bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Synthesis of Silver-Incorporated Rutile TiO2 for Enhanced Photocatalytic Degradation of Ciprofloxacin and Carmine G Dye Pollutants
Authors: Abd El-Lateef Hany M. , Zeng Chao-Qun , Khalaf Mai M. , Mohamed Ibrahim M. A.
Published: December 2024

Developing sustainable TiO2-based photocatalysts for environmental remediation is an increasingly significant area of research. However, a limited understanding of the long-term ecological impact of these photocatalysts poses a barrier to their practical and industrial-scale applications. To address this challenge, this work employed a green synthesis approach to prepare an Ag/TiO2 photocatalyst designed to improve environmental compatibility and enhance efficiency in pollutant degradation. Ag/TiO2 was synthesized using mushroom biomass as a natural capping to evaluate its effectiveness in the degradation of ciprofloxacin (CIP) and azo Carmine G dye (ACGD). The mushroom biomass served as a renewable cost-effective support for Ag incorporation, while the Ag modification of TiO2 could enhance the photocatalyst’s performance. Structural, chemical, and morphological characterization techniques were applied and showed that the Ag/TiO2 particles consisted of irregularly shaped nanoparticles. The CIP removal reached 82.46% after 300 min and ACGD removal efficiency went up to 83.64%. The enhanced performance is attributed to the unique electronic and structural properties of Ag-modified TiO2. This study highlights the potential of Ag/TiO2 synthesized via green methods as a high-performance photocatalyst for the effective remediation of pharmaceutical and dye pollutants in wastewater treatment applications.
Efficient Synthesis of γ-Aminobutyric Acid from Monosodium Glutamate Using an Engineered Glutamate Decarboxylase Active at a Neutral pH
Authors: Miao Lulu , Zheng Yingjia , Cheng Runxi , Liu Jianzhong , Zheng Zhinan , Yang Hong , Zhao Jing
Published: December 2024

The efficient production of γ-aminobutyric acid (GABA) at a neutral pH remains a challenge due to the pH sensitivity of glutamate decarboxylase (GAD) enzymes. Our study addressed this limitation by identifying and engineering GAD enzymes with high activity under neutral conditions. Through gene mining, we discovered a wild-type GAD from Enterococcus faecalis (EfGAD) with high activity at pH 7.0 and, using zero-shot (ZS) predictor-guided mutagenesis and C-terminal truncation, we developed an EfGAD variant with a significantly enhanced catalytic efficiency. This variant demonstrated a 1.3-fold increase in GABA production (~300 g/L) from monosodium glutamate (MSG) compared to the wild-type EfGAD in 5 L bioreactor experiments. The ability to operate at a neutral pH without the need for acidic conditions reduces production costs and facilitates scalability. Our findings underscore the potential of integrating machine learning tools for enzyme optimization and provide a sustainable approach to GABA biosynthesis using MSG as a substrate.
Painful Diabetic Neuropathy: Sex-Specific Mechanisms and Differences from Animal Models to Clinical Outcomes
Authors: Merlin Emma , Salio Chiara , Ferrini Francesco
Published: December 2024

Diabetes is a chronic and progressive disease associated with high blood glucose levels. Several co-morbidities arise from diabetes, the most common and severe one is diabetic neuropathy whose symptoms also include pain hypersensitivity. Currently, there are no effective therapies to counteract painful diabetic neuropathy or slow down the progression of the disease, and the underlying mechanisms are yet to be fully understood. Emerging data in recent decades have provided compelling evidence that the molecular and cellular mechanisms underlying chronic pain are different across the sexes. Interestingly, relevant differences have also been observed in the course and clinical presentation of painful diabetic neuropathy in humans. Here, we reviewed the current state of the art on sex differences in diabetic neuropathy, from animal models to clinical data. Comparing the output of both preclinical and clinical studies is necessary for properly orienting future choices in pain research, refining animal models, and interpreting clinical data. The identification of sex-specific mechanisms may help to develop more targeted therapies to counteract pain symptoms in diabetes.
CycloZ Suppresses TLR4-Driven Inflammation to Reduce Asthma-Like Responses in HDM-Exposed Mouse Models
Authors: Lee Dohyun , Jeon Jongsu , Baek Seoyeong , Park Onyu , Kim Ah-Ram , Do Myoung-Sool , Jung Hoe-Yune
Published: December 2024

Asthma is a chronic lung disease characterized by airway inflammation, hyperresponsiveness, and narrowing, with a risk of life-threatening attacks. Most current treatments primarily consist of inhalable steroids, which are not without adverse effects. Recently, there has been growing interest in alternative approaches to asthma management. In this study, we investigated the anti-asthmatic effects of the non-steroidal compound CycloZ using acute and chronic mouse models of asthma. Allergic reactions were induced with house dust mite (HDM) extract, and CycloZ or fluticasone propionate (FP) was administered orally or intranasally, respectively. CycloZ significantly ameliorated the HDM-induced robust expression of Th2 cytokines in both models. CycloZ also decreased immune cell infiltration into the lungs and reduced IL-4 and IL-13 cytokine levels in bronchoalveolar lavage fluid (BALF). Moreover, CycloZ greatly attenuated the activation of the TLR-4 pathway, which is involved in HDM recognition and signaling. The beneficial effects of CycloZ were comparable to or even superior to the current steroid treatment, FP, suggesting that CycloZ could be a promising new option for asthma therapy.
Catalytic Reduction of the Compounds Generated When Heating Heet Tobacco in Presence of USY and Beta Zeolites and Silica Lovel 6000 and SBA-15 Silicate in Oxidative and Inert Atmospheres: Effect of Temperature and Catalyst Content
Authors: Marcilla Antonio , Berenguer Deseada , Beltrán María Isabel , Farcas Catalina
Published: December 2024

