bluuebunny/crossref_metadata_2025 huggingface dataset:
Design Trade-Offs in Common-Mode Feedback Implementations for Highly Linear Three-Stage Operational Transconductance Amplifiers
Authors: Riad Joseph , Soto-Aguilar Sergio , Estrada-López Johan J. , Moreira-Tamayo Oscar , Sánchez-Sinencio Edgar
Published: April 2021

Fully differential amplifiers require the use of common-mode feedback (CMFB) circuits to properly set the amplifier’s operating point. Due to scaling trends in CMOS technology, modern amplifiers increasingly rely on cascading more than two stages to achieve sufficient gain. With multiple gain stages, different topologies for implementing CMFB are possible, whether using a single CMFB loop or multiple ones. However, the impact on performance of each CMFB approach has seldom been studied in the literature. The aim of this work is to guide the choice of the CMFB implementation topology evaluating performance in terms of stability, linearity, noise and common-mode rejection. We present a detailed theoretical analysis, comparing the relative performance of two CMFB configurations for 3-stage OTA topologies in an implementation-agnostic manner. Our analysis is then corroborated through a case study with full simulation results comparing the two topologies at the transistor level and confirming the theoretical intuition. An active-RC filter is used as an example of a high-linearity OTA application, highlighting a 6 dB improvement in P1dB in the multi-loop implementation with respect to the single-loop case.
Millimeter-Wave Channel Modeling in a Vehicular Ad-Hoc Network Using Bose–Chaudhuri–Hocquenghem (BCH) Code
Authors: Ahmed Arshee , Rasheed Haroon , Liyanage Madhusanka
Published: April 2021

The increase in capacity demand for vehicular communication is generating interest among researchers. The standard spectra allocated to VANET tend to be saturated and are no longer enough for real-time applications. Millimeter-wave is a potential candidate for VANET applications. However, millimeter-wave is susceptible to pathloss and fading, which degrade system performance. Beamforming, multi-input multi-output (MIMO) and diversity techniques are being employed to minimize throughput, reliability and data rate issues. This paper presents a tractable channel model for VANET in which system performance degradation due to error is addressed by concatenated Alamouti space-time block coding (ASTBC) and Bose–Chaudhuri–Hocquenghem (BCH) coding. Two closed-form approximations of bit error rate (BER), one for BCH in Rayleigh fading and the second for BCH with ASTBC, are derived. These expressions comprise SNR and code rate and can be utilized in designing VANET architectures. The results show that the BER using concatenated ASTBC and BCH outmatches the conventional BER ASTBC expression. The analytical results are compared with numerical results, thereby showing the accuracy of our closed-form expressions. The performance of the proposed expressions is evaluated using different code rates.
Miniaturization and Electromagnetic Reliability of Wearable Textile Antennas
Authors: Yao Lan , Li Erhong , Yan Jiangshan , Shan Zhiyong , Ruan Xinyi , Shen Zhiyi , Ren Yueying , Yang Jiahua
Published: April 2021

The wearable textile antenna plays a decisive role in the currently increasingly wireless communication network area. To realize the miniaturization and electromagnetic reliability, a slotted full-textile microstrip antenna was designed and fabricated using the screen printing method. The measured return loss and radiation pattern were tested and compared with the simulated results. Additionally, the adhesion between the silver paste coating as the radiation element and the textile substrate was detected using sticking tape as well as observation by the microscope. To develop the designed antenna in the on-body application, the artificial magnetic conductor (AMC) was designed, optimized and fabricated. The antenna performance results showed that the existence of the AMC had a significant effect in reducing the body coupling and antenna backward radiation.
Procedures and Methodologies for the Control and Improvement of Energy-Environmental Quality in Construction
Authors: Nastasi Benedetto , Mancini Francesco
Published: April 2021

Building performance from an energy and an environmental point of view is fundamental due to the large amount of GHG emissions related to the building sector [...]
Hydrogen Technology on the Polish Electromobility Market. Legal, Economic, and Social Aspects
Authors: Drożdż Wojciech , Elżanowski Filip , Dowejko Jakub , Brożyński Bartosz
Published: April 2021

The aim of this study was to evaluate the motorization market of electric vehicles powered by hydrogen cells in Poland. European conditions of such technology were indicated, as well as original proposals on amendments to the law to increase the development pace of electromobility based on hydrogen cells. There were also presented economic aspects of this economic phenomenon. Moreover, survey research was conducted to examine the preferences of hydrogen and electric vehicle users in 5 primary Polish cities. In this way, the level of social acceptance for the technological revolution based on hydrogen cells and taking place in the motorization sector was determined.
Deep Learning-Based Classification of Fine Hand Movements from Low Frequency EEG
Authors: Bressan Giulia , Cisotto Giulia , Müller-Putz Gernot R. , Wriessnegger Selina Christin
Published: April 2021

