bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of leaf rust resistance loci in a geographically diverse panel of wheat using genome-wide association analysis
Authors: Kaur Shivreet , Gill Harsimardeep S. , Breiland Matthew , Kolmer James A. , Gupta Rajeev , Sehgal Sunish K. , Gill Upinder
Published: February 2023

Leaf rust, caused by Puccinia triticina (Pt) is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent Pt races in North America calls for exploring new sources of leaf rust resistance. A diversity panel of 365 bread wheat accessions selected from a worldwide population of landraces and cultivars was evaluated at the seedling stage against four Pt races (TDBJQ, TBBGS, MNPSD and, TNBJS). A wide distribution of seedling responses against the four Pt races was observed. Majority of the genotypes displayed a susceptible response with only 28 (9.8%), 59 (13.5%), 45 (12.5%), and 29 (8.1%) wheat accessions exhibiting a highly resistant response to TDBJQ, TBBGS, MNPSD and, TNBJS, respectively. Further, we conducted a high-resolution multi-locus genome-wide association study (GWAS) using a set of 302,524 high-quality single nucleotide polymorphisms (SNPs). The GWAS analysis identified 27 marker-trait associations (MTAs) for leaf rust resistance on different wheat chromosomes of which 20 MTAs were found in the vicinity of known Lr genes, MTAs, or quantitative traits loci (QTLs) identified in previous studies. The remaining seven significant MTAs identified represent genomic regions that harbor potentially novel genes for leaf rust resistance. Furthermore, the candidate gene analysis for the significant MTAs identified various genes of interest that may be involved in disease resistance. The identified resistant lines and SNPs linked to the QTLs in this study will serve as valuable resources in wheat rust resistance breeding programs.
The evolution and expression of stomatal regulators in C3 and C4 crops: Implications on the divergent drought tolerance
Authors: Song Zhuojun , Wang Le , Lee May , Yue Gen Hua
Published: February 2023

Drought stress is a major environmental hazard. Stomatal development is highly responsive to abiotic stress and has been used as a cellular marker for drought-tolerant crop selection. C3 and C4 crops have evolved into different photosynthetic systems and physiological responses to water deficits. The genome sequences of maize, sorghum, and sugarcane make it possible to explore the association of the stomatal response to drought stress with the evolution of the key stomatal regulators. In this study, phylogenic analysis, gene expression analysis and stomatal assay under drought stress were used to investigate the drought tolerance of C3 and C4 plants. Our data shows that C3 and C4 plants exhibit different drought responses at the cellular level. Drought represses the growth and stomatal development of C3 crops but has little effect on that of C4 plants. In addition, stomatal development is unresponsive to drought in drought-tolerant C3 crops but is repressed in drought-tolerant C4 plants. The different developmental responses to drought in C3 and C4 plants might be associated with the divergent expression of their SPEECHLESS genes. In particular, C4 crops have evolved to generate multiple SPEECHLESS homologs with different genetic structure and expression levels. Our research provides not only molecular evidence that supports the evolutionary history of C4 from C3 plants but also a possible molecular model that controls the cellular response to abiotic stress in C3 and C4 crops.
Challenges of teachers when teaching sentence-based mathematics problem-solving skills
Authors: Ling Albert Nguong Baul , Mahmud Muhammad Sofwan
Published: February 2023

Sentence-based mathematics problem-solving skills are essential as the skills can improve the ability to deal with various mathematical problems in daily life, increase the imagination, develop creativity, and develop an individual’s comprehension skills. However, mastery of these skills among students is still unsatisfactory because students often find it difficult to understand mathematical problems in verse, are weak at planning the correct solution strategy, and often make mistakes in their calculations. This study was conducted to identify the challenges that mathematics teachers face when teaching sentence-based mathematics problem-solving skills and the approaches used to address these challenges. This study was conducted qualitatively in the form of a case study. The data were collected through observations and interviews with two respondents who teach mathematics to year four students in a Chinese national primary school in Kuala Lumpur. This study shows that the teachers have faced three challenges, specifically low mastery skills among the students, insufficient teaching time, and a lack of ICT infrastructure. The teachers addressed these challenges with creativity and enthusiasm to diversify the teaching approaches to face the challenges and develop interest and skills as part of solving sentence-based mathematics problems among year four students. These findings allow mathematics teachers to understand the challenges faced while teaching sentence-based mathematics problem solving in depth as part of delivering quality education for every student. Nevertheless, further studies involving many respondents are needed to understand the problems and challenges of different situations and approaches that can be used when teaching sentence-based mathematics problem-solving skills.
The association between workplace ostracism and knowledge-sharing behaviors among Chinese university teachers: The chain mediating model of job burnout and job satisfaction
Authors: Wang Guang-Hui , Li Jia-Hui , Liu Hui , Zaggia Cristina
Published: February 2023

