bluuebunny/crossref_metadata_2025 huggingface dataset:
Fine mapping and sequence analysis reveal a promising candidate gene encoding a novel NB-ARC domain derived from wild rice (Oryza officinalis) that confers bacterial blight resistance
Authors: Sinha Pragya , Kumar T. Dilip , Sk Hajira , Solanki Manish , Gokulan C. G. , Das Ayyappa , Miriyala Anila , Gonuguntala Rekha , Elumalai Punniakoti , M. B. V. N Kousik , S. K. Masthani , Kumboju Chaitra , Arra Yugander , G. S. Laha , Chirravuri N. Neerja , Patel Hitendra Kumar , Ghazi Irfan Ahmad , Kim Sung-Ryul , Jena Kshirod K. , Hanumanth Surekha Rani , Oliva Ricardo , Mangrauthia Satendra K. , Sundaram Raman Menakshi
Published: August 2023

Bacterial blight disease of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious constraints in rice production. The most sustainable strategy to combat the disease is the deployment of host plant resistance. Earlier, we identified an introgression line, IR 75084-15-3-B-B, derived from Oryza officinalis possessing broad-spectrum resistance against Xoo. In order to understand the inheritance of resistance in the O. officinalis accession and identify genomic region(s) associated with resistance, a recombinant inbred line (RIL) mapping population was developed from the cross Samba Mahsuri (susceptible to bacterial blight) × IR 75084-15-3-B-B (resistant to bacterial blight). The F2 population derived from the cross segregated in a phenotypic ratio of 3: 1 (resistant susceptible) implying that resistance in IR 75084-15-3-B-B is controlled by a single dominant gene/quantitative trait locus (QTL). In the F7 generation, a set of 47 homozygous resistant lines and 47 homozygous susceptible lines was used to study the association between phenotypic data obtained through screening with Xoo and genotypic data obtained through analysis of 7K rice single-nucleotide polymorphism (SNP) chip. Through composite interval mapping, a major locus was detected in the midst of two flanking SNP markers, viz., Chr11.27817978 and Chr11.27994133, on chromosome 11L with a logarithm of the odds (LOD) score of 10.21 and 35.93% of phenotypic variation, and the locus has been named Xa48t. In silico search in the genomic region between the two markers flanking Xa48t identified 10 putatively expressed genes located in the region of interest. The quantitative expression and DNA sequence analysis of these genes from contrasting parents identified the Os11g0687900 encoding an NB-ARC domain-containing protein as the most promising gene associated with resistance. Interestingly, a 16-bp insertion was noticed in the untranslated region (UTR) of the gene in the resistant parent, IR 75084-15-3-B-B, which was absent in Samba Mahsuri. The association of Os11g0687900 with resistance phenotype was further established by sequence-based DNA marker analysis in the RIL population. A co-segregating PCR-based INDEL marker, Marker_Xa48, has been developed for use in the marker-assisted breeding of Xa48t.
Genetic mapping of the LOSS OF PARTHENOGENESIS locus in Pilosella piloselloides and the evolution of apomixis in the Lactuceae
Authors: Bicknell Ross , Gaillard Marion , Catanach Andrew , McGee Robert , Erasmuson Sylvia , Fulton Beatrice , Winefield Christopher
Published: August 2023

Pilosella piloselloides var. praealta (syn. P. praealta; Hieracium praealtum) is a versatile model used to study gametophytic apomixis. In this system apomixis is controlled by three loci: one that controls the avoidance of meiosis (LOA), one that controls the avoidance of fertilization (LOP) and a third that controls autonomous endosperm formation (AutE). Using a unique polyhaploid mapping approach the LOP locus was mapped to a 654 kb genomic interval syntenic to linkage group 8 of Lactuca sativa. Polyhaploids form through the gametophytic action of a dominant determinant at LOP, so the mapped region represents both a functional and a physical domain for LOP in P. piloselloides. Allele sequence divergence (ASD) analysis of the PARTHENOGENESIS (PAR) gene within the LOP locus revealed that dominant PAR alleles in Pilosella remain highly similar across the genus, whilst the recessive alleles are more divergent. A previous report noted that dominant PAR alleles in both Pilosella and Taraxacum are modified by the presence of a class II transposable element (TE) in the promoter of the gene. This observation was confirmed and further extended to the related genus Hieracium. Sufficient differences were noted in the structure and location of the TE elements to conclude that TE insertional events had occurred independently in the three genera. Measures of allele crossover amongst the polyhaploids revealed that P. piloselloides is an autopolyploid species with tetrasomic inheritance. It was also noted that the dominant determinant of LOP in P. piloselloides could transmit via a diploid gamete (pollen or egg) but not via a haploid gamete. Using this information, a model is presented of how gametophytic apomixis may have evolved in several members of the Lactuceae, a tribe of the Asteraceae.
The impact of COVID-19 on students’ anxiety and its clarification: a systematic review
Authors: Wu Jiarun , Kuan Garry , Lou Hu , Hu Xiaoyu , Masri Mohamad Najmi , Sabo Abdulwali , Kueh Yee Cheng
Published: August 2023

Introduction

Since the emergence of COVID-19 in 2019, every country in the world has been affected to varying degrees. Long-term psychological pressure and anxiety will inevitably damage the physical and mental health of students. This study aimed to examine the effects of the COVID-19 pandemic on students who experienced stress and anxiety and to clarify which intervention was more effective.