The classification of different fine hand movements from electroencephalogram (EEG) signals represents a relevant research challenge, e.g., in BCI applications for motor rehabilitation. Here, we analyzed two different datasets where fine hand movements (touch, grasp, palmar, and lateral grasp) were performed in a self-paced modality. We trained and tested a newly proposed CNN, and we compared its classification performance with two well-established machine learning models, namely, shrinkage-linear discriminant analysis (LDA) and Random Forest (RF). Compared to previous literature, we included neuroscientific evidence, and we trained our Convolutional Neural Network (CNN) model on the so-called movement-related cortical potentials (MRCPs). They are EEG amplitude modulations at low frequencies, i.e., (0.3,3) Hz that have been proved to encode several properties of the movements, e.g., type of grasp, force level, and speed. We showed that CNN achieved good performance in both datasets (accuracy of 0.70±0.11 and 0.64±0.10, for the two datasets, respectively), and they were similar or superior to the baseline models (accuracy of 0.68±0.10 and 0.62±0.07 with sLDA; accuracy of 0.70±0.15 and 0.61±0.07 with RF, with comparable performance in precision and recall). In addition, compared to the baseline, our CNN requires a faster pre-processing procedure, paving the way for its possible use in online BCI applications.
Characterising Momentum Flux Events in High Reynolds Number Turbulent Boundary Layers
Authors: Deshpande Rahul , Marusic Ivan
Published: April 2021

The momentum flux in a canonical turbulent boundary layer is known to have a time-series signature that is characterised by a highly intermittent variation, which includes very short periods of intense flux activity. Here, we study the variation in these flux signal characteristics across almost a decade of flow Reynolds number (Reτ) by analysing datasets acquired using miniature cross-wire probes with matched spatial resolution. The analysis is facilitated by conditionally sampling the signal based on the quadrant (Qi; i = 1–4) and magnitude of the flux, revealing fractional cumulative contribution from Q4 to increase at a much faster rate than from Q2 with Reτ. An episodic description of the flux signal is subsequently undertaken, which associates this rapid increase in Q4 contributions with the emergence of extreme and rare flux events with Reτ. The same dataset is also used to test Townsend’s hypothesis on the active and inactive components of the momentum flux, which are obtained for the first time by implementing a spectral linear stochastic estimation-based decomposition methodology. While the active component is found to be the dominant contributor to the mean momentum flux consistent with Townsend’s hypothesis, the inactive component is found to be small but non-zero, owing to the non-linear interactions associated with the modulation phenomenon. Finally, an episodic description of the active and inactive momentum flux signal is undertaken to highlight the starkly different time series characteristics of the two flux components. The inactive flux signal is found to comprise individual statistically significant events associated with all four quadrants, leading to a small net contribution to the total flux.
Potential Application of Resistant Starch Sorghum in Gluten-Free Pasta: Nutritional, Structural and Sensory Evaluations
Authors: Cervini Mariasole , Gruppi Alice , Bassani Andrea , Spigno Giorgia , Giuberti Gianluca
Published: April 2021

Gluten-free (GF) pasta samples containing rice flour replaced with 0, 5, 10, 15 g/100 g (w/w) of a resistant starch ingredient from annealed sorghum starch (annRS) were formulated. The highest total dietary fiber and RS contents (p < 0.05) were measured in uncooked pasta with 15 g/100 g of annRS addition (15-annRS). After cooking, the 15-annRS pasta was characterized by an RS content of 5.8 g/100 g dry matter, confirming the thermal resistance of annRS. The use of annRS positively influenced the optimal cooking time, the cooking loss, the firmness, and the stickiness of the cooked samples, with not remarkably change in color after cooking. The starch hydrolysis index values decreased as the level of annRS increased. Despite a significant decrease in the overall sensory with increasing levels of annRS, all samples were characterized by a value > 5, which is considered the limit of acceptability. The use of annRS in GF pasta up to 15 g/100 g can contribute to creating GF products with high total dietary fiber content, slowly digestible starch properties, and without drastically compromising the sensory attributes.
Assessing the Potential of Algae Extracts for Extending the Shelf Life of Rainbow Trout (Oncorhynchus mykiss) Fillets
Authors: Sáez María I. , Suárez María D. , Alarcón Francisco J. , Martínez Tomás F.
Published: April 2021

This study evaluates the potential of different algae extracts (Crassiphycus corneus, Cc; Ulva ohnoi, Uo; Arthrospira platensis, Ap; Haematococcus pluvialis, Hp) as additives for the preservation of rainbow trout fillets. The extracts were prepared with different water to ethanol ratios from the four algae species. The highest ferric reducing antioxidant power (FRAP) was observed in Uo extracted in 80% ethanol. Ap aqueous extract also had considerable FRAP activity, in agreement with a high total phenolic content. Radical scavenging activity (DPPH) was higher in Cc 80% ethanol extract, in agreement with a high total carotenoid content. In fact, when the algae aqueous extracts were assayed on the fish fillets, their antioxidant activity exceeded that of ascorbic acid (ASC). All algae extracts delayed microbial growth and lipid oxidation processes in trout fillets throughout the cold storage period compared to controls, and also improved textural parameters, these effects being more evident for Ap and Hp. With respect to the color parameters, the Hp extract prevented the a* values (redness) from decreasing throughout cold storage, a key point when it comes to colored species, not least salmonids. On the other hand, the Ap extract was not as effective as the rest of treatments in avoiding a* and b* decrease throughout the storage period, and thereby the color parameters were impaired. The results obtained, together with the natural origin and the viability for large-scale cultivation, make algae extracts interesting fish preservative agents for the food industry.
Optimising Repeated Exposure: Determining Optimal Exposure Frequency for Introducing a Novel Vegetable among Children
Authors: Karagiannaki Klelia , Ritz Christian , Jensen Louise Grønhøj Hørbye , Tørsleff Ellen Hyldgaard , Møller Per , Hausner Helene , Olsen Annemarie
Published: April 2021

