bluuebunny/crossref_metadata_2025 huggingface dataset:
Word-Based Processor Structure for Montgomery Modular Multiplier Suitable for Compact IoT Edge Devices
Authors: Ibrahim Atef , Gebali Fayez
Published: January 2023

The Internet of Things (IoT) is an emerging technology that forms a huge network of different objects and intelligent devices. IoT Security is becoming more important due to the exchange of sensitive sensor data and the potential for incorporating the virtual and real worlds. IoT edge devices create serious security threats to network systems. Due to their limited resources, it is challenging to implement cryptographic protocols on these devices to secure them. To address this problem, we should perform compact implementation of cryptographic algorithms on these devices. At the heart of most cryptographic algorithms is the modular multiplication operation. Therefore, efficient implementation of this operation will have a great impact on the implementation of the whole cryptographic protocol. In this paper, we will focus on the resource and energy efficient hardware implementation of the adopted Montgomery modular multiplication algorithm over GF(2m). The main building block of the proposed word-based processor structure is a processor array that has a modular structure with local connectivity between its processing elements. The ability to manage the saving amounts of area, delay, and consumed energy is the main benefit of the suggested hardware structure. We used ASIC technology to implement the suggested word-based processor structure. The final results show an average reduction in the area of 86.3% when compared with the competitive word-based multiplier structures. Additionally, the recommended design achieves significant average savings in area-time product, power, and consumed energy of 53.7%, 83.2%, and 72.6%, receptively, over the competitive ones. The obtained results show that the provided processor structure is best suited for application in compact IoT edge devices with limited resources.
Dynamics of Evolutionary Differential Equations with Several Spatial Variables
Authors: Kushner Alexei G.
Published: January 2023

The article is devoted to a method for constructing exact and approximate solutions of evolutionary partial differential equations with several spatial variables. The method is based on the theory of completely integrable distributions. Examples of applying this method to calculating exact solutions of the generalized Kolmogorov–Petrovskii–Piskunov–Fishev equations with two space variables are given.
K-L Estimator: Dealing with Multicollinearity in the Logistic Regression Model
Authors: Lukman Adewale F. , Kibria B. M. Golam , Nziku Cosmas K. , Amin Muhammad , Adewuyi Emmanuel T. , Farghali Rasha
Published: January 2023

Multicollinearity negatively affects the efficiency of the maximum likelihood estimator (MLE) in both the linear and generalized linear models. The Kibria and Lukman estimator (KLE) was developed as an alternative to the MLE to handle multicollinearity for the linear regression model. In this study, we proposed the Logistic Kibria-Lukman estimator (LKLE) to handle multicollinearity for the logistic regression model. We theoretically established the superiority condition of this new estimator over the MLE, the logistic ridge estimator (LRE), the logistic Liu estimator (LLE), the logistic Liu-type estimator (LLTE) and the logistic two-parameter estimator (LTPE) using the mean squared error criteria. The theoretical conditions were validated using a real-life dataset, and the results showed that the conditions were satisfied. Finally, a simulation and the real-life results showed that the new estimator outperformed the other considered estimators. However, the performance of the estimators was contingent on the adopted shrinkage parameter estimators.
On the Theory of Methane Hydrate Decomposition in a One-Dimensional Model in Porous Sediments: Numerical Study
Authors: Abu-Nab Ahmed K. , Koldoba Alexander V. , Koldoba Elena V. , Poveshchenko Yury A. , Podryga Viktoriia O. , Rahimly Parvin I. , Bakeer Ahmed E.
Published: January 2023

The purpose of this paper is to present a one-dimensional model that simulates the thermo-physical processes for methane hydrate decomposition in porous media. The mathematical model consists of equations for the conservation of energy, gas, and liquid as well as the thermodynamic equilibrium equation for temperature and pressure (P−T) in the hydrate stability region. The developed model is solved numerically by using the implicit finite difference technique on the grid system, which correctly describes the appearance of phase, latency, and boundary conditions. The Newton–Raphson method was employed to solve a system of nonlinear algebraic equations after defining and preparing the Jacobean matrix. Additionally, the proposed model describes the decomposition of methane hydrate by thermal catalysis of the components that make up the medium through multiple phases in porous media. In addition, the effect of thermodynamic processes during the hydrate decomposition on the pore saturation rate with hydrates a7nd water during different time periods was studied in a one-dimensional model. Finally, in a one-dimensional model over various time intervals, t=1, 10, 50 s, the pressure and temperature distributions during the decomposition of methane hydrates are introduced and investigated. The obtained results include more accurate solutions and are consistent with previous models based on the analysis of simulations and system stability.
Artificial Neural Networking Magnification for Heat Transfer Coefficient in Convective Non-Newtonian Fluid with Thermal Radiations and Heat Generation Effects
Authors: Rehman Khalil Ur , Shatanawi Wasfi , Çolak Andaç Batur
Published: January 2023

In this study, the Casson fluid flow through an inclined, stretching cylindrical surface is considered. The flow field is manifested with pertinent physical effects, namely heat generation, viscous dissipation, thermal radiations, stagnation point flow, variable thermal conductivity, a magnetic field, and mixed convection. In addition, the flow field is formulated mathematically. The shooting scheme is used to obtain the numerical data of the heat transfer coefficient at the cylindrical surface. Further, for comparative analysis, three different thermal flow regimes are considered. In order to obtain a better estimation of the heat transfer coefficient, three corresponding artificial neural networks (ANN) models were constructed by utilizing Tan-Sig and Purelin transfer functions. It was observed that the heat transfer rate exhibits an inciting nature for the Eckert and Prandtl numbers, curvature, and heat generation parameters, while the Casson fluid parameter, temperature-dependent thermal conductivity, and radiation parameter behave oppositely. The present ANN estimation will be helpful for studies related to thermal energy storage that have Nusselt number involvements.
Silicic Acid Removal by Metal-Organic Frameworks for Silica-Scale Mitigation in Reverse Osmosis
Authors: Guo Rui , Zhang Jun , Mufanebadza Taona , Tian Xinxia , Xie Lixin , Zhao Song
Published: January 2023

