bluuebunny/crossref_metadata_2025 huggingface dataset:
How to choose kinematic or mechanical alignment individually according to preoperative characteristics of patients?
Authors: Luan Chong , Xu Dong-Tan , Chen Ning-Jie , Wang Fei-Fei , Tian Kang- Song , Wei Chao , Wang Xian-Bin
Published: July 2020

Background Making decisions in alignment techniques in total knee arthroplasty (TKA) remains controversial. This study aims to identify the potential patients who were suitable for the kinematic (KA) or mechanical alignment (MA). Methods We reviewed 296 consecutive patients (296 TKAs, including 114 KA-TKAs and 182 MA-TKAs) who underwent unilateral TKA using a computer-assisted navigation from 2016 to 2018 in our prospectively maintained database. The minimum followup was one year. Clinical outcomes including the range of motion (ROM) and knee society score (KSS) were compared between KA-TKAs and MA-TKAs. Multiple regression models were used to evaluate the relationship between alignment techniques and KSS at the 1-year followup. Interaction and stratified analyses were conducted according to gender, age, body mass index (BMI), preoperative hip-knee-ankle (HKA) angle, ROM and KSS.Results ROM and KSS at the 1-year followup didn't differ between MA-TKAs and KA-TKAs (all p>0.05). Alignment techniques did not associate with postoperative ROM (Adjusted β=0.4, 95% confidence interval [CI]: -0.3, 1.6; p=0.752) or 1-year KSS (Adjusted β=2.2, 95%CI: -0.7, 5.6; p=0.107). Patients with a BMI more than 30 kg/m^2 achieved better 1-year KSS when using MA than KA (p for interaction<0.05). Additionally, patients with preoperative HKA angle more than 10 degrees varus benefited more from KA than MA (p for interaction<0.05).Conclusions Patients with severe varus deformity may be suitable for the KA technique, whereas MA should be used in obese patients.
Overexpression of AGAMOUS-like gene PfAG5 promotes early flowering in Polypogon fugax
Authors: Zhou Fengyan , Yu Qin , Zhang Yong , Yao Chuan-Chun , Han Yun-Jing , Shen Yan
Published: July 2020

Background: Herbicides are the major tool for controlling large populations of yield depleting weeds. However, overreliance on herbicides has resulted in weed adaptation and herbicide resistance. In recent years, early flowering weed species related to herbicide resistance is emerging, which may cause seed loss before crop harvest, creating a new problem for non-chemical weed management. However, mechanisms regulating early flowering in weedy species is rarely investigated. Results: The MADS-box gene family plays an important role in flowering time regulation and floral organogenesis. In this study, a homolog gene of AGAMOUS sub-family (referred to as PfAG5) of the MADS-box family was cloned from plants of an early flowering Polypogon fugax population resistant to the ACCase inhibitor herbicide (clodinafop-propargyl). The PfAG5 gene was functionally characterized in Arabidopsis thaliana. Over-expression of the PfAG5 gene in Arabidopsis resulted in early flowering with abnormal flowers (e.g. small petals, short plants and reduced seed set) compared to the wild type. The expression of the PfAG5 gene was high in leaves and flowers, but low in pods in transgenic Arabidopsis. The PfAG5 gene was earlier and higher expressed in the resistant (R) than the susceptible (S) P. fugax plants. Furthermore, one protein (FRIGIDA-like protein) interacting with PfAG5 in R P. fugax was identified by the yeast two-hybrid system with relevance to flowering time regulation. Conclusions: These results suggest that the PfAG5 gene is involved in modulating early flowering in P. fugax. This study provides the first evidence for the regulation mechanism of early flowering in an herbicide resistant weed species.
Beliefs, perceptions and practices of chiropractors and patients about mitigation strategies for benign adverse events after spinal manipulation therapy
Authors: Funabashi Martha , Pohlman Katherine A. , Goldsworthy Rachel , Lee Alex , Tibbles Anthony , Mior Silvano , Kawchuk Greg
Published: July 2020

Background: Approximately 50% of patients who receive spinal manipulative therapy (SMT) experience some kind of adverse event (AE), typically benign and transient in nature. Regardless of their severity, mitigating benign AEs is important to improve patient experience and quality of care. The aim of this study was to identify beliefs, perceptions and practices of chiropractors and patients regarding benign AEs post-SMT and potential strategies to mitigate them.Methods: Clinicians and patients from two chiropractic teaching clinics were invited to respond to an 11-question survey exploring their beliefs, perceptions and practices regarding benign AEs post-SMT and strategies to mitigate them. Responses were analyzed using descriptive statistics.Results: A total of 39 clinicians (67% response rate) and 203 patients (82.9% response rate) completed the survey. Most clinicians (97%) believed benign AEs occur, and 82% reported their own patients have experienced one. For patients, 55% reported experiencing benign AEs post-SMT, with the most common symptoms being pain/soreness, headache and stiffness. While most clinicians (61.5%) reported trying a mitigation strategy with their patients, only 21.2% of patients perceived their clinicians had tried any mitigation strategy. Clinicians perceived that patient education is most likely to mitigate benign AEs, followed by soft tissue therapy and/or icing after SMT. Patients perceived stretching was most likely to mitigate benign AEs, followed by education and/or massage.Conclusions: This is the first study comparing beliefs, perceptions and practices from clinicians and patients regarding benign AEs post-SMT and strategies to mitigate them. This study provides an important step towards identifying the best strategies to improve patient safety and improve quality of care.
Process evaluation of an evidence-based weight loss program for low-income, mid-life women implemented in local health departments: Application of the RE-AIM framework
Authors: Norwood Arnita F. , Linnan Laura , Ammerman Alice S. , Cai Jianwen , Ward Dianne S. , Samuel-Hodge Carmen D.
Published: July 2020