Fruit and vegetables are important components of a healthy diet, but unfortunately many children are not consuming enough to meet the recommendations. Therefore, it is crucial to develop strategies towards increasing the acceptance of this food group. This study aims to investigate the effect of different repeated exposure frequencies on fruit and vegetable acceptance using a novel vegetable, daikon, among 3–6-year-old children. One hundred and fifty-nine children participated in this study. Eight kindergarten teams were assigned to one of the following groups: Three different intervention groups with varying exposure frequencies, but all receiving seven exposures: Twice a week (n = 47), once a week (n = 32) and once every second week (n = 30), and a control group (n = 50). Liking and familiarity of daikon and other vegetables (cucumber, celery, celeriac, broccoli, cauliflower and beetroot) were assessed at baseline, post-intervention and two follow up sessions (3 and 6 months) to test for potential generalisation effects and observe the longevity of the obtained effects. Intake of daikon was measured at all exposures and test sessions. Results showed significant increases (p ≤ 0.05) in liking and intake of daikon for all three frequencies and the control group. Over the exposures, intake of daikon increased until the 4th exposure for all the groups, where a plateau was reached. No systematic generalisation effects were found. Repeated exposure was a successful approach to increase liking and intake of a novel vegetable with all exposure frequencies to be effective, and no particular exposure frequency can be recommended. Even the few exposures the control group received were found to be sufficient to improve intake and liking over 6 months (p ≤ 0.05), indicating that exposures to low quantities of an unfamiliar vegetable may be sufficient.
Structural and Technological Approach to Reveal the Role of the Lipid Phase in the Formation of Soy Emulsion Gels with Chia Oil
Authors: Herrero Ana M. , Ruiz-Capillas Claudia
Published: April 2021

Considerable attention has been paid to emulsion gels (EGs) in recent years due to their interesting applications in food. The aim of this work is to shed light on the role played by chia oil in the technological and structural properties of EGs made from soy protein isolates (SPI) and alginate. Two systems were studied: oil-free SPI gels (SPI/G) and the corresponding SPI EGs (SPI/EG) that contain chia oil. The proximate composition, technological properties (syneresis, pH, color and texture) and structural properties using Raman spectroscopy were determined for SPI/G and SPI/EG. No noticeable (p > 0.05) syneresis was observed in either sample. The pH values were similar (p > 0.05) for SPI/G and SPI/EG, but their texture and color differed significantly depending on the presence of chia oil. SPI/EG featured significantly lower redness and more lightness and yellowness and exhibited greater puncture and gel strengths than SPI/G. Raman spectroscopy revealed significant changes in the protein secondary structure, i.e., higher (p < 0.05) α-helix and lower (p < 0.05) β-sheet, turn and unordered structures, after the incorporation of chia oil to form the corresponding SPI/EG. Apparently, there is a correlation between these structural changes and the textural modifications observed.
No Time to Age: Uncoupling Aging from Chronological Time
Authors: Larocca Dana , Lee Jieun , West Michael D. , Labat Ivan , Sternberg Hal
Published: April 2021

Multicellular life evolved from simple unicellular organisms that could replicate indefinitely, being essentially ageless. At this point, life split into two fundamentally different cell types: the immortal germline representing an unbroken lineage of cell division with no intrinsic endpoint and the mortal soma, which ages and dies. In this review, we describe the germline as clock-free and the soma as clock-bound and discuss aging with respect to three DNA-based cellular clocks (telomeric, DNA methylation, and transposable element). The ticking of these clocks corresponds to the stepwise progressive limitation of growth and regeneration of somatic cells that we term somatic restriction. Somatic restriction acts in opposition to strategies that ensure continued germline replication and regeneration. We thus consider the plasticity of aging as a process not fixed to the pace of chronological time but one that can speed up or slow down depending on the rate of intrinsic cellular clocks. We further describe how germline factor reprogramming might be used to slow the rate of aging and potentially reverse it by causing the clocks to tick backward. Therefore, reprogramming may eventually lead to therapeutic strategies to treat degenerative diseases by altering aging itself, the one condition common to us all.
A New Pathogenic Variant in POU3F4 Causing Deafness Due to an Incomplete Partition of the Cochlea Paved the Way for Innovative Surgery
Authors: Tekin Ahmet M. , Matulic Marco , Wuyts Wim , Assadi Masoud Zoka , Mertens Griet , Rompaey Vincent van , Li Yongxin , Heyning Paul van de , Topsakal Vedat
Published: April 2021

