bluuebunny/crossref_metadata_2025 huggingface dataset:
Adjustments to Proactive Motor Inhibition without Effector-Specific Foreknowledge Are Reflected in a Bilateral Upregulation of Sensorimotor β-Burst Rates
Authors: Soh Cheol , Hynd Megan , Rangel Benjamin O. , Wessel Jan R.
Published: February 2021

Classic work using the stop-signal task has shown that humans can use inhibitory control to cancel already initiated movements. Subsequent work revealed that inhibitory control can be proactively recruited in anticipation of a potential stop-signal, thereby increasing the likelihood of successful movement cancellation. However, the exact neurophysiological effects of proactive inhibitory control on the motor system are still unclear. On the basis of classic views of sensorimotor β-band activity, as well as recent findings demonstrating the burst-like nature of this signal, we recently proposed that proactive inhibitory control is implemented by influencing the rate of sensorimotor β-bursts during movement initiation. Here, we directly tested this hypothesis using scalp EEG recordings of β-band activity in 41 healthy human adults during a bimanual RT task. By comparing motor responses made in two different contexts—during blocks with or without stop-signals—we found that premovement β-burst rates over both contralateral and ipsilateral sensorimotor areas were increased in stop-signal blocks compared to pure-go blocks. Moreover, the degree of this burst rate difference indexed the behavioral implementation of proactive inhibition (i.e., the degree of anticipatory response slowing in the stop-signal blocks). Finally, exploratory analyses showed that these condition differences were explained by a significant increase in β bursting that was already present during baseline period before the movement initiation signal. Together, this suggests that the strategic deployment of proactive inhibitory motor control is implemented by upregulating the tonic inhibition of the motor system, signified by increased sensorimotor β-bursting both before and after signals to initiate a movement.
Behavioral and Neural Fusion of Expectation with Sensation
Authors: Panichello Matthew F. , Turk-Browne Nicholas B.
Published: February 2021

Humans perceive expected stimuli faster and more accurately. However, the mechanism behind the integration of expectations with sensory information during perception remains unclear. We investigated the hypothesis that such integration depends on “fusion”—the weighted averaging of different cues informative about stimulus identity. We first trained participants to map a range of tones onto faces spanning a male–female continuum via associative learning. These two features served as expectation and sensory cues to sex, respectively. We then tested specific predictions about the consequences of fusion by manipulating the congruence of these cues in psychophysical and fMRI experiments. Behavioral judgments and patterns of neural activity in auditory association regions revealed fusion of sensory and expectation cues, providing evidence for a precise computational account of how expectations influence perception.
Effects of Temporal Expectations on the Perception of Motion Gestalts
Authors: Nobre Alexandre de P. , Nikolaev Andrey R. , Gauer Gustavo , van Leeuwen Cees , Wagemans Johan
Published: February 2021

Gestalt psychology has traditionally ignored the role of attention in perception, leading to the view that autonomous processes create perceptual configurations that are then attended. More recent research, however, has shown that spatial attention influences a form of Gestalt perception: the coherence of random-dot kinematograms (RDKs). Using ERPs, we investigated whether temporal expectations exert analogous attentional effects on the perception of coherence level in RDKs. Participants were presented fixed-length sequences of RDKs and reported the coherence level of a target RDK. The target was indicated immediately after its appearance by a postcue. Target expectancy increased as the sequence progressed until target presentation; afterward, remaining RDKs were perceived without target expectancy. Expectancy influenced the amplitudes of ERP components P1 and N2. Crucially, expectancy interacted with coherence level at N2, but not at P1. Specifically, P1 amplitudes decreased linearly as a function of RDK coherence irrespective of expectancy, whereas N2 exhibited a quadratic dependence on coherence: larger amplitudes for RDKs with intermediate coherence levels, and only when they were expected. These results suggest that expectancy at early processing stages is an unspecific, general readiness for perception. At later stages, expectancy becomes stimulus specific and nonlinearly related to Gestalt coherence.
Scenarios as Tools of the Scientific Imagination: The Case of Climate Projections
Authors: Poznic Michael , Hillerbrand Rafaela
Published: February 2021

Climatologists have recently introduced a distinction between projections as scenario-based model results on the one hand and predictions on the other hand. The interpretation and usage of both terms is, however, not univocal. It is stated that the ambiguities of the interpretations may cause problems in the communication of climate science within the scientific community and to the public realm. This paper suggests an account of scenarios as props in games of make-belive. With this account, we explain the difference between projections that should be make-believed and other model results that should be believed.
Stepping Forwards by Looking Back: Underdetermination, Epistemic Scarcity and Legacy Data
Authors: Currie Adrian
Published: February 2021

