bluuebunny/crossref_metadata_2025 huggingface dataset:
SARS-CoV-2 Survival in Common Non-Alcoholic and Alcoholic Beverages
Authors: Jia Mo , Joyce Jonathan D. , Bertke Andrea S.
Published: March 2022

SARS-CoV-2, the causative agent of COVID-19, is known to be transmitted by respiratory droplets and aerosols. Since the virus is shed at high concentrations in respiratory secretions and saliva, SARS-CoV-2 would also be expected to be transmitted through activities that involve the transfer of saliva from one individual to another, such as kissing or sharing beverages. To assess the survival of infectious SARS-CoV-2 in common beverages, we quantified infectious virus by plaque assays one hour after inoculation into 18 non-alcoholic and 16 alcoholic beverages, plus saliva, and also 7 days later for 5 of these beverages. SARS-CoV-2 remains infectious with minimal reductions in several common beverages, including milk and beer. However, cocoa, coffee, tea, fruit juices, and wine contain antiviral compounds that inactivate SARS-CoV-2. Although hard liquors containing 40% alcohol immediately inactivate SARS-CoV-2, mixing with non-alcoholic beverages reduces the antiviral effects. In summary, SARS-CoV-2 can be recovered from commonly consumed beverages in a beverage type and time-dependent manner. Although aerosol or droplet transmission remains the most likely mode of transmission, our findings combined with others suggest that beverages contaminated with SARS-CoV-2 during handling, serving, or through sharing of drinks should be considered as a potential vehicle for virus transmission.
Mobility as a Service: Defining a Transport Utopia
Authors: Brown Clare , Hardman Michael , Davies Nick , Armitage Richard
Published: March 2022

Having been widely acknowledged as enabling access to education, employment, leisure and social activities, transport choices are also the cause of many challenges cities face. Recognising that change is needed, planners and policymakers are considering alternative methods of planning and delivering transport. Mobility as a Service (or MaaS) is one such idea that has gained traction with academics and professionals alike. Hailed as the answer to integrating complex transport systems, MaaS has yet to be implemented at scale in urban transport systems due in part to the lack of an agreed conceptual definition, the top-down approach to implementing what is meant to be a more personalised method of accessing transport, and the lack of local promoters (in comparison to global corporations and lobbyists). This article reflects on the current barriers to defining MaaS, considers how a novel public engagement approach could be used to create local definitions that support citizen engagement, and suggests a route forward for future research.
M 1–92 Revisited: New Findings and Open Questions: New NOEMA Observations of Minkowski’s Footprint
Authors: Alcolea Javier , Agúndez Marcelino , Bujarrabal Valentín , Castro-Carrizo Arancha , Desmurs Jean-François , Martínez-Fernández John-Eduard , Sánchez Contreras Carmen , Santander-García Miguel
Published: March 2022

PN M 1–92, also known as Minkowski’s Footprint, is a textbook example of a massive pre-planetary nebula. It presents all the characteristics of this type of source: non-spherical symmetry (bipolar cylindrical symmetry in this case), high-velocity gas emission, large amounts of linear momentum and kinetic energy (momentum excess), and a self-similar growing structure. We have revisited this object by performing new NOEMA observations (with half arc-second resolution) of a wealth of molecules, including the rare isotopologues of CO, as well as other less abundant species. These maps provide new insights into the origin of this source. Our findings include the discovery of molecular species in the ionised regions of the nebula, confirming its shocked origin; the structure of the massive equatorial component, including the presence of active wind collisions; and the strong evidence that the AGB evolution of the source was terminated prematurely, probably due to the huge mass loss event that resulted in the formation/acceleration of the present nebula.
Preservation of the Geoheritage and Mining Heritage of Serifos Island, Greece: Geotourism Perspectives in a Potential New Global Unesco Geopark
Authors: Vlachopoulos Nikolaos , Voudouris Panagiotis
Published: March 2022

Serifos island is characterized by rich geodiversity, industrial and cultural heritage. The present paper focuses on the geological and mining heritage of Serifos, with the aim of integrating the island in the international environment of Geoparks, in the near future. In this geopark, Serifos can highlight the rich geological heritage of the island combined with the rich industrial heritage as expressed by mining activities since prehistoric times and the mining facilities of iron and copper mines. During the present study, six geotrails have been developed to link these cultural and ecological sites with the geological heritage. Along the routes, the geodiversity is explained, including its relationship with the surrounding biodiversity, and the historical and cultural aspects of the region. In the proposed geocultural routes (geotrails), the dialectic relationship between Humans and Nature is determined by historical conditions and by the record of the process that transforms space into a landscape. The geological-mining heritage of Serifos will attract people from all over the world with different kind of interests and will make it known to alternative tourists. The results of this paper are intended to constitute a valuable tool for enhancing and raising awareness of the geological heritage of the island of Serifos.
The Fall Risk Screening Scale Is Suitable for Evaluating Adult Patient Fall
Authors: Chen Li-Chen , Shen Yung-Chao , Ho Lun-Hui , Shih Whei-Mei
Published: March 2022

