bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Omega-3 Polyunsaturated Fatty Acids Treatment on Lipid Pattern of HIV Patients: A Meta-Analysis of Randomized Clinical Trials
Authors: Fogacci Federica , Strocchi Enrico , Veronesi Maddalena , Borghi Claudio , Cicero Arrigo F. G.
Published: June 2020

Even though omega-3 polyunsaturated fatty acids (PUFAs) seem to be effective in the treatment of human immunodeficiency virus (HIV)-associated dyslipidemia, their impact is still debated. For this reason, our aim was to perform a meta-analysis of the clinical evidence available to date. A systematic literature search was conducted in order to identify published clinical trials assessing the effect of PUFAs treatment on serum lipoproteins, and its safety profile. The effect sizes for lipid changes were expressed as mean difference (MD) and 95% confidence interval (CI). For safety analysis, odd ratios and the 95% CI were calculated with the Mantel–Haenszel method. Data were pooled from nine clinical studies comprising overall 578 HIV-affected subjects. Meta-analysis of the data suggested that omega-3 PUFAs significantly reduced triglycerides (TG) (MD = −1.04, 95% CI: −1.5, −0.58 mmol/L, p < 0.001), while increasing high-density lipoprotein cholesterol (MD = 0.36, 95% CI: 0.12, 0.61 mmol/L, p = 0.004), without affecting serum levels of total cholesterol, very-low- and low-density lipoprotein cholesterol, and apolipoprotein B and A1. Change in TG was significantly associated with eicosapentaenoic acid administered via daily dose. PUFA treatment did not lead to an increased risk of adverse events. In conclusion, PUFAs are safe and exert a significant plasma lipid improving effect in HIV-positive patients.
Non-Steroidal Anti-Inflammatory Drug Etoricoxib Facilitates the Application of Individualized Exercise Programs in Patients with Ankylosing Spondylitis
Authors: Marcu Iulia Rahela , Dop Dalia , Padureanu Vlad , Niculescu Stefan Adrian , Padureanu Rodica , Niculescu Carmen Elena , Rogoveanu Otilia Constantina
Published: June 2020

Background and objectives: The main objective of this study is to highlight the efficiency of different therapeutic means in patients with ankylosing spondylitis, resulting in the improvement of their quality of life. Materials and Methods: We conducted a randomized, longitudinal, controlled trial on 92 patients with ankylosing spondylitis over a period of 6 years. Disease activity was assessed using the BASDAI (Bath Ankylosing Spondylitis Disease Activity Index) score. The assessment of functional disabilities was performed using BASFI (Bath Ankylosing Spondylitis Functional Index). We assessed the quality of life using the HAQ questionnaire (Health Assessment Questionnaire). Based on the HAQ, we calculated the minimum number of patients to be treated for 52 weeks to prevent a decrease in the quality of life for at least one of them (the number needed to treat (NNT)). Results: For the combination therapy group, the result we obtained was 2, lower than the other therapies compared (the medication group and the group with physical exercise). We point out a correlation between the improvement of the functional status (BASFI) and the increase of the quality of life (HAQ), estimated as moderately high (0.8). The superiority of the effects of the combined treatment, in which we combined a nonsteroidal anti-inflammatory drug (etoricoxib) to the exercise program, is reflected by the model of the significant improvements (p < 0.05) obtained for the functional status and quality of life scores (BASFI and HAQ). Conclusions: The nonsteroidal anti-inflammatory drugs, in our case, etoricoxib, facilitate the application of individualized exercise programs in patients with ankylosing spondylitis.
Effectiveness of Integrated Health Systems in Africa: A Systematic Review
Authors: Ampomah Irene G. , Malau-Aduli Bunmi S. , Malau-Aduli Aduli E.O. , Emeto Theophilus I.
Published: June 2020

Background and objective: Traditional medicine (TM) was integrated into health systems in Africa due to its importance within the health delivery setup in fostering increased health care accessibility through safe practices. However, the quality of integrated health systems in Africa has not been assessed since its implementation. The objective of this paper was to extensively and systematically review the effectiveness of integrated health systems in Africa. Materials and Methods: A systematic literature search was conducted from October, 2019 to March, 2020 using Ovid Medline, Scopus, Emcare, Web of Science, Cumulative Index to Nursing and Allied Health (CINAHL), and Google Scholar, in order to retrieve original articles evaluating the integration of TM into health systems in Africa. A quality assessment of relevant articles was also carried out using the Quality Assessment Tool for Studies with Diverse Designs (QATDSS) critical appraisal tool. Results: The results indicated that the formulation and execution of health policies were the main measures taken to integrate TM into health systems in Africa. The review also highlighted relatively low levels of awareness, usage, satisfaction, and acceptance of integrated health systems among the populace. Knowledge about the existence of an integrated system varied among study participants, while satisfaction and acceptance were low among orthodox medicine practitioners. Health service users’ satisfaction and acceptance of the practice of an integrated health system were high in the countries assessed. Conclusion: The review concluded that existing health policies in Africa are not working, so the integration of TM has not been successful. It is critical to uncover the barriers in the health system by exploring the perceptions and experiences of stakeholders, in order to develop solutions for better integration of the two health systems.
Periodontitis, Low-Grade Inflammation and Systemic Health: A Scoping Review
Authors: Cecoro Gennaro , Annunziata Marco , Iuorio Morena Tina , Nastri Livia , Guida Luigi
Published: June 2020

