bluuebunny/crossref_metadata_2025 huggingface dataset:
Mesenchymal stem cells derived from adipose accelerate the progression of colon cancer by inducing a MT-CAFs phenotype via TRPC3/NF-KB axis
Authors: Xue Chunling , Gao Yang , Li Xuechun , Zhang Mingjia , Yang Ying , Han Qin , Sun Zhao , Bai Chunmei , Zhao Robert Chunhua
Published: July 2022

Background

There is increasing evidence that mesenchymal stem cells (MSCs) help shape the tumor microenvironment and promote tumor progression, and ion channels might play a critical role in this process. The objective of the present study was to explore the function and mechanism of MT-CAFs on progression of colon cancer.

Methods

Here, a gene chip was used for a general analysis of gene expression changes in MSC-transformed CAF cells (MT-CAFs). Bioinformatic tool and western blot screened out the ion channel protein TRPC3 with significantly increased expression, and identify the function through two-photon microscope. The progression of cancer was detected via MTS, transwell and Wound Healing. ELISA deected the secretion of inflammation factors. TRPC3/NF-KB axis was identified by western blot and immunofluorescence.

Results

TRPC3 can caused calcium influx, which further activated the NF-KB signaling pathway. Knockdown or inhibition of TRPC3 in MSCs significantly reduced the activation of NF-KB, and decreased the growth, migration, and invasion of MT-CAFs. After TRPC3 knockdown, the ability of MT- CAFs to promote tumor migration and invasion was impaired. Conversely, the upregulation of TRPC3 expression in MT-CAFs had the opposite effect. In vivo, TRPC3 expressed on MSCs also contributed to the tumorigenesis and progression of cancer cells. In addition, the Oncomine and GEPIA databases showed that TRPC3 expression is higher in colon cancer tissues compared with normal colon tissues, and was positively correlated with the expression of the CAF genes alpha-smooth muscle (α-SMA/ACTA2) and fibroblast activation protein Alpha. The disease-free survival of patients with positive TRPC3 expression in MSCs was significantly shorter than those with negative expression.

Conclusions

These results indicate that TRPC3 expressed on MT-CAFs plays a critical role in tumor progression via the NF-KB signaling pathway, and is correlated with poor prognosis in colon cancer patients. Therefore, TRPC3 may be a novel therapeutic target for the treatment of colon cancer.
Characterization of cardiac metabolism in iPSC-derived cardiomyocytes: lessons from maturation and disease modeling
Authors: Vučković Sofija , Dinani Rafeeh , Nollet Edgar E. , Kuster Diederik W. D. , Buikema Jan Willem , Houtkooper Riekelt H. , Nabben Miranda , van der Velden Jolanda , Goversen Birgit
Published: July 2022

Background

Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have emerged as a powerful tool for disease modeling, though their immature nature currently limits translation into clinical practice. Maturation strategies increasingly pay attention to cardiac metabolism because of its pivotal role in cardiomyocyte development and function. Moreover, aberrances in cardiac metabolism are central to the pathogenesis of cardiac disease. Thus, proper modeling of human cardiac disease warrants careful characterization of the metabolic properties of iPSC-CMs.

Methods

Here, we examined the effect of maturation protocols on healthy iPSC-CMs applied in 23 studies and compared fold changes in functional metabolic characteristics to assess the level of maturation. In addition, pathological metabolic remodeling was assessed in 13 iPSC-CM studies that focus on hypertrophic cardiomyopathy (HCM), which is characterized by abnormalities in metabolism.

Results

Matured iPSC-CMs were characterized by mitochondrial maturation, increased oxidative capacity and enhanced fatty acid use for energy production. HCM iPSC-CMs presented varying degrees of metabolic remodeling ranging from compensatory to energy depletion stages, likely due to the different types of mutations and clinical phenotypes modeled. HCM further displayed early onset hypertrophy, independent of the type of mutation or disease stage.

Conclusions

Maturation strategies improve the metabolic characteristics of iPSC-CMs, but not to the level of the adult heart. Therefore, a combination of maturation strategies might prove to be more effective. Due to early onset hypertrophy, HCM iPSC-CMs may be less suitable to detect early disease modifiers in HCM and might prove more useful to examine the effects of gene editing and new drugs in advanced disease stages. With this review, we provide an overview of the assays used for characterization of cardiac metabolism in iPSC-CMs and advise on which metabolic assays to include in future maturation and disease modeling studies.
Sex differences in contextual pattern separation, neurogenesis, and functional connectivity within the limbic system
Authors: Yagi Shunya , Lee Amanda , Truter Nadine , Galea Liisa A. M.
Published: July 2022

Background

Females are more likely to present with anxiety disorders such as post-traumatic stress disorder (PTSD) compared to males, which are associated with disrupted hippocampal integrity. Sex differences in the structure and function of hippocampus exist. Here, we examined sex differences in contextual pattern separation, functional connectivity, and activation of new neurons during fear memory.

Methods

Two-month-old male and female Sprague-Dawley rats were injected with the DNA synthesis markers, iododeoxyuridine (IdU) and chlorodeoxyuridine (CldU) 3 weeks and 4 weeks before perfusion, respectively. One week after CldU injection, the rats underwent a context discrimination task in which rats were placed in context A (shock) and context A’ (no shock) every day for 12 days. On the test day, rats were placed in the shock context (context A) to measure fear memory and expression of zif268, an immediate early gene across 16 different limbic and reward regions. Repeated-measures or factorial analysis of variance was conducted on our variables of interest. Pearson product-moment calculations and principal component analyses on zif268 expression across regions were also performed.

