bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunophenotype of Measurable Residual Blast Cells as an Additional Prognostic Factor in Adults with B-Cell Acute Lymphoblastic Leukemia
Authors: Davydova Yulia , Galtseva Irina , Kapranov Nikolay , Nikiforova Ksenia , Aleshina Olga , Chabaeva Yulia , Isinova Galina , Kotova Ekaterina , Sokolov Andrey , Troitskaya Vera , Kulikov Sergey , Parovichnikova Elena
Published: December 2022

Measurable residual disease (MRD) is a well-known independent prognostic factor in acute leukemias, and multicolor flow cytometry (MFC) is widely used to detect MRD. MFC is able not only to enumerate MRD accurately but also to describe an antigen expression profile of residual blast cells. However, the relationship between MRD immunophenotype and patient survival probability has not yet been studied. We determined the prognostic impact of MRD immunophenotype in adults with B-cell acute lymphoblastic leukemia (B-ALL). In a multicenter study RALL-2016 (NCT03462095), 267 patients were enrolled from 2016 to 2022. MRD was assessed at the end of induction (day 70) in 94 patients with B-ALL by six- or 10-color flow cytometry in the bone marrow specimens. The 4 year relapse-free survival (RFS) was lower in MRD-positive B-ALL patients [37% vs. 78% (p < 0.0001)]. The absence of CD10, positive expression of CD38, and high expression of CD58 on MRD cells worsened the 4 year RFS [19% vs. 51% (p = 0.004), 0% vs. 51% (p < 0.0001), and 21% vs. 40% (p = 0.02), respectively]. The MRD immunophenotype is associated with RFS and could be an additional prognostic factor for B-ALL patients.
Contrasting Hygiene-Related Gastrointestinal Infections and Stress-Related Diseases at a Primary Health Care Facility within a Sub-Saharan African City: Before and during the COVID-19 Pandemic
Authors: Tetteh-Quarcoo Patience B. , Afutu Emmanuel , Wiafe-Ansong Madonna , Kotey Fleischer C. N. , Dayie Nicholas T. K. D. , Donkor Eric S. , Ahenkorah John , Udofia Emilia Asuquo , Ayeh-Kumi Patrick F. , Dzudzor Bartholomew , Asiedu-Gyekye Isaac Julius
Published: December 2022

Background: With the advent of the COVID-19 pandemic caused by SARS-CoV-2, protocols such as social distancing and upscaling of hygiene practices were implemented to limit the spread of the disease. Meanwhile, along with COVID-19 came stress due to restrictions on movement, trade and transport, and closure of schools, among others. Aim: This study compared the prevalence of hygiene-related gastrointestinal infections and stress-related diseases before (March 2019–February 2020) and during (March 2020–February 2021) the COVID-19 pandemic. Methodology: This was a retrospective single-center review of deidentified patient data from the Korle Bu Polyclinic, Accra, Ghana. Results: Comparing the pre-COVID-19 era to the COVID-19 era, there was a statistically nonsignificant change in the number of cases and prevalence of gastroenteritis and enteric fever (p = 0.084 and 0.081, respectively), although for gastroenteritis, the prevalence was higher for the pre-COVID-19 era compared to during COVID-19 by 1.8 per 1000 cases, while that of enteric fever was higher during the COVID-19 era compared to the pre-COVID-19 era by 1.0 per 1000 cases. Of the stress-related diseases, statistically significant increases in the prevalence of anxiety disorders (p = 0.028), insomnia (p = 0.001), and headache (p = 0.010), were noted, with 2.3, 5.5, and 2.4 per 1000 cases, respectively. There were more female cases than male cases recorded for depression (p = 0.001), headache (p = 0.010), and hypertension (p = 0.001) during the pandemic, and these were statistically significant. Conclusion: During the pandemic, a significant increase in the prevalence of stress-related diseases was observed. However, a statistically nonsignificant change was recorded for gastrointestinal infections, with females reporting more of these disorders. Consequently, it is important to strengthen the capacity for managing stress-related conditions alongside diseases that cause pandemics when they arise.
Warehouse Drone: Indoor Positioning and Product Counter with Virtual Fiducial Markers
Authors: Ekici Murat , Seçkin Ahmet Çağdaş , Özek Ahmet , Karpuz Ceyhun
Published: December 2022

The use of robotic systems in logistics has increased the importance of precise positioning, especially in warehouses. The paper presents a system that uses virtual fiducial markers to accurately predict the position of a drone in a warehouse and count items on the rack. A warehouse scenario is created in the simulation environment to determine the success rate of positioning. A total of 27 racks are lined up in the warehouse and in the center of the space, and a 6 × 6 ArUco type fiducial marker is used on each rack. The position of the vehicle is predicted by supervised learning. The inputs are the virtual fiducial marker features from the drone. The output data are the cartesian position and yaw angle. All input and output data required for supervised learning in the simulation environment were collected along different random routes. An image processing algorithm was prepared by making use of fiducial markers to perform rack counting after the positioning process. Among the regression algorithms used, the AdaBoost algorithm showed the highest performance. The R2 values obtained in the position prediction were 0.991 for the x-axis, 0.976 for the y-axis, 0.979 for the z-axis, and 0.816 for the γ-angle rotation.
Model Predictive Control Technique for Ducted Fan Aerial Vehicles Using Physics-Informed Machine Learning
Authors: Manzoor Tayyab , Pei Hailong , Sun Zhongqi , Cheng Zihuan
Published: December 2022

