bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative SWOT Analysis on Factors Influencing the Sustainable Development of Non-Academic Education in China’s Open Universities: A Case Study of Beijing Open University
Authors: Yu Shuyang , Wang Dan
Published: October 2022

In 2012, considering the needs of citizens’ livelihoods and social development, the Chinese government approved six large-scale radio and television universities to become open universities (OUs). Developing non-academic education (NE) has become an important target of OUs’ transformation and development due to its importance in constructing a lifelong learning system in China. To promote the development of NE requires planning a good strategy by analyzing its internal and external environment. The purpose of this research is to provide a referential strategy for OUs in China to develop NE in their transition period. This study used the strengths, weaknesses, opportunities and threats approach in combination with the analytical hierarchy process (SWOT-AHP) to investigate the factors influencing the development of NE in China’s OUs using the example of Beijing Open University. As shown by the research results, although the development of NE in OUs has disadvantages such as the shortage of professional personnel, insufficient capital investment, and poor market operation, the favorable national policy environment, the adjustment of local functional structure, and the extensive learning needs of citizens have led to good external opportunities. Therefore, reverse strategies should be adopted.
Study on the Evolution Law of Surface Landscape Pattern in Earthquake-Stricken Areas by Remote Sensing: A Case Study of Jiuzhaigou County, Sichuan Province
Authors: Bian Zongpan , Zhang Dongdong , Xu Jun , Tang Hongtao , Bai Zhuoli , Li Yan
Published: October 2022

Earthquakes not only cause the destruction of surface buildings and a large number of casualties, but also have an important impact on regional land-use change. Timely understanding of land cover and its changes before and after earthquakes is of great scientific significance for studying the restoration and reconstruction of the natural ecosystem and the sustainable development of the social economy in disaster areas. At present, there are few studies on land cover changes before and after earthquakes in the earthquake-stricken areas, especially the quantitative assessment of land cover changes before and after earthquakes at the micro scale of landscape spatial distribution. Therefore, this article is based on remote sensing data and uses an earthquake in Jiuzhaigou, Sichuan, as an example. By calculating the land-use degree comprehensive index and its rate of change, the transfer matrix of land-use, quantitative expression methods of the landscape via the landscape pattern index are analyzed from the points of view of the spatial–temporal evolution law of the landscape pattern of land cover before and after the earthquake. The experimental results showed that the area of forest, cropland, shrub. and grassland types in the study area changed greatly, and the area of grassland types in Shuanghe Township changed most obviously, as they increased from 6.57 km2 in 2015 to 7.43 km2, and then decreased to 6.21 km2 in 2020. During the earthquake recovery period, the comprehensive index of land-use degree for most towns in the study area showed an upward trend, and the land cover types were improved. After 2017, the PD and LSI of the study area showed a downward trend, among which the bare land type changed greatly, with the PD value decreasing from 0.38 in 2017 to 0.21 in 2020, and the LSI value decreasing from 58.29 in 2017 to 40.69 in 2020. This indicates that the degree of landscape fragmentation and the spatial heterogeneity of the land surface in the study area are reduced in the later stage of earthquake recovery. After 2017, the AI value of the study area showed an upward trend, and the AI value of the bare land type increased from 63.97 in 2017 to 78.25 in 2020, indicating that the connectivity between landscape patches in the study area was enhanced in the later stage of the earthquake. This also reflects that the ecological environment of the study area gradually showed a good trend in the later stage of the earthquake. The results of this paper not only reveal the spatial–temporal evolution of land cover in Jiuzhaigou County before and after the earthquake, but also help the relevant national decision-making departments to formulate corresponding policies and measures.
Food Sustainability Study in Ecuador: Using PCA Biplot and GGE Biplot
Authors: Valenzuela-Cobos Juan Diego , Guevara-Viejó Fabricio , Vicente-Galindo Purificación , Galindo-Villardón Purificación
Published: October 2022

Agriculture is one of the main sectors of Ecuador’s economy, and the principal agricultural product for exportation is cocoa. Flour samples of two mixtures were taken: a total of 50 samples of 85% cocoa bean shell (harvested from a farm) mixed with 15% soy flour (Mixture 1) and 50 samples of 75% cocoa bean shell (harvested from a farm) mixed with 25% soy flour (Mixture 2). The parameters evaluated were: moisture, protein, fat, carbohydrates, ash, total dietary fiber (TDF), and biological activity. Multivariate statistical techniques, such as PCA biplots and GGE biplots, were used to present each parameter (vector) measured. The biplot techniques suggested that the flour samples corresponding to Mixture 1 indicated the most significant values of nutritional and commercial properties. The results suggest that the use of mixtures of cocoa bean shell flour with soy flour can be used as ingredients to produce new foods.
Multidimensional Measurement and Enhancement Strategies of the Provincial Ecological Niche in the Yellow River Basin
Authors: Wu Yanxia , Yang Shuaishuai , Li Yushu
Published: October 2022

