bluuebunny/crossref_metadata_2025 huggingface dataset:
Parameterization of Mangrove Root Structure of Rhizophora stylosa in Coastal Hydrodynamic Model
Authors: Mori Nobuhito , Chang Che-Wei , Inoue Tomomi , Akaji Yasuaki , Hinokidani Ko , Baba Shigeyuki , Takagi Masashi , Mori Sotaro , Koike Hironoshin , Miyauchi Miho , Suganuma Ryosuke , Sabunas Audrius , Miyashita Takuya , Shimura Tomoya
Published: January 2022

Mangroves are able to attenuate tsunamis, storm surges, and waves. Their protective function against wave disasters is gaining increasing attention as a typical example of the green infrastructure/Eco-DRR (Ecosystem-based Disaster Risk Reduction) in coastal regions. Hydrodynamic models commonly employed additional friction or a drag forcing term to represent mangrove-induced energy dissipation for simplicity. The well-known Morison-type formula (Morison et al. 1950) has been considered appropriate to model vegetation-induced resistance in which the information of the geometric properties of mangroves, including the root system, is needed. However, idealized vegetation configurations mainly were applied in the existing numerical models, and only a few field observations provided the empirical parameterization of the complex mangrove root structures. In this study, we conducted field surveys on the Iriomote Island of Okinawa, Japan, and Tarawa, Kiribati. We measured the representative parameters for the geometric properties of mangroves, Rhizophora stylosa, and their root system. By analyzing the data, significant correlations for hydrodynamic modeling were found among the key parameters such as the trunk diameter at breast height (DBH), the tree height H, the height of prop roots, and the projected areas of the root system. We also discussed the correlation of these representative factors with the tree age. These empirical relationships are summarized for numerical modeling at the end.
HSP90 Inhibitor 17-AAG Attenuates Nucleus Pulposus Inflammation and Catabolism Induced by M1-Polarized Macrophages
Authors: Zhang Shuo , Wang Peng , Hu Binwu , Liu Weijian , Lv Xiao , Chen Songfeng , Shao Zengwu
Published: January 2022

Overactivated inflammation and catabolism induced by proinflammatory macrophages are involved in the pathological processes of intervertebral disc (IVD) degeneration (IVDD). Our previous study suggested the protective role of inhibiting heat shock protein 90 (HSP90) in IVDD, while the underlying mechanisms need advanced research. The current study investigated the effects of HSP90 inhibitor 17-AAG on nucleus pulposus (NP) inflammation and catabolism induced by M1-polarized macrophages. Immunohistochemical staining of degenerated human IVD samples showed massive infiltration of macrophages, especially M1 phenotype, as well as elevated levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and matrix metalloproteinase (MMP)13. The conditioned medium (CM) of inflamed NP cells (NPCs) enhanced M1 polarization of macrophages, while the CM of M1 macrophages but not M2 macrophages promoted the expression of inflammatory factors and matrix proteases in NPCs. Additionally, we found that 17-AAG could represent anti-inflammatory and anti-catabolic effects by modulating both macrophages and NPCs. On the one hand, 17-AAG attenuated the pro-inflammatory activity of M1 macrophages via inhibiting nuclear factor-κB (NF-κB) pathway and mitogen-activated protein kinase (MAPK) pathways. On the other hand, 17-AAG dampened M1-CM-induced inflammation and catabolism in NPCs by upregulating HSP70 and suppressing the Janus kinase 2 (JAK2)-signal transducer and activator of transcription 3 (STAT3) pathway. Moreover, both in vitro IVD culture models and murine disc puncture models supported that 17-AAG treatment decreased the levels of inflammatory factors and matrix proteases in IVD tissues. In conclusion, HSP90 inhibitor 17-AAG attenuates NP inflammation and catabolism induced by M1 macrophages, suggesting 17-AAG as a promising candidate for IVDD treatment.
Impacts of Climate Change and Land Use/Cover Change on Regional Hydrological Processes: Case of the Guangdong-Hong Kong-Macao Greater Bay Area
Authors: Tan Xueling , Liu Suning , Tian Yong , Zhou Zhaoqiang , Wang Yao , Jiang Jiping , Shi Haiyun
Published: January 2022

Climate change and land use/cover change (LUCC) have been widely recognized as the main driving forces that can affect regional hydrological processes, and quantitative assessment of their impacts is of great importance for the sustainable development of regional ecosystems, land use planning and water resources management. This study investigates the impacts of climate change and LUCC on variables such as streamflow (SF), soil moisture (SM) and evapotranspiration (ET) in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA) by using Soil and Water Assessment Tools (SWAT) model under different scenarios during 1979–2018. The results show that the simulation performances were overall good, with Nash-Sutcliffe Efficiency Coefficient (NSE) and coefficient of determination (R2) greater than 0.80 for the monthly-scale SF calibration and validation. According to the results of trend and change point tests of meteorological series, the baseline period (1979–1997) and the interference period (1998–2018) were determined. Interestingly, other land use types were basically converted to urban land, leading to a rapid urbanization in the GBA. Compared with the SF values of the eight estuaries of the Pearl River Basin in the baseline period, both climate change and LUCC has led to the decrease in the SF values in the interference period, and the combined effect of climate change and LUCC was slightly greater than their individual effect. Overall, climate change and LUCC both have important impacts on regional hydrological processes in the GBA.
Precautionary Principle or Evidence-Based Conservation? Assessing the Information Content of Threat Data for the Yangtze Finless Porpoise
Authors: Mogensen Lisa M. W. , Mei Zhigang , Hao Yujiang , Harrison Xavier A. , Wang Ding , Turvey Samuel T.
Published: January 2022

