bluuebunny/crossref_metadata_2025 huggingface dataset:
Giardíase: importância na rotina clínica veterinária
Authors: Ferreira Ana Paula , Destro Flávia Caroline
Published: January 2020

Giardiasis is a routine pathology in the veterinary medical practice, caused by the protozoan Giardia spp, which acts as a source of infection of several species, especially dogs and humans, and is considered a zoonosis of great importance for public health. Young, immunosuppressed dogs attending public places have a higher incidence for the disease. Contamination is due to the ingestion of mature Giardia spp cysts found in the feces of infected animals, water and contaminated food. The need for proper hygiene of the environment, in which dogs reside, in order to avoid contamination with other animals and between humans, is of great importance in the control of giardiasis
Influência da temperatura e umidade sobre o conforto térmico bovino em Barbalha, Ceará
Authors: Lima Mirelle Tainá , FEITOSA JOSÉ VALMIR , OLIVEIRA CARLOS WAGNER , LIMA DA COSTA ANTÔNIO NÉLSON
Published: January 2020

This work aimed to estimate the influence of climatic factors over the cattle milk production in the municipality of Barbalha, ceará, through temperature and relative humidity data to get the temperature and humidity index - hti. Firstly, a thirty-year (1986 to 2016) meteorological data survey was carried out for the municipality of Barbalha-CE, obtained from the automatic meteorological station of the National Institute of Meteorology (INMET), located at the Embrapa Experimental Station in the same city. About the data of dry bulb temperature and relative air humidity three collections were done daily (at 0:00, 12:00 and 18:00 h). The HTI of Barbalha, for dairy cattle, finds in the mostly times a level of HTI that requires attention. Thus, it’s possible to get better the production improving the elements related to Temperature and Humidity. About the studied hours, the 21:00 p.m. represented the best time for thermal comfort, followed by 9:00 a.m. and 3:00 p.m. presented the worst results of thermal comfort. Thus, the producer must pay attention to the favorable time for the cattle management, that will improve the animal welfare and, consequently, milk production.
Histoplasmose nasal em gato: relato de caso
Authors: Rabelo Accioly Maria de Jesus , Ferreira Tiago Cunha , De Morais Glayciane Bezerra , Pinheiro Adriana de Queiroz , Barroso Igor Ciriaco
Published: January 2020

Histoplasmosis is an fungal disease caused by the fungus Histoplasma capsulatum, which is characterized by being dimorphic and saprophyte. This pathology may have acute or chronic clinical character and result in subclinical, pulmonary or disseminated infection. It is characterized by being the second most common mycosis in cats, and in those with low morbidity and high mortality rates. The diagnosis can be obtained by fungal culture and the treatment is mainly based on itraconazole. In order to report a clinical case of feline histoplasmosis, the present study was performed. A four-year-old female cat with no defined breed was treated with a complaint of increased nasal volume. Hematological, biochemical, cytological tests of nasal nodulation, immunochromatographic test for feline viral immunodeficiency and feline viral leukemia were initially requested, and seven days later, fungal culture was requested. Hematological exams showed thrombocytopenia, platelet aggregates, neutrophilia leukocytosis and hypoalbuminemia. Immunochromatographic examination for both retroviruses were negative. On cytopathological examination, yeast structures suggestive of Cryptococcus sp. Were found, but in fungal culture these structures were compatible with Histoplasma capsulatum. Based on the results of the cytological examination, itraconazole 10 mg / kg twice daily for 60 days was prescribed for the patient, which should be started after fungal culture. The animal showed significant improvement after 7 days of drug use, with total nodular regression after 26 days of treatment. After 2 months of treatment, the medication was discontinued for 19 days by decision of the tutor and administration returned for another 30 days in order to prevent the remission of the condition. Thus, it can be concluded that nasal histoplasmosis is a fungal condition present in the clinical routine of felines, thus requiring the veterinarian's attention to symptomatology and complementary diagnostic methods, as well as the correct treatment and prognosis.
SIKAP DAN NIAT BERPENGARUH SECARA LANGSUNG MAUPUN TIDAK LANGSUNG TERHADAP PERILAKU TIDAK MEROKOK REMAJA DI YOGYAKARTA
Authors: Riyadi Sujono , Handayani Sri
Published: January 2020

Positive intentions and attitudes to not smoking are strong predictors of adolescent non-smoking behavior in the future. The purpose of this study was to identify the attitudes and intentions of adolescents to behave non-smoking in Kulon Progo Yogyakarta. Data in the form of questionnaires distributed to 400 adolescents who study in junior high school and senior high school in Kulon Progo Yogyakarta. the data is then analyzed using path analysis using the stata 13 program. The results there is a direct influence on the intention of adolescents not to smoke towards non-smoking behavior of adolescents in the Kulon Progo Regency of Yogyakarta (b = 1.45; 95% CI = 1.45 to 2.49; p <0.001).There is a direct influence on the attitude of adolescents not to smoke towards non-smoking behavior of adolescents in the Kulon Progo Regency of Yogyakarta (b = 1.17; 95% CI = 0.66 to 1.69; p <0.001). the conclusion there is a direct influence of smoking attitudes and intentions on adolescent non-smoking behavior. There is an indirect influence of attitudes through the intention to adolescent non-smoking behavior in Kulon Progo Yogyakarta.
UNIMMA WORK FATIQUE INSTRUMENT (UWFI): SEBUAH INSTRUMENT BARU UNTUK MENGUKUR KELELAHAN KERJA
Authors: Rahayu Heni Setyowati Esti , Rusdjijati Retno , Wijayanti Kartika
Published: January 2020

