bluuebunny/crossref_metadata_2025 huggingface dataset:
Muscle Oxygen Extraction during Vascular Occlusion Test in Physically Very Active versus Inactive Healthy Men: A Comparative Study
Authors: Tuesta Marcelo , Yáñez-Sepúlveda Rodrigo , Monsalves-Álvarez Matías , Vásquez-Bonilla Aldo , Olivares-Arancibia Jorge , Rojas-Valverde Daniel , Alvear-Órdenes Ildefonso
Published: March 2024

An increase in the delivery and use of oxygen to the musculature in physically active subjects are determinants of improving health-related aerobic capacity. Additional health benefits, such as an increase in the muscle mass and a decrease in fat mass, principally in the legs, could be achieved with weekly global physical activity levels of more than 300 min. The objective was to compare the muscle vascular and metabolic profiles of physically very active and inactive subjects. Twenty healthy men participated in the study; ten were assigned to the physically very active group (25.5 ± 4.2 years; 72.7 ± 8.1 kg; 173.7 ± 7.6 cm) and ten to the physically inactive group (30.0 ± 7.4 years; 74.9 ± 11.8 kg; 173.0 ± 6.4 cm). The level of physical activity was determined by the Global Physical Activity Questionnaire (GPAQ). A resting vascular occlusion test (5 min of an ischemic phase and 3 min of a reperfusion phase) was used, whereas a near-field infrared spectroscopy (NIRS) device was used to evaluate the muscle oxygenation in the right vastus lateralis of the quadriceps muscle. The area under the curve of the deoxyhemoglobin (HHb) during the ischemic phase and above the curve of the tissue saturation index (TSI) during the reperfusion phase were obtained to determine muscle metabolic and vascular responses, respectively. Physically very active group showed a higher absolute HHb (3331.9 ± 995.7 vs. 6182.7 ± 1632.5 mmol/s) and lower TSI (7615.0 ± 1111.9 vs. 5420.0 ± 781.4 %/s) and relative to body weight (46.3 ± 14.6 vs. 84.4 ± 27.1 mmol/s/kg and 106.0 ± 20.6 vs. 73.6 ± 13.8 %/s/kg, respectively), muscle mass (369.9 ± 122.2 vs. 707.5 ± 225.8 mmol/kg and 829.7 ± 163.4 vs. 611.9 ± 154.2 %/s/kg) and fat mass (1760.8 ± 522.9 vs. 2981.0 ± 1239.9 mmol/s/kg and 4160.0 ± 1257.3 vs. ±2638.4 ± 994.3 %/s/kg, respectively) than physically inactive subjects. A negative correlation was observed between HHb levels and TSI (r = −0.6; p < 0.05). Physically very active men (>300 min/week) present better muscle oxidative metabolism and perfusion and perform significantly more physical activity than physically inactive subjects. Extra benefits for vascular health and muscle oxidative metabolism are achieved when a subject becomes physically very active, as recommended by the World Health Organization. In addition, a higher level of physical activity determined by GPAQ is related to better vascular function and oxidative metabolism of the main locomotor musculature, i.e., the quadriceps.
Research on Dynamic Quaternion Ship Domain Model in Open Water Based on AIS Data and Navigator State
Authors: Liu Dongqin , Zheng Zhongyi , Liu Zihao
Published: March 2024

During the process of establishing the analytical quaternion ship domain model, the impact of ship traffic conditions and navigator state was not taken into consideration. However, the significance of these factors in the ship domain cannot be ignored. To create a more realistic representation of changes in the ship domain in real navigation environments, this study further considers the influence of ship encounter course, waterway traffic intensity, relative ship velocity, and the navigator state based on the quaternion ship domain model. As a result, a new dynamic quaternion ship domain model is proposed. To assess the changes in the size and shape of the ship domain under various navigation environments, ship domain scaling and shape transformation functions are introduced. Specifically, this study focuses on analyzing the ship traffic near the Lao Tie Shan Waterway, simulating the size and shape changes of the ship domain during the navigation process in this area. The findings indicate that the size of the ship domain dynamically adjusts to the traffic conditions. Additionally, when the navigator state is excellent, the ship domain takes on an irregular diamond shape with the smallest area, whereas when the navigator state is poor, the shape approximates a rectangle with the largest area. Furthermore, the dynamic quaternion ship domain model proposed in this study is compared to the ship domain models put forth by Goodwin, Davis, and co-authors. The results demonstrate that the dynamic quaternion ship domain model is more compatible and suitable for open waters compared to the static quaternion ship domain model.
The Carbonate System of Penzhina Bay and the Shelikhov Gulf in the Sea of Okhotsk during Extreme Tides in Summer
Authors: Semkin Pavel , Baigubekov Kirill , Barabanshchikov Yuri , Gorin Sergey , Koltunov Alexey , Sagalaev Sergey , Ulanova Olga , Tishchenko Petr , Shvetsova Maria , Shkirnikova Elena , Tishchenko Pavel , Zhang Jing
Published: March 2024

