bluuebunny/crossref_metadata_2025 huggingface dataset:
Mathematical Modelling of Postindustrial Land Use Value in the Big Cities in Ukraine
Authors: Openko I. , Kostyuchenko Y. V. , Tykhenko R. , Shevchenko O. , Tsvyakh O. , Ievsiukov T. , Deineha M.
Published: January 2020

The research paper deals with theoretical and methodological approaches to rational urban land use. Basing on the international scientific research, the authors offer original interpretation of the term «postindustrial land». Taking into account a drastic rapid dynamics of urbanization in large urban agglomerations worldwide and de-industrialization as a trend of urban land-use exemplified by the city of Kyiv in Ukraine a mathematical model of setting the price of postindustrial land with aimed to prediction the efficiency of industrial land transformation was developed in this study.
Heat and Mass transfer in MHD Nanofluid over a Stretching Surface along with Viscous Dissipation Effect
Authors: Govardhan K. , Narender G. , Sarma G. Sreedhar
Published: January 2020

A study of viscous dissipation effect of magnetohydrodynamic nanofluid flow passing over a stretched surface has been analyzed numerically. The formulated highly nonlinear equations for the above-mentioned flow are converted into first order ODEs. Utilizing the shooting technique along with the Adams-Bashforth Moulton Method is used to solve the BVP by using the computational software FORTRAN. The numerical results are computed by choosing different values of the involved physical parameters and compared with the earlier published results. The graphical numerical results of different physical quantities of interest are presented to analyze their dynamics under the varying physical quantities.
Latency-Bounded Target Set Selection in Signed Networks
Authors: Di Ianni Miriam , Varricchio Giovanna
Published: January 2020

It is well-documented that social networks play a considerable role in information spreading. The dynamic processes governing the diffusion of information have been studied in many fields, including epidemiology, sociology, economics, and computer science. A widely studied problem in the area of viral marketing is the target set selection: in order to market a new product, hoping it will be adopted by a large fraction of individuals in the network, which set of individuals should we “target” (for instance, by offering them free samples of the product)? In this paper, we introduce a diffusion model in which some of the neighbors of a node have a negative influence on that node, namely, they induce the node to reject the feature that is supposed to be spread. We study the target set selection problem within this model, first proving a strong inapproximability result holding also when the diffusion process is required to reach all the nodes in a couple of rounds. Then, we consider a set of restrictions under which the problem is approximable to some extent.
Pre-Exposure to an Electrical Stimulus Primes Associative Pairing of Audio and Electrical Stimuli for Dairy Heifers in a Virtual Fencing Feed Attractant Trial
Authors: Verdon Megan , Lee Caroline , Marini Danila , Rawnsley Richard
Published: January 2020

This experiment examined whether pre-exposure to an electrical stimulus from electric fencing attenuates associative pairing of audio and electrical stimuli in dairy heifers. Two treatments were applied to 30 weaned heifers naive to electric fencing. Heifers in the ‘electric-fence’ treatment were exposed to an electrified perimeter fence and two periods of strip-grazing using electrified poly-wire. Control heifers remained naïve to electric fencing. The pairing of audio and electrical stimuli was assessed in a feed attractant trial using manually controlled training collars. Heifers received an audio stimulus (2 s; 84 dB) when they breached a virtual fence after which a short electrical stimulus (0.5 s; 120 mW) was administered if they continued to move forward. If the animal stopped moving forward no further stimuli were applied. By the third training session, electric-fence heifers received a lower proportion of electrical stimuli than control heifers (p = 0.03). The more exploratory interactions a heifer had with the electric fence, the lower the proportion of electrical stimuli she received during training (rs = −0.77, p = 0.002). We conclude that experience with electrical fencing enhanced the salience of the electrical stimulus delivered by manual collars used for virtual fence training.
Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep
Authors: Mravčáková Dominika , Komáromyová Michaela , Babják Michal , Urda Dolinská Michaela , Königová Alžbeta , Petrič Daniel , Čobanová Klaudia , Ślusarczyk Sylwester , Cieslak Adam , Várady Marián , Váradyová Zora
Published: January 2020

The objective of this study is to evaluate the effect of dry wormwood and mallow on the gastrointestinal parasite of small ruminants Haemonchus contortus. Twenty-four experimentally infected lambs were randomly divided into four groups of six animals each: unsupplemented lambs, lambs supplemented with wormwood, lambs supplemented with mallow and animals supplemented with a mix of both plants. Faecal samples from the lambs were collected on day 23, 29, 36, 43, 50, 57, 64 and 75 post-infection for quantification of the number of eggs per gram (EPG). The mix of both plants contained phenolic acids (10.7 g/kg DM) and flavonoids (5.51 g/kg DM). The nematode eggs were collected and in vitro egg hatch test was performed. The aqueous extracts of both plants exhibited strong ovicidal effect on H. contortus, with ED50 and ED99 values of 1.40 and 3.76 mg/mL and 2.17 and 5.89 mg/mL, respectively, in the in vitro tests. Despite the great individual differences between the treated lambs in eggs reduction, the mean EPG of the untreated and treated groups did not differ (p > 0.05). Our results indicate that using wormwood and mallow as dietary supplements do not have a sufficient effect on lambs infected with H. contortus.
Horses Failed to Learn from Humans by Observation
Authors: Rørvang Maria Vilain , Nielsen Tina Bach , Christensen Janne Winther
Published: January 2020