(1) Background: This study aimed to test the feasibility of utilizing the screening tool for fall risk assessment in adult inpatient and verify its accuracy in a medical center in Taiwan. (2) Methods: This study retrospectively collected all adult fall cases among inpatients occurring in the general wards of a medical center between 1 January 2013 and 31 December 2015. This inpatient fall risk screening scale was measured by the sensitivity, specificity, and accuracy. (3) Results: There were 1331 (0.4%) falls among a total of 357,395 inpatients during this period. Factors predictive of falling risk included: age, consciousness, body shift assistance, use of fall risk medications, fall history, dizziness or weakness, toileting, and impaired mobility. Using the eight-factor assessment, two was the best cutoff point for identifying the fall risk group, with area under Receiver Operating Characteristic (ROC) curve (AUC) = 0.817, sensitivity = 80.93%, specificity = 73.0%, accuracy = 73.03%, and likelihood ratio = 11.48. (4) Conclusions: The accuracy of the eight-item fall risk assessment tool created for this study was validated. These results can serve as a reference for institutions to develop more effective fall risk assessment scale for inpatients, enabling clinical nurses to identify and more comprehensively assess the groups at highest risk for falling during their hospital stay.
The Longitudinal Dividing Bacterium Candidatus Thiosymbion Oneisti Has a Natural Temperature-Sensitive FtsZ Protein with Low GTPase Activity
Authors: Wang Jinglan , Bulgheresi Silvia , den Blaauwen Tanneke
Published: March 2022

FtsZ, the bacterial tubulin-homolog, plays a central role in cell division and polymerizes into a ring-like structure at midcell to coordinate other cell division proteins. The rod-shaped gamma-proteobacterium Candidatus Thiosymbion oneisti has a medial discontinuous ellipsoidal “Z-ring.” Ca. T. oneisti FtsZ shows temperature-sensitive characteristics when it is expressed in Escherichia coli, where it localizes at midcell. The overexpression of Ca. T. oneisti FtsZ interferes with cell division and results in filamentous cells. In addition, it forms ring- and barrel-like structures independently of E. coli FtsZ, which suggests that the difference in shape and size of the Ca. T. oneisti FtsZ ring is likely the result of its interaction with Z-ring organizing proteins. Similar to some temperature-sensitive alleles of E. coli FtsZ, Ca. T. oneisti FtsZ has a weak GTPase and does not polymerize in vitro. The temperature sensitivity of Ca. Thiosymbion oneisti FtsZ is likely an adaptation to the preferred temperature of less than 30 °C of its host, the nematode Laxus oneistus.
Intracavernous Injection of Platelet-Rich Plasma Therapy Enhances Erectile Function and Decreases the Mortality Rate in Streptozotocin-Induced Diabetic Rats
Authors: Liao Chun-Hou , Lee Kau-Han , Chung Shiu-Dong , Chen Kuo-Chiang , Praveen Rajneesh Chellappan , Chen Bo-He , Cheng Jai-Hong , Lin Wang-Ying , Chiang Han-Sun , Wu Yi-No
Published: March 2022

Erectile dysfunction (ED) is an agonizing complication of diabetes mellitus (DM) and it is challenging to treat ED in DM patients. Platelet-rich plasma (PRP) is a unique therapeutic strategy comprising intrinsic growth factors. An attempt was made to explore the potentiality of the PRP treatment in DM-induced ED rats in various groups (control, DM-non-ED, DM-ED, and DM-ED treated with PRP). Streptozotocin (STZ) was used to induce DM in rats. The blood glucose levels of the DM rats were maintained at >300 mg/dl. In the 18-week experiment, survival rate, body weight, intracavernous pressure (ICP) variations, and arterial blood pressure were analyzed. The tissue restoration results were validated by histological, immunofluorescence, and transmission electron microscopic analysis. PRP treatment of DM-ED rats significantly increased all parameters of erectile function compared to pre-treatment of PRP and DM-ED treated with vehicle. The histological results revealed that PRP treatment substantially enhanced the regeneration of myelinated nerves and decreased the atrophy of corporal smooth muscle. Notably, the PRP treatment immensely enhanced the survival rate in post-surgery DM-ED rats. These results indicated certain benefits of PRP treatment in delaying damage and preventing post-surgery complications in DM patients. Hence, PRP treatment is a novel multifactorial strategy for DM-ED patients.
Influence of Urea and Dimethyl Sulfoxide on K-Peptide Fibrillation
Authors: Wawer Jarosław , Karczewski Jakub , Aranowski Robert , Piątek Rafał , Augustin-Nowacka Danuta , Bruździak Piotr
Published: March 2022

Protein fibrillation leads to formation of amyloids—linear aggregates that are hallmarks of many serious diseases, including Alzheimer’s and Parkinson’s diseases. In this work, we investigate the fibrillation of a short peptide (K-peptide) from the amyloidogenic core of hen egg white lysozyme in the presence of dimethyl sulfoxide or urea. During the studies, a variety of spectroscopic methods were used: fluorescence spectroscopy and the Thioflavin T assay, circular dichroism, Fourier-transform infrared spectroscopy, optical density measurements, dynamic light scattering and intrinsic fluorescence. Additionally, the presence of amyloids was confirmed by atomic force microscopy. The obtained results show that the K-peptide is highly prone to form fibrillar aggregates. The measurements also confirm the weak impact of dimethyl sulfoxide on peptide fibrillation and distinct influence of urea. We believe that the K-peptide has higher amyloidogenic propensity than the whole protein, i.e., hen egg white lysozyme, most likely due to the lack of the first step of amyloidogenesis—partial unfolding of the native structure. Urea influences the second step of K-peptide amyloidogenesis, i.e., folding into amyloids.
Glucose Metabolism and Aging of Hematopoietic Stem and Progenitor Cells
Authors: Poisa-Beiro Laura , Landry Jonathan J. M. , Raffel Simon , Tanaka Motomu , Zaugg Judith , Gavin Anne-Claude , Ho Anthony D.
Published: March 2022