Workplace ostracism is an essential predictor of knowledge-sharing behaviors, but few studies have explored the influence of this mechanism in higher education. According to the conservation of resources theory, this study elucidated the roles of job burnout and job satisfaction as sequential mediators of the link between workplace ostracism and knowledge-sharing behaviors in a sample of 388 university teachers. The results of the study were analyzed via structural equation modeling (SEM). Higher knowledge-sharing behaviors were associated with lower workplace ostracism, lower job burnout, and more job satisfaction. Furthermore, increased workplace ostracism was associated with more job burnout, but job satisfaction was not related to workplace ostracism. The relationship between workplace ostracism and knowledge-sharing behaviors was mediated by job burnout and was sequentially mediated by job burnout and job satisfaction. These findings help to clarify the mechanisms underlying the association between workplace ostracism and knowledge-sharing behaviors in university teachers. The theoretical and practical implications of the findings are discussed.
A psychometric evaluation of Chinese chronic hepatitis B virus infection-related stigma scale using classical test theory and item response theory
Authors: Zhong Sirui , Zhou Yuxiao , Zhumajiang Wuerken , Feng Lifen , Gu Jing , Lin Xiao , Hao Yuantao
Published: February 2023

Purpose

To validate the hepatitis B virus infection-related stigma scale (HBVISS) using Classical Test Theory and Item Response Theory in a sample of Chinese chronic HBV carriers.

Methods

Feasibility, internal consistency reliability, split-half reliability and construct validity were evaluated using a cross-sectional validation study (n = 1,058) in Classical Test Theory. Content validity was assessed by COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) criteria. The Item Response Theory (IRT) model parameters were estimated using Samejima’s graded response model, after which item response category characteristic curves were drawn. Item information, test information, and IRT-based marginal reliability were calculated. Measurement invariance was assessed using differential item functioning (DIF). SPSS and R software were used for the analysis.

Results

The response rate reached 96.4% and the scale was completed in an average time of 5 min. Content validity of HBVISS was sufficient (+) and the quality of the evidence was high according to COSMIN criteria. Confirmatory factor analysis showed acceptable goodness-of-fit (χ2/df = 5.40, standardized root mean square residual = 0.057, root mean square error of approximation = 0.064, goodness-of-fit index = 0.902, comparative fit index = 0.925, incremental fit index = 0.926, and Tucker-Lewis index = 0.912). Cronbach’s α fell in the range of 0.79–0.89 for each dimension and 0.93 for the total scale. Split-half reliability was 0.96. IRT discrimination parameters were estimated to range between 0.959 and 2.333, and the threshold parameters were in the range-3.767 to 3.894. The average score for test information was 12.75 (information >10) when the theta level reached between-4 and + 4. The IRT-based marginal reliability was 0.95 for the total scale and fell in the range of 0.83–0.91 for each dimension. No measurement invariance was detected (d-R2 < 0.02).

Conclusion

HBVISS exhibited good feasibility, reliability, validity, and item quality, making it suitable for assessing chronic Hepatitis B virus infection-related stigma.
Psychological distress among healthcare providers in oncology during the COVID-19 pandemic in Japan: The mediating role of moral distress and resilience
Authors: Okamura Masako , Fujimori Maiko , Goto Shinichi , Ohisa Keiko , Boku Narikazu , Nakahara Rika , Uchitomi Yosuke , Suzuki Tatsuya , Matsuda Tomohiro
Published: February 2023

Objective

Even though vaccines have become widespread, there is an explosion of infection due to the emergence of new mutant strains, and support for healthcare providers’ mental health is necessary. The aims of this study were to explore factors associated with the psychological distress, and to determine the degree of association between moral distress, resilience and psychological distress in order to consider intervention models for psychological distress of healthcare providers involved with cancer patients during the COVID-19 pandemic.

Method

We conducted a cross-sectional survey among healthcare providers at the National Cancer Center, Japan. Psychological distress was assessed by the Hospital Anxiety and Depression Scale. We also assessed moral distress using the Moral Distress Thermometer and resilience using the Connor-Davidson Resilience Scale 10 in April and May 2020 which was the first surge of the epidemic period.

Results

Five hundred sixty-six of 3,900 healthcare providers (14.5%) responded. Sixty-eight percent (385/566) responders were above the Hospital Anxiety and Depression Scale cutoff. Hierarchical regression analyses indicated that nurses, allied health professionals and office workers/engineers (odds ratio = 4.63; 95% confidence interval 1.90–11.29; p < 0.001, odds ratio = 3.88; 95% confidence interval 1.88–8.00; p < 0.001, odds ratio = 2.10; 95% confidence interval 1.06–4.18; p < 0.05) as well as healthcare providers with low resilience (odds ratio = 0.88; 95% confidence interval 0.85–0.91; p < 0.001) were at risk of psychological distress. Moral distress was not significantly associated with prevalence of psychological distress.

Conclusion

During the first surge of the pandemic, a high prevalence of psychological distress was demonstrated among cancer center healthcare providers. The study suggests that mental health care should be available to cancer care providers. Since the COVID-19 pandemic is still going on, in addition to the efforts by our facilities, we should consider interventions that promote resilience and a feasibility study of these interventions.
Risk perception of COVID-19 and its related factors among centralized medical isolation groups in China
Authors: Zhang Rui , Wang Chengli
Published: February 2023

Objective

Medical isolation is one of the most effective measures to slow the spread of the virus when dealing with a pandemic. Millions of people in China have undergone centralized medical isolation (CMI) during the COVID-19 pandemic. This study aims to assess the centralized medical isolation group’s COVID-19 risk perception and to explore the influencing factors.

