bluuebunny/crossref_metadata_2025 huggingface dataset:
Strategi Perusahaan Kosmetik Korea Untuk Memperkuat Marketing Di Indonesia
Authors: Lee Jweyoung , Premananto Gancar Candra
Published: November 2023

Assessment of beauty in other countries is very different, this shows that beauty is something that is very relative and cannot be measured. Several factors cause the construction of beauty in each area to be different, one of which is the cultural factor. It is undeniable, in the current era of globalization, industrial development is growing rapidly in countries around the world. Beauty is very important for Korean society, especially women. This is the basis why cosmetics are the primary needs of society and the demand for cosmetics in the market continues to increase from year to year. In the case of Korea, the number of Muslim tourists is increasing year by year, and their Korean cosmetic purchases are also increasing rapidly. In this study, the author uses a qualitative method by means of structured interviews regarding the use and interest in cosmetic products originating  from Korea by explaining several reference questions, including:  This  study  tries  to  suggest  a  more  successful  entry  strategy  for  Korean cosmetic companies in the Indonesian cosmetic market. However, although a successful entry strategy in this study can explain the Indonesian cosmetic market, it is difficult to explain it as a successful entry strategy into other Southeast Asian countries or Islamic cultural markets.

Keywords: Cosmetics; Marketing; Strategy
LIFE: A metric for quantitively mapping the impact of land-cover change on global extinctions
Authors: Eyres Alison , Ball Thomas , Dales Michael , Swinfield Tom , Arnell Andy , Baisero Daniele , Durán América Paz , Green Jonathan , Madhavapeddy Anil , Balmford Andrew
Published: November 2023

Tracking and preventing continued extinctions requires robust spatially explicit metrics quantifying the impacts of human activities on habitat extent. Existing metrics either fail to consider species identity or focus solely on recent habitat losses. The persistence score approach represented an important development by combining species ecologies and land-cover data whilst considering the cumulative and non-linear impact of past habitat loss on species’ probability of persistence. However, it is computationally demanding, limiting its global use and application. Here we couple the persistence score approach with high-performance computing to generate global maps of what we term the LIFE (Land-cover change Impacts on Future Extinctions) metric for 29153 species of terrestrial vertebrates at 1 arc-minute resolution (3.4km2 at the equator). These maps quantify, for the first time, the marginal changes in the expected number of extinctions caused by (1) converting remaining natural vegetation to agriculture, and (2) restoring farmland to natural habitat. We demonstrate statistically that this approach integrates information on species richness, endemism, and past habitat loss. Our resulting maps can reliably be used at scales from 0.5-1000km2, and offer unprecedented opportunities to estimate the impact on extinctions of diverse actions, from dietary choices right through to global protected area development.
An amphibian toxin phenotype is sexually dimorphic and shows seasonal concordant change between sexes
Authors: Frey Anna R. , Bucciarelli Gary M. , Hu Daniel D. , Kats Lee B. , Green David B.
Published: November 2023

Sexually dimorphic phenotypes are consequential to animal survival, and this is especially apparent with defense phenotypes. Amphibians have poison glands, and several lineages maintain a neurotoxin, tetrodotoxin (TTX), which is largely considered a derived chemical defense trait. However, production pathways are unclear, and, as such, whether males and females differentially produce or appropriate toxin concentrations is not known. We evaluated the relationship between TTX concentrations, sex, and morphology by collecting trait data from more than 850 wild newts of the genus Taricha and tested for sex-based differences, potential sex-based changes to the phenotype between breeding seasons, and factors influencing toxicity. Females, regardless of reproductive status, exhibited greater concentrations than males, and temporal patterns indicate male and female trait values tend to fluctuate concordantly at short time scales, with females showing greater change over time. Tree regression results underscored the importance of size and sex to the phenotype. Sexual dimorphism and plasticity of toxins broaden the ecological roles of animal poisons beyond defense only and should recast how we model the evolution of tetrodotoxin.
Automated tooth crown design with optimized shape and biomechanics properties
Authors: Jin Xiaoxian , Wang Shengfa , Hu Jiangbei , Xu Xiaowei , Shi Yongji , Yu Haishi , Wang Jinwu , Li Kang , Cheng Xiaomin , Shao Moyu , Wang Hongkai
Published: November 2023

Despite the large demand for dental restoration each year, the design of crown restorations is mainly performed via manual software operation, which is tedious and subjective. Moreover, the current design process lacks biomechanics optimization, leading to localized stress concentration and reduced working life. To tackle these challenges, we develop a fully automated algorithm for crown restoration based on deformable model fitting and biomechanical optimization. From a library of dental oral scans, a conditional shape model (CSM) is constructed to represent the inter-teeth shape correlation. By matching the CSM to the patient’s oral scan, the optimal crown shape is estimated to coincide with the surrounding teeth. Next, the crown is seamlessly integrated into the finish line of preparation via a surface warping step. Finally, porous internal supporting structures of the crown are generated to avoid excessive localized stresses. This algorithm is validated on clinical oral scan data and achieved less than 2 mm mean surface distance as compared to the manual designs of experienced human operators. The mechanical simulation was conducted to prove that the internal supporting structures lead to uniform stress distribution all over the model.
Label-free spatially maintained measurements of metabolic phenotypes in cells
Authors: Hu Linghao , Wang Nianchao , Bryant Joshua D. , Liu Lin , Xie Linglin , West A. Phillip , Walsh Alex J.
Published: November 2023