Provincial economic development in the VUCA era (V: volatility; U: uncertainty; C: complexity; A: ambiguity) is facing great challenges. Comprehensively measuring the provincial ecological niche and giving enhancement strategies are significant for the implementation of the Yellow River Strategy. By constructing the S–R–F (Status–Relationship–Function) provincial ecological niche measurement framework using the SEM model (the structural equation modeling), this paper calculated the provincial ecological niche of the Yellow River Basin from 2005 to 2019 using an ecological niche width model, an ecological niche overlap model and an ecological niche suitability model and gave improvement strategies through spatial positioning. Results: (1) The ecological niche breadth in the Yellow River Basin is uneven, showing a spatial pattern of “low in the upper reaches, high in the lower reaches”. The ecological niche overlap changes from synchronous competition to hierarchical differentiation. The ecological niche suitability shows a transformation of “overall homogeneity but local variation”. (2) Qinghai and Gansu belong to subspace I of ecological niches; the strategies of ecological niche specialization, potential ecological niche and ecological niche synergy are appropriate. Shanxi, Inner Mongolia and Ningxia belong to subspace III; the strategies of ecological niche specialization, ecological niche separation, potential ecological niche and ecological niche synergy are suitable. Shandong belongs to subspace VI; the strategies of ecological niche generalization and ecological niche synergy are fitting. Henan, Sichuan and Shaanxi belong to subspace VIII; it is advisable to choose ecological niche generalization, ecological niche separation and ecological niche synergy strategies. The possible marginal contributions of this paper are: (1) applying the idea of fitting optimization to guide the construction of a provincial ecological status indicator system, applying the validation analysis in the SEM model to test the suitability and validity of a provincial ecological status indicator system and improving the scientificity and objectivity of the indicator system construction. (2) Correlating the measurement model with the connotation (state–relationship–function) of the provincial ecological niche, the perspective of ecological niche research and improving the theoretical support for the construction of a multidimensional measurement model. (3) Spatial positioning of provincial ecological niches to improve the relevance and effectiveness of strategies.
A Neutral Heteroleptic Molybdenum Cluster trans-[{Mo6I8}(py)2I4]
Authors: Marchuk Margarita V. , Vorotnikov Yuri A. , Ivanov Anton A. , Eltsov Ilia V. , Kuratieva Natalia V. , Shestopalov Michael A.
Published: October 2022

Despite that the chemistry of octahedral cluster complexes has been actively developed recently, there are still a lot of unexplored areas. For example, to date, only a few halide M6-clusters with N-heterocycles are known. Here, we obtained an apically heteroleptic octahedral iodide molybdenum cluster complex with pyridine ligands—trans-[{Mo6I8}(py)2I4] by the direct substitution of iodide apical ligands of [{Mo6I8}I6]2– in a pyridine solution. The compound co-crystalized with a monosubstituted form [{Mo6I8}(py)I5]– in the ratio of 1:4, and thus, can be described by the formula (pyH)0.2[{Mo6I8}(py)1.8I4.2]·1.8py. The composition was studied using XRPD, elemental analyses, and 1H-NMR and IR spectroscopies. According to the absorption and luminescence data, the partial substitution of apical ligands weakly affects optical properties.
The Migration Pattern of Atrazine during the Processes of Water Freezing and Thawing
Authors: Zhang Yan , Zhao Chen , Yu Aixin , Zhao Wanli , Ren Fangyun , Liu Yucan
Published: October 2022

Atrazine, one of the most commonly used herbicides in the world, is of concern because of its frequent occurrence in various water bodies and the potential threat it constitutes to ecosystems. The transport of contaminants in seasonally ice-covered lakes is an important factor affecting the under-ice water environment, and changes in phase during ice growth and melting cause redistribution of atrazine between ice and water phases. To explore the migration pattern of atrazine during freezing and thawing, laboratory simulation experiments involving freezing and thawing were carried out. The effects of ice thickness, freezing temperature, and initial concentration on the migration ability of atrazine during freezing were investigated. The results showed that the relationship between the concentration of atrazine in ice and water during freezing was ice layer < water before freezing < water layer under the ice. Atrazine tended to migrate to under-ice water during the freezing process, and the intensity of migration was positively correlated with the ice thickness, freezing temperature, and initial concentration. During the thawing phase, atrazine trapped in the ice was released into the water in large quantities in the early stages. The first 20% of meltwater concentration was significantly higher than the average concentration in ice, with the highest case being 2.75 times the average concentration in ice. The results reported in this study are a useful reference for planning possible pollution control measures on such lakes during their freeze-thaw process.
Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin
Authors: Cardoso Pedro Henrique de Oliveira , Boleti Ana Paula de Araújo , Silva Patrícia Souza e , Mukoyama Lincoln Takashi Hota , Guindo Alexya Sandim , Moraes Luiz Filipe Ramalho Nunes de , Oliveira Caio Fernando Ramalho de , Macedo Maria Ligia Rodrigues , Carvalho Cristiano Marcelo Espínola , de Castro Alinne Pereira , Migliolo Ludovico
Published: October 2022

