bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and selective Cu(II) complexation of lower rim substituted thiacalixarenes containing pyrazole fragments
Authors: Kalinin Valeriy , Rasperetikhina Ekaterina , Bukharov Mikhail , Shibaeva Ksenia , Islamov Daut , Babaeva Olga , Stoikov Ivan
Published: December 2024

Selective sensors for the detection and quantification of Cu(II) ions are actively used to investigate in vivo conditions in Wilsons and Menkes diseases at the cellular level. Such sensors must have a high specificity for this cation as well as low toxicity. A synthetic approach for the preparation of selective sensors for Cu(II) cations based on p-tert-butylthiacalix[4]arenes modified with 3,5-dimethylpyrazole moieties in cone, partial cone and 1,3-alternate stereoisomeric forms was developed. The obtained compounds were characterized by single-crystal XRD, NMR and IR spectroscopy, MALDI MS, and elemental analysis. All synthesized thiacalixarene compounds formed complexes with Cu(II) with the binding constants in range logK = 4.44–4.63 and 1:1 stoichiometry. The structure of the obtained complexes was studied by NMR spectroscopy and DFT methods. The obtained results can be used to develop selective Cu(II) sensors.
Unveiling the dynamics of climate change narratives: A Google Trends analysis
Authors: Erokhin Dmitry , Komendantova Nadejda
Published: December 2024

This study explores public interest and misinformation surrounding climate change through an analysis of Google Trends data. It focuses on online search behaviors related to “climate”, “climate change”, “climate hoax”, and “HAARP” throughout 2023. By tracking search patterns, we aim to understand public perceptions, concerns, and the influence of misinformation on climate change discourse. Our findings indicate a strong temporal and regional variability in search interest, which spikes notably during significant events like Earth Day and in response to public statements by influential figures. The results demonstrate high engagement in regions with direct climate impact, such as Africa and parts of Asia, contrasted with varied engagement in Western nations. Notably, the data reveals significant public intrigue following conspiracy narratives during specific periods, which emphasizes the role of misinformation in shaping public discourse. This analysis underscores the need for targeted communication strategies to effectively address public misconceptions and enhance the understanding of climate-related issues. This research highlights the utility of Google Trends as a tool for real-time monitoring of public interest and misinformation and offers valuable insights for policymakers, educators, and activists to tailor their approaches to combat climate change more effectively. Through its comprehensive analysis, the study contributes to the broader understanding of how digital tools can aid in the ongoing efforts to engage the public in meaningful climate action discussions.
Functional Analysis of VOX's Climate Crisis Discourse on Twitter/X
Authors: Vicente Torrico David , Díez Garrido María
Published: December 2024

The climate crisis stands as one of the foremost threats to humanity, demanding urgent measures to reduce greenhouse gas emissions. Despite its critical nature, climate policy has become a contentious issue, intensified by ineffective communication strategies and polarized public discourse. Social media platforms have become significant arenas for opinion formation, often exacerbating misinformation and public harassment. This research employs the Functional Theory of Discourse to analyze on the Spanish far-right party Vox's discourse on the social media platform X (formerly Twitter) regarding environmental policies. A total of 91 posts from Vox's official account and its leader, Santiago Abascal, were analyzed over six months, from September 2022 to February 2023. The posts were categorized based on their discursive functions: acclaims, attacks, and defenses, with further subcategorization into policy and character appeals. The analysis reveals that Vox's communication strategy balances attacks and acclaims. Attacks primarily targeted the Spanish government and international bodies like the European Union, using themes of dishonesty, incompetence and opposition to climate measures. Acclaims highlighted Vox's proposals for energy sovereignty, opposition to the Climate Change and Energy Transition Law and leadership qualities. Therefore, Vox’s discourse on environmental policies is characterized by simplicity and a dual focus on attacks and acclaims. Their messaging promotes nationalistic and conservative ideals, positioning themselves against progressive and environmentalist ideologies. This strategy of polarization and confrontation, aided by multimedia elements, effectively engages their audience but poses challenges for democratic debate and the implementation of effective climate policies. Future research should explore the implications of such discursive strategies on public opinion and policy-making.
Anti- and pro-environmental disinformation. Fact-checking Spanish political parties’ discourse
Authors: Fenoll Vicente , Alonso-del-Barrio Estrella , Gamir-Ríos José
Published: December 2024

Climate disinformation’s studies focus mainly on denialism or false discourses justifying inaction. However, pro-environmental disinformation is also possible, exaggerating climate change consequences or green policies benefits. Anti- and pro-environmental disinformation can negatively influence citizens’ political decision-making. The aim of this research is to analyze the disinformation coming from Spanish political actors, to check what issues they focus on and if there is an ideological bias in the dissemination of false information related to climate change and the environment. The investigation is based on verifications carried out by fact-checkers Newtral and Maldita on statements made by Spanish politicians. From a quantitative perspective, all fact-checks performed from October 2018 to March 2023 are analyzed (N = 1,415). The study covers a time frame of almost five years, which includes elections at European and national level. We classified each disinformation according to date, source, and topic, and we make a taxonomic proposal of environmental disinformation, be it anti- or pro-environmental action. We aim to find out what kind of environmental disinformation is used in Spain to cover the lack of studies in this field. Also to check the differences in the disinformation strategy of the parties, especially in the radical right. Results reveal for the first time the presence of pro-environmental disinformation. However, disinformation is mostly anti-environmental and occupies a low level within the disinformation strategy of the parties, which reflects the low interest in this issue. Findings show that denialism is on the decline. Instead, anti-environmentalism has become more pragmatic and prevails through inactivism. Conservative parties lead in environmental disinformation, although it is more frequent in the more moderate ones. There is also an increase in this type of disinformation before the European election, which suggests an instrumental use at the political level where the environment is perceived to be more relevant.
Le Pen, Zemmour and the ‘patriotic ecology’ of the French far right: The case of the 2022 presidential elections
Authors: Aiolfi Theo
Published: December 2024

