bluuebunny/crossref_metadata_2025 huggingface dataset:
Doped Zinc Oxide Nanoparticles: Synthesis, Characterization and Potential Use in Nanomedicine
Authors: Carofiglio Marco , Barui Sugata , Cauda Valentina , Laurenti Marco
Published: July 2020

Smart nanoparticles for medical applications have gathered considerable attention due to an improved biocompatibility and multifunctional properties useful in several applications, including advanced drug delivery systems, nanotheranostics and in vivo imaging. Among nanomaterials, zinc oxide nanoparticles (ZnO NPs) were deeply investigated due to their peculiar physical and chemical properties. The large surface to volume ratio, coupled with a reduced size, antimicrobial activity, photocatalytic and semiconducting properties, allowed the use of ZnO NPs as anticancer drugs in new generation physical therapies, nanoantibiotics and osteoinductive agents for bone tissue regeneration. However, ZnO NPs also show a limited stability in biological environments and unpredictable cytotoxic effects thereof. To overcome the abovementioned limitations and further extend the use of ZnO NPs in nanomedicine, doping seems to represent a promising solution. This review covers the main achievements in the use of doped ZnO NPs for nanomedicine applications. Sol-gel, as well as hydrothermal and combustion methods are largely employed to prepare ZnO NPs doped with rare earth and transition metal elements. For both dopant typologies, biomedical applications were demonstrated, such as enhanced antimicrobial activities and contrast imaging properties, along with an improved biocompatibility and stability of the colloidal ZnO NPs in biological media. The obtained results confirm that the doping of ZnO NPs represents a valuable tool to improve the corresponding biomedical properties with respect to the undoped counterpart, and also suggest that a new application of ZnO NPs in nanomedicine can be envisioned.
The Modified Helmholtz Equation on a Regular Hexagon—The Symmetric Dirichlet Problem
Authors: Kalimeris Konstantinos , Fokas Athanassios S.
Published: July 2020

Using the unified transform, also known as the Fokas method, we analyse the modified Helmholtz equation in the regular hexagon with symmetric Dirichlet boundary conditions; namely, the boundary value problem where the trace of the solution is given by the same function on each side of the hexagon. We show that if this function is odd, then this problem can be solved in closed form; numerical verification is also provided.
Novel Biomarkers of Mastitis in Goat Milk Revealed by MALDI-TOF-MS-Based Peptide Profiling
Authors: Matuozzo Monica , Spagnuolo Maria Stefania , Hussein Hany A. , Gomaa A. M. , Scaloni Andrea , D’Ambrosio Chiara
Published: July 2020

Mastitis is the most common infection of dairy goats impairing milk production and quality, which is usually recognized by mammary gland visual inspection and palpation. Subclinical forms of the disease are also widely represented, which lack the typical signs of the clinical ones but are still associated with reduced production and safety for human consumption of milk, generally presenting a high bacterial count. In order to obtain novel analytical tools for rapid and non-invasive diagnosis of mastitis in goats, we analyzed milk samples from healthy, subclinical and clinical mastitic animals with a MALDI-TOF-MS-based peptidomic platform, generating disease group-specific spectral profiles whose signal intensity and mass values were analyzed by statistics. Peculiar spectral signatures of mastitis with respect to the control were identified, while no significant spectral differences were observed between clinical and subclinical milk samples. Discriminant signals were assigned to specific peptides through nanoLC-ESI-Q-Orbitrap-MS/MS experiments. Some of these molecules were predicted to have an antimicrobial activity based on their strong similarity with homolog bioactive compounds from other mammals. Through the definition of a panel of peptide biomarkers, this study provides a very rapid and low-cost method to routinely detect mastitic milk samples even though no evident clinical signs in the mammary gland are observed.
Transient Administration of Dopaminergic Precursor Causes Inheritable Overfeeding Behavior in Young Drosophila melanogaster Adults
Authors: Moulin Thiago C. , Ferro Federico , Berkins Samuel , Hoyer Angela , Williams Michael J. , Schiöth Helgi B.
Published: July 2020

Imbalances in dopaminergic signaling during development have been indicated as part of the underlying neurobiology of several psychiatric illnesses, including schizophrenia, major depression, bipolar disorder, and food addiction. Yet, how transient manipulation of dopaminergic signaling influences long-lasting behavioral consequences, or if these modifications can induce inheritable traits, it is still not understood. In this study, we used the Drosophila melanogaster model to test if transient pharmacological activation of the dopaminergic system leads to modulations of feeding and locomotion in adult flies. We observed that transient administration of a dopaminergic precursor, levodopa, at 6 h, 3 days or 5 days post-eclosion, induced overfeeding behavior, while we did not find significant effects on locomotion. Moreover, this phenotype was inherited by the offspring of flies treated 6 h or 3 days post-eclosion, but not the offspring of those treated 5 days post-eclosion. These results indicate that transient alterations in dopaminergic signaling can produce behavioral alterations in adults, which can then be carried to descendants. These findings provide novel insights into the conditions in which environmental factors can produce transgenerational eating disorders.
Integrative Structural and Computational Biology of Phytases for the Animal Feed Industry
Authors: Nezhad Nima Ghahremani , Raja Abd Rahman Raja Noor Zaliha , Normi Yahaya M. , Oslan Siti Nurbaya , Shariff Fairolniza Mohd , Leow Thean Chor
Published: July 2020