The importance of neuroinflammation in neurology is becoming increasingly apparent. In addition to neuroinflammatory diseases such as multiple sclerosis, the role of neuroinflammation has been identified in many non-inflammatory neurological disorders such as stroke, epilepsy, and cancer. The immune response within the brain involves the presence of CNS resident cells; mainly glial cells, such as microglia, the CNS resident macrophages. We evaluated the peptide Ca-MAP1 bioinspired on the C. albicans immature cytolytic toxin candidalysin to develop a less hemolytic peptide with anti-neuroinflammatory, antibacterial, and cytotoxic activity against tumor cells. In silico and in vitro studies were performed at various concentrations. Ca-MAP1 exhibits low hemolytic activity at lower concentrations and was not cytotoxic to MRC-5 and BV-2 cells. Ca-MAP1 showed activity against Acinetobacter baumannii, Escherichia coli ATCC, E. coli KPC, Klebsiella pneumoniae ATCC, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC. Furthermore, Ca-MAP1 exhibits anti-neuroinflammatory activity in the BV-2 microglia model, with 93.78% inhibition of nitrate production at 18.1 µM. Ca-MAP1 presents cytotoxic activity against tumor cell line NCI-H292 at 36.3 μM, with an IC50 of 38.4 µM. Ca-MAP1 demonstrates results that qualify it to be evaluated in the next steps to promote the control of infections and provide an alternative antitumor therapy.
The Validation of FORMOSAT-3/COSMIC Measurements in the Middle Latitude Region of China with Ionosonde Observations during 2015–2018
Authors: Hu Liangchen , Su Fanfan , Zhu Fuying , Li Xinxing
Published: October 2022

We used ground-based ionosonde observations at Ganzi (31.2° N, 100.4° E) to validate the COSMIC measurement in the middle latitude region of China during low solar activity. First, eligible data pairs from two kinds of techniques were selected for the validation. Then, we investigated the consistency of the ionospheric parameters’ F layer peak density (NmF2) from selected data pairs at different local times in different seasons, and we also investigated the F layer peak height (hmF2). The correlation of the parameters (including NmF2 and hmF2) were good in general. The correlation coefficients of the NmF2 and hmF2 from all selected data pairs were 0.94 and 0.77, respectively. The correlation coefficients were higher in the daytime than those at night for both the NmF2 and hmF2. The correlation coefficients in different seasons were close to each other for both the NmF2 and hmF2. The NmF2 from the COSMIC tends to be overestimated during the whole day except in the morning; the hmF2 from the COSMIC tends to be overestimated in the morning and underestimated in the afternoon.
Clinicopathological and Epidemiological Findings in Pet Cats Naturally Infected with Feline Immunodeficiency Virus (FIV) in Australia
Authors: Carlton Caroline , Norris Jacqueline M. , Hall Evelyn , Ward Michael P. , Blank Stephanie , Gilmore Shelby , Dabydeen Anjuli , Tran Vivian , Westman Mark E.
Published: October 2022

Feline immunodeficiency virus (FIV) infection in experimentally infected domestic cats produces characteristic clinical manifestations including hematological changes, neurological disease, neoplasia (most notably lymphoma) and lymphopenia-mediated immunodeficiency predisposing cats to a range of secondary infections. Conflicting reports exist, however, with regard to disease associations and survival time in naturally FIV-infected cats. The purpose of this retrospective case–control study was to investigate the effect of natural FIV infection on hematological, blood biochemical and urinalysis parameters and survival time in three cohorts of pet cats in Australia. Cohorts 1 and 2 were recruited from a large veterinary hospital in Melbourne, Victoria (n = 525 and 282), while a third cohort consisted of cats recruited from around Australia as part of a FIV field vaccine efficacy trial (n = 425). FIV-infected cats in cohorts 1, 2 and 3 were found to have 15/37 (41%), 13/39 (33%) and 2/13 (15%) clinicopathological parameters significantly different to FIV-uninfected cats, respectively. Two changes in FIV-infected cats in cohort 1, hypochromia (low hemoglobin) and hyperglobulinemia, were outside the supplied reference intervals and should serve as diagnostic triggers for FIV testing. Kaplan–Meier survival analysis of cats in cohorts 1 and 2 combined did not find any difference between FIV-infected and FIV-uninfected cats, however a confounding factor was a large euthanasia rate within the first 12 months in both groups. Three significant (p < 0.05) spatial clusters of FIV infection were identified in Melbourne. A possible relationship between FIV infection status and socioeconomic disadvantage was discovered, based on three government indices of socioeconomic status (p < 0.001). Until longitudinal field studies are performed in Australia to further investigate the long-term effects of natural FIV infection, Australian veterinarians should consider FIV to be an important infection of pet cats, and recommend measures to prevent FIV infection.
HIV-1 Tat Upregulates the Receptor for Advanced Glycation End Products and Superoxide Dismutase-2 in the Heart of Transgenic Mice
Authors: Qrareya Alaa N. , Wise Nason S. , Hodges Emmanuel R. , Mahdi Fakhri , Stewart James A. , Paris Jason J.
Published: October 2022

Cardiovascular disorder (CVD) is a common comorbidity in people living with HIV (PLWH). Although the underlying mechanisms are unknown, virotoxic HIV proteins, such as the trans-activator of transcription (Tat), likely contribute to CVD pathogenesis. Tat expression in mouse myocardium has been found to induce cardiac dysfunction and increase markers of endothelial toxicity. However, the role that Tat may play in the development of CVD pathogenesis is unclear. The capacity for Tat to impact cardiac function was assessed using AC16 human cardiomyocyte cells and adult male and female transgenic mice that conditionally expressed Tat [Tat(+)], or did not [Tat(−)]. In AC16 cardiomyocytes, Tat increased intracellular calcium. In Tat(+) mice, Tat expression was detected in both atrial and ventricular heart tissue. Tat(+) mice demonstrated an increased expression of the receptor for advanced glycation end products and superoxide dismutase-2 (SOD-2) in ventricular tissues compared to Tat(−) controls. No changes in SOD-1 or α-smooth muscle actin were observed. Despite Tat-mediated changes at the cellular level, no changes in echocardiographic measures were detected. Tat(+) mice had a greater proportion of ventricular mast cells and collagen; however, doxycycline exposure offset the latter effect. These data suggest that Tat exposure promotes cellular changes that can precede progression to CVD.
SARS-CoV-2 Transmission in Belgian French-Speaking Primary Schools: An Epidemiological Pilot Study
Authors: Frère Julie , Chatzis Olga , Cremer Kelly , Merckx Joanna , De Keukeleire Mathilde , Renard Florence , Ribesse Nathalie , Minner Frédéric , Ruelle Jean , Kabamba Benoit , Rodriguez-Villalobos Hector , Bearzatto Bertrand , Delforge Marie-Luce , Henin Coralie , Bureau Fabrice , Gillet Laurent , Robert Annie , Van der Linden Dimitri
Published: October 2022

