bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel t(8;14)(q24;q11) Rearranged Human Cell Line as a Model for Mechanistic and Drug Discovery Studies of NOTCH1-Independent Human T-Cell Leukemia
Authors: Tosello Valeria , Milani Gloria , Martines Annalisa , Macri Nadia , Van Loocke Wouder , Matthijssens Filip , Buldini Barbara , Minuzzo Sonia , Bongiovanni Deborah , Schumacher Richard Fabian , Amadori Alberto , Van Vlierberghe Pieter , Piovan Erich
Published: October 2018

MYC-translocated T-lineage acute lymphoblastic leukemia (T-ALL) is a rare subgroup of T-ALL associated with CDKN2A/B deletions, PTEN inactivation, and absence of NOTCH1 or FBXW7 mutations. This subtype of T-ALL has been associated with induction failure and aggressive disease. Identification of drug targets and mechanistic insights for this disease are still limited. Here, we established a human NOTCH1-independent MYC-translocated T-ALL cell line that maintains the genetic and phenotypic characteristics of the parental leukemic clone at diagnosis. The University of Padua T-cell acute lymphoblastic leukemia 13 (UP-ALL13) cell line has all the main features of the above described MYC-translocated T-ALL. Interestingly, UP-ALL13 was found to harbor a heterozygous R882H DNMT3A mutation typically found in myeloid leukemia. Chromatin immunoprecipitation coupled with high-throughput sequencing for histone H3 lysine 27 (H3K27) acetylation revealed numerous putative super-enhancers near key transcription factors, including MYC, MYB, and LEF1. Marked cytotoxicity was found following bromodomain-containing protein 4 (BRD4) inhibition with AZD5153, suggesting a strict dependency of this particular subtype of T-ALL on the activity of super-enhancers. Altogether, this cell line may be a useful model system for dissecting the signaling pathways implicated in NOTCH1-independent T-ALL and for the screening of targeted anti-leukemia agents specific for this T-ALL subgroup.
Magnetic Properties and Microstructure of FePt(BN, Ag, C) Films
Authors: Tsai Jai-Lin , Chen Yu-Ren , Chen Jyun-You , Hsu Ting-Wei , Dai Cheng , Hsu Chia-Jen
Published: October 2018

The microstructure and magnetic properties of FePt(BN, Ag, C) granular films grown on the MgTiON intermediate layer with and without the MoC inserting layer were studied. Without the MoC inserting layer, the 6 nm thick FePt film is continuous, which favors the domain wall motion magnetization reversal process and shows a lower out-of-plane coercivity (Hc) value of 6.7 kOe. The FePt(BN, Ag, C) granular film was grown in ball- and square-like grains with an almost vertical contact angle, and the out-of-plane coercivity (Hc) was increased to 15.5 kOe. When the MoC with a thickness of 3 nm was capped on the MgTiON intermediate layer, the FePt grains with and without (BN, Ag, C) segregants were both formed in large trapezoidal islands with a low contact angle morphology. The out-of-plane Hc value changed from 14.9 to 13.2 kOe and the reduced coercivity was due to larger grain sizes and a lower ordering degree of the FePt(BN, Ag, C) film.
Low Power Robust Early Output Asynchronous Block Carry Lookahead Adder with Redundant Carry Logic
Authors: Balasubramanian Padmanabhan , Maskell Douglas , Mastorakis Nikos
Published: October 2018

Adder is an important datapath unit of a general-purpose microprocessor or a digital signal processor. In the nanoelectronics era, the design of an adder that is modular and which can withstand variations in process, voltage and temperature are of interest. In this context, this article presents a new robust early output asynchronous block carry lookahead adder (BCLA) with redundant carry logic (BCLARC) that has a reduced power-cycle time product (PCTP) and is a low power design. The proposed asynchronous BCLARC is implemented using the delay-insensitive dual-rail code and adheres to the 4-phase return-to-zero (RTZ) and the 4-phase return-to-one (RTO) handshaking. Many existing asynchronous ripple-carry adders (RCAs), carry lookahead adders (CLAs) and carry select adders (CSLAs) were implemented alongside to perform a comparison based on a 32/28 nm complementary metal-oxide-semiconductor (CMOS) technology. The 32-bit addition was considered for an example. For implementation using the delay-insensitive dual-rail code and subject to the 4-phase RTZ handshaking (4-phase RTO handshaking), the proposed BCLARC which is robust and of early output type achieves: (i) 8% (5.7%) reduction in PCTP compared to the optimum RCA, (ii) 14.9% (15.5%) reduction in PCTP compared to the optimum BCLARC, and (iii) 26% (25.5%) reduction in PCTP compared to the optimum CSLA.
Agile Service Engineering in the Industrial Internet of Things
Authors: Usländer Thomas , Batz Thomas
Published: October 2018