The thermal decomposition of a heat-not-burn (HNB) tobacco at four temperatures (250–400 °C) was studied via thermogravimetric analysis (TGA) and Multi-shot pyrolizer experiments (Py-GC/MS), and the effect of four potential additives, USY Beta and beta zeolites and Silica Lovel 6000 and SBA-15 silicates at three concentrations (5, 15 and 25% w/w) under an inert and oxidative atmosphere was analyzed. Different techniques were applied showing that the presence of the additives modifies the decomposition processes (TGA). Py-GC/MS showed that these tobaccos generate large amounts of Nicotine and Glycerine. Acid compounds are the most abundant compounds under an inert atmosphere, while Oxygenated compounds predominate under an oxidative atmosphere. In both atmospheres, Furans and Aromatics present in a significant abundance at high temperatures. The additives used reduce both the number and the concentration of most of the compounds generated, especially at high temperatures and concentrations. Moreover, SBA-15 shows good aptitudes to reduce the formation of some individual compounds included in the FDA’s HPHC list, such as Acetone and Acetaldehyde. Finally, smoking experiments corroborated that all additives produce marked reductions in TPM, i.e., the majority fraction obtained, and in practically all the compounds generated. Phenol, a toxicant compound that was detected in a significant amount, is also markedly reduced. SBA-15 is the material that presents a major reduction in the TPM and the principal compounds generated. These results may be of great interest for further reducing the toxicity of smoking this type of heat-not-burn tobacco product.
Photoacoustic Imaging of pH-Sensitive Optical Sensors in Biological Tissues
Authors: Kye Hyunjun , Jo Dongyoung , Jeong Sanghwa , Kim Chulhong , Kim Jeesu
Published: December 2024

Photoacoustic imaging is an emerging biomedical imaging technique that enables non-invasive visualization of the optical absorption properties of biological tissues in vivo. Although numerous studies have used contrast agents to achieve high-contrast imaging in deep tissues, targeting specific areas remains a challenge when using agents that are continuously activated. Recent research has focused on developing triggered contrast agents that are selectively activated in target areas. This review delves into the use of pH-triggered contrast agents in photoacoustic imaging, which take advantage of the lower pH of the tumor microenvironment compared to normal tissues. The paper discusses the mechanisms of pH-triggered contrast agents that contribute to improving depth and contrast in photoacoustic tumor imaging. In addition, the integration of functionalities, such as photothermal therapy and drug delivery monitoring, into these agents demonstrates significant potential for biomedical applications.
Parents’ Perception of the Role of Sports in Supporting Psychological and Social Development in Children with Specific Learning Disorders
Authors: Borges-Silva Fernanda , Manzanares Aarón , González-Gálvez Noelia , Zanzurino Giuseppe , Cordovani Gabriele , Cherubini Domenico , Morales-Belando María T.
Published: December 2024

Background: This study aims to examine parents’ perceptions of how coach support influences the satisfaction of basic psychological needs (autonomy, competence, and relatedness) and its subsequent impact on the self-esteem and overall well-being of children with Specific Learning Disorders (SLDs) through participation in sports. Methods: The sample consisted of 1146 parents of children and young people diagnosed with SLDs from several European countries. The Coach Support Scale (COS), the Basic Psychological Needs Satisfaction Scale (BPNS), the Rosenberg Self-Esteem Scale (RSES), and the Sport Impact Scale (SIS) were used. Descriptive, reliability, Gaussian distribution, and ANOVA analyses were conducted. Results: The results show that increased sports participation is associated with higher perceptions of coach support, autonomy, competence, psychological need satisfaction, self-esteem, relatedness, and the positive impact of sports on the lives of children and adolescents with SLDs. Children and teenagers who engage in individual and team sports exhibit greater coach support, autonomy, competence, relatedness, BPNS, and self-esteem compared to those who participate in only one type of sport, with p-values ranging from 0.004 to 0.050. Conclusions: In conclusion, participation in sports seems to benefit the self-esteem and well-being of children and young people with SLDs. The practical applications of this study highlight the importance of specialized coach training to address the psychological needs of children with SLDs, the development of balanced sports programs that integrate individual and team activities to optimize benefits, the encouragement of parental involvement to enhance positive experiences, and the implementation of policies that support inclusive and adaptive sports practices.
The Impact of the COVID-19 Pandemic on the Epidemiology and Management Strategies of Forearm Fractures in Children—a Retrospective Study
Authors: David Vlad Laurentiu , Garjoaba Cristina Ana-Maria , Popescu-Rohlicek Diana , Szentpeteri Larisa Anca , Ciornei Bogdan , Popoiu Calin Marius , Boia Eugen Sorin
Published: December 2024

