bluuebunny/crossref_metadata_2025 huggingface dataset:
SU4312 Represses Glioma Progression by Inhibiting YAP and Inducing Sensitization to the Effect of Temozolomide
Authors: Wang Xu , Zhou Yi , Wang Yan , Wang Xiang , Zhang Yu , Mao Yufei , Zhang Long , Qi Ji , Zhang Yining , Lyu Feng , Gu Linbo , Yu Rutong , Zhou Xiuping
Published: August 2022

SU4312, initially designed as a multi-target tyrosine kinase inhibitor, is consequently reported to inhibit tumor angiogenesis by blocking VEGFR. However, although SU4312 can penetrate the brain–blood barrier, its potential to inhibit glioma growth is unknown. In this study, we report that SU4312 inhibited glioma cell proliferation and down-regulated yes-associated protein (YAP), the key effector of the hippo pathway. The exogenous over-expression of YAP partially restored the inhibitory effect of SU4312 on glioma progression. Interestingly, SU4312 sensitized the antitumor effect of temozolomide, both in vitro and in vivo. Moreover, SU4312 decreased the M2tumor-associated macrophages and enhanced anti-tumor immunity by down-regulating the YAP-CCL2 axis. In conclusion, our results suggest that SU4312 represses glioma progression by down-regulating YAP transcription and consequently CCL2 secretion. SU4312 may be synergistic with temozolomide for glioma treatment.
European Prevalence of Polypoidal Choroidal Vasculopathy: A Systematic Review, Meta-Analysis, and Forecasting Study
Authors: van Dijk Elon , Holtz Jeppe , Sirks Marc , Larsson Janni , Diederen Roselie , Schlingemann Reinier , Boon Camiel , Subhi Yousif
Published: August 2022

The purpose of this study was to provide an estimate of the number of current and future patients with polypoidal choroidal vasculopathy (PCV) in Europe. We systematically searched 11 literature databases on 18 May 2022 for studies on the prevalence of PCV among a consecutive and representative group of patients with suspected neovascular age-related macular degeneration (AMD). Prevalence of PCV in patients with suspected neovascular AMD was summarized and included in a prevalence meta-analysis. We then used current population data and population forecasts by Eurostat and the Office for National Statistics to determine current and future number of patients with neovascular AMD in Europe. Then, we calculated the number of patients with PCV with our calculated estimate of the prevalence of PCV among Europeans suspected with neovascular AMD. A total of five eligible studies were identified which included a total of 1359 patients. All these studies used the gold standard of indocyanine green angiography as a routine part of their diagnostic approach. Among patients undergoing detailed retinal examination for suspected neovascular AMD, our meta-analysis calculated the prevalence of PCV to be 8.3% (95% confidence interval: 6.8–9.8%). Our population estimates find that a total of 217,404 patients with PCV exist in Europe in the year 2022, which constitutes 0.04% of the entire population of Europe. This number is estimated to increase to 287,517 patients in the year 2040. Our estimates are important for different healthcare stakeholders, especially when planning and allocating expensive resources.
Efficacy and Safety of Empagliflozin in Type 2 Diabetes Mellitus Saudi Patients as Add-On to Antidiabetic Therapy: A Prospective, Open-Label, Observational Study
Authors: Althobaiti Fahad M. , Alsanosi Safaa M. , Falemban Alaa H. , Alzahrani Abdullah R. , Fataha Salma A. , Salih Sara O. , Alrumaih Ali M. , Alotaibi Khalid N. , Althobaiti Hazim M. , Al-Ghamdi Saeed S. , Ayoub Nahla
Published: August 2022

The Saudi Food and Drug Authority (SFDA) approved sodium-glucose cotransporter-2 (SGLT2) inhibitors in 2018. The efficacy and safety of empagliflozin (EMPA) have been confirmed in the U.S., Europe, and Japan for patients with type 2 diabetes mellitus (T2DM); however, analogous evidence is lacking for Saudi T2DM patients. Therefore, the current study aimed to assess the efficacy and safety of EMPA in Saudi patients (n = 256) with T2DM. This is a 12-week prospective, open-label, observational study. Adult Saudi patients with T2DM who had not been treated with EMPA before enrolment were eligible. The exclusion criteria included T2DM patients less than 18 years of age, adults with type one diabetes, pregnant women, paediatric population. The results related to efficacy included a significant decrease in haemoglobin A1c (HbA1c) (adjusted mean difference −0.93% [95% confidence interval (CI) −0.32, −1.54]), significant improvements in fasting plasma glucose (FPG) (−2.28 mmol/L [95% CI −2.81, −1.75]), and a reduction in body weight (−0.874 kg [95% CI −4.36, −6.10]) following the administration of 25 mg of EMPA once daily as an add-on to ongoing antidiabetic therapy after 12 weeks. The primary safety endpoints were the change in the mean blood pressure (BP) values, which indicated significantly reduced systolic and diastolic BP (−3.85 mmHg [95% CI −6.81, −0.88] and −0.06 mmHg [95% CI −0.81, −0.88], respectively) and pulse rate (−1.18 [95% CI −0.79, −3.15]). In addition, kidney function was improved, with a significant reduction in the urine albumin/creatinine ratio (UACR) (−1.76 mg/g [95% CI −1.07, −34.25]) and a significant increase in the estimated glomerular filtration rate (eGFR) (3.54 mL/min/1.73 m2 [95% CI 2.78, 9.87]). Furthermore, EMPA reduced aminotransferases (ALT) in a pattern (reduction in ALT > AST). The adjusted mean difference in the change in ALT was −2.36 U/L [95% CI −1.031, −3.69], while it was −1.26 U/L [95% CI −0.3811, −2.357] for AST and −1.98 U/L [95% CI −0.44, −3.49] for GGT. Moreover, in the EMPA group, serum high-density lipoprotein (HDL) significantly increased (0.29 mmol/L [95% CI 0.74, 0.15]), whereas a nonsignificant increase was seen in low-density lipoprotein (LDL) (0.01 mmol/L [95% CI 0.19, 0.18]) along with a significant reduction in plasma triglyceride (TG) levels (−0.43 mmol/L [95% CI −0.31, −1.17]). Empagliflozin once daily is an efficacious and tolerable strategy for treating Saudi patients with insufficiently controlled T2DM as an add-on to ongoing antidiabetic therapy.
Available-Maneuvering-Margins-Based Ship Collision Alert System
Authors: Du Lei , Valdez Banda Osiris A. , Sui Zhongyi
Published: August 2022

