bluuebunny/crossref_metadata_2025 huggingface dataset:
Chemical Composition and Bioactive Properties of Commercial and Non-Commercial Purple and White Açaí Berries
Authors: Matta Fernanda V. , Xiong Jia , Lila Mary Ann , Ward Neil I. , Felipe-Sotelo Mónica , Esposito Debora
Published: October 2020

Chemical composition analysis of açaí extracts revealed higher levels of total polyphenol content in purple açaí samples for both commercial (4.3–44.7 gallic acid equivalents mg/g) and non-commercial samples (30.2–42.0 mg/g) compared to white (8.2–11.9 mg/g) and oil samples (0.8–4.6 mg/g). The major anthocyanin compounds found in purple açaí samples were cyanidin-3-glucoside and cyanidin-3-rutinoside with total concentrations in the range of 3.6–14.3 cyanidin-3-glucoside equivalents mg/g. The oligomeric proanthocyanidins were quantified in the range of 1.5–6.1 procyanidin B1 equivalents mg/g. Moreover, açaí presented significant levels of calcium, magnesium, manganese, iron, zinc and copper, essential minor and trace elements, in comparison with other berries. All of the açaí extracts at 50 μg/mL potently inhibited the release of reactive oxygen species in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells, but none inhibited the release of nitric oxide. Furthermore, all the açaí samples demonstrated potential as wound healing agents due to the high levels of migration activity in human fibroblast cells.
Vitamin D Intake in a Population-Based Sample of Young Polish Women, Its Major Sources and the Possibility of Meeting the Recommendations
Authors: Utri Zofia , Głąbska Dominika
Published: October 2020

The recommendations of vitamin D intake are commonly not met, which results from the fact that fish, being its major sources, are commonly rarely consumed. Consequently, a reliable estimation of its habitual intake is also difficult, as its daily intake is highly variable. The aim of the study was to analyze vitamin D intake from food, its major sources and the possibility to meet its recommendations in a population-based sample of young Polish women. The study was conducted in a sample of Polish women aged 15–30 years, recruited in cooperation with local students’ and youth organizations from all regions of Poland (convenience sampling with the snowball effect), while the stratified sampling procedure was applied with a random quota sampling for voivodeships (an administrative subdivision), to obtain an adequate distribution regarding the general population of young Polish women (n = 1,032). The vitamin D intake was assessed while using the validated Vitamin D Estimation Only—Food Frequency Questionnaire (VIDEO-FFQ) and was compared with the recommended 10 µg. The median vitamin D intake in the study group was 3.09 µg (0.00–24.52 µg) and in 95% of participants was lower than recommended, while the highest vitamin D intake was observed for the following sources: eggs (0.50 µg), meat and meat products (0.49 µg), herring, sardine and tuna products (0.41 µg) and dairy products (0.40 µg). The correlation between total vitamin D intake and its intake from its sources was strongest for eggs (p < 0.0001; R = 0.5989) and for herring, sardine and tuna products (p < 0.0001; R = 0.5314), while the correlation between total vitamin D intake and the number of servings was strongest for herring, sardine and tuna products (p < 0.0001; R = 0.5314). At the same time, while compared with other fish species, consuming herring was the strongest predictor of meeting the recommended vitamin D level of 10 µg (p = 0.0292; odds ratio (OR) = 1.94; 95% confidence interval (CI) 1.07–3.52), but also of 5 µg (p < 0.0001; OR = 2.54; 95% CI 1.85–3.47). Therefore, taking into account the relatively low prices of herring, its high vitamin D content, as well as its influence on total vitamin D intake, it could be beneficial to recommend young women to increase herring intake in order to increase dietary vitamin D intake and to meet its recommendations.
A Hybrid Method for the Run-Of-The-River Hydroelectric Power Plant Energy Forecast: HYPE Hydrological Model and Neural Network
Authors: Ogliari Emanuele , Nespoli Alfredo , Mussetta Marco , Pretto Silvia , Zimbardo Andrea , Bonfanti Nicholas , Aufiero Manuele
Published: October 2020

The increasing penetration of non-programmable renewable energy sources (RES) is enforcing the need for accurate power production forecasts. In the category of hydroelectric plants, Run of the River (RoR) plants belong to the class of non-programmable RES. Data-driven models are nowadays the most widely adopted methodologies in hydropower forecast. Among all, the Artificial Neural Network (ANN) proved to be highly successful in production forecast. Widely adopted and equally important for hydropower generation forecast is the HYdrological Predictions for the Environment (HYPE), a semi-distributed hydrological Rainfall–Runoff model. A novel hybrid method, providing HYPE sub-basins flow computation as input to an ANN, is here introduced and tested both with and without the adoption of a decomposition approach. In the former case, two ANNs are trained to forecast the trend and the residual of the production, respectively, to be then summed up to the previously extracted seasonality component and get the power forecast. These results have been compared to those obtained from the adoption of a ANN with rainfalls in input, again with and without decomposition approach. The methods have been assessed by forecasting the Run-of-the-River hydroelectric power plant energy for the year 2017. Besides, the forecasts of 15 power plants output have been fairly compared in order to identify the most accurate forecasting technique. The here proposed hybrid method (HYPE and ANN) has shown to be the most accurate in all the considered study cases.
Cost Estimating Using a New Learning Curve Theory for Non-Constant Production Rates
Authors: Hogan Dakotah , Elshaw John , Koschnick Clay , Ritschel Jonathan , Badiru Adedeji , Valentine Shawn
Published: October 2020

