bluuebunny/crossref_metadata_2025 huggingface dataset:
Theosis and Religion
Authors: Russell Norman
Published: March 2024

Theosis, originally a Greek term for Christian divinisation or deification, has become a vogue word in modern theology. Although recent publications have explored its meaning in a selection of different contexts, this is the first book to offer a coherent narrative of how the concept of theosis developed in both its Eastern and Western versions. Norman Russell shows how the role of Dionysius the Areopagite was pivotal, not only in Byzantium but also in the late mediaeval West, where it strengthened the turn towards an individualistic interiority. Russell also relates theosis to changing concepts of religion in the modern age. He investigates the Russian version of theosis, introduced in the West by Russian members the Paris School after the 1917 Revolution. Since then, theosis has undergone additional development through the addition of esoteric elements which have since passed into the mainstream of all theological traditions and even into popular spirituality.
Three Faces of Sun Tzu
Authors: Boorman Scott
Published: March 2024

Sun Tzu's Art of War is widely regarded as the most influential military & strategic classic of all time. Through 'reverse engineering' of the text structured around 14 Sun Tzu 'themes,' this rigorous analysis furnishes a thorough picture of what the text actually says, drawing on Chinese-language analyses, historical, philological, & archaeological sources, traditional commentaries, computational ideas, and strategic & logistics perspectives. Building on this anchoring, the book provides a unique roadmap of Sun Tzu's military and intelligence insights and their applications to strategic competitions in many times and places worldwide, from Warring States China to contemporary US/China strategic competition and other 21st century competitions involving cyber warfare, computing, other hi-tech conflict, espionage, and more. Simultaneously, the analysis offers a window into Sun Tzu's limitations and blind spots relevant to managing 21st century strategic competitions with Sun-Tzu-inspired adversaries or rivals.
Cognitive and Social Neuroscience of Aging
Authors: Gutchess Angela
Published: March 2024

Cognitive and Social Neuroscience of Aging is an introduction to how aging affects the brain, intended for audiences with some knowledge of psychology, aging, or neuroscience. The book includes figures illustrating brain regions so that extensive familiarity with neuroanatomy is not a pre-requisite. The depth of coverage also makes this book appropriate for those with considerable knowledge about aging. This book adopts an integrative perspective, including topics such as memory, cognition, cognitive training, emotion, and social processes. Topics include consideration of individual differences and the impact of disorders (e.g. Alzheimer's disease) on brain function with age. Although many declines occur with age, cognitive neuroscience research reveals plasticity and adaptation in the brain as a function of normal aging. This book is written with this perspective in mind, emphasizing the ways in which neuroscience methods have enriched and changed thinking about aging.
Chinese Perspectives on the Rule of Law in International Economic Law
Authors: Xiao Jun
Published: March 2024

In the currently volatile global economic environment, China’s perspectives on the rule of law in international economic law are of significance. In principle, China shares developing countries’ attitudes towards international economic law. China also seeks to play a constructive role in the development of international economic law, endorsing a flexible and pragmatic approach. China has concluded international trade and investment treaties designed to constrain its policy space and is an active supporter of the World Trade Organization’s dispute settlement and investor-state dispute settlement mechanisms, with a good record of compliance. Recent trade tensions demonstrate challenges for the international rule of law but also emphasise the role of law in overcoming political challenges.
Between automated memory and history: blocking ‘sensitive locations’ from Apple Memories
Authors: Vilvang Chrys
Published: March 2024

In April 2022, journalists at the tech website 9to5Mac discovered that photographs taken at sites related to the Holocaust would no longer appear in the Memories feature of Apple's Photos app. This article examines how news of this decision was received by the public through analysis of the comment section that followed the original 9to5Mac post. The perspectives on display in this public forum provide insight into the evolving public perception of automated memory technologies and the potential consequences of their use. Through this analysis, several interrelated areas of public concern emerge. These include the boundaries of platform intervention for governing access to content, the subjective qualities of personal photographs, and the metrics upon which algorithmic memory systems operate. Though opinions vary, this comment section captures an illustrative range of sentiment towards Apple Memories and this intervention into the memories of its users. This range demonstrates a degree of scepticism, alarm, and dissatisfaction rising among users who are increasingly aware of how algorithms are influencing their memories.
Two new species of the genus Lecidella (Lecanoraceae, Ascomycota) from maritime Antarctica, southern South America and North America
Authors: Ruprecht Ulrike , Avci Feyza Nur , Candan Mehmet , Halıcı Mehmet Gökhan
Published: March 2024

Two new species of the genus Lecidella, one with a North American-maritime Antarctic distribution and one with a so far exclusively southern South American-maritime Antarctic distribution, are described using molecular and morphological tools. Lecidella ayazii is a species growing on soil and also on mosses and has so far been found on the Antarctic Peninsula, as well as in the alpine areas of the La Sal Mountains, Utah, USA and in the Kivalliq Region (Nunavut) in the north of Canada, whereas L. drakensis occurs mainly on siliceous rocks, rarely on mosses, and has been recorded on both sides of the Drake Passage in southern Patagonia and the Antarctic Peninsula. Phylogenetic analysis of the nrITS sequence data shows that both species belong in the L. elaeochroma clade, each forming a highly supported and distinct group. Furthermore, they also differ in morphological and chemical characters from the species described so far in this clade. In addition, five further accessions were recorded from the maritime Antarctic, which were placed in the cosmopolitan and heterogeneous L. stigmatea clade, of which one could be assigned to the bipolar species L. siplei.
Neurocognitive correlates of metabolic dysregulation in individuals with mood disorders: a systematic review and meta-analysis
Authors: Maksyutynska Kateryna , Stogios Nicolette , Prasad Femin , Gill Jashan , Hamza Zaineb , De Riddhita , Smith Emily , Horta Angelina , Goldstein Benjamin I. , Korczak Daphne , Graff-Guerrero Ariel , Hahn Margaret K. , Agarwal Sri Mahavir
Published: March 2024

