-acetate (PMA). Still, quite often, studies are performed on these cancer cells without differentiation. The study aimed to assess the impact of PC12 or THP-1 cell differentiation on sensitivity to harmful factors such as Aβ25-35 (0.001–5 µM) (considered as one of the major detrimental factors in the pathophysiology of Alzheimer’s disease) or lipopolysaccharide (1–100 µM) (LPS; a pro-inflammatory factor of bacterial origin). Results showed that in most of the tests performed, the response of PC12 and THP-1 cells induced to differentiation varied significantly from the effect in undifferentiated cells. In general, differentiated cells showed greater sensitivity to harmful factors in terms of metabolic activity and DNA damage, while in the case of the free radicals, the results were heterogeneous. Obtained data emphasize the importance of proper differentiation of cell lines of neoplastic origin in neurobiological research and standardization of cell culture handling protocols to ensure reliable results.
Comparison of Different Analytical Methods for the On-Site Analysis of Traces at Clandestine Drug Laboratories
Authors: Reiss René , Hauser Frank , Ehlert Sven , Pütz Michael , Zimmermann Ralf
Published: April 2021

While fast and reliable analytical results are crucial for first responders to make adequate decisions, these can be difficult to establish, especially at large-scale clandestine laboratories. To overcome this issue, multiple techniques at different levels of complexity are available. In addition to the level of complexity their information value differs as well. Within this publication, a comparison between three techniques that can be applied for on-site analysis is performed. These techniques range from ones with a simple yes or no response to sophisticated ones that allows to receive complex information about a sample. The three evaluated techniques are immunoassay drug tests representing easy to handle and fast to explain systems, ion mobility spectrometry as state-of-the-art equipment that needs training and experience prior to use and ambient pressure laser desorption with the need for a highly skilled operator as possible future technique that is currently under development. In addition to the measurement of validation parameters, real case samples are investigated to obtain practically relevant information about the capabilities and limitations of these techniques for on-site operations. Results demonstrate that in general all techniques deliver valid results, but the bandwidth of information widely varies between the investigated techniques.
Evaluation of the Perspective of ERA-Interim and ERA5 Reanalyses for Calculation of Drought Indicators for Uzbekistan
Authors: Rakhmatova Natella , Arushanov Mikhail , Shardakova Lyudmila , Nishonov Bakhriddin , Taryannikova Raisa , Rakhmatova Valeriya , Belikov Dmitry A.
Published: April 2021

The arid and semiarid regions of Uzbekistan are sensitive and vulnerable to climate change. However, the sparse and very unevenly distributed meteorological stations within the region provide limited data for studying the region’s climate variation. The aim of this work was to evaluate the performance of the European Centre for Medium-Range Weather Forecasts (ECMWF) Re-Analysis (ERA)-Interim and ERA5 products for the fields of near-surface temperature, humidity, and precipitation over Uzbekistan from 1981 to 2018 using observations from 74 meteorological stations. Major results suggested that the reanalysis datasets match well with most of the observed climate records, especially in the plain areas. While ERA5, with a high spatial resolution of 0.1°, is able more accurately reproduce mountain ranges and valleys. Compared to ERA-Interim, the climatological biases in temperature, humidity, and total precipitation from ERA5 are clearly reduced, and the representation of inter-annual variability is improved over most regions of Uzbekistan. Both reanalyses show a high level of agreement with observations on the standardized precipitation evaporation index (SPEI) with a correlation coefficient of 0.7–0.8.Although both of these ECMWF products can be successfully implemented for the calculation of atmospheric drought indicators for Uzbekistan and adjacent regions of Central Asia, the newer and advanced ERA5 is preferred.
Exploring the Relationships of Atmospheric Water Vapor Contents and Different Land Surfaces in a Complex Terrain Area by Using Doppler Radar
Authors: Lou Hezhen , Zhang Jun , Yang Shengtian , Cai Mingyong , Ren Xiaoyu , Luo Ya , Li Chaojun
Published: April 2021