Background: The COVID-19 pandemic has had a considerable influence over the management strategies in pediatric trauma all over the world. We are making a comparative assessment of all pediatric forearm fracture presentations in a tertiary center in Romania in a pre-pandemic year 2019 (NPG) versus a pandemic year 2021 (PG). Material and Methods: We retrospectively compared the epidemiological, the anatomopathological, and the management features of forearm fractures for the two years. Results: A total of 1403 patients with forearm fractures, 720 in NPG and 683 in PG, ages < 1 year–19 years (mean for NPG = 9.38 years and mean for PG = 9.39 years), were included in the study. There are no differences in demographics of the patients. There was an increase in the angulation ratio in the PG group and no other differences in the anatomopathological features. Most of the patients were treated by non-surgical means with no differences between groups. In PG, titan elastic nails were more often used than K-wire for the stabilization of diaphyseal fractures. There was no difference in terms of complication rates and reinterventions between groups. The number of hospital admissions (p < 0.01) and the length of hospital stay were significantly higher in NPG versus PG, (p < 0.01). Conclusions: The COVID-19 pandemic has had little impact on the epidemiology, anatomopathological features, and management strategies of forearm fractures in children. The only significant change in the medical strategy in our series was towards reducing the hospitalization rate and duration, reducing the follow-up visits.
Rhabdomyosarcoma of the Middle Ear Case Report
Authors: Markov Stoyan Stefanov , Spasova Mariya Ivanova , Spasov Neofit Iuriev , Markova Petya Petkova
Published: December 2024

Background: Rhabdomyosarcoma (RMS) is a highly malignant soft tissue tumor derived from primitive embryonal mesenchymal tissue that differentiates into striated skeletal muscle. Despite the improved outcome based on the EFS and OS using the three different treatment modalities-chemotherapy, radiotherapy and surgical treatment, the survival of patients depends on their IRS groups—pathological and surgical. On the other hand in the last thirty years a great improvement of the five-year overall survival (OS) of children with RMS have been observed based on the results of large multinational collaborative trials and successive studies dedicated to children, though prognosis is variable and dependent on several factors including histologic variant, primary sites of the tumor, extent of disease (disease resectability), and molecular-level characteristics. Case presentation: We present a clinical case of a five-year-old male with initial complains of left side peripheral facial nerve palsy and secondary cervical and retroauricular lymphadenomegaly. After an exam, surgery of the temporal bone, CT and MRI embryonal type of rhabdomyosarcoma was diagnosed, and adjuvant chemotherapy was initiated in combination with concomitant local radiotherapy. Results: The results show that in these areas surgery itself is insufficient for RMS treatment(usually it is limited to taking a biopsy only). The combination of chemotherapy and local control with radiotherapy achieved a good result in our patient. Conclusions: Middle ear Embryonal Rhabdomyosarcoma is a common solid tumor, which could mimic middle ear inflammation or mastoid inflammation in patients. The multimodal approach seemed to be the ideal management of RMS. It involves a combination of chemotherapy and local control with surgery and/or radiotherapy.
Be Divergent, Be Green! The Moderating Role of Gender in the Association Between Divergent Thinking and Pro-Environmental Behaviours in Children
Authors: Sannino Marta , Galli Elisa , Zacheo Cristina , Giancola Marco
Published: December 2024

Background/Objectives: The environmental crisis has begun as a daily challenge for present and forthcoming generations. This scenario highlights the need to adopt many pro-environmental strategies to avoid its adverse consequences. Consequently, it is of paramount importance to comprehend the fundamental psychological and cognitive characteristics that may encourage young children to participate in Pro-Environmental Behaviours (PEBs). Previous research has explored key psychological factors like values, norms, and beliefs influencing children’s pro-environmental behaviours (PEBs), but the impact of cognitive processes is still debated. This research explored the association between divergent thinking (DT) and PEBs, also addressing the potential involvement of gender. Methods: The study involved 348 children (Mage = 8.78 years; SDage = 1.79; range age 6–13; 174 girls) who completed a socio-demographic questionnaire, the Pro-Environmental Behaviour Questionnaire, and the Alternative Uses Task (AUT). Results: The statistical analysis indicated that gender moderates the relationship between children’s DT-creativity and PEBs (B = 0.08, SE = 0.04, t = 2.05; 95% CIs [0.0033, 0.1659]), strengthening this association. Conclusions: These findings yielded further evidence on the impact of cognitive processes, such as DT on PEBs, extending the knowledge regarding the critical role of gender in this intricate relationship.
The Relationship Between Manual Dexterity and Toothbrushing Efficiency in Preschool Children: A Crossover Study
Authors: Macote-Orosco Lisbeth , Martín-Vacas Andrea , Paz-Cortés Marta M. , Mourelle Martínez María Rosa , de Nova M. Joaquín
Published: December 2024

Aim: The aim of this study was to evaluate the relationship between oral hygiene (OH) efficiency and manual dexterity skills in Spanish five-year-old children using two fine motor tests. Methods: A cross-sectional study with a pre–post evaluation was designed. The children’s OH was measured according to the Silness and Löe plaque index (PI) before toothbrushing upon arrival at school (pre) and after supervised toothbrushing (post). Motor skills (children’s dexterity) were determined with the Visual Motor Skills and Resistance to Fatigue test (VMSRF test) and the scale for the Behavioural Assessment of Preschool Skills (BAPS scale). Data were analysed with the SPSS® statistics software with a 95% confidence interval and bilateral significance. Results: One hundred and twenty-nine children were evaluated. The mean PI was 2.5, indicating generally poor OH in the sample. No difference in OH was observed between genders or in the brushing frequency categories. No significant differences in OH or the PI were found between right- and left-handed children either (p > 0.05). Significant differences were found between the OH category pre and post toothbrushing in the total sample, as well as in the various sextants and surfaces evaluated. The results of the VMSRF and BAPS tests indicate moderate visuomotor skills and fatigue resistance and a poor average skill level. A statistically significant improvement in the PI was found in children with higher scores in the VMSRF and BAPS tests. Conclusions: A significantly higher OH efficacy was found in children with better motor skills, although five-year-old children showed moderate visuomotor skills and fatigue resistance and a poor average preschool skill level.
The Impact of Dog-Assisted Therapy Among Children and Adolescents with Autism Spectrum Disorder: A Systematic Review
Authors: Galvany-López Paula , Martí-Vilar Manuel , Hidalgo-Fuentes Sergio , Cabedo-Peris Javier
Published: December 2024