Conservation management requires evidence, but robust data on key parameters such as threats are often unavailable. Conservation-relevant insights might be available within datasets collected for other reasons, making it important to determine the information content of available data for threatened species and identify remaining data-gaps before investing time and resources in novel data collection. The Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis) has declined severely across the middle-lower Yangtze, but multiple threats exist in this system and the relative impact of different anthropogenic activities is unclear, preventing identification of appropriate mitigation strategies. Several datasets containing information on porpoises or potential threats are available from past boat-based and fishing community surveys, which might provide novel insights into causes of porpoise mortality and decline. We employed multiple analytical approaches to investigate spatial relationships between live and dead porpoises and different threats, reproductive trends over time, and sustainable offtake levels, to assess whether evidence-based conservation is feasible under current data availability. Our combined analyses provide new evidence that mortality is spatially associated with increased cargo traffic; observed mortality levels (probably a substantial underestimate of true levels) are unsustainable; and population recruitment is decreasing, although multiple factors could be responsible (pollutants, declining fish stocks, anthropogenic noise, reduced genetic diversity). Available data show little correlation between patterns of mortality and fishing activity even when analyzed across multiple spatial scales; however, interview data can be affected by multiple biases that potentially complicate attempts to reconstruct levels of bycatch, and new data are required to understand dynamics and sustainability of porpoise-fisheries interactions. This critical assessment of existing data thus suggests thatin situporpoise conservation management must target multiple co-occurring threats. Even limited available datasets can provide new insights for understanding declines, and we demonstrate the importance of an integrative approach for investigating complex conservation problems and maximizing evidence in conservation planning for poorly known taxa.
The Role of NF-kB in the Downregulation of Organic Cation Transporter 2 Expression and Renal Cation Secretion in Kidney Disease
Authors: Han Chao , Zheng Juan , Wang Fengyi , Lu Qingyang , Chen Qingfa , Hu Ankang , Visentin Michele , Kullak-Ublick Gerd A. , Gai Zhibo , Chu Lei
Published: January 2022

Organic cation transporter 2 (OCT2), encoded by the SLC22A2 gene, is the main cation transporter on the basolateral membrane of proximal tubular cells. OCT2 facilitates the entry step of the vectorial transport of most cations from the peritubular space into the urine. OCT2 downregulation in kidney disease models is apparent, yet not clear from a mechanistic vantage point. The aim of this study was to explore the role of inflammation, a common thread in kidney disease, and NF-kB in OCT2 modulation and tubular secretion. Among the OCTs, OCT2 was found consistently downregulated in the kidney of rats with chronic kidney disease (CKD) or acute kidney injury (AKI) and in patients diagnosed with CKD, and it was associated with the upregulation of TNFα renal expression. Exposure to TNFα reduced the expression and function of OCT2 in primary renal proximal tubule epithelial cells (RPTEC). Silencing or pharmacological inhibition of NF-kB rescued the expression of OCT2 in the presence of TNFα, indicating that OCT2 repression was NF-kB-dependent. In silico prediction coupled to gene reporter assay demonstrated the presence of at least one functional NF-kB cis-element upstream the transcription starting site of the SLC22A2 gene. Acute inflammation triggered by lipopolysaccharide injection induced TNFα expression and the downregulation of OCT2 in rat kidney. The inflammation did reduce the active secretion of the cation Rhodamine 123, with no impairment of the glomerular filtration. In conclusion, the NF-kB pathway plays a major role in the transcriptional regulation of OCT2 and, in turn, in the overall renal secretory capacity.
Phenolic-Rich Plant Extracts With Antimicrobial Activity: An Alternative to Food Preservatives and Biocides?
Authors: Oulahal Nadia , Degraeve Pascal
Published: January 2022

In recent years, the search for natural plant-based antimicrobial compounds as alternatives to some synthetic food preservatives or biocides has been stimulated by sanitary, environmental, regulatory, and marketing concerns. In this context, besides their established antioxidant activity, the antimicrobial activity of many plant phenolics deserved increased attention. Indeed, industries processing agricultural plants generate considerable quantities of phenolic-rich products and by-products, which could be valuable natural sources of natural antimicrobial molecules. Plant extracts containing volatile (e.g., essential oils) and non-volatile antimicrobial molecules can be distinguished. Plant essential oils are outside the scope of this review. This review will thus provide an overview of current knowledge regarding the promises and the limits of phenolic-rich plant extracts for food preservation and biofilm control on food-contacting surfaces. After a presentation of the major groups of antimicrobial plant phenolics, of their antimicrobial activity spectrum, and of the diversity of their mechanisms of action, their most promising sources will be reviewed. Since antimicrobial activity reduction often observed when comparing in vitro and in situ activities of plant phenolics has often been reported as a limit for their application, the effects of the composition and the microstructure of the matrices in which unwanted microorganisms are present (e.g., food and/or microbial biofilms) on their activity will be discussed. Then, the different strategies of delivery of antimicrobial phenolics to promote their activity in such matrices, such as their encapsulation or their association with edible coatings or food packaging materials are presented. The possibilities offered by encapsulation or association with polymers of packaging materials or coatings to increase the stability and ease of use of plant phenolics before their application, as well as to get systems for their controlled release are presented and discussed. Finally, the necessity to consider phenolic-rich antimicrobial plant extracts in combination with other factors consistently with hurdle technology principles will be discussed. For instance, several authors recently suggested that natural phenolic-rich extracts could not only extend the shelf-life of foods by controlling bacterial contamination, but could also coexist with probiotic lactic acid bacteria in food systems to provide enhanced health benefits to human.
BTBD10 is a Prognostic Biomarker Correlated With Immune Infiltration in Hepatocellular Carcinoma
Authors: Li Jianhui , Tian Xiaojuan , Nie Ye , He Ying , Wu Wenlong , Lei Xinjun , Zhang Tianchen , Wang Yanfang , Mao Zhenzhen , Zhang Hong , Zhang Xuan , Song Wenjie
Published: January 2022