Individuals with mood disorders are predisposed to metabolic dysfunction, while those with metabolic dysregulation such as diabetes and obesity experience more severe depressive symptoms. Both metabolic dysfunction and mood disorders are independently associated with cognitive deficits. Therefore, given their close association, this study aimed to explore the association between metabolic dysfunction in individuals with mood disorders in relation to cognitive outcomes. A comprehensive search comprised of these three domains was carried out; a random-effects meta-analysis pooling mean cognitive outcomes was conducted (PROSPERO ID: CRD42022295765). Sixty-three studies were included in this review; 26 were synthesized in a quantitative meta-analysis. Comorbid metabolic dysregulation was associated with significantly lower global cognition among individuals with mood disorders. These trends were significant within each mood disorder subgroup, including major depressive disorder, bipolar disorder, and self-report depression/depressive symptoms. Type 2 diabetes was associated with the lowest cognitive performance in individuals with mood disorders, followed by peripheral insulin resistance, body mass index ⩾25 kg/m2, and metabolic syndrome. Significant reduction in scores was also observed among individual cognitive domains (in descending order) of working memory, attention, executive function, processing speed, verbal memory, and visual memory. These findings demonstrate the detrimental effects of comorbid metabolic dysfunction in individuals with mood disorders. Further research is required to understand the underlying mechanisms connecting mood disorders, metabolism, and cognition.
Impartial Spectators and Legislators: Adam Smith and Religion
Authors: Lentz Rachel , Tuckness Alex
Published: March 2024

The eighteenth century is often thought of as an important era for the secularization of British philosophy. One dimension of this secularization involves philosophers developing theories that, while not explicitly rejecting God, are less reliant on claims about God for their intelligibility and plausibility. A comparison of Adam Smith with his teacher, Francis Hutcheson, reveals an underappreciated facet of this process. Hutcheson portrayed God as an impartial legislator and used this metaphor to clarify contested moral rules in a way that would make little sense if God did not exist. In contrast, Smith's impartial spectator does not require belief in a God who is the author of the moral law in order to determine the correct interpretation of contested moral principles. By pursuing this alternative conception of impartiality, Smith developed a theory that was, in this respect, less reliant on religious premises than Hutcheson's had been.
Actuarial applications of natural language processing using transformers: Case studies for using text features in an actuarial context
Authors: Troxler Andreas , Schelldorfer Jürg
Published: March 2024

This paper demonstrates workflows to incorporate text data into actuarial classification and regression tasks. The main focus is on methods employing transformer-based models. A dataset of car accident descriptions with an average length of 400 words, available in English and German, and a dataset with short property insurance claims descriptions, are used to demonstrate these techniques. The case studies tackle challenges related to a multilingual setting and long input sequences. They also show ways to interpret model output and to assess and improve model performance, by fine-tuning the models to the domain of application or to a specific prediction task. Finally, the paper provides practical approaches to handle classification tasks in situations with no or only few labelled data. The results achieved by using the language-understanding skills of off-the-shelf natural language processing (NLP) models with only minimal pre-processing and fine-tuning clearly demonstrate the power of transfer learning for practical applications.
The Energetic Oxygen Ion Beams in the Martian Magnetotail Current Sheets: Hints From the Comparisons Between Two Types of Current Sheets
Authors: Zhang Chi , Rong Zhaojin , Li Xinzhou , Fränz Markus , Nilsson Hans , Jarvinen Riku , Persson Moa , Futaana Yoshifumi , Dong Chuanfei , Yamauchi Masatoshi , Gao Jiawei , Zhou Yijia , Wang Lei , Shi Zhen , Wei Yong , He Fei , Holmström Mats , Barabash Stas
Published: March 2024

Using data from the Mars Atmosphere and Volatile EvolutioN (MAVEN) mission, we explore the plasma properties of Martian magnetotail current sheets (CS), to further understand the solar wind interaction with Mars and ion escape. There are some CS exhibit energetic oxygen ions that show narrow beam structures in the energy spectrum, which primarily occurs in the hemisphere where the solar wind electric field (Esw) is directed away from the planet. On average, these CS have a higher escaping flux than that of the CS without energetic oxygen ion beams, suggesting different roles in ion escape. The CS with energetic oxygen ion beams exhibits different proton and electron properties to the CS without energetic oxygen ion beams, indicating their different origins. Our analysis suggests that the CS with energetic oxygen ion beams may result from the interaction between the penetrated solar wind and localized oxygen ion plumes.
New Magnetotelluric Data Reveal Deep Fault Boundaries and Contrasting Late Cenozoic Fault Kinematics Between the Qilian Shan Thrust Wedge and Beishan‐Alxa Block, Western China
Authors: Yang Haibo , Sun Xiangyu , Zhan Yan , Yang Xiaoping , Cunningham Dickson , Zhao Lingqiang , Zuo Yuqi
Published: March 2024

