bluuebunny/crossref_metadata_2025 huggingface dataset:
Expanding the application of haplotype-based genomic predictions to the wild: A case of antibody response against Teladorsagia circumcincta in Soay sheep
Authors: Vahedi Seyed Milad , Ardetani Siavash Salek , Brito Luiz Fernando , Karimi Karim , Afshar Kian Pahlavan , Banabazi Mohammad Hossein
Published: December 2022

Background
Genomic prediction of breeding values (GP) has been adopted in evolutionary genomic studies to uncover microevolutionary processes of wild populations or improve captive breeding strategies. While recent evolutionary studies applied GP with individual single nucleotide polymorphism (SNP), haplotype-based GP could outperform individual SNP predictions through more capture of the linkage disequilibrium (LD) between the SNP and quantitative trait loci (QTL). This study aimed to compare the accuracy and bias of Genomic Best Linear Unbiased Prediction (GBLUP) and five Bayesian methods [BayesA, BayesB, BayesCπ, Bayesian Lasso (BayesL), and BayesR] for GP of immunoglobulin (Ig) A (IgA), IgE, and IgG against Teladorsagia circumcincta in lambs of an unmanaged sheep population (Soay breed). Genomic predictions using SNP, haplotypic pseudo-SNP from blocks with different LD thresholds (0.15, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.00), or the combinations of pseudo-SNPs and non-LD clustered SNPs were evaluated.
Results
Higher ranges of accuracies were observed for IgA (0.36 to 0.82), followed by IgE (0.20 to 0.60), and IgG (0.09 to 0.33). For IgA, up to 33% gain in GP accuracy was obtained using the combinations of the pseudo-SNPs with non-clustered SNPs compared to GBLUP using SNPs. For IgE and IgG, up to 17% and 22% gains in accuracy were achieved by haplotype-based GPs compared to GBLUP using SNPs, respectively. Among haplotype-based GPs of IgA, lower accuracies were obtained with higher LD thresholds, whereas a reverse trend was observed for IgE and IgG. Bayesian methods outperformed GBLUP; BayesB achieved the most accurate Genomic Estimated Breeding Values (GEBV) for IgA (0.82) and IgG (0.33) and BayesCπ for IgE (0.60). Haplotype-based GPs predicted less-biased GEBVs in most IgG scenarios with high LD thresholds compared with SNP-based GBLUP, whereas no improvement in bias was observed for other traits.
Conclusions
Haplotype-based methods improved GP accuracy of anti-helminthic antibody traits compared to GBLUP using individual SNP. The observed gains in the predictive performances indicate that haplotype-based methods could be advantageous for some traits in unmanaged wild animal populations.
Health-related quality of life in migraine and non-migraine elderly using EQ-5D visual analog scale and utility index values
Authors: Fayegh Asieh Kouhi , Sulaiman Wan Aliaa Wan , Basri Hamidon , Ravanipour Maryam , Mohamed Mohd Hazmi , Din Hazwan Mat , Mat Liyana Najwa Inche
Published: December 2022

Background:The EQ-5D was used to compare the quality of life between the elderly with migraine and without migraine.Methods:A comparative cross-sectional survey of elderly participants was conducted in neurology clinics located in Tehran, Iran for a period of 2 years. The sample size was calculated as 186, including 63 migraine and 126 non-migraine groups. Mean EQ-5D scores were compared across groups using paired t-tests. We also compare the sociodemographic profiles of the 2 groups.Results:Apart from age, gender and occupation status there is no significant difference in marital status and education level between migraine and non-migraine groups. Mean EQ-5D scores were lower in elderly migraineurs. Moreover, there was a significant difference in all components of quality of life including mobility, self-care, usual activities, pain/discomfort and anxiety/depression as well as health utility index between the two groups of elderly with and without migraine.Conclusion:Migraine patients had lower EQ-5D scores compared to the non-migraine group, however, the elderly reported lower quality of life in general.
Monkeypox and cancer: a pan-cancer based multi-omics analysis and single cell sequencing analysis
Authors: Huang Xiaoliang , Long Xingqing , Liu Yanling , Chen Zuyuan , Xiang Xiaoyun , Mo Xianwei , Liu Jungang , Tang Weizhong
Published: December 2022

Background: Monkeypox is a zoonotic disease caused by monkeypox virus, and most infections cause systemic disease. Tumor patients are susceptible to various viruses, but there are few reports on the effects of genes related to monkeypox virus infection on the tumor microenvironment. Therefore, we need to further explore the expression of genes associated with monkeypox infection in tumor patients and the potential immune mechanisms in order to improve the survival and prognosis of tumor patients.
Methods: The gene expression, genetic variation, mRNA sequencing samples, clinical and methylation datas were from 33 cancer types of TCGG database. Single-cell transcriptome sequencing (scRNA-seq) was used to analyze the activation of monkeypox related genes in the tumor microenvironment. Then, Single sample gene set enrichment analysis (ssGSEA) and immunohistochemistry (IHC) were used in our research. The relationship between monkeypox and gene expression, clinical features, immune microenvironment, TMB and MSI was comprehensively evaluated.
Results: In this study, monkeypox-related genes were found to be closely associated with most tumorigenesis as well as drug sensitivity. Additionally, cellular enrichment pathways suggest that monkeypox is closely associated with invasion, cell cycle, DNA damage and repair. The prognostic value on monkeypox-related genes was evaluated, and it was found that monkeypox is a risk factor. Moreover,monkeypox-related genes are positively associated with immune cells as well as immune checkpoints in most tumors. Analysis of scRNA-seq indicates that monkeypox differs significantly among cells of the tumor microenvironment.
Conclusion: The current study explored the monkeypox-related genes in pan-cancer for the first time and provides new insights. We found that monkeypox-related genes are closely associated with the formation of immune microenvironments and immunotherapeutic efficacy of most tumor, which provides new ideas for the prevention and treatment of monkeypox and tumors.
Heronian Mean Operators Based on Complex Neutrosophic Uncertain Linguistic Sets and Their Application in Multi-Attribute Group Decision Making
Authors: Ali Zeeshan , Mahmood Tahir , Naeem Muhammad
Published: December 2022