Background: Fundamental to successfully translating evidence-based interventions to real life settings with diverse populations is achieving fidelity to the intervention within the given public health setting. Health departments may be key in addressing the obesity epidemic given their unique position to deliver obesity prevention services in community settings. We developed the Weight-Wise II Program from four evidenced-based interventions for implementation in local health departments. For this study, we conducted a process evaluation of the implementation of the Weight-Wise II Program, an intensive evidence-based behavioral weight loss program for low-income, mid-life women. Methods: The Weight-Wise II Program, a 16-week group-based weight loss program, was implemented in six local health departments. The RE-AIM framework (reach, effectiveness, adoption, implementation, and maintenance) served as a guide to conduct a comprehensive process evaluation. Process data were collected and analyzed using quantitative and qualitative methods. Results: The Weight-Wise II Program reached low-income and mid-life women, a high-risk population, and was effective in achieving modest weight loss. Attendance and self-monitoring were positively associated with weight loss. Interventionists delivered the program as intended and it was well received by participants. Planned adaptations enhanced program implementation by being responsive to participant and health department needs. Despite implementation by health department staff, the program was not routinely maintained three years post-intervention. Conclusion: Evidence-based weight loss interventions can be successfully implemented in local health departments. RE-AIM is a useful framework for systematically evaluating the process of implementation and impact of a behavioral weight loss intervention offered in public health settings. The addition of complementary implementation frameworks may help in identification of contextual factors influencing subsequent maintenance of evidence-based interventions in public health settings. Trial registration: ClinicalTrials.gov Identifier: NCT01141348. Registered 9 June 2010 – Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT01141348
Improvement of health-related quality of life and radiographic parameters in adolescent idiopathic scoliosis patients after Schroth exercises
Authors: Gao Ang , Li Junyu , Shao Rui , Yu Miao , Wu Tongxuan , Wang Yongqiang , Liu Xiaoguang
Published: July 2020

Background Finding an optimal treatment strategy for adolescent idiopathic scoliosis (AIS) patients remains challenging because of its intrinsic complexity. For mild to moderate scoliosis patients with lower skeletal growth potential (Risser 3–5), most clinicians agree with observation treatment; however, the curve progression that occurs during puberty, the adolescent period, and even in adulthood, remains a challenging issue for clinicians. The aim of study is to investigate the efficacy of Schroth exercise in adolescent idiopathic scoliosis (AIS) patients with lower skeletal growth potential (Risser 3-5) and moderate scoliosis (Cobb angle 20-40°). Methods Data from 43 patients diagnosed with AIS and performed Schroth exercise were reviewed, and outcomes were measured by health-related quality of life (HRQOL) and radiographic parameters. HRQOL was assessed using the visual analog scale (VAS) scores for back, Scoliosis Research Society-22 (SRS-22) patient questionnaire. Radiographic spinopelvic parameters were obtained from anteroposterior and lateral X-rays. The pre-treatment and post-treatment HRQOL and radiographic parameters were tested to validate Schroth exercise efficacy.Results Vas back score, SRS-22 pain and SRS-22 self-image domain domain were significantly improved from pre-treatment 3.0±0.8, 3.6±0.5 and 3.5±0.7 to post-treatment 1.6±0.6, 4.0±0.3 and 3.7±0.4, respectively. No significant improvement of SRS-22 function domain and mental health domain. The mean Cobb angle decreased from 28.9±5.5° to 26.3±5.2° at the final follow-up, despite no statistical significance was observed. The mean C2-C7 sagittal vertical axis (SVA) value decreased from 21.7±8.4 mm to 17.0±8.0mm and mean T1 tilt decreased from 4.9±4.2 ° to 3.5±3.1°. No other significant improvement of radiographic parameters was observed.Conclusions For AIS patients with a Risser 3–5 and a Cobb angle 20°–40°, Schroth exercises improved HRQOL and halted curve progression during the follow-up period. Both cervical spine alignment and shoulder balance were also significantly improved after Schroth exercises. We recommend Schroth exercises for patients with AIS.
Estimating The Under-Ascertainment Number of COVID-19 Cases in Kano, Nigeria in The Fourth Week of April 2020: A Modelling Analysis of The Early Outbreak
Authors: Musa Salihu S , Zhao Shi , Hussaini Nafiu , Zuang Zian , Wang Maggie H , He Daihai
Published: July 2020