Background: BTBD10 serves as an activator of Akt family members through decreasing the protein phosphatase 2A-mediated dephosphorylation. The present study attempted to investigate the prognostic value of BTBD10 in hepatocellular carcinoma (HCC), specially, its relationship with tumor-infiltrating lymphocytes (TILs).

Methods: BTBD10 expression was evaluated in HCC using The Cancer Genome Atlas (TCGA) and Xijing Hospital database, and verified in HCC cell lines. Cox analyses were performed to analyze independent prognostic risk factors for HCC. The optimal cut-off value of BTBD10 was calculated, by which all patients were divided into two groups to compare the overall survival (OS). The signaling pathways were predicted, by which BTBD10 may affect the progression of HCC. To investigate the impact of BTBD10 on HCC immunotherapy, correlations between BTBD10 and TILs, immune checkpoints, m6A methylation-related genes and ferroptosis-related genes were assessed. The distribution of half-maximal inhibitory concentration (IC50) of diverse targeted drugs was observed based on the differential expression of BTBD10.

Results: BTBD10 expression was higher in HCC tissues and cell lines than that of normal liver tissues and cells. The patients with high expression of BTBD10 showed a worse OS, as compared to that of BTBD10 low-expressing group. Cox analyses indicated that BTBD10 was an independent prognostic risk factor for HCC. Several molecular pathways of immune responses were activated in HCC patients with high-expressing of BTBD10. Furthermore, BTBD10 expression was demonstrated to be positively correlated with tumor-infiltrating B cells, T cells, macrophages, neutrophils and dendritic cells. Meanwhile, the expression of BTBD10 was synchronized with that of several m6A methylation-related genes, ferroptosis-related genes and immune checkpoints. The IC50 scores of Sorafenib, Navitoclax, Veliparib, Luminespib, and Imatinib were found to be lower in BTBD10 high-expressing HCC group.

Conclusion: BTBD10 negatively regulates tumor immunity in HCC and exhibits adverse effect on the prognosis of HCC, which could be a potential target for immunotherapy.
Skin Color and Attractiveness Modulate Empathy for Pain: An Event-Related Potential Study
Authors: Yang Di , Li Xiong , Zhang Yinya , Li Zuoshan , Meng Jing
Published: January 2022

Although racial in-group bias in empathy for pain has been reported, empathic responses to others’ pain may be influenced by other characteristics besides race. To explore whether skin color and attractiveness modulate empathy for pain, we recorded 24 participants’ reactions to painful faces from racial in-group members with different skin color (fair, wheatish, or dark) and attractiveness (more or less attractive) using event-related potentials (ERPs). Results showed that, for more attractive painful faces, dark skin faces were judged as less painful and elicited smaller N2 amplitudes than fair- and wheatish-skinned faces. However, for less attractive faces, there were no significant differences among the three skin colors. Our findings suggest that empathy for pain toward racial in-group members may be influenced by skin color and attractiveness.
COVID-19's Impact on China's Strategic Emerging Industries: An Observation of Policy Difficulties
Authors: Li Dexuan , Dai Wensheng , Guan Weimin
Published: January 2022

The study investigates the influence of the COVID-19 on the rate of R&D investment and foreign exchange development of China's most important emerging industry firms. From 2010 to 2020, data were collected from 26 locations across China, focusing on seven different types of critical creating companies. To analyze the data, we have applied Fourier Increased Unit Root Test, Granger causality assessments test, Pattern Assessment test, Poisson pseudo most excellent probability (PPML) approach, Wald test, and Regression analysis test. The results of the tests reveal a clear underlying association among COVID-19 relates Chinese exports and imports. COVID-19's instant effects on imports and exports lack working capital have been calculated, but the short-term, medium-to-long-term products are composite and unidentified. The article result main results are following: (i) The COVID-19 impacts the R&D investment is main industries like as high-end equipment industry, new materials industry, and new-era data innovation. (ii) The COVID-19 highly affects the imports and exports development network of Chinese strategic emerging industries which emphasizes cross-industry grouping features. The study provides the guidance to the future researchers to focus on COVID-19 affects on the strategic emerging industries of developed and underdeveloped countries to determine of foreign direct investment inflow and unemployment growth rates.

