bluuebunny/crossref_metadata_2025 huggingface dataset:
Trouton-Noble-like experiments on suspended capacitors
Authors: Porcelli Elio B , Filho Victo S
Published: January 2021

We performed several measurements of angular velocities on a suspended monolayer capacitor subjected to high electric voltages. The experimental setup is similar to that reported by Trouton and Noble, but which presented negative results for the rotation of the device. The measurements were made without or with electromagnetic shielding in the capacitor. We measured a small torque on the device which caused its rotation in both configurations. The runs performed with and without electromagnetic shielding covering the capacitor, more or less insulation covering the elements of setup, several changes in the space separation between those elements and the small magnitude of the monitored current in the capacitor indicates that ion wind, electrostatic interaction and also the interaction with the Earth's magnetic field via leakage current cannot explain the magnitude of angular velocity or rotation effect. We hope that the data of our experiments can contribute for enhancement of understanding of the old puzzle.
On the interaction of metric trapping and a boundary
Authors: Datchev Kiril , Metcalfe Jason , Shapiro Jacob , Tohaneanu Mihai
Published: January 2021

By considering a two ended warped product manifold, we demonstrate a bifurcation that can occur when metric trapping interacts with a boundary. In this highly symmetric example, as the boundary passes through the trapped set, one goes from a nontrapping scenario where lossless local energy estimates are available for the wave equation to the case of stably trapped rays where all but a logarithmic amount of decay is lost.
Harmonic currents directed by foliations by Riemann surfaces
Authors: Dinh Tien-Cuong , Wu Hao
Published: January 2021

We study local positive

d

d

c

dd^{c}

-closed currents directed by a foliation by Riemann surfaces near a hyperbolic singularity which have no mass on the separatrices. A theorem of Nguyên says that the Lelong number of such a current at the singular point vanishes. We prove that this property is sharp: one cannot have any better mass estimate for this current near the singularity.
Implementation of a formal pharmacy residency research certificate program
Authors: Darko William , Seabury Robert W , Miller Christopher D , Spinler Sarah A , Probst Luke A , Cleary Lynn M , Kelly Courtney , Kufel Wesley D
Published: January 2021

Purpose

We describe the structure, implementation, and initial evaluation of a formal residency research certificate program (RRCP) designed to further advance residents’ knowledge and skills in research in an effort to better prepare residents for research involvement during their careers.

Pharmacy residency programs vary in the degree of emphasis on research education and training and the structure of resident research activities. Limited data describing formal research education and training for pharmacy residents are available. To better educate and prepare residents in the research process, State University of New York Upstate University Hospital developed and implemented a formal RRCP designed to educate and train residents in essential areas of the research process. Research seminars are delivered by preceptors with experience and training in research throughout the academic year to align with residency project tasks. Residents are also required to complete at least 1 residency project and submit a manuscript suitable for publication in a peer-reviewed journal. Upon successful completion of the program and project requirements, residents earn a certificate of completion. Initial data collected through formal resident assessments before and after RRCP completion demonstrated significant improvement in research knowledge (from an average score of 61.3% out of 100% to an average score of 84.7%, P = 0.002).

Conclusion

Post-RRCP assessment showed improvements in residents’ confidence in several areas of research, including but not limited to research project design, ethical and regulatory principles of research, data collection, selection of appropriate statistical tests, manuscript writing, and the publication process. Residents strongly agreed that the RRCP improved their overall knowledge and perceptions of research.
Standards for Surgical Respirators and Masks: Relevance for Protecting Healthcare Workers and the Public During Pandemics
Authors: Jones Rachael M , Rempel David
Published: January 2021

National standards for surgical respirators and masks are written and enforced to protect healthcare workers from particles and microorganisms such as Severe Acute Respriatory Syndrome Coronavirus 2 (SARS-CoV-2). In addition to the ability to filter particles (e.g. filtration efficiency, FE), the standards address breathability (e.g. differential pressure), how well the mask seals to a worker’s face (e.g. fit test), the level of protection from a fluid splash, and other factors. Standards used in the USA, European Union (EU), and China were compared with respect to testing methods and certification criteria. Although there are substantial similarities in standards for respirators, such as surgical N95, FFP2, and KN95 filtering facepiece respirators (FFRs), there are differences with respect to who performs that testing and fit-testing requirements that influence certification. There is greater variation in test methods between countries for surgical (USA) or medical (EU and China) masks than for FFRs. Surgical/medical masks can be certified to different levels of protection. The impact of the similarities and differences in testing methods and certification criteria on FFR and mask performance for protecting healthcare workers from SARS-CoV-2 are discussed, as well as the value of a new standard in the EU for testing fabrics for masks used by the public. Health and safety personnel in healthcare settings must understand the differences between standards so that they can select respirators and masks that provide appropriate protection for healthcare workers.
The leaf economics spectrum’s morning coffee: plant size-dependent changes in leaf traits and reproductive onset in a perennial tree crop
Authors: Martin Adam R , Isaac Marney E
Published: January 2021

Background and Aims

Size-dependent changes in plant traits are an important source of intraspecific trait variation. However, there are few studies that have tested if leaf trait co-variation and/or trade-offs follow a within-genotype leaf economics spectrum (LES) related to plant size and reproductive onset. To our knowledge, there are no studies on any plant species that have tested whether or not the shape of a within-genotype LES that describes how traits covary across whole plant sizes, is the same as the shape of a within-genotype LES that represents environmentally driven trait plasticity.