Background: The coronavirus disease 2019 (known as COVID-19) pandemic caused by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) appeared in Wuhan, China has rapidly spread to over 200 countries and territories. In Nigeria, the Kano State Ministry of Health has confirmed its first case of COVID-19 on April 11, 2020, and since then there might have been issues of under-ascertainment that occurred roughly from 22 to 27 April 2020. In this work, we estimate the number of under-ascertainment cases and the basic reproduction number, B, of COVID-19 in Kano, Nigeria. Methods: We employ the exponential growth and modelled the outbreak curve of COVID-19 cases, in Kano, Nigeria from 11 to 30 April 2020. We estimated the number of under-ascertainment cases using the maximum likelihood estimation. We adopted the SI estimated for Hong Kong as approximations of the unknown SI for COVID-19 in Kano to estimate the a. We use ARIMA model to provide a short term (15 days) prediction of the COVID-19 cases in Kano, Nigeria.Results: We revealed that the initial growth phase mimic an exponential growth pattern. We found that the under-ascertainment was likely to have resulted in 213 (95% CI: 106−346) unreported cases from 22 to 27 April 2020. The reporting rate after 27 April 2020 increase up to 10-fold compared to the scenario from 22 to 27 April 2020 on average. We estimated the c of COVID-19 in Kano as 2.74 (95% CI: 2.53−2.96). We forecasted that the total number of COVID-19 cases in Kano to be 1067 (95% CI: 883, 2137) by June 6, 2020.Conclusion: The under-ascertainment likely exists during the fourth week of April, 2020 and should be regarded in the future analysis/investigation.
Randomized controlled trial of exercise interventions for chronic whiplash-associated disorders: One-year follow-up of the effect on neck muscle endurance, active cervical range of motion and grip strength.
Authors: Peterson Gunnel , Ludvigsson Maria Landén , Peolsson Anneli
Published: July 2020

Background: Years after whiplash injury, more than 40 % of people experience persistent neck pain and disability, called whiplash-associated disorders (WAD). The relations between neck related function, neck pain and disability are inconclusive and need to be further investigated. The aims were; a) to compare the effect of three different exercise interventions – neck-specific exercise (NSE), NSE with a behavioral approach (NSEB) and prescribed physical activity (PPA) – on neck-muscle endurance (NME), active cervical range of motion (AROM), grip strength and pain intensity immediately before and after the tests, and b) to compare neck-disability in individuals who are below or above the cut-off for normative reference values of healthy individuals regarding NME, AROM and grip strength.Methods: This is a secondary analysis of a randomized controlled multicenter study including 12 months’ follow-up. A total of 216 individuals with chronic WAD grades II or III were recruited. Data were recorded at baseline, 3, 6, and 12 months. Linear mixed models were used and sub-group analyses were evaluated with non-parametric tests.Results: NSE and NSEB resulted in greater improvements compared to PPA (p < .01) and were maintained at 12 months’ follow-up in ventral (only males) and dorsal NME, AROM and pain intensity during testing. In grip strength, there were no significant between-group differences (p > 0.05). There were no significant differences between the NSE and NSEB groups (p > .05). Sub-group analyses revealed improvement in disability at 12 months follow-up in NSE and/or NSEB for individuals both below and above the cut-off reference value in NME and AROM. More severe disability was seen in individuals below the reference values both at baseline and 12 months’ follow-up. Individuals in the PPA group below the reference values in NME and AROM reported increasing disability at 12 months compared to baseline.Conclusion: The results suggest that neck-specific exercises (NSE, NSEB) improved clinical function and decreased disability in chronic WAD compared to PPA. The results may imply that higher neck-related clinical function can be important for reduced disability. Trial Registration: ClinicalTrials.gov: NCT01528579, date of registration: February 8, 2012, retrospectively registered; https://clinicaltrials.gov/ct2/show/NCT0152857
Long-Acting GnRH Agonist Improves IVF Outcomes of Young Patients with Diminished Ovarian Reserve by Increasing Endometrial Receptivity.
Authors: Liu xuemei , Bao Hongchu , Qu Qinglan , Guo Zhenyu , Li Wenshu , Zhao Huishan
Published: July 2020

Background: Up to now, there is not sufficient evidence to recommend a treatment optimizing in vitro fertilization (IVF) outcomes for diminished ovarian reserve (DOR). This study is aimed to investigate whether long-acting gonadotrophin-releasing hormone (GnRH) agonist long protocol in follicular phase could improve the IVF cycle outcomes for young patients with DOR when compared with GnRH antagonist and mild ovarian stimulation protocols. Methods: This retrospective cohort study was carried out from June 2015 to March 2019. 338 patients aged 20-40 years with DOR who underwent first IVF between were enrolled. These patients were assigned to three groups depending on the ovarian stimulation protocols. The outcome parameters of IVF were compared in each group. The demographic and reproductive characteristics were calculated by Mann-Whitney, Kruskal-Wallis or chi-square test as appropriate. We evaluated the clinical outcomes of IVF cycle between the three groups using univariate and multivariate logistic regression analyses. In addition, we evaluated the morphology and coverage of pinopode and expression of HOXA10 in endometrium during implantation window between three groups by scanning electron microscope and qRT-PCR.Results: Patients who received long-acting GnRH agonist long protocol had significantly higher clinical pregnancy rates (66.67%, 42.17% and 39.02%, respectively; P=.010 and .005), implantation rates (46.15%, 29.71%, and 28.57%, respectively; P=.041 and .025) and ongoing pregnancy rates (60.00%, 34.94%, and 36.59%, respectively; P=.018 and .004). They also had significantly higher duration of stimulation, total dose of gonadotrophins and endometrial thickness than the other two groups (P＜.001). However, serum luteinizing hormone (LH) and estradiol (E2) level on gonadotrophins initiation day, serum LH level on human chorionic gonadotropin (hCG) day, the embryos transferred cancellation rate and abnormal endometrium rate were significantly lower among women who received long-acting GnRH agonist (P＜.001). In addition, we found that long-acting GnRH agonist could improve the development of pinopode and mRNA of HOXA10 (P＜.05).Conclusions: To our knowledge, this is the first time that the benefit of long-acting GnRH agonist long protocol in follicular phase in young DOR patients has been reported. Though this novel protocol may have further suppressed response, it can increase endometrial receptivity, reduce cycle cancellation rate and improve IVF cycle clinical outcomes for these patients compared with mild stimulation and GnRH antagonist protocols.
The relationship between delivery mode and postpartum depression: a systematic review and meta-analysis
Authors: Chen Yu-Xin , Zhao Pei , Jiesisibieke Zhu Liduzi , Chen Pei-En , Tung Tao-Hsin , Chien Ching-Wen
Published: July 2020

