bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Peptide-Based Inhibitors for Microtubule Polymerization in Phytophthora capsici
Authors: Lee Sang-Choon , Kim Sang-Heon , Hoffmeister Rachel A. , Yoon Moon-Young , Kim Sung-Kun
Published: May 2019

The plant disease Phytophthora blight, caused by the oomycete pathogen Phytophthora capsici, is responsible for major economic losses in pepper production. Microtubules have been an attractive target for many antifungal agents as they are involved in key cellular events such as cell proliferation, signaling, and migration in eukaryotic cells. In order to design a novel biocompatible inhibitor, we screened and identified inhibitory peptides against alpha- and beta-tubulin of P. capsici using a phage display method. The identified peptides displayed a higher binding affinity (nanomolar range) and improved specificity toward P. capsici alpha- and beta-tubulin in comparison to Homo sapiens tubulin as evaluated by fluorometric analysis. One peptide demonstrated the high inhibitory effect on microtubule formation with a nanomolar range of IC50 values, which were much lower than a well-known chemical inhibitor—benomyl (IC50 = 500 µM). Based on these results, this peptide can be employed to further develop promising candidates for novel antifungal agents against Phytophthora blight.
Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells
Authors: Facchin Federica , Alviano Francesco , Canaider Silvia , Bianconi Eva , Rossi Martina , Bonsi Laura , Casadei Raffaella , Biava Pier Mario , Ventura Carlo
Published: May 2019

Stem cells undergo senescence both in vivo, contributing to the progressive decline in self-healing mechanisms, and in vitro during prolonged expansion. Here, we show that an early developmental zebrafish embryo extract (ZF1) could act as a modulator of senescence in human mesenchymal stem cells (hMSCs) isolated from both adult tissues, including adipose tissue (hASCs), bone marrow (hBM-MSCs), dental pulp (hDP-MSCs), and a perinatal tissue such as the Wharton’s Jelly (hWJ-MSCs). In all the investigated hMSCs, ZF1 decreased senescence-associated β-galactosidase (SA β-gal) activity and enhanced the transcription of TERT, encoding the catalytic telomerase core. In addition, it was associated, only in hASCs, with a transcriptional induction of BMI1, a pleiotropic repressor of senescence. In hBM-MSCs, hDP-MSCs, and hWJ-MSCs, TERT over-expression was concomitant with a down-regulation of two repressors of TERT, TP53 (p53), and CDKN1A (p21). Furthermore, ZF1 increased the natural ability of hASCs to perform adipogenesis. These results indicate the chance of using ZF1 to modulate stem cell senescence in a source-related manner, to be potentially used as a tool to affect stem cell senescence in vitro. In addition, its anti-senescence action could also set the basis for future in vivo approaches promoting tissue rejuvenation bypassing stem cell transplantation.
Novel Approaches in Ovarian Cancer Research against Heterogeneity, Late Diagnosis, Drug Resistance, and Transcoelomic Metastases
Authors: Erol Anna , Niemira Magdalena , Krętowski Adam Jacek
Published: May 2019

The development of modern technologies has revolutionised science and has had a huge impact on biomedical studies. This review focuses on possible tools that scientists can use to face the challenges of fighting ovarian cancer. Ovarian cancer is the deadliest gynaecologic malignancy and, even after years of study, the mortality has not decreased significantly. In the era of sequencing and personalised and precision medicine, we are now closer than ever to helping patients and physicians in regard to treatment and diagnosis of this disease. This work summarises the newest findings in the development of ovarian cancer research.
Correction: Marcuzzi, E., et al. Chemokines and Chemokine Receptors: Orchestrating Tumor Metastasization. Int. J. Mol. Sci. 2019, 20, 96
Authors: Marcuzzi Elisabetta , Angioni Roberta , Molon Barbara , Calì Bianca
Published: May 2019

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The Key Role of Warm and Cold Ischemia in Uterus Transplantation: A Review
Authors: Tardieu Antoine , Dion Ludivine , Lavoué Vincent , Chazelas Pauline , Marquet Pierre , Piver Pascal , Sallée Camille , Aubard Yves , Barin-Le Guellec Chantal , Favreau Frederic , Gauthier Tristan
Published: May 2019

Introduction: Uterus transplantation (UTx) is a promising treatment for uterine infertility that has resulted in several births since 2014. Ischemia is a key step in organ transplantation because it may lead to changes jeopardizing graft viability. Method: We performed a systematic review of animal and human studies relating to uterine ischemia. Results: We retained 64 studies published since 2000. There were 35 studies in animals, 24 in humans, and five literature reviews. Modest preliminary results in large animals and humans are limited but encouraging. In small animals, pregnancies have been reported to occur after 24 h of cold ischemia (CI). In ewes, uterine contractions have been detected after 24 h of CI. Furthermore, it has been shown in animals that uterine tolerance to CI and to warm ischemia (WI) can be increased by pharmacological products. In women, mean CI time in studies of births from uteri obtained from live donors was between 2 h 47 min and 6 h 20 min from a deceased donor; with only one birth in this case. Muscle contractions have also been demonstrated in myometrial samples from women, after six or more hours of CI. Conclusion: The uterus seems to be able to tolerate a prolonged period of CI, of at least six hours. Studies of the ischemia tolerance of the uterus and ways to improve it are essential for the development of UTx, particularly for procedures using grafts from deceased donors.
Aggressive Exclusion of Scan Flip-Flops from Compression Architecture for Better Coverage and Reduced TDV: A Hybrid Approach
Authors: Shantagiri Pralhadrao V. , Kapur Rohit
Published: May 2019