Background and Objectives: Animal-assisted therapies have been utilized in various profiles to improve people’s quality of life. This systematic review aims to assess the impact of dog-assisted therapies (DAT) on children and adolescents with Autism Spectrum Disorder (ASD). The benefits provided, the feasibility of implementation, and potential limitations are analysed. Methods: An exhaustive search was carried out in the following databases: Web of Science (WoS), Scopus, ERIC, PubMed, PubPsych, CINAHL, PsycInfo and Dialnet. The procedure was preregistered on PROSPERO. Results: This review included 19 scientific articles. Of these, 84% suggest that DAT has a positive impact on communication and social interaction during the development of children and adolescents diagnosed with ASD. The dog is often perceived as a communication facilitator that serves as a reinforcer, capturing the individual’s attention. Emotional benefits have also been reported, including reducing stereotyped and self-harming behaviours. Additionally, an increase in smiling frequency and self-esteem levels was observed. Conclusions: These findings highlight the potential of DAT as a component of socio-educational intervention for children and adolescents diagnosed with ASD. However, further research is necessary due to the limited sample sizes in the studies reviewed.
The Role of Psychological Capital on Climate Change Adaptation Among Smallholder Farmers in the uMkhanyakude District of KwaZulu-Natal, South Africa
Authors: Maziya Mbongeni , Mdoda Lelethu , Mvelase Lungile Pearl Sindiswa
Published: December 2024

Climate change and variability pose a challenge to the livelihoods of smallholder farmers. Previous studies on climate change in the context of smallholder farming have mainly focused on the influence of socio-economic factors in understanding farmers’ responses to climate change. However, little is known about the effect of psychological capital on climate change adaptation. There are calls for better empirical models and transdisciplinary approaches to understand the underlying drivers of climate change adaptation in smallholder farming systems. This study draws from behavioural decision research to assess psychological factors influencing climate change adaptation in the uMkhanyakude district of KwaZulu-Natal. This study adopted the Theory of Planned Behaviour to understand the effect of psychological capital on climate change adaptation. Data were collected from a sample of 400 smallholder farmers who were randomly selected from the uMkhanyakude district. Survey data were analysed using a multivariate probit regression model. The results of the multivariate probit regression model indicated that psychological capital (attitudes towards climate change, subjective norms, and trust) played an important role in influencing climate change adaptation. Climate change adaptation is also influenced by the gender of the farmer, education level, household size, and Tropical Livestock Units. These findings underscore the role of psychological capital in shaping climate change adaptation. This study recommends using transdisciplinary approaches (i.e., combining economics and psychology) in evaluating farmers’ responses to climate change.
Editorial “Blockchain Technology—A Breakthrough Innovation for Modern Industries”
Authors: Adamashvili Nino , Tricase Caterina , Zumburidze Otar , State Radu , Tonelli Roberto
Published: December 2024

In June 2022, the Italian national project PRIN (Research Projects of National Relevance), W [...]
1,2-Dihydroxy-9H-Xanthen-9-One, a Multifunctional Nature-Inspired Active Ingredient
Authors: Jesus Ana , Vieira Sara F. , Brites Gonçalo , Carrascal Mylène , Ferreira Helena , Neves Nuno M. , Cidade Honorina , Pinto Madalena , Sousa Emília , Almeida Isabel F. , Cruz Maria T.
Published: December 2024

Incorporating antioxidants into cosmetics is the mainstay for developing new products to mitigate skin aging. However, identifying novel multifunctional antioxidant ingredients with additional relevant properties that block the skin hallmarks of aging is a very striking strategy. Many natural compounds, including xanthones, have demonstrated biologically notable properties. In particular, 1,2-dihydroxy-9H-xanthen-9-one (1,2-DHX) has inhibitory activity against skin enzymes, and metal-chelating and radical-scavenging activities. Therefore, 1,2-DHX is an attractive molecule for cosmetic purposes. With this goal in mind, the anti-inflammatory, antioxidant, and anti-allergic potentials of 1,2-DHX were investigated. 1,2-DHX demonstrated anti-inflammatory properties by inhibiting the synthesis of specific pro-inflammatory mediators, including interleukin-6 (IL-6) and prostaglandin E2 (PGE2), in human macrophages. This xanthone did not elicit sensitization reactions and did inhibit allergic reactions triggered by a strong skin allergen, suggesting its potential as an anti-allergic compound. 1,2-DHX also revealed mitochondrial antioxidant activity by mitigating rotenone-induced oxidative stress in macrophages by up to 40%. Overall, 1,2-DHX displayed a safety profile and noteworthy biological activities, highlighting its multifunctional profile as an active cosmetic ingredient with anti-inflammatory, antioxidant, and anti-allergic properties.
Effects of Photopatterning Conditions on Azimuthal Surface Anchoring Strength
Authors: Haputhanthrige Nilanthi P. , Rajabi Mojtaba , Lavrentovich Oleg D.
Published: December 2024