JEL: G20, O10, O40
Intestinal Protists in Captive Non-human Primates and Their Handlers in Six European Zoological Gardens. Molecular Evidence of Zoonotic Transmission
Authors: Köster Pamela C. , Martínez-Nevado Eva , González Andrea , Abelló-Poveda María T. , Fernández-Bellon Hugo , de la Riva-Fraga Manuel , Marquet Bertille , Guéry Jean-Pascal , Knauf-Witzens Tobias , Weigold Annika , Dashti Alejandro , Bailo Begoña , Imaña Elena , Muadica Aly S. , González-Barrio David , Ponce-Gordo Francisco , Calero-Bernal Rafael , Carmena David
Published: January 2022

We assessed the occurrence, genetic diversity, and zoonotic potential of four protozoan (Cryptosporidium spp., Entamoeba histolytica, Entamoeba dispar, Giardia duodenalis), one stramenopile (Blastocystis sp.), one microsporidia (Enterocytozoon bieneusi), and two ciliate (Balantioides coli, Troglodytella abrassarti) intestinal parasite or commensal protist species in captive non-human primates (NHP) and their zookeepers from six European zoological gardens in France (n = 1), Germany (n = 1), and Spain (n = 4). Faecal samples from NHP (n = 454) belonging to 63 species within 35 genera and humans (n = 70) were collected at two sampling periods in each participating institution between October 2018-August 2021. Detection and species identification was accomplished by PCR and Sanger sequencing of the ssu rRNA and/or ITS genes. Sub-genotyping analyses using specific markers were conducted on isolates positive for G. duodenalis (gdh, bg, tpi) and Cryptosporidium spp. (gp60). Overall, 41.0% (186/454) and 30.0% (21/70) of the faecal samples of NHP and human origin tested positive for at least one intestinal protist species, respectively. In NHP, Blastocystis sp. was the most prevalent protist species found (20.3%), followed by G. duodenalis (18.1%), E. dispar (7.9%), B. coli and T. abrassarti (1.5% each), and Cryptosporidium spp. and E. bieneusi (0.9% each). Occurrence rates varied largely among NHP host species, sampling periods, and zoological institutions. The predominant protist species found in humans was Blastocystis sp. (25.7%), followed by Cryptosporidium spp. (2.9%), E. dispar (1.4%), and G. duodenalis (1.4%). Sequencing of PCR-positive amplicons in human and/or NHP confirmed the presence of Cryptosporidium in six isolates (C. hominis: 66.7%, C. parvum: 33.3%), G. duodenalis in 18 isolates (assemblage A: 16.7%, assemblage B: 83.3%), Blastocystis in 110 isolates (ST1:38.2%, ST2:11.8%, ST3: 18.2%, ST4: 9.1%, ST5: 17.3%, ST8: 2.7%, ST13: 0.9%), and E. bieneusi in four isolates (CM18: 75.0%, Type IV: 25.0%). Zoonotic transmission events involving Blastocystis ST1–ST4 were identified in four zoological institutions. Zoonotic transmission of C. hominis was highly suspected, but not fully demonstrated, in one of them. Monitoring of intestinal protist species might be useful for assessing health status of captive NHP and their zookeepers, and to identify transmission pathways of faecal-orally transmitted pathogens.
Inflammation in Focus: The Beginning and the End
Authors: L. Kiss Anna
Published: January 2022

The inflammation is an important biological response induced by various harmful stimuli, like viruses, bacterial infections, toxins, toxic compounds, tissue injury. During inflammation inflammatory cytokines and reactive oxygen species are produced. Inflammatory cytokines act on various receptors present on the plasma membrane of target cells. To initiate signaling cascade, and activate transcription factors, receptors should be internalized and enter the early endosomes, where the members of the signaling cascade can meet. The further cytoplasmic fate of the receptor plays crucial role in the progression and the course of inflammation. Usually acute inflammation removes injurious stimuli and helps to regain the normal healthy status of the organism. In contrast to this the uncontrolled chronic inflammation—stimulating other than immune cells, inducing transdifferentiation—can provide base of various serious diseases. This paper draws the attention of the long-lasting consequence of chronic inflammation, pointing out that one of the most important step in medication is to identify in time the factors initiating and maintaining inflammation.
Impact-Angle and Terminal-Maneuvering-Acceleration Constrained Guidance against Maneuvering Target
Authors: Zhang Wanqing , Chen Wanchun , Yu Wenbin
Published: January 2022

A new, highly constrained guidance law is proposed against a maneuvering target while satisfying both impact angle and terminal acceleration constraints. Here, the impact angle constraint is addressed by solving an optimal guidance problem in which the target’s maneuvering acceleration is time-varying. To deal with the terminal acceleration constraint, the closed-form solutions of the new guidance are needed. Thus, a novel engagement system based on the guidance considering the target maneuvers is put forward by choosing two angles associated with the relative velocity vector and line of sight (LOS) as the state variables, and then the system is linearized using small angle assumptions, which yields a special linear time-varying (LTV) system that can be solved analytically by the spectral-decomposition-based method. For the general case where the closing speed, which is the speed of approach of the missile and target, is allowed to change with time arbitrarily, the solutions obtained are semi-analytical. In particular, when the closing speed changes linearly with time, the completely closed-form solutions are derived successfully. By analyzing the generalized solutions, the stability domain of the guidance coefficients is obtained, in which the maneuvering acceleration of the missile can converge to zero finally. Here, the key to investigating the stability domain is to find the limits of some complicated integral terms of the generalized solutions by skillfully using the squeeze theorem. The advantages of the proposed guidance are demonstrated by conducting trajectory simulations.
The Effects of Foliar Application of Phenoxy and Imidazoline Family Herbicides on the Limitation of Primary Photosynthetic Processes in Galega orientalis L.
Authors: Lang Jaroslav , Zikmundová Barbora , Hájek Josef , Barták Miloš , Váczi Peter
Published: January 2022

