bluuebunny/crossref_metadata_2025 huggingface dataset:
Utility of promoter hypermethylation in malignant risk stratification of intraductal papillary mucinous neoplasms
Authors: Chhoda Ankit , Sharma Anup , Sailo Bethsebie , Tang Haoyu , Ruzgar Nensi , Tan Wan Ying , Ying Lee , Khatri Rishabh , Narayanan Anand , Mane Shrikant , De Kumar Bony , Wood Laura D. , Iacobuzio-Donahue Christine , Wolfgang Christopher L. , Kunstman John W. , Salem Ronald R. , Farrell James J. , Ahuja Nita
Published: February 2023

Background

Intraductal papillary mucinous neoplasms (IPMNs), a type of cystic pancreatic cancer (PC) precursors, are increasingly identified on cross-sectional imaging and present a significant diagnostic challenge. While surgical resection of IPMN-related advanced neoplasia, i.e., IPMN-related high-grade dysplasia or PC, is an essential early PC detection strategy, resection is not recommended for IPMN-low-grade dysplasia (LGD) due to minimal risk of carcinogenesis, and significant procedural risks. Based on their promising results in prior validation studies targeting early detection of classical PC, DNA hypermethylation-based markers may serve as a biomarker for malignant risk stratification of IPMNs. This study investigates our DNA methylation-based PC biomarker panel (ADAMTS1, BNC1, and CACNA1G genes) in differentiating IPMN-advanced neoplasia from IPMN-LGDs.

Methods

Our previously described genome-wide pharmaco-epigenetic method identified multiple genes as potential targets for PC detection. The combination was further optimized and validated for early detection of classical PC in previous case–control studies. These promising genes were evaluated among micro-dissected IPMN tissue (IPMN-LGD: 35, IPMN-advanced neoplasia: 35) through Methylation-Specific PCR. The discriminant capacity of individual and combination of genes were delineated through Receiver Operating Characteristics curve analysis.

Results

As compared to IPMN-LGDs, IPMN-advanced neoplasia had higher hypermethylation frequency of candidate genes: ADAMTS1 (60% vs. 14%), BNC1 (66% vs. 3%), and CACGNA1G (25% vs. 0%). We observed Area Under Curve (AUC) values of 0.73 for ADAMTS1, 0.81 for BNC1, and 0.63 for CACNA1G genes. The combination of the BNC1/ CACNA1G genes resulted in an AUC of 0.84, sensitivity of 71%, and specificity of 97%. Combining the methylation status of the BNC1/CACNA1G genes, blood-based CA19-9, and IPMN lesion size enhanced the AUC to 0.92.

Conclusion

DNA-methylation based biomarkers have shown a high diagnostic specificity and moderate sensitivity for differentiating IPMN-advanced neoplasia from LGDs. Addition of specific methylation targets can improve the accuracy of the methylation biomarker panel and enable the development of noninvasive IPMN stratification biomarkers.
Prevalence and factors associated with adolescent pregnancies in Zambia: a systematic review from 2000–2022
Authors: Malunga Gift , Sangong Sidney , Saah Farrukh Ishaque , Bain Luchuo Engelbert
Published: February 2023

Background

Adolescent pregnancy increases risk of short- and long-term adverse social and health outcomes for the adolescent mother and child. Zambia has high prevalence rates of adolescent pregnancy. However, the risk factors are varied and in need of further review and research. The study accordingly reviewed the prevalence and factors associated with adolescent pregnancy in Zambia.

Methods

This systematic review was conducted following the 2020 Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The review included original peer-reviewed research articles published from 2000 onwards in English, retrieved from Medline, EMBASE, CINAHL, and African Journals Online databases. Thematic synthesis was used in the analysis of the data extracted from the included studies.

Results

Six research studies carried out in Zambia (two quantitative, two qualitative, and two mixed methods) were reviewed and included. Prevalence of adolescent pregnancy in Zambia ranged from 29 to 48%. Additionally, it was found that 29.1% of the country’s adolescents, nationally, had given birth as of 2018. Factors at an individual’s level such as early or child marriage, exposure to media, knowledge about sexual and reproductive health (SRH) and contraception, contraceptive use, as well as risky sexual behaviours were found to be significantly associated with adolescent pregnancy. Peer pressure, educational attainment, household wealth, and the power dynamics of the household head were identified as the major socio-economic factors alongside socio-cultural, gender and sexual norms amongst other environmental and contextual factors. Policy level factors identified were lack and limited access to SRH information and services by adolescents, including an enabling legal environment.

Conclusion

From the review, it was abundantly clear that a combination of individual, interpersonal, environmental, and an enabling legal/policy level factors significantly contribute to the high levels of adolescent pregnancy. There is a paucity of empirical research on the prevalence and determinants of adolescent pregnancy, which suggests an imperative need for large multi-site mixed methods studies to properly explore these and other determinants on a national scale, as well as the long-term implications of these pregnancies on adolescent mothers and babies. Multifaceted and multisectoral interventions which include improved access to education, economic empowerment, addressing gender and socio-cultural norms, should be implemented having due regard to the socio-cultural context which should ride on strong political will, failing which adolescent girls in Zambia will definitely be left behind.
Pro-fibrotic and apoptotic activities of circARAP1 in myocardial ischemia–reperfusion injury
Authors: Li Xi , Guo Lei , Wang Jingjing , Yang Xing
Published: February 2023