Scan-based structural testing methods have seen numerous inventions in scan compression techniques to reduce TDV (test data volume) and TAT (test application time). Compression techniques lead to test coverage (TC) loss and test patterns count (TPC) inflation when higher compression ratio is targeted. This happens because of the correlation issues introduced by these techniques. To overcome this issue, we propose a new hybrid scan compression technique, the aggressive exclusion (AE) of scan cells from compression for increasing overall TC and reduce TPC. This is achieved by excluding scan cells which contribute to 12% to 43% of overall care bits from compression architecture, placing them in multiple scan chains with dedicated scan-data-in and scan-data-out ports. The selection of scan cells to be excluded from the compression technique is done based on a detailed analysis of the last 95% of the patterns from a pattern set to reduce correlations. Results show improvements in TC of up to 1.33%, and reductions in TPC of up to 77.13%.
Thermal Characterization of Low-Dimensional Materials by Resistance Thermometers
Authors: Fu Yifeng , Cui Guofeng , Jeppson Kjell
Published: May 2019

The design, fabrication, and use of a hotspot-producing and temperature-sensing resistance thermometer for evaluating the thermal properties of low-dimensional materials are described in this paper. The materials that are characterized include one-dimensional (1D) carbon nanotubes, and two-dimensional (2D) graphene and boron nitride films. The excellent thermal performance of these materials shows great potential for cooling electronic devices and systems such as in three-dimensional (3D) integrated chip-stacks, power amplifiers, and light-emitting diodes. The thermometers are designed to be serpentine-shaped platinum resistors serving both as hotspots and temperature sensors. By using these thermometers, the thermal performance of the abovementioned emerging low-dimensional materials was evaluated with high accuracy.
Metamorphic Integration of GaInAsSb Material on GaAs Substrates for Light Emitting Device Applications
Authors: Lu Qi , Marshall Andrew , Krier Anthony
Published: May 2019

The GaInAsSb material has been conventionally grown on lattice-matched GaSb substrates. In this work, we transplanted this material onto the GaAs substrates in molecular beam epitaxy (MBE). The threading dislocations (TDs) originating from the large lattice mismatch were efficiently suppressed by a novel metamorphic buffer layer design, which included the interfacial misfit (IMF) arrays at the GaSb/GaAs interface and strained GaInSb/GaSb multi-quantum wells (MQWs) acting as dislocation filtering layers (DFLs). Cross-sectional transmission electron microscopy (TEM) images revealed that a large part of the dislocations was bonded on the GaAs/GaSb interface due to the IMF arrays, and the four repetitions of the DFL regions can block most of the remaining threading dislocations. Etch pit density (EPD) measurements indicated that the dislocation density in the GaInAsSb material on top of the buffer layer was reduced to the order of 106 /cm2, which was among the lowest for this compound material grown on GaAs. The light emitting diodes (LEDs) based on the GaInAsSb P-N structures on GaAs exhibited strong electro-luminescence (EL) in the 2.0–2.5 µm range. The successful metamorphic growth of GaInAsSb on GaAs with low dislocation densities paved the way for the integration of various GaInAsSb based light emitting devices on the more cost-effective GaAs platform.
Modelling of Coupled Shrinkage and Creep in Multiphase Formulations for Hardening Concrete
Authors: Gamnitzer Peter , Brugger Andreas , Drexel Martin , Hofstetter Günter
Published: May 2019

The durability and serviceability of concrete structures is influenced by both the early-age behavior of concrete as well as its long-term response in terms of shrinkage and creep. Hygro-thermo-chemo-mechanical models, as they are used in the present publication, offer the possibility to consistently model the behavior of concrete from the first hours to several years. However, shortcomings of the formulation based on effective stress, which is usually employed in such multiphase models, were identified. As a remedy, two alternative formulations with a different coupling of shrinkage and creep are proposed in the present publication. Both assume viscous flow creep to be driven by total stress instead of effective stress, while viscoelastic creep is driven either by total or effective stress. Therefore, in contrast to the formulation based on effective stress, they predict a limit value for shrinkage as observed in long-term drying shrinkage tests. Shrinkage parameters for the new formulations are calibrated based on drying shrinkage data obtained from thin slices. The calibration process is straightforward for the new formulations since they decouple shrinkage and viscous flow creep. The different formulations are compared using results from shrinkage tests on sealed and unsealed cylindrical specimens. Shrinkage strain predictions are significantly improved by the new formulations.
Effect of Crystallinity of Polyethylene with Different Densities on Breakdown Strength and Conductance Property
Authors: Li Dawei , Zhou Liwei , Wang Xuan , He Lijuan , Yang Xiong
Published: May 2019

In order to study the effects of the crystallinity of polyethylene with different densities on breakdown strength and conductance properties, this paper mainly tests the X-ray diffraction (XRD), different scanning calorimeter (DSC), direct current (DC) breakdown and conductance properties of low-density polyethylene (LDPE), linear low density polyethylene (LLDPE), medium density polyethylene (MDPE), and high-density polyethylene (HDPE), and further analyzes the experimental results separately. The results show that an increase in the density of polyethylene leads to the continuous improvement of crystallinity, and an increase in crystallinity causes a significant decrease in the conduction current at the same field strength. The field strength corresponding to the two turning points in the conductance characteristic curve increases simultaneously.
Structural Properties and Damage Detection Capability of Carbon Nanotube Modified Mortars after Freeze-Thaw
Authors: Alafogianni Panagiota , Tragazikis Ilias , Balaskas Anastasios , Barkoula Nektaria-Marianthi
Published: May 2019

