bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of High-Frequency Oscillation in Locating an Epileptogenic Zone for Radiofrequency Thermocoagulation
Authors: Xu Xin , Yu Xingguang , Kang Guixia , Mao Zhiqi , Cui Zhiqiang , Pan Longsheng , Zong Rui , Tang Yuan , Wan Ming , Ling Zhipei
Published: September 2021

Radiofrequency thermocoagulation (RFTC) has been proposed as a first-line surgical treatment option for patients with drug-resistant focal epilepsy (DRE) that is associated with gray matter nodular heterotopia (GMNH). Excellent results on seizures have been reported following unilateral RFTC performed on ictal high-frequency-discharge, fast-rhythm, and low-voltage initiation areas. Complex cases (GMNH plus other malformations of cortical development) do not have good outcomes with RFTC. Yet, there is little research studying the effect of high-frequency oscillation in locating epileptogenic zones for thermocoagulation on unilateral, DRE with bilateral GMNH. We present a case of DRE with bilateral GMNH, treated using RFTC on unilateral GMNH and the overlying cortex, guided by stereotactic electroencephalogram (SEGG), and followed up for 69 months. Twenty-four-hour EGG recordings, seizure frequency, post-RFTC MRI, and neuropsychological tests were performed once yearly. To date, this patient is seizure-free, the electroencephalogram is normal, neuropsychological problems have not been found, and the trace of RFTC has been clearly identified on MRI. His dosage of antiepileptic medication has, furthermore, been significantly reduced. It is concluded that RFTC on unilateral DRE with bilateral GMNH may achieve good long-term effects, lasting up to, and perhaps longer than, 69 months. Ictal high-frequency oscillation (fast ripple) inside the heterotopia and the overlying cortex may be the key to this successful effect.
Clinical Characteristics of Pediatric Cases of COVID-19 in Hunan, China: A Retrospective, Multi-Center Case Series
Authors: Wu Lei , Zhang Xiao-Fo , Yang Yong , Yi Xiu-Ying , Jiang Xin-Ping , Han Hai-Ying , Cao Xiao-Ying , Wang Tuan-Mei , Wang Man-Zhi
Published: September 2021

Objective: Aimed to investigate the epidemiological characteristics, clinical features, treatment, and short-term prognosis of COVID-19 in children.

Methods: Retrospective analysis was conducted in 48 children with COVID-19 admitted to 12 hospitals in eight cities in Hunan province, China, from January 26, 2020 to June 30, 2020.

Results: Of the 48 cases, Familial clusters were confirmed for 46 children (96%). 16 (33%) were imported from other provinces. There were 11 (23%) asymptomatic cases. only 2 cases (4%) were severe. The most common symptom was fever (n = 20, 42%). Other symptoms included cough (n = 19, 40%), fatigue (n = 8, 17%), and diarrhea (n = 5, 10%). In the early stage, the total peripheral blood leukocytes count increased in 3(6%) cases and the lymphocytes count decreased in 5 (10%) cases. C-reactive protein and procalcitonin were elevated respectively in 3 (6%) cases and 2 (4%) cases. There were abnormal chest CT changes in 22 (46%) children, including 15 (68%) with patchy ground glass opacity, 5 (22%) with consolidation, and 2 (10%) with mixed shadowing. In addition to supportive treatment, antiviral therapy was received by 41 (85%) children, 11 (23%) patients were treated with antibiotics, and 2 (4%) were treated with methylprednisolone and intravenous immunoglobulin. Compared to 2 weeks follow-up, one child developed low fever and headache during the 4 weeks follow-up, 3 (6%) children had runny noses, one of them got mild cough, and 4 (12%) children had elevated white blood cells and lymphocytes. However, LDH and CK increased at 2 weeks and 4 weeks follow-up. 2 weeks follow-up identified normal chest radiographs in 33 (69%) pediatric patients. RT-PCR detection of SARS-CoV-2 was negative in all follow-up patients at 2 and 4 weeks follow-up. All 48 pediatric patients were visited by calling after 1 year of discharge.

Conclusions: Most cases of COVID-19 in children in Hunan province were asymptomatic, mild, or moderate. Close family contact was the main route of infection. It appeared that the younger the patient, the less obvious their symptoms. Epidemiological history, nucleic acid test, and chest imaging were important tools for diagnosis in children.
Protective Effects of Lentinan Against Lipopolysaccharide-Induced Mastitis in Mice
Authors: Yin Huifang , Xue Guanhong , Dai Ailing , Wu Haichong
Published: September 2021

