bluuebunny/crossref_metadata_2025 huggingface dataset:
Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
Authors: Zambarbieri Jari , Moretti Pierangelo , Giordano Alessia , Scarpa Paola
Published: November 2021

The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoassays. The aim of this study was to perform the analytical validation of a new immunoenzymatic method for the measurement of feline PTH. Thirty-eight cats affected with chronic kidney disease (CKD) were included. PTH was measured using a two-site immunoenzymatic method validated in humans and dogs (ST AIA-PACK® Intact PTH, Tosoh Bioscience, Tessenderlo, Belgium). The analytical validation provided the evaluation of precision (intra-assay and inter-assay), accuracy (linearity under dilution (LUD) and spike recovery test (SRT)), and the storage stability of serum samples at 20 °C, 4 °C, and −20 °C. The method showed good precision (intra-assay CVs (coefficient of variations) 3.19–9.61%; inter-assay CVs 9.26–15.28%). In both the intra- and inter-assays, the highest imprecision was found with the low concentration pool (9.61% and 15.28%) and accuracy (LUD and SRT r2 = 0.99, p < 0.001), while the stability was optimal up until 7 days at −20 °C (−7.7%). The method was successfully validated in cats, allowing its future use in diagnostic procedures.
Daytime Grazing in Mountainous Areas Increases Unsaturated Fatty Acids and Decreases Cortisol in the Milk of Holstein Dairy Cows
Authors: Ghassemi Nejad Jalil , Lee Bae-Hun , Kim Ji-Yung , Sung Kyung-Il , Lee Hong-Gu
Published: November 2021

The effects of grazing lactating cows in mountainous areas for 12 and 24 h compared with the confined indoor system were evaluated by examining the overall milk fatty acid and cortisol. Twenty-one dairy cows were allocated to three treatment groups: (1) control (confined management system in a free-stall barn; TMR based), (2) grazing for 12 h (12 hG; TMR plus grazing pasture), and (3) grazing for 24 h (24 hG; pasture-based feeding system). Dry matter intake was higher in the control and 12 hG groups than in the 24 hG group. The yields of total milk and the 3.5% fat-corrected milk were the lowest in the 24 hG group. Milk fat was the highest in the 24 hG group and higher in 12 hG compared with the control group. Milk protein and lactose levels were the highest in the 12 hG group. The highest somatic cell count was observed in the 24 hG group. The saturated fatty acid levels were higher in the control group compared with the 12 hG and 24 hG groups. There was no difference in overall mono-unsaturated fatty acids between 12 hG and 24 hG groups. Poly-unsaturated fatty acids were higher in the 12 hG group compared with the control and 24 hG groups. There was no difference in omega-6 (ω-6) fatty acids among the groups, and omega-3 fatty acids were higher in the 12 hG group than in the control group. Milk cortisol was the highest in the 24 hG group and higher in the control group compared with the 12 hG group. Taken together, grazing for 12 h is advisable for farms that have access to mountainous areas to improve the milk fatty acid profile and decrease the stress levels in high-yielding Holstein lactating cows.
Transforming the Emotional Intelligence of the Feeders in Agribusinesses into the Development of Task Performance and Counterproductive Work Behaviors during the COVID-19 Pandemic
Authors: Huang Stanley Y. B. , Li Ming-Way , Lee Yue-Shi
Published: November 2021

The present research poses a novel multilevel model to describe how transformational leadership can significantly affect task performance and counterproductive work behavior through intermediary effects of emotional intelligence, work engagement, and work burnout. The empirical data is from 240 livestock feeders from 80 Taiwanese livestock production agribusinesses. The empirical results demonstrate that leadership could indeed transform the emotional intelligence of livestock feeders into positive task performance and negative counterproductive work behavior. The research results can provide an implementation method for livestock production agribusinesses to achieve the sustainable work of feeders in agribusinesses through handling task performance and counterproductive work behavior of feeders.
Association of Thoroughbred Racehorse Workloads and Rest Practices with Trainer Success
Authors: Morrice-West Ashleigh , Hitchens Peta , Walmsley Elizabeth , Wong Adelene , Whitton R.
Published: November 2021

Understanding the relationship between the training practices of Thoroughbred racehorses and race performance is important to ensure advice given to trainers for injury prevention or management is practical and consistent. We assessed associations between intended volume and speed of gallop training (i.e., typical workloads for horses free of injury or other performance limiting conditions) and rest practices on official trainer career and previous season success rates (rate of wins and places, prizemoney per start). Sixty-six Australian Thoroughbred trainers were surveyed. Multivariable negative binomial regression models were employed for the outcomes career and previous season wins and places, and linear regression models for prizemoney per start. Intended training workload was not associated with prizemoney. Pre-trial total galloping distances (≥13.3 m/s) between 7500 m and 15,000 m were associated with a higher rate of career wins, and previous season wins and places per start (p < 0.05). Slow-speed (13.3–14.3 m/s) galloping distance to trial between 5000 m to 12,500 m was associated with higher rate of career placings per start, with reduced performance over 12,500 m (p = 0.003). Greater time between race starts was associated with a greater rate of previous season wins and prizemoney per start until three weeks between starts, with decline in performance thereafter (p < 0.05). Greater frequency of rest breaks was associated with greater prizemoney per start earnt in the previous season (p ≤ 0.01). These results suggest that modifications to training programs aimed at injury prevention, such as avoiding long galloping distances, should not adversely affect trainer success.
Antifungal Activity of Essential Oils from Three Artemisia Species against Colletotrichum gloeosporioides of Mango
Authors: Huang Xing , Liu Tiantian , Zhou Chunxiang , Huang Yulin , Liu Xing , Yuan Haibin
Published: November 2021

