bluuebunny/crossref_metadata_2025 huggingface dataset:
Seismic Event Detection in the Copahue Volcano Based on Machine Learning: Towards an On-the-Edge Implementation
Authors: Sosa Yair Mauad , Molina Romina Soledad , Spagnotto Silvana , Melchor Iván , Nuñez Manquez Alejandro , Crespo Maria Liz , Ramponi Giovanni , Petrino Ricardo
Published: February 2024

This study focused on seismic event detection in a volcano using machine learning by leveraging the advantages of software/hardware co-design for a system on a chip (SoC) based on field-programmable gate array (FPGA) devices. A case study was conducted on the Copahue Volcano, an active stratovolcano located on the border between Argentina and Chile. Volcanic seismic event processing and detection were integrated into a PYNQ-based implementation by using a low-end SoC-FPGA device. We also provide insights into integrating an SoC-FPGA into the acquisition node, which can be valuable in scenarios where stations are deployed solely for data collection and holds the potential for the development of an early alert system.
Traffic Classification and Packet Scheduling Strategy with Deadline Constraints for Input-Queued Switches in Time-Sensitive Networking
Authors: Zheng Ling , Wei Guodong , Zhang Keyao , Chu Hongyun
Published: February 2024

Deterministic transmission technology is a core key technology that supports deterministic real-time transmission requirements for industrial control in Time-Sensitive Networking (TSN). It requires each network node to have a deterministic forwarding delay to ensure the real-time end-to-end transmission of critical traffic streams. Therefore, when forwarding data frames, the switch nodes must consider the time-limited requirements of the traffic. In the input-queued switch system, an algorithm for clock-synchronized deterministic network traffic classification scheduling (CSDN-TCS) is proposed to address the issue of whether a higher-quality-of-service (QoS) performance can be provided under packet deadline constraints. First, the scheduling problem of the switch is transformed into a decomposition problem of the traffic matrix. Secondly, the maximum weight-matching algorithm in graph theory is used to solve the matching results slot by slot. By fully utilizing the slot resources, as many packets as possible can be scheduled to be completed before the deadline arrives. For two types of packet scheduling problems, this paper uses the maximum flow algorithm with upper- and lower-bound constraints to move packets from a larger deadline set to idle slots in a smaller deadline set, enabling early transmission, reducing the average packet delay, and increasing system throughput. When there are three or more types of deadlines in the scheduling set, this scheduling problem is an NP-hard problem. We solve this problem by polling the two types of scheduling algorithms. In this paper, simulation experiments based on the switching size and line load are designed, and the Earliest Deadline First (EDF) algorithm and the Flow-Based Iterative Packet Scheduling (FIPS) algorithm are compared with the CSDN-TCS algorithm. The simulation results show that under the same conditions, the CSDN-TCS algorithm proposed in this paper outperforms the other two algorithms in terms of success rate, packet loss rate, average delay and throughput rate. Compared with the FIPS algorithm, the CSDN-TCS algorithm has lower time complexity under the same QoS performance.
A Compact Circular Waveguide Directional Coupler for High-Order Mode Vacuum Electronic Devices
Authors: Zhu Tao , Fu Wenjie , Lu Dun , Pan Yibo , Li Chuannan , He Lin , Sun Haoxuan , Yan Yang
Published: February 2024

In this paper, a compact circular waveguide directional coupler for high-order mode vacuum electronic devices is presented and investigated. To reduce the size, the primary and secondary waveguides of this coupler are connected in an orthogonal way by two coupling holes. Moreover, to improve the directivity and operating bandwidth, a method of loading adjustable metal stubs on the isolating port is proposed and introduced in design. A Ka-band TE01-mode circular waveguide directional coupler was designed, and the structure parameters were optimized by electromagnetic simulation. To verify the design, a prototype sample was fabricated, assembled, and tested. The experimental results show good agreement with the simulations, and the directivity are improved by adjusting the metal stubs on the isolating port. In the experiment, a 26.7 dB directivity at 35 GHz was obtained, and the bandwidth of directivity above 20 dB was higher than 7 GHz, corresponding to a relative bandwidth higher than 20%. Meanwhile, the TE01° mode maintained good transmission efficiency in this compact high-order mode directional coupler.
An Artificial Neural Network-Based Fault Diagnostics Approach for Hydrogen-Fueled Micro Gas Turbines
Authors: Hashmi Muhammad Baqir , Mansouri Mohammad , Fentaye Amare Desalegn , Ahsan Shazaib , Kyprianidis Konstantinos
Published: February 2024

The utilization of hydrogen fuel in gas turbines brings significant changes to the thermophysical properties of flue gas, including higher specific heat capacities and an enhanced steam content. Therefore, hydrogen-fueled gas turbines are susceptible to health degradation in the form of steam-induced corrosion and erosion in the hot gas path. In this context, the fault diagnosis of hydrogen-fueled gas turbines becomes indispensable. To the authors’ knowledge, there is a scarcity of fault diagnosis studies for retrofitted gas turbines considering hydrogen as a potential fuel. The present study, however, develops an artificial neural network (ANN)-based fault diagnosis model using the MATLAB environment. Prior to the fault detection, isolation, and identification modules, physics-based performance data of a 100 kW micro gas turbine (MGT) were synthesized using the GasTurb tool. An ANN-based classification algorithm showed a 96.2% classification accuracy for the fault detection and isolation. Moreover, the feedforward neural network-based regression algorithm showed quite good training, testing, and validation accuracies in terms of the root mean square error (RMSE). The study revealed that the presence of hydrogen-induced corrosion faults (both as a single corrosion fault or as simultaneous fouling and corrosion) led to false alarms, thereby prompting other incorrect faults during the fault detection and isolation modules. Additionally, the performance of the fault identification module for the hydrogen fuel scenario was found to be marginally lower than that of the natural gas case due to assumption of small magnitudes of faults arising from hydrogen-induced corrosion.
Zeolite and Winery Waste as Innovative By-Product for Vineyard Soil Management
Authors: Doni Serena , Masciandaro Grazia , Macci Cristina , Manzi Davide , Mattii Giovan Battista , Cataldo Eleonora , Gispert Maria , Vannucchi Francesca , Peruzzi Eleonora
Published: February 2024