The emerging Industrial Internet of Things (IIoT) will not only leverage new and potentially disruptive business models but will also change the way software applications will be analyzed and designed. Agility is a need in a systematic service engineering as well as a co-design of requirements and architectural artefacts. Functional and non-functional requirements of IT users (in smart manufacturing mostly from the disciplines of mechanical engineering and electrical engineering) need to be mapped to the capabilities and interaction patterns of emerging IIoT service platforms, not to forget the corresponding information models. The capabilities of such platforms are usually described, structured, and formalized by software architects and software engineers. However, their technical descriptions are far away from the thinking and the thematic terms of end-users. This complicates the transition from requirements analysis to system design, and hence the re-use of existing and the design of future platform capabilities. Current software engineering methodologies do not systematically cover these interlinked and two-sided aspects. The article describes in a comprehensive manner how to close this gap with the help of a service-oriented analysis and design methodology entitled SERVUS (also mentioned in ISO 19119 Annex D) and a corresponding Web-based Platform Engineering Information System (PEIS).
Learning to Set the Reserve Price Optimally in Laboratory First Price Auctions
Authors: Banerjee Priyodorshi , Khare Shashwat , Srikant P.
Published: October 2018

We analyze choices of sellers, each setting a reserve price in a laboratory first price auction with automated equilibrium bidding. Subjects are allowed to gain experience for a fixed period of time prior to making a single payoff-relevant choice. Behavior of more experienced sellers was consistent with benchmark theory: average reserve price for these sellers was independent of the number of bidders and equaled the predicted level. Less experienced sellers however deviated from the theoretical benchmark: on average, they tended to shade reserve price below the predicted level and positively relate it to the number of bidders.
“Out of the Can”: A Draft Genome Assembly, Liver Transcriptome, and Nutrigenomics of the European Sardine, Sardina pilchardus
Authors: Machado André M. , Tørresen Ole K. , Kabeya Naoki , Couto Alvarina , Petersen Bent , Felício Mónica , Campos Paula F. , Fonseca Elza , Bandarra Narcisa , Lopes-Marques Mónica , Ferraz Renato , Ruivo Raquel , Fonseca Miguel M. , Jentoft Sissel , Monroig Óscar , Da Fonseca Rute R. , C. Castro L. Filipe
Published: October 2018

Clupeiformes, such as sardines and herrings, represent an important share of worldwide fisheries. Among those, the European sardine (Sardina pilchardus, Walbaum 1792) exhibits significant commercial relevance. While the last decade showed a steady and sharp decline in capture levels, recent advances in culture husbandry represent promising research avenues. Yet, the complete absence of genomic resources from sardine imposes a severe bottleneck to understand its physiological and ecological requirements. We generated 69 Gbp of paired-end reads using Illumina HiSeq X Ten and assembled a draft genome assembly with an N50 scaffold length of 25,579 bp and BUSCO completeness of 82.1% (Actinopterygii). The estimated size of the genome ranges between 655 and 850 Mb. Additionally, we generated a relatively high-level liver transcriptome. To deliver a proof of principle of the value of this dataset, we established the presence and function of enzymes (Elovl2, Elovl5, and Fads2) that have pivotal roles in the biosynthesis of long chain polyunsaturated fatty acids, essential nutrients particularly abundant in oily fish such as sardines. Our study provides the first omics dataset from a valuable economic marine teleost species, the European sardine, representing an essential resource for their effective conservation, management, and sustainable exploitation.
Hard Ground-Soft Politics: The Biennial of Graphic Arts in Ljubljana and Biting of the Iron Curtain
Authors: Komorowski Wiktor
Published: October 2018

In 1955, the Ljubljana Biennial of Graphic Arts was founded. It was the first curatorial initiative that aimed to link graphic artists working around the world and with those divided by the Cold War. The Ljubljana Biennial became a major success and its model quickly spread worldwide, augmenting the international circulation of prints and exchanges of artistic concepts. Over the next twenty years similar exhibitions were established in Krakow, Tallinn, San Juan, Santiago de Chile, Cali, Tokyo, Cairo, Fredrikstad, Frechen, Sofia and Bradford. The Ljubljana Biennial of Graphic Arts offered an opportunity for artists from Yugoslavia, Eastern Europe and Latin America, such as Andrzej Lachowicz, Mauricio Leib Lasansky, Adolfo Quinteros and Aleš Veselý, to exhibit their works alongside the protagonists of the western contemporary graphic art circuit such as Robert Rauschenberg, Antonio Segui, Yozo Hamaguchi, Max Bill and Victor Vasarely. The network of exhibitions that followed the example set by Ljubljana Biennial of Graphic Arts became a window into the world not only for printmakers, but also for a number of artists who were affected by Cold War cultural exclusion. The network of dedicated international print exhibitions created favourable conditions for an emerging third space, which became a platform for communication between the cultures divided by the Iron Curtain. This article focuses on the curatorial assumptions that brought the Biennial of Graphic Arts in Ljubljana to life and questions its position as a cultural cornerstone for the Non-Aligned geopolitical order.
Strengthening the Brain—Is Resistance Training with Blood Flow Restriction an Effective Strategy for Cognitive Improvement?
Authors: Törpel Alexander , Herold Fabian , Hamacher Dennis , Müller Notger G. , Schega Lutz
Published: October 2018