This paper proposes a model predictive control (MPC) approach for ducted fan aerial robots using physics-informed machine learning (ML), where the task is to fully exploit the capabilities of the predictive control design with an accurate dynamic model by means of a hybrid modeling technique. For this purpose, an indigenously developed ducted fan miniature aerial vehicle with adequate flying capabilities is used. The physics-informed dynamical model is derived offline by considering the forces and moments acting on the platform. On the basis of the physics-informed model, a data-driven ML approach called adaptive sparse identification of nonlinear dynamics is utilized for model identification, estimation, and correction online. Thereafter, an MPC-based optimization problem is computed by updating the physics-informed states with the physics-informed ML model at each step, yielding an effective control performance. Closed-loop stability and recursive feasibility are ensured under sufficient conditions. Finally, a simulation study is conducted to concisely corroborate the efficacy of the presented framework.
Sedimentation Rate and Contamination Levels Profile of Potentially Toxic Elements in the Limoncocha Lagoon RAMSAR Wetland in the Ecuadorian Amazon
Authors: Coral-Carrillo Katty , Ruiz-Gutiérrez Gema , Gómez-Arozamena José , Viguri Javier R.
Published: December 2022

The aim of this study is to analyze the recent sedimentation rate in the center of the Limoncocha lagoon, a Ramsar site in the Ecuadorian Amazon, using the 210Pb dating method and identifying the potentially toxic elements along a 50 cm sediment core. A strategy based on the application of three single and four integrated indices is used to evaluate trace element contamination with depth. Single indices show mainly As and Mo, and Cu, Ba, Cd, Ni, and Pb to a lesser extent, as responsible elements of a minor enrichment between −10 and −40 cm. The multielement slight pollution shows a mixture of potential contamination sources, probably due to agricultural, oil activities, and urban wastewater discharges. However, integrated indices applied, classify the complete core as without potential risk. The 210Pbexcess profile shows three differentiated sections. A surface section where new materials with lower concentrations have been found, probably due to the underground currents that connect the lagoon and the nearby Napo River; a central section where CF-CS model and mass accumulation rate calculations provide a sediment accumulation rate of 0.56 ± 0.03 cm y−1; finally, a deeper section with a constant 210Pbexces profile, showing sediment reworking probably due to local flooding’s.
Phytopathogenic Bacteria Associated with Bacterioses of Common Oak (Quercus robur L.) in Ukraine
Authors: Kulbanska Ivanna , Shvets Marina , Goychuk Anatoliy , Sporek Monika , Pasichnyk Lidiia , Patyka Volodymyr , Kalinichenko Antonina , Bąk Mirosław
Published: December 2022

Studying the biological characteristics of the causative agents of bacteriosis of the common oak (Quercus robur L.) (to highlight the links between the key factors of pathogenesis and bacterial groups) is a new and relevant area of research. This work aims to identify the species composition of phytopathogenic bacteria associated with common oak bacteriosis and to study the morphological, cultural, and biochemical properties of pathogens. To establish the general phytosanitary state of the surveyed stands, classical forest inventory and phytopathological (visual on based on typical symptoms of the disease and macroscopic) methods were used. The properties of isolated bacteria were studied using microbiological methods. The weakening and dieback of common oak in the forests of Ukraine is directly related to four types of bacterioses and their pathogens: the soft bacterial rot of acorns (Pectobacterium carotovorum subsp. carotovorum, bacterial wetwood (Lelliottia nimipressuralis), dry rot of branches and trunks (Erwinia rhapontici), and canker disease (Pseudomonas fluorescens and Pseudomonas sp.). Research on the correspondence between the biological properties of common oak bacteriosis pathogens has established that the synthesis of pectin-degrading enzymes and the ability to ferment with the release of acid and gas in many carbohydrate media by phytopathogenic bacteria is the main characteristic. This ensures the intensity and typical symptoms of the pathogenesis of a particular bacteriosis.
Life Cycle Assessment of a Road Transverse Prestressed Wooden–Concrete Bridge
Authors: Mitterpach Jozef , Fojtík Roman , Machovčáková Eva , Kubíncová Lenka
Published: December 2022

Through its anthropogenic activities in construction, human society is increasingly burdening the environment with a predominantly adverse impact. It is essential to try to use building materials that allow us to build environmentally friendly buildings. Therefore, this article deals with the determination of the environmental performance of a cross-prestressed timber-reinforced concrete bridge using life cycle assessment (LCA) compared with a reinforced concrete road bridge with a similar span and load. The positive environmental performance of the wooden concrete bridge was proved, with a relatively small (22.9 Pt) total environmental damage. The most significant impact on the environment is made by the wood–concrete bridge materials in three categories of impacts: Respiratory inorganics (7.89 Pt, 79.94 kg PM2.5 eq), Global warming (7.35 Pt, 7.28 × 104 kg CO2 eq), and Non-renewable energy (3.96 Pt, 6.01 × 105 MJ primary). When comparing the wood–concrete and steel concrete road bridge, a higher environmental performance of 28% per m2 for the wood–concrete bridge was demonstrated. Based on this environmental assessment, it can be stated that knowledge of all phases of the life cycle of building materials and structures is a necessary step for obtaining objective findings of environmental damage or environmental benefits of building materials or structures.
Remote Sensing-Based Monitoring of Postfire Recovery of Persistent Shrubs: The Case of Juniperus communis in Sierra Nevada (Spain)
Authors: Blanco-Sacristán Javier , Guirado Emilio , Molina-Pardo José Luis , Cabello Javier , Giménez-Luque Esther , Alcaraz-Segura Domingo
Published: December 2022

