bluuebunny/crossref_metadata_2025 huggingface dataset:
Benthic macrofaunal assemblage in seagrass-mangrove complex and adjacent ecosystems of Punang-Sari Estuary, Lawas, Sarawak, Malaysia
Authors: AL-ASIF ABDULLA , HAMLI HADI , Kamal Abu Hena Mustafa , IDRIS MOHD HANAFI , GERUSU GEOFFERY JAMES , ISMAIL JOHAN BIN , KARIM NURUL ULFAH
Published: November 2020

Abstract. Al-Asif A, Hamli H, Abu Hena MK, Idris MH, Gerusu GJ, Ismail JB, Karim NU. 2020. Benthic macrofaunal assemblage in seagrass-mangrove complex and adjacent ecosystems of Punang-Sari Estuary, Lawas, Sarawak, Malaysia. Biodiversitas 21: 4606-4615. Present study dealt with the on faunal and in faunal assemblage from seagrass bed, mangrove area, and adjacent non-mangrove and seagrass (NMS) in Punang-Sari estuary, Lawas, Sarawak, Malaysia. Samples were collected from June to July 2019 by using quadrates and handpicking from the outside the sampling area to know the real checklist of surface macrofauna in this area. A ten-meter transect line was settled in three habitat areas where sampling was performed by putting three quadrates (0.35m×0.35m). Epifauna and infauna samples were collected from inside the quadrate and sieved using 0.4 mm mesh size sieve. A total of 111 species of gastropod (87 species from 30 families), bivalve (18 species from 9 families), Polychaeta (2 species), echinoderms (1 species), and crustacean (3 species) were recorded during the study period. Seagrass meadows comprised most diverse and abundant faunal (50 species) assemblage followed by mangrove habitats (48 species), and NMS (20 species). The dendrogram revealed two significant habitats in the sampling site. PCA analysis revealed, seagrass habitat sheltered a higher number of species followed by mangrove and NMS area. Jaccard similarity index revealed seagrass and mangrove habitats (0.42) contains the highest similar species amongst all habitats compared to mangrove and non-seagrass-mangrove area (0.26). Out of total species recorded (101), only ten species were reported as least concern, and 2 species hold deficient data status, and the rest are not evaluated. Bio-assemblage in seagrass habitat was found rich compared to other habitats, which could be useful for future ecological investigation and marine ranching.
Maximum entropy modeling for the conservation of Hopea odorata in riparian forests, central Thailand
Authors: ASANOK LAMTHAI , KAMYO TORLARP , MAROD DOKRAK
Published: November 2020

Abstract. Asanok L, Kamyo T, Marod D. 2020. Maximum entropy modeling for the conservation of Hopea odorata in riparian forests, central Thailand. Biodiversitas 21: 4663-4670. Hopea odorata plays a dominant role in both ecologically and economically in Thailand. We analyzed potentially suitable areas for H. odorata in the riparian zone of the Chao Phraya River using the software MaxEnt. Modeling included 164 occurrence records along with 19 climate-related variables, slope, aspect, and elevation. Precipitation was the key climatic variable influencing the distribution of H. odorata. Riparian areas along the Chao Phraya that were predicted to be highly suitable for this species were located in the provinces of Nakhon Sawan, Ang Thong, Phra Nakhon Si Ayutthaya, Pathum Thani, Nonthaburi, and Bangkok. The ROC AUC score was 0.891, indicating that MaxEnt is an excellent tool for predicting suitable regions for the restoration or cultivation of commercially and ecologically valuable species such as H. odorata. Models such as what we have presented here can facilitate habitat conservation and sustainable resource use for rare and important plants.
Short Communication: Variations of morphology, anatomy, and metabolite profiles of Citrus reticulata Blanco cv. Tawangmangu grafts produced by shoot tip grafting using several rootstocks
Authors: Einstivina Nuryandani  , Susandarini Ratna , Ari Indrianto  , Tri Rini Nuringtyas  , Artnice Mega Fathima  , Siti Subandiyah
Published: November 2020

Abstract. Nuryandani E, Susandarini R, Indrianto A, Nuringtyas RT, Fathima AM, Subandiyah S. 2020. Short Communication: Variations of morphology, anatomy, and metabolite profiles of Citrus reticulata Blanco cv. Tawangmangu grafts produced by shoot tip grafting using several rootstocks. Biodiversitas 21: 4671-4676.  Cultivation of Citrus reticulata Blanco cv. Tawangmangu, one of leading mandarin cultivars in Indonesia, remains challenging due to the limitations of suitable rootstocks. The use of suitable rootstocks will greatly determine the success of grafting and affect the performance of the scion. This study aimed to evaluate the morphological, anatomical, and metabolic profiles of grafts between Tawangmangu and rootstocks from various species using shoot tip grafting. Observations of morphological, anatomical, and metabolite variation were carried out eight weeks after the beginning of the growth of the scions. The morphological characters were observed on leaves and stems of the scions. Anatomical data were obtained from the examination of longitudinal sections of grafts prepared using paraffin-embedded method. Analysis of metabolite profiles was performed using LC-MS. The results showed that for two of the three rootstocks used, C. ichangensis × C. reticulata cv. Austera (Japansche Citroen (JC)) and Citrus (Fortunella) japonica (Fortunella) scion growth was vigorous and resulted in a large number of leaves and long stems. Morphological variation was found in the leaf shape, as indicated by wider and wavy leaves of grafts with Fortunella, while narrower and straighter leaves were found in grafts with JC. The grafting onto the third rootstock, C. hystrix, induced dwarfing of the scions. Variation in the anatomical profiles was found in vascular vessels, and in exodermic and endodermic cell sizes. Differences in metabolic profiles of the grafts were found compared to scion and rootstocks. Results of this study provide an important contribution to the successful improvement of Tawangmangu.
Biology, morphology and damage of the lesser Coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam
Authors: Nguyen Ung , NGUYEN THI-HIEN , CHAU NGUYEN-QUOC-KHANH , LE VAN-VANG , TRAN VAN-HAI
Published: November 2020

