bluuebunny/crossref_metadata_2025 huggingface dataset:
ОРГАНИЗАЦИЯ В РЕСПУБЛИКЕ БЕЛАРУСЬ КЛАСТЕРА ИСПОЛЬЗОВАНИЯ ВТОРИЧНЫХ МОЛОЧНЫХ РЕСУРСОВ
Authors: БАХМАТОВА Е.
Published: August 2021

В статье рассматриваются возможности реализации в Республике Беларусь кластерного подхода в сочетании с централизацией производственных мощностей по переработке вторичных молочных ресурсов. Определены институциональные особенности организации кластера использования вторичных молочных ресурсов, а также ключевые роли государственных органов управления в стимулировании кластерной инициативы в обозначенном направлении.
ПРИЧИНЫ ПЕРЕХОДА К НОВОМУ ТЕХНОЛОГИЧЕСКОМУ УКЛАДУ
Authors: НАУМОВИЧ О.
Published: August 2021

Рассмотрены некоторые причины смены технологических укладов в экономике Республики Беларусь, выявлена их взаимообусловленность. В процессе исследования изучены предпосылки, а также последствия смены технологического уклада.
КОНЦЕПТУАЛЬНЫЕ ПОДХОДЫ К ОЦЕНКЕ РОЛИ И ЗНАЧЕНИЯ УПРАВЛЕНЧЕСКОГО УЧЕТА В СИСТЕМЕ ЭНЕРГЕТИЧЕСКОГО МЕНЕДЖМЕНТА
Authors: САМОСЮК Н.
Published: August 2021

В статье автор рассматривает концептуальные подходы и особенности использования управленческого учета в системе менеджмента организации. Выделяет общие черты и отличия финансового и управленческого учетов. Обосновывает ведение раздельного учета затрат по видам экономической деятельности в энергетике Республики Беларусь. Предлагает авторскую модель управленческого учета на предприятиях энергетики Республики Беларусь.
Experimental Study on the Permeability of Quartz Sandstone under Coupled Thermo-Hydromechanical Loading
Authors: Zhang Yu , Yu Tingting , Li Jianwei , Jia Yun , Li Dayong
Published: August 2021

This paper focuses on the influence of coupled thermo-hydromechanical processes on the permeability of quartz sandstone. The permeability has been studied under five different confining pressures, three different temperatures, and three fluid pressures. The experimental results exhibit that the permeability of quartz sandstone decreases with the increase of confining pressure while it increases with temperature and fluid pressure. The identification of permeability under fully coupled thermo-hydromechanical conditions is also studied via the realization of four coupled tests. One observes that the temperature plays a more important role on the permeability with respect to the fluid pressure. Moreover, the influence of fluid pressure on the permeability of studied sandstone has been amplified by the temperature. The obtained experimental results allow us to get a good understanding of the permeability of quartz sandstone in petroleum engineering and can help us to guarantee the long-term structural stability.
Avances en investigación sobre evaluación del efecto de metotrexato y placebo en ensayos clínicos, en la progresión radiológica de artritis reumatoidea, medida por resonancia magnética nuclear: un metanálisis
Authors:   , Solano Loza Carola Andrea
Published: August 2021

La artritis reumatoide por lo general se presenta en personas de edad entre 20 y 30 años, y en el Perú más del 1 por ciento de la población padece de esta enfermedad, y cada año se pronostican unos 100 nuevos casos según la Sociedad Peruana de Reumatología. La Resonancia Magnética Nuclear – RMN – es una prueba que se maneja para evidenciar irregularidades en el tejido blando de las articulaciones, no sensibles a los rayos X, con lo cual se tiene un resultado que permite evidenciar la existencia de mayores desordenes debido a la enfermedad o si el procedimiento de tratamiento es positivo. Sin embargo, es posible evaluar la progresión radiológica medida por RMN en donde se haya estudiado el tratamiento de artritis reumatoide con una rama activa versus una rama metrotexate más placebo, para ver si existe diferencia significativa entre el compromiso articular a largo plazo entre ambos grupos de estudio. Según la Sociedad Radiológica de los Estados Unidos de Norteamérica, la aplicación de una RMN permite lograr perfiles detallados del cuerpo o de una sección del mismo. El espectro que se logra es sumamente amplio ya que permite visualizar no solo los huesos sino también lo tejidos, ligamentos y revestimientos de tejido interiores y exteriores a las articulaciones. Al llevar a cabo una investigación que examine la eficacia de una droga modificadora de la enfermedad reumatoidea (DMARD) o medicación biológica para el estudio de pacientes con artritis reumatoide, es necesario ubicar un grupo de pacientes que se encuentren recibiendo la droga activa y otro grupo que recibirá placebo. Por lo general estos estudios se catalogan como doble ciego, multicéntricos, de manera que, excepto casos muy aislados, ni el paciente, ni el médico tratante conocerán en qué rama farmacológica se encuentra el paciente.
El proceso y tiempo de atención del triaje, y su influencia en la satisfacción de los pacientes de emergencia de la Clínica Vesalio, Lima 2020
Authors:   , Ramírez Poggi Max Dante , Ernaú Venegas Erick Arthur
Published: August 2021