Fodder galega (Galega orientalis) is a perennial, wintering plant with great potential for agricultural development. The species has a large yield potential and exceptional adaptability to various environmental conditions. The sensitivity of G. orientalis to herbicides, however, as well as the photosynthetic performance of the species, are generally unknown. Our study aimed to evaluate the effects of the application of selected phenoxy herbicides (MCPA, MCPB) and the imidazoline family herbicide (IMA) on the parameters of primary photosynthetic processes as understood through fast chlorophyll fluorescence kinetics (OJIP). The effect of cultivation temperature was also investigated in the plants grown at 5, 18 and 25 °C. Time courses of OJIP-derived parameters describing photosystem II functioning after foliar application revealed that the plants showed negative responses to the herbicides in the order MCPB–MCPA–IMA within 24 h after the application. The application of herbicides decreased the values of maximum chlorophyll fluorescence (FM) and increased minimum fluorescence (F0), which led to a reduction in the maximal efficiency of PSII (FV/FM). Applications of MCPA and MCPB decreased variable chlorophyll fluorescence at 2 ms (VJ), 30 ms (VI) and VP, as well as the performance index (PIABS), which is considered a vitality proxy. The application increased absorption flux (ABS/RC), trapped energy flux (TRo/RC) and dissipated energy flux (DIo/RC). The effects were more pronounced in plants grown at 18 and 25 °C. The study revealed that the OJIP-derived parameters sensitively reflected an early response of G. orientalis to the foliar application of herbicides. Negative responses of PSII were more apparent in MCPA- and MCPB- exposed plants than IMA-exposed plants.
A Systematic Review and Meta-Analysis of the Prevalence and Impact of Pulmonary Bacterial Colonisation in Stable State Chronic Obstructive Pulmonary Disease (COPD)
Authors: Armitage Michael N. , Spittle Daniella A. , Turner Alice M.
Published: January 2022

Background: Half of acute exacerbations of COPD are due to bacterial infection, and the other half are likely influenced by microbial colonisation. The same organisms commonly cultured during acute exacerbations are often found in the sputum of patients during stability. A robust assessment of the prevalence of potentially pathogenic microorganisms (PPMs) in the sputum of stable COPD patients may help to inform the targeted prevention of exacerbation by these organisms. Methods: A systematic review and meta-analysis was carried out to determine the prevalence of PPMs in patients with COPD in the stable state. Meta-analysis of prevalence was carried out using the Freeman–Tukey double arcsine transformation random effects model, and sub-group analysis was performed for sputum modality. Prevalence of total and individual PPMs was calculated from patient-level data from individual studies. Results: Pooled prevalence of PPMs identified by sputum culture was found to be 41% (95% CI 36–47%). Significant heterogeneity was found across all studies, which can likely be attributed to inconsistent measuring and reporting of PPMs. The most commonly reported organisms were H. influenzae, M catarrhalis, S. pneumoniae, S. aureus, and P. aeruginosa. Declining lung function was weakly correlated with prevalence of PPMs. Conclusion: The airways of patients with COPD are colonised with PPMs during the stable state in almost half of patients. A complex relationship likely exists between the microbiome in the stable state and the phenotype of COPD patients. Targeted microbial therapy for preventing exacerbations of COPD should carefully consider the stable microbiome as well as the exacerbated.
DNA Damage Repair: Predictor of Platinum Efficacy in Ovarian Cancer?
Authors: Stefanou Dimitra T. , Souliotis Vassilis L. , Zakopoulou Roubini , Liontos Michalis , Bamias Aristotelis
Published: January 2022

Ovarian cancer (OC) is the seventh most common type of cancer in women worldwide. Treatment for OC usually involves a combination of surgery and chemotherapy with carboplatin and paclitaxel. Platinum-based agents exert their cytotoxic action through development of DNA damage, including the formation of intra- and inter-strand cross-links, as well as single-nucleotide damage of guanine. Although these agents are highly efficient, intrinsic and acquired resistance during treatment are relatively common and remain a major challenge for platinum-based therapy. There is strong evidence to show that the functionality of various DNA repair pathways significantly impacts tumor response to treatment. Various DNA repair molecular components were found deregulated in ovarian cancer, including molecules involved in homologous recombination repair (HRR), nucleotide excision repair (NER), mismatch repair (MMR), non-homologous end-joining (NHEJ), and base excision repair (BER), which can be possibly exploited as novel therapeutic targets and sensitive/effective biomarkers. This review attempts to summarize published data on this subject and thus help in the design of new mechanistic studies to better understand the involvement of the DNA repair in the platinum drugs resistance, as well as to suggest new therapeutic perspectives and potential targets.
Stable Gastric Pentadecapeptide BPC 157 Therapy of Rat Glaucoma
Authors: Kralj Tamara , Kokot Antonio , Zlatar Mirna , Masnec Sanja , Kasnik Kovac Katarina , Milkovic Perisa Marija , Batelja Vuletic Lovorka , Giljanovic Ana , Strbe Sanja , Sikiric Suncana , Balog Slaven , Sontacchi Bojan , Sontacchi Dijana , Buljan Matko , Lovric Eva , Boban Blagaic Alenka , Skrtic Anita , Seiwerth Sven , Sikiric Predrag
Published: January 2022

