bluuebunny/crossref_metadata_2025 huggingface dataset:
New records of Boana icamiaba (Anura: Hylidae) in the Brazilian Amazon rainforest
Authors: Dos Anjos Samuel F. , Wronski Wanne S. S. , Penhacek Marcos , Noronha Janaina Da Costa , Pinto Karll C. , Oda Fabrício H. , Rodrigues Domingos J.
Published: July 2019

Boana icamiaba is an Amazonian anuran species reported for sites in the mid-lower Madeira-Rio Tapajós River and lower Tapajós-Rio, and Xingu River interfluves – municipalities of Juruti, Altamira, Santarém, and Itaituba, state of Pará, northern Brazil. We provided the first records of Boana icamiaba for the states of Mato Grosso and Rondônia, central and northern Brazil, which enlarges the knowledge on its distribution in approximately 470 km southmost and circa 886 km southwestmost from the nearest previously recorded locality.
A linguagem em Descartes
Authors: Haubert Laura Elizia
Published: July 2019

O presente artigo tem como objetivo apresentar a concepção cartesiana de linguagem a partir de excertos recolhidos de cartas e da obra maior de René Descartes. A exposição foi dividida em três partes: (i) primeiramente, faz-se uma breve apresentação geral sobre o tema da linguagem na filosofia cartesiana; (ii) em seguida, faz-se uma análise de excertos do autor divididos em quatro subtópicos: (a) atendendo ao caráter corpóreo e incorpóreo da linguagem; (b) à natureza dos animais; (c) ao argumento da linguagem; e (d) à querela da gramática universal; e (iii) conclusão que reúne os pontos principais abordados.
As possibilidades do conhecimento na seção consciência e o papel da linguagem na filosofia de Hegel
Authors: Da Silva Oliveira Luiz Filipe
Published: July 2019

Este artigo tem por objetivo analisar, no interior da seção Consciência da Fenomenologia do Espírito de Hegel, as possibilidades de conhecimento analogamente às especificidades de expressões linguísticas, elencando assim as particularidades de cada uma das figuras da consciência representadas nas subseções sobre a Certeza sensível, a Percepção, e Força e entendimento. Não obstante, nossa análise não visa apenas adentrar nos pormenores de tais figuras, mas contextualiza-las no interior de uma disposição conceitual presente no interior do pensamento hegeliano. Mais especificamente, no interior de uma possível teoria da linguagem que perpassa não somente a Fenomenologia, mas se dispõe também no trato que Hegel apresenta em sua Lógica e Estética.
Knowledge Creation from the Perspective of the Supply Chain. The Role of ICT
Authors: Kő Andrea , Vas Réka , Kovács Tibor , Szabó Ildikó
Published: July 2019

Technological innovations are inclining the world of business to restructure actual business processes at the threshold of the fourth industrial revolution. These circumstances create knowledge-intensive organizational, collective and personal learning environments in which ICT tools play a critical role. This paper investigates knowledge creation patterns inherent in the supply chain of companies that operate in a networked environment in the Székesfehérvár region of Hungary. ICT solutions applied in knowledge creation and collaboration with suppliers and customers in the supply chain were studied in this research. One of the main contributions of the paper is the study of knowledge creation patterns in three dimensions: the Socialization – Externalization – Combination – Internalization (SECI) framework, supply chain processes and ICT solutions, which is a unique approach compared with the frameworks from the relevant literature.
OPTIMISATION OF ORGANIC SOLVENT EXTRACTION OF TOTAL CAROTENOIDS FROM BULGARIAN TOMATO PROCESSING WASTE
Authors: Nikolova M. , Prokopov T. , Taneva D. , Dimitrov N.
Published: July 2019

The quantity of tomato wastes combined with beneficial characteristics of components of the wastes justifies the great interest of researchers and manufacturers in extracting of carotenoids from this low cost material. In this study the response surface (RSM) approach and Box-Behnken design (BBD) were used to explore the possibility of modelling and optimisation the organic solvent extraction of total carotenoids from Bulgarian tomato processing waste (TPW).  Bulgarian TPW consists of tomato pomace was used in this study. Dried and ground TPW was plased in the extraction flask and stirred with acetone at various extraction conditions. The extract obtained was vacuum filtered through filter paper and was subjected to spectrophotometrically total carotenoids content determination. A three-variable, three-level BBD of RSM was employed in optimisation the extraction conditions for the highest recovery of total carotenoids from Bulgarian TPW. A response surface quadratic model was developed and statistical analysis was carried out. Data concerning model adequacy tests indicated that the values of R2 and Radj2 for quadratic model were the higest in comparison to the other models. The obtained F-value of 38.65 implies the model was significant (p < 0.05) and could be used for optimisation. The effects of extraction temperature, solvent to solid ratio and extraction time were significant in total carotenoids yield. Total carotenoids content ranged from 9.78 to 25.28 mg/100 g dried TPW. The predicted values of total carotenoids content were closed to the experimental observed values. By use of RSM the optimal extraction conditions were determined as follow: extraction time of 90 min, solvent to solid ratio of 60 mL/g and extraction temperature of 50ºC. The results obtained showed that predicted (28.40 mg/100 g dried TPW) and experimental (28.86±0.92 mg/100 g dried TPW) values of total carotenoids content were not significant different (p > 0.05).
ADJUSTING FLOUR QUALITY BY ENZYMES: CURRENT STATE, PROBLEM ANALYSIS, FUTURE DEVELOPMENT PROSPECTS
Authors: Zhygunov D. , Marchenkov D. , Lebedenko T.
Published: July 2019

