bluuebunny/crossref_metadata_2025 huggingface dataset:
Alternative Methods to Current In Vivo Procedures to Address the 3Rs Tenet in Rabies Proficiency Testing
Authors: Zorzan Maira , Gourlaouen Morgane , Leopardi Stefania , De Benedictis Paola
Published: August 2022

Canine rabies is responsible for an estimated 59,000 human deaths every year. In an attempt to reach the ZeroBy30 goal, robust disease surveillance coupled with improved diagnostics play a paramount role in ensuring reliable data and gradually attesting rabies control advancements. In this context, proficiency testing is organized to harmonize rabies diagnostic capacities. In most exercises, rabies-positive samples consist of brains collected from intracerebrally inoculated mice. This procedure causes distress and severe suffering to animals, raising important ethical concerns that can no longer be ignored. In the last decades, the 3Rs tenet (Replace, Reduce, Refine) has been successfully implemented in several scientific areas, and we strongly support its application in the framework of rabies proficiency testing. Here, we discuss cell-based technologies as innovative sustainable in vitro candidate systems to replace in vivo experiments for the production of proficiency testing samples. The application of these alternative methods can allow completely in vitro or ex vivo production of rabies proficiency testing panels, which would represent an important replacement or reduction/refinement for current in vivo procedures.
Role of Genetic Polymorphism Present in Macrophage Activation Syndrome Pathway in Post Mortem Biopsies of Patients with COVID-19
Authors: Zanchettin Aline Cristina , Barbosa Leonardo Vinicius , Dutra Anderson Azevedo , Prá Daniele Margarita Marani , Pereira Marcos Roberto Curcio , Stocco Rebecca Benicio , Martins Ana Paula Camargo , Vaz de Paula Caroline Busatta , Nagashima Seigo , de Noronha Lucia , Machado-Souza Cleber
Published: August 2022

COVID-19 is a viral disease associated with an intense inflammatory response. Macrophage Activation Syndrome (MAS), the complication present in secondary hemophagocytic lymphohistiocytosis (sHLH), shares many clinical aspects observed in COVID-19 patients, and investigating the cytolytic function of the responsible cells for the first line of the immune response is important. Formalin-fixed paraffin-embedded lung tissue samples obtained by post mortem necropsy were accessed for three groups (COVID-19, H1N1, and CONTROL). Polymorphisms in MAS cytolytic pathway (PRF1; STX11; STXBP2; UNC13D and GZMB) were selected and genotyping by TaqMan® assays (Thermo Fisher Scientific, MA, USA) using Real-Time PCR (Applied Biosystems, MA USA). Moreover, immunohistochemistry staining was performed with a monoclonal antibody against perforin, CD8+ and CD57+ proteins. Histopathological analysis showed high perforin tissue expression in the COVID-19 group; CD8+ was high in the H1N1 group and CD57+ in the CONTROL group. An association could be observed in two genes related to the cytolytic pathway (PRF1 rs885822 G/A and STXBP2 rs2303115 G/A). Furthermore, PRF1 rs350947132 was associated with increased immune tissue expression for perforin in the COVID-19 group. The genotype approach could help identify patients that are more susceptible, and for this reason, our results showed that perforin and SNPs in the PRF1 gene can be involved in this critical pathway in the context of COVID-19.
COVID-19 Severity and Mortality in Two Pandemic Waves in Poland and Predictors of Poor Outcomes of SARS-CoV-2 Infection in Hospitalized Young Adults
Authors: Ziuzia-Januszewska Laura , Januszewski Marcin , Sosnowska-Nowak Joanna , Janiszewski Mariusz , Dobrzyński Paweł , Jakimiuk Alicja A. , Jakimiuk Artur J.
Published: August 2022

SARS-CoV-2 variants pose a significant threat to global public health. However, their influence on disease severity, especially among young adults who may exhibit different clinical characteristics, is debatable. In this retrospective study of 229 young adults hospitalized with COVID-19, we investigated the differences between Poland’s second and third waves of the pandemic. To identify potential predictors of severe COVID-19 in young adults, we analyzed patient characteristics and laboratory findings between survivors and non-survivors and we performed logistic regression to assess the risk of death, mechanical ventilation, and intensive care unit treatment. We found no increase in COVID-19 severity comparing the third and second waves of the pandemic, indicating that the alpha variant had no influence on disease severity. In addition, we found that factors, such as obesity, comorbidities, lung involvement, leukocytosis, neutrophilia, lymphopenia, higher IG count, the neutrophil-to-lymphocyte ratio, C-reactive protein, procalcitonin, interleukin-6, D-Dimer, lactate dehydrogenase, high-sensitive troponin I, creatine kinase-myocardial band, myoglobin, N-terminal-pro-B-type natriuretic peptide, creatinine, urea and gamma-glutamyl transferase, lower estimated glomerular filtration rate, albumin, calcium and vitamin D3, possibly a decrease in red blood cell counts, hemoglobin and hematocrit, and an increase in creatine kinase during hospitalization may be associated with poor outcomes of COVID-19.
Long-Term Outcomes after Vaccine-Induced Thrombotic Thrombocytopenia
Authors: Panagiota Victoria , Dobbelstein Christiane , Werwitzke Sonja , Ganser Arnold , Cooper Nina , Sachs Ulrich J. , Tiede Andreas
Published: August 2022

