bluuebunny/crossref_metadata_2025 huggingface dataset:
A Model-Based Sensor Fault Diagnosis Scheme for Batteries in Electric Vehicles
Authors: Yu Quanqing , Wan Changjiang , Li Junfu , Xiong Rui , Chen Zeyu
Published: February 2021

The implementation of each function of a battery management system (BMS) depends on sensor data. Efficient sensor fault diagnosis is essential to the durability and safety of battery systems. In this paper, a model-based sensor fault diagnosis scheme and fault-tolerant control strategy for a voltage sensor and a current sensor are proposed with recursive least-square (RLS) and unscented Kalman filter (UKF) algorithms. The fault diagnosis scheme uses an open-circuit voltage residual generator and a capacity residual generator to generate multiple residuals. In view of the different applicable state of charge (SOC) intervals of each residual, different residuals need to be selected according to the different SOC intervals to evaluate whether a sensor fault occurs during residual evaluation. The fault values of the voltage and current sensors are derived in detail based on the open-circuit voltage residual and the capacity residual, respectively, and applied to the fault-tolerant control of battery parameters and state estimations. The performance of the proposed approaches is demonstrated and evaluated by simulations with MATLAB and experimental studies with a commercial lithium-ion battery cell.
The Combustion Characteristics of Double Ramps in a Strut-Based Scramjet Combustor
Authors: Athithan A. Antony , Jeyakumar S. , Sczygiol Norbert , Urbanski Mariusz , Hariharasudan A.
Published: February 2021

This paper focuses on the influence of ramp locations upstream of a strut-based scramjet combustor under reacting flow conditions that are numerically investigated. In contrast, a computational study is adopted using Reynolds Averaged Navier Stokes (RANS) equations with the Shear Stress Transport (SST) k-ω turbulence model. The numerical results of the Deutsches Zentrum für Luft- und Raumfahrt or German Aerospace Centre (DLR) scramjet model are validated with the reported experimental values that show compliance within the range, indicating that the adopted simulation method can be extended for other investigations as well. The performance of the ramps in the strut-based scramjet combustor is analyzed based on parameters such as wall pressures, combustion efficiency and total pressure loss at various axial locations of the combustor. From the numerical shadowgraph, more shock interactions are observed upstream of the strut injection region for the ramp cases, which decelerates the flow downstream, and additional shock reflections with less intensity are also noticed when compared with the DLR scramjet model. The shock reflection due to the ramps enhances the hydrogen distribution in the spatial direction. The ignition delay is noticed for ramp combustors due to the deceleration of flow compared to the baseline strut only scramjet combustor. However, a higher flame temperature is observed with the ramp combustor. Because more shock interactions arise from the ramps, a marginal increase in the total pressure loss is observed for ramp combustors when compared to the baseline model.
Drivers and Barriers to the Adoption of Fuel Cell Passenger Vehicles and Buses in Germany
Authors: Trencher Gregory , Edianto Achmed
Published: February 2021

As policymakers and automotive stakeholders around the world seek to accelerate the electrification of road transport with hydrogen, this study focuses on the experiences of Germany, a world leader in fuel cell technology. Specifically, it identifies and compares the drivers and barriers influencing the production and market penetration of privately-owned fuel cell electric passenger vehicles (FCEVs) and fuel cell electric buses (FCEBs) in public transit fleets. Using original data collected via a survey and 17 interviews, we elicited the opinions of experts to examine opportunities and obstacles in Germany from four perspectives: (i) the supply of vehicles (ii) refuelling infrastructure, (iii) demand for vehicles, and (iv) cross-cutting institutional issues. Findings indicate that despite multiple drivers, there are significant challenges hampering the growth of the hydrogen mobility market. Several are more pronounced in the passenger FCEV market. These include the supply and cost of production, the lack of German automakers producing FCEVs, the profitability and availability of refuelling stations, and low demand for vehicles. In light of these findings, we extract implications for international policymakers and future studies. This study provides a timely update on efforts to spur the deployment of hydrogen mobility in Germany and addresses the underrepresentation of studies examining both buses and passenger vehicles in tandem.
A Matching Problem between the Front Fan and Aft Fan Stages in Adaptive Cycle Engines with Convertible Fan Systems
Authors: Meng Xin , Zhu Zhili , Chen Min , Xu Yihao
Published: February 2021

In the process of studying the steady-state performance and component matching of adaptive cycle engines with convertible fan system, it was found that the front fan and aft fan stage have a unique matching problem when the mode select valve is closed and engine is operating at higher Mach number conditions. The cause of this matching problem was studied with numeric simulation in this paper. Based on the features of adaptive cycle engines with convertible fan system, the possible methods and their feasibilities of solving this matching problem were also discussed. According to the results, the flow rate adjustment capacity of the aft fan stage directly determines the occurrence and severity of this matching problem. The matching problem can be ameliorated in some extent by either reducing the design second bypass ratio or adjusting the variable geometry mechanisms, but it cannot be completely solved at the aspect of component matching mechanism.
PV Systems Control Using Fuzzy Logic Controller Employing Dynamic Safety Margin under Normal and Partial Shading Conditions
Authors: Bakkar Mostafa , Aboelhassan Ahmed , Abdelgeliel Mostafa , Galea Michael
Published: February 2021