The article overviews the issue of wheat flour modification by enzymes. The role of enzymes in the dough formation process is considered. Modern ways of providing the desired dough parameters for flour products in conditions of Ukraine are shown. Recommendations and suggested directions for further research are given. Flour is a complex multicomponent product and have to correspond with a number of requirements for its composition and properties. Different conditions of grain cultivation and storage result in significant deviations of its quality indicators when it comes to flour mills. The modification of flour going through adding several technological additives, in particular by enzyme products. The action of enzymes to a large extent allows to adjust the properties of the dough and of flour end-products. In addition, enzymes further affect the nutritional values of flour, which makes it possible for the flour production to use low-quality grain, while maintaining the planned quality indicators of flour. The functional properties of flour fractions obtained on different technological passages depend on the content of various anatomical parts of the grain from which they derived from. Particle size, starch damage, protein content, fat content, ash content and intensity of enzyme activity vary significantly depending on the type of grinding equipment. All this gives reason for recommending the introduction of enzymes not while manufacturing bakery end-products but still at the stage of flour production. The damage to the grain with a corn bug, grain germination in Ukraine puts grain-processing plants the task of assessing the activity of own grain enzyme systems. Indirectly, this can be estimated using the gluten deformation index and the grain Falling Number. But the estimation of enzyme systems by such methods does not allow precisely to calculate the amount and composition of enzyme products necessary to achieve maximum effect when adjusting flour properties. The issue of removing anti-nutrient factors in flour, which is largely inhibitors of the action of both their own grain enzyme systems and additionally introduced enzyme preparations, is also relevant.
FUNCTIONAL FOODS: PROSPECTS IN UKRAINE
Authors: Kaprelyants L. , Yegorova A. , Trufkati L. , Pozhitkova L.
Published: July 2019

Today, food is intended not only to satisfy hunger and provide necessary nutrients for human organism, but also to prevent nutrition-associated diseases and improve the mental and physical condition. The formula of food in the XXI century includes constant use of both traditional natural foods and foods with desired properties in the diet. The foods with desired properties are the functional food products, i. e. those enriched with essential food substances and micronutrients. These functional products cover a wide range of foods and ingredients with various biologically active substances responsible for their health-supporting and preventive effectiveness. This allows to lower the healthcare expenditures and support the economical development. The health and general well-being of today’s Ukrainians is influenced by many factors: rush life tempo, age-related changes in organs and tissues, heredity, unhealthy habits, stress situations etc. But the great importance of the diet and numbers and quality of products from which meals are prepared also should be kept in mind. And while we cannot change some factors, we can absolutely control others, particularly the characteristics of foods. The article presents some features of modern diet of Ukrainian population as an integral factor of health risk. Also, we show food substance consumption profiles of Ukrainian population in years 2015-2018, and the rate of functional foods on Ukrainian market in the last two years. The article is a review and highlights the necessity to develop production of functional foods based on fundamental and applied researches in food technology, nutritiology and the latest molecular biological technologies. General approaches to modern principles of functional food production technology are proposed. The role of the latest molecular biological technologies in functional food production is disclosed, especially of the proteomics, nutrigenomics and metabolomics. Important directions of functional food research are shown, they are associated with identification of new functional ingredients and increasing confidence of the consumers in such products. Motivations are proposed to stimulate the food manufactures to continue to develop the functional foods and to increase their assortment, thus providing a remarkable profit from successful products.
USE OF OILSEED POLYFUNCTIONAL SUPPLEMENTS IN THE MANUFACTURE OF MEAT PRODUCTS
Authors: Topchii O. , Kotliar Ye. , Honcharenko T. , Petryna A. , Tarasiuk O.
Published: July 2019

