bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Recorded Response of a School Building Heavily Damaged by the 2016 Central Italy Earthquake
Authors: De Sortis Adriano , Vestroni Fabrizio , Marchesini Sara , Nicoletti Mario
Published: June 2022

The OSS (acronym of the Italian “Osservatorio Sismico delle Strutture”) is a network of permanent seismic monitoring systems installed on about 150 buildings in Italy. Each system consists of a number of sensors sufficient to completely describe the dynamic response of the structure. Structural typologies have been selected according to their representativeness of the public building stock and to their importance for emergency management. Data available for each building include: structural survey and in situ tests on materials, experimental modal analysis, finite element models and model updating. In the above framework, a school building located in Amatrice, a small town in the meizoseismal area heavily impacted by the 2016 Central Italy earthquake, is one of the buildings instrumented by the OSS; thus, its monitored dynamic response, including that under the main shock, is available. The building showed diffused huge damage with partial collapses. Firstly, a linear finite element model is used in the interpretation of small amplitude vibrations in order to give a reliable dynamic characterization of the initial conditions of the structure. Some relevant quantities of the recorded structural motion under seismic excitation are used to describe the experimental dynamic behaviour. The recorded displacements are then applied to a nonlinear finite element model, and the numerical results are compared with the experimental evidence. The main aim is to discuss the capability of the combined use of experimental response and computational tools in quantifying damage suffered by a structure on the basis of the measured response to an earthquake.
Does the New FIGO 2018 Staging System Allow Better Prognostic Differentiation in Early Stage Cervical Cancer? A Dutch Nationwide Cohort Study
Authors: Ten Eikelder Mieke L. G. , Hinten Floor , Smits Anke , Van der Aa Maaike A. , Bekkers Ruud L. M. , IntHout Joanna , Wenzel Hans H. B. , Zusterzeel Petra L. M.
Published: June 2022

The FIGO 2018 staging system was introduced to allow better prognostic differentiation in cervical cancer, causing considerable stage migration and affecting treatment options. We evaluated the accuracy of the FIGO 2018 staging in predicting recurrence free (RFS) and overall survival (OS) compared to FIGO 2009 staging in clinically early stage cervical cancer. We conducted a nationwide retrospective cohort study, including 2264 patients with preoperative FIGO (2009) IA1, IA2 and IB1 cervical cancer between 2007–2017. Kaplan–Meier analyses were used to assess survival outcomes. Logistic regression was used to assess risk factors for lymph node metastasis and parametrial invasion. Stage migration occurred in 48% (22% down-staged, 26% up-staged). Survival data of patients down-staged from IB to IA1/2 disease were comparable with FIGO 2009 IA1/2 and better than patients remaining stage IB1. LVSI, invasion depth and parametrial invasion were risk factors for lymph node metastases. LVSI, grade and age were associated with parametrial invasion. In conclusion, the FIGO 2018 staging system accurately reflects prognosis in early stage cervical cancer and is therefore more suitable than the FIGO 2009 staging. However subdivision in IA1 or IA2 based on presence or absence of LVSI instead of depth of invasion would have improved accuracy. For patients down-staged to IA1/2, less radical surgery seems appropriate, although LVSI and histology should be considered when determining the treatment plan.
Genetic Disruption of the γ-Glutamylcysteine Ligase in PDAC Cells Induces Ferroptosis-Independent Cell Death In Vitro without Affecting In Vivo Tumor Growth
Authors: Daher Boutaina , Meira Willian , Durivault Jerome , Gotorbe Celia , Pouyssegur Jacques , Vucetic Milica
Published: June 2022

The conceptualization of a novel type of cell death, called ferroptosis, opens new avenues for the development of more efficient anti-cancer therapeutics. In this context, a full understanding of the ferroptotic pathways, the players involved, their precise role, and dispensability is prerequisite. Here, we focused on the importance of glutathione (GSH) for ferroptosis prevention in pancreatic ductal adenocarcinoma (PDAC) cells. We genetically deleted a unique, rate-limiting enzyme for GSH biosynthesis, γ-glutamylcysteine ligase (GCL), which plays a key role in tumor cell proliferation and survival. Surprisingly, although glutathione peroxidase 4 (GPx4) has been described as a guardian of ferroptosis, depletion of its substrate (GSH) led preferentially to apoptotic cell death, while classical ferroptotic markers (lipid hydroperoxides) have not been observed. Furthermore, the sensitivity of PDAC cells to the pharmacological/genetic inhibition of GPx4 revealed GSH dispensability in this context. To the best of our knowledge, this is the first time that the complete dissection of the xCT-GSH-GPx4 axis in PDAC cells has been investigated in great detail. Collectively, our results revealed the necessary role of GSH in the overall redox homeostasis of PDAC cells, as well as the dispensability of this redox-active molecule for a specific, antioxidant branch dedicated to ferroptosis prevention.
Photocatalytic Remediation of Harmful Alexandrium minutum Bloom Using Hybrid Chitosan-Modified TiO2 Films in Seawater: A Lab-Based Study
Authors: Ibrahim Nur Hanisah , Iqbal Anwar , Mohammad-Noor Normawaty , Razali Roziawati Mohd , Sreekantan Srimala , Yanto Dede Heri Yuli , Mahadi Abdul Hanif , Wilson Lee D.
Published: June 2022