Methods

A comprehensive literature search was conducted between January 2020 and December 2022 using online databases such as PubMed, Web of Science, Scopus, and Google Scholar by using the following keywords in combination: “COVID-19,” “stress,” “anxiety,” “depression,” and “intervention.” The retrieved literature was screened and reviewed.

Results

A total of 2,924 articles were retrieved using subject and keyword searches. After screening through the titles and abstracts, 18 related studies were retained. Their review revealed that: (1) most studies did not use medication to control stress and anxiety; (2) the standard methods used to reduce stress and anxiety were religion, psychological counseling, learning more about COVID-19 through the media, online mindfulness courses, improving sleep quality, and physical exercise; (3) the most effective interventions were physical activity and raising awareness about COVID-19 through the media and online mindfulness programs. However, some studies show that physical activity cannot directly relieve psychological stress and anxiety.

Conclusion

Limited interventions are effective, but learning more about COVID-19 and using active coping strategies may help reduce stress and anxiety. The implications of COVID-19 are also discussed.
Beyond the border of the athlete-centered approach: a model to understand runners' performance
Authors: Thuany Mabliny , Gomes Thayse Natacha , Weiss Katja , Knechtle Beat , Rolim Ramiro , Moura dos Santos Marcos André
Published: August 2023

Our purpose is to move beyond the borders of the athlete-centered approach by examining the runners' environment interplay as a key factor for performance. Based on the ecological systems theory, the micro-level (intrapersonal, interpersonal, and training characteristics), meso-level (a direct association with athletes is not observed, but the environment plays an influence on the relationships built at the micro-level), and macro-level (contextual features that influence athletic systems) were theorized and contextualized as important factors for the expression of different outcomes, including performance and participation. We also conceptualized the microtime, mesotime, and macrotime as a constraint. Through this model, we aimed to provide applications and conclusions about how this conceptual model provides advances in the scientific research field. By understanding how environmental factors influence their performance, runners can make informed decisions about where and how to train and compete. Furthermore, by recognizing the role of culture and social context in shaping runners' experiences and outcomes, we can work toward creating a more equitable and supportive running culture for all.
What complex factors influence sleep quality in college students? PLS-SEM vs. fsQCA
Authors: Wang Ying , Dai Xinyi , Zhu Jie , Xu Zeling , Lou Jiayao , Chen Keda
Published: August 2023

Introduction

Sleep quality has a significant impact on the health-related quality of life, particularly among college students. This study proposes a framework for identifying factors that influence college students’ sleep quality, including stress, self-control, bedtime habits, and neighborhood environment.

Methods

The study employed a cross-sectional analytical approach on a convenience sample of 255 medical students from a private university in China during the 2021/2022 academic year, of which 80.39% (205) were women. Two complementary methodologies, partial least squares-structural equation modeling (PLS-SEM), and fuzzy sets qualitative comparative analysis (fsQCA), were utilized in the study.

Results

The results of the PLS-SEM analysis suggest that Stress and Self-control act as mediating variables in the model, with Bedtime habits and Neighborhood environment influencing sleep quality through these variables. Additionally, the fsQCA analysis reveals that Bedtime habits and Neighborhood environment can combine with Stress and Self-control, respectively, to influence sleep quality.

Discussion

These findings provide insight into how multiple factors, such as Stress, Self-control, Bedtime habits, and Neighborhood environment, can impact college students’ sleep quality, and can be used to develop intervention programs aimed at improving it. Moreover, the use of both methodologies enables the expansion of new methodological approaches that can be applied to different contexts.
The overall and domain-specific quality of life of Chinese community-dwelling older adults: the role of intrinsic capacity and disease burden
Authors: Hu Xiuhua , Ruan Jian , Zhang Weibin , Chen Jie , Bao Zhijun , Ruan Qingwei , Yu Zhuowei
Published: August 2023

Objective

This study aimed to investigate the impact of the different domains of intrinsic capacity (IC) and chronic disease burden on health-related quality of life (HRQoL) and domain-specific HRQoL in Chinese community-dwelling older adults.

Design

A cross-sectional observational study of a community-based cohort.

Participants

We evaluated Chinese older adults (n = 429, mean age, 72.91 ± 7.014 years; female proportion, 57.30%).

Measurements

IC contains five domains, namely locomotion, vitality, cognition, psychological, and sensory capacity. Locomotion dysfunction was defined as grip and/or gait decline. Vitality decline was defined if two of the following three parameters were present: fatigue, physical inactivity, and weight loss or overweight. Cognition was classified into normal cognition, pre-mild cognitive impairment (pre-MCI), and MCI according to the normative z-scores of the neuropsychological test battery. Psychological dysfunction was diagnosed based on depressive symptoms. Sensory dysfunction was defined as hearing and/or vision impairment. HRQoL was assessed using the AQoL-8D scale, which comprised physical (including independent living, senses, and pain) and psychosocial (including mental health, happiness, self-worth, coping, and relationships) dimensions. Low HRQoL (HRQoL score or subscores in the highest quintile) was used as a dependent variable in logistic regression analyses adjusted for demographic, health-related, and psychological confounders.

