bluuebunny/crossref_metadata_2025 huggingface dataset:
Droughts Are Not the Likely Primary Cause for Abies sibirica and Pinus sibirica Forest Dieback in the South Siberian Mountains
Authors: Tchebakova Nadezhda M. , Parfenova Elena I. , Bazhina Elena V. , Soja Amber J. , Groisman Pavel Ya.
Published: August 2022

Background. Since the mid-20th century, massive dieback of coniferous forests has been observed in the temperate and boreal zones across North America and Northern Eurasia. The first hypotheses explaining forest dieback were associated with industrial air pollution (acid rain). At the end of the century, new hypotheses emerged that supported critical climate-induced aridization to explain forest dieback. Many studies were based on the SPEI (Standardized Precipitation Evapotranspiration Index) drought index. Our goals were to investigate if the SPEI drought index was a suitable metric to reflect drought conditions in wet and moist dark-needled forests in the South Siberian Mountains (Mts) and if droughts trigger the dieback of those forests. Methods. We calculated the SPEI drought index, the annual moisture index AMI, potential evapotranspiration PET, and water balance dynamics for the period 1961–2019 for four transects in the South Siberian Mts. where decline/dieback of dark-needled Siberian pine and fir forests were identified in situ. Climate data from nine weather stations located at lower and upper elevations of each transect were used to calculate climatic index dynamics for the 1961–2019 period to identify dry and wet phases of the period. Results. Our findings showed that climatic moisture/dryness indices have rarely gone down to high risk levels during the last 60 years (1961–2019). AMI did not reach the critical limit, 2.25, characteristic of the lower border for the dark-needled taiga. SPEI values < −1.5 represent drought stress conditions for dark-needled conifers at the lower border, and these conditions occurred 3–4 times during the 60-year period. However, the annual water balance stayed positive in those years in wet and moist forests at mid-to-high elevations. Trees are known to survive occasional (1–2) dry years. We found that dark-needled conifer dieback often occurs in wet years with plentiful rain rather than in drought years. We found forest dieback was associated with the westerlies that bring atmospheric pollution from the west at 50–56 N latitudes, where the air masses cross populated regions that have widespread industrial complexes. Conclusions. We concluded that the observed decline of dark-needled conifers at middle-to-high elevations across the South Siberia’s Mts was conditioned by several plausible causes, among which air pollution seems to be more credible, rather than dry climatic conditions, as cited in recent literature. Results are essential for understanding these ecosystems and others as our planet changes. Other causes and mechanisms should be further investigated, which would necessitate creating infrastructure that supports multi-disciplinary, inter-agency teamwork of plant physiologists, foresters, chemists, etc.
Nondestructive Techniques for Determination of Wood Mechanical Properties of Urban Trees in Madrid
Authors: Virgen-Cobos Gabriel H. , Olvera-Licona Guadalupe , Hermoso Eva , Esteban Miguel
Published: August 2022

The aim of this paper is to analyze the mechanical properties of Robinia pseudoacacia L. (black locust), Platanus × hybrida Brot. (London plane), Ulmus pumila L. (Siberian elm), and Populus alba L. (white poplar), estimated using ultrasound wave equipment (USLab y Sylvatest Duo) and impact wave equipment (Microsecond Timer) on standing trees, felled logs and on specimens obtained from different parts of the trees. Bending strength, axial compression strength and tension parallel to grain were determined for each specimen, in order to subsequently correlate the strength and stiffness with the acoustic properties determined in the standing tree. For the relationship between the static modulus of elasticity of the specimens and the dynamic modulus of elasticity determined in standing trees using the USLab, coefficients of determination were found to be between 0.30–0.92, between 0.52 and 0.80 using the Sylvatest Duo and between 0.60 and 0.94 with the Microsecond Timer. It would be necessary to determine whether the removal of bark for the correct coupling of the ultrasonic sensors would provide an entry route for pests or diseases that could affect the health of the tree.
Multiple Positive Solutions for Fractional Boundary Value Problems with Integral Boundary Conditions and Parameter Dependence
Authors: Tabti Hamza , Belmekki Mohammed
Published: August 2022

In this paper, we consider the existence of multiple positive solutions to boundary value problems of nonlinear fractional differential equation with integral boundary conditions and parameter dependence. To obtain our results, we used a functional-type cone expansion-compression fixed point theorem and the Leggett–Williams fixed point theorem. Examples are included to illustrate the main results.
In Vivo Effect of Resveratrol-Cellulose Aerogel Drug Delivery System to Relieve Inflammation on Sports Osteoarthritis
Authors: Cui Ningxin , Xu Zhen , Zhao Xinyu , Yuan Meng , Pan Leiyu , Lu Tianfeng , Du Ai , Qin Lili
Published: August 2022

Resveratrol (Res) is a plant extract with strong anti-inflammatory, antioxidant and anti-aging biological activities. However, Res is limited by its disadvantages, such as poor solubility, rapid metabolism and low bioavailability. In this study, the Resveratrol-loaded TEMPO-oxidized cellulose aerogel (RLTA) drug delivery system was prepared by the method of “dissolution-freeze-drying” and characterized by a series of analysis. Then the blood biochemical indexes and HE staining were measured and analyzed in animal experiments. The in vivo results showed that RLTA can decrease the levels of TNF-α and IL-6 inflammatory factors in the synovial fluid. Furthermore, the molecular mechanism was investigated through the analysis of silent information regulator 2 homolog 1 (Sirt1) protein expression, which suggested that RLTA could upregulate the expression of Sirt1 and mediate the P38 signaling pathway, thereby inhibiting the expression of COX-2 and MMP13 which can suppress the levels of IL-6 and TNF-α inflammatory factor. These results reveal that cellulose aerogel is a promising candidate for drug delivery and RLTA has great potential application for the treatment of sports osteoarthritis.
Nano-Modified Polymer Gels as Temperature- and Salt-Resistant Fluid-Loss Additive for Water-Based Drilling Fluids
Authors: Li Jian , Sun Jinsheng , Lv Kaihe , Ji Yuxi , Ji Jintao , Liu Jingping
Published: August 2022