Objetivo: Analizar cómo el proceso y tiempo de atención del triaje Influye en la satisfacción de los pacientes de emergencia de la Clínica Vesalio, Lima 2020. Materiales y métodos: Se trató de una investigación no experimental, de corte transversal, con enfoque cuantitativo, de diseño descriptivo, y nivel correlacional. Con una muestra de 166 pacientes atendido en la emergencia de la Clínica Vesalio 2020, a quienes se les aplicó un cuestionario. Resultados: El 84,9% de los pacientes entrevistados que, asisten a la Clínica Vesalio expresaron que, no les explicaron el proceso del triaje. Para el 52,4% de los entrevistados, el personal de la clínica no los orientó a identificar el área de la emergencia donde se va realizar la atención. Por otro lado, según el 85,5%, no recibieron atención mientras esperaban el diagnóstico médico. El 19.3% se encuentra de manera regular en relación a las mejoras y soluciones ofrecidas al problema de salud por el cual fueron atendidos. El 1,2% y el 3,6% de los pacientes entrevistados expresaron estar muy insatisfechos e insatisfechos con respecto a la atención inmediata a la llegada de la emergencia. El 15,1% indicaron que la atención es regular. El 5,4% de los entrevistados, expresaron estar insatisfechos, con la rapidez en la atención ofrecida en la farmacia. El 16,3% indicó que la rapidez en la atención ofrecida es regular. Conclusiones: El proceso y tiempo de atención del triaje, se encuentra relacionado con la satisfacción de los pacientes de emergencia de la Clínica Vesalio.
Barreras y facilitadores en la implementación de guías de práctica clínica en México: perspectiva del personal de salud
Authors: Maya-Hernández Cynthya , Flores-Hernández Sergio , Vértiz-Ramírez José de Jesús , Ruelas-González María Guadalupe , Poblano-Verástegui Ofelia , Saturno-Hernández Pedro Jesús
Published: August 2021

Objetivo. Identificar barreras, facilitadores y propuestas de mejora en la implementación de Guías de Práctica Clínica (GPC) desde la perspectiva de los profesionales de la salud. Material y métodos. Estudio cualitativo a través de 85 entrevistas semiestructuradas a responsables de implementa­ción, difusión y aplicación, y del personal operativo en centros de atención primaria y hospitales en siete estados de México. El contenido fue codificado y analizado con ATLAS.ti 7.0. Resultados. Las principales barreras encontradas fueron la no actualización de las GPC y baja alineación con otras normas, e imposibilidad de implementarlas debido a la sobre­carga de trabajo y los recursos limitados. Conclusiones. El esfuerzo por implementar GPC parece haber sido errático e insuficiente, y la evaluación de su utilización inexistente. Se propone crear estrategias integradas y contextualizadas que resulten ser más efectivas y eficientes para la implementación exitosa de GPC.
Foam Flow in Different Pore Systems—Part 2: The Roles of Pore Attributes on the Limiting Capillary Pressure, Trapping Coefficient, and Relative Permeability of Foamed Gas
Authors: Adebayo Abdulrauf Rasheed
Published: August 2021

The limiting capillary pressure of foam (Pc*) and foam trapping in porous media are pore-scale foam properties that affect foam transport in porous media. They are strongly influenced by the characteristics of rock pores and throats. Because of experimental limitations, these foam properties are difficult to measure at core scale. As a result, our understanding of their relationship with different pore characteristics is limited. In this paper, novel coreflood and graphical analysis techniques were used to measure Pc* and the foam-trapping coefficient (FTC) at core scale. FTC is a new parameter synonymous to Land’s (1968) trapping coefficient, which describes foam-trapping behavior across an entire range of saturation as opposed to a single endpoint trapped saturation. The scalability of these two foam properties with permeability and other pore characteristics [average pore size (PS), average throat size (TS), average aspect ratio (AR), coordination number (CN), surface area/volume ratio, and reservoir-quality index (RQI)] were also investigated. Pore characteristics of 12 different rock samples were measured from 3D pore-network models generated from high-resolution X-ray computed-microtomography images. The heterogeneity of the rock samples were quantified by the Dykstra-Parsons index (Dysktra and Parsons 1950), while the RQI and J-function methods were used to classify them according to their storage and flow properties. Each of the measured pore characteristics and their combination [combined pore character (CPC)] were then correlated with Pc* and FTC to understand their respective roles. Furthermore, the data points obtained from the graphical analysis of the coreflood data provided the required input data for a mechanistic foam model for relative permeability of foamed gas (Kovscek and Radke 1994). The estimated relative permeability of foamed gas was then used to study foam mobility in the different pore geometries.

The overall results showed the following:

P c * has strong negative correlations with all pore characteristics except AR, which has a weak positive correlation. P c * has the strongest correlation with RQI, CPC, and permeability; a moderate correlation with CN and TS; and a very weak correlation with PS. Foam trapping has positive correlations with all pore characteristics except AR, which has a negative correlation. Low AR appears to be responsible for significant trapping of foam in high-permeability rocks. Low AR favors more foam trapping, while high AR favors trapping of oil and gas during water imbibition in water-wet rocks. Foam trapping appears to have the dominant control on foam mobility.
FRET-Seq: a High-Throughput FRET-Based Screening Platform to Improve FRET Biosensors in Mammalian Cells
Authors: Liu Longwei , Limsakul Praopim , Meng Xianhui , Huang Yan , Harrison Reed E. S. , Huang Tse-Shun , Shi Yiwen , Yu Yiyan , Charupanit Krit , Zhong Sheng , Lu Shaoying , Zhang Jin , Chien Shu , Sun Jie , Wang Yingxiao
Published: August 2021