Changes in atmospheric water vapor mainly occur in the atmospheric boundary layer. However, due to many factors, such as orography and ground thermal dynamic conditions, the change trends and transformation law of atmospheric water vapor contents above different surfaces are still unclear. In this work, a Doppler weather radar with high spatial-temporal resolution was used to monitor the variations and transformations of water vapor contents over different land surfaces for two years. The results show that the atmospheric water vapor content shows a very good positive correlation with elevation at altitudes between 600 m and 1200 m, while different land surfaces have delicate impacts on atmospheric water vapor contents, such as extreme values appearing above impervious urban surfaces, uniform distributions appearing over water body and vegetated surfaces being wet but avoiding extreme conditions. Compared with previous studies, the results and conclusions of this study are mainly derived from accurate direct observations based on high-resolution radar. Identifying the distribution and transformation of water vapor over different surfaces can enhance our understanding of the movement and variation of atmospheric water vapor over complex terrain and different land surfaces, and improve the planning and construction capacity of different surfaces, such that humankind can mitigate the severe disasters caused by drastic changes in atmospheric water vapor.
Role of Haptoglobin as a Marker of Muscular Improvement in Patients with Multiple Sclerosis after Administration of Epigallocatechin Gallate and Increase of Beta-Hydroxybutyrate in the Blood: A Pilot Study
Authors: de la Rubia Ortí Jose Enrique , Platero Jose Luis , Benlloch María , Franco-Martinez Lorena , Tvarijonaviciute Asta , Escribá-Alepuz Jesús , Sancho-Castillo Sandra
Published: April 2021

Here, we report on the role of haptoglobin (Hp), whose expression depends on the synthesis of interleukin 6 (IL-6), related to the pathogenesis of multiple sclerosis (MS), as a possible marker of muscle improvement achieved after treatment with the polyphenol epigallocatechin gallate (EGCG) and an increase in the ketone body beta-hydroxybutyrate (BHB) in the blood. After 4 months of intervention with 27 MS patients, we observed that Hp does not significantly increase, alongside a significant decrease in IL-6 and a significant increase in muscle percentage. At the same time, Hp synthesis is considerably and positively correlated with IL-6 both before and after treatment; while this correlation occurs significantly reversed with muscle percentage before treatment, no correlation is evident after the intervention. These results seem to indicate that Hp could be a marker of muscle status and could be a diagnosis tool after therapeutic intervention in MS patients.
Laboratory Monitoring of Direct Oral Anticoagulants (DOACs)
Authors: Dunois Claire
Published: April 2021

The introduction of direct oral anticoagulants (DOACs), such as dabigatran, rivaroxaban, apixaban, edoxaban, and betrixaban, provides safe and effective alternative to previous anticoagulant therapies. DOACs directly, selectively, and reversibly inhibit factors IIa or Xa. The coagulation effect follows the plasma concentration–time profile of the respective anticoagulant. The short half-life of a DOAC constrains the daily oral intake. Because DOACs have predictable pharmacokinetic and pharmacodynamic responses at a fixed dose, they do not require monitoring. However in specific clinical situations and for particular patient populations, testing may be helpful for patient management. The effect of DOACs on the screening coagulation assays such as prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin time (TT) is directly linked to reagent composition, and clotting time can be different from reagent to reagent, depending on the DOAC’s reagent sensitivity. Liquid chromatography–mass spectrometry (LC-MS/MS) is considered the gold standard method for DOAC measurement, but it is time consuming and requires expensive equipment. The general consensus for the assessment of a DOAC is clotting or chromogenic assays using specific standard calibrators and controls. This review provides a short summary of DOAC properties and an update on laboratory methods for measuring DOACs.
Carbon Dots as Promising Tools for Cancer Diagnosis and Therapy
Authors: Nocito Giuseppe , Calabrese Giovanna , Forte Stefano , Petralia Salvatore , Puglisi Caterina , Campolo Michela , Esposito Emanuela , Conoci Sabrina
Published: April 2021