Wildfires affect the structure, functioning, and composition of ecosystems. Long-term monitoring of the occurrence, abundance, and growth of plant species is key to assessing the responses of the dynamics of plant populations with regard to environmental disturbances, such as wildfires. In this work, we evaluated the changes in the number of individuals and the canopy cover extent of a population of Juniperus communis L. during a four-decade period following a wildfire in a Mediterranean high-mountain ecosystem (Sierra Nevada, Spain). To do this, we used object-based image analysis (OBIA) applied to very high-resolution aerial images. Our study also provides a new approach to optimize the shrub identification process and to semi-automatically evaluate the accuracy of the number of shrubs and their canopy cover. From the 752 individuals present in 1977, only 433 remained immediately after a fire (1984), a few more disappeared one decade later (420 shrubs in 1997), while by 2008, the population had partially recovered to 578 shrubs. The wildfire decreased juniper canopy cover from 55,000 m2 to 40,000 m2, but two decades later it had already recovered to 57,000 m2. The largest shrubs were more resistant to fire than the smallest ones and recovered in a shorter time period. The protection measures introduced with the park declaration seemed to have contributed to the post-fire recovery. The potential of this methodology in the management and conservation of biodiversity in the future is also discussed.
Understanding the Effects of Self-Induced Anaerobic Fermentation on Coffee Beans Quality: Microbiological, Metabolic, and Sensory Studies
Authors: da Silva Vale Alexander , Balla Gabriel , Rodrigues Luiz Roberto Saldanha , de Carvalho Neto Dão Pedro , Soccol Carlos Ricardo , de Melo Pereira Gilberto Vinícius
Published: December 2022

In this study, an investigation of the microbial community structure and chemical changes in different layers of a static coffee beans fermentation tank (named self-induced anaerobic fermentation—SIAF) was conducted at different times (24, 48, and 72 h). The microbial taxonomic composition comprised a high prevalence of Enterobacteriaceae and Nectriaceae and low prevalence of lactic acid bacteria and yeast, which greatly differs from the traditional process performed in open tanks. No major variation in bacterial and fungal diversity was observed between the bottom, middle, and top layers of the fermentation tank. On the other hand, the metabolism of these microorganisms varied significantly, showing a higher consumption of pulp sugar and production of metabolites in the bottom and middle layers compared to the top part of the fermentation tank. Extended processes (48 and 72 h) allowed a higher production of key-metabolites during fermentation (e.g., 3-octanol, ethyl acetate, and amyl acetate), accumulation in roasted coffee beans (acetic acid, pyrazine, methyl, 2-propanone, 1-hydroxy), and diversification of sensory profiles of coffee beverages compared to 24 h of fermentation process. In summary, this study demonstrated that SIAF harbored radically different dominant microbial groups compared to traditional coffee processing, and diversification of fermentation time could be an important tool to provide coffee beverages with novel and desirable flavor profiles.
Formulation Development and In Vitro/In Vivo Characterization of Methotrexate-Loaded Nanoemulsion Gel Formulations for Enhanced Topical Delivery
Authors: Latif Muhammad Shahid , Nawaz Asif , Asmari Mufarreh , Uddin Jalal , Ullah Hidayat , Ahmad Saeed
Published: December 2022

Methotrexate-loaded oil-in-water nanoemulsion formulations were prepared using the high shear homogenization technique. A drug excipient study (ATR-FTIR) was carried out to investigate the compatibility between the drug, the polymers, and its admixtures. The thermal stability of the nanoemulsion formulations was evaluated by subjecting them to a heating and cooling cycle. The prepared nanoemulsion formulations (FNE1 to FNE6) were evaluated for particle size, PDI value, and entrapment efficiency (EE). They were analyzed for morphological information using transmission electron microscopy. The drug (methotrexate)-loaded nanoemulsion formulations (FNE2, FNE4, and FNE6) were then converted into nanoemulsion gel formulations by adding 1% chitosan (polymer) as a gelling agent. The nanoemulsion gel formulations (FNEG2, FNEG4, and FNEG6) were investigated for physicochemical parameters, viscosity, spreadability, extrudability, drug content, and skin irritation. Various penetration enhancers (olive oil, clove, and almond oil) were employed to examine the potency of the prepared nanoemulsion gel formulations. In vitro drug release, ex vivo permeation, skin drug retention, and stability tests were carried out for evaluation of the prepared nanoemulsion gel formulations (FNEG2, FNEG4, and FNEG6). The data obtained from the in vitro study were subjected to the kinetic model, and the Korsemeyer–Peppas model was best fitted to the data. The nanoemulsion gel formulation FNEG6 showed the maximum controlled drug release and followed an anomalous, non-Fickian release mechanism. The use of almond oil in the preparation of the nanoemulsion gel formulation FNEG6 helped the penetration of the drug across stratum corneum and the restructuring of the properties of skin and resulted in a higher penetration and retention of methotrexate in a deeper layer of the skin. The current study concluded that the methotrexate-loaded nanoemulsion gel formulation FNEG6 showed the best optimum release, permeation, and retention results as compared to the available oral tablets’ formulations, followed by a low serum concentration and the maximum drug retention, which is beneficial in treating skin infections and reducing systemic toxicity.
Microanalytical Investigation of Prehistoric Colorants from Uralian Rock Art (Ignatievskaya Cave and Idrisovskaya II and Zmiev Kamen’ Pictographs)
Authors: Kiseleva Daria , Shagalov Evgeny , Pankrushina Elizaveta , Shirokov Vladimir , Khorkova Arina , Danilov Danil
Published: December 2022

