bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Palliative Care on Mitigating Pain and Its Associated Effects in Determining Quality of Life among Colon Cancer Outpatients
Authors: Macharia John M. , Raposa Bence L. , Sipos Dávid , Melczer Csaba , Toth Zoltan , Káposztás Zsolt
Published: November 2023

Pain continues to be a significant problem for cancer patients, and the impact of a population-based strategy on their experiences is not completely understood. Our study aimed to determine the impact of palliative care on mitigating pain and its associated effects in determining the quality of life (QoL) among colon cancer outpatients. Six collection databases were used to perform a structured systematic review of the available literature, considering all papers published between the year 2000 and February 2023. PRISMA guidelines were adopted in our study, and a total of 9792 papers were evaluated. However, only 126 articles met the inclusion criteria. A precise diagnosis of disruptive colorectal cancer (CRC) pain disorders among patients under palliative care is necessary to mitigate it and its associated effects, enhance health, promote life expectancy, increase therapeutic responsiveness, and decrease comorbidity complications. Physical activities, the use of validated pain assessment tools, remote outpatient education and monitoring, chemotherapeutic pain reduction strategies, music and massage therapies, and bridging social isolation gaps are essential in enhancing QoL. We recommend and place a strong emphasis on the adoption of online training/or coaching programs and the integration of formal and informal palliative care systems for maximum QoL benefits among CRC outpatients.
Reply to Moreno et al. Comment on “Sallam, M. ChatGPT Utility in Healthcare Education, Research, and Practice: Systematic Review on the Promising Perspectives and Valid Concerns. Healthcare 2023, 11, 887”
Authors: Sallam Malik
Published: November 2023

I would like to thank the authors for their commentary on the publication “ChatGPT Utility in Healthcare Education, Research, and Practice: Systematic Review on the Promising Perspectives and Valid Concerns” [...]
Absenteeism Costs Due to COVID-19 and Their Predictors in Non-Hospitalized Patients in Sweden: A Poisson Regression Analysis
Authors: Kisiel Marta A. , Lee Seika , Janols Helena , Faramarzi Ahmad
Published: November 2023

Background: This study aimed to estimate absenteeism costs and identify their predictors in non-hospitalized patients in Sweden. Methods: This cross-sectional study’s data were derived from the longitudinal project conducted at Uppsala University Hospital. The mean absenteeism costs due to COVID-19 were calculated using the human capital approach, and a Poisson regression analysis was employed to determine predictors of these costs. Results: The findings showed that the average absenteeism cost due to COVID-19 was USD 1907.1, compared to USD 919.4 before the pandemic (p < 0.001). Notably, the average absenteeism cost for females was significantly higher due to COVID-19 compared to before the pandemic (USD 1973.5 vs. USD 756.3, p = 0.001). Patients who had not fully recovered at the 12-month follow-up exhibited significantly higher costs than those without symptoms at that point (USD 3389.7 vs. USD 546.7, p < 0.001). The Poisson regression revealed that several socioeconomic factors, including age, marital status, country of birth, educational level, smoking status, BMI, and occupation, along with COVID-19-related factors such as severity at onset, pandemic wave, persistent symptoms at the follow-up, and newly introduced treatment for depression after the infection, were significant predictors of the absenteeism costs. Conclusions: Our study reveals that the mean absenteeism costs due to COVID-19 doubled compared to the year preceding the pandemic. This information is invaluable for decision-makers and contributes to a better understanding of the economic aspects of COVID-19.
Landscape Sensitivity Assessment of Historic Districts Using a GIS-Based Method: A Case Study of Beishan Street in Hangzhou, China
Authors: Yang Xueyan , Shen Jie
Published: November 2023

Historic districts may be damaged during urban renewal. Landscape sensitivity can be used as a method to judge the ability of a landscape to resist change. This study proposes an improved method for assessing landscape sensitivity based on a geographic information system (GIS) according to the characteristics of historic districts. Based on a previous method, this study adds POI big data for comprehensive evaluation and uses objective criteria importance through intercriteria correlation (CRITIC) statistics instead of subjective methods to determine the weights. The assessment framework uses ecological, visual, and cultural sensitivity as primary criteria, which are further defined by several sub-criteria. The Beishan Street Historic District in Hangzhou, China, is used as a case study, and the results of the assessment are shown in the form of sensitivity maps. The results show that the maps can identify buildings in areas of high sensitivity and provide objective indicators for future conservation. Based on the sensitivity maps, this study innovatively used correlation analysis to reveal important interrelationships between ecological, visual, and cultural sensitivity. Assessment factors such as land use type need to be prioritized because they are more closely linked to other factors.
Identification and Functional Verification of CITED2 Gene Promoter Region in Patients with Patent Ductus Arteriosus
Authors: Chen Zhuo , Chen Huan-Xin , Hou Hai-Tao , Yin Xiu-Yun , Yang Qin , He Guo-Wei
Published: November 2023