Methods

We quantified size-dependent variation in eight leaf traits in a single coffee genotype (Coffea arabica var. Caturra) in managed agroecosystems with different environmental conditions (light and fertilization treatments), and evaluated these patterns with respect to reproductive onset. We also evaluated if trait covariation along a within-genotype plant-size LES differed from a within-genotype environmental LES defined with trait data from coffee growing in different environmental conditions.

Key Results

Leaf economics traits related to resource acquisition – maximum photosynthetic rates (A) and mass-based leaf nitrogen (N) concentrations – declined linearly with plant size. Structural traits – leaf mass, leaf thickness, and leaf mass per unit area (LMA) – and leaf area increased with plant size beyond reproductive onset, then declined in larger plants. Three primary LES traits (mass-based A, leaf N and LMA) covaried across a within-genotype plant-size LES, with plants moving towards the ‘resource-conserving’ end of the LES as they grow larger; in coffee these patterns were nearly identical to a within-genotype environmental LES.

Conclusions

Our results demonstrate that a plant-size LES exists within a single genotype. Our findings indicate that in managed agroecosystems where resource availability is high the role of reproductive onset in driving within-genotype trait variability, and the strength of covariation and trade-offs among LES traits, are less pronounced compared with plants in natural systems. The consistency in trait covariation in coffee along both plant-size and environmental LES axes indicates strong constraints on leaf form and function that exist within plant genotypes.
Discrimination of a single nucleotide polymorphism in the haptoglobin promoter region, rs5472, using a competitive fluorophore-labeled probe hybridization assay following loop-mediated isothermal amplification
Authors: Michiyuki Satoru , Tomita Norihiro , Mori Yasuyoshi , Kanda Hidetoshi , Tashiro Kosuke , Notomi Tsugunori
Published: January 2021

Personalized peptide vaccination, which involves activation of the host immune system against cancer cells using personalized peptide vaccines (PPVs), can improve overall survival in multiple cancer types. However, the clinical efficacies of PPVs vary for unknown reasons. Recently, a single nucleotide polymorphism (NG_012651.1:g.4461_5460[4960A>G]) in the haptoglobin promoter region, rs5472, was significantly associated with clinical response of PPV. Therefore, rs5472 is expected to be a predictive biomarker for PPV therapy. Here, we described a single nucleotide discrimination method for rs5472 analysis by combining the loop-mediated isothermal amplification and quenching probe methods. In evaluation of saliva samples, this method showed high concordance with the results of Sanger sequencing (100%, n = 36). Importantly, this method did not require calculation of melting temperature for single nucleotide discrimination and could therefore be carried out on a simple instrument. Accordingly, this method may be more robust and applicable to near-patient testing.
Role of gambogenic acid in regulating PI3K/Akt/NF-kβ signaling pathways in rat model of acute hepatotoxicity
Authors: Ding Zhongyang , Li Ying , Tang Zhangfeng , Song Xiaoyi , Jing Fa , Wu Haotian , Lu Bei
Published: January 2021

The purpose of this study is to investigate the protective effect of gambogenic acid (GA) in acetaminophen (APAP)-induced hepatotoxicity in rat models. GA (10 mg/kg) was administered intraperitoneal (i.p.) to rats for 7 consecutive days followed by APAP (500 mg/kg) single dose (i.p.) on the final day after GA administration. The levels of MDA, GSH, SOD, CAT, GPx, GST, ALP, AST, ALT, proinflammatory cytokines (TNF-α, IL-1β, IL-6), apoptosis markers (caspase-3 and -9, Bax, Bcl-2), 4-hydroxynonenal (4-HNE), and prostaglandin E2 (PGE2) were evaluated. Results exhibited protective effects of GA by inhibiting inflammation, preventing oxidative stress and apoptosis in APAP-induced liver. Histopathological changes caused by APAP were attenuated, protein expressions of phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) were upregulated, and nuclear factor–kappa β (NF-kβ) was downregulated by GA. In summary, GA significantly exerted anti-inflammatory and antiapoptotic effects against APAP-induced hepatotoxicity potentially through regulation of PI3K/Akt and NF-kβ signaling pathways.
Vitamin C affects G0/G1 cell cycle and autophagy by downregulating of cyclin D1 in gastric carcinoma cells
Authors: Ren Chenxia , Wu Cuiling , Yang Changqing , Lian Changhong
Published: January 2021

Vitamin C has re-emerged as a promising anticancer agent. This study attempts to analyze the differential gene expression of profiles GSE11919 to look for some clues, and the most significant cell cycle pathway caused by vitamin C was identified by integrated bioinformatics analysis. Inspired by this, we investigated the effect of vitamin C treatment on gastric carcinoma cells by detection of cell cycle, apoptosis, and autophagy. Vitamin C significantly elevated the percentage of cells at G0/G1 phase, whereas the percentage of S phase cells was decreased. Meanwhile, vitamin C treatment resulted in downregulation of cell cycle-related protein Cyclin D1. We deduced that the downregulation of Cyclin D1 by vitamin C accompanied by significantly increased 5′AMP-activated protein kinase and induced autophagy in MKN45 cells. These results suggest that vitamin C has the antiproliferation effect on gastric carcinoma cells via the regulation of cell cycle and autophagy by Cyclin D1.
Single-use negative-pressure wound therapy versus conventional dressings for closed surgical incisions: systematic literature review and meta-analysis
Authors: Saunders C , Nherera L M , Horner A , Trueman P
Published: January 2021

Background

Surgical-site complications (SSCs) remain a significant cause of morbidity and mortality, particularly in high-risk patients. The aim of this study was to determine whether prophylactic use of a specific single-use negative-pressure wound therapy (sNPWT) device reduced the incidence of SSCs after closed surgical incisions compared with conventional dressings.