Results

We found that females, but not males, showed contextual discrimination during the last days of training. On the test day, both sexes displayed similar levels of freezing, indicating equivalent fear memory for context A. Despite similar fear memory, males showed more positive correlations of zif268 activation between the limbic regions and the striatum, whereas females showed more negative correlations among these regions. Females showed greater activation of the frontal cortex, dorsal CA1, and 3-week-old adult-born dentate granular cells compared to males.

Conclusions

These results highlight the importance of studying sex differences in fear memory and the contribution of adult neurogenesis to the neuronal network and may contribute to differences in susceptibility to fear-related disorders such as post-traumatic stress disorder.

Highlights

Female rats, but not male rats, show faster discrimination during a contextual pattern separation task.

Three-week-old adult-born neurons are more active in response to fear memory in females compared to males.

Females had greater neural activation compared to males in the frontal cortex and dorsal CA1 region of the hippocampus in response to fear memory.

Males and females show distinct patterns in functional connectivity for fear memory across limbic regions.

Males have many positive correlations between activated new neurons of different ages between the dorsal and ventral hippocampus, while females show more correlations between activated new neurons and other limbic regions.
Female mice exhibit less overall variance, with a higher proportion of structured variance, than males at multiple timescales of continuous body temperature and locomotive activity records
Authors: Smarr Benjamin , Kriegsfeld Lance J.
Published: July 2022

Despite recent work demonstrating that female rodents and humans do not show greater variance in behavior and physiology than males due to ovulatory cycles, many researchers still default to using males in their investigations. Although government funding agencies now require inclusion of female subjects where applicable, the erroneous belief that the study of males reduces overall data variance continues to result in male subject bias. Recently, we reported the first direct experimental refutation of this belief by examining continuous body temperature and locomotor activity in male and female mice. These findings revealed that males exceeded female variance within and across individuals over time, showing greater variance within a day than females do across an entire estrous cycle. However, the possibility remains that male variance within a day is impacted by ultradian rhythms, analogous to the influence of infradian estrous cycles on female variance, and both sexes show predictable, structured variance across the day. If structures underlying variance can be predicted, then the variance can be statistically accounted for, reducing experimental error and increasing precision of measurements. Here we assess these continuous body temperature and activity data for the contributions of structured and unstructured variance to overall variance within and across individuals at ultradian, circadian, and infradian timescales. In no instance do females exceed male variance, and in most instances male variance exceeds female variance. Additionally, more female variance is accounted for by temporal structure. In conclusion, even when estrous cycles are not controlled for, females show less variability than males, and this advantage can be further capitalized upon by inclusion of known temporal patterns to control for previously unknown but structured sources of variance.
Sex-biased and parental allele-specific gene regulation by KDM6A
Authors: Ma Wenxiu , Fang He , Pease Nicolas , Filippova Galina N. , Disteche Christine M. , Berletch Joel B.
Published: July 2022

Background

KDM6A is a demethylase encoded by a gene with female-biased expression due to escape from X inactivation. Its main role is to facilitate gene expression through removal of the repressive H3K27me3 mark, with evidence of some additional histone demethylase-independent functions. KDM6A mutations have been implicated in congenital disorders such as Kabuki Syndrome, as well as in sex differences in cancer.

Methods

Kdm6a was knocked out using CRISPR/Cas9 gene editing in F1 male and female mouse embryonic stem cells (ES) derived from reciprocal crosses between C57BL6 x Mus castaneus. Diploid and allelic RNA-seq analyses were done to compare gene expression between wild-type and Kdm6a knockout (KO) clones. The effects of Kdm6a KO on sex-biased gene expression were investigated by comparing gene expression between male and female ES cells. Changes in H3K27me3 enrichment and chromatin accessibility at promoter regions of genes with expression changes were characterized by ChIP-seq and ATAC-seq followed by diploid and allelic analyses.

Results

We report that Kdm6a KO in male and female embryonic stem (ES) cells derived from F1 hybrid mice cause extensive gene dysregulation, disruption of sex biases, and specific parental allele effects. Among the dysregulated genes are candidate genes that may explain abnormal developmental features of Kabuki syndrome caused by KDM6A mutations in human. Strikingly, Kdm6a knockouts result in a decrease in sex-biased expression and in preferential downregulation of the maternal alleles of a number of genes. Most promoters of dysregulated genes show concordant epigenetic changes including gain of H3K27me3 and loss of chromatin accessibility, but there was less concordance when considering allelic changes.

Conclusions

Our study reveals new sex-related roles of KDM6A in the regulation of developmental genes, the maintenance of sex-biased gene expression, and the differential expression of parental alleles.
Long-term outcomes in patients who received veno-venous extracorporeal membrane oxygenation and renal replacement therapy: a retrospective cohort study
Authors: Lumlertgul Nuttha , Wright Rebeka , Hutson Gareth , Milicevic Jovana Kusic , Vlachopanos Georgios , Lee Ken Cheah Hooi , Pirondini Leah , Gregson John , Sanderson Barnaby , Leach Richard , Camporota Luigi , Barrett Nicholas A. , Ostermann Marlies
Published: July 2022

Background

Acute kidney injury (AKI) is a frequent complication in patients with severe respiratory failure receiving extracorporeal membrane oxygenation (ECMO). However, little is known of long-term kidney function in ECMO survivors. We aimed to assess the long-term mortality and kidney outcomes in adult patients treated with veno-venous ECMO (VV-ECMO).