Reperfusion modality can cause damage to cardiomyocytes, known as myocardial ischemia–reperfusion injury (MI/RI). Circular RNAs (circRNAs) are fundamental regulators associated with many cardiac diseases, including MI/RI. However, their functional impact on cardiomyocyte fibrosis and apoptosis remains elusive. Therefore, this study aimed to explore possible molecular mechanisms of circARPA1 in animal models and in hypoxia/reoxygenation (H/R)-treated cardiomyocytes. GEO dataset analysis showed that has_circ_0023461 (circARPA1) was differentially expressed in myocardial infarction samples. Real-time quantitative PCR further supported that circARPA1 was expressed at high levels in animal models and in H/R-triggered cardiomyocytes. Then, loss-of-function assays were performed to show that circARAP1 suppression effectively ameliorated cardiomyocyte fibrosis and apoptosis in MI/RI mice. Mechanistic experiments showed that miR-379-5p, KLF9 and Wnt signaling pathways were associated with circARPA1. circARPA1 can sponge miR-379-5p to regulate KLF9 expression, thereby activating the wnt/β-catenin pathway. Finally, gain-of-function assays revealed that circARAP1 aggravated MI/RI in mice and H/R-induced cardiomyocyte injury by regulating the miR-379-5p/KLF9 axis to activate Wnt/β-catenin signaling.
Research trends and hotspots evolution of cardiac amyloidosis: a bibliometric analysis from 2000 to 2022
Authors: Fu Zhenyue , Lv Jiayu , Gao Xiya , Zhang Bingxuan , Li Yumeng , Xu Xia , Zheng Haoran , Wu Huaqin , Song Qingqiao
Published: February 2023

In the new century, cardiac amyloidosis has received more attention from many countries and institutions, leading to innovations in the essence of the pathology, biological markers, noninvasive tests, and staging diagnoses and treatments for this disease. However, few reviews have summarized the research trends and hotspots in cardiac amyloidosis. Bibliometrics analysis is a statistically based approach to research that visualizes the contributions of academic institutions and changes in research hotspots. Therefore, in this paper, we used Citespace and VOSviewer software to conduct co-occurrence analysis and collaborative network analysis on the countries, institutions, and authors in the articles related to cardiac amyloidosis since the new century. And further find out burst keywords and references to obtain the research history, disciplinary development, and new hotspots and topics.
The prognostic value of tumor mutation burden (TMB) and its relationship with immune infiltration in breast cancer patients
Authors: Cui Shengjin , Feng Jingying , Tang Xi , Lou Shuang , Guo Weiquan , Xiao Xiaowei , Li Shuping , Chen Xue , Huan Yu , Zhou Yiwen , Xiao Lijia
Published: February 2023

Objective

Although the tumor mutation burden (TMB) was reported as a biomarker for immunotherapy of various cancers, whether it can effectively predict the survival prognosis in breast cancer patients remains unclear. In this study, the prognostic value of TMB and its correlation with immune infiltration were explored by using multigroup studies.

Methods

The somatic mutation data of 986 breast cancer patients were obtained from TCGA database. Breast cancer patients were divided into a low-TMB group and a high-TMB group according to the quartile of TMB scores. The differentially expressed genes (DEGs) were identified by the “limma” R program. The CIBERSORT algorithm was utilized to estimate the immune cell fraction of each sample. The TIMER database was utilized to evaluate the association between CNVs of immune genes and tumor immune cell infiltration and the prognostic value of the immune cells in breast cancer.

Results

In breast cancer, TP53, PIK3CA, TTN, CDH1 and other genes were the most important mutated genes. Higher survival rate of patients was found in the low-TMB group. Among the top 10 DEGs, three of them belong to the KRT gene family. GSEA enrichment analysis showed that MAPK, Hedgehog, mTOR, TGF-bate and GnRH signaling pathways were enriched in the low-TMB group. The infiltration levels of the most of immune cells were higher in the low-TMB group (P < 0.01). Higher expression of CCL18 and TRGC1 was correlated with poor prognosis. Breast cancer patients with CCL18 copy number variations, especially arm-level gains, showed significantly decreased immune cell infiltration. In the low B cell infiltration group, the survival prognosis of breast cancer patients was poor.

Conclusions

TMB is a potential prognosis marker in breast cancer. Immune-related gene CCL18 and TRGC1 are biomarkers of poor prognosis while immune (B cell) infiltration is a biomarker of good prognosis.
Energy landscapes of Kodiak brown bears: a comparison of accelerometer and global positioning system-derived estimates
Authors: Finnegan S. P. , Pagano A. M. , Svoboda N. J. , Schooler S. L. , Belant J. L.
Published: February 2023