Spatially varying alignment of liquid crystals is essential for research and applications. One widely used method is based on the photopatterning of thin layers of azo-dye molecules, such as Brilliant Yellow (BY), that serve as an aligning substrate for a liquid crystal. In this study, we examine how photopatterning conditions, such as BY layer thickness (b), light intensity (I), irradiation dose, and age affect the alignment quality and the strength of the azimuthal surface anchoring. The azimuthal surface anchoring coefficient, W, is determined by analyzing the splitting of integer disclinations into half-integer disclinations at prepatterned substrates. The strongest anchoring is achieved for b in the range of 5–8 nm. W increases with the dose, and within the same dose, W increases with I. Aging of a non-irradiated BY coating above 15 days reduces W. Our study also demonstrates that sealed photopatterned cells filled with a conventional nematic preserve their alignment quality for up to four weeks, after which time W decreases. This work suggests the optimization pathways for photoalignment of nematic liquid crystals.
Research on the Elastic Properties of Deep Rock Composites Under High Pressure
Authors: Li Linmei , Zeng Xianren , Tan Yonghong , Xu Qiyuan , Liu Shihuan
Published: December 2024

This paper presents a three-dimensional model of the definitive structure of gypsum, calcite and quartz crystals, which are the primary components of deep rock mass. The optimization of the primary components of deep rock mass material was performed under pressures ranging from 0 to 120 MPa using the first principles method. This led to the determination of the structural constants of the primary component crystals within the deep rock material. Furthermore, the variation rule of mechanical parameters, including elastic constants, for the primary components of deep-seated rock materials under different pressures was also investigated. The findings indicate a clear downward trend in the partial crystal structure parameters of deep-seated rock materials with increasing pressure. The primary components of each rock mass material display disparate compressibility properties in distinct directions when subjected to uniaxial compression. In conclusion, the anisotropic elastic properties of gypsum crystals, calcite crystals and quartz crystals under different pressures were investigated. The anisotropic cloud diagrams of bulk modulus, elastic modulus and shear modulus were presented for the three types of rock material at 0 MPa, 60 MPa and 120 MPa, respectively. In conclusion, we derived the theoretical equations for the elastic tensor matrix of composite materials using the principle of superposition. Furthermore, we studied and obtained the elastic tensor matrix for composite materials within the deep rock mass. These results provide essential theoretical guidance for the development of underground space and the analysis of deep rock dynamics.
Evaluations of Microstructure in Ultra-Fine-Grained Matrix with Sintering-Assisted ECAP Process in Aluminum–Nickel Powders
Authors: Güral Ahmet , Demir Ümit
Published: December 2024

The aim of this study was the synthesis of intermetallic crystal dispersion in an ultra-fine-grained (UFG) aluminum matrix by the equal-channel angular pressing (ECAP) process, which provides severe plastic deformation (SPD) of aluminum-5 and 10% wt. nickel powders. The ECAP process of up to 20 passes was carried out at 200 °C. Intermetallic crystal particles in the UFG Al matrix were obtained in sintered samples at 500 °C for 1 and 5 h, interrupting the ECAP process. The scanning electron microscope (SEM) images were received for the microstructural evaluations. According to the SEM images, it was understood according to the quantitative observation and energy dispersive spectroscopy (EDS) analysis results that the Ni powders added to Al mostly remained in the block state in the matrix structure after the ECAP processes but started to dissolve in the matrix by increasing the ECAP pass number and sintering temperature. DSC and XRD analyses were carried out to investigate intermetallic crystal evaluation in the material. According to DSC analyses, the melting degrees of the alloys increase with the amount of Ni added. Melting enthalpies and melting degrees showed small changes in the number of ECAP passes. Also, electron backscatter diffraction (EBSD) images were obtained for the samples’ grain size and grain boundary angle measurements. It has been understood that the number of passes and the process temperature are effective parameters for the solid-state synthesis of Al3Ni intermetallic crystals in UFG by the ECAP process.
Crystal Structures of Sulfobetaine-8 Solvates: Bend Hydrophobic Chains and Doubly Charge-Assisted Hydrogen Bonds N+CH⋯−O3S
Authors: Churakov Andrei V. , Anokhin Denis V. , Kalle Paulina , Kiseleva Marina A. , Vener Mikhail V. , Kuz’mina Lyudmila G.
Published: December 2024

Three novel solvatomorphs (C13H29NO3S•CH3OH, 1; C13H29NO3S•0.113(H2O), 2; C13H29NO3S•0.038(H2O), 3) of zwitterionic sulfobetaine-8 were obtained and their structures were determined using single-crystal X-Ray diffraction. In all cases dimethyl–amino substituted hydrophobic chains -(CH2)3-N+Me2-(CH2)7-Me exhibit kinks at nitrogen atoms resulted from strong intra- and intermolecular CH⋯O hydrogen bonds between negatively charged sulfonic anion -O3S- and positively charged tetraalkylammonium fragments. Periodic (solid state) DFT calculations for structure 1 showed that the energy of the intermolecular hydrogen bonds CH…O is very high, at about 17 kJ/mol. In hydrates 2 and 3, water molecules play the structure-forming role since they interconnect hydrophobic layers by HOH…-O3S hydrogen bonds. The location of only partially occupied water molecules in the interlayer space leads to low stability of both crystals 2 and 3 in open air.
Phylogeny and Genetic Population Structure of Dominant Copepods in Two Ponds with Contrasting Salinities in the Solar Saltern of Sfax (Tunisia) Based on Mitochondrial (COI and Cytb) and Nuclear (18S) DNA Sequences
Authors: Ladhar Chiraz , Denis Françoise , Guermazi Wassim , Annabi-Trabelsi Neila , Casse Nathalie , Ayadi Habib , Hotos George N.
Published: December 2024