Comprehensive proteomics studies of human hematopoietic stem and progenitor cells (HSPC) have revealed that aging of the HSPC compartment is characterized by elevated glycolysis. This is in addition to deregulations found in murine transcriptomics studies, such as an increased differentiation bias towards the myeloid lineage, alterations in DNA repair, and a decrease in lymphoid development. The increase in glycolytic enzyme activity is caused by the expansion of a more glycolytic HSPC subset. We therefore developed a method to isolate HSPC into three distinct categories according to their glucose uptake (GU) levels, namely the GUhigh, GUinter and GUlow subsets. Single-cell transcriptomics studies showed that the GUhigh subset is highly enriched for HSPC with a differentiation bias towards myeloid lineages. Gene set enrichment analysis (GSEA) demonstrated that the gene sets for cell cycle arrest, senescence-associated secretory phenotype, and the anti-apoptosis and P53 pathways are significantly upregulated in the GUhigh population. With this series of studies, we have produced a comprehensive proteomics and single-cell transcriptomics atlas of molecular changes in human HSPC upon aging. Although many of the molecular deregulations are similar to those found in mice, there are significant differences. The most unique finding is the association of elevated central carbon metabolism with senescence. Due to the lack of specific markers, the isolation and collection of senescent cells have yet to be developed, especially for human HSPC. The GUhigh subset from the human HSPC compartment possesses all the transcriptome characteristics of senescence. This property may be exploited to accurately enrich, visualize, and trace senescence development in human bone marrow.
Effect of ALDH1A1 Gene Knockout on Drug Resistance in Paclitaxel and Topotecan Resistant Human Ovarian Cancer Cell Lines in 2D and 3D Model
Authors: Nowacka Marta , Ginter-Matuszewska Barbara , Świerczewska Monika , Sterzyńska Karolina , Nowicki Michał , Januchowski Radosław
Published: March 2022

Ovarian cancer is the most common cause of gynecological cancer death. Cancer Stem Cells (CSCs) characterized by drug transporters and extracellular matrix (ECM) molecules expression are responsible for drug resistance development. The goal of our study was to examine the role of aldehyde dehydrogenase 1A1 (ALDH1A1) expression in paclitaxel (PAC) and topotecan (TOP) resistant ovarian cancer cell lines. In both cell lines, we knocked out the ALDH1A1 gene using the CRISPR/Cas9 technique. Additionally, we derived an ALDH1A1 positive TOP-resistant cell line with ALDH1A1 expression in all cells via clonal selection. The effect of ALDH1A1 gene knockout or clonal selection on the expression of ALDH1A1, drug transporters (P-gp and BCRP), and ECM (COL3A1) was determined by Q-PCR, Western blot and immunofluorescence. Using MTT assay, we compared drug resistance in two-dimensional (2D) and three-dimensional (3D) cell culture conditions. We did not observe any effect of ALDH1A1 gene knockout on MDR1/P-gp expression and drug resistance in the PAC-resistant cell line. The knockout of ALDH1A1 in the TOP-resistant cell line resulted in a moderate decrease of BCRP and COL3A1 expression and weakened TOP resistance. The clonal selection of ALDH1A1 cells resulted in very strong downregulation of BCPR and COL3A1 expression and overexpression of MDR1/P-gp. This finally resulted in decreased resistance to TOP but increased resistance to PAC. All spheroids were more resistant than cells growing as monolayers, but the resistance mechanism differs. The spheroids’ resistance may result from the presence of cell zones with different proliferation paces, the density of the spheroid, ECM expression, and drug capacity to diffuse into the spheroid.
Exploiting Radiation Induction of Antigens in Cancer: Targeted Drug Delivery
Authors: Kapoor Vaishali , Singh Abhay K. , Lewis Calvin D. , Deore Sapna , Hallahan Dennis E.
Published: March 2022

Therapeutic antibodies used to treat cancer are effective in patients with advanced-stage disease. For example, antibodies that activate T-lymphocytes improve survival in many cancer subtypes. In addition, antibody–drug conjugates effectively target cytotoxic agents that are specific to cancer. This review discusses radiation-inducible antigens, which are stress-regulated proteins that are over-expressed in cancer. These inducible cell surface proteins become accessible to antibody binding during the cellular response to genotoxic stress. The lead antigens are induced in all histologic subtypes and nearly all advanced-stage cancers, but show little to no expression in normal tissues. Inducible antigens are exploited by using therapeutic antibodies that bind specifically to these stress-regulated proteins. Antibodies that bind to the inducible antigens GRP78 and TIP1 enhance the efficacy of radiotherapy in preclinical cancer models. The conjugation of cytotoxic drugs to the antibodies further improves cancer response. This review focuses on the use of radiotherapy to control the cancer-specific binding of therapeutic antibodies and antibody–drug conjugates.
Single-Cell RNA Sequencing with Spatial Transcriptomics of Cancer Tissues
Authors: Ahmed Rashid , Zaman Tariq , Chowdhury Farhan , Mraiche Fatima , Tariq Muhammad , Ahmad Irfan S. , Hasan Anwarul
Published: March 2022

