bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the Local Damage Characteristics of Steel Box Girder Structures Under the Effects of Explosive Shock Waves
Authors: Qu Shouyi , Song Yumin
Published: January 2025

This study investigates the local damage characteristics and influencing factors of steel box girder structures under explosive shock waves. The single-box, double-chamber steel box girder commonly used in urban road bridges was chosen as the research object. Based on model validation of the explosion test values of a 1:10 scaled-down model of the steel box girder, a 1:1 numerical model of the steel box girder structure was established. The research analyzed failure modes under varying explosive charge weights and detonation locations. The results showed that failure primarily occurred in the top plate, base plate, and internal partitions, with the top plate experiencing the most severe damage due to direct impact. The effectiveness of transverse and longitudinal partitions in mitigating damage was highlighted, with unpartitioned sections exhibiting up to a 70% increase in damage area. Additionally, stiffening ribs influenced the deflection of base plate cracks, with maximum offset distances ranging from 0.5 m to 1.5 m as explosive weight increased. These findings emphasize the critical role of structural features in enhancing the blast resistance of steel box girder bridges, providing valuable insights for improving protective designs against explosive threats.
Risk Perception and Barriers to Electric Scooter Prevalence
Authors: Shahin Fadi , Elias Wafa
Published: January 2025

Micro-mobility, which includes small, lightweight vehicles such as bicycles, electric scooters, and electric bikes, has emerged as a key component of modern urban transportation over the last decade. ESs have transformed how people navigate cities by offering an eco-friendly alternative to traditional transport, improving last-mile connectivity, and reducing traffic congestion. However, they also present challenges related to safety, infrastructure, and regulation. The rising crash rates involving electric scooters pose a significant public safety concern, driven by their novelty and limited research on associated risks. This study investigates factors influencing the adoption and use of electric scooter-sharing services, emphasizing risk perception, cultural norms, technological familiarity, and physical infrastructure. It also examines travel behaviors, common risks, and barriers to adoption. Using data from 254 Israeli participants, including 50 electric scooter users, the research highlights that 48% of users experienced near-miss incidents, and 38% used scooters on vehicular roads. The primary risk was identified as dangerous driver behavior on these roads, while the key barrier to adoption was a high perception of risk or low sense of safety. A structural equation model revealed that risk perception is influenced by gender-related attitudes and subjective norms, which indirectly diminish positive attitudes toward electric scooters and willingness to share and use them. The findings emphasize the importance of a safe physical infrastructure in fostering positive attitudes and promoting electric scooter use. This research provides valuable insights into mitigating risks and improving the adoption of electric scooters as a sustainable micro-mobility option.
Multi-Dimensional Double Deep Dynamic Q-Network with Aligned Q-Fusion for Dual-Ring Barrier Traffic Signal Control
Authors: Zheng Qiming , Xu Hongfeng , Chen Jingyun , Zhang Kun
Published: January 2025

Model-free deep reinforcement learning (MFDRL) is well-suited for real-time traffic signal control (RTSC), as it is a sequential decision problem where the environment is difficult to be a priori modeled, but has performance metrics sufficing as rewards. Previous studies have not ideally employed MFDRL systems at typical intersections with a dual-ring barrier phase structure (DRBPS) and second-by-second signal operation. DRBPS allows phases to time flexibly while satisfying signal timing constraints in engineering, making it complicated yet common in real-world applications. This study proposes an MFDRL method, termed MD4-AQF, to address the RTSC problem under DRBPS. The state can be represented as a 4 × 30 × 4 × 4 array. We define action based on “decision point aligning” to produce a consistent action space that controls dual-ring concurrent phases simultaneously. We developed a training algorithm based on a “multi-dimensional Q-network” that reduces the number of learnable actions from 600+ to 52. We designed action selection based on “aligned Q-fusion” to end two lagging phases simultaneously with a shared compromise sub-action. In simulation experiments, MD4-AQF trains an agent to improve average vehicle delay from 135 s to 48 s. It surpasses another MFDRL ablated method by 14%, and a fully actuated conventional method by 19%.
The Effects of Cushioning Properties on Parameters of Gait in Habituated Females While Walking and Running
Authors: Macdermid Paul William , Walker Stephanie Julie , Cochrane Darryl
Published: January 2025

The purpose of this study was to compare the mechanical properties of a non-cushioned minimalistic shoe and cushioned shoe during walking at 6 and running at 10 and 14 km∙h−1 in habituated female runners. Twelve habituated female runners completed two trials (cushioned shoe vs. minimalist shoe) with three within-trial speeds (6, 10, and 14 km∙h−1) in a counter-balanced design. Flexible pressure insole sensors were used to determine kinetic variables (peak vertical impact force, average loading rate, active vertical peak force, time to active peak vertical force, and impulse) and spatiotemporal variables (stride duration, cadence, ground contact time, swing time, and time to midstance). Cushioned running shoes exhibited greater energy absorption (690%), recovered energy (920%), and heat dissipation (350%). The cushioned shoes significantly reduced peak vertical impact (~12%) and average loading rate (~11%) at running speeds 10–14 km∙h−1. However, these effects were not observed during walking, nor did the cushioned shoes influence peak active force, impulse, stride duration, ground contact or swing time. Cushioned running shoes provide significant benefits in energy absorption, energy recovery, and heat dissipation, which decrease impact-related forces and loading rates in female runners without changing the spatiotemporal variables of gait.
RSVQ-Diffusion Model for Text-to-Remote-Sensing Image Generation
Authors: Gao Xin , Fu Yao , Jiang Xiaonan , Wu Fanlu , Zhang Yu , Fu Tianjiao , Li Chao , Pei Junyan
Published: January 2025