Understanding the factors that control carbonate systems is an important goal due to the complex interactions between the hydrophysical and chemical–biological conditions in coastal basins. The results of this paper present the state of the carbonate system in Penzhina Bay and its adjacent waters—the Shelikhov Gulf—in July 2023, during spring tides with 13 m height. The area we studied included the length of the largest river in the region, the Penzhina River, from the peak of its summer flood to its boundary with the Shelikhov Gulf (the Sea of Okhotsk). This unique dynamic basin, with a length of about 800 km, was studied over 17 days. During this period, the entire water column of Penzhina Bay, down to a depth of about 60 m, and the surface water layer of the Shelikhov Gulf were undersaturated in terms of CO2, with low levels relative to those of the atmosphere. To explain this observation, the dissolved oxygen, nutrients in mineral and organic forms, humic substances, chlorophyll a, and photic zone thickness are presented for the entire basin under study, together with its hydrological data. The results of daily observations of the carbonate system at fixed anchorage stations characterize two contrasting regions of Penzhina Bay: one that was more exposed to continental runoff, which had salinity levels in the range of 8.0–21.3 psu during one tidal cycle; the second had smaller variations in salinity in the range of 31.6–32.9 psu during one tidal cycle. This study emphasizes the importance of biological processes and continental runoff on the variability of the carbonate system parameters and CO2 fluxes at a water/atmosphere boundary with extreme tidal conditions in this ecosystem that is barely affected by human activities.
Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection
Authors: Sánchez-Torres Paloma , González-Candelas Luis , Ballester Ana Rosa
Published: March 2024

Green mold caused by Penicillium digitatum (Pers.:Fr.) Sacc is the most prevalent postharvest rot concerning citrus fruits. Using the subtractive suppression hybridization (SSH) technique, different P. digitatum genes have been identified that could be involved in virulence during citrus infection in the early stages, a crucial moment that determines whether the infection progresses or not. To this end, a comparison of two P. digitatum strains with high and low virulence has been carried out. We conducted a study on the gene expression profile of the most relevant genes. The results indicate the importance of transcription and regulation processes as well as enzymes involved in the degradation of the plant cell wall. The most represented expressed sequence tag (EST) was identified as PDIP_11000, associated with the FluG domain, which is putatively involved in the activation of conidiation. It is also worth noting that PDIP_02280 encodes a pectin methyl esterase, a cell wall remodeling protein with a high expression level in the most virulent fungal strains, which is notably induced during citrus infection. Furthermore, within the group with the greatest representation and showing significant induction in the early stages of infection, regulatory proteins (PDIP_68700, PDIP_76160) and a chaperone (PDIP_38040) stand out. To a lesser extent, but not less relevant, it is worth distinguishing different regulatory proteins and transcription factors, such as PDIP_00580, PDIP_49640 and PDIP_78930.
A New Genus of the Microascaceae (Ascomycota) Family from a Hypersaline Lagoon in Spain and the Delimitation of the Genus Wardomyces
Authors: Barnés-Guirado María , Stchigel Alberto Miguel , Cano-Lira José Francisco
Published: March 2024

The Saladas de Sástago-Bujaraloz is an endorheic and arheic complex of lagoons located in the Ebro Basin and protected by the Ramsar Convention on Wetlands. Due to the semi-arid climate of the region and the high salinity of their waters, these lagoons constitute an extreme environment. We surveyed the biodiversity of salt-tolerant and halophilic fungi residents of the Laguna de Pito, a lagoon belonging to this complex. Therefore, we collected several samples of water, sediments, and soil of the periphery. Throughout the study, we isolated 21 fungal species, including a strain morphologically related to the family Microascaceae. However, this strain did not morphologically match any of genera within this family. After an in-depth morphological characterization and phylogenetic analysis using a concatenated sequence dataset of four phylogenetically informative molecular markers (the internal transcribed spacer region (ITS) of the nuclear ribosomal DNA (nrDNA); the D1-D2 domains of the 28S gene of the nuclear ribosomal RNA (LSU); and a fragment of the translation elongation factor 1-alpha (EF-1α) and the β-tubulin (tub2) genes), we established the new genus Dactyliodendromyces, with Dactyliodendromyces holomorphus as its species. Additionally, as a result of our taxonomic study, we reclassified the paraphyletic genus Wardomyces into three different genera: Wardomyces sensu stricto, Parawardomyces gen. nov., and Pseudowardomyces gen. nov., with Parawardomyces ovalis (formerly Wardomyces ovalis) and Pseudowardomyces humicola (formerly Wardomyces humicola) as the type species of their respective genera. Furthermore, we propose new combinations, including Parawardomyces giganteus (formerly Wardomyces giganteus) and Pseudowardomyces pulvinatus (formerly Wardomyces pulvinatus).
The Role of Artificial Intelligence in Endocrine Management: Assessing ChatGPT’s Responses to Prolactinoma Queries
Authors: Şenoymak Mustafa Can , Erbatur Nuriye Hale , Şenoymak İrem , Fırat Sevde Nur
Published: March 2024

This research investigates the utility of Chat Generative Pre-trained Transformer (ChatGPT) in addressing patient inquiries related to hyperprolactinemia and prolactinoma. A set of 46 commonly asked questions from patients with prolactinoma were presented to ChatGPT and responses were evaluated for accuracy with a 6-point Likert scale (1: completely inaccurate to 6: completely accurate) and adequacy with a 5-point Likert scale (1: completely inadequate to 5: completely adequate). Two independent endocrinologists assessed the responses, based on international guidelines. Questions were categorized into groups including general information, diagnostic process, treatment process, follow-up, and pregnancy period. The median accuracy score was 6.0 (IQR, 5.4–6.0), and the adequacy score was 4.5 (IQR, 3.5–5.0). The lowest accuracy and adequacy score assigned by both evaluators was two. Significant agreement was observed between the evaluators, demonstrated by a weighted κ of 0.68 (p = 0.08) for accuracy and a κ of 0.66 (p = 0.04) for adequacy. The Kruskal–Wallis tests revealed statistically significant differences among the groups for accuracy (p = 0.005) and adequacy (p = 0.023). The pregnancy period group had the lowest accuracy score and both pregnancy period and follow-up groups had the lowest adequacy score. In conclusion, ChatGPT demonstrated commendable responses in addressing prolactinoma queries; however, certain limitations were observed, particularly in providing accurate information related to the pregnancy period, emphasizing the need for refining its capabilities in medical contexts.
A New Proxy for Estimating the Roughness of Volatility
Authors: Zhao Qi , Chronopoulou Alexandra
Published: March 2024