Methods

A systematic literature review was performed using MEDLINE, Embase and the Cochrane Library to identify articles published from January 2011 to August 2018. RCTs and observational studies comparing PICO™ sNPWT with conventional dressings, with at least 10 patients in each treatment arm, were included. Meta-analyses were performed to determine odds ratios (ORs) or mean differences (MDs), as appropriate. PRISMA guidelines were followed. The primary outcome was surgical-site infection (SSI). Secondary outcomes were other SSCs and hospital efficiencies. Risk of bias was assessed.

Results

Of 6197 citations screened, 29 studies enrolling 5614 patients were included in the review; all studies included patients with risk factors for SSCs. sNPWT reduced the number of SSIs (OR 0.37, 95 per cent c.i. 0.28 to 0.50; number needed to treat (NNT) 20). sNPWT reduced the odds of wound dehiscence (OR 0.70, 0.53 to 0.92; NNT 26), seroma (OR 0.23, 0.11 to 0.45; NNT 13) and necrosis (OR 0.11, 0.03 to 0.39; NNT 12). Mean length of hospital stay was shorter in patients who underwent sNPWT (MD −1.75, 95 per cent c.i. −2.69 to −0.81).

Conclusion

Use of the sNPWT device in patients with risk factors reduced the incidence of SSCs and the mean length of hospital stay.
Electronic collection of patient-reported outcomes following discharge after surgery: systematic review
Authors: Tsang C , Lee K S , Richards H , Blazeby J M , Avery K N L
Published: January 2021

Background

Little is known about the electronic collection and clinical feedback of patient-reported outcomes (ePROs) following surgical discharge. This systematic review summarized the evidence on the collection and uses of electronic systems to collect PROs after discharge from hospital after surgery.

Method

Systematic searches of MEDLINE, Embase, PsycINFO, CINAHL and Cochrane Central were undertaken from database inception to July 2019 using terms for ‘patient reported outcomes’, ‘electronic’, ‘surgery’ and ‘at home’. Primary research of all study designs was included if they used electronic systems to collect PRO data in adults after hospital discharge following surgery. Data were collected on the settings, patient groups and specialties, ePRO systems (including features and functions), PRO data collected, and integration with health records.

Results

Fourteen studies were included from 9474 records, including two RCTs and six orthopaedic surgery studies. Most studies (9 of 14) used commercial ePRO systems. Six reported types of electronic device were used: tablets or other portable devices (3 studies), smartphones (2), combination of smartphones, tablets, portable devices and computers (1). Systems had limited features and functions such as real-time clinical feedback (6 studies) and messaging service for patients with care teams (3). No study described ePRO system integration with electronic health records to support clinical feedback.

Conclusion

There is limited reporting of ePRO systems in the surgical literature, and ePRO systems lack integration with hospital clinical systems. Future research should describe the ePRO system and ePRO questionnaires used, and challenges encountered during the study, to support efficient upscaling of ePRO systems using tried and tested approaches.
Safety and feasibility of laparoscopic surgery for elderly rectal cancer patients in Japan: a nationwide study
Authors: Seishima R , Miyata H , Okabayashi K , Hasegawa H , Tsuruta M , Shigeta K , Monno M , Yamashita Y , Inomata M , Wakabayashi G , Kakeji Y , Kitagawa Y , Watanabe M
Published: January 2021

Background

This study aimed to analyse the perioperative results from a national dataset of rectal cancer resections in elderly patients.

Methods

The clinical records of patients undergoing rectal cancer surgery between 2012 and 2014 were retrieved from the Japanese National Clinical Database and analysed retrospectively. Patients were categorized according to age and those 80 years or older were defined as elderly. Subgroups were also defined according to the surgical approach (laparoscopy versus open surgery). The short-term outcomes, including mortality, anastomotic leak, surgical site infections and medical complications were compared between subgroups.

Results

Of 56 175 patients undergoing rectal cancer surgery, some 6717 patients were elderly and laparoscopy was performed in 46.8 per cent of the sample. When comparing laparoscopy and open surgery in elderly patients, the operative mortality rate (1.5 versus 2.8 per cent; P < 0.001), the incidence of anastomotic leakage (5.2 versus 6.5 per cent; P = 0.026), surgical site infections (6.0 versus 8.0 per cent; P = 0.001), pneumonia (1.4 versus 2.5 per cent; P = 0.001), renal failure (0.7 versus 1.3 per cent; P = 0.016) and cardiac events (0.3 versus 0.8 per cent; P = 0.008) were lower for laparoscopy than for open surgery. The overall complication rate in elderly patients (19.5 per cent) was comparable to that in the younger group (P = 0.07). However, incidence of systemic complications was significantly higher in elderly than in younger patients (all P < 0.001).

