bluuebunny/crossref_metadata_2025 huggingface dataset:
‘[T]his world is now thy pilgrimage’: William Michael Rossetti's Cognitive Maps of France and Italy
Authors: Sasso Eleonora
Published: March 2018

This paper takes as its starting point the conceptual metaphor ‘life is a journey’ as defined by Lakoff and Johnson (1980) in order to advance a new reading of William Michael Rossetti's Democratic Sonnets (1907). These political verses may be defined as cognitive-semantic poems, which attest to the centrality of travel in the creation of literary and artistic meaning. Rossetti's Democratic Sonnets is not only a political manifesto against tyranny and oppression, promoting the struggle for liberalism and democracy as embodied by historical figures such as Napoleon, Mazzini, Cavour, and Garibaldi; but it also reproduces Rossetti's real and imagined journeys throughout Europe in the late nineteenth century. This essay examines these references in light of the issues they raise, especially the poet as a traveller and the journey metaphor in poetry. But its central purpose is to re-read Democratic Sonnets as a cognitive map of Rossetti's mental picture of France and Italy. A cognitive map, first theorised by Edward Tolman in the 1940s, is a very personal representation of the environment that we all experience, serving to navigate unfamiliar territory, give direction, and recall information. In terms of cognitive linguistics, Rossetti is a figure whose path is determined by French and Italian landmarks (Paris, the island of St. Helena, the Alps, the Venice Lagoon, Mount Vesuvius, and so forth), which function as reference points for orientation and are tied to the historical events of the Italian Risorgimento. Through his sonnets, Rossetti attempts to build into his work the kind of poetic revolution and sense of history which may only be achieved through encounters with other cultures.
Efflux Activity Differentially Modulates the Levels of Isoniazid and Rifampicin Resistance among Multidrug Resistant and Monoresistant Mycobacterium tuberculosis Strains
Authors: Machado Diana , Perdigão João , Portugal Isabel , Pieroni Marco , Silva Pedro , Couto Isabel , Viveiros Miguel
Published: March 2018

With the growing body of knowledge on the contribution of efflux activity to Mycobacterium tuberculosis drug resistance, increased attention has been given to the use of efflux inhibitors as adjuvants of tuberculosis therapy. Here, we investigated how efflux activity modulates the levels of efflux between monoresistant and multi- and extensively drug resistant (M/XDR) M. tuberculosis clinical isolates. The strains were characterized by antibiotic susceptibility testing in the presence/absence of efflux inhibitors, molecular typing, and genetic analysis of drug-resistance-associated genes. Efflux activity was quantified by real-time fluorometry. The results demonstrated that all the M. tuberculosis clinical strains, susceptible or resistant, presented a faster, rapid, and non-specific efflux-mediated short-term response to drugs. The synergism assays demonstrated that the efflux inhibitors were more effective in reducing the resistance levels in the M/XDR strains than in the monoresistant strains. This indicated that M/XDR strains presented a more prolonged response to drugs mediated by efflux compared to the monoresistant strains, but both maintain it as a long-term stress response. This work shows that efflux activity modulates the levels of drug resistance between monoresistant and M/XDR M. tuberculosis clinical strains, allowing the bacteria to survive in the presence of noxious compounds.
Correcting the Input Data for Calculating the Asymmetry of Hydrogenic Spectral Lines in Plasmas
Authors: Sanders Paul , Oks Eugene
Published: March 2018

We provide corrections to the data in Sholin’s tables from his paper in Optics and Spectroscopy 26 (1969) 27. Since his data was used numerous times by various authors to calculate the asymmetry of hydrogenic spectral lines in plasmas, our corrections should motivate revisions of the previous calculations of the asymmetry and its comparison with the experimental asymmetry, and thus should have a practical importance.
Phenolic Compounds and Antioxidant Activity in Grape Juices: A Chemical and Sensory View
Authors: Cosme Fernanda , Pinto Teresa , Vilela Alice
Published: March 2018

The search for food products that promote health has grown over the years. Phenolic compounds present in grapes and in their derivatives, such as grape juices, represent today a broad area of research, given the benefits that they have on the human health. Grape juice can be produced from any grape variety once it has attained appropriate maturity. However, only in traditional wine producing regions, grape juices are produced from Vitis vinifera grape varieties. For example, Brazilian grape juices are essentially produced from Vitis labrusca grape varieties, known as American or hybrid, as they preserve their characteristics such as the natural flavour after pasteurisation. Grapes are one of the richest sources of phenolic compounds among fruits. Therefore, grape juices have been broadly studied due to their composition in phenolic compounds and their potential beneficial effects on human health, specifically the ability to prevent various diseases associated with oxidative stress, including cancers, cardiovascular and neurodegenerative diseases. Therefore, this review will address grape juices phenolic composition, with a special focus on the potential beneficial effects on human health and on the grape juice sensory impact.
Topical Digitoxigenin for Wound Healing: A Feasibility Study
Authors: Feng Xinchi , Wang Cuifen , Xu Yunhui , Turley Joel , Xie Zijian , Pierre Sandrine , Hao Jinsong
Published: March 2018