In this paper, we propose a new proxy for the unobserved volatility process that will allow us to better understand and hence model a rough or persistent volatility. Starting with a stochastic volatility model with minimal assumptions on the volatility process, we calibrate the model to options’ data and their sensitivities to obtain an implied volatility process. Starting with this new proxy, we then study the roughness/persistence of the volatility using S&P 500 European put option daily data. We then estimate the Hurst index, i.e., roughness/smoothness parameter, of the volatility with various techniques to find that the volatility does exhibit a rough behavior, even in a low-frequency framework.
Theoretical Foundation for Pricing Climate-Related Loss and Damage in Infrastructure Financing
Authors: Assab Abderrahim
Published: March 2024

This paper presents a novel theoretical framework for incorporating climate risks and adaptation investments into infrastructure debt pricing. Utilizing the Capital Asset Pricing Model (CAPM), the framework extends the conventional modeling of infrastructure project revenues and costs to include climate risk considerations. It proposes three climate-informed revenue and cost formulations: adjustmentment of mean and standard deviation, incorporation of extreme climate events via Pareto and Poisson distributions, and a climate-informed cost model that includes adaptation investment. The paper demonstrates the application of this model in pricing a loan for a Light Rail Transit project in Costa Rica, introducing the concepts of “flood risk premium” and “adaptation curves”. This study not only offers a novel lens through which to view infrastructure investment under climate uncertainty but also sets the stage for transformative policy and practice in financial risk assessment, encouraging a shift towards more sustainable and resilient infrastructure development.
Did Urban Resilience Improve during 2005–2021? Evidence from 31 Chinese Provinces
Authors: Yang Tingting , Wang Lin
Published: March 2024

In the context of climate change, various natural disasters and extreme weather events are occurring with increasing frequency. In addition, large-scale urbanization in China poses serious challenges to disaster resilience. The convergence of climate change and large-scale urbanization has made the enhancement of urban resilience (UR) an important guideline for current urban development. This study analyzes the UR of 31 provinces in China during 2005–2021 through the entropy method. A UR evaluation index system is constructed from the perspective of population resilience, social resilience, economic resilience, safeguarding facility resilience, and ecological resilience. The results demonstrate the following: (1) The overall performance of UR in China is relatively low, with an average value of 0.2390. (2) Chinese provinces significantly differ in UR levels, with Beijing, Shanghai, Tianjin, Zhejiang, Jiangsu, and Fujian being the top performers and Guangxi, Yunnan, Xinjiang, Gansu, and Tibet being the bottom. (3) From 2005 to 2021, the average UR value of the 31 Chinese provinces significantly improved. (4) Generally, the eastern, middle, and western regions exhibit relatively high, medium, and low average UR values, respectively. These research findings provide valuable references for Chinese policymakers to adopt measures for promoting UR enhancement and urban safety.
Rapid Detection of Tannin Content in Wine Grapes Using Hyperspectral Technology
Authors: Zhang Peng , Wu Qiang , Wang Yanhan , Huang Yun , Xie Min , Fan Li
Published: March 2024

Wine grape quality is influenced by the variety and growing environment, and the quality of the grapes has a significant impact on the quality of the wine. Tannins are a crucial indicator of wine grape quality, and, therefore, rapid and non-destructive methods for detecting tannin content are necessary. This study collected spectral data of Pinot Noir and Chardonnay using a geophysical spectrometer, with a focus on the 500–1800 nm spectrum. The spectra were preprocessed using Savitzky–Golay (SG), first-order differential (1D), standard normal transform (SNV), and their respective combinations. Characteristic bands were extracted through correlation analysis (PCC). Models such as partial least squares (PLS), support vector machine (SVM), random forest (RF), and one-dimensional neural network (1DCNN) were used to model tannin content. The study found that preprocessing the raw spectra improved the models’ predictive capacity. The SVM–RF model was the most effective in predicting grape tannin content, with a test set R2 of 0.78, an RMSE of 0.31, and an RE of 10.71%. These results provide a theoretical basis for non-destructive testing of wine grape tannin content.
Low Left-Ventricular Ejection Fraction as a Predictor of Intraprocedural Cardiopulmonary Resuscitation in Patients Undergoing Transcatheter Aortic Valve Implantation
Authors: Gerfer Stephen , Großmann Clara , Gablac Hannah , Elderia Ahmed , Wienemann Hendrik , Krasivskyi Ihor , Mader Navid , Lee Samuel , Mauri Victor , Djordjevic Ilija , Adam Matti , Kuhn Elmar , Baldus Stephan , Eghbalzadeh Kaveh , Wahlers Thorsten
Published: March 2024