Results

Sensory impairment was an independent determinant of senses, and locomotion impairment was significantly associated with overall HRQoL, independent living, and pain in the physical dimension of HRQoL. Cognition was an independent determinant of the senses. Vitality was independently associated with overall HRQoL, senses, and pain in the physical dimension and mental health and relationships in the psychological dimension of HRQoL. The psychological domain of IC was independently associated with overall and domain-specific HRQoL apart from senses after adjustment for all confounders. The number of multimorbidities mainly had a significant impact on independent living after adjustment for all confounders.

Conclusion

IC domains and chronic disease burden had heterogeneous influences on overall and domain-specific HRQoL. The impairment of sensory and locomotion domains had a synergistic impact on the overall and physical dimensions of HRQoL. The vitality and psychological domains of IC had more profound effects on HRQoL. Older people with high morbidity might have a higher risk of poor independent living.
Effects of red light on sleep and mood in healthy subjects and individuals with insomnia disorder
Authors: Pan Rong , Zhang Guimei , Deng Fangyi , Lin Weifeng , Pan Jiyang
Published: August 2023

Introduction

This study aimed to determine the influence of red light on objective sleep and the relationship between mood and sleep among individuals with insomnia disorder (ID).

Method

57 individuals with insomnia symptoms and 57 healthy participants were randomly divided into three groups (red- and white-light groups, and the black control group), which received different light treatments for 1 h before bedtime. The emotions and subjective alertness of participants were evaluated using Positive and Negative Affect Schedule scales (PANAS) and Karolinska Sleepiness Scale (KSS), their sleeping data were recorded using polysomnography (PSG).

Result

The negative emotion scores were higher in the healthy subject-red light (HS-RL) group than in the HS-white light (WL) and HS-black control (BC) groups (p < 0.001). The anxiety and negative emotion scores were higher in the ID-RL group than in the ID-WL and ID-BC groups (p = 0.007 and p < 0.001, respectively). The KSS scores were lower in the RL group than in the WL and BC groups for both HS and ID group (both p < 0.001). The SOL was shorter in the HS-RL group than in HS-WL group (p = 0.019). Compared with the HS-BC group, the HS-RL group had an increase in microarousal index (MAI) and N1% (p = 0.034 and p = 0.021, respectively), while the total sleep time (TST) and sleep efficiency (SE) decreased (p = 0.001 and p < 0.001, respectively). Compared with the ID-WL group, the SOL was shorter in the ID-RL group (p = 0.043), while TST, SE, number of microarousals (NMA), and numbers of cycles of REM period were increased (p = 0.016, p = 0.046, p = 0.001, and p = 0.041, respectively). Compared with the ID-BC group, the ID-RL group had increases in the SOL, WASO, and the numbers of cycles and NMA in REM period (p = 0.038, p = 0.005, p = 0.045, and p = 0.033, respectively), and a decrease in SE (p = 0.014). The effects of ID-WL (vs. ID-RL group) and ID-BC (vs. ID-RL group) on SOL were mediated by negative emotions (mediating effects were − 37.626 and − 33.768, respectively).

Conclusion

Red light can increase subjective alertness, anxiety, and negative emotions in both healthy subjects and people with ID, which can affect sleep directly or indirectly via the mediating effect of negative emotions.
Targeted health promotion with guided nature walks or group exercise: a controlled trial in primary care
Authors: Kolster Annika , Heikkinen Malin , Pajunen Adela , Mickos Anders , Wennman Heini , Partonen Timo
Published: August 2023

Background

Contact with nature promotes wellbeing through diverse pathways, providing a potential way of supporting health especially in primary care, where patients commonly suffer from multimorbidity and poor general health. Social prescribing is a non-pharmaceutical approach for improving health as well as social inclusion. This field study explores and compares the effects of a nature-based and an exercise-based social prescribing scheme on mental wellbeing and sleep, in a primary care population.

Methods

Primary care patients identified to benefit from a general improvement to their health were recruited by nurses, doctors, or social workers to this non-randomized, intention-to-treat, pilot field-study. Participants (n = 79) chose between the group interventions, either taking part in guided walks in nature, including immersion in a forest with high biodiversity, or participating in a versatile sports program. Mental wellbeing was assessed with the Warwick-Edinburgh Mental Well-Being Scale (WEMWBS), with additional questions evaluating self-rated health and sleep. Impact on mental wellbeing was explored in relation to perceived health. The amount and quality of sleep was measured with wrist-worn accelerometers. With a focus on everyday life impacts, the assessments took place before and after the 8-week intervention. All participants lived in Sipoo, Finland, an area with abundant accessible green space.

Results

Participants (mean age 57 years, 79% female) rated their general and mental health lower than the general population. Participation in the Nature-group resulted in improved mental wellbeing (change in WEMWBS by 3.15, p = 0.008), with a positive change for feeling relaxed, being cheerful, having energy to spare, feeling able to deal well with problems, feeling good about oneself and feeling close to other people. The Sports-group was beneficial for those initially rating their health as good. Sleep duration improved in the Sports-group, while participants in the Nature-group reported better sleep quality. Following the interventions there was improvement in perceived health and ability to function in both groups, while perceived mental health improved only in the Nature-group.

Conclusion

We attest that even in areas surrounded by greenery, active interventions can further improve health in a primary care population, and that nature-based interventions are beneficial for those in poor health.