The timing of a ship taking evasive maneuvers is crucial for the success of collision avoidance, which is affected by the perceived risk by the navigator. Therefore, we propose a collision alert system (CAS) based on the perceived risk by the navigator to trigger a ship’s evasive maneuvers in a timely manner to avoid close-quarters situations. The available maneuvering margins (AMM) with ship stability guarantees are selected as a proxy to reflect the perceived risk of a navigator; hence, the proposed CAS is referred to as an AMM-based CAS. Considering the dynamic nature of ship operations, the non-linear velocity obstacle method is utilized to identify the presence of collision risk to further activate this AMM-based CAS. The AMM of a ship are measured based on ship maneuverability and stability models, and the degree to which they violate the risk-perception-based ship domain determines the level of collision alert. Several typical encounter scenarios are selected from AIS data to demonstrate the feasibility of this AMM-based CAS. The promising results suggest that this proposed AMM-based CAS is applicable in both ship pair encounter and multi-vessel encounter scenarios. Collision risk can be accurately detected, and then a collision alert consistent with the risk severity is issued. This proposed AMM-based CAS has the potential to assist autonomous ships in understanding the risk level of the encounter situation and determining the timing for evasive maneuvers. The advantages and limitation of this proposed method are discussed.
Research on Ship Collision Probability Model Based on Monte Carlo Simulation and Bi-LSTM
Authors: Vukša Srđan , Vidan Pero , Bukljaš Mihaela , Pavić Stjepan
Published: August 2022

The efficiency and safety of maritime traffic in a given area can be measured by analyzing traffic density and ship collision probability. Maritime traffic density is the number of ships passing through a given area in a given period of time. It can be measured using vessel tracking systems, such as the Automatic Identification System (AIS). The information provided by AIS is real-time data designed to improve maritime safety. However, the AIS data can also be used for scientific research purposes to improve maritime safety by developing predictive models for collisions in a research area. This article proposes a ship collision probability estimation model based on Monte Carlo simulation (MC) and bidirectional long short-term memory neural network (Bi-LSTM) for the maritime region of Split. The proposed model includes the processing of AIS data, the verification of AIS data, the determination of ports and ship routes, MC and the collision probability, the Bi-LSTM learning process based on MC, the ship collision probability for new or existing routes, and the traffic density. The results of MC, i.e., traffic/vessel route and density, and collision probability for the study area can be used for Bi-LSTM training with the aim of estimating ship collision probability. This article presents the first part of research that includes MC in detail, followed by a preliminary result based on one day of processed AIS data used to simulate MC and propose a model architecture that implements Bi-LSTM for ship collision probability estimation.
Progressive Additive Benefits of Prehabilitation and Subsequent Bariatric Surgery on Cardiac Autonomic Regulation as Assessed by Means of a Simple Unitary Composite Index: Preliminary Data from an Observational Study
Authors: Giovanelli Luca , Palombo Carlo , Pina Matteo , Facchetti Simone , Malacarne Mara , Pagani Massimo , Nannipieri Monica , Berta Rossana , Lucini Daniela
Published: August 2022

Obesity is associated with an increased risk of several chronic comorbidities, which may also be determined by dysfunctional autonomic nervous system (ANS). The influence of bariatric surgery (BS) on ANS balance was explored in previous studies, but with high heterogeneity in both the assessment timing and methods employed. In the present observational study, we applied a clinical protocol which considers two subsequent phases. Twenty-nine non-diabetic obese subjects were studied at baseline (T0), after one month of lifestyle modification (prehabilitation) (phase 1-T1), and after eight months following BS (phase 2-T2). ANS regulation was assessed across the three study epochs by means of ANSI, a single composite percent-ranked proxy of autonomic balance, being free of gender and age bias, economical and simple to apply in a clinical setting. The aim of the present study was to investigate the effects of the clinical protocol based on prehabilitation and subsequent BS on the ANS regulation by means of ANSI. Potential intertwined correlations with metabolic parameters were also investigated. Notably, we observed a progressive improvement in ANS control, even by employing ANSI. Moreover, the reduction in the markers of sympathetic overactivity was found to significantly correlate with the amelioration in some metabolic parameters (fasting glucose, insulin levels, and waist circumference), as well as in stress and tiredness perception. In conclusion, this study provides convincing evidence that a unitary proxy of cardiac autonomic regulation (CAR) may reflect the progressive improvement in autonomic regulation following behavioral and surgical interventions in obese patients. Intriguingly, this might contribute to reducing cardiovascular and metabolic risk.
Corporate Financial Strategy in an Emerging Market: Evidence from Indonesia
Authors: Alghifari Erik Syawal , Hermawan Atang , Gunardi Ardi , Rahayu Agus , Wibowo Lili Adi
Published: August 2022

