bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcome of Xenografted Fetal Porcine Pancreatic Tissue is Superior in Inbred Scid (C.B-17/Icr-scid/scid) Compared to Outbred Nude (CD-1-nu/nu) Mice
Authors: Tuch Bernard E. , Casamento Frances M.
Published: June 2017

Nude mice are used as recipients of foreign tissue because of their inability to reject these grafts. Our experience has been that there is variable rejection of fetal porcine insulin-producing tissue transplanted into CD-1 (athymic) outbred nude mice. To examine the suitability of this line of nude mouse as a recipient of the tissue, fetal porcine pancreas was grafted either into these outbred animals or into an inbred mutant strain of mice, the more immunocompromised severe combined immunodeficient (scid) mouse. Eight weeks after transplantation grafts were recovered from recipients and assayed for insulin content. Mean insulin levels were not significantly different between the two groups of mice, but a wider range of values was obtained from grafts recovered from nude (CD-1-nu/nu) mice. Reversal of diabetes in hyperglycemic recipients was achieved in 4 of 8 nude mice and 8 of 8 scid (C.B-17/Icr-scid/scid) mice. The time taken to achieve this was longer in the nudes than the scid mice, 121 ± 12 vs. 44 ± 2 days, the grafts increasing in size at a slower rate in the nude mice. Time taken for the weight of the grafts to double in size was 94 ± 17 vs. 32 ± 1 days, respectively. Histologically the grafts in the scid mice contained mostly epithelial cell clusters, a majority of which were insulin containing. In the nude mice that achieved normoglycemia, a similar pattern was observed and, as well, there was a localized lymphoid infiltrate. In those nude mice that remained diabetic fibrous tissue predominated together with a lymphoid infiltrate. In summary, fetal porcine pancreatic tissue grows and develops more efficiently when xenografted into scid rather than outbred nude mice.
A Preliminary Study of the Activation of Endogenous Pancreatic Exocrine Enzymes during Automated Porcine Islet Isolation
Authors: White S. A. , Djaballah H. , Hughes D. P. , Roberts D. L. , Contractor H. H. , Pathak S. , London N. J. M.
Published: June 2017

The activation of endogenous pancreatic enzymes during automated pancreas digestion may be detrimental to islet isolation. In this report we assessed the activation of trypsin, chymotrypsin, elastase, carboxypeptidases A and B, phospholipase A2, and lipase using a porcine model. Four islet isolations were examined. Duplicate aliquots were taken from the automated circuit at 5-min time intervals up to the completion of pancreas digestion (approx 60 min). One aliquot was activated in vitro with exogenous trypsin in order to convert the enzymes into their active non-“proform,” with the exception of trypsinogen, which was activated with exogenous enterokinase. This was done to assess the percentage activation of each individual enzyme (total potentially activatable enzyme release). The extent of activation between isolations was extremely variable. During the closed (recirculating) circuit phase of pancreas digestion there were both gradual and rapid increases in the levels of enzymes released. Peak activity of enzyme activation varied from 13 to 30 min; similarly, total potentially activatable peaks occurred between 13 and 38 min. Lipase and carboxypeptidase B showed greater than 70% activation, chymotrypsin, carboxypeptidase A, and phospholipase A2between 50% and 70% activation, and trypsin and elastase less than 20%. There were up to 30-fold differences between the four islet preparations. In summary, it is unlikely that poor islet yields are soley explained by variations between collagenases; the variable activation of endogenous pancreatic exocrine enzymes is also likely to be influential to porcine islet yields.
Analysis of the Th1/Th2 Paradigm in Transplantation: Interferon-γ Deficiency Converts Th1-Type Proislet Allograft Rejection to a Th2-Type Xenograft-Like Response
Authors: Simeonovic Charmaine J. , Townsend Michelle J. , Karupiah Gunasegaran , Wilson J. Dennis , Zarb Jodie C. , Mann David A. , Young Ian G.
Published: June 2017

The rejection mechanisms for fetal proislet allografts and pig proislet xenografts in mice are characterized by different intragraft cytokine mRNA profiles and cellular responses. Allograft rejection is predominantly CD8 T-cell-dependent and is associated with a Th1-type cytokine pattern (i.e., IFN-γ, IL-2 but no IL-4 or IL-5 mRNA). In contrast, xenograft rejection is CD4 T-cell-dependent and is accompanied by a strong Th2-type response (i.e., enhanced expression of IL-4 and IL-5 mRNA) and by marked eosinophil accumulation at the graft site. We have now examined and compared the regulatory role of IFN-γ in both proislet allograft and xenograft rejection processes. The histopathology and intragraft cytokine mRNA profile of BALB/c (H-2d) proislet allografts were examined in IFN-γ-deficient and wild-type C57BL/6J recipient mice. The survival of pig proislet xenografts was also assessed in IFN-γ –/– and wild-type hosts. Both proislet allografts and xenografts were acutely rejected in IFN-γ –/– and wild-type mice. Unlike the conventional allograft reaction, which lacks eosinophil infiltration, the rejection of proislet allografts in IFN-γ-deficient hosts correlated with intragraft expression of IL-4 and IL-5 mRNA (i.e., a Th2-type response) and eosinophil recruitment. The rejection of proislet allografts and xenografts can therefore occur by IFN-γ-independent pathways; IFN-γ, however, regulates the pathology of the allograft reaction but not the xenograft response. The immune destruction of proislet allografts is not prevented by Th2 cytokine gene expression; instead, the latter correlated with the recruitment of unconventional inflammatory cells (eosinophils), which may play an accessory role in effecting graft injury. Significantly, the Th1-to-Th2-like switch resulted in the novel conversion of an allograft rejection reaction into a xenograft-like rejection process.
Bioartificial Endocrine Pancreas (Bio-AEP) for Treatment of Diabetes: Effect of Implantation of Bio-AEP on the Pancreas
Authors: Hirotani Sachiko , Eda Reiko , Kawabata Takako , Fuchinoue Shohei , Teraoka Satoshi , Agishi Tetsuzo , Ohgawara Hisako
Published: June 2017