Post-harvest diseases of mango reduce fruit quality and cause severe yield losses with completely unmarketable fruits. The most common diseases of mangos are anthracnose (Colletotrichum gloeosporioides). In this study, the antibacterial activities of essential oils from Artemisia scoparia, Artemisia lavandulaefolia, and Artemisia annua against C. gloeosporioides were tested. The results showed that the essential oil of A. scoparia was more effective by the agar diffusion method; the EC50 value was 9.32 µL/mL. The inhibition rate was 100%, at a concentration of 10 μL/mL, through the spore germination method. The morphological changes of the mycelium were observed by scanning electron microscopy (SEM), the mycelia treated with essential oils showed shrinking, deformity, fracture, and dryness through SEM. A. scoparia essential oil was inoculated in vivo and subjected to paroxysm testing under natural conditions. A. scoparia had significantly inhibitory activity, and the inhibition rate was 66.23% in vivo inoculation tests after 10 days. The inhibition rate was 92.06% in the paroxysm test under natural conditions after 15 days. Finally, A. acoparia essential oil was analyzed by gas chromatography-mass spectrometry. The main compounds were 2-ethenyl-Naphthalene (23.5%), 2,4-pentadiynyl-Benzene (11.8%), 1,2-dimethoxy-4-(2-propenyl)-Benzene (10.0%), β-Pinene (8.0%), and 1-methyl-4-(1-methylethyl)-1,4-Cyclohexadiene (6.3%). The results have revealed the potential use of A. scoparia essential oil against post-harvest fungal pathogens C. gloeosporioides.
Reliability Assessment of Space Station Based on Multi-Layer and Multi-Type Risks
Authors: Li Xiaopeng , Li Fuqiu
Published: November 2021

A space station is a typical phased-mission system, and assessing its reliability during its configuration is an important engineering action. Traditional methods usually require extensive data to carry out a layered reliability assessment from components to the system. These methods suffer from lack of sufficient test data, and the assessment process becomes very difficult, especially in the early stage of the configuration. This paper proposes a reliability assessment method for the space station configuration mission, using multi-layer and multi-type risks. Firstly, the risk layer and the risk type for the space station configuration are defined and identified. Then, the key configuration risks are identified comprehensively, considering their occurrence likelihood and consequence severity. High load risks are identified through risk propagation feature analysis. Finally, the configuration reliability model is built and the state probabilities are computed, based on the probabilistic risk propagation assessment (PRPA) method using the assessment probability data. Two issues are addressed in this paper: (1) how to build the configuration reliability model with three layers and four types of risks in the early stage of the configuration; (2) how to quantitatively assess the configuration mission reliability using data from the existing operational database and data describing the propagation features. The proposed method could be a useful tool for the complex aerospace system reliability assessment in the early stage.
Evaluation of Areal Monthly Average Precipitation Estimates from MERRA2 and ERA5 Reanalysis in a Colombian Caribbean Basin
Authors: Vega-Durán Jean , Escalante-Castro Brigitte , Canales Fausto A. , Acuña Guillermo J. , Kaźmierczak Bartosz
Published: November 2021

Global reanalysis dataset estimations of climate variables constitute an alternative for overcoming data scarcity associated with sparsely and unevenly distributed hydrometeorological networks often found in developing countries. However, reanalysis datasets require detailed validation to determine their accuracy and reliability. This paper evaluates the performance of MERRA2 and ERA5 regarding their monthly rainfall products, comparing their areal precipitation averages with estimates based on ground measurement records from 49 rain gauges managed by the Institute of Hydrology, Meteorology, and Environmental Studies (IDEAM) and the Thiessen polygons method in the Sinu River basin, Colombia. The performance metrics employed in this research are the correlation coefficient, the bias, the normalized root mean square error (NRMSE), and the Nash–Sutcliffe efficiency (NSE). The results show that ERA5 generally outperforms MERRA2 in the study area. However, both reanalyses consistently overestimate the monthly averages calculated from IDEAM records at all time and spatial scales. The negative NSE values indicate that historical monthly averages from IDEAM records are better predictors than both MERRA2 and ERA5 rainfall products.
Long-Term Patterns and Trends of Shortwave Global Irradiance over the Euro-Mediterranean Region
Authors: Galanaki Elissavet , Emmanouil George , Lagouvardos Konstantinos , Kotroni Vassiliki
Published: November 2021