Methods

A total of 400 participants (200 who had experienced CMI and 200 who had not experienced) completed a questionnaire related to COVID-19 risk perceptions. The questionnaire was designed with the Cognitive-Experiential Self-Theory (CEST) and the Common Sense Model of Risk Perception (CSM). It adopted nine questions to measure risk perception in terms of Emotional feelings, Cognitive judgment, and Mental representation of unusual severity. Descriptive statistical analysis, correlation analysis, and multiple linear regression analysis were conducted with SPSS 26.0 software.

Results

The mean risk perception score for the CMI group was 30.75, with a standard deviation of 7.503, which was significantly higher than that in the non-centralized medical isolation (NCMI) group (risk perception score was 28.2, and the standard deviation was 7.129). The results show that risk perceptions were higher for older age, risk perceptions were higher for higher education, risk perceptions were higher for those who had received the COVID-19 vaccination, and risk perceptions were higher for those who lived in a family with children.

Conclusion

Risk perception is significantly higher in CMI groups than in NCMI groups. The government should draw more care to the risk perception and psychological wellbeing of the CMI group and provide extra support and assistance to the elderly and those raising younger children. In dealing with future pandemics like the COVID-19 outbreak, the government should actively guide the public to properly isolate at home and cautiously implement a CMI policy.
Combatting intergenerational effects of psychotrauma with multifamily therapy
Authors: Mooren Trudy , van Ee Elisa , Hein Irma , Bala Julia
Published: February 2023

There is growing evidence that parental trauma is associated with psychosocial disorders, externalizing and internalizing problems, and higher sensitivity to posttraumatic stress disorder (PTSD) in children. Recent research findings suggest multidimensional relational, psychological, and neurobiological interrelated pathways of intergenerational influence. Moreover, the intergenerational effects of parental trauma need to be understood within a broader systemic context, as a part of family adaptation. This article explores research findings and clinical practice to enhance our understanding of intergenerational processes and presents directions for therapeutic interventions. A trauma-focused multi-family therapy, aiming to restrict the relational consequences of parental trauma and strengthen family resilience, is described. The proposition is that to facilitate and improve the quality of parent–child interaction in response to psychotrauma, fostering emotion regulation capacities and mentalization is crucial. These efforts offered through family group interventions may benefit various families coping with adversity in culturally diverse societies.
The influence of price value on purchase intention among patients with chronic diseases in medical e-commerce during the COVID-19 pandemic in China
Authors: Han Linlin , Han Xu
Published: February 2023

Background

During the COVID-19 pandemic, medical e-commerce (MEC) has provided a way for patients with chronic diseases to purchase drugs online to maintain social distancing, decrease the risk of infection and community transmission, and relieve the burden on medical resources. Therefore, research which seeks to elucidate the drivers of purchase intention (PI) among patients with chronic diseases in MEC is vital. This study extended the theory of planned behavior (TPB) by integrating the price value (PV) variable into the original TPB framework and explored the effect of PV on patients' PI in MEC during the coronavirus pandemic.

Methods

Empirical data was gathered from 414 Chinese participants. Structural equation modeling was applied to explore the mechanism of chronic patients' PI in MEC. In addition, this study also estimated the moderating effect of gender, income, and region and the mediating role of attitude (ATT), subjective norm (SN), and perceived behavioral control (PBC) between PV and PI.

Results

Patients' PI in MEC is significantly affected by ATT, SN, and PBC. PV delivers significant influence on ATT, SN, PBC, and PI, with PV having the strongest effect on ATT. Gender, income, and region can significantly moderate the relationship between PV and ATT.

Conclusion

These findings can contribute to design targeted interventions to increase the adoption of MEC for patients with chronic diseases, decrease infection rates, and alleviate the strain on medical resources in the COVID-19 era.
Raising effective awareness for circular economy and sustainability concepts through students' involvement in a virtual enterprise
Authors: Keramitsoglou Kiriaki , Litseselidis Theodoros , Kardimaki Anna
Published: February 2023

Introduction

Despite increasing research on the transition of a well-established linear-oriented economic system toward the circular economy (CE) model, existing literature on the adoption and implementation of educational approaches that reinforce CE concept in secondary education seems to be limited. In light of the current challenges and the critical role of education in empowering students to explore new paths of sustainable development and grow into active citizens, conscious producers, and consumers, this study focuses on experiential learning as an effective tool for teaching CE and sustainability concepts. The literature review has revealed a research gap as regards the formulation of educational approaches to support CE concepts for secondary education students effectively, particularly in Greek vocational education. The present study describes and critically discusses how a virtual enterprise could introduce secondary-level students to the circularity and sustainability perspective, prepare them to build prosperity, and act circularly in the future.

Methods

Drawing on the activities of 32 students coming from different disciplines and participants in a virtual agri-business, we recommend practical educational strategies expecting (i) to encourage teachers to adopt innovative teaching methods and share good practices of CE teaching and (ii) to urge education policymakers to integrate the CE vision into school curricula.

