bluuebunny/crossref_metadata_2025 huggingface dataset:
EVALUATION OF BIOENHANCING EFFECT OF PIPERINE AND ITS ANALOGS ON THE PHARMACOKINETICS OF VERAPAMIL
Authors: Pharne Ashwini , Gabhe Satish
Published: December 2023

A present study was undertaken to understand the impact of Piperine and some of its derivatives on the bioavailability of verapamil used in the treatment of cardiac disorders. On oral administration, it undergoes fast biotransformation resulting in its low bioavailability. Its bioavailability varies between 20 % and 35 %. Piperine (a well-known bioenhancer) has been reported to improve the bioavailability of a wide range of structurally and therapeutically varied medicines. Hence some derivatives of piperine were synthesized and evaluated for their bioenhancing effect on verapamil. The bioenhancing effect of piperine and synthesized derivatives was investigated and compared using a validated HPLC method as per the USFDA guidelines. The pharmacokinetic characteristics of verapamil alone and when combined with piperine and its synthetic analogs were also investigated. When administered to Wistar rats, it was discovered that the bioavailability of verapamil was 1.54 times higher when given with piperine and it was 2.42 times higher when given with morpholine derivative. The study undertaken indicates that some synthetic derivatives of piperine can be successfully used to enhance the bioavailability of verapamil to a considerable extent.
MICRONEEDLES ASSISTED ENHANCEMENT OF TRANSDERMAL DELIVERY OF CURCUMIN THROUGH ORGANOGEL FORMULATION
Authors: Mulay Sushruta , Mehta D.
Published: December 2023

Curcumin is a natural compound found in turmeric and has been shown to have anti-inflammatory and antioxidant properties. Topical delivery of curcumin through an organic emulsion-based gel can effectively target inflammation at specific sites on the body. The current study aims to formulate Pluronic based organogel of curcumin, whose absorption is further assisted by microneedles technology. Nine organogel formulations were prepared and optimized by 32 full factorization model to fix the ratio of lecithin[A] and Pluronic[B] concentration in order to get optimum drug release after 2 hours (Q2) and maximum release of drug after 8 hours (Q8). The in-vitro study through the Franz diffusion cell indicated a significant impact of Pluronic F-127 concentration on the release of drug. As increased concentration of Pluronic increased the viscosity of formulations which ultimately retarded the release of drug. Based on Design of Experiment (DoE) study, optimized batch containing 6.5% lecithin and 20.6% Pluronic was further studied for microneedle assisted ex-vivo skin permeation through rat skin. Permeation without microneedle poration was around 70.26% after 8 hours, which was significantly increased (around 25%) after microneedle treatment of the skin. The results indicated promising application microneedle assisted skin permeation as a non-invasive and controlled delivery of curcumin through the transdermal route. Further, in-vivo studies are recommended to further establish this promising concept, targeting a variety of inflammatory conditions, including arthritis, psoriasis, and eczema, etc., through non-invasive transdermal drug delivery systems.
Design, Optimization and Characterization of Metformin Hydrochloride Loaded Biodegradable Microspheres using Box Behnken design for Local Delivery in Periodontitis
Authors: Gambhire Makarand S , Gambhire Vaishali , Kulkarni Abhijit , Dolas Ramdas , Muley Anuja , Bhide Manali
Published: December 2023

In the present study, metformin hydrochloride loaded microspheres were prepared for filling into the periodontal pockets with or without grafts for the treatment of periodontitis. For this purpose, chitosan was chosen as polymer and used at different drug/polymer ratios in preparation of microspheres by emulsion cross-linking method. Optimization was carried out by implementing three-factor three-level Box–Behnken design. Mathematical models were generated by regression analysis for responses of particle size (PS) and entrapment efficiency (EE). The experimental design took into account for the preparation of optimized formulation with maximum %EE and minimum PS at optimum process conditions for the microsphere formulation by reduction in chemical use and formulation time, in an economical way. The optimized formulation was selected on the basis of the desirability function and was further evaluated with respect to the particle size, entrapment efficiency, in vitro drug release, differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy and surface morphology studies. The results of release studies were evaluated kinetically and statistically. Particle size and entrapment efficiency of the selected batch were found to be in the range of 40.2- 59.6µm and 85- 95% respectively. The DSC studies revealed molecular dispersion and conversion of the drug into amorphous form. Surface morphology of microspheres was analyzed by scanning electron microscopy (SEM) and found to be spherical in shape with smooth surface. The mean particle size, EE, and in-vitro drug release of the optimized batch were found to be 51.4 ± 4.8µm, 96.5± 1.42%, and 79.8 ± 3.1% respectively. The release kinetics showed that the release followed the Peppas model, and the main mechanism of drug release was diffusion. These sustained release chitosan microspheres could be a promising drug delivery system for local delivery of metformin hydrochloride in the treatment of periodontitis.
Comparative Cardioprotective Effect of Herbs of Hridya dashemani (Karamarda- Carissa carandas L., Badara- Ziziphus jujuba Lam.) versus Arjuna-Terminalia arjuna (Roxb.ex.DC.) Wight&Arn. against Isoprenaline induced Myocardial Infarction in Rats
Authors: Rao Harini A , M S Veena , B R Lalitha , Bhat Sudhakara
Published: December 2023