Traditional learning curve theory assumes a constant learning rate regardless of the number of units produced. However, a collection of theoretical and empirical evidence indicates that learning rates decrease as more units are produced in some cases. These diminishing learning rates cause traditional learning curves to underestimate required resources, potentially resulting in cost overruns. A diminishing learning rate model, namely Boone’s learning curve, was recently developed to model this phenomenon. This research confirms that Boone’s learning curve systematically reduced error in modeling observed learning curves using production data from 169 Department of Defense end-items. However, high amounts of variability in error reduction precluded concluding the degree to which Boone’s learning curve reduced error on average. This research further justifies the necessity of a diminishing learning rate forecasting model and assesses a potential solution to model diminishing learning rates.
Market Power in Output and Emissions Trading
Authors: André Francisco J. , de Castro Luis Miguel
Published: October 2020

This article focuses on the strategic behavior of firms in the output and the emissions markets in the presence of market power. We consider the existence of a dominant firm in the permit market and different structures in the output market, including Cournot and two versions of the Stackelberg model, depending on whether the permit dominant firm is a leader or a follower in the output market. In all three models, the firm that dominates the permit market is more sensitive to its initial allocation than its competitor in terms of abatement and less sensitive in terms of output. In all three models, output is decreasing and the permit price is increasing in the permit dominant firm’s initial allocation. In the Cournot model, permit dominance is fruitless in terms of output and profit if the initial allocation is symmetric. Output leadership is more relevant than permit dominance since an output leader always tends to, ceteris paribus, produce more and make more profit whether it also dominates the permit market or not. This leadership can only be overcompensated for by distributing a larger share of permits to the output follower, and only if the total number of permits is large enough. In terms of welfare, Stackelberg is always superior to Cournot. If the initial permit allocation is symmetric, welfare is higher when the same firm dominates the output and the permit market at the same time.
Cooperation between Emotional Players
Authors: Andersson Lina
Published: October 2020

This paper uses the framework of stochastic games to propose a model of emotions in repeated interactions. An emotional player can be in either a friendly, a neutral, or a hostile state of mind. The player transitions between the states of mind as a response to observed actions taken by the other player. The state of mind determines the player’s psychological payoff which together with a material payoff constitutes the player’s utility. In the friendly (hostile) state of mind the player has a positive (negative) concern for other players’ material payoffs. This paper shows how emotions can both facilitate and obstruct cooperation in a repeated prisoners’ dilemma game. In finitely repeated games a player who cares only for their own material payoffs can have an incentive to manipulate an emotional player into the friendly state of mind. In infinitely repeated games with two emotional players less patience is required to sustain cooperation. However, emotions can also obstruct cooperation if they make the players unwilling to punish each other, or if the players become hostile when punished.
Fenugreek Stimulates the Expression of Genes Involved in Milk Synthesis and Milk Flow through Modulation of Insulin/GH/IGF-1 Axis and Oxytocin Secretion
Authors: Sevrin Thomas , Boquien Clair-Yves , Gandon Alexis , Grit Isabelle , de Coppet Pierre , Darmaun Dominique , Alexandre-Gouabau Marie-Cécile
Published: October 2020

We previously demonstrated galactagogue effect of fenugreek in a rat model of lactation challenge, foreshadowing its use in women’s breastfeeding management. To assess longitudinal molecular mechanisms involved in milk synthesis/secretion in dams submitted to fenugreek supplementation, inguinal mammary, pituitary glands and plasma were isolated in forty-three rats nursing large 12 pups-litters and assigned to either a control (CTL) or a fenugreek-supplemented (FEN) diet during lactation. RT-PCR were performed at days 12 and 18 of lactation (L12 and L18) and the first day of involution (Inv1) to measure the relative expression of genes related to both milk synthesis and its regulation in the mammary gland and lactogenic hormones in the pituitary gland. Plasma hormone concentrations were measured by ELISA. FEN diet induced 2- to 3-times higher fold change in relative expression of several genes related to macronutrient synthesis (Fasn, Acaca, Fabp3, B4galt1, Lalba and Csn2) and energy metabolism (Cpt1a, Acads) and in IGF-1 receptor in mammary gland, mainly at L12. Pituitary oxytocin expression and plasma insulin concentration (+77.1%) were also significantly increased. Altogether, these findings suggest fenugreek might extend duration of peak milk synthesis through modulation of the insulin/GH/IGF-1 axis and increase milk ejection by activation of oxytocin secretion.
A Case Report of Left Atrial Isomerism in a Syndromic Context
Authors: Ilian Aurora , Motoc Andrei , Balulescu Ligia , Secosan Cristina , Grigoras Dorin , Pirtea Laurentiu
Published: October 2020

