bluuebunny/crossref_metadata_2025 huggingface dataset:
Regenerating Gene Protein as a Novel Autoantigen in the Pathogenesis of Sjögren’s Syndrome
Authors: Fujimoto Takashi , Yoshimoto Kiyomi , Fujimura Takanori , Takeda Maiko , Yamauchi Akiyo , Itaya-Hironaka Asako , Takasawa Shin
Published: December 2015

Sjögren’s syndrome, an autoimmune disease characterized by exocrine gland dysfunction leading to dry mouth and dry eye diseases, is typified by lymphoplasmacytic infiltrations and a progressive destruction of the salivary and lacrimal glands. Despite an ever-increasing focus on identifying the underlying etiology of Sjögren’s syndrome, the factors that initiate this autoimmune disease and the mechanisms that cause the subsequent exocrine gland dysfunction remain a mystery. The original explanatory concept for the pathogenesis of Sjögren’s syndrome proposed a specific, self-perpetuating, immune-mediated loss of acinar and ductal cells as the principal cause of salivary gland dysfunction. We highlight the possible involvement of regenerating gene (Reg) in the regeneration and destruction of salivary gland acinar and ductal cells in Sjögren’s syndrome. The Reg gene was originally isolated as a gene specifically overexpressed in regenerating pancreatic islets and constitutes a growth factor family (Reg family). We describe how salivary gland dysfunction is initiated and maintained and how it can be regenerated or progressed, mediated by the Reg gene, Reg protein, and anti-REG autoantibodies in Sjögren’s syndrome.
Diode-Pumped High Energy and High Average Power All-Solid-State Picosecond Amplifier Systems
Authors: Liu Jiaxing , Wang Wei , Wang Zhaohua , Lv Zhiguo , Zhang Zhiyuan , Wei Zhiyi
Published: December 2015

We present our research on the high energy picosecond laser operating at a repetition rate of 1 kHz and the high average power picosecond laser running at 100 kHz based on bulk Nd-doped crystals. With diode-pumped solid state (DPSS) hybrid amplifiers consisting of a picosecond oscillator, a regenerative amplifier, end-pumped single-pass amplifiers, and a side-pumped amplifier, an output energy of 64.8 mJ at a repetition rate of 1 kHz was achieved. An average power of 37.5 W at a repetition rate of 100 kHz pumped by continuous wave laser diodes was obtained. Compact, stable and high power DPSS laser amplifier systems with good beam qualities are excellent picosecond sources for high power optical parametric chirped pulse amplification (OPCPA) and high-efficiency laser processing.
Irradiance Impact on Pollution by Integrating Nephelometer Measurements
Authors: Perrone Maria , Burlizzi Pasquale , Romano Salvatore
Published: December 2015

Three-wavelength integrating nephelometer measurements combined with short- (SW) and long-wave (LW) irradiance measurements were used to investigate the irradiance effects on the daily evolution of the particulate matter (PM) at the ground level, and contribute to the characterization of the land–atmosphere interaction in pollution dispersal. The integrating nephelometer measurements have allowed characterizing the daily changes of the PM optical and microphysical properties by the aerosol scattering coefficient (σp) and the scattering Ångström coefficient (å). We found that on a daily basis σp reached the minimum values when the irradiance reached the maximum values, since the convective motions, which favor the particle dispersion at the surface, increase with the irradiance. The å value, which is commonly used as qualitative indicator of the dominant particle size, has allowed evaluating the irradiance effects on the mean particle size distribution at the surface and revealed that the irradiance increase favors mainly the dispersion of the ground-level fine particles. Particle size-distribution measurements supported the last comment. Measurements were performed from 4 to 10 May 2015 when the study site was affected by a Saharan dust outbreak, to also evaluate the impact of long-range transported particles on the daily evolution of the ground-level particle’s properties and the SW and LW irradiance.
The Syndrome of Catatonia
Authors: Wilcox James , Reid Duffy Pam
Published: December 2015

Catatonia is a psychomotor syndrome which has historically been associated with schizophrenia. Many clinicians have thought that the prevalence of this condition has been decreasing over the past few decades. This review reminds clinicians that catatonia is not exclusively associated with schizophrenia, and is still common in clinical practice. Many cases are related to affective disorders or are of an idiopathic nature. The illusion of reduced prevalence has been due to evolving diagnostic systems that failed to capture catatonic syndromes. This systemic error has remained unchallenged, and potentiated by the failure to perform adequate neurological evaluations and catatonia screening exams on psychiatric patients. We find that current data supports catatonic syndromes are still common, often severe and of modern clinical importance. Effective treatment is relatively easy and can greatly reduce organ failure associated with prolonged psychomotor symptoms. Prompt identification and treatment can produce a robust improvement in most cases. The ongoing prevalence of this syndrome requires that psychiatrists recognize catatonia and its presentations, the range of associated etiologies, and the import of timely treatment.
Association Studies of HFE C282Y and H63D Variants with Oral Cancer Risk and Iron Homeostasis Among Whites and Blacks
Authors: Jones Nathan , Ashmore Joseph , Lee Sang , Richie John , Lazarus Philip , Muscat Joshua
Published: December 2015