Mastitis is a worldwide production disease in dairy cows, which mainly affects milk yield, causing huge economic losses to dairy farmers. Lentinan is a kind of polysaccharide extracted from Lentinus edodes, which has no toxicity and possesses various pharmacological activities including antibacterial and immunomodulatory effects. Therefore, the anti-inflammatory function of lentinan on LPS-stimulated mastitis was carried out, and the mechanism involved was explored. In vivo, lentinan greatly reduced LPS-stimulated pathological injury, myeloperoxidase (MPO) activity, and the proinflammatory factor production (TNF-α and IL-1β) in mice. Further study was performed to determine the activation of the Wnt/β-catenin pathway during LPS stimulation. These results suggested that LPS-induced activation of the Wnt/β-catenin pathway was suppressed by lentinan administration. In vitro, we observed that the mouse mammary epithelial cell (mMEC) viability was not affected by lentinan treatment. As expected, LPS increased the TNF-α and IL-1β protein secretion and the activation of the Wnt/β-catenin pathway that was inhibited by lentinan administration in a dose-dependent manner in mMECs. Conclusively, lentinan exerts the anti-inflammatory function in LPS-stimulated mastitis via inhibiting the activation of the Wnt/β-catenin pathway. Thus, the results of our study also gave an insight that lentinan may serve as a potential treatment for mastitis.
Spatial Changes in the Atrial Fibrillation Wave-Dynamics After Using Antiarrhythmic Drugs: A Computational Modeling Study
Authors: Hwang Inseok , Jin Ze , Park Je-Wook , Kwon Oh-Seok , Lim Byounghyun , Lee Jisu , Yu Hee-Tae , Kim Tae-Hoon , Joung Boyoung , Pak Hui-Nam
Published: September 2021

Background: We previously reported that a computational modeling-guided antiarrhythmic drug (AAD) test was feasible for evaluating multiple AADs in patients with atrial fibrillation (AF). We explored the anti-AF mechanisms of AADs and spatial change in the AF wave-dynamics by a realistic computational model.

Methods: We used realistic computational modeling of 25 AF patients (68% male, 59.8 ± 9.8 years old, 32.0% paroxysmal AF) reflecting the anatomy, histology, and electrophysiology of the left atrium (LA) to characterize the effects of five AADs (amiodarone, sotalol, dronedarone, flecainide, and propafenone). We evaluated the spatial change in the AF wave-dynamics by measuring the mean dominant frequency (DF) and its coefficient of variation [dominant frequency-coefficient of variation (DF-COV)] in 10 segments of the LA. The mean DF and DF-COV were compared according to the pulmonary vein (PV) vs. extra-PV, maximal slope of the restitution curves (Smax), and defragmentation of AF.

Results: The mean DF decreased after the administration of AADs in the dose dependent manner (p < 0.001). Under AADs, the DF was significantly lower (p < 0.001) and COV-DF higher (p = 0.003) in the PV than extra-PV region. The mean DF was significantly lower at a high Smax (≥1.4) than a lower Smax condition under AADs. During the episodes of AF defragmentation, the mean DF was lower (p < 0.001), but the COV-DF was higher (p < 0.001) than that in those without defragmentation.

Conclusions: The DF reduction with AADs is predominant in the PVs and during a high Smax condition and causes AF termination or defragmentation during a lower DF and spatially unstable (higher DF-COV) condition.
Insomnia Promotes Hepatic Steatosis in Rats Possibly by Mediating Sympathetic Overactivation
Authors: Wang Zongding , Liang Xiaoyan , Lu Yanmei , Jiang Tiemin , Aji Tuerganaili , Aimulajiang Kalibixiati , Sun Huaxin , Zhang Ling , Zhou Xianhui , Tang Baopeng , Wen Hao
Published: September 2021

Background: Insomnia is a widespread problem that can lead to the occurrence of other diseases and correlates closely with sympathetic nerve hyperactivation. Obesity-induced hepatic steatosis is mediated by sympathetic overactivation. However, it remains unclear whether insomnia may cause hepatic steatosis. The goal of this study was to preliminarily investigate whether insomnia caused hepatic steatosis in rats via sympathetic hyperactivation.

Methods: A total of 32 Sprague-Dawley male rats were divided randomly into four groups: model, sympathetic denervation (Sd), estazolam, and control (eight rats/group). Model group received sustained sleep deprivation using the modified multiple platform method. In the Sd group, rats underwent sleep deprivation after receiving Sd by 6-hydroxydopamine (6-OHDA). Estazolam group: the rats concurrently received sleep deprivation and treatment with estazolam. The other eight rats housed in cages and kept in a comfortable environment were used as control. Blood samples were obtained for analysis of plasma lipids and hepatic function. Sympathetic hyperactivation-related indexes and hepatic steatosis in liver tissues were tested.

Results: Liver enzymes, plasma lipid levels, and hepatic steatosis were elevated in insomnia rats, and sympathetic hyperactivation was found. Insomnia-induced hepatic steatosis was effectively lowered with pharmacological ablation of the hepatic sympathetic nerves. Furthermore, the treatment of insomnia with estazolam inhibited sympathetic activation and reduced hepatic steatosis.