Abstract. Nguyen HU, Nguyen TH, Chau NQK, Le VV, Tran VH. 2020. Biology, morphology and damage of the lesser coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam. Biodiversitas 21: 4686-4694. The lesser coconut weevil, Diocalandra frumenti, is an emerging pest of coconut trees in Vietnam. To help develop control options for D. frumenti, this study investigated its morphological and biological characteristics, and quantified damage levels on coconut trees. Results from the study showed that attack by D. frumenti on coconut trees is correlated with characteristic damage symptoms (e.g., oozing sap) on all maturity stages of coconut fruits throughout the year (24.7% infestation), with higher damage levels on young fruits (57.6%). Results also showed that infestation levels on trees (58.9%), coconut bunches (19.4%), and fruits (7.77%) varied greatly. Adults have four different morphologies, but genetic study showed that they are all one species. The life cycle from egg to adult averaged 167± 34.3 days. The average development time for eggs, larvae, female pupae, male pupae, male adults, and female adults was 5.62 ± 0.62, 142.3 ± 34.3, 9.79 ± 0.86, 10.2 ± 1.26, 81.5 ± 34.7, and 81.8 ± 37.2 days, respectively. In conclusion, D. frumenti is an important pest in coconut trees with significant fluctuations in the duration of its development stages, and variation in shape, color, and size of the eggs, larvae, pupae, and adults. They can attack any coconut orchard and have harmful impacts on coconut trees, coconut bunches, and fruits throughout the year.
Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers
Authors: Hartati Sri , MULIAWATI ENDANG S.
Published: November 2020

Abstract. Hartati S, Muliawati ES. 2020. Short Communication: Genetic variation of Coelogyne pandurata, C. rumphii and their hybrids based on RAPD markers. Biodiversitas 21: 4709-4713. One effort to increase the genetic diversity of orchids is by crossing. This research aims to assess the genetic variation of a hybrid orchid obtained by crossing Coelogyne pandurata and C. rumphii and their hybrids based on RAPD markers. In this research, both parents were analyzed in three replications, while the hybrid was done in 10 replications. The study was conducted by analyzing DNA bands using RAPD markers with six primers, i.e. OPA 02, OPA 07, OPA 13, OPB 12, OPB 17, and OPD 08. Identification of the parents and their F1 hybrids showed 95.83% polymorphic bands with 43 bands measuring 200-2100 bp. The parents of C. rumphii, C. pandurata, and their hybrids showed similarity range of 0.16-1.00. The crossing of C. rumphii and C. pandurata resulted in a similarity of 0.5, shown in two large clusters. The first cluster consisted only of C. pandurata males and the second cluster consisted of C. rumphii females, together with all hybrid individuals. This study succeeded in creating new hybrids of orchids that have different characters from their parents, having a genetic variation of 23%.
Short Communication: The physical and chemical properties of nipah (Nypa fructicans) frond as an alternative feed for ruminants in Indonesia
Authors: Afdal Muhammad , Kaswari Teja , Fakhri Saitul , Suryani Heni
Published: November 2020

Abstract. Afdal M, Kaswari T, Fakhri S, Suryani H. 2020. Short Communication: The physical and chemical properties of nipah (Nypa fructicans) frond as an alternative feed for ruminants in Indonesia. Biodiversitas 21: 4714-4718. The aim of this study was to evaluate the physical and chemical properties, as well as the degradation of dry matter (DM) and organic matter (OM), of nipah (Nypa fructicans Wurmb.) frond, in view of its potency as an alternative source of feed. Samples of nipah frond were collected from the Sub-district of Geragai and Nipah Panjang, Indonesia, representing lowland brackish and coastal areas, respectively. All samples were explored to reveal their physical and chemical properties, degradation of DM and OM, and total gas production. The t test was applied to compare parameters between the Geragai and Nipah Panjang areas. There were no significant differences (P > 0.05) in frond weight and leaf weight between the two sample locations. The length of frond and the length from base to first leaf of nipah was significantly different (P < 0.05) between both locations. The number of leaves in one frond and the number of fruit in one bunch were highly significant different (P < 0.01) between the two sample regions. The DM and OM degradation of nipah fronds and leaves were not significantly different (P > 0.05). between Geragai and Nipah Panjang, while the DM and OM degradation of nipah leaves were also not significantly different (P > 0.05). However, total gas production from the leaves and fronds were highly significantly different (P < 0.01) between Geragai and Nipah Panjang. It can be summarized that the physical properties and chemical composition of the fronds and leaves of nipah in these areas are similar except for the length between base and the first leaf, the number of leaves, and the number of fruit in a single bunch, which were different. In terms of DM and OM degradation, there were no differences between the lowland brackish area and the coastal area, except for in terms of total gas production, which was significantly different between the two sample locations.
The production function and profitability analysis of Gracilaria sp. seaweed polyculture with milkfish (Chanos chanos) and black tiger shrimp (Penaeus monodon)
Authors: Diatin Iis , Effendi Irzal , Alvina Taufik Meri
Published: November 2020