The French far right has historically been torn between two strategies which were opposed during the 2022 presidential campaign, a modernist line who was embodied by Marine Le Pen’s Rassemblement National and a traditionalist line who was represented by Éric Zemmour’s Reconquête. This article specifically examines the way the differences between the modernist and traditionalist lines were expressed when it comes to the topic of environmental concerns. Indeed, while ecological issues have long been seen as the exclusive domain of the political left, the increasing salience of the climate crisis means that politicians across the political spectrum have provided their own perspective on ecological issues. Building on a deeper tradition of conservative and nationalist thought about the environment, this article demonstrates that the French far right has developed a form ‘patriotic ecology’ that can be interpreted in different ways. Through an analysis of their programmes for the 2022 presidential campaign, the article contrasts the archetypical way Zemmour mobilised environmental issues by mobilising the classic far-right tropes about the environment while Le Pen engaged with them in much more modern and sophisticated way to depict herself as more in touch with the contemporary zeitgeist. Finally, it concludes with a discussion on the ideological plasticity of the far right and the need to address its versatility in appropriating issues beyond its ideological core.
O IMAGINÁRIO PATAXÓ NA KIJĒTXAWÊ TXAÊAÔ TXIHIHÃE PATAXÓ ŪG HÃ HÃ HÃE KATURÃMA
Authors: Neves Martins Juliana , Noronha Vânia
Published: December 2024

Esse artigo é fruto de uma pesquisa realizada com o objetivo de identificar o imaginário nas experiências cotidianas e coletivas do povo Pataxó e Pataxó Hã Hã Hãe, em especial, em relação aos mitos, e, ainda, analisar como estes são trabalhados na educação escolar indígena da Aldeia Katurãma. A pesquisa foi realizada na comunidade indígena que hoje reside em São Joaquim de Bicas/MG, com a adoção da história oral, por meio da tradição oral, para a coleta de dados. Foram realizadas entrevistas com sete professores, além da observação participante, registrada em um diário de campo. Desse modo, foram destacados, pelos sujeitos da pesquisa, cinco mitos, nos quais pudemos identificar as imagens centrais, posteriormente analisadas com base na teoria do imaginário de Durand (2002). Conclui-se que os mitos são parte integrante da cosmovisão dessas etnias e suas imagens míticas, presentes em suas práticas educativas, na escola e em outras dimensões da vida, contribuem para a manutenção da cultura, da luta e da resistência desses povos.
A DIDÁTICA CRÍTICA INTERCULTURAL: REFLEXÕES A PARTIR DOS PLANOS DE ENSINO
Authors: Abreu Roberta , D'Ávila Cristina Maria
Published: December 2024

Este artigo tem como objetivos analisar se, e como a perspectiva da interculturalidade está presente nos planos de ensino do componente Didática nos cursos de Licenciatura em Pedagogia de algumas instituições de ensino superior do Nordeste brasileiro; identificar como a interculturalidade se faz presente nos objetivos e conteúdos programáticos desses mesmos planos. Utilizou-se a análise documental como metodologia de pesquisa chegando aos seguintes resultados: tanto nos objetivos como nos conteúdos, a referida perspectiva não se mostrou de forma recorrente. Ressalta-se que as relações da Didática com a interculturalidade são uma construção recente e ainda se encontram numa fase de internalização/ressignificação em pensamento/ações do ensinar. Há necessidade de sua implementação fomentando uma leitura outra dos dinamismos históricos e da realidade.
Impacts of Mg/Al-layered double hydroxide modified with polycarboxylate on the properties of cement paste
Authors: Chen Juan , He Yan , Jiang Mingjing
Published: December 2024

Mg/Al-layered double hydroxide (Mg/Al-LDH) has been claimed as a potential hardening accelerator of cementitious materials. The performance of Mg/Al-LDH modified with polycarboxylate (PCE) superplasticizer incorporated into cementitious materials is vitally important, and needs to be completely understood. In this paper, four kinds of Mg/Al-LDH-PCE are incorporated into cement paste. The rheological performance and hydration process of the cement paste are analyzed. The results show that LDH-PCE is advantageous for modifying the rheological performance of fresh cement paste. With increasing content of LDH-PCE, the viscosity and the yield stress of fresh binder decrease gradually. Also, LDH3-PCE contributes to the lowest yield stress. The adoption of LDH-PCE prolongs the introduction period of cement paste, but contributes to an elevated exothermic peak in the acceleration period and enhances cumulative hydration heat flow, indicating enhanced hydration of silicates. Owing to the enhanced cement hydration with the adoption of LDH-PCE, more hydration products form in the matrix, and contribute to improved compressive strength. Among the four kinds of LDH-PCEs, the application of LDH3-PCE leads to the highest compressive strength of cement mortar at 28 days, with a 15.6% increment compared with the blank sample.
Development of a prototype bridge scour sensor exploiting vortex-induced vibrations
Authors: Campbell Kristopher , Lydon Myra , Taylor Su , Hamill Gerard , O’Higgins Connor , Prendergast Luke J. , O’Connor Alan , Nieuwland Remco
Published: December 2024

