bluuebunny/crossref_metadata_2025 huggingface dataset:
Anticipatory action: Lessons for the future
Authors: Chaves-Gonzalez Juan , Milano Leonardo , Omtzigt Dirk-Jan , Pfister Daniel , Poirier Josee , Pople Ashley , Wittig Julia , Zommers Zinta
Published: December 2022

Climate shocks are causing increasingly severe damage and amplifying humanitarian needs. So far, humanitarian action has been mostly responsive, arriving after a crisis has materialized. With recent advances in forecasting, humanitarian and development organizations have been able to anticipate and respond ahead of crises. “Anticipatory action” (AA) seeks to ensure aid is provided before the peak impact of a shock occurs, reducing suffering and humanitarian needs. The UN Office for the Coordination of Humanitarian Affairs (OCHA) has been developing AA frameworks since 2019, coordinating collective AA and mobilizing finance. To date, these pilots have reached approximately 2.2 million people in Somalia, Ethiopia and Bangladesh. In six countries (Bangladesh, Burkina Faso, Malawi, Nepal, Niger, and The Philippines), frameworks are in place to reach a further 2.3 million people should the triggers be reached. OCHA is facilitating the design of AA plans in Chad, the Democratic Republic of the Congo (DRC), Madagascar, Mozambique and South Sudan. We share lessons from the pilots, focusing on three components: triggers, programming, and financing. We report that triggers must be sufficiently reliable to warrant action and funds disbursement. Forecasts are not available for all countries or hazards, and existing forecasts may not provide desired resolution or skill (accuracy) levels, especially at longer lead times. The timing of action therefore must balance forecast skill against operational needs. Funding is best when it is flexible and includes finance for framework design, evaluation and continued improvements. Finally we discuss the challenges and opportunities in scaling up AA.
Cooperative path-following control of a remotely operated underwater vehicle for human visual inspection task
Authors: Sato Eito , Liu Hailong , Orita Yasuaki , Sakagami Norimitsu , Wada Takahiro
Published: December 2022

In recent years, significant attention has been paid to remotely operated underwater vehicles (ROVs) in the performance of underwater tasks, such as the inspection and maintenance of the underwater infrastructure. The workload of ROV operators tends to be high, even for the skilled operators. Therefore, assistance methods for the operators are desired. This study focuses on a task in which a human operator controls an underwater robot to follow a certain path while visually inspecting objects in the vicinity of the path. In such a task, it is desirable to achieve the speed of trajectory control manually because the visual inspection is performed by a human operator. However, to allocate resources to visual inspection, it is desirable to minimize the workload on the path-following by assisting with the automatic control. Therefore, the objective of this study is to develop a cooperative path-following control method that achieves the above-mentioned task by expanding a robust path-following control law of non-holonomic wheeled vehicles. To simplify this problem, we considered a path-following and visual objects recognition task in a two-dimensional plane. We conducted an experiment with participants (n = 16) who completed the task using the proposed method and manual control. We compared results in terms of object recognition success rate, tracking error, completion time, attention distribution, and workload. The results showed that both the path-following errors and workload of the participants were significantly smaller with the proposed method than with manual control. In addition, subjective responses demonstrated that operator attention tended to be allocated to object recognition rather than robot operation tasks with the proposed method. These results demonstrate the effectiveness of the proposed cooperative path-following control method.
Anatomic predictor of severe prosthesis malposition following transcatheter aortic valve replacement with self- expandable Venus-A Valve among pure aortic regurgitation: A multicenter retrospective study
Authors: Wang Yong , Yu Shiyong , Qian Dehui , Li Jie , Fang Zhenfei , Cheng Wei , Li Xiaoqing , Liu Ting , Zeng Ying , Xia Hongmei , Jin Jun
Published: December 2022

Background

Transcatheter aortic valve replacement (TAVR) in the treatment of patients with pure native aortic valve regurgitation (NAVR) has been based on the “off-label” indications, while the absence of aortic valve calcification and difficulty in anchoring was found to significantly increase the risk of prosthesis malposition. The aim of this study was to explore the anatomical predictors of severe prosthesis malposition following TAVR with the self-expandable Venus-A Valve among patients with NAVR.

Methods

A total of 62 patients with NAVR who underwent TAVR with Venus-A Valve at four Chinese clinical centers were retrospectively observed. The clinical features, aortic multidetector computed tomography (MDCT) data, and clinical outcomes were compared between non-/mild malposition and severe malposition groups. Univariate logistic regression analysis was used to identify the risk factors of severe prosthesis malposition, and the receiver operating characteristic (ROC) curve was used to explore the predictive value of the risk factors.