Due to the complexity of taxonomic classification based on the classical morphological characters of copepods, phylogenies have been ambiguous. In this study, we investigate the phylogeny of copepods, including four species from three orders, in the saltern of Sfax using the small subunit of nuclear ribosomal RNA genes (18S). In the studied area, copepods seemed to be a polyphyletic group, and the genetic structure of these crustaceans is complex and problematic. We have also used two mitochondrial markers, the cytochrome c oxidase subunit I (mtCOI) gene and the cytochrome b (Cytb) sequence data, in order to investigate the genetic diversity and differentiation in a total of 96 individuals from two sets of Paracartia grani, sampled from two ponds with different salinities (42 PSU and 61 PSU). All of the results presented here suggest a low genetic diversity among P. grani species and a weak genetic structure between the sets. The nucleotide and haplotype diversity of P. grani were extremely low, indicating the homogeneity of the two sets, which could be combined into one set living in different ranges of salinity. This small genetic diversity is possibly due to the confined natural distribution range and strong selective pressure in a saltern environment. These data also suggest that gene flow is the main factor shaping the genetic structure of the studied sets.
Formalization for Subsequent Computer Processing of Kara Sea Coastline Data
Authors: Bogatova Daria , Ogorodov Stanislav
Published: December 2024

This study aimed to develop a methodological framework for predicting shoreline dynamics using machine learning techniques, focusing on analyzing generalized data without distinguishing areas with higher or lower retreat rates. Three sites along the southwestern Kara Sea coast were selected for this investigation. The study analyzed key coastal features, including lithology, permafrost, and geomorphology, using a combination of field studies and remote sensing data. Essential datasets were compiled and formatted for computer-based analysis. These datasets included information on permafrost and the geomorphological characteristics of the coastal zone, climatic factors influencing the shoreline, and measurements of bluff top positions and retreat rates over defined time periods. The positions of the bluff tops were determined through a combination of imagery with varying resolutions and field measurements. A novel aspect of the study involved employing geostatistical methods to analyze erosion rates, providing new insights into the shoreline dynamics. The data analysis allowed us to identify coastal areas experiencing the most significant changes. By continually refining neural network models with these datasets, we can improve our understanding of the complex interactions between natural factors and shoreline evolution, ultimately aiding in developing effective coastal management strategies.
Digital Papillary Adenocarcinoma Is HPV-42-Associated and BRAFV600E Negative: Perspectives for Diagnostic Practice
Authors: Dege Tassilo , Rütten Arno , Goebeler Matthias , Kneitz Hermann
Published: December 2024

Digital papillary adenocarcinoma (DPAC) is a rare, low-grade sweat gland carcinoma primarily found on the hands, fingers, or toes and predominantly affecting males. Distinguishing DPAC from benign sweat gland tumors can be challenging. We present the case of a 52-year-old patient with a progressive tumor on the finger initially misdiagnosed as a viral wart. Histological examination revealed a cytologically basophilic sweat gland tumor with tubular structures, papillary protrusions, and a characteristic immunohistochemical staining pattern for CK 7 and Actin. HPV-42 positivity and molecular analysis confirmed the diagnosis of DPAC. HPV-42 has been strongly associated with DPAC. Additionally, p16 positivity and BRAFV600E negativity were observed. These findings aid in the differential diagnosis of acral sweat gland tumors and guide clinical management, including with respect to the potential for recurrence and metastasis.
Lipoprotein(a) as a Risk Factor for Recurrent Acute Myocardial Infarction and Mortality: Insights from Routine Clinical Practice
Authors: Šuran David , Kanič Vojko , Kokol Peter , Završnik Tadej , Verhnjak Florjan , Žlahtič Bojan , Sinkovič Andreja , Naji Franjo Husam
Published: December 2024