Conclusion: Sustained sleep deprivation-induced insomnia promotes hepatic steatosis in rats possibly by mediating sympathetic overactivation.
Estrogen Receptor and Vascular Aging
Authors: Davezac Morgane , Buscato Melissa , Zahreddine Rana , Lacolley Patrick , Henrion Daniel , Lenfant Francoise , Arnal Jean-Francois , Fontaine Coralie
Published: September 2021

Cardiovascular diseases remain an age-related pathology in both men and women. These pathologies are 3-fold more frequent in men than in women before menopause, although this difference progressively decreases after menopause. The vasculoprotective role of estrogens are well established before menopause, but the consequences of their abrupt decline on the cardiovascular risk at menopause remain debated. In this review, we will attempt to summarize the main clinical and experimental studies reporting the protective effects of estrogens against cardiovascular diseases, with a particular focus on atherosclerosis, and the impact of aging and estrogen deprivation on their endothelial actions. The arterial actions of estrogens, but also part of that of androgens through their aromatization into estrogens, are mediated by the estrogen receptor (ER)α and ERβ. ERs belong to the nuclear receptor family and act by transcriptional regulation in the nucleus, but also exert non-genomic/extranuclear actions. Beside the decline of estrogens at menopause, abnormalities in the expression and/or function of ERs in the tissues, and particularly in arteries, could contribute to the failure of classic estrogens to protect arteries during aging. Finally, we will discuss how recent insights in the mechanisms of action of ERα could contribute to optimize the hormonal treatment of the menopause.
Relationships between the Big-5 Model and Effectuation versus Causation Logics of Entrepreneurs in New Ventures: The Estonian IT Sector
Authors: Coudounaris Dafnis , Arvidsson Henrik
Published: September 2021

This study aims to investigate the influence of the big-5 personality traits on causation and effectuation decision-making logics using the entrepreneurial process theory. This is an empirical study based on 113 surveys of managers/entrepreneurs from the Estonian IT sector. The questionnaire was uploaded onto the online platform of connect.ee and the participants were invited to complete it. The study reveals that only conscientiousness positively and significantly predicts causation logic. However, four of the five factors of personality traits positively and significantly predict effectuation logic, i.e., openness, conscientiousness, extraversion, agreeableness, except for neuroticism. Furthermore, only nine of 25 hypotheses are positive and significant, and of the 11 relationships of the model, three are negative and non-significant for causation logic and eight are negative and non-significant for effectuation logic. Finally, there are another five relationships of the model which are positively but non-significantly related. Managers of IT companies in Estonia should think of shifting to effectuation logic as most personality traits predict effectuation logic, and therefore, there is the possibility of a better performance for IT firms. Eight of the 51 items of the model had standardised regression weights below the threshold of 0.500, but only four were extracted from the final model. The extraction of items from the model indicates the need for the re-identification of the constructs of personality traits using, for example, the six-factor personality traits.
Introducing Grasslands into Crop Rotations, a Way to Restore Microbiodiversity and Soil Functions
Authors: Riah-Anglet Wassila , Cusset Elodie , Chaussod Rémi , Criquet Stéven , Norini Marie-Paule , Cheviron Nathalie , Mougin Christian , Laval Karine , Latour Xavier , Legras Marc , Trinsoutrot-Gattin Isabelle
Published: September 2021

The aims of this study were to investigate (i) the influence of aging grassland in the recovery of soil state by the comparison of permanent grassland, two restored grasslands, two temporary grasslands, and a continuous crop in the same pedoclimatic conditions, (ii) the extent and the persistence of the potential changes following a grassland/or cropland phase. We hypothesized that the level of microbial communities and enzyme activities could achieve a profile close to that of permanent grassland after the introduction of grassland for a few years in crop rotations. Soil biophysicochemical properties were studied. Our results indicated that the abundance of microbial communities and enzyme activities were positively correlated to soil C and N contents and negatively correlated to soil pH. The changes in microbial abundance level were strongly linked to the changes in functional level when grasslands are introduced into crop rotations. We also showed that a continuous crop regime had a stronger legacy on the soil biota and functions. By contrast, the legacy of a grassland regime changed quickly when the grassland regime is interrupted by recent culture events. A grassland regime enabled the restoration of functions after more than five cumulative years in the grassland regime.
Evaluation of the Efficacy of a Vaccination Program against Actinobacillus pleuropneumoniae Based on Lung-Scoring at Slaughter
Authors: Sipos Wolfgang , Cvjetković Vojislav , Dobrokes Branimir , Sipos Sabine
Published: September 2021

Porcine pleuropneumonia is of serious concern regarding lung health in pig production. Besides optimizing hygiene and pig management, specific vaccination against the causative agent, Actinobacillus pleuropneumoniae, is an important tool in the fight against this disease. As porcine pleuropneumonia may present with different clinical courses of disease, it is not always easy to objectively assess herd lung health state or to monitor improvements following specific therapeutic or prophylactic measures. Here, the effects of specific vaccination on lung health in a chronically diseased farrow-to-finish farm in Lower Austria experiencing an acute episode were monitored by means of an app-based electronic tool, enabling the scorers to document lung pathologies real-time at slaughter. At the time, when vaccination measures took effect, percentages of lungs affected by dorsocaudal pleurisy had decreased from 43 to 5 and the APP-index from 1.2 to 0.1, respectively. But not only pleurisies were diminished, also incidences and severities of bronchopneumonic alterations had dramatically decreased and exhibited interesting trends when set in connection to clinical signs. Overall, vaccination measures against Actinobacillus pleuropneumoniae proved to be very effective in restoring herd lung health.
Comprehensive Biological and Chemical Evaluation of Two Seseli Species (S. gummiferum and S. transcaucasicum)
Authors: Zengin Gokhan , Stojković Dejan , Mahomoodally Mohamad Fawzi , Jugreet Bibi Sharmeen , Paksoy Mehmet Yavuz , Ivanov Marija , Gašić Uroš , Gallo Monica , Montesano Domenico
Published: September 2021

Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl- and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.
Large-Scale Non-Targeted Metabolomics Reveals Antioxidant, Nutraceutical and Therapeutic Potentials of Sorghum
Authors: Ramalingam Ajay Prasanth , Mohanavel Williams , Premnath Ameena , Muthurajan Raveendran , Prasad P. V. Vara , Perumal Ramasamy
Published: September 2021

Sorghum is one of the most important food and feed cereal crops and has been gaining industrial importance in recent years for its biofuel, nutraceutical and antioxidant values. A genetic profile variation study was undertaken for the accumulation of phytochemicals in 61 diverse sorghum accessions differing in their growth habitat and grain color through non-targeted Gas Chromatography–Mass Spectrometry (GC-MS/MS) analysis. Mass Spectrometry–Data Independent AnaLysis (MS-DIAL) and MetaboAnalyst identified 221 metabolites belonging to 27 different phytochemicals. Tropical and temperate sorghums were distinct in their metabolic profiles with minimum overlaps, and 51 different metabolites were crucial in differentiating the two groups. Temperate sorghums had the ability to accumulate more of phenolic acids, phytosterols, flavonoids, carotenoids, and tropical sorghums for stress-related amino acids, sugars and fatty acids. Grain-color-based Partial Least Square–Discriminant Analysis (PLS-DA) analysis identified 94 Variable Importance in Projections (VIP) metabolites containing majority of flavonoids, phenylpropanoids and phytosterols. This study identified two sorghum lines (IS 7748 and IS 14861) with rich amounts of antioxidants (catechins and epicatechins) belonging to the group of condensed tannins that otherwise do not accumulate commonly in sorghum. Out of 13 metabolic pathways identified, flavonoid biosynthesis showed the highest expression. This study provided new opportunities for developing biofortified sorghum with enhanced nutraceutical and therapeutics through molecular breeding and metabolic engineering.
Enhancing Vibration Isolation Performance by Exploiting Novel Spring-Bar Mechanism
Authors: Shi Baiyang , Yang Jian , Li Tianyun
Published: September 2021

This study investigates the use of a spring-bar mechanism (SBM) in a vibration suppression system to improve its performance. The SBM, comprising bars and springs, is configured with a conventional linear spring-damper isolator unit. The dynamic response, force transmissibility, and vibration energy flow behaviour are studied to evaluate the vibration suppression performance of the integrated system. It is found that the SBM can introduce hardening, softening stiffness, or double-well potential characteristics to the system. By tuning the SBM parameters, constant negative stiffness is achieved so that the natural frequency of the overall system is reduced for enhanced low-frequency vibration isolation. It is also found that the proposed design yields a wider effective isolation range compared to the conventional spring-damper isolator and a previously proposed isolator with a negative stiffness mechanism. The frequency response relation of the force-excited system is derived using the averaging method and elliptical functions. It is also found that the system can exhibit chaotic motions, for which the associated time-averaged power is found to tend to an asymptotic value as the averaging time increases. It is shown that the time-averaged power flow variables can be used as uniform performance indices of nonlinear vibration isolators exhibiting periodic or chaotic motions. It is shown that the SBM can assist in reducing force transmission and input power, thereby expanding the frequency range of vibration attenuations.
Biodeterioration of Salón de Reinos, Museo Nacional del Prado, Madrid, Spain
Authors: Jurado Valme , Gonzalez-Pimentel José Luis , Hermosin Bernardo , Saiz-Jimenez Cesareo
Published: September 2021

The Salón de Reinos, a remnant of the 17th century Palacio del Buen Retiro, was built as a recreational residence under the reign of Felipe IV between 1632 and 1640 and was the main room for the monarch’s receptions. This Salón owes its name to the fact that the coats of arms (shields) of the 24 kingdoms that formed Spain in Felipe IV’s time were painted on the vault, above the windows. In addition, the ceiling shows an original decorative composition. The painted ceiling and window vaults showed deterioration evidenced by fissures, water filtration, detachments of the paint layer, and black stains denoting fungal colonization related to humidity. Ten strains of bacteria and 14 strains of fungi were isolated from the deteriorated paintings. Their biodeteriorative profiles were detected through plate assays. The most frequent metabolic functions were proteolytic and lipolytic activities. Other activities, such as the solubilization of gypsum and calcite and the production of acids, were infrequent among the isolates.
Compact and High Gain 4 × 4 Circularly Polarized Microstrip Patch Antenna Array for Next Generation Small Satellite
Authors: Elahi Manzoor , Trinh-Van Son , Yang Youngoo , Lee Kang-Yoon , Hwang Keum-Cheol
Published: September 2021