Genetically-encoded biosensors based on FRET have been widely used to dynamically monitor the activity of protein tyrosine kinases (PTKs) in living cell with high spatiotemporal resolution. However, the limitation in sensitivity, specificity, and dynamic range of FRET biosensors have hindered their broader applications. Here, we introduced a systematic platform, FRET-Seq, which integrates high-throughput FRET sorting and next-generation sequencing, to identify FRET biosensors with better performance from large-scale libraries directly in mammalian cells.
MED29, A Subunit of the Mediator Complex, Possesses Oncogenic Characteristics in Ovarian Cancer
Authors: Wu Haifang , Chen Ling , Wu Yiguo , Lou Weiming , Nie Penghui , Tang Xiaoli , Fu Fen
Published: August 2021

Background: Somatic copy number alteration (SCNA) usually accompanies the appearance of tumours; one common example is the 19q13.2 region amplification (AMP), mediator complex subunit 29 (MED29) were an amplified effector gene within this region. In this study, the role and molecular mechanism of MED29 in ovarian cancer (OvCa), one of the three major tumours in gynaecology, were discussed.Results: According to the transcriptome and survival data from the TCGA database, 19q13.2 AMP corresponded with a worse prognosis of OvCa patients (P = 0.0253), and individuals with 19q13.2 AMP showed increased levels of MED29 mRNA. From the GSE29450 dataset, we found that the gene expression of MED29 was significantly upregulated in OvCa and overexpression of MED29 in OvCa was related to shorter survival. Additionally, pathway analysis based on RNA-seq data indicated that MED29 could activate the expression of genes involved in cell proliferation. Moreover, knockdown of MED29 obvious inhibited the proliferation, invasion and migration of OVCAR3 and A2780 cells and arrested the cell cycle at the G2/M transition in vitro.Conclusion: MED29 could be used as a potential therapeutic target for OvCa treatment.
Can Internet Use Improve Farmers' Welfare Effect——A Case Study of Chinese Vegetable Growers
Authors: Yan Di , Xu Ge
Published: August 2021

This article uses the data of 797 vegetable growers in Shouguang, Shandong Province, and the endogenous conversion model to investigate the impact of Internet use on household welfare. We select the per-mu vegetable yield, net income, and per capita net income of households as welfare indicators. The results show: ① Internet use can significantly improve the farmers' welfare effect. ②Under counterfactual assumptions, when farmers who use the Internet do not use it, the farmers' vegetable yield per mu, net income and household per capita net income will drop by 10.88%, 13.96% and 9.46%. When farmers who do not use the Internet use it, the farmers' vegetable output, net income and family per capita net income will rise by 13.62%, 16.66% and 11.64%. Internet use has the most excellent effect on the net income of vegetables, followed by the yield per mu, and the net income per household is the lowest. ③ Compared with small-scale farmers, Internet use has a better impact on the welfare of large-scale farmers, which also widens the welfare gap between farmers to a certain extent. Based on this, make suggestions to strengthen information infrastructure, improve information technology training, and adjust support policies promptly.
Silibinin can enhance the osseointegration of titanium rods in ovariectomized rats maintenance treatment with valproic acid
Authors: tao zhoushan
Published: August 2021

The present work was aimed to evaluate the effect of valproic acid(VPA)，Silibinin (SIM)+VPA on Ti rods osseointegration in ovariectomized rats and further investigation of the possible mechanism. The MC3T3-E1 cells were co-cultured with VPA，SIM +VPA and induced to osteogenesis, and the cell viability，mineralization ability were observed by MTT and ALP staining，Alizarin Red staining and Western blotting. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group OVX and VPA，SIM +VPA, and all the rats received Ti implants and animals belong to group VPA，SIM +VPA received valproic acid(300 mg/kg/day), valproic acid(300 mg/kg/day) plus SIM (25mg/kg/day) , respectively, treatment until death at 12 weeks. Micro-CT, histology, biomechanical testing, bone metabolism index and Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis were used to observe the therapeutic effect and explore the possible mechanism. Results shown that VPA decreased new bone formation around the surface of titanium rods and push-out force other than group OVX. Histology, Micro-CT and biochemical analysis results showed combined application of systemic VPA showed harmful effects than OVX group on bone formation in osteopenic rats, with the worse effects on CTX-1, P1NP and microarchitecture as well as biomechanical parameters by down-regulated gene expression of Runx2, OCN, Smad1, BMP-2 and OPG, while up-regulated RANKL. However, after SIM treatment, the above indicators were significantly improved. The present study suggests that systemic use of VPA may bring harm to the stability of titanium implants in osteoporosis, SIM can reverse the negative effect of VPA on the osseointegration of titanium rods in ovariectomized rats.
Development of a Social Distancing Monitoring System in Korea: Results of a Modified Delphi Process
Authors: Seo Suin , Han Jin-ok , Shin Sol , Lee Heeyoung
Published: August 2021