Nowadays, meat production is developing towards a wider range of products. Their components are optimised and combined in such a way as to increase the nutritional value, to preserve the most valuable components of raw materials, to make for some lacking macro- and micronutrients by including the functional ingredients in the recipe. A possible way to improve the meat products technology is making purposeful changes in the formulation of the product. This involves selecting certain types of raw materials and additional components in ratios providing the required nutritional value of the finished product. New functional ingredients having the desired physiological effects on the human body should be combined with new, modern means of processing raw materials that will provide the necessary technological characteristics, the quality and safety of the final product. Home-manufactured vegetable supplements obtained from seeds of different crops are a promising direction of research. Their unique chemical composition and functional properties allow achieving the expected nutritional value of the finished product in accordance with modern nutritional standards. This article presents the results of analysing and studying of oilseeds aimed at creating a balanced multifunctional supplement to be used in recipes of functional meat products. It has been studied how the functional and technological parameters of seeds change depending on the degree of their grinding. The organoleptic evaluation has allowed determining the optimum ratio of plant components in the mixture. The quality parameters of the supplement developed, its biological value, vitamin and mineral composition have been determined. The organoleptic characteristics and structural and mechanical parameters of meat model systems have been analysed, which has made it possible to recommend the right percentage of the composite oilseed mixture in the recipes of meat products, namely, pates and chopped semi-finished products.
THE PROBLEMS OF MEAT PRODUCTS THERMAL TREATMENT
Authors: Vinnikova L. , Synytsia O. , Kyshenia A.
Published: July 2019

The main problems of meat products thermal treatment have been considered and analysed in the paper. Analysis of the existing research papers suggests that the main attention is devoted to the influence of temperature value and time of processing on the physical, chemical, microbiological and organoleptic indicators of the product. The influence on the protein and fat components of raw meat has been described as well as the changes in the meat product structure under the influence of temperature have been pointed out. The important impact of thermal treatment on the changes in proteins at heating, including solubility and hydration of the proteins, has been examined. The paper analyses the microbiota screening before and after thermal treatment. Special attention was paid to the impact of temperature on microbiota as it is an important indicator of a meat product safety. Methods and purpose of thermal treatments as well as the advantages and drawbacks of each method were considered. Formation of tastiness properties of the product depending on the methods and thermal cycling was emphasized. Submitted is the analysis of the factors that influence the product mass losses under the impact of high temperature. The dependence conditions of a meat product quality on the thermal cycle were also analysed. Attention is paid to alternative methods of thermal heating as well as their influence on ready product quality and safety. Substantial contribution of Odesa National Academy of Food Technologies to solving problems of the conventional thermal treatment of meat products was shown, same as a possibility of bringing a product to cooking readiness without application of high temperature. The main content of the paper includes analysis of the methods and thermal treatment cycles existing in the world and proves importance of the temperature control and cooking time which influence safety, quality and yield of the product.
RESEARCH OF THE SAFETY INDICATORS OF BERRY SAUCES WITH SEAWEED’S RAW MATERIALS
Authors: Deinychenko G. , Lystopad Т. , Kolisnychenko T.
Published: July 2019

The current thing now is the matter regarding enrichment of food products with organic iodine, which is caused by iodine deficiency in the food ration of most of the country's population and by the country-wide radionuclide pollution, which leads to a hundredfold increase of risks of the thyroid body radiation. In this regard, there were developed the technologies of the berry sauces with the addition of the seaweed’s raw materials, as the iodine-concentrating component. The technologies of blueberry-cranberry sauces with guelder-rose juice, cornel-bilberry sauce with guelder-rose juice and blueberry-buckthorn sauce with guelder-rose juice were developed. This work is dedicated to a research of safety indicators of the developed sauces. There were used the hydrated seaweeds of Laminaria, of Fucus, of Undaria pinnatifida as iodinated additives. The following microbiological indicators were determined: the quality of mesophilic aerobic and optionally anaerobic microorganisms (МAOАnM), mold, yeast and lactic acid bacteria, and the presence of the coliform bacteria (CB) bacteria. The study results showed, that there are no microorganisms in the finished products, which may result the damage of the product after pasteurization. It was identified, that the own organic acids of the prepared sauces will be sufficient to ensure the microbiological clearness and the use of the additional preserving agents does not make sense. Toxicological indicators, namely the mass fractions of lead, cadmium, arsenic and mercury, have been studied. It has been established that the content of toxic elements in test samples is hundreds of times less than permissible. The toxicological indicators were studied, namely, the mass fractions of lead, cadmium, arsenic and mercury. It was identified, that the content of toxic elements in test samples is hundreds of times less than permissible. Thus, it is proved that the sauces made according to the developed technology, comply with the requirements of the regulatory documents according to the safety indicators. Moreover, it is proved, that that the addition of the seaweed’s raw materials does not worsen these indicators. To confirm the obtained data, the sample with the highest seaweed content was examined by an independent laboratory accredited according to DSTU ISO/IEC 17025 with the issuance of the appropriate test report.
Biochemical Characterisation and Sensory Evaluation of Differently Coloured and Shaped Tomato Cultivars
Authors: OLUK Aylin Celile , ATA Atilla , ÜNLÜ Mustafa , YAZICI Ebru , KARAŞAHİN Zafer , EROĞLU Evren Çağlar , CANAN İhsan
Published: July 2019