Within optimal foraging theory animals should maximize their net energy gain while minimizing energetic costs. Energetic expenditure in wild animals is therefore key to measure proxies of fitness. Accelerometers are an effective tool to study animal movement-based energetics, but retrieval of the device is usually required and often difficult. Accelerometers measure movement across three axes (x, y, and z) and can be calibrated to measures of oxygen consumption from captive animals, providing estimates of overall energy expenditure. Measuring energetic expenditures using a global positioning system (GPS) approach could provide an alternative method to study energetic ecology. This technique uses locomotor speeds across a range of slopes from successive GPS locations, which can be linked to the energy expenditure from captive individuals. We compared accelerometer and GPS methods of energetic expenditures in free-roaming brown bears (Ursus arctos) on the Kodiak Archipelago, Alaska, USA. We then applied the GPS method to examine how multiple factors influenced brown bear movement-based daily energetic expenditures (MDEE). We found that while the two energetic measurements differed (Wilcoxon signed rank test: V = 2116, p < 0.001), they were positively correlated (r = 0.82, p < 0.001). The GPS method on average provided 1.6 times greater energy estimates than the accelerometer method. Brown bears had lower MDEE during periods of high food abundance, supporting optimal foraging theory. Reproductive status and age did not influence MDEE, however movement rates had a positive linear relationship. Energetic ecology is important for understanding drivers of animal movements. Data from GPS collars can provide useful information on energetic expenditures, but should be validated for the specific taxa, ecosystem, and GPS sampling rate used. Additionally, while movement-based estimates of energy expenditure can elucidate the mechanisms driving habitat use decisions, they may not fully reflect an animal’s overall energy demands. Brown bear movement-based energetic expenditure was influenced by food abundance and movement rates, which highlighted the importance of access to prime foraging sites to enhance energetic efficiency.
Survival benefit and biomarker analysis of pyrotinib or pyrotinib plus capecitabine for patients with HER2-positive metastatic breast cancer: a pooled analysis of two phase I studies
Authors: Guan Xiuwen , Ma Fei , Li Qiao , Chen Shanshan , Lan Bo , Fan Ying , Wang Jiayu , Luo Yang , Cai Ruigang , Zhang Pin , Li Qing , Xu Binghe
Published: February 2023

Background

Pyrotinib, a novel irreversible tyrosine kinase inhibitor (TKI), has demonstrated promising antitumor activity to improve the overall response rate and progression-free survival (PFS) in patients with HER2-positive metastatic breast cancer (MBC). However, the survival data of pyrotinib or pyrotinib plus capecitabine in HER2-positive MBC remains scarce. Thus, we summarized the updated individual patient data from the phase I trials of pyrotinib or pyrotinib plus capecitabine, to provide a cumulative assessment on long-term outcomes and associated biomarker analysis of irreversible TKIs in HER2-positive MBC patients.

Methods

We performed a pooled analysis of the phase I trials for pyrotinib or pyrotinib plus capecitabine based on the updated survival data from individual patients. Next-generation sequencing was performed on circulating tumor DNA for predictive biomarkers.

Results

A total of 66 patients were enrolled, including 38 patients from the phase Ib trial for pyrotinib and 28 patients from the phase Ic trial for pyrotinib plus capecitabine. The median follow-up duration was 84.2 months (95% CI: 74.7–93.7 months). The estimated median PFS in the entire cohort was 9.2 months (95% CI: 5.4–12.9 months) and median OS was 31.0 months (95% CI: 16.5–45.5 months). The median PFS was 8.2 months in the pyrotinib monotherapy cohort and 22.1 months in the pyrotinib plus capecitabine group, while the median OS was 27.1 months in the pyrotinib monotherapy group and 37.4 months in the pyrotinib plus capecitabine group. Biomarker analysis suggested that the patients harbored concomitant mutations from multiple pathways in HER2-related signaling network (HER2 bypass signaling pathways, PI3K/Akt/mTOR pathway and TP53) were observed with significantly poorer PFS and OS when compared to those with none or one genetic alteration (median PFS, 7.3 vs. 26.1 months, P = 0.003; median OS, 25.1 vs. 48.0 months, P = 0.013).

Conclusions

The updated survival results based on individual patient data from the phase I trials of pyrotinib-based regimen revealed promising PFS and OS in HER2-positive MBC. Concomitant mutations from multiple pathways in HER2-related signaling network may be a potential efficacy and prognosis biomarker for pyrotinib in HER2-positive MBC.

Trial registration

ClinicalTrials.gov. (NCT01937689, NCT02361112).
Metagenomics and metabarcoding experimental choices and their impact on microbial community characterization in freshwater recirculating aquaculture systems
Authors: Rieder Jessica , Kapopoulou Adamandia , Bank Claudia , Adrian-Kalchhauser Irene
Published: February 2023

Background

Microbial communities in recirculating aquaculture systems (RAS) play a role in system success, nutrient cycling, and water quality. Considering the increasing socio-economic role of fish farming, e.g., regarding food security, an in-depth understanding of aquaculture microbial communities is also relevant from a management perspective, especially regarding the growth, development, and welfare of the farmed animal. However, the current data on the composition of microbial communities within RAS is patchy, which is partly attributable to diverging method choices that render comparative analyses challenging. Therefore, there is a need for accurate, standardized, and user-friendly methods to study microbial communities in aquaculture systems.

Results

We compared sequencing approach performances (3 types of 16S short amplicon sequencing, PacBio long-read amplicon sequencing, and amplification-free shotgun metagenomics) in the characterization of microbial communities in two commercial RAS fish farms. Results showed that 16S primer choice and amplicon length affect some values (e.g., diversity measures, number of assigned taxa or distinguishing ASVs) but have no impact on spatio-temporal patterns between sample types, farms and time points. This implies that 16S rRNA approaches are adequate for community studies. The long-read amplicons underperformed regarding the quantitative resolution of spatio-temporal patterns but were suited to identify functional services, e.g., nitrification cycling and the detection of pathogens. Finally, shotgun metagenomics extended the picture to fungi, viruses, and bacteriophages, opening avenues for exploring inter-domain interactions. All sequencing datasets agreed on major prokaryotic players, such as Actinobacteriota, Bacteroidota, Nitrospirota, and Proteobacteria.

