bluuebunny/crossref_metadata_2025 huggingface dataset:
Bat diversity is driven by elevation and distance to the nearest watercourse in aterra firmeforest in the northeastern Brazilian Amazon
Authors: Ferreira Marcelo Martins , Xavier Bruna da Silva , Bobrowiec Paulo Estefano Dineli , de Castro Isaí Jorge , Hilário Renato , da Cunha Alan Cavalcanti , Oliveira Leidiane Leão , Toledo José Júlio de , Carvalho William Douglas
Published: December 2022

Variations in environmental conditions along gradients play an important role in species distribution through environmental filtering of morphological and physiological traits; however, their effects on bat diversity remain poorly understood. Here, we investigate the effect of the distance to the nearest watercourse, terrain elevation, vegetation clutter, basal area and canopy height on taxonomic, functional and phylogenetic diversity and on the predominance of some functional traits (body mass, wing morphology and trophic level) of bat assemblages (phyllostomid and mormoopid bats) in aterra firmeforest, in the northeastern Brazilian Amazon. We captured bats using mist nets in 15 permanent plots over a 25 km2area of continuous forest. We captured 279 individuals belonging to 28 species with a total of 77.760 m2.h of sampling effort. Our results showed that bat richness increases as a function of distance to the nearest watercourse and that the assemblage also changes, with more diverse taxonomic and functional groups in areas further from the watercourse. Furthermore, elevation positively affects species richness, and the basal area of the forest positively influences the average body mass of bats. Taken together, our results demonstrate that subtle variations in the environmental conditions along a local scale gradient impact on the main dimensions of bat diversity in primary forests.
Thatcher's North Sea: The Return of Cheap Oil and the ‘Neo-liberalisation’ of European Energy
Authors: Garavini Giuliano
Published: December 2022

This article will focus on the prominent role played by the British Conservative government, guided since 1979 by Margaret Thatcher, in re-launching globally an energy model based on cheap fossil fuels by leveraging the newly available petroleum extracted in the North Sea. Between 1980 and 2010 global oil consumption increased by 50 per cent, while both coal and natural gas consumption nearly doubled. North Sea oil represented a crucial, if never openly acknowledged, ally for Thatcher, serving the purpose of bringing down oil prices, while at the same time achieving other crucial policy goals.The advent of the British North Sea oil weakened OPEC control of the global oil market, helped crush the resistance of the British coal miners, fed the ‘de-nationalisation’ of British energy sector, and then contributed to promote the ‘neoliberal governance’ of the EU energy sector.
Beyond colonial urbanism: state power, global connections and fragmented land regimes in twentieth-century Hyderabad city
Authors: Beverley Eric Lewis
Published: December 2022

Urban histories of modern South Asia have centred on British Indian cities and the reign of colonial urbanism, with dependence on metropolitan imperatives and models regarded as givens. Focusing on Hyderabad, one of the subcontinent's five largest cities and capital of an autonomous princely state throughout the colonial era, this article establishes the analytical utility of princely urbanism as a framework for writing the history of South Asian cities. Characterized by state-directed planning, transnational urbanist networks and multiple overlapping property regimes, this mode of city development and its resonance points to hidden genealogies of modern urbanism.
Helsinki Stock Exchange: trading and listed securities, 1912–1981
Authors: Vaihekoski Mika
Published: December 2022

A newly collected historical database for the Helsinki Stock Exchange (HSE) is used to analyse the number and structure of listed equity securities. The analysis shows that from its establishment in October 1912 to the end of 1981, a total of 849 different stock series and related issue rights have been listed on the HSE. Of these, 206 are normal stock series and they represent 167 different companies. The two largest industries represented on the HSE during most of the analysed period are metal and manufacturing and pulp and paper industries. Together they represented more than half of the listed companies throughout most of the sample period.
Congenital giant left ventricular aneurysm
Authors: Bekheet Samia , Al-Radi Osman O , Abou Zahr Riad
Published: December 2022

We report a case of congenital giant left ventricular aneurysm with severely depressed systolic cardiac function who underwent early surgical resection with subsequent recovery of left ventricular systolic function.
L1-Korean speakers’ definiteness processing in L2 English: A visual world paradigm eye tracking study
Authors: Ahn Hyunah , Song Mi-Jeong
Published: December 2022

This study investigated whether L1-Korean L2-English speakers could use (in)definiteness to predict unmentioned (non-)unique referents. In previous studies on the topic, methodological considerations made it difficult to separate the effects of grammar from the effects of non-linguistic knowledge. We used a visual world paradigm eye-tracking task to resolve such issues and designed stimuli that focused only on (in)definiteness. Participants’ eye movements were recorded as they heard “Click on the/a blue circle.” L1 and advanced L2 speakers used definiteness information to predict unique referents before the critical noun (circle) was heard while indefinite articles were not utilized to the same extent. Intermediate L2 speakers relied heavily on color words, not articles, to locate a referent. The results imply that predicting a referent solely based on definiteness (without real-world knowledge) requires substantial advancement in L2 development, and indefinite articles do not predict non-unique referents as clearly as definite articles predict unique referents.
Trying to Reverse Demographic Decline: Pro-Natalist and Family Policies in Russia, Poland and Hungary
Authors: Cook Linda J. , Iarskaia-Smirnova Elena R. , Kozlov Vladimir A.
Published: December 2022