Debate about the epistemic prowess of historical science has focused on local underdetermination problems generated by a lack of historical data; the prevalence of information loss over geological time, and the capacities of scientists to mitigate it. Drawing on Leonelli’s recent distinction between ‘phenomena-time’ and ‘data-time’ I argue that factors like data generation, curation and management significantly complexifies and undermines this: underdetermination is a bad way of framing the challenges historical scientists face. In doing so, I identify circumstances of epistemic scarcity where underdetermination problems are particularly salient, and discuss cases where legacy data—data generated using differing technologies and systems of practice—are drawn upon to overcome underdetermination. This suggests that one source of overcoming underdetermination is our knowledge of science’s past. Further, data-time makes agnostic positions about the epistemic fortunes of scientists working under epistemic scarcity more plausible. But agnosticism seems to leave philosophers without much normative grip. So, I sketch an alternative approach: focusing on the strategies scientists adopt to maximize their epistemic power in light of the resources available to them.
Multiomic Profiling of Iron Deficient Infant Monkeys Detects Biochemical Signatures of Inflammatory and Neural Dysfunction in Blood and CSF Prior to Anemia
Authors: Sandri Brian , Lubach Gabriele , Lock Eric , Kling Pam , Georgieff Michael , Coe Christopher , Rao Raghavendra
Published: February 2021

Background: Pre-anemic iron deficiency (ID) and ID anemia in infancy is associated with long-term effects on brain development. Iron is an essential micronutrient needed for multiple neurodevelopmental processes, including energy production, myelination and neurotransmission. Serum and cerebrospinal fluid (CSF) metabolomic and proteomic analyses can potentially characterize the metabolic and protein signatures of aberrant iron-dependent brain development pathways. A better understanding of the timing and course of the biochemical signatures of ID-induced dysfunction in the heme and intrathecal compartments may help with early diagnosis and treatment of iron deficiency prior to onset of anemia.

Objective: To determine proteomic and metabolomic signatures of ID-induced dysfunction in serum and CSF of infant monkeys prior to the onset of iron deficiency anemia (IDA).

Methods: Hematological parameters were determined in rhesus monkey infants (Macaca mulatta) at 4 and 6 months of age as they progressed from preanemic ID to IDA (ID group; n=6) or remained iron-sufficient(IS group; n=12). Blood and CSF were collected at 4 and 6 months and metabolically profiled using HPLC/MS with isobaric standards for identification and quantification. Proteomic quantification utilized a custom TMT-labeled workflow with an Orbitrap Fusion Tribrid mass spectrometer. Serum and CSF metabolomic and proteomic profiles of the ID and IS groups were compared, along with a a third group of infants that became IDA earlier at 4 months of age (IDA group; n=3).

Results: Evidence of pre-anemic ID was apparent in some infant at 4 months of age with clinical IDA evident at 6 months A (Hgb <10 g/dL and MCV <60 fL). A total of 1,128 CSF proteins and 251 serum proteins were identified and quantified. Over 4,000 metabolite features were observed in serum and CSF across three modalities including reverse phase positive, reverse phase negative and HILIC mode. Pathway analyses provided evidence of increased inflammatory activity, impacts on neural pathways, and bioindicators of acute phase responses in ID and IDA groups. Signs of these metabolic changes were already present in the preanemic ID? period (4 months).

Conclusions: Biomarkers indicating indicative of increased inflammatory activity with downstream effects on pathways involved in normal neural function were detectable in both the CSF and serum of ID monkeys prior to the onset of clinical anemia. Brain-specific proteomic and metabolomic profiling of novel analytes in circulation can facilitate earlier diagnosis and treatment of infantile ID to reduce neurodevelopmental risk.

Funded by NIH/NICHD Grant HD089989, this abstract is sponsored by ASH member Zohar Sachs MD, PhD

Disclosures

No relevant conflicts of interest to declare.
Effects of MiR-451a on the Phagocytosis and Polarization of Macrophages
Authors: Liu Xiaoli , Zhang Dongyue , Wang Hao , Ren Qian , Wang Lina , Yuan Weiping , Zheng Guoguang
Published: February 2021

Macrophages are important member in tissue microenvironments and play diverse physiologic and pathologic roles. Leukemia associated macrophages (LAM) are a kind of specifically activated macrophages in leukemia microenvironment, which are different from M1, M2 and TAMs. We have reported the heterogeneities in gene expression profiles of LAMs. However, MicroRNA expression profiles of LAMs and regulatory mechanism are still unknown. Here, a MLL-AF9 induced mouse acute myeloid leukemia (AML) model was used, and LAMs in the spleen and bone marrow were sorted for microRNA sequencing. The microRNA expression profiles of LAMs in bone marrow and spleen in AML mice were different from macrophages from control mice. Based on the volcano plot, more than 100 microRNAs were differentially expressed in LAMs compared with macrophages in control mice. Next, five differentially expressed microRNAs were selected and verified by qRT-PCR in LAMs from spleen. The results showed that miR-451a and miR-155-5p in spleen LAMs were significantly upregulated in LAMs from spleen. Overexpression of miR-451a altered the morphology of macrophages, enhanced the phagocytic ability of macrophages, and promotes the expression of macrophage differentiation marker CD11b. Furthermore, overexpression of miR-451a had little effect on M0 macrophages, but increased the proliferation capacity of macrophages upon stimulation toward M1 or M2 phenotype. MiR-451a overexpressed-macrophages had higher level of iNOS when stimulated with LPS or IL-4 whereas there was no difference in the expression of IL-1β, IL-6, CD206 and Arg-1 between MiR-451a overexpressed-macrophages and control macrophage. Therefore, our data revealed the characteristics of the microRNA expression profile of LAMs for the first time, and verified the effect of miR-451a on macrophage in vitro.