The Heronian mean (HM) operator has the valuable characteristic of determining correlations among aggregated values. The HM operator is a massive feasible and more flexible technique to manage hurdle and awkward data in the circumstances of the fuzzy set (FS) theory. The major aims of this investigation are to analyze the novel theory of complex neutrosophic uncertain linguistic (CNUL) setting and their beneficial theories. The CNUL information includes uncertain linguistic sets, truth grade, abstinence grade, and falsity grade in the shape of complex numbers. In the occurrence of the novel concept of CNUL data, we try to invent the well-known theory of CNUL arithmetic HM (CNULAHM), CNUL weighted arithmetic HM (CNULWAHM), CNUL geometric HM (CNULGHM), CNUL weighted geometric HM (CNULWGHM) operators, which can help to aggregate the group of alternatives into a singleton element. Several attractive properties and their related results are also demonstrated. Additionally, to demonstrate the feasibility and rationality of the invented operators, multi-attribute group decision-making (MAGDM) performance is evaluated based on the invented works. Finally, we illustrated several examples to diagnose the sensitive analysis and graphically shown of the invented operators to express the consistency and feasibility of the evaluated works.
Relationship between silence behaviour, psychological empowerment, and patient safety culture among hospital staff: A multi-centre, cross-sectional study
Authors: Liu Hao , Zhang Hai-ying , Zhu Yong-kai , Huang Guo-ying , Xu Jia-hua
Published: December 2022

Background
As hospitals are under increasing pressure to improve quality and safety, safety culture has become a focal issue for high-risk organizations, including hospitals. Prior research has explored some of the factors that influence patient safety culture (PSC). However, there are relatively few related studies. In this study, we explored the relationship between silence behaviour, psychological empowerment, and PSC to find factors that affect the PSC in the hospital. Our study can serve as reference for hospital managers and for improving hospital service quality and patient safety.
Methods
The data for this study were from a multi-centre cross-sectional survey among hospital staff from six provinces/municipalities in China (N = 1034). Using correlation analysis and regression analysis to explore the impact of silence behaviour and psychological empowerment on PSC, we used a mediation model to explore the mediation effect among these three factors.
Results
Hospital staff PSC was correlated with silence behaviour and psychological empowerment. Furthermore, the effect of silence behaviour on PSC was mediated entirely through psychological empowerment. We found statistically significant differences in the perception of safety culture among respondents with different residential regions, sexes, education levels, and positions (P < 0.05).
Conclusions
Hospital managers should create an open and safe working atmosphere for hospital staff to for patient safety; improve the work system and mechanisms and open more communication channels to enhance the influence of employees. Reducing the punishment for mistakes, promoting a non-punitive safety culture through training, increasing the reporting frequency of adverse events, paying more attention to staffing, and paying attention to safety culture education of hospital administrative staff can effectively improve PSC and enhance patient safety.
UNINTENDED PREGNANCY AND ASSOCIATED FACTORS AMONG PREGNANT WOMEN LIVING WITH HIV BAHIRDAR TOWN PUBLIC HEALTH FACILITIES, NORTHWEST ETHIOPIA, 2022
Authors: Kefelegn Samuel , Aklilu Abayneh , Yetwale Fisseha , Amare Eyuel , Gashaw Anteneh
Published: December 2022

Background: Unintended pregnancy is a pregnancy that is untimely or unforeseen at the time of conception but may be wanted later.The burden and negative consequences of unintended pregnacy is miserable if it happens among HIV positive women.There was a paucity of evidence regarding unintended pregnancy among HIV positive women in the study area.
Objective:- To assess the prevalence and factor associated with unintended pregnancy among pregnant women living with HIV in Bahirdar town health care facilities 2022.
Methods:- An institution-based cross-sectional study was conducted from February 1st to march 30th 2022..A systematic sampling technique was employed to select 408 eligible women. A structured, pretested interview administered questionnaire and chart review was used to collect the data. Data were entered in to Epidata version 4.6 and exported to SPSS version 23 for data analysis. Multivariable logistic regression model fitted to identify factors associated with unintended pregnancy.Statical association was claimed based on the adjusted odds ratio (AOR) with its 95% CI and a p-value of ≤ 0.05.
Result :- Prevalence of unintended pregnancy among HIV positive pregnant women in Bahirdar town public health facilities was (22.4 %) with 95% CI=18.2- 26.4).Factors significantly associated with unintended pregnancy were, who doesn’t want more child [AOR:4.24,95%CI= 2.31-7.77], who don’t know about dual method [AOR:2.49,95%CI= 1.44-4.30] women taking ARV for more than five years [AOR:2.29,95%CI =1.32-3.95] and having non-regular sexual partner [AOR:0.30,95%CI=0.12-0.70) were significantly associated with unintended pregnancy.
Conclusion and recommendation:-Prevalence of unintended pregnancy among PWLHIV in Bahirdar town public health facilities was slightly less than the global unintended pregnancy prevalence. Better to give counselling on use of dual method and to practice safe sexual practice thereby decrease unintended pregnancy and reinfection with new virus.
Developing a Parenting Model for the Decent Use of Digital Media among the Teenagers in Bangladesh: A Qualitative Exploration
Authors: BakiBillah Abul Hasan , Khan Ayub , Muhammad Faisal , Chowdhury ABM Alauddin , Shahinuzzaman Md. , Hossain Syed Billal , Akram Muhammad , Almasri Nihad A. , Wani Mohammad Amin , Chowdhury Moniruddin , Ghosh Sanchita , Mukherjee Anindita , Kabir Russell
Published: December 2022