Conclusion

Laparoscopy was safe and feasible in elderly patients compared with open surgery. However, the rates of systemic complications were significantly higher than in younger patients.
‘A World to Win’: In Defence of (Dissenting) Social Work—A Response to Chris Maylea
Authors: Michael Garrett Paul
Published: January 2021

This article is a response to Chris Maylea’s lively call to ‘end of social work’. Whilst welcoming the publication of his polemic, I maintain that there are substantial problems with his lines of analysis. Problematic facets of his article are enmeshed with questions of timing, tonality and positionality. It is also possible to identify significant evidential flaws in the bald assertion that social workers present a ‘real threat to people’. What is more, Maylea’s inadvertently risks aiding the project of the neoliberal Right. Critically engaging with the notion that there are ‘four key reasons’ necessitating the abolition of profession, I make an alternative case for reanimated and re-energised forms of ‘dissenting social work’.
Decoding Neural Representations of Affective Scenes in Retinotopic Visual Cortex
Authors: Bo Ke , Yin Siyang , Liu Yuelu , Hu Zhenhong , Meyyappan Sreenivasan , Kim Sungkean , Keil Andreas , Ding Mingzhou
Published: January 2021

The perception of opportunities and threats in complex visual scenes represents one of the main functions of the human visual system. The underlying neurophysiology is often studied by having observers view pictures varying in affective content. It has been shown that viewing emotionally engaging, compared with neutral, pictures (1) heightens blood flow in limbic, frontoparietal, and anterior visual structures and (2) enhances the late positive event-related potential (LPP). The role of retinotopic visual cortex in this process has, however, been contentious, with competing theories predicting the presence versus absence of emotion-specific signals in retinotopic visual areas. Recording simultaneous electroencephalography–functional magnetic resonance imaging while observers viewed pleasant, unpleasant, and neutral affective pictures, and applying multivariate pattern analysis, we found that (1) unpleasant versus neutral and pleasant versus neutral decoding accuracy were well above chance level in retinotopic visual areas, (2) decoding accuracy in ventral visual cortex (VVC), but not in early or dorsal visual cortex, was correlated with LPP, and (3) effective connectivity from amygdala to VVC predicted unpleasant versus neutral decoding accuracy, whereas effective connectivity from ventral frontal cortex to VVC predicted pleasant versus neutral decoding accuracy. These results suggest that affective scenes evoke valence-specific neural representations in retinotopic visual cortex and that these representations are influenced by reentry signals from anterior brain regions.
Evaluation of Severe Acute Respiratory Syndrome Coronavirus 2 Transmission Mitigation Strategies on a University Campus Using an Agent-Based Network Model
Authors: Goyal Ravi , Hotchkiss John , Schooley Robert T , De Gruttola Victor , Martin Natasha K
Published: January 2021

Universities are faced with decisions on how to resume campus activities while mitigating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) risk. To provide guidance for these decisions, we developed an agent-based network model of SARS-CoV-2 transmission to assess the potential impact of strategies to reduce outbreaks. The model incorporates important features related to risk at the University of California San Diego. We found that structural interventions for housing (singles only) and instructional changes (from in-person to hybrid with class size caps) can substantially reduce the basic reproduction number, but masking and social distancing are required to reduce this to at or below 1. Within a risk mitigation scenario, increased frequency of asymptomatic testing from monthly to twice weekly has minimal impact on average outbreak size (1.1–1.9), but substantially reduces the maximum outbreak size and cumulative number of cases. We conclude that an interdependent approach incorporating risk mitigation, viral detection, and public health intervention is required to mitigate risk.
Olfactory Cues From Host- and Non-host Plant Odor Influence the Behavioral Responses of AdultDrosophila suzukii(Diptera: Drosophilidae) to Visual Cues
Authors: Bolton L Grant , Piñero Jaime C , Barrett Bruce A
Published: January 2021

While trapping methods for Drosophila suzukii Matsumura (Diptera: Drosophilidae) have typically relied on fermentation volatiles alone or in association with a visual stimulus, the relative contribution of visual and olfactory stimuli to the food- and host-seeking behavior of D. suzukii is poorly understood. This study quantified the type of response exhibited by male and female D. suzukii to color and the effects that volatiles (fermentation, fresh fruit, and leaf) exert on the outcome. Seven-, four- and two-choice assays were used to quantify interactions between visual and olfactory cues. When no volatiles were present in a seven-choice assay, D. suzukii preferred red, black, and green pigments. Black and red were preferred when yeast odors were present, and black alone was the most attractive color when blueberry odor was present. A strawberry leaf terpenoid, β-cyclocitral, seemed to have overridden the flies’ response to color. In four-choice assays, blueberry odor was more likely to interact synergistically with color than yeast or β-cyclocitral. This study demonstrates that D. suzukii modulates the response to multimodal sensory modalities (vision and olfaction) depending, to some extent, on the type of olfactory stimuli. Our findings also provide insight into the relative importance of vision as a function of odor quality in this invasive species.
Electrocardiogram machine learning for detection of cardiovascular disease in African Americans: the Jackson Heart Study
Authors: Pollard James D , Haq Kazi T , Lutz Katherine J , Rogovoy Nichole M , Paternostro Kevin A , Soliman Elsayed Z , Maher Joseph , Lima João A C , Musani Solomon K , Tereshchenko Larisa G
Published: January 2021

Aims

Almost half of African American (AA) men and women have cardiovascular disease (CVD). Detection of prevalent CVD in community settings would facilitate secondary prevention of CVD. We sought to develop a tool for automated CVD detection.