Work fatigue is a phenomenon that is experienced by many workers that may possibly reduce work productivity among workers. In most cases, this type of fatigue is not just being physically tired, but also it is mentally exhausted. Several instruments which are able to measure this problem has been well developed, however, to the best authors’ knowledge ongoing work fatigue can reduce worker productivity. There are already many instruments to measure fatigue, but each instrument may not be used to measure work fatigue, especially workers in the informal sector, so it is necessary to develop a valid and reliable instrument to measure work fatigue. This research was developed by analyzing every statement in (UWFI). The number of samples was 38 female workers in the informal sector. The statistical test used for validity analysis is product-moment correlation and reliability using single reliability. The results showed that of the 23 items there were 4 items that were invalid but because they were represented by other items, the items were not used, so the UWFI instrument numbered 19 items. While the reliability of the instrument is 0.877, the UWFI instrument is declared valid and reliable.
Detailed description of oviparous females and males of Tubaphis ranunculina (Homoptera: Aphididae) with comments on the species distribution
Authors: Stekolshchikov A.V. , Buga S.V.
Published: January 2020

Detailed descriptions of oviparous females and males of Tubaphis ranunculina (Walker, 1852) (Homoptera: Aphididae) are given. The most complete data on the distribution of this species are overviewed. Acyrthosiphon (Microlophium) ranunculi Mordvilko, 1914 is considered as a new subjective synonym of Acyrthosiphon malvae (Mosley, 1841).
The Foundation for a Smoke-Free World after two years: can it be trusted?
Authors: Etter Jean-Francois
Published: January 2020

BACKGROUND
The Foundation for a Smoke-Free World (FSFW) was launched in 2017 and is funded by Philip Morris International (PMI). We conducted interviews and reviewed documents to evaluate the FSFW and assess whether it can be trusted to produce useful and unbiased research.

ANALYSIS
The FSFW approved grants for USD 156 million since its inception. Several grantees come from low- and middle-income countries, where resources for tobacco control are scarce. The FSFW implements a specific approach that relies on reduced-risk products, agricultural transformation, and transformation of the tobacco industry. Existing tobacco control approaches have had limited impact in some countries, they achieved change too slowly and an acceleration is needed. We shall see whether the Foundation’s original approach can provide this acceleration, but it is too soon the judge its output. The FSFW’s potential is undermined by opposition from the World Health Organization and other tobacco control organizations, and by a difficulty in attracting experienced researchers. It is quite possible that new ideas can come from people outside the field, but inexperienced grantees may have difficulty producing useful work and understanding the importance of independence from the tobacco industry. The decision to obtain funding exclusively from PMI is at the center of the criticism against the Foundation, it undermines trust and creates risks for board members, grantees and staff, some of whom have been harassed by tobacco control people. More transparency on the FSFW’s governance, functioning, grants and grantees is advisable, but obtaining more transparency will require a tougher condemnation of harassment.

CONCLUSIONS
Scrutiny is continuously needed on the Foundation’s independence, governance and output, as for any other scientific or tobacco control effort. The Foundation will ultimately be evaluated on its impact on smokers and poor tobacco farmers and on its ability to support its partners if they are harassed.
Hypoglycemic Effects of Extract Nigella Sativa in Diabetic mice model
Authors: Awwaliya Evi , Salim Hotimah Masdan , Muhammad David Sajid , Romadhani Deny Febriwijaya , Nisaussholihah Nadia
Published: January 2020

Background; Adiponectin is an important adipokine with anti-inflammatory and insulin-sensitizing effects, the circulating levels of which are reduced in individuals with type 2 diabetes and obesity. Indeed, there is evidence that adiponectin released from perivascular adipose tissue (PVAT), that may play a key role in vascular physiology, as bioactive molecules released from it could have direct paracrine effects on the underlying vessel. The present study investigated the effects of extract Nigella sativa (N. sativa) on blood glucose levels of diabetic mice.

Materials; Eight-week-old male musmusculus were treated with alloxan (150 mg/ kg) by a single intraperitoneal injection to induce diabetes mellitus. At 3 days of injection, N. sativa extract (150 and 300 mg/kg) was administered via gavage for 2 weeks. 

Results: The extract of N.Sativa administration significantly reduced blood glucose level (P < 0.05). However, N.Sativa did not alter a body weight

Conclusion:  Extract of Nigella Sativa improved blood glucose level in diabetic mice model.
A Promising Effect of Pravastatin For Reducing Preeclamsia Incidence In High Risk Pregnant Women
Authors: Zata Fariska , Nasir Mohammad
Published: January 2020

Preeclamsia is still a threat in obstetrics because it is the leading cause of maternal death (15-20% in developing countries). Globally, preeclampsia causes 70,000-80,000 pregnant women to die and 500,000 babies die annually1, with increased morbidity such as prematurity and fetal growth disturbance2. The exact cause of preeclampsia is still not clearly known (also called "The disease of theory"), but recent studies shows that the imbalance of pro-angiogenic (VEGF, PlGF) and anti-angiogenic factors (sFlt-1, s-Eng) plays an important role in the pathogenesis preeclampsia. The presence of general maternal endothelial dysfunction induced by an imbalance of these factors is a major phenomenon in preeclampsia, which results in placental hypoxia / ischemia, resulting in vasoconstriction resulting in hypertension1. Termination of pregnancy is still as a definitive therapy for preeclamsia. Therefore, early prevention is necessary in the management of preeclampsia. In 2013, ACOG recommended the administration of low-dose aspirin and calcium 1 gram / day to patients in pregnant women with high risk of preeclamsia3. However, low-dose aspirin is less useful in preventing preeclampsia in patients with a history of previous chronic hypertension4 and not reduce the incidence of term preeclampsia (the incidence of preeclampsia at gestational age above 37 weeks)5,6. This weakness of low-dose aspirin has led to recent research focusing on the prevention of preeclampsia. The similarity between the pathogenesis mechanism of preeclampsia and cardiovascular disease makes pravastatin (a protective therapy in cardiovascular disease before) as a potential agent for preventing preeclampsia7. Therefore, the role of pravastatin for reducing preeclampsia incidence in high risk pregnant women will be discussed in this article.
O niektórych problemach gospodarki niskoemisyjnej
Authors: Jeżowski Piotr
Published: January 2020

