bluuebunny/crossref_metadata_2025 huggingface dataset:
Lithological and Geochemical Heterogeneity of the Organo-Mineral Matrix in Carbonate-Rich Shales
Authors: Bogdanovich Natalia , Kozlova Elena , Karamov Tagir
Published: July 2021

The paper discusses the issues of interaction of the organic matter and the siliceous-carbonate mineral matrix in unconventional reservoirs of the Upper Devonian Domanik Formation of the Upper Kama Depression of the Volga-Ural Basin. The Domanik Formation is composed of organic-rich low-permeability rocks. Lithological and geochemical peculiarities of rocks were studied using light microscopy, X-ray diffraction analysis (XRD), scanning electronic microscopy (SEM), and evaporation method. Organic matter was examined by the Rock-Eval pyrolysis with quantitative and qualitative evaluation of generation potential and maturity degree. Integrated analysis of results of lithological and geochemical studies allowed identifying intervals in the studied section where organic matter can form a complex association with the siliceous-carbonate matrix. It was fixed experimentally that in some cases the mineral carbonate matrix and the organic matter form a one-whole high-molecular compound. The authors supposed that in the course of sedimentation, organic matter is immobilized into the structure of the mineral carbonate matrix. At the deposition and diagenesis stage, the carbonate matter interacts with acids of the organic matter and forms natural organo-mineral polymers. Special physicochemical properties of such organo-mineral associations shed new light onto the problems of producing from hard-to-develop nonconventional carbonate reservoirs and evaluating the associated risks.
Tumor Characteristic Variations between Symptomatic and Asymptomatic Endometrial Cancer
Authors: Vinklerová Petra , Bednaříková Markéta , Minář Luboš , Felsinger Michal , Hausnerová Jitka , Ovesná Petra , Weinberger Vít
Published: July 2021

Endometrial cancer is the most common gynecologic malignancy in Europe and usually diagnosed in its initial stage owing to early symptoms of abnormal bleeding. There is no population screening for this disease, although it can sometimes be accidentally diagnosed in asymptomatic patients. Our study aims to determine differences in clinical and tumor characteristics between an asymptomatic and symptomatic group of patients. This unicentric prospective observational study took place in University Hospital Brno between January 2016 and December 2019. A total of 264 patients met inclusion criteria (26% asymptomatic, 74% with reported symptoms). We did not find a statistically significant difference in clinical characteristics (menopausal status, parity, age, BMI, and serum level of CA 125) between groups. According to ultrasound examination, bleeding tumors were larger (19.5 vs. 12.7 mm, p ≤ 0.001). Definitive histology results indicated more frequent lymphovascular space invasion (p < 0.001), along with deep myometrial (p = 0.001) and cervical (p = 0.002) invasion. There was no difference in advanced stages of the tumor. We did not substantiate statistically significant difference in immunohistochemical profile (estrogen and progesterone receptors, L1 cell adhesion molecule, tumor protein p53), which is relevant for tumor recurrence risk and survival capacity. Our conclusions affirmed that bleeding occurs more often among patients with local tumor invasion into the myometrium and cervical stroma. Final International Federation of Gynecology and Obstetrics (FIGO) stage, histology, and immunohistochemical characteristics do not significantly affect symptom appearance.
Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo
Authors: Shulgina Alla A. , Kalashnikova Elena A. , Tarakanov Ivan G. , Kirakosyan Rima N. , Cherednichenko Mikhail Yu. , Polivanova Oksana B. , Baranova Ekaterina N. , Khaliluev Marat R.
Published: July 2021

We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on the morphophysiological parameters of Stevia rebaudiana Bertoni in vitro and in vivo. Both PGRs and the light spectra applied were found to significantly affect plant morphogenesis. During the micropropagation stage of S. rebaudiana, optimal growth, with a multiplication coefficient of 15, was obtained in an MS culture medium containing 2,4-epibrassinolide (Epin) and indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L−1, respectively. During the rooting stage, we found that the addition of 0.5 mg L−1 hydroxycinnamic acid (Zircon) to the MS medium led to an optimal root formation frequency of 85% and resulted in the formation of strong plants with well-developed leaf blades. Cultivation on media containing 0.1 mg L−1 Epin and 0.5 mg L−1 IAA and receiving coherent light irradiation on a weekly basis resulted in a 100% increase in the multiplication coefficient, better adventitious shoot growth, and a 33% increase in the number of leaves. S. rebaudiana microshoots, cultured on MS media containing 1.0 mg L−1 6-benzylaminopurine (BAP) and 0.5 mg L−1 IAA with red monochrome light treatments, increased the multiplication coefficient by 30% compared with controls (white light, media without PGRs).
Review of Associations between Built Environment Characteristics and Severe Acute Respiratory Syndrome Coronavirus 2 Infection Risk
Authors: Wang Jingjing , Wu Xueying , Wang Ruoyu , He Dongsheng , Li Dongying , Yang Linchuan , Yang Yiyang , Lu Yi
Published: July 2021