Conclusion

The different sequencing approaches yielded overlapping and highly complementary results, with each contributing unique data not obtainable with the other approaches. We conclude that a tiered approach constitutes a strategy for obtaining the maximum amount of information on aquaculture microbial communities and can inform basic research on community evolution dynamics. For specific and/or applied questions, single-method approaches are more practical and cost-effective and could lead to better farm management practices.
Therapeutic plasma exchange in postpartum HELLP syndrome: a case report
Authors: Kojima Nana , Kuroda Kosuke , Tani Makiko , Kanazawa Tomoyuki , Shimizu Kazuyoshi , Maki Jota , Masuyama Hisashi , Morimatsu Hiroshi
Published: February 2023

Background

Postpartum hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome is more difficult to treat than HELLP syndrome during pregnancy. We describe a case of postpartum HELLP syndrome that responded to plasma exchange (PE) therapy.

Case presentation

A 30-year-old primipara woman was hospitalized for gestational hypertension at 33 weeks of gestation and underwent an emergent cesarean section at 36 weeks and 6 days of gestation due to rapidly progressing pulmonary edema. After delivery, liver dysfunction and a rapid decrease in platelet count were observed, and the patient was diagnosed with severe HELLP syndrome. She experienced multiple organ failure despite intensive care, and PE therapy was initiated. Her general condition dramatically stabilized within a few hours of PE therapy.

Conclusion

It is controversial whether PE therapy should be used primarily in the management of HELLP syndrome, but early initiation of PE therapy could be effective for severe HELLP syndrome.
The COVID-19 pandemic: knowledge, attitudes and practices of coronavirus (COVID-19) among patients with type 2 diabetes
Authors: Mohamadian Hashem , faraji Amrullah , Ghorrabi Ahmad Tahmasebi , Ghobadi-Dashdebi Kamel , Salahshouri Arash
Published: February 2023

Background

It is necessary to evaluate COVID-19 data on Knowledge, Attitudes and Practices (KAP) to confirm effective protective practice and to reduce risk in society. Hence, the study was carried out to determine KAP towards COVID-19 and the factors associated with knowledge and practices among patients with type 2 diabetes (T2D).

Methods

In this cross-sectional (descriptive-analytical) study, 357 patients with diabetes in Izeh participated in the study. The sampling method used was convenience sampling method. Data collection tool was a researcher-made questionnaire of demographic information and KAP of patients with T2D in relation to the new coronavirus. The validity and reliability of the research tool was confirmed using the content validity and test–retest. Data analysis was done in Stata.14.2 and Smartpls 3.2.8 using descriptive and analytical statistical tests.

Results

The mean score of participants' KAP towards Covid-19 was 74.22 (16.30), 72.88 (14.87), and 70.51 (19.70), respectively. The lowest and the highest score of the patients' knowledge was in the field of transmission (56.60 (20.96)) and care and prevention of the COVID-19 (88.58 (21.88)), respectively. Residence was the most important factor predicting the practice of diabetic patients with an explanatory coefficient ([SMD 1.08 (95% CI 0.85 to 1.30),P < 0.001] and R2 = 0.87%).

Conclusion

Despite the good level of KAP of people towards the COVID-19 disease, there were answers showing poor knowledge, incorrect beliefs and attitudes, and insufficient practice regarding different aspects of the COVID-19 in some cases in our study. Residence was a strong predictor of type 2 diabetes mellitus (T2DM) patients’ practice in terms of protective behaviors against Covid-19. Hence, educational needs evaluation based on residence is recommended, especially in rural T2DM patients.
Reframing data ethics in research methods education: a pathway to critical data literacy
Authors: Atenas Javiera , Havemann Leo , Timmermann Cristian
Published: February 2023

This paper presents an ethical framework designed to support the development of critical data literacy for research methods courses and data training programmes in higher education. The framework we present draws upon our reviews of literature, course syllabi and existing frameworks on data ethics. For this research we reviewed 250 research methods syllabi from across the disciplines, as well as 80 syllabi from data science programmes to understand how or if data ethics was taught. We also reviewed 12 data ethics frameworks drawn from different sectors. Finally, we reviewed an extensive and diverse body of literature about data practices, research ethics, data ethics and critical data literacy, in order to develop a transversal model that can be adopted across higher education. To promote and support ethical approaches to the collection and use of data, ethics training must go beyond securing informed consent to enable a critical understanding of the techno-centric environment and the intersecting hierarchies of power embedded in technology and data. By fostering ethics as a method, educators can enable research that protects vulnerable groups and empower communities.
Facilitators and barriers to implementing electronic patient-reported outcome and experience measures in a health care setting: a systematic review
Authors: Glenwright Ben G. , Simmich Joshua , Cottrell Michelle , O’Leary Shaun P. , Sullivan Clair , Pole Jason D. , Russell Trevor
Published: February 2023

Objective

This systematic literature review aimed to identify factors that influence the implementation of electronic patient-reported outcome measures (ePROMs) and patient-reported experience measures (ePREMs) in healthcare settings.