Abstract. Diatin I, Effendi I, Taufik MA. 2020. The production function and profitability analysis of Gracilaria sp. seaweed polyculture with milkfish (Chanos chanos) and black tiger shrimp (Penaeus monodon). Biodiversitas 21: 4747-4754. Gracilaria seaweed is the main ingredient for making agar as a source of food and industry. Gracilaria culture in ponds with a polyculture system is one of the potential seaweed cultivation in Indonesia. This study was aimed to analyze the productivity, production input optimation, and profitability on the polyculture system of Gracilaria sp. with milkfish (Chanos chanos) and black tiger shrimp (Penaeus monodon). The survey method was used to notify the seaweed farmers from Indramayu, West Java, Indonesia. The study data were obtained from all seaweed polyculture farmers populations in the observational location by census containing two farmers groups, namely Gracilaria sp. with milkfish and black tiger shrimp (GMS symbol) and Gracilaria sp. with milkfish (GM symbol) polyculture system. This study resulted that GMS polyculture system obtained higher productivity than GM polyculture system. The GMS business scale was in the increasing return to scale condition, while GM was in the decreasing return to scale condition. The optimal condition of GMS polyculture system was performed by improving the black tiger shrimp seed input, milkfish seed, and milkfish feed, besides declining the working hours, while GM polyculture system was performed by decreasing the milkfish feed and farmers working hours. When the optimal condition is applied to the GMS polyculture system, then the business profit reaches five times, resulting in an increased profit margin of 112% with R/C becomes 134%. Meanwhile, GM polyculture system only reaches 65% business profit, 65% profit margin, and 25% R/C. The investment payback period from both polyculture systems became shorter. The application of production input efficiency on the GMS polyculture system is more prospective than GM polyculture system.
Thermostability, photostability, and toxicity of clove oil nanoparticles against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae)
Authors: Ikawati Silvi , Himawan Toto , Abadi Abdul Latief , Tarno Hagus
Published: November 2020

Abstract. Ikawati S, Himawan T, Abadi AL, Tarno H. 2020. Thermostability, photostability, and toxicity of clove oil nanoparticles against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae). Biodiversitas 21: 4764-4771. Problems related to the essential oils as insecticides are their volatility, poor solubility in water and environmental degradation (such as caused by high temperatures and UV light) which can adversely affect the application. An ideal botanical insecticide formulation would maintain the level of the main compounds adequate for pest control. This study was to evaluate the stability of polyethylene glycol (PEG) coated nanoparticles loaded with clove (Syzygium aromaticum) essential oil to high temperature and UV light also their toxicity to C. ferrugineus. There is a reduction in nanoparticle size after heating from 257 to 173 nm and there is no difference in size distribution. After heating and UV-light treatment, eugenol content in free clove oil decreased significantly, while on nanoparticles there were not significantly changed, that indicated greater stability on the nanoformulation. The median lethal concentration (LC50) of CO-NPs to C. ferrugineus adults value for 72 and 120 hours was 0.734 and 0.676%.
Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
Authors: YANI AHMAD , AMIN MOHAMAD , Fatchur Rohman  , SUARSINI ENDANG , PUTRA WIRA EKA
Published: November 2020

Abstract. Yani A, Amin M, Rohman F, Suarsini E, Putra WE. 2020. Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents. Biodiversitas 21: 4778-4786. The pollution of heavy metals by anthropogenic activities in freshwater, especially in lakes, reduces the quality and endangers the existence of aquatic flora and fauna. This happens in Lake Tempe, which is located, in South Sulawesi, Indonesia. The study aimed to evaluate the content of heavy metals, isolation, and identify a lead reduction of indigenous bacteria as candidates for bioremediation agents. It began by examining the level of cadmium (Cd), copper (Cu), and lead (Pb) in seven sampling sites of Tempe Lake. Furthermore, propagation and isolation, morphological observation, laboratory-scale reduction tests, and species identification of potential bacteria reduction Pb were performed by using 16s rDNA. The reduction test to Pb was measured using Atomic Absorption Spectrometry (AAS), and the research data were analyzed using One-Way ANOVA with the Duncan advanced method. The results showed that the Cd, Cu, and Pb content of the seven sampling points exceeded the water quality standard Following the Indonesian Government Regulation (IGR) and WHO standards. Pb has the highest concentration among the other heavy metals in the water sample reaching 0.40 mg/L. Four bacterial isolates could grow on Pb-enriched media (isolates A, B, C, and D). The Bacillus form was found in two different species bacteria (isolate A and D), while the cocci shape was found in two other species (isolate B and C). Those all Gram-negative bacteria were observed to be a reduction to Pb exposure within a bacterial medium. Interestingly, these indigenous bacteria could decrease Pb concentration ranging from 7.45 to 3.48 ppm for seven days. Furthermore, the sequencing data showed that the indigenous bacteria (isolate D) had a 99.90% similarity to the Comamonas testosteroni strain S-2 constructed using Neighbor-joining phylogenetic analysis. Therefore, the indigenous bacteria C. testosteroni strain S-2 from Tempe lake may be proposed as a bioremediation agent to reduce Pb the water contaminants.
The application of novel methods of Animal Barrier Screen and Kelambu Trap for mosquitoe’s surveillance in South and West Sulawesi, Indonesia
Authors: Rahma Nur , Hasan Hajar , Ratnasari Arini , Wahid Isra
Published: November 2020