Background
In Bangladesh, parents are crucial in ensuring that teens use digital gadgets responsibly. The purpose of this study was to create a parenting paradigm within the context of the government's purges.
Method
Nineteen key informants took part in this study as part of the qualitative inquiry.
Results
Participants come from a variety of backgrounds and play a variety of roles that all support the same goal. The parenting model was created using the information from the interviews. By placing secondary and upper secondary educational institutions at the core of government action, parents will be educated. This approach was first proposed to be used in the "Generation Breakthrough" program of the Ministry of Education. Parents should be invited to the institutions to learn about the actions that need to be performed and followed under the two main categories, preventative and curative. Technology, the spoken word, psychology, and relationships built on evidence and trust are all examples of preventive approaches. Corrective measures include counseling, assistance with cyber security, legal services, and law enforcement.
Conclusion
In order to ensure that teens are using digital gadgets responsibly, this study suggests educating and empowering parents through a realistic strategy.
Molecular characterization and genetic diversity assessment in Mandukaparni (Centella asiatica L.) accessions
Authors: Singh Luwangshangbam James , Sane Anuradha , Thuppil Vasantha Kumar
Published: December 2022

Centella asiatica L. is an important herbaceous perennial medicinal plant commonly known as Mandukparni or Indian pennywort or Jalbrahmi or Gotu kola. It has been widely used in various traditional and modern medicine for its medicinal and nutritional properties. Although Centella asiatica L. is widely distributed throughout tropical and subtropical regions of India, only limited attempts have been made in exploiting diversity in conservation and crop improvement. Thus, the present study was aimed to assess the molecular diversity of its seventeen accessions by employing Simple Sequence Repeat markers. Among the twenty primers, eighteen primers showed seventy-five polymorphic bands which generated 97.40% polymorphism and two primers gave monomorphic bands across the accessions. The Polymorphism Information Content values ranged from 0.20 to 0.34 with an average of 0.26. The unweighted Pair Group Method with Arithmetic mean dendrogram grouped the accessions into two major clusters. The highest molecular distance shown at 56% in between the accessions (IIHR CA-5 and IIHR CA-16, IIHR CA-17) and (IIHR CA-8 and IIHR CA-17) revealed the most diverse pairs of accessions in comparison with other accessions. The results showed that Simple Sequence Repeat markers are suitable for assessing the genetic diversity of Centella asiatica accessions and the most diverse pairs of germplasms that is (IIHR CA-5 and IIHR CA 16, IIHR CA 17) and (IIHR CA-8 and IIHR CA 17) can be utilized in breeding programmes to widen the genetic base in this genetic crop improvement.
The clinical necessity of a distal forearm DEXA scan for predicting distal radius fracture in elderly females : a retrospective case-control study
Authors: Ma Sang Beom , Lee Sang Ki , An Young Sun , Kim Woo-suk , Choy Won Sik
Published: December 2022

Background:Recent studies have demonstrated that the distal forearm dual-energy X-ray absorptiometry (DEXA) scan might be a better method for screening bone mineral density (BMD) and the risk of distal forearm fracture, compared with a central DEXA scan. Therefore, the purpose of this study was to determine the effectiveness of a distal forearm DEXA scan for predicting the occurrence of distal radius fracture (DRF) in elderly females who were not initially diagnosed with osteoporosis after a central DEXA scan.Methods:Among the female patients who visited our institutes and who were over 50 years old and underwent DEXA scans at 3 sites (lumbar spine, proximal femur, and distal forearm), 228 patients with DRF (group 1) and 228 propensity score-matched patients without fractures (group 2) were included in this study. The patients’ general characteristics, BMD, and T-scores were compared. Odds ratios (OR) of each measurement and correlation ratio among BMD values of the different sites were evaluated.Results:The distal forearm T-score of the elderly females with DRF (group 1) was significantly lower than that of the control group (group 2) (p< 0.001 for the one third radius and ultradistal radius measurements). BMD measured during the distal forearm DEXA scan was a better predictor of DRF risk than BMD measured during the central DEXA (OR = 2.33;p= 0.031 for the one third radius, and OR = 3.98;p< 0.001 for the ultradistal radius). The distal one thirds radius BMD was correlated with hip BMD, rather than lumbar BMD (p< 0.05 in each group).Conclusion:Performing a distal forearm DEXA scan in addition to a central DEXA scan appears to be clinically significant for detecting the low BMD in the distal radius, which is associated with osteoporotic DRF in elderly females.Level of evidence:III; case-control study.
Multi-omics analysis to examine microbiota and metabolites in the colon of different breeds of swine
Authors: Zhu Yanbin , Sun Guangming , Cidan Yangji , Shi Bin , Tan Zhankun , Sun Fengbo , Ning Lihong , Han Haiyu , Wang Jinhui , Basang Wangdui
Published: December 2022

