bluuebunny/crossref_metadata_2025 huggingface dataset:
Cellular Fitness Phenotypes of Cancer Target Genes from Oncobiology to Cancer Therapeutics
Authors: George Bijesh , Pillai P. Mukundan , Paul Aswathy Mary , Amjesh Revikumar , Leitzel Kim , Ali Suhail M. , Sandiford Oleta , Lipton Allan , Rameshwar Pranela , Hortobagyi Gabriel N. , Pillai Madhavan Radhakrishna , Kumar Rakesh
Published: February 2021

To define the growing significance of cellular targets and/or effectors of cancer drugs, we examined the fitness dependency of cellular targets and effectors of cancer drug targets across human cancer cells from 19 cancer types. We observed that the deletion of 35 out of 47 cellular effectors and/or targets of oncology drugs did not result in the expected loss of cell fitness in appropriate cancer types for which drugs targeting or utilizing these molecules for their actions were approved. Additionally, our analysis recognized 43 cellular molecules as fitness genes in several cancer types in which these drugs were not approved, and thus, providing clues for repurposing certain approved oncology drugs in such cancer types. For example, we found a widespread upregulation and fitness dependency of several components of the mevalonate and purine biosynthesis pathways (currently targeted by bisphosphonates, statins, and pemetrexed in certain cancers) and an association between the overexpression of these molecules and reduction in the overall survival duration of patients with breast and other hard-to-treat cancers, for which such drugs are not approved. In brief, the present analysis raised cautions about off-target and undesirable effects of certain oncology drugs in a subset of cancers where the intended cellular effectors of drug might not be good fitness genes and that this study offers a potential rationale for repurposing certain approved oncology drugs for targeted therapeutics in additional cancer types.
New Inhibitory Effects of Cilnidipine on Microglial P2X7 Receptors and IL-1β Release: An Involvement in its Alleviating Effect on Neuropathic Pain
Authors: Yamashita Tomohiro , Kamikaseda Sawako , Tanaka Aya , Tozaki-Saitoh Hidetoshi , Caaveiro Jose M. M. , Inoue Kazuhide , Tsuda Makoto
Published: February 2021

P2X7 receptors (P2X7Rs) belong to a family of ATP-gated non-selective cation channels. Microglia represent a major cell type expressing P2X7Rs. The activation of microglial P2X7Rs causes the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β). This response has been implicated in neuroinflammatory states in the central nervous system and in various diseases, including neuropathic pain. Thus, P2X7R may represent a potential therapeutic target. In the present study, we screened a chemical library of clinically approved drugs (1979 compounds) by high-throughput screening and showed that the Ca2+ channel blocker cilnidipine has an inhibitory effect on rodent and human P2X7R. In primary cultured rat microglial cells, cilnidipine inhibited P2X7R-mediated Ca2+ responses and IL-1β release. Moreover, in a rat model of neuropathic pain, the intrathecal administration of cilnidipine produced a reversal of nerve injury-induced mechanical hypersensitivity, a cardinal symptom of neuropathic pain. These results point to a new inhibitory effect of cilnidipine on microglial P2X7R-mediated inflammatory responses and neuropathic pain, proposing its therapeutic potential.
Effect of Fluid Flow on the Corrosion Performance of as-Cast and Heat-Treated Nickel Aluminum Bronze Alloy (UNS C95800) in Saline Solution
Authors: Jafari Hamid Reza , Davoodi Ali , Hosseinpour Saman
Published: February 2021

In this work, the corrosion behavior and surface reactivity of as-cast and heat-treated nickel aluminum bronze casting alloy (UNS C95800) in 3.5 wt% NaCl solution is investigated under stagnant and flow conditions. Increasing flow rate conditions are simulated using a rotating disk electrode from 0 to 9000 revolutions per minute (rpm). Optical micrographs confirm the decrease in the phase fraction of corrosion-sensitive β phase in the microstructure of C95800 after annealing, which, in turn, enhances the corrosion resistance of the alloy. Electrochemical studies including open circuit potentiometry, potentiodynamic polarization, and electrochemical impedance spectroscopy are performed to assess the effect of flow rate and heat treatment on the corrosion of samples at 25 and 40 °C in 3.5 wt% NaCl solution. For both as-cast and heat-treated samples, increasing the flow rate (i.e., electrode rotating rate) linearly reduces the corrosion resistance, indicating that the metal dissolution rate is significantly affected by hydrodynamic flow. Increasing the solution temperature negatively impacts the corrosion behavior of the as-cast and heat-treated samples at all flow conditions.
Influence of Phosphoric Acid on the Adhesion Strength between Rusted Steel and Epoxy Coating
Authors: Li Yang , Lei Bing , Guo Xuqiang
Published: February 2021