Aging is accompanied by a decrease in physical capabilities (e.g., strength loss) and cognitive decline. The observed bidirectional relationship between physical activity and brain health suggests that physical activities could be beneficial to maintain and improve brain functioning (e.g., cognitive performance). However, the exercise type (e.g., resistance training, endurance training) and their exercise variables (e.g., load, duration, frequency) for an effective physical activity that optimally enhance cognitive performance are still unknown. There is growing evidence that resistance training induces substantial brain changes which contribute to improved cognitive functions. A relative new method in the field of resistance training is blood flow restriction training (BFR). While resistance training with BFR is widely studied in the context of muscular performance, this training strategy also induces an activation of signaling pathways associated with neuroplasticity and cognitive functions. Based on this, it seems reasonable to hypothesize that resistance training with BFR is a promising new strategy to boost the effectiveness of resistance training interventions regarding cognitive performance. To support our hypothesis, we provide rationales of possible adaptation processes induced by resistance training with BFR. Furthermore, we outline recommendations for future studies planning to investigate the effects of resistance training with BFR on cognition.
The CHA2DS2-VASc Score Predicts Major Bleeding in Non-Valvular Atrial Fibrillation Patients Who Take Oral Anticoagulants
Authors: Lee Kuang-Tso , Chang Shang-Hung , Yeh Yung-Hsin , Tu Hui-Tzu , Chan Yi-Hsin , Kuo Chi-Tai , See Lai-Chu
Published: October 2018

Background: Patients with atrial fibrillation (AF) are at a substantial risk of ischemic stroke. The CHA2DS2-VASc score predicts the risk of thromboembolism, but its role in predicting major bleeding in patients taking oral anticoagulants is unclear. Methods: We used the National Health Insurance Research Database (NHIRD) of Taiwan to identify patients with AF from 2010 to 2016. They were divided into four groups according to the oral anticoagulants. The outcomes were ischemic stroke/systemic thromboembolism, and major bleeding. Results: A total of 279,776 patients were identified. Ischemic stroke or systemic embolism events were observed in 1.73%, 3.62%, 4.36%, and 5.02% of the patients in the apixaban, rivaroxaban, dabigatran, and warfarin groups, respectively. Major bleeding was recorded in 1.18%, 2.66%, 3.23%, and 4.70% of the patients in the apixaban, rivaroxaban, dabigatran, and warfarin groups, respectively. The highest rates for both ischemic stroke and bleeding events occurred in the patients with a CHA2DS2-VASc score of five or more. Conclusion: Non-valvular AF patients with high CHA2DS2-VASc scores are susceptible to both systemic thromboembolism and major bleeding. The trend was consistently observed in patients who took non-vitamin K oral anticoagulants (NOACs) or warfarin. NOACs might be potentially more effective in reducing overall events.
Fluorescence Studies of the Interplay between Metal-Enhanced Fluorescence and Graphene-Induced Quenching
Authors: Wiwatowski Kamil , Podlas Paweł , Twardowska Magdalena , Maćkowski Sebastian
Published: October 2018

Fluorescence microscopy and spectroscopy were applied for studying the optical properties of a hybrid nanostructure, in which we combine plasmon-induced metal enhanced fluorescence with energy transfer to epitaxial graphene. Covering the layer of silver islands with a monolayer graphene, while turning on the efficient energy transfer from emitters, only moderately affects the enhancement of fluorescence attributed to the plasmon resonance in metallic nanostructures—as evidenced by the analysis of fluorescence decays. The results show that it is feasible to combine the properties of graphene with metal-enhanced fluorescence. The importance of the layer thickness of the emitters is also pointed out.
Analytical Micromechanics Models for Elastoplastic Behavior of Long Fibrous Composites: A Critical Review and Comparative Study
Authors: Wang Yanchao , Huang ZhengMing
Published: October 2018

Elasto-plastic models for composites can be classified into three categories in terms of a length scale, i.e., macro scale, meso scale, and micro scale (micromechanics) models. In general, a so-called multi-scale model is a combination of those at various length scales with a micromechanics one as the foundation. In this paper, a critical review is made for the elastoplastic models at the micro scale, and a comparative study is carried out on most popular analytical micromechanics models for the elastoplastic behavior of long fibrous composites subjected to a static load, meaning that creep and dynamic response are not concerned. Each model has been developed essentially following three steps, i.e., an elastic homogenization, a rule to define the yielding of a constituent phase, and a linearization for the elastoplastic response. The comparison is made for all of the three aspects. Effects of other issues, such as the stress field fluctuation induced by a high contrast heterogeneity, the stress concentration factors in the matrix, and the different approaches to a plastic Eshelby tensor, are addressed as well. Correlation of the predictions by different models with available experimental data is shown.
Chitosan-Based In Situ Gels for Ocular Delivery of Therapeutics: A State-of-the-Art Review
Authors: Irimia Teodora , Dinu-Pîrvu Cristina-Elena , Ghica Mihaela Violeta , Lupuleasa Dumitru , Muntean Daniela-Lucia , Udeanu Denisa Ioana , Popa Lăcrămioara
Published: October 2018

Ocular in situ gels are a promising alternative to overcome drawbacks of conventional eye drops because they associate the advantages of solutions such as accuracy and reproducibility of dosing, or ease of administration with prolonged contact time of ointments. Chitosan is a natural polymer suitable for use in ophthalmic formulations due to its biocompatibility, biodegradability, mucoadhesive character, antibacterial and antifungal properties, permeation enhancement and corneal wound healing effects. The combination of chitosan, pH-sensitive polymer, with other stimuli-responsive polymers leads to increased mechanical strength of formulations and an improved therapeutic effect due to prolonged ocular contact time. This review describes in situ gelling systems resulting from the association of chitosan with various stimuli-responsive polymers with emphasis on the mechanism of gel formation and application in ophthalmology. It also comprises the main techniques for evaluation of chitosan in situ gels, along with requirements of safety and ocular tolerability.
Anodic Bubble Behavior in a Laboratory Scale Transparent Electrolytic Cell for Aluminum Electrolysis
Authors: Huang Yipeng , Wang Zhaowen , Yang Youjian , Gao Bingliang , Shi Zhongning , Hu Xianwei
Published: October 2018