The coronavirus disease 2019 pandemic has stimulated intensive research interest in its transmission pathways and infection factors, e.g., socioeconomic and demographic characteristics, climatology, baseline health conditions or pre-existing diseases, and government policies. Meanwhile, some empirical studies suggested that built environment attributes may be associated with the transmission mechanism and infection risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, no review has been conducted to explore the effect of built environment characteristics on the infection risk. This research gap prevents government officials and urban planners from creating effective urban design guidelines to contain SARS-CoV-2 infections and face future pandemic challenges. This review summarizes evidence from 25 empirical studies and provides an overview of the effect of built environment on SARS-CoV-2 infection risk. Virus infection risk was positively associated with the density of commercial facilities, roads, and schools and with public transit accessibility, whereas it was negatively associated with the availability of green spaces. This review recommends several directions for future studies, namely using longitudinal research design and individual-level data, considering multilevel factors and extending to diversified geographic areas.
Relationship between Built Environment and COVID-19 Dispersal Based on Age Stratification: A Case Study of Wuhan
Authors: Niu Qiang , Wu Wanxian , Shen Jie , Huang Jiaxin , Zhou Qiling
Published: July 2021

The outbreak of COVID-19 (coronavirus disease 2019) has become the focus of attention in the field of urban geography. Built environment, such as the layout of public spaces like transportation hubs and urban open spaces, is an important factor affecting the spread of the epidemic. However, due to the different behavior patterns of different age groups, the intensity and frequency of their use of various built environment spaces may vary. Based on this, we selected patients that were infected, with a non-manipulated time period, and the classification of human behavior patterns; we then conducted a regression analysis study on the spatial distribution and building environment of these COVID-19 patients. The results showed that the spatial distribution of young and middle-aged patients (18–59 years old) was more homogeneous, while the spatial distribution of elderly patients (60 years old and above) had a strong clustering characteristic. Moreover, the significant built environment factors exhibited in the two populations were extremely different. More diverse urban facilities and public spaces exhibited influential properties for older patients, while middle-aged and young adults were more influenced by commuting facilities. It can be said that the built environment shows different influences and mechanisms on the transmission of respiratory infectious diseases in different populations. Therefore, the results of this paper can inform decision makers who expect to reduce the occurrence of urban respiratory infectious diseases by improving the urban built environment.
Teacher Teleworking during the COVID-19 Pandemic: Association between Work Hours, Work–Family Balance and Quality of Life
Authors: Lizana Pablo A. , Vega-Fernadez Gustavo
Published: July 2021

Background: Teachers worldwide had to reinvent their work routine according to teleworking during the COVID-19 pandemic, a work format that negatively impacts individuals’ physical and mental health. This study evaluates the association between work hours, work–family balance and quality of life (QoL) among teachers during the Chilean health emergency of the COVID-19 pandemic. Teachers from across Chile were contacted via email and social media to answer an online survey. QoL was evaluated via the SF-36 questionnaire, work hours and work–family balance in the pandemic. A total of 336 teachers from across Chile participated in this study. Teachers had a low QoL score, associated with age (p < 0.05). Teachers who were ≤44 showed lower deterioration risks in the Physical Component Summary (OR: 0.54) than the ≥45-year-old age group; simultaneously, the younger group (≤44 years) had a greater risk (OR: 2.46) of deterioration in the Mental Component Summary than teachers over 45 years. A total of 78.7% of teachers reported having increased their work hours during the COVID-19 pandemic due to teleworking and 86% indicated negative effects on their work–family balance. Pandemic work hours and negative work–family balance increase the risk of reducing the Mental Component Summary (OR: 1.902; OR: 3.996, respectively). Teachers presented low median QoL scores, especially in the Mental Component Summary, suggesting that it would be beneficial to promote a better workload distribution for teachers in emergency contexts, considering the adverse effects of teleworking.
Evaluation of a Pediatric Obesity Management Toolkit for Health Care Professionals: A Quasi-Experimental Study
Authors: Patel Barkha P. , Hadjiyannakis Stasia , Clark Laurie , Buchholz Annick , Noseworthy Rebecca , Bernard-Genest Julie , Walsh Catharine M. , McPherson Amy C. , Strub Jonah , Strom Michele , Hamilton Jill K.
Published: July 2021

Health care professionals (HCPs) play a critical role in helping to address weight-related issues with pediatric patients, yet often feel ill-equipped to discuss/manage this complex and sensitive health issue. Using the five As (“Ask, Assess, Advise, Agree, and Assist”) of Pediatric Obesity Management, we created a series of educational videos and evaluated the content, quality (acceptability, engagement), and impact of these videos on HCPs’ self-efficacy, knowledge, and change in practice when addressing weight-related issues with pediatric patients and their families using questionnaires. HCPs (n = 65) participated in a baseline assessment and 4–6 month follow-up (n = 54). Knowledge and self-efficacy increased post-video for the majority of participants. At follow-up, most HCPs reported a change in their practice attributable to viewing the videos, and their self-efficacy ratings improved over time for the majority of questions asked. Most participants rated aspects of each of the videos highly. Preliminary findings suggest that an evidence-based educational toolkit of videos, based on the 5As framework, may lead to changes in self-reported behaviors among HCPs, and sustained improvements in their self-efficacy in addressing weight-related topics with children and their families. (Clinical Trial Number NCT04126291).
The Impact of Nordic Walking on Bone Properties in Postmenopausal Women with Pre-Diabetes and Non-Alcohol Fatty Liver Disease
Authors: Du Xiaming , Zhang Chao , Zhang Xiangqi , Qi Zhen , Cheng Sulin , Le Shenglong
Published: July 2021