Disclosures

No relevant conflicts of interest to declare.
Fossil oceanic core complexes in the Alps. New field, geochemical and isotopic constraints from the Tethyan Aiguilles Rouges Ophiolite (Val d’Hérens, Western Alps, Switzerland)
Authors: Decrausaz Thierry , Müntener Othmar , Manzotti Paola , Lafay Romain , Spandler Carl
Published: February 2021

Exhumation of basement rocks on the seafloor is a worldwide feature along passive continental margins and (ultra-) slow-spreading environments, documented by dredging, drilling or direct observations by diving expeditions. Complementary observations from exhumed ophiolites in the Alps allow for a better understanding of the underlying processes. The Aiguilles Rouges ophiolitic units (Val d’Hérens, Switzerland) are composed of kilometre-scale remnants of laterally segmented oceanic lithosphere only weakly affected by Alpine metamorphism (greenschist facies, Raman thermometry on graphite: 370–380 °C) and deformation. Geometries and basement-cover sequences comparable to the ones recognized in actual (ultra-) slow-spreading environments were observed, involving exhumed serpentinized and carbonatized peridotites, gabbros, pillow basalts and tectono-sedimentary cover rocks. One remarkable feature is the presence of a kilometric gabbroic complex displaying preserved magmatic minerals, textures and crosscutting relationships between the host gabbro and intruding diabase, hornblende-bearing dikelets or plagiogranite. The bulk major and trace element chemistry of mafic rocks is typical of N-MORB magmatism (CeN/YbN: 0.42–1.15). This is supported by in-situ isotopic signatures of magmatic zircons (εHf =  + 13 ± 0.6) and apatites (εNd =  + 8.5 ± 0.8), determined for gabbros and plagiogranites. In-situ U–Pb dating was performed on zircons by laser ablation-ICP-MS, providing ages of 154.9 ± 2.6 Ma and 155.5 ± 2.8 Ma, which are among the youngest for oceanic gabbros in the Alps. Our study suggests that the former Aiguilles Rouges domain was characterized by tectonism and magmatism resembling present-day (ultra-) slow-spreading seafloor. It also suggests that the Tethyan lithosphere is laterally segmented, with punctuated magmatism such as the Aiguilles Rouges gabbros and carbonated ultramafic seafloor covered by basalts and Jurassic tectono-sedimentary deposits.
Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
Authors: Moran-Bertot Ivis , Rodríguez-Cabrera Lianet , Borras-Hidalgo Orlando , Huang Siliang , Kan Yunchao , Wright Denis J. , Ayra-Pardo Camilo
Published: February 2021

Background

The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae.

Results

The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls.

Conclusions

We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.
The STS case study: an analysis method for longitudinal qualitative research for implementation science
Authors: Van Tiem Jennifer M. , Schacht Reisinger Heather , Friberg Julia E. , Wilson Jaime R. , Fitzwater Lynn , Panos Ralph J. , Moeckli Jane
Published: February 2021

Background

Ethnographic approaches offer a method and a way of thinking about implementation. This manuscript applies a specific case study method to describe the impact of the longitudinal interplay between implementation stakeholders. Growing out of science and technology studies (STS) and drawing on the latent archaeological sensibilities implied by ethnographic methods, the STS case-study is a tool for implementors to use when a piece of material culture is an essential component of an innovation.

Methods

We conducted an ethnographic process evaluation of the clinical implementation of tele-critical care (Tele-CC) services in the Department of Veterans Affairs. We collected fieldnotes and conducted participant observation at virtual and in-person education and planning events (n = 101 h). At Go-Live and 6-months post-implementation, we conducted site visits to the Tele-CC hub and 3 partnered ICUs. We led semi-structured interviews with ICU staff at Go-Live (43 interviews with 65 participants) and with ICU and Tele-CC staff 6-months post-implementation (44 interviews with 67 participants). We used verification strategies, including methodological coherence, appropriate sampling, collecting and analyzing data concurrently, and thinking theoretically, to ensure the reliability and validity of our data collection and analysis process.

Results

The STS case-study helped us realize that we must think differently about how a Tele-CC clinician could be noticed moving from communal to intimate space. To understand how perceptions of surveillance impacted staff acceptance, we mapped the materials through which surveillance came to matter in the stories staff told about cameras, buttons, chimes, motors, curtains, and doorbells.

