bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and fabrication of a bioreactor for the proliferation of mesenchymal stem cells
Authors: De los Santos Morelia , Castruita Avila Laura , Zuñiga Aguilar Esmeralda , Ramirez Fernandez Odin
Published: December 2023

Currently, the number of organ donors is low and there are different methods to deal with this situation, including cell cultures, decellularization and bioreactors, which play an important role in helping the required processes to be carried out in a faster and more optimized way. The objective of this work is to make the cell proliferation process efficient using a bioreactor as an alternative, in which flow simulations can be made to previously analyze how it works. The radial flow hydraulic circuit was designed and simulated in SOLIDWORKS solving Navier-Stokes equations to verify that we have a non-turbulent laminar flow and simulate cell detachment without damaging the cell matrices. The design was printed with additive printing techniques, using photosensitive resin. Bioreactors also play a part in tissue engineering, as they allow for changes that can be monitored and reproduced in particular environmental factors. This article shows the successful design of a bioreactor to proliferate mesenchymal stem cells printed and subjected to simulations, whose results lead to the conclusion that there is non-turbulent laminar flow in the behavior of the system without subjecting the cells to a possible stress that causes damage.
INTEGRATION OF HEATING SYSTEM OPERATION
Authors: Selikhov Yu.A. , Rishchenko I.M. , Gorbunov K.A.
Published: December 2023

Ukraine has a huge asset in the form of existing district heating (DH) networks and demand for related services. DH is transforming the European energy sector, attracting efficient, local and renewable energy resources where this was not possible. However, due to the old material base of spare parts, insufficient level of investment and low level of management, Ukrainian DH systems are ineffective and do not meet the needs of consumers. The experience of European Union member states in central Europe shows that deep and sustained reforms can address the current challenges of the heating sector, including improving energy efficiency and economic competitiveness, strengthening environmental sustainability and enhancing energy security. The DH sector in Ukraine has not yet undergone the structural reform that other sectors of the economy have already gone through: over the past 30 years, the quality of services has decreased; subsidies have increased; the old material base is falling apart; The question arises whether it is worth continuing to support DH. As a result, Ukraine's DH sector is in dire straits, with financial, operational and technical problems compounding each other. Comprehensive and well-planned efforts must be made to create the conditions for exiting this state. Such structural reforms are essential to attracting the investment needed to improve the quality, efficiency and long-term potential of the district heating sector.

The article examines the possibility of using modern technological solutions to modernize heat supply systems in order to improve their efficiency, which can lead to improved district heating services, transparency and financial stability.

Keywords: energy resources, renewable energy sources, fossil fuels, automated heat and power plant, electricity, hot water, hot air, heating, private households, electric water heater, geothermal heat pump, wind power generator, low-power boiler house.
STUDY OF NON-FUEL APPLICATION OF BROWN COAL DERIVATIVES FOR BITUME MODIFICATION
Authors: Lebedev V. V. , Miroshnichenko D. V. , Lavrova I. O. , Cherkashina A. N.
Published: December 2023

The article shows research into the non-fuel use of brown coal derivatives for the modification of bitumen. The work used petroleum road grade bitumen BND 60/90 with a flash point in an open crucible of 260 °C, a softening temperature (using the ring and ball method) of 48 °C, resin after lignite thermal destruction and rubber waste. and powder. Based on the fact that the most relevant technologies for the non-fuel use of brown coal concern areas aimed at obtaining wax, humane preparations, adsorbents, obtaining valuable derivatives in the form of resins, GR, etc., an assessment was made of the non-fuel use of brown coal derivatives for modification bitumen. Analysis of the experimental results showed that the studied quality indicators of the composition samples, which include waste rubber powder, have an increased complex of both thermophysical and physical-mechanical characteristics. This is obviously due to the fact that, as a result of thermal destruction of rubber powder, the swelling process occurs faster compared to crumb rubber with a size of 2.5–4.5 mm. However, the process of destruction and dispersion in both cases does not occur completely, of course, and the volume of edematous rubber particles contains resins and polyaromatic components that affect the value of both thermophysical and physical-mechanical characteristics. Thus, it has been established that the optimal composition for creating effective polymer-modified bitumens with an increased complex of thermo-physical and physical-mechanical characteristics is 40 % by weight of rubber powder and 5 % by weight. brown coal resins after thermal destruction. It is shown that the results of laboratory studies have proven the prospects of using brown coal tar after thermal destruction for the modification of road bitumen. Summarizing research into the direction of non-fuel use of brown coal derivatives in the form of liquid products – tar resins of brown coal after thermal destruction for the modification of bitumen materials, it should be noted that the results obtained are moderate in comparison with existing directions for the production of polymer-modified bitumens. Modification of bitumen with primary and secondary polymers makes it possible to significantly improve their adhesion and performance characteristics compared to brown coal resins after thermal destruction. The use of brown coal resins after thermal destruction is much less effective in improving the elasticity, heat resistance and reducing the fragility of bitumen compositions compared to the use of thermoplastic and thermoelastomer modifiers in the production of polymer-modified bitumens.
HR MANAGEMENT AS A FACTOR OF INCREASING THE EFFICIENCY OF ENTERPRISES
Authors: Pohorielova Tetiana
Published: December 2023