The uncontrolled growth of harmful algal blooms (HABs) can negatively impact the environment and pose threats to human health and aquatic ecosystems. Titanium dioxide (TiO2) is known to be effective in killing harmful algae through flocculation and sedimentation. However, TiO2 in a dispersed form can harm other non-target marine organisms, which has raised concerns by environmentalists and scientists. This research seeks to explore the utility of immobilized titanium oxide as a photocatalyst for mitigation of HABs, where the Alexandrium minutum bloom was used as a model system herein. Chitosan was modified with 0.2 wt.% TiO2 (Chi/TiO2 (x mL; x = 1, 3 and 5 mL) and the corresponding films were prepared via solvent casting method. Scanning electron microscope (SEM) images of the films reveal a highly uneven surface. X-ray diffraction (XRD) analysis indicates the reduction in chitosan crystallinity, where the presence of TiO2 was negligible, in accordance with its dispersion within the chitosan matrix. The photocatalytic mitigation of A.minutum was carried out via a physical approach in a laboratory-scale setting. The negative surface charge of the films was observed to repel the negatively charged A.minutum causing fluctuation in the removal efficiency (RE). The highest RE (76.1 ± 13.8%) was obtained when Chi/TiO2 (1 mL) was used at 72 h, where the hydroxyl radicals generated were inferred to contribute to the deactivation of the algae cells by causing oxidative stress. An outcome of this study indicates that such hybrid films have the potential to replace the non-immobilized (dispersed) TiO2 for HAB mitigation. However, further investigation is required to deploy these films for field applications at a larger scale.
Assessment of Climate Models Performance and Associated Uncertainties in Rainfall Projection from CORDEX over the Eastern Nile Basin, Ethiopia
Authors: Yimer Sadame M. , Bouanani Abderrazak , Kumar Navneet , Tischbein Bernhard , Borgemeister Christian
Published: June 2022

The adverse impact of climate change on different regionally important sectors such as agriculture and hydropower is a serious concern and is currently at the epicentre of global interest. Despite the extensive efforts to project the future climate and assess its potential impact, it is surrounded by uncertainties. This study aimed to assess climate models’ performance and associated uncertainties in rainfall projection over the eastern Nile basin, Ethiopia. Seventeen climate models from Coordinated Regional Climate Downscaling Experiment (CORDEX) and their four ensemble models were evaluated in terms of their historical prediction performance (1986–2005) and future simulation skill (2006–2016) at rainfall station (point location), grid-scale (0.44° × 0.44°) and basin scale. Station-based and spatially interpolated observed rainfall data were used as a reference during climate model performance evaluation. In addition, CRU data was used as an alternative reference data to check the effect of the reference data source on the climate models evaluation process. As the results showed, climate models have a large discrepancy in their projected rainfall and hence prior evaluation of their performance is necessary. For instance, the bias in historical mean annual rainfall averaged over the basin ranges from +760 mm (wet bias) to −582 mm (dry bias). The spatial pattern correlation (r) of climate models output and observed rainfall ranges from −0.1 to 0.7. The ensemble formed with selected (performance-based) member models outperforms the widely used multi-model ensemble in most of the evaluation metrics. This showed the need for reconsidering the widely used multi-model approach in most climate model-based studies. The use of CRU data as a reference resulted in a change in the magnitude of climate model bias. To conclude, each climate model has a certain degree of uncertainty in the rainfall projection, which potentially affects the studies on climate change and its impact (e.g., on water resources). Therefore, climate-related studies have to consider uncertainties in climate projections, which will help end-users (decision-makers) at least to be aware of the potential range of deviation in the future projected outcomes of interest.
The Fabrication of Oleophobic Coating and Its Application in Particulates Filtration
Authors: Zhang Yuqiong , Qing Chenglin , Lin Yixuan , Guan Yunlong , Dai Wenhua , Yang Yingxia , Deng Gaofeng , Guan Li
Published: June 2022

The stir-frying process in Chinese cooking has produced serious emissions of oily particles, which are an important source of urban air pollution. In particular, the complex composition of fine particulate may pose a threat to human respiratory and immune systems. However, current filtration methods for oily particulate fumes have low filtration efficiency, high resistance, and high equipment costs. In polypropylene (PP) electret filters, efficiency rapidly decreases and pressure drop (wind resistance) sharply increases after the adsorption of oily particles, due to the oleophilic properties of the PP fibre. We addressed this issue of filter performance degradation by fabricating a sodium perfluorooctanoate (SPFO) oleophobic coating on polyvinylidene fluoride (PVDF) fibre membranes for oily particle filtration. The SPFO coating showed a promising oleophobic effect even at low concentrations, which suggests it has oleophobic properties for different oil types and can be modified for different substrates. This fabricated oleophobic coating is thermostable and the oleophobic effect is unaffected by temperatures from 0 to 100 °C. By modifying the SPFO coating on the PVDF membrane, a high filtration efficiency (89.43%) and low wind resistance (69 Pa) was achieved without oil adhesion, so the proposed coating can be applied in the filtration and purification of oily fine particles and offers a potential strategy for preventing atmospheric oil pollution.
Pickering Emulsions: A Novel Tool for Cosmetic Formulators
Authors: Guzmán Eduardo , Ortega Francisco , Rubio Ramón G.
Published: June 2022