Cauterization of three episcleral veins (open-angle glaucoma model) induces venous congestion and increases intraocular pressure in rats. If not upgraded, one episcleral vein is regularly unable to acquire and take over the whole function, and glaucoma-like features persist. Recently, the rapid upgrading of the collateral pathways by a stable gastric pentadecapeptide BPC 157 has cured many severe syndromes induced by permanent occlusion of major vessels, veins and/or arteries, peripherally and centrally. In a six-week study, medication was given prophylactically (immediately before glaucoma surgery, i.e., three episcleral veins cauterization) or as curative treatment (starting at 24 h after glaucoma surgery). The daily regimen of BPC 157 (0.4 µg/eye, 0.4 ng/eye; 10 µg/kg, 10 ng/kg) was administered locally as drops in each eye, intraperitoneally (last application at 24 h before sacrifice) or per-orally in drinking water (0.16 µg/mL, 0.16 ng/mL, 12 mL/rat until the sacrifice, first application being intragastric). Consequently, all BPC 157 regimens immediately normalized intraocular pressure. BPC 157-treated rats exhibited normal pupil diameter, microscopically well-preserved ganglion cells and optic nerve presentation, normal fundus presentation, normal retinal and choroidal blood vessel presentation and normal optic nerve presentation. As leading symptoms, increased intraocular pressure and mydriasis, as well as degeneration of retinal ganglion cells, optic nerve head excavation and reduction in optic nerve thickness, generalized severe irregularity of retinal vessels, faint presentation of choroidal vessels and severe optic nerve disc atrophy were all counteracted. In conclusion, we claim that the reversal of the episcleral veins cauterization glaucoma appeared as a consequence of the BPC 157 therapy of the vessel occlusion-induced perilous syndrome.
Impact of the Covering Vegetable Oil on the Sensory Profile of Canned Tuna of Katsuwonus pelamis Species and Tuna’s Taste Evaluation Using an Electronic Tongue
Authors: Ferreiro Nuno , Rodrigues Nuno , Veloso Ana C. A. , Fernandes Conceição , Paiva Helga , Pereira José A. , Peres António M.
Published: January 2022

The impact of the covering vegetable oil (sunflower oil, refined olive oil and extra virgin olive oil, EVOO) on the physicochemical and sensory profiles of canned tuna (Katsuwonus pelamis species) was evaluated, using analytical techniques and a sensory panel. The results showed that canned tuna covered with EVOO possesses a higher content of total phenols and an enhanced antioxidant capacity. This covering medium also increased the appreciated redness-yellowness color of the canned tuna, which showed a higher chromatic and intense color. Olfactory and kinesthetic sensations were significantly dependent on the type of oil used as covering medium. Tuna succulence and adhesiveness were promoted by the use of EVOO, which also contributed to decreasing the tuna-related aroma sensations. The tuna sensory data could be successfully used to identify the type of vegetable oil used. Moreover, a potentiometric electronic tongue allowed discriminating between the canned tuna samples according to the vegetable oil used (mean sensitivity of 96 ± 8%; repeated K-fold cross-validation) and the fruity intensity of the EVOO (mean sensitivity of 100%; repeated K-fold cross-validation). Thus, the taste sensor device could be a practical tool to verify the authenticity of the declared covering medium in canned tuna and to perceive the differences in consumers’ taste.
Frequency and Determinants of Breastfeeding in Greece: A Prospective Cohort Study during the COVID-19 Pandemic
Authors: Tigka Maria , Metallinou Dimitra , Nanou Christina , Iliodromiti Zoi , Lykeridou Katerina
Published: January 2022

Breastfeeding is considered to be the cornerstone of child health. In Europe however, overall breastfeeding rates remain low. The present study aimed at estimating the frequency of breastfeeding in Greece during the COVID-19 pandemic period and comparing findings with the latest national study in order to identify a potential impact of the pandemic. Additionally, possible correlations of socio-cultural and demographic characteristics with breastfeeding indicators were investigated. This prospective cohort study included 847 women from five tertiary maternity hospitals, between January and December 2020. Data were collected by a structured questionnaire via interview during hospitalization and via telephone in the first, third and sixth month postpartum. Results showed that all breastfeeding indicators improved over the last three years. Full breastfeeding reached 7.2%, contrary to 0.78% of the latest national study at six months postpartum. Employment, marital status, educational level, mode of delivery, type of maternity hospital, body mass index before pregnancy, previous breastfeeding experience of the mother and infant’s birth weight correlated significantly with breastfeeding indicators at different time periods. The COVID-19 pandemic seems to have favorably influenced breastfeeding initiation and duration in Greece due to lockdowns, home confinement and teleworking.
Role of Spanish Toddlers’ Education and Care Institutions in Achieving Physical Activity Recommendations in the COVID-19 Era: A Cross-Sectional Study
Authors: Vega-Perona Herminia , Estevan Isaac , García-Ochoa Yolanda Cabrera , Martínez-Bello Daniel A. , Bernabé-Villodre María del Mar , Martínez-Bello Vladimir E.
Published: January 2022

