bluuebunny/crossref_metadata_2025 huggingface dataset:
Lung-Resident Mesenchymal Stem Cell Fates within Lung Cancer
Authors: Sentek Hanna , Klein Diana
Published: September 2021

Lung-resident mesenchymal stem cells (LR-MSCs) are non-hematopoietic multipotent stromal cells that predominately reside adventitial within lung blood vessels. Based on their self-renewal and differentiation properties, LR-MSCs turned out to be important regulators of normal lung homeostasis. LR-MSCs exert beneficial effects mainly by local secretion of various growth factors and cytokines that in turn foster pulmonary regeneration including suppression of inflammation. At the same time, MSCs derived from various tissues of origins represent the first choice of cells for cell-based therapeutic applications in clinical medicine. Particularly for various acute as well as chronic lung diseases, the therapeutic applications of exogenous MSCs were shown to mediate beneficial effects, hereby improving lung function and survival. In contrast, endogenous MSCs of normal lungs seem not to be sufficient for lung tissue protection or repair following a pathological trigger; LR-MSCs could even contribute to initiation and/or progression of lung diseases, particularly lung cancer because of their inherent tropism to migrate towards primary tumors and metastatic sites. However, the role of endogenous LR-MSCs to be multipotent tumor-associated (stromal) precursors remains to be unraveled. Here, we summarize the recent knowledge how ‘cancer-educated’ LR-MSCs impact on lung cancer with a focus on mesenchymal stem cell fates.
Quinacrine Induces Nucleolar Stress in Treatment-Refractory Ovarian Cancer Cell Lines
Authors: Oien Derek B. , Ray Upasana , Pathoulas Christopher L. , Jin Ling , Thirusangu Prabhu , Jung Deokbeom , Kumka Joseph E. , Xiao Yinan , Sarkar Bhattacharya Sayantani , Montoya Dennis , Chien Jeremy , Shridhar Viji
Published: September 2021

A considerable subset of gynecologic cancer patients experience disease recurrence or acquired resistance, which contributes to high mortality rates in ovarian cancer (OC). Our prior studies showed that quinacrine (QC), an antimalarial drug, enhanced chemotherapy sensitivity in treatment-refractory OC cells, including artificially generated chemoresistant and high-grade serous OC cells. In this study, we investigated QC-induced transcriptomic changes to uncover its cytotoxic mechanisms of action. Isogenic pairs of OC cells generated to be chemoresistant and their chemosensitive counterparts were treated with QC followed by RNA-seq analysis. Validation of selected expression results and database comparison analyses indicated the ribosomal biogenesis (RBG) pathway is inhibited by QC. RBG is commonly upregulated in cancer cells and is emerging as a drug target. We found that QC attenuates the in vitro and in vivo expression of nucleostemin (NS/GNL3), a nucleolar RBG and DNA repair protein, and the RPA194 catalytic subunit of Pol I that results in RBG inhibition and nucleolar stress. QC promotes the redistribution of fibrillarin in the form of extranuclear foci and nucleolar caps, an indicator of nucleolar stress conditions. In addition, we found that QC-induced downregulation of NS disrupted homologous recombination repair both by reducing NS protein levels and PARylation resulting in reduced RAD51 recruitment to DNA damage. Our data suggest that QC inhibits RBG and this inhibition promotes DNA damage by directly downregulating the NS–RAD51 interaction. Additionally, QC showed strong synergy with PARP inhibitors in OC cells. Overall, we found that QC downregulates the RBG pathway, induces nucleolar stress, supports the increase of DNA damage, and sensitizes cells to PARP inhibition, which supports new therapeutic stratagems for treatment-refractory OC. Our work offers support for targeting RBG in OC and determines NS to be a novel target for QC.
Clinical and Lifestyle-Related Prognostic Indicators among Esophageal Adenocarcinoma Patients Receiving Treatment at a Comprehensive Cancer Center
Authors: Dighe Shruti G. , Yan Li , Mukherjee Sarbajit , McGillicuddy Cailey S. , Hulme Karen L. , Hochwald Steven N. , Yendamuri Saikrishna , Bain Andrew J. , Robillard Kevin T. , Moysich Kirsten B. , Ambrosone Christine B. , Millen Amy E. , Buas Matthew F.
Published: September 2021

Purpose: The incidence of esophageal adenocarcinoma (EAC) has risen substantially in recent decades, while the average 5-year survival remains only ~20%. Disease stage and treatment are the strongest prognostic factors. The role of lifestyle factors in relation to survival remains uncertain, with a handful of studies to date investigating associations with obesity, smoking, physical activity, diet, or medications. Methods: This study included patients diagnosed with primary adenocarcinoma of the esophagus, gastroesophageal junction, or cardia (N = 371) at Roswell Park Comprehensive Cancer Center between 2003 and 2019. Leveraging extensive data abstracted from electronic medical records, epidemiologic questionnaires, and a tumor registry, we analyzed clinical, behavioral, and environmental exposures and evaluated stage-specific associations with survival. Survival distributions were visualized using Kaplan–Meier curves. Cox proportional hazards regression models adjusted for age, sex, stage, treatment, and comorbidities were used to estimate the association between each exposure and all-cause or cancer-specific mortality. Results: Among patients presenting with localized/regional tumors (stages I–III), current smoking was associated with increased overall mortality risk (HR = 2.5 [1.42–4.53], p = 0.002), while current physical activity was linked to reduced risk (HR = 0.58 [0.35–0.96], p = 0.035). Among patients with stage IV disease, individuals reporting pre-diagnostic use of statins (HR = 0.62 [0.42–0.92], p = 0.018) or NSAIDs (HR = 0.61 [0.42–0.91], p = 0.016) had improved overall survival. Exploratory analyses suggested that high pre-diagnostic dietary consumption of broccoli, carrots, and fiber correlated with prolonged overall survival in patients with localized/regional disease. Conclusion: Our data suggest that lifestyle exposures may be differentially associated with EAC survival based on disease stage. Future investigation of larger, diverse patient cohorts is essential to validate these findings. Our results may help inform the development of lifestyle-based interventions to improve EAC prognosis and quality of life.
Betulinic Acid Protects from Ischemia-Reperfusion Injury in the Mouse Retina
Authors: Musayeva Aytan , Unkrig Johanna C. , Zhutdieva Mayagozel B. , Manicam Caroline , Ruan Yue , Laspas Panagiotis , Chronopoulos Panagiotis , Göbel Marie L. , Pfeiffer Norbert , Brochhausen Christoph , Daiber Andreas , Oelze Matthias , Li Huige , Xia Ning , Gericke Adrian
Published: September 2021