Background: Since the COVID-19 pandemic is an ongoing situation in most countries worldwide, a “social distancing” policy as a non-pharmaceutical intervention has been implemented for several months in many countries including Korea. Social distancing policies work in different ways and at different levels. In addition, various forms of surveillance systems have been implemented in different countries. However, there is an almost complete lack of specific surveillance system in Korea to effectively monitor social distancing policy. This study aims to develop a monitoring system for social distancing measures in Korea to evaluate and improve the implemented policy. Methods: A draft monitoring system was developed after reviewing Korea's social distancing measures (central and local government briefings) and checking available data for applications. The modified Delphi process was used to evaluate the draft of the monitoring system. In total, 27 experts participated in the evaluation. The round 1 evaluation includes (1) commenting on the composition of the monitoring fields (open response), (2) monitoring indicators for each monitoring field (10-point Likert scale), and (3) commenting on the source of data used to develop the monitoring system (open response). In the round 2 evaluation, 55 indicators, excepting open responses, were re-evaluated.Results: The response rate for the Delphi survey was 100% in both the first and second rounds. Of the 55 indicators, 14 were excluded according to experts’ open response comments, as these indicators did not satisfy the quantitative criteria. Finally, 41 indicators were included with 12 available data sources. The monitoring system domain was divided into input, process/output, and result.Conclusions: This study is significant in that it is the first in Korea to develop a comprehensive monitoring system for social distancing policy, and is applicable to estimates utilizing data that are immediately available for each indicator. Furthermore, the developed monitoring system could be a reference for other countries that require the development of such systems to monitor social distancing measures.
Biomechanical Modeling of the Pelvic System: Improving the Accuracy of Prostate Lesions Location in MRI–TRUS Fusion
Authors: Qasim Muhammad , Puigjaner Dolors , Herrero Joan , López Josep M. , Garcia–Bennett Josep R. , Olivé Carme , Fortuny Gerard
Published: August 2021

Background: An accurate knowledge of prostate lesions relocation during biopsy is of great importance to prevent mispositioning of the needle and thus reduce the chance of faulty biopsy results (false negatives). Prostate lesions are visible in magnetic resonance images (MRI) but it is difficult for the practitioner to locate them at the time of performing a transrectal ultrasound (TRUS) guided biopsy. In this study, we present a new methodology that predicts both prostate deformation and lesion migration during the biopsy.Methods: A thee-dimensional (3-D) anatomy model of the pelvic region, based on medical images, is constructed. A finite element (FE) simulation of the organs motion and deformation as a result of the pressure exerted by the TRUS probe was carried out using the code_aster open source software. Initial positions of potential prostate lesions prior to biopsy are taken into consideration and the final location of each lesion is targeted in the FE simulation output.Results: Our 3-D FE simulations show that the effect of the pressure exerted by the TRUS probe is twofold as the prostate experiences both a motion (in the absolute frame of reference) and a deformation of its original shape. We targeted the relocation of five small prostate lesions when the TRUS probe exerts a force of 30 N on the rectum inner wall. The distance traveled by these lesions ranged between 5.6 and 13.9 mm. Moreover, we showed that as a result of the deformation of the prostate the resulting lesion migration might be very difficult to predict if the biopsy practitioner has to rely exclusively on a visual comparison of axial or sagittal prostate slices taken from MRI and TRUS images.Conclusions: The preliminary results presented here show that our new methodology can be of great help for improving the prediction of cancerous lesions location when a TRUS guided biopsy is performed. Moreover, the new methodology is completely developed on open source software, which means that its implementation would be affordable even for healthcare providers with small budgets.
Updated Cutoff Values of Basal Serum Calcitonin to Discriminate True Medullary Thyroid Cancer from Other Conditions of Hypercalcitoninemia
Authors: Liu Qianhui , Nie Xin , He Yong , Li Guixing
Published: August 2021

Background

Calcitonin (Ctn) is a tumor marker of medullary thyroid carcinoma (MTC). However, serum Ctn cutoff values for MTC diagnosis are still under discussion. This study aimed to identify the cutoff values of Ctn and explore the relationship between two tumor markers (Ctn and carcinoembryonic antigen (CEA)) and disease burden.
Methods

This was a retrospective study conducted in West China Hospital of Sichuan University. We included 61 MTC patients and 235 non-MTC patients with nonspecific elevation of Ctn (> 9.52 pg/mL for males and > 6.40 pg/mL for females) and collected patients’ demographic information, essential serological indicators, cervical ultrasound and pathological reports.
Results

Unlike MTC, papillary thyroid carcinoma (40.85%), uremia (18.73%), chronic renal disease (10.21%) and inflammation (8.94%) commonly occurred with hypercalcitoninemia. The Ctn cutoff values were 38.24 pg/mL for males and 26.00 pg/mL for females. The Ctn level was found to be positively related to the largest tumor diameter (r = 0.702). Serum Ctn levels were significantly higher in patients with lymph node metastasis than in those without (P < 0.05), but CEA levels did not differ (P > 0.05).
Conclusion

The best Ctn cutoff values for Chinese people to discriminate MTC from other hypercalcitoninemia conditions are 38.24 pg/mL for males and 26.00 pg/mL for females.
Risk Factors for the Morbidity and Prognosis of Lung Metastases in Newly Diagnosed Ovarian Carcinoma: A Large Population-based Study
Authors: Ding Shuning , Guo Kaibo , Wu Linqin , Li Dongxu , Wang Peipei , Sun Leitao , Ruan Shanming , Tao Fangfang
Published: August 2021

