bluuebunny/crossref_metadata_2025 huggingface dataset:
Prognostic stratification based on the levels of tumor-infiltrating myeloid-derived suppressor cells and PD-1/PD-L1 axis in locally advanced rectal cancer
Authors: Lim Yu Jin , Koh Jaemoon , Choi Minji , Kim Sehui , Chie Eui Kyu
Published: October 2022

Background

Although rectal cancer remains somewhat sanctuary to the contemporary immunotherapy, there is increasing knowledge on clinical implications of anti-tumor immunity. This study evaluated the prognostic relevance of two immune-inhibitory functions, myeloid-derived suppressor cells (MDSCs) and programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) axis.

Methods

Study cohort is comprised of 165 patients with locally advanced rectal cancer who underwent neoadjuvant chemoradiotherapy followed by definitive resection. Using postsurgical tissue microarrays, the number of MDSCs, PD-1+/CD8+ tumor-infiltrating lymphocyte (TIL) ratio, and PD-L1 expression scores in stromal immune cells and tumor cells were assessed.

Results

Positive correlation was observed between the PD-1+/CD8+ TIL ratio and number of MDSCs (P < 0.001). The greater the immune infiltrates, the higher the PD-L1 immune cell score (P < 0.001). MDSCHigh, PD-1+/CD8+ TILHigh, PD-L1 immune cell scoreLow, and PD-L1 tumor H-scoreHigh were associated with worse disease-free survival (DFS) (P < 0.001, P = 0.042, 0.047, and P < 0.001, respectively). To integrate the adverse effects of MDSCHigh, PD-1+/CD8+ TILHigh, and either PD-L1 immune cell scoreLow (set I) or tumor H-scoreHigh (set II), prognostic risks were stratified according to the number of factors: 0, 1, and 2−3 (P < 0.001 for I and II). On multivariate analyses, patients with multiple risk factors for set I and II had worse prognosis (P < 0.001; 2−3 vs. 0 for models I and II), and the two prognostic models had acceptable predictability.

Conclusion

In this study, integration of the prognostic impact of MDSCs and PD-1/PD-L1 stratified the long-term risks of patients with locally advanced rectal cancer. Thus, further exploration could be focused to the identified subset of patients carrying worse prognosis, where potential benefits could be derived by targeting the two components contributing to the immunosuppressive microenvironment.
Shikonin induces ferroptosis in multiple myeloma via GOT1-mediated ferritinophagy
Authors: Li Wenxia , Fu Hangjie , Fang Liuyuan , Chai Hui , Gao Tianwen , Chen Zhenzhen , Qian Shenxian
Published: October 2022

Multiple myeloma (MM) is an incurable hematological malignancy that lacks effective therapeutic interventions. Ferroptosis is a newly discovered form of cell death that has shown great potential for MM therapy. As a proteasome inhibitor and necroptosis inducer, shikonin (SHK) performs dual functions in MM cells. However, whether SHK inhibits the development of MM via ferroptosis or any other mechanism remains elusive. Here, we provide evidence that SHK treatment was capable of inducing ferroptosis and immunogenic cell death (ICD) in MM. The results showed that SHK treatment induced lactate dehydrogenase release, triggered cell death, evoked oxidative stress, and enhanced ferrous iron and lipid peroxidation levels. Furthermore, treatment with ferroptosis inhibitors reversed SHK-induced cell death, which indicated that ferroptosis contributed to this phenomenon. Meanwhile, ferroptosis was accompanied by the extracellular release of Adenosine 5’-triphosphate (ATP) and High mobility group protein B1 (HMGB1), which are characteristics of ICD. Further investigation showed that glutamic-oxaloacetic transaminase 1 (GOT1) acted as a critical mediator of SHK-induced ferroptosis by promoting ferritinophagy. In conclusion, our findings suggest that SHK exerts ferroptotic effects on MM by regulating GOT1-mediated ferritinophagy. Thus, SHK is a potential therapeutic agent for MM.
Analysis of exosomal competing endogenous RNA network response to paclitaxel treatment reveals key genes in advanced gastric cancer
Authors: Lei Jun , Zhang Guifeng , Li Deyu , Zhong Jiangming , Chen Qiao , Lin Li , Liu Zhenhua
Published: October 2022

Background

Exosome is an important component of the tumor immune microenvironment and plays critical role in cancer pathogenesis. The exosome transcriptome of gastric cancer (GC) response to paclitaxel chemotherapy has not been investigated in the past.

Methods

ceRNA microarrays were performed in exosomes from six advanced GC patients before and after paclitaxel treatment. Bioinformatics tools were used to identify differential expressing genes and construct competing endogenous RNA (ceRNA) networks. The importance of hub genes in the ceRNA network was confirmed by survival analysis and functional analysis.

Results

A total of 213 differential mRNAs, 370 lncRNAs, and 376 circRNAs were identified, and hub genes in ceRNA networks were screened. The differential genes were associated with GO terms SNAP complex, gap junction, protein transporter activity, cytokine receptor, and KEGG pathways synaptic vesicle cycle, propanoate metabolism, Epstein–Barr virus infection, heparin, and steroid biosynthesis, and beta-alanine metabolism. ULK2, CYP2R1, BTLA, and miR-105-5p are prognostic genes for overall survival. Paclitaxel may target ULK2 which is involved in mitosis and cell cycle. miR-105-5p may target ULK2 3’UTR.