Ischemia/reperfusion (I/R) events are involved in the pathophysiology of numerous ocular diseases. The purpose of this study was to test the hypothesis that betulinic acid protects from I/R injury in the mouse retina. Ocular ischemia was induced in mice by increasing intraocular pressure (IOP) to 110 mm Hg for 45 min, while the fellow eye served as a control. One group of mice received betulinic acid (50 mg/kg/day p.o. once daily) and the other group received the vehicle solution only. Eight days after the I/R event, the animals were killed and the retinal wholemounts and optic nerve cross-sections were prepared and stained with cresyl blue or toluidine blue, respectively, to count cells in the ganglion cell layer (GCL) of the retina and axons in the optic nerve. Retinal arteriole responses were measured in isolated retinas by video microscopy. The levels of reactive oxygen species (ROS) were assessed in retinal cryosections and redox gene expression was determined in isolated retinas by quantitative PCR. I/R markedly reduced cell number in the GCL and axon number in the optic nerve of the vehicle-treated mice. In contrast, only a negligible reduction in cell and axon number was observed following I/R in the betulinic acid-treated mice. Endothelial function was markedly reduced and ROS levels were increased in retinal arterioles of vehicle-exposed eyes following I/R, whereas betulinic acid partially prevented vascular endothelial dysfunction and ROS formation. Moreover, betulinic acid boosted mRNA expression for the antioxidant enzymes SOD3 and HO-1 following I/R. Our data provide evidence that betulinic acid protects from I/R injury in the mouse retina. Improvement of vascular endothelial function and the reduction in ROS levels appear to contribute to the neuroprotective effect.
Characterization and Gene Expression of Vitellogenesis-Related Transcripts in the Hepatopancreas and Ovary of the Red Swamp Crayfish, Procambarus clarkii (Girard, 1852), during Reproductive Cycle
Authors: Manfrin Chiara , Tom Moshe , Avian Massimo , Battistella Silvia , Pallavicini Alberto , Giulianini Piero Giulio
Published: September 2021

The major component of the animal egg yolk is the lipoglycoprotein vitellin, derived from its precursor vitellogenin (VTG), which is produced species-specifically in decapod crustaceans in the hepatopancreas and/or in the ovary of reproductive females. Previous studies on Procambarus clarkii vitellogenesis report the existence of two single VTGs. Here, from a multiple tissue transcriptome including ovaries and hepatopancreas of P. clarkii, we characterized four different VTG and two VTG-like transcriptomes encoding for the discoidal lipoprotein-high density lipoprotein/β-glucan binding protein (dLp/HDL-BGBP). The relative expression of the various genes was evaluated by quantitative Real-Time PCR in both the ovary and hepatopancreas of females at different reproductive stages (from immature until fully mature oocytes). These studies revealed tissue-specificity and a reproductive stage related expression for the VTGs and a constitutive expression in the hepatopancreas of dLp/HDL-BGBP independent from the reproductive stage. This study may lead to more detailed study of the vitellogenins, their transcription regulation, and to the determination of broader patterns of expression present in the female hepatopancreas and ovary during the vitellogenesis. These findings provide a starting point useful for two different practical aims. The first is related to studies on P. clarkii reproduction, since this species is highly appreciated on the market worldwide. The second is related to the study of new potential interference in P. clarkii reproduction to delay or inhibit the worldwide spread of this aggressively invasive species.
Technical Data of Heterologous Expression and Purification of SARS-CoV-2 Proteases Using Escherichia coli System
Authors: Razali Rafida , Subbiah Vijay Kumar , Budiman Cahyo
Published: September 2021

The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the inhibition of viral replication. In order to perform drug discovery assays for SARS-CoV-2, it is imperative that efficient methods are established for the production and purification of 3CLpro and PLpro of SARS-CoV-2, designated as 3CLpro-CoV2 and PLpro-CoV2, respectively. This article expands the data collected in the attempts to express SARS-CoV-2 proteases under different conditions and purify them under single-step chromatography. Data showed that the use of E. coli BL21(DE3) strain was sufficient to express 3CLpro-CoV2 in a fully soluble form. Nevertheless, the single affinity chromatography step was only applicable for 3CLpro-CoV2 expressed at 18 °C, with a yield and purification fold of 92% and 49, respectively. Meanwhile, PLpro-CoV2 was successfully expressed in a fully soluble form in either BL21(DE3) or BL21-CodonPlus(DE3) strains. In contrast, the single affinity chromatography step was only applicable for PLpro-CoV2 expressed using E. coli BL21-CodonPlus(DE3) at 18 or 37 °C, with a yield and purification fold of 86% (18 °C) or 83.36% (37 °C) and 112 (18 °C) or 71 (37 °C), respectively. The findings provide a guide for optimizing the production of SARS-CoV-2 proteases of E. coli host cells.
Coupling Photosynthetic Measurements with Biometric Data to Estimate Gross Primary Productivity (GPP) in Mediterranean Pine Forests of Different Post-Fire Age
Authors: Sazeides Christodoulos I. , Christopoulou Anastasia , Fyllas Nikolaos M.
Published: September 2021