Schools have been a point of attention during the pandemic, and their closure one of the mitigating measures taken. A better understanding of the dynamics of the transmission of SARS-CoV-2 in elementary education is essential to advise decisionmakers. We conducted an uncontrolled non-interventional prospective study in Belgian French-speaking schools to describe the role of attending asymptomatic children and school staff in the spread of COVID-19 and to estimate the transmission to others. Each participant from selected schools was tested for SARS-CoV-2 using a polymerase chain reaction (PCR) analysis on saliva sample, on a weekly basis, during six consecutive visits. In accordance with recommendations in force at the time, symptomatic individuals were excluded from school, but per the study protocol, being that participants were blinded to PCR results, asymptomatic participants were maintained at school. Among 11 selected schools, 932 pupils and 242 school staff were included between January and May 2021. Overall, 6449 saliva samples were collected, of which 44 came back positive. Most positive samples came from isolated cases. We observed that asymptomatic positive children remaining at school did not lead to increasing numbers of cases or clusters. However, we conducted our study during a period of low prevalence in Belgium. It would be interesting to conduct the same analysis during a high prevalence period.
Imbalanced Angiogenesis in Pregnancies Complicated by SARS-CoV-2 Infection
Authors: Giardini Valentina , Ornaghi Sara , Gambacorti-Passerini Carlo , Casati Marco , Carrer Andrea , Acampora Eleonora , Vasarri Maria Viola , Arienti Francesca , Vergani Patrizia
Published: October 2022

COVID-19 and preeclampsia (preE) share the ANG-II mediated endothelial dysfunction, resulting from a significant dysregulation of RAS and an imbalanced proportion of anti-angiogenic and pro-angiogenic soluble plasmatic factors. Of note, an increased incidence of preE has been reported among COVID-19-infected mothers compared to the general pregnant population. The two most promising angiogenic markers are the soluble fms-like tyrosine kinase receptor-1 (sFlt-1), the major antiangiogenic factor, and the placental growth factor (PlGF), a powerful angiogenic factor. Since these markers have proven useful in the prediction, diagnosis, and severity of preE, this study aimed to evaluate their maternal serum levels in pregnancies complicated by SARS-CoV-2 infection and to assess their potential use to guide the management of these women. A retrospective analysis of SARS-CoV-2-positive pregnant women was performed. The serum levels of sFlt-1 and PlGF were collected at the diagnosis of SARS-CoV-2 infection at the hospital, before the beginning of steroid/hydroxychloroquine and/or antithrombotic therapy. The sFlt-1/PlGF ratio was stratified using cut-off values clinically utilized in the diagnosis and prediction of preE (low < 38, intermediate 38–85/110* and high >85/110*, * if before or after the 34th week of gestation). A total of 57 women were included, of whom 20 (35%) had signs and symptoms of COVID-19 at hospital presentation and 37 (65%) were asymptomatic. None were vaccinated. The mean gestational age at diagnosis of SARS-CoV-2 infection was 32 weeks in symptomatic patients and 37 weeks and 5 days in asymptomatic ones (p = 0.089). sFlt-1 serum levels were higher in SARS-CoV-2 positive asymptomatic patients compared to women with COVID-19 related symptoms (4899 ± 4357 pg/mL vs. 3187 ± 2426 pg/mL, p = 0.005). sFlt-1/PlGF at admission was <38 in 18 of the 20 symptomatic women (90%) compared to 22 (59%) of the asymptomatic patients (p = 0.018). Of note, two of the three women admitted to the intensive care unit had a very low ratio (<2). In turn, rates of patients with sFlt-1/PlGF at admission > 85/110 were not significantly different between the two groups: 11% in asymptomatic patients (4/37) vs. none of the symptomatic patients (p = 0.286), and all of them presented a placental dysfunction, like preE (n = 1) and FGR (n = 3). Of note, there were no stillbirths or maternal or neonatal deaths among symptomatic patients; also, no cases of preE, FGR, or small for gestational age neonates were diagnosed. In conclusion, our data suggest that SARS-CoV-2 infection during pregnancy could influence the angiogenic balance. A significant pathological alteration of the sFlt-1/PlGF ratio cannot be identified during the symptomatic phase; however, if left untreated, SARS-CoV-2 infection could potentially trigger placental dysfunction.
Transfusion-Acquired HIV: History, Evolution of Screening Tests, and Current Challenges of Unreported Antiretroviral Drug Use in Brazil
Authors: Nishiya Anna S. , Ferreira Suzete C. , Salles Nanci A. , Rocha Vanderson , Mendrone-Júnior Alfredo
Published: October 2022