In the Hall-Héroult process for extracting aluminum, the evolution and dynamics of anodic bubbles have a significant influence on the efficiency of the overall electrolysis process. In this study, the behavior of the bubbles beneath the carbon anode in cryolite-alumina molten salt was studied for the first time using a laboratory-scale transparent electrolysis cell to view the anode from the bottom. The bubble dynamics and the relevant characteristic parameters of bubbles were obtained using video cameras and image processing. It was found that the bubbles were observed to preferentially generate at several areas on the underside of the anode and the morphologies of coalesced bubbles show excellent similarity. Moreover, the behavior of gas on carbon and graphite anodes was significantly different, where the carbon anode favored the forming of larger bubbles. These observations confirmed different types of carbon anodes cause different bubble behavior. These findings are expected to be useful in optimizing the aluminum electrolysis process on an industrial scale.
Photolithographic Patterning of Cytop with Limited Contact Angle Degradation
Authors: Qiu Yalei , Yang Shu , Sheng Kuang
Published: October 2018

Cytop is a commercially available amorphous fluoropolymer with excellent characteristics including electric insulation, water and oil repellency, chemical resistance, and moisture-proof property, making it an attractive material as hydrophobic layers in electrowetting-on-dielectric (EWOD) devices. However, its highly hydrophobic surface makes it difficult for photoresists to be directly coated on the surface. To pattern Cytop, plasma treatment prior to applying photoresists is required to promote the adhesion between the photoresist and the Cytop coating. This approach inevitably causes hydrophobicity loss in the final EWOD devices. Thus, a damage-reduced recipe for Cytop patterning is urgently needed. In this paper, we first characterized the damage caused by two categories of surface treatment methods: plasma treatment and metal treatment. Parameters such as plasma gas source (Ar/O2), plasma treatment time (0–600 s), metal target (Al/Cu/Cr/Au), metal deposition process (magnetron sputtering or e-beam evaporation) were varied. Film thickness, wettability, and roughness were quantified by ellipsometry measurements, contact angle measurements, and atom force microscope (AFM), respectively. We then evaluated the effectiveness of annealing in damage reduction. Experimental results show that: (1) annealing is necessary in restoring hydrophobicity as well as smoothing surfaces; (2) specified film thickness can be obtained by controlling plasma treatment time; (3) “Ar/O2 plasma treatment + an AZ5214 soft mask + annealing” is a feasible recipe; (4) “an Al/Cu/Cr/Au hard mask + annealing” is feasible as well.
Rare Earth Elements Geochemistry and C–O Isotope Characteristics of Hydrothermal Calcites: Implications for Fluid-Rock Reaction and Ore-Forming Processes in the Phapon Gold Deposit, NW Laos
Authors: Guo Linnan , Hou Lin , Liu Shusheng , Nie Fei
Published: October 2018

The Phapon gold deposit is located in the northern Laos and the northern segments of the Luang Prabang–Loei metallogenic belt. The lode-gold orebodies consist of auriferous calcite veins in the middle, and the surrounding siderite alteration and hematite alteration zones in red color. This deposit is hosted in Lower Permian limestone and controlled by a NE-trending ductile–brittle fault system, and it is characterized by the wallrock alteration of carbonatization and lack of quartz and metal sulfides. The hydrothermal calcite from auriferous calcite veins and red alteration zone, as well as the wall rocks of limestone and sandstone were selected for rare earth elements (REE) and C–O isotope analyses. The two types of calcite and limestone have generally consistent REE patterns and δEu and δCe values, which are completely different from those of sandstone. Calcites from the auriferous vein show slight light rare earth elements (LREE)-depleted patterns and higher Tb/La and Sm/Nd ratios than the ones from the red alteration zone with slight LREE-enriched patterns. These values indicate that the calcites from the auriferous veins and the red alteration zones are products of homologous fluids, but the former ones are generally likely to form later than the latter ones. The hydrothermal calcites have C–O isotope compositions within the range of marine carbonate, and markedly different from the magmatic or mantle reservoir values. Taking the Y/Ho–La/Ho and Tb/Ca–Tb/La variations into consideration, we believe the hydrothermal calcites could be formed from remobilization and recrystallization of the ore-hosted limestone, and the fluid-wallrock interaction played a major role in the gold mineralization in Phapon. In combination with the regional and local geology, the ore-forming process is suspected to be primarily associated with dehydration and decarbonisation of the Lower Permian limestone and Middle–Upper Triassic sandstones. The Phapon gold deposit could have been formed during the Late Triassic–Jurassic regional dynamic metamorphism driven by Indochina–Sibumasu post-collisional magmatism. A number of features in Phapon are similar to epizonal orogenic deposit, but it is still a unique calcite vein type gold deposit in the Luang Prabang-Loei metallogenic belt.
Nanostructured Dihydroartemisinin Plus Epirubicin Liposomes Enhance Treatment Efficacy of Breast Cancer by Inducing Autophagy and Apoptosis
Authors: Hu Ying-Jie , Zhang Jing-Ying , Luo Qian , Xu Jia-Rui , Yan Yan , Mu Li-Min , Bai Jing , Lu Wan-Liang
Published: October 2018