Conclusions

STS case-studies contribute to the literature on longitudinal qualitive research (LQR) in implementation science, including pen portraits and periodic reflections. Anchored by the material, the heterogeneity of an STS case-study generates questions and encourages exploring differences. Begun early enough, the STS case-study method, like periodic reflections, can serve to iteratively inform data collection for researchers and implementors. The next step is to determine systematically how material culture can reveal implementation barriers and direct attention to potential solutions that address tacit, deeply rooted challenges to innovations in practice and technology.
Correction to: Sperm parameters and anti-Müllerian hormone remain stable with Helicobacter pylori infection: a cross-sectional study
Authors: Feng Chun , Lv Ping-Ping , Huang Chang-Chang , Yang Song-Qing , Yao Qiu-Ping , Shen Jin-Ming , Jin Min
Published: February 2021

An amendment to this paper has been published and can be accessed via the original article.
The effect of family-based counseling on domestic violence in pregnant women referring to health centers in Sahneh city, Iran, 2018
Authors: Babaheidarian Fereshteh , Masoumi Seyedeh Zahra , Sangestani Gita , Roshanaei Godratollah
Published: February 2021

Background

Domestic violence is a term that shows aggressive behavior with different physical, psychological, economic, and social dimensions. This concept is considered as one of the health priorities regarding its significant effects on pregnancy, postpartum, and the outcome of pregnancy. The present study was conducted to investigate the effect of family-based counseling on domestic violence against pregnant women.

Methods

An intervention trial was conducted on 92 pregnant women exposed to domestic violence, selected among 274 pregnant women accessible in Sahneh, Iran. Data collection was performed using the standard questionnaire of domestic violence against women of Mohseni Tabrizi. The pregnant women exposed to violence were randomly assigned to intervention and control groups (in two groups of 45 people). In the intervention group, three 45-min individual counseling sessions were held for the pregnant women and their spouses according to Greeting, Ask, Tell, Help, Explain, and Refer (GATHER) principles. Four weeks after the end of the sessions, the two groups again completed the questionnaire.

Results

Family-based counseling intervention reduced the mean score of domestic violence in the intervention group from 68.58 ± 9.21 before the intervention to 49.56 ± 8.83 after intervention. Also, various domains of violence including mental, verbal, financial, physical, sexual, and social violence were significantly declined in the intervention group (P < 0.001); however, there was no significant decrease in emotional violence score (P > 0.001).

Conclusion

Family-based counseling plays a significant role in reducing the various types of violence against women through increasing the awareness of couples and by improving the relationship between couples during pregnancy. It will reduce the incidence of violence against a pregnant woman and consequently reduce complications on the mother and pregnancy outcomes. Family-oriented counseling played a significant role in deterring all forms of violence against women by increasing awareness of couples and improving their relationship during pregnancy. Moreover, family-oriented counseling reduced the incidence of violence against pregnant women and thus deterred maternal complications associated with pregnancy.
Correction to: Assessing responsiveness of the EQ-5D-3L, the Oxford Hip Score, and the Oxford Knee Score in the NHS patient-reported outcome measures
Authors: Kang Sujin
Published: February 2021

An amendment to this paper has been published and can be accessed via the original article.
Effect of 150 kHz electromagnetic radiation on the development of polycystic ovaries induced by estradiol Valerate in Sprague Dawley rats
Authors: Mohammed Stephanie , Sundaram Venkatesan , Zyuzikov Nikolay
Published: February 2021

Background

Polycystic ovary syndrome (PCOS) is the most common complex endocrine disorder affecting approximately 2–20% of reproductive aged females. Tumour Treating Fields (100–300 kHz) is a recent innovative, non-invasive therapeutic approach to cancer therapy. This frequency as an alternative therapy for the management of polycystic ovaries has not yet been explored.

Objectives

To explore the effect of full-body exposure of 150 kHz Electromagnetic Radiation (EMR), on the development of polycystic ovaries in an estradiol valerate-induced PCO rat model.

Method

Twenty-one female adult rats were divided into three groups (n = 7 each): control, Estradiol Valerate (EV) and EV + EMR groups. The EV + EMR group was subjected to full body exposure at 150 kHz EMR continuously for eight consecutive weeks. Estradiol valerate was administered orally to induce polycystic ovaries in EV and EV + EMR groups. Body and ovarian weights were recorded and analysed. The regularity of the estrous cycle was assessed in all three groups. The histological study of ovarian tissue was carried out by haematoxylin and eosin staining. The serum concentration levels of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH) and testosterone were measured using the ELISA method.

Results

The body and ovary weights did not differ significantly between the EV and EV + EMR groups. The estrous cycle was found to be irregular in both the EV and EV + EMR groups. Ovarian histology revealed near normal morphology with little or no degenerative and morphological changes in developing follicles in the exposed group. Histometrical analysis showed an increased number of developing follicles and a significant reduction in the number and size of follicular cysts (p < 0.05) in the EV + EMR group. Hormonal analysis revealed no significant difference in the testosterone and FSH levels between the EV + EMR and EV groups. However, the LH, LH/FSH ratio decreased significantly in the EV + EMR group compares to the EV group.

