bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual Role for FHY3 in Light Input to the Clock
Authors: Rhodes Bruce M. , Siddiqui Hamad , Khan Safina , Devlin Paul F.
Published: June 2022

The red-light regulated transcription factors FHY3 and FAR1 form a key point of light input to the plant circadian clock in positively regulating expression of genes within the central clock. However, the fhy3 mutant shows an additional red light-specific disruption of rhythmicity which is inconsistent with this role. Here we demonstrate that only fhy3 and not far1 mutants show this red specific disruption of rhythmicity. We examined the differences in rhythmic transcriptome in red versus white light and reveal differences in patterns of rhythmicity among the central clock proteins suggestive of a change in emphasis within the central mechanism of the clock, changes which underlie the red specificity of the fhy3 mutant. In particular, changes in enrichment of promoter elements were consistent with a key role for the HY5 transcription factor, a known integrator of the ratio of red to blue light in regulation of the clock. Examination of differences in the rhythmic transcriptome in the fhy3 mutant in red light identified specific disruption of the CCA1-regulated ELF3 and LUX central clock genes, while the CCA1 target TBS element, TGGGCC, was enriched among genes that became arrhythmic. Coupled with the known interaction of FHY3 but not FAR1 with CCA1 we propose that the red-specific circadian phenotype of fhy3 may involve disruption of the previously demonstrated moderation of CCA1 activity by FHY3 rather than a disruption of its own transcriptional regulatory activity. Together, this evidence suggests a conditional redundancy between FHY3 and HY5 in the integration of red and blue light input to the clock in order to enable a plasticity in response to light and optimise plant adaptation. Furthermore, our evidence also suggests changes in CCA1 activity between red and white light transcriptomes. This, together with the documented interaction of HY5 with CCA1, leads us to propose a model whereby this integration of red and blue signals may at least partly occur via direct FHY3 and HY5 interaction with CCA1 leading to moderation of CCA1 activity.
Exploring the SiCCT Gene Family and Its Role in Heading Date in Foxtail Millet
Authors: Li Congcong , Ma Jian , Wang Genping , Li Haiquan , Wang Hailong , Wang Guoliang , Jiang Yanmiao , Liu Yanan , Liu Guiming , Liu Guoqing , Cheng Ruhong , Wang Huan , Wei Jianhua , Yao Lei
Published: June 2022

CCT transcription factors are involved in the regulation of photoperiod and abiotic stress in Arabidopsis and rice. It is not clear that how CCT gene family expand and regulate heading date in foxtail millet. In this study, we conducted a systematic analysis of the CCT gene family in foxtail millet. Thirty-nine CCT genes were identified and divided into four subfamilies based on functional motifs. Analysis showed that dispersed duplication played a predominant role in the expansion of CCT genes during evolution. Nucleotide diversity analysis suggested that genes in CONSTANS (COL)-like, CCT MOTIF FAMILY (CMF)-like, and pseudoresponse response regulator (PRR)-like subfamilies were subjected to selection. Fifteen CCT genes were colocalized with previous heading date quantitative trait loci (QTL) and genome-wide association analysis (GWAS) signals. Transgenic plants were then employed to confirm that overexpression of the CCT gene SiPRR37 delayed the heading date and increased plant height. Our study first investigated the characterization and expansion of the CCT family in foxtail millet and demonstrated the role of SiPRR37. These results lay a significant foundation for further research on the function of CCT genes and provide a cue for the regulation of heading date.
Multi-Omics Approaches Unravel Specific Features of Embryo and Endosperm in Rice Seed Germination
Authors: Sano Naoto , Lounifi Imen , Cueff Gwendal , Collet Boris , Clément Gilles , Balzergue Sandrine , Huguet Stéphanie , Valot Benoît , Galland Marc , Rajjou Loïc
Published: June 2022

Seed germination and subsequent seedling growth affect the final yield and quality of the crop. Seed germination is defined as a series of processes that begins with water uptake by a quiescent dry seed and ends with the elongation of embryonic axis. Rice is an important cereal crop species, and during seed germination, two tissues function in a different manner; the embryo grows into a seedling as the next generation and the endosperm is responsible for nutritional supply. Toward understanding the integrated roles of each tissue at the transcriptional, translational, and metabolic production levels during germination, an exhaustive “multi-omics” analysis was performed by combining transcriptomics, label-free shotgun proteomics, and metabolomics on rice germinating embryo and endosperm, independently. Time-course analyses of the transcriptome and metabolome in germinating seeds revealed a major turning point in the early phase of germination in both embryo and endosperm, suggesting that dramatic changes begin immediately after water imbibition in the rice germination program at least at the mRNA and metabolite levels. In endosperm, protein profiles mostly showed abundant decreases corresponding to 90% of the differentially accumulated proteins. An ontological classification revealed the shift from the maturation to the germination process where over-represented classes belonged to embryonic development and cellular amino acid biosynthetic processes. In the embryo, 19% of the detected proteins are differentially accumulated during germination. Stress response, carbohydrate, fatty acid metabolism, and transport are the main functional classes representing embryo proteome change. Moreover, proteins specific to the germinated state were detected by both transcriptomic and proteomic approaches and a major change in the network operating during rice germination was uncovered. In particular, concomitant changes of hormonal metabolism-related proteins (GID1L2 and CNX1) implicated in GAs and ABA metabolism, signaling proteins, and protein turnover events emphasized the importance of such biological networks in rice seeds. Using metabolomics, we highlighted the importance of an energetic supply in rice seeds during germination. In both embryo and endosperm, starch degradation, glycolysis, and subsequent pathways related to these cascades, such as the aspartate-family pathway, are activated during germination. A relevant number of accumulated proteins and metabolites, especially in embryos, testifies the pivotal role of energetic supply in the preparation of plant growth. This article summarizes the key genetic pathways in embryo and endosperm during rice seed germination at the transcriptional, translational, and metabolite levels and thereby, emphasizes the value of combined multi-omics approaches to uncover the specific feature of tissues during germination.
Asymmetric Interaction Between Two Mycorrhizal Fungal Guilds and Consequences for the Establishment of Their Host Plants
Authors: Fernández Natalia , Knoblochová Tereza , Kohout Petr , Janoušková Martina , Cajthaml Tomáš , Frouz Jan , Rydlová Jana
Published: June 2022