Resistance to high temperature, acidic pH and proteolytic degradation during the pelleting process and in the digestive tract are important features of phytases as animal feed. The integration of insights from structural and in silico analyses into factors affecting thermostability, acid stability, proteolytic stability, catalytic efficiency and specific activity, as well as N-glycosylation, could improve the limitations of marginal stable biocatalysts with trade-offs between stability and activity. Synergistic mutations give additional benefits to single substitutions. Rigidifying the flexible loops or inter-molecular interactions by reinforcing non-bonded interactions or disulfide bonds, based on structural and roof mean square fluctuation (RMSF) analyses, are contributing factors to thermostability. Acid stability is normally achieved by targeting the vicinity residue at the active site or at the neighboring active site loop or the pocket edge adjacent to the active site. Extending the positively charged surface, altering protease cleavage sites and reducing the affinity of protease towards phytase are among the reported contributing factors to improving proteolytic stability. Remodeling the active site and removing steric hindrance could enhance phytase activity. N-glycosylation conferred improved thermostability, proteases degradation and pH activity. Hence, the integration of structural and computational biology paves the way to phytase tailoring to overcome the limitations of marginally stable phytases to be used in animal feeds.
On-Line Gaseous Formaldehyde Detection Based on a Closed-Microfluidic-Circuit Analysis
Authors: Becker Anaïs , Andrikopoulou Christina , Bernhardt Pierre , Trocquet Claire , Le Calvé Stéphane
Published: July 2020

This paper describes a compact microfluidic analytical device in a closed-circuit developed for the detection of low airborne formaldehyde levels. The detection is based on the passive trapping of gaseous formaldehyde through a microporous tube into the acetylacetone solution, the derivative reaction of formaldehyde with acetylacetone to form 3,5-Diacetyl-1,4-dihydrolutidine (DDL) and the detection of DDL by fluorescence. The recirculation mode of the analytical device means that the concentration measurement is carried out by quantification of the signal increase in the liquid mixture over time, the instantaneous signal increase rate being proportional to the surrounding gaseous formaldehyde concentration. The response of this novel microdevice is found to be linear in the range 0–278 µg m−3. The reagent volume needed is flexible and depends on the desired analytical resolution time and the concentration of gaseous formaldehyde in the environment. Indeed, if either the gaseous concentration of formaldehyde is high or the reagent volume is low, the fluorescence signal of this recirculating liquid solution will increase very rapidly. Consequently, the sensitivity simultaneously depends on both the reagent volume and the temporal resolution. Considering a reagent volume of 6 mL, the hourly and daily detection limits are 2 and 0.08 µg m−3, respectively, while the reagent autonomy is more than 4 days the airborne formaldehyde concentration does not exceed 50 µg m−3 as it is usually the case in domestic or public indoor environments.
Magnetron Sputtering of Nanolaminated Cr2AlB2
Authors: Berastegui Pedro , Riekehr Lars , Jansson Ulf
Published: July 2020

A ternary Cr2AlB2 phase was deposited as a film using magnetron sputtering. Its anisotropic structure displays both structural and chemical similarities with the nanolaminated MAX phases (Mn+1AXn (n = 1–3) where M usually is an early transition metal, A is typically an element in group 13–14 and X is C or N), and can be described as CrB slabs separated by layers of Al. Combinatorial sputtering was used to optimise the sputtering process parameters for films with the Cr2AlB2 composition. The influences of substrate, temperature and composition were studied using X-ray diffraction, X-ray photoelectron spectroscopy and electron microscopy. Films deposited at room temperature were X-ray amorphous but crystalline films could be deposited on MgO substrates at 680 °C using a composite Al-B, Cr and Al targets. X-ray diffraction analyses showed that the phase composition and texture of the films was strongly dependent on the chemical composition. Films with several phases or with a single Cr2AlB2 phase could be deposited, but an additional Al target was required to compensate for a loss of Al at the high deposition temperatures used in this study. The microstructure evolution during film growth was strongly dependent on composition, with a change in texture in Al-rich films from a preferred [010] orientation to a [100]/[001] orientation. A model based on Al desorption from the surface of the growing grains is proposed to explain the texture variations.
The Effect of Charged Ag Nanoparticles on Thin Film Growth during DC Magnetron Sputtering
Authors: Jang Gil-Su , Kim Du-Yun , Hwang Nong-Moon
Published: July 2020