Metabolic reprogramming at a cellular level contributes to many diseases including cancer, yet few assays are capable of measuring metabolic pathway usage by individual cells within living samples. Here, autofluorescence lifetime imaging is combined with single-cell segmentation and machine-learning models to predict the metabolic pathway usage of cancer cells. The metabolic activities of MCF7 breast cancer cells and HepG2 liver cancer cells were controlled by growing the cells in culture media with specific substrates and metabolic inhibitors. Fluorescence lifetime images of two endogenous metabolic coenzymes, reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD), were acquired by a multi-photon fluorescence lifetime microscope and analyzed at the cellular level. Quantitative changes of NADH and FAD lifetime components were observed for cells using glycolysis, oxidative phosphorylation, and glutaminolysis. Conventional machine learning models trained with the autofluorescence features classified cells as dependent on glycolytic or oxidative metabolism with 90%–92% accuracy. Furthermore, adapting convolutional neural networks to predict cancer cell metabolic perturbations from the autofluorescence lifetime images provided improved performance, 95% accuracy, over traditional models trained via extracted features. Additionally, the model trained with the lifetime features of cancer cells could be transferred to autofluorescence lifetime images of T cells, with a prediction that 80% of activated T cells were glycolytic, and 97% of quiescent T cells were oxidative. In summary, autofluorescence lifetime imaging combined with machine learning models can detect metabolic perturbations between glycolysis and oxidative metabolism of living samples at a cellular level, providing a label-free technology to study cellular metabolism and metabolic heterogeneity.
Rationale of construction processes in the social configuration of habitat: Nueva Colonia, Turbo, Colombia
Authors: Cuervo Calle Juan José , Salazar Hernández Cesar Augusto , Sánchez Holguín Victoria Eugenia , Lalinde Castrillón Luis Felipe , Sañudo Vélez Luis Guillermo
Published: November 2023

The alliance between universities, the private sector, the State and local communities becomes a fundamental strategy to channel regional development processes. This approach that interprets SDG 17 has been applied in the research courses of the Architecture Faculty of Universidad Pontificia Bolivariana. In these courses, real problems in specific regions of the national territory serve as a starting point to contribute to the training of the architect from the perspective of social responsibility. Specifically, this text focuses on the Sustainable Environment research course, which took as a case study the corregimiento of Nueva Colonia (Turbo- Colombia) to propose technologies that reconfigure positively urban and rural areas. These technologies are proposed as a response to the social, cultural, and economic realities and the conditions that govern construction activity in the region. Based on participatory design as a working method, students design tools that allow collecting information about the perception that the community has of their territory with the purpose of determining basic design guidelines defined collaboratively to provide solutions to the habitat and the built environment. These guidelines derive mainly from a reflection on technology in the construction of social housing, socio-environmental relationships, and their repercussions for the sustainable configuration of the built environment. To achieve this goal, students research on eco-efficient materials and local waste that can be used as construction material or raw material, and which are preliminarily evaluated using circularity and life cycle criteria. In this sense, this text seeks to highlight specific results of eco-efficient materials and waste from the banana industry.
Antibiotic resistance of Helicobacter pylori in Nanjing, China: a cross-section study from 2018 to 2023
Authors: Wei Wenjuan , Wang Zhibing , Li Chao , Jiang Zongdan , Zhang Zhenyu , Wang Shukui
Published: November 2023

Background

The increasing prevalence of antibiotic resistance in cases of Helicobacter pylori (H. pylori) infection has emerged as a significant global issue. This study offers a comprehensive examination of the alterations in drug resistance exhibited by H. pylori in the Nanjing region of China during the preceding five years. Another important objective is to investigate the influence of levofloxacin medication history on genotypic and phenotypic resistance.

Methods

This research screened 4277 individuals diagnosed with H. pylori infection between April 2018 and May 2023. The phenotype and genotypic resistance were evaluated using the Kirby-Bauer disk diffusion and PCR method.

Results

The most recent primary resistance rates for metronidazole, clarithromycin, levofloxacin, amoxicillin, furazolidone, and tetracycline were recorded at 77.23% (2385/3088), 37.24% (1150/3088), 27.72% (856/3088), 0.52% (16/3088), 0.19% (6/3088), and 0.06% (2/3088), respectively. For the recent five years, we observed a notable upsurge in the rate of metronidazole resistance and a slight elevation of clarithromycin and levofloxacin resistance. The documented resistance rates to single-drug, dual-drug, triple-drug, and quadruple-drug regimens were 35.39%, 28.32%, 25.72%, and 0.21%, respectively. The prevalence of multidrug-resistant strains escalated, rising from 37.96% in 2018 to 66.22% in 2023. The rate of phenotypic and genotypic resistance rate (57.10% and 65.57%) observed in strains obtained from patients without a levofloxacin treatment history was significantly lower than the rate in strains obtained from those with a history of levofloxacin treatment (88.73% and 94.74%). The prevailing gyrA mutations were primarily N87K (52.35%, 345/659), accompanied by D91N (13.96%, 92/659), and closely followed by D87G (10.77%, 71/659). For gyrA mutations, the 91-amino acid mutants exhibit a higher likelihood of discrepancies between phenotypic and genotypic resistance than the 87-amino acid mutants.