Despite significant challenges, the text-guided remote sensing image generation method shows great potential in many practical applications such as generative adversarial networks in remote sensing tasks; generated images still face challenges such as low realism, face challenges, and unclear details. Moreover, the inherent spatial complexity of remote sensing images and the limited scale of publicly available datasets make it particularly challenging to generate high-quality remote sensing images from text descriptions. To address these challenges, this paper proposes the RSVQ-Diffusion model for remote sensing image generation, achieving high-quality text-to-remote-sensing image generation applicable for target detection, simulation, and other fields. Specifically, this paper designs a spatial position encoding mechanism to integrate the spatial information of remote sensing images during model training. Additionally, the Transformer module is improved by incorporating a short-sequence local perception mechanism into the diffusion image decoder, addressing issues of unclear details and regional distortions in generated remote sensing images. Compared with the VQ-Diffusion model, our proposed model achieves significant improvements in the Fréchet Inception Distance (FID), the Inception Score (IS), and the text–image alignment (Contrastive Language-Image Pre-training, CLIP) scores. The FID score successfully decreased from 96.68 to 90.36; the CLIP score increased from 26.92 to 27.22, and the IS increased from 7.11 to 7.24.
Effect of Pressure Regulating Mechanism on Screw Pressing Efficiency and Production Capacity in Sea Buckthorn (Hippophae rhamnoides) Juice Extraction
Authors: Kassenov Amirzhan , Muratzhankyzy Nazerke , Mursalykova Maigul , Kokayeva Gulnara , Tokaev Serik
Published: January 2025

This study addresses the optimization of juice extraction from sea buckthorn (Hippophae rhamnoides) to meet the growing demand for healthy, natural food products. An upgraded screw press with a self-regulating pressure adjustment mechanism was developed and evaluated. Four spring types (wire diameters, 1–6 mm; maximum forces, 47.5–800 N) and screw rotation speeds ranging from 140 to 200 rpm were investigated for their effects on juice yield, compression density, internal pressure, and power consumption using sea buckthorn fruits. The results indicated that springs with higher maximum forces yielded greater juice outputs. The highest juice yield of 47% was achieved using a spring with a 3 mm wire diameter and 630 N maximum force (Spring 4) at 185 rpm. This configuration also demonstrated an optimal balance between compression density (948 kg/m3) and internal pressure (320 Pa) while maintaining the lowest power consumption (142 W). The internal pressure within the pressing chamber increased with both spring force and rotation speed. While Spring 2 generated the highest internal pressures (up to 570 Pa at 200 rpm), Spring 4 achieved moderate internal pressures, suggesting an effective pressure transmission. Spring 4 exhibited the lowest power consumption despite the high juice yields and compression densities. The study concludes that utilizing Spring 4 at 185 rpm optimizes the juice extraction efficiency while minimizing energy use. Equipment designs allowing the fine-tuning of pressure and rotation speed can significantly enhance the production efficiency in sea buckthorn juice extraction and potentially in other fruit-juice-processing applications.
Study on Vibration Characteristics of Multi-Beam Structures with Stick and Slip at Joints
Authors: Zhang Xian , Xie Yingchun , Lyu Peng , Ning Donghong , Li Zhixiong
Published: January 2025

Stick–slip phenomena may manifest at the joints during cyclic vibrations in beam structures connected by some forms of joint. This work incorporates the sticking–slip effect of joint connections into the dynamic analysis framework of multi-beam structures through changes in friction forces. The system characteristic equation is solved using the incremental harmonic balance method, the vibration characteristics of the connected structure are explored through the dynamic response, and the accuracy of the model established in this paper is verified through experiments. The equivalent stiffness and damping changes of a connecting beam under different connection states are investigated for the first time. The research indicates that the “tracking” phenomenon, induced by abrupt damping and resonance frequency variations due to low contact pressure and a low friction coefficient, leads to a relatively stable vibration response amplitude across an extended frequency range. This results in the gradual attenuation of resonance peaks within the frequency response curve, giving rise to a defined resonance frequency range. As connection stiffness diminishes, the system demonstrates characteristics of internal resonance. In addition, the influence characteristics of external excitation and connection joint position on the vibration response of multi-beam structures are also explored. This model provides an effective method for studying the vibration problems of complex beam frame structures.
Comparing 2D and 3D Feature Extraction Methods for Lung Adenocarcinoma Prediction Using CT Scans: A Cross-Cohort Study
Authors: Gouveia Margarida , Mendes Tânia , Rodrigues Eduardo M. , P. Oliveira Hélder P. , Pereira Tania
Published: January 2025