Methods and results

Participants from the Jackson Heart Study (JHS) with analysable electrocardiograms (ECGs) (n = 3679; age, 62 ± 12 years; 36% men) were included. Vectorcardiographic (VCG) metrics QRS, T, and spatial ventricular gradient vectors’ magnitude and direction, and traditional ECG metrics were measured on 12-lead ECG. Random forests, convolutional neural network (CNN), lasso, adaptive lasso, plugin lasso, elastic net, ridge, and logistic regression models were developed in 80% and validated in 20% samples. We compared models with demographic, clinical, and VCG input (43 predictors) and those after the addition of ECG metrics (695 predictors). Prevalent CVD was diagnosed in 411 out of 3679 participants (11.2%). Machine learning models detected CVD with the area under the receiver operator curve (ROC AUC) 0.69–0.74. There was no difference in CVD detection accuracy between models with VCG and VCG + ECG input. Models with VCG input were better calibrated than models with ECG input. Plugin-based lasso model consisting of only two predictors (age and peak QRS-T angle) detected CVD with AUC 0.687 [95% confidence interval (CI) 0.625–0.749], which was similar (P = 0.394) to the CNN (0.660; 95% CI 0.597–0.722) and better (P < 0.0001) than random forests (0.512; 95% CI 0.493–0.530).

Conclusions

Simple model (age and QRS-T angle) can be used for prevalent CVD detection in limited-resources community settings, which opens an avenue for secondary prevention of CVD in underserved communities.
Temporary Relief of Pay-for-Delay: The ECJ as Specifically Different Antidepressant
Authors: Gschwindt Daniela
Published: January 2021

Pay-for-delay agreements are increasingly scrutinised and sanctioned in the European Union (EU). The state of play so far has only made it possible to analyse such agreements as restrictions of competition by object under Art. 101(1) of the Treaty on the Functioning of the European Union (TFEU) and in some cases as abuses of a dominant position under Art. 102 TFEU. At the beginning of this year, the European Court of Justice (ECJ) gave its first ruling on this issue. The Generics judgment (C-307/18) deepens the reasoning and defined line of the General Court (EGC) and the Commission and deals with fundamental questions of competition law. To this end, the Court has extended the existing investigation criteria. Although the judgment consolidates the status of pay-for-delay, it fails to resolve important aspects and leaves only losers. This comment takes the ECJ’s decision in Generics as an opportunity to analyse pay-for-delay agreements in the EU from a critical perspective.
Cutting out the camel-like knees of St. James: the de viris illustribus tradition in the twelfth-century renaissance
Authors: Byrne Philippa
Published: January 2021

This article examines the revival of the de viris illustribus genre in the twelfth century. These catalogues of the most important Christian authors were modelled on a template created in late antiquity by Jerome, but adopted new attitudes to what constitutes distinguished Christian literary activity. They regard the editing, collecting, and re-ordering of texts as equal to the composition of new works, and recognize legal codification as a noteworthy activity. As such, they represent the broadening out of a Latin Christian literary canon. These texts complicate our ideas about the relationship between ‘authority’ and ‘amplification’ in the twelfth-century renaissance.
Evaluation of a novel animal milk oligosaccharide biosimilar: macronutrient digestibility and gastrointestinal tolerance, fecal metabolites, and fecal microbiota of healthy adult dogs and in vitro genotoxicity assays
Authors: Lee Anne H , Vidal Sara , Oba Patrícia M , Wyss Romain , Miao Yong , Adesokan Yemi , Swanson Kelly S
Published: January 2021

Milk oligosaccharides (MO) are bioactive compounds in mammalian milk that provide health benefits to neonates beyond essential nutrients. GNU100, a novel animal MO biosimilar, was recently tested in vitro, with results showing beneficial shifts in microbiota and increased short-chain fatty acid (SCFA) production, but other effects of GNU100 were unknown. Three studies were conducted to evaluate the safety, palatability, and gastrointestinal (GI) tolerance of GNU100. In study 1, the mutagenic potential of GNU100 was tested using a bacterial reverse mutation assay and a mammalian cell micronucleus test. In study 2, palatability was assessed by comparing diets containing 0% vs. 1% GNU100 in 20 adult dogs. In study 3, 32 adult dogs were used in a completely randomized design to assess the safety and GI tolerance of GNU100 and explore utility. Following a 2-wk baseline, dogs were assigned to one of four treatments and fed for 26 wk: 0%, 0.5%, 1%, and 1.5% GNU100. On weeks 2, 4, and 26, fresh fecal samples were collected to measure stool quality, immunoglobulin A, and calprotectin, and blood samples were collected to measure serum chemistry, inflammatory markers, and hematology. On weeks 2 and 4, fresh fecal samples were collected to measure metabolites and microbiota. On week 4, total feces were collected to assess apparent total tract macronutrient digestibility. Although revertant numbers were greater compared with the solvent control in tester strain WP2uvrA(pKM101) in the presence of metabolic activation (S9) in the initial experiment, they remained below the threshold for a positive mutagenic response in follow-up confirmatory tests, supporting that GNU100 is not mutagenic. Similarly, no cytotoxicity or chromosome damage was observed in the cell micronucleus test. The palatability test showed that 1% GNU100 was strongly preferred (P < 0.05; 3.6:1 consumption ratio) over the control. In study 3, all dogs were healthy and had no signs of GI intolerance or illness. All diets were well accepted, and food intake, fecal characteristics, metabolite concentrations, and macronutrient digestibilities were not altered. GNU100 modulated fecal microbiota, increasing evenness and Catenibacterium, Megamonas, and Prevotella (SCFA producers) and reducing Collinsella. Overall, the results suggest that GNU100 is palatable and well-tolerated, causes no genotoxicity or adverse effects on health, and beneficially shifts the fecal microbiota, supporting the safety of GNU100 for the inclusion in canine diets.
Synthetic utility of oxygenases in site-selective terpenoid functionalization
Authors: Renata Hans
Published: January 2021