Opracowanie wyjaśnia istotę trwałości klimatycznej i równoległego rozumienia kategorii rozwoju zrównoważonego jako koncepcji totalnej dekarbonizacji gospodarki. Promocja rozwoju zrównoważonego opartego na trwałości klimatycznej, ujmowanego w StrategiiEuropa 2020 i w pakiecie 2.0 obejmującym perspektywę 2030 r., stanowi wielkie wyzwanie dla Polski zarówno ze względu na wyjściową strukturę bilansu energetycznego i charakterystykę systemów energetycznych, jak i na wysokie koszty dla gospodarki oraz społeczeństwa. Na tym tle zostały pokazane rozwiązania, głównie o charakterze technicznym, sprzyjające wdrażaniu gospodarki niskoemisyjnej. Poza OZE, poprawąefektywności energetycznej i substytucją węgla z gazem oraz gospodarką skojarzoną nie ma wielu wydajnych rozwiązań technicznych, które z jednej strony „ratują” klimat i wiodą do gospodarki niskoemisyjnej, z drugiej zaś zapewniają minimalne perspektywy dla węgla. W szczególności możliwości redukcji CO2 w procesach spalania węgla są niewielkie, natomiast umiarkowane szanse można wiązać z procesami jego konwersji.
THE EUROPEAN UNION STRATEGY FOR THE BALTIC SEA REGION - AN EXAMPLE OF BETTER AND MORE EFFECTIVE REGIONAL GOVERNANCE
Authors: Śmigerska-Belczak Iwona
Published: January 2020

The international cooperation in the Baltic Sea region, especially between Scandinaviancountries, has a very long tradition that has impacted signicantly on institutionalisationof regional international relations. The current European Union Strategy for the Baltic SeaRegion is the rst macro-regional strategy within the EU, based on the earlier regionalexperience. Its main goal is strengthening cooperation in this region and promotingsustainable development in order to meet common challenges. The Strategy has broughtbetter coordination and effectiveness of the existing cooperation and turned out to bea very efficient and successful tool. Until spring 2014 the Baltic Sea Region was perceivedas a model region for successful transformation, close regional cooperation, trade andinclusive security. However, it is now facing new challenges connected with changingpolitical situation (EU-Russia relations, Brexit, migrations, etc.). The rules and formsof the governance in the Baltic Sea Region have appeared to be eective and sucientin successfully addressing economic, social and political challenges. This is the answerto the question if there is any evidence to acknowledge the rules, forms and ways ofcooperation as a model which possibly could be followed elsewhere. As a result, the EUhas introduced three more macro-regional strategies.
Aktualność koncepcji współporządków Wilhelma Röpkego dla teorii polityki publicznej
Authors: Szulczewski Grzegorz
Published: January 2020

W artykule podjęto próbę ukazania, na ile koncepcja współporządków Wilhelma Röpkego może posłużyć do budowy współczesnej teorii polityki publicznej. Założenie o wzajemnym oddziaływaniu na siebie porządków: prawnego, kulturowego i ekonomicznego stawia przed polityką publiczną określone zadania szerszego przemyślenia konsekwencji działań w sferze publicznej.Z drugiej strony normatywna koncepcja ładu społeczno-gospodarczego zaproponowana przez Wilhelma Röpkego pozwala na ocenę również współczesnej postaci kapitalizmu. W artykule podjęto taką próbę. W ten sposób zostały ukazane problemy, przed jakimi staje obecnie polityka publiczna: przywrócenia porządku wolnościowego w dobie oligopolistyczno-korporacyjno-finansowego kapitalizmu, zwalczania asymetrii w sferzeposiadania własności oraz prowadzenia zrównoważonej polityki imigracyjnej.Normatywna koncepcja ładu społeczno-gospodarczego wyznacza też liczne zadania, jakie powinna realizować polityka publiczna. Sprowadzają się one do spełniania postulatów aksjologicznych, a mianowicie działania zgodnie z wartościami: efektywności, konkurencyjności i wolności gospodarczej, a w sferze społecznej realizacji wartości personalistycznych (godności, wolności osobistej, odpowiedzialności, sprawiedliwości,równości i solidarności). Sama polityka publiczna ma być kształtowana również według zasady subsydiarności i realizować ideę bezpieczeństwa socjalnego.
Polityka państwa w realiach kultury bezrobotnych jako subkultury ubóstwa. Na bazie koncepcji teorii pól Pierre’a Bourdieu
Authors: Konopka Dorota
Published: January 2020

