bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of First Childbirth Characteristics between Elite Judo Athletes and Non-Athletes: The Preliminary Retrospective Case–Control Study
Authors: Kuczera Anna , Opala-Berdzik Agnieszka , Malá Jitka , Sodowski Marcin , Chmielewska Daria
Published: October 2022

It has been speculated that elite athletes are more likely to have obstetric interventions during labor and delivery. So far, the impact of many years of competitive sports participation on childbirth characteristics has not been well-established. This preliminary retrospective case–control study aimed to determine whether the first labors of elite judo competitors required obstetric interventions more frequently and were longer than those of non-athletes. The study comprised 32 parous women: 16 elite judo athletes and 16 non-athletes. Women were included if they had access to the following obstetric data (from their first childbirth hospital discharge reports and/or first child’s health record books): induction and augmentation of labor, mode of delivery, the duration of labor and its second stage, episiotomy, perineal tear, and the neonate’s Apgar score. Electronic surveys were completed concerning childbirth characteristics, level of “eliteness” (judo athletes), and recreational physical activity (non-athletes). The statistical analysis showed no significant between-group differences in any of the first childbirth outcomes under analysis. The preliminary results indicate elite judo practice before first pregnancy had no negative impact on the rates of labor induction and augmentation, delivery mode, duration of labor, the rates of episiotomy and perineal tear, and the neonate’s Apgar score. Due to the small sample size, the conclusions should be considered with caution.
Plastic Waste Degradation in Landfill Conditions: The Problem with Microplastics, and Their Direct and Indirect Environmental Effects
Authors: Wojnowska-Baryła Irena , Bernat Katarzyna , Zaborowska Magdalena
Published: October 2022

As landfilling is a common method for utilizing plastic waste at its end-of-life, it is important to present knowledge about the environmental and technical complications encountered during plastic disposal, and the formation and spread of microplastics (MPs) from landfills, to better understand the direct and indirect effects of MPs on pollution. Plastic waste around active and former landfills remains a source of MPs. The landfill output consists of leachate and gases created by combined biological, chemical, and physical processes. Thus, small particles and/or fibers, including MPs, are transported to the surroundings by air and by leachate. In this study, a special focus was given to the potential for the migration and release of toxic substances as the aging of plastic debris leads to the release of harmful volatile organic compounds via oxidative photodegradation. MPs are generally seen as the key vehicles and accumulators of non-biodegradable pollutants. Because of their small size, MPs are quickly transported over long distances throughout their surroundings. With large specific surface areas, they have the ability to absorb pollutants, and plastic monomers and additives can be leached out of MPs; thus, they can act as both vectors and carriers of pollutants in the environment.
A Meta-Analysis of the Effects of Different Training Modalities on the Inflammatory Response in Adolescents with Obesity
Authors: Zhao Haotian , Cheng Ruihong , Teng Jin , Song Ge , Huang Chenjian , Yuan Shuo , Lu Yuxuan , Shen Siqin , Liu Jingqi , Liu Chang
Published: October 2022

The aim of this study was to investigate the effect of different training modalities on improving the inflammatory response in adolescents with obesity. For the study methodology, the databases such as China National Knowledge Infrastructure (CNKI), Wanfang Data, Pubmed, Web of Science, and EBSCO were selected for searching. The methodological quality of the included studies was assessed using the Cochrane Risk of Bias (ROB) tool, and statistical analysis was performed by applying RevMan 5.4.1 analysis software. A total of 14 studies with 682 subjects were included. The results of this meta-analysis showed that aerobic training (AT) and aerobic plus resistance training (AT + RT) reduced the levels of IL-6 and CRP in adolescents with obesity. Among them, AT + RT was more effective than other training modalities in reducing IL-6 and CRP in adolescents with obesity. Different training modalities have no effect on the TNF-α level in adolescents with obesity. However, regarding the increase in IL-6, CRP, and TNF-α in adolescents with obesity, resistance training (RT) did not lead to significant differences. In conclusion, long-term regular AT, AT + RT, and HIIT are all helpful in improving the inflammatory state of adolescents with obesity, with AT + RT being the best training modality to combat inflammation in adolescents with obesity.
Re-Starting the Cruise Sector during the COVID-19 Pandemic in Greece: Assessing Effectiveness of Port Contingency Planning
Authors: Anagnostopoulos Lemonia , Kourentis Leonidas , Papadakis Antonios , Mouchtouri Varvara A.
Published: October 2022