In semiarid environments, vine cultivation is a land use with a high impact with regard to soil erosion, loss of organic matter and biodiversity, contamination, and compaction. In addition, the wine supply chain produces a considerable quantity of organic waste, which remains as residues in the ecosystem. Within this context, we developed a sustainable vine management system to improve the efficient use of fertilisers by applying a by-product derived from the composting of winery wastes and zeolite. We evaluated the effects of the zeolite-based compost on the chemical, physical, and biochemical soil properties of a productive vineyard. Four treatments were set up and monitored for about two years. These were as follows: (1) Commercial compost (COM); (2) Zeolite (Z); (3) 30% zeolite and 70% winery waste compost (30 ZEO); (4) 10% zeolite and 90% winery waste compost (10 ZEO). The results demonstrated that the ZEO treatments could be considered a win–win solution able to improve soil water content, nutrient retention, carbon sequestration, and biochemical activity while also recycling wastes. In particular, 10 ZEO seems to be the amendment that best combines an improvement in soil biochemical properties with gradual and constant nutrient availability, thus satisfying, without exceeding, soil and plant needs.
Hydraulic Traits and Non-Structural Carbon Responses to Drought Stress in Reaumuria soongorica (Pall.) Maxim. and Salsola passerina Bunge
Authors: Wang Hongyong , Ma Jing , Xie Tingting , Niu Furong , He Cai , Shi Yating , Zhang Zhengzhong , Zhang Jing , Shan Lishan
Published: February 2024

Drought-induced plant mortality, resulting from either hydraulic failure or carbon starvation, is hypothesized to be modulated by the drought intensity. However, there is a paucity of research investigating the response strategies in desert shrubs under drought stress with different intensities. We transplanted potted Reaumuria soongorica (Pall.) Maxim. and Salsola passerina Bunge seedlings in the rain-out shelter, and implemented three water treatments: a control (well-watered, CK), a chronic drought (gradually less watered, CD), and a flash drought (not watered, FD). We then quantified plant physiological traits associated with water use and carbon assimilation. Both R. soongorica and S. passerina showed similar changes in water use and carbon characteristics under different drought treatments. Water use efficiency was not significantly changed, but embolism resistance was significantly lower in CD, and leaf specific conductivity and embolism resistance were significantly lower in FD compared to CK. Under the drought treatment, both shrubs had significantly lower hydraulic safety margins than CK, with FD being significantly lower than CD. Notably, FD had lower carbon assimilation and a lower leaf non-structural carbon concentration, but higher stem non-structural carbon concentration. The results of a principal component analysis showed that net photosynthetic rate, sapwood specific conductivity, embolism resistance, midday water potential, and leaf and stem soluble sugar concentration were the main axes of variation for R. soongorica traits. CK had the highest water use efficiency, net photosynthetic rate, and gas exchange rate, while FD had the lowest embolism resistance and highest osmoregulation. Midday water potential, leaf and stem soluble sugar concentration were the main axes of variation for S. passerina traits, and individual distribution under three water treatments was associated with drought tolerance traits. The findings suggest that species exhibit different response strategies for resistance to drought stress, with R. soongorica being drought-avoidant and S. passerina being drought-tolerant. These findings highlight the adaptive capacity of desert shrubs to water deficit and provide insights for assessing hydraulic failure and carbon starvation in desert shrubs.
Optimizing the Solvent Selection of the Ultrasound-Assisted Extraction of Sea Buckthorn (Hippophae rhamnoides L.) Pomace: Phenolic Profiles and Antioxidant Activity
Authors: Wu Dan , Yang Zhihao , Li Jiong , Huang Huilin , Xia Qile , Ye Xingqian , Liu Donghong
Published: February 2024

Sea buckthorn pomace (SBP) is a by-product of sea buckthorn processing that is rich in bioactive compounds. In this study, different active ingredients were extracted by using different solvents (water, methanol, ethanol, glycerol, ethyl acetate, and petroleum ether) combined with an ultrasonic assisted method. The correlation between the active ingredients and antioxidant properties of the extract was studied, which provided a research basis for the comprehensive utilization of SBP. This study revealed that the 75% ethanol extract had the highest total phenolic content (TPC) of 42.86 ± 0.73 mg GAE/g, while the 75% glycerol extract had the highest total flavonoid content (TFC) of 25.52 ± 1.35 mg RTE/g. The ethanol extract exhibited the strongest antioxidant activity at the same concentration compared with other solvents. The antioxidant activity of the ethanol, methanol, and glycerol extracts increased in a concentration-dependent manner. Thirteen phenolic compounds were detected in the SBP extracts using UPLC-MS/MS analysis. Notably, the 75% glycerol extract contained the highest concentration of all identified phenolic compounds, with rutin (192.21 ± 8.19 μg/g), epigallocatechin (105.49 ± 0.69 μg/g), and protocatechuic acid (27.9 ± 2.38 μg/g) being the most abundant. Flavonols were found to be the main phenolic substances in SBP. A strong correlation was observed between TPC and the antioxidant activities of SBP extracts. In conclusion, the choice of solvent significantly influences the active compounds and antioxidant activities of SBP extracts. SBP extracts are a valuable source of natural phenolics and antioxidants.
An Updated Comprehensive Overview of Different Food Applications of W1/O/W2 and O1/W/O2 Double Emulsions
Authors: Ghiasi Fatemeh , Hashemi Hadi , Esteghlal Sara , Hosseini Seyed Mohammad Hashem
Published: February 2024