Conclusion

The extent of antibiotic resistance within H. pylori remains substantial within the Nanjing region. If levofloxacin proves ineffective in eradicating H. pylori during the initial treatment, its use in subsequent treatments is discouraged. The employment of levofloxacin resistance genotype testing can partially substitute conventional antibiotic sensitivity testing. Notably, predicting phenotypic resistance of levofloxacin through PCR requires more attention to the mutation type of gyrA to improve prediction accuracy.
Exploring the antibacterial and dermatitis-mitigating properties of chicken egg white-synthesized zinc oxide nano whiskers
Authors: Abdikakharovich Sidikov Akmal , Rauf Mohd A. , Khattak Saadullah , Shah Junaid Ali , Al-Keridis Lamya Ahmed , Alshammari Nawaf , Saeed Mohd , Igorevich Sadykov Aslan
Published: November 2023

Introduction

Zinc oxide nanoparticles (ZnO-NPs) have garnered considerable interest in biomedical research primarily owing to their prospective therapeutic implications in combatting pathogenic diseases and microbial infections. The primary objective of this study was to examine the biosynthesis of zinc oxide nanowhiskers (ZnO-NWs) using chicken egg white (albumin) as a bio-template. Furthermore, this study aimed to explore the potential biomedical applications of ZnO NWs in the context of infectious diseases.

Methods

The NWs synthesized through biological processes were observed using electron microscopy, which allowed for detailed examination of their characteristics. The results of these investigations indicated that the NWs exhibited a size distribution ranging from approximately 10 to 100 nm. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) mapping analyses successfully corroborated the size, dimensions, and presence of biological constituents during their formation. In this study, XTT assay and confocal imaging were employed to provide evidence of the efficacy of ZnO-NWs in the eradication of bacterial biofilms. The target bacterial strains were Staphylococcus aureus and Escherichia coli. Furthermore, we sought to address pertinent concerns regarding the biocompatibility of the ZnO-NWs. This was achieved through comprehensive evaluation of the absence of cytotoxicity in normal HEK-293T and erythrocytes.

Results

The findings of this investigation unequivocally confirmed the biocompatibility of the ZnO-NWs. The biosynthesized ZnO-NWs demonstrated a noteworthy capacity to mitigate the dermatitis-induced consequences induced by Staphylococcus aureus in murine models after a therapeutic intervention lasting for one week.

Discussion

This study presents a comprehensive examination of the biosynthesis of zinc oxide nanowhiskers (ZnO-NWs) derived from chicken egg whites. These findings highlight the considerable potential of biosynthesized ZnO-NWs as a viable option for the development of therapeutic agents targeting infectious diseases. The antibacterial efficacy of ZnO-NWs against both susceptible and antibiotic-resistant bacterial strains, as well as their ability to eradicate biofilms, suggests their promising role in combating infectious diseases. Furthermore, the confirmed biocompatibility of ZnO-NWs opens avenues for their safe use in biomedical applications. Overall, this research underscores the therapeutic promise of ZnO-NWs and their potential significance in future biomedical advancements.
A retrospective analysis investigating the effects of Telazol® and medetomidine on ejaculate characteristics in cheetahs (Acinonyx jubatus)
Authors: Baquerizo Carolina I. , Penfold Linda M. , Gillis James D. , Citino Scott , Marker Laurie , Crosier Adrienne E.
Published: November 2023

Zoo managed cheetahs provide an insurance population for wild cheetahs that are under threat of extinction from habitat loss, lack of prey, competition, pet trade and poaching for skin and bones. Assisted reproductive techniques including artificial insemination, in vitro fertilization, and embryo transfer augment natural breeding programs but rely on good quality semen for best results. It is understood that anesthesia can affect semen characteristics such as ejaculate volume, total sperm count, sperm motility, and incidence of urine contamination. Thus, the aim of this study was to conduct a retrospective analysis of 23 years of data to investigate sperm parameters of semen collected under anesthesia using medetomidine in combination with butorphanol and midazolam or Telazol® alone. Electroejaculation records (Medetomidine, Butorphanol, and Midazolam anesthetized n = 59 ejaculates, from 30 cheetahs, Telazol® anesthetized, n= 169 ejaculates, from 72 cheetahs) were evaluated for incidence of urine contamination. Electroejaculation records (Medetomidine, Butorphanol, and Midazolam anesthetized n = 21 ejaculates, from 17 cheetahs, Telazol® anesthetized, n = 143 ejaculates, from 63 cheetahs) were evaluated for total sperm count, total motility, ejaculate volume, and testicle size. Telazol® treated cheetahs had a numerically higher total sperm count (Median ± SD: 42.58 ± 77.8 × 106 spermatozoa) compared to those treated with medetomidine (Median ± SD: 31.2 ±44.58 × 106 spermatozoa), and a significantly (p < 0.05) higher sperm motility (Median ± SD: 70.0 ± 9.71%) compared to medetomidine (Median ± SD: 53.0 ± 16.41%) treated cheetahs. The findings of this study indicate that medetomidine anesthesia results in significantly lower sperm motility and Telazol® anesthesia results in a higher total sperm count and motility, thus resulting in higher quality ejaculate. This information can aid in the veterinary management of the species when involved in genome resource banking and assisted reproductive technologies.
Rwandan teacher training college’s mathematics teachers’ pedagogical content knowledge for teaching: assessment toward competency-based curriculum
Authors: Habiyaremye Hashituky Telesphore , Ntivuguruzwa Celestin , Ntawiha Philothere
Published: November 2023