Transcatheter aortic valve replacement (TAVR) has become an established alternative to surgical aortic valve replacement (AVR) for patients with moderate-to-high perioperative risk. Periprocedural TAVR complications decrease with growing expertise of implanters. Nevertheless, TAVR can still be accompanied by life-threatening adverse events such as intraprocedural cardiopulmonary resuscitation (CPR). This study analyzed the role of a reduced left-ventricular ejection fraction (LVEF) in intraprocedural complications during TAVR. Perioperative and postoperative outcomes from patients undergoing TAVR in a high-volume center (600 cases per year) were analyzed retrospectively with regard to their left-ventricular ejection fraction. Patients with a reduced left-ventricular ejection fraction (EF ≤ 40%) faced a significantly higher risk of perioperative adverse events. Within this cohort, patients were significantly more often in need of mechanical ventilation (35% vs. 19%). These patients also underwent CPR (17% vs. 5.8%), defibrillation due to ventricular fibrillation (13% vs. 5.4%), and heart–lung circulatory support (6.1% vs. 2.5%) more often. However, these intraprocedural adverse events showed no significant impact on postoperative outcomes regarding in-hospital mortality, stroke, or in-hospital stay. A reduced preprocedural LVEF is a risk factor for intraprocedural adverse events. With respect to this finding, the identified patient cohort should be treated with more caution to prevent intraprocedural incidents.
Unraveling the Complexities of Oxidative Stress and Inflammation Biomarkers in Obstructive Sleep Apnea Syndrome: A Comprehensive Review
Authors: Lavalle Salvatore , Masiello Edoardo , Iannella Giannicola , Magliulo Giuseppe , Pace Annalisa , Lechien Jerome Rene , Calvo-Henriquez Christian , Cocuzza Salvatore , Parisi Federica Maria , Favier Valentin , Bahgat Ahmed Yassin , Cammaroto Giovanni , La Via Luigi , Gagliano Caterina , Caranti Alberto , Vicini Claudio , Maniaci Antonino
Published: March 2024

Background: Obstructive sleep apnea syndrome (OSAS), affecting approximately 1 billion adults globally, is characterized by recurrent airway obstruction during sleep, leading to oxygen desaturation, elevated carbon dioxide levels, and disrupted sleep architecture. OSAS significantly impacts quality of life and is associated with increased morbidity and mortality, particularly in the cardiovascular and cognitive domains. The cyclic pattern of intermittent hypoxia in OSAS triggers oxidative stress, contributing to cellular damage. This review explores the intricate relationship between OSAS and oxidative stress, shedding light on molecular mechanisms and potential therapeutic interventions. Methods: A comprehensive review spanning from 2000 to 2023 was conducted using the PubMed, Cochrane, and EMBASE databases. Inclusion criteria encompassed English articles focusing on adults or animals and reporting values for oxidative stress and inflammation biomarkers. Results: The review delineates the imbalance between pro-inflammatory and anti-inflammatory factors in OSAS, leading to heightened oxidative stress. Reactive oxygen species biomarkers, nitric oxide, inflammatory cytokines, endothelial dysfunction, and antioxidant defense mechanisms are explored in the context of OSAS. OSAS-related complications include cardiovascular disorders, neurological impairments, metabolic dysfunction, and a potential link to cancer. This review emphasizes the potential of antioxidant therapy as a complementary treatment strategy. Conclusions: Understanding the molecular intricacies of oxidative stress in OSAS is crucial for developing targeted therapeutic interventions. The comprehensive analysis of biomarkers provides insights into the complex interplay between OSAS and systemic complications, offering avenues for future research and therapeutic advancements in this multifaceted sleep disorder.
Method for the Statistical Analysis of the Signals Generated by an Acquisition Card for Pulse Measurement
Authors: Pantoja-Pacheco Yaquelin Verenice , Yáñez-Mendiola Javier
Published: March 2024

This article shows a method for the statistical analysis of signals. Firstly, this method was applied to analyze the processing of signs generated by an acquisition card for pulse measurement using the synchronous demodulation method. The application of the method allowed the study of each signal consisting of a descriptive statistical analysis, followed by the analysis of the trend and dynamics of the movement using the augmented Dickey–Fuller test and Hurst exponent, respectively. Secondarily, the method presented here supported the comparison between the pulse signals obtained by synchronous demodulation and plethysmography methods. In addition, the residuals from the pulse comparison of both methods were analyzed. To quantify the differences between the signals, these were compared using the mean-squared error, the root-mean-square error, the mean absolute error, the mean error, the mean absolute percentage error, and the mean percentage error. After this research, it was possible to analyze the signals knowing characteristics such as the following: the presence of normal, exponential, lognormal, and uniform distributions, stationary trend, and dynamic movement anti-persistent. The novelty that this article proposes is the use of concepts traditionally used in the study of time series and models of demand administration, now focused on supporting improvements over the different stages of design and conceptualization of signal processing devices.
On Soft ωδ-Open Sets and Some Decomposition Theorems
Authors: Abuzaid Dina , Al-Ghour Samer , Naghi Monia
Published: March 2024

In this paper, we present a novel family of soft sets named “soft ωδ-open sets”. We find that this class constitutes a soft topology that lies strictly between the soft topologies of soft δ-open sets and soft ω0-open sets. Also, we introduce certain sufficient conditions for the equivalence between this new soft topology and several existing soft topologies. Moreover, we verify several relationships that contain soft covering properties, such as soft compactness and soft Lindelofness, which are related to this new soft topology. Furthermore, in terms of the soft interior operator in certain soft topologies, we define four classes of soft sets. Via them, we obtain new decomposition theorems for soft δ-openness and soft θ-openness, and we characterize the soft topological spaces that have the soft “semi-regularization property”. In addition, via soft ωδ-open sets, we introduce and investigate a new class of soft functions named “soft ωδ-continuous functions”. Finally, we look into the connections between the newly proposed soft concepts and their counterparts in classical topological spaces.
Mathematical Modeling of Multi-Phenomena Anisotropic Systems: Ejection of Primary Aerosols during the Fast Pyrolysis of Biomass
Authors: Sánchez Mario A. , Maya Juan C. , Chejne Farid , Pecha Brennan , Quinchía-Figueroa Adriana M.
Published: March 2024