Patent ductus arteriosus (PDA) is a common congenital heart disease. CITED2 plays an important role in the development of the heart, and genetic variants in its coding region are significantly associated with cardiac malformations. However, the role of variants in the promoter region of CITED2 in the development of PDA remains unclear. We extracted the peripheral blood of 646 subjects (including 353 PDA patients and 293 unrelated healthy controls) for sequencing. We identified 13 promoter variants of the CITED2 gene (including 2 novel heterozygous variants). Of the 13 variants, 10 were found only in PDA patients. In mouse cardiomyocytes (HL-1) and rat cardiac myocytes (RCM), the transcriptional activity of the CITED2 gene promoter was significantly changed by the variants (p < 0.05). The results of the experiments of electrophoretic mobility indicated that these variants may affect the transcription of the CITED2 gene by influencing the binding ability of transcription factors. These results, combined with the JASPAR database analysis, showed that the destruction/production of transcription factor binding sites due to the variants in the promoter region of the CITED2 gene may directly or indirectly affect the binding ability of transcription factors. Our results suggest for the first time that variants at the CITED2 promoter region may cause low expression of CITED2 protein related to the formation of PDA.
Tobacco Leaf-Surface Extracts: Antimicrobial Potential against Phytopathogenic Fungi and In Vitro Culture Bacterial Contaminants
Authors: Capdesuñer Yanelis , Linares Claudia , Schöne Jochen , El-Hasan Abbas , Vögele Ralf , Yanes-Paz Ermis , Ortega-Delgado Eduardo , Martínez-Montero Marcos Edel , Rodríguez Erinelvis , Quiñones-Gálvez Janet
Published: November 2023

The excessive use of synthetic chemicals in agriculture demands sustainable alternatives to combat crop-affecting microorganisms. Plant-derived secondary metabolites have garnered attention as promising candidates with antimicrobial properties. This study investigates the antimicrobial potential of tobacco plants, specifically non-commercial accessions Nic 1015 (“TI 1341”) and BHmN, recognized for their rich bioactive compounds. Our objectives encompassed the extraction of leaf surface compounds and the assessment of their in vitro antimicrobial activity against crop-damaging microorganisms. Ethanol-based extracts, abundant in diterpenes, were meticulously analyzed. Notably, BHmN contained cis-abienol, while both accessions featured α-CBT diol, as confirmed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). TLC-Bioautography and microdilution assays unveiled substantial antifungal activity. The growth inhibition percentages correlated with extract concentrations, highlighting the pivotal role of diterpenes. These extracts exhibited pronounced efficacy against Rhizoctonia solani and Stemphylium solani but displayed relatively weaker activity against Sarocladium oryzae. Notably, Nic 1015 extract demonstrated remarkable antifungal activity at a minimal concentration of 78 µg·mL−1, while cis-abienol and sclareol inhibited the growth of Fusarium graminearum and Alternaria alternata. Additionally, the extracts demonstrated in vitro antibacterial activity against common plant culture contaminants, Bacillus licheniformis and Stenotrophomonas maltophilia. In conclusion, the findings underscore the potential of these extracts as effective tools for controlling pathogenic fungi and bacterial contaminants in plant in vitro cultures. Harnessing plant-derived secondary metabolites, especially those from tobacco leaf surface, presents a sustainable and eco-friendly strategy to mitigate the detrimental impact of microorganisms on agricultural crops, promising a greener alternative to synthetic chemical products.
Pharmaceutical Interventions for Inpatients with Liver Cirrhosis and Liver Transplantation: A Systematic Review of Experimental Studies
Authors: Jibai Nagham , Koch Alexander , Ulmer Tom Florian , Erdmann Pia , Koeck Joachim Andreas , Eisert Albrecht
Published: November 2023

Liver cirrhosis, which is considered one of the leading causes of death in the world, can lead to severe complications, and is often followed by a liver transplantation. These patients take an average of nine medications daily. If not managed adequately, it can be accompanied by serious drug-related problems. To reduce this risk, a clinical pharmacist may be included as part of the healthcare team to optimize medication therapy in this population. This study aimed to systematically identify the pharmaceutical interventions which reduced drug-related problems and improved medication therapy for adult hospitalized liver cirrhotic and liver transplant patients when compared to standard care. Three databases (PubMed, Embase, and CENTRAL) were systematically searched from the inception of each database to 25 October 2023, and interventional studies in the English language were included. The risk of bias was assessed according to RoB-I for the UBA study and RoB2 for the identified RCT. The detected interventions to reduce drug-related problems in liver cirrhotic and liver transplant patients were extracted and classified according to a “Hierarchy of Controls” model. Two studies from Germany and the USA met our inclusion criteria, respectively. In these studies, we identified two interventions that included education, expert consultation, and the monitoring of the immunosuppressive medications serum level. The main objective of the two included studies was improving patients’ compliance through adherence. These pharmaceutical interventions identified were classified as administrative controls, which is one of the lowest levels in the “Hierarchy of Controls” with which to address a potential risk. Pharmaceutical interventions to optimize medication therapy were found to be rare in the examined population, and were limited to “administrative controls”. These interventions were limited to transplant patients’ education and the monitoring of the immunosuppressive medication serum levels. No interventional studies were found to have investigated pharmaceutical interventions in patients with liver cirrhosis. Especially regarding this patient group, future studies to reduce DRPs using pharmaceutical interventions are needed. This study received no external funding and its PROSPERO registration number is CRD42022309122.
Otorhinolaryngological Manifestations and Esophageal Disorders in Celiac Disease: A Narrative Review
Authors: Wieser Herbert , Ciacci Carolina , Gizzi Carolina , Santonicola Antonella
Published: November 2023