The spatiotemporal patterns and trends of shortwave global irradiance (SWGI) are a crucial factor affecting not only the climate but also sectors of the economy. In this work, the ERA5-Land reanalysis dataset is employed and evaluated against in situ measurements from a dense network of surface stations operated by the National Observatory of Athens over Greece, revealing a good agreement between the two datasets. Then, the spatiotemporal variability of SWGI is investigated over the Euro-Mediterranean region (10° W–42° E and 30° N–52° N) for a 40-year period (1981–2020). SWGI exhibits a smooth latitudinal variability from north to south of −5.4 W/m2/degree on an annual scale, while it varies significantly on a seasonal basis and is almost four times lower in the winter than in the summer. The SWGI trend during the analyzed period was found to be positive and statistically significant at the 95% confidence level. Spring and summer are the periods where positive and the strongest rates of SWGI trends are evident, while in the winter and autumn, negative or neutral trends were found. The increasing SWGI trend shows a slowdown during the beginning of the 2000s in all seasons, except autumn. The SWGI trend decreases by about −0.06 W/m2/decade every 100 m of elevation increase.
Air Pollution in New Delhi during Late Winter: An Overview of a Group of Campaign Studies Focusing on Composition and Sources
Authors: Talukdar Shamitaksha , Tripathi Sachchida Nand , Lalchandani Vipul , Rupakheti Maheswar , Bhowmik Himadri Sekhar , Shukla Ashutosh K. , Murari Vishnu , Sahu Ravi , Jain Vaishali , Tripathi Nidhi , Dave Jay , Rastogi Neeraj , Sahu Lokesh
Published: November 2021

In recent times, a significant number of studies on the composition and sources of fine particulate matters and volatile organic compounds have been carried out over Delhi, either initiated by or in association with the researchers from the Indian Institute of Technology Kanpur (IIT Kanpur), in collaboration with researchers from within and outside India. All these studies utilized highly time-resolved, campaign-mode observations made with state-of-the-art instrumentation during the late winter months (mid-January to March) of 2018. Individually, each of these studies were rigorous in nature, containing explicit detailing about different types of ambient air pollutants in Delhi such as organic aerosols, inorganic elements, metals, carbonaceous aerosols, and volatile organic compounds. This study consolidates the extremely useful knowledge on source attribution of these air pollutants in the Delhi National Capital Region currently contained in these fragmented studies, which is vital to further enhancing our understanding of composition, characteristics, and sources of air pollutants over Delhi, as well as to designing appropriate mitigation measures tailored to local specifics.
Assessment of Natural Radioactivity, Heavy Metals and Particulate Matter in Air and Soil around a Coal-Fired Power Plant—An Integrated Approach
Authors: Dinis Maria de Lurdes , Fiúza António , Góis Joaquim , de Carvalho José Soeiro , Meira Castro Ana C.
Published: November 2021

A comprehensive study of the environmental radioactivity covered in a distance up to 20 km from a point source—two stacks of a coal-fired power plant. Airborne particulate matter was collected, and the element composition on the 30 cm soil profile was determined. The range of activity concentrations of 226Ra, 232Th and 40K from the studied areas varies from 8 Bq/kg to 41 Bq/kg, 5 to 72 Bq/kg and 62 to 795 Bq/kg, respectively. The activities values are increased by 44% for 226Ra, 37% for 40K, and 75% for 232Th in the prevailing wind direction. For some elements, the respective concentration in the soil is above the maximum permissible level for all types of soil use, particularly for the arsenic concentration. The deposition flux ranged from 0.36 to 5.70 (g m−2 per month) in the first sampling campaign and from 0.02 to 3.10 (g m−2 per month) for the second sampling campaign. Maps on the spatial distribution of gamma dose rates, radionuclides activity concentrations, deposition flux and trace metals in topsoil were developed for the study region. These maps are in accordance with higher values in specific locations in the vicinity of the coal-fired power plant, showing the influence of point sources, and for locations within 6 and 20 km from the stacks, particularly in the prevailing wind direction.
Land Surface Temperature Retrieval Using High-Resolution Vertical Profiles Simulated by WRF Model
Authors: Diaz Lucas Ribeiro , Santos Daniel Caetano , Käfer Pâmela Suélen , Rocha Nájila Souza da , Costa Savannah Tâmara Lemos da , Kaiser Eduardo Andre , Rolim Silvia Beatriz Alves
Published: November 2021

This work gives a first insight into the potential of the Weather Research and Forecasting (WRF) model to provide high-resolution vertical profiles for land surface temperature (LST) retrieval from thermal infrared (TIR) remote sensing. WRF numerical simulations were conducted to downscale NCEP Climate Forecast System Version 2 (CFSv2) reanalysis profiles, using two nested grids with horizontal resolutions of 12 km (G12) and 3 km (G03). We investigated the utility of these profiles for the atmospheric correction of TIR data and LST estimation, using the moderate resolution atmospheric transmission (MODTRAN) model and the Landsat 8 TIRS10 band. The accuracy evaluation was performed using 27 clear-sky cases over a radiosonde station in Southern Brazil. We included in the comparative analysis NASA’s Atmospheric Correction Parameter Calculator (ACPC) web-tool and profiles obtained directly from the NCEP CFSv2 reanalysis. The atmospheric parameters from ACPC, followed by those from CFSv2, were in better agreement with parameters calculated using in situ radiosondes. When applied into the radiative transfer equation (RTE) to retrieve LST, the best results (RMSE) were, in descending order: CFSv2 (0.55 K), ACPC (0.56 K), WRF G12 (0.79 K), and WRF G03 (0.82 K). Our findings suggest that there is no special need to increase the horizontal resolution of reanalysis profiles aiming at RTE-based LST retrieval. However, the WRF results were still satisfactory and promising, encouraging further assessments. We endorse the use of the well-known ACPC and recommend the NCEP CFSv2 profiles for TIR atmospheric correction and LST single-channel retrieval.
El Niño-Southern Oscillation and Indian Ocean Dipole Modes: Their Effects on South American Rainfall during Austral Spring
Authors: Kayano Mary T. , Cerón Wilmar L. , Andreoli Rita V. , Souza Rodrigo A. F. , Souza Itamara P. , Canchala Teresita
Published: November 2021