Hridya dashemani (ten cardioprotective herbs) includes fruits of Amra, Amrataka, Lakucha, Karamarda, Vrukshamla, Amlavetasa, Kuvala, Badara, Dadima and Matulunga. In Ayurveda, these herbs have been known to be conducive to heart. The present study was designed to evaluate the cardioprotective action of two herbs of Hridya dashemani fruits of Karamarda (Carissa carandas L.) and fruits of Badara (Ziziphus jujuba Lam.)] on the basis of biochemical&histopathological parameters in isoprenaline (ISO) induced myocardial infarction (MI) in experimental rats and to compare with stem bark of Arjuna (Terminalia arjuna (Roxb.ex.DC.) Wight&Arn.), a well-known cardioprotective herb. Total 36 male wistar albino rats were randomly divided into six groups. Group I- normal control (NC), group II- ISO induced MI, group IIIpositive control with hesperidin (100 mg/kg b.w), group IV- Badara (Z. jujuba – 450 mg/kg b.w), group V- Karamarda (C. carandus – 450 mg/kg b.w), group VI- Arjuna (T. arjuna – 450 mg/kg b.w). After 21 days of pre-treatment, experimental MI was induced in all groups except NC by injecting ISO subcutaneously (85 mg/kg) on 19th&20th day at an interval of 24 hours. Serum biochemical parameters, including cardiac biomarkers and histopathological examination of heart tissues were evaluated. ISO treated rats had a significant (p < 0.05) elevation in serum levels of diagnostic marker enzymes (AST, ALT, CK-MB, LDH, ALP) when compared to NC. All the pre-treated groups had significantly (p < 0.05) reduced marker enzyme serum levels compared to the ISO treated control. The protective role of these herbs was further confirmed by histopathological examination. The comparison revealed that Karamarda (C. carandus) pre-treated had similar protective effect as Arjuna (T. arjuna) in various biochemical parameters (AST, ALT, ALP, LDH, CK-MB, HDL, cholesterol, creatinine). It may be concluded from the present study that 450 mg/kg b.w of water extract of the above herbs have cardioprotective action against isoprenaline induced MI, with Karamarda (C. carandus), Arjuna (T. arjuna) having almost similar effects. The study paves way to further evaluate these herbs as preventive and curative in cardiovascular disease (CVD) clinical management.
Computer-aided Design of New Hydroxamic Acid Derivatives Targeting the Plasmodium falciparum M17 Metallo-aminopeptidase with Favorable Pharmacokinetic Profile
Authors: Koné Moussa , N’Guessan Hermann , N'gouan Aka Joseph , Mansilla-Koblavi Frederica , MEGNASSAN EUGENE
Published: December 2023

Through structure-based molecular design, we virtually design new subnanomolar range antimalarial, inhibitors of Plasmodium falciparum M17 aminopeptidase (PfA-M17). We developed the complexation QSAR models from hydroxamic acid derivatives (HDA). A linear correlation was established between the computed Gibbs free energies of binding (GFE: ΔΔGcom) and observed enzyme inhibition constants (Kiexp) for each training set pKiexp = , R2 = 0.97. The predictive power of the QSAR model was validated with 3D-QSAR pharmacophore generation (PH4): pKiexp = 0.707×pKipred − 2.5182, R2 = 0.89. We then conducted a study on catalytic residues to exploit the different interactions (enzyme: inhibitor). Structural information from the models guided us in designing a virtual combinatorial library (VCL) of more than 56 thousand HDAs. The PH4 screening retained 48 new and potent HDAs with predicted inhibitory potencies pKipre up to 73 times lower than that of HDA1 (pKiexp = 2.5 nM). Combining molecular modeling and PH4 in-silico screening of the VCL resulted in the proposed novel potent antimalarial agent candidates with favorable pharmacokinetic profiles.
STUDY OF EPIDEMIOLOGY AND ITS PROGNOSTIC FACTORS FOR EXPECTED THERAPEUTIC MANAGEMENT OUTCOME ALONG WITH THE ANTIHYPERTENSIVE DRUG PATTERN FOR PREECLAMPSIA IN RURAL SETTING
Authors: Singh Shreya , Jogpal Vikas , Ray Rashmi , Sharma Abhishek
Published: December 2023

This study analyses the association between epidemiological factors and preeclampsia and the antihypertensive drug pattern used at different stages of preeclampsia. Total 252 patients were enrolled for study. Some of the study variables which were studied was maternal age, history of infertility, family income, education, antihypertensive drug used, body mass index of mother and baby’s birth weight. Study was performed SGT Hospital, Gurugram and was given ethical clearance by the Institutional Ethical Committee (IEC). Data was collected with the help of validated questionnaires. Occurrence of Pregnancy Induced Hypertension (PIH) in our study was found to be 47.2%. Maternal age, low socioeconomic status, less education level, >36 weeks of gestation are one of some major factors responsible for PIH. The current study implies that different dosage forms of labetalol was prescribed with P value <0.05.
Anti-Inflammatory Effects of Vitex altissima Leaf Extract in Lipopolysaccharide-Induced RAW 264.7 Macrophages.
Authors: Azeez Ajinza A. , RAUF Arun A. , Edwin Boby T.
Published: December 2023

Vitex altissima has been conventionally utilized for its wide-ranging properties in the management of oxidative stress and inflammation. The present investigation was centered on the quantification of the anti-inflammatory efficacy of Vitex altissima. The samples were evaluated for their ability to impede the activity of proteinase, denature proteins, and stabilize the membrane of human red blood cells (HRBC). The present study investigated the inhibitory impact of extracts on the production of total cyclooxygenase, lipoxygenase-5 (5-LOX), myeloperoxidase (MPO), and nitric oxide (NO) using the RAW 264.7 cell line. Furthermore, the antioxidant properties were assessed through the employment of both DPPH assay and reducing power assay. According to the findings, the methanolic extract of Vitex altissima (VAME) was identified as the most efficacious fraction with anti-inflammatory and antioxidant properties. The findings indicate that the extracts exhibited dose-dependent inhibition of proteinase, protein denaturation, and hemolysis of HRBC membrane, which is beneficial. The extracts of Vitex altissima, when treated at concentrations that are not cytotoxic, were observed to have a significant effect in reducing the activity of COX, 5-LOX, and MPO in RAW 264.7 cell line treated with LPS. This resulted in a decrease in NO levels. The dose-dependent increase in in vitro anti-inflammatory activity of Vitex altissima suggests its potential use as a pharmacological agent for the management of diseases related to inflammation. Additional comprehensive phytochemical investigations, in conjunction with in vitro and in vivo analyses, are necessary to identify the active constituent within the extract.
Anxiolytic activity of methanolic extract of Cardia dichotoma leaves in rats
Authors: Shendage Shalaka S. , Jadhav Sayali , Dhawale Kirankumar , Sawant Sameer H.
Published: December 2023