Prevention of HIV acquisition by blood transfusion from its emergence to the present day is reviewed, and current challenges are delineated. The experience of Fundação Pró-Sangue/Hemocentro de São Paulo, Brazil, is highlighted in the quest for improvements in blood safety and the evolution of increasingly sensitive and specific screening tests. Concerns and establishing stringent criteria in the screening of potential blood donors are emphasized, and the current criteria for identifying and deferring candidates at high risk of acquiring sexually transmitted diseases are summarized. Future challenges relate to the identification of donors with unreported use of antiretroviral drugs for prophylaxis against possible HIV exposure or for treatment of an HIV infection whose viral expression is undetectable by current analyses. There is a need to better understand the motivation of HIV-exposed donors and to educate them about the risk of transfusion-mediated HIV transmission despite having low or undetectable viral loads. In situations in which traditional HIV RNA or antibody detection assays remain negative, more sensitive analyses are needed to identify potential donors at risk for HIV transmission.
Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait
Authors: Lakshmanan Renuk , Mietzsch Mario , Jimenez Ybargollin Alberto , Chipman Paul , Fu Xiaofeng , Qiu Jianming , Söderlund-Venermo Maria , McKenna Robert
Published: October 2022

Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans, are parvoviruses belonging to the genera Amdoparvovirus and Tetraparvovirus, respectively. While Aleutian mink disease caused by AMDV is a major threat to mink farming, no clear clinical manifestations have been established following infection with PARV4 in humans. Here, the capsid structures of AMDV and PARV4 were determined via cryo-electron microscopy at 2.37 and 3.12 Å resolutions, respectively. Despite low amino acid sequence identities (10–30%) both viruses share the icosahedral nature of parvovirus capsids, with 60 viral proteins (VPs) assembling the capsid via two-, three-, and five-fold symmetry VP-related interactions, but display major structural variabilities in the surface loops when the capsid structures are superposed onto other parvoviruses. The capsid structures of AMDV and PARV4 will add to current knowledge of the structural platform for parvoviruses and permit future functional annotation of these viruses, which will help in understanding their infection mechanisms at a molecular level for the development of diagnostics and therapeutics.
Neotropical Sylvatic Mosquitoes and Aedes aegypti Are Not Competent to Transmit 17DD Attenuated Yellow Fever Virus from Vaccinated Viremic New World Non-Human Primates
Authors: de Miranda Rafaella Moraes , Fernandes Rosilainy Surubi , da Silva-Fernandes André Tavares , Ferreira-de-Brito Anielly , Moreira Silvia Bahadian , Pereira Renata Carvalho , da Silva Mendes Ygara , de Lima Sheila Maria Barbosa , Pissinatti Alcides , Freire Marcos da Silva , Alencar Jerônimo Augusto Fonseca , Lourenco-de-Oliveira Ricardo
Published: October 2022

Beside humans, thousands of non-human primates (NHPs) died during the recent outbreak caused by the yellow fever virus (YFV) in Brazil. Vaccination of NHPs against YFV with the YF 17DD attenuated virus has emerged as a public health strategy, as it would reduce sylvatic transmission while also preserving endangered susceptible species. The hypothesis of establishing an uncontrolled transmission of this attenuated virus in nature was raised. We assessed vector competence of four sylvatic mosquito species, Haemagogus leucocelaenus, Haemagogus janthinomys/capricornii, Sabethes albiprivus, and Sabethes identicus, as well as the urban vector Aedes aegypti for YF 17DD attenuated vaccine virus when fed directly on eleven viremic lion tamarins or artificially challenged with the same virus. No infection was detected in 689 mosquitoes engorged on viremic lion tamarins whose viremia ranged from 1.05 × 103 to 6.61 × 103 FFU/mL, nor in those artificially taking ≤ 1 × 103 PFU/mL. Low viremia presented by YF 17DD-vaccinated New World NHPs combined with the low capacity and null dissemination ability in sylvatic and domestic mosquitoes of this attenuated virus suggest no risk of its transmission in nature. Thus, vaccination of captive and free-living NHPs against YFV is a safe public health strategy.
The Association between Socio-Demographics and Mental Distress Following COVID-19 Vaccination—Mediation of Vaccine Hesitancy
Authors: Zhang Xiaoying , Shen Junwei , Li Ming , Shi Yijian , Wang Qing , Chen Fazhan , Qin Hongyun , Zhao Xudong
Published: October 2022

The COVID-19 vaccine has been administered to over 200 countries and regions. With the unprecedented vaccination scale and speed, vaccination correlated mental health issues should be paid precise attention to. This study aims to assess the association between socio-demographic factors and mental health following vaccination and to analyze the mediation effect of vaccine hesitancy. This study recruited 2112 individuals who took two doses of the COVID-19 vaccine in Shanghai. Structural equation modeling was performed to assess factors associated with anxiety and depression of the vaccinated individuals and the underlying mechanism. The results yielded that vaccine hesitancy partially mediated/suppressed the effect from gender and employment status to anxiety/depression and fully mediated the effects from education to anxiety/depression. This study advanced the understanding of mental health disparity among different socio-demographic groups after vaccination and the impact of vaccine hesitancy on the vaccinated population’s mental health. The finding offered insights into the possible mental vulnerability of people holding a hesitant attitude before vaccination and suggested that vaccine hesitancy played a crucial role in people’s mental health after vaccination. Health promotion programs can target vaccine hesitancy to prevent unfavorable mental health consequences among specific populations.
Global Excess Mortality during COVID-19 Pandemic: A Systematic Review and Meta-Analysis
Authors: Shang Weijing , Wang Yaping , Yuan Jie , Guo Zirui , Liu Jue , Liu Min
Published: October 2022