Background

To assess the relationship between delivery mode and postpartum depression and to examine whether cesarean section (CS) has a higher risk of postpartum depression than vaginal delivery (VD).
Methods

We searched the Cochrane Library, PubMed, and EMBASE from inception to 30 April 2019 without language limitations. Then two authors independently selected the studies, assessed the quality of the included studies, and extracted data. Any disagreements were resolved by discussion with a third author. Next, we used the Newcastle-Ottawa Scale and GRADE methods are used to evaluate the quality of the included studies and evidences, respectively. This study had four included cohort studies data and carried out fixed-effect model meta-analysis.
Results

The findings demonstrated a significant difference in the risk of postpartum depression between CS and VD. Compared with the control group, the CS group was associated with a higher prevalence of postpartum depression symptoms (Risk Ratio = 1.29; 95% CI: 1.11–1.51).
Conclusions

The results showed that delivery mode has nonnegligible effects on the risk of postpartum depression. Particularly, we found that CS is associated with a higher risk of postpartum depression compared with VD. Therefore, we ought to encourage pregnant women who are without medical indication of CS to select VD.
Trial registration:

The protocol of this systematic review was registered in PROSPERO under the number CRD42019148154.
Mid- to long-term outcomes of cementless modular, fluted, tapered stem for massive femoral bone loss in revision total hip arthroplasty
Authors: Zheng Kai , Li Ning , Zhang Weicheng , Zhou Jun , Xu Yaozeng , Geng Dechun
Published: July 2020

Background: Revision total hip arthroplasty is frequently accompanied by bone loss. The purpose of this study is to evaluate mid- to long-term results of revision total hip arthroplasty for massive femoral bone loss using cementless modular, fluted, tapered stems.Methods: During the period of 2007 to 2015 at a single hospital, 34 hips (33 patients) underwent primary revision surgery with cementless modular, fluted, tapered stems due to massive bone loss, and patients with prosthetic joint infection (PJI) or tumours were excluded. The hips were revised with the LINK MP (Waldemar Link, Hamburg, Germany) prosthesis. Bone loss was categorized by the Paprosky classification for prosthesis loosening and Vancouver classification for peri-prosthetic fracture. All revision bearing surfaces were ceramic-on-ceramic (CoC). Clinical outcomes, radiographic outcomes and survivorship were evaluated.Results: The mean follow-up was 9.1±2.5 years (range, 5-13 years). The Harris hip score was 43.6±11.5 preoperatively and maintained at 86.5±6.6 at the time of latest follow-up (p<0. 05). The X-ray showed bone ingrowth fixation in 30 hips (88%), fibrous stable fixation in 3 hips (9%) and instability in 1 hip (3%). The average stem subsidence was 3.9±2.2 mm (range, 1 to 10 mm). The survivorship of prostheses with re-revision for any reason was 95% (95% CI, 12.0 to 13.0) at the 10-year follow-up. Three (9%) re-revisions were needed, including 1 for aseptic loosening, 1 for dislocation and 1 for infection.Conclusions: The mid- to long-term results of revision total hip arthroplasty with the cementless modular, fluted, tapered stem (LINK MP stem) are encouraging for massive femoral bone loss.
Medical Students’ Commitment during the SARS-CoV-2 Pandemic: Preparedness, Motivation, and Impact on Students’ Skills
Authors: Müller Lukas , Heymanns Markus , Harder Laura , Winter Julia , Gehring Stephan , Düber Christoph , Mähringer-Kunz Aline , Kloeckner Roman
Published: July 2020

Background: During the SARS-CoV-2 pandemic, many authors have suggested a commitment of medical students to support overworked health care staff. However, whether the students are prepared for such an occupation remains unclear. Therefore, the aim of this study was to evaluate medical students’ preparedness for a commitment in the pandemic and to assess the impact on their skills and attitudes.Methods: In April 2020, the CoronaPreventMainz (CPM) study was initiated to test 3300 employees with direct patient contact at the University Medical Center Mainz. To accomplish the huge logistic effort, medical students were recruited as support staff.Using a web-based questionnaire, the participating students were asked 27 questions covering six different topics.Results: Of the 75 recruited students, 63 (84.0%) participated in this survey. The median age was 24 years, and 66.6% (n = 42) were female. The vast majority agreed that students should be used as voluntary helpers during this crisis (87.3%) and had the feeling of contributing in the fight against the pandemic (90.5%). Most of the students (80.6%) even reported an improvement in their practical skills. Fear of self-infection was low (7.9%), and overextending situations occurred for just 3.2%. However, less than one-fifth (19.4%) of the students felt prepared for the SARS-CoV-2 pandemic by medical school, and two-thirds (67.7%) demanded special preparation. Conclusion: Through their commitment, the medical students felt that they were taking part in the fight against the pandemic. However, only a few felt well-prepared by medical school and the students’ need for special preparation courses is huge. Therefore, single-center initiatives can only be the beginning. Dedicated courses on how to support health care staff in natural disasters should be integrated into the medical curriculum to better prepare medical students for the next crisis.
Analysis the contraception usage and unmet needs of education and reproductive health in Hubei province, China
Authors: meilin peng , zhao kai , Zhang Huiping , Tian kunming , Fang Yiwei , Zhang yuan
Published: July 2020