This study investigated the impact of Nordic walking on bone properties in postmenopausal women with pre-diabetes and non-alcohol fatty liver disease (NAFLD). A total of 63 eligible women randomly participated in the Nordic walking training (AEx, n = 33), or maintained their daily lifestyle (Con, n = 30) during intervention. Bone mineral content (BMC) and density (BMD) of whole body (WB), total femur (TF), femoral neck (FN), and lumbar spine (L2-4) were assessed by dual-energy X-ray absorptiometry. Serum osteocalcin, pentosidine, receptor activator of nuclear factor kappa-B ligand (RANKL) levels were analyzed by ELISA assay. After an 8.6-month intervention, the AEx group maintained their BMCTF, BMDTF, BMCL2−4, and BMDL2−4, and increased their BMCFN (p = 0.016), while the Con group decreased their BMCTF (p = 0.008), BMDTF (p = 0.001), and BMDL2−4 (p = 0.002). However, no significant group × time interaction was observed, except for BMDL2−4 (p = 0.013). Decreased pentosidine was correlated with increased BMCWB(r = −0.352, p = 0.019). The intervention has no significant effect on osteocalcin and RANKL. Changing of bone mass was associated with changing of pentosidine, but not with osteocalcin and RANKL. Our results suggest that Nordic walking is effective in preventing bone loss among postmenopausal women with pre-diabetes and NAFLD.
The Effectiveness of Active Rehabilitation Camp on Physical Performance of Disabled People Moving in Wheelchairs
Authors: Lipert Anna , Wróbel Kacper , Spychała Michał , Rasmus Paweł , Timler Dariusz , Marczak Michał , Kozłowski Remigiusz
Published: July 2021

(1) Background: Regular participation in physical activity (PA) prevents many medical complications and improves the physical fitness of people with spinal cord injury, and in turn improves the functional independence, psychosocial status and quality of life. The goal of Active Rehabilitation Camps (ARCs) is to use various forms of PA in order for the participants to obtain the greatest efficiency and independence in everyday life. (2) Purpose: To evaluate the improvement in physical performance of people with chronic spinal cord disabilities moving in wheelchairs taking part in the Active Rehabilitation Camp depending on (a) sex, (b) type of disability, (c) the level of injury and (d) the type of wheelchair. (3) Methods: The study included 42 wheelchair users: 28 men and 14 women aged 18–65 years (34.7 ± 14.9 years) taking part in the Active Rehabilitation Camp. Finally, the study involved 27 paraplegics, 9 tetraplegics and 6 individuals with myelomeningocele. The participants took part in four fitness tests: (1) sprint test (SP)—individual time to cover a distance of 15 m in the wheelchair; (2) slalom test (SL)—time to ride between four cones front and back; (3) basketball ball throw at a distance (BT), (4) zig-zag test (ZZ)—riding continuously for 6 min on the designated track. The tests were performed at the beginning and at the end of the ACR. Active wheelchairs were used by 32 participants, and 10 participants used the classic wheelchairs. (4) Results: Paraplegics achieved the best average results in all the tests and the best improvement in physical performance in comparison to individuals with myelomeningocele and tetraplegics. People in active wheelchairs achieved a statistically significant improvement in the results of SL and ZZ (p < 0.001). People with injury above Th-9 level of the spinal cord achieved a statistically significant improvement in the results of SP (p < 0.01), SL and ZZ (p < 0.01). People with injury below Th6 achieved a statistically significant improvement (p < 0.05) in SP, SL and ZZ. (5) Conclusions: Regular PA during the Active Rehabilitation Camp improves the physical performance of disabled people in wheelchairs, but the scale of improvement of physical performance fitness depends on the type of wheelchair used and the level and the type of injury.
Physical Activity, Fitness, and Cognitive Performance of Estonian First-Grade Schoolchildren According Their MVPA Level in Kindergarten: A Longitudinal Study
Authors: Reisberg Kirkke , Riso Eva-Maria , Jürimäe Jaak
Published: July 2021