Conclusion

The work for the first time identified exosomal RNA biomarkers and constructed a ceRNA network in GC response to paclitaxel, revealed novel molecular mechanisms of GC, and provided new candidates for GC diagnosis and treatment.
Circ_0084043-miR-134-5p axis regulates PCDH9 to suppress melanoma
Authors: Cai Guiyue , Zou Ruitao , yang Huizhi , Xie Jiahao , Chen Xiaoxuan , Zheng Chunchan , Luo Sujun , Wei Na , Liu Shuang , Chen Rongyi
Published: October 2022

The low survival rates, poor responses, and drug resistance of patients with melanoma make it urgent to find new therapeutic targets. This study investigated whether the circ_0084043-miR-134-5p axis regulates the antitumor effect of protocadherin 9 (PCDH9) in melanoma. Ectopic expression or knock down (KD) of PCDH9 with a lentivirus vector, we explored its effects on the proliferation, invasion, and apoptosis of melanoma and verified its regulatory effect on ras-related C3 botulinum toxin substrate 1 (RAC1), proline-rich tyrosine kinase 2 (Pyk2), Cyclin D1, matrix metalloproteinase 2 (MMP2), and MMP9. We further observed the effect of KD circ_0084043 on the malignant behavior of melanoma and studied whether circ_0084043 sponged miR-134-5p and regulated PCDH9. We found that circ_0084043 was overexpressed in melanoma and associated with the malignant phenotype. PCDH9 was poorly expressed in human melanoma tissues, and overexpression of PCDH9 inhibited melanoma progression. Quantitative real-time PCR and Western blotting results showed that overexpression of PCDH9 could downregulate RAC1, MMP2, and MMP9 and upregulate Pyk2 and Cyclin D1. Circ_0084043 KD inhibited invasion and promoted apoptosis in melanoma cells. Circ_0084043 could sponge miR-134-5p and thus indirectly regulate PCDH9. Furthermore, we discovered that inhibiting circ_0084043 had an anti–PD-Ll effect. In vivo, PCDH9 overexpression inhibited melanoma tumor growth, but PCDH9 KD promoted it. In conclusion, PCDH9, which is regulated by the circ 0084043-miR-134-5p axis, can suppress malignant biological behavior in melanoma and influence the expression levels of Pyk2, RAC1, Cyclin D1, MMP2, and MMP9.
Long-term nutritional status after total gastrectomy was comparable to proximal gastrectomy but with much less reflux esophagitis and anastomotic stenosis
Authors: Ding Shikang , Zheng Xiaohao , Wang Shenghui , Wu Ming , Wu Yunzi , Sun Chunyang , Yang Lin , Xue Liyan , Wang Bingzhi , Wang Chengfeng , Xie Yibin
Published: October 2022

Aim

To compare the long-term nutritional status, reflux esophagitis and anastomotic stenosis, between total gastrectomy (TG) and proximal gastrectomy (PG).

Methods

Patients who underwent PG or TG in this single institution between January 2014 and December 2016 were included in this study. The inclusion and exclusion criteria were defined. One-to-one propensity score matching (PSM) by the demographic and pathological characteristics was performed to compare the long-term outcomes between the two groups. The primary endpoint was long-term nutritional status, and the second endpoints were reflux esophagitis and anastomotic stenosis. Long-term nutritional status was valued by percentage of body mass index (%BMI), body weight, and blood test including total protein, prealbumin, hemoglobin and total leukocytes.

Results

Totally 460 patients received PG or TG in our institution for the treatment between January 2014 and December 2016 and according to the inclusion and exclusion criteria 226 cases were included in this study finally. There was no significant difference as to nutritional status in the end of first 5 years after PG or TG. While reflux esophagitis and anastomotic stenosis were significantly higher in the PG group than in the TG group (54.4% versus 26.8%, p < 0.001; 14.9% versus 4.5%, p=0.015; respectively). Overall survival rates were similar between the two groups after PSM (5-year survival rates: 65.4% versus 61.5% in the PG and TG groups, respectively; p = 0.54). The rate of carcinoma of remnant stomach after PG was 3.5% in this group of patients.

Conclusions

TG should be more aggressively recommended for the similar nutritional status, significantly lower reflux esophagitis and anastomotic stenosis, and free of carcinoma of remnant stomach compared with PG.
Hsa-miR-133a-3p, miR-1-3p, GOLPH3 and JUP combination results in a good biomarker to distinguish between prostate cancer and non-prostate cancer patients
Authors: Duca Rocío Belén , Massillo Cintia , Farré Paula Lucía , Graña Karen Daniela , Moro Juana , Gardner Kevin , Lacunza Ezequiel , De Siervi Adriana
Published: October 2022