During the early 2000s governments in Russia, Poland and Hungary declared demographic crises and adopted pro-natalist programmes to increase fertility, as well as policies to support families with children. Our article compares their ‘flagship’ pro-natalist programmes: Russia’s Maternity Capital, Poland’s Family 500+, and Hungary’s enhanced earned income tax credit, all framed by governments’ neo-familialist discourses. We confirm these policies had limited impacts on fertility, finding that their most significant and disparate effects were instead on childhood poverty. Provision of preschool public childcare and parental leaves with levels of mothers’ employment across the cases were compared. We find no relationship between coverage of childcare institutions and employment rates of mothers with young children. Data show a weak relationship between length and compensation for parental leaves and maternal employment. We conclude that post-communist governments’ flagship pro-natalist incentives, family policies and traditionalist rhetoric have had limited effects in reversing demographic decline or in re-traditionalising contemporary women’s lives.
Dentistry responding to domestic violence and abuse: a dental, practice-based intervention and a feasibility study for a cluster randomised trial
Authors: Coulthard Paul , Feder Gene , Evans Maggie A. , Johnson Medina , Walsh Tanya , Robinson Peter G. , Armitage Christopher J. , Barbosa Estela , Tickle Martin , Femi-Ajao Omolade
Published: December 2022

Objectives Assess the feasibility of using the Identification and Referral to Improve Safety (IRIS) intervention in a general dental practice setting and evaluating it using a cluster randomised trial design. IRIS is currently used in general medical practices to aid recognition and support referral into specialist support of adults presenting with injuries and other presenting factors that might have resulted from domestic violence and abuse. Also, to explore the feasibility of a cluster randomised trial design to evaluate the adapted IRIS.

Design Feasibility study for a cluster randomised trial of a practice-based intervention.

Setting Greater Manchester general dental practices.

Results It was feasible to adapt the IRIS intervention used in general medical practices to general dental practices in terms of training the clinical team and establishing a direct referral pathway to a designated advocate educator. General dental practices were keen to adopt the intervention, discuss with patients when presented with the opportunity and utilise the referral pathway. However, we could not use practice IT software prompts and data collection as for general practitioners because there is no unified dental IT system and because coding in dentistry for diagnoses, procedures and outcomes is not developed in the UK.

Conclusion While it was feasible to adapt elements of the IRIS intervention to general dental practice and there was general acceptability, we did not have enough empirical data to plan a definitive cluster randomised trial design to evaluate the IRIS-dentistry intervention within general dental practices.
Pals1 functions in redundancy with SMAP1 to inhibit Arf6 in order to prevent Rac1-dependent colorectal cancer cell migration and invasion
Authors: Harms Julia , Lüttgenau Simona Mareike , Emming Christin , Guske Justine , Weber Katrin , Wagner Thomas , Schowe Larissa , Nedvetsky Pavel , Krahn Michael P.
Published: December 2022

Downregulation of cell–cell adhesion and increased motility are prerequisites for the metastasis of cancer cells. We have recently shown that downregulation of the tight junction adapter protein Pals1 in colorectal cancer cells results in an increase of cell migration, invasion, and metastasis due to the enhanced activation of Arf6 and Rac1. We now reveal a redundancy between the Arf6-GAP SMAP1 and Pals1 in regulating Arf6 activity and thereby Rac1-dependent cell migration. The gene encoding SMAP1 is frequently disrupted in microsatellite instable colorectal cancer specimen and cell lines. In cells expressing SMAP1, deletion of Pals1 leads to disturbed formation of tight junctions but has no impact on Arf6 activity and cell migration. In contrast, inactivation of both SMAP1 and Pals1 results in enhanced Arf6/Rac1 activity and increased cell migration and invasion. Furthermore, analyzing patient cohorts, we found a significant decrease in patient’s survival when both genes were downregulated, in contrast to cases, when expression of only one of both genes was affected. Taken together, we identified a redundancy between SMAP1 and Pals1 in the regulation of activation of Arf6/Rac1, thereby controlling cell migration, invasion, and metastasis of colorectal cancer cells.
circPLIN2 promotes clear cell renal cell carcinoma progression by binding IGF2BP proteins and miR-199a-3p
Authors: Zhao Bin , Huang Cong , Pan Jie , Hu Hao , Liu Xiaojuan , Zhang Kaoyuan , Zhou Fenli , Shi Xin , Wu Jun , Yu Bo , Chen Xiaofan , Zhang Wei
Published: December 2022

Recent evidence has indicated that circular RNAs (circRNAs), a novel type of regulatory RNA, play important roles in the development and progression of various cancers. However, the potential regulatory roles and molecular mechanisms of circRNAs in clear cell renal cell carcinoma (ccRCC) remain largely unclear. Here, we explored circRNA expression profiles in 10 paired samples of RCC (including cancer tissues and surrounding tissues) from the Gene Expression Omnibus (GEO) datasets GSE124453 and GSE108735. We initially identified hsa_circ_0086457, designated circPLIN2, derived from exons 4 to 5 of the PLIN2 gene. We observed that circPLIN2 was preferentially located in the cytoplasm and was more stable than its linear counterpart PLIN2. circPLIN2 was significantly upregulated in ccRCC cells and tissues, and its overexpression was correlated with higher clinical stage and worse prognosis for ccRCC patients. Moreover, gain- and loss-of-function assays indicated that circPLIN2 promoted ccRCC cell proliferation, migration, and invasion in vitro and ccRCC tumor growth and metastasis in vivo. Mechanistically, circPLIN2 not only increased the stability of the c-Myc and MARCKSL1 mRNAs by binding to the KH domains of IGF2BP proteins but also competitively sponged miR-199a-3p to abolish the repressive effect of miR-199a-3p on ZEB1 expression, which ultimately resulted in ccRCC tumorigenesis and progression. Collectively, our results suggest that circPLIN2 may represent a promising diagnostic and prognostic biomarker and a potential therapeutic target for ccRCC patients.
Interpretable and tractable models of transcriptional noise for the rational design of single-molecule quantification experiments
Authors: Gorin Gennady , Vastola John J. , Fang Meichen , Pachter Lior
Published: December 2022