Results and discussion

Furthermore, the students' responses to a structured questionnaire before and after the programme implementation indicate that experiential learning should be supported by the teaching of theoretical aspects for a better consolidation of abstract concepts such as CE and sustainability. The implementation of a circular enterprise by a student team consists of a positive prospect for the community and economy.
Human rights, COVID-19, and barriers to safe water and sanitation among people experiencing homelessness in Mexico City
Authors: Liera Carla , Dickin Sarah , Rishworth Andrea , Bisung Elijah , Moreno Alexia , Elliott Susan J.
Published: February 2023

Access to safe water, sanitation, and hygiene (WASH) are human rights and play a fundamental role in protecting health, which has been particularly evident during the SARS-CoV2 (COVID-19) pandemic. People experiencing homelessness face frequent violations of their human rights to water and sanitation, negatively affecting their health and dignity and ability to protect themselves from COVID-19. This research aimed to identify barriers to safe water, sanitation and hygiene access for people experiencing homelessness in Mexico City during the COVID-19 pandemic. A survey of 101 respondents experiencing homelessness was conducted using mobile data collection tools in collaboration with El Caracol A.C., an NGO that contributes to the visibility and social inclusion of homeless people in Mexico. We report findings according to the following themes: general economic impacts of COVID-19; experiences with reduced access to WASH services due to COVID-19, challenges in accessing hand washing to follow COVID-19 public health advice; and coping mechanisms used to deal with reductions in access to WASH. We discuss the broader implications of the findings in terms of realization of the human rights to water and sanitation (HRtWS), and how people experiencing homelessness are left behind by the existing approaches to ensure universal access to water and sanitation under SDG 6.
Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody
Authors: Zhu Wenjun , Pickering Bradley , Smith Greg , Pinette Mathieu , Truong Thang , Babiuk Shawn , Kobasa Darwyn , Banadyga Logan , Yang Ming
Published: February 2023

Introduction

Nipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality.

Methods

A competitive enzyme–linked immunosorbent assay (cELISA) using a monoclonal antibody (mAb) and recombinant NiV glycoprotein (G) was developed and laboratory evaluated using sera from experimental pigs, mini pigs and nonhuman primates. The test depends on competition between specific antibodies in positive sera and a virus–specific mAb for binding to NiV–G.

Results

Based on 1,199 negative and 71 NiV positive serum test results, the cutoff value was determined as 35% inhibition. The diagnostic sensitivity and specificity of the NiV cELISA was 98.58 and 99.92%, respectively. When testing sera from animals experimentally infected with NiV Malaysia, the cELISA detected antibodies from 14 days post–infection (dpi) and remained positive until the end of the experiment (28 dpi). Comparisons using the Kappa coefficient showed strong agreement (100%) between the cELISA and a plaque reduction neutralization test (PRNT).

Discussion

Because our cELISA is simpler, faster, and gives comparable or better results than PRNT, it would be an adequate screening test for suspect NiV and HeV cases, and it would also be useful for epidemiological surveillance of Henipavirus infections in different animal species without changing reagents.
Low-grade oncocytic tumour (LOT) of the kidney is characterised by GATA3 positivity, FOXI1 negativity and mTOR pathway mutations
Authors: Chen Tongbing , Peng Yan , Lei Ting , Wu Chao , Wang Hui , Shi Yongqiang
Published: February 2023

Aims: We present a 5-case series of low-grade oncocytic tumour of the kidney to further discuss their clinicopathological characteristics.

Methods and results: Five patients were included in this study. There were three females and two males aged 45–66 years, with a median age of 65 years. Four tumours were located in the right kidney, and one was located in the left kidney. Most of the tumour sections were yellow-brown in colour. Tumour sizes ranged from 2.5 to 4.5 cm, with a median size of 3 cm. Microscopically, the tumours were well-circumscribed but lacked a fibrous capsule; the tumours consisted of monomorphous oncocytic cells arranged mainly in solid and nested architectural patterns. The tumour cells had uniformly round to oval nuclei and often had perinuclear halos but lacked significant irregularities. Immunohistochemically, the tumour cells showed a diffuse and strong positivity for CK7 and were negative for CD117. The tumour cells were also positive for GATA3, E-cadherin, Pax-8, Succinate dehydrogenase B (SDHB) and Fumarate hydratase (FH), and negative for vimentin, Carbonic anhydrase 9 (CA9), CD10, P504s, CK20, TFE3, TFEB, HMB45, ALK and Forkhead box protein I1 (FOXI1). Next-generation sequencing identified genetic variations in these tumours, including MTOR gene mutations (4/5) and PIK3CA gene mutation (1/5). All patients were alive without disease progression at a median follow-up of 32 months (range 10–57 months).