This paper focuses on strategic corporate financial decisions related to capital structure to increased firm value, moderated by the COVID-19 pandemic under MM theory, trade-off theory, and pecking order theory. The analytical method used is panel data analysis, with observations of 1828 non-financial companies on the Indonesia Stock Exchange from the years 2019 to 2021. The results show that there is an effect of capital structure on firm value in a positive direction, and the moderating role of the COVID-19 pandemic weakens the effect of capital structure on firm value. The findings show that capital structure only has a significant effect on firm value for the debt-dominant group, but not for the equity-dominant group. The moderating effect of the COVID-19 pandemic affects firm value for the debt-dominant group, but not for the equity-dominant group.
Determining the Cadmium Accumulation in Maize (Zea mays L.) and Soil Influenced by Phosphoric Fertilizers in Two Different Textured Soils
Authors: Suleman Muhammad , Ashraf Muhammad , Raza Qurat-Ul-Ain , Bashir Muhammad Amjad , Rahman Shafeeq Ur , Aon Muhammad , Ali Saba , Shahzad Sher Muhammad , Khalid Muhammad Usman , Raza Hafiz Muhammad Ali , Rehim Abdur , Du Zhenjie
Published: August 2022

Non-nutritive metals, especially cadmium (Cd), are present in P fertilizers; the long-term application of these P fertilizers leads to Cd build-up in the soil. The current study aims to evaluate the impacts of P sources and rates on the growth of maize (Zea mays L.) and the bioavailability of Cd. Twelve treatments including rock phosphate 4 g kg−1 (RP1); 8 g kg−1 (RP2); 12 g kg−1 (RP3)); single super phosphate 333 mg kg−1 (SSP1); 444 mg kg−1 (SSP2); 555 mg kg−1 (SSP3); di-ammonium phosphate 130 mg kg−1 (DAP1); 174 mg kg−1 (DAP2); 218 mg kg−1 (DAP3); mono-ammonium phosphate 115 mg kg−1 (MAP1); 154 mg kg−1 (MAP2); 193 mg kg−1 (MAP3) in two soil textures (sandy and clayey) were assessed. Results revealed that all P sources significantly influenced the plant growth and yield characteristics of maize (p ≤ 0.05). In both soil textures, P in soil and plant, plant growth and yield characteristics were maximized by MAP and DAP. Cadmium build-up in soil and uptake was also significantly (p ≤ 0.05) affected by P sources, levels, and soil texture. It was observed that Cd build-up in soil and uptake by plants boosted with increasing P levels. Maximum Cd concentration in plant root and shoot was found with SSP3, and its concentration in soil increased with MAP3, whereas the concentration was higher in sandy texture. The study concludes that type of P fertilizer should be determined based on texture and human consumption of the crop to avoid Cd toxicity.
Evaluating the Effects of Renewable Energy Consumption on Carbon Emissions of China’s Provinces: Based on Spatial Durbin Model
Authors: Sun Yang , Du Mengna , Wu Leying , Li Changzhe , Chen Yulong
Published: August 2022

Renewable energy consumption is considered as the main form of energy consumption in the future. The carbon emissions produced by renewable energy can be approximately ignored, and renewable energy is essential for regional sustainable development. In this study, we used the Durbin model with panel data to explore the spatial dependence between renewable energy consumption the and carbon emissions of China’s 30 provinces from 1997 to 2017. The results show that: (1) there is a negative spatial correlation between renewable energy consumption and carbon emissions, and “High-Low” areas are mainly concentrated in southern provinces in 1997–2011; (2) the center of gravity of renewable energy consumption moves southwest, which is consistent with the center of gravity of carbon emissions; (3) renewable energy consumption has a significant inhibitory effect on carbon emissions of a local region, but the spatial spillover effect is not significant. Specifically, a 1% increase in renewable energy consumption in a region will reduce carbon emissions by 0.05%. Finally, on the basis of this study, it was proposed to give full play to the advantages of renewable energy in the western region, and further accelerate the development of the renewable energy industry.
The Synthesis and Assembly of a Truncated Cyanophage Genome and Its Expression in a Heterogenous Host
Authors: Liu Shujing , Feng Jia , Sun Tao , Xu Bonan , Zhang Jiabao , Li Guorui , Zhou Jianting , Jiang Jianlan
Published: August 2022