Quantification of forest Gross Primary Productivity (GPP) is important for understanding ecosystem function and designing appropriate carbon mitigation strategies. Coupling forest biometric data with canopy photosynthesis models can provide a means to simulate GPP across different stand ages. In this study we developed a simple framework to integrate biometric and leaf gas-exchange measurements, and to estimate GPP across four Mediterranean pine forests of different post-fire age. We used three different methods to estimate the Leaf Area Index (LAI) of the stands, and monthly gas exchange data to calibrate the photosynthetic light response of the leaves. Upscaling of carbon sequestration at the canopy level was made by implementing a Big Leaf and a Sun/Shade model, using both average and variant (monthly) photosynthetic capacity values. The Big Leaf model simulations systematically underestimated GPP compared to the Sun/Shade model simulations. Our simulations suggest an increasing GPP with age up to a stand maturity stage. The shape of the GPP trend with stand age was not affected by the method used to parameterise the model. At the scale of our study, variability in stand and canopy structure among the study sites seems to be the key determinant of GPP.
New Perspectives for LVL Manufacturing from Wood of Heterogeneous Quality—Part. 1: Veneer Mechanical Grading Based on Online Local Wood Fiber Orientation Measurement
Authors: Duriot Robin , Pot Guillaume , Girardon Stéphane , Roux Benjamin , Marcon Bertrand , Viguier Joffrey , Denaud Louis
Published: September 2021

The grading of wood veneers according to their true mechanical potential is an important issue in the peeling industry. Unlike in the sawmilling industry, this activity does not currently estimate the local properties of production. The potential of the tracheid effect, which enables local fiber orientation measurement, has been widely documented for sawn products. A measuring instrument exploiting this technology and implemented on a peeling line was developed, enabling us to obtain the fiber orientation locally which, together with global density, allowed us to model the local elastic properties of each veneer. A sorting method using this data was developed and is presented here. It was applied to 286 veneers from several logs of French Douglas fir, and was compared to a widely used sorting method based on veneer appearance defects. The effectiveness of both grading approaches was quantified according to mechanical criteria. This study shows that the sorting method used (based on local fiber orientation and average density) allows for better theorical quality discrimination according to the mechanical potential. This article is the first in a series, with the overall aim of enhancing the use of heterogeneous wood veneers in the manufacturing of maximized-performance LVL by veneer grading and optimized positioning as well as material mechanical property modelization.
CNV Detection from Exome Sequencing Data in Routine Diagnostics of Rare Genetic Disorders: Opportunities and Limitations
Authors: Royer-Bertrand Beryl , Cisarova Katarina , Niel-Butschi Florence , Mittaz-Crettol Laureane , Fodstad Heidi , Superti-Furga Andrea
Published: September 2021

To assess the potential of detecting copy number variations (CNVs) directly from exome sequencing (ES) data in diagnostic settings, we developed a CNV-detection pipeline based on ExomeDepth software and applied it to ES data of 450 individuals. Initially, only CNVs affecting genes in the requested diagnostic gene panels were scored and tested against arrayCGH results. Pathogenic CNVs were detected in 18 individuals. Most detected CNVs were larger than 400 kb (11/18), but three individuals had small CNVs impacting one or a few exons only and were thus not detectable by arrayCGH. Conversely, two pathogenic CNVs were initially missed, as they impacted genes not included in the original gene panel analysed, and a third one was missed as it was in a poorly covered region. The overall combined diagnostic rate (SNVs + CNVs) in our cohort was 36%, with wide differences between clinical domains. We conclude that (1) the ES-based CNV pipeline detects efficiently large and small pathogenic CNVs, (2) the detection of CNV relies on uniformity of sequencing and good coverage, and (3) in patients who remain unsolved by the gene panel analysis, CNV analysis should be extended to all captured genes, as diagnostically relevant CNVs may occur everywhere in the genome.
Why Do Medium-Sized Technology Farms Adopt Environmental Innovation? The Mediating Role of Pro-Environmental Behaviors
Authors: Huang Stanley Y. B. , Li Ming-Way , Lee Yue-Shi
Published: September 2021

This research fills several gaps in the literature not investigated in previous studies. First, it examines how the responsible leadership (RL) of the chief executive officer (CEO) influences medium-sized technology farms to adopt environmental innovation (EI) through the pro-environmental behaviors (PBs) of the senior executive team (SET) according to the theory of social learning, as previous research only took institutional theory and utilitarianism as the driving factors of agricultural innovation. Second, we propose the potential growth model (PGM) from a sample of 105 CEOs and their SETs in medium-sized technology farms to handle the problem that an individual may regulate his behaviors based on how he translates and understands the surrounding environment, because previous research has ignored this perspective. Lastly, this research offers recommendations for the implementation of EI in medium-sized technology farms and also expands the related literature on sustainable agricultural production.
Synthesis and Characterization of Super Bulky β-Diketiminato Group 1 Metal Complexes
Authors: Jones Dafydd D. L. , Watts Samuel , Jones Cameron
Published: September 2021