Conclusion: LOT is an emerging renal entity of indolent behaviour that has morphologic overlap with some renal tumours with eosinophilic cytoplasm, primarily with oncocytoma and eosinophilic variant of chromophobe renal cell carcinoma. Familiarity with the distinctive morphological features, immunophenotype and molecular genetics of LOT helps avoid misdiagnosis.
The Guided Ultrasonic Wave Oscillation Phase Relation between the Surfaces of Plate-like Structures of Different Material Settings
Authors: Rittmeier Liv , Rauter Natalie , Mikhaylenko Andrey , Lammering Rolf , Sinapius Michael
Published: February 2023

Lamb waves occur in thin-walled structures in two wave modes—the symmetric and the antisymmetric mode. Their oscillation on the structures‘ surfaces is either in phase (symmetric) or shifted by a phase angle of π (antisymmetric). In this work, a method is developed by which to compare the surfaces’ oscillation phase relation to answer the question of whether fiber metal laminates show the same surface oscillation behavior as described for metals. The evaluation of time signals regarding the instantaneous phase angle is performed by using the continuous wavelet transformation and the short-time Fourier transformation. Numerical simulations utilizing the finite element method provide time signals from the top and bottom surface of different thin-walled structures of different material settings and configurations. The numerically obtained time signals are evaluated by the developed methods with respect to the oscillation phase. Subsequently, the oscillation phase is evaluated experimentally for the wave propagation in a fiber metal laminate. It is shown that the method based on the continuous wavelet transformation is suitable for the evaluation of oscillation phase relations in time signals. Additionally, it is proven that fiber metal laminates show only two phase relations, which indicates the occurrence of Lamb waves.
A Literature Review on Crack Arrest Features for Composite Materials and Composite Joints with a Focus on Aerospace Applications
Authors: Sioutis Ioannis , Tserpes Konstantinos
Published: February 2023

Crack propagation within composite materials or along the interface of composite joints is a phenomenon that might result in catastrophic failure of a structure. When the factor of safety is involved in the integrity of a structure, fail-safe design becomes crucial by embedding failure-confining features. This article reviews the research work that has been carried out on such crack-arresting features (CAFs) for composite laminates, composite-to-composite joints and composite-to-metal joints. The methodology of descriptive–narrative systematic literature review was employed in order to present the state of the research in the field. Crack stopping along adhesively joined interfaces was the most common subject encountered in the literature, while other types of secondary bonding such as thermoplastic welding were quite limited. The types of the CAFs were mainly categorized by means of their integration into the structure, namely “production” and “post-production”. For each method reviewed, the common aspects of the CAFs in question are discussed as well as the outcome of the work.
Antioxidant and Antibacterial Effect of Agaricus brasiliensis Extract on Raw and Cooked Pork Patties during Storage
Authors: Vargas-Sánchez Rey David , Torres-Martínez Brisa del Mar , Huerta-Leidenz Nelson , Ibarra-Arias Félix Joel , Fernández-López Juana , Torrescano-Urrutia Gastón Ramón , Perez-Alvarez José Ángel , Sánchez-Escalante Armida
Published: February 2023

Edible mushrooms have been proposed as a natural ingredient to prevent loss of quality in meat products. This study aimed to compare the antioxidant and antibacterial effects of Agaricus brasiliensis aqueous-ethanol extract (ABE, at 0, 0.5, and 1.0%) versus butylated hydroxytoluene (BHT, 0.02% on a fat basis) added to raw and cooked pork patties to prolong shelf-life under chilled storage. All samples were stored at 2 °C for 9 days and subjected to physicochemical (pH, water-holding capacity, and color), chemical (lipid oxidation and antioxidant status), and microbiological evaluation (mesophilic and psychrophilic). Phenolic compounds (TPC) in ABE exert a reducing power ability (Fe3+ reduction), free-radical (DPPH), and radical-cation scavenging activity (ABTS), as well as antibacterial activity against Gram-positive bacteria than Gram-negative. Furthermore, incorporating ABE in raw and cooked pork patties reduced (p < 0.05) pH and color changes, lipid oxidation, and microbial growth during storage in concentration dependence. No differences (p > 0.05) were observed in the WHC and b* values. In addition, the presence of TPC and the antioxidant status (Fe3+ reduction, DPPH, and ABTS activity) of pork patties increased (p < 0.05) by the ABE incorporation. ABE can be a natural additive to improve the storage stability of pork patties.
Analysis of Cyperus esculentus–Soil Dynamic Behavior during Rotary Tillage Based on Discrete Element Method
Authors: Zhao Zhuang , Wang Dongwei , Shang Shuqi , Hou Jialin , He Xiaoning , Gao Zenghui , Xu Nan , Chang Zengcun , Guo Peng , Zheng Xiaoshuai
Published: February 2023

Considering the problems of low soil fragmentation rates, high working resistance, and high energy consumption in the harvesting process of Cyperus esculentus in China, a method of Cyperus esculentus harvesting based on counter-rotation digging is proposed. The mechanism of interaction between the rotary tillage blade and Cyperus esculentus–soil is systematically investigated, and the vertical and horizontal disturbance performance of the positive and counter-rotating harvesting methods on soil and Cyperus esculentus is compared and analyzed. The results of the experiment showed that the intensity of soil and Cyperus esculentus disturbance by counter-rotation increased by 166.67% and 297.78%, respectively, and the effective disturbance time of soil and Cyperus esculentus increased by 133.33% compared to that of positive rotation. The working depth and rotation speed of the rotary tillage blade were the most significant for soil and Cyperus esculentus disturbance intensity. The working depth increased from 150 mm to 170 mm, and the soil disturbance intensity increased by 17.91% and 21.37% for positive and counter-rotating operation, respectively, and the rotation speed of the rotary tillage blade increased from 270 rpm to 330 rpm, and the soil disturbance intensity increased by 28.85% and 35.29%, respectively. Compared with the positive rotation operation, the Cyperus esculentus counter-rotation soil fragmentation rate increased by 4.09%, the Cyperus esculentus damage rate decreased by 10.69%, and the buried fruit rate decreased by 7.38%. This paper helps to understand the interaction mechanism between the rototiller and Cyperus esculentus–soil and lays a theoretical foundation for the subsequent design and optimization of the Cyperus esculentus digging device.
An In Situ Videotaping Approach for Parameterizing Subsoiling-Induced Soil Disturbance
Authors: Liang Lei , Sun Haotian , Ding Qishuo , He Ruiyin , Li Yinian , Xu Gaoming
Published: February 2023