To our knowledge, there are no published studies that describe the physical activity (PA) levels and objectively measure them through accelerometry in toddlers (2–3 years old) attending early childhood education and care (ECEC) institutions during the COVID-19 pandemic. The aims of this study were two-fold: (a) to analyse toddlers’ PA levels and sedentary behaviour (SB) during school hours in ECEC institutions, as well as the rate of adherence to specific recommendations on total PA (TPA) and moderate–vigorous PA (MVPA); and (b) to evaluate the characteristics correlates (age, gender, and body mass index –BMI) of young children and the school environment on toddlers’ TPA, light PA (LPA), MVPA, and SB during school hours in ECEC institutions. PA was evaluated with ActiGraph accelerometers. The main findings were that: (a) toddlers engaged in very high amounts of TPA and MVPA during ECEC hours; (b) girls and boys displayed similar levels of LPA, TPA, and SB, while girls had lower levels of MVPA, compared to boys, and younger toddlers were less active than older ones; (c) BMI was not associated with PA of any intensity or SB; (d) playground and classroom density were not associated with higher levels of PA of any intensity, though classroom density was associated with SB. These ECEC institutions provide and challenge the new COVID-19 scenario, as well as supportive environments for toddlers’ PA.
Hexagon Fraud: Detection of Fraudulent Financial Reporting in State-Owned Enterprises Indonesia
Authors: Achmad Tarmizi , Ghozali Imam , Pamungkas Imang Dapit
Published: January 2022

This study aims to detect fraudulent financial reporting using hexagon fraud analysis, including seven factors: financial stability, external pressures, ineffective monitoring, auditor changes, change in director, arrogance, and collusion. The subject of this research is a public company consolidated audit report of state-owned enterprises. The existence of conflicting results, the phenomenon of fraudulent financial reporting, and limited research using the hexagon of fraud theory prompted this research to examine the factors that influence fraudulent financial reporting. The sample was selected using a sampling technique, with the criteria of state-owned enterprises listed on the Indonesia Stock Exchange in 2016–2020. The method used is quantitative, and the analytical method used is logistic regression analysis. The sampling technique used was purposeful sampling, so the number of samples was 125. The results of this study indicate that financial stability and external pressures have a positive effect on fraudulent financial reporting. However, ineffective monitoring, auditor changes, change in director, arrogance, and collusion do not affect fraudulent financial reporting.
Study on Air-to-Water Heat Pumps Seasonal Performances for Heating in Greece
Authors: Mouzeviris Georgios A. , Papakostas Konstantinos T.
Published: January 2022

Air-to-water heat pumps (AWHPs) is a very good option for efficient heating in the residential and commercial building sectors. Their performance and therefore the use of primary energy and CO2 gas emissions are affected by various factors. The aim of this paper is to present a study on the seasonal coefficient of performance in heating (SCOP) of AWHPs, which are available in the Greek market. The sample consists of 100 models in total, offered by 12 manufacturers, in a range of heat pump’s thermal capacity up to 50 kW. The calculation of SCOP values was performed according to the methodology proposed by the EN14825 standard. The results indicate how the heating capacity, the local climate, the supply water temperature, the compressor’s technology, and the control system affect the seasonal performance of the various AWHP models examined. Setting the SCOP ≥ 3 value as a criterion, the analysis that was carried out in four climatic zones A, B, C, and D of Greece, shows that there are many models that meet this criterion, and, in fact, their number increases from the coldest to warmer climates, in combination with lower water supply temperatures to the heating system and a control system with weather compensation.
A Multi-Point Geostatistical Seismic Inversion Method Based on Local Probability Updating of Lithofacies
Authors: Wang Zhihong , Chen Tiansheng , Hu Xun , Wang Lixin , Yin Yanshu
Published: January 2022

In order to solve the problem that elastic parameter constraints are not taken into account in local lithofacies updating in multi-point geostatistical inversion, a new multi-point geostatistical inversion method with local facies updating under seismic elastic constraints is proposed. The main improvement of the method is that the probability of multi-point facies modeling is combined with the facies probability reflected by the optimal elastic parameters retained from the previous inversion to predict and update the current lithofacies model. Constrained by the current lithofacies model, the elastic parameters were obtained via direct sampling based on the statistical relationship between the lithofacies and the elastic parameters. Forward simulation records were generated via convolution and were compared with the actual seismic records to obtain the optimal lithofacies and elastic parameters. The inversion method adopts the internal and external double cycle iteration mechanism, and the internal cycle updates and inverts the local lithofacies. The outer cycle determines whether the correlation between the entire seismic record and the actual seismic record meets the given conditions, and the cycle iterates until the given conditions are met in order to achieve seismic inversion prediction. The theoretical model of the Stanford Center for Reservoir Forecasting and the practical model of the Xinchang gas field in western China were used to test the new method. The results show that the correlation between the synthetic seismic records and the actual seismic records is the best, and the lithofacies matching degree of the inversion is the highest. The results of the conventional multi-point geostatistical inversion are the next best, and the results of the two-point geostatistical inversion are the worst. The results show that the reservoir parameters obtained using the local probability updating of lithofacies method are closer to the actual reservoir parameters. This method is worth popularizing in practical exploration and development.
Microscopic Characterization of the Mucous Cells and Their Mucin Secretions in the Alimentary Canal of the Blackmouth Catshark Galeus melastomus (Chondrichthyes: Elasmobranchii)
Authors: Bosi Giampaolo , Merella Paolo , Maynard Barbara J. , Sayyaf Dezfuli Bahram
Published: January 2022