Here we explore the structural properties and damage sensing of cementitious mortars after a freeze-thaw process (F-T) as a function of nano-modification. For this purpose, carbon nanotubes were added at 0.2–0.8 wt.% cement using two different dispersive agents. F-T resulted in reduced fracture energy in nano-modified specimens prepared using superplasticizer as a dispersant while the opposite held true for the surfactant-containing ones. All nano-modified mortars possessed significantly higher fracture energy compared to the plain specimens after F-T (up to 73% improvement). The acoustic emission activity was lower after F-T, while acoustic emission indicators revealed a more tensile mode of fracture in both plain and nano-modified mortars.
Differences in Dry Sliding Wear Behavior between Al–12Si–CuNiMg Alloy and Its Composite Reinforced with Al2O3 Fibers
Authors: Zhang Qing , Gu Jie , Wei Shuo , Qi Ming
Published: May 2019

The dry sliding wear behavior of the Al-12Si-CuNiMg matrix alloy and its composite reinforced with Al2O3 fibers was investigated using a pin-on-disk wear-testing machine. The volume fraction of Al2O3 fibers in the composite was 17 vol.%. Wear tests are conducted under normal loads of 2.5, 5.0, and 7.5 N, and sliding velocities of 0.25, 0.50, and 1.0 m/s. Furthermore, the worn surfaces of the matrix alloy and the composite were examined using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The results showed that the wear resistance of the composite was inferior to that of the matrix alloy, which could be attributed to the high content of reinforcement and casting porosities in the composite. Worn-surface analysis indicates that the dominant wear mechanisms of both materials were abrasive wear and adhesive wear under the present testing conditions.
A Thermal Tactile Sensation Display with Controllable Thermal Conductivity
Authors: Hirai Seiya , Miki Norihisa
Published: May 2019

We demonstrate a thermal tactile sensation display that can present various thermal sensations, namely cold/cool/warm/hot feelings, by varying the effective thermal conductivity of the display. Thermal sensation is one of the major tactile sensations and needs to be further investigated for advanced virtual reality/augmented reality (VR/AR) systems. Conventional thermal sensation displays present hot/cold sensations by changing the temperature of the display surface, whereas the proposed display is the first one that controls its effective thermal conductivity. The device contains an air cavity and liquid metal that have low and high thermal conductivity, respectively. When the liquid metal is introduced to fill up the air cavity, the apparent thermal conductivity of the device increases. This difference in the thermal conductivity leads to the users experiencing different thermal tactile sensations. Using this device, the threshold to discriminate the effective thermal conductivity was experimentally deduced for the first time. This thermal tactile display can be a good platform for further study of thermal tactile sensation.
Detection of Cassava Component in Sweet Potato Noodles by Real-Time Loop-mediated Isothermal Amplification (Real-time LAMP) Method
Authors: Wang Deguo , Wang Yongzhen , Zhu Kai , Shi Lijia , Zhang Meng , Yu Jianghan , Liu Yanhong
Published: May 2019

Sweet potato (Ipomoea batatas) noodles are a traditional Chinese food with a high nutritional value; however, starch adulteration is a big concern. The objective of this study was to develop a reliable method for the rapid detection of cassava (Manihot esculenta) components in sweet potato noodles to protect consumers from commercial adulteration. Five specific Loop-mediated Isothermal Amplification (LAMP) primers targeting the internal transcribed spacer (ITS) of cassava were designed, genomic DNA was extracted, the LAMP reaction system was optimized, and the specificity of the primers was verified with genomic DNA of cassava, Ipomoea batatas, Zea mays, and Solanum tuberosum; the detection limit was determined with a serial dilution of adulterated sweet potato starch with cassava starch, and the real-time LAMP method for the detection of the cassava-derived ingredient in sweet potato noodles was established. The results showed that the real-time LAMP method can accurately and specifically detect the cassava component in sweet potato noodles with a detection limit of 1%. Furthermore, the LAMP assay was validated using commercial sweet potato noodle samples, and results showed that 57.7% of sweet potato noodle products (30/52) from retail markets were adulterated with cassava starch in China. This study provides a promising solution for facilitating the surveillance of the commercial adulteration of sweet potato noodles from retail markets.
Mass Spectrometry-Based Untargeted Metabolomics and α-Glucosidase Inhibitory Activity of Lingzhi (Ganoderma lingzhi) During the Developmental Stages
Authors: Satria Dedi , Tamrakar Sonam , Suhara Hiroto , Kaneko Shuhei , Shimizu Kuniyoshi
Published: May 2019