The objective of our paper is to underline the importance of assessing microarray genetic analysis for the detection of chromosomal abnormalities in rare cases such as left atrial isomerism, mostly in the context of antenatally detected syndromes. We present the case of a 26-year-old primipara, at 26 weeks of gestation, with prior first trimester normal anomaly scan, who presented in our department accusing lower abdominal pain. An anomaly ultrasound examination of the fetus revealed cardiomegaly with increased size of the right atrium, non-visualization of the atrial septum or the foramen ovale, malalignment of the three-vessel view, location of the superior vena cava above the two-vessel view, slight pericardial effusion, and no interruption of the inferior vena cava nor presence of azygos vein being noted. Associated extracardiac abnormalities, such as small kidneys at the level of the iliac fossa, micrognathia, dolichocephaly with hypoplasia of the cerebellum, increased nuchal fold, and reduced fetal movements were also reported. A diagnostic amniocentesis was performed, and, while the conventional rapid prenatal diagnostic test of the multiplex quantitative fluorescent polymerase chain reaction (PCR) came as normal, the microarray analysis (ChAS, NCBI Built 37 hg 19, detection of microdeletions or microduplications larger than 100 kb) revealed two chromosomal abnormalities: a 22.84 Mb loss of genetic material in the 18q21.31–18q23 chromosomal region and a gain of 22.31 Mb of genetic material in the 20p13–20p11.21 chromosomal region. After the termination of pregnancy, a necropsy of the fetus was performed, confirming heterotaxy syndrome with a common atrium, no atrial septum, superior vena cava draining medianly, and pulmonary veins that drained into the lower segment of the left atrium due to an anatomically enlarged single common atrium. The extracardiac findings consisted of two bilobar lungs, dysmorphic facies, low-set ears, nuchal fold edema, and small kidneys located in the iliac fossa. These findings are conclusive evidence that left atrial isomerism is a more complex syndrome. The genetic tests of the parents did not reveal any translocations of chromosomes 18 and 20 when the Fluorescent in situ Hybridization (FISH) analysis was assessed. The antenatal detection of corroboration between different structural abnormalities using serial ultrasound examinations and cardiac abnormalities, together with the detection of the affected chromosomes, improves the genetic counseling regarding the prognosis of the fetus and the recurrence rate of the condition for siblings.
SLC19A3 Loss-of-Function Variant in Yorkshire Terriers with Leigh-Like Subacute Necrotizing Encephalopathy
Authors: Drögemüller Michaela , Letko Anna , Matiasek Kaspar , Jagannathan Vidhya , Corlazzoli Daniele , Rosati Marco , Jurina Konrad , Medl Susanne , Gödde Thomas , Rupp Stefan , Fischer Andrea , Luján Feliu-Pascual Alejandro , Drögemüller Cord
Published: October 2020

Sporadic occurrence of juvenile-onset necrotizing encephalopathy (SNE) has been previously reported in Yorkshire terriers. However, so far, no causative genetic variant has been found for this breed-specific form of suspected mitochondrial encephalomyopathy. Affected dogs showed gait abnormalities, central visual defects, and/or seizures. Histopathological analysis revealed the presence of major characteristics of human Leigh syndrome and SNE in Alaskan huskies. The aim of this study was to characterize the genetic etiology of SNE-affected purebred Yorkshire terriers. After SNP genotyping and subsequent homozygosity mapping, we identified a single loss-of-function variant by whole-genome sequencing in the canine SLC19A3 gene situated in a 1.7 Mb region of homozygosity on chromosome 25. All ten cases were homozygous carriers of a mutant allele, an indel variant in exon 2, that is predicted to lead to a frameshift and to truncate about 86% of the wild type coding sequence. This study reports a most likely pathogenic variant in SLC19A3 causing a form of SNE in Yorkshire terriers and enables selection against this fatal neurodegenerative recessive disorder. This is the second report of a pathogenic alteration of the SLC19A3 gene in dogs with SNE.
Comparative Study of Pine Reference Genomes Reveals Transposable Element Interconnected Gene Networks
Authors: Voronova Angelika , Rendón-Anaya Martha , Ingvarsson Pär , Kalendar Ruslan , Ruņģis Dainis
Published: October 2020

Sequencing the giga-genomes of several pine species has enabled comparative genomic analyses of these outcrossing tree species. Previous studies have revealed the wide distribution and extraordinary diversity of transposable elements (TEs) that occupy the large intergenic spaces in conifer genomes. In this study, we analyzed the distribution of TEs in gene regions of the assembled genomes of Pinus taeda and Pinus lambertiana using high-performance computing resources. The quality of draft genomes and the genome annotation have significant consequences for the investigation of TEs and these aspects are discussed. Several TE families frequently inserted into genes or their flanks were identified in both species’ genomes. Potentially important sequence motifs were identified in TEs that could bind additional regulatory factors, promoting gene network formation with faster or enhanced transcription initiation. Node genes that contain many TEs were observed in multiple potential transposable element-associated networks. This study demonstrated the increased accumulation of TEs in the introns of stress-responsive genes of pines and suggests the possibility of rewiring them into responsive networks and sub-networks interconnected with node genes containing multiple TEs. Many such regulatory influences could lead to the adaptive environmental response clines that are characteristic of naturally spread pine populations.
Point Mutations in the 14-α Sterol Demethylase Cyp51A or Cyp51C Could Contribute to Azole Resistance in Aspergillus flavus
Authors: Lucio Jose , Gonzalez-Jimenez Irene , Rivero-Menendez Olga , Alastruey-Izquierdo Ana , Pelaez Teresa , Alcazar-Fuoli Laura , Mellado Emilia
Published: October 2020