Animals can acquire new behavior through both individual and social learning. Several studies have investigated horses’ ability to utilize inter-species (human demonstrator) social learning with conflicting results. In this study, we repeat a previous study, which found that horses had the ability to learn from observing humans performing an instrumental task, but we include a control for stimulus enhancement. One human demonstrator and thirty horses were included, and the horses were randomly assigned to one of three treatments: (A) full human demonstration, (B) partial human demonstration, and (C) no human demonstration. The task was for the horses to touch an object situated 1 m away from a feed box, to open this feed box, and thereby obtain a food reward. The success of each horse, the behavior directed towards the apparatus and the human, and behaviors indicative of frustration were observed. The results showed that horses observing a full and partial human demonstration were not more successful in solving the instrumental task than horses not observing any demonstration. Horses that did not solve the task expressed more box- and human-oriented behavior compared to successful horses, which may be an indication of motivation to solve the task and/or frustration from being unable to solve the task.
Vessel Trajectory Reconstruction Based on Functional Data Analysis Using Automatic Identification System Data
Authors: Jeong Myeong-Hun , Jeon Seung-Bae , Lee Tae-Young , Youm Min Kyo , Lee Dong-Ha
Published: January 2020

This study provides an automatic shipping-route construction method using functional data analysis (FDA), which analyzes information about curves, such as multiple data points over time. The proposed approach includes two steps: outlier detection and shipping-route construction. This study uses automatic-identification system (AIS) data for the experiments. The effectiveness of the proposed method is demonstrated through case studies, wherein our approach is compared with the Mahalanobis distance method for trajectory-outlier detection, and the performance of vessel trajectory reconstruction is compared with that of a density-based approach. The proposed method improves understanding of vessel-movement dynamics, thereby improving maritime monitoring and security.
Study on Degradation of Benzothiazolium-Based Ionic Liquids by UV-H2O2
Authors: Gu Jianhui , Zang Huimin , Yao Shun , Wang Xuejiao , Zhu Minghui , Song Hang
Published: January 2020

Ionic liquids (ILs) are considered to be a new type of solvent due to their low vapor pressure, some unique properties and are expected to be used widely in the following days. Given their water solubility, stable chemical structure, and biological toxicity, ILs probably reach aquatic environments and become long-term pollutants. We studied the fate of benzothiazolium-based ionic liquids catalyzed by UV-H2O2 in water. Due to the synergistic effect of UV and H2O2, the degradation efficiency can be significantly improved compared to any of them alone. Fitting results showed that the degradation reaction follows pseudo-first-order kinetics and reaction rate constants with hydroxyl radicals ranging from 0.0005 s−1 to 0.0529 s−1. The experimental data showed that satisfactory results can be obtained at a temperature of 45 ℃, an initial concentration of ionic liquid of 0.1 M, and a hydrogen peroxide concentration of 0.1 M. We also explored the effects of chloride ions and pH on degradation reactions. In an appropriate situation, the degradation yield of [C4Bth]PF6 could achieve 97% in 60 min and 99% in 90 min. The UV-H2O2 combination process could provide an effective technique for the treatment of benzothiazolium-based ionic liquids in wastewater.
Land-Use and Height of Pollen Sampling Affect Pollen Exposure in Munich, Germany
Authors: Rojo Jesús , Oteros Jose , Picornell Antonio , Ruëff Franziska , Werchan Barbora , Werchan Matthias , Bergmann Karl-Christian , Schmidt-Weber Carsten B. , Buters Jeroen
Published: January 2020

Airborne pollen concentrations vary depending on the location of the pollen trap with respect to the pollen sources. Two Hirst-type pollen traps were analyzed within the city of Munich (Germany): one trap was located 2 m above ground level (AGL) and the other one at rooftop (35 m AGL), 4.2 km apart. In general, 1.4 ± 0.5 times higher pollen amounts were measured by the trap located at ground level, but this effect was less than expected considering the height difference between the traps. Pollen from woody trees such as Alnus, Betula, Corylus, Fraxinus, Picea, Pinus and Quercus showed a good agreement between the traps in terms of timing and intensity. Similar amounts of pollen were recorded in the two traps when pollen sources were more abundant outside of the city. In contrast, pollen concentrations from Cupressaceae/Taxaceae, Carpinus and Tilia were influenced by nearby pollen sources. The representativeness of both traps for herbaceous pollen depended on the dispersal capacity of the pollen grains, and in the case of Poaceae pollen, nearby pollen sources may influence the pollen content in the air. The timing of the pollen season was similar for both sites; however, the season for some pollen types ended later at ground level probably due to resuspension processes that would favor recirculation of pollen closer to ground level. We believe measurements from the higher station provides a picture of background pollen levels representative of a large area, to which local sources add additional and more variable pollen amounts.
Emotional Attitude to Own Appearance and Appearance of the Spouse: Analysis of Relationships with the Relationship of Spouses to Themselves, Others, and the World
Authors: Vorontsova Tatyana A. , Labunskaya Vera A.
Published: January 2020