Background and objectives: Periodontitis is a multifactorial chronic inflammatory infectious disease in which an infection is necessary, but not sufficient, for development of the condition. Individual susceptibility strictly linked to the immune and inflammatory response of the organism must also be present. Low-grade inflammation (LGI) is a systemic status of chronic sub-clinical production of inflammatory factors. This condition represents a risk factor for many chronic diseases including diabetes, cardiovascular disease, cerebrovascular disease, neurodegenerative disease and cancer. This scoping review aims to clarify, summarize and disseminate current knowledge on the possible link between periodontitis, LGI and systemic health. Materials and Methods: PRISMA Extension for Scoping Reviews guidelines were followed. An ad-hoc created keyword string was used to search the electronic databases of PubMed/Medline, Embase, The Cochrane Library and ClinicalTrials.gov. A hand search of specialized journals and their reference lists was also performed. Results: 14 studies that respected eligibility criteria were selected and analyzed. There is emerging evidence of strong links between periodontitis, LGI and systemic health. On the one hand, periodontitis influences the systemic status of LGI and on the other hand, the systemic production of inflammatory factors affects periodontitis with a bidirectional connection. Conclusions: LGI and the subsequent onset of a systemic inflammatory phenotype can be considered the common substrate of many chronic inflammatory diseases including periodontitis, with multiple mutual connections between them. Understanding of the biological principles and mechanisms underlying such a complex interrelationship could lead to significant improvements in the field of personalized diagnostics and therapeutic protocols.
Genista tridentata L.: A Rich Source of Flavonoids with Anti-Inflammatory Activity
Authors: Pinto Diana C. G. A. , Simões Mark A. M. , Silva Artur M. S.
Published: June 2020

Background: Genista tridentata L. is an endemic species from the Iberian Peninsula used in Portuguese traditional medicine to treat inflammation-related diseases; this and other health-promoting effects are usually associated with the flavonoids produced by this species. In fact, anti-inflammatory properties were established for several of these flavonoid derivatives. Methods: A careful survey of the reported data, using mainly the Scopus database and Genista tridentata and Pterospartum tridentatum as keywords, was done. We have examined the papers involving the plant and those about the most relevant flavonoids anti-inflammatory activity. Results: The literature survey demonstrates that species are used to treat several health problems such as antihyperglycemia, hypertension, and inflammatory episodes. It was also possible to establish its richness in flavonoid derivatives, from which several are potential anti-inflammatory agents. Conclusions: From our described and discussed analysis, it can be concluded that Genista tridentata is an excellent source of bioactive flavonoids. Moreover, its traditional use to treat inflammation episodes may be due to its flavonoid content, from which genistein, biochanin A, rutin, and daidzein can be emphasized.
Mössbauer Analysis of Deformation–Induced Acceleration of Short-Range Concentration Separation in Fe-Cr Alloys—Effect of the Substitution Impurity: Sb and Au
Authors: Shabashov Valery , Kozlov Kirill , Ustyugov Yurii , Zamatovskii Andrey , Tolmachev Timofey , Novikov Evgenii
Published: June 2020

The effect of doping the ferrite alloy Fe-16Cr by the oversized impurities Sb and Au on the mechanism of the short-range ordering induced by “warm” severe plastic deformation was studied using the method of Mössbauer spectroscopy. A comparison between the results obtained and the positron annihilation data on the evolution of the defects of vacancy type stabilized by the impurities Sb and Au was performed. It has been established that the impurities Sb and Au entail a shift of the temperature region of short-range ordering realization in conditions of applying pressure torsion towards greater temperatures by 250 and 100 K, respectively.
Brief Review on Experimental and Computational Techniques for Characterization of Cellular Metals
Authors: Duarte Isabel , Fiedler Thomas , Krstulović-Opara Lovre , Vesenjak Matej
Published: June 2020

The paper presents a brief review of the main experimental and numerical techniques and standards to investigate and quantify the structural, mechanical, thermal, and acoustic properties of cellular metals. The potential of non-destructive techniques, such as X-ray computed tomography and infrared thermography are also presented.
Implementation of a Single Emulsion Mask for Three-Dimensional (3D) Microstructure Fabrication of Micromixers Using the Grayscale Photolithography Technique
Authors: Abdul Hamid Intan Sue Liana , Khi Khim Beh , Sal Hamid Sofiyah , Abd Rahman Mohamad Faizal , Abd Manaf Asrulnizam
Published: June 2020

Three-dimensional (3D) microstructures have been exploited in various applications of microfluidic devices. Multilevel structures in micromixers are among the essential structures in microfluidic devices that exploit 3D microstructures for different tasks. The efficiency of the micromixing process is thus crucial, as it affects the overall performance of a microfluidic device. Microstructures are currently fabricated by less effective techniques due to a slow point-to-point and layer-by-layer pattern exposure by using sophisticated and expensive equipment. In this work, a grayscale photolithography technique is proposed with the capability of simultaneous control on lateral and vertical dimensions of microstructures in a single mask implementation. Negative photoresist SU8 is used for mould realisation with structural height ranging from 163.8 to 1108.7 µm at grayscale concentration between 60% to 98%, depending on the UV exposure time. This technique is exploited in passive micromixers fabrication with multilevel structures to study the mixing performance. Based on optical absorbance analysis, it is observed that 3D serpentine structure gives the best mixing performance among other types of micromixers.
3D Printed Biomodels for Flow Visualization in Stenotic Vessels: An Experimental and Numerical Study
Authors: Carvalho Violeta , Rodrigues Nelson , Ribeiro Ricardo , Costa Pedro F. , Lima Rui A. , F.C.F. Teixeira Senhorinha
Published: June 2020