Vaccine-induced thrombotic thrombocytopenia (VITT), or thrombosis with thrombocytopenia syndrome (TTS), is a rare but serious complication of adenovirus-based vaccines against severe respiratory syndrome coronavirus 2 (SARS-CoV-2). Observation of long-term outcomes is important to guide treatment of affected patients. This single-center consecutive cohort study included all patients diagnosed based on (1) vaccination 4 to 21 days before symptom onset, (2) signs or symptoms of venous or arterial thrombosis, (3) thrombocytopenia < 150/nL, (4) positive anti-platelet factor 4 (PF4) antibody, and (5) elevated D-Dimer > 4 times the upper limit of normal. Nine patients were enrolled. Acute management consisted of parenteral anticoagulants, corticosteroids, intravenous immunoglobulin (IVIG), and/or eculizumab. Eculizumab was successfully used in two patients with recurrent thromboembolic events after IVIG. Direct oral anticoagulants were given after hospital discharge. Median follow-up duration was 300 days (range 153 to 380). All patients survived the acute phase of the disease and were discharged from hospital. One patient died from long-term neurological sequelae of cerebral venous sinus thrombosis 335 days after diagnosis. Eight out of nine patients were alive at last follow-up, and seven had fully recovered. Anti-PF4 antibodies remained detectable for at least 12 weeks after diagnosis, and D-Dimer remained elevated in some patients despite oral anticoagulation. No recurrent thromboembolic events, other signs of VITT relapse, or bleeding complications occurred after discharge. In conclusion, VITT appears to be a highly prothrombotic condition. IVIG is not always successful, and eculizumab may be considered a rescue agent. Long-term management with direct oral anticoagulants appears to be safe and effective.
Case Series of Myocarditis Following mRNA COVID Vaccine Compared to Pediatric Multisystem Inflammatory Syndrome: Multicenter Retrospective Study
Authors: Butbul Aviel Yonatan , Hashkes Philip J. , Dizitzer Yotam , Inbar Kanteman , Berkun Yackov , Eisenstein Eli M. , Hamad Saied Mohamad , Goldzweig Ofra , Heshin-Bekenstein Merav , Ling Eduard , Feldon Michal , Tal Rotem , Pinchevski-Kadir Shiran , Tirosh Irit , Harel Liora , Amarilyo Gil , Kaidar Kfir
Published: August 2022

Introduction: Since the development of COVID-19 vaccines, more than 4.8 billion people have been immunized worldwide. Soon after vaccinations were initiated, reports on cases of myocarditis following the second vaccine dose emerged. This study aimed to report our experience with adolescent and young adults who developed post-COVID-19 vaccine myocarditis and to compare these patients to a cohort of patients who acquired pediatric inflammatory multisystem syndrome (PIMS/PIMS-TS) post-COVID-19 infection. Methods: We collected reported cases of patients who developed myocarditis following COVID-19 vaccination (Pfizer mRNA BNT162b2) from all pediatric rheumatology centers in Israel and compared them to a cohort of patients with PIMS. Results: Nine patients with post-vaccination myocarditis were identified and compared to 78 patients diagnosed with PIMS. All patients with post-vaccination myocarditis were males who developed symptoms following their second dose of the vaccine. Patients with post-vaccination myocarditis had a shorter duration of stay in the hospital (mean 4.4 ± 1.9 vs. 8.7 ± 4.7 days) and less myocardial dysfunction (11.1% vs. 61.5%), and all had excellent outcomes as compared to the chronic changes among 9.2% of the patients with PIMS. Conclusion: The clinical course of vaccine-associated myocarditis appears favorable, with resolution of the symptoms in all the patients in our cohort.
Assays for the Evaluation of the Immune Response to Marburg and Ebola Sudan Vaccination—Filovirus Animal Nonclinical Group Anti-Marburg Virus Glycoprotein Immunoglobulin G Enzyme-Linked Immunosorbent Assay and a Pseudovirion Neutralization Assay
Authors: Rudge Thomas L. , Machesky Nicholas J. , Sankovich Karen A. , Lemmon Erin E. , Badorrek Christopher S. , Overman Rachel , Niemuth Nancy A. , Anderson Michael S.
Published: August 2022

Since the discovery of the Marburg virus (MARV) in 1967 and Ebola virus (EBOV) in 1976, there have been over 40 reported outbreaks of filovirus disease with case fatality rates greater than 50%. This underscores the need for efficacious vaccines against these highly pathogenic filoviruses. Due to the sporadic and unpredictable nature of filovirus outbreaks, such a vaccine would likely need to be vetted through the U.S. Food and Drug Administration (FDA), following the Animal Rule or similar European Medicines Agency (EMA) regulatory pathway. Under the FDA Animal Rule, vaccine-induced immune responses correlating with survival of non-human primates (NHPs), or another well-characterized animal model, following lethal challenge, will need to be bridged for human immune response distributions in clinical trials. A correlate of protection has not yet been identified for the filovirus disease, but antibodies, specifically anti-glycoprotein (GP) antibodies, are believed to be critical in providing protection against the filovirus disease following vaccination and are thus a strong candidate for a correlate of protection. Thus, species-neutral methods capable of the detection and bridging of these antibody immune responses, such as methods to quantify anti-GP immunoglobulin G (IgG)-binding antibodies and neutralizing antibodies, are needed. Reported here is the development and qualification of two Filovirus Animal Nonclinical Group (FANG) anti-GP IgG Enzyme-Linked Immunosorbent Assays (ELISAs) to quantify anti-MARV and anti-Sudan virus (SUDV) IgG antibodies in human and NHP serum samples, as well as the development of pseudovirion neutralization assays (PsVNAs) to quantify MARV- and SUDV-neutralizing antibodies in human and NHP serum samples.
Poly(hydrophobic Amino Acids) and Liposomes for Delivery of Vaccine against Group A Streptococcus
Authors: Azuar Armira , Madge Harrison Y. R. , Boer Jennifer C. , Gonzalez Cruz Jazmina L. , Wang Jingwen , Khalil Zeinab G. , Deceneux Cyril , Goodchild Georgia , Yang Jieru , Koirala Prashamsa , Hussein Waleed M. , Capon Robert J. , Plebanski Magdalena , Toth Istvan , Skwarczynski Mariusz
Published: August 2022