Introduction

Teachers’ knowledge and positive attitudes significantly impact educational settings. This study aimed to assess teachers’ mathematical knowledge for teaching (MKT) and their pedagogical content knowledge (PCK) attitudes.

Methodology

It was conducted on all 23 mathematics teachers from Rwandan teacher training colleges (TTCs). Employing a survey design, standardized performance and attitude tests were utilized, employing instruments known for their standardization, validity, and reliability.

Results

The findings informed that teachers possess a low MKT but a high PCK attitude. Thus, they performed the MKT test poorly as the average score was below 50%. However, teachers were found to have better content knowledge than other MKT constructs and performed better in pattern function and algebra than in other areas of mathematics. Teachers demonstrated a highly positive attitude toward integrating PCK in teaching mathematics.

Discussion

Such a positive attitude was inferred from the pedagogical training offered by implementing a competency-based curriculum. Therefore, teachers need to improve their MKT performance, and more research is needed on other teachers apart from those in TTCs.
Suitability assessments in teacher education during and post the COVID-19 pandemic – an impact on professional development
Authors: Hvalby Mette
Published: November 2023

Introduction

Suitability assessment of Norwegian pre-service teachers is carried out by teacher educators, and the mentors’ professional practices play a key role in these processes. This study aims to explore how the Covid-19 pandemic has influenced suitability assessment practices of pre-service teachers. The purpose of the suitability assessment is to ensure that vulnerable groups, such as students in schools, encounter teachers who are suitable for a professional practice. The process of growing suitability includes professional development, while suitability assessment of pre-service teachers intends to strengthen the quality of teacher education programs.

Methods

Two sets of data collection methods are underpinning the arguments in this qualitative study. Data were collected in 2022 from a survey with a sample of 162 mentors in teacher education attending three universities. Thereafter, in-depth interviews were conducted with three Heads of suitability assessment from the same universities as the mentors. The triangulation aimed to reduce bias and increase the validity.

Results

The impact the pandemic has had on the suitability assessment practices is expressed in four findings: (1) a weaker basis for the assessment, (2) continuing health issues, (3) delayed professional development among pre-service teachers, and (4) differences among the universities.

Recommendations

Implications for education programs for mentoring including knowledge of the procedures of suitability assessments, and a closer collaboration between all teacher educators involving systematically evaluation of pre-service teachers are indicated for future teacher education.
Renewing an undergraduate science curriculum for the 21st century
Authors: McAteer Louise , Roche Joseph , Kelly Áine M.
Published: November 2023

Introduction

The rapid pace of technological advancement, globalisation, and complex socio-economic challenges facing 21st-century society necessitates a rethinking of undergraduate science education. Undergraduate science curriculum reform is essential to prepare students for the demands of the modern workforce in an ever-changing world. Accordingly, in Trinity College Dublin (the University of Dublin), the oldest science degree course in Ireland was intensively reviewed and redeveloped between 2014 and 2021. This study aims to collate and disseminate the knowledge acquired by university staff through the experience of undertaking a major science curriculum redevelopment.

Methods

Nine senior staff members closely involved with the redevelopment were interviewed about why the curriculum reform was necessary, what it had achieved, and how the process could have been improved.

Results

The reasons behind the curriculum changes are described and placed in the context of contemporary pedagogical research. Reflections from the academic and administrative staff involved in the redevelopment process are presented, emphasising the challenges and opportunities that emerged from that process.

Discussion

Recommendations for other universities undertaking similar reforms are included. Aligning undergraduate science curriculum reform with the needs of 21st-century society is vital for ensuring that science graduates are well-prepared to make positive contributions to a sustainable future.
Constructing a nomogram based on the distribution of thyroid nodules and suspicious lateral cervical lymph nodes in fine-needle aspiration biopsies to predict metastasis in papillary thyroid carcinoma
Authors: Liu Shui-Qing , Feng Jia-Wei , Yan Zhan-Tao , Xing Xiao-Xiao , Jiang Wen-Yin , Jiang Yong , Qian Feng , Xing Wei
Published: November 2023

Purpose

Elevated concentrations of thyroglobulin eluent is a risk factor for lateral cervical lymph node metastasis (LLNM) in patients with papillary thyroid cancer (PTC). We aimed to develop a practical nomogram based on the distribution of thyroid nodules and the presence of suspicious lateral cervical lymph nodes in fine-needle aspiration biopsies (LN-FNABs), including the cytopathology and the suspicious lateral cervical lymph node (LLN) thyroglobulin eluent (Tg), to predict the possibility of LLNM preoperatively in patients with PTC.