The structural connectivity and kinematic relationship between the Altyn Tagh sinistral strike‐slip fault (ATF) and Qilian Shan fold‐and‐thrust belt along the north Tibetan margin east of 96°E is an important question for tectonicists interested in the evolving active deformation field of Central Asia and associated earthquake hazards of China's Hexi Corridor region. New results from a detailed 130‐km‐long N‐S magnetotelluric (MT) survey from the Qilian Shan to Beishan elucidates the locations and down‐dip orientations of major faults. Importantly, the results indicate that the Heishan‐Jinta’Nanshan fault system roots steeply into the lower crust, is unconnected to the Qilian Shan thrust wedge, and has reactivated the margin of the North China Craton and an older, regional ductile shear belt. The structurally linked ATF‐Heishan‐Jinta’Nanshan system defines a fundamental kinematic boundary in central Asia between the NE directed Qilian Shan thrust belt to the south and the eastwardly extruding Beishan‐Alxa Block to the north.
Ocean Bottom Distributed Acoustic Sensing for Oceanic Seismicity Detection and Seismic Ocean Thermometry
Authors: Shen Zhichao , Wu Wenbo
Published: March 2024

A T‐wave is a seismo‐acoustic wave that can travel a long distance in the ocean with little attenuation, making it valuable for monitoring remote tectonic activity and changes in ocean temperature using seismic ocean thermometry (SOT). However, current high‐quality T‐wave stations are sparsely distributed, limiting the detectability of oceanic seismicity and the spatial resolution of global SOT. The use of ocean bottom distributed acoustic sensing (OBDAS), through the conversion of telecommunication cables into dense seismic arrays, is a cost‐effective and scalable means to complement existing seismic stations. Here, we systematically investigate the performance of OBDAS for oceanic seismicity detection and SOT using a 4‐day Ocean Observatories Initiative community experiment offshore Oregon. We first present T‐wave observations from distant and regional earthquakes and develop a curvelet denoising scheme to enhance T‐wave signals on OBDAS. After denoising, we show that OBDAS can detect and locate more and smaller T‐wave events than regional OBS network. During the 4‐day experiment, we detect 92 oceanic earthquakes, most of which are missing from existing catalogs. Leveraging the sensor density and cable directionality, we demonstrate the feasibility of source azimuth estimation for regional Blanco earthquakes. We also evaluate the SOT performance of OBDAS using pseudo‐repeating earthquake T‐waves. Our results show that OBDAS can utilize repeating earthquakes as small as M3.5 for SOT, outperforming ocean bottom seismometers. However, ocean ambient natural and instrumental noise strongly affects the performance of OBDAS for oceanic seismicity detection and SOT, requiring further investigation.
Targeting carnitine palmitoyl transferase 1A (CPT1A) induces ferroptosis and synergizes with immunotherapy in lung cancer
Authors: Ma Lei , Chen Chong , Zhao Chunxing , Li Tong , Ma Lingyu , Jiang Jiayu , Duan Zhaojun , Si Qin , Chuang Tsung-Hsien , Xiang Rong , Luo Yunping
Published: March 2024

Despite the successful application of immune checkpoint therapy, no response or recurrence is typical in lung cancer. Cancer stem cells (CSCs) have been identified as a crucial player in immunotherapy-related resistance. Ferroptosis, a form of cell death driven by iron-dependent lipid peroxidation, is highly regulated by cellular metabolism remolding and has been shown to have synergistic effects when combined with immunotherapy. Metabolic adaption of CSCs drives tumor resistance, yet the mechanisms of their ferroptosis defense in tumor immune evasion remain elusive. Here, through metabolomics, transcriptomics, a lung epithelial-specific Cpt1a-knockout mouse model, and clinical analysis, we demonstrate that CPT1A, a key rate-limiting enzyme of fatty acid oxidation, acts with L-carnitine, derived from tumor-associated macrophages to drive ferroptosis-resistance and CD8+ T cells inactivation in lung cancer. Mechanistically, CPT1A restrains ubiquitination and degradation of c-Myc, while c-Myc transcriptionally activates CPT1A expression. The CPT1A/c-Myc positive feedback loop further enhances the cellular antioxidant capacity by activating the NRF2/GPX4 system and reduces the amount of phospholipid polyunsaturated fatty acids through ACSL4 downregulating, thereby suppressing ferroptosis in CSCs. Significantly, targeting CPT1A enhances immune checkpoint blockade-induced anti-tumor immunity and tumoral ferroptosis in tumor-bearing mice. The results illustrate the potential of a mechanism-guided therapeutic strategy by targeting a metabolic vulnerability in the ferroptosis of CSCs to improve the efficacy of lung cancer immunotherapy.
Reducing and controlling metabolic active tumor volume prior to CAR T-cell infusion can improve survival outcomes in patients with large B-cell lymphoma
Authors: Keijzer Kylie , de Boer Janneke W. , van Doesum Jaap A. , Noordzij Walter , Huls Gerwin A. , van Dijk Lisanne V. , van Meerten Tom , Niezink Anne G. H.
Published: March 2024