background: In recent years, contraceptives have developed rapidly, which are divided into modern contraceptives and non-modern contraceptives. The use of contraceptives less unwanted pregnancy and sexually transmitted infections(STIs) including HIV. And cause traditional attitudes towards sex, marriage and family have changed. It leads to a lack of caution about sex and pre-marital sex is more acceptable. Hence, the number of miscarriages caused by unwanted pregnancies has increased. People is going to settle many of sexual and reproductive health matters. Methods: This study, was conducted of about 103 counties in Hubei Province from August 2014 to July 2016, which used frequencies, percentage, mean, chi-square, logistic regression to analysis this data that collected from 17555 respondents. (IBM-SPSS v 25.0)Results: The results in this article describe more men (62.6%) received family planning services education than women (37.4%). And people who did not participate in family planning services education, 17.0% and 21.9% did not know about vasectomy and withdrew as a method of male contraception, respectively. Striking, up to 23.9% and 22.8% of people with or without participating in family planning services education had experienced contraceptive failure (pregnancy for example) in couple. Age, educational, occupational status and the number of living children were strongly associated with contraceptive failure within participating in family planning services education. Only age, place of residence and number of living children were significant associated with contraceptive failure without participating in family planning services education. The figure showed greatly unmet needs of education and reproductive health whether or not to accept family planning services educationConclusion: There is a huge difference in Knowledge and use of contraceptives, as well as unmet educational and reproductive health needs between those who participated in family planning services education and those who did not. That means people who participate in family planning services education got more about sexual and reproductive health education and understand the important of the family planning services. Therefore, it is necessary to provide family planning services for more people and regions to obtain a good understanding of contraceptives, sexual intercourse and unintended pregnancy.
Economic evaluation of a web-based menu planning intervention to improve childcare service adherence with dietary guidelines.
Authors: Reeves Penny , Edmunds Kim , Szewczyk Zoe , Grady Alice , Yoong Sze Lin , Wolfenden Luke , Wyse Rebecca , Finch Megan , Stacey Fiona , Wiggers John , Searles Andrew
Published: July 2020

Background Despite the known benefits of healthy eating in childhood, few Australian childcare services provide food that is consistent with dietary guidelines. The effectiveness of a web-based menu planning intervention to increase childcare service provision of healthy foods, and decrease provision of discretionary foods in long daycare centres in Australia was assessed in a randomised controlled trial. Here we consider the costs, consequences, cost-effectiveness and budget impact of the intervention using data collected within the trial. Methods The prospective trial-based economic evaluation involved 54 childcare services across New South Wales (NSW), Australia. Services were randomised to a 12-month intervention or usual care. The intervention involved access to a web-based menu planning and decision support tool, and online resources. Effectiveness measures included: mean number of food groups, overall menu and individual food group compliance with dietary guidelines, and mean servings of food groups at 12 months. Costs (reported in $AUD, 2017/18) were evaluated from both health sector and societal perspectives. The direct cost to support uptake of the intervention was calculated, as were costs to each childcare centres. The incremental cost of the intervention was calculated as the net difference in the cost to undertake menu planning and review plus the direct cost of the intervention. Incremental cost–effectiveness ratios (ICERs) including uncertainty intervals, were estimated for differences in costs and effects between intervention and control groups. A relative value index was calculated to determine overall value for money. Results Over the 12 months of the trial, we calculated a difference in cost between usual practice and intervention groups of -$482 (95% UI -$859, -$56). While the measured increase in menu and food group compliance within the trial did not reach statistical significance, there were significant improvements in mean servings of fruit and discretionary food, represented in the cost-consequence analysis. The calculated relative value index of 1.1 suggests that the intervention returns acceptable value for money for the outcomes generated. Conclusion Compared to usual practice, web-based programs may offer an efficient and sustainable alternative for childcare centres to improve the provision of healthy foods to children in their care. Trial Registration Australian New Zealand Clinical Trials Registry ACTRN12616000974404
Transcriptome Response Comparison Between Vector and Non-Vector Aphids After Feeding on Virus-Infected Wheat Plants
Authors: Li Dandan , Zhang Chi , Tong Zeqian , Su Dan , Zhang Gaisheng , Zhang Shize , Zhao Huiyan , Hu Zuqing
Published: July 2020