The question of how cell-to-cell differences in transcription rate affect RNA count distributions is fundamental for understanding biological processes underlying transcription. Answering this question requires quantitative models that are both interpretable (describing concrete biophysical phenomena) and tractable (amenable to mathematical analysis). This enables the identification of experiments which best discriminate between competing hypotheses. As a proof of principle, we introduce a simple but flexible class of models involving a continuous stochastic transcription rate driving a discrete RNA transcription and splicing process, and compare and contrast two biologically plausible hypotheses about transcription rate variation. One assumes variation is due to DNA experiencing mechanical strain, while the other assumes it is due to regulator number fluctuations. We introduce a framework for numerically and analytically studying such models, and apply Bayesian model selection to identify candidate genes that show signatures of each model in single-cell transcriptomic data from mouse glutamatergic neurons.
Colossal Nernst power factor in topological semimetal NbSb2
Authors: Li Peng , Qiu Pengfei , Xu Qing , Luo Jun , Xiong Yifei , Xiao Jie , Aryal Niraj , Li Qiang , Chen Lidong , Shi Xun
Published: December 2022

Today solid-state cooling technologies below liquid nitrogen boiling temperature (77 K), crucial to quantum information technology and probing quantum state of matter, are greatly limited due to the lack of good thermoelectric and/or thermomagnetic materials. Here, we report the discovery of colossal Nernst power factor of 3800 × 10−4 W m−1 K−2 under 5 T at 25 K and high Nernst figure-of-merit of 71 × 10−4 K−1 under 5 T at 20 K in topological semimetal NbSb2 single crystals. The observed high thermomagnetic performance is attributed to large Nernst thermopower and longitudinal electrical conductivity, and relatively low transverse thermal conductivity. The large and unsaturated Nernst thermopower is the result of the combination of highly desirable electronic structures of NbSb2 having compensated high mobility electrons and holes near Fermi level and strong phonon-drag effect. This discovery opens an avenue for exploring material option for the solid-state heat pumping below liquid nitrogen temperature.
Meteorin-like is an injectable peptide that can enhance regeneration in aged muscle through immune-driven fibro/adipogenic progenitor signaling
Authors: Lee David E. , McKay Lauren K. , Bareja Akshay , Li Yongwu , Khodabukus Alastair , Bursac Nenad , Taylor Gregory A. , Baht Gurpreet S. , White James P.
Published: December 2022

Pathologies associated with sarcopenia include decline in muscular strength, lean mass and regenerative capacity. Despite the substantial impact on quality of life, no pharmacological therapeutics are available to counteract the age-associated decline in functional capacity and/or, resilience. Evidence suggests immune-secreted cytokines can improve muscle regeneration, a strategy which we leverage in this study by rescuing the age-related deficiency in Meteorin-like through several in vivo add-back models. Notably, the intramuscular, peptide injection of recombinant METRNL was sufficient to improve muscle regeneration in aging. Using ex vivo media exchange and in vivo TNF inhibition, we demonstrate a mechanism of METRNL action during regeneration, showing it counteracts a pro-fibrotic gene program by triggering TNFα-induced apoptosis of fibro/adipogenic progenitor cells. These findings demonstrate therapeutic applications for METRNL to improve aged muscle, and show Fibro/Adipogenic Progenitors are viable therapeutic targets to counteract age-related loss in muscle resilience.
Vision-based Pakistani sign language recognition using bag-of-words and support vector machines
Authors: Mirza Muhammad Shaheer , Munaf Sheikh Muhammad , Azim Fahad , Ali Shahid , Khan Saad Jawaid
Published: December 2022

In order to perform their daily activities, a person is required to communicating with others. This can be a major obstacle for the deaf population of the world, who communicate using sign languages (SL). Pakistani Sign Language (PSL) is used by more than 250,000 deaf Pakistanis. Developing a SL recognition system would greatly facilitate these people. This study aimed to collect data of static and dynamic PSL alphabets and to develop a vision-based system for their recognition using Bag-of-Words (BoW) and Support Vector Machine (SVM) techniques. A total of 5120 images for 36 static PSL alphabet signs and 353 videos with 45,224 frames for 3 dynamic PSL alphabet signs were collected from 10 native signers of PSL. The developed system used the collected data as input, resized the data to various scales and converted the RGB images into grayscale. The resized grayscale images were segmented using Thresholding technique and features were extracted using Speeded Up Robust Feature (SURF). The obtained SURF descriptors were clustered using K-means clustering. A BoW was obtained by computing the Euclidean distance between the SURF descriptors and the clustered data. The codebooks were divided into training and testing using fivefold cross validation. The highest overall classification accuracy for static PSL signs was 97.80% at 750 × 750 image dimensions and 500 Bags. For dynamic PSL signs a 96.53% accuracy was obtained at 480 × 270 video resolution and 200 Bags.
An efficient human stem cells derived cardiotoxicity testing platform for testing oncotherapeutic analogues of quercetin and cinnamic acid
Authors: Mandal Saurabh , Gamit Naisarg , Biswas Subhankar , Rao C. Mallikarjun , Sethi Gautam , Warrier Sudha
Published: December 2022