Little is known about the longitudinal trajectories and associations regarding physical activity (PA), physical fitness (PF), and cognitive skills in childhood. Accelerometer-based PA, sedentary behavior (SB), PF, and cognitive skills were measured in Estonian children (n = 147) in kindergarten (6.6 years) and again at school (7.6 years). Children were subgrouped into lower and upper quartiles by their moderate-to-vigorous PA (MVPA) at 6.6 years. Children in the upper quartile had lower SB, higher PA, and greater muscular strength. Attending school, MVPA in the lower quartile improved. In both subgroups, most strength values and cognitive skills improved, while balance deteriorated in first grade. In the upper quartile, a greater MPA at 6.6 years predicted lower perceptual skills at 7.6 years. A greater SB at 6.6 years predicted higher verbal skills, light and moderate PA and MVPA, and lower verbal skills at 7.6 years after taking into account confounding factors such as the child’s sex, age, awake wear time (AWT), maternal education, and/or child’s sports participation. A vigorous PA at 6.6 years predicted perceptual (in upper quartile) or verbal (in lower quartile) skills at 7.6 years after controlling for similar confounders. No correlation for PF at 6.6 years and cognitive skills at 7.6 years existed; after adjusting for the above-mentioned confounders relative to upper/lower-limb strength, the 4 × 10 m shuttle run results predicted higher perceptual or verbal skills; static balance and cardiorespiratory fitness predicted lower verbal skills. Cardiorespiratory fitness predicted higher perceptual skills after controlling for sex, age, and AWT. Overall, PA and strength were constantly better and SB lower in the upper quartile, yet the lower quartile demonstrated improved MVPA in first grade, and both subgroups increased most components of their strength and cognitive skills in first grade. Higher levels of VPA at kindergarten predicted either better perceptual or verbal skills in first grade after controlling for confounders; the opposite associations were found for other PA levels and cognitive skills in the higher quartile. PF components at kindergarten predicted either superior or inferior cognitive skills in first grade after adjusting for confounders.
Optimizing Green-Gray Infrastructure for Non-Point Source Pollution Control under Future Uncertainties
Authors: Dong Xinyu , Yuan Peng , Song Yonghui , Yi Wenxuan
Published: July 2021

Non-Point Source Pollution (NPS) caused by polluted and untreated stormwater runoff discharging into water bodies has become a serious threat to the ecological environment. Green infrastructure and gray infrastructure are considered to be the main stormwater management measures, and the issue of their cost-effectiveness is a widespread concern for decision makers. Multi-objective optimization is one of the most reliable and commonly used approaches in solving cost-effectiveness issues. However, many studies optimized green and gray infrastructure under an invariant condition, and the additional benefits of green infrastructure were neglected. In this study, a simulation-optimization framework was developed by integrated Stormwater Management Model (SWMM) and Non-dominated Sorting Genetic Algorithm (NSGA-II) to optimize green and gray infrastructure for NPS control under future scenarios, and a realistic area of Sponge City in Nanchang, China, was used as a typical case. Different levels of additional benefits of green infrastructure were estimated in the optimizing process. The results demonstrated that green-gray infrastructure can produce a co-benefit if the green infrastructure have appropriate Value of Additional Benefits (VAB), otherwise, gray infrastructure will be a more cost-effectiveness measure. Moreover, gray infrastructure is more sensitive than green infrastructure and green-gray infrastructure under future scenarios. The findings of the study could help decision makers to develop suitable planning for NPS control based on investment cost and water quality objectives.
Healthcare Professionals’ Perceptions of Function-Focused Care Education for Nursing Home Practitioners
Authors: Lee Su-Jung , Park Min-Sun , Chang Sung-Ok
Published: July 2021

A nursing home (NH) care environment necessitates a shared cognition-based education model that maintains effective function-focused care (FFC). This study’s aim was to explore healthcare professionals’ perceptions of function-focused care education for the development of an education model using a shared mental model (SMM) in NHs. Semi-structured interviews with 30 interdisciplinary practitioners from four different professions (nurses, physical therapists, occupational therapists, and social workers) and focus group interviews with 12 experts were conducted. Data were analyzed using content analysis, and the education model development was guided by the shared mental models for data interpretation and formation. Our FFC interdisciplinary educational model incorporates four key learning components: learning contents, educational activities, educational goals/outcome, and environment, and four types of SMMs: team, task, team interaction, and equipment. As for educational contents, a team’s competencies with FFC were found to be team knowledge (physical and psychosocial functional care), team skills to perform FFC successfully (motivation, coaching and supporting, managing discomfort), and team attitude (possessing philosophy perceptions regarding FFC). As for learning outcomes, the shared cognition-based education model suggests not only the evaluation of practitioners, but also the assessment of residents’ aspects.
An Investigation into Smartphone Addiction with Personality and Sleep Quality among University Students
Authors: Lane Hsien-Yuan , Chang Chin-Jui , Huang Chieh-Liang , Chang Yun-Hsuan
Published: July 2021

Over the past two decades, smartphones have become common, and the accompanying devices have also become much more popular and easily accessible worldwide. With the development of smartphones, accompanied by internet facilities, excessive smartphone use or smartphone addiction may cause sleep disturbance and daily dysfunction. This study proposed examining the association between personality traits and smartphone addiction and its effects on sleep disturbance. Four hundred and twenty-two university participants (80 male and 342 female participants) with a mean age of 20.22 years old were recruited in this study. All participants were asked to complete the following questionnaires: Smartphone Addiction Inventory (SPAI), Tri-dimensional personality questionnaire (TPQ), and Chinese Pittsburgh Sleep Questionnaire Index (CPSQI). The results showed that people with a high tendency toward novelty seeking (NS) as a personality trait, compared to those with lower tendency toward NS, are more likely to become addicted to smartphone use. Moreover, those with a stronger trait of being NS and specific impulsivity factor were found to have higher total scores in the SPAI (p < 0.05). In addition, linear regression analysis showed that the individuals with higher scores for withdrawal symptoms on the SPAI and anticipatory worry factor on the TPQ tended to have higher CPSQI total scores (p < 0.05). This information may be useful for prevention in individuals with personality traits making them vulnerable to smartphone addiction and for designing intervention programs to reduce intensive smartphone use and programs to increase capability in managing smartphone use.
Variability of Body Build and Physiological Spinal Curvatures of Young People in an Accelerated Longitudinal Study
Authors: Tuz Jacek , Maszczyk Adam , Zwierzchowska Anna
Published: July 2021

