bluuebunny/crossref_metadata_2025 huggingface dataset:
Lipid-Lowering Efficacy of the Capsaicin in Patients With Metabolic Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Jiang Zhonghui , Qu Hua , Lin Gongyu , Shi Dazhuo , Chen Keji , Gao Zhuye
Published: March 2022

Background

Patients with metabolic syndrome (MetS) have increased cardiovascular risk. Capsaicin (CAP) has been shown to reduce lipids, but efficacy for patients with MetS is unknown.

Methods

A systematic review was performed according to PRISMA guidelines, to compare the effects of CAP against a placebo. Differences in the weight mean difference (WMD) with 95% confidence intervals (95% CI) were then pooled using a random effects model.

Results

Nine randomized controlled trials including 461 patients were identified in the overall analysis. CAP significantly decreased total cholesterol (TC) (WMD = −0.48, 95% CI: −0.63 to −0.34, I2= 0.00%) and low-density lipoprotein cholesterol (LDL-C) (WMD = −0.23, 95% CI: −0.45 to −0.02, I2 = 68.27%) among patients with MetS. No significant effects of CAP were found on triglycerides (TG) or high-density lipoprotein cholesterol (HDL-C) (WMD = −0.40, 95% CI: −1.50 to 0.71, I2 = 98.32%; WMD = −0.08, 95% CI: −0.21 to 0.04, I2 = 86.06%). Subgroup analyses indicated that sex and intervention period were sources of heterogeneity. The results revealed that CAP decreased TG levels in women (WMD = −0.59, 95% CI: −1.07 to −0.10) and intervention period <12 weeks (WMD = −0.65; 95% CI: −1.10 to −0.20). And there was no potential publication bias according to funnel plot, Begg' test and Egger regression test.

Conclusions

CAP supplementation is a promising approach to decreasing TC and LCL-C levels in patients with MetS. However, short-term (<12 weeks) use of CAP in women may also reduce TG levels.

Systematic Review Registration

Identifier: CRD42021228032.
Development and Validation of a Novel Prognostic Model for Lower-Grade Glioma Based on Enhancer RNA-Regulated Prognostic Genes
Authors: Tian Wei , Yan Guangcan , Chen Kegong , Han Xinhao , Zhang Wei , Sun Lin , Zhang Qi , Zhang Yafeng , Li Yan , Liu Meina , Zhang Qiuju
Published: March 2022

Enhancer RNAs (eRNAs) are present specifically in tumors, where they affect the expression of eRNA-regulated genes (ERGs). Owing to this characteristic, ERGs were hypothesized to improve prognosis of overall survival in heterogeneous low-grade and intermediate-grade gliomas. This study aimed to construct and validate an ERG prognostic tool to facilitate clinical management, and offer more effective diagnostic and therapeutic biomarkers for glioma. Survival-related eRNAs were identified, and their ERGs were selected based on eRNA and target gene information. The ERG prognostic model was constructed and validated using internal and external validation cohorts. Finally, biological differences related to the ERG signature were analysed to explore the potential mechanisms influencing survival outcomes. Thirteen ERGs were identified and used to build an ERG risk signature, which included five super-enhancer RNA (seRNA)-regulated genes and five LGG-specific eRNA-regulated genes. The prognostic nomogram established based on combining the ERG score, age, and sex was evaluated by calibration curves, clinical utility, Harrell’s concordance index (0.86; 95% CI: 0.83-0.90), and time-dependent receiver operator characteristic curves. We also explored potential immune-related mechanisms that might cause variation in survival. The established prognostic model displayed high validity and robustness. Several immune-related genes regulated by seRNAs or specific eRNAs were identified, indicating that these transcripts or their genes were potential targets for improving immunotherapeutic/therapeutic outcomes. The functions of an important specific eRNA-regulated gene (USP28) were validated in robust vitro experiments. In addition, the ERG risk signature was significantly associated with the immune microenvironment and other immune-related features.
Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells
Authors: Zhou Lijie , Li Hongyu , Cai Hongshi , Liu Wenhui , Pan Enjiu , Yu Dongsheng , He Shuai
Published: March 2022

The strong invasive and metastatic abilities of oral squamous cell carcinoma (OSCC) cells in the early stage are the main reason for its poor prognosis. The early diagnosis and treatment of OSCC may reduce the metastasis rate and improve the survival rate. The aim of this study was to explore candidate biomarkers related to the prognosis and progression of OSCC. We performed weighted gene coexpression network analysis to identify key modules and genes associated with OSCC and intersected the differentially expressed genes (DEGs) in The Cancer Genome Atlas (TCGA)-OSCC and GSE30784 datasets. Next, we performed survival analysis and immunohistochemistry to screen and validate the hub gene insulin-like growth factor 2 (IGF2) mRNA binding protein 2 IGF2BP2. We also used TCGA pan-cancer data to verify that IGF2BP2 was expressed at high levels in a variety of cancers and was related to a poor prognosis in patients. Furthermore, we divided patients with OSCC into high and low expression groups based on the median expression level of IGF2BP2. Gene set enrichment analysis (GSEA) showed that IGF2BP2 led to a poor prognosis in OSCC by affecting cancer-related (epithelial-mesenchymal transition, glycolysis, cell cycle, etc.) and immune-related biological functions and pathways. Single-sample GSEA (ssGSEA), CIBERSORT, and xCell algorithms helped reveal that high IGF2BP2 expression was accompanied by a significant reduction in the immune score, stromal score, and microenvironment score and a decrease in the number of infiltrating CD8+ T cells in OSCC. In addition, silencing IGF2BP2 suppressed the proliferation, migration, and invasion of OSCC cells. In general, IGF2BP2 is a potential biomarker for the progression, immunotherapy response, and prognosis of OSCC.
The Chaperone Protein GRP78 Promotes Survival and Migration of Head and Neck Cancer After Direct Radiation Exposure and Extracellular Vesicle-Transfer
Authors: Schneider Michael , Winkler Klaudia , Kell Rosemarie , Pfaffl Michael W. , Atkinson Michael J. , Moertl Simone
Published: March 2022