Recently, we described a diffusion chamber for a bioartificial endocrine pancreas (Bio-AEP). Pancreatic islet cells in the Bio-AEP device were isolated from the immune system of the host by an artificial barrier, while nutrients, electrolytes, oxygen, and bioactive secretory products were exchanged across this barrier. This experiment was designed to evaluate whether the diffusion chamber could be useful as a Bio-AEP in the treatment of diabetes. Six streptozotocin (STZ)-induced diabetic rats each received a diffusion chamber containing 8 × 106 MIN6 cells as a xenograft Bio-AEP. In the STZ diabetic rats with Bio-AEPs, a return to normoglycemia was observed up to 30 weeks after implantation, without the use of any immunosuppressant. A gradual increase in the body weight of the rats was also observed. In three STZ diabetic rats, diffusion chambers without MIN6 cells were implanted as a sham operation. The fasting blood glucose levels in these three rats remained higher than 600 mg/dl, after implantation, and they lost weight. Thirty-five weeks after implantation, the pancreata were removed from the rats that underwent xenoimplantation, those that had the sham operation, and the normal control rats. In the sham-operated animals, the exocrine tissues of the pancreata were vacuolated and pancreatic B cells were not seen in the islets. In contrast, in the pancreata from the xenoimplantation, the exocrine tissues were normal, and a few pancreatic B cells were seen in the islets. These results indicated that xenoimplantation using the Bio-AEP might retard the progress of diabetes.
Beneficial Effects of Immunoisolated Fetal and Neonatal Pig Liver Fragments on Acute Liver Failure in a Large Animal
Authors: Kanai Nobuyuki , Hagihara Masao , Nagamachi Yukio , Tsuji Kimiyoshi
Published: June 2017

Xenogeneic cell (fragment) transplantation may be used as an interim therapy until the organ allotransplanation. Immunologic rejection, however, constitutes the major hurdle. To overcome this problem, “xeno” fetal and neonatal liver fragments (FLF, NLF) were encapsulated into separate micropore devices that protect them from immunological attack by the recipient. The FLF or NLF were then transplanted into beagles with hepatic failure to observe their biological effects. In Experiment 1 (n = 5) beagles were injected IV with D-galactosamine (D-gal, 1.0 g/kg) on day 0 and then received FLF grafts (0, 0.3, 0.8, 1.0, 2.0 g/kg). In Experiment 2 (n = 6) beagles received NLF grafts (1.8 g/kg) and on the following day were injected with D-gal (1.0 g/kg). In Experiment 1 only the high dose of xeno-FLF (2.0 g/kg) decreased the elevated ALT (GPT) and T. Bil. levels. Histologic examination showed that some of the hepatocytes of the host liver survived only in the high-dose graft. In Experiment 2, at 36 and 48 h after D-gal injection, the transplanted group had a significantly lower AST (GOT) level than the control. The grafted NLF survived for 14 days, according to histologic examinations. Thus, encapsulated FLF and NLF xenotransplantation can prevent liver dysfunction in a large animal hepatic failure model.
Porcine Embryonic Brain Cell Cytotoxicity Mediated by Human Natural Killer Cells
Authors: Sumitran Suchitra , Anderson Per , Widner Håkan , Holgersson Jan
Published: June 2017

Intracerebral transplantation of porcine embryonic dopamine-producing neurons has been suggested as a method to treat patients with Parkinson's disease. Even though the brain is an immunologically privileged site, neuronal xenografts are usually rejected within a few weeks. T cells are important for this process, but the exact cellular events leading to rejection are poorly characterized. Brain cells from ventral mesencephalon of 26–27-day-old pig embryos were used as target cells in flow cytometry-assessed cytotoxicity assays using non- and IL-2-activated CD3–CD16+CD56+human natural killer (NK) cells as effector cells. The ability of human NK cells to kill pig embryonic brain cells by antibody-dependent cellular cytotoxicity (ADCC) in the presence of nondepleted and anti-Galα1,3Gal antibody-depleted human blood group AB serum (AB serum) was evaluated using the same assay. Both nondepleted and anti-Galα1,3Gal antibody-depleted AB serum could mediate ADCC of pig embryonic VM cells when human NK cells were used as effector cells. Nonactivated NK cells did not show any direct cytotoxic effect on freshly isolated VM cells, whereas IL-2-activated NK cells killed approximately 50% of the VM cells at an effector-to-target ratio of 50:1 in a 4-h cytotoxicity assay. Activation of VM cells by TNF-α did not change their sensitivity to human NK cell cytotoxicity. Human NK cells may thus contribute to a cellular rejection of pig neuronal xenografts by ADCC, or following IL-2 activation, by a direct cytotoxic effect.
Viability of Periosteal Tissue Obtained Postmortem
Authors: O'driscoll Shawn W. , Meisami Borna , Miura Yasushi , Fitzsimmons James S.
Published: June 2017

