bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydroxypropyl Methylcellulose-Based Edible Coatings Formulated with Antifungal Food Additives to Reduce Alternaria Black Spot and Maintain Postharvest Quality of Cold-Stored ‘Rojo Brillante’ Persimmons
Authors: Fernández-Catalán Asunción , Palou Lluís , Taberner Verònica , Grimal Amparo , Argente-Sanchis Maricruz , Pérez-Gago María B.
Published: April 2021

Composite edible coatings based on hydroxypropyl methylcellulose (HPMC), as a polymeric phase, and oleic acid (OA) or beeswax (BW), as a hydrophobic phase, were formulated with different food additives as antifungal ingredients. HPMC–OA coatings containing 2% (w/v) sodium benzoate (SB), 1% ammonium carbonate (AC), 1% potassium carbonate (PC), 1% potassium bicarbonate (PBC), 1% sodium bicarbonate (SBC), 1% potassium silicate (PSi), 0.1% sodium methyl paraben (SMP) or 0.1% sodium ethyl paraben (SEP), and HPMC–BW coatings containing 2% sodium propionate (SP), 2% PBC, 2% SB or 0.1% SEP were evaluated for the control of Alternaria black spot (ABS) on Diospyros kaki Thunb. ’Rojo Brillante’ persimmons artificially inoculated with Alternaria alternata. After 14 days of incubation at 20 °C, HPMC–OA coatings formulated with PBC, PC or SEP were the most effective to reduce ABS incidence (61, 54, and 36% reduction, respectively, concerning uncoated control fruit) and severity (28, 12 and 22% reduction, respectively), while only HPMC–BW coatings formulated with SEP significantly reduced ABS incidence (50% reduction) and severity (36% reduction). HPMC–OA and HPMC–BW coatings containing 2% PBC or 0.1% SEP were selected to evaluate their effect on the weight loss, firmness and respiration rate of healthy ‘Rojo Brillante’ persimmons cold-stored at 1 °C and 90% relative humidity (RH) for 15 and 30 days, followed by 7 days of shelf life at 20 °C. HPMC–BW coatings were more effective in reducing fruit weight and firmness losses than HPMC–OA coatings, while all antifungal coatings significantly reduced fruit respiration. Overall, the HPMC–BW edible coating that contains SEP could be a promising postharvest treatment to control ABS and maintain the quality of cold-stored ‘Rojo Brillante’ persimmons.
Molecular Typing of Pathogenic Leptospira Species Isolated from Wild Mammal Reservoirs in Sardinia
Authors: Piredda Ivana , Ponti Maria Nicoletta , Palmas Bruna , Noworol Malgorzata , Pedditzi Aureliana , Rebechesu Lucio , Chisu Valentina
Published: April 2021

Leptospirosis is a global zoonosis caused by pathogenic species of Leptospira that infect a large spectrum of domestic and wild animals. This study is the first molecular identification, characterization, and phylogeny of Leptospira strains with veterinary and zoonotic impact in Sardinian wild hosts. All samples collected were cultured and analyzed by multiplex real time polymerase chain reaction (qPCR). Sequencing, phylogenetic analyses (based on rrs and secY sequences), and Multilocus Sequence Typing (MLST) based on the analysis of seven concatenated loci were also performed. Results revealed the detection of Leptospira DNA and cultured isolates in 21% and 4% of the samples examined, respectively. Sequence analysis of Leptospira positive samples highlighted the presence of the interrogans and borgpetersenii genospecies that grouped in strongly supported monophyletic clades. MLST analyses identified six different Sequence Types (ST) that clustered in two monophyletic groups specific for Leptospirainterrogans, and L. borgpetersenii. This study provided about the prevalence of leptospires in wild mammals in Sardinia, and increased our knowledge of this pathogen on the island. Monitoring Leptospira strains circulating in Sardinia will help clinicians and veterinarians develop strategic plans for the prevention and control of leptospiral infections.
Invited Review: Maintain or Improve Piglet Gut Health around Weanling: The Fundamental Effects of Dietary Amino Acids
Authors: Liao Shengfa F.
Published: April 2021

Gut health has significant implications for swine nutrient utilization and overall health. The basic gut morphology and its luminal microbiota play determinant roles for maintaining gut health and functions. Amino acids (AA), a group of essential nutrients for pigs, are not only obligatory for maintaining gut mucosal mass and integrity, but also for supporting the growth of luminal microbiota. This review summarized the up-to-date knowledge concerning the effects of dietary AA supplementation on the gut health of weanling piglets. For instance, threonine, arginine, glutamine, methionine and cysteine are beneficial to gut mucosal immunity and barrier function. Glutamine, arginine, threonine, methionine and cysteine can also assist with relieving the post-weaning stress of young piglets by improving gut immunological functions, antioxidant capacity, and/or anti-inflammatory ability. Glutamine, glutamate, glycine and cysteine can assist to reconstruct the gut structure after its damage and reverse its dysfunction. Furthermore, methionine, lysine, threonine, and glutamate play key roles in affecting bacteria growth in the lumen. Overall, the previous studies with different AA showed both similar and different effects on the gut health, but how to take advantages of all these effects for field application is not clear. It is uncertain whether these AA effects are synergetic or antagonistic. The interactions between the effects of non-nutrient feed additives and the fundamental effects of AA warrant further investigation. Considering the global push to minimize the antibiotics and ZnO usage in swine production, a primary effort at present may be made to explore the specific effects of individual AA, and then the concert effects of multiple AA, on the profile and functions of gut microbiota in young pigs.
Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
Authors: Coles Makenly E. , Forga Aaron J. , Señas-Cuesta Roberto , Graham Brittany D. , Selby Callie M. , Uribe Álvaro J. , Martínez Blanca C. , Angel-Isaza Jaime A. , Vuong Christine N. , Hernandez-Velasco Xochitl , Hargis Billy M. , Tellez-Isaias Guillermo
Published: April 2021