Background and Purpose

Increased levels of the chaperone protein GRP78 have been implicated in poorer outcomes of cancer therapy. We have therefore explored the functional connection between the expression of GRP78 and the development of radioresistance and metastatic behavior in HNSCC.

Material and Methods

The association between gene expression of GRP78 and survival in HNSCC patients was examined using the TCGA database. The influence of ionizing radiation on the GRP78 levels in HNSCC cell lines, their secreted extracellular vesicles (EV) and non-irradiated EV-recipient cells was investigated by Western Blot and FACS. The consequences of chemical inhibition or experimental overexpression of GRP78 on radioresistance and migration of HNSCC cells were analyzed by clonogenic survival and gap closure assays.

Results

Elevated levels of GRP78 RNA in HNSCC correlated with poorer overall survival. Radiation increased GRP78 protein expression on the surface of HNSCC cell lines. Experimental overexpression of GRP78 increased both radioresistance and migratory potential. Chemical inhibition of GRP78 impaired cell migration. EVs were identified as a potential source of increased GRP78 content as elevated levels of surface GRP78 were found in EVs released by irradiated cells. These vesicles transferred GRP78 to non-irradiated recipient cells during co-cultivation.

Conclusions

We have identified the chaperone protein GRP78 as a potential driver of increased radioresistance and motility in HNSCC. The uptake of GRP78-rich EVs originating from irradiated cells may contribute to a poorer prognosis through bystander effects mediated by the transfer of GRP78 to non-irradiated cells. Therefore, we consider the chaperone protein GRP78 to be an attractive target for improving radiotherapy strategies.
Systematic Review of Olaparib in the Treatment of Recurrent Platinum Sensitive Ovarian Cancer
Authors: Chen Qian , Li Xiaoli , Zhang Zhen , Wu Tong
Published: March 2022

Objective

To systematically evaluate the efficacy and safety of olaparib in the treatment of recurrent platinum-sensitive ovarian cancer.

Methods

The Cochrane Library, PubMed, Chinese Biomedical Literature Database, CNKI, VIP Database, Wanfang Science and Technology Database were searched for randomized controlled trials (RCTs) of olaparib in the treatment of recurrent platinum-sensitive ovarian cancer from the establishment of each database to January 2022. Two reviewers independently evaluated the quality of the literature, extracted the data, and cross-checked the methodological quality. Meta-analysis was performed using RevMan 5.4 software.

Results

A total of 7 RCTs were included, including 2406 patients, There were 1497 patients in treatment groups and 909 patients in the control group. Meta-analysis results showed that in terms of effectiveness, the overall survival time of patients in the olaparib group [HR=1.24, 95%CI(1.06, 1.45), P=0.006]; in terms of safety, for all grades of adverse events (including nausea, fatigue, vomiting, diarrhea, abdominal pain, and headache), [HR=1.54, 95%CI(1.38, 1.71), P=0.0002], for grade 3 or higher adverse events (including nausea, fatigue, vomiting, diarrhea, abdominal pain, and headache), [HR=2.13, 95%CI(1.61, 2.81), P=0.003], there were significant differences compared with the control group, suggesting that the risk of adverse reactions in the experimental group was higher than that in the control group. Subgroup analysis showed that only abdominal pain, headache and vomiting were not statistically significant, and other adverse reactions were statistically significant.

Conclusion

Based on the existing clinical evidence, olaparib in the treatment of recurrent platinum-sensitive ovarian cancer has a longer overall survival than the control group. It is an ideal regimen, but the incidence of adverse reactions is high.
The Clinical Outcomes, Prognostic Factors and Nomogram Models for Primary Lung Cancer Patients Treated With Stereotactic Body Radiation Therapy
Authors: Luo Li-Mei , Wang Ying , Lin Pei-Xian , Su Chuang-Huang , Huang Bao-Tian
Published: March 2022

Purpose

Stereotactic body radiation therapy (SBRT) is a standard treatment for early primary lung cancer patients. However, there are few simple models for predicting the clinical outcomes of these patients. Our study analyzed the clinical outcomes, identified the prognostic factors, and developed prediction nomogram models for these patients.

Materials and Methods

We retrospectively analyzed 114 patients with primary lung cancer treated with SBRT from 2012 to 2020 at our institutions and assessed patient’s clinical outcomes and levels of toxicity. Kaplan–Meier analysis with a log-rank test was used to generate the survival curve. The cut-off values of continuous factors were calculated with the X-tile tool. Potential independent prognostic factors for clinical outcomes were explored using cox regression analysis. Nomograms for clinical outcomes prediction were established with identified factors and assessed by calibration curves.