Introduction

Improvements in health care through increased patient engagement have gained traction in recent years. Patient-reported outcome measures (PROMs) and patient-reported experience measures (PREMs) are tools used to improve the quality of care from the patient perspective. The influence of implementing PROMs and PREMs using electronic information systems (ePROMs and ePREMs) is not well understood.

Inclusion criteria

Studies with information related to the implementation of ePROMs and/or ePREMs with a focus on health-related services, irrespective of provider type, were included.

Methods

A literature search of peer-reviewed databases was conducted on the 24th of January 2022 for articles about barriers and facilitators of the implementation of ePROMs/ePREMs in healthcare settings. Two reviewers independently extracted relevant findings from the included studies and performed a descriptive code-based synthesis before collaboratively creating a final consensus set of code categories, which were then mapped to the consolidated framework of implementation research (CFIR). Study quality was appraised using a mixed-methods appraisal tool (MMAT).

Results

24 studies were eligible for inclusion in the screening of 626 nonduplicate studies. Quality assessment using the MMAT revealed that 20/24 studies met at least 60% of the MMAT criteria. Ninety-six code categories were identified and mapped to the constructs across all CFIR domains.

Conclusion

To guide the effective implementation of ePROMs/ePREMs in healthcare settings, factors shown to influence their implementation have been summarised as an implementation checklist for adoption and use by clinicians, organisations, and policymakers.
Patient-reported outcome measures used to improve youth mental health services: a systematic review
Authors: Thapa Bajgain Kalpana , Amarbayan Mungunzul , Wittevrongel Krystle , McCabe Erin , Naqvi Syeda Farwa , Tang Karen , Aghajafari Fariba , Zwicker Jennifer D. , Santana Maria
Published: February 2023

Background

Patient-reported outcome measures (PROMs) are standardized and validated self-administered questionnaires that assess whether healthcare interventions and practices improve patients’ health and quality of life. PROMs are commonly implemented in children and youth mental health services, as they increasingly emphasize patient-centered care. The objective of this study was to identify and describe the PROMs that are currently in use with children and youth living with mental health conditions (MHCs).

Methods

Three databases (MEDLINE, EMBASE, and PsycINFO) were systematically searched that used PROMs with children and youth < 18 years of age living with at least one diagnosed MHC. All methods were noted according to Preferred Reporting Items for Systematic reviews and Meta-Analysis. Four independent reviewers extracted data, which included study characteristics (country, year), setting, the type of MHC under investigation, how the PROMs were used, type of respondent, number of items, domain descriptors, and the psychometric properties.

Results

Of the 5004 articles returned by the electronic search, 34 full-texts were included in this review. This review identified both generic and disease-specific PROMs, and of the 28 measures identified, 13 were generic, two were generic preference-based, and 13 were disease-specific.

Conclusion

This review shows there is a diverse array of PROMs used in children and youth living with MHCs. Integrating PROMs into the routine clinical care of youth living with MHCs could improve the mental health of youth. Further research on how relevant these PROMs are children and youth with mental health conditions will help establish more uniformity in the use of PROMs for this population.
Atypical presentation of polymorphous low-grade neuroepithelial tumor of young (PLNTY): a case report
Authors: Joshi Vijay , Chougule Meghana V. , Mudkanna Anand , Mishra Rakesh Kumar , Maurya Ved Prakash , Agrawal Amit
Published: February 2023

The polymorphous low-grade neuroepithelial tumor of young (PLNTY) is considered as one among the low-grade neuroepithelial tumor; as per WHO-2021 classification of Brain Tumors in the fifth edition. The term PLNTY was first coined by Huse in 2016. These morphologically variable tumors are characterized by their oligodendroglioma-like cellular components, infiltrative growth pattern, and Cluster of Differentiation 34 (CD34) immunopositivity. Frequent genetic abnormalities involving mitogen-activated protein kinase pathway constituents like the BRAF proto-oncogene or fibroblast growth receptor 2/3 are harbored by PLNTYs. Radiologically, these are found to be well-circumscribed lesions with calcified and cystic components, affecting primarily temporal lobes. Clinically, they present with seizures/epilepsy in young adults (< 30 years). In the present manuscript we are reporting a case of 37-year-old male, presenting with a gradually progressive headache for 6 months, found to have a left frontal multiloculated cystic lesion with dystrophic calcifications. Based on the distinctive histopathological feature of the oligodendroglioma-like infiltrative lesion showing CD34 immunopositivity, a diagnosis of a polymorphous low-grade neuroepithelial tumor of young was made.
Can resistance to either isoniazid or rifampicin predict multidrug resistance tuberculosis (MDR-TB)
Authors: Mchaki Betty R. , Mgaya Fauster X. , Kunambi Peter P. , Matee Mecky I.
Published: February 2023

Background

Previous studies have shown significant differences and lack clarity on whether resistance to either isoniazid or rifampicin can predict multidrug resistance tuberculosis (MDR-TB). Some consider rifampicin resistance to be a surrogate for MDR-TB. We, therefore, conducted this study to determine resistance to either isoniazid or rifampicin can predict MDR-TB.