Clinical trial registration

ClinicalTrials.gov, Identifier NCT05893212.
Digital health solution for monitoring and surveillance of Amyotrophic Lateral Sclerosis in Brazil
Authors: Barbalho Ingridy M. P. , Fonseca Aleika L. A. , Fernandes Felipe , Henriques Jorge , Gil Paulo , Nagem Danilo , Lindquist Raquel , Lima Thaisa , Santos João Paulo Queiroz dos , Paiva Jailton , Morais Antonio H. F. , Dourado Júnior Mário E. T. , Valentim Ricardo A. M.
Published: August 2023

Amyotrophic Lateral Sclerosis (ALS) is a complex and rare neurodegenerative disease given its heterogeneity. Despite being known for many years, few countries have accurate information about the characteristics of people diagnosed with ALS, such as data regarding diagnosis and clinical features of the disease. In Brazil, the lack of information about ALS limits data for the research progress and public policy development that benefits people affected by this health condition. In this context, this article aims to show a digital health solution development and application for research, intervention, and strengthening of the response to ALS in the Brazilian Health System. The proposed solution is composed of two platforms: the Brazilian National ALS Registry, responsible for the data collection in a structured way from ALS patients all over Brazil; and the Brazilian National ALS Observatory, responsible for processing the data collected in the National Registry and for providing a monitoring room with indicators on people diagnosed with ALS in Brazil. The development of this solution was supported by the Brazilian Ministry of Health (MoH) and was carried out by a multidisciplinary team with expertise in ALS. This solution represents a tool with great potential for strengthening public policies and stands out for being the only public database on the disease, besides containing innovations that allow data collection by health professionals and/or patients. By using both platforms, it is believed that it will be possible to understand the demographic and epidemiological data of ALS in Brazil, since the data will be able to be analyzed by care teams and also by public health managers, both in the individual and collective monitoring of people living with ALS in Brazil.
Dysmenorrhea in adolescents requires careful investigation of endometriosis—an analysis of early menstrual experiences in a large case-control study
Authors: El-Hadad Samia , Lässer Daksha , Sachs Maike-Katja , Schwartz Alexandra Sabrina Kohl , Haeberlin Felix , von Orelli Stephanie , Eberhard Markus , Leeners Brigitte
Published: August 2023

Introduction

Recent evidence shows that endometriosis, a significant cause of infertility, may already present in adolescents. Dysmenorrhea, often leading to school absences, is a key symptom of the maturing menstrual cycle but also of endometriosis. However, it is often perceived as “normal” and left untreated. In adolescents, laparoscopy, the standard procedure to diagnose endometriosis, is performed particularly cautiously. To improve reproductive health in adolescents, we evaluate associations between early menstrual experiences and endometriosis.

Methods

Retrospective data on early menstrual experiences from 563 women with surgically/histologically verified endometriosis and from 563 age-matched controls were compared. Study participants were recruited in Switzerland, Germany, and Austria. Information on menstrual experiences was collected via a structured questionnaire.

Results

The bivariate analysis showed that early menarche (p = 0.004), dysmenorrhea and negative memories of menarche (p < 0.001) were significantly associated with a diagnosis of endometriosis. After controlling for confounders in bivariate regression analysis occurrence of dysmenorrhea (p = <0.001, OR 5,74, 95% CI 3.82–7.22) especially with onset >3 years after menarche ((p = <0.001, OR 3.42, 95% CI 2.09–5.64) remained statistically significant predictors for diagnosis of endometriosis. Dysmenorrhea in mothers and mothers' perceived attitude towards menstruation were not associated with the occurrence of Endometriosis.

Conclusions

Dysmenorrhea and onset of dysmenorrhea at menarche or several years after it are strongly associated with the development of endometriosis. As mothers perceived attitudes towards menstruation show no significant association with their daughters' experiences, physical symptoms accompanying menarche and menstrual period pain in adolescents seem to be very reliable predictors in diagnosis of endometriosis. Therefore, dysmenorrhea in adolescents requires careful investigation of possible endometriosis, especially if it does not respond to medical management.

Clinical trials registration

The study was registered on ClinicalTrials.gov, identifier (NCT 02511626).
A pilot study of alterations of the gut microbiome in canine chronic kidney disease
Authors: Kim Kyung-Ryung , Kim Seon-Myung , Kim Jung-Hyun
Published: August 2023

Introduction

Gut dysbiosis has been noted in humans and animals with chronic kidney disease (CKD). However, little is known about the gut microbiome in canine patients with CKD. This study aimed to analyze and compare the gut microbiome profiles of healthy and CKD dogs, including differences in the gut microbiome between each CKD stage.

Methods

The study was conducted on 29 client-owned dogs who underwent physical examination, complete blood count (CBC), serum biochemistry, and urinalysis. The gut microbiome profile of healthy dogs (n = 10) and dogs with CKD (n = 19) was analyzed employing 16S rRNA sequencing.

Results

Significant differences were seen in the composition of the gut microbiome, with increased operational taxonomic units from the phylum Proteobacteria (p = 0.035), family Enterobacteriaceae (p < 0.001), and genus Enterococcus (p = 0.002) in dogs with CKD, and a decrease in the genus Ruminococcus (p = 0.007). Furthermore, an increase in both the progression of CKD and abundance of genus Klebsiella (Jonckheere-Terpstra test statistic value (JT) = 2.852, p = 0.004) and Clostridium (JT = 2.018, p = 0.044) was observed.