Sterically bulky β-diketiminate (or Nacnac) ligand systems have recently shown the ability to kinetically stabilize highly reactive low-oxidation state main group complexes. Metal halide precursors to such systems can be formed via salt metathesis reactions involving alkali metal complexes of these large ligand frameworks. Herein, we report the synthesis and characterization of lithium and potassium complexes of the super bulky anionic β-diketiminate ligands, known [TCHPNacnac]− and new [TCHP/DipNacnac]− (ArNacnac = [(ArNCMe)2CH]−) (Ar = 2,4,6-tricyclohexylphenyl (TCHP) or 2,6-diisopropylphenyl (Dip)). The reaction of the proteo-ligands, ArNacnacH, with nBuLi give the lithium etherate compounds, [(TCHPNacnac)Li(OEt2)] and [(TCHP/DipNacnac)Li(OEt2)], which were isolated and characterized by multinuclear NMR spectroscopy and X-ray crystallography. The unsolvated potassium salts, [{K(TCHPNacnac)}2] and [{K(TCHP/DipNacnac)}∞], were also synthesized and characterized in solution by NMR spectroscopy. In the solid state, these highly reactive potassium complexes exhibit differing alkali metal coordination modes, depending on the ligand involved. These group 1 complexes have potential as reagents for the transfer of the bulky ligand fragments to metal halides, and for the subsequent stabilization of low-oxidation state metal complexes.
Precise Characterization of Bombyx mori Fibroin Heavy Chain Gene Using Cpf1-Based Enrichment and Oxford Nanopore Technologies
Authors: Lu Wei , Lan Xinhui , Zhang Tong , Sun Hao , Ma Sanyuan , Xia Qingyou
Published: September 2021

To study the evolution of gene function and a species, it is essential to characterize the tandem repetitive sequences distributed across the genome. Cas9-based enrichment combined with nanopore sequencing is an important technique for targeting repetitive sequences. Cpf1 has low molecular weight, low off-target efficiency, and the same editing efficiency as Cas9. There are numerous studies on enrichment sequencing using Cas9 combined with nanopore, while there are only a few studies on the enrichment sequencing of long and highly repetitive genes using Cpf1. We developed Cpf1-based enrichment combined with ONT sequencing (CEO) to characterize the B. mori FibH gene, which is composed of many repeat units with a long and GC-rich sequence up to 17 kb and is not easily amplified by means of a polymerase chain reaction (PCR). CEO has four steps: the dephosphorylation of genomic DNA, the Cpf1 targeted cleavage of FibH, adapter ligation, and ONT sequencing. Using CEO, we determined the fine structure of B. moriFibH, which is 16,845 bp long and includes 12 repetitive domains separated by amorphous regions. Except for the difference of three bases in the intron from the reference gene, the other sequences are identical. Surprisingly, many methylated CG sites were found and distributed unevenly on the FibH repeat unit. The CEO we established is an available means to depict highly repetitive genes, but also a supplement to the enrichment method based on Cas9.
Topical Application of Synthetic Hormones Terminated Reproductive Diapause of a Univoltine Weed Biological Control Agent
Authors: Park Ikju , Smith Lincoln
Published: September 2021

Classical biological control is an important method for controlling invasive alien weeds. Univoltine insects can be highly effective biological control agents of annual weeds because they are well synchronized with their host plant. However, having only one generation per year makes it difficult and slow to multiply them in the laboratory for initial field releases. If it were possible to terminate reproductive diapause early, then we could rear multiple generations per year, which would greatly increase annual production. We used a recently approved biocontrol agent, Ceratapion basicorne (a univoltine weevil), for yellow starthistle (Centaurea solstitialis) as a model system to study the use of two insect hormones, 20-hydroxyecdysone (20E) and methoprene, to terminate reproductive diapause. Methoprene (1 μg applied topically) terminated reproductive diapause of female weevils, whereas doses of 0.0, 0.01 and 0.1 μg did not. The combination of methoprene and 20E had a stronger effect and induced an increase in eggs (1.51 ± 0.16 eggs/day, mean ± SE) compared with a methoprene only group (1.00 ± 0.13 eggs/day), and a control group (0.21 ± 0.04 eggs/day). Thus, topical application of these hormones should enable us to rear the weevil out of its normal season and produce more than one generation per year, which will increase productivity of mass-rearing it for field release. Once released in the field, the insect would continue as a univoltine agent that is well-synchronized with its host plant.
Improving the efficiency and quality of carrying out certification in the agrarian and industrial complex sphere
Authors: Kazakova V. A. , Shinkevich V. A. , Dunayev A. V.
Published: September 2021

One of key problems of improvement of system of standardization is the greatest possible application of the international (regional) standards for implementation (confirmation) of compliance of production, services to the established requirements. In this regard harmonization with the international standards of three interstate standards directed on increase of efficiency and quality of carrying out certification of works (services) in the agrarian and industrial complex sphere is carried out: GOST "Diesels tractor and kombaynovy. Delivery in repair and release from repair. Specifications"; GOST "Tractors agricultural. Delivery in repair and release from repair. Specifications"; GOST "Combines self-propelled grain-harvesting and their assembly units. Delivery in repair and release from repair. Specifications".
Economic efficiency of import-substituting production of spare parts for tractor plows
Authors: Aniskovich G. I. , Kovalev L. I. , Kovalev I. L.
Published: September 2021

The main provisions of the economic substantiation of the production program for the manufacture and hardening of wear parts for imported multi-hull plows of various foreign manufacturers are considered. The production program is developed on the basis of innovative technologies for strengthening the replaceable parts of the working mechanisms of agricultural machinery, created with the cooperation of the Belarusian State Agrarian Technical University with other scientific organizations of Belarus.
Monitoring of the cutting device of shearing machines for sheep
Authors: Rusakov A. N. , Turgenbaev M. S.
Published: September 2021