Oncotherapeutics research is progressing at a rapid pace, however, not many drugs complete the successful clinical trial because of severe off-target toxicity to cardiomyocytes which ultimately leads to cardiac dysfunction. It is thus important to emphasize the need for early testing for possible cardiotoxicity of emerging oncotherapeutics. In this study, we assessed a novel stem cell-derived cardiac model for testing for cardiotoxicity of novel oncotherapeutics. We evaluated the cardiotoxic effect of synthesized derivatives of oncotherapeutics, quercetin (QMJ-2, -5, and -6) and cinnamic acid (NMJ-1, -2, and -3) using human Wharton's jelly mesenchymal stem cells-derived cardiomyocytes (WJCM) against known cardiotoxic oncologic drugs, doxorubicin, 5-fluorouracil, cisplatin. QMJ-6, NMJ-2, and NMJ-3 were not cardiotoxic and had minimum cardiac side effects. They did not show any effect on cardiomyocyte viability, caused low LDH release, and intracellular ROS production kept the calcium flux minimal and protected the active mitochondrial status in cardiomyocytes. They persevered cardiac-specific gene expression as well. However, compounds QMJ-2, QMJ-5, and NMJ-1 were cardiotoxic and the concentration needs to be reduced to prevent toxic effects on cardiomyocytes. Significantly, we were able to demonstrate that WJCM is an efficient cardiac testing model to analyze the cardiotoxicity of drugs in a human context.
Mblk-1/E93, an ecdysone related-transcription factor, targets synaptic plasticity-related genes in the honey bee mushroom bodies
Authors: Matsumura Yasuhiro , To Taiko Kim , Kunieda Takekazu , Kohno Hiroki , Kakutani Tetsuji , Kubo Takeo
Published: December 2022

Among hymenopteran insects, aculeate species such as bees, ants, and wasps have enlarged and morphologically elaborate mushroom bodies (MBs), a higher-order brain center in the insect, implying their relationship with the advanced behavioral traits of aculeate species. The molecular bases leading to the acquisition of complicated MB functions, however, remains unclear. We previously reported the constitutive and MB-preferential expression of an ecdysone-signaling related transcription factor, Mblk-1/E93, in the honey bee brain. Here, we searched for target genes of Mblk-1 in the worker honey bee MBs using chromatin immunoprecipitation sequence analyses and found that Mblk-1 targets several genes involved in synaptic plasticity, learning, and memory abilities. We also demonstrated that Mblk-1 expression is self-regulated via Mblk-1-binding sites, which are located upstream of Mblk-1. Furthermore, we showed that the number of the Mblk-1-binding motif located upstream of Mblk-1 homologs increased associated with evolution of hymenopteran insects. Our findings suggest that Mblk-1, which has been focused on as a developmental gene transiently induced by ecdysone, has acquired a novel expression pattern to play a role in synaptic plasticity in honey bee MBs, raising a possibility that molecular evolution of Mblk-1 may have partly contributed to the elaboration of MB function in insects.
In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system
Authors: Badiola-Mateos Maider , Osaki Tatsuya , Kamm Roger Dale , Samitier Josep
Published: December 2022

Proprioceptive sensory neurons (pSN) are an essential and undervalued part of the neuromuscular circuit. A protocol to differentiate healthy and amyotrophic lateral sclerosis (ALS) human neural stem cells (hNSC) into pSN, and their comparison with the motor neuron (MN) differentiation process from the same hNSC sources, facilitated the development of in vitro co-culture platforms. The obtained pSN spheroids cultured interact with human skeletal myocytes showing the formation of annulospiral wrapping-like structures between TrkC + neurons and a multinucleated muscle fibre, presenting synaptic bouton-like structures in the contact point. The comparative analysis of the genetic profile performed in healthy and sporadic ALS hNSC differentiated to pSN suggested that basal levels of ETV1, critical for motor feedback from pSN, were much lower for ALS samples and that the differences between healthy and ALS samples, suggest the involvement of pSN in ALS pathology development and progression.
Influence of online opinions and interactions on the Covid-19 vaccination in Chile
Authors: Villegas Claudio , Ortiz Abril , Arriagada Víctor , Ortega Sofía , Walker Juan , Arriagada Eduardo , Kalergis Alexis M. , Huepe Cristián
Published: December 2022

We analyze 6 months of Twitter conversations related to the Chilean Covid-19 vaccination process, in order to understand the online forces that argue for or against it and suggest effective digital communication strategies. Using AI, we classify accounts into four categories that emerge from the data as a result of the type of language used. This classification naturally distinguishes pro- and anti-vaccine activists from moderates that promote or inhibit vaccination in discussions, which also play a key role that should be addressed by public policies. We find that all categories display relatively constant opinions, but that the number of tweeting accounts grows in each category during controversial periods. We also find that accounts disfavoring vaccination tend to appear in the periphery of the interaction network, which is consistent with Chile’s high immunization levels. However, these are more active in addressing those favoring vaccination than vice-versa, revealing a potential communication problem even in a society where the antivaccine movement has no central role. Our results highlight the importance of social network analysis to understand public discussions and suggest online interventions that can help achieve successful immunization campaigns.
The usefulness of presepsin in the diagnosis of postoperative infectious complications after gastrectomy for gastric cancer: a prospective cohort study
Authors: Imai Yoshiro , Tanaka Ryo , Honda Kotaro , Matsuo Kentaro , Taniguchi Kohei , Asakuma Mitsuhiro , Lee Sang-Woong
Published: December 2022