The human body is characterized by the variability of the characteristics of body build, which is expressed in the instability of spinal curvatures, which change during ontogeny. This phenomenon leads to a phylogenetic evolution of the human body build and posture. The aim of the study was to assess the dynamics of the variability of traits and indices of body build and posture and their mutual relations. It was assumed that over the 10-year observation period, a significant variability and relationships will be observed between the characteristics of body build and posture in young adults. Between 2006 and 2016, 2154 women and men aged 19.6 ± 0.8 were examined (first-year students at the University of Economics in Katowice, Poland). Measurements of basic anthropometric measurements and angular values of lumbar lordosis and thoracic kyphosis were performed. The collected data were analysed statistically, and the intergroup and intragroup differences were evaluated (ANOVA with repeated measures). The dynamics of variability (by building time series) and absolute and single-base increments were determined. The body build of young men and women in the period of ontogenetic stabilization in the study group has changed statistically significantly over the decade. A prediction of the increases in body weight and hip circumference was recorded over a period of 10 years, especially in men. The characteristics of body posture have also undergone a significant progressive change. In women, thoracic kyphosis increased by 96.15%, whereas in men, lumbar lordosis decreased by 52.65%. Significant sexual differentiation was shown for such characteristics as body height, waist circumference and the angle of lumbar lordosis. The relationships between the characteristics of body build and posture were verified. There was a moderate relationship between the hip circumference and lumbar lordosis in women and a weak relationship between body height and the angle of thoracic kyphosis in men.
Which National Factors Are Most Influential in the Spread of COVID-19?
Authors: Kim Hakyong , Apio Catherine , Ko Yeonghyeon , Han Kyulhee , Goo Taewan , Heo Gyujin , Kim Taehyun , Chung Hye Won , Lee Doeun , Lim Jisun , Park Taesung
Published: July 2021

The outbreak of the novel COVID-19, declared a global pandemic by WHO, is the most serious public health threat seen in terms of respiratory viruses since the 1918 H1N1 influenza pandemic. It is surprising that the total number of COVID-19 confirmed cases and the number of deaths has varied greatly across countries. Such great variations are caused by age population, health conditions, travel, economy, and environmental factors. Here, we investigated which national factors (life expectancy, aging index, human development index, percentage of malnourished people in the population, extreme poverty, economic ability, health policy, population, age distributions, etc.) influenced the spread of COVID-19 through systematic statistical analysis. First, we employed segmented growth curve models (GCMs) to model the cumulative confirmed cases for 134 countries from 1 January to 31 August 2020 (logistic and Gompertz). Thus, each country’s COVID-19 spread pattern was summarized into three growth-curve model parameters. Secondly, we investigated the relationship of selected 31 national factors (from KOSIS and Our World in Data) to these GCM parameters. Our analysis showed that with time, the parameters were influenced by different factors; for example, the parameter related to the maximum number of predicted cumulative confirmed cases was greatly influenced by the total population size, as expected. The other parameter related to the rate of spread of COVID-19 was influenced by aging index, cardiovascular death rate, extreme poverty, median age, percentage of population aged 65 or 70 and older, and so forth. We hope that with their consideration of a country’s resources and population dynamics that our results will help in making informed decisions with the most impact against similar infectious diseases.
Organizational-Level Moderators Impacting Tobacco-Related Knowledge Change after Tobacco Education Training in Substance Use Treatment Centers
Authors: Le Kathy , Chen Tzuan A. , Martinez Leal Isabel , Correa-Fernández Virmarie , Obasi Ezemenari M. , Kyburz Bryce , Williams Teresa , Casey Kathleen , Brown Haleem A. , O’Connor Daniel P. , Reitzel Lorraine R.
Published: July 2021

Tobacco use is disproportionately elevated among patients with substance use disorders relative to the general U.S. population. Tobacco interventions are lacking within substance use treatment centers (SUTCs) due to lack of knowledge and training. This study examined knowledge gain and the organizational factors that might moderate knowledge gains following tobacco education training provided to employees (N = 580) within 15 SUTCs that were participating in a tobacco-free workplace program. The number of total annual patient visits, unique annual patient visits, number of full-time employees, and organizational readiness for implementing change (ORIC) as assessed prior to implementation were examined as potential moderators. Results demonstrated significant knowledge gain (p < 0.001) after training overall; individually, 13 SUTCs had significant knowledge gain (p’s < 0.014). SUTCs with fewer total annual patient visits and fewer full-time employees showed greater knowledge gains. The ORIC total score and all but one of its subscales (Resource Availability) moderated knowledge gain. SUTCs with greater initial Change Efficacy (p = 0.029), Valence (p = 0.027), and Commitment (p < 0.001) had greater knowledge gain than SUTCs with lower scores on these constructs; SUTCs with greater Task Knowledge (p < 0.001) regarding requirements for change exhibited less knowledge gain. Understanding the organizational-level factors impacting training effectiveness can inform efforts in organizational change and tobacco control program implementation.
Heart Metabolism in Sepsis-Induced Cardiomyopathy—Unusual Metabolic Dysfunction of the Heart
Authors: Wasyluk Weronika , Nowicka-Stążka Patrycja , Zwolak Agnieszka
Published: July 2021