Arbuscular mycorrhiza (AM) and ectomycorrhiza (EcM) are the most abundant and widespread types of mycorrhizal symbiosis, but there is little and sometimes conflicting information regarding the interaction between AM fungi (AMF) and EcM fungi (EcMF) in soils. Their competition for resources can be particularly relevant in successional ecosystems, which usually present a transition from AM-forming herbaceous vegetation to EcM-forming woody species. The aims of this study were to describe the interaction between mycorrhizal fungal communities associated with AM and EcM hosts naturally coexisting during primary succession on spoil banks and to evaluate how this interaction affects growth and mycorrhizal colonization of seedlings of both species. We conducted a greenhouse microcosm experiment with Betula pendula and Hieracium caespitosum as EcM and AM hosts, respectively. They were cultivated in three-compartment rhizoboxes. Two lateral compartments contained different combinations of both host plants as sources of fungal mycelia colonizing the middle compartment, where fungal biomass, diversity, and community composition as well as the growth of each host plant species’ seedlings were analyzed. The study’s main finding was an asymmetric outcome of the interaction between the two plant species: while H. caespitosum and associated AMF reduced the abundance of EcMF in soil, modified the composition of EcMF communities, and also tended to decrease growth and mycorrhizal colonization of B. pendula seedlings, the EcM host did not have such effects on AM plants and associated AMF. In the context of primary succession, these findings suggest that ruderal AM hosts could hinder the development of EcM tree seedlings, thus slowing the transition from AM-dominated to EcM-dominated vegetation in early successional stages.
Fulvic Acid Alleviates Paper Sludge Toxicity in Canola (Brassica napus L.) by Reducing Cr, Cd, and Pb Uptake
Authors: Khilji Sheza Ayaz , Sajid Zahoor Ahmad , Fayyaz Sidra , Shah Anis Ali , Shah Adnan Noor , Rauf Mamoona , Arif Muhammad , Yang Seung Hwan , Fiaz Sajid
Published: June 2022

Heavy metal toxicity reduces the growth and development of crop plants growing in metal-contaminated regions. Disposal of industrial waste in agricultural areas has negative effects on the physiochemical activities of plants. This research aimed to examine the fulvic acid (FA)-mediated efficacy of Brassica napus L. regarding stress tolerance in soil amended with paper sludge (PS). For this purpose, plants were grown for 90 days under greenhouse conditions at various concentrations of PS-amended soils (0, 5, 10, and 15%) being irrigated with water containing FA (0, 10, and 20%). All the physicochemical parameters of PS were carried out before and after plant transplantation. Paper sludge toxicity reduced the growth (shoot/root length, fresh/dry weight of shoot/root, numbers of flowers and leaves) and physicochemical characteristics of exposed B. napus plants. In comparison, FA application improved growth by reducing the metal uptake in the shoot of plants grown at various concentrations of PS. An increasing trend in antioxidant enzyme activity was observed by increasing the FA concentration (0%-10% and 20%). Post-harvest analysis indicated that the amount of tested metals was significantly reduced at all PS concentrations. Minimum metal uptake was observed at 0% concentration and maximum at 15% concentration of paper sludge. Additionally, FA application at 20% concentration reduced Chromium (Cr), Cadmium (Cd), and Lead (Pb) uptake in the shoot from 6.08, 34.42, and 20.6 mgkg−1 to 3.62, 17.33, and 15.22 mgkg−1, respectively. At this concentration of paper sludge in the root, 20% FA reduced Cr, Cd, and Pb uptake from 11.19, 44.11, and 35.5 mgkg−1 to 7.88, 27.01, and 24.02 mgkg−1, respectively. Thus, FA at 20% concentration was found to be an effective stimulant to mitigate the metal stress in B. napus grown in paper sludge-polluted soil by reducing metal uptake and translocation to various plant parts.
Stone Wool Substrate Cover Incision Impacts on the Root-Zone Water Content, Temperature, and Yield of Tomato Cultures
Authors: Ahn Tae In , Yang Jung-Seok , Im Yong-Hoon , Youn Young Jik , Lee Ju Young
Published: June 2022

