bluuebunny/crossref_metadata_2025 huggingface dataset:
Acute dairy milk ingestion does not improve nitric oxide-dependent vasodilation in the cutaneous microcirculation
Authors: Alba Billie K. , Stanhewicz Anna E. , Kenney W. Larry , Alexander Lacy M.
Published: May 2016

In epidemiological studies, chronic dairy milk consumption is associated with improved vascular health and reduced age-related increases in blood pressure. Although milk protein supplementation augments conduit artery flow-mediated dilation, whether or not acute dairy milk intake may improve microvascular function remains unclear. We hypothesised that dairy milk would increase direct measurement of endothelial nitric oxide (NO)-dependent cutaneous vasodilation in response to local skin heating. Eleven men and women (61 (sem2) years) ingested two or four servings (473 and 946 ml) of 1 % dairy milk or a rice beverage on each of 4 separate study days. In a subset of five subjects, an additional protocol was completed after 473 ml of water ingestion. Once a stable blood flow occurred, 15 mm-NG-nitro-l-arginine methyl ester was perfused (intradermal microdialysis) to quantify NO-dependent vasodilation. Red-blood-cell flux (RBF) was measured by laser-Doppler flowmetry, and cutaneous vascular conductance (CVC=RBF/mean arterial pressure) was calculated and normalised to maximum (%CVCmax; 28 mm-sodium nitroprusside). Full expression of cutaneous vasodilation was not different among dairy milk, rice beverage and water, and there was no effect of serving size on the total vasodilatory response. Contrary to our hypothesis, NO-dependent vasodilation was lower for dairy milk than rice beverage (D: 49 (sem5), R: 55 (sem5) %CVCmax;P<0·01). Acute dairy milk ingestion does not augment NO-dependent vasodilation in the cutaneous microcirculation compared with a rice beverage control.
Modelling harvest of Asian elephantsElephas maximuson the basis of faulty assumptions promotes inappropriate management solutions
Authors: Puyravaud Jean Philippe , Davidar Priya , Srivastava Rajeev K. , Wright Belinda
Published: May 2016

A ratio-based logistic model developed to assess elephant harvest rates, based on a study at Nagarhole Tiger Reserve in India, was recommended as a management tool to control human–elephant conflict through culling. Considering this reserve among others violates an assumption of the logistic model: isolation. Nevertheless, assuming this violation was irrelevant, we re-evaluated the model, with minor modifications, for the neighbouring Mudumalai Tiger Reserve, where we used data from 13 elephantElephas maximuspopulation surveys to derive bootstrapped sets of population ratios, and mortality records. We generated arrays of harvest regimes and examined which ratio outputs were closest to the bootstrapped ratios. Our results indicated that (1) model outputs corresponded best with the Mudumalai population structure when harvest regimes were extreme and unlikely, (2) there were significant differences in population structure and harvest regimes between Nagarhole and Mudumalai, and (3) only 49% of adult male deaths predicted by model outputs were recorded in official governmental records. The model provides significantly different results among reserves, which invalidates it as a tool to predict change across the entire elephant population. Variability in survey data and inaccuracies in transition probabilities are sufficiently large to warrant caution when using them as a basis for deterministic modelling. Official mortality databases provide a weak means of validation because poaching incidents are poorly recorded. We conclude that the model should be based on validated transition probabilities and encompass the entire regional population.
Detection of the European epidemic strain of Trichomonas gallinae in finches, but not other non-columbiformes, in the absence of macroscopic disease
Authors: ZU ERMGASSEN ERASMUS K.H.J. , DURRANT CHRIS , JOHN SHINTO , GARDINER ROXANNE , ALREFAEI ABDULWAHED F. , CUNNINGHAM ANDREW A. , LAWSON BECKI
Published: May 2016

Finch trichomonosis is an emerging infectious disease affecting European passerines caused by a clonal strain of Trichomonas gallinae. Migrating chaffinches (Fringilla coelebs) were proposed as the likely vector of parasite spread from Great Britain to Fennoscandia. To test for such parasite carriage, we screened samples of oesophagus/crop from 275 Apodiform, Passeriform and Piciform birds (40 species) which had no macroscopic evidence of trichomonosis (i.e. necrotic ingluvitis). These birds were found dead following the emergence of trichomonosis in Great Britain, 2009–2012, and were examined post-mortem. Polymerase chain reactions were used to detect (ITS1/5·8S rRNA/ITS2 region and single subunit rRNA gene) and to subtype (Fe-hydrogenase gene) T. gallinae. Trichomonas gallinae was detected in six finches [three chaffinches, two greenfinches (Chloris chloris) and a bullfinch (Pyrrhula pyrrhula)]. Sequence data had 100% identity to the European finch epidemic A1 strain for each species. While these results are consistent with finches being vectors of T. gallinae, alternative explanations include the presence of incubating or resolved T. gallinae infections. The inclusion of histopathological examination would help elucidate the significance of T. gallinae infection in the absence of macroscopic lesions.
Heart rate variability and depressive symptoms: a cross-lagged analysis over a 10-year period in the Whitehall II study
Authors: Jandackova V. K. , Britton A. , Malik M. , Steptoe A.
Published: May 2016

Background

People with depression tend to have lower heart rate variability (HRV), but the temporal sequence is poorly understood. In a sample of the general population, we prospectively examined whether HRV measures predict subsequent depressive symptoms or whether depressive symptoms predict subsequent levels of HRV.