The purpose of this article is to consider the problems associated with the impact of the effectiveness of personnel management on the efficiency of economic activity of enterprises. It is proved that the main source of economic development of the enterprise is its staff. The personnel of the enterprise is an important resource of the enterprise, the efficiency of which requires special attention. The role of human resources in highly efficient production is determined by their abilities, level of knowledge and qualifications, which are increasingly demanding today. Thus, one of the most effective ways to increase the economic development of an enterprise is to improve the efficiency of personnel management. It is noted that personnel management is a strategic function, the importance of which has grown with the inevitable need for globalization. The rapid growth of business globalization has created a fiercely competitive environment where the only effective way to stay competitive is to develop and improve the workforce. To achieve the goals set, the main areas of work of personnel managers are considered. Particular attention is paid to four main points that HR managers must analyze in order to create a healthy and effective strategic HR function. It is noted that the personnel management service must manage and develop it along with the overall strategy to establish the best match between the enterprise, its employees and the environment. This requires a systematic alignment of organizational goals and processes with the needs and capabilities of employees. This will allow employees to receive a variety of training and development opportunities. This is critical because businesses are more likely to gain a competitive advantage and there is a greater likelihood that overall organizational performance and communication between workers and the business will improve. It is concluded that the success of the enterprise, the degree of disclosure of its economic potential depend on the efficiency and rationality of management activities.
PROFIT FORMATION PROCESS AT THE ENTERPRISE AND EVALUATION OF ITS MANAGEMENT
Authors: Nazarova Tetjana , Maryna Shevchenko , Rybalchenko Inna
Published: December 2023

The article reveals the economic essence of profit, which is the result of the enterprise activity and the indicator that determines its efficiency, generalizes the interpretation of the authors and their approaches to the definition of "profit", identifies its common features in the works of scientists. It is proved that the need to understand the peculiarities of profit formation at the enterprise and its evaluation is due to the adoption of competent management decisions to maximize it. The mechanism of profit formation at the enterprise and its peculiarities are considered, the main factors of influence on the formation of enterprise profit are highlighted. The main sources of profit formation are systematized and the main goal of profit management at the enterprise is substantiated. It is determined that the main activity of the enterprise is the operating activity, so the assessment of the management of the process of profitability formation of the enterprise should be aimed at managing the process of profit formation from operating activities. It is noted that profit management at the enterprise is the process of developing and making effective management decisions in order to maximize the welfare of the owners of the enterprise in the current and future periods. The main groups of indicators for assessing the management of profit formation are allocated and the formulas for their calculation are indicated, which include: efficiency of use of fixed production assets, efficiency of labor use, efficiency of use of material resources, efficiency of use of working capital. It is proved that the assessment of management of the process of profitability formation of an economic entity also involves the assessment of profitability indicators.
INNOVATIVE DIRECTIONS OF ECONOMIC DEVELOPMENT OF THE GLOBAL LABOR MARKET IN GLOBALIZATION
Authors: Peredrii Andrea Elizabeth
Published: December 2023

The article analyzes the economic essence of the world labor market in the conditions of globalization. The world labor market is a complex economic system that is modified under the influence of the dynamics of demand and supply of labor resources in the world market; under the influence of the peculiarities of the formation of the price of labor, the formation of conditions and the amount of remuneration, the specifics of social protection; quantitative and qualitative movement and placement of labor in different countries of the world; differences in national approaches to the reproduction of the workforce, its professional training and retraining. In the conditions of globalization, national labor markets are quickly losing their closedness and isolation, becoming components of the world labor market. The strategic priorities of the development of the world labor market are formed under the influence of the multidirectional action of a number of factors: globalization and the openness of the national economy, structural and innovative shifts in the world economy, as a result of the intensification of labor migration and the strengthening of the role of international organizations as coordinators of the development of international economic relations. The main benefits of labor migration go to recipient countries. It is not easy to estimate the scale, because not all of them are quantifiable. They consist of savings on the training of specialists in host countries, the cost of products and services created by immigrants in the host country, and income from taxation. Immigrants, especially illegal immigrants, contribute more to the country's income than they receive from the public sector. International labor migration is becoming more and more global in nature. Today it covers the absolute majority of countries in the world. The main directions of modern migration processes are the North and South American region, the Western European market, Southeast and Western Asia, and the African section of migration movements. That is why these processes require regulation by the states participating in the exchange labor resources, and balanced international migration policy.
Primary morbidity of adolescents in the Vilyui group of districts of the republic of Sakha (Yakutia) in 1996–2021
Authors: Timofeev L. F. , Timofeev A. L.
Published: December 2023

The analysis of the primary morbidity of the adolescent population of the districts/uluses included in the Vilyui group (Verkhnevilyuisky, Vilyuisky, Mirninsky, Nyurbinsky and Suntarsky) has been carried out. First, we analyzed the morbidity data in the indicated uluses in a comparative aspect with all the administrative-territorial formations of the republic. At the same time, the levels of morbidity for certain classes of diseases were revealed in dynamics for the periods under consideration. It was found that, in terms of the incidence of adolescents, in general, the Suntarsky, Nyurbinsky and Verkhnevilyuisky uluses had a relatively better position, and the Vilyui ulus had a relatively worse position. In the Vilyui ulus, relatively high levels of primary morbidity are noted in 12 classes of diseases out of 17, while in the Nyurbinsky ulus – one each.
Intraoperative Ultrasound for Extracting Deep-Seated Radio-Opaque Foreign Body from Calf
Authors: Metikala Sreenivasulu , Munagala Venkata Manvith , Carroll Colin J. , Cheppalli Naga S.
Published: December 2023

Case:

A 50-year-old man presented with chronic refractory symptoms of radiating leg pain with muscle cramps because of a retained bullet in the calf after being shot in 1990. Radiographs confirmed the bullet lodged in posterolateral aspect of calf abutting proximal fibula. An intraoperative point-of-care ultrasound aided in accurate localization of bullet, thereby facilitating precise planning of surgical incision and subsequent removal.