Due to the need for continuous work, the heart uses up to 8% of the total energy expenditure. Due to the relatively low adenosine triphosphate (ATP) storage capacity, the heart’s work is dependent on its production. This is possible due to the metabolic flexibility of the heart, which allows it to use numerous substrates as a source of energy. Under normal conditions, a healthy heart obtains approximately 95% of its ATP by oxidative phosphorylation in the mitochondria. The primary source of energy is fatty acid oxidation, the rest of the energy comes from the oxidation of pyruvate. A failed heart is characterised by a disturbance in these proportions, with the contribution of individual components as a source of energy depending on the aetiology and stage of heart failure. A unique form of cardiac dysfunction is sepsis-induced cardiomyopathy, characterised by a significant reduction in energy production and impairment of cardiac oxidation of both fatty acids and glucose. Metabolic disorders appear to contribute to the pathogenesis of cardiac dysfunction and therefore are a promising target for future therapies. However, as many aspects of the metabolism of the failing heart remain unexplained, this issue requires further research.
Effect of Water Amount Intake before Scuba Diving on the Risk of Decompression Sickness
Authors: Han Kil-Hyung , Hyun Gwang-Suk , Jee Yong-Seok , Park Jung-Min
Published: July 2021

Background and objective: The aim of this study was to investigate the influence of pre-hydration levels on circulating bubble formation for scuba divers and to evaluate the appropriate volume of water intake for reducing the risk of decompression sickness (DCS). Materials and Methods: Twenty scuba divers were classified into four groups according to the volume of water taken in before scuba diving as follows: no-water-intake group (NWIG), 30%-water-intake group (30WIG), 50%-water intake group (50WIG), and 100%-water-intake group (100WIG). We measured the circulating bubbles using movement status by Doppler on the right and left subclavian veins and precordial regions at pre-dive, post-dive, and 30 min after diving to a depth of 30 m for a duration of 25 min at the bottom. Results: Participants belonging to the 30WIG showed the lowest frequency, percentage, and amplitude of bubbles and consequently the lowest bubble grade in the left and right subclavian veins and precordial region at post-time and 30 min after diving. Conclusions: It can be inferred that pre-hydration with 30% of the recommended daily water intake before scuba diving effectively suppressed the formation of bubbles after diving and decreased the risk of DCS.
Microbial Synthesis and Evaluation of Fungistatic Activity of 3-Butyl-3-hydroxyphthalide, the Mammalian Metabolite of 3-n-Butylidenephthalide
Authors: Gach Joanna , Olejniczak Teresa , Krężel Piotr , Boratyński Filip
Published: July 2021

Phthalides are bioactive compounds that naturally occur in the family Apiaceae. Considering their potentially versatile applications, it is desirable to determine their physical properties, activity and metabolic pathways. This study aimed to examine the utility of whole-cell biocatalysts for obtaining 3-butyl-3-hydroxyphthalide, which is the metabolite formulated during mammalian metabolism of 3-n-butylidenephthalide. We performed transformations using 10 strains of fungi, five of which efficiently produced 3-butyl-3-hydroxyphthalide. The product yield, determined by high-performance liquid chromatography, reached 97.6% when Aspergillus candidus AM 386 was used as the biocatalyst. Increasing the scale of the process resulted in isolation yields of 29–45% after purification via reversed-phase thin layer chromatography, depending on the strain of the microorganism used. We proposed different mechanisms for product formation; however, hydration of 3-n-butylidenephthalide seems to be the most probable. Additionally, all phthalides were tested against clinical strains of Candida albicans using the microdilution method. Two phthalides showed a minimum inhibitory concentration, required to inhibit the growth of 50% of organisms, below 50 µg/mL. The 3-n-butylidenephthalide metabolite was generally inactive, and this feature in combination with its low lipophilicity suggests its involvement in the detoxification pathway. The log P value of tested compounds was in the range of 2.09–3.38.
Architecture of the Pancreatic Islets and Endocrine Cell Arrangement in the Embryonic Pancreas of the Grass Snake (Natrix natrix L.). Immunocytochemical Studies and 3D Reconstructions
Authors: Kowalska Magdalena , Rupik Weronika
Published: July 2021