Standardized cultivation systems are crucial for establishing reproducible agronomic techniques. Especially stone wool-based cultivation is governed by standardized specifications and provides a controllable root-zone environment. However, the effects of stone wool cover incision on root-zone variability have rarely been studied. Therefore, in this study, we focused on the effect of the stone wool cover incision method on environmental variations and their subsequent effects on tomato plant productivity. Stone wool slab plastic covers represent a core component of this substrate system that can potentially affect the performance of water control techniques. We designed a cover incision method to create four different levels of drainage performances that were tested by cultivating tomato plants (Solanum lycopersicum “Dafnis”). The water content, root-zone temperature, and dissolved oxygen were measured and analyzed relative to the tomato yield. We found that the incision level with the lowest drainage performance showed a lower air-root zone temperature correlation slope than those of slabs with favorable drainage conditions. Furthermore, these slabs had low dissolved oxygen levels (3.2 mg/L); nevertheless, the tomatoes grown in the slabs with incision level showing the lowest drainage performance had greater fruit yield (6,748 g/plant) than those in the slabs with favorable drainage conditions (6,160 g/plant). Furthermore, the normalized yield separation timing between treatments coincided with the hotter air temperature (27°C average) periods. We noted that manipulating the cover incision process consequently entailed variations in the correlation slope between the air temperature and root-zone temperature in the substrate. Our results reveal another trade-off relationship in the conventional perspective on the drainage performance effects and provide insights into further optimization of crop production and water use in the stone wool-based system.
Light Quality Modulates Plant Cold Response and Freezing Tolerance
Authors: Kameniarová Michaela , Černý Martin , Novák Jan , Ondrisková Vladěna , Hrušková Lenka , Berka Miroslav , Vankova Radomira , Brzobohatý Bretislav
Published: June 2022

The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in 1-month-old Arabidopsis thaliana (Col-0) plants exposed for 1 week to 4°C at short-day conditions under white (100 and 20 μmol m−2s−1), blue, or red (20 μmol m−2s−1) light conditions. An upregulated expression of CBF1, inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in Arabidopsis accessions originating from different and contrasting latitudes. In all but one Arabidopsis accession, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light-treated plants at low temperatures and showed that the cold response is highly accession-specific. In general, blue light increased mainly the cold-stress-related proteins and red light-induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light-driven cell function maintaining program and blue light-activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.
Supplemental Blue Light Frequencies Improve Ripening and Nutritional Qualities of Tomato Fruits
Authors: He Rui , Wei Jingjing , Zhang Jiye , Tan Xin , Li Yamin , Gao Meifang , Liu Houcheng
Published: June 2022

Tomatoes (Solanum lycopersicum L. Micro-Tom) were grown in a plastic greenhouse. When plants anthesis, the 100 μmol m–2 s–1 blue light-emitting diode (LED) light (430 ± 10 nm) was supplemented from 6:00 to 18:00. There were 5 treatments, which contained different blue light frequencies with the same intensity: S6 (30 min blue light and 30 min pause), S8 (30 min blue light and 15 min pause), S10 (30 min blue and 8 min pause), S12 (continuous blue light for 12 h), and control (CK) (natural light, without any supplemental light). Agronomic traits and nutritional qualities of tomato fruits were measured at 30, 34, 38, 42, and 46 days after anthesis (DAA), respectively. Different frequencies of supplemental blue light could accelerate flowering of tomato plants and promote fruit ripening about 3–4 days early via promoting ethylene evolution of fruits, which significantly facilitated the processes of color change and maturity in tomato fruits. The contents of lycopene, total phenolic compounds, total flavonoids, vitamin C, and soluble sugar, as well as the overall antioxidant activity of tomato fruits were significantly enhanced by all the supplemental blue light treatments. In all, different frequencies of supplemental blue light prominently reinforced the antioxidant levels and nutritional qualities of tomato fruits, especially lycopene content, and S10 was more optimal for tomato fruits production in a plastic greenhouse.
MiR395c Regulates Secondary Xylem Development Through Sulfate Metabolism in Poplar
Authors: Liu Chunhao , Ma Ding , Wang Zihao , Chen Ningcong , Ma Xiaoyun , He Xin-Qiang
Published: June 2022

Secondary xylem development requires the coordination of multiple regulatory factors, including plant hormones, transcription factors, and microRNAs (miRNAs). MiR395 is an important regulator involved in sulfate metabolism, but its function in plant development is unclear. This study investigated the functions of miR395c in the secondary xylem development in Populus alba × P. glandulosa. MiR395c was highly expressed in the shoot apex and secondary xylem. The overexpression of miR395c resulted in an increase in both secondary xylem width and vessel dimension, as well as a decrease in the thickness of the secondary cell wall of the xylem fiber. Further analysis showed that miR395c inhibited biosynthesis of sulfate metabolic products by targeting ATPS genes, which led to the reduction of Abscisic acid (ABA) synthesis and down-regulation of MYB46 expression. Our results indicate that miR395c regulates the secondary xylem development process via sulfate metabolism in Populus.
An Improved Method for Monitoring Multiscale Plant Species Diversity of Alpine Grassland Using UAV: A Case Study in the Source Region of the Yellow River, China
Authors: Sun Yi , Yuan Yaxin , Luo Yifei , Ji Wenxiang , Bian Qingyao , Zhu Zequn , Wang Jingru , Qin Yu , He Xiong Zhao , Li Meng , Yi Shuhua
Published: June 2022

