bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Implicit Followership Antecedents on the New Generation of Individuals
Authors: Lu Kunxiu , Zhang Wei , Zhang Xiaoyan , Feng Junshu , Li Ying
Published: July 2022

This study used the Predisposition Proposition Theory of implicit followership to determine the effect of a proactive personality and core self-evaluation on the implicit followership of different schemas. Intertemporal survey data for one month from 452 university graduates were collected to evaluate that core self-evaluation significantly and positively affects positive implicit followership and significantly and negatively affects negative followership. However, the effect of proactive personality on implicit followership is not significant. The results of data analysis support the interpretation of propensity propositions in the study of personality traits. This study also determines the theoretical significance and practical application value.
Impact of Managerial Trustworthy Behavior on Employee Engagement: Mediating Role of Perceived Insider Status
Authors: Liu Defeng , Bakari Haroon , Niaz Maharukh , Zhang Qianxiao , Shah Imran Ahmed
Published: July 2022

This study examines the impact of managerial trustworthy behavior on employees’ engagement and the mediating role of perceived insider status. This study has adopted an exploratory research design and positivist philosophy. The data are collected from 205 healthcare staff working in public sector hospitals in Pakistan through survey questionnaires, using a convenience sampling technique. Partial Least Square Structural equation modeling is used to analyze the data and test hypotheses. Results indicate that managerial trustworthy behavior relates positively to employee engagement. Perceived insider status mediates the relationship between managerial trustworthy behavior and employee engagement. The major limitation of this study is its cross-sectional design which limits the casualty. However, this study offers important insights regarding trust-building, engagement, and inclusion in the health sector. This study highlights the importance of trust-building among managers and employees. Managers who instill more trust in employees will garner more positive behavior. This study offers fresh insights into managers’ trustworthy behavior toward employees’ engagement and the employees’ perceived insider status within their organizations.
Exploring the Multidisciplinary Factors Affecting Sports Talent Identification
Authors: Xiang Changqing , Tengku Kamalden Tengku Fadilah , Liu Hejian , Ismail Normala
Published: July 2022

Talent is one of the most significant factors to promote the development of sports undertakings. The present study aimed to explore the factors affecting the identification of sports talents in China's physical education curriculum. Based on the literature review, this study puts forward a model to examine the influencing factors of sports talent identification in China's physical education curriculum using structural equation modeling and uses the structural equation modeling and factor analysis method to verify the hypothesis combined with the results of 310 effective questionnaires. The article summarizes influencing factors from four aspects, namely, physical, psychological, coach, and environmental factors. On the basis of relevant literature, the hypothesis model was established by structural equation modeling. The results show that the main factors affecting the identification of sports talents in the physical education curriculum are personal physical quality performance, psychological quality, coach's knowledge, and the identification policies of schools to sports talents. The conclusion of this study can provide guidance for the reform of the physical education curriculum, the growth of sports talents, and the development of sports talents in China.
Clustering Performances in Elite Basketball Matches According to the Anthropometric Features of the Line-ups Based on Big Data Technology
Authors: Xu Xiao , Zhang Mingxin , Yi Qing
Published: July 2022

The aims of this study were: 1) to conduct a descriptive analysis of the anthropometric features of the line-ups of strong teams (top 16) in the 2019 FIBA Basketball World Cup; 2) to group the line-ups mentioned above into different clusters based on their average height, weight, and body mass index (BMI); and 3) to explore the performance variables that discriminate between various line-up clusters. The play-by-play statistics were collected from 104 team objects in 67 games and 525 line-ups were analyzed using two-step cluster and discriminant analysis. Line-ups were classified into four groups: low average height and weight with middle BMI (LowH–LowW–MiddleBMI); high average height and low average weight with low BMI (HighH–LowW–LowBMI); low average height and high average weight with high BMI (LowH–HighW–HighBMI); high average height and weight with middle BMI (HighH–HighW–MiddleBMI). The results of the discriminant analysis demonstrated that LowH–LowW–MiddleBMI line-ups had the least time played and the lowest offensive rating, but the best offensive rebounds, turnovers, and fastest game pace performance; HighH–LowW–LowBMI line-ups demonstrated the best defensive rating but performed poorly with a low value of assists and a high value of turnovers; the LowH–HighW–HighBMI group achieved the best time played statistics but had the lowest number of free throws made; the HighH–HighW–MiddleBMI group had a higher number of assists and a higher offensive rating and 2-point field goal performance, while also achieving the lowest number of offensive rebounds and ball possessions. These results provide novel insights for coaches and performance analysts to better understand the technical characteristics of different line-ups in elite basketball competitions.
A Predictive Model of Risk Factors for Conversion From Major Depressive Disorder to Bipolar Disorder Based on Clinical Characteristics and Circadian Rhythm Gene Polymorphisms
Authors: Xu Zhi , Chen Lei , Hu Yunyun , Shen Tian , Chen Zimu , Tan Tingting , Gao Chenjie , Chen Suzhen , Chen Wenji , Chen Bingwei , Yuan Yonggui , Zhang Zhijun
Published: July 2022

Background

Bipolar disorder (BD) is easy to be misdiagnosed as major depressive disorder (MDD), which may contribute to a delay in treatment and affect prognosis. Circadian rhythm dysfunction is significantly associated with conversion from MDD to BD. So far, there has been no study that has revealed a relationship between circadian rhythm gene polymorphism and MDD-to-BD conversion. Furthermore, the prediction of MDD-to-BD conversion has not been made by integrating multidimensional data. The study combined clinical and genetic factors to establish a predictive model through machine learning (ML) for MDD-to-BD conversion.