Adjuvants and delivery systems are essential components of vaccines to increase immunogenicity against target antigens, particularly for peptide epitopes (poor immunogens). Emulsions, nanoparticles, and liposomes are commonly used as a delivery system for peptide-based vaccines. A Poly(hydrophobic amino acids) delivery system was previously conjugated to Group A Streptococcus (GAS)-derived peptide epitopes, allowing the conjugates to self-assemble into nanoparticles with self adjuvanting ability. Their hydrophobic amino acid tail also serves as an anchoring moiety for the peptide epitope, enabling it to be integrated into the liposome bilayer, to further boost the immunological responses. Polyleucine-based conjugates were anchored to cationic liposomes using the film hydration method and administered to mice subcutaneously. The polyleucine-peptide conjugate, its liposomal formulation, and simple liposomal encapsulation of GAS peptide epitope induced mucosal (saliva IgG) and systemic (serum IgG, IgG1 and IgG2c) immunity in mice. Polyleucine acted as a potent liposome anchoring portion, which stimulated the production of highly opsonic antibodies. The absence of polyleucine in the liposomal formulation (encapsulated GAS peptide) induced high levels of antibody titers, but with poor opsonic ability against GAS bacteria. However, the liposomal formulation of the conjugated vaccine was no more effective than conjugates alone self-assembled into nanoparticles.
Discussion of the Trust in Vaccination against COVID-19
Authors: Fan Jiangbo , Wang Xi , Du Shuai , Mao Ayan , Du Haiping , Qiu Wuqi
Published: August 2022

The COVID-19 pandemic has introduced serious challenges to global public health security, and the benefits of vaccination via public health interventions have been recognized as significant. Vaccination is an effective means of preventing and controlling the spread of COVID-19. However, trust is a major factor that influences vaccine hesitancy; thus, the distrust of vaccination has hindered the popularization of COVID-19 vaccines. This paper aims to discuss the main problems and the role of trust in the vaccination against COVID-19 to effectively promote and implement policy to promote the acceptance of COVID-19 vaccines.
Lymphedema of the Arm after COVID-19 Vaccination in a Patient with Hidden Breast Cancer and Paraneoplastic Dermatomyositis
Authors: Aimo Cristina , Mariotti Elena Biancamaria , Corrà Alberto , Quintarelli Lavinia , Bianchi Beatrice , Verdelli Alice , Ruffo di Calabria Valentina , Caproni Marzia
Published: August 2022

The pandemic outbreak of Coronavirus Disease 2019 (COVID-19) led to the development of mRNA vaccines. With the extensive vaccination campaign performed worldwide, many adverse reactions to these drugs have been reported in the literature. Although most of them are mild and self-limiting, they may sometimes cause psychological stress and require efforts to make a differential diagnosis with other conditions. This is the case of lymphadenopathies and lymphedema in patients with a history of cancer. Herein we present a case of lymphedema of the arm developed ten days after a VAXZEVRIA COVID-19 vaccine shot in a patient who had concomitant signs and symptoms compatible with a diagnosis of dermatomyositis. It was later classified as paraneoplastic as instrumental investigation revealed a breast carcinoma contralateral to the site of vaccine injection. With this report we ponder an adverse reaction to COVID-19 vaccination with the aim of bringing new data for clinicians who face similar clinical presentations, particularly controversial for radiologists and oncologists.
Perceptions of Parents towards COVID-19 Vaccination in Children, Aseer Region, Southwestern Saudi Arabia
Authors: Shati Ayed A. , Al-Qahtani Saleh M. , Alsabaani Abdullah A. , Mahmood Syed E. , Alqahtani Youssef A. , AlQahtani Khalid M. , Aldarami Mohammed S. , AlAmri Fahad D. , Alqahtani Abdulrahman Saad , AlHadi Abdulrahman M. , Ahmad Ausaf , Riaz Fatima A.
Published: August 2022

Vaccines are an important part of the COVID-19 pandemic response plan. This cross-sectional study aims to assess the attitude and perception levels of parents toward COVID-19 vaccines for children aged 0–18 years in the Aseer region of Saudi Arabia. Data were analyzed using SPSS version 16.0. Out of a total of 1463 parents, 30.6% assumed that COVID-19 vaccination may be more dangerous for children than adults. Nearly 36.5% parents don’t have any concern about children’s vaccination. About 12.8% of children have not received the vaccination, 55% of parents have some sort of hesitation and 32.2% of parents did not hesitate before vaccinating their children against COVID-19. Only 15.4% of parents expect that the COVID-19 vaccine affects their child’s genes. About 23.4% parents strongly agreed and 35.1% agreed about the importance of getting their children vaccinated. About 22.1% of parents strongly agreed and 33.3% agreed regarding their willingness to get their children vaccinated to prevent Coronavirus disease. More than 80% of parents recommended rushing to receive the COVID-19 vaccine. Health professionals and policymakers should implement and support strategies to ensure children are vaccinated for COVID-19. They also need to educate parents and families regarding childhood vaccination.
Association of COVID-19 Vaccines ChAdOx1-S and BNT162b2 with Circulating Levels of Coagulation Factors and Antithrombin
Authors: Hasan Amal , Arefanian Hossein , Channanath Arshad Mohamed , AlKhairi Irina , Cherian Preethi , Devarajan Sriraman , Thanaraj Thangavel Alphonse , Abu-Farha Mohamed , Abubaker Jehad , Al-Mulla Fahd
Published: August 2022

