bluuebunny/crossref_metadata_2025 huggingface dataset:
Sex differences in sucrose-induced locomotor sensitization and cross-sensitization with the D2/D3 agonist quinpirole in rats
Authors: Matic Vangel , Huynh Matthew , Bishnoi Indra R. , Kavaliers Martin , Ossenkopp Klaus-Peter
Published: September 2024

Background: Female-biased sex differences are known to exist in the prevalence of eating-related disorders and the experience of food cravings. Similarly, in rodents, females display a greater preference and motivation for the highly palatable sweet food, sucrose, than do males. Locomotor sensitization, the increase in locomotor activity that occurs with repeated administrations of drugs of abuse, is thought to occur through drug-induced sensitization of the mesolimbic dopamine pathway and reflect enhancements in drug craving. Pre-exposure to sucrose has been shown to enhance locomotor sensitization induced by dopaminergic agonists, however, it is unknown if sex differences exist in this effect.
Methods: Female (n = 16) and male (n = 16) Long-Evans rats received 30 min daily access to sucrose (0.3 M) or water for nine consecutive days followed by daily administration of the D2/D3 agonist, quinpirole (0.5 mg/kg), for nine consecutive days. On the first, fifth, and ninth day of the sucrose and quinpirole phases, rats were placed in automated activity monitors for 30 min after fluid access and drug administration, respectively, to assess locomotor activity. Mixed model analyses of variance were used to evaluate differences in locomotor activity between Female + Sucrose, Female + Water, Male + Sucrose, and Male + Water treated rats.
Results: Female + Sucrose rats showed significantly greater weight-adjusted fluid consumption of sucrose than did Male + Sucrose rats. Female + Sucrose rats exhibited a significant increase in locomotor activity across the sucrose phase and significantly greater locomotor activity relative to other groups. In the quinpirole phase, both groups pre-exposed to sucrose (Female + Sucrose, Male + Sucrose) showed greater activity than their same-sex control pre-exposed to water (Female + Water, Male + Water). Further, both female groups generally displayed greater activity than both male groups in the quinpirole phase.
Conclusions: A female-biased sex difference was identified in the effect of locomotor sensitization induced by sucrose (0.3 M), but not for sucrose pre-exposure on quinpirole sensitization (0.5 mg/kg). These results suggest that differences may exist between sexes in the mesolimbic response to sucrose, perhaps underlying sex differences in food cravings and eating-related disorder prevalence.
Nursing Students' Perception and Associated Factors Towards the Nursing Profession at Selected Health Science Colleges in Addis Ababa, Ethiopia: A Cross-sectional study.
Authors: Hassen Rahmet , Habte Teshome , Dugassa Boka
Published: September 2024

Background: Understanding nursing students' perceptions of their profession is crucial issue for improving educational programs, enhancing support systems, and ultimately preparing competent and compassionate health care professionals.
Objective: This study aimed to assess nursing students' perceptions and the associated factors of the nursing profession at selected health science colleges in Addis Ababa, Ethiopia.
Methods: A cross-sectional study was conducted from Feb 19- Mar 19, 2024, among nursing students at four health science colleges. Data was collected by using structured interview questionnaires after preparation and validated by using expert review from randomly selected undergraduate Nursing students enrolled in the four health science colleges of Addis Ababa. And organized students' socio-demographic characteristics, perceptions towards nursing, and associated factors and presented Descriptive statistics, Bivariate and multiple logistic regression analysis were computed using SPSS version 26.
Results: A total of 267 nursing students participated in this study yielding a 100% response rate. The majority (57.3%) of the students had a positive perception of the nursing profession. Factors significantly associated with positive perception included female gender, higher family income, and having a close family member who is a nurse. Factors associated with negative perception included poor clinical supervision and inadequate resources in clinical settings. From this, 82% (n=219) of the study participants were female nurses. Being female (AOR = 5.77; 95% CI: 2.47 – 13.44), competitive salary like other professions (AOR = 3.48; 95% CI: 1.81 – 6.66), having reasons for enrolling in the nursing profession (AOR= 4.39; 95% CI:2.37– 8.13) were significantly associated with the perception of nurses towards their profession.
Conclusion: Nursing students generally had a positive perception of the nursing profession. However, factors related to the clinical learning environment heavy workload, lack of professional autonomy, and poor remuneration was associated with a negative perception. Addressing these factors through improved clinical placements, and awareness creation towards the nursing profession should be implemented before and after students join the nursing school.
MRI-ASL-based CBF changesduring the interictal period after anti-CGRP therapy in migraine patients
Authors: Shimoda Masami , Hoshikawa Kaori , Oda Shinri , Imai Masaaki , Aoki Rie , Shinohara Chiaki
Published: September 2024