(1) Background: Cardiotonic steroids have been found to stimulate collagen synthesis and might be potential wound healing therapeutics. The objective of this study was to evaluate the feasibility of digitoxigenin and its topical formulation for wound healing; (2) Methods: In the in vitro study, the human dermal fibroblast cells were treated with digitoxigenin and collagen synthesis was assessed. In the in vivo study, digitoxigenin was applied to excisional full-thickness wounds in rats immediately after wounding and remained for three days, and wound open was evaluated over 10 days. A digitoxigenin formulation for topical administration was prepared, and the in vitro release and in vivo wound healing effect were investigated; (3) Results: The expression of procollagen in human dermal fibroblast was significantly increased with the exposure to 0.1 nM digitoxigenin. Topical application of digitoxigenin in olive oil or alginate solution for three days significantly decreased the wound open in rats. Similarly, topical administration of the developed digitoxigenin formulation for three days also significantly increased wound healing. No wound healing effects were observed at days 7 and 10 after wounding when digitoxigenin was not applied; and, (4) Conclusions: It was possible to deliver digitoxigenin using the developed formulation. However, the wound healing effect of digitoxigenin and its mechanisms need to be further investigated in future studies.
A Nanostructured Sensor Based on Gold Nanoparticles and Nafion for Determination of Uric Acid
Authors: Stozhko Natalia , Bukharinova Maria , Galperin Leonid , Brainina Khiena
Published: March 2018

The paper discusses the mechanism of uric acid (UA) electrooxidation occurring on the surface of gold nanoparticles. It has been shown that the electrode process is purely electrochemical, uncomplicated with catalytic stages. The nanoeffects observed as the reduction of overvoltage and increased current of UA oxidation have been described. These nanoeffects are determined by the size of particles and do not depend on the method of particle preparation (citrate and “green” synthesis). The findings of these studies have been used to select a modifier for carbon screen-printed electrode (CSPE). It has been stated that CSPE modified with gold nanoparticles (5 nm) and 2.5% Nafion (Nf) may serve as non-enzymatic sensor for UA determination. The combination of the properties of nanoparticles and Nafion as a molecular sieve at the selected pH 5 phosphate buffer solution has significantly improved the resolution of the sensor compared to unmodified CSPE. A nanostructured sensor has demonstrated good selectivity in determining UA in the presence of ascorbic acid. The detection limit of UA is 0.25 μM. A linear calibration curve has been obtained over a range of 0.5–600 μM. The 2.5%Nf/Au(5nm)/CSPE has been successfully applied to determining UA in blood serum and milk samples. The accuracy and reliability of the obtained results have been confirmed by a good correlation with the enzymatic spectrophotometric analysis (R2 = 0.9938) and the “added−found” technique (recovery close to 100%).
The Role of Oncogenic Tyrosine Kinase NPM-ALK in Genomic Instability
Authors: Lobello Cosimo , Bikos Vasilis , Janikova Andrea , Pospisilova Sarka
Published: March 2018

Genomic stability is crucial for cell life and transmitting genetic material is one of the primary tasks of the cell. The cell needs to be able to recognize any possible error and quickly repair it, and thus, cells have developed several mechanisms to detect DNA damage and promote repair during evolution. The DNA damage response (DDR) and DNA repair pathways ensure the control of possible errors that could impair the duplication of genetic information and introduce variants in the DNA. Endogenous and exogenous factors compromise genomic stability and cause dysregulation in the DDR and DNA repair pathways. Cancer cells often impair these mechanisms to overcome cellular barriers (cellular senescence and/or apoptosis), leading to malignancy. NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) is an oncogenic tyrosine kinase that is involved in the development of anaplastic large cell lymphoma (ALCL). NPM-ALK is known to be involved in the activation of proliferative and anti-apoptotic signaling pathways. New evidence reveals that NPM-ALK translocation also impairs the ability of cells to maintain the genomic stability through both DDR and DNA repair pathways. This review aims to highlight the role of the oncogenic tyrosine kinase NPM-ALK in the cell, and pointing to new possible therapeutic strategies.
Survival Analysis in Patients with Pancreatic Ductal Adenocarcinoma Undergoing Chemoradiotherapy Followed by Surgery According to the International Consensus on the 2017 Definition of Borderline Resectable Cancer
Authors: Hayasaki Aoi , Isaji Shuji , Kishiwada Masashi , Fujii Takehiro , Iizawa Yusuke , Kato Hiroyuki , Tanemura Akihiro , Murata Yasuhiro , Azumi Yoshinori , Kuriyama Naohisa , Mizuno Shugo , Usui Masanobu , Sakurai Hiroyuki
Published: March 2018