The present study aimed to evaluate the methanolic extract of Cordia dichotoma (MECD) leaves (MECD) for anxiolytic activity in rats. Elevated plus maze test (EPM) and light and dark test were used to evaluate the antianxiety property of MECD. Diazepam (2 mg/kg) and methanolic extract of Cordia dichotoma (50,100, and 200n mg/Kg, p.o.) were administered daily for 21 days. EPM and light and dark tests were assessed on the 7th, 14th, and 21st days. On the 21st day, animals were killed, and the striatum was excised for the estimation of various biochemical parameters like malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) as well as histopathological study. In this study, the (MECD) demonstrated a significant and dose-dependent increase in time spent in the open arm of the elevated plus maze (EPM) test and the time spent in the light compartment in the light & dark test. It also significantly decreased the MDA level and increased CAT, GSH, and SOD levels (p < 0.001) in the MECD-treated group compared to the control group. Furthermore, MECD significantly restored histological abnormalities, revealing reduced stress-induced damage. It is suggested that MECD possessed an anxiolytic effect via modulation of endogenous enzymes in experimental-induced anxiety in rats.
Preliminary pharmacological screening of Corchorus olitorius extracts for anti-inflammatory and wound healing activity
Authors: Pradhan Pratap Chandra , Kori Mohan Lal
Published: December 2023

Aim of present study was to investigate the preliminary screening of aerial part of Corchorus olitorius extracts for anti-inflammatory and wound-healing activity on experimental animals. Different extracts with respective solvents i.e. petroleum ether, chloroform, ethyl acetate, ethanol and aqueous extracts of C. olitorius were obtained by successive solvent extraction. All extracts were screened for anti-inflammatory activity using Xylene-induced ear edema in mice and wound healing effect was studied by using incision wound model in mice. The weight of the ear lobes and biochemical measurements, nitric oxide (NO) levels and MPO in the tissue sample were used to detect the anti-inflammatory effect. The wound healing effect was observed by measurement of tensile strength and protein and hydroxyproline level assessment in the healed tissues. Observations of present study were confirmed that ethanol extract of C. olitorius (EECO) was showed significant (p<0.05) inhibition in the ear edema of mice. NO and MPO activity was decreased significantly in EECO-treated mice. Wound healing potential was observed by significant improvement in tensile strength as well as protein and hydroxyproline level of healed tissue of EECO-treated mice. In conclusion, ethanol extract of C. olitorius was found most effective for anti-inflammatory through reduction of NO and MPO activity as well as wound healing potential in experimental mice.
A Novel Validated HS-GC-MS method for the trace level determination of Acetyl chloride as Isopropyl acetate in various anti-cancer drug substances
Authors: Kasina Srivalli , Nowduri Annapurna , Chavakula Ramdas
Published: December 2023

A novel, simple and precise headspace gas chromatography-mass spectrometry (HS-GC-MS) method was developed and validated for the determination of acetyl chloride (AC) in various anti-cancer drug substances like bendamustine HCl, Ibrutinib, trifluridine and abemaciclib drug substances. In this methodology, AC reacts with isopropyl alcohol and is converted into isopropyl acetate. Hence AC is quantified as Isopropyl acetate. The lower level of detection was achieved on the capillary GC column (DB-624, Fused silica capillary column; 30 m length; 0.32 mm internal diameter, coated with 6% Cyanopropylphenyl and 94% dimethyl polysiloxane stationary phase of 1.8 μm film thickness) with electron impact ionization (EI) technique by GC-MS in selective ion monitoring (SIM) mode. The mass ions were selected for the quantifier is 63 and the qualifier is 87 for isopropyl acetate. The performance of the method was assessed by evaluating the specificity, linearity, sensitivity, precision, accuracy and robustness experiments. The established limit of detection and limit of quantification values for the AC were 0.1 and 0.3 μg/g, respectively. This developed method was found to be linear with a correlation coefficient is greater than 0.999 for all four drug substances. The average recoveries of AC in bendamustine HCl, ibrutinib, trifluridine and abemaciclib were obtained 106.1, 109.0, 97.4 and 101.6%, respectively. The proposed method was validated successfully as per ICH guidelines. Hence, the proposed method can be routinely used for the quantification of AC in various anti-cancer drug substances.
Development and Validation of the Stability Indicating Assay Methodology employing LC-MS/MS for Concurrent Quantification of Dapagliflozin Propanediol Monohydrate and Metformin Hydrochloride: Probable Degradants Based on Mass Spectra
Authors: Patel Nikita S. , Patel Bhavesh H. , Kaushal Mona A.
Published: December 2023