Background: Polymorphisms in the hemochromatosis (HFE) gene are associated with excessive iron absorption from the diet, and pro-oxidant effects of iron accumulation are thought to be a risk factor for several types of cancer. Methods: The C282Y (rs1800562) and H63D (rs1799945) polymorphisms were genotyped in 301 oral cancer cases and 437 controls and analyzed in relation to oral cancer risk, and serum iron biomarker levels from a subset of 130 subjects. Results: Individuals with the C282Y allele had lower total iron binding capacity (TIBC) (321.2 ± 37.2 µg/dL vs. 397.7 ± 89.0 µg/dL, p = 0.007) and higher percent transferrin saturation (22.0 ± 8.7 vs. 35.6 ± 22.9, p = 0.023) than wild type individuals. Iron and ferritin levels approached significantly higher levels for the C282Y allele (p = 0.0632 and p = 0.0588, respectively). Conclusions: Iron biomarker levels were elevated by the C282Y allele, but neither (rs1800562) nor (rs1799945) was associated with oral cancer risk in blacks and whites.
Wildfire Likelihood’s Elements: A Literature Review
Authors: Mhawej Mario , Faour Ghaleb , Adjizian-Gerard Jocelyne
Published: December 2015

Wildfires occur in different climatic zones, forest cover types and eras. Wildfire or forest fire has always shaped the landscape. Different methodologies and indexes have emerged to determine the likelihood of wildfire, commonly confused with the wildfire hazard. However, none of these are universal or portable. In this paper, we have gone through several articles, projects and books. The aim was to identify factors related to the ignition of a wildfire. Consequently, 28 factors were presented and categorized into climatic, topographic, in-situ, historical and anthropogenic factors. It is the first step in building a generalized, acceptable and portable method to determine the wildfire risk. Its creation is strongly related to the prevention and better assessment of this phenomenon.
Recent Progress in Gas Barrier Thin Film Coatings on PET Bottles in Food and Beverage Applications
Authors: Nakaya Masaki , Uedono Akira , Hotta Atsushi
Published: December 2015

This article presents a short history and the recent advancement of the development of chemical vapor deposition technologies to form thin film gas barrier coatings on PET bottles and other plastic containers in food and beverage containers. Among different gas barrier enhancement technologies, coating can show unique performance where relatively high gas barrier enhancement is possible to various gas permeants. In this article, technologically common and different points of the current thin film coating methods in this field are summarized. This article also refers to recent market situations and technological challenges in the Japanese market.
Local Stability Analysis for a Thermo-Economic Irreversible Heat Engine Model under Different Performance Regimes
Authors: Barranco-Jiménez Marco , Sánchez-Salas Norma , Reyes-Ramírez Israel
Published: December 2015

A recent work reported a local stability analysis of a thermo-economical model of an irreversible heat engine working under maximum power conditions. That work showed that after small perturbations to the working temperatures, the system decreases exponentially to the steady state characterized by two different relaxation times. This work extends the local stability analysis considering other performance regimes: the Maximum Efficient Power (MEP) and the Ecological Function (EF) regimes. The relaxation time was shown under different performance regimes as functions of the temperature ratio τ = T2/T1, with T1 > T2, the fractional fuel cost f and a lumped parameter R related to the internal irreversibilities degree. Under Maximum Efficient Power conditions the relaxation times are less than the relaxation times under both Maximum Ecological function and Maximum Power. At Maximum Power Efficient conditions, the model gives better stability conditions than for the other two regimes.
Hardware/Software Co-Design of a Traffic Sign Recognition System Using Zynq FPGAs
Authors: Han Yan , Virupakshappa Kushal , Vitor Silva Pinto Esdras , Oruklu Erdal
Published: December 2015

Traffic sign recognition (TSR), taken as an important component of an intelligent vehicle system, has been an emerging research topic in recent years. In this paper, a traffic sign detection system based on color segmentation, speeded-up robust features (SURF) detection and the k-nearest neighbor classifier is introduced. The proposed system benefits from the SURF detection algorithm, which achieves invariance to rotated, skewed and occluded signs. In addition to the accuracy and robustness issues, a TSR system should target a real-time implementation on an embedded system. Therefore, a hardware/software co-design architecture for a Zynq-7000 FPGA is presented as a major objective of this work. The sign detection operations are accelerated by programmable hardware logic that searches the potential candidates for sign classification. Sign recognition and classification uses a feature extraction and matching algorithm, which is implemented as a software component that runs on the embedded ARM CPU.
Analysis of Deep Level Defects in GaN p-i-n Diodes after Beta Particle Irradiation
Authors: Belahsene Sofiane , Al Saqri Noor , Jameel Dler , Mesli Abdelmadjid , Martinez Anthony , De Sanoit Jacques , Ougazzaden Abdallah , Salvestrini Jean , Ramdane Abderrahim , Henini Mohamed
Published: December 2015

The effect of beta particle irradiation (electron energy 0.54 MeV) on the electrical characteristics of GaN p-i-n diodes is investigated by current-voltage (I-V), capacitance-voltage (C-V) and deep-level transient spectroscopy (DLTS) measurements. The experimental studies show that, for the as-grown samples, three electron traps are found with activation energies ranging from 0.06 to 0.81 eV and concentrations ranging from 1.2 × 1014 to 3.6 × 1015 cm−3, together with one hole trap with energy depth of 0.83 eV and concentration of 8 × 1014 cm−3. It has been found that the irradiation has no effect on these intrinsic defects. The irradiation affected only a shallow donor level close to Ec [0.06 eV-0.18 eV] on the p-side of the p-i-n junction.
Cyber Risk Assessment of Transmission Lines in Smart Grids
Authors: Liu Xuan , Liu Xingdong , Li Zuyi
Published: December 2015