Background Host health and disease status are significantly influenced by the gut microbiome. However, the understandings of the gut microbiota in different breeds of pigs are largely unknown. Results In this study, six Duroc × landrace × yorkshire pigs and six Tibetan pigs were slaughtered to determine their short chain fatty acids (SCFAs) and microbes in colon and metabolites both in colon and plasma. The results revealed many variations between these two breeds of pigs. This study attempted to identify the major variables that explain the disparities in production performance between these two breeds of pigs. In our result, due to the significantly different relative abundances of microbes such asLachnospiraceae_NK4A136_group,AlloprevotellaandPrevotellaceae_UCG-003(P < 0.05), significantly higher concentrations of acetic acid, propionic acid and total SCFAs (P < 0.05), and significantly different concentrations of metabolites, duroc × landrace × yorkshire pigs showed better growth performance and health. Meanwhile, Tibetan pigs showed better abilities to use bile acid because of the higher relative abundances ofUCG-002andUCG-005, higher concentration of lithocholic acid and taurine, and higher expressions of bile acid receptors gene. Conclusion Based on the differences, the future studies could benefit these two breeds pigs’ production performance.
The effect of the vanillin-derived diene compound on the thermal, dielectric and mechanical properties of epoxy resins
Authors: yuan zhigang , Wang Lei , sun mingming , Zhang Xugang , Derradji Mehdi , zhang bin , Li Jianghui , Xue Gang , Zhao Ming , liu caizhao
Published: December 2022

In this work, a fluorine-containing diene compound (TFBAM) derived from vanillin was synthesized for modifying epoxy resin (E51). The chemical structure of TFBAM was confirmed by Fourier transform infrared (FTIR) and Nuclear magnetic resonance (1HNMR) spectroscopies. The modified resins (E-TFBAM) were successfully prepared through the introduction of TFBAM into E51, and the consequences of TFBAM dosages on the curing behavior, thermal, mechanical and dielectric properties of E-TFBAM resins were dissected in details. The results revealed that an appropriate concentration of TFBAM could be conductive to improving the thermal stability and dielectric properties of E-TFBAM thermosets without damaging the mechanical properties. The dielectric permittivity and loss of 2.82-2.71 and 0.024-0.015 were obtained when the addition of TFBAM was 30 wt%, with 10–10.2% and 36.8–44.4% of reduction. Additionally, the impact strengths and elongation at break of E-30 TFBAM resin increase to 18.0 kJ/m2 and 4.7%, respectively. Overall, this research can be seen as essential for expanding the application of the epoxy resins in high-end fields.
WITHDRAWN: A comprehensive Review of Innovative Human Resources Management
Authors: Atallah Ahmad
Published: December 2022

This study briefly reviews the research on novel approaches to HRM. Analysis of 47 articles yields three primary schools of thought when it comes to creative HRM. At its most basic, HRM innovation is just that. How human resource management handles novelty is the subject of other research. Thirdly, the term "innovative HRM" can be found in studies of inventiveness in business. Each of these three methods is discussed, along with their theoretical underpinnings. Implications for future study and practice are discussed at the end of the note.
WITHDRAWN: Upregulated HMGB3 resulting in the poor prognosis of lung adenocarcinoma by activating stromal angiogenesis through immune microenvironment
Authors: Feng Weirong , Bai Xiaoming
Published: December 2022

HMGB3 belongs to the high mobility group box (HMGB) family and the abnormally increased HMGB3 is associated with various types of malignancy progression. However, there is exiguous well clarified research to illustrate the correlation between HMGB3 and lung cancer. The expression profile data of lung adenocarcinoma (LUAD) were obtained from GEO and TCGA databases. The differential expression, prognostic survival, clinical characters, functional enrichment and immune infiltration were analyzed through R software, Kaplan–Meier (KM) plotter, ClusterProfiler package and ssGSEA algorithm. Besides, further research on HMGB3 expression level was confirmed using xenograft mice through immunohistochemistry and western blot assays. Results showed that HMGB3 was highly expressed in LUAD samples compared with adjacent normal samples. The T stage, pathologic stage, smoker and angiogenesis within the LUAD patients were positively correlated with HMGB3 expression. Functional enrichment analysis indicated that the correlative genes of HMGB3 are most related to the cell cycle process. HMGB3 is weakly related to the immune infiltration cells in LUAD stromal. However, increased existence of HMGB3 induced active angiogenesis in LUAD stromal and possibly accelerated the tumor progression through immune microenvironment. In conclusion, we confirmed that HMGB3 is a biomarker of poor prognosis for LUAD. The operative mechanism of which is activating tumor angiogenesis through immune infiltration cells. Further study will focus on exploring the related pathways in angiogenesis and immune microenvironment.
77GHz Down-Conversion Mixer with +18.4 dB High-Gain, +12.2 dBm OIP3, and Low-Noise in 90-nm CMOS Technology
Authors: Zhang Huan , Tang Sida , Cai Mengye , Jiang Yanfeng
Published: December 2022