The heterogeneity of breast cancer and the development of drug resistance are the relapse reasons of disease after chemotherapy. To address this issue, a combined therapeutic strategy was developed by building the nanostructured dihydroartemisinin plus epirubicin liposomes. Investigations were performed on human breast cancer cells in vitro and xenografts in nude mice. The results indicated that dihydroartemisinin could significantly enhance the efficacy of epirubicin in killing different breast cancer cells in vitro and in vivo. We found that the combined use of dihydroartemisinin with epirubicin could efficiently inhibit the activity of Bcl-2, facilitate release of Beclin 1, and further activate Bax. Besides, Bax activated apoptosis which led to the type I programmed death of breast cancer cells while Beclin 1 initiated the excessive autophagy that resulted in the type II programmed death of breast cancer cells. In addition, the nanostructured dihydroartemisinin plus epirubicin liposomes prolonged circulation of drugs, and were beneficial for simultaneously delivering drugs into breast cancer tissues. Hence, the nanostructured dihydroartemisinin plus epirubicin liposomes could provide a new therapeutic strategy for treatment of breast cancer.
Surface Modification of Magnetic Iron Oxide Nanoparticles
Authors: Zhu Nan , Ji Haining , Yu Peng , Niu Jiaqi , Farooq M. U. , Akram M. Waseem , Udego I. O. , Li Handong , Niu Xiaobin
Published: October 2018

Functionalized iron oxide nanoparticles (IONPs) are of great interest due to wide range applications, especially in nanomedicine. However, they face challenges preventing their further applications such as rapid agglomeration, oxidation, etc. Appropriate surface modification of IONPs can conquer these barriers with improved physicochemical properties. This review summarizes recent advances in the surface modification of IONPs with small organic molecules, polymers and inorganic materials. The preparation methods, mechanisms and applications of surface-modified IONPs with different materials are discussed. Finally, the technical barriers of IONPs and their limitations in practical applications are pointed out, and the development trends and prospects are discussed.
Alignment of Supermarket Own Brand Foods’ Front-of-Pack Nutrition Labelling with Measures of Nutritional Quality: An Australian Perspective
Authors: Pulker Claire Elizabeth , Trapp Georgina S. A. , Scott Jane Anne , Pollard Christina Mary
Published: October 2018

Two voluntary front-of-pack nutrition labels (FOPNL) are present in Australia: the government-led Health Star Ratings (HSR) and food industry-led Daily Intake Guide (DIG). Australia’s two largest supermarkets are key supporters of HSR, pledging uptake on all supermarket own brand foods (SOBF). This study aimed to examine prevalence of FOPNL on SOBF, and alignment with patterns of nutritional quality. Photographic audits of all SOBF present in three large supermarkets were conducted in Perth, Western Australia, in 2017. Foods were classified as nutritious or nutrient-poor based on the Australian Guide to Healthy Eating (AGTHE), NOVA level of food processing, and HSR score. Most (81.5%) SOBF featured FOPNL, with only 55.1% displaying HSR. HSR was present on 69.2% of Coles, 54.0% of Woolworths, and none of IGA SOBF. Half (51.3%) of SOBF were classified as nutritious using the AGTHE, but using NOVA, 56.9% were ultra-processed foods. Nutrient-poor and ultra-processed SOBF were more likely than nutritious foods to include HSR, yet many of these foods achieved HSR scores of 2.5 stars or above, implying they were a healthy choice. Supermarkets have a powerful position in the Australian food system, and they could do more to support healthy food selection through responsible FOPNL.
Selenium Analysis and Speciation in Dietary Supplements Based on Next-Generation Selenium Ingredients
Authors: Constantinescu-Aruxandei Diana , Frîncu Rodica Mihaela , Capră Luiza , Oancea Florin
Published: October 2018

Selenium is essential for humans and the deficit of Se requires supplementation. In addition to traditional forms such as Se salts, amino acids, or selenium-enriched yeast supplements, next-generation selenium supplements, with lower risk for excess supplementation, are emerging. These are based on selenium forms with lower toxicity, higher bioavailability, and controlled release, such as zerovalent selenium nanoparticles (SeNPs) and selenized polysaccharides (SPs). This article aims to focus on the existing analytical systems for the next-generation Se dietary supplement, providing, at the same time, an overview of the analytical methods available for the traditional forms. The next-generation dietary supplements are evaluated in comparison with the conventional/traditional ones, as well as the analysis and speciation methods that are suitable to reveal which Se forms and species are present in a dietary supplement. Knowledge gaps and further research potential in this field are highlighted. The review indicates that the methods of analysis of next-generation selenium supplements should include a step related to chemical species separation. Such a step would allow a proper characterization of the selenium forms/species, including molecular mass/dimension, and substantiates the marketing claims related to the main advantages of these new selenium ingredients.
Sugar-Sweetened Beverage Consumption in Relation to Obesity and Metabolic Syndrome among Korean Adults: A Cross-Sectional Study from the 2012–2016 Korean National Health and Nutrition Examination Survey (KNHANES)
Authors: Shin Sangah , Kim Seong-Ah , Ha Jinwoo , Lim Kyungjoon
Published: October 2018

