bluuebunny/crossref_metadata_2025 huggingface dataset:
Management Of Early Children's Learning With Steam Loading With Loose Parts at RA Ummatan Wahidah Curup
Authors: Rahayu Cucu , Warlizasusi Jumira , Fakhruddin Fakhruddin
Published: January 2023

Implementation of  Early Childhood Education faces many problems in the learning system. Just as the lack of creativity and progress of educators, as well as educational game devices that are less supportive, so the learning system does not encourage the ability of students to innovate and think higher. The research objectives are learning management, and implementation of STEAM content made from Loose Parts. The research used descriptive qualitative method. Research data sources include primary and secondary sources. The research instrument, data collection using observation, interview, and documentation techniques. Then the data is analyzed through the stages of data collection, data reduction, data display, and conclusions. The results of this study that the management of STEAM content learning with Loose Parts : planning the form of learning for RPPS, RPPM, RPPH, preparing Loose Parts . Organizing teaching tasks, teachers allocate learning materials. Implementation starts from the introduction, core activities, closing activities. Assessment in the form of performance, observation, conversation. Implementation of early childhood learning with STEAM content made from Loose Parts : applying all stages of play by paying attention to playing strategies, cleaning, storing things, strategies to develop early childhood creativity. The activity plan is adapted to the needs of the child and takes into account the principles of PAUD learning
The Place of Information and Communication Technology in the Effective Teaching and Learning of History in the Nigerian Educational Institutions In The 21st Century
Authors: Adesote Adesola Samson
Published: January 2023

The main thrust of this paper is to critically examine the place of Information and Communication Technology (ICT) in the effective Teaching and Learning of History in the Nigerian institutions in the 21st century. It argues that the new Information and communication technologies of internet and multimedia which have led to positive impact in the field of education in most developed countries are still at infancy or not used at all in the classroom instruction in most developing countries (Nigeria inclusive). ICTs are potential powerful enabling tools for effective teaching and learning in educational institutions. Thus, the paper argues that the central role of ICT is to provide additional strategies that can be used to address major educational challenges being faced by teachers and students of History Nigerian educational institutions in the 21st century. The paper, therefore, concludes that the appropriate use of ICTs can positively influence and change traditional methods of teaching and learning of History in Nigerian educational institutions, thereby making the teaching and learning of History more concrete. The methodology adopted in this paper is qualitative and analytical utilizing materials from the primary and secondary sources
Leadership of The Principal In Improving Teacher Performance at SDN 12 Rambang
Authors: Darmasita Aini
Published: January 2023

This article aims to reveal about how the principal's leadership in improving teacher performance at the State Elementary School 12 Rambang. This type of research is qualitative research. The subjects of this study were 12 teachers. The data analysis technique used is descriptive analysis technique. Data collection techniques used are interviews and documentation. The findings show that the principal's leadership is good. This can be seen from the principal who motivates teachers, giving warnings to teachers who are less disciplined. And the teacher's performance has been good, judging from the good teacher administration, such as the implementation of learning plans, the use of learning methods according to the material.
Islamic Religious Education Learning Problems: Case Study at SMPN 5 Rejang Lebong
Authors: Wanto Deri
Published: January 2023

The problem raised in this article is the application of schools in overcoming various matters relating to the problem of learning Islamic religious education at SMPN 5 Rejang Lebong. The purpose of this discussion is to find out the problematic forms carried out by schools in overcoming the problems of learning Islamic religious education at SMPN 5 Rejang Lebong and to find out how to overcome the problems of learning Islamic religion. This study uses a qualitative research method with a qualitative descriptive approach. The technique in collecting is using interview, observation and documentation techniques. The final results in this study suggest that, the forms of problems in learning Islamic religious education that occur at SMPN 5 Rejang Lebong, namely, 1) some of the students have not been able to read the Qur'an and also have not been able to do the prayers with the guidance of the prayer. true, 2) students are less interested in religious activities, 3) lack of Islamic religious education learning to adjust the material in learning. While the implementation of the school in overcoming the problem of learning Islamic religious education at SMPN 5 Rejang Lebong are: 1) holding weekly learning to read the Qur'an and practicing prayers, 2) collaborate with the community and parents of students, and 3) organize a Muslim Friday program. So that from several applications in the efforts that have been carried out by the school, it has shown quite good results in overcoming the problem of learning Islamic religious education at SMPN 5 Rejang Lebong
Effects of dance therapy on the rehabilitation of patients with cancer: a systematic review
Authors: Silva Letícia Santos da , Lima Gleissy Mary Souza Santos , Santos Emilly Santana , Cajueiro Catarina Andrade Garcez , Costa Aida Carla Santana de Melo , Santana Licia Santos , Aquino Maria Jane das Virgens , Sousa Davi Santana
Published: January 2023