Background: To evaluate the risk factors for the morbidity and prognosis of lung metastases (LM) in patients with newly diagnosed ovarian carcinoma (OC). Methods: Based on the Surveillance, Epidemiology, and End Results (SEER) dataset, OC patients from 2010 and 2016 were retrospectively analyzed. Risk factors for the morbidity of LM in OC patients and their survival were assessed by logistic regression analysis and Kaplan-Meier and Gray method, respectively. Cox regression analysis was performed to identify risk factors for the prognosis of OC patients with LM, and their prognostic potentials were further validated by two established nomograms.Results: There are 27,123 eligible OC patients were enrolled in the study, with the morbidity of LM at 5.61% (1,521/27,123). Logistic regression models illustrated that T3 stage [odds ratio (OR)=2.74, 95%CI=2.09-3.66, P<0.01], advanced N stage (OR=1.86, 95%CI=1.62-2.14, P<0.01), and the prevalence of bone metastasis (OR=3.78, 95%CI=2.79-5.11, P<0.01), brain metastasis (OR=4.67, 95%CI=2.50-8.63, P<0.01) and liver metastasis (OR=3.60, 95%CI=3.14-4.12, P<0.01) were all significantly correlated with the morbidity of LM in OC patients. Median survival for OC patients with LM was 11 months (interquartile range, 3 to 25 months). Cox regression analyses illustrated over 80 years of age [hazard ratio (HR)=2.52, 95%CI=2.33-2.72, P<0.01] and positive expression of cancer antigen 125 (CA-125, HR=1.63, 95%CI=1.47-1.82, P<0.01) were significantly correlated with the high mortality of LM, while chemotherapy (HR=0.62, 95%CI=0.59-0.65, P<0.01) was significantly correlated with the low mortality. Two nomograms were established to examine the concordance index (C-index), calibration curves, the area under the curve (AUC), decision curve analyses (DCAs) and clinical impact curves (CICs), which validated the prognostic potentials of identified risk factors in OC patients with LM. Conclusion: The population-based cohort study provides references for guiding clinical screening and individualized treatment of OC patients with LM.
Genome-Wide Study of the ABI3 Gene Family and Identification of Putative miRNA Targeting ABI3 Gene in Oryza Sativa ssp. Indica
Authors: Vashisth Apoorva , Singh Deepak Kumar , Chakraborty Navjyoti , Purty Ram Singh , Chatterjee Sayan
Published: August 2021

Rice is one of the important cereal crops mainly cultivated in Asia and its productivity is severely affected by drought stress. In response to drought stress, several genes are reported to be up-regulated or down-regulated in plants. Gene expression is negatively regulated by non-coding endogenous microRNAs post-transcriptionally either by mRNA degrading or translational silencing. In the past, single or multiple stress-responsive genes were over-expressed in order to generate drought-tolerant transgenic rice but with very little success. Recently, the development of transgenic plants by over-expressing transcription factors have received much attention because of their ability to regulate several genes. Abscisic Acid Insensitive 3 (ABI3) is a transcription factor, which is known to play a crucial role in mediating plant stress tolerance. Using the Ensembl plants database, we identified 83 putative OsABI3 genes in Oryza sativa Indica. Through in silico approach, five potential miRNAs i.e., ath-miR5021, csi-miR3948, osa-miRf11773-akr, osa-miRf12029-akr and ptc-miRf10053-akr that target OsABI3 genes were identified. Further, the expression of the selected ABI3 genes were analyzed in rice seedlings exposed to 15% PEG, using the RT-qPCR. In comparison to control, OsABI3 genes showed relatively enhanced expression when exposed to drought stress treatment. This indicates that OsABI3 genes may play important role in development and drought stress in rice seedlings.
Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers
Authors: Parab Ankita Rajendra , Lynn Chew Bee , Subramaniam Sreeramanan
Published: August 2021

In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.
Tensile behavior of a Brazilian Disk Containing non-persistent Joint Sets Subjected to Diametral Loading: An Experimental Investigation
Authors: Asadizadeh Mostafa , Shakeri Jamshid , Babanouri Nima , Rezaei Mohammad
Published: August 2021

Structural defects are part of the inherent characteristics of rock masses. They can be found in the form of fishers, joints, and beddings and can be divided into persistent or non-persistent one. The coalescence of non-persistent cracks may lead to the formation of persistent joints under the tensile stress field, leading to instability of rock mass. The mechanical behavior of non-persistent jointed disks under tensile stress has essential implications for rock engineering structures. In this paper, concrete Brazilian disks containing open non-persistent joints were constructed and subjected to diametral loading to investigate the effect of this kind of joint parameters on the tensile strength and stiffness of disks. The effect of some parameters, such as joint continuity factor (the relationship between joint length and rock bridge length), bridge angle, joint spacing, and loading direction with respect to joint angle were investigated to estimate the tensile strength and stiffness as well as failure pattern. The results of experiments revealed that the tensile strength, stiffness, and failure pattern of Brazilian disks are highly affected by non-persistent pre-existing crack parameters. The increase of joint continuity factor and loading direction leads to an increase in tensile strength and a decrease in stiffness. However, when bridge angle and spacing increase tensile strength rises, and the former decreases stiffness while the latter results in its reduction. Finally, all the parameters significantly affect the failure pattern, and some failure patterns such as step-path failure, splitting, or sliding may occur as a function of non-persistent joint parameters.
Immunohistochemical Characterization of Cell of Origin in Diffuse Large B-cell Lymphoma and its Correlation with the Double Expressor Phenotype: A Retrospective Study in a Tertiary Cancer Care Centre
Authors: Chakrabarti suparno , aiyer Hema MALINI , sharma gaurav , jaiswal sarita rani , prachi Prachi
Published: August 2021