Lung cancer stands as the most prevalent and deadliest type of cancer, with adenocarcinoma being the most common subtype. Computed Tomography (CT) is widely used for detecting tumours and their phenotype characteristics, for an early and accurate diagnosis that impacts patient outcomes. Machine learning algorithms have already shown the potential to recognize patterns in CT scans to classify the cancer subtype. In this work, two distinct pipelines were employed to perform binary classification between adenocarcinoma and non-adenocarcinoma. Firstly, radiomic features were classified by Random Forest and eXtreme Gradient Boosting classifiers. Next, a deep learning approach, based on a Residual Neural Network and a Transformer-based architecture, was utilised. Both 2D and 3D CT data were initially explored, with the Lung-PET-CT-Dx dataset being employed for training and the NSCLC-Radiomics and NSCLC-Radiogenomics datasets used for external evaluation. Overall, the 3D models outperformed the 2D ones, with the best result being achieved by the Hybrid Vision Transformer, with an AUC of 0.869 and a balanced accuracy of 0.816 on the internal test set. However, a lack of generalization capability was observed across all models, with the performances decreasing on the external test sets, a limitation that should be studied and addressed in future work.
Theoretical and Numerical Simulation Research on Fire of Large-Span Spatial Structures
Authors: Sun Guojun , Zhang Xin , Wu Jinzhi , Xiao Shuo , Xue Suduo
Published: January 2025

There are obvious differences in shape and space between the large-span spatial structure and the traditional steel structure, and there will be openings at the top of the spatial structure. However, there are few studies on the fire of the spherical dome large space building with openings at the top, which makes the classical plume model inapplicable. The axial temperature of the plume centerline predicted by the traditional plume model is quite different from the real results. Therefore, this paper investigates the temperature dynamics within large-span spatial structures during large-scale fire scenarios, utilizing a combination of theoretical analysis and finite element numerical simulations. It meticulously assesses how different natural ventilation inlet areas affect both the smoke exhaust capacity and the temperature field distribution within these structures. The research expands on the traditional plume model by introducing an enhanced formula for calculating the plume center velocity, specifically designed for large-span structures with top openings. Additionally, using an improved two-region model, the paper derives a logarithmic model that describes the temperature variation as a function of vertical height within the structure. This theoretical model is then compared with numerical simulation results. The study finds that increasing the natural ventilation inlet area significantly enhances the efficiency of smoke exhaust and reduces temperatures within the fire smoke layer of large-span spatial structures. The derived temperature logarithmic curve model shows high precision in predicting the spatial temperature distribution after the fire reaches a quasi-steady state, with an average relative error of 6% between predicted and simulated temperatures, confirming its accuracy. The conclusion is of great significance to the study of fire smoke movement in large-span spatial structures. The obtained logarithmic curve model of temperature under fire provides an important basis for the fire protection design of spherical dome spatial structures under natural smoke exhaust.
Antibiofilm Activity of Protamine Against the Vaginal Candidiasis Isolates of Candida albicans, Candida tropicalis and Candida krusei
Authors: Jeyarajan Sivakumar , Kandasamy Indira , Veerapandian Raja , Jayachandran Jayasudha , Chandrashekar Shona , Natarajaseenivasan Kalimuthusamy , Chidambaram Prahalathan , Kumarasamy Anbarasu
Published: January 2025

Candida species, normally part of the healthy human flora, can cause severe opportunistic infections when their population increases. This risk is even greater in immunocompromised individuals. Women using intrauterine contraceptive devices (IUDs) are at higher risk for IUD-associated vulvovaginal candidiasis (VVC) because the device provides a surface for biofilm formation. This biofilm formation allows the normal flora to become opportunistic pathogens, leading to symptoms of VVC such as hemorrhage, pelvic pain, inflammation, itching and discharge. VVC is often linked to IUD use, requiring the prompt removal of these devices for effective treatment. This study evaluated the activity of the arginine-rich peptide “protamine” against Candida albicans, Candida tropicalis and Candida krusei isolated from IUD users who had signs of VVC. The antimicrobial activity was measured using the agar disk diffusion and microbroth dilution methods to determine the minimum inhibitory concentration (MIC). The MIC values of protamine against C. albicans, C. tropicalis and C. krusei are 32 μg mL−1, 64 μg mL−1 and 256 μg mL−1, respectively. The determined MIC of protamine was used for a biofilm inhibition assay by crystal violet staining. Protamine inhibited the biofilm formation of the VVC isolates, and its mechanisms were studied through scanning electron microscopy (SEM) and a reactive oxygen species (ROS) assay. The disruption of cell membranes and the induction of oxidative stress appear to be key mechanisms underlying its anti-candidal effects. The results from an in vitro assay support the potential use of protamine as an antibiofilm agent to coat IUDs in the future for protective purposes.
Fit Talks: Forecasting Fitness Awareness in Saudi Arabia Using Fine-Tuned Transformers
Authors: Alturayeif Nora , Alqahtani Deemah , Aljameel Sumayh S. , Almajed Najla , Alshehri Lama , Aldhuwaihi Nourah , Alhadyan Madawi , Aldakheel Nouf
Published: January 2025

Understanding public sentiment on health and fitness is essential for addressing regional health challenges in Saudi Arabia. This research employs sentiment analysis to assess fitness awareness by analyzing content from the X platform (formerly Twitter), using a dataset called Saudi Aware, which includes 3593 posts related to fitness awareness. Preprocessing steps such as normalization, stop-word removal, and tokenization ensured high-quality data. The findings revealed that positive sentiments about fitness and health were more prevalent than negative ones, with posts across all sentiment categories being most common in the western region. However, the eastern region exhibited the highest percentage of positive sentiment, indicating a strong interest in fitness and health. For sentiment classification, we fine-tuned two transformer architectures—BERT and GPT—utilizing three BERT-based models (AraBERT, MARBERT, CAMeLBERT) and GPT-3.5. These findings provide valuable insights into Saudi Arabian attitudes toward fitness and health, offering actionable information for public health campaigns and initiatives.
Effects of Customized Full-Contact Insoles Crafted with Polyester Fabric Sheets on Plantar Pressure and Gait in Hallux Valgus
Authors: Chen Hsin-Yu , Chang Chin-Kang , Wang Fu-Ting , Yen Chia-Hao , Chuang Hsiang-Chun , Wang Tsung-Yang , Chiu Fang-Yao , Peng Hsien-Te
Published: January 2025