Atherosclerosis is one of the most serious and common forms of cardiovascular disease and a major cause of death and disability worldwide. It is a multifactorial and complex disease that promoted several hemodynamic studies. Although in vivo studies more accurately represent the physiological conditions, in vitro experiments more reliably control several physiological variables and most adequately validate numerical flow studies. Here, a hemodynamic study in idealized stenotic and healthy coronary arteries is presented by applying both numerical and in vitro approaches through computational fluid dynamics simulations and a high-speed video microscopy technique, respectively. By means of stereolithography 3D printing technology, biomodels with three different resolutions were used to perform experimental flow studies. The results showed that the biomodel printed with a resolution of 50 μm was able to most accurately visualize flow due to its lowest roughness values (Ra = 1.8 μm). The flow experimental results showed a qualitatively good agreement with the blood flow numerical data, providing a clear observation of recirculation regions when the diameter reduction reached 60%.
Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
Authors: Driessen Rob , Zhao Feihu , Hofmann Sandra , Bouten Carlijn , Sahlgren Cecilia , Stassen Oscar
Published: June 2020

Endothelial cells sense and respond to shear stress. Different in vitro model systems have been used to study the cellular responses to shear stress, but these platforms do not allow studies on high numbers of cells under uniform and controllable shear stress. The annular dish, or dish-in-a-dish (DiaD), on the orbital shaker has been proposed as an accessible system to overcome these challenges. However, the influence of the DiaD design and the experimental parameters on the shear stress patterns is not known. In this study, we characterize different designs and experimental parameters (orbit size, speed and fluid height) using computational fluid dynamics. We optimize the DiaD for an atheroprotective flow, combining high shear stress levels with a low oscillatory shear index (OSI). We find that orbit size determines the DiaD design and parameters. The shear stress levels increase with increasing rotational speed and fluid height. Based on our optimization, we experimentally compare the 134/56 DiaD with regular dishes for cellular alignment and KLF2, eNOS, CDH2 and MCP1 expression. The calculated OSI has a strong impact on alignment and gene expression, emphasizing the importance of characterizing shear profiles in orbital setups.
An Immune–Magnetophoretic Device for the Selective and Precise Enrichment of Circulating Tumor Cells from Whole Blood
Authors: Chelakkot Chaithanya , Ryu Jiyeon , Kim Mi Young , Kim Jin-Soo , Kim Dohyeong , Hwang Juhyun , Park Sung Hoon , Ko Seok Bum , Park Jeong Won , Jung Moon Youn , Kim Ryong Nam , Song Kyoung , Kim Yu Jin , Choi Yoon-La , Lee Hun Seok , Shin Young Kee
Published: June 2020

Here, we validated the clinical utility of our previously developed microfluidic device, GenoCTC, which is based on bottom magnetophoresis, for the isolation of circulating tumor cells (CTCs) from patient whole blood. GenoCTC allowed 90% purity, 77% separation rate, and 80% recovery of circulating tumor cells at a 90 μL/min flow rate when tested on blood spiked with epithelial cell adhesion molecule (EpCAM)-positive Michigan Cancer Foundation-7 (MCF7) cells. Clinical studies were performed using blood samples from non-small cell lung cancer (NSCLC) patients. Varying numbers (2 to 114) of CTCs were found in each NSCLC patient, and serial assessment of CTCs showed that the CTC count correlated with the clinical progression of the disease. The applicability of GenoCTC to different cell surface biomarkers was also validated in a cholangiocarcinoma patient using anti-EPCAM, anti-vimentin, or anti-tyrosine protein kinase MET (c-MET) antibodies. After EPCAM-, vimentin-, or c-MET-positive cells were isolated, CTCs were identified and enumerated by immunocytochemistry using anti-cytokeratin 18 (CK18) and anti-CD45 antibodies. Furthermore, we checked the protein expression of PDL1 and c-MET in CTCs. A study in a cholangiocarcinoma patient showed that the number of CTCs varied depending on the biomarker used, indicating the importance of using multiple biomarkers for CTC isolation and enumeration.
Modeling a Dielectrophoretic Microfluidic Device with Vertical Interdigitated Transducer Electrodes for Separation of Microparticles Based on Size
Authors: Alnaimat Fadi , Mathew Bobby , Hilal-Alnaqbi Ali
Published: June 2020

This article conceptualizes and mathematically models a dielectrophoretic microfluidic device with two sets of interdigitated transducer vertical electrodes for separation of a binary heterogeneous mixture of particles based on size; each set of electrodes is located on the sidewalls and independently controllable. To achieve separation in the proposed microfluidic device, the small microparticles are subjected to positive dielectrophoresis and the big microparticles do not experience dielectrophoresis. The mathematical model consists of equations describing the motion of each microparticle, fluid flow profile, and electric voltage and field profiles, and they are solved numerically. The equations of motion take into account the influence of phenomena, such as inertia, drag, dielectrophoresis, gravity, and buoyancy. The model is used for a parametric study to understand the influence of parameters on the performance of the microfluidic device. The parameters studied include applied electric voltages, electrode dimensions, volumetric flow rate, and number of electrodes. The separation efficiency of the big and small microparticles is found to be independent of and dependent on all parameters, respectively. On the other hand, the separation purity of the big and small microparticles is found to be dependent on and independent of all parameters, respectively. The mathematical model is useful in designing the proposed microfluidic device with the desired level of separation efficiency and separation purity.
Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
Authors: Sun Wei-Sheng , Lee Yuarn-Jang , Tsai Kun-Nan , Ho Yu-Hsuan , Fang Shiuh-Bin
Published: June 2020