Celiac disease (CeD) is a chronic gluten-sensitive immune-mediated enteropathy characterized by numerous intestinal and extra-intestinal signs and symptoms. Among extra-intestinal manifestations, otorhinolaryngological (ORL) complaints in CeD are relatively rare and their relation to CeD is frequently overlooked by physicians. Recent studies underlined that the prevalence of recurrent aphthous stomatitis, aphthous ulcers, geographic tongue, and xerostomia was significantly increased in CeD patients compared with healthy individuals. However, data about the other oral manifestations of CeD, such as atrophic glossitis, glossodynia, angular cheilitis, and salivary abnormalities, are scanty. Further ORL conditions associated with CeD include sensorineural hearing loss, nasal abnormalities, and obstructive sleep apnea. Moreover, several esophageal disorders such as gastroesophageal reflux disease and eosinophilic esophagitis have been associated with CeD. The pathophysiological link between both ORL and esophageal manifestations and CeD might be further investigated. In addition, also the role of gluten-free diet in improving these conditions is largely unclear. Certainly, otorhinolaryngologists can play an important role in identifying people with unrecognized CeD and may help prevent its long-term complications. The aim of this narrative review is to analyze the latest evidence on the association between CeD and ORL and esophageal manifestations.
Impact of the Body Composition on Knee Osteoarthritis Assessed Using Bioimpedance Analysis
Authors: Jarecki Jaromir , Potoczniak Bartosz , Dziedzic Artur , Małecka-Masalska Teresa , Skrzypek Tomasz , Kazimierczak Waldemar , Skowronek Marcin , Wójciak Magdalena , Dresler Sławomir , Waśko Marcin , Sowa Ireneusz
Published: November 2023

Osteoarthritis (OA) ranks among the most prevalent inflammatory diseases affecting the musculoskeletal system and is a leading cause of disability globally, impacting approximately 250 million individuals. This study aimed to assess the relationship between the severity of knee osteoarthritis (KOA) and body composition in postmenopausal women using bioimpedance analysis (BIA). The study included 58 postmenopausal females who were candidates for total knee arthroplasty. The control group consisted of 25 postmenopausal individuals with no degenerative knee joint changes. The anthropometric analysis encompassed the body mass index (BMI), mid-arm and mid-thigh circumferences (MAC and MTC), and triceps skinfold thickness (TSF). Functional performance was evaluated using the 30 s sit-to-stand test. During the BIA test, electrical parameters such as membrane potential, electrical resistance, capacitive reactance, impedance, and phase angle were measured. Additionally, body composition parameters, including Total Body Water (TBW), Extracellular Water (ECW), Intracellular Water (ICW), Body Cellular Mass (BCM), Extracellular Mass (ECM), Fat-Free Mass (FFM), and Fat Mass (FM), were examined. The study did not find any statistically significant differences in the electrical parameters between the control (0–1 grade on the K–L scale) and study groups (3–4 grade on the K–L scale). However, statistically significant differences were observed in BMI, fat mass (FM), arm circumference, triceps skinfold thickness, and sit-to-stand test results between the analyzed groups. In conclusion, the association between overweight and obesity with KOA in postmenopausal women appears to be primarily related to the level of adipose tissue and its metabolic activity.
The Diagnostic Utility of Artificial Intelligence-Guided Computed Tomography-Based Severity Scores for Predicting Short-Term Clinical Outcomes in Adults with COVID-19 Pneumonia
Authors: Atceken Zeynep , Celik Yeliz , Atasoy Cetin , Peker Yüksel
Published: November 2023

Chest computed tomography (CT) imaging with the use of an artificial intelligence (AI) analysis program has been helpful for the rapid evaluation of large numbers of patients during the COVID-19 pandemic. We have previously demonstrated that adults with COVID-19 infection with high-risk obstructive sleep apnea (OSA) have poorer clinical outcomes than COVID-19 patients with low-risk OSA. In the current secondary analysis, we evaluated the association of AI-guided CT-based severity scores (SSs) with short-term outcomes in the same cohort. In total, 221 patients (mean age of 52.6 ± 15.6 years, 59% men) with eligible chest CT images from March to May 2020 were included. The AI program scanned the CT images in 3D, and the algorithm measured volumes of lobes and lungs as well as high-opacity areas, including ground glass and consolidation. An SS was defined as the ratio of the volume of high-opacity areas to that of the total lung volume. The primary outcome was the need for supplemental oxygen and hospitalization over 28 days. A receiver operating characteristic (ROC) curve analysis of the association between an SS and the need for supplemental oxygen revealed a cut-off score of 2.65 on the CT images, with a sensitivity of 81% and a specificity of 56%. In a multivariate logistic regression model, an SS > 2.65 predicted the need for supplemental oxygen, with an odds ratio (OR) of 3.98 (95% confidence interval (CI) 1.80–8.79; p < 0.001), and hospitalization, with an OR of 2.40 (95% CI 1.23–4.71; p = 0.011), adjusted for age, sex, body mass index, diabetes, hypertension, and coronary artery disease. We conclude that AI-guided CT-based SSs can be used for predicting the need for supplemental oxygen and hospitalization in patients with COVID-19 pneumonia.
Early Stabilization Does Not Increase Complication Rates in Acetabular Fractures of the Elderly: A Retrospective Analysis from the German Pelvis Registry
Authors: Regenbogen Stephan , Leister Iris , Trulson Alexander , Wenzel Lisa , Friederichs Jan , Stuby Fabian M. , Höch Andreas , Beck Markus ,   Working Group on Pelvic Fractures of the German Trauma Society
Published: November 2023