Plant species diversity (PSD) is essential in evaluating the function and developing the management and conservation strategies of grassland. However, over a large region, an efficient and high precision method to monitor multiscale PSD (α-, β-, and γ-diversity) is lacking. In this study, we proposed and improved an unmanned aerial vehicle (UAV)-based PSD monitoring method (UAVB) and tested the feasibility, and meanwhile, explored the potential relationship between multiscale PSD and precipitation on the alpine grassland of the source region of the Yellow River (SRYR), China. Our findings showed that: (1) UAVB was more representative (larger monitoring areas and more species identified with higher α- and γ-diversity) than the traditional ground-based monitoring method, though a few specific species (small in size) were difficult to identify; (2) UAVB is suitable for monitoring the multiscale PSD over a large region (the SRYR in this study), and the improvement by weighing the dominance of species improved the precision of α-diversity (higher R2 and lower P values of the linear regressions); and (3) the species diversity indices (α- and β-diversity) increased first and then they tended to be stable with the increase of precipitation in SRYR. These findings conclude that UAVB is suitable for monitoring multiscale PSD of an alpine grassland community over a large region, which will be useful for revealing the relationship of diversity–function, and helpful for conservation and sustainable management of the alpine grassland.
Prediction of Phenolic Contents Based on Ultraviolet-B Radiation in Three-Dimensional Structure of Kale Leaves
Authors: Yoon Hyo In , Kim Jaewoo , Oh Myung-Min , Son Jung Eek
Published: June 2022

Ultraviolet-B (UV-B, 280–315 nm) radiation has been known as an elicitor to enhance bioactive compound contents in plants. However, unpredictable yield is an obstacle to the application of UV-B radiation to controlled environments such as plant factories. A typical three-dimensional (3D) plant structure causes uneven UV-B exposure with leaf position and age-dependent sensitivity to UV-B radiation. The purpose of this study was to develop a model for predicting phenolic accumulation in kale (Brassica oleracea L. var. acephala) according to UV-B radiation interception and growth stage. The plants grown under a plant factory module were exposed to UV-B radiation from UV-B light-emitting diodes with a peak at 310 nm for 6 or 12 h at 23, 30, and 38 days after transplanting. The spatial distribution of UV-B radiation interception in the plants was quantified using ray-tracing simulation with a 3D-scanned plant model. Total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), UV-B absorbing pigment content (UAPC), and the antioxidant capacity were significantly higher in UV-B-exposed leaves. Daily UV-B energy absorbed by leaves and developmental age was used to develop stepwise multiple linear regression models for the TPC, TFC, TAC, and UAPC at each growth stage. The newly developed models accurately predicted the TPC, TFC, TAC, and UAPC in individual leaves with R2 > 0.78 and normalized root mean squared errors of approximately 30% in test data, across the three growth stages. The UV-B energy yields for TPC, TFC, and TAC were the highest in the intermediate leaves, while those for UAPC were the highest in young leaves at the last stage. To the best of our knowledge, this study proposed the first statistical models for estimating UV-B-induced phenolic contents in plant structure. These results provided the fundamental data and models required for the optimization process. This approach can save the experimental time and cost required to optimize the control of UV-B radiation.
Phylogenomics and Genetic Diversity of Arnebiae Radix and Its Allies (Arnebia, Boraginaceae) in China
Authors: Sun Jiahui , Wang Sheng , Wang Yiheng , Wang Ruishan , Liu Kangjia , Li Enze , Qiao Ping , Shi Linyuan , Dong Wenpan , Huang Luqi , Guo Lanping
Published: June 2022

Arnebiae Radix is a traditional medicine with pleiotropic properties that has been used for several 100 years. There are five species of Arnebia in China, and the two species Arnebia euchroma and Arnebia guttata are the source plants of Arnebiae Radix according to the Chinese Pharmacopoeia. Molecular markers that permit species identification and facilitate studies of the genetic diversity and divergence of the wild populations of these two source plants have not yet been developed. Here, we sequenced the chloroplast genomes of 56 samples of five Arnebia species using genome skimming methods. The Arnebia chloroplast genomes exhibited quadripartite structures with lengths from 149,539 and 152,040 bp. Three variable markers (rps16-trnQ, ndhF-rpl32, and ycf1b) were identified, and these markers exhibited more variable sites than universal chloroplast markers. The phylogenetic relationships among the five Arnebia species were completely resolved using the whole chloroplast genome sequences. Arnebia arose during the Oligocene and diversified in the middle Miocene; this coincided with two geological events during the late Oligocene and early Miocene: warming and the progressive uplift of Tianshan and the Himalayas. Our analyses revealed that A. euchroma and A. guttata have high levels of genetic diversity and comprise two and three subclades, respectively. The two clades of A. euchroma exhibited significant genetic differences and diverged at 10.18 Ma in the middle Miocene. Three clades of A. guttata diverged in the Pleistocene. The results provided new insight into evolutionary history of Arnebia species and promoted the conservation and exploitation of A. euchroma and A. guttata.
Wheat Escapes Low Light Stress by Altering Pollination Types
Authors: Yang Hong , Li Yongpeng , Li Dongxiao , Liu Liantao , Qiao Yunzhou , Sun Hongyong , Liu Wenwen , Qiao Wenjun , Ma Yuzhao , Liu Mengyu , Li Cundong , Dong Baodi
Published: June 2022