Abstract. Rahma N, Hasan H, Ratnasari A, Wahid I. 2020. The application of novel methods of Animal Barrier Screen and Kelambu Trap for mosquitoe’s surveillance in South and West Sulawesi, Indonesia. Biodiversitas 21: 4787-4794. Mosquito’s surveillance requires effective protocols to catch mosquitoes in a large number of species and individuals while safe for humans. The effectiveness of two novel trap methods was compared during dry and wet seasons in rural (Maros), semi-urban (North Toraja) both in South Sulawesi Province, and rural with coastal areas (Pasangkayu) in West Sulawesi Province. Animal Barrier Screen (ABS) is a barrier screen placed near livestock, while Kelambu Trap (KT) is an innovative form of mosquito net. Both trap innovations showed effective in catching mosquitoes, but ABS was more effective in trapping the mosquitos (8,589 individuals) than KT (8,350 individuals). In contrast, the species caught were more diverse in KT (43 species, nine genera) than that in ABS (36 species, seven genera). During the wet season, ABS and KT caught 4,848 individuals (27 species, five genera) and 4,749 individuals (36 species, eight species), respectively. Mosquitoes were the most abundant in Northern Toraja (6,338 individuals), followed by in Maros (5,566 individuals) then Pasangkayu (5,035 individuals), but mosquito diversity was the highest in Pasangkayu (nine genera, 33 species) compared to that in Northern Toraja (six genera, 27 species) and Maros (five genera, 25 species). Based on the effectiveness of this trap, innovative insecticide can be added into ABS and KT traps then placed in the field. The number of mosquitoes can be significantly reduced so that it can also lower the potential spread of the mosquitoes-born viruses.
Insect diversity in various distances to forest edge in small nature reserve: A case study of Bantarbolang Nature Reserve, Central Java, Indonesia
Authors: Darsono Darsono , RIWIDIHARSO EDY , SANTOSO SLAMET , SUDIANA EMING , Yani EDY , NASUTION ERIE KOLLYA , APRILLIANA HEXA , CHASANAH TITI
Published: November 2020

Abstract. Darsono, Riwidiharso E, Santoso S, Sudiana E, Yani E, Nasution EK, Aprilliana H, Chasanah T. 2020. Insect diversity in various distances to forest edge in small nature reserve: A case study of Bantarbolang Nature Reserve, Central Java, Indonesia. Biodiversitas 21: 4821-4828. A nature reserve is a conservation area with the objective of conserving the biodiversity of the flora and fauna within its boundaries. However, many nature reserves are fragmented into small extent and isolated from natural habitat, causing the so-called edge effect. This research aimed to investigate insect diversity across various distances to forest edge in small and isolated protected areas. Research was conducted in the Bantarbolang Nature Reserve, Pemalang District, Central Java, Indonesia. A survey was conducted to determine the diversity of insects, especially those from the Diptera (flies), Lepidoptera (butterfly), and Hymenoptera (bees and wasp) orders. The environmental parameters recorded were temperature, humidity, and flowering plant diversity and abundance. Line transects were laid at a length of 100 m, parallel to the forest edge at three different distance ranges from the edge: 0-50 m, 50-100 m, and 100-150 m. Species diversity was measured using the Shannon-Wiener index, evenness index, and Sorensen similarity index. Over the 4-month study period, 1713 individual insects from 63 species and from the following three orders were found: Lepidoptera (Rhopalocera; 33 species, 5 families, 932 individuals); Hymenoptera (20 species, 423 individuals) and Diptera (10 species, 7 families, 376 individuals). The highest diversity was found at the edge of the forest and the lowest at the distance of 100-150 m from the forest edge. While the study area can support a diversity of insects, especially pollinating insects, but it is unable to support the conservation of light-sensitive butterflies. The results of this study suggest that small nature reserve can support a diversity of pollinating insects, especially from the Diptera, Lepidoptera, and Hymenoptera. However, it has limited conservation potential because of the significant impact of forest edges on species composition, especially on specialist butterfly species with habitat in forest interior.
The tolerance of oil palm (Elaeis guineensis) seedlings to Al stress is enhanced by citric acid and natural peat water
Authors: Hidayah Agus Nur , Yahya Sudirman , Sopandie Didy
Published: November 2020