The increasing use of information technologies in power systems has increased the risk of power systems to cyber-attacks. In this paper, we assess the risk of transmission lines being overloaded due to cyber-based false data injection attacks. The cyber risk assessment is formulated as bilevel optimization problems that determine the maximum line flows under false data injection attacks. We propose efficient techniques to reduce the computation complexity of solving the bilevel problems. Specifically, primary and secondary filtering techniques are employed to identify the lines whose flows will never exceed their limits, which can significantly reduce computation burden. A special feasibility cut-based acceleration technique is introduced to further reduce the computation burden. The simulation results on the IEEE 30-bus, IEEE 118-bus, IEEE 300-bus and IEEE 2383-bus systems verify the proposed risk assessment model and the effectiveness of the proposed filtering and acceleration techniques.
A Lossy Counting-Based State of Charge Estimation Method and Its Application to Electric Vehicles
Authors: Zhang Hong , Zhao Li , Chen Yong
Published: December 2015

Estimating the residual capacity or state-of-charge (SoC) of commercial batteries on-line without destroying them or interrupting the power supply, is quite a challenging task for electric vehicle (EV) designers. Many Coulomb counting-based methods have been used to calculate the remaining capacity in EV batteries or other portable devices. The main disadvantages of these methods are the cumulative error and the time-varying Coulombic efficiency, which are greatly influenced by the operating state (SoC, temperature and current). To deal with this problem, we propose a lossy counting-based Coulomb counting method for estimating the available capacity or SoC. The initial capacity of the tested battery is obtained from the open circuit voltage (OCV). The charging/discharging efficiencies, used for compensating the Coulombic losses, are calculated by the lossy counting-based method. The measurement drift, resulting from the current sensor, is amended with the distorted Coulombic efficiency matrix. Simulations and experimental results show that the proposed method is both effective and convenient.
Combustion and Emission Characteristics of an LNG Engine for Heat Pumps
Authors: Lee Ziyoung , Lee Kyoungyoul , Choi Song , Park Sungwook
Published: December 2015

A gas‐engine heat pump (GHP) is driven by an internal combustion engine fueled by natural gas. The objective of this research is to model the combustion and emission characteristics of a GHP engine and experimentally validate the model. In addition, the exhaust gas recirculation (EGR) and intake boost systems were numerically investigated to find an NO and NO2 (NOx) reduction strategy. The ignition and combustion processes were modeled using the level set method and the DPIK model, and experimental data was used for validation. The models predicted engine performance and emissions for various equivalence ratios and spark timing. The study results show that late spark timing leads to reductions of the indicated specific fuel consumption (ISFC) and NOx emission, and the ISFC and NOx emissions increase with the equivalence ratio until the equivalence ratio reaches 0.9. The indicated mean effective pressure (IMEP) increases with boost pressure and decreases with increase of EGR rate. Reductions of NOx emission and high IMEP can be achieved by employing a boost system and operating at the optimal operating point, from the performance and emission map.
Gas Turbine Transient Performance Tracking Using Data Fusion Based on an Adaptive Particle Filter
Authors: Lu Feng , Wang Yafan , Huang Jinquan , Huang Yihuan
Published: December 2015

This paper considers the problem of gas turbine transient performance tracking in a cluttered environment. To increase the accuracy and robustness of state estimation, a data-fusion nonlinear estimation method based on an adaptive particle filter (PF) is proposed. This method needs local estimates transmitted to a central filtering unit for data fusion, and then global data feedback to the local PF for consensus propagation. The computational burden is shared by the local PF and central filtering unit in the data-fusion architecture. Furthermore, the PF algorithm used for the data fusion is embedded with the prior knowledge of engine health condition and adaptive to the measurement noise, and hence is called the adaptive PF. The heuristic information of state variables represented by inequality constraints tunes the local estimates by a probability density truncation method. The covariance of measurement noise is calculated by wavelet transform and utilized to update the particle importance function of the real time PF. The performance improvements of the proposed method are indicated through extensive experiments for gradual and abrupt shift performance tracking under conditions of gas turbine transient operation.
A Conceptual Study of a Supercritical CO2-Cooled Micro Modular Reactor
Authors: Yu Hwanyeal , Hartanto Donny , Moon Jangsik , Kim Yonghee
Published: December 2015

A neutronics conceptual study of a supercritical CO2-cooled micro modular reactor (MMR) has been performed in this work. The suggested MMR is an extremely compact and truck-transportable nuclear reactor. The thermal power of the MMR is 36.2 MWth and it is designed to have a 20-year lifetime without refueling. A salient feature of the MMR is that all the components including the generator are integrated in a small reactor vessel. For a minimal volume and long lifetime of the MMR core, a fast neutron spectrum is utilized in this work. To enhance neutron economy and maximize the fuel volume fraction in the core, a high-density uranium mono-nitride U15N fuel is used in the fast-spectrum MMR. Unlike the conventional supercritical CO2-cooled fast reactors, a replaceable fixed absorber (RFA) is introduced in a unique way to minimize the excess reactivity and the power peaking factor of the core. For a compact core design, the drum-type control absorber is adopted as the primary reactivity control mechanism. In this study, the neutronics analyses and depletions have been performed by using the continuous energy Monte Carlo Serpent code with the evaluated nuclear data file ENDF/B-VII.1 Library. The MMR core is characterized in view of several important safety parameters such as control system worth, fuel temperature coefficient (FTC) and coolant void reactivity (CVR), etc. In addition, a preliminary thermal-hydraulic analysis has also been performed for the hottest channel of the Korea Advanced Institute of Science and Technology (KAIST) MMR.
TOPSIS Multi-Criteria Decision Modeling Approach for Biolubricant Selection for Two-Stroke Petrol Engines
Authors: Dehghani Soufi Masoud , Ghobadian Barat , Najafi Gholamhassan , Sabzimaleki Mohammad , Yusaf Talal
Published: December 2015