Background: Severe coronavirus disease 2019 (COVID-19) is associated with increased risk of thrombosis and thromboembolism. Exposure to COVID-19 vaccines is also associated with immune thrombotic thrombocytopenia, ischemic stroke, intracerebral haemorrhage, and cerebral venous thrombosis, and it is linked with systemic activation of coagulation. Methods: We assess the circulating levels of coagulation factors (factors XI, XII, XIII, and prothrombin) and antithrombin in individuals who completed two doses of either ChAdOx1-S or BNT162b2 COVID-19 vaccine, within the timeframe of two months, who had no previous history of COVID-19. Results: Elevated levels of factors XI, XII, XIII, prothrombin, and antithrombin were seen compared to unvaccinated controls. Levels of coagulation factors, antithrombin, and prothrombin to antithrombin ratio were higher with BNT162b2 compared to ChAdOx1-S vaccine. Conclusions: The clinical significance of such coagulation homeostasis disruption remains to be elucidated but it is worthy of global scientific follow-up effort.
Attitudes towards Vaccines, Intent to Vaccinate and the Relationship with COVID-19 Vaccination Rates in Individuals with Schizophrenia
Authors: Raffard Stéphane , Bayard Sophie , Eisenblaetter Margot , Attal Jérôme , Andrieu Christelle , Chereau Isabelle , Fond Guillaume , Leignier Sylvain , Mallet Jasmina , Tattard Philippe , Urbach Mathieu , Misdrahi David , Laraki Yasmine , Capdevielle Delphine
Published: August 2022

Schizophrenia patients are at high risk of developing severe COVID-19 outcomes but recent evidence suggests that they are under-vaccinated. This study explored the role of potential attitudinal barriers by comparing schizophrenia patients with participants from the general population regarding COVID-19 vaccination rates, general attitudes towards vaccines, and willingness to take a COVID-19 vaccine. We conducted a cross-sectional study between April 2021 and October 2021. A total of 100 people with schizophrenia and 72 nonclinical controls were recruited. In our study, individuals with schizophrenia were under-vaccinated, despite similar general attitudes towards vaccination and higher willingness to be vaccinated against COVID-19 compared to nonclinical participants. In patients, negative attitudes toward vaccines were related to higher levels of negative psychotic symptoms and higher levels of paranoid ideation. As a whole, participants with more negative attitudes towards vaccines were less likely to be vaccinated against COVID-19 and had lower levels of trust in institutions. Vaccine hesitancy does not appear to be a major barrier for COVID-19 vaccine uptake amongst people with schizophrenia. This study suggests that disparities in COVID-19 vaccination rates in schizophrenia do not seem related to attitudinal but rather structural barriers.
COVID-19 Vaccination Intent, Barriers and Facilitators in Healthcare Workers: Insights from a Cross-Sectional Study on 2500 Employees at LMU University Hospital in Munich, Germany
Authors: Zhelyazkova Ana , Kim Selina , Klein Matthias , Prueckner Stephan , Horster Sophia , Kressirer Philipp , Choukér Alexander , Coenen Michaela , Adorjan Kristina
Published: August 2022

Considering the role of healthcare workers (HCW) in promoting vaccine uptake and previously recorded hesitancy among HCW, we aim to examine the COVID-19 vaccination intent and status of HCW through a cross-sectional anonymous online survey at LMU University Hospital in Munich. Data collection was informed by the Health Belief Model (HBM) and focused on vaccination intent, status and on potential factors affecting the decision-making process. In total, 2555 employees completed the questionnaire. Our data showed that an approving attitude towards recommended vaccines and having received an influenza vaccine in the previous winter were strongly associated with COVID-19 vaccination intent. Further, a positive COVID-19 vaccination status was associated with a higher likelihood of approving the extension of the validity of non-pharmaceutical interventions at the workplace. Our HBM-analysis demonstrated strong associations between the perceived benefits and barriers and COVID-19 vaccination intent. Unchanged or low perceived susceptibility and severity were associated with refusal or indecisiveness. Our findings highlight the factors associated with the decision regarding a COVID-19 vaccine and indicate a pattern-like behavior in the acceptance of novel vaccines by HCW. These insights can help inform the communication aims of vaccination campaigns among HCW within similar organizational contexts or in future outbreaks.
Association between Adverse Reactions to the First and Second Doses of COVID-19 Vaccine
Authors: Goda Ken , Kenzaka Tsuneaki , Yahata Shinsuke , Okayama Masanobu , Nishisaki Hogara
Published: August 2022