Coronavirus disease (COVID-19) outbreaks on board cruise ships early in the pandemic highlighted gaps worldwide in public health emergency contingency plans (PHECPs) for responding to unknown threats. To restart cruise operations in 2021 and respond to potential COVID-19 outbreaks, a major tourist-based Greek island port (Port A) developed a COVID-19 PHECP. We assessed plan effectiveness by reviewing epidemiological data and monitoring outcomes, followed by an intra-action review (IAR) analyzing three event responses. From May to December 2021, 118 calls from 23 cruise ships with 119,930 passengers were recorded, with 29 COVID-19 cases in 11 cruises on board 7 ships. No outbreak was recorded during the study period. Strengths of the introduced PHECP included commitment of senior management; a core multi-disciplinary team of local authorities/ship agents involved in design and execution; interoperability agreements for port and ships’ PHECPs; cruise industry commitment to compliance; and pre-existing scenarios considering capacity needs. Central government coordination for preparedness planning at local ports is essential for successful responses. Monitoring local and country level response capacities is critical to inform planning, risk assessment, and decision-making. Immediately recording ports’ response actions provides the basis to capture lessons and improve contingency plans. To facilitate communication and common response protocols between European and non-European ports, IARs should be conducted between countries.
The Measurement of Love: Psychometric Properties and Preliminary Findings of the Short Love Scale (SLS-12) in a Polish Sample
Authors: Kozakiewicz Alicja , Izdebski Zbigniew , Mazur Joanna
Published: October 2022

There has been a perceived need for the development of instruments to assess relationship quality and love. The aim of this study was to describe the psychometric properties of the Short Love Scale (SLS-12) and to compare different measurement models. Data were collected using the CAWI (Computer-Assisted Web Interview) in Poland in early 2022 (18–60 years old; n = 941) among individuals living in formal or informal monogamous relationships. Both exploratory (EFA) and confirmatory factor (CFA) analyses were performed on two different subsamples obtained through random splitting of the full datafile. There were 12 items considered, which form three dimensions in accordance with the theoretical basis. CFA analysis confirmed good psychometric properties of the three-factor SLS-12 model based on EFA solution: χ2(47) = 146.802 (p ˂ 0.001); χ2/df = 3.123; CFI = 0.981, TLI = 0.973, RMSEA = 0.067 (90% CI 0.055–0.080), GFI = 0.952, AGFI = 0.921. SLS-12 subindices varied according to gender and relationship status. Based on ROC curve method, it may be assumed that scores on the SLS-12 ranging from 12 to 44 indicate a poor relationship, scores ranging from 45 to 52 a moderately good relationship, and scores of 53 to 60 a very good relationship. Improving and increasing the range of measures of love available to researchers remains an important task in supporting the progress of this area of research. Further research should be conducted among people of all ages living in both formal and informal relationships using the Short Love Scale-12 outlined in this paper.
The Relationship between Searches for COVID-19 Vaccines and Dynamics of Vaccinated People in Poland: An Infodemiological Study
Authors: Kłak Anna , Furmańczyk Konrad , Nowicka Paulina , Mańczak Małgorzata , Barańska Agnieszka , Religioni Urszula , Siekierska Anna , Ambroziak Martyna , Chłopek Magdalena
Published: October 2022

Background: Google Trends has turned out to be an appropriate tool for evaluating correlations and prognostic modelling regarding infectious diseases. The possibility of selecting a vaccine against COVID-19 has increased social interest in particular vaccines. The objective of this study was to show dependencies between the frequency of searches for COVID-19 vaccinations and the number of vaccinated people in Poland, along with epidemiological data. Methods: Data were collected regarding Google searches for COVID-19 vaccines, the number of people in Poland vaccinated against COVID-19, the number of new cases, and the number of deaths due to COVID-19. Data were filtered from 27 December 2020 to 1 September 2021. Results: The number of new vaccinations smoothed per million correlated most strongly with searches for the word ‘Pfizer’ in Google Trends (Kendall’s tau = 0.46, p < 0.001). The number of new deaths correlated most strongly with the search phrase ‘AstraZeneca’ (Kendall’s tau = 0.46, p < 0.001). The number of new cases per million correlated most strongly with searches for ‘AstraZeneca’ (Kendall’s tau = 0.49, p < 0.001). The maximum daily number of searches ranged between 110 and 130. A significant interest in COVID-19 vaccines was observed from February to June 2021, i.e., in the period of a considerable increase in the number of new cases and new deaths due to COVID-19. Conclusions: A significant increase in interest in COVID-19 vaccines was observed from February to June 2021, i.e., in the period of gradually extended access to vaccinations, as well as a considerable increase in the number of new cases and new deaths due to COVID-19. The use of Google Trends with relevant keywords and a comparison with the course of the COVID-19 pandemic facilitates evaluation of the relationship between the frequency and types of searches for COVID-19 vaccines and epidemiological data.
Opinions towards Medical Students’ Self-Care and Substance Use Dilemmas—A Future Concern despite a Positive Generational Effect?
Authors: McGurgan Paul , Calvert Katrina , Nathan Elizabeth , Celenza Antonio , Jorm Christine
Published: October 2022