Methods

The clinical data of PTC patients who were admitted to the Third Affiliated Hospital of Soochow University from January 2022 to May 2023 to undergo fine-needle aspiration biopsy (FNAB) were included in this study. A total of 208 patients in 2022 served as the training set (70%), and 89 patients in 2023 served as the validation set (30%). The clinical characteristics and LN-FNAB results were collected to determine the risk factors of LLNM. A preoperative nomogram was developed for predicting LLNM based on the results of the univariate and multivariate analyses. Internal calibration, external calibration, and decision curve analysis (DCA) were performed for these models.

Results

The multivariate logistic regression analysis showed that the maximum thyroid nodule diameter (Odds Ratio (OR) 2.323, 95% CI 1.383 to 3.904; p = 0.001), Tg level (OR 1.007, 95% CI 1.005 to 1.009; p = 0.000), Tg divided by serum thyroglobulin, (Tg/sTg) [odds ratio (OR) 1.005, 95% CI 1.001 to 1.008; p = 0.009], and cytopathology (OR 9.738, 95% CI 3.678 to 25.783; p = 0.000) (all p <  0.05) had a significant impact on the LLNM of patients with suspicious LLNs. The nomogram showed a better predictive value in both the training cohort [area under the curve, (AUC) 0.937, 95% CI 0.895 to 0.966] and the validation cohort (AUC 0.957, 95% CI 0.892 to 0.989). The nomogram also showed excellent internal and external calibration in predicting LLNM. According to the DCA, the diagnostic performance of this model was dependent on the following variables: maximum thyroid nodule diameter, Tg level, Tg/sTg, and cytopathology.

Conclusion

Based on the aforementioned risk factors, we believe that it is necessary to establish a personalized LLNM model for patients with PTC. Using this practical nomogram, which combines clinical and Tg risk factors, surgeons could accurately predict the possibility of LLNM preoperatively. The nomogram will also help surgeons to establish personalized treatment plans before surgery.
Robotic posterior retroperitoneal adrenalectomy versus laparoscopic posterior retroperitoneal adrenalectomy: outcomes from a pooled analysis
Authors: Li Yu-gen , Chen Xiao-bin , Wang Chun-mei , Yu Xiao-dong , Deng Xian-zhong , Liao Bo
Published: November 2023

Background

The comparative advantages of robotic posterior retroperitoneal adrenalectomy (RPRA) over laparoscopic posterior retroperitoneal adrenalectomy (LPRA) remain a topic of ongoing debate within the medical community. This systematic literature review and meta-analysis aim to assess the safety and efficacy of RPRA compared to LPRA, with the ultimate goal of determining which procedure yields superior clinical outcomes.

Methods

A systematic search was conducted on databases including PubMed, Embase, Web of Science, and the Cochrane Library database to identify relevant studies, encompassing both randomized controlled trials (RCTs) and non-RCTs, that compare the outcomes of RPRA and LPRA. The primary focus of this study was to evaluate perioperative surgical outcomes and complications. Review Manager 5.4 was used for this analysis. The study was registered with PROSPERO (ID: CRD42023453816).

Results

A total of seven non-RCTs were identified and included in this study, encompassing a cohort of 675 patients. The findings indicate that RPRA exhibited superior performance compared to LPRA in terms of hospital stay (weighted mean difference [WMD] -0.78 days, 95% confidence interval [CI] -1.46 to -0.10; p = 0.02). However, there were no statistically significant differences observed between the two techniques in terms of operative time, blood loss, transfusion rates, conversion rates, major complications, and overall complications.

Conclusion

RPRA is associated with a significantly shorter hospital stay compared to LPRA, while demonstrating comparable operative time, blood loss, conversion rate, and complication rate. However, it is important to note that further research of a more comprehensive and rigorous nature is necessary to validate these findings.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=453816, identifier CRD42023453816.
A statistical examination of the link between environmental performance and legal practices: an evaluation of China’s strategies for residual legislative power allocation
Authors: Tian Ze-Hua , Yang Wu-Song , Tan Cheng-Xue
Published: November 2023

This study investigates the correlation between the environmental legal practices of different countries and their environmental performance. It entails an empirical analysis of cross-sectional environmental data collected from 34 countries, including members of the Organization for Economic Cooperation and Development (OECD) and the BRICs nations (Brazil, Russia, India, China, and South Africa). Then the study explores the correlation between a country’s environmental performance and both the environmental policy stringency and regulatory enforcement. The findings from this global assessment are subsequently corroborated through an examination of China’s environmental time series data spanning a decade, revealing a significant relationship between a country’s environmental performance and regulatory enforcement. These results validate the Incomplete Law Theory within the field of environmental law. Moreover, as the second most populous and the third-largest country in terms of land area globally, China’s environmental protection strategies and performance play a pivotal role in influencing international environmental outcomes. Consequently, the study conducts a case study on China’s environmental legal practices and provides suggestions for enhancing China’s allocation strategies of residual legislative power. The study advocates for the optimization of residual legislative power allocation within local environmental law enforcement agencies and a balanced distribution of public and private residual legislative power. This approach reinforces the government’s role in strategic formulation.
Assessing the invasion risk of Chelydra serpentina in China under current and future climate change scenarios
Authors: Mu Chaosheng , Li Pipeng
Published: November 2023