The ability to effectively manage the deterioration of a network-wide bridge stock condition from year to year is vital for the effective use of finite resources while maintaining public safety. The leading cause of bridge failures and deterioration globally is bed scour. This paper looks at the development of a novel fibre Bragg grating (FBG) vortex flow sensor (VFS) for scour, utilising vortex-induced vibration flow effects. These sensors can provide early warnings to bridge managers and allow for a proactive approach to scour management at vulnerable bridges. This paper briefly reviews the current types of scour sensors available before outlining the initial development of the novel VFS set-up and associated prototype development. The initial findings of laboratory trials are presented before outlining the selection process for a masonry arch bridge test site.
BİR UZMANLIK ALANI OLARAK TARIM HABERCİLİĞİ: GAZETELER VE HABER SİTELERİ ÜZERİNE BİR ARAŞTIRMA
Authors: Akyazı Ayşenur
Published: December 2024

Küresel anlamda gıdaya erişimin giderek zorlaştığı günümüzde, tarım alanında yaşanan değişim, dönüşüm, gelişmeleri veya sorunları halka duyurmak için bir uzmanlık alanı olarak tarım haberciliği önem kazanmaktadır. Çalışma kapsamında doküman analizi yöntemi kullanılarak 07.09.2023-16.09.2023 tarihleri arasında 10 adet gazete ve 10 adet haber sitesinin yaşam, gündem, güncel, ekonomi ve diğer sekmeleri altında yer alan tarım haberlerine erişilmiştir. Ayrıca bu haberlerin ilgili medya kuruluşunun X (Twitter) hesabından paylaşılıp paylaşılmadığı bilgisine ve paylaşımların aldığı etkileşim sayılarına ulaşılmıştır. Çalışmada medya kuruluşlarının yanı sıra halkın bu tür haberlere verdiği önemin ortaya konulması amaçlanmıştır. Yapılan analiz ile insanlık için giderek çok daha fazla önemli hale gelmekte olan tarım haberlerine medya kuruluşlarının yok denecek kadar az yer verdiği, toplumun da gerekli ilgiyi göstermediği sonucuna ulaşılmıştır.
GAMIFICATION IN SUSTAINABILITY LEARNING IN BUSINESS EDUCATION
Authors: Özgen Özge , Demirel Banu
Published: December 2024

This study was conducted on gamification, sustainability learning, and business education dimensions. The main objective of this study is to synthesize the empirical evidence already available to gain a comprehensive understanding of how gamification can enhance sustainability learning in business education. Since integrating these three dimensions is a new and emerging area in the literature, early work in this field is expected to contribute significantly to future studies. According to the content analysis, the primary finding of the analyzed studies is that they mainly emphasize the cognitive engagement aspect. However, when the sustainability dimension is incorporated into gamification in business education, the games become excessively complex. Moreover, the studies examining cognitive outcomes need to adequately consider students' emotional states, and more attention is given to the behavior change associated with sustainability.
Book review: Chris Pallant and Christopher Holliday (eds.), Snow White and the Seven Dwarfs: New Perspectives on Production, Reception, Legacy.
Authors: Archer Neil
Published: December 2024

Chris Pallant and Christopher Holliday (eds.), Snow White and the Seven Dwarfs: New Perspectives on Production, Reception, Legacy. (London and New York: Bloomsbury, 2022), pp. 328, ISBN:  9781501373961, pbk. £28.99.
Identificación de rasgos básicos en el diseño de logotipos: el caso de las entidades de la Federación de ONG Caldas
Authors: Montes Vozmediano Manuel , Vizcaíno Laorga Ricardo , Gallego Gómez Juan Diego
Published: December 2024

El presente estudio tiene como objetivo principal comprobar si los logotipos de las ONG colombianas cumplen los criterios básicos de este tipo de representaciones de imagen de marca. A través del análisis de 35 variables (manifiestas y latentes) aplicado a los logotipos de las entidades de la Federación de ONG de Caldas se identifican elementos que no deberían estar presentes en productos profesionales (desequilibrio entre elementos textuales y cantidad de colores utilizados; casos de baja inteligibilidad y legibilidad; exceso de detalles irreproducibles a determinados tamaños). Se observa una baja presencia de elementos de identificación cultural con el contexto colombiano o de Caldas. Se comprueba un uso predominante del texto como elemento para dejar constancia del desempeño o labor social llevado a cabo por la ONG, mientras que dos terceras partes de los logotipos utilizan la imagen para trasladar asociaciones con la actividad que realiza la entidad, con los destinatarios de la misma o con la labor de servicio (entendida esta de manera genérica). Se detectan algunos usos estereotipados de la imagen y los colores (especialmente de género). La tendencia observada es la inclusión de uno o dos epígrafes textuales jerarquizados mediante diferentes escalas, el empleo de dos gamas cromáticas de referencia (los tonos fríos y los acromáticos), el uso de imágenes figurativas no realistas (siluetas), tipografías de palo seco y la inserción de grafismos (filetes o fondos) con diversas funcionalidades.
LegalTech in Digital Economy and in Legal Regulation of Individuals Economic Activities
Authors: Savenko Natalya
Published: December 2024