Conclusions:

Ultrasound can be used as an alternative tool for safe surgical extraction of deep-seated metallic object with minimal tissue dissection, obviating the need for C-arm.
Shariah Framework Based on Qualities of Shariah Scholars Towards Islamic Fatwa Shopping in Nigeria
Authors: Yakasai Umar Musa , Muhamad Tijjani
Published: December 2023

This study examines the characteristics of Islamic scholars in issuing fatwas, highlighting concerns that financial institutions may seek fatwas aligning with their profit-driven motives, potentially contradicting Islamic principles. Focusing on Nigeria, it assesses the qualifications of advisors and supervisors in Islamic financial institutions and examines their link to the issuance of fatwas. Gathering insights from 250 randomly selected Shariah scholars and students, the research employs structural equation modeling (SEM) for analysis. The reliability of survey responses was assessed using a 5-point Likert scale. The consistency of the data was verified using mean-variance extracted, composite reliability, and Cronbach's alpha. SEM was used to assess the relationship between scholars' qualifications and the issuance of fatwas. The results underscore the importance of attributes such as knowledge, piety, sincerity, honesty, and legal responsibility in the issuance of fatwas, reflecting broader concerns about fatwa shopping. The study's scope was limited to Nigeria, focusing on scholars' perceptions of fatwa shopping
Sex and gender in perioperative cardiovascular research: a protocol for a scoping review
Authors: Sjaus Ana , Fakhory Nicole
Published: December 2023

Background
Inadequate inclusion of sex and gender in medical research has resulted in biased clinical guidance and disparities in knowledge and outcomes. Despite decades of efforts by regulatory and funding agencies, opportunities to generate sex specific knowledge are routinely neglected. Certain disciplines in cardiovascular medicine have made great strides, however, these advances have yet to permeate the literature on perioperative cardiovascular complications in non-cardiac surgery.
Prompted by the recent findings on sex-specific perioperative cardiovascular outcomes, this review aims to scope the literature in this field and categorize methodological approaches used to incorporate sex and gender as variables in this patient population.
Methods
Joanna Briggs Institute (JBI) methodology for scoping reviews will be followed in stages elaborated by Levac (2010). Detailed search strategy will be used to identify relevant primary research published since 2010. Screening against inclusion and exclusion criteria will be performed by independent reviewers. Data will be extracted from full text and additional materials of articles in the final selection. Results will be presented as proportions of studies reporting sex and gender, the assigned purpose of these variables in analysis and where they were reported in the article. In addition, articles will be mapped to the source, country of origin, and year of publication. Narrative summaries will be used to outline major findings and the depth of literature within each of the major topics (risk assessment/prediction, diagnosis, treatment, prognosis, and outcomes).
Discussion
Rising awareness of profound and complex implications of sex and gender in medicine has prompted calls for greater attention to participation equity, sex specific analysis and reporting. Focusing on perioperative cardiovascular complications, this study has the potential to identify knowledge gaps for further research, as well as areas of strength that could support formal knowledge synthesis or secondary analysis of data from past research.
Scoping Review Registration
Submitted on August 15th, 2023 (Web of Science osf.io/u25sf, pending approval)
The long-term impact of carbon emission trading and renewable energy support policy on China's power sector under the context of electricity marketed reform: an analysis based on system dynamics
Authors: Yue Wang , Haocheng Wei , Hao Shuai , Chen Xie , Xiaoyi Wang
Published: December 2023

China's power industry is currently undergoing a significant transition driven by the imperative of dual-carbon goal. To facilitate the successful transition of this sector, the government has implemented a series of targeted policies and reforms. These measures primarily encompass carbon emission trading (CET), renewable energy support policy, and electricity marketed reforms. The profound implications of these policies for China's power industry warrant investigation. To address this, this study constructs a comprehensive system dynamics model that delineates the intricate interplay between CET, renewable energy support policy, and electricity market reforms. Simulation results show that: (1) Under marketed on-grid price, electricity price exhibit a transient decline followed by a gradual ascent, peaking around 2045. This price trajectory stems from augmented power generation costs prompted by the imperative of carbon emission reduction and power sector transition. This finding diverges from some earlier studies suggesting a steep decline in electricity price following the adoption of marketed on-grid price. (2) CET can promote carbon reduction in power sector. Under the aegis of CET, carbon peaking within the sector is slated for realization by 2030. However, the introduction of renewable energy support policy yields some adverse effects on CET through price pass-through mechanisms. Yet, in parallel, it facilitates a notable enhancement in the overall proportion of renewable energy in end-use energy consumption. (3) A comparative evaluation of feed-in tariffs (FIT) and renewable portfolio standards (RPS) unveils distinct suitability profiles. FIT proves optimally aligned with the initial stages of renewable energy's pre-development and early expansion. In contrast, RPS manifests as an optimal choice post-technological maturation, concurrently alleviating the governmental financial load. (4) The context of marketed on-grid price augments the cost-effectiveness of carbon capture and storage (CCS). This incentivizes thermal power plants to embrace low-carbon technologies, fostering a conducive milieu for curtailing carbon emission. Based on these results, a number of policy implications are provided for the long-term development of China's power sector.
A mpox quadrivalent mRNA vaccine protects mice from a lethal vaccinia virus challenge
Authors: Chiu Sandra , Li Entao , Gong Qizan , Zhang Jiachen , Guo Xiaoping , Xie Wenyu , Chen Da , Shen Yanqiong , Hong Dongxiang , Li Zhihao , Wang Qianqian , Wang Chao , Wang Yucai
Published: December 2023