Conclusion

The 150 kHz EMR appear to have little or no degenerative and morphological changes in the developing follicles, an increased number of typical developing follicles and a significant reduction in the number and size of the follicular cysts (p < 0.05).
Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H2O2-induced oxidative damage in RAW 264.7 cells
Authors: Huang Qi , He Wen , Khudoyberdiev Ilkhomjon , Ye Chun-Lin
Published: February 2021

This work presents an investigation on the composition and structure of polysaccharides from the roots of Tetrastigma hemsleyanum (THP) and its associated antioxidant activity. It further explores the protective effect of THP on RAW264.7 cells against cytotoxicity induced by H2O2. Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. Furthermore, gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS) was employed to deduce the relative molecular mass (Mw) of the polysaccharide, which was 177.1 ± 1.8 kDa. Fourier transform infrared spectroscopy (FT-IR) and Congo red binding assay highlighted that the THP had a steady α-triple helix conformation. Similarly, assays of antioxidant activity disclosed that THP had reasonable concentration-dependent hydroxyl radical and superoxide radical scavenging activities, peroxidation inhibition ability and ferrous ion chelating potency, in addition to a significant 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity. Moreover, THP could protect RAW264.7 cells against H2O2-induced cytotoxicity by decreasing intracellular ROS levels, reducing catalase (CAT) and superoxide dismutase (SOD) activities, increasing lactate dehydrogenase (LDH) activity and increment in malondialdehyde (MDA) level. Data retrieved from the in vitro models explicitly established the antioxidant capability of polysaccharides from T. hemsleyanum root extracts.
Implementing genomic screening in diverse populations
Authors: Abul-Husn Noura S. , Soper Emily R. , Braganza Giovanna T. , Rodriguez Jessica E. , Zeid Natasha , Cullina Sinead , Bobo Dean , Moscati Arden , Merkelson Amanda , Loos Ruth J. F. , Cho Judy H. , Belbin Gillian M. , Suckiel Sabrina A. , Kenny Eimear E.
Published: February 2021

Background

Population-based genomic screening has the predicted ability to reduce morbidity and mortality associated with medically actionable conditions. However, much research is needed to develop standards for genomic screening and to understand the perspectives of people offered this new testing modality. This is particularly true for non-European ancestry populations who are vastly underrepresented in genomic medicine research. Therefore, we implemented a pilot genomic screening program in the BioMeBiobank in New York City, where the majority of participants are of non-European ancestry.

Methods

We initiated genomic screening for well-established genes associated with hereditary breast and ovarian cancer syndrome (HBOC), Lynch syndrome (LS), and familial hypercholesterolemia (FH). We evaluated and included an additional gene (TTR) associated with hereditary transthyretin amyloidosis (hATTR), which has a common founder variant in African ancestry populations. We evaluated the characteristics of 74 participants who received results associated with these conditions. We also assessed the preferences of 7461 newly enrolled BioMeparticipants to receive genomic results.

Results

In the pilot genomic screening program, 74 consented participants received results related to HBOC (N = 26), LS (N = 6), FH (N = 8), and hATTR (N = 34). Thirty-three of 34 (97.1%) participants who received a result related to hATTR were self-reported African American/African (AA) or Hispanic/Latinx (HL), compared to 14 of 40 (35.0%) participants who received a result related to HBOC, LS, or FH. Among the 7461 participants enrolled after the BioMeprotocol modification to allow the return of genomic results, 93.4% indicated that they would want to receive results. Younger participants, women, and HL participants were more likely to opt to receive results.

Conclusions

The addition ofTTRto a pilot genomic screening program meant that we returned results to a higher proportion of AA and HL participants, in comparison with genes traditionally included in genomic screening programs in the USA. We found that the majority of participants in a multi-ethnic biobank are interested in receiving genomic results for medically actionable conditions. These findings increase knowledge about the perspectives of diverse research participants on receiving genomic results and inform the broader implementation of genomic medicine in underrepresented patient populations.
Evidence for Glucagon Secretion and Function Within the Human Gut
Authors: Sun Emily W , Martin Alyce M , de Fontgalland Dayan , Sposato Luigi , Rabbitt Philippa , Hollington Paul , Wattchow David A , Colella Alexander D , Chataway Tim , Wewer Albrechtsen Nicolai J , Spencer Nick J , Young Richard L , Keating Damien J
Published: February 2021

Glucagon is secreted by pancreatic α cells in response to hypoglycemia and increases hepatic glucose output through hepatic glucagon receptors (GCGRs). There is evidence supporting the notion of extrapancreatic glucagon but its source and physiological functions remain elusive. Intestinal tissue samples were obtained from patients undergoing surgical resection of cancer. Mass spectrometry analysis was used to detect glucagon from mucosal lysate. Static incubations of mucosal tissue were performed to assess glucagon secretory response. Glucagon concentration was quantitated using a highly specific sandwich enzyme-linked immunosorbent assay. A cholesterol uptake assay and an isolated murine colonic motility assay were used to assess the physiological functions of intestinal GCGRs. Fully processed glucagon was detected by mass spectrometry in human intestinal mucosal lysate. High glucose evoked significant glucagon secretion from human ileal tissue independent of sodium glucose cotransporter and KATP channels, contrasting glucose-induced glucagon-like peptide 1 (GLP-1) secretion. The GLP-1 receptor agonist Exendin-4 attenuated glucose-induced glucagon secretion from the human ileum. GCGR blockade significantly increased cholesterol uptake in human ileal crypt culture and markedly slowed ex vivo colonic motility. Our findings describe the human gut as a potential source of extrapancreatic glucagon and demonstrate a novel enteric glucagon/GCGR circuit with important physiological functions beyond glycemic regulation.
ПСИХОЛОГИЧЕСКИЕ ФАКТОРЫ ВЕРОЯТНОСТНЫХ ЛИЧНОСТНЫХ РАССТРОЙСТВ
Authors: Kazieva A. T. , Bibilova Z. L. , Khudalova M. Z.
Published: February 2021