Abstract. Hidayah AN, Yahya S, Sopandie D. 2020. The tolerance of oil palm (Elaeis guineensis) seedlings to Al stress is enhanced by citric acid and natural peat water. Biodiversitas 21: 4850-4858. Management technology on soil containing high levels of Aluminum (Al) toxicity is still needed to be developed so that the growth and development of plants will be optimum. The aims of the research were to investigate the response of oil palm seedlings (Elaeis guineensis Jacq.) toward aluminum stress, and to evaluate the effects of several exogenous compounds to improve the tolerance of oil palm. The research was conducted from September 2018 to March 2019 at PT Gunung Sejahtera Ibu Pertiwi, Central Kalimantan. This research consisted of two nutrient culture experiments, namely: Al toxicity on oil palms seedlings and the role of various exogenous compounds to improve plant tolerances. The results revealed that the solution at concentrations of 400 μM, 800 μM, and 1600 μM of Al significantly inhibited root growth, increased MDA levels, decreased the photosynthesis rate, activity of CAT and APX. Therefore, a solution at concentration of 400 μM of Al can be used as the selection level of Al tolerant oil palm varieties on nutrient culture. Ethephon at concentrations of 25 ppm, 50 ppm, and 100 ppm inhibited root and shoot growth, increased MDA levels but reduced the photosynthesis rate, chlorophyll content, APX, and CAT activity. Addition of 25 ppm and 50 ppm of citric acid, 200 ppm and 300 ppm of peat water significantly increased root length, root dry weight, photosynthesis rate, chlorophyll content, carotenoids, CAT, and APX activities as MDA levels decreased. Addition of citric acid and peat water enabled seedlings of oil palm to improve their tolerance to Al stress on nutrient culture.
Wild edible plants in four Agni tribes of Central-east and Northeast of Côte d’Ivoire: a comparative study
Authors: Malan Djah François , LITTA AMANI LÉOPOLD , KOUGBO MÉNÉKÉ DISTEL , DIOP AMADOU LAMINE , KOUASSI KOUASSI GÉRARD
Published: November 2020

Abstract. Malan DF, Litta AL, Kougbo MD, Diop AL, Kouassi KG. 2020. Wild edible plants in four Agni tribes of Central-east and Northeast of Côte d’Ivoire: a comparative study. Biodiversitas 21: 4896-4902. An ethnobotany survey was carried out in twelve villages of the four Agni tribes from the Center-east to Northeast of the Côte d'Ivoire, in order to assess the evolution of the knowledge and know-how of these peoples concerning wild edible plants. Frequency of citations and the Smith index of each of edible plants were obtained with data from the survey of 571 informants (279 men and 292 women). Factorial analysis of the correspondences was also performed in order to reveal the similarities between the different tribes. In total, 77 plant species (36 families and 67 genera) were cited as consumed by humans. The most used parts are the fruits (65.38%), and leaves (24.36%). Analysis of the similarities shows that only 18.82% of these species are common to the four tribes. In addition, knowledge related to wild edible plants is gradually degrading, for several reasons, including obsolescence of use and the unavailability of plants due to environmental degradation. Thus, the valuation and protection of these resources would be beneficial to populations.
Documentation of ritual plants used among the Aceh tribe in Peureulak, East Aceh District, Indonesia
Authors: Sutrisno Imam Hadi , AKOB BACHTIAR , NAVIA ZIDNI ILMAN , NURAINI NURAINI , SUWARDI ADI BEJO
Published: November 2020

Abstract. Sutrisno IH, Akob B, Navia ZI, Nuraini, Suwardi AB. 2020. Documentation of ritual plants used among the Aceh tribe in Peureulak sub-district, East Aceh, Indonesia. Biodiversitas 21: 4990-4998. Traditional ceremonies as part of human cultural products at a practical level cannot be separated from the use of natural resources, mainly plants. Concerning the use of plants in traditional ceremonies, the knowledge and use of plants by the community are decreasing. This study aims to document the ritual plants used by the Aceh tribe in the East Aceh district of Indonesia. The study was conducted in Peureulak sub-districts, East Aceh district, Indonesia. A field survey was involved 125 respondents were selected using random sampling. The interview used a questionnaire including plant species, vernacular names, uses, parts use, and ritual tradition. A total of 51 ritual plant species consisting of 47 genera and 34 families was used as ritual ceremonies in the study area. Ninety-two percent were cultivated and 8% were found to be growing wild. Most of the plants used for wedding ceremonies (18 species), followed by birth ceremonies (8 species), funeral ceremonies (5 species), and pregnancy ceremony (3 species). Socio-cultural aspects can be considered as being used for the conservation of ritual plants in the East Aceh region.
Root colonization by arbuscular mycorrhizal fungi (AMF) in various age classes of revegetation post-coal mine
Authors: Salim Mohammad Agus , BUDI R. SRI WILARSO , SETYANINGSIH LULUK , ISKANDAR ISKANDAR , WAHYUDI IMAM , KIRMI HIFZIL
Published: November 2020