Introduction: The diagnosis of cancer causes several changes in the lives of human beings, generating impacts on the emotional, physical and psychological state. Fear, stress and sadness usually interfere in quality of life, so dance therapy is a therapeutic method that will serve as an instrument for health rehabilitation, using dance as a means to achieve functional balance and self-esteem of people diagnosed with cancer. Objective: To describe the effectiveness and benefits of dance therapy, in order to emphasize complementary therapies that are important methods during treatment. Material and Methods: Systematic review, in which articles were selected between 2018 and 2021. Published in both Portuguese and English languages in the electronic database as Latin American Caribbean Literature in Sciences of Health (Lilacs), Pubmed, Scientific Electronic Library Online (Scielo) and Physiotherapy Evidence Database (PEDro). Results: The search for data was summarized in a flowchart, in which 546 articles were found based on the combination of descriptors in health science, but after reading, 8 scientific articles were selected divided into three categorical themes; psychological and social. Conclusion: Dance therapy provides physical, psychosocial benefits directly in functional capacity and quality of life during choreography and musical rhythms, influencing functional capacity, self-image, acceptance and especially quality of life.
Enriquecimento ambiental para coelhos domésticos criados em piso na região Sudeste paraense
Authors: Almeida Kalil Izaac de Jesus , Costa Elizabete de Sousa , Oliveira Fernando Gomes de , Oliveira Flavio Gomes de , Pereira Leonardo Vaz , Saldanha Mariana Masseo , Gomes Veruska Dilyanne Silva
Published: January 2023

A cunicultura pode ser viável como atividade geradora de renda extra em pequenas propriedades rurais, uma vez que não requer altos investimentos para o seu desenvolvimento. A região sudeste do Pará é caracterizada por apresentar elevadas temperaturas e umidade relativa do ar, nesse contexto, o uso de material para enriquecimento ambiental pode auxiliar de forma benéfica o bem-estar dos animais e contribuir para minimizar o estresse em ambientes com altas temperaturas. O presente trabalho foi desenvolvido com o objetivo de avaliar o comportamento e interação com materiais utilizados para enriquecimento ambiental de coelhos mantidos em baias coletivas nas condições climáticas do Sudeste do Pará.  O experimento foi desenvolvido no setor de cunicultura da Universidade Federal Rural da Amazônia, campus Parauapebas, PA. Foi avaliado o comportamento e preferência por material para enriquecimento ambiental de quatro coelhos alojados em galpão coletivo. A coleta de dados foi realizada durante três dias, pela manhã e tarde em intervalos de 10 minutos, totalizando 18 horas de observação. A temperatura ambiente variou entre 33ºC e 26,3ºC. O comportamento com maior frequência tanto pela manhã quanto no período da tarde foi o ócio. No período da manhã houve maior interação dos coelhos com a cerâmica e a tarde maior preferência pelo piso de chão batido, estes dois materiais apresentaram as menores temperaturas durante o experimento. Conclui-se que o enriquecimento ambiental pode ser uma estratégia para diminuir o estresse e favorecer as trocas térmicas de calor em criações de coelhos mantidas sobre altas temperaturas.
Análise de manifestação patológicas provenientes do excesso de umidade em quadras poliesportivas padrão FNDE no município de Piripiri-PI
Authors: Lira Fabricio de Sousa , Oliveira Franscisco Arteiro de , Silva Franscisco Robson da , Koseki Hiroshi Cavalcante Medeiros
Published: January 2023

O presente trabalho objetivou o levantamento e identificação de patologias presentes em Quadras Poliesportivas Padrão FNDE (Fundo Nacional de Desenvolvimento da Educação), existentes na cidade de Piripiri-PI. Através do método de inspeção visual, investigaram-se os sintomas e as possíveis causas das manifestações observadas nas edificações em estudo, bem como as medidas mitigadoras necessárias para sanar ou dirimir tais problemas. A falta de manutenção periódica dessas estruturas é um fator preocupante, visto que a qualidade e durabilidade das mesmas possui relação direta com o aparecimento de tais manifestações. Em função desses e de outros fatores, faz-se necessário elaborar um projeto com cautela e empregar materiais de boa qualidade, sempre se preocupando com o surgimento de problemas oriundos de erros de execução, projeto ou uso indiscriminado dos espaços disponíveis. O presente trabalho foi embasado em inspeção visual, registros fotográficos e projetos arquitetônicos de 03 quadras poliesportivas, padrão FNDE. A partir dos dados levantados, constatou-se que as principais patologias observadas estão associadas a problemas de umidade ocasionados principalmente por infiltração de água. Dentre as principais manifestações observadas podem ser citadas a corrosão, fissuras, trincas, rachaduras e mofo na alvenaria. Uma manutenção periódica e especializada é imprescindível para a conservação das edificações, bem como seu uso consciente.
Os tipos de enxertos utilizados no tratamento cirúrgico e efeitos do tratamento fisioterápico em atletas com ruptura do LCA
Authors: Melo Neto Ayrton Soares , Braga Daniel Paulino , Andrade João Pedro Bicalho Borges de , Gomes Danyane Simão
Published: January 2023