Background: The aim of this study was to validate a new definition of borderline resectable pancreatic ductal adenocarcinoma (PDAC) provided by the 2017 international consensus on the basis of three dimensions of anatomical (A), biological (B), and conditional (C) factors, using the data of the patients who had been registered for our institutional protocol of chemoradiotherapy followed by surgery (CRTS) for localized patients with PDAC. Methods: Among 307 consecutive patients pathologically diagnosed with localized PDAC who were enrolled in our CRTS protocol from February 2005 to December 2016, we selected 285 patients who could be re-evaluated after CRT. These 285 patients were classified according to international consensus A definitions as follows: R (resectable; n = 62), BR-PV (borderline resectable, superior mesenteric vein (SMV)/portal vein (PV) involvement alone; n = 27), BR-A (borderline resectable, arterial involvement; n = 50), LA (locally advanced; n = 146). Disease-specific survival (DSS) was analyzed according to A, B (serum CA 19-9 levels and lymph node metastasis diagnosed by computed tomography findings before CRT), and C factors (performance status (PS)) factors. Results: The rates of resection and R0 resection were similar between R (83.9 and 98.0%) and BR-PV (85.2 and 95.5%), but much lower in BR-A (70.0 and 84.8%) and LA (46.6 and 62.5%). DSS evaluated by median survival time (months) showed a similar trend to surgical outcomes: 33.7 in R, 27.3 in BR-PV, 18.9 in BR-A and 19.3 in LA, respectively. DSS in R patients with CA 19-9 levels > 500 U/mL was significantly poorer than in patients with CA 19-9 levels ≤ 500 U/mL, but there were no differences in DSS among BR-PV, BR-A, and LA patients according to CA 19-9 levels. Regarding lymph node metastasis, there was no significant difference in DSS according to each resectability group. DSS in R patients with PS ≥ 2 was significantly worse than in patients with PS 0-1. Conclusions: The international consensus on the definition of BR-PDAC based on three dimensions of A, B, and C is useful and practicable because prognosis of PDAC patients is influenced by anatomical factors as well as biological and conditional factors, which in turn may help to decide treatment strategy.
Evolution of Carbon Ion Radiotherapy at the National Institute of Radiological Sciences in Japan
Authors: Mohamad Osama , Makishima Hirokazu , Kamada Tadashi
Published: March 2018

Charged particles can achieve better dose distribution and higher biological effectiveness compared to photon radiotherapy. Carbon ions are considered an optimal candidate for cancer treatment using particles. The National Institute of Radiological Sciences (NIRS) in Chiba, Japan was the first radiotherapy hospital dedicated for carbon ion treatments in the world. Since its establishment in 1994, the NIRS has pioneered this therapy with more than 69 clinical trials so far, and hundreds of ancillary projects in physics and radiobiology. In this review, we will discuss the evolution of carbon ion radiotherapy at the NIRS and some of the current and future projects in the field.
Trends in Pediatric Complicated Pneumonia in an Ontario Local Health Integration Network
Authors: Haji Tahereh , Byrne Adam , Kovesi Tom
Published: March 2018

Following the introduction of 7-valent pneumococcal vaccine (PCV7), while overall rates of invasive pneumococcal disease and pneumococcal pneumonia in children declined, rates of empyema increased. We examined changes in the incidence of hospitalization for pediatric complicated pneumonia (PCOMP) in Eastern Ontario, Canada, particularly since the introduction of the 13-valent vaccine (PCV13). A retrospective chart review was carried out evaluating previously healthy children admitted with PCOMP, which included empyema, parapneumonic effusion, necrotizing pneumonia, and lung abscess between 2002 and 2015. Three-hundred seventy-one children were included. Subjects had a median age of four years, and 188/370 (50.8%) required a chest tube. Admission rates changed markedly during this time period. The number of admissions per year rose most sharply between 2009 and 2012, corresponding to the period following introduction of PCV7 and then the occurrence of pandemic influenza A (H1N1). In children who likely received PCV13, the incidence of PCOMP returned to approximately pre-PCV7 levels. In contrast, rates of PCOMP in older children (who would not have received PCV13) remained elevated during the post-PCV13 time period. While rates of PCOMP, particularly in older children, remain elevated following the introduction of PCV13, this might be expected to resolve with more widespread vaccine coverage with PCV13 and herd immunity.
Structural and Electronic Properties of (TMTTF)2X Salts with Tetrahedral Anions
Authors: Rösslhuber Roland , Rose Eva , Ivek Tomislav , Pustogow Andrej , Breier Thomas , Geiger Michael , Schrem Karl , Untereiner Gabriele , Dressel Martin
Published: March 2018

Comprehensive measurements of the pressure- and temperature-dependent dc-transport are combined with dielectric spectroscopy and structural considerations in order to elucidate the charge and anion orderings in the quasi-one-dimensional charge-transfer salts (TMTTF) 2 X with non-centrosymmetric anions X = BF 4 , ClO 4 and ReO 4 . Upon applying hydrostatic pressure, the charge-order transition is suppressed in all three compounds, whereas the influence on the anion order clearly depends on the particular compound. A review of the structural properties paves the way for understanding the effect of the anions in their methyl cavities on the ordering. By determining the complex dielectric constant ϵ ^ ( ω , T ) in different directions we obtain valuable information on the contribution of the anions to the dielectric properties. For (TMTTF) 2 ClO 4 and (TMTTF) 2 ReO 4 , ϵ b ′ exhibits an activated behavior of the relaxation time with activation energies similar to the gap measured in transport, indicating that the relaxation dynamics are determined by free charge carriers.
Thermodynamic Optimization for an Endoreversible Dual-Miller Cycle (DMC) with Finite Speed of Piston
Authors: Wu Zhixiang , Chen Lingen , Feng Huijun
Published: March 2018