Abstract. Salim MA, Budi RSW, Setyaningsih L, Iskandar, Wahyudi I, Kirmi H. 2020. Root colonization by arbuscular mycorrhizal fungi (AMF) in various age classes of revegetation post-coal mine. Biodiversitas 21: 5013-5022. This study aims to evaluate the status of colonization of the roots of the host plant in various age classes of revegetation of post-coal mining land associated with AMF spores populations and soil fertility. The study was conducted in the post-mining revegetation land at PT. Berau Coal, East Kalimantan. Isolation of the spores was carried out using the wet-sieving and centrifugation method, while the colonization of the roots was carried out by the root coloring technique. Revegetation activities have been able to increase the content of some nutrients in the soil. The AMF population shows tendency an increase along with the increasing revegetation age classes. Eight-year revegetation age classes have the highest average number of spores. Plant species and understorey have shown the existence of AMF colonization with different values for each species. AMF root colonization has a negative relationship with the number of spores in the soil. The C-organic, N-total, CEC Ca, Mg and Fe contents have a positive correlation with AMF root colonization, while available P, total P, K, and Al have a negative correlation with AMF root colonization. Mg was significantly correlated (r = 0.861) to AMF root colonization.
The Euphorbiaceae (Spurge Family) in Bogor Botanic Gardens, Indonesia: Diversity, conservation and utilization
Authors: Munawaroh Esti , YUZAMMI YUZAMMI , PURWANTO Y.
Published: November 2020

Abstract. Munawaroh E, Yuzammi, Purwanto Y. 2020. The Euphorbiaceae (Spurge Family) in Bogor Botanic Gardens, Indonesia: Diversity, conservation and utilization. Biodiversitas 21: 5021-5031. Euphorbiaceae (Spurge family) is the fourth largest family world-wide, consists of 1,354 species and 91 genera. The family is also a part of the Bogor Botanic Gardens collections. The Gardens has evolved to fulfill its mission through five principal functions: conservation, research, education, tourism, and environmental services. The aims of this study are to reveal the diversity, conservation, and its potential in the Bogor Botanic Gardens. This research was based on direct observation of all members of Euphorbiaceae family in the Gardens, as well as Registration Unit and the Garden’s catalog which were observed for more than 10 years. The Gardens collected 39 genera, 71 species, and 136 specimens where 91 specimens are trees, 38 specimens are shrubs, three specimens are climbers and four specimens are succulents. Most of the collections are native to Indonesia 75.91% (103 specimens) and only 33 specimens (24.09%) from overseas. Amongst native collections have dominated from Jawa 25.54% (34 specimens), Sumatra 24.08% (33 specimens). The others are from Sulawesi 9.48% (13 specimens), Kalimantan 7.29% (10 specimens), Maluku 6.56% (9 specimens), Papua 2.28% (three specimens), and Nusa Tenggara Timur 0.72% (one specimen). Many species of the family have potentially developed as traditional medicinal plants (11 species), ornamental plants (16 species), spices and vegetable (two species), timbers (14 species), rubber plants (one species), source of energy (two species) and toxic (six species). Several old collections have retained at the Gardens that aged over 75 years, such as Mallotus philippensis (Lam.) Mull. Arg. forma mollis, Acalypha wilkesiana Mull. Arg., Acalypha hispida Burm. var. sanderi (N.E.Br.) J. J. Sm., Acalypha wilkesiana Mull. Arg. forma Montana, Sapium aucuparium Jacq. and Ricinodendron heudelotii (Bail.) Heckel. A map of planting point of the Euphorbiaceae collections is provided.
Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
Authors: Sukma Dewi , Handini Aline Sisi , Sudarsono Sudarsono
Published: November 2020

Abstract. Sukma D, Handini AS, Sudarsono. 2020. Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids. Biodiversitas 21: 5054-5064. Chalcone synthase (CHS) is a key enzyme in flavonoid biosynthesis. The research aims to isolate and characterize the CHS gene nucleotide sequence diversity of nine Phalaenopsis and one Doritaenopsis genotypes. Genomic DNA was isolated from young leaves and used to PCR amplify the gene using CHS specific degenerate primers. Results of PCR amplification yielded DNA fragments of 700 bp. Upon sequencing and nucleotide sequence analysis, we found that the genomic fragments were partial CHS gene from Phalaenopsis and Doritaenopsis genotypes and deposited the sequences in the NCBI Genebank Database accession numbers of KR184089-KR184098. More analysis of the sequences confirmed they shared ranged from 81-99% sequence identities to known CHS genes from Phalaenopsis deposited in the NCBI Database. They also shared 84-100% amino acid residues identity to CHS polypeptides. Multiple sequence alignment (MSA) and phylogenetic analysis further revealed the determined CHS nucleotide sequences from Phalaenopsis and Doritaenopsis genotypes were closely related to CHS genes from other orchid species.
Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia
Authors: Wahyu Irawati , PINONTOAN REINHARD , YUWONO TRIWIBOWO
Published: November 2020

Abstract. Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21: 5077-5084. Biological wastewater treatment using activated sludge is a promising wastewater treatment solution for removing heavy metals. To improve the effectiveness of biological wastewater treatment, activated sludge must consist of bacteria that can remove heavy metals through the process of bioaccumulation and biosorption. This study was aimed to isolate indigenous copper resistant bacteria and determining their resistance to copper, as well as analyzing their ability to accumulate and remove copper. Copper resistant bacteria were isolated from activated sludge of water treatment plant in industrial plant in Rungkut, Surabaya. Resistance to copper was analyzed by determining the value of minimum inhibitory concentration (MIC). The ability of bacterial isolates to remove copper was analyzed by atomic absorption spectrophotometer. A total of six highly copper resistant bacteria were isolated and designated as B6.1, C8.1, C9.3, C9.4, C9.5, C10.4 isolates. All isolates were categorized as high resistant bacteria with the MICs of 9-11 mM CuSO4. The two highest copper resistant bacteria were isolates C10.4 and C9.4. The ability of the two isolates to accumulate copper was 8.02 mg and 4.83 mg per gram dry weight of cells and to remove of copper up to 20.45% and 17.66%, respectively.
Effects of ship-breaking activities on the abundance and diversity of macrobenthos in Sitakundu Coast, Bangladesh
Authors: Hossain Mohammad Belal , NOMAN MD. ABU , LIPI JAHANARA AKHTER , KAMAL ABU HENA MUSTAFA , IDRIS MOHD HANAFI
Published: November 2020