The article presents monitoring of cutting machines of sheep shearing machines. In general, shearing machines have low-cutting machines, which is due to the production of the largest wool cutting. It was found that with increasing cutting speed, the force on the cut of wool decreases. The speed of submission of the machine at strippers with low qualification is 0,3–0,7 m/s; average 0,4–0,8 m/s; high 0,5–1,1 m/s. The width of the comb grip is the main constructive parameter of the shearing machine, which affects its productivity, priority is given to wide-grasping machines having a width of 76,8 mm, with which the main sheep population is processed.
Robotized complex control system with a modernized electric drive for calibration and transportation of glass tubes for medical and veterinary purposes
Authors: Alekseev V. A. , Kolosov S. P. , Seleznev M. E.
Published: September 2021

The paper considers the process of automating calibration steklomramor for medical and veterinary industry, with the introduction of the functions of vision and of methods of intellectualization of process technology for control of electrical drives for transportation and the management of mechanical parts, with the purpose of increase of efficiency of functioning of the complex.
Modernization of the bench KI-28097M-03 to assess the quality of repair of regulated hydraulic units
Authors: Makarkin I. M. , Dankov A. A. , Ivleva I. B. , Filippova E. M. , Petrishchev N. A.
Published: September 2021

The article presents a prototype of the modernized KI-28097M-03 stand, which provides quality control work for the repair of regulated hydraulic units for modern tractors, self-propelled machines, industrial, agricultural, communal purposes. The use of the stand will allow service enterprises of dealers to minimize the downtime of equipment, improve the quality of repairs, expand the range of services provided to carry out warranty and post-warranty technical support of the equipment being sold.
Unassembled recovery of worn-out interfaces of internal combustion engines with the use of geomodifiers
Authors: Dunaev А. V. , Kostomakhin M. N.
Published: September 2021

In article features of operation of geomodifiers are considered at in-place restoration of worn-out interfaces of internal combustion engine.
Assessment of the durability of self-propelled agricultural machines
Authors: Voronov A. N. , Kostomakhin M. N.
Published: September 2021

Material according to reliability of agricultural machinery and its main indicators which are characterized by measurement of resource parameters of technical condition of details and interfaces is presented in article. The possibility of an assessment of a resource of car/system or the unit is considered, at technical service of cars by means of the generalized coefficient characterizing their current technical condition
ABUNDANCIA POBLACIONAL DE Tetraclita Panamensis (Cirripedia: Tetraclitidae) BAJO DOS DIFERENTES CONDICIONES DE OLEAJE EN ISLA PALMA, PACÍFICO COLOMBIANO
Authors: Lizcano-Sandoval Luis David , Castro Sergio Iván
Published: September 2021

Las poblaciones de balanos como Tetraclita panamensis están sometidos a factores bióticos y abióticos verticales de la zona intermareal, y también son susceptibles a las condiciones horizontales como el oleaje. Este estudio se enfocó en evaluar la abundancia horizontal y distribución vertical de T. panamensis bajo condiciones de oleaje fuerte y calmado, y de temperatura en Isla Palma (Pacifico Colombiano) como una medida indirecta de su adaptación a dichas condiciones. Se realizaron 30 cuadrantes en la zona occidental y 34 cuadrantes en la zona oriental de manera sistemática, en los que se registraron 1239 y 1156 individuos respectivamente. Las poblaciones de ambos lados de la isla no presentaron diferencias significativas (p= 0,319), esto sugieren que las poblaciones de T. panamesis  se encuentran adaptadas a las condiciones de oleaje, mientras que la distribución vertical puede estar influenciada principalmente por las condiciones físicas y biológicas del terreno que reducen los efectos de la temperatura y humedad. Aun faltan estudios que demuestren que el oleaje está afectando el asentamiento y reclutamiento de los balanos en Isla Palma, y también de alguna forma su distribución.
SCIENTIFIC SCHOOLS IN THE INSTITUTE OF PEDAGOGICAL AND ADULT EDUCATION OF THE NATIONAL ACADEMY OF PEDAGOGICAL SCIENCES OF UKRAINE
Authors: Vovk Myroslava , Gryshchenko Yulia
Published: September 2021

The leading trend in the development of Ukrainian and world pedagogical science is fundamentalisation, which is the basis of the "advance" education in the context of sustainable development of society. The primary principles of pedagogy in its interdisciplinary measurement was established through the activities of the university and academic centres where scientific schools were formed in retrospect. Accordance to this, the elaboration of methodological, theoretical, and practice- oriented achievements of scientific schools in the pedagogical and educational environment occurs through the continuity of generations of scientists, the actualization of achievements of experienced scientists, and their combination with the innovations of young scientists. The synthesis of these two layers of science allows solving present issues that arise in modern pedagogical science and educational practice. There is the need to globalise the experience of development of scientific schools in the contemporary education and pedagogical environment of Ukraine, particularly in academic centres, that represents the development of pedagogical science in the personalised dimension and confirms the crystallisation of its fundamental principles and innovative nature of scientific research.We summarised the achievements of the leading academic institution of Ukraine – the Institute of Pedagogical and Adult Education of the National Academy of Pedagogical Sciences of Ukraine – in the personalised context and outlined the prospects for the development of scientific schools of the Institute to meet modern challenges of reforming education and science.We described the trends in the development of scientific schools of the well- known Ukrainian scientists and educators (I. Ziaziun, S. Honcharenko, N. Hychkalo, L. Lukianova, L. Khomych), which were formed on the values of pedagogical mentoring, intellectual leadership, empathy, ethical and aesthetic philosophical guidelines.
MARKETING DIGITAL E ADMINISTRAÇÃO PÚBLICA: AS MÍDIAS SOCIAIS COMO INSTRUMENTO DE PARTICIPAÇÃO SOCIAL E ACCOUNTABILITY
Authors: Barbosa de Carvalho Luciana
Published: September 2021