Bridging therapy before CD19-directed chimeric antigen receptor (CAR) T-cell infusion is frequently applied in patients with relapsed or refractory Large B-cell lymphoma (r/r LBCL). This study aimed to assess the influence of quantified MATV and MATV-dynamics, between pre-apheresis (baseline) and pre-lymphodepleting chemotherapy (pre-LD) MATV, on CAR T-cell outcomes and toxicities in patients with r/r LBCL. MATVs were calculated semi-automatically at baseline (n = 74) and pre-LD (n = 68) in patients with r/r LBCL who received axicabtagene ciloleucel. At baseline, patients with a low MATV (< 190 cc) had a better time to progression (TTP) and overall survival (OS) compared to high MATV patients (p < 0.001). High MATV patients who remained stable or reduced upon bridging therapy showed a significant improvement in TTP (p = 0.041) and OS (p = 0.015), compared to patients with a high pre-LD MATV (> 480 cc). Furthermore, high MATV baseline was associated with severe cytokine release syndrome (CRS, p = 0.001). In conclusion, patients with low baseline MATV had the best TTP/OS and effective reduction or controlling MATV during bridging improved survival outcomes in patients with a high baseline MATV, providing rationale for the use of more aggressive bridging regimens.
The effects of metabolism on the immune microenvironment in colorectal cancer
Authors: Chen Xingzhao , Ma Zhiyuan , Yi Zhiqiang , Wu Enqin , Shang Zhengye , Tuo Biguang , Li Taolang , Liu Xuemei
Published: March 2024

Colorectal cancer (CRC) is a malignancy that is widely prevalent worldwide. Due to its unsatisfactory treatment outcome and extremely poor prognosis, many studies on the molecular mechanisms and pathological mechanisms of CRC have been published in recent years. The tumor microenvironment (TME) is an extremely important feature of tumorigenesis and one of the hallmarks of tumor development. Metabolic reprogramming is currently a hot topic in tumor research, and studies on this topic have provided important insights into CRC development. In particular, metabolic reprogramming in cancer causes changes in the composition of energy and nutrients in the TME. Furthermore, it can alter the complex crosstalk between immune cells and associated immune factors, such as associated macrophages and T cells, which play important immune roles in the TME, in turn affecting the immune escape of tumors by altering immune surveillance. In this review, we summarize several metabolism-related processes affecting the immune microenvironment of CRC tumors. Our results showed that the immune microenvironment is regulated by metabolic reprogramming and influences the development of CRC.
Single cell transcriptome analysis of the THY-Tau22 mouse model of Alzheimer’s disease reveals sex-dependent dysregulations
Authors: Ali Muhammad , Garcia Pierre , Lunkes Laetitia P. , Sciortino Alessia , Thomas Melanie , Heurtaux Tony , Grzyb Kamil , Halder Rashi , Coowar Djalil , Skupin Alex , Buée Luc , Blum David , Buttini Manuel , Glaab Enrico
Published: March 2024

Alzheimer’s disease (AD) progression and pathology show pronounced sex differences, but the factors driving these remain poorly understood. To gain insights into early AD-associated molecular changes and their sex dependency for tau pathology in the cortex, we performed single-cell RNA-seq in the THY-Tau22 AD mouse model. By examining cell type-specific and cell type-agnostic AD-related gene activity changes and their sex-dimorphism for individual genes, pathways and cellular sub-networks, we identified both statistically significant alterations and interpreted the upstream mechanisms controlling them. Our results confirm several significant sex-dependent alterations in gene activity in the THY-Tau22 model mice compared to controls, with more pronounced alterations in females. Both changes shared across multiple cell types and cell type-specific changes were observed. The differential genes showed significant over-representation of known AD-relevant processes, such as pathways associated with neuronal differentiation, programmed cell death and inflammatory responses. Regulatory network analysis of these genes revealed upstream regulators that modulate many of the downstream targets with sex-dependent changes. Most key regulators have been previously implicated in AD, such asEgr1,Klf4,Chchd2, complement system genes, and myelin-associated glycoproteins. Comparing with similar data from the Tg2576 AD mouse model and human AD patients, we identified multiple genes with consistent, cell type-specific and sex-dependent alterations across all three datasets. These shared changes were particularly evident in the expression of myelin-associated genes such asMbpandPlp1in oligodendrocytes. In summary, we observed significant cell type-specific transcriptomic changes in the THY-Tau22 mouse model, with a strong over-representation of known AD-associated genes and processes. These include both sex-neutral and sex-specific patterns, characterized by consistent shifts in upstream master regulators and downstream target genes. Collectively, these findings provide insights into mechanisms influencing sex-specific susceptibility to AD and reveal key regulatory proteins that could be targeted for developing treatments addressing sex-dependent AD pathology.
Social buffering in rats reduces fear by oxytocin triggering sustained changes in central amygdala neuronal activity
Authors: Hegoburu Chloe , Tang Yan , Niu Ruifang , Ghosh Supriya , Triana Del Rio Rodrigo , de Araujo Salgado Isabel , Abatis Marios , Alexandre Mota Caseiro David , van den Burg Erwin H. , Grundschober Christophe , Stoop Ron
Published: March 2024