Because of the unpredictable activity of solar energy sources, photovoltaic (PV) maximum power point tracking (MPPT) is essential to guarantee the continuous operation of electrical energy generation at optimal power levels. Several works have extensively examined the generation of the maximum power from the PV systems under normal and shading conditions. The fuzzy logic control (FLC) method is one of the effective MPPT techniques, but it needs to be adapted to work in partial shading conditions. The current paper presents the FLC-based on dynamic safety margin (DSM) as an MPPT technique for a PV system to overcome the limitations of FLC in shading conditions. The DSM is a performance index that measures the system state deviation from the normal situation. As a performance index, DSM is used to adapt the FLC controller output to rapidly reach the global maxima of the PV system. The ability of the proposed algorithm and its performance are evaluated using simulation and practical implementation results for single phase grid-connected PV system under normal and partial shading operating conditions.
Optimally Clocking the Low Carbon Energy Mile to Achieve the Sustainable Development Goals: Evidence from Dundee’s Electric Vehicle Strategy
Authors: Asekomeh Ayodele , Gershon Obindah , Azubuike Smith I.
Published: February 2021

Dundee City has been successful in installing green infrastructure for charging electric vehicles (EVs). This intervention matches the Sustainable Development Goals (SDGs) of affordable clean energy (SDG 7), sustainable cities and communities (SDG 11) and climate action (SDG 13) of the United Nations General Assembly Agenda 2030 (Transforming our World: the 2030 Agenda for Sustainable Development). Local authorities can align interventions with SDGs according to needs. The purpose of this paper is to consider whether Dundee’s EV strategy represents the most viable and equitable intervention that could be adopted given the city’s context. We adopt a positive review and value argumentation approach to determine the extent to which the strategy satisfies the criteria of “level of urgency”, “systemic impact” and “policy gaps”, which have been employed in the extant literature as the basis for a multi-criteria analysis (MCA). We eclectically review elements of the strategy against the city’s peculiar physical and socio-economic environment, as well as argue their fit against these criteria. We interpret these criteria based on the complementarity and benefits of the strategy from the lenses of SDG 7, SDG 11 and SDG 13. Additionally, we consider the alignment of the EV strategy with the other SDGs. The criteria also allow us to evaluate the strategy based on the localisation principles of equity, acceptability and affordability of the intervention. Our review shows that the EV strategy represents a sustainable and community life-enhancing intervention that aligns with some key SDGs. However, the outcome raises concerns about the equitability of the strategy. Smaller, similar or bigger cities could utilise this approach. However, we recommend the evaluation of local priorities to improve alignment with the SDGs and the provision of clear justifications for selecting an intervention from a range of responses.
Numerical Study of Bloodstream Diffusion of the New Generation of Drug-Eluting Stents in Coronary Arteries
Authors: Marques Leandro , R. Anjos Gustavo
Published: February 2021

The present work aims at developing a numerical study on the drug diffusion in the bloodstream in a coronary artery with drug-eluting stent implanted. The blood was modeled as a single-phase, incompressible and Newtonian fluid and the Navier–Stokes equation was approximated according to the Finite Element Method (FEM). The dynamics of drug-eluting concentration in bloodstream was investigated using four drug-eluting stents with different mass diffusivities in microchannels with variable cross sections, including a real coronary artery geometry with atherosclerosis. The results reveal complex drug concentration patterns and accumulation in the vicinity of the fat buildup.
In Vitro Evaluation of the Effect of a Not Cross-Linked Hyaluronic Acid Hydrogel on Human Keratinocytes for Mesotherapy
Authors: Zerbinati Nicola , Sommatis Sabrina , Maccario Cristina , Capillo Maria Chiara , Di Francesco Serena , Rauso Raffaele , Protasoni Marina , D’Este Edoardo , Dalla Gasperina Daniela , Mocchi Roberto
Published: February 2021

The multicomponent preparations for mesotherapy are based on the principle that skin and hair aging can be prevented by supplying the fundamental substrates for correct cellular functioning, such as nucleotides, vitamins, amino acids, and biomolecules including hyaluronic acid (HA) that promote skin hydration and several biological activities. The study provides evidence for the application of HYDRO DELUXE BIO (Matex Lab S.p.A), a biocompatible hydrogel containing not cross-linked HA, for the treatment of the scalp’s skin by mesotherapy. Using an in vitro model of immortalized human keratinocytes, we studied markers involved in hair aging prevention and growth, such as inflammatory markers, angiogenesis, and oxidative damage. HYDRO DELUXE BIO showed high biocompatibility and the ability to significantly reduce the expression of the inflammation marker interleukin (IL)-8 in Tumor Necrosis Factor (TNF)-stimulated cells. Then, we evaluated angiogenesis, a pivotal event during hair growth, measuring the Vascular Endothelial Growth Factor (VEGF) expression that resulted to be significantly increased in treated cells, suggesting a pro-angiogenetic capability. A protective activity against the oxidative stress agent was showed, increasing the survival rate in treated cells. Concluding, HYDRO DELUXE BIO is suitable for treatment by mesotherapy of the scalp’s skin as it modulates the expression levels of markers involved in the biorevitalization of the hair follicle.
Whole Exome Sequencing Reveals a Novel AUTS2 In-Frame Deletion in a Boy with Global Developmental Delay, Absent Speech, Dysmorphic Features, and Cerebral Anomalies
Authors: Palumbo Pietro , Di Muro Ester , Accadia Maria , Benvenuto Mario , Di Giacomo Marilena Carmela , Castellana Stefano , Mazza Tommaso , Castori Marco , Palumbo Orazio , Carella Massimo
Published: February 2021