This study introduces a novel particle model for biomass fast pyrolysis, incorporating an anisotropic cylindrical particle to address mass and energy transport coupled with aerosol ejection, which previous models have overlooked. The main contribution lies in developing a model that considers aerosol generation in anisotropic cylindrical particles for the first time, addressing bubbling dynamics and bursting within the liquid phase. The population balance equation describes bubble dynamics and aerosol formation, capturing phenomena like nucleation, growth, coalescence, and bursting. The model employs the method of moments with bubble volume as an internal variable, substantially reducing computational costs by eliminating dependence on this variable. Results highlight the significant impact of anisotropy and particle size on aerosol ejection: smaller, less elongated particles experience faster heating, quicker conversion, and the increased accumulation of the liquid intermediate phase. Specifically, 1 mm diameter particles yield higher concentrations of metaplast and bio-oil aerosols, exceeding 15%, compared to concentrations below 11% for 3 mm particles. This model provides insights into aerosol structure (volume, surface area), aiding in understanding aerosol reactivity at the reactor scale.
State of the Art in 3D Culture Models Applied to Thyroid Cancer
Authors: Prete Alessandro , Matrone Antonio , Plebani Roberto
Published: March 2024

Thyroid cancer (TC) is the prevalent endocrine tumor with a rising incidence, particularly in higher-income countries, leading to an increased interest in its management and treatment. While overall, survival rates for TC are usually favorable, advanced cases, especially with metastasis and specific histotypes, pose challenges with poorer outcomes, advocating the need of systemic treatments. Targeted therapies have shown efficacy in both preclinical models and clinical trials but face issues of resistance, since they usually induce partial and transient response. These resistance phenomena are currently only partially addressed by traditional preclinical models. This review explores the limitations of traditional preclinical models and emphasizes the potential of three-dimensional (3D) models, such as transwell assays, spheroids, organoids, and organ-on-chip technology in providing a more comprehensive understanding of TC pathogenesis and treatment responses. We reviewed their use in the TC field, highlighting how they can produce new interesting insights. Finally, the advent of organ-on-chip technology is currently revolutionizing preclinical research, offering dynamic, multi-cellular systems that replicate the complexity of human organs and cancer–host interactions.
Risk Factors of Thrombophilia-Related Mutations for Early and Late Pregnancy Loss
Authors: Borsi Ema , Potre Ovidiu , Ionita Ioana , Samfireag Miruna , Secosan Cristina , Potre Cristina
Published: March 2024

Background and Objectives: This retrospective cohort study investigates the role of genetic thrombophilia in pregnant women experiencing early pregnancy loss compared to those with late pregnancy loss. Materials and Methods: Participants were categorized into early and late pregnancy loss groups based on gestational age. A total of 156 patients were included, out of which 103 had early-trimester pregnancy losses and 96 had multiple miscarriages. Results: The study revealed a synergistic effect of Factor V Leiden (FVL G1691A) and Methylenetetrahydrofolate Reductase (MTHFR C677T) mutations (coefficient 3.42). Prothrombin (PT) G20210A and β-Fibrinogen 455 G>A mutations exhibited a significant interaction (coefficient 1.98). Additionally, MTHFR A1298C and Plasminogen Activator Inhibitor-1 (PAI-1 4G/5G) mutations showed a significant interaction (coefficient 1.65). FVL G1691A and Endothelial Protein C Receptor (EPCR) allele A1/A2 mutations also demonstrated a significant association (coefficient 2.10). Lastly, MTHFR C677T and Glycoprotein IIb/IIIa T1565C mutations interacted significantly (coefficient 1.77). Risk factor analysis identified several mutations associated with early pregnancy loss, including PAI-1 4G/5G homozygous (OR 3.01), FVL G1691A heterozygous (OR 1.85), and MTHFR A1298C heterozygous (OR 1.55). Both homozygous and heterozygous MTHFR C677T mutations were significant risk factors (OR 2.38; OR 2.06), as was PT G20210A homozygous mutation (OR 1.92). The PAI-1 4G/4G homozygous variant posed a risk (OR 1.36). Late pregnancy loss was associated with MTHFR A1298C homozygous mutation (OR 3.79), β-Fibrinogen 455 G>A heterozygous mutation (OR 2.20), and MTHFR A1298C heterozygous mutation (OR 2.65). Factor XIII G1002T heterozygous mutation (OR 1.18) and PAI-1 4G/5G homozygous mutation (OR 2.85) were also significant risk factors. EPCR allele A1/A2 (OR 1.60) and A2/A3 (OR 1.73) mutations were identified as significant risk factors for late pregnancy loss. Furthermore, FVL G1691A homozygous mutation, PT G20210A homozygous mutation, MTHFR C677T heterozygous mutation, MTHFR A1298C heterozygous mutation, and EPCR allele A1/A2 were identified as significant risk factors for multiple miscarriage. Conclusions: This study highlights significant interactions and risk factors related to genetic thrombophilia mutations in different types of pregnancy loss, contributing valuable insights for miscarriage management guidelines.
Effect of Coiling Temperature on Microstructure and Properties of Ferritic-Bainitic Dual-Phase Steels
Authors: Li Zhengrong , Zhou Feng , Liu Jinhai , Liu Lei , Wang Chuangwei , Zhao Zhengzhi
Published: March 2024