Exhaust pollutants from two-stroke petrol engines are a problem for the environment. Biolubricants are a new generation of renewable and eco-friendly vegetable-based lubricants, which have attracted a lot of attention in recent years. In this paper, the applicability of the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method to support the process of building the scoring system for selecting an appropriate two-stroke lubricant has been analyzed. For this purpose, biolubricants (TMP-triesters) based on castor oil, palm oil, and waste cooking oil were produced and then utilized in a 200 cc two-stroke gasoline engine to investigate their effects on its performance and exhaust emissions. The results obtained from the use of the entropy technique in the TOPSIS algorithm showed that palm oil-based lubricant took up the greatest distance from the Negative Ideal Solution (NIS) and was selected as the most optimal lubricant for these types of engines.
Exploring Folate Diversity in Wild and Primitive Potatoes for Modern Crop Improvement
Authors: Robinson Bruce , Sathuvalli Vidyasagar , Bamberg John , Goyer Aymeric
Published: December 2015

Malnutrition is one of the world’s largest health concerns. Folate (also known as vitamin B9) is essential in the human diet, and without adequate folate intake, several serious health concerns, such as congenital birth defects and an increased risk of stroke and heart disease, can occur. Most people’s folate intake remains sub-optimal, even in countries that have a folic acid food fortification program in place. Staple crops, such as potatoes, represent an appropriate organism for biofortification through traditional breeding based on their worldwide consumption and the fact that modern cultivars only contain about 6% of the daily recommended intake of folate. To start breeding potatoes with enhanced folate content, high folate potato material must be identified. In this study, 250 individual plants from 77 accessions and 10 Solanum species were screened for their folate content using a tri-enzyme extraction and microbial assay. There was a 10-fold range of folate concentrations among individuals. Certain individuals within the species Solanum tuberosum subsp. andigenum, Solanum vernei and Solanum boliviense have the potential to produce more than double the folate concentrations of commercial cultivars, such as Russet Burbank. Our results show that tapping into the genetic diversity of potato is a promising approach to increase the folate content of this important crop.
Sleep and Cognition in Community-Dwelling Older Adults: A Review of Literature
Authors: Brewster Glenna , Varrasse Miranda , Rowe Meredeth
Published: December 2015

Changes in sleep and cognition occur with advancing age. While both may occur independently of each other, it is possible that alterations in sleep parameters may increase the risk of age-related cognitive changes. This review aimed to understand the relationship between sleep parameters (sleep latency, wake after sleep onset, sleep efficiency, sleep duration, general sleep complaints) and cognition in community-dwelling adults aged 60 years and older without sleep disorders. Systematic, computer-aided searches were conducted using multiple sleep and cognition-related search terms in PubMed, PsycINFO, and CINAHL. Twenty-nine manuscripts met the inclusion criteria. Results suggest an inconsistent relationship between sleep parameters and cognition in older adults and modifiers such as depressive symptoms, undiagnosed sleep apnea and other medical conditions may influence their association. Measures of sleep and cognition were heterogeneous. Future studies should aim to further clarify the association between sleep parameters and cognitive domains by simultaneously using both objective and subjective measures of sleep parameters. Identifying which sleep parameters to target may lead to the development of novel targets for interventions and reduce the risk of cognitive changes with aging.
Association of Vitamin A Status with Overnutrition in Children and Adolescents
Authors: Yang Chun , Chen Jing , Liu Zhen , Yun Chunfeng , Li Yajie , Piao Jianhua , Yang Xiaoguang
Published: December 2015

This study was conducted to examine the possible association between vitamin A status and overnutrition in Chinese urban children and adolescents. Weight, height and serum retinol were assessed in total 3457 children (7–9.9 years of age) and adolescents (10–17 years of age), using urban region data from the China National Nutrition and Health Survey 2010–2013 (CHNNS2010-2013) which is a nationally representative cross-sectional study. Prevalence of low serum concentration of retinol was 26.8% and 12.24% for overweight. Retinol inadequacy was significantly higher in children (32.13%) than in adolescents (24.48%). The average of retinol was significantly higher in overnutrified 42.32 μg/dL versus non-overnutrified 41.05 μg/dL (p = 0.00) children and adolescents. Overnutrified children and adolescents presented a greater chance of an increase in serum concentration of retinol (odds ratio 1.34, 95% confidence interval 1.10–1.63, and 1.48, 95% confidence interval (1.26–1.74) when compared with non-overnutrified children. An important correspondence between vitamin A deficiency and overnutrition was found. Non-overnutrified children and adolescents may have a greater chance of presenting low concentrations of retinol. Future public health strategies focused on the overnutrified population and vitamin A supplements should consider the effect of retinol on urban children and adolescents in China.
“Grandma, You Should Do It—It’s Cool” Older Adults and the Role of Family Members in Their Acceptance of Technology
Authors: Luijkx Katrien , Peek Sebastiaan , Wouters Eveline
Published: December 2015