Double emulsions (DEs) present promising applications as alternatives to conventional emulsions in the pharmaceutical, cosmetic, and food industries. However, most review articles have focused on the formulation, preparation approaches, physical stability, and release profile of encapsulants based on DEs, particularly water-in-oil-in-water (W1/O/W2), with less attention paid to specific food applications. Therefore, this review offers updated detailed research advances in potential food applications of both W1/O/W2 and oil-in-water-in-oil (O1/W/O2) DEs over the past decade. To this end, various food-relevant applications of DEs in the fortification; preservation (antioxidant and antimicrobial targets); encapsulation of enzymes; delivery and protection of probiotics; color stability; the masking of unpleasant tastes and odors; the development of healthy foods with low levels of fat, sugar, and salt; and design of novel edible packaging are discussed and their functional properties and release characteristics during storage and digestion are highlighted.
Optimization, In Vitro and Ex Vivo Assessment of Nanotransferosome Gels Infused with a Methanolic Extract of Solanum xanthocarpum for the Topical Treatment of Psoriasis
Authors: Sahu Nilanchala , Alam Perwez , Ali Asad , Kumar Neeraj , Tyagi Rama , Madan Swati , Walia Ramanpreet , Saxena Shikha
Published: February 2024

The goal of this investigation is to improve the topical delivery of medicine by preparing and maximizing the potential of a nanotransferosome gel infused with Solanum xanthocarpum methanolic extract (SXE) to provide localized and regulated distribution. Thin-film hydration was used to create SXE-infused nanotransferosomes (SXE-NTFs), and a Box–Behnken design was used to improve them. Phospholipon 90G (X1), cholesterol (X2) and sodium cholate (X3) were chosen as the independent variables, and their effects on vesicle size (Y1), polydispersity index (PDI) (Y2) and the percentage of entrapment efficiency (EE) (Y3) were observed both individually and in combination. For the SXE-NTFs, the vesicle size was 146.3 nm, the PDI was 0.2594, the EE was 82.24 ± 2.64%, the drug-loading capacity was 8.367 ± 0.07% and the drug release rate was 78.86 ± 5.24%. Comparing the antioxidant activity to conventional ascorbic acid, it was determined to be 83.51 ± 3.27%. Ex vivo permeation tests revealed that the SXE-NTF gel (82.86 ± 2.38%) considerably outperformed the SXE gel (35.28 ± 1.62%) in terms of permeation. In addition, it seemed from the confocal laser scanning microscopy (CLSM) picture of the Wistar rat’s skin that the rhodamine-B-loaded SXE-NTF gel had a higher penetration capability than the control. Dermatokinetic studies showed that the SXE-NTF gel had a better retention capability than the SXE gel. According to the experimental results, the SXE-NTF gel is a promising and successful topical delivery formulation.
Gels in Motion: Recent Advancements in Energy Applications
Authors: Singh Aditya Narayan , Meena Abhishek , Nam Kyung-Wan
Published: February 2024

Gels are attracting materials for energy storage technologies. The strategic development of hydrogels with enhanced physicochemical properties, such as superior mechanical strength, flexibility, and charge transport capabilities, introduces novel prospects for advancing next-generation batteries, fuel cells, and supercapacitors. Through a refined comprehension of gelation chemistry, researchers have achieved notable progress in fabricating hydrogels endowed with stimuli-responsive, self-healing, and highly stretchable characteristics. This mini-review delineates the integration of hydrogels into batteries, fuel cells, and supercapacitors, showcasing compelling instances that underscore the versatility of hydrogels, including tailorable architectures, conductive nanostructures, 3D frameworks, and multifunctionalities. The ongoing application of creative and combinatorial approaches in functional hydrogel design is poised to yield materials with immense potential within the domain of energy storage.
Systems Are Overstretched from the COVID-19 Pandemic: An Interpretive Description of Disabled People’s Access to Healthcare and Disability Support in New Zealand
Authors: Nazari Orakani Solmaz , Officer Tara N. , Good Gretchen , McBride-Henry Karen
Published: February 2024

The COVID-19 pandemic disrupted healthcare and support services, creating challenges for disabled people. New Zealand implemented a range of policies to prevent and limit viral transmission of COVID-19. This study investigates disabled people’s experiences accessing healthcare and disability support services during the COVID-19 pandemic, and based on this analysis, the implications of public health policy decisions on disabled people’s experiences during the pandemic in New Zealand are explicated. A qualitative design underpinned by interpretive description methodology guided this study. A total of 64 disabled people or parents of disabled children participated in semi-structured interviews. The team of health services and disability researchers then engaged in an iterative thematic approach to analysis, which led to three key themes: (1) protective personal factors, which assisted disabled people to access healthcare and support services, (2) immediate pandemic policy impacts, including policy and legislative changes, which created additional access barriers for disabled people, and (3) exacerbating factors, including compounding vulnerabilities, overstretched systems, and the impact of the vaccine mandate, which worsened the already limited access to healthcare and disability services for disabled people. The pandemic overwhelmed an already stretched healthcare and disability support system, resulting in service disruptions with negative consequences for disabled people’s health and wellbeing. Future policy development needs to be disability-centred in its inclusion of people with lived experience and consideration of the support needs of disabled populations. A first step in this process could include pandemic planning and policy co-design to ensure a continuum of healthcare services and support availability for individuals when services are disrupted. In addition, access to formal and informal support for disabled people should be recognised as a fundamental human right when accessing healthcare and disability support services.
Hospital Incidence, Sex Disparities, and Perioperative Mortality in Open Surgically Treated Patients with Aneurysms of the Ascending Aorta and Aortic Arch in Switzerland
Authors: Menges Anna-Leonie , Zimmermann Alexander , Stoklasa Kerstin , Reitnauer Daniela , Meuli Lorenz , Reutersberg Benedikt
Published: February 2024