Results

A total of 315 Mycobacteria tuberculosis isolates were tested for resistance against isoniazid, rifampicin, ethambutol and streptomycin using the phenotypic proportion method on Lowenstein–Jensen media. Results showed most isolates (88.9%, 280/315) were not resistant to any anti-TB tested, 5.7% (18/315) were resistant to both isoniazid and rifampicin, 2.5% (8/315) were resistant to rifampicin only and 1.3% (4/315) were resistant to all four first-line anti-tuberculosis. Prediction of MDR TB basing on rifampicin results showed sensitivity of 100.0%, specificity of 96.3%, diagnostic accuracy of 96.5%, and positive and negative predictive values of 62.1% and 100.0%, respectively. Isoniazid had sensitivity of 100.0%, specificity of 98.3%, diagnostic accuracy of 98.4%, and positive and negative predictive values of 78.3% and 100.0%, respectively. Prediction of rifampicin resistance based on isoniazid results had sensitivity of 62.1%, specificity of 98.3%, diagnostic accuracy of 94.9%, a positive predictive value of 78.3% and a negative predictive value of 96.2%.

Conclusions

Resistance to either rifampicin or isoniazid sub-optimally predicts MDR-TB. Despite having high sensitivity and specificity, the positive predictive value of rifampicin was only 62.1% and for isoniazid was 78.3%, suggesting that if either is tested in isolation both could result in false positives MDR-TB cases, resulting into patients being unnecessarily subjected to the more toxic and expensive second-line anti-TB drugs, which are less effective compared to first-line anti-TB drugs.
GNSS techniques for real-time monitoring of landslides: a review
Authors: Huang Guanwen , Du Shi , Wang Duo
Published: February 2023

Currently, Global Navigation Satellite System (GNSS) Real-Time Kinematic positioning (RTK) and Precise Point Positioning (PPP) techniques are widely employed for real-time monitoring of landslides. However, both RTK and PPP monitoring techniques have their limitations, such as limited service coverage or long convergence times. PPP-RTK technique which integrates RTK and PPP is a novel approach for monitoring landslides with the advantages of rapid convergence, high-precision, and a wide service area. This study summarizes the limitations of RTK, PPP, and PPP-RTK monitoring techniques and suggests some improved strategies. Their performances are compared and analyzed using real monitoring data. The experiment results demonstrate that RTK is the best option for small-scale (the baseline distance < 15 km) and real-time landslide monitoring without considering the cost. PPP technique converges to centimeter-level accuracy in tens of minutes, only suitable for the stability analysis of reference stations. Over a large area (the baseline distance < 100 km), PPP-RTK can provide excellent horizontal accuracy and adapt the service range in response to the demand for monitoring accuracy, as the vertical accuracy is significantly impacted by the service range and elevation difference. Finally, the characteristics of three techniques are integrated to form a comprehensive landslide monitoring technique that considers intelligence, robustness, and real-time.
Evaluation of protein C and S levels in patients with COVID-19 infection and their relation to disease severity
Authors: Elshafie Ahmed , Foda Enas , Yousef Mahmoud M. G. , El-Naby Kareem A. Abd
Published: February 2023

Background

The COVID-19 pandemic has been associated with millions of deaths around the world. One of the important causes of death associated with COVID-19 was pulmonary thromboembolism. The risk for venous thromboembolism was markedly increased in patients with COVID-19 especially those admitted to the intensive care unit. The aims of our study were to measure the protein C and S levels in COVID-19-infected patients in comparison with the normal population and to assess the correlation of protein C and S levels in the plasma to the severity of infection.

Methods

This was a case–control study measuring the protein C and S levels in patients infected with COVID-19 at the time of diagnosis compared to the normal population. The study included one hundred participants, sixty of them are patients with COVID-19, and forty of them are normal healthy adults. The patient group was subclassified into three subgroups according to disease severity: mild, moderate, and severe COVID-19 infections.

Results

The activity of protein C in the patient group serum was significantly lower than that in the control group serum (79.35 ± 26.017 vs 97.43 ± 15.007; p < 0.001). Protein S is also significantly decreased in patients’ serum when compared to the control group (70.233 ± 22.476 vs 91 ± 14.498; p < 0.001). There was a statistically significant decrease in the levels of protein C and S associated with the increase in disease severity (p < 0.05). However, protein S showed no statistically significant difference between the moderate and severe disease subgroups.

Conclusion

The study concluded that the levels of protein C and S activities were both decreased in patients with COVID-19 when compared to the healthy population. It also concluded that the decrease in their levels is statistically significant in relation to the disease severity.
Depositional and diagenetic controllers on the sandstone reservoir quality of the late cretaceous sediments, Gulf of Suez Basin
Authors: Kassem Ahmed A.
Published: February 2023