Method

By following up for 5 years, 70 patients with MDD and 68 patients with BD were included in this study at last. Single nucleotide polymorphisms (SNPs) of the circadian rhythm genes were selected for detection. The R software was used to operate feature screening and establish a predictive model. The predictive model was established by logistic regression, which was performed by four evaluation methods.

Results

It was found that age of onset was a risk factor for MDD-to-BD conversion. The younger the age of onset, the higher the risk of BD. Furthermore, suicide attempts and the number of hospitalizations were associated with MDD-to-BD conversion. Eleven circadian rhythm gene polymorphisms were associated with MDD-to-BD conversion by feature screening. These factors were used to establish two models, and 4 evaluation methods proved that the model with clinical characteristics and SNPs had the better predictive ability.

Conclusion

The risk factors for MDD-to-BD conversion have been found, and a predictive model has been established, with a specific guiding significance for clinical diagnosis.
Involuntary Psychiatric Admission: Arbitrary Deprivation of Liberty or a Human Right?
Authors: Tamai Sergio
Published: July 2022

In 2008 Brazil ratified The United Nations (UN) Convention on the Rights of Persons with Disabilities (CRPD) an international legal instrument specifically tailored to stipulate the rights of persons with disabilities and include those with serious mental disorders. United Nations Committee set up to monitor the implementation of the Convention (CRPD Committee) lead to an insistence that involuntary detention and treatment of people with mental health (or “psychosocial”) disabilities are prohibited. There is a debate about this topic that poses an impossibility of involuntary psychiatric admission in hospital.
Affective Empathy, Theory of Mind and Social Functioning in Patients With Focal Epilepsy
Authors: Metternich Birgitta , Wagner Kathrin , Geiger Maximilian J. , Schulze-Bonhage Andreas , Hirsch Martin , Schönenberg Michael
Published: July 2022

Objective

Social cognition comprises basic and more complex functions, such as theory of mind (ToM) and affective empathy. Although everyday social interactions may be impaired if such higher-order social cognitive functions are compromised, associations between social functioning and social cognition in people with focal epilepsy (PWFE) are still poorly understood. We used a novel, naturalistic approach to investigate ToM in PWFE by applying the Movie for the Assessment of Social Cognition (MASC). Furthermore, we studied affective empathy, the relationship between social cognitive parameters and measures of social functioning, as well as between epilepsy focus and ToM.

Methods

Thirty patients with either temporal (TLE) or frontal lobe epilepsy (FLE) were compared to 29 healthy control subjects (HC). In addition to the MASC, we applied questionnaire measures assessing empathy and everyday social functioning.

Results

PWFE, especially with FLE, performed significantly worse than HC on the MASC. Perceived social integration and social activities, but not affective empathy, were reduced in PWFE. Regression analyses revealed associations between perceived social integration, clinical group status, affective empathy and ToM.

Conclusion

PWFE displayed ToM deficits during a naturalistic task, whereas affective empathy was unimpaired. FLE may be associated with especially compromised ToM performance. Social cognition and social functioning appear to be interrelated in PWFE, whose self-perceived levels of social integration and social activities are lower than those of HC. More research into the association between social cognition and social functioning in PWFE is needed, in order to develop tailored intervention programs for these patients.
Analysis of Multiple Health Risky Behaviours and Associated Disease Outcomes Using Scottish Linked Hospitalisation Data
Authors: Olajide Damilola , Eberth Barbara , Ludbrook Anne
Published: July 2022

Background

Disease incidence and premature deaths tend to be influenced by multiple health risky behaviours, including smoking, excessive alcohol consumption and unhealthy diet. Risky behaviours tend not to be independent and may have a multiplicative effect on disease incidence and healthcare cost. Thus, understanding the interrelationship between health behaviours and their effect on health outcomes is crucial in designing behavioural intervention programmes.

Objective

To examine the interrelationship between health risky behaviours and associated disease outcomes amongst Scottish adults.

Methods

We use hospitalisation episode data from the Scottish Morbidity Records, (SMR), that have been administratively linked to Scottish Health Surveys (SHeS) respondents with target disease defined by relevant ICD9 and 10 codes. We apply a recursive multivariate probit model to jointly estimate the health risky behaviours and disease incidence to adequately control for unobserved heterogeneity. The model is estimated separately by gender.