Single-cell RNA sequencing (RNA-seq) techniques can perform analysis of transcriptome at the single-cell level and possess an unprecedented potential for exploring signatures involved in tumor development and progression. These techniques can perform sequence analysis of transcripts with a better resolution that could increase understanding of the cellular diversity found in the tumor microenvironment and how the cells interact with each other in complex heterogeneous cancerous tissues. Identifying the changes occurring in the genome and transcriptome in the spatial context is considered to increase knowledge of molecular factors fueling cancers. It may help develop better monitoring strategies and innovative approaches for cancer treatment. Recently, there has been a growing trend in the integration of RNA-seq techniques with contemporary omics technologies to study the tumor microenvironment. There has been a realization that this area of research has a huge scope of application in translational research. This review article presents an overview of various types of single-cell RNA-seq techniques used currently for analysis of cancer tissues, their pros and cons in bulk profiling of transcriptome, and recent advances in the techniques in exploring heterogeneity of various types of cancer tissues. Furthermore, we have highlighted the integration of single-cell RNA-seq techniques with other omics technologies for analysis of transcriptome in their spatial context, which is considered to revolutionize the understanding of tumor microenvironment.
Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling
Authors: Wang Mengying , Todorov Plamen , Wang Wanxue , Isachenko Evgenia , Rahimi Gohar , Mallmann Peter , Isachenko Vladimir
Published: March 2022

Introduction: Spermatozoa cryopreservation is an important technique to preserve fertility for males. This study aimed at exploring the stability of epigenetics information in human spermatozoa, manipulated by two different technologies, freezing and vitrification. Methods: Spermatozoa samples were distributed into three groups: 1. Fresh spermatozoa (control group), 2. Frozen spermatozoa, 3. Vitrified spermatozoa. Epigenetic differences of fresh and cryopreserved spermatozoa were evaluated using high-throughput RNA sequencing. Results: Differentially expressed genes (DEGs) in frozen (1103 genes) and vitrified (333 genes) spermatozoa were evaluated. The bioinformatical analysis identified 8 and 15 significant pathways in groups of frozen and vitrified spermatozoa, respectively. The majority of these pathways are most relevant to immune and infectious diseases. The DEGs of the fertilization process are not detected during vitrification. The freezing process induces more down-regulation of genes and is relevant to apoptosis changes and immune response. Conclusion: Cryopreservation of human spermatozoa is an epigenetically safe method for male fertility preservation. Cryoprotectant-free vitrification can induce more minor biological changes in human spermatozoa, in comparison with conventional freezing.
Angiogenic Imbalance and Inflammatory Biomarkers in the Prediction of Hypertension as Well as Obstetric and Perinatal Complications in Women with Gestational Diabetes Mellitus
Authors: Lara-Barea Almudena , Sánchez-Lechuga Begoña , Campos-Caro Antonio , Córdoba-Doña Juan Antonio , de la Varga-Martínez Raquel , Arroba Ana I. , Bugatto Fernando , Aguilar-Diosdado Manuel , López-Tinoco Cristina
Published: March 2022

Gestational diabetes mellitus (GDM) increases the risk of hypertensive disorders of pregnancy (HDP). We aimed to analyze the altered inflammatory markers and angiogenic factors among women with GDM to identify pregnant women at higher risk of developing HDP. Methods: This was a prospective study of 149 women without hypertension diagnosed in the third trimester with GDM. Inflammatory markers and angiogenic factors were measured at 28–32 weeks of pregnancy. Obstetric and perinatal outcomes were evaluated. Results: More than eight percent of the women developed HDP. Higher levels of the soluble fms-like tyrosine kinase-1/placental growth factor (sFlt-1/PIGF) ratio (4.9 ± 2.6 versus 2.3 ± 1.3, respectively; p < 0.001) and leptin (10.9 ± 0.8 versus 10.08 ± 1.1, respectively; p = 0.038), as well as lower levels of adiponectin (10.5 ± 1.3 versus 12.9 ± 2.7, respectively; p = 0.031), were seen in women who developed HDP versus normotensive women with GDM. A multivariable logistic regression analysis showed that adiponectin had a protective effect with 0.45-fold odds (0.23–0.83; p = 0.012), and that the sFlt-1/PIGF ratio was associated with 2.70-fold odds of developing HDP (CI 95%: 1.24–5.86; p = 0.012). Conclusion: An increase in angiogenic imbalance in the sFlt-1/PIGF ratio in women with GDM was detected and may be an indicator of developing HDP in addition to any subsequent obstetric and perinatal complications.
Recent Non-Invasive Parameters to Identify Subjects at High Risk of Sudden Cardiac Death
Authors: Corbo Maria Delia , Vitale Enrica , Pesolo Maurizio , Casavecchia Grazia , Gravina Matteo , Pellegrino Pierluigi , Brunetti Natale Daniele , Iacoviello Massimo
Published: March 2022

Cardiovascular diseases remain among the leading causes of death worldwide and sudden cardiac death (SCD) accounts for ~25% of these deaths. Despite its epidemiologic relevance, there are very few diagnostic strategies available useful to prevent SCD mainly focused on patients already affected by specific cardiovascular diseases. Unfortunately, most of these parameters exhibit poor positive predictive accuracy. Moreover, there is also a need to identify parameters to stratify the risk of SCD among otherwise healthy subjects. This review aims to provide an update on the most relevant non-invasive diagnostic features to identify patients at higher risk of developing malignant ventricular arrhythmias and SCD.
Value of CT-Textural Features and Volume-Based PET Parameters in Comparison to Serologic Markers for Response Prediction in Patients with Diffuse Large B-Cell Lymphoma Undergoing CD19-CAR-T Cell Therapy
Authors: Reinert Christian Philipp , Perl Regine Mariette , Faul Christoph , Lengerke Claudia , Nikolaou Konstantin , Dittmann Helmut , Bethge Wolfgang A. , Horger Marius
Published: March 2022