Reverse osmosis (RO) membranes are susceptible to silica scaling, resulting in irreversible degradation of membrane performance. This work covered the fabrication of MIL-101(Fe) for silicic acid adsorption to alleviate the silica scaling of RO membranes. The effect of pH, mixing time and initial concentration on silicic acid adsorption of MIL-101(Fe) was appraised in detail. The adsorption experiments demonstrated that MIL-101(Fe) possessed an excellent adsorption ability for silicic acid with the maximum adsorption capacity reaching 220.1 mgSiO2·g−1. Data fitting confirmed the pseudo-second-order equation and Freundlich equation were consistent with silicic acid adsorption on MIL-101(Fe). Finally, a simulated anti-scaling experiment was carried out using a feed solution pretreated by MIL-101(Fe) adsorption, and the permeance exhibited a much lower decline after 24 h filtration, confirming that MIL-101(Fe) exhibits an excellent application potential for silica-scale mitigation in RO systems.
Improved Method Based on Retinex and Gabor for the Surface Defect Enhancement of Aluminum Strips
Authors: Zhang Qi , Tang Hongqun , Li Yong , Han Bing , Li Jiadong
Published: January 2023

Aiming at the problems of the blurred image defect contour and the surface texture of the aluminum strip suppressing defect feature extraction when collecting photos online in the air cushion furnace production line, we propose an algorithm for the surface defect enhancement and detection of aluminum strips based on the Retinex theory and Gobar filter. The Retinex algorithm can enhance the information and detail part of the image, while the Gobar algorithm can maintain the integrity of the defect edges well. The method first improves the high-frequency information of the image using a multi-scale Retinex based on a Laplacian filter, scales the original image and the enhanced image, and enhances the contrast of the image by adaptive histogram equalization. Then, the image is denoised, and texture suppressed using median filtering and morphological operations. Finally, Gobar edge detection is performed on the obtained sample images by convolving the sinusoidal plane wave and the Gaussian kernel function in the null domain and performing double-threshold segmentation to extract and refine the edges. The algorithm in this paper is compared with histogram equalization and the Gaussian filter-based MSR algorithm, and the surface defects of aluminum strips are significantly enhanced for the background. The experimental results show that the information entropy of the aluminum strip material defect image is improved from 5.03 to 7.85 in the original image, the average gradient of the image is improved from 3.51 to 9.51 in the original image, the contrast between the foreground and background is improved from 16.66 to 117.53 in the original image, the peak signal-to-noise ratio index is improved to 24.50 dB, and the integrity of the edges is well maintained while denoising. This paper’s algorithm effectively enhances and detects the surface defects of aluminum strips, and the edges of defect contours are clearer and more complete.
Hall-Petch Strengthening by Grain Boundaries and Annealing Twin Boundaries in Non-Equiatomic Ni2FeCr Medium-Entropy Alloy
Authors: Li Zhiwen , Wang Liang , Liu Chen , Zhao Junbo , Wang Binbin , Li Zhe , Luo Liangshun , Chen Ruirun , Su Yanqing , Guo Jingjie
Published: January 2023

A novel Co-free non-equiatomic Ni2FeCr medium-entropy alloy (MEA) was designed, and the Hall–Petch strengthening by grain boundaries and annealing twin boundaries was investigated. For this purpose, the alloy was prepared by cold rolling and recrystallization at 873–1323 K for 40 min–6 h. Annealing at different temperatures revealed that Ni2CrFe alloy forms a stable face-centered cubic (FCC) solid solution. Mean grain sizes (excluding annealing twin boundaries) and mean crystallite sizes (including both grain and annealing twin boundaries) were determined using the linear intercept method and the equivalent circle diameter in electron back-scattered diffraction (EBSD) soft. Tensile tests at 293 K indicated that the Hall-Petch slopes of grain sizes and crystallite sizes are 673 and 544 MPa μm1/2, respectively, and this contribution was then subtracted from the overall strength to calculate the intrinsic uniaxial lattice strength (90 MPa). Additionally, tensile tests, performed between 293 K and 873 K, revealed that the Ni2CrFe MEA has a stronger resistance to softening at high temperatures. Transmission electron microscopy of deformed specimens revealed the formation of dislocation pile-ups at annealing twin boundaries, indicating that it is also an obstacle to dislocation slip. Furthermore, the thickening of the annealing twin boundary after deformation was observed and illustrated by the interaction between different dislocations and annealing twin boundaries.
Phytochemicals and Regulation of NF-kB in Inflammatory Bowel Diseases: An Overview of In Vitro and In Vivo Effects
Authors: Laurindo Lucas Fornari , Santos Ana Rita de Oliveira dos , Carvalho Antonelly Cassio Alves de , Bechara Marcelo Dib , Guiguer Elen Landgraf , Goulart Ricardo de Alvares , Vargas Sinatora Renata , Araújo Adriano Cressoni , Barbalho Sandra Maria
Published: January 2023