The presence of a companion can reduce fear, but the neural mechanisms underlying this social buffering of fear are incompletely known. We studied social buffering of fear in male and female, and its encoding in the amygdala of male, auditory fear-conditioned rats. Pharmacological, opto,- and/or chemogenetic interventions showed that oxytocin signaling from hypothalamus-to-central amygdala projections underlied fear reduction acutely with a companion and social buffering retention 24 h later without a companion. Single-unit recordings with optetrodes in the central amygdala revealed fear-encoding neurons (showing increased conditioned stimulus-responses after fear conditioning) inhibited by social buffering and blue light-stimulated oxytocinergic hypothalamic projections. Other central amygdala neurons showed baseline activity enhanced by blue light and companion exposure, with increased conditioned stimulus responses that persisted without the companion. Social buffering of fear thus switches the conditioned stimulus from encoding “fear” to “safety” by oxytocin-mediated recruitment of a distinct group of central amygdala “buffer neurons”.
The North Equatorial Current and rapid intensification of super typhoons
Authors: Kang Sok Kuh , Kim Sung-Hun , Lin I.-I. , Park Young-Hyang , Choi Yumi , Ginis Isaac , Cione Joseph , Shin Ji Yun , Kim Eun Jin , Kim Kyeong Ok , Kang Hyoun Woo , Park Jae-Hyoung , Bidlot Jean-Raymond , Ward Brian
Published: March 2024

Super Typhoon Mangkhut, which traversed the North Equatorial Current (NEC; 8–17 °N) in the western North Pacific in 2018, was the most intense Category-5 tropical cyclone (TC) with the longest duration in history—3.5 days. Here we show that the combination of two factors—high ocean heat content (OHC) and increased stratification — makes the NEC region the most favored area for a rapid intensification (RI) of super typhoons, instead of the Eddy Rich Zone (17–25 °N), which was considered the most relevant for RI occurrence. The high OHC results from a northward deepening thermocline in geostrophic balance with the westward-flowing NEC. The stratification is derived from precipitation associated with the Inter-Tropical Convergence Zone in the summer peak typhoon season. These factors, which are increasingly significant over the past four decades, impede the TC-induced sea surface cooling, thus enhancing RI of TCs and simultaneously maintaining super typhoons over the NEC region.
Kdm1a safeguards the topological boundaries of PRC2-repressed genes and prevents aging-related euchromatinization in neurons
Authors: del Blanco Beatriz , Niñerola Sergio , Martín-González Ana M. , Paraíso-Luna Juan , Kim Minji , Muñoz-Viana Rafael , Racovac Carina , Sanchez-Mut Jose V. , Ruan Yijun , Barco Ángel
Published: March 2024

Kdm1a is a histone demethylase linked to intellectual disability with essential roles during gastrulation and the terminal differentiation of specialized cell types, including neurons, that remains highly expressed in the adult brain. To explore Kdm1a’s function in adult neurons, we develop inducible and forebrain-restricted Kdm1a knockouts. By applying multi-omic transcriptome, epigenome and chromatin conformation data, combined with super-resolution microscopy, we find that Kdm1a elimination causes the neuronal activation of nonneuronal genes that are silenced by the polycomb repressor complex and interspersed with active genes. Functional assays demonstrate that the N-terminus of Kdm1a contains an intrinsically disordered region that is essential to segregate Kdm1a-repressed genes from the neighboring active chromatin environment. Finally, we show that the segregation of Kdm1a-target genes is weakened in neurons during natural aging, underscoring the role of Kdm1a safeguarding neuronal genome organization and gene silencing throughout life.
Cas9-assisted biological containment of a genetically engineered human commensal bacterium and genetic elements
Authors: Hayashi Naoki , Lai Yong , Fuerte-Stone Jay , Mimee Mark , Lu Timothy K.
Published: March 2024

Sophisticated gene circuits built by synthetic biology can enable bacteria to sense their environment and respond predictably. Engineered biosensing bacteria outfitted with such circuits can potentially probe the human gut microbiome to prevent, diagnose, or treat disease. To provide robust biocontainment for engineered bacteria, we devised a Cas9-assisted auxotrophic biocontainment system combining thymidine auxotrophy, an Engineered Riboregulator (ER) for controlled gene expression, and a CRISPR Device (CD). The CD prevents the engineered bacteria from acquiring thyA via horizontal gene transfer, which would disrupt the biocontainment system, and inhibits the spread of genetic elements by killing bacteria harboring the gene cassette. This system tunably controlled gene expression in the human gut commensal bacterium Bacteroides thetaiotaomicron, prevented escape from thymidine auxotrophy, and blocked transgene dissemination. These capabilities were validated in vitro and in vivo. This biocontainment system exemplifies a powerful strategy for bringing genetically engineered microorganisms safely into biomedicine.
Circulation of hydraulically ponded turbidity currents and the filling of continental slope minibasins
Authors: Reece J. Kevin , Dorrell Robert M. , Straub Kyle M.
Published: March 2024