Background: Plant viruses maintain intricate interactions with their vector and non-vector insects and can impact the fitness of insects. However, the details of their molecular and cellular mechanisms have not been studied well. We compared the transcriptome-level responses in vector and non-vector aphids (Schizaphis graminum and Rhopalosiphum padi) after feeding on wheat plants with viral infections (Barley Yellow Dwarf Virus (BYDV)/Wheat dwarf virus (WDV)). We conducted differentially expressed gene (DEG) annotation analyses and observed DEGs related to immune pathway, growth, development, and reproduction. And we conducted cloning and bioinformatic analyses of the key DEG involved in immune.Results: For all differentially expressed gene analyses, the numbers of DEGs related to immune, growth, development, reproduction and cuticle were higher in vector aphids than in non-vector aphids. STAT5B (signal transducer and activator of transcription 5B), which is involved in the JAK-STAT pathway, was upregulated in R. padi exposed to WDV. The cloning and bioinformatic results indicated that the RpSTAT5B sequence contains a 2082 bp ORF encoding 693 amino acids. The protein molecular weight is 79.1 kD and pI is 8.13. Analysis indicated that RpSTAT5B is a non-transmembrane protein and a non-secreted protein. Homology and evolutionary analysis indicated that RpSTAT5B was closely related to Rhopalosiphum maidis.Conclusions: Unigene expression analysis showed that the total number of differentially expressed genes (DEGs) in the vector aphids was larger than that in the non-vector aphids. Functional enrichment analysis showed that the DEGs related to immunity, growth and reproduction in vector aphids were larger than those in non-vector aphids, and the differentially expressed genes related to immune were up-regulated. This study provides a basis for the evaluation of the response mechanisms of vector/non-vector insects to plant viruses.
Local delivery of USC-derived Exosome Carrying ANGPTL3 Enhance Spinal Cord Functional Recovery After Injury by Promoting Angiogenesis
Authors: Yan Xu , Cao Yong , Chen Chunyuan , Xie Hui , Lu Hongbin , hu jianzhong
Published: July 2020

Background: Spinal cord injury (SCI) is a devastating clinical diseasewithout effectivetherapeuticapproach recently. In this study, we aim to investigate the effect of locally injection with exosome derived human urine stem cell (USC) embedding with hydrogelcould improve the spinal cord functional recovery after injury and the underlying mechanism.Methods:Exosome were isolate from USC andidentified by transmission electron microscopy and western blot. Functional assays using human umbilical vein endothelial cell (HUVEC) in vitro were performed to assess the effects of USC-Exosdeliverythe angiopoietin-like protein 3 (ANGPTL3) on tube formation and migration as well as their regulatory role in the PI3K/AKT signaling pathway activation. In vivo experiment we locally injection with exosome derived USC embedding with hydrogel for treatment of SCI. The effects of USC-Exos on functional recovery in spinal cord injury mice were tested by measuring motor evoked potential, histological and neovascular numbers. Meanwhile, the role of the candidate protein ANGPTL3 in USC-Exo for promoting angiogenesisin SCI was assessed.Results:In current study, we demonstrate that when given locallyinjection with exosomederivedhuman urine stem cell (USC) embeddingwith hydrogelcould pass the spinal cord blood brain barrier and delivery the angiopoietin-like protein 3 (ANGPTL3) to the injured spinal cord region. In addition, the administration of exosome derived from human USC could enhance spinal cord neurological functional recovery by promoting angiogenesis.The mechanism studies revealed that ANGPTL3 are enriched in USCexosome(USC-Exo) and required for USC exosome promoting angiogenesis. Functional studies further confirmed the effects caused by exosome derived from USC on angiogenesis wasmediated by PI3K/AKT signaling pathway. Conclusion:Collectively, our results indicated that USC derived exosome serve as a critical regulator of angiogenesis by transferring ANGPTL3 and may represent a promising novel therapeutic agent for SCI repair.
Spheres of Legislation: Polarization and Most Influential Nodes in Behavioral Context
Authors: Phillips Andrew C , Irfan Mohammad T , Ostertag-Hill Luca
Published: July 2020

Game-theoretic models of influence in networks often assume the network structure to be static. In this paper, we allow the network structure to vary according to the underlying behavioral context. This leads to several interesting questions on two fronts. First, how do we identify different contexts and learn the corresponding network structures using real-world data? We focus on the U.S. Senate and apply unsupervised machine learning techniques, such as fuzzy clustering algorithms and generative models, to identify spheres of legislation as context and learn an influence network for each sphere. Second, how do we analyze these networks in order to gain an insight into the role played by the spheres of legislation in various interesting constructs like polarization and most influential nodes? To this end, we apply both game-theoretic and social network analysis techniques. In particular, we show that game-theoretic notion of most influential nodes brings out the strategic aspects of interactions like bipartisan grouping, which structural centrality measures fail to capture.
Clinical and Genetic Characteristics and Prenatal Diagnosis of Rare Monogenic Global Developmental Delay and Intellectual Disability.
Authors: Lin Liling , Zhang Ying , Pan Hong , Wang Jingmin , Qi Yu , Ma Yinan
Published: July 2020