With the continuous exploration and development of oil and gas resources to deep formations, the key treatment agents of water-based drilling fluids face severe challenges from high temperatures and salinity, and the development of high temperature and salt resistance filtration reducers has always been the focus of research in the field of oilfield chemistry. In this study, a nano-silica-modified co-polymer (NS-ANAD) gel was synthesized by using acrylamide, isopropylacrylamide, 2-acrylamide-2-methyl propane sulfonic acid, diallyl dimethyl ammonium chloride, and double-bond-modified inorganic silica particles (KH570-SiO2) through free radical co-polymerization. The introduction of nanotechnology enhances the polymer’s resistance to high temperature degradation, making it useful as a high-temperature-resistant fluid loss reducer. Moreover, the anions (sulfonates) and cations (quaternary ammonium) enhance the extension of the polymer and the adsorption on the surface of bentonite particles in a saline environment, which in turn improves the salt resistance of the polymer. The drilling fluids containing 2.0 wt% NS-ANAD co-polymer gels still show excellent rheological and filtration performance, even after aging in high temperature (200 °C) and high salinity (saturated salt) environments, showing great potential for application in deep and ultra-deep drilling engineering.
The Multi-Omic Landscape of Primary Breast Tumors and Their Metastases: Expanding the Efficacy of Actionable Therapeutic Targets
Authors: Yang Guang , Lu Tao , Weisenberger Daniel J. , Liang Gangning
Published: August 2022

Breast cancer (BC) mortality is almost exclusively due to metastasis, which is the least understood aspect of cancer biology and represents a significant clinical challenge. Although we have witnessed tremendous advancements in the treatment for metastatic breast cancer (mBC), treatment resistance inevitably occurs in most patients. Recently, efforts in characterizing mBC revealed distinctive genomic, epigenomic and transcriptomic (multi-omic) landscapes to that of the primary tumor. Understanding of the molecular underpinnings of mBC is key to understanding resistance to therapy and the development of novel treatment options. This review summarizes the differential molecular landscapes of BC and mBC, provides insights into the genomic heterogeneity of mBC and highlights the therapeutically relevant, multi-omic features that may serve as novel therapeutic targets for mBC patients.
Application of Feature Selection and Deep Learning for Cancer Prediction Using DNA Methylation Markers
Authors: Gomes Rahul , Paul Nijhum , He Nichol , Huber Aaron Francis , Jansen Rick J.
Published: August 2022

DNA methylation is a process that can affect gene accessibility and therefore gene expression. In this study, a machine learning pipeline is proposed for the prediction of breast cancer and the identification of significant genes that contribute to the prediction. The current study utilized breast cancer methylation data from The Cancer Genome Atlas (TCGA), specifically the TCGA-BRCA dataset. Feature engineering techniques have been utilized to reduce data volume and make deep learning scalable. A comparative analysis of the proposed approach on Illumina 27K and 450K methylation data reveals that deep learning methodologies for cancer prediction can be coupled with feature selection models to enhance prediction accuracy. Prediction using 450K methylation markers can be accomplished in less than 13 s with an accuracy of 98.75%. Of the list of 685 genes in the feature selected 27K dataset, 578 were mapped to Ensemble Gene IDs. This reduced set was significantly (FDR < 0.05) enriched in five biological processes and one molecular function. Of the list of 1572 genes in the feature selected 450K data set, 1290 were mapped to Ensemble Gene IDs. This reduced set was significantly (FDR < 0.05) enriched in 95 biological processes and 17 molecular functions. Seven oncogene/tumor suppressor genes were common between the 27K and 450K feature selected gene sets. These genes were RTN4IP1, MYO18B, ANP32A, BRF1, SETBP1, NTRK1, and IGF2R. Our bioinformatics deep learning workflow, incorporating imputation and data balancing methods, is able to identify important methylation markers related to functionally important genes in breast cancer with high accuracy compared to deep learning or statistical models alone.
Prevalence of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae in Asymptomatic Women from Urban-Peripheral and Rural Populations of Cuenca, Ecuador
Authors: Abad Sebastián , Neira Elizavet , Viñansaca Lourdes , Escandón Samuel , Neira Vivian Alejandra
Published: August 2022