Antioxidant capacity, total phenolic content, colour, sugar, volatiles, ascorbic acid and carotenoid (β-carotene and lycopene) contents of differently coloured and shaped tomato cultivars (cvs) grown in the Eastern Mediterranean region, Turkey were determined, along with a sensory evaluation. Tomato cultivars of two different types (cherry and beefsteak) and four different colours (red, yellow, orange and brown) were analysed. All plants were simultaneously grown in the same field and subjected to identical horticultural practices to minimise the effects of environmental conditions and to maximise those related to genotype. The red cherry cultivar had the highest lycopene content, while the orange beefsteak cultivar had the highest β-carotene content. The highest antioxidant capacity, total phenolic content and hardness scores were found in cherry-type tomatoes, except the yellow one. The red cherry cultivar had the highest sugar content. Red and brown cherry cultivars were also significant in terms of their high carotenoid and sugar contents, along with a high antioxidant activity. The brown cherry cultivar had the highest total phenol content. The highest quantities of 2-hexenal, 3-hexen-1-ol, and 6-methyl-5-hepten-2-one were detected in red cultivars. The brown cherry cultivar had the highest sweetness, typical aroma and hardness scores, while the yellow beefsteak cultivars the lowest sweetness typical aroma scores. In terms of sensory parameters, red and brown cultivars scored higher than yellow and orange ones.
 
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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Nutritional Value of Garden Dill (Anethum graveolens L.), Depending on Genotype
Authors: BIESIADA Anita , KĘDRA Kamil , GODLEWSKA Katarzyna , SZUMNY Antoni , NAWIRSKA-OLSZAŃSKA Agnieszka
Published: July 2019

The yield, chemical composition and antioxidant activity of eight genotypes (‘Krezus’, ‘Turkus’, ‘Kozak’, ‘Szmaragd’, ‘Lukullus’, ‘Herkules’, ‘Ambrozja’, ‘Moravan’) of garden dill (Anethum graveolens L.) biomass was estimated. Field experiments were conducted in 2011-2013 in Poland. The crop lasted 44 days and plants were collected at the 4-5 true leaf stage (a bunch harvest). The highest marketable yield was obtained for ‘Krezus’ and ‘Szmaragd’ cultivars (1.18 kg·m-2), while the lowest for ‘Herkules’ (0.53 kg·m-2). The dry matter of leaves ranged from 15.17% (‘Ambrozja’) to 19.27% (‘Krezus’). The study also proved that the genotype influenced the content of chlorophylls a+b, e.g. 1.10 g·kg-1 (‘Moravan’) and 0.78 g·kg-1 (‘Lukullus’), respectively carotenoids: 21.43 mg·100 g-1 (‘Moravan’) and 11.78 mg·100 g-1 (‘Szmaragd’). The content of nitrates (371.48 mg·kg-1 ‘Lukullus’ - 110 mg·kg-1 ‘Szmaragd’), K (11.30% ‘Moravan’ - 5.37% ‘Kozak’), Ca (2.06% ‘Turkus’ - 1.77% ‘Kozak’), and oils (99.13% ‘Lukullus’ - 93.82% ‘Ambrozja’) was also varied. In most cases the content of Mg, P, sugars and ascorbic acid was on similar level. The antioxidant activity was significantly different between tested groups (12.22 µM·g-1 ‘Turkus’ - 6.27 µM·g-1 ‘Krezus’). The presented research proved that the genotype of garden dill affects yield and chemical composition of plants.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Influence of Selected Antibiotics on the Tomato Regeneration in In Vitro Cultures
Authors: GERSZBERG Aneta , GRZEGORCZYK-KAROLAK Izabela
Published: July 2019

Generally, antimicrobial agents are frequently used in micropropagation techniques to obtain free elite clones or after genetic transformation to select putative transformants. Their successful application minimizes bacterial contamination however; they may be phytotoxic and may diversely affect the regeneration ability in plant tissue cultures. The objective of the current study was to estimate the effects of four antibiotics i.e. ampicillin, carbenicillin, cefotaxime and kanamycin on morphogenesis of three Polish tomato cultivars, cultured on MS medium with phytohormones. In this experiment the ability of tomato cotyledon explants to regenerate entire plants via indirect organogenesis was tested. Among four antibiotics tested, kanamycin was most harmful for the explants. This antibiotic, even at low doses (10-20 mg/L), inhibited tomato morphogenesis. On the other hand, the current study revealed significant influence of different concentrations of ampicillin, carbenicillin, cefotaxime on the frequency of bud formation. While the addition of cefotaxime at low concentration (100-200 mg/L) stimulated the bud formation, its increasing concentration adversely affected the organogenesis of tomato. The results clearly pointed out that carbenicillin and ampicillin at low concentrations (100-400 mg/L) were not only non-toxic, but they promoted bud regeneration. The obtained results show the crucial role not only of use of efficient antibiotics, but also of their proper doses in obtaining successful transformation and regeneration of tomato.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Biocontrol of Pear Postharvest Decay by Kombucha
Authors: TAN Junping , ZHOU Xian , CHENG Shuiyuan , CHEN Zexiong , GOU Yuanyuan , YE Jiabao , XU Feng
Published: July 2019