The goal of this study was to investigate the value of CT-textural features and volume-based PET parameters in comparison to serologic markers for response prediction in patients with diffuse large B-cell lymphoma (DLBCL) undergoing cluster of differentiation (CD19)-chimeric antigen receptor (CAR)-T cell therapy. We retrospectively analyzed the whole-body (WB)-metabolic tumor volume (MTV), the WB-total lesion glycolysis (TLG) and first order textural features derived from 18F-FDG-PET/CT, as well as serologic parameters (C-reactive protein [CRP] and lactate dehydrogenase [LDH], leucocytes) prior and after CAR-T cell therapy in 21 patients with DLBCL (57.7 ± 14.7 year; 7 female). Interleukin 6 (IL-6) and IL-2 receptor peaks were monitored after treatment onset and compared with patient outcome judged by follow-up 18F-FDG-PET/CT. In 12/21 patients (57%), complete remission (CR) was observed, whereas 9/21 patients (43%) showed partial remission (PR). At baseline, WB-MTV and WB-TLG were lower in patients achieving CR (35 ± 38 mL and 319 ± 362) compared to patients achieving PR (88 ± 110 mL and 1487 ± 2254; p < 0.05). The “entropy” proved lower (1.81 ± 0.09) and “uniformity” higher (0.33 ± 0.02) in patients with CR compared to PR (2.08 ± 0.22 and 0.28 ± 0.47; p < 0.05). Patients achieving CR had lower levels of CRP, LDH and leucocytes at baseline compared to patients achieving PR (p < 0.05). In the entire cohort, WB-MTV and WB-TLG decreased after therapy onset (p < 0.01) becoming not measurable in the CR-group. Leucocytes and CRP significantly dropped after therapy (p < 0.01). The IL-6 and IL-2R peaks after therapy were lower in patients with CR compared to PR (p > 0.05). In conclusion, volume-based PET parameters derived from PET/CT and CT-textural features have the potential to predict therapy response in patients with DLBCL undergoing CAR-T cell therapy.
Post-Resuscitation Partial Pressure of Arterial Carbon Dioxide and Outcome in Patients with Out-of-Hospital Cardiac Arrest: A Multicenter Retrospective Cohort Study
Authors: Okada Nobunaga , Matsuyama Tasuku , Okada Yohei , Okada Asami , Kandori Kenji , Nakajima Satoshi , Kitamura Tetsuhisa , Ohta Bon
Published: March 2022

We aimed to estimate the association between PaCO2 level in the patient after out-of-hospital cardiac arrest (OHCA) resuscitation with patient outcome based on a multicenter prospective cohort registry in Japan between June 2014 and December 2015. Based on the PaCO2 within 24 h after return of spontaneous circulation (ROSC), patients were divided into six groups as follows: severe hypocapnia (<25 mmHg), mild hypocapnia (25–35 mmHg,), normocapnia (35–45 mmHg), mild hypercapnia (45–55 mmHg), severe hypercapnia (>55 mmHg), or exposure to both hypocapnia and hypercapnia. Multivariate logistic regression analysis was conducted to calculate the adjusted odds ratios (aORs) and 95% confidence interval (CI) for the 1-month poor neurological outcome (Cerebral Performance Category ≥ 3). Among the 13,491 OHCA patients, 607 were included. Severe hypocapnia, mild hypocapnia, severe hypercapnia, and exposure to both hypocapnia and hypercapnia were associated with a higher rate of 1-month poor neurological outcome compared with mild hypercapnia (aORs 6.68 [95% CI 2.16–20.67], 2.56 [1.30–5.04], 2.62 [1.06–6.47], and 5.63 [2.21–14.34], respectively). There was no significant difference between the outcome of patients with normocapnia and mild hypercapnia. In conclusion, maintaining normocapnia and mild hypercapnia during the 24 h after ROSC was associated with better neurological outcomes than other PaCO2 abnormalities in this study.
Association of Hippocampal Subfield Volumes with Amyloid-Beta Deposition in Alzheimer’s Disease
Authors: Baek Min Seok , Lee Narae , Kim Jin Woo , Hong Jin Yong
Published: March 2022

We investigated the relationship between hippocampal subfield volumes and cortical amyloid-beta (Aβ) deposition in Alzheimer’s disease (AD). Fifty participants (11 cognitively unimpaired [CU], 10 with mild cognitive impairment [MCI], and 29 with AD) who underwent 18F-florbetaben positron emission tomography, magnetic resonance imaging, and neuropsychological tests were enrolled. The hippocampal subfield volumes were obtained using an automated brain volumetry system with the Winterburn atlas and were compared among the diagnostic groups, and the correlations with the Aβ deposition and AD risk factors were determined. Patients with MCI and AD showed decreased volume in the stratum radiatum/lacunosum/moleculare (SRLM) of the cornu ammonis (CA)1 and CA4-dentate gyrus (DG) compared with the CU. Decreased SRLM and CA4-DG volumes were associated with an increased Aβ deposition in the global cortex (R = −0.459, p = 0.001; R = −0.393, p = 0.005, respectively). The SRLM and CA4-DG volumes aided in the distinction of AD from CU (areas under the receiver operating characteristic [AUROC] curve = 0.994 and 0.981, respectively, p < 0.001), and Aβ+ from Aβ− individuals (AUROC curve = 0.949 and 0.958, respectively, p < 0.001). Hippocampal subfield volumes demonstrated potential as imaging biomarkers in the diagnosis and detection of AD and Aβ deposition, respectively.
Accelerated HF-rTMS Modifies SERT Availability in the Subgenual Anterior Cingulate Cortex: A Canine [11C]DASB Study on the Serotonergic System
Authors: Xu Yangfeng , Kappen Mitchel , Peremans Kathelijne , De Bundel Dimitri , Van Eeckhaut Ann , Van Laeken Nick , De Vos Filip , Dobbeleir Andre , Saunders Jimmy H. , Baeken Chris
Published: March 2022