The incidence and mortality of Prostate Cancer (PCa) worldwide correlate with age and bad dietary habits. Previously, we investigated the mRNA/miRNA role on PCa development and progression using high fat diet (HFD) fed mice. Here our main goal was to investigate the effect of HFD on the expression of PCa-related miRNAs and their relevance in PCa patients. We identified 6 up- and 18 down-regulated miRNAs in TRAMP-C1 mice prostate tumors under HFD conditions using miRNA microarrays. Three down-regulated miRNAs: mmu-miR-133a-3p, -1a-3p and -29c-3p were validated in TRAMP-C1 mice prostate tumor by stem-loop RT-qPCR. Hsa-miR-133a-3p/1-3p expression levels were significantly decreased in PCa compared to normal tissues while hsa-miR-133a-3p was found to be further decreased in metastatic prostate cancer tumors compared to non-metastatic PCa. We examined the promoter region of hsa-miR-133a-3p/1-3p genes and compared methylation at these loci with mature miRNA expression. We found that hsa-miR-1-2/miR-133a-1 cluster promoter hypermethylation decreased hsa-miR-133a-3p/1-3p expression in PCa. GOLPH3 and JUP, two hsa-miR-133a-3p and miR-1-3p predicted target genes, were up-regulated in PCa. ROC analysis showed that the combination of hsa-miR-133a-3p, miR-1-3p, GOLPH3 and JUP is a promising panel biomarker to distinguish between PCa and normal adjacent tissue (NAT). These results link PCa aggressiveness to the attenuation of hsa-miR-133a-3p and miR-1-3p expression by promoter hypermethylation. Hsa-miR-133a-3p and miR-1-3p down-regulation may enhance PCa aggressiveness in part by targeting GOLPH3 and JUP.
The regulation of T helper cell polarization by the diterpenoid fraction of Rhododendron molle based on the JAK/STAT signaling pathway
Authors: Qin JiaXu , Zheng XiangWei , He YanChen , Hong Yang , Liang Shuang , Fang Xin
Published: October 2022

The diterpenoid fraction (DF) prepared from fruit of Rhododendron molle was shown to have potential therapeutic effects on collagen-induced arthritis (CIA) rats based on our previous studies. As a continuation of those studies, herein, a lipopolysaccharide-induced endotoxin shock mouse model was used. The results showed that 0.2 mg/ml of DF significantly increased the mouse survival rate and had an anti-inflammatory effect. Further studies showed that DF could decrease the proportion of T helper cells (Th1 and Th17), and increase the proportion of Th2 and regulatory T cells (Tregs). Enzyme-linked immunosorbent assays indicated that DF inhibited the secretion of inflammatory cytokines such as TNF-α, IL-1β, and IL-6; western blotting showed that DF significantly reduced the levels of phosphorylated STAT1 and STAT3. In vitro, DF could dose-dependently inhibit the polarization of naive CD4+ T cells to Th1 or Th17 cells. DF at 10 μg/ml could markedly decrease the expression of mRNA encoding IFN-γ and T-bet, and suppress Th1 differentiation by downregulation of the activity of STAT1 and STAT4. Meanwhile, DF at 10 μg/ml remarkably reduced the expression of mRNA encoding IL-17a, IL-17f, and RORγt, and downregulated STAT3 phosphorylation, suggesting that DF could inhibit Th17 differentiation by reducing STAT3 activation. Taken together, DF blocked the JAK/STAT signaling pathway by inhibiting STAT1 and STAT3 phosphorylation, which clarified the important role of JAK/STAT signaling pathway in anti-rheumatoid arthritis.
Efficacy of Xinbao pill on chronic heart failure: Study protocol of a multicenter, randomized, double-blind, placebo-controlled trial
Authors: Wang Yuanping , Li Jiahua , Yan Jiaqi , Wang Yulin , Cheng Yuanyuan , Liu Zhongqiu , Wang Dawei
Published: October 2022

Introduction: Chronic heart failure (CHF) is a common cardiovascular disease. In China, Xinbao pill (XBP) is widely used as an adjuvant therapy for CHF. However, there is still a lack of high-quality clinical evidence. We designed this multicenter, randomized, double-blind, placebo-controlled trial to critically evaluate the efficacy and safety of XBP as an adjuvant treatment for patients with CHF.

Methods and analysis: We will recruit 284 patients with a clinical diagnosis of “heart-kidney yang deficiency syndrome” CHF receiving treatment in six hospitals in China. Patients will be randomly assigned, in a 1:1 ratio, to the treatment or control group using a central randomization system. All patients will receive conventional drug therapy for heart failure combined XBP (Guangdong Xinbao Pharmaceutical Co., Ltd., Guangdong, China) or a placebo. Study physicians, subjects, outcome assessors, and statisticians will be blinded to the group assignment. The primary outcome will be the change in the proportion of patients who show a decrease in serum NT-proBNP of more than 30% after treatment. Secondary outcomes are NYHA class, 6-minute walk distance test, Minnesota Quality of Life Scale score, endpoint events, serum NT-proBNP, echocardiographic parameters, and traditional Chinese medicine (TCM) symptom score. Adverse events will be monitored throughout the trial. Data will be analyzed according to a predetermined statistical analysis plan.

Discussion: The results of this study will provide solid evidence of the safety and efficacy of XBP as an alternative and complementary treatment measure for patients with CHF.