Kombucha is sweetened, slightly alcoholic, and lightly effervescent tea drink. Its acidity inhibits the growth of harmful bacteria. Here, we studied effect of kombucha on post-harvest preservation of pear. The Hosui pears were soaked with the kombucha for 15 minutes, and in distilled water as the control, respectively. Superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), hydrogen peroxide (H2O2), proline content, electric conductivity, weight loss rate and good fruit rate in pear were measured during storage at room temperature. The results showed that the kombucha could effectively prolong the storage time of pear fruit. The fruit quality of the treated group was 1.5 times higher than that of the control pear fruit when stored at room temperature for 18 days. The weight loss rate, MDA content and electric conductivity of the treatment group were lower than those of the control group during the whole storage period, indicating that kombucha can inhibit the transformation of polysaccharides such as starch and pectin, and delay the degradation of nutrients in the fruit, resulting in a decrease in weight loss rate, inhibition of membrane lipid peroxidation, reducing MDA content, electric conductivity, maintaining cell membrane stability, delaying pear fruit senescence. Kombucha can inhibit the content of H2O2 and proline, increase the activity of POD and SOD increase the storage stability of fruits. The purpose of this study was to elucidate the physiological mechanism of post-harvest preservation of pear by kombucha. The use of kombucha can prolong the supply period of pear, increase economic benefits and expand the market of pear.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Optimization of the Solid-Liquid Extraction Process of Phenolic Compounds from Mulberry Fruit
Authors: KOSTIĆ Emilija , ARSIĆ Biljana , MITIĆ Milan , DIMITRIJEVIĆ Danica , PECEV MARINKOVIC Emilija
Published: July 2019

The study was designed to examine the influence of solvent concentration (ethanol/water 20-80% by volume), extraction time (15-240 min) and extraction technique on the extraction yield of phenolic compounds, flavonoids and antioxidant activity from Morus nigra L., Morus rubra L. and Morus alba L. fruits. The optimum conditions for the extraction of total phenols, flavonoids and monomeric anthocyanins by the maceration and ultrasonic extraction processes were: 213.6 min with 80% ethanol, and 182.1 min and 71.2% ethanol, respectively (black mulberry), and 216.5 min with 78.3% ethanol and 198.7 min and 70.6% ethanol, respectively (red mulberry). In case of white mulberry for the extraction of total phenols and flavonoids optimum extraction conditions (maceration and ultrasonic) were 232.7 min and 80% ethanol, and 187.2 min and 68.7% ethanol, respectively (white mulberry). Obviously, ultrasonic extraction was less time consuming, and it requires for all performed extractions solvent with less percentage of ethanol.
 
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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba
Authors: LI Linling , YUAN Honghui , ZHA Sanxing , YU Jie , XIAO Xian , DENG Kun , CHENG Shuiyuan , CHENG Hua
Published: July 2019

Ginkgo biloba is a unique tree in China with medicinally and phylogenetically important characteristics. Terpene trilactones (TTL) is a key active pharmaceutical ingredient in Ginkgo, so the content of TTL in Ginkgo has become one of the important indices for evaluating quality of the medicinal materials. By transcriptome sequencing on samples treated by chlormequat, ultraviolet (UV) and drought, totally 59820 contigs and 37564 unigenes were obtained. Furthermore, 18234 unigenes were annotated through COG, KEGG and GO analysis. There were 78 AP2/ERF transcription factors, 23 factors of up-regulation and 66 factors of down-regulation that were related with synthetic pathway of TTL in Ginkgo. Phylogenetic tree clustering analysis indicated that there were 42 AP2s could be clustered into ERF, DREB and RVA subfamilies. EMSA analysis demonstrated that GbERF13, GbERF25 and GbERF27 could bind with regulatory elements, such as E-box, in the upstream of GbMECPs promoter. Expression analysis showed that the expression level of GbERF25 was the highest in root, and GbERF25 and GbERF27 were expressed in relatively high transcription levels in leaf and other tissues. The results of qRT-PCR indicated that CCC treatment could significantly improve expression levels of ERF25 and ERF27, and UV and drought could induce transcription levels of ERF13 and ERF25, respectively. The results implied that ERF25 and ERF27 might involve in the induction and regulation of CCC treatment on synthesis of bilobalide in G. biloba. ERF13 might participate in the regulation of bilobalide synthesis induced by UV, and EFR25 might involve in the regulation of the synthesis induced by drought. During annual cycle of expression, the transcription levels of ERF13, ERF25 and ERF27 had significantly positive correlation with diterpene level with correlation coefficient 0.975. It implied that these transcription factors mainly acted on the MEP pathway that regulated synthesis of bilobalide. The aim of the research was to indicate the mechanism of environment or cultivation measure regulating target gene of TTL metabolic pathway by AP2/ERF, and establish metabolic network of AP2/ERF regulating TTL synthesis.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Cytological Investigation in Two Mutants of the Hybrid Grapevine ‘Niagara’
Authors: PIEROZZI Neiva Izabel , MOURA Mara Fernandes
Published: July 2019