Methods

This was a single-centre retrospective study of adult patients (≥ 18 years old) who were treated with VV-ECMO at a commissioned ECMO centre in the UK between 1st September 2010, and 30th November 2016. AKI was defined and staged using the serum creatinine and urine output criteria of the Kidney Diseases: Improving Global Outcomes (KDIGO) classification. The primary outcome was 1-year mortality. Secondary outcomes were long-term mortality (up to March 2020), 1-year incidence of end-stage kidney disease (ESKD) or chronic kidney disease (CKD) among AKI patients who received renal replacement therapy (AKI-RRT), AKI patients who did not receive RRT (AKI-no RRT) and patients without AKI (non-AKI).

Results

A total of 300 patients [57% male; median age 44.5; interquartile range (IQR) 34–54] were included in the final analysis. Past medical histories included diabetes (12%), hypertension (17%), and CKD (2.3%). The main cause of severe respiratory failure was pulmonary infection (72%). AKI occurred in 230 patients (76.7%) and 59.3% received renal replacement therapy (RRT). One-year mortality was 32% in AKI-RRT patients vs. 21.4% in non-AKI patients (p = 0.014). The median follow-up time was 4.35 years. Patients who received RRT had a higher risk of 1-year mortality than those who did not receive RRT (adjusted HR 1.80, 95% CI 1.06, 3.06; p = 0.029). ESKD occurred in 3 patients, all of whom were in the AKI-RRT group. At 1-year, 41.2% of survivors had serum creatinine results available. Among these, CKD was prevalent in 33.3% of AKI-RRT patients vs. 4.3% in non-AKI patients (p = 0.004).

Conclusions

VV-EMCO patients with AKI-RRT had high long-term mortality. Monitoring of kidney function after hospital discharge was poor. In patients with follow-up creatinine results available, the CKD prevalence was high at 1 year, especially in AKI-RRT patients. More awareness about this serious long-term complication and appropriate follow-up interventions are required.
Three-dimensional SAR imaging with sparse linear array using tensor completion in embedded space
Authors: Zhang Siqian , Ding Ding , Zhao Chenxi , Zhao Lingjun
Published: July 2022

Due to the huge data storage and transmission pressure, sparse data collection strategy has provided opportunities and challenges for 3D SAR imaging. However, sparse data brought by the sparse linear array will produce high-level side-lobes, as well as the aliasing and the false-alarm targets. Simultaneously, the vectorizing or matrixing of 3D data makes high computational complexity and huge memory usage, which is not practicable in real applications. To deal with these problems, tensor completion (TC), as a convex optimization problem, is used to solve the 3D sparse imaging problem efficiently. Unfortunately, the traditional TC methods are invalid to the incomplete tensor data with missing slices brought by sparse linear arrays. In this paper, a novel 3D imaging algorithm using TC in embedded space is proposed to produce 3D images with efficient side-lobes suppression. With the help of sparsity and low-rank property hidden in the 3D radar signal, the incomplete tensor data is taken as the input and converted into a higher order incomplete Hankel tensor by multiway delay embedding transform (MDT). Then, the tucker decomposition with incremental rank has been applied for completion. Subsequently, any traditional 3D imaging methods can be employed to obtain excellent imaging performance for the completed tensor. The proposed method achieves high resolution and low-level side-lobes compared with the traditional TC-based methods. It is verified by several numerical simulations and multiple comparative studies on real data. Results clearly demonstrate that the proposed method can generate 3D images with small reconstruction error even when the sparse sampling rate or signal to noise ratio is low, which confirms the validity and advantage of the proposed method.
Generalized blending type Bernstein operators based on the shape parameter λ
Authors: Gezer Halil , Aktuğlu Hüseyin , Baytunç Erdem , Atamert Mehmet Salih
Published: July 2022

In the present paper, we construct a new class of operators based on new type Bézier bases with a shape parameter λ and positive parameter s. Our operators include some well-known operators, such as classical Bernstein, α-Bernstein, generalized blending type α-Bernstein and λ-Bernstein operators as special case. In this paper, we prove some approximation theorems for these operators. Approximation properties of our operators are illustrated on graphs for variables s, α, λ, and n. It should be mentioned that our operators for $\lambda =1$
λ
=
1
have better approximation than Bernstein and α-Bernstein operators.
Effectiveness of indoor residual spraying on malaria control: a systematic review and meta-analysis
Authors: Zhou Yiguo , Zhang Wan-Xue , Tembo Elijah , Xie Ming-Zhu , Zhang Shan-Shan , Wang Xin-Rui , Wei Ting-Ting , Feng Xin , Zhang Yi-Lin , Du Juan , Liu Ya-Qiong , Zhang Xuan , Cui Fuqiang , Lu Qing-Bin
Published: July 2022

Background

Indoor residual spraying (IRS) is one of the key interventions recommended by World Health Organization in preventing malaria infection. We aimed to conduct a systematic review and meta-analysis of global studies about the impact of IRS on malaria control.