Results

Valve migration to ascending aortic direction occurred in 1 patient, and the remaining 61 patients (including 19 severe malposition cases and 42 non-/mild malposition cases) were included in the analysis. The diameter and height of the sinotubular junction (STJ) and STJ cover index (STJCI, calculated as 100%*STJ diameter/nominal prosthesis crown diameter) were all greater in the severe malposition group (all p < 0.05). Logistic regression showed that STJ diameter (OR = 1.23, 95% CI 1.04–1.47, p = 0.017), STJ height (OR = 1.24, 95% CI 1.04–1.47, p = 0.017), and STJCI (OR = 1.08, 95% CI 1.01–1.16, p = 0.032) were potential predictors for severe prosthesis malposition. The area under the ROC curve was 0.72 (95% CI 0.58–0.85, p = 0.008) for STJ diameter, 0.70 (95% CI 0.55–0.86, p = 0.012) for STJ height, and 0.69 (95% CI 0.55–0.83, p = 0.017) for STJCI, respectively. The cutoff value was 33.2 mm for STJ diameter (sensitivity was 84.2% and specificity was 65.8%), 24.1 mm for STJ height (sensitivity was 57.9% and specificity was 87.8%), and 81.0% for STJCI (sensitivity was 68.4% and specificity was 68.3%), respectively.

Conclusion

Larger and higher STJ, as well as greater STJ to valve crown diameter ratio, may help identify patients at high risk for severe prosthesis malposition among patients with NAVR undergoing TAVR with Venus-A prosthesis valve.
Case report: Oral anticoagulant combined with percutaneous coronary intervention for peripheral embolization of left ventricular thrombus caused by myocardial infarction in a patient with diabetes mellitus
Authors: Zhu Chao , Zhou Li , Gao Hongli , Wang Jiali , Li Jiayu , Chen Hui , Li Hongwei
Published: December 2022

Background

Left ventricular thrombus (LVT) is a well-recognized complication of myocardial infarction (MI) in patients with diabetes. An embolic complication caused by LVT is a key clinical problem and is associated with worsened long-term survival.

Case presentation

A 45-year-old man with persistent left abdominal pain for 1 week and left leg fatigue was admitted to the emergency department. The cause of abdominal pain was embolism of the renal artery, the splenic artery, and the superior mesenteric artery caused by cardiogenic thrombosis, which further led to splenic infarction and renal infarction. It was unclear when MI occurred because the patient had no typical critical chest pain, which may have been related to diabetic complications, such as diabetic peripheral neuropathy. Diabetes plays a pivotal role in MI and LVT formation. Because coronary angiography suggested triple vessel disease, percutaneous transluminal coronary angioplasty (PTCA) was conducted, and two drug-eluting stents were placed in the left anterior descending coronary artery (LAD). Due to a lack of randomized clinical control trials, the therapy of LVT and associated embolization has been actively debated. According to the present guidelines, this patient was treated with low-molecular-weight heparin and warfarin (oral anticoagulants) for 3 months in addition to aspirin (100 mg/day) and clopidogrel (75 mg/day) for 1 year. No serious bleeding complications were noted, and a follow-up examination showed no thrombus in the left ventricle or further peripheral thrombotic events.

Conclusion

Peripheral embolization of LVT caused by MI leading to multiple organ embolization remains a rare occurrence. Diabetes plays a pivotal role in MI and LVT formation. Successful revascularization of the infarct-related coronary artery and anticoagulation therapy is important to minimize myocardial damage and prevent LVT. The present case will help clinicians recognize and manage LVT in patients with diabetes and related peripheral arterial thrombotic events with anticoagulation.
The role of reflection in supporting initially resistant teachers’ implementation of targeted reading instruction
Authors: Cutrer-Parraga Elizabeth A. , Miller Erica Ellsworth
Published: December 2022

One of the roles of reflection is to support teachers in implementing new instructional techniques. Collaborative reflection is a promising tool in addressing reluctance and resistance to implementing instructional techniques. This qualitative study describes how literacy coaches incorporated collaborative reflection with high implementing and initially resistant-low implementing teachers in four rural, low resourced school districts in the southeastern, United States. The findings indicated that as coaches incorporated collaborative reflection, initially resistant-low implementing teachers were more likely to share instructional needs with coaches, feel more confident in implementing the literacy intervention and express less resistance to coaching. Additional research is needed to understand collaborative reflection more fully as an aspect of the coach-teacher relationship.
The eLuna mixed-reality visual language for co-design of narrative game-based learning trails
Authors: Breien Fredrik , Wasson Barbara , Greiff Samuel , Hauan Nils Petter
Published: December 2022

Increased focus on out-of-school learning has led to extended use of Science Centers as learning arenas for junior and high school students in formal learning situations. The creation of learning trails, semantic collections of science center exhibits based on formal learning plans for interdisciplinary STEAM education, has become an area of focus. Previous design research has resulted in the definition of story-driven learning trails that foster flow and engagement in learners. In science centers, equal emphasis is placed on the physical real-world domain, represented by the exhibits themselves, as the virtual components, represented as collaborative positions-based portables carried between exhibits, linking the exhibits into virtual storylines using sensors and control assignments. This defines science center learning trails as mixed reality systems; holistic systems that integrate real and virtual elements, existing on the axis between real and virtual poles on the reality–virtuality continuum. Research has shown that a set of characteristics of narrative game-based learning has positive effects on engagement, motivation, and learning. The eLuna Framework comprises a co-design method and a visual language that emphasizes these characteristics, and that supports educators and game developers to co-specify blueprints of screen-based narrative learning game experiences. Applying thematic analysis and heuristic usability methods to interview data from two design studies completed by six science center educators based on a STEAM enabled exhibit cluster at the Bergen Science Centre VilVite, this research extends the eLuna Visual Language to distinguish between real and virtual elements for the eLuna Framework to achieve its full potential to co-design and co-specify science center mixed reality narrative game-based learning trails. The resulting extension can be plugged into the eLuna method and applied in future co-design and co-specification of mixed-reality narrative game-based learning trails which promote flow in learners, and affords positive effects on engagement, motivation, and learning.
Process-microstructure relationship of laser processed thermoelectric material Bi2Te3
Authors: Oztan Cagri , Şişik Bengisu , Welch Ryan , LeBlanc Saniya
Published: December 2022