Based on law and doctrine, the article examines the categories of ‘digital economy’,‘LegalTech’ and ‘individuals’ economic activities’ in their interaction. It stresses that those categories represent Russia’s priority lines of development and can be fully digitalised. Legal science reflects diverse interpretations of LegalTech. There is a widespread understanding is that LegalTech is a narrow toolkit for lawyers. The author argues for an expansive interpretation of LegalTech as a comprehensive phenomenon intended for a wide range of economic agents, and shows LegalTech to be both anelement of the digital economy and a digitalised means for legal regulation of individuals’ economic activities. Trends and risks in the implementation and use of LegalTechtools are identified. In the aspect of legal regulation, the functional characteristics of LegalTech are formulated on the basis of an instrumental legal approach.
Experiència d’ús d’una Simulació Virtual Gamificada per a docents d’Infermeria
Authors: Baeza González Adrián , Usart Rodríguez Mireia , Marqués Molías Luis
Published: December 2024

Les simulacions virtuals han emergit com a valuoses eines educatives que recreen escenaris en els quals els alumnes poden practicar els seus coneixements en un entorn segur i controlat. Aquestes simulacions, a més de ser valorades per la seva facilitat d’ús i capacitat motivadora, milloren el coneixement i la confiança dels estudiants.
L’objectiu d’aquest treball és comprendre en profunditat l’experiència d’ús de la simulació virtual “Hello, you must be Flo!” per part de docents universitaris d’Infermeria, i identificar el seu nivell de coneixement en les àrees del TPACK, els desafiaments que enfronten, així com les emocions i motivacions que senten. Per a això es va adoptar un enfocament qualitatiu interpretatiu, basat en entrevistes semiestructurades realitzades abans i després de la implementació de la simulació.
Els resultats mostren un nivell de coneixement tecnològic i pedagògic més limitat en comparació amb el seu coneixement del contingut, on mostren una seguretat més gran. Això deriva, en primer lloc, en una inconsistència procedimental a l’hora d’aplicar la simulació a l’aula. En segon lloc, en una afectació en els seus nivells de confiança i en una sèrie de desafiaments que han d’afrontar, entre els quals destaquen l’organització de l’activitat a l’aula i la falta de contextualització en la programació didàctica.
Els resultats conclouen en la utilitat de la guia d’implementació per suplir les necessitats dels docents de cara a implementar la simulació amb l’alumnat, augmentar el seu grau de confiança i aconseguir una aplicació més estructurada, completa i coherent de l’eina.
Feminist Theory Theater: Acts of Reading as Embodied Pedagogy
Authors: Aushana Christina , Berman Michael , Gluzman Yelena , Klein Sarah
Published: December 2024

This article introduces Feminist Theory Theater (FTT), an experimental reading practice developed by the co-authors. Described most simply, FTT asks a group of co-present readers to put a text “on its feet,” improvising and revising its performance as a mode of ongoing, embodied interpretation. The aim is not to settle on a consensus of what a text means or to work toward a finished performance. Instead of staging a single best performance, FTT invites texts-as-scenes to be interpreted and re-staged by any member(s) of the reading group. We offer FTT as a way to take up York and Conley’s (2019) proposal that the commitments of STS can and should be enacted in practices of pedagogy. Here, we present and analyze multiple scenes of FTT in action to consider the potentials and limitations of critical STS pedagogy. We include our earliest experiments developing FTT in Act I, reading Judith Butler with undergraduates in a university lecture hall in Act 2, and reading a syllabus with incarcerated students in a prison classroom in Act 3. We highlight the empirical ways that FTT resists interpretive closure, centering embodied reinterpretation, arguing that doing so re-embeds text in the world as a way for reading groups to revision both. However, this dynamic, non-teleological mode of reading causes trouble for lesson plans and “learning outcomes” that might support the institutional legitimacy of STS critical pedagogies. This contradiction hinges on the question of who and what teaches. We argue that this trouble is worth staying with as a practical contradiction to be grappled with in further research on and through STS critical pedagogies. We invite readers of this article to take up this question (and others) by trying with, reflecting on, and revising through the situated, open-ended mode of reading together that we call FTT. To that end, we present a free, printable zine, The Feminist Theory Theater Workbook, which can act as both a guide to a first attempt at doing FTT and an archivable trace of that reading.
Results of surgical treatment of unilateral and bilateral otosclerosis in children
Authors: Pastuszak Andrzej , Skarżyński Henryk , Gos Elżbieta , Kołodziejak Aleksandra , Plichta Łukasz , Skarżyński Piotr H.
Published: December 2024

Wprowadzenie

Otoskleroza jest chorobą wieloczynnikową, w której dochodzi do nieprawidłowej przebudowy kostnej błędnika powodującej niedosłuch. Może występować zarówno u dorosłych, jak i u dzieci. Zazwyczaj występuje obustronnie, a w ok. 15% przypadków – jednostronnie.

Materiał i metody

Do badania włączono 9 dzieci. Materiał zaprezentowano w podziale na otosklerozę jednostronną i obustronną. U dzieci wykonano stapedotomię z użyciem mikrowiertarki. Do operacji użyto protezki Mikołów III BO oraz Skarzynski Piston Titanium. Tłok protezki Skarzynski Piston Titanium wykonany jest z czystego tytanu (ASTM F67). Następnie porównano wyniki audiometryczne wykonane: przedoperacyjnie, 2 oraz 12 miesięcy po operacji.