Discussion

Our study demonstrated that in dogs with CKD, the composition of the gut microbiome varied depending on the stage of CKD. Alterations in gut microbiome composition observed in CKD patients are characterized by an increase in proteolytic bacteria and a decrease in saccharolytic bacteria. These findings suggest specific gut microbiota could be targeted for clinical management of uremic dogs with CKD.
Franchisors’ Strategic Pricing Approaches for Franchise Fee Decisions and the Moderating Role of the Competitive Condition: Evidence from the Korean Franchising Market
Authors: Sun Kyung-A , Moon Joonho
Published: August 2023

As franchising provides several benefits to both corporations and small business owners, a growing number of contracts have been written through which corporations offer the right to use their brand name and business model, and small business owners pay fees for accepting the offers. In this franchisor–franchisee market, the franchise fee plays a pricing role in the exchange between two parties. In this context, we investigate the influence of franchisors’ strategic pricing approaches (i.e., cost- and value-based approaches) on franchise fee decisions. Furthermore, by examining the moderating effect of the competitive condition on the relationships between pricing approaches and franchise fees, we uncover franchisors’ pricing practices in greater detail. The results show that both pricing approaches have significant influences on franchise fee decisions, and the competitive condition moderates the relationship between the value-based approach and franchise fees but does not moderate the relationship between the cost-based approach and franchise fees. The findings contribute to the franchising and pricing literature and to industry practitioners.
Calibration of Collision Recovery Coefficient of Corn Seeds Based on High-Speed Photography and Sound Waveform Analysis
Authors: Li Xinping , Zhang Wantong , Xu Shendi , Ma Fuli , Du Zhe , Ma Yidong , Liu Jing
Published: August 2023

Compared with the movement of corn seeds in the sowing machine, the movement in the threshing machine is more intense. The collision between corn seeds and threshing parts and other corn seeds will not only change the movement path of corn seeds in the threshing clearance but also cause damage to corn seeds. Therefore, when using discrete element simulation technology to optimize the critical components of corn threshing machinery, it is necessary to measure corn seeds’ accurate collision recovery coefficient. However, when measuring the collision recovery coefficient between corn seeds, there will be multi-point collisions between corn seeds, affecting the measurement results’ accuracy. In order to solve this problem, this study combined high-speed photography and the sound waveform of corn seed collision to eliminate the interference of the multi-point collision phenomenon and improve the accuracy of measurement results. According to the above test method, the contact parameters of corn seeds were measured. Finally, the corn–corn rolling friction coefficient and corn–PMMA rolling friction coefficient were 0.0784 and 0.0934, respectively. The corn–corn static friction coefficient was 0.32, and the corn–PMMA static friction coefficient was 0.445. The corn–corn collision recovery and corn–PMMA collision recovery coefficients were 0.28 and 0.62, respectively. After that, the method’s reliability and the measurement results’ accuracy were verified by the plane collision test and repose angle test.
Multi-Parameter Health Assessment of Jujube Trees Based on Unmanned Aerial Vehicle Hyperspectral Remote Sensing
Authors: Wu Yuzhen , Zhao Qingzhan , Yin Xiaojun , Wang Yuanzhi , Tian Wenzhong
Published: August 2023

To address the current difficult problem of scientifically assessing the health status of date palm trees due to a single parameter for date palm health assessment, an imperfect index system, and low precision. In this paper, using jujube trees in 224 regiment of the 14th division of Xinjiang Production and Construction Corps “Kunyu city” as the research object, we carried out the inversion study of various physicochemical parameters of jujube trees (canopy chlorophyll content, leaf area index (LAI), tree height, canopy area) using the unmanned aerial vehicle (UAV) hyperspectral imagery of jujube trees during the period of fruit expansion, and put forward a model for assessing the health of jujube trees based on multiple physicochemical parameters. First, we calculated six spectral indices for inversion of chlorophyll content and four spectral index for inversion of LAI, analyzed the spectral index with high correlation with chlorophyll content and LAI of jujube trees canopy, and constructed the inversion models of chlorophyll content and LAI. Second, the Mask R-CNN model was used to achieve jujube trees’ canopy segmentation and area extraction, and the segmented canopy was matched with the Canopy Height Model (CHM) for jujube trees’ height extraction. Finally, based on the four physicochemical parameters of inversion, we construct four jujube trees’ health assessment models, namely, Partial Least Squares Regression Analysis (PLSR), Random Forest (RF), Support Vector Machines (SVM), and Decision Tree (DT). The results showed that the R2 of the PLSR tree health assessment model constructed based on the multi-physical and chemical parameters of chlorophyll content, LAI, tree height, and canopy area was 0.853, and the RMSE was 0.3. Compared with the jujube trees’ health assessment models constructed by RF, SVM, and DT, the R2 increased by 0.127, 0.386, and 0.165, and the RMSE decreased by 0.04, 0.175, and 0.063, respectively. This paper can achieve rapid and accurate inversion of multi-physical and chemical parameters of jujube trees with the help of UAV hyperspectral images, and the PLSR model constructed based on multi-physical and chemical parameters can accurately assess the health status of jujube trees and provide a reference for a scientific and reasonable assessment of jujube trees’ health.
Design and Field Test of a Leaping Type Soil-Covering Device on Plastic Film
Authors: Liu Quandong , Sun Wei , Wang Hucun , Meng Yangrong
Published: August 2023