The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group (p < 0.05) compared with Group 2. Digested feed supernatant, supplemented with LEO and inoculated with CP, reduced CP burden (p < 0.05). Group 3 also exhibited a significant reduction in FITC-d, IFN-γ and IgA compared with Group 2. However, a significant increase SOD was observed in Group 3 compared with both control groups. Further investigation to compare the effect of LEO and the standard treatment of clostridial NE is required.
Lymphatic Drainage Mapping with Indirect Lymphography for Canine Mammary Tumors
Authors: Collivignarelli Francesco , Tamburro Roberto , Aste Giovanni , Falerno Ilaria , Del Signore Francesca , Simeoni Francesco , Patsikas Michail , Gianfelici Jacopo , Terragni Rossella , Attorri Valeria , Carluccio Augusto , Vignoli Massimo
Published: April 2021

Mammary gland tumors are the most common canine neoplasms. They account for 25–50% of all tumors diagnosed in bitches. Metastases and recurrences develop in about 35–70% of bitches following excision. The presence of regional lymph node metastases is a relevant factor affecting prognosis and treatment in cases of mammary gland tumors. The sentinel lymph node (SLN) is the first lymph node (or nodes) in the regional lymphatic basin that receives lymphatic flow from the primary neoplasm. The aim of this study is to investigate the SLN with indirect lymphography for a mammary tumor in dogs. The knowledge of the precise drainage pattern and SLN of the neoplastic mammary glands would provide clinically relevant information to the surgeon and to the oncologist, and it would be of high importance for the surgeon not only for performing the most adequate surgical excision but also for determining an accurate post-surgical prognosis.
Moringa oleifera Leaves as Eco-Friendly Feed Additive in Diets of Hy-Line Brown Hens during the Late Laying Period
Authors: Abdel-Wareth Ahmed A. A. , Lohakare Jayant
Published: April 2021

This study investigated the dietary effects of Moringa oleifera leaves supplementation on egg quality, laying performance, excreta ammonia concentrations and serum biochemistry of laying chickens during the late laying period. A total of 240 64-week-old Hy-Line Brown hens were assigned to four treatment diets including Moringa oleifera leaves at 0, 3, 6 or 9 g/kg, respectively, for eight weeks. The treatments had twelve replicates with five hens each. The results revealed that incremental dietary Moringa oleifera leaves significantly increased (p < 0.01) egg weight, production, and mass through 64–68, 68–72 and 64–72 weeks of age. Simultaneously, feed conversion ratio was significantly improved (p < 0.01) with Moringa oleifera leaves supplementation compared with the control. Haugh units and the thickness of eggshells significantly improved as a response to diets supplemented with 3, 6 and 9 g/kg Moringa oleifera leaves at 72 weeks of age. Interestingly, excreta ammonia concentrations, serum cholesterol, aspartate transaminase and alanine aminotransferase significantly decreased by Moringa oleifera leaves supplementation compared with the control group. In conclusion, introducing Moringa oleifera leaves supplementation at 3, 6 and 9 g/kg increased egg production, eggshell quality, Haugh units, and decreased serum cholesterol, triglycerides, excreta ammonia concentrations besides serum liver enzymes, uric acid and creatinine. Overall, based on the observed results, Moringa oleifera leaves supplementation was very promising and these leaves could be used as an effective feed additive in laying hens’ diet during the late laying period.
A Brief Overview of Oxidative Stress in Adipose Tissue with a Therapeutic Approach to Taking Antioxidant Supplements
Authors: Taherkhani Shima , Suzuki Katsuhiko , Ruhee Ruheea Taskin
Published: April 2021

One of the leading causes of obesity associated with oxidative stress (OS) is excessive consumption of nutrients, especially fast-foods, and a sedentary lifestyle, characterized by the ample accumulation of lipid in adipose tissue (AT). When the body needs energy, the lipid is broken down into glycerol (G) and free fatty acids (FFA) during the lipolysis process and transferred to various tissues in the body. Materials secreted from AT, especially adipocytokines (interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α)) and reactive oxygen species (ROS), are impressive in causing inflammation and OS of AT. There are several ways to improve obesity, but researchers have highly regarded the use of antioxidant supplements due to their neutralizing properties in removing ROS. In this review, we have examined the AT response to OS to antioxidant supplements focusing on animal studies. The results are inconsistent due to differences in the study duration and diversity in animals (strain, age, and sex). Therefore, there is a need for different studies, especially in humans.
The Effect of Different Sources of Selenium Supplementation on the Meat Quality Traits of Young Charolaise Bulls during the Finishing Phase
Authors: Grossi Silvia , Rossi Luciana , De Marco Michele , Sgoifo Rossi Carlo Angelo
Published: April 2021