Background: The incidence of acetabular fractures in geriatric patients has increased. Although there are strong data supporting the early operative treatment of hip fractures in geriatric patients, the optimal timing for acetabular fractures remains unclear and for several reasons, delayed treatment after trauma is common. Methods: A retrospective analysis of the German Pelvis Registry between 2008 and 2017 was performed. Ultimately, 665 patients with fractures of the anterior column or anterior column and posterior hemitransverse were enrolled. Patients above and below 65 years of age with these fracture types were analyzed regarding surgery day (within 48 hours, between 2 and 4 days, after 4 days), complication rate, reduction quality, and hospital stay. Results: The complication rate of the geriatric group was twice as high as that of younger patients; however, this finding was independent of the timing of surgery. Reduction quality and hospital stay were independent of surgical timing. Conclusions: In contrast to other fracture types, such as proximal femur fractures, the timing of surgery for acetabular fractures does not have a significant impact on the patient’s outcome. The optimal time for surgery cannot be determined using the current data. However, as expected, there is a significantly higher risk for postoperative complications in the geriatric population.
Primary Biliary Cholangitis (PBC)-Autoimmune Hepatitis (AIH) Variant Syndrome: Clinical Features, Response to Therapy and Long-Term Outcome
Authors: Graf Markus , Lange Christian M. , Langer Mona M. , Schattenberg Jörn M. , Seessle Jessica , Dietz Julia , Vermehren Annika , Michael Florian A. , Mondorf Antonia , Zeuzem Stefan , Pathil Anita , Graf Christiana
Published: November 2023

Introduction: Standardization of diagnostic criteria of autoimmune hepatitis (AIH) and primary biliary cholangitis (PBC) variant syndrome (AIH-PBC VS) has not been achieved so far and evidence-based recommendations for monitoring and treatment of the disease are still lacking. Our study aimed to assess the prevalence, biochemical, and serological features, as well as the clinical course, of VS. Methods: We performed a retrospective study including all patients with VS between 1999 and 2020 in four German centers. Data on demographic parameters, biochemical and serological tests, treatment, and outcome were collected. Results: Of 90 patients (3.1%) meeting Paris criteria for VS diagnosis, 65.6% showed AIH and PBC histological features, while biochemical Paris criteria were observed comparatively rarely. Further antibodies, which were not part of the diagnostic criteria of VS, were found in a subgroup of patients with available data (ACA: 30.0%; anti-CENP-A: 25.0%; anti-CENP-B: 33.3%; anti-SP100: 21.4%). Biochemical response was more frequently observed in patients treated with a combined therapy of ursodeoxycholic acid (UDCA) and immunosuppression (IS). Liver cirrhosis was detected in 31 patients (34.4%) and 25 patients (27.8%) developed clinical manifestations of portal hypertension. Conclusions: Biochemical Paris criteria of VS were rarely detected, thus implying that these cut-off values should be redefined. Regarding pharmacological treatment, combined therapy of UDCA and IS appeared to be more effective than monotherapy with UDCA.
Effect of Blade Size on the First-Pass Success Rate of Endotracheal Intubation Using the C-MAC Video Laryngoscope
Authors: Park Jeongyong , Park Goeun , Kim Da Seul , Kim Minha , Heo Sejin , Jeong Daun , Chang Hansol , Lee Se Uk , Choi Goosang , Lee Gun Tak , Shin Tae Gun , Park Jong Eun , Hwang Sung Yeon
Published: November 2023

We sought to determine whether blade size influences the first-pass success (FPS) rate when performing endotracheal intubation (ETI) with a C-MAC video laryngoscope (VL) in emergency department (ED) patients. This single-center, retrospective, observational study was conducted between August 2016 and July 2022. A total of 1467 patients was divided into two categories based on the blade size used during the first ETI attempt: blade-3 (n = 365) and blade-4 groups (n = 1102). The primary outcome was the FPS rate. The secondary outcomes included the glottic view, multiple attempt rate, and ETI-related complications. We used propensity score matching to reduce the potential confounders between the two groups. Among these, 363 pairs of matched propensity scores were generated. The FPS rate did not differ between the blade-3 (84.8%) and blade-4 groups (87.3%) in the matched cohort (p = 0.335). The multiple attempt rate did not differ significantly between groups (p = 0.289) and was 3.9% and 2.5% in the blade-3 and blade-4 groups, respectively. The difficult glottic view (11.3 vs. 6.9%, p = 0.039) and complication rates (15.4% vs. 10.5%, p = 0.047) were significantly higher in the blade-3 group than in the blade-4 group. The FPS rates of ETI with the blade-3 and blade-4 groups in adult patients in the ED did not differ significantly.
Bioprosthetic Valves for Lifetime Management of Aortic Stenosis: Pearls and Pitfalls
Authors: Mylonas Konstantinos S. , Angouras Dimitrios C.
Published: November 2023

