bluuebunny/crossref_metadata_2025 huggingface dataset:
El Secreto de Andrómaca: La esclavitud de las labores domésticas en "La Condición Humana" de Hannah Arendt
Authors: Galindo Fernando , Hurtado Rafael
Published: August 2020

La nota distintiva más noble del pensamiento de Hannah Arendt es su inequívoca apuesta por la libertad. Su amor por este concepto la llevó a considerar las labores domésticas como actividades que limitan el desarrollo humano político. El texto analiza el concepto de labor y lo vincula con la noción de necesidad en el griego clásico. En esa línea, se ofrece una apología del concepto de “labor”, desde de una interpretación relativa a un pasaje clave de la Iliada, citado por Arendt misma para criticar la necesidad, un pasaje que interpretado desde nuestra óptica nos parece que permite una mejor comprensión de la labor, las labores domésticas y la relevancia del hogar.
Isolation, screening antimicrobial activity and identification of fungi from marine sediments of the area Thanh Lan, Co To, Vietnam
Authors: Hong Minh Le Thi , Mai Anh Nguyen , Thi Quyen Vu , Thu Huyen Vu Thi , Mai Huong Doan Thi , Van Cuong Pham , Van Minh Chau
Published: August 2020

Marine environment is rich in natural product resources, including marine microorganisms, especially fungi which are not only seen as a potential source of highly applicable bioactive substances but also can provide for science new chemical structures. The objective of this study is to isolate and screen fungal strains with antibacterial activity from the marine environment. Twenty five strains of fungi were isolated from marine sediments of Thanh Lan, Co To island and assessed on antibiotic activity against 7 tested microbial strains, including three Gram-negative bacteria (Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Salmonella enterica ATCC13076), three Gram-positive bacteria (Enterococcus faecalis ATCC29212, Stapphylococus aureus ATCC25923, Bacillus cereus ATCC 13245), and the yeast Candida albicans ATCC10231. The minimum inhibitory concentration (MIC) against the tested microorganisms was determined for the crude extracts obtained from the culture broths after ethyl acetate extraction and vacuum rotary evaporation. Three strains with the highest antimicrobial activity M26, M30 and M45 were capable of inhibiting 4 - 5 of the 7 tested microorganisms with MIC values from 64 to 256 μg/ml, depending on each tested strain. Morphological and phylogenetic investigations based on 18S rRNA gene sequences of the three selected strains showed that strains M26 and M30 belonged to the genus Penicillium, whereas strain M45 belonged to the genus Neurospora. The sequences of 18S rRNA gene of three strains M26, M30 and M45 were registered on GenBank database with accession numbers: MH673730, MH673731, MH673732, respectively. Research results showed that marine environment has a great potential in isolation of fungal strains for the search for antibacterial substances as well as other biologically active compounds.
Drought stress - related functional characterization of transcription factor GmNAC085 in soybean
Authors: Khanh Hoa Tran Thi , Ngoc Tuyet Huynh , Phuong Thao Nguyen , Lan Xuan Hoang Thi
Published: August 2020

Studies on soybean GmNAC085 transcription factor revealed that the gene expression in plants was induced by water shortage treatments and its overexpression in the model plant Arabidopsis displayed improved plant tolerance characteristics towards drought stress. In this study, we continued analyzing the biological functions of GmNAC085 using transgenic soybean system overexpressing GmNAC085 gene, by targeting at a number of plant physiological features and biochemical activities in response to limited water growing condition. Compared to the wild-type, the transgenic line demonstrated that it possessed stress tolerance characters, including enhanced elongation of taproot, minimized reduction of shoot growth, lower intracellular H2O2 content and stronger peroxidase enzyme activity under drought condition. The results of this study therefore suggest the transgenic plants had better drought tolerance and the GmNAC085 plays important role in aiding plants to cope with water deficit condition, probably via regulating the growth of roots and shoots, and activities of reactive-oxygen-species- scavenging enzymes.
Detection of 16S rRNA and 23S rRNA gene mutations in multidrug resistant Salmonella serovars isolated from different sources using RNA sequencing method
Authors: Thanh Viet Nguyen , Bich Thuy Vo Thi
Published: August 2020

The rapid emergence of resistant bacteria is occurring worldwide. Antibiotic resistance is a serious problem for human beings because pathogenic microorganisms that acquire such resistance void antibiotic treatments. Bacterial antibiotic resistance mechanisms include efflux, reduced influx, modification and degradation of the drug, as well as mutation, modification or overexpression of the target. However, our knowledge as to how bacteria acquire antibiotic resistance is still fragmented, especially for ribosome-targeting drugs. Salmonella is a leading cause of foodborne salmonellosis in the world. The number of antibiotic resistant isolates identified in humans is steadily increasing, suggesting that the spread of antibiotic resistant strains is a major threat to public health. Salmonella is commonly identified in a wide range of animal hosts, food sources, and environments, but our knowledge as to how Salmonella resistance to antibiotics is still fragmented in this ecologically complex serovar. Therefore, the aim of this study was to support for finding novel mechanisms that render bacteria resistant to the ribosome targeting antibiotics, we screen for antibiotic resistant 16S and 23S ribosomal RNAs (rRNAs) in multidrug resistant Salmonella serovars isolated from raw retail meats isolated from Hanoi, Vietnam. Bioinformatic analysis identified 193 unknown novel mutations (64 mutations in 16S rRNA and 129 mutations in 23S rRNA genes). These mutations might play a role in streptomycin resistant in Salmonella serovars. These results suggest that uncharacterized antibiotic resistance mutations still exist, even for traditional antibiotics. This study is only a preliminary kind, further validation before they are applied in Salmonella or other closely related species are required.
Effect of immunocytotherapy on the state of the immune system of women with idiopathic habitual miscarriage
Authors: Krechetova L. V. , Vtorushina V. V. , Inviyaeva E. V. , Vanko L. V. , Nikolaeva M. A. , Tetruashvili N. K.
Published: August 2020