Вследствие распространения нарушений адаптации и аутоидентификации в современном обществе, а так же,противоречивой информации о факторах возникновения личностных расстройств, нас заинтересовал вопрос о связи данного расстройства с индивидуально-психологическими особенностями личности. Для изучения этой проблемы нами осуществлено исследование с привлечением 40 человек обоих полов и разного возраста, в котором применены методики для диагностики личностных черт и степени вероятностного расстройства личности. В результате была обнаружена корреляционная связь между проявлениями вероятностного личностного расстройства и индивидуально-психологическими особенностями респондентов нашей выборки.
Ultrashort pulses from a 8 μm wavelength semiconductor laser
Authors: Hillbrand Johannes , Opačak Nikola , Piccardo Marco , Schneider Harald , Strasser Gottfried , Capasso Federico , Schwarz Benedikt
Published: February 2021

The ultrafast carrier transport in mid-infrared QCLs has so far constituted a seemingly insurmountable obstacle for the formation of short pulses. Here, we demonstrate transformation-limited picosecond pulses generated by 8 μm wavelength QCLs at room-temperature.
Manipulation of multipartite entanglement in an array of quantum dots
Authors: Chen Yen-Ju , Chuu Chih-Sung
Published: February 2021

Multipartite entanglement is indispensable in the implementation of quantum technologies and the fundamental test of quantum mechanics. Here we study how the W state and W-like state may be generated in a quantum-dot array by controlling the coupling between an incident photon and the quantum dots on a waveguide. We also discuss how the coupling may be controlled to observe the sudden death of entanglement. Our work can find potential applications in quantum information processing.
Harmonic generation from gold nanolayers: bound and hot electron contributions to nonlinear dispersion
Authors: Rodríguez-Suné L. , Trull J. , Cojocaru C. , Akozbek N. , De Ceglia D. , Vincenti M. A. , Scalora M.
Published: February 2021

Understanding how light interacts with matter at the nanoscale is pivotal if one is to properly engineer nano-antennas, filters and other devices whose geometrical features approach atomic size. We report experimental results on second and third harmonic generation from 20 nm- and 70 nm-thick gold layers, for TE- and TM-polarized incident light pulses. We discuss the relative roles that bound electrons and an intensity dependent free electron density (hot electrons) play in third harmonic generation. While planar structures are generally the simplest to fabricate, metal layers that are only a few nanometers thick and partially transparent are almost never studied. Yet, transmission offers an additional reference point to compare experimental measurements with theoretical models. Our experimental results are explained well within the context of the microscopic hydrodynamic model that we employ to simulate second and third harmonic conversion efficiencies. Using our experimental observations we estimate

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Fast refocusing lens based on ferroelectric liquid crystals
Authors: Mukherjee Souptik , Yuan Zheng-nan , Sun Zhi-bo , Li An-ran , Kang Chen-bing , Kwok Hoi-Sing , Srivastava Abhishek K.
Published: February 2021

Optical devices like virtual reality (VR) headsets present challenges in terms of vergence-accommodation conflict that leads to visual fatigue for the user over time. Lenses available to meet these challenges include liquid crystal (LC) lenses, which possess a response time in the millisecond range. This response time is slow, while accessing multiple focal lengths. A ferroelectric liquid crystal (FLC) has a response time in the microsecond range. In this article, we disclose a switchable lens device having a combination of the fast FLC-based polarization rotation unit and a passive polarization-dependent LC lens. A cascaded combination of three such lens units allows access to eight different focal points quite rapidly and can be a convenient device for VR applications.
Dual modal plasmonic substrates based on a convective self-assembly technique for enhancement in SERS and LSPR detection
Authors: Choi Munsik , Kang Taeyoung , Choi Seung Ho , Byun Kyung Min
Published: February 2021

In this study, surface-enhanced Raman scattering (SERS) scheme is combined with localized surface plasmon resonance (LSPR) detection on a thin gold film with stripe patterns of gold nanoparticles (GNPs) via convective self-assembly (CSA) method. The potential of dual modal plasmonic substrates was evaluated by binding 4-ABT and IgG analytes, respectively. SERS experiments presented not only a high sensitivity with a detection limit of 4.7 nM and an enhancement factor of 1.34 × 105, but an excellent reproducibility with relative standard deviation of 5.5%. It was found from plasmonic sensing experiments by immobilizing IgG onto GNP-mediated gold film that detection sensitivity was improved by more than 211%, compared with a conventional bare gold film. Our synergistic SERS–LSPR approach based on a simple and cost-effective CSA method could open a route for sensitive, reliable and reproducible dual modal detection to expand the application areas.
Ultra-compact silicon-microcap based improved Michelson interferometer high-temperature sensor
Authors: Han Yang , Liu Bo , Wu Yongfeng , Mao Yaya , Wu Jing , Zhao Lilong , Nan Tong , Wang Jin , Tang Rong , Zhang Yulan , Liu Yan
Published: February 2021