This paper presents the designs and calculations of a leaping type soil-covering device for potato cultivation. The device is based on the full-film mulching cultivation mode, where the seed rows are covered with soil. The working principle of the leaping type soil-covering device was analyzed and the core structural parameters were determined. In order to search for a reasonable rotation speed of the screw conveyor, the soil-covering process of leaping type soil-covering device is simulated based on the EDEM software. Through single factor simulation experiments, relationship between the width and thickness of covering soil and the rotation speed of the screw conveyor was studied. The optimal values obtained were the scraper height of 60 mm and the screw conveyor rotation speed of 169 r/min. Based on the quadratic function fitting, the relationship between the length of the soil cover and the position of the seedling belt was determined. With the optimization objective of the seedling belt misalignment rate, the length of the soil cover was determined to be 417 mm. A prototype of the device was manufactured and tested in the field. The field test showed that the qualified rate of the thickness of the seeded soil cover was 92%, the qualified rate of covering soil thickness was 91%, the dislocation rate between the seeding row with covering soil and the seedling belt was 5%, and the damage degree of plastic film was 49.2 mm/m2. These results indicate that the designed potato planter with leaping type soil-covering device meets accepted agronomical requirements of potato planters and film-mulching planters. The natural emergence rate of potato seedlings reached 92% and the seedling burning rate was 7%.
Governance and Actions for Resilient Urban Food Systems in the Era of COVID-19: Lessons and Challenges in China
Authors: Xue Huidan , Zhai Yujia , Su Wen-Hao , He Ziling
Published: August 2023

The COVID-19 pandemic has drastically challenged urban food systems, has hurt the resilience and fundamental function of urban food systems and also accelerated the trends of digitization and changing preferences of consumers in cities. This research conducted a qualitative analysis of the discourses, actions and interactions of different actors in the urban food systems in China during COVID-19 using an actor-oriented approach and discourse analysis. This research finds that stricter regulations and policies have been implemented by governments to regulate the food supply chain and ensure human health. Local community service personnel, volunteers, stakeholders along the food supply chain and consumers formulated collective actions during the pandemic yet chaos and discourse distortions also emerged at different stages. The pandemic is a preamble to changes in consumers’ preferences and food supply chains in urban communities. There were significant structural changes and a dual structure of urban and rural food systems, where unbalanced supply and demand existed. Collective actions with community governance and an innovative food business model to digitize flows and easily adapt to shocks in food systems are required.
Characterization of Products from Catalytic Hydrothermal Carbonization of Animal Manure
Authors: Qaramaleki Saeed V. , Cardenas Jose , Jackson Michael A. , Compton David L. , Szogi Ariel A. , Ro Kyoung S. , Coronella Charles J.
Published: August 2023

This paper presents hydrothermal carbonization (HTC) of cow and pig manure in an acidic environment and compares the results with HTC performed without acid. HTC experiments were carried out at 170 °C for 10 min. The presence of an acid increased the extent of carbonization and energy densification for both types of manure. The heating value of the hydrochar was increased by an amount ranging from 9–21%, with sulfuric acid having a greater impact than acetic acid. The increased fuel densification may provide for more economic use as a fuel. Both acids increased the leaching of ionic species including PO4, Ca, K, Mg, and Fe. The results demonstrated conversion greater than 90% of total phosphorous to phosphate in hydrothermal conditions. HTC with sulfuric acid resulted in increased nitrogen leaching for both types of manure and resulted in the generation of ammonia. Further analysis of the aqueous products signified the formation of acetic acid, furfural, and 3-aminopyrazine 1-oxide compounds for the sulfuric acid-catalyzed hydrothermal treatment. Analysis of the residual gas confirmed the presence of a trace amount of H2S, NO2, and SO2 formation. The addition of sulfuric acid increased NO2 significantly. Finally, detailed mass balances are presented for carbon and macronutrients (NPK).
Aggregate-Breaking Mechanism Response to Polyacrylamide Application of Purple Soils in Southwestern China Using Le Bissonnais Method
Authors: Wang Yong , Wang Jiaqi , Ma Zhenzhen , Liang Xinlan
Published: August 2023