Additive manufacturing allows fabrication of custom-shaped thermoelectric materials while minimizing waste, reducing processing steps, and maximizing integration compared to conventional methods. Establishing the process-structure-property relationship of laser additive manufactured thermoelectric materials facilitates enhanced process control and thermoelectric performance. This research focuses on laser processing of bismuth telluride (Bi2Te3), a well-established thermoelectric material for low temperature applications. Single melt tracks under various parameters (laser power, scan speed and number of scans) were processed on Bi2Te3 powder compacts. A detailed analysis of the transition in the melting mode, grain growth, balling formation, and elemental composition is provided. Rapid melting and solidification of Bi2Te3 resulted in fine-grained microstructure with preferential grain growth along the direction of the temperature gradient. Experimental results were corroborated with simulations for melt pool dimensions as well as grain morphology transitions resulting from the relationship between temperature gradient and solidification rate. Samples processed at 25 W, 350 mm/s with 5 scans resulted in minimized balling and porosity, along with columnar grains having a high density of dislocations.
Detection of oil spills based on gray level co-occurrence matrix and support vector machine
Authors: Li Kai , Yu Hongliang , Xu Yiqun , Luo Xiaoqing
Published: December 2022

Accurate reconnaissance of Marine oil spill is very important for emergency management of Marine oil spill accidents. Unmanned aerial vehicles (UAV) is a suitable carrier for offshore oil spill reconnaissance because of its fast deployment speed and low cost. Aiming at the identification accuracy of small oil spill accident in offshore port area and the problem of day and night reconnaissance, this study takes thermal infrared remote sensing images of oil leakage captured by UAV as the research object and proposes an oil spill detection method based on a Gray Level Co-occurrence Matrix (GLCM) and Support Vector Machine (SVM) method. Firstly, the extraction steps of image GLCM feature and the basic principle of SVM classification are studied. Then, the thermal infrared image data collected by UAV is preprocessed, including image filtering, clipping and rotation, and the sample database is generated. Subsequently, GLCM features of the samples were extracted, and the energy and correlation in GLCM were selected as classification features and sent to the SVM classifier to complete the oil spill detection of real-time thermal infrared images. The experimental results show that, compared with Classification and Regression Tree algorithm (CART) and Random Forests of Decision Trees (RF) algorithm, the detection accuracy of the method proposed in this paper reaches 95%, which were 10 and 2 percentage points higher than them respectively. The proposed method in this paper has fast recognition speed and high accuracy, and can provide all-weather recognition of oil spills for the detection of small oil spills in the offshore port area.
Do renewable energy consumption and financial development contribute to environmental quality in MINT nations? Implications for sustainable development
Authors: Adebayo Tomiwa Sunday , Ağa Mehmet , Agyekum Ephraim Bonah , Kamel Salah , El-Naggar Mohamed F.
Published: December 2022

Maintaining a balance between the well-being of the economy and the environment has become a top priority for governments globally. In the contemporary age, world economies, particularly the emerging ones like MINT nations, highlight the need for eco-friendly economic expansion. The MINT nations are thriving economically but are having difficulty reducing their Ecological footprint (EF). This paper aimed to determine if factors such as population density, renewable energy, foreign direct investment, economic growth, and financial development impact ecological footprint in the MINT countries between 1990 and 2018. The study applied ample advanced econometrics such as method of moments quantile regression (MMQR), augmented mean group (AMG) and Common Correlated Effects Mean Group (CCEMG). The overall results indicated that the variables are integrated at the first difference and are cointegrated. The AMG, CCEMG and MMQR results reveal that economic growth deteriorates ecological well-being by promoting the EF while foreign direct investment, population density and renewable energy assists in enhancing it by mitigating the EF in the MINT nations. In addition, financial development does not exert a significant effect on EF. The Dumitrescu Hurlin Panel Causality results show unidirectional causality from economic growth, financial development, population density, and renewable energy to EF. Based on these results policy recommendations are suggested.
Ecosystemic resilience of a temperate post-fire forest under extreme weather conditions
Authors: Blumroeder Jeanette S. , Schmidt Frederic , Gordon Anat , Grosse Stefanie , Ibisch Pierre L.
Published: December 2022

Introduction

The effects of climate change are exacerbating the fire risk in forests worldwide. Conifer plantations in particular are especially vulnerable to fire outbreaks. At the end of the extraordinarily hot and dry summer of 2018, a forest pine plantation burned in Brandenburg, NE Germany. Different forestry interventions were carried out after the fire, while one area of the damaged plantation remained untouched.