As lesões do ligamento cruzado anterior (LCA) e seus tratamentos têm sido muito discutidas, tendo em vista que é uma das injúrias mais frequentes no joelho. Este fato ocorre devido a sua importância biomecânica nesta articulação. Portanto, este estudo teve por finalidade analisar os efeitos dos tratamentos pré e pós-operatórios e sua influência no processo de recuperação do LCA.Tratou-se de revisão integrativa da literatura. A pesquisa foi realizada por meio do acesso online nas bases de dados National Library of Medicine (PubMed MEDLINE), Scientific Electronic Library Online (Scielo), Cochrane Database of Systematic Reviews (CDSR), Google Scholar, Biblioteca Virtual em Saúde (BVS) e EBSCO Information Services, nos meses de setembro a dezembro de 2022. Sobre o processo de recuperação do LCA, é importante ressaltar a reabilitação pré-cirúrgica, buscando restaurar a função neuromuscular e a propriocepção do joelho, além de fortalecer músculos da coxa, como o quadríceps que serão importantes para o seguimento do processo de reabilitação pós-cirúrgica até o retorno ao esporte. Durante a cirurgia são usadas duas principais técnicas como enxerto, técnica de osso-tendão patelar-osso (OTO) e enxerto quádruplo de semitendíneo e grácil (EQSG). Por fim, uma reabilitação pós cirúrgica com um período de pelo menos 8 meses sem retorno aos esportes. Conclui-se que os principais tipos de enxertos utilizados são o OTO e EQSG. O tendão do quadríceps (TO) mostrou-se como opção viável para o tratamento, porém, o autoenxerto OTO é o mais indicado atualmente para o processo cirúrgico de ruptura do LCA.
ВИЗНАЧЕННЯ СПЕЦІАЛЬНОЇ ПРИЗНАЧЕНОСТІ ОКРЕМИХ МОДЕЛЕЙ ВІЙСЬКОВОЇ ВОГНЕПАЛЬНОЇ ЗБРОЇ
Authors: Бондар В. С.
Published: January 2023

У статті сформовано групу основних кваліфікуючих ознак військової вогнепальної зброї спеціальної призначеності. Наявність у конкретного екземпляра зброї хоча б однієї з наступних ознак є достатньою підставою для висновку про спеціальну призначеність цієї зброї: - вказівка виробника (розробника) про спеціальне призначення зброї; - можливість ведення автоматичного вогню (чергами); - компонування зброї за схемою «бул пап» або пістолетного типу; - наявність у конструкції зброї пристрою зниження рівня звуку пострілу, передбачена виробником (розробником) можливість установки таких пристроїв; використання у зброї в якості штатних боєприпасів патронів з механічним запиранням порохових газів у гільзі патрона або патронів спеціального призначення з дозвуковою швидкістю польоту кулі. Констатовано, що сьогодні сформувалися два основних способи вирішення задачі зниження рівня звуку пострілу: 1) безпосереднє застосування ПЗРЗП («глушників» у вигляді різних ствольних насадок) зі спеціальною зброєю; 2) застосування ПЗРЗП замкнутого типу у вигляді спеціальних комплексів, з розширенням та запиранням порохових газів у перемінно-замкненому об’ємі ствола, ствольної насадки або спеціального послабленого дозвукового патрона. Зазначено, що внаслідок великої різноманітності конструктивних схем, не завжди військова вогнепальна зброя спеціальної призначеності має яку-небудь із уже зазначених ознак. Існують й інші конструктивні рішення, які також покращують балістичні й тактичні показники зброї. Причому деякі з них можуть застосовуватись у конструкції як бойової, так і мисливської зброї, а також зброї спеціальної призначеності. Зауважено, що в окремих моделях військової вогнепальної зброї спеціальної призначеності використовуються звичайні патрони, як штатні. Сформульовано висновок про те, що використання спеціальних патронів для проведення пострілів зі зброї спеціальної призначеності є додатковою кваліфікуючою ознакою. Систематизовані закономірності утворення слідів на кулях і гільзах, стріляних із військової вогнепальної зброї спеціальної призначеності, направлені на вдосконалення науково-методичного забезпечення експертних досліджень зброї і створюють необхідні передумови для встановлення фактів застосування такої зброї під час учинення злочинів.
КОНСТИТУЦІЙНО-ПРАВОВЕ РЕГУЛЮВАННЯ МОВНИХ ВІДНОСИН В УКРАЇНІ: ІСТОРІЯ ТА СУЧАСНІСТЬ
Authors: Павшук К. О. , Передерій А. С.
Published: January 2023

Статтю присвячено дослідженню конституційно-правового регулювання відносин у мовній сфері й встановленню його історичних витоків та сучасних тенденцій. Обґрунтовано ідею необхідності ефективного функціонування інституту державної мови, який виступає засадою конституційного ладу України. У статті з’ясовано конституційні засади мовних відносин в Україні, досліджено конституційно-правовий інститут мов, визначено правові засоби, які регулюють відносини в мовній сфері, встановлено сучасні тенденції ефективного конституційно-правового регулювання мовних відносин. У роботі проаналізовані основні етапи трансформації підходів правового регулювання мовних відносин в України, починаючи з радянських часів і до сьогодення. На підставі цього аналізу встановлено кореляцію між належним правовим регулювання мовних відносин та формуванням національної ідентичності як чинника сучасного українського суспільства. На основі системного вивчення конституційно-правового регулювання мовних відносин в Україні визначено, що мовні відносини є безпосереднім об’єктом конституційно-правового регулювання. Однак, зроблено висновок про специфічність природи таких відносин, тому при здійсненні регулювання є необхідність у використанні не всіх правових засобів одночасно, а лише тих, що здатні ефективно врегулювати приватну та публічну сферу спілкування. Також зроблено висновок, що стабільність конституційного ладу України та безпека від потенційних загроз державності через мовне питання залежить від рівня відповідності конституційно-правового регулювання швидким та якісним змінам суспільних відносин. Причому, фундаментом такого регулювання є необхідність встановлення ефективного співвідношення між державною мовою та мовами національних меншин. Запропоновано внести зміни до норми статті 10 Конституції України, де в частині третій прибрати слово «російської» як мови національної меншини.
IMPLEMENTASI ALGORITMA C4.5 DALAM PENENTUAN POLA PEMBELIAN PELANGGAN
Authors: Tukino Tukino , Arnomo Sasa Ani , Hakim Arif Rahman
Published: January 2023