Cyanophages play an important role in regulating the dynamics of cyanobacteria communities in the hydrosphere, representing a promising biological control strategy for cyanobacterial blooms. Nevertheless, most cyanophages are host-specific, making it difficult to control blooming cyanobacteria via single or multiple cyanophages. In order to address the issue, we explore the interaction between cyanophages and their heterologous hosts, with the aim of revealing the principles of designing and constructing an artificial cyanophage genome towards multiple cyanobacterial hosts. In the present study, we use synthetic biological approaches to assess the impact of introducing a fragment of cyanophage genome into a heterologous cyanobacterium under a variety of environmental conditions. Based on a natural cyanophage A-4L genome (41,750 bp), a truncated cyanophage genome Syn-A-4-8 is synthesized and assembled in Saccharomyces cerevisiae. We found that a 351–15,930 bp area of the A-4L genome has a fragment that is lethal to Escherichia coli during the process of attempting to assemble the full-length A-4L genome. Syn-A-4-8 was successfully introduced into E. coli and then transferred into the model cyanobacterium Synechococcus elongatus PCC 7942 (Syn7942) via conjugation. Although no significant phenotypes of Syn7942 carrying Syn-A-4-8 (LS-02) could be observed under normal conditions, its growth exhibited a prolonged lag phase compared to that of the control strain under 290-millimolar NaCl stress. Finally, the mechanisms of altered salt tolerance in LS-02 were revealed through comparative transcriptomics, and ORF25 and ORF26 on Syn-A-4-8 turned out to be the key genes causing the phenotype. Our research represents an important attempt in designing artificial cyanophages towards multiple hosts, and offers new future insights into the control of cyanobacterial blooms.
A Novel Green Logistics Technique for Planning Merchandise Deliveries: A Case Study
Authors: Hernández-Mejía Carlos , Torres-Muñoz Delia , Inzunza-González Everardo , Sánchez-López Carlos , García-Guerrero Enrique Efrén
Published: August 2022

Background: The health crisis due to COVID-19 has changed the habits of social coexistence and therefore has had a significant impact on several economic sectors, including logistics. Nowadays, this sector faces one of the most difficult challenges in history and hence has carried out innovative strategies to adjust to the new normal and guarantee the permanence of the supply chain. In this paper, a novel green logistics technique for planning merchandise deliveries is introduced herein. Methods: This technique is based on a modified version of the Resistive Grid Path Planning Methodology (RGPPM) to establish a path through georeferential locations for delivering merchandise to customers. To do this, multi-connected resistive grids are based at customer locations for searching the path connecting the positions through electronic circuit analysis techniques. Results: Experimental results reveal that the proposed method can find a minimum spanning tree that connects all hand-over points by a continuous path. This finding has represented a reduction of around 45% in the length of the path with respect to the longest path. Conclusions: Finally, this technique is capable of addressing different optimization strategies, locating a merchandise distribution center and exploring environmental standards to reduce fuel consumption.
Applied Mathematics for 5th Generation (5G) and beyond Communication Systems
Authors: Ali Rashid , Kim Hyung Seok
Published: August 2022

Mobile communication systems have a great importance in today’s world [...]
PyVaporation: A Python Package for Studying and Modelling Pervaporation Processes
Authors: Sapegin Denis Andzheevich , Chekmachev Aleksei Viktorovich
Published: August 2022

PyVaporation—a freely available Python package with an open-source code for modelling and studying pervaporation processes—is introduced. The theoretical background of the solution, its applicability and limitations are discussed. The usability of the package is evaluated using various examples of working with and modelling experimental data. A general equation for the representation of a component’s permeance as a function of feed composition, temperature and initial feed composition is proposed and implemented in the developed package. The suggested general permeance equation may be used for the description of an extremal character of permeance as a function of process temperature and feed composition, allowing the description of processes with a high degree of non-ideality. The application of the package allowed modelling experimental points of various sets of hydrophilic pervaporation data and data on membrane performance from independent sources with a relative root mean square deviation of not more than 9% for flux and not more than 5% for a separated mixture concentration. The application of the facilitated parameter approach allowed the prediction of the components’ permeance as a function of feed concentration at various initial feed concentrations with a relative root mean square error of 3–26%. The package was proven useful for modelling isothermal and adiabatic time and length-dependent pervaporation processes. The comparison of the models obtained with PyVaporation with models provided in the literature indicated similar accuracy of the obtained results, thereby proving the applicability of the developed package.
Powder Injection Molding of Ti-Al-W Nano/Micro Bimodal Powders: Structure, Phase Composition and Oxidation Kinetics
Authors: Krinitcyn Maksim , Pervikov Alexander , Kochuev Dmitriy , Lerner Marat
Published: August 2022

Products from the materials of the Ti-Al system are difficult to manufacture. This often restricts the use of such materials despite their outstanding properties. Some of the promising methods for manufacturing products, which allows working with materials of the Ti-Al system, are powder injection molding (PIM) and material extrusion additive manufacturing (MEAM) technologies. In the present study, powder composites Ti-48Al-4W with different powder size distribution, obtained by the electric explosion of wire (EEW) method, were investigated. The powder was used in PIM technology to produce bulk samples. After polymer debinding, PIM samples were sintered in a vacuum and using hot isostatic pressing (HIP) at the same temperatures and isothermal holding times. The results show the influence of size distribution and sintering method on the structure, phase composition, mechanical properties and oxidation resistance of pre-sintered PIM samples. It is found that both the size distribution and sintering method affect the mechanical properties. The smaller the particle size of the powder in the material, the greater the resistance to oxidation of such samples.
Design and Prototype Fabrication of a Cost-Effective Microneedle Drug Delivery Apparatus Using Fused Filament Fabrication, Liquid Crystal Display and Semi-Solid Extrusion 3D Printing Technologies
Authors: Papadimitriou Petros , Andriotis Eleftherios G. , Fatouros Dimitrios , Tzetzis Dimitrios
Published: August 2022