Infections caused by Aspergillus species are being increasingly reported. Aspergillus flavus is the second most common species within this genus causing invasive infections in humans, and isolates showing azole resistance have been recently described. A. flavus has three cyp51-related genes (cyp51A, cyp51B, and cyp51C) encoding 14-α sterol demethylase-like enzymes which are the target of azole drugs. In order to study triazole drug resistance in A. flavus, three strains showing reduced azole susceptibility and 17 azole susceptible isolates were compared. The three cyp51-related genes were amplified and sequenced. A comparison of the deduced Cyp51A, Cyp51B, and Cyp51C protein sequences with other protein sequences from orthologous genes in different filamentous fungi led to a protein identity that ranged from 50% to 80%. Cyp51A and Cyp51C presented several synonymous and non-synonymous point mutations among both susceptible and non-susceptible strains. However, two amino acid mutations were present only in two resistant isolates: one strain harbored a P214L substitution in Cyp51A, and another a H349R in Cyp51C that also showed an increase of cyp51A and cyp51C gene expression compared to the susceptible strain ATCC2004304. Isolates that showed reduced in vitro susceptibility to clinical azoles exhibited a different susceptibility profile to demethylation inhibitors (DMIs). Although P214L substitution might contribute to azole resistance, the role of H349R substitution together with changes in gene expression remains unclear.
Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital
Authors: Soliman May Sherif , Soliman Noha Salah , El-Manakhly Arwa Ramadan , ElBanna Shahira AbdelSalam , Aziz Ramy Karam , El-Kholy Amani Ali
Published: October 2020

Methicillin-resistant Staphylococcus aureus (MRSA) strains are associated with serious complications and poor clinical outcome. In Egypt, they contribute to more than 70% of S. aureus healthcare-associated infections. This study combined whole-genome sequencing, bioinformatics, and statistical analyses to identify the phylogeny, resistome, virulome and potential genotype–phenotype–clinical correlation among 18 clinical isolates of MRSA in a tertiary hospital in Cairo, Egypt. The ST1535-V MRSA clone was the most frequently isolated (16.6%), followed by ST5-VI, ST1-V and ST239-III (11.1% each). SCCmec V, VI, IV and III types were detected at frequencies of 50%, 16.6%, 11.1% and 11.1%, respectively. None of the tested virulence genes were detected in all isolates, but they ranged in distribution from 1/18 to 17/18. The Panton–Valentine leukocidin (PVL)-encoding genes were detected in only four isolates and were enriched in isolates causing non-severe cases. Phylogenetic analysis revealed relatedness between three ST1535-Vs, two ST5-VIs, two ST239-IIIs and two ST1-Vs; however, only the two genetically related ST1-V isolates were epidemiologically linked. While disease outcome and source of infection had no correlation with a particular genotypic pattern, the sequence type was the most correlated factor with phylogeny and genotypic patterns, and a few genes were associated with non-severe cases.
Validity of the GAITRite Walkway Compared to Functional Balance Tests for Fall Risk Assessment in Geriatric Outpatients
Authors: Riis Johannes , Byrgesen Stephanie M. , Kragholm Kristian H. , Mørch Marianne M. , Melgaard Dorte
Published: October 2020

This study examined the concurrent validity between gait parameters from the GAITRite walkway and functional balance test commonly used in fall risk assessment. Patients were sampled from one geriatric outpatient clinic. One physiotherapist evaluated the patients on the GAITRite walkway with three repetitions in both single- and dual-task conditions. Patients were further evaluated with Bergs Balance scale (BBS), Dynamic Gait index (DGI), Timed Up and Go (TUG), and Sit To Stand test (STS). Correlations between quantitative gait parameters and functional balance test were analyzed with Spearman’s rank correlations. Correlations strength was considered as follows: negligible <0.1, weak 0.10–0.39, moderate 0.40–0.69, and strong ≥0.70. We included 24 geriatric outpatients in the study with a mean age of 80.6 years (SD: 5.9). Patients received eight (SD: 4.5) different medications on average, and seven (29.2%) patients used walkers during ambulation. Correlations between quantitative gait parameters and functional balance test ranged from weak to moderate in both single- and dual-task conditions. Moderate correlations were observed for DGI, TUG, and BBS, while STS showed weak correlations with all GAITRite parameters. For outpatients analyzed on the GAITRite while using walkers, correlations showed no clear pattern across parameters with large variation within balance tests.
Suitability of Ultra-Short-Term Heart Rate Variability in Military Trainees
Authors: Alalyan Mubarak J. , Alkahtani Shaea A. , Habib Syed Shahid , Flatt Andrew A.
Published: October 2020