Subsoiling (SS) is an important technology in conservation tillage, but soil-disturbance characteristics in the SS are rarely described. Research on soil-disturbance characteristics during SS is conducive to the design and optimization of subsoilers, which provides a basis for reducing draft force and energy consumption. This study conducted SS experiments at five different tillage depths in the field with a specific field in situ test-rig facility, and in situ videotaping was made from five positions during SS. The microrelief test, draft force test, disturbance cross-section test and disturbance process analysis were conducted after SS. The results showed that draft force increased with tillage depth as a quadratic function. Soil displacement parameters and soil crack parameters extracted from the video of SS were significantly correlated with tillage depth, which could be used for a quantitative description of the paddy soil-disturbance characteristics. Cross-sectional area showed a trend of “increasing then decreasing” with increasing tillage depth, reaching a maximum at a tillage depth of 20 cm. When the tillage depth was greater than 20 cm, the bottom of the disturbing boundary formed a “mole cavity”. Fallback rate was used to describe the change in disturbed height or width during and after SS, which exceeded 100% at maximum. The surface roughness of microrelief and the size of the average clods reached the maximum at tillage depth of 20 cm. Considering the shallow cultivation layer of paddy soil in rice–wheat rotation, the recommended tillage depth of 20 cm could achieve maximum soil disturbance and minimum energy consumed.
Short Communication: Spatial Dependence Analysis as a Tool to Detect the Hidden Heterogeneity in a Kenaf Field
Authors: Jang Gyujin , Kim Dong-Wook , Kim Hak-Jin , Chung Yong Suk
Published: February 2023

Ever since research attention was first paid to phenomics, it has mainly focused on the use of high throughput phenotyping for characterizing traits in an accurate and fast manner. It was recently realized that its use has huge potential in precision agriculture. However, the focus so far has mainly been on ”obtain large data set”, not on “how to analyze them”. Here, the expanded application of high throughput phenotyping combined with special dependence analysis is demonstrated to reveal the hidden field heterogeneity, using a kenaf field. Based on the method used in the study, the results showed that the growth of kenaf in the field was grouped into two, which led to a large variation of sources among replications. This method has potential to be applied to detect hidden heterogeneity, to be utilized and applied in plant breeding not only for better analysis, but also for better management of fields in precision agriculture.
Surgical and Bioengineering Integration in the Anatomy Course of Medicine and Surgery High Technology: Knowledge and Perception of Anatomy
Authors: Miglietta Selenia , Familiari Giuseppe , Relucenti Michela , Basili Stefania , Bini Fabiano , Bove Gabriele , Barbaranelli Claudio , Familiari Pietro
Published: February 2023

The Locomotor System Anatomy (LSA) course, placed in the first semester of the first year of the new Master’s degree in Medicine and Surgery High Technology (MSHT) at the Sapienza University of Rome, was integrated with surgical and bioengineering content. This study investigated the educational value and the students’ perceptions of the effectiveness of these two types of integration, comparing surgical integration (SI) with engineering integration (EI). Anatomy knowledge and students’ opinions attending the LSA course in MSHT degree (n = 30) were compared with those of students (n = 32) attending another medical and surgery course not comprising EI. Data show that students in the MSHT course like in-depth SI much more than in-depth EI. However, those who like in-depth SI also like in-depth EI. Significant differences were in anatomy knowledge between the two groups in the three sections of the test. There was no significant correlation between the three test scores and the levels of liking, while there was a significant correlation between students liking SI and those liking EI. A statistically significant correlation was also found in students who correctly responded to questions on the head and trunk, with students responding correctly to questions on the upper limbs. This study will be important in optimizing the deepening of SI and EI in the LSA course.
Characteristics of Physiological Parameters of Japanese Black Calves Relate to Carcass Weight
Authors: Arakawa Shotaro , Kim Minji , Aonuma Tatsuya , Takagi Michihiro , Watanabe Satoshi , Lee Huseong , Nishihara Koki , Haga Satoshi , Uemoto Yoshinobu , Roh Sanggun
Published: February 2023