Neurodevelopmental disorders (NDDs) are a group of highly prevalent, clinically and genetically heterogeneous pediatric disorders comprising, according to the Diagnostic and Statistical Manual of Mental Disorders 5th edition (DSM-V), intellectual disability, developmental delay, autism spectrum disorders, and other neurological and cognitive disorders manifesting in the developmental age. To date, more than 1000 genes have been implicated in the etiopathogenesis of NNDs. Among them, AUTS2 (OMIM # 607270) encodes a protein involved in neural migration and neuritogenesis, and causes NNDs with different molecular mechanisms including copy number variations, single or multiple exonic deletion and single nucleotide variants. We describes a 9-year-old boy with global developmental delay, absent speech, minor craniofacial anomalies, hypoplasia of the cerebellar vermis and thinning of the corpus callosum, resulted carrier of the de novo AUTS2 c.1603_1626del deletion at whole exome sequencing (WES) predicted to cause the loss of eight amino acids [p.(His535_Thr542del)]. Notably, our patient is the first reported so far in medical literature carrying an in-frame deletion and the first in which absent language, hypoplasia of the cerebellar vermis and thinning of the corpus callosum has been observed thus useful to expand the molecular spectrum of AUTS2 pathogenic variants and to broaden our knowledge on the clinical phenotype associated.
TERT Promoter Mutation Analysis of Whole-Organ Mapping Bladder Cancers
Authors: Weyerer Veronika , Eckstein Markus , Strissel Pamela L. , Wullweber Adrian , Lange Fabienne , Tögel Lars , Geppert Carol I. , Sikic Danijel , Taubert Helge , Wach Sven , Wullich Bernd , Hartmann Arndt , Stoehr Robert , Giedl Johannes
Published: February 2021

Background: Multifocal occurrence is a main characteristic of urothelial bladder cancer (UBC). Whether urothelial transformation is caused by monoclonal events within the urothelium, or by polyclonal unrelated events resulting in several tumor clones is still under debate. TERT promoter mutations are the most common somatic alteration identified in UBC. In this study, we analyzed different histological tissues from whole-organ mapping bladder cancer specimens to reveal TERT mutational status, as well as to discern how tumors develop. Methods: Up to 23 tissues from nine whole-organ mapping bladder tumor specimens, were tested for TERT promoter mutations including tumor associated normal urothelium, non-invasive urothelial lesions (hyperplasia, dysplasia, metaplasia), carcinoma in situ (CIS) and different areas of muscle invasive bladder cancers (MIBC). The mutational DNA hotspot region within the TERT promoter was analyzed by SNaPshot analysis including three hot spot regions (−57, −124 or −146). Telomere length was measured by the Relative Human Telomere Length Quantification qPCR Assay Kit. Results: TERT promoter mutations were identified in tumor associated normal urothelium as well as non-invasive urothelial lesions, CIS and MIBC. Analysis of separate regions of the MIBC showed 100% concordance of TERT promoter mutations within a respective whole-organ bladder specimen. Polyclonal events were observed in five out of nine whole-organ mapping bladder cancers housing tumor associated normal urothelium, non-invasive urothelial lesions and CIS where different TERT promoter mutations were found compared to MIBC. The remaining four whole-organ mapping bladders were monoclonal for TERT mutations. No significant differences of telomere length were observed. Conclusions: Examining multiple whole-organ mapping bladders we conclude that TERT promoter mutations may be an early step in bladder cancer carcinogenesis as supported by TERT mutations detected in tumor associated normal urothelium as well as non-invasive urothelial lesions. Since mutated TERT promoter regions within non-invasive urothelial lesions are not sufficient alone for the establishment of cancerous growth, this points to the contribution of other gene mutations as a requirement for tumor development.
Presence of SARS-CoV-2 RNA in Semen—Cohort Study in the United States COVID-19 Positive Patients
Authors: Machado Bruno , Barcelos Barra Gustavo , Scherzer Nickolas , Massey Jack , dos Santos Luz Hemerson , Henrique Jacomo Rafael , Herinques Santa Rita Ticiane , Davis Rodney
Published: February 2021

On 31 December 2019, China informed the World Health Organization they were facing a viral pneumonia epidemic of a new type of Coronavirus. Currently, 10 months later, more than 43,000,000 people have been infected, and about 1,150,000 deceased worldwide from the disease. Knowledge about the virus is updated daily, and its RNA was isolated from several human secretions, e.g., throat, saliva, pulmonary alveolar washing, and feces. So far, only one publication found the presence of SARS-CoV-2 in semen. In this 5-month cross-sectional study, we recruited 15 patients diagnosed with a positive nasal swab for SARS-CoV-2 with no or mild symptoms in our institution. A semen sample after a shower was retrieved and tested for viral RNA in the semen. The samples were tested for the viral RNA with RT-PCR with two different genetic probes. The samples were re-tested 24 h after the first test to confirm the results. The SARS-Cov-2 viral RNA was present in 1/15 patients [6.66%] in our sample. Even in a small sample, the RNA from SARS-CoV-2 can be isolated from human semen. This information should alert the scientific community and public health officials about a possible new form of transmission of the disease and long-term clinical effects on the population.
Prevalence and Determinants of Medication Adherence among Patients with HIV/AIDS in Southern Vietnam
Authors: Nguyen Phuong M. , Thach Anh N. , Pham Xuan D. , Lam Anh N. , Nguyen Thao N. P. , Duong Chu X. , Nguyen Lam V. , Nguyen Thao H. , Pham Suol T. , Taxis Katja , Nguyen Thang
Published: February 2021

