bluuebunny/crossref_metadata_2025 huggingface dataset:
Wake Effect of a Horizontal Axis Wind Turbine on the Performance of a Downstream Turbine
Authors: Tang Haojun , Lam Kit-Ming , Shum Kei-Man , Li Yongle
Published: June 2019

This paper presents wind tunnel tests on the wake characteristics of a three-blade horizontal axis wind turbine and the wake effect on the performance of a downstream turbine. For a single turbine model, the performance was determined and this was followed by measurement of the wind characteristics including velocities, turbulence intensities, and correlation in the wake flow field. Subsequently, taking two horizontal axis wind turbines in a tandem arrangement into account, their performance was tested and the aerodynamic mechanism was discussed. The results showed that the upstream turbine with blades set at a small pitch angle provided smaller disturbance to the flow, but as the blade turned faster, larger changes in the velocity and the turbulence intensity occurred in its wake due to the more frequent disturbance of the wind turbine. The correlation of wake velocities in the turbine swept area also obviously decreased from the free-stream situation. For the downstream turbine, the output power loss largely depended on the wake characteristics of the upstream turbine, which was closely related to lower wind velocities or higher turbulence intensities. The decrease in correlation of the streamwise velocity within the blade swept area is accompanied by the increased correlation of the tangential velocity, which may be beneficial to the downstream turbine’s performance.
Model Analysis of Electrically Driven Vehicles by Means of Unknown Input Observers
Authors: Kulikov Ilya
Published: June 2019

The article describes a method to analyze the powertrain operation of electrically driven vehicles in cases of insufficient information (i.e., unknown control algorithms, no torque measurements during vehicle tests). The method implies mathematical modeling with involvement of so-called unknown input observers. A variant of such an observer is proposed. Using that observer, studies of a hybrid vehicle and a pure electric vehicle are performed. The models with torque observers simulated tests of said vehicles conducted on a chassis dynamometer and on roads. For the hybrid vehicle, operating regimes of main powertrain components were identified. For the electric vehicle, the identification revealed a coordinated operation of regenerative braking and mechanical braking. Adequacy of the modeling, including identification of the unmeasured torques, was verified through a comparison with experimental data.
Virtual Power Plant Management in Smart Grids with XMPP Based IEC 61850 Communication
Authors: Nadeem Furquan , Aftab Mohd Asim , Hussain S.M. Suhail , Ali Ikbal , Tiwari Prashant Kumar , Goswami Arup Kumar , Ustun Taha Selim
Published: June 2019

Communication plays a key role in the effective management of virtual power plants (VPPs). For effective and stable operation of VPPs, a reliable, secure, and standardized communication infrastructure is required. In the literature, efforts were made to develop this based on industry standards, such as the IEC 60870-5-104, OpenADR 2.0b and IEC 61850. Due to its global acceptance and strong object-oriented information models, IEC 61850 standard-based communication is preferred for smart grid operations, including VPPs. However, communication models based on IEC 61850 present cybersecurity and scalability challenges. To address this issue, this paper presents an eXtensible Message Presence Protocol (XMPP)-based IEC 61850 communication for VPPs. Firstly, a full mapping of IEC 61850 messages for VPP energy management is carried out. Secondly, XMPP-based single- and multiple-domain communications are demonstrated. Finally, a federation concept has been added to facilitate communication in multi-domain communication networks. These models show that a standard communication model can be implemented with IEC 61850 and XMPP, not only for VPPs but other wide-area communication implementations in smart grids. This not only facilitates plug-and-play (PnP) with easy component additions but secures smart grid communication against cyber-attacks.
Impacts of an Increased Substitution of Fossil Energy Carriers with Electricity-Based Technologies on the Swiss Electricity System
Authors: Rüdisüli Martin , Teske Sinan L. , Elber Urs
Published: June 2019

Electrifying the energy system with heat pumps and battery electric vehicles (BEV) is a strategy of Switzerland and many other countries to reduce CO2 emissions. A large electrification, however, poses several new challenges for the electricity system, particularly in combination with a simultaneous substitution of nuclear power plants (NPP) by volatile renewables such as photovoltaics (PV). In this study, these challenges in terms of additional electricity demands, deficits and surpluses as well as effective CO2 mitigation are assessed in a dynamic and data-driven approach. To this end, electricity demand and production profiles are synthesized based on measured data and specifications and assumptions of the key technologies at a high temporal resolution. The additional electricity demand of heat pumps is estimated from hourly measured heat demand profiles of a Swiss district heating provider, while for BEV different recharging patterns are combined. For electricity production, NPP are deducted from the current electricity production profile, while PV is added at an hourly resolution. In order to estimate CO2 emissions, life-cycle analysis (LCA) CO2 intensities of the different technologies are used. It is shown that with a BEV and heat pump penetration of 20% and 75%, respectively, there is an almost 25% (13.7 TWh/year) increase of the electricity demand and—just as challenging—an additional maximum power requirement of 5.9 GWh/h (hourly-averaged power). Without additional storage options, large amounts of electricity must be imported in winter and at night, while in summer at noon there is a large surplus from PV. Due to their high CO2 intensities—at least for the next decades—electricity imports and PV may—depending on the reference scenario (with or without NPP) and assumptions on other key parameters—even offset the overall CO2 savings of a highly electrified Swiss energy system.
Determination of the Insulation Solution that Leads to Lower CO2 Emissions during the Construction Phase of a Building
Authors: Bastante-Ceca María José , Cerezo-Narváez Alberto , Piñero-Vilela José-María , Pastor-Fernández Andrés
Published: June 2019