Clinical Trial Registration: Chinese Clinical Trial Registration Center (ChiCTR2000038492).
A comparative study of four types of multi-scale entropies in feature extraction of underwater acoustic signals for potential GNSS positioning applications
Authors: Zhao Danning , Lei Yu , Xu Jinsong , Cai Hongbing
Published: October 2022

The combination of underwater acoustic processing and the Global Navigation Satellite System (GNSS) has achieved remarkable economic benefits in offshore operations. As the key technology of GNNS positioning, feature extraction of underwater acoustic signals is affected by the complex marine environment. To extract more effective information from underwater acoustic signals, we use four types of multi-scale entropies, including multi-scale sample entropy (MSE), multi-scale fuzzy entropy (MFE), multi-scale permutation entropy (MPE), and multi-scale dispersion entropy (MDE), to analyze and distinguish underwater acoustic signals. In this study, two groups of real-word underwater acoustic signal experiments were performed for feature extraction of ship-radiated noises (SRNs) and ambient noises (ANs). The results indicated that the performance of the MFE-based feature extraction method is superior to that of feature extraction methods based on the other three entropies under the same number of features, and the highest average recognition rate (ARR) of the MFE-based feature extraction method for SRNs reaches 100% when the number of features is 3.
A weight optimization-based transfer learning approach for plant disease detection of New Zealand vegetables
Authors: Saleem Muhammad Hammad , Potgieter Johan , Arif Khalid Mahmood
Published: October 2022

Deep learning (DL) is an effective approach to identifying plant diseases. Among several DL-based techniques, transfer learning (TL) produces significant results in terms of improved accuracy. However, the usefulness of TL has not yet been explored using weights optimized from agricultural datasets. Furthermore, the detection of plant diseases in different organs of various vegetables has not yet been performed using a trained/optimized DL model. Moreover, the presence/detection of multiple diseases in vegetable organs has not yet been investigated. To address these research gaps, a new dataset named NZDLPlantDisease-v2 has been collected for New Zealand vegetables. The dataset includes 28 healthy and defective organs of beans, broccoli, cabbage, cauliflower, kumara, peas, potato, and tomato. This paper presents a transfer learning method that optimizes weights obtained through agricultural datasets for better outcomes in plant disease identification. First, several DL architectures are compared to obtain the best-suited model, and then, data augmentation techniques are applied. The Faster Region-based Convolutional Neural Network (RCNN) Inception ResNet-v2 attained the highest mean average precision (mAP) compared to the other DL models including different versions of Faster RCNN, Single-Shot Multibox Detector (SSD), Region-based Fully Convolutional Networks (RFCN), RetinaNet, and EfficientDet. Next, weight optimization is performed on datasets including PlantVillage, NZDLPlantDisease-v1, and DeepWeeds using image resizers, interpolators, initializers, batch normalization, and DL optimizers. Updated/optimized weights are then used to retrain the Faster RCNN Inception ResNet-v2 model on the proposed dataset. Finally, the results are compared with the model trained/optimized using a large dataset, such as Common Objects in Context (COCO). The final mAP improves by 9.25% and is found to be 91.33%. Moreover, the robustness of the methodology is demonstrated by testing the final model on an external dataset and using the stratified k-fold cross-validation method.
RNA-Seq combined with population-level analysis reveals important candidate genes related to seed size in flax (Linum usitatissimum L.)
Authors: Jiang Haixia , Guo Dongliang , Liu Yuanyuan , Zhu Leilei , Xie Fang , Xie Liqiong
Published: October 2022

Seed size is a key determinant of crop yields. Understanding the regulatory mechanisms of seed size is beneficial for improving flax seed yield. In this study, the development of large flax seeds lagged behind that of small seeds, and 1,751 protein-coding genes were differentially expressed in early seeds, torpedo-stage embryos, and endosperms of CIli2719 and Z11637 using RNA sequencing. Homologous alignment revealed that 129 differentially expressed genes (DEGs) in flax were homologous with 71 known seed size–related genes in Arabidopsis thaliana and rice (Oryza sativa L.). These DEGs controlled seed size through multiple processes and factors, among which phytohormone pathways and transcription factors were the most important. Moreover, 54 DEGs were found to be associated with seed size and weight in a DEG-based association study. Nucleotide diversity (π) analysis of seed size–related candidate DEGs by homologous alignment and association analysis showed that the π values decreased significantly during flax acclimation from oil to fiber flax, suggesting that some seed size–related candidate genes were selected in this acclimation process. These results provide important resources and genetic foundation for further research on seed size regulation and seed improvement in flax.
Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
Authors: Paterlini A. , Sechet J. , Immel F. , Grison M. S. , Pilard S. , Pelloux J. , Mouille G. , Bayer E. M. , Voxeur A.
Published: October 2022

Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thaliana cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Full rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD.
Characterization of a pathway−specific activator of edeine biosynthesis and improved edeine production by its overexpression in Brevibacillus brevis
Authors: Du Jie , Zhang Cuiyang , Long Qingshan , Zhang Liang , Chen Wu , Liu Qingshu
Published: October 2022

Edeines are a group of non-ribosomal antibacterial peptides produced by Brevibacillus brevis. Due to the significant antibacterial properties of edeines, increasing edeine yield is of great interest in biomedical research. Herein, we identified that EdeB, a member of the ParB protein family, significantly improved edeine production in B. brevis. First, overexpression of edeB in B. brevis X23 increased edeine production by 92.27%. Second, in vitro bacteriostasis experiment showed that edeB-deletion mutant exhibited less antibacterial activity. Third, RT-qPCR assay demonstrated that the expression of edeA, edeQ, and edeK, which are key components of the edeine biosynthesis pathway, in edeB-deletion mutant X23(ΔedeB) was significantly lower than that in wild-type B. brevis strain X23. Finally, electrophoretic mobility shift assay (EMSA) showed that EdeB directly bound to the promoter region of the edeine biosynthetic gene cluster (ede BGC), suggesting that EdeB improves edeine production through interaction with ede BGC in B. brevis.
The upstream regulatory mechanism of BplMYB46 and the function of upstream regulatory factors that mediate resistance to stress in Betula platyphylla
Authors: Guo Huiyan , Sun Xiaomeng , Wang Bo , Wu Di , Sun Hu , Wang Yucheng
Published: October 2022