Background – Diffuse Large B Cell Lymphoma (DLBCL) is the most common type of Non- Hodgkin Lymphoma (NHL) and is categorized into the Germinal Centre B-cell (GCB) and Activated B-cell (ABC/ Non-GCB) subtypes as per the Cell-of-origin (COO) model with the help of Gene Expression Profiling/ Immunohistochemistry. The non -GCB subtype has been found to correlate with the Double Expressor (DE) phenotype (i.e. co-expression of bcl-2 and c-myc by IHC) and this correlation was substantiated and proven to be statistically significant in the present study.Results- A retrospective study of 50 patients was carried out with the help of archival material filed in the Department of Anatomic Pathology at Dharamshila Narayana Superspeciality Hospital from 1st January 2019 to 30th June 2020. The study cohort was divided into two Groups – Group-A with nodal presentation and Group -B with extranodal presentation. By using the Hans and Choi IHC algorithms, the cases were categorized into the GCB and non – GCB subtypes in both the groups. The DE phenotype was determined in each case by the co-expression of c-myc (>40%) and bcl-2 (>50%) by IHC.Conclusion – Due to the heterogeneity inherent in DLBCL, prediction of the Double Expressor phenotype and the COO by IHC is a sensitive tool and helps in the prognostication and therapeutic triage of patients. Hence in all cases diagnosed as DLBCL, a detailed morphological and IHC work up is mandatory to determine prognosis and possibly tailor therapy according to cell of origin and double expressor phenotype.
High Rate of Epidermal Growth Factor Receptor-Mutated Primary Lung Cancer in Patients with Primary Breast Cancer
Authors: Zeng Tianyu , Xu Hai , Liu Yincheng , Yang Yiqi , Yang Fan , Yang Mengzhu , Sun Chunxiao , Bao Shengnan , Hua Yijia , Yan Xueqi , He Yaozhou , Wang Xi , Jiang Mengping , Liang Yan , Huang Xiang , Li Jun , Fu Ziyi , Li Wei , Yin Yongmei
Published: August 2021

Background

With increased survival in breast cancer, resulting from advances in treatment, patients incur the possibility of subsequent primary malignancies, especially lung cancer. The aim of this study was to assess the frequency of CT-detected pulmonary ground-glass nodules and lung cancer following breast cancer diagnosis, the associations between breast cancer and lung cancer, the pathological features of double primary cancer, and the status of epidermal growth factor receptor (EGFR) mutations in second primary lung cancer.
Methods

Clinical data from more than 8000 individuals who were diagnosed with primary breast cancer at Jiangsu Province Hospital (Jiangsu, China) between January 2008 and December 2020 were retrospectively analyzed.
Results

Of the 8048 patients, 55 (0.7%) were diagnosed with a second primary lung cancer, which accounted for approximately 14.8% of the pulmonary ground-glass opacity (GGO) detected. The incidence was higher than in the general female population (standardized incidence ratio 1.4 [95% confidence interval (CI): 1.25–1.55]). Patients who experienced a second primary lung cancer exhibited a significantly higher rate of EGFR mutation (78.5%) than those with lung adenocarcinoma alone, with most exhibiting low-grade malignancy, older age, estrogen receptor negativity, low Ki67, and no lymph node metastasis.
Conclusions

Breast cancer patients, especially those with low-grade malignancy, were at high risk for developing primary lung cancer. For isolated GGO in patients with high-risk factors, clinicians should insist on close follow-up. Furthermore, EGFR may play an important role in primary lung adenocarcinomas and breast cancer.
EZR Promotes Pancreatic Cancer Proliferation and Metastasis by Activating FAK/AKT Signaling Pathway
Authors: Xu Jian , Zhang Wei
Published: August 2021

Background: As a member of the ERM (ezrin-radixin-moesin) protein family, EZR has been recognized as a regulator of adhesion signal pathways by researchers. Moreover, EZR was thought to play irreplaceable roles in invasion and metastasis of versatile cancers. In this study, we managed to undermine the effect of EZR on proliferation and metastasis in pancreatic cancer (PC).Methods: To analyze the impact of EZR expression on overall survival and free diseases survival of PC patients, we screened abnormally expressed EZR in PC using the Gene Expression Omnibus database(GEO database) and The Cancer Genome Atlas (TCGA) database. Following, Gene Ontology (GO)-based functional analysis and Gene set enrichment analysis (GSEA) was performed to predicate the possible biological processes in which EZR were involved. The clinicopathological characteristics and prognosis of PC patients were analyzed according to clinical data. Further, immunohistochemistry, western blotting and real time PCR analysis were conducted to analyze the expression level of EZR in PC and paired paracancerous tissues. The effect of EZR on proliferation of PC cell lines were detected by Cell Counting Kit-8 assay, and meanwhile, Transwell assay was performed to detect the effect of EZR on invasion and migration of PC cell.Result: EZR exhibited higher expression level in pancreatic cancer tissues and cell than paracancerous tissues and cell, and its expression level was positively correlated with poor overall survival and diseases-free survival in PC patients. CCK8 assay indicated that EZR facilitated the proliferation of PC cells, meanwhile, Transwell assay showed that EZR promoted the migration and invasion of PC cells. The GO analysis predicated that EZR was involved in biological processes including cell adhesion, ameboidal-type cell migration, cell junction assembly. Through GSEA analysis, pancreatic cancer pathway, and the adhesion junction pathway were screened as the mostly enriched pathways in EZR-regulated pathological process. The inhibition of EZR suppressed proliferation and migration of PC cells. Western blot experiment revealed a positive correlation between EZR and FAK, the proliferation invasion and migration ability of PC cells were significantly decreased after knockdown of EZR.Conclusion: Our finding revealed EZR accelerated the progression of PC via FAK/AKT signaling pathway.
RPS27A is a Poor Prognostic Predictor for Hepatocellular Carcinoma via Interacting with METTL3-Mediated RNA Modifications
Authors: Liu Huiying , Zhu Jianjun , Kou Xiaoxia , Guo Lingling , Zhang Hongjuan , Chen Xiaofeng , Qiu Jinrong
Published: August 2021