Inflammatory bowel diseases (IBD) are chronic relapsing idiopathic inflammatory conditions affecting the gastrointestinal tract. They are mainly represented by two forms, ulcerative colitis (UC) and Crohn’s disease (CD). IBD can be associated with the activation of nuclear factors, such as nuclear factor-kB (NF-kB), leading to increased transcription of pro-inflammatory mediators that result in diarrhea, abdominal pain, bleeding, and many extra-intestinal manifestations. Phytochemicals can interfere with many inflammation targets, including NF-kB pathways. Thus, this review aimed to investigate the effects of different phytochemicals in the NF-kB pathways in vitro and in vivo models of IBD. Fifty-six phytochemicals were included in this study, such as curcumin, resveratrol, kaempferol, sesamol, pinocembrin, astragalin, oxyberberine, berberine hydrochloride, botulin, taxifolin, naringin, thymol, isobavachalcone, lancemaside A, aesculin, tetrandrine, Ginsenoside Rk3, mangiferin, diosgenin, theanine, tryptanthrin, lycopene, gyngerol, alantolactone, mangostin, ophiopogonin D, fisetin, sinomenine, piperine, oxymatrine, euphol, artesunate, galangin, and nobiletin. The main observed effects related to NF-kB pathways were reductions in tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-6, interferon-gamma (IFN-γ), and cyclooxygenase-2 (COX-2), and augmented occludin, claudin-1, zonula occludens-1, and IL-10 expression levels. Moreover, phytochemicals can improve weight loss, stool consistency, and rectal bleeding in IBD. Therefore, phytochemicals can constitute a powerful treatment option for IBD in humans.
Effects of Carbohydrase Supplementation on Growth Performance, Intestinal Digestive Enzymes and Flora, Glucose Metabolism Enzymes, and glut2 Gene Expression of Hybrid Grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) Fed Different CHO/L Ratio Diets
Authors: Liu Hongyu , Pan Ling , Shen Jianfei , Tan Beiping , Dong Xiaohui , Yang Qihui , Chi Shuyan , Zhang Shuang
Published: January 2023

An optimal carbohydrate-to-lipid (CHO: L) ratio facilitates fish growth and protein conservation, and carbohydrase promotes nutrient absorption. Therefore, an 8-week feeding trial was conducted to investigate the effects of carbohydrase supplementation on growth performance, intestinal digestive enzymes and flora, glucose metabolism enzymes and glut2 gene expression in juvenile hybrid grouper (Epinephelus fuscoguttatus♀× Epinephelus lanceolatus♂) fed different CHO: L ratios diets. L, M, and H represent CHO:L ratios of 0.91, 1.92 and 3.91, respectively. LE, ME, and HE represent CHO:L ratios of 0.91, 1.92, 3.91, respectively, supplemented with the same ratio of carbohydrase. Results showed that weight gain rate (WGR) and specific growth rate (SGR) reached a maximum in group M and were significantly enhanced by carbohydrase (p < 0.05). Crude lipid content decreased significantly with an increase in the dietary CHO:L ratio (p < 0.05). Significant increases in the trypsin (TRY) and amylase (AMS) activities and significant decreases in the lipase (LPS) activity were observed with increasing dietary CHO:L ratio, and the former two were significantly promoted by carbohydrase (p < 0.05). The content of liver and muscle glycogen increased significantly with the increasing dietary CHO:L ratio but decreased significantly after carbohydrase supplementation (p < 0.05). The glucokinase (GK), pyruvate kinase (PK), Phosphate 6 fructokinase-1 (PFK-1) and phosphoenolpyruvate kinase (PEPCK) activities increased significantly with increasing dietary CHO:L ratio (p < 0.05). Glut2 mRNA expression decreased significantly in liver and increased significantly in intestine with increasing dietary CHO:L ratio (p < 0.05). By linear discriminant analysis (LDA), the abundance of Alistipe was significantly higher in Group ME than in Group M. These results suggested that hybrid grouper can only moderately utilize dietary carbohydrate and lipid in diet, and a certain amount of high glycemic lipids occurred when fed with high-carbohydrate diets. By the weight gain for basis, the supplementation of carbohydrase in Group H with amylase, glycosylase, and pullulanase in a 1:1:1 ratio effectively lowered glycemic lipids, promoted the growth of grouper, digestive enzymes activities and carbohydrate metabolic enzyme, and glut2 gene expression in intestine, effectively balancing the negative effects of high-carbohydrate diet and improving the utilization of carbohydrate.
Metabolite Profiling of Wheat Response to Cultivar Improvement and Nitrogen Fertilizer
Authors: Zhao Fulin , Wang Yifan , Hu Jiayu , Shi Shaolei , Zhang Hongyan , Wang Yang , Ye Youliang
Published: January 2023

Both genetic improvement and the application of N fertilizer increase the quality and yields of wheat. However, the molecular kinetics that underlies the differences between them are not well understood. In this study, we performed a non-targeted metabolomic analysis on wheat cultivars from different release years to comprehensively investigate the metabolic differences between cultivar and N treatments. The results revealed that the plant height and tiller number steadily decreased with increased ears numbers, whereas the grain number and weight increased with genetic improvement. Following the addition of N fertilizer, the panicle numbers and grain weights increased in an old cultivar, whereas the panicle number and grain number per panicle increased in a modern cultivar. For the 1950s to 2010s cultivar, the yield increases due to genetic improvements ranged from −1.9% to 96.7%, whereas that of N application ranged from 19.1% to 81.6%. Based on the untargeted metabolomics approach, the findings demonstrated that genetic improvements induced 1.4 to 7.4 times more metabolic alterations than N fertilizer supply. After the addition of N, 69.6%, 29.4%, and 33.3% of the differential metabolites were upregulated in the 1950s, 1980s, and 2010s cultivars, respectively. The results of metabolic pathway analysis of the identified differential metabolites via genetic improvement indicated enrichment in 1-2 KEGG pathways, whereas the application of N fertilizer enriched 2–4 pathways. Our results provide new insights into the molecular mechanisms of wheat quality and grain yield developments.
Physical Inactivity, Metabolic Syndrome and Prostate Cancer Diagnosis: Development of a Predicting Nomogram
Authors: De Nunzio Cosimo , Brassetti Aldo , Cancrini Fabiana , Prata Francesco , Cindolo Luca , Sountoulides Petros , Toutziaris Chrysovalantis , Gacci Mauro , Lombardo Riccardo , Cicione Antonio , Tema Giorgia , Schips Luigi , Simone Giuseppe , Serni Sergio , Tubaro Andrea
Published: January 2023