Power output ( P ), thermal efficiency ( η ) and ecological function ( E ) characteristics of an endoreversible Dual-Miller cycle (DMC) with finite speed of the piston and finite rate of heat transfer are investigated by applying finite time thermodynamic (FTT) theory. The parameter expressions of the non-dimensional power output ( P ¯ ), η and non-dimensional ecological function ( E ¯ ) are derived. The relationships between P ¯ and cut-off ratio ( ρ ), between P ¯ and η , as well as between E ¯ and ρ are demonstrated. The influences of ρ and piston speeds in different processes on P ¯ , η and E ¯ are investigated. The results show that P ¯ and E ¯ first increase and then start to decrease with increasing ρ . The optimal cut-off ratio ρ o p t will increase if piston speeds increase in heat addition processes and heat rejection processes. As piston speeds in different processes increase, the maximum values of P ¯ and E ¯ increase. The results include the performance characteristics of various simplified cycles of DMC, such as Otto cycle, Diesel cycle, Dual cycle, Otto-Atkinson cycle, Diesel-Atkinson cycle, Dual-Atkinson cycle, Otto-Miller cycle and Diesel-Miller cycle. Comparing performance characteristics of the DMC with different optimization objectives, when choosing E ¯ as optimization objective, η improves 26.4% compared to choosing P ¯ as optimization objective, while P ¯ improves 74.3% compared to choosing η as optimization objective. Thus, optimizing E is the best compromise between optimizing P and optimizing η . The results obtained can provide theoretical guidance to design practical DMC engines.
Thermodynamic Analysis of an Irreversible Maisotsenko Reciprocating Brayton Cycle
Authors: Zhu Fuli , Chen Lingen , Wang Wenhua
Published: March 2018

An irreversible Maisotsenko reciprocating Brayton cycle (MRBC) model is established using the finite time thermodynamic (FTT) theory and taking the heat transfer loss (HTL), piston friction loss (PFL), and internal irreversible losses (IILs) into consideration in this paper. A calculation flowchart of the power output (P) and efficiency (η) of the cycle is provided, and the effects of the mass flow rate (MFR) of the injection of water to the cycle and some other design parameters on the performance of cycle are analyzed by detailed numerical examples. Furthermore, the superiority of irreversible MRBC is verified as the cycle and is compared with the traditional irreversible reciprocating Brayton cycle (RBC). The results can provide certain theoretical guiding significance for the optimal design of practical Maisotsenko reciprocating gas turbine plants.
Wind Predictions Upstream Wind Turbines from a LiDAR Database
Authors: Le Clainche Soledad , Lorente Luis , Vega José
Published: March 2018

This article presents a new method to predict the wind velocity upstream a horizontal axis wind turbine from a set of light detection and ranging (LiDAR) measurements. The method uses higher order dynamic mode decomposition (HODMD) to construct a reduced order model (ROM) that can be extrapolated in space. LiDAR measurements have been carried out upstream a wind turbine at six different planes perpendicular to the wind turbine axis. This new HODMD-based ROM predicts with high accuracy the wind velocity during a timespan of 24 h in a plane of measurements that is more than 225 m far away from the wind turbine. Moreover, the technique introduced is general and obtained with an almost negligible computational cost. This fact makes it possible to extend its application to both vertical axis wind turbines and real-time operation.
Annual Prediction Output of an RADTIRC-PV Module
Authors: Freier Daria , Muhammad-Sukki Firdaus , Abu-Bakar Siti , Ramirez-Iniguez Roberto , Munir Abu , Mohd Yasin Siti , Bani Nurul , Mas’ud Abdullahi , Ardila-Rey Jorge , Karim Md
Published: March 2018

The number of solar photovoltaic (PV) installations has been increasing worldwide but the high capital cost of installation continues to be the main challenge, particularly in many developing countries. The solar concentrator, a device that focuses the sunlight onto a small area, has the potential to minimize the use of expensive PV material while maintaining the system’s performance, ultimately bringing down its overall cost. This study aims to predict the annual electrical output of a specific concentrator design called the rotationally asymmetrical dielectric totally internally reflecting concentrator (RADTIRC). The aforementioned design is assumed to be installed in Berlin/Brandenburg, Germany. First, a short review of concentrators is provided. Next, a description of the RADTIRC and the previous research that revolved around it are provided. Afterwards, the key parameters that are needed to determine the annual electrical output of the RADTIRC are explained before presenting the results of the simulations. It was found that the yearly energy yield was increased by a factor of 2.29 when the RADTIRC-PV module was used when compared with the non-concentrating PV module.
Review of Wearable Device Technology and Its Applications to the Mining Industry
Authors: Mardonova Mokhinabonu , Choi Yosoon
Published: March 2018

This paper reviews current trends in wearable device technology, and provides an overview of its prevalent and potential deployments in the mining industry. This review includes the classification of wearable devices with some examples of their utilization in various industrial fields as well as the features of sensors used in wearable devices. Existing applications of wearable device technology to the mining industry are reviewed. In addition, a wearable safety management system for miners and other possible applications are proposed. The findings of this review show that by introducing wearable device technology to mining sites, the safety of mining operations can be enhanced. Therefore, wearable devices should be further used in the mining industry.
Methods of Decline Curve Analysis for Shale Gas Reservoirs
Authors: Tan Lei , Zuo Lihua , Wang Binbin
Published: March 2018

With help from horizontal wells and hydraulic fracturing, shale gas has made a significant contribution to the energy supply. However, due to complex fracture networks and complicated mechanisms such as gas desorption and gas slippage in shale, forecasting shale gas production is a challenging task. Despite the versatility of many simulation methods including analytical models, semi-analytical models, and numerical simulation, Decline Curve Analysis has the advantages of simplicity and efficiency for hydrocarbon production forecasting. In this article, the eight most popular deterministic decline curve methods are reviewed: Arps, Logistic Growth Model, Power Law Exponential Model, Stretched Exponential Model, Duong Model, Extended Exponential Decline Model, and Fractural Decline Curve model. This review article is dedicated to summarizing the origins, derivations, assumptions, and limitations of these eight decline curve models. This review article also describes the current status of decline curve analysis methods, which provides a comprehensive and up-to-date list of Decline Curve Analysis models for petroleum engineers in analysis of shale gas reservoirs. This work could serve as a guideline for petroleum engineers to determine which Decline Curve models should be applied to different shale gas fields and production periods.
Energy Flexometer: Transactive Energy-Based Internet of Things Technology
Authors: Babar Muhammad , Grela Jakub , Ożadowicz Andrzej , Nguyen Phuong , Hanzelka Zbigniew , Kamphuis I.
Published: March 2018