In this study, a 780 MPa grade ferritic-bainitic dual-phase steel with excellent matching of strength-plasticity and formability was developed using thermomechanical control processing. Optical microscopy, Scanning electron microscopy, and Electron Backscatter Diffraction techniques were used to characterize the microstructure comprehensively, and the effects of coiling temperature on the microstructure, the strength-plasticity, and hole-expansion ratio of the test steels were thoroughly investigated. The results showed that the test steel had an excellent combination of ferrite and bainite at the coiling temperature of 520 °C, 23.7 and 76.3%, respectively, with a hole expansion ratio of 58.5 ± 2.8%. The uniformity of the microstructure was the key to obtaining a high expansion ratio in ferrite-bainite dual-phase steels. The test steels formed granular bainite at low-temperature coiling, while polygonal ferrite was promoted at high-temperature coiling. The effect of coiling temperature on grain size is small. Dislocations were redistributed during high-temperature coiling, resulting in a decrease in dislocation density. The higher elongation and hole expansion rate at higher coiling temperatures were attributed to increased polygonal ferrite content, reduced grain size, and enhanced TRIP effect. When coiling at low temperatures, the agglomeration of polygonal ferrite or granular bainite tends to result in a non-uniform distribution of the soft and hard phases of the matrix. At the same time, the strong texture parallel to the rolling direction has a significant difference in plasticity in different directions, leading to non-uniform deformation, which is liable to stress concentration, causing crack nucleation and extension in the hole expanding process, thus reducing the hole expansion performance.
An Evaluation of a SARS-CoV-2 Pseudovirus Neutralization Test and A Comparison to a SARS-CoV-2 Surrogate Virus Neutralization Test in a COVID-19 Long-Term Follow-Up Cohort
Authors: Gillot Constant , Favresse Julien , David Clara , Maloteau Vincent , Dogne Jean-Michel , Douxfils Jonathan
Published: March 2024

Background: The detection of neutralizing anti-SARS-CoV-2 antibodies is important since they represent the subset of antibodies able to prevent the virus to invade human cells. The aim of this study is to evaluate the clinical performances of an in-house pseudovirus neutralization test (pVNT) versus a commercial surrogate neutralization test (sVNT). Material and Methods: A total of 114 RT-PCR positives samples from 75 COVID-19 patients were analyzed using a pVNT and an sVNT technique. Fifty-six pre-pandemic samples were also analyzed to assess the specificity of the two techniques. An analysis of the repeatability and the reproducibility of the pVNT was also performed. Results: A coefficient of variation (CV) of 10.27% for the repeatability of the pVNT was computed. For the reproducibility test, CVs ranged from 16.12% for low NAbs titer to 6.40% for high NAbs titer. Regarding the clinical sensitivity, 90 RT-PCR positive samples out of 114 were positive with the pVNT (78.94%), and 97 were positive with the sVNT (84.21%). About the clinical specificity, all 56 pre-pandemic samples were negative in both techniques. When comparing the sVNT to the pVNT, the specificity and sensibility were 66.67% (95%CI: 47.81–85.53%) and 98.88% (95%CI: 96.72–99.99%), respectively. Conclusions: The results obtained with the automated sVNT technique are consistent with those obtained with the pVNT technique developed in-house. The results of the various repeatability and reproducibility tests demonstrate the good robustness of the fully manual pVNT technique.
Dinoflagellate Proton-Pump Rhodopsin Genes in Long Island Sound: Diversity and Spatiotemporal Distribution
Authors: Zhang Huan , Nulick Kelly J. , Burris Zair , Pierce Melissa , Ma Minglei , Lin Senjie
Published: March 2024

Microbial proton-pump rhodopsin (PPR)-based phototrophy, a light-harvesting mechanism different from chlorophyll-based photosystems, may contribute significantly to solar energy entry into the marine ecosystem. PPR transforms solar energy into cellular energy that is used for various metabolic processes in the cells or flagellar movement. Although rhodopsins or their encoding genes have been documented in a wide phylogenetic range of cultured dinoflagellates, information is limited about how widespread and how spatiotemporally dynamical dinoflagellate PPR (DiPPR) are in natural marine ecosystems. In this study, we investigated DiPPR in Long Island Sound (LIS), a temperate estuary of the Atlantic Ocean between Connecticut and Long Island, New York, USA. We isolated six novel full-length dinoflagellate proton-pump rhodopsin cDNAs, expanding the DiPPR database that is crucial to PPR research. Based on these new sequences and existing sequences of DiPPR, we designed primers and conducted quantitative PCR and sequencing to determine the abundance and diversity of DiPPR genes spatially and temporally throughout a year in the water samples collected from LIS. DiPPR genes were found year-round and throughout LIS but with higher abundances in the eutrophic Western Sound and in April and July. The gene diversity data suggest that there are at least five distinct rhodopsin-harboring groups of dinoflagellates throughout the year. The abundance of DiPPR genes, measured as copy number per mL of seawater, appeared not to be influenced by water temperature or nitrogen nutrient concentration but exhibited weak negative correlations with orthophosphate concentration and salinity and a positive correlation with the abundance of DiPPR-harboring dinoflagellates. This first quantitative profiling of PPR in natural plankton reveals the prevalence and dynamics of this plastid-independent photoenergy harvesting mechanism in a temperate estuary and provides efficient DiPPR primers potentially useful for future research. Furthermore, this study shed light on the potential role of DiPPR in phosphor nutrition and dinoflagellate population, which warrants further studies.
Into the Rhythm: Evaluating Breathing Instruction Sound Experiences on the Run with Novice Female Runners
Authors: van Rheden Vincent , Harbour Eric , Finkenzeller Thomas , Meschtscherjakov Alexander
Published: March 2024