This study investigates the benefits of innovative full-contact insoles, crafted using polyester fabric sheets of thermoplastic extruded materials, for individuals with hallux valgus-induced plantar pain. Thirty-five individuals with hallux valgus-induced foot pain were randomly allocated to either the experimental group, wearing innovative full-contact insoles 6 h daily, or the control group, using regular foot insoles, over a period of 12 weeks. Data collection occurred at baseline, and at 1 week, 2 months, and 3 months after the initial fitting. Results indicated that the innovative full-contact insoles significantly reduced anteroposterior displacement by an average of 0.9 cm (p = 0.025) and displacement area by 0.79 cm2 (p = 0.012). Gait improvements included an increase in the swing phase (36.46%, p = 0.008) and a reduction in stance phase duration (63.54%, p = 0.019). Pressure peaks at critical foot regions like the second metatarsal and medial heel were reduced by up to 39.45 kPa (p = 0.016) and 104.07 kPa (p = 0.031), while contact areas in the midfoot increased by 6.17 cm2 (p = 0.039). Foot pain decreased by an average score of 1.28 points on a 10-point scale across various measures (p = 0.041). These findings suggest that the innovative full-contact insoles effectively enhance pressure distribution and gait dynamics in patients with hallux valgus, providing a viable option for managing foot discomfort.
Integrin and Its Associated Proteins as a Mediator for Mechano-Signal Transduction
Authors: Katoh Kazuo
Published: January 2025

Mechano-signal transduction is a process in which cells perceive extracellular mechanical signals, convert them into intracellular biochemical signals, and produce a response. Integrins are cell surface receptors that sense the extracellular mechanical cues and bind to the extracellular matrix (ECM). This binding induces integrin clustering and activation. Cytoplasmic tails of activated integrins interact and induce cytoskeleton tensions via several adaptor proteins. Integrins monitor extracellular stiffness via cytoskeleton tensions and modulate ECM stiffness via downstream signaling pathways regulating the expression of genes of ECM components. Integrin-mediated mechano-transduction is very crucial for the cell as it regulates the cell physiology both in normal and diseased conditions according to extracellular mechanical cues. It regulates cell proliferation, survival, and migration. Abnormal mechanical cues such as extreme and prolonged mechanical stress result in pathological conditions including fibrosis, cancers, skin, and autoimmune disorders. This paper aims to explore the role of integrins and their associated proteins in mechano-signal transduction. It highlights the integrins and their associated proteins as targets for therapy development. Furthermore, it also presents the challenges to the targeted drug development, which can be drug resistance and cytotoxicity. It is concluded in this paper that research on integrin-mediated mechano-signal transduction and its relationship with cell physiology and pathologies will be an important step towards the development of effective therapies.
Decoding Bacterial Motility: From Swimming States to Patterns and Chemotactic Strategies
Authors: Zhuang Xiang-Yu , Lo Chien-Jung
Published: January 2025

The bacterial flagellum serves as a crucial propulsion apparatus for motility and chemotaxis. Bacteria employ complex swimming patterns to perform essential biological tasks. These patterns involve transitions between distinct swimming states, driven by flagellar motor rotation, filament polymorphism, and variations in flagellar arrangement and configuration. Over the past two decades, advancements in fluorescence staining technology applied to bacterial flagella have led to the discovery of diverse bacterial movement states and intricate swimming patterns. This review provides a comprehensive overview of nano-filament observation methodologies, swimming states, swimming patterns, and the physical mechanisms underlying chemotaxis. These novel insights and ongoing research have the potential to inspire the design of innovative active devices tailored for operation in low-Reynolds-number environments.
Therapeutic Actions of Hepatocyte Extracellular Vesicles in a Murine Model of Diet-Induced Steatohepatitis with Fibrosis
Authors: Li Xinlei , Chen Ruju , Kemper Sherri , Xu Zhaohui , Brigstock David R.
Published: January 2025