Background: Increasing evidence has pointed to the involvement of RNA modifications in the pathogenesis of human cancers. However, they are rarely studied in hepatocellular carcinoma (HCC). Method: We summarized multiple types of RNA modification-related genes (RMRGs) from public references, and identified differentially expressed RMRGs (DEGs) between HCC tissues and matched normal samples, where their genetic variation were then investigated. The potential hub genes in the protein-protein interaction (PPI) network constructed by co-expression genes of RMRGs were recognized and verified in METTL3-knockdown HCC cell lines by quantitative PCR assay.Results: Seventy-six RMRGs, including six writers, seven readers, and seven erasers, were collected, of which 34 were identified and validated as DEGs. YTHDC2 exhibited the highest mutation rate, while ADAT2 showed widespread deletions. High correlations were observed between the expressions of 34 RMRGs. The PPI network constructed by 1080 co-expression DEGs related to RNA regulations consisted of 513 nodes and 11557 edges, with RPS27A presented the most directed edges and maximum closeness centrality. Patients with high expression of RPS27A showed worse overall survival (P < 0.01) and disease-free survival (P = 0.019). Moreover, RPS27A was found upregulated on high-risk metastatic and recurrent HCC tissues. Quantitative PCR assay indicated that RPS27A was significantly decreased in cancer cell lines when METTL3 was knocked down. Conclusions: Remarkable differences were observed for RNA modifications between HCC and normal samples, and RPS27A could be a poor prognostic predictor for HCC via interacting with METTL3-mediated RNA modifications.
Relationships between plant community productivity and diversity vary with trampling disturbance in an Inner-Mongolian steppe
Authors: Liu Yinzhan , Zhang Luna , Han Yangyang , Chen Anqun , Miao Renhui , Chen Ji , Wang Dong
Published: August 2021

The relationship between diversity and productivity of plant communities is an important issue in grassland restoration. However, the degree to which this relationship varies during the restoration stage after trampling disturbance is not clear. Here, we conducted a five-year study in a steppe after 4-year trampling to detect restoration patterns of plant community and investigate variation in diversity-productivity relationships. Our results showed that community cover, abundance, height, and productivity recovered quickly after the trampling disturbance ceased. However, the recovery of diversity was slower than biomass in the steppe. In addition, grass, annual, and biennial recovery was more rapidly than the recovery of forbs in the steppe. Moreover, following the restoration process, the positive correlation between productivity and diversity was decoupled, and a negative correlation between productivity and diversity developed. Our finding provides the key evidence for the asynchronous relation between productivity and diversity, and reveals that grass restored more rapidly than forbs in plant community restoration after disturbance. This study indicates that the trade-off between plant community structure and function can vary with the restoration process, and implicates that future modeling and experimental studies should focus on the different responses of productivity and diversity in plant community restoration.
Failure Analysis of A Welded Mild Steel Shaft Under Tensile Loading Condition
Authors: Hussain Arshad , Hussain Fida , Sattar Abdul , Hussain Tanweer , Bakhsh Qadir , Ahmed Muhammad
Published: August 2021

Welding is a process of permanently joining two or more parts by the application of heat and (or) pressure and is the widely used process in fabrication, maintenance and repairs of parts and structures. Almost 70 percent of all manufactured products are made with the skills of welders. In the field of Mechanical Engineering the modern welding trends and advanced products are made with the skills of welders. In the field of Mechanical Engineering the modern welding trends and advanced welding process has been contributing toward advancement, a study has been given greater priority of failures analysis of welding joints and various structural and technical aspects have an impact on fracture characteristics of welded joints. The welded joints and various structural aspects have an impact on fracture characteristics of welded joints. The objectives of this work were to investigate the tensile behavior of welded mild steel shaft to analyzing the failure analysis of welded joints for that purpose step joint, lap joint, double v joint, double v pin joint and double j joint specimens of standard size were developed. Each specimen was machined, welded and finished at its center. A tensile test was performed on all the welded shaft of mild steel. Tensile test has been carried out by Universal Testing Machine, after testing the specimens, it was found that the welded shaft with Lap Joints bears the Maximum Tensile Strength.
Multiobjective Site Selection Model for Emergency Shelter Facilities in Urban Areas
Authors: Yen Kuo-Chi , Chang Weid , Shih Wu-Chiao
Published: August 2021