In radio-frequency (RF) transceiver system, the receiver is used to convert RF signal to low/medium frequency for signal processing and information gathering. In the paper, a novel high-gain, low-noise CMOS mixer is designed and analyzed. In the design, gain-boosting and PMOS dynamic switching current are employed to achieve better effects of noise cancellation and trans-conductance enhancement. In order to improve the mixer's performance, the designed structure is mainly based on the double-balanced Gilbert lattice mixer combined with the parallel LC resonant and optimum biasing networks. The mixer shows high conversion gain (CG), low noise figure (NF), and low power consumption performances based on made possible by 90-nm CMOS technology. Operated at 77 GHz, the input third-order intercept point (IIP3) is -6.14 dBm, and the maximum conversion gain is 18.4 dB. At an IF frequency of 200 MHz, a bilateral band noise figure of 9.2 dB is recorded, while 6.96 mW is consumed with 1.2 V and 2 dBm LO power.
The use of a 30-degree radiolucent triangle during surgery in distal avulsion fractures of the patella
Authors: Ragot Léa , Gerber Filippo , Lannes Xavier , Moerenhout Kevin
Published: December 2022

Background
Avoiding patella baja or alta after the Krackow suture technique for distal avulsion fractures of the patella can be challenging. We aim to introduce a simple and reproducible technique using a 30-degree radiolucent triangle involving the contralateral knee to ensure the correct positioning of the patella intraoperatively.
Methods
The radiolucent triangle is positioned under the contralateral knee before operating the injured knee. A strict lateral view is obtained using fluoroscopy as a reference before a Krackow technique is performed on the avulsion fracture of the patella.
Results
The triangle technique is straightforward and easily reproducible by surgeons of all levels. It allows the surgeon to correctly position the patella intraoperatively in avulsion fracture repair and modify tension on the patellar tendon.
Conclusion
This method avoids millimetric mispositioning of the operated patella, thus improving the management intraoperatively and could decrease postoperative complications.
Experimental Study on Residual Deformation of Coal Gangue Filler under Multi-vibration Cyclic Loading
Authors: Xiaoyu Tang , Wen Wan , Zongtang Zhang , Wenhua Gao , Wang Yanhao
Published: December 2022

The use of coal gangue as roadbed filler can alleviate environmental problems and reduce environmental pollution. In this paper, the multi-vibration cyclic loading test of coal gangue roadbed filling body considering different confining pressure, compactness and gradation parameters is carried out. The deformation characteristics were studied, the residual deformation model was established, and the influence of different factors on the residual deformation value was discussed. The results show that under the cyclic loading of multiple vibration times, the deformation growth rate of coal gangue filler begins to increase, then slows down and tends to be constant. The actual residual deformation of coal gangue filler is not an accurate logarithmic relationship with the number of earthquakes. The shear and volumetric residual strains of 0 ~ 200 earthquakes have a quadratic function relation with logarithmic earthquake number lg (1 + N). There is a logarithmic relationship between the volume residual strain and the logarithmic number lg (1 + N) in 201 ~ 10000 earthquakes. All the influencing factors have a certain influence on the residual deformation value of the coal gangue specimen. According to the grey correlation degree calculation, the influence degree of the influencing factors of the coal gangue filler is ranked as the number of earthquakes, confining pressure, compaction degree and gradation. In addition, according to the grey correlation analysis, the influence of soil particle tumbling on the residual deformation is greater than the influence of soil particle crushing.
Combination therapy based on dual-target biomimetic nano-delivery system for overcoming cisplatin resistance in hepatocellular carcinoma
Authors: huang yufen , Kou Qinjie , Su Yanrong , Lu Lu , Li Xisheng , Jiang Haiye , Gui Rong , Huang Rong , Nie Xinmin , Li Jian
Published: December 2022

The clinical use of cisplatin (DDP) is severely restricted because of nonspecific delivery and the induction of serious systemic toxicity. The limited anticancer drug library and the frequent occurrence of multidrug resistance (MDR) have made monotherapy difficult. Strategies to overcome cisplatin resistance and reduce the long-term use of high-dose medicines, as well as strategies for targeted therapy against hepatocellular carcinoma (HCC) are urgently needed. Previous studies revealed that high NOR1 expression in HCC was associated with drug resistance. Herein, a novel, precise drug/siRNA delivery strategy to realize efficient combination chemotherapy-gene therapy is proposed. To pursue this aim, the dual-targeting nanocarrier system A54-RBCm@NLS-Ag-MOFs/DDP/NOR1 shRNA (AR-NADR) was constructed for the treatment of cisplatin resistance in HCC. The core of the nanocarrier system is the NLS peptide-modified Ag-MOFs (NA) synthesized by silver ion (Ag+) and L-histidine loaded with cisplatin and NOR1 shRNA (NADR). The shell part is the A54 peptide inserted into the erythrocyte membrane (AR). Our results show that AR-NADR has efficient internalization by tumor cells owing to its specific binding to the A54 receptors that are abundantly expressed on the surface of HCC cells and the NLS peptide-mediated pathway of nuclear entry. Simultaneously, as a novel smart pH-sensitive nanodrug delivery system (DDS), DDP is more likely to be released in the acidic tumor microenvironment. Moreover, acting as a vector for gene delivery, AR-NADR effectively inhibits tumor drug resistance by dramatically suppressing the expression of NOR1, which induces intracellular DDP accumulation and makes cells sensitive to DDP. Finally, the anti-HCC efficacy and mechanisms of AR-NADR were systematically elucidated by a HepG2/DDP cell model as well as a tumor model. AR-NADR exhibits critical antitumor efficiency and good biocompatibility. Therefore, AR-NADR constitutes a significant strategy to achieve excellent gene silencing efficacy and antitumor efficacy, which provides effective gene therapy and precise treatment strategies for cisplatin resistance in HCC.
WITHDRAWN: Highly improved UV-shielding and mechanical properties of UV-curable epoxy acrylate nanocomposites by incorporating functionalized aramid nanofibers
Authors: Wang Ying , Luo Yuexin , Pan Yuankai , Ji Chunnuan , Zhang Ying , Sun Changmei , Qu Rongjun
Published: December 2022