This study aimed to identify the growth performance and blood factors associated with carcass weight in Japanese Black calves based on 675 performance tests and field carcass records. We measured the body weight, withers height, and chest girth at the start of fattening age (approximately 8–10 months) and analyzed eight blood factors, including vitamins and metabolites. Single- and two-trait animal models were used to estimate the heritability and genetic correlations. The heritability estimates for growth performance were moderate to high (ranging from 0.48 to 0.74), and those for blood metabolites were low to moderate (ranging from 0.19 to 0.51). Estimates for genetic correlations of carcass or body weight with body weight, withers height, and chest girth were high (ranging from 0.42 to 0.80). The body weight and withers height at 8 months of age are possibly closely related to the final carcass weight. The blood metabolites associated with body weight were vitamin E in steers (castrated males) and β-carotene in heifers. Our findings indicate that body measurements and blood metabolites measured during the growing period could be used to determine the nutritional and physiological status of cattle as well as predict carcass weight.
The Relationship between Knowing and Liking for 91 Urban Animal Species among Students
Authors: Sweet Fabio S. T. , Noack Peter , Hauck Thomas E. , Weisser Wolfgang W.
Published: February 2023

While there is growing consensus that nature should be promoted in cities, it is less clear what kind of nature this should be. One hypothesis is that humans show greater liking for those parts of nature that they know better. Using questionnaires, we studied the familiarity of 475 students with 91 urban animal species and the relationship between familiarity and attitudes towards the species. Students declared that they were familiar with most animals, but not all animals were equally liked. Better-known species were not generally the better-liked ones. The more familiar animal species were the more extreme attitudes became towards them, both positively and negatively. Our research shows that familiarity and attitude are not two sides of the same coin. It also emphasizes that there are parts of nature that are not liked by many humans and that this dislike is not necessarily correlated with insufficient knowledge. Detailed studies of what components of nature humans like and reasons underlying this are necessary to successfully increase nature in cities.
Soybean Replacement by Alternative Protein Sources in Pig Nutrition and Its Effect on Meat Quality
Authors: Parrini Silvia , Aquilani Chiara , Pugliese Carolina , Bozzi Riccardo , Sirtori Francesco
Published: February 2023

Soybean is one of the most expensive and limiting feed ingredients in diet formulations; however, in pig farming, it represents the main source of protein. The production and supply of soybean are critical steps due to their environmental impact and feed/food competition for land use. Therefore, research is focusing on finding alternatives to replace soybean partially or totally. However, alternative ingredients should ensure similar growth performance, carcass traits, and meat quality characteristics compared to conventional soybean-based diets. The objective of this review was to evaluate the impact of different alternative protein sources to soybean in pig nutrition and their effects on growth performance, carcass, and meat quality traits. The review process was performed on Scopus®, and it considered research findings published from 2012 to the present on the Sus scrofa species. Articles without a control group fed with soybean were discarded. The main alternative protein sources identified were other legumes and distillers’ dried grain with solubles (fish and animal proteins, oilseed by- and co-products). Interesting innovative protein sources included by-products from other industries (residues), microalgae and insects. Nevertheless, in dietary formulations, close attention must be paid to address the nutritional requirements, balance the supply of amino acids, avoid anti-nutritional or toxic compounds occasionally present in alternative protein sources, as well as determine the availability of protein feed in specific geographical areas.
To Cut the Mustard: Antimicrobial Activity of Selenocyanates on the Plate and in the Gas Phase
Authors: Sarfraz Muhammad , Nasim Muhammad Jawad , Gruhlke Martin C. H. , Handzlik Jadwiga , Jacob Claus
Published: February 2023

Organic selenocyanates (RSeCN) are among the most reactive and biologically active Se species, often exhibiting a pronounced cytotoxic activity against mammalian cells and microorganisms. Various aromatic selenocyanates have been synthesized and, similar to some of the most Reactive Sulfur Species (RSS), such as allicin, found to be active against a range of bacteria, including Escherichia coli, Pseudomonas syringae and Micrococcus luteus, and fungi, including Verticillium dahlia, Verticillium longisporum, Alternaria brassicicola, and Botrytis cinerea, even via the gas phase. The highest antimicrobial activity has been observed for benzyl selenocyanate, which inhibited the growth of all bacteria considerably, even at the lowest tested concentration of 50 µM. Notably, neither the analogues thiocyanate (BTC) nor isothiocyanate (BITC) show any of these activities, rendering this selenium motif rather special in activity and mode of action. Eventually, these findings advocate a range of potential applications of organic selenocyanates in medicine and agriculture.
A European International Multicentre Survey on the Current Practice of Perioperative Antibiotic Prophylaxis for Paediatric Liver Transplantations
Authors: Hauschild Juliane , Bruns Nora , Lainka Elke , Dohna-Schwake Christian
Published: February 2023