Although low light stress seriously affects florets fertility and grain number during the reproductive period, crops can be fertilized by heterologous pollen to alleviate the reduction of grain number. However, wheat is strongly autogamous, how to change to outcross after low light remains unclear. To understand the mechanisms of this change process, an approach combined morphological, physiological, and transcriptomic analyses was performed under low light stress imposed at the young microspore stage the booting stage from tetrad to uni-nucleate microspores stage. The results showed that low light stress caused pollen abortion, and the unfertilized ovary is fertilized by heterologous pollen after floret opening. Compared to control, the opening angle of lemma and glume were increased by 11.6–48.6 and 48.4–78.5%, respectively. The outcross of stressed wheat compensated for the 2.1–18.0% of grain number loss. During this process, phytohormones played an important role. Jasmonic acid (JA) and methyl jasmonate (MeJA) levels in spikelets were increased. Meanwhile, lignin and cellulose content decreased, and genes associated with cell wall related GO terms were enriched. Among the differentially expressed genes (DEGs), were identified 88-710 transcription factors genes, of which some homologs in Arabidopsis are proposed to function in lignin and cellulose, influencing the glume and lemma opening. Our finding can provide new insight into a survival mechanism to set seeds through pollination way alteration in the absence of self-fertilization after the stress of adversity.
Reading the Wind: Impacts of Leader Negative Emotional Expression on Employee Silence
Authors: Chen Shu-Chen , Shao Jieqi , Liu Na-Ting , Du Yu-Shan
Published: June 2022

Employee silence has multiple negative effects on the organization. Studies on the influence of leader negative emotional expression on employee silence are extremely limited, and there are inconsistent findings for the expression of negative emotion among leaders, which highlight the need to explore boundary factors in this field. The purpose of this paper is based on EASI model to examine the impact of leaders’ negative emotional expression on employee silence through the perceptions of psychological safety. Moreover, drawing on social exchange theory, this paper proposed a moderated mediation model to explore how leader–member exchange (LMX) moderates the indirect relationship between leader negative emotional expression and employee silence through perceptions of psychological safety. We employed a bootstrapping technique to analyze the hypotheses. This study adopts two-wave surveys and the results shown that leader negative emotional expression triggered employee silence by employees’ perceptions of psychological safety. This study also demonstrated that LMX weakens the relationship between leader negative emotional expression and employees’ perceptions of psychological safety. Furthermore, LMX weakens the indirect relationship between leader negative emotional expression and employee silence through employees’ perceptions of psychological safety. Using multiphase data collection, we found that when LMX is at a low level, the indirect effect of leader negative emotional expression on employee silence through employee psychological safety is stronger. The theoretical, practical implications and future research suggestions are discussed.
Interpersonal Emotion Regulation: Consequences for Brands in Customer Service Interactions
Authors: Reeck Crystal , Onuklu N. Nur Yazgan
Published: June 2022

This research demonstrates that interpersonal emotion regulation—attempts to manage others’ feelings—influences consumer perceptions during sales and service interactions impacting brand trust and loyalty. Building on previous research linking interpersonal emotion regulation to improved outcomes between people, across five experiments, we demonstrate that antecedent-focused interpersonal emotion regulation strategies result in enhanced brand loyalty and brand trust compared to response-focused interpersonal emotion regulation strategies. Analysis of mediation models reveals this effect is explained by changes in the consumer’s emotions, which in turn influence evaluations of the service interaction and subsequently impacts brand outcomes. We identify reactance as a moderator of this effect, such that customers with low (high) reactance to interpersonal regulation attempts exhibit more (less) favorable brand trust and loyalty evaluations. Further, we demonstrate that the visibility of interpersonal emotion regulation represents an important boundary condition. These findings support the process model of interpersonal emotion regulation and generate important insights for both theory and practice.
A Radial Basis Function Neural Network Approach to Predict Preschool Teachers’ Technology Acceptance Behavior
Authors: Rad Dana , Magulod Gilbert C. , Balas Evelina , Roman Alina , Egerau Anca , Maier Roxana , Ignat Sonia , Dughi Tiberiu , Balas Valentina , Demeter Edgar , Rad Gavril , Chis Roxana
Published: June 2022