Lingzhi is a Ganoderma mushroom species which has a wide range of bioactivities. Analysis of the changes in metabolites during the developmental stages of lingzhi is important to understand the underlying mechanism of its biosynthesis, as well as its bioactivity. It may also provide valuable information for the cultivation efficiency of lingzhi. In this study, mass spectrometry based untargeted metabolomics was carried out to analyze the alteration of metabolites during developmental stages of lingzhi. Eight developmental stages were categorized on the basis of morphological changes; starting from mycelium stage to post-mature stage. GC/MS and LC/MS analyses along with multivariate analysis of lingzhi developmental stages were performed. Amino acids, organic acids, sugars, polyols, fatty acids, fatty alcohols, and some small polar metabolites were extracted as marker metabolites from GC/MS analysis, while, lanostane-type triterpenoids were observed in LC/MS analysis of lingzhi. The marker metabolites from untargeted analysis of lingzhi developmental stages were correlated with the α-glucosidase inhibitory activity. Two metabolites, compounds 34 and 35, were identified as potential contributors of the α-glucosidase inhibitory activity. The current result shows that some metabolites are involved in the developmental process and α-glucosidase inhibitory activity of lingzhi.
Bioactive Exopolysaccharides Reveal Camellia oleifera Infected by the Fungus Exobasidium gracile Could Have a Functional Use
Authors: Dong Zhe , Liu Wen , Zhou Dejian , Li Peipei , Wang Teng , Sun Kunlai , Zhao Yuqin , Wang Jie , Wang Bin , Chen Yin
Published: May 2019

Camellia oleifera is an important Chinese commercial crop. Camellia oleifera can display abnormal leaves due to infection by the parasitic fungus Exobasidium gracile. Exobasidium gracile was isolated from infected leaves and used in fermentation, and exopolysaccharides EP0-1 and EP0.5-1 were purified from the fermentation broth. EP0-1 was an alkaline polysaccharide consisting mainly of the linkages α-d-Manp(1→, →2)-α-d-Manp(1→ and →6)-α-d-Manp(1→, →3)-α-d-Glcp(1→ and→4)-α-d-Glcp(1→, terminal β-d-Galf, (1→5)-β-d-Galf, and terminal β-D-GlcN(1→. EP0.5-1 was an acidic galactofuranose-containing polysaccharide. It contained the linkages of α-d-Manp(1→, →2)-α-d-Manp(1→, →6)-α-d-Manp(1→,→2, 6)-α-d-Manp(1→, →4)-α-d-Glcp(1→, and →4)-α-d-GlcUA(1→. Galactofuranose linkages were composed of terminal β-d-Galf, (1→6)-β-d-Galf and (1→2)-β-d-Galf. Exobasidium gracile exopolysaccharides displayed significant immunoregulatory activity by activating macrophages. This research indicates that infected leaves from Camellia oleifera including the exopolysaccharides produced by the parasitic fungus Exobasidium gracile by are worth further investigation as a functional product.
Assessment of the Antioxidant/Hypolipidemic Relationship of Sideritis hyssopifolia in an Experimental Animal Model
Authors: Coto Esther , Fernandez Nelida , Garcia Juan Jose , Diez M. Jose , Sahagun Ana Maria , Sierra Matilde
Published: May 2019

Many publications have described the potential cardioprotective action of different medicinal plants, relating this effect with blood lipid levels. However, these publications do not justify the right amount of plant administered, which can vary greatly. Sideritis hyssopifolia is a little woody plant endemic to western and southwestern Europe. We have quantified its antioxidant activity, which can be used as an indicator of its cardioprotective action. This study evaluates the antioxidant capacity of Sideritis hyssopifolia to design a feed whose hypolipidemic effects are proven in cholesterol-fed New Zealand rabbits. Antioxidant action was assessed in infusions, which were prepared with 1 or 3 g of plant in 200 mL of water by using an ABTS assay and expressed as Ascorbic acid Equivalent Antioxidant Capacity (AEAC). Aqueous infusions with infusion times of 10 min and prepared with 3 g plant exhibited the strongest antioxidant activity. Sideritis hyssopifolia showed an intermediate antioxidant capacity for the concentrations and times of the infusion tested. According to our results, we suggest incorporating 2.36 g of S. hyssopifolia every 150 g of rabbit feeding stuff (15.73 g/kg). This chow decreased cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides levels in cholesterol-fed rabbits, as well as the atherogenic index. This reduction was similar to that obtained with simvastatin.
Potent Anti-Ovarian Cancer with Inhibitor Activities on Both Topoisomerase II and V600EBRAF of Synthesized Substituted Estrone Candidates
Authors: El-Naggar Mohamed , E. Amr Abd El-Galil , Fayed Ahmed A. , A. Elsayed Elsayed , A. Al-Omar Mohamed , Abdalla Mohamed M.
Published: May 2019

A series of 16-(α-alkoxyalkane)-17-hydrazino-estra-1(10),2,4-trien[17,16-c]-3-ol (3a–l) and estra-1(10),2,4-trien-[17,16-c]pyrazoline-3-ol derivatives (4a–d) were synthesized from corresponding arylidines 2a,b which was prepared from estrone 1 as starting material. Condensation of 1 with aldehydes gave the corresponding arylidine derivatives 2a,b which were treated with hydrazine derivatives in alcohols to give the corresponding derivatives 3a–l, respectively. Additionally, treatment of 2a,b with methyl- or phenylhydrazine in ethanolic potassium hydroxide afforded the corresponding N-substituted pyrazoline derivatives 4a–d, respectively. All these derivatives showed potent anti-ovarian cancer both in vitro and in vivo. The mechanism of anti-ovarian cancer was suggested to process via topoisomerase II and V600EBRAF inhibition.
Increasing Selenium and Vitamin E in Dairy Cow Milk Improves the Quality of the Milk as Food for Children
Authors: Saran Netto Arlindo , Salles Márcia Saladini Vieira , Roma Júnior Luiz Carlos , Cozzolino Silvia Maria Franciscato , Gonçalves Maria Teresa Moi , Freitas Júnior José Esler de , Zanetti Marcus Antonio
Published: May 2019