This paper examines the effects of the tropical Pacific Ocean (TPO) and Indian Ocean Dipole (IOD) modes in the interannual variations of austral spring rainfall over South America (SA). The TPO mode refers to the El Niño-Southern Oscillation (ENSO). The isolated effects between IOD and TPO were estimated, events were chosen from the residual TPO (R-TPO) or residual IOD (R-IOD), and the IOD (TPO) effects for the R-TPO (R-IOD) composites were removed from the variables. One relevant result was the nonlinear precipitation response to R-TPO and R-IOD. This feature was accentuated for the R-IOD composites. The positive R-IOD composite showed significant negative precipitation anomalies along equatorial SA east of 55° W and in subtropical western SA, and showed positive anomalies in northwestern SA and central Brazil. The negative R-IOD composite indicated significant positive precipitation anomalies in northwestern Amazon, central–eastern Brazil north of 20° S, and western subtropical SA, and negative anomalies were found in western SA south of 30° S. This nonlinearity was likely due to the distinct atmospheric circulation responses to the anomalous heating sources located in longitudinally distinct regions: the western tropical Indian Ocean and areas neighboring Indonesia. The results obtained in this study might be relevant for climate monitoring and modeling studies.
Size-Segregated Particulate Matter Down to PM0.1 and Carbon Content during the Rainy and Dry Seasons in Sumatra Island, Indonesia
Authors: Amin Muhammad , Putri Rahmi Mulia , Handika Rizki Andre , Ullah Aulia , Goembira Fadjar , Phairuang Worradorn , Ikemori Fumikazu , Hata Mitsuhiko , Tekasakul Perapong , Furuuchi Masami
Published: November 2021

Size-segregated particulate matter (PM) including the PM0.1 fraction, particles ≤0.1 µm, was monitored during the rainy and dry seasons at three different cities in Sumatra island, Indonesia in 2018. In order to identify possible emission sources, carbonaceous components in the particles collected by a cascade air sampler that is capable of collecting PM0.1 particles were analyzed by applying a thermal/optical reflectance (IMPROVE-TOR) protocol. The PM0.1 levels in the Jambi and Pekanbaru areas were similar to those in large cities in East Asia, such as Bangkok and Hanoi. During the rainy season, local emissions in the form of vehicle combustion were the main sources of PM. The influence of peatland fires in the dry season was more significant in cities that are located on the east coast of Sumatra island because of the larger number of hotspots and air mass trajectories along the coast. A clear increase in the carbonaceous profiles as OC, TC, and OC/EC ratios in the dry season from the rainy season was observed, particularly in fine fractions such as PM0.5–1. In both seasons, EC vs. OC/EC correlations and soot-EC/TC ratios showed that the PM0.1 fraction in Sumatra island was heavily influenced by vehicle emissions, while the effect of biomass burning was more sensitive with respect to the PM0.5–1 fraction, particularly in Jambi and Pekanbaru sites during the dry season.
Study of Atmospheric Pollution and Health Risk Assessment: A Case Study for the Sharjah and Ajman Emirates (UAE)
Authors: Nazzal Yousef , Orm Nadine Bou , Barbulescu Alina , Howari Fares , Sharma Manish , Badawi Alaa E. , A. Al-Taani Ahmed , Iqbal Jibran , Ktaibi Farid El , Xavier Cijo M. , Dumitriu Cristian Stefan
Published: November 2021

Dust is a significant pollution source in the United Arab Emirates (UAE) that impacts population health. Therefore, the present study aims to determine the concentration of heavy metals (Cd, Pb, Cr, Cu, Ni, and Zn) in the air in the Sharjah and Ajman emirates’ urban areas and assesses the health risk. Three indicators were used for this purpose: the average daily dose (ADD), the hazard quotient (HQ), and the health index (HI). Data were collected during the period April–August 2020. Moreover, the observation sites were clustered based on the pollutants’ concentration, given that the greater the heavy metal concentration is, the greater is the risk for the population health. The most abundant heavy metal found in the atmosphere was Zn, with a mean concentration of 160.30 mg/kg, the concentrations of other metals being in the following order: Ni > Cr > Cu > Pb > Cd. The mean concentrations of Cd, Pb, and Cr were within the range of background values, while those of Cu, Ni, and Zn were higher than the background values, indicating anthropogenic pollution. For adults, the mean ADD values of heavy metals decreased from Zn to Cd (Zn > Ni > Cr > Cu > Pb > Cd). The HQ (HI) suggested an acceptable (negligible) level of non-carcinogenic harmful health risk to residents’ health. The sites were grouped in three clusters, one of them containing a single location, where the highest concentrations of heavy metals were found.
Spatiotemporal Variability of Drought and Its Multi-Scale Linkages with Climate Indices in the Huaihe River Basin, Central China and East China
Authors: Fang Guohua , Li Xin , Xu Ming , Wen Xin , Huang Xianfeng
Published: November 2021