Method

We searched PubMed, Web of Science, Embase, and Scopus for relevant studies published from database establishment to 31 December 2021. Random-effects models were used to perform meta-analysis and subgroup analysis to pool the odds ratio (OR) and 95% confidence interval (CI). Meta-regression was used to investigate potential factors of heterogeneity across studies.

Results

Thirty-eight articles including 81 reports and 1,174,970 individuals were included in the meta-analysis. IRS was associated with lower rates of malaria infection (OR = 0.35, 95% CI: 0.27–0.44). The significantly higher effectiveness was observed in IRS coverage ≥ 80% than in IRS coverage < 80%. Pyrethroids was identified to show the greatest performance in malaria control. In addition, higher effectiveness was associated with a lower gross domestic product
as well as a higher coverage of IRS and bed net utilization.

Conclusions

IRS could induce a positive effect on malaria infection globally. The high IRS coverage and the use of pyrethroids are key measures to reduce malaria infection. More efforts should focus on increasing IRS coverage, developing more effective new insecticides against malaria, and using multiple interventions comprehensively to achieve malaria control goals.
Monkeypox claims new victims: the outbreak in men who have sex with men
Authors: Liu Xiaoning , Zhu Zheng , He Yun , Lim Jia Wen , Lane Bethany , Wang Hui , Peng Qiaoli , Sun Liqin , Lu Hongzhou
Published: July 2022

Monkeypox has a very prominent regional epidemic. It has been confined to Western and Central African countries. Sporadic cases found in countries outside Africa generally have a history of sojourn in endemic areas. However, the recent multinational outbreak of monkeypox cases in Europe in early May 2022 has revealed a changing epidemiological trend, those confirmed cases had no sojourn history in endemic areas and with a high proportion of cases involving men who have sex with men (MSM). Among the MSM cases, many of them presented atypical clinical manifestations of monkeypox and with other sexually transmitted diseases co-infection. Combined with the high social interactivity in this community, there is likely a higher risk of monkeypox transmission in this population. Establishing an infectious disease surveillance system, maintaining highly vigilant regarding the transmission of monkeypox in MSM, and responding promptly are necessary and effective measures to contain the outbreak.
An empirical evaluation of The Resilience Shield model
Authors: Notebaert Lies , Abdul Razak Hannah , Masschelein Stijn
Published: July 2022

Background

Resilience refers to the process through which individuals deal with the adversity they experience. Previous research has shown there are multiple factors that contribute to individuals’ resilience, leading to increasing interest in the development of multidimensional resilience models. Once such recently proposed model is The Resilience Shield, which clusters groups of protective factors into different shield layers. The stronger these layers, the better the protection against adversity (Pronk et al. in The Resilience Shield, Pan Macmillan Australia, 2021). While this model was based in part on existing literature, no empirical evaluation has occurred to date. The aim of this study was therefore to evaluate the model fit for each of the modifiable shield layers and the overall model, and to examine whether each of the constructs included contributes to observed resilience scores.

Methods

Participants completed a series of questionnaires via The Resilience Shield website assessing constructs relevant to each resilience shield layer. Data from 3337 participants was analysed using Structural Equation Modelling and regression analyses.

Results

The results showed acceptable fit of the measurement model for the Social, Mind, and Professional Layers, but poor fit for the Body Layer. There was also good fit for the overall model. In addition, all but one of the constructs included in The Resilience Shield survey explained independent variance in either dispositional resilience scores, or dispositional vulnerability scores.

Conclusion

These results broadly support the multidimensional structure proposed by The Resilience Shield model and suggest that (at least in the population in which it was tested) this may be an acceptable model to index individuals’ performance on a range of indicators that contribute to resilience.
Cytotoxic and apoptotic potential of gemini-chrysophanol nanoparticles against human colorectal cancer HCT-116 cell lines
Authors: Naqishbandi Alaadin M.
Published: July 2022

Background

Colorectal cancer is among the most common cancers and accounts for nearly 9% of all cancers in the world. Chrysophanol ﻿is a naturally occurring anthraquinone exerts a number of ﻿pharmacological activities ﻿such as anti-inflammation, anti-cancer, ﻿anti-bacterial, anti-viral, and anti-oxidant effects. ﻿This study aims to ﻿produce a novel gemini chrysophanol nanoparticles (Gemini-Chr NPs), and ﻿to evaluate its anti-cancer effect on the human colorectal cancer cell lines.

Methods

Gemini-Chr NPs were synthesized through nanoprecipitation method and characterized by dynamic light scattering and scanning electron microscopy, ﻿Anti-cancer activities were examined through MTT assay on HCT-116 cancer cells, apoptosis was investigated via Annexin V-FITC/PI dual stain assay. Furthermore, the expression of Bax, Bcl-2 and P53 genes were evaluated using real-time PCR and western blotting assay. ﻿

Results

The average particle diameter of the synthesized Gemini-Chr NPs and zeta potential were recorded as 120 nm and 14.4 mV, respectively. In comparison to the normal cells, the cytotoxicity assay confirmed that Gemini-Chr NPs preferentially killed colorectal cancer cells via induction of apoptosis. ﻿Moreover, Gemini-Chr NPs could upregulate the expression of Bax in both cancerous and normal cells (p ≤ 0.05) and decreasing the Bcl-2 expression in only tumor cells (p ≤ 0.01), while the expression of P53 is modulated in tumor cells (p ≤ 0.05).