Sharks belong to the most primitive group of jawed vertebrates and have some special structural and functional features such as a cartilaginous skeleton, a spiral intestinal valve, and a rectal gland for osmoregulation. In January 2020, ten specimens of Galeus melastomus, the Blackmouth catshark, were collected from the Gulf of Asinara (North Sardinia, Italy) and the entire alimentary canal was studied using histochemical reactions to characterize the mucous cell types. In the alimentary canal of G. melastomus, mucous cells mainly secrete a mixture of acidic and neutral mucins. Of the acidic mucins, only the carboxylated type was present in mucous cells of the stomach, while the sulfated type predominated in the esophagus and the intestines. The use of lectins revealed a distribution of sugar residues in mucins related to cellular activities of the different regions of the catshark alimentary canal. The current study is the first report to characterize the intestinal mucous cells of G. melastomus and to provide quantitative data on their different populations in the alimentary canal.
Effects of Wheel Rotation on Long-Period Wake Dynamics of the DrivAer Fastback Model
Authors: Aultman Matthew , Auza-Gutierrez Rodrigo , Disotell Kevin , Duan Lian
Published: January 2022

Lattice Boltzmann method (LBM) simulations were performed to capture the long-period dynamics within the wake of a realistic DrivAer fastback model with stationary and rotating wheels. The simulations showed that the wake developed as a low-pressure torus regardless of whether the wheels were rotating. This torus shrank in size on the base in the case of rotating wheels, leading to a reduction in the low-pressure footprint on the base, and consequently a 7% decrease in the total vehicle drag in comparison to the stationary wheels case. Furthermore, the lateral vortex shedding experienced a long-period switching associated with the bi-stability in both the stationary and rotating wheels cases. This bi-stability contributed to low-frequency side force oscillations (<1 Hz) in alignment with the peak motion-sickness-inducing frequency (0.2 Hz).
Design Considerations of Low Bypass Ratio Mixed Flow Turbofan Engines with Large Power Extraction
Authors: Rosell Daniel , Grönstedt Tomas
Published: January 2022

The possibility of extracting large amounts of electrical power from turbofan engines is becoming increasingly desirable from an aircraft perspective. The power consumption of a future fighter aircraft is expected to be much higher than today’s fighter aircraft. Previous work in this area has concentrated on the study of power extraction for high bypass ratio engines. This motivates a thorough investigation of the potential and limitations with regards to performance of a low bypass ratio mixed flow turbofan engine. A low bypass ratio mixed flow turbofan engine was modeled, and key parts of a fighter mission were simulated. The investigation shows how power extraction from the high-pressure turbine affects performance of a military engine in different parts of a mission within the flight envelope. An important conclusion from the analysis is that large amounts of power can be extracted from the turbofan engine at high power settings without causing too much penalty on thrust and specific fuel consumption, if specific operating conditions are fulfilled. If the engine is operating (i) at, or near its maximum overall pressure ratio but (ii) further away from its maximum turbine inlet temperature limit, the detrimental effect of power extraction on engine thrust and thrust specific fuel consumption will be limited. On the other hand, if the engine is already operating at its maximum turbine inlet temperature, power extraction from the high-pressure shaft will result in a considerable thrust reduction. The results presented will support the analysis and interpretation of fighter mission optimization and cycle design for future fighter engines aimed for large power extraction. The results are also important with regards to aircraft design, or more specifically, in deciding on the best energy source for power consumers of the aircraft.
Sustainable Valorization of Sambucus nigra L. Berries: From Crop Biodiversity to Nutritional Value of Juice and Pomace
Authors: Costa Carina Pedrosa , Patinha Samuel , Rudnitskaya Alisa , Santos Sónia A. O. , Silvestre Armando J. D. , Rocha Sílvia M.
Published: January 2022

Improvement of dietary and ecological biodiversity, namely by exploring autochthonous varieties, is a key point to the construction of a more sustainable food system and planetary health. However, the environmental sustainability continues to face huge challenges, reflecting the importance of achieving a better understanding about the functional role of biodiversity in ecosystems. Thus, the main objective of this research is to contribute to the sustainable valorization of Sambucus nigra L. berries through a comprehensive approach to evaluate the effects of elderberry’s cultivar, harvest year, and plantation field on the physicochemical berry composition. Moreover, the nutritional value of elderberry juice and respective dried pomace was determined. This complementary information is of huge utility for the rational and, as much as possible, integral use of elderberries. The harvest year, followed by field and the interaction of harvest × field, accounted for the highest impact on the berry’s physicochemical parameters, indicating the importance of the combined impact of the macro- and mesoclimate conditions on plant metabolism. Elderberry juice and dried pomace are a good source of carbohydrates (ca. 12 and 82%, respectively) and have low amounts of fat (≤2.5%), making them low-energy foods. Dried pomace may also represent a potential alternative source of vegetal protein (ca. 6%).