Effective Energy Management with an active Demand Response (DR) is crucial for future smart energy system. Increasing number of Distributed Energy Resources (DER), local microgrids and prosumers have an essential and real influence on present power distribution system and generate new challenges in power, energy and demand management. A relatively new paradigm in this field is transactive energy (TE), with its value and market-based economic and technical mechanisms to control energy flows. Due to a distributed structure of present and future power system, the Internet of Things (IoT) environment is needed to fully explore flexibility potential from the end-users and prosumers, to offer a bid to involved actors of the smart energy system. In this paper, new approach to connect the market-driven (bottom-up) DR program with current demand-driven (top-down) energy management system (EMS) is presented. Authors consider multi-agent system (MAS) to realize the approach and introduce a concept and standardize the design of new Energy Flexometer. It is proposed as a fundamental agent in the method. Three different functional blocks have been designed and presented as an IoT platform logical interface according to the LonWorks technology. An evaluation study has been performed as well. Results presented in the paper prove the proposed concept and design.
Study of Material Compatibility for a Thermal Energy Storage System with Phase Change Material
Authors: Qiu Songgang , Solomon Laura , Fang Ming
Published: March 2018

The suitability of stainless steel 316L and Inconel 625 for use in a latent heat thermal energy storage (TES) system was investigated. A NaCl–NaF eutectic mixture with a melting temperature of 680 °C was used as the phase change material (PCM). Containers were filled with the PCM prior to heating to 750 °C, then examined after 100 and 2500 h of high-temperature exposure by analyzing the material surface and cross-section areas. A small amount of corrosion was present in both samples after 100 h. Neither sample suffered significant damage after 2500 h. The undesirable inter-granular grain boundary attack found in SS316L samples was in the order of 1–2 µm in depth. On Inconel 625 sample surface, an oxide complex formed, resisting material dissolution into the PCM. The surface morphology of tested samples remained largely unchanged after 2500 h, but the corrosion pattern changed from an initially localized corrosion penetration to a more uniform type. After 2500 h, the corrosion depth of Inconel 625 remained at roughly 1–2 µm, indicating that the corrosion rate decelerated. Both materials demonstrated good compatibility with the chosen NaF–NaCl eutectic salt, but the low corrosion activity in Inconel 625 samples shows a performance advantage for long term operation.
Identification of Potential Metabolic Markers for the Selection of a High-Yield Clone of Quercus acutissima in Clonal Seed Orchard
Authors: Kang Jun , Lee Hyunseok , Lim Hyemin , Lee Wi
Published: March 2018

Quercus acutissima Carruth. is one of the most economically important deciduous tree species in Korea. The acorns of Q. acutissima are used for both food and medicinal purposes in Korea and China. In this study, we analyzed plant hormones and metabolite profiles to identify their correlation with the yield production of Q. acutissima. The contents of sucrose and inositol in the leaves of high-yield clones were significantly higher (p < 0.05) than those of low-yield clones. In addition, high-yield clones have a higher content of phosphoric acid, succinic acid, malic acid, and butane-1,3-diol in stems compared with low-yield clones. Among the identified metabolites, zeatin-9-glucoside showed highly significant negative correlations with tree height, crown volume, and acorn production. It is considered that these metabolites could be useful metabolic markers for the selection of a high yield clone of Q. acutissima.
Comparison of Geometric and Volumetric Methods to a 3D Solid Model for Measurement of Gully Erosion and Sediment Yield
Authors: Luffman Ingrid , Nandi Arpita , Luffman Benjamin
Published: March 2018

Gully erosion is a global problem that degrades land and reduces its utility for agriculture, development, and water quality. Quantification of sediment yield and control of sediment sources is essential for environmental protection. Five methods to evaluate erosion rates and sediment yield on an east Tennessee, USA, hillslope were compared: (1) physical measurement by removal of accumulated sediment using 10 L buckets; (2) repeated measurement of erosion pins in gully (erosional) and delta (depositional) areas; (3) geometric model using a combination trapezoidal prism-cylinder segment; (4) geometric model using a series of trapezoidal pyramids; and (5) 3D solid computer modeling. The 3D solid model created in SolidWorks was selected as the reference model and all other methods overestimated sediment yield to varying degrees. Erosion pin methods overestimated sediment yield by 368% in deltas and 123% in gullies. Volumetric measurement of sediment using buckets overestimated sediment yield by 160% due to void space in the buckets. The trapezoidal prism-cylinder segment model overestimated sediment yield by 66% and the trapezoidal pyramids method overestimated sediment yield by 5.7%. For estimation of sediment trapped behind an elliptical or circular silt fence dam, use of the trapezoidal pyramid method provides a good approximation comparable to 3D solid computer modeling.
Ambient Vibrations Measurements and 1D Site Response Modelling as a Tool for Soil and Building Properties Investigation
Authors: Imposa Sebastiano , Lombardo Giuseppe , Panzera Francesco , Grassi Sabrina
Published: March 2018