Introduction: Metabolic dysfunction-associated steatohepatitis (MASH) is a leading cause of liver failure globally and is characterized by hepatic steatosis and inflammation, which may progress to fibrosis, the severity of which is highly predictive of patient demise and death. In view of the lack of treatment options for MASH, we investigated the therapeutic properties of extracellular vesicles (EVs) from normal human hepatocytes, which we have previously been shown to alleviate toxin-mediated hepatic fibrosis in mice. Methods: C57BI/6J mice were fed a choline-deficient amino acid-defined high (60%) fat (CDAA-HF) diet for up to 12 weeks while receiving i.p. administration of EVs purified from cultured human HepG2 hepatocytes. Results: CDAA-HF diet consumption resulted in severe hepatic steatosis, increased frequency of CD45+ lymphocytes and F4/80+ macrophages, robust production of aortic smooth muscle actin (ACTA2), and deposition of interstitial collagen, as well as altered serum levels of ALT, AST, cholesterol, triglycerides, alkaline phosphatase, unconjugated bilirubin, and total protein, thus recapitulating typical MASH phenotypes. EVs administered preventively or therapeutically resulted in the restoration of serum marker levels, reduced hepatic inflammation and attenuation of collagen deposition, ACTA2 production, and expression of fibrosis-associated genes. HepG2 EVs contained 205 miRs and, among the 30 most abundant miRs, seven (miRs-423-5p, -483-5p, -191-5p, -148a-3p, -423-3p, -92a-3p, -122-5p) are predicted to directly target fibrosis-related genes (collagens, ACTA2, MMPs, and TIMPs). Conclusions: Hepatocyte EVs are therapeutic in a mouse model of diet-induced steatohepatitis with fibrosis. Further studies of hepatocyte EVs or their cargo components as novel therapeutics for MASH in humans are warranted, including treatment of fibrotic stages, which are associated with clinical demise and are predictive of patient death.
Comprehensive Analysis Reveals Midnolin as a Potential Prognostic, Therapeutic, and Immunological Cancer Biomarker
Authors: Zhang Xin-Guo , Li Wen-Ting , Jin Xin , Fu Chuang , Jiang Wen , Bai Jie , Shi Zhi-Zhou
Published: January 2025

Background/Objectives: MIDN (midnolin) is newly discovered method for critically regulating a ubiquitin-independent proteasomal degradation pathway. This study aims to examine the expression, prognostic value, genomic changes, interacting proteins, methylation status, and correlations with the tumor immune microenvironment of MIDN in various cancers. Methods: The GTEx, Depmap, GEPIA2, and Kaplan–Meier Plotter databases are applied to evaluate the MIDN level in tumor and normal tissues and the MIDN prognostic value in cancers. The genetic alterations of MIDN in cancers are investigated using the cBioPortal database. The STRING, GeneMANIA, DAVID, and Human Protein Atlas are harnessed to identify and analyze MIDN-interacted proteins. The Sangerbox 3.0 platform (a pan-cancer analysis module) is used to measure the correlations between the MIDN level and the tumor immune microenvironment, stemness, immune cell infiltration, tumor mutational burden, immune checkpoint genes, and RNA modification genes. Immunofluorescence, qRT-PCR, and Western blotting assays were used to evaluate the biological roles of MIDN in breast and gastric cancer cells. Results: MIDN expression was dysregulated in many cancers and associated with prognosis in several cancers, such as esophageal cancer. MIDN was mutated in 1.7% of cancers, and deep deletion was the dominant mutation type. NR4A1, PSMC1, and EGR1 were selected as MIDN-interacted proteins, and these four molecules were co-expressed in pancreatic cancer, liver cancer, urothelial cancer, melanoma, and breast cancer. MIDN expression was significantly correlated with the infiltration of CD8+ T cell, CD4+ T cell, B cell, macrophage, neutrophil, and DC both in prostate adenocarcinoma and liver hepatocellular carcinoma. The MIDN level was correlated with several immune checkpoint genes, such as VEGFA, and RNA modification genes such as YTHDF1, YTHDF2, YTHDF3, and YTHDC1 in cancers. Furthermore, in breast cancer cells, the downregulation of MIDN suppressed the colony formation abilities and lessened cell-cycle-associated and stemness-associated genes; in gastric cancer, the knockdown of MIDN diminished the mRNA levels of Nanog and LDHA. Strikingly, silence of MIDN upregulated FTO protein expression in both breast and gastric cancer cells. Conclusions: Our findings demonstrate the expression, prognostic value, mutation status, interacting proteins, methylation status, and correlations with the tumor immune microenvironment of MIDN. MIDN will be developed as a potential therapeutic target and a prognosis biomarker.
A Standardized Extract of Zingiber officinale Roscoe Regulates Clinical and Biological Outcomes in Two Different EAE Mouse Models
Authors: Borgonetti Vittoria , Governa Paolo , Morozzi Martina , Sasia Chiara , Videtta Giacomina , Biagi Marco , Galeotti Nicoletta
Published: January 2025

Background/Objectives: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by demyelination and neuronal damage. Current MS therapies are unsatisfactory, and new therapies are encouraged. A correlation between nutritional intake and MS has been speculated. Supplementation of approved immunomodulatory therapy with herbal medicines possessing antioxidant and anti-inflammatory activities could provide benefits to MS patients. Ginger is one of the most widely consumed dietary supplements in the world, commonly used in traditional medicine. Studies demonstrated that ginger may also be beneficial in the management of neurodegenerative diseases. The aim of this study is to investigate the MS therapeutic potential of ginger. Methods: A standardized Zingiber officinale Roscoe extract (ZOE) was orally administered for 14 days. Two experimental autoimmune encephalomyelitis (EAE) models in mice were used. The PLP139-151-EAE relapsing-remitting model and MOG35–55-EAE chronic model. Clinical score, von Frey, hot plate, and rotarod tests were used for behavioral tests. ELISA and Western blotting were used to measure cytokines levels. Evans Blue content was determined spectrophotometrically. Results: ZOE attenuated motor disability and pain hypersensitivity in both models had no effect on body weight loss. ZOE reduced the blood–brain barrier (BBB) permeability in the PLP-EAE models and reduced levels of circulating cytokines (Il-6, IL-17) in the MOG-EAE model. ZOE attenuated spinal cytokines overexpression in both models. Conclusions: ZOE improves EAE symptoms and attenuates the proinflammatory response in both models, representing a promising nutraceutical support to the conventional therapeutic approach to MS.
NELL2-PAX7 Transcriptional Cascade Suggests Activation Mechanism for RAD52-Dependent Alternative Lengthening of Telomeres During Malignant Transformation of Malignant Peripheral Nerve Sheath Tumors: Elongation of Telomeres and Poor Survival
Authors: Lee Jungwoo , Choi Eunji , Kim Hyoju , Kim Young-Joon , Kim Seung Hyun
Published: January 2025