W artykule powiązano perspektywę teoretyczną badań nad kulturą bezrobotnych z dorobkiem francuskiego socjologa Pierre’a Bourdieu, w tym z teorią pól. Pole (le champ) jest jedną z kluczowych kategorii jego teorii. Refleksyjna analiza pojęć teoretycznych P. Bourdieu jest pomocna, aby wyjaśnić przyczyny marginalizacji i wykluczenia na przykładzie długoterminowego bezrobocia. Analiza teoretyczna ukazała, że przyczyny marginalizacji tkwią nie tylko w samych jednostkach jej ulegających, ale także uwarunkowane są przemianami ogólnospołecznymi, strukturą społeczną danego państwa. Analizy Bourdieu są dobrą podstawą do programowania polityki publicznej, której celem jest ograniczanie społecznych i ekonomicznych skutków trwałego bezrobocia.
Krótkookresowe skutki programu Rodzina 500+
Authors: Ruzik-Sierdzińska Anna
Published: January 2020

Głównym celem artykułu jest omówienie dotychczasowego wpływu programu Rodzina 500+ na trzy obszary: liczbę urodzeń, ubóstwo w rodzinach z dziećmi i aktywność zawodową rodziców (zwłaszcza matek) małych dzieci. Wykorzystano dostępne dane statystyczne oraz wyniki kilku badań na ten temat. Wyniki analiz pokazały, że nowe świadczenie obniżyło poziom ubóstwa wśród rodzin z dziećmi, zwiększyło liczbę urodzeń – choć może to być efekt przejściowy i związany także z innych zmianami wprowadzanymi w latach 2013–2016 – a także zmniejszyło aktywność zawodową, głównie kobiet.
Nic o nas bez nas? Zaangażowanie ruchu osób z niepełnosprawnościami w monitoring wdrażania Konwencji ONZ o prawach osób niepełnosprawnych w Polsce. Studium przypadku
Authors: Kocejko Magdalena
Published: January 2020

Artykuł 33 ratyfikowanej w 2012 r. przez Polskę Konwencji ONZ o prawach osób niepełnosprawnych (KPON) wskazuje konieczność prowadzenia działań monitorujących jej wdrażanie przez społeczeństwo obywatelskie, w tym w szczególności organizacje zrzeszające osoby z niepełnosprawnościami. Faktyczna reprezentacja i wpływ środowiska osób z niepełnosprawnościami w myśl zasady „nic o nas bez nas” jest przedmiotem szczególnego zainteresowania Komitetu ONZ o prawach osób niepełnosprawnych, który przygotował komentarz generalny do art. 33 poświęcony tej sprawie. W artykule przeanalizowany został sposób prowadzenia monitoringu KPON w Polsce i włączenie w ten proces osób z różnymi niepełnosprawnościami w kontekście wymagań określonych przez Komitet ONZ. W Polsce monitoring KPON prowadzony jest w dużej mierze przez duże organizacje pozarządowe i grono ekspertów, co ma swoje konsekwencje. Z działań monitoringowych i rzeczniczych praktycznie wykluczone są grupy osób z niepełnosprawnościami narażonych na wielokrotne wykluczenie i dyskryminację, a więc w szczególnie trudnej sytuacji.
Trenerzy wewnętrzni w służbie cywilnej.Warunki skuteczności w świetle modelu AMO zdolności, motywacji, możliwości działania)
Authors: Świetlikowski Łukasz
Published: January 2020

Przedmiotem analizy jest koncepcja trenerów wewnętrznych w służbie cywilnej (urzędach administracji rządowej). Za trenera wewnętrznego uznaje się pracownika służby cywilnej, który poza swoimi podstawowymi obowiązkami uczy innych pracowników z zakresu, w którym się specjalizuje. W badaniu wykorzystano perspektywę zarządzania wiedzą i zarządzania zasobami ludzkimi. Głównym punktem odniesienia jest model AMO, który określa warunki skuteczności zarządzania ludźmi. Zgodnie z nim, skuteczni pracownicy (w tym przypadku trenerzy wewnętrzni) są zdolni (tj. przygotowani do swojej roli), zmotywowani (wewnętrznie lub zewnętrznie) oraz wspierani przez organizację. W artykule przedstawiono działania, jakie może podjąć administracja w każdym z tych zakresów. Zanalizowano również wady i zalety idei trenerów wewnętrznych, a także odniesiono się do kryteriów ich sukcesu. Na tym tle przedstawione zostało studium przypadku – działanie grupy trenerów wewnętrznych w Kancelarii Prezesa Rady Ministrów.
Peruwiański eksperyment. Pomoc rozwojowa poprzez współpracę wielu podmiotów z organizacją pozarządową jako liderem
Authors: Gozdowski Piotr
Published: January 2020

Artykuł ten jest studium przypadku roli, jaką odgrywa organizacja pozarządowa w polityce rozwojowej i pomocy rozwojowej na przykładzie działalności Fundacji Coprodeli w Peru. W rozwiniętych państwach demokratycznych organy władzy państwowej realizację części swoich zadań przekazują organizacjom społeczeństwa obywatelskiego. Jednak organizacje te mogą także stać się liderem polityki rozwoju, zastępując lub wspierając państwo. Ustalenie to jest szczególnie istotne w perspektywie krajów rozwijających się. Jednak model działalności tych organizacji musi być włączający, a nie zastępujący. Wypracowany przez Coprodeli model angażuje zarówno beneficjentów, lokalne i państwowe struktury władzy i biznesu, jak i inne organizacje pomocowe. Współuczestnictwo wielu podmiotów i specyfika pracy fundacji, która stawia całe osiedla w miejscach zaniedbanych i dotkniętych klęskami żywiołowymi, sprawiają, że model wypracowany przez Coprodeli jest ważny dla rozwoju teorii polityki pomocy rozwojowej.
Targeting of plasminogen activator inhibitor-1 activity promotes elimination of chronic myeloid leukemia stem cells
Authors: Takashi Yahata  , Abd Aziz Ibrahim  , Ken-ichi Hirano  , Yukari Muguruma  , Kazuhito Naka  , Katsuto Hozumi  , Douglas E. Vaughan  , Toshio Miyata  , Kiyoshi Ando
Published: January 2020