Carbon Dots (CDs) are the latest members of carbon-based nanomaterials, which since their discovery have attracted notable attention due to their chemical and mechanical properties, brilliant fluorescence, high photostability, and good biocompatibility. Together with the ease and affordable preparation costs, these intrinsic features make CDs the most promising nanomaterials for multiple applications in the biological field, such as bioimaging, biotherapy, and gene/drug delivery. This review will illustrate the most recent applications of CDs in the biomedical field, focusing on their biocompatibility, fluorescence, low cytotoxicity, cellular uptake, and theranostic properties to highlight above all their usefulness as a promising tool for cancer diagnosis and therapy.
Calcium Signaling in the Thyroid: Friend and Foe
Authors: Asghar Muhammad Yasir , Lassila Taru , Törnquist Kid
Published: April 2021

Calcium signaling participates in a vast number of cellular processes, ranging from the regulation of muscle contraction, cell proliferation, and mitochondrial function, to the regulation of the membrane potential in cells. The actions of calcium signaling are, thus, of great physiological significance for the normal functioning of our cells. However, many of the processes that are regulated by calcium, including cell movement and proliferation, are important in the progression of cancer. In the normal thyroid, calcium signaling plays an important role, and evidence is also being gathered showing that calcium signaling participates in the progression of thyroid cancer. This review will summarize what we know in regard to calcium signaling in the normal thyroid as, well as in thyroid cancer.
Machine Learning for Prediction of Survival Outcomes with Immune-Checkpoint Inhibitors in Urothelial Cancer
Authors: Abuhelwa Ahmad Y. , Kichenadasse Ganessan , McKinnon Ross A. , Rowland Andrew , Hopkins Ashley M. , Sorich Michael J.
Published: April 2021

Machine learning (ML) may enhance the efficiency of developing accurate prediction models for survival, which is critical in informing disease prognosis and care planning. This study aimed to develop an ML prediction model for survival outcomes in patients with urothelial cancer-initiating atezolizumab and to compare model performances when built using an expert-selected (curated) versus an all-in list (uncurated) of variables. Gradient-boosted machine (GBM), random forest, Cox-boosted, and penalised, generalised linear models (GLM) were evaluated for predicting overall survival (OS) and progression-free survival (PFS) outcomes. C-statistic (c) was utilised to evaluate model performance. The atezolizumab cohort in IMvigor210 was used for model training, and IMvigor211 was used for external model validation. The curated list consisted of 23 pretreatment factors, while the all-in list consisted of 75. Using the best-performing model, patients were stratified into risk tertiles. Kaplan–Meier analysis was used to estimate survival probabilities. On external validation, the curated list GBM model provided slightly higher OS discrimination (c = 0.71) than that of the random forest (c = 0.70), CoxBoost (c = 0.70), and GLM (c = 0.69) models. All models were equivalent in predicting PFS (c = 0.62). Expansion to the uncurated list was associated with worse OS discrimination (GBM c = 0.70; random forest c = 0.69; CoxBoost c = 0.69, and GLM c = 0.69). In the atezolizumab IMvigor211 cohort, the curated list GBM model discriminated 1-year OS probabilities for the low-, intermediate-, and high-risk groups at 66%, 40%, and 12%, respectively. The ML model discriminated urothelial-cancer patients with distinctly different survival risks, with the GBM applied to a curated list attaining the highest performance. Expansion to an all-in approach may harm model performance.
Encapsulated NOLA™ Fit 5500 Lactase—An Economically Beneficial Way to Obtain Lactose-Free Milk at Low Temperature
Authors: Czyzewska Katarzyna , Trusek Anna
Published: April 2021

The current requirements of industrial biocatalysis are related to economically beneficial and environmentally friendly processes. Such a strategy engages low-temperature reactions. The presented approach is essential, especially in food processes, where temperature affects the quality and nutritional value foodstuffs. The subject of the study is the hydrolysis of lactose with the commercial lactase NOLA™ Fit 5500 (NOLA). The complete decomposition of lactose into two monosaccharides gives a sweeter product, recommended for lactose intolerant people and those controlling a product’s caloric content. The hydrolysis reaction was performed at 15 °C, which is related to milk transportation and storage temperature. The enzyme showed activity over the entire range of substrate concentrations (up to 55 g/L lactose). For reusability and easy isolation, the enzyme was encapsulated in a sodium alginate network. Its stability allows carrying out six cycles of the complete hydrolysis of lactose to monosaccharides, lasting from two to four hours. During the study, the kinetic description of native and encapsulated NOLA was conducted. As a result, the model of competitive galactose inhibition and glucose mixed influence (competitive inhibition and activation) was proposed. The capsule size does not influence the reaction rate; thus, the substrate diffusion into capsules can be omitted from the process description. The prepared 4 mm capsules are easy to separate between cycles, e.g., using sieves.
Desquamation in Kawasaki Disease
Authors: Chang Ling-Sai , Weng Ken-Pen , Yan Jia-Huei , Lo Wan-Shan , Guo Mindy Ming-Huey , Huang Ying-Hsien , Kuo Ho-Chang
Published: April 2021