This study investigated the frequency of adverse reactions to COVID-19 vaccines in Japan and the impact of first-dose adverse reactions on second-dose adverse reactions. Individuals who received an mRNA COVID-19 vaccine at our center in March or April 2021 were included. Data were collected using questionnaires. The main factors were age (<40, 40–59, and >60 years), sex, underlying disease, and first-dose adverse reaction. The primary outcomes were incidence of local and systemic adverse reactions (ARs) attributable to the vaccine. Logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Among 671 participants, 90% experienced local or systemic ARs. An AR to the first dose was associated with a significantly increased risk of an AR to the second dose (OR: 49.63, 95% CI: 21.96–112.16). ARs were less common among men than among women (OR: 0.36, 95% CI: 0.17–0.76). Local ARs were less common among those aged 60 years or older (OR: 0.35, 95% CI: 0.18–0.66), whereas systemic ARs were more common among those aged under 40 years. Information on ARs to the first dose is important for healthcare providers and recipients when making vaccination decisions.
Severity Analysis of Large-Truck Wrong-Way Driving Crashes in the State of Florida
Authors: Anam Salwa , Azimi Ghazaleh , Rahimi Alireza , Jin Xia
Published: August 2022

Wrong-way driving (WWD) crashes lead to severe injuries and fatalities, especially when a large truck is involved. This study investigates the factors associated with crash-injury severity in large-truck WWD crashes in Florida. Various driver, roadway, weather, and traffic characteristics were explored as explanatory variables through a random parameter ordered logit model. The study also accounted for heterogeneity by identifying random parameters in the model and introducing interaction effects as potential sources of such heterogeneity. The findings indicate that not using a seatbelt, driving under the influence of drugs, and a driving speed of 50–74 mph were more likely to result in fatal crashes. On the contrary, female drivers, private roadways, and sideswipe collisions showed negative impacts on crash-injury severity. The model identified two random parameters, including a speed of 25–49 mph and early-morning crashes. The interaction effects showed that when driving at a speed of 25–49 mph, young drivers (under 20 years old) and middle-aged drivers (36–50 years old) were the sources of heterogeneity, decreasing crash-injury severity. Understanding the contributing factors of large-truck WWD crashes can help policymakers develop safety countermeasures to reduce the associated injury severity and improve truck safety.
Hormonal Homologies between Canine Mammary Cancer and Human Breast Cancer in a Series of Cases
Authors: de Andrés Paloma Jimena , Cáceres Sara , Illera Juan Carlos , Crespo Belén , Silván Gema , Queiroga Felisbina Luisa , Illera Maria José , Pérez-Alenza Maria Dolores , Peña Laura
Published: August 2022

The validity of spontaneous canine mammary cancer (CMC) as a natural model for the study of human breast cancer (HBC) from a hormonal point of view has never been thoroughly investigated. In this study, we analyzed the immunohistochemical expression of aromatase (Arom) and steroid receptors [estrogen receptor α (ER α), estrogen receptor β (ER β), progesterone receptor (PR) and androgen receptor (AR)] and intratumor steroid hormone levels of 17β-estradiol (E2), estrone sulfate (SO4E1), progesterone (P4), androstenedione (A4), dehydroepiandrosterone (DHEA), and testosterone (T) in 78 samples of mammary cancer—51 human breast cancer (HBC) and 27 canine mammary cancer (CMC)—and corresponding controls. Frequency of tumors expressing Arom, ERβ, PR, and AR was similar in both species, whereas ERα+ tumors were less frequent in the canine species. There was a closer similarity between premenopausal HBC and CMC. In HBC and CMC, all hormones assayed were increased in tumors compared to control samples. Intratumor androgen levels were similar in the two species, although levels of progesterone and estrogens were higher in the HBC samples than the CMC samples. Statistical associations among Arom, receptors, and hormones analyzed suggest that the major hormonal influence in both species is estrogenic through the ER, being the α isoform predominant in the human samples. Our findings further support CMC as a spontaneous model for the study of HBC, especially premenopausal HBC, although several differences, such as the more prevalent ERα immunoexpression and higher intratumor levels of estrogens and P4 in HBC, should be taken into account in comparative hormonal studies.
Canine Fecal Microbiota Transplantation: Current Application and Possible Mechanisms
Authors: Tuniyazi Maimaiti , Hu Xiaoyu , Fu Yunhe , Zhang Naisheng
Published: August 2022

Fecal microbiota transplantation (FMT) is an emerging therapeutic option for a variety of diseases, and is characterized as the transfer of fecal microorganisms from a healthy donor into the intestinal tract of a diseased recipient. In human clinics, FMT has been used for treating diseases for decades, with promising results. In recent years, veterinary specialists adapted FMT in canine patients; however, compared to humans, canine FMT is more inclined towards research purposes than practical applications in most cases, due to safety concerns. Therefore, in order to facilitate the application of fecal transplant therapy in dogs, in this paper, we review recent applications of FMT in canine clinical treatments, as well as possible mechanisms that are involved in the process of the therapeutic effect of FMT. More research is needed to explore more effective and safer approaches for conducting FMT in dogs.
Tolerability of Atovaquone—Proguanil Application in Common Buzzard Nestlings
Authors: Wiegmann Anja , Rinaud Tony , Ottensmann Meinolf , Krüger Oliver , Springer Andrea , Legler Marko , Fehr Michael , Strube Christina , Chakarov Nayden
Published: August 2022