Therapeutic strategies that target leukemic stem cells (LSCs) provide potential advantages in the treatment of chronic myeloid leukemia (CML). Here, we show that selective blockade of plasminogen activator inhibitor-1 (PAI-1) enhances the susceptibility of CML-LSCs to tyrosine kinase inhibitor (TKI), which facilitates the eradication of CML-LSCs and leads to sustained remission of the disease. We demonstrated for the first time that TGF-β-PAI-1 axis was selectively augmented in CML-LSCs in the bone marrow (BM), whereby protecting CML-LSCs from TKI treatment. Furthermore, the combined administration of TKI plus a PAI-1 inhibitor, in a mouse model of CML, significantly enhanced the eradication of CML cells in the BM and prolonged the survival of CML mice. The combined therapy of imatinib and a PAI-1 inhibitor prevented the recurrence of CML-like disease in serially transplanted recipients, indicating the elimination of CML-LSCs. Interestingly, PAI-1 inhibitor treatment augmented membrane-type matrix metalloprotease-1 (MT1-MMP)-dependent motility of CML-LSCs, and the anti-CML effect of PAI-1 inhibitor was extinguished by the neutralizing antibody for MT1-MMP, underlining the mechanistic importance of MT1-MMP. Our findings provide evidence of, and a rationale for, a novel therapeutic tactic, based on the blockade of PAI-1 activity, for CML patients.
The quiescent fraction of chronic myeloid leukemic stem cells depends on BMPR1B, Stat3 and BMP4-niche signals to persist in patients in remission
Authors: Jeanpierre Sandrine , Arizkane Kawtar , Thongjuea Supat , Grockowiak Elodie , Geistlich Kevin , Barral Lea , Voeltzel Thibault , Guillemin Anissa , Gonin-Giraud Sandrine , Gandrillon Olivier , Nicolini Franck-Emmanuel , Mead Adam J. , Maguer-Satta Véronique , Lefort Sylvain
Published: January 2020

Chronic myelogenous leukemia arises from the transformation of hematopoietic stem cells by the BCR-ABL oncogene. Though transformed cells are predominantly BCR-ABL-dependent and sensitive to tyrosine kinase inhibitor treatment, some BMPR1B+ leukemic stem cells are treatment-insensitive and rely, among others, on the bone morphogenetic protein (BMP) pathway for their survival via a BMP4 autocrine loop. Here, we further studied the involvement of BMP signaling in favoring residual leukemic stem cell persistence in the bone marrow of patients having achieved remission under treatment. We demonstrate by single-cell RNA-Seq analysis that a sub-fraction of surviving BMPR1B+ leukemic stem cells are co-enriched in BMP signaling, quiescence and stem cell signatures, without modulation of the canonical BMP target genes, but enrichment in actors of the Jak2/Stat3 signaling pathway. Indeed, based on a new model of persisting CD34+CD38- leukemic stem cells, we show that BMPR1B+ cells display co-activated Smad1/5/8 and Stat3 pathways. Interestingly, we reveal that only the BMPR1B+ cells adhering to stromal cells display a quiescent status. Surprisingly, this quiescence is induced by treatment, while non-adherent BMPR1B+ cells treated with tyrosine kinase inhibitors continued to proliferate. The subsequent targeting of BMPR1B and Jak2 pathways decreased quiescent leukemic stem cells by promoting their cell cycle re-entry and differentiation. Moreover, while Jak2-inhibitors alone increased BMP4 production by mesenchymal cells, the addition of the newly described BMPR1B inhibitor (E6201) impaired BMP4-mediated production by stromal cells. Altogether, our data demonstrate that targeting both BMPR1B and Jak2/Stat3 efficiently impacts persisting and dormant leukemic stem cells hidden in their bone marrow microenvironment.
Accuracy of chitotriosidase activity and CCL18 concentration in assessing type I Gaucher disease severity. A systematic review with meta-analysis of individual participant data
Authors: Tatiana Raskovalova  , Patrick B. Deegan  , Pramod K. Mistry  , Elena Pavlova  , Ruby Yang  , Ari Zimran  , Juliette Berger  , Céline Bourgne  , Bruno Pereira  , José Labarère  , Marc G. Berger
Published: January 2020

Chitotriosidase activity and CCL18 concentration are interchangeably used for monitoring Gaucher disease (GD) activity, together with clinical assessment. However, comparative studies of these two biomarkers are scarce and of limited sample size. The aim of this systematic review with meta-analysis of individual participant data (IPD) was to compare the accuracy of chitotriosidase activity and CCL18 concentration for assessing type I GD severity. We identified cross-sectional and prospective cohort studies by searching Medline, EMBASE, and CENTRAL from 1995 to June 2017, and by contacting research groups. The primary outcome was a composite of liver volume >1.25 multiple of normal (MN), spleen volume >5 MN, hemoglobin concentration <11 g/dL, and platelet count <100x109/L. Overall, IPD included 1109 observations from 334 patients enrolled in nine primary studies, after excluding 111 patients with undocumented values and 18 patients with deficient chitotriosidase activity. IPD were unavailable for 14 eligible primary studies. The primary outcome was associated with a 5.3-fold (95% confidence interval [CI], 4.2 to 6.6) and 3.0-fold (95% CI, 2.6 to 3.6) increase of the geometric mean for chitotriosidase activity and CCL18 concentration, respectively. The corresponding areas under the receiver operating characteristics curves were 0.82 and 0.84 (summary difference, 0.02, 95% CI, -0.02 to 0.05). The addition of chitotriosidase activity did not improve the accuracy of CCL18 concentration. Estimates remained robust in the sensitivity analysis and consistent across subgroups. Neither chitotriosidase activity nor CCL18 concentration varied significantly according to a recent history of bone events among 97 patients. In conclusion, CCL18 concentration is as accurate as chitotriosidase activity in assessing hematological and visceral parameters of GD severity and can be measured in all GD patients. This meta-analysis supports the use of CCL18 rather than chitotriosidase activity for monitoring GD activity in routine practice.
The Development Impact of PT. Medco E & P Malaka on Economic Aspects in East Aceh Regency
Authors: Shah Mirza Mardhan , Sirojuzilam Sirojuzilam , T Maas Linda
Published: January 2020