With the aggravation of the ocean–atmosphere cycle anomaly, understanding the potential teleconnections between climate indices and drought/flood conditions can help us know natural hazards more comprehensively to better cope with them. This study aims at exploring the spatiotemporal patterns of drought and its multi-scale relations with typical climate indices in the Huaihe River Basin. First, the spatial patterns were identified based on the seasonal Standardized Precipitation Index (SPI)-3 during 1956–2020 by means of the Empirical Orthogonal Function (EOF). The two leading sub-regions of spring and winter droughts were determined. Then, we extracted the periodicity of spring and winter SPI-3 series and the corresponding seasonal climate indices (Arctic Oscillation (AO), Bivariate El Niño–Southern Oscillation (ENSO)Timeseries (BEST), North Atlantic Oscillation (NAO), Niño3, and Southern Oscillation Index (SOI)) and the sunspot number by using the Continuous Wavelet Transform (CWT). We further explored the teleconnections between spring drought, winter drought, and climate indices and the sunspot number by using Cross Wavelet Transform (XWT) and Wavelet Coherence (WTC) analyses. The results show that there are in-phase multi-scale relations between spring/winter PC1 and AO, BEST, and Niño3, of which the climate indices lead spring PC1 by 1.5–2 years and the climate indices lag winter PC1 by 1.5–3 years. Anti-phase relations between spring PCs and SOI and the sunspot number were observed. NAO mainly affects the interdecadal variation in spring drought, while AO and Niño3 focus on the interannual variation. In addition, Niño3 and SOI are more related to the winter drought on interdecadal scales. Moreover, there is a positive correlation between the monthly average precipitation/temperature and Niño3 with a lag of 3 months. The results are beneficial for improving the accuracy of drought prediction, considering taking NAO, AO, and Niño3 as predictors for spring drought and Niño3 and SOI for winter drought. Hence, valuable information can be provided for the management of water resources as well as early drought warnings in the basin.
On Algebraic Independence of Solutions of Generalized Hypergeometric Equations
Authors: Gorelov Vasily
Published: November 2021

We present solutions for general theorems regarding algebraic independence of solutions of hypergeometric equation ensembles and the values of these solutions at algebraic points. The conditions of the theorems are necessary and sufficient. Furthermore, errors in theorems from F. Beukers and others are corrected.
Study of Wine Volatile Composition of Tempranillo versus Tempranillo Blanco, a New White Grape Variety
Authors: Garde-Cerdán Teresa , Rubio-Bretón Pilar , Marín-San Román Sandra , Baroja Elisa , Sáenz de Urturi Itziar , Pérez-Álvarez Eva P.
Published: November 2021

The aim of this work was to analyze and compare the concentration of higher alcohols, esters, and acids in wines from Tempranillo and Tempranillo Blanco. Tempranillo Blanco is a new and little-studied white variety that originated from Tempranillo by a natural mutation. During three seasons, grapevines of both varieties were harvested, and nine wines were made from each. The volatile composition of the wines was determined by GC-MS. In the wines of both varieties, the content of higher alcohols was higher than those of esters and acids. Wines from Tempranillo Blanco had lower content of 2-phenylethanol, methionol, 1-hexanol, benzyl alcohol, and total higher alcohols, but higher hexyl acetate and ethyl decanoate than Tempranillo wines. Total ethyl esters and total esters were higher in Tempranillo wines due to the higher ethyl lactate and ethyl succinate content derivate from the malolactic fermentation that was not made in Tempranillo Blanco. The content of hexanoic and octanoic acids and total acids was also higher in Tempranillo Blanco wines than in Tempranillo. This is one of the first studies carried out on the wine volatile composition of Tempranillo Blanco and therefore contributes to a better understanding of the oenological characteristics of this white variety.
Anethole Stability in Aniseed Spirits: Storage Condition Repercussions on Commercial Products
Authors: Vendramin Veronica , Pesce Antonio , Vincenzi Simone
Published: November 2021

Background: trans-anethole is the main component of anise, and its flavor is characteristic of all the aniseed spirits. It is known that trans-anethole oil/ethanol/water solution could result in the undesirable emulsion effect called the “ouzo effect”. Moreover, trans-anethole can undergo chemical decay, producing unhealthy compounds, mainly cis-anethole and para-anisaldehyde. In this work, the trans-anethole stability was challenged simulating commercial products’ long-term storage. Methods: Anethole standard solutions and two Italian aniseed spirits, namely Sambuca and Mistrà, were used to evaluate the trans-anethole response to light (both ultraviolet and visible) and its stability under thermal stress. Anethole decay was monitored by trans-anethole quantification using an HPLC method. Results: Light and high-temperature exposure affect the trans-anethole content of aniseed spirits during the storage, but sucrose revealed a role in the trans-anethole protection. Conclusions: as transport and storage are involved in the anethole decay, the Regulations on the minimum of the anethole that must be a guarantee in the aniseed spirits should be revised.
Composite Scaffolds from Gelatin and Bone Meal Powder for Tissue Engineering
Authors: Lantigua Darlin , Wu Xinchen , Suvarnapathaki Sanika , Nguyen Michelle A. , Camci-Unal Gulden
Published: November 2021