The prevalence of vancomycin resistant enterococcus (VRE) carrier-state has been increasing in patients of intensive care unit and it would be a public health threat. Different research groups conducted decolonizing VRE with probiotic and the results were controversial. Therefore, a systemic approach to search for the probiotic species capable of decolonizing VRE is necessary. Thus, VRE was co-cultured with ten probiotic species. The fluctuations of each bacterial population were analyzed by 16S rRNA sequencing. Microbial network analysis (MNA) was exploited to identify the most critical species in inhibiting the VRE population. The MNA-selected probiotic cocktail was then validated for its efficacy in inhibiting VRE, decolonizing VRE from Caco-2 cells via three approaches: exclusion, competition, and displacement. Finally, the expression of VRE virulence genes after co-incubation with the probiotic cocktail were analyzed with quantitative real-time PCR (qRT-PCR). The MNA-selected probiotic cocktail includes Bacillus coagulans, Lactobacillus rhamnosus GG, Lactobacillus reuteri, and Lactobacillus acidophilus. This probiotic combination significantly reduces the population of co-cultured VRE and prevents VRE from binding to Caco-2 cells by down-regulating several host-adhesion genes of VRE. Our results suggested the potential of this four-strain probiotic cocktail in clinical application for the decolonization of VRE in human gut.
Pathogenesis-Targeted Preventive Strategies for Multidrug Resistant Ventilator-Associated Pneumonia: A Narrative Review
Authors: Cotoia Antonella , Spadaro Savino , Gambetti Guido , Koulenti Despoina , Cinnella Gilda
Published: June 2020

Ventilator-associated pneumonia (VAP) is the most common hospital-acquired infection in the intensive care unit (ICU), accounting for relevant morbidity and mortality among critically ill patients, especially when caused by multidrug resistant (MDR) organisms. The rising problem of MDR etiologies, which has led to a reduction in treatment options, have increased clinician’s attention to the employment of effective prevention strategies. In this narrative review we summarized the evidence resulting from 27 original articles that were identified through a systematic database search of the last 15 years, focusing on several pathogenesis-targeted strategies which could help preventing MDR-VAP. Oral hygiene with Chlorhexidine (CHX), CHX body washing, selective oral decontamination (SOD) and/or digestive decontamination (SDD), multiple decontamination regimens, probiotics, subglottic secretions drainage (SSD), special cuff material and shape, silver-coated endotracheal tubes (ETTs), universal use of gloves and contact isolation, alcohol-based hand gel, vaporized hydrogen peroxide, and bundles of care have been addressed. The most convincing evidence came from interventions directly addressed against the key factors of MDR-VAP pathogenesis, especially when they are jointly implemented into bundles. Further research, however, is warranted to identify the most effective combination.
Dry-Caribbean Bacillus spp. Strains Ameliorate Drought Stress in Maize by a Strain-Specific Antioxidant Response Modulation
Authors: Moreno-Galván Andres , Romero-Perdomo Felipe A. , Estrada-Bonilla German , Meneses Carlos Henrique Salvino Gadelha , Bonilla Ruth R.
Published: June 2020

Drought is a global problem for crop productivity. Therefore, the objective of this research was to evaluate five dry-Caribbean Bacillus spp. strains in drought stress amelioration in maize plants. Maize seeds were single-strain inoculated and sown in pots under greenhouse conditions. After 12 days, plants were subjected to 33 days of drought conditions, i.e., 30% of soil field capacity, and then collected to measure leaf and root dry biomass, plant height, antioxidant enzymes, proline accumulation, and P+, Ca2+, and K+ uptake. Results correlated drought stress amelioration with the inoculation of Bacillus spp. strains XT13, XT38 and XT110. Inoculated plants showed increases in dry biomass, plant height, and K+ and P+ uptake. The overall maize antioxidant response to bacterial inoculation under drought stress showed dependence on proline accumulation and decreases in ascorbate peroxidase and glutathione reductase activities. Moreover, results suggest that this stress amelioration is driven by a specific plant-strain correlation observed in antioxidant response changes in inoculated plants under stress. Also, there is a complex integration of several mechanisms, including plant growth-promotion traits and nutrient uptake. Hence, the use of dry-Caribbean plant growth-promoting Bacillus strains represents an important biotechnological approach to enhance crop productivity in arid and semi-arid environments.
Part–Whole Relations: New Insights about the Dynamics of Complex Geochemical Riverine Systems
Authors: Gozzi Caterina , Sauro Graziano Roberta , Buccianti Antonella
Published: June 2020