Uralian parietal and rock art (cave paintings and pictographs, or “pisanitsy”) represents a unique archaeological and cultural phenomenon, comprising 90 sites stretching for more than 800 km from north to south, which date from the Paleolithic era extending into the present Holocene epoch. The identification of the nature of prehistoric colorants provides an insight into their provenance, manufacture and utilization, as well as contributing to the conservation and restoration of drawings. The studies of mineral, elemental and organic phase composition of the colorant micro-samples from the drawings of Ignatievskaya cave and Idrisovskaya II and Zmiev Kamen’ pictographs (Southern and Middle Urals, Russia) discussed in the present work were carried out using a special set of microspectroscopic methods (SEM-EDS and Raman spectroscopy) offering high spatial resolution. The fatty acid composition of the organic phase was analyzed by GC–MS. The technology of colorant manufacture could have included thorough grinding and mixing of unheated hematite with an organic binder made from animal fat and a clayey extender in order to achieve the desired hue and intensity of the color. It is possible that the colorant was applied in layers (Idrisovskaya II and Zmiev Kamen’ pictographs). The development of authigenic phosphate and sulfate (gypsum) mineralization, which is observed in all studied sites, as well as oxalate encrustation on the Idrisovskaya II pictograph, indicates the conditions and processes of secondary mineral formation.
Specific Changes in Morphology and Dynamics of Plant Mitochondria under Abiotic Stress
Authors: Tang Hui , Zhu Hongliang
Published: December 2022

As the global climate continues to warm and the greenhouse effect intensifies, plants are facing various abiotic stresses during their growth and development. In response to changes in natural environment, plant mitochondria regulate their functions through morphological and dynamic changes. Mitochondria are highly dynamic organelles with the ability to continuously cleavage and fuse, regulating dynamic homeostatic processes in response to the needs of organism growth and the changes in external environmental conditions. In this review, we introduced the structure of the outer and inner mitochondrial membrane and discussed the relevant factors that influence the morphological changes in mitochondria, including proteins and lipids. The morphological and dynamic changes in mitochondria under various abiotic stresses were also revisited. This study aims to discuss a series of changes in plant mitochondrial ultrastructure under abiotic stress. It is very important that we analyze the association between plant mitochondrial functions and morphological and dynamic changes under stress to maintain mitochondrial homeostasis and improve plant stress resistance. It also provides a new idea for plant modification and genetic breeding under the dramatic change in global natural environment.
Plant Growth, Yield, and Quality of Containerized Heirloom Chile Pepper Cultivars Affected by Three Types of Biostimulants
Authors: Arthur Jacob D. , Li Tongyin , Bi Guihong
Published: December 2022

Peppers (Capsicum annuum) are one of the most widely cultivated vegetable crops, with per capita consumption of bell and chile peppers being 11.4 and 7.7 pounds in 2017. Biostimulants are an emerging sustainable alternative to enhance plant health by increasing photosynthetic activity, stress tolerance, and nutrient uptake through various modes of action. The effects of different biostimulant applications largely remain unknown in containerized heirloom pepper production. This study evaluated plant growth, yield, and fruit quality of nine heirloom chile pepper cultivars, including ‘Anaheim Chili,’ ‘Ancho or Poblano,’ ‘Big Jim,’ ‘Cayenne Purple,’ ‘Chile de Arbol,’ ‘Jamaica Hot Red,’ ‘Mulato Isleno,’ ‘Padron,’ and ‘Pasilla Bajio’ in a container production system throughout the years of 2020 and 2021. Each cultivar was treated with three types of biostimulants, including Tribus® Original (a mixture of Bacillus bacterias), Vitazyme (containing plant growth regulators and B vitamins), C-Bio CPS (seaweed extract of Ascophyllum Nodosum), and water as control. Pepper cultivars varied in yield and quality, including fruit length, diameter, single fruit weight, and fruit color in both years. ‘Anaheim Chili,’ ‘Big Jim,’ and ‘Jamaica Hot Red’ produced highest marketable yields similarly, with ‘Chile de Arbol’ and ‘Pasilla de Bajio’ producing the lowest marketable yields in both years. Biostimulant application did not affect marketable yield either in 2020 or 2021 but enhanced fruit quality, including fruit length, diameter, and green coloration.
Evolution of the Volatile Organic Compounds, Phenols and Antioxidant Capacity during Fruit Ripening and Development of Rubus ulmifolius Schott Fruits
Authors: Castro Ricardo I. , Vásquez-Rojas Carlos , Cortiella Mariona Gil I , Parra-Palma Carolina , Ramos Patricio , Morales-Quintana Luis
Published: December 2022