The possibility that charged nanoparticles (CNPs) are generated in the gas phase during direct current (DC) magnetron sputtering of Ag is studied. Sputtered Ag particles could be captured on an ultrathin amorphous carbon membrane for transmission electron microscopy (TEM) observation. It is confirmed that the average particle size and the total area of deposition under the condition of the positive bias applied to the substrate are bigger than those under the condition of the negative bias applied to the substrate. The results indicate that some of the sputtered Ag particles are negatively charged. To evaluate the contribution of negatively-charged particles to the film growth, Ag thin films were deposited for 30 min on the Si substrate with the substrate biases of −300, 0 and +300 V and analyzed by field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and four-point probe. When +300 V was applied to the substrate, the film growth rate was highest with the film thickness of 85.0 nm, the crystallinity was best with the smallest full width at half maximum (FWHM) value of 0.44 and the resistivity was smallest with 3.67 μΩ·cm. In contrast, when −300 V was applied to the substrate, the film growth rate was lowest with the film thickness of 68.9 nm, the crystallinity was worst with the largest FWHM value of 0.53 and the resistivity was largest with 8.87 μΩ·cm. This result indicates that the charge plays an important role in film growth and can be a new process parameter in sputtering.
Low-Temperature Preparation of SiO2/Nb2O5/TiO2–SiO2 Broadband Antireflective Coating for the Visible via Acid-Catalyzed Sol–Gel Method
Authors: Xu Siyuan , Jia Hongbao , Wang Chunyang , Zhao Wenping , Wang Ying , Yang Chunming , Wu Henan , Zhu Jiang , Wang Biao , Wang Qian
Published: July 2020

Multilayer broadband antireflective (AR) coatings consisting of porous layers usually suffers poor functional durability. Based on a quarter-half-quarter multilayer structure, AR coatings with dense SiO2 film as the top layer are designed, and refractive index for each layer is optimized. After heat-treated at only 150 °C, refractive index of Nb2O5 film reaches to 2.072 (at 550 nm), which can meet design requirements of the middle layer. TiO2–SiO2 composites with controllable refractive indices are selected to be used as the bottom layer. The obtained triple-layer AR coating presents excellent performance, and the average transmittance at 400–800 nm attains 98.41%. Dense layers endow the multilayer structure good abrasion-resistance, and hexamethyldisilazane is further used to modify the surface of the AR coating, which can greatly improve the hydrophobicity of the coating. The proposed triple-layer broadband AR coating has potential value in practical applications of sol–gel deposition.
Thermomechanical Processing of AZ31-3Ca Alloy Prepared by Disintegrated Melt Deposition (DMD)
Authors: Rao Kamineni Pitcheswara , Suresh Kalidass , Prasad Yellapregada Venkata Rama Krishna , Gupta Manoj
Published: July 2020

Mg-3Zn-1Al (AZ31) alloy is a popular wrought alloy, and its mechanical properties could be further enhanced by the addition of calcium (Ca). The formation of stable secondary phase (Mg,Al)2Ca enhances the creep resistance at the expense of formability and, therefore, necessitates the establishment of safe working window(s) for producing wrought products. In this study, AZ31-3Ca alloy has been prepared by the disintegrated melt deposition (DMD) processing route, and its hot deformation mechanisms have been evaluated, and compared with similarly processed AZ31, AZ31-1Ca and AZ31-2Ca magnesium alloys. DMD processing has refined the grain size to 2–3 μm. A processing map has been developed for the temperature range 300–450 °C and strain rate range 0.0003–10 s−1. Three working domains are established in which dynamic recrystallization (DRX) readily occurs, although the underlying mechanisms of DRX differ from each other. The alloy exhibits flow instability at lower temperatures and higher strain rates, which manifests as adiabatic shear bands. A comparison of the processing maps of these alloys revealed that the hot deformation mechanisms have not changed significantly by the increase of Ca addition.
Long-Term Effects of Angiotensin Receptor–Neprilysin Inhibitors on Myocardial Function in Chronic Heart Failure Patients with Reduced Ejection Fraction
Authors: Poglajen Gregor , Anžič-Drofenik Ajda , Zemljič Gregor , Frljak Sabina , Cerar Andraž , Okrajšek Renata , Šebeštjen Miran , Vrtovec Bojan
Published: July 2020

Background. We sought to evaluate the long-term effects of angiotensin receptor blocker–neprilysin inhibitor (ARNI) therapy on reverse remodeling of the failing myocardium in HFrEF patients. Methods. We performed a prospective non-randomized longitudinal study on 228 HFrEF patients treated with ARNI at our center. Prior to ARNI introduction all patients received stable doses of ACEI/ARB for at least six months. Clinical, biochemical and echocardiography data were obtained at ARNI introduction and 12-month follow-up. Results At follow-up, we found significant improvements in LVEF (29.7% ± 8% vs. 36.5% ± 9%; p < 0.001), LVOT-VTI (14.8 ± 4.2 cm vs. 17.2 ± 4.2 cm; p < 0.001), TAPSE (1.7 ± 0.5 cm vs. 2.1 ± 0.6 cm; p < 0.001) and LV-EDD (6.5 ± 0.8 cm vs. 6.3 ± 0.9 cm; p = 0.001). NT-proBNP serum levels also decreased significantly (1324 (605, 3281) pg/mL vs. 792 (329, 2022) pg/mL; p = 0.001). A total of 102 (45%) of patients responded favorably to ARNI (ΔLVEF < +5%; Group A) and 126 (55%) patients achieved ΔLVEF ≥ +5% (Group B). The two groups differed significantly in age, heart failure etiology, baseline LVEF and baseline NT-proBNP. On multivariable analysis, nonischemic heart failure, LVEF < 30% and NT-proBNP < 1500 pg/mL emerged as independent correlates of favorable response to ARNI therapy. Conclusion. ARNI therapy appears to improve echocardiographic parameters of left and right ventricular function in HFrEF patients above the effect of pre-existing optimal medical management. These effects may be particularly pronounced in patients with nonischemic heart failure, LVEF < 30% and lower degree of neurohumoral activation.
Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia
Authors: Pinto Andreia L. , Rai Ranjit K. , Hogg Claire , Burgoyne Thomas
Published: July 2020