We aimed  for assessing effects of immunocytotherapy upon  the subpopulations of CD4+CD25highFoxP3+cellswithnaturalregulatoryactivityandactivatedTh17cellswiththeCD4+CD25highRORγt+phenotype, as well as in vitro production of cytokines in mitogen-stimulated cells from  peripheral blood  in the patients with idiopathic habitual miscarriage (IHM). The study group consisted of 33 patients with IHM who became pregnant after a pre-gestational alloimmunization. In 27 patients, the pregnancy was prolonged to the  full term  and  ended  with the  birth  of viable babies,  in six cases it was terminated before  12 weeks of gestation. Before  administration of immunocytotherapy (ICT), 19 patients were examined, of them  16 after alloimmunization outside of pregnancy, 17 at 5-6 and 8-9 weeks of pregnancy. Eleven patients were immunized at 12 weeks of pregnancy. In the control group,  12 fertile women  outside  pregnancy and 10 women  at 12 weeks of physiological pregnancy were examined. The proportion of FoxP3+and RORγt+cells with the CD4+CD25highphenotype was evaluated among  T-lymphocytes from peripheral blood,  as well as content of proinflammatory cytokines (IFNγ,  TNFα, IL-1β, IL-2, IL-5, IL  -6,  IL-8, IL-12p70) and  anti-inflammatory factors  (IL-4, IL-10), as well as IL-17  amounts.

We have found  that,  following pre-gestational alloimmunization, the women  who lost this pregnancy, had a  low  level  of  FoxP3+Тregs that  suppress  pro-inflammatory Th17-dependent  reactions, however, without changing levels of activated Th17  cells (CD4+CD25highRORγt+   lymphocytes). These  facts,  along  with  high in vitro production of IL-17  by peripheral blood cells at the terms of 5-6 weeks of gestation, suggest that,  after pre-gestational alloimmunization in women  with miscarriage, a predilection is formed  to pro-inflammatory cytokine production. However, at the 5-6 week-period, it is realized  not in the Th1 direction of, but towards Th17 response, and a low level of CD4+CD25highRORγt+cells may reflect an increased migration of Th17 cells from peripheral blood to the uterine endometrium.

Thus,  we have shown  the  effect of immunocytotherapy upon  subpopulational composition of peripheral blood  lymphocytes and  the  cytokine profile,  as well as upon  the  course  of first trimester and  outcomes of pregnancy in women  with idiopathic habitual miscarriage.
Advancing diagnostics of chronic paradontitis in children
Authors: Agarkov N. M. , Makaryan A. S. , Gontareva I. S.
Published: August 2020

Chronic periodontitis in children and adolescents holds a lead place in morbidity pattern of dental pathology. Development of chronic periodontitis is accompanied by emergence of various complications in the maxillofacial region, leading to bite disturbance being of high relevance for pediatric patients. These and other complications are related to immune system immaturity in children and adolescents as well as virulence of microorganisms. However, the immunological changes developing in children with chronic periodontitis remain poorly studied. The aim of the work was to improve diagnostics of chronic periodontitis in children and adolescents based on informative parameters of systemic immunity and discriminative models taking into account such changes. We examined systemic immunity in 127 children and adolescents with chronic periodontitis, aged 12 to 16 years, by using flow cytometry and enzyme immunoassay. In control group, age-matched 108 patients lacking overt somatic and dental pathology were enrolled. Generation of mathematical models was carried out by using a discriminative approach, whereas informativeness was assessed in accordance with generally accepted formula. Relative and absolute count of peripheral blood CD13+ cells exert the peak informativeness holding the first and second ranking places with marked dominance of informativeness value for assessing relative amount of CD13+ cells are among immunological parameters in children with chronic periodontitis. High informativeness value evidencing about pronounced intensity of developed pathological changes and diagnostic significance for chronic periodontitis in children is intrinsic to the relative percentage of peripheral blood CD8+ cells being slightly lower than that one in absolute count of CD13+ cells. On the other hand, humoral immune-parameters were of lowest informative value among all analyzed immunological parameters in patients with chronic periodontitis serum referring to all antibody classes. Generated discriminative models for the most valuable immunological parameters ensure adequate medical diagnostics for chronic periodontitis in childhood. Diagnostic sensitivity for created mathematical models was high and reached 0.94, whereas diagnostic specificity — 0.92. Immunological examination of patients improves diagnostics of chronic periodontitis. It was found that patients with chronic periodontitis had lowered immune status peaking in decreased absolute and relative count of peripheral blood CD3+ lymphocytes. Finally, parameters of humoral immunity in children with chronic periodontitis were also reduced.
Detection of international high-risk clones of food-borne pathogens Salmonella and Escherichia coli in the Russian Federation
Authors: Kaftyreva L. A. , Egorova S. A. , Makarova M. A.
Published: August 2020