Results

Modelling health risk behaviours and disease incidence equations independently rather than jointly may be misleading. We find a clear socioeconomic gradient predicting health risky behaviours and the results differ by gender. Specifically, smoking appears to be a key driver of other health risky behaviours. Current smokers are more likely to be drinking above the recommended limit, physically inactive, and eating inadequate diet.

Conclusions

Interventions targeting current smokers to quit could spillover to other behaviours by reducing excessive drinking, improve physical activity and adequate diet. Thus, improvements in one behaviour may increase the likelihood of adopting other healthier lifestyle behaviours.
Case Report: Mycobacterium senegalense Infection After Cholecystectomy
Authors: Zhou Huiling , Yang Hong , Gong Fengling , Zhou Shaolong , Yang Yifeng , Liu Haidan , Liu Jijia
Published: July 2022

Background

Mycobacterium senegalense is a non-tuberculous mycobacterium and is found everywhere in the environment. However, M. senegalense infection in human is extremely rare, especially in immunocompetent individuals. It is difficult to detect M. senegalense infection because its symptoms are non-specific, and routine diagnostic tests are less sensitive. It is also resistant to commonly used antibiotics. Here, we report the first case of M. senegalense infection after laparoscopic cholecystectomy in China.

Case Presentation

A 55-year-old man was admitted because of repeated infections at multiple incision sites for more than 1 year. Although routine diagnostic test results were negative, metagenomic next-generation sequencing (mNGS) identified DNA sequences of M. senegalense in tissue samples from incision sites. The presence of M. senegalense was further confirmed by polymerase chain reaction and capillary electrophoresis. After 60 days of quadruple therapy with clarithromycin, moxifloxacin, rifampicin, and oxycycline, the patient's wound healed.

Conclusion

We believe the case findings contribute to the limited amount of knowledge about M. senegalense infection and raises awareness that this infection can result in poor wound healing, even in an immunocompetent host. Owing to a lack of early, precise diagnosis, it is difficult to treat M. senegalense infections. Based on our findings, mNGS is a sensitive diagnostic test for M. senegalense infections.
Satisfaction With Community Health Education Among Residents in China: Results From a Structural Equation Model
Authors: Tong Yuting , Wang Haipeng , Zhu Kangming , Zhao Hanhan , Qi Yangrui , Guan Jiahui , Ma Yuanyuan , Li Qiyu , Sun Xinying , Wu Yibo
Published: July 2022

Background

Although community health education has drawn lots of attention from the public, evidence on resident satisfaction is still sparse. This study aims to explore the relationships among five dimensions (perceived quality, perceived value, public expectation, public trust, and public satisfaction) of satisfaction with community health education among Chinese residents.

Methods

We constructed a theoretical public satisfaction model for community health education based on the American Customer Satisfaction Index (ACSI) model. There are five dimensions in the theoretical model, including public expectation, perceived quality, perceived value, public satisfaction, and public trust. We recruited 474 respondents from a quota sampling based on gender and age, and collected information on five dimensions of satisfaction with community health education. The relationships of the five dimensions were examined using structural equation model.

Results

The mean scores of public expectation, perceived quality, perceived value, public satisfaction, and public trust for the participants were 11.44 (total 15), 123.89 (total 170), 14.18 (total 20), 10.19 (total 15), and 15.61 (total 20), respectively. We obtained a structural equation model with a good fitting degree. There was a direct effect of perceived quality on perceived value (γ = 0.85, P < 0.01), public trust (γ = 0.81, P < 0.01) and public satisfaction (γ = 0.58, P < 0.01), and a direct effect of public expectation on public satisfaction (γ = 0.36, P < 0.01) and perceived value (γ = 0.25, P < 0.01).

Conclusions

We provide a good tool to measure public satisfaction with community health education, which can be potentially used to measure public satisfaction and improve the effectiveness of health education.
Food Promotion and Children's Health: Considering Best Practices for Teaching and Evaluating Media Literacy on Food Marketing
Authors: Elliott Charlene , Truman Emily , Nelson Michelle R. , Scheibe Cyndy , Hudders Liselot , De Jans Steffi , Brisson-Boivin Kara , McAleese Samantha , Johnson Matthew , Walker Lauren , Ellison Kirsten
Published: July 2022

Food marketing to children is ubiquitous and persuasive. It primarily promotes foods of poor nutritional quality, influences children's food preferences and habits, and is a factor in childhood obesity. Given that food marketing relentlessly targets children in traditional/digital media and the built environment, children need critical media literacy skills that build their understanding of food marketing's persuasive effects. However, little research connects media literacy with food marketing and health, including effective strategies for teaching and evaluating such programming for children. This perspective presents the outcomes of a stakeholder meeting on best practices in teaching and evaluation on media literacy and food marketing to children. Strategies for promoting critical thinking (teaching content, teaching practices, teaching supports, and parent/caregiver involvement), and strategies for measuring critical thinking (program effectiveness and broader long-term impacts) were identified. These include, among other things, the need to capture the range of marketing formats and current food promotion trends, to include inquiry-based and co-creation activities, and to support ongoing media literacy development. Overall, these strategies suggest useful criteria for media literacy programming related to food marketing, and highlight the importance of media literacy for giving children the skills to navigate a complex food environment.
Awareness, Utilization and Health Outcomes of National Essential Public Health Service Among Migrants in China
Authors: Xu Xinpeng , Zhang Qinglong , You Hua , Wu Qifeng
Published: July 2022

Background

The national essential public health service (NEPHS) has been in operation for more than a decade. Numerous studies examined the utilization of NEPHS by migrants and the factors that influence it, but few examined the effect of NEPHS awareness and utilization on the health of inhabitants, particularly migrants. The purpose of this study is to ascertain the level of awareness and utilization of NEPHS, as well as to examine their health-improving effects on migrants.