The aim of the study was to compare the effects of sodium selenite (SS), selenium yeast (SY), and hydroxy-selenomethionine (OH-SeMet) on the meat quality and selenium (Se) deposition of finishing beef cattle. Sixty-three bulls were distributed over 3 treatments and fed SS, SY, or OH-SeMet at 0.2 mg kg−1 dry matter (DM) for 60 d. None of the Se sources affected the growth performance or carcass characteristics. OH-SeMet showed a higher Se transfer to the meat than SS or SY (p < 0.01). SY and OH-SeMet reduced the shear force of the meat (p < 0.0001), improved pH (p < 0.001), and reduced the drip losses (p < 0.001) and the lipid oxidation of the meat (p < 0.001). During 8 d of storage, OH-SeMet showed higher levels of meat lightness (L*) and yellowness (b*) than SS (p < 0.001), while the SY meat showed a higher L* than SS, albeit only on d 6. OH-SeMet improved b*, compared to SS, and also compared to SY on days 4, 7, and 8 (p < 0.001). Supplementing beef with SY and OH-SeMet improved several meat quality parameters. OH-SeMet appears to be the most effective strategy to improve the Se content and color stability of beef cattle meat.
Polyisocyanurate Foam Pyrolysis and Flame Spread Modeling
Authors: Chaudhari Dushyant M. , Stoliarov Stanislav I. , Beach Mark W. , Suryadevara Kali A.
Published: April 2021

Polyisocyanurate (PIR) foam is a robust thermal insulation material utilized widely in the modern construction. In this work, the flammability of one representative example of this material was studied systematically using experiments and modeling. The thermal decomposition of this material was analyzed through thermogravimetric analysis, differential scanning calorimetry, and microscale combustion calorimetry. The thermal transport properties of the pyrolyzing foam were evaluated using Controlled Atmosphere Pyrolysis Apparatus II experiments. Cone calorimetry tests were also carried out on the foam samples to quantify the contribution of the blowing agent (contained within the foam) to its flammability, which was found to be significant. A complete pyrolysis property set was developed and was shown to accurately predict the results of all aforementioned measurements. The foam was also subjected to full-scale flame spread tests, similar to the Single Burning Item test. A previously developed modeling approach based on a coupling between detailed pyrolysis simulations and a spatially-resolved relationship between the total heat release rate and heat feedback from the flame, derived from the experiments on a different material in the same experimental setup, was found to successfully predict the evolution of the heat release rate measured in the full-scale tests on the PIR foam.
The Design of Performance Guaranteed Autonomous Vehicle Control for Optimal Motion in Unsignalized Intersections
Authors: Németh Balázs , Gáspár Péter
Published: April 2021

The design of the motion of autonomous vehicles in non-signalized intersections with the consideration of multiple criteria and safety constraints is a challenging problem with several tasks. In this paper, a learning-based control solution with guarantees for collision avoidance is proposed. The design problem is formed in a novel way through the division of the control problem, which leads to reduced complexity for achieving real-time computation. First, an environment model for the intersection was created based on a constrained quadratic optimization, with which guarantees on collision avoidance can be provided. A robust cruise controller for the autonomous vehicle was also designed. Second, the environment model was used in the training process, which was based on a reinforcement learning method. The goal of the training was to improve the economy of autonomous vehicles, while guaranteeing collision avoidance. The effectiveness of the method is presented through simulation examples in non-signalized intersection scenarios with varying numbers of vehicles.
Performance Evaluation of rPPG Approaches with and without the Region-of-Interest Localization Step
Authors: Pirnar Žan , Finžgar Miha , Podržaj Primož
Published: April 2021

Traditionally, the first step in physiological measurements based on remote photoplethysmography (rPPG) is localizing the region of interest (ROI) that contains a desired pulsatile information. Recently, approaches that do not require this step have been proposed. The purpose of this study was to evaluate the performance of selected approaches with and without ROI localization step in rPPG signal extraction. The Viola-Jones face detector and Kanade–Lucas–Tomasi tracker (VK) in combination with (a) a region-of-interest (ROI) cropping, (b) facial landmarks, (c) skin-color segmentation, and (d) skin detection based on maximization of mutual information and an approach without ROI localization step (Full Video Pulse (FVP)) were studied. Final rPPG signals were extracted using selected model-based and data-driven rPPG algorithms. The performance of the approaches was tested on three publicly available data sets offering compressed and uncompressed video recordings covering various scenarios. The success rates of pulse waveform signal extraction range from 88.37% (VK with skin-color segmentation) to 100% (FVP). In challenging scenarios (skin tone, lighting conditions, exercise), there were no statistically significant differences between the studied approaches in terms of SNR. The best overall performance in terms of RMSE was achieved by a combination of VK with ROI cropping and the model-based rPPG algorithm. Results indicate that the selection of the ROI localization approach does not significantly affect rPPG measurements if combined with a robust algorithm for rPPG signal extraction.
A Novel Index to Detect Vegetation in Urban Areas Using UAV-Based Multispectral Images
Authors: Lee Geunsang , Hwang Jeewook , Cho Sangho
Published: April 2021