This review explores the use of bioprosthetic valves for the lifetime management of patients with aortic stenosis, considering recent advancements in surgical (SAV) and transcatheter bioprostheses (TAV). We examine the strengths and challenges of each approach and their long-term implications. We highlight differences among surgical bioprostheses regarding durability and consider novel surgical valves such as the Inspiris Resilia, Intuity rapid deployment, and Perceval sutureless bioprostheses. The impact of hemodynamics on the performance and durability of these prostheses is discussed, as well as the benefits and considerations of aortic root enlargement during Surgical Aortic Valve Replacement (SAVR). Alternative surgical methods like the Ross procedure and the Ozaki technique are also considered. Addressing bioprosthesis failure, we compare TAV-in-SAV with redo SAVR. Challenges with TAVR, such as TAV explantation and considerations for coronary circulation, are outlined. Finally, we explore the potential challenges and limitations of several clinical strategies, including the TAVR-first approach, in the context of aortic stenosis lifetime management. This concise review provides a snapshot of the current landscape in aortic bioprostheses for physicians and surgeons.
Further Association of Germline CHEK2 Loss-of-Function Variants with Testicular Germ Cell Tumors
Authors: Kirchner Kira , Seidel Christoph , Paulsen Finn-Ole , Sievers Bianca , Bokemeyer Carsten , Lessel Davor
Published: November 2023

Testicular germ cell tumors (TGCTs) represent the most frequent malignancy in young adult men and have one the highest heritability rates among all cancers. A recent multicenter case–control study identified CHEK2 as the first moderate-penetrance TGCT predisposition gene. Here, we analyzed CHEK2 in 129 TGCT cases unselected for age of onset, histology, clinical outcome, and family history of any cancer, and the frequency of identified variants was compared to findings in 27,173 ancestry-matched cancer-free men. We identified four TGCT cases harboring a P/LP variant in CHEK2 (4/129, 3.10%), which reached statistical significance (p = 0.0191; odds ratio (OR), 4.06; 95% CI, 1.59–10.54) as compared to the control group. Cases with P/LP variants in CHEK2 developed TGCT almost 6 years earlier than individuals with CHEK2 wild-type alleles (5.67 years; 29.5 vs. 35.17). No association was found between CHEK2 status and further clinical and histopathological characteristics, including histological subtypes, the occurrence of aggressive TGCT, family history of TGCT, and family history of any cancer. In addition, we found significant enrichment for the low-penetrance CHEK2 variant p.Ile157Thr (p = 0.0259; odds ratio (OR), 3.69; 95% CI, 1.45–9.55). Thus, we provide further independent evidence of CHEK2 being a moderate-penetrance TGCT predisposition gene.
Emotional Distress Caused by the Measures Taken in Assisted Reproductive Treatments during the COVID-19 Confinement in Spain
Authors: Correa Rancel Marta , Sosa Comino Elena , Leon-Larios Fatima , Suárez Hernández Yaiza , Carballo Lorenzo Janet , Gomez-Baya Diego , Baez Quintana Delia
Published: November 2023

During the pandemic, assisted reproductive treatments suffered from major disruptions in their terms due to the restrictions imposed. The objective of this study is to evaluate the level of anxiety of women whose treatments were either suspended or delayed. Methods: Descriptive cross-sectional study conducted between April and May 2020. The State-Trait Anxiety Inventory was applied by telephone in a Spanish adapted version. The research also included social, personal, and work aspects which may be involved in the challenging situation. Results: A total of 115 patients participated in the study (73.7%). Women showed a mean in trait anxiety of 17.79 (SD = 8.80) and a mean in state anxiety of 19.95 (SD = 9.08). Neither the type of treatment nor the time of infertility were predictors of trait anxiety or state anxiety. Greater age pressure and more worry were associated to greater trait and state anxiety (p < 0.001). The most common emotional reactions to discontinuation of fertility treatments were sadness and anxiety. Conclusions: Discontinuation of fertility treatments due to confinement restrictions had a negative impact on the mental health of women who were following a process of assisted reproduction treatment, increasing their levels of emotional distress and anxiety.
Efficacy of Low-Level Laser Therapy for Oral Mucositis in Hematologic Patients Undergoing Transplantation: A Single-Arm Prospective Study
Authors: Nishi Hiromi , Horikoshi Susumu , Ohta Kouji , Yoshida Tetsumi , Fukushima Noriyasu , Oshita Kyoko , Munenaga Syuichi , Edahiro Taro , Ureshino Hiroshi , Shigeishi Hideo , Yoshioka Yukio , Konishi Masaru , Ide Noriaki , Ogawa Yuma , Marukawa Rikou , Shintani Tomoaki , Ino Natumi , Kajiya Mikihito , Kakimoto Naoya , Ohge Hiroki , Ichinohe Tatsuo , Kawaguchi Hiroyuki
Published: November 2023

Oral mucositis significantly affects the quality of life in hematologic cancer patients undergoing hematopoietic stem cell transplantation. Despite global evidence supporting the efficacy of low-level laser therapy (LLLT) for mucositis prevention, its clinical adoption in Japan is limited. This study aimed to fill this gap by evaluating the safety and efficacy of LLLT in a Japanese patient population. In a single-group, non-blinded, exploratory trial, we compared 21 LLLT-treated patients against a historical control of 96 patients. The primary endpoint was the incidence of Grade ≥ 2 mucositis, based on NCI-CTCAE ver. 4.0. The LLLT group showed a significantly lower incidence of Grade ≥ 2 mucositis (23.8%) compared to the control group (64.6%) (p = 0.0006). Furthermore, Grade ≥ 2 mucositis correlated with increased oral dryness and longer hospital stays. Our study confirms the efficacy of LLLT in reducing the onset of severe oral mucositis among Japanese hematologic cancer patients, advocating for its clinical introduction as a preventive measure in Japan.
Ecological Change and Livestock Governance in a Peruvian National Park
Authors: Young Kenneth , Alata Eyner , Chimner Rodney , Boone Randall , Bowser Gillian , Bourgeau-Chavez Laura , Fuentealba Beatriz , Gilbert Jessica , Ñaupari Javier , Polk Molly , Resh Sigrid , Turin Cecilia , Zarria-Samanamud Melody
Published: November 2023