Incomplete partition type III (IP-III) is a relatively rare inner ear malformation that has been associated with a POU3F4 gene mutation. The IP-III anomaly is mainly characterized by incomplete separation of the modiolus of the cochlea from the internal auditory canal. We describe a 71-year-old woman with profound sensorineural hearing loss diagnosed with an IP-III of the cochlea that underwent cochlear implantation. Via targeted sequencing with a non-syndromic gene panel, we identified a heterozygous c.934G > C p. (Ala31Pro) pathogenic variant in the POU3F4 gene that has not been reported previously. IP-III of the cochlea is challenging for cochlear implant surgery for two main reasons: liquor cerebrospinalis gusher and electrode misplacement. Surgically, it may be better to opt for a shorter array because it is less likely for misplacement with the electrode in a false route. Secondly, the surgeon has to consider the insertion angles of cochlear access very strictly to avoid misplacement along the inner ear canal. Genetic results in well describes genotype-phenotype correlations are a strong clinical tool and as in this case guided surgical planning and robotic execution.
Novel lincRNA Discovery and Tissue-Specific Gene Expression across 30 Normal Human Tissues
Authors: Chen Xianfeng , Sun Zhifu
Published: April 2021

Long non-coding RNAs (lncRNAs) are a large class of gene transcripts that do not code proteins; however, their functions are largely unknown and many new lncRNAs are yet to be discovered. Taking advantage of our previously developed, super-fast, novel lncRNA discovery pipeline, UClncR, and rich resources of GTEx RNA-seq data, we performed systematic novel lincRNA discovery for over 8000 samples across 30 tissue types. We conducted novel detection for each major tissue type first and then consolidated the novel discoveries from all tissue types. These novel lincRNs were profiled and analyzed along with known genes to identify tissue-specific genes in 30 major human tissue types. Thirteen sub-brain regions were also analyzed in a similar manner. Our analysis revealed thousands to tens of thousands of novel lincRNAs for each tissue type. These lincRNAs could define each tissue type’s identity and demonstrated their reliability and tissue-specific expression. Tissue-specific genes were identified for each major tissue type and sub-brain region. The tissue-specific genes clearly defined each respective tissue’s unique function and could be used to expand the interpretation of non-coding SNPs from genome-wide association (GWAS) studies.
Identification and Characterization of Alternatively Spliced Transcript Isoforms of IRX4 in Prostate Cancer
Authors: Fernando Achala , Liyanage Chamikara , Moradi Afshin , Janaththani Panchadsaram , Batra Jyotsna
Published: April 2021

Alternative splicing (AS) is tightly regulated to maintain genomic stability in humans. However, tumor growth, metastasis and therapy resistance benefit from aberrant RNA splicing. Iroquois-class homeodomain protein 4 (IRX4) is a TALE homeobox transcription factor which has been implicated in prostate cancer (PCa) as a tumor suppressor through genome-wide association studies (GWAS) and functional follow-up studies. In the current study, we characterized 12 IRX4 transcripts in PCa cell lines, including seven novel transcripts by RT-PCR and sequencing. They demonstrate unique expression profiles between androgen-responsive and nonresponsive cell lines. These transcripts were significantly overexpressed in PCa cell lines and the cancer genome atlas program (TCGA) PCa clinical specimens, suggesting their probable involvement in PCa progression. Moreover, a PCa risk-associated SNP rs12653946 genotype GG was corelated with lower IRX4 transcript levels. Using mass spectrometry analysis, we identified two IRX4 protein isoforms (54.4 kDa, 57 kDa) comprising all the functional domains and two novel isoforms (40 kDa, 8.7 kDa) lacking functional domains. These IRX4 isoforms might induce distinct functional programming that could contribute to PCa hallmarks, thus providing novel insights into diagnostic, prognostic and therapeutic significance in PCa management.
Refugees’ Opinions about Healthcare Services: A Case of Turkey
Authors: Tengilimoğlu Dilaver , Zekioğlu Aysu , Budak Fatih , Eriş Hüseyin , Younis Mustafa
Published: April 2021

Background: Migration is one of the most important social events in human history. In recent years, Turkey hosted a high number of asylum seekers and refugees, primarily because of continuing wars and radical social changes in the Middle East. Methods: Using a random sampling method, Syrian refugees aged 18 and over, who can communicate in Turkish, were reached via personal contact and a total of 714 refugees participated in the study voluntarily. Results: Turkey has mounted with some success and to point out that even though participating refugees in both provinces are young and healthy, almost 50% have bad or worse health status, 61% have chronic diseases, and 55% need regular medication. Participating refugees living in Şanlıurfa stated that ‘Hospitals are very clean and tidy.’ (3.80 ± 0.80). The answers given to the following statements had the highest mean for the participating refugees living in Kilis; ‘Hospitals are clean and tidy.’ (3.22 ± 1.25). Conclusion: Due to financial and human resource deficiencies, there are problems in providing preventive and therapeutic health services, especially to refugees living outside the refugee camps in bad conditions. It is important that refugees are encouraged to apply to family health and community health centers in this context.
The Implementation of the WHO Mental Health Gap Intervention Guide (mhGAP-IG) in Ukraine, Armenia, Georgia and Kyrgyz Republic
Authors: Pinchuk Irina , Yachnik Yulia , Kopchak Oksana , Avetisyan Kristine , Gasparyan Khachatur , Ghazaryan Gayane , Chkonia Eka , Panteleeva Lilya , Guerrero Anthony , Skokauskas Norbert
Published: April 2021