Objective. To analyze the epidemiological shifts in the incidence of ascending and arch aortic aneurysms (AA) treated with open surgery in the context of evolving endovascular options on a national basis. Methods. Between 1 January 2009 and 31 December 2018, 4388 cases were admitted to the hospital with either ruptured (r)AA or non-ruptured (nr)AA as the primary or secondary diagnosis. Patients were classified as having AA based on inclusion and exclusion criteria. Results. The age-standardized hospital incidence rates for treatment of nrAA were 7.8 (95% confidence interval (CI): 6.9 to 8.7) in 100,000 men and 2.9 (2.4 to 3.4) in 100,000 women and were stable over time. The overall raw in-hospital mortality rate was 2.0% and was significantly lower in males compared to women (1.6% vs. 2.8%, p = 0.015). Higher van Walraven scores (OR: 1.08 per point; 95%CI: 1.06 to 1.11; p = 0.001) and higher age (OR 1.05 per year; (95%CI: 1.02 to 1.07, p = 0.045) were significantly associated with hospital mortality. Conclusions. Endovascular surgery seems to have no influence on hospital incidence in patients treated with conventional surgery for AA in Switzerland. There was a significant reduction in in-hospital mortality in both men and women, with age and the von Walraven score being independent factors for worse outcomes.
Oral Health Status and Oral Health-Related Behaviours of Hong Kong Students with Vision Impairment
Authors: Lee Jessica Ka Yi , Yuen Agatha Wing Tung , Leung Karen Pui Yan , Li Joyce Tin Wing , Bae Seon Yeong , Chan Yi Yung , Ip Ching Kiu , Lau Sik Hong , Lau Yin Ngai , Lo Hei Yuet , Tang Shuk Kwan , Duangthip Duangporn
Published: February 2024

This cross-sectional epidemiological study aimed to describe dental caries and oral hygiene conditions among visually impaired students in Hong Kong. Students aged 6–21 years from two specialised schools for those with vision impairment were invited. Information on sociodemographic background and oral health-related behaviours was collected through an online parent-reported questionnaire. Dental caries and oral hygiene were assessed using the Decayed, Missing and Filled Teeth (DMFT) index and the Visible Plaque Index (VPI), respectively. Chi-square, Mann–Whitney U and Kruskal–Wallis H tests were conducted to analyse the association between students’ background and oral health status. A total of 73 participants were recruited, of whom 57.5% were male. Their mean (SD) age was 12.9 (4.7) years. Their mean DMFT score (SD) was 1.0 (1.8), and 43.8% had caries experience. The mean VPI (SD) was 0.76 (0.30). Their caries experience was significantly associated with their snacking habits (p = 0.013). Male participants had poorer oral hygiene than females (p = 0.048). In summary, dental caries is prevalent among visually impaired students in Hong Kong and their oral hygiene condition is unsatisfactory. Caries experience is significantly associated with snacking frequency, whereas oral hygiene is associated with gender. More specially designed preventive oral health measures should be provided for visually impaired students and their caretakers.
The Inhibition of Serine Proteases by Serpins Is Augmented by Negatively Charged Heparin: A Concise Review of Some Clinically Relevant Interactions
Authors: Chan Edward D. , King Paul T. , Bai Xiyuan , Schoffstall Allen M. , Sandhaus Robert A. , Buckle Ashley M.
Published: February 2024

Serine proteases are members of a large family of hydrolytic enzymes in which a particular serine residue in the active site performs an essential role as a nucleophile, which is required for their proteolytic cleavage function. The array of functions performed by serine proteases is vast and includes, among others, the following: (i) the ability to fight infections; (ii) the activation of blood coagulation or blood clot lysis systems; (iii) the activation of digestive enzymes; and (iv) reproduction. Serine protease activity is highly regulated by multiple families of protease inhibitors, known collectively as the SERine Protease INhibitor (SERPIN). The serpins use a conformational change mechanism to inhibit proteases in an irreversible way. The unusual conformational change required for serpin function provides an elegant opportunity for allosteric regulation by the binding of cofactors, of which the most well-studied is heparin. The goal of this review is to discuss some of the clinically relevant serine protease–serpin interactions that may be enhanced by heparin or other negatively charged polysaccharides. The paired serine protease–serpin in the framework of heparin that we review includes the following: thrombin–antithrombin III, plasmin–anti-plasmin, C1 esterase/kallikrein–C1 esterase inhibitor, and furin/TMPRSS2 (serine protease Transmembrane Protease 2)–alpha-1-antitrypsin, with the latter in the context of COVID-19 and prostate cancer.
Genetic Factors Associated with the Development of Neuropathy in Type 2 Diabetes
Authors: Tordai Dóra Zsuszanna , Hajdú Noémi , Rácz Ramóna , Istenes Ildikó , Békeffy Magdolna , Vági Orsolya Erzsébet , Kempler Miklós , Körei Anna Erzsébet , Tóbiás Bálint , Illés Anett , Pikó Henriett , Kósa János Pál , Árvai Kristóf , Papp Márton , Lakatos Péter András , Kempler Péter , Putz Zsuzsanna
Published: February 2024