For the concurrent measurement of Dapagliflozin Propanediol Monohydrate (DAPA) and Metformin Hydrochloride (MET) in combined dosage form, a quick, accurate, specific and easy Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) approach was created. The method was performed on a column C8 RRHD Eclipse (150 × 4.60 mm, 5 µm). 5 mM Ammonium Acetate Buffer pH-4.0, Methanol and Acetonitrile were the components of the mobile phase in the ratios of 30:65:05, respectively. The effluent was detected at 227 nm at a flow rate of 0.4 mL/min. The observed retention times for DAPA and MET were 7.297 and 3.230 min, respectively. The drug was stressed by being exposed to acid and alkali hydrolysis. From the mass spectra, it was found that two degradant peaks were observed in the standard mixture and the sample during alkali stress condition and probable degradants formed. The developed approach was validated in accordance with ICH guidelines. With correlation coefficients of 0.9969 for DAPA and 0.9975 for MET, it was discovered that the standard curve was linear over the range of 60-140 µg/mL and 300-700 µg/mL for DAPA and MET, respectively. The limit of detection (LOD) was 2.959 µg/mL and 8.893 µg/mL for DAPA and MET, respectively. The limit of quantification (LOQ) was 8.967 µg/mL and 26.949 µg/mL for DAPA and MET, respectively. The % recovery was determined in between 98 to 102%. The precision was within the limit (Relative Standard Deviation (RSD) <2%). The proposed stability indicating LC-MS/MS method can be successfully utilized for simultaneous estimation of DAPA and MET in combined dosage form without any prior separation of individual drugs and no interference was found due to degradant formed during stress condition.
Development And Validation Of RP-HPLC Method For Simultaneous Determination Of Aceclofenac And Piperine In Rat Plasma To Study Pharmacokinetic Parameters
Authors: Pharne Ashwini , Gabhe Satish Y.
Published: December 2023

The BCS Class II medication aceclofenac is poorly soluble in water and undergoes significant metabolism in human hepatocytes and microsomes. It has a 15% oral bioavailability, which is low. Piperine is a naturally occurring bioenhancer that is used to increase the oral bioavailability of many different medications. In this study, the pharmacokinetics of Aceclofenac (20 mg/kg) alone and in combination with Piperine (10 mg/kg) in rats were tested. Despite their distinct chemistry and physical properties, in this study, piperine was used to enhance the bioavailabilty of aceclofenac followed by the development and validation of HPLC method to determine their simultaneous measurment in rat plasma. Plasma samples were processed by solid phase extraction and subjected to HPLC analysis. The analysis identified retention time for Aceclofenac and piperine to be 5.3 and 14.4 minutes respectively. We found the linear concentration range of aceclofenac in rat plasma between 0.1 to 20 µg/mL. The correlation coefficient (R2) determined was 0.9995. Cmax was increased from 23.59 µg/mL to 48.52 µg/mL. By including piperine with aceclofenac in formulation, its oral bioavailability can be improved.
Expeditious Microwave-Assisted Synthesis of 1,3-Benzoxazoles Incorporating Substituted Thiazolidinone Moieties
Authors: Piste Pravina
Published: December 2023

The rapid synthesis of 3-[4-(1,3-benzoxazol-2-yl)phenyl]-2-aryl-1,3-thiazolidin-4-one 4(a-h) was achieved through microwave-assisted methods. This involved the reaction of Schiff bases derived from 2-(4-amino phenyl) benzoxazole 3(a-h) with ethanol, SHCH2COOH (thioglycolic acid) (1 mol, 0.92 mL), and ZnCl2 (5 mol%) under microwave irradiation (400W, 146°C) for 3-4 minutes, resulting in excellent yields. Compound precursors 3(a-h) were prepared from aromatic aldehydes and 2-(4-amino phenyl) benzoxazole in ethanol, followed by microwave irradiation for 1-2 minutes, with reaction progress monitored via TLC. The expeditious reaction time is a notable advantage of this protocol. Comprehensive structural elucidation of the synthesized compounds involved elemental analysis, IR, NMR, 13C NMR, and Mass spectroscopy. These newly synthesized compounds, 4(a-h), were subjected to in vitro antimicrobial screening against Staphylococcus aureus, Escherichia coli, and antifungal screening against Aspergillus Niger. The zone of inhibition in millimeters was measured using the cup plate method. Several compounds within the series exhibited substantial activity when compared to the standard drugs streptomycin and Fluconazole.
STUDIES ON FIXED DOSE COMBINATION OF IBRUTINIB AND QUERCETIN SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEMS IN HUMAN CANCER CELL LINES
Authors: Bagri Rashmi , Ravouru Nagaraju
Published: December 2023

Ibrutinib (IB), irreversibly inhibits Bruton’s Tyrosine Kinase which plays a crucial role in the tumor microenvironment and Quercetin (QC) shown apoptosis induction, angiogenesis inhibition, and anti-proliferative action against several human carcinoma cells. The self nano-emulsifying drug delivery system (SNEDDS) is suitable for the loading of insolubilized oil-based compounds such as Ibrutinib and Quercetin. In the current study IB with QC was formulated into SNEDDS and cytotoxicity was determined by using Human malignant melanoma (A-375) and Human lung adenocarcinoma (A549) cell lines. The optimized loaded formula consisted of Castor oil, Kolliphor® RH 40, and PEG-600. The optimized formulation was evaluated for physical parameters and the results were satisfactory. For cytotoxicity studies MTT assay was conducted for these combinations, IC50 values were calculated for the tested compound. In A-549 adenocarcinoma cell line, the calculated IC50 values (μM) for the test compounds T1 (pure IB+QC) and T2 (IB+QC SNEDDS) were 70.34+ 0.8 µM and 85.46 + 0.93 µM at 24 h study, respectively. In A-375 cancer cell line, IC50 values for the compounds T1 and T2 were 59.52 + 0.87 µM and 88.43 + 1.03 µM for 24 h study, respectively. It was observed that the IC50 of IB-QC loaded SNEDDS was higher than pure drug combination and these enter the cells by active transport and induce cytotoxicity to the cells. The overall results from the studies suggest that IB-QC-loaded SNEDD provided synergistic effects, which could play a significant role in the percentage of cell death.
In-silico Studies of Heterocyclic Benzoxazole Derivatives as an Anticancer Agent: Molecular Docking, 2D and 3D QSAR
Authors: Pawar Smita J. , Zope Dhanashri , Kale Amol P.
Published: December 2023