Methods

Based on the data from the 2017 China Migrants Dynamic Survey, linear probability model, ordered logit model and the propensity score matching methods were employed to investigate impact of awareness and utilization of NEPHS on the health among Chinese migrants. Mediating effect model were used to identify the mechanism of the impact of NEPHS on health.

Results

The findings indicated that migrants' awareness and utilization of NEPHS are still insufficient. After adjusting for other factors, the study discovered that increased awareness and use of NEPHS had a beneficial influence on migrants' self-rated health. Further heterogeneity analysis revealed significant disparities in the health consequences of NEPHS awareness and utilization across subgroups. The effect of increased awareness and usage of NEPHS on health is stronger for middle-aged and elderly people, women, and low-educated migrants with urban household registration. The estimated results of the mediating effect model supported the mechanism that increased NEPHS awareness among the floating population could encourage its utilization and further improve the floating population's health.

Conclusions

Given that migrants' NEPHS utilization is still low and that NEPHS utilization has a positive effect on health, some targeted strategies, such as a variety of new media communication methods, health education related to occupational disease and tuberculosis prevention, and targeted NEPHS projects for specific groups, such as men, young and middle-aged groups, those with a high level of education, and rural migrants, should be conducted to improve the health of migrants.
Tobacco Consumption and Mental Health in the Canary Islands: A Quantitative Analysis
Authors: Nieto-González Imanol L. , Rodríguez-Donate M. Carolina , Guirao-Pérez Ginés
Published: July 2022

Although the detrimental health effects of tobacco, there has been scant research into determining comprehensive profiles to characterize individuals with a higher risk of smoking. This paper identifies such profiles and probabilistically quantifies the effects of certain traits on the decision to smoke in the Canary Islands. This characterization is approached from a broad and novel point of view for the region studied, given that the analysis not only studies sociodemographic characteristics, lifestyle and health, but also incorporates mental health. The results show that suffering from some type of psychopathology leads to a higher probability of tobacco consumption. Moreover, just as the promotion of a healthy lifestyle has a direct impact on tobacco consumption, the implementation of public policies that reduce the risks of suffering from a mental illness could also reduce the prevalence of smoking in the region and contribute to the design of more effective prevention strategies.
Auxin-Inducible Degron System Reveals Temporal-Spatial Roles of HSF-1 and Its Transcriptional Program in Lifespan Assurance
Authors: Morphis Allison C. , Edwards Stacey L. , Erdenebat Purevsuren , Kumar Lalit , Li Jian
Published: July 2022

HSF-1 is a key regulator of cellular proteotoxic stress response and is required for animal lifespan. In C. elegans, HSF-1 mediated heat shock response (HSR) declines sharply on the first day of adulthood, and HSF-1 was proposed to function primarily during larval stages for lifespan assurance based on studies using RNAi. The tissue requirement for HSF-1 in lifespan, however, is not well understood. Using the auxin-inducible degron (AID) system, we manage to uncouple the roles of HSF-1 in development and longevity. In wild-type animals, we find HSF-1 is required during the whole self-reproductive period for lifespan. This period is extended in long-lived animals that have arrested germline stem cells (GSC) or reduced insulin/IGF-1 signaling (IIS). While depletion of HSF-1 from any major somatic tissues during development results in severe defects, HSF-1 primarily functions in the intestine and likely neural system of adults to support lifespan. Finally, by combining AID and genome-wide transcriptional analyses, we find HSF-1 directly activates the transcription of constitutively-expressed chaperone and co-chaperone genes among others in early adulthood, which underlies its roles in longevity assurance.
A Unifying Framework for Reinforcement Learning and Planning
Authors: Moerland Thomas M. , Broekens Joost , Plaat Aske , Jonker Catholijn M.
Published: July 2022

Sequential decision making, commonly formalized as optimization of a Markov Decision Process, is a key challenge in artificial intelligence. Two successful approaches to MDP optimization arereinforcement learningandplanning, which both largely have their own research communities. However, if both research fields solve the same problem, then we might be able to disentangle the common factors in their solution approaches. Therefore, this paper presents a unifying algorithmic framework for reinforcement learning and planning (FRAP), which identifies underlying dimensions on which MDP planning and learning algorithms have to decide. At the end of the paper, we compare a variety of well-known planning, model-free and model-based RL algorithms along these dimensions. Altogether, the framework may help provide deeper insight in the algorithmic design space of planning and reinforcement learning.
Active Transportation, the Ultimate Low Carbon Way to Travel—A Review of International Research and Education
Authors: Allen Heather , Nolmark Henrik
Published: July 2022