Introducing nanofillers into UV-curable coatings can not only effectively improve the performance of the coating but also impart new functions to the film. Herein, methacrylate-functionalized aramid nanofibers (mANFs) were constructed by a bottom-up approach, and their influence on the performance of UV-curable epoxy acrylate (EA) coatings was investigated. The microstructure of the EA/mANF nanocomposite was studied using X-ray Diffraction and Transmission Electron Microscopy (TEM), which confirmed the homogeneous distribution of mANFs in the matrix. The effect of mANFs on the curing kinetics was investigated. The result showed that the photopolymerization rate and final C = C bond conversion would not be adversely affected until more than 0.1 wt % mANFs were added. The UV–vis analyses showed that the EA/mANF films maintained high transmittance in the visible light region and exhibited improved UV-shielding performance. The mechanical characteristics of EA/mANF films were significantly improved by mANFs because of strong interactions such as π–π interactions, hydrogen bonds, and chemical bonds between the matrix and mANFs. For the nanocomposite containing 0.025 wt % mANFs, tensile strength and strain at break increased by 53% and 446%, respectively, compared to neat EA. Thus, the introduction of mANFs considerably improved the mechanical performance of UV-curable EA films and expanded their application in the field of UV shielding.
High Fidelity GAN Inversion with Condition Reiteration for Image Editing
Authors: Dere Vedant , Shinde Amita , Vast Prachi
Published: December 2022

We present an extension to High-Fidelity GAN Inversion(HFGI) that enables us to invert efficitevely image specificdetails (like background, appearance, etc.) in restoringand editing of normal images as well as out of the domainimages (e.g. Heavy makeup faces). We analyzed W+ latentcodes are low dimensional that harden to preserve imagespecific details in restoration. In HFGI encoder, singlestage conditional latents maps are used for invertingimages which restores image specific details with lowconfidence. So in a restored image the low confidenceregion becomes blurry. Also in editing images, detailedinformation is getting lost due to latent navigation. Hencewe propose a reiterative conditional latents method thatenables us to restore image specific details with sharpnesswithout changing the architecture of HFGI. The overallprocess consists of two stages of iterations. First stage willreconstruct the image and the second stage will work onimage specific details using conditional latent codes. Inediting images, we are applying an editor in the first stageonly which will edit latents and in the second stage imagespecific details becomes sharp by conditional latent maps.Our model is also able to invert out of the domain imageswhile preserving all the details. We support InterfaceGAN,GANspace, StyleClip for editing. Moreover we compare ourapproach with the state of the art GAN inversion methodson FFHQ and Stanford Cars Dataset, qualitatively andquantitatively. The results shows significant improvement ininversion and editing quality.
Adsorption of thioindole as a biologically active anti-cancer to C 20 fullerene in different reaction media using density functional theory
Authors: Mohammadi Marziyeh
Published: December 2022

The reaction media effects on interaction of commercially available asymmetric thioindole; (E)-3-(1H-pyrrole-2-yl)methylene-indoline-2-thione (I) as a selective and potent inhibitor of tyrosine kinase; to C20 nanocage are studied at DFT. We have tackled this inter-molecular adsorption using the PCM-SCRF method in the gas phase, cyclohexane, CHCl3, MeOH, H2O, acetonitrile and DMSO. We completely optimized the scrutinized initial structures, transition states (TSs) and product (Ia) in the different reaction media and then compared and contrasted their properties. Stability of I solute and Ia complex depends on the dielectric constant of the used solvent (ε), the possibility of the hydrogen bonding (HB) and dipole–dipole interaction between them. In going from the gas phase to less polar solvent, then in turn to more polar solvent, a good consistency appears between ε and the absolute value of adsorption energy difference among liquid phase and gas phase i.e. │∆El-g│ obtained for Ia product. Moreover, the highest │∆El-g│ is measured for Ia in water, whereas the lowest value is associated in cyclohexane. Because of capability of HB, Ia is stabilized in the most polar solvent (water) more than gas phase and other solvents. The kinetic stability and energy difference between the frontier orbitals (∆EL-H) is observed in the opposite direction with ε. The π-π aromatic stacking between I and C20 leads to destabilization of endo TS. Dipole-pole interaction between I and more polar solvent, leads to more stability of exo TS and Ia in water solvent. The activation energy of exo and endo TSs (Eexo≠ and Eendo≠) in the studied reaction media is followed as H2O > DMSO > acetonitrile > MeOH > CHCl3 > cyclohexane > gas phase. The highest Eexo≠ and Eendo≠ is found in water. Evidently, the most and the least kinetically stable exo and endo TS is suggested for adsorption in water and gas phase, respectively.
ScAlN film-based piezoelectric micromechanical ultrasonic transducers with dual-ring structure for distance sensing
Authors: Li Jiadong , zhang yuchao , Miao Bin , Wang Guanghua , Zhou Hongyu , Zhang Shiqin , Hu Yimin , Wu Junfeng , Yu Xuechao
Published: December 2022