In this work, we evaluated the influence of phosphoric acid in conjunction with a tannic converter formula on the adhesion of the coating system by employing the epoxy coating as the top coating. The morphology and the composition of the rusted steel before and after treatment were determined by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The adhesion test result showed that the adhesion strength between the rust and the epoxy coating was enhanced from 1.93 MPa to 11.63 MPa with the treatment by 50 g/L phosphoric acid in conjunction with a tannic converter formula. Further investigation of the working mechanism of such treatment revealed that the adhesion improvement could be attributed to the formation of a micro-cracked and compact conversion layer. This layer could reinforce the anchoring of epoxy coating through the pinning effect. It was also found that both the pH and the phosphate radical were the important factors to improve the adhesion strength.
Contribution of Different Pretreatments to the Thermal Stability and UV Resistance Performance of Cellulose Nanofiber Films
Authors: Luo Lianxin , Wang Xuchong , Zhang Sheng , Yuan Xiaojun , Li Mingfu , Wang Shuangfei
Published: February 2021

Hot water (HW), green liquor (GL), and sodium chlorite (SC) pretreatments were used to pretreat sugarcane bagasse (SCB) and spruce (SP) and then to prepare cellulose nanofibers (CNFs) through high-pressure homogenization to explore the effect of physicochemical properties on the thermal stability and ultraviolet (UV) resistance performance of CNF films. The results indicated that the lignin content of HW-pretreated CNFs was higher than that of GL- and SC-pretreated CNFs, and the hemicellulose content of HW-pretreated CNFs was lower than that of GL- and SC-pretreated CNFs. The synergy of lignin and hemicellulose impacted the thermal stability of CNF films. The thermal stability of all the SP CNF films was higher than that of all the SCB CNF films. Hot water pretreatment improved the thermal stability of CNF films, and green liquor and sodium chlorite pretreatment decreased the thermal stability of CNF films. The highest thermal stability of SP-HW CNF films reached 392 °C, which was 5.4% higher than that of SP-SC CNF films. Furthermore, the ultraviolet resistance properties of different CNF films were as follows: SCB-HW > SCB-GL > SCB-SC and SP-HW > SP-GL > SP-SC. Green liquor pretreatment is an effective method to prepare CNFs. Conclusively, this research provides a basic theory for the preparation of CNFs and allows the improvement of CNF films in the application of thermal stability management and UV resistance fields.
D-karyo—A New Prenatal Rapid Screening Test Detecting Submicroscopic CNVs and Mosaicism
Authors: Shimokawa Osamu , Takeda Masayoshi , Ohashi Hiroyasu , Shono-Ota Akemi , Kumagai Mami , Matsushika Risa , Masuda Chika , Uenishi Kohtaro , Kimata Pooh Ritsuko
Published: February 2021

Chromosomal microarray analysis (CMA), recently introduced following conventional cytogenetic technology, can detect submicroscopic copy-number variations (CNVs) in cases previously diagnosed as “cytogenetically benign”. At present, rapid and accurate chromosomal analysis is required in prenatal diagnostics, but prenatal CMA is not widely used due to its high price and long turnaround time. We introduced a new prenatal screening method named digital karyotyping (D-karyo), which utilizes a preimplantation genetic test for the aneuploidy (PGT-A) platform. First, we conducted a preliminary experiment to compare the original PGT-A method to our modified method. Based on the preliminary results, we decided to implement the modified strategy without whole-genome amplification (WGA) and combined it with three analytical software packages. Next, we conducted a prospective study with 824 samples. According to the indication for invasive tests, the D-karyo positive rates were 2.5% and 5.0%, respectively, in the screening positive group with NT ≥ 3.5 mm and the group with fetal abnormalities by ultrasound. D-karyo is a breakthrough modality that can detect submicroscopic CNVs ≥ 1.0 Mb accurately in only 10.5 h for 24 samples at a low cost. Implementing D-karyo as a prenatal rapid screening test will reduce unnecessary CMA and achieve more accurate prenatal genetic testing than G-banding.
Towards Accurate Microwave Characterization of Tissues: Sensing Depth Analysis of Open-Ended Coaxial Probes with Ex Vivo Rat Breast and Skin Tissues
Authors: Aydinalp Cemanur , Joof Sulayman , Yilmaz Tuba
Published: February 2021

Dielectric properties of biological materials are commonly characterized with open-ended coaxial probes due to the broadband and non-destructive measurement capabilities. Recently, potential diagnostics applications of the technique have been investigated. Although the technique can successfully classify the tissues with different dielectric properties, the classification accuracy can be improved for tissues with similar dielectric properties. Increase in classification accuracy can be achieved by addressing the error sources. One well-known error source contributing to low measurement accuracy is tissue heterogeneity. To mitigate this error source, there is a need define the probe sensing depth. Such knowledge can enable application-specific probe selection or design. The sensing depth can also be used as an input to the classification algorithms which can potentially improve the tissue classification accuracy. Towards this goal, this work investigates the sensing depth of a commercially available 2.2 mm aperture diameter probe with double-layered configurations using ex vivo rat breast and skin tissues. It was concluded that the dielectric property contrast between the heterogeneous tissue components has an effect on the sensing depth. Also, a membrane layer (between 0.4–0.8 mm thickness) on the rat wet skin tissue and breast tissue will potentially affect the dielectric property measurement results by 52% to 84%.
Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study
Authors: Chong Yosep , Kim Tae Eun , Cho Uiju , Jin Min-Sun , Yim Kwangil , Thakur Nishant , Kim Jong Ok , Cho Inju , Park Gyeongsin
Published: February 2021