Bone tissue engineering offers versatile solutions to broaden clinical options for treating skeletal injuries. However, the variety of robust bone implants and substitutes remains largely uninvestigated. The advancements in hydrogel scaffolds composed of natural polymeric materials and osteoinductive microparticles have shown to be promising solutions in this field. In this study, gelatin methacrylate (GelMA) hydrogels containing bone meal powder (BP) particles were investigated for their osteoinductive capacity. As natural source of the bone mineral, we expect that BP improves the scaffold’s ability to induce mineralization. We characterized the physical properties of GelMA hydrogels containing various BP concentrations (0, 0.5, 5, and 50 mg/mL). The in vitro cellular studies revealed enhanced mechanical performance and the potential to promote the differentiation of pre-osteoblast cells. The in vivo studies demonstrated both promising biocompatibility and biodegradation properties. Overall, the biological and physical properties of this biomaterial is tunable based on BP concentration in GelMA scaffolds. The findings of this study offer a new composite scaffold for bone tissue engineering.
Bispecific T-Cell Engagers Targeting Membrane-Bound IgE
Authors: Rodak Aleksandra , Stadlmayr Gerhard , Stadlbauer Katharina , Lichtscheidl Dominic , Bobbili Madhusudhan Reddy , Rüker Florian , Wozniak-Knopp Gordana
Published: November 2021

The increased incidence of allergies and asthma has sparked interest in IgE, the central player in the allergic response. Interaction with its high-affinity receptor FcεRI leads to sensitization and allergen presentation, extracellular membrane-proximal domain in membrane IgE can act as an antigen receptor on B cells, and the interaction with low-affinity IgE receptor CD23 additionally influences its homeostatic range. Therapeutic anti-IgE antibodies act by the inhibition of IgE functions by interfering with its receptor binding or by the obliteration of IgE-B cells, causing a reduction of serum IgE levels. Fusion proteins of antibody fragments that can act as bispecific T-cell engagers have proven very potent in eliciting cytotoxic T-lymphocyte-mediated killing. We have tested five anti-IgE Fc antibodies, recognizing different epitopes on the membrane-expressed IgE, for the ability to elicit specific T-cell activation when expressed as single-chain Fv fragments fused with anti-CD3ε single-chain antibody. All candidates could specifically stain the cell line, expressing the membrane-bound IgE-Fc and bind to CD3-positive Jurkat cells, and the specific activation of engineered CD3-overexpressing Jurkat cells and non-stimulated CD8-positive cells was demonstrated for 8D6- and ligelizumab-based bispecific antibodies. Thus, such anti-IgE antibodies have the potential to be developed into agents that reduce the serum IgE concentration by lowering the numbers of IgE-secreting cells.
Endothelial Dysfunction: From Pathophysiology to Novel Therapeutic Approaches
Authors: Jeon Byeong Hwa
Published: November 2021

The vascular endothelium is an active tissue that plays a crucial role in the maintenance of vascular homeostasis [...]
Bone Marrow-Derived Mesenchymal Stem Cells Migrate toward Hormone-Insensitive Prostate Tumor Cells Expressing TGF-β via N-Cadherin
Authors: Noh Jinok , Yu Jinyeong , Kim Wootak , Park Aran , Park Ki-Sook
Published: November 2021

The prostate tumor microenvironment plays important roles in the metastasis and hormone-insensitive re-growth of tumor cells. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are recruited into prostate tumors to facilitate tumor microenvironment formation. However, the specific intrinsic molecules mediating BM-MSCs’ migration to prostate tumors are unknown. BM-MSCs’ migration toward a conditioned medium (CM) of hormone-insensitive (PC3 and DU145) or hormone-sensitive (LNCaP) prostate tumor cells was investigated using a three-dimensional cell migration assay and a transwell migration assay. PC3 and DU145 expressed transforming growth factor-β (TGF-β), but LNCaP did not. Regardless of TGF-β expression, BM-MSCs migrated toward the CM of PC3, DU145, or LNCaP. The CM of PC3 or DU145 expressing TGF-β increased the phosphorylation of Smad2/3 in BM-MSCs. Inactivation of TGF-β signaling in BM-MSCs using TGF-β type 1 receptor (TGFBR1) inhibitors, SB505124, or SB431542 did not allow BM-MSCs to migrate toward the CM. The CM of PC3 or DU145 enhanced N-cadherin expression on BM-MSCs, but the LNCaP CM did not. SB505124, SB431542, and TGFBR1 knockdown prevented an increase in N-cadherin expression. N-cadherin knockdown inhibited the collective migration of BM-MSCs toward the PC3 CM. We identified N-cadherin as a mediator of BM-MSCs’ migration toward hormone-insensitive prostate tumor cells expressing TGF-β and introduced a novel strategy for controlling and re-engineering the prostate tumor microenvironment.
Neuropeptides Involved in Facial Nerve Regeneration
Authors: Kim Inhyeok , Kim Yonjae , Kang Daewoong , Jung Junyang , Kim Sungsoo , Rim Hwasung , Kim Sanghoon , Yeo Seung-Geun
Published: November 2021