Results

The median overall survival (OS) was 40.6 months, with 3-year OS, local recurrence free survival (LRFS), distant disease-free survival (DDFS) and progression free survival (PFS) of 56.3%, 61.3%, 72.9% and 35.8%, respectively, with grade 3 or higher toxicity rate of 7%. The cox regression analysis revealed that the clinical stage, immobilization device, and the prescription dose covering 95% of the target area (D95) were independent prognostic factors associated with OS. Moreover, the clinical stage, and immobilization device were independent prognostic factors of LRFS and PFS. The smoking status, hemoglobin (Hb) and immobilization device were significant prognostic factors for DDFS. The nomograms and calibration curves incorporating the above factors indicated good predictive accuracy.

Conclusions

SBRT is effective and safe for primary lung cancer. The prognostic factors associated with OS, LRFS, DDFS and PFS are proposed, and the nomograms we proposed are suitable for clinical outcomes prediction.
New Insights and Advances in Pathogenesis and Treatment of Very Early Onset Inflammatory Bowel Disease
Authors: Li Qi-Qi , Zhang Hui-Hong , Dai Shi-Xue
Published: March 2022

Very early onset inflammatory bowel disease (VEO-IBD) is characterized by multifactorial chronic recurrent intestinal inflammation. Compared with elderly patients, those with VEO-IBD have a more serious condition, not responsive to conventional treatments, with a poor prognosis. Recent studies found that genetic and immunologic abnormalities are closely related to VEO-IBD. Intestinal immune homeostasis monogenic defects (IIHMDs) are changed through various mechanisms. Recent studies have also revealed that abnormalities in genes and immune molecular mechanisms are closely related to VEO-IBD. IIHMDs change through various mechanisms. Epigenetic factors can mediate the interaction between the environment and genome, and genetic factors and immune molecules may be involved in the pathogenesis of the environment and gut microbiota. These discoveries will provide new directions and ideas for the treatment of VEO-IBD.
Associations Between Parental Occupational Balance, Subjective Health, and Clinical Characteristics of VLBW Infants
Authors: Dür Mona , Röschel Anna , Oberleitner-Leeb Christiane , Herrmanns Verena , Pichler-Stachl Elisabeth , Mattner Barbara , Pernter Silvia-Desiree , Wald Martin , Urlesberger Berndt , Kurz Herbert , Frischer Thomas , Zwiauer Karl , Matter Inu Sarah , Berger Angelika
Published: March 2022

Objective

Very low birthweight (VLBW) infants have an increased risk of mortality and frequently suffer from complications, which affects parental occupational balance. Occupational balance is the satisfaction with one's meaningful activities, which include everyday activities that people need to, want to, and are expected to do. In contrast to work-life balance, the construct of occupational balance addresses different activities equally and it applies to all persons, regardless of whether they are working or not. Parental occupational balance might be related to parents' and VLBW infants' health. Therefore, the objective of this study was to investigate associations between parental occupational balance, subjective health, and clinical characteristics of VLBW infants.

Methods

A cross-sectional multicenter study was conducted in six Austrian neonatal intensive care units. Occupational balance and subjective health of parents of VLBW infants were assessed with six self-reported questionnaires. The following clinical characteristics of VLBW infants were extracted from medical records: gestational age, birthweight, Apgar scores, Clinical Risk for Babies II Score, and complications of prematurity. Spearman's rank coefficients were calculated.

Results

In total, 270 parents, 168 (62%) female and their VLBW infants, 120 (44%) female, were included in this study. Parents' mean age was 33.7 (±6.0) years, mean gestational age of VLBW infants was 27 + 3 (±2) weeks. Associations between parental occupational balance, subjective health, and clinical characteristics of VLBW infants were identified (rs = 0.13 – 0.56; p ≤ 0.05), such as the correlation between occupational areas (rs = 0.22, p ≤ 0.01), occupational characteristics (rs = 0.17, p = 0.01), and occupational resilience (rs = 0.18, p ≤ 0.01) with bronchopulmonary dysplasia of VLBW infants.

Conclusion

Occupational balance is associated with parents' and VLBW infants' health. Interventions to strengthen parental occupational balance might increase parental health and thereby also improve health and developmental outcomes of their VLBW infants.
Overview of Cannabis including Kampo Medicine and Therapy for Treatment of Dementia: A Review
Authors: Wenger Tibor , Watanabe Kazuhito , Sasaki Yui , Kanazawa Keiko , Shimizu Koichi , Sirikantaramas Supaart , Shoyama Yoshinari , Taura Futoshi , Morimoto Satoshi , Shoyama Yukihiro
Published: March 2022

Cannabis sativa L. is an annual herb oldest cultivated plants as a source of fiber since about 5000 B.C. On the other hand, the cannabis flower and seed are listed in Shennong’s classic Materia Medica approximately 2000 years ago. The formulas prescribed with cannabis in Kampo medicine have been summarized. Cannabidiol (CBD) and tetrahydrocannabinol (THC) are the major neurological and psychiatric cannabinoids, and develop to drugs. It becomes evident that the therapeutic CBD and/or THC are the important candidate of anti-dementia drugs having different mechanism for Alzheimer’s patients. Two receptors and endocannabinoids are also discussed for underlying mechanism of action. In order to promote the breeding of cannabis plant containing higher concentration of target cannabinoid the biosynthetic enzymes were isolated, cloning and the tertiary structure of THCA synthase determined by x-ray analysis resulting in the possibility of molecular breeding for cannabinoids.
Possible Mechanisms Underlying the Effects of Glucagon-Like Peptide-1 Receptor Agonist on Cocaine Use Disorder
Authors: Zhu Changliang , Li Hailiang , Kong Xuerui , Wang Yezhong , Sun Tao , Wang Feng
Published: March 2022