Repetitive transcranial magnetic stimulation (rTMS) is thought to partly exert its antidepressant action through the serotonergic system. Accelerated rTMS may have the potential to result in similar but faster onset of clinical improvement compared to the classical daily rTMS protocols, but given that delayed clinical responses have been reported, the neurobiological effects of accelerated paradigms remain to be elucidated including on this neurotransmitter system. This sham-controlled study aimed to evaluate the effects of accelerated high frequency rTMS (aHF-rTMS) over the left frontal cortex on the serotonin transporter (SERT) in healthy beagle dogs. A total of twenty-two dogs were randomly divided into three unequal groups: five active stimulation sessions (five sessions in one day, n = 10), 20 active stimulation sessions (five sessions/day for four days, n = 8), and 20 sham stimulation sessions (five sessions/day for four days, n = 4). The SERT binding index (BI) was obtained at baseline, 24 h post stimulation protocol, one month, and three months post stimulation by a [11C]DASB PET scan. It was found that one day of active aHF-rTMS (five sessions) did not result in significant SERT BI changes at any time point. For the 20 sessions of active aHF-rTMS, one month after stimulation the SERT BI attenuated in the sgACC. No significant SERT BI changes were found after 20 sessions of sham aHF-rTMS. A total of four days of active aHF-rTMS modified sgACC SERT BI one month post-stimulation, explaining to some extent the delayed clinical effects of accelerated rTMS paradigms found in human psychopathologies.
Factors Influencing on Pain in Patients Undergoing Pipelle Endometrial Biopsy for Abnormal Uterine Bleeding: Why a Personalized Approach Should Be Applied?
Authors: Terzic Milan , Aimagambetova Gulzhanat , Ukybassova Talshyn , Bapayeva Gauri , Kaiyrlykyzy Aiym , Foster Faye , Linkov Faina
Published: March 2022

Objectives. Abnormal uterine bleeding (AUB) is a common complaint of women in different age groups, and endometrial biopsy is widely used to investigate the underlying causes. The aim of this observational study was to assess factors influencing pain in patients undergoing endometrial biopsy for AUB. Methods. Pain intensity before, during, and after Pipelle sampling was evaluated using the numerical rating scale (NRS), where “0” represents no pain at all, “10”—the worst pain ever possible. Pain rating was categorized as 1–6—mild to moderate, 7 and above as severe pain. Results. The study included 160 women who underwent Pipelle biopsy. The median age in the cohort was 42 (34–48) years, 18.1% of women were postmenopausal, 56.3% were either overweight or obese, 30% were nulliparous and 80% reported urban residency. The median pain score during the procedure was 2 (0–4). Pain scores of 5 (4–7) were reported with the junior gynecologist and 2 (0–4) in the senior gynecologist (p < 0.0001). Conclusion. The pain was found to have a strong association with the type of provider performing the endometrial sampling procedure. This fact suggests the need for a personalized approach and that psychological or informational interventions should be scheduled before the procedure to decrease pain and increase satisfaction.
Whole Brain Hemodynamic Response Based on Synchrony Analysis of Brain Signals for Effective Application of HD-tDCS in Stroke Patients: An fNIRS Study
Authors: Lee Gihyoun , Lee Jungsoo , Kim Jinuk , Kim Heegoo , Chang Won Hyuk , Kim Yun-Hee
Published: March 2022

In this study, the effective application of high-definition transcranial direct current stimulation (HD-tDCS) based on the whole brain hemodynamic response in stroke patients was investigated using functional near-infrared spectroscopy (fNIRS). The intrahemispheric and interhemispheric synchronization and cortical activity based on the time during 1 mA HD-tDCS were examined in 26 chronic cerebrovascular disease patients. At the beginning of HD-tDCS, the synchronization and brain activity in the whole brain increased rapidly and decreased after 5 min. In the middle of tDCS, the synchronization began to increase again, and strong synchronic connections were formed around the desired stimulation area. After tDCS, strong cortical activation was observed in the stimulation area, indicating that the baseline of the oxyhemoglobin (HbO) signal increased in the desired stimulation area. Therefore, the results of this study indicate that HD-tDCS can be applied efficiently to enhance the effect of tDCS. This stimulation method with tDCS can be explored clinically for more neurorehabilitation of patients with degenerative brain diseases.
Innovating Care in Multiple Sclerosis: Feasibility of Synchronous Internet-Based Teleconsultation for Longitudinal Clinical Monitoring
Authors: Sadeghi Nima , Eelen Piet , Nagels Guy , Cuvelier Corinne , Van Gils Katinka , D’hooghe Marie B. , Van Schependom Jeroen , D’haeseleer Miguel
Published: March 2022