This study was conducted to determine the prevalence and determinants of medication adherence among patients with HIV/AIDS in southern Vietnam. Methods: A cross-sectional study was conducted in a hospital in southern Vietnam from June to December 2019 on patients who began antiretroviral therapy (ART) for at least 6 months. Using a designed questionnaire, patients were considered adherent if they took correct medicines with right doses, on time and properly with food and beverage and had follow-up visits as scheduled. Multivariable logistic regression was used to identify determinants of adherence. Key findings: A total of 350 patients (from 861 medical records) were eligible for the study. The majority of patients were male (62.9%), and the dominant age group (≥35 years old) accounted for 53.7% of patients. Sexual intercourse was the primary route of transmission of HIV (95.1%). The proportions of participants who took the correct medicine and at a proper dose were 98.3% and 86.3%, respectively. In total, 94.9% of participants took medicine appropriately in combination with food and beverage, and 75.7% of participants were strictly adherent to ART. The factors marital status (odds ratio (OR) = 2.54; 95%CI = 1.51–4.28), being away from home (OR = 1.7; 95%CI = 1.03–2.78), substance abuse (OR = 2.7; 95%CI = 1.44–5.05), general knowledge about ART (OR = 2.75; 95%CI = 1.67–4.53), stopping medication after improvement (OR = 4.16; 95%CI = 2.29–7.56) and self-assessment of therapy adherence (OR = 9.83; 95%CI = 5.44–17.77) were significantly associated with patients’ adherence. Conclusions: Three-quarters of patients were adherent to ART. Researchers should consider these determinants of adherence in developing interventions in further studies.
Self-Medication of Drugs in Nursing Students from Castile and Leon (Spain)
Authors: Galán Andrés Maria Isabel , Guijo Blanco Valeriana , Casado Verdejo Inés , Iglesias Guerra José Antonio , Fernández García Daniel
Published: February 2021

To determine the prevalence of self-medication in nursing students and their related factors, a transversal, descriptive study was performed on a sample of 378 nursing students. A total of 73.8% of the sample declared having used off-prescription drugs during the last month (2.84;2.26–3.58). A total of 28.9% said they did this because they are familiar with the health problem and its pharmacological solution and 25% deemed that it was a mild health problem. Drugs most commonly used off-prescription were analgesics in 88.91% (3.63;2.74–4.80) of occasions. They were mainly recommended by the students’ family (1.31;1.03–1.65) on 58.12% of the cases. Students keep analgesics they take off-prescription in their home first aid kit (4.47;3.28–6.08; p < 0.001). Unlike other studies, 53.2% obtained off-prescription drugs from the home first aid kit (1.13;0.89–1.43; p < 0.001). In addition, they gave advice and recommend drugs they have taken to other people with similar symptoms (1.97;1.59–2.44). A total of 85.72% kept excess drugs after a treatment (6.00;4.50–7.99). Self-medication is related to the storage of unused medicines and giving advice on the use of drugs to other people, among other things. Self-medication of drugs among nursing students is high. Thus, it appears necessary to review the training on rational the use of drugs and responsible self-medication in the discipline’s curriculum.
The Role of Societal Aspects in the Formation of Official COVID-19 Reports: A Data-Driven Analysis
Authors: Kurbucz Marcell Tamás , Katona Attila Imre , Lantos Zoltán , Kosztyán Zsolt Tibor
Published: February 2021

This paper investigates the role of socioeconomic considerations in the formation of official COVID-19 reports. To this end, we employ a dataset that contains 1159 pre-processed indicators from the World Bank Group GovData360 and TCdata360 platforms and an additional 8 COVID-19 variables generated based on reports from 138 countries. During the analysis, a rank-correlation-based complex method is used to identify the time- and space-varying relations between pandemic variables and the main topics of World Bank Group platforms. The results not only draw attention to the importance of factors such as air traffic, tourism, and corruption in report formation but also support further discipline-specific research by mapping and monitoring a wide range of such relationships. To this end, a source code written in R language is attached that allows for the customization of the analysis and provides up-to-date results.
Social Prestige of the Paramedic Profession
Authors: Majchrowska Anita , Pawlikowski Jakub , Jojczuk Mariusz , Nogalski Adam , Bogusz Renata , Nowakowska Luiza , Wiechetek Michał
Published: February 2021

Background: There is a lack of research on social image, prestige, and the position of the paramedic profession in the social structure. The main objective of the study was to determine the place of the paramedic profession in the hierarchy of prestige of professions as viewed by the public. In operationalizing the term ‘prestige’, we deemed the word ‘respect’ to best fit the sense of the subjective evaluation of prestige with regard to a profession. Material and methods: The data comes from cross-sectional survey-based research. The research was carried out on a group of 600 people over 18 years of age. The sample was of a random nature, and the selection of respondents was calculated on the basis of them being representative of the Polish population. Results: The median of respect declared for the paramedic profession, on a scale of 1 to 5, was 4.49, which placed the profession in fourth place in the ranking. The assessment of respect for paramedics among other medical professions placed them in third place, directly after doctors and midwifes. Conclusions: The profession of paramedic is characterized by high social prestige, locating it at the forefront of the medical profession and other examined professions, but its social position, expressed by objective measures (earnings, structural possibilities, social power), is significantly lower.
Cross-Cultural Adaptation and Evaluation of the Participation and Environment Measure for Children and Youth to the Indian Context—A Mixed-Methods Study
Authors: Srinivasan Roopa , Kulkarni Vrushali , Smriti Sana , Teplicky Rachel , Anaby Dana
Published: February 2021