The manufacturing of stable emulsion is a very important challenge for the cosmetic industry, which has motivated intense research activity for replacing conventional molecular stabilizers with colloidal particles. These allow minimizing the hazards and risks associated with the use of conventional molecular stabilizers, providing enhanced stability to the obtained dispersions. Therefore, particle-stabilized emulsions (Pickering emulsions) present many advantages with respect to conventional ones, and hence, their commercialization may open new avenues for cosmetic formulators. This makes further efforts to optimize the fabrication procedures of Pickering emulsions, as well as the development of their applicability in the fabrication of different cosmetic formulations, necessary. This review tries to provide an updated perspective that can help the cosmetic industry in the exploitation of Pickering emulsions as a tool for designing new cosmetic products, especially creams for topical applications.
Chemical and Microstructural Properties of Fly Ash and Fly Ash/Slag Activated by Waste Glass-Derived Sodium Silicate
Authors: Bondar Dali , Vinai Raffaele
Published: June 2022

Sodium silicate is commonly used for activating alumina silicates to produce alkali-activated binders that can compete with conventional Portland cement in concrete. However, the cost and emissions related to activators can hinder the use of alkali-activated materials in the industry. The novel, waste-based activators have been developed in the last years, using Si-rich waste streams. Processing waste glass cullet not only reduces the glass landfill disposal but also allows the production of sodium silicate for alkali activation. In this article, the chemical and microstructural properties of neat fly ash and blended 60 fly ash/40 slag pastes activated by sodium silicate produced from glass cullet were studied and compared to equivalent ones activated by commercially available sodium silicate and sodium hydroxide solutions. Fourier transform infrared (FTIR) spectroscopy, X-ray powder diffraction (XRD), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) were used to determine the microstructure and composition of the gel phase. Findings have confirmed that pastes activated by the processed waste glass showed chemical and microstructural properties comparable to pastes produced with commercially available activators.
A Fast Recovery Vertical Superjunction MOSFET with n-Si and p-3C-SiC Pillars
Authors: Gao Rongyu , Cheng Hongyu , Li Wenmao , Deng Chenkai , Chen Jianguo , Wang Qing , Yu Hongyu
Published: June 2022

In the traditional SJ MOSFET structure, n/p pillars with the same doping concentrations in the drift region are introduced to decrease the on-resistance. However, SJ MOSFET will turn on the parasitic diodes due to fast reverse recovery, further inducing severe oscillation in the reverse recovery of the device and the corresponding adverse effect on the circuit. In this study, a fast recovery vertical superjunction (SJ) MOSFET with n-Si and p-3C-SiC pillars was studied. Unlike other structures, such as the 4H-SiC superjunction UMOSFET with a heterojunction diode or the ultra-low recovery charge cell-distributed Schottky contacts SJ-MOSFET with integrated isolated NMOS, we introduce a Schottky barrier diode (SBD) on the source contact at the top of the n-Si pillar in the SJ-MOSFET to improve the device reverse recovery. The simulation software TCAD Silvaco was utilized to simulate the device properties. Compared with the conventional Si SJ, the proposed Si/SiC SJ with the Schottky barrier diode (SBD) connected demonstrated a lower reverse recovery charge, which was reduced by 90.5%, respectively. The waveform of the reverse recovery current demonstrates that the electrons in the device are withdrawn from SBD during reverse recovery, preventing the opening of the parasitic diode in the SJ MOSFET. Finally, another structure is illustrated to decrease the gate capacitance by introducing a thin p-base layer between the gate metal and N-Si pillar so that it can improve the switching characteristics of devices. The open-loss and off-loss of the improved device were reduced by 33% and 42.3%, respectively.
Pharmacogenomics of Methotrexate Pathway in Rheumatoid Arthritis Patients: Approach toward Personalized Medicine
Authors: Abdallah Hoda Y. , Ibrahim Maha E. , Abd El-Fadeal Noha M. , Ali Dina A. , Elsehrawy Gehad G. , Badr Rasha E. , Hassoba Howayda M.
Published: June 2022

Background: Methotrexate (MTX) is one of the most common medications used for rheumatoid arthritis (RA) treatment. Single-nucleotide polymorphisms (SNPs) could potentially predict variability in therapeutic outcomes. Aim: This study aims to assess the impact of SNPs in genes encoding for the MTX pathway for predicting clinical and therapeutic responses to MTX in a cohort of Egyptian patients with RA. Subjects and Methods: Data from 107 Egyptian RA patients (aged 44.4 ± 11.4 years) treated with MTX monotherapy, for a duration of 3.7 ± 3.3 years, were collected. Genotypes of 10 SNPs from four different genes were analyzed using the allelic discrimination PCR technique. Results: The ATIC rs3821353 G/T (p = 0.034) and the C/T and C/C of SLC19A1 rs7279445 (p = 0.0018) were associated with a non-response to MTX, while DHFR rs10072026 C/T and C/C were associated with a good response (p < 0.001). Carriers of the ATIC rs382135 3 G (p = 0.001) and ATIC rs4673990 G (p < 0.001) alleles were more likely to develop RA, while the SLC19A1 rs11702425 T (p < 0.001) and GGH rs12681874 T (p = 0.003) allele carriers were more likely to be protected against RA. Carriers of the ATIC rs4673990 A/G genotype (p < 0.001) were at risk of developing RA, while carriers of the following genotypes were mostly protected against RA: ATIC rs3821353 T/T (p < 0.001), ATIC rs3821353 G/G (p = 0.004), SLC19A1 rs11702425 T/T (p = 0.001), SLC19A1 rs11702425 C/T (p = 0.003), GGH rs12681874 C/T (p = 0.004) and GGH rs12681874 T/T (0.002). Conclusion: The genotyping of genes involved in the MTX pathway may be helpful to predict which RA patients will/will not benefit from MTX, and thus, may help to apply a personalized medicine approach in RA.
BI-RADS-Based Classification of Mammographic Soft Tissue Opacities Using a Deep Convolutional Neural Network
Authors: Sabani Albin , Landsmann Anna , Hejduk Patryk , Schmidt Cynthia , Marcon Magda , Borkowski Karol , Rossi Cristina , Ciritsis Alexander , Boss Andreas
Published: June 2022