The study analyzed the relationship of attitudes toward one’s appearance and appearance of the partner with attitude toward the own personality and that of the other persons’ in married men and women. The empirical object of the study included 52 married couples in a registered (26 couples) and unregistered (26 couples) marriage with a duration from 5 months to 26 years (M = 7.31; SD = 6.78). The age of the respondents was 20–45 years old (M = 30.26; SD = 7.31; all are residents of the Russian Federation; Russians). Methods included the following questionnaires: (1) “History of the couples’ relationships”; (2) “Estimated and informative interpretation of one’s appearance and its compliance with gender–age constructs”; (3) “Color test of relationships”; (4) “Method of diagnosing interpersonal relationships”; (5) “Fundamental Interpersonal Relations Orientation-Behavior questionnaire”. Empirical data were analyzed with Spearman correlation analysis, Mann–Whitney U-test, Kruskal–Wallis H-test. The results were as follows: (1) in men and women the attitude to their appearance is related to the attitude to themselves; attitude to the spouse’s appearance is associated with the attitude to him or her; (2) in women, the assessment of their appearance is related to the attitude to their appearance; in men, the assessment of their appearance is related to the attitude to appearance of their spouses; (3) women’s attitude to their appearance is associated with the need for inclusion, while in the men’s case it is associated with the need for love; (4) men who emotionally reject their mothers are dissatisfied with the appearance of their wives.
Development of a Precision Medicine Workflow in Hematological Cancers, Aalborg University Hospital, Denmark
Authors: Bødker Julie S. , Sønderkær Mads , Vesteghem Charles , Schmitz Alexander , Brøndum Rasmus F. , Sommer Mia , Rytter Anne S. , Nielsen Marlene M. , Madsen Jakob , Jensen Paw , Pedersen Inge S. , Grubach Lykke , Severinsen Marianne T. , Roug Anne S. , El-Galaly Tarec C. , Dybkær Karen , Bøgsted Martin
Published: January 2020

Within recent years, many precision cancer medicine initiatives have been developed. Most of these have focused on solid cancers, while the potential of precision medicine for patients with hematological malignancies, especially in the relapse situation, are less elucidated. Here, we present a demographic unbiased and observational prospective study at Aalborg University Hospital Denmark, referral site for 10% of the Danish population. We developed a hematological precision medicine workflow based on sequencing analysis of whole exome tumor DNA and RNA. All steps involved are outlined in detail, illustrating how the developed workflow can provide relevant molecular information to multidisciplinary teams. A group of 174 hematological patients with progressive disease or relapse was included in a non-interventional and population-based study, of which 92 patient samples were sequenced. Based on analysis of small nucleotide variants, copy number variants, and fusion transcripts, we found variants with potential and strong clinical relevance in 62% and 9.5% of the patients, respectively. The most frequently mutated genes in individual disease entities were in concordance with previous studies. We did not find tumor mutational burden or micro satellite instability to be informative in our hematologic patient cohort.
Effect of Estrogen Receptor Status on Circulatory Immune and Metabolomics Profiles of HER2-Positive Breast Cancer Patients Enrolled for Neoadjuvant Targeted Chemotherapy
Authors: Vignoli Alessia , Muraro Elena , Miolo Gianmaria , Tenori Leonardo , Turano Paola , Di Gregorio Emanuela , Steffan Agostino , Luchinat Claudio , Corona Giuseppe
Published: January 2020

HER2-positive breast cancer (BC) represents a heterogeneous cancer disease. In an attempt to identify new stratification models useful for prognosis and therapeutic strategy, we investigated the influence of estrogen receptor (ER) status on the host immune and metabolomics profile of HER2-positive BC patients enrolled for neoadjuvant targeted chemotherapy (NATC). The study enrolled 43 HER2-positive BC patients eligible for NATC based on the trastuzumab-paclitaxel combination. Baseline circulatory cytokines and 1H NMR plasma metabolomics profiles were investigated. Differences in the immune cytokines and metabolomics profile as a function of the ER status, and their association with clinical outcomes were studied by multivariate and univariate analysis. Baseline metabolomics profiles were found to discriminate HER2-positive ER(+) from ER(−) BC patients. Within the ER(+) group an immune-metabolomics model, based on TNF-α and valine, predicted pathological complete response to NATC with 90.9% accuracy (AUROC = 0.92, p = 0.004). Moreover, metabolomics information integrated with IL-2 and IL-10 cytokine levels were prognostic of relapse with an accuracy of 95.5%. The results indicate that in HER2-positive BC patients the ER status influences the host circulatory immune-metabolomics profile. The baseline immune-metabolomics assessment in combination with ER status could represent an independent stratification tool able to predict NATC response and disease relapse of HER2-positive patients.
HDAC6—An Emerging Target Against Chronic Myeloid Leukemia?
Authors: Losson Hélène , Schnekenburger Michael , Dicato Mario , Diederich Marc
Published: January 2020