Cocaine use disorder (CUD) is a major public health challenge with a high relapse rate and lack of effective pharmacotherapies; therefore, there is a substantial need to identify novel medications to treat this epidemic. Since the advent of glucagon-like peptide-1 (GLP-1) receptors (GLP-1Rs) agonists (GLP-1RAs), their potential has been extensively explored and expanded. In this review, we first summarized the biological effects of GLP-1, GLP-1Rs, and GLP-1RAs. Subsequently, the recent literature examining the behavioral effects and the possible pharmacological mechanisms of GLP-1RAs on CUD was reviewed. Increasing preclinical evidence suggests that GLP-1RAs are promising in regulating dopamine release, dopamine transporter (DAT) surface expression and function, mesolimbic reward system and GABAergic neurons, and maladaptive behaviors in animal models of self-administration and conditioned place preference. In addition, the emerging role of GLP-1RAs in inhibiting inflammatory cytokines was reported. These findings indicate that GLP-1RAs perform essential functions in the modulation of cocaine-seeking and cocaine-taking behaviors likely through multifaceted mechanisms. Although the current preclinical evidence provides convincing evidence to support GLP-1RA as a promising pharmacotherapy for CUD, other questions concerning clinical availability, impact and specific mechanisms remain to be addressed in further studies.
Lonafarnib Inhibits Farnesyltransferase via Suppressing ERK Signaling Pathway to Prevent Osteoclastogenesis in Titanium Particle-Induced Osteolysis
Authors: Huang Linke , Chen Weiwei , Wei Linhua , Su Yuangang , Liang Jiamin , Lian Haoyu , Wang Hui , Long Feng , Yang Fan , Gao Shiyao , Tan Zhen , Xu Jiake , Zhao Jinmin , Liu Qian
Published: March 2022

Wear debris after total joint arthroplasty can attract the recruitment of macrophages, which release pro-inflammatory substances, triggering the activation of osteoclasts, thereby leading to periprosthetic osteolysis (PPOL) and aseptic loosening. However, the development of pharmacological strategies targeting osteoclasts to prevent periprosthetic osteolysis has not been fruitful. In this study, we worked toward researching the effects and mechanisms of a farnesyltransferase (FTase) inhibitor Lonafarnib (Lon) on receptor activator of nuclear factor κB (NF-κB) ligand (RANKL)-induced osteoclastogenesis and bone resorption, as well as the impacts of Lon on titanium particle-induced osteolysis. To investigate the impacts of Lon on bone resorption and osteoclastogenesis in vitro, bone marrow macrophages were incubated and stimulated with RANKL and macrophage colony-stimulating factor (M-CSF). The influence of Lon on osteolysis prevention in vivo was examined utilizing a titanium particle-induced mouse calvarial osteolysis model. The osteoclast-relevant genes expression was explored by real-time quantitative PCR. Immunofluorescence was used to detect intracellular localization of nuclear factor of activated T cells 1 (NFATc1). SiRNA silence assay was applied to examine the influence of FTase on osteoclasts activation. Related signaling pathways, including NFATc1 signaling, NF-κB, mitogen-activated protein kinases pathways were identified by western blot assay. Lon was illustrated to suppress bone resorptive function and osteoclastogenesis in vitro, and it also reduced the production of pro-inflammatory substances and prevented titanium particle-induced osteolysis in vivo. Lon decreased the expression of osteoclast-relevant genes and suppressed NFATc1 nuclear translocation and auto-amplification. Mechanistically, Lon dampened FTase, and inhibition of FTase reduced osteoclast formation by suppressing ERK signaling. Lon is a promising treatment option for osteoclast-related osteolysis diseases including periprosthetic osteolysis by targeted inhibition of FTase through suppressing ERK signaling.
Target Characteristics Used in Laser-Plasma Acceleration of Protons Based on the TNSA Mechanism
Authors: Măgureanu A. , Dincă L. , Jalbă C. , Andrei R. F. , Burducea I. , Ghiţă D. G. , Nastasa V. , Gugiu M. , Asavei T. , Budrigă O. , Ticoş D. , Crăciun V. , Diaconescu B. , Ticoş C. M.
Published: March 2022

The target normal sheath acceleration is a robust mechanism for proton and ion acceleration from solid targets when irradiated by a high power laser. Since its discovery extensive studies have been carried out to enhance the acceleration process either by optimizing the laser pulse delivered onto the target or by utilizing targets with particular features. Targets with different morphologies such as the geometrical shape (thin foil, cone, spherical, foam-like, etc.), with different structures (multi-layer, nano- or micro-structured with periodic striations, rods, pillars, holes, etc.) and made of different materials (metals, plastics, etc.) have been proposed and utilized. Here we review some recent experiments and characterize from the target point of view the generation of protons with the highest energy.
Bifurcation and Chaos Analysis of Gear System With Clearance Under Different Load Conditions
Authors: Wang Jungang , Shan Zheng’ang , Chen Sheng
Published: March 2022