Wyniki

U dzieci z rozpoznaną otosklerozą jednostronną 12 miesięcy po operacji zamknięcie rezerwy (ABG do −10 dB) było obserwowane we wszystkich uszach. Natomiast u dzieci z rozpoznaną otosklerozą obustronną 12 miesięcy po operacji zamknięcie rezerwy stwierdzono w 88,9% uszu.

Wnioski

Stapedotomia w przypadku młodzieńczej otosklerozy jest bezpieczną i skuteczną procedurą chirurgiczną możliwą do wykonania u dzieci.
Hi-C protocol v1
Authors: Luo Renhe , Beer Michael , Huangfu Danwei
Published: December 2024

Hi-C protocol for ESC DE differentiation time course.
siRNA-mediated Knockdown – RAW 264.7 v1
Authors: Bentley-DeSousa Amanda , Ferguson Shawn
Published: December 2024

This protocol details steps taken to perform siRNA-mediated knockdowns in RAW 264.7 cells.
USDA LTAR Common Experiment measurement: Dissolved nitrate (NO3-) concentration v1
Authors: Lizotte Richard
Published: December 2024

Nitrate (NO3-) is an ionized form of nitrogen (N) that is of interest in surface and groundwater. Nitrate often occurs in trace amounts (<0.01 mg/L) in rivers and lakes but can reach high levels in some surface water and groundwater. In excessive amounts, it can elicit an illness known as methemoglobinemia in infants, and the drinking water standard is limited to 10 mg NO3--N/L to prevent this illness. In addition, NO3- is an essential nutrient for growth and photosynthesis in aquatic autotrophs, and elevated NO3- loading contributes to eutrophication and hypoxia of surface waters, particularly in coastal marine ecosystems. The recommended methods for measuring NO3- concentration in water include the colorimetric cadmium reduction method, whereby NO3- is reduced quantitatively to nitrite (NO2-) in the presence of cadmium. Following the addition of appropriate reagents, a highly colored azo dye is produced and measured colorimetrically. An alternative enzymatic method utilizes “greener” chemistry by replacing the toxic copperized cadmium with nitrate reductase (specifically, AtNaR2) in combination with two coenzymes (nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate) to reduce NO3- to NO2-, followed by the identical colorimetric quantification reaction used in the cadmium method. This enzyme reduction method overcomes interferences from phenolics and humic substances noted in earlier enzymatic methods. The method detection levels (or limits; MDL) for standard and low NO3- + NO2- concentrations with enzyme reduction are comparable to the long-term MDLs of the continuous-flow analyzer method with cadmium reduction. In addition, NO3- concentration differences were statistically equivalent to zero or analytically equivalent to zero (i.e., concentration differences were less than MDLs [Patton and Kryskalla, 2011]). Recent advances in field sensor technology have afforded commercial sensors that allow for in situ NO3- measurement using an optical sensor. These sensors can record values over short durations (seconds to minutes) and use an ultraviolet spectrophotometer (Johnson and Coletti, 2002). Although these sensors provide high-resolution data, their accuracy can degrade because of background interference from colored dissolved organic matter (DOM) and high turbidity. Thus, sensor readings must be validated at least monthly by conducting water sample collection and lab analyses.
Perturb-seq protocol v1
Authors: Luo Renhe , Beer Michael , Huangfu Danwei
Published: December 2024

Perturb-seq protocol for ESC DE differentiation
Genome Edited (KO) Cell Line Generation – v1
Authors: Bentley-DeSousa Amanda , Ferguson Shawn
Published: December 2024

This protocol details steps taken to create KO cell lines in RAW 264.7 cells.
Experimental validation for transcoding scheme named R+ in DNA data storage v1
Authors: Liu Deruilin , Xu Demin , Shi Liuxin , Zhang Jianyuan , Bi Kewei , Luo Bei , Liu Chen , Li Yuxiang , Fan Guangyi , Wang Wen , Ping Zhi
Published: December 2024

We have developed a universal transcoding scheme named R+ for expanded molecular alphabet and demonstrating the in vitro experimental validation of DNA data storage containing 5-methylcytosine (5mC) from data writing to data reading. The experimental validation mainly included assembly of 5mC fragments and nanopore sequencing. For more information see the accompanying paper and the following protocol.
scRNA-seq protocol v1
Authors: Luo Renhe , Beer Michael , Huangfu Danwei
Published: December 2024

scRNA-seq protocol for ESC DE differentiation time course
SPATIOTEMPORAL METAPHORS – BOLESŁAW LEŚMIAN’S “ZWIEWNOŚĆ” IN TRANSLATIONS
Authors: Ukrainets Magdalena
Published: December 2024