Chelydra serpentina, a species introduced to China for aquaculture purposes, is commonly found in its natural habitats within the country. The invasion of C. serpentina poses potential threats to both the biodiversity of China and human health. The potential distribution of C. serpentina has been simulated using the species distribution model – MaxEnt, incorporating global distribution data, climate, and land cover variables. Our simulations encompasses both current conditions and four future climate change scenarios. Currently, the potential distribution is concentrated in central, eastern, and southeastern regions of China, with the central and eastern regions facing the highest risk of invasion. Under future climate change scenarios, the distribution area may expand by 30–90%, and multiple provinces will face a more severe threat of invasion. This study presents the inaugural simulation of the potential invasion range of C. serpentina under current climatic conditions. Moreover, it reveals that climate change is likely to contribute to the expansion of its invasive range, thus furnishing a reference foundation for scientific prevention and control measures. We propose integrating citizen science and eDNA technologies into species monitoring to enhance the efficiency of detecting invasive species. This research has filled the gap in the research on the invasive distribution range of C. serpentina in China and globally, while also providing novel perspectives on the invasion control of this species.
A conceptual analysis of public opinion regarding genome research in Japan
Authors: Shahrier Shibly , Gaydarska Hristina , Takashima Kayo , Yoshizawa Go , Minari Jusaku
Published: November 2023

In the 20 years since the completion of the Human Genome Project, the gap between scientific development and public understanding of genome research has been widening. While genome research has been increasingly utilized for social and clinical purposes in a multifaceted manner, this has resulted in an increase in the potential risks associated with genomic data. In this context, our study aims to consider the nature of public perceptions of genome research, primarily by using as a case study the results of previous public surveys relevant to donations for social benefits in Japan. We explored certain types of awareness, attitude, and intention (A-A-I) in such surveys and discussed the resultant key findings through the cultural transmission framework. Reframing the public’s response toward genome research based on A-A-I analysis and behavioral science may contribute to developing more systematic communication approaches with the public. With a view to establishing such approaches, our perspective suggests some new insights to discuss the science–society gap in genome research internationally.
B cell MHC haplotype affects follicular inclusion, germinal center participation and plasma cell differentiation in a mouse model of lupus
Authors: Wibrand Camilla , Wittenborn Thomas R. , Voss Lasse Frank , Winther Gudrun , Jensen Lisbeth , Ferapontov Alexey , Fonager Sofie V. , Fahlquist-Hagert Cecilia , Degn Søren E.
Published: November 2023

Introduction

MHC class II molecules are essential for appropriate immune responses against pathogens but are also implicated in pathological responses in autoimmune diseases and transplant rejection. Previous studies have shed light on the systemic contributions of MHC haplotypes to the development and severity of autoimmune diseases. In this study, we addressed the B cell intrinsic MHC haplotype impact on follicular inclusion, germinal center (GC) participation and plasma cell (PC) differentiation in the context of systemic lupus erythematosus (SLE).

Methods

We leveraged the 564Igi mouse model which harbors a B cell receptor knock-in from an autoreactive B cell clone recognizing ribonuclear components, including double-stranded DNA (dsDNA). This model recapitulates the central hallmarks of the early stages of SLE. We compared 564Igi heterozygous offspring on either H2b/b, H2b/d, or H2d/d background.

Results

This revealed significantly higher germinal center (GC) B cell levels in the spleens of H2b/b and H2b/d as compared to H2d/d (p<0.0001) mice. In agreement with this, anti-dsDNA-antibody levels were higher in H2b/b and H2b/d than in H2d/d (p<0.0001), with H2b/b also being higher compared to H2b/d (p<0.01). Specifically, these differences held true both for autoantibodies derived from the knock-in clone and from wild-type (WT) derived clones. In mixed chimeras where 564Igi H2b/b, H2b/d and H2d/d cells competed head-to-head in the same environment, we observed a significantly higher inclusion of H2b/b cells in GC and PC compartments relative to their representation in the B cell repertoire, compared to H2b/d and H2d/d cells. Furthermore, in mixed chimeras in which WT H2b/b and WT H2d/d cells competed for inclusion in GCs associated with an epitope spreading process, H2b/b cells participated to a greater extent and contributed more robustly to the PC compartment. Finally, immature WT H2b/b cells had a higher baseline of BCRs with an autoreactive idiotype and were subject to more stringent negative selection at the transitional stage.