This prospective study aimed to evaluate presepsin use as a biomarker of on postoperative infectious complications after gastrectomy, compared to C-reactive protein (CRP), white blood cells (WBCs), and neutrophils (Neuts). Overall, 108 patients were enrolled between October 2019 and December 2020. Presepsin, CRP, WBC, and Neut levels were measured preoperatively and on postoperative days (PODs) 1, 3, 5, and 7, using a postoperative morbidity survey. Grade II or higher infectious complications occurred in 18 patients (16.6%). Presepsin levels on all evaluated PODs were significantly higher in the infectious complication group than in the non-complication group (p = 0.002, p < 0.0001, p < 0.0001, and p = 0.025, respectively). The area under the curve (AUC) values were the highest for presepsin on PODs 3 and 7 (0.89 and 0.77, respectively) and similar to that of CRP, with a high value > 0.8 (0.86) on POD 5. For presepsin, the optimal cut-off values were 298 pg/mL (sensitivity, 83.3%; specificity, 83.3%), 278 pg/mL (sensitivity, 83.3%; specificity, 82.2%), and 300 pg/mL (sensitivity, 83.3%; specificity, 82%) on PODs 3, 5, and 7, respectively. Presepsin levels on PODs 3, 5, and 7 after gastrectomy is a more useful biomarker of postoperative infectious complications compared to CRP, WBCs, and Neuts, with a high sensitivity and specificity.
NLRP3 inflammasome activation in neutrophils directs early inflammatory response in murine peritonitis
Authors: Fukui Saeko , Fukui Shoichi , Van Bruggen Stijn , Shi Lai , Sheehy Casey E. , Chu Long , Wagner Denisa D.
Published: December 2022

NLR family pyrin domain containing 3 (NLRP3) inflammasome mediates caspase-1-dependent processing of inflammatory cytokines such as IL-1β, an essential endothelial activator, and contributes to the pathology of inflammatory diseases. To evaluate the role of NLRP3 in neutrophils in endothelial activation, which is still elusive, we used the thioglycollate-induced peritonitis model characterized by an early neutrophil influx, onNlrp3−/−andNlrp3+/+mice.Nlrp3−/−mice recruited fewer neutrophils thanNlrp3+/+into the peritoneum and showed lower IL-1β in peritoneal lavage fluid. The higher production of IL-1β inNlrp3+/+was neutrophil-dependent as neutrophil depletion prevented the IL-1β production. TheNlrp3+/+neutrophils collected from the peritoneal fluid formed significantly more filaments (specks) thanNlrp3−/−neutrophils of ASC (apoptosis-associated speck-like protein containing a caspase activating and recruitment domain), a readout for inflammasome activation. Intravital microscopy revealed that leukocytes rolled significantly slower inNlrp3+/+venules than inNlrp3−/−.Nlrp3−/−endothelial cells isolated from mesenteric vessels demonstrated a lower percentage of P-selectin-positive cells with lower intensity of surface P-selectin expression than theNlrp3+/+endothelial cells evaluated by flow cytometry. We conclude that neutrophils orchestrate acute thioglycollate-induced peritonitis by producing IL-1β in an NLRP3-dependent manner. This increases endothelial P-selectin expression and leukocyte transmigration.
Playing the mirror game in virtual reality with an autonomous character
Authors: Llobera Joan , Jacquat Valentin , Calabrese Carmela , Charbonnier Caecilia
Published: December 2022

Perceptual-motor synchronisation in human groups is crucial in many activities, from musical ensembles to sports teams. To this aim, the mirror game, where partners are asked to imitate each other’s movements or gestures, is one of the best available experimental paradigms to study how humans engage in joint tasks and how they tend to synchronise their behaviour. However, to date, virtual reality characters do not engage in motor synchronisation with human users. In this work, we explored to what extent an autonomous virtual character and a human that play the mirror game in virtual reality can synchronise their behaviour. We created a full-body version of the mirror game with an autonomous virtual character, whose movements were driven by a model based on coupled oscillators. Participants engaged in a joint imitation task with a virtual player animated with one of three options: a model that included a small coupling, a model with no coupling, or another human. Behavioural measures and subjective reports suggest that participants were unable to distinguish the condition of small coupling from the engagement with an avatar driven by another human participant.
Evaluation of the Access Bio CareStart rapid SARS-CoV-2 antigen test in asymptomatic individuals tested at a community mass-testing program in Western Massachusetts
Authors: Suliman Sara , Matias Wilfredo R. , Fulcher Isabel R. , Molano Francisco J. , Collins Shannon , Uceta Elizabeth , Zhu Jack , Paxton Ryan M. , Gonsalves Sean F. , Harden Maegan V. , Fisher Marissa , Meldrim Jim , Gabriel Stacey , Franke Molly F. , Hung Deborah T. , Smole Sandra C. , Madoff Lawrence C. , Ivers Louise C.
Published: December 2022