Background: Sexually transmitted diseases (STDs) are a serious public health issue due to their high prevalence and a substantial percentage of women being asymptomatic. The present study aimed to determine the prevalence of three STD-causative pathogens in asymptomatic women from Southern Ecuador, with the ultimate purpose of updating the epidemiological data and obtaining a timely diagnosis, which can prevent further complications. Methods: This cross-sectional study included 102 asymptomatic women from Cuenca, Ecuador, who underwent a cervical cytology examination. They met all the inclusion criteria and signed the consent form. Nucleic acids were extracted from each sample, and PCR and flow-through hybridization were performed to detect the pathogens responsible for three STDs. Descriptive and inferential statistics were used to define and describe the study population, obtain the frequency data, and measure central tendencies to determine possible associations among the variables. Results: We found that 49.02% of the participants were infected with at least one of the three microorganisms, with 48.04% and 2.94% carrying Ureaplasma urealyticum (UU) and Chlamydia trachomatis (CT), respectively. Neisseria gonorrhoeae (NG) infection was not observed. Among the participants, 1.96% presented co-infections with CT and UU. Approximately half of the participants presented with asymptomatic infections caused by at least one microorganism. Conclusions: This study demonstrates the importance of conducting regular STD screening programs for high-risk asymptomatic women.
Genome-Wide Analysis of Odorant and Gustatory Receptors in Six Papilio Butterflies (Lepidoptera: Papilionidae)
Authors: Yin Ningna , Xiao Haiyan , Yang Anjin , Wu Chun , Liu Naiyong
Published: August 2022

The chemical interactions of insects and host plants are shaping the evolution of chemosensory receptor gene families. However, the correlation between host range and chemoreceptor gene repertoire sizes is still elusive in Papilionidae. Here, we addressed the issue of whether host plant diversities are correlated with the expansions of odorant (ORs) or gustatory (GRs) receptors in six Papilio butterflies. By combining genomics, transcriptomics and bioinformatics approaches, 381 ORs and 328 GRs were annotated in the genomes of a generalist P. glaucus and five specialists, P. xuthus, P. polytes, P. memnon, P. machaon and P. dardanus. Orthologous ORs or GRs in Papilio had highly conserved gene structure. Five Papilio specialists exhibited a similar frequency of intron lengths for ORs or GRs, but which was different from those in the generalist. Phylogenetic analysis revealed 60 orthologous OR groups, 45 of which shared one-to-one relationships. Such a single gene in each butterfly also occurred in 26 GR groups. Intriguingly, bitter GRs had fewer introns than other GRs and clustered into a large clade. Focusing on the two chemoreceptor gene families in P. xuthus, most PxutORs (52/58) were expressed in antennae and 31 genes in reproductive tissues. Eleven out of 28 foretarsus-expressed PxutGRs were female-biased genes, as strong candidates for sensing oviposition stimulants. These results indicate that the host range may not shape the large-scale expansions of ORs and GRs in Papilio butterflies and identify important molecular targets involved in olfaction, oviposition or reproduction in P. xuthus.
Angle-Retaining Chromaticity and Color Space: Invariants and Properties
Authors: Buzzelli Marco
Published: August 2022

The angle-retaining color space (ARC) and the corresponding chromaticity diagram encode information following a cylindrical color model. Their main property is that angular distances in RGB are mapped into Euclidean distances in the ARC chromatic components, making the color space suitable for data representation in the domain of color constancy. In this paper, we present an in-depth analysis of various properties of ARC: we document the variations in the numerical precisions of two alternative formulations of the ARC-to-RGB transformation and characterize how various perturbations in RGB impact the ARC representation. This was done empirically for the ARC diagram in a direct comparison against other commonly used chromaticity diagrams, and analytically for the ARC space with respect to its three components. We conclude by describing the color space in terms of perceptual uniformity, suggesting the need for new perceptual color metrics.
Using Polygenic Risk Scores Related to Complex Traits to Predict Production Performance in Cross-Breeding of Yeast
Authors: Dai Yi , Shi Guohui , Chen Mengmeng , Chen Guotao , Wu Qi
Published: August 2022

The cultivation of hybrids with favorable complex traits is one of the important goals for animal, plant, and microbial breeding practices. A method that can closely predict the production performance of hybrids is of great significance for research and practice. In our study, polygenic risk scores (PRSs) were introduced to estimate the production performance of Saccharomyces cerevisiae. The genetic variation of 971 published isolates and their growth ratios under 35 medium conditions were analyzed by genome-wide association analysis, and the precise p-value threshold for each phenotype was calculated. Risk markers for the above 35 phenotypes were obtained. By estimating the genotype of F1 hybrids according to that of the parents, the PRS of 613 F1 hybrids was predicted. There was a significant linear correlation between the maximum growth rate at 40 °C and PRS in F1 hybrids and their parents (R2 = 0.2582, R2 = 0.2414, respectively), which indicates that PRS can be used to estimate the production performance of individuals and their hybrids. Our method can provide a reference for strain selection and F1 prediction in cross-breeding yeasts, reduce workload, and improve work efficiency.
Activated Sintering of Cr2O3-Based Composites by Hot Pressing
Authors: Gevorkyan Edwin , Cepova Lenka , Rucki Mirosław , Nerubatskyi Volodymyr , Morozow Dmitrij , Zurowski Wojciech , Barsamyan Voskan , Kouril Karel
Published: August 2022

The paper presents and discusses questions on structure formation during the sintering process of Cr2O3-based composites using the hot pressing method, when a chemical reaction between the components takes place. The task was difficult because Cr2O3 decomposes when sintered at temperatures above 1300 °C. The proposed novel method allowed for interaction between aluminum and chromia, thus avoiding the decomposition of the latter. Here, ultrafine aluminum powder played the role of the active agent forming a liquid phase and reacting with Cr2O3. The appearance of the solid solutions of (Cr,Al)2O3 with different stoichiometry of Cr and Al depended on the aluminum content in the initial mixture. The solid solution significantly strengthened boundaries between composite phases, resulting in the composite material of high fracture toughness between 5 and 7 MPa m½ and bending strength of ca. 500 MPa. The best mechanical properties exhibited the cermet with 22 wt.% of the restored chromium.
A Study on the Mechanism and Kinetics of Ultrasound-Enhanced Sulfuric Acid Leaching for Zinc Extraction from Zinc Oxide Dust
Authors: Zheng Xuemei , Li Shiwei , Liu Bingguo , Zhang Libo , Ma Aiyuan
Published: August 2022