Primary ciliary dyskinesia (PCD) is a disorder that affects motile cilia in the airway that are required for the removal of mucus, debris, and pathogens. It is important to diagnose PCD in early childhood to preserve lung function. The confirmation of a diagnosis relies on the assessment of ciliary ultrastructure by transmission electron microscopy (TEM). TEM involves the quantitative assessment of the ciliary ultrastructure to identify PCD defects as well as abnormalities resulting from infection. Many specialist diagnostic centres still rely on physical counters to tally results and paper notes to summarise findings before transferring the results to computer databases/records. To speed up the diagnostic data collection and increase the protection of patient information, we have developed digital ciliary feature counters that conform to the PCD reporting international consensus guideline. These counters can be used on a computer or tablet, and automatically generate notes regarding sample observations. We show that the digital counters are easy to use and can generate TEM diagnostic reports that will be useful for many PCD diagnostic centres.
Effect of Temperature on Electromagnetic Performance of Active Phased Array Antenna
Authors: Wang Yan , Wang Congsi , Lian Peiyuan , Xue Song , Liu Jing , Gao Wei , Shi Yu , Wang Zhihai , Yu Kunpeng , Peng Xuelin , Du Biao , Xiao Song
Published: July 2020

Active phased array antennas (APAAs) can suffer from the effects of harsh thermal environments, which are caused by the large quantity of power generated by densely packed T/R modules and external thermal impacts. The situation may be worse in the case of limited room and severe thermal loads, due to heat radiation and a low temperature sink. The temperature field of the antenna can be changed. Since large numbers of temperature-sensitive electronic components exist in T/R modules, excitation current output can be significantly affected and the electromagnetic performance of APAAs can be seriously degraded. However, due to a lack of quantitative analysis, it is difficult to directly estimate the effect of temperature on the electromagnetic performance of APAAs. Therefore, this study investigated the electromagnetic performance of APAAs as affected by two key factors—the uniformly distributed temperature field and the temperature gradient field—based on different antenna shapes and sizes, to provide theoretical guidance for their thermal design.
AntsOMG: A Framework Aiming to Automate Creativity and Intelligent Behavior with a Showcase on Cantus Firmus Composition and Style Development
Authors: Chang Chun-Yien , Chen Ying-Ping
Published: July 2020

Creative behavior is one of the most fascinating areas in intelligence. The development of specific styles is the most characteristic feature of creative behavior. All important creators, such as Picasso and Beethoven, have their own distinctive styles that even non-professional art lovers can easily recognize. Hence, in the present work, attempting to achieve cantus firmus composition and style development as well as inspired by the behavior of natural ants and the mechanism of ant colony optimization (ACO), this paper firstly proposes a meta-framework, called ants on multiple graphs (AntsOMG), mainly for roughly modeling creation activities and then presents an implementation derived from AntsOMG for composing cantus firmi, one of the essential genres in music. Although the mechanism in ACO is adopted for simulating ant behavior, AntsOMG is not designed as an optimization framework. Implementations can be built upon AntsOMG in order to automate creation behavior and realize autonomous development on different subjects in various disciplines. In particular, an implementation for composing cantus firmi is shown in this paper as a demonstration. Ants walk on multiple graphs to form certain trails that are composed of the interaction among the graph topology, the cost on edges, and the concentration of pheromone. The resultant graphs with the distribution of pheromone can be interpreted as a representation of cantus firmus style developed autonomously. Our obtained results indicate that the proposal has an intriguing effect, because significantly different styles may be autonomously developed from an identical initial configuration in separate runs, and cantus firmi of a certain style can be created in batch simply by using the corresponding outcome. The contribution of this paper is twofold. First, the presented implementation is immediately applicable to the creation of cantus firmi and possibly other music genres with slight modifications. Second, AntsOMG, as a meta-framework, may be employed for other kinds of autonomous development with appropriate implementations.
Influence of the Size and Shape of Magnetic Core on Thermal Parameters of the Inductor
Authors: Detka Kalina , Górecki Krzysztof
Published: July 2020

In this paper, a new thermal model of the inductor is proposed. This model takes into account self-heating in the core and in the winding, and mutual thermal couplings between the mentioned components of the inductor. The form of the elaborated thermal model is presented. In this model, the influence of power dissipated in the core and in the winding of the inductor on the efficiency of heat removal is taken into account. Correctness of the model is verified experimentally for inductors containing ferrite cores of different shapes and dimensions. The good agreement between the results of calculations and measurements is obtained. On the basis of the obtained findings, the influence of volume and the shape of the core on thermal resistances and thermal capacitances occurring in this model is discussed.
Methods of Modulation for Current-Source Single-Phase Isolated Matrix Converter in a Grid-Connected Battery Application
Authors: Teck Chiang Goh , Sugiyama Takahide
Published: July 2020