The outbreak of 2022 mpox virus (MPXV) infection in nonendemic regions is a global public health concern. A highly effective and safe MPXV vaccine that is available to the general public is urgently needed to control the MPXV pandemic. Here, we developed a polyvalent mRNA vaccine candidate, MPXV-1103, which expresses full-length B6R, A35R, A29L and M1R antigens with flexible linker-(G4S1)3 in a single sequence. Compared to the monovalent mpox mRNA vaccine candidates, MPXV-1103 elicits a stronger robust humoral response and MPXV-specific T-cell response and protects mice from a lethal vaccinia virus challenge with no live virus detected in the nasal and lungs even at dosages as low as 1 µg. Furthermore, the analysis of complete blood counts and photomicrographs of tissue in the main organs from mice vaccinated with MPXV-1103 at doses of 5 µg and 20 µg showed that two doses of MPXV-1103 vaccination did not cause observable pathologic changes in mice. Collectively, our results suggest that MPXV-1103, with features of high efficacy, safety and a simplified manufacturing process, is a promising vaccine candidate to defend against MPXV infection.
Autonomous Self-Healing Supramolecular Polymer Transistors for Skin Electronics
Authors: Oh Jin Young , Vo Ngoc Thanh Phuong , Jeong Min Woo , Kim Jun Su , Jung Kyu Ho , Lee Yeongjun , Ma Guorong , Gu Xiaodan , Tok Jeffrey , Nam Tae Uk , Lee Tae Il , Bao Zhenan
Published: December 2023

Skin-like field-effect transistors are key elements of bio-integrated devices for future user-interactive electronic-skin applications. Despite recent rapid developments in skin-like stretchable transistors, imparting self-healing ability while maintaining necessary electrical performance to these transistors remains a challenge. Herein, we describe a stretchable polymer transistor capable of autonomous self-healing. The active material consists of a blend of an electrically insulating supramolecular polymer with either semiconducting polymers or vapor-deposited metal nanoclusters. A key feature is to employ the same supramolecular self-healing polymer matrix for all active layers, i.e., conductor/semiconductor/dielectric layers, in the skin-like transistor. This provides adhesion and intimate contact between layers, which facilitates effective charge injection and transport under strain after self-healing. Finally, we fabricate skin-like self-healing circuits, including NAND and NOR gates and inverters, both of which are critical components of arithmetic logic units. This work greatly advances practical self-healing skin electronics.
Activation of IP10/CXCR3 signaling is highly coincidental with the deposition of PrPSc in the brains of scrapie-infected mice
Authors: Chen Jia , Chen Cao , Hu Chao , Yang Wei , Wang Lin , Chen Dong-Dong , Wu Yue-Zhang , Shi Qi , Dong Xiao-Ping
Published: December 2023

The chemokine IP10 and its receptor CXCR3 are activated in CNS during various neurodegenerative diseases. Our previous study demonstrated elevated levels of IP10 in the brains of several scrapie rodent models. However, the precise modulation of IP10/CXCR3 signaling in CNS during prion infection remains unresolved. Here, we observed an upregulation of IP10 signals primarily localized in neurons within the brains of scrapie-infected mice using various methodologies. Both CXCR3 levels and activation were significantly increased in the brains of scrapie-infected mice and in the prion-infected cell line SMB-S15. Enhanced CXCR3 expression was predominantly observed in neurons and activated microglia. Morphological colocalizations of PrPC/PrPSc with IP10 and CXCR3 were observed in the brains of scrapie-infected mice through IHC and immunofluorescence. IHC analysis using whole brain sections revealed increased accumulation of IP10 and CXCR3 specifically occurred in brain regions with higher levels of PrPSc deposits. Co-immunoprecipitation and biomolecular interaction assays suggested molecular interactions of PrP and IP10 as well as CXCR3. Notably, a significantly larger amount of IP10 accumulated within prion-infected SMB-S15 cells compared to the normal partner cell line SMB-PS. Importantly, treatment with resveratrol effectively suppressed prion replication in SMB-S15 cells, restoring the pattern of accumulation and secretion of cellular IP10 similar to that observed in SMB-PS cells. Our data presented herein demonstrate the activation of IP10/CXCR3 signaling in prion-infected brain tissues coinciding with the deposition of PrPSc. Modulating IP10/CXCR3 signaling in the brain represents a potential therapeutic target for mitigating the progression of prion diseases.
Physico- Chemical and Biological Investigations of Ocimum Basilicum L. Cultived in Algeria
Authors: Merzouki Soraya , Boukeria Sabah , Louafi Fadila , Alma Mohemet Hakki , Erenler Ramazan , Yeldiz Ilyas , Boulkroune Mina , Mouats Chabane
Published: December 2023