Insufficient physical activity (PA) may be a shared risk factor for the development of both metabolic syndrome (MetS) and prostate cancer (PCa). To investigate this correlation and to develop a nomogram able to predict tumor diagnosis. Between 2016 and 2018, a consecutive series of men who underwent prostate biopsy at three institutions were prospectively enrolled. PA was self-assessed by patients through the Physical Activity Scale for the Elderly (PASE) questionnaire; MetS was assessed according to Adult Treatment Panel III criteria. A logistic regression analyses was used to identify predictors of PCa diagnosis and high-grade disease (defined as International Society of Uro-Pathology grade >2 tumors). A nomogram was then computed to estimate the risk of tumor diagnosis. A total of 291 patients were enrolled; 17.5% of them (n = 51) presented with MetS. PCa was diagnosed in 110 (38%) patients overall while 51 presented high-grade disease. At multivariable analysis, age (OR 1.04; 95%CI: 1.00–1.08; p = 0.048), prostate volume (PV) (OR 0.98; 95%CI: 0.79–0.99; p = 0.004), suspicious digital rectal examination (OR 2.35; 95%CI: 1.11–4.98; p = 0.02), total PSA value (OR 1.12; 95%CI: 1.05–1.2; p < 0.001), and PASE score (OR 0.99; 95%CI: 0.98–0.99; p = 0.01) were independent predictors of tumor diagnosis. The latter two also predicted high-grade PCa. MetS was not associated with PCa diagnosis and aggressiveness. The novel nomogram displayed fair discrimination for PCa diagnosis (AUC = 0.76), adequate calibration (p > 0.05) and provided a net benefit in the range of probabilities between 20% and 90%. reduced PA was associated with an increased risk of PCa diagnosis and high-grade disease. Our nomogram could improve the selection of patients scheduled for prostate biopsy at increased risk of PCa.
Mitochondrial and Endoplasmic Reticulum Stress Trigger Triglyceride Accumulation in Models of Parkinson’s Disease Independent of Mutations in MAPT
Authors: Fernandes Hugo J. R. , Kent Josh P. , Bruntraeger Michaela , Bassett Andrew R. , Koulman Albert , Metzakopian Emmanouil , Snowden Stuart G.
Published: January 2023

The metabolic basis of Parkinson’s disease pathology is poorly understood. However, the involvement of mitochondrial and endoplasmic reticulum stress in dopamine neurons in disease aetiology is well established. We looked at the effect of rotenone- and tunicamycin-induced mitochondrial and ER stress on the metabolism of wild type and microtubule-associated protein tau mutant dopamine neurons. Dopamine neurons derived from human isolated iPSCs were subjected to mitochondrial and ER stress using RT and TM, respectively. Comprehensive metabolite profiles were generated using a split phase extraction analysed by reversed phase lipidomics whilst the aqueous phase was measured using HILIC metabolomics. Mitochondrial and ER stress were both shown to cause significant dysregulation of metabolism with RT-induced stress producing a larger shift in the metabolic profile of both wild type and MAPT neurons. Detailed analysis showed that accumulation of triglycerides was a significant driver of metabolic dysregulation in response to both stresses in both genotypes. Whilst the consequence is similar, the mechanisms by which triglyceride accumulation occurs in dopamine neurons in response to mitochondrial and ER stress are very different. Thus, improving our understanding of how these mechanisms drive the observed triglyceride accumulation can potentially open up new therapeutic avenues.
Design and Fabrication of a Low-Cost, Multiband and High Gain Square Tooth-Enabled Metamaterial Superstrate Microstrip Patch Antenna
Authors: Aliqab Khaled , Lavadiya Sunil , Alsharari Meshari , Armghan Ammar , Daher Malek G. , Patel Shobhit K.
Published: January 2023

The manuscript represents a novel square tooth-enabled superstrate metamaterial loaded microstrip patch antenna for the multiple frequency band operation. The proposed tooth-based metamaterial antenna provides better gain and directivity. Four antenna structures are numerically investigated for the different geometry of the patch and tooth. These proposed structures are simulated, fabricated, measured, and compared for the frequency range of 3 GHz to 9 GHz. The electrical equivalent model of the split-ring resonator is also analyzed in the manuscript. The comparative analysis of all of the proposed structures has been carried out, in terms of several bands, reflectance response, VSWR, gain and bandwidth. The results are compared with previously published works. The effects are simulated using a high-frequency structure simulator tool with the finite element method. The measured and fabricated results are compared for verification purposes. The proposed structure provides seven bands of operation and 8.57 dB of gain. It is observed that the proposed design offers the multiple frequency band operation with a good gain. The proposed tooth-based metamaterial antenna suits applications, such as the surveillance radar, satellite communication, weather monitoring and many other wireless devices.
Tick Diversity and Distribution of Hard (Ixodidae) Cattle Ticks in South Africa
Authors: Makwarela Tsireledzo G. , Nyangiwe Nkululeko , Masebe Tracy , Mbizeni Sikhumbuzo , Nesengani Lucky T. , Djikeng Appolinaire , Mapholi Ntanganedzeni O.
Published: January 2023