Methods

We investigated the resilience of the forest ecosystem and the effectiveness of different active and passive forest restoration measures during the subsequent relatively warm and dry years 2019–2021.

Results

One year after the fire, Populus tremula showed strong spontaneous colonization at all sites. In contrast, the majority of planted Pinus sylvestris plantlets died on the plots that had been salvage-logged after the fire. Three years after the fire, Populus tremula successfully established itself as the dominant tree species on all plots, with the highest abundance on the plot where the overstorey of the dead pines was left. Betula pendula, Salix caprea, and Pinus sylvestris showed lower abundance, with their proportion increasing with decreasing cover by dead trees. The distribution of regrowing trees is very heterogeneous across the different treatments and plots. In the clear-cut plots, the extreme microclimatic conditions expose the young trees to additional heat and drought, while the retention of deadwood measurably buffers the temperature and water stress.

Discussion

The resilience and adaptability of naturally regenerating forests that develop into ecosystems that are more diverse seem more promising than restoration through intervention. Apart from hampering restoration under extreme weather conditions, post-fire salvage logging contributes to soil degradation and loss of organic carbon.
Imaging tools for plant nanobiotechnology
Authors: Zhao Bin , Luo Zhongxu , Zhang Honglu , Zhang Huan
Published: December 2022

The successful application of nanobiotechnology in biomedicine has greatly changed the traditional way of diagnosis and treating of disease, and is promising for revolutionizing the traditional plant nanobiotechnology. Over the past few years, nanobiotechnology has increasingly expanded into plant research area. Nanomaterials can be designed as vectors for targeted delivery and controlled release of fertilizers, pesticides, herbicides, nucleotides, proteins, etc. Interestingly, nanomaterials with unique physical and chemical properties can directly affect plant growth and development; improve plant resistance to disease and stress; design as sensors in plant biology; and even be used for plant genetic engineering. Similarly, there have been concerns about the potential biological toxicity of nanomaterials. Selecting appropriate characterization methods will help understand how nanomaterials interact with plants and promote advances in plant nanobiotechnology. However, there are relatively few reviews of tools for characterizing nanomaterials in plant nanobiotechnology. In this review, we present relevant imaging tools that have been used in plant nanobiotechnology to monitor nanomaterial migration, interaction with and internalization into plants at three-dimensional lengths. Including: 1) Migration of nanomaterial into plant organs 2) Penetration of nanomaterial into plant tissues (iii)Internalization of nanomaterials by plant cells and interactions with plant subcellular structures. We compare the advantages and disadvantages of current characterization tools and propose future optimal characterization methods for plant nanobiotechnology.
ggMOB: Elucidation of genomic conjugative features and associated cargo genes across bacterial genera using genus-genus mobilization networks
Authors: Nayar Gowri , Terrizzano Ignacio , Seabolt Ed , Agarwal Akshay , Boucher Christina , Ruiz Jaime , Slizovskiy Ilya B. , Kaufman James H. , Noyes Noelle R.
Published: December 2022

Horizontal gene transfer mediated by conjugation is considered an important evolutionary mechanism of bacteria. It allows organisms to quickly evolve new phenotypic properties including antimicrobial resistance (AMR) and virulence. The frequency of conjugation-mediated cargo gene exchange has not yet been comprehensively studied within and between bacterial taxa. We developed a frequency-based network of genus-genus conjugation features and candidate cargo genes from whole-genome sequence data of over 180,000 bacterial genomes, representing 1,345 genera. Using our method, which we refer to as ggMOB, we revealed that over half of the bacterial genomes contained one or more known conjugation features that matched exactly to at least one other genome. Moreover, the proportion of genomes containing these conjugation features varied substantially by genus and conjugation feature. These results and the genus-level network structure can be viewed interactively in the ggMOB interface, which allows for user-defined filtering of conjugation features and candidate cargo genes. Using the network data, we observed that the ratio of AMR gene representation in conjugative versus non-conjugative genomes exceeded 5:1, confirming that conjugation is a critical force for AMR spread across genera. Finally, we demonstrated that clustering genomes by conjugation profile sometimes correlated well with classical phylogenetic structuring; but that in some cases the clustering was highly discordant, suggesting that the importance of the accessory genome in driving bacterial evolution may be highly variable across both time and taxonomy. These results can advance scientific understanding of bacterial evolution, and can be used as a starting point for probing genus-genus gene exchange within complex microbial communities that include unculturable bacteria. ggMOB is publicly available under the GNU licence at https://ruiz-hci-lab.github.io/ggMOB/
Genome-wide DNA methylation of Munro’s microabscess reveals the epigenetic regulation in the pathogenesis of psoriasis
Authors: Xu Xiaoqing , Zhang Yuxi , Pan Zhaobing , Zhang Xiaojing , Liu Xiaonan , Tang Lili , Zhang Xiaoguang , Zhou Fusheng , Cheng Hui
Published: December 2022

Introduction

Munro's microabscess is a typical pathological feature in the early psoriatic lesion, mainly characterized by the accumulation of neutrophils in the epidermis. DNA methylation microenvironment of Munro's microabscess and the crosstalk with transcription and its effect on neutrophils have not yet been revealed.