We aimed to (a) evaluate the agreement between ultra-short-term and criterion resting heart rate variability (HRV) measures in military trainees, and (b) compare associations between HRV recording lengths and body composition. HRV recordings were performed for 10 min in 27 military male students. Mean RR interval, the root-mean square of successive differences (RMSSD), RMSSD:RR interval ratio, standard deviation of normal-to-normal RR intervals (SDNN), and SDNN:RR interval ratio were determined from the last 5 min of the 10-min recording and considered the criterion. Parameters were also recorded in successive 1-min epochs from the 5-min stabilization period. No differences were observed between criterion values and any of the 1-min epochs (p > 0.05). Effect sizes ranged from −0.36–0.35. Intra-class correlations ranged from 0.83–0.99. Limits of agreement ranged from 38.3–78.4 ms for RR interval, 18.8–30.0 ms for RMSSD, 1.9–3.1 for RMSSD:RR, 24.1–31.4 ms for SDNN, and 2.5–3.0 for SDNN:RR. Body fat% was associated (p < 0.05) with all HRV parameters at varying time segments. A 1-min HRV recording preceded by a 1-min stabilization period seems to be a suitable alternative to criterion measures. Ultra-short procedures may facilitate routine HRV tracking in tactical populations for status-monitoring purposes.
Test–Retest Reliability of a Questionnaire on Motives for Physical Activity among Adolescents
Authors: Kopcakova Jaroslava , Dankulincova Veselska Zuzana , Kalman Michal , Filakovska Bobakova Daniela , Sigmundova Dagmar , Madarasova Geckova Andrea , Klein Daniel , van Dijk Jitse P. , Reijneveld Sijmen A.
Published: October 2020

The aim of this study was to investigate the test–retest reliability of the motives for undertaking physical activity (PA) items from the Health Behavior in School-Aged Children (HBSC) study questionnaire among Slovak and Czech adolescents and to determine whether this reliability differs by gender, age group and country. We obtained data from 580 students aged 11 and 15 years old (51.2% boys) who participated in a test and retest study with a four-week interval in 2013 via the Health Behavior in School-Aged Children cross-sectional study in the Czech Republic and Slovakia. We estimated the test–retest reliability of all 13 dichotomized motives by using Intraclass Correlation Coefficients (ICC) and Cohen’s Kappa statistics, for continuous and dichotomized motives, respectively. Test–retest reliability showed moderate agreement for nine motives (ICC from 0.41 to 0.60) and fair agreement for four motives (ICC from 0.33 to 0.40). Kappa statistics were similarly moderate to large (0.33 to 0.61), except for three motives with small or trivial correlations. The motives “To improve my health” and “To enjoy the feeling of using my body” had consistently low Kappas and correlations. Overall, the results of this study suggest that most questions on motives for PA on the HBSC questionnaire have acceptable test–retest characteristics for use among adolescents.
Translation and Validation of the Modified A-DIVA Scale to European Portuguese: Difficult Intravenous Access Scale for Adult Patients
Authors: Santos-Costa Paulo , Sousa Liliana B. , van Loon Fredericus H.J. , Salgueiro-Oliveira Anabela , Parreira Pedro , Vieira Margarida , Graveto João
Published: October 2020

(1) Background: In Portugal, no accurate and reliable predictive instruments are known that could assist healthcare professionals in recognizing patients with difficult venous access. Thus, this study aimed to translate and validate the Modified A-DIVA scale to European Portuguese. (2) Methods: A methodological and cross-sectional study was conducted in two phases: translation of the Modified A-DIVA scale to European Portuguese following six stages proposed by Beaton and collaborators, and assessment of its psychometric properties in a non-probability sample of 100 patients who required peripheral intravenous catheterization in a Portuguese hospital. (3) Results: The European version of the Modified A-DIVA scale (A-DM scale) showed excellent inter-rater accordance scores, k = 0.593 (95% CI, 0.847 to 0.970), p < 0.0005. The A-DM scale’s criterion and construct validity was assessed through predictive, convergent, and correlational analysis with variables identified in the literature as associated with difficult peripheral intravenous access, with moderate to large magnitudes and statistical significance. (4) Conclusions: The A-DM scale is a reliable and valid instrument that can support healthcare professionals and researchers in the early identification of patients at risk of difficult peripheral intravenous access. Future validation studies are needed to test the A-DM scale’s applicability across clinical settings and in different patient cohorts.
The Relationship of Urbanization and Performance of Activity and Participation Functioning among Adults with Developmental Disabilities in Taiwan
Authors: Lin Shyang-Woei , Chiu Tzu-Ying , Liou Tsan-Hon , Yen Chia-Feng , Chen Hui-Guan
Published: October 2020