Imatinib became the standard treatment for chronic myeloid leukemia (CML) about 20 years ago, which was a major breakthrough in stabilizing the pathology and improving the quality of life of patients. However, the emergence of resistance to imatinib and other tyrosine kinase inhibitors leads researchers to characterize new therapeutic targets. Several studies have highlighted the role of histone deacetylase 6 (HDAC6) in various pathologies, including cancer. This protein effectively intervenes in cellular activities by its primarily cytoplasmic localization. In this review, we will discuss the molecular characteristics of the HDAC6 protein, as well as its overexpression in CML leukemic stem cells, which make it a promising therapeutic target for the treatment of CML.
Angiotensin-II-Evoked Ca2+ Entry in Murine Cardiac Fibroblasts Does Not Depend on TRPC Channels
Authors: Camacho Londoño Juan E. , Marx André , Kraft Axel E. , Schürger Alexander , Richter Christin , Dietrich Alexander , Lipp Peter , Birnbaumer Lutz , Freichel Marc
Published: January 2020

TRPC proteins form cation conducting channels regulated by different stimuli and are regulators of the cellular calcium homeostasis. TRPC are expressed in cardiac cells including cardiac fibroblasts (CFs) and have been implicated in the development of pathological cardiac remodeling including fibrosis. Using Ca2+ imaging and several compound TRPC knockout mouse lines we analyzed the involvement of TRPC proteins for the angiotensin II (AngII)-induced changes in Ca2+ homeostasis in CFs isolated from adult mice. Using qPCR we detected transcripts of all Trpc genes in CFs; Trpc1, Trpc3 and Trpc4 being the most abundant ones. We show that the AngII-induced Ca2+ entry but also Ca2+ release from intracellular stores are critically dependent on the density of CFs in culture and are inversely correlated with the expression of the myofibroblast marker α-smooth muscle actin. Our Ca2+ measurements depict that the AngII- and thrombin-induced Ca2+ transients, and the AngII-induced Ca2+ entry and Ca2+ release are not affected in CFs isolated from mice lacking all seven TRPC proteins (TRPC-hepta KO) compared to control cells. However, pre-incubation with GSK7975A (10 µM), which sufficiently inhibits CRAC channels in other cells, abolished AngII-induced Ca2+ entry. Consequently, we conclude the dispensability of the TRPC channels for the acute neurohumoral Ca2+ signaling evoked by AngII in isolated CFs and suggest the contribution of members of the Orai channel family as molecular constituents responsible for this pathophysiologically important Ca2+ entry pathway.
Characterization of Nano-Scale Hydroxyapatite Coating Synthesized from Eggshells Through Hydrothermal Reaction on Commercially Pure Titanium
Authors: Yu Hsing-Ning , Hsu Hsueh-Chuan , Wu Shih-Ching , Hsu Cheng-Wei , Hsu Shih-Kuang , Ho Wen-Fu
Published: January 2020

Commercially pure titanium (c.p. Ti) is often used in biomedical implants, but its surface cannot usually combine with the living bone. A coating of hydroxyapatite (HA) on the surface of titanium implants provides excellent mechanical properties and has good biological activity and biocompatibility. For optimal osteocompatibility, the structure, size, and composition of HA crystals should be closer to those of biological apatite. Our results show that the surface of c.p. Ti was entirely covered by rod-like HA nanoparticles after alkali treatment and subsequent hydrothermal treatment at 150 °C for 48 h. Nano-sized apatite aggregates began to nucleate on HA-coated c.p. Ti surfaces after immersion in simulated body fluid (SBF) for 6 h, while no obvious precipitation was found on the uncoated sample. Higher apatite-forming ability (bioactivity) could be acquired by the samples after HA coating. The HA coating featured bone-like nanostructure, high crystallinity, and carbonate substitution. It can be expected that HA coatings synthesized from eggshells on c.p. Ti through a hydrothermal reaction could be used in dental implant applications in the future.
High Homogeneity of Magnesium Doped LiNbO3 Crystals Grown by Bridgman Method
Authors: Yan Xiaodong , Tian Tian , Wang Menghui , Shen Hui , Zhou Ding , Zhang Yan , Xu Jiayue
Published: January 2020

A series of LiNbO3 crystals doped with various MgO concentrations (0, 3%, and 5 mol%) was simultaneously grown in one furnace by the modified vertical Bridgman method. The wet chemistry method was used to prepare the polycrystalline powders, and the growth conditions were optimized. The full width at half maximum of high-resolution X-ray rocking curves for (001) reflection of 5 mol% Mg doped lithium niobate (LN) crystal was about 8″, which meant it possessed high crystalline quality. The OH− absorption spectra shifted to 3534.7 cm−1, and the UV absorption edge violet shift indicated that 5 mol% MgO successfully doped in LN and exceeded the threshold. The extraordinary refractive index gradient of 5 mol% Mg doped LN crystal was as small as 2.5 × 10−5/cm, which exhibited high optical homogeneity.
Species Composition and Distribution of Jellyfish in a Seasonally Hypoxic Estuary, Hood Canal, Washington
Authors: Herrmann BethElLee , Keister Julie E.
Published: January 2020