The study aims to explore biologically active substances from natural sources for potential medicines from the Ocimum basilicum L. plant. The MC-MS analysis reveals that the majority of compounds in the arial portion of this plant are Hesperidin and Vanillic acid, which are used in treating blood vessel fragility and neurological diseases like Alzheimer's and Parkinson's. The study conducted a qualitative phytochemical screening of the extract, revealing different secondary metabolites. Quantitative studies were conducted to determine the quantities of polyphenols, flavonoids, tannins, and alkaloids in the extract. Our investigations also assessed the extract's antioxidant activity using DPPH free radical scavenging and cyclic voltammetry, providing insights into its potential to combat oxidative stress. Finally, the hydrometanolic plant extract's anticoagulant activity was examined through Quick and Cephaline Kaolin time coagulation exploration tests, providing insights into its impact on coagulation pathways.
Assessment of the Pterygoid Hamulus Dimensions and Morphology in Iranian Women Using Cone-Beam Computed Tomography
Authors: Mahdavi Zahra , Hafezi Ladan
Published: December 2023

Objectives:
This study aimed to evaluate the morphology and dimensions of the pterygoid hamulus (PH) and its changes by ageing in 20-40-year-old women in the Dental Branch of the Islamic Azad University of Sciences, Tehran, Iran.
Materials and Methods:
This cross-sectional study was conducted on cone-beam computed tomography (CBCT) scans from 258 women aged 20-40. The morphology, length, width, and angle of the PH in coronal and sagittal planes were investigated.
Results:
The average length of the right and left hamulus was 5.50±1.37 and 5.37±1.36 mm. The average width of the right and left hamulus was 2.16±0.72 and 2.11±1.06 mm. The average coronal angle of the right and left hamulus was 22.3±9.79° and 30.16±8.99°. The average sagittal angle of the right and left hamulus was 26.11±7.26° and 26.34±7.63°. In contrast with the sagittal angle, the right and left hamulus’s length, width, and coronal angles were not symmetrical. Slender morphology was the most frequent morphology, and it was symmetrical only in the 20-30-year-old age group. Finally, no variable was affected by ageing.
Conclusions:
Hamulus dimensions and morphology did not change with ageing in women aged 20-40. The PH symmetry varied by age group. Also, CBCT was a suitable tool for investigating hamulus changes.
Clinical Relevance:
Understanding the characteristics of the PH is crucial for diagnosing and treating various oropharyngeal and craniofacial conditions. It also aids in interpreting radiographs and diagnosing unexplained oropharyngeal pains. CBCT is a valuable tool for precisely visualising these structures, offering clinical insights into this previously understudied area.
Identification of ZMIZ2 as a co-activator enhancing SIRT1 deacetylase activity in regulating Wnt and Hippo signalling pathways
Authors: Han Qiang , Gan Xueting , Feng Yuheng , Liu Yang , Lin Xuyong , Yu Xinmiao , Rong Xuezhu
Published: December 2023

Zinc finger MIZ-type containing 2 (ZMIZ2) is recognized as a ‘co-activator’ involved in embryonic development and the progression of certain malignant tumours. However, its expression and molecular mechanism in non-small cell lung cancer remain inadequately understood. Additionally, the relevant “assisted factors” of ZMIZ2 remain incompletely identified. Therefore, this study aims to investigate ZMIZ2 expression in non-small cell lung cancer tissue samples and cell lines through immunohistochemistry and immunoblotting. The goal is to establish its correlation with advanced pTNM staging, lymph node metastasis, and poor prognosis. Several in vivo and in vitro functional experiments have consistently shown that the introduction of ZMIZ2 promotes the proliferation, migration, and invasiveness of lung cancer cells, thus establishing its role as a promoter of oncogenes. Investigating the molecular mechanism, we applied mass spectrometry analysis and immunoprecipitation experiments, leading to the identification of the deacetylase Sirtuin 1 (SIRT1) as an ‘assisted key factor’ capable of interacting with ZMIZ2. Furthermore, KEGG enrichment analysis suggests that ZMIZ2 is closely related to the Wnt and Hippo pathways. Furthermore, we validated that the interaction between ZMIZ2 and SIRT1 enhances SIRT1 deacetylase activity. This direct downregulation of intranuclear β-catenin and YAP acetylation levels occurs independently of upstream proteins in the Wnt/Hippo pathways. It amplifies β-catenin-TCF4 and YAP-TEAD transcriptional activities, thereby regulating the Wnt and Hippo pathway. This results in the malignant transformation of lung cancer cells, providing a relevant experimental basis for searching for new biomarkers and the development of tumour-targeted medications.
Impact of Ce-Cu metal ions on Structural, Magneto-Electrical properties of Co2+ based nano ferrites and their application in Photo Catalytic activity
Authors: Yelagandula Shivakumar , Eragari Rajasheker Reddy , Gangadharaa R , Kotha Laxma Reddy , Adulla Panasa Reddy
Published: December 2023