Methods

Performed genome-wide DNA methylation analysis and further differential methylation analysis of psoriatic skin lesions with and without Munro's microabscess from two batch samples consisting of 114 former samples in the discovery stage and 21 newly-collected samples in the validation stage. Utilized GO, MEME, and other tools to conduct downstream analysis on differentially methylated sites (DMSs). Correlation analysis of methylation level and transcriptome data was also conducted.

Results

We observed 647 overlapping DMSs associated with Munro's microabscess. Subsequently, GO pathway analysis revealed that DNA methylation might affect the physical properties associated with skin cells through focal adhesion and cellsubstrate junction and was likely to recruit neutrophils in the epidermis. Via the MEME tool, used to investigate the possible binding transcription factors (TFs) of 20 motifs around the 647 DMSs, it was found that DNA methylation regulated the binding of AP1 family members and the recruitment of neutrophils in the epidermis through the TGF-beta pathway and the TH17 pathway. Meanwhile, combined with our earlier transcriptome data, we found DNA methylation would regulate the expressions of CFDP, SIRT6, SMG6, TRAPPC9, HSD17B7, and KIAA0415, indicating these genes would potentially promote the process of Munro's microabscess.

Discussion

In conclusion, DNA methylation may affect the course of psoriasis by regulating the progression of Munro's microabscess in psoriatic skin lesions.
High ammonium recycling in an anthropogenically altered Yeongsan River Estuary, South Korea
Authors: Lee Jiyoung , An Soonmo
Published: December 2022

Regenerated nitrogen (N) cycling was studied in a turbid and nutrient-rich estuary located in the southeast region of the Yellow Sea (Yeongsan River Estuary; YRE), in order to elucidate the biogeochemical consequences of coastal development. Ammonium regeneration and potential uptake rates were measured from March 2012 to June 2013 using 15N tracer techniques. Size fractionation suggested that small-sized bacteria (<0.7 μm), rather than zooplankton, were responsible for most of the ammonium regeneration. Intermittent freshwater discharge events might have prevented stable zooplankton community development and caused the insignificant role of zooplankton in ammonium regeneration. Ammonium regeneration and potential uptake rates were relatively high (0.1–1.2 and 0.2–1.5 µmol L−1 h−1, respectively), and were highest during summer. Ammonium turnover times were shorter than water residence times throughout the study period. These results indicate that ammonium is actively recycled and is likely to supply enough N required to sustain the high primary productivity observed in the YRE (50%–450%). Reduced turbidity and increased water residence times caused by the construction of an estuarine dam in the YRE have probably resulted in the formation of optimal conditions for the high ammonium regeneration.
A reflectionless compact elliptical half Maxwell fish-eye lens designed by transformation optics
Authors: Eskandari Hossein
Published: December 2022

A two-dimensional half Maxwell fish-eye lens is compressed using a linear transformation that maps a half circle to a half ellipse. The focusing property of the lens is preserved while making the device more compact. The boundary reflections, investigated for both TE and TM polarizations, were suppressed for beams directed toward the optical axis of the lens. A designed prototype provided a scanning range of ±20° with negligible reflections. The design’s functionality was verified using COMSOL multiphysics.
Changes in the physicochemical parameters and microbial community of a new cultivar blue wheat cereal wholemeal during sourdough production
Authors: Bartkiene Elena , Starkute Vytaute , Zokaityte Egle , Klupsaite Dovile , Mockus Ernestas , Ruzauskas Modestas , Bartkevics Vadims , Borisova Anastasija , Rocha João Miguel , Ozogul Fatih , Liatukas Zilvinas , Ruzgas Vytautas
Published: December 2022

Changes in the characteristics of a new cultivar (DS8472-5) of blue wheat during wholemeal fermentation with Pediococcus acidilactici (LUHS29), Liquorilactobacillus uvarum (LUHS245), and Lactiplantibacillus plantarum (LUHS122), including acidity, microbiological and chromaticity parameters, free amino acid (FAA), gamma-aminobutyric acid (GABA), and biogenic amine (BA) contents, macro- and micro-element concentrations and fatty acid (FA) and volatile compounds (VC), were evaluated. In addition, a metagenomic analysis was performed. The lactic acid bacteria (LAB) strains used for fermentation was a significant factor in wholemeal fermentation sample pH, redness (a*) and LAB counts (p ≤ 0.05). In most of the samples, fermentation increased the FAA content in wheat wholemeal, and the highest concentration of GABA was found in DS8472-5 LUHS122 samples. Phenylethylamine (PHE) was found in all wheat wholemeal samples; however, spermidine was only detected in fermented samples and cadaverine only in DS8472-5 LUHS122. Fermented samples showed higher omega-3 and omega-6 contents and a higher number and variety of VC. Analysis of the microbial profile showed that LAB as part of the natural microbiota present in cereal grains also actively participates in fermentation processes induced by industrial bacterial cultures. Finally, all the tested LAB were suitable for DS8472-5 wheat wholemeal fermentation, and the DS8472-5 LUHS122 samples showed the lowest pH and the highest LAB viable counts (3.94, 5.80°N, and 8.92 log10 CFU/g, respectively).
Vitamin D3 eradicates Helicobacter pylori by inducing VDR-CAMP signaling
Authors: Zhang Ye , Wang Chunya , Zhang Li , Yu Jie , Yuan Wenjie , Li Lei
Published: December 2022