Developmental disability is likely to be lifelong in nature and to result in substantial activity and societal participation limitations. The performance of individuals is related to the environment, supports, and urbanization of living cities. Most of the surveys for people with disabilities have not discussed the relationship between the cognitive impairment properties and performance of participation and activities functioning, and most cognitive impairments are regarded as having similar performance. The location of residence in childhood is mainly influenced by parents and main caregivers, but the factors related to the preferences of adults with cognitive impairment in the location of residence are more complicated. Objective(s): The aim was to explore and compare the relationships of the urbanization degree of their living cities and the functioning performance of daily living in various domains among adults with intellectual disability (ID), autism, and concomitant communicative impairment (CCI). Method: The cross-sectional study was applied, and the data was collected face-to-face by professionals in all authorized hospitals in Taiwan. The participants were 5374 adults with ID (n = 4455), autism (n = 670), CCI (n = 110) and combination disabilities (n = 139) which were according to the International Statistical Classification of Diseases 9th Revision (ICD-9) from a total of 167,069 adults with disabilities from the Disability Eligibility System (DES) in Taiwan between July 2012 and October 2013. The authors used the World Health Organization Disability Assessment Schedule 2.0–36 item version of WHO (WHODAS 2.0-36 items) to measure performance and capability of daily living. Results and Conclusions: There were significant differences in age, gender, disabled severity, and the urbanization between all subgroups (p < 0.05). After adjusting the age of all participators, the degree of urbanization just significantly affected the functioning score distribution in domain 1: cognition for an adult with ID, autism, and CCI; in domain 2, mobility for an adult with CCI and combination disability; in domain 3, self-care; domain 4, independent domains for ID (p < 0.05). There were no significant differences between urbanization degree and functioning scores in all domains for adults with autism. All in all, only in groups with combination disability did we find that the worse the degree of impairment was, the lower the degree of urbanization of their place of residence was, and there was no such phenomenon in adults with autism and ID in our study.
Bleeding Bodies, Untrustworthy Bodies: A Social Constructionist Approach to Health and Wellbeing of Young People in Kenya
Authors: Onyango Elizabeth Opiyo , Elliott Susan J.
Published: October 2020

The Sustainable Development Goals provide a global development agenda that is meant to be inclusive of all people. However, the development needs for vulnerable populations such as youth are not reflected within the policy agenda of some developing countries. One of the reasons for this is that research that explores health and wellbeing concerns for young people are sparse in the region and where they exist, the focus has been on marginalized subgroups. To address this gap, this cross-sectional study explored the health and wellbeing of youth in Kenya. We conducted 10 focus group discussions and 14 in-depth interviews with youth ages 15 to 24 years. A thematic analysis of the data revealed that structural factors are important influencers of youth perceptions and their social constructions of health and wellbeing. Kenyan youth are concerned about the health status and healthcare services in their communities, as well as issues of community trust of youths and perceived risks of political misuse and emotional suffering. Our findings suggest that youth transitioning into adulthood in resource-constrained areas experience feelings of powerlessness and inability to take charge over their own life. This impacts how they perceive and socially construct health and wellbeing.
Time between Symptom Onset, Hospitalisation and Recovery or Death: Statistical Analysis of Belgian COVID-19 Patients
Authors: Faes Christel , Abrams Steven , Van Beckhoven Dominique , Meyfroidt Geert , Vlieghe Erika , Hens Niel ,   Belgian Collaborative Group on COVID-19 Hospital Surveillance
Published: October 2020

There are different patterns in the COVID-19 outbreak in the general population and amongst nursing home patients. We investigate the time from symptom onset to diagnosis and hospitalization or the length of stay (LoS) in the hospital, and whether there are differences in the population. Sciensano collected information on 14,618 hospitalized patients with COVID-19 admissions from 114 Belgian hospitals between 14 March and 12 June 2020. The distributions of different event times for different patient groups are estimated accounting for interval censoring and right truncation of the time intervals. The time between symptom onset and hospitalization or diagnosis are similar, with median length between symptom onset and hospitalization ranging between 3 and 10.4 days, depending on the age of the patient (longest delay in age group 20–60 years) and whether or not the patient lives in a nursing home (additional 2 days for patients from nursing home). The median LoS in hospital varies between 3 and 10.4 days, with the LoS increasing with age. The hospital LoS for patients that recover is shorter for patients living in a nursing home, but the time to death is longer for these patients. Over the course of the first wave, the LoS has decreased.
Older Adults’ Gender, Age and Physical Activity Effects on Anxiety, Optimism, Resilience and Engagement
Authors: Martínez-Moreno Alfonso , Ibáñez-Pérez Ricardo José , Cavas-García Francisco , Cano-Noguera Francisco
Published: October 2020

The purpose of the study was to understand the effects of gender and age on anxiety, optimism, resilience and engagement in a group of older adults. An observational, quantitative, descriptive and transversal design was used with non-probabilistic sampling. Descriptive statistical analyses, reliability tests (Cronbach’s alpha) and linear correlation tests (Pearson’s) were performed, and the development of multivariate linear regression models was conducted. Female participants in the sample had higher levels in anxiety and pessimism, while male participants scored higher in optimism, engagement and resilience. Participants who practiced physical activity (PA) had better scores in optimism, engagement and resilience. The sample comprised 55.1% men and 44.9% women, between the ages of 51 and 93, with an average of 68.1 years, all participants completed the questionnaire Anxiety Scale-2 (SAS-2) the Revised Life Orientation Test (LOT-R) the short version of the Utrecht Work Engagement Scale questionnaire (UWES-9) the short version of the CD-RISC. As for marital status, there were significant differences between single participants and romantic partner. Singles participants showed higher levels of anxiety than their married counterparts, while those in a relationship scored higher in optimism, engagement and resilience. The model was statistically significant F (9;352) = 14.6; p < 0.001, explaining 27% of the variance in optimism. The data indicated that PA practice and living with a partner in an inland area is associated with less anxiety, which may have implications for programs and activities designed for older adults.
Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance
Authors: Joshi Abhayraj S. , Singh Priyanka , Mijakovic Ivan
Published: October 2020