Running is a popular sport throughout the world. Breathing strategies like stable breathing and slow breathing can positively influence the runner’s physiological and psychological experiences. Sonic breathing instructions are an established, unobtrusive method used in contexts such as exercise and meditation. We argue sound to be a viable approach for administering breathing strategies whilst running. This paper describes two laboratory studies using within-subject designs that investigated the usage of sonic breathing instructions with novice female runners. The first study (N = 11) examined the effect of information richness of five different breathing instruction sounds on adherence and user experience. The second study (N = 11) explored adherence and user experience of sonically more enriched sounds, and aimed to increase the sonic experience. Results showed that all sounds were effective in stabilizing the breathing rate (study 1 and 2, respectively: mean absolute percentage error = 1.16 ± 1.05% and 1.9 ± 0.11%, percent time attached = 86.81 ± 9.71% and 86.18 ± 11.96%). Information-rich sounds were subjectively more effective compared to information-poor sounds (mean ratings: 7.55 ± 1.86 and 5.36 ± 2.42, respectively). All sounds scored low (mean < 5/10) on intention to use.
Primary Co-Occurrence of Gonadal and Extragonadal Central Nervous System (CNS) Germ Cell Tumors (GCTs): Case Report and Review of the Literature
Authors: Tengattini Francesco , Soffiati Cesare Francesco , Panciani Pier Paolo , Zeppieri Marco , Ius Tamara , Momjian Shahan , Schaller Karl , Fontanella Marco Maria , De Maria Lucio
Published: March 2024

Background: The primary co-occurrence of gonadal and extragonadal central nervous system (CNS) germ cell tumors (GCTs) has rarely been reported in the literature, and a common opinion on the underlying etiopathogenetic mechanism is lacking. Objective: We aim to investigate the pathophysiological mechanisms and genetic pathways underlying the primary co-occurrence of gonadal and CNS GCTs. Methods: We reviewed data from 29 consecutive patients with a diagnosis of CNS GCT, evaluated in our Hospital over the past 23 years, and searched for those who had at least a primary gonadal co-occurrence of GCT. A systematic review of the literature according to the PRISMA guidelines was also conducted. For a comprehensive and detailed search, PubMed, Ovid MEDLINE, and Ovid EMBASE databases have been consulted. Boolean operators and MeSH terms were used to find studies. Only articles published between 2000 and 2023 were considered. Results: Including our Institutional case report, a total of 7 patients with both testicular NGGCTs and CNS GCTs were identified (5 patients with metachronous tumors and patients with synchronous presentation). The average age at tumor diagnosis was 17 years. The cerebral histotypes reported were mixed GCTs (3 cases; 43%), pure germinomas (3 cases, 43%), and one yolk sac tumor (14%). Two out of seven cases (29%) were syndromic, one suffering from Down Syndrome and the other from Testicular Dysgenesis Syndrome. Regarding the etiology and molecular mechanism of GCT development, several gene mutations have been reported in the literature. Particularly, genetic alterations in the MAPK and/or PI3K/AKT/mTOR pathway, together with mutations of the KIT gene, have been shown to guarantee survival and transformation of mismigrated totipotent primordial germ cells, while suppressor genes allow their resistance against apoptotic death. Aberrant chromosomes have also been reported to be responsible for oncogenic transformation. It is also known that CNS and testicular GCTs share some genetic/epigenetic profiles. Conclusions: The primary co-occurrence of testicular NGGCT and extragonadal CNS GCTs is extremely rare. Genetic factors seem to play a paramount role in their etiopathogenesis. Additional research is needed to elucidate molecular mechanisms of oncogenesis in such patients.
The Role of Parental Adherence to the Mediterranean Diet and Family Time Together in Children’s Weight Status: The BeE-School Project
Authors: Duarte Ana , Martins Juliana , Silva Maria José , Augusto Cláudia , Martins Silvana Peixoto , Rosário Rafaela
Published: March 2024

The family context has been associated with children’s weight status. This study aims to investigate the association of parents’ adherence to the Mediterranean diet and family time with the weight status of children. The research is part of BeE-school, a cluster-randomized trial implemented in primary schools located in socially vulnerable contexts. A total of 735 children (380 boys and 355 girls) aged 6 to 10 participated in the study. Anthropometrics were assessed during school time, and weight status was categorized, while parents self-reported sociodemographic variables, adherence to the Mediterranean diet (MEDAS questionnaire), and family time. Children from families with higher education levels whose parents have a high adherence to the Mediterranean diet have lower odds of overweight/obesity (odds ratio (OR) 0.301, 95% CI 0.143–0.634, p = 0.002). Also, children from families with lower education levels who have more time together with their family have lower odds of overweight/obesity (OR 0.731, 95% CI 0.573–0.934, p = 0.012). The family environment, mainly family time together and adherence to the Mediterranean diet, exerts a significant influence on children’s weight status. Professionals working in children’s health should consider the family when fostering health-promoting behaviors.
Barium Chalcogenide Crystals: A Review
Authors: Kostyukova Nadezhda , Erushin Evgenii , Boyko Andrey , Shevyrdyaeva Galina , Badikov Dmitry
Published: March 2024