PT Sumber Alfaria Trijaya Tbk atau yang lebih dikenal dengan Alfamart merupakan salah satu perusahaan yang bergerak dibidang ritel yang mana perusahaan ini setiap harinya harus memenuhi kebutuhan konsumen. Selain itu, peletakan produk pada rak yang tidak sesuai dengan kebiasaan konsumen saat membeli produk secara bersamaan dalam satu waktu. Hal ini menyebabkan kekecewaan pada konsumen yang tidak dapat memenuhi kebutuhannya. Maka dari itu perusahaan harus mencermati pola pembelian yang dilakukan oleh konsumen. Untuk mengetahui produk yang dibeli secara bersamaan, menggunakan association rule (aturan asosiasi) yaitu teknik Data Mining  untuk menemukan aturan asosiasi suatu kombinasi item. Proses pencarian asosiasi menggunakan metode algoritma C4.5 yang dapat menghasilkan pengetahuan yang terkandung dalam data transaksi pelanggan, mengenai pola pembelian oleh konsumen. Berdasarkan hasil perhitungan algoritma C4.5 yang memenuhi syarat nilai minimum confidence dan diuji dengan software Tanagra, pada toko Alfamart rule tertinggi yang muncul pada produk Kunci Mas Minyak Goreng Pch 1,8l dan Sedap Mie Kari Special 75G dengan nilai Support 43,3% dan confidence 92,8%, hal ini sama dengan perhitungan manual algoritma C4.5. Maka dapat dilakukan pengaturan tata letak produk secara berdekatan untuk memudahkan keberadaan produk tersebut dan memudahkan dalam menentukan persediaan produk promo yang diinginkan konsumen serta dapat digunakan sebagai pendukung keputusan manajer dalam mengelola aktifitas perusahaan.
THE IMPACT OF REGIONAL ORIGIN INCOME AND ACCOUNTED FUNDS ON THE REGIONAL EXPENDITURE OF THE GOVERNMENT OF THE RIAU ARCHIPELAGO PROVINCE
Authors: Wage Sunarto , Wasiman Wasiman , Rahmat Rahmat , Effendi Syahril , Mardika Nanda Harry
Published: January 2023

This study aims to investigate and analyze the impact of Regional Primary Income (PAD) and balance funds on the regional expenditure of the provincial government of Riau Islands for the period 2009 - 2021, so there are 13 data obtained from the Central Bureau of Statistics (BPS). in the province of Riau Islands. The data analysis technique used in this study is multiple linear regression analysis. Based on the results of the simultaneous hypothesis testing that the Regional Initial Income (PAD) and balance funds together have no effect on the regional expenditure of the provincial government of Riau Islands, with a coefficient of determination test result of 43%.
The roles and therapeutic potentialof mesenchymal stem/stromal cells and their extracellular vesicles in tendinopathies
Authors: Quintero Daniel , Perucca Orfei Carlotta , Kaplan Lee D. , de Girolamo Laura , Best Thomas M. , Kouroupis Dimitrios
Published: January 2023

Tendinopathies encompass a highly prevalent, multi-faceted spectrum of disorders, characterized by activity-related pain, compromised function, and propensity for an extended absence from sport and the workplace. The pathophysiology of tendinopathy continues to evolve. For decades, it has been related primarily to repetitive overload trauma but more recently, the onset of tendinopathy has been attributed to the tissue’s failed attempt to heal after subclinical inflammatory and immune challenges (failed healing model). Conventional tendinopathy management produces only short-term symptomatic relief and often results in incomplete repair or healing leading to compromised tendon function. For this reason, there has been increased effort to develop therapeutics to overcome the tissue’s failed healing response by targeting the cellular metaplasia and pro-inflammatory extra-cellular environment. On this basis, stem cell-based therapies have been proposed as an alternative therapeutic approach designed to modify the course of the various tendon pathologies. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells often referred to as “medicinal signaling cells” due to their immunomodulatory and anti-inflammatory properties that can produce a pro-regenerative microenvironment in pathological tendons. However, the adoption of MSCs into clinical practice has been limited by FDA regulations and perceived risk of adverse events upon infusion in vivo. The introduction of cell-free approaches, such as the extracellular vesicles of MSCs, has encouraged new perspectives for the treatment of tendinopathies, showing promising short-term results. In this article, we review the most recent advances in MSC-based and MSC-derived therapies for tendinopathies. Preclinical and clinical studies are included with comment on future directions of this rapidly developing therapeutic modality, including the importance of understanding tissue loading and its relationship to any treatment regimen.
Synergistic remineralization of enamel white spot lesions using mesoporous bioactive glasses loaded with amorphous calcium phosphate
Authors: Ren Juan , Rao Jianping , Wang He , He Wenjing , Feng Jinnan , Wei Danni , Zhao Bin , Wang Xing , Bian Wei
Published: January 2023

Objectives: The purpose of this study was to create a new delivery system that can synergistically remineralize enamel white spot lesions (WSLs).