This study aims to understand the relationship of non-commercial Digital Marketing in Public Administration and the promotion of social participation in public management through social media. Despite the federal legislation establishing mechanisms for the participation of the population, such as consultations and public hearings, social media is gaining more and more space in our lives as a tool for communication, debate and exchange. Marketing 4.0 integrates offline and online, highlighting the importance of user participation during the development process of a particular product or service, which directs us to participatory public management and the development of more efficient and effective public policies . However, even though the use of social media as a tool for social participation and engagement in political discussions is growing, it cannot be confirmed whether this participation actually influences the development of public and social policies. To understand this relationship, a bibliographic review was carried out, complemented with a documentary research with the objective of discussing the theme.
EDUCAÇÃO INCLUSIVA: LEGISLAÇÃO BRASILEIRA E A INCLUSÃO
Authors: Cenci Gasperin Jéssica
Published: September 2021

Today the inclusion of students with disabilities is a reality in Brazilian schools, a right guaranteed by law. guaranteed by law. This article provides insight, through legislation, into the historical construction of inclusion, allowing the elucidation of concepts relevant to the education of people with disabilities. of inclusion, allowing the elucidation of concepts relevant to the education of people with disabilities in the regular school system. the education of people with disabilities in the regular school system. In addition, it allows us to perceive the history of school inclusion in Brazil in parallel with the historical vision of inclusion in the regular school system. in Brazil in parallel with the historical view of disability in the country, as well as to analyze if the legislation guarantees the effective inclusion of students with disabilities in the regular school system. legislation guarantees the effective inclusion of students with disabilities in the regular school system, as well as to regular school system, and also to reflect on the current laws that address inclusive education and their guidelines. The research used a qualitative literature review methodology, based on the legislation on inclusive education, the subject of the project. on inclusive education, the subject of the project. In addition, it was conditioned to the concept and vision of the main authors in the studies of this topic. the main authors of studies on this topic, such as Silva, Ferreira and Mantoan, which were conducted in journals, articles and the Internet. in journals, articles and internet. It is concluded that legislation, in spite of the advances and setbacks throughout history, is indispensable for the throughout history, is indispensable to guarantee and ensure the entry and permanence of these students in regular school, however, it is However, legislation alone does not guarantee the full inclusion of these students, since this depends on the However, legislation alone does not guarantee the full inclusion of these students, as it depends on numerous factors, including the involvement of the management team, teachers, and other and others responsible for pedagogical practice, as well as the elimination of barriers that may impede the full inclusion of these students. that may impede the full inclusion of these individuals in society.
Laboratorios virtuales en entorno de aprendizaje de química orgánica, para el bachillerato ecuatoriano
Authors: Arroba Arroba María Fernanda , Acurio Maldonado Santiago Alejandro
Published: September 2021

La importancia de este estudio sobresale en la implementación de laboratorios virtuales con los estudiantes de tercero de bachillerato de la Unidad Educativa Vicente Anda Aguirre (Ecuador), herramienta que beneficia en la adquisición de saberes que mediante el aprovechamiento de los avances tecnológicos promueve la realización de actividades prácticas, que contribuyen en el refuerzo del conocimiento; lamentablemente, la insuficiente asimilación de los conceptos de química orgánica se refleja en la falta de entusiasmo, y el desinterés para enfrentar retos académicos con autonomía, factor que perjudica en la experiencia del alumno, la participación, experimentación, el análisis y la toma de decisiones; es importante resaltar que por la emergencia sanitaria COVID-19 que afecta a la población, el estudiante no realiza las prácticas en un laboratorio real.
Por las razones expuestas, el objetivo del estudio es implementar estrategias didácticas en entornos virtuales en el proceso de aprendizaje de los contenidos de química orgánica. La indagación se enmarca en un enfoque cuantitativo, la metodología se enfoca en el aprendizaje basado en problemas (ABP), con un alcance correlacional, descriptivo y explicativo. En el proceso de implementación de los laboratorios virtuales, se analiza la capacidad del estudiantado para construir y comprender argumentos, representaciones o modelos que incluyen experimentos llevados a efecto en la virtualidad. Desde este concepto se diseñan estrategias aplicables en el aula de acuerdo a la propuesta curricular y al método de evaluación.
Basic Research o n t he Use of XR Technology t o Support Science Education
Authors:   Faculty of Engineering, Miyazaki University, Miyazaki, Japan., Miyamoto Kodai , Kamasaka Taketo ,   Faculty of Engineering, Miyazaki University, Miyazaki, Japan., Sakamoto Makoto ,   Faculty of Engineering, Miyazaki University, Miyazaki, Japan.
Published: September 2021

As a result of conducting a questionnaire about science classes to high school students in 2016, the percentage of high school students who answered "I like science" and "Science is important" is lower than other subjects. However, more than 80% of elementary and junior high school students said they like experiments and observations. In addition, the 2019 smartphone penetration rate survey found that it is popular among about 90% of students. In addition, VR technology has recently made remarkable progress. From the above, I researched the idea that creating a simulation application using VR technology using smartphones would change the way high school students think about science classes. In this paper, we have developed a simulation application for science experiments. Subjects were asked to experience the newly created app and complete a questionnaire. As a result, the average score is 4 out of 5 and it is not bad. But at the same time, a problem was found. The problem was that this app was a simulation app, so there wasn't much user operability, so I wanted a little more operability. I want to make apps in other fields while improving the problem.
DeepSatData: Building large scale datasets of satellite images for training machine learning models
Authors: Tarasiou Michael
Published: September 2021