Conclusions

Gemini surfactants could be considered for efficient delivery and improvement of anti-cancer effect of chrysophanol. Gemini-Chr NPs might have the potential for developing novel therapeutic agent against colorectal cancer.
Intelligent Physical Exercise Training (IPET) in the offshore wind industry: a feasibility study with an adjusted conceptual model
Authors: Oestergaard Anne Skov , Sandal Louise Fleng , Smidt Trine Fernando , Søgaard Karen
Published: July 2022

Background

Good physical health and capacity is a requirement for offshore wind service technicians (WTs) who have substantial physical work demands and are exposed to numerous health hazards. Workplace physical exercise has shown promise for improving physical health and work ability among various occupational groups. Therefore, we aimed to assess the feasibility and preliminary efficacy of Intelligent Physical Exercise Training (IPET) among WTs in the offshore wind industry.

Methods

A within-subject design was used to assess the feasibility and preliminary efficacy of IPET (one hour/week individualized exercise during working hours). The intervention period was 12 weeks, with the first eight weeks performed on site as supervised or partly supervised exercise during work hours and the last four weeks planned as home-administered exercise after the seasonal offshore service period. Three assessments, T1 (six months prior to intervention start), T2 (start of intervention) and T3 (end of intervention), of physical health and capacity (self-reported and objective measurements) were conducted and the period between T1 and T2 served as a within-subject control period. Primary outcome was feasibility measured as compliance, adherence, adverse events, and participant acceptability. Descriptive statistics were used to present feasibility outcomes. Preliminary efficacy was reported as mean differences with 95% confidence intervals for health and physical capacity outcomes between T1 and T2, between T2 and T3 and between T1 and T3.

Results

All WTs at the included wind farm (n=24, age: 40 years (SD±8)) participated in the study. No serious adverse events were reported. Compliance and adherence of 95 and 80% respectively, were reached in the eight-week supervised part, but were lower when exercise was home-administered (<20%). Acceptability was high for the supervised part, with 83% indicating that the exercise program worked well and 100% that exercise should be implemented as an integrated part of the working structure. Changes in physical capacity and health indicators, such as VO2max (ml O2/kg/min) at T1 (38.6 (SD±7.2)), T2 (44.1 (SD±9)) and T3 (45.8 (SD±6.5)), may indicate seasonal fluctuations as well as improvements from the intervention.

Conclusion

On-site Intelligent Physical Exercise Training during working hours was feasible and well received among WTs in the offshore wind industry. The proceeding of larger-scale evaluation and implementation is therefore recommended.

Trial registration

ClinicalTrials.gov
(Identifier: NCT04995718). Retrospectively registered on August 6, 2021,
Bringing the patient voice into the operating room: engaging patients in surgical safety research with the Operating Room Black Box®
Authors: Etherington Cole , Lê Maxime , Proulx Laurie , Boet Sylvain
Published: July 2022

Background

Surgery is one of the most common patient experiences in the health care system. Yet, efforts to engage patients in surgical safety research have not matched those of other health care fields. This is a critical issue given the nature of surgery inhibits patients’ abilities to advocate for themselves as they are typically under anesthetic when the procedure is performed. We partnered with patients throughout our research program, which uses the Operating Room Black Box® to enhance surgical patient safety through transparent and proactive analysis of human factors to detect and prevent avoidable errors.

Main body

In this article, we outline the need for, and our approach to, patient engagement in surgical safety research. Our approach included a series of planned activities and skill development opportunities designed to build capacity and bring together patients, clinicians, and researchers to inform research and practice. We also conducted evaluation surveys during the first year of our program, which have indicated a positive experience by both patient partners and the research team.

Conclusion

We believe our approach can serve as an important first step toward building a model for patient engagement in the surgical safety field and could significantly contribute to improved quality of care and outcomes for surgical patients.
Development of new copper-64 labeled rhodamine: a potential PET myocardial perfusion imaging agent
Authors: AlHokbany Norah , AlJammaz Ibrahim , AlOtaibi Basem , AlMalki Yousif , AlJammaz Bander , Okarvi Subhani M.
Published: July 2022

Background

Myocardial perfusion imaging (MPI) is one of the most commonly performed investigations in nuclear medicine procedures. Due to the longer half-life of the emerging positron emitter copper-64 and its availability from low energy cyclotron, together with its well-known coordination chemistry, we have synthesized 64Cu-labeled NOTA- and 64Cu-NOTAM-rhodamine conjugates as potential cardiac imaging agents using PET.

Results

64Cu-NOTA- and 64Cu-NOTAM-rhodamine conjugates were synthesized using a traightforward and one-step simple reaction. Radiochemical yields were greater than 97% (decay corrected), with a total synthesis time of less than 25 min. Radiochemical purities were always greater than 98% as assessed by TLC and HPLC. These synthetic approaches hold considerable promise as a simple method for 64Cu-rhodamine conjugates synthesis, with high radiochemical yield and purity. Biodistribution studies in normal Fischer rats at 60 min post-injection, demonstrated significant heart uptake and a good biodistribution profile for both the radioconjugates. However, the 64Cu-NOTAM-rhodamine conjugate has shown more heart uptake (~ 10% ID/g) over the 64Cu-NOTA-rhodamine conjugate (5.6% ID/g).