Ticks are amongst the important ectoparasites where livestock are concerned, as they adversely affect the animals through bloodsucking. In tropical and subtropical countries, they transmit pathogens such as babesiosis, theileriosis, ehrlichiosis, and anaplasmosis in cattle, causing a reduction in production rate and significant concomitant economic losses. Ticks affect 80% of the cattle population across the world, with an estimated economic loss of USD 20–30 billion per year. In South Africa, economic losses in the livestock industry caused by ticks and tick-borne diseases are estimated to exceed USD 33 million per year (ZAR 500 million). There are seven major genera of ixodid ticks in Southern Africa (i.e., Amblyomma, Dermacentor, Haemaphysalis, Hyalomma, Ixodes and Rhipicephalus). The environment in which a tick lives is made up of all the various biological and abiotic factors that are either necessary or unnecessary for its life. The areas where various ticks have been found have been documented in many publications. Using these data, maps of possible species’ habitats can be made. Historical records on tick distribution may be incorrect due to identification mistakes or a change in the tick’s name. All the sources used to generate the maps for this review were unpublished and came from a wide range of sources. To identify tick species and the accompanying microbial ecosystems, researchers are increasingly adopting tick identification methods including 16S and 18S rDNA gene sequencing. Indeed, little is known about the genetic alterations that give important traits, including the predilection for tick hosts, transmission, and acaricide resistance. Opportunities for exploring these changes in tick populations and subpopulations are provided by advancements in omics technologies. The literature on the variety of ixodid ticks, their direct and indirect effects, and control methods in South Africa is compiled in this review.
Molecular and Source-Specific Profiling of Hospital Staphylococcus aureus Reveal Dominance of Skin Infection and Age-Specific Selections in Pediatrics and Geriatrics
Authors: Said Kamaleldin B. , Alghasab Naif Saad , Alharbi Mohammed S. M. , Alsolami Ahmed , Saleem Mohd , Alhallabi Sulaf A. , Alafnan Shahad F. , Khaja Azharuddin Sajid Syed , Taha Taha E. ,   on behalf of the Ha’il COM Research Unit Group
Published: January 2023

Staphylococcus aureus is a major human-associated pathogen that causes a wide range of clinical infections. However, the increased human dynamics and the changing epidemiology of the species have made it imperative to understand the population structure of local ecotypes, their transmission dynamics, and the emergence of new strains. Since the previous methicillin-resistant S. aureus (MRSA) pandemic, there has been a steady increase in global healthcare-associated infections involving cutaneous and soft tissue and resulting in high morbidities and mortalities. Limited data and paucity of high-quality evidence exist for many key clinical questions about the pattern of S. aureus infections. Using clinical, molecular, and epidemiological characterizations of isolates, hospital data on age and infection sites, as well as antibiograms, we have investigated profiles of circulating S. aureus types and infection patterns. We showed that age-specific profiling in both intensive care unit (ICU) and non-ICU revealed highest infection rates (94.7%) in senior-patients > 50 years; most of which were MRSA (81.99%). However, specific distributions of geriatric MRSA and MSSA rates were 46.5% and 4.6% in ICU and 35.48% and 8.065% in non-ICU, respectively. Intriguingly, the age groups 0–20 years showed uniquely similar MRSA patterns in ICU and non-ICU patients (13.9% and 9.7%, respectively) and MSSA in ICU (11.6%). The similar frequencies of both lineages in youth at both settings is consistent with their increased socializations and gathering strongly implying carriage and potential evolutionary replacement of MSSA by MRSA. However, in age groups 20–50 years, MRSA was two-fold higher in non-ICU (35%) than ICU (18.6%). Interestingly, a highly significant association was found between infection-site and age-groups (p-value 0.000). Skin infections remained higher in all ages; pediatrics 32.14%, adults 56%, and seniors 25% while respiratory infections were lower in pediatrics (14.3%) and adults (17%) while it was highest in seniors (38%). Blood and “other” sites in pediatrics were recorded (28.6%; 25%, respectively), and were slightly lower in adults (18.6%; 8.6%) and seniors (14%; 22.8%), respectively. Furthermore, a significant association existed between infection-site and MRSA (Chi-Square Test, p-value 0.002). Thus, the common cutaneous infections across all age-groups imply that skin is a significant reservoir for endogenous infections; particularly, for geriatrics MRSA. These findings have important clinical implications and in understanding S. aureus profiles and transmission dynamics across different age groups that is necessary for strategic planning in patient management and infection control.
Diversity, Distribution, and Development of Hyperparasitic Microsporidia in Gregarines within One Super-Host
Authors: Frolova Ekaterina V. , Paskerova Gita G. , Smirnov Alexey V. , Nassonova Elena S.
Published: January 2023