Cobalt based nanoferrites Co1 − xCuxCeyFe2−yO4 Where\(x=0.0 to 0.5 with 0.1\) nano ferrites produced through citrate gel auto combustion technique and their structural and topographical studies were inspected through x-ray diffraction technique and field emission scanning electron microscope. The structural analysis confirms the prepared nano ferrites having single-phase cubic spinel, along Fd3m space group. Crystalline size of the synthesized samples found 20–26 nm calculated by Debey Scherrer formula. Catalytic activity of the samples confirmed by dye degradation studies of methylene blue and acid red dye degradation. Magnetic behavior of the materials measured by a vibrating sample magnetometer, saturation magnetization gradually decreases as increasing Cu and Ce content and found to be 26 to 18 emu/gm. Electrical properties were measured by LCR meter.
Quantitative performance assessment of Ultivue multiplex panels in formalin-fixed, paraffin-embedded human and murine tumor specimens
Authors: Ram Sripad , Mojtahedzadeh Sepideh , Aguilar Joan-Kristel , Coskran Timothy , Powell Eric , O'Neil Shawn
Published: December 2023

We present a rigorous validation strategy to evaluate the performance of Ultivue multiplex immunofluorescence panels. We have quantified the accuracy and precision of four different multiplex panels (three human and one mouse) in tumor specimens with varying levels of T cell density. Our results show that Ultivue panels are typically accurate wherein the relative difference in cell proportion between a multiplex image and a 1-plex image is less than 20% for a given biomarker. Ultivue panels exhibited relatively high intra-run precision (CV ≤ 20%) and relatively low inter-run precision (CV > > 20%) which can be remedied by using local intensity thresholding to gate biomarker positivity. We also evaluated the reproducibility of cell-cell distance estimates measured from multiplex images which show high intra- and inter-run precision. We introduce a new metric, multiplex labeling efficiency, which can be used to benchmark the overall fidelity of the multiplex data across multiple batch runs. Taken together our results provide a comprehensive characterization of Ultivue panels and offer practical guidelines for analyzing multiplex images.
Is the hyperdensity areas of the CT blend sign associated with the fresh bleeding in intracerebral hemorrhage?
Authors: Wu Qian , Che Wei , Chen Na , Wang Long , Ren Siying , Ye Fei , Zhao Xu , Wang Likun , Wu Guofeng
Published: December 2023

Background and purpose
Controversies still exist regarding the mechanism behind the formation of the CT blend sign in intracerebral hemorrhage (ICH). This study was undertaken to observe the relationship between the spot sign and the blend sign to gain insights into the blend sign's formation.
Methods
We collected patient data, including the spot sign's distribution, clinical information, and radiographic data. Patients were categorized based on the spot sign's location within the blend sign. We divided the cohort into two groups: (1) subjects with spot sign in the relatively hypodensity region of the blend sign(Hypo-spot sign group), (2) subjects with spot sign in the relatively hyperdensity region of blend sign(Hyper-spot sign group). Subsequently, based on hematoma expansion (HE), patients were grouped into either the hematoma expansion group (Expander group) or the no hematoma expansion group (No Expander group). After grouping, we analyzed the relationship between the spot sign and the blend sign, as well as the association between the blend sign and HE.
Results
A total of 205 patients were included in the study. Among them, 190 patients (92.7%) had the spot sign observed in the Hyper-spot sign group, while 55 patients (7.3%) had the spot sign identified in the Hypo-spot sign group. Significant hematoma growth was observed in 60 patients (29.3%) with HE, of which 59 patients (98.3%) had the spot sign detected in the Hyper-spot sign group, and only 1 patient (1.7%) had the spot sign found in the Hypo-spot sign group. Univariate logistic regression analysis revealed that Hyper-spot sign group (6.305, 1.810–49.072; p < 0.05) was an independent predictor of ICH growth. The sensitivity, specificity, positive predictive value, and negative predictive value of Hyper-spot sign group for predicting hematoma growth were 0.983, 0.096, 0.010, and 0.01, respectively.
Conclusions
In patients presenting both the blend sign and the spot sign, the spot sign was predominantly found in the denser region of the blend sign, indicating active bleeding in the denser part of the blend sign.
Trial registration: ClinicalTrials.gov, NCT05548530. Registered September 21, 2022,
Prognostic Analysis of Different Treatment Options for Cerebral Hemorrhage - Full Text View - ClinicalTrials.gov
Psychological and ethical issues raised by genomic in paediatric care pathway, a qualitative analysis with parents and childhood cancer patients
Authors: de Montgolfier Sandrine , Droin-Mollard Marion , Hervouet Lucile , Gimenez-Roqueplo Anne-Paule , Flahault Cécile , Petit Arnaud , Bourdeaut Franck , Julia Sophie , Rial-Sebbag Emmanuelle , Coupier Isabelle , Simaga Fatoumata , Brugieres Laurence , Guerrini-Rousseau Lea , Claret Beatrice , Cave Helene , Strullu Marion , Lahlou-Laforêt Khadija
Published: December 2023