Diffuse large B-cell lymphoma (DLBCL) is the most common high-grade B-cell lymphoma found in Korea; it manifests with a variety of cellular morphologies and a high proliferation index. It is difficult to differentiate between DLBCL and Burkitt lymphoma (BL) based on immunohistochemistry, histology, and Epstein-Barr virus infection status owing to the overlap in findings. In this study, we performed comparative morphometric analysis to understand the proportional difference in Ki-67 staining between DLBCL and BL. We analyzed Ki-67-stained slides of 103 DLBCLs and 29 BLs that were pathologically confirmed using a three-tier classification system (negative, 1+, 2+, and 3+) to compare Ki-67 expression between BL and activated B-cell and germinal center B-cell subtypes of DLBCL and DLBCL with high proliferation indices (>90% of 2+ and 3+ cells). Patients with DLBCL were older than those with BL (62.1 versus 51.0 years). The number and proportion of negative cells (passenger and true negative cells) were significantly lower in BLs than those in DLBCLs (337.4, 5.9% versus 690.3, 12.4%). The number and proportion of 3+ cells were significantly higher in BLs than those in DLBCLs (5213.6, 96.3% versus 3132.4, 62.0%). BLs and DLBCLs with a high proliferation index showed similar results as those between BLs and overall DLBCLs. We were able to differentiate BLs and DLBCLs with 98.1% sensitivity and 100.0% specificity using an optimal cut-off of 97.9% of 2+/3+ Ki-67-positive cells. Thus, the Ki-67 labeling index may be a good differential biomarker for DLBCLs and BLs.
A Source of Systematic Errors in the Determination of Critical Micelle Concentration and Micellization Enthalpy by Graphical Methods in Isothermal Titration Calorimetry
Authors: Corea Mónica , Jiménez-Juárez Rogelio , Martínez-Mejía Gabriela , Martínez-Ortiz María , del Río José
Published: February 2021

Isothermal titration calorimetry is frequently employed to determine the critical micelle concentration and the micellization enthalpy of surfactants in terms of geometrical characteristics of the titration curves. Previously we have shown theoretically that even for an infinitesimal injection, the heat per titrant mol depends on the stock solution concentration. In this work, we explore experimentally the influence of the stock solution concentration on the geometrical characteristics of the titration curve and its effect in determining the critical micelle concentration and the micellization enthalpy of surfactants. The systematic study of this phenomenology involves a great number of measurements at different temperatures with several repetitions carried out using a robotic calorimeter. As surfactant hexadecyltrimethylamonium bromide was used. The magnitude and shape of the heat titration depend on the stock solution concentration. As a consequence, the inflexion-point, break-point, and step-height decrease until a limiting value. A qualitative analysis suggests that the limiting value depends only on substance. This work shows that graphical methods could not be suitable for the calculation of the critical micelle concentration and micellization enthalpy because the magnitude and shape of the titration curve depend on the stock solution concentration. Micellar properties should be calculated by the application of theoretical models as in the ligand-binding studies.
Threshold Computation for Spatially Coupled Turbo-Like Codes on the AWGN Channel
Authors: Farooq Muhammad Umar , Graell i Amat Alexandre , Lentmaier Michael
Published: February 2021

In this paper, we perform a belief propagation (BP) decoding threshold analysis of spatially coupled (SC) turbo-like codes (TCs) (SC-TCs) on the additive white Gaussian noise (AWGN) channel. We review Monte-Carlo density evolution (MC-DE) and efficient prediction methods, which determine the BP thresholds of SC-TCs over the AWGN channel. We demonstrate that instead of performing time-consuming MC-DE computations, the BP threshold of SC-TCs over the AWGN channel can be predicted very efficiently from their binary erasure channel (BEC) thresholds. From threshold results, we conjecture that the similarity of MC-DE and predicted thresholds is related to the threshold saturation capability as well as capacity-approaching maximum a posteriori (MAP) performance of an SC-TC ensemble.
Investigation into Sizing Photovoltaic with Energy Storage for Off-Grid Transactive Scenarios
Authors: Vance David , Razban Ali , Schubert Peter , Weissbach Robert
Published: February 2021

In this study, a novel sizing methodology was developed for centralized and interconnected operating strategies of transactive microgrids and several variables were investigated including starting month, initial charge of battery, load variability, unit cost of solar panels and energy storage, number of systems, climate, and required reliability to determine their effect on total cost. The centralized strategy improved cost by seven to ten percent compared to the isolated strategy in every case. The interconnected strategy saved an incremental amount of money consistently compared to the isolated standard. The number of connected systems was not a strong effect. It was thought that increasing the number of systems would increase the benefit of energy sharing. Climate zones studied (“Cold”; “Hot-Dry/Mixed Dry”; “Mixed Humid”; and “Cold but with lower solar irradiation”) showed a large variation on cost with the Hot-Dry/Mixed Dry being the least expensive and Cold, with lower solar irradiation being the most expensive. Cost sensitivity analysis was performed showing that the unit cost of solar has a greater effect on the total cost. Required reliability of power, measured in outage hours, exhibited an inverse relation with cost.
Accurate Online Battery Impedance Measurement Method with Low Output Voltage Ripples on Power Converters
Authors: Yao Qi , Lu Dylan-Dah-Chuan , Lei Gang
Published: February 2021