In this article, a high gain and compact 4 × 4 circularly polarized microstrip patch antenna array is reported for the data transmission of the next-generation small satellite. The radiating element of the circularly polarized antenna array is realized by the conventional model of the patch with truncated corners. A compact two-stage sequential rotational phase feeding is adopted that broadens the operating bandwidth of the 4 × 4 array. A small stub is embedded in the sequential rotational feed, which results in better performance in terms of the S-parameters and sequential phases at the output ports than sequential rotational feed without open stub. A prototype of the array is fabricated and measured. Fulfilling the application requirements of the next-generation small satellites, the array has the left-handed circularly polarized gain of more than 12 dBic with the axial ratio level below 1.5 dB in the ±10∘ angular space with respect to the broadside direction for the whole bandwidth from 8.05 GHz to 8.25 GHz. Moreover, the left-handed circularly polarized gain varies from 15 to 15.5 dBic in the desired band. The radiation patterns are measured; both the co- and X-pol are validated.
Fast Response GaAs Photodetector Based on Constructing Electron Transmission Channel
Authors: Guo Shuai , Chen Xue , Wang Dengkui , Fang Xuan , Fang Dan , Tang Jilong , Liao Lei , Wei Zhipeng
Published: September 2021

Low-dimensional GaAs photodetectors have drawn a great deal of attention because of their unique absorption properties and superior responsivity. However, their slow response speed caused by surface states presents challenges. In this paper, a mixed-dimensional GaAs photodetector is fabricated utilizing a single GaAs nanowire (NW) and a GaAs 2D non-layer sheet (2DNLS). The photodetector exhibits a fast response with a rise time of ~4.7 ms and decay time of ~6.1 ms. The high-speed performance is attributed to an electron transmission channel at the interface between the GaAs NW and GaAs 2DNLS. Furthermore, the fast electron channel is confirmed by eliminating interface states via wet passivation. This work puts forward an effective way to realize a high-speed photodetector by utilizing the surface states of low-dimensional materials.
A Detection Circuit for Improving the Unloading Transient Performance of the COT Controller
Authors: Zhang Xi , Wang Tianshi , Bao Bocheng
Published: September 2021

Fast load transient response and high light-load efficiency are two key features of the constant on-time (COT) control technique that has been widely used in numerous applications, such as for voltage regulators and point-of-load converters. However, when load step-down occurs during an on-time interval, the COT controller cannot respond until the COT interval expires. This delay causes an additional output voltage overshoot, resulting in unloading transient performance limitation. To eliminate the delay and improve the unloading transient response of the COT controller, a load step-down detection circuit is proposed based on capacitor current COT (CC-COT) control. In the detection circuit, the load step-down is monitored by comparing the measured capacitor current with the preset threshold voltage. Once the load step-down is monitored, the on-time is promptly truncated and the switch is turned off. With the proposed detection circuit, the CC-COT-controlled buck converter can monitor the load step-down without any delay and obtain less output voltage overshoot when the load step-down occurs during the on-time interval. PSIM circuit simulations are employed to demonstrate the feasibility of the detection circuit.
Distributed Robust Filtering over Sensor Networks with Quantized Measurement and Switching Topologies
Authors: Ding Shuchen , Zhu Fengzeng
Published: September 2021

This paper aims at exploring the theoretical research and distributed filtering design of state estimation for sensor networked systems with quantized measurement and switching topologies. In a sensor network, each sensor node has an independent static logarithmic quantizer function, and the quantized measurement is transmitted to the filtering network via the wireless network. In the corresponding filtering network, each local estimator achieves distributed consistent state estimation of the plant based on the local measurement and the neighboring shared information. In particular, the design of the distributed filter fully takes into account the fact that the communication links between the nodes are not fixed. That is, the communication topology has random switching, and such random switching behavior is described using Markov chains with partially unknown transition probabilities. A set of linear matrix inequalities gives the sufficient conditions for the existence of the distributed filter, while ensuring that the filter error system has the desired H∞ performance. Finally, two numerical simulations show the effectiveness of the design method.
Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins
Authors: Dridi Chaima , Millette Mathieu , Aguilar Blanca , Manus Johanne , Salmieri Stephane , Lacroix Monique
Published: September 2021

The aim of this study was to evaluate the effects of γ-irradiation (IR), ultrasound (US), and combined treatments of ultrasound followed by γ-irradiation (US-IR), ultrasound followed by enzymatic hydrolysis with and without centrifugation (US-E and US-EWC, respectively), and ultrasound followed by γ-irradiation and enzymatic hydrolysis (US-IRE), on the digestibility and the nutritional value of fermented beverages containing probiotics. Results showed that US (20 min), IR (3 kGy) and US-IR (tUS = 20 min, dose = 3 kGy) treatments raised protein solubility from 11.5 to 21.5, 24.3 and 29.9%, respectively. According to our results, these treatments were accompanied by the increased amount of total sulfhydryl groups, surface hydrophobicity and changes to the secondary structure of the proteins measured by Fourier-transform infrared spectroscopy (FTIR). Fermented probiotic beverages, non-enriched (C) and enriched with untreated (Cr) or treated cricket protein with combined treatments were also evaluated for their in vitro protein digestibility. Results showed that the soluble fraction of US-IRE fermented beverage had the highest digestibility (94%) as compared to the whole fermented tested beverages. The peptides profile demonstrated that US-IRE had a low proportion of high molecular weight (MW) peptides (0.7%) and the highest proportion of low MW peptides by over 80% as compared to the other treatments.
Effect of Korean Advanced Life Support Education on Non-Technical and Technical Skills of Nursing Students: A Pilot Study
Authors: Seo Yon Hee , Cho Kyong Ah
Published: September 2021