The use of whole genome sequencing, standard analyze methods and international databases allows to detect international high-risk clones of food-borne pathogens, to assess their evolution and geographical distribution due to international trade of animal food products and farm animals. In Saint-Petersburg, Russian Federation, Salmonella Newport, Salmonella Kentucky and Escherichia coli O26:H11 of well-known international high-risk clones were first identified from patients with diarrhea. Two S. Kentucky strains isolated in 2015 and 2019 were identical to the international clone ST198 widely distributed in European countries: had multidrug resistance, high-level fluoroquinolone resistance (MIC of ciprofloxacin    32,0 mg/l) due to three single-nucleotide substitutions in the gyrA (Ser83Phe and Asp87Asn) and parC (Ser80Ile). In 2008, a multidrug resistant S. Newport strain resistant to extended-spectrum cephalosporins due to AmpC-cephalosporinase CMY-2 was isolated, belonging to the international clone of S. Newport MDR-AmpC/CMY-2  which caused sporadic cases and outbreaks in the USA and Europe in the 2000s. The plasmid containing the blaCMY-2 had a PstI-restriction profile, one of the most prevalent in the international clone. E. coli O26:H11 strains, isolated in Saint-Petersburg,  produced the shiga-like toxin STX1 (stx1a), had additional virulence genes: ehxA (enterohemolysin), katP (catalase peroxidase), espP (serine protease), as well as cba (colicin B), gad (glutamate decarboxylase), cif (type III secreted effector), iss (increased serum survival), belonged to the phylogenetic group B1 and the international high-risk clone  E. coli O26:H11 ST21 widely distributed in Europe and the USA. 25% of the strains had multidrug resistance and produced CTX-M extended spectrum beta-lactamases. In the Russian Federation E. coli O26 is consider as the pathogen of diarrheal diseases and registered as Enteropathogenic E. coli in routine bacteriological laboratories without detecting of H-antigen and shiga-like toxins.
Lung memory T-cell response in mice following intranasal immunization with influenza vector expressing mycobacterial proteins
Authors: Shurygina A.-P. S. , Zabolotnykh N. V. , Vinogradova T. I. , Vasilyev K. A. , Buzitskaya Zh. V. , Stukova M. A.
Published: August 2020

Improving specific prevention of tuberculosis continues to be a top priority in phthisiology. “Prime-boost” vaccination schemes aim to maintain adequate levels of specific immunity while forming long-term protection. They are based on sequential use of BCG vaccine and new vaccine candidates expressing protective mycobacterial proteins. The development of new tuberculosis prevention approaches requires an understanding of how the anti-tuberculosis immune response forms and which mechanisms provide TB protection. Since tuberculosis is an airborne infection, vaccine effectiveness largely depends on mucosal immunity based on the formation of long-lived, functionally-active memory T-lymphocytes in the respiratory tract. We have previously shown that the influenza vector expressing ESAT-6 and Ag85A mycobacterial proteins (Flu/ESAT-6_Ag85A) in vaccination scheme of intranasal boost immunization resulted in significant increase of BCG's protective effect according to key indicators aggregate data in experimental tuberculosis infection. The aim of this work was to study the effect of intranasal immunization with the Flu/ESAT-6_Ag85A influenza vector on the formation of antigen-specific central and effector memory T cells and the cytokine-producing activity of effector T cells (TEM) in BCG standard and “BCG prime — influenza vector boost” vaccination schemes in mice. Intranasal immunization with the influenza vector has been shown to increase the proportion of antigen-specific CD4+ central memory T cells (TCM) in the pool of activated lymphocytes of lung and spleen reaching significant differences from the BCG group in the percentage of spleen CD4+ TCM (p 0.01). In contrast to BCG, vaccination with the studied vaccine candidate was accompanied by accumulation of highly differentiated CD8 effector cells in lung, the target organ during tuberculosis infection. Comparative evaluation of the cell-mediated, post-vaccine immune response after immunization with influenzavector-based vaccine candidate (intranasal/mucosal) or BCG vaccine (subcutaneous) showed advantages in the mucosal group: in formation of functionally active subpopulations of effector CD4 and CD8 T lymphocytes (CD44highCD62Llow) in lungs secreting IL-2 as well as polyfunctional cells capable of coproducing two cytokines (IFNγ/TNFα or IFNγ/IL-2) or three cytokines (IFNγ/TNFα/IL-2). Due to their more pronounced effector function, polyfunctional T-lymphocytes can be considered to be potential immunological markers of protective immunity in tuberculosis.
Risk factors for constant glycemic variability in pregnant women: a case-control study
Authors: Barros Grasiela Martins , Figueiredo Lyvia da Silva , Souza Priscilla Alfradique de , Souza Beatriz Paiva e Silva de , Ferreira Helen Campos , Cavalcanti Ana Carla Dantas
Published: August 2020