The current study describes the design of a cost-effective drug delivery apparatus that can be manufactured, assembled, and utilized as easily and quickly as possible, minimizing the time and expense of the supply chain. This apparatus could become a realistic alternative method of providing a vaccine or drug in harsh circumstances, including humanitarian disasters or a lack of medical and nursing staff, conditions that are frequently observed in developing countries. Simultaneously, with the use of microneedles (MNs), the apparatus can benefit from the numerous advantages offered by them during administration. The hollow microneedles in particular are internally perforated and are capable of delivering the active substance to the skin. The apparatus was designed with appropriate details in computer aided design software, and various 3D printing technologies were utilized in order to fabricate the prototype. The parts that required minimum accuracy, such as the main body of the apparatus, were fabricated with fused filament fabrication. The internal parts and the hollow microneedles were fabricated with liquid crystal display, and the substance for the drug loading carrier, which was an alginate gel cylinder, was fabricated with semi-solid extrusion 3D printing.
Babesia, Theileria, Plasmodium and Hemoglobin
Authors: Sojka Daniel , Jalovecká Marie , Perner Jan
Published: August 2022

The Propagation of Plasmodium spp. and Babesia/Theileria spp. vertebrate blood stages relies on the mediated acquisition of nutrients available within the host’s red blood cell (RBC). The cellular processes of uptake, trafficking and metabolic processing of host RBC proteins are thus crucial for the intraerythrocytic development of these parasites. In contrast to malarial Plasmodia, the molecular mechanisms of uptake and processing of the major RBC cytoplasmic protein hemoglobin remain widely unexplored in intraerythrocytic Babesia/Theileria species. In the paper, we thus provide an updated comparison of the intraerythrocytic stage feeding mechanisms of these two distantly related groups of parasitic Apicomplexa. As the associated metabolic pathways including proteolytic degradation and networks facilitating heme homeostasis represent attractive targets for diverse antimalarials, and alterations in these pathways underpin several mechanisms of malaria drug resistance, our ambition is to highlight some fundamental differences resulting in different implications for parasite management with the potential for novel interventions against Babesia/Theileria infections.
Preiswerkite: A First Occurrence in Marble Hosting Gem Spinel Deposits, Luc Yen, Vietnam
Authors: Krivovichev Vladimir G. , Kuksa Katherine A. , Sokolov Pavel B. , Marakhovskaya Olga Yu. , Zolotarev Andrey A. , Bocharov Vladimir N. , Semenova Tatyana F. , Klimacheva Maria E. , Gussiås Geir Atle
Published: August 2022

We report a new occurrence of preiswerkite, the rare sodium analog of eastonite, the trioctahedral mica, from marble-hosted noble spinel deposits of the Luc Yen district, northern Vietnam. It is found in marble for the first time. The preiswerkite is anhedral and associated with phlogopite, aspidolite, sadanagaite, pargasite, spinel, corundum, dolomite and calcite. The average compositions of preiswerkite is (Na0.88Ca0.08K0.01)Σ0.97(Mg2.29Al0.72Fe0.04)Σ3.05)[(Al1.95Si2.05)Σ4.00O10](OH)2. The compositions of preiswerkite have a narrow range of Mg# values (0.96–0.99) and define a preiswerkite-aspidolite solid-solution series. Compared with other occurrences, the Luc Yen preiswerkite has a low iron content, which attains 0.09 atoms per formula unit (1.53 wt.% FeO). The formation of preiswerkite is favored by the proportion of Mg, Al and Si in the precursor rocks and the increased activity of sodium and H2O in the fluid phase.
Investigation of the Deformation Failure Occurring When Extracting Minerals via Underground Mining: A Case Study
Authors: Liu Xuanting , Chen Congxin , Liu Xiumin , Xia Kaizong , Wang Tianlong
Published: August 2022

Metal mines mined using the sublevel caving method often exhibit various environmental problems on the ground surface. This can affect the safety of the production process in the mining area. A numerical model using Universal Distinct Element Code has been established to investigate deformation failure giving rise to this underground mining. The calculations are combined with in-situ monitoring data collected over a period of 10 years. The results indicate that the strata movement in the footwall can be divided into two stages: an arch caving development stage and post arch caving development stage. Mining disturbance is the main cause of caving in the arch caving development stage. The overlying strata experience ‘caving–stability–caving’, leading to the formation of an ‘arch-shaped’ caving pattern. In the post arch caving development stage, flexural-toppling deformation occurs in the strata in the direction of the collapse pit due to the tectonic stress present and high dip angles of the discontinuities. Through-going failure surface has been analyzed by studying the plastic state and displacement of elements. The formation of through-going failure surface is related to the flexural-toppling deformation and stress concentration caused by mining activities. Based on the different failure mechanisms, an efficient partition has been proposed such that the footwall can be divided into a stable zone, flexural-toppling failure zone, compression and slipping-toppling failure zone, and shear-slipping failure zone. The results are a useful reference when applied to the Chengchao Iron Mine and other similar metal mines.
Targeting HIF-1α by Natural and Synthetic Compounds: A Promising Approach for Anti-Cancer Therapeutics Development
Authors: Ghosh Rituparna , Samanta Priya , Sarkar Rupali , Biswas Souradeep , Saha Prosenjit , Hajra Subhadip , Bhowmik Arijit
Published: August 2022