Unmanned aerial vehicles (UAVs) equipped with high-resolution multispectral cameras have increasingly been used in urban planning, landscape management, and environmental monitoring as an important complement to traditional satellite remote sensing systems. Interest in urban regeneration projects is on the rise in Korea, and the results of UAV-based urban vegetation analysis are in the spotlight as important data to effectively promote urban regeneration projects. Vegetation indices have been used to obtain vegetation information in a wide area using the multispectral bands of satellites. UAV images have recently been used to obtain vegetation information in a more rapid and precise manner. In this study, multispectral images were acquired using a UAV equipped with a Micasense RedEde MX camera to analyze vegetation indices, such as the Normalized Difference Vegetation Index (NDVI), Green Normalized Difference Vegetation Index (GNDVI), Blue Normalized Difference Vegetation Index (BNDVI), Red Green Blue Vegetation Index (RGBVI), Green Red Vegetation Index (GRVI), and Soil Adjusted Vegetation Index (SAVI). However, in the process of analyzing urban vegetation using the existing vegetation indices, it became clear that the vegetation index values of long-run steel roofing, waterproof coated roofs, and urethane-coated areas are often similar to, or slightly higher than, those of grass. In order to improve the problem of misclassification of vegetation, various equations were tested by combining multispectral bands. Kappa coefficient analysis showed that the squared Red-Blue NDVI index produced the best results when analyzing vegetation reflecting urban land cover. The novel vegetation index developed in this study will be very useful for effective analysis of vegetation in urban areas with various types of land cover, such as long-run steel roofing, waterproof coated roofs, and urethane-coated areas.
A Sensor Data-Driven Decision Support System for Liquefied Petroleum Gas Suppliers
Authors: Kozielski Michał , Henzel Joanna , Wróbel Łukasz , Łaskarzewski Zbigniew , Sikora Marek
Published: April 2021

Currently, efficiency in the supply domain and the ability to make quick and accurate decisions and to assess risk properly play a crucial role. The role of a decision support system (DSS) is to support the decision-making process in the enterprise, and for this, it is yet not enough to have up-to-date data; reliable predictions are necessary. Each application area has its own specificity, and so far, no dedicated DSS for liquefied petroleum gas (LPG) supply has been presented. This study presents a decision support system dedicated to support the LPG supply process from the perspective of gas demand analysis. This perspective includes a short- and medium-term gas demand prediction, as well as the definition and monitoring of key performance indicators. The analysis performed within the system is based exclusively on the collected sensory data; no data from any external enterprise resource planning (ERP) systems are used. Examples of forecasts and KPIs presented in the study show what kind of analysis can be implemented in the proposed system and prove its usefulness. This study, showing the overall workflow and the results for the use cases, which outperform the typical trivial approaches, could be a valuable direction for future works in the field of LPG and other fuel supply.
Strategy to Improve Edge-Based Smoothed Finite Element Solutions Using Enriched 2D Solid Finite Elements
Authors: Oh Min-Han , Kim San
Published: April 2021

In this paper, we present an automatic procedure that enhances the solution accuracy of edge-based smoothed 2D solid finite elements (three-node triangular and four-node quadrilateral elements). To obtain an enhanced solution, an adaptive enrichment scheme that uses enriched 2D solid finite elements and can effectively improve solution accuracy by applying cover functions adaptively without mesh-refinement is adopted in this procedure. First, the error of the edge-based finite element solution is estimated using a devised error estimation method, and appropriate cover functions are assigned for each node. While the edge-based smoothed finite elements provide piecewise constant strain fields, the proposed enrichment scheme uses the enriched finite elements to obtain a higher order strain field within the finite elements. Through various numerical examples, we demonstrate the accuracy improvement and efficiency achieved.
A Multi-Analytical Approach for the Characterization of Seventeenth Century Decorative Wall Paintings in Two Norwegian Stave Churches: A Case Study at Eidsborg and Heddal, Norway
Authors: Freeman Ashley Amanda , de Ferri Lavinia , Mazurek Joy , Andriulo Fabrizio , Bertolin Chiara
Published: April 2021

The presented research examines 17th century distemper paint from the polychrome wooden interiors of two Norwegian stave churches: Eidsborg and Heddal. For the first time, the inorganic and organic components of specimens from Eidsborg and Heddal were identified using X-ray Diffraction (XRD), Environmental Scanning Electron Microscopy (ESEM)—Energy Dispersive X-ray Spectroscopy (EDS), Fourier-Transform Infrared (FT-IR) spectroscopy, Enzyme-Linked Immunosorbent Assay (ELISA), and Gas Chromatography-Mass Spectrometry (GC-MS) after derivatization. This multi-analytical approach allowed for the identification of red ochre as the main red pigment within the topcoat (with the possible addition of minium), confirmed that a chalk basecoat was used, and finally permitted the recognition of alteration phases. Markers of proteinaceous material attributed to the use of animal-based glues were detected throughout the stratigraphic layers of both churches, with the addition of linseed oil in some locations. Furthermore, the wood substrate showed markers characteristic of pine tree, with contamination of wood fractions being detected in some of the paint samples from Heddal and Eidsborg. This research has contributed to a better understanding of the current preservation state of Heddal and Eidsborg, and ultimately assisted in developing a deeper comprehension and awareness of materials used in Norwegian stave churches.
Late Holocene Paleonvironmental Evolution of Two Coastal Lakes in Mediterranean Chile and Its Implications for Conservation Planning
Authors: Montes Isis-Yelena , Banegas-Medina Andy , Fagel Nathalie , El Ouahabi Meriam , Verleyen Elie , Alvarez Denisse , Torrejón Fernando , Schmidt Sabine , Lepoint Gilles , Diaz Gustavo , Pedreros Pablo , Urrutia Roberto
Published: April 2021