Many bacteria have the capability to form a three-dimensional, strongly adherent network called ‘biofilm’. Biofilms provide adherence, resourcing nutrients and offer protection to bacterial cells. They are involved in pathogenesis, disease progression and resistance to almost all classical antibiotics. The need for new antimicrobial therapies has led to exploring applications of gold and silver nanoparticles against bacterial biofilms. These nanoparticles and their respective ions exert antimicrobial action by damaging the biofilm structure, biofilm components and hampering bacterial metabolism via various mechanisms. While exerting the antimicrobial activity, these nanoparticles approach the biofilm, penetrate it, migrate internally and interact with key components of biofilm such as polysaccharides, proteins, nucleic acids and lipids via electrostatic, hydrophobic, hydrogen-bonding, Van der Waals and ionic interactions. Few bacterial biofilms also show resistance to these nanoparticles through similar interactions. The nature of these interactions and overall antimicrobial effect depend on the physicochemical properties of biofilm and nanoparticles. Hence, study of these interactions and participating molecular players is of prime importance, with which one can modulate properties of nanoparticles to get maximal antibacterial effects against a wide spectrum of bacterial pathogens. This article provides a comprehensive review of research specifically directed to understand the molecular interactions of gold and silver nanoparticles with various bacterial biofilms.
Y-27632 Induces Neurite Outgrowth by Activating the NOX1-Mediated AKT and PAK1 Phosphorylation Cascades in PC12 Cells
Authors: Park So Yeong , An Jeong Mi , Seo Jeong Taeg , Seo Su Ryeon
Published: October 2020

Y-27632 is known as a selective Rho-associated coiled coil-forming kinase (ROCK) inhibitor. Y-27632 has been shown to induce neurite outgrowth in several neuronal cells. However, the precise molecular mechanisms linking neurite outgrowth to Y-27632 are not completely understood. In this study, we examined the ability of Y-27632 to induce neurite outgrowth in PC12 cells and evaluated the signaling cascade. The effect of Y-27632 on the neurite outgrowth was inhibited by reactive oxygen species (ROS) scavengers such as N-acetyl cysteine (NAC) and trolox. Furthermore, Y-27632-induced neurite outgrowth was not triggered by NADPH oxidase 1 (NOX1) knockdown or diphenyleneiodonium (DPI), a NOX inhibitor. Suppression of the Rho-family GTPase Rac1, which is under the negative control of ROCK, with expression of the dominant negative Rac1 mutant (Rac1N17) prevented Y-27632-induced neurite outgrowth. Moreover, the Rac1 inhibitor NSC23766 prevented Y-27632-induced AKT and p21-activated kinase 1 (PAK1) activation. AKT inhibition with MK2206 suppressed Y-27632-induced PAK1 phosphorylation and neurite outgrowth. In conclusion, our results suggest that Rac1/NOX1-dependent ROS generation and subsequent activation of the AKT/PAK1 cascade contribute to Y-27632-induced neurite outgrowth in PC12 cells.
Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating
Authors: Litvinova Larisa , Yurova Kristina , Shupletsova Valeria , Khaziakhmatova Olga , Malashchenko Vladimir , Shunkin Egor , Melashchenko Elena , Todosenko Natalia , Khlusova Marina , Sharkeev Yurii , Komarova Ekaterina , Sedelnikova Maria , Khlusov Igor
Published: October 2020

The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (RUNX2, BMP2, BMP6, BGLAP, and ALPL) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.
Digital Phenotyping in Bipolar Disorder: Which Integration with Clinical Endophenotypes and Biomarkers?
Authors: Orsolini Laura , Fiorani Michele , Volpe Umberto
Published: October 2020

Bipolar disorder (BD) is a complex neurobiological disorder characterized by a pathologic mood swing. Digital phenotyping, defined as the ‘moment-by-moment quantification of the individual-level human phenotype in its own environment’, represents a new approach aimed at measuring the human behavior and may theoretically enhance clinicians’ capability in early identification, diagnosis, and management of any mental health conditions, including BD. Moreover, a digital phenotyping approach may easily introduce and allow clinicians to perform a more personalized and patient-tailored diagnostic and therapeutic approach, in line with the framework of precision psychiatry. The aim of the present paper is to investigate the role of digital phenotyping in BD. Despite scarce literature published so far, extremely heterogeneous methodological strategies, and limitations, digital phenotyping may represent a grounding research and clinical field in BD, by owning the potentialities to quickly identify, diagnose, longitudinally monitor, and evaluating clinical response and remission to psychotropic drugs. Finally, digital phenotyping might potentially constitute a possible predictive marker for mood disorders.
Tiny Actors in the Big Cellular World: Extracellular Vesicles Playing Critical Roles in Cancer
Authors: Jurj Ancuta , Pop-Bica Cecilia , Slaby Ondrej , Ştefan Cristina , Cho William C. , Korban Schuyler , Berindan-Neagoe Ioana
Published: October 2020