Background

Vitamin D3 [VitD3, 1,25 (OH)2D3] is known to have immunomodulatory and anti-microbial properties; however, its activity against Helicobacter pylori is unclear. In this study, we established H. pylori infection models in wild-type and VitD3 receptor (VDR) knockdown mice and analyzed the effects of VitD3 and their underlying mechanisms.

Methods

VDR+/+ and VDR+/– mice were intragastrically infected with the H. pylori SS1 strain. After confirmation of H. pylori infection, mice were treated with different doses of VitD3. The infection levels in stomach tissues were quantified using the colony-forming assay, and the expression levels of the VDR and cathelicidin antimicrobial peptide (CAMP) in the gastric mucosa were analyzed by immunohistochemistry and western blotting.

Results

The gastric mucosa of VDR+/– mice was more susceptible to H. pylori colonization and had lower levels of VDR and CAMP expression than that of VDR+/+ mice. H. pylori infection upregulated VDR and CAMP expression in the stomach of both wild-type and mutant mice, and VitD3 treatment resulted in further increase of VDR and CAMP levels, while significantly and dose-dependently decreasing the H. pylori colonization rate in both mouse groups, without affecting blood calcium or phosphorus levels.

Conclusion

Our data indicate that oral administration of VitD3 reduces the H. pylori colonization rate and upregulates VDR and CAMP expression in the gastric mucosa, suggesting a role for VitD3/VDR/CAMP signaling in the eradication of H. pylori in the stomach. These findings provide important insights into the mechanism underlying the anti-H. pylori activity of VitD3 and should be useful in the development of measures to eradicate H. pylori.
Bioactive compounds of Curvularia species as a source of various biological activities and biotechnological applications
Authors: Mehta Tushar , Meena Mukesh , Nagda Adhishree
Published: December 2022

Many filamentous fungi are known to produce several secondary metabolites or bioactive compounds during their growth and reproduction with sort of various biological activities. Genus Curvularia (Pleosporaceae) is a dematiaceous filamentous fungus that exhibits a facultative pathogenic and endophytic lifestyle. It contains ~213 species among which Curvularia lunata, C. geniculata, C. clavata, C. pallescens, and C. andropogonis are well-known. Among them, C. lunata is a major pathogenic species of various economical important crops especially cereals of tropical regions while other species like C. geniculata is of endophytic nature with numerous bioactive compounds. Curvularia species contain several diverse groups of secondary metabolites including alkaloids, terpenes, polyketides, and quinones. Which possess various biological activities including anti-cancer, anti-inflammatory, anti-microbial, anti-oxidant, and phytotoxicity. Several genes and gene factors are involved to carry and regulate the expression of these activities which are influenced by environmental signals. Some species of Curvularia also show negative impacts on humans and animals. Apart from their negative effects, there are some beneficial implications like production of enzymes of industrial value, bioherbicides, and source of nanoparticles is reported. Many researchers are working on these aspects all over the world but there is no review in literature which provides significant understanding about these all aspects. Thus, this review will provide significant information about secondary metabolic diversity, their biological activities and biotechnological implications of Curvularia species.
Elevated serum uric acid is associated with cognitive improvement in older American adults: A large, population-based-analysis of the NHANES database
Authors: Geng Rulin , Zhang Yu , Liu Miao , Deng Shengfeng , Ding Jingwen , Zhong Hongfei , Tu Qiuyun
Published: December 2022

Background

The many studies revealing a connection between serum uric acid (SUA) and dementia have reported conflicting results. This study sought to investigate the relations between SUA and cognitive function in older adults.

Materials and methods

The sample was 2,767 American adults aged ≥60 years from the National Health and Nutrition Examination Survey 2011–2014. Cognitive performance was evaluated by the Consortium to Establish a Registry for Alzheimer’s Disease test, animal fluency test, digit symbol substitution test, and composite z-score. Multivariate linear regression analyses were conducted to estimate the association between SUA and cognitive function.

Results

SUA level and cognitive function were significantly, positively correlated. Age significantly correlated with the association between SUA and cognitive function.