Method

Data from the fifth (1997–1999) and ninth (2007–2009) phases of the UK Whitehall II longitudinal population-based cohort study were analysed with an average follow-up of 10.5 years. The sample size for the prospective analysis depended on the analysis and ranged from 2334 (644 women) to 2276 (602 women). HRV measures during 5 min of supine rest were obtained. Depressive symptoms were evaluated by four cognitive symptoms of depression from the General Health Questionnaire.

Results

At follow-up assessment, depressive symptoms were inversely associated with HRV measures independently of antidepressant medication use in men but not in women. Prospectively, lower baseline heart rate and higher HRV measures were associated with a lower likelihood of incident depressive symptoms at follow-up in men without depressive symptoms at baseline. Similar but statistically insignificant associations were found in women. Adjustments for known confounders including sociodemographic and lifestyle factors, cardiometabolic conditions or medication did not change the predictive effect of HRV on incident depressive symptoms at follow-up. Depressive symptoms at baseline were not associated with heart rate or HRV at follow-up in either sex.

Conclusions

These findings are consistent with an aetiological role of the autonomic nervous system in depression onset.
An object-oriented study on Yongwu shi: poetry on eyeglasses in the Qing dynasty
Authors: Yan Zinan
Published: May 2016

This paper suggests a different approach in the study of Qing dynasty yongwu poetry, which is to analyse the cultural significance of the object in this literary subgenre instead of its lyrical essence. Taking eyeglasses as an example, this paper surveys the general development of imported and domestic eyeglasses in China from the fifteenth to the nineteenth centuries, and presents various literary interpretations assigned to this object through time. It further discusses Western and Chinese materiality, the economy and scholars' lives, political and social justification, as well as literary complexity and object identity, which are vital to the development of poetry on eyeglasses in the late imperial period.
We Need to Talk about Impact: Why Social Policy Academics need to Engage with the UK's Research Impact Agenda
Authors: SMITH KATHERINE E. , STEWART ELLEN
Published: May 2016

Of all the social sciences, social policy is one of the most obviously policy-orientated. One might, therefore, expect a research and funding agenda which prioritises and rewards policy relevance to garner an enthusiastic response among social policy scholars. Yet, the social policy response to the way in which major funders and the Research Excellence Framework (REF) are now prioritising ‘impact’ has been remarkably muted. Elsewhere in the social sciences, ‘research impact’ is being widely debated and a wealth of concerns about the way in which this agenda is being pursued are being articulated. Here, we argue there is an urgent need for social policy academics to join this debate. First, we employ interviews with academics involved in health inequalities research, undertaken between 2004 and 2015, to explore perceptions, and experiences, of the ‘impact agenda’ (an analysis which is informed by a review of guidelines for assessing ‘impact’ and relevant academic literature). Next, we analyse high- and low-scoring REF2014 impact case studies to assess whether these concerns appear justified. We conclude by outlining how social policy expertise might usefully contribute to efforts to encourage, measure and reward research ‘impact’.
Existence of positive solutions for a semipositone p-Laplacian problem
Authors: Castro Alfonso , de Figueredo Djairo G. , Lopera Emer
Published: May 2016

We prove the existence of positive solutions to a semipositone p-Laplacian problem combining mountain pass arguments, comparison principles, regularity principles and a priori estimates.
The winter distribution of the Spoon-billed Sandpiper Calidris pygmaeus
Authors: ZÖCKLER CHRISTOPH , BERESFORD ALISON E. , BUNTING GILLIAN , CHOWDHURY SAYAM U. , CLARK NIGEL A. , FU VIVIAN WING KAN , HTIN HLA TONY , MOROZOV VLADIMIR V. , SYROECHKOVSKIY EVGENY E. , KASHIWAGI MINORU , LAPPO ELENA G. , TONG MENXIU , LONG TRAI LE , YU YAT-TUNG , HUETTMANN FALK , AKASOFU HERRICK K. , TOMIDA HIROSHI , BUCHANAN GRAEME M.
Published: May 2016

Declines in populations of the Critically Endangered Spoon-billed Sandpiper Calidris pygmaeus have been rapid, with the breeding population now perhaps numbering fewer than 120 pairs. The reasons for this decline remain unresolved. Whilst there is evidence that hunting in wintering areas is an important factor, loss of suitable habitat on passage and wintering areas is also of concern. While some key sites for the species are already documented, many of their wintering locations are described here for the first time. Their wintering range primarily stretches from Bangladesh to China. Comprehensive surveys of potential Spoon-billed Sandpiper wintering sites from 2005 to 2013 showed a wide distribution with three key concentrations in Myanmar and Bangladesh, but also regular sites in China, Vietnam and Thailand. The identification of all important non-breeding sites remains of high priority for the conservation of the species. Here, we present the results of field surveys of wintering Spoon-billed Sandpipers that took place in six countries between 2005 and 2013 and present species distribution models which map the potential wintering areas. These include known and currently unrecognised wintering locations. Our maximum entropy model did not identify any new extensive candidate areas within the winter distribution, suggesting that most key sites are already known, but it did identify small sites on the coast of eastern Bangladesh, western Myanmar, and the Guangxi and Guangdong regions of China that may merit further investigation. As no extensive areas of new potential habitat were identified, we suggest that the priorities for the conservation of this species are habitat protection in important wintering and passage areas and reducing hunting pressure on birds at these sites.
Germination sensitivity to water stress of eight Cistaceae species from the Western Mediterranean
Authors: Luna Belén , Chamorro Daniel
Published: May 2016