It is well known that the consumption of sugar-sweetened beverages (SSBs) increases the risk of developing obesity and metabolic syndrome (MetS). However, there are not many studies investigating the link between SSBs and increased incidences of diseases in the Asian population, and in particular, in Korea. We explored the association of SSB consumption with the risk of developing obesity and MetS among Korean adults (12,112 participants from the 2012–2016 Korean National Health and Nutrition Examination Survey). We calculated the total SSB consumption frequency by counting each beverage item, including soda beverages, fruit juices, and sweetened rice drinks. Obesity was defined as a body mass index ≥25 kg/m2, and MetS was defined using the National Cholesterol Education Program, Adult Treatment Panel III. A survey logistic regression analyses was conducted to examine the association of SSB consumption with obesity and MetS, adjusting for related confounders such as age, energy intake, household income, education, alcohol drinking, smoking status, and physical activity. The SSB consumption was positively associated with an increased risk of the prevalence for obesity (Odd ratio (OR): 1.60; 95% confidence interval (CI): 1.23–2.09; p for trend = 0.0009) and MetS (OR: 1.61; 95% CI: 1.20–2.16; p for trend = 0.0003) among women. In men, SSB consumption only contributed to a higher prevalence of obesity (OR: 1.38; 95% CI: 1.11–1.72; p for trend = 0.0041). In conclusion, increased consumption of SSBs was closely linked with a higher prevalence of obesity and MetS in the Korean population.
Chitosan-Based Polyelectrolyte Complexes for Doxorubicin and Zoledronic Acid Combined Therapy to Overcome Multidrug Resistance
Authors: Giarra Simona , Zappavigna Silvia , Campani Virginia , Abate Marianna , Cossu Alessia Maria , Leonetti Carlo , Porru Manuela , Mayol Laura , Caraglia Michele , De Rosa Giuseppe
Published: October 2018

This study aimed to develop nanovectors co-encapsulating doxorubicin (Doxo) and zoledronic acid (Zol) for a combined therapy against Doxo-resistant tumors. Chitosan (CHI)-based polyelectrolyte complexes (PECs) prepared by ionotropic gelation technique were proposed. The influence of some experimental parameters was evaluated in order to optimize the PECs in terms of size and polydispersity index (PI). PEC stability was studied by monitoring size and zeta potential over time. In vitro studies were carried out on wild-type and Doxo-resistant cell lines, to assess both the synergism between Doxo and Zol, as well as the restoring of Doxo sensitivity. Polymer concentration, incubation time, and use of a surfactant were found to be crucial to achieving small size and monodisperse PECs. Doxo and Zol, only when encapsulated in PECs, showed a synergistic antiproliferative effect in all the tested cell lines. Importantly, the incubation of Doxo-resistant cell lines with Doxo/Zol co-encapsulating PECs resulted in the restoration of Doxo sensitivity.
Targeted Theranostic Nanoparticles for Brain Tumor Treatment
Authors: Mendes Maria , Sousa João José , Pais Alberto , Vitorino Carla
Published: October 2018

The poor prognosis and rapid recurrence of glioblastoma (GB) are associated to its fast-growing process and invasive nature, which make difficult the complete removal of the cancer infiltrated tissues. Additionally, GB heterogeneity within and between patients demands a patient-focused method of treatment. Thus, the implementation of nanotechnology is an attractive approach considering all anatomic issues of GB, since it will potentially improve brain drug distribution, due to the interaction between the blood–brain barrier and nanoparticles (NPs). In recent years, theranostic techniques have also been proposed and regarded as promising. NPs are advantageous for this application, due to their respective size, easy surface modification and versatility to integrate multiple functional components in one system. The design of nanoparticles focused on therapeutic and diagnostic applications has increased exponentially for the treatment of cancer. This dual approach helps to understand the location of the tumor tissue, the biodistribution of nanoparticles, the progress and efficacy of the treatment, and is highly useful for personalized medicine-based therapeutic interventions. To improve theranostic approaches, different active strategies can be used to modulate the surface of the nanotheranostic particle, including surface markers, proteins, drugs or genes, and take advantage of the characteristics of the microenvironment using stimuli responsive triggers. This review focuses on the different strategies to improve the GB treatment, describing some cell surface markers and their ligands, and reports some strategies, and their efficacy, used in the current research.
Synthesis of High Molecular Weight Polyester Using in Situ Drying Method and Assessment of Water Vapor and Oxygen Barrier Properties
Authors: Cheng Shouyun , Khan Burhan , Khan Fahad , Rabnawaz Muhammad
Published: October 2018