‘Niagara’ is a hybrid table grape (V. vinifera × V. labrusca). Several spontaneous somatic mutants from this grapevine have already been described. However, cytological information about these mutants is scarce. Therefore, studies in the microsporogenesis have been carried out in two of these mutants, a seedless and a large berry mutant (giant mutant). A low percentage of abnormalities such as precocious chromosome segregation, bivalent not aligned, laggard and stickiness chromosomes were seen in both mutants.  A merging process of microspores was observed in several tetrads in the seedless mutant: the microspore came together by large cytoplasmic bridges giving rise to a large and amorphous single-celled tetrad, which eventually collapsed. The giant mutant showed 10.29% of tetrads with microcytes. However, these tetrad abnormalities did not hinder pollen viability. Significant differences were seen concerning stomata and pollen grain sizes, stomata frequency and anther number per flower between the mutants. The chromosome count at diakinesis (n=19) and in diploid tapetal cells (2n=38) clarified the fact that the seedless somatic mutation was not associated with any ploidy event. Conversely, the giant mutant showed n=38 at diakinesis and 2n=76 in diploid tapetal cells, pointing to a case of spontaneous chromosome duplication. Since these abnormalities did not disturb the percentage of pollen viability very much, in both mutants (higher than 86%), the seedless one may be used as a male parent in crossing, regarding the desirable seedlessness character, while the mutant giant may be employed as female parent for the obtainment of seedless triploid plants.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Some Biochemical Characteristics of Fruit Samples of Sambucus nigra Genotypes from Northeastern Turkey
Authors: KAN Tuncay
Published: July 2019

There are numerous wild edible fruit species in the world and they are important components of dietary utilization, local pharmacopoeia, cultural and religious practices of indigenous people. In this study, some important berry characteristics of seven seed propagated black elderberry (Sambucus nigra) genotypes sampled from Rize province, Turkey, were assessed. The genotypes exhibited a great diversity on most of the biochemical characteristics. Berries of genotypes showed moderate Vitamin C content ranged from 26 to 38 mg per 100 g fresh weight. Total phenolic and total anthocyanin content in fruits of the S. nigra genotypes has also high value ranged from 360 to 447 mg gallic acid equivalent (GAE) per 100 g fresh weight and 307 to 412 mg cyanidin-3-glucoside equivalent per 100 g fresh berry, respectively. Antioxidant activity was found between 5.28 and 7.11 mmol trolox equivalent per 100 g fresh weight basis. Citric acid and fructose determined as major organic acid and sugar for all genotypes. For its high vitamin C, total anthocyanin and total phenolic content and high antioxidant activity of fruits of wild grown S. nigra may have provide many health benefits and can be domesticated to establish orchards in Turkey.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Characterization and Tissue-specific Expression of bHLH Genes in Dimocarpus longan
Authors: ZHENG Wei , DONG Xueming , YU Xuefei , ZHANG Qiuying , CHEN Ning
Published: July 2019

In plants, the basic helix-loop-helix (bHLH) transcription factors (TFs) play pivotal roles in many biological processes including growth, stress response, and secondary metabolite synthesis. To date, many bHLH genes have been identified and characterized in diverse plant species. However, little is known regarding the bHLH genes in Dimocarpus longan Lour. (D. longan). Based on RNA-seq data, we identified 42 putative bHLH genes from D. longan and determined their putative functions using the NCBI Conserved Domain Search Tool and Pfam databases. The physicochemical properties, phylogenetic relationships, conserved motifs, gene ontology (GO) annotations, protein-protein interactions, and tissue-specific expression patterns of these bHLH genes were systematically explored. In total, ten motifs were found in DlbHLH proteins using MEME, among which two were highly conserved. Phylogenetic tree analysis found that DlbHLH proteins can be divided into nine groups, with group 2 being the largest. GO annotation results showed that the DlHLH genes were involved in various molecular functions. RNA-seq and qRT-PCR results revealed important differences in the expression patterns of 17 of the DlbHLH genes. In particular, DlbHLH-9, DlbHLH-19, DlbHLH-25, DlbHLH-26, and DlbHLH-35 were found to show significantly different expression patterns in root and leaf tissues. The results of this study will further enrich our knowledge regarding bHLH transcription factor genes and lay a foundation for enhancing the production of active secondary metabolites by genetic engineering in D. longan.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Composition of Lavandula angustifolia L. cultivated in Transylvania, Romania
Authors: OROIAN Camelia , ODAGIU Antonia , RACZ Csaba Pal , OROIAN Ioan , MUREȘAN Iulia C. , DUDA Marcel , ILEA Marioara , BRAȘOVEAN Ioan , IEDERAN Cristian , MARCHIȘ Zamfir
Published: July 2019