Periosteal autografts have the potential to regenerate articular cartilage defects, but this potential is limited by the patient's age. Allograft transplantation from a young donor to an older recipient might bypass this limitation. The effect of the time delay, between death and harvesting of a periosteal graft, on the chondrogenic potential of periosteum is important not only for transplantation but also for studies dealing with tissues retrieved postmortem (i.e., including the periosteal explant model). The purpose of this study was to investigate the chondrogenic potential of periosteum obtained postmortem and a possible beneficial effect of hypothermia. Thirty NZ white rabbits (2 months old) were sacrificed and stored at room temperature or 4°C for 0, 4, 6, 8, 12, 16, 18, or 24 h. Periosteal explants were then obtained and a standard cartilage yield assay performed by culturing them for 6 weeks using the periosteal organ culture model as previous published. TGF-β1 (10 ng/ml) was added for the first 14 days of culture. Histochemical analysis and quantitative collagen typing were performed. In the explants from the animals kept for 4 h at room temperature growth and chondrogenesis were dramatically reduced. Little or no chondrogenesis was seen in expiants from rabbits maintained at room temperature after 4–8 h (or more) postmortem. Cooling the rabbits to 4°C partially prevented this loss of viability and continued to do so for 24 h. Even storage at 4°C did not eliminate the decrease in chondrogenic potential, though it did permit partial preservation of chondrogenic potential. If periosteum is to be used for allograft transplantation, or if it is used for experimental study, its viability must be assured. This is best accomplished by harvesting it immediately postmortem. Preservation techniques, cryopreservation, or hypothermia might be useful in preserving periosteal chondrogenic potential.
The Effects of Microencapsulation on Pancreatic Islet Osmotically Induced Volumetric Response
Authors: Woods Erik J. , Liu Jun , Zieger Michael A. J. , Lakey Jonathan R. T. , Critser John K.
Published: June 2017

Microencapsulation of pancreatic islets has been proposed as a means to prevent allograft rejection and to protect islets during cryopreservation. The aim of this study was to investigate: 1) the effects of the cryoprotectants (CPAs) dimethyl sulfoxide (DMSO) and ethylene glycol (EG) on the volume of Ca2+ alginate microcapsules, and 2) the effects of microencapsulation on the volumetric response of human and canine pancreatic islets during CPA equilibration. Stock sodium alginate with a high mannuronic acid content (HM) or a high guluronic acid content (HG) was used to generate empty capsules (mean diameter 200 μm) with an electrostatic generator. The capsules were held in place by a holding pipette system and videotaped during the addition of 2 or 3 M CPA at 22°C. Islets (isolated from human cadaveric donors and mongrel dogs and then cultured overnight at 37°C) were encapsulated in alginate (HM), loaded into a microperfusion chamber, and the change in islet volume was videotaped after exposure to the same CPAs and concentrations. These were compared to the volume responses of nonencapsulated islets. Images were analyzed using a computerized image analysis system and the data were analyzed using ANOVA. HG microcapsules showed a significant (p < 0.05) increase in volume following exposure to EG but not to DMSO. HM microcapsule volume did not change significantly following exposure to either EG or DMSO and was therefore chosen as the substrate for islet encapsulation. Free, nonencapsulated canine and human islets responded to the osmotic challenge of the 2 M DMSO by shrinking to 70.00 ± 1.04% (mean ± SEM) and 70.11 ± 1.05%, and in 2 M EG to 72.89 ± 1.93% and 69.33 ± 1.38%, respectively, of the isotonic volume before returning to the original cell volume. Exposure to 3 M DMSO or EG resulted in a further dehydration to 65.89 ± 0.91.% and 67.67 ± 1.91% for canine and 62.22 ± 0.66.% or 65.89 ± 1.30% for human islets. Minimum volumes were reached within 30–40 s after exposure to the cryoprotectant. Encapsulated human islets reached 86.88 ± 1.47% of their original volume in 2 M and 80.33 ±0.89% in 3 M DMSO, and 87.33 ± 1.86% in 2 M and 82.80 ± 1.57% in 3 M EG. This volume change was significantly less (p < 0.01) than that observed in corresponding free islets. Encapsulated canine islets reached 83.67 ± 2.13% of their original volume in 2 M and 78.22 ± 0.95% in 3 M DMSO, and 85.44 ± 1.92% in 2 M and 78.11 ± 2.01% in 3 M EG. As with human islets, this was significantly different than free islets (p < 0.01). These minimal volumes were reached within 30–50 s. These results demonstrate that there are cryoprotectant and alginate-specific interactions and that microencapsulation modulates the degree of osmotically induced shrinkage of islets. The development or modification of existing cryopreservation protocols to improve postcryopreservation recovery or function must account for these factors.
Lessons from in Vitro Perifusion of Pancreatic Islets Isolated from 80 Human Pancreases
Authors: Bertuzzi F. , Garancini P. , Socci C. , Nano R. , Taglietti M. V. , Santopinto M. , Di Carlo V. , Davalli A. M.
Published: June 2017