Materials and methods: The delivery system (PAA-ACP@aMBG) was prepared by using aminated mesoporous bioactive glasses (aMBG) as the carrier loaded with polyacrylic-stabilized amorphous calcium phosphate (PAA-ACP). The materials were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), inductively coupled plasma–optical emission spectrometry (ICP–OES), and so on. Forty-eight artificial WSLs enamel samples were randomized to four groups: artificial saliva (negative control, NC), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), PAA-ACP@aMBG, and MBG. The effects of demineralization and remineralization of the enamel surface were compared by means of surface microhardness (SMH) measurements, surface color change measurements, fluorescence microscopy (FM), X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM).

Results: There was no significant difference in the surface microhardness recovery rate (SMHRR) or color recovery rate (CRR) among the CPP-ACP group, PAA-ACP@aMBG group and MBG group (P>0.05), but these values were significantly higher than those in the NC group (p < 0.01). FM demonstrated that the remineralization depth in the PAA-ACP@aMBG group was significantly greater than that of the remaining three groups (p < 0.01). SEM analysis indicated that the enamel demineralization marks in the PAA-ACP@aMBG group, CPP-ACP group, and MBG group were obscured by mineral deposition.

Conclusions: PAA-ACP@aMBG showed good mineralization properties, implying its great potential for clinical application.
Aptamer-conjugated gold nanoparticles and their diagnostic and therapeutic roles in cancer
Authors: Deng Guozhen , Zha He , Luo Hongzhi , Zhou Yi
Published: January 2023

The burden of incidence rate and mortality of cancer is increasing rapidly, and the development of precise intervention measures for cancer detection and treatment will help reduce the burden and pain of cancer. At present, the sensitivity and specificity of tumor markers such as CEA and CA-125 used clinically are low, while PET, SPECT, and other imaging diagnoses with high sensitivity possess shortcomings, including long durations to obtain formal reports and the inability to identify the molecular pathological type of cancer. Cancer surgery is limited by stage and easy to recur. Radiotherapy and chemotherapy often cause damage to normal tissues, leading to evident side effects. Aptamers can selectively and exclusively bind to biomarkers and have, therefore, gained attention as ligands to be targeted for cancer detection and treatment. Gold nanoparticles (AuNPs) are considered as promising nano carriers for cancer diagnosis and treatment due to their strong light scattering characteristics, effective biocompatibility, and easy surface modification with targeted agents. The aptamer-gold nanoparticles targeting delivery system developed herein can combine the advantages of aptamers and gold nanoparticles, and shows excellent targeting, high specificity, low immunogenicity, minor side effects, etc., which builds a bridge for cancer markers to be used in early and efficient diagnosis and precise treatment. In this review, we summarize the latest progress in the application of aptamer-modified gold nanoparticles in cancer targeted diagnosis and delivery of therapeutic agents to cancer cells and emphasize the prospects and challenges of transforming these studies into clinical applications.
Treatment of abdominal aortic pseudoaneurysm caused by brucellosis with endovascular aneurysm repair
Authors: Li Xiao , Wang Qilong , Zhang Yang , Sun Xiwei , Yin Hang , Zhang Hua , Luo Sean X. , Wang Zhongying , Yu Qi , Chen Zhiming , Cheng Zhihua
Published: January 2023

Peripheral vascular disease caused by brucellosis is rarely seen around the world; thus, it is easily ignored by patients and doctors, leading to a lack of corresponding screening and delayed comprehensive treatment. Currently, there is no standard or guideline for diagnosing and treating peripheral arterial disease caused by brucellosis. From June 2021 to December 2022, four cases of abdominal aortic pseudoaneurysm caused by brucellosis disease were treated with endovascular aneurysm repair This study reported treatment results as follows and reviewed the incidence, treatment, and prognosis of abdominal aortic pseudoaneurysm caused by brucellosis.
High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding
Authors: Luque Guillermina M. , Schiavi-Ehrenhaus Liza J. , Jabloñski Martina , Balestrini Paula A. , Novero Analia G. , Torres Nicolás I. , Osycka-Salut Claudia E. , Darszon Alberto , Krapf Dario , Buffone Mariano G.
Published: January 2023

The exclusive expression of CatSper in sperm and its critical role in sperm function makes this channel an attractive target for contraception. The strategy of blocking CatSper as a male, non-hormonal contraceptive has not been fully explored due to the lack of robust screening methods to discover novel and specific inhibitors. The reason for this lack of appropriate methodology is the structural and functional complexity of this channel. We have developed a high-throughput method to screen drugs with the capacity to block CatSper in mammalian sperm. The assay is based on removing external free divalent cations by chelation, inducing CatSper to efficiently conduct monovalent cations. Since Na+ is highly concentrated in the extracellular milieu, a sudden influx depolarizes the cell. Using CatSper1 KO sperm we demonstrated that this depolarization depends on CatSper function. A membrane potential (Em) assay was combined with fluorescent cell barcoding (FCB), enabling higher throughput flow cytometry based on unique fluorescent signatures of different sperm samples. These differentially labeled samples incubated in distinct experimental conditions can be combined into one tube for simultaneous acquisition. In this way, acquisition times are highly reduced, which is essential to perform larger screening experiments for drug discovery using live cells. Altogether, a simple strategy for assessing CatSper was validated, and this assay was used to develop a high-throughput drug screening for new CatSper blockers.
Prospective clinical study of retinal microvascular alteration after ICL implantation
Authors: Tang Chuhao , Zhang Yu , Sun Tong , Xie Jianyang , Liu Yiyun , Liu Rongjun , Sun Zhengze , Qi Hong
Published: January 2023

Purpose: To evaluate the retinal microvascular alteration after implantable collamer lens (ICL) implantation in moderate to high myopia patients using quantitative optical coherence tomography angiography (OCTA).