Active transport1 is a relatively new term but one that has been gaining support over the past decade as an alternative to “non-motorized transport” (NMT). Walking and cycling are the main transport modes within the active transport cluster. This paper summarizes the current state of international research and knowledge on active transport in urban and peri-urban areas in high-, middle- and low-income countries. It also explores current research gaps and potential “future looking” research areas. The information was collected in early 2020 and is based on a rapid literature review, a 15-question Internet survey, semi-structured interviews, and a peer-review process with key actors and experts in active transport. Respondents were professionally active in a broad range of areas directly or indirectly connected to the topic. A total of 112 cleaned responses2 were obtained from high, middle- and low-income countries. The main findings show that there is a high level of scholarship in the subject, with a greater breadth of research in high income countries. There has been an increase in research attention since 1990. Both are widely covered by work on road safety aspects. A key difference can be seen between cycling and walking and their positioning as transport modes. More papers on cycling are published in transport-related journals, while walking is better covered in health-related journals. Based on the identified research gaps found in this review, suggestions are put forward for further possible research areas of interest on active transport in the post COVID recovery.
Digital Mapping of Agricultural Soil Organic Carbon Using Soil Forming Factors: A Review of Current Efforts at the Regional and National Scales
Authors: Xia Yushu , McSweeney Kevin , Wander Michelle M.
Published: July 2022

To explore how well large spatial scale digital soil mapping can contribute to efforts to monitor soil organic carbon (SOC) stocks and changes, we reviewed regional and national studies quantifying SOC within lands dominated by agriculture using SCORPAN approaches that rely on soil (S), climate (C), organisms (O), relief (R), parent material (P), age (A), and space (N) covariates representing soil forming factors. After identifying 79 regional (> 10,000 km2) and national studies that attempted to estimate SOC, we evaluated model performances with reference to soil sampling depth, number of predictors, grid-distance, and spatial extent. SCORPAN covariates were then investigated in terms of their frequency of use and data sources. Lastly, we used 67 studies encompassing a variety of spatial scales to determine which covariates most influenced SOC in agricultural lands using a subjective ranking system. Topography (used in 94% of the cases), climate (87%), and organisms (86%) covariates that were the most frequently used SCORPAN predictors, aligned with the factors (precipitation, temperature, elevation, slope, vegetation indices, and land use) currently identified to be most influential for model estimate at the large spatial extent. Models generally succeeded in estimating SOC with fits represented by R2 with a median value of 0.47 but, performance varied widely (R2 between 0.02 and 0.86) among studies. Predictive success declined significantly with increased soil sampling depth (p < 0.001) and spatial extent (p < 0.001) due to increased variability. While studies have extensively drawn on large-scale surveys and remote sensing databases to estimate environmental covariates, the absence of soils data needed to understand the influence of management or temporal change limits our ability to make useful inferences about changes in SOC stocks at this scale. This review suggests digital soil mapping efforts can be improved through greater use of data representing soil type and parent material and consideration of spatio-temporal dynamics of SOC occurring within different depths and land use or management systems.
Self-determination in Physical Exercise Predicts Creative Personality of College Students: The Moderating Role of Positive Affect
Authors: Chen Shaoqing , Wang Qing , Wang Xinya , Huang Liying , Zhang Dongdong , Shi Baoguo
Published: July 2022

Although previous studies indicated that intrinsic motivation and positive affect were important for creativity and proved the benefits of physical exercise for creativity, little is known about underlying this relationship between self-determination in physical exercise and creative personality among college students. Based on prior studies and theoretical models, the current study investigated the relationship between self-determination in physical exercise and creativity and the moderating role of positive affect in the relationship between self-determination in physical exercise and creative personality. This model was analyzed with 1,201 Chinese college students (Mean age = 20.10 years, SD = 0.93). Participants filled out the physical exercise self-determination scale, the Williams creativity assessment packet, and the satisfaction with life scale. The results indicated that self-determination in physical exercise was significantly positively correlated with the creative personality of college students, including risk-taking, curiosity, challenge, and imagination. Moreover, self-determination in physical exercise could significantly positively predict the creative personality of college students. Moderation analysis further showed that the relationship between self-determination in physical exercise and creative personality was robust for college students with low levels of positive affect. These findings suggest the importance of cultivating students' creative ability through improving exercise autonomy, especially for those college students with a low level of positive affect.
A Mechanistic Cellular Atlas of the Rheumatic Joint
Authors: Zerrouk Naouel , Aghakhani Sahar , Singh Vidisha , Augé Franck , Niarakis Anna
Published: July 2022