We report the average insulin response to acute glucose measured by in vitro perifusion of pancreatic islets isolated from 80 consecutive human organs. Different perifusion parameters were considered [basal release, stimulation index (SI), time to peak, incremental area under the curve Δ-AUCa)], and the correlation among them was determined. SI positively correlated with Δ-AUCa (p < 0.001, r = 0.80) while negatively with time to peak (p < 0.05, r = −0.23). We also evaluated several variables of the isolation procedure that might affect responsiveness to glucose by human islets. Sex and age of pancreas donors, cold ischemia time, duration of the digestion, collagenase concentration, and lot characteristics (collagenase, trypsin, clostripain, and proteases activity), and final islet yield were considered. Multivariate regression analysis showed only an independent association between SI and the concentration of collagenase (p = 0.01).
The Wehrmacht and National Socialist Military Thinking
Authors: Stahel David
Published: June 2017

This article argues that Nazi ideology had a profound impact upon the German officer corps in the Second World War not just for their well-established complicity in criminal activity, but also in their approach to warfare. This article demonstrates that Nazi ideology radicalized pre-existing notions of the German military ethos and conceptions of war, leading to an often irrational world view in which impractical, and even impossible, military undertakings could be ‘rationally’ explained, accepted, and understood. At the same time, seventy years after the war, a National Socialist influence upon German military thinking has seldom been discussed in the proliferation of Anglo-American operational histories.
Long non-coding RNA urothelial carcinoma–associated 1 as a tumor biomarker for the diagnosis of urinary bladder cancer
Authors: Wang Zichun , Wang Xiaoxiong , Zhang Daming , Yu Yongchun , Cai Licheng , Zhang Cheng
Published: June 2017

To investigate the diagnostic value of urothelial carcinoma–associated 1 as a urine biomarker in urinary bladder cancer patients by performing a comprehensive meta-analysis. A comprehensive literature search was conducted by the databases PubMed, Embase, Cochrane Library, China Knowledge Resource Integrated, and Web of Science. The quality of eligible studies was scored with the Quality Assessment of Diagnostic Accuracy Studies. The bivariate meta-analysis model was used to pool the sensitivity, specificity, likelihood ratio, and diagnostic odds ratio. Receiver operating characteristic curves and hierarchical summary receiver operating characteristic models were employed to check the overall test performance in this meta-analysis. Seven publications involving 678 patients and 563 controls were included in this meta-analysis. The pooled sensitivity was 0.84 (95% confidence interval: 0.80–0.88), specificity was 0.87 (95% confidence interval: 0.75–0.94), positive likelihood ratio was 6.5 (95% confidence interval: 3.10–13.62), negative likelihood ratio was 0.18 (95% confidence interval: 0.13–0.25), and diagnostic odds ratio was 36 (95% confidence interval: 13–99). The area under the summary receiver operating characteristic curve was 0.89 (95% confidence interval: 0.86–0.91). Our results indicated that urothelial carcinoma–associated 1 was a potential diagnostic biomarker with good specificity and sensitivity in urinary bladder cancer. Further prospective studies with larger cohorts are necessary to evaluate the diagnostic accuracy of urothelial carcinoma–associated 1 for urinary bladder cancer.
Targeting interlukin-6 to relieve immunosuppression in tumor microenvironment
Authors: Liu Qian , Yu Shengnan , Li Anping , Xu Hanxiao , Han Xinwei , Wu Kongming
Published: June 2017

Immunotolerance is one of the hallmarks of malignant tumors. Tumor cells escape from host immune surveillance through various mechanisms resulting in tumor progression and therapeutic resistance. Interlukin-6 is a proinflammatory cytokine involved in many physiological and pathological processes by integrating with multiple intracellular signaling pathways. Aberrant expression of interlukin-6 is associated with the growth, metastasis, and chemotherapeutic resistance in a wide range of cancers. Interlukin-6 exerts immunosuppressive capacity mostly by stimulating the infiltrations of myeloid-derived suppressor cells, tumor-associated neutrophils, and cancer stem-like cells via Janus-activated kinase/signal transducer and activator of transcription 3 pathway in tumor microenvironment. On this foundation, blockage of interlukin-6 signal may provide potential approaches to novel therapies. In this review, we introduced interlukin-6 pathways and summarized molecular mechanisms related to interlukin-6-induced immunosuppression of tumor cell. We also concluded recent clinical studies targeting interlukin-6 as an immune-based therapeutic intervention in patients with cancer.
The transformative potential of using participatory community sport initiatives to promote social cohesion in divided community contexts
Authors: Meir David , Fletcher Thomas
Published: June 2017