Terpenoids are one of the largest classes of natural products whose members possess a wide variety of biological activities. With several exceptions, scalable production of complex terpenoids with either purely biological or chemical methods still remains a major challenge. However, recent efforts to combine the two approaches in chemoenzymatic synthesis hold tremendous promise to address this challenge. Central to this paradigm is the development of useful biocatalytic methods, such as regioselective C–H oxidation, for terpene modifications. This review highlights recent applications of biocatalytic hydroxylation for site-selective modification of terpenoids.
Soybean Gall Midge (Diptera: Cecidomyiidae), a New Species Causing Injury to Soybean in the United States
Authors: McMechan Anthony J , Hodgson Erin W , Varenhorst Adam J , Hunt Thomas , Wright Robert , Potter Bruce
Published: January 2021

The soybean gall midge (Resseliella maxima Gagné) was recently identified as a new species causing injury to soybean in the Midwestern United States. Although this insect was only recently identified, it has likely been present in soybean fields for at least the last 8 yr based on anecdotal reports. The soybean gall midge has historically been observed late in the season on soybean plants that were believed to have been previously compromised by a plant pathogen or mechanical damage with little to no concern for economic losses. In late June 2018, dead and dying plants were found to be associated with the soybean gall midge across four midwestern states. The distribution of plant injury in the field, larval feeding within the stem, and timing of infestation indicate that it is likely an important pest of soybean. Yield losses in soybean gall midge infested fields can be up to 100% for the first 30 meters from the field edge, with losses of 17–31% further into the field. The rapid development of the soybean gall midge as an important pest of soybean has left large gaps in the knowledge necessary to develop an integrated pest management program.
Reinertia, a New Subgenus of the Genus Aedes Meigen and Its Type Species Aedes (Reinertia) suffusus (Diptera: Culicidae), Newly Recorded From Bhutan
Authors: Somboon Pradya , Phanitchakun Thanari , Saingamsook Jassada , Namgay Rinzin , Harbach Ralph E
Published: January 2021

A new subgenus, Reinertia Somboon, Namgay & Harbach, of the genus Aedes Meigen and its type species, Ae. suffusus Edwards, are described from specimens reared from larvae and pupae found in a tree hole in Bhutan. The scutum of the adults is mostly covered with narrow pale falcate scales. The proboscis, maxillary palpus, tibiae, and tarsi are dark-scaled. The gonocoxite of the male genitalia bears a unique setose basomesal sclerite. The larva closely resembles larvae of the subgenus Downsiomyia Vargus in having setae 4–6-C with numerous branches and inserted more or less on level with seta 7-C, abdominal seta 12-I is present and the comb is composed of 6–10 spine-like scales arranged in an irregular row. Surprisingly, Reinertia shares features of the adult habitus, male genitalia, and larva with the Palearctic subgenus Dahliana Reinert, Harbach & Kitching. However, in phylogenetic analyses of the mitochondrial COI gene of species representing 38 subgenera of Aedes and six other genera of the tribe Aedini Neveu-Lemaire, Reinertia was not associated with Dahliana or Downsiomyia. In both maximum likelihood and Bayesian analyses of the data, Ae. suffusus was recovered as the weakly supported sister of a clade composed of five species of the subgenus Protomacleaya Theobald. In the absence of strong support, and because Protomacleaya is an unnatural group of species that resemble each other phenetically by virtue of what they lack, Ae. suffusus cannot be placed in the subgenus Protomacleaya. Thus, the morphological and molecular data attest the uniqueness of Ae. suffusus and its recognition as a monobasic subgeneric lineage.
Abundance of Ticks (Acari: Ixodidae) and Presence ofRickettsiaandAnaplasmain Ticks Infesting Domestic Animals From Northern India
Authors: Misra Brij Ranjan , Kumar Niraj , Kant Rajni , Deval Hirawati , Singh Rajeev , Pandey Ashok Kumar , Behera Sthita Pragnya , Bondre Vijay P
Published: January 2021