The conventional online battery impedance measurement method works by perturbing the duty cycle of the DC-DC power converter and measuring the response of the battery voltage and current. This periodical duty cycle perturbation will continuously generate large voltage ripples at the output of power converters. These large ripples will not easily be removed due to the high amplitude and wide frequency range and would be a challenge to meet tight output regulation. To solve this problem, this paper presents a new online battery impedance measurement technique by inserting a small switched resistor circuit (SRC) into the converter. The first contribution of this work is that the perturbation source is moved from the main switch to the input-side of the converter, so the ripples are reduced. The analysis and experimental results of the proposed method show a reduction of 16-times compared with the conventional method. The second contribution tackles the possible change of the battery state of charge (SOC) during the online battery measurement process, which will inevitably influence the impedance measurement accuracy. In this proposed method, battery impedance at multiple frequencies can be measured simultaneously using only one perturbation to accelerate measurement speed and minimize possible SOC change. The experimental impedance results coincide with a high-accuracy laboratory battery impedance analyzer.
Energy-Water-Carbon Nexus Optimization for the Path of Achieving Carbon Emission Peak in China Considering Multiple Uncertainties: A Case Study in Inner Mongolia
Authors: Liu Yuan , Tan Qinliang , Han Jian , Guo Mingxin
Published: February 2021

The Chinese government has launched a guideline for carbon emissions up to the peak (CEUP) in the 2030 target. The electric power sector has to make its own contributions to the national CO2 emissions mitigation target. In this study, a patron–client interactive optimized (PCIO) model is proposed to investigate the regional energy–water–carbon nexus optimization under the policy background of the CEUP target. Inner Mongolia, the largest energy base in China, which is also facing the prominent contradiction including the energy production and serious environmental problems, is chosen as a case study. Multiple uncertainties, including the fuel price uncertainty and output of the wind and solar power, are considered to make the optimization process more realistic. Results show that coal-fired power will gradually be substituted by the gas, wind, and solar power in Inner Mongolia to reach the CEUP target. The CO2 capture and storage technology and air-cooling systems will play important roles, especially under the strict water policy scenario. However, the achievement of the CEUP and water-saving target will be at the expense of high system costs. The PCIO model makes it possible for the decision-maker to make flexible strategies to balance the CEUP target and saving system costs. The results have demonstrated the validity of the PCIO model in addressing the hierarchical programming problems.
Laser Processing Optimization for Large-Area Perovskite Solar Modules
Authors: Razza Stefano , Pescetelli Sara , Agresti Antonio , Di Carlo Aldo
Published: February 2021

The industrial exploitation of perovskite solar cell technology is still hampered by the lack of repeatable and high-throughput fabrication processes for large-area modules. The joint efforts of the scientific community allowed to demonstrate high-performing small area solar cells; however, retaining such results over large area modules is not trivial. Indeed, the development of deposition methods over large substrates is required together with additional laser processes for the realization of the monolithically integrated cells and their interconnections. In this work, we develop an efficient perovskite solar module based on 2D material engineered structure by optimizing the laser ablation steps (namely P1, P2, P3) required for shaping the module layout in series connected sub-cells. We investigate the impact of the P2 and P3 laser processes, carried out by employing a UV pulsed laser (pulse width = 10 ns; λ = 355 nm), over the final module performance. In particular, a P2 process for removing 2D material-based cell stack from interconnection area among adjacent cells is optimized. Moreover, the impact of the P3 process used to isolate adjacent sub-cells after gold realization over the module performance once laminated in panel configuration is elucidated. The developed fabrication process ensures high-performance repeatability over a large module number by demonstrating the use of laser processing in industrial production.
Smarter Together: Progressing Smart Data Platforms in Lyon, Munich, and Vienna
Authors: Morishita-Steffen Naomi , Alberola Rémi , Mougeot Baptiste , Vignali Étienne , Wikström Camilla , Montag Uwe , Gastaud Emmanuel , Lutz Brigitte , Hartmann Gerhard , Pfaffenbichler Franz , Hainoun Ali , Gaiddon Bruno , Marvuglia Antonino , Andreucci Maria
Published: February 2021

In a context where digital giants are increasingly influencing the actions decided by public policies, smart data platforms are a tool for collecting a great deal of information on the territory and a means of producing effective public policies to meet contemporary challenges, improve the quality of the city, and create new services. Within the framework of the Smarter Together project, the cities of Lyon (France), Munich (Germany), and Vienna (Austria) have integrated this tool into their city’s metabolism and use it at different scales. Nevertheless, the principle remains the same: the collection (or even dissemination) of internal and external data to the administration will enable the communities, companies, not-for-profit organizations, and civic administrations to “measure” the city and identify areas for improvement in the territory. Furthermore, through open data logics, public authorities can encourage external partners to become actors in territorial action by using findings from the data to produce services that will contribute to the development of the territory and increase the quality of the city and its infrastructure. Nevertheless, based on data that is relatively complex to extract and process, public data platforms raise many legal, technical, economic, and social issues. The cities either avoided collecting personal data or when dealing with sensitive data, use anonymized aggregated data. Cocreation activities with municipal, commercial, civil society stakeholders, and citizens adopted the strategies and tools of the intelligent data platforms to develop new urban mobility and government informational services for both citizens and public authorities. The data platforms are evolving for transparent alignment with 2030 climate-neutrality objectives while municipalities strive for greater agility to respond to disruptive events like the COVID-19 pandemic.
Controlled Salinity-Biosurfactant Enhanced Oil Recovery at Ambient and Reservoir Temperatures—An Experimental Study
Authors: Udoh Tinuola , Vinogradov Jan
Published: February 2021