Paleolimnological reconstructions from the mid and high latitudes in the Southern Hemisphere are still relatively scarce. Anthropogenic impacts have evidenced trophic state changes and an increase in cyanobacterial blooms in the lacustrine system of San Pedro de la Paz in the last decades. Here, we reconstructed primary production and sedimentological changes spanning the past 2500 years in two coastal lakes in Mediterranean Chile. A multiproxy approach including sedimentological, biogenic silica, carbon and nitrogen isotopes and fossil pigments analysis in sediment cores was performed in Laguna Grande (LGSP) and Laguna Chica de San Pedro (LCSP). A marked change in the sedimentology of the lakes, likely related to the terrigenous sediment inputs derived by a transition from an arid condition in the mid-Holocene to a more humid condition in the late Holocene that favoured arboreal forest establishment at 100 BC–AD 150. A period of low primary production was identified between 850 to 1050 AC for LCSP, suggesting moist and cold conditions that were possibly related to La Niña events. In recent decades, there have been increases in primary production, probably resulting from anthropogenic disturbances. These likely include the clearance of native vegetation, the introduction of exotic tree species, and urbanisation, which in turn, resulted in nutrient inputs and hence eutrophication. We conclude that an integrated management program for both lakes is urgently needed.
Involvement of KL-6 Biomarker in Interstitial Lung Disease Induced by SARS-CoV-2 Infection: A Systematic Review
Authors: Crisan-Dabija Radu , Covic Adrian , Brinza Crischentian , Popa Iolanda Valentina , Burlacu Alexandru
Published: April 2021

Early prognosis of severe disease and preventive actions hang around as the mainstay in managing the novel SARS-COV-2 outbreak due to the lack of robust therapeutic strategies. Krebs von den Lungen-6 (KL-6 or KL-6/MUC1) is a relatively new discovered transmembrane mucoprotein that was shown to be a good predictor of disease severity in interstitial lung diseases (ILD). We aimed to systematically research the literature in order to assess the relationship between the KL-6 biomarker and prognosis of SARS-CoV-2 infection. A literature search was performed in PubMed, Embase, and Cochrane library databases from inception to 8 March 2021. After eligibility assessment, eight studies were finally included in the present systematic review. All included studies are observational and single-center. The data gathered suggests the importance of prognostic implications of KL-6 in COVID-19 as patients with a more severe disease had significantly higher levels of KL-6 at admission. Moreover, the KL-6 biomarker was associated with COVID-19 severity, lung lesion areas on computed tomography, pulmonary fibrosis, and coagulation disorders. The association with mortality is unclear and needs further research. More extensive trials are required to prove that facile, inexpensive, and good predictors of severe outcomes, such as KL-6, could be safely integrated into the clinical decision-making in patients with COVID-19.
Heterogeneity of Ex Vivo and In Vivo Properties along the Length of the Abdominal Aortic Aneurysm
Authors: Forneris Arianna , Nightingale Miriam , Ismaguilova Alina , Sigaeva Taisiya , Neave Louise , Bromley Amy , Moore Randy D. , Di Martino Elena S.
Published: April 2021

The current clinical guidelines for the management of aortic abdominal aneurysms (AAAs) overlook the structural and mechanical heterogeneity of the aortic tissue and its role in the regional weakening that drives disease progression. This study is a comprehensive investigation of the structural and biomechanical heterogeneity of AAA tissue along the length and circumference of the aorta, by means of regional ex vivo and in vivo properties. Biaxial testing and histological analysis were performed on ex vivo human aortic specimens systematically collected during open repair surgery. Wall-shear stress and three-dimensional principal strain analysis were performed to allow for in vivo regional characterization of individual aortas. A marked effect of position along the aortic length was observed in both ex vivo and in vivo properties, with the central regions corresponding to the aneurysmal sac being significantly different from the adjacent regions. The heterogeneity along the circumference of the aorta was reflected in the ex vivo biaxial response at low strains and histological properties. Present findings uniquely show the importance of regional characterization for aortic assessment and the need to correlate heterogeneity at the tissue level with non-invasive measurements aimed at improving clinical outcomes.
Water-Induced Inverse Correlation between Temperature and Flux Changes in Vertical Vapor-Phase Diffusive Transport of Volatile Organic Compounds in Near-Surface Soil Environments
Authors: Parlin Asma Akter , Watanabe Noriaki , Nakamura Kengo , Yamada Mizuki , Wang Jiajie , Komai Takeshi
Published: April 2021