In recent decades, new nonlinear optical materials have been actively developed to create coherent tunable light sources in the mid-infrared (mid-IR) part of the spectrum used in a variety of scientific fields. In the present review, the main attention is focused on barium chalcogenide crystals, including their linear and nonlinear optical properties, laser-induced damage threshold (LIDT), and frequency down-conversion.
Exploring the Drought Tolerant Quantitative Trait Loci in Spring Wheat
Authors: Wang Zhong , Lai Xiangjun , Wang Chunsheng , Yang Hongmei , Liu Zihui , Fan Zheru , Li Jianfeng , Zhang Hongzhi , Liu Manshuang , Zhang Yueqiang
Published: March 2024

Drought-induced stress poses a significant challenge to wheat throughout its growth, underscoring the importance of identifying drought-stable quantitative trait loci (QTLs) for enhancing grain yield. Here, we evaluated 18 yield-related agronomic and physiological traits, along with their drought tolerance indices, in a recombinant inbred line population derived from the XC7 × XC21 cross. These evaluations were conducted under both non-stress and drought-stress conditions. Drought stress significantly reduced grain weight per spike and grain yield per plot. Genotyping the recombinant inbred line population using the wheat 90K single nucleotide polymorphism array resulted in the identification of 131 QTLs associated with the 18 traits. Drought stress also exerted negative impacts on grain formation and filling, directly leading to reductions in grain weight per spike and grain yield per plot. Among the identified QTLs, 43 were specifically associated with drought tolerance across the 18 traits, with 6 showing direct linkages to drought tolerance in wheat. These results provide valuable insights into the genetic mechanisms governing wheat growth and development, as well as the traits contributing to the drought tolerance index. Moreover, they serve as a theoretical foundation for the development of new wheat cultivars having exceptional drought tolerance and high yield potentials under both drought-prone and drought-free conditions.
Optimizing Agroecological Measures for Climate-Resilient Olive Farming in the Mediterranean
Authors: Hrameche Oumaima , Tul Safiye , Manolikaki Ioanna , Digalaki Nektaria , Kaltsa Ioanna , Psarras Georgios , Koubouris Georgios
Published: March 2024

In order to evaluate the potential of climate change mitigation measures on soil physiochemical properties, an experiment based on the application of five agroecological practices such as the addition of composted olive-mill wastes, recycling pruning residue, cover crops, organic insect manure, and reduced soil tillage, solely or combined, was conducted over two years (2020 to 2022) in a 48-year-old olive plantation. The results showed significant increases in soil water content during the spring and summer periods for the combined treatment (compost + pruning residue + cover crops) (ALL) compared to the control (CONT) by 41.6% and 51.3%, respectively. Also, ALL expressed the highest soil organic matter (4.33%) compared to CONT (1.65%) at 0–10 cm soil depth. When comparing soil nutrient contents, ALL (37.86 mg kg−1) and cover crops (COVER) (37.21 mg kg−1) had significant increases in soil nitrate compared to CONT (22.90 mg kg−1), the lowest one. Concerning exchangeable potassium, ALL (169.7 mg kg−1) and compost (COMP) (168.7 mg kg−1) were higher than CONT (117.93 mg kg−1) at the 0–10 cm soil depth and had, respectively an increase of 100.9% and 60.7% in calcium content compared to CONT. Over the experimental period, the implementation of the five agroecological management practices resulted in enhanced soil fertility. In a long-term Mediterranean context, this study suggests that these sustainable practices would significantly benefit farmers by improving agroecosystem services, reducing reliance on synthetic fertilizers, optimizing irrigation water use, and ultimately contributing towards a circular economy.
Phylogeny and Taxonomic Revision of the Genus Melanosciadium (Apiaceae), Based on Plastid Genomes and Morphological Evidence
Authors: Jiang Qiu-Ping , Guo Xian-Lin , Zhao An-Qi , Fan Xing , Li Qing , Zhou Song-Dong , He Xing-Jin
Published: March 2024

Melanosciadium is considered a monotypic genus and is also endemic to the southwest of China. No detailed phylogenetic studies or plastid genomes have been identified in Melanosciadium. In this study, the plastid genome sequence and nrDNA sequence were used for the phylogenetic analysis of Melanosciadium and its related groups. Angelica tsinlingensis was previously considered a synonym of Hansenia forbesii. Similarly, Ligusticum angelicifolium was previously thought to be the genus Angelica or Ligusticopsis. Through field observations and morphological evidence, we believe that the two species are more similar to M. pimpinelloideum in leaves, umbel rays, and fruits. Meanwhile, we found a new species from Anhui Province (eastern China) that is similar to M. pimpinelloideum and have named it M. Jinzhaiensis. We sequenced and assembled the complete plastid genomes of these species and another three Angelica species. The genome comparison results show that M. pimpinelloideum, A. tsinlingensis, Ligusticum angelicifolium, and M. jinzhaiensis have similarities to each other in the plastid genome size, gene number, and length of the LSC and IR regions; the plastid genomes of these species are distinct from those of the Angelica species. In addition, we reconstruct the phylogenetic relationships using both plastid genome sequences and nrDNA sequences. The phylogenetic analysis revealed that A. tsinlingensis, M. pimpinelloideum, L. angelicifolium, and M. jinzhaiensis are closely related to each other and form a monophyletic group with strong support within the Selineae clade. Consequently, A. tsinlingensis and L. angelicifolium should be classified as members of the genus Melanosciadium, and suitable taxonomical treatments have been proposed. Meanwhile, a comprehensive description of the new species, M. jinzhaiensis, is presented, encompassing its habitat environment and detailed morphological traits.