Rheumatoid Arthritis (RA) is an autoimmune disease of unknown aetiology involving complex interactions between environmental and genetic factors. Its pathogenesis is suspected to arise from intricate interplays between signalling, gene regulation and metabolism, leading to synovial inflammation, bone erosion and cartilage destruction in the patients’ joints. In addition, the resident synoviocytes of macrophage and fibroblast types can interact with innate and adaptive immune cells and contribute to the disease’s debilitating symptoms. Therefore, a detailed, mechanistic mapping of the molecular pathways and cellular crosstalks is essential to understand the complex biological processes and different disease manifestations. In this regard, we present the RA-Atlas, an SBGN-standardized, interactive, manually curated representation of existing knowledge related to the onset and progression of RA. This state-of-the-art RA-Atlas includes an updated version of the global RA-map covering relevant metabolic pathways and cell-specific molecular interaction maps for CD4+ Th1 cells, fibroblasts, and M1 and M2 macrophages. The molecular interaction maps were built using information extracted from published literature and pathway databases and enriched using omic data. The RA-Atlas is freely accessible on the webserver MINERVA (https://ramap.uni.lu/minerva/), allowing easy navigation using semantic zoom, cell-specific or experimental data overlay, gene set enrichment analysis, pathway export or drug query.
Case Report: Clinical and Serological Hallmarks of Cytokine Release Syndrome in a Canine B Cell Lymphoma Patient Treated With Autologous CAR-T Cells
Authors: Atherton Matthew J. , Rotolo Antonia , Haran Kumudhini P. , Mason Nicola J.
Published: July 2022

Background

Chimeric antigen receptor-T (CAR-T) cells have transformed the treatment of human B cell malignancies. With the advent of CAR-T therapy, specific and in some cases severe toxicities have been documented with cytokine release syndrome (CRS) being the most frequently reported. As dogs develop tumors spontaneously and in an immunocompetent setting, they provide a unique translational opportunity to further investigate the activity and toxicities associated with CAR-T therapy. Although various adoptive cellular therapy (ACT) trials have been documented and several more are ongoing in canine oncology, CRS has not been comprehensively described in canine cancer patients.

Case Presentation

Here we present the clinical and serologic changes in a dog treated with autologous CAR-T for relapsed B cell lymphoma that presented with lethargy and fever 3 days following CAR-T. Multiplexed serum cytokine profiling revealed increases in key cytokines implicated in human CRS including IL-6, MCP-1, IFNγ and IL-10 at or shortly after peak CAR-T levels in vivo.

Conclusion

The observations noted in this case report are consistent with CRS development following CAR-T therapy in a canine patient. The dog represents a compelling model to study the pathophysiology of CRS and pre-clinically screen novel therapeutics to prevent and treat this life-threatening condition in the setting of a complex and naturally evolved immune system.
Assessing the Relationship Between Emotional States of Dogs and Their Human Handlers, Using Simultaneous Behavioral and Cardiac Measures
Authors: Grigg Emma K. , Liu Serene , Dempsey Denise G. , Wong Kylee , Bain Melissa , Sollers John J. , Haddock Rani , Kogan Lori R. , Barnhard Jennifer A. , Tringali Ashley A. , Thigpen Abigail P. , Hart Lynette A.
Published: July 2022

Negative stress due to human handling has been reported for a number of domestic animals, including dogs. Many companion dogs display significant stress during routine care in the veterinary clinic, risking injury to staff and potentially compromising the quality of care that these dogs receive. On the other hand, positive interactions with humans can have a beneficial effect on dogs, particularly in stressful situations such as animal shelters. Research has shown that dogs can detect human emotions through visual, auditory, and chemical channels, and that dogs will exhibit emotional contagion, particularly with familiar humans. This study investigated relationships between emotional states of dogs and unfamiliar human handlers, using simultaneous measures of cardiac activity and behavior, during two sessions of three consecutive routine handling sets. Measures of cardiac activity included mean heart rate (HRmean), and two measures of heart rate variability (HRV): the root mean square of successive differences between normal heartbeats (RMSSD); and the high frequency absolute power component of HRV, log transformed (HFlog). We also assessed human handlers' emotional state during handling sessions following an intervention designed to reduce stress, compared with sessions conducted on a different day and following a control activity. Polar H10 cardiac sensors were used to simultaneously record cardiac activity for both canine and human participants, and behavioral data were collected via digital video. The strongest influence on the dogs' stress levels in our study was found to be increasing familiarity with the setting and the handler; HRmeanand SI decreased, and HRV (as RMSSD) increased, significantly from the first to the third handling set. Canine HRV (as HFlog) was also highest in set 3, although the difference was not statistically significant. There were no strong patterns found in the human cardiac data across handling set, session, or by pre-handling activity. We did not find consistent support for emotional contagion between the dogs and their handlers in this study, perhaps due to the brief time that the dogs spent with the handlers. Recommendations for application to dog handling, and limitations of our methods, are described.
FATP1 Exerts Variable Effects on Adipogenic Differentiation and Proliferation in Cells Derived From Muscle and Adipose Tissue
Authors: Huang Jieping , Guo Duo , Zhu Ruirui , Feng Ye , Li Ruirui , Yang Xintong , Shi Deshun
Published: July 2022