Water availability regulates timing of germination, especially in those environments where it is a limiting factor. However, the water requirements for germination of most wild Mediterranean species are unknown. In this work we analysed the germination response to water stress of eight Cistaceae species with hard-coated seeds, which are typical for the Mediterranean shrublands. Seeds were exposed to a heat shock in order to overcome physical dormancy and then incubated under five water potentials (0, −0.2, −0.4, −0.6 and −0.8 MPa). Ungerminated seeds following these treatments were transferred to water in order to evaluate the recovery of germination. Additionally, at the end of the experiment, viability of still ungerminated seeds was examined. We analysed final germination percentage, time to reach 50% of the final germination (t50), recovery of germination and seed viability in relation to water stress treatments. Furthermore, hydrotime analysis was performed; sensitivity to water stress, as characterized by Ψb(50), was checked in relation to seed size of species. Overall, water stress delayed and decreased germination but species showed different germination sensitivities, which were not related to seed size. Recovery of germination was high after disappearance of water stress but seed viability decreased in some species. Consequently, post-fire germination of Cistaceae must be strongly regulated by water availability and their seeds can recover germination capacity when sufficient water is available after long periods of incomplete hydration. However, some seeds die during the wait.
Church asMbumbaand Bishop asNkhoswe: Anglican Ecclesiology and Missiological Imperatives in Central Africa
Authors: Mbaya Henry
Published: May 2016

This paper explores the use of the Chewa and Nyanja concepts ofmbumbaandnkhoswein Central Africa and Southern Africa as interpretive tools for an Anglican ecclesiology and theology of leadership. Following an exposition of these two concepts, it conceptualizesmbumbaas a diocese, and bishops asnkhoswederiving from Christ asNkhoswepar excellence. These two concepts entail critical values including responsibility, accountability and mutuality, which can be used as a model to enhance the relationship between a diocese and bishop. Conceptualizing a diocese asmbumbaand the role of a bishop as that ofnkhoswehas the potential to enhance missional practice in Central and Southern Africa.
The state of conflicts today: Can humanitarian action adapt?
Authors: McGoldrick Claudia
Published: May 2016

How do the dynamics of contemporary armed conflict shape, and constrain, humanitarian action? Is the international humanitarian “system”1 really at breaking point, as is often claimed? Or will it adapt to the changing realities not just of warfare but of global geopolitical shifts – as it has done repeatedly in the past – and evolve into something different? By way of response, the first part of this article offers a snapshot of today's armed conflicts and other situations of violence, focusing initially on the trends and features apparent in the Syrian conflict – which has in many ways come to define twenty-first-century warfare – and moving on to other countries and regions, many of which share at least some of these features, albeit in varying degrees. It considers the humanitarian consequences of today's armed conflicts and other situations of violence, and the implications for humanitarian response – which, at least on an international level, is indeed facing a watershed. The second part aims to show that even a glance back at key aspects of the evolution of humanitarian action over the past century – largely in response to the evolving nature of warfare and the developing international system – will remind us of quite radical changes in the face of major upheavals and challenges, not all of them dissimilar to those of today. The third part suggests that in today's global environment, international humanitarian response will continue to evolve and ultimately take on a different shape: one that reflects the changing nature of conflict and the geopolitical power shifts that go with it. With the rise of the global South, and the increasing recognition of the importance of local actors to humanitarian action, particular attention is given to the evolving relationship between local and international actors. In conclusion, the article reiterates some of the main reasons why humanitarian action – and international humanitarian actors in particular – will likely continue to adapt (albeit with varying degrees of success) to a changing world.
Xenon-mediated neuroprotection in response to sustained, low-level excitotoxic stress
Authors: Lavaur J , Lemaire M , Pype J , Nogue D Le , Hirsch E C , Michel P P
Published: May 2016