Culturally appropriate measures enable knowledge transfer and quality improvement of rehabilitation services in diverse contexts. The Applied Cultural Equivalence Framework (ACEF) was used in a two-phased mixed methods study to adapt and evaluate the Participation and Environment Measure-Children and Youth (PEM-CY) in India. Cognitive interviews with caregivers of children with disabilities (n = 15) aged 5–17 years established conceptual, item, semantic, and operational equivalence of the Indian PEM-CY. Construct validity was assessed by comparing PEM-CY scores of children with and without disabilities (n = 130) using a case-control design. Cognitive interviews resulted in operational (60.3%), semantic (26.4%), and item-level (13.2%) modifications in the PEM-CY with no changes at the conceptual level. Internal consistency (n = 130) was acceptable to excellent (0.61–0.87) on most scales. Test–retest reliability (n = 30) was good to excellent (ICC ≥ 0.75, Kappa 0.6–1.0) for most scales. Significant differences in all PEM-CY summary scores were found between children with and without disabilities, except for environmental supports. Children with disabilities had lower scores on frequency and involvement in activities across all settings; their caregivers desired greater change in participation and reported experiencing more environmental barriers across settings. Findings suggest the adapted PEM-CY is a valid and reliable measure for assessing the participation of Indian children.
Tumor Accumulation and Off-Target Biodistribution of an Indocyanine-Green Fluorescent Nanotracer: An Ex Vivo Study on an Orthotopic Murine Model of Breast Cancer
Authors: Sevieri Marta , Sitia Leopoldo , Bonizzi Arianna , Truffi Marta , Mazzucchelli Serena , Corsi Fabio
Published: February 2021

Indocyanine green (ICG) is a near infrared fluorescent tracer used in image-guided surgery to assist surgeons during resection. Despite appearing as a very promising tool for surgical oncology, its employment in this area is limited to lymph node mapping or to laparoscopic surgery, as it lacks tumor targeting specificity. Recently, a nanoformulation of this dye has been proposed with the aim toward tumor targeting specificity in order to expand its employment in surgical oncology. This nanosystem is constituted by 24 monomers of H-Ferritin (HFn), which self-assemble into a spherical cage structure enclosing the indocyanine green fluorescent tracer. These HFn nanocages were demonstrated to display tumor homing due to the specific interaction between the HFn nanocage and transferrin receptor 1, which is overexpressed in most tumor tissues. Here, we provide an ex vivo detailed comparison between the biodistribution of this nanotracer and free ICG, combining the results obtained with the Karl Storz endoscope that is currently used in clinical practice and the quantification of the ICG signal derived from the fluorescence imaging system IVIS Lumina II. These insights demonstrate the suitability of this novel HFn-based nanosystem in fluorescence-guided oncological surgery.
CD200:CD200R Interactions and Their Importance in Immunoregulation
Authors: Kotwica-Mojzych Katarzyna , Jodłowska-Jędrych Barbara , Mojzych Mariusz
Published: February 2021

The molecule CD200, described many years ago as a naturally occurring immunomodulatory agent, capable of regulating inflammation and transplant rejection, has attracted additional interest over the past years with the realization that it may also serve as an important marker for progressive malignancy. A large body of evidence also supports the hypothesis that this molecule can contribute to immunoregulation of, among other diseases, infection, autoimmune disease and allergy. New data have also come to light to characterize the receptors for CD200 (CD200R) and their potential mechanism(s) of action at the biochemical level, as well as the description of a novel natural antagonist of CD200, lacking the NH2-terminal region of the full-length molecule. Significant controversies exist concerning the relative importance of CD200 as a ligand for all reported CD200Rs. Nevertheless, some progress has been made in the identification of the structural constraints determining the interaction between CD200 and CD200R, and this information has in turn proved of use in developing novel small molecule agonists/antagonists of the interaction. The review below highlights many of these newer findings, and attempts to place them in the broad context of our understanding of the role of CD200-CD200R interactions in a variety of human diseases.
FNDC5/Irisin System in Neuroinflammation and Neurodegenerative Diseases: Update and Novel Perspective
Authors: Pignataro Patrizia , Dicarlo Manuela , Zerlotin Roberta , Zecca Chiara , Dell’Abate Maria Teresa , Buccoliero Cinzia , Logroscino Giancarlo , Colucci Silvia , Grano Maria
Published: February 2021