This study aimed to investigate the effect of the Korean Advanced Life Support (KALS) education program on the non-technical skills and technical skills of nursing students. This one-group pretest–posttest experimental study included 46 participants who were fourth year nursing students at Shinsung University located in Dangjin-si, Chungcheongnam-do Province, Republic of Korea. Data were collected in April 2021 and analyzed via SPSS/WIN 25.0, using a paired samples t-test. The current study results report a significant improvement in the non-technical skills from 30.58 to 47.16 points (t = −5.892, p < 0.001). Furthermore, KALS training improved communication confidence from 23.45 to 35.77 points (t = −6.563, p < 0.001), critical thinking tendency from 96.71 to 107.16 points (t = −3.352, p = 0.002), and self-efficacy in performing cardiopulmonary resuscitation from 33.56 to 49.81 points (t = −13.242, p < 0.001). Lastly, technical skills also improved from 18.35 to 27.94 points (t = −28.439, p < 0.001). Therefore, the findings indicate that KALS education was effective in improving the non-technical and technical skills of these nursing students. However, this study did not analyze the effect of the stress level experienced by the study participants in emergency situations on their non-technical and technical skill performance. Thus, future studies should verify the effect of external stressors, caused by unpredictable emergencies, on non-technical and technical skill performance.
Usefulness of Colon Assessment by Magnetic Resonance Enterography in Pediatric Patients with Inflammatory Bowel Disease—Retrospective Case Series
Authors: Sieczkowska-Golub Joanna , Marcinska Beata , Dadalski Maciej , Jarzebicka Dorota , Jurkiewicz Elzbieta , Kierkus Jaroslaw
Published: September 2021

Background: Magnetic resonance enterography (MRE) is an excellent way to study the small bowels. During such an examination, the colon is also seen within the field of study. The aim of this study was to evaluate the effectiveness of MRE in detecting characteristics of active inflammatory bowel disease (IBD) in the colon, in comparison to different features seen in colonoscopies. Methods: This retrospective study was conducted with 41 children. Features of active inflammation we considered were wall thickening; contrast enhancement; incorrect signal in the DWI sequence in the MRE; and presence of ulceration, erosion, erythema, spontaneous bleeding and a decrease of the vascular pattern seen in colonoscopy. The colon was divided into six segments: caecum, ascending, transverse, descending, sigmoid and rectum. Results: The sensitivity of MRE was, on average, 50–75%, and as high as 92–100%, depending on the segment. The most important feature for which there was the most dependencies was ulceration. In the analysis of intestinal wall thickness, the AUC value >0.8 was detected as ulceration (segments: cecum, ascending, descending colon, sigmoid), spontaneous bleeding (ascending colon and sigmoid) and decreased vascular pattern (ascending, transverse, descending colon). Conclusions: Evaluation of qualitative structural changes in MRE distinguishes patients with inflammation in colonoscopy from patients without lesions, with high diagnostic accuracy, albeit higher specificity than sensitivity.
Severe Fatigue and Memory Impairment Are Associated with Lower Serum Level of Anti-SARS-CoV-2 Antibodies in Patients with Post-COVID Symptoms
Authors: Molnar Tihamer , Varnai Reka , Schranz Daniel , Zavori Laszlo , Peterfi Zoltan , Sipos David , Tőkés-Füzesi Margit , Illes Zsolt , Buki Andras , Csecsei Peter
Published: September 2021

Background: Post-COVID manifestation is defined as persistent symptoms or long-term complications beyond 4 weeks from disease onset. Fatigue and memory impairment are common post-COVID symptoms. We aimed to explore associations between the timeline and severity of post-COVID fatigue and anti-SARS-CoV-2 antibodies. Methods: Fatigue and memory impairment were assessed in a total of 101 post-COVID subjects using the Chalder fatigue scale (CFQ-11) and a visual analogue scale. Using the bimodal scoring system generated from CFQ-11, a score ≥4 was defined as severe fatigue. Serum anti-SARS-CoV-2 spike (anti-S-Ig) and nucleocapsid (anti-NC-Ig) antibodies were examined at two time points: 4–12 weeks after onset of symptoms, and beyond 12 weeks. Results: The serum level of anti-S-Ig was significantly higher in patients with non-severe fatigue compared to those with severe fatigue at 4–12 weeks (p = 0.006) and beyond 12 weeks (p = 0.016). The serum level of anti-NC-Ig remained high in patients with non-severe fatigue at both time points. In contrast, anti-NC-Ig decreased significantly in severe fatigue cases regardless of the elapsed time (4–12 weeks: p = 0.024; beyond 12 weeks: p = 0.005). The incidence of memory impairment was significantly correlated with lower anti-S-Ig levels (−0.359, p < 0.001). Conclusion: The systemic immune response reflected by antibodies to SARS-CoV-2 is strongly correlated with the severity of post-COVID fatigue.
Mapping of Chronic Pulmonary Aspergillosis in Africa
Authors: Olum Ronald , Osaigbovo Iriagbonse Iyabo , Baluku Joseph Baruch , Stemler Jannik , Kwizera Richard , Bongomin Felix
Published: September 2021