Advancement in novel target detection using improved molecular cancer biology has opened up new avenues for promising anti-cancer drug development. In the past two decades, the mechanism of tumor hypoxia has become more understandable with the discovery of hypoxia-inducible factor-1α (HIF-1α). It is a major transcriptional regulator that coordinates the activity of various transcription factors and their downstream molecules involved in tumorigenesis. HIF-1α not only plays a crucial role in the adaptation of tumor cells to hypoxia but also regulates different biological processes, including cell proliferation, survival, cellular metabolism, angiogenesis, metastasis, cancer stem cell maintenance, and propagation. Therefore, HIF-1α overexpression is strongly associated with poor prognosis in patients with different solid cancers. Hence, pharmacological targeting of HIF-1α has been considered to be a novel cancer therapeutic strategy in recent years. In this review, we provide brief descriptions of natural and synthetic compounds as HIF-1α inhibitors that have the potential to accelerate anticancer drug discovery. This review also introduces the mode of action of these compounds for a better understanding of the chemical leads, which could be useful as cancer therapeutics in the future.
Thermomagnetic-Responsive Self-Folding Microgrippers for Improving Minimally Invasive Surgical Techniques and Biopsies
Authors: Dunn Caleigh R. , Lee Bruce P. , Rajachar Rupak M.
Published: August 2022

Traditional open surgery complications are typically due to trauma caused by accessing the procedural site rather than the procedure itself. Minimally invasive surgery allows for fewer complications as microdevices operate through small incisions or natural orifices. However, current minimally invasive tools typically have restricted maneuverability, accessibility, and positional control of microdevices. Thermomagnetic-responsive microgrippers are microscopic multi-fingered devices that respond to temperature changes due to the presence of thermal-responsive polymers. Polymeric devices, made of poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAM-AAc) and polypropylene fumarate (PPF), self-fold due to swelling and contracting of the hydrogel layer. In comparison, soft metallic devices feature a pre-stressed metal bilayer and polymer hinges that soften with increased temperature. Both types of microdevices can self-actuate when exposed to the elevated temperature of a cancerous tumor region, allowing for direct targeting for biopsies. Microgrippers can also be doped to become magnetically responsive, allowing for direction without tethers and the retrieval of microdevices containing excised tissue. The smaller size of stimuli-responsive microgrippers allows for their movement through hard-to-reach areas within the body and the successful extraction of intact cells, RNA and DNA. This review discusses the mechanisms of thermal- and magnetic-responsive microdevices and recent advances in microgripper technology to improve minimally invasive surgical techniques.
Triflamides and Triflimides: Synthesis and Applications
Authors: Moskalik Mikhail Y. , Astakhova Vera V.
Published: August 2022

Among the variety of sulfonamides, triflamides (CF3SO2NHR, TfNHR) occupy a special position in organic chemistry. Triflamides are widely used as reagents, efficient catalysts or additives in numerous reactions. The reasons for the widespread use of these compounds are their high NH-acidity, lipophilicity, catalytic activity and specific chemical properties. Their strong electron-withdrawing properties and low nucleophilicity, combined with their high NH-acidity, makes it possible to use triflamides in a vast variety of organic reactions. This review is devoted to the synthesis and use of N-trifluoromethanesulfonyl derivatives in organic chemistry, medicine, biochemistry, catalysis and agriculture. Part of the work is a review of areas and examples of the use of bis(trifluoromethanesulfonyl)imide (triflimide, (CF3SO2)2NH, Tf2NH). Being one of the strongest NH-acids, triflimide, and especially its salts, are widely used as catalysts in cycloaddition reactions, Friedel–Crafts reactions, condensation reactions, heterocyclization and many others. Triflamides act as a source of nitrogen in C-amination (sulfonamidation) reactions, the products of which are useful building blocks in organic synthesis, catalysts and ligands in metal complex catalysis, and have found applications in medicine. The addition reactions of triflamide in the presence of oxidizing agents to alkenes and dienes are considered separately.
Enhanced Room Temperature Ammonia Gas Sensing Properties of Fe-Doped MoO3 Thin Films Fabricated Using Nebulizer Spray Pyrolysis
Authors: Alkallas Fatemah H. , Ben Gouider Trabelsi Amira , Shkir Mohd , AlFaify Salem
Published: August 2022

MoO3 thin films are fabricated using nebulizer spray pyrolysis technique, which is doped with Fe at various concentrations of 1, 2, 3, and 4% for ammonia gas sensors application at room temperature. X-ray diffraction (XRD) study confirms the growth of the crystal by Fe doping up to 3%, nano rods shape morphology of the thin film samples observed by field emission scanning electron microscope (FESEM), reduction in bandgap is evidenced via UV-VIS spectrophotometer. Gas sensing study is performed using gas analyzing chamber attached with Keithley source meter. Since 3% Fe doped MoO3 sample displayed nano rods over the film surface which exhibits highest sensitivity of 38,500%, in a short period of raise and decay time 54 and 6 s. Our findings confirms that the 3% Fe doped MoO3 films suitability for ammonia gas sensing application.
Green, Sustainable Synthesis of γ-Fe2O3/MWCNT/Ag Nano-Composites Using the Viscum album Leaf Extract and Waste Car Tire for Removal of Sulfamethazine and Bacteria from Wastewater Streams
Authors: Khalatbary Mansooreh , Sayadi Mohammad Hossein , Hajiani Mahmood , Nowrouzi Mohsen , Homaeigohar Shahin
Published: August 2022