Abstract. Lipi JA, Noman MDA, Hossain MB, Abu Hena MK, Idris MH. 2020. Effects of ship-breaking activities on the abundance and diversity of macrobenthos in Sitakundu Coast, Bangladesh. Biodiversitas 21: 5085-5093. To articulate the ecological processes or anthropogenic impacts, it is necessary to explore various distributional patterns of benthic communities. The study was conducted to investigate the variability of macrobenthos between a ship braking and non-ship-breaking area along the Sitakunda coast, Chittagong, Bangladesh. This is the first comprehensive study that addresses the effects of ship-breaking activities on the variability of benthic communities in the study area. Macrobenthos were sampled from two different regions namely Bhatiary (ship-breaking area) and Banshbaria (non-ship-breaking area/reference area) in two different time periods (April and July) for comparative analyses. During the study period, Polychaeta was the most dominant group comprising 60% of the total macrobenthos all over the study area. Macrobenthos abundance and diversity demonstrated strong spatial variability. The mean abundance varied from 3799.75±3452.28 ind./m2 to 4107.25±2743.6 ind./m2 from the ship-breaking to non-ship-breaking area. Similar to macrobenthos abundance, the diversity index (H′) (varied between 2.45 and 1.85), species richness (D) (varied between 1.99 and 1.04), and the number of taxa (varied between 17.25 and 9.25) were higher in the non-ship-breaking area compared to the ship-breaking area. Multivariate analyses, nMDS, and CCA plot showed a distinct grouping for different location. Besides, the diversity indices of the Polychaeta community, and the presence or absence of several pollution indicator taxa revealed that Bhatiary (the ship-breaking area) was confronting deleterious effects of ship-breaking activities.
New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
Authors: Herlinda Siti , Efendi Riski Anwar , Suharjo Radix , Hasbi Hasbi , Setiawan Arum , Elfita Elfita , Verawaty Marieska
Published: November 2020

Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was carried out to confirm identity of Beauveria bassiana and Metarhizium anisopliae using sequence analysis of Internal Transcribed Spacer (ITS) region as well as the two unknown isolates of EPF and to investigate their filtrate and conidial insecticidal activity against S. litura larvae. The EPF species were identified based on morphological characters and molecular analysis. Their culture filtrate and conidial bioassays were performed on S. litura larvae. The species found were Penicillium citrinum (BKbTp), Talaromyces diversus (MSwTp1), B. bassiana (BSwTd4), and M. anisopliae (MSwTp3) and have been deposited in the GenBank. All EPF species have high insecticidal activity against S. litura larvae. The highest larvae mortality caused by filtrate was found on P. citrinum (98.67%) and not significantly different from those of T. diversus (96%), but significantly different from B. bassiana (86.67%) and M anisopliae (82.67%). The highest larvae mortality caused by the conidia was also occurred to P. citrinum (98.67%) which was not significantly different from those of T. diversus (97.33%), B. bassiana (86.67%), and M. anisopliae (86.67%). The LT50 caused by T. diversus filtrate was the shortest (6.19 days) and not significantly different from those caused by P. citrinum (6.62 days). The shortest LT50 caused by conidia was found on P. citrinum (5.45 days). Therefore, the conidia and filtrate of P. citrinum, a new emerging entomopathogenic fungus, has the highest insecticidal activity. P. citrinum has the potential to be developed into commercial mycoinsecticides.
Phenotypic diversity characterization of Kalang and Thale Noi Buffalo (Bubalus bubalis) in Indonesia and Thailand: Perspectives for the buffalo breeding development
Authors: Suhardi SUHARDI , SUMMPUNN PIJUG , DUANGJINDA MONCHAI , WUTHISUTHIMETHAVEE SUWIT
Published: November 2020

Abstract. Suhardi, Summpunn P, Duangjinda M, Wuthisuthimethavee S. 2020. Phenotypic diversity characterization of Kalang and Thale Noi Buffalo (Bubalus bubalis) in Indonesia and Thailand: Perspectives for the buffalo breeding development. Biodiversitas 21: 5128-5137. Kalang (KBuf) and Thale Noi buffaloes (TBuf) are swamp buffalo breeds important for biodiversity resources and permeate culture, religion, meat supply, and livelihood in Indonesia and Thailand. This study aimed to analyze the phenotypic characterization within breeds of KBuf/TBuf using quantitative and qualitative performance to define diversity. A total of 240 buffaloes from North (NK), East (EK), South Kalimantan (SK), Indonesia, and Phatthalung (PT), Thailand were observed. Phenotypic characterization showed female TBuf had the highest quantitative variable values with significant difference (P<0.05) from KBuf. Also, SK KBuf males had the highest qualitative variable values with significant difference (P<0.05) from EK and NK. The morphometric indices showed male buffaloes from SK and PT had close index values (P>0.05) and females from EK, SK, and PT showed a close relationship (P>0.05). The cumulative index results demonstrated KBuf/TBuf as very good meat-producing livestock. In conclusion, variations in qualitative and quantitative variables indicate phenotypic diversity in KBuf/TBuf which is a basis to perform selection and breeding development through pedigree recording, reproductive efficiency, cross-breeding, and genetic modification to produce high-quality buffalo breeding stock. KBuf/TBuf can provide high-quality red meat through improved genetic quality, productivity, and economic management towards modern buffalo breeding development.
Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
Authors: SUSANTO AGUS HERY , Dwiati Murni , Pratiwi Salabila
Published: November 2020