This paper discusses three methods of modulation for a single-phase isolated matrix converter. The matrix converter is combined with a transformer integration to perform power decoupling control in order to reduce the number of component and capacitor volumes. Due to the reason of (i) Alternating current (AC/AC) direct conversion and (ii) transformer integration, obtaining a clean sinusoidal grid current waveform in the modulation of matrix converter (MC) is important. Three methods of modulation are compared in terms of control complexity, quality waveform, and inductive-capacitive-inductive (LCL) filter sizing. The principal control of each method is described. Finally, a prototype was tested to verify the validity and the effectiveness of grid current control and power decoupling in the spoken circuit structure.
Economic Comparison Between a Stand-Alone and a Grid Connected PV System vs. Grid Distance
Authors: Marino Concettina , Nucara Antonino , Panzera Maria Francesca , Pietrafesa Matilde , Pudano Alfredo
Published: July 2020

The limitation of fossil fuel uses and GHG (greenhouse gases) emissions reduction are two of the main objectives of the European energy policy and global agreements that aim to contain climate changes. To this end, the building sector, responsible for important energy consumption rates, requires a significant improvement of its energetic performance, an obtainable increase of its energy efficiency and the use of renewable sources. Within this framework, in this study, we analysed the economic feasibility of a stand-alone photovoltaic (PV) plant, dimensioned in two configurations with decreasing autonomy. Their Net Present Value at the end of their life span was compared with that of the same plant in both grid-connected and storage-on-grid configurations, as well as being compared with a grid connection without PV. The analysis confirms that currently, for short distances from the grid, the most suitable PV configuration is the grid-connected one, but also that the additional use of a battery with a limited capacity (storage on grid configuration) would provide interesting savings to the user, guaranteeing a fairly energetic autonomy. Stand-alone PV systems are only convenient for the analysed site from distances of the order of 5 km, and it is worth noting that such a configuration is neither energetically nor economically sustainable due to the necessary over-dimensioning of both its generators and batteries, which generates a surplus of energy production that cannot be used elsewhere and implies a dramatic cost increase and no corresponding benefits. The results have been tested for different latitudes, confirming what we found. A future drop of both batteries’ and PV generators’ prices would let the economic side of PV stand-alone systems be reconsidered, but not their energetic one, so that their use, allowing energy exchanges, results in being more appropriate for district networks. For all PV systems, avoided emissions of both local and GHG gases (CO2) have been estimated.
Moisture-Safe Cold Attics in Humid Climates of Europe and North America
Authors: Richter Jan , Staněk Kamil , Tywoniak Jan , Kopecký Pavel
Published: July 2020

During the last few decades, unheated attic spaces (so-called “cold attics”) have been one of the most moisture-problematic spaces in the building practices of Europe and North America. Problems with such spaces are usually associated with condensation, frost formation, and mould growth on the lower surfaces of the roof deck. To solve these problems, a number of qualitative studies were performed. Although these studies revealed many important pieces of information, some of them are seemingly not in accordance with one another. Therefore, there has not yet been presented cold attic design which is suitable (especially moisture-safe) for the whole target region. The aim of this paper is to determine whether such a design can be determined or state the reasons why it is not possible. Based on a review of published studies, 38 cold attic designs were selected for detailed analysis. Their comparative parameters were established, quantified, and compared. The moisture-safeness of each design was evaluated using an original approach, and the designs were sorted into groups based on their similarities. Despite many uncertainties, it was found that the criteria for moisture-safe cold attic design in the target region can likely be stated.
Slash Pile Burn Scar Restoration: Tradeoffs between Abundance of Non-Native and Native Species
Authors: Sexton Ian , Turk Philip , Ringer Lindsay , Brown Cynthia S.
Published: July 2020

The accumulation of live and dead trees and other vegetation in forests across the western United States is producing larger and more severe wildfires. To decrease wildfire severity and increase forest resilience, foresters regularly remove excess fuel by burning woody material in piles. This common practice could also cause persistent ecosystem changes such as the alteration of soil physical and chemical properties due to extreme soil heating, which can favor invasion by non-native plant species. The abundance and species richness of native plant communities may also remain depressed for many years after burning has removed vegetation and diminished propagules in the soil. This adds to the vulnerability of burned areas to the colonization and dominance by invasive species. Research into the use of revegetation techniques following pile burning to suppress invasion is limited. Studies conducted in various woodland types that investigated revegetation of pile burn scars have met with varying success. To assess the effectiveness of restoring pile burn scars in Rocky Mountain National Park, Colorado, we monitored vegetation in 26 scars, each about 5 m in diameter, the growing season after burning. Later that summer, we selected 14 scars for restoration that included soil scarification, seed addition, and pine duff mulch cover. We monitored the scars for four years, pre-restoration, and three years post-restoration and found that the cover of seeded species exceeded the surrounding unburned areas and unseeded controls. The restoration seeding suppressed cover of non-native species as well as native species that were not seeded during restoration. Our results suggest that restoration of pile burn scars could be a useful tool to retard the establishment of invasive plant species when there are pre-existing infestations near scars. However, this must be weighed against the simultaneous suppression of native species recruitment. Monitoring for periods more than three years will help us understand how long the suppression of native and non-native species by restoration species may persist.
Non-Financial Information Disclosures and Environmental Protection—Evidence from Romania and Greece
Authors: Artene Alin , Bunget Ovidiu-Constantin , Dumitrescu Alin-Constantin , Domil Aura-Emanuela , Bogdan Oana
Published: July 2020