Background: In eukaryotes with a double-stranded linear DNA genome, the loss of terminal DNA during replication is inevitable due to an end-replication problem; here, telomeres serve as a buffer against DNA loss. Thus, the activation of the telomere maintenance mechanism (TMM) is a prerequisite for malignant transformation. Methods: We compared neurofibroma (NF, benign) and malignant peripheral nerve sheath tumors (MPNSTs) occurring in the same patient with type 1 neurofibromatosis, where each NF–MPNST pair shared the same genetic background and differentiation lineage; this minimizes the genetic bias and contrasts only those changes that are related to malignant transformation. A total of 20 NF–MPNST pairs from 20 NF1 patients were analyzed. Whole-transcriptome sequencing (WTS) was conducted to profile the transcriptional relationship, and whole-genome sequencing (WGS) was performed to measure the telomere length. Results: We identified 22 differentially expressed genes (DEGs) during the malignant transformation of MPNSTs. Among them, NELL2 activated PAX7, which sequentially activated RAD52, the recombinase of RAD52-dependent alternative lengthening of telomeres (ALT). RAD52 elongated MPNSTs–telomeres (p = 0.017). Otherwise, neither NELL2 nor PAX7 affected telomere length (p = 0.647 and p = 0.354, respectively). RAD52 increased MPNSTs–telomeres length, independently of NELL2 and PAX7 in multiple analyses (p = 0.021). The group with increased telomere length during the malignant transformation showed inferior overall survival (OS) (HR = 3.809, p = 0.038) to the group without increased telomere length. Accordingly, the group with increased PAX7 showed inferior OS (HR = 4.896, p = 0.046) and metastasis-free survival (MFS) (HR = 9.129, p = 0.007) in comparison to the group without increased PAX7; the group with increased RAD52 showed inferior MFS (HR = 8.669, p = 0.011) in comparison to the group without increased RAD52. Conclusions: We suggest that the NELL2-PAX7 transcriptional cascade activates RAD52-dependent ALT to increase telomere length during the malignant transformation of MPNSTs, resulting in a poor prognosis.
Dynamic Response of Train–Ballastless Track Caused by Failure in Cement–Asphalt Mortar Layer
Authors: Chen Xicheng , Pei Yanfei , Liu Kaiwen
Published: January 2025

Cement–asphalt (CA) mortar voids in earth’s structure are prone to inducing abnormal vibrations in vehicle and track systems and are more difficult to recognize. In this paper, a vehicle–ballastless track coupling model considering cement–asphalt mortar voids is established and the accuracy of the model is verified. There are two main novel results: (1) The displacement of the track slab in the ballastless track structure is more sensitive to the void length. Voids can lead to blocked vibration transmission between the ballastless track slab and concrete base. (2) The wheel–rail vibration acceleration is particularly sensitive to voids in cement–asphalt mortar, making the bogie pendant acceleration a key indicator for detecting such voids through amplitude changes. Additionally, the train body pendant acceleration provides valuable feedback on the cyclic characteristics associated with single-point damage in the cement–asphalt mortar, thereby enhancing the accuracy of dynamic inspections for vehicles. In the sensitivity ordering of the identification indexes of voids, the bogie’s vertical acceleration in high-speed trains > the nodding acceleration of the bogie > the vehicle’s vertical acceleration. Adaptive suspension parameters can be designed to accommodate changes in track stiffness.
Latent Class Analysis of Discrimination and Social Capital in Korean Public Rental Housing Communities
Authors: Kim Sungeun , Jeon Seran
Published: January 2025

This study explored typologies among residents of South Korean public rental housing, focusing on their experiences of discrimination and social capital. Latent class analysis (LCA) was applied to data from 4683 individuals in the 2021 Seoul Public Rental Housing Panel Survey. Four distinct groups were identified: ‘Group Seeking Friendly Neighbor Relationships’, ‘Group Accepting Losses’, ‘Group with High Social Capital’, and ‘Group Indifferent to Neighbors’. The findings revealed that while discrimination was widespread, certain groups exhibited strong social capital. Notably, the ‘Group Accepting Losses’ showed the highest willingness to help neighbors despite facing significant discrimination, while the ‘Group with High Social Capital’ displayed high levels of neighbor trust and mutual support. These results challenge traditional views by showing that social capital can thrive even in the presence of discrimination. This study suggests that policies aimed at addressing discrimination in public rental housing should focus not only on physical integration but also on fostering social connections to enhance community cohesion and reduce mental health issues among residents.
Reinforcing the Flexural Fracture Zone in the Xiangjiaba Hydropower Station by Simultaneously Applying Wet-Milling Cement and Chemical Compound Grouting
Authors: Zhang Da , Wei Tao , Tang Wenjian , Han Wei , Wu Yan , Liao Lingmin
Published: January 2025