This study examines demographic factors which may influence opinions concerning medical students’ self-care and substance use behaviors as a means of providing insights into how future doctors view these issues compared to Australian doctors and members of the public. We conducted national, multicenter, prospective, on-line cross-sectional surveys using hypothetical scenarios to three cohorts- Australian medical students, medical doctors, and the public. Participants’ responses were compared for the different contextual variables within the scenarios and the participants’ demographic characteristics. In total 2602 medical students, 809 doctors and 503 members of the public participated. Compared with doctors and the public, medical students were least tolerant of alcohol intoxication, and most tolerant of using stimulants to assist with study, and cannabis for anxiety. Doctor respondents more often aligned with the public’s opinions on the acceptability of the medical students’ behaviors. Although opinions are not equivalent to behaviour, Australian students’ views on the acceptability for cannabis to help manage anxiety, and inappropriate use of prescription-only drugs are concerning; these future doctors will be responsible for prescribing drugs and managing patients with substance abuse problems. However, if current Australian medical student’s opinions on alcohol misuse persist, one of the commonest substance addictions amongst doctors may decrease in future.
Species Diversity of Mycoplankton on the Background of Selected Indicators of Water Quality in Stratified Mesotrophic Lakes
Authors: Adam Cudowski , Magdalena Świsłocka
Published: October 2022

The aim of the study was to determine mycoplankton species diversity in relation to the physico-chemical parameters of lake waters. The research was carried out in the summer months in 15 mesotrophic lakes and showed a high ecological significance index for Rhodotorula glutinis, Epicoccum nigrum, Fusarium sporotrichioides, and Trichophyton violaceum. Mycoplankton abundance and species diversity decreased with the depth of water, which coincided with a decrease in oxygen content and organic matter concentration. A high concentration of nitrogen compounds (total nitrogen—TN and dissolved nitrogen—DN) limited the development of mycobiota in the hypolimnion. In the metalimnion, the intensive development of organisms, especially bacteria, limited mycoplankton abundance despite perfect physical and chemical conditions for its development. Finally, mycoplankton functioned the best in slightly alkaline waters.
Laser Treatment Increases the Antimicrobial Efficacy of Cyanobacterial Extracts against Staphylococcusaureus (SA) and Methicillin-resistantStaphylococcus aureus (MRSA)
Authors: Al Naim Haifa M. , El Semary Nermin
Published: October 2022

Staphylococcus aureus (SA) and Methicillin-resistant Staphylococcus aureus (MRSA) are multidrug-resistant bacterial pathogens. A novel approach needs to be followed to combat these pathogens in an ecofriendly manner. Cyanobacterial extracts were previously proven to be affective as antimicrobial agents. To capitalize on this, laser treatments were used to increase their antimicrobial efficacy. Two cyanobacterial strains isolated from Al-Ahsa were identified using molecular methods. Their aqueous extracts were used in the antimicrobial bioassay for these two bacterial pathogens. The first group of aqueous extracts were exposed directly to laser treatment and used in antibacterial bioassay. In parallel, the cyanobacterial biomass of the two isolates was exposed to the laser, then aqueous extracts were prepared. The third group of extracts were not exposed to the laser and were used as a control. Time and distance were the factors tested as they affected the dose of the laser, both individually and in combination. In addition, accessory pigment estimation in extracts before and after laser exposure of extracts was also determined. The two cyanobacterial strains were identified as Thermoleptolyngbya sp. and Leptolyngbya sp. and the molecular analysis also confirmed the identity of pathogenic bacteria. The untreated cyanobacterial aqueous extracts had little effect against the two bacterial strains. In contrast, the extract directly exposed to the laser was significantly more effective, with an inhibition zone of 22.0 mm in the case of a time of 32 min and distance of 10 cm against S. aureus. Accessory pigment composition increased in extracts directly exposed to the laser. This is the first case report on the effect of lasers on enhancing the antimicrobial profile of cyanobacterial extracts against SA and MRSA bacterial pathogens, as well as enhancing accessory pigment content. The laser dose that was most effective was that of 32 min time and 10 cm distance of Thermoleptolyngbya sp. extract directly exposed to the laser, which highlights the importance of time for increasing the laser dose and consequently increasing its antimicrobial impact.
Integrated Patterns of Subjective Job Insecurity: A Multigroup Person-Centered Study
Authors: Ghezzi Valerio , Ciampa Valeria , Probst Tahira M. , Petitta Laura , Marzocchi Ivan , Olivo Ilaria , Barbaranelli Claudio
Published: October 2022

Past research attests to the pivotal role of subjective job insecurity (JI) as a major stressor within the workplace. However, most of this research has used a variable-centered approach to evaluate the relative importance of one (or more) JI facets in explaining employee physical and psychological well-being. Relatively few studies have adopted a person-centered approach to investigate how different appraisals of JI co-occur within employees and how these might lead to the emergence of distinct latent profiles of JI, and, moreover, how those profiles might covary with well-being, personal resources, and performance. Using conservation of resources (COR) theory as our overarching theoretical framework and latent profile analysis as our methodological approach, we sought to fill this gap. To evaluate the external validity of our study results, we used employee sample data from two different countries (Italy and the USA) with, respectively, n = 743 and n = 494 employees. Results suggested the emergence of three profiles (i.e., the “secure”, the “average type”, and the “insecure”) in both country samples. The “secure” group systematically displayed a less vulnerable profile in terms of physical and psychological well-being, self-rated job performance, positive orientation, and self-efficacy beliefs than the “insecure” group, while the “average” type position on the outcomes’ continua was narrower. Theoretically, this supports COR’s notion of loss spirals by suggesting that differing forms of JI appraisals tend to covary within-person. Practical implications in light of labor market trends and the COVID-19 pandemic are discussed.
Czech Consumers’ Preference for Organic Products in Online Grocery Stores during the COVID-19 Pandemic
Authors: Zámková Martina , Rojík Stanislav , Prokop Martin , Činčalová Simona , Stolín Radek
Published: October 2022