Seasonal hypoxia (≤2 mg dissolved oxygen L−1) can have detrimental effects on marine food webs. Recent studies indicate that some jellyfish can tolerate low oxygen and may have a competitive advantage over other zooplankton and fishes in those environments. We assessed community structure and distributions of cnidarian and ctenophore jellyfish in seasonally hypoxic Hood Canal, WA, USA, at four stations that differed in oxygen conditions. Jellyfish were collected in June through October 2012 and 2013 using full-water-column and discrete-depth net tows, concurrent with CTD casts to measure dissolved oxygen (DO). Overall, southern, more hypoxic, regions of Hood Canal had higher abundances and higher diversity than the northern regions, particularly during the warmer and more hypoxic year of 2013. Of fifteen species identified, the most abundant—the siphonophore Muggiaea atlantica and hydrozoan Aglantha digitale—reached peak densities > 1800 Ind m−3 and 38 Ind m−3, respectively. M. atlantica were much more abundant at the hypoxic stations, whereas A. digitale were also common in the north. Vertical distributions explored during hypoxia showed that jellyfish were mostly in the upper 10 m regardless of the oxycline depth. Moderate hypoxia seemed to have no detrimental effect on jellyfish in Hood Canal, and may have resulted in high population densities, which could influence essential fisheries and trophic energy flow.
Niche Complementarity and Resistance to Grazing Promote the Invasion Success of Sargassum horneri in North America
Authors: Marks Lindsay M. , Reed Daniel C. , Holbrook Sally J.
Published: January 2020

Invasive species are a growing threat to conservation in marine ecosystems, yet we lack a predictive understanding of ecological factors that influence the invasiveness of exotic marine species. We used surveys and manipulative experiments to investigate how an exotic seaweed, Sargassum horneri, interacts with native macroalgae and herbivores off the coast of California. We asked whether the invasion (i.e., the process by which an exotic species exhibits rapid population growth and spread in the novel environment) of S. horneri is influenced by three mechanisms known to affect the invasion of exotic plants on land: competition, niche complementarity and herbivory. We found that the removal of S. horneri over 3.5 years from experimental plots had little effect on the biomass or taxonomic richness of the native algal community. Differences between removal treatments were apparent only in spring at the end of the experiment when S. horneri biomass was substantially higher than in previous sampling periods. Surveys across a depth range of 0–30 m revealed inverse patterns in the biomass of S. horneri and native subcanopy-forming macroalgae, with S. horneri peaking at intermediate depths (5–20 m) while the aggregated biomass of native species was greatest at shallow (<5 m) and deeper (>20 m) depths. The biomass of S. horneri and native algae also displayed different seasonal trends, and removal of S. horneri from experimental plots indicated the seasonality of native algae was largely unaffected by fluctuations in S. horneri. Results from grazing assays and surveys showed that native herbivores favor native kelp over Sargassum as a food source, suggesting that reduced palatability may help promote the invasion of S. horneri. The complementary life histories of S. horneri and native algae suggest that competition between them is generally weak, and that niche complementarity and resistance to grazing are more important in promoting the invasion success of S. horneri.
Diagnosis of the Trigeminal Nerve Injury in a Patient with Pontine Hemorrhage
Authors: Choi Eun Bi , Seo Jeong Pyo , Jang Sung Ho
Published: January 2020

Herein, we present a patient who was diagnosed with trigeminal nerve injury following a pontine hemorrhage. A 38-year-old male was diagnosed with a left pontine hemorrhage and underwent conservative management at the neurosurgery department of a university hospital. After hemorrhage onset, he felt facial pain on the right side. After seven years, he visited the rehabilitation department of another hospital for evaluation of his right facial pain. He complained of somatosensory impairment and facial pain (tingling and cold sensation) on the right side as well as difficulty chewing and gait disturbance. On neurological examination, decreased touch sensation (approximately 30%) was observed on the right side of the face, in the oral cavity, and on the tongue (anterior two-thirds) as well as weakness of the right-sided masseter muscles. He also exhibitedallodynia without dysesthesia on the right side of the face. Diffusion tensor tractography showed the right trigeminal nerve to be discontinued at the anterior margin of the pons (arrow) compared to the state of the left trigeminal nerve.
Thermodynamical Extension of a Symplectic Numerical Scheme with Half Space and Time Shifts Demonstrated on Rheological Waves in Solids
Authors: Fülöp Tamás , Kovács Róbert , Szücs Mátyás , Fawaier Mohammad
Published: January 2020

On the example of the Poynting–Thomson–Zener rheological model for solids, which exhibits both dissipation and wave propagation, with nonlinear dispersion relation, we introduce and investigate a finite difference numerical scheme. Our goal is to demonstrate its properties and to ease the computations in later applications for continuum thermodynamical problems. The key element is the positioning of the discretized quantities with shifts by half space and time steps with respect to each other. The arrangement is chosen according to the spacetime properties of the quantities and of the equations governing them. Numerical stability, dissipative error, and dispersive error are analyzed in detail. With the best settings found, the scheme is capable of making precise and fast predictions. Finally, the proposed scheme is compared to a commercial finite element software, COMSOL, which demonstrates essential differences even on the simplest—elastic—level of modeling.
A Novel Color Image Encryption Algorithm Based on Hyperchaotic Maps and Mitochondrial DNA Sequences
Authors: Mohamed Heba G. , ElKamchouchi Dalia H. , Moussa Karim H.
Published: January 2020