The complex depositional, burial, and diagenetic histories of the Late Cretaceous Nezzazat Group sandstones in Northeastern Africa present the main challenges with regard to reservoir quality. The quality of commercial reservoirs is maintained despite deep burial and the associated high temperature and pressure. The study presents an optimum integration of different data sets to address reservoir quality and reservoir performance controllers. The data set includes measured porosity and permeability, petrographic point-counting data, grain-size analysis, X-ray diffraction data, scanning electron microscopy, and porosity loss by compaction. The depositional controls on the reservoir quality are the facies, whereas the higher quality is found in the channel and upper shoreface settings. The coarse-grained sandstone is associated with better reservoir quality. The large intergranular porosity is the main porosity controlling the fluid flow. The massive and laminated sandstones are the best quality facies. The labile grains (feldspars and mica) control the permeability distribution. Whereas the secondary diagenetic controllers are the carbonate cementation that inhibited the effects of compaction. The siderite cementation has resulted in a micropore dominated and highly tortuous pore system. Total porosity has largely been preserved in the siderite-cemented sample but virtually eliminated in the dolomite cemented. A low volume of illite is associated with better reservoir quality. Whereas the better reservoir quality is associated with the abundant quartz cementation that protected the primary porosity from compaction. Compaction acts as a significant porosity loss factor during diagenesis. Authigenic kaolinite does not significantly affect the reservoir quality. The reservoir sensitivity to formation damage comes from the potential for fines (kaolinite, illitic clays, siderite, and pyrite) migration within the pore system that is readily mobilized by the fluid flow.
Association of self-reported mother–infant relationship with child and adolescent mental health
Authors: Rasmussen Ida Scheel , Wilson Philip , Overbeck Gritt , Strandberg-Larsen Katrine
Published: February 2023

Background

The quality of the relationship between mother and infant may have profound implications for the development of a child. Early indicators of psychological vulnerability may allow targeting of support for the child's cognitive, emotional and social development. A challenging mother–infant relationship could be one indicator of risk.

Aims

This study examined variations in psychological well-being and psychopathology among boys and girls according to early maternal perception of the mother–infant relationship.

Method

This study is based on 64 663 mother–infant pairs from the Danish National Birth Cohort, for which data on the mother–infant relationship were collected at 6 months postpartum. Behavioural problems were assessed with the Danish version of the Strengths and Difficulties Questionnaire (SDQ) at child ages 7, 11 and 18 years, and we retrieved information on diagnosed childhood and adolescent psychiatric disorders and prescriptions of psychotropic drugs from Danish registries.

Results

Children in the challenging mother–infant relationship group had higher odds of behavioural problems at age 7 among both boys and girls. The same pattern of elevated estimates was identified for boys across all SDQ domains and for girls in three of five SDQ domains. All associations were attenuated at age 18, but increased odds of behavioural problems still existed. A challenging early mother–infant relationship increased the offspring's risk of being diagnosed with a psychiatric disorder or being prescribed a psychotropic drug before the age of 18.

Conclusion

A challenging self-reported mother–infant relationship was associated with later psychopathological difficulties. Routine clinical enquiry may be useful in identification of future vulnerability.
NaCl 3% Bolus Therapy as Emergency Treatment for Severe Hyponatremia: Comparison of 100 mL vs 250 mL
Authors: Massop Karen , Haverkort Dieneke A , Bech Anneke P , de Boer Hans
Published: February 2023

Context

The aim of initial treatment of severe hyponatremia is to rapidly increase serum sodium to reduce the complications of cerebral edema. The optimal strategy to achieve this goal safely is still under debate.

Objective

To compare the efficacy and safety of 100 and 250 mL NaCl 3% rapid bolus therapy as initial treatment of severe hypotonic hyponatremia.

Methods

Retrospective analysis of patients admitted to a teaching hospital in The Netherlands between 2017 and 2019. The patients were 130 adults with severe hypotonic hyponatremia, defined as serum sodium ≤ 120 mmol/L. A bolus of either 100 mL (n = 63) or 250 mL (n = 67) NaCl 3% was the initial treatment. Successful treatment was defined as a rise in serum sodium ≥ 5 mmol/L within the first 4 hours after bolus therapy. Overcorrection of serum sodium was defined as an increase of more than 10 mmol/L in the first 24 hours.

Results

The percentage of patients with a rise in serum sodium ≥5 mmol/L within 4 hours was 32% and 52% after a bolus of 100 and 250 mL, respectively (P = .018). Overcorrection of serum sodium was observed after a median of 13 hours (range 9-17 hours) in 21% of patients in both treatment groups (P = .971). Osmotic demyelination syndrome did not occur.

Conclusion

Initial treatment of severe hypotonic hyponatremia is more effective with a NaCl 3% bolus of 250 mL than of 100 mL and does not increase the risk of overcorrection.
Postpartum Acute Aortic Dissection in a Patient Without a Connective Tissue Disorder: A Case Report
Authors: Meuli Mercades A. , Shapiro Anne , Cronin Brett H. , Pinson Kelsey A. , Martin Erin I.
Published: February 2023

Type A acute aortic dissection is a rare life-threatening event that occurs most commonly in the third trimester or early postpartum and in women with connective tissue disorders. However, this case describes a type A aortic dissection diagnosed on postpartum day 2 in a woman with preeclampsia without a history of a connective tissue disease. The case emphasizes the importance of considering dissection in any parturient complaining of chest pain, especially in the setting of hypertension and a new murmur. Emergent imaging must be considered to decrease delays in surgical repair and to minimize maternal morbidity and mortality.
Incidence and Risk Factor of Implant Dislocation After Cervical Disk Arthroplasty: A Retrospective Cohort Analysis of 756 Patients
Authors: Ko Chin-Chu , Feng Bo-Kai , Kuo Yi-Hsuan , Kuo Chao-Hung , Tu Tsung-Hsi , Chang Chih-Chang , Cheng Henrich , Huang Wen-Cheng , Wu Jau-Ching
Published: February 2023

BACKGROUND:

Implant dislocation after cervical disk arthroplasty (CDA) is obviously a critical complication, but no information about the incidence and associated risk factor has been reported.