As an important secondary zinc resource, large-scale reserves of zinc oxide dust (ZOD) from a wide range of sources is of high comprehensive recycling value. Therefore, an experimental study on ultrasound-enhanced sulfuric acid leaching for zinc extraction from zinc oxide dust was carried out to investigate the effects of various factors such as ultrasonic power, reaction time, sulfuric acid concentration, and liquid–solid ratio on zinc leaching rate. The results show that the zinc leaching rate under ultrasound reached 91.16% at a temperature of 25 °C, ultrasonic power 500 W, sulfuric acid concentration 140 g/L, liquid–solid ratio 5:1, rotating speed 100 r/min, and leaching time 30 min. Compared with the conventional leaching method (leaching rate: 85.36%), the method under ultrasound increased the zinc leaching rate by 5.8%. In a kinetic analysis of the ultrasound-enhanced sulfuric acid leaching of zinc oxide dust, the initial apparent activation energy of the reaction was 6.90 kJ/mol, indicating that the ultrasound-enhanced leaching process was controlled by the mixed solid product layers. Furthermore, the leached residue was characterized by XRD and SEM-EDS, and the results show that, with ultrasonic waves, the encapsulated mineral particles were dissociated, and the dissolution of ZnO was enhanced. Mostly, the zinc in leached residue existed in the forms of ZnFe2O4, Zn2SiO4, and ZnS.
An Investigation into a Miniature Saltless Solar Pond
Authors: El-Batouti Mervette , Naim Mona M. , Al Harby Nouf F. , Elewa Mahmoud M.
Published: August 2022

A simple, miniature saltless Solar Pond (SP) was designed and constructed in the present work. It consisted of a Plexiglas container with a square cross-section, within which cruciform baffles were suspended in the upper half of the pond, and copper coil tubing was fitted in the middle of the lower zone to function as a heat exchanger without disturbing the pond’s inertia. Different variables’ effect on the water’s temperature rise at various vertical locations within the pond were investigated. These variables included the presence of the cruciform baffles, the inclination of a mirror fixed to the top rim of the pond, a glass transparent cover (GC), and the presence or absence of a gel thickening material to increase the water viscosity inside the SP, the climatic conditions, and the presence of glass wool (GW) in the lower section of the SP. For an experiment, an estimated energy balance was performed, and the thermal storage efficiency was calculated. The best obtained thermal storage efficiency was 32.58% in the presence of the cruciform baffles, a 45° inclination of a mirror fixed to the top rim of the SP, at an ambient temperature of 30 °C on a calm, sunny day with a wind speed of 7 km/h.
New Organic-Inorganic Hybrid Compounds Based on Sodium Peroxidomolybdates (VI) and Derivatives of Pyridine Acids: Structure Determination and Catalytic Properties
Authors: Sławińska Adrianna , Tyszka-Czochara Malgorzata , Serda Paweł , Oszajca Marcin , Ruggiero-Mikołajczyk Małgorzata , Pamin Katarzyna , Napruszewska Bogna D. , Prochownik Ewelina , Łasocha Wiesław
Published: August 2022

Two organic-inorganic hybrids based on sodium peroxidomolybdates(VI) and 3,5-dicarboxylic pyridine acid (Na-35dcpa) or N-oxide isonicotinic acid (Na-isoO) have been synthesized and characterized. All compounds contain inorganic parts: a pentagonal bipyramid with molybdenum center, and an organic part containing 3,5-dicarboxylic pyridine acid or N-oxide isonicotinic acid moieties. The type of organic part used in the synthesis influences the crystal structure of obtained compounds. This aspect can be interesting for crystal engineering. Crystal structures were determined using powder X-ray diffraction or single crystal diffraction for compounds Na-35dcpa and Na-isoO, respectively. Elemental analysis was used to check the purity of the obtained compounds, while X-ray Powder Diffraction (XRPD) vs. temp. was applied to verify their stability. Moreover, all the compounds were examined by Infrared (IR) spectroscopy. Their catalytic activity was tested in the Baeyer–Villiger (BV) oxidation of cyclohexanone to ε-caprolactone in the oxygen-aldehyde system. The highest catalytic activity in the BV oxidation was observed for Na-35dcpa. The compounds were also tested for biological activity on human normal cells (fibroblasts) and colon cancer cell lines (HT-29, LoVo, SW 620, HCT 116). All compounds were cytotoxic against tumor cells with metastatic characteristics, which makes them interesting and promising candidates for further investigations of specific anticancer mechanisms.
Stability Analysis for Time-Delay Systems via a New Negativity Condition on Quadratic Functions
Authors: Xiao Shenping , Yu Jin , Yang Simon X. , Qiu Yongfeng
Published: August 2022

This article studies the stability problem of linear systems with time-varying delays. First, a new negative condition is established for a class of quadratic functions whose variable is within a closed set. Then, based on this new condition, a couple of stability criteria for the system under study are derived by constructing an appropriate Lyapunov–Krasovskii functional. Finally, it is demonstrated through two numerical examples that the proposed stability criteria are efficient and outperform some existing methods.
Extending Fuzzy Linguistic Logic Programming with Negation †
Authors: Le Van Hung
Published: August 2022