Development basically aims to realize prosperity for the community. Development can be done by implementing in various forms of programs. Development that uses human resources and high technology to process natural resources that can be utilized for human life, one of which is the development of oil and gas exploration block A area of PT. Medco E & P Malaka in Indra Makmur Sub-district. This activity is expected to provide positive benefits for the welfare of the people around the exploration activities. The type of research is associative research with a quantitative approach. This research was conducted in the oil and gas exploration area of Block A Area PT. Medco E & P Malaka in Alue Ie Itam and Blang Nisam Villages in Indra Makmur Sub-district, East Aceh Regency. Respondents in the study consisted of 50 respondents. The result of this study is the economic impact of oil and gas exploration development in Block A area of PT. Medco E & P Malaka has a very positive impact on income, home ownership, home conditions, and additional income of the community in Alue Ie Itam Village and Blang Nisam Village. This can be seen from tcount (6.852) which is greater than ttable (1.67591). Significant differences are also indicated by the significance score (0.00 <0.05).
New materials of Early Iron Age from river Luha valley
Authors: Ilchyshyn Zoya , Silayev Oleksandr
Published: January 2020

The article submits the results of protective archaeological studies conducted in 2016–2018 by scientific research center “Rescue Archaeological Service” (National Academy of Sciences of Ukraine, Institite of archaeology) at the Lokachi gas production facility. Excavations have been made on the settlements Markovychi 1, Markovychi 2, Mizhgirya 1, Mizhgirya 3, Rohovychi 1 and Rohovychi 2 which contained materials of Early Iron Age. Until recently only three sites of that period were discovered in Lokachi district, undoubtedly a minor achievement especially by comparison with fruitful studies made by L. Krushelnytska, G. Okhrimenko, I. Mykhalchyshyn, V. Konoplia and S. Terskyi across the neighboring Volodymyr-Volynski, Ivanychi and Lutsk districts of Volyn region. Therefore, any new excavation results would make an essential influx of information on Early Iron Age settlement structure in the valley of river Luga.
The article analyses items, mostly handmade pottery from cultural layers and archaeological objects, often found alongside with another materials of different cultural and chronological origins. The largest item collections have been discovered on the settlements Rohovychi 1 and Mizhgirya 3 which included household structures full with pottery of distinctive forms and décor elements that is of so called Lesznytsia type of Early Scythian time culture. Shapes and technological characteristics of local pottery resemble peculiar features of same item category on the Early Iron Age archaeological sites from neighboring Rivne and Lviv regions. On contrary they miss the same features on the synchronous sites in the southern parts of Volyn region. This certainly provokes issues regarding the inner transfers and cultural conversation among the populaces of Lezhnytsia type group.
Seemingly small-scale survey studies and excavations have produces substantial results but additionally they announced great prospects of further regional studies important for evaluation of migratory movements and settlement evolution in the area on the ridge crossing between the basins of Western Bug and Pryp’iat revers.
Key words: Early Iron Age, Lezhnytsia type group, Lokachi gas production facility, archaeological surveys, settlement, pottery.
University archaeology of interwar Lviv through the prism of site protection legislation in 1928
Authors: Bulyk Natalia
Published: January 2020

Field archeological activity of Lviv University during the interwar period is considered through the site protection legislation of the Second Commonwealth. There were two archaeological chairs at the university during the period under study – one of prehistory and one of classical archaeology. In fact, the Chair of the prehistory served as site protection institution. All reports of archaeological finds were sent at the same time to the conservator Zbigniew Hornung and to the heads of the chair. So, archaeologists of the University, depending on their scientific interests, visited these sites. The most active field research was conducted by T. Sulimirski and M. Smishko, and some field activity was also carried out by K. Żurowski, H. Cehak and others. During the period of 1928–1939 a huge number of sites were discovered and explored in the western part of Ukraine. Among them Mali Gribovychi and Kulchytsi in Lviv region, Stopchativ, Trach, Komariv and Bukivna in Stanislaviv region are worth to mention. Most of them are burial sites. First of all, they were reflected in addition to publications in the documents of “Office of Conservation of Lviv Voivodeship”. These are permissions, brief information reports and short notes about finds from different locations.
At the same time, documents which confirmed conducting of field studies by the Chair of the Classical Archeology were preserved. Taking into account that the main area of interest of the Chair was ancient civilizations, its employees carried out much less archaeological studies on our territory. However, almost every year E. Bulanda, K. Majewski and I. Startchuk obtained permission for excavation or surveys. The joint work of both Chairs deserves special attention. For example, in summer of 1937, surveys were carried out on the territory of Borshchiv powiat (district) with the participation of young archaeologists M. Smishko, I. Starchuk and other employees of the Chair. The expedition was quite successful and its results have been published in Lviv journals, in particular in “Eos”.
Key words: reform of 1928, site protection legislation, Chair of the Prehistory, Chair of the Classical Archeology, permission on field researches, burial sites.
Research on Vedmezhe settlement in Romny region: Mykola Makarenko's excavations
Authors: Nimenko Nataliia
Published: January 2020