Volatile organic compounds (VOCs) in contaminated soils have been investigated in near-surface environments to describe their transport behaviors and the resultant impacts of vapor intrusion into buildings. Prior studies have suggested that temperature changes significantly influence such transport behaviors in near-surface soils; however, the nature of these influences and their mechanisms have remained unclear. This is because an inverse correlation between the in situ temperature and VOC flux changes has been suggested but has not been experimentally investigated or demonstrated. Herein, we show the results of a set of experiments on the vertical and upward vapor-phase diffusive transport of benzene in sandy soils for different sand grain size and water content combinations under sinusoidal temperature changes between 20 and 30 °C. Under all experimental conditions, changes in the flux from the soil surface correlated with temperature changes, whereas changes in the flux into the overlying soil showed inverse correlations. Concurrent monitoring of the relative humidity revealed that an inverse correlation was exhibited in response to the condensation and volatilization of water. Moreover, the intensity of the inverse correlation was independent of grain size but was larger at higher water contents. Overall, water in soil may induce an inverse correlation.
Design Study of Steel Fibre Reinforced Concrete Shaft Lining for Swelling Ground in Toronto, Canada
Authors: Kim Min Seong , Lee Sean Seungwon
Published: April 2021

Reinforced concrete (RC) is a widely used construction material around the world. RC has many advantages in terms of structural stability. However, the reinforcement of RC requires extensive labour costs. Steel fibre reinforced concrete (SFRC) has been widely studied to replace steel bars in concrete structures over the decades. However, most underground structures, such as tunnel lining, are usually designed using conventional RC for long-term stability due to unexpected geotechnical characteristics, such as directional and depth-dependent varied lateral pressure, earthquakes, groundwater, and time-dependent swelling behaviour. In this paper, an alternative design of shaft structure using SFRC, based on the original RC designed data in the Toronto region, was studied to evaluate the feasibility of SFRC replacing conventional RC. A key geological feature of the site is that the bedrock is comprised of Georgian Bay shale, which exhibits long-term time-dependent deformation (TDD). The capacities of RC and SFRC for the shaft lining were calculated based on the Canadian concrete design codes CSA A23.3 and RILEM TC 162-TDF, to assess the benefit of adding steel fibre, and several analytical solutions were used to calculate the applied load on the lining. A specialised TDD constitutive model in Fast Lagrangian Analysis of Continua (FLAC) 2D was developed to estimate whether the optimum installation time of the shaft lining, based on the geological reports, is appropriate under swelling behaviour, and evaluate the resultant long-term stability. The calculated hoop thrust and bending moment for several loading cases were within the capacity of the SFRC shaft lining. The numerical analysis demonstrated that the proposed lining installation time could be reduced, despite consideration of the long-term TDD behaviour.
Applied Microbiology—An Open Access Journal
Authors: Connerton Ian
Published: April 2021

Welcome to Applied Microbiology, a new Open Access MDPI journal that will report original studies and reviews on the growing numbers of applications for microorganisms and their associated technologies [...]
Cyclone Signatures in the South-West Indian Ocean from Two Decades of Microseismic Noise
Authors: Rindraharisaona Elisa J. , Barruol Guilhem , Cordier Emmanuel , Fontaine Fabrice R. , Gonzalez Alicia
Published: April 2021

Tropical Cyclones (TC) represent the most destructive natural disaster affecting the islands in the South-West Indian Ocean (SWIO) each year. Monitoring ocean activity is therefore of primary importance to secure lands, infrastructures and peoples, but the little number of oceanographic instruments makes it challenging, particularly in real time. Long-term seismological records provide a way to decipher and quantify the past cyclonic activity by analyzing microseisms, seismic waves generated by the ocean activity and propagating through the solid Earth. In the present study, we analyze this microseismic noise generated by cyclones that develop in the SWIO basin between 1999 and 2020, using broadband seismic stations in La Réunion. The power spectral density (PSD), together with the root mean square (RMS) analyses of continuous seismic data recorded by the permanent Geoscope RER seismic station, indicate the intensification of the microseismic noise amplitude in proportion to the cyclone intensity. Thus, we establish a relationship between the cyclone intensity and the PSD of the Secondary Microseisms (SM) in frequency band ∼0.14 to 0.25 Hz (4 to 7 s period). The Pearson coefficient between the observed and estimated TC intensity are >0.8 in the presence of a cyclone with mean wind speeds >75 km/h and with a seismic station distance-to-storm center D < 3000 km. A polarization analysis in the time and frequency domains allows the retrieval of the backazimuth of the SM sources during isolated cyclone events and well-polarized signal, i.e., CpH > 0.6. We also analyzed the RMS of the Primary Microseisms (PM frequency between ∼0.05 and 0.1 Hz, i.e., for 10 to 20 s period) for cyclones passing nearby La Réunion (D < 500 km), using the available temporary and permanent broadband seismic stations. We also found high correlation coefficients (>0.8) between the PM amplitude and the local wave height issued from the global hindcast model demonstrating that the PM amplitude can be used as a robust proxy to perform a real-time wave-height monitoring in the neighboring ocean. Transfer functions are calculated for several cyclones to infer wave height from the seismic noise amplitude recorded on land. From the analysis of two decades of data, our results suggest that it is possible to quantify the past ocean activity for as long as continuous seismic archives are available, emphasizing microseismic noise as a key observable for quantifying and understanding the climate change.
Application of Morrison Cloud Microphysics Scheme in GRAPES_Meso Model and the Sensitivity Study on CCN’s Impacts on Cloud Radiation
Authors: Shi Yishe , Wang Hong , Shen Xinyong , Zhang Wenjie , Zhang Meng , Zhang Xiao , Peng Yue , Liu Zhaodong , Han Jing
Published: April 2021