Conclusion

These findings support a connection between SUA and cognition, showing a positive link between SUA and cognitive scores among older American adults. We contend that a slight rise in uric acid within the normal range is advantageous for enhanced cognition. To confirm the precise dose-time-response relation, more tests will be needed.
Potential bioactive compounds and mechanisms of Fibraurea recisa Pierre for the treatment of Alzheimer’s disease analyzed by network pharmacology and molecular docking prediction
Authors: Wang Shishuai , Ma Yixuan , Huang Yuping , Hu Yuhui , Huang Yushan , Wu Yi
Published: December 2022

Introduction

Heat-clearing and detoxifying Chinese medicines have been documented to have anti-Alzheimer’s disease (AD) activities according to the accumulated clinical experience and pharmacological research results in recent decades. In this study, Fibraurea recisa Pierre (FRP), the classic type of Heat-clearing and detoxifying Chinese medicine, was selected as the object of research.

Methods

12 components with anti-AD activities were identified in FRP by a variety of methods, including silica gel column chromatography, multiple databases, and literature searches. Then, network pharmacology and molecular docking were adopted to systematically study the potential anti-AD mechanism of these compounds. Consequently, it was found that these 12 compounds could act on 235 anti-AD targets, of which AKT and other targets were the core targets. Meanwhile, among these 235 targets, 71 targets were identified to be significantly correlated with the pathology of amyloid beta (Aβ) and Tau.

Results and discussion

In view of the analysis results of the network of active ingredients and targets, it was observed that palmatine, berberine, and other alkaloids in FRP were the key active ingredients for the treatment of AD. Further, Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis revealed that the neuroactive ligand-receptor interaction pathway and PI3K-Akt signaling pathway were the most significant signaling pathways for FRP to play an anti-AD role. Findings in our study suggest that multiple primary active ingredients in FRP can play a multitarget anti-AD effect by regulating key physiological processes such as neurotransmitter transmission and anti-inflammation. Besides, key ingredients such as palmatine and berberine in FRP are expected to be excellent leading compounds of multitarget anti-AD drugs.
From perceptual organization to visual illusions and back
Authors: Pinna Baingio , Porcheddu Daniele , Skilters Jurgis
Published: December 2022

In modern vision science, illusions are compelling phenomena useful as tools to explore vision under limiting psychophysical conditions. Illusions manifest at least two issues that challenge scientists. The first issue is related to the definition of illusion and to the complexity of the mismatch between the geometrical/physical and the phenomenal domains. The second issue concerns two different meanings of the term “illusion,” respectively related to the demonstration of the illusion through the mismatch between domains and to the phenomenal illusoriness, i.e., the perception of something having the nature of an illusion, unreal, ambiguous, fallacious, and deceptive. In this work, we explored the notion of illusion starting from the principles of perceptual organization as described by Gestalt psychologists. On the basis of several phenomenal conditions, step by step, we suggested some new hypotheses, whose purpose was to answer the following questions: what is physical and what is phenomenal? Is there and, if any, what is the dividing line between illusions and non-illusions? Is it true that illusions are rare phenomena? Why do illusions exist? What is their perceptual and evolutionist role? These questions and the related issues were phenomenally discussed by deepening and extending the notion of perceptual organization and by exploring the biological implications of both illusions and illusoriness. On the basis of our results, the perception of illusion and illusoriness can be considered as a further challenge for vision scientists useful to shed new insights within the biological meanings of visual perception and within the no-man land between sensory and cognitive processes that elicit visual consciousness not fully explored yet.
Reviewing the role of gut microbiota in the pathogenesis of depression and exploring new therapeutic options
Authors: Han Wenjie , Wang Na , Han Mengzhen , Ban Meng , Sun Tao , Xu Junnan
Published: December 2022

The relationship between gut microbiota (GM) and mental health is one of the focuses of psychobiology research. In recent years, the microbial-gut-brain axis (MGBA) concept has gradually formed about this bidirectional communication between gut and brain. But how the GM is involved in regulating brain function and how they affect emotional disorders these mechanisms are tenuous and limited to animal research, and often controversial. Therefore, in this review, we attempt to summarize and categorize the latest advances in current research on the mechanisms of GM and depression to provide valid information for future diagnoses and therapy of mental disorders. Finally, we introduced some antidepressant regimens that can help restore gut dysbiosis, including classic antidepressants, Chinese materia medica (CMM), diet, and exogenous strains. These studies provide further insight into GM’s role and potential pathways in emotion-related diseases, which holds essential possible clinical outcomes for people with depression or related psychiatric disorders. Future research should focus on clarifying the causal role of GM in disease and developing microbial targets, applying these findings to the prevention and treatment of depression.
Human gait-labeling uncertainty and a hybrid model for gait segmentation
Authors: Wu Jiaen , Maurenbrecher Henrik , Schaer Alessandro , Becsek Barna , Awai Easthope Chris , Chatzipirpiridis George , Ergeneman Olgac , Pané Salvador , Nelson Bradley J.
Published: December 2022

Motion capture systems are widely accepted as ground-truth for gait analysis and are used for the validation of other gait analysis systems. To date, their reliability and limitations in manual labeling of gait events have not been studied.

Objectives

Evaluate manual labeling uncertainty and introduce a hybrid stride detection and gait-event estimation model for autonomous, long-term, and remote monitoring.