Sports are popularly believed to have positive integrative functions and are thought, therefore, to be able to galvanise different, and sometimes divided communities through a shared sporting interest. UK government and policy rhetoric over the last two decades has consistently emphasised the positive role sport can play in building more cohesive, empowered and active communities. These positive impacts are particularly important for communities with high numbers of young people from disadvantaged backgrounds. The purpose of this article is to reflect on the challenges associated with co-producing a participatory community sport initiative with 28 young people, the aim of which was to work towards greater social cohesion in an ethnically segregated borough in north-west England. Although a great deal was learned from working towards this, the initiative was ultimately unsuccessful because, for a variety of reasons, the young people removed themselves from the process. A major contribution of this article is its consideration of how we reflect on the realities of project failure and how future community sport initiatives might have greater success. In particular, we argue that for sport to make a difference, participants must be enabled to develop a sense of civic engagement and critical awareness which go beyond either sport or community development, emphasising wider sociopolitical development instead.
Marital Satisfaction, Conflict Resolution Styles, and Religious Attendance Among Latino Couples
Authors: Stinson Morgan A. , Bermúdez J. María , Gale Jerry , Lewis Denise , Meyer Andrea S. , Templeton G. Bowden
Published: June 2017

Research related to the process of communication among couples is central to the work of couple and family therapists. This study examines the relationship between couple’s conflict resolution styles, weekly church attendance, and marital satisfaction. Specifically, we surveyed 191 Latino couples using Gottman’s typology of conflict resolution styles (e.g., validator, avoidant, and volatile) to identify which style predicted marital satisfaction for both partners. Using the actor–partner interdependence model, we find a multifaceted picture of how partner’s conflict resolution style influences theirs and their partner’s marital satisfaction.
Assessing Narcissism Using Rorschach-Based Imagery and Behavior Validated by Clinician Reports: Studies With Adult Patients and Nonpatients
Authors: Gritti Emanuela S. , Marino David P. , Lang Margherita , Meyer Gregory J.
Published: June 2017

We evaluate 11 Rorschach variables with potential for assessing grandiosity and narcissism. Seven of these variables were drawn from previous literature: Omnipotence, Idealization, Reflection, Personal Knowledge Justification, Exhibitionism, Magic, and Elevated Mood States; four were developed for this research: Expanded Personal Reference, Narcissistic Devaluation, Narcissistic Deflation, and Narcissistic Denial. Using Rorschach protocols from American normative adults and Italian adult outpatients, the dimensional structure of these variables was evaluated by principal components analysis, and validity was tested by correlations with clinician ratings of narcissism on two scales from the Shedler–Westen Assessment Procedure–200 that were made after at least five sessions with the primary clinician. A cohesive dimension was found in both data sets defined by Expanded Personal Reference, Personal Knowledge Justification, Omnipotence, and Idealization, and it was meaningfully correlated with the clinician ratings of narcissism ( M r = .41). Implications of the findings include the applicability of these variables in clinical practice and research for assessing narcissistic personality dynamics.
Absorbing Customer Knowledge
Authors: Storey Chris , Larbig Christine
Published: June 2017

Customers are a knowledge resource outside of the firm that can be utilized for new service success by involving them in the design process. However, existing research on the impact of customer involvement (CI) is inconclusive. Knowledge about customers’ needs and on how best to serve these needs (articulated in the service concept) is best obtained from customers themselves. However, codesign runs the risk of losing control of the service concept. This research argues that of the processes of external knowledge, acquisition (via CI), customer knowledge assimilation, and concept transformation form a capability that enables the firm to exploit customer knowledge in the form of a successful new service. Data from a survey of 126 new service projects show that the impact of CI on new service success is fully mediated by customer knowledge assimilation (the deep understanding of customers’ latent needs) and concept transformation (the modification of the service concept due to customer insights). However, its impact is more nuanced. CI exhibits an “∩”-shaped relationship with transformation, indicating there is a limit to the beneficial effect of CI. Its relationship with assimilation is “U” shaped, suggesting a problem with cognitive inertia where initial learnings are ignored. Customer knowledge assimilation directly impacts success, while concept transformation only helps success in the presence of resource slack. An evolving new service design is only beneficial if the firm has the flexibility to adapt to change.
Onychomycosis in the 21st Century: An Update on Diagnosis, Epidemiology, and Treatment
Authors: Gupta Aditya K. , Versteeg Sarah G. , Shear Neil H.
Published: June 2017

Onychomycosis accounts for 50% of all nail disease cases and is commonly caused by dermatophytes. Diabetes, human immunodeficiency virus, immunosuppression, obesity, smoking, and advancing age are predisposing factors of this fungal infection. Potassium hydroxide and culture are considered the current standard for diagnosing onychomycosis, revealing both fungal viability and species identification. Other diagnostic tests currently available include periodic acid–Schiff staining, polymerase chain reaction techniques, and fluorescent staining. Across 6 recently published epidemiology studies, the global prevalence of onychomycosis was estimated to be 5.5%, falling within the range of previously reported estimates (2%-8%). Newly approved onychomycosis treatments include efinaconazole, tavaborole, and laser therapy with lasers only approved to temporarily increase the amount of clear nail. Additional onychomycosis treatments being investigated include iontophoresis and photodynamic therapy with small open-label studies reported thus far. Preventative strategies, to help decrease recurrence and reinfection rates, include sanitisation of footwear and prophylactic topical antifungal agents.
The destructive side of branding: A heuristic model for analyzing the value of branding practice
Authors: Bertilsson Jon , Rennstam Jens
Published: June 2017