Previously, we have shown that the transcription factor BplMYB46 in Betula platyphylla can enhance tolerance to salt and osmotic stress and promote secondary cell wall deposition, and we characterized its downstream regulatory mechanism. However, its upstream regulatory mechanism remains unclear. Here, the promoter activity and upstream regulatory factors of BplMYB46 were studied. Analyses of β-glucuronidase (GUS) staining and activity indicated that BplMYB46 promoter was specific temporal and spatial expression, and its expression can be induced by salt and osmotic stress. We identified three upstream regulatory factors of BplMYB46: BpDof1, BpWRKY3, and BpbZIP3. Yeast-one hybrid assays, GUS activity, chromatin immunoprecipitation, and quantitative real-time polymerase chain reaction revealed that BpDof1, BpWRKY3, and BpbZIP3 can directly regulate the expression of BplMYB46 by specifically binding to Dof, W-box, and ABRE elements in the BplMYB46 promoter, respectively. BpDof1, BpWRKY3, and BpbZIP3 were all localized to the nucleus, and their expressions can be induced by stress. Overexpression of BpDof1, BpWRKY3, and BpbZIP3 conferred the resistance of transgenic birch plants to salt and osmotic stress. Our findings provide new insights into the upstream regulatory mechanism of BplMYB46 and reveal new upstream regulatory genes that mediate resistance to adverse environments. The genes identified in our study provide novel targets for the breeding of forest tree species.
The effects of wakeful rest on memory consolidation in an online memory study
Authors: King Olivia , Nicosia Jessica
Published: October 2022

Memory consolidation is the process in which memory traces are strengthened over time for later retrieval. Although some theories hold that consolidation can only occur during sleep, accumulating evidence suggests that brief periods of wakeful rest may also facilitate consolidation. Interestingly, however, Varma and colleagues reported that a demanding 2-back task following encoding produced a similar performance to a wakeful reset condition. We tested whether participants’ recall would be best following a wakeful rest condition as compared to other distractor conditions, consistent with the extant wakeful rest literature, or whether we would replicate the finding by Varma and colleagues such that participants’ memory benefitted from both a rest and a 2-back task following encoding. Across two experiments, we used similar (Experiment 1) and the same (Experiment 2) encoding material as used the one by Varma and colleagues, employed a wakeful rest condition adapted for online testing, and compared participants’ recall across post-encoding conditions. In the first experiment, we used a between-subjects design and compared participants’ cued recall performance following a period of wakeful rest, a 2-back task, or a rest + sounds condition. The second experiment more closely replicated the experimental design used by Varma and colleagues using a within-subjects manipulation. Ultimately, our findings more consistently aligned with the canonical wakeful rest finding, such that recall was better following the rest condition than all other post-encoding conditions. These results support the notion that wakeful rest may allow for consolidation by protecting recently encoded information from interference, thereby improving memory performance.
Green human resources management: A novel tool to boost work engagement
Authors: Baykal Elif , Bayraktar Osman
Published: October 2022

Increasing environmental awareness in business life has given way to Green Human Resources Management practices. The positive corporate image created by GHRM is encouraging for many employees and boosts their work engagement. GHRM practices make employees feel proud about their organization and creates a value-based ground for working in their current companies. Actually, internalized green inclinations of organizations, namely, value alignment between an organization and an employee, can make their employees feel greater psychological ownership about their companies, leading to greater engagement as it is suggested in social identity theory. Being inspired from psychological ownership literature, in this study, it is assumed that being exposed to Green Human Resources Management practices can make employees feel higher levels of work engagement and psychological ownership can have a mediator effect in this relationship. The model has been a tested model among 255 Turkish white-collar employees working in a manufacturing sector. Analyses have been carried out using the AMOS structural equation program and the PROCESS program. Results confirmed the above assumptions, confirming the assumptions of social identity theory and revealed the existence of mediator effect in the relationship between GHRM and work engagement of employees, illuminating the importance of GHRM for employees' positive attitudes toward their organization.
Effects of country animosity of angry Koreans on Japan: A focus on export regulation on Korea
Authors: Sun Lili , Jun Jong-Woo
Published: October 2022

Nowadays, Korea and Japan are in conflict arising from export restrictions launched by Japan on Korea, which have provoked a boycott of Japanese products in Korea, and even tourism to Japan. Animosity performs a momentous role in the context of crisis management communication. Hence, this article aims to investigate factors impacting boycott intention to visit Japan, with economic animosity being a principal mediating variable, whose antecedents and consequences have been probed into. A total of 333 respondents' survey data were collected and analyzedviaSEM for the verification of research hypotheses. The findings manifest that ethnic identity engenders significant direct positive bearings upon economic animosity and boycott news, and boycott news significantly positively affects economic animosity; boycott news serves as the mediating role between ethnic identity and economic animosity. Additionally, the outcomes denote that economic animosity exerts a significant positive impact on boycott visit intention, economic animosity negatively affects Japanese government trust, and Japanese government trust negatively bears upon boycott visit intention; Japanese government trust mediates between economic animosity and boycott intention to visit Japan. Consequently, the research makes contributions to furnishing empirical evidence for influencing factors of boycott visit intention and enriching the literature on the antecedents and consequences of animosity.
Characteristics of interbody bone graft fusion after transforaminal lumbar interbody fusion according to intervertebral space division
Authors: Xu Songjie , Zang Lei , Lu Qian , Zhao Peng , Wu Qichao , Chen Xueming
Published: October 2022

Background

According to intervertebral space division, the characteristics of interbody bone graft fusion after transforaminal lumbar interbody fusion (TLIF) were assessed via computed tomography (CT) scan to provide a theoretical basis for selecting the bone grafting site of interbody fusion.