In paediatric oncology, genomics raises new ethical, legal and psychological issues, as somatic and constitutional situations intersect throughout the care pathway. The discovery of potential predisposition in this context is sometimes carried out outside the usual framework. This article focuses on the views of children with cancer and their parents about their experience with genomic testing. 48 semi-structured interviews were performed with children with cancer and one of their parents, before and/or after receiving the genetic test results. The interviews were fully transcribed, coded and thematically analysed using an inductive method. This analysis revealed several themes that are key issues for parents and children: perceived understanding and consenting, apprehension about the test outcomes (expectations and fears), perception and attitude towards incidental findings. The main expectation for parents and children was an aetiological explanation. Children also emphasized the altruistic meaning of genetic testing, while parents seemed to expect a therapeutic and preventive approach for their child and the rest of the family. Parents were more concerned about a family risk, while children were more afraid of cancer relapse or transmission to their descendants. Both groups suggested possible feelings of guilt concerning family transmission and imaginary representations of what genomics may allow. Incidental findings were not understood by children, while some parents perceived the related issues and hesitated between wanting or not to know. A multidisciplinary step by step approach would be an interesting way to help parents and children to better grasp the complexity of genetic and/or genomic testing.
Association of exposure factors and their causal relationship with oral cancer: A Mendelian randomization study
Authors: Li Yunyao , Wang Kang , Li Xiaobing , Zhang Linqian
Published: December 2023

Objectives There is a strong association among risk factors for oral cancer (ORCA), such as smoking, alcohol consumption, fiber intake, and red meat intake. The apparent synergistic effects reported in previous observational studies may also underestimate the independent effects.Our study aims to further explore the potential etiology and causality of oral cancer.
Materials and Methods This study used the genome-wide association studies database (GWAS) for Mendelian randomization (MR) analysis to explore exposure factors associated with ORCA and detect the genetic causality between these exposures and ORCA risk.
Results Our results demonstrated that in univariate MR analysis, the five exposure factors (celery intake, average weekly beer and cider intake, spirits intake, and pork intake) were risk factors, and oily fish intake was a safety factor, but in multivariate MR analysis, pork intake had the greatest impact on oral cancer when the five food/drink intakes were simultaneously consumed.
Conclusions The causal relationship between the five exposure factors (oily fish intake, celery intake, pork intake, average weekly beer and cider intake, and spirits intake) and oral cancer was analyzed. The causal effects of pork on oral cancer may be underestimated.
Clinical Relevance Prevention of oral cancer requires better education about lifestyle-related risk factors, and improved awareness and tools for early diagnosis. Our study provides some risk factors that cannot be ignored for the cause prevention of oral cancer, such as pork intake, and its role in oral cancer prevention and control.
How to assess patient satisfaction in secondary unilateral cleft lip and palate rhinoplasty?
Authors: Fioravanti Alex Boso , Jorge Beatriz M. , Alonso Nivaldo
Published: December 2023

Background: This study aimed to compare two specific patient-reported outcomes (PROs) to evaluate the satisfaction and impact of secondary rhinoplasty on the quality of life of patients with cleft lip/palate (CL/P) and, determine if they are equivalent.
Design:This is a prospective study.
Setting: developed in a tertiary public hospital.
Patients: We selected patients who answered the ROE and CLEFT-Q questionnaires in pre and 12 months of postoperative surgery.
Methods: The questionnaire Rhinoplasty Outcomes Evaluation (ROE) allowed measure qualitative variables and CLEFT-Q was created for evaluation outcomes of appearance, facial function, and health-related quality of life (HR-QOL), however they are not equivalent.
Results: Eighteen patients participated in our study with an average old of 21.34±4.60 years. All patients have their scores improved in the ROE and CLEFT-Q questionnaires. The mean ROE pre-operatively score was 22.4±12.2 and postoperatively was 70.37±17.9 (p<0.05). The overall aesthetics concepts for CLEFT-Q pre-operatively score was 30.08±12.1 and the mean score post-operatively was 65.5±17.9 (p<0.05).
Conclusions: ROE and CLEFT-Q questionnaires are two useful tools for assessing the outcomes and quality of life after secondary rhinoseptoplasty surgery in CL/P. This surgery significantly improved patient quality of life regarding nose function and appearance. CLEFT-Q does not measure nasal airway function as ROE and for that reason, we suggest that some questions in this area be added to this one, making it complete. The scales used have similar accuracy and can be used to measure the satisfaction of patients with CL/P.
Trial Registration: This study was registered on the Brazilian clinical trials website (https://ensaiosclinicos.gov.br/rg/RBR-4wyfw3k), with the number U1111-1299-0385.
Extant and extinct bilby genomes combined with Indigenous knowledge improve conservation of a unique Australian marsupial
Authors: Hogg Carolyn , Edwards Richard , Farquharson Katherine , Silver Luke , Brandies Parice , Peel Emma , Escalona Merly , Jaya Frederick , Sun Rujiporn , Batley Kimberley , Bradford Tessa , Chang J King , Chen Zhiliang , Deshpande Nandan , Dziminski Martin , Ewart Kyle , Griffith Oliver , Marin-Gual Laia , Moon Katherine , Travouillon Kenny , Waters Paul , Whittington Camilla , Wilkins Marc , Helgen Kristofer , Lo Nathan , Ho Simon , Ruiz-Herrera Aurora , Paltridge Rachel , Graves Jennifer , Renfree Marilyn , Shapiro Beth , Ottewell Kym , Rangers Kiwirrkurra Community , Belov Katherine
Published: December 2023