An increasing market demand concerning lavender essential oil is reported. Identification and characterization of the main compounds of this oil is needed, in order to identify chemotypes. This information is useful for further uses of the produced oil. Essential oil extracted from two Lavandula angustifolia L. varieties, cultivated in different areas of Transylvania, Romania, was analysed from the point of view of qualitative and quantitative composition, using gas-chromatography. Basic statistics was used for calculation of essential oil compounds means and dispersion parameters, while the profile of the essential oil composition was emphasized using Box-Plot diagrams and cluster analysis. Nine compounds, in different amounts were quantified, and two chemotypes, corresponding to ‘Mailette’ and ‘Vera’ varieties were established (linalool, and linalyl acetate, respectively). Differences in essential oil composition are recorded in ‘Vera’ variety corresponding to ‘Vera’ linalyl acetate chemotype, function of specific condition of lavender cultivation area.
Various Light Quality including QD-LED Affect Growth and Leaf Color of Red Romaine Baby Leaf Lettuce
Authors: HAN Su Jung , CHOI In-Lee , KIM Ju Young , WANG Lixia , LEE Joo H. , CHOI Ki-Young , KIM Yongduk , ISLAM Mohammad Z. , LEE Young-Tack , KANG Ho-Min
Published: July 2019

Light quality has a substantial effect on crops in plant factories. Quantum dot (QD) refers to ultrafine semiconductor particles and is expressed in a variety of wavelength ranges and fine and precise colors and is attracting attention as a next-generation material. This study was conducted to investigate the effects of various light qualities, including a QD-LED light source on the growth and color development of red romaine lettuce in the LED chamber system for plant factories. The light source was red fluorescent (FL), blue (B), red (R), blue + red mixed light (BR), and blue + wide range red + red mixed light QD-LED. The growth index, chlorophyll content, and leaf color were examined. The results showed that the plant length was long without a significant difference in QD-LED and the red (R) wavelength and showed the fastest growth under QD-LED. The photosynthetic rate and chlorophyll content were the highest in QD-LED light with a broad spectrum of red light. The leaf colors a*, b*, the hue angle, and total anthocyanin content showed the highest in QD-LED light, while b* and the hue angle values ​​were the lowest. As the light wavelength range was changed, there was a significant difference in the growth response and leaf color, and it is considered that QD-LED light should be considered positively when applied to plant factories.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Wood Anatomy in Several Genera of Nigerian Annonaceae
Authors: OLATUNJI Olatunji Olusanya
Published: July 2019

The anatomical characteristic of the wood of Annona muricata, A. senegalensis, Xylopia aethiopica, A. glauca, A. squamosa, Cleistopholis patens, Monodora tenuifolia and Greenwayodendron suaviolis were investigated in search of their stable taxonomic attribute. Thirty-two wood samples were collected from eight species of Annonnaceae (four specimens each). Fixation of the most healthy and fresh wood of each species was done using 500 ml of FAA (Formalin Acetic Acid) and dehydrated in a series of ethanol while infiltration was done using tertiary-butyl-alcohol prepared in accordance with Johansen’s method. The sectioning was carried out with a rotary microtome and the slide containing the wood samples were examined using power shots s70 camera attached to computer. The results revealed several interesting wood anatomical features such as the presence of numerous fibre, ray cells, vessels, absence of axial parenchyma in some species, growth ring ranges from distinct to indistinct. Rays are composed of upright cells in A. muricata, fibre tracheid are also uncommon in A. muricata but common in G. suaviolis. Axial parenchyma are common in A. glauca and A. squamosa but absent in other species. The wood structure of A. glauca and A. squamosa are similar to that of C. patens, but the absence of axial parenchyma distinguished it from them. The results are important in understanding the relationships between and within the species.
Phenotypic aberrations during micropropagation of Soymida febrifuga (Roxb.) Adr. Juss
Authors: CHIRUVELLA Kishore Kumar , MOHAMMED Arifullah , GHANTA Rama Gopal
Published: July 2019