In this paper, a thorough experimental investigation of enhanced oil recovery via controlled salinity-biosurfactant injection under typical reservoir temperature conditions is reported for the first time. Sixteen core flooding experiments were carried out with four displacing fluids in carbonate rock samples and the improved oil recovery was investigated in secondary, tertiary and quaternary injection modes. The temperature effect on oil recovery during floodings was compared at two temperatures (23 °C and 70 °C) on similar rock samples and fluids using two types of biosurfactants: GreenZyme® and rhamnolipids. The results of this study show that injection of controlled salinity brine (CSB) and controlled salinity biosurfactant brine (CSBSB) improve oil recovery relative to injection of high salinity formation brine (FMB) at both high and low temperatures. At 23 °C, CSBSB improved oil recovery by 15–17% OIIP compared with conventional FMB injection, and by 4–8% OIIP compared with CSB injection. At 70 °C, the injection of CSBSB increased oil recovery by 10–13% OIIP compared with injection of FMB, and by 2–6% OIIP compared with CSB injection. Furthermore, increase in the system temperature generally resulted in increased oil recovery, irrespective of the type of the injection brine. The results of this study have demonstrated for the first time the enhanced oil recovery potential of combined controlled salinity brine and biosurfactant applications at temperature relevant to hydrocarbon reservoirs. The results of this study are significant for the design of controlled salinity and biosurfactant flooding in carbonate reservoirs.
Evaluation of Medium Voltage Network for Propagation of Supraharmonics Resonance
Authors: Sudha Letha Shimi , Delgado Angela Espin , Rönnberg Sarah K. , Bollen Math H. J.
Published: February 2021

Power converters with high switching frequency used to integrate renewable power sources to medium and low voltage networks are sources of emission in the supraharmonic range (2 to 150 kHz). When such converters are connected to a medium voltage (MV) network these supraharmonics propagate through the MV network and can impact network and customer equipment over a wide range. This paper evaluates an existing Swedish MV electrical network and studies the pattern of supraharmonic resonance and the propagation of supraharmonics. The MV network consists of eight feeders including a small wind farm. Simulations reveal that, the bigger the MV network, the more resonant frequencies, but also the lower the amplitude of the resonance peaks in the driving point impedance. It was also identified that for short feeders as length increases, the magnitude of the transfer impedance at supraharmonic frequency decreases. For further increment in feeder length, the magnitude increases or becomes almost constant. For very long feeders, the transfer impedance further starts decreasing. The eight feeders in the network under study are similar but show completely different impedance versus frequency characteristics. Measurements at the MV side of the wind farm show time varying emissions in the supraharmonic range during low power production. The impact of these emissions coupled with system resonance is examined.
Estimation of the Compressive Strength of Corrugated Cardboard Boxes with Various Perforations
Authors: Garbowski Tomasz , Gajewski Tomasz , Grabski Jakub Krzysztof
Published: February 2021

This paper presents a modified analytical formula for estimating the static top-to-bottom compressive strength of corrugated board packaging with different perforations. The analytical framework is based here on Heimerl’s assumption with an extension from a single panel to a full box, enhanced with a numerically calculated critical load. In the proposed method, the torsional and shear stiffness of corrugated cardboard, as well as the panel depth-to-width ratio is implemented in the finite element model used for buckling analysis. The new approach is compared with the successful though the simplified McKee formula and is also verified with the experimental results of various packaging designs made of corrugated cardboard. The obtained results indicate that for boxes containing specific perforations, simplified methods give much larger estimation error than the analytical–numerical approach proposed in the article. To the best knowledge of the authors, the influence of the perforations has never been considered before in the analytical or analytical–numerical approach for estimation of the compressive strength of boxes made of corrugated paperboard. The novelty of this paper is to adopt the method presented to include perforation influence on the box compressive strength estimation.
Correlation of Environmental Parameters and the Water Saturation Induced Deterioration of Earthen Archaeological Sites: The Case of World Heritage Liangzhu City, China
Authors: Dong Mei , Hu Hui , Guo Qingling , Gong Xiaonan , Azzam Rafig , Kong Mengyue
Published: February 2021

This paper proposes a combined methodology for the quantitative analysis of the correlations between the monitored influencing environmental factors and the water saturation induced deterioration of earthen relics in a humid area. The Archaeological Ruins of Liangzhu City that have been exposed and severely damaged in a humid environment with high water content and dry–wet cycles are chosen as examples. A monitoring system including atmospheric, groundwater, soil moisture conditions, and images of the surface was installed. Based on the proposed methodology, 11 key influencing indexes involving groundwater, soil moisture and temperature at different depths, atmospheric radiation, and rainfall for the water saturation induced deterioration are investigated, and their correlation is described by a regression model. The weight rankings of influencing factors to the deterioration of the research area are calculated. The results can help quantitatively control the atmospheric environment where the earthen relics are located and can promote the conservation of the archaeological ruins in the humid environment.
The Influence of Lockdown on the Gambling Pattern of Swiss Casinos Players
Authors: Lischer Suzanne , Steffen Angela , Schwarz Jürg , Mathys Jacqueline
Published: February 2021