Fuzzy linguistic logic programming (FLLP) is a framework for representation and reasoning with linguistically expressed human knowledge. In this paper, we extend FLLP by allowing negative literals to appear in rule bodies, resulting in normal logic programs. We study the stable model semantics and well-founded semantics of such programs and their relation. The two kinds of semantics are adapted from those of classical ones based on the Gelfond–Lifschitz transformation and van Gelder’s alternating fixpoint approach, respectively. To our knowledge, until now, there has been no work on the well-founded semantics of normal programs in any fuzzy logic programming (FLP) framework based on Vojtáš’s FLP. Moreover, the relation between the two kinds of semantics is usually studied using a bilattice setting of the truth domain. However, our truth domains do not possess a complete knowledge-ordering lattice and, thus, do not have a bilattice structure. The two kinds of semantics possess properties similar to those of the classical case. Every stable model contains the well-founded (partial) model, and the well-founded total model coincides with the unique stable model, but not vice versa. Since the well-founded semantics is closely related to the stable model semantics, it can help compute stable models more efficiently.
Production of Antioxidants and High Value Biomass from Nannochloropsis oculata: Effects of pH, Temperature and Light Period in Batch Photobioreactors
Authors: Andriopoulos Vasilis , Lamari Fotini N. , Hatziantoniou Sophia , Kornaros Michael
Published: August 2022

Nannochloropsis oculata is a marine microalgal species with a great potential as food or feed due to its high pigment, protein and eicosapentaenoic acid contents. However, for such an application to be realized on a large scale, a biorefinery approach is necessary due to the high cost of microalgal biomass production. For example, techno economic analyses have suggested the co-production of food or feed with antioxidants, which can be extracted and supplied separately to the market. The aim of this study was to investigate the effect of cultivation conditions on the antioxidant capacity of Nannochlosopsis oculata extracts, derived with ultrasound-assisted extraction at room temperature, as well as the proximate composition and fatty acid profile of the biomass. A fractional factorial approach was applied to examine the effects of temperature (20–35 °C), pH (6.5–9.5) and light period (24:0, 12:12). At the end of each run, biomass was collected, washed with 0.5M ammonium bicarbonate and freeze-dried. Antioxidant capacity as gallic acid equivalents as well as pigment content were measured in the ethanolic extracts. Optimal conditions were different for productivity and biomass composition. Interesting results regarding the effect of light period (LP) and pH require further investigation, whereas the effect of moisture on the extraction process was confounded with biomass composition. Finally, further data is provided regarding the relation between chlorophyll content and apparent phenolic content using the Folin–Ciocalteu assay, in agreement with our previous work.
Echinochrome A Inhibits Melanogenesis in B16F10 Cells by Downregulating CREB Signaling
Authors: Choi Mi , Lee Heejin , Kim Hyoung , Han Jin , Seol Jung , Vasileva Elena , Mishchenko Natalia , Fedoreyev Sergey , Stonik Valentin , Ju Won , Kim Dai-Jin , Lee Sang-Rae
Published: August 2022

Excessive increase in melanin pigment in the skin can be caused by a variety of environmental factors, including UV radiation, and can result in spots, freckles, and skin cancer. Therefore, it is important to develop functional whitening cosmetic reagents that regulate melanogenesis. In this study, we investigated the effects of echinochrome A (Ech A) on melanogenesis in the B16F10 murine melanoma cell line. We triggered B16F10 cells using α-MSH under Ech A treatment to observe melanin synthesis and analyze expression changes in melanogenesis-related enzymes (tyrosinase, tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (TYRP2)) at the mRNA and protein levels. Furthermore, we measured expression changes in the microphthalmia-associated transcription factor (MITF), CREB, and pCREB proteins. Melanin synthesis in the cells stimulated by α-MSH was significantly reduced by Ech A. The expression of the tyrosinase, TYRP1, and TYRP2 mRNA and proteins was significantly decreased by Ech A, as was that of the MITF, CREB, and pCREB proteins. These results show that Ech A suppresses melanin synthesis by regulating melanogenesis-related enzymes through the CREB signaling pathway and suggest the potential of Ech A as a functional agent to prevent pigmentation and promote skin whitening.
Simulation and Experimental Investigation of the Vacuum-Enhanced Direct Membrane Distillation Driven by a Low-Grade Heat Source
Authors: Ma Qingfen , Tong Liang , Wang Chengpeng , Cao Guangfu , Lu Hui , Li Jingru , Liu Xuejin , Feng Xin , Wu Zhongye
Published: August 2022

Vacuum-enhanced direct contact membrane distillation (VEDCMD) has been proven experimentally to improve the permeate flux, compared with direct contact membrane distillation (DCMD). However, the theoretical mechanism for its transmembrane transfer process has not been revealed sufficiently. In this paper, with full consideration of the different driving forces of diffusion and Poiseuille flow under the vacuum enhancing condition, a theoretical transmembrane model for mass and heat transfer in VEDCMD is proposed. The CFD model and experimental platform are established to verify the theoretical model. The simulated results agree with the experimental data well, and nearly 200% improvement of the permeate flux is obtained when the permeate pressure drops to 30 kPa. The flow fields of the flow along the membrane surface are obtained and analyzed, with good consistency in the variation of the permeate flux. Since all the parameters of the proposed model are independent of the operating condition, the model is much easier for use and has better adaptability to fluctuating operating conditions.
A Multi-Matrix Metabolomic Approach in Ringed Seals and Beluga Whales to Evaluate Contaminant and Climate-Related Stressors
Authors: Simond Antoine É. , Noël Marie , Loseto Lisa , Houde Magali , Kirk Jane , Elliott Ashley , Brown Tanya M.
Published: August 2022