Scholarship on branding has made important contributions in terms of the value-creating function of branding. However, previous literature has overemphasized the creation of value for organizations at the expense of an understanding of the destructive side of branding for organizations and society. Drawing on the work of Boltanski and Thevénot, we theorize value as different in separate ‘worlds of worth’ and offer a competing approach, arguing that the organizational practice of branding simultaneously and inevitably involves value-destructive aspects. In addition, to enable analysis of these value-destructive aspects, we argue that new understandings of what branding is are needed, and therefore, we introduce two new metaphors: branding as discursive closure and branding as hypocrisy. Based on these conceptual developments, the article offers a heuristic model for analyzing how, and what types of, value may be destroyed in organizational branding practice. We thereby contribute with a critical understanding of organizational branding that acknowledges the conflictual relationship between value regimes and enables a balanced analysis of the social consequences of branding.
Evaluation of employment-support services for adults with autism spectrum disorder
Authors: Nicholas David B , Zwaigenbaum Lonnie , Zwicker Jennifer , Clarke Margaret E , Lamsal Ramesh , Stoddart Kevin P , Carroll Cynthia , Muskat Barbara , Spoelstra Margaret , Lowe Katelyn
Published: June 2017

The employment rate among persons with autism spectrum disorder has been noted as unacceptably low. Employment-support services are increasingly linked to the potential for favorable job outcomes, yet little is known about employment-support practices and the outcome of these interventions. This mixed-methods study examined employment-support resources for persons with autism spectrum disorder. An online survey was completed by 137 senior clinicians or administrators in employment-support programs in Canada. Additionally, 122 follow-up interviews were conducted with individuals with autism spectrum disorder (n = 71) and their parents/caregivers (n = 51). Findings indicate that the quality and beneficial impact of employment-support services for adults with autism spectrum disorder may be more favorably perceived by employment-support personnel than by individuals with autism spectrum disorder and their families. Furthermore, employment-support personnel were more disparaging about autism spectrum disorder vocational support capacity within their community, compared to their own programs. Individuals with autism spectrum disorder and their families seek services that support both accessing and retaining employment. Capacity-building in employment support for youth and adults with autism spectrum disorder is recommended, based on a reported insufficiency of, and a lack of evidence guiding, existing services. Program recommendations and an emerging model for integrated vocational support in autism spectrum disorder are offered.
Desert island data: an investigation into researcher positionality
Authors: Dean Jon , Furness Penny , Verrier Diarmuid , Lennon Henry , Bennett Cinnamon , Spencer Stephen
Published: June 2017

The nature of qualitative research means that the personal values of an individual researcher can and do (unwittingly) shape the way in which they analyse data sets, and the resultant conclusions drawn. However this phenomenon is under-studied in social research and this article seeks to help rectify this. This article presents findings from a small research project focused on discourses of class, masculinity and work among British male comedians from working-class backgrounds, interviewed on the popular BBC Radio 4 radio programme Desert Island Discs. Six different researchers, from varying disciplinary, methodological and theoretical groundings, as well as from varying personal backgrounds, analysed three interview recordings and transcripts separately. All the researchers wrote up their individual analyses of these interviews and wrote reflexive pieces examining why they thought they approached the data as they did. The researchers then came together as a group to compare and contrast findings and approaches. The results from this study, including the discrepancies and distinctions and final group analysis, are reported alongside a thorough discussion of the project’s methodology. We find that the project evidenced how a diverse research team can bring out deeper and richer analyses, and was a refreshing way to try and answer questions of individual and collective positionality
Implications for equity and diversity of increasing international student numbers in European universities: Policies and practice in four national contexts
Authors: Haapakoski Jani , Pashby Karen
Published: June 2017

This paper examines the main rationales for and possible implications of the policy of increasing international student numbers in higher education (HE). Drawing on critical discourse analysis, we map key themes emerging from two sets of data—university strategy documents and interviews with staff—collected at eight universities in four national contexts in Europe as a part of a larger project focused on ethical internationalism in HE. In our analysis of the data, we apply social cartographic mapping to consider overlapping, competing and absent discourses related to the push to increase international student numbers by using a heuristic developed in the larger project. We found the imperative to increase international student numbers to be largely driven by economic rationales across different national contexts, reflective of a corporatization trend. Where more civic rationales are presented, these discourses are ultimately framed and mediated by neoliberalism. The findings contribute insight into the complicated discursive terrain of internationalising HE. The mapping makes visible what can be taken for granted or is left unexamined. It serves as a jumping-off point for reflection on the policy, practice and research of internationalisation in HE, promoting the formulation of key questions around the assumed benefits and ethics of internationalisation.
Family-centred services for young children with intellectual disabilities and their families: Theory, policy and practice
Authors: Ingólfsdóttir Jóna G , Egilson Snæfrídur Thóra , Traustadóttir Rannveig
Published: June 2017

This paper outlines the reported discrepancies between the aims of the welfare services in Iceland and the experiences of parents raising young children with intellectual disabilities. Prevailing views on disability and service delivery were also considered. A multi case study design was employed to reflect the situation in different parts of the country. Families of eight children with intellectual disabilities and professionals in three different municipalities formed the cases. The findings reveal a high convergence between the three cases with variations based on individual experiences rather than geographical location. Overall, parents praised the preschools but experienced support services often as fragmented and uncompromising. Particular components of the services were consistently regarded as hard to reach and not in accordance with the needs of the family. Cultural-historical activity theory is introduced as a beneficial framework for further study and system improvement.
Metabolic syndrome: pathophysiology, management, and modulation by natural compounds
Authors: Rochlani Yogita , Pothineni Naga Venkata , Kovelamudi Swathi , Mehta Jawahar L.
Published: June 2017