Polyacrylamide (PAM) is a water-soluble polymer with strong cohesiveness and a strong water absorption capacity, and it has been widely used to modify soil structural stability. However, little information is available on the impact of PAM application on the aggregate-breaking process of purple soils in hilly areas of southwestern China. Therefore, the current study aimed to examine the influence of PAM application on the aggregate stability of purple soil in terms of different breakdown mechanisms at different hillslope locations. Three disruptive tests employing the Le Bissonnais method (FW, fast-wetting sieving; SW, slow-wetting sieving; and WS, wet-stirring sieving) were used to determine the mean weight diameter (MWD), geometric mean diameter (GMD), and mass fractal dimension (D) of the soil aggregates, and soil erodibility factor (K) was calculated as an index of soil anti-erodibility. Overall, the major aggregate-breaking mechanism for purple soils was the following: SW (differential swelling) > WS (mechanical breakdown) > FW (slaking). The content of water-stable aggregates (>0.25 mm) obviously rose after PAM application, with the most significant influences shown under FW. A significant difference in MWD was observed between PAM application and without polyacrylamide application (CK) under WS (p < 0.05). However, there was a significant difference in GMD between PAM and CK (p < 0.05) under FW and SW. In comparison with CK, D value in PAM under FW and SW was significantly reduced, mainly at the slope locations of 0 and 20 m. A descending order of FW, WS, and SW was found on the basis of K value at different slope locations. These findings contribute to improved understanding of proper application of soil amendments to control soil and water loss in purple soils.
Root Architecture of Forage Species Varies with Intercropping Combinations
Authors: Liu Xindi , Jiao Yu , Zhao Xiaoyu , Yu Xiaoxu , Zhang Qingping , Li Shuo , Ma Lichao , Tang Wei , Yang Chao , Yang Guofeng , Sun Juan , Miao Fuhong
Published: August 2023

Belowground root systems under pasture intercropping exhibit complex interactions, and the root interactions of different intercropping combinations are still poorly understood. Therefore, in this work, two perennial and annual herbages were intercropped in pairs and evaluated at a ratio of 1:1. The root morphology and topological structure differed significantly with intercropping combinations. (1) Compared with other cropping patterns, the mean root diameter (RD) of intercropped alfalfa (Medicago sativa L.) and common vetch (Vicia sativa L.) increased notably. The root surface area (RSA), root volume (RV), and mean RD increased significantly when oat (Avena sativa L.) was intercropped with alfalfa. Similarly, the RSA and RV increased in intercropped oat, intercropping relative to monocropping. (2) The forage topological index of the intercropping system was close to one, which was close to that of the herringbone branching. Additionally, the intercropping system had a lower intensity of underground root competition. The root system of the different forage intercropping combinations tended to transition to dichotomous branching. (3) The correlations between root parameters differed according to forage species. Therefore, different intercropping combinations had different belowground root levels of competitiveness and interactions, thereby changing the resource competition environment.
Population Status and Vulnerability of Mantidactylus pauliani from Ankaratra Protected Area, Madagascar
Authors: Oninjatovo Radonirina Herizo , Randriamahatantsoa Bernard , Rabibisoa Nirhy H. C.
Published: August 2023

Mountain summits in Madagascar generally have species with specific habitat requirements, providing a home to a unique and locally endemic herpetofauna. Among them is M. pauliani, a typically aquatic and critically endangered amphibian found on the Ankaratra Massif. This species inhabits high elevations with a limited distribution range. Our study aimed to present new data on the distribution and elevational range, habitat use, and threats to M. pauliani and its occurrence according to habitat changes. To achieve this, annual monitoring was carried out from 2018 to 2021. Nine 100 m transects were established along streams at elevations ranging from 1762 to 2378 m a.s.l. along which we conducted visual encounter surveys. Data analysis was performed using a χ2 test and Factor Correspondence Analysis. We found that M. pauliani occupies elevations between 1900 and 2378 m a.s.l. within humid forests and savannah habitats. The results showed a fluctuation in the number of animals observed and a higher occurrence at higher elevations throughout the years according to the season, stream quality, and water volume. Ongoing habitat alteration makes M. pauliani vulnerable to population decline, with annual bushfires likely having a negative impact on habitat.
Are Dolphins Kept in Impoverished Environments?
Authors: Jaakkola Kelly
Published: August 2023

Numerous studies have demonstrated the negative effects of impoverished environments versus the positive effects of enriched environments on animals’ cognitive and neural functioning. Recently, a hypothesis was raised suggesting that conditions for dolphins in zoological facilities may be inherently impoverished, and thus lead to neural and cognitive deficits. This review directly examines that hypothesis in light of the existing scientific literature relevant to dolphin welfare in zoological facilities. Specifically, it examines how dolphins are housed in modern zoological facilities, where the characteristics of such housing fall on the continuum of impoverished-to-enriched environments, and the extent to which dolphins show behavioral evidence characteristic of living in impoverished environments. The results of this analysis show that contrary to the original hypothesis, modern zoological facilities do not inherently, or even typically, house dolphins in impoverished conditions. However, it also notes that there is variation in animal welfare across different zoological facilities, and that “not impoverished” would be a particularly low bar to set as an animal welfare standard. To optimize cognitive well-being, strategies for providing additional cognitive challenges for dolphins in zoological facilities are suggested.
The Use of Medical Grade Honey on Infected Chronic Diabetic Foot Ulcers—A Prospective Case-Control Study
Authors: Holubová Adéla , Chlupáčová Lucie , Krocová Jitka , Cetlová Lada , Peters Linsey J. F. , Cremers Niels A. J. , Pokorná Andrea
Published: August 2023