Piezoelectric micromechanical ultrasonic transducers (pMUTs) are a new type of distance sensors with great potential for applications in automotive, unmanned aerial vehicle, robotics, and smart-home. However, the previously reported pMUT is limited by short sensing distance due to lower output sound pressure. In this work, a pMUT with a special dual-ring structure based on scandium-doped aluminum nitride (ScAlN) is proposed. The combination of a dual-ring structure with pinned boundary conditions and a high piezoelectric performance ScAlN film allows the pMUT to achieve a large dynamic displacement of 2.87 μm/V and a high electromechanical coupling coefficient (k2t) of 8.92%. The results of the ranging experiments show that the proposed pMUT achieves a 6 m distance sensing, the farthest distance sensing up to date registered. This pMUT provides surprisingly fertile ground for various distance sensing applications.
In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus
Authors: Maulana Rafiq Usdiqa , Wardani Agustin Krisna , Utami Cinthya Putri
Published: December 2022

Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. In sum, it suggested three compounds with the best potential for anti-MRSA candidates: curcumin, germacrone, and zerumbone and may be considered as drug candidates for therapeutic aims in several human infections associated with MRSA. Nevertheless, in vitro and in vivo confirmations are needed.
Accumulation of Na+ in cotton field under mulched drip irrigation of brackish water in arid areas
Authors: Dong Rui , Liu Wenkai , Qu Jihong
Published: December 2022

In arid areas, the disordered accumulation of Na+ in cotton fields under mulched drip irrigation seriously affects the normal growth and development of cotton. To reveal the process and mechanism of Na+ accumulation, in-situ field tests were conducted in typical cotton planting bases in the south of Xinjiang, China. Eight experimental scenarios were set up to use brackish and fresh water for drip irrigation under mulch during the whole growth period of cotton according to the drip irrigation system. By monitoring the weather, groundwater level, and soil moisture before and after irrigation, and testing the soil salinity and Na+ concentration, the temporal and spatial distribution and transport characteristics of Na+ were compared and studied. The results showed that: (1) owing to the strong spatial variation of soil permeability, soil water and salt dynamics exhibited significant differences after irrigation in all experimental scenarios in the study area, especially at a depth of 0–60 cm. (2) The Na+ profile exhibited a “thin waist”, whose depth depended on the climate and lithology of the soil; however, this phenomenon was not observed in the salt profile. (3) The accumulation of Na+ in cotton fields is an extremely complex dynamic process that is influenced by both natural and human activities. This process is controlled by the dynamic characteristics of soil water and salt, but it is different from the distribution of soil salt in time and space. This study provides necessary technical support for the formulation of drip irrigation systems under brackish water film, and provides a scientific basis for fine agricultural planting and water resources management in arid areas.
WITHDRAWN: HO-1 may be the potential therapeutic target for Atopic dermatitis as oxidative stress is dysregulated and associated with inflammation in Atopic dermatitis patients
Authors: Yuan Jinping , Wang Xiyi , Yu Huiwen , Xu Lei , Wu Xiaoyu , Mao Mengmeng , Bai Bing-Xue
Published: December 2022

Atopic dermatitis (AD) is a chronic inflammatory skin disease and oxidative stress plays pathogenetic role in it. Heme oxygenase-1 (HO-1) is a key enzyme in response to oxidative and inflammatory insults. This study aims to evaluate the correlation between HO-1 expression, oxidative stress and inflammation in AD patients, identify the antioxidative and anti-inflammation effects of HO-1 on H2O2-induced HaCaT cells. Forty-two AD patients and twelve healthy controls were enrolled. Human Oxidative Stress PCR Array was performed to test the oxidative stress related gene expression in AD patients and controls. The HO-1 expression, malondialdehyde (MDA) and SOD and inflammatory factors IL-4 and high mobility group box1 (HMGB1) were measured by ELISA. The effect of HO-1 on the expressions of ROS, MDA and SOD, and inflammatory factors IL-4 and TNF-α were also detected in H2O2-induced HaCaT cells. We demonstrated that the levels of MDA were increased while SOD, antioxidants genes, ROS metabolisms and oxygen transporter genes were decreased in AD patients than in controls. In addition, high expression of MDA is positively correlated with IL-4 expression and disease severity of AD. HMGB1was also positively correlated with SOD activity. Furthermore, the level of HO-1 was significantly higher in AD patients than controls and HO-1 increased the SOD activity and decreased the levels of ROS, MDA, decreased the expressions of IL-4 and TNF-α in H2O2 -induced oxidative stress in HaCaT cells. In conclusion, oxidative damage is positively correlated with inflammatory factors and disease severity of AD and HO-1 may be potential target as its antioxidant and anti-inflammation effects.
Reference Correlation for the Viscosity of Ethanol from the Triple Point to 620 K and Pressures up to 102 MPa
Authors: Sotiriadou Sofia , Ntonti Eleftheria , Velliadou Danai , Antoniadis Konstantinos D. , Assael Marc , Huber Marcia L.
Published: December 2022