Methods: This prospective cohort study included 50 eyes of 25 patients with preoperative spherical equivalent ≥ −3.00 D. Patients underwent bilateral ICL implantation at the Department of Ophthalmology, Peking University Third Hospital, from November 2018 to July 2019. OCTA was used to image the superficial and deep retinal capillary plexuses before ICL implantation surgery and at 3 months follow-up.

Results: There was no significant difference in the microvascular density within each annular zone and all quadrantal zones of the superficial and deep layers found in myopia patients before and after ICL surgery.

Conclusion: Levels of microvascular density in retinal capillary plexuses were stable, as detected by the OCTA, showing the high security of ICL implantation, which would not leave adverse effects on retinal microvasculature in myopia patients.
Bradycardia unresponded to atropin testing was successfully treated with therapeutic plasma exchange in a patient with severe COVID-19 complicated by Guillain-Barré syndrome: A case report
Authors: Duong-Quy Sy , Huynh-Truong-Anh Duc , Tran-Xuan Quynh , Nguyen-Quang Tien , Nguyen-Thi-Kim Thanh , Nguyen-Chi Thanh , Tran-Ngoc-Anh Thuy , Nguyen-Van-Hoai Nam , Do-Thi-Thu Mai , Tang-Thi-Thao Tram , Bui-Diem Khue , Hoang-Anh Tien , Nguyen-Ngoc-Phuong Thu , Nguyen-Nhu Vinh
Published: January 2023

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been an alarming situation worldwide for the past 2 years. The symptoms of coronavirus disease 2019 (COVID-19) are not only confined to the respiratory system but also affect a multitude of organ systems. Bradycardia associated with Guillain-Barré syndrome (GBS) is a rare autonomic and peripheral neurological complication of COVID-19. In this case report, we present the case of a 26-year-old man diagnosed with bradycardia associated with GBS after contracting COVID-19. Initially, this patient had the classical symptoms of COVID-19 and was hospitalized in the intensive care unit (ICU) for acute respiratory distress syndrome (ARDS). Then, he developed weakness in the lower extremities, diminished tendon reflexes, a loss of sensation without sphincter muscle disorders, and bradycardia. His bradycardia did not respond to atropine. The patient was treated concurrently with a high-flow nasal cannula, systemic corticosteroids, anticoagulation, and therapeutic plasma exchange (TPE) for COVID-19-induced ARDS, bradycardia, and GBS. His ARDS and bradycardia improved after the first cycle of TPE and medical treatment. After three cycles of TPE, the patient progressively recovered his muscle strength in the lower limbs and regained peripheral sensation. He was discharged from the hospital in stable condition after 4 weeks of hospitalization and was followed up after 6 months for cardiorespiratory and neurological complications. This case report elucidates the potential difficulties and challenges that physicians may encounter in diagnosing and treating COVID-19-induced bradycardia and GBS during the pandemic outbreak. However, the patient outcomes with the treatment combining the conventional treatment with therapeutic plasma exchange seem to be optimistic.
Prediction of myocardial ischemia in coronary heart disease patients using a CCTA–Based radiomic nomogram
Authors: Yang You-Chang , Dou Yang , Wang Zhi-Wei , Yin Ruo-Han , Pan Chang-Jie , Duan Shao-Feng , Tang Xiao-Qiang
Published: January 2023

Objective

The present study aimed to predict myocardial ischemia in coronary heart disease (CHD) patients based on the radiologic features of coronary computed tomography angiography (CCTA) combined with clinical factors.

Methods

The imaging and clinical data of 110 patients who underwent CCTA scan before DSA or FFR examination in Changzhou Second People’s Hospital, Nanjing Medical University (90 patients), and The First Affiliated Hospital of Soochow University (20 patients) from March 2018 to January 2022 were retrospectively analyzed. According to the digital subtraction angiography (DSA) and fractional flow reserve (FFR) results, all patients were assigned to myocardial ischemia (n = 58) and normal myocardial blood supply (n = 52) groups. All patients were further categorized into training (n = 64) and internal validation (n = 26) sets at a ratio of 7:3, and the patients from second site were used as external validation. Clinical indicators of patients were collected, the left ventricular myocardium were segmented from CCTA images using CQK software, and the radiomics features were extracted using pyradiomics software. Independent prediction models and combined prediction models were established. The predictive performance of the model was assessed by calibration curve analysis, receiver operating characteristic (ROC) curve and decision curve analysis.

Results

The combined model consisted of one important clinical factor and eight selected radiomic features. The area under the ROC curve (AUC) of radiomic model was 0.826 in training set, and 0.744 in the internal validation set. For the combined model, the AUCs were 0.873, 0.810, 0.800 in the training, internal validation, and external validation sets, respectively. The calibration curves demonstrated that the probability of myocardial ischemia predicted by the combined model was in good agreement with the observed values in both training and validation sets. The decision curve was within the threshold range of 0.1–1, and the clinical value of nomogram was higher than that of clinical model.