Background: Currently, reported COVID-19 deaths are inadequate to assess the impact of the pandemic on global excess mortality. All-cause excess mortality is a WHO-recommended index for assessing the death burden of COVID-19. However, the global excess mortality assessed by this index remains unclear. We aimed to assess the global excess mortality during the COVID-19 pandemic. Methods: We searched PubMed, EMBASE, and Web of Science for studies published in English between 1 January 2020, and 21 May 2022. Cross-sectional and cohort studies that reported data about excess mortality during the pandemic were included. Two researchers independently searched the published studies, extracted data, and assessed quality. The Mantel–Haenszel random-effects method was adopted to estimate pooled risk difference (RD) and their 95% confidence intervals (CIs). Results: A total of 79 countries from twenty studies were included. During the COVID-19 pandemic, of 2,228,109,318 individuals, 17,974,051 all-cause deaths were reported, and 15,498,145 deaths were expected. The pooled global excess mortality was 104.84 (95% CI 85.56–124.13) per 100,000. South America had the highest pooled excess mortality [134.02 (95% CI: 68.24–199.80) per 100,000], while Oceania had the lowest [−32.15 (95% CI: −60.53–−3.77) per 100,000]. Developing countries had higher excess mortality [135.80 (95% CI: 107.83–163.76) per 100,000] than developed countries [68.08 (95% CI: 42.61–93.55) per 100,000]. Lower middle-income countries [133.45 (95% CI: 75.10–191.81) per 100,000] and upper-middle-income countries [149.88 (110.35–189.38) per 100,000] had higher excess mortality than high-income countries [75.54 (95% CI: 53.44–97.64) per 100,000]. Males had higher excess mortality [130.10 (95% CI: 94.15–166.05) per 100,000] than females [102.16 (95% CI: 85.76–118.56) per 100,000]. The population aged ≥ 60 years had the highest excess mortality [781.74 (95% CI: 626.24–937.24) per 100,000]. Conclusions: The pooled global excess mortality was 104.84 deaths per 100,000, and the number of reported all-cause deaths was higher than expected deaths during the global COVID-19 pandemic. In South America, developing and middle-income countries, male populations, and individuals aged ≥ 60 years had a heavier excess mortality burden.
Cranial Spinal Spreading of Canine Brain Gliomas after Hypofractionated Volumetric-Modulated Arc Radiotherapy and Concomitant Temozolomide Chemotherapy: A Four-Case Report
Authors: Urso Gaetano , Boncu Alexandra , Carrara Nancy , Zaman Dragos-Teodor , Malfassi Luca , Marcarini Silvia , Minoli Lucia , Pavesi Simone , Sala Massimo , Scanziani Eugenio , Dolera Mario
Published: October 2022

Gliomas are the second-most-common primary brain tumors in dogs. Surgery and radiotherapy are established treatment approaches with similar median survival time, whereas conventional chemotherapy is burdened by severe adverse effects. Spinal and leptomeningeal spread of gliomas have been described following radiotherapy treatment alone. The purpose of this study was to evaluate the outcome for four dogs with primary high-grade gliomas in the forebrain without evidence, at diagnosis, of neoplastic invasion along the spinal cord, that were treated with concomitant chemotherapy (temozolomide) and hypofractionated volumetric-modulated arc radiotherapy (VMAT-RT). Temozolomide was selected for its radiosensitive properties, and radiotherapy dose protocols of 37 Gy in 7 fractions or 42 Gy in 10 fractions were used. After an initial complete or partial response, tumors recurred across the cranial–spinal pathway. Post-mortem macroscopic examinations confirmed swollen spinal cord and hyperemic meningeal sleeve, with nodular lesions on the meningeal surface. Microscopically, infiltration of the spinal cord and meninges by neoplastic cells (with features of oligodendrogliomas) were observed. This work seems to suggest that the entire central nervous system should be investigated in diagnostic examinations of canine gliomas. Dose-escalation trials and/or spinal cord prophylaxis treatment could also be evaluated to prevent tumor progression.
Are Horse Owners Able to Estimate Their Animals’ Body Condition Score and Cresty Neck Score?
Authors: Busechian Sara , Turini Luca , Sgorbini Micaela , Pieramati Camillo , Pisello Lorenzo , Orvieto Simona , Rueca Fabrizio
Published: October 2022