The ‘coronavirus disease of 2019’ crisis has recently forced an expedited adoption of teleconsultation (TC) in most medical domains. Short-term digital interventions have generally been associated with feasibility, clinical benefits, user satisfaction, and cost-effectiveness in patients with multiple sclerosis (MS) but outcomes after repeated utilization over extended periods need to be further evaluated. In this feasibility study, 60 subjects with MS were 1:1 randomized to receive standard care augmented by four TCs using an audiovisual Internet platform (intervention) versus standard care alone (controls), over a period of 12 months. Effects on functional status, medical costs, and satisfaction were explored as secondary outcomes. Eighty-nine out of 108 scheduled TCs (82.4%) were completed, and 26 patients could complete at least one TC (86.7%), meeting our prespecified feasibility target of 80%. The intervention did not lead to significant differences in functional status (with the potential exception of fatigue) nor medical costs. Most interventional patients declared themselves to be (very) satisfied about the quality of care and technical aspects associated with the TCs. Our results demonstrate that longitudinal clinical monitoring using real-time audiovisual TC over the Internet is feasible and well-received by patients with MS. Such an approach can be a promising new care strategy.
Improved Music Perception after Music Therapy following Cochlear Implantation in the Elderly Population
Authors: Magele Astrid , Wirthner Bianca , Schoerg Philipp , Ploder Marlene , Sprinzl Georg Mathias
Published: March 2022

Background: Cochlear implantation (CI) and the accompanying rehabilitation has become a routine procedure in hearing restoration. Literature is sparse on elderly CI recipients focusing on the issue of age and their inclined auditory resolution, taking their diminished cognitive function into account, which requires adaptation of rehabilitation programs to overcome habituation. Objective: This study aims to show that a few adjustments in the therapy program towards age, mental, physical and auditory condition significantly improve music perception and overall auditory benefit, hence normal communication and social interactions can be found. Methods: Subjects implanted with a CI 65 years or older were compared to age-matched normal hearing subjects. Questionnaires were administered before and after ten music therapy sessions, to evaluate the participant’s music habits, the perception of sound quality and self-awareness and hearing implant satisfaction. Results: The greatest benefit was seen in participants’ gain in self-confidence and enjoyable music perception. Not only did the amount of listening to music increase, but also the impression of sound quality changed from poor up to good/very good sound quality. Conclusions: The music therapy was well accepted and resulted in beneficial subjective as well as objective outcomes towards hearing and music impression, hence improved quality of life.
Association of Warmer Weather and Infectious Complications Following Transrectal Ultrasound-Guided Prostate Biopsy
Authors: Chen Yu-Chen , Chen Hao-Wei , Huang Shu-Pin , Lin Szu-Huai , Chu Ting-Yin , Li Ching-Chia , Juan Yung-Shun , Wu Wen-Jeng
Published: March 2022

The seasonal and meteorological factors in predicting infections after urological interventions have not been systematically evaluated. This study aimed to determine the seasonality and the effects of the weather on the risk and severity of infectious complications (IC) after a transrectal ultrasound-guided prostate biopsy (TRUS-Bx). Using retrospectively collected data at the tertiary care hospital in Taiwan, we investigated the seasonal and meteorological differences in IC after TRUS-Bx. The IC included urinary tract infection (UTI), sepsis, and a positive culture finding (PCF). The severity was assessed on the basis of the Common Terminology Criteria for Adverse Events grading system. The prevalences of the infectious complications (UTI, sepsis, PCF and grade ≥ 3 IC) were significantly higher in the summer than in the winter. Monthly temperature and average humidity were significant factors for IC. After adjusting the demographic factors, multivariate regression revealed that UTI, sepsis, PCF, and grade ≥ 3 IC increased by 12.1%, 16.2%, 21.3%, and 18.6% for every 1 °C increase in the monthly average temperature, respectively (UTI: p = 0.010; sepsis: p = 0.046; PCF: p = 0.037; grade ≥ 3 IC: p = 0.021). In conclusion, the development and severity of IC after TRUS-Bx had significant seasonality. These were dose-dependently associated with warmer weather. Infectious signs after TRUS-Bx should be monitored more closely and actively during warm weather.
Multisource and Multilevel Investigations on a Historical Landslide: The 1907 Servigliano Earth Flow in Montemurro (Basilicata, Southern Italy)
Authors: Bentivenga Mario , Gizzi Fabrizio Terenzio , Palladino Giuseppe , Piccarreta Marco , Potenza Maria Rosaria , Perrone Angela , Bellanova Jessica , Calamita Giuseppe , Piscitelli Sabatino
Published: March 2022

Italy is one of the European countries most affected by landslides. In order to mitigate the risk, the analysis of such phenomena should involve a broad spectrum of studies to understand the geological and geomorphological properties of the unstable areas, the geometric features of the landslides and the causes of their trigger, the evolution over time, and the works of risk mitigation taken as well as their effectiveness over time. This article is concerned with multidisciplinary investigations on a historical earth flow occurred in Montemurro (Basilicata, Southern Italy) in 1907. We analyse unpublished archive sources strictly coupled with new geological and geomorphological surveys. Furthermore, to gain information on the geometrical features of the landslide body, geophysical prospections (ERT) is used alongside the field surveys. Lastly, to gain insight on the landslide triggering factors, we employed historical–climatological analysis: in particular, we made use of the monthly simple daily intensity index (SDII) to evaluate extreme events and the standardised precipitation index (SPI) to consider previous wetness conditions. The earth flow was triggered on 26 February 1907 and the main movement lasted about one week, involving several buildings, including those of cultural interest. Historical documentary investigations and historical climatological analysis both indicate that the earth flow was triggered by a preceding heavy rain period, which independent historical sources suggest also caused the activation of landslides over a wider area around Montemurro. Currently, the earth flow is NE–SW oriented, extends for a length of ~1.1 km, and has an average width of ~220 m. The landslide is in a dormant activity phase. From a methodological point of view, the research stresses the importance of integrated approaches to investigate natural hazards, particularly by the use of historical data. This research may be of interest to academics, practitioners, and policymakers for both the methodological approach followed and results gained, useful in view of both risk mitigation and territorial planning of landslide-prone areas.
Using Land to Promote Refugee Self-Reliance in Uganda
Authors: Berke Timothy , Larsen Larissa
Published: March 2022