The coronavirus pandemic (COVID-19) has had a major impact on most societies worldwide, including the closure of non-essential businesses in spring 2020. The present study considers its impact upon gambling behavior. Particularly, changes in self-reported gambling by Swiss, land-based casino players are examined. The main characteristics of respondents who played or did not play during lockdown are also investigated. This study is embedded in an ongoing longitudinal study that examines the gambling behavior of casino players at three points in time. All respondents who had participated in the first wave of the longitudinal study by the cut-off date (15th March 2020) were asked about their gambling behavior during lockdown in a supplementary online survey three weeks after the end of lockdown. A total of 55% of the 110 respondents reported having played during lockdown. Gambling intensity significantly decreased (p < 0.001) in our sample. Considering only those respondents (n = 66) who reported having gambled during lockdown, gambling intensity also decreased (p < 0.001), but online gambling significantly increased (p < 0.002). Those players who have increased their gambling activity require particular attention. It is important that casinos respond with appropriate player protection measures to those who have increased their gambling activity during the pandemic.
Ultrasound Imaging Evaluation of Textural Features in Athletes with Soleus Pathology—A Novel Case-Control Study
Authors: De-la-Cruz-Torres Blanca , Navarro-Flores Emmanuel , López-López Daniel , Romero-Morales Carlos
Published: February 2021

Background: the aim of this study was to compare the echotexture of patients with soleus muscle injury and age matched controls. Methods: a sample of 62 athletes was recruited at the private clinic and was divided in two group: a healthy group (n = 31) and a soleus pathology group whose athletes had soleus muscle injury, located in the central tendon (n = 31). The muscle thickness (MTh), echointensity (EI) and echovariation (EV) were analyzed. An intra-rater reliability test (Intraclass Correlation Coefficient-ICC) was performed in order to analyze the reliability of the values of the measurements. Results: Sociodemographic variables did not show statistically significant differences (p > 0.05). Ultrasound imaging measurements who reported statistically significant differences were EI (p = 0.001) and standard deviation (SD) (p = 0.001). MTh and EV variables did not show statistically significant differences (p = 0.381 and p = 0.364, respectively). Moreover, reliability values for the MTh (ICC = 0.911), EI (ICC = 0.982), SD (ICC = 0.955) and EV (ICC = 0.963). Based on these results the intra-rater reliability was considered excellent. Conclusion: Athletes with a central tendon injury of soleus muscle showed a lower EI when they were compared to healthy athletes. The echogenicity showed by the quantitative ultrasound imaging measurement may be a more objective parameter for the diagnosis and follow-up the soleus muscle injuries.
The Effects of Differences in the Morphologies of the Ulnar Collateral Ligament and Common Tendon of the Flexor-Pronator Muscles on Elbow Valgus Braking Function: A Simulation Study
Authors: Ikezu Masahiro , Edama Mutsuaki , Inai Takuma , Matsuzawa Kanta , Kaneko Fumiya , Hirabayashi Ryo , Kageyama Ikuo
Published: February 2021

The anterior bundle (AB) and posterior bundle (PB) of the ulnar collateral ligament and the anterior common tendon (ACT) and posterior common tendon (PCT) of the flexor-pronator muscles have an independent form and an unclear form. The purpose of this study was to clarify the effect of differences in the morphologies of the AB, PB, ACT, and PCT on the elbow valgus braking function. This investigation examined three elbows. In the classification method, the AB, PB, ACT, and PCT with independent forms constituted Group I; the AB, ACT, and PCT with independent forms and the PB with an unclear form constituted Group II; the AB, PB, ACT, and PCT with unclear forms constituted Group III. The strains were calculated by simulation during elbow flexion at valgus at 0° and 10°. At 0° valgus, Group I and Group II showed similar AB and PCT strain patterns, but Group III was different. At 10° valgus, most ligaments and tendons were taut with increasing valgus angle. The average strain patterns of all ligaments and tendons were similar for the groups. The AB, PB, ACT, and PCT may cooperate with each other to contribute to valgus braking.
Depression and Anxiety-Free Life Expectancy by Sex and Urban–Rural Areas in Jiangxi, China in 2013 and 2018
Authors: Wu Yuhang , Zheng Huilie , Liu Zhitao , Wang Shengwei , Chen Xiaoyun , Yu Huiqiang , Liu Yong , Hu Songbo
Published: February 2021