ABSTRACT Objective: to identify the factors associated with pregnancy that influence constant glycemic variability. Method: a case-control study with random sampling. The medical records of 417 pregnant women were divided into case group (200 pregnant women with constant glycemic variability) and control group (217 pregnant women without constant glycemic variability). Data were collected from 2009 to 2015. Results: pregnant women aged 25 years and over, with family history of diabetes mellitus, with systemic arterial hypertension, overweightness or obesity, sedentarism and polycystic ovarian syndrome are more likely to present changes in blood glucose. Conclusion: The study demonstrated that risk factors associated with pregnancy increase the risk of constant glycemic variability. The findings will allow reassessment of the interventions during pregnancy, providing an increase in nursing care quality.
Evidence of nursing patterns of knowing communicated by the brazilian press before Florence Nightingale’s model
Authors: Brandão Ana Paula da Costa Lacerda , Peres Maria Angélica de Almeida , Aperibense Pacita Geovana Gama de Sousa , Lopes Rafael Oliveira Pitta , Santos Jéssica de Castro , Brandão Marcos Antônio Gomes
Published: August 2020

ABSTRACT Objective: to identify evidence of nursing patterns of knowing disseminated by the Brazilian press before the implementation of Florence Nightingale’s model in Brazil and categorize topics of journalistic articles according to Carper’s and White’s patterns of knowing. Methods: categorical content analysis of materials related to Florence Nightingale, published in Brazil between 1850 and 1919, collected at Hemeroteca Digital. Four analysts identified themes of journalistic article, performing classification in patterns of knowing. Results: there was a predominance of evidence of the sociopolitical pattern followed by the empirical pattern. In the analyses per decade, ethical and aesthetic patterns showed predominance between 1860 and 1870, respectively. Conclusion: White’s classification by nursing patterns of knowing was useful in understanding precursor themes of professional/disciplinary knowledge that spread in Brazil, linked to Nightingale’s character, in addition to the repercussions of her actions and her expanded sociopolitical perspective.
UM ESTUDO SOBRE AS AÇÕES DOCENTES EM UMA ESCOLA DE 1º CICLO EM PORTUGAL
Authors: PIRATELO MARCUS VINICIUS MARTINEZ , ARRUDA SERGIO DE MELLO , COSTA NILZA MARIA VILHENA NUNES DA , PASSOS MARINEZ MENEGHELLO
Published: August 2020

RESUMO: Este artigo apresenta resultados de um estudo realizado a respeito da ação docente de dois professores em uma escola de 1º ciclo em Portugal. Os fundamentos metodológicos que conduzem a coleta e a organização dos dados baseiam-se nos procedimentos indicados pela Análise Textual Discursiva (ATD) de Moraes e Galiazzi (2011). As aulas dos professores foram analisadas utilizando-se de uma forma de organização dos dados provenientes de dois instrumentos analíticos distintos: a Matriz do Professor, de Arruda e Passos (2017), e o Quadro intitulado “os objetivos e motivos da ação”, de Tardif e Lessard (2008). Foi possível identificar as categorias de ação docente realizadas pelos professores e a frequência dessas categorias de ação. Por meio de uma nova maneira de organizar os dados foi possível concluir que o ambiente de sala de aula e o estilo pedagógico de cada professor podem ser fatores decisivos para as ações desempenhadas em classe.
Exchange, Friendship and Regional Relations in the Upper Xingu
Authors: Novo Marina Pereira , Guerreiro Antonio
Published: August 2020

Abstract The aim of this work is to describe and analyze the different types of relationships established between the peoples of the Upper Xingu through their regional rituals. Starting from a description of the uluki exchange ceremony, we will discuss how this ritual, by mobilizing Xinguano ideas about friendship, produces contexts of interaction in which characteristics of intra-village sociality are extended to regional sociality. At the same time that the uluki defines, together with other regional rituals, a certain “Xinguano interiority” (the world of multi-community rituals), it is also one of its main forms of opening, having the potential to attract to the Xinguano world not only singular persons, but entire groups.
Pregnancy and dialysis
Authors: Ribeiro Catarina Isabel , Silva Natália
Published: August 2020

ABSTRACT The pregnancy rate of women on dialysis is still very low when compared to that of the remaining population. However, recent years have seen an increase in the success rates of these pregnancies. Among the main precautions that must be taken with pregnant women on dialysis are the maintenance of low levels of pre-dialysis urea, the adequacy of the tension profile, the control of anemia and care to avoid infections, nutritional deficits, changes in phosphorus-calcium metabolism and electrolytic fluctuations. It is also necessary to strictly monitor fetal growth and development. Pregnant women on dialysis have a higher probability of maternal and fetal complications; thus the importance of a multidisciplinary approach among nephrologists, obstetricians and pediatricians. The main objective of this study was to review the literature evidence available on pregnancy on dialysis, on the basic principles of the pathophysiology of pregnant women and their particularities in kidney disease. We will address available treatment options, benefits and risks, anticipating possible future challenges. At the end, we will present a clinical case to illustrate the topic.
Medidas de prevenção à Covid-19 no retorno às aulas: Protocolos de 13 países
Authors: Soares Letícia , Schoen Teresa Helena
Published: August 2020

As políticas de isolamento social respondentes à pandemia do SARS-CoV-2 ocasionaram o fechamento de escolas em mais de 190 países. As escolas estão organizando sua estrutura e funcionamento para que a reabertura se dê da forma mais segura possível. O presente artigo teve por objetivo arrolar as medidas de biossegurança adotadas por outros países no retorno às aulas de alunos na faixa etária dos 10 aos 19 anos. Analisou-se protocolos para reabertura das instituições educacionais de 13 países. Foram identificadas providências em relação a quem pode frequentar a escola, higiene pessoal e limpeza da escola, uso de máscaras e distanciamento social. A reabertura das escolas deve levar em conta a situação epidemiológica de cada região e as condições de infraestrutura de cada instituição.
Study on the phase behaviour of condensate gas in nanostructure media
Authors: Ju-hua Li , Ao Wang , Bin Zheng
Published: August 2020