In the transmission process of gear system, the change of load will make the system in different states of motion, which affects the transmission efficiency of gear system. It is important to investigate the nonlinear dynamic characteristics of gear system under different load states. Using straight cylindrical gears as the object of study, the concentrated mass method is used to establish a dynamic model that takes into account nonlinear factors such as tooth side clearance, time-varying meshing stiffness and transmission errors. The differential equations of the system are solved by the Longe-Kutta method to obtain the bifurcation diagram, the maximum Lyapunov exponent diagram and the phase plane diagram of the gear system to analyze the effect of the meshing damping ratio on the dynamic characteristics of the system under different load states. The results show that the influence of the engagement damping ratio on the dynamic characteristics of the system is greater under light load conditions, showing different states of motion as the engagement damping ratio gradually increases. Under heavy load conditions, the effect of the engagement damping ratio on the dynamic characteristics of the system is small. Appropriately increasing the mesh damping ratio is beneficial to the gear system to avoid the chaotic zone and maintain a stable cyclic motion state. The results of the study provide a reference for the design of gear systems with variable loads.
The Opioid System in Rainbow Trout Telencephalon Is Probably Involved in the Hedonic Regulation of Food Intake
Authors: Díaz-Rúa Adrián , Chivite Mauro , Comesaña Sara , Conde-Sieira Marta , Soengas José L.
Published: March 2022

We hypothesize that opioids are involved in the regulation of food intake in fish through homeostatic and hedonic mechanisms. Therefore, we evaluated in rainbow trout (Oncorhynchus mykiss) hypothalamus and telencephalon changes in precursors, endogenous ligands and receptors of the opioid system under different situations aimed to induce changes in the homeostatic (through fasted/fed/refed fish) and hedonic (through feeding fish a control or a palatable high-fat diet) regulation of food intake. No major changes occurred in parameters assessed related with the nutritional condition of fish (fasted/fed/refed), allowing us to suggest that the opioid system seems not to have an important role in the homeostatic regulation of food intake in rainbow trout. The responses observed in telencephalon of rainbow trout fed the palatable high-fat diet included a decrease in mRNA abundance of the opioid precursor penka, in a way similar to that known in mammals, and increased mRNA abundance of the opioid receptors oprd1 and oprk1 supporting a role for telencephalic opioid system in the hedonic regulation of food intake in fish.
Empagliflozin Inhibits Hepatic Gluconeogenesis and Increases Glycogen Synthesis by AMPK/CREB/GSK3β Signalling Pathway
Authors: Yu Xiaochen , Meng Ziyu , Fang Ting , Liu Xiaohuan , Cheng Ying , Xu Linxin , Liu Xiangyang , Li Xiaoyu , Xue Mei , Li Ting , Sun Bei , Chen Liming
Published: March 2022

Increases in glucose production and decreases in hepatic glycogen storage induce glucose metabolic abnormalities in type 2 diabetes (T2DM). Empagliflozin, a sodium-dependent glucose transporter 2 (SGLT2) inhibitor, is an effective hypoglycemic drug; however, the effects of empagliflozin on hepatic gluconeogenesis and glycogenesis are still unclear. In this study, we investigated the effects and mechanisms of empagliflozin on hepatic gluconeogenesis and glycogenesis in vivo and in vitro. Empagliflozin was administered via gavage to db/db mice for 8 weeks, and human hepatocyte HL7702 cells were treated with empagliflozin after palmitic acid (PA) stimulation. Compared with the control db/db mice, empagliflozin-treated mice showed a significant reduction in urine glucose levels, blood glucose levels, body weight and intraperitoneal glucose tolerance test (IPGTT) blood glucose levels. Moreover, the expression levels and activities of key gluconeogenesis enzymes PEPCK and G6Pase were dramatically reduced in the empagliflozin-treated mice, and the protein expression levels of AMPK/CREB/GSK3β signalling pathway-related molecules were significantly changed. In HL7702 cells, empagliflozin ameliorated glucose production and PEPCK and G6Pase expression and activity. Empagliflozin could also prevent the decreases in glycogen content and regulate the protein expression levels of AMPK/CREB/GSK3β signalling pathway-related molecules. Then, we selected the AMPK agonist AICAR and inhibitor compound C to further verify the effects of the AMPK signalling pathway on hepatic gluconeogenesis and glycogen synthesis. The results of the 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AIACR) intervention in HL7702 cells were consistent with those of empagliflozin treatment, and the effects of empagliflozin were abolished by compound C. In summary, empagliflozin could maintain glucose homoeostasis by reducing gluconeogenesis and increasing glycogenesis through the AMPK/CREB/GSK3β signalling pathway.
Stigmatic Transcriptome Analysis of Self-Incompatible and Compatible Pollination in Corylus heterophylla Fisch. × Corylus avellana L.
Authors: Hou Sihao , Zhao Tiantian , Yang Zhen , Liang Lisong , Ma Wenxu , Wang Guixi , Ma Qinghua
Published: March 2022