Abstract. Susanto AH, Dwiati M, Pratiwi S. 2020. Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA). Biodiversitas 21: 5164-5169. Ogiera (Eleutheranthera ruderalis) and nodeweed (Synedrella nodiflora) are two different weed species belonging to the family Asteraceae commonly found in tropical regions. At a glance, both species show highly identical morphology, thus leading to difficulty in distinguishing between them. Therefore, molecular data based on particular markers are required. Here, we use an intergenic spacer (IGS) in the cpDNA, i.e., trnT(UGU)-trnL(UAA), as the molecular marker to reveal the difference between the two species. A pair of PCR universal primers, i.e., B48557 as the forward primer and A49291 as the reverse primer, were employed to amplify the marker. Sequence alignment was performed by the use of ClustalW implemented in Bioedit version 7.0.4.1. The results revealed that some differences with respect to both indel and base substitution were observed. Overall, this led to longer IGS trnT(UGU)-trnL(UAA) sequences of E. ruderalis than those of S. nodiflora. Although no direct relationship between the genetic and phenotypic dissimilarities was proven, coincidence seemed likely to exist. This provides molecular evidence that the two phenotypically similar species are genetically different from each other.
Canada and the Free and Open Indo-Pacific: A Strategic Assessment
Authors: Reeves Jeffrey
Published: November 2020

executive summary: This essay assesses the strategic implications for Canada of the “free and open Indo-Pacific” (FOIP) vision and argues that, so long as the concept remains exclusionary, Canada can best achieve its national interests through not adopting this construct. main argumentWith the region’s democratic powers lining up behind the ideal of a free and open Indo-Pacific, Canada faces growing pressure to self-identify as a FOIP proponent state. Yet its rationale for alignment with this vision is not clear. Canada lacks a material presence in the Indian Ocean region necessitating an Indo-Pacific geographic redesign. Further, it stands to gain little through normative alignment with an Indo-Pacific vision predicated on exclusion or containment of China in Asia. Neither does Canada need to align with any FOIP concept to deepen its ties with Australia, India, Japan, or the U.S., all countries with which Canada already has extensive economic, security, and societal ties. Rather, Canada should consolidate and further develop its relations with small and middle powers in the Asia-Pacific. Specifically, its involvement with Latin American states and its participation in institutions like the Comprehensive and Progressive Agreement for Trans-Pacific Partnership present Canada with unrealized opportunity. policy implications• Canada must proactively develop an approach to the Asia-Pacific that allows it to achieve its national interests, including expanded state relations, deepened economic engagement, and increased national prestige. • In rejecting the FOIP constructs, Canada must identify alternative means to engage with Australia, India, Japan, and the U.S. • Canada will retain its ability to engage with China—a key strategic partner—by remaining outside the FOIP construct.
Indonesia’s Indo-Pacific Vision: Staying the Course in a Covid-19 World
Authors: Sambhi Natalie
Published: November 2020

executive summary: This essay examines the evolution of Indonesia’s Indo-Pacific thinking, major developments since the election of President Joko Widodo (Jokowi) in 2014, and the future of the country’s regional leadership amid new 21st-century challenges, particularly Covid-19. main argumentIndonesia’s outlook as a maritime state has matured over the past decade. Under the Jokowi government, important shifts have occurred in the country’s thinking about the Indo-Pacific with implications for maritime policy. In particular, Jokowi has shown a greater focus during his second term on establishing a material legacy—including by upgrading infrastructure, developing human resources, boosting lagging economic growth, and relocating the capital—rather than a visionary one. While Indonesia’s relentless pursuit of multilateralism is necessary to promote regional cooperation and balance strategic rivalry, much of the administration’s attention has been diverted to addressing the economic fallout and public health pressures of the Covid-19 pandemic. Nonetheless, Indonesia’s concerted effort to be an active regional player, given its size and historical nonalignment, remains critical. policy implications• The current Covid-19 crisis and heightened strategic competition in the Indo-Pacific in recent years mean that Indonesia will continue to strenuously pursue multilateralism to balance the influence of both the U.S. and China and to maximize stability and scientific cooperation. • Slowing economic growth and increased strain on the country’s health system due to the pandemic will limit some of Jokowi’s goals for his second term. Yet, given the country’s centrality to the Indo-Pacific construct and the importance of maritime security for the archipelago, the administration cannot afford to divert its focus from the Indo-Pacific. • Indonesia’s failure to develop a coherent response to Indo-Pacific maritime security issues can be traced back to the militarization of its foreign policy. This trend is unlikely to change during the president’s second term, given the prominence of former army generals as advisers in the cabinet.