In-silico molecular docking studies and QSAR study of benzoxazole derivatives synthesized by Kakkar et al. was done. Comparative studies of docking of 5-flurouracil and 20 compounds revealed presence of considerable interactions which indicates the affinity of newly synthesized compounds for thymidylate synthase. The statistically significant 2D-QSAR models were developed using molecular design suite (VLifeMDS 4.6). The study was performed with 20 compounds (data set) using sphere exclusion (SE) algorithm, random selection and manual selection methods used for the division of the data set into training and test set. Multiple linear regression [MLR] methodology with stepwise (SW) forward-backward variable selection method was used for building the QSAR models. The results of the 2D-QSAR models were further compared with 3D-QSAR models generated by kNN-MFA, (k-Nearest Neighbor Molecular Field Analysis) investigating the substitutional requirements for the favourable anticancer activity against HCT 116 cell line and providing useful information in the characterization and differentiation of their binding sites. The results derived may be useful for further designing of benzoxazole derivatives as anticancer agents prior to synthesis.
Molecular Docking Studies to Identify Inhibitors of Norepinephrine Reuptake from Marine Algae
Authors: Razvi Ummehani A. , Kamble Laxmikant H.
Published: December 2023

Depression is one of the major mental health problems at prevalence now days. It can be characterized by poor concentration, low self-esteem, losing interest in family or social life, feeling tired or fatigue, suicidal thoughts and similar symptoms. There are treatments like psychotherapies, antidepressants and electroconvulsive therapies available, but there is need to identify more effective treatment with lesser side effects. Marine organisms like algae, sponges or corals are been investigated to explore their potential as antidepressants. This article aims to explore the potential of some compounds from marine algae by molecular docking and assessment of pharmacokinetics. Human norepinephrine transporter (hNET) was used as target for this study as this transporter is responsible to reuptake norepinephrine and disturbs chemical balance of brain, which can be a cause for depression. Binding affinity of these compounds was compared with binding affinity of prescribed drug levomilnacipran. From 14 selected compounds, 13 showed higher binding affinity towards hNET. Among all compounds, saringosterone has highest binding score. Pharmacokinetics properties were constructive for most compounds. Compounds showed weaker druglikeness and drugs score but can be optimized to enhance it.  Compounds identified as inhibitors of NET can be developed as drug molecules in future or algal source for it can be taken as a food supplement.
Percepções sobre comportamento alimentar compulsivo, imagem corporal e inflamação em indivíduos com esquizofrenia
Authors: Amato Isabel de Andrade , Silva Renato Oliveira , Lorencetti Pedro Gabriel , Vicentin Sérgio Roberto Correa , Prado Thaís Paulino do , Bobbo Vanessa Cristina , Nogueira Guilherme Augusto da Silva , Celeri Eloisa Helena Rubello Valler , Azevedo Renata Cruz Soares de , Velloso Lício Augusto , Dalgalarrondo Paulo , Santos Junior Amilton dos
Published: December 2023

Objetivo: O Transtorno da Compulsão Alimentar Periódica (TCAP) é altamente prevalente em indivíduos com esquizofrenia. Este estudo tem como objetivo explorar a percepção da compulsão alimentar em pacientes com esquizofrenia e suas associações com marcadores clínicos, psicopatológicos e inflamatórios. Métodos: 45 participantes com queixa de compulsão alimentar foram avaliados por meio da Escala de Compulsão Alimentar Periódica (BES). Medidas clínicas e antropométricas, gordura corporal, dimensões psicopatológicas (PANSS) e um escore de satisfação com a imagem corporal com base nos itens da Body Shape Satisfaction Scale (BSSS) e do Body Shape Questionnaire (BSQ) e marcadores inflamatórios séricos, como IL-1β, IL - 6, IL-10, TNF-α e leptina foram dosados. Resultados: Os testes de correlação de Spearman revelaram correlações positivas entre escores de BES, insatisfação com a imagem corporal e escore PANSS, bem como correlação negativa com IL-10. Modelos de regressão linear múltipla mostraram que os escores de BES, embora não tenham sido suficientes para o diagnóstico de compulsão alimentar, estiveram associados positivamente com escore de insatisfação com a imagem corporal, níveis de IL-1β, IL-6 (ambas interleucinas pró inflamatórias) e negativamente com IL-10 (uma interleucina anti-inflamatória). Conclusão: Nossos achados sugerem que a inflamação crônica e a psicopatologia em indivíduos com esquizofrenia podem estar associados a comportamentos alimentares compulsivos e pior percepção de autoimagem corporal. Além da inflamação, o comer compulsivo pode contribuir para o aumento da mortalidade cardiovascular e piora do estado de saúde desses pacientes.
Stability of the protactinium(V) mono-oxo cation probed by first-principle calculations
Authors: Shaaban Tamara , Réal Florent , Maurice Rémi , Vallet Valérie
Published: December 2023