The aim of this study was to investigate the potential of a machine learning algorithm to classify breast cancer solely by the presence of soft tissue opacities in mammograms, independent of other morphological features, using a deep convolutional neural network (dCNN). Soft tissue opacities were classified based on their radiological appearance using the ACR BI-RADS atlas. We included 1744 mammograms from 438 patients to create 7242 icons by manual labeling. The icons were sorted into three categories: “no opacities” (BI-RADS 1), “probably benign opacities” (BI-RADS 2/3) and “suspicious opacities” (BI-RADS 4/5). A dCNN was trained (70% of data), validated (20%) and finally tested (10%). A sliding window approach was applied to create colored probability maps for visual impression. Diagnostic performance of the dCNN was compared to human readout by experienced radiologists on a “real-world” dataset. The accuracies of the models on the test dataset ranged between 73.8% and 89.8%. Compared to human readout, our dCNN achieved a higher specificity (100%, 95% CI: 85.4–100%; reader 1: 86.2%, 95% CI: 67.4–95.5%; reader 2: 79.3%, 95% CI: 59.7–91.3%), and the sensitivity (84.0%, 95% CI: 63.9–95.5%) was lower than that of human readers (reader 1:88.0%, 95% CI: 67.4–95.4%; reader 2:88.0%, 95% CI: 67.7–96.8%). In conclusion, a dCNN can be used for the automatic detection as well as the standardized and observer-independent classification of soft tissue opacities in mammograms independent of the presence of microcalcifications. Human decision making in accordance with the BI-RADS classification can be mimicked by artificial intelligence.
The Diversity of Melia azedarach L. from China Based on Transcriptome-Developed SSR Marker
Authors: Cai Jinfeng , Yang Xiaoming , Yu Wanwen , Xiang Peng , Zhang Shuqing , Wang Guibin
Published: June 2022

Melia azedarach L. is a native tree species that can be used in a comprehensive way and is widely distributed in all provinces south of the Yellow River in China. Genetic diversity analysis of different M. azedarach germplasm sources is an important basic work for the selection, evaluation, and genetic improvement of M. azedarach germplasm resources. In this study, 100 pairs of SSR primers were designed and synthesized based on M. azedarach transcriptome data, and 16 pairs of reliable SSR primers were finally selected. The developed primers were used to analyze the genetic diversity of M. azedarach from 15 sources in 10 provinces in East, Central, and South China. The results showed that the frequency of the M. azedarach transcriptome SSR loci was high, and the distribution density was high. There were 15 sources of M. azedarach genetic diversity at a moderate level, and genetic variation was mainly present within the sources. The present study further enriches the existing SSR marker database of the M. azedarach family and can provide a reference for genetic diversity analysis and molecularly assisted breeding of M. azedarach plants at the genomic level.
Hydrogen and Methane Production from Anaerobic Co-Digestion of Sorghum and Cow Manure: Effect of pH and Hydraulic Retention Time
Authors: Dareioti Margarita A. , Tsigkou Konstantina , Vavouraki Aikaterini I. , Kornaros Michael
Published: June 2022

The need for alternative energy sources is constantly growing worldwide, while the focus has shifted to the valorization of biomass. The aim of the present study was to determine the optimal pH and hydraulic retention time (HRT) values for treating a mixture of sorghum biomass solution with liquid cow manure (in a ratio 95:5 v/v) through anaerobic digestion, in a two-stage system. Batch tests were initially carried out for the investigation of the pH effect on bio-hydrogen and volatile fatty acids (VFA) production. The highest hydrogen yield of 0.92 mol H2/mol carbohydratesconsumed was obtained at pH 5.0, whereas the maximum degradation of carbohydrates and VFA productivity was observed at pH 6.0. Further investigation of the effect of HRT on hydrogen and methane production was carried out. The maximum yield of 1.68 mol H2/mol carbohydratesconsumed was observed at an HRT of 5 d, with H2 productivity of 0.13 L/LR·d. On the other hand, the highest CH4 production rate of 0.44 L/LR·d was achieved at an HRT of 25 d, with a methane yield of 295.3 mL/g VSadded, whereas at a reduced HRT of 20 d the process exhibited inhibition and/or overload, as indicated by an accumulation of VFAs and decline in CH4 productivity.
An Experimental Study of the Aeroacoustic Properties of a Propeller in Energy Harvesting Configuration
Authors: Candeloro Paolo , Martellini Edoardo , Nederlof Robert , Sinnige Tomas , Pagliaroli Tiziano
Published: June 2022