Irisin, the circulating peptide originating from fibronectin type III domain-containing protein 5 (FNDC5), is mainly expressed by muscle fibers under peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) control during exercise. In addition to several beneficial effects on health, physical activity positively affects nervous system functioning, particularly the hippocampus, resulting in amelioration of cognition impairments. Recently, FNDC5/irisin detection in hippocampal neurons and the presence of irisin in the cerebrospinal fluid opened a new intriguing chapter in irisin history. Interestingly, in the hippocampus of mice, exercise increases FNDC5 levels and upregulates brain-derived neurotrophic factor (BDNF) expression. BDNF, displaying neuroprotection and anti-inflammatory effects, is mainly produced by microglia and astrocytes. In this review, we discuss how these glial cells can morphologically and functionally switch during neuroinflammation by modulating the expression of a plethora of neuroprotective or neurotoxic factors. We also focus on studies investigating the irisin role in neurodegenerative diseases (ND). The emerging involvement of irisin as a mediator of the multiple positive effects of exercise on the brain needs further studies to better deepen this issue and the potential use in therapeutic approaches for neuroinflammation and ND.
Chronic Treatment of Ascorbic Acid Leads to Age-Dependent Neuroprotection against Oxidative Injury in Hippocampal Slice Cultures
Authors: Lee Kyung Hee , Kim Un Jeng , Cha Myeounghoon , Lee Bae Hwan
Published: February 2021

Increased oxidative damage in the brain, which increases with age, is the cause of abnormal brain function and various diseases. Ascorbic acid (AA) is known as an endogenous antioxidant that provides neuronal protection against oxidative damage. However, with aging, its extracellular concentrations and uptake decrease in the brain. Few studies have dealt with age-related functional changes in the brain to sustained ascorbate supplementation. This study aimed to investigate the susceptibility of hippocampal neurons to oxidative injury following acute and chronic AA administration. Oxidative stress was induced by kainic acid (KA, 5 µM) for 18 h in hippocampal slice cultures. After KA exposure, less neuronal cell death was observed in the 3 w cultured slice compared to the 9 w cultured slice. In the chronic AA treatment (6 w), the 9 w-daily group showed reduced neuronal cell death and increased superoxide dismutase (SOD) and Nrf2 expressions compared to the 9 w. In addition, the 9 w group showed delayed latencies and reduced signal activity compared to the 3 w, while the 9 w-daily group showed shorter latencies and increased signal activity than the 9 w. These results suggest that the maintenance of the antioxidant system by chronic AA treatment during aging could preserve redox capacity to protect hippocampal neurons from age-related oxidative stress.
Spermatozoa Transcriptional Response and Alterations in PL Proteins Properties after Exposure of Mytilus galloprovincialis to Mercury
Authors: Lettieri Gennaro , Notariale Rosaria , Ambrosino Alessia , Di Bonito Alfredo , Giarra Antonella , Trifuoggi Marco , Manna Caterina , Piscopo Marina
Published: February 2021

Mercury (Hg) is an environmental pollutant that impacts human and ecosystem health. In our previous works, we reported alterations in the properties of Mytilus galloprovincialis protamine-like (PL) proteins after 24 h of exposure to subtoxic doses of toxic metals such as copper and cadmium. The present work aims to assess the effects of 24 h of exposure to 1, 10, and 100 pM HgCl2 on spermatozoa and PL proteins of Mytilus galloprovincialis. Inductively coupled plasma–mass spectrometry indicated accumulation of this metal in the gonads of exposed mussels. Further, RT-qPCR analyses showed altered expression levels of spermatozoa mt10 and hsp70 genes. In Mytilus galloprovincialis, PL proteins represent the major basic component of sperm chromatin. These proteins, following exposure of mussels to HgCl2, appeared, by SDS-PAGE, partly as aggregates and showed a decreased DNA-binding capacity that rendered them unable to prevent DNA damage, in the presence of CuCl2 and H2O2. These results demonstrate that even these doses of HgCl2 exposure could affect the properties of PL proteins and result in adverse effects on the reproductive system of this organism. These analyses could be useful in developing rapid and efficient chromatin-based genotoxicity assays for pollution biomonitoring programs.
Durability of Gerber Saddles in RC Bridges: Analyses and Applications (Musmeci Bridge, Italy)
Authors: Santarsiero Giuseppe , Masi Angelo , Picciano Valentina
Published: February 2021

Guaranteeing adequate safety levels in critical infrastructures such as bridges is essential to modern societies and their vital services. Bridges with reinforced concrete structures are subject to deterioration, especially due to corrosion effects. Gerber saddles are among the key components of bridges which are especially exposed to environmental actions due to their position and reduced possibility of inspection. In this paper, a framework for the durability analysis of these components is proposed, considering the simultaneous presence of permanent loads and environmental actions under the form of chloride ions. Nonlinear numerical simulations adopting the finite element code ATENA are performed, accounting for chloride ingress analyses. The presence of cracks (due to applied loads and/or design/construction defects) which may speed-up corrosion propagation, steel reinforcement loss, cracking and spalling, and their effects on the load-bearing capacity is considered. This framework has been applied to the Gerber saddles of a prominent reinforced concrete (RC) bridge, namely the Musmeci bridge in Potenza, Italy. Durability analyses made it possible to evaluate the saddles’ strength capacity (i) at the time of construction, (ii) after forty-five years since the construction, and (iii) at an extended time of fifty years. The results show that corrosion can influence both the ultimate load capacity and the collapse mechanism.
Enhancement in Interply Toughness of BMI Composites Using Micro-Thin Films
Authors: Mathew Eldho , Joshi Sunil Chandrakant , Manikandan Periyasamy
Published: February 2021