This paper presents DeepSatData a pipeline for automatically generating satellite imagery datasets for training machine learning models. We also discuss design considerations with emphasis on dense classification tasks, e.g. semantic segmentation. The implementation presented makes use of freely available Sentinel-2 data which allows the generation of large scale datasets required for training deep neural networks (DNN). We discuss issues faced from the point of view of DNN training and evaluation such as checking the quality of ground truth data and comment on the scalability of the approach.
Zasoby danych ichtiologiczno-rybackich Morskiego Instytutu Rybackiego - Państwowego Instytutu Badawczego
Authors: Całkiewicz Joanna , Wójcik Ireneusz
Published: September 2021

Pierwszą polską placówkę badań morza, Morskie Laboratorium Rybackie (MLR) w Helu powołano 100 lat temu, w czerwcu 1921 roku. Utworzone laboratorium zajmowało się badaniem zasobów ryb w Bałtyku oraz rozpoznawaniem miejsc połowów, mogących służyć polskiemu rybołówstwu. W 1949 roku nastąpiło włączenie Morskiego Laboratorium Rybackiego do Morskiego Instytutu Rybackiego z siedzibą w Gdyni. Od początku istnienia Instytut jest związany z rybołówstwem morskim i jego głównym zadaniem jest prowadzenie prac naukowo-badawczych w tym zakresie. Od 2005 roku monitoring rybacki został znacząco rozwinięty w związku z przystąpieniem Polski do Unii Europejskiej. Polskie rybołówstwo morskie zostało objęte wspólną polityką rybacką UE oraz unijnym programem zbierania danych rybackich. Obecnie operacja gromadzenia danych, zarządzania nimi i ich wykorzystania w sektorze rybołówstwa oraz wspierania doradztwa naukowego w zakresie wspólnej polityki rybołówstwa jest realizowana w ramach Europejskiego Funduszu Morskiego i Rybackiego oraz krajowego Programu Operacyjnego Rybactwo i Morze.
Wybrane kolekcje przyrodnicze Wydziału Biologii i Ochrony Środowiska Uniwersytetu Łódzkiego.
Authors: Kałucka Izabela , Loga Barbara , Michalski Marek , Tończyk Grzegorz , Nadolski Jerzy
Published: September 2021

Przedstawiono wybrane kolekcje przyrodnicze Wydziału Biologii i Ochrony Środowiska Uniwersytetu Łódzkiego. Kolekcja grzybów zdeponowana w Katedrze Algologii i Mykologii, licząca ok. 60000 kopert, należy do największych i najbardziej wartościowych w Polsce. Zawiera grzyby wielkoowocnikowe, grzyby pasożytnicze roślin, porosty (grzyby zlichenizowane), grzyby naporostowe oraz grzybopodobne śluzowce. Pochodzą one w większości z różnych rejonów Polski, a także Europy i Ameryki. Zbiory bezkręgowców Katedry Zoologii Bezkręgowców i Hydrobiologii liczą ponad milion okazów. Są to bezkrę­gowce słodkowodne (głównie owady, pierścienice i mięczaki) oraz zwierzęta morskie (wieloszczety, skorupiaki, szkarłupnie). Kolekcja zawiera materiały z różnych rejonów Polski oraz z Bałkanów, regionu pontokaspijskiego i Północnej Afryki. Zbiory zwierząt morskich pochodzą z regionu Antarktyki oraz Zatoki Biskajskiej. Kolekcje Muzeum Przyrodniczego posiadają nie tylko dużą wartość naukową, ale i historyczną oraz walory ekspozycyjne. Muzeum posiada zbiór paleontologiczny, kolekcję szkieletów, kolekcje ssaków i ptaków oraz zbiór bezkręgowców, zwłaszcza owadów pochodzących z różnych rejonów świata. Łącznie zgromadzono ponad 100000 okazów, w tym eksponaty rzadko spotykane w innych polskich muzeach.
Zasoby informacji o różnorodności morskich Eukaryota w zbiorach naukowych Instytutu Oceanologii Polskiej Akademii Nauk
Authors: Ronowicz Marta , Bałazy Piotr , Chełchowski Maciej , Deja Kajetan , Grzelak Katarzyna , Kędra Monika , Kotwicki Lech , Kwaśniewski Sławomir , Legeżyńska Joanna , Smoła Zofia , Wiktor Józef , Włodarska-Kowalczuk Maria , Kukliński Piotr
Published: September 2021

Instytut Oceanologii Polskiej Akademii Nauk przechowuje 77500 okazów przyrodniczych z obszarów morskich, gromadzonych od 1985 r., czyli od początku istnienia Pracowni Ekosystemów Arktyki, przekształconej później w Zakład Ekologii Morza. Większość zbiorów stanowi zooplankton (około 36000 okazów) zebrany podczas corocznych rejsów badawczych statku s/y Oceania w rejon Mórz Nordyckich. Równie ważne są niewielkie kolekcje gromadzone przez naukowców zajmujących się poszczególnymi grupami systematycznymi. Zasoby Instytutu obejmują okazy: mszywiołów (Bryozoa), skorupiaków (Crustacea) z wyodrębnioną kolekcją krabów pustelników (Paguroidea), a także stułbiopławów (Hydrozoa), wieloszczetów (Polychaeta), mięczaków (Mollusca), sikwiaków (Sipuncula) oraz szkarłupni (Echinodermata), jak również zbiory pierwotniaków (Protista), meiofauny z wyodrębnionymi kolekcjami ryjkogłowów (Kinorhyncha) i nicieni (Nematoda) oraz makrofauny antarktycznej i Zatoki Gdańskiej.W ramach projektu IMBIO (Integracja i mobilizacja danych o różnorodności biotycznej Eukaryota w zasobach polskich instytucji naukowych) posiadane kolekcje okazów zostaną usystematyzowane i uporządkowane, a dane o zasobach zostaną zorganizowane w postaci cyfrowej, w formie baz metadanych. Idea cyfryzacji danych zawartych w opisach kolekcji naukowych i dzielenia się nimi poprzez organizacje: Krajową Sieć Informacji o Bioróżnorodności (KSIB) i Globalną Usługę Informacji o Bioróżnorodności (GBIF) ułatwi szerszy wgląd i korzystanie z dotychczas niedostępnych zbiorów naukowych.
Obserwacje ornitologiczne w zbiorach Stacji Badania Wędrówek Ptaków Uniwersytetu Gdańskiego
Authors: Nowakowski Jarosław , Stępniewski Krzysztof
Published: September 2021