Nature is often characterized by systems that are far from thermodynamic equilibrium, and rivers are not an exception for the Earth’s critical zone. When the chemical composition of stream waters is investigated, it emerges that riverine systems behave as complex systems. This means that the compositions have properties that depend on the integrity of the whole (i.e., the composition with all the chemical constituents), properties that arise thanks to the innumerable nonlinear interactions between the elements of the composition. The presence of interconnections indicates that the properties of the whole cannot be fully understood by examining the parts of the system in isolation. In this work, we propose investigating the complexity of riverine chemistry by using the CoDA (Compositional Data Analysis) methodology and the performance of the perturbation operator in the simplex geometry. With riverine bicarbonate considered as a key component of regional and global biogeochemical cycles and Ca2+ considered as mostly related to the weathering of carbonatic rocks, perturbations were calculated for subsequent couples of compositions after ranking the data for increasing values of the log-ratio ln(Ca2+/HCO3−). Numerical values were analyzed by using robust principal component analysis and non-parametric correlations between compositional parts (heat map) associated with distributional and multifractal methods. The results indicate that HCO3−, Ca2+, Mg2+ and Sr2+ are more resilient, thus contributing to compositional changes for all the values of ln(Ca2+/HCO3−) to a lesser degree with respect to the other chemical elements/components. Moreover, the complementary cumulative distribution function of all the sequences tracing the compositional change and the nonlinear relationship between the Q-th moment versus the scaling exponents for each of them indicate the presence of multifractal variability, thus revealing scaling properties of the fluctuations.
The Impact of Microstructure of Filtration Materials on Its Auto-Activation for Manganese Removal from Groundwater
Authors: Dymaczewski Zbysław , Falkowska Joanna , Frąckowiak Angelika , Jeż-Walkowiak Joanna , Nawrot Justyna , Dudek Lidia , Topór Tomasz
Published: June 2020

The study investigates the impact of pore structure parameters of three chemically non-active filtration materials on the auto-activation time and the height of the filtration zone for manganese removal from groundwater. At a technical scale, the activation is a long process which may require a period of up to three months. The process can be shortened by the use of porous filtration materials. In this study, three filtration materials (silica sand, chalcedonite sand, GAC—granulated activated carbon) were investigated using subcritical nitrogen adsorption and mercury injection capillary pressure measurements. These methods provide a comprehensive evaluation of pore structure parameters, including specific surface area (SSA), micro- and mesopore volumes, and an extended range of pore size distribution (PSD). The studied materials provided a wide range of micro- and mesopore volumes as well as SSA. In order to investigate the auto-activation time of filtration material—the time required to achieve the requested quality of the filtrate—and the height of the filtration zone, a pilot experiment was conducted for 1950 h. The pilot installation consisted of three-meters-high (10 cm diameter) filtration columns filled with the tested filtration materials. The results indicate that the internal pore structure and the investigated auto-activation parameters are correlated. Both auto-activation time and the size of the filtration zone were influenced by the mesopore volume and the associated SSA of the tested materials. The micropore volume was less effective in improving the autoactivation parameters. The research results indicated the importance of pore structure characteristics which should be considered prior to time-consuming pilot and technological tests.
Petrological and Geochemical Properties of Greek Carbonate Stones, Associated with Their Physico-Mechanical and Aesthetic Characteristics
Authors: Badouna Ioanna , Koutsovitis Petros , Karkalis Christos , Laskaridis Konstantinos , Koukouzas Nikolaos , Tyrologou Pavlos , Patronis Michalis , Papatrechas Christos , Petrounias Petros
Published: June 2020

Greece is considered amongst the world’s top marble producers in the global carbonate ornamental stone market. Selected Greek carbonate ornamental stones considered in our study suite are characterized by their distinctive and in some cases unique appearance, having a significant impact on their commercial value. Their wide range of colour varieties and their physico-mechanical properties are closely related to their mineral assemblage, chemical constitution, petrographic properties, structural defects, which in turn depend highly upon their metamorphic/diagenetic grade and in some cases hydrothermal processes that affected them. This study endeavors to feature the petrographic, mineralogical and geochemical properties of the main Greek carbonate ornamental stones from selected localities and their by-product waste material used as aggregates. The documented data aims to serve a better understanding of the dynamic Greek marble industry by relating their mineral and chemical properties with their physico-mechanical and aesthetic characteristics.
Neoproterozoic Nafun Group Sediments from Oman Affected by an Active Continental Margin
Authors: Yue Liang , Vandeginste Veerle
Published: June 2020

The Neoproterozoic era is a time of major environmental change in Earth history. The Ediacaran period (635–541 Ma), the uppermost division of Precambrian time, is characterized by the remarkable Shuram excursion (largest C isotope negative excursion), a deep ocean water oxidation event, and Ediacaran biota. The Nafun Group of Oman provides a well-preserved and mostly continuous section of an Ediacaran succession. Based on geochemical data from the Nafun Group, the Shuram excursion (SE) and deep ocean oxidation hypotheses were proposed. Now, we sampled this section at high stratigraphic resolution, and present here the petrographical and geochemical analysis of the Khufai, Shuram and Buah Formations. The major and trace element analysis of shales from the Shuram Formation indicates that northern Oman was an active continental margin environment in Neoproterozoic times. The provenance of the Shuram Formation was primarily mafic and intermediate igneous rocks. With the unsteady tectonic setting, the development of the Nafun Group was influenced by hydrothermal supply and volcaniclastic input. Based on the V/Cr and U/Th ratio of the samples from the Nafun Group, our study reveals the transition of the ocean water redox environment, which is connected to the rise and fall of the Ediacaran biota. Our study constrains the tectonic setting of northern Oman and the petrography and geochemical data from the Nafun Group for the hydrothermal and volcaniclastic supply. Thus, our study acknowledges more factors for the explanation of the Ediacaran conundrums.
Comparison of Usability between Gear Shifters with Varied Visual and Haptic Patterns and Complexities
Authors: Lohilahti Bladfält Sanna , Grane Camilla , Bengtsson Peter
Published: June 2020