Multi-walled carbon nanotubes (MWCNTs) decorated with Ag nanoparticles (NPs) are bifunctional adsorbent nanomaterials with antibacterial activity. They can be magnetically recovered from wastewater in case of coupling with γ-Fe2O3. In this study, for the first time, an environmentally friendly technique was applied to prepare a nanocomposite (NC) material composed of γ-Fe2O3/MWCNT/Ag by using Bridgestone disposable tires and Viscum album leaves extract. γ-Fe2O3/MWCNTs/Ag NC was employed for the removal of sulfamethazine (SMT) from aqueous solutions. Under the optimized conditions determined via the Taguchi method, the highest SMT adsorption capacity of the γ-Fe2O3/MWCNT/Ag NC was measured to be 47.6 mg/g. The experimental data fitted well with the pseudo-second-order kinetic model and the Langmuir isotherm. The thermodynamic parameters implied that the adsorption process was endothermic. In addition to adsorption of the drug pollutant, the NC demonstrated a superior antibacterial activity against Gram-positive bacteria. The reusability test also showed that over 79% SMT can be removed using γ-Fe2O3/MWCNTs/Ag NC even after four adsorption cycles. Taken together, γ-Fe2O3/MWCNTs/Ag NC was proven to be a promising antibacterial nano-adsorbent for wastewater treatment.
Particle Distribution and Heat Transfer of SiO2/Water Nanofluid in the Turbulent Tube Flow
Authors: Shi Ruifang , Lin Jianzhong , Yang Hailin
Published: August 2022

In order to clarify the effect of particle coagulation on the heat transfer properties, the governing equations of nanofluid together with the equation for nanoparticles in the SiO2/water nanofluid flowing through a turbulent tube are solved numerically in the range of Reynolds number 3000 ≤ Re ≤ 16,000 and particle volume fraction 0.005 ≤ φ ≤ 0.04. Some results are validated by comparing with the experimental results. The effect of particle convection, diffusion, and coagulation on the pressure drop ∆P, particle distribution, and heat transfer of nanofluid are analyzed. The main innovation is that it gives the effect of particle coagulation on the pressure drop, particle distribution, and heat transfer. The results showed that ∆P increases with the increase in Re and φ. When inlet velocity is small, the increase in ∆P caused by adding particles is relatively large, and ∆P increases most obviously compared with the case of pure water when the inlet velocity is 0.589 m/s and φ is 0.004. Particle number concentration M0 decreases along the flow direction, and M0 near the wall is decreased to the original 2% and decreased by about 90% in the central area. M0 increases with increasing Re but with decreasing φ, and basically presents a uniform distribution in the core area of the tube. The geometric mean diameter of particle GMD increases with increasing φ, but with decreasing Re. GMD is the minimum in the inlet area, and gradually increases along the flow direction. The geometric standard deviation of particle diameter GSD increases sharply at the inlet and decreases in the inlet area, remains almost unchanged in the whole tube, and finally decreases rapidly again at the outlet. The effects of Re and φ on the variation in GSD along the flow direction are insignificant. The values of convective heat transfer coefficient h and Nusselt number Nu are larger for nanofluids than that for pure water. h and Nu increase with the increase in Re and φ. Interestingly, the variation in φ from 0.005 to 0.04 has little effect on h and Nu.
Gram-Scale Synthesis of Graphitic Carbon Nitride Quantum Dots with Ultraviolet Photoluminescence for Fe3+ Ion Detection
Authors: Lu Xuemei , Qin Haijun , Cai Jiuzhang , Cui Yuhang , Liao Lixin , Lv Fengzhen , Zhu Changming , Wang Liguang , Liu Jun , Long Lizhen , Kong Wenjie , Liu Fuchi
Published: August 2022

A method for gram-scale synthesis of graphitic carbon nitride quantum dots (g-C3N4QDs) was developed. The weight of the g-C3N4QDs was up to 1.32 g in each run with a yield of 66 wt%, and the purity was 99.96 wt%. The results showed that g-C3N4QDs exhibit a stable and strong ultraviolet photoluminescence at a wavelength of 365 nm. More interestingly, the g-C3N4QDs can be used as a high-efficiency, sensitive, and selective fluorescent probe to detect Fe3+ with a detection limit of 0.259 μM.
Factors Associated with Low Vitamin D Status among Older Adults in Kuwait
Authors: ALbuloshi Thurayya , Kamel Ahmed M. , Spencer Jeremy P. E.
Published: August 2022

Low vitamin D levels among older people represent a significant health problem worldwide. This study aimed to examine the factors associated with vitamin D deficiency in older people (aged ≥ 65) in the Kuwaiti population. A cross-sectional study was conducted in seven primary healthcare centers across Kuwait (November 2020 to June 2021). The participants (n = 237) had their serum vitamin D (25(OH)D) concentrations (analyzed using LC-MS) classified as sufficiency 75 nmol/L (30 ng/mL) or deficiency < 75 nmol/L (below 30 ng/mL). The data were collected using self-administered questionnaires and face-to-face interviews with participants in geriatric clinics. Binomial logistic regression analysis was applied to assess factors associated with vitamin D deficiency. Vitamin D deficiency was found to be present in two thirds of the participants (n = 150, 63%), with a higher prevalence of deficiency in participants who did not receive vitamin D supplements, compared to those who did (84% vs. 16%, p = 0.001). The results from the binary logistic regression showed that a low duration of sun exposure (OR = 0.24, 95% C.I. [0.08–0.7], p = 0.011), dark skin pigmentation (OR = 4.46, 95% [1.35–20.49], p = 0.026), and lower caloric intake (OR = 0.9, 95% C.I. [0.85–0.96], p = 0.001) were risk factors for vitamin D deficiency. Furthermore, a significant inverse relationship was found between vitamin D levels and parathyroid hormone (PTH) levels (OR = 1.16, 95% C.I. [1.04–1.31], p = 0.016). These findings support the recommendation that vitamin D supplementation and adequate sunlight exposure are necessary for raising low vitamin D levels in older people in Kuwait.
Prevalence and Correlates of Metabolic Syndrome and Its Components in Chinese Children and Adolescents Aged 7–17: The China National Nutrition and Health Survey of Children and Lactating Mothers from 2016–2017
Authors: Shi Jia , He Li , Yu Dongmei , Ju Lahong , Guo Qiya , Piao Wei , Xu Xiaoli , Zhao Liyun , Yuan Xiaolin , Cao Qiuye , Fang Hongyun
Published: August 2022