Despite the increasing burden of mental disorders, a lot of people worldwide suffer a gap in receiving necessary care in these countries. To close this gap, the WHO has developed mhGAP training modules aimed at scaling up mental health and substance use disorders services, especially in low- and middle-income countries. This article presents the experience of implementing the Mental Health Gap Action Programme (mhGAP) in Ukraine, Armenia, Georgia, and Kyrgyz Republic. Data were gathered from an electronic questionnaire administered to representatives from higher educational institutions where the Mental Health Gap Action Programme Intervention Guide (mhGAP-IG) was implemented in existing curricula for medical students, interns, and residents in family medicine and neurology, practicing physicians, and master’s program in mental health students. More than 700 students went through the programs that provided the feedback. Evaluations of program effectiveness mainly involved standard discipline tests or pre- and post-tests proposed in the mhGAP trainer manual. This finding suggested that mhGAP-IG can be successfully adapted and implemented both on undergraduate and on postgraduate education levels and among medical and nonmedical specialists. Future evaluations need to more definitively assess the clinical effectiveness of mhGAP-IG implementation.
Long-Term Sustainability of Water Cellars in Traditional Chinese Villages: Factors Influencing Continuous Use and Effective Water Management Initiatives
Authors: Zhou Weinan , Matsumoto Kunihiko , Sawaki Masanori
Published: April 2021

Traditional rainwater harvesting systems have seen a shift of emphasis in recent years. While recognizing its social, economic and environmental contributions, sustainable use in a modern context can be vulnerable. Through a case study, this study focuses on the long-term sustainability of water cellars in traditional villages if reliable piped water is introduced. The aim is to discern the factors and renovation methods that influence residents’ willingness to continue using these water cellars. The results show that the overall willingness to use them is very low. However, regardless of their continued use or non-use, only a few residents would landfill them. Most residents were interested in their renovation, especially regarding simplifying rainwater harvesting methods. In addition, the management time for rainwater harvesting and heritage identity is positively correlated with the willingness for sustainable use; conversely, the identification of the environmental contribution has no positive correlation. Given these findings, we propose carrying out effective renovation that changes the rainwater catchment surface to roofs and increases residents’ awareness that water cellars can only be heritage if they are in use. By defining the long-term sustainability of a water cellar, this study shows how a quantitative approach focusing on heritage users can offer important insights into a constructive evolution rather than a destructive reconstruction under the influence of modernization. Finally, this study provides planners and water resource managers with effective, sustainable management practices for water cellars as well as similar systems in a historical context.
Effect of Neighborhood and Individual-Level Socioeconomic Factors on Colorectal Cancer Screening Adherence
Authors: Mayhand Kiara N. , Handorf Elizabeth A. , Ortiz Angel G. , Gonzalez Evelyn T. , Devlin Amie , Sorice Kristen A. , Esnaola Nestor , Fisher Susan , Lynch Shannon M.
Published: April 2021

Despite the effectiveness of screenings in reducing colorectal cancer (CRC) mortality, ~25% of US adults do not adhere to screening guidelines. Prior studies associate socioeconomic status (SES) with low screening adherence and suggest that neighborhood deprivation can influence CRC outcomes. We comprehensively investigated the effect of neighborhood SES circumstances (nSES), individual SES, and race/ethnicity on adherence to CRC screening in a multiethnic cross-sectional study. Participant surveys assessing 32 individual-level socioeconomic and healthcare access measures were administered from 2017 to 2018. Participant data were joined with nine nSES measures from the US Census at the census tract level. Univariate, LASSO, and multivariable mixed-effect logistic regression models were used for variable reduction and evaluation of associations. The total study population included 526 participants aged 50–85; 29% of participants were non-adherent. In the final multivariable model, age (p = 0.02) and Non-Hispanic Black race (p = 0.02) were associated with higher odds of adherence. Factors associated with lower adherence were home rental (vs. ownership) (p = 0.003), perception of low healthcare quality (p = 0.006), no routine checkup within two years (p = 0.002), perceived discrimination (p = 0.02), and nSES deprivation (p = 0.02). After comprehensive variable methods were applied, socioeconomic indicators at the neighborhood and individual level were found to contribute to low CRC screening adherence.
Rapid Detection of Clostridium botulinum in Food Using Loop-Mediated Isothermal Amplification (LAMP)
Authors: Chen Yufei , Li Hao , Yang Liu , Wang Lei , Sun Ruyi , Shearer Julia E. S. , Sun Fengjie
Published: April 2021