Methods

The medical records of 57 patients with lumbar spinal stenosis and disc herniation treated with TLIF were analysed retrospectively. In total, 57 segments received lumbar interbody fusion. A thin-layer CT scan was performed to evaluate fusion in each zone of the fusion space.

Results

The fusion rates were 57.89% (n = 33) in the anterior cage zone, 73.68% (n = 42) in the posterior cage zone, 66.67% (n = 38) in the decompression zone, 26.32% (n = 15) in the contralateral decompression zone and 94.74% (n = 54) in the inner cage zone. There were significant differences among the fusion rates of the five zones (P < 0.001). Further pairwise comparison revealed that the fusion rates in the inner cage significantly differed from the anterior and posterior cages and decompression and contralateral decompression zones (P = 0.001, 0.002, 0.001 and 0.001, respectively).

Conclusion

We think the central cage zone (i.e., inner cage) should be the focus of bone grafting. Although there is small volume of bone graft on the posterior cage zone, the fusion rate is relatively high, only secondary to the inner cage zone. The fusion rate is of the contralateral decompression zone is lower although there is a bone graft.
Survival benefit of surgical resection for stage IV gastric cancer: A SEER-based propensity score-matched analysis
Authors: Sun Jianhui , Nan Qiong
Published: October 2022

Background

Gastric cancer (GC) is a major malignancy worldwide, and its incidence and mortality rate are increasing year by year. Clinical guidelines mainly use palliative drug combination therapy for stage IV gastric cancer. In accordance with some small sample studies, surgery can prolong survival. There is no uniform treatment plan for stage IV gastric cancer. This study focused on collecting evidence of the survival benefit of cancer-directed surgery (CDS) for patients with stage IV gastric cancer by analyzing data from a large sample.

Methods

Data on patients with stage IV gastric cancer diagnosed between 2010 and 2015 was extracted and divided into CDS and no-CDS groups using the large dataset in the Surveillance, Epidemiology, and End Results (SEER) database. With bias between the two groups minimized by propensity score matching (PSM), the prognostic role of CDS was studied by the Cox proportional risk model and Kaplan-Meier.

Results

A total of 6,284 patients with stage IV gastric cancer were included, including 514 patients with CDS who were matched with no-CDS patients according to propensity score (1:1), resulting in the inclusion of 432 patients each in the CDS and no-CDS groups. The results showed that CDS appeared to prolong the median survival time for stage IV gastric cancer (from 6 months to 10 months). Multifactorial analysis showed that poorly differentiated tumors (grades III-IV) significantly affected patient survival, and chemotherapy was a protective prognostic factor.

Conclusion

The findings support that CDS can provide a survival benefit for stage IV gastric cancer. However, a combination of age, underlying physical status, tumor histology, and metastatic status should be considered when making decisions about CDS, which will aid in clinical decision-making.
The turbocharged wide anterolateral thigh perforator flap to reconstruct massive soft tissue defects in traumatized lower extremities: A case series
Authors: Jeong Seong-Ho , Koo Do-Yoon , Moon Kyung-Chul , Dhong Eun-Sang , Han Seung-Kyu
Published: October 2022

Background

Extensive traumatic soft tissue defects in the lower extremities typically require complete coverage of exposed bone because inadequate coverage, such as partial flap loss, may result in bony infection and ultimately lead to limb salvage failure. To achieve complete coverage of these defects, we used the wide anterolateral thigh perforator flap in which the turbocharging procedure augments the blood flow. Herein, we describe our turbocharging technique and discuss its effectiveness.

Methods

From January 2014 to December 2020, the turbocharged wide ALTP free flaps were used to treat 13 patients with massive traumatic soft tissue defects in the lower extremities, ranging in size from 22 × 10 cm2 (220 cm2) to 21 × 17 cm2 (357 cm2) (mean, 270 cm2). All ALTP flaps were supplied by perforators from both the transverse branch of the lateral circumflex artery (TB-LCFA) and descending branch of the lateral circumflex artery (DB-LCFA) simultaneously. The turbocharging procedure by connecting the TB-LCFA to a side branch of the DB-LCFA was carried out in all these flaps. A retrospective review of medical records for each patient was performed.

Results

The size of the transferred ALTP flap ranged from 23 × 12 cm2 (276 cm2) to 23 × 19 (437 cm2) (mean, 331 cm2). The total number of perforators included in the flaps was three on average. All ALTP flaps survived completely without partial necrosis. The postoperative course was uneventful except for two cases with minor complications, including hematoma and partial necrosis of the recipient's skin.