Objective: To quantitatively estimate life expectancy (LE) and depression and anxiety-free life expectancy (DAFLE) for the years 2013 and 2018 in Jiangxi Province, China, by sex and urban–rural areas. Additionally, to compare the discrepancy of DAFLE/LE of different sexes and urban-rural areas over various years. Methods: Based on the summary of the health statistics of Jiangxi Province in 2013 and 2018 and the results of the 5th and 6th National Health Service Surveys in Jiangxi Province, the model life table is used to estimate the age-specific mortality rate by sex and urban–rural areas. Sullivan’s method was used to calculate DAFLE. Results: Data from 2013 indicate that those aged 15 can expect to live 56.20 years without depression and anxiety for men and 59.67 years without depression and anxiety for women. Compared to 2013, DAFLE had not fluctuated significantly in 2018. The proportion of life expectancy without depression and anxiety (DAFLE/LE) declined between 2013 and 2018. DAFLE/LE in urban areas was higher than in rural areas. Men had higher DAFLE/LE than women. From 2013 to 2018, the DAFLE aged 15 decreased by 0.18 years for urban men and decreased by 0.52 years for urban women, rural areas also decreased to varying degrees. Conclusions: Even if women had a longer life span than men, they would spend more time with depression or anxiety. DAFLE did not increase with the increase in LE from 2013 to 2018, suggesting an absolute expansion of the burden, especially in rural areas. Depression and anxiety health services in Jiangxi, China will face more serious obstacles and challenges, which may lead to more disability. This requires more attention and more effective measures from the public, medical departments and the government.
Projecting Health Impacts of Future Temperature: A Comparison of Quantile-Mapping Bias-Correction Methods
Authors: Qian Weijia , Chang Howard H.
Published: February 2021

Health impact assessments of future environmental exposures are routinely conducted to quantify population burdens associated with the changing climate. It is well-recognized that simulations from climate models need to be bias-corrected against observations to estimate future exposures. Quantile mapping (QM) is a technique that has gained popularity in climate science because of its focus on bias-correcting the entire exposure distribution. Even though improved bias-correction at the extreme tails of exposure may be particularly important for estimating health burdens, the application of QM in health impact projection has been limited. In this paper we describe and apply five QM methods to estimate excess emergency department (ED) visits due to projected changes in warm-season minimum temperature in Atlanta, USA. We utilized temperature projections from an ensemble of regional climate models in the North American-Coordinated Regional Climate Downscaling Experiment (NA-CORDEX). Across QM methods, we estimated consistent increase in ED visits across climate model ensemble under RCP 8.5 during the period 2050 to 2099. We found that QM methods can significantly reduce between-model variation in health impact projections (50–70% decreases in between-model standard deviation). Particularly, the quantile delta mapping approach had the largest reduction and is recommended also because of its ability to preserve model-projected absolute temporal changes in quantiles.
Expression Profiling Identifies TWIST2 Target Genes in Setleis Syndrome Patient Fibroblast and Lymphoblast Cells
Authors: Crespo Noe E. , Torres-Bracero Alexandra , Renta Jessicca Y. , Desnick Robert J. , Cadilla Carmen L.
Published: February 2021

Background: Setleis syndrome (SS) is a focal facial dermal dysplasia presenting with bilateral temporal skin lesions, eyelash abnormalities and absent meibomian glands. SS is a rare autosomal recessive disorder caused by mutations in the TWIST2 gene, which codes for a transcription factor of the bHLH family known to be involved in skin and facial development. Methods: We obtained gene expression profiles by microarray analyses from control and SS patient primary skin fibroblast and lymphoblastoid cell lines. Results: Out of 983 differentially regulated genes in fibroblasts (fold change ≥ 2.0), 479 were down-regulated and 509 were up-regulated, while in lymphoblasts, 1248 genes were down-regulated and 73 up-regulated. RT-PCR reactions confirmed altered expression of selected genes. Conclusions: TWIST2 is described as a repressor, but expression profiling suggests an important role in gene activation as well, as evidenced by the number of genes that are down-regulated, with a much higher proportion of down-regulated genes found in lymphoblastoid cells from an SS patient. As expected, both types of cell types showed dysregulation of cytokine genes. These results identify potential TWIST2 target genes in two important cell types relevant to rare disorders caused by mutations in this bHLH gene.
The Validation of Direct and Meta Versions of the Coach–Athlete Relationship Questionnaire (ArCART-Q) in the Arabic Language: Their Relationship to Athlete’s Satisfaction with Individual Performance
Authors: Ahmad Hasan , Ada Elif Nilay , Jowett Sophia , Alabduljader Kholoud , Kazak Zişan
Published: February 2021

Background: The first aim of this study is to achieve validation of the direct and meta-perspective versions of the Coach–Athlete Relationship Questionnaire in the Arabic language, and the second aim of this study is to determine the quality of the coach-athlete relationship to athlete’ satisfaction with individual performance according to sport participation type, sport duration, and sport achievement. Methods: A total of 259 athletes with a mean age of 22 years were recruited from various athletic clubs in Kuwait. Participants completed The Coach–Athlete Relationship Questionnaire and The Athlete Satisfaction Questionnaire. For this study, the factorial structure of the Arabic version of the Coach–Athlete Relationship Questionnaire (CART-Q) was used in Kuwait and was prepared with both direct and meta perspectives. Results: The results of this study show evidence of the validity of the direct and meta-perspective Arabic versions of the CART-Q. The fit indices of the data collected by direct-method were as follows (x2/df = 2.21; RMSEA = 0.06; CFI = 0.98; GFI = 0.95; AGFI = 0.91); data for the meta-method were as follows (x2/df = 2.32; RMSEA = 0.08; CFI = 0.99; GFI = 0.93; AGFI = 0.87). Female participants have obtained higher means than males from all questionnaires. Conclusions: The results of the present study could help coaches and athletes from the Middle East to understand the reasons and methods that lead to a quality coach–athlete relationship.
Examining the Intended and Unintended Impacts of Raising a Minimum Legal Drinking Age on Primary and Secondary Societal Harm and Violence from a Contextual Policy Perspective: A Scoping Review
Authors: Roodbeen Ruud T. J. , Dijkstra Rachel I. , Schelleman-Offermans Karen , Friele Roland , van de Mheen Dike
Published: February 2021