A major advantage of online organic produce shopping is the fact that it saves energy and reduces emissions otherwise generated by customers during their time spent on the road and while shopping. Organic products in general positively impact sustainability, the environment, and the regions of their origin along with the social changes in these regions and further rural development. Moreover, these products positively impact the perceived health benefits and quality of food labeled as organic. The Czech Republic has currently seen a rise in organic food purchasing and supply trends. This study maps the factors possibly influencing consumers’ decision to go shopping for organic food online. Observed factors include the following demographic characteristics of consumers (respondents): gender, age, education, household income, number of children in the household and number of household members. A total of 757 respondents from the Czech Republic from September 2020 to December 2020 took part in the research. Logistic regression, used for data processing, identified the statistically significant effects of education, income and number of household members on online purchases. These conclusions were confirmed by a detailed contingency tables analysis, including the almost monotonous trend of the dependencies, with only minor deviations in a maximum of one category. The strongest influence of some categories on the emergence of partial dependencies was found by residue analysis. The research confirmed that the frequency of online grocery shopping increases significantly with increasing education and income of respondents and decreases with increasing the number of household members. Most respondents apparently shop for groceries online because of time savings, better product choice and more convenient and easier search.
Digital Transformation and Green Innovation of Chinese Firms: The Moderating Role of Regulatory Pressure and International Opportunities
Authors: He Jinqiu , Su Huiwen
Published: October 2022

The digitalization of business processes has increasingly challenged conventional wisdom in corporate green innovation. This empirical paper studies the timely but theoretically underexplored relationship between digital transformation and green innovation in a developing country context. Given that firms’ digital transformation shifts organizational structures toward decentralization, we employ a digital perspective to analyze organizational coordination, control, and learning mechanisms and propose that digital transformation positively affects corporate green innovation. Moreover, drawing on structural contingency theory, we demonstrate that such effects can be strengthened by external contingencies, specifically regulatory pressure and international opportunities. Using a dataset of Chinese listed firms, we find empirical support for our hypotheses. Our study is one of the first to examine how firms can leverage organizational digital transformation to enhance their green innovation performance and thus provides new insights into the drivers of sustainable practices for firms in developing countries.
How Does Internet Use Improve Mental Health among Middle-Aged and Elderly People in Rural Areas in China? A Quasi-Natural Experiment Based on the China Health and Retirement Longitudinal Study (CHARLS)
Authors: Fan Shishuai , Yang Yifan
Published: October 2022

One of the most significant public health issues in rural China is how to improve the mental health of middle-aged and older individuals. Using 2013, 2015, and 2018 CHARLS panel data, this paper properly examined the effects of Internet use on the mental health of middle-aged and elderly people in rural China based on the difference-in-differences method. The findings are as follows: (1) Internet use effectively improves the mental health status of middle-aged and elderly people in rural China; (2) compared to the middle-aged group, Internet use has a more obvious effect on the mental health of the elderly; (3) further analysis showed that reading news, watching videos, and playing games online could significantly improve the mental health status of middle-aged and elderly people in rural China, while chatting online and other Internet activities cannot significantly improve mental health status; and (4) playing games, watching videos, and reading news have different effects on the mental health of middle-aged and elderly people in rural China. The results indicate that playing games have a better effect on depression levels than watching videos. In contrast, watching news had the lowest effect on depression levels among middle-aged and elderly people in rural China. The results of this study also show the latest evidence that Internet use can help China’s rural middle-aged and elderly populations to reduce social isolation, establish new social connections, gain social support, and, ultimately, achieve active ageing. Therefore, promoting multiple forms of interaction is an effective path to prevent loneliness, which has become the new policy direction of the government to create an age-friendly Internet environment using various measures in the future to eliminate the barriers to Internet access affecting the middle-aged and elderly in rural China.
Acute Gaseous Air Pollution Exposure and Hospitalizations for Acute Ischemic Stroke: A Time-Series Analysis in Tianjin, China
Authors: Cui Mingrui , Zhan Changqing , Wu Wenjuan , Guo Dandan , Song Yijun
Published: October 2022