Natural depressions on continental margins termed minibasins trap turbidity currents, a class of sediment-laden seafloor density driven flow. These currents are the primary downslope vectors for clastic sediment, particulate organic carbon, and microplastics. Here, we establish a method that facilitates long-distance self-suspension of dilute sediment-laden flows, enabling study of turbidity currents with appropriately scaled natural topography. We show that flow dynamics in three-dimensional minibasins are dominated by circulation cell structures. While fluid rotation is mainly along a horizontal plane, inwards spiraling flow results in strong upwelling jets that reduce the ability of minibasins to trap particulate organic carbon, microplastics, and fine-grained clastic sediment. Circulation cells are the prime mechanism for distributing particulates in minibasins and set the geometry of deposits, which are often intricate and below the resolution of geophysical surveys. Fluid and sediment are delivered to circulation cells by turbidity currents that runup the distal wall of minibasins. The magnitude of runup increases with the discharge rate of currents entering minibasins, which influences the amount of sediment that is either trapped in minibasins or spills to downslope environs and determines the height that deposits onlap against minibasin walls.
Dimerization-dependent serine protease activity of FAM111A prevents replication fork stalling at topoisomerase 1 cleavage complexes
Authors: Palani Sowmiya , Machida Yuka , Alvey Julia R. , Mishra Vandana , Welter Allison L. , Cui Gaofeng , Bragantini Benoît , Botuyan Maria Victoria , Cong Anh T. Q. , Mer Georges , Schellenberg Matthew J. , Machida Yuichi J.
Published: March 2024

FAM111A, a serine protease, plays roles in DNA replication and antiviral defense. Missense mutations in the catalytic domain cause hyper-autocleavage and are associated with genetic disorders with developmental defects. Despite the enzyme’s biological significance, the molecular architecture of the FAM111A serine protease domain (SPD) is unknown. Here, we show that FAM111A is a dimerization-dependent protease containing a narrow, recessed active site that cleaves substrates with a chymotrypsin-like specificity. X-ray crystal structures and mutagenesis studies reveal that FAM111A dimerizes via the N-terminal helix within the SPD. This dimerization induces an activation cascade from the dimerization sensor loop to the oxyanion hole through disorder-to-order transitions. Dimerization is essential for proteolytic activity in vitro and for facilitating DNA replication at DNA-protein crosslink obstacles in cells, while it is dispensable for autocleavage. These findings underscore the role of dimerization in FAM111A’s function and highlight the distinction in its dimerization dependency between substrate cleavage and autocleavage.
Tri-system integration in metal-oxide nanocomposites via in-situ solution-processed method for ultrathin flexible transparent electrodes
Authors: Kim John Jinwook , Shuji Kojima , Zheng Jiawei , He Xinjun , Sajjad Ahmad , Zhang Hong , Su Haibin , Choy Wallace C. H.
Published: March 2024

For stable operation of ultrathin flexible transparent electrodes (uFTEs), it is critical to implement effective risk management during concurrent multi-loading operation of electrical bias and mechanical folding cycles in high-humidity environments. Despite extensive efforts in preparing solution-processed uFTEs with cost-effective and high-throughput means, achieving in-situ nano-adhesion in heterogeneous metal-oxide nanocomposites remains challenging. In this work, we observed by serendipity liquid-like behaviour of transparent metal-oxide-semiconductor zinc oxide nanoparticles (ZnONPs) onto silver nanowires (AgNWs) developed by in-situ solution processed method (iSPM). This enabled us to address the long-standing issue of vulnerability in the nanocomposite caused by the interface of dissimilar materials between AgNWs and ZnONPs, resulting in a remarkably improved multi-loading operation. Importantly, substrate-integrated uFTEs constituted of the metal-oxide nanocomposite electrode semi-embedded in the polymer matrix of greatly thin <0.5 μm thickness is successfully demonstrated with the smooth surface topography, promoted by the tri-system integration including (i) AgNW-AgNW, (ii) ZnONP-ZnONP, and (iii) AgNW-ZnONP systems. Our finding unveils the complex interfacial dynamics associated with the heterogeneous interface system between AgNWs and ZnONPs and holds great promise in understanding the in-situ nano-adhesion process and increasing the design flexibility of next generation solution-processed uFTEs.
Room temperature chirality switching and detection in a helimagnetic MnAu2 thin film
Authors: Masuda Hidetoshi , Seki Takeshi , Ohe Jun-ichiro , Nii Yoichi , Masuda Hiroto , Takanashi Koki , Onose Yoshinori
Published: March 2024

Helimagnetic structures, in which the magnetic moments are spirally ordered, host an internal degree of freedom called chirality corresponding to the handedness of the helix. The chirality seems quite robust against disturbances and is therefore promising for next-generation magnetic memory. While the chirality control was recently achieved by the magnetic field sweep with the application of an electric current at low temperature in a conducting helimagnet, problems such as low working temperature and cumbersome control and detection methods have to be solved in practical applications. Here we show chirality switching by electric current pulses at room temperature in a thin-film MnAu2 helimagnetic conductor. Moreover, we have succeeded in detecting the chirality at zero magnetic fields by means of simple transverse resistance measurement utilizing the spin Berry phase in a bilayer device composed of MnAu2 and a spin Hall material Pt. These results may pave the way to helimagnet-based spintronics.
Canonical and non-canonical integrin-based adhesions dynamically interconvert
Authors: Lukas Fabian , Matthaeus Claudia , López-Hernández Tania , Lahmann Ines , Schultz Nicole , Lehmann Martin , Puchkov Dmytro , Pielage Jan , Haucke Volker , Maritzen Tanja
Published: March 2024