Multimedia encryption innovation is one of the primary ways of securely and privately guaranteeing the security of media transmission. There are many advantages when utilizing the attributes of chaos, for example, arbitrariness, consistency, ergodicity, and initial condition affectability, for any covert multimedia transmission. Additionally, many more benefits can be introduced with the exceptional space compliance, unique information, and processing capability of real mitochondrial deoxyribonucleic acid (mtDNA). In this article, color image encryption employs a confusion process based on a hybrid chaotic map, first to split each channel of color images into n-clusters; then to create global shuffling over the whole image; and finally, to apply intrapixel shuffling in each cluster, which results in very disordered pixels in the encrypted image. Then, it utilizes the rationale of human mitochondrial genome mtDNA to diffuse the previously confused pixel values. Hypothetical examination and trial results demonstrate that the anticipated scheme exhibits outstanding encryption, as well as successfully opposes chosen/known plain text, statistical, and differential attacks.
A Review of The Methanol Economy: The Fuel Cell Route
Authors: Simon Araya Samuel , Liso Vincenzo , Cui Xiaoti , Li Na , Zhu Jimin , Sahlin Simon Lennart , Jensen Søren Højgaard , Nielsen Mads Pagh , Kær Søren Knudsen
Published: January 2020

This review presents methanol as a potential renewable alternative to fossil fuels in the fight against climate change. It explores the renewable ways of obtaining methanol and its use in efficient energy systems for a net zero-emission carbon cycle, with a special focus on fuel cells. It investigates the different parts of the carbon cycle from a methanol and fuel cell perspective. In recent years, the potential for a methanol economy has been shown and there has been significant technological advancement of its renewable production and utilization. Even though its full adoption will require further development, it can be produced from renewable electricity and biomass or CO2 capture and can be used in several industrial sectors, which make it an excellent liquid electrofuel for the transition to a sustainable economy. By converting CO2 into liquid fuels, the harmful effects of CO2 emissions from existing industries that still rely on fossil fuels are reduced. The methanol can then be used both in the energy sector and the chemical industry, and become an all-around substitute for petroleum. The scope of this review is to put together the different aspects of methanol as an energy carrier of the future, with particular focus on its renewable production and its use in high-temperature polymer electrolyte fuel cells (HT-PEMFCs) via methanol steam reforming.
Genetic Improvement of Pinus koraiensis in China: Current Situation and Future Prospects
Authors: Li Xiang , Liu Xiao-Ting , Wei Jia-Tong , Li Yan , Tigabu Mulualem , Zhao Xi-Yang
Published: January 2020

Pinus koraiensis (Sieb.et Zucc) is an economically and ecologically important tree species, naturally distributed in northeastern China. Conservation efforts and genetic improvement for this species began in the 1960s and 1980s, with the establishment of several primary seed orchards based on range-wide provenance evaluations. The original breeding objective was to improve growth and wood yield, but during the recent decade, it was redefined to include other traits, such as an enhancement of wood properties, seed oil content, cone yield, and the development of elite provenance with families, clones, and varieties with good tolerance to biotic and abiotic stresses. However, improvement processes are slow due to a long breeding cycle, and the number of improved varieties is still low. In this review, we summarize the recent progress in the selective improvement of P. koraiensis varieties, such as elite provenance, family, and clones, using various breeding procedures. We collate information on advances in the improvement of P. koraiensis, based on conventional breeding and molecular marker-assisted breeding methods; identify gaps in our understanding of the tree improvement processes; and propose future research directions, which will provide new insight for subsequent genetic breeding research on P. koraiensis.
Old-Growth Forest Disturbance in the Ukrainian Carpathians
Authors: Spracklen Benedict D. , Spracklen Dominick V.
Published: January 2020

Human activity has greatly reduced the area of old-growth forest in Europe, with some of the largest remaining fragments in the Carpathian Mountains of south-western Ukraine. We used satellite image analysis to calculate old-growth forest disturbance in this region from 2010 to 2019. Over this period, we identified 1335 ha of disturbance in old-growth forest, equivalent to 1.8% of old-growth forest in the region. During 2015 to 2019, the average annual disturbance rate was 0.34%, varying with altitude, distance to settlements and location within the region. Disturbance rates were 7–8 times lower in protected areas compared to outside of protected areas. Only one third of old-growth forest is currently within protected areas; expansion of the protected area system to include more old-growth forests would reduce future loss. A 2017 law that gave protection to all old-growth forest in Ukraine had no significant impact on disturbance rates in 2018, but in 2019 disturbance rates reduced to 0.19%. Our analysis is the first indication that this new legislation may be reducing loss of old-growth forest in Ukraine.
Spatial Patterns and Interspecific Associations During Natural Regeneration in Three Types of Secondary Forest in the Central Part of the Greater Khingan Mountains, Heilongjiang Province, China
Authors: Zhang Lingyu , Dong Lingbo , Liu Qiang , Liu Zhaogang
Published: January 2020