Conclusions

These results demonstrate that these radioconjugates may be useful probes for the PET evaluation of MPI.
Comparative responsiveness and minimally important difference of Fatigue Symptom Inventory (FSI) scales and the FSI-3 in trials with cancer survivors
Authors: Mosher Catherine E. , Secinti Ekin , Johns Shelley A. , Kroenke Kurt , Rogers Laura Q.
Published: July 2022

Background

Fatigue is a highly prevalent and disabling symptom in cancer survivors. Although many measures have been developed to assess survivors’ fatigue, their ability to accurately capture change following intervention has rarely been assessed in post-treatment survivors. Ultra-brief fatigue measures are preferable in clinical practice but have limited evidence supporting their use with cancer survivors. We examined the psychometric properties of four Fatigue Symptom Inventory (FSI) measures, including the new FSI-3, in cancer survivors. Examined properties included responsiveness to change and minimally important differences (MIDs).

Methods

We analyzed data from three randomized controlled trials with post-treatment cancer survivors (N = 328). Responsiveness to change was evaluated by comparing standardized response means for survivors who reported their fatigue as being better, the same, or worse at 2–3 months. Responsiveness to intervention was assessed via effect sizes, and MIDs were estimated by using several methods. We also computed area under the curve (AUC) values to assess FSI measures’ discriminative accuracy compared to an established cut-point.

Results

All FSI measures differentiated survivors who reported improvement at 2–3 months from those with stable fatigue, but did not uniformly differentiate worsening fatigue from stable fatigue. Measures showed similar levels of responsiveness to intervention, and MIDs ranged from 0.29 to 2.20 across FSI measures. AUC analyses supported the measures’ ability to detect significant fatigue.

Conclusions

Four FSI scales show similar responsiveness to change, and estimated MIDs can inform assessment of meaningful change in fatigue. The FSI-3 shows promise as an ultra-brief fatigue measure for survivors.
Can patient-reported outcome measures be used to predict consultation needs in patients with psoriasis?: A survey study
Authors: Krontoft Anna Sophie Belling , Jensen Johanna Walsøe , Pedersen Mette Charlotte , Pors Maria , Kaur-Knudsen Diljit , Zachariae Claus , Skov Lone
Published: July 2022

Background

Patient-reported outcome measures (PROMs) are emerging tools used to capture a patient’s daily health status and enhance communication between patients and healthcare professionals. This study examined whether PROMs can be used to predict consultation needs in an outpatient clinic setting including patients diagnosed with psoriasis.

Method

We evaluated a nationally developed set of PROMs for psoriasis patients, which included a standard set of questionnaires that capture patients’ perceptions of their experience and quality of life. Patients (n = 187) answered the psoriasis PROMs prior to an in-person consultation. Their responses were evaluated alongside patient, doctor, and nurse opinions on whether the subsequent consultation was necessary. Additionally, comments about the consultations from the patient, doctor, and nurse were collected and provided insights as to why certain consultations were deemed necessary.

Results

Comparing the patient, doctor, and nurse responses addressing a need for consultation compared to the coded psoriasis PROMs results (red or green/yellow outcome), 23% of the patients with a green/yellow outcome were in need of a doctor’s consultation. Upon considering a subset of psoriasis PROMs questionnaires that reflect subjective responses (e.g., DLQI, PEST, MDI-2, and side effects), the proportion of patients that yielded a green/yellow outcome and were identified to require a doctor consultation increased to approximately 45%.

Conclusions

The preliminary results show that the psoriasis PROMs were supportive in the consultation but alone cannot sufficiently guide healthcare professionals to determine whether in-person consultations are required.
Portal vein embolization with absolute ethanol to induce hypertrophy of the future liver remnant
Authors: Santhakumar Cositha , Ormiston William , McCall John L , Bartlett Adam , Duncan David , Holden Andrew
Published: July 2022

Background

Preoperative portal vein embolization (PVE) is widely used prior to major liver resection to reduce the risk of post-hepatectomy liver failure (PHLF). We evaluated the efficacy and safety of PVE using absolute ethanol.

Methods

Consecutive patients undergoing preoperative PVE between February 2003 and February 2020 at a high-volume tertiary institution were retrospectively reviewed. Hypertrophy of the future liver remnant (FLR) was determined by comparing volumetric data using semi-automated software on computed tomography or magnetic resonance imaging before and after PVE. Efficacy of absolute ethanol was evaluated by the percentage increase in the FLR volume and the ratio of the FLR to the total liver volume (TLV). Technical success and complications following PVE were evaluated. Feasibility of hepatectomy following PVE and the incidence of PHLF were determined.

Results

Sixty-two patients underwent preoperative PVE using absolute ethanol. The technical success rate was 95.2%. Median time interval between PVE and follow-up imaging was 34 days (range 6–144 days). The mean increase in FLR volume and ratio of the FLR to TLV were 43.6 ± 34.4% and 12.3 ± 7.7% respectively. Major adverse events occurred in 3 cases (4.8%) and did not preclude consideration of surgery. Forty-two patients (67.8%) proceeded to surgery for intended hepatectomy of which 36 patients (58.1%) underwent liver resection. Major post-operative complications occurred in 4 patients (11.1%) and there were no cases of PHLF.