Rickettsia and Anaplasma are bacteria that can be transmitted by hematophagous arthropods such as ticks infesting animals in close proximity to humans. The main objective of the present study was to investigate abundance of common tick species infesting domestic animals and presence of Rickettsia and Anaplasma in tick populations. Adult ticks were collected from domestic animals in rural areas and screened by molecular detection of bacterial DNA for these two genera of bacteria. A total of 1,778 adult ixodid tick specimens were collected from 200 cattle, 200 buffaloes, 200 goats, and 40 dogs. The collection consisted of four species of ixodid ticks, Rhipicephalus (Boophilus) microplus (Canestrini) (83.8%), Hyalomma kumari (Sharif) (7.1%), Rhipicephalus sanguineus (Latreille) (6.4%), and Dermacentor auratus (Supino) (2.7%) infesting the domestic animals. The prevalence of all the collected tick species was highest in the month of October. Anaplasma spp. was the most frequently identified bacteria (3.3%) in tested ticks. Of 17 positive tick pools for Anaplasma spp., 14 pools were from ticks infesting cattle, 2 pools of ticks collected from buffalo, and the remaining pool were ticks infesting a goat at the time of collection. Although 1.6% tick pools of R. microplus collected from cattle tested positive for Rickettsia spp., present investigation provides evidence of the most prevalent ixodid ticks infesting domestic animals and the presence of obligate intracellular bacteria, Rickettsia and Anaplasma, in these ticks collected in the Gorakhpur division of Northern India.
Teicoplanin Therapeutic Drug Monitoring and Treatment Outcomes in Children With Gram-Positive Infections
Authors: Loane Georgia , Gwee Amanda
Published: January 2021

This is a retrospective audit of teicoplanin dosing, drug-level monitoring, and outcomes in 29 children who received 31 courses of teicoplanin. Our study of noncritically ill children showed that the majority (35/43, 81%) of trough concentrations were within the therapeutic range (10-30 mg/L) contrasting previous studies in children. Overall, 21 of the 24 (88%) children with Gram-positive infections achieved cure.
Phytosulfokine (PSK) precursor processing by subtilase SBT3.8 and PSK signaling improve drought stress tolerance in Arabidopsis
Authors: Stührwohldt Nils , Bühler Eric , Sauter Margret , Schaller Andreas
Published: January 2021

Increasing drought stress poses a severe threat to agricultural productivity. Plants, however, have evolved numerous mechanisms to cope with such environmental stress. Here we report that the stress-induced production of a peptide signal contributes to stress tolerance. The expression of phytosulfokine (PSK) peptide precursor genes, and transcripts of three subtilisin-like serine proteases, SBT1.4, SBT3.7, and SBT3.8, were found to be up-regulated in response to osmotic stress. Stress symptoms were more pronounced in sbt3.8 loss-of-function mutants and could be alleviated by PSK treatment. Osmotic stress tolerance was improved in plants overexpressing the PSK1 precursor (proPSK1) or SBT3.8, resulting in higher fresh weight and improved lateral root development in transgenic plants compared with wild-type plants. We further showed that SBT3.8 is involved in the biogenesis of the bioactive PSK peptide. ProPSK1 was cleaved by SBT3.8 at the C-terminus of the PSK pentapeptide. Processing by SBT3.8 depended on the aspartic acid residue directly following the cleavage site. ProPSK1 processing was impaired in the sbt3.8 mutant. The data suggest that increased expression of proPSK1 in response to osmotic stress followed by the post-translational processing of proPSK1 by SBT3.8 leads to the production of PSK as a peptide signal for stress mitigation.
The massive M31 cluster G1: detailed chemical abundances from integrated light spectroscopy★
Authors: Sakari Charli M , Shetrone Matthew D , McWilliam Andrew , Wallerstein George
Published: January 2021

G1, also known as Mayall II, is one of the most massive star clusters in M31. Its mass, ellipticity, and location in the outer halo make it a compelling candidate for a former nuclear star cluster. This paper presents an integrated light abundance analysis of G1, based on a moderately high-resolution (R = 15 000) spectrum obtained with the high-resolution spectrograph on the Hobby–Eberly Telescope in 2007 and 2008. To independently determine the metallicity, a moderate-resolution (R ∼ 4000) spectrum of the Ca ii triplet lines in the near-infrared was also obtained with the Astrophysical Research Consortium’s 3.5-m telescope at Apache Point Observatory. From the high-resolution spectrum, G1 is found to be a moderately metal-poor cluster, with $[\rm {Fe/H}]~=~-0.98\pm 0.05$. G1 also shows signs of α-enhancement (based on Mg, Ca, and Ti) and lacks the s-process enhancements seen in dwarf galaxies (based on comparisons of Y, Ba, and Eu), indicating that it originated in a fairly massive galaxy. Intriguingly, G1 also exhibits signs of Na and Al enhancement, a unique signature of GCs – which suggests that G1’s formation is intimately connected with GC formation. G1’s high [Na/Fe] also extends previous trends with cluster velocity dispersion to an even higher mass regime, implying that higher mass clusters are more able to retain Na-enhanced ejecta. The effects of intracluster abundance spreads are discussed in a subsequent paper. Ultimately, G1’s chemical properties are found to resemble other M31 GCs, though it also shares some similarities with extragalactic nuclear star clusters.
Robot-Assisted Therapy for Upper Extremity Motor Impairment After Stroke: A Systematic Review and Meta-Analysis
Authors: Wu Jingyi , Cheng Hao , Zhang Jiaqi , Yang Shanli , Cai Sufang
Published: January 2021

Objective

The purpose of this study was to review the effects of robot-assisted therapy (RT) for improving poststroke upper extremity motor impairment.

Methods

The PubMed, Embase, Medline, and Web of Science databases were searched from inception to April 8, 2020. Randomized controlled trials that were conducted to evaluate the effects of RT on upper extremity motor impairment poststroke and that used Fugl-Meyer assessment for upper extremity scores as an outcome were included. Two authors independently screened articles, extracted data, and assessed the methodological quality of the included studies using the Physiotherapy Evidence Database (PEDro) scale. A random-effects meta-analysis was performed to pool the effect sizes across the studies.