Wydziału Biologii Uniwersytetu Gdańskiego, jest w posiadaniu jednego z największych i najbardziej wszechstronnych na świecie zbiorów danych ornitologicznych. W tym przypadku zbiór nie obejmuje okazów, tylko notatki z obserwacji lub zdjęcia, głównie dane zaobrączkowania i ponownego stwierdzenia ptaków, ich biometrię oraz wyniki dodatkowych badań (m.in. testy orientacyjne, karty pierzeń oraz kolekcja fotografii skrzydeł). Łącznie zbiór danych dotyczących obrączkowanych ptaków, który jest w posiadaniu Stacji, zawiera około 2200000 rekordów z niemal 600 gatunków ptaków z Europy, Bliskiego Wschodu, Afryki i Ameryki Północnej. Na szczególną uwagę zasługuje zbiór danych biometrycznych, który jest tworzony według niezmienianego od 1964 r. standardu metodycznego, co czyni go najdłuższą i najwszechstronniejszą serią danych tego typu na świecie. Również zbiór 70000 wyników testów kierunkowych przeprowadzonych na ok. 100 gatunkach ptaków w Europie, Azji i Afryce jest absolutnie wyjątkowy w skali światowej. W artykule zaprezentowano szeroki wachlarz analiz i przykłady publikacji, które powstały na bazie danych zdeponowanych w archiwach SBWP.
Szymon Tenenbaum (1892-1941) - życiorys spisany na entomologicznej etykiecie
Authors: Iwan Dariusz , Daszkiewicz Piotr
Published: September 2021

Szymon Tenenbaum urodził się 31 stycznia 1892 w Warszawie w rodzinie pochodzenia żydowskiego. Jego żona, Eleonora, w 1947 roku przekazała Państwowemu Muzeum Zoologicznemu zarówno księgozbiór, jak i kolekcję chrząszczy, które stanowiły własność rodziny. Rodziny, która doświadczyła tragedii holokaustu, przeżyła tylko żona słynnego entomologa. Tenenbaum związany był z PMZ w okresie 20-lecia międzywojennego, nie tylko jako członek korespondent, ale również naukowiec, który ratował i porządkował zbiory entomologiczne po pożarze w 1935 roku a także autor kolekcji zoologicznej, o wyjątkowym znaczeniu, wybiegającym poza wartość naukową zwykłych kolekcji owadów. Tenenbaum zmarł w getcie warszawskim 29 listopada 1941 roku, jednak jego zbiór chrząszczy przetrwał II wojnę światową chroniony w Ogrodzie Zoologicznym w Warszawie w Willi pod zwariowana gwiazdą. Dom ten odegrał istotną rolę przy wyprowadzeniu z getta i uratowaniu wielu osób przez Jana i Antoninę Żabińskich. W czasie Powstania Warszawskiego kolekcja Tenenbauma przechowywana była w piwnicy budynku PMZ przy ulicy Wilczej 64 w Warszawie. Zbiór ponad 250000 etykiet muzealnych dołączonych do okazów chrząszczy stanowi olbrzymi zasób danych umożliwiających odtworzenie miejsc i dat pobytu wielkiego entomologa, a także ustalenie jego współpracowników. W kolekcji Tenenbauma, jako nabytki, znajdują się również okazy pozyskiwane w czasach Gabinetu Zoologicznego przez najwybitniejszych polskich przyrodników takich jak Antoni Waga, Władysław Taczanowski, Ksawery i Aleksander Braniccy, Benedykt Dybowski, Konstanty Jelski, Ludwik Hildt. Okazy te są jednymi z nielicznych jakie zachowały się w polskich kolekcjach. W 1944 roku, po upadku Powstania Warszawskiego, Niemcy celowo podpalili budynek Muzeum i zniszczyli najcenniejsze polskie zbiory entomologiczne. Straty materialne i osobowe PMZ doskonale oddają ogólny obraz zniszczeń nauki polskiej w czasie II wojny światowej, których skutki odczuwalne są do dnia dzisiejszego.
Zbiory naukowe Wydziału Biologii i Biotechnologii Uniwersytetu Marii Curie-Skłodowskiej w Lublinie
Authors: Czarnecka Joanna , Mułenko Wiesław , Staniec Bernard
Published: September 2021

Praca prezentuje historię powstania oraz zasoby cennych naukowych kolekcji botaniczno-mykologicznych i entomologicznych zgromadzonych na Wydziale Biologii i Biotechnologii Uniwersytetu Marii Curie-Skłodowskiej w Lublinie. Najstarsze z nich pochodzą z połowy XIX wieku. Dodatkowo przedstawiono wkład niektórych autorów w tworzenie zbiorów i ich powiększanie, a także możliwości wykorzystania tych kolekcji w nauce.