The article “Spatiotemporal Metaphors – Bolesław Leśmian’s “Zwiewność” in Translations” focuses on analyzing the concept of “zwiewność” (airiness) in Bolesław Leśmian’s work, with particular emphasis on the poem “Zwiewność”. The author presents how Leśmian transforms the traditional understanding of “zwiewność” — as a quality of light, ephemeral, and transient matter — into an existential context where it becomes a term for the way human existence and the world are perceived over time. The article highlights how Leśmian, using juxtaposition and micro-images, creates a narrative that ultimately forms a general principle of being in the world. The author also examines the challenges associated with translating this concept into other languages, noting that different translations introduce varying connotations, potentially altering the metaphors related to existence. She provides a detailed analysis of the differences in interpreting “zwiewność” in translations into Czech, Slovak, and Ukrainian, pointing out the variations in meaning and the diversity of translation strategies used. The article also includes a formal analysis of Leśmian’s text, emphasizing the importance of preserving the original structure in translation to convey the metaphors’ meaning accurately. Ukrainets observes that each translator, depending on the strategies employed, introduces new meanings into the text, which often leads to a change in the interpretation of key metaphors, such as “the airy density of the body”. The author of the article notes how metaphor transformations and translation choices affect the reception and understanding of Leśmian’s work in other languages.
REVIEW OF BEGINNER-LEVEL GUITARS FROM A TECHNICAL, MUSICAL, AND STRUCTURAL POINT OF VIEW: THE CASE OF TURKEY
Authors: Gayretli Şerif
Published: December 2024

The demand caused by the decreasing number of tree species used in guitar production every day is being tried to be met with artificial materials in the 21st century. Guitars are produced mainly in countries where cheap labor and materials are sold at affordable prices, attracting much attention in Turkey and the rest of the world. This type of guitar, sold in some chain markets in Turkey several times a year, attracts the attention of those who mainly want to get a guitar education at the beginner level. This research aims to determine the technical, musical, and structural competence level of imported guitars used at the beginner level in Turkey. In the research conducted in the case study pattern, we studied five people who are experts in their field. "Guitar Technical and Musical Evaluation Form" and "Guitar Structural Evaluation Form" were used as data collection tools by expert opinions. The obtained data were analyzed with the appropriate computer program. The content analysis of the answers given to the open-ended questions was carried out. As a result of the research, it has been found that the guitars in question are regular in appearance, but they are technically and musically found to be incompetent. However, it has been found that the guitars are structurally proportional, but the wood quality needs to be improved. All experts have stated that these instruments have an intonation problem and are unsuitable for guitar education. As a result of the research, it is proposed to officially determine the standards of guitars and allow their import within the framework of these standards.
Efeito do cobre em Psidium guajava com adição de bagaço de laranja no solo
Authors: Lazzaretti Giuvana , Hildebrand Soriani Hilda , Da Silva Laura Regina , Pansanato Aguiar Cruz Felippe , Wastowski Arci Dirceu , Mantovani Nilton César
Published: December 2024

A citricultura desenvolveu-se muito, trazendo consigo o desenvolvimento das indústrias processadoras da laranja, no entanto, a geração de resíduos nesta atividade industrial é elevada. Objetivou-se avaliar a tolerância da espécie Psidium guajava em solo contaminado com cobre e adição de bagaço de laranja. O experimento foi conduzido de 01/2021 até 09/2021, e os tratamentos consistiram de 3 proporções de bagaço incorporado ao solo, e contaminados com diferentes concentrações de cobre, e transplantado as mudas. Determinou-se a massa seca, teor de cobre no composto e tecidos, cobre acumulado nos tecidos e índice de translocação. O delineamento experimental foi inteiramente casualizado em esquema fatorial. A contaminação com cobre promoveu acréscimos nos teores de cobre total do composto, e a proporção de 50% de resíduo apresentou valores superiores. O cobre interferiu no desenvolvimento das mudas, reduzindo a massa seca, e elevou os teores de cobre acumulado nas plantas. As proporções 25 e 50% de resíduo promoveram reduções nos teores de cobre acumulado nas raízes e total, e na parte aérea, a proporção de 25% de resíduo promoveu aumento nos teores de cobre acumulado em comparação às demais. O índice de translocação reduziu com o aumento do cobre no composto e apresentou menores valores na proporção 0% de resíduo. A adição de 50% de resíduo e as concentrações de 200 e 400 mg Cu.Kg-1 promoveram reduções na qualidade das mudas, verificada através de decréscimos de massa seca, além disso, através do índice de translocação, a Psidium guajava não mostrou-se uma planta viável para fitorremediação.
Qualidade de sementes de aveia branca (Avena sativa L.) em resposta a doses de fungicidas
Authors: Renz Scheer Michele , Carbonera Roberto , Gonzalez da Silva José Antônio , Udich Bester Adriano , Kristoschik Jozier , Cossetim da Silva Franciele
Published: December 2024

A aveia branca é uma espécie importante por se inserir em diversos sistemas de produção e pela necessidade de desenvolvimento de práticas sustentáveis, com a minimização dos efeitos adversos decorrentes do uso de pesticidas e fertilizantes sintéticos sobre a saúde humana e ambiental. Diante disso, o objetivo do presente trabalho foi avaliar a qualidade fisiológica, sanitária e resíduos de pesticidas em sementes de aveia manejadas sob diferentes aplicações de fungicidas. O delineamento experimental utilizado foi de blocos casualizados, seguindo esquema fatorial 3 x 6, para três cultivares de aveia branca e seis condições de aplicação de fungicida, respectivamente, com quatro repetições. Em laboratório, foram realizadas as análises de germinação, vigor, patologia e resíduos de pesticidas. Realizou-se análise de variância, teste de comparação de médias de Scott & Knott e regressão. Frente aos resultados obtidos, a análise de qualidade fisiológica de sementes indicaram níveis elevados de germinação e vigor, mesmo sob condições de elevada ocorrência de patógenos. Resíduos de fungicidas foram constatados a partir da segunda aplicação e acima do limite máximo com cinco aplicações.
Agroecossistemas multifuncionais sustentáveis para produção de manga no semiárido: qualidade química e física do solo
Authors: Severo Rafael Maria Raphaela , Monteiro Salviano Alessandra , Giongo Vanderlise , Bezerra de Melo Stefeson , Olszevski Nelci , Rodrigues Oliveira Vitória
Published: December 2024