Despite its potential, the acceptance of technology to support the ability to live independently in one’s own home, also called aging in place, is not optimal. Family members may play a key role in technology acceptance by older adults; however, it is not well understood why and how they exert influence. Based on open interviews with 53 community-dwelling older adults, this paper describes the influence of family members, including spouses, on the use of various types of consumer electronics by older adults as was reported by themselves. Such a broad focus enables understanding the use of technology as was reported by older adults, instead of its intended use. Our study reveals that the influence of each family member has its own characteristics. The influence of technology acceptance is a natural and coincidental part of the interaction with spouses and grandchildren in which entertainment and pleasure are prominent. This is also partly true for the influence of children, but their influence also is intentional and driven by concerns. Our study indicates the importance of including all family members when implementing technology in the lives of older adults. Besides information for children about the use(fullness) of devices, it is worthwhile to give grandchildren an important role, because older adults easily adopt their enthusiasm and it might eventually lighten the burden on children.
Screening for Infectious Diseases among Newly Arrived Migrants: Experiences and Practices in Non-EU Countries of the Mediterranean Basin and Black Sea
Authors: Napoli Christian , Dente Maria , Kärki Tommi , Riccardo Flavia , Rossi Pasqualino , Declich Silvia ,   Network For The Control Of Cross-border Health Threats In The Mediterranean Basin And Black Sea
Published: December 2015

Changing migration dynamics in the Mediterranean Sea and differences in infectious diseases (ID) burden between the countries of origin have raised questions whether public health actions are needed to avoid the transmission of ID. Screening newly arrived migrants for ID is one health monitoring option, offering opportunities for prevention, early detection and treatment. The authors conducted a survey among country experts in non-European Union countries of the Mediterranean and Black Sea, in order to explore current ID screening practices and policies for newly arrived migrants. The association between the existence of guidelines and the proportion of refugees in the population was also estimated. Eighteen country experts responded (90%) out of the 20 invited. Eleven countries (61%) implemented screening programmes and six (38%) had national guidelines. Screening was performed most often for tuberculosis at the holding level. A higher proportion of refugees in the population was associated with the existence of guidelines for screening (p = 0.05). Fourteen experts (88%) considered screening among migrants useful. The results show that screening for ID in newly arrived migrants is relevant for non-EU countries of the Mediterranean and Black Sea. Common guidelines could be promoted focusing on both individual and public health benefits of screening programmes.
Active Commuting Behaviors in a Nordic Metropolitan Setting in Relation to Modality, Gender, and Health Recommendations
Authors: Stigell Erik , Schantz Peter
Published: December 2015

Active commuting between home and place of work or study is often cited as an interesting source of physical activity in a public health perspective. However, knowledge about these behaviors is meager. This was therefore studied in adult active commuters (n = 1872) in Greater Stockholm, Sweden, a Nordic metropolitan setting. They received questionnaires and individually adjusted maps to draw their normal commuting route. Three different modality groups were identified in men and women: single-mode cyclists and pedestrians (those who only cycle or walk, respectively) and dual-mode commuters (those who alternately walk or cycle). Some gender differences were observed in trip distances, frequencies, and velocities. A large majority of the commuting trip durations met the minimum health recommendation of at least 10-minute-long activity bouts. The median single-mode pedestrians and dual-mode commuters met or were close to the recommended weekly physical activity levels of at least 150 minutes most of the year, whereas the single-mode cyclists did so only during spring–mid-fall. A high total number of trips per year (range of medians: 230–390) adds to the value in a health perspective. To fully grasp active commuting behaviors in future studies, both walking and cycling should be assessed over different seasons and ideally over the whole year.
Assessing Heavy Metal and PCB Exposure from Tap Water by Measuring Levels in Plasma from Sporadic Breast Cancer Patients, a Pilot Study
Authors: Zimeri Anne , Robb Sara , Hassan Sayed , Hire Rupali , Davis Melissa
Published: December 2015

Breast cancer (BrCA) is the most common cancer affecting women around the world. However, it does not arise from the same causative agent among all women. Genetic markers have been associated with heritable or familial breast cancers, which may or may not be confounded by environmental factors, whereas sporadic breast cancer cases are more likely attributable to environmental exposures. Approximately 85% of women diagnosed with BrCA have no family history of the disease. Given this overwhelming bias, more plausible etiologic mechanisms should be investigated to accurately assess a woman’s risk of acquiring breast cancer. It is known that breast cancer risk is highly influenced by exogenous environmental cues altering cancer genes either by genotoxic mechanisms (DNA mutations) or otherwise. Risk assessment should comprehensively incorporate exposures to exogenous factors that are linked to a woman’s individual susceptibility. However, the exact role that some environmental agents (EA) play in tumor formation and/or cancer gene regulation is unclear. In this pilot project, we begin a multi-disciplinary approach to investigate the intersection of environmental exposures, cancer gene response, and BrCA risk. Here, we present data that show environmental exposure to heavy metals and PCBs in drinking water, heavy metal presence in plasma of nine patients with sporadic BrCA, and Toxic Release Inventory and geological data for a metal of concern, uranium, in Northeast Georgia.
Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells
Authors: Shih Mei-Fen , Pan Kuang-Hung , Cherng Jong
Published: December 2015