The characteristics of the envelope of a building determine, together with other factors, its consumption of energy. Additionally, the climate zone and insulation material may vary the minimum insulation thickness of walls and roofs, making it different, according to cooling down or warming up the home. Spanish legislation establishes different maximum values for energy demand according to different climate area both for heating and for cooling. This paper presents the results of a study that determines the influence of many variables as the climate zone or the orientation, among others, in the optimization of thickness insulation in residential homes in Spain to reduce the CO2 emissions embodied. To do that, 12 representative cities in Spain corresponding to different climate zones, four orientations, two constructive solutions, and four different configurations of the same house have been combined, for three different hypotheses and four insulation materials, resulting in 4608 cases of study. The results show that, under equal conditions on energy demand, the optimal insulation requirements are determined by heating necessities more than by cooling ones. In addition, a higher insulation thickness need does not necessarily mean more CO2 emissions, since it can be compensated with a lower Global Warming Potential characterization factor that is associated to the insulation material. The findings of this study can serve to designers and architects to establish the better combination of the variables that are involved in order to minimize the CO2 emissions embodied during the construction phase of a building, making it more energy efficient.
Current Status of Las Tablas de Daimiel National Park Wetland and Actions Required for Conservation
Authors: Bravo-Martin Sandra , Mejías Miguel , García-Navarro Francisco J. , Jiménez-Ballesta Raimundo
Published: June 2019

Wetlands are complex ecosystems that play multiple roles. ‘Las Tablas de Daimiel National Park’ (TDNP) undoubtedly plays a role in several ecosystem services and provides a connection between nature, farmers, scientists, residents, and other stakeholders. The state of degradation and/or vulnerability of this ecosystem (with a series of socio-economic implications) have led the publication of numerous articles. The work reported here provides a description of the growing importance of this wetland within the rural landscapes of La Mancha and emphasizes its state of degradation, mainly since pedological point of view. In this way, particular attention is required to assure the conservation of the Tablas of Daimiel Wetland; thus, several measures are proposed to improve the conservation of this area as to control and prohibit any dumping of any type of waste in the park or in its vicinity.
Effect of Sugar Content on Quality Characteristics and Shelf-Life of Probiotic Dry-Fermented Sausages Produced by Free or Immobilized Lactobacillus casei ATCC 393
Authors: Sidira Marianthi , Mitropoulou Gregoria , Galanis Alex , Kanellaki Maria , Kourkoutas Yiannis
Published: June 2019

The aim of the present study was to investigate the effect of sugar content (0, 0.30, and 0.60%) on quality attributes and shelf-life of dry-fermented sausages stored for 66 days containing free or immobilized Lactobacillus casei ATCC 393 on wheat. For comparison, dry-fermented sausages with no starter culture were also produced. Physicochemical characteristics ranged within the levels usually observed in fermented sausages, while a drastic decrease was recorded in numbers of enterobacteria, staphylococci, and pseudomonads during ripening in all cases. Noticeably, sugar addition and the probiotic culture resulted in significant increase of shelf-life, whereas levels of L. casei ATCC 393 after 66 days of ripening persisted above 6 log cfu/g. Sugar addition had a positive effect on sensory attributes; although all products were of high quality, the immobilized cells provided a distinctive characteristic aroma and a fine taste.
Genome-Wide Identification and Analysis of Class III Peroxidases in Allotetraploid Cotton (Gossypium hirsutum L.) and their Responses to PK Deficiency
Authors: Duan Pengfei , Wang Guo , Chao Maoni , Zhang Zhiyong , Zhang Baohong
Published: June 2019

Class III peroxidases (PODs), commonly known as secretable class III plant peroxidases, are plant-specific enzymes that play critical roles in not only plant growth and development but also the responses to biotic and abiotic stress. In this study, we identified 198 nonredundant POD genes, designated GhPODs, with 180 PODs being predicted to secrete into apoplast. These POD genes were divided into 10 sub-groups based on their phylogenetic relationships. We performed systematic bioinformatic analysis of the POD genes, including analysis of gene structures, phylogenetic relationships, and gene expression profiles. The GhPODs are unevenly distributed on both upland cotton sub-genome A and D chromosomes. Additionally, these genes have undergone 15 segmental and 12 tandem duplication events, indicating that both segmental and tandem duplication contributed to the expansion of the POD gene family in upland cotton. Ka/Ks analysis suggested that most duplicated GhPODs experienced negative selection, with limited functional divergence during the duplication events. High-throughput RNA-seq data indicated that most highly expressed genes might play significant roles in root, stem, leaf, and fiber development. Under K or P deficiency conditions, PODs showed different expression patterns in cotton root and leaf. This study provides useful information for further functional analysis of the POD gene family in upland cotton.
Geomorphological Evolution of the Sena Gallica Site in the Morpho-Evolutive Quaternary Context of the Northern-Marche Coastal Sector (Italy)
Authors: De Donatis Mauro , Nesci Olivia , Savelli Daniele , Pappafico Giulio F. , Susini Sara
Published: June 2019