In this study, we investigated the beneficial effects of milk biofortified with antioxidants on the health of children. Two experiments were conducted: experiment one evaluated the milk of 24 Jersey dairy cows (450 ± 25 kg of body weight (BW); 60 ± 30 days in milk dry matter intake (DIM)) given different diet treatments (CON = control diet; COANT = diet with vitamin E and selenium as antioxidants; OIL = diet with sunflower oil; and OANT = diet with sunflower oil containing more vitamin E and selenium as antioxidants), and experiment two evaluated the effect of the milk produced in the first experiment on the health of children (CON = control diet; COANT = diet with vitamin E and selenium as antioxidants; OIL = diet with sunflower oil; OANT = diet with sunflower oil containing more vitamin E and selenium as antioxidants; and SM = skim milk). One hundred children (8 to 10 years old) were evaluated in the second experiment. Blood samples were collected at 0 days of milk intake and 28 and 84 days after the start of milk intake. The cows fed the COANT and OANT diets showed greater selenium and vitamin E concentrations in their milk (p = 0.001), and the children who consumed the milk from those cows had higher concentrations of selenium and vitamin E in their blood (p = 0.001). The platelet (p = 0.001) and lymphocyte (p = 0.001) concentrations were increased in the blood of the children that consumed milk from cows fed the OANT diet compared to those in the children that consumed SM (p = 0.001). The children who consumed milk from cows fed the OIL diet treatment had increased concentrations of low density lipoprotein (LDL) and total cholesterol in their blood at the end of the supplementation period compared to children who consumed SM. The results of this study demonstrate that the consumption of biofortified milk increases the blood concentrations of selenium and vitamin E in children, which may be beneficial to their health.
Body Mass Index and Associated Clinical Variables in Patients with Non-Celiac Wheat Sensitivity
Authors: Mansueto Pasquale , Soresi Maurizio , La Blasca Francesco , Fayer Francesca , D’Alcamo Alberto , Carroccio Antonio
Published: May 2019

Background: Non-Celiac Wheat Sensitivity (NCWS) is still a largely undefined condition, due to the lack of a diagnostic marker. Few data are available about the nutritional characteristics of NCWS patients at diagnosis. Aims: To evaluate the proportion of NCWS patients who were underweight, normal weight, overweight, or obese at diagnosis, and to search for possible correlations between their Body Mass Index (BMI) and other NCWS-related disease characteristics. Patients and Methods: The clinical charts of 145 NCWS patients (125 F, 20 M, mean age 37.1 ± 11.4 years), diagnosed between January 2012 and March 2018, were reviewed. As a comparison, 84 celiac disease (CD) patients (73 F, 11 M, mean age 39.8 ± 13.9 years) were evaluated. All NCWS diagnoses were based on a double-blind placebo-controlled wheat challenge (DBPCWC) method. Results: BMI distribution was similar in the NCWS (6.2% underweight and 15.2% obese subjects) and CD patients (6% underweight and 7.1% obese subjects). Underweight NCWS subjects were significantly younger and had a shorter clinical history than the overweight or obese ones. Unlike the other NCWS patients, none of them had a DQ2 and/or DQ8 haplotype. Overweight and obese NCWS patients were more frequently suffering from associated autoimmune diseases than the other BMI categories (P = 0.05). Compared to the CD controls, NCWS patients showed a higher frequency of Irritable Bowel Syndrome (IBS)-like (P = 0.01) and extraintestinal symptoms (P = 0.03) and a longer clinical history (P = 0.04), whereas weight loss was more frequent in CD (P = 0.02). Conclusions: NCWS patients showed a BMI distribution similar to CD patients. However, NCWS was found to be a heterogenous condition that regards BMI, and clinical characteristics differed between the underweight and overweight/obese patients.
Habitual Breakfast Patterns Do Not Influence Appetite and Satiety Responses in Normal vs. High-Protein Breakfasts in Overweight Adolescent Girls
Authors: Douglas Steve M. , Byers Adam W. , Leidy Heather J.
Published: May 2019

Differences in postprandial insulin, glucose, and/or free fatty acid concentrations, following the consumption of breakfast, have been demonstrated to be dependent on habitual breakfast patterns. This study examined the effects of habitual breakfast patterns on postprandial appetite, satiety, and hormonal responses along with daily food intake following the consumption of normal-protein (NP) vs. higher-protein (HP) breakfasts in overweight adolescents. Thirty-seven girls (age: 19 ± 1 year; BMI: 29.0 ± 3.4 kg/m2) participated in the semi-randomized crossover design study. Participants were grouped according to whether they habitually skipped (SKIP, n = 18) or consumed breakfast (CONSUME, n = 19), and consumed a NP (350 kcal; 13 g protein) or HP (350 kcal; 35 g protein) breakfast for 3 days/pattern. On day 4, breakfast was provided, and appetite questionnaires and blood samples were collected throughout an 8 h testing day. Daily food intake was also assessed. Regardless of habitual breakfast patterns, the consumption of HP breakfast led to greater daily fullness (29,030 ± 6,010 min × mm) vs. NP breakfast (26,910 ± 5580 min × mm; p = 0.03). Daily protein consumption was greater (98 ± 15 g vs. 78 ± 15 g), and carbohydrate consumption was lower (331 ± 98 g vs. 367 ± 94 g) with HP vs. NP (both, p < 0.001). No other differences were observed. These data suggest that the recommendation to consume a HP breakfast for improved satiety and ingestive behavior is appropriate for overweight adolescent girls, regardless of habitual breakfast patterns.
Preventive Effects and Mechanisms of Garlic on Dyslipidemia and Gut Microbiome Dysbiosis
Authors: Chen Keyu , Xie Kun , Liu Zhuying , Nakasone Yasushi , Sakao Kozue , Hossain Md. Amzad , Hou De-Xing
Published: May 2019