Neuropeptides and neurotransmitters act as intermediaries to transmit impulses from one neuron to another via a synapse. These neuropeptides are also related to nerve degeneration and regeneration during nerve damage. Although there are various neuropeptides, three are associated with neural regeneration in facial nerve damage: calcitonin gene-related peptide (CGRP), galanin, and pituitary adenylyl cyclase-activating peptide (PACAP). Alpha CGRP in facial motoneurons is a signaling factor involved in neuroglial and neuromuscular interactions during regeneration. Thus, it may be a marker for facial nerve regeneration. Galanin is a marker of injured axons rather than nerve regeneration. PACAP has various effects on nerve regeneration by regulating the surrounding cells and providing neurotrophic factors. Thus, it may also be used as a marker for facial nerve regeneration. However, the precise roles of these substances in nerve generation are not yet fully understood. Animal studies have demonstrated that they may act as neuromodulators to promote neurotrophic factors involved in nerve regeneration as they appear early, before changes in the injured cells and their environment. Therefore, they may be markers of nerve regeneration.
Near Infrared Fluorescent Nanostructure Design for Organic/Inorganic Hybrid System
Authors: Okubo Kyohei , Umezawa Masakazu , Soga Kohei
Published: November 2021

Near infrared (NIR) light offers high transparency in biological tissue. Recent advances in NIR fluorophores including organic dyes and lanthanide-doped inorganic nanoparticles have realized the effective use of the NIR optical window for in vivo bioimaging and photodynamic therapy. The narrow energy level intervals used for electronic transition that involves NIR light, however, give rise to a need for guidelines for reducing heat emission in luminescence systems, especially in the development of organic/inorganic hybrid structures. This review presents an approach for employing the polarity and vibrational energy of ions and molecules that surround the luminescence centers for the development of such hybrid nanostructures. Multiphonon relaxation theory, formulated for dealing with heat release in ionic solids, is applied to describe the vibrational energy in organic or molecular systems, referred to as phonon in this review, and we conclude that surrounding the luminescence centers either with ions with low vibrational energy or molecules with small chemical polarity is the key to bright luminescence. NIR photoexcited phosphors and nanostructures in organic/inorganic mixed systems, designed based on the guidelines, for photodynamic therapy are reviewed.
Motor Preparation and Execution for Performance Difficulty: Centroparietal Beta Activation during the Effort Expenditure for Rewards Task as a Function of Motivation
Authors: Wilhelm Ricardo A. , Threadgill A. Hunter , Gable Philip A.
Published: November 2021

Debate exists as to the effects of anxiety in performance-based studies. However, no studies have examined the influence of motivation both in preparation of a motor movement and during movement performance. The present study measured beta activation in preparation for and during execution of the effort expenditure for rewards task (EEfRT), a button-pressing task consisting of easy and hard trials. Results indicated that motor preparation (i.e., reduced beta activation) was greater in preparation for hard trials than for easy trials. Additionally, motor preparation decreased (i.e., beta activation increased) over the course of hard trial execution. These results suggest that motor preparation is enhanced prior to more challenging tasks but that motor preparation declines as participants become closer to completing their goal in each challenging trial. These results provide insight into how beta activation facilitates effort expenditure for motor tasks varying in difficulty and motivation. The impact of these results on models of anxiety and performance is discussed.
Analysis of Selected Risk Factors Depending on the Type of Cerebral Palsy
Authors: Sadowska Małgorzata , Sarecka-Hujar Beata , Kopyta Ilona
Published: November 2021

Background: Cerebral palsy (CP) is not a defined, separate disease classification, but a set of etiologically diverse symptoms that change with the child’s age. According to the up-to-date definition, CP is a group of permanent but not unchanging disorders of movement and/or posture and motor function, which are due to a nonprogressive interference, lesion, or abnormality of the developing/immature brain. CP is one of the most frequent causes of motor disability in children. The aim of the present study was to analyze whether selected risk factors may vary depending on particular types of CP. Methods: 181 children with CP (aged 4–17 years), hospitalized at the Department of Pediatrics and Developmental Age Neurology in Katowice in the years 2008–2016 were retrospectively analyzed in the present study. The assumed risk factors of CP were divided into two groups: 1—pre-conception and prenatal (mother’s age, family history of epilepsy, burdened obstetric history, mother’s systemic diseases, pregnancy order, multiple pregnancy, duration of pregnancy, bleedings from the genital tract during gestation, arterial hypertension during pregnancy, infections during pregnancy, preterm contractions, maintained pregnancy, premature rupture of membranes, abruptio placentae, and others), 2—perinatal and postnatal (mode of delivery, birth weight, Apgar score at the first and fifth minute, neonatal convulsions, respiratory failure, infections in neonatal and infant period, and intraventricular bleeding). The division into particular CP types was based on Ingram’s classification. Results: The following risk factors were the most frequent in the total group: respiratory failure, infections, intraventricular bleeding, and prematurity. Among the analyzed preconception and prenatal factors, the duration of pregnancy and preterm contractions during pregnancy significantly differentiated the subgroups of patients depending on the type of CP. The prevalence of almost all analyzed perinatal, neonatal, and infant-related risk factors (i.e., birth weight, Apgar score at the first and fifth minute, neonatal convulsions, respiratory failure, infections in neonatal and infant period, and intraventricular bleeding) significantly differed between CP types, apart from the mode of delivery. However, in multivariate regression, only intraventricular bleeding was an independent predictor for tetraplegic CP type when compared to joined extrapyramidal and ataxic types (OR = 2.801, p = 0.028). Conclusions: As CP is a syndrome of multifactorial etiology, the identification of CP risk factors entails the need for careful observation and comprehensive care of children in the risk group. The presence of certain risk factors may be a prognostic indicator for particular types of CP. The knowledge about the association between the risk factor(s) and the CP type could be a very useful tool for pediatricians looking after the child at risk of developmental disorders.
Capturing Neuroplastic Changes after iTBS in Patients with Post-Stroke Aphasia: A Pilot fMRI Study
Authors: Xu Shuo , Yang Qing , Chen Mengye , Deng Panmo , Zhuang Ren , Sun Zengchun , Li Chong , Yan Zhijie , Zhang Yongli , Jia Jie
Published: November 2021

