bluuebunny/crossref_metadata_2025 huggingface dataset:
Kynurenic Acid Protects Against Ischemia/Reperfusion-Induced Retinal Ganglion Cell Death in Mice
Authors: Nahomi Rooban B. , Nam Mi-Hyun , Rankenberg Johanna , Rakete Stefan , Houck Julie A. , Johnson Ginger C. , Stankowska Dorota L. , Pantcheva Mina B. , MacLean Paul S. , Nagaraj Ram H.
Published: March 2020

Background: Glaucoma is an optic neuropathy and involves the progressive degeneration of retinal ganglion cells (RGCs), which leads to blindness in patients. We investigated the role of the neuroprotective kynurenic acid (KYNA) in RGC death against retinal ischemia/reperfusion (I/R) injury. Methods: We injected KYNA intravenously or intravitreally to mice. We generated a knockout mouse strain of kynurenine 3-monooxygenase (KMO), an enzyme in the kynurenine pathway that produces neurotoxic 3-hydroxykynurenine. To test the effect of mild hyperglycemia on RGC protection, we used streptozotocin (STZ) induced diabetic mice. Retinal I/R injury was induced by increasing intraocular pressure for 60 min followed by reperfusion and RGC numbers were counted in the retinal flat mounts. Results: Intravenous or intravitreal administration of KYNA protected RGCs against I/R injury. The I/R injury caused a greater loss of RGCs in wild type than in KMO knockout mice. KMO knockout mice had mildly higher levels of fasting blood glucose than wild type mice. Diabetic mice showed significantly lower loss of RGCs when compared with non-diabetic mice subjected to I/R injury. Conclusion: Together, our study suggests that the absence of KMO protects RGCs against I/R injury, through mechanisms that likely involve higher levels of KYNA and glucose.
Opposing Roles of FoxA1 and FoxA3 in Intrahepatic Cholangiocarcinoma Progression
Authors: Thanan Raynoo , Kaewlert Waleeporn , Sakonsinsiri Chadamas , Chaiprasert Timpika , Armartmuntree Napat , Muengsaen Duangkamon , Techasen Anchalee , Klanrit Poramate , Lert-itthiporn Worachart , Pinlaor Somchai , Pairojkul Chawalit
Published: March 2020

Cholangiocarcinoma (CCA), a malignancy of biliary epithelium, is related to liver stem cell deregulation. FoxAs are a group of transcription factors that play critical roles in liver stem cell differentiation. In this study, the expression levels of FoxAs (i.e., FoxA1, FoxA2 and FoxA3) were detected in intrahepatic CCA tissues and the functions of FoxAs were studied in CCA cell lines. FoxA1 and FoxA2 were mainly localized in the nuclei of normal bile duct (NBD) cells and some of the cancer cells. Low expression of FoxA1 in CCA tissues (72%) was significantly correlated with poor prognosis. FoxA3 expression of CCA cells was localized in the nucleus and cytoplasm, whereas it was slightly detected in NBDs. High expression of FoxA3 in cancer tissues (61%) was significantly related to high metastasis status. These findings suggest the opposing roles of FoxA1 and FoxA3 in CCA. Moreover, the FoxA1-over-expressing CCA cell line exhibited a significant reduction in proliferative and invasive activities compared to control cells. Knockdown of FoxA3 in CCA cells resulted in a significant decrease in proliferative and invasive activities compared with control cells. Taken together, in CCA, FoxA1 is down-regulated and has tumor suppressive roles, whereas FoxA3 is up-regulated and has oncogenic roles.
Genome-Wide Detection of Key Genes and Epigenetic Markers for Chicken Fatty Liver
Authors: Tan Xiaodong , Liu Ranran , Xing Siyuan , Zhang Yonghong , Li Qinghe , Zheng Maiqing , Zhao Guiping , Wen Jie
Published: March 2020

Chickens are one of the most important sources of meat worldwide, and the occurrence of fatty liver syndrome (FLS) is closely related to production efficiency. However, the potential mechanism of FLS remains poorly understood. An integrated analysis of data from whole-genome bisulfite sequencing and long noncoding RNA (lncRNA) sequencing was conducted. A total of 1177 differentially expressed genes (DEGs) and 1442 differentially methylated genes (DMGs) were found. There were 72% of 83 lipid- and glucose-related genes upregulated; 81% of 150 immune-related genes were downregulated in fatty livers. Part of those genes was within differentially methylated regions (DMRs). Besides, sixty-seven lncRNAs were identified differentially expressed and divided into 13 clusters based on their expression pattern. Some lipid- and glucose-related lncRNAs (e.g., LNC_006756, LNC_012355, and LNC_005024) and immune-related lncRNAs (e.g., LNC_010111, LNC_010862, and LNC_001272) were found through a co-expression network and functional annotation. From the expression and epigenetic profiles, 23 target genes (e.g., HAO1, ABCD3, and BLMH) were found to be hub genes that were regulated by both methylation and lncRNAs. We have provided comprehensive epigenetic and transcriptomic profiles on FLS in chicken, and the identification of key genes and epigenetic markers will expand our understanding of the molecular mechanism of chicken FLS.
Secretome of Senescent Adipose-Derived Mesenchymal Stem Cells Negatively Regulates Angiogenesis
Authors: Ratushnyy Andrey , Ezdakova Mariia , Buravkova Ludmila
Published: March 2020