With the continual development of artificial intelligence and smart computing in recent years, quantitative approaches have become increasingly popular as an efficient modeling tool as they do not necessitate complicated mathematical models. Many nations have taken steps, such as transitioning to online schooling, to decrease the harm caused by coronaviruses. Inspired by the demand for technology in early education, the present research uses a radial basis function (RBF) neural network (NN) modeling technique to predict preschool instructors’ technology usage in classes based on recognized determinant characteristics of technology acceptance. In this regard, this study utilized the RBFNN approach to predict preschool teachers’ technology acceptance behavior, based on the theory of planned behavior, which states that behavioral achievement, in our case the actual technology use in class, depends on motivation, intention and ability, and behavioral control. Thus, this research design is based on an adapted version of the technology acceptance model (TAM) with eight dimensions: D1. Perceived usefulness, D2. Perceived ease of use, D3. Perceived enjoyment, D4. Intention to use, D5. Actual use, D6. Compatibility, D7. Attitude, and D8. Self-efficacy. According to the TAM, actual usage is significantly predicted by the other seven dimensions used in this research. Instead of using the classical multiple linear regression statistical processing of data, we opted for a NN based on the RBF approach to predict the actual usage behavior. This study included 182 preschool teachers who were randomly chosen from a project-based national preschool teacher training program and who responded to our online questionnaire. After designing the RBF function with the actual usage as an output variable and the other seven dimensions as input variables, in the model summary, we obtained in the training sample a sum of squares error of 37.5 and a percent of incorrect predictions of 43.3%. In the testing sample, we obtained a sum of squares error of 14.88 and a percent of incorrect predictions of 37%. Thus, we can conclude that 63% of the classified data are correctly assigned to the models’ dependent variable, i.e., actual technology use, which is a significant rate of correct predictions in the testing sample. This high significant percentage of correct classification represents an important result, mainly because this is the first study to apply RBFNN’s prediction on psychological data, opening up a new interdisciplinary field of research.
Unpacking the Development of Chinese Preservice English as a Foreign Language Teachers’ Professional Knowledge
Authors: Liu Liyan , Jiang Anne Li , Yang Shiyu , Li Shuo
Published: June 2022

Efforts to improve preservice teacher education have recently focused on developing teachers’ adequate pedagogical knowledge (PK), content knowledge (CK), and pedagogical content knowledge (PCK), which are critical elements of teacher’s professional knowledge, and important indicators of preparedness to teach. However, the development of the three knowledge domains of Chinese preservice English as a foreign language (EFL) teachers is surprisingly under-researched. To fill this gap, this study examined the development of the three knowledge domains of a group of Chinese preservice EFL teachers at different stages of a teacher education program. Specifically, it explored the relationship among the three knowledge domains, and the effects of learning opportunities on their development. Findings revealed that preservice EFL teachers at a later stage outperformed those at an earlier stage with regard to PK and PCK. Our findings also suggested that there were positive correlations among PK, CK, and PCK at different stages of the teacher education program. Furthermore, the findings showed that courses on CK, PK, and PCK, and teaching experience significantly influenced preservice EFL teachers’ professional knowledge. However, the role of classroom observation was not significant. Implications for EFL teacher education and future research were also discussed.
Art and Perception: Using Empirical Aesthetics in Research on Consciousness
Authors: Ansorge Ulrich , Pelowski Matthew , Quigley Cliodhna , Peschl Markus F. , Leder Helmut
Published: June 2022

Understanding consciousness is a major frontier in the natural sciences. However, given the nuanced and ambiguous sets of conditions regarding how and when consciousness appears to manifest, it is also one of the most elusive topics for investigation. In this context, we argue that research in empirical aesthetics—specifically on the experience of art—holds strong potential for this research area. We suggest that empirical aesthetics of art provides a more exhaustive description of conscious perception than standard laboratory studies or investigations of the less artificial, more ecological perceptual conditions that dominate this research, leading to novel and better suited designs for natural science research on consciousness. Specifically, we discuss whether empirical aesthetics of art could be used for a more adequate picture of an observer’s attributions in the context of conscious perception. We point out that attributions in the course of conscious perception to (distal) objects versus to media (proximal objects) as origins of the contents of consciousness are typically swift and automatic. However, unconventional or novel object-media relations used in art can bring these attributions to the foreground of the observer’s conscious reflection. This is the reason that art may be ideally suited to study human attributions in conscious perception compared to protocols dedicated only to the most common and conventional perceptual abilities observed under standard laboratory or “natural”/ecological conditions alone. We also conclude that art provides an enormous stock of such unconventional and novel object-media relations, allowing more systematic falsification of tentative conclusions about conscious perception versus research protocols covering more conventional (ecological) perception only. We end with an outline of how this research could be carried out in general.
The Influence of College Students’ Innovation and Entrepreneurship Intention in the Art Field of Art Film and Television Appreciation by Deep Learning Under Entrepreneurial Psychology
Authors: Wang Minxin , Shao Yefan , Fu Shiman , Ye Lele , Li Hongming , Yang Guodong
Published: June 2022