Proliferation and migration of vascular smooth muscle cells (VSMC) are important in the development and/or progression of many cardiovascular diseases, including atherosclerosis. Evidence shows that matrix metalloproteinase (MMP)-2 and MMP-9 are related to the pathogenesis of atherosclerosis. The expressions of MMP-2 and MMP-9 in atherosclerosis are regulated via various pathways, such as p38 mitogen activated protein kinase (MAPK), extracellular signal regulated kinase 1 and 2 (ERK1/2), Akt, and nuclear factor kappa (NF-κB). Di(2-ethylhexyl) phthalate (DEHP) has been shown to induce atherosclerosis by increasing tumor necrosis factor (TNF)-α, interleukin (IL)-6, and intercellular adhesion molecule (ICAM) productions. However, whether DEHP poses any effects on MMP-2 or MMP-9 expression in VSMC has not yet been answered. In our studies, rat aorta VSMC was treated with DEHP (between 2 and 17.5 ppm) and p38 MAPK, ERK1/2, Akt, NF-κB, and MMP-2 and MMP-9 proteins and activities were measured. Results showed that the presence of DEHP can induce higher MMP-2 and MMP-9 expression than the controls. Similar results on MMP-regulating proteins, i.e., p38 MAPK, ERK1/2, Akt, and NF-κB, were also observed. In summary, our current results have showed that DEHP can be a potent inducer of atherosclerosis by increasing MMP-2 and MMP-9 expression at least through the regulations of p38 MAPK, ERK1/2, Akt, and NF-κB.
Angiotensin-I Converting Enzyme (ACE) Inhibitory and Anti-Oxidant Activities of Sea Cucumber (Actinopyga lecanora) Hydrolysates
Authors: Ghanbari Raheleh , Zarei Mohammad , Ebrahimpour Afshin , Abdul-Hamid Azizah , Ismail Amin , Saari Nazamid
Published: December 2015

In recent years, food protein-derived hydrolysates have received considerable attention because of their numerous health benefits. Amongst the hydrolysates, those with anti-hypertensive and anti-oxidative activities are receiving special attention as both activities can play significant roles in preventing cardiovascular diseases. The present study investigated the angiotensin-I converting enzyme (ACE) inhibitory and anti-oxidative activities of Actinopyga lecanora (A. lecanora) hydrolysates, which had been prepared by alcalase, papain, bromelain, flavourzyme, pepsin, and trypsin under their optimum conditions. The alcalase hydrolysate showed the highest ACE inhibitory activity (69.8%) after 8 h of hydrolysis while the highest anti-oxidative activities measured by 2,2-diphenyl 1-1-picrylhydrazyl radical scavenging (DPPH) (56.00%) and ferrous ion-chelating (FIC) (59.00%) methods were exhibited after 24 h and 8 h of hydrolysis, respectively. The ACE-inhibitory and anti-oxidative activities displayed dose-dependent trends, and increased with increasing protein hydrolysate concentrations. Moreover, strong positive correlations between angiotensin-I converting enzyme (ACE) inhibitory and anti-oxidative activities were also observed. This study indicates that A. lecanora hydrolysate can be exploited as a source of functional food owing to its anti-oxidant as well as anti-hypertension functions.
Use of Inotropic Agents in Treatment of Systolic Heart Failure
Authors: Tariq Sohaib , Aronow Wilbert
Published: December 2015

The most common use of inotropes is among hospitalized patients with acute decompensated heart failure, with reduced left ventricular ejection fraction and with signs of end-organ dysfunction in the setting of a low cardiac output. Inotropes can be used in patients with severe systolic heart failure awaiting heart transplant to maintain hemodynamic stability or as a bridge to decision. In cases where patients are unable to be weaned off inotropes, these agents can be used until a definite or escalated supportive therapy is planned, which can include coronary revascularization or mechanical circulatory support (intra-aortic balloon pump, extracorporeal membrane oxygenation, impella, left ventricular assist device, etc.). Use of inotropic drugs is associated with risks and adverse events. This review will discuss the use of the inotropes digoxin, dopamine, dobutamine, norepinephrine, milrinone, levosimendan, and omecamtiv mecarbil. Long-term inotropic therapy should be offered in selected patients. A detailed conversation with the patient and family shall be held, including a discussion on the risks and benefits of use of inotropes. Chronic heart failure patients awaiting heart transplants are candidates for intravenous inotropic support until the donor heart becomes available. This helps to maintain hemodynamic stability and keep the fluid status and pulmonary pressures optimized prior to the surgery. On the other hand, in patients with severe heart failure who are not candidates for advanced heart failure therapies, such as transplant and mechanical circulatory support, inotropic agents can be used for palliative therapy. Inotropes can help reduce frequency of hospitalizations and improve symptoms in these patients.
The Dipeptidyl Peptidase-4 Inhibitor Teneligliptin Attenuates Hepatic Lipogenesis via AMPK Activation in Non-Alcoholic Fatty Liver Disease Model Mice
Authors: Ideta Takayasu , Shirakami Yohei , Miyazaki Tsuneyuki , Kochi Takahiro , Sakai Hiroyasu , Moriwaki Hisataka , Shimizu Masahito
Published: December 2015