Nowadays, a growing interest in consumers’ fruit with a high content of health-promoting compounds has been observed. In this sense, wild berries have received special attention based on their high accumulation of phenolic compounds, as well as their characteristic and pleasant aroma. In this work, we characterize the color development, antioxidant capacity, phenolic contents, and volatile profile of Rubus ulmifolius Schott fruit at different ripening stages during two seasons on the same orchard. Four stages were established based on the color parameter, which was consistent with changes in the weight and size of the fruit. In addition, total phenolic and flavonoid content showed a decrease during the fruit ripening, in contrast with the total anthocyanins content that increased at the final stages of ripening. In addition, the antioxidant capacity was evaluated through two approaches: FRAP and DPPH, which consistently displayed higher levels at the final stages in the two different seasons. Finally, the VOCs analysis showed an active synthesis of volatile compounds during the late stage of ripening, with alcohols being the most abundant compounds for each ripening stage. These results allow us to propose a classification of different ripening stages of the wild blackberry to have a better knowledge of this interesting fruit with higher healthy- and nutraceutical compounds.
Academic Perfectionism, Psychological Well-Being, and Suicidal Ideation in College Students
Authors: Fernández-García Olga , Gil-Llario María Dolores , Castro-Calvo Jesús , Morell-Mengual Vicente , Ballester-Arnal Rafael , Estruch-García Verónica
Published: December 2022

High levels of perfectionism in college students can compromise their academic performance and psychological well-being. This study aims to analyze the implication of perfectionism in psychological well-being and suicidal ideation in the last year. A total of 1.287 students from different degrees reported their academic performance in the previous academic year and completed questionnaires on academic perfectionism, psychological well-being, and suicidal thoughts in the last year. In both men and women, academic perfectionism correlates positively with academic performance and negatively with the different dimensions of psychological well-being. Likewise, both the average grade in the previous year (β = 0.364) and the level of demand of the degree (β = −0.461) are mediating variables between perfectionism and psychological well-being. Furthermore, the interaction between perfectionism and academic performance is related positively to psychological well-being, but not to with suicidal ideation. So, the relation between suicidal ideation and perfectionism is positive (OR = 1.075), but this is negative with academic performance (OR = 0.900), although both variables show a mild predictive capacity. These findings suggest that the levels of perfectionism are associated differently with the mental health of students, since if perfectionism is effective (high academic performance), psychological well-being is high, although in our results, this interaction is not as important for suicidal thoughts.
Human Microbiome Mixture Analysis Using Weighted Quantile Sum Regression
Authors: Eggers Shoshannah , Bixby Moira , Renzetti Stefano , Curtin Paul , Gennings Chris
Published: December 2022

Studies of the health effects of the microbiome often measure overall associations by using diversity metrics, and individual taxa associations in separate analyses, but do not consider the correlated relationships between taxa in the microbiome. In this study, we applied random subset weighted quantile sum regression with repeated holdouts (WQSRSRH), a mixture method successfully applied to ‘omic data to account for relationships between many predictors, to processed amplicon sequencing data from the Human Microbiome Project. We simulated a binary variable associated with 20 operational taxonomic units (OTUs). WQSRSRH was used to test for the association between the microbiome and the simulated variable, adjusted for sex, and sensitivity and specificity were calculated. The WQSRSRH method was also compared to other standard methods for microbiome analysis. The method was further illustrated using real data from the Growth and Obesity Cohort in Chile to assess the association between the gut microbiome and body mass index. In the analysis with simulated data, WQSRSRH predicted the correct directionality of association between the microbiome and the simulated variable, with an average sensitivity and specificity of 75% and 70%, respectively, in identifying the 20 associated OTUs. WQSRSRH performed better than all other comparison methods. In the illustration analysis of the gut microbiome and obesity, the WQSRSRH analysis identified an inverse association between body mass index and the gut microbe mixture, identifying Bacteroides, Clostridium, Prevotella, and Ruminococcus as important genera in the negative association. The application of WQSRSRH to the microbiome allows for analysis of the mixture effect of all the taxa in the microbiome, while simultaneously identifying the most important to the mixture, and allowing for covariate adjustment. It outperformed other methods when using simulated data, and in analysis with real data found results consistent with other study findings.
Empirical Study on the Impact of Government Environmental Subsidies on Environmental Performance of Heavily Polluting Enterprises Based on the Regulating Effect of Internal Control
Authors: Pei Weiqi , Pei Weiran
Published: December 2022