In the present study, the Morrison double-moment cloud microphysics scheme including mass and droplet number concentration of water and ice clouds is implemented into the Chinese mesoscale version of the Global/Regional Assimilation and Prediction System (GRAPES_Meso). Sensitivity experiments of different cloud condensation nuclei (CCN) values are conducted to study the impacts of CCN on cloud microphysical processes and radiation processes in East China. The model evaluation shows that the simulated cloud liquid water path (CLWP) is consistent with that of the National Center for Environment Prediction (NCEP) reanalysis, and the cloud optical depth (COD) and effective radius of cloud water (Rc) are in agreement with those of the Moderate Resolution Imaging Spectroradiometer (MODIS) datasets both in regional distribution and magnitudes. These comparisons illustrate the effectiveness of the Morrison scheme for the cloud processes in East China. For the study period of 8 to 12 October 2017, the sensitivity experiments show that with initial CCN number concentration (CCN0) increasing from 10 to 3000 cm−3, the maximum value of daily average Rc decreases by about 63%, which leads to a decrease of cloud-rain conversion rate. Moreover, the maximum value of daily average mixing ratio of cloud water (qc) increases by 133%, the maximum value of daily average mixing ratio of rain (qr) decreases by 44%, and the maximum value of daily average CLWP and COD increase by 100% and 150%, respectively. This results in about 65% increasing of the maximum value of daily average cloud downward shortwave radiative forcing (CDSRF) when CCN0 increases from 10 cm−3 to 3000 cm−3. The study indicates the important impacts of CCN on cloud properties and radiation effects.
Chirality of Novel Bitopic Agonists Determines Unique Pharmacology at the Dopamine D3 Receptor
Authors: Adhikari Pramisha , Xie Bing , Semeano Ana , Bonifazi Alessandro , Battiti Francisco O. , Newman Amy H. , Yano Hideaki , Shi Lei
Published: April 2021

The dopamine D2/D3 receptor (D2R/D3R) agonists are used as therapeutics for Parkinson’s disease (PD) and other motor disorders. Selective targeting of D3R over D2R is attractive because of D3R’s restricted tissue distribution with potentially fewer side-effects and its putative neuroprotective effect. However, the high sequence homology between the D2R and D3R poses a challenge in the development of D3R selective agonists. To address the ligand selectivity, bitopic ligands were designed and synthesized previously based on a potent D3R-preferential agonist PF592,379 as the primary pharmacophore (PP). This PP was attached to various secondary pharmacophores (SPs) using chemically different linkers. Here, we characterize some of these novel bitopic ligands at both D3R and D2R using BRET-based functional assays. The bitopic ligands showed varying differences in potencies and efficacies. In addition, the chirality of the PP was key to conferring improved D3R potency, selectivity, and G protein signaling bias. In particular, compound AB04-88 exhibited significant D3R over D2R selectivity, and G protein bias at D3R. This bias was consistently observed at various time-points ranging from 8 to 46 min. Together, the structure-activity relationships derived from these functional studies reveal unique pharmacology at D3R and support further evaluation of functionally biased D3R agonists for their therapeutic potential.
The Body across the Lifespan: On the Relation between Interoceptive Sensibility and High-Order Body Representations
Authors: Raimo Simona , Di Vita Antonella , Boccia Maddalena , Iona Teresa , Cropano Maria , Gaita Mariachiara , Guariglia Cecilia , Grossi Dario , Palermo Liana
Published: April 2021

Background: Interoceptive information plays a pivotal role in building higher-order cognitive body representations (BR) that neuropsychological and neuroimaging evidence classifies as action-oriented (i.e., body schema) or non-action-oriented (i.e., visuo-spatial body map). This study aimed to explore the development of BR, considering the association with the interoceptive sensibility throughout the lifespan. Methods: Two hundred thirty-nine healthy participants divided into five age groups (7 to 8 years; 9 to 10 years; 18 to 40 years; 41 to 60 years; over 60 years) completed a self-report measure of interoceptive sensibility (the Self-Awareness Questionnaire; SAQ) and were given tasks assessing the two BR (action-oriented: hand laterality task; non-action-oriented: frontal body evocation task). Results: Both children (7–8 and 9–10 years) and older adults (over 60 years) performed worse than young (18–40 years) and middle-aged adults (41–60 years) in action- and non-action-oriented BR tasks. Moderation analyses showed that the SAQ score significantly moderated the relationship between age and action-oriented BR. Conclusions: The current results are consistent with inverted U-shaped developmental curves for action- and non-action-oriented BR. As an innovative aspect, the ability to mentally represent one’s own body parts in diverse states could be negatively affected by higher interoceptive sensibility levels in childhood and late adulthood.
Psychopathological Impact and Resilient Scenarios in Inpatient with Schizophrenia Spectrum Disorders Related to Covid Physical Distancing Policies: A Systematic Review
Authors: Caponnetto Pasquale , Benenati Alessandra , Maglia Marilena G.
Published: April 2021