Shift-by-wire technology enables more options concerning the design, placement and functions of gear shifters compared to traditional gear shifters with manual transmission. These variations can impact usability and driver performance. There is a lack of research regarding the potential advantages and disadvantages of different types of gear shifters. The purpose of this study was to investigate the efficiency and subjective ease-of-use of mono- and polystable joystick gear shifter types at different complexity levels and with full or limited visibility. An experimental study with 36 participants was conducted. The results showed that monostable joysticks, especially those with an I/J-shape, were overall less efficient and easy to use than polystable joysticks. The highest complexity level clearly affected the efficiency for the monostable joystick with an I/J-shape (mono I/J) compared with the other gear shifter types. The monostable joystick with an I/J-shape (mono I/J) was also most affected by reduced visibility at the highest level of complexity, indicating that it was more prone to causing users to take their eyes off the road.
Addendum: Cardoso, J., et al. The Influence of Shape on the Output Potential of ZnO Nanostructures: Sensitivity to Parallel versus Perpendicular Forces. Nanomaterials 2018, 8, 354
Authors: Cardoso José , Oliveira Filipe F. , Proenca Mariana P. , Ventura João
Published: June 2020

The authors wish to add the following information to the acknowledgements section of their paper published in Nanomaterials [...]
Dispersibility and Size Control of Silver Nanoparticles with Anti-Algal Potential Based on Coupling Effects of Polyvinylpyrrolidone and Sodium Tripolyphosphate
Authors: Wang Mingshuai , Li Haibo , Li Yinghua , Mo Fan , Li Zhe , Chai Rui , Wang Hongxuan
Published: June 2020

In nearly all the cases of biotoxicity studies of silver nanoparticles (AgNPs), AgNPs used often have general dispersibility and wide size distribution, which may inevitably generate imprecise results. Herein, a kind of synthesis method by coupling effects of polyvinylpyrrolidone (PVP) and sodium tripolyphosphate (STPP) was proposed, in order to prepare AgNPs with better dispersibility and a stable size. Based on this, the preparation mechanism of AgNPs and the potential anti-algae toxicity were analyzed. UV-vis analysis showed that the particle size distribution of AgNPs prepared by co-protective agents was more uniform. X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) were used to confirm that the obtained nano silver was of a high purity and stable size (~30 nm in diameter). Zeta potential and Fourier transform infrared spectroscopy (FTIR) analysis results indicated the synthesis mechanism of AgNPs by co-protective agents, more precisely, PVP limited the polynegative effect and prevented the linear induction of P3O105− produced by STPP during the growth of silver nuclei. Subsequently, Chlorella and Scenedesmus obliquus were utilized to test the toxicity of AgNPs, confirming that AgNPs synthesized through co-protective agents have potential inhibitory ability on algae, but not severe. This study provides a basic theory for the induction of synthetic AgNPs by various factors in the natural environment and a scientific reference for the environmental risk assessment.
A Study of a PID Controller Used in a Micro-Electrical Discharge Machining System to Prepare TiO2 Nanocolloids
Authors: Tseng Kuo-Hsiung , Lin Yur-Shan , Chang Chaur-Yang , Chung Meng-Yun
Published: June 2020

This study developed a micro-electrical discharge machining (micro-EDM) system for producing TiO2 nanocolloids. When a proportional–integral–derivative controller designed using the Ziegler–Nichols method was adopted to control the interelectrode gap, TiO2 nanocolloids were obtained from spark discharges generated between two titanium wires immersed in deionized water. For a pulse on time–off time of 40–40 μs and a colloid production time of 100 min, TiO2 nanocolloids were produced that had an absorbance of 1.511 at a wavelength of 245 nm and a ζ potential of −47.2 mV. They had an average particle diameter of 137.2 nm, and 64.2% of particles were smaller than 91.28 nm. The minimum particles were spherical. The characteristics of colloids confirmed that the micro-EDM system can produce TiO2 nanocolloids with excellent suspension stability. The colloid production method proposed in this study has the advantages of low equipment cost and no dust diffusion in the process environment. These advantages can improve the competitiveness of the electric spark discharge method for high-quality TiO2 nanoparticle production. The colloids produced in this study did not contain elements other than titanium and oxygen, and they may prevent secondary environmental pollution.
Aero-Ga2O3 Nanomaterial Electromagnetically Transparent from Microwaves to Terahertz for Internet of Things Applications
Authors: Braniste Tudor , Dragoman Mircea , Zhukov Sergey , Aldrigo Martino , Ciobanu Vladimir , Iordanescu Sergiu , Alyabyeva Liudmila , Fumagalli Francesco , Ceccone Giacomo , Raevschi Simion , Schütt Fabian , Adelung Rainer , Colpo Pascal , Gorshunov Boris , Tiginyanu Ion
Published: June 2020