Conclusion

Preoperative PVE with absolute ethanol is effective and safe in inducing hypertrophy of the FLR before partial hepatectomy to prevent PHLF.
Profiling of insulin and resveratrol interaction using multi-spectroscopy and molecular docking study
Authors: Yanti Sahri , Chien Wei-Jyun , Agrawal Dinesh Chandra
Published: July 2022

Background

Resveratrol, a phenolic compound, has various medicinal properties, including anticancer, anti-diabetic, antioxidant, anti-inflammatory, etc. Diabetes is a killing disease, especially for people who cannot maintain a healthy lifestyle. People with diabetes need additional information about a supplement that can prevent and treat diabetes. The present study aims to investigate the interaction of insulin with resveratrol using fluorescence spectroscopy, UV–Vis spectroscopy, CD spectropolarimeter, and molecular docking methods. As an outcome of this study, we expect to understand the contribution of resveratrol in insulin resistance.

Result

The fluorescence spectroscopy results showed that the peak intensity of insulin emission decreased with resveratrol. The interaction of insulin with resveratrol involved a combination of static and dynamic quenching effects. Temperature changes caused the binding constant (K) and the binding site (n) unstable. The interaction occurred through hydrogen bonding, van der Waal, and was hydrophobic. The results of UV–Vis spectroscopy showed that the addition of resveratrol caused a peak in a blueshift, and the absorbance was hyperchromic. Also, there was a reduction in electron transition and the extinction coefficient. The CD spectropolarimeter results showed that the addition of resveratrol affected the secondary structure of insulin. The amount of α-helix and β-sheet slightly change and increase in the secondary structure’s length. The molecular docking study showed that resveratrol interacts via hydrogen bonding with glycine and asparagine. van der Waal interactions occurred in asparagine, phenylalanine, and cysteine. The interaction of electrons occurred through the π orbitals of resveratrol with tyrosine A and B. The binding energy of molecules interaction happened spontaneously on a hydrophobic surface.

Conclusion

Profiling the interaction of insulin and resveratrol shows that resveratrol can stabilize insulin structure and prevent insulin resistance in diabetes.
Thyrotoxicosis in Africa: a systematic review and meta-analysis of the clinical presentation
Authors: Azeez Taoreed Adegoke , Adetunji Tajudin Adesegun , Adio Mosunmoluwa
Published: July 2022

Background

Thyrotoxicosis is a common endocrine disorder. The clinical presentation is variable, and it is often misdiagnosed or diagnosed late in Africa. This study was aimed at collating and analyzing the clinical characteristics of the disease across the continent so as to enhance correct and timely diagnosis.

Methods

The study is a systematic review with a meta-analysis. Studies, done in Africa, which documented the clinical features of thyrotoxicosis were selected. African Journal Online (AJOL), PubMed, SCOPUS and Google Scholar, Research Square, SciELO, and medRxiv were systematically searched using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The study quality was assessed using the Newcastle-Ottawa scale. Heterogeneity was determined using I2 statistic and Cochran’s Q test. LFK index and the symmetry of the Doi plot were used to assess publication bias.

Results

The eligible studies were 59 and the total sample size was 9592. The most common symptoms of thyrotoxicosis on the continent included palpitations (69%), weight loss (65%), heat intolerance (64%), tiredness (49%), increased appetite (49%), hyperhidrosis (48%), and insomnia (47%). The most common signs were thyromegaly (88%), tachycardia (67%), sweaty palms (54%), hand tremor (49%), and exophthalmos (49%). Atrial fibrillation, heart failure, and thyrotoxic heart disease were found in 9, 12, and 22% respectively. Other findings were hypertension (25%) and diabetes (9%).

Conclusion

Clinical presentation of thyrotoxicosis varies, and understanding these peculiarities would mitigate misdiagnosis and delayed diagnosis in Africa.
Serum fibrinogen to albumin ratio in patients with ankylosing spondylitis: correlation with disease activity and severity
Authors: Ibrahim Noha Hosni , El Hanafy Mounir Serag-El Deen , El Brashy Abdel Wahab Shams-El Deen , Abd-Elmonem Enas Adel
Published: July 2022

Background

The fibrinogen to albumin ratio (FAR) has risen to prominence as a novel biomarker for various conditions, including systemic inflammation. Therefore, this study aims to assess the role of FAR in ankylosing spondylitis (AS) and its correlation with disease activity, severity, and functional status of AS patients. Thirty adult patients with AS were enrolled, along with 20 healthy age- and sex-matched subjects as controls. Bath Indices were used to determine the disease activity, severity, and functional status.

Results

The patients’ mean age was 35.3 years, including 22 males (73.3%) and eight females (26.7%). The median disease duration was 10 years, ranging from 1 to 28 years. AS cases showed higher FAR than the control group (P < 0.001). Active cases were associated with significantly higher FAR when compared to cases in remission (P < 0.001). FAR showed significant positive correlations with Bath Indices including BASDAI, BASFI, and BASMI scores (P = 0.002, 0.002, < 0.001, 0.019, 0.022).