Condensate dropout near the wellbore region in gas condensate reservoirs is the main reason for low well deliverability. Here, the gas and condensate phase behaviour in this region was investigated to find the conditions for better deliverability. Firstly, micro-computerised tomography (CT) experiments were carried out on a condensate gas system in four sandstone rock core samples. However, due to limitations of the experimental technique, the phase characteristics of the condensate gas could not be fully described in nanostructure pore scale. Therefore, an improved Peng–Robinson equation of state incorporating capillary force was used to simulate the flash phase behaviour of condensate gas and the constant volume depletion experiment in a single pore. Then, based on a pore network model generated using a random fractal method, a modified model was developed to investigate fluid distribution in a gas condensate system at the nanostructure pore scale. A new method was developed that applies a flash calculation in all throats in the network to estimate the amount of accumulated condensate in the corners of the throats. Finally, the phase behaviour of condensate gas in different types of porous media was studied in parallel capillary bundle and three-dimensional network models. The experimental phenomena observed in the CT experiments were explained by the characteristic simulation study. The results of this study provide theoretical support for phase behaviour predictions in condensate gas reservoirs.
Market Power, Inequality, and Financial Instability
Authors: Cairó Isabel , Sim Jae
Published: August 2020

Over the last four decades, the U.S. economy has experienced a few secular trends, each of which may be considered undesirable in some aspects: declining labor share; rising profit share; rising income and wealth inequalities; and rising household sector leverage and associated financial instability. We develop a real business cycle model and show that the rise of market power of the firms in both product and labor markets over the last four decades can generate all of these secular trends. We derive macroprudential policy implications for financial stability.
The Multiplier Effect of Education Expenditure
Authors: De Ridder Maarten , Hannon Simona M. , Pfajfar Damjan
Published: August 2020

This paper examines the short-run effects of federal education expenditures on local income. We exploit city-level variation in exposure to national changes in the $30-billion Federal Pell Grant Program, which is the largest program to help low-income students attend college in the U.S., to calculate fiscal multipliers of education expenditures. An increase in Pell grants by 1 percent of a city's income raises local income by 2.4 percent over the next two years. This multiplier effect is larger than estimates for military spending (1.5 on average). Multipliers are higher when grants are awarded to students at non-profit colleges, as for-profit colleges absorb most of the grant increases with raises in tuition. Multipliers are also higher during recessions than in expansions: Pell grants can be an effective tool for countercyclical policy that adds to already established benefits, such as, increasing the affordability of college and fostering long-run economic growth.
Jacques Ranciere’de Entelektüelin İşlevinin Sorgulanması: “Cahil Hoca” Okulda
Authors: EKİNCİ Özgül
Published: August 2020

Entelektüelin konumu sorunu, her zaman için ilgi çekici bir konu olmuştur. Çağdaş dönemde 1968 Olayları entelektüelin konumunun yeniden sorgulanmasına sebep olmuştur. İşçi ve öğrencilerin kendi içinde bulundukları durumu anlayıp hiçbir entelektüelin öncülüğüne ihtiyaç duymadan harekete geçmelerine sebep olan 1968 Olayları, entelektüelin konumu noktasında bazı soru işaretlerine neden olmuştur. Bu süreçte, Louis Althusser, Jean Paul Sartre, Michel Foucault, Edward Said ve Jacques Rancière entelektüelin konumu üzerine kafa yoran çağdaş düşünürler olmuşlardır. Althusser, Sartre ve Said entelektüelin hala ayrıcalıklı bir konum işgal ettiğine inanırken Foucault bu ayrıcalıklı konumu eleştiren bir okuma gerçekleştirir. Rancière ise bu düşünürlerden tamamen ayrılarak entelektüelin hiçbir ayrıcalıklı konuma sahip olmadığını iddia eder. Cahil Hoca, eserinde, Rancière bu iddiasını, bilgi ile otorite arasındaki bağları kopararak destekler. Hocanın bilgiye dayalı otoritesi yerine bunun aksi olarak cahil hoca figürüne yer veren Rancière, pedegojik süreç de bireylerin kendi zihinsel gücünü ön plana çıkarır. Zekaların eşitliği gibi oldukça radikal varsayımı ile oluşturduğu pedogoji de, öğrencinin öğrenmesinde hocanın etkin bir rolü yoktur. Filozof ve Yoksulları, Özgürleşen Seyirci gibi eserlerinde de birçok ayrımın varsayımsal olduğu üzerine odaklanır. Bu ayrımların beraberinde belirlediği üstün ve aşağı olma durumlarını reddeden Rancière, pedogojik, siyasi ve sanatsal otoritelerin yerine eşitliği bir ön varsayım olarak ortaya koyar. İleri sürülen bu eşitlik fikrinde, entelektüel için ayrıcalıklı hiçbir yer kalmamaktadır.
Kilis İlinin Lojistik Açıdan Değerlendirilmesi ve Kurulması Düşünülen Medine Pazarı’nın Şehre Etkileri
Authors: AKÇACI Taner , ÇINAROĞLU Mehmet Serkan , ÇAVUŞOĞLU Abdulvahid
Published: August 2020