Nowadays, laminated composites are widely used in the aerospace sector. All laminates have interply/interlaminar interfaces even if they are made using automated processes. The interfaces act as the areas of weaknesses and the potential crack initiation regions. Hence, any enhancement in the crack initiation and propagation resistance is always sought after. Usage of polymeric thin films is one of the promising and viable ways to achieve this. It is also easy to incorporate micro-thin films into any automation process. In the present study, different customized thin films that are compatible with Glass/BMI composites are fabricated. Fracture toughness tests in Mode I (opening mode), Mode II (sliding mode) and Mixed Mode I/II are conducted respectively using Double Cantilever Beam (DCB), End Notch Flexure (ENF) and Mixed Mode Bending (MMB) test specimens. This paper discusses the manufacturing of compatible micro-thin films. The various challenges faced during the manufacturing and incorporation of thin films are presented. The results of the various fracture toughness tests are examined. Mechanisms through which the different films help in resisting the crack initiation and propagation are deliberated and discussed. The incorporation of this technique in Automated Fiber Placement (AFP) is also discussed.
Coral Image Segmentation with Point-Supervision via Latent Dirichlet Allocation with Spatial Coherence
Authors: Yu Xi , Ouyang Bing , Principe Jose C.
Published: February 2021

Deep neural networks provide remarkable performances on supervised learning tasks with extensive collections of labeled data. However, creating such large well-annotated data sets requires a considerable amount of resources, time and effort, especially for underwater images data sets such as corals and marine animals. Therefore, the overreliance on labels is one of the main obstacles for widespread applications of deep learning methods. In order to overcome this need for large annotated dataset, this paper proposes a label-efficient deep learning framework for image segmentation using only very sparse point-supervision. Our approach employs a latent Dirichlet allocation (LDA) with spatial coherence on feature space to iteratively generate pseudo labels. The method requires, as an initial condition, a Wide Residual Network (WRN) trained with sparse labels and mutual information constraints. The proposed method is evaluated on the sparsely labeled coral image data set collected from the Pulley Ridge region in the Gulf of Mexico. Experiments show that our method can improve image segmentation performance against sparsely labeled samples and achieves better results compared with other semi-supervised approaches.
Investigation of the Starting-Up Axial Hydraulic Force and Structure Characteristics of Pump Turbine in Pump Mode
Authors: Mao Zhongyu , Tao Ran , Chen Funan , Bi Huili , Cao Jingwei , Luo Yongyao , Fan Honggang , Wang Zhengwei
Published: February 2021

During the starting up of the pump mode in pump turbines, the axial hydraulic force acting on the runner would develop with the guide vane opening. It causes deformation and stress on the support bracket, main shaft and runner, which influence the operation security. In this case, the axial hydraulic force of the pump turbine is studied during the starting up of pump mode. Its influences on the support bracket and main shaft are investigated in detail. Based on the prediction results of axial hydraulic force, the starting-up process can be divided into “unsteady region” and “Q flat region” with obviously different features. The mechanism is also discussed by analyzing pressure distributions and streamlines. The deformation of the support bracket and main shaft are found to have a relationship with the resultant force on the crown and band. A deflection is found on the deformation of the runner with the nodal diameter as the midline in the later stages of the starting-up process. The reason is discussed according to pressure distributions. The stress concentration of the support bracket is found on the connection between thrust seating and support plates. The stress of the runner is mainly on the connection between the crown and the blade’s leading-edge. This work will provide more useful information and strong references for similar cases. It will also help in the design of pump turbine units with more stabilized systems for reducing over-loaded hydraulic force, and in the solving of problems related to structural characteristics.
Pain Standards for Accredited Healthcare Organizations (ACDON Project): A Mixed Methods Study
Authors: Pérez Concepción , Martin-Delgado Jimmy , Vinuesa Mercedes , Ibor Pedro J. , Guilabert Mercedes , Gomez José , Beato Carmen , Sánchez-Jiménez Juana , Velázquez Ignacio , Calvo-Espinos Claudio , Cánovas María L. , Yáñez José A. , Rodríguez Mireia , Baquero José L. , Gallach Elisa , Folch Emma , Tuca Albert , Santiña Manel , Mira José J.
Published: February 2021

Up to 50% of cancer patients and up to 90% of those in terminal stages experience pain associated with disease progression, poor quality of life, and social impact on caregivers. This study aimed to establish standards for the accreditation of oncological pain management in healthcare organizations. A mixed methods approach was used. First, a pragmatic literature review was conducted. Second, consensus between professionals and patients was reached using the Nominal Group and Delphi technique in a step that involved anesthesiologists, oncologists, family physicians, nurses, psychologists, patient representatives, and caregivers. Third, eight hospitals participated in a pilot assessment of the level of fulfillment of each standard. A total of 37 standards were extracted. The Nominal Group produced additional standards, of which 60 were included in Questionnaire 0 that was used in the Delphi Technique. Two Delphi voting rounds were performed to reach a high level of consensus, and involved 64 and 62 participants with response rates of 90% and 87%, respectively. Finally, 39 standards for the management of cancer pain were agreed upon. In the self-evaluation, the average range of compliance was between 56.4% and 100%. The consensus standards of the ACDON Project might improve the monitoring of cancer pain management. These standards satisfied the demands of professionals and patients and could be used for the accreditation of approaches in cancer pain management.
The Endothelium as a Target for Anti-Atherogenic Therapy: A Focus on the Epigenetic Enzymes EZH2 and SIRT1
Authors: Fledderus Jolien , Vanchin Byambasuren , Rots Marianne , Krenning Guido
Published: February 2021