Around the world, the number of people displaced from their homes continues to increase due to political conflict and climate change. The Ugandan government’s policy for settling refugees shifts the focus from seeing refugees as humanitarian obligations to recognizing that refugee settlements bring improved services and infrastructure that can serve both refugees and residents of the host communities. A key aspect of this policy is to provide refugees with land use rights, so they are able to achieve “self-reliance”. This research investigates (1) the role of planning in establishing the physical layout of Bidi Bidi, Uganda’s largest integrated settlement, (2) the host community’s rationale for allowing refugees access to their land, and (3) how refugees used this land. Based on interviews, participant observation, GIS analysis, and document analysis, we learned how refugees altered the settlement’s layout to better meet their needs, the unintended consequences of large- scale deforestation on the most vulnerable, and the limitations of the land-based approach to achieve self-reliance. These findings suggest expanding the avenues to achieve self-reliance, improving “integration” of service provision between refugees and residents of the host community, and protecting the area’s environment to maintain needed resources.
From Vulnerability to Empowerment: Critical Reflections on Canada’s Engagement with Refugee Policy
Authors: Klassen Amanda
Published: March 2022

The making and implementation of global policy are prominent areas of activity for the global refugee regime, with a specific focus on policy relating to the categories of vulnerable refugees. Recent collective efforts globally have highlighted the importance of meaningfully including refugees themselves; and a discursive shift away from the language of vulnerability towards that of empowerment in policy making, and humanitarian assistance. Despite this, efforts to implement these commitments have largely been unsuccessful, raising questions about how refugees are engaged in these processes, and in what ways the label of vulnerable continues to influence the making and implementation of global refugee policy. Using the case of Canada’s engagement with the global refugee regime, and with refugee women in particular, this article argues that the continued framing of refugee women as vulnerable has impeded progress, and that for transformative policy to be realized, refugee women must be seen as actors with capacity to participate, and must be included in all processes of policy making, implementation and evaluation. A feminist geopolitical framework is presented as a way to decenter states and institutions in favor of centering the individual embodied experiences of refugee women in global refugee policy making. By doing so, empowerment can be realized in policy and practice.
New OSCE Recommendations to Combat Corruption, Money Laundering, and the Financing of Terrorism
Authors: Yeh Stuart S.
Published: March 2022

A model Anticorruption Protocol to the United Nations Convention against Corruption (APUNCAC) would implement international requirements to report the beneficial ownership of funds involved in certain financial transactions. The purpose is to discourage the laundering of illicit funds by attaching legal consequences to each failure to obtain and submit a required report of beneficial ownership, and each failure by a front man who poses as a beneficial owner to supply true information regarding the identity of the actual beneficial owner. This article is the fifth in a series of articles describing APUNCAC’s anti-money-laundering (AML) provisions and focus on beneficial owner transparency. The companion articles focus on issues regarding international jurisdiction and enforcement of APUNCAC regarding distant offshore personnel, illustrate the application of APUNCAC to specific money laundering channels, answer frequently asked questions (FAQ), and translate APUNCAC’s key provisions into proposed Financial Action Task Force (FATF) recommendations. This article explains how APUNCAC’s key provisions may be translated into Organization for Security and Co-operation in Europe (OSCE) recommendations, why the OSCE may be especially inclined to adopt the recommendations, and why support of this initiative might be the most promising path that could be adopted by the international community to combat corruption, money laundering, and the violations of human rights that are associated with these crimes.
Genetic Landscape of Peripheral T-Cell Lymphoma
Authors: Hathuc Vivian , Kreisel Friederike
Published: March 2022

Peripheral T-Cell lymphoma (PTCL) comprises a heterogenous group of uncommon lymphomas derived from mature, post-thymic or “peripheral” T- and natural killer cells. The World Health Organization (WHO) emphasizes a multiparameter approach in the diagnosis and subclassification of these neoplasms, integrating clinical, morphologic, immunophenotypic, and genetic features into the final diagnosis. Clinical presentation is particularly important due to histologic, immunophenotypic and genetic variations within established subtypes, and no convenient immunophenotypic marker of monoclonality exists. In recent years, widespread use of gene expression profiling and next-generation sequencing (NGS) techniques have contributed to an improved understanding of the pathobiology in PTCLs, and these have been incorporated into the 2016 revised WHO classification of mature T- and NK-cell neoplasms which now encompasses nearly 30 distinct entities. This review discusses the genetic landscape of PTCL and its role in subclassification, prognosis, and potential targeted therapy. In addition to discussing T-Cell lymphoma subtypes with relatively well-defined or relevant genetic aberrancies, special attention is given to genetic advances in T-Cell lymphomas of T follicular helper cell (TFH) origin, highlighting genetic overlaps between angioimmunoblastic T-Cell lymphoma (AITL), follicular T-Cell lymphoma, and nodal peripheral T-Cell lymphoma with a TFH phenotype. Furthermore, genetic drivers will be discussed for ALK-negative anaplastic large cell lymphomas and their role in differentiating these from CD30+ peripheral T-Cell lymphoma, not otherwise specified (NOS) and primary cutaneous anaplastic large cell lymphoma. Lastly, a closer look is given to genetic pathways in peripheral T-Cell lymphoma, NOS, which may guide in teasing out more specific entities in a group of T-Cell lymphomas that represents the most common subcategory and is sometimes referred to as a “wastebasket” category.