Self-incompatibility (SI) protects plants from inbreeding depression due to self-pollination and promotes the outcrossing process to maintain a high degree of heterozygosity during evolution. Corylus is an important woody oil and nut species that shows sporophytic SI (SSI). Yet the molecular mechanism of SI in Corylus remains largely unknown. Here we conducted self- (“Dawei” × “Dawei”) and cross-pollination (“Dawei” × “Liaozhen No. 7”) experiments and then performed an RNA-Seq analysis to investigate the mechanism of pollen–stigma interactions and identify those genes that may be responsible for SSI in Corylus. We uncovered 19,163 up- and 13,314 downregulated genes from the comparison of different pollination treatments. These differentially expressed genes (DEGs) were significantly enriched in plant–pathogen interaction, plant hormone signal transduction, and MAPK signaling pathway–plant. We found many notable genes potentially involved in pollen–stigma interactions and SSI mechanisms, including genes encoding receptor-like protein kinases (RLK), calcium-related genes, disease-resistance genes, and WRKY transcription factors. Four upregulated and five downregulated DEGs were consistently identified in those comparison groups involving self-incompatible pollination, suggesting they had important roles in pollen–pistil interactions. We further identified the S-locus region of the Corylus heterophylla genome based on molecular marker location. This predicted S-locus contains 38 genes, of which 8 share the same functional annotation as the S-locus genes of Corylus avellana: two PIX7 homologous genes (EVM0002129 and EVM0025536), three MIK2 homologous genes (EVM0002422, EVM0005666, and EVM0009820), one aldose 1-epimerase (EVM0002095), one 3-dehydroquinate synthase II (EVM0021283), and one At3g28850 homologous gene (EVM0016149). By characterizing the pistil process during the early postpollination phase via transcriptomic analysis, this study provides new knowledge and lays the foundation for subsequent analyses of pollen-pistil interactions.
A High-Quality Reference Genome Sequence and Genetic Transformation System of Aralia elata
Authors: Liu Wenxuan , Guo Wenhua , Chen Song , Xu Honghao , Zhao Yue , Chen Su , You Xiangling
Published: March 2022

Aralia elata is a perennial woody plant of the genus Aralia in the family Araliaceae. It is rich in saponins and therefore has a wide range of pharmacological effects. Here, we report a high-quality reference genome of A. elata, with a genome size of 1.21 Gb and a contig N50 of 51.34 Mb, produced by PacBio HiFi sequencing technology. This is the first genome assembly for the genus Aralia. Through genome evolutionary analysis, we explored the phylogeny and whole genome duplication (WGD) events in the A. elata genome. The results indicated that a recent WGD event occurred in the A. elata genome. Estimation of the divergence times indicated that the WGD may be shared by Araliaceae. By analyzing the genome sequence of A. elata and combining the transcriptome data from three tissues, we discovered important genes related to triterpene saponins biosynthesis. Furthermore, based on the embryonic callus induction system of A. elata established in our laboratory, we set up the genetic transformation system of this plant. The genomic resources and genetic transformation system obtained in this study provide insights into A. elata and lays the foundation for further exploration of the A. elata regulatory mechanism.
Why and How Did Narrative Fictions Evolve? Fictions as Entertainment Technologies
Authors: Dubourg Edgar , Baumard Nicolas
Published: March 2022

Narrative fictions have surely become the single most widespread source of entertainment in the world. In their free time, humans read novels and comics, watch movies and TV series, and play video games: they consume stories that they know to be false. Such behaviors are expanding at lightning speed in modern societies. Yet, the question of the origin of fictions has been an evolutionary puzzle for decades: Are fictions biological adaptations, or the by-products of cognitive mechanisms that evolved for another purpose? The absence of any consensus in cognitive science has made it difficult to explain how narrative fictions evolve culturally. We argue that current conflicting hypotheses are partly wrong, and partly right: narrative fictions areby-productsof the human mind, because they obviously co-opt some pre-existing cognitive preferences and mechanisms, such as our interest for social information, and our abilities to do mindreading and to imagine counterfactuals. But humans reap some fitness benefits from producing and consuming such appealing cultural items, making fictionsadaptive. To reconcile these two views, we put forward the hypothesis that narrative fictions are best seen asentertainment technologiesthat is, as items crafted by some people for the proximate goal to grab the attention of other people, and with the ultimate goal to fulfill other evolutionary-relevant functions that become easier once other people’s attention is caught. This hypothesis explains why fictions are filled with exaggerated and entertaining stimuli, why they fit so well the changing preferences of the audience they target, and why producers constantly make their fictions more attractive as time goes by, in a cumulative manner.
Understanding EFL Teacher Engagement in TDTs’ Collaborative Curriculum Design: A Chinese Case Study From the Activity Theory Perspective
Authors: Wu Zhonghua , Li Jian , Cheng Le
Published: March 2022

While collaborative curriculum design (CCD) has gained increasing attention in the field of education, there is scant research as to how EFL teachers implement it in authentic design contexts. The present case study places particular emphasis on teacher activities during collaborative EFL curriculum development in China. The researchers adopt Activity Theory as an analytical tool to understand the relationships between EFL teachers, reform goals, and various aspects of the sociocultural context in which CCD is advocated, highlighting the pivotal role that mediation of knowledge/experience plays in the teacher learning community. Research findings indicate that the TDTs’ collaborative process is a socially constructed and culturally mediated activity that promotes EFL teacher development. Moreover, in-service teachers are prone to take challenges through educational reforms out of teacher motives and external stimuli. Limitations of the CCD model are also discussed to shed light on future research.
Gender Differences in Burnout Among Endocrinologists in China
Authors: Wang Jing , Zhang Lufa , Jiang Feng , Liu Yuanli , Wang Mingxiao , Wu Yinuo , Tang Yi-Lang
Published: March 2022

Objective

To survey the prevalence of burnout in a national sample of endocrinologists in China and to examine its correlates, with a special focus on gender differences.