Metchnikovellids (Microsporidia: Metchnikovellida) are poorly studied hyperparasitic microsporidia that live in gregarines inhabiting the intestines of marine invertebrates, mostly polychaetes. Our recent studies showed that diversity of metchnikovellids might be significantly higher than previously thought, even within a single host. Four species of metchnikovellids were found in the gregarines inhabiting the gut of the polychaete Pygospio elegans from littoral populations of the White and Barents Seas: the eugregarine Polyrhabdina pygospionis is the host for Metchnikovella incurvata and M. spiralis, while the archigregarine Selenidium pygospionis is the host for M. dogieli and M. dobrovolskiji. The most common species in the White Sea is M. incurvata, while M. dobrovolskiji prevails in the Barents Sea. Gregarines within a single worm could be infected with different metchnikovellid species. However, co-infection of one and the same gregarine with several species of metchnikovellids has never been observed. The difference in prevalence and intensity of metchnikovellid invasion apparently depends on the features of the life cycle and on the development strategies of individual species.
A 63-kDa Periplasmic Protein of the Endonuclear Symbiotic Bacterium Holospora obtusa Secreted to the Outside of the Bacterium during the Early Infection Process Binds Weakly to the Macronuclear DNA of the Host Paramecium caudatum
Authors: Fujishima Masahiro , Kawano Hideaki , Miyakawa Isamu
Published: January 2023

The Gram-negative bacterium Holospora obtusa is a macronucleus-specific symbiont of the ciliate Paramecium caudatum. It is known that an infection of this bacterium induces high level expressions of the host hsp60 and hsp70 genes, and the host cell acquires both heat-shock and high salt resistances. In addition, an infectious form of H. obtusa-specific 63-kDa periplasmic protein with a DNA-binding domain in its amino acid sequence is secreted into the host macronucleus after invasion into the macronucleus and remain within the nucleus. These facts suggest that binding of the 63-kDa protein to the host macronuclear DNA causes changes in the host gene expressions and enhances an environmental adaptability of the host cells. This 63-kDa protein was renamed as periplasmic region protein 1 (PRP1) to distinguish it from other proteins with similar molecular weights. To confirm whether PRP1 indeed binds to the host DNA, SDS-DNA PAGE and DNA affinity chromatography with calf thymus DNA and P. caudatum DNA were conducted and confirmed that PRP1 binds weakly to the P. caudatum DNA with a monoclonal antibody raised for the 63-kDa protein.
Interaction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence: Phagocytosis and Apoptosis
Authors: Li Siqi , Wang Chunsheng , Tang Yan-Dong , Qin Lei , Chen Tianfeng , Wang Shanghui , Bai Yuanzhe , Cai Xuehui , Wang Shujie
Published: January 2023

Streptococcus suis is an important swine bacterial pathogen that activates macrophages to secrete inflammatory cytokines. Primary porcine alveolar macrophages (PAMs) are inconvenient to obtain, but it is unknown whether immortalized PAM-Tang cells can replace them as a better cell model for the study of the interaction between S. suis and macrophages. In this study, the phagocytic integrity, polarization, and pro-inflammatory cytokine secretion of PAM-Tang cells were confirmed by live-cell imaging, electron microscopy, confocal microscopy, and ELISA. Interestingly, the S. suis serotype 9 avirulent strain W7119 induced higher levels of adhesion and pro-inflammatory cytokines in PAM-Tang cells than the S. suis serotype 2 virulent strain 700794. Prolonged incubation with S. suis caused more cytotoxic cell damage, and the virulent strain induced higher levels of cytotoxicity to PAM-Tang cells. The virulent strain also induced higher levels of apoptosis in PAM-Tang cells, as shown by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay. In addition, it is the first report of virulent and avirulent S. suis inducing PAM-Tang polarization towards pro-inflammatory M1 macrophages and p53- and caspase-dependent apoptosis in PAMs. Taken together, this study contributes to a better understand of interactions between macrophages and S. suis isolates of different virulence, and confirms that PAM-Tang cells provide a long-term, renewable resource for investigating macrophage infections with bacteria.
Antibiotic-Resistant Bacteria, Antimicrobial Resistance Genes, and Antibiotic Residue in Food from Animal Sources: One Health Food Safety Concern
Authors: Qamar Muhammad Usman , Aatika  , Chughtai Muhammad Ismail , Ejaz Hasan , Mazhari Bi Bi Zainab , Maqbool Uzma , Alanazi Awadh , Alruwaili Yasir , Junaid Kashaf
Published: January 2023

Antibiotic-resistant bacteria causing foodborne serious illnesses can be found in contaminated food. Therefore, this study aimed to identify the pathogens, genes, and antimicrobial residues present in raw milk and meat. We collected 40 raw milk and 40 beef samples using the aseptic method from various parts of the Faisalabad metropolis, Pakistan. The samples were cultured on blood, MacConkey, and UTI chrome agar. The VITEK 2 compact system was used for microbial identification and determination of minimum inhibitory concentrations. Antimicrobial resistance genes for extended-spectrum β-lactamases, methicillin resistance in Staphylococcus aureus, and carbapenem resistance were identified using molecular techniques. ELISA was used to determine the tetracycline residue level in each sample. The beef samples showed polymicrobial contamination with 64 bacterial isolates, with Escherichia coli (29; 45.3%) and Klebsiella pneumoniae (11; 17.1%) predominating. The milk samples showed polymicrobial contamination with 73 bacterial isolates, with E. coli (22; 30%), K. pneumoniae (12; 16.4%), and S. aureus (10; 13.6%) forming the majority. Twenty-eight (43.7%) isolates from beef harbored tet genes, nineteen (29.6%) blaCTX-M, and fourteen (21.8%) blaNDM-1, and twenty-six (35.6%) isolates from milk harbored tet genes, nineteen (26%) blaTEM and blaCTX-M, and three (4%) blaNDM-1. Twenty-two (55%) each of the beef and milk samples exceeded the maximum residue limit for tetracycline. Polymicrobial contamination by bacteria possessing blaCTX-M, blaTEM, blaNDM-1, blaOXA, mecA, and tet genes was identified in food samples. The high tetracycline residue levels pose a serious health risk to consumers.
Base Metals Extraction from Printed Circuit Boards by Pressure Acid Leaching
Authors: Martinez-Ballesteros Guadalupe , Valenzuela-Garcia Jesus Leobardo , Gomez-Alvarez Agustin , Encinas-Romero Martin Antonio , Mejia-Zamudio Flerida Adriana , Rosas-Durazo Aaron de Jesus
Published: January 2023