The Ninu (Greater bilby, Macrotis lagotis) is a desert-dwelling, culturally and ecologically important marsupial. In collaboration with Indigenous rangers and conservation managers, we generated the first Ninu chromosome-level genome assembly (3.66 Gbp) and genome sequences for the extinct Yallara (lesser bilby, Macrotis leucura) to understand their biology and guide Ninu conservation management. Resequenced genomes (temperate Ninu=6; semi-arid Ninu=6; Yallara=4) revealed two major population crashes during global cooling events. Despite their 45-year long captive history, Ninu have fewer long runs of homozygosity than other larger mammals, which may be attributable to their boom-bust life-history. We assessed the beneficial impact of targeted conservation actions, like translocations, in the contemporary metapopulation (N=363 Ninu) and designed a SNP panel used by Indigenous rangers in monitoring remote wild populations. We dig into the unique Ninu biology using 12 tissue transcriptomes revealing expression of all 115 conserved eutherian chorioallantoic placentation genes in the uterus; an XY1Y2 sex chromosome system generated by fusion of the X with a large telocentric autosome; genes involved in anatomical and metabolic pathway adaptations to aridity; and expansions in olfactory receptor genes. Together, we demonstrate the holistic value of genomics in addressing key Indigenous and conservation management questions for ongoing conservation efforts.
Communities and Data Revolution
Authors: Vahabi Fataneh
Published: December 2023

The data revolution is transforming the way we interact with the world. With the rise of big data, organizations have access to an unprecedented amount of data, enabling them to better inform decision-making and develop new products and services. However, to capitalize on this data revolution, it is essential to build strong, connected communities to make full use of the data. By engaging stakeholders and developing a diverse and engaged community, organizations can better understand how to use data to gain insights, create new opportunities, and solve problems. This includes engaging with citizens, businesses, civil society organizations, and local government to ensure a wide range of perspectives are heard and incorporated into the data strategy. This kind of engagement also provides a platform for public dialogue and building trust in the data-driven decision-making process. Organizations should also invest in data literacy initiatives to ensure that data is used effectively. These initiatives should be tailored to the needs of the community and focus on educating stakeholders on the responsible use of data. This could include workshops, training sessions, and hackathons to familiarize the community with data analysis, data visualization, and data storytelling. Finally, organizations should develop data sharing protocols to ensure that data is shared securely and ethically. This includes having clear terms and conditions regarding data usage, data protection, and data security. It also involves developing policies to ensure that data is used responsibly and in the public interest.
Hydrogeological characterization and extended seasonal hydrogeochemical monitoring of a CO₂ controlled-release experimental site: the case of TECNOPUC-Viamão, Rio Grande do Sul state, Southern Brazil
Authors: Zielinski João Pedro Tauscheck , Hamerski Fernando , Vecchia Felipe Dalla , Melo Clarissa Lovato , Reginato Pedro Antônio Roehe
Published: December 2023

Reduced-scale CO2 release experiments in shallow aquifers serve as crucial monitoring strategies for detecting unintended CO2 leakage into potable aquifers within Carbon Capture and Storage (CCS) projects. Understanding site-specific geological, hydrogeological, and climatic features is essential. However, accurately tracing changes in groundwater quality due to this process and using hydrochemical parameters for CO2 leakage diagnosis require establishing a solid, seasonally relevant baseline to avoid misinterpretation. This study focuses on detailing the geological, hydrogeological, and geophysical characteristics of the TECNOPUC-Viamão CO2 controlled-release experimental site in Rio Grande do Sul, Brazil. It presents a comprehensive three-year investigation into the seasonal natural background hydrochemistry. Field characterization involved recognition boreholes, in-situ infiltration and Slug Tests, topographic surveys, DC resistivity measurements, and groundwater sampling campaigns for physicochemical, major, minor, trace elements, and δ¹³C-DIC evaluation. Results indicate the area comprises a granite-derived multilayer phreatic aquifer with two distinct hydrostratigraphic units (St and Aa). These units differ in lithological composition, hydraulic conductivities (St: 10− 4 m/s, Aa: 10− 8 m/s), apparent resistivities, and physicochemical and hydrochemical compositions. The St unit shows slightly neutral pH, higher temperature, EC, ORP, DO, Ca, Mg, K, Fe, Mn, Sr, B, HCO3−, and DIC concentrations, with δ¹³C-DIC between − 3 to -8‰. Conversely, the Aa unit displays slightly acidic pH, lower temperature, EC, ORP, higher DO fluctuation, Na, SO42−, Cl−, NO3−, Zn, Al, Ni concentrations, lower HCO3−, DIC levels, with δ¹³C-DIC ranging between − 6 to -11‰. Additionally, seasonal monitoring campaign revealed that there is a clear temperature-related influence on Ca, Mg, K, Na, NO₃⁻, and trace elements (Fe, Ba, Sr, Mn, Al, B, Ni, and Zn) behavior, which could impact further interpretation of the results during the upcoming CO2 injection phase, and that the Control Charts can confidently serve as a valuable tool in understanding the inherent natural hydrochemical trends.