Garlic (Allium sativum L.) contains prebiotic components, fructans, antibacterial compounds, and organosulfur compounds. The complex ingredients of garlic seem to impart a paradoxical result on the gut microbiome. In this study, we used a mouse model to clarify the effects of whole garlic on the gut microbiome. C57BL/6N male mice were fed with or without whole garlic in normal diet (ND) or in high-fat diet (HFD) for 12 weeks. Supplementation with whole garlic attenuated HFD-enhanced ratio of serum GPT/GOT (glutamic-pyruvic transaminase/glutamic-oxaloacetic transaminase), levels of T-Cho (total cholesterol) and LDLs (low-density lipoproteins), and index of homeostatic model assessment for insulin resistance (HOMA-IR), but had no significant effect in the levels of serum HDL-c (high density lipoprotein cholesterol), TG (total triacylglycerol), and glucose. Moreover, garlic supplementation meliorated the HFD-reduced ratio of villus height/crypt depth, cecum weight, and the concentration of cecal organic acids. Finally, gut microbiota characterization by high throughput 16S rRNA gene sequencing revealed that whole garlic supplementation increased the α-diversity of the gut microbiome, especially increasing the relative abundance of f_Lachnospiraceae and reducing the relative abundance of g_Prevotella. Taken together, our data demonstrated that whole garlic supplementation could meliorate the HFD-induced dyslipidemia and disturbance of gut microbiome.
Glycemic Index of Slowly Digestible Carbohydrate Alone and in Powdered Drink-Mix
Authors: Gourineni Vishnupriya , Stewart Maria L. , Skorge Rob , Wolever Thomas
Published: May 2019

Consumer interest in food and beverages with carbohydrates offering steady glucose release and lower glycemic index (GI) continues to rise. Glycemic index is one of the metrics for carbohydrate quality. Slowly digestible carbohydrates (SDC) offer an ingredient solution to improve carbohydrate quality and meet consumer needs. SUSTRATM 2434 slowly digestible carbohydrate is a blend of tapioca flour and corn starch. The study objective was to determine the glycemic index of the SDC ingredient alone and in a powdered drink-mix. In a randomized, single-blind study, heathy adults (n = 14) consumed four test drinks, delivering 50 g available carbohydrates on separate days to measure GI. Participants either consumed dextrose in water (placebo), SDC ingredient in water, SDC drink-mix powder reconstituted in skim milk, or control drink-mix reconstituted in skim milk (without SDC). Post-prandial glucose response was measured over 4 h. SDC exhibited lower GI (0–2 h) and higher steady glucose release (beyond 2 h). SDC alone (GI = 27) and SDC in drink-mix (GI = 30.3) showed significantly lower GI (−27%) compared to dextrose (100) and the control drink-mix (41.5). SUSTRATM 2434 SDC is a low glycemic ingredient, suitable for product innovations with potential for low glycemic and steady glucose release claims.
Tree Height Estimation of Forest Plantation in Mountainous Terrain from Bare-Earth Points Using a DoG-Coupled Radial Basis Function Neural Network
Authors: He Haiqing , Yan Yeli , Chen Ting , Cheng Penggen
Published: May 2019

Tree heights are the principal variables for forest plantation inventory. The increasing availability of high-resolution three-dimensional (3D) point clouds derived from low-cost Unmanned Aerial Vehicle (UAV) and modern photogrammetry offers an opportunity to generate a Canopy Height Model (CHM) in the mountainous areas. In this paper, we assessed the capabilities of tree height estimation using UAV-based Structure-from-Motion (SfM) photogrammetry and Semi-Global Matching (SGM). The former is utilized to generate 3D geometry, while the latter is used to generate dense point clouds from UAV imagery. The two algorithms were coupled with a Radial Basis Function (RBF) neural network to acquire CHMs in mountainous areas. This study focused on the performance of Digital Terrain Model (DTM) interpolation over complex terrains. With the UAV-based image acquisition and image-derived point clouds, we constructed a 5 cm-resolution Digital Surface Model (DSM), which was assessed against 14 independent checkpoints measured by a Real-Time Kinematic Global Positioning System RTK GPS. Results showed that the Root Mean Square Errors (RMSEs) of horizontal and vertical accuracies are approximately 5 cm and 10 cm, respectively. Bare-earth Index (BEI) and Shadow Index (SI) were used to separate ground points from the image-derived point clouds. The RBF neural network coupled with the Difference of Gaussian (DoG) was exploited to provide a favorable generalization for the DTM from 3D ground points with noisy data. CHMs were generated using the height value in each pixel of the DSM and by subtracting the corresponding DTM value. Individual tree heights were estimated using local maxima algorithm under a contour-surround constraint. Two forest plantations in mountainous areas were selected to evaluate the accuracy of estimating tree heights, rather than field measurements. Results indicated that the proposed method can construct a highly accurate DTM and effectively remove nontreetop maxima. Furthermore, the proposed method has been confirmed to be acceptable for tree height estimation in mountainous areas given the strong linear correlation of the measured and estimated tree heights and the acceptable t-test values. Overall, the low-cost UAV-based photogrammetry and RBF neural network can yield a highly accurate DTM over mountainous terrain, thereby making them particularly suitable for rapid and cost-effective estimation of tree heights of forest plantation in mountainous areas.
Coastal Waveform Retracking in the Slick-Rich Sulawesi Sea of Indonesia, Based on Variable Footprint Size with Homogeneous Sea Surface Roughness
Authors: Wang Xifeng , Ichikawa Kaoru , Wei Dongni
Published: May 2019