Conclusion

Free transfer of the turbocharged wide ALTP flap can be a reliable and effective reconstructive method to obtain complete coverage of extensive traumatic soft tissue defects in the lower extremities and achieve successful limb salvage.
Th1, Th2, and Th17 cells and their corresponding cytokines are associated with anxiety, depression, and cognitive impairment in elderly gastric cancer patients
Authors: Zhou Yanxia , Yu Ke
Published: October 2022

Objective

T helper (Th) cells modulate the stress response, oxidative stress, and neuroinflammation to mediate anxiety, depression, and cognitive impairment. This study intended to explore the association between Th cells and anxiety, depression, and cognitive impairment in elderly gastric cancer patients.

Methods

Totally, 176 elderly gastric cancer patients were enrolled in this study. Peripheral blood samples were collected. Th1, Th2, and Th17 cells were detected by flow cytometry; their corresponding cytokines were examined by ELISA. The Hospital Anxiety and Depression Scale (HADS) and Mini-Mental State Examination (MMSE) were assessed.

Results

In total, 42.0%, 33.0%, and 19.9% of elderly gastric cancer patients presented anxiety, depression, and cognitive impairment, respectively. Th1 (P = 0.016), Th17 (P = 0.009), and IL-17A (P = 0.001) were positively associated with the HADS-A score. Th17 (P = 0.003) and IL-17A (P = 0.009) levels were increased in patients with anxiety compared with those without anxiety. Concurrently, a positive association was observed for Th1 (P = 0.027), Th17 (P = 0.014), and IFN-γ (P = 0.049) with the HADS-D score. Th1 (P = 0.017) and Th17 (P = 0.049) levels were increased in patients with depression than in those without depression. Moreover, Th1 (P = 0.003), Th17 (P < 0.001), IFN-γ (P = 0.014), and IL-17A (P < 0.001) were inversely related to MMSE scores, but only Th17 (P < 0.001) and IL-17A (P < 0.001) were increased in patients with cognitive impairment compared with those without cognitive impairment.

Conclusion

Th1 and Th17 cells reflect anxiety, depression, and cognitive impairment risk to a certain extent in elderly gastric cancer patients, implying their involvement in the pathology of the abovementioned psychological and cognitive issues. However, further validation is needed.
Potential Global Distribution of Invasive Alien Species, Anthonomus grandis Boheman, under Current and Future Climate Using Optimal MaxEnt Model
Authors: Jin Zhenan , Yu Wentao , Zhao Haoxiang , Xian Xiaoqing , Jing Kaiting , Yang Nianwan , Lu Xinmin , Liu Wanxue
Published: October 2022

The boll weevil, Anthonomus grandis Boheman (Coleoptera: Curculionidae), is an invasive alien species that can damage cotton plants and cause huge economic losses in the cotton industry. Currently, A. grandis is mainly distributed in the American continent. However, few studies have indicated the distribution and modification of its suitable global habitats after undergoing climate change. Based on the 339 distribution records of A. grandis and eight bioclimatic variables, we used the optimal MaxEnt model to predict the potential global distribution of A. grandis under the current (1970–2000) and future climatic scenarios (SSP5-8.5). The annual mean temperature (bio1) and isothermality (bio3) were the two most important bioclimatic variables, which indicates that the survival of A. grandis is extremely sensitive to temperature fluctuations. Under the current scenario, the highly suitable habitats were mainly distributed in America (the USA, Mexico, Brazil, Argentina, Paraguay, and Uruguay), Africa (South Africa, Ethiopia, and Mozambique), Asia (Pakistan, India, Thailand, Burma, and China), and Oceania (Australia). In future scenarios (SSP5-8.5), the potential suitable global habitats reached the highest level in America, Africa, Asia, and Oceania in the 2090s. Our study provides a meaningful reference for researchers, quarantine officers, and governments to devise suitable management control strategies for A. grandis.
The Impact of Government Agricultural Development Support on Agricultural Income, Production and Food Security of Beneficiary Small-Scale Farmers in South Africa
Authors: Mokgomo Mahlako Nthabeleng , Chagwiza Clarietta , Tshilowa Phathutshedzo Fancy
Published: October 2022

Enormous Literature indicates that agriculture remains a source of livelihood for about 86% of rural people and generates job opportunities for approximately 1.3 billion small-scale farmers and landless workers. Over the past couple of years, the South African government has been offering varied support to households that are engaged in small-scale farming to improve their livelihoods, income and food security. Although the various rounds of the General Households Survey (GHS) gathered information on the type of agricultural support received by the farmers about their food production, agricultural income and food security status, there is still limited pragmatic evidence on the extent to which programme is yielding the intended results. The main aim of the study was to use GHS data spanning the period 2013 to 2016 to assess how government agricultural development support influences the livelihoods of small-scale farmers in South Africa. Using both descriptive analyses with Propensity Score Matching (PSM) and Logistics estimations, the result of the study indicates that the proportion of households who have access to the agricultural development support have decreased marginally by two percent from 16% in 2013 to 14% in 2016. The study also reveals that agriculture development assistance given by the South African government is effective in reducing food insecurity, improving agricultural production and income of the beneficiary small-scale farmers. Following the observed marked gender, racial and geographical differences in households’ access to the agricultural development support, the Ministry of Agriculture and its allied ministries and departments responsible for the implementation of the agricultural development support programmes must streamline policies to account for the lack of support to farmers in general. Addressing such differences is necessary to ensure that the programme achieves its intended overall objectives.
The Vigor of Seed Potatoes from Organic and Conventional Systems
Authors: Zarzyńska Krystyna , Boguszewska-Mańkowska Dominika , Feledyn-Szewczyk Beata , Jończyk Krzysztof
Published: October 2022