At present, China’s economy is developing rapidly; however, at the same time, it is also bringing more and more serious environmental problems. Although many laws have been established in the country to limit pollution by enterprises—and enterprises are actively saving energy and reducing pollution—the situation is still not optimistic. In 2016, there were 35 cities across the country that exceeded the annual average air quality standard; these regions have been plagued by haze for a long time, which seriously threatens people’s health and sustainable social and economic development. Therefore, while pursuing economic benefits and realizing greater value, importance must be attached to environmental performance, especially for enterprises with serious pollution. Using the panel regression analysis method, based on the data of enterprises from 2010 to 2019, this paper empirically analyzes the effects of government environmental protection subsidies and internal control quality on the environmental performance of heavily polluting enterprises, and verifies the nonlinear critical effect of government subsidies and internal control on environmental performance. In addition, this paper also uses the intermediate effect model to verify the mechanism by which environmental subsidies impact the environmental performance of heavily polluting enterprises. Through the empirical analysis, the effects of environmental protection subsidies and internal control on the environmental performance of enterprises with serious pollution are obtained. In the process of environmental subsidy affecting the environmental performance of heavy-polluting enterprises, internal control plays a key intermediary role. In addition, environmental subsidies and internal controls have a certain impact on the environmental performance of heavily polluting enterprises. Through the empirical analysis, it is concluded that there are obvious differences between government subsidies and internal control on the environmental governance effects of heavily polluting enterprises in different regions and with different property rights. Among them, the environmental protection subsidies have the greatest impact on pollution degree in the central region, followed by the eastern region; in the western region, it is not obvious. Secondly, the incentive effect of government environmental subsidies on state-owned enterprises with serious environmental pollution is better than the environmental governance effect of non-state-owned enterprises.
Satisfaction Level and Performance of Physiotherapy Students in the Knowledge of Musculoskeletal Disorders through Nearpod: Preliminary Reports
Authors: Vinolo-Gil Maria Jesus , García-Campanario Ismael , Lagares-Franco Carolina , Gonzalez-Medina Gloria , Rodríguez-Huguet Manuel , Martín-Vega Francisco Javier
Published: December 2022

Physiotherapists are at high risk for musculoskeletal disorders. There is a need in academia to address workers’ health issues at the time of graduation. Nearpod is an educational application founded on a web-based learning tool. In the field of Health Sciences, the use of Nearpod has been scarce. The objective of this study was to determine the level of satisfaction with using this interactive tool and to assess the influence of using Nearpod in class on students’ performance while dealing with the topic of musculoskeletal disorders in third-year Degree in Physiotherapy students during the 2021–2022 academic year. The participants were students at the University of Cadiz. They were randomly divided into two groups, a control group using a PowerPoint presentation and an experimental group using the interactive Nearpod application. The experimental group took two surveys to determine their satisfaction with the method used. Students also took a multiple-choice test to assess the knowledge acquired. In the surveys, a high percentage of satisfaction was obtained (97.62% and 99.39%). There were no significant differences in the scores obtained by the two groups, although there were significant differences in response time in favor of the experimental group (p = 0.022). Although studies should be carried out with larger samples and on different subjects, it seems that Nearpod is a tool with great potential for teaching the study of musculoskeletal disorders.
Patient Safety and the COVID-19 Pandemic in Germany: A Repeated Population-Based Cross-Sectional Survey
Authors: Amberger Olga , Müller Angelina , Lemke Dorothea , Müller Hardy , Schwappach David , Wendt Peter , Wensing Michel , Brueckle Maria-Sophie , Müller Beate S.
Published: December 2022

The coronavirus (COVID-19) has presented Germany with major challenges and has led to concerns about patient safety. We conducted an observational, population-based, nationwide, repeated cross-sectional survey on patient safety in Germany in 2019, 2020, and 2021. Each of the three samples consisted of 1000 randomly recruited adults. Self-reported data via computer-assisted telephone interviews were taken from TK Monitor of Patient Safety. Perceptions, experience, and knowledge relating to patient safety were assessed. The majority of respondents considered medical treatment to involve risks to patient safety. This proportion decreased during the pandemic. The majority also had a high degree of self-efficacy regarding the prevention of medical errors, whereby the percentage that felt well informed with regard to patient safety rose throughout the pandemic. The proportion of persons that suspected they had in the past experienced an error in their treatment remained steady at one third as well as the reported errors. In 2020, 65% of respondents thought health communication with service providers (e.g., extent and comprehensibility of information) remained unchanged during the pandemic, while 35% reported that medical appointments had been cancelled or postponed. This study is the first to assess patient safety from a general population perspective during the coronavirus pandemic in Germany. COVID-19 had a positive impact on perceived patient safety but no impact on suspected and reported errors. Self-efficacy with regard to medical error prevention steadily increased in the general population, and people considered themselves well informed.
Elevational Pattern of Leaf Mine Diversity on Quercus variabilis Blume at Baotianman, Henan, China
Authors: Chen Xiaona , Zhong Miao , Cui Lixing , Xu Jiasheng , Dai Xiaohua , Liu Xiaojing
Published: December 2022

The species composition and diversity pattern of leaf miners on dominant trees in China are poorly understood. Using Hill-based diversity metrics, the elevational patterns of taxonomic, phylogenetic, and functional diversity for leaf miners on Quercus variabilis Blume at Baotianman were systematically analyzed. Leaf mine types belonged to ten genera and seven families. Different leaf miners had different elevational preferences. Most taxonomic and phylogenetic Hill diversity indices had typical hump-shaped elevational patterns, with a peak at the middle elevation of approximately 875 m. No functional Hill diversity indices presented significant linear or nonlinear trends with altitude. The driving factors behind the elevational distribution patterns of leaf miners require further work.
An RNA Virome Analysis of the Pink-Winged Grasshopper Atractomorpha sinensis
Authors: He Yu-Juan , Ye Zhuang-Xin , Zhang Chuan-Xi , Li Jun-Min , Chen Jian-Ping , Lu Gang
Published: December 2022