Waveforms of radar altimeters are often corrupted due to heterogeneous sea surface roughness within footprints, such as slicks. In past studies, subwaveform retrackers such as the adaptive leading edge subwaveform retracker (ALES) which use only a section of the waveform have been proposed. However, it is difficult to choose a reasonable estimation window from an individual waveform. In the present study, a post-processed subwaveform retracker is proposed which identifies the waveforms of surrounding along-track points. The size of the estimation window is variable and is determined to keep the sea surface roughness within the corresponding footprint homogeneous. The method was applied to seven years of 20 Hz Jason-2 altimeter data over the slick-rich Sulawesi Sea of Indonesia and compared with ALES and sensor geophysical data record (SGDR) products. The standard deviation of the sea surface dynamic heights was around 0.13 m, even without spatial smoothing or some geophysical corrections. This is only 75% and 25% of the ALES and SGDR results, respectively. Moreover, all retrievals of the range, SWH, and sigma0 include less outliers than the other products. These results indicate that the variable estimation windows determined in the present study can adapt well to the variation of sea surface roughness.
Evaluation of Soil Moisture Variability in Poland from SMOS Satellite Observations
Authors: Usowicz Bogusław , Lipiec Jerzy , Lukowski Mateusz
Published: May 2019

Soil moisture (SM) data play an important role in agriculture, hydrology, and climate sciences. In this study, we examined the spatial-temporal variability of soil moisture using Soil Moisture Ocean Salinity (SMOS) satellite measurements for Poland from a five-year period (2010–2014). SMOS L2 v. 551 datasets (latitudinal rectangle 1600 × 840 km, centered in Poland) averaged for quarterly (three months corresponding to winter, spring, summer, and autumn) and yearly values were used. The results were analysed with the use of classical statistics and geostatistics (using semivariograms) to acquire information about the nature of anisotropy and the lengths and directions of spatial dependences. The minimum (close to zero) and maximum soil moisture values covered the 0.5 m3 m−3 range. In particular quarters, average soil moisture did not exceed 0.2 m3 m−3 and did not drop below 0.12 m3 m−3; the corresponding values in the study years were 0.171 m3 m−3 and 0.128 m3 m−3. The highest variability of SM occurred generally in winter (coefficient of variation, CV, up to 40%) and the lowest value was recorded in spring (around 23%). The average CV for all years was 32%. The quarterly maximum (max) soil moisture contents were well positively correlated with the average soil moisture contents (R2 = 0.63). Most of the soil moisture distributions (histograms) were close to normal distribution and asymmetric data were transformed with the square root to facilitate geostatistical analysis. Isotropic and anisotropic empirical semivariograms were constructed and the theoretical exponential models were well fitted (R2 > 0.9). In general, the structural dependence of the semivariance was strong and moderate. The nugget (C0) values slightly deceased with increasing soil moisture while the sills (C0 + C) increased. The effective ranges of spatial dependence (A) were between 1° and 4° (110–440 km of linear distance). Generally, the ranges were greater for drier than moist soils. Anisotropy of the SM distribution exhibited different orientation with predominance from north-west to south-east in winter and spring and changed for from north-east to south-west or from north to south in the other seasons. The fractal dimension values showed that the distribution of the soil moisture pattern was less diverse (smoother) in the winter and spring, compared to that in the summer and autumn. The soil moisture maps showed occurrence of wet areas (soil moisture > 0.25 m3 m−3) in the north-eastern, south-eastern and western parts and dry areas (soil moisture < 0.05 m3 m−3) mainly in the central part (oriented towards the south) of Poland. The spatial distribution of SM was attributed to soil texture patterns and associated with water holding capacity and permeability. The results will help undertake appropriate steps to minimize susceptibility to drought and flooding in different regions of Poland.
Spatio-Temporal Pattern Change of Winter Wheat Production and Its Implications in the North China Plain
Authors: Zhang Zemin , Lu Changhe
Published: May 2019

The North China Plain (NCP) is the most important winter wheat production region and an area of water shortage in China. The stability of winter wheat (T. aestivum L.) production in spatial pattern and the sustainability of water resources have been a major policy concern in China. This study explored the barycenter shift and change trends of wheat total production during 1998–2015, using methods of barycenter model, Sen’s slope, and Mann Kendall test, and analyzed the influence of external factors and the response of water resources. Results indicated that the barycenter of wheat production moved southwards by 115.16 km during 1998–2015, with an average speed of 6.77 km/year. For the entire NCP, the total production showed phased changes during the study period: It decreased during 1998–2003, and then continuously increased during 2004–2015. Of the wheat production increase in the NCP, yield increase and sown area expansion averagely contributed 64.5% and 35.5%, respectively, and the contribution proportion of yield increase continuously increased since 2003. At county level, total wheat production showed a significant increase and decrease trend in 87 and 29 counties, mainly distributed in the southern and northern NCP, respectively. The increase of total production at county level was mainly contributed by yield growth in the southern NCP, while the decrease in the north was due to the reduction of sown area to great extent. The southward shift was jointly resulted by the spatial variation of input factors, benefit, and water prices. These spatial pattern changes alleviated the water pressure in the north region to some extent, in the case of ensuring the production increase of winter wheat. Therefore, the current spatial shift should be continuously promoted in the future.
Perception of Embodied Carbon Mitigation Strategies: The Case of Sri Lankan Construction Industry
Authors: Willhelm Abeydeera Lebunu Hewage Udara , Wadu Mesthrige Jayantha , Samarasinghalage Tharushi Imalka
Published: May 2019