This paper reports the results of a full-scale field test that was conducted to assess the performance of the use of wet-milling cement and chemical compound grouting in the same hole to reinforce a flexural fracture zone. Wet-milling cement and chemical compound grouting methods were used to treat a layer of the flexural fracture zone with a thickness of 19 m. The procedures of the cement–chemical compound grouting method were described in detail, and the results of the normal water pressure test, fatigue water pressure test, failure water pressure test, and shear wave velocity test suggested that the working effects in the epoxy testing area were better than those in the acrylic acid salt test area, which further indicated that the cement–chemical compound grouting method was feasible. In addition, the improvement mechanism of the cement–chemical compound grouting technology was studied; this method is beneficial for solving the problem of the reinforcement effect not being ideal in practical engineering and further improving the compactness of dam structures.
Influence of the Objective Function in the Dynamic Model Updating of Girder Bridge Structures
Authors: Di Re Paolo , Vangelisti Iacopo , Lofrano Egidio
Published: January 2025

In the context of model updating of bridge structures, dynamic approaches are currently dominant. This is mainly due to the opportunity of performing dynamic tests under environmental and traffic loadings, without putting the bridges out of service. Several techniques have been proposed in the literature to control and address the relevant model updating workflow. These methods typically consider the structural frequencies, or a combination of frequencies with vibration modes. Dissipative properties are, on the contrary, more rarely considered in updating procedures, given their strong dependence on the amplitude of the vibrations and on the type of forcing load. In this work, six ruling objective functions are considered for the dynamic model updating of girder bridge structures. The first one, taken from the literature, is a widely used function based on discrepancies among numerical and experimental frequencies. Two additional functions, also derived from the existing literature, are subsequently considered: one focuses on vibration modes, utilizing the Modal Assurance Criterion (MAC), and the other incorporates both structural frequencies and mode shapes, deploying the Modal Flexibility Matrix (MFM). Three novel objective functions are introduced, which are adaptations of the previously mentioned ones, with alternative applications of MAC and MFM. These six functions are analyzed and discussed through two comprehensive experimental case studies, in which the relative weights of the specific function terms are also investigated. A quantitative selection criterion is proposed and examined in order to choose the most suitable objective function based on identifiability. The method implementation, leveraging second-order derivatives, is executed via a finite difference scheme.
Enjoying Your Neighbourhood During the COVID-19 Pandemic? A Hong Kong Study on Housing-Related Anti-Social Behaviour
Authors: Yau Yung
Published: January 2025

Studies have established a negative correlation between residents’ perceptions of anti-social behaviours (ASBs) and their sense of community (SOC) within their neighbourhoods. Yet, whether their relationship persisted during the COVID-19 pandemic was under-researched, when daily life significantly changed. Theoretically, the pandemic’s impact on ASB and SOC was multifaceted, as lockdowns and work-from-home arrangements led to an increased time spent at home, potentially exacerbating neighbour nuisances and vulnerability to ASB, but also fostered opportunities for community building. Residents spent more time in their neighbourhoods engaging in neighbourly interactions and mutual aid initiatives, potentially strengthening SOC. To investigate these contrasting effects, this study analysed data from two questionnaire surveys conducted among private housing residents in Hong Kong before and after the onset of the pandemic. It finds that perceived ASB seriousness and SOC levels increased during the pandemic, yet the negative correlation between ASBs and SOC persisted. This research contributes to the literature by exploring the pandemic’s effects on SOC and ASB in high-density, high-rise contexts and expanding beyond noise nuisances to include various unacceptable behaviours in assessing ASBs. The paper concludes with policy implications and outlines a future research agenda focusing on the interplay between ASB control and SOC development in pandemic scenarios.
Shorter Time to Biopsy of Patients with Head and Neck Squamous Cell Carcinoma During the COVID-19 Pandemic in Hungary
Authors: Szabó Éva , Kopjár Eszter , Rumi László , Bellyei Szabolcs , Zemplényi Antal , Mátyus Emese , Édes Eszter , Girán János , Kiss István , Szanyi István , Pozsgai Éva
Published: January 2025

Background/objectives: The goal of this investigation was to compare the time to biopsy (TBI) and time to treatment (TTI) for head and neck squamous cell carcinoma (HNSCC) patients before and during the COVID-19 pandemic and to examine the effect of demographic and clinical characteristics on these intervals. Methods: Our retrospective study at a large regional Hungarian cancer center analyzed data from patients aged 18 or older diagnosed with HNSCC between 1 January 2017 and 15 March 2020 (pre-COVID-19 period) and 16 March 2020 to 13 May 2021 (COVID-19 period). We calculated the time from initial physician contact to biopsy (TBI) and from biopsy to treatment initiation (TTI) and performed descriptive and exploratory statistical analyses. Results: The median TBI decreased significantly (6 vs. 3 days; p = 0.008), while the median TTI was not affected significantly (28 vs. 29 days; p = 0.972) pre-pandemic and during the pandemic, respectively. Residence in a village was linked to a significant reduction in median TBI during the pandemic (p = 0.000), coinciding with a higher proportion of rural patients diagnosed with oral cavity/oropharyngeal cancers during the pandemic (50.3% pre-pandemic vs. 67.4% during pandemic, p = 0.044). Median TTI decreased significantly during the pandemic for patients with laryngeal tumors (27.5 vs. 18.5 days; p = 0.012). Conclusions: Our study, one of a few from this region, provides insights into HNSCC patient waiting times. Improvement in TBI likely resulted from the availability of telemedicine, reduced diagnostic demands from non-cancer patients, and an increased incidence of oral cavity/oropharyngeal cancer among rural patients.
Ultra-High Dose Oral ω3 Eicosapentaenoic Acid (EPA), Docosahexaenoic Acid (DHA), or Oxidation-Resistant Deuterated DHA Block Tumorigenesis in a MYCN-Driven Neuroblastoma Model
Authors: Patel Vishwa , Li Yan Ning , Benhamou Lorraine-Rana E. , Park Hui Gyu , Raleigh Mariya , Brenna J. Thomas , Powers John T.
Published: January 2025