Neuropathy is a serious and frequent complication of type 2 diabetes (T2DM). This study was carried out to search for genetic factors associated with the development of diabetic neuropathy by whole exome sequencing. For this study, 24 patients with long-term type 2 diabetes with neuropathy and 24 without underwent detailed neurological assessment and whole exome sequencing. Cardiovascular autonomic function was evaluated by cardiovascular reflex tests. Heart rate variability was measured by the triangle index. Sensory nerve function was estimated by Neurometer and Medoc devices. Neuropathic symptoms were characterized by the neuropathy total symptom score (NTSS). Whole exome sequencing (WES) was performed on a Thermo Ion GeneStudio S5 system determining the coding sequences of approximately 32,000 genes comprising 50 million base pairs. Variants were detected by Ion Reporter software and annotated using ANNOVAR, integrating database information from dbSNP, ClinVar, gnomAD, and OMIM. Integrative genomics viewer (IGV) was used for visualization of the mapped reads. We have identified genetic variants that were significantly associated with increased (22–49-fold) risk of neuropathy (rs2032930 and rs2032931 of recQ-mediated genome instability protein 2 (RMI2) gene), rs604349 of myosin binding protein H like (MYBPHL) gene and with reduced (0.07–0.08-fold) risk (rs917778 of multivesicular body subunit 12B (MVB12B) and rs2234753 of retinoic acid X receptor alpha (RXRA) genes). The rs2032930 showed a significant correlation with current perception thresholds measured at 5 Hz and 250 Hz for n. medianus (p = 0.042 and p = 0.003, respectively) and at 5 Hz for n. peroneus (p = 0.037), as well as the deep breath test (p = 0.022) and the NTSS (p = 0.023). The rs2032931 was associated with current perception thresholds (p = 0.003 and p = 0.037, respectively), deep breath test (p = 0.022), and NTSS (p = 0.023). The rs604349 correlated with values measured at 2000 (p = 0.049), 250 (p = 0.018), and 5 Hz (p = 0.005) for n. medianus, as well as warm perception threshold measured by Medoc device (p = 0.042). The rs2234753 showed correlations with a current perception threshold measured at 2000 Hz for n. medianus (p = 0.020), deep breath test (p = 0.040), and NTSS (p = 0.003). There was a significant relationship between rs91778 and cold perception threshold (p = 0.013). In our study, genetic variants have been identified that may have an impact on the risk of neuropathy developing in type 2 diabetic patients. These results could open up new opportunities for early preventive measures and might provide targets for new drug developments in the future.
Mitogenomic Characterization and Phylogenetic Placement of African Hind, Cephalopholis taeniops: Shedding Light on the Evolution of Groupers (Serranidae: Epinephelinae)
Authors: Kundu Shantanu , Kang Hye-Eun , Kim Ah Ran , Lee Soo Rin , Kim Eun-Bi , Amin Muhammad Hilman Fu’adil , Andriyono Sapto , Kim Hyun-Woo , Kang Kyoungmi
Published: February 2024

The global exploration of evolutionary trends in groupers, based on mitogenomes, is currently underway. This research extensively investigates the structure of and variations in Cephalopholis species mitogenomes, along with their phylogenetic relationships, focusing specifically on Cephalopholis taeniops from the Eastern Atlantic Ocean. The generated mitogenome spans 16,572 base pairs and exhibits a gene order analogous to that of the ancestral teleost’s, featuring 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich control region. The mitogenome of C. taeniops displays an AT bias (54.99%), aligning with related species. The majority of PCGs in the mitogenome initiate with the start codon ATG, with the exceptions being COI (GTG) and atp6 (TTG). The relative synonymous codon usage analysis revealed the maximum abundance of leucine, proline, serine, and threonine. The nonsynonymous/synonymous ratios were <1, which indicates a strong negative selection among all PCGs of the Cephalopholis species. In C. taeniops, the prevalent transfer RNAs display conventional cloverleaf secondary structures, except for tRNA-serine (GCT), which lacks a dihydrouracil (DHU) stem. A comparative examination of conserved domains and sequence blocks across various Cephalopholis species indicates noteworthy variations in length and nucleotide diversity. Maximum likelihood, neighbor-joining, and Bayesian phylogenetic analyses, employing the concatenated PCGs and a combination of PCGs + rRNAs, distinctly separate all Cephalopholis species, including C. taeniops. Overall, these findings deepen our understanding of evolutionary relationships among serranid groupers, emphasizing the significance of structural considerations in mitogenomic analyses.
The Role of BmTMED6 in Female Reproduction in Silkworm, Bombyx mori
Authors: Wang Chunyang , Hu Zunmei , Guo Yu , Xiao Wenfu , Zhang Youhong , Zhou Anlian , Chen Ping
Published: February 2024

Transmembrane emp24 domain (TMED) proteins have been extensively studied in mammalian embryonic development, immune regulation, and signal transduction. However, their role in insects, apart from Drosophila melanogaster, remains largely unexplored. Our previous study demonstrated the abundant expression of BmTMED6 across all stages and tissues of the silkworm. In this study, we investigate the function of BmTMED6 in reproduction. We observe significant differences in the expression of BmTMED6 between male and female silkworms, particularly in the head and fatboby, during the larval stage. Furthermore, qRT-PCR and WB analysis reveal substantial variation in BmTMED6 levels in the ovaries during pupal development, suggesting a potential association with silkworm female reproduction. We find that reducing TMED6 expression significantly decreases the number of eggs laid by female moths, leading to an accumulation of unlaid eggs in the abdomen. Moreover, downregulation of BmTMED6 leads to a decrease in the expression of BmDop2R1 and BmDop2R2, while overexpression of BmTMED6 in vitro has the opposite effect. These indicate that BmTMED6 plays a role in oviposition in female moths, potentially through the dopamine signaling pathway. This study provides a new regulatory mechanism for female reproduction in insects.
Relationship between the Pre-ECMO and ECMO Time and Survival of Severe COVID-19 Patients: A Systematic Review and Meta-Analysis
Authors: Tan Ziqi , Su Longxiang , Chen Xiangyu , He Huaiwu , Long Yun
Published: February 2024