In livestock, intramuscular adipose tissue is highly valued whereas adipose tissue in other depots is considered as waste. Thus, genetic factors that favor fat deposition in intramuscular compartments over that in other adipose depots are highly desirable in meat-producing animals. Fatty acid transport 1 (FATP1) has been demonstrated to promote cellular fatty acid uptake and metabolism; however, whether it also influences cellular lipid accumulation remains unclear. In the present study, we investigated the effects of FATP1 on the differentiation and proliferation of adipocytes in five types of cells derived from muscle and adipose tissue and estimated the effects of FATP1 on intramuscular fat (IMF) deposition. We showed that FATP1 is mainly expressed in heart and muscle tissue in buffaloes as well as cells undergoing adipogenic differentiation. Importantly, we found that FATP1 promoted the adipogenic differentiation of muscle-derived cells (buffalo myocytes and intramuscular preadipocytes and mouse C2C12 cells) but did not affect, or even inhibited, that of adipose-derived cells (buffalo subcutaneous preadipocytes and mouse 3T3-L1 cells, respectively). Correspondingly, our results further indicated that FATP1 promotes IMF deposition in mice in vivo. Meanwhile, FATP1 was found to enhance the proliferative activity of all the assessed cells, except murine 3T3-L1 cells. These results provide new insights into the potential effects of FATP1 on IMF deposition, especially regarding its positive effects on meat quality in buffaloes and other livestock.
Enteric Escherichia coli O157:H7 in Cattle, and the Use of Mice as a Model to Elucidate Key Aspects of the Host-Pathogen-Microbiota Interaction: A Review
Authors: Lange Maximo E. , Uwiera Richard R. E. , Inglis G. Douglas
Published: July 2022

Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is responsible for foodborne disease outbreaks, typically associated with the consumption of undercooked foods contaminated with cattle manure containing the bacterium. At present, effective mitigations do not exist. Many of the factors regulating enteric colonization by E. coli O157:H7 in cattle, and how cattle respond to the bacterium are unknown. In this regard, intestinal colonization locations, shedding patterns, interactions with the enteric microbiota, and host immune responses to infection are current knowledge gaps. As disturbances to host homeostasis are believed to play an important role in the enteric survival of the bacterium, it is important to consider the potential importance of stress during cattle production. Husbandry logistics, cost, and the high genetic, physiological, and microbial heterogeneity in cattle has greatly hampered the ability of researchers to elucidate key aspects of the host-pathogen-microbiota interaction. Although mice have not been extensively used as a cattle model, the utilization of murine models has the potential to identify mechanisms to facilitate hypothesis formulation and efficacy testing in cattle. Murine models have been effectively used to mechanistically examine colonization of the intestine, host responses to infection, and to interactively ascertain how host physiological status (e.g., due to physiological stress) and the enteric microbiota influences colonization and disease. In addition to reviewing the relevant literature on intestinal colonization and pathogenesis, including existing knowledge gaps, the review provides information on how murine models can be used to elucidate mechanisms toward the development of rationale-based mitigations for E. coli O157:H7 in cattle.
Dietary 25-Hydroxyvitamin D3 Supplementation Modulates Intestinal Cytokines in Young Broiler Chickens
Authors: Abascal-Ponciano Gerardo A. , Leiva Samuel F. , Flees Joshua J. , Avila Luis P. , Starkey Jessica D. , Starkey Charles W.
Published: July 2022

Vitamin D signaling is important for intestinal homeostasis. An increase in vitamin D receptors in immune cells can modulate cell phenotype and cytokine secretion. Cytokines regulate both pro- (interleukin 17; IL-17) and anti-inflammatory (IL-10) responses triggered by external stimuli. Inflammation in intestinal tissues can disrupt the structure and the remodeling of epithelial tight junction complexes, thus, compromising the protective barrier. The objective of the study was to determine the impact of dietary supplementation with 25-hydroxycholecalciferol (25OHD3), a hydroxylated metabolite of vitamin D, on intestinal cytokine abundance and epithelial barrier integrity over time in broilers. A randomized complete block design experiment was conducted to evaluate the effect of dietary 25OHD3 inclusion on relative protein expression of the cytokines, IL-17 and IL-10, and tight junction proteins, Zona Occludens 1 (ZO-1), and Claudin-1 (CLD-1), in broiler chicken duodenum and ileum from 3 to 21 days post-hatch. On day 0, male chicks (n = 168) were randomly assigned to raised floor pens. Experimental corn–soybean meal-based treatments were as follows: (1) a common starter diet containing 5,000 IU of D3 per kg of feed (VITD3) and (2) a common starter diet containing 2,240 IU of D3 + 2,760 IU of 25OHD3 per kg of feed (25OHD3) fed from days 0 to 21. On days 3, 6, 9, 12, 15, 18, and 21, 12 birds per treatment were euthanized to collect tissue samples for quantitative, multiplex, and fluorescent Western blot analysis. Target proteins were quantified using Image Quant TL 8.1 and expressed relative to total protein. Feeding 25OHD3 post-hatch decreased ileal IL-10 (anti-inflammatory) protein expression in 21-day-old broilers compared with VITD3 only (P = 0.0190). Broilers fed only VITD3 post-hatch had greater IL-17 (pro-inflammatory) protein expression in the ileum at 18 and 21 days-of-age (P = 0.0412) than those that fed 25OHD3. Dietary inclusion of 25OHD3 lowered the abundance of key inflammatory cytokines in the ileum of young broilers.
Systemic Treatment of Breast Cancer. 1st Central-Eastern European Professional Consensus Statement on Breast Cancer
Authors: Rubovszky Gábor , Kocsis Judit , Boér Katalin , Chilingirova Nataliya , Dank Magdolna , Kahán Zsuzsanna , Kaidarova Dilyara , Kövér Erika , Krakovská Bibiana Vertáková , Máhr Károly , Mriňáková Bela , Pikó Béla , Božović-Spasojević Ivana , Horváth Zsolt
Published: July 2022