Communications among cells can be achieved either via direct interactions or via secretion of soluble factors. The emergence of extracellular vesicles (EVs) as entities that play key roles in cell-to-cell communication offer opportunities in exploring their features for use in therapeutics; i.e., management and treatment of various pathologies, such as those used for cancer. The potential use of EVs as therapeutic agents is attributed not only for their cell membrane-bound components, but also for their cargos, mostly bioactive molecules, wherein the former regulate interactions with a recipient cell while the latter trigger cellular functions/molecular mechanisms of a recipient cell. In this article, we highlight the involvement of EVs in hallmarks of a cancer cell, particularly focusing on those molecular processes that are influenced by EV cargos. Moreover, we explored the roles of RNA species and proteins carried by EVs in eliciting drug resistance phenotypes. Interestingly, engineered EVs have been investigated and proposed as therapeutic agents in various in vivo and in vitro studies, as well as in several clinical trials.
Doxorubicin-Induced Translocation of mtDNA into the Nuclear Genome of Human Lymphocytes Detected Using a Molecular-Cytogenetic Approach
Authors: Harutyunyan Tigran , Al-Rikabi Ahmed , Sargsyan Anzhela , Hovhannisyan Galina , Aroutiounian Rouben , Liehr Thomas
Published: October 2020

Translocation of mtDNA in the nuclear genome is an ongoing process that contributes to the development of pathological conditions in humans. However, the causal factors of this biological phenomenon in human cells are poorly studied. Here we analyzed mtDNA insertions in the nuclear genome of human lymphocytes after in vitro treatment with doxorubicin (DOX) using a fluorescence in situ hybridization (FISH) technique. The number of mtDNA insertions positively correlated with the number of DOX-induced micronuclei, suggesting that DOX-induced chromosome breaks contribute to insertion events. Analysis of the odds ratios (OR) revealed that DOX at concentrations of 0.025 and 0.035 µg/mL significantly increases the rate of mtDNA insertions (OR: 3.53 (95% CI: 1.42–8.76, p < 0.05) and 3.02 (95% CI: 1.19–7.62, p < 0.05), respectively). Analysis of the distribution of mtDNA insertions in the genome revealed that DOX-induced mtDNA insertions are more frequent in larger chromosomes, which are more prone to the damaging action of DOX. Overall, our data suggest that DOX-induced chromosome damage can be a causal factor for insertions of mtDNA in the nuclear genome of human lymphocytes. It can be assumed that the impact of a large number of external and internal mutagenic factors contributes significantly to the origin and amount of mtDNA in nuclear genomes.
The Effects of Facial Expressions on Face Biometric System’s Reliability
Authors: Alrubaish Hind A. , Zagrouba Rachid
Published: October 2020

The human mood has a temporary effect on the face shape due to the movement of its muscles. Happiness, sadness, fear, anger, and other emotional conditions may affect the face biometric system’s reliability. Most of the current studies on facial expressions are concerned about the accuracy of classifying the subjects based on their expressions. This study investigated the effect of facial expressions on the reliability of a face biometric system to find out which facial expression puts the biometric system at greater risk. Moreover, it identified a set of facial features that have the lowest facial deformation caused by facial expressions to be generalized during the recognition process, regardless of which facial expression is presented. In order to achieve the goal of this study, an analysis of 22 facial features between the normal face and six universal facial expressions is obtained. The results show that the face biometric systems are affected by facial expressions where the disgust expression achieved the most dissimilar score, while the sad expression achieved the lowest dissimilar score. Additionally, the study identified the five and top ten facial features that have the lowest facial deformations on the face shape in all facial expressions. Besides that, the relativity score showed less variances between the sample using the top facial features. The obtained results of this study minimized the false rejection rate in the face biometric system and subsequently the ability to raise the system’s acceptance threshold to maximize the intrusion detection rate without affecting the user convenience.
A Survey of the Brazilian Dicranocentrus Schött (Collembola, Orchesellidae, Heteromurini) with the Description of a New Species and Notes on the Genus
Authors: Bellini Bruno C. , Cipola Nikolas G. , Siqueira Orquianne J. R.
Published: October 2020

Dicranocentrus Schött is the most diverse and widespread taxon of Neotropical Orchesellidae. In Brazil, the genus is represented by 11 species found in humid forested areas of Atlantic and Amazon forests domains. Here we describe in detail Dicranocentrus abestado sp. nov. from Chapada Diamantina, Caatinga domain, Brazil. The new species belongs to the marias group sensu Mari-Mutt, due to the absence of most posterior macrochaetae on the dorsal head, and resembles other Neotropical species with 3, 2 and 2 central macrochaetae on abdominal segments I–III. However, the new species is unique especially by its reduced colour pattern combined with its empodial complex morphology. We compare Dicranocentrus abestado sp. nov. with 27 other taxa from the New and Old World, including all species with 3 macrochaetae on the first abdominal segment; provide notes and details on the morphology of the compared species plus identification keys to Brazilian and all species of the genus with similar abdominal chaetotaxy. We also discuss the current taxonomical knowledge on Brazilian Dicranocentrus and provide notes on its chaetotaxy and Heteromurinae systematics.