The COVID-19 epidemic posed great challenges to the healthcare community. To contain the epidemiological emergency, confinement measures were instituted, affecting the entire population. The lack of social contact, as well as the disruption of daily life, caused the exacerbation of anxiety and depressive symptoms. The present review of the literature aims to investigate what the effects of the pandemic have been on patients with schizophrenia, hypothesizing, an exacerbation of psychotic symptomatology (positive, negative, disorganized symptoms). Between November 2020 and January 2021, 5353 articles were collected and analyzed from the databases of the ResearchGate, Pubmed, and Psycnet websites, subjected to PRISMA methodology. Of these, 11 were evaluated for eligibility, but only three were included in the study because they met all inclusion criteria. The research did not confirm the expected results, showing that any kind of worsening of schizophrenic symptomatology involved the study samples. However, interesting outcomes were highlighted, such as a significant increase in general well-being during the early period of the pandemic, especially by women, or an increase in CPR (C-reactive Protein) levels in the blood, signaling an inflammatory state. Although the systematic review refuted the initial hypothesis, this must be a starting point: the topic is recent and these findings leave ample room for further investigation, particularly in long-term longitudinal research. It is possible that the true response to this disruption of daily life that occurred only during the past year may manifest itself later in time. On the other hand, interesting outcomes have been brought to light that may provide further interesting research insights.
Effects of Coarse Aggregate Maximum Size on Synthetic/Steel Fiber Reinforced Concrete Performance with Different Fiber Parameters
Authors: Al-Baghdadi Haider M. , Al-Merib Faiz H. , Ibrahim Ayoob A. , Hassan Rafea F. , Hussein Husam H.
Published: April 2021

Recently, fiber has been incorporated into concrete mixtures, where its distribution in the concrete matrix helps to improve and enhance the mechanical properties of fiber-reinforced concrete (FRC). The aim of this study is to investigate the influence of steel and synthetic fiber parameters, along with different coarse aggregate maximum sizes (CAMZs) on FRC performance. Additionally, in past research, the empirical relationships among the compressive, tensile, and flexural strengths of plain concrete and FRC were assessed, and correlations between these mechanical properties of FRC were examined. For each CAMZ, four fiber dosages for each fiber type were considered. The results demonstrate the mechanical properties of FRC enhanced as the fiber length increased from 13 mm to 60 mm, the CAMZ increased from 9.5 mm to 37.5 mm, and the ratio of the fiber length to the CAMZ was in the range of 0.35–5.68. All mixtures have been intended to exhibit similar compressive strengths; however, the synthetic/steel fiber advanced the brittleness ratio of specimens with G10, G19, and G38 to approximately 36.8%, 40.7%, and 47.4% greater than the contral specimens, respectively. In addition, from the regression analysis investigation, there are strong correlations from the regression analysis of the mechanical property results of FRC.
Video-Laparoscopic versus Open Surgery in Obese Patients with Colorectal Cancer: A Propensity Score Matching Study
Authors: Bizzoca Cinzia , Zupo Roberta , Aquilino Fabrizio , Castellana Fabio , Fiore Felicia , Sardone Rodolfo , Vincenti Leonardo
Published: April 2021

Background: Minimally invasive surgery in obese patients is still challenging, so exploring one more item in this research field ranks among the main goals of this research. We aimed to compare short-term postoperative outcomes of open and video-laparoscopic (VL) approaches in CRC obese patients undergoing colorectal resection. Methods: We performed a retrospective analysis of a surgical database including 138 patients diagnosed with CRC, undergoing VL (n = 87, 63%) and open (n = 51, 37%) colorectal surgery. As a first step, propensity score matching was performed to balance the comparison between the two intervention groups (VL and open) in order to avoid selection bias. The matched sample (N = 98) was used to run further regression models in order to analyze the observed VL surgery advantages in terms of postoperative outcome, focusing on hospitalization and severity of postoperative complications, according to the Clavien–Dindo classification. Results: The study sample was predominantly male (N = 86, 62.3%), and VL was more frequent than open surgery (63% versus 37%). The two subgroup results obtained before and after the propensity score matching showed comparable findings for age, gender, BMI, and tumor staging. The specimen length and postoperative time before discharge were longer in open surgery (OS) patients; the number of harvested lymph nodes was higher than in VL patients as well (p < 0.01). Linear regression models applied separately on the outcomes of interest showed that VL-treated patients had a shorter hospital stay by almost two days and about one point less Clavien–Dindo severity than OS patients on average, given the same exposure to confounding variables. Tumor staging was not found to have a significant role in influencing the short-term outcomes investigated. Conclusion: Comparing open and VL surgery, improved postoperative outcomes were observed for VL surgery in obese patients after surgical resection for CRC. Both postoperative recovery time and Clavien–Dindo severity were better with VL surgery.