Noble gases such as xenon and argon have been reported to provide neuroprotection against acute brain ischemic/anoxic injuries. Herein, we wished to evaluate the protective potential of these two gases under conditions relevant to the pathogenesis of chronic neurodegenerative disorders. For that, we established cultures of neurons typically affected in Alzheimer's disease (AD) pathology, that is, cortical neurons and basal forebrain cholinergic neurons and exposed them to L-trans-pyrrolidine-2,4-dicarboxylic acid (PDC) to generate sustained, low-level excitotoxic stress. Over a period of 4 days, PDC caused a progressive loss of cortical neurons which was prevented substantially when xenon replaced nitrogen in the cell culture atmosphere. Unlike xenon, argon remained inactive. Xenon acted downstream of the inhibitory and stimulatory effects elicited by PDC on glutamate uptake and efflux, respectively. Neuroprotection by xenon was mimicked by two noncompetitive antagonists of NMDA glutamate receptors, memantine and ketamine. Each of them potentiated xenon-mediated neuroprotection when used at concentrations providing suboptimal rescue to cortical neurons but most surprisingly, no rescue at all. The survival-promoting effects of xenon persisted when NMDA was used instead of PDC to trigger neuronal death, indicating that NMDA receptor antagonism was probably accountable for xenon’s effects. An excess of glycine failed to reverse xenon neuroprotection, thus excluding a competitive interaction of xenon with the glycine-binding site of NMDA receptors. Noticeably, antioxidants such as Trolox and N-acetylcysteine reduced PDC-induced neuronal death but xenon itself lacked free radical-scavenging activity. Cholinergic neurons were also rescued efficaciously by xenon in basal forebrain cultures. Unexpectedly, however, xenon stimulated cholinergic traits and promoted the morphological differentiation of cholinergic neurons in these cultures. Memantine reproduced some of these neurotrophic effects, albeit with less efficacy than xenon. In conclusion, we demonstrate for the first time that xenon may have a therapeutic potential in AD.
Fast electronic resistance switching involving hidden charge density wave states
Authors: Vaskivskyi I. , Mihailovic I. A. , Brazovskii S. , Gospodaric J. , Mertelj T. , Svetin D. , Sutar P. , Mihailovic D.
Published: May 2016

The functionality of computer memory elements is currently based on multi-stability, driven either by locally manipulating the density of electrons in transistors or by switching magnetic or ferroelectric order. Another possibility is switching between metallic and insulating phases by the motion of ions, but their speed is limited by slow nucleation and inhomogeneous percolative growth. Here we demonstrate fast resistance switching in a charge density wave system caused by pulsed current injection. As a charge pulse travels through the material, it converts a commensurately ordered polaronic Mott insulating state in 1T–TaS2 to a metastable electronic state with textured domain walls, accompanied with a conversion of polarons to band states, and concurrent rapid switching from an insulator to a metal. The large resistance change, high switching speed (30 ps) and ultralow energy per bit opens the way to new concepts in non-volatile memory devices manipulating all-electronic states.
Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping
Authors: Augustsson Per , Karlsen Jonas T. , Su Hao-Wei , Bruus Henrik , Voldman Joel
Published: May 2016

Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells’ interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.
Pseudomonas aeruginosa elastase cleaves a C-terminal peptide from human thrombin that inhibits host inflammatory responses
Authors: van der Plas Mariena J. A. , Bhongir Ravi K. V. , Kjellström Sven , Siller Helena , Kasetty Gopinath , Mörgelin Matthias , Schmidtchen Artur
Published: May 2016

Pseudomonas aeruginosa is an opportunistic pathogen known for its immune evasive abilities amongst others by degradation of a large variety of host proteins. Here we show that digestion of thrombin by P. aeruginosa elastase leads to the release of the C-terminal thrombin-derived peptide FYT21, which inhibits pro-inflammatory responses to several pathogen-associated molecular patterns in vitro and in vivo by preventing toll-like receptor dimerization and subsequent activation of down-stream signalling pathways. Thus, P. aeruginosa ‘hijacks’ an endogenous anti-inflammatory peptide-based mechanism, thereby enabling modulation and circumvention of host responses.
Critical phenomena of emergent magnetic monopoles in a chiral magnet
Authors: Kanazawa N. , Nii Y. , Zhang X. -X. , Mishchenko A. S. , De Filippis G. , Kagawa F. , Iwasa Y. , Nagaosa N. , Tokura Y.
Published: May 2016

Second-order continuous phase transitions are characterized by symmetry breaking with order parameters. Topological orders of electrons, characterized by the topological index defined in momentum space, provide a distinct perspective for phase transitions, which are categorized as quantum phase transitions not being accompanied by symmetry breaking. However, there are still limited observations of counterparts in real space. Here we show a real-space topological phase transition in a chiral magnet MnGe, hosting a periodic array of hedgehog and antihedgehog topological spin singularities. This transition is driven by the pair annihilation of the hedgehogs and antihedgehogs acting as monopoles and antimonopoles of the emergent electromagnetic field. Observed anomalies in the magnetoresistivity and phonon softening are consistent with the theoretical prediction of critical phenomena associated with enhanced fluctuations of emergent field near the transition. This finding reveals a vital role of topology of the spins in strongly correlated systems.
A method for controlling the synthesis of stable twisted two-dimensional conjugated molecules
Authors: Li Yongjun , Jia Zhiyu , Xiao Shengqiang , Liu Huibiao , Li Yuliang
Published: May 2016