Protactinium (Z = 91) exhibits a distinct behaviour among the actinides. Unlike its neighbors uranium, neptunium or plutonium in the pentavalent oxidation state, it does not form the dioxo protactinyl moiety PaO2^{+}, but it can exist in the form of a monooxo PaO^{3+} cation. In this article, with the standard first-principle calculations and by including implicit and explicit solvation, we investigate two stoichiometrically equivalent neutral complexes PaO(OH)2(X)(H2O) and Pa(OH)4(X) where X is a monodentate ligand (X = OH-, F-, Cl-, Br-, I- and NCS-), as well as bidentate ligands (X = NO3^{-}, SO4^{2-}, and C2O4^{2-}). We evaluate the relative stability of the complexes by calculating the Gibbs' free energy of the reaction PaO(OH)2(X)(H2O) -> Pa(OH)4(X). The PaO(OH)2(X)(H2O) complex is stabilized with Cl-, Br-, I-, NCS-, NO3^{-} and SO4^{2-} ligands, while it is not energetically favored with OH-, F- and C2O4^{2-} ligands, implying the stability of the Pa(OH)4(X) complexes over PaO(OH)2(X)(H2O) ones. Moreover, the analysis utilizing the QTAIM (Quantum Theory of Atoms in Molecules) and NBO (Natural Bond Orbital) methods confirms that the Pa mono-oxo bond is a triple bond with significant contributions from the 5f and 6d shells to the bonding orbitals. The covalency of the Pa mono-oxo bond in the PaO(OH)2(X)(H2O) complexes increases with the Cl-, Br-, I-, NCS- and NO3^{-} ligands. These findings shed light on the unique chemical behavior of protactinium and offer valuable insights into the specific experimental conditions necessary for their stability.
Quantifying Electrochemical Degradation in Single-crystalline LiNi0.8Mn0.1Co0.1O2–Graphite pouch cells through Operando X-ray and Post-mortem Investigations
Authors: S. Menon Ashok , Shah Nickil , A. Gott James , Fiamegkou Eleni , J. W. Ogley Matthew , J. Paez Fajardo Galo , Vaenas Naoum , Ellis Ieuan , Ravichandran Nishitha , Cloetens Peter , Karpov Dmitry , Warnett Jay , Malliband Paul , Walker David , West Geoff , Loveridge Melanie , Piper Louis
Published: December 2023

Layered nickel-rich lithium transition metal oxides (LiNixMnyCo1−x−yO2; where x ≥ 0.8), with single-crystalline morphology, are promising future high-energy-density Li-ion battery cathodes due to their ability to mitigate particle-cracking-induced degradation. This is due to the absence of grain boundaries in these materials, which prevents the build-up of bulk crystallographic strain during electrochemical cycling. Compared to their polycrystalline counterparts, there is a need to study single-crystalline Ni-rich cathodes using operando X-ray methods in uncompromised machine-manufactured industry-like full cells to understand their bulk degradation mechanisms as a function of different electrochemical cycling protocols. This can help us identify factors to improve their long-term performance. Here, through in-house operando X-ray studies of pilot-line-built LiNi0.8Mn0.1Co0.1O2–Graphite A7 pouch cells, it is shown that their electrochemical capacity fade under harsh conditions (2.5–4.4 V and 40 °C for 100 cycles at C/3 rate) primarily stems from the high-voltage reconstruction of the cathode surface from a layered to a cubic (rock salt) phase that impedes Li+ kinetics and increases cell impedance. Post-mortem electron and X-ray microscopy show that these cathodes can withstand severe anisotropic structural changes and show no cracking when cycled under such conditions. Comparing these results to those from commercial Li-ion cells with surface-modified single-crystalline Ni-rich cathodes, it is identified that cathode surface passivation can mitigate this type of degradation and prolong cycle life. In addition to furthering our understanding of degradation in single-crystalline Ni-rich cathodes, this work also accentuates the need for practically relevant and reproducible fundamental investigations of Li-ion cells and presents a methodology for achieving this.
In-group Unity in Anglophone Cameroon's Separatist Discourse. Strategies and Means of Realization
Authors: Echitchi Raymond
Published: December 2023

The present paper is the result of a scientific venture into the discursive construction of separatism in Cameroon’s English-speakingregions, which separatists refer to as Southern Cameroons or Ambazonia. My study aims specifically at identifying the linguistic and rhetorical strategies separatist leaders use in order to create a sense of unity amongst all English-speaking Cameroonians and make them join the fight for independence. To achieve this aim, I have analysed speeches authored between 2010 and 2015 by two Anglophone Cameroonian separatist leaders. The analysis of the speeches, which followed the Discourse Historical Approach (Wodak et al. 2009), reveals that Southern Cameroonian nationalists try to achieve unity by resorting to strategies such as nomination, predication, and argumentation through topoi. These strategies were realised by means of linguistic resources including lexicon, imperatives, deontic and epistemic modals.
In Vitro Callus Induction and Adventitious Shoot Regeneration from Leaf Explants of Heuchera Hybrid
Authors: Muratova S. A. , Khoroshkova Yu. V.
Published: December 2023

The problems of morphogenesis’ induction from isolated leaf explants of the variety Georgia Plum of Heuchera hybrid were studied for the first time. The induction of callus and of adventitious shoots from leaf explants on the modified MS medium containing 30g/l glucose was carried out in the presence of 6-BAP in combination with one of the auxins IAA, IBA, NAA or 2,4-D. The cytokinin: auxin ratio in the medium was 10:1 or 20:1. The best regeneration of Heuchera hybrid (up to 92% of regenerating leaf discs) was obtained on media containing 6-BAP at a concentration of 4.0 mg/l in combination with NAA at a concentration of 0.2 mg/l or 0.4 mg/l. In this case, the number of adventitious shoots per regenerating disc could reach 7‑9 and more. The maximum frequency of direct regeneration was observed on media containing IBA. Abundant callus formation was observed on media containing 2,4-D.
Role of Manganese in Calcium Metabolism at the Level of the Enteral and Internal Environment of the Body
Authors: Ksenofontov D. A. , Voinova O. A. , Ksenofontova A. A. , Sakovtseva T. V.
Published: December 2023