During the early developmental stages of grass snakes, within the differentiating pancreas, cords of endocrine cells are formed. They differentiate into agglomerates of large islets flanked throughout subsequent developmental stages by small groups of endocrine cells forming islets. The islets are located within the cephalic part of the dorsal pancreas. At the end of the embryonic period, the pancreatic islet agglomerates branch off, and as a result of their remodeling, surround the splenic “bulb”. The stage of pancreatic endocrine ring formation is the first step in formation of intrasplenic islets characteristics for the adult specimens of the grass snake. The arrangement of endocrine cells within islets changes during pancreas differentiation. Initially, the core of islets formed from B and D cells is surrounded by a cluster of A cells. Subsequently, A, B, and D endocrine cells are mixed throughout the islets. Before grass snake hatching, A and B endocrine cells are intermingled within the islets, but D cells are arranged centrally. Moreover, the pancreatic polypeptide (PP) cells are not found within the embryonic pancreas of the grass snake. Variation in the proportions of different cell types, depending on the part of the pancreas, may affect the islet function—a higher proportion of glucagon cells is beneficial for insulin secretion.
Sodium Intake and Related Diseases
Authors: Lucarini Massimo , Durazzo Alessandra , Sette Stefania , Lombardi-Boccia Ginevra , Santini Antonello , Strazzullo Pasquale
Published: July 2021

Moderation in the use of salt (sodium chloride) in food and food preparations prevents the tendency of blood pressure to increase with age, and this is documented by many studies in current literature [...]
Leaky Gut: Effect of Dietary Fiber and Fats on Microbiome and Intestinal Barrier
Authors: Usuda Haruki , Okamoto Takayuki , Wada Koichiro
Published: July 2021

Intestinal tract is the boundary that prevents harmful molecules from invading into the mucosal tissue, followed by systemic circulation. Intestinal permeability is an index for intestinal barrier integrity. Intestinal permeability has been shown to increase in various diseases—not only intestinal inflammatory diseases, but also systemic diseases, including diabetes, chronic kidney dysfunction, cancer, and cardiovascular diseases. Chronic increase of intestinal permeability is termed ‘leaky gut’ which is observed in the patients and animal models of these diseases. This state often correlates with the disease state. In addition, recent studies have revealed that gut microbiota affects intestinal and systemic heath conditions via their metabolite, especially short-chain fatty acids and lipopolysaccharides, which can trigger leaky gut. The etiology of leaky gut is still unknown; however, recent studies have uncovered exogenous factors that can modulate intestinal permeability. Nutrients are closely related to intestinal health and permeability that are actively investigated as a hot topic of scientific research. Here, we will review the effect of nutrients on intestinal permeability and microbiome for a better understanding of leaky gut and a possible mechanism of increase in intestinal permeability.
LC-ESI-QTOF-MS/MS Analysis, Cytotoxic, Antiviral, Antioxidant, and Enzyme Inhibitory Properties of Four Extracts of Geranium pyrenaicum Burm. f.: A Good Gift from the Natural Treasure
Authors: Świątek Łukasz , Sieniawska Elwira , Sinan Kouadio Ibrahime , Maciejewska-Turska Magdalena , Boguszewska Anastazja , Polz-Dacewicz Małgorzata , Senkardes Ismail , Guler Gokalp Ozmen , Bibi Sadeer Nabeelah , Mahomoodally Mohamad Fawzi , Zengin Gokhan
Published: July 2021

This study focused on the biological evaluation and chemical characterization of Geranium pyrenaicum Burm. f. Different solvent extracts (hexane, ethyl acetate, methanol, and water extracts) were prepared. The phytochemical profile, antioxidant, and enzyme inhibitory activity were investigated. Cytotoxicity was assessed using VERO, FaDu, HeLa and RKO cells. The antiviral activity was carried out against HSV-1 (Herpes simplex virus 1) propagated in VERO cell line. The aqueous extract, possessing high phenolic content (170.50 mg gallic acid equivalent/g extract), showed the highest reducing capacity (613.27 and 364.10 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively), radical scavenging potential (469.82 mg Trolox equivalent/g extract, against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), metal chelating ability (52.39 mg ethylenediaminetetraacetic acid equivalent/g extract) and total antioxidant capacity (3.15 mmol Trolox equivalent/g extract). Liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS) alloved to tentatively identify a total of 56 compounds in the extracts, including ellagitannins, gallic acid and galloyl derivatives amongst others. The ethyl acetate extracts substantially depressed cholinesterase enzymes (4.49 and 12.26 mg galantamine equivalent/g extract against AChE and BChE, respectively) and α-amylase enzyme (1.04 mmol acarbose equivalent/g extract). On the other hand, the methanolic extract inhibited tyrosinase (121.42 mg kojic acid equivalent/g extract) and α-glucosidase (2.39 mmol acarbose equivalent/g extract) activities. The highest selectivity towards all cancer cell lines (SI 4.5–10.8) was observed with aqueous extract with the FaDu cells being the most sensitive (CC50 40.22 µg/mL). It can be concluded that the presence of certain bioactive antiviral molecules may be related to the high anti HSV-1 activity of the methanolic extract. This work has generated vital scientific data on this medicinal plant, which is a prospective candidate for the creation of innovative phyto-pharmaceuticals.
Ex Vivo and In Vivo Analysis of a Novel Porcine Aortic Patch for Vascular Reconstruction
Authors: Stöwe Ignacio , Pissarek Jens , Moosmann Pia , Pröhl Annica , Pantermehl Sven , Bielenstein James , Radenkovic Milena , Jung Ole , Najman Stevo , Alkildani Said , Barbeck Mike
Published: July 2021