(1) Background: Desquamation is a common characteristic of Kawasaki disease (KD). In this study, we analyzed patients’ varying desquamation levels in their hands or feet, in correlation with clinical presentation, to assess the relationship. (2) Methods: We retrospectively reviewed children with KD. We analyzed their age, laboratory data before intravenous immunoglobulin (IVIG) treatment and coronary artery abnormalities (CAA) based on the desquamation level of their hands and feet. We classified the desquamation level from 0 to 3 and defined high-grade desquamation as grade 2 and 3. (3) Results: We enrolled a total 112 patients in the study. We found the hands’ high-grade desquamation was positively associated with age and segmented neutrophil percentage (p = 0.047 and 0.029, respectively) but negatively associated with lymphocyte and monocyte percentage (p = 0.03 and 0.006, respectively). Meanwhile, the feet’s high-grade desquamation was positively associated with total white blood cell counts (p = 0.033). Furthermore, we found that high-grade hand desquamation had less probability of CAA formation compared with that of a low grade (7.1% vs. 40.8%, p = 0.016). (4) Conclusions: This report is the first to demonstrate that the desquamation level of hands or feet in KD is associated with different coronary artery abnormalities and laboratory findings.
Interface between Periodontal Tissues and Dental Materials: Dynamic Changes and Challenges
Authors: Isola Gaetano
Published: April 2021

Periodontal tissues play an important role in the correct aesthetics, function and comfort of the dentition [...]
Thermal Performance of Structural Lightweight Concrete Composites for Potential Energy Saving
Authors: Lee Yeong Huei , Chua Nicholas , Amran Mugahed , Yong Lee Yee , Hong Kueh Ahmad Beng , Fediuk Roman , Vatin Nikolai , Vasilev Yuriy
Published: April 2021

Residential consumption dominates the energy expenditure of heating and cooling systems, especially in tropical climates where building envelopes play an important role in energy efficiency. The thermal properties of concrete that are commonly employed as the building envelope material affect directly human comfort in a building. In addressing both the concrete thermal performance and industrial waste issues, this paper experimentally studies the concrete compressive strength and thermal properties used later for comparative energy analysis for human comfort. Four design mixes and a conventional concrete as control specimen are considered utilizing industrial wastes; palm oil fly ash (POFA), lightweight expanded clay aggregate (LECA), oil palm shell (OPS), and quarry dust, as constituents. These mixes are cast for cube compressive strength (to ensure the achievement of structural concrete requirement) and small-scaled wall tests. The measurement of surface temperatures of scaled wall tests is conducted in a polystyrene box to determine the concrete time lag and decrement factor. It is found that the density of concrete governs the compressive strength and that air pockets in the concrete matrix play an essential role as far as the thermal properties are concerned. From the energy analysis, structural lightweight concrete may save approximately 50% of the residential energy consumption.
The Introduction of the Asian Red Algae Melanothamnus japonicus (Harvey) Díaz-Tapia & Maggs in Peru as a Means to Adopt Management Strategies to Reduce Invasive Non-Indigenous Species
Authors: Sánchez-Velásquez Julissa J. , Reyes-Flores Lorenzo E. , Yzásiga-Barrera Carmen , Zelada-Mázmela Eliana
Published: April 2021