The Sena Gallica Roman town was settled on the Adriatic coast in the 5th to 4th century BC. The choice of the site was largely influenced by the geomorphological and physiographic conditions near the Misa river mouth. The interactions among climate variation, river dynamics, and marine oscillation determined the anthropic development. At the same time, the new settlement strongly influenced the evolution of this sector in both medieval and in recent times. This work aims to highlight the geological setting and geomorphological evolution of the Senigallia area within the Northern Marche region, taking into account the main scientific literature and new studies to propose a new interpretation of the Holocene history.
Relationship Between L4/5 Lumbar Multifidus Cross-Sectional Area Ratio and Fall Risk in Older Adults with Lumbar Spinal Stenosis: A Retrospective Study
Authors: Ito Tadashi , Sakai Yoshihito , Yamazaki Kazunori , Oikawa Makoto , Morita Yoshifumi
Published: June 2019

Various factors, including spinal deformities and trunk muscle atrophy, greatly affect the fall risk among older adults with lumbar spinal stenosis (LSS). However, the etiology of falls in older adults with degenerative LSS and trunk muscle atrophy is poorly understood. We investigated the association between trunk muscle atrophy and falls in older LSS patients. This retrospective study included 99 hospitalized older adults with LSS. Participants completed self-reported fall score questionnaires and were divided into the fall risk (n = 30) and non-fall risk (n = 69) groups. The patients’ low back pain visual analog scale score, Geriatric Depression Scale score, sagittal vertical axis, L4/5 lumbar multifidus cross-sectional area ratio (LMCSAR), and center of pressure (COP) values during quiet standing were evaluated. The fall risk group had a lower L4/5 LMCSAR (p = 0.002) and increased COP excursion (p = 0.034) than the non-fall risk group. No significant differences were observed in the other measured variables between the two groups. The L4/5 LMCSAR (p < 0.001) and COP (p = 0.024) were related to fall risk and may be useful in fall risk assessment in such populations. Strategies aimed at enhancing controlled lumbar segmental motion and improving trunk muscle stability or mass may decrease the fall risk in this cohort.
Consumption of Sugar-Sweetened Beverages Has a Dose-Dependent Effect on the Risk of Non-Alcoholic Fatty Liver Disease: An Updated Systematic Review and Dose-Response Meta-Analysis
Authors: Chen Hongwei , Wang Jue , Li Zheng , Lam Christopher Wai Kei , Xiao Ying , Wu Qibiao , Zhang Wei
Published: June 2019

Background: Non-alcoholic fatty liver disease (NAFLD) is a serious health problem, but the dose-response relationship between sugar-sweetened beverages (SSBs) and NAFLD remains uncertain. Methods: A systematic review and dose-response meta-analysis were conducted following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Review Manager 5.3 and Stata 14.0 were used to combine trials and analyze data. The dose-response meta-analysis was performed by non-linear trend regression. Results: Twelve studies recruiting a total of 35,705 participants were included. The results showed that the consumption of SSBs was associated with 1.39-fold increased odds of NAFLD (95% CI: 1.29–1.50, p < 0.00001). The risk of NAFLD rose with an increased consumption of SSBs, while the consumptions of low doses (<1 cup/week), middle doses (1–6 cups/week) and high doses (≥7 cups/week) of SSBs increased the relative risk of NAFLD by 14%, 26% and 53%, respectively (p = 0.01, p < 0.00001, p = 0.03, respectively). Conclusions: This study demonstrates that consumers of SSBs are at significantly increased risk of NAFLD, and the consumption of SSBs has a dose-dependent effect on the risk of NAFLD. The findings of this study strengthen the evidence base for healthy dietary patterns and are meaningful for the primary prevention of NAFLD.
Lighting Environment Optimization of Highway Tunnel Entrance Based on Simulation Research
Authors: Zhang Yongqiang , Zhuo Xi , Guo Wei , Wang Xiaoyu , Zhao Zhenglu
Published: June 2019

At the entrance of a tunnel, reflection of sunlight from the surrounding environment and a lack of adequate lighting usually cause some vision problems. The purpose of this study is to optimize the lighting environment at the entrance of highway tunnels. Firstly, based on a highway tunnel in Zhejiang Province, the natural illumination intensity in different seasons and climate conditions inside and outside the tunnel entrance was analyzed by means of DIALux simulation software. Then the variation in illumination conditions with distance at the entrance of the tunnel was analyzed. Finally, based on the results above, this study proposes four solutions as follows: setting up a shading shed, auxiliary lighting facilities, decelerating reflective markings, and an adaptive dimming system.
What Can Meal Observations Tell Us about Eating Behavior in Malnourished Children?
Authors: Mutoro Antonina N. , Garcia Ada L. , Wright Charlotte M.
Published: June 2019