A large number of RNA viruses have been discovered in most insect orders using high-throughput sequencing (HTS) and advanced bioinformatics methods. In this study, an RNA virome of the grasshopper was systematically identified in Atractomorpha sinensis (Orthoptera: Pyrgomorphidae), an important agricultural pest known as the pink-winged grasshopper. These insect viruses were classified as the nege-like virus, iflavirus, ollusvirus, and chu-like virus using HTS and phylogenetic analyses. Meanwhile, the full sequences of four novel RNA viruses were obtained with RACE and named Atractomorpha sinensis nege-like virus 1 (ASNV1), Atractomorpha sinensis iflavirus 1 (ASIV1), Atractomorpha sinensis ollusvirus 1 (ASOV1), and Atractomorpha sinensis chu-like virus 1 (ASCV1), respectively. Moreover, the analysis of virus-derived small interfering RNAs showed that most of the RNA viruses were targeted by the host antiviral RNA interference pathway. Moreover, our results provide a comprehensive analysis on the RNA virome of A. sinensis.
QFD and SDE Methods Applied to Autonomous Minibus Redesign and an Innovative Mobile Charging System (MBS)
Authors: Frizziero Leonardo , Donnici Giampiero , Galiè Giulio , Pala Giacomo , Pilla Martina , Zamagna Elia
Published: December 2022

Urban mobility scenarios are constantly evolving, and today’s solutions may not be adequate in the future. Through innovative analysis and design methods encapsulated by the IDeS methodology, it is possible to plausibly hypothesize a number of key scenarios to be analyzed, for which vehicles can be designed in order to solve the main problems. Scenarios such as the steady growth in public mobility, based on the sharing of electric mini-buses at the expense of the privatization of the means of transport, lead to the gradual rethinking of citizens’ needs and the supporting infrastructure. Problems such as the lack of privacy of public vehicles, the efficiency of the infrastructure and recharging modes of e-buses, and autonomous driving are addressed here through methods such as QFD (quality function deployment) and SDE (stylistic design engineering), with the aim of outlining a proposal that, to date, is futuristic but is designed to be concrete and feasible within the next decade. These methodologies were applied to the design of a sustainable urban transport system consisting of an electric mini-bus, effected by rethinking the layout of the interior spaces in favor of areas enabling greater privacy and a mobile recharging system (MBS) capable of offering a new management strategy for the non-stop recharging phase. Through the use of an MBS, which functions as a mobile ‘energy bank’ module that is capable of autonomously reaching a mini-bus in need of recharging and extending its autonomy by connecting and recharging it, the proposed system can potentially be enabled to perform its required service during the day without any need to spend time making intermediate stops for the purpose of recharging.
Plant-Pathogenic Fusarium Species
Authors: Stępień Łukasz
Published: December 2022

Fusarium species are ubiquitous fungi, both saprotrophic and pathogenic to plants, animals and humans. They are also potent mycotoxin producers which makes them one of the most devastating plant pathogens. Mycotoxin biosynthesis and regulation has recently become one of the mainstream research topics, since knowledge concerning individual metabolic pathways became available and modern ’omics’ techniques allowed us to expand this even further. Independently, high-throughput sequencing methodology helped researchers gain insight into the complex phylogenetic relationships among closely related genotypes comprising Fusarium populations, species and species complexes. Molecular tools have so far been very powerful in species identification and phylogeny, as the great diversity of the Fusarium genus has forced scientists to continuously revise previously described taxons.
Endophytic Fungus Negatively Affects Salt Tolerance of Tall Fescue
Authors: Kalske Aino , Saikkonen Kari , Helander Marjo
Published: December 2022

Vertically transmitted endophytic fungi can mitigate the negative effects of salinity encountered by their host grass and alter the competitive interactions between plant individuals. To experimentally study the interactive effects of the fungal endophyte Epichloë coenophiala on salt tolerance and intraspecific competition of its host plant, tall fescue Festuca arundinacea, we subjected 15 maternal lines of each Epichloë associated (E+) and Epichloë free (E−) tall fescue to salt treatment and competition in the greenhouse and common garden. Then, to explore variation in endophyte incidence in natural populations of tall fescue, we surveyed 23 natural populations occurring on or near the Baltic Sea coast in Aland islands in southwestern Finland for endophyte incidence, distance to shore, and competitive environment. Under salinity in the greenhouse, E− plants grew larger than E+ plants, but there was no size difference in the control treatment. E− plants grew taller and were more likely to flower than E+ plants when grown in benign conditions in the common garden but not with salinity or competition. The frequency of Epichloë incidence was high (90%) in natural populations, and it decreased towards the shore and risk of salt exposure. These results demonstrate a negative effect of Epichloë endophyte on the salt tolerance of its host. The high incidence of Epichloë in natural populations of tall fescue in the northern part of the species distribution range is likely due to factors other than salinity.
Discovery and Validation of a SIT1-Related Prognostic Signature Associated with Immune Infiltration in Cutaneous Melanoma
Authors: Jia Ming , Liu Chengfei , Liu Yuean , Bao Zhengqiang , Jiang Yuhua , Sun Xifeng
Published: December 2022