The aim of the present manuscript is to investigate the noise footprint of an isolated propeller in different flight configurations for the propulsion of a hybrid-electric aircraft. Experimental tests were performed at the Low-Turbulence Tunnel located at Delft University of Technology with a powered propeller model and flush-mounted microphones in the tunnel floor. The propeller was investigated at different advance ratios in order to study the noise impact in propulsive and energy harvesting configurations. For brevity, this work only reports the results at the conditions of maximum efficiency in both propulsive and energy harvesting regimes, for a fixed blade pitch setting. Comparing these two configurations, a frequency-domain analysis reveals a significant modification in the nature of the noise source. In the propulsive configuration, most of the energy is related to the tonal noise component, as expected for an isolated propeller; however, in energy harvesting configuration, the broadband noise component increases significantly compared to the propulsive mode. A more detailed analysis requires separation of the two noise components and, for this purpose, an innovative decomposition strategy based on proper orthogonal decomposition (POD) has been defined. This novel technique shows promising results; both in the time and in the Fourier domains the two reconstructed components perfectly describe the original signal and no phase delays or other mathematical artifices are introduced. In this sense, it can represent a very powerful tool to identify noise sources and, at the same time, to define a proper control strategy aimed at noise mitigation.
A Review of the Phytochemistry and Bioactivity of Clover Honeys (Trifolium spp.)
Authors: Sultana Sharmin , Foster Kevin , Lim Lee Yong , Hammer Katherine , Locher Cornelia
Published: June 2022

This review covers a comprehensive overview of the phytoconstituents and bioactivities reported to date for clover honeys produced from various Trifolium spp. against the backdrop of a more general discussion of the chemistry and bioactivity of these important agricultural species. While research into the phytochemical composition of various honeys and their associated bioactivities is growing, this review demonstrates that the literature to date has seen only a limited number of studies on clover honeys. Surprisingly, there appear to be no comparative data on the concentration of flavonoids in general or isoflavonoids specifically in different clover honeys, although the latter have been identified as a main group of bioactive compounds in red clover plants. Based on the findings of this review, the presence of phytoestrogenic isoflavonoids (e.g., formononetin, biochanin A, genistein, daidzein, glycitein) in clover plants and, by extension, in clover honeys should be further investigated, specifically of clover species outside the three popular perennial clovers (red, white and alsike clovers) to exploit new opportunities of potential benefit to both the pharmaceutical and apiculture industries.
In Vitro Digestive Analysis of Digestible and Resistant Starch Fractions, with Concurrent Glycemic Index Determination, in Whole Grain Wheat Products Minimally Processed for Reduced Glycaemic Impact
Authors: Monro John , Mishra Suman
Published: June 2022

Eight wheat products differing in texture (porridge vs. bread), grain fineness (fine, kibbled, intact), and cooking (raw vs. cooked), with pre-measured glycaemic indexes (GI), were analysed by in vitro amylolytic digestion to determine effects of processing to reduce GI on quantities of starch fractions differing in digestibility. The accuracy and precision of the in vitro analysis was assessed from its ability to concurrently predict clinical GI. In porridges, kernel intactness and lack of cooking reduced GI while increasing Type 1 (inaccessible) and Type 2 (ungelatinised) resistant starch. Porridge in vitro GI values (GIiv), calculated from the area under in vitro digestion curves minus estimated blood glucose disposal, were: raw fine, 26.3; raw kibbled, 12.6; cooked fine, 63.9; cooked kibbled, 44.1; and correlated closely with clinical GI values (R2 = 0.97). In bread, the negative association of kernel intactness and resistant starch with GI was seen in vitro but not in vivo. Bread GIiv values were: roller milled flour, 67.4; stoneground flour 61.1; kibbled grain, 53.0; kibbled + intact kernel, 49.5; but correlation with clinical values was low (R2 = 0.47), and variability in the clinical results was high (clinical CV = 72.5%, in vitro CV = 3.7%). Low glycaemic potency of wheat by minimal processing was achieved by maintaining particle size, avoiding hydrothermal treatment, avoiding crushing and using a food matrix requiring little chewing for ingestion. Use of in vitro digestive analysis for high precision measurement of starch fractions with potential secondary health benefits was validated by accurate concurrent prediction of the glycaemic index but needed to account for effects of chewing.
Potential Application of Living Microorganisms in the Detoxification of Heavy Metals
Authors: Chen Runqiu , Tu Huaijun , Chen Tingtao
Published: June 2022

Heavy metal (HM) exposure remains a global occupational and environmental problem that creates a hazard to general health. Even low-level exposure to toxic metals contributes to the pathogenesis of various metabolic and immunological diseases, whereas, in this process, the gut microbiota serves as a major target and mediator of HM bioavailability and toxicity. Specifically, a picture is emerging from recent investigations identifying specific probiotic species to counteract the noxious effect of HM within the intestinal tract via a series of HM-resistant mechanisms. More encouragingly, aided by genetic engineering techniques, novel HM-bioremediation strategies using recombinant microorganisms have been fruitful and may provide access to promising biological medicines for HM poisoning. In this review, we summarized the pivotal mutualistic relationship between HM exposure and the gut microbiota, the probiotic-based protective strategies against HM-induced gut dysbiosis, with reference to recent advancements in developing engineered microorganisms for medically alleviating HM toxicity.
Occurrence of Patulin and Polyphenol Profile of Croatian Traditional and Conventional Apple Cultivars during Storage
Authors: Skoko Ana-Marija Gotal , Vilić Ružica , Kovač Marija , Nevistić Ante , Šarkanj Bojan , Lores Marta , Celeiro Maria , Babojelić Martina Skendrović , Kovač Tihomir , Lončarić Ante
Published: June 2022