Responsive feeding is an important aspect of child care, yet little is known about child eating and caregiver feeding behavior in Kenya. This study aimed to develop a mealtime observation methodology and assess child eating and caregiver feeding behavior in healthy and undernourished children in Nairobi. Healthy (n = 6) and undernourished (n = 13) children aged 6–24 months were observed during a meal, with standardized rating of child interest in food, mood, distraction and caregiver responsiveness. Eating and feeding behavior varied with the stage of the meal. Child interest in food decreased and child and caregiver distraction increased as the meal progressed. Healthy children were happy and interested in food during meals, but undernourished children often had low interest in food (7/13). The 7 undernourished children eating home food were distracted (3) and unhappy (5) but children eating ready-to-use therapeutic foods (6) were all happy and undistracted. Caregivers of healthy children offered encouragement more often during meals than caregivers of undernourished children (5/6 healthy, 3/13 undernourished). Meal observations were resource intensive and could give only a snapshot of the child feeding experience. More efficient research methods that can capture a general assessment of infant eating behavior are needed.
Efficiency of Health Care Risk Waste Management in Rural Healthcare Facilities of South Africa: An Assessment of Selected Facilities in Vhembe District, Limpopo Province
Authors: Olaniyi Foluke C. , Ogola Jason S. , Tshitangano Takalani G.
Published: June 2019

Waste generated form healthcare facilities is a potential source of health risks to the public, if it is not properly handled from the point of generation to disposal. This study was conducted to assess the efficiency of healthcare risk waste (HCRW) management in Vhembe District of Limpopo Province, South Africa. Fifteen healthcare facilities were selected in Vhembe District for this study. Data were obtained through in-depth interviews, semi-structured questionnaires, observation and pictures. Qualitative data were thematically analyzed, while the quantitative data were analyzed using the Statistical Package for the Social Sciences, version 25. In all the healthcare facilities; mismanagement of HCRW was noted at different points along the management chain. Poor segregation, overfilling of waste bins, inappropriate transportation and storage of waste in substandard storage rooms were observed in the facilities. All the waste from the district are transported to a private-owned treatment facility outside the district, where they are mainly incinerated. Enforcement of healthcare risk waste guidelines, provision of standardized equipment for temporary storage, empowerment of each healthcare facility to treat at least some of the waste, and employment of non-burn techniques for treatment of waste are recommended for more efficient management of healthcare risk waste in Vhembe District.
Determinants of Willingness of Patients with Type 2 Diabetes Mellitus to Receive the Seasonal Influenza Vaccine in Southeast China
Authors: Feng Wei , Cui Jun , Li Hui
Published: June 2019

Willingness of patients with Type 2 diabetes mellitus (T2DM) to receive the seasonal influenza vaccine is low in China. A cross-sectional study on a representative sample of T2DM patients was conducted in Ningbo, a city in southeast China, to assess T2DM patients’ willingness to be vaccinated against influenza and identify the influence factors of this willingness. Data regarding the participant’s history of influenza, the knowledge, willingness and uptake of the influenza vaccine, demographic characteristics, reasons for willingness or unwillingness to be vaccinated was collected. Only 19.55% of a total of 1749 participants reported a willingness to be vaccinated. Factors positively associated with willingness to be vaccinated were perceived susceptibility to influenza (OR = 1.9, 95% CI: 1.5–2.5), awareness of the vaccine (OR = 1.7, 95% CI: 1.3–2.3) and previous history of influenza vaccination (OR = 4.4, 95% CI: 3.0–6.4). Patients with T2DM who were farmers (OR = 0.6, 95% CI: 0.4–0.8) and those managed by contracted family doctors (OR = 0.8, 95% CI: 0.6–1.0) expressed less willingness to be vaccinated. Targeted interventions such as enhancing health education and strengthening medical staff training should be conducted to increase T2DM patients’ willingness to be vaccinated and enhance influenza vaccine uptake among this population.
A Population Dynamic Model to Assess the Diabetes Screening and Reporting Programs and Project the Burden of Undiagnosed Diabetes in Thailand
Authors: Mahikul Wiriya , J White Lisa , Poovorawan Kittiyod , Soonthornworasiri Ngamphol , Sukontamarn Pataporn , Chanthavilay Phetsavanh , Pan-ngum Wirichada , F Medley Graham
Published: June 2019

Diabetes mellitus (DM) is rising worldwide, exacerbated by aging populations. We estimated and predicted the diabetes burden and mortality due to undiagnosed diabetes together with screening program efficacy and reporting completeness in Thailand, in the context of demographic changes. An age and sex structured dynamic model including demographic and diagnostic processes was constructed. The model was validated using a Bayesian Markov Chain Monte Carlo (MCMC) approach. The prevalence of DM was predicted to increase from 6.5% (95% credible interval: 6.3–6.7%) in 2015 to 10.69% (10.4–11.0%) in 2035, with the largest increase (72%) among 60 years or older. Out of the total DM cases in 2015, the percentage of undiagnosed DM cases was 18.2% (17.4–18.9%), with males higher than females (p-value < 0.01). The highest group with undiagnosed DM was those aged less than 39 years old, 74.2% (73.7–74.7%). The mortality of undiagnosed DM was ten-fold greater than the mortality of those with diagnosed DM. The estimated coverage of diabetes positive screening programs was ten-fold greater for elderly compared to young. The positive screening rate among females was estimated to be significantly higher than those in males. Of the diagnoses, 87.4% (87.0–87.8%) were reported. Targeting screening programs and good reporting systems will be essential to reduce the burden of disease.
OsMKK3, a Stress-Responsive Protein Kinase, Positively Regulates Rice Resistance to Nilaparvata lugens via Phytohormone Dynamics
Authors: Zhou Shuxing , Chen Mengting , Zhang Yuebai , Gao Qing , Noman Ali , Wang Qi , Li Heng , Chen Lin , Zhou Pengyong , Lu Jing , Lou Yonggen
Published: June 2019