While the grazing of livestock has occurred for millennia in the Andes, current sustainability debates center on concerns with co-managing climate change and pastoralism. These discussions have special resonance in places protected by the state for biodiversity, scenery, and sustainable and traditional land uses, such as those found in protected areas and biosphere reserves. For this article, we integrate data from a social-ecological research project on the land use systems that affect high-elevation ecosystems in Peru’s Huascarán National Park, with special emphasis on the wetlands. We used land cover and land use data and insights from interactions with pastoralists to show that (1) wet meadows dominate the lower reaches of the park, while peatlands predominate above 4000 m elevation; (2) wet meadows are most useful for traditional grazing systems, while the peatlands are especially susceptible to trampling by livestock; and (3) there is limited ecological space at the highest elevations for the successful future upward relocation of either land use or potential habitats for species identified as of concern. We explore the implications of these findings for the adaptive strategies of biophysical and social processes in terms of livelihoods and biodiversity in and around a protected area. We conclude that there are many additional opportunities to be explored to inform the management of ecosystem services and provide improvements for the adaptive capacity of communities and park managers.
Impacts of Climate Change on Vegetation in Kenya: Future Projections and Implications for Protected Areas
Authors: Parracciani Cecilia , Buitenwerf Robert , Svenning Jens-Christian
Published: November 2023

Climate change will cause substantial vegetation shifts across the world. Africa may face varying dynamics such as tree decline, savannization, and woody encroachment due to rising temperatures and rainfall changes. This study examines the potential effects of climate change on Kenyan vegetation and vegetation shifts for 2050 and 2100, employing a statistical model to predict vegetation state as driven by environmental variables, including temperature, soil moisture, livestock density, and topography. We evaluate the model by hindcasting it from 2020 to 2000 and then project future vegetation states for 2050 and 2100 under SSP 2–4.5 and SSP 5–8.5. In response to moderate emissions, a notable increase in arid-associated shrubland vegetation (53–58%) is forecasted, leading to the expansion of drylands at the expense of savannas, grasslands, and forests. Under high-emission scenarios, savannas are forecasted to expand (52–65%) at the expense of forested areas. Overall, dense forest cover declines across scenarios, affecting protected areas by promoting increased savanna cover and reducing forest area (40% to 50%). These projected shifts in major vegetation types would likely alter ecosystem functioning and associated services, impacting pastoralists and wildlife and raising biodiversity concerns. Protected areas in Kenya could lose 50% of their forests, highlighting the urgency of climate change mitigation. These findings offer a crucial foundation for future research and action on Kenya’s vegetation.
Understanding Geodiversity for Sustainable Development in the Chinchiná River Basin, Caldas, Colombia
Authors: Arias-Díaz Alejandro , Murcia Hugo , Vallejo-Hincapié Felipe , Németh Károly
Published: November 2023

Geodiversity, comprising both endogenous and exogenous geological processes, plays a crucial role in shaping the structure and functionality of natural systems, alongside its substantial impact on human well-being. However, the often-overlooked interconnection between geodiversity components limits our comprehension of geosystems. In the Chinchiná River Basin (CRB) in Colombia, located in the northern Andes in South America, we established criteria to differentiate geodiversity classes, calculated indices to understand the distribution of geological elements, and discussed systemic relationships. This comprehensive approach lays the foundation for a holistic comprehension of the territory’s structure and functionality. Our findings revealed the convergence in an area of 1052 km2 of 10 rock types, 7 slope ranges, 13 landforms, 5 drainage density features, 610.4 km of faults with 9 kinematic tendencies, 5 soil orders, 5 climate types, a 3328 km surface drainage network with 7 hydrographic orders, 1 underground aquifer, 4 areas with lakes, 2 zones with glaciers, 27 polygenetic and monogenetic volcanoes, and several thermal springs. This discussion explores the implications of various methodologies used to establish the value of the general geodiversity index while also examining the relationships between abiotic elements and their distribution patterns. This forms a fundamental basis for understanding the geosystem services of the basin in terms of regulation, support, and provisioning processes, as well as the culture and knowledge derived from geodiversity. These conceptual elements are indispensable for enhancing the sustainability of a region that is susceptible to the impacts of climate change. Furthermore, they serve as the foundations for the objective’s achievement, as set by the UNESCO Global Geopark project “Volcán del Ruiz”, currently ongoing within the region.
Sediment Carbon Stock in Natural and Transplanted Mangroves in Bahrain, Arabian Gulf
Authors: Naser Humood Abdulla
Published: November 2023