Apples and apple-based products are among the most consumed fruits around the world. However, they are susceptible to infection with the fungi Penicilium expansum. In addition to the reduction of apple quality, secondary metabolism of this fungus produces a mycotoxin patulin that has a negative effect on human health. Currently, there is no available research in the literature on the resistance of Croatian traditional apple cultivars to contamination with P. expansum, and consequently, on the patulin content in apples and apple juice produced from those apples. Although the mechanism of apple resistance to fungal diseases has not yet been sufficiently investigated, some studies have shown that polyphenolic compounds have some impact on fungi growth. In order to contribute with new knowledge, this research deals with monitoring the growth of P. expansum on apples, patulin detection by LC/MS-MS, determination of polyphenol profile by validated HPLC method, and determining the effect of polyphenolic compounds on fungi growth and patulin production during apple storage. The results of this study have shown that Croatian traditional apple cultivars harvested from family farm Horvatić contain higher concentration of polyphenolic compounds and higher antioxidant activity. At the same time, they showed more resistance to infection by P. expansum than conventional ones. The higher content of dihydrochalcones and flavanols encouraged the biosynthesis of patulin in examined cultivars. However, the higher content of non-flavonoids such as 2-6 dimethoxybenzoic acid, 4-hydroxycinnamic acid and chlorogenic acid leads to decrease in content of patulin. In conclusion, it seems that content of polyphenols and patulin production are correlated.
Prevalence and Factors Associated with Insomnia in Military Personnel: A Retrospective Study during the Second COVID-19 Epidemic Wave in Peru
Authors: Valladares-Garrido Mario J. , Picón-Reátegui Cinthia Karina , Zila-Velasque J. Pierre , Grados-Espinoza Pamela
Published: June 2022

Studies in military personnel are scarce and have reported increased rates of medical consultations and insomnia. The COVID-19 pandemic has been associated with a number of factors that increase the prevalence of insomnia, which has established consequences in the military. However, reported data are from different settings. We aimed to identify the prevalence and factors associated with insomnia during the second COVID-19 epidemic wave in Lambayeque, Peru. A retrospective study in 566 participants was conducted face-to-face in November 2021. The dependent variable was insomnia, measured with the Insomnia Severity Index. The independent variables were socio-labor variables, physical activity, food insecurity, eating behavior disorder, fear of COVID-19, and resilience. The prevalence of insomnia was 23% (95% CI: 19.6–26.7%). In multivariate analysis, insomnia was associated with a personal history of mental health (PR: 1.71, 95% CI: 1.01–2.93), food insecurity (PR: 1.43, 95% CI: 1.05–1.95), fear of COVID-19 (PR: 2.57, 95% CI: 1.87–3.54), and high resilience (PR: 0.60, 95% CI: 0.42–0.86). Overall, the Peruvian military population presents a high prevalence of insomnia during the pandemic period. Special attention should be paid to factors that influence insomnia. Prevention and promotion programs should be established to reverse this negative trend in the military.
Factors Associated with Length of Hospital Stay among COVID-19 Patients in Saudi Arabia: A Retrospective Study during the First Pandemic Wave
Authors: Alahmari Abdullah K. , Almalki Ziyad S. , Albassam Ahmed A. , Alsultan Mohammed M. , Alshehri Ahmed M. , Ahmed Nehad J. , Alqahtani Abdulhadi M.
Published: June 2022

The COVID-19 pandemic severely affected healthcare systems and tested their preparedness. To date, the length of hospital stay (LoHS) and its factors among COVID-19 patients has not been thoroughly studied. Moreover, it is essential to identify the features of these patients. Adult COVID-19 patients in Saudi Arabia with complete electronic medical records and who were hospitalised for >1 day between 1 May 2020 and 30 July 2020 at one of two hospitals were considered for this retrospective cohort study. Descriptive statistics and multivariate generalized linear models were performed using the data. Of the patients, 34% were ≥50 years old and 80.14% were female. More than 70% had mild-to-moderate symptoms; 45% had either diabetes or hypertension. The median LoHS was 7.00 days (IQR: 3–11). Patients who were females, had either critical or severe disease, were on mechanical ventilation, had diabetes, and administered ceftriaxone had significantly longer LoHS (p < 0.05). Patients administered zinc sulphate had significantly shorter LoHS (p = 0.0008). During the first pandemic wave, COVID-19 patients were hospitalised for 7 days. Healthcare professionals should pay more attention to women, patients with diabetes, and those with severe or critical symptoms. Unnecessary use of ceftriaxone should be minimised, and zinc sulphate can be administered.
Patient and Staff Perceptions on Using Bioelectrical Impedance Analysis in an Outpatient Haemodialysis Setting: A Qualitative Descriptive Study
Authors: Gomes Kristin , Desbrow Ben , Irwin Chris , Roberts Shelley
Published: June 2022