Background: Stroke has always been an important problem troubling human health. Short-term exposure to air pollutants is associated with increased hospital admissions. The rise of pollutants such as O3 has caused a huge social and economic burden. This study aims to explore the relationship between short-term exposure to ambient gaseous pollutants and daily hospitalizations for ischemic stroke, utilizing a four-year time-series study in Tianjin. Methods: Collecting the data of gaseous pollutants (NO2, SO2, CO, O3), meteorological data (including daily average temperature and relative humidity) and the number of hospitalizations due to ischemic stroke in Tianjin Medical University General Hospital from 2013 to 2016. Poisson regression generalized additive model with single-day and multi-day moving average lag structure was used to estimate adverse effects of gaseous pollutants on daily hospitalizations. Subgroup analysis was performed to detect modification effect by gender and age. Results: In total, there were 9081 ischemic stroke hospitalizations. After controlling for the meteorological factors in the same period, no significant findings were found with the increase of NO2, SO2, CO and O3 concentrations at most of the time in the single-pollutant model. Similarly, in the stratified analysis, no associations between gaseous pollutants and ischemic stroke were observed in this study. Conclusions: Short-term exposure to NO2, SO2, CO and O3 was not distinctly associated with daily hospitalizations for ischemic stroke in Tianjin. Multicenter studies in the future are warranted to explore the associations between gaseous pollution exposure and ischemic stroke.
Targeted Telehealth Education Increases Interest in Using Telehealth among a Diverse Group of Low-Income Older Adults
Authors: Jezewski Emily , Miller Abigale , Eusebio MaryAnn , Potter Jane
Published: October 2022

Telehealth allows older adults to take control over their health and preventive care; however, they are less likely to use telehealth. Minority older adults use telehealth services less than their White counterparts. During COVID-19, the U.S. Medicare system allowed for telehealth delivery of Annual Wellness Visits, which are known to improve use of preventive services. To increase telehealth use, we targeted vulnerable, low-income, minority older adults and provided education to improve knowledge of and identify barriers to telehealth use. Ultimately, this could serve as a means of improving health and preventive care services. Participants resided at independent living facilities, low-income housing, and elders of the Native American coalition; N = 257. Participants received written education materials; a subset attended a 20-min presentation. In this quasi-experimental study, participants completed a pre-post survey. Results were analyzed using Chi-Squared and Fisher’s Exact tests. Participants included 54 ‘in-person’ and 203 ‘at-home’ learners. Most were female (79%), single/widowed (51%), and white (65%). At baseline, 39% were familiar with telehealth; following education 73% stated understanding on accessing telehealth. Nearly 40% of participants said they would use telehealth in the future; a larger proportion of “in-person” (73%) learners were willing to use telehealth than “at-home” learners (41%) (p = 0.001). Divorced older adults and Blacks voiced greater likelihoods of using telehealth than their married/widowed and White counterparts, respectively (Χ2(3, N = 195) = 9.693, p = 0.02), (p = 0.01). This education program demonstrates an increase likelihood in health promotion among older adults by increasing confidence in accessing and future use of telehealth; therefore, we achieved our aim of promoting telehealth use and improving health promotion.
Study on the Relationship between Nurses’ Mentoring Relationship and Organizational Commitment
Authors: Gong Zhenxing , Van Swol Lyn M. , Wang Xiangge
Published: October 2022

The mentoring relationship affects the growth and development of new employees. For nurses, the uncertainty of the influence of the mentoring relationship may be magnified by the unique nature of hospitals as public departments, however it is unclear whether and how nurses’ mentoring relationship influence the outcome. Protean career orientation defined as a tendency of individuals to achieve subjective career success through self-management of their career is crucial to the influence mechanism of the mentoring relationship. The aim of this study was to explore the path and boundary conditions of the influence of the nurses’ mentoring relationship on organizational commitment. As a cross-sectional sample, 371 nurses were investigated. The results showed that protégé career optimism plays an intermediary role in the influence of the mentoring relationship on organizational commitment, and protean career orientation plays a moderating role in the influence of the mentoring relationship on career optimism. The mentor relationship between mentors and protégés facilitates protégés’ career optimism, enhancing the protégés’ organizational commitment, especially for protégés with low protean career orientation. These findings contribute to the improving nurses’ organizational commitment through mentoring relationship. Hospitals should provide space for nurses to exert their abilities, enhance opportunities to improve their team cooperation ability, clearly define the scope of nurses’ work and rights, and give nurses the right to make decisions.
Analysis of UNESCO ESD Priority Areas’ Implementation in Romanian HEIs
Authors: Dumitrescu Corina-Ionela , Moiceanu Georgiana , Dobrescu Razvan-Mihai , Popescu Mirona Ana Maria
Published: October 2022

Higher education institutions (HEIs) are adopting sustainable development (SD) in their strategies for the future. The roadmap by UNESCO is the path to follow to reach success. The approach is different for every HEI, thus the objective of this paper is to analyze the current state of education for sustainable development activities provided by HEIs through the eyes of the academic community (responders category: professors, researchers, associate professors). The method to conduct the study was an interview that had 40 enclosed questions and a free part at the end where the responders could bring additional information to the study if they considered it necessary. All the interviews were transcribed and given a code (e.g., RHEI1, RHEI 29) in order to perform the analysis using descriptive statistics with the help of the program MS Office EXCEL. The results showed some areas where the activities provided by HEIs need improvement and also revealed promising aspects through partnerships. Making the values of SD known to the academic and local community will help fulfill the true potential for change and future development. Moreover, the analysis showed the need to educate educators and improve their digital skills and teaching methods/techniques in order to achieve sustainable development. Another result revealed the need for improvements in HEI curricula that will contribute to gaining those skills/abilities that emerging jobs should have.
The Relationship between Personality Traits and Facebook Addiction among Adolescents in an Urban, Rural and Semi-Rural Secondary School
Authors: Maepa Mokoena Patronella , Wheeler Alicia
Published: October 2022