In the research carried out in 2018–2020 at the Plant Breeding and Acclimatization Institute, the vigor of seed potatoes from two production systems—organic and conventional—was examined. The characteristics of the tubers and the plants grown from them were assessed. It was found that seed potatoes from the organic system were characterized by features showing symptoms of weaker vigor, i.e., a shorter resting period, a smaller percentage of germinating eyes, a smaller mass of sprouts produced, and a shorter length of the longest sprout. The weaker vigor of seed potatoes from the organic system was reflected in the development and yield of the plants derived from them. It was expressed in weaker emergence, lower stem number, and smaller yield per plot. However, there were no differences in the yield structure. A large varietal differentiation was demonstrated for most of the studied traits. The differences in the vigor of individual cultivars were not related to the length of the growing season. The conditions prevailing in the research years had the greatest impact on the length of tuber dormancy, the percentage of germinating eyes, and the percentage of plant emergence.
Comprehensive Evaluation of Morpho-Physiological and Ionic Traits in Wheat (Triticum aestivum L.) Genotypes under Salinity Stress
Authors: Chaurasia Shiksha , Kumar Arvind , Singh Amit Kumar
Published: October 2022

Salinity is the foremost abiotic stress that severely affects plant growth and constrains its productivity worldwide. In the present investigation, genetic variation in wheat genotypes was evaluated to identify novel salt-tolerant genetic resources, which could be used in the bread wheat improvement program. A diverse panel of 44 different wheat genotypes was evaluated at seedling stage to characterize morphological and ionic traits under salt stress (150 mM NaCl). Salt treatment caused 33.33, 45.31, 55.17, and 72.53% reduction in root dry weight (RDW), root fresh weight (RFW), shoot dry weight (SDW), and shoot fresh weight (SFW), respectively. Under salt stress, maximum inhibition of Na+ ion uptake was observed in tolerant genotypes, and this was accompanied by a high Ca2+ uptake. Wheat genotypes showed a wide spectrum of responses under salt stress; however, four genotypes, EC576356, IC533596, IC279230, and IC290188, exhibited consistent performance, which was strongly linked to proper Na+ and K+ discrimination in leaves. The tolerant genotypes acquired a better ability to maintain stable relative water content (RWC), chlorophyll (CHL), and photosynthesis rate (PS), resulting in significantly higher dry matter production under salt stress. Further, biomass, shoot K+, root Ca2+, and shoot K+/Na+ were identified as the most effective parameters for screening wheat germplasm for salinity tolerance. The identified germplasm could be used as donors for transferring salt tolerance to improved cultivars as well as in further genetic studies to uncover the genetic mechanisms governing salt stress response in wheat.
Determinants of Changes in Fruit Production in Farms in Areas with a Fragmented Agrarian Structure
Authors: Szymańska Elżbieta Jadwiga , Rysz Maria
Published: October 2022

The main aim of the research was to identify the factors influencing the production of fruit in a region with a fragmented agrarian structure. The novelty of the article is taking into account changes over time. The importance of the research topics stems from the fact that Poland is a significant producer of many species of fruit. Part of this production is carried out on small farms. A panel model with fixed effects (FE) was used to identify the factors. The data for the model came from 18 farms from the Małopolska and Pogórze Region in Poland, which continuously, from 2005–2016, kept accounting under Farm Accountancy Data Network (FADN). The combination of cross-sectional data from 18 farms and a 12-year time series increased the dataset to 216 observations that formed the panel; consequently, the same research period for all farms allowed for the construction of a balanced panel. The research shows that the amount of fruit production in the researched orchard farms was influenced by the value of current assets, the cost of salaries and the age of fruit growers with which experience in running orchards was related.
Allelopathic Effects of Foliar Epichloë Endophytes on Belowground Arbuscular Mycorrhizal Fungi: A Meta-Analysis
Authors: Zhong Rui , Zhang Lin , Zhang Xingxu
Published: October 2022

Many grasses are simultaneously symbiotic with Epichloë fungal endophytes and arbuscular mycorrhizal fungi (AMF). Epichloë endophytes are a group of filamentous fungi that colonize and grow within aerial plant tissues, such as leaves and stems. Infection and hyphal growth of Epichloë endophytes confer fitness advantages to the host plants. In addition to producing fungal alkaloids and altering host metabolic/genetic profiles, it is proven that symbiosis of plants with root/foliar endophytes affects the plant–soil relationship. We propose that the Epichloë presence/infection results in variations of soil and root AMF through allelopathic effects. We performed a meta-analysis that integrated the allelopathic effects of Epichloë endophytes on grass–AMF development. In the pre-symbiotic phase of grass–AMF symbiosis, root exudation from Epichloë-infected plants positively affected AMF growth, whereas the shoot exudates of Epichloë-infected plants inhibited AMF growth. In the symbiotic phase of grass–AMF symbiosis, the Epichloë infection was found to reduce root mycorrhizal colonization in plants. No pattern in the response of soil AMF to Epichloë presence was found. This study should improve our understanding of the impact of Epichloë endophytes on belowground microbial symbionts within the same host plant. Grass–Epichloë–AMF symbiosis may become an important model for studying above–belowground interactions.