Conclusion

The radiomic characteristics of CCTA combined with clinical factors have a good clinical value in predicting myocardial ischemia in CHD patients.
Dynamic evolution of changbaishan volcanism in Northeast China illuminated by machine learning
Authors: Zhao Yong , Zhang Yigang , Ni Dongdong
Published: January 2023

Previous studies propose that there is a mantle upwelling that generated the Cenozoic basalts in Changbaishan. However, the dominant source and mechanism of the mantle upwelling remains highly debated. Here we apply machine learning algorithms of Random Forest and Deep Neural Network to train models using global island arc and ocean island basalts data. The trained models predict that Changbaishan basalts are highly influenced by slab-derived fluid. More importantly, the fluid effect decreases with no (87Sr/86Sr)0 and εNd(t) changes between 5 Ma and 1 Ma, then enhances with increasing εNd(t) and decreasing (87Sr/86Sr)0 after 1 Ma. We propose that a gap opened at about 5 Ma and the hot sub-slab oceanic asthenosphere rose through the gap after 1 Ma, generating the basalts enriched in fluid mobile elements and with the addition of depleted mantle component derived from the sub-slab oceanic asthenosphere.
Carbon mobilization in response to the 2021 Mw 7.4 Maduo earthquake: Constraints from carbon isotope systematics of subsurface fluids
Authors: Liu Yi , Liu Wei , Zhou Xiaocheng , Zhong Jun , Zhang Maoliang , Xu Sheng
Published: January 2023

Active fault zones provide favorable channels for the discharge of carbon-bearing fluids from Earth’s interior. Earthquakes, as a common fault-related dynamic process, can disturb the circulation of subsurface fluids and their interactions with country rocks and sediments on short timescales, which may cause changes in carbon mobilization processes and carbon sources of the discharged fluids. However, quantitative research on earthquake-induced changes in carbon mobilization at deep and shallow levels remains lacking. Here, we present a quantitative study on stable carbon isotopes (δ13C) and radiocarbon values (Δ14C) of dissolved inorganic carbon (DIC) in subsurface fluid samples from the surface rupture zone formed by the Mw 7.4 Maduo earthquake (22 May 2021) and the East Kunlun fault, NE Tibetan Plateau. Our results show that δ13CDIC values vary from –11.6‰ to 0.1‰, while Δ13CDIC values have a range of –980‰ to –46‰. Using a mass balance model based on δ13CDIC and DIC concentrations, we calculated the proportions of source components involved in DIC, including organic carbon, carbonates, and deeply-sourced carbon. On average, waters discharging from the surface rupture zone have higher inputs from organic carbon (28.1%) than those from the East Kunlun fault (18.6%), with the latter showing higher deeply-sourced carbon contributions (45.7% vs. 30.7%). This is consistent with the lower average Δ14CDIC value (–544‰) observed from the East Kunlun fault, suggesting more inputs from carbon source components that are devoid of 14C (i.e., deeply-sourced carbon and carbonates). These findings indicate that seismic events can significantly affect the carbon mobilization processes at variable depths, especially the shallow soil organic carbon in the case of the 2021 Maduo earthquake. The potential effects of earthquake-induced changes in carbon mobilization processes should be taken into account in the modeling of tectonic carbon dioxide degassing and carbon cycle on longer timescales.
Experimental method and application of the slurry “diffusion-bleeding-seepage” of isolated overburden grout injection
Authors: Wang Chaochao , Xu Jialin , Xuan Dayang , Li Jian
Published: January 2023

Isolated overburden grout injection (IOGI) is a green mining method to control surface subsidence. Slurry water significantly influences grouting effectiveness and mining safety. This study establishes a three-dimensional visualization experimental system for slurry “diffusion-bleeding-seepage” to investigate the seepage law of slurry water. The system is composed primarily of a transparent box (1.2 m × 0.5 m × 0.25 m) and support, with the solid–liquid coupling seepage similar material and modules for mining, grouting injection, slurry pressure and displacement monitoring, overburden saturation monitoring, and water leakage monitoring of the working face. A similar material with good permeability and non-disintegration is obtained by hydrophilic, water absorption, and permeability tests. Grouting and mining are simulated by pulling acrylic sheets and pumping slurry. With the fly ash slurry entering the injection layer, the slurry undergoes water–cement separation, and the water bleeding is formed to seep into the similar material. The volumetric water content of the similar material is obtained by arranging multiple groups of volumetric water content sensors into the similar material. The corresponding saturation is obtained by theoretical calculation. The experimental system is used to simulate the seepage of slurry water in a high initial saturation overburden, and the characteristics of injection slurry diffusion and water bleeding are obtained. The profile distribution of seepage of slurry water is found to possess a semi-elliptical shape. Under the condition of high initial saturation, slurry water appears in the working face. The system provides a convenient method for further research of IOGI slurry water seepage.
Geochemistry, zircon U-Pb age and Hf isotope for the Huatugou granitoid in western Qaidam: Petrogenesis and tectonic implications
Authors: Teng Xia , Zhang Jianxin , Mao Xiaohong , Wu Yawei , Guo Qi
Published: January 2023