Adhesions are critical for anchoring cells in their environment, as signaling platforms and for cell migration. In line with these diverse functions different types of cell-matrix adhesions have been described. Best-studied are the canonical integrin-based focal adhesions. In addition, non-canonical integrin adhesions lacking focal adhesion proteins have been discovered. These include reticular adhesions also known as clathrin plaques or flat clathrin lattices, that are enriched in clathrin and other endocytic proteins, as well as extensive adhesion networks and retraction fibers. How these different adhesion types that share a common integrin backbone are related and whether they can interconvert is unknown. Here, we identify the protein stonin1 as a marker for non-canonical αVβ5 integrin-based adhesions and demonstrate by live cell imaging that canonical and non-canonical adhesions can reciprocally interconvert by the selective exchange of components on a stable αVβ5 integrin scaffold. Hence, non-canonical adhesions can serve as points of origin for the generation of canonical focal adhesions.
Neuromorphic antennal sensory system
Authors: Jiang Chengpeng , Xu Honghuan , Yang Lu , Liu Jiaqi , Li Yue , Takei Kuniharu , Xu Wentao
Published: March 2024

Insect antennae facilitate the nuanced detection of vibrations and deflections, and the non-contact perception of magnetic or chemical stimuli, capabilities not found in mammalian skin. Here, we report a neuromorphic antennal sensory system that emulates the structural, functional, and neuronal characteristics of ant antennae. Our system comprises electronic antennae sensor with three-dimensional flexible structures that detects tactile and magnetic stimuli. The integration of artificial synaptic devices adsorbed with solution-processable MoS2 nanoflakes enables synaptic processing of sensory information. By emulating the architecture of receptor-neuron pathway, our system realizes hardware-level, spatiotemporal perception of tactile contact, surface pattern, and magnetic field (detection limits: 1.3 mN, 50 μm, 9.4 mT). Vibrotactile-perception tasks involving profile and texture classifications were accomplished with high accuracy (> 90%), surpassing human performance in “blind” tactile explorations. Magneto-perception tasks including magnetic navigation and touchless interaction were successfully completed. Our work represents a milestone for neuromorphic sensory systems and biomimetic perceptual intelligence.
A root cap-localized NAC transcription factor controls root halotropic response to salt stress in Arabidopsis
Authors: Zheng Lulu , Hu Yongfeng , Yang Tianzhao , Wang Zhen , Wang Daoyuan , Jia Letian , Xie Yuanming , Luo Long , Qi Weicong , Lv Yuanda , Beeckman Tom , Xuan Wei , Han Yi
Published: March 2024

Plants are capable of altering root growth direction to curtail exposure to a saline environment (termed halotropism). The root cap that surrounds root tip meristematic stem cells plays crucial roles in perceiving and responding to environmental stimuli. However, how the root cap mediates root halotropism remains undetermined. Here, we identified a root cap-localized NAC transcription factor, SOMBRERO (SMB), that is required for root halotropism. Its effect on root halotropism is attributable to the establishment of asymmetric auxin distribution in the lateral root cap (LRC) rather than to the alteration of cellular sodium equilibrium or amyloplast statoliths. Furthermore, SMB is essential for basal expression of the auxin influx carrier gene AUX1 in LRC and for auxin redistribution in a spatiotemporally-regulated manner, thereby leading to directional bending of roots away from higher salinity. Our findings uncover an SMB-AUX1-auxin module linking the role of the root cap to the activation of root halotropism.
Interrogating site dependent kinetics over SiO2-supported Pt nanoparticles
Authors: Kim Taek-Seung , O’Connor Christopher R. , Reece Christian
Published: March 2024

A detailed knowledge of reaction kinetics is key to the development of new more efficient heterogeneous catalytic processes. However, the ability to resolve site dependent kinetics has been largely limited to surface science experiments on model systems. Herein, we can bypass the pressure, materials, and temperature gaps, resolving and quantifying two distinct pathways for CO oxidation over SiO2-supported 2 nm Pt nanoparticles using transient pressure pulse experiments. We find that the pathway distribution directly correlates with the distribution of well-coordinated (e.g., terrace) and under-coordinated (e.g., edge, vertex) CO adsorption sites on the 2 nm Pt nanoparticles as measured by in situ DRIFTS. We conclude that well-coordinated sites follow classic Langmuir-Hinshelwood kinetics, but under-coordinated sites follow non-standard kinetics with CO oxidation being barrierless but conversely also slow. This fundamental method of kinetic site deconvolution is broadly applicable to other catalytic systems, affording bridging of the complexity gap in heterogeneous catalysis.
Surface hydrophobization of zeolite enables mass transfer matching in gas-liquid-solid three-phase hydrogenation under ambient pressure
Authors: Wang Shuai , Hu Riming , Ren Jianyu , Lv Yipin , Song Lianghao , Zhao Huaiqing , Jiang Xuchuan , Gao Daowei , Chen Guozhu
Published: March 2024