Conclusion

FAR has been increased in AS, particularly in active cases. FAR has also been associated with the impaired functional status of AS patients. FAR can represent a novel inflammatory parameter for monitoring disease activity and severity in AS.
Performance evaluation of electric discharge machining of titanium alloy-a review
Authors: Rathod Rajusing , Kamble Dinesh , Ambhore Nitin
Published: July 2022

Titanium alloy has a high specific resistance, excellent machining performance is non-corrosive, and the capability to withstand greater temperatures while maintaining outstanding mechanical properties. This alloy is, therefore, the right choice for aerospace, maritime, biomedical, and industrial applications. But machinability of titanium alloy is challenging as a result of its poor thermal conductivity, highly chemically reactive, and low elastic modulus hence it is treated as a difficult-to-cut material. Fast tool wear is observed during the machining of titanium alloy in conventional machining methods. Therefore, unconventional processing methods are used for the treatment of titanium alloy. Electric discharge machining (EDM) is one of these unconventional machining processes which are used for cutting with high precision, having a high degree of machinability, and getting a better surface finish. It is considered the best choice for machining titanium alloy. In the EDM process, different techniques are used to understand the effects of process parameters such as polarity, peak current, electrode type, pulse on time, and gap voltage on material removal rate, tool wear rate, surface roughness, and wear ratio. This paper critically investigates different types of EDM processes, experimental setups used for machining of titanium alloy, the effect of different tool electrodes and dielectric media on machining parameters, machined surface characteristics, and metal removal rate and tool wear rate.
Bio-efficacy of Mangifera leaf extracts on mortality of Aedes aegypti and inhibition of egg hatching
Authors: Mahdi Nur , Ridha Muhammad Rasyid , Setiawan Deni , Praristiya Muhammad Riki Shindi , Rahayu Nita , Atmaja Bayu Purnama
Published: July 2022

Background and Aim: To develop an environmentally friendly alternative to mosquito larvicides for vegetables, leaf extracts of Mangifera laurina, Mangifera casturi, Mangifera indica, Mangifera odorata, Mangifera caesia, and Mangifera foetida were prepared. This study aimed to determine the biological efficacy of several Mangifera leaf extracts on the mortality of Aedes aegypti mosquito and the inhibition of egg hatching.

Materials and Methods: Extraction was performed in an organic solvent (methanol) using a Soxhlet extractor. The larvicidal potential of six leaves of Mangifera essential oil was evaluated against the third instar larvae of A. aegypti at concentrations of 1500, 2000, 3000, and 5000 ppm using the World Health Organization protocol. After Probit analysis, the 48 h LC50 and LC90 values of the essential oils were determined. The inhibitory effect on egg hatching was also tested at 160, 320, 480, and 640 ppm.

Results: The extraction of essential oils from several Mangifera species had excellent larvicidal activity and inhibitory activity against A. aegypti egg hatching. The LC50/LC90 values were: M. casturi, 241/1964 ppm; M. laurina, 2739/4035 ppm; and M. caesia, 1831/2618 ppm. The inhibitory effect on hatching was 78% for M. foetida, 70% for M. caesia, and 59% for M. casturi.

Conclusion: The test results indicate the potential of some Mangifera species for use as larvicides and inhibitors of egg hatching; thus, they have the potential to control A. aegypti in the early stages of development.
Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia
Authors: Fikri Faisal , Wardhana Dhandy Koesoemo , Purnomo Agus , Khairani Shafia , Chhetri Shekhar , Purnama Muhammad Thohawi Elziyad
Published: July 2022

Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the prevalence and the presence of the aerolysin gene and antimicrobial resistance profile of A. hydrophila isolated from milkfish in Gresik, Indonesia.

Materials and Methods: A total of 153 milkfish gill samples were collected from 16 locations in Gresik and then cultured and identified using biochemical tests. The aerolysin gene was investigated using a polymerase chain reaction, and antimicrobial resistance profiles of the recovered isolates were investigated.

Results: Of the 153 examined samples, 35 (22.9%) were confirmed positive for A. hydrophila and 22 (62.9%) presented the aerolysin gene. The recovered isolates were resistant to the following antibiotics: Amoxicillin (62.9%), tetracycline (60%), streptomycin (54.3%), cefotaxime (51.4%), gentamycin (31.4%), kanamycin (28.6%), erythromycin (25.7%), chloramphenicol (20%), and trimethoprim (14.3%). Meanwhile, only ciprofloxacin, nalidixic acid, and imipenem were indicated as susceptible.

Conclusion: The presence of the aerolysin gene is vital in determining the virulence of A. hydrophila. The study results indicated a high aerolysin gene prevalence. In addition, this study emphasized antibiotic use monitoring, food safety improvement, and negative impact reduction on human health and the environment.
ISLAM, PEREMPUAN, DAN WACANA MODERNITAS DALAM RATU YANG BERSUJUD KARYA MAHDAVI DAN SERIAL JILBAB TRAVELER KARYA ASMA NADIA
Authors: Susanto Dwi , Nugroho Miftah , Wati Rianna
Published: July 2022

The issue of Islam, women, and modernity discourses are common discussions in Indonesian literature. Literary works can be seen as part of an effort to welcome these ideas. This study discusses the literary works of Mahdavi and Asma Nadia in responding to issues of Islam, women, and modernity discourse. This study uses a postcolonial study point of view. The object of this research is Ratu Yang Bersujud (2013) by Mahdavi and Hijab Traveler (2012, 2014, and 2015) series from Asma Nadia. The formal object is the discourse of colonial modernity, Islam, and women. The data of this research are the contents of literary works, colonial discourse, Islam, women, and modernity. The data interpretation technique was carried out by following the binary opposition reading procedure in postcolonial studies. The response to the discourse on women, Islam, and modernity is done by negotiating and at the same time building women's constructions. However, women's voices become ambiguous when the subject is trapped in the discourse of modernity and capitalism through the issue of globalization. Women must continue to play their Islamic identity in entering the arena of modernity.