Differences in drug tolerability among vertebrate groups and species can create substantial challenges for wildlife and ex situ conservation programmes. Knowledge of tolerance in the use of new drugs is, therefore, important to avoid severe toxicity in species, which are both commonly admitted in veterinary clinics and are of conservation concern. Antimalarial drugs have been developed for use in human medicine, but treatment with different agents has also long been used in avian medicine, as haemosporidian infections play a major role in many avian species. This study investigates the effects of the application of atovaquone–proguanil (Malarone®, GlaxoSmithKline) in common buzzards (Buteo buteo). The potential effects of treatment on body condition, growth rate, and chemical blood parameters of nestlings were assessed. All individuals survived the treatment, and no effects on body condition, growth rate, and chemical blood parameters were observed. Our results suggest the tolerability of Malarone® in common buzzards at a single dose of on average 11 mg/kg body weight. For its safe use, we recommend further studies to determine pharmacokinetics in different avian species as well as to assess the effects of repeated treatment.
Evaluation of a Novel Precordial Lead System for the Electrocardiographic Diagnosis of Right Ventricular Enlargement in Dogs
Authors: Grosso Giovanni , Vezzosi Tommaso , Pergamo Cesara Sofia , Bini Martina , Patata Valentina , Domenech Oriol , Tognetti Rosalba
Published: August 2022

The purpose of this study was to evaluate the reliability of precordial leads for the detection of right ventricular enlargement (RVE) in dogs. This was a prospective observational study. The RVE was defined by echocardiography. The amplitude (mV) of the Q, R, and S waves, the R/S ratio, and the mean electrical axis (MEA) of the QRS complex were assessed on the 12-lead ECG. The ROC curve and the Youden index yielded the best cutoffs for RVE detection. An area under the curve (AUC) > 0.7 defined suitable diagnostic accuracy. A total of 84 dogs, 27 with RVE and 57 healthy controls, were enrolled. Q wave amplitude in aVR (cutoff > 0.10 mV; AUC = 0.727), R/S ratio in V4 (cutoff < 1.15; AUC = 0.842), R/S ratio in V5 (cutoff < 1.95; AUC = 0.839) and S wave amplitude in V6 (cutoff > 0.70 mV; AUC = 0.703) showed suitable diagnostic accuracy in detecting RVE. Among dogs with RVE, only 9/27 (33%) presented a right shift of MEA. Differently, 19/27 (70%) showed at least one of the identified precordial lead criteria. Assessment of the R/S ratio in V4 and V5 and S wave amplitude in V6 increases the diagnostic accuracy of ECG in distinguishing between dogs with RVE and healthy dogs.
Clinical Case of Feline Leishmaniosis: Therapeutic Approach and Long-Term Follow-Up
Authors: Napoli Ettore , De Benedetto Giovanni , Fazio Cristina , La Russa Francesco , Gaglio Gabriella , Brianti Emanuele
Published: August 2022

The response to allopurinol treatment and survival time of a case of feline leishmaniosis in a FIV co-infected cat is herein reported. In May 2019, a 13-year-old neutered European Shorthair male was referred due to weight loss and exfoliative dermatitis. Lymphadenomegaly and splenomegaly were detected upon clinical inspection, while the presence of several Leishmania infantum amastigotes were detected on splenic and lymphonodal fine needle aspiration (FNA). Allopurinol (10 mg/kg PO q 12 h) was administered. After two months, the cat’s clinical symptoms disappeared, and the owners decided to interrupt the therapy. In February, two reddish nodular fleshy neoformations appeared in both eyes, and amastigotes of Leishmania were detected by cytology on conjunctival swabs. Allopurinol treatment was re-started at the same rate; the ocular lesions regressed in two weeks, and the owner again decided to interrupt the therapy. In July, the patient had a new relapse, but the owner, tired of continuous relapses, refused further treatment of the disease. The cat’s health condition continued to worsen: in October 2021, the ocular lesions appeared again, and in November the patient died. This case underlines the effectiveness of allopurinol and highlights how interruption of treatment frequently leads to relapse, impairing the animal’s health condition and prognosis.
Comparison between Some Phenotypic and Genotypic Methods for Assessment of Antimicrobial Resistance Trend of Bovine Mastitis Staphylococcus aureus Isolates from Bulgaria
Authors: Rusenova Nikolina , Vasilev Nasko , Rusenov Anton , Milanova Aneliya , Sirakov Ivo
Published: August 2022

The aim of this study was to assess the resistance of bovine mastitis S. aureus isolates from farms in Bulgaria to different classes of chemotherapeutic drugs by comparison of some phenotypic and genotypic methods by means of Cohen’s kappa statistics. The study comprised 546 milk samples from subclinical and clinical mastitis at 14 farms from 9 districts in the country. A total of 92 Staphylococcus aureus strains were isolated from tested samples and identified by nuc PCR. The results demonstrated high levels of resistance to sulfadimethoxine (87%), followed by resistance to penicillin (33.7%), erythromycin (13%), streptomycin (8.7%), tetracycline (6.5%) and gentamicin (1.1%). The comparison of both phenotypic tests with respect to 9 antimicrobials revealed strong agreement with kappa coefficient 0.836. An almost complete agreement was evidenced between phenotypic resistance to penicillin and blaZ gene presence, to methicillin with mecA gene, to tetracycline with tet genes, but the agreement between erythromycin resistance and erm genes presence was moderate. This study was the first to demonstrate discrepancy between the behaviour to cefoxitin in the disk diffusion test and oxacillin in the MIC test for an isolate shown to carry the mecA gene in the subsequent genetic analysis. Considering the detected discrepancies for some of isolates, an integral evaluation through phenotypic and molecular methods for monitoring of antimicrobial resistance of Staphylococcus aureus is recommended.
Dumping of Dredge Spoil in the Pelagic Habitat: Focus on Trophic Status, Phytoplankton Diversity Responses and Generation of Blooms
Authors: Varkitzi Ioanna , Pavlidou Alexandra , Pantazi Maria , Rousselaki Eleni , Hatiris Georgios-Angelos , Gratsia Eirini , Kapsimalis Vasilios , Pagou Kalliopi
Published: August 2022