Thermodynamic stabilization (π-electron delocalization through effective conjugation) and kinetic stabilization (blocking the most-reactive sites) are important considerations when designing stable polycyclic aromatic hydrocarbons displaying tunable optoelectronic properties. Here, we demonstrate an efficient method for preparing a series of stable two-dimensional (2D) twisted dibenzoterrylene-acenes. We investigated their electronic structures and geometries in the ground state through various experiments assisted by calculations using density functional theory. We find that the length of the acene has a clear effect on the photophysical, electrochemical, and magnetic properties. These molecules exhibit tunable ground-state structures, in which a stable open-shell quintet tetraradical can be transferred to triplet diradicals. Such compounds are promising candidates for use in nonlinear optics, field effect transistors and organic spintronics; furthermore, they may enable broader applications of 2D small organic molecules in high-performance electronic and optical devices.
Solution structure of the isolated histone H2A-H2B heterodimer
Authors: Moriwaki Yoshihito , Yamane Tsutomu , Ohtomo Hideaki , Ikeguchi Mitsunori , Kurita Jun-ichi , Sato Masahiko , Nagadoi Aritaka , Shimojo Hideaki , Nishimura Yoshifumi
Published: May 2016

During chromatin-regulated processes, the histone H2A-H2B heterodimer functions dynamically in and out of the nucleosome. Although detailed crystal structures of nucleosomes have been established, that of the isolated full-length H2A-H2B heterodimer has remained elusive. Here, we have determined the solution structure of human H2A-H2B by NMR coupled with CS-Rosetta. H2A and H2B each contain a histone fold, comprising four α-helices and two β-strands (α1–β1–α2–β2–α3–αC), together with the long disordered N- and C-terminal H2A tails and the long N-terminal H2B tail. The N-terminal αN helix, C-terminal β3 strand and 310 helix of H2A observed in the H2A-H2B nucleosome structure are disordered in isolated H2A-H2B. In addition, the H2A α1 and H2B αC helices are not well fixed in the heterodimer and the H2A and H2B tails are not completely random coils. Comparison of hydrogen-deuterium exchange, fast hydrogen exchange and {1H}-15N hetero-nuclear NOE data with the CS-Rosetta structure indicates that there is some conformation in the H2A 310 helical and H2B Lys11 regions, while the repression domain of H2B (residues 27–34) exhibits an extended string-like structure. This first structure of the isolated H2A-H2B heterodimer provides insight into its dynamic functions in chromatin.
A novel WS2 nanowire-nanoflake hybrid material synthesized from WO3 nanowires in sulfur vapor
Authors: Asres Georgies Alene , Dombovari Aron , Sipola Teemu , Puskás Robert , Kukovecz Akos , Kónya Zoltán , Popov Alexey , Lin Jhih-Fong , Lorite Gabriela S. , Mohl Melinda , Toth Geza , Lloyd Spetz Anita , Kordas Krisztian
Published: May 2016

In this work, WS2 nanowire-nanoflake hybrids are synthesized by the sulfurization of hydrothermally grown WO3 nanowires. The influence of temperature on the formation of products is optimized to grow WS2 nanowires covered with nanoflakes. Current-voltage and resistance-temperature measurements carried out on random networks of the nanostructures show nonlinear characteristics and negative temperature coefficient of resistance indicating that the hybrids are of semiconducting nature. Bottom gated field effect transistor structures based on random networks of the hybrids show only minor modulation of the channel conductance upon applied gate voltage, which indicates poor electrical transport between the nanowires in the random films. On the other hand, the photo response of channel current holds promise for cost-efficient solution process fabrication of photodetector devices working in the visible spectral range.
CD23 can negatively regulate B-cell receptor signaling
Authors: Liu Chaohong , Richard Katharina , Wiggins Melvin , Zhu Xiaoping , Conrad Daniel H. , Song Wenxia
Published: May 2016

CD23 has been implicated as a negative regulator of IgE and IgG antibody responses. However, whether CD23 has any role in B-cell activation remains unclear. We examined the expression of CD23 in different subsets of peripheral B cells and the impact of CD23 expression on the early events of B-cell receptor (BCR) activation using CD23 knockout (KO) mice. We found that in addition to marginal zone B cells, mature follicular B cells significantly down regulate the surface expression level of CD23 after undergoing isotype switch and memory B-cell differentiation. Upon stimulation with membrane-associated antigen, CD23 KO causes significant increases in the area of B cells contacting the antigen-presenting membrane and the magnitude of BCR clustering. This enhanced cell spreading and BCR clustering is concurrent with increases in the levels of phosphorylation of tyrosine and Btk, as well as the levels of F-actin and phosphorylated Wiskott Aldrich syndrome protein, an actin nucleation promoting factor, in the contract zone of CD23 KO B cells. These results reveal a role of CD23 in the negative regulation of BCR signaling in the absence of IgE immune complex and suggest that CD23 down-regulates BCR signaling by influencing actin-mediated BCR clustering and B-cell morphological changes.
Enhanced adsorptive removal of p-nitrophenol from water by aluminum metal–organic framework/reduced graphene oxide composite
Authors: Wu Zhibin , Yuan Xingzhong , Zhong Hua , Wang Hou , Zeng Guangming , Chen Xiaohong , Wang Hui , zhang Lei , Shao Jianguang
Published: May 2016