Currently, sustainability and sustainable development are issues that concern society due to the high degree of pollution and the measures taken that, in many cases, do not prove their efficiency. Economic entities are aware that their involvement in the community has become necessary for the sustainable development of any business. The promotion of responsibility towards the environment is an important principle of corporate social responsibility (CSR), which is becoming an obligation that can no longer be exempted. Our intention in this paper was to study the changes generated by the European Union (EU) Directive 2014/95 regarding the manner in which oil entities listed at the Bucharest Stock Exchange (BSE) and the Athens Stock Exchange (ATHEX) between 2014 and 2018, report environment-related information. We also identified whether entities in fields that are known as polluting were more sensitive to environmental factors and if they tended to report more information compared to other business sectors. To achieve these objectives, we used qualitative and quantitative research. Namely, we analyzed the entities’ non-financial reports, to identify their social responsibility actions, and we tested if they were correlated with the environment protection and also with the 2014/95 European directive stipulations. For the entities operating in this polluting area, demonstrating transparency regarding the efforts and investments made to counteract the environmental impacts is important. Non-financial corporate reporting can be an opportunity to integrate sustainability into a company’s business practices and strategies to obtain benefits and to increase efficiency and, thus, to increase stakeholder confidence. The contribution of our study is to highlight the importance of non-financial information for the sustainable development of companies and the environment.
Importance of Soil, Stand, and Mycorrhizal Fungi in Abies balsamea Establishment in the Boreal Forest
Authors: Nagati Mélissande , Roy Mélanie , DesRochers Annie , Bergeron Yves , Gardes Monique
Published: July 2020

Research highlights: To understand differences in the establishment of balsam fir regeneration observed in the boreal forest, we examined how soil layer and microorganisms explained differences in growth and mycorrhization in three different stand types. Our experiment revealed positive and negative effects on growth of seedlings, and highlights the importance of biotic interactions in balsam fir establishment. Background and Objectives: In a context of climate change, understanding tree migration can be examined through changes in tree regeneration. At the ecotone between mixed and conifer boreal forest, regeneration of balsam fir northward is of particular interest because it thrives better under aspen-dominated stands as compared to adjacent spruce-dominated stands. As the understorey differs between these stands, with more Ericaceae under spruce and different ectomycorrhizal fungal communities in organic and mineral horizons, we hypothesized that biotic factors could explain differences in balsam fir establishment. Materials and Methods: Using a growth chamber experiment, we tested if differences in soil layers and modification of soil fungal communities would affect germination, mycorrhization, and growth of balsam fir seedlings in three different stand vegetation. We compared 12 treatments and followed 120 seedlings over three growth seasons. Results: We found similar survival in soils from aspen- and spruce-dominated stands, and a greater biomass on organic layers. In addition to this, a greater mycorrhization rate was found in aspen soils but improved germination in spruce soils. The presence of Ericaceae in spruce soils was associated with lower mycorrhization but did not affect other traits. Sterilization and therefore microorganisms affected mainly the number of ectomycorrhizae and the investment in root biomass. Finally, mycorrhization and biomass were correlated, but independent from N nutrition measured in needles. Conclusions: Our results highlighted the positive effects of organic soil layers and of mycorrhization on biomass, and showed that mycorrhization was increased under aspen as compared to other stand types. Our experiment also revealed positive effects of spruce soil on fir germination and showed that fir was able to grow and survive in all conditions. Our study suggests that fir establishment is affected by belowground multi-species interactions, and therefore highlights that biotic interactions shall be taken into account to understand and predict future tree migrations in the boreal forest.
Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis
Authors: Zhou Xiaofeng , Dai Yuqiu , Wu Haijun , Zhong Peiqiao , Luo Linjie , Shang Yangjuan , Tan Pengpeng , Peng Fangren , Tian Zhaoxia
Published: July 2020

Pecan (Carya illinoinensis) is a widely consumed edible woody oil species that is rich in unsaturated fatty acids (FAs) that are beneficial to human health. However, the genes and mechanisms regulating seed oil biosynthesis in pecan are not well understood. Here, we analyzed the expression patterns of genes involved in seed oil biosynthesis in two different varieties of pecan with distinct fruit maturation schedules and oil contents. We cloned the C. illinoinensis WRINKLED 1 (CiWRI1) gene, a homolog of ArabidopsisWRINKLED1 (AtWRI1), which plays a key role in FA synthesis. Overexpressing CiWRI1 restored lipid synthesis in the Arabidopsiswri1-1 mutant and rescued other phenotypic defects such as plant height, root length, and germination rate, suggesting that CiWRI1 is an ortholog of the AtWRI1 and is involved in the regulation of FA synthesis. To investigate the mechanism of CiWRI1 regulation, we cloned C. illinoinensis BIOTIN CARBOXYL CARRIER PROTEIN ISOFORM2 (CiBCCP2) and determined that the CiWRI1 protein directly binds to an ASML1/WRI1 (AW)-box motif in the CiBCCP2 gene promoter and thereby activates its transcription. CiBCCP2 overexpression partly rescued the phenotypic defects of the wri1-1 mutant, indicating that it is directly regulated by CiWRI1. Thus, de novo FA biosynthesis in seed is conserved across plant species; moreover, CiWRI1 regulates oil synthesis by directly controlling CiBCCP2 expression. These findings present novel potential targets for molecular-marker-assisted breeding of this commercially important plant.
Role of Global Self-Esteem in Predicting Life Satisfaction of Nursing Students in Poland, Spain and Slovakia
Authors: Kupcewicz Ewa , Grochans Elżbieta , Mikla Marzena , Kadučáková Helena , Jóźwik Marcin
Published: July 2020