Plants undergo several but very precise molecular, physiological, and biochemical modulations in response to biotic stresses. Mitogen-activated protein kinase (MAPK) cascades orchestrate multiple cellular processes including plant growth and development as well as plant responses against abiotic and biotic stresses. However, the role of MAPK kinases (MAPKKs/MKKs/MEKs) in the regulation of plant resistance to herbivores has not been extensively investigated. Here, we cloned a rice MKK gene, OsMKK3, and investigated its function. It was observed that mechanical wounding, infestation of brown planthopper (BPH) Nilaparvata lugens, and treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3. The over-expression of OsMKK3 (oe-MKK3) increased levels of jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and abscisic acid (ABA), and decreased SA levels in rice after BPH attack. Additionally, the preference for feeding and oviposition, the hatching rate of BPH eggs, and BPH nymph survival rate were significantly compromised due to over-expression of OsMKK3. Besides, oe-MKK3 also augmented chlorophyll content but impaired plant growth. We confirm that MKK3 plays a pivotal role in the signaling pathway. It is proposed that OsMKK3 mediated positive regulation of rice resistance to BPH by means of herbivory-induced phytohormone dynamics.
An Engineered Hybrid Protein from Dermatophagoides pteronyssinus Allergens Shows Hypoallergenicity
Authors: Martínez Dalgys , Munera Marlon , Cantillo Jose Fernando , Wortmann Judith , Zakzuk Josefina , Keller Walter , Caraballo Luis , Puerta Leonardo
Published: June 2019

The house dust mite (HDM) Dermatophagoides pteronyssinus is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that is based on recombinant proteins has been proposed for the safer and more efficient treatment of allergic diseases. The aim of this study was to design and obtain a hybrid protein (DPx4) containing antigenic regions of allergens Der p 1, Der p 2, Der p 7, and Der p 10 from this mite. DPx4 was produced in Escherichia coli and its folding was determined by circular dichroism. Non-denaturing dot-blot, ELISA, basophil activation test, dot blot with monoclonal antibodies, ELISA inhibition, and cysteine protease activity assays were performed. Mice that were immunized with DPx4 were also analyzed. We found that DPx4 had no cysteine protease activity and it showed significantly lower IgE reactivity than Der p 1, Der p 2, and D. pteronyssinus extract. DPx4 induced lower basophil activation than Der p 2 and the allergen extract. Immunized mice produced IgG antibodies that inhibited the binding of allergic patient’s IgE to the allergen extract and induced comparatively higher levels of IL-10 than the extract in peripheral blood mononuclear cells (PBMC) culture. These results suggest that DPx4 has immunological properties that are useful for the development of a mite allergy vaccine.
IGF1R Is a Potential New Therapeutic Target for HGNET-BCOR Brain Tumor Patients
Authors: Vewinger Nadine , Huprich Sabrina , Seidmann Larissa , Russo Alexandra , Alt Francesca , Bender Hannah , Sommer Clemens , Samuel David , Lehmann Nadine , Backes Nora , Roth Lea , Harter Patrick N. , Filipski Katharina , Faber Jörg , Paret Claudia
Published: June 2019

(1) Background: The high-grade neuroepithelial tumor of the central nervous system with BCOR alteration (HGNET-BCOR) is a highly malignant tumor. Preclinical models and molecular targets are urgently required for this cancer. Previous data suggest a potential role of insulin-like growth factor (IGF) signaling in HGNET-BCOR. (2) Methods: The primary HGNET-BCOR cells PhKh1 were characterized by western blot, copy number variation, and methylation analysis and by electron microscopy. The expression of IGF2 and IGF1R was assessed by qRT-PCR. The effect of chemotherapeutics and IGF1R inhibitors on PhKh1 proliferation was tested. The phosphorylation of IGF1R and downstream molecules was assessed by western blot. (3) Results: Phkh1 cells showed a DNA methylation profile compatible with the DNA methylation class “HGNET-BCOR” and morphologic features of cellular cannibalism. IGF2 and IGF1R were highly expressed by three HGNET-BCOR tumor samples and PhKh1 cells. PhKh1 cells were particularly sensitive to vincristine, vinblastine, actinomycin D (IC50 < 10 nM for all drugs), and ceritinib (IC50 = 310 nM). Ceritinib was able to abrogate the proliferation of PhKh1 cells and blocked the phosphorylation of IGF1R and AKT. (4) Conclusion: IGF1R is as an attractive target for the development of new therapy protocols for HGNET-BCOR patients, which may include ceritinib and vinblastine.
The Role of Serine-Threonine Protein Phosphatase PP2A in Plant Oxidative Stress Signaling—Facts and Hypotheses
Authors: Máthé Csaba , Garda Tamás , Freytag Csongor , M-Hamvas Márta
Published: June 2019