The article is devoted to the study of the little-known biography pages of the famous archaeologist, art critic, organizer of the museum activity M. Makarenko (1877–1938). Shot as a public enemy for his steady position on the protection of historical and cultural monuments, the scientist had left a number of publications covering various areas of his activity, as well as dedicated to specific architectural and archaeological sites.
However, not all the ideas of the scientist have been implemented. Archival documents kept at central and regional institutions, museum funds include materials that reveal plans to continue exploring particular archaeological sites and writing certain publications.
For several decades, M. Makarenko studied archaeological sites in Romny region (territory of modern Sumy region), which gave grounds to speak about opening of a new archaeological culture, now known as Romny culture. Four settlements were the most interesting from a scientific point of view: Monastyrysche, Vedmezhy, on the khutirs of Vashkevych and Hlynske. Fundamental work of the scientist, devoted to Romny archaeological culture, is “Monastyrysche” settlement. However, according to the documents, the scientist also planned a separate publication of the materials from the excavations of the site Vedmezhe. The plans were not implemented. Drafts were also not found.
In the article on the basis of archival documents, M. Makarenko’s publications and drawings, the attempt to reproduce the chronology of the study of Vedmezhe settlement from 1906 to the end of the 1920s is made; the main results of the excavations are highlighted. The main factors that influenced the effectiveness of scientific research in the 1920s are shown.
During excavations of Vedmezhe settlement, M. Makarenko for the first time applied a new technique – burial scraper, which the scientist later used when studying archaeological sites near Sumy and Mariupol.
Key words: research, archaeological site, settlement, excavations, plan, drawing, pottery.
Polygenic Hypercholesterolemia: Examples of GWAS Results and Their Replication in the Czech-Slavonic Population
Authors: HUBACEK J. A. , ADAMKOVA V. , LANSKA V. , DLOUHA D.
Published: January 2020

Since 2007, the year of their first widespread use, genome-wide association studies (GWAS) have become the “gold standard” for the detection of causal genes and polymorphisms in all fields of human medicine. Cardiovascular disease (CVD), one of the major causes of morbidity and mortality, is no exception. The first GWAS focused on hypercholesterolemia and dyslipidemia as the major CVD determinants. GWAS confirm the importance of most of the previously identified genes (e.g. APOE, APOB, LDL-R) and recognize the importance of new genetic determinants (e.g. within the CILP2 or SORT1 gene clusters). Nevertheless, the results of GWAS still require confirmation by independent studies, as interethnic and interpopulation variability of SNP effects have been reported. We analyzed an association between eight variants within seven through GWAs detected loci and plasma lipid values in the Czech post-MONICA population sample (N=2,559). We confirmed an association (all P<0.01) between plasma LDL-cholesterol values and variants within the CILP2 (rs16996148), SORT1 (rs646776), APOB (rs693), APOE (rs4420638) and LDL-R (rs6511720) genes in both males (N=1,194) and females (N=1,368). In contrast, variants within the APOB (rs515135), PCSK9 (rs11206510) and HMGCoAR (rs12654264) genes did not significantly affect plasma lipid values in Czech males or females. Unweighted gene score values were linearly associated with LDL-cholesterol values both in males (P<0.0005) and females (P<0.00005). We confirmed the effects of some, but not all analyzed SNPs on LDL-cholesterol levels, reinforcing the necessity for replication studies of GWA-detected gene variants.
Molecular Genetic Background of an Autosomal Dominant Hypercholesterolemia in the Czech Republic
Authors: TICHÝ L. , FAJKUSOVÁ L. , ZAPLETALOVÁ P. , SCHWARZOVÁ L. , VRABLÍK M. , FREIBERGER T.
Published: January 2020

Autosomal dominant hypercholesterolemia (ADH), more known as familial hypercholesterolemia (FH), is a lipid metabolism disorder characterized by an elevation in low-density lipoprotein cholesterol (LDL-C) and increased risk for cardiovascular disease. In this study, we assessed a spectrum of mutations causing ADH in 3914 unrelated Czech patients with clinical diagnosis of hypercholesterolemia. Samples have been collected within the framework of the MedPed project running in the Czech Republic since 1998. So far we have found 432 patients (11.0 %) with the APOB gene mutation p.(Arg3527Gln) and 864 patients (22.1 %) with the LDLR gene mutation. In 864 probands carrying the LDLR gene mutation, 182 unique allelic variants were detected. We have identified 14 patients homozygous for mutations in the LDLR or APOB genes. We performed function analyses of p.(Leu15Pro) and p.(Gly20Arg) sequence variations.
La gestión pedagógica en la mejora del desempeño docente
Authors: Quispe-Pareja Marleni
Published: January 2020