Signaling threshold regulating transmembrane adaptor 1 (SIT1) encodes a disulfide-linked homodimeric lymphocyte-specific glycoprotein involved in immune cell activation. However, the relationship between SIT1 and the prognosis of skin cutaneous melanoma (SKCM) and tumor-infiltrating lymphocytes remains elusive. Here, we first compared the differences in SIT1 expression levels between SKCM tissues and adjacent normal tissues. Next, we found that the immune cell infiltration levels and signature pattern of immune infiltration were positively associated with the SIT1 gene mRNA levels. TCGA_SKCM RNA-seq data unveiled that the SIT1 upregulated several immune-associated signaling pathways in GSEA analysis. The high expression of SIT1 was closely related to improved survival in patients with SKCM. A pathway enrichment analysis of SIT1-associated immunomodulators indicated the involvement of the NF-κB signaling pathways. Based on SIT1-associated immunomodulators, we built a 13-gene signature by LASSO Cox regression which served as an independent prognostic factor for the survival of melanoma patients. By using the signature risk score, we achieved a good prediction result for the immunotherapy response and survival of SKCM patients. Our findings provided evidence for SIT1’s implication in tumor immunity and survival of SKCM patients. The nominated immune signature is a promising predictive model for prognosis and immunotherapy sensitivity in SKCM patients.
Genetic Predictors of the Development of Complications after Coronary Stenting
Authors: Taizhanova Dana , Kalimbetova Akerke , Bodaubay Roza , Toleuova Aliya , Toiynbekova Rakhima , Beysenbekova Zhazira , Visternichan Olga , Tauesheva Zauresh , Kadyrova Irina , Babenko Dmitriy , Akhmaltdinova Lyudmila , Kolesnichenko Svetlana , Kolesnikova Yevgeniya , Avdienko Olga V. , Akilzhanova Ainur , Gerotziafas Grigorios T.
Published: December 2022

Due to the fact that there are scientific discussions about the significance of gene polymorphisms in the risk of developing cardiovascular complications after a percutaneous coronary intervention, it is of interest to evaluate the genetic predictors of the development of cardiovascular events. This study is a molecular genetic study. Association with the genes of biomarkers for inflammation and immune response increases the risk of cardiovascular events: rs1234313 (TNFSF4): (A/G, OR-4.57 (2.35–8.87), p ≤ 0.0001), (A/G-A/A, OR-3.14 (1.75–5.63), p ≤ 0.0001), and (A/G, OR = 4.01 (2.19–7.36), p ≤ 0.0001); rs3184504 (SH2D3); ATXN2: (C/T, OR-2.53 (1.28–5.01), T/T, OR-2.99 (1.13–7.92), p = 0.017)), (C/T-T/T, OR-2.61 (1.35–5.07), p = 0.000), and (OR-1.89 (1.15–3.09), p = 0.009)). According to the lipid metabolism biomarker genes, rs2943634: (A/C OR-2.57 (1.18–5.62), p = 0.013); according to the endothelial biomarker genes, rs2713604: (DNAJB8-AS1; GATA2): (C/T, OR-4.27 (2.35–7.76), p ≤ 0.0001), (C/T-C/C, OR-4.13 (2.31–7.40), p ≤ 0.0001), (OR-4.05 (2.24–7.30), p ≤ 0.0001), and (C/T, OR-3.46 (1.99–6.00), p ≤ 0.0001). The regression analysis found that in the presence of the rs2943634 gene polymorphism, the risk of late cardiovascular events increases by 4.007 times with 95% CI (1.502:10.692), p = 0.006. The genes of biomarkers for the risk of cardiovascular events are rs1234313(TNFSF4), rs3184504 (SH2D3; ATXN2), rs2943634, and rs2713604 (DNAJB8-AS1; GATA2). The only predictor of the development of new cardiovascular events was rs2943634, which belongs to the group of lipid metabolism biomarkers.
Vapor Pressure versus Temperature Relations of Common Elements
Authors: Mondal B. , Mukherjee T. , Finch N. W. , Saha A. , Gao M. Z. , Palmer T. A. , DebRoy T.
Published: December 2022

The vapor pressure values of common elements are available in the literature over a limited temperature range and the accuracy and reliability of the reported data are not generally available. We evaluate the reliability and uncertainty of the available vapor pressure versus temperature data of fifty common pure elements and recommend vapor pressure versus temperature relations. By synthesizing the vapor pressure values from measurements reported in the literature with the values computed using the Clausius Clapeyron relation beyond the boiling point, we extend the vapor pressure range from 10−8 atm to 10 atm. We use a genetic algorithm to optimize the fitting of the vapor pressure data as a function of temperature over the extended vapor pressure range for each element. The recommended vapor pressure values are compared with the corresponding literature values to examine the reliability of the recommended values.
Transition-Metal-Based Compounds for Electrochemical Energy Conversion Processes
Authors: Mikuła Andrzej , Kusior Anna
Published: December 2022

The era of ever-growing worldwide energy requirements demands the development of new methods of energy conversion, where the design of novel materials and the improvement of the efficiency of existing ones are of great importance [...]