Methods

An anonymous online survey was conducted among endocrinologists in 31 provincial government-owned “People’s Hospitals” of each province in mainland China. Demographic and work-related factors were collected from participants. The Maslach Burnout Inventory-Human Services Survey (MBI-HSS) was used to assess burnout, including emotional exhaustion (EE), depersonalization (DP), and reduced personal accomplishment (PA).

Results

A total of 711 endocrinologists (72.1% were female and mean age was 39.63 ± 8.51 years old) completed the survey. Burnout was reported by 32.8% of the participants. There were no significant gender differences in the overall prevalence of burnout or EE, DP, and PA (all p > 0.05). A multi-level linear regression revealed: (1) In male participants, PA was significantly associated with age (β = 0.03, p = 0.003), DP was inversely associated with age (β = −0.06, p = 0.005), EE was significantly associated with shorter sleep duration (β = −0.25, p = 0.006), and longer work hours (β = 0.01, p = 0.016). (2) In females, PA was significantly associated with age (β = 0.01, p = 0.038), EE and DP were both significantly associated with shorter sleep duration (β = −0.19, p = 0.001; and β = −0.15, p = 0.011, respectively). EE and DP were also associated with work hours (β = 0.02, p < 0.001; and β = 0.01, p < 0.001, respectively).

Conclusion

Nearly one-third of endocrinologists in China experienced burnout. Although there were no significant gender differences in the prevalence of overall burnout or EE, DP, and PA scores, male and female participants differed in factors associated with EE, DP, and PA. Interventions need to be tailored to target different aspects in male and female endocrinologists and target different subgroups.
Mother’s Parenting Stress and Marital Satisfaction During the Parenting Period: Examining the Role of Depression, Solitude, and Time Alone
Authors: Dong Simeng , Dong Qinnan , Chen Haiyan , Yang Shuai
Published: March 2022

This study examines the mechanism of maternal parenting stress on marital satisfaction based on the Vulnerability-Stress-Adaptation Model (VSAM), and draws on the needs theory to explore the role of alone time in marital relationships under different solitude preferences. The marital satisfaction Scale, Self-rating Depression Scale (SDS), Parenting Stress Scale (PSS), Preference for Solitude Scale (PSS), and alone time scale were used to conduct a questionnaire survey of 1,387 Chinese mothers in their parenting stage. The results found that: (1) in the overall group and the high and low solitude preference level group, depression plays a significant mediating role between parenting stress and marital satisfaction. (2) For mothers who prefer solitude, alone time can reduce the positive impact of parenting stress on depression, and but it cannot alleviate the negative impact of parenting stress and depression on marital satisfaction. (3) In the low solitude preference level group, alone time can aggravate the positive impact of parenting stress on depression and the negative impact of parenting stress on marital satisfaction.
The Utility of Research Domain Criteria in Diagnosis and Management of Dual Disorders: A Mini-Review
Authors: Hakak-Zargar Benyamin , Tamrakar Aarya , Voth Tessa , Sheikhi Armita , Multani Jennifer , Schütz Christian G.
Published: March 2022

The Research Domain Criteria (RDoC) initiative has been considered a comprehensive alternative classification framework for understanding neuropsychiatric ailments, as opposed to the longstanding, traditional DSM framework. Where the DSM categorizes neuropsychiatric disorders as each being distinct and diagnostically defined by the presence of specified symptoms, RDoC provides a multidimensional conceptualization of psychiatric disorders with neurobiological roots. By taking a multidimensional approach, RDoC overcomes two major constraints of the DSM framework: that is, that the DSM is categorical in its approach to psychiatric disorders to the point of understating the intersectionality between concomitant disorders, and that the DSM focuses mainly on clinical features. RDoC seems to better account for the intersection between dual disorders and considers a range of factors, from the more microscopic (e.g., genetics or molecular functions) to the more macroscopic (e.g., environmental influences). The multidimensional approach of RDoC is particularly appealing in the context of dual disorders. Dual disorders refers to a concurrent psychiatric disorder with an addiction disorder. RDoC accounts for the fact that there is often overlap in symptoms across and bidirectional influence between various disorders. However, to date, there is limited research into the clinical utility of RDoC, and less so in the context of the clinical management of dual disorders. In this Mini-Review, we discuss how RDoC differs from the DSM, what outcomes have been reported in utilizing RDoC clinically, the utility of RDoC for the diagnosis, management, and monitoring of psychopathology, and the limitations of RDoC as well as avenues for future research.
Evaluation of Pilot Manipulation Comfort in Different Flight Scenarios under Different Ambient Temperatures
Authors: Yang Xiufang , Sun Youchao , Wu Zhonglin , Wang Zongpeng
Published: March 2022