This text is based on the recommendations accepted by the 4th Hungarian Consensus Conference on Breast Cancer, modified based on the international consultation and conference within the frames of the Central-Eastern European Academy of Oncology. The professional guideline primarily reflects the resolutions and recommendations of the current ESMO, NCCN and ABC5, as well as that of the St. Gallen Consensus Conference statements. The recommendations cover classical prognostic factors and certain multigene tests, which play an important role in therapeutic decision-making. From a didactic point of view, the text first addresses early and then locally advanced breast cancer, followed by locoregionally recurrent and metastatic breast cancer. Within these, we discuss each group according to the available therapeutic options. At the end of the recommendations, we summarize the criteria for treatment in certain rare clinical situations.
Eficacia de una Intervención Psicológica Breve en Línea Durante el Confinamiento por COVID-19
Authors: González Brignardelo Marcela Paz , Olea Tejero Teresa , Carrasco Miguel Ángel , Saúl Luis Ángel , Sierra Purificación
Published: July 2022

El objetivo de este estudio fue evaluar la eficacia de la intervención psicológica en línea ofrecida a los usuarios por el Servicio de Psicología Aplicada durante el período de alerta sanitaria decretado por la COVID-19. Adicionalmente, se cuantificó la satisfacción y eficacia percibida por el usuario, después de dicha intervención.
Se incluyeron 60 adultos (70.4%, mujeres) con una edad promedio de 41.49 años. Los instrumentos que se utilizaron pre y post-intervención fueron las versiones abreviadas   del Listado de Síntomas (SA-45), la medida de resultados clínicos (CORE-OM), el Inventario de Afrontamiento (COPE-28) y la Escala de Resiliencia de Connor-Davidson (CD-RISK). El Cuestionario de Satisfacción del Cliente (CSQ-8) y la Percepción percibida de la eficacia de la intervención fueron cumplimentados después de finalizada la intervención. Este dispositivo fue diseñado con los objetivos de disminuir la sintomatología inicial mediante psicoeducación y ejercicios breves de regulación para potenciar las estrategias positivas de afrontamiento y resiliencia. Los resultados mostraron una disminución significativa (p < .05) de la sintomatología inicial, en todas las dimensiones analizadas y una mejoría de las estrategias de afrontamiento y resiliencia. Además, se observaron puntuaciones globales elevadas en la satisfacción y eficacia percibida por el usuario. En conclusión, nuestros resultados apuntan hacia una elevada eficacia de la intervención breve en línea en situaciones de confinamiento.
Efficacy of acceptance and commitment therapy focused on repetitive negative thinking in fibromyalgia: A randomized multiple-baseline design
Authors: Leidy Estefany López-Palomo  , Juan C. Vargas-Nieto  , Francisco J. Ruiz
Published: July 2022

Repetitive negative thinking (RNT), in the form of worry and rumination, is a factor that can have a negative impact on the quality of life and symptomatology of patients with fibromyalgia (FM). The present study analyzes the efficacy of a brief Acceptance and Commitment Therapy (ACT) protocol focused on reducing RNT in four women diagnosed with FM. A randomized, multiple-baseline design across participants was conducted. Participants completed 4-6 weeks of baseline and subsequently received a 4-session individual intervention. The effect of the intervention was assessed by conducting follow-ups for up to 3 months. All four participants showed clinically significant changes in emotional symptoms as measured by the Depression Anxiety and Stress Scale – 21 (DASS-21¸ S. H. Lovibond y P. F. Lovibond, 1995) and the General Health Questionnaire – 12 (Ruiz et al., 2017a). Likewise, participants showed significant improvements in parameters related to sleep quality and improvements in health-related quality of life. As for process measures, all participants showed clinically significant changes in pathological worry, and three of them also in cognitive fusion. Changes in valued actions were more modest. Effect sizes comparable across designs were very large and statistically significant for DASS-Total (d = 1.51), DASS-Depression (d = 1.83), pathological worry (d = 1.79), and cognitive fusion (d = 1.99). These results suggest that brief RNT-focused ACT interventions hold promise for intervention in patients with FM.