Non-healing wounds are usually colonised and contaminated by different types of bacteria. An alternative to antibiotic treatment in patients with infected wounds with local signs of inflammation may be medical grade honey (MGH). MGH has antioxidant, antimicrobial, anti-inflammatory, and immunomodulatory features. This study aims to evaluate the effect of MGH therapy on infected non-healing wounds, especially for diabetic foot syndrome. Prospective, observational case series (n = 5) of patients with wounds of diabetic foot syndrome are presented. There were five males with an average age of 61.6 years. All wounds were treated with MGH, and the healing trajectory was rigorously and objectively monitored. In all cases, there was a gradual disappearance of odour, pain, and exudation. Moreover, the wound areas significantly reduced within 40 days and there was a decrease in glycated haemoglobin and glycaemia values. All these outcomes resulted in improved quality of life of the patients. Despite bacterial colonisation, antibiotic treatment was not necessary. All wounds were completely healed. MGH has antimicrobial, anti-inflammatory, and antioxidant effects in diabetic foot syndrome wounds, does not increase glycated haemoglobin or glycaemia levels, and thus constitutes an effective alternative to the use of antibiotics in the treatment of locally infected wounds.
Horizontal Gene Transfer and Drug Resistance Involving Mycobacterium tuberculosis
Authors: Xia Xuhua
Published: August 2023

Mycobacterium tuberculosis (Mtb) acquires drug resistance at a rate comparable to that of bacterial pathogens that replicate much faster and have a higher mutation rate. One explanation for this rapid acquisition of drug resistance in Mtb is that drug resistance may evolve in other fast-replicating mycobacteria and then be transferred to Mtb through horizontal gene transfer (HGT). This paper aims to address three questions. First, does HGT occur between Mtb and other mycobacterial species? Second, what genes after HGT tend to survive in the recipient genome? Third, does HGT contribute to antibiotic resistance in Mtb? I present a conceptual framework for detecting HGT and analyze 39 ribosomal protein genes, 23S and 16S ribosomal RNA genes, as well as several genes targeted by antibiotics against Mtb, from 43 genomes representing all major groups within Mycobacterium. I also included mgtC and the insertion sequence IS6110 that were previously reported to be involved in HGT. The insertion sequence IS6110 shows clearly that the Mtb complex participates in HGT. However, the horizontal transferability of genes depends on gene function, as was previously hypothesized. HGT is not observed in functionally important genes such as ribosomal protein genes, rRNA genes, and other genes chosen as drug targets. This pattern can be explained by differential selection against functionally important and unimportant genes after HGT. Functionally unimportant genes such as IS6110 are not strongly selected against, so HGT events involving such genes are visible. For functionally important genes, a horizontally transferred diverged homologue from a different species may not work as well as the native counterpart, so the HGT event involving such genes is strongly selected against and eliminated, rendering them invisible to us. In short, while HGT involving the Mtb complex occurs, antibiotic resistance in the Mtb complex arose from mutations in those drug-targeted genes within the Mtb complex and was not gained through HGT.
Detection Method of Partial Discharge on Transformer and Gas-Insulated Switchgear: A Review
Authors: Faizol Zulbirri , Zubir Farid , Saman Norhafezaidi Mat , Ahmad Mohd Hafizi , Rahim Mohamad Kamal A. , Ayop Osman , Jusoh Muzammil , Majid Huda A. , Yusoff Zubaida
Published: August 2023

The detection of partial discharge (PD) activities in high-voltage equipment can be conducted according to several mechanisms of signal detection, including electromagnetic wave signal detection, acoustic signal detection, chemical reactions, electrical signal detection, and optical emission detection. Recently, multiple methods of detection and localization of partial discharge activities, which occurred in power transformers and gas-insulated switchgear (GIS), have been proposed to monitor the health condition of high-voltage equipment, especially when the awareness regarding preventive maintenance has been emphasized at the industrial level and among electrical providers. In aligning the needs of the industrial sector and the improvement of PD-detection methods, this manuscript focuses on reviewing the current practice methods for the detection and localization of PD signals in high-voltage equipment, comparing their efficacy, and summarizing the future direction of research work-related methods of PD detection. The comparative reviews are discussed in terms of the mechanism of PD signal detection, indication parameters, calibration techniques, and the advantages and limitations of each method of PD measurement in detail.
High-Aspect-Ratio Silicon Metasurfaces: Design, Fabrication, and Characterization
Authors: Yao Yao , Wang Zhuo , Zhu Zhiyan , He Yu , Sun Shulin , Zhou Lei , He Qiong
Published: August 2023

Unrestricted manipulations on terahertz (THz) waves are highly desired in integration-optics, but THz devices based on conventional materials are usually bulky in size. Although all-silicon metasurfaces have exhibited great capabilities in manipulating THz waves, most of them are less efficient and have limited functionalities. In this work, we first design a silicon meta-atom structure consisting of a high-aspect-ratio (AR) micro-pillar that exhibits nearly perfect transmission and large transmission phase of THz wave, and systemically study how the fabrication quality (e.g., steepness of the sidewall and the vertical thickness distribution) may influence the final performance of a functional metasurface constructed with such meta-atoms. After experimentally examining how two working phases in the deep-reactive-ion-etch technology (i.e., the etch and passivation phases) influence the quality of the fabricated meta-devices, we develop an optimized Bosch process to realize high-AR (~20:1) all-silicon metasurfaces by balancing two working phases. We finally design/fabricate a high-AR silicon metasurface and experimentally demonstrate that it behaves as a high-efficiency half-wave-plate for THz waves in transmission geometry. Our results pave the avenue to realize ultra-compact THz meta-devices with high performance in transmission geometry, which is highly desirable for THz applications.