Endothelial cell inflammatory activation and dysfunction are key events in the pathophysiology of atherosclerosis, and are associated with an elevated risk of cardiovascular events. Yet, therapies specifically targeting the endothelium and atherosclerosis are lacking. Here, we review how endothelial behaviour affects atherogenesis and pose that the endothelium may be an efficacious cellular target for antiatherogenic therapies. We discuss the contribution of endothelial inflammatory activation and dysfunction to atherogenesis and postulate that the dysregulation of specific epigenetic enzymes, EZH2 and SIRT1, aggravate endothelial dysfunction in a pleiotropic fashion. Moreover, we propose that commercially available drugs are available to clinically explore this postulation.
Cardioprotective Effect of Linalool against Isoproterenol-Induced Myocardial Infarction
Authors: Mohamed Maged E. , Abduldaium Mohamed S. , Younis Nancy S.
Published: February 2021

Background: Myocardial infarction (MI), a life-threatening disorder, arises from the imbalance between oxygen supply and myocardial demand. Linalool is a naturally occurring monoterpenes with proved numerous pharmacological actions. This study investigated the cardioprotective effect of Linalool on isoproterenol (ISO)-induced MI in rat models and explored part of the underlying molecular mechanisms. Methods: Rats were divided into five groups; groups I and II served as normal and linalool control groups, Group III administered ISO alone; groups V and VI received two different doses of Linalool and were challenged by ISO. Different biochemical parameters were determined, including hemodynamic, infarction size, cardiac enzymes, apoptotic markers, and inflammatory mediators. Results: Linalool limited the infarcted area size and diminished the elevated cardiac enzymes. Linalool escalated HO-1 and Nrf2, both nuclear and cytosol fractions, and reduced Keap 1. Linalool enhanced cardiac antioxidant activities, reduced inflammatory cytokines (tumor necrosis factor-alpha (TNF-α), nuclear factor-κ-B (NF-κB), interleukin 1 beta (IL-1β), interleukin 6 (IL-6)), apoptotic markers (Caspase-3, Caspase-9, and Bax), and elevated Bcl2. Conclusion: Linalool could act as an effective cardioprotective agent in the MI model through improving the oxidative condition, probably via the Nrf2/HO-1 pathway and by abolishing both apoptotic and inflammatory responses.
Machine Learning Use for Prognostic Purposes in Multiple Sclerosis
Authors: Seccia Ruggiero , Romano Silvia , Salvetti Marco , Crisanti Andrea , Palagi Laura , Grassi Francesca
Published: February 2021

The course of multiple sclerosis begins with a relapsing-remitting phase, which evolves into a secondarily progressive form over an extremely variable period, depending on many factors, each with a subtle influence. To date, no prognostic factors or risk score have been validated to predict disease course in single individuals. This is increasingly frustrating, since several treatments can prevent relapses and slow progression, even for a long time, although the possible adverse effects are relevant, in particular for the more effective drugs. An early prediction of disease course would allow differentiation of the treatment based on the expected aggressiveness of the disease, reserving high-impact therapies for patients at greater risk. To increase prognostic capacity, approaches based on machine learning (ML) algorithms are being attempted, given the failure of other approaches. Here we review recent studies that have used clinical data, alone or with other types of data, to derive prognostic models. Several algorithms that have been used and compared are described. Although no study has proposed a clinically usable model, knowledge is building up and in the future strong tools are likely to emerge.
Animal Coronaviruses and SARS-COV-2 in Animals, What Do We Actually Know?
Authors: Bonilauri Paolo , Rugna Gianluca
Published: February 2021

Coronaviruses (CoVs) are a well-known group of viruses in veterinary medicine. We currently know four genera of Coronavirus, alfa, beta, gamma, and delta. Wild, farmed, and pet animals are infected with CoVs belonging to all four genera. Seven human respiratory coronaviruses have still been identified, four of which cause upper-respiratory-tract diseases, specifically, the common cold, and the last three that have emerged cause severe acute respiratory syndromes, SARS-CoV-1, MERS-CoV, and SARS-CoV-2. In this review we briefly describe animal coronaviruses and what we actually know about SARS-CoV-2 infection in farm and domestic animals.
Fatigue Failure from Inner Surfaces of Additive Manufactured Ti-6Al-4V Components
Authors: de Jesus Joel , Martins Ferreira José António , Borrego Luís , Costa José D. , Capela Carlos
Published: February 2021

Selective laser melting (SLM) is an additive manufacturing process for producing metallic components with complex geometries. A drawback of this process is the process-inherent poor surface finish, which is highly detrimental in materials submitted to fatigue loading situations. The goal of this work is to analyze the fatigue behavior of Ti-6Al-4V specimens with internal axial channels under the following different conditions: hole drilled, hole as manufactured, and hole threaded M4 × 0.7. All the cases studied showed a lower fatigue performance as compared with solid samples due to the surface roughness and geometry effect that produced a surface stress concentration leading to a reduction in fatigue strength. The fractography revealed that crack initiation occurred from the internal surface in all specimens with internal channel mostly from defects as unfused particles and lack of fusion zones, while for the solid specimens crack initiation was observed from the external surface due to insufficient fusion defect. The application of the Smith-Watson-Topper energy-based parameter was revealed to be a good tool for fatigue life prediction of the different series studied.