Obesity and overconditioning are becoming more prevalent in horses, and are influencing the animal’s health, activity, and reproductive efficiency. Body condition score (BCS) and cresty neck score (CNS) have been correlated with the development of various diseases. Many of the papers in the literature evaluate the prevalence of obesity and overconditioning in horse populations considering BCS given by owners. The aim of this study was to evaluate the validity of the owners’ estimation of the degree of equine obesity or overconditioning by comparing BCS and CNS provided by each owner and a board-certified vet to a cohort of 259 horses and their agreement based on age, breed, sex, and activity of the animals. Overconditioning was present in 29% and 24% of horses according to the owners and the vet, respectively, and obesity in 2% and 1%, respectively. Agreement between BCS and CNS given by the owners and the vet was slight, with owners scoring horses either lower or higher than the vet. Agreement between the owners and the vet assessment was also influenced by age, breed, sex, and activity of the animals. Education of owners and caretakers to correctly assess BCS and CNS and regular evaluation of the animals throughout the year is important to prevent the development of obesity and overconditioning and related diseases.
Skin Photodamage Lesions in a Bilateral Feline Auricular Primary Fibrosarcoma
Authors: Parisi Francesca , Abramo Francesca , Maimone Marco , Poli Alessandro , Millanta Francesca
Published: October 2022

As with human species, recent studies also suggest a photoinduced etiopathology for non-epithelial cutaneous tumors in feline species. We report a recent case of a ten-year-old male cat with a white-hair coat and mesenchymal neoplasms of both auricles. Cytology, complete blood count (CBC), serum biochemistry and imaging examinations were performed. After surgery, the samples underwent routinary histopathology and were additionally stained with orcein. A routine analysis yielded values within a normal range and the imaging examination showed no abnormalities, suggesting that the bilateral presentation of neoplasms was primary rather than metastatic. The cytology was inconclusive, but, through histopathology, two well-differentiated fibrosarcomas were diagnosed and histopathological changes related to chronic UV exposure (such as epidermal hyperplasia, stratification disorders, keratinocyte dysplasia and an accumulation of elastotic material) were documented in the skin adjacent to the lesions. An orcein stain succeeded in highlighting elastosis. The elastic fibers lost their regular structure and orientation and appeared to be fragmented, wavy to branched and knotted. A morphometric analysis showed that the amount of elastotic material in the dermis close to the tumors was more than double compared with the more distant areas. Elastosis is considered to be a hallmark of photodamage; thus, an involvement of UV rays in the carcinogenic process of the tumors may be suspected.
Effects of Feeding Housefly (Musca domestica) Larvae on the Butchery Skills and Meat Sensory Characteristics of Local Chickens in Niger
Authors: Hamani Bachir , Taffa Adamou Guisso , Issa Salissou , Mahamadou Chaibou , Detilleux Johann , Moula Nassim
Published: October 2022

The purpose of this work was to study the effects of substitution of fishmeal by housefly larvae at different rates and different physical states in the diet of local chickens. Five diets consisted of LFD, 25DL, 50DL, 25FL and 50FL, respectively, larval-free, 25%-dried-larvae, 50%-dried-larvae, 25%-fresh-larvae and 50%-fresh-larvae diet, in which 0, 25 and 50% of fishmeal was replaced by dried and fresh larvae, was formulated. A total of 165 local chickens of three weeks old, divided into 15 boxes in batches of 11 animals were raised. The experiment consists of three replications of five treatments. At 14 weeks of age, sixty chickens were slaughtered. Butchery skills and sensory characteristics were evaluated. Thus, a small variation of the ultimate pH from 5.63 to 5.55 between the different types of meat, and a carcass yield around 66%, was recorded. Any effects of substitution rate and physical state of housefly larvae on growth performance was not observed. Feet and proventriculus percentages increased in chickens fed 25% substitution. Liver and spleen percentage, and redder breast meat, increased in chicken fed dried larvae. Yellowness of the breast, thigh-and-drumstick meat and juiciness increased with 50% substitution. There is need of an investigation for liver and spleen enlargement and housefly larvae containing pigments. Thus, housefly larvae up to 50% substitution can be a suitable alternative to fishmeal in local chicken diets.
Risk Factors Regarding Dog Euthanasia and Causes of Death at a Veterinary Teaching Hospital in Italy: Preliminary Results
Authors: Pugliese Michela , Falcone Annastella , Alibrandi Angela , Zirilli Agata , Passantino Annamaria
Published: October 2022

The decision to request and proceed with euthanasia in a dog is complex and predictors of such decisions are important. This study investigates the risk factors (demographic and clinical) associated with the main clinical causes and methods of death (euthanasia or unassisted death) in a population of dogs. By comparing euthanasia to unassisted deaths, the authors assess causes of death to evaluate their relative impacts on decision-making to choose euthanasia compared with an unassisted death. For this, goal data from electronic medical records of dogs who had died (unassisted death and euthanasia), obtained from an Italian referral veterinary teaching hospital from 2010 to 2020, were analyzed. The causes of death were categorized by pathophysiological process and the organ system. Univariate and multivariable logistic regression analyses were performed to identify the factors that significantly affect the probability of undergoing euthanasia and to individuate independent significant predictors of euthanasia and unassisted death, respectively. Death rate by euthanasia was 40.7% (125/307), by died unassisted 50.8% (156/307), whilst only 8.5% of dogs (26/307) died accidentally. The main causes of death for euthanasia were due to neoplastic (75.6%), degenerative (64.3%), and congenital (60%) diseases. Furthermore, the findings reveal that in deaths by euthanasia, the significant risk predictors were female gender, age, and neoplastic and degenerative processes; while in unassisted deaths, the significant risk predictors were male gender, age, and infection/inflammatory conditions. These preliminary outcomes highlight the information of this study which may be used to evaluate strategic interventions and health promotion strategies to be implemented, with consequent welfare gains for the canine population.
Effectiveness of Intramammary Antibiotics, Internal Teat Sealants, or Both at Dry-Off in Dairy Cows: Milk Production and Somatic Cell Count Outcomes
Authors: ElAshmawy Wagdy R. , Okello Emmanuel , Williams Deniece R. , Anderson Randall J. , Karle Betsy , Lehenbauer Terry W. , Aly Sharif S.
Published: October 2022