We present a wide-ranging correlation expressed in terms of temperature and density for the viscosity of ethanol based on critically evaluated experimental data. The correlation is designed to be used with an existing equation of state from the triple point (159 K) to 620 K and at pressures up to 102 MPa. Comparisons with experimental data indicate the estimated uncertainty of the correlation is 4.2 % (at the 95% confidence level) for the liquid and supercritical phase at pressures up to 102 MPa, and 2% in the gas phase. Furthermore, for calculating viscosity values at 0.1 MPa, an additional correlation is proposed, valid from the triple point to the boiling point with an estimated uncertainty of 2.3 % (at the 95% confidence level).
The increased monocytic myeloid-derived suppressor cells in type 2 diabetes corelate with hyperglycemic and was a risk factor of infection and tumor
Authors: Zhou Ji , Zhang Mengjie , Ju Xiaodi , Wang Huiping , Hao Xiao , Zhai Zhimin , Zhong Xing , Hong Jingfang
Published: December 2022

Background
To explore the M-MDSCs frequency in T2DM patients and whether it is corelated to the glycaemia, infection and tumor development.
Methods
We recruited healthy volunteers and T2DM patients for this study. M-MDSCs frequency in the peripheral blood, FPG, HbA1c levels, and other relevant indicators were detected. T2DM patients were further divided into good glycaemic control (GGC) and poor control (PGC) groups, and each patient was followed up for at least 6 months after the M-MDSCs were tested. We then analysed and compared the M-MDSCs frequency in the healthy population to various subgroups of T2DM patients, as well as the associations between M-MDSCs, glycaemia, infection, and tumor development.
Results
The M-MDSCs frequency was significantly higher in T2DM patients with PGC than in the healthy population (2.54% vs 0.93%), but there was no significant difference between patients with GGC and the healthy group (P > 0.05). The M-MDSCs frequency was positively correlated with FPG and HbA1c levels (R = 0.517 and 0.315, respectively). In addition, the patients who had tumors had the highest M-MDSCs number (12.89%), vastly more than those in the patients who only had an infection (3.14%) and the patients who had neither infection nor tumor (1.95%). When M-MDSCs frequency was higher than 2.8% or 11.24%, the risk ratios for infection or tumor occurrence were 2.5-fold and 43.2-fold higher in T2DM patients, respectively.
Conclusions
Elevated M-MDSC levels are associated with hyperglycaemia and may be a useful indicator for predicting the risk of infection or tumor development in T2DM patients.
Mir-330-3p alleviates the progression of atherosclerosis through downregulating AQP9
Authors: Shan Erbo , Yu Yuanyuan , Tang Wenbo , Wang Wei , Wang Xiangkui , Zhou Shaobo , Gao Yong
Published: December 2022

Atherosclerosis is one of the main causes of cardiovascular diseases. Aquaporin 9 (AQP9), a triglyceride channel is permeable to water, monocarboxylic acids, glycerol and lactic acid. However, the role of AQP9 in atherosclerosis is not clear. In present study, we predicted that miR-330-3p might regulate AQP9 in atherosclerosis through bioinformatics analysis, then we established atherosclerosis model by ApoE −/− mouse (C57BL/6) with high fat diet (HFD). Hematoxylin and eosin (H&E) and Oil red O were used to stain atherosclerotic lesions. Furthermore, we used 100 µg/mL ox-LDL treat human umbilical vein endothelial cells (HUVECs) to investigate the role of miR-330-3p and AQP9. CCK8 and EDU assays were used to investigate the cell proliferation. Wound scratch healing and transwell assays were used to measure the cells invade and migrate ability. Flow cytometry assay was used to detect apoptosis and cell cycle changes. A dual-luciferase reporter assay was performed to determine the binding of miR-330-3p and AQP9. We identified that the expression of miR-330-3p in AS mice model decreased while the expression of AQP9 increased. MiR-330-3p overexpression or down-regulation of AQP9 could reduce cell apoptosis and promote cell proliferation and migration after ox-LDL treatment. Dual-luciferase reporter assay result presented that AQP9 is directly inhibited by miR-330-3p. These results suggest that miR-330-3p inhibits atherosclerosis by regulating AQP9. Our results indicates that miR-330-3p inhibits atherosclerosis by regulating AQP9. Mir-330-3p/AQP9 axis maybe a new therapeutic target for atherosclerosis.
Modeling and optimization of coagulation-flocculation process to remove high phosphate concentration in wastewater from a metal-mechanic industry
Authors: Gómez-Rodríguez Carlos , Gómez-Castro Fernando Israel , Gamiño-Arroyo Zeferino , Pérez Tzayam
Published: December 2022

In this work, the performance of a coagulation-flocculation plant to treat wastewater from a metal-mechanic industry located in an industrial park of Queretaro city, Mexico is studied. Wastewater samples were obtained from the homogenization tank and treated with the employed industrial reactants through an experimental jar test to obtain statistical data. Then, a response surface methodology with ANOVA analysis was used to model the process, and the ε-constraints methodology was used to optimize the coagulation-flocculation process in terms of economic and environmental impact. Optimal operating conditions were found at 400 mg L− 1 of calcium hydroxide dose, 0.723 mL L− 1 of aluminum salts dose, 3.32x10− 3 mg L− 1 flocculant dose and 100 rpm of agitation speed. The results showed an improvement of phosphates removal, but a minimal increment of 1.01% of operational costs regarding to the current operating conditions.