Printed circuit boards (PCBs) are a valuable source of raw materials for metal recycling considering that base metal concentration analyses have confirmed that PCB powders are multimetallic in nature and contain high concentrations of Cu, Zn, Ni, and Fe. Given that minerals are not renewable resources, these metals can be recycled through hydrometallurgical processes. In this study, we determined that 2 M sulfuric acid, 0.55 MPa oxygen pressure, and a temperature of 90 °C represent the optimal conditions for leaching of Cu, Zn, and Ni of PCBs, obtaining the highest observed values of recovery of greater than 90% for Zn and 98% for Cu and Ni. The characterization of PCBs by SEM–EDS analyses showed that plates mainly consist of Cu, Ni, and Zn. PCBs can be seen as a potential secondary resource for the recovery of copper, nickel, and zinc. The best potential and pH conditions for the extraction of Cu, Zn, and Ni were also determined on the basis of thermodynamic diagrams.
Ensemble Tree Model for Long-Term Rockburst Prediction in Incomplete Datasets
Authors: Liu Huanxin , Zhao Guoyan , Xiao Peng , Yin Yantian
Published: January 2023

The occurrence of rockburst can seriously impact the construction and production of deep underground engineering. To prevent rockburst, machine learning (ML) models have been widely employed to predict rockburst based on some related variables. However, due to the costs and complicated geological conditions, complete datasets to evaluate rockburst cannot always be obtained in rock engineering. To fill this limitation, this study proposed an ensemble tree model suitable for incomplete datasets, i.e., the histogram gradient boosting tree (HGBT), to build intelligent models for rockburst prediction. Three hundred fourteen rockburst cases were employed to develop the HGBT model. The hunger game search (HGS) algorithm was implemented to optimize the HGBT model. The established HGBT model had an excellent testing performance (accuracy of 88.9%). An incomplete database with missing values was applied to compare the performances of HGBT and other ML models (random forest, artificial neural network, and so on). HGBT received an accuracy of 78.8% in the incomplete database, and its capacity was better than that of other ML models. Additionally, the importance of input variables in the HGBT model was analyzed. Finally, the feasibility of the HGBT model was validated by rockburst cases from Sanshandao Gold Mine, China.
Obtaining 2,3-Dihydrobenzofuran and 3-Epilupeol from Ageratina pichinchensis (Kunth) R.King & Ho.Rob. Cell Cultures Grown in Shake Flasks under Photoperiod and Darkness, and Its Scale-Up to an Airlift Bioreactor for Enhanced Production
Authors: Sánchez-Ramos Mariana , Marquina-Bahena Silvia , Alvarez Laura , Bernabé-Antonio Antonio , Cabañas-García Emmanuel , Román-Guerrero Angélica , Cruz-Sosa Francisco
Published: January 2023

Ageratina pichinchensis (Kunth) R.King & Ho.Rob. is a plant used in traditional Mexican medicine, and some biotechnological studies have shown that its calluses and cell suspension cultures can produce important anti-inflammatory compounds. In this study, we established a cell culture of A. pichinchensis in a 2 L airlift bioreactor and evaluated the production of the anti-inflammatory compounds 2,3-dihydrobenzofuran (1) and 3-epilupeol (2). The maximum biomass production (11.90 ± 2.48 g/L) was reached at 11 days of culture and cell viability was between 80% and 90%. Among kinetic parameters, the specific growth rate (µ) was 0.2216 days−1 and doubling time (td) was 3.13 days. Gas chromatography coupled with mass spectrometry (GC-MS) analysis of extracts showed the maximum production of compound 1 (903.02 ± 41.06 µg/g extract) and compound 2 (561.63 ± 10.63 µg/g extract) at 7 and 14 days, respectively. This study stands out for the significant production of 2,3-dihydrobenzofuran and 3-epilupeol and by the significant reduction in production time compared to callus and cell suspension cultures, previously reported. To date, these compounds have not been found in the wild plant, i.e., its production has only been reported in cell cultures of A. pichinchensis. Therefore, plant cell cultured in an airlift reactor can be an alternative for the improved production of these anti-inflammatory compounds.
Modeling of Enhanced Polar Magneto-Optic Kerr Effect by Surface Plasmons in Au Bowtie Arrays
Authors: Liu Jingyi , Long Lianchun , Yang Yang
Published: January 2023

The weak magneto-optical (MO) signal of traditional MO materials is indeed an important issue for their further practical applications. Although many strategies have been proposed to improve the MO effect, hybridization with noble metal nanostructures is a promising route in recent years due to the high localized-surface plasmon resonances (LSPR) effect. A new magneto-optical surface plasmon resonance (MOSPR) structure hybrid with Au bowtie arrays is proposed to increase the measuring range of the polar magneto-optical Kerr effect (PMOKE) and the quality factor through the LSPR effect. It is verified by a numerical simulation of the finite element method (FEM). The optimized parameters were found by modulating the shape and geometric dimensions. Owing to the significant LSPR from the Au bowties, a PMOKE amplification signal spectrum with narrow linewidth, and a high amplitude with high-sensing performance was achieved. Compared with the bare magnetic film alone, by optimizing the relevant parameters of the LSPR structure, the maximum signal increases 3255 times, and the quality factor can be greatly improved, which would provide important guidance and help for the practical application of MO devices.
Interface Engineering Enables High-Performance Sb Anode for Sodium Storage
Authors: Liu Chang , Fu Xin , Liao Shuzhen , Zou Guoqiang , Yang Hai
Published: January 2023