In this study, the composite of aluminum metal–organic framework MIL-68(Al) and reduced graphene oxide (MA/RG) was synthesized via a one–step solvothermal method, and their performances for p–nitrophenol (PNP) adsorption from aqueous solution were systematically investigated. The introduction of reduced graphene oxide (RG) into MIL-68(Al) (MA) significantly changes the morphologies of the MA and increases the surface area. The MA/RG-15% prepared at RG-to-MA mass ratio of 15% shows a PNP uptake rate 64% and 123% higher than MIL-68(Al) and reduced graphene oxide (RG), respectively. The hydrogen bond and π – π dispersion were considered to be the major driving force for the spontaneous and endothermic adsorption process for PNP removal. The adsorption kinetics, which was controlled by film–diffusion and intra–particle diffusion, was greatly influenced by solution pH, ionic strength, temperature and initial PNP concentration. The adsorption kinetics and isotherms can be well delineated using pseudo–second–order and Langmuir equations, respectively. The presence of phenol or isomeric nitrophenols in the solution had minimal influence on PNP adsorption by reusable MA/RG composite.
Plasmon-enhanced Electrically Light-emitting from ZnO Nanorod Arrays/p-GaN Heterostructure Devices
Authors: Lu Junfeng , Shi Zengliang , Wang Yueyue , Lin Yi , Zhu Qiuxiang , Tian Zhengshan , Dai Jun , Wang Shufeng , Xu Chunxiang
Published: May 2016

Effective and bright light-emitting-diodes (LEDs) have attracted broad interests in fundamental research and industrial application, especially on short wavelength LEDs. In this paper, a well aligned ZnO nanorod arrays grown on the p-GaN substrate to form a heterostructured light-emitting diode and Al nanoparticles (NPs) were decorated to improve the electroluminescence performance. More than 30-folds enhancement of the electroluminescence intensity was obtained compared with the device without Al NPs decoration. The investigation on the stable and transient photoluminescence spectraof the ZnO nanorod arrays before and after Al NPs decoration demonstrated that the metal surface plasmon resonance coupling with excitons of ZnO leads to the enhancement of the internal quantum efficiency (IQE). Our results provide aneffective approach to design novel optoelectronic devices such as light-emitting diodes and plasmonic nanolasers.
Transcriptomic responses of the calanoid copepod Calanus finmarchicus to the saxitoxin producing dinoflagellate Alexandrium fundyense
Authors: Roncalli Vittoria , Cieslak Matthew C. , Lenz Petra H.
Published: May 2016

In the Gulf of Maine, the copepod Calanus finmarchicus co-occurs with the neurotoxin-producing dinoflagellate, Alexandrium fundyense. The copepod is resistant to this toxic alga, but little is known about other effects. Gene expression profiles were used to investigate the physiological response of females feeding for two and five days on a control diet or a diet containing either a low or a high dose of A. fundyense. The physiological responses to the two experimental diets were similar, but changed between the time points. At 5-days the response was characterized by down-regulated genes involved in energy metabolism. Detoxification was not a major component of the response. Instead, genes involved in digestion were consistently regulated, suggesting that food assimilation may have been affected. Thus, predicted increases in the frequency of blooms of A. fundyense could affect C. finmarchicus populations by changing the individuals’ energy budget and reducing their ability to build lipid reserves.
Spectral Camera based on Ghost Imaging via Sparsity Constraints
Authors: Liu Zhentao , Tan Shiyu , Wu Jianrong , Li Enrong , Shen Xia , Han Shensheng
Published: May 2016

The image information acquisition ability of a conventional camera is usually much lower than the Shannon Limit since it does not make use of the correlation between pixels of image data. Applying a random phase modulator to code the spectral images and combining with compressive sensing (CS) theory, a spectral camera based on true thermal light ghost imaging via sparsity constraints (GISC spectral camera) is proposed and demonstrated experimentally. GISC spectral camera can acquire the information at a rate significantly below the Nyquist rate and the resolution of the cells in the three-dimensional (3D) spectral images data-cube can be achieved with a two-dimensional (2D) detector in a single exposure. For the first time, GISC spectral camera opens the way of approaching the Shannon Limit determined by Information Theory in optical imaging instruments.
Ces3/TGH Deficiency Attenuates Steatohepatitis
Authors: Lian Jihong , Wei Enhui , Groenendyk Jody , Das Subhash K. , Hermansson Martin , Li Lena , Watts Russell , Thiesen Aducio , Oudit Gavin Y. , Michalak Marek , Lehner Richard
Published: May 2016