The safety of historic buildings heritage is an important task that becomes more substantial when the buildings are directed to educational purposes. The present study aims at evaluating the dynamic features of the Benedettini complex, an historic monastery located in downtown Catania, which is at present the headquarters of the humanistic studies department of the University of Catania. Both the building’s complex response to a seismic input and the soil-to-structure interaction were investigated using ambient noise recordings. The results point out a multiple dynamic behaviour of the monastery structure that shows several oscillation modes, whereas the identification of a single natural frequency can be observed in some sites where the structure can more freely oscillate. This observation is also confirmed by the variability of computed damping values that appear linked to the different rigidity of the structure, as a function of the either the longitudinal or transversal orientation of the investigated structural elements. Moreover, the comparison between the building’s fundamental period and spectral ratios frequencies, which were obtained from free field ambient noise measurements located outside the monastery, outline the presence of potential resonance effects between the site and structure during a seismic event. Numerical modelling of the local seismic response confirms the obtained experimental site frequencies, setting into evidence that higher amplification factors are reached in the same frequency range characterizing the building.
Toxicity of Beauveria bassiana-28 Mycelial Extracts on Larvae of Culex quinquefasciatus Mosquito (Diptera: Culicidae)
Authors: Vivekanandhan Perumal , Kavitha Thangaraj , Karthi Sengodan , Senthil-Nathan Sengottayan , Shivakumar Muthugoundar
Published: March 2018

Microbial-based pest control is an attractive alternative to chemical insecticides. The present study sought to evaluate the toxicity of the entomopathogenic fungus Beauveria bassiana-28 ethyl acetate extracts on different larval stages and pupae of Culex quinquefasciatus mosquitoes. B. bassiana-28 ethyl acetate mycelial extracts produced mosquitocidal activity against larvae and pupae which was comparable to that of the commercial insecticide B. bassiana-22 extract. The LC50 (lethal concentration that kills 50% of the exposed larvae) values of B. bassiana-28 extracts for 1st to 4th instar larvae and pupae were 11.538, 6.953, 5.841, 3.581 and 9.041 mg/L respectively. Our results show that B. bassiana-28 ethyl acetate mycelial extract has strong insecticidal activity against larval and pupal stages of Cx. quinquefasciatus. Fourier transform infrared spectrum study of B. bassiana-28 extract shows peaks at 3226.91; 2927.94; 1593.13; 1404.18; 1224.18; 1247.94; 1078.21; 1018.41; 229.69; and 871.82 cm−1. Major spectral peaks were observed at 3226.91 cm−1, assigned to N–H stretching, 2927.94 cm−1 assigned to C–H bonding and 1595.13 cm−1 assigned to C–O stretching. Gas Chromatography-Mass Spectrometry studies of B. bassiana-28 ethyl acetate crude extract showed presence of six major compounds viz. N-hexadecanoic acids (13.6040%); Z,Z-9,12 octadecadienic acid (33.74%); 9-eicosyne (10.832%); heptacosane (5.148%); tetrateracontane (5.801%); and 7 hexyleicosane (5.723%). Histology of mosquito midgut tissue shows tissue lysis as a result of B.bassiana-28 extract exposure. The study shows that bioactive molecules obtained from B. bassiana-28 mycelial extract has insecticidal properties and can be used as alternative for mosquito control.
Comparative Analysis of GF-1 and HJ-1 Data to Derive the Optimal Scale for Monitoring Heavy Metal Stress in Rice
Authors: Wang Dongmin , Liu Xiangnan
Published: March 2018

Remote sensing can actively monitor heavy metal contamination in crops, but with the increase of satellite sensors, the optimal scale for monitoring heavy metal stress in rice is still unknown. This study focused on identifying the optimal scale by comparing the ability to detect heavy metal stress in rice at various spatial scales. The 2 m, 8 m, and 16 m resolution GF-1 (China) data and the 30 m resolution HJ-1 (China) data were used to invert leaf area index (LAI). The LAI was the input parameter of the World Food Studies (WOFOST) model, and we obtained the dry weight of storage organs (WSO) and dry weight of roots (WRT) through the assimilation method; then, the mass ratio of rice storage organs and roots (SORMR) was calculated. Through the comparative analysis of SORMR at each spatial scale of data, we determined the optimal scale to monitor heavy metal stress in rice. The following conclusions were drawn: (1) SORMR could accurately and effectively monitor heavy metal stress; (2) the 8 m and 16 m images from GF-1 were suitable for monitoring heavy metal stress in rice; (3) 16 m was considered the optimal scale to assess heavy metal stress in rice.
Dynamic Relationships between Price and Net Asset Value for Asian Real Estate Stocks
Authors: LIOW Kim , YEO Sherry
Published: March 2018

This paper examines short- and long-term behavior of the price-to net asset value ratio in six Asian public real estate markets. We find mean-reverting behavior of the ratio and spillover effects, where each of the examined public real estate markets correlates with other markets. Additionally, the unexpected shock correlating with the price-to-net asset value ratio in one market has a positive or negative correlation with the ratios of other markets. Our results offer fresh insights to portfolio managers, policymakers, and academic researchers into the regional and country market dynamics of public real estate valuation and cross-country interaction from the long-term and short-term perspectives.
Chlorogenic Acid and Its Microbial Metabolites Exert Anti-Proliferative Effects, S-Phase Cell-Cycle Arrest and Apoptosis in Human Colon Cancer Caco-2 Cells
Authors: Sadeghi Ekbatan Shima , Li Xiu-Qing , Ghorbani Mohammad , Azadi Behnam , Kubow Stan
Published: March 2018