Early detection of non-indigenous species is crucial to reduce, mitigate, and manage their impacts on the ecosystems into which they were introduced. However, assessment frameworks for identifying introduced species on the Pacific Coast of South America are scarce and even non-existent for certain countries. In order to identify species’ boundaries and to determine the presence of non-native species, through morphological examinations and the analysis of the plastid ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL-5P) gene, we investigated the phylogenetic relationships among species of the class Florideophyceae from the coast of Ancash, Peru. The rbcL-5P dataset revealed 10 Florideophyceae species distributed in the following four orders: Gigartinales, Ceramiales, Halymeniales, and Corallinales, among which the Asian species, Melanothamnus japonicus (Harvey) Díaz-Tapia & Maggs was identified. M. japonicus showed a pairwise divergence of 0% with sequences of M. japonicus from South Korea, the USA, and Italy, the latter two being countries where M. japonicus has been reported as introduced species. Our data indicate a recent introduction event of M. japonicus in Peru, and consequently, the extension of its distribution into South America. These findings could help to adopt management strategies for reducing the spread and impact of M. japonicus on the Pacific Coast of South America.
Assessing Baveno VI Criteria Using Liver Stiffness Measured with a 2D-Shear Wave Elastography Technique
Authors: Fofiu Renata , Bende Felix , Popescu Alina , Șirli Roxana , Miuţescu Bogdan , Sporea Ioan
Published: April 2021

The present study evaluates the performance of Baveno VI criteria, using liver stiffness (LS) assessed with a 2D-SWE elastography technique, for predicting high-risk varices (HRV) in patients with compensated advanced chronic liver disease (cACLD). A secondary aim was to determine whether the use of spleen stiffness measurements (SSMs), as additional criteria, increases the performance of the 2D-SWE Baveno VI criteria. Data were collected from 208 subjects with cACLD, who underwent abdominal ultrasound, liver and spleen stiffness measurements, and upper digestive endoscopy. HRV were defined as grade 1 esophageal varices (EV) with red wale marks, grade 2/3 EV, and gastric varices. A total of 35.6% (74/208) of the included subjects had HRV. The optimal LS cut-off value for predicting HRV was 12 kPa (AUROC-0.80). Using both LS cut-off value < 12 kPa and a platelet cut-off value > 150 × 109 cells/L as criteria to exclude HRV, 52/208 (25%) subjects were selected, 88.5% (46/52) were without EV, 9.6% (5/52) had grade 1 EV, and 1.9% (1/52) had HRV. Thus 98% of the subjects were correctly classified as having or not having HRV and 25% of the surveillance endoscopies could have been avoided. Using SS < 13.2 kPa and a platelet cut-off value > 150 × 109 cells/L as additional criteria for the patients that were outside the initial ones, 32.7% of the surveillance endoscopies could have been avoided.
Obesity Drives an Oral Microbiota Signature of Female Patients with Periodontitis: A Pilot Study
Authors: Thomas Charlotte , Minty Matthieu , Canceill Thibault , Loubières Pascale , Azalbert Vincent , Tercé François , Champion Camille , Burcelin Rémy , Barthet Pierre , Laurencin-Dalicieux Sara , Blasco-Baque Vincent
Published: April 2021

The aim of this study was to analyze the link between oral microbiota and obesity in humans. We conducted a pilot study including 19 subjects with periodontitis divided into two groups: normo-weighted subjects (NWS) with a body mass index (BMI) between 20 and 25 (n = 9) and obese subjects (OS) with a BMI > 30 (n = 10). Obesity was associated with a poor oral health status characterized by an increased number of missing teeth and a higher score of periodontal-support loss associated with dysbiotic oral microbiota (39.45 ± 3.74 vs. 26.41 ± 11.21, p = 0.03 for the Chao 1 index). Oral microbiota taxonomic analysis showed that the abundance of the Capnocytophaga genus was higher (2.47% ± 3.02 vs. 0.27% ± 0.29, p = 0.04) in OS compared to NWS. Obese females (OF) were characterized by an increase in the Streptococcus genus (34.12% ± 14.29 vs. 10.55% ± 10.42, p = 0.05) compared to obese males (OM), where the Neisseria genus was increased (5.75% ± 5.03 vs. 58.05% ± 30.64, p = 0.008). These first data suggest that sex/gender is determinant in the link between oral dysbiotic microbiota and obesity in patients with periodontitis. Our results could lead to recommendations concerning therapeutic strategies for obese patients with periodontitis following the sex/gender.