Methods

Estimate inter-labeler inconsistencies by computing the limits-of-agreement. Develop a hybrid model based on dynamic time warping and convolutional neural network to identify valid strides and eliminate non-stride data in inertial (walking) data collected by a wearable device. Finally, detect gait events within a valid stride region.

Results

The limits of inter-labeler agreement for key gait events heel off, toe off, heel strike, and flat foot are 72, 16, 24, and 80 ms, respectively; The hybrid model's classification accuracy for stride and non-stride are 95.16 and 84.48%, respectively; The mean absolute error for detected heel off, toe off, heel strike, and flat foot are 24, 5, 9, and 13 ms, respectively, when compared to the average human labels.

Conclusions

The results show the inherent labeling uncertainty and the limits of human gait labeling of motion capture data; The proposed hybrid-model's performance is comparable to that of human labelers, and it is a valid model to reliably detect strides and estimate the gait events in human gait data.

Significance

This work establishes the foundation for fully automated human gait analysis systems with performances comparable to human-labelers.
Intake of Lactobacillus paragasseri SBT2055 improves subjective symptoms of common cold during winter season in healthy adults: A randomized, double-blind, placebo-controlled parallel-group comparative study
Authors: Kobatake Eiji , Iwama Yoshitaka , Arai Toshinobu , Shioya Nobuhiko , Kise Mai , Kabuki Toshihide
Published: December 2022

Objective

Lactobacillus paragasseri SBT2055 (LG2055) has been reported to show immunostimulating effects. This study aimed to investigate the effects of LG2055 on the subjective symptoms of the physical condition in healthy adults.

Materials and methods

In this randomized, double-blind, placebo-controlled, parallel-group comparative study, Japanese individuals aged 20–64 years were recruited. A total of 200 participants were randomly divided into two groups by an independent controller (LG2055 and placebo groups; 100 participants per group). Drinkable yogurts containing LG2055 or lacking LG2055 (placebo) were used as test samples. The participants ingested one bottle of the test sample once a day for 12 weeks. A daily physical health questionnaire survey (about common cold symptoms) was performed as the primary outcome, and immunological and oxidative stress markers in saliva and serum were evaluated as secondary outcomes.

Results

In total, 198 participants completed the scheduled intake of the test samples, and five participants were excluded from the final analysis. Consequently, 193 participants (LG2055 group, n = 97; placebo group, n = 96) in the Per-Protocol Set were included in the efficacy analysis. The cumulative days of each symptom were evaluated, and the LG2055 group showed a significantly higher ratio of “without symptom” in runny nose, plugged nose, sneezing, sore throat, hoarseness, cough, headache, feeling tired, and fever than the placebo group, indicating that the incidence rates of common cold symptoms were lower in the LG2055 group. Additionally, changes in the salivary secretory IgA levels were significantly higher, and the serum derivatives of reactive oxygen metabolites levels were significantly lower in the LG2055 group.

Conclusion

Our study revealed that intake of LG2055 decreased common cold symptoms and improved immune parameters in healthy adults. This suggests that LG2055 contributes to the maintenance of physical conditions by improving the host immune system.

Clinical trial registration

[https://www.umin.ac.jp/ctr/index.htm], identifier [UMIN000045901].
The impact of COVID-19 pandemic on food habits and neophobia in children in the framework of the family context and parents’ behaviors: A study in an Italian central region
Authors: Di Nucci Annalisa , Scognamiglio Umberto , Grant Federica , Rossi Laura
Published: December 2022

Objective

This paper aims to evaluate whether changes in lifestyle and eating habits resulting from the Covid-19 emergency have influenced the post-pandemic level of food neophobia and in children living in an Italian central region.

Methods

A sample of 99 children took part in a retrospective assessment carried out with a self-administrated questionnaire. Pre and post-pandemic evaluation of eating habits, physical activity, and lifestyle indicators was carried out. Food neophobia was evaluated following the Child Food Neophobia Scale (CFNS). Descriptive statistics were produced. A contingency analysis was performed to check associations between variables.

Results

For a large part of the sample (97%) the selective food refusal did not change during the pandemic period. About 70% of participants did not change their eating habits, with some subgroups reporting an increase in the consumption of fruits (22.2%), vegetables (19.2%), and legumes (21.2%). Relevantly the impact of the pandemic on the sedentary attitude passed from 25.3 to 70.7%. Neophobia was not associated with ponderal status (p-value 0.5). However, in normal-weight children, a high prevalence of intermediate-level neophobia (78.4%) was found. 39.4% of the studied children were involved in meal preparation during social isolation, with an increase in the proportion of children that shared all meals with their family (32.3% vs. 78.8%). Non-coercive parent behaviors in reaction to food refusal were associated with low levels of neophobia (p-value < 0.05).

Discussion

In this sample, for the effect of parents’ attitudes, the pandemic positively affected children’s food habits and, consequently, the level of neophobia after the social restrictions. The main implication of the study is the importance of capitalizing on the period of restrictions in order to involve children in meal sharing and food preparation.