Africa has a high burden of tuberculosis, which is the most important risk factor for chronic pulmonary aspergillosis (CPA). Our goal was to systematically evaluate the burden of CPA in Africa and map it by country. We conducted an extensive literature search for publications on CPA in Africa using the online databases. We reviewed a total of 41 studies published between 1976 and 2021, including a total of 1247 CPA cases from 14 African countries. Most of the cases came from Morocco (n = 764, 62.3%), followed by South Africa (n = 122, 9.9%) and Senegal (n = 99, 8.1%). Seventeen (41.5%) studies were retrospective, 12 (29.3%) were case reports, 5 case series (12.2%), 5 prospective cohorts, and 2 cross-sectional studies. The majority of the cases (67.1%, n = 645) were diagnosed in men, with a median age of 41 years (interquartile range: 36–45). Active/previously treated pulmonary tuberculosis (n = 764, 61.3%), human immunodeficiency virus infection (n = 29, 2.3%), diabetes mellitus (n = 19, 1.5%), and chronic obstructive pulmonary disease (n = 10, 0.8%) were the common co-morbidities. Haemoptysis was the most frequent presenting symptom, reported in up to 717 (57%) cases. Smoking (n = 69, 5.5%), recurrent lung infections (n = 41, 3%) and bronchorrhea (n = 33, 3%) were noted. This study confirms that CPA is common in Africa, with pulmonary tuberculosis being the most important risk factor.
Importance of Cry Proteins in Biotechnology: Initially a Bioinsecticide, Now a Vaccine Adjuvant
Authors: Gonzalez-Vazquez Maria Cristina , Vela-Sanchez Ruth Abril , Rojas-Ruiz Norma Elena , Carabarin-Lima Alejandro
Published: September 2021

A hallmark of Bacillus thuringiensis bacteria is the formation of one or more parasporal crystal (Cry) proteins during sporulation. The toxicity of these proteins is highly specific to insect larvae, exerting lethal effects in different insect species but not in humans or other mammals. The aim of this review is to summarize previous findings on Bacillus thuringiensis, including the characteristics of the bacterium, its subsequent contribution to biotechnology as a bioinsecticide due to the presence of Cry proteins, and its potential application as an adjuvant. In several studies, Cry proteins have been administered together with specific antigens to immunize experimental animal models. The results have shown that these proteins can enhance immunogenicity by generating an adequate immune response capable of protecting the model against an experimental infectious challenge, whereas protection is decreased when the specific antigen is administered without the Cry protein. Therefore, based on previous results and the structural homology between Cry proteins, these molecules have arisen as potential adjuvants in the development of vaccines for both animals and humans. Finally, a model of the interaction of Cry proteins with different components of the immune response is proposed.
Telocytes and Lymphatics of the Human Colon
Authors: Zurzu Mihai , Nicolescu Mihnea Ioan , Mogoantă Laurențiu , Pantea Stelian , Rusu Mugurel Constantin
Published: September 2021

Background: Telocytes (TCs) are a peculiar morphological type of stromal cells. They project long and moniliform telopodes, visible on various bidimensional sections. Originally regarded as “interstitial Cajal-like cells”, gastrointestinal TCs were CD34+. Further double-labelling studies found that colon TCs are negative for the expressions of the PDGFR-α and α-SMA. However, the TCs in colon were not distinguished specifically from endothelial cells (ECs), vascular or lymphatic. A combinational approach is important for accurate TC identification. Hence, we designed an immunohistochemical study of human colon to check whether ECs and CD34+ TCs express different markers. Methods: Immunohistochemistry was performed on archived paraffin-embedded samples of human colon (nine cases) for the following markers: CD31, CD34, CD117/c-kit and D2-40 (podoplanin). Results: A distinctive population of CD34+ TCs was found coating the myenteric ganglia. However, also perivascular cells and vascular ECs were CD34+. c-kit expression was equally found in interstitial Cajal cells (ICCs) and perivascular cells. The CD34 TCs did not express c-kit. As they were equally CD31- and D2-40- they were assessed as different from ECs. Conclusions: Testing specific markers of ECs, vascular and lymphatic, in the same tissues in which CD34+ TCs are found, is much more relevant than to identify TCs by transmission electron microscopy alone.
Epidemiological Characterization of Clinical Fungal Isolates from Pauls Stradinš Clinical University Hospital, Latvia: A 4-Year Surveillance Report
Authors: Jain Nityanand , Jansone Inese , Obidenova Tatjana , Sīmanis Raimonds , Meisters Jānis , Straupmane Dagnija , Reinis Aigars
Published: September 2021