Metabolic syndrome (MetS) represents a cluster of metabolic abnormalities that include hypertension, central obesity, insulin resistance, and atherogenic dyslipidemia, and is strongly associated with an increased risk for developing diabetes and atherosclerotic and nonatherosclerotic cardiovascular disease (CVD). The pathogenesis of MetS involves both genetic and acquired factors that contribute to the final pathway of inflammation that leads to CVD. MetS has gained significant importance recently due to the exponential increase in obesity worldwide. Early diagnosis is important in order to employ lifestyle and risk factor modification. Here, we review the epidemiology and pathogenesis of MetS, the role of inflammation in MetS, and summarize existing natural therapies for MetS.
Triptorelin for the relief of lower urinary tract symptoms in men with advanced prostate cancer: results of a prospective, observational, grouped-analysis study
Authors: Gil Thierry , Aoun Fouad , Cabri Patrick , Perrot Valérie , van Velthoven Roland
Published: June 2017

Background:

Lower urinary tract symptoms (LUTSs) may develop in men with prostate cancer (PCa) and can impact quality of life (QoL). Gonadotropin-releasing hormone (GnRH) agonists as androgen deprivation therapy are standard treatment for PCa, however, data are limited on their effects on LUTSs. A grouped analysis of national observational, non-interventional studies initiated in clinical practice was performed to assess the effectiveness of triptorelin in reducing moderate or severe LUTSs, measured using the International Prostate Symptom Score (IPSS) in men with advanced or metastatic PCa.

Methods:

Men with PCa and LUTSs scheduled to receive triptorelin (3-month or 1-month extended release formulation) were recruited into prospective, non-interventional studies at centres in Algeria, Australia, Belgium, China, Hungary, Romania and South Korea. The primary effectiveness endpoint was the proportion of patients with moderate or severe LUTSs, assessed by IPSS, after 48 weeks. Secondary endpoints included: total IPSS, QoL due to urinary symptoms (IPSS Question 8) and prostate-specific antigen (PSA) levels at 24 and 48 weeks.

Results:

A total of 2701 patients were recruited; 1851 patients with moderate or severe LUTSs at baseline (IPSS > 7), received triptorelin and had follow-up IPSS (efficacy population). The proportion of patients with moderate or severe LUTSs was reduced to 67.2% from baseline at week 48, following a reduction to 75.9% at week 24: the overall time effect was significant ( p < 0.001). QoL due to urinary symptoms significantly improved from a mean score of 3.7 at baseline, to adjusted means of 2.5 and 2.1, at weeks 24 and 48, respectively ( p < 0.001 versus baseline). Mean PSA levels were reduced from 158.8 ng/ml at baseline to 11.5 and 16.0 ng/ml at weeks 24 and 48, respectively.

Conclusions:

Within the limitations of these observational studies, improvements in LUTSs and QoL observed after 24 weeks and maintained at 48 weeks indicate that triptorelin-induced effects improve LUTSs in patients with advanced PCa.
Affective Interventions for High-Ability Students From 1984-2015: A Review of Published Studies
Authors: Jen Enyi
Published: June 2017

A search of the literature, from 1984 to 2015, revealed that only 17 empirical studies of direct affective intervention with high-ability students exist in the field of gifted education. These selective 17 studies, with the justifiable data published alongside them, were analyzed. Five major findings included. First, there is a need for more empirical studies of the direct affective interventions in the field. Second, there is a call for more studies with similar research interests to examine the previous findings because almost no two studies have a similar research focus. Third, the contents of direct affective interventions need to be clearly defined so that the similar intervention could be replicated. Fourth, boys and girls preferred same-gender group and group leaders. Fifth, a variety of methods were used. It is a positive finding that researchers used a variety of methods to measure the outcomes of the direct affective interventions.
Local law repercussions on EU extradition law
Authors: Niblock Rebecca , Oehmichen Anna
Published: June 2017

The present article examines the developments of extradition law in Europe, with a special focus on case law in England & Wales and Germany. It explores the effects that the case law of the European Court of Human Rights and the Court of Justice of the European Union has had on extradition law within Europe, and how the tensions between mutual trust and fundamental rights protection in this area have been addressed by the two jurisdictions.
The emerging EU extradition Law. Petruhhin and beyond
Authors: Costa Miguel João
Published: June 2017

In a recent decision, the Court of Justice of the European Union held that Member States which apply a nationality exception in their extradition relations with third States are bound, under certain circumstances, to accord equal protection to EU citizens other than their own nationals. This article evaluates the nature, scope, impact and meaning of this ruling, and looks into two other extradition cases that are currently awaiting preliminary rulings. The analysis conducted leads the author to claim that these constitute ground-breaking developments which qualify as the advent of a new area within EU criminal law.
The ecocrime directive and its translation into legal practice
Authors: Perilongo Giovanni F. , Corn Emanuele
Published: June 2017