Botulinum neurotoxins are considered as one of the most potent toxins and are produced by Clostridium botulinum. It is crucial to have a rapid and sensitive method to detect the bacterium Clostridium botulinum in food. In this study, a rapid detection assay of C. botulinum in food using loop-mediated isothermal amplification (LAMP) technology was developed. The optimal primers were identified among three sets of primers designed specifically based on the partial ntnh gene encoding nontoxic-nonhaemagglutinin (NTNH) for rapid detection of the target DNA in plasmids. The optimal temperature and reaction time of the LAMP assay were determined to be 64 °C and 60 min, respectively. The chemical kit could be assembled based on these optimized reaction conditions for quick, initial high-throughput screening of C. botulinum in food samples. The established LAMP assay showed high specificity and sensitivity in detecting the target DNA with a limit of 0.0001 pg/ul (i.e., ten times more sensitive than that of the PCR method) and an accuracy rate of 100%. This study demonstrated a potentially rapid, cost-effective, and easy-operating method to detect C. botulinum in food and clinical samples based on LAMP technology.
Landscape Pattern Evolution Processes of Wetlands and Their Driving Factors in the Xiong’an New Area of China
Authors: Yang Miao , Gong Jiaguo , Zhao Yong , Wang Hao , Zhao Cuiping , Yang Qin , Yin Yingshen , Wang Ying , Tian Bo
Published: April 2021

Wetland landscape patterns are the result of various ecological and hydrological processes. Based on the land use landscape types from 1980 to 2017, a transfer matrix, landscape pattern analysis index, and principal component analysis were used to analyze the landscape pattern evolution in the Xiong’an New Area of China, which has a large area with a lake and river wetlands. The results showed that the wetland area has changed greatly since 2000 and the beach land has decreased greatly, while the area of the lake and river wetlands has increased slightly. Beach land was the dominant landscape type of the wetland. The dominant degree of the wetland landscape showed a slightly decreasing trend, and the patches tended to be scattered. The shape complexity of the ponds was the lowest, while that of rivers was the highest. The fragmentation degree of the wetland patches increased, the proportion of landscape types tended to be equalized, and the landscape heterogeneity increased. The leading factors of the wetland landscape change can be summarized as socioeconomic, meteorological, and hydrological processes, with a cumulative contribution rate of 85.3%, among which socioeconomic development was the most important factor. The results have important guiding significance for the ecological restoration and management of wetlands in the Xiong’an New Area and other wetland ecosystems with rivers and lakes.
A Systematic Review and Meta-Analysis of Associated Factors of Gender-Based Violence against Women in Sub-Saharan Africa
Authors: Muluneh Muluken Dessalegn , Francis Lyn , Agho Kingsley , Stulz Virginia
Published: April 2021

A systematic review and meta-analysis were employed to address the associated factors of gender-based violence (GBV) in sub-Saharan African (SSA) countries. The Preferred Reporting Items for Systematic reviews and Meta-Analysis guidelines were followed. Ovid Medline, CINAHL, Cochrane Central, Embase, Scopus and Web of Science were used to source articles with stringent eligibility criteria. A total of 4931 studies were found and 50 studies met the inclusion criteria. Pooled meta-analyses revealed that low educational attainment, higher alcohol consumption, substance use, history of child and family abuse, limited decision-making skills, experiencing depression, males having multiple sexual partners, and younger age were found to be individual- and family-associated factors that increase the experiences of GBV. Community tolerant attitudes to violence, women’s unemployment, being Muslim, lower socioeconomic class, food and social insecurity were found to be community- and societal-associated factors of GBV. Alcohol consumption, low educational attainment, experiencing depression, being younger, a history of child and family abuse, tolerant attitudes to violence, and low socioeconomic status were poignant factors associated with GBV amongst women in SSA countries. The need to develop a multipronged approach of intervention is a top priority in SSA to reach the Sustainable Development Goals’ (SDGs) target of 2030 to eliminate all forms of violence. Socio-behavioural change communication interventions at individual and community levels need to be introduced, and interventions need to address the prevention of child and family abuse and increase women’s feelings of empowerment in order to prevent GBV in SSA.
Twenty-Four-Year Trends in Family and Regional Disparities in Fruit, Vegetable and Sugar-Sweetened Beverage Consumption among Adolescents in Belgium
Authors: Rouche Manon , Dierckens Maxim , Desbouys Lucille , Pedroni Camille , Lebacq Thérésa , Godin Isabelle , Deforche Benedicte , Castetbon Katia
Published: April 2021

Dietary habits are influenced by various determinants that may evolve over time. This study aimed to examine, among adolescents in Belgium, trends in the dietary habits between 1990 and 2014 and to determine changes in family and regional disparities related to diet during this time period. In the 1990, 2002 and 2014 cross-sectional “Health Behaviour in School-aged Children” (HBSC) surveys, food consumption was estimated using a short Food Frequency Questionnaire. The Relative Index of Inequality (RII) enabled quantification of the gradients of inequality related to the family structure and to the region for non-daily fruit and vegetable and daily sugar-sweetened beverage (SSB) consumption. Between 1990 and 2014, the prevalence of non-daily fruit consumption increased from 27.7% to 60.6%, whereas the daily SSB consumption decreased from 58.9% to 34.8%. Over time, a downward trend in family disparities (p = 0.007) was observed for daily fruit consumption (RII: 1.58 (1.33–1.88) to 1.18 (1.13–1.23)). An upward trend in region-related disparities (p < 0.001) for SSB was found (RII: 1.15 (1.07–1.23) to 1.37 (1.28–1.47)). The overall trend of increasing disparities when dietary habits improved and decreasing disparities when dietary habits worsened highlights the need to implement actions that improve overall dietary habits while ensuring that disparities do not increase.
Use of Digital Media for Home-Based Sports Activities during the COVID-19 Pandemic: Results from the German SPOVID Survey
Authors: Mutz Michael , Müller Johannes , Reimers Anne K.
Published: April 2021