Facebook is and was intended to provide a place for friends to connect within the bustling academic environment and to encourage openness for ideas and interests. When used sparingly, it can provide an individual with a sense of group belonging and connection, sharing and offering hope and advice. The misuse of Facebook can have detrimental effects on one’s quality of life that often lead to addiction. In this correlation design study, secondary-school-aged adolescents’ Facebook addiction was compared to personality attributes. Through a convenience sample, 240 teenagers in total (106 men and 134 females) were chosen. The respondents answered questions about their demographics, Facebook Addiction, and Junior Eysenck Personality. The findings showed a substantial inverse correlation between Facebook addiction and neuroticism (r = −0.260, p < 0.01) and psychoticism (r = −0.189, p < 0.01). There was no discernible statistical link between Facebook Addiction and Extraversion. The study comes to the conclusion that although social networking sites such as Facebook have good effects on adolescents’ lives, their use needs to be regulated, the risks were highlighted, and at-risk individuals can receive intervention approaches, such as social skills training.
Clinical Field and Alternative Clinical Practice Experience in a Pandemic Situation of Nursing Students Who Have Experienced Clinical Practice before COVID-19
Authors: An Hyeran , Park Sunnam , Lee Jongeun
Published: October 2022

This study aimed to understand the experiences of nursing students who experienced clinical practice before the outbreak of COVID-19 on clinical field practice and alternative clinical practice adopted during the COVID-19 pandemic. A phenomenological study was conducted on 14 graduates who experienced clinical field practice and alternative clinical practice during a pandemic. Data were collected using individual in-depth interviews that were semi-structured. Data were analyzed according to Colaizzi’s procedure. As a result of data analysis, five themes and 17 sub-themes were selected. The five themes were: alienation during the process of clinical practice change, regret caused by alternative clinical practice, alternative clinical practice as a supplementary measure, difficulties due to COVID-19, non-replaceable clinical field practice. It is necessary to consider using alternative clinical practices to complement the clinical setting and improve the quality of clinical practice in the post-corona era. To this end, it is necessary to supplement the disadvantages of alternative clinical practice, such as reduced concentration and lack of sense of presence, by applying integrated education using Edutech.
Associations between Lifestyle Changes, Risk Perception and Anxiety during COVID-19 Lockdowns: A Case Study in Xi’an
Authors: Yang Huan , Zhao Qingyun , Zhang Zhengkai , Jia Wenxiao
Published: October 2022

The outbreak of COVID-19 dramatically changed individuals’ lifestyles, which in turn triggered psychological stress and anxiety. Many previous studies have discussed the relationships between lifestyle changes and anxiety and risk perception and anxiety independently. However, few papers have discussed these factors in a comprehensive and systematic manner. We established a six-dimensional system to assess changes in individuals’ lifestyles, which include dietary habits, physical activity (PA), sleep, screen time, smoking and alcohol consumption, and interaction with neighbors. Then, we collected information relating to socio-demographics, lifestyle changes, risk perception, and anxiety, and discussed their associations using multilinear and stepwise logistic regressions. The results show that not all lifestyle changes had an influence on anxiety. Changes in PA and interaction with neighbors were not significantly associated with anxiety. Risk perception was found to be inversely related to anxiety. Changes in dietary habits, family harmony, and net income were negatively related to anxiety among the group with higher risk perception. As individuals perceived a higher severity of COVID-19, the impact of their financial status on anxiety increased. These findings provide a valuable resource for local governments seeking to refine their pandemic strategies by including approaches such as advocating healthy lifestyles and stabilizing the job market to improve individuals’ mental health during lockdowns.
Multifractal Correlation between Terrain and River Network Structure in the Yellow River Basin, China
Authors: Qin Zilong , Wang Jinxin
Published: October 2022

As the most basic physical geographic elements, basin terrain and river networks have high spatial complexity and are closely related. However, there is little research on the correlation between terrain and river networks. In this paper, the Yellow River Basin was selected as the study area. Topographic factors of multiple dimensions were calculated. The influence of different topographic factors on the river network structure at different scales and their correlation from a multifractal perspective based on geographical detectors and a geographically weighted regression model were determined. The explanatory power of topography on the river network structure at different scales was: multifractal spectrum width > multifractal spectrum difference > slope > average elevation > elevation maximum > elevation minimum, which generally indicated that the topographic factor that has the greatest influence on the river network structure is the complexity and singularity of the terrain. The second-order clustering of regression coefficients from the results of the geographically weighted regression model revealed that the Yellow River basin was divided into three types of high-aggregation areas, which are dominated by the Qinghai-Tibet Plateau, the Loess Plateau, and the Huang-Huaihai Plain, respectively. The clustering results also revealed that the river network structure was affected by different key topographic factors in the different types of areas. This research studies and quantifies the relationship between basin topography and river network structure from a new perspective and provides a theoretical basis for unraveling the development of topography and river networks.
Vitamin C Suppresses Pancreatic Carcinogenesis through the Inhibition of Both Glucose Metabolism and Wnt Signaling
Authors: Kim Ji Hye , Hwang Sein , Lee Ji-Hye , Im Se Seul , Son Jaekyoung
Published: October 2022