Discussion

Taken together, our findings demonstrate that B cell intrinsic MHC haplotype governs their capacity for participation in the autoreactive response at multiple levels: follicular inclusion, GC participation, and PC output. These findings pinpoint B cells as central contributors to precipitation of autoimmunity.
Risk factors and outcomes of IgA nephropathy recurrence after kidney transplantation: a systematic review and meta-analysis
Authors: Li Yue , Tang Yangming , Lin Tao , Song Turun
Published: November 2023

Background

IgA nephropathy may recur in patients receiving kidney transplantation due to IgA nephropathy induced renal failure. The risk factors for recurrence are still at issue. The aim of this study was to conduct a systematic review and meta-analysis to assess risk factors and outcomes for IgA nephropathy recurrence.

Methods

We used PubMed, EMBASE, Cochrane Library, Web of Science, Scopus, CNKI, WanFang, VIP and CBM to search for relevant studies published in English and Chinese. Cohort or case-control studies reporting risk factors or outcomes for IgA nephropathy recurrence were included.

Results

Fifty-eight studies were included. Compare to no recurrence group, those with IgAN recurrence had younger age (mean difference [MD]=-4.27 years; risk ratio [RR]=0.96), younger donor age (MD=-2.19 years), shorter time from IgA nephropathy diagnosis to end stage renal disease (MD=-1.84 years; RR=0.94), shorter time on dialysis (MD=-3.14 months), lower human leukocyte-antigen (HLA) mismatches (MD=-0.11) and HLA-DR mismatches (MD=-0.13). HLA-B46 antigen (RR=0.39), anti-IL-2-R antibodies induction (RR=0.68), mycophenolate mofetil (RR=0.69), and pretransplant tonsillectomy (RR=0.43) were associated with less IgAN recurrence. Of note, male recipient gender (RR=1.17), related donor (RR=1.53), retransplantation (RR=1.43), hemodialysis (RR=1.68), no induction therapy (RR=1.73), mTOR inhibitor (RR=1.51), angiotensin-converting enzyme inhibitors or angiotensin-receptor blockers (RR=1.63) were risk factors for IgAN recurrence. Recurrence increased the risk of graft loss (RR=2.19).

Conclusions

This study summarized the risk factors for recurrence of IgA nephropathy after kidney transplantation. Well-designed prospective studies are warranted for validation.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=377480, identifier CRD42022377480.
Regulatory mechanism of macrophage polarization based on Hippo pathway
Authors: Liu Yuanqing , An Yina , Li Gebin , Wang Shuaiyu
Published: November 2023

Macrophages are found to infiltrate and migrate in a large number of Tumor-associated macrophages (TMEs) and other macrophages in the microenvironment of tumors and related diseases, and undergo phenotypic changes in response to a variety of cytokines, mainly including the primary phenotype M2 and the anti-tumor phenotype M1. The Hippo signaling pathway affects the development of cancer and other diseases through various biological processes, such as inhibition of cell growth. In this review, we focus on immune cells within the microenvironment of tumors and other diseases, and the role of the Hippo pathway in tumors on macrophage polarization in the tumor microenvironment (TME) and other diseases.
Mapping the citation network on vitamin D research in Australia: a data-driven approach
Authors: Neo Belinda , Qu Xiaochen , Dunlop Eleanor , Shepherd Carrington , Walsh Erin I. , Cherbuin Nicolas , Black Lucinda J.
Published: November 2023

Vitamin D research can vary geographically, as vitamin D status is influenced by latitude, season, dietary intake, body mass index, ethnicity, and public health initiatives. Over the last two decades, research on vitamin D has increased in Australia, where the potential for sun exposure (a major source of vitamin D) is high. We aimed to identify key topics and gaps in vitamin D research in Australia using a data-driven approach. A literature search limited to Australian studies was conducted in the Web of Science Core Collection database. Citation network analysis was conducted to identify clusters and sub-clusters, depicted using word clouds. Topic analysis of each cluster and sub-cluster was conducted to identify topics and sub-topics, respectively. From 934 publications (over the period 1984–2022), nine topics and 60 sub-topics were identified. The nine topics were: vitamin D in vulnerable populations and its impact on child development; impact of sun exposure and ultraviolet-B radiation on various health conditions; vitamin D and falls and fractures in older adults; vitamin D and its association with health outcomes; vitamin D from sun exposure; testing of vitamin D status in Australia; vitamin D, calcium, and musculoskeletal health; vitamin D status and knee osteoarthritis; and vitamin D status and exercise performance in athletes. There were limited publications on vitamin D in Aboriginal and Torres Strait Islander peoples and dietary vitamin D. We have provided an overview of vitamin D research in Australia. The research trends and knowledge gaps identified can guide future research to better inform public health initiatives in Australia.
Understanding voluntary human movement variability through data-driven segmentation and clustering
Authors: Daneault Jean-Francois , Oubre Brandon , Miranda Jose Garcia Vivas , Lee Sunghoon Ivan
Published: November 2023