Heterointerface engineering has been verified to be an effective approach to enhance the energy density of alkali-ion batteries by resolving inherent shortcomings of single materials. However, the rational construction of heterogeneous composite with abundant heterogeneous interfaces for sodium-ion batteries (SIBs) is still a significant challenge. Herein, inspired by density functional theory calculations, interface engineering can greatly decrease the energy bandgap and migration barrier of Na ions in Sb and Na3Sb phases, as well as enhance the mechanical properties. A porous heterointerface MOFC–Sb is fabricated by utilizing MOF-C as a support and buffer, exhibiting excellent electrochemical performances for sodium storage. The MOF-C–Sb anode with its rich heterointerface presents an improved electrochemical performance of 540.5 mAh g−1 after 100 cycles at 0.1 A g−1, and 515.9 mAh g−1 at 1.6 A g−1 in term of sodium storage, efficiently resolving the serious volume expansion issues of metal Sb. These results indicate the structural superiority of heterointerface-engineered structure and afford valuable information for the rational design and construction of Sb-based anode materials for high-performance electrochemical energy storage.
Formation of a Nanorod-Assembled TiO2 Actinomorphic-Flower-like Microsphere Film via Ta Doping Using a Facile Solution Immersion Method for Humidity Sensing
Authors: Mohamed Zahidi Musa , Mamat Mohamad Hafiz , Subki A Shamsul Rahimi A , Abdullah Mohd Hanapiah , Hassan Hamizura , Ahmad Mohd Khairul , Bakar Suriani Abu , Mohamed Azmi , Ohtani Bunsho
Published: January 2023

This study fabricated tantalum (Ta)-doped titanium dioxide with a unique nanorod-assembled actinomorphic-flower-like microsphere structured film. The Ta-doped TiO2 actinomorphic-flower-like microsphere (TAFM) was fabricated via the solution immersion method in a Schott bottle with a home-made improvised clamp. The samples were characterised using FESEM, HRTEM, XRD, Raman, XPS, and Hall effect measurements for their structural and electrical properties. Compared to the undoped sample, the rutile-phased TAFM sample had finer nanorods with an average 42 nm diameter assembled to form microsphere-like structures. It also had higher oxygen vacancy sites, electron concentration, and mobility. In addition, a reversed double-beam photoacoustic spectroscopy measurement was performed for TAFM, revealing that the sample had a high electron trap density of up to 2.5 μmolg−1. The TAFM showed promising results when employed as the resistive-type sensing film for a humidity sensor, with the highest sensor response of 53,909% obtained at 3 at.% Ta doping. Adding rGO to 3 at.% TAFM further improved the sensor response to 232,152%.
Preparation of Heterojunctions Based on Cs3Bi2Br9 Nanocrystals and g-C3N4 Nanosheets for Photocatalytic Hydrogen Evolution
Authors: Medina-Llamas María , Speltini Andrea , Profumo Antonella , Panzarea Francesca , Milella Antonella , Fracassi Francesco , Listorti Andrea , Malavasi Lorenzo
Published: January 2023

Heterojunctions based on metal halide perovskites (MHPs) are promising systems for the photocatalytic hydrogen evolution reaction (HER). In this work, we coupled Cs3Bi2Br9 nanocrystals (NCs), obtained by wet ball milling synthesis, with g-C3N4 nanosheets (NSs), produced by thermal oxidation of bulk g-C3N4, in air. These methods are reproducible, inexpensive and easy to scale up. Heterojunctions with different loadings of Cs3Bi2Br9 NCs were fully characterised and tested for the HER. A relevant improvement of H2 production with respect to pristine carbon nitride was achieved at low NCs levels reaching values up to about 4600 µmol g−1 h−1. This work aims to provide insights into the synthesis of inexpensive and high-performing heterojunctions using MHP for photocatalytic applications.
Highly Efficient Energy Transfer from Silicon to Erbium in Erbium-Hyperdoped Silicon Quantum Dots
Authors: Wang Kun , He Qiang , Yang Deren , Pi Xiaodong
Published: January 2023

Erbium-doped silicon (Er-doped Si) materials hold great potential for advancing Si photonic devices. For Er-doped Si, the efficiency of energy transfer (ηET) from Si to Er3+ is crucial. In order to achieve high ηET, we used nonthermal plasma to synthesize Si quantum dots (QDs) hyperdoped with Er at the concentration of ~1% (i.e., ~5 × 1020 cm−3). The QD surface was subsequently modified by hydrosilylation using 1-dodecene. The Er-hyperdoped Si QDs emitted near-infrared (NIR) light at wavelengths of ~830 and ~1540 nm. An ultrahigh ηET (~93%) was obtained owing to the effective energy transfer from Si QDs to Er3+, which led to the weakening of the NIR emission at ~830 nm and the enhancement of the NIR emission at ~1540 nm. The coupling constant (γ) between Si QDs and Er3+ was comparable to or greater than 1.8 × 10−12 cm3·s−1. The temperature-dependent photoluminescence and excitation rate of Er-hyperdoped Si QDs indicate that strong coupling between Si QDs and Er3+ allows Er3+ to be efficiently excited.