Like most of the medicinal plants Soymida febrifuga (Meliaceae) possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protocols for in vitro mass propagation has been established from field grown and aseptic seedlings explants. Strikingly, we observed aberrant structures such as vitrified shoots, faciated shoots, albino shoots as well shoot necrosis during its micropropagation. These phenotypic maladies were observed during organogenesis and rooting. Compared to other abnormalities, shoot necrosis nonetheless was frequent and pronounced leading to plant death. Shoots when subjected to rooting also displayed necrosis which was controlled by transferring to MS medium containing various concentrations and combinations of calcium levels, activated charcoal, glucose, fructose and auxins. Microshoots initiated roots on half strength MS medium with IBA and IAA individually or in combination within two weeks. MS half strength solid medium supplemented with CAN (556 mg l–1), CAP (1.0 mg l–1), IAA (2.0 mg l–1) and IBA (2.0 mg l–1) in combination was found to be more efficient in showing high frequency (95%) of root regeneration. Rooted plantlets were successfully hardened and 70-85% of regenerated plants were successfully acclimatized to natural environment. In vitro derived plantlets were morphologically similar to in vivo plants.
Activity of the Recommended and Optimized Rates of Pyridate on Chickpea - Mesorhizobium mediterraneum Symbiosis
Authors: PARSA Mehdi , ALIVERDI Akbar , HAMMAMI Hossein
Published: July 2019

Crop-rhizobium symbiosis can be influenced by leaching of herbicides which is unavoidable after their application. Due to an adjuvant which might help to develop the low-use-rate of herbicide, an experiment was carried out to compare the impact of the recommended rate (1200 g active ingredient ha-1) and the optimized rate (282.15 g active ingredient ha-1) of pyridate on the biological properties of eight chickpea cultivars inoculated with Mesorhizobium mediterraneum, grown in pots. Based on the required rate of herbicide to give 95% control of common lambsquarters (Chenopodium album L.) value, the efficacy of pyridate improved up to 3.87-fold by adding methylated rapeseed oil to spray solution. The ‘Desi’ cultivar had significantly higher nodulation than ‘Kabuli’ cultivar. In general, toxicity of the recommended rate was higher than the optimized rate. With the exception of root dry weight, all of the measured parameters were significantly affected by the recommended rate of pyridate in varying degrees. The symbiotic properties of chickpea cultivars were affected more than 10% at the recommended dose. The reduced nodulation ranged from 29% to 73% among cultivars exposed to pyridate at the recommended dose. The ‘Desi’ cultivar was more sensitive than the ‘Kabuli’ to the recommended rate of pyridate. We may conclude that effective low-use-rate of pyridate via applying of activator adjuvants should be noted.
Anatomical Study and Pollen Micromorphology of Onopordum L. in Iran
Authors: AGHABABAEYAN Elham , PAKRAVAN Maneezheh , GHAHREMANINEJAD Farrokh
Published: July 2019

In this study, pollen micromorphological structure, leaf and stem anatomy structure of six species of Onopordum (O. acanthium L., O. armenum Grossh., O. carduchorum Bornm. & Beauv., O. carmanicum (Bornm.) Bornm., O. heteracanthum C. A. Mey and O. leptolepis DC) were examined with different repetitions. About 17 qualitative anatomical features were statistically analyzed. Glandular trichome, cortex fiber tissue, embowed in subsidiary vascular, its fiber tissue and vascular sheath extensions, adaxial collenchyma tissue and types of adaxial and abaxial stomata were distinct characters in separating of the species. In palynologic studies based on P/E ratio, 2 types of pollen grains were observed: oblate- spheroidal and suboblate. SEM study of the exine showed echinate, reticulate and perforate sculpturing in all six species.
Introduction pages
Authors: SESTRAS Radu E
Published: July 2019

Introduction pages
Effect of Different Concentrations of IBA and Time of Taking Cutting on Rooting, Growth and Survival of Ficus binnendijkii ‘Amstel Queen’ Cuttings
Authors: BABAIE Hamed , ZAREI Hossein , NIKDEL Kosar , NAJAR FIROOZJAI Mostafa
Published: July 2019

In this research, in order to study the effect of different concentrations of IBA and time of taking cutting on rooting, growth and survival of F. binnendijkii ‘Amstel Queen’ cuttings, a test was conducted in a randomized complete block design with four replications. Treatments were consisted of four levels of hormones: 0 (control), 2000, 4000 and 6000 mg/l, with two intervals of time for taking cuttings (late June and early September). Results showed that highest root percentage, root number and fresh weight of root were for IBA concentrations of 4000 and 6000 mg/l and cuttings taken early in September. The maximum of mean length of root and longest root were achieved in concentration of 6000 mg/l IBA, taking cutting in early September. The highest survival percentage of plants was recorded in concentrations of 4000 and 6000 mg/l IBA, early September being the appropriate time of taking the cutting. The greatest length of new shoots was obtained in concentration of 4000 mg/l IBA, cutting in late June, while the maximum number of new leaf was in IBA concentration of 2000 and 4000 mg/l, with cuttings taken also in late June.