The preparation of renewable polyesters with good barrier properties is highly desirable for the packaging industry. Herein we report the synthesis of high molecular weight polyesters via an innovative use of an in situ drying agent approach and the barrier properties of the films formed from these polyesters. High number average molecular weight (Mn) semiaromatic polyesters (PEs) were synthesized via alternating ring-opening copolymerization (ROCOP) of phthalic anhydride (PA) and cyclohexene oxide (CHO) using a salen chromium(III) complex in the presence of 4-(dimethylamino)pyridine (DMAP) cocatalyst. The use of a calcium hydride (drying agent) was found to enhance the number Mn of the synthesized PEs, which reached up to 31.2 ku. To test the barrier properties, PE films were prepared by solvent casting approach and their barrier properties were tested in comparison poly(lactic acid) films. The PE films showed significantly improved water vapor and oxygen barrier properties compared to the commercial poly(lactic acid) (PLA) film that suggests the potential use of these PEs in in the food packaging industry.
Effect of Calcium Chloride as a Coagulant on the Properties of ESBR/Silica Wet Masterbatch Compound
Authors: Kim Woong , Ahn Byungkyu , Mun Hyunsung , Yu Eunho , Hwang Kiwon , Kim Donghyuk , Ryu Gyeongchan , Kim Wonho
Published: October 2018

When designing rubber compounds for high-performance tires, increasing the silica content can improve the wet traction performance but decreases the fuel efficiency. This trade-off relation makes it difficult to improve the two factors simultaneously. One approach is the development of silica wet masterbatch (WMB) technology for producing compounds containing a high silica content with good dispersion. The technology involves a step to mix surface-modified silica and rubber latex. The technique requires a coagulant to break up the micelles of the rubber latex and cause the surface-modified silica and the rubber molecules to co-coagulate due to van der Waals forces. In this study, the effect of coagulant type on the characteristics of silica surface, and the mechanical properties of the emulsion styrene-butadiene rubber (ESBR)/silica WMB compounds was investigated, as well as the abrasion properties and the viscoelastic properties of the vulcanizates.
The Ternary Heterostructures of BiOBr/Ultrathin g-C3N4/Black Phosphorous Quantum Dot Composites for Photodegradation of Tetracycline
Authors: Jiang Tianhao , Shang Chaoqun , Meng Qingguo , Jin Mingliang , Liao Hua , Li Ming , Chen Zhihong , Yuan Mingzhe , Wang Xin , Zhou Guofu
Published: October 2018

Herein, we synthesized BiOBr/ultrathin g-C3N4/ternary heterostructures modified with black phosphorous quantum dots using a simple water bath heating and sonication method. The ternary heterostructure was then used for the photocatalytic degradation of tetracycline in visible light, with an efficiency as high as 92% after 3 h of irradiation. Thus, the photodegradation efficiency is greatly improved compared to that of ultrathin g-C3N4, BiOBr, and black phosphorous quantum dots alone. The synthesized ternary heterostructure improves the charge separation efficiency, thus increasing the photodegradation efficiency. This work provides a new and efficient method for the degradation of antibiotics in the environment.
Medieval Archaeology Under the Canopy with LiDAR. The (Re)Discovery of a Medieval Fortified Settlement in Southern Italy
Authors: Masini Nicola , Gizzi Fabrizio Terenzio , Biscione Marilisa , Fundone Vincenzo , Sedile Michele , Sileo Maria , Pecci Antonio , Lacovara Biagio , Lasaponara Rosa
Published: October 2018

Despite the recognized effectiveness of LiDAR in penetrating forest canopies, its capability for archaeological prospection can be strongly limited in areas covered by dense vegetation for the detection of subtle remains scattered over morphologically complex areas. In these cases, an important contribution to improve the identification of topographic variations of archaeological interest is provided by LiDAR-derived models (LDMs) based on relief visualization techniques. In this paper, diverse LDMs were applied to the medieval site of Torre Cisterna to the north of Melfi (Southern Italy), selected for this study because it is located on a hilly area with complex topography and thick vegetation cover. These conditions are common in several places of the Apennines in Southern Italy and prevented investigations during the 20th century. Diverse LDMs were used to obtain maximum information and to compare the performance of both subjective (through visual inspections) and objective (through their automatic classification) methods. To improve the discrimination/extraction capability of archaeological micro-relief, noise filtering was applied to Digital Terrain Model (DTM) before obtaining the LDMs. The automatic procedure allowed us to extract the most significant and typical features of a fortified settlement, such as the city walls and a tower castle. Other small, subtle features attributable to possible buried buildings of a habitation area have been identified by visual inspection of LDMs. Field surveys and in-situ inspections were carried out to verify the archaeological points of interest, microtopographical features, and landforms observed from the DTM-derived models, most of them automatically extracted. As a whole, the investigations allowed (i) the rediscovery of a fortified settlement from the 11th century and (ii) the detection of an unknown urban area abandoned in the Middle Ages.
Probabilistic Damage Detection of a Steel Truss Bridge Model by Optimally Designed Bayesian Neural Network
Authors: Yin Tao , Zhu Hong-ping
Published: October 2018