Seedling and sapling spatial patterns are important in community regeneration, and understanding the natural regeneration mechanisms of tree species in relation to spatial patterns will help improve forest management and community restoration efficiency. Based on data from three fixed plots established in birch forests (BF), coniferous and broadleaved mixed forests (CBMF) and coniferous mixed forests (CMF) in the central Greater Khingan Mountains in Heilongjiang Province, China, in 2017, we used the univariate and bivariate O-ring functions of the point pattern analysis method to evaluate the spatial patterns and associations of the main tree species in these three forest types at different development stages and identified the community successional stages according to the interspecific associations between dominant tree species and other tree species. The results showed that Betula platyphylla and Larix gmelinii in BF exhibited identical spatial distribution patterns and had a tendency to transition from an aggregated to a random distribution from saplings to adult trees, whereas every tree type in CBMF generally showed a random distribution. Adult trees of the main tree species in CMF, i.e., L. gmelinii and Picea koraiensis, mainly showed a random distribution, but P. koraiensis at other size classes generally showed an aggregated distribution. The intraspecific associations of the main tree species in BF and CMF at different development stages were constrained by the spatial scale within a given scope, while those in CBMF at different development stages were not significantly constrained by spatial scale. The results also show that the density of the three forest types was affected by the distance between the individuals of the various tree classes and adult trees, with different levels of influence. We analyzed the interspecific associations between dominant tree species and other tree species and then assessed community succession progress and found that the BF and CMF exhibited medium-term community succession, while the CBMF was in the primary stage. Our results further show that the spatial distribution patterns of the tree species in the small-diameter classes were jointly affected by adjacent habitats and diffusional limitations and that scale dependence existed in the intraspecific and interspecific associations. The analysis of the natural regeneration of spatial distribution and interspecific associations represents an efficient way to explore the stability of forest communities and dynamic changes in interspecific relationships during succession. The study results thus provide a theoretical basis for developing rational forest management measures.
Poly(Hydroxyethyl Methacrylate) Immunoaffinity Cryogel Column for the Purification of Human Immunoglobulin M
Authors: Bakhshpour Monireh , Topcu Aykut Arif , Bereli Nilay , Alkan Huseyin , Denizli Adil
Published: January 2020

Human immunoglobulin M (hIgM) antibodies are considered as hopeful tools for diseases therapy. Therefore, chromatography approaches are used to purify hIgM with a single step. In this study, we prepared a poly(hydroxyethyl methacrylate) based immunoaffinity p(HEMA-I) cryogel column by using cyanamide to immobilize antihuman immunoglobulin on the p(HEMA) cryogel for purification of hIgM in aqueous solution and artificial human plasma. The characterization of the p(HEMA) cryogel column was performed by using a scanning electron microscope (SEM), micro-computerized tomography (µ-CT), Fourier transform infrared spectroscopy (FTIR), swelling degree and macro-porosity. Further, the optimizations of various parameters were performed such as, pH, ionic strength, temperature and concentration of hIgM in aqueous solutions. In addition, the Langmuir adsorption model was supported by experimental results. Maximum adsorbed amount of hIgM corresponded to 11.1 mg/g at pH 5.75 [morpholino ethanesulfonic acid (MES buffer)]. Our results indicated that the p(HEMA-I) cryogel column can be reused at least 10 times without significant loss in adsorption capacity. As a natural source, artificial human plasma was selected for hIgM adsorption and the purity of hIgM was evaluated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Comprehensive Geno- and Phenotyping in a Complex Pedigree Including Four Different Inherited Retinal Dystrophies
Authors: Birtel Johannes , Gliem Martin , Hess Kristina , Birtel Theresa H. , Holz Frank G. , Zechner Ulrich , Bolz Hanno J. , Herrmann Philipp
Published: January 2020

Inherited retinal dystrophies (IRDs) are characterized by high clinical and genetic heterogeneity. A precise characterization is desirable for diagnosis and has impact on prognosis, patient counseling, and potential therapeutic options. Here, we demonstrate the effectiveness of the combination of in-depth retinal phenotyping and molecular genetic testing in complex pedigrees with different IRDs. Four affected Caucasians and two unaffected relatives were characterized including multimodal retinal imaging, functional testing, and targeted next-generation sequencing. A considerable intrafamilial phenotypic and genotypic heterogeneity was identified. While the parents of the index family presented with rod-cone dystrophy and ABCA4-related retinopathy, their two sons revealed characteristics in the spectrum of incomplete congenital stationary night blindness and ocular albinism, respectively. Molecular testing revealed previously described variants in RHO, ABCA4, and MITF as well as a novel variant in CACNA1F. Identified variants were verified by intrafamilial co-segregation, bioinformatic annotations, and in silico analysis. The coexistence of four independent IRDs caused by distinct mutations and inheritance modes in one pedigree is demonstrated. These findings highlight the complexity of IRDs and underscore the need for the combination of extensive molecular genetic testing and clinical characterization. In addition, a novel variant in the CACNA1F gene is reported associated with incomplete congenital stationary night blindness.
Control of DNA Damage Bypass by Ubiquitylation of PCNA
Authors: Ripley Brittany M. , Gildenberg Melissa S. , Washington M. Todd
Published: January 2020