(1) Background: Postoperative infections are major contributors of morbidity and mortality after paediatric liver transplantation (pLTX). Evidence and recommendations regarding the most effective antimicrobial strategy are lacking. (2) Results: Of 39 pLTX centres, 20 responded. Aminopenicillins plus ß-lactamase inhibitors were used by six (30%) and third generation cephalosporins by three (15%), with the remaining centres reporting heterogenous regimens. Broad-spectrum regimens were the standard in 10 (50%) of centres and less frequent in the 16 (80%) centres with an infectious disease specialist. The duration ranged mainly between 24–48 h and 3–5 days in the absence and 3–5 days or 6–10 days in the presence of risk factors. Strategies regarding antifungal, antiviral, adjunctive antimicrobial, and surveillance strategies varied widely. (3) Methods: This international multicentre survey endorsed by the European Liver Transplant Registry queried all European pLTX centres from the registry on their current practice of perioperative antibiotic prophylaxis and antimicrobial strategies via an online questionnaire. (4) Conclusions: This survey found great heterogeneity regarding all aspects of postoperative antimicrobial treatment, surveillance, and prevention of infections in European pLTX centres. Evidence-based recommendations are urgently needed to optimise antimicrobial strategies and reduce the spectrum and duration of antimicrobial exposure.
Shedding Light on the Hidden Benefit of Porphyridium cruentum Culture
Authors: Liberti Davide , Imbimbo Paola , Giustino Enrica , D’Elia Luigi , Silva Mélanie , Barreira Luísa , Monti Daria Maria
Published: February 2023

Microalgae can represent a reliable source of natural compounds with different activities. Here, we evaluated the antioxidant and anti-inflammatory activity of sulfated exopolysaccharides (s-EPSs) and phycoerythrin (PE), two molecules naturally produced by the red marine microalga Porphyridium cruentum (CCALA415). In vitro and cell-based assays were performed to assess the biological activities of these compounds. The s-EPSs, owing to the presence of sulfate groups, showed biocompatibility on immortalized eukaryotic cell lines and a high antioxidant activity on cell-based systems. PE showed powerful antioxidant activity both in vitro and on cell-based systems, but purification is mandatory for its safe use. Finally, both molecules showed anti-inflammatory activity comparable to that of ibuprofen and helped tissue regeneration. Thus, the isolated molecules from microalgae represent an excellent source of antioxidants to be used in different fields.
An Original Asteraceae Based Infused Drink Prevents Metabolic Syndrome in Fructose-Rat Model
Authors: Idres Arezki Yanis , Tousch Didier , Dhuyque-Mayer Claudie , Hammad Imane , Lambert Karen , Cazals Guillaume , Portet Karine , Ferrare Karine , Bidel Luc. P. R. , Poucheret Patrick
Published: February 2023

Metabolic syndrome (METS) is a complex disorder that predisposes an affected person to an increased risk of diabetes and cardiovascular disease. Bitter Asteraceae plants contain several compounds active against METS that can be used as an alternative preventive therapy. Our previous work showed that a natural chicory extract (NCRAE) containing chicoric acid (CRA) and chlorogenic acid (CGA) in a molar ratio of 70/30 exhibited an antioxidant, insulin sensitization and anti-hyperglycemic effect. The present study was designed to evaluate the preventive effects of an NCRAE-like extract against METS in a complementary natural pharmacotherapeutic approach. An original Asteraceae infused drink containing the NCRAE CRA/CGA molecular ratio equivalent was prepared from dandelion (Taraxacum officinale L.) and burdock (Arctium lappa L.). The anti-METS effect of this drink was evaluated on the fructose-rat model for 8 weeks. Body weight, blood biochemistry, hepatic glucose-6-phosphatase, arterial blood pressure glucose and insulin tolerance were evaluated after 8 weeks. Our results show that daily oral intake of the Asteraceae infused drink led to a reduction of body weight gain, hyperglycemia, hypertriglyceridemia, insulin resistance and hypertension. Moreover, rat-by-rat analysis of the insulinemia measures revealed two types of responders. One sub-group of subjects demonstrated normal insulinemia and the other subgroup demonstrated hyperinsulinemia. This hyperinsulinemia, associated with the inhibition of the glucose-6-phosphatase activity in the liver tissue, may suggest an insulin release caused by CGA. The present study suggests that this original infusion of dandelion leaves and burdock roots may be used as an adjuvant therapy to prevent metabolic syndrome.
Effect of Resveratrol on Pregnancy, Prenatal Complications and Pregnancy-Associated Structure Alterations
Authors: Ramli Iman , Posadino Anna Maria , Giordo Roberta , Fenu Grazia , Fardoun Manal , Iratni Rabah , Eid Ali H. , Zayed Hatem , Pintus Gianfranco
Published: February 2023

Adverse pregnancy outcomes are considered significant health risks for pregnant women and their offspring during pregnancy and throughout their lifespan. These outcomes lead to a perturbated in-utero environment that impacts critical phases of the fetus’s life and correlates to an increased risk of chronic pathological conditions, such as diabetes, obesity, and cardiovascular diseases, in both the mother’s and adult offspring’s life. The dietary intake of naturally occurring antioxidants promotes health benefits and disease prevention. In this regard, maternal dietary intake of polyphenolic antioxidants is linked to a reduced risk of maternal obesity and cardio-metabolic disorders, positively affecting both the fetus and offspring. In this work, we will gather and critically appraise the current literature highlighting the effect/s of the naturally occurring polyphenol antioxidant resveratrol on oxidative stress, inflammation, and other molecular and physiological phenomena associated with pregnancy and pregnancy conditions, such as gestational diabetes, preeclampsia, and preterm labor. The resveratrol impact on prenatal complications and pregnancy-associated structures, such as the fetus and placenta, will also be discussed. Finally, we will draw conclusions from the current knowledge and provide future perspectives on potentially exploiting resveratrol as a therapeutic tool in pregnancy-associated conditions.