An ultra-short high-temperature fiber-optic sensor based on a silicon-microcap created by a single-mode fiber (SMF) and simple fusion splicing technology is proposed and experimentally demonstrated. A section of the SMF with a silicon-microcap at one end is connected to the “peanut” structure to build the microcap-based optical fiber improved Michelson interferometer (MI). The optimal discharge parameters of microcap and length of SMF has been investigated to achieve the best extinction ratio of 6.61 dB. The size of this microcap-based improved MI sensor is 560 µm and about 18 times shorter compared to the current fiber tip interferometers (about 10 mm). Meanwhile, it showed good robustness during the two heating-cooling cycles and the duration period stability test at 900 °C. This microcap-based improved MI sensor with the smaller size, simple fabrication, low cost, high reliability, and good linearity within a large dynamic range is beneficial to practical temperature measurement and massive production.
Optical properties of mid-infrared Cr2+:ZnSe single crystals grown by chemical vapor transporting with NH4Cl
Authors: Wei Yucheng , Liu Changyou , Ma En , Wang Tao , Jie Wanqi
Published: February 2021

In the recent two decades, Cr2+:ZnSe crystals have been widely used as a gain media for 2∼3 μm mid-infrared lasers. However, it still remains a huge challenge for researchers to meet more and more requirements on the crystals with high qualities and large sizes. In this work, one Cr2+:ZnSe single crystal with a diameter of about 15 mm was successfully grown by chemical vapor transporting (CVT) with NH4Cl in a closed quartz ampoule without any seed. The transmission of as-grown crystal is up to 70% in the UV-VIS-NIR region and is with an intense characteristic absorption of Cr2+ near 1770 nm. The X-ray photoelectron spectroscopy (XPS) shows that the incorporation of chlorine anions does not significantly affect the valence distribution of components in the crystal. The mid-infrared photoluminescence spectra show a strong and broad emission band centered at 2400 nm with a width of 600 nm under the 1770 nm laser excitation at room temperature. The calculated cross sections of absorption and emission were 1.31×10−18 and 1.4×10−18 cm2, respectively. The measured photoluminescence decay time was about 6.9 μs at room temperature. It is confirmed that the method of CVT with NH4Cl is suitable for the growth of Cr2+:ZnSe single crystals, which are expected to have a promising prospect in mid-infrared laser applications in further.
Neural manifold under plasticity in a goal driven learning behaviour
Authors: Feulner Barbara , Clopath Claudia
Published: February 2021

Neural activity is often low dimensional and dominated by only a few prominent neural covariation patterns. It has been hypothesised that these covariation patterns could form the building blocks used for fast and flexible motor control. Supporting this idea, recent experiments have shown that monkeys can learn to adapt their neural activity in motor cortex on a timescale of minutes, given that the change lies within the original low-dimensional subspace, also called neural manifold. However, the neural mechanism underlying this within-manifold adaptation remains unknown. Here, we show in a computational model that modification of recurrent weights, driven by a learned feedback signal, can account for the observed behavioural difference between within- and outside-manifold learning. Our findings give a new perspective, showing that recurrent weight changes do not necessarily lead to change in the neural manifold. On the contrary, successful learning is naturally constrained to a common subspace.
Human T-cell leukemia virus type 1 infection among Japanese immigrants and their descendants living in Southeast Brazil: A call for preventive and control responses
Authors: Bandeira Larissa M. , Puga Marco A. M. , Weis-Torres Sabrina M. S. , Rezende Grazielli R. , Domingos João A. , Tanaka Tayana S. O. , Cesar Gabriela A. , Nukui Youko , Vicente Ana C. P. , Casseb Jorge , Yamashiro Juliana , Segurado Aluísio C. , Saito Murilo O. , Pinho João R. R. , Cunha Rivaldo V. , Okumoto Osnei , Uehara Silvia N. O. , Motta-Castro Ana R. C.
Published: February 2021