Raising a minimum legal drinking age (MLDA) has generated interest and debate in research and politics, but opposition persists. Up to now, the presentation of impacts focussed on effectiveness (i.e., intended impact); to our knowledge, no literature syntheses focussed on both intended and unintended impacts. A systematic scoping review was conducted in which a search strategy was developed iteratively and literature was obtained from experts in alcohol research and scientific and grey databases. Ninety-one studies were extracted and analysed using formative thematic content analysis. Intended impacts were reported in 119 units of information from the studies (68% positive), forming four paths: implementation, primary and (two) on secondary societal harm and violence. Unintended developments were reported in 43 units of information (30% positive), forming five themes. Only eight studies reported on implementation. Furthermore, a division between primary and secondary paths and the use of a bridging variable (drinking patterns in analyses or methodology) was discovered. These results provide an insight into how well legislation works and can be used to discover or implement new means of curbing underage drinking and alcohol-related violence and harm. They also offer valuable starting points for future research and underline the importance of considering unintended developments.
Extreme Temperatures and Firm-Level Stock Returns
Authors: He Jingbin , Ma Xinru
Published: February 2021

By linking stock returns with weather conditions from 2007 to 2019 in China, we study how firm-level stock returns react to extreme temperatures. Based on a multivariate ordinary least squares regression model with fixed effects, empirical results show that firm-level stock returns decrease with exposure to extreme temperatures. We further explore the heterogeneity in the temperature-return relation to enrich our understanding of the economic mechanism behind it. The impact of extreme temperatures on abnormal stock returns is more pronounced in smaller, younger, more volatile, less profitable firms and firms with more intangible assets. The results indicate that the investor mood likely plays a role in the extreme temperature effect. The impact of extreme temperatures holds after addressing a series of concerns. Overall, our paper provides additional firm-level evidence on the environment-induced mood effect in the stock market.
A Systematic Review on Cross-Cultural Comparative Studies of Sleep in Young Populations: The Roles of Cultural Factors
Authors: Jeon Mina , Dimitriou Dagmara , Halstead Elizabeth J.
Published: February 2021

Recent studies have shown that sleep is influenced and shaped by cultural factors, including cultural values, beliefs and practices. However, a systematic understanding of how cultural factors in countries may influence sleep duration and sleep disturbances is still lacking. Therefore, we focused on a comparison of sleep duration and disturbances in young populations between countries. We report cross-cultural differences between the child, parent and environmental factors, and their association with sleep duration and disturbances. The review is based on literature searches of seven databases published until December 2020. Studies were included if they investigated sleep duration and disturbances of individuals up to 18 years across at least two or more countries. The results of this review have shown that sleep duration and disturbances vary between countries and regions and certain factors (e.g., bedtime routines, sleeping arrangement, physical activity and psychological functioning) have been associated with sleep duration or disturbances. This review also demonstrates that certain factors which were associated with sleep duration or disturbances in one country, were not shown in other countries, suggesting a need for recommendations for age-related sleep duration and sleep interventions to consider cultural differences that influence sleep duration or disturbances in individual countries or regions.
Impact of Food Additive Titanium Dioxide on Gut Microbiota Composition, Microbiota-Associated Functions, and Gut Barrier: A Systematic Review of In Vivo Animal Studies
Authors: Rinninella Emanuele , Cintoni Marco , Raoul Pauline , Mora Vincenzina , Gasbarrini Antonio , Mele Maria Cristina
Published: February 2021

Background: Titanium dioxide (TiO2) is used as a food additive in pastries, sweets, and sauces. It is recognized as safe by food safety authorities, but in recent years, governments and scientists have raised concerns about its genotoxicity. This systematic review aims to assess the potential associations between food TiO2 exposure and microbiota composition and functions. Methods: A systematic literature search was performed up to December 2020 in PubMed, Web of Science, and Scopus databases. The PRISMA guidelines followed. The risk of bias was assessed from ARRIVE and SYRCLE tools. Results: A total of 18 animal studies were included (n = 10 mice, n = 5 rats, n = 2 fruit flies, n = 1 silkworm). Studies varied significantly in protocols and outcomes assessment. TiO2 exposure might cause variations in abundance in specific bacterial species and lead to gut dysfunctions such as a reduction in SCFAs levels, goblet cells and crypts, mucus production, and increased biomarkers of intestinal inflammation. Conclusions: Although the extrapolation of these results from animals to humans remains difficult, this review highlights the key role of gut microbiota in gut nanotoxicology and stimulates discussions on the safe TiO2 use in food and dietary supplements. This systematic review was registered at PROSPERO as CRD42020223968.