OBJECTIVE:

To investigate the incidence and risk factor of implant dislocation after CDA by a retrospective cohort analysis.

METHODS:

A retrospective review of a consecutive series of CDA performed between January 2009 and March 2021 at a single institution was conducted. Analyses of chart records and radiological data established the incidence and associated risk factor of implant dislocation after CDA. A Kaplan-Meier survival estimation of implant survival was performed.

RESULTS:

A total of 756 consecutive patients were included in this analysis. Five patients (0.7%) had a migration and even dropout of the artificial disk. The overall cumulative survival rate of the implant reached approximately 99.3% of the 756 patients. Preoperative kyphosis was significantly related to implant dislocation (P = .016), with an odds ratio of 15.013.

CONCLUSION:

The incidence of implant dislocation after CDA is as low as 0.7% or 5/756 patients. Preoperative kyphosis significantly increases the risk of postoperative implant dislocation by a factor of 15. The migrating implants could be revealed on radiographs as early as 0.9 to 1.4 months postoperatively and were revised to anterior cervical diskectomy and fusion within half a year. No new event of implant dislocation occurred half a year postoperatively. The overall cumulative survival rate of the implant reached 99.3% of the 756 patients. In conclusion, CDA remains a safe and reliable procedure.
Tailored Hemispherotomy Using Tractography-Guided Laser Interstitial Thermal Therapy
Authors: Mendoza-Elias Nasya , Satzer David , Henry Julia , Nordli Douglas R. , Warnke Peter C.
Published: February 2023

BACKGROUND:

Medically refractory hemispheric epilepsy is a devastating disease with significant lifetime costs and social burden. Functional hemispherotomy is a highly effective treatment for hemispheric epilepsy but is associated with significant complication rates. Percutaneous hemispherotomy through laser interstitial thermal therapy (LITT) based on morphological MRI has been recently described in a single patient but not replicated in the literature.

OBJECTIVE:

To describe the first 2 cases of tractography-guided interstitial laser hemispherotomy and their short-term outcomes.

METHODS:

Two 11-year-old male patients with medically refractory epilepsy secondary to perinatal large vessel infarcts were referred for hemispherotomy. Both patients underwent multitrajectory LITT to disconnect the remaining pathological hemisphere, using tractography to define targets and assess structural outcomes.

RESULTS:

Both cases had minor complication of small intraventricular/subarachnoid hemorrhage not requiring additional intervention. Both patients remain seizure-free at all follow-up visits.

CONCLUSION:

LITT hemispherotomy can produce seizure freedom with short hospitalization and recovery. Tractography allows surgical planning to be tailored according to individual patient anatomy, which often is distorted in perinatal stroke. Minimally invasive procedures offer the greatest potential for seizure freedom without the risks of an open hemispherotomy.
Two phases for centripetal migration of Drosophila melanogaster follicle cells: initial ingression followed by epithelial migration
Authors: Parsons Travis T. , Mosallaei Sheila , Raftery Laurel A.
Published: February 2023

During Drosophila oogenesis, somatic follicle cells (FCs) differentiate to secrete components of the eggshell. Before secretion, the epithelium reorganizes to shape eggshell specializations, including border FC collective cell migration and later dorsal formation. These FC movements provide valuable insights into collective cell migration. However, little is known about centripetal migration, which encloses the oocyte after secretion has begun. Centripetal migration begins with apical extension of a few FCs that move away from the basement membrane to invade between germ cells. We define a timeline of reproducible milestones, using time-lapse imaging of egg chamber explants. Inward migration occurs in two phases. First, leading centripetal FCs ingress, extending apically over the anterior oocyte, and constricting basally. Second, following FCs move collectively toward the anterior, then around the corner to move inward with minimal change in aspect ratio. E-cadherin was required in leading centripetal FCs for their normal ingression, assessed with homozygous shotgun mutant or RNAi knockdown clones; ingression was influenced non-autonomously by mutant following FCs. This work establishes centripetal migration as an accessible model for biphasic E-cadherin-adhesion-mediated collective migration.
The force-dependent filamin A–G3BP1 interaction regulates phase-separated stress granule formation
Authors: Feng Ziyi , Mao Zhenfeng , Yang Ziwei , Liu Xiaowei , Nakamura Fumihiko
Published: February 2023

Filamin A (FLNA) is an actin crosslinking protein that mediates mechanotransduction. External and internal mechanical forces, through the actin cytoskeleton, can induce conformational changes of the FLNA molecule to expose cryptic binding sites for its binding partners. Here, we identified Ras GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) as a new FLNA mechanobinding partner. Unlike other FLNA binding partners to the mechanosensing domain repeat 21 (R21), G3BP1 requires an additional neighboring repeat R22 to interact. We demonstrated that their interaction occurs in the cytosol of living cells in an actin polymerization-dependent manner. We also mapped the FLNA-binding site on G3BP1 and found that a F360A point mutation in the RNA recognition motif disrupts the interaction. RNA interfered with the FLNA–G3BP1 interaction, and FLNA did not localize in RNA-rich stress granules (SGs). Disruption of the interaction was sufficient to promote phase-separated SG formation, and arsenite treatment further stimulated the formation of SGs. Taken together, these data identify G3BP1 as a new mechanobinding protein that interacts with the FLNA mechanosensing domain R21 and suggest that SG formation is partially regulated by mechanical force.