Mangroves in the Arabian Gulf provide several biological, ecological, and environmental services. They are also considered among the largest carbon sinks. However, mangroves along the coastlines of the Arabian Gulf have degraded in recent decades, mainly due to urbanization and coastal development. Therefore, restoration and afforestation programs have been initiated to enhance the services and functions of mangrove ecosystems and as part of national targets to mitigate climate change. Increasing carbon sinks by quadrupling the current areas covered by mangroves through afforestation programs by 2035 is one of the strategies to mitigate climate change in Bahrain. The aim of the present study was to estimate the organic carbon stocks in the sediments of natural and transplanted mangroves in Bahrain. Within the protected areas of Tubli and Arad Bays, sediment samples were taken down to a depth of 70 cm from natural and transplanted mangroves as well as a bare mudflat. The findings of the present study indicated that the total sediment organic carbon concentrations at three sampling sites of natural and transplanted mangroves and the mudflat were 200.54 ± 24.52, 112.36 ± 55.51, and 81.56 ± 8.92 Mg C/ha, respectively. The natural mangroves in Tubli Bay differed considerably from those in Arad Bay (p ≤ 0.001), based on the concentrations of organic carbon in sediments. However, there was a noticeable similarity seen in the organic carbon of the mangroves in Arad Bay that were transplanted 25 years prior and the natural mangroves in Tubli Bay, indicating the importance of a long-term mangrove afforestation strategy to mitigate climate change in the Arabian Gulf.
Site History’s Role in Urban Agriculture: A Case Study in Kisumu, Kenya, and Ouagadougou, Burkina Faso
Authors: Jonkman Nicolette Tamara , Kalbitz Karsten , Bergsma Huig , Jansen Boris
Published: November 2023

Urban agriculture (UA) is a widespread practice often considered low-profit, taking place on marginal lands. This is supported by the lack of quantitative data on UA’s contributions to food security and employment, yet contradicted by prevalence and high participation rates. This case study of six UA sites in Kisumu, Kenya and Ouagadougou, Burkina Faso explores the relationship between prior land use and current management and soil quality. A soil survey is performed determining the soil macronutrient and soil mineral composition. Agricultural management, ownership, and prior land use are investigated through interviews, satellite imagery, and historic publications. Results show three UA sites predating surrounding urban development, and data on soil nutrient content show that sites likely were chosen for their soil. The three younger sites are smaller and less embedded in the local economy, but soil analysis shows medium-rich to rich agricultural soils. We conclude that one cannot assume that UA is practiced on marginalized soils. Consequently, both value attribution to and the sustainable agricultural management of UA soils must be based on their characteristics, such as mineralogy and nutrient status, to prevent valuable soil resources from being lost. Through this, the more accurate value attribution of UA can be achieved, lending weight to the value attributed to UA by local communities.
Examining Relationships between Regional Ecological Risk and Land Use Using the Granger Causality Test Applied to a Mining City, Daye, China
Authors: Guo Kai , He Zhenhao , Liang Xiaojin , Chen Xuanwei , Luo Renbo , Qiu Tianqi , Zhang Kexin
Published: November 2023

Land use changes are an important factor contributing to the increasingly severe deterioration of the ecological environment. Therefore, regional analyses of land use and ecological risk should be developed for the restoration of ecological functions. In this study, a comprehensive ecological risk assessment was constructed on a regional scale and applied to Daye, a traditional mining city in China. Cointegration analyses and Granger causality tests were used to explore the complex relationship between land use and ecological risks in the study area from 2007 to 2021. The results show a long-term and stable relationship between land use changes in different sub-regions and ecological risks, albeit with distinct Granger causality relationships. This research presents the development trend of the relationship between land use change and ecological risks in a mining city, from rapid economic growth to economic restructuring and full-region ecological governance.
Recurrent Somatic Copy Number Alterations and Their Association with Oncogene Expression Levels in High-Grade Ovarian Serous Carcinoma
Authors: Esplen Hillary P. , Yang Richard K. , Kalia Awdhesh , Tang Zhenya , Tang Guilin , Medeiros L. Jeffrey , Toruner Gokce A.
Published: November 2023

Somatic copy number alterations (SCNAs) are frequently observed in high-grade ovarian serous carcinoma (HGOSC). However, their impact on gene expression levels has not been systematically assessed. In this study, we explored the relationship between recurrent SCNA and gene expression using The Cancer Genome Atlas Pan Cancer dataset (OSC, TCGA, PanCancer Atlas) to identify cancer-related genes in HGOSC. We then investigated any association between highly correlated cancer genes and clinicopathological parameters, including age of diagnosis, disease stage, overall survival (OS), and progression-free survival (PFS). A total of 772 genes with recurrent SCNAs were observed. SCNA and mRNA expression levels were highly correlated for 274 genes; 24 genes were classified as a Tier 1 gene in the Cancer Gene Census in the Catalogue of Somatic Mutations in Cancer (CGC-COSMIC). Of these, 11 Tier 1 genes had highly correlated SCNA and mRNA expression levels: TBL1XR1, PIK3CA, UBR5, EIF3E, RAD21, EXT1, RECQL4, KRAS, PRKACA, BRD4, and TPM4. There was no association between gene amplification and disease stage or PFS. EIF3E, RAD21, and EXT1 were more frequently amplified in younger patients, specifically those under the age of 55 years. Patients with tumors carrying PRKACA, BRD4, or TPM4 amplification were associated with a significantly shorter OS. RECQL4 amplification was more frequent in younger patients, and tumors with this amplification were associated with a significantly better OS.
An Integrative Approach to the Current Treatment of HIV-Associated Neurocognitive Disorders and the Implementation of Leukemia Inhibitor Factor as a Mediator of Neurocognitive Preservation
Authors: De Freitas-Suarez Andrés , Espinosa-Ponce Natalia , Alvarez-Roger Natalia , Cabrera-Suarez Arianna Iris , Jiménez-Jordán Guillermo , Vega-Roman Rocio , Inyushin Mikhail , Alves Janaina M.
Published: November 2023