Chlorogenic acid (CGA) decreases colon cancer-cell proliferation but the combined anti-cancer effects of CGA with its major colonic microbial metabolites, caffeic acid (CA), 3-phenylpropionic acid (3-PPA) and benzoic acid (BA), needs elucidation as they occur together in colonic digesta. Caco-2 cancer cells were treated for 24 h with the four compounds individually (50–1000 µM) and as an equimolar ratio (1:1:1:1; MIX). The effective concentration to decrease cell proliferation by 50% (EC50) was lower for MIX (431 ± 51.84 µM) and CA (460 ± 21.88) versus CGA (758 ± 19.09 µM). The EC50 for cytotoxicity measured by lactate dehydrogenase release in MIX (527 ± 75.34 µM) showed more potency than CA (740 ± 38.68 µM). Cell proliferation was decreased by 3-PPA and BA at 1000 µM with no cytotoxicity. Cell-cycle arrest was induced at the S-phase by CA (100 µM), MIX (100 µM), CGA (250 µM) and 3-PPA (500 µM) with activation of caspase-3 by CGA, CA, MIX (500 and 1000 µM). Mitochondrial DNA content was reduced by 3-PPA (1000 µM). The anti-cancer effects occurred at markedly lower concentrations of each compound within MIX than when provided singly, indicating that they function together to enhance anti-colon cancer activities.
Miniaturization of the Clonogenic Assay Using Confluence Measurement
Authors: Mayr Christian , Beyreis Marlena , Dobias Heidemarie , Gaisberger Martin , Pichler Martin , Ritter Markus , Jakab Martin , Neureiter Daniel , Kiesslich Tobias
Published: March 2018

The clonogenic assay is a widely used method to study the ability of cells to ‘infinitely’ produce progeny and is, therefore, used as a tool in tumor biology to measure tumor-initiating capacity and stem cell status. However, the standard protocol of using 6-well plates has several disadvantages. By miniaturizing the assay to a 96-well microplate format, as well as by utilizing the confluence detection function of a multimode reader, we here describe a new and modified protocol that allows comprehensive experimental setups and a non-endpoint, label-free semi-automatic analysis. Comparison of bright field images with confluence images demonstrated robust and reproducible detection of clones by the confluence detection function. Moreover, time-resolved non-endpoint confluence measurement of the same well showed that semi-automatic analysis was suitable for determining the mean size and colony number. By treating cells with an inhibitor of clonogenic growth (PTC-209), we show that our modified protocol is suitable for comprehensive (broad concentration range, addition of technical replicates) concentration- and time-resolved analysis of the effect of substances or treatments on clonogenic growth. In summary, this protocol represents a time- and cost-effective alternative to the commonly used 6-well protocol (with endpoint staining) and also provides additional information about the kinetics of clonogenic growth.
EP4 Agonist L-902,688 Suppresses EndMT and Attenuates Right Ventricular Cardiac Fibrosis in Experimental Pulmonary Arterial Hypertension
Authors: Lai Ying-Ju , Chen I-Chen , Li Hsin-Hsien , Huang Chung-Chi
Published: March 2018

Right ventricular (RV) hypertrophy is characterized by cardiac fibrosis due to endothelial–mesenchymal transition (EndMT) and increased collagen production in pulmonary arterial hypertension (PAH) patients, but the mechanisms for restoring RV function are unclear. Prostanoid agonists are effective vasodilators for PAH treatment that bind selective prostanoid receptors to modulate vascular dilation. The importance of prostanoid signaling in the RV is not clear. We investigated the effects of the EP4-specific agonist L-902,688 on cardiac fibrosis and TGF-β-induced EndMT. EP4-specific agonist treatment reduced right ventricle fibrosis in the monocrotaline (MCT)-induced PAH rat model. L-902,688 (1 µM) attenuated TGF-β-induced Twist and α-smooth muscle actin (α-SMA) expression, but these effects were reversed by AH23848 (an EP4 antagonist), highlighting the crucial role of EP4 in suppressing TGF-β-induced EndMT. These data indicate that the selective EP4 agonist L-902,688 attenuates RV fibrosis and suggest a potential approach to reducing RV fibrosis in patients with PAH.
Integrated Molecular Characterization of Gastrointestinal Stromal Tumors (GIST) Harboring the Rare D842V Mutation in PDGFRA Gene
Authors: Indio Valentina , Astolfi Annalisa , Tarantino Giuseppe , Urbini Milena , Patterson Janice , Nannini Margherita , Saponara Maristella , Gatto Lidia , Santini Donatella , Do Valle Italo , Castellani Gastone , Remondini Daniel , Fiorentino Michelangelo , Von Mehren Margaret , Brandi Giovanni , Biasco Guido , Heinrich Michael , Pantaleo Maria
Published: March 2018