This study presents the impacts of dredge spoil dumping in the pelagic habitat during a 27-month monitoring survey in eastern Mediterranean coastal waters (Saronikos Gulf, Aegean Sea), with a focus on changes in trophic status and eutrophication levels, phytoplankton diversity and bloom dynamics. A number of environmental parameters and phytoplankton metrics were significantly influenced by the dumping operations, specifically phytoplankton diversity indices (number of species, Diatoms:Dinoflagellates ratio) and total abundance, Chlorophyll-a, light transmission, dissolved oxygen and inorganic nutrients, N:P ratio, and the Eutrophication Ιndex (a metric for trophic status assessment). Phosphates started to increase after the first year of dumping operations, shifting the N:P ratio to values lower than 10. A similarity cluster analysis highlighted that the phytoplankton community structure during the pre-dumping and the early-dumping period was clearly discriminated from the period during and after the dumping operations. A clear shift with an increase in the Diatoms:Dinoflagellates ratio was observed immediately after the initiation of dumping operations, which maximized in the dumping site after two years of operations. Diatoms dominated the phytoplankton communities, reaching ~ 95% relative abundance in the dumping site. High biomass producers or potentially toxic diatom species proliferated forming blooms. Pseudo-nitzschia multiseries was the most frequent potentially toxic species. A multivariate analysis (RDA) highlighted that among a suite of phytoplankton metrics plotted against stressors relevant to dumping, the Eutrophication Index, Chlorophyll-a, the diversity index Diatoms:Dinoflagellates ratio and the abundance of the potentially toxic diatom P. multiseries emerged as the most suitable to reflect the responses of phytoplankton communities to dumping. Dredge spoil dumping at sea poses pressures to ecosystem components addressed by the European Marine Strategy Framework Directive (MSFD) monitoring programs. In such a context, this study further supports the role of phytoplankton diversity and blooms as sensitive monitoring elements for the environmental status assessment and dumping management in coastal waters.
Modeling the Effects of Climate Change and Land Use/Land Cover Change on Sediment Yield in a Large Reservoir Basin in the East Asian Monsoonal Region
Authors: Li Huiyun , Yu Chuanguan , Qin Boqiang , Li Yuan , Jin Junliang , Luo Liancong , Wu Zhixu , Shi Kun , Zhu Guangwei
Published: August 2022

This research addresses the separate and combined impacts of changes in climate and land use/land cover on the hydrological processes and sediment yield in the Xin’anjiang Reservoir Basin (XRB) in the southeast of China by using the soil and water assessment tool (SWAT) hydrological model in combination with the downscaled general circulation model (GCM) projection outputs. The SWAT model was run under a variety of prescribed scenarios including three climate changes, two land use changes, and three combined changes for the future period (2068–2100). The uncertainty and attribution of the sediment yield variations to the climate and land use/land cover changes at the monthly and annual scale were analyzed. The responses of the sediment yield to changes in climate and land use/land cover were considered. The results showed that all scenarios of climate changes, land use/land cover alterations, and combined changes projected an increase in sediment yield in the basin. Under three representative concentration pathways (RCP), climate change significantly increased the annual sediment yield (by 41.03–54.88%), and deforestation may also increase the annual sediment yield (by 1.1–1.2%) in the future. The comprehensive influence of changes in climate and land use/land cover on sediment yield was 97.33–98.05% (attributed to climate change) and 1.95–2.67% (attributed to land use/land cover change) at the annual scale, respectively. This means that during the 2068–2100 period, climate change will exert a much larger influence on the sediment yield than land use/land cover alteration in XRB if the future land use/land cover remains unchanged after 2015. Moreover, climate change impacts alone on the spatial distribution of sediment yield alterations are projected consistently with those of changes in the precipitation and water yield. At the intra-annual scale, the mean monthly transported sediment exhibits a significant increase in March–May, but a slight decrease in June–August in the future. Therefore, the adaptation to climate change and land use/land cover change should be considered when planning and managing water environmental resources of the reservoirs and catchments.
Determinants of Spatial Variability of Ice Thickness in Lakes in High Mountains of the Temperate Zone—The Case of the Tatra Mountains
Authors: Solarski Maksymilian , Rzetala Mariusz
Published: August 2022

Vertical and horizontal variation in the ice cover of mountain lakes in the temperate climate zone has not been thoroughly studied. The study concerned ice phenomena in four lakes located in the Tatra National Park in the Tatra Mountains (the Czarny Staw Gąsienicowy, Czarny Staw pod Rysami, Morskie Oko, and Smreczyński Staw). The research, which was conducted in the 2018/2019 winter season, included an analysis of variability in atmospheric conditions, an analysis of presence of ice phenomena on satellite images, field work (measurements of ice layer and of snow and slush layer thickness were conducted at a total of 151 sites), and statistical analyses. It was determined that negative air temperature was just one factor among those that determined the maximum thickness of the ice forming on lakes in high mountains. It was found that in addition to ambient thermal conditions, a major factor affecting the magnitude of variation in lake ice thickness was the thickness of the snow overlying the ice and its spatial variability. Thicker ice cover tended to form in areas where a thick layer of snow was deposited. The decisive factor that contributed to a significant variation in ice thickness between lakes was the uneven accretion of snow ice from above. The maximum ice thickness values modeled using Stefan’s formula were significantly underestimated (accounting for 38–61% of the ice thickness measured) relative to the highest ice thickness values found empirically at the end of the winter season. Study results fill a gap in our knowledge and methodology related to vertical and horizontal variation in the ice cover of mountain lakes; they also have significant applications, indicating the risk of winter use of water bodies with different ice cover structure, thickness, and extent.
Developing Indicators of Nutrient Pollution in Streams Using 16S rRNA Gene Metabarcoding of Periphyton-Associated Bacteria
Authors: Pilgrim Erik M. , Smucker Nathan J. , Wu Huiyun , Martinson John , Nietch Christopher T. , Molina Marirosa , Darling John A. , Johnson Brent R.
Published: August 2022