Background: This study analyzed the role of global self-esteem and selected sociodemographic variables in predicting life satisfaction of nursing students in Poland, Spain and Slovakia. Methods: The study subjects were full-time nursing students from three European countries. A diagnostic survey was used as a research method, while the Rosenberg self-esteem scale (SES) and the satisfaction with life scale (SWLS) were used to collect data. Results: The research was performed on a group of 1002 students. The mean age of those surveyed was 21.6 (±3.4). The results showed significant differences both in the level of the global self-esteem index (F = 40.74; p < 0.0001) and in the level of general satisfaction with life (F = 12.71; p < 0.0001). A comparison of the structure of results demonstrated that there were significantly fewer students with high self-esteem in Spain (11.06%) than in Poland (48.27%) and in Slovakia (42.05%), while more students with a high sense of life satisfaction were recorded in Spain (64.90%) than in Poland (37.87%) or in Slovakia (47.44%). A positive, statistically significant correlation was found between global self-esteem and satisfaction with life in the group of Slovak students (r = 0.37; p < 0.0001), Polish students (r = 0.31; p < 0.0001) and Spanish students (r = 0.26; p < 0.0001). Furthermore, a regression analysis proved that three variables explaining a total of 12% output variation were the predictors of life satisfaction in Polish students. The regression factor was positive (ßeta = 0.31; R2 = 0.12), which indicates a positive correlation and the largest share was attributed to global self-esteem (9%). In the group of Spanish students, global self-esteem explained 7% (ßeta = 0.27; R2 = 0.07) of the output variation and 14% in the group of Slovak students (ßeta = 0.38; R2 = 0.14). Conclusions: The global self-esteem demonstrates the predictive power of life satisfaction of nursing students, most clearly marked in the group of Slovak students. The measurement of the variables under consideration may facilitate the planning and implementation of programs aimed at increasing self-esteem among young people and promoting the well-being of nursing students.
Effects of Weather on Coronavirus Pandemic
Authors: Bukhari Qasim , Massaro Joseph M. , D’Agostino Ralph B. , Khan Sheraz
Published: July 2020

The novel coronavirus (SARS-CoV-2) has spread globally and has been declared a pandemic by the World Health Organization. While influenza virus shows seasonality, it is unknown if COVID-19 has any weather-related affect. In this work, we analyze the patterns in local weather of all the regions affected by COVID-19 globally. Our results indicate that approximately 85% of the COVID-19 reported cases until 1 May 2020, making approximately 3 million reported cases (out of approximately 29 million tests performed) have occurred in regions with temperature between 3 and 17 °C and absolute humidity between 1 and 9 g/m3. Similarly, hot and humid regions outside these ranges have only reported around 15% or approximately 0.5 million cases (out of approximately 7 million tests performed). This suggests that weather might be playing a role in COVID-19 spread across the world. However, this role could be limited in US and European cities (above 45 N), as mean temperature and absolute humidity levels do not reach these ranges even during the peak summer months. For hot and humid countries, most of them have already been experiencing temperatures >35 °C and absolute humidity >9 g/m3 since the beginning of March, and therefore the effect of weather, however little it is, has already been accounted for in the COVID-19 spread in those regions, and they must take strict social distancing measures to stop the further spread of COVID-19. Our analysis showed that the effect of weather may have only resulted in comparatively slower spread of COVID-19, but not halted it. We found that cases in warm and humid countries have consistently increased, accounting for approximately 500,000 cases in regions with absolute humidity >9 g/m3, therefore effective public health interventions must be implemented to stop the spread of COVID-19. This also means that ‘summer’ would not alone stop the spread of COVID-19 in any part of the world.
Correlation of Mortality Burdens of Cerebrovascular Disease and Diabetes Mellitus with Domestic Consumption of Soya and Palm Oils
Authors: Ismail Maznah , Alsalahi Abdulsamad , Khaza’ai Huzwah , Imam Mustapha Umar , Ooi Der Jiun , Samsudin Mad Nasir , Idrus Zulkifli , Sokhini Muhammed Ha’iz Mohd , A. Aljaberi Musheer
Published: July 2020