Abiotic and biotic factors induce oxidative stress involving the production and scavenging of reactive oxygen species (ROS). This review is a survey of well-known and possible roles of serine-threonine protein phosphatases in plant oxidative stress signaling, with special emphasis on PP2A. ROS mediated signaling involves three interrelated pathways: (i) perception of extracellular ROS triggers signal transduction pathways, leading to DNA damage and/or the production of antioxidants; (ii) external signals induce intracellular ROS generation that triggers the relevant signaling pathways and (iii) external signals mediate protein phosphorylation dependent signaling pathway(s), leading to the expression of ROS producing enzymes like NADPH oxidases. All pathways involve inactivation of serine-threonine protein phosphatases. The metal dependent phosphatase PP2C has a negative regulatory function during ABA mediated ROS signaling. PP2A is the most abundant protein phosphatase in eukaryotic cells. Inhibitors of PP2A exert a ROS inducing activity as well and we suggest that there is a direct relationship between these two effects of drugs. We present current findings and hypotheses regarding PP2A-ROS signaling connections related to all three ROS signaling pathways and anticipate future research directions for this field. These mechanisms have implications in the understanding of stress tolerance of vascular plants, having applications regarding crop improvement.
Molecular Mechanisms and Determinants of Innovative Correction Approaches in Coagulation Factor Deficiencies
Authors: Balestra Dario , Branchini Alessio
Published: June 2019

Molecular strategies tailored to promote/correct the expression and/or processing of defective coagulation factors would represent innovative therapeutic approaches beyond standard substitutive therapy. Here, we focus on the molecular mechanisms and determinants underlying innovative approaches acting at DNA, mRNA and protein levels in inherited coagulation factor deficiencies, and in particular on: (i) gene editing approaches, which have permitted intervention at the DNA level through the specific recognition, cleavage, repair/correction or activation of target sequences, even in mutated gene contexts; (ii) the rescue of altered pre-mRNA processing through the engineering of key spliceosome components able to promote correct exon recognition and, in turn, the synthesis and secretion of functional factors, as well as the effects on the splicing of missense changes affecting exonic splicing elements; this section includes antisense oligonucleotide- or siRNA-mediated approaches to down-regulate target genes; (iii) the rescue of protein synthesis/function through the induction of ribosome readthrough targeting nonsense variants or the correction of folding defects caused by amino acid substitutions. Overall, these approaches have shown the ability to rescue the expression and/or function of potentially therapeutic levels of coagulation factors in different disease models, thus supporting further studies in the future aimed at evaluating the clinical translatability of these new strategies.
IQSEC2-Associated Intellectual Disability and Autism
Authors: Levy Nina S. , Umanah George K. E. , Rogers Eli J. , Jada Reem , Lache Orit , Levy Andrew P.
Published: June 2019

Mutations in IQSEC2 cause intellectual disability (ID), which is often accompanied by seizures and autism. A number of studies have shown that IQSEC2 is an abundant protein in excitatory synapses and plays an important role in neuronal development as well as synaptic plasticity. Here, we review neuronal IQSEC2 signaling with emphasis on those aspects likely to be involved in autism. IQSEC2 is normally bound to N-methyl-D-aspartate (NMDA)-type glutamate receptors via post synaptic density protein 95 (PSD-95). Activation of NMDA receptors results in calcium ion influx and binding to calmodulin present on the IQSEC2 IQ domain. Calcium/calmodulin induces a conformational change in IQSEC2 leading to activation of the SEC7 catalytic domain. GTP is exchanged for GDP on ADP ribosylation factor 6 (ARF6). Activated ARF6 promotes downregulation of surface α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors through a c-jun N terminal kinase (JNK)-mediated pathway. NMDA receptors, AMPA receptors, and PSD-95 are all known to be adversely affected in autism. An IQSEC2 transgenic mouse carrying a constitutively active mutation (A350V) shows autistic features and reduced levels of surface AMPA receptor subunit GluA2. Sec7 activity and AMPA receptor recycling are presented as two targets, which may respond to drug treatment in IQSEC2-associated ID and autism.
Aluminum Alters the Histology and Pectin Cell Wall Composition of Barley Roots
Authors: Jaskowiak Joanna , Kwasniewska Jolanta , Milewska-Hendel Anna , Kurczynska Ewa Urszula , Szurman-Zubrzycka Miriam , Szarejko Iwona
Published: June 2019

Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils. Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity. In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure. We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach. Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0). Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed. In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present. Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount. Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls. Additionally, histological changes were detected in the roots grown under Al exposure. Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed. The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions. The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation.
Bulky-Yet-Flexible Carbene Ligands and Their Use in Palladium Cross-Coupling
Authors: Vanden Broeck Sofie M. P. , Nahra Fady , Cazin Catherine S. J.
Published: June 2019