Küreselleşme ve teknolojide büyük gelişmelerin yaşanması son yıllarda lojistik faaliyetlerinin ve lojistik sektörünün gelişiminde önemli rol oynamaktadır. Bu amaçla Amerika, Fransa ve İtalya gibi birçok ülkede etkin bir şekilde lojistik ile ilgili faaliyetlerin yürütüldüğü lojistik merkezler oluşturulmuştur. Sanayicilerin rekabet güçlerini artırmak ve Türkiye’yi bölgenin lojistik üssüne dönüştürmek üzere farklı yerlerde lojistik merkez yapımı planlanmıştır. Ülkemizde işletmeye açılan ilk lojistik merkezi Gelemen (Samsun)’dir. Bunu, Halkalı (İstanbul), Gökköy (Balıkesir), Kaklık (Denizli), Hasan Bey (Eskişehir), Köseköy (İzmit), Palandöken (Erzurum), Uşak ve Türkoğlu (Kahramanmaraş) lojistik merkezleri takip etmiştir. Bunun yanı sıra Kars ve Kemalpaşa/İzmir AYGM lojistik merkezlerinin yapımı devam etmektedir. Bu çalışmada genel olarak, Türkiye’de ve özellikle Kilis ilinde lojistik faaliyetleri tespit etmek ve Öncüpınarda kurulacak olan Medine Pazarı’nın ülkelerarası önemini ve il ekonomisine etkilerini ortaya koyulması amaçlanmıştır.
Effect of Wholewheat Flour Particle Shape Obtained by Different Milling Processes on Physicochemical Characteristics and Quality of Bread
Authors: Navarro José Luis , Moiraghi Malena , Quiroga Fernanda Micaela , León Alberto Edel , Steffolani María Eugenia
Published: August 2020

Research background. Wholewheat flour is a very good source of nutritional compounds and functional ingredients for human diet. Yet, its use causes negative effect on bread quality. Different milling techniques could be used to obtain wholewheat flour, minimizing the negative effect of both bran and germ on bread quality. The aim of this work was to study the effect of particle size and shape of wholegrain flour on the interaction between the different components, the water distribution, dough rheology and bread volume.
Experimental approach. Wholewheat flour of three varieties (Klein Rayo, Fuste, INTA815) were obtained by cyclonic, hammer and roller mills. The characteristics of wholewheat flour were explored, and the water distribution and rheological properties of dough were determined by thermogravimetric analysis and Mixolab test, respectively. Finally, microscale bread was prepared.
Results and conclusions. The amount of water-soluble pentosans, damaged starch and wet gluten was affected by the milling procedure. Regarding dough rheological properties, wholewheat flour by hammer mill had the lowest water absorption and the highest developing time. This result could be mainly attributed to particle shape in these samples with large amount of endosperm attached to the bran, hindering protein unfolding. Thermogravimetric analysis exhibit that both fine and large bran particle size seem to have the same effect on water properties in wholewheat dough during heating. Bread made with Klein Rayo had the highest specific volume, indicating that wheat with high protein content and breadmaking quality is needed to make wholewheat bread. The results of this work showed that particle shape, rather than particle size, affected the quality of wholewheat flour for breadmaking. Thus, the wholegrain milling process should be carefully selected taking into account the shape of particle produced.
Cytotoxicity assay using LLC-MK2 cell line v2
Authors: not provided luanaborba
Published: August 2020

Protocol that described the cytotoxicity assay using a mammalian epithelial cell line (LLC-MK2; ATCC CCL-7).
Conjugation of Keyhole limpet haemocynin to Peptide 254-274 of HIV gp-120 as immunogen. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Chemical synthesis facilitates the generation of peptides which are difficult to express in bacteria. The fragment 254-274 of the human immunodeficiency virus (HIV) is critical for infectivity of the virus and induction of antibody neutralization (Ho et al, 1988). Boudet et al, 1995 demonstrated that a synthetic peptide derived from the third variable domain of the HIV-1 gp-120 when used as immunogen was able to induce an antibody response to multiple (up to six) HIV strains. Reference Ho DD, Kaplan JC, Rackauskas IE, Gurney ME. Second conserved domain of gp120 is important for HIV infectivity and antibody neutralization.Science. 1988;239(4843):1021-1023. doi:10.1126/science.2830667. Boudet F, Keller H, Kieny MP, Thèze J. Single peptide and anti-idiotype based immunizations can broaden the antibody response against the variable V3 domain of HIV-1 in mice.Mol Immunol. 1995;32(7):449-457. doi:10.1016/0161-5890(95)00007-2
Tissue Cyclic Immunofluorescence (t-CyCIF) v2
Authors: Ren Lin Jia
Published: August 2020