Gastrointestinal stromal tumors (GIST) carrying the D842V activating mutation in the platelet-derived growth factor receptor alpha (PDGFRA) gene are a very rare subgroup of GIST (about 10%) known to be resistant to conventional tyrosine kinase inhibitors (TKIs) and to show an indolent behavior. In this study, we performed an integrated molecular characterization of D842V mutant GIST by whole-transcriptome and whole-exome sequencing coupled with protein–ligand interaction modelling to identify the molecular signature and any additional recurrent genomic event related to their clinical course. We found a very specific gene expression profile of D842V mutant tumors showing the activation of G-protein-coupled receptor (GPCR) signaling and a relative downregulation of cell cycle processes. Beyond D842V, no recurrently mutated genes were found in our cohort. Nevertheless, many private, clinically relevant alterations were found in each tumor (TP53, IDH1, FBXW7, SDH-complex). Molecular modeling of PDGFRA D842V suggests that the mutant protein binds imatinib with lower affinity with respect to wild-type structure, showing higher stability during the interaction with other type I TKIs (like crenolanib). D842V mutant GIST do not show any actionable recurrent molecular events of therapeutic significance, therefore this study supports the rationale of novel TKIs development that are currently being evaluated in clinical studies for the treatment of D842V mutant GIST.
Insights into the Mechanisms of Chloroplast Division
Authors: Yoshida Yamato
Published: March 2018

The endosymbiosis of a free-living cyanobacterium into an ancestral eukaryote led to the evolution of the chloroplast (plastid) more than one billion years ago. Given their independent origins, plastid proliferation is restricted to the binary fission of pre-existing plastids within a cell. In the last 25 years, the structure of the supramolecular machinery regulating plastid division has been discovered, and some of its component proteins identified. More recently, isolated plastid-division machineries have been examined to elucidate their structural and mechanistic details. Furthermore, complex studies have revealed how the plastid-division machinery morphologically transforms during plastid division, and which of its component proteins play a critical role in generating the contractile force. Identifying the three-dimensional structures and putative functional domains of the component proteins has given us hints about the mechanisms driving the machinery. Surprisingly, the mechanisms driving plastid division resemble those of mitochondrial division, indicating that these division machineries likely developed from the same evolutionary origin, providing a key insight into how endosymbiotic organelles were established. These findings have opened new avenues of research into organelle proliferation mechanisms and the evolution of organelles.
SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma
Authors: Tsuge Minori , Osaki Mitsuhiko , Sasaki Ryo , Hirahata Mio , Okada Futoshi
Published: March 2018

Lung metastasis constitutes the leading cause of the death in patients with osteosarcoma. We have previously reported that plasminogen activator inhibitor-1 (PAI-1) regulates the invasion and lung metastasis of osteosarcoma cells in a mouse model and as well as in clinical samples. In the present study, we examined the anti-metastatic effect of SK-216, a small compound PAI-1 inhibitor, in human 143B osteosarcoma cells. An in vitro study showed that SK-216 treatment suppressed invasion activity by inhibiting PAI-1 expression in 143B cells, but had no influence on their proliferation or migration. 143B cells treated with SK-216 exhibited reduced matrix metalloproteinase-13 (MMP-13) secretion in a dose-dependent manner. Moreover, intraperitoneal injection of SK-216 into mouse models resulted in downregulation of PAI-1 expression levels in the primary tumors and showed suppression of lung metastases without influencing the proliferative activity of the tumor cells in the primary lesions. These results indicate that SK-216, a PAI-1 inhibitor, may serve as a novel drug to prevent lung metastasis in human osteosarcoma.
Endostatin Stimulates Proliferation and Migration of Myofibroblasts Isolated from Myocardial Infarction Model Rats
Authors: Sugiyama Akira , Hirano Yuka , Okada Muneyoshi , Yamawaki Hideyuki
Published: March 2018

Myofibroblasts contribute to the healing of infarcted areas after myocardial infarction through proliferation, migration, and production of extracellular matrix (ECM). Expression of endostatin, a cleaved fragment of type XVIII collagen, increases in the heart tissue of an experimental myocardial infarction model. In the present study, we examined the effect of endostatin on the function of myofibroblasts derived from an infarcted area. The myocardial infarction model was created by ligating the left anterior descending artery in rats. Two weeks after the operation, α-smooth muscle actin (α-SMA)-positive myofibroblasts were isolated from the infarcted area. Endostatin significantly increased the proliferation and migration of myofibroblasts in vitro. On the other hand, endostatin had no effect on the production of type I collagen, a major ECM protein produced by myofibroblasts. Endostatin activated Akt and extracellular signal-regulated kinase (ERK), and the pharmacological inhibition of these signaling pathways suppressed the endostatin-induced proliferation and migration. A knockdown of the COL18A1 gene in the myocardial infarction model rats using small interference RNA (siRNA) worsened the cardiac function concomitant with wall thinning and decreased the α-SMA-positive myofibroblasts and scar formation compared with that of control siRNA-injected rats. In summary, we demonstrated for the first time that endostatin might be an important factor in the healing process after myocardial infarction through the activation of myofibroblasts.
Electrospun Fibrous Scaffolds for Tissue Engineering: Viewpoints on Architecture and Fabrication
Authors: Jun Indong , Han Hyung-Seop , Edwards James , Jeon Hojeong
Published: March 2018

Electrospinning has been used for the fabrication of extracellular matrix (ECM)-mimicking fibrous scaffolds for several decades. Electrospun fibrous scaffolds provide nanoscale/microscale fibrous structures with interconnecting pores, resembling natural ECM in tissues, and showing a high potential to facilitate the formation of artificial functional tissues. In this review, we summarize the fundamental principles of electrospinning processes for generating complex fibrous scaffold geometries that are similar in structural complexity to the ECM of living tissues. Moreover, several approaches for the formation of three-dimensional fibrous scaffolds arranged in hierarchical structures for tissue engineering are also presented.