As a high trophic-level species, ringed seals (Pusa hispida) and beluga whales (Delphinapterus leucas) are particularly vulnerable to elevated concentrations of biomagnifying contaminants, such as polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and mercury (Hg). These species also face climate-change-related impacts which are leading to alterations in their diet and associated contaminant exposure. The metabolomic profile of marine mammal tissues and how it changes to environmental stressors is poorly understood. This study characterizes the profiles of 235 metabolites across plasma, liver, and inner and outer blubber in adult ringed seals and beluga whales and assesses how these profiles change as a consequence of contaminants and dietary changes. In both species, inner and outer blubber were characterized by a greater proportion of lipid classes, whereas the dominant metabolites in liver and plasma were amino acids, carbohydrates, biogenic amines and lysophosphatidylcholines. Several metabolite profiles in ringed seal plasma correlated with δ13C, while metabolite profiles in blubber were affected by hexabromobenzene in ringed seals and PBDEs and Hg in belugas. This study provides insight into inter-matrix similarities and differences across tissues and suggests that plasma and liver are more suitable for studying changes in diet, whereas liver and blubber are more suitable for studying the impacts of contaminants.
Wind Predictions in the Lower Stratosphere: State of the Art and Application of the COSMO Limited Area Model
Authors: Bucchignani Edoardo
Published: August 2022

In the last few decades there has been increasing interest in the commercial usage of the stratosphere, especially for Earth observation systems. Stratospheric platforms allow Earth monitoring at a regional scale with persistency toward a limited area. For this reason, accurate meteorological forecasts are needed in order to guarantee stationarity. The main aim of this work is to provide a review of wind prediction techniques in the stratosphere, achieved by the most popular global models, such as ECMWF IFS, NCEP GFS and ICON. Then, the capabilities of the COSMO limited area model to reproduce the wind speed in the stratosphere are evaluated considering a model configuration with very high resolution (about 1 km) over a domain located in Southern Italy, assuming the radio sounding data at Pratica di Mare airport as the reference. Vertical profiles were analyzed for selected days, highlighting good performances, though improvements can be achieved by adopting a fifth-order interpolation of the model data. Finally, monthly wind speed time series for selected heights were post-processed by means of fast Fourier transform, revealing the existence of main frequencies and the presence of a scaling regime and a power law of the form f−β over a broad range of time scales, in the Fourier space. The exponent spectral β is close to the exact 5/3 Kolmogorov value for all the datasets.
Enzyme Nanosheet-Based Electrochemical Aspartate Biosensor for Fish Point-of-Care Applications
Authors: Rajarathinam Thenmozhi , Thirumalai Dinakaran , Jayaraman Sivaguru , Kim Seonghye , Kwon Minho , Paik Hyun-jong , Kim Suhkmann , Kang Mijeong , Chang Seung-Cheol
Published: August 2022

Bacterial infections in marine fishes are linked to mass mortality issues; hence, rapid detection of an infection can contribute to achieving a faster diagnosis using point-of-care testing. There has been substantial interest in identifying diagnostic biomarkers that can be detected in major organs to predict bacterial infections. Aspartate was identified as an important biomarker for bacterial infection diagnosis in olive flounder (Paralichthys olivaceus) fish. To determine aspartate levels, an amperometric biosensor was designed based on bi-enzymes, namely, glutamate oxidase (GluOx) and aspartate transaminase (AST), which were physisorbed on copolymer reduced graphene oxide (P-rGO), referred to as enzyme nanosheets (GluOx-ASTENs). The GluOx-ASTENs were drop casted onto a Prussian blue electrodeposited screen-printed carbon electrode (PB/SPCE). The proposed biosensor was optimized by operating variables including the enzyme loading amount, coreactant (α-ketoglutarate) concentration, and pH. Under optimal conditions, the biosensor displayed the maximum current responses within 10 s at the low applied potential of −0.10 V vs. the internal Ag/AgCl reference. The biosensor exhibited a linear response from 1.0 to 2.0 mM of aspartate concentrations with a sensitivity of 0.8 µA mM−1 cm−2 and a lower detection limit of approximately 500 µM. Moreover, the biosensor possessed high reproducibility, good selectivity, and efficient storage stability.
Liquid Metal-Based Flexible and Wearable Sensor for Functional Human–Machine Interface
Authors: Tao Ye , Han Feiyang , Shi Changrui , Yang Ruizhe , Chen Yixing , Ren Yukun
Published: August 2022

Rigid sensors are a mature type of sensor, but their poor deformation and flexibility limit their application range. The appearance and development of flexible sensors provide an opportunity to solve this problem. In this paper, a resistive flexible sensor utilizes gallium−based liquid metal (eutectic gallium indium alloy, EGaIn) and poly(dimethylsiloxane) (PDMS) and is fabricated using an injecting thin−line patterning technique based on soft lithography. Combining the scalable fabrication process and unique wire−shaped liquid metal design enables sensitive multifunctional measurement under stretching and bending loads. Furthermore, the flexible sensor is combined with the glove to demonstrate the application of the wearable sensor glove in the detection of finger joint angle and gesture control, which offers the ability of integration and multifunctional sensing of all−soft wearable physical microsystems for human–machine interfaces. It shows its application potential in medical rehabilitation, intelligent control, and so on.
Metagenomics Reveal Microbial Effects of Lotus Root–Fish Co-Culture on Nitrogen Cycling in Aquaculture Pond Sediments
Authors: Yang Zhen , Yao Yanhong , Sun Meng , Li Gu , Zhu Jianqiang
Published: August 2022