The Qaidam Precambrian block is located in the northeastern Tibetan Plateau and was intruded by numerous Ordovician-Devonian granitoids during and after the closure of the Proto-Tethys Ocean. In the past 20 years, the granitoids within early Paleozoic subduction-collision belts have been investigated in detail. However, the granitoids intruding the inner part of the Qaidam block, bearing the tectonic significance for the whole block, still need to be understood. This study presents new whole-rock geochemical and zircon U-Pb-Hf isotopic data for the Huatugou granitoids in the Qaidam Precambrian block. The investigated granitoids include granodiorite, monzogranite, biotite granite, and muscovite granite, which intruded the Precambrian basements during 451–400 Ma. The granodiorites (451 ± 6 Ma) display adakitic geochemical features and syn-tectonic textures, and their magmas were generated by the partial melting of the lower mafic crust within a thickened continent. The muscovite granites (410 ± 6 Ma), with negative zircon εHf(t) values of −14.5 to −10.4, were crystallized from fractionated S-type magmas, which were derived from the partial melting of ancient crustal materials. The biotite granite (410 ± 3 Ma) and monzogranites (400 ± 4 Ma) are high-temperature A2-type granites. The biotite granite displays positive zircon εHf(t) values of +1.7 to +5.6. Its magma was generated by the high-temperature partial melting of juvenile crustal rocks in a thinned lower crust. The monzogranites exhibit higher SiO2 contents and lower εHf(t) values, and their magmas were derived from the same source but underwent assimilation and fractional crystallization during emplacement. From the thickened to the thinned continent during 451–410 Ma, the western Qaidam block experienced a tectonic transition from compression to extension. Combined with regional geological data, this study suggests that the Qaidam block consisted of the thickened continental crust during subduction processes until the detachment of the subducted slab during the continental collision. The regional extension of the Qaidam block commenced at ∼420 Ma, soon after the exhumation of ultrahigh-pressure metamorphic rocks within the northern Qaidam subduction-collision complex belt.
Influence of mud intercalation on the stability of seepage flow in tailings dams
Authors: Li Hongru , Yang Min , Dang Tong
Published: January 2023

Layers with low permeability are an important factor affecting the stability of tailings dams. In order to analyze the influence of the weak permeability interlayer on the seepage stability of the dam, this paper establishes a numerical analysis model of the tailings dam with the weak permeability interlayer. The seepage stability of the dam slope is calculated and analyzed for the location, thickness, number, and intervals of mud intercalation on the seepage stability of the dam slope. The weakly permeable interlayers at different locations form different saturation lines in the dam. When the weak permeability interlayer is located in the middle of the dam, the height of the saturation line is the highest, and the corresponding stability safety factor is the smallest. When the weak permeability interlayer moves up or down, the buried depth of the saturation line increases, and the safety factor increases. However, when the weak permeability interlayer is located above the drainage prism, the overall slip along the weak permeability interlayer is incidental to the increase in dam height. When the thickness of the weak permeability interlayer changes, the safety factor of the dam has a positively high significance related to the leakage. When the location of the weak permeability interlayer changes, the safety factor of the dam has no obvious correlation with the leakage. With the increase of the thickness and number of weak pemeability interlayer and the decrease of the intervals of mud intercalation, the height of the saturation line gradually increases, and the safety factor of the dam gradually decreases. Through the location of the sliding surface, it is found that the sliding surface eventually destabilizes through the lowest low-permeability interlayer.
Multiscale mechanical characterizations of ultrafine tailings mixed with incineration slag
Authors: Jing Xiaofei , Wu Shangwei , Qin Jia , Li Xiaoshuang , Liu Xiaohua , Zhang Yuanzhen , Mao Jingxin , Nie Wen
Published: January 2023

Adding a type of municipal solid waste (incinerator slag) into ultrafine tailings can effectively enhance the mechanical properties of tailings. With an aim to study the macro- and micro-mechanical properties of the tailings slag mixture (TSM), the strength parameters (internal friction angle, cohesion) and micro-mechanical properties with different slag contents were analyzed by geotechnical experiments and particle flow simulations, respectively. The macroscale experimental results demonstrated that the strength parameters of TSM were much higher than that of tailings. Strength parameters also showed non-linear-rising trends with increasing slag content. For the slag content of 40%, the maximum cohesiveness of TSM was determined at 65.2 kPa, and the corresponding friction angle was 39.9°C. Furthermore, the Particle Flow Code (PFC) micro-simulation software was used to analyze the micro-mechanical characteristics of the TSM at different slag contents. The microscale simulation outcomes indicated that the particle transport, particularly in their moving directions, became increasingly chaotic with an increase in the slag content; also, the slag particles significantly impacted the shear processing zone of the TSM. These experimental and numerical results brought more scientific insights into the shear failure mechanism of TSM.
Dense-array adjoint tomography reveals lithospheric delamination and asthenosphere upwelling beneath the western Yangtze Craton
Authors: Xiao Zhuo , Sun Xinlei , Yang Jianfeng , Gao Yuan
Published: January 2023

The eastern Tibetan Plateau has attracted widespread attention due to its complex topography and strong seismicity. However, the mechanism controlling the growth of this margin remains enigmatic. Here, we present detailed upper mantle structures of the easternmost and northeastern Tibetan Plateau from dense-array adjoint waveform tomography. The seismic images show mushroom-shaped low-velocity zones atop at the uppermost mantle of the eastern margin of the Tibetan Plateau and slab-shaped high-velocity bodies preserved beneath both the eastern Himalayan syntaxis and the western Sichuan basin. The seismic features suggest asthenosphere upwelling and lithospheric delamination beneath the western Yangtze Craton, which might be induced by the retreat of the subducted Indian Plate. Our study shows that the mantle dynamics of the western Yangtze Craton have played an important role in the Tibetan Plateau growth and suggests that the Yangtze Craton might be reconstructed by ongoing continental collision.