In this paper, fabrication of a new material is reported, the so-called Aero-Ga2O3 or Aerogallox, which represents an ultra-porous and ultra-lightweight three-dimensional architecture made from interconnected microtubes of gallium oxide with nanometer thin walls. The material is fabricated using epitaxial growth of an ultrathin layer of gallium nitride on zinc oxide microtetrapods followed by decomposition of sacrificial ZnO and oxidation of GaN which according to the results of X-ray diffraction (XRD) and X-ray photoemission spectroscopy (XPS) characterizations, is transformed gradually in β-Ga2O3 with almost stoichiometric composition. The investigations show that the developed ultra-porous Aerogallox exhibits extremely low reflectivity and high transmissivity in an ultrabroadband electromagnetic spectrum ranging from X-band (8–12 GHz) to several terahertz which opens possibilities for quite new applications of gallium oxide, previously not anticipated.
Remarkable Physical and Thermal Properties of Hydrothermal Carbonized Nanoscale Cellulose Observed from Citric Acid Catalysis and Acetone Rinsing
Authors: Faradilla RH Fitri , Lucia Lucian , Hakovirta Marko
Published: June 2020

Citric acid (CA) was used for the hydrothermal carbonization (HTC) of cellulose nanofiber and found to exert remarkable effects on the chemistry and physical aspects of the product distribution. More specifically, the morphology, yield, elemental and proximate composition, chemical functional groups, thermal properties and surface properties of the resultant hydrochars were studied extensively. The morphological properties of the final char were the singularly most surprising and unique finding of this study. The cellulose nanofiber hydrochars were contrasted to hydrochars from bleached softwood pulp, having a similar composition with the former, to pinpoint the role of nano-dimensions. Without the presence of CA, the pulp hydrochar lacked several of the spherical dimensions shown in the nanocellulose; however, and unexpectedly, the presence of CA caused a homogenization of the final product distribution for both samples. Finally, thermally stable and high surface area hydrochars were obtained when the hydrochar was rinsed with acetone.
Hybrid Sol–Gel Silica Coatings Containing Graphene Nanosheets for Improving the Corrosion Protection of AA2024-T3
Authors: Afsharimani Nasima , Durán Alicia , Galusek Dušan , Castro Yolanda
Published: June 2020

In the present work, nanostructured graphene nanosheets were added to hybrid silica sols and deposited on aluminium alloy A2024-T3 to study the effect on the corrosion behaviour. Sols were prepared using tetraethyl-orthosilicate (TEOS), 3-glycidoxypropyl-trimethoxysilane (GPTMS) and a colloidal silica suspension (LUDOX) as silica precursors with adding chemically modified graphene nanosheets (GN-chem). The graphene nanosheets were modified through a straightforward and simple hydrothermal approach and then, dispersed into a silica sol (SiO2/GN-chem). ATR-FTIR was used to optimize the silica sol–gel synthesis and to confirm the cross-linking of the silica network. The corrosion behaviour of the SiO2/GN-chem coatings was also analysed by electrochemical measurement (potentiodynamic polarization) in 0.05 M NaCl solution. The results showed that the incorporation of modified graphene nanosheets into hybrid silica sol–gel coatings affected the corrosion properties of the substrates. An improvement in the corrosion resistance was observed likely due to the enhanced barrier property and hydrophobic behaviour obtained by incorporation of GN-chem and colloidal silica nanoparticles.
Investigation of Magnetic Circular Dichroism Spectra of Semiconductor Quantum Rods and Quantum Dot-in-Rods
Authors: Safin Farrukh , Maslov Vladimir , Gromova Yulia , Korsakov Ivan , Kolesova Ekaterina , Dubavik Aliaksei , Cherevkov Sergei , Gun’ko Yurii K.
Published: June 2020

Anisotropic quantum nanostructures have attracted a lot of attention due to their unique properties and a range of potential applications. Magnetic circular dichroism (MCD) spectra of semiconductor CdSe/ZnS Quantum Rods and CdSe/CdS Dot-in-Rods have been studied. Positions of four electronic transitions were determined by data fitting. MCD spectra were analyzed in the A and B terms, which characterize the splitting and mixing of states. Effective values of A and B terms were determined for each transition. A relatively high value of the B term is noted, which is most likely associated with the anisotropy of quantum rods.
Radioactive Labeling of Milk-Derived Exosomes with 99mTc and In Vivo Tracking by SPECT Imaging
Authors: González María Isabel , Martín-Duque Pilar , Desco Manuel , Salinas Beatriz
Published: June 2020

Over the last decade, exosomes from diverse biological sources have been proposed as new natural platforms in drug delivery. Translation of these nanometric tools to clinical practice requires deep knowledge of their pharmacokinetic properties and biodistribution. The pharmacokinetic properties of exosomes are sometimes evaluated using biochemical and histological techniques that are considerably invasive. As an alternative, we present radiochemical labeling of milk-derived exosomes based on reduced 99mTc (IV) without modifying biological and physicochemical properties. This approach enables longitudinal tracking of natural exosomes by non-invasive single photon emission computed tomography (SPECT) imaging and the evaluation of their pharmacokinetic properties according to the route of administration.
Switchable Electromagnetically Induced Transparency with Toroidal Mode in a Graphene-Loaded All-Dielectric Metasurface
Authors: Sun Guanghou , Peng Sheng , Zhang Xuejin , Zhu Yongyuan
Published: June 2020