Intermittent theta-burst stimulation (iTBS) is a high-efficiency transcranial magnetic stimulation (TMS) paradigm that has been applied to post-stroke aphasia (PSA). However, its efficacy mechanisms have not been clarified. This study aimed to explore the immediate effects of iTBS of the primary motor cortex (M1) of the affected hemisphere, on the functional activities and connectivity of the brains of PSA patients. A total of 16 patients with aphasia after stroke received iTBS with 800 pulses for 300 s. All patients underwent motor, language, and cognitive assessments and resting-state functional MRI scans immediately before and after the iTBS intervention. Regional, seed-based connectivity, and graph-based measures were used to test the immediate functional effects of the iTBS intervention, including the fractional amplitude of low-frequency fluctuation (fALFF), degree centrality (DC), and functional connectivity (FC) of the left M1 area throughout the whole brain. The results showed that after one session of iTBS intervention, the fALFF, DC, and FC values changed significantly in the patients’ brains. Specifically, the DC values were significantly higher in the right middle frontal gyrus and parts of the left parietal lobe (p < 0.05), while fALFF values were significantly lower in the right medial frontal lobe and parts of the left intracalcarine cortex (p < 0.05), and the strength of the functional connectivity between the left M1 area and the left superior frontal gyrus was reduced (p < 0.05). Our findings provided preliminary evidences that the iTBS on the ipsilesional M1 could induce neural activity and functional connectivity changes in the motor, language, and other brain regions in patients with PSA, which may promote neuroplasticity and functional recovery.
Comparative Efficacy of Levetiracetam for Epilepsy in School-Aged Children with Intellectual Disability and Normal Intelligence
Authors: Moon Ja Un , Han Ji Yoon
Published: November 2021

Choosing optimal anti-seizure medication (ASM) is very important in pediatric patients with epilepsy who attend school, especially children with an intellectual disability (ID). Levetiracetam (LEV) has proven to be an effective, safe, generally well-tolerated, broad-spectrum ASM in children. In the context of increasing use of LEV in school-aged children with epilepsy and ID, we evaluate relevant clinical data, including efficacy, safety, and tolerability in children with epilepsy and an intellectual disability (ID) or normal intelligence (NI). We performed a retrospective chart review of children and included 298 pediatric patients with epilepsy who were treated with LEV with NI (147) and ID (151). After 6 months, 96% of NI and 83% of ID subjects had a seizure reduction rate greater than 50% (p = 0.031). The tolerability of LEV was generally good, with 75% retention rates at 2 years in both groups and only minor side effects (under 15%). The retention rates of patients with NI and ID were 76% and 74%, respectively (p = 0.597). Thus, LEV showed considerable efficacy with minimal side effects and high retention rates and is an easily maintained and safe treatment option for pediatric epilepsy with ID. However, better-designed research studies are needed to clearly elucidate the efficacy and safety of LEV in children with epilepsy and ID.
In Search of Digital Dopamine: How Apps Can Motivate Depressed Patients, a Review and Conceptual Analysis
Authors: Mouchabac Stephane , Maatoug Redwan , Conejero Ismael , Adrien Vladimir , Bonnot Olivier , Millet Bruno , Ferreri Florian , Bourla Alexis
Published: November 2021

Introduction: Depression is highly prevalent and causes considerable suffering and disease burden despite the existence of wide-ranging treatment options. Momentary assessment is a promising tool in the management of psychiatric disorders, and particularly depression. It allows for a real-time evaluation of symptoms and an earlier detection of relapse or treatment efficacy. Treating the motivational and hedonic aspects of depression is a key target reported in the literature, but it is time-consuming in terms of human resources. Digital Applications offer a major opportunity to indirectly regulate impaired motivational circuits through dopaminergic pathways. Objective: The main objective of this review was twofold: (1) propose a conceptual and critical review of the literature regarding the theoretical and technical principles of digital applications focused on motivation in depression, activating dopamine, and (2) suggest recommendations on the relevance of using these tools and their potential place in the treatment of depression. Material and Methods: A search for words related to “dopamine”, “depression”, “smartphone apps”, “digital phenotype” has been conducted on PubMed. Results: Ecological momentary interventions (EMIs) differ from traditional treatments by providing relevant, useful intervention strategies in the context of people’s daily lives. EMIs triggered by ecological momentary assessment (EMA) are called “Smart-EMI”. Smart-EMIs can mimic the “dopamine reward system” if the intervention is tailored for motivation or hedonic enhancement, and it has been shown that a simple reward (such as a digital badge) can increase motivation. Discussion: The various studies presented support the potential interest of digital health in effectively motivating depressed patients to adopt therapeutic activation behaviors. Finding effective ways to integrate EMIs with human-provided therapeutic support may ultimately yield the most efficient and effective intervention method. This approach could be a helpful tool to increase adherence and motivation. Conclusion: Smartphone apps can motivate depressed patients by enhancing dopamine, offering the opportunity to enhance motivation and behavioral changes, although longer term studies are still needed.