Mastitis is the greatest disease challenge for dairy producers, with substantial economic impacts due to lost milk production. Amongst the approaches implemented to control and prevent mastitis on dairies are vaccination, pre- and post-milking teat dips, and treatments at dry-off including intramammary antibiotics and teat sealants. The objectives of our study were to evaluate the effect of different treatments at dry-off on the subsequent lactation’s milk production and somatic cell count (SCC). A single-blinded controlled block randomized clinical trial was conducted between December 2016 and August 2018 on eight herds from four of the top ten milk-producing counties in California: Tulare, Kings, Stanislaus, and San Joaquin. The trial was repeated with cows enrolled during the winter and summer seasons to account for seasonal variability. Eligible cows were treated at dry-off with either intramammary antibiotics (AB), internal teat sealant (TS), AB + TS, or did not receive any treatment (control), and were followed through 150 days in milk (DIM) post-calving. The milk production and SCC data were extracted from monthly test day milk records (Dairy Comp 305, Valley Ag Software, Tulare, CA, USA). Two-piece spline linear mixed models were used to model the milk production (kg) and natural logarithm-transformed SCC. After accounting for parity, breed, season, and dry period duration, the milk model showed a significant increase in milk production (1.84 kg/day) in cows treated with AB + TS at dry-off in comparison to controls. There was no significant difference in the milk produced by cows that received either AB or TS (0.12 kg/day, and 0.67 kg/day, respectively) in comparison to the untreated cows. Different dry cow treatments were associated with a significant reduction in ln SCC during the first 150 DIM. The greatest reduction was associated with using AB + TS, followed by AB, and finally TS in comparison to controls. Dairies with high SCC may benefit from treating cows at dry-off with AB, TS, or both.
From Sponge Cities to Sponge Watersheds: Enhancing Flood Resilience in the Sishui River Basin in Zhengzhou, China
Authors: Peng Xiao , Heng Xianpei , Li Qing , Li Jianxia , Yu Kongjian
Published: October 2022

Increasing extreme rainfall and increasingly severe flood disasters worldwide make it essential to enhance flood resilience research and practice. The Sponge City Approach (SCA) has been proposed as a nature-based solution for solving pressing water problems in cities, but there is an urgent need to apply the approach at the broader catchment scale to address the rising challenges driven by climate change. This study established a Sponge Watershed Approach (SWA) using the “Source-Flow-Sink” framework and applied it in the Sishui River Basin in Zhengzhou, China, to reconstruct a town after a heavy rainfall disaster and enhance its resilience to future floods. An integrated modeling approach utilizing hydrologic, hydraulic, and flood-loss curve models was used to analyze and evaluate the flood-reduction effects of different strategies under multiple scenarios. The results show that: (1) a strategy focusing solely on interventions at the source of runoff cannot adequately cope with extreme rainfall, and a whole-system approach to managing floods is necessary instead; (2) the effectiveness of the flow strategy depends on the intervention magnitude and the location where interventions are made; (3) the sink strategy offers the most effective way (approximately 30% decrease in flood loss) to adapt to extreme rainfall; (4) the risk and impact of flood disaster in the town was significantly reduced with the SWA (approximately 35–50% decrease in flood loss) compared to those without any interventions; (5) the effects of these strategies are interactive, and the optimal strategy depends on the rainfall condition. These results demonstrate the practicability of SWA by integrating the source, flow, and sink strategy at the whole-catchment scale, which will help promote natural flood management and enhance the climate resilience of catchments worldwide.
New Data on Reproductive System and Spermatozoa Confirm Macrodasys as a Model in Comparative Reproductive Analysis in Macrodasyida (Gastrotricha)
Authors: Guidi Loretta , Balsamo Maria , Grassi Eleonora , Semprucci Federica , Todaro M. Antonio
Published: October 2022

Gastrotricha Macrodasyida, aquatic worms, are primarily hermaphrodites with internal cross-fertilization and indirect sperm transfer. Insightful information on this matter dates to 1978 and derives from an illuminating work focused on two Macrodasys species. The role as a “model” of this taxon clashes, however, with the relative paucity of data concerning the ultrastructure of the accessory organs involved in the sperm transfer. Therefore, we have undertaken a new study on three additional species of Macrodasys focusing on the frontal and caudal organs and also on the spermatozoa. Our observations confirmed the structural plan of the reproductive system reported for the genus, but found some differences concerning the accessory reproductive organs. Most importantly, we found spermatozoa inside the caudal organ of several single specimens, suggestive of a more plausible hypothesis about sperm transfer modality in Macrodasys. In the spermatozoa, the position of the mitochondrion outside rather than inside the nucleus has been confirmed as a major difference between the spermatozoa of Macrodasys species and those of the other Macrodasyida. We suggest that the peculiar structure of the mature spermatozoon of Macrodasys could derive from an incomplete process of spermatogenesis and hence it would be useful to infer phylogenetic alliances.