Feed input leads to a large amount of nitrogen-containing sediment accumulating in the substrate in the pond culture process, threatening the safety of aquaculture production. Planting lotus roots (Nelumbo nucifera Gaertn.) in ponds can accelerate the removal of bottom nitrogen, while the role of nitrogen cycle-related microorganisms in the removal is still unclear. In this study, eight yellow catfish (Pelteobagrus fulvidraco) culture ponds with the same basic situation were divided into fishponds with planted lotus roots and ponds with only fish farming. Sediment samples were taken from the fishponds with planted lotus roots and the ponds with only fish farming before and after fish farming, marked as FPB, FPA, FOB, and FOA, respectively, and subjected to physicochemical and metagenomic sequencing analyses. The results show that the contents of NH4+, NO2−, TN, TP, and OM were significantly lower (p < 0.05) in FPA than in FOA. The abundance of metabolic pathways for inorganic nitrogen transformation and ammonia assimilation increased considerably after culture compared to the sediments before culture. A total of eight ammonia production pathways and two ammonia utilization pathways were annotated in the sediments of the experimental ponds, with a very high abundance of ammonia assimilation. Acinetobacter and Pseudomonas (34.67%, 18.02%) were the dominant bacteria in the pond sediments before culture, which changed to Thiobacillus (12.16%) after culture. The FPA had significantly higher relative abundances of Thiobacillus denitrificans and Sulfuricella denitrificans, and the FOA had significantly a higher abundance of Microcystis aeruginosa compared to other samples. The massive growth of Microcystis aeruginosa provided two new inorganic nitrogen metabolic pathways and one organic nitrogen metabolic pathway for FOA. The relative abundances of these three microorganisms were negatively correlated with NH4+ content (p < 0.01) and significantly positively correlated with AP, OM content, and pH value. Compared with ponds with only fish farming, lotus root–fish co-culture can significantly reduce the nitrogen content in sediment, increase the abundance of denitrifying bacteria, and inhibit algae growth. Still, it has little effect on the abundance of nitrogen cycle-related enzymes and genes. In summary, it is shown that, although lotus roots promote the growth of denitrifying microorganisms in the sediment, nitrogen removal relies mainly on nutrient uptake by lotus roots.
Effect of Water Activity on Conidia Germination in Aspergillus flavus
Authors: Jia Sifan , Li Chong , Wu Kuntan , Qi Desheng , Wang Shuai
Published: August 2022

In this study, we explored the mechanism underlying Aspergillus flavus conidia germination inhibited by decreased water activity. The impact of low water activity was analyzed at 4 h, 8 h and 12 h. Additionally, we demonstrated that low water activity affected cell shape and decreased cell sizes. Transcriptomics found numerous differentially expressed genes (DEGs) during the first 12 h of germination, with 654 DEGs observed among 4 h, 8 h and 12 h. In particular, more DEGs were detected at 8 h of germinating. Therefore, proteomics was performed at 8 h, and 209 differentially expressed proteins (DEPs) were speculated, with 94 up-regulated and 115 down-regulated. Combined analysis of KEGG of transcriptomics and proteomics demonstrated that the dominant pathways were nutrient metabolism and translation. We also found several DEGs and DEPs in the Mitogen Activated Protein Kinase (MAPK) pathway. Therefore, we concluded that low water activity inhibited conidia germination, causing unregular morphology. In addition, low water activity influenced expression of creA, TreB in carbohydrate metabolism, Clr4, RmtA in amino acid metabolism and RPL37, RPL3 in translation in Aspergillus flavus.
Geochronology and Geochemistry of the Xianghualing Granitic Rocks: Insights into Multi-Stage Sn-Polymetallic Mineralization in South China
Authors: Luo Zhaoyang , Li Huan , Wu Jinghua , Sun Wenbo , Zhou Jianqi , Maulana Adi
Published: August 2022

Multi-stage magmatic events associated with large tungsten-tin polymetallic deposits in the Nanling Range have been the subject of extensive research spanning many years. In this paper we report the results of a systematic study of the petrology, whole-rock geochemistry, zircon U-Pb chronology, and trace element geochemistry of granite bodies exposed in the Xianghualing ore field. They show that the granites in the study area are characterized by high SiO2 (63.83%–75.29%), Al2O3 (13.12%–18.87%), Rb (565–3260 ppm), Nd (67.3–113.5 ppm) and Ta (23.2–129.0 ppm) and by low MgO (0.02%–0.22%), TiO2 (0%–0.02%), Sr (5.3–80.5 ppm) and Ba (7.9–66.4 ppm). The rocks are highly differentiated A-type peraluminous granite, which originated in an extensional within-plate tectonic setting. Based on U-Pb dating and trace element analysis, the following multi-stage magma-hydrothermal events were identified: (1) Paleozoic (~347 Ma) and Triassic (~206 Ma) magmatic stages (initial enrichment epochs of ore-forming elements), (2) Jurassic (~161 Ma) magmatic-hydrothermal stage (mineralization epoch), and (3) Cretaceous hydrothermal overprinting stage (with peaks in the Early Cretaceous ~120 Ma and Late Cretaceous ~80 Ma). From an economic point of view, the Late Cretaceous appears to have great potential for tungsten-tin mineralization. Zircon trace element geochemistry indicates that the ore-forming fluids related to tin mineralization in the Cretaceous originated from the crust and underwent highly differentiated evolutionary processes under relatively reducing conditions. This paper emphasizes the Cretaceous tungsten-tin metallogenic events in the Nanling Range and provides an essential basis and new ideas for further tin-tungsten exploration.
Major Factors Controlling the Elemental Composition of Al-Kharrar Lagoon Bottom Sediments, Rabigh, Saudi Arabia
Authors: Ghandour Ibrahim M. , Mannaa Ammar , Alharbi Omar , Masoud Alaa A. , Aljahdali Mohammed H.
Published: August 2022