Background: Coronavirus disease 2019 (COVID-19) is the etiology of acute respiratory distress syndrome (ARDS). Extracorporeal membrane oxygenation (ECMO) is used to support gas exchange in patients who have failed conventional mechanical ventilation. However, there is no clear consensus on the timing of ECMO use in severe COVID-19 patients. Objective: The aim of this study is to compare the differences in pre-ECMO time and ECMO duration between COVID-19 survivors and non-survivors and to explore the association between them. Methods: PubMed, the Cochrane Library, Embase, and other sources were searched until 21 October 2022. Studies reporting the relationship between ECMO-related time and COVID-19 survival were included. All available data were pooled using random-effects methods. Linear regression analysis was used to determine the correlation between pre-ECMO time and ECMO duration. The meta-analysis was registered with PROSPERO under registration number CRD42023403236. Results: Out of the initial 2473 citations, we analyzed 318 full-text articles, and 54 studies were included, involving 13,691 patients. There were significant differences between survivors and non-survivors in the time from COVID-19 diagnosis (standardized mean difference (SMD) = −0.41, 95% confidence interval (CI): [−0.53, −0.29], p < 0.00001), hospital (SMD = −0.53, 95% CI: [−0.97, −0.09], p = 0.02) and intensive care unit (ICU) admission (SMD = −0.28, 95% CI: [−0.49, −0.08], p = 0.007), intubation or mechanical ventilation to ECMO (SMD = −0.21, 95% CI: [−0.32, −0.09], p = 0.0003) and ECMO duration (SMD = −0.18, 95% CI: [−0.30, −0.06], p = 0.003). There was no statistical association between a longer time from symptom onset to ECMO (hazard ratio (HR) = 1.05, 95% CI: [0.99, 1.12], p = 0.11) or time from intubation or mechanical ventilation (MV) and the risk of mortality (highest vs. lowest time groups odds ratio (OR) = 1.18, 95% CI: [0.78, 1.78], p = 0.42; per one-day increase OR = 1.14, 95% CI: [0.86, 1.52], p = 0.36; HR = 0.99, 95% CI: [0.95, 1.02], p = 0.39). There was no linear relationship between pre-ECMO time and ECMO duration. Conclusion: There are differences in pre-ECMO time between COVID-19 survivors and non-survivors, and there is insufficient evidence to conclude that longer pre-ECMO time is responsible for reduced survival in COVID-19 patients. ECMO duration differed between survivors and non-survivors, and the timing of pre-ECMO does not have an impact on ECMO duration. Further studies are needed to explore the association between pre-ECMO and ECMO time in the survival of COVID-19 patients.
Dupilumab for Treatment of Prurigo Nodularis: Real-Life Effectiveness for up to 84 Weeks
Authors: Paganini Claudia , Talamonti Marina , Maffei Virginia , Di Raimondo Cosimo , Bianchi Luca , Galluzzo Marco
Published: February 2024

(1) Background: Prurigo nodularis (PN) is a persistent and inflammatory dermatological condition characterized by chronic itching and the formation of hardened nodules, significantly impacting the affected individuals’ quality of life and psychological well-being. The management of PN poses challenges due to the limited efficacy and undesirable side effects associated with current interventions. (2) Methods: This article examines sixteen patients affected by PN treated with dupilumab, a fully human monoclonal antibody targeting interleukin IL-4 and IL-13 signaling. This involves a retrospective descriptive statistical analysis. (3) Results and (4) Conclusions: In all patients, dupilumab proves to be an effective drug in achieving disease clearance, as indicated by all the parameters considered as assessed by both physicians and patients at each evaluation point (Week 6, Week 16, Week 32, Week 52, Week 68, and Week 84), in comparison to the initial baseline.
Steroids and/or Non-Steroidal Anti-Inflammatory Drugs as Postoperative Treatment after Trabeculectomy—12-Month Results of a Randomized Controlled Trial
Authors: Ahmadzadeh Afrouz , Kessel Line , Schmidt Bo Simmendefeldt , Kolko Miriam , Bach-Holm Daniella
Published: February 2024