Excellent pattern matching capability makes artificial neural networks (ANNs) a very promising approach for vibration-based structural health monitoring (SHM). The proper design of the network architecture with the suitable complexity is vital to the ANN-based structural damage detection. In addition to the number of hidden neurons, the type of transfer function used in the hidden layer cannot be neglected for the ANN design. Neural network learning can be further presented in the framework of Bayesian statistics, but the issues of selection for the hidden layer transfer function with respect to the Bayesian neural network has not yet been reported in the literature. In addition, most of the research works in the literature for addressing the predictive distribution of neural network output is only for a single target variable, while multiple target variables are rarely involved. In the present paper, for the purpose of probabilistic structural damage detection, Bayesian neural networks with multiple target variables are optimally designed, and the selection of the number of neurons, and the transfer function in the hidden layer, are carried out simultaneously to achieve a neural network architecture with suitable complexity. Furthermore, the nonlinear network function can be approximately linear by assuming the posterior distribution of network parameters is a sufficiently narrow Gaussian, and then the input-dependent covariance matrix of the predictive distribution of network output can be obtained with the Gaussian assumption for the situation of multiple target variables. Structural damage detection is conducted for a steel truss bridge model to verify the proposed method through a set of numerical case studies.
Digital Images Authentication Technique Based on DWT, DCT and Local Binary Patterns
Authors: Armas Vega Esteban Alejandro , Sandoval Orozco Ana Lucila , García Villalba Luis Javier , Hernandez-Castro Julio
Published: October 2018

In the last few years, the world has witnessed a ground-breaking growth in the use of digital images and their applications in the modern society. In addition, image editing applications have downplayed the modification of digital photos and this compromises the authenticity and veracity of a digital image. These applications allow for tampering the content of the image without leaving visible traces. In addition to this, the easiness of distributing information through the Internet has caused society to accept everything it sees as true without questioning its integrity. This paper proposes a digital image authentication technique that combines the analysis of local texture patterns with the discrete wavelet transform and the discrete cosine transform to extract features from each of the blocks of an image. Subsequently, it uses a vector support machine to create a model that allows verification of the authenticity of the image. Experiments were performed with falsified images from public databases widely used in the literature that demonstrate the efficiency of the proposed method.
Safety Culture Oversight: An Intangible Concept for Tangible Issues within Nuclear Installations
Authors: Bernard Benoît
Published: October 2018

Traced back to the Chernobyl Accident analysis (INSAG-1), the concept of safety culture is regarded as a central phenomenon influencing behaviors and values within high-risk organisations. Many studies have already been conducted on safety culture within nuclear installations. Describing a model designed to capture and assess safety culture observations, this paper intends to highlight the role of safety culture within the overall regulatory nuclear safety oversight, and to show how intangible cultural elements can lead to the identification of tangible safety issues.
The Hydrologic Role of Urban Green Space in Mitigating Flooding (Luohe, China)
Authors: Bai Tian , Mayer Audrey L. , Shuster William D. , Tian Guohang
Published: October 2018

Even if urban catchments are adequately drained by sewer infrastructures, flooding hotspots develop where ongoing development and poor coordination among utilities conspire with land use and land cover, drainage, and rainfall. We combined spatially explicit land use/land cover data from Luohe City (central China) with soil hydrology (as measured, green space hydraulic conductivity), topography, and observed chronic flooding to analyze the relationships between spatial patterns in pervious surface and flooding. When compared to spatial–structural metrics of land use/cover where flooding was commonly observed, we found that some areas expected to remain dry (given soil and elevation characteristics) still experienced localized flooding, indicating hotspots with overwhelmed sewer infrastructure and a lack of pervious surfaces to effectively infiltrate and drain rainfall. Next, we used curve numbers to represent the composite hydrology of different land use/covers within both chronic flooding and dry (non-flooding) circles of 750 m diameter, and local design storms to determine the anticipated average proportion of runoff. We found that dry circles were more permeable (curve number (mean ± std. error) = 74 ± 2, n = 25) than wetter, flooded circles (curve number = 87 ± 1). Given design storm forcing (20, 50, 100 years’ recurrence interval, and maximum anticipated storm depths), dry points would produce runoff of 26 to 35 percent rainfall, and wet points of 52 to 61 percent of applied rainfall. However, we estimate by simulation that runoff reduction benefits would decline once infiltration-excess (Hortonian) runoff mechanisms activate for storms with precipitation rates in excess of an average of 21 mm/h, contingent on antecedent moisture conditions. Our spatial metrics indicate that larger amounts and patches of dispersed green space mitigate flooding risk, while aggregating buildings (roofs) and green space into larger, separate areas exacerbates risk.
Changes in the Distribution of Temperature in a Coal Deposit and the Composition of Gases Emitted during Its Heating and Cooling
Authors: Więckowski Marek , Howaniec Natalia , Postnikov Eugene B. , Chorążewski Mirosław , Smoliński Adam
Published: October 2018

This article presents the results of tests conducted on a measuring system for monitoring changes in the distribution of temperature in a coal deposit during the heating and cooling phases, and their correlation with the analysis of the concentration of gases. The tests were conducted on five samples of hard coal collected in deposits mined in Poland. Measurements of the changes in temperature and changes in gas concentration were conducted from the temperature of 35 to 300 °C, for the heating phase, and from 300 to 35 °C, for the cooling phase. The percentage share of coal of given temperatures was calculated. When comparing the percentage share for the same temperature in the hot spot, for the heating and cooling phase, significant differences in the distribution of the given percentages were observed. Changes in gas concentrations during heating and cooling were analyzed and the dynamics of changes in gas concentrations were determined for the coals tested. Changes in the values of fire hazard indices were analyzed. There were significant differences in the concentration of gases and the values of fire hazard indices between the heating and the cooling phase. The application of different criteria to assess coal during heating and cooling was proposed.