El presente trabajo de investigación buscó demostrar cómo la gestión pedagógica del directivo se relaciona con el desempeño docente en el nivel secundario de la Institución Educativa Privada “Bertolt Brecht” del distrito Cercado de Lima, 2017. Es una investigación cuantitativa, con diseño correlacional de corte transversal. La muestra fue de 234 estudiantes, 49 docentes y 9 directivos a quienes se les aplicó una encuesta. La validación se realizó a partir del juicio de expertos con un resultado de 92%; además se empleó la prueba el Alfa de Cronbach dando como resultado 0,819, observándose que luego de su aplicación, los resultados tienen una excelente validez. La hipótesis alterna sostiene que existe una relación significativa entre la gestión pedagógica de directivo y el desempeño docente en el nivel secundario de la institución en estudio. Para la contrastación de hipótesis se utilizó la correlación de Pearson entre las variables: gestión pedagógica y desempeño del docente. Como el valor p = 0.000 < 0.05, se rechaza la hipótesis nula, y se acepta la hipótesis alterna. Por lo tanto ambas variables tienen una correlación directa y alcanza un nivel de 0.576 que corresponde a un nivel correlación positiva. Se concluye que a una óptima gestión pedagógica del directivo, mayores serán los niveles de desempeño de los docentes. De manera contraria, si la gestión pedagógica del directivo no es priorizada, el desempeño de los docentes será limitado y con deficiencias.
Role of Agriculture in ensuring Food Security
Authors: Kaini Malati
Published: January 2020

The 2007-2008 food crises and the renewed surge in food prices in 2010-2011 have exposed deep structural problems in the global food system and the need to increase food production. The World Food Summit of 1996 defined food security as existing "when all people at all times have access to sufficient, safe, nutritious food to maintain a healthy and active life". Despite Nepal has no worse food and nutrition security situation among SAARC countries, there are some districts where food is always in shortage. The explicit constitutional recognition of the right to food and other related provisions in articles 36 and 42 of the new constitution will be instrumental for implementing food security programs in the country. Food security for poor people is based on sustainable food production. Many proven technologies and improved practices have the promise to boost agricultural production and ensure food security in developing countries like Nepal. Food and Nutrition security program (FANUSEP) is one of the flagship programs of Agriculture Development Strategy 2015-2035 (ADS), which is in the first phase of its implementation. It aims at improving the food and nutrition security of the most disadvantaged groups of the population.
Political Science Internships: A Path to "Workforce Ready" without Selling Out
Authors: Foster Shoaf Nicole
Published: January 2020

This paper examines approaches to launching or retooling internship programs for undergraduate political science programs, and how to structure, leverage, and assess internship programs for maximum impact. I review the existing literature on internships in political science and offer insight from the overhauling of an internship at a regional state university.
Towards a Multi-Temporal Deep Learning Approach for Mapping Urban Fabric Using Sentinel 2 Images
Authors: El Mendili Lamiae , Puissant Anne , Chougrad Mehdi , Sebari Imane
Published: January 2020

The major part of the population lives in urban areas, and this is expected to increase in the future. The main challenges faced by cities currently and towards the future are the rapid urbanization, the increase in urban temperature and the urban heat island. Mapping and monitoring urban fabric (UF) to analyze the environmental impact of these phenomena is more necessary than ever. This coupled with the increased availability of Earth observation data and their growing temporal capabilities leads us to consider using temporal features for improving land use classification, especially in urban environments where the spectral overlap between classes makes it challenging. Urban land use classification thus remains a central question in remote sensing. Although some research studies have successfully used multi-temporal images such as Landsat-8 or Sentinel-2 to improve land cover classification, urban land use mapping is rarely carried using the temporal dimension. This paper explores the use of Sentinel-2 data in a deep learning framework, by firstly assessing the temporal robustness of four popular fully convolutional neural networks (FCNs) trained over single-date images for the classification of the urban footprint, and secondly, by proposing a multi-temporal FCN. A performance comparison between the proposed framework and a regular FCN is also conducted. In this study, we consider four UF classes typical of many European Western cities. Results show that training the proposed multi-date model on Sentinel 2 multi-temporal data achieved the best results with a Kappa coefficient increase of 2.72% and 6.40%, respectively for continuous UF and industrial facilities. Although a more definitive conclusion requires further testing, first results are promising because they confirm that integrating the temporal dimension with a high spatial resolution into urban land use classification may be a valuable strategy to discriminate among several urban categories.
Identification of Echinacea Purpurea (L.) Moench Root LysM Lectin with Nephrotoxic Properties
Authors: Balciunaite Gabriele , Haimi Perttu-Juhani , Mikniene Zoja , Savickas Girius , Ragazinskiene Ona , Juodziukyniene Nomeda , Baniulis Danas , Pangonyte Dalia
Published: January 2020

Echinacea purpurea (L.) Moench (EP) is a well-studied plant used for health benefits. Even though there are a lot of data on EP secondary metabolites, its active proteins are not studied well enough. The aim of our experiment was to purify lectin fraction from EP roots and evaluate its biological activity in vitro as well as its effect on kidney morphology in vivo. An EP root glycoprotein fraction was purified by affinity chromatography, identified by LC-MS/MS, and used for biological activity tests in vitro and in vivo. Identified glycoproteins were homologous with the LysM domain containing lectins from the Asteraceae plants Helianthus annuus L., Lactuca sativa L., Cynara cardunculus L. A purified fraction was tested by hemagglutination and hemagglutination inhibition (by carbohydrate reactions) in vitro. We purified the hemagglutinating active ~40 kDa size lactose, D-mannose, and D-galactose specific glycoproteins with two peptidoglycan binding LysM (lysine motif) domains. Purified LysM lectin was tested in vivo. Eight-week old Balb/C male mice (n = 15) were treated with 5 μg of the purified lectin. Injections were repeated four times per week. At the fifth experimental week, animals were sedated with carbon dioxide, then euthanized by cervical dislocation and their kidney samples were collected. Morphological changes were evaluated in hematoxylin and eosin stained kidney samples. The purified LysM lectin induced a statistically significant (p < 0.05) kidney glomerular vacuolization and kidney tubular necrosis (p < 0.001).