There are many films and televisions (FATs) on the Internet, but the quality is uneven. This study explores the ability of college students to screen good films and resist bad films in television works in such a large environment. In the deep learning model of FAT, the ability of college students to think about the ideas expressed and the degree of influence on college students’ values are analyzed. Based on this conceptual basis, a questionnaire is designed for the intention and influencing factors of college students’ FAT innovation and entrepreneurship. It reflects the influence of concentration on FAT learning, the cognitive level of deep learning, the ability to process deep learning ideas, the feeling of the teaching process, and the process of self-learning, which all positively impact college students’ FAT entrepreneurial intentions. The importance of innovative deep learning is highlighted, which proves that a good deep learning course guidance method can improve students’ interest and ability and provide a reference for relevant colleges and universities to cultivate pertinent talents of the field of FAT.
Can Digit Ratio and Gender Identity Predict Preferences for Consumption Options With a Distinct Gender Image?
Authors: Otterbring Tobias , Elbæk Christian T. , Lu Chaoren
Published: June 2022

This study investigated whether individuals’ preferences for masculine (vs. feminine) consumption options could be predicted by a biological sex cue (the 2D:4D digit ratio; a biomarker linked to prenatal testosterone exposure), and a psychological gender cue (self-perceived gender identity). Chinese participants (N= 216) indicated their preferences for a series of binary options that differed in their perceived gender image (e.g., romantic comedy vs. action thriller; pop music vs. hard rock), with one of the options evaluated as relatively more feminine and the other viewed as comparably more masculine. Participants also self-reported their gender identity and the length of their index and ring fingers, which was used to calculate their 2D:4D digit ratios. A low (male-typical) digit ratio and a masculine gender identity were both associated with more masculine preferences, regardless of participants’ biological sex. However, a low digit ratio predicted preferences for masculine consumption options only in female participants with a masculine gender identity, but not in those with a feminine gender identity. These findings add to the literature on whether and when biological sex cues and psychological gender cues can predict preferences for options with a distinct gender image and suggest that the connection between these cues is more complex in women than in men.
Evaluating the Change Process of a Brief Cognitive Behavior Therapy Workshop for Psychological Distress Among Primary Care Self-Referrals in Selangor, Malaysia
Authors: Sam Jeng Mun , Ismail Siti Irma Fadhilah , Tan Kit-Aun , Sidik Sherina Mohd , Osman Zubaidah Jamil
Published: June 2022

Despite the high prevalence of psychological distress in primary care, only a limited number of individuals can benefit from early and evidenced-based psychological approach. Barriers to help-seeking attributable to stigma, lack of proper care pathway to evidence-based psychological interventions, and a high volume of primary care attendees are among the factors that contribute to the inadequate psychological treatments. This study examined the implementation of a change process using a brief cognitive behavioral therapy (b-CBT) workshop as a potential approach in managing psychological distress among 73 primary care self-referrals using purposive sampling. One-way repeated-measures multivariate analysis of variance (ANOVA) was used to analyze changes in psychological distress within a non-randomized quasi-experimental study. Results revealed significant differences in psychological distress using Depression, Anxiety, and Stress Scale-21 items at three time points (pre-intervention, post-intervention, and 1-month follow-up). Implementation of the b-CBT workshop showed positive changes for psychological distress, suggesting the possibility of integrating brief, non-stigmatized, and evidence-based psychological approach at the primary care level. However, the self-referral characteristics of the attendees remain unknown. Factors such as potential feasibility, participant's usability and satisfaction, and implementation of b-CBT workshop to improve psychological distress are discussed in this study.
School-Based Mental Health Initiative: Potentials and Challenges for Child and Adolescent Mental Health
Authors: Lai Kelly Y. C. , Hung Se-Fong , Lee Hannah W. S. , Leung Patrick W. L.
Published: June 2022

School-based mental health support services allow children and adolescents easy access to services without requirement of traveling to clinics and hospitals. We describe a School Mental Health Support Scheme (SMHSS) piloted in Hong Kong and discuss the challenges and learnings from the experience. This conceptual paper argues that accessibility is not the only advantage of such services. Teachers are significant others in child development, alongside with families. They play a central role in impacting the children's/adolescents' needs for competence and adult attachment, while schools provide an expanded social network of peers for one's social relationship. The fulfillment of these needs has powerful implications in the mental health of the children/adolescents. Teachers can help students to develop a sense of competence with self-worth and self-identity via providing guidance and feedback, whether they be on one's strengths or weaknesses, with acceptance, tolerance and unconditional positive regard. Particularly, the latter define a form of teacher-student relationship or adult attachment that offers the children/adolescents emotional security and nourishment, protecting them from failings and adversities. Teachers can also supervise and guide their students' social development with peers at schools. A recent meta-analysis has found preliminary evidence that those school-based mental health services integrated into the teachers' routine teaching activities are more effective. Teachers, who are overworked and stressed by the schools' overemphasis on academics and grades, have yet to fully grasp their unique roles in supporting students with mental health needs. This paper ends by advocating a paradigm shift in which both the healthcare professionals and educators should forge a mutually beneficial collaboration in jointly enhancing the mental health of children/adolescents at schools.
Correlation Between Sialidase NEU1 mRNA Expression Changes in Autism Spectrum Disorder
Authors: Zhang Haiqing , Gu Yuhang , He Wenxiang , Kuo Fengyi , Zhang Yiran , Wang Duan , He Li , Yang Ying , Wang Hepeng , Chen Yanni
Published: June 2022