Nonalcoholic fatty liver disease (NAFLD) is the most common form of chronic liver disease in developed countries. NAFLD describes a wide range of liver pathologies from simple steatosis to nonalcoholic steatohepatitis (NASH) and cirrhosis. NASH is distinguished from simple steatosis by inflammation, cell death and fibrosis. In this study we found that mice lacking triacylglycerol hydrolase (TGH, also known as carboxylesterase 3 or carboxylesterase 1d) are protected from high-fat diet (HFD) - induced hepatic steatosis via decreased lipogenesis, increased fatty acid oxidation and improved hepatic insulin sensitivity. To examine the effect of the loss of TGH function on the more severe NAFLD form NASH, we ablated Tgh expression in two independent NASH mouse models, Pemt−/− mice fed HFD and Ldlr−/− mice fed high-fat, high-cholesterol Western-type diet (WTD). TGH deficiency reduced liver inflammation, oxidative stress and fibrosis in Pemt−/− mice. TGH deficiency also decreased NASH in Ldlr−/− mice. Collectively, these findings indicate that TGH deficiency attenuated both simple hepatic steatosis and irreversible NASH.
Geometric conductive filament confinement by nanotips for resistive switching of HfO2-RRAM devices with high performance
Authors: Niu Gang , Calka Pauline , Auf der Maur Matthias , Santoni Francesco , Guha Subhajit , Fraschke Mirko , Hamoumou Philippe , Gautier Brice , Perez Eduardo , Walczyk Christian , Wenger Christian , Di Carlo Aldo , Alff Lambert , Schroeder Thomas
Published: May 2016

Filament-type HfO2-based RRAM has been considered as one of the most promising candidates for future non-volatile memories. Further improvement of the stability, particularly at the “OFF” state, of such devices is mainly hindered by resistance variation induced by the uncontrolled oxygen vacancies distribution and filament growth in HfO2 films. We report highly stable endurance of TiN/Ti/HfO2/Si-tip RRAM devices using a CMOS compatible nanotip method. Simulations indicate that the nanotip bottom electrode provides a local confinement for the electrical field and ionic current density; thus a nano-confinement for the oxygen vacancy distribution and nano-filament location is created by this approach. Conductive atomic force microscopy measurements confirm that the filaments form only on the nanotip region. Resistance switching by using pulses shows highly stable endurance for both ON and OFF modes, thanks to the geometric confinement of the conductive path and filament only above the nanotip. This nano-engineering approach opens a new pathway to realize forming-free RRAM devices with improved stability and reliability.
Usp9x-deficiency disrupts the morphological development of the postnatal hippocampal dentate gyrus
Authors: Oishi Sabrina , Premarathne Susitha , Harvey Tracey J. , Iyer Swati , Dixon Chantelle , Alexander Suzanne , Burne Thomas H. J. , Wood Stephen A. , Piper Michael
Published: May 2016

Within the adult mammalian brain, neurogenesis persists within two main discrete locations, the subventricular zone lining the lateral ventricles and the hippocampal dentate gyrus. Neurogenesis within the adult dentate gyrus contributes to learning and memory and deficiencies in neurogenesis have been linked to cognitive decline. Neural stem cells within the adult dentate gyrus reside within the subgranular zone (SGZ) and proteins intrinsic to stem cells and factors within the niche microenvironment, are critical determinants for development and maintenance of this structure. Our understanding of the repertoire of these factors, however, remains limited. The deubiquitylating enzyme USP9X has recently emerged as a mediator of neural stem cell identity. Furthermore, mice lacking Usp9x exhibit a striking reduction in the overall size of the adult dentate gyrus. Here we reveal that the development of the postnatal SGZ is abnormal in mice lacking Usp9x. Usp9x conditional knockout mice exhibit a smaller hippocampus and shortened dentate gyrus blades from as early as P7. Moreover, the analysis of cellular populations within the dentate gyrus revealed reduced stem cell, neuroblast and neuronal numbers and abnormal neuroblast morphology. Collectively, these findings highlight the critical role played by USP9X in the normal morphological development of the postnatal dentate gyrus.
Evolution of Genome Size in Asexual Digital Organisms
Authors: Gupta Aditi , LaBar Thomas , Miyagi Miriam , Adami Christoph
Published: May 2016

Genome sizes have evolved to vary widely, from 250 bases in viroids to 670 billion bases in some amoebas. This remarkable variation in genome size is the outcome of complex interactions between various evolutionary factors such as mutation rate and population size. While comparative genomics has uncovered how some of these evolutionary factors influence genome size, we still do not understand what drives genome size evolution. Specifically, it is not clear how the primordial mutational processes of base substitutions, insertions, and deletions influence genome size evolution in asexual organisms. Here, we use digital evolution to investigate genome size evolution by tracking genome edits and their fitness effects in real time. In agreement with empirical data, we find that mutation rate is inversely correlated with genome size in asexual populations. We show that at low point mutation rate, insertions are significantly more beneficial than deletions, driving genome expansion and the acquisition of phenotypic complexity. Conversely, the high mutational load experienced at high mutation rates inhibits genome growth, forcing the genomes to compress their genetic information. Our analyses suggest that the inverse relationship between mutation rate and genome size is a result of the tradeoff between evolving phenotypic innovation and limiting the mutational load.
Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells
Authors: Mandal Ajeet , Mandal Swati , Park Myung Hee
Published: May 2016