Background/Objectives: Neuroblastoma is a genetically diverse, highly metastatic pediatric cancer accounting for 15% of childhood cancer deaths despite only having ~8% of childhood cancer incidence. The current standard of care for high-risk diseases is highly genotoxic. This, combined with less than 50% survival in high-risk diseases and an abysmal 5% survival in relapsed cases, makes discovering novel, effective, and less toxic treatments essential. Methods: A prophylactic syngeneic mouse model was used to test high-dose lipid-mediator highly unsaturated fatty acids on tumorigenesis. Wildtype mice were gavaged with 12.3–14.6 g/d (adult human equivalent) omega-3 EPA, DHA, or oxidation-resistant bis allylic deuterated DHA (D-DHA) and 4.6–6.0 g/d arachidonic acid (ARA). At seven days, MYCN-expressing murine neuro-2a cells syngeneic to the gavaged mice were injected subcutaneously. Oral gavage continued for 10–20 d post-injection when tumors and tissues were harvested. Results: Fifty percent of control (not gavaged) animals form tumors (4/8) at about 10 d. High-dose DHA, D-DHA, and EPA block tumor formation completely in n = 8 or 10 animals. In contrast, ω6 arachidonic acid (4.6–6.0 g/d) enhances tumor formation (6/10 tumors) and reduces latency (5.5 to 10 days) compared to the control. The co-delivery of ARA and EPA results in a reduced tumor burden analogous to the control group, suggesting that EPA directly opposes the mechanism of ARA-mediated tumor formation. DHA acts through a non-oxidative mechanism. Conclusions: Sustained high-dose ω3 (weeks/months) is safe and well-tolerated in humans. These results suggest that ω3 DHA and EPA delivery at ultra-high doses may represent a viable low-toxicity therapy for neuroblastoma.
Preoperative Diaphragm Muscle Atrophy Increases the Likelihood of Postoperative Pulmonary Complications After Lung Cancer Resection: A Pilot Study
Authors: Kocjan Janusz , Rydel Mateusz , Czyżewski Damian , Adamek Mariusz
Published: January 2025

Background/Objectives: Various conditions contribute to the development of postoperative pulmonary complications (PPCs) following thoracic surgery. In this study, the aim was to investigate whether preoperative diaphragm dysfunction is associated with an increased risk of PPCs after lung cancer resection. Methods: We prospectively examined 45 patients scheduled for video-assisted thoracoscopic surgery (VATS) lobectomy or open thoracotomy. Relevant clinical data were retrieved from hospital database records, while diaphragm muscles were assessed using ultrasound. Results: Our results demonstrated that preoperative diaphragm muscle atrophy was significantly associated with a higher risk of developing PPCs compared to patients with normal diaphragm thickness. Diaphragm atrophy was also linked to prolonged hospital stays. Additionally, we observed a moderate correlation between expiratory diaphragm thickness and the number of PPCs. Conclusions: Low diaphragm expiratory thickness is associated with postoperative pulmonary complications after lobectomy for lung cancer. Importantly, unlike other predictive factors such as age, COPD, or smoking, diaphragmatic atrophy is a modifiable risk factor that can potentially be addressed through early therapeutic intervention.
Development and Validation of an HPLC-FLD Method for the Determination of Pyridoxine and Melatonin in Chocolate Formulations—Digestion Simulation Study
Authors: Kamaris Georgios , Mitsiou Vasiliki-Panagiota M. , Chachlioutaki Konstantina , Almpani Sofia , Markopoulou Catherine K.
Published: January 2025

The undeniable value of sleep is commonly accepted and essential for all aspects of human existence. The aim of the present study was to develop and validate an applicable and sensitive HPLC-FLD method for the quantitative determination of pyridoxine (PYR) and melatonin (MEL) in a chocolate formulation that is suitable for the treatment of insomnia. The proposed chromatographic process was performed on an Aqua column (250 mm × 4.6 mm, 5 mm) at 45 °C, with a mobile phase consisting of two solvents (A, water with 0.2% formic acid; and B, acetonitrile with 0.2% formic acid) in a gradient elution and with a flow rate of 1.2 mL/min. Method validation was carried out according to ICH specifications in terms of linearity (R2 > 0.999), precision, and repeatability (%RSD < 2). The limits of detection (LODs: PYR = 0.09 ng/mL; MEL = 0.92 ng/mL) and quantification (LOQs: PYR = 0.29 ng/mL; LOQMEL = 3.04 ng/mL) for both analytes were also determined. A mixture D-Optimal methodology of experimental design technique was applied for the quantitative recovery of the two APIs from chocolate (% recovery > 97.4%). Finally, to simulate the digestion process of the new preparation, three phases in vitro, a digestion method was applied. According to the study, 73.8% of pyridoxine and 55.9% of melatonin (on the indicated dose) were released from the formulation and ended up in the intestine.