O objetivo do trabalho foi avaliar se o manejo do solo e o tipo de mistura de plantas de cobertura, componentes de agroecossistemas multifuncionais, alteram a qualidade do solo em um pomar de mangueira cv. ‘Kent’ irrigado em ambiente semiárido, bem como identificar propriedades químicas e físicas com potencial para uso como indicadores de qualidade do solo. Os dados utilizados fazem parte de um estudo de longa duração que avalia duas práticas de manejo do solo associadas a misturas de plantas de cobertura (coquetéis vegetais). O experimento foi instalado em 2007 e, para esse trabalho foram utilizados o 2o (2008) e o 9o ciclos (2017) de cultivo. Os dados foram submetidos às análises de componentes principais (ACP) e de agrupamento (AA). A prática de não revolver (SR) proporciona melhoria na qualidade do solo na camada mais superficial do solo (0 – 0,05 m) independente da composição das plantas de cobertura utilizada. Além disso, proporciona maior acúmulo de C no solo da entrelinha do pomar de mangueira. As variáveis identificadas como potenciais indicadores para monitoramento da qualidade do solo para os diferentes agroecossistemas multifuncionais frutícolas avaliados, em condições semiáridas irrigadas, são: acidez potencial, soma de bases, CTC, saturação por bases, teores de C, P, Ca, Mg, K e Na, além da microporosidade e porosidade total.
Metais potencialmente tóxicos em solos cultivados com cana-de-açúcar
Authors: Vieira Oliveira Rozilda , Cotrim Duete Washington Luiz
Published: December 2024

A qualidade do solo interfere na capacidade de produção dos sistemas agrícolas. O uso intensivo de insumos e a ausência de rotação de cultura contribuem para a degradação química do solo. Na região do Recôncavo da Bahia, o cultivo de cana-de-açúcar, iniciado desde o período colonial, ainda permanece em alguns municípios como monocultura. Este estudo foi desenvolvido nos municípios de Amélia Rodrigues e São Sebastião do Passé, Bahia, com o objetivo de avaliar os teores pseudototais de metais em solos cultivados com cana-de-açúcar e adubados com torta de filtro, vinhaça, N, P e K. As amostras de solos foram coletadas em glebas de cana-de-açúcar nas profundidades de 0 a 20 cm e 20 a 40 cm, contemplando quatro classes de solos: Vertissolo Háplico (VCo), Vertissolo Hidromórfico (VGz), Luvissolo Crômico (TCo) e Argissolo Amarelo (PAdx). Para avaliação dos metais Cd, Co, Cr, Cu, Mn, Ni, Pb, Zn e Fe foi utilizado o extrator água régia, tendo as determinações analíticas sido feitas por espectrometria de absorção atômica. Nos Vertissolos, os teores de Cd, Co e Mn foram superiores aos valores máximos recomendados para uso agrícola do solo. No Luvissolo, apenas o Cd registrou teores acima do valor de prevenção e no Argissolo as concentrações dos metais ficaram abaixo dos valores de prevenção. Os resultados indicam a necessidade de um programa de monitoramento da qualidade do solo com ações preventivas, visando a assegurar sua qualidade de forma compatível com o uso previsto.
Potential of cassava by-product and lychee pulp in the production of reduced-fat ice cream
Authors: Cervejeira Bolanho Barros Beatriz , Moreira Terhaag Marcela , Dionísio Gomes Roberto , Lilian Gallani Silva Dayane , Jaime dos Santos Previatti Caio
Published: December 2024

The aim of this study was to develop and characterize reduced-fat ice cream formulations with the addition of dehydrated cassava bagasse (CB) and lychee pulp (LP). The ice cream formulations were prepared with different proportions of palm fat (0 to 5%), CB (0 to 5%) and LP (1 to 8%) and were evaluated in terms of chemical and microbiological composition, and in relation to the technological, antioxidant and sensory properties. The use of higher levels of CB (5%) and LP (8%) allowed obtaining a higher fiber content and lower fat content, and all formulations were safe for consumption, and it was noted adequate values for overrun, melting rate and color. The ice creams showed adequate sensory acceptance, with emphasis on the formulations containing different amounts of CB addition (2 to 5%) and 2% LP. The higher LP content the higher total phenolic content and the antioxidant capacity were found in ice cream formulations. These results showed the viability of applying CB and LP in ice cream formulations, promoting the sustainability by using a natural source of antioxidants and a fiber-rich agro-industrial by-product.
The algorithms of efficient control of the biodynamic lighting system of the ‘Dom.RF’ pavilion facility of the ‘Russia’ exhibition
Authors: Degtyareva Svetlana V. , Sidorov Boris N. , Lazarev Dmitry B.
Published: December 2024