This prospective randomized controlled trial aimed to compare the efficacy and safety of topical preservative-free diclofenac (DICLO) to dexamethasone (DEX) eyedrops, and their combination (DEX+DICLO) after trabeculectomy. Sixty-nine patients with medically uncontrolled glaucoma were randomized to receive topical postoperative treatment with DICLO (n = 23), DEX (n = 23), or a combination of DEX and DICLO (n = 23). The primary outcome was the intraocular pressure (IOP) 12 months postoperatively. Secondary outcomes included surgical success, failure, visual field, and visual acuity from baseline to 12 months postoperatively. IOP reached the lowest point one day after trabeculectomy. At 12 months, IOP was 10.0 mmHg (95% CI, 8.4–11.6 mmHg) for DICLO, 10.9 mmHg (95% CI, 9.4–12.3 mmHg) for DEX, and 11.2 mmHg (95% CI, 9.1–13.3 mmHg) for DEX+DICLO. There were no significant differences in IOP, surgical success, failure, visual field, or visual acuity between the DICLO, DEX, or DEX+DICLO groups. We found that topical diclofenac was not statistically different from topical dexamethasone in controlling IOP 12 months after trabeculectomy. Combining diclofenac and dexamethasone offered no added IOP control compared to dexamethasone alone.
Supplementary Respiratory Therapy Improves Pulmonary Function in Pediatric Patients with Cerebral Palsy: A Systematic Review and Meta-Analysis
Authors: Kolumbán Erika , Szabados Márton , Hernádfői Márk , Nguyen Do To Uyen , Nagy Rita , Zolcsák Ádám , Müller Katalin Eszter , Sipos Zoltán , Veres Dániel Sándor , Szőllősi Anett , Hegyi Péter , Garami Miklós , Túri Ibolya
Published: February 2024

Background: Despite medical advances, individuals with cerebral palsy (CP) face significant respiratory challenges, leading to heightened hospitalization rates and early mortality among this population. We hypothesize that integrating supplementary respiratory therapy into standard rehabilitation will result in significant improvements in pulmonary function, enhanced respiratory muscle strength, and an overall increase in the quality of life among pediatric patients with CP. Methods: A systematic search of literature across five databases was conducted, and random-effects meta-analyses were performed to assess the impact of supplementary respiratory therapy on (a) pulmonary function: forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), FVC/FEV1 ratio, peak expiratory flow (PEF), and (b) respiratory muscle strength: maximal inspiratory and expiratory pressure (MIP, MEP), and (c) quality of life. Certainty of evidence was determined by the GRADE assessment. Results: Analysis of data from 11 eligible randomized controlled trials revealed clinically meaningful changes in pulmonary function. We found a relevant mean difference (MD) in absolute PEF of 0.50 L/s (95% confidence interval (CI): 0.19; 0.82 p = 0.0107). The certainty of the evidence ranged from moderate to high. Conclusions: This study presents current evidence on the impact of various supplementary respiratory therapies for CP patients classified under gross motor function classification level I–IV, demonstrating clinically meaningful improvements in pulmonary function and respiratory muscle strength. These improvements suggest the potential for an enhanced quality of life. Our findings hold the promise of serving as a foundational reference for potential revisions to conventional rehabilitation care, incorporating supplementary respiratory therapy.
An Anti-Oxidative Bioink for Cartilage Tissue Engineering Applications
Authors: Chen Xin , Yang Mengni , Zhou Zheng , Sun Jingjing , Meng Xiaolin , Huang Yuting , Zhu Wenxiang , Zhu Shuai , He Ning , Zhu Xiaolong , Han Xiaoxiao , Liu Hairong
Published: February 2024

Since chondrocytes are highly vulnerable to oxidative stress, an anti-oxidative bioink combined with 3D bioprinting may facilitate its applications in cartilage tissue engineering. We developed an anti-oxidative bioink with methacrylate-modified rutin (RTMA) as an additional bioactive component and glycidyl methacrylate silk fibroin as a biomaterial component. Bioink containing 0% RTMA was used as the control sample. Compared with hydrogel samples produced with the control bioink, solidified anti-oxidative bioinks displayed a similar porous microstructure, which is suitable for cell adhesion and migration, and the transportation of nutrients and wastes. Among photo-cured samples prepared with anti-oxidative bioinks and the control bioink, the sample containing 1 mg/mL of RTMA (RTMA-1) showed good degradation, promising mechanical properties, and the best cytocompatibility, and it was selected for further investigation. Based on the results of 3D bioprinting tests, the RTMA-1 bioink exhibited good printability and high shape fidelity. The results demonstrated that RTMA-1 reduced intracellular oxidative stress in encapsulated chondrocytes under H2O2 stimulation, which results from upregulation of COLII and AGG and downregulation of MMP13 and MMP1. By using in vitro and in vivo tests, our data suggest that the RTMA-1 bioink significantly enhanced the regeneration and maturation of cartilage tissue compared to the control bioink, indicating that this anti-oxidative bioink can be used for 3D bioprinting and cartilage tissue engineering applications in the future.
Image Inpainting Forgery Detection: A Review
Authors: Barglazan Adrian-Alin , Brad Remus , Constantinescu Constantin
Published: February 2024

In recent years, significant advancements in the field of machine learning have influenced the domain of image restoration. While these technological advancements present prospects for improving the quality of images, they also present difficulties, particularly the proliferation of manipulated or counterfeit multimedia information on the internet. The objective of this paper is to provide a comprehensive review of existing inpainting algorithms and forgery detections, with a specific emphasis on techniques that are designed for the purpose of removing objects from digital images. In this study, we will examine various techniques encompassing conventional texture synthesis methods as well as those based on neural networks. Furthermore, we will present the artifacts frequently introduced by the inpainting procedure and assess the state-of-the-art technology for detecting such modifications. Lastly, we shall look at the available datasets and how the methods compare with each other. Having covered all the above, the outcome of this study is to provide a comprehensive perspective on the abilities and constraints of detecting object removal via the inpainting procedure in images.
Carbon and Nitrogen Stocks in Agricultural Soils under Different Natural Conditions and Management in Slovakia
Authors: Kizeková Miriam , Kanianska Radoslava , Jančová Ľubica , Čunderlík Jozef , Dugátová Zuzana
Published: February 2024