Nosocomial fungal infections are an emerging global public health threat that requires urgent attention and proper management. With the limited availability of treatment options, it has become necessary to understand the emerging epidemiological trends, mechanisms, and risk factors. However, very limited surveillance reports are available in the Latvian and broader European context. We therefore conducted a retrospective analysis of laboratory data (2017–2020) from Pauls Stradinš Clinical University Hospital (PSCUH), Riga, Latvia, which is one of the largest public multispecialty hospitals in Latvia. A total of 2278 fungal isolates were analyzed during the study period, with Candida spp. comprising 95% of the isolates, followed by Aspergillus spp. and Geotrichum spp. Amongst the Candida spp., C. albicans and C. glabrata made up about 75% of the isolates. The Department of Lung Diseases and Thoracic Surgery had the highest caseload followed by Intensive Care Department. Majority of the fungal isolates were collected from the bronchoalveolar lavage (37%), followed by urine (19%) and sputum (18%) samples. A total of 34 cases of candidemia were noted during the study period with C. albicans being the most common candidemia pathogen. Proper surveillance of emerging epidemiological trends serve as the most reliable and powerful cornerstone towards tackling this emerging threat.
Study on Enhancing the Thermoelectric Properties of Ti2CrSn Alloys
Authors: Yu Guangfa , Song Shihao , Ren Yanwei , Guo Jia , Yan Liqin , Lu Zunming , Shen Jun
Published: September 2021

Based on the Hg2CuTi structure, the full-Heusler alloy Ti2CrSn, with a ground state band gap of semiconductor, is a thermoelectric material with potential applications. Through preparing Ti2CrSn1−xAlx (x = 0, 0.05, 0.1, 0.15, 0.2) series bulk materials via arc melting, the effects of the electrical and thermal transport properties of Ti2CrSn series alloys were investigated, and different Al doping on the phase structure, the microscopic morphology, and the thermoelectric properties of Ti2CrSn were examined. The results show that the materials all exhibit characteristics of p-type semiconductors at the temperature range of 323 to 923 K. Al elemental doping can significantly increase the Seebeck coefficient and reduce the thermal conductivity of the materials. Among them, the sample Ti2CrSn0.8Al0.2 obtained a maximum value of 5.03 × 10−3 for the thermoelectric optimal ZT value at 723 K, which is 3.6 times higher than that of Ti2CrSn.
Fail-Safe Joints between Copper Alloy (C18150) and Nickel-Based Superalloy (GH4169) Made by Transient Liquid Phase (TLP) Bonding and Using Boron-Nickel (BNi-2) Interlayer
Authors: Zhang Chengcong , Shirzadi Amir
Published: September 2021

Joining heat conducting alloys, such as copper and its alloys, to heat resistant nickel-based superalloys has vast applications in nuclear power plants (including future fusion reactors) and liquid propellant launch vehicles. On the other hand, fusion welding of most dissimilar alloys tends to be unsuccessful due to incompatibilities in their physical properties and melting points. Therefore, solid-state processes, such as diffusion bonding, explosive welding, and friction welding, are considered and commercially used to join various families of dissimilar materials. However, the solid-state diffusion bonding of copper alloys normally results in a substantial deformation of the alloy under the applied bonding load. Therefore, transient liquid phase (TLP) bonding, which requires minimal bonding pressure, was considered to join copper alloy (C18150) to a nickel-based superalloy (GH4169) in this work. BNi-2 foil was used as an interlayer, and the optimum bonding time (keeping the bonding temperature constant as 1030 °C) was determined based on microstructural examinations by optical microscopy (OM), scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), tensile testing, and nano-hardness measurements. TLP bonding at 1030 °C for 90 min resulted in isothermal solidification, hence obtained joints free from eutectic phases. All of the tensile-tested samples failed within the copper alloy and away from their joints. The hardness distribution across the bond zone was also studied.
Biofilm Formation Ability of Arcobacter-like and Campylobacter Strains under Different Conditions and on Food Processing Materials
Authors: Šilha David , Sirotková Sabina , Švarcová Karolína , Hofmeisterová Leona , Koryčanová Květa , Šilhová Lucie
Published: September 2021

Campylobacter jejuni is the most frequent cause of bacterial gastrointestinal food-borne infection worldwide. The transmission of Campylobacter and Arcobacter-like species is often made possible by their ability to adhere to various abiotic surfaces. This study is focused on monitoring the biofilm ability of 69 strains of Campylobacter spp. and lesser described species of the Arcobacteraceae family isolated from food, water, and clinical samples within the Czech Republic. Biofilm formation was monitored and evaluated under an aerobic/microaerophilic atmosphere after cultivation for 24 or 72 h depending on the surface material. An overall higher adhesion ability was observed in arcobacters. A chi-squared test showed no association between the origin of the strains and biofilm activity (p > 0.05). Arcobacter-like species are able to form biofilms under microaerophilic and aerobic conditions; however, they prefer microaerophilic environments. Biofilm formation has already been demonstrated at refrigerator temperatures (5 °C). Arcobacters also showed higher biofilm formation ability at the temperature of 30 °C. This is in contrast to Campylobacter jejuni NP 2896, which showed higher biofilm formation ability at temperatures of 5–30 °C. Overall, the results demonstrated the biofilm formation ability of many strains, which poses a considerable risk to the food industry, medical practice, and human health.