This descriptive study aimed to determine the prevalence of metabolic syndrome (MetS) and its components among Chinese children and adolescents aged 7–17 from 2016–2017 according to the Cook’s criteria modified for age on the basis of the National Cholesterol Education Program-Adult Treatment Panel III (NCEP-ATP III) and to evaluate the associations between the factors of interest (especially vitamin A, vitamin D and hyperuricemia) of MetS and its components, using data from the China National Nutrition and Health Survey of Children and Lactating Mothers from 2016–2017. A total of 54,269 school-aged children and adolescents were ultimately included in this study. Anthropometric measurements and laboratory examinations of the subjects and their relevant information were also collected. A multivariate logistic regression analysis model was applied to analyze the relationships between relevant factors associated with MetS and its components. In the present study, the prevalence of MetS in children and adolescents was 5.98%. Among the five components of MetS, elevated blood pressure (BP) and abdominal obesity were the most prevalent (39.52% and 17.30%), and 58.36% of the subjects had at least one of these components. In the multivariate logistic regression, an overweight condition, obesity and hyperuricemia were positively correlated with the incidence of MetS and all five components. There was also a positive association observed between vitamin A and the risk of MetS and some components of MetS (abdominal obesity and high triglycerides (TG)) and vitamin A was negatively associated with the risk of low high-density lipoprotein cholesterol (HDL-C). Subjects with vitamin D inadequacy had a higher risk of MetS (OR = 1.364, 95%CI: 1.240–1.500) and four of its components, excepting elevated FBG (fast blood glucose). Vitamin D deficiency was positively associated with MetS (OR = 1.646, 95%CI: 1.468–1.845) and all five of its components. Well-designed, large-scale prospective studies are also needed in the future.
Effects of Spermidine on Gut Microbiota Modulation in Experimental Abdominal Aortic Aneurysm Mice
Authors: Liu Shuai , Liu Yu , Zhao Jiani , Yang Pu , Wang Wei , Liao Mingmei
Published: August 2022

Accumulating evidence in recent years has demonstrated the important role of gut microbiota in maintaining cardiovascular function. However, their functions in abdominal aortic aneurysm (AAA) are largely unexplored. In this study, we established a porcine pancreatic elastase-infused experimental AAA mouse model and explored gut microbiota modulation using 16S rDNA sequencing. Here, we found that a significant alteration to gut microbiota composition and function occurred in AAA. The functional change in the gut microbiome revealed dysregulated biosynthesis metabolism and transport of spermidine in AAA. Furthermore, exogenous spermidine was administrated via drinking water and attenuated the progression of experimental AAA disease, which supports our recent study that spermidine alleviates systemic inflammation and AAA. These effects were associated with remitted gut microbiota dysbiosis and metabolism in AAA progression as demonstrated by 16S rDNA gene analysis. In addition, several bacterial florae, such as Bacteroides, Parabacteroides and Prevotella, were identified to be associated with the progression of AAA. Our results uncovered altered gut microbial profiles in AAA and highlighted the potential therapeutic use of spermidine in the treatment of gut microbiota dysbiosis and AAA.
Recombinant Decoy Exhibits Broad Protection against Omicron and Resistance Potential to Future Variants
Authors: Tang Haoneng , Ke Yong , Wang Lei , Wu Mingyuan , Sun Tao , Zhu Jianwei
Published: August 2022

The Omicron variant has swept through most countries and become a dominant circulating strain, replacing the Delta variant. The evolutionary history of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) suggests that the onset of another variant (possibly another variant of concern (VOC) is inevitable. Therefore, the development of therapeutics that enable treatments for all Omicron-included VOCs/variants of interest (VOIs) and future variants is desired. Recently, the recombinant receptor decoy therapeutic angiotensin-converting enzyme 2 (ACE2)-Fc has exhibited good safety in a phase 1 clinical trial; therefore, its variant-resistant profile needs to be understood. Here, we conducted a comprehensive evaluation of its neutralization breadth against the Omicron variant and other VOCs/VOIs. Furthermore, to evaluate its resistance to future variants, we investigated its ability to neutralize various single-residue mutated variants. Next, we demonstrated its resistance to evasion via an experiment that rapidly and effectively stimulates virus evolution with a replication-competent virus model. In addition, we evaluated its efficacy for cocktail therapy. The combination of ACE2-Fc and neutralizing antibodies showed both efficacy and breadth in the simulation experiment. The underlying mechanism was revealed to be a synergistic effect in the cocktails. Collectively, this study deepens the understanding of the resistance profile of recombinant receptor decoy therapeutics and highlights the potential value of ACE2-Fc and neutralizing antibody cocktails in the subsequent anti-SARS-CoV-2 campaign. Furthermore, we also provide an effective method to study the resistance profile of antiviral agents and rapidly screen for potential cocktails to combat future variants.