Health authorities recommend digital tools for home-based sport and exercise routines to stay active and healthy during the COVID-19 pandemic. The study investigates the prevalence, duration, most popular activities, and social selectivity of home-based digital sport and its contribution to overall levels of sporting activity during the pandemic. It is based on cross-sectional survey data (n = 1508), representing the population >14 years living in Germany. Data collection took place in October 2020, using computer-assisted web interviewing. Results show that overall, 23% of respondents used digital media for sports activities at least one time during the COVID-19 pandemic. Numbers increased during the lockdown and decreased afterwards. People engaged in a variety of fitness workouts, most frequently practiced with the help of publicly accessible fitness videos from video-sharing platforms. Digital sports practitioners are younger, better educated, and financially better off. Females are overrepresented. Individuals engaged in digital sports achieved 30 min/week more sports activity during the pandemic compared to individuals solely involved in offline sports. Hence, home-based digital sports activities were a popular means to stay active, particularly in the period of the lockdown. Strong social disparities indicate that the possible health benefits of digital sports only reach out to particular population groups.
Can the Timed and Targeted Counseling Model Improve the Quality of Maternal and Newborn Health Care? A Process Analysis in the Rural Hoima District in Uganda
Authors: Babughirana Geoffrey , Gerards Sanne , Mokori Alex , Baigereza Isaac Charles , Mukembo Alex , Rukanda Grace , Kremers Stef P. J. , Gubbels Jessica
Published: April 2021

Each year, more than half a million women die worldwide from causes related to pregnancy and childbirth, and nearly 4 million newborns die within 28 days of birth. In Uganda, 15 women die every single day from pregnancy and childbirth-related causes, 94 babies are stillborn, and 81 newborn babies die. Cost-effective solutions for the continuum of care can be achieved through Village Health Teams to improve home care practices and health care-seeking behavior. This study aims at examining the implementation of the timed and targeted counseling (ttC) model, as well as improving maternal and newborn health care practices. We conducted a quantitative longitudinal study on pregnant mothers who were recruited on suspicion of the pregnancy and followed-up until six weeks post-delivery. The household register was the primary data source, which was collected through a secondary review of the ttC registers. All outcome and process variables were analyzed using descriptive statistics. The study enrolled 616 households from 64 villages across seven sub-counties in Hoima district with a 98.5% successful follow-up rate. Over the course of the implementation period of ttC, there was an increase of 29.6% in timely 1st antenatal care, 28.7% in essential newborn care, 25.5% in exclusive breastfeeding, and 17.5% in quality of antenatal care. All these improvements were statistically significant. The findings from this study show that the application of the ttC model through Village Health Teams has great potential to improve the quality of antenatal and newborn care and the health-seeking practices of pregnant and breastfeeding mothers in rural communities.
We Want More Than Life-Sustaining Treatment during End-of-Life Care: Focus-Group Interviews
Authors: Kim Mirinae , Kim Minju
Published: April 2021

We qualitatively investigated end-of-life care needs. Data were collected via focus-group interviews with three groups: young adults, middle-aged adults, and older adults. The key question was, “What kind of care would you like to receive at the end of life?” Interview data were transcribed and analyzed using content analysis. End-of-life care needs were classified into six categories: life-sustaining treatment needs, physical care needs, emotional care needs, environmental needs, needs for respect, and needs for preparation for death. Because the Korean culture is family-oriented and talking about death is taboo, Korean patients at the end of their life do not make decisions about life-sustaining treatment or actively prepare for death. Therefore, to provide proper end-of-life care, conversations and shared decision-making among patients and their families are crucial. Further, we must respect patients’ dignity and help them achieve a good death by understanding patients’ basic care preferences. Future research should continue examining end-of-life care needs that reflect the social and cultural context of Korea to inform instrument development.
Hand Hygiene during the Early Neonatal Period: A Mixed-Methods Observational Study in Healthcare Facilities and Households in Rural Cambodia
Authors: Nalule Yolisa , Buxton Helen , Macintyre Alison , Ir Por , Pors Ponnary , Samol Channa , Leang Supheap , Dreibelbis Robert
Published: April 2021