(1) Background: The aim of the present study was the biocompatibility analysis of a novel xenogeneic vascular graft material (PAP) based on native collagen won from porcine aorta using the subcutaneous implantation model up to 120 days post implantationem. As a control, an already commercially available collagen-based vessel graft (XenoSure®) based on bovine pericardium was used. Another focus was to analyze the (ultra-) structure and the purification effort. (2) Methods: Established methodologies such as the histological material analysis and the conduct of the subcutaneous implantation model in Wistar rats were applied. Moreover, established methods combining histological, immunohistochemical, and histomorphometrical procedures were applied to analyze the tissue reactions to the vessel graft materials, including the induction of pro- and anti-inflammatory macrophages to test the immune response. (3) Results: The results showed that the PAP implants induced a special cellular infiltration and host tissue integration based on its three different parts based on the different layers of the donor tissue. Thereby, these material parts induced a vascularization pattern that branches to all parts of the graft and altogether a balanced immune tissue reaction in contrast to the control material. (4) Conclusions: PAP implants seemed to be advantageous in many aspects: (i) cellular infiltration and host tissue integration, (ii) vascularization pattern that branches to all parts of the graft, and (iii) balanced immune tissue reaction that can result in less scar tissue and enhanced integrative healing patterns. Moreover, the unique trans-implant vascularization can provide unprecedented anti-infection properties that can avoid material-related bacterial infections.
Expression of DDX11 and DNM1L at the 12p11 Locus Modulates Systemic Lupus Erythematosus Susceptibility
Authors: Saeed Mohammad , Ibáñez-Costa Alejandro , Patiño-Trives Alejandra María , Muñoz-Barrera Laura , Collantes Estévez Eduardo , Aguirre María Ángeles , López-Pedrera Chary
Published: July 2021

Objectives: This study employed genetic and functional analyses using OASIS meta-analysis of multiple existing GWAS and gene-expression datasets to identify novel SLE genes. Methods: Four hundred and ten genes were mapped using SNIPPER to 30 SLE GWAS loci and investigated for expression in three SLE GEO-datasets and the Cordoba GSE50395-dataset. Blood eQTL for significant SNPs in SLE loci and STRING for functional pathways of differentially expressed genes were used. Confirmatory qPCR on SLE monocytes was performed. The entire 12p11 locus was investigated for genetic association using two additional GWAS. Expression of 150 genes at this locus was assessed. Based on this significance, qPCRs for DNM1L and KRAS were performed. Results: Fifty genes were differentially expressed in at least two SLE GEO-datasets, with all probes directionally aligned. DDX11, an RNA helicase involved in genome stability, was downregulated in both GEO and Cordoba datasets. The most significant SNP, rs3741869 in OASIS locus 12p11.21, containing DDX11, was a cis-eQTL regulating DDX11 expression. DDX11 was found repressed. The entire 12p11 locus showed three association peaks. Gene expression in GEO datasets identified DNM1L and KRAS, besides DDX11. Confirmatory qPCR validated DNM1L as an SLE susceptibility gene. DDX11, DNM1L and KRAS interact with each other and multiple known SLE genes including STAT1/STAT4 and major components of IFN-dependent gene expression, and are responsible for signal transduction of cytokines, hormones, and growth-factors, deregulation of which is involved in SLE-development. Conclusion: A genomic convergence approach with OASIS analysis of multiple GWAS and expression datasets identified DDX11 and DNM1L as novel SLE-genes, the expression of which is altered in monocytes from SLE patients. This study lays the foundation for understanding the pathogenic involvement of DDX11 and DNM1L in SLE by identifying them using a systems-biology approach, while the 12p11 locus harboring these genes was previously missed by four independent GWAS.
Enhancing the Effect of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Signaling and Arginine Deprivation in Melanoma
Authors: Wu Chunjing , You Min , Nguyen Dao , Wangpaichitr Medhi , Li Ying-Ying , Feun Lynn G. , Kuo Macus T. , Savaraj Niramol
Published: July 2021

Melanoma as a very aggressive type of cancer is still in urgent need of improved treatment. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and arginine deiminase (ADI-PEG20) are two of many suggested drugs for treating melanoma. Both have shown anti-tumor activities without harming normal cells. However, resistance to both drugs has also been noted. Studies on the mechanism of action of and resistance to these drugs provide multiple targets that can be utilized to increase the efficacy and overcome the resistance. As a result, combination strategies have been proposed for these drug candidates with various other agents, and achieved enhanced or synergistic anti-tumor effect. The combination of TRAIL and ADI-PEG20 as one example can greatly enhance the cytotoxicity to melanoma cells including those resistant to the single component of this combination. It is found that combination treatment generally can alter the expression of the components of cell signaling in melanoma cells to favor cell death. In this paper, the signaling of TRAIL and ADI-PEG20-induced arginine deprivation including the main mechanism of resistance to these drugs and exemplary combination strategies is discussed. Finally, factors hampering the clinical application of both drugs, current and future development to overcome these hurdles are briefly discussed.