Point-of-care antigen-detecting rapid diagnostic tests (RDTs) to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) represent a scalable tool for surveillance of active SARS-CoV-2 infections in the population. Data on the performance of these tests in real-world community settings are paramount to guide their implementation to combat the COVID-19 pandemic. We evaluated the performance characteristics of the CareStart COVID-19 Antigen test (CareStart) in a community testing site in Holyoke, Massachusetts. We compared CareStart to a SARS-CoV-2 reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) reference, both using anterior nasal swab samples. We calculated the sensitivity, specificity, and the expected positive and negative predictive values at different SARS-CoV-2 prevalence estimates. We performed 666 total tests on 591 unique individuals. 573 (86%) were asymptomatic. There were 52 positive tests by RT-qPCR. The sensitivity of CareStart was 49.0% (95% Confidence Interval (CI) 34.8–63.4) and specificity was 99.5% (95% CI 98.5–99.9). Among positive RT-qPCR tests, the median cycle threshold (Ct) was significantly lower in samples that tested positive on CareStart. Using a Ct ≤ 30 as a benchmark for positivity increased the sensitivity of the test to 64.9% (95% CI 47.5–79.8). Our study shows that CareStart has a high specificity and moderate sensitivity. The utility of RDTs, such as CareStart, in mass implementation should prioritize use cases in which a higher specificity is more important, such as triage tests to rule-in active infections in community surveillance programs.
Temperature-resilient anapole modes associated with TE polarization in semiconductor nanowires
Authors: Thakore Vaibhav , Ala-Nissila Tapio , Karttunen Mikko
Published: December 2022

Polarization-dependent scattering anisotropy of cylindrical nanowires has numerous potential applications in, for example, nanoantennas, photothermal therapy, thermophotovoltaics, catalysis, sensing, optical filters and switches. In all these applications, temperature-dependent material properties play an important role and often adversely impact performance depending on the dominance of either radiative or dissipative damping. Here, we employ numerical modeling based on Mie scattering theory to investigate and compare the temperature and polarization-dependent optical anisotropy of metallic (gold, Au) nanowires with indirect (silicon, Si) and direct (gallium arsenide, GaAs) bandgap semiconducting nanowires. Results indicate that plasmonic scattering resonances in semiconductors, within the absorption band, deteriorate with an increase in temperature whereas those occurring away from the absorption band strengthen as a result of the increase in phononic contribution. Indirect-bandgap thin ($$20 \,\mathrm {nm}$$

20

nm

) Si nanowires present low absorption efficiencies for both the transverse electric (TE, $$E_{\perp }$$

E
⊥

) and magnetic (TM, $$E_{\parallel }$$

E
‖

) modes, and high scattering efficiencies for the TM mode at shorter wavelengths making them suitable as highly efficient scatterers. Temperature-resilient higher-order anapole modes with their characteristic high absorption and low scattering efficiencies are also observed in the semiconductor nanowires ($$r \! = \! 125 \! - \! 130$$

r

=

125

-

130

nm) for the TE polarization. Herein, the GaAs nanowires present $$3 \! - \! 7$$

3

-

7

times greater absorption efficiencies compared to the Si nanowires making them especially suitable for temperature-resilient applications such as scanning near-field optical microscopy (SNOM), localized heating, non-invasive sensing or detection that require strong localization of energy in the near field.
Monitoring extreme meteo-marine events in the Mediterranean area using the microseism (Medicane Apollo case study)
Authors: Borzì Alfio Marco , Minio Vittorio , Cannavò Flavio , Cavallaro Angelo , D’Amico Sebastiano , Gauci Adam , De Plaen Raphael , Lecocq Thomas , Nardone Gabriele , Orasi Arianna , Picone Marco , Cannata Andrea
Published: December 2022

Microseism is the continuous background seismic signal caused by the interaction between the atmosphere, the hydrosphere and the solid Earth. Several studies have dealt with the relationship between microseisms and the tropical cyclones, but none focused on the small-scale tropical cyclones that occur in the Mediterranean Sea, called Medicanes. In this work, we analysed the Medicane Apollo which impacted the eastern part of Sicily during the period 25 October–5 November 2021 causing heavy rainfall, strong wind gusts and violent sea waves. We investigated the microseism accompanying this extreme Mediterranean weather event, and its relationship with the sea state retrieved from hindcast maps and wave buoys. The spectral and amplitude analyses showed the space–time variation of the microseism amplitude. In addition, we tracked the position of Apollo during the time using two different methods: (i) a grid search method; (ii) an array analysis. We obtained a good match between the real position of Apollo and the location constraint by both methods. This work shows that it is possible to extract information on Medicanes from microseisms for both research and monitoring purposes.
Study on the technology of brewing red raspberry wine by using new immobilized yeast technology
Authors: Liu Miao , Qin Xiaoyu , Wu Xiaotong
Published: December 2022

The aim of the present study was to establish a new process for brewing red raspberry wine with immobilized yeast and improve the value of red raspberry. Using the spore filaments of P. chrysogenum as a carrier to co-culture with yeast to obtain immobilized yeast. And the preparation method of immobilized yeast was optimized by single-factor experiment and orthogonal experiments. And the red raspberry wine was brewed in these conditions. The result shows that the optimal preparation conditions for the immobilized yeast of P. chrysogenum were 5 g/L gluconic acid, 5 g/L yeast extract, and the addition amount of P. chrysogenum spores 1 × 105 cfu/ml. Compared with free yeast, the immobilized yeast of P. chrysogenum has better fermenting ability, can better keep the anthocyanins and polyphenols and other active ingredients of red raspberry. The quality of the wine was improved due to increase in aroma components such as alcohols and esters. The immobilized yeast of P. chrysogenum maintained good fermentation performance after three consecutive fermentations.
Visual threats reduce blood-feeding and trigger escape responses in Aedes aegypti mosquitoes
Authors: Wynne Nicole E. , Chandrasegaran Karthikeyan , Fryzlewicz Lauren , Vinauger Clément
Published: December 2022