DNA damage leads to genome instability by interfering with DNA replication. Cells possess several damage bypass pathways that mitigate the effects of DNA damage during replication. These pathways include translesion synthesis and template switching. These pathways are regulated largely through post-translational modifications of proliferating cell nuclear antigen (PCNA), an essential replication accessory factor. Mono-ubiquitylation of PCNA promotes translesion synthesis, and K63-linked poly-ubiquitylation promotes template switching. This article will discuss the mechanisms of how these post-translational modifications of PCNA control these bypass pathways from a structural and biochemical perspective. We will focus on the structure and function of the E3 ubiquitin ligases Rad18 and Rad5 that facilitate the mono-ubiquitylation and poly-ubiquitylation of PCNA, respectively. We conclude by reviewing alternative ideas about how these post-translational modifications of PCNA regulate the assembly of the multi-protein complexes that promote damage bypass pathways.
An Experimental Hut Evaluation of PBO-Based and Pyrethroid-Only Nets against the Malaria Vector Anopheles funestus Reveals a Loss of Bed Nets Efficacy Associated with GSTe2 Metabolic Resistance
Authors: Menze Benjamin D. , Kouamo Mersimine F. , Wondji Murielle J. , Tchapga Williams , Tchoupo Micareme , Kusimo Michael O. , Mouhamadou Chouaibou S. , Riveron Jacob M. , Wondji Charles S.
Published: January 2020

Growing insecticide resistance in malaria vectors is threatening the effectiveness of insecticide-based interventions, including Long Lasting Insecticidal Nets (LLINs). However, the impact of metabolic resistance on the effectiveness of these tools remains poorly characterized. Using experimental hut trials and genotyping of a glutathione S-transferase resistance marker (L119F-GSTe2), we established that GST-mediated resistance is reducing the efficacy of LLINs against Anopheles funestus. Hut trials performed in Cameroon revealed that Piperonyl butoxide (PBO)-based nets induced a significantly higher mortality against pyrethroid resistant An. funestus than pyrethroid-only nets. Blood feeding rate and deterrence were significantly higher in all LLINs than control. Genotyping the L119F-GSTe2 mutation revealed that, for permethrin-based nets, 119F-GSTe2 resistant mosquitoes have a greater ability to blood feed than susceptible while the opposite effect is observed for deltamethrin-based nets. For Olyset Plus, a significant association with exophily was observed in resistant mosquitoes (OR = 11.7; p < 0.01). Furthermore, GSTe2-resistant mosquitoes (cone assays) significantly survived with PermaNet 2.0 (OR = 2.1; p < 0.01) and PermaNet 3.0 (side) (OR = 30.1; p < 0.001) but not for Olyset Plus. This study shows that the efficacy of PBO-based nets (e.g., blood feeding inhibition) against pyrethroid resistant malaria vectors could be impacted by other mechanisms including GST-mediated metabolic resistance not affected by the synergistic action of PBO. Mosaic LLINs incorporating a GST inhibitor (diethyl maleate) could help improve their efficacy in areas of GST-mediated resistance.
Magma Fracking Beneath Active Volcanoes Based on Seismic Data and Hydrothermal Activity Observations
Authors: Kiryukhin Alexey , Chernykh Evgenia , Polyakov Andrey , Solomatin Alexey
Published: January 2020

Active volcanoes are associated with microearthquake (MEQ) hypocenters that form plane-oriented cluster distributions. These are faults delineating a magma injection system of dykes and sills. The Frac-Digger program was used to track fracking faults in the Kamchatka active volcanic belt and fore-arc region of Russia. In the case of magma laterally injected from volcanoes into adjacent structures, high-temperature hydrothermal systems arise, for example at Mutnovsky and Koryaksky volcanoes. Thermal features adjacent to these active volcanoes respond to magma injection events by degassing CO2 and by transient temperature changes. Geysers created by CO2-gaslift activity in silicic volcanism areas also flag magma and CO2 recharge and redistributions, for example at the Uzon-Geyserny, Kamchatka, Russia and Yellowstone, USA magma hydrothermal systems. Seismogenic faults in the Kamchatka fore-arc region are indicators of geofluid fracking; those faults can be traced down to 250 km depth, which is within the subduction slab below primary magma sources.
Change in Soil Particle Size Distribution and Erodibility with Latitude and Vegetation Restoration Chronosequence on the Loess Plateau, China
Authors: Zhai Jiaying , Song Yahui , Entemake Wulan , Xu Hongwei , Wu Yang , Qu Qing , Xue Sha
Published: January 2020

Analyzing the dynamics of soil particle size distribution (PSD) and erodibility is important for understanding the changes of soil texture and quality after cropland abandonment. This study aimed to determine how restoration age and latitude affect soil erodibility and the multifractal dimensions of PSD during natural recovery. We collected soil samples from grassland, shrubland, and forests with different restoration ages in the steppe zone (SZ), forest-steppe zone (FSZ), and forest zone (FZ). Various analyses were conducted on the samples, including multifractal analysis and erodibility analysis. Our results showed that restoration age had no significant effect on the multifractal dimensions of PSD (capacity dimension (D0), information dimension (D1), information dimension/capacity dimension ratio (D1/D0), correlation dimension (D2)), and soil erodibility. Multifractal dimensions tended to increase, while soil erodibility tended to decrease, with restoration age. Latitude was negatively correlated with fractal dimensions (D0, D2) and positively correlated with K and D1/D0. During vegetation restoration, restoration age, precipitation, and temperature affect the development of vegetation, resulting in differences in soil organic carbon, total nitrogen, soil texture, and soil enzyme activity, and by affecting soil structure to change the soil stability. This study revealed the impact of restoration age and latitude on soil erosion in the Loess Plateau.