Directive 2008/99/EC, on the protection of the environment through criminal law, was approved after fierce dispute among European institutions. Its aim was to harmonise the definition of environmental offences in the EU and to ensure that they would be punished with ‘proportionate, effective and dissuasive’ criminal sanction. The authors, after having outlined the notion of environmental crime that the harmonisation instrument wishes to combat, critically assess its policy objectives and investigate the legal and practical outcomes of its implementation. In the final paragraph, they discuss the challenges faced by national authorities when trying to translate such supranational legislation into court practices.
Prolonged duration of apnea test during brain death examination in a case of intraparenchymal hemorrhage
Authors: Nattanmai Premkumar , Newey Christopher R , Singh Ishpreet , Premkumar Keerthivaas
Published: June 2017

Objective:

Apnea test is required as part of the brain death examination. The duration of the apnea test is variable but typically requires 8–10 min. Prolonged apnea tests have been reported in the setting of hypothermia. Here, we describe a case of prolonged duration of apnea test secondary to a phenomenon called cardiac ventilation.

Methods:

The patient presented in coma with brainstem areflexia after having an intracerebral hemorrhage resulting in subfalcine, central, uncal, and tonsillar herniations. Confounding variables were excluded. Brain death testing was performed, and she was found to have brainstem areflexia. Pre-requisites for apnea test were then met.

Results:

Apnea testing, however, was prolonged at 110 min. When reconnected to ventilator, it was noted that she had small (30–35 cc) tidal volumes at a rate of her heart rate without respiratory effort. Ancillary testing with four-vessel cerebral angiogram confirmed cerebral circulatory arrest.

Conclusions:

To our knowledge, this is the longest reported case of apnea testing during brain death testing. Variables known to cause a delay in the rise of carbon dioxide (PaCO2) levels were excluded. We suspect the hyperdynamic cardiac state caused cardiac ventilations resulting in slow increase in carbon dioxide levels.
Understanding state preferences with text as data: Introducing the UN General Debate corpus
Authors: Baturo Alexander , Dasandi Niheer , Mikhaylov Slava J.
Published: June 2017

Every year at the United Nations (UN), member states deliver statements during the General Debate (GD) discussing major issues in world politics. These speeches provide invaluable information on governments’ perspectives and preferences on a wide range of issues, but have largely been overlooked in the study of international politics. This paper introduces a new dataset consisting of over 7300 country statements from 1970–2014. We demonstrate how the UN GD corpus (UNGDC) can be used as a resource from which country positions on different policy dimensions can be derived using text analytic methods. The article provides applications of these estimates, demonstrating the contribution the UNGDC can make to the study of international politics.
Hormonal contraception and breast cancer, what more do we need to know?
Authors: Marsden Jo
Published: June 2017

Most women use hormonal contraception for more than 30 years and for many, this may involve exposure in their older reproductive years when baseline breast cancer risk rises steeply. Overall, the risk of breast cancer diagnosis with exposure to hormonal contraception is very small and outweighed by its contraceptive benefits but despite this, there are still outstanding questions for all methods used in clinical practice due to paucity of available evidence, lack of which should not be taken to imply safety. This is exemplified by the following assumptions: the progestogen-only pill and long-acting reversible contraceptives are ‘breast-safe’ options in peri-menopausal women, use of the levonorgestrel intrauterine system for the management of endometrial pathology in breast cancer survivors is less likely to promote disease recurrence and the benefit all hormonal contraceptive methods confer in reducing unplanned pregnancy in women at high familial risk outweigh the risk of breast cancer diagnosis. There is no data on risk with the concurrent prescription of hormone replacement therapy in women exhibiting climacteric symptoms who are still menstruating. Advice of GPs and Community Sexual & Reproductive Health specialists will inevitably be sought about some or all these issues and in the absence of conclusive evidence from clinical studies, caution should be applied and women counselled appropriately.
Mortality and health-related quality of life in patients surgically treated for spondylodiscitis
Authors: Dragsted Casper , Aagaard Theis , Ohrt-Nissen Søren , Gehrchen Martin , Dahl Benny
Published: June 2017

Purpose:

To assess mortality, disability, and health-related quality of life (HRQL) in patients surgically treated for spondylodiscitis.

Methods:

A retrospective longitudinal study was conducted on all patients surgically treated for spondylodiscitis over a 6-year period at a single tertiary spine center. Indications for surgery, pre- and postoperative neurological impairment, comorbidities, and mortality were recorded. A survey was conducted on all eligible patients with the EuroQol 5-dimension (EQ-5D) questionnaire and Oswestry Disability Index (ODI).

Results:

Sixty-five patients were diagnosed with spondylodiscitis not related to recent spine surgery. One-year mortality rate was 6%. In all, 36% and 27% had pre- and postoperative neurological impairment, respectively, with only one patient experiencing deterioration postoperatively. At final follow-up (median 2 years), mean ODI was 31% (SD = 22) and mean EQ-5D time trade-off score was 0.639 (SD = 0.262); this was significantly lower than that in the normal population ( p < 0.001). Patients with neurological impairment prior to index surgery had lower EQ-5D scores ( p = 0.005) and higher ODI ( p = 0.02) at final follow-up compared with patients without neurological impairment.

Conclusions:

Several years after surgery, patients surgically treated for spondylodiscitis have significantly lower HRQL and more disability than the background population. Neurological impairment prior to index surgery predicts adverse outcome in terms of disability and lower HRQL.