Attaining high hydrogenation performance under mild conditions, especially at ambient pressure, remains a considerable challenge due to the difficulty in achieving efficient mass transfer at the gas-liquid-solid three-phase interface. Here, we present a zeolite nanoreactor with joint gas-solid-liquid interfaces for boosting H2 gas and substrates to involve reactions. Specifically, the Pt active sites are encapsulated within zeolite crystals, followed by modifying the external zeolite surface with organosilanes. The silane sheath with aerophilic/hydrophobic properties can promote the diffusion of H2 and the mass transfer of reactant/product molecules. In aqueous solutions, the gaseous H2 molecules can rapidly diffuse into the zeolite channels, thereby augmenting H2 concentration surround Pt sites. Simultaneously, the silane sheath with lipophilicity nature promotes the enrichment of the aldehydes/ketones on the catalyst and facilitates the hydrophilia products of alcohol rediffusion back to the aqueous phase. By modifying the wettability of the catalyst, the hydrogenation of aldehydes/ketones can be operated in water at ambient H2 pressure, resulting in a noteworthy turnover frequency up to 92.3 h−1 and a 4.3-fold increase in reaction rate compared to the unmodified catalyst.
A self-regulatory cell-wall-sensing module at cell edges controls plant growth
Authors: Elliott Liam , Kalde Monika , Schürholz Ann-Kathrin , Zhang Xinyu , Wolf Sebastian , Moore Ian , Kirchhelle Charlotte
Published: March 2024

Morphogenesis of multicellular organs requires coordination of cellular growth. In plants, cell growth is determined by turgor pressure and the mechanical properties of the cell wall, which also glues cells together. Because plants have to integrate tissue-scale mechanical stresses arising through growth in a fixed tissue topology, they need to monitor cell wall mechanical status and adapt growth accordingly. Molecular factors have been identified, but whether cell geometry contributes to wall sensing is unknown. Here we propose that plant cell edges act as cell-wall-sensing domains during growth. We describe two Receptor-Like Proteins, RLP4 and RLP4-L1, which occupy a unique polarity domain at cell edges established through a targeted secretory transport pathway. We show that RLP4s associate with the cell wall at edges via their extracellular domain, respond to changes in cell wall mechanics and contribute to directional growth control in Arabidopsis.
Ultra-sensitive, highly linear, and hysteresis-free strain sensors enabled by gradient stiffness sliding strategy
Authors: Xue Fuhua , Peng Qingyu , Ding Renjie , Li Pengyang , Zhao Xu , Zheng Haowen , Xu Liangliang , Tang Zhigong , Zhang Xinxing , He Xiaodong
Published: March 2024

Developing strain sensors with both high sensitivity and high linearity has always been the goal of researchers. Compared to resistive strain sensors, capacitive strain sensors have incomparable linearity advantages, but have always been limited by low sensitivity. Here, we report a gradient stiffness sliding design strategy that addresses this problem, significantly improving sensitivity while maintaining high linearity. By controlling the distribution of the locally enhanced electric field and the heterogeneous deformation of the substrate, a strain sensor with excellent performance is successfully prepared, exhibiting a giant gauge factor (9.1 × 106) and linearity (R2 = 0.9997) over the entire sensing range, together with almost no hysteresis and fast response time (17 ms). The gradient stiffness sliding design is a general strategy expected to be applied to other types of sensors to achieve ultra-high sensitivity and ultra-high linearity at the same time.
Morphological entropy encodes cellular migration strategies on multiple length scales
Authors: Liu Yanping , Jiao Yang , Fan Qihui , Li Xinwei , Liu Zhichao , Qin Dui , Hu Jun , Liu Liyu , Shuai Jianwei , Li Zhangyong
Published: March 2024

Cell migration is crucial for numerous physiological and pathological processes. A cell adapts its morphology, including the overall and nuclear morphology, in response to various cues in complex microenvironments, such as topotaxis and chemotaxis during migration. Thus, the dynamics of cellular morphology can encode migration strategies, from which diverse migration mechanisms can be inferred. However, deciphering the mechanisms behind cell migration encoded in morphology dynamics remains a challenging problem. Here, we present a powerful universal metric, the Cell Morphological Entropy (CME), developed by combining parametric morphological analysis with Shannon entropy. The utility of CME, which accurately quantifies the complex cellular morphology at multiple length scales through the deviation from a perfectly circular shape, is illustrated using a variety of normal and tumor cell lines in different in vitro microenvironments. Our results show how geometric constraints affect the MDA-MB-231 cell nucleus, the emerging interactions of MCF-10A cells migrating on collagen gel, and the critical transition from proliferation to invasion in tumor spheroids. The analysis demonstrates that the CME-based approach provides an effective and physically interpretable tool to measure morphology in real-time across multiple length scales. It provides deeper insight into cell migration and contributes to the understanding of different behavioral modes and collective cell motility in more complex microenvironments.
GOES-R land surface products at Western Hemisphere eddy covariance tower locations
Authors: Losos Danielle , Hoffman Sophie , Stoy Paul C.
Published: March 2024

The terrestrial carbon cycle varies dynamically on hourly to weekly scales, making it difficult to observe. Geostationary (“weather”) satellites like the Geostationary Environmental Operational Satellite - R Series (GOES-R) deliver near-hemispheric imagery at a ten-minute cadence. The Advanced Baseline Imager (ABI) aboard GOES-R measures visible and near-infrared spectral bands that can be used to estimate land surface properties and carbon dioxide flux. However, GOES-R data are designed for real-time dissemination and are difficult to link with eddy covariance time series of land-atmosphere carbon dioxide exchange. We compiled three-year time series of GOES-R land surface attributes including visible and near-infrared reflectances, land surface temperature (LST), and downwelling shortwave radiation (DSR) at 314 ABI fixed grid pixels containing eddy covariance towers. We demonstrate how to best combine satellite and in-situ datasets and show how ABI attributes useful for ecosystem monitoring vary across space and time. By connecting observation networks that infer rapid changes to the carbon cycle, we can gain a richer understanding of the processes that control it.