Results

Forty-one randomized controlled trials with 1916 stroke patients were included. Compared with dose-matched conventional rehabilitation, RT significantly improved the Fugl-Meyer assessment for upper extremity scores of the patients with stroke, with a small effect size (Hedges g = 0.25; 95% CI, 0.11-0.38; I2 = 45.9%). The subgroup analysis revealed that the effects of unilateral RT, but not that of bilateral RT, were superior to conventional rehabilitation (Hedges g = 0.32; 95% CI, 0.15-0.50; I2 = 55.9%). Regarding the type of robot devices, the effects of the end effector device (Hedges g = 0.22; 95% CI, 0.09–0.36; I2 = 35.4%), but not the exoskeleton device, were superior to conventional rehabilitation. Regarding the stroke stage, the between-group difference (ie, RT vs convention rehabilitation) was significant only for people with late subacute or chronic stroke (Hedges g = 0.33; 95% CI, 0.16-0.50; I2 = 34.2%).

Conclusion

RT might be superior to conventional rehabilitation in improving upper extremity motor impairment in people after stroke with notable upper extremity hemiplegia and limited potential for spontaneous recovery.
Complex differences in infection rates between ethnic groups in Scotland: a retrospective, national census-linked cohort study of 1.65 million cases
Authors: Gruer L D , Cézard G I , Wallace L A , Hutchinson S J , Douglas A F , Buchanan D , Katikireddi S V , Millard A D , Goldberg D J , Sheikh A , Bhopal R S
Published: January 2021

Background

Ethnicity can influence susceptibility to infection, as COVID-19 has shown. Few countries have systematically investigated ethnic variations in infection.

Methods

We linked the Scotland 2001 Census, including ethnic group, to national databases of hospitalizations/deaths and serological diagnoses of bloodborne viruses for 2001–2013. We calculated age-adjusted rate ratios (RRs) in 12 ethnic groups for all infections combined, 15 infection categories, and human immunodeficiency virus (HIV), hepatitis B (HBV) and hepatitis C (HCV) viruses.

Results

We analysed over 1.65 million infection-related hospitalisations/deaths. Compared with White Scottish, RRs for all infections combined were 0.8 or lower for Other White British, Other White and Chinese males and females, and 1.2–1.4 for Pakistani and African males and females. Adjustment for socioeconomic status or birthplace had little effect. RRs for specific infection categories followed similar patterns with striking exceptions. For HIV, RRs were 136 in African females and 14 in males; for HBV, 125 in Chinese females and 59 in males, 55 in African females and 24 in males; and for HCV, 2.3–3.1 in Pakistanis and Africans.

Conclusions

Ethnic differences were found in overall rates and many infection categories, suggesting multiple causative pathways. We recommend census linkage as a powerful method for studying the disproportionate impact of COVID-19.
Anayudato the least advantaged: providing a program for those who were hit the hardest during the COVID-19 pandemic
Authors: Gozum Ivan Efreaim A , Capulong Harvey Gain M , Galang Joseph Renus F , Gopez Jose Ma W
Published: January 2021

In a recent article, it was reported that the COVID-19 pandemic impacted the economic situation of some countries. It led to some people losing jobs amidst the difficult situation that the pandemic caused. Thus, this paper discusses the need to provide a program for the least advantaged citizens so that they can still experience their basic liberties. The least advantaged citizens, as Rawls deem them, are the ones who lost jobs and incomes were hit the hardest during the COVID-19 pandemic.
Forecasting dynamic body weight of nonrestrained pigs from images using an RGB-D sensor camera
Authors: Yu Haipeng , Lee Kiho , Morota Gota
Published: January 2021

Average daily gain is an indicator of the growth rate, feed efficiency, and current health status of livestock species including pigs. Continuous monitoring of daily gain in pigs aids producers to optimize their growth performance while ensuring animal welfare and sustainability, such as reducing stress reactions and feed waste. Computer vision has been used to predict live body weight from video images without direct handling of the pig. In most studies, videos were taken while pigs were immobilized at a weighing station or feeding area to facilitate data collection. An alternative approach is to capture videos while pigs are allowed to move freely within their own housing environment, which can be easily applied to the production system as no special imaging station needs to be established. The objective of this study was to establish a computer vision system by collecting RGB-D videos to capture top-view red, green, and blue (RGB) and depth images of nonrestrained, growing pigs to predict their body weight over time. Over a period of 38 d, eight growers were video recorded for approximately 3 min/d, at the rate of six frames per second, and manually weighed using an electronic scale. An image-processing pipeline in Python using OpenCV was developed to process the images. Specifically, each pig within the RGB frame was segmented by a thresholding algorithm, and the contour of the pig was identified to extract its length and width. The height of a pig was estimated from the depth images captured by the infrared depth sensor. Quality control included removing pigs that were touching the fence and sitting, as well as those showing extremely distorted shape or motion blur owing to their frequent movement. Fitting all of the morphological image descriptors simultaneously in linear mixed models yielded prediction coefficients of determination of 0.72–0.98, 0.65–0.95, 0.51–0.94, and 0.49–0.93 for 1-, 2-, 3-, and 4-d ahead forecasting, respectively, of body weight in time series cross-validation. Based on the results, we conclude that our RGB-D sensor-based imaging system coupled with the Python image-processing pipeline could potentially provide an effective approach to predict the live body weight of nonrestrained pigs from images.