In recent years, several classes of new N-heterocyclic carbene (NHC) ligands were developed around the concept of “flexible steric bulk”. The steric hindrance of these ligands brings stability to the active species, while ligand flexibility still allows for the approach of the substrate. In this review, the synthesis of several types of new classes, such as IBiox, cyclic alkyl amino carbenes (CAAC), ITent, and IPr* are discussed, as well as how they move the state-of-the-art in palladium catalyzed cross-coupling forward.
Synergistic Toxicity of Plant Essential Oils Combined with Pyrethroid Insecticides against Blow Flies and the House Fly
Authors: Suwannayod Suttida , Sukontason Kabkaew L. , Pitasawat Benjawan , Junkum Anuluck , Limsopatham Kwankamol , Jones Malcolm K. , Somboon Pradya , Leksomboon Ratana , Chareonviriyaphap Theeraphap , Tawatsin Apiwat , Thavara Usavadee , Sukontason Kom
Published: June 2019

Blow flies (Diptera: Calliphoridae) and the house fly (Diptera: Muscidae) are filth flies of medical importance, and control of their population is needed. As insecticide applications have resulted in fly resistance, and the exploration of plant essential oils (EOs) has increased against filth flies, this study assessed the combination of EOs with pyrethoids to enhance toxic efficacy. The EOs of five effective plants were screened initially against the house fly (Musca domestica L.). Their chemical constituent was performed using gas chromatography-mass spectrometry (GC-MS) analysis. The main components of Boesenbergia rotunda (Zingiberaceae) rhizome, Curcuma longa (Zingiberaceae) rhizome, Citrus hystrix (Rutaceae) fruit peel, Ocimum gratissimum (Lamiaceae) seed, and Zanthoxylum limonella (Rutaceae) fruit were δ-3-caren (35.25%), β-turmerone (51.68%), β-pinene (26.56%), p-cumic aldehyde (58.21%), and dipentene (60.22%), respectively. The screening test revealed that the three most effective plant EOs were from B. rotunda, C. longa and O. gratissimum, which were selected for the combination with two pyrethroid insecticides (permethrin and deltamethrin), in order to enhance their synergistic efficacy against the blow flies, Chrysomya megacephala Fabricius, Chrysomya rufifacies Macquart, and Lucilia cuprina Wiedemann, and the house fly. Synergistic action was presented in almost all of the flies tested with permenthrin/deltamethrin/EOs mixtures. It was interesting that the combination of deltamethrin with three EOs showed a synergistic effect on all of the tested flies. However, an antagonistic effect was observed in C. megacephala and M. domestica treated with permethrin-B. rotunda and C. megacephala treated with permethrin-O. gratissimum. The LD50 of insecticides decreased when combined with plant EOs. This alternative strategy will be helpful in developing a formula for effective fly control management.
Statistical Analysis of the Mathematical Model of Entropy Generation of Magnetized Nanofluid
Authors: Abbas Munawwar Ali , Hussain Ibrahim
Published: June 2019

This investigation introduces a mathematical model of entropy generation for Magnetohydrodynamic (MHD) peristaltic wave of nanofluid. The governing equations have been created by the supposition of low Reynolds number and long wavelength estimation. The scientific arrangement has been procured with the help of perturbation technique. The concentration profile, temperature profile, pressure distribution and friction forces are shown graphically for some important parameters. Further, the eventual outcomes of connection between the entropy generation and some various parameters have been plotted by means of correlation and regression. It is fundamental to find the affectability of each parameter on entropy generation.
Non-Invasive Biomarkers for Celiac Disease
Authors: Singh Alka , Pramanik Atreyi , Acharya Pragyan , Makharia Govind K.
Published: June 2019

Once thought to be uncommon, celiac disease has now become a common disease globally. While avoidance of the gluten-containing diet is the only effective treatment so far, many new targets are being explored for the development of new drugs for its treatment. The endpoints of therapy include not only reversal of symptoms, normalization of immunological abnormalities and healing of mucosa, but also maintenance of remission of the disease by strict adherence of the gluten-free diet (GFD). There is no single gold standard test for the diagnosis of celiac disease and the diagnosis is based on the presence of a combination of characteristics including the presence of a celiac-specific antibody (anti-tissue transglutaminase antibody, anti-endomysial antibody or anti-deamidated gliadin peptide antibody) and demonstration of villous abnormalities. While the demonstration of enteropathy is an important criterion for a definite diagnosis of celiac disease, it requires endoscopic examination which is perceived as an invasive procedure. The capability of prediction of enteropathy by the presence of the high titer of anti-tissue transglutaminase antibody led to an option of making a diagnosis even without obtaining mucosal biopsies. While present day diagnostic tests are great, they, however, have certain limitations. Therefore, there is a need for biomarkers for screening of patients, prediction of enteropathy, and monitoring of patients for adherence of the gluten-free diet. Efforts are now being made to explore various biomarkers which reflect different changes that occur in the intestinal mucosa using modern day tools including transcriptomics, proteomics, and metabolomics. In the present review, we have discussed comprehensively the pros and cons of available biomarkers and also summarized the current status of emerging biomarkers for the screening, diagnosis, and monitoring of celiac disease.
Association of Low Serum Adiponectin Levels with Aortic Arterial Stiffness in Patients with Type 2 Diabetes
Authors: Shih Cian-Huei , Hsu Bang-Gee , Hou Jia-Sian , Wu Du-An , Subeq Yi-Maun
Published: June 2019