Bioelectrical impedance analysis (BIA) is an objective hydration and body composition assessment method recommended for use in haemodialysis patients. Limited research exists on the acceptability and utility of BIA in clinical practice. This qualitative study explored patient and staff acceptability and perceived value of BIA in an outpatient haemodialysis setting at a tertiary public hospital in Queensland, Australia. Participants included five patients receiving outpatient haemodialysis and 12 multidisciplinary clinical staff providing care to these patients. Semi-structured interviews were employed and data were analysed thematically. Patients were satisfied with the BIA measurement process and most thought the BIA data would be useful for monitoring changes in their nutrition status. Clinical staff valued BIA data for improving fluid management, assessing nutrition status and supporting patient care. Staff recommended targeting BIA use to patient groups who would benefit the most to improve its uptake in the haemodialysis setting. Conclusions: BIA use in the outpatient haemodialysis setting is acceptable and provides valuable objective data to support health-related behaviour changes in patients and enhance clinical practice. Implementation of BIA should be tailored to the local context and staff should be supported in its use.
Evaluating the Performance of Water Quality Indices: Application in Surface Water of Lake Union, Washington State-USA
Authors: Gamvroula Dimitra E. , Alexakis Dimitrios E.
Published: June 2022

Water quality indices (WQIs) are practical and versatile instruments for assessing, organizing, and disseminating information about the overall quality status of surface water bodies. The use of these indices may be beneficial in evaluating aquatic system water quality. The CCME (Canadian Council of Ministers of the Environment) and NSF (National Science Foundation) WQIs were used for the assessment of surface water (depth = 1 m) in Lake Union, Washington State. These WQIs were used in surface water at Lake Union, Seattle. The modified versions of the applied WQIs incorporate a varied number of the investigated parameters. The two WQIs were implemented utilizing specialized, publicly accessible software tools. A comparison of their performance is offered, along with a qualitative assessment of their appropriateness for describing the quality of a surface water body. Practical conclusions were generated and addressed based on the applicability and disadvantages of the evaluated indexes. When compared to the CCME-WQI, it is found that the NSF-WQI is a more robust index that yields a categorization stricter than CCME-WQI.
Community Bargaining with Urban Growth: The Case of J Village in Guangzhou
Authors: Xu Yingyan , Huang Xiaoxing
Published: June 2022

The past several decades have seen China undergo a rapid urbanization process. During periods of economic prosperity, cities expropriate outlying lands, often villages, for economic development with the support of various local and national government programs designed to encourage urban expansion. However, the autonomy of the villages has not been paid enough attention. How does incorporation into an urban development zone affect the community identity and autonomy of a village? How does the village bargain with external urban institutions? This research is based on ethnographic research and interviews conducted in 2013, 2014, 2017, and 2021. The results reveal that villagers are generally willing to accept the loss of their collective land in exchange for a larger share of the promised prosperity of industrialization, but over time they tend to find that the immediate benefits of expropriation are outweighed by long-term costs. They lose the support of the state and are exposed to new vulnerabilities, such as pollution and economic instability. Indeed, they agree to undertake unknown future risks in exchange for short-term gains. They cannot gain the right to the city, but gradually lose control of the village.
Changes of Sensory Quality, Flavor-Related Metabolites and Gene Expression in Peach Fruit Treated by Controlled Atmosphere (CA) under Cold Storage
Authors: Liu Hongru , He Hui , Liu Chenxia , Wang Chunfang , Qiao Yongjin , Zhang Bo
Published: June 2022

Controlled atmosphere (CA) has been used to alleviate chilling injury (CI) of horticultural crops caused by cold storage. However, the effects of CA treatment on peach fruit sensory quality and flavor-related chemicals suffering from CI remain largely unknown. Here, we stored peach fruit under CA with 5% O2 and 10% CO2 at 0 °C up to 28 d followed by a subsequent 3 d shelf-life at 20 °C (28S3). CA significantly reduced flesh browning and improved sensory quality at 28S3. Though total volatiles declined during extended cold storage, CA accumulated higher content of volatile esters and lactones than control at 28S3. A total of 14 volatiles were positively correlated with consumer acceptability, mainly including three C6 compounds, three esters and four lactones derived from the fatty acid lipoxygenase (LOX) pathway. Correspondingly, the expression levels of genes including PpLOX1, hyperoxide lyase PpHPL1 and alcohol acyltransferase PpAAT1 were positively correlated with the change of esters and lactones. CA elevated the sucrose content and the degree of fatty acids unsaturation under cold storage, which gave us clues to clarify the mechanism of resistance to cold stress. The results suggested that CA treatment improved sensory quality by alleviating CI of peach fruits under cold storage.
The Morphological Diversity of Antlion Larvae and Their Closest Relatives over 100 Million Years
Authors: Haug Carolin , Posada Zuluaga Victor , Zippel Ana , Braig Florian , Müller Patrick , Gröhn Carsten , Weiterschan Thomas , Wunderlich Jörg , Haug Gideon T. , Haug Joachim T.
Published: June 2022

Among lacewings (Neuroptera), representatives of the groups Ascalaphidae (owlflies) and Myrmeleontidae (antlions) are likely the most widely known ones. The exact taxonomic status of the two groups remains currently unclear, each may in fact be nested in the other group. Herein, we refer to the group including representatives of both with the neutral term “owllion”. Owllion larvae are voracious ambush hunters. They are not only known in the extant fauna, but also from the fossil record. We report here new findings of a fossil owlfly larva from Eocene Baltic amber, as well as several owlfly-like larvae from Cretaceous Kachin amber, Myanmar. Based on these fossils, combined with numerous fossil and extant specimens from the literature, collections, and databases, we compared the morphological diversity of the head and mouthpart shapes of the larvae of owllions in the extant fauna with that of owllion-like larvae from three time slices: about 100 million years ago (Cretaceous), about 40 million years ago (Eocene), and about 20 million years ago (Miocene). The comparison reveals that the samples from the Eocene and Miocene are too small for a reliable evaluation. Yet, the Cretaceous larvae allow for some conclusions: (1) the larval morphological diversity of owllion larvae increased over time, indicating a post-Cretaceous diversification; (2) certain morphologies disappeared after the Cretaceous, most likely representing ecological roles that are no longer present nowadays. In comparison, other closely related lineages, e.g., silky lacewings or split-footed lacewings, underwent more drastic losses after the Cretaceous and no subsequent diversifications.
Techniques of Primary Vaginoplasty in Young Adults with Differences of Sex Development and Female Identification
Authors: Ellerkamp Verena , Rall Kristin , Schaefer Juergen , Brucker Sara , Fuchs Joerg
Published: June 2022