Background: Globally, infections are the third leading cause of neonatal mortality. Predominant risk factors for facility-born newborns are poor hygiene practices that span both facilities and home environments. Current improvement interventions focus on only one environment and target limited caregivers, primarily birth attendants and mothers. To inform the design of a hand hygiene behavioural change intervention in rural Cambodia, a formative mixed-methods observational study was conducted to investigate the context-specific behaviours and determinants of handwashing among healthcare workers, and maternal and non-maternal caregivers along the early newborn care continuum. Methods: Direct observations of hygiene practices of all individuals providing care to 46 newborns across eight facilities and the associated communities were completed and hand hygiene compliance was assessed. Semi-structured interactive interviews were subsequently conducted with 35 midwives and household members to explore the corresponding cognitive, emotional and environmental factors influencing the observed key hand hygiene behaviours. Results: Hand hygiene opportunities during newborn care were frequent in both settings (n = 1319) and predominantly performed by mothers, fathers and non-parental caregivers. Compliance with hand hygiene protocol across all caregivers, including midwives, was inadequate (0%). Practices were influenced by the lack of accessible physical infrastructure, time, increased workload, low infection risk perception, nurture-related motives, norms and inadequate knowledge. Conclusions: Our findings indicate that an effective intervention in this context should be multi-modal to address the different key behaviour determinants and target a wide range of caregivers.
Retrieval and Evaluation of Chlorophyll-a Concentration in Reservoirs with Main Water Supply Function in Beijing, China, Based on Landsat Satellite Images
Authors: Lai Yuequn , Zhang Jing , Song Yongyu , Gong Zhaoning
Published: April 2021

Remote sensing retrieval is an important technology for studying water eutrophication. In this study, Guanting Reservoir with the main water supply function of Beijing was selected as the research object. Based on the measured data in 2016, 2017, and 2019, and Landsat-8 remote sensing images, the concentration and distribution of chlorophyll-a in the Guanting Reservoir were inversed. We analyzed the changes in chlorophyll-a concentration of the reservoir in Beijing and the reasons and effects. Although the concentration of chlorophyll-a in the Guanting Reservoir decreased gradually, it may still increase. The amount and stability of water storage, chlorophyll-a concentration of the supply water, and nitrogen and phosphorus concentration change are important factors affecting the chlorophyll-a concentration of the reservoir. We also found a strong correlation between the pixel values of adjacent reservoirs in the same image, so the chlorophyll-a estimation model can be applied to each other.
Effect of Myofascial Therapy on Pain and Functionality of the Upper Extremities in Breast Cancer Survivors: A Systematic Review and Meta-Analysis
Authors: Lara-Palomo Inmaculada Carmen , Castro-Sánchez Adelaida María , Córdoba-Peláez Marta María , Albornoz-Cabello Manuel , Ortiz-Comino Lucía
Published: April 2021

(1) Objective: The purpose was to analyze the effectiveness of myofascial therapy on musculoskeletal pain and functionality of the upper extremities in female breast cancer survivors, and to evaluate the changes in range of motion, quality of life, and mood state of these patients. (2) Methods: Systematic searches were performed on the MEDLINE/PubMed, Web of Science, Scopus, and Physiotherapy Evidence Databases for articles published until October 2020, in order to identify randomized controlled trials which analyzed the effectiveness of myofascial therapy as compared to a control group, passive treatment, placebo, or another intervention, and allowed co-interventions on female breast cancer survivors. Two reviewers examined the sources individually, calculated the risk of bias and extracted the data (PROSPERO number CRD42020215823). (3) Results: A total of eight RCTs were included. The results suggested that myofascial therapy does not have a greater statistically significant immediate effect on pain intensity (SMD: −0.15; 95% CI −0.48, 0.19), functionality (SMD: −0.17; 95% CI −0.43, 0.09) and range of motion in flexion (SMD: 0.30; 95% CI −0.13, 0.74) than an inactive, passive treatment or another intervention. However, a statistically significant result was observed for the abduction shoulder in favor of the experimental group (SMD: 0.46; 95% CI 0.05, 0.87; p = 0.03). (4) Conclusion: In general, although we found greater overall effects in support of the intervention with myofascial therapy than other control groups/types of interventions, the subgroup analysis revealed inconsistent results supporting myofascial therapy applied to breast cancer survivors.
Association of Body Composition with Type 2 Diabetes: A Retrospective Chart Review Study
Authors: Lin Chia-Ling , Yu Neng-Chun , Wu Hsueh-Ching , Lee Yung-Yen , Lin Wan-Chun , Chiu I-Ying , Chien Wu-Chien , Liu Yuan-Ching
Published: April 2021

This study analyzed the body composition of individuals with type 2 diabetes (T2DM). In this retrospective chart review study, body composition was measured through multifrequency bioelectrical impedance analysis (InBody 770). Body composition assessments were conducted in individuals with T2DM, who were aged ≥18 years. The parameters included body mass index (BMI), body fat mass (BFM), fat-free mass (FFM), visceral fat area, percent body fat (PBF), appendicular skeletal muscle mass (ASM), and skeletal muscle index (SMI). One-way ANOVA and independent t-tests were used to calculate differences in body composition distribution by age and sex. A total of 2404 participants were recruited. The prevalence of overall low muscle mass and sarcopenic obesity was 28.0% and 18.7%, respectively, which increased with age. The overall prevalence of obesity when PBF was used was 71.5%, which was higher than that when BMI was applied (32.4%). The normal BMI group exhibited a prevalence of low muscle mass of 55.6% and sarcopenic obesity of 34.8%. For both men and women, bodyweight, BFM, FFM, ASM, and SMI all decreased with age. The prevalence of low muscle mass and sarcopenic obesity was high in older adults and people with normal BMI. Using BMI to assess obesity and determine insufficient muscle mass underestimates the prevalence of obesity and neglects the problems of sarcopenia and high body fat in people with normal BMI.