Human T-cell leukemia virus type 1 (HTLV-1) has worldwide distribution and is considered endemic in southwestern Japan. HTLV-1 infection has been associated with adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) besides other diseases. This cross-sectional study aimed to investigate the prevalence, risk factors and molecular characterization of HTLV-1, among the world’s largest population of Japanese immigrants and their descendants outside of Japan, in São Paulo, Southeast Brazil, as well as to analyze the phylogenetic relationship among isolates of HTLV-1. From July to December 2017, 2,139 individuals from five Japanese associations were interviewed and submitted to blood collection. All serum samples were first tested for the presence of anti-HTLV-1/2 antibodies by ELISA and then peripheral blood from individuals with positive serological results were analyzed for the presence of HTLV-1 5’LTR proviral DNA. Partial sequencing of the 5’LTR region of HTLV-1 proviral DNA was performed by Sanger. The prevalence of HTLV-1 infection was 5.1% (CI 95%: 4.2–6.0). In the multiple logistic regression model, HTLV-1 infection was associated with age ≥ 45 years, female sex, being first and second-generation Japanese immigrants, and having sexual partners with history of blood transfusion. The phylogenetic analysis revealed that all HTLV-1 were classified as Cosmopolitan (1a) subtype. Of them, 47.8% were classified as Transcontinental (A) subgroup and 52.2% as belonging to the Japanese (B) subgroup. Although most HTLV-1-infected patients were asymptomatic (97.3%), blurred vision was associated with HTLV-1 infection. The high prevalence of HTLV-1 infection found in this studied population and especially the intra- and interfamily HTLV-1 transmission presents an urgent call for preventive and control responses of this infection in Brazil.
Development of a humanized mouse model to analyze antibodies specific for human leukocyte antigen (HLA)
Authors: Yanagawa Senichiro , Tahara Hiroyuki , Shirouzu Takayuki , Kawai Shintaro , Tanaka Yuka , Ide Kentaro , Akimoto Shuji , Ohdan Hideki
Published: February 2021

In organ transplantation, human leukocyte antigen (HLA)-mismatch grafts not only induce the activation of cellular mediated immune response but also the development of chronic antibody-mediated rejection due to the donor-specific anti-HLA antibody (DSA) produced by B cells and plasma cells interacting with the graft endothelium. Significant improvement in long-term survival after transplantation can be expected if antibody-mediated rejection due to the DSA can be overcome. However, the mechanism of producing or controlling the DSA remains to be elucidated. In recent decades, “humanized” mouse models have been widely used for the basic research of human immune systems, but a humanized mouse model to analyze the mechanism of DSA production has not been established yet. Thus, we aimed to create a humanized mouse using a severe immunodeficiency mouse (NSG mouse) administered with human peripheral blood mononuclear cells (PBMCs). Initially, we detected a very low level of human total-IgG and no anti-HLA antibodies (Abs) in these mice. In our next attempt, we mixed PBMCs of various HLA antigenic combinations with or without regulatory T cells and preconditioned them by culturing on feeder cells stably transfected with human CD40 ligand (h-CD40L) alone or with h-CD40L and human B cell activating factor (h-BAFF). They were subsequently co-cultured with the corresponding irradiated stimulator PBMCs, and all cells were administered into naïve NSG mice. Although all three humanized models had sufficient human total-IgG and anti-HLA antibody production, allospecific anti-HLA Ab production was prominently suppressed whereas non-specific anti-HLA Abs were sufficiently detected. Therefore, this novel humanized mouse model might be useful for analyzing the mechanism of anti-allogeneic human B cell tolerance induction.
Characteristics of HIV seroconverters in the setting of universal test and treat: Results from the SEARCH trial in rural Uganda and Kenya
Authors: Nyabuti Marilyn N. , Petersen Maya L. , Bukusi Elizabeth A. , Kamya Moses R. , Mwangwa Florence , Kabami Jane , Sang Norton , Charlebois Edwin D. , Balzer Laura B. , Schwab Joshua D. , Camlin Carol S. , Black Douglas , Clark Tamara D. , Chamie Gabriel , Havlir Diane V. , Ayieko James
Published: February 2021

Background

Additional progress towards HIV epidemic control requires understanding who remains at risk of HIV infection in the context of high uptake of universal testing and treatment (UTT). We sought to characterize seroconverters and risk factors in the SEARCH UTT trial (NCT01864603), which achieved high uptake of universal HIV testing and ART coverage in 32 communities of adults (≥15 years) in rural Uganda and Kenya.

Methods

In a pooled cohort of 117,114 individuals with baseline HIV negative test results, we described those who seroconverted within 3 years, calculated gender-specific HIV incidence rates, evaluated adjusted risk ratios (aRR) for seroconversion using multivariable targeted maximum likelihood estimation, and assessed potential infection sources based on self-report.

Results

Of 704 seroconverters, 63% were women. Young (15–24 years) men comprised a larger proportion of seroconverters in Western Uganda (18%) than Eastern Uganda (6%) or Kenya (10%). After adjustment for other risk factors, men who were mobile [≥1 month of prior year living outside community] (aRR:1.68; 95%CI:1.09,2.60) or who HIV tested at home vs. health fair (aRR:2.44; 95%CI:1.89,3.23) were more likely to seroconvert. Women who were aged ≤24 years (aRR:1.91; 95%CI:1.27,2.90), mobile (aRR:1.49; 95%CI:1.04,2.11), or reported a prior HIV test (aRR:1.34; 95%CI:1.06,1.70), or alcohol use (aRR:2.07; 95%CI:1.34,3.22) were more likely to seroconvert. Among survey responders (N = 607, 86%), suspected infection source was more likely for women than men to be ≥10 years older (28% versus 8%) or a spouse (51% vs. 31%) and less likely to be transactional sex (10% versus 16%).

Conclusion

In the context of universal testing and treatment, additional strategies tailored to regional variability are needed to address HIV infection risks of young women, alcohol users, mobile populations, and those engaged in transactional sex to further reduce HIV incidence rates.