Background: The ideal timing of genital surgery in differences/disorders of sex development (DSD) is controversial and differs according to the underlying type of DSD. Increasing numbers of persisting sinus as a result of delayed feminizing genitoplasty in DSD patients require interdisciplinary collaboration of pediatric surgeons/urologists and gynecologists. This study focusses on surgical techniques other than bowel vaginoplasties and results of gender assigning surgery in young adolescents. Methods: Data of adolescent and adult patients treated between 2015 and 2022 were analyzed retrospectively: underlying type of malformation, techniques of vaginoplasty, vaginal length and caliber, possibility of sexual intercourse, and temporary vaginal dilatation. Results: A total of 9 patients received a primary vaginoplasty at a median age of 16.75 years (range 10.3–29.25). The underlying anatomical conditions were persistent urogenital sinus (UGS) in 8 patients (3 patients with CAH, 2 patients with XY-DSD, 1 patient with cloacal malformation and missed UGS, 2 patients with UGS only). One patient had a MURCS association. Surgical techniques were total urogenital mobilization and perineal flap vaginoplasty in 4 patients, modified McIndoe vaginoplasty in 4 patients, and a laparoscopic vaginal pull-through in 1 patient. In a median follow-up of 45 months (2–84), all but 1 patient presented with physiological vaginal length and width. Conclusions: If possible, modern treatment concepts delay gender assigning surgery until the participation of the patient in the decision-making process is possible. Optimal treatment concepts are given by transfer of surgical techniques from pediatric urology/surgery by multidisciplinary teams. Techniques other than bowel vaginoplasties are favorable.
Prediction of the Responsiveness to Vagus-Nerve Stimulation in Patients with Drug-Resistant Epilepsy via Directed-Transfer-Function Analysis of Their Perioperative Scalp EEGs
Authors: Kim Dongyeop , Kim Taekyung , Hwang Yoonha , Lee Chae Young , Joo Eun Yeon , Seo Dae-Won , Hong Seung Bong , Shon Young-Min
Published: June 2022

This study aims to compare directed transfer function (DTF), which is an effective connectivity analysis, derived from scalp EEGs between responder and nonresponder groups implanted with vagus-nerve stimulation (VNS). Twelve patients with drug-resistant epilepsy (six responders and six nonresponders) and ten controls were recruited. A good response to VNS was defined as a reduction of ≥50% in seizure frequency compared with the presurgical baseline. DTF was calculated in five frequency bands (delta, theta, alpha, beta, and broadband) and seven grouped electrode regions (left and right frontal, temporal, parieto-occipital, and midline) in three different states (presurgical, stimulation-on, and stimulation-off states). Responders showed presurgical nodal strength close to the control group in both inflow and outflow, whereas nonresponders exhibited increased inward and outward connectivity measures. Nonresponders also had increased inward and outward connectivity measures in the various brain regions and various frequency bands assessed compared with the control group when the stimulation was on or off. Our study demonstrated that the presurgical DTF profiles of responders were different from those of nonresponders. Moreover, a presurgical normal DTF profile may predict good responsiveness to VNS.
Dynamic of Serum TWEAK Levels in Critically Ill COVID-19 Male Patients
Authors: Mikacic Marijana , Kumric Marko , Baricevic Martina , Tokic Daria , Stojanovic Stipic Sanda , Cvitkovic Ivan , Supe Domic Daniela , Ticinovic Kurir Tina , Bozic Josko
Published: June 2022

Although the number of cases and mortality of COVID-19 are seemingly declining, clinicians endeavor to establish indicators and predictors of such responses in order to optimize treatment regimens for future outbreaks of SARS-CoV-2 or similar viruses. Considering the importance of aberrant immune response in severe COVID-19, in the present study, we aimed to explore the dynamic of serum TNF-like weak inducer of apoptosis (TWEAK) levels in critically-ill COVID-19 patients and establish whether these levels may predict in-hospital mortality and if TWEAK is associated with impairment of testosterone levels observed in this population. The present single-center cohort study involved 66 men between the ages of 18 and 65 who were suffering from a severe type of COVID-19. Serum TWEAK was rising during the first week after admission to intensive care unit (ICU), whereas decline to baseline values was observed in the second week post-ICU admission (p = 0.032) but not in patients who died in hospital. Receiver-operator characteristics analysis demonstrated that serum TWEAK at admission to ICU is a significant predictor of in-hospital mortality (AUC = 0.689, p = 0.019). Finally, a negative correlation was found between serum TWEAK at admission and testosterone levels (r = −0.310, p = 0.036). In summary, serum TWEAK predicts in-hospital mortality in severe COVID-19. In addition, inflammatory pathways including TWEAK seem to be implicated in pathophysiology of reproductive hormone axis disturbance in severe form of COVID-19.