Whilst operational carbon (OC) emission reduction has received greater attention in the literature, embodied carbon (EC) emission reduction aspect has been largely neglected. This is particularly seen in developing countries. This study assessed the awareness and perception of carbon emission in general and EC emission reduction strategies in particular in the Sri Lankan construction industry. A detailed questionnaire, based on a comprehensive literature review, was developed to assess the awareness and perception of the Sri Lankan construction professionals about global carbon emissions, OC emissions, and EC emissions and carbon mitigation strategies. Based on a sample of 111 professionals in the construction sector, results revealed that the Sri Lankan construction professionals have poor awareness about carbon emission, especially about EC emission and EC mitigation strategies. The results further revealed that they are more concerned about the OC emission reduction than the EC emission reduction. The results suggest that they are basically aware of some basic/conventional mitigation strategies such as better design (low-carbon), an extension of building life and refurbishment of existing buildings and carbon tax, but their awareness of recently introduced micro-level technologies/strategies is significantly poor. Findings are a clear reflection of the current situation in many developing countries with regard to carbon emission and mitigation strategies. It was found that a major reason for low awareness was related to the culture: The majority of the respondents believed that actions to reduce carbon footprint should be initiated and handled by the government and other authorities, but not by construction professionals.
Sustainable Production of Sweet Sorghum as a Bioenergy Crop Using Biosolids Taking into Account Greenhouse Gas Emissions
Authors: Głąb Lilianna , Sowiński Józef
Published: May 2019

Currently, little data are available on greenhouse gas (GHG) emissions from sweet sorghum production under temperate climate. Similarly, information on the effect of bio-based waste products use on the carbon (C) footprint of sorghum cultivation is rare in the literature. The aim of this study was to evaluate the agronomical and environmental effects of the application of biosolids as a nitrogen source in the production of sweet sorghum as a bioenergy crop. The yield of sorghum biomass was assessed and the GHG emissions arising from crop production were quantified. The present study focused on whether agricultural use of sewage sludge and digestate could be considered an option to improve the C footprint of sorghum production. Biosolids—sewage sludge and digestate—could be recognized as a nutrient substitute without crop yield losses. Nitrogen application had the greatest impact on the external GHG emissions and it was responsible for 54% of these emissions. CO2eq emissions decreased by 14 and 11%, respectively, when sewage sludge and digestate were applied. This fertilization practice represents a promising strategy for low C agriculture and could be recommended to provide sustainable sorghum production as a bioenergy crop to mitigate GHG emissions.
Sustainable Developmental Evaluation of Foreign Trade Based on Emergy Analysis Method in Shenzhen City, China
Authors: Tian Yutong , Xue Shulian , Li Chunhui , Liang Hongli , Mao Jiansu
Published: May 2019

The foreign trade sustainable development index system of Shenzhen City, including the three subsystems of environment, economy, and society, was constructed based on the theory of emergy analysis. The sustainable development of foreign trade in Shenzhen City from 2009 to 2016 was evaluated, and a detailed analysis of changes in the emergy of light and heavy industries was performed. The results showed that the scale of economy has been expanding, and the total volume of imports and exports has turned from a rise to a decline in 2013. The status of sustainable development is not optimistic. The transaction volume of energy is reduced, and the quality of people’s living environment is declining. The sustainable development of Shenzhen City is not perfect, but it is in a phase of gradual optimization. Moreover, the proportion of heavy industry in import and export trade is significantly higher than that of light industry, which has caused the outflow of energy to a certain extent. Therefore, to improve the level of foreign trade sustainable development, the efficiency of resource utilization must be improved. The import of energy products must be increased, the ability to cope with external interference must be strengthened, and the foreign trade industrial structure must be adjusted.
The Sustainability of Post-Conflict Development: The Case of Algeria
Authors: Mourad Khaldoon A. , Avery Helen
Published: May 2019

The Algerian civil war, 1992–2002, affected all aspects of life in the country. Major development efforts were therefore initiated in the post-conflict era. Almost 20 years later, the economy remains fragile, and the country’s large hydrocarbon revenues have not been used to develop the infrastructure for sustainability, support energy transition or reduce structural vulnerabilities. This paper provides an overview of Algerian development strategies before and after the conflict, examining in particular the orientation of major development projects involving foreign financing. Two rural development programmes are described to illustrate the outcomes of such projects. The results show that the conflict stopped or hindered many ongoing and planned development projects in the country, especially in the agriculture sector, while new investments in industry started after the conflict. The review of individual development projects further revealed that many projects between 1980–2017 had doubtful benefits with respect to long-term development goals. Initiatives tended to be discontinued once the funding period closed, and the involvement of the private sector was low. It is therefore concluded that additional attention needs to be devoted to long-term and structural impacts of development projects, including considerations regarding sustainability, demographics, and climate-related future changes.