The authors studied the effect of manganese on calcium metabolism at the level of the enteral and internal environment of the body in an experiment on rabbits. Two groups (control and experimental) of rabbits selected by the paired-analysis method were used in the experiment. There were three animals in each group. The animals in the experimental group were given a diet containing three times the amount of manganese. At the end of the experiment, blood was collected from various parts of the digestive tract of all animals under general anaesthesia. Organs and tissues werecollected for analysis after control slaughter, and the intestines were removed, followed by sampling of chyme and mucosa from the walls of various sections. The chyme was separated into fractions using the developed method. The concentration of manganese and calcium in intestinal chyme and its fractions, in mucosa of the intestinal wall, in the blood flowing in and out of the digestive tract, in organs and tissues was determined by the atomic absorption spectrophotometry method. It was shown experimentally that when the concentration of manganese in the diet of rabbits was tripled, the element was not accumulated in the internal environment but was accumulated by endogenous structures of the chyme and the mucous membrane of the wall of the small and large intestines. A metabolic change in calcium metabolism was revealed when manganese was tripled in the diet: its concentrations in the blood flowing from the intestines and in the mucous layer of the small intestine wall decreased. As a result, there was a significant decrease in bone concentrations and a downward trend in liver and kidney concentrations. The antagonistic effect of manganese on calcium metabolism in the enteric and internal environment is concluded. In the interaction of elements, the leading role of endogenous structures of chyme is noted due to ion-exchange properties of glycoproteins of intestinal cavity mucosa.
Physiological Aspects of Interaction of Nanoparticles with Plant and Microorganism Cells
Authors: Khlebnikova D. A. , Polivanova O. B. , Boytsova M. V. , Chepovoy I. I. , Munkhbaatar N.-O. , Cherednichenko M. Yu.
Published: December 2023

Nanoparticles (NPs) are materials with unique physical and chemical properties that are less than 100 nm in size. They are widely used in various fields of industry, medicine and agriculture. In agribusiness nanomaterials are used as nanofertilisers and nanopesticides. This fact requires a detailed study of the physiological, biochemical and molecular genetic responses of cells of living organisms – plants, fungi and animals – to interaction with nanomaterials. This review article provides information on the mechanisms of nanoparticle absorption, movement and molecular interaction in plant organisms, as well as mechanisms of their antibacterial and fungicidal activity. Available scientific resources devoted to the physiological features of nanoparticle absorption by plants indicate two possible ways of their penetration into the plant organism – apoplastic and symplastic. In plant cells, nanoparticles act as reactive oxygen species (ROS), causing oxidative stress and triggering enzymatic and non-enzymatic defence systems that result in both inhibition of physiological processes and stimulation of plant growth and development and, consequently, increased yield. The effect on the plant organism is species-specific and depends on the type of nanomaterial and its concentration. Detailed laboratory and field studies are required to determine the specific effect of nanomaterials on a particular plant species, while complying with all toxicological safety standards to avoid environmental contamination with nanomaterials. Nanoparticles act on microorganism cells as physical and chemical disruptors – they change the permeability of cell walls and organelle membranes, protein configuration, damage DNA, leading to physical destruction of cells. Such properties of nanoparticles define antimicrobial and fungicidal activities of nanoparticles. However, nanoparticles should be used cautiously in crop production, as both plant life and productivity depend largely on microbial symbionts, and their effect on microbial cells is not species-specific.
СУБ’ЄКТИВНЕ БЛАГОПОЛУЧЧЯ ЯК ЧИННИК СОЦІАЛЬНО-ПСИХОЛОГІЧНОЇ АДАПТАЦІЇ ОСОБИСТОСТІ В УМОВАХ ВІЙНИ
Authors: Малімон Людмила , Пашкіна Алла , Максимчук Олександр
Published: December 2023

Мета. Встановити особливості вираженості психологічного благополуччя особистості в умовах військових дій та його зв’язок з характеристиками соціально-психологічної адаптації.
Методи. Методи теоретичного аналізу проблеми; емпіричного дослідження за допомогою комплексу психодіагностичних методик; методи математичної статистики: відсотковий і факторний аналіз.
Результати. Виявлено домінуючі характеристики соціально-психологічної адаптації осіб з різним рівнем психологічного благополуччя. Для осіб з низьким рівнем психологічного благополуччя характерне використання копінг-поведінки (як засобу подолання невизначеності та стресової ситуації) на засадах самоконтролю, втечі-уникнення та планування власних дій щодо проблемної ситуації, у той час як в осіб з високим рівнем психологічного благополуччя суттєво переважає стратегія прийняття відповідальності. В осіб з високим рівнем психологічного благополуччя високий показник загальної соціальної адаптованості формується завдяки широті охоплень сигналів соціуму, готовності до конструктивних дій задля досягнення цілей; в осіб з низьким рівнем психологічного благополуччя найнижчі показники за критеріями стійкість емоційного переживання та точність орієнтації в соціальних очікуваннях,
За допомогою факторного аналізу виявлено факторну структуру симптомокомплексів соціально-адаптаційних характеристик респондентів з низьким, середнім та високим рівнями психологічного благополуччя.
Висновки. В осіб з низьким рівнем психологічного благополуччя переважають характеристики адаптації, які вказують більшою мірою на емоційне реагування (домінування прагнення до емоційного комфорту); в осіб з середнім рівнем психологічного благополуччя виражене ситуативне застосування поведінкових та когнітивних стратегій; в осіб з високим рівнем психологічного благополуччя суттєво переважає раціональний поведінковий компонент в характеристиках соціально-психологічної адаптації.
НЕВИЗНАЧЕНА ВТРАТА У КОНТЕКСТІ ВИМУШЕНОЇ СЕПАРАЦІЇ ТА ВИМУШЕНОГО УСИНОВЛЕННЯ
Authors: Розмирська Юлія , Глова Ірина
Published: December 2023