Cumulative studies have indicated that high-dose vitamin C has antitumor effects against a variety of cancers. However, the molecular mechanisms underlying these inhibitory effects against tumorigenesis and metastasis, particularly in relation to pancreatic cancer, are unclear. Here, we report that vitamin C at high concentrations impairs the growth and survival of pancreatic ductal adenocarcinoma (PDAC) cells by inhibiting glucose metabolism. Vitamin C was also found to trigger apoptosis in a caspase-independent manner. We further demonstrate that it suppresses the invasion and metastasis of PDAC cells by inhibiting the Wnt/β-catenin-mediated epithelial-mesenchymal transition (EMT). Taken together, our results suggest that vitamin C has therapeutic effects against pancreatic cancer.
Proteomics Unveils Post-Mortem Changes in Beef Muscle Proteins and Provides Insight into Variations in Meat Quality Traits of Crossbred Young Steers and Heifers Raised in Feedlot
Authors: Severino Mariane , Gagaoua Mohammed , Baldassini Welder , Ribeiro Richard , Torrecilhas Juliana , Pereira Guilherme , Curi Rogério , Chardulo Luis Artur , Padilha Pedro , Neto Otávio Machado
Published: October 2022

Proteomics has been widely used to study muscle biology and meat quality traits from different species including beef. Beef proteomics studies allow a better understanding of the biological processes related to meat quality trait determination. This study aimed to decipher by means of two-dimensional electrophoresis (2D-PAGE), mass spectrometry and bioinformatics the changes in post-mortem muscle with a focus on proteins differentially expressed in the Longissimus thoracis (LT) muscle of immunocastrated young heifers and steers. Carcass traits, chemical composition, pH, instrumental color (L*, a*, b*), cooking loss and Warner-Bratzler shear force (WBSF) of meat from F1 Montana-Nellore cattle were also evaluated. Backfat thickness (BFT) and intramuscular fat content (IMF) were 46.8% and 63.6% higher in heifers (p < 0.05), respectively, while evaporation losses (EL) were 10.22% lower compared to steers. No differences (p > 0.05) were observed for tenderness evaluated by WBSF (3, 10, and 17 days post-mortem), pH, and color traits (L*, a* and b*) between the experimental groups. The study revealed several proteins to be differentially expressed proteins in heifers compared steers (p < 0.05). In heifers, proteins involved in nutrient transport (TF, ALB, and MB), energy metabolism (ALDOA, GAPDH, and PKM), and oxidative stress and response to stress (HSPA8 and CA3) were associated with a greater BFT and IMF deposition. The higher expression of these proteins indicated greater oxidative capacity and lower glycolytic activity in the LT muscle of heifers. In steers, there was greater abundance of protein expression related to muscle contraction and proteins of structure (ACTA1, TPM2 and TNNT3), energy metabolism (ENO1, ENO3, PYGM, PGM1 and TPI1) and ATP metabolism (ATP5F1B, PEBP1 and AK1), indicating greater glycogenolysis in LT muscle, suggesting a shift in the glycolytic/oxidative fibers of steers.
An Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation Method in a Soybean Root Biology Study
Authors: Huang Penghui , Lu Mingyang , Li Xiangbei , Sun Huiyu , Cheng Zhiyuan , Miao Yuchen , Fu Yongfu , Zhang Xiaomei
Published: October 2022

The stable genetic transformation of soybean is time-consuming and inefficient. As a simple and practical alternative method, hairy root transformation mediated by Agrobacterium rhizogenes is widely applied in studying root-specific processes, nodulation, biochemical and molecular functions of genes of interest, gene editing efficiency of CRISPR/Cas9, and biological reactors and producers. Therefore, many laboratories have developed unique protocols to obtain hairy roots in composite plants composed of transgenic roots and wild-type shoots. However, these protocols still suffer from the shortcomings of low efficiency and time, space, and cost consumption. To address this issue, we developed a new protocol efficient regeneration and transformation of hairy roots (eR&T) in soybean, by integrating and optimizing the main current methods to achieve high efficiency in both hairy root regeneration and transformation within a shorter period and using less space. By this eR&T method, we obtained 100% regeneration of hairy roots for all explants, with an average 63.7% of transformation frequency, which promoted the simultaneous and comparative analysis of the function of several genes. The eR&T was experimentally verified Promoter:GUS reporters, protein subcellular localization, and CRISPR/Cas9 gene editing experiments. Employing this approach, we identified several novel potential regulators of nodulation, and nucleoporins of the Nup107-160 sub-complex, which showed development-dependent and tissue-dependent expression patterns, indicating their important roles in nodulation in soybean. Thus, the new eR&T method is an efficient and economical approach for investigating not only root and nodule biology, but also gene function.
Neutralizing Ability of a Single Domain VNAR Antibody: In Vitro Neutralization of SARS-CoV-2 Variants of Concern
Authors: Valdovino-Navarro Blanca J. , Dueñas Salvador , Flores-Acosta G. Isaí , Gasperin-Bulbarela Jahaziel , Bernaldez-Sarabia Johanna , Cabanillas-Bernal Olivia , Cervantes-Luevano Karla E. , Licea-Navarro Alexei F.
Published: October 2022