The diurnal mosquitoesAedes aegyptiare vectors of several arboviruses, including dengue, yellow fever, and Zika viruses. To find a host to feed on, they rely on the sophisticated integration of olfactory, visual, thermal, and gustatory cues emitted by the hosts. If detected by their target, this latter may display defensive behaviors that mosquitoes need to be able to detect and escape in order to survive. In humans, a typical response is a swat of the hand, which generates both mechanical and visual perturbations aimed at a mosquito. Here, we used programmable visual displays to generate expanding objects sharing characteristics with the visual component of an approaching hand and quantified the behavioral response of female mosquitoes. Results show thatAe. aegyptiis capable of using visual information to decide whether to feed on an artificial host mimic. Stimulations delivered in a LED flight arena further reveal that landedAe. aegyptifemales display a stereotypical escape strategy by taking off at an angle that is a function of the direction of stimulus introduction. Altogether, this study demonstrates that mosquitoes landed on a host mimic can use isolated visual cues to detect and avoid a potential threat.
Application of TEM and HDRM in hydrogeophysical surveys in Meisibulake coal mine
Authors: Zhang Yanlong , Wu Yanqing , Zheng Haize , Xu Yangcheng , Cheng Xiaoyang
Published: December 2022

Water disaster is one of the major disasters threatening the safety production of coal mine, which is rank only second to gas disaster. And Meisbluke Coal Mine is seriously affected by water disasters. In order to find out the scope and location of water-bearing areas in Meisbluke Coal Mine. The comprehensive geophysical exploration method combining transient electromagnetic method (TEM) and high-density resistivity method (HDRM) is used to carry out this research. Firstly, the measuring area is determined and the relevant measuring points are arranged, and 73 TEM survey lines and 10 HDRM survey lines were arranged according to the requirements. Then, the principle, data processing method and main parameters of TEM and HDRM are introduced. The TEM detection results show that the thickness of Quaternary inferred by TEM is consistent with the geological conditions revealed by boreholes, and the thickness is about 50–80 m. And the water enrichment of the bedrock is obviously recharged by the Quaternary aquifer. Besides, the water-enriched areas in each elevation are marked and the water inrush runoff channel is deduced based on the 3D scenario inverted by TEM. And the detection results of the water-bearing areas by the two methods are in good agreement with each other, which can confirm and complement each other, and the interpretation of the data is scientific and reasonable with high reliability. Besides, the detection depth of HDRM is larger than that of TEM.
ApInAPDB: a database of apoptosis-inducing anticancer peptides
Authors: Faraji Naser , Arab Seyed Shahriar , Doustmohammadi Alireza , Daly Norelle L. , Khosroushahi Ahmad Yari
Published: December 2022

ApInAPDB (Apoptosis-Inducing Anticancer Peptides Database) consists of 818 apoptosis-inducing anticancer peptides which are manually collected from research articles. The database provides scholars with peptide related information such as function, binding target and affinity, IC50 and etc. In addition, GRAVY (grand average of hydropathy), net charge at pH 7, hydrophobicity and other physicochemical properties are calculated and presented. Another category of information are structural information includes 3D modeling, secondary structure prediction and descriptors for QSAR (quantitative structure–activity relationship) modeling. In order to facilitate the browsing process, three types of user-friendly searching tools are provided: top categories browser, simple search and advanced search. Overall ApInAPDB as the first database presenting apoptosis-inducing anticancer peptides can be useful in the field of peptide design and especially cancer therapy. Researchers can freely access the database at http://bioinf.modares.ac.ir/software/ApInAPDB/.
The El Niño Southern Oscillation drives multidirectional inter-reef larval connectivity in the Great Barrier Reef
Authors: Gurdek-Bas Rodrigo , Benthuysen Jessica A. , Harrison Hugo B. , Zenger Kyall R. , van Herwerden Lynne
Published: December 2022

The El Niño Southern Oscillation (ENSO) is the strongest source of interannual global climate variability, and extreme ENSO events are projected to increase in frequency under climate change. Interannual variability in the Coral Sea circulation has been associated with ENSO, although uncertainty remains regarding ENSO's influence on hydrodynamics and larval dispersal in the adjacent Great Barrier Reef (GBR). We investigated larval connectivity during ENSO events from 2010 to 2017 throughout the GBR, based on biophysical modelling of a widespread predatory reef fish, Lutjanus carponotatus. Our results indicate a well-connected system over the study period with high interannual variability in inter-reef connectivity associated with ENSO. Larval connectivity patterns were highly correlated to variations in the Southern Oscillation Index (SOI). During El Niño conditions and periods of weak SOI, larval dispersal patterns were predominantly poleward in the central and southern regions, reversing to a predominant equatorward flow during very strong SOI and extreme La Niña conditions. These ENSO-linked connectivity patterns were associated with positive connectivity anomalies among reefs. Our findings identify ENSO as an important source of variation in larval dispersal and connectivity patterns in the GBR, which can influence the stability of population dynamics and patterns of biodiversity in the region.
SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate
Authors: Garvin Alexander J. , Lanz Alexander J. , Morris Joanna R.
Published: December 2022