Indicators based on nutrient-biota relationships in streams can inform water quality restoration and protection programs. Bacterial assemblages could be particularly useful indicators of nutrient effects because they are species-rich, important contributors to ecosystem processes in streams, and responsive to rapidly changing conditions. Here, we sampled 25 streams weekly (12–14 times each) and used 16S rRNA gene metabarcoding of periphyton-associated bacteria to quantify the effects of total phosphorus (TP) and total nitrogen (TN). Threshold indicator taxa analysis identified assemblage-level changes and amplicon sequence variants (ASVs) that increased or decreased with increasing TP and TN concentrations (i.e., low P, high P, low N, and high N ASVs). Boosted regression trees confirmed that relative abundances of gene sequence reads for these four indicator groups were associated with nutrient concentrations. Gradient forest analysis complemented these results by using multiple predictors and random forest models for each ASV to identify portions of TP and TN gradients at which the greatest changes in assemblage structure occurred. Synthesized statistical results showed bacterial assemblage structure began changing at 24 µg TP/L with the greatest changes occurring from 110 to 195 µg/L. Changes in the bacterial assemblages associated with TN gradually occurred from 275 to 855 µg/L. Taxonomic and phylogenetic analyses showed that low nutrient ASVs were commonly Firmicutes, Verrucomicrobiota, Flavobacteriales, and Caulobacterales, Pseudomonadales, and Rhodobacterales of Proteobacteria, whereas other groups, such as Chitinophagales of Bacteroidota, and Burkholderiales, Rhizobiales, Sphingomonadales, and Steroidobacterales of Proteobacteria comprised the high nutrient ASVs. Overall, the responses of bacterial ASV indicators in this study highlight the utility of metabarcoding periphyton-associated bacteria for quantifying biotic responses to nutrient inputs in streams.
Seasonal Differences in the Hydrochemical Characteristics of Karst Wetlands and the Associated Mechanisms in Huixian, China
Authors: Ba Junjie , Dan Yong , Luo Fei , Tang Chunlei , Peng Cong
Published: August 2022

The present study aimed to identify the seasonal changes in the hydrochemical characteristics of the Huixian karst wetland and the underlying mechanism. Conventional ions and isotopes of 130 groundwater samples collected during the wet and dry seasons were analyzed. The hydrochemical characteristics of groundwater in the Huixian karst wetland were clarified using mathematical statistics and hydrochemical methods, including Durov diagrams, ionic ratios, Gibbs diagrams, and H and O isotopes. The hydrochemical evolution and sources of major ions in the Huixian karst wetland were also investigated. The results showed that cations and anions in groundwater in the study area were dominated by Ca2+ and HCO3−, respectively, sourced mainly from calcite weathering. The ions of some groundwater samples were regulated by weathering of dolomite, dolomitic limestone, and pyrite, resulting in relatively high concentrations of Mg2+ and SO42−. K+, Na+, SO42−, NO3−, and Cl− in groundwater originated from precipitation, Na+ and Cl− originated from domestic sewage, K+ was related to the application of potassium fertilizer in agriculture, and NO3− mainly originated from chemical fertilizer. Groundwater ions were primarily controlled by rock weathering, followed by evaporative concentration. The sources of major ions were dependent on the dissolution and precipitation of carbonate rock, as well as the chemical weathering of silicate rock and evaporite. Samples from the various water sources were scattered on both sides of the local meteoric water line (δD = 3.13δ18O − 13.9), which indicated isotopic composition was affected by evaporation and precipitation.
Feasibility Assessment of the Application of Groundwater Remediation Techniques in Rural Areas: A Case Study of Rural Areas in the Soutpansberg Region, Limpopo Province, South Africa
Authors: Lalumbe Lindelani , Oberholster Paul Johan , Kanyerere Thokozani
Published: August 2022

Groundwater contribution towards human health and livelihood depends on the contaminants level in groundwater. Many people in rural communities are being exposed to waterborne diseases resulting from drinking untreated contaminated groundwater. This study argues that the lack of implementation of available groundwater remediation methods and associated high costs are exposing rural communities to health risks. This study assessed 22 years of groundwater quality data from 12 boreholes and 2 springs to understand the contaminants level in the Soutpansberg region. A feasibility assessment of the application and design of a sustainable groundwater remediation technique was carried out based on individual- and community-based groundwater remediation types. The assessment considered groundwater management, cost and risk of theft and damage to infrastructure model for rural settings. This study determined that groundwater was not suitable for drinking purposes in some parts due to high concentration levels of NO3− and F−. The feasibility assessment indicated that community-based groundwater remediation schemes are more sustainable in rural areas when compared to individual household remediation. In this study, it is recommended that groundwater remediation plans must be included in any proposed water supply or drought intervention project in rural communities.