Recently, we proposed a novel approach where movements are decomposed into sub-segments, termed movement elements. This approach, to date, provides a robust construct of how the brain may generate simple as well as complex movements. Here, we address the issue of motor variability during voluntary movements by applying an unsupervised clustering algorithm to group movement elements according to their morphological characteristics. We observed that most movement elements closely match the theoretical bell-shaped velocity profile expected from goal-directed movements. However, for those movement elements that deviate from this theoretical shape, a small number of defined patterns in their shape can be identified. Furthermore, we observed that the axis of the body from which the movement elements are extracted (i.e., medio-lateral, antero-posterior, and vertical) affect the proportion of the movement elements matching the theoretical model. These results provide novel insight into how the nervous system controls voluntary movements and may use variability in movement element properties to explore the environment.
Seasonal and nutritional changes in the short form of the leptin receptor expression and VEGF system in the choroid plexus, arcuate nucleus, and anterior pituitary in MTS-leptin and resistin-treated sheep
Authors: Zieba Dorota Anna , Biernat Weronika , Szczesna Malgorzata , Kirsz Katarzyna , Misztal Tomasz
Published: November 2023

The short form of the leptin receptor (LeptRa) plays a key role in the transport of leptin to the central nervous system (CNS). Here, MTS-leptin and recombinant ovine (ro) leptin-mediated expression of LeptRa and VEGFA and VEGFR2 concentration in selected hypothalamic nuclei, choroid plexus (ChP), and anterior pituitary (AP) were analyzed considering the photoperiod and acute-fasting (experiment 1), and nutritional status (experiment 2) of ewes. In experiment 1, 60 sheep were fed normally or fasted for 72 h and received one injection of saline, MTS-leptin, or roleptin 1 h prior to euthanasia. LeptRa mRNA transcript levels and VEGF system protein concentrations were detected in the ARC, ChP predominantly in the SD, and AP for the LD without detection of LeptRa in the POA and VMH/DMH. In experiment 2, an altered diet for 5 months created lean or fat sheep. Twenty sheep were divided into four groups: the lean and fat groups were given saline, while the lean-R and fat-R groups received resistin 1 h prior to euthanasia. Changes in adiposity influenced the lowering effect of resistin on the expression of LeptRa and VEGF system protein concentrations. Overall, both photoperiodic and nutritional signals influence the effects of MTS-leptin/roleptin and resistin-mediated leptin transport to the CNS via LeptRa. Resistin seems to be another adipokine involved in the adaptive/pathological phenomenon of leptin resistance in sheep.
Sulforaphane as a potential modifier of calorie-induced inflammation: a double-blind, placebo-controlled, crossover trial
Authors: van Steenwijk Hidde P. , Vinken Anna , van Osch Frits H. M. , Peppelenbos Herman , Troost Freddy J. , Bast Aalt , Semen Khrystyna O. , de Boer Alie
Published: November 2023

Background and aims

Observational data indicate that diets rich in fruits and vegetables have a positive effect on inflammatory status, improve metabolic resilience and may protect against the development of non-communicable diseases. Nevertheless, experimental evidence demonstrating a causal relationship between nutrient intake (especially whole foods) and changes in metabolic health is scarce. This study investigated the pleiotropic effects of sulforaphane from broccoli sprouts, compared to pea sprouts, on biomarkers of endothelial function, inflammation and metabolic stress in healthy participants subjected to a standardized caloric challenge.

Methods

In this double-blind, crossover, randomized, placebo-controlled trial 12 healthy participants were administered 16 g broccoli sprouts, or pea sprouts (placebo) followed by the standardized high-caloric drink PhenFlex given to disturb healthy homeostasis. Levels of inflammatory biomarkers and metabolic parameters were measured in plasma before and 2 h after the caloric overload.

Results

Administration of broccoli sprouts promoted an increase in levels of CCL-2 induced by caloric load (p = 0.017). Other biomarkers (sICAM-1, sVCAM-1, hs-CRP, and IL-10) individually showed insignificant tendencies toward increase with administration of sulforaphane. Combining all studied biomarkers into the systemic low-grade inflammation score further confirmed upregulation of the inflammatory activity (p = 0.087) after sulforaphane. No significant effects on biomarkers of metabolic stress were detected.

Conclusion

This study has demonstrated that sulforaphane facilitated development of a mild pro-inflammatory state during the caloric challenge, which could be suggestive of the onset of the hormetic response induced by this phytonutrient. The use of integrative outcomes measures such as the systemic low-grade inflammation score can be viewed as a more robust approach to study the subtle and pleiotropic effects of phytonutrients.

Clinical trial registration:www.clinicaltrials.gov, identifier NCT05146804.
Current tests for diagnosis of hepatitis B virus infection and immune responses of HBV-related HCC
Authors: Shi Wanting , Li Kang , Zhang Yonghong
Published: November 2023

Chronic hepatitis B virus (HBV) infection is a worldwide public health threat that results in huge morbidity and mortality. Late diagnosis and delayed treatment of HBV infections can cause irreversible liver damages and occurrence of cirrhosis and hepatocellular carcinoma (HCC). Detection of the presence and activity of HBV are the cornerstones of diagnosis and management in HBV related disease. Moreover, comprehensive knowledge of the mechanisms regulating HBV immunobiology is pivotal for managing diseases related with HBV. Here we tried to categorize and illustrate the classical and novel approaches used for diagnosis of HBV. Also, we reviewed our current knowledge on the immunobiology of HBV related HCC.