Monoclonal antibodies (MAb) to members of the Small Ubiquitin-like modifier (SUMO) family are essential tools in the study of cellular SUMOylation. However, many anti-SUMO MAbs are poorly validated, and antibody matching to detection format is without an evidence base. Here we test the specificity and sensitivity of twenty-four anti-SUMO MAbs towards monomeric and polymeric SUMO1-4 in dot-blots, immunoblots, immunofluorescence and immunoprecipitation. We find substantial variability between SUMO MAbs for different conjugation states, for detecting increased SUMOylation in response to thirteen different stress agents, and as enrichment reagents for SUMOylated RanGAP1 or KAP1. All four anti-SUMO4 monoclonal antibodies tested cross-reacted wit SUMO2/3, and several SUMO2/3 monoclonal antibodies cross-reacted with SUMO4. These data characterize the specificity of twenty-four anti-SUMO antibodies across commonly used assays, creating an enabling resource for the SUMO research community.
Benzyl trichloroacetimidates as derivatizing agents for phosphonic acids related to nerve agents by EI-GC-MS during OPCW proficiency test scenarios
Authors: Subramanian Alagu , Rosales José A. , Leif Roald N. , Valdez Carlos A.
Published: December 2022

The use of benzyl trichloroacetimidates for the benzylation of phosphonic acid nerve agent markers under neutral, basic, and slightly acidic conditions is presented. The benzyl-derived phosphonic acids were detected and analyzed by Electron Ionization Gas Chromatography–Mass Spectrometry (EI-GC–MS). The phosphonic acids used in this work included ethyl-, cyclohexyl- and pinacolyl methylphosphonic acid, first pass hydrolysis products from the nerve agents ethyl N-2-diisopropylaminoethyl methylphosphonothiolate (VX), cyclosarin (GF) and soman (GD) respectively. Optimization of reaction parameters for the benzylation included reaction time and solvent, temperature and the effect of the absence or presence of catalytic acid. The optimized conditions for the derivatization of the phosphonic acids specifically for their benzylation, included neutral as well as catalytic acid (< 5 mol%) and benzyl 2,2,2-trichloroacetimidate in excess coupled to heating the mixture to 60 °C in acetonitrile for 4 h. While the neutral conditions for the method proved to be efficient for the preparation of the p-methoxybenzyl esters of the phosphonic acids, the acid-catalyzed process appeared to provide much lower yields of the products relative to its benzyl counterpart. The method’s efficiency was tested in the successful derivatization and identification of pinacolyl methylphosphonic acid (PMPA) as its benzyl ester when present at a concentration of ~ 5 μg/g in a soil matrix featured in the Organisation for the Prohibition of Chemical Weapons (OPCW) 44th proficiency test (PT). Additionally, the protocol was used in the detection and identification of PMPA when spiked at ~ 10 μg/mL concentration in a fatty acid-rich liquid matrix featured during the 38th OPCW-PT. The benzyl derivative of PMPA was partially corroborated with the instrument's internal NIST spectral library and the OPCW central analytical database (OCAD v.21_2019) but unambiguously identified through comparison with a synthesized authentic standard. The method’s MDL (LOD) values for the benzyl and the p-methoxybenzyl pinacolyl methylphosphonic acids were determined to be 35 and 63 ng/mL respectively, while the method’s Limit of Quantitation (LOQ) was determined to be 104 and 189 ng/mL respectively in the OPCW-PT soil matrix evaluated.
Ventricular and lumbar cerebrospinal fluid analysis in 77 HIV-negative patients with Cryptococcal meningitis who received a ventriculoperitoneal shunt
Authors: Dong Qing , Huang Zhenchao , Yu Peng , Song Enpeng , Chen Zhijie , Qin Feng
Published: December 2022

Lumbar cerebrospinal fluid (CSF) parameters are widely studied and have wide clinical applications, but ventricular CSF has rarely been studied since it is relatively difficult to obtain. To determine whether there are differences between ventricular and lumbar CSF parameters and whether the differences have clinical significance, we retrospectively reviewed 77 patients with Cryptococcal meningitis who received a ventriculoperitoneal shunt. We analyzed the following parameters: white blood cell count, total protein concentration, CSF/blood glucose ratio, chloride ion concentration, and Cryptococcal count. All parameters between lumbar and ventricular CSF were remarkably different (all p < 0.001). White blood cell count, total protein level and Cryptococcal count were lower in ventricular CSF than in lumbar CSF, while CSF/blood glucose ratio and chloride ion concentration were higher. Compared to patients without ventriculomegaly, patients with ventriculomegaly had a significantly higher total protein concentration in ventricular CSF (p = 0.047). Compared to patients without surgical complications, patients with complications had a significantly lower CSF/blood glucose ratio in ventricular CSF (p = 0.032). The lumbar CSF parameters had no significant differences between these groups. The changes in lumbar CSF indices over time after shunt placement were also analyzed. After shunt placement, total protein concentration was transiently increased, white blood cell count, CSF/blood glucose ratio and chloride ion concentration were continued at the preoperative level until two months after shunting surgery. These findings suggest that the composition of ventricular CSF differs from that of lumbar CSF, and different CSF parameters have disparate rostro-caudal gradients in patients with Cryptococcal meningitis. Furthermore, ventricular and lumbar CSF parameters may have different clinical implications. Transient deterioration of lumbar CSF parameters after ventriculoperitoneal shunt placement may not be due to disease progression, but to change in CSF flow rate by CSF shunts.