The architecture of normal and diseased tissues strongly influences the development and progression of disease as well as responsiveness and resistance to therapy. We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immunofluorescence imaging of specimens mounted on glass slides. t-CyCIF generates up to 60-plex images using an iterative process (a cycle) in which conventional low-plex fluorescence images are repeatedly collected from the same sample and then assembled into a high dimensional representation. t-CyCIF requires no specialized instruments or reagents and is compatible with super-resolution imaging; we demonstrate its application to quantifying signal transduction cascades, tumor antigens and immune markers in diverse tissues and tumors. The simplicity and adaptability of t-CyCIF makes it an effective method for pre-clinical and clinical research and a natural complement to single-cell genomics. Key resources, reagents, and software are listed at the HMS LINCS Center Publication Page http://lincs.hms.harvard.edu/lin-elife-2018/ (RRID:SCR_016370).This page provides links to an OMERO image database from which individual images can be obtained; stitched and registered image panels can be obtained at www.cycif.org (RRID:SCR_016267) and a video illustrating the t-CyCIF method can be found at https://vimeo.com/269885646. This protocol is used in the following manuscripts: Lin J-R, Izar B, Wang S, Yapp C, Mei S, Shah P, Santagata S, Sorger PK. (2018). Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. eLife. PMID: 29993362 Du Z, Lin JR, Rashid R, Maliga Z, Wang S, Aster J, Izar B, Sorger PK, Santagata S. (2019). Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging. Nature Protocols. PMID: 31534232 The original t-CyCIF protocol can be found at dx.doi.org/10.17504/protocols.io.rpxd5pn.
Polson method for IgY isolation with modification. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Immunoglobulin Y (IgY) is the mayormolecule present in the avian egg yolk. This protein functions as antibody and fulfils important functions in the protection of the embryo against challenging stimuli [1]. IgY has been isolated from the egg yolk of many birds [1]. These antibodies are important therapeutically, but also they have been used as immunological tool in many immunodiagnostic assays. Much time IgY concentration is calculated from the water soluble fraction (WSF), which means that there is not need of treating the preparation with alcohol to separate the protein pellet. Reference 1. Justiz Vaillant AA, Ramirez N, Cadiz A, Ferrer B, Akpaka P, et al. (2013) Separation and Reactivity of Avian Immunoglobulin Y. J Chromat Separation Techniq 4: 173. doi:10.4172/2157-7064.1000173
Using Concept Cartoons to Identify the Epistemological Beliefs of Middle School Students
Authors: Atasoy Şengül
Published: August 2020

Epistemological beliefs, including the source of knowledge, the certainty of knowledge, the organization of knowledge, the control of learning, and the speed of learning, are important to identify since these beliefs impact students' learning processes and how they attribute meaning to life. Concept cartoons, that students find genuine and credible, are found to be effective assessment tools in revealing these beliefs. The present study aims to identify and compare 5th and 8th grade middle school students' epistemological beliefs utilizing concept cartoons. The study participants were 5th (N=38) and 8th grade (N=47) students enrolled in three different middle schools in the Çamlıhemşin district of Rize. In order to reveal the students' opinions, one concept cartoon for each dimension of epistemological belief was utilized. A scoring rubric was employed to analyze the student responses to the concept cartoons. According to the results of the t-tests on students' scores, the 5th and 8th grade students' epistemological beliefs in the dimensions of organization of knowledge, source of knowledge, and certainty of knowledge are similar. In contrast, 8th grade students' epistemological beliefs related to the speed of learning and the control of learning were found to be significantly higher than those of 5th-grade students. Among the mean values of the students' epistemological beliefs, source of knowledge beliefs were found to be the lowest.Keywords Concept Cartoons, Epistemological Beliefs, Middle School Students
Un análisis de las formas de trato desde un enfoque sociolingüístico
Authors: Bestard Revilla Alina
Published: August 2020

El estudio reflexionó acerca del movimiento experimentado en el eje poder/solidaridad, que afecta el uso de las formas de tratamiento en una comunidad urbana de Santiago de Cuba, relacionado con las transformaciones socioeconómicas de las últimas décadas en Cuba. Luego de la recolección de datos realizada y de su confrontarse por triangulación de datos tomados de otras zonas santiagueras, fue posible la conformación de un corpus lingüístico. Los resultados indican que el movimiento está vinculado con los conceptos emergentes de poder, relacionados con el estatus socio-económico de los hablantes. Se expondrán los orígenes, usos y significados de tales conceptos.
Las ideas de Gabriel García Márquez sobre el diccionario y el léxico del español: un análisis del «prólogo» al Clave: Diccionario de uso del español actual
Authors: Cabello Pino Manuel
Published: August 2020

Gabriel García Márquez mantuvo a lo largo de su vida una intensa relación con el diccionario, que le llevó a desarrollar sus propias ideas respecto al mismo, las cuales no dudó en plasmar de forma bastante dispersa en distintos textos. En este trabajo se llevó a cabo un análisis pormenorizado de uno de esos textos fundamentales: el «Prólogo» al Clave. Diccionario de uso del español actual, para comprobar si sus ideas sobre el diccionario evolucionaron de alguna manera con respecto a lo que ya había expresado quince años antes en algunas de sus famosas notas de prensa de los ochenta.
Relaciones entre aversión al riesgo y sobrecarga en la elección
Authors: Villa Bedoya David Felipe
Published: August 2020