Non-alcoholic fatty liver disease (NAFLD), which is strongly associated with metabolic syndrome, is increasingly a major cause of hepatic disorder. Dipeptidyl peptidase (DPP)-4 inhibitors, anti-diabetic agents, are expected to be effective for the treatment of NAFLD. In the present study, we established a novel NAFLD model mouse using monosodium glutamate (MSG) and a high-fat diet (HFD) and investigated the effects of a DPP-4 inhibitor, teneligliptin, on the progression of NAFLD. Male MSG/HFD-treated mice were divided into two groups, one of which received teneligliptin in drinking water. Administration of MSG and HFD caused mice to develop severe fatty changes in the liver, but teneligliptin treatment improved hepatic steatosis and inflammation, as evaluated by the NAFLD activity score. Serum alanine aminotransferase and intrahepatic triglyceride levels were significantly decreased in teneligliptin-treated mice (p < 0.05). Hepatic mRNA levels of the genes involved in de novo lipogenesis were significantly downregulated by teneligliptin (p < 0.05). Moreover, teneligliptin increased hepatic expression levels of phosphorylated AMP-activated protein kinase (AMPK) protein. These findings suggest that teneligliptin attenuates lipogenesis in the liver by activating AMPK and downregulating the expression of genes involved in lipogenesis. DPP-4 inhibitors may be effective for the treatment of NAFLD and may be able to prevent its progression to non-alcoholic steatohepatitis.
Hydrolysis of Oligosaccharides by a Thermostable α-Galactosidase from Termitomyces eurrhizus
Authors: Zhang Weiwei , Du Fang , Wang Li , Zhao Liyan , Wang Hexiang , Ng Tzi
Published: December 2015

The genus of Termitomyces purchased from the market has been identified as Termitomyces eurrhizus using the Internal Transcribed Spacer (ITS) method. An α-galactosidase from T. eurrhizus (TEG), a monomeric protein with a molecular mass of 72 kDa, was purified 146 fold by employing ion exchange chromatography and gel filtration. The optimum pH and temperature was 5.0 and 60 °C, respectively. TEG was stable over pH 2–6, and also exhibited good thermostablility, retaining 100% of the original activity after incubation at 60 °C for 2 h. Inhibition of the enzyme activity by N-bromosuccinimide (NBS) constituted evidence for an essential role of tryptophan in the catalytic action of the isolated enzyme. Besides 4-nitro-phenyl α-d-galactophyranoside (pNPGal), natural substrates could also be effectively hydrolyzed by TEG. Results of thin-layer chromatography (TLC) revealed complete enzymatic hydrolysis of raffinose and stachyose to galactose at 50 °C within 6 h. These properties of TEG advocate its utilization for elevating the nutritional value of soymilk.
Intravenous Single-Dose Toxicity of Redaporfin-Based Photodynamic Therapy in Rodents
Authors: Rocha Luis , Schaberle Fábio , Dąbrowski Janusz , Simões Sérgio , Arnaut Luis
Published: December 2015

We assessed the tolerability and safety in rodents of a single intravenous (i.v.) dose of redaporfin, a novel photosensitizer for Photodynamic Therapy (PDT) of cancer. Two approaches were used to evaluate acute toxicity: (i) a dose escalation study in BALB/c mice to evaluate the maximum tolerated dose of redaporfin; and (ii) a safety toxicology study in Wistar rats, of a single dose of redaporfin, with or without illumination, to evaluate possible signs of systemic toxicity. Redaporfin formulation was well tolerated by mice, with no signs of adverse reactions up to 75 mg/kg. In rats, there were no relevant changes, except for a significant, but transient, increase in the blood serum markers for hepatic function and muscle integrity, and also on neutrophil counts, observed after the application of light. The overall results showed that redaporfin-PDT is very well tolerated. No abnormalities were observed, including reactions at the injection site or skin phototoxicity, although the animals were maintained in normal indoor lighting. Redaporfin also showed a high efficacy in the treatment of male BALB/c mice with subcutaneously implanted colon (CT26) tumours. Vascular-PDT with 1.5 mg/kg redaporfin and a light dose of 74 J/cm2 led to the complete tumour regression in 83% of the mice.
Mass Spectrometry-Based N-Glycomics of Colorectal Cancer
Authors: Sethi Manveen , Fanayan Susan
Published: December 2015

Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. An increased molecular understanding of the CRC pathology is warranted to gain insights into the underlying molecular and cellular mechanisms of the disease. Altered protein glycosylation patterns are associated with most diseases including malignant transformation. Recent advances in mass spectrometry and bioinformatics have accelerated glycomics research and present a new paradigm for cancer biomarker discovery. Mass spectrometry (MS)-based glycoproteomics and glycomics, therefore, hold considerable promise to improve the discovery of novel biomarkers with utility in disease diagnosis and therapy. This review focuses on the emerging field of glycomics to present a comprehensive review of advances in technologies and their application in studies aimed at discovering novel glycan-based biomarkers. We will also discuss some of the challenges associated with using glycans as biomarkers.
The Autonomous Glycosylation of Large DNA Viruses
Authors: Piacente Francesco , Gaglianone Matteo , Laugieri Maria , Tonetti Michela
Published: December 2015