Bottom sediments of Al-Kharrar Lagoon, Rabigh area, Saudi Arabia were analyzed for mineralogical (26 samples) and major oxides, minor, and trace elements (46 samples). The prime objective is to document the controlling factors of the mineralogical and chemical composition of the lagoon bottom sediments. Hierarchical cluster (HCA) and the principal component (PCA) analyses are used to disclose the degree of similarities among elements to distinguish them into statistically significant groups. Results clarified the interplay of terrestrial sediment influx through the temporarily active Wadi Rabigh, hydrological regime and the autochthonous biogenic sedimentation, and to a lesser extent rare anthropogenic influence that impacted the lagoon sediments. The spatial distribution of minerals shows a southward increase in the siliciclastic-related minerals (quartz, clay minerals, k-feldspars, and plagioclase along with traces of amphiboles), whereas carbonate minerals (high Mg-calcite and aragonite) dominate the northern sector of the lagoon in areas far from the influence of detrital influx. The concentrations levels of oxides, minor, and trace elements display spatial variability. Three main distinctive elemental groups were delineated appraising the analysis of the elemental interrelationships and associated statistical analysis. The first group includes the positively correlated SiO2, TiO2, Al2O3, Fe2O3, MnO, MgO, K2O, Na2O, V, Cr, Ni, Zn, Rb, and Ba, which are concentrated in the southern sector of the lagoon. The second group is the carbonate-related elements (CaO and Sr) that dominate the northern sector. The distribution patterns of P2O5 and Cu varied highly across the lagoon. Enrichment factors revealed moderate levels of Cu in some sites supporting the anthropogenic source. The results showed the hospitable bottom ecological status of the lagoon despite local anthropogenic stressors such as an influx of flood water that contain a mixture of lithogenic and dissolved Cu from local farming.
40Ar/39Ar Geochronology, Geochemistry and Petrogenesis of the Volcanic Rocks in the Jiangling Basin, China
Authors: Wang Chunlian , Yan Kai , Yu Xiaocan , Wang Jiuyi , Liu Dianhe , Shen Lijian , Li Ruiqin , You Chao
Published: August 2022

In this study, 40Ar/39Ar geochronology and major and trace element data were presented for Paleogene basaltic rocks from the Jiangling Basin, China. The volcanic rocks erupted at ca. 53.19–60.78 Ma and belonged to the sub alkaline series. These basaltic rocks are generally characterized by enrichment in large-ion lithophile elements (LILEs) and light rare earth elements (LREEs) ((La/Yb)cn = 6.14–11.72) and lack of Eu anomalies (Eu/Eu* = 0.98–1.09), similar to ocean island basalts. The geochemical signatures of these rocks are similar to hotspot-related Paleogene volcanic rocks in the North China Block and late Cenozoic volcanic rocks in Southeast China. The Cenozoic lithospheric mantle, as well as the Mesozoic basalts that are beneath the northern Yangtze Blocks, might be inherited from the Mesozoic lithospheric mantle. The basaltic rocks from the Jiangling Basin in the northern Yangtze Block were generated from the partial melting of EMII (enrichedmantleII)-like lithospheric mantle due to the intracontinental extension.
Early Triassic Monzonite–Granite Series in Eastern Kazakhstan as a Reflection of Siberian Large Igneous Province Activity
Authors: Khromykh Sergey V. , Kotler Pavel D. , Kulikova Anna V. , Semenova Dina V. , Minnebaev Kamil R. , Gareev Bulat I. , Batalin Georgii A. , Antsiferova Tatiana N. , Il’icheva Ekaterina A. , Volosov Alexey S.
Published: August 2022

We provide the results of studying the internal structure and composition of the rocks of the Semeitau and Delbegetei massifs located in Eastern Kazakhstan. It was previously believed that these massifs have different ages and were formed in different geodynamic settings. The U-Pb zircon age from the monzonites and quartz monzonites was determined to be 249 ± 2 Ma, which showed the same Early Triassic age of the massifs. Both massifs are composed of rocks of the same monzonite–granite series of rocks with a significant proportion of high-silica rocks (leucocratic granites). Intermediate rocks are formed due to the differentiation of subalkaline mafic magmas, and the felsic rocks (rhyolites and granites) are the result of partial melting of crustal substrates. The massif formation model assumes the intrusion of mafic magmas into the crust, their differentiation and mixing with crustal melts, and then the intrusion of various rocks into the upper crustal levels. Analysis of the geological position, age and composition of the rocks allows us to conclude that the Semeitau and Delbegetei massifs were formed in an intraplate geodynamic setting. The activity of the mantle plume is the most probable reason for their formation. The Semeitau and Delbegetei massifs can be included in the southern part of the range of the Early Triassic Siberian Large Igneous Province.