Background: The estimated worldwide prevalence of global developmental delay (GDD) and intellectual disability (ID) is 1-3%. Rare monogenic GDD/ID is poorly characterized because its low prevalence limits research. In this study, we aimed to describe the diagnostic courses and clinical and genetic characteristics of a cohort with rare monogenic GDD/ID.Method:We retrospectively analyzed the diagnostic courses, clinical characteristics, and genetic spectra of rare monogenic GDD/ID patients. We also conducted a follow-up study on prenatal diagnosis in these families. Mutation pathogenicity was interpreted by molecular geneticists and clinicians according to the guidelines of the American College of Medical Genetics and Genomics. Results:Among 108 patients with rare monogenic GDD/ID, it often took 0.5-4 years and 3-5 referrals to obtain a genetic diagnosis after disease onset. Onset typically occurred before 6 years of age, and patients usually presented moderate to severe GDD/ID. The most common coexisting conditions were epilepsy (68%), facial dysmorphism (14%) and microcephaly (13%). In total, 149 different pathogenic variants were found in 81 different genes among the 108 pedigrees, and 71 variants were novel. The most common inheritance patterns in this outbred Chinese population were autosomal recessive (AR; 46.3%), autosomal dominant (AD; 37%), and X-linked (XL; 16.7%). GLB1, PLA2G6, SCN2A, SHANK3 and STXBP1 were important causal genes. Hot-spot mutations were rarely found. By the follow-up, 43 families, including 24 ARID, 13 ADID and 6 XLID families, had undergone prenatal diagnosis. The offspring of 6 ARID, 2 ADID and 2 XLID families had the same pathogenic variants as the probands.Conclusion:Rare monogenic GDD/ID is characterized by early onset, relatively severe symptoms, great clinical variability and genetic heterogeneity. Moreover, timely referrals to genetic counseling and prenatal diagnostic laboratories are important for affected families planning to have additional children.
Description of Allocanariomyces and Parachaetomium, two new genera, and Achaetomium aegilopis sp. nov. in the Chaetomiaceae
Authors: Mehrabi Mehdi , Asgari Bita , Zare Rasoul
Published: July 2020

We describe Allocanariomyces tritici gen et sp. nov. and Achaetomium aegilopis sp. nov. as seed endophytes of wheat and its poaceous relatives in the west and northwestern provinces of Iran using morphological traits and sequences of ITS region, partial LSU rDNA, β-tubulin and the second largest subunit of DNA-directed RNA polymerase II genes. Chaetomium iranianum, C. truncatulum and C. carinthiacum are also combined here under the new genus, Parachaetomium. Allocanariomyces is differentiated from the closest genus, Canariomyces by having solitary, glabrous perithecia with walls of textura epidermoidea, stalked asci, densely granular-ornamented ascospores with a distinct subapical germ pore, and producing only solitary conidia. Parachaetomium has fusiform or navicular ascospores not bilaterally flattened, compared to Chaetomium with limoniform to globose, bilaterally flattened ascospores. Achaetomium aegilopis is mainly distinguished from A. strumarium, the closest relative, by possessing brown, often scattered perithecia, hyaline perithecial hairs covered with many hyaline crystals, hyaline chlamydospores, and lacking of the asexual morph.
Identification of Key Genes and Pathways in Breast Cancer Based on Bioinformatic Analysis
Authors: Pan Chi , Ni Qingtao
Published: July 2020

Breast cancer(BC) is the most frequent cancer type in women. However, the pathogenesis of BC is still not well understood. Thus, we aim to explore key genes and pathways in relation to BC. We used the Gene Expression Omnibus (GEO) database to identify the differently expression of genes in the carcinogenesis and progression of BC. Then, bioinformatics analysis was performed to determine the key genes targets and pathways associated with BC. The gene expression profile of the hub genes in human tumor was displayed using GEPIA. Finally, the expression of hub genes, correlation between genes and miRNA were screened via the miRDB, MirTarBase and DIANA Tools. We screened 159 downregulated genes and 55 upregulated genes in BC patients among the 4 datasets. The enriched functions of the DEGs involved in cell proliferation, positive regulation of Akt signaling and extracellular exosome, PPAR signaling pathway and AMPK signaling pathway. 3 hub genes were screened out by construction of PPI network. MELK were found to be upregulated, and CLU and NTRK2 were downregulated. Further verification showed that MELK displayed higher levels in almost all tumors. We found correlation between these hube genes and the miRNAs. All in all, three key genes closely related to the incidence of BC were identified, and the results could provide new potential molecular targets for the diagnosis and treatment of BC. In particular, MELK is regulated by multiple miRNAs and participate in the development of BC.
Is the Glass Half-Full or Half-Empty? A Content Analysis of Adolescent Sexual and Reproductive Health in Global Financing Facility Country Plans
Authors: George Asha S , Jacobs Tanya , Kinney Mary V , Haakenstad Annie , Singh Neha , Rasanathan Kumanan , Chopra Mickey
Published: July 2020