Severe Acute Respiratory Syndrome Coronavirus 2 is the causal pathogen of coronavirus disease 2019 (COVID-19). The emergence of new variants with different mutational patterns has limited the therapeutic options available and complicated the development of effective neutralizing antibodies targeting the spike (S) protein. Variable New Antigen Receptors (VNARs) constitute a neutralizing antibody technology that has been introduced into the list of possible therapeutic options against SARS-CoV-2. The unique qualities of VNARs, such as high affinities for target molecules, capacity for paratope reformatting, and relatively high stability, make them attractive molecules to counteract the emerging SARS-CoV-2 variants. In this study, we characterized a VNAR antibody (SP240) that was isolated from a synthetic phage library of VNAR domains. In the phage display, a plasma with high antibody titers against SARS-CoV-2 was used to selectively displace the VNAR antibodies bound to the antigen SARS-CoV-2 receptor binding domain (RBD). In silico data suggested that the SP240 binding epitopes are located within the ACE2 binding interface. The neutralizing ability of SP240 was tested against live Delta and Omicron SARS-CoV-2 variants and was found to clear the infection of both variants in the lung cell line A549-ACE2-TMPRSS2. This study highlights the potential of VNARs to act as neutralizing antibodies against emerging SARS-CoV-2 variants.
Lysyl Oxidase Family Proteins: Prospective Therapeutic Targets in Cancer
Authors: Wang Wei , Wang Xiangjun , Yao Feng , Huang Chao
Published: October 2022

The lysyl oxidase (LOX) family, consisting of LOX and LOX-like proteins 1–4 (LOXL1–4), is responsible for the covalent crosslinking of collagen and elastin, thus maintaining the stability of the extracellular matrix (ECM) and functioning in maintaining connective tissue function, embryonic development, and wound healing. Recent studies have found the aberrant expression or activity of the LOX family occurs in various types of cancer. It has been proved that the LOX family mainly performs tumor microenvironment (TME) remodeling function and is extensively involved in tumor invasion and metastasis, immunomodulation, proliferation, apoptosis, etc. With relevant translational research in progress, the LOX family is expected to be an effective target for tumor therapy. Here, we review the research progress of the LOX family in tumor progression and therapy to provide novel insights for future exploration of relevant tumor mechanism and new therapeutic targets.
Lynch Syndrome Identification in Saudi Cohort of Endometrial Cancer Patients Screened by Universal Approach
Authors: Bu Rong , Siraj Abdul K. , Parvathareddy Sandeep Kumar , Iqbal Kaleem , Azam Saud , Qadri Zeeshan , Al-Rasheed Maha , Haqawi Wael , Diaz Mark , Alobaisi Khadija , Annaiyappanaidu Padmanaban , Siraj Nabil , AlHusaini Hamed , Alomar Osama , Al-Badawi Ismail A. , Al-Dayel Fouad , Al-Kuraya Khawla S.
Published: October 2022

Lynch syndrome (LS) is the most common cause of inherited endometrial cancer (EC). The prevalence and molecular characteristic of LS in Middle Eastern women with EC have been underexplored. To evaluate the frequency of LS in a cohort of EC patients from Saudi Arabia, a total of 436 EC cases were screened utilizing immunohistochemistry (IHC), MLH1 promoter methylation analysis and next-generation sequencing technology. A total of 53 of 436 (12.2%) ECs were classified as DNA mismatch repair-deficient (dMMR). MLH1 promoter hypermethylation was detected in 30 ECs (6.9%). Three ECs (0.7%) were found to be LS harboring germline pathogenic variants (PVs)/likely pathogenic variants (LPVs): two in the MSH2 gene and one in the MSH6 gene. Three ECs (0.7%) were Lynch-like syndrome (LLS) carrying double somatic MSH2 PVs/LPVs. Seven cases were found to have variants of uncertain significance in cancer-related genes other than MMR genes. Our results indicate that LS prevalence is low among Saudi EC patients and LLS is as common as LS in this ethnicity. Our findings could help in better understanding of the prevalence and mutational spectrum of this syndrome in Saudi Arabia, which may help in defining best strategies for LS identification, prevention and genetic counseling for EC patients.