Adiponectin, an anti-inflammatory and anti-atherogenic protein, affects glucose metabolism. High serum adiponectin levels are associated with decreased diabetes mellitus (DM) risks. Aortic arterial stiffness (AS) is associated with cardiovascular disease and mortality in type 2 DM patients. We assessed the association between adiponectin levels and aortic AS in type 2 DM patients. We measured serum adiponectin levels in 140 volunteers with type 2 DM and assigned patients with carotid–femoral pulse wave velocity (cfPWV) >10 m/s to the aortic AS group (n = 54, 38.6%). These patients had higher systolic (p = 0.001) and diastolic (p = 0.010) blood pressures; body fat masses (p = 0.041); serum triglyceride (p = 0.026), phosphorus (p = 0.037), and insulin (p = 0.040) levels; and homeostasis model assessment of insulin resistance values (p = 0.029) and lower estimated glomerular filtration rates (p = 0.009) and serum adiponectin levels (p = 0.001) than controls. Multivariable logistic regression analysis adjusted for confounders showed serum adiponectin levels (OR 0.922; 95% CI, 0.876–0.970; p = 0.002) as an independent predictor of aortic AS. Multivariable forward stepwise linear regression analyses showed that serum adiponectin levels (β = −0.283, adjusted R2 change: 0.054, p < 0.001) were negatively associated with cfPWV. Thus, serum adiponectin level is an independent predictor of aortic AS in type 2 DM patients.
Fully Automated Support System for Diagnosis of Breast Cancer in Contrast-Enhanced Spectral Mammography Images
Authors: Fanizzi Annarita , Losurdo Liliana , Basile Teresa Maria A. , Bellotti Roberto , Bottigli Ubaldo , Delogu Pasquale , Diacono Domenico , Didonna Vittorio , Fausto Alfonso , Lombardi Angela , Lorusso Vito , Massafra Raffaella , Tangaro Sabina , La Forgia Daniele
Published: June 2019

Contrast-Enhanced Spectral Mammography (CESM) is a novelty instrumentation for diagnosing of breast cancer, but it can still be considered operator dependent. In this paper, we proposed a fully automatic system as a diagnostic support tool for the clinicians. For each Region Of Interest (ROI), a features set was extracted from low-energy and recombined images by using different techniques. A Random Forest classifier was trained on a selected subset of significant features by a sequential feature selection algorithm. The proposed Computer-Automated Diagnosis system is tested on 48 ROIs extracted from 53 patients referred to Istituto Tumori “Giovanni Paolo II” of Bari (Italy) from the breast cancer screening phase between March 2017 and June 2018. The present method resulted highly performing in the prediction of benign/malignant ROIs with median values of sensitivity and specificity of 87 . 5 % and 91 . 7 % , respectively. The performance was high compared to the state-of-the-art, even with a moderate/marked level of parenchymal background. Our classification model outperformed the human reader, by increasing the specificity over 8 % . Therefore, our system could represent a valid support tool for radiologists for interpreting CESM images, both reducing the false positive rate and limiting biopsies and surgeries.
Dimensional Quality and Distortion Analysis of Thin-Walled Alloy Parts of AlSi10Mg Manufactured by Selective Laser Melting
Authors: Ahmed Altaf , Majeed Arfan , Atta Zahid , Jia Guozhu
Published: June 2019

The quality and reliability in additive manufacturing is an emerging area. To ensure process quality and reliability, the influence of all process parameters and conditions needs to be understood. The product quality and reliability characteristics, i.e., dimensional accuracy, precision, repeatability, and reproducibility are mostly affected by inherent and systematic manufacturing process variations. This paper presents research on dimensional quality and distortion analysis of AlSi10Mg thin-walled parts developed by a selective laser melting technique. The input process parameters were fixed, and the impact of inherent process variation on dimensional accuracy and precision was studied. The process stability and variability were examined under repeatability and reproducibility conditions. The sample length (horizontal dimension) results revealed a 0.05 mm maximum dimensional error, 0.0197 mm repeatability, and 0.0169 mm reproducibility. Similarly, in sample height (vertical dimension) results, 0.258 mm maximum dimensional error, 0.0237 mm repeatability, and 0.0863 mm reproducibility were observed. The effect of varying design thickness on thickness accuracy was analyzed, and regression analysis performed. The maximum 0.038 mm error and 0.018 mm standard deviation was observed for the 1 mm thickness sample, which significantly decreased for sample thickness ≥2 mm. The % error decreased exponentially with increasing sample thickness. The distortion analysis was performed to explore the effect of sample thickness on part distortion. The 0.5 mm thickness sample shows a very high distortion comparatively, and it is reduced significantly for >0.5 mm thickness samples. The study is further extended to examine the effect of solution heat treatment and artificial aging on the accuracy, precision, and distortion; however, it did not improve the results. Conclusively, the sample dimensions, i.e., length and height, have shown fluctuations due to inherent process characteristics under repeatability and reproducibility conditions. The ANOVA results revealed that sample length means are not statistically significantly different, whereas sample height means are significantly different. The horizontal dimensions in the xy-plane have better accuracy and precision compared to the vertical dimension in the z-axis. The accuracy and precision increased, whereas part distortion decreased with increasing thickness.