Background: The Global Financing Facility (GFF) offers an opportunity to close the financing gap that holds back gains in women, children’s and adolescent health is recognised as a key priority by the GFF leadership. However, very little work exists examining GFF practice. As momentum builds for the GFF, we examine the initial efforts of the GFF in addressing adolescent health.Methods: We undertook a content analysis of the first 10 GFF Investment Cases and Project Appraisal Documents available on the GFF website. The countries involved include Bangladesh, Cameroon, Democratic Republic of Congo, Ethiopia, Guatemala, Kenya, Liberia, Mozambique, Nigeria, Tanzania and Uganda. Results: While several country documents signal understanding and investment in adolescent health as a strategic area, this is not consistent across all countries, nor between Investment Cases and Project Appraisal Documents. In both types of documents commitments weaken as one moves from programming content to indicators to investment. Important contributions include how teenage pregnancy is a universal concern, how adolescent and youth friendly health services and school-based programs are supported in several country documents, how gender is noted as a key social determinant critical for mainstreaming across the health system, alongside the importance of multi-sectoral collaboration, and the acknowledgement of adolescent rights. Weaknesses include the lack of comprehensive analysis of adolescent health needs, inconsistent investments in adolescent friendly health services and school based programs, missed opportunities in not supporting multi-component and multi-level initiatives to change gender norms involving adolescent boys in addition to adolescent girls, and neglect of governance approaches to broker effective multi-sectoral collaboration, community engagement and adolescent involvement. Conclusion: There are important examples of how the GFF supports adolescent health as a priority area. However, more can be done. While building on service delivery approaches more consistently, it must also fund initiatives that address the main social and systems drivers of adolescent health. This requires capacity building for the technical aspects of adolescent health, but also engaging politically to ensure that the right actors are convened to prioritize adolescent health in country plans and to ensure accountability in the GFF process itself.
Development of Integration Indexes to Determine the Extent of Family Planning and Child Immunization Services Integration in Health Facilities in Urban Areas of Nigeria
Authors: Sheahan Kate , Orgill-Meyer Jennifer , Speizer Ilene , Curtis Sian , Paul John , Weinberger Morris , Bennett Antonia V.
Published: July 2020

Background: Integrating family planning into child immunization services may address unmet need for contraception by offering family planning information and services to postpartum women during routine child immunization visits. However, policies and programs promoting integration are often based on insubstantial or conflicting evidence about its effects on service delivery and health outcomes. Since most studies measure integration as binary (a facility is integrated or not) rather than a multidimensional and varying continuum, it is difficult to understand the determinants and effects of integration. This study creates Facility and Provider Integration Indexes, which measure capacity to support integrated family planning and child immunization services, and applies them to analyze the extent of integration across 400 health facilities. Methods: This study utilizes cross-sectional health facility (N= 400; 49% hospitals, 51% primary healthcare centers) and healthcare provider (N= 1,479) survey data that were collected in six urban areas of Nigeria for the impact evaluation of the Nigerian Urban Reproductive Health Initiative. Principal Components Analysis was used to develop Provider and Facility Integration Indexes that estimate the extent of integration in these health facilities. The Provider Integration Index measures provider skills and practices that support integrated service delivery while the Facility Integration Index measures facility norms that support integrated service delivery. Index scores range from zero (low) to ten (high). Results: Mean Provider Integration Index score is 5.42 (SD 3.10), and mean Facility Integration Index score is 6.22 (SD 2.72). Twenty-three percent of facilities were classified as having low Provider Integration scores, 32% as medium, and 45% as high. Fourteen percent of facilities were classified as having low Facility Integration scores, 38% as medium, and 48% as high. Conclusion: Many facilities in our sample have achieved high levels of integration, while many others have not. Results suggest that measuring integration as a binary variable does not (a) reflect the true variation in integration within and across health facilities, (b) enable nuanced measurement of the determinants or effects of integration, or (c) provide tailored, actionable information about how best to improve integration. Overall, results reinforce the importance of utilizing more nuanced measures of facility-level integration.
Mesenchymal stem cells regulate activation of microglia cells to improve hippocampal nerve damage of heat stroke rats
Authors: Wang Lu , Zhao Yan , Deng Zihui , Yuan Rui , Yang Mengmeng , Zhang Yu , Li Yun , Zhou Feihu , Kang Hong-Jun
Published: July 2020

Background

Heat stroke (HS) is a severe systemic inflammatory response disease caused by high fever, mainly with nervous system damage. Currently, mesenchymal stem cells (MSCs) have inflammation and immunomodulatory effects. Therefore, we aimed to explore the protective effect and mechanism of MSCs on HS-induced excessive inflammation and neurological dysfunction.
Methods

A heat stroke Sprague-Dawley (SD) rat model was established at continuous high temperature (42 °C) and high humidity (70%-80%). After modeling, the rats were randomly divided into a heat stroke model group (HS group), a MSCs treatment group (HS + MSCs group), and a Control group in which the rats were kept at room temperature without any treatment. Survival analysis, neurological deficit scoring, pathological staining of hippocampus and cerebellum, immunofluorescence staining of microglia cells, and tissue level detection of inflammatory cytokines were performed in the three groups on day 1, 3, 7, 14 and 28 separately.
Results

After heat stroke modeling, the rats were severely paralyzed and had a high mortality. MSCs treatment significantly reduced the mortality in both early stage (day 3) and late stage (day 28). MSCs treatment also significantly reduced the neurological impairment of heat stroke rats, and improved hippocampal and cerebellar pathology and neuronal cell damage. In addition, MSCs treatment significantly inhibited the overactivation of microglia in the hippocampus of HS rats as well as the levels of pro-inflammatory factors and chemokines in the hippocampus. In the early stage (day 1) of MSCs treatment, the activation of cerebellar microglia in the heat stroke rats was significantly promoted. Meanwhile, MSCs treatment had no significant inhibitory effect on the levels of pro-inflammatory factors in the cerebellar tissues of heat stroke rats, but can inhibit the levels of chemokines in the early stage.
Conclusions

The application of MSCs for heat stroke treatment in rats can significantly reduce the mortality and neurological defects and improve the hippocampal injury. Meanwhile, MSCs can inhibit the over-activation of microglia cells in the hippocampus of heat stroke rats, which may be a mechanism of MSCs in protecting heat-stroke-caused hippocampal injury.