BACKGROUND: Aspects related to daily human activities, features of indoor activities, such as lighting settings, integration with building systems and changing locations during the day in various places, are largely ignored in lighting control systems based on generally applicable values and regulatory standards. The modern intensive rhythm of life, which implies the widespread use of new, constantly improving technologies, frequent changes in the environment — all this creates the need for a modern approach to the study and application of an intelligent lighting system.
AIM: Development and research of operability and functioning quality of the electro-technical facilities, systems and their components in various modes, under various external influences.
METHODS: Design of lighting systems in the Autodesk AutoCAD software, calculation of quantity and activation areas, as well as implementation of biodynamic lighting using the AWADA application.
RESULTS: In this paper, the study object is the ‘Dom.RF’ pavilion facility built in 2023 for the ‘Russia’ exhibition on the territory of the Exhibition of Achievements of National Economy (VDNKh). The pavilion was planned to show the image of a new market for individual housing construction, ensuring comfortable living in a harmonious environment [2]. This paper contains a technical and economic comparison of the implemented lighting systems and the use of biodynamic lighting. The practical value of the study lies in the operability testing, development and design of the biodynamic lighting designed for a public building used as an exhibition pavilion.
CONCLUSION: A complete package of measures for replacement with the development of an effective, ecological and safe implementation of biodynamic lighting for the technical facility of the pavilion, including the creation and operation of components, has been developed.
Clinical experience in the study of thrombodynamics in pregnant women at risk of developing preeclampsia and during its manifestation
Authors: Mozgovaya Elena V. , Peretyatko Ilya S. , Kornyushina Ekaterina A. , Androsova Natalia E. , Oparina Tatiana I. , Antonova Maria I. , Georgieva Irina I. , Gerzhan Ksenia А. , Kogan Igor Yu.
Published: December 2024

BACKGROUND: Need for early diagnosis of intravascular activation of coagulation in obstetric patients at risk of preeclampsia.
AIM: The aim of this study was to assess the diagnostic value of the thrombodynamics test in patients at risk of developing preeclampsia and during its manifestation.
MATERIALS AND METHODS: Dynamic thrombophotometry was performed in 56 pregnant women using a domestic “T2 Thrombodynamics Recorder” device (GemaKor Ltd., Russia). The first study group consisted of patients in the second to early third trimester of pregnancy (n = 45), who, due to hypercoagulability, according to the conventional coagulogram, received low-molecular-weight heparin therapy (calcium nadroparin 0.3 ml or enoxaparin sodium 0.4 ml subcutaneously). The thrombodynamics test was performed to evaluate the efficacy of anticoagulant therapy. The second study group involved patients with moderate (n = 11) and severe (n = 8) preeclampsia.
RESULTS: The test was effective in monitoring the state of the hemostasis system in pregnant women receiving anticoagulant therapy — despite normal coagulogram test parameters, the increased rate of fibrin clot formation and / or spontaneous clots indicated increased anticoagulant therapy in 37.8% of patients. Among patients with extragenital pathology, the incidence of spontaneous clots, which should not form during normal coagulation, was observed in all pregnant women with chronic arterial hypertension combined with obesity (χ2 = 6.11; p 0.02), which characterizes the tendency to activate intravascular coagulation in such patients. Subsequently, moderate PE developed in 15.6% of pregnant women with predisposing extragenital pathology, there being no cases of severe preeclampsia. A comparison of thrombodynamics test parameters in patients with moderate and severe preeclampsia showed that in severe preeclampsia, there is a tendency to a higher rate of fibrin clot formation and a higher frequency of spontaneous clot formation (χ2 = 12.7; p 0.01). The test demonstrated high sensitivity (81.8%) and specificity (97.4%) of signaling the presence of severe preeclampsia.
CONCLUSIONS: Demonstration of a clinical case of HELLP syndrome in a pregnant woman showed that confirmation of consumption thrombocytopenia due to activation of intravascular coagulation using the thrombodynamics test, in contrast to the global thromboelastography test and the conventional coagulogram test, allowed timely initiation of anticoagulant therapy and avoided the development of irreversible secondary microangiopathy.
Seasonal changes in absolute and relative organ weight in 3-month male Wistar rats
Authors: Areshidze David A. , Kondashevskaya Marina V. , Kozlova Maria A. , Anurkina Anna I. , Kasabov Kirill A.
Published: December 2024

BACKGROUND: To correctly interpret preclinical studies and basic research, it is necessary to accurately assess the biometric parameters of normal status and their limits. Biometric parameters of animals of genetically pure lines are known to vary according to factors such as sex and age, but little attention is paid to a seasonal factor. Therefore, it seems relevant for physiology and pathophysiology to develop reference values of normal status and their limits for working with genetically pure animals during certain periods of ontogenesis and different seasons.
AIM: The aim of the study was to determine the absolute body and organ weights and the relative organ weights of adult male Wistar rats at three months of age during different seasons of the year.
MATERIALS AND METHODS: The experiment involved 160 male Wistar rats. The animals were divided into four groups so that the rats reached the age of three months in one of the seasons: spring, summer, fall, or winter. In each group, absolute and relative body and organ weights were measured for the brain, spleen, thymus, liver, kidneys, adrenal glands, heart, lungs, and testes.
RESULTS: The most significant seasonal differences were found in the absolute weights of all organs, while the relative weight coefficients of the organs differed in only 50% of the cases. Synchronous seasonal differences in kidney and adrenal gland weights were observed. The heart was found to have the most significant seasonal variability in both weight parameters studied.
CONCLUSIONS: The absolute organ weight is one of the most sensitive parameters in determining reference values, but changes in the relative weight coefficient should also be considered. This study established reference ranges for the weight of major organs in a population of healthy, genetically homogeneous Wistar rats at three months of age and showed seasonal variations in these reference ranges. Further research is needed to understand changes in organ weights combined with pathological data.