Background: Lipoprotein(a) [Lp(a)] is a well-established risk factor for incident atherosclerotic cardiovascular (CV) disease. However, evidence regarding its association with recurrent events is limited. To address this gap, we conducted a retrospective analysis of routine clinical data, focusing on patients hospitalized for acute myocardial infarction (AMI) between 2000 and 2022 with available admission Lp(a) results. Methods: Patients were stratified into three groups based on their Lp(a) level (≤50 mg/dL, 51–90 mg/dL, and >90 mg/dL). A multivariable-adjusted Cox regression analysis was performed to assess the associations of Lp(a) with recurrent AMI, CV mortality, and all-cause mortality. Results: A total of 2248 patients (31.5% women), with a mean age of 64.7 ± 12.2 years, were retrospectively followed until 31 December 2022, or death. The multivariable-adjusted hazard ratios (HRs) for recurrent AMI were 1.01 (p = 0.921) for levels 51–90 mg/dL and 1.51 (p = 0.013) for levels > 90 mg/dL, compared with levels ≤ 50 mg/dL. The corresponding HRs for CV mortality were 1.13 (p = 0.300) and 1.14 (p = 0.348), and those for all-cause mortality were 1.09 (p = 0.310) and 1.20 (p = 0.090), respectively. Stratification by sex and age revealed a significant association of Lp(a) with recurrent AMI only in women aged > 65 years, with adjusted HRs of 2.34 (p = 0.013) for levels 51–90 mg/dL and 3.94 (p < 0.001) for levels > 90 mg/dL, compared with levels ≤ 50 mg/dL. Conclusions: In the presented study, Lp(a) was associated with a significantly higher risk of recurrent AMI only in women aged > 65 years with Lp(a) levels > 50 mg/dL. We found no significant associations between Lp(a) and CV or all-cause mortality.
Cardiac Magnetic Resonance Imaging with Myocardial Strain Assessment Correlates with Cardiopulmonary Exercise Testing in Patients with Pectus Excavatum
Authors: Lollert André , Abu-Tair Tariq , Emrich Tilman , Kreitner Karl-Friedrich , Sterlin Alexander , Kampmann Christoph , Staatz Gundula
Published: December 2024

Objectives: To evaluate correlations between cardiac magnetic resonance imaging (cMRI) at rest including strain imaging and variables derived from quantitative cardiopulmonary exercise testing using a treadmill in patients with pectus excavatum. Methods: We retrospectively correlated the results of cMRI and cardiopulmonary exercise testing in 17 patients with pectus excavatum, in whom both examinations were performed during their pre-operative clinical evaluation. In addition to cardiac volumetry, we assessed the strain rates of both ventricles using a feature-tracking algorithm of a piece of commercially available post-processing software. Results: Right ventricular (RV) ejection fraction correlated negatively with heart rate at anaerobic threshold (rho = −0.543, p = 0.024). A positive correlation between radial strain rate at the RV base and percentage of predicted maximum heart rate (rho = 0.72, p = 0.001) was shown, with equivalent results for circumferential strain rate (rho = −0.64, p = 0.005). Radial strain rate at the RV base correlated in a strongly negative way with maximum oxygen uptake (rho = −0.8, p < 0.001), with a correspondingly positive correlation for circumferential strain rate (rho = 0.73, p = 0.001). Conclusions: Quantitative parameters derived from cMRI at rest, especially those acquired at the most severely compressed RV base, correlated with cardiopulmonary exercise testing variables. The compression of the RV base by the sternum might be partially compensated by an increased strain rate to induce higher heart frequencies during exercise. However, high strain rates were associated with a higher disease severity and a lower maximum oxygen uptake, indicating a limitation of this compensation mechanism.
Applying Neural Networks to Analyse Inflammatory, Sociodemographic, and Psychological Factors in Non-Melanoma Skin Cancer and Colon Cancer: A Statistical and Artificial Intelligence Approach
Authors: Mercuţ Răzvan , Ciurea Marius Eugen , Traşcă Emil Tiberius , Ionescu Mihaela , Mercuţ Maria Filoftea , Rădulescu Patricia Mihaela , Călăraşu Cristina , Streba Liliana , Ionescu Alin Gabriel , Rădulescu Dumitru
Published: December 2024

Background/Objectives: Chronic inflammation and psychosocial factors significantly influence cancer progression and patient behavior in seeking medical care. Understanding their interplay is essential for enhancing early detection and developing personalized treatment strategies. This study aims to develop a comprehensive patient profiling model by comparing non-melanoma skin cancer (NMSC) and colorectal cancer (CRC). The goal is to identify common and distinct patterns in inflammation and psychosocial factors that affect disease progression and clinical presentation. Methods: We conducted a comparative analysis of patients diagnosed with NMSC and CRC, integrating clinical data with sociodemographic and psychological assessments. Advanced neural network algorithms were employed to detect subtle patterns and interactions among these factors. Based on the analysis, a cancer risk assessment questionnaire was developed to stratify patients into low-, moderate-, and high-risk categories. Results: Patients with low systemic inflammation and adequate vagal tone, supported by a stable family environment, demonstrated heightened sensitivity to subclinical symptoms, enabling earlier diagnosis and timely intervention. Conversely, patients with high systemic inflammation and reduced vagal tone, often influenced by chronic stress and unstable family environments, presented at more advanced disease stages. The developed risk assessment tool effectively classified patients into distinct risk categories, facilitating targeted preventive measures and personalized therapeutic strategies. Neural network profiling revealed significant interactions between biological and psychosocial factors, enhancing our understanding of their combined impact on cancer progression. Conclusions: The integrated profiling approach and the newly developed risk assessment questionnaire have the potential to transform cancer management by improving early detection, personalizing treatment strategies, and addressing psychosocial factors. This model not only enhances clinical outcomes and patient quality of life but also offers a framework adaptable to other cancer types, promoting a holistic and patient-centered approach in oncology.
Assessing Patient Health Dynamics by Comparative CT Analysis: An Automatic Approach to Organ and Body Feature Evaluation
Authors: Müller Dominik , Voran Jakob Christoph , Macedo Mário , Hartmann Dennis , Lind Charlotte , Frank Derk , Schreiweis Björn , Kramer Frank , Ulrich Hannes
Published: December 2024