Soil is a natural capital which supplies valuable ecosystem services including carbon and nitrogen storage. Agroecosystems play an important role in soil organic carbon (SOC) and soil total nitrogen (NT) accumulation. The aim of this study was to analyse SOC stock (SOCS) and NT stock (NTS) in relation to land use (arable land-AL, permanent grasslands-PG), management, soil depth, and selected soil properties of six soil subtypes (Rendzic Leptosol—LPrz, Dystric Cambisol—CMdy, Stagnic Cambisol—CMst, Haplic Fluvisol—FLha, Gleyic Fluvisol—FLgl, Haplic Chernozem—CHha) which are the most widespread in Slovakia. SOCS for a 50 cm deep soil profile ranged from 161 t.ha−1 in CHha to 59 t.ha−1 in FLgl in grasslands, and in arable lands from 111 t.ha−1 in CHha to 38 t.ha−1 in CMst. In grasslands, FLs and CMst showed the significantly lowest SOCS and NTS in comparison to CMdy, LPrz, and CHha. The mean soil NT content in arable land and grasslands was 2.21 g.kg−1 and 2.82 g.kg−1, respectively. ANOVA showed that soil subtype, land use, and site have significantly affected SOCS but not NTS. The correlation analysis revealed correlations between SOCS and NTS. SOCS was also correlated with C:N, pH, P, and K. This study should help to encourage practices to maintain soil C and soil properties and to ensure the sustainability of the functions of many soil types in Slovakia.
Isolation and Characterization of Mycoplasma ovipneumoniae Infecting Goats with Pneumonia in Anhui Province, China
Authors: Chen Jiahong , Wang Shijia , Dong Dong , Zhang Zijun , Huang Yafeng , Zhang Yong
Published: February 2024

Mycoplasma ovipneumoniae (M. ovipneumoniae) causes a fatal infection in goats, leading to significant economic losses in the small-ruminant industry worldwide. The present study aimed to characterize the strains of M. ovipneumoniae infecting goats with pneumonia in Anhui Province, China. From November 2021 to January 2023, among 20 flocks, a total of 1320 samples (600 samples of unvaccinated blood, 400 nasal swabs, 200 samples of pleural fluid, and 120 samples of lung tissue) were obtained from goats with typical signs of pneumonia, such as a low growth rate, appetite suppression, increased temperature, discharge from the nose, and a cough. Necropsied goats showed increased pleural fluid, fibrinous pleuropneumonia, and attached localized pleural adhesions. M. ovipneumoniae isolated from the samples were subjected to an indirect hemagglutination test (IHA), PCR amplicon sequencing, phylogenetic analysis, and biochemical identification tests. The overall positivity rate of M. ovipneumoniae was 27.50%. Mycoplasmas were obtained from 80 (20.0%) nasal swabs, 21 (10.5%) pleural fluid samples, and 15 (12.5%) lung samples. PCR amplicon (288 bp) sequencing identified eight strains of M. ovipneumoniae. In a phylogenetic tree, the isolated strains were homologous to the standard strain M. ovipneumoniae Y-98 and most similar to M. ovipneumoniae FJ-SM. Local strains of M. ovipneumoniae were isolated from goats in Anhui province. The identified genomic features and population structure will promote further study of M. ovipneumoniae pathogenesis and could form the basis for vaccine and therapy development.
(E)-5-[Bromo(phenyl)methylene]-4-phenyl-2-(p-tolyl)-4,5-dihydrooxazole
Authors: Morita Nobuyoshi , Kurami Saki , Ishii Naho , Tanaka Kosaku , Hashimoto Yoshimitsu , Tamura Osamu
Published: February 2024

One-pot synthesis of (E)-5-[bromo(phenyl)methylene]-4-phenyl-2-(p-tolyl)-4,5-dihydrooxazole (9) from propargylic alcohol 5 and p-toluamide (6) was achieved via gold(III)-catalyzed propargylic substitution, followed by gold(III)-catalyzed bromocyclization. The structure of 9 was confirmed by an X-ray crystallographic analysis.
High-Entropy Diborides—Silicon Carbide Composites by Reactive and Non-Reactive Spark Plasma Sintering: A Comparative Study
Authors: Pakhomova Ekaterina , Cao Giacomo , Orrù Roberto , Garroni Sebastiano , Ferro Paolo , Licheri Roberta
Published: February 2024

The reactive spark plasma sintering (R-SPS) method was compared in this work with the two-step SHS–SPS route, based on the combination of the self-propagating high-temperature synthesis (SHS) with the SPS process, for the fabrication of dense (Hf0.2Mo0.2Ti0.2Ta0.2Nb0.2)B2–SiC and (Hf0.2Mo0.2Ti0.2Ta0.2Zr0.2)B2–SiC ceramics. A multiphase and inhomogeneous product, containing various borides, was obtained at 2000 °C/20 min by R-SPS from transition metals, B4C, and Si. In contrast, if the same precursors were first reacted by SHS and then processed by SPS under the optimized condition of 1800 °C/20 min, the desired ceramics were successfully attained. The resulting sintered samples possessed relative densities above 97% and displayed uniform microstructures with residual oxide content <2.4 wt.%. The presence of SiC made the sintering temperature milder, i.e., 150 °C below that needed by the corresponding additive-free system. The fracture toughness was also markedly improved, particularly when considering the Nb-containing system processed at 1800 °C/20 min, whereas the fracture toughness progressively decreased (from 7.35 to 5.36 MPa m1/2) as the SPS conditions became more severe. SiC addition was found to inhibit the volatilization of metal oxides like MoO3 formed during oxidation experiments, thus avoiding mass loss in the ceramics. The benefits above also likely took advantage of the fact that the two composite constituents were synthesized in parallel, according to the SHS–SPS approach, rather than being produced separately and combined subsequently, so that strong interfaces between them were formed.