Glycosylation of surface molecules is a key feature of several eukaryotic viruses, which use the host endoplasmic reticulum/Golgi apparatus to add carbohydrates to their nascent glycoproteins. In recent years, a newly discovered group of eukaryotic viruses, belonging to the Nucleo-Cytoplasmic Large DNA Virus (NCLDV) group, was shown to have several features that are typical of cellular organisms, including the presence of components of the glycosylation machinery. Starting from initial observations with the chlorovirus PBCV-1, enzymes for glycan biosynthesis have been later identified in other viruses; in particular in members of the Mimiviridae family. They include both the glycosyltransferases and other carbohydrate-modifying enzymes and the pathways for the biosynthesis of the rare monosaccharides that are found in the viral glycan structures. These findings, together with genome analysis of the newly-identified giant DNA viruses, indicate that the presence of glycogenes is widespread in several NCLDV families. The identification of autonomous viral glycosylation machinery leads to many questions about the origin of these pathways, the mechanisms of glycan production, and eventually their function in the viral replication cycle. The scope of this review is to highlight some of the recent results that have been obtained on the glycosylation systems of the large DNA viruses, with a special focus on the enzymes involved in nucleotide-sugar production.
Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells
Authors: Huang Wen-Shih , Yang Jen-Tsung , Lu Chien-Chang , Chang Shun-Fu , Chen Cheng-Nan , Su Yu-Ping , Lee Ko-Chao
Published: December 2015

A high level of serum resistin has recently been found in patients with a number of cancers, including colorectal cancer (CRC). Hence, resistin may play a role in CRC development. Fulvic acid (FA), a class of humic substances, possesses pharmacological properties. However, the effect of FA on cancer pathophysiology remains unclear. The aim of this study was to investigate the effect of resistin on the endothelial adhesion of CRC and to determine whether FA elicits an antagonistic mechanism to neutralize this resistin effect. Human HCT-116 (p53-negative) and SW-48 (p53-positive) CRC cells and human umbilical vein endothelial cells (HUVECs) were used in the experiments. Treatment of both HCT-116 and SW-48 cells with resistin increases the adhesion of both cells to HUVECs. This result indicated that p53 may not regulate this resistin effect. A mechanistic study in HCT-116 cells further showed that this resistin effect occurs via the activation of NF-κB and the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Co-treating cells with both FA and resistin revealed that FA significantly attenuated the resistin-increased NF-κB activation and ICAM-1/VCAM-1 expression and the consequent adhesion of HCT-116 cells to HUVECs. These results demonstrate the role of resistin in promoting HCT-116 cell adhesion to HUVECs and indicate that FA might be a potential candidate for the inhibition of the endothelial adhesion of CRC in response to resistin.
Relevance of Rabbit VX2 Tumor Model for Studies on Human Hepatocellular Carcinoma: A MicroRNA-Based Study
Authors: Aravalli Rajagopal , Cressman Erik
Published: December 2015

MicroRNAs are small (~22 nt), noncoding RNA molecules that have critical cellular functions in proliferation, differentiation, angiogenesis and apoptosis. miRNA expression profiling has been used to create signatures of solid tumors and, in many cases, it has been shown to correlate with the severity of the disease. The rabbit VX2 tumor model has been used widely to study a number of human cancers. Our objective in this study is to generate an miRNA signature of the VX2 tumor and to identify miRNAs that are highly expressed in this aggressive tumor. In this study, we performed miRNA profiling of the rabbit VX2 tumor using a microarray that has probes for 1292 unique miRNAs. Their expression in tumor samples was quantified and analyzed. We found that 35 miRNAs were significantly up-regulated in the VX2 tumor. Among these, 13 human miRNAs and eight members of the let-7 family were previously identified in cancers. In addition, we show that the expression of three miRNAs (miR-923, miR-1275, and miR-1308) is novel for the rabbit VX2 tumor, and their expression was not previously shown to be associated with any type of cancer. For the first time, we show the miRNA signature profile for a solid tumor in a rabbit model. miRNAs highly expressed in the VX2 tumor may serve as novel candidates for molecular biomarkers and as potential drug targets.
The Role of Citizen Science in Landscape and Seascape Approaches to Integrating Conservation and Development
Authors: Sayer Jeffrey , Margules Chris , Bohnet Iris , Boedhihartono Agni , Pierce Ray , Dale Allan , Andrews Kate
Published: December 2015

Initiatives to manage landscapes for both biodiversity protection and sustainable development commonly employ participatory methods to exploit the knowledge of citizens. We review five examples of citizen groups engaging with landscape scale conservation initiatives to contribute their knowledge, collect data for monitoring programs, study systems to detect patterns, and test hypotheses on aspects of landscape dynamics. Three are from landscape interventions that deliberately target biodiversity conservation and aim to have sustainable development as a collateral outcome. The other two are driven primarily by concerns for agricultural sustainability with biodiversity conservation as a collateral outcome. All five include programs in which, management agencies support data collection by citizen groups to monitor landscape changes. Situations where citizen groups self-organise to collect data and interpret data to aid in landscape scale decision making are less common and are restricted to landscapes where the inhabitants have a high level of scientific literacy. Given the complexity of landscape processes and the multiple decision makers who influence landscape outcomes we argue that citizen science broadly defined should be an essential element of landscape scale initiatives. Conservation managers should create space for citizen engagement in science and should empower citizen groups to experiment, learn, and adapt their decision-making to improve landscape scale outcomes.