HIV-associated neurocognitive disorders (HANDs) continue to impact patients despite antiretroviral therapy. A combination of antiretroviral therapies can diminish the HIV viral load to near undetectable levels, but fails to preserve neurocognitive integrity. The cytokine leukemia inhibitory factor (LIF) has shown neuroprotective properties that could mitigate neurodegeneration in HANDs. The LIF promotes neurogenesis, neural cell differentiation, and survival. Combination antiretroviral therapy reduces severe forms of HANDs, but neurocognitive impairment persists; additionally, some antiretrovirals have additional adverse neurotoxic effects. The LIF counteracts neurotoxic viral proteins and limits neural cell damage in models of neuroinflammation. Adding the LIF as an adjuvant therapy to enhance neuroprotection merits further research for managing HANDs. The successful implementation of the LIF to current therapies would contribute to achieving a better quality of life for the affected population.
An Alkaline Foregut Protects Herbivores from Latex in Forage, but Increases Their Susceptibility to Bt Endotoxin
Authors: Rajan Vidya
Published: November 2023

About 10% of angiosperms, an estimated 20,000 species, produce latex from ubiquitous isoprene precursors. Latex, an aqueous suspension of rubber particles and other compounds, functions as an antifeedant and herbivory deterrent. It is soluble in neutral to alkaline pH, and coagulates in acidic environments. Here, I propose that foregut-fermenting herbivores such as ruminants, kangaroos, sloths, insect larvae, and tadpoles have adapted to latex in forage with the evolution of alkaline anterior digestive chamber(s). However, they consequently become susceptible to the action of Bacillus thuringiensis (Bt) δ-endotoxin and related bioinsecticides which are activated in alkaline environments. By contrast, hindgut-fermenting herbivores, such as horses and rabbits, have acidic anterior digestive chambers, in which latex coagulates and may cause gut blockage, but in which Bt is not activated. The latex-adapted foregut herbivore vs. latex-maladapted hindgut herbivore hypothesis developed in this paper has implications for hindgut-fermenting livestock and zoo animals which may be provided with latex-containing forage that is detrimental to their gut health. Further, ruminants and herbivorous tadpoles with alkaline anterior chambers are at risk of damage by the supposedly “environmentally friendly” Bt bioinsecticide, which is widely disseminated or engineered into crops which may enter animal feed streams.
Fibrin and Fibrinolytic Enzyme Cascade in Thrombosis: Unravelling the Role
Authors: Singh Rajni , Gautam Prerna , Sharma Chhavi , Osmolovskiy Alexander
Published: November 2023

Blood clot formation in blood vessels (thrombosis) is a major cause of life-threatening cardiovascular diseases. These clots are formed by αA-, βB-, and ϒ-peptide chains of fibrinogen joined together by isopeptide bonds with the help of blood coagulation factor XIIIa. These clot structures are altered by various factors such as thrombin, platelets, transglutaminase, DNA, histones, and red blood cells. Various factors are used to dissolve the blood clot, such as anticoagulant agents, antiplatelets drugs, fibrinolytic enzymes, and surgical operations. Fibrinolytic enzymes are produced by microorganisms (bacteria, fungi, etc.): streptokinase of Streptococcus hemolyticus, nattokinase of Bacillus subtilis YF 38, bafibrinase of Bacillus sp. AS-S20-I, longolytin of Arthrobotrys longa, versiase of Aspergillus versicolor ZLH-1, etc. They act as a thrombolytic agent by either enhancing the production of plasminogen activators (tissue or urokinase types), which convert inactive plasminogen to active plasmin, or acting as plasmin-like proteins themselves, forming fibrin degradation products which cause normal blood flow again in blood vessels. Fibrinolytic enzymes may be classified in two groups, as serine proteases and metalloproteases, based on their catalytic properties, consisting of a catalytic triad responsible for their fibrinolytic activity having different physiochemical properties (such as molecular weight, pH, and temperature). The analysis of fibrinolysis helps to detect hyperfibrinolysis (menorrhagia, renal failure, etc.) and hypofibrinolysis (diabetes, obesity, etc.) with the help of various fibrinolytic assays such as a fibrin plate assay, fibrin microplate assay, the viscoelastic method, etc. These fibrinolytic activities serve as a key aspect in the recognition of numerous cardiovascular diseases and can be easily produced on a large scale with a short generation time by microbes and are less expensive.
The Nature of the Spark Is a Pivotal Element in the Design of a Miller–Urey Experiment
Authors: Ravanbodshirazi Sina , Boutfol Timothée , Safaridehkohneh Neda , Finkler Marc , Mohammadi-Kambs Mina , Ott Albrecht
Published: November 2023

Miller and Urey applied electric sparks to a reducive mixture of CH4, NH3, and water to obtain a complex organic mixture including biomolecules. In this study, we examined the impact of temperature, initial pressure, ammonia concentration, and the spark generator on the chemical profile of a Miller–Urey-type prebiotic broth. We analyzed the broth composition using Gas Chromatography combined with Mass Spectroscopy (GC/MS). The results point towards strong compositional changes with the nature of the spark. Ammonia exhibited catalytic properties even with non-nitrogen-containing compounds. A more elevated temperature led to a higher variety of substances. We conclude that to reproduce such a broth as well as possible, all the studied parameters need to be tightly controlled, the most difficult and important being spark generation.