Active photonics based on graphene has attracted wide attention for developing tunable and compact optical devices with excellent performances. In this paper, the dynamic manipulation of electromagnetically induced transparency (EIT) with high quality factors (Q-factors) is realized in the optical telecommunication range via the graphene-loaded all-dielectric metasurface. The all-dielectric metasurface is composed of split Si nanocuboids, and high Q-factor EIT resonance stems from the destructive interference between the toroidal dipole resonance and the magnetic dipole resonance. As graphene is integrated on the all-dielectric metasurface, the modulation of the EIT window is realized by tuning the Fermi level of graphene, engendering an appreciable modulation depth of 88%. Moreover, the group velocity can be tuned from c/1120 to c/3390. Our proposed metasurface has the potential for optical filters, modulators, and switches.
Functionalization of Single and Multi-Walled Carbon Nanotubes with Polypropylene Glycol Decorated Pyrrole for the Development of Doxorubicin Nano-Conveyors for Cancer Drug Delivery
Authors: Pennetta Chiara , Floresta Giuseppe , Graziano Adriana Carol Eleonora , Cardile Venera , Rubino Lucia , Galimberti Maurizio , Rescifina Antonio , Barbera Vincenzina
Published: June 2020

A recently reported functionalization of single and multi-walled carbon nanotubes, based on a cycloaddition reaction between carbon nanotubes and a pyrrole derived compound, was exploited for the formation of a doxorubicin (DOX) stacked drug delivery system. The obtained supramolecular nano-conveyors were characterized by wide-angle X-ray diffraction (WAXD), thermogravimetric analysis (TGA), high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared (FT-IR) spectroscopy. The supramolecular interactions were studied by molecular dynamics simulations and by monitoring the emission and the absorption spectra of DOX. Biological studies revealed that two of the synthesized nano-vectors are effectively able to get the drug into the studied cell lines and also to enhance the cell mortality of DOX at a much lower effective dose. This work reports the facile functionalization of carbon nanotubes exploiting the “pyrrole methodology” for the development of novel technological carbon-based drug delivery systems.
Nanoporous Microsponge Particles (NMP) of Polysaccharides as Universal Carriers for Biomolecules Delivery
Authors: Caso Maria Federica , Carotenuto Felicia , Di Nardo Paolo , Migliore Alberto , Aguilera Ana , Lopez Cruz Matilde , Venanzi Mariano , Cavalieri Francesca , Rinaldi Antonio
Published: June 2020

Different polysaccharides—namely dextran, carboxymethyl dextran, alginate, and hyaluronic acid—were compared for the synthesis of nanoporous microsponges particles (NMPs) obtained from a one-pot self-precipitation/cross-linking process. The morphologies and sizes of the NMPs were evaluated comparatively with respect to polymer-to-polymer and cross-linker solvents (water-based vs. DMSO). We found that the radial distribution of the polymer in the near-spherical NMPs was found to peak either at the core or in the corona of the particle, depending both on the specific polymer or the solvent used for the formation of NMPs. The NMP porosity and the swelling capability were evaluated via scanning electron microscopy (SEM). The degradation study indicated that after 10 h incubation with a reducing agent, approximately 80% of the NMPs were disassembled into soluble polysaccharide chains. The adsorption and release capacity of each type of NMP were evaluated using fluorescently labeled bovine serum albumin and lysozyme as model proteins, highlighting a release time typically much longer than the corresponding adsorption time. The dependence of the adsorption-release performance on pH was demonstrated as well. Confocal microscopy images allowed us to probe the different distribution of labeled proteins inside the NMP. The safety and non-cytotoxicity of NMPs were evaluated after incubation with fibroblast 3T3 cells and showed that all types of NMPs did not adversely affect the cell viability for concentrations up to 2.25 μg/mL and an exposure time up to 120 h. Confocal microscopy imaging revealed also the effective interaction between NMPs and fibroblast 3T3 cells. Overall, this study describes a rapid, versatile, and facile approach for preparing a universal non-toxic, nanoporous carrier for protein delivery under physiological conditions.
Superparamagnetic Iron Oxide Nanoparticles Modified with Silica Layers as Potential Agents for Lung Cancer Treatment
Authors: Reczyńska Katarzyna , Marszałek Marta , Zarzycki Arkadiusz , Reczyński Witold , Kornaus Kamil , Pamuła Elżbieta , Chrzanowski Wojciech
Published: June 2020

Superparamagnetic iron oxide nanoparticles (SPIONs) are promising drug delivery carriers and hyperthermia agents for the treatment of cancer. However, to ensure their safety in vivo, SPIONs must be modified in order to prevent unwanted iron release. Thus, SPIONs were coated with silica layers of different morphologies: non-porous (@SiO2), mesoporous (@mSiO2) or with a combination of non-porous and mesoporous layers (@SiO2@mSiO2) deposited via a sol–gel method. The presence of SiO2 drastically changed the surface properties of the nanoparticles. The zeta potential changed from 19.6 ± 0.8 mV for SPIONs to −26.1 ± 0.1 mV for SPION@mSiO2. The Brunauer–Emmett–Teller (BET) surface area increased from 7.54 ± 0.02 m2/g for SPIONs to 101.3 ± 2.8 m2/g for SPION@mSiO2. All types of coatings significantly decreased iron release (at least 10 fold as compared to unmodified SPIONs). SPIONs and SPION@mSiO2 were tested in vitro in contact with human lung epithelial cells (A549 and BEAS-2B). Both nanoparticle types were cytocompatible, although some delay in proliferation was observed for BEAS-2B cells as compared to A549 cells, which was correlated with increased cell velocity and nanoparticles uptake.