Industrial and economic development is primarily applied to densely populated urban areas. If a sudden disaster occurs in such areas, the consequences can be severe. Shelter facility location affects the implementation of postdisaster relief work. This study explored residents’ perceived utility of evacuation time, their risk utility for road blocking, and the cost factors associated with constructing shelter facilities in the context of governance. A location model for emergency shelter facilities in cities was established on the basis of the aforementioned factors. Because the resolution of the random-weighted genetic algorithm (RWGA) is susceptible to influence from random weights, a robustness random-weighted method (RRWM) was developed. The validity and feasibility of the location model were examined through numerical analysis. Finally, the convergence of the RRWM was analyzed and compared with that of the RWGA and a single-objective genetic algorithm. The results revealed that the proposed algorithm exhibited satisfactory performance and can assist in evaluation and decision-making related to the selection of urban shelter facility locations.
History of Atopy Confers Improved Outcomes In IDH Mutant And Wildtype Low-Grade Glioma
Authors: Jaman Emade , Zhang Xiaoran , Sandlesh Poorva , Habib Ahmed , Allen Jordan , Saraiya Raj G. , Zinn Pascal O.
Published: August 2021

Purpose:A history of atopy or allergy has been shown to be protective against the development of glioma, however the effect of atopy on patient outcomes, especially in conjunction with the survival benefit associated with IDH mutation, has not yet been investigated, and is the focus of the study we present here. Methods:Low grade glioma (LGG) data from the TCGA was downloaded, along with IDH, TERT, 1p/19q and ATRX mutational status and genetic alterations. History of asthma, eczema, hay fever, animal, or food allergies, as documented in TCGA, was used to determine patient atopy status. Patients with missing variables were excluded from the study. Results:374 LGG studies were included. Patients with a history of atopy demonstrated longer overall survival (OS) compared to those without (145.3 vs. 81.5 months, p=00.0195). IDH mutant patients with atopy had longer OS compared those without atopy (158.8 vs. 85 months, p=0.035). Multivariate cox regression analysis demonstrated that the effects of atopy on survival were independent of IDH and histological grade, (p=0.002, HR 0.257, 95% 0.109-0.604), (p=<0.001, HR 0.217, 95% 0.107-0.444), and (p=0.004, HR 2.72, 95% 1.373-5.397), respectively. In terms of treatment outcomes, patients with atopy did not differ in treatment response compared to their counterpart. Pathway analysis demonstrated an upstream activation of the BDNF pathway (p=0.00027). Conclusion:A history of atopy confers a survival benefit in patients with diffuse low-grade glioma. Activation of the BDNF pathway may drive the observed differences.
Heavy Metal Contamination and Ecological Risk Assessment in Fluvial Sediment of San Juan –Taxco River System in Mining Region of Taxco Guerrero, Mexico
Authors: Sanchez Edith R. Salcedo , Morales Manuel Martínez , Mart Juan Manuel Esquivel , Mendoza Oscar Talavera
Published: August 2021

The hydrological system of San Juan-Taxco Rivers in located inside of one of the oldest and major mining district in Mexico. Several communities in the area use the rivers water along its flow for domestic water supply and crop irrigation. Sediment is an essential, integral and dynamic part of river basins, in polluted environments these act as sink of heavy metals and as a source of contaminants on the fluvial system. The management and sustainability of sediment should be assessed and secured to achieve good ecological status of the basins, for this task, approaches as ecological risk identification and geochemical indices are being used.Superficial sediment samples were collected in San Juan-Taxco river system. The results demonstrated that the degree of pollution from thirteen heavy metals and metalloids studied decreases in the following sequence: Cd> Zn > Pb > Cu > As > B > Mn > Ni > Fe > Co > Ba > Al and Cr. Cd made the most dominant contribution. Geochemical indices revealed important external anthropogenic influences in the rivers. The geochemical indices indicated very high enrichment for As, Cu, Pb and Zn, and extremely high for Cd in the three-rivers. The calculation of Pollution Load Index (PLI) showed in Cacalotenago River and in Taxco River are the sites with the highly contaminated sediments. PLI values were very high in all the samples sites due mining tailings erosion, wastewater and agriculture run off. Cd, Zn, Pb, Cu and As were the main potential risk elements that will cause harmful biological effects in the riverine environment.
hAMSC-CM Attenuates Paraquat-Induced A549 Cell Damage by Reducing Endoplasmic Reticulum Stress and Oxidative Stress
Authors: Liao Futuan , Gong Liming , Jia Lijing , Wang Jianhong , Liu Tongying , Huang Tingting , Liang Jing , zhou manhong
Published: August 2021

Acute paraquat (PQ) poisoning results in severe acute lung injury and pulmonary fibrosis, and there is no specific antidote; thus, the mortality rate of PQ poisoning is extremely high. The mechanism of poisoning may be associated with endoplasmic reticulum stress, oxidative stress damage and organ/tissue inflammation. Recent studies have reported that human amnion-derived mesenchymal stem cells (hAMSCs) secrete a variety of cytokines, and that hAMSC-conditioned medium (CM) has anti-inflammatory and immunomodulatory effects. The aim of the present study was to investigate whether hAMSC-CM exerts protective effects against PQ toxicity in A549 cells. The data demonstrated that the activity of A549 cells was decreased after 24 h of PQ exposure and that the cell viability of the hAMSC-CM intervention group was higher compared with the PQ-only group. hAMSC-CM intervention decreased cell damage, apoptosis rates, oxidative stress indexes, Bax/Bcl-2 ratios and CHOP expression levels in poisoned cells by CCK-8 experiment, apoptosis detection, ROS content detection, and Western blot analysis respectively. In conclusion, hAMSC-CM may attenuate the cell damage caused by PQ by reducing endoplasmic reticulum stress and oxidative stress.