Background/Objectives: The integration of machine learning into the domain of radiomics has revolutionized the approach to personalized medicine, particularly in oncology. Our research presents RadTA (RADiomics Trend Analysis), a novel framework developed to facilitate the automatic analysis of quantitative imaging biomarkers (QIBs) from time-series CT volumes. Methods: RadTA is designed to bridge a technical gap for medical experts and enable sophisticated radiomic analyses without deep learning expertise. The core of RadTA includes an automated command line interface, streamlined image segmentation, comprehensive feature extraction, and robust evaluation mechanisms. RadTA utilizes advanced segmentation models, specifically TotalSegmentator and Body Composition Analysis (BCA), to accurately delineate anatomical structures from CT scans. These models enable the extraction of a wide variety of radiomic features, which are subsequently processed and compared to assess health dynamics across timely corresponding CT series. Results: The effectiveness of RadTA was tested using the HNSCC-3DCT-RT dataset, which includes CT scans from oncological patients undergoing radiation therapy. The results demonstrate significant changes in tissue composition and provide insights into the physical effects of the treatment. Conclusions: RadTA demonstrates a step of clinical adoption in the field of radiomics, offering a user-friendly, robust, and effective tool for the analysis of patient health dynamics. It can potentially also be used for other medical specialties.
Validation of Artificial Intelligence Computer-Aided Detection of Colonic Neoplasm in Colonoscopy
Authors: Lee Hannah , Chung Jun-Won , Kim Kyoung Oh , Kwon Kwang An , Kim Jung Ho , Yun Sung-Cheol , Jung Sung Woo , Sheeraz Ahmad , Yoon Yeong Jun , Kim Ji Hee , Kayasseh Mohd Azzam
Published: December 2024

Background/Objectives: Controlling colonoscopic quality is important in the detection of colon polyps during colonoscopy as it reduces the overall long-term colorectal cancer risk. Artificial intelligence has recently been introduced in various medical fields. In this study, we aimed to validate a previously developed artificial intelligence (AI) computer-aided detection (CADe) algorithm called ALPHAON® and compare outcomes with previous studies that showed that AI outperformed and assisted endoscopists of diverse levels of expertise in detecting colon polyps. Methods: We used the retrospective data of 500 still images, including 100 polyp images and 400 healthy colon images. In addition, we validated the CADe algorithm and compared its diagnostic performance with that of two expert endoscopists and six trainees from Gachon University Gil Medical Center. After a washing-out period of over 2 weeks, endoscopists performed polyp detection on the same dataset with the assistance of ALPHAON®. Results: The CADe algorithm presented a high capability in detecting colon polyps, with an accuracy of 0.97 (95% CI: 0.96 to 0.99), sensitivity of 0.91 (95% CI: 0.85 to 0.97), specificity of 0.99 (95% CI: 0.97 to 0.99), and AUC of 0.967. When evaluating and comparing the polyp detection ability of ALPHAON® with that of endoscopists with different levels of expertise (regarding years of endoscopic experience), it was found that ALPHAON® outperformed the experts in accuracy (0.97, 95% CI: 0.96 to 0.99), sensitivity (0.91, 95% CI: 0.85 to 0.97), and specificity (0.99, 95% CI: 0.97 to 0.99). After a washing-out period of over 2 weeks, the overall capability significantly improved for both experts and trainees with the assistance of ALPHAON®. Conclusions: The high performance of the CADe algorithm system in colon polyp detection during colonoscopy was verified. The sensitivity of ALPHAON® led to it outperforming the experts, and it demonstrated the ability to enhance the polyp detection ability of both experts and trainees, which suggests a significant possibility of ALPHAON® being able to provide endoscopic assistance.
Systematic Review and Meta-Analysis of Internal Jugular Vein Variants and Their Relationship to Clinical Implications in the Head and Neck
Authors: Nova-Baeza Pablo , Valenzuela-Fuenzalida Juan José , Valdivia-Arroyo Rocio , Becerra-Rodríguez Emelyn Sofia , Escalona-Manzo Catalina , Castaño-Gallego Yesica Tatiana , Luque-Bernal Ricardo Miguel , Oyanedel-Amaro Gustavo , Suazo-Santibáñez Alejandra , Orellana-Donoso Mathias , Bruna-Mejias Alejandro , Sanchis-Gimeno Juan , Gutiérrez-Espinoza Héctor
Published: December 2024

Background: The internal jugular vein (IJV) is a vascular structure that is responsible for the venous drainage of both the head and neck and is commonly found posterior to the internal carotid artery and adjacent to cervical lymph nodes or nerve structures such as the glossopharyngeal and accessory nerves. As a vagal nerve, it is an important reference point for surgical access in neck interventions and dissections. Methods: The databases Medline, Scopus, Web of Science, Google Scholar, CINAHL, and LILACS were searched until August 2024. Methodological quality was evaluated with an assurance tool for anatomical studies (AQUA). Pooled prevalence was estimated using a random effects model. Results: A total of 10 studies met the established selection criteria in this meta-analysis study. The prevalence of variants of the IJV was 3.36% (CI: 2.81–6.96%), with a heterogeneity of 94.46%. Regarding the subgroup analysis, no study presents statistically significant differences in the studies analyzed for this review. Conclusions: Knowing the IJV variants in detail will make early diagnosis useful, especially in surgeries in the neck region and in classic surgeries such as thyroidectomies and tracheostomies, among others. It will be important to know the position of the IJV.