Objective: Migraine and insomnia often accompany migraine are not steady-state conditions because cerebral blood flow (CBF) autoregulation and the glymphatic system are impaired. Anti-endogenous anti-calcitonin gene-related peptide (CGRP) therapy may thus reduce the interictal CBF.
Methods: Interictal magnetic resonance arterial spin labeling (ASL) was performed on 73 migraineurs before and after anti-CGRP therapy, including antibody switching, to investigate changes in CBF and predictors of treatment response.
Results: Multivariate analysis revealed that the significant positive neuroradiological predictors were no migraine with cortical hyperperfusion (CHP) findings despite insomnia and no insomnia with white matter hyperintensities. Patients in this study were divided into four clinical groups according to CBF changes after anti-CGRP therapy: Group A, CHP findings before treatment and decreased CBF after treatment (27 patients); Group B, no CHP before treatment and decreased CBF after treatment (19 patients); Group C, no CHP before treatment and increased CBF after treatment (18 patients); and Group D, no change in CBF before or after treatment. Changes in CBF after therapy were appropriate in Groups A and C; in particular, Group C had the highest percentage of ³ 50% of the responders, reaching 94%. Approximately 90% of patients in Group B showed an inappropriate reduction in CBF below normal after treatment. All but 1 patient in Group D did not respond to treatment.
Conclusion: Interictal CBF measurements by ASL before and after anti-CGRP therapy are clinically useful biomarkers for predicting and evaluating treatment efficacy.
The Anatoly Silantiev Memorial Readings, dedicated to the 150-th anniversary of the birth: Game management in Russia. History and modernity
Authors: Селиховкин А.В. , Масайтис В.В. , Николаева М.А. , Варенцова Е.Ю.
Published: September 2024

Конференция проводится кафедрой защиты леса, древесиноведения и охотоведения при поддержке Санкт-Петербургского государственного лесотехнического университета имени С.М. Кирова.
Algorithms for topical therapy of mycoses at the daily appointment of a dermatologist
Authors: Silina L. V.
Published: September 2024

This article discusses the immediacy of the problem of rising incidence of common mycoses that dermatovenerologists deal with at outpatient visits, the reasons for their occurrence, conversion from one course of the disease to another and the use of etiological effective treatments. The author described the mechanisms of development of different mycoses according to their etiology, comorbid disorders accompanying mycoses and the features of the clinical picture based on etiological factors and sites of infection. The article places special emphasis on the reasons for infection with fungal invasions in humans, conditions and numerous contact routes. Modern features of the course of fungal skin diseases include formation of biofilms in the sites of infection, multicentricity, extension of the pathological process, frequent formation of granulomas, follicles, occurrence of numerous mycoides, as well as the low virulence of dermatophytes. The publication also presents various clinical cases of somatically healthy patients suffering from various mycoses caused by variant factors, which have pathological processes located in different sites. According to the doctor’s prescription, patients used conventional treatment regimens that included external/ topical treatment and general therapy, strictly in accordance with clinical guidelines – the use of highly active broad-spectrum antifungal drugs with fungicidal, fungistatic, antibacterial, anti-inflammatory and antipruritic action. These properties ensure a low probability resistance of microorganisms or relapses during treatment with topical dosage forms of antifungal drugs for cutaneous application. Upon completion of the course of treatment, patients reported a significant improvement in their skin condition resulting in minimization of disease symptoms, disappearance of skin rashes, skin itching, and elimination of exfoliation.
Immunologic markers for vitiligo
Authors: Petunina V. V.
Published: September 2024

Questions of the etiology and pathogenesis of vitiligo remain open to this day. Of the majority of hypotheses for the development of vitiligo, the leading role today belongs to the autoimmune theory. The most relevant for solving issues of both therapy and diagnosis of the disease are studies devoted to the immunological mechanisms accompanying the development of vitiligo. The article describes both immunological markers of autoimmune diseases of satellites and changes in immunity during the disease itself. The question of the activity of the process sometimes causes great difficulties, both for the doctor, and the patient himself sometimes cannot say for sure whether there is a growth of foci, especially with a widespread process. In the article we touched upon the issues of consideration and immunological markers, which, according to pilot studies, can serve as laboratory indicators of activity. The roles of interleukin 17, imbalance between subpopulations of T-helper lymphocytes type 1 (Th 1) or Th 17 and Tregs and Th 2, cytokine imbalance, JAK1 and JAK2 kinases, CXCL 9, CXCL 10, granzyme B are described.
Comparative analysis of Health-Related Quality of Life, anxiety and depression in patients with alopecia areata, atopic dermatitis and with association of these diseases
Authors: Tereshchenko G. P. , Gadzhigoroeva A. G. , Romanova Yu. Yu. , Potekaev N. N.
Published: September 2024

Introduction. Alopecia areata (AA) and atopic dermatitis (AtD) are associated with significant psychosocial burden, emphasizing the importance of quality of life (QoL) assessment due to potential psychological distress and treatment hindrance.

Aim. To conduct a comparative analysis of QoL, anxiety and depression in patients with AA, AtD and their combination, according to diseases severity.

Materials and methods. The study included 91 patients of both sexes (18–52 years old). All patients were divided into 3 groups: group 1 – 25% patients with AA, group 2 – 28% with AtD, group 3 – 47% patients with both diseases; the groups were subdivided by dermatoses severity. The DLQI, Skindex-29 and HADS questionnaires were used. Mann-Whitney U-test was conducted to compare the mean values of quantitative data (М ± n) (p < 0.05).

Results. Group 1 had moderately decreased QoL according to DLQI (6.5 ± 0.67); total Skindex-29 score was 23.55 ± 2.46 (low impact) with the highest value in the emotion domain. In group 2 DLQI score was 12.5 ± 1.51, total Skindex-29 score was

48.41 ± 3.76; in group 3 DLQI was 11.5 ± 0.58, Skindex-29 – 50.46 ± 2.14. These values corresponded to very negative impact on QoL, increasing with diseases severity. There was a reliable difference in DLQI and Skindex-29 values between groups 1 and 2 and groups 1 and 3. Mean HADS scores in all groups were generally comparable and correlated with AtD and AA severity.

Conclusion. Compared to AA, AtD has a greater impact on QoL, involving all areas of social health; AA predominantly affects emotional sphere. The presence of both diseases worsens QoL as well as AtD alone, especially in aspects “emotions” and “functions”.
Netakimab in the treatment of psoriatic erythroderma
Authors: Tarasenko G. N. , Patronov I. V.
Published: September 2024

Psoriasis is considered as system immunoassociated disease of the multifactorial nature with dominating value in development of genetic factors. Suffer псориазом from 2 to 7% of inhabitants of a planet. Psoriasis it is started, when environment factors activate plasmocytoid dentrid cages that leads to development numerous proinflammatory citokines, including the factor некроза to a tumour FNO-α, interferon (IFN) γ, interleukin (IL) 17, IL-22, IL-23 and IL-1β. Many of these citokines stimulate hyperproliferation keratinocyes which supports a chronic inflammation. The heaviest form psoriasis – psoriatic erythroderma, results adverse экзогенных from the factors, irritating treatment. Develops erythroderma at 1.5–3% of patients псориазом which amazes all surface of an integument. All skin cover gets brightly red colour, with a brown shade, is strained. Taking into account recent achievements in understanding of the inflammatory nature psoriatic research efforts have been concentrated to finding-out of a role specific proinflammatory cytokines which participate in pathogenesis diseases, for the purpose of working out of new purposeful methods of treatment. For treatment psoriatic erythroderma in medicine there is a wide spectrum of external and system means. Undoubtedly, psoriatic erythroderma has all indications to appointment of biological therapy, especially when does not give in traditional methods. Authors of article have presented a therapy material psoriatic erythroderma, a domestic preparation of genno-engineering biological therapy of (netakimab). The good clinical effect that opens prospect of application of this preparation is gained. The concrete clinical example of successful treatment is resulted.
New topical combinations in acne therapy
Authors: Katkhanova О. A.
Published: September 2024

Introduction. Therapeutic treatment modalities for acne involve both topical and oral medications. Fixed-dose combinations that affect all components of the pathogenesis of acne vulgaris are given preference among topical medications.

Aim. To evaluate the efficacy and tolerability of Metrogyl® a topical drug, containing adapalene and metronidazole, which is used as topical therapy in patients with mild to moderate acne.

Materials and methods. A prospective, open-label, randomized, single-centre comparative study was conducted in 2022. A total of 60 patients were subjected to the Mir-O-Med clinic-based observation (Krasnodar): 19 men and 41 women aged 18 to 45 years with mild to moderate acne. Group 1 received a fixed-dose combination of adapalene 1.0 mg and metronidazole

10.0 mg (Metrogyl® A); Group 2 – a fixed-dose combination of adapalene 0.1% and clindomycin phosphate 1%; Group 3 – a fixed-dose combination of adapalene 0.1% and benzoyl peroxide 2.5% gel; Group 4 – adapalene 0.1%. Group 5, a separately created group, included patients from Group 1, who continued therapy for another 3 months.

Results. After therapy, 6 (40%) patients from Group 1 archived clinical remission, and 9 (60%) patients found significant improvement. No deterioration in patients’ condition or absence of clinical effects was observed during therapy. The combination of adapalene and metronidazole (Metrogyl® A) to treat mild to moderate acne is comparable in terms of efficacy to the combination of adapalene and benzoyl peroxide, 10% more efficient than topical adapalene therapy and 30% more efficient than topical antibiotics combination.

Conclusion. The obtained results showed high therapeutic efficacy, good tolerability and safety of Metrogyl® A, which allows to use it as monotherapy and as part of combination with other agents in patients with mild to moderate acne.
ÍNDICE DE ANOMALIA DE CHUVA PARA DIFERENTES MESORREGIÕES DO ESTADO DE PERNAMBUCO
Authors: Alves Jandelson de Oliveira , Pereira Poliana de Caldas , De Queiroz Maria Gabriela , Da Silva Thieres George Freire , Ferreira Jadna Mylena da Silva , Araújo Júnior George do Nascimento
Published: September 2024

Uma das formas de estudar a precipitação pluviométrica é através de Índices de Anomalia de Chuva (IAC), que ajudam a monitorar anos de seca e chuva excessiva, permitindo averiguar os impactos que o clima global causa sobre a distribuição pluviométrica do local. Nesse contexto, o presente trabalho objetivou analisar IAC em quatro municípios de diferentes mesorregiões do Estado de Pernambuco, evidenciando os anos de seca e de chuva. Para realização desta pesquisa, foram selecionados os municípios: Barreiros, Caruaru, Floresta e Petrolina, das mesorregiões da Zona da Mata, Agreste, Sertão Pernambucano e Sertão do São Francisco, respectivamente. Os dados de precipitação das séries históricas de cada localidade foram obtidos na Agência Pernambucana de Águas e Clima. Com os dados, foram realizados os cálculos do IAC positivo e negativo, com base na normal climatológica e médias dos 10 anos de máximas e mínimas precipitações e, em sequência, houve a classificação de anos secos e úmidos de acordo com as classes de intensidade do IAC.  Dentre os municípios analisados, Barreiros foi o mais úmido, reflexo da precipitação média anual (2026,79 mm) com o IAC variando entre -5,76 e 6,03. Para Caruaru, ocorreram 33 anos com desvios negativos e 25 com desvios positivos e o IAC variou entre -3,99 e 4,06. Em Floresta, o IAC variou entre -4,82 e 7,69 e ocorreu 1 ano extremamente úmido e 2 extremamente secos. E, em Petrolina, ocorreram 5 anos extremamente úmidos e 1 extremamente seco, sendo que o IAC oscilou entre 5,31 e 7,24. Conclui-se que a utilização do IAC, positivos e negativos, para as cidades analisadas, garante um resultado mais preciso do índice, refletindo em um planejamento agrícola mais elaborado para essas cidades. Sendo que a elevada quantidade de anos que apresentaram IAC negativo, para esses municípios, somada a significativa oscilação entre os anos úmidos e secos das series históricas, caracterizam essas localidades como instáveis para a produção agrícola de sequeiro.
The impact of the phosphorus limitation on denitrification efficiency and specific sludge production in industrial wastewater treatment
Authors: Stanek Radim , Zábranská Jana
Published: September 2024

Heterotrophic denitrification is applied to the treatment of industrial wastewater from nitrated products with high concentrations of residual nitric and sulphuric acids and without organic matter. Wastewater with a concentration of nitrate nitrogen of 100–400 mg/L needs to be neutralized, methanol is used as an organic substrate, and phosphoric acid is used as a nutrient supplement. The pilot model of continuous denitrification was constructed with real wastewater influent. Experiments with different dosages of phosphorus 0–16.8 mg/g P/N-NOx were carried out to determine the effect of phosphorus limitation on the efficiency of denitrification. The minimum phosphorus concentration of 0.24 mg/L, representing a specific dose of P to remove oxidized nitrogen of 1.8 mg/g for sufficient denitrification efficiency was found after a sufficient time of adaptation of 70 days of the denitrification culture to low phosphorus concentrations in the reactor. Long-term successive reduction of phosphorus supply led to an increased proportion of organisms within the denitrifying sludge with lower phosphorus requirements. The results indicated that potential savings could be achieved by reducing phosphoric acid demand although limited phosphorus supply did not reduce specific sludge production.
Features of Gene Regulation in Violation of the Inflammatory
Response of Monocyte-like Cells Bearing Mitochondrial Mutations
Associated with Atherosclerosis
Authors: Orekhov Alexander N. , Nikiforov Nikita G. , Zhuravlev Alexander D. , Verkhova Svetlana S. , Omelchenko Andrey V. , Borodko Daria D. , Sukhorukov Vasily N. , Sinyov Vasily V. , Sobenin Igor A.
Published: September 2024

Aim:

This research aimed to study the features of gene regulation of the inflammatory response in cells carrying mitochondrial mutations associated with atherosclerosis.

Background:

Inflammation plays an important, if not decisive, role in the occurrence of
atherosclerotic lesions and then accompanies it throughout its further development. Thus,
atherogenesis is a chronic inflammatory process. Chronification of inflammation is a consequence of disruption of the normal inflammatory response at the cell level of the vascular
wall.

Objectives:

In this study, we used cytoplasmic hybrids or cybrids carrying atherosclerosis-associated mitochondrial mutations to study gene regulation of inflammatory response.
The main goal of the study was to identify the key genes responsible for the impaired inflammatory response revealed for some cybrids.

Methods:

Inflammatory stimulation of cybrids was induced with bacterial
lipopolysaccharide, and assessed through secretion of pro-inflammatory cytokines CCL2,
IL8, IL6, IL1b. A transcriptome analysis was performed to identify the key genes (master
regulators) in the normal (tolerant) and intolerant response of cybrid cells

Results:

Normal inflammatory response after re-stimulation elicited a much smaller secretion of pro-inflammatory cytokines. In an intolerant response, the level of secretion upon
re-stimulation was the same or even higher than after the first stimulation. Normal and intolerant responses differed significantly both in terms of the number of signaling pathways
involved and qualitatively, since the signaling pathways for normal and intolerant responses are completely different. Master regulators controlling normal and intolerant inflammatory response were identified. For a normal response to the first inflammatory stimulation, no
common master up-regulators and 3 master down-regulators were identified. The reverse situation was observed with the intolerant inflammatory response: 6 master up-regulators,
and no master down regulators were identified. After the second inflammatory stimulation,
no master regulator common to all studied cytokines was found. Thus, key genes involved
in the development of intolerant inflammatory response have been identified. In addition,
other key genes were identified that were initially associated with an intolerant inflammatory response and thus determine disorders of the inflammatory reaction leading to chronification of inflammation.

Conclusion:

We identified disturbances in gene associated with the development of intolerant immune response that may be relevant to atherosclerosis. Key genes responsible for the
chronification of inflammation were discovered.
Single-Cell RNA Sequencing Revealing that MMP12+ Macrophages
are Associated with Cancer Liver Metastasis
Authors: Fan Li , Wang Qiang , Qian Qing , Wang Qing , Liu Dan , Gong Yuzhu , Xiong Zhiyong
Published: September 2024

Aim:

We aimed to explore the MMP12+ macrophages function in liver metastasis
of Colorectal cancer [CRC].

Background:

CRC has a high incidence, and a great many patients develop liver metastases.
Some studies have found that macrophages may participate in the liver metastasis
of CRC.

Objective:

This study aimed to determine the factors and major signaling pathways of
MMP12+ macrophages affecting liver metastasis of CRC.

Methods:

The single-cell RNA sequencing [scRNA-seq] data of CRC and bulk transcriptome
data were downloaded. After filtering scRNA-seq data, dimensionality reduction
and clustering were performed to identify different cell subgroups. The FindAll-
Markers function was used to calculate the differentially expressed genes in each cell
subgroup, and the genes in the promising set were uploaded to the DAVID database to
analyze the biological processes to which these genes were enriched. Differentially expressed
genes among macrophage subgroups were selected by the AverageExpression
function. Then, the CIBERSORT algorithm was used to compute the proportion of each
macrophage subgroup in each bulk tissue and determine the most significant
macrophage subgroup. The dynamic changes of gene expression in macrophage subgroup
were computed by Pseudotime. Finally, CellChat was applied to investigate the
effect of the macrophage subgroup on epithelial cells and the ligand-receptor effect of
B cells and T cells.

Results:

Clustering scRNA-seq data showed a larger proportion of macrophages in liver
metastases. The proportion of MMP12+ macrophage subtypes increased gradually
among normal, tumor, and liver metastasis groups, and MMP12+ macrophages were associated
with angiogenesis, cell migration, and inhibited T cell proliferation. The Pseudotime
showed higher expression levels of genes related to angiogenesis and enhanced
TGF-β signaling pathway and the negative regulation of T cell proliferation with the occurrence
of liver metastasis in MMP12+ macrophages. MMP12+ macrophages can promote
the proliferation of epithelial cells and inhibit the activation of T cells and B cells.

Conclusion:

MMP12+ macrophages promoted liver metastasis of CRC by influencing
angiogenesis, TGF-β signaling pathway expression, and regulation of T cells and B
cells.
A Revolutionary Approach for Combating Efflux Transporter-mediated Resistant Epilepsy: Advanced Drug Delivery Systems
Authors: Tonk Megha , Singh Indu , Sharma Ram Jee , Chauhan Shikha Baghel
Published: September 2024

Epilepsy is a persistent neurological condition that affects 60 million individuals globally, with recurrent
spontaneous seizures affecting 80% of patients. Antiepileptic drugs (AEDs) are the main course of therapy
for approximately 65% of epileptic patients, and the remaining 35% develop resistance to medication,
which leads to drug-resistant epilepsy (DRE). DRE continues to be an important challenge in clinical epileptology.
There are several theories that attempt to explain the neurological causes of pharmacoresistance in epilepsy.
The theory that has been studied the most is the transporter hypothesis. Therefore, it is believed that upregulation
of multidrug efflux transporters at the blood-brain barrier (BBB), such as P-glycoprotein (P-gp), which
extrudes AEDs from their target location, is the major cause, leading to pharmacoresistance in epilepsy. The
most effective strategies for managing this DRE are peripheral and central inhibition of P-gp and maintaining
an effective concentration of the drug in the brain parenchyma. Presently, no medicinal product that inhibits Pgp
is being used in clinical practice. In this review, several innovative and promising treatment methods, including
gene therapy, intracranial injections, Pgp inhibitors, nanocarriers, and precision medicine, are discussed.
The primary goal of this work is to review the P-gp transporter, its substrates, and the latest novel treatment
methods for the management of DRE.
Therapeutic Correlation of TLR-4 Mediated NF-κB Inflammatory Pathways in Ischemic Injuries
Authors: Sharma Veerta , Sharma Prateek , Singh Thakur Gurjeet
Published: September 2024

:

Ischemia-reperfusion (I/R) injury refers to the tissue damage that happens when blood
flow returns to tissue after a period of ischemia. I/R injuries are implicated in a large array of
pathological conditions, such as cerebral, myocardial, renal, intestinal, retinal and hepatic ischemia.
The hallmark of these pathologies is excessive inflammation. Toll-like receptors (TLRs)
are recognized as significant contributors to inflammation caused by pathogens and, more recently,
inflammation caused by injury. TLR-4 activation initiates a series of events that results in activation
of nuclear factor kappa-B (NF-κB), which stimulates the production of pro-inflammatory
cytokines and chemokines, exacerbating tissue injury. Therefore, through a comprehensive review
of current research and experimentation, this investigation elucidates the TLRs signalling pathway
and the role of TLR-4/NF-κB in the pathophysiology of I/R injuries. Furthermore, this review
highlights the various pharmacological agents (TLR-4/NF-κB inhibitors) with special emphasis on
the various ischemic injuries (cerebral, myocardial, renal, intestinal, retinal and hepatic). Future research
should prioritise investigating the specific molecular pathways that cause TLR-4/NF-κBmediated
inflammation in ischemic injuries. Additionally, efforts should be made to enhance treatment
approaches in order to enhance patient outcomes.
SELENBP1 Inhibits the Warburg Effect and Tumor Growth by Reducing
the HIF1α Expression in Colorectal Cancer
Authors: Song Tao , Zhang Xiaotian , Ren Jun , Hu Zhiqing , Wang Xin , Niu Gengming
Published: September 2024

Introduction: Colorectal cancer (CRC) is experiencing a significant increase in both
incidence and mortality rates globally. The expression of Selenium-binding protein 1
(SELENBP1) has been reported to be notably downregulated in various malignancies, yet its
biological functions and cellular mechanisms in CRC remain incompletely understood.

Method: In our investigation, we observed the downregulation of SELENBP1 in CRC tissues
through quantitative real-time PCR and western blotting and identified a positive correlation
between higher SELENBP1 expression and improved survival prognosis using Kaplan–Meier
survival analysis. Through loss-of-function and gain-of-function studies, we demonstrated the
tumor-suppressive roles of SELENBP1 in CRC, supported by results from both in vitro and in
vivo experiments. Furthermore, we uncovered the pivotal functions of SELENBP1 in
suppressing aerobic glycolysis in CRC cells by regulating glucose uptake, lactate generation,
and extracellular acidification rate.

Result: At a mechanistic level, we found that SELENBP1
inhibits the expression of the key glycolytic modulator hypoxia-inducible factor 1 subunit
alpha (HIF1α), and the inhibition of glycolysis by SELENBP1 can be reversed by ectopic
expression of HIF1α. Therefore, our study highlights the potential of SELENBP1 as a
promising target for CRC therapy, given its significant impact on tumor suppression and
reprogrammed glucose metabolism.

Conclusion: These findings contribute to a deeper
understanding of the molecular mechanisms underlying CRC progression and may pave the
way for the development of targeted therapies for this challenging disease.
Physical and Functional Properties of Poly (Lactic Acid)/Paprika Oleoresin Films for Food Packaging Application
Authors: Pens Cristian , Venzke Klug Tâmmila , Santiago Tupuna Yerovi Diego , Flôres Simone , de Oliveira Rios Alessandro
Published: September 2024

Background:

Paprika oleoresin is a compound extracted from paprika, with functional
properties, mainly due to its fatty acid composition and carotenoid profile. The effect of these
compounds on PLA composite films has not been investigated.

Objective:

The objective of this study is to evaluate the effect of paprika oleoresin at different
concentrations on the surface color, UV barrier, mechanical barrier, water vapor barrier, hydrophobicity,
paprika oleoresin release and antibacterial properties of PLA-based composite films.

Method:

Functional poly(lactic acid)-based films incorporating paprika oleoresin extract (POE) at
three levels (0.01%, 0.03% and 0.06%) were prepared using a solution casting method.

Results:

The paprika oleoresin showed an excellent compatibility with the PLA, as a uniform
dispersion was observed. Moreover, the addition of POE improved properties such as elasticity
(increase of 62%) and water vapor permeability (decrease of 69%). However, compared to the
control film (polylactic acid), the solubility of the polylactic acid and paprika oleoresin extract
films was found to be significantly increased. As the POE content increased, the color intensity of
the PLA films became lighter orange-yellow. The film composed by PLA/ POE (0.06%) also presented
significant bacterial inhibition for Staphylococcus aureus. In addition, the films demonstrated
total carotenoid release in food-simulating liquids.

Conclusion:

Functional PLA/POE films can be a good alternative to replace traditional food
packaging.
Network Pharmacology Analysis Reveals Multi-target Interactions of Phytochemicals in Cardiospermum halicacabum and their Potential in
Breast Cancer Therapy
Authors: Shanmugarajan Dhivya , David Charles , Parihar Arpana , Kulkarni Pallavi , Arya Neha , Khan Raju
Published: September 2024

Aims:

This present study aims to probe the interrelationships between phytochemical
components and drug targets in Cardiospermum halicacabum.

Background:

Indian traditional medicine, often called Ayurveda, has a rich history of using medicinal
plants for various health remedies and treatments. Network pharmacology plays a significant
role in understanding the complex interactions between bioactive compounds in herbal medicinal
plants and the human body.

Objective:

Medicinal plants are abundant in bioactive compounds, each possessing unique biological
activities. However, certain unexplored biological activities of medicinal plants remain underrepresented
in therapeutic studies. This study seeks to explore the connections between phytochemical
components and drug targets specifically within Cardiospermum halicacabum.

Methods:

Metabolites were associated with various targets, and network analysis was conducted
to assess the gene-metabolite network using cytoHubba. Genes were clustered to understand their
involvement in gene ontology, biological processes, and metabolic pathways. K-means clustering
illustrated a network of genes related to breast cancer pathways. Molecular docking analysis was
performed for common genes within multiple targets to assess docking scores and interactions.

result:

Network analysis revealed six key metabolites closely interacting with multiple targets. Antioxidant activity was assessed using the DPPH assay, demonstrating significant effects (inhibition 74.08 ± 0.67%). Cytotoxicity against MCF-7 and MDA-MBA 231 cell lines showed IC50 values of 110.7 µg/mL and 141.2 µg/ml, respectively, indicating potential cytotoxic effects.

Results:

Network analysis revealed six key metabolites closely interacting with multiple targets.
Antioxidant activity was assessed using the DPPH assay, demonstrating significant effects (inhibition
74.08 ± 0.67%). Cytotoxicity against MCF-7 and MDA-MBA 231 cell lines showed IC50
values of 110.7 μg/mL and 141.2 μg/ml, respectively, indicating potential cytotoxic effects.

Conclusion:

This study highlights the importance of plant metabolites and their interactions with
multiple targets through network pharmacology analysis. It suggests the synergistic potential of
compounds with multiple targets, providing insights into their therapeutic value, particularly in
breast cancer therapy.
Guava in Dentistry: A Potent Transition from Traditional to Modern Medicine in Oral Health
Authors: Sultana Ayesha , Aleemuddin Mohammad , Hussain Md Sadique , Mustafa Mohammed , Maqbool Mudasir
Published: September 2024

Psidium guajava or guava is a plant that contains a variety of bioactive compounds
that have been used to treat oral and dental issues. The plant is unique and traditional, with a wide
range of therapeutic uses. Guava's healing and medicinal effects have been reported through clinical
trials, laboratory studies, and surveys. Over 3.5 billion people worldwide suffer from oral
disorders, such as dental caries, periodontal disease, plague, gum inflammation, toothache, tooth
loss, and oral cancer. The World Health Organization (WHO) advocates for affordable, safe, and
environmentally friendly oral hygiene products to combat the high cost of treatment for oral illnesses.
Guava tree parts, including roots, leaves, bark, stem, and fruits, are used to treat dental
ailments, like halitosis, bleeding gums, gingivitis, periodontal conditions, dental caries, toothache,
and mouth cancers. Phytochemicals, with their properties, have been proven effective in treating
and managing systemic and dental diseases. Guava, a rich source of varied phyto-constituents,
effectively combats oxidative stress, thereby preventing the deterioration of oral health. The
translation of Psidium guajava from traditional medicine to modern medicine can be a boon to
cost-effective and potent treatment in managing oral health and dental disorders.
MBPathNCP: A Metabolic Pathway Prediction Model for Chemicals and Enzymes Based on Network Consistency Projection
Authors: Chen Lei , Hu Huiru
Published: September 2024

Background:

Metabolic pathway is an important biological pathway in living organisms
as it produces necessary energy to maintain vital movement. Although main part of metabolic
pathway has been uncovered by the great efforts in recent years, its completeness is still a problem.
The undetected chemical reactions in metabolic pathway have become a hinder for better understanding
on its mechanism. Prediction of metabolic pathways that a chemical or enzyme can participate
in is the first step to remove this hinder.

Objective:

This study aimed to design an effective computational method to predict the metabolic
pathways of chemicals and enzymes.

Methods:

A new computational model was proposed to predict the metabolic pathways of chemicals
and enzymes, which was called MBPathNCP. The kernels for chemicals/enzymes and pathways
were constructed using the interactions of chemicals and proteins, and the validated associations
between chemicals/enzymes and pathways. The network consistency projection was applied to
the kernels and association adjacency matrix to yield the association score for each pair of chemicals/
enzymes and pathways.

Results:

Cross-validation results on this model shown its good performance. The further tests indicated
the reasonability of the entire architecture and its superiority when the negative samples were
much than positive samples.

Conclusion:

The proposed model MBPathNCP was efficient to predict the metabolic pathways of
chemicals and enzymes and can be a latent useful tool to investigate metabolic pathway system.
Participation of Vietnamese enterprises in the global footwear supply chain
Authors:   School of Business Management, Trade Union University, Hanoi, Vietnam, Oanh Pham Thi , Huong Luc Thi Thu ,   School of International Business and Economics, Thuongmai University, Hanoi, Vietnam, Tran The Tuan ,   Department of Economics Transport, University of Transport Technology, Hanoi, Vietnam
Published: September 2024

This study examines the factors influencing the involvement of Vietnamese enterprises in the global footwear supply chain. It uses the Porter Diamond Model to evaluate the competitive advantages of Vietnamese footwear enterprises based on four key attributes: factor conditions, demand conditions, related and supporting industries, and firm strategy, structure, and rivalry. The research combines various methods, including a review of previous studies, analysis of secondary data, and in-depth interviews with twelve industry experts. The findings offer insights and managerial implications for enhancing supply chain collaboration among Vietnamese footwear companies. It suggests that Vietnamese businesses and policymakers should actively engage in the global supply chain to gain higher competitive advantages and advance to more value-added activities. The study discusses practical and theoretical implications.
ROLE OF INDIVIDUAL MICROELEMENTS AND VITAMINS IN HASHIMOTO'S DISEASE
Authors: Kowalewski Jan , Dąbrowska Aleksandra , Pyc Grzegorz , Bęben Damian , Dudzik Maria , Majewski Kornel , Pakos Kinga , Durmała Kamila , Sielicka Urszula , Mączka Jakub
Published: September 2024

Introduction and objective. Hashimoto's disease is a chronic autoimmune thyroiditis. The chronic process of this disease leads to hypothyroidism. Various micronutrients and vitamins play an important role in the course of the disease and may have a significant impact on the development and treatment of Hashimoto's disease. The aim of this study is to review the literature to fully assess the impact of micronutrients on symptoms, prognosis and dynamics of change in the context of Hashimoto's disease. Articles were searched by entering keywords in PubMed databases. Scientific articles covering the period from 2016 to 2024 represent 76% of the items included in the literature. Studies from specialized research centers including meta-analyses, double-blinded randomized trials, large cross-sectional studies were considered. Studies from inexperienced centers with methodological flaws were discarded.
Abbreviated description of the state of knowledge. Current treatment of clinically overt hypothyroidism is based on the use of levothyroxine. The dose of substitution treatment is determined individually for the patient depending on the symptoms observed, TSH values and age. Currently, there are no recommendations for supplementation of microelements for the disease, which is due to the need to expand research and determine which micronutrients are worth taking to increase the quality of life of patients.
Conclusion. The number of cases of Hashimoto's disease is steadily increasing worldwide. Increasing attention is being paid to elements of the patient's diet and environment, in addition to the substitution treatment itself. The modification of a broadly defined lifestyle and the influence of environmental factors may in the near future find a place in the standard therapeutic management of patients with chronic autoimmune thyroiditis.
DIABETES MELLITUS AND ITS IMPACT ON THE STATE OF THE ORAL CAVITY IN CHILDREN
Authors: Maksymenko Alina , Sheshukova Olga , Polishchuk Tetyana , Trufanova Valentina , Kazakova Kateryna , Bauman Sofia , Pysarenko Olena
Published: September 2024

Today, the number of people with diabetes is growing in all age groups both in our country and around the world. Diabetes is one of the most serious diseases in terms of its course and complications. It is known that diabetes destroys all types of metabolism, changes cellular and humoral immunity, is characterized by persistent hemorrhagic and neuromodulatory disorders, and slows down regenerative and reparative processes. The development of diabetic complications is associated with hyperglycemia and pathological changes in the vascular and peripheral nervous system.
Studies by Ukrainian and foreign scientists have repeatedly shown that the child's body is an integral system and that endocrine disorders are accompanied by changes in various organs and systems, including the dentition. Although periodontal tissues are the main affected organ, other dental lesions, such as caries, inflammatory and infectious diseases of the oral mucosa and salivary glands. They are more common in diabetic patients than in children without somatic lesions. The first and most common symptoms in diabetic patients are dry mouth (xerostomia) and dry lips due to dehydration with polyuria, which is especially noticeable in children with worsening diabetes. Inflammatory periodontal diseases and inflammatory-dystrophic periodontal diseases occupy a special place among the symptoms of diabetes in the oral cavity in diabetic children. Vascular damage is of prime importance in the development of periodontal tissue inflammation in diabetic patients. Microangiopathy in diabetes mellitus enhances the process of bone resorption.
The analysis of scientific material shows that there are significant pathological changes in the oral cavity of children with diabetes mellitus, which increase the dynamics of the disease and worsen its course.
Therefore, the planning of dental treatment of patients with diabetes at each clinical stage should be based on a clear understanding of all etiological relationships, manifestations of various forms of diabetes in the oral cavity and their relationship with the corrected state of carbohydrate metabolism.
A literature search was conducted using the databases Web of Science, Scopus, The Cochrane Library, MedLine, EMBASE and Global Health.
ОБҐРУНТУВАННЯ ЗАСТОСУВАННЯ КАНАГЛІФЛОЗИНУ У ЛІКУВАННІ ХВОРИХ НА ЦУКРОВИЙ ДІАБЕТ
Authors: Романуха Вікторія , Гринів Олена , Нищук-Олійник Наталія , Малініна Наталія
Published: September 2024

Цукровий діабет 2 типу є найважливішою медико-соціальною проблемою сучасності, що обумовлено його значною поширеністю, високим рівнем інвалідизації та смертності. Поширеність цукрового діабету 2 типу у хворих із хронічною серцевою недостатністю має важливе значення внаслідок безпосереднього впливу на перебіг хронічної серцевої недостатності і відповідних змін у традиційних підходах щодо визначення індивідуального ризику несприятливих подій, оптимальної стратегії діагностики та лікування. Крім того, цукровий діабет 2 типу є фактором ризику розвитку хронічної хвороби нирок і її термінальної стадії. Інгібітори натрій-залежного котранспортера глюкози 2 типу (іНЗКТГ-2) є відносно новим класом препаратів, що використовуються для лікування цукрового діабету 2 типу. Застосування іНЗКТГ-2 у повсякденній клінічній практиці викликає інтерес не тільки в ендокринологів, а й у лікарів суміжних спеціальностей. У статті представлено огляд досліджень ефективності та безпеки лікарського засобу з групи іНЗКТГ2 – канагліфлозину. Канагліфлозин є іНЗКТГ-2 з додатковим по відношенню до інших представників цього класу подвійним інсулінонезалежним механізмом дії, пов'язаним з інгібуванням не тільки НЗКТГ-2 нирок, а й НЗКТГ-1 у кишечнику та нирках, що може сприяти кращому контролю глікемії, у тому числі постпрандіальної. У клінічних дослідженнях було показано, що канагліфлозин був ефективним щодо зниження ризику несприятливих серцево-судинних подій, а також знижував ризики, пов'язані із серцевою недостатністю, у пацієнтів із цукровим діабетом 2 типу. Доведені нефропротективні властивості канагліфлозину лягли в основу зареєстрованого показання щодо зниження ризику прогресування ниркових і серцево-судинних наслідків у пацієнтів із цукровим діабетом 2 типу і діабетичною нефропатією. Сприятливий профіль безпеки та переносимості препарату уможливлює його широке застосування у пацієнтів із цукровим діабетом 2 типу. Для наукового огляду використано дані наукометричних баз PubMed, Web of Science, Scopus, Google Scholar тощо та проведено аналіз наукових монографій. Під час відбору 43 праць для написання статті застосовували інформаційно-аналітичний контент-аналіз, обирали останні публікації із досліджуваної проблематики.
O ENSINO DA DANÇA NAS AULAS DE ARTE
Authors: Bezerra da Silva Dorgival , Villegas María Margarita
Published: September 2024

Este artigo apresenta resultados de uma pesquisa de mestrado, realizada entre 2020 e 2022 no Programa de Pós-Graduação em Ensino (PosEnsino), e traz como objetivo analisar as interpretações socioculturais da relação entre corpo, gênero e cultura no processo de constituição dos sujeitos dançarinos do gênero masculino a partir de discussões sobre o ensino da dança na escola. Utilizou-se a abordagem qualitativa, visto a essencialidade do tratamento subjetivo para a realização deste estudo (Villegas; González, 2011), bem como, a perspectiva da fenomenologia e hermenêutica (Minayo, 2008; Medeiros, 2016). Os resultados evidenciam de que a dança se consolida muito mais fortemente nas etapas iniciais da educação básica, enquanto que nas etapas posteriores fica suprimida pela sistematização do ensino. Há, ainda, um processo de negociação entre professor e alunos para a efetivação do trabalho com a dança nas séries finais da educação básica.