Purpose. The article provides a theoretical overview of the theory of ambiguous loss in the focus of physical absence with the psychological presence of a loved one.
Methods. To achieve the purpose of the study, the methods of scientific theoretical study were used: analysis, synthesis, structuring, and generalization.
Results. Based on the analysis of research on the forced separation of children from their families and its psychological consequences, we can state that the theory of ambiguous loss is important in the situation of forced adoption of illegally displaced children. The theory of ambiguous loss clearly explains the mechanism of experiencing separation from a loved one, when this separation is not fully understood - there is no clear knowledge of the person's status: alive or dead, or the person is physically present but mentally absent, as in the case of dementia. The theory of ambiguous loss clearly explains three types of consequences: immobilization (affects the ability to communicate), consequences at the level of relationships (conflicts due to different views of the situation) and on the personality (manifestations of such conditions as depression, anxiety, guilt, etc.).
Conclusions. The model of finding resilience in the face of uncertain loss includes such interrelated structural blocks as finding meaning, tempering mastery, reconstructing identity, normalizing ambivalence, revising attachment and discovering hope. The experience of working with displaced children in El Salvador is also important, because of studying the «disappearance» and "reunification" stages. They formed the basis for adapting the model of resilience in ambiguous loss to study the psychological consequences of forced adoption. The prospects for further research are in the empirical study of the psychological consequences of the forced adoption traumatic situation for children and their families.
СПІЛЬНА РЕЗИЛЬЄНТНІСТЬ У СПІЛЬНІЙ ТРАВМАТИЧНІЙ РЕАЛЬНОСТІ ПРАКТИЧНИХ ПСИХОЛОГІВ: ДОСВІД УКРАЇНИ ТА НАЙКРАЩІ ПРАКТИКИ ЄВРОПЕЙСЬКОГО СОЮЗУ
Authors: Засєкіна Лариса , Бурцан Володимир
Published: December 2023

Purpose. The study aims to theoretically and empirically study the shared resilience in applied psychologists at the beginning of their professional career, as well as to study the best practices of the European Union for the development of shared resilience.
Methods. The study applies a set of standardized measures: the Shared Traumatic and Professional Posttraumatic Growth Scale (STPPG) (Tosone 2016); the Readiness to Work with Trauma-Exposed Patients Scale (RTEPS) (Kazlauskas 2022); Brief Resilient Coping Scale (BRCS) (Sinclair & Wallston, 2004); Conjoint Community Resilience Assessment Measure (CCRAM) (Leykin et al. , 2013). Statistical processing of the data was carried out using descriptive statistics, regression and correlation analysis of the software tool IBM SPSS Statistics, 2023. The aprticipants were represented by a sample of senior students (n=85) of the Faculty of Psychology at Lesya Ukrainka Volyn National University who had clinicician’s or psychologist’s professional experience. The survey was carried out in September 2023 and was conducted online through a link on Google Forms.
Results. The results indicate that shared is positively associated with shared trauma, professional post-traumatic growth and readiness to work with trauma.The results of the regression analysis show that shared trauma, posttraumatic growth, volunteering, having children, and personal experience of getting therapy are predictors of shared resilience.
Conclusions. Moreover, learning and implementing the best practices of European Union to develop the applied psychologists’ shared resilience is of crucial importance in the context of shared traumatic reality in Ukraine.
The Institute of insolvency (bankruptcy) and the legal status of the arbitration manager: a comprehensive intersectoral analysis
Authors: Zakomoldin Aleksey
Published: December 2023

The monograph deals with the current problems of legal regulation and application of the institution of insolvency (bankruptcy) in the present days. The author analyzes legal status of the arbitration manager as one of the key subjects of anti-crisis management. Special attention is paid to the criminal law analysis of crimes in the field of bankruptcy according to the current Criminal Code of the Russian Federation.
The publication is intended for students and teachers of the legal, economic and management studies, arbitration managers, judges, law enforcement officers, as well as all persons interested in the problems of the crisis management.
Analysis of water quality on growth performance bamboo lobster (Panulirus versicolor) in Tanjung Limau, Bontang City, East Kalimantan
Authors: Andriawan Soni , Fahreza Mohammad Adam , David Hermawan , Handajani Hany
Published: December 2023

Lobster is one of the commodities that have high economic value in the world market. Thus, the export opportunities for lobster commodities are still promising. One area that can produce lobster in Indonesia is Bontang, East Kalimantan. The area which is the sea waters is very potential for lobster rearing cultivation. This study was conducted with the aim of analyzing good water quality in lobster rearing cultivation in Bontang, East Kalimantan. The study used 3 floating net cages by taking water samples 4 times and 5 lobsters each sampling. Water quality samples include physics (color, smell and taste, temperature, brightness), chemistry (pH, DO, nitrite, salinity), biology (coliform), heavy metals (hg, pb, cu). Lobster samples were measured by weight. The results showed that Fsignificant > 0.05 so that the water quality parameters had no effect on growth. The cause of the ineffectiveness of water quality parameters is due to the contamination of waters in Bontang, East Kalimantan.Keywords: Bamboo Lobster; Contamination; Floating Net Cage; Heavy Metal.