Abnormal alterations in enzymes functioned in sialic acid modifications may be associated with ASD. In order to study the differences in peripheral blood sialidase (neuraminidase 1; NEU1) mRNA expression between autism spectrum disorder (ASD) children and healthy control, and to examine the correlation between NEU1 mRNA expression and the main behavioral phenotypes in children with ASD, we performed RT-qPCR to measure NEU1 mRNA expression in peripheral blood of 42 children with ASD and 42 healthy controls. In addition, we used the Autism Diagnostic Observation Schedule, Second Edition (ADOS-2) to measure and evaluate the behavioral phenotypes of children with ASD. Our results showed that NEU1 mRNA in the ASD group was significantly higher than in the control group (P < 0.0001). In addition, the ADOS-2 diagnostic scores of 42 children with ASD were correlated with their NEU1 mRNA expression results (R = 0.344, P = 0.0257). Moreover, in general, NEU1 mRNA expression was also positively correlated with the Social Affect (SA) of ADOS-2 (R = 0.3598, P = 0.0193) but not with the Restricted and Repetitive Behavior (RRB) (R = 0.15, P = 0.3432). Our results indicated that sialidase NEU1 mRNA was significantly increased in children with ASD, and its expression was correlated with the SA of children with ASD, which suggested that sialidase NEU1 may affect the SA of ASD. Our data highlighted the potential of NEU1 expression change may play an important role in ASD disease and lay the foundation for further studies on the relationship between NEU1 and ASD.
Efficacy and Tolerability of Cranial Electrotherapy Stimulation in the Treatment of Anxiety: A Systemic Review and Meta-Analysis
Authors: Ching Pao-Yuan , Hsu Tien-Wei , Chen Guan-Wei , Pan Chih-Chuan , Chu Che-Sheng , Chou Po-Han
Published: June 2022

Objective

We aimed to investigate the efficacy and tolerability of cranial electrotherapy stimulation (CES) for patients with anxiety symptoms.

Method

We searched the Pubmed, Cochrane Central Register of Controlled Trials (CENTRAL), Embase and Medline for randomized control trials (RCTs) from the time of inception until November 15, 2021, following Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines. Data were pooled using a random-effects model. The primary outcomes were the mean change scores for anxiety symptoms. The secondary outcomes were the mean change scores for depressive symptoms.

Results

Eleven RCTs were eligible (n = 794, mean age: 41.4, mean population of female: 64.8%). CES significantly reduced the anxiety symptoms compared to the control group [k = 11, n = 692, Hedge's g = −0.625, 95% confidence intervals (CIs) = −0.952 to −0.298, P < 0.001] with moderate effect size. The subgroup analysis showed that CES reduced both primary and secondary anxiety (primary anxiety, k =3, n = 288, Hedges' g = −1.218, 95% CIs = −1.418 to −0.968, P = 0.007; secondary anxiety, k = 8, n = 504, Hedges' g = −0.334, 95% CIs = −0.570 to −0.098, P = 0.006). After performing between group analysis, we found CES has significant better efficacy for patients with primary anxiety than those with secondary anxiety (P < 0.001). For secondary outcome, CES significantly reduced depressive symptoms in patients with anxiety disorders (k = 8, n = 552, Hedges' g = −0.648, 95% CIs = −1.062 to −0.234, P = 0.002). No severe side effects were reported and the most commonly reported adverse events were ear discomfort and ear pain.

Conclusion

We found CES is effective in reducing anxiety symptoms with moderate effect size in patients with both primary and secondary anxiety. Furthermore, CES was well-tolerated and acceptable.

Systematic Review Registration: PROSPERO, https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021267916.
Relationship Between Post-traumatic Stress Symptoms and Anticipatory Grief in Family Caregivers of Patients With Advanced Lung Cancer: The Mediation Role of Illness Uncertainty
Authors: Sun Di , Mao Zhihui , Zhang Xu , Li Jiaojiao , Zhang Lijuan
Published: June 2022

Objective

To explore the interrelationship between post-traumatic stress symptoms (PTSS), illness uncertainty (IU), and anticipatory grief (AG).

Methods

Structural equation modeling with bootstrapping estimation was conducted using data from a convenience sample of 254 family caregivers of patients with advanced lung cancer in China. Participants were recruited from a public cancer hospital in Shenyang, China. The family caregivers completed the Impact of Events Scale-Revised, Uncertainty in Illness Scale Family Caregiver Version, and Anticipatory Grief Scale.

Results

The measurement model has good reliability and validity, and the final model fit the data well. PTSS positively influenced AG (direct effect estimate = 0.391, p = 0.002). Moreover, IU was found to mediate the relationship between PTSS and AG (Indirect effects estimate = 0.168, p = 0.005). The mediating effect of IU accounted for up to 30.1% of the total effect.

Conclusion

IU mediated the relationship between PTSS and AG. Healthcare professionals should continuously assess PTSS, IU and AG levels in FCs and provide effective intervention options for mitigation.