Este artículo presenta un análisis de correlaciones entre los indicadores observables de la aversión al riesgo y la sobrecarga en la elección, en una muestra conformada por 120 estudiantes universitarios. Los resultados indican diversas correlaciones en el indicador tiempo, y diferencias signiﬁcativas por género. El tiempo, al ser interpretado de manera aislada en investigaciones antecedentes, sustentaba explicaciones unidireccionales que tomaban al decisor como causa de las decisiones, en algunos casos, y al entorno, en otros. Se presentan dos niveles de análisis: (1) análisis de lo visible, que incluye consideraciones relacionadas directamente con los indicadores observables medidos y sus resultados, y (2) análisis de lo no visible, donde se establecen preguntas por el nivel de trascendencia del dato visible, se esboza el entendimiento de las situaciones decisorias como sistemas complejos de información sujetos al cambio y se discute la tendencia a explicar unidireccionalmente las causas del comportamiento en determinadas situaciones decisorias o contextos.
El “común” y su “vana filosofía”. Formación de sistemas de conocimiento alternos en el virreinato de la Nueva Granada
Authors: Peralta Agudelo Jaime Andrés
Published: August 2020

El devenir científico en la América colonial se ha estudiado generalmente a partir de los contenidos de la ciencia moderna que fluyeron desde Europa hacia las posesiones ultramarinas, por lo que se ha omitido que entre los pueblos amerindios, africanos, mestizos y todas sus “castas” intermedias también hubo espacio para la creación intelectual. Este artículo allana el camino para encontrar algunas pistas sobre la existencia de aquellas matrices de pensamiento diferenciales, así como de los protocolos, conceptos, productos y sus artífices que permitieron desarrollar alternativas de bienestar propias y puntos divergentes al orden de dominación instaurado por el régimen colonial durante el siglo XVIII.
Wilhelm Vallentin en Argentina (1904-1905): proyectos de colonización entre actores locales y una trayectoria global
Authors: Olivera Gastón
Published: August 2020

Se presentan nuevos materiales sobre la vida del explorador alemán Wilhelm Vallentin con el objeto de conocer su itinerario, encuentros y proyectos en Argentina. Por medio de sus propios relatos de viaje, de la prensa periódica de habla alemana editada en Buenos Aires y de diverso material de archivo se ilustra la densa red social que la colectividad germanoparlante desplegó en ese país desde fines del siglo XIX. A través de esta trayectoria singular se espera mostrar el potencial de la escritura biográfica como medio para comprender una coyuntura específica de la historia global en sus articulaciones regionales y locales.
New Public Management: US Experience for Ukraine
Authors: Khomytskyi Vitalii
Published: August 2020

Purpose. The purpose of the article is to analyse the effectiveness of reforms based on new public management concept in the United States and identify key elements for implementation in Ukraine.
Methodology. The results of the study were obtained by the following methods: systematic and comparative methods - to determine the nature and features of the historical formation of new public management reforms in the United States, based on understanding administrative activities through the prism of private economy, public service orientation on efficiency and effectiveness; methods of analysis and synthesis - to identify complex historical factors and interests that led to the formation and functioning of the new public management system.
Findings. In accordance with the study objectives, the author:
1) established and analysed the theoretical sources and methodological principles of the study of the concept of new public administration by T. Goebler and D. Osborne;
2) clarified the significance of the concept of the new public administration of T. Goebler and D. Osborne for the system of modern scientific research of the public sector;
3) revealed the meaning of the concept of new public administration as an administrative process;
4) analysed the problems of public administration efficiency in the context of the concept of new public management during the reforms in the United States;
5) identified the possibilities of applying the concept of new public management in carrying out reforms of modern administration processes in Ukraine.
Originality. Author has proposed vision and interpretation of historical factors that led to the formation of a new public management. The article contains an analysis of the historical events and the practical consequences of the reforms in the United States.
Practical value. Regarding the government change in Ukraine and the volatile economic situation due to the coronavirus pandemic, the implementation of elements of new public management concept might help increase the effectiveness of civil service reform in Ukraine. The materials of the article can be used in the practice of public administration; in the development of courses in public administration; to improve training programs and plans for government officials; in preparation of textbooks, educational and methodical manuals.
The Role of Theory in the Study of Legal Regulation of Public Procurement
Authors: Shmeleva M. V.
Published: August 2020

Public procurement is a mainstream of today. We can observe as the scientific field of public procurement continues to expand, since academicians in law, public administration, finance, management and information technology tend to apply their knowledge to study public procurement. Progress in public procurement is equally evident in the political and economic fields, where public procurement is closely linked to problems of social integration, economic growth and environmental sustainability. This raises important questions as to how and by which methods we should research this area. Based on the results of the study, we conclude that applying existing theories to practical challenges has great potential to generate new possible ways of solving problems in the field of public procurement. At the same time the scholars’ will help generate data for the development of new ideas, concepts and even certain theories in the field of public procurement.
Cryptocurrency as a Digital Representation of Value: The Singapore Experience
Authors: Alekseenko A. P. , Belykh V. S.
Published: August 2020

In 2019, in order to create attractive conditions for the development of the Fintech sector in Singapore, the Payment Services Law was adopted, which consolidated the definition of cryptocurrency (digital payment tokens) and the procedure for its circulation. In addition, this Law established the criteria for distinguishing payment tokens from other virtual objects — securities tokens, utility tokens, game currencies. In the paper, the authors analyze the concept of digital payment tokens as a digital representation of value, consider the rules for transactions with them. It is concluded that the use of this approach makes it possible to minimize difficulties in regulating the turnover of, for example, bitcoins. Based on the Singapore experience, recommendations for the modernization of domestic legislation are proposed. The main difficulty seems to be that the use of the digital value representation mode in relation to cryptocurrency is associated with the need to either establish an extraterritorial regime of regulation in relation to cryptocurrency exchanges, or adopt an international agreement in this area.