Nowadays, paracrine regulation is considered as a major tool of mesenchymal stem cell (MSC) involvement in tissue repair and renewal in adults. Aging results in alteration of tissue homeostasis including neovascularization. In this study, we examined the influence of replicative senescence on the angiogenic potential of adipose-derived MSCs (ASCs). Angiogenic activity of conditioned medium (CM) from senescent and “young” ASCs was evaluated in chorioallantoic membrane (CAM) assay in ovo using Japanese quail embryos. Also, the formation of capillary-like tubes by human umbilical vein endothelial cells (HUVECs) in 3D basement membrane matrix “Matrigel” and HUVEC migration capacity were analyzed. Multiplex, dot-blot and gene expression analysis were performed to characterize transcription and production of about 100 angiogenesis-associated proteins. The results point to decreased angiogenic potential of senescent ASC secretome in ovo. A number of angiogenesis-associated proteins demonstrated elevation in CM after long-term cultivation. Meanwhile, VEGF (key positive regulator of angiogenesis) did not change transcription level and concentration in CM. Increasing both pro- (FGF-2, uPA, IL-6, IL-8 etc.) and antiangiogenic (IL-4, IP-10, PF4, Activin A, DPPIV etc.) factors was observed. Some proangiogenic genes were downregulated (IGF1, MMP1, TGFB3, PDGFRB, PGF). Senescence-associated secretory phenotype (SASP) modifications after long-term cultivation lead to attenuation of angiogenic potential of ASC.
Polymer Stabilized Cholesteric Liquid Crystal Siloxane for Temperature-Responsive Photonic Coatings
Authors: Zhang Weixin , Lub Johan , Schenning Albertus P.H.J. , Zhou Guofu , de Haan Laurens T.
Published: March 2020

Temperature-responsive photonic coatings are appealing for a variety of applications, including smart windows. However, the fabrication of such reflective polymer coatings remains a challenge. In this work, we report the development of a temperature-responsive, infrared-reflective coating consisting of a polymer-stabilized cholesteric liquid crystal siloxane, applied by a simple bar coating method. First, a side-chain liquid crystal oligosiloxane containing acrylate, chiral and mesogenic moieties was successfully synthesized via multiple steps, including preparing precursors, hydrosilylation, deprotection, and esterification reactions. Products of all the steps were fully characterized revealing a chain extension during the deprotection step. Subsequently, the photonic coating was fabricated by bar-coating the cholesteric liquid crystal oligomer on glass, using a mediator liquid crystalline molecule. After the UV-curing and removal of the mediator, a transparent IR reflective polymer-stabilized cholesteric liquid crystal coating was obtained. Notably, this fully cured, partially crosslinked transparent polymer coating retained temperature responsiveness due to the presence of non-reactive liquid-crystal oligosiloxanes. Upon increasing the temperature from room temperature, the polymer-stabilized cholesteric liquid crystal coating showed a continuous blue-shift of the reflection band from 1400 nm to 800 nm, and the shift was fully reversible.
Abrogation of IFN-γ Signaling May not Worsen Sensitivity to PD-1/PD-L1 Blockade
Authors: Vackova Julie , Piatakova Adrianna , Polakova Ingrid , Smahel Michal
Published: March 2020

Programmed cell death protein 1 (PD-1)/PD-1 ligand 1 (PD-L1) blockade is a promising therapy for various cancer types, but most patients are still resistant. Therefore, a larger number of predictive biomarkers is necessary. In this study, we assessed whether a loss-of-function mutation of the interferon (IFN)-γ receptor 1 (IFNGR1) in tumor cells can interfere with anti-PD-L1 therapy. For this purpose, we used the mouse oncogenic TC-1 cell line expressing PD-L1 and major histocompatibility complex class I (MHC-I) molecules and its TC-1/A9 clone with reversibly downregulated PD-L1 and MHC-I expression. Using the CRISPR/Cas9 system, we generated cells with deactivated IFNGR1 (TC-1/dIfngr1 and TC-1/A9/dIfngr1). In tumors, IFNGR1 deactivation did not lead to PD-L1 or MHC-I reduction on tumor cells. From potential inducers, mainly IFN-α and IFN-β enhanced PD-L1 and MHC-I expression on TC-1/dIfngr1 and TC-1/A9/dIfngr1 cells in vitro. Neutralization of the IFN-α/IFN-β receptor confirmed the effect of these cytokines in vivo. Combined immunotherapy with PD-L1 blockade and DNA vaccination showed that IFNGR1 deactivation did not reduce tumor sensitivity to anti-PD-L1. Thus, the impairment of IFN-γ signaling may not be sufficient for PD-L1 and MHC-I reduction on tumor cells and resistance to PD-L1 blockade, and thus should not be used as a single predictive marker for anti-PD-1/PD-L1 cancer therapy.
Root Development and Stress Tolerance in rice: The Key to Improving Stress Tolerance without Yield Penalties
Authors: Seo Deok Hyun , Seomun Subhin , Choi Yang Do , Jang Geupil
Published: March 2020

Roots anchor plants and take up water and nutrients from the soil; therefore, root development strongly affects plant growth and productivity. Moreover, increasing evidence indicates that root development is deeply involved in plant tolerance to abiotic stresses such as drought and salinity. These findings suggest that modulating root growth and development provides a potentially useful approach to improve plant abiotic stress tolerance. Such targeted approaches may avoid the yield penalties that result from growth–defense trade-offs produced by global induction of defenses against abiotic stresses. This review summarizes the developmental mechanisms underlying root development and discusses recent studies about modulation of root growth and stress tolerance in rice.
Oligogenic Origin of Differences of Sex Development in Humans
Authors: Camats Núria , Flück Christa E , Audí Laura
Published: March 2020

Sex development is a very complex biological event that requires the concerted collaboration of a large network of genes in a spatial and temporal correct fashion. In the past, much has been learned about human sex development from monogenic disorders/differences of sex development (DSD), but the broad spectrum of phenotypes in numerous DSD individuals remains a conundrum. Currently, the genetic