The eukaryotic translation factor, eIF5A, is a translation factor essential for protein synthesis, cell growth and animal development. By use of a adenoviral eIF5A shRNA, we have achieved an effective depletion of eIF5A in HeLa cells and undertook in vivo comprehensive proteomic analyses to examine the effects of eIF5A depletion on the total proteome and to identify cellular pathways influenced by eIF5A. The proteome of HeLa cells transduced with eIF5A shRNA was compared with that of scramble shRNA-transduced counterpart by the iTRAQ method. We identified 972 proteins consistently detected in three iTRAQ experiments and 104 proteins with significantly altered levels (protein ratio ≥1.5 or ≤0.66, p-value ≤0.05) at 72 h and/or 96 h of Ad-eIF5A-shRNA transduction. The altered expression levels of key pathway proteins were validated by western blotting. Integration of functional ontology with expression data of the 104 proteins revealed specific biological processes that are prominently up- or down-regulated. Heatmap analysis and Cytoscape visualization of biological networks identified protein folding as the major cellular process affected by depletion of eIF5A. Our unbiased, quantitative, proteomic data demonstrate that the depletion of eIF5A leads to endoplasmic reticulum stress, an unfolded protein response and up-regulation of chaperone expression in HeLa cells.
Concurrent Formation of Carbon–Carbon Bonds and Functionalized Graphene by Oxidative Carbon-Hydrogen Coupling Reaction
Authors: Morioku Kumika , Morimoto Naoki , Takeuchi Yasuo , Nishina Yuta
Published: May 2016

Oxidative C–H coupling reactions were conducted using graphene oxide (GO) as an oxidant. GO showed high selectivity compared with commonly used oxidants such as (diacetoxyiodo) benzene and 2,3-dichloro-5,6-dicyano-p-benzoquinone. A mechanistic study revealed that radical species contributed to the reaction. After the oxidative coupling reaction, GO was reduced to form a material that shows electron conductivity and high specific capacitance. Therefore, this system could concurrently achieve two important reactions: C–C bond formationviaC–H transformation and production of functionalized graphene.
Awareness of Emotional Stimuli Determines the Behavioral Consequences of Amygdala Activation and Amygdala-Prefrontal Connectivity
Authors: Lapate R. C. , Rokers B. , Tromp D. P. M. , Orfali N. S. , Oler J. A. , Doran S. T. , Adluru N. , Alexander A. L. , Davidson R. J.
Published: May 2016

Conscious awareness of negative cues is thought to enhance emotion-regulatory capacity, but the neural mechanisms underlying this effect are unknown. Using continuous flash suppression (CFS) in the MRI scanner, we manipulated visual awareness of fearful faces during an affect misattribution paradigm, in which preferences for neutral objects can be biased by the valence of a previously presented stimulus. The amygdala responded to fearful faces independently of awareness. However, when awareness of fearful faces was prevented, individuals with greater amygdala responses displayed a negative bias toward unrelated novel neutral faces. In contrast, during the aware condition, inverse coupling between the amygdala and prefrontal cortex reduced this bias, particularly among individuals with higher structural connectivity in the major white matter pathway connecting the prefrontal cortex and amygdala. Collectively, these results indicate that awareness promotes the function of a critical emotion-regulatory network targeting the amygdala, providing a mechanistic account for the role of awareness in emotion regulation.
Local microstructure evolution at shear bands in metallic glasses with nanoscale phase separation
Authors: He Jie , Kaban Ivan , Mattern Norbert , Song Kaikai , Sun Baoan , Zhao Jiuzhou , Kim Do Hyang , Eckert Jürgen , Greer A. Lindsay
Published: May 2016

At room temperature, plastic flow of metallic glasses (MGs) is sharply localized in shear bands, which are a key feature of the plastic deformation in MGs. Despite their clear importance and decades of study, the conditions for formation of shear bands, their structural evolution and multiplication mechanism are still under debate. In this work, we investigate the local conditions at shear bands in new phase-separated bulk MGs containing glassy nanospheres and exhibiting exceptional plasticity under compression. It is found that the glassy nanospheres within the shear band dissolve through mechanical mixing driven by the sharp strain localization there, while those nearby in the matrix coarsen by Ostwald ripening due to the increased atomic mobility. The experimental evidence demonstrates that there exists an affected zone around the shear band. This zone may arise from low-strain plastic deformation in the matrix between the bands. These results suggest that measured property changes originate not only from the shear bands themselves, but also from the affected zones in the adjacent matrix. This work sheds light on direct visualization of deformation-related effects, in particular increased atomic mobility, in the region around shear bands.
The functional role of time compression
Authors: Zimmermann Eckart , Derichs Christina , Fink Gereon R.
Published: May 2016

Multisensory integration provides continuous and stable perception from separate sensory inputs. Here, we investigated the functional role of temporal binding between the visual and the tactile senses. To this end we used the paradigm of compression that induces shifts in time when probe stimuli are degraded, e.g., by a visual mask (Zimmermann et al. 2014). Subjects had to estimate the duration of temporal intervals of 500 ms defined by a tactile and a visual, masked stimulus. We observed a strong (~100 ms) underestimation of the temporal interval when the stimuli from both senses appeared to occur at the same position in space. In contrast, when the positions of the visual and tactile stimuli were spatially separate, interval perception was almost veridical. Temporal compression furthermore depended on the correspondence of probe features and was absent when the orientation of the tactile and visual probes was incongruent. An additional experiment revealed that temporal compression also occurs when objects were presented outside the attentional focus. In conclusion, these data support a role for spatiotemporal binding in temporal compression, which is at least in part selective for object features.