Background: Cerebrovascular diseases (CBVDs) and diabetes mellitus (DM) are interrelated and cumbersome global health burdens. However, the relationship between edible oils consumption and mortality burdens of CBVDs and DM has not yet been evaluated. This review aims to explore correlations between per capita mortality burdens of CBVDs and DM, as well as food consumption of palm or soya oils in 11 randomly selected countries in 2005, 2010, and 2016. Methods: After obtaining data on food consumption of palm and soya oils and mortality burdens of CBVDs and DM, correlations between the consumption of oils and mortality burdens of diseases were explored. Results: There was a positive correlation between the consumption of soya oil with the mortality burden of CBVDs in Australia, Switzerland, and Indonesia, as well as the mortality burden of DM in the USA. The consumption of palm oil had a positive correlation with the mortality burden of DM in Jordan only. Conclusions: Food consumption of soya oil in several countries possibly contributes to the mortality burden of CBVDs or DM more than food consumption of palm oil, which could be a possible risk factor in the mortality burdens of CBVDs and DM.
The Impact of Environmental Social Media Publications on User Satisfaction with and Trust in Tourism Businesses
Authors: Martínez-Navalón Juan Gabriel , Gelashvili Vera , Saura José Ramón
Published: July 2020

The main aim of the present study was to analyze whether publications related to environmental sustainability in social media directly and positively influence user satisfaction with and trust in tourism businesses. Our second goal was to determine whether the influence of environmental sustainability and satisfaction is moderated by users’ gender. Data collection was performed using a questionnaire. The questionnaire responses were analyzed using the partial least squares-structural equation modeling (PLS-SEM) methodology. The results have shown that there is a positive relationship between environmental sustainability, satisfaction, and trust generated by tourism companies through their publications on social media, and that this relationship is not conditioned by users’ gender. The results of the present study contribute to the literature by bridging the gap in research on tourism enterprises and their strategies regarding social media publications. Our findings also provide important implications related to the content of environmental sustainability strategies and social media communication for tourism companies.
Implementation of IoT-Based Air Quality Monitoring System for Investigating Particulate Matter (PM10) in Subway Tunnels
Authors: Jo Jun Ho , Jo ByungWan , Kim Jung Hoon , Choi Ian
Published: July 2020

Air quality monitoring for subway tunnels in South Korea is a topic of great interest because more than 8 million passengers per day use the subway, which has a concentration of particulate matter (PM10) greater than that of above ground. In this paper, an Internet of Things (IoT)-based air quality monitoring system, consisting of an air quality measurement device called Smart-Air, an IoT gateway, and a cloud computing web server, is presented to monitor the concentration of PM10 in subway tunnels. The goal of the system is to efficiently monitor air quality at any time and from anywhere by combining IoT and cloud computing technologies. This system was successfully implemented in Incheon’s subway tunnels to investigate levels of PM10. The concentration of particulate matter was greatest between the morning and afternoon rush hours. In addition, the residence time of PM10 increased as the depth of the monitoring location increased. During the experimentation period, the South Korean government implemented an air quality management system. An analysis was performed to follow up after implementation and assess how the change improved conditions. Based on the experiments, the system was efficient and effective at monitoring particulate matter for improving air quality in subway tunnels.
The Meaning of Surviving Three Years after a Heart Transplant—A Transition from Uncertainty to Acceptance through Adaptation
Authors: Lindberg Catharina , Almgren Matilda , Lennerling Annette , Forsberg Anna
Published: July 2020

The rationale was to longitudinally follow-up interviews performed with heart recipients at their one-year examination in order to deepen the understanding of the meaning of surviving a heart transplant. The aim was to explore the meaning of surviving three years after a heart transplant compared to one year and to identify what constitutes the change process. A phenomenological–hermeneutic method was used. This multicenter study was carried out at the two hospitals in Sweden where heart transplants are performed. A total of 13 heart recipients who survived three years after a heart transplant were invited to participate in this three-year follow-up study and 12 accepted, 3 women and 9 men, with a mean age of 51.25 years. The naïve understanding revealed that the heart recipients strongly accepted their life situation and that time had enabled this acceptance of limitations through adaptation. The thematic structural analyses cover six themes illustrating the meaning of acceptance and adaptation, i.e., accepting life as it is, adapting to post-transplant limitations, adapting to a changed body, social adaptation, showing gratitude and trusting oneself and others. In conclusion, achieving acceptance and a solid sense of self-efficacy after heart transplantation is a time-consuming process that involves courage to face and accept the reality and adapt in every life dimension.
Adherence to Mediterranean Diet among Students from Primary and Middle School in the Province of Taranto, 2016–2018
Authors: Bonaccorsi Guglielmo , Furlan Federica , Scocuzza Marisa , Lorini Chiara
Published: July 2020

The Mediterranean diet represents one of the healthiest dietary patterns, but nowadays it is increasingly being ignored in schools and by families. The aim of this study is to assess the adherence to the Mediterranean diet by pupils living in a small Southern Italian municipality, and whether it is a predictor of nutritional status.The degree of adherence to the Mediterranean diet, the socio-economic status and the nutritional status of 314 students (6–14 years) were tested during the 2016/2017 and 2017/2018 school years with the help of a questionnaire comprising the Mediterranean Diet Quality Index for Children and Adolescents (KIDMED) test. Multivariate logistic regression analysis was used to assess the predictive role of the KIDMED score and the other variables with respect to nutritional status. Adherence to the Mediterranean diet is high, medium and poor in, respectively, 24.8, 56.4 and 18.8% of students; it varies depending on gender and age, with females and older students showing higher values. In the multivariate logistic regression model, sex and KIDMED level are become significant predictors of nutritional status. This study highlights the need for intervention in the form of school projects—also involving families—to promote healthier eating habits in younger generations.