This study aimed to evaluate pilot manipulation comfort in different flight scenarios under different ambient temperatures. To achieve this goal, we designed a test plan to devise the physiological indexes used in the evaluation of pilot manipulation comfort, collected and analyzed pilot EMG signals and mean skin temperature, and on this basis revealed the impacts of different ambient temperatures and different flight missions on muscle activation and mean skin temperature. Based on this, we extracted muscle activation and mean skin temperature as the evaluation indices for the evaluation of manipulation comfort and established a model for this evaluation based on an improved particle swarm optimization and least-squares support vector machine (IPSO-LSSVM). Based on this model, the predicted manipulation comfort values of the participants were 4, 5 and 6, which belong to the categories of fair, somewhat comfortable and rather comfortable, respectively. By comparing the prediction results of different evaluation methods, the method proposed in this study was verified to be effective.
Design and Fabrication of a Phase Change Material Heat Storage Device for the Thermal Control of Electronics Components of Space Applications
Authors: Garmendia Iñaki , Vallejo Haritz , Seco Miguel , Anglada Eva
Published: March 2022

In this paper, the design and validation of a heat storage device based on phase change materials are presented, with the focus on improving the thermal control of micro-satellites. The main objective of the development is to provide a system that is able to keep electronics within safe temperature ranges during the operation of manoeuvres, while reducing mass and volume in comparison to other thermal control techniques. Due to the low thermal conductivity of phase change materials, the conductivity of the device as a whole is one of the major challenges of the development. This issue has been solved by means of the use of a lattice of aluminium fins. The thermal behaviour of the proposed solution is assessed with numerical simulation tools, and the results prove that the developed phase change material-based thermal control technique is able to provide the suitable integrated thermal management of micro-satellites. Fabrication challenges found in the project are also explained. Numerical results are validated through a testing stage. The predicted temperature profiles are in good agreement with experimental data and inside the range foreseen for the heat storage device.
Future Projection for Climate Suitability of Summer Maize in the North China Plain
Authors: Zhao Yanxi , Xiao Dengpan , Bai Huizi , Tang Jianzhao , Liu Deli
Published: March 2022

Climate change has and will continue to exert significant effects on social economy, natural environment, and human life. Research on the climatic suitability of crops is critical for mitigating and adapting to the negative impacts of climate change on crop production. In the study, we developed the climate suitability model of maize and investigated the climate suitability of summer maize during the base period (1981–2010) and two future periods of 2031–2060 (2040s) and 2071–2100 (2080s) in the North China Plain (NCP) based on BCC-CSM2-MR model (BCC) from the Coupled Model Comparison Program (CMIP6) under two Shared Socioeconomic Pathways (SSP) 245 and SSP585. The phenological shift of maize under future climate scenarios was simulated by the Agricultural Production Systems Simulator (APSIM). The results showed that the root mean square errors (RMSE) between observations and projections for sunshine suitability (SS), temperature suitability (ST), precipitation suitability (SP), and integrated climate suitability (SZ) during the whole growth period were 0.069, 0.072, 0.057, and 0.040, respectively. Overall, the BCC projections for climate suitability were in suitable consistency with the observations in the NCP. During 1981–2010, the SP, ST, and SZ were high in the north of the NCP and low in the south. The SP, ST, and SZ showed a downward trend under all the future climate scenarios in most areas of NCP while the SS increased. Therein, the change range of SP and SS was 0–0.1 under all the future climate scenarios. The ST declined by 0.1–0.2 in the future except for the decrease of more than 0.3 under the SSP585 scenario in the 2080s. The decrease in SZ in the 2040s and 2080s under both SSP scenarios varied from 0 to 0.2. Moreover, the optimum area decreases greatly under future scenarios while the suitable area increases significantly. Adjusting sowing data (SD) would have essential impacts on climate suitability. To some extent, delaying SD was beneficial to improve the climate suitability of summer maize in the NCP, especially under the SSP585 scenario in the 2080s. Our findings can not only provide data support for summer maize production to adapt to climate change but also help to propose agricultural management measures to cope with future climate change.
Interactions between Diffuse Light and Cucumber (Cucumis sativus L.) Canopy Structure, Simulations of Light Interception in Virtual Canopies
Authors: Zhang Yingyu , Yang Juan , van Haaften Marinus , Li Linyi , Lu Shenglian , Wen Weiliang , Zheng Xiuguo , Pan Jian , Qian Tingting
Published: March 2022

Plant photosynthesis and biomass production are associated with the amount of intercepted light, especially the light distribution inside the canopy. Three virtual canopies (n = 80, 3.25 plants/m2) were constructed based on average leaf size of the digitized plant structures: ‘small leaf’ (98.1 cm2), ‘medium leaf’ (163.0 cm2) and ‘big leaf’ (241.6 cm2). The ratios of diffuse light were set in three gradients (27.8%, 48.7%, 89.6%). The simulations of light interception were conducted under different ratios of diffuse light, before and after the normalization of incident radiation. With 226.1% more diffuse light, the result of light interception could increase by 34.4%. However, the 56.8% of reduced radiation caused by the increased proportion of diffuse light inhibited the advantage of diffuse light in terms of a 26.8% reduction in light interception. The big-leaf canopy had more mutual shading effects, but its larger leaf area intercepted 56.2% more light than the small-leaf canopy under the same light conditions. The small-leaf canopy showed higher efficiency in light penetration and higher light interception per unit of leaf area. The study implied the 3D structural model, an effective tool for quantitative analysis of the interaction between light and plant canopy structure.