bluuebunny/crossref_metadata_2025 huggingface dataset:
Real-Time Ground Aeroservoelastic Test for Slender Vehicles Based on Condensed Aerodynamic Force Loading
Authors: Yu Changkun , Wu Zhigang , Yang Chao
Published: January 2024

Slender vehicles often encounter significant aeroservoelastic challenges due to their low elastic mode frequencies and wide servo control system bandwidths. Traditional analysis methods have limitations, including low modeling accuracy for real vehicles in numerical methods, scale errors in wind tunnel tests, and significant risks in flight tests. The ground aeroelastic stability test is an innovative experimental method designed to address these challenges. This novel method employs shakers to apply condensed unsteady aerodynamic forces in real-time to actual vehicles, serving for both the ground flutter test (GFT) and the ground aeroservoelastic test (GAT). While extensive research exists on the GFT, there is limited exploration of the GAT. For the GAT of a slender vehicle in this paper, the condensed aerodynamic forces are calculated using the quasi-steady aerodynamic derivative method. An improved, partially decoupled inverse model controller is designed for force control, guided by an assessment of coupling strength among different shakers. Ground experiments under various flight control laws and flight dynamic pressures produce accurate results. Numerical simulations and experimental results demonstrate high precision, with excitation force amplitude deviations within ±10% and phase deviations within ±5° within the frequency range relevant to aeroservoelastic stability.
Asynchronously H∞ Tracking Control and Optimization for Switched Flight Vehicles with Time-Varying Delay
Authors: Yang Xing , Fu Bin , Ma Xiaochuan , Liu Yu , Yuan Dongyu , Wu Xintong
Published: January 2024

The current paper verifies the asynchronous H∞ control and optimization problem for flight vehicles with a time-varying delay. The nonlinear dynamic model and Jacobian linearization establish the flight vehicle’s switched model. An asynchronous H∞ tracking controller is designed, considering the existing asynchronous switching between the controllers and corresponding subsystems. In order to promote transient efficiency, the tracking controller comprises the model-based part and the learning-based part. The model-based part guarantees stability and prescribed efficiency, and the learning-based part compensates for undesirable uncertainties. The multiple Lyapunov function (MLF) and mode-dependent average dwell time (MDADT) methods are utilized to guarantee stability and the specified attenuation efficiency. The existing conditions and the solutions of model-based sub-controllers are represented by linear matrix inequalities (LMIs). The deep Q learning (DQL) algorithm provides the learning-based part. Different from the conventional method, the controller parameters are scheduled online. Therefore, robustness, stability, and dynamic efficiency can be met simultaneously. A numerical example illustrates the efficiency and advantage of the presented approach.
Predicting Floral Bud Progression for Three Peach Cultivars
Authors: Cifuentes-Carvajal Adriana , Chaves-Córdoba Bernardo , Vinson Edgar L. , Coneva Elina D. , Chavez Dario J. , Salazar-Gutiérrez Melba R.
Published: January 2024

The prediction of floral bud progression in commercial peach cultivars promotes knowledge about the adaptability of cultivars to the climatic conditions in a specific location under a climatic variability context. Phenology is relevant to improving the scheduling of cultural practices in peach orchards. This research aimed to predict the floral bud progression of three peach cultivars: ‘Harvester’, ‘Red Globe’, and ‘Rubyprince’. Floral bud progression was assessed using one-year-old shoots collected from an orchard located at the Chilton Research and Extension Center, Alabama. Samples were evaluated under laboratory and growth chamber conditions. We recorded the flower developmental stages daily to identify the transition among stages. Daily temperature records were used to estimate the heat requirement in terms of Growing Degree Days (GDD). After dormancy release, samples needed between 23 to 39 and 37 to 42 days to reach the petal fall stage for season 1 and season 2, respectively. In terms of heat requirements, cultivars needed between 425.5 to 721.5 and 684.5 to 777 GDD to end flowering. A logistic curve was adjusted to describe the growth of the different floral stages over time. The results are key to supporting peach growers in crop management practices such as orchard establishment, irrigation, fertilization, freeze and frost protection, and pollination under climate variability scenarios.
Genetic Gains of Grain Yield among the Maize Cultivars Released over a Century from the National Breeding Program of Zimbabwe
Authors: Mazibuko Purity , Mutengwa Charles , Magorokosho Cosmos , Kutywayo Dumisani , Kamutando Casper Nyaradzai
Published: January 2024

Monitoring genetic gain is required in crop improvement programs in order to assess the effectiveness of breeding initiatives. The periodic measurement of genetic gain will quantify the efficiency of new technologies incorporated into the program. Here, a total of 24 cultivars (20 released from Zimbabwe’s National Breeding program (ZNBP) plus 4 released by the largest and oldest private seed company in Zimbabwe, Seed Co) over the period of 1900–2016, were evaluated across 10 locations in Zimbabwe. The testing locations represented agro-ecologies where maize is optimally grown and where maize production is under threat from climate change-induced abiotic stresses, particularly drought and heat stress, in Zimbabwe. The 24 cultivars were laid out across all the locations using the alpha (0.1) lattice design replicated two times with six incomplete blocks nested in each of the replicates. The genetic gains were estimated at 61 kg ha−1 yr−1, 25 kg ha−1 yr−1, 6 kg ha−1 yr−1, and 2 kg ha−1 yr−1 under optimal, random drought stress, heat stress, and managed drought stress conditions, respectively. The results were comparable with those from other studies where newly released cultivars yielded more than old cultivars. Overall, the results demonstrated that over a century, the ZNBP has been making significant progress in yield increments in its maize genotypes.
Predictions and Estimations in Agricultural Production under a Changing Climate
Authors: Niedbała Gniewko , Piekutowska Magdalena , Wojciechowski Tomasz , Niazian Mohsen
Published: January 2024

In the 21st century, agriculture is facing numerous challenges [...]
Integration of Deep Learning and Sparrow Search Algorithms to Optimize Greenhouse Microclimate Prediction for Seedling Environment Suitability
Authors: Shi Dongyuan , Yuan Pan , Liang Longwei , Gao Lutao , Li Ming , Diao Ming
Published: January 2024

The climatic parameters within greenhouse facilities, such as temperature, humidity, and light, exert significant influence on the growth and yield of crops, particularly seedlings. Therefore, it is crucial to establish an accurate predictive model to monitor and adjust the greenhouse microclimate for optimizing the greenhouse environment to the fullest extent. To precisely forecast the greenhouse microclimate and assess the suitability of nursery environments, this study focuses on greenhouse environmental factors. This study leveraged open-source APIs to acquire meteorological data, integrated a model based on Convolutional Neural Networks (CNN) and Long Short-Term Memory Networks (LSTM), and utilized the sparrow search algorithm to optimize model parameters, consequently developing a time series greenhouse microclimate prediction model. Furthermore, Squeeze-and-Excitation (SE) Networks were employed to enhance the model’s attention mechanism, enabling more accurate predictions of environmental factors within the greenhouse. The predictive results indicated that the optimized model achieved high precision in forecasting the greenhouse microclimate, with average errors of 0.540 °C, 0.936%, and 1.586 W/m2 for temperature, humidity, and solar radiation, respectively. The coefficients of determination (R2) reached 0.940, 0.951, and 0.936 for temperature, humidity, and solar radiation, respectively. In comparison to individual CNN or LSTM models, as well as the back-propagation (BP) neural network, the proposed model demonstrates a significant improvement in predictive accuracy. Moreover, this research was applied to the greenhouse nursery environment, demonstrating that the proposed model significantly enhanced the efficiency of greenhouse seedling cultivation and the quality of seedlings. Our study provided an effective approach for optimizing greenhouse environmental control and nursery environment suitability, contributing significantly to achieving sustainable and efficient agricultural production.
Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept
Authors: Leal Reis Inês , Lopes Bruna , Sousa Patrícia , Sousa Ana Catarina , Branquinho Mariana V. , Caseiro Ana Rita , Rêma Alexandra , Briote Inês , Mendonça Carla M. , Santos Jorge Miguel , Atayde Luís M. , Alvites Rui D. , Maurício Ana Colette
Published: January 2024

Horses are high-performance athletes prone to sportive injuries such as tendonitis and desmitis. The formation of fibrous tissue in tendon repair remains a challenge to overcome. This impels regenerative medicine to develop innovative therapies that enhance regeneration, retrieving original tissue properties. Multipotent Mesenchymal Stem/Stromal Cells (MSCs) have been successfully used to develop therapeutic products, as they secrete a variety of bioactive molecules that play a pivotal role in tissue regeneration. These factors are released in culture media for producing a conditioned medium (CM). The aforementioned assumptions led to the formulation of equine synovial membrane MSCs (eSM-MSCs)—the cellular pool that naturally regenerates joint tissue—combined with a medium enriched in immunomodulatory factors (among other bioactive factors) produced by umbilical cord stroma-derived MSCs (eUC-MSCs) that naturally contribute to suppressing the immune rejection in the maternal–fetal barrier. A description of an equine sport horse diagnosed with acute tarsocrural desmitis and treated with this formulation is presented. Ultrasonographic ligament recovery occurred in a reduced time frame, reducing stoppage time and allowing for the horse’s return to unrestricted competition after the completion of a physical rehabilitation program. This study focused on the description of the therapeutic formulation and potential in an equine desmitis treatment using the cells themselves and their secretomes.
Investigating the Impact of Varied Dietary Protein Levels on Litopenaeus vannamei: An Exploration of the Intestinal Microbiota and Transcriptome Responses
Authors: Li Gongyu , Yuan Hang , Fu Zhibin , Luo Xinghui , Xue Zhihao , Zhang Shuang
Published: January 2024

This study explored the effects of dietary protein levels on Litopenaeus vannamei with its intestinal microbiota and transcriptome responses. Previous studies on the effects of dietary protein levels on L. vannamei have focused on growth performance, antioxidant indices, and digestive enzyme activity, but few studies have been conducted at the microbiological and molecular levels. In this study, five isolipid experimental diets with protein levels of 32% (P32), 36% (P36), 40% (P40), 44% (P44), and 48% (P48) were used in an L. vannamei (0.63 ± 0.02 g) feeding trial for 56 days. At the end of the feeding trial, the growth performance, immunity, intestinal health, and transcriptional responses of L. vannamei were determined. This study demonstrated that higher protein levels (P44) led to superior weight gain and growth rates for L. vannamei, with lower feed conversion ratios (FCR) observed in the P48 and P44 groups compared to the P32 and P36 groups (p ≤ 0.05). The P44 and P48 groups also showed a notably higher protein efficiency ratio (PER) compared to others (p ≤ 0.05), and there was no significant difference between them. Upon Vibrio parahaemolyticus infection, the P48 group exhibited a significantly lower survival rate (SR) within 48 h, while during 72 h of white spot syndrome virus (WSSV) infection, the P44 group had a notably higher survival rate than the P32 group (p ≤ 0.05). Digestive enzyme activity and antioxidant levels in L. vannamei initially increased and then decreased as protein levels increased, usually peaking in the P40 or P44 groups. Lower dietary protein levels significantly reduced the relative abundance of beneficial bacteria and increased the relative abundance of pathogenic bacteria in the intestines of L. vannamei. Transcriptome sequencing analysis revealed that most differentially expressed genes (DEGs) were up-regulated and then down-regulated as dietary protein levels increased. Furthermore, KEGG pathway enrichment analysis indicated that several immune and metabolic pathways, including metabolic pathways, glutathione metabolism, cytochrome P450, and lysosome and pancreatic secretion, were significantly enriched. In summary, the optimal feed protein level for L. vannamei shrimp was 40–44%. Inappropriate feed protein levels reduced antioxidant levels and digestive enzyme activity and promoted pathogen settlement, deceasing factors in various metabolic pathways that respond to microorganisms through transcriptional regulation. This could lead to stunted growth in L. vannamei and compromise their immune function.
The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in Cyprinus carpio L.
Authors: Grădinariu Lăcrămioara , Dediu Lorena , Crețu Mirela , Grecu Iulia Rodica , Docan Angelica , Istrati Daniela Ionela , Dima Floricel Maricel , Stroe Maria Desimira , Vizireanu Camelia
Published: January 2024

Berberine (BBR) and silymarin (SM) are natural compounds extracted from plants known for their antioxidant and chemoprotective effects on the liver. The present study aimed to investigate the beneficial properties of BBR and SM and the association of BBR with SM on liver function using fish as “in vivo” models. Moreover, the study investigated their hepatoprotective role after acetaminophen (APAP) exposure. For this purpose, the fish (N = 360; 118.4 ± 11.09 g) were fed with control or experimental diets for 9 weeks. In the experimental diets, the feed was supplemented with either SM (1 g/kg feed), BBR (100 and 200 mg/kg feed), or a combination of BBR with SM (SM 1 g/kg feed + BBR 100 mg/kg feed and, respectively, SM 1 g/kg feed + BBR 200 mg/kg feed). After the feeding trial, seven fish from each tank were randomly selected and exposed to a single APAP dose. The selected serum biochemical markers, oxidative stress markers, and lysozyme activity were used to evaluate the efficiency of the supplements on carp’s health profile, particularly regarding the hepatopancreas function. Our results showed that the inclusion of SM and BBR (either as a single or in combination) reduced the serum contents of total cholesterol, triglyceride, and alanine transaminase. An increase in the high-density cholesterol was observed after the administration of BBR or BBR in association with SM. Both supplements showed hepatoprotective activity against APAP-induced hepatotoxicity, especially BBR. The ameliorative effects of SM (1 g) in association with BBR (100 mg) were highlighted by the modulation of the nonspecific immune system and oxidative stress alleviation after APAP exposure.
The Yield of One vs. Two Blood Cultures in Children: Under-Detection and Over-Testing
Authors: Zalmanovich Anat , Temkin Elizabeth , Biran Dikla , Carmeli Yehuda
Published: January 2024

We aimed to determine whether obtaining two blood cultures (BCs) instead of one improved the detection of bloodstream infections (BSIs) in children. For this descriptive study, we used surveillance data collected in 2019–2021 from all Israeli hospitals serving children. The sample included 178,702 culturing episodes. One BC was taken in 90.1% of all episodes and 98.2% of episodes in the emergency department. A true pathogen was detected in 1687/160,964 (1.0%) of single-culture episodes and 1567/17,738 (8.9%) of two-culture episodes (p < 0.001). The yield was significantly different even when considering only the first BC in two-culture episodes: 1.0% vs. 7.5%. Among 1576 two-culture episodes that were positive for a true pathogen, the pathogen was detected only in the second culture in 252 (16.0%). We estimated that if a second culture had been taken in all episodes, an additional 343 BSIs by a true pathogen would have been detected. Among 1086 two-culture episodes with commensal bacteria, the second BC was sterile in 530 (48.8%), suggesting contamination. A commensal was isolated in 3094/4781 (64.7%) positive single-culture episodes, which could represent BSI or contamination. The yield of a single BC bottle was low, reflecting both lower sensitivity of a single bottle and the taking of single bottles in patients with a low probability of BSI.
The Design and Control of a Biomimetic Binocular Cooperative Perception System Inspired by the Eye Gaze Mechanism
Authors: Qin Xufang , Xia Xiaohua , Ge Zhaokai , Liu Yanhao , Yue Pengju
Published: January 2024

Research on systems that imitate the gaze function of human eyes is valuable for the development of humanoid eye intelligent perception. However, the existing systems have some limitations, including the redundancy of servo motors, a lack of camera position adjustment components, and the absence of interest-point-driven binocular cooperative motion-control strategies. In response to these challenges, a novel biomimetic binocular cooperative perception system (BBCPS) was designed and its control was realized. Inspired by the gaze mechanism of human eyes, we designed a simple and flexible biomimetic binocular cooperative perception device (BBCPD). Based on a dynamic analysis, the BBCPD was assembled according to the principle of symmetrical distribution around the center. This enhances braking performance and reduces operating energy consumption, as evidenced by the simulation results. Moreover, we crafted an initial position calibration technique that allows for the calibration and adjustment of the camera pose and servo motor zero-position, to ensure that the state of the BBCPD matches the subsequent control method. Following this, a control method for the BBCPS was developed, combining interest point detection with a motion-control strategy. Specifically, we propose a binocular interest-point extraction method based on frequency-tuned and template-matching algorithms for perceiving interest points. To move an interest point to a principal point, we present a binocular cooperative motion-control strategy. The rotation angles of servo motors were calculated based on the pixel difference between the principal point and the interest point, and PID-controlled servo motors were driven in parallel. Finally, real experiments validated the control performance of the BBCPS, demonstrating that the gaze error was less than three pixels.
Additive Manufacturing Applications in Biosensors Technologies
Authors: Paul Abraham Abbey , Aladese Adedamola D. , Marks Robert S.
Published: January 2024

Three-dimensional (3D) printing technology, also known as additive manufacturing (AM), has emerged as an attractive state-of-the-art tool for precisely fabricating functional materials with complex geometries, championing several advancements in tissue engineering, regenerative medicine, and therapeutics. However, this technology has an untapped potential for biotechnological applications, such as sensor and biosensor development. By exploring these avenues, the scope of 3D printing technology can be expanded and pave the way for groundbreaking innovations in the biotechnology field. Indeed, new printing materials and printers would offer new possibilities for seamlessly incorporating biological functionalities within the growing 3D scaffolds. Herein, we review the additive manufacturing applications in biosensor technologies with a particular emphasis on extrusion-based 3D printing modalities. We highlight the application of natural, synthetic, and composite biomaterials as 3D-printed soft hydrogels. Emphasis is placed on the approach by which the sensing molecules are introduced during the fabrication process. Finally, future perspectives are provided.
Prospective Mental Images: A Transdiagnostic Approach to Negative Affectivity and Mood Dysregulation among Borderline Personality Disorder and Depression
Authors: Kroener Julia , Schaitz Caroline , Sosic-Vasic Zrinka
Published: January 2024

There is initial evidence that patients diagnosed with Borderline Personality Disorder (BPD) experience intrusive prospective mental images about non-suicidal self-injury (NSSI). These images, in turn, are associated with the conduct of NSSI. As the negative emotional valence of intrusive images has been established across clinical disorders, negative affectivity might play a key role linking mental imagery and psychopathology. Therefore, the present study aimed to investigate the possible mediating role of symptoms of depression as a proxy for negative affectivity linking intrusive prospective imagery to psychopathology in patients diagnosed with BPD. A total of 233 participants (84 diagnosed with MDD, 66 diagnosed with BPD, 83 healthy controls) completed questionnaires on negative affectivity (BDI-II) and prospective intrusive imagery (IFES-S). Before controlling for negative affectivity, there was a positive correlation between group and intrusive prospective imagery, indicating that healthy participants displayed lower amounts of intrusive prospective images in comparison to patients diagnosed with MDD or BPD. After entering negative affectivity as a mediator, the variable group was no longer associated with intrusive prospective images; however, negative affectivity showed a strong and positive relationship with the group on one side, and intrusive prospective imagery on the other, indicating that negative affectivity mediates the association between intrusive prospective images and clinical disorders. The presented findings point towards a mediating role of negative affectivity in the manifestation of intrusive prospective imagery, not only within BPD, but also in patients with MDD. The possibility of intrusive images acting as a transdiagnostic feature, where negative affectivity and mood dysregulation are at the core of the clinical disorder, are being discussed.
The Relationship between Childhood Abuse and Suicidal Ideation among Chinese College Students: The Mediating Role of Core Self-Evaluation and Negative Emotions
Authors: Pan Zhaoxia , Zhang Dajun , Bian Xiaohua , Li Hongye
Published: January 2024

Childhood abuse is a significant risk factor for suicidal ideation. However, the underlying mediation mechanism necessitates further exploration. This study investigated the mediating role of core self-evaluation and negative emotions in the relationship between childhood abuse and suicide ideation in young adults. A sample of 3103 college students from 11 universities across 8 provinces in China was analyzed. Childhood abuse, core self-evaluation, negative emotions, and suicidal ideation were assessed using the Childhood Trauma Questionnaire (CTQ-CF), Core Self-Evaluation Scale, Affect Scale, and Beck Suicidal Ideation Scale (BSI-CV), respectively. Data analysis was conducted using SPSS 19.0 and SPSS Macro Process. We found that high scores for childhood abuse were associated with elevated levels of suicidal ideation, whereas low scores for core self-evaluation were closely linked to heightened levels of negative emotions and suicidal ideation. Furthermore, core self-evaluation and negative emotions mediated the relationship between childhood abuse and suicidal ideation through three significant paths. The results demonstrate that childhood abuse can directly impact suicidal ideation in young adulthood and indirectly influence suicidal ideation by affecting core self-evaluation and negative emotions. They suggest that addressing core self-evaluation and negative emotions in individuals who have experienced childhood abuse may help prevent or treat suicidal ideation.
Clinical Potential of YY1-Hypoxia Axis for Vascular Normalization and to Improve Immunotherapy
Authors: Meo Concetta , de Nigris Filomena
Published: January 2024

Abnormal vasculature in solid tumors causes poor blood perfusion, hypoxia, low pH, and immune evasion. It also shapes the tumor microenvironment and affects response to immunotherapy. The combination of antiangiogenic therapy and immunotherapy has emerged as a promising approach to normalize vasculature and unlock the full potential of immunotherapy. However, the unpredictable and redundant mechanisms of vascularization and immune suppression triggered by tumor-specific hypoxic microenvironments indicate that such combination therapies need to be further evaluated to improve patient outcomes. Here, we provide an overview of the interplay between tumor angiogenesis and immune modulation and review the function and mechanism of the YY1-HIF axis that regulates the vascular and immune tumor microenvironment. Furthermore, we discuss the potential of targeting YY1 and other strategies, such as nanocarrier delivery systems and engineered immune cells (CAR-T), to normalize tumor vascularization and re-establish an immune-permissive microenvironment to enhance the efficacy of cancer therapy.
SOX2 Expression Does Not Guarantee Cancer Stem Cell-like Characteristics in Lung Adenocarcinoma
Authors: Bae Seung-Hyun , Lee Kyung Yong , Han Suji , Yun Chul Won , Park ChanHyeok , Jang Hyonchol
Published: January 2024

Effectively targeting cancer stemness is essential for successful cancer therapy. Recent studies have revealed that SOX2, a pluripotent stem cell factor, significantly contributes to cancer stem cell (CSC)-like characteristics closely associated with cancer malignancy. However, its contradictory impact on patient survival in specific cancer types, including lung adenocarcinoma (LUAD), underscores the need for more comprehensive research to clarify its functional effect on cancer stemness. In this study, we demonstrate that SOX2 is not universally required for the regulation of CSC-like properties in LUAD. We generated SOX2 knockouts in A549, H358, and HCC827 LUAD cells using the CRISPR/Cas9 system. Our results reveal unchanged CSC characteristics, including sustained proliferation, tumor sphere formation, invasion, migration, and therapy resistance, compared to normal cells. Conversely, SOX2 knockdown using conditional shRNA targeting SOX2, significantly reduced CSC traits. However, these loss-of-function effects were not rescued by SOX2 resistant to shRNA, underscoring the potential for SOX2 protein level-independent results in prior siRNA- or shRNA-based research. Ultimately, our findings demonstrate that SOX2 is not absolutely essential in LUAD cancer cells. This emphasizes the necessity of considering cancer subtype-dependent and context-dependent factors when targeting SOX2 overexpression as a potential therapeutic vulnerability in diverse cancers.
Ensuring Optimal Outcomes for Preterm Infants after NICU Discharge: A Life Course Health Development Approach to High-Risk Infant Follow-Up
Authors: Litt Jonathan S. , Halfon Neal , Msall Michael E. , Russ Shirley Ann , Hintz Susan R.
Published: January 2024

Children born prematurely (<37 weeks’ gestation) have an increased risk for chronic health problems and developmental challenges compared to their term-born peers. The threats to health and development posed by prematurity, the unintended effects of life-sustaining neonatal intensive care, the associated neonatal morbidities, and the profound stressors to families affect well-being during infancy, childhood, adolescence, and beyond. Specialized clinical programs provide medical and developmental follow-up care for preterm infants after hospital discharge. High-risk infant follow-up, like most post-discharge health services, has many shortcomings, including unclear goals, inadequate support for infants, parents, and families, fragmented service provisions, poor coordination among providers, and an artificially foreshortened time horizon. There are well-documented inequities in care access and delivery. We propose applying a life course health development framework to clinical follow-up for children born prematurely that is contextually appropriate, developmentally responsive, and equitably deployed. The concepts of health development, unfolding, complexity, timing, plasticity, thriving, and harmony can be mapped to key components of follow-up care delivery to address pressing health challenges. This new approach envisions a more effective version of clinical follow-up to support the best possible functional outcomes and the opportunity for every premature infant to thrive within their family and community environments over their life course.
Non-Cariogenic Effect of Milk and Dairy Products on Oral Health in Children and Adolescents: A Scoping Review
Authors: Vitiello Flavia , Bourgeois Denis , Orilisi Giulia , Orsini Giovanna , Carrouel Florence
Published: January 2024

Diet plays an important role in the etiopathology of dental caries. Milk and dairy products (DPs), especially in children and adolescents, are important sources of protein, calcium and phosphorus and could have an effect on dental and oral health. The aim of this scoping review was to analyze the scientific literature on the non-cariogenic effect of milk and DPs, with a focus on their potential to prevent dental caries in children and adolescents. PubMed, EMBASE, and Web of Science were searched for publications relevant to our topic from January 2013 to 30 September 2023. Thirty-eight studies were included in the qualitative analysis. The included studies highlight the properties of milk and DPs that contribute to enamel remineralization, exhibit antibacterial action, inhibit the growth of cariogenic bacteria, and promote a balanced oral microbiome. With regard to the addition of probiotics (PBs) and fluoride (F) to DPs, the mixed results of the studies analyzed did not allow a clear statement to be made about their non-cariogenic effects. However, several studies show that the addition of PBs can reduce cariogenic bacteria, create a protective barrier against pathogens and support the host’s natural defense mechanisms. Further long-term and high-quality studies are needed to understand the impact of milk and its constituents on oral health in order to promote effective caries prevention strategies in children and adolescents.
Plasmon-Enhanced High-Order Harmonic Generation of Open-Ended Finite-Sized Carbon Nanotubes with Vacancy Defects
Authors: Hu Qi , Yang Kun , Li Qiuju , Sun Jin , Ding Zongling
Published: January 2024

In this study, the plasmon-enhanced high-order harmonic generation (HHG) of H-terminated finite-sized armchair single-walled carbon nanotubes (SWCNTs) near Ag nanoparticles is investigated systematically. Multiscale methods that combine the real-time time-dependent Hartree–Fock (TDHF) approach at the semi-empirical intermediate neglected differential overlap (INDOS) Hamiltonian level for molecular electronic dynamics with the finite-difference time-domain (FDTD) and solving Maxwell’s equations are used. It is found that for intact CNTs, HHG is significantly enhanced due to plasmon resonance. However, the nonlinear optical properties are saturated when the tube length increases enough in the inhomogeneous near-field. For long CNTs, the large gradient of a near-field is unfavorable for the nonlinear excitation of electrons. But defects can further change the properties of the spectra. The HHG of hybrid systems can be enhanced very clearly by introducing vacancy defects in CNTs. This enhancement is affected by the energy and intensity of the incident light, the near-field gradient, and the number and location of defects.
The Many Unknowns of Partial Sensory Disconnection during Sleep: A Review of the Literature
Authors: Cirelli Chiara , Tononi Giulio
Published: January 2024

When we are asleep, we lose the ability to promptly respond to external stimuli, and yet we spend many hours every day in this inherently risky behavioral state. This simple fact strongly suggests that sleep must serve essential functions that rely on the brain going offline, on a daily basis, and for long periods of time. If these functions did not require partial sensory disconnection, it would be difficult to explain why they are not performed during waking. Paradoxically, despite its central role in defining sleep and what sleep does, sensory disconnection during sleep remains a mystery. We have a limited understanding of how it is implemented along the sensory pathways; we do not know whether the same mechanisms apply to all sensory modalities, nor do we know to what extent these mechanisms are shared between non-rapid eye movement (NREM) sleep and REM sleep. The main goal of this contribution is to review some knowns and unknowns about sensory disconnection during sleep as a first step to fill this gap.
Genome-Wide Identification of the IQM Gene Family and Their Transcriptional Responses to Abiotic Stresses in Kiwifruit (Actinidia eriantha)
Authors: Xu Minyan , Zhang Zhi , Ling Chengcheng , Jiao Yuhuan , Zhang Xin
Published: January 2024

IQM is a plant-specific calcium-binding protein that plays a pivotal role in various aspects of plant growth response to stressors. We investigated the IQM gene family and its expression patterns under diverse abiotic stresses and conducted a comprehensive analysis and characterization of the AeIQMs, including protein structure, genomic location, phylogenetic relationships, gene expression profiles, salt tolerance, and expression patterns of this gene family under different abiotic stresses. Based on phylogenetic analysis, these 10 AeIQMs were classified into three distinct subfamilies (I–III). Analysis of the protein motifs revealed a considerable level of conservation among these AeIQM proteins within their respective subfamilies in kiwifruit. The genomic distribution of the 10 AeIQM genes spanned across eight chromosomes, where four pairs of IQM gene duplicates were associated with segmental duplication events. qRT-PCR analysis revealed diverse expression patterns of these AeIQM genes under different hormone treatments, and most AeIQMs showed inducibility by salt stress. Further investigations indicated that overexpression of AeIQMs in yeast significantly enhanced salt tolerance. These findings suggest that AeIQM genes might be involved in hormonal signal transduction and response to abiotic stress in Actinidia eriantha. In summary, this study provides valuable insights into the physiological functions of IQMs in kiwifruit.
Decoding the Genomic Landscape of Pomegranate: A Genome-Wide Analysis of Transposable Elements and Their Structural Proximity to Functional Genes
Authors: Simoni Samuel , Usai Gabriele , Vangelisti Alberto , Castellacci Marco , Giordani Tommaso , Natali Lucia , Mascagni Flavia , Cavallini Andrea
Published: January 2024

Transposable elements (TEs) significantly drive dynamic changes that characterize genome evolution. However, understanding the variability associated with TE insertions among different cultivars remains challenging. The pomegranate (Punica granatum L.) has yet to be extensively studied regarding the roles of TEs in the diversification of cultivars. Herein, we explored the genome distribution of TEs and its potential functional implications among four pomegranate cultivars, ‘Bhagwa’, ‘Dabenzi’, ‘Taishanhong’ and ‘Tunisia’, whose genome sequences are available. A total of 8404 full-length TEs were isolated. The content of TEs varied among the cultivars, ranging from 41.67% of ‘Taishanhong’ to 52.45% of ‘Bhagwa’. In all cultivars, the Gypsy superfamily of retrotransposons accounted for a larger genome proportion than the Copia superfamily. Seventy-three full-length TEs were found at the same genomic loci in all four cultivars. By contrast, 947, 297, 311, and 874 TEs were found exclusively in ‘Bhagwa’, ‘Dabenzi’, ‘Taishanhong’, and ‘Tunisia’ cultivars, respectively. Phylogenetic clustering based on the presence of TE insertions in specific loci reflected the geographic origins of the cultivars. The insertion time profiles of LTR-REs were studied in the four cultivars. Shared elements across the four cultivars exhibited, on average, a more ancient insertion date than those exclusive to three, two, or one cultivars. The majority of TEs were located within 1000 bp from the nearest gene. This localization was observed for 57% of DNA TEs and 55% of long-terminal repeat retrotransposons (LTR-RE). More than 10% of TEs resulted inserted within genes. Concerning DNA TEs, 3.91% of insertions occurred in introns, while 2.42% occurred in exons. As to LTR-REs, 4% of insertions occurred in exons and 1.98% in introns. Functional analysis of the genes lying close to TEs was performed to infer if differences in TE insertion can affect the fruit quality. Two TE insertions were found close to two genes encoding 4-coumarate--CoA ligase, an enzyme involved in the phenylpropanoid pathway. Moreover, a TIR/Mariner element was found within the exon of a gene encoding anthocyanidin reductase in the ‘Tunisia’ genotype, crucial in the biosynthesis of flavan-3-ols and proanthocyanidins, strictly correlated with the nutraceutical properties of pomegranate. Although functional and metabolomic studies are essential to elucidate the consequences of TE insertions, these results contribute to advancing our comprehension of the role of TEs in pomegranate genomics, providing insights for crop breeding.
A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements
Authors: Lu Xuqiang , He Nan , Anees Muhammad , Yang Dongdong , Kong Weikang , Zhang Jiwen , Yuan Luming , Luo Xiaowen , Zhu Hongju , Liu Wenge
Published: January 2024

Watermelon fruits of different ploidy levels exhibit significant variations in texture. This study aimed to investigate the primary factors that influence texture differences. To achieve this, we conducted an investigation into the differences in fruit texture development using homozygous autogamous diploid (2×) lines and their autotriploid (3×) and autotetraploid (4×) lines ‘Yixuan’. The study investigated fruit development, flesh texture profile analysis (TPA), flesh cell wall polysaccharide content, and flesh cell microstructure analysis in 2×, 3×, and 4× watermelon fruits. The study found that as watermelon fruits matured, several characteristics increased, including fruit weight, TSS (total soluble solids) content, rind hardness, flesh cell size, and cell wall polysaccharide contents such as crude fiber, cellulose, hemicellulose, lignin, and protopectin. In contrast, the flesh texture parameters and soluble pectin content of the flesh cell wall decreased over time. Significant differences in fruit flesh texture and flesh cell structure were observed among fruits of different ploidy levels at the 32 DAP. Specifically, the 2× fruits displayed considerably lower rind hardness, reduced flesh texture (Hardness, Fracturability, Chewiness, Gumminess), and cell density compared to the 3× and 4× fruits. Additionally, the 2× fruits had larger cell sizes than the 3× and 4× fruits. However, there were no significant differences observed in the flesh cell wall polysaccharide contents across various ploidy levels. These findings suggest that the variation in texture among watermelon fruits of different ploidy levels can be attributed to the size and arrangement of the flesh cells. This research provides a foundation for the further exploration of the intrinsic regulatory factors and molecular mechanisms contributing to texture variation in polyploid watermelon fruits.
Green Synthesis of Gold and Copper Nanoparticles by Lannea discolor: Characterization and Antibacterial Activity
Authors: Rambau Unarine , Masevhe Nndivhaleni Anox , Samie Amidou
Published: January 2024

Green synthesis using plant extracts has emerged as an eco-friendly, clean, and viable alternative to chemical and physical approaches. Herein, the leaf, stem, and root extracts of Lannea discolor were utilized as a reducing and stabilizing agent in synthesizing gold (AuNPs) and copper (CuNPs) nanoparticles. The formation of AuNPs and CuNPs, confirmed by their color change, was characterized by UV-Vis spectroscopy (UV-Vis), scanning electron microscopy analysis, and energy-dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), coupled with minimum inhibitory concentration (MIC) antibacterial assays. Gold nanoflowers (AuNFs), NPs, and CuNPs peaked at wavelengths of 316, 544, and 564 nm, respectively. TEM showed unexpected nanoflowers (30–97 nm) in the leaf extracts and spherical NPs (10–33 nm; 9.3–37.5) from stem and root extracts, while spherical CuNPs (20–104 nm) were observed from all the extracts. EDX confirmed the presence of metal salts, and FTIR revealed stable capping agents. AuNPs and NFs from L. discolor extracts showed appreciable antibacterial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), and Bacillus subtilis (ATCC 6633) when compared to the plant extracts. At the same time, none was observed from the CuNPs. These AuNPs and CuNPs are particularly appealing in various biomedical and conductivity manufacturing applications due to their shapes and sizes and economical and environmentally friendly production. To our knowledge, this is the first study of the synthesis of gold and copper nanoparticles from L. discolor.
Agro-Food Waste Valorization for Sustainable Bio-Based Packaging
Authors: Carnaval Luana de S. C. , Jaiswal Amit K. , Jaiswal Swarna
Published: January 2024

In recent years, the increase in the generation of agro-food processing waste, coupled with uncontrolled disposal and inefficient recovery methods, has raised concerns among society, industries, and the research community. This issue is compounded by the accumulation of conventional synthetic packaging. Owing to their significant environmental and economic impacts, the development of sustainable, biocompatible, and biodegradable materials has become an urgent target. In this context, research efforts have been directed toward developing new packaging materials based on renewable sources, such as agro-food waste, contributing to the circular economy concept. However, despite significant advances, novel agro-food-waste-based packaging solutions still largely remain at a laboratory scale. This situation highlights the urgent need for further understanding and thorough investigation into how to upscale these products, thereby promoting engagement, investment, and awareness across various fields. This review aims to discuss the current advances in food packaging development using agro-food waste. It covers the main agro-food wastes and by-products currently recovered for sustainable packaging systems through various approaches, such as the extraction of valuable compounds or waste treatments for incorporation into packaging materials, techniques for their valorization, and recent applications of agro-food waste materials in films and coatings. It also addresses the toxicological and safety approaches, challenges, and future perspectives. After an extensive review, we conclude that current research faces challenges in transitioning novel findings to commercial scale, primarily due to safety factors, high production costs, performance deficits, legislative ambiguities, lack of consumer awareness, and inadequate governmental regulations. Consequently, significant investments in research and development appear to be mandatory in the coming years, aiming for optimized, safe, and cost-effective solutions.
Synthesis of Silver Nanoparticles Using Green Reducing Agent: Ceylon Olive (Elaeocarpus serratus): Characterization and Investigating Their Antimicrobial Properties
Authors: Fernando Kumudu M. , Gunathilake Chamila A. , Yalegama Chandi , Samarakoon Upeka K. , Fernando Chacrawarthige A. N. , Weerasinghe Gangani , Pamunuwa Geethi K. , Soliman Ibrahim , Ghulamullah Nomi , Rajapaksha Suranga M. , Fatani Omar
Published: January 2024

Silver nanoparticles (AgNPs) are widely recognized as a prominent antimicrobial agent and have found applications in the field of medicine. This study focuses on the synthesis of AgNPs utilizing the natural reducing agent of Ceylon olive (Elaeocarpus serratus), presenting an economically viable and ecologically friendly approach. For the first time, this research demonstrated the synthesis of AgNPs using phytochemicals extracted from Ceylon olive, serving as both natural reducing and stabilizing agents. The synthesized AgNPs were characterized with UV–visible spectroscopy, a particle size analyzer (PSA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) coupled with an energy dispersive X-ray spectrometer (EDX). The UV–visible spectra primarily indicated the formation of the AgNPs by the surface plasmon resonance band around 434 nm. SEM analysis confirmed the presence of silver nanoparticles within a size range of 50–110 nm, with an average size of approximately 70 nm. FTIR determined that proteins, phenols, and flavonoids may have acted as reducing and capping agents. Experimental parameters were optimized to improve the yield and size of the AgNPs and eventually evaluate their antibacterial properties. The well diffusion method exhibits a significantly larger zone of inhibition for Gram-negative bacterial strains (18.4 ± 0.55 mm for Pseudomonas aeruginosa and 14.4 ± 0.55 mm for Escherichia coli) compared to Gram-positive bacterial strains (11.6 ± 0.55 mm for Staphylococcus aureus and 10.4 ± 0.55 mm for Staphylococcus epidermidis) for 50 µg/mL AgNPs. These findings demonstrate that AgNPs synthesized with Ceylon olive have the potential to develop into novel materials for bacterial-mediated diseases.
Diversity of Mycorrhizal Fungi in Temperate Orchid Species: Comparison of Culture-Dependent and Culture-Independent Methods
Authors: Mennicken Sophie , Paula Caio César Pires de , Vogt-Schilb Hélène , Jersáková Jana
Published: January 2024

Many orchid species are endangered due to anthropogenic pressures such as habitat destruction and overharvesting, meanwhile, all orchids rely on orchid mycorrhizal fungi (OMF) for seed germination and seedling growth. Therefore, a better understanding of this intimate association is crucial for orchid conservation. Isolation and identification of OMF remain challenging as many fungi are unculturable. In our study, we tested the efficiency of both culture-dependent and culture-independent methods to describe OMF diversity in multiple temperate orchids and assessed any phylogenetic patterns in cultivability. The culture-dependent method involved the cultivation and identification of single pelotons (intracellular hyphal coils), while the culture-independent method used next-generation sequencing (NGS) to identify root-associated fungal communities. We found that most orchid species were associated with multiple fungi, and the orchid host had a greater impact than locality on the variability in fungal communities. The culture-independent method revealed greater fungal diversity than the culture-dependent one, but despite the lower detection, the isolated fungal strains were the most abundant OMF in adult roots. Additionally, the abundance of NGS reads of cultured OTUs was correlated with the extent of mycorrhizal root colonization in orchid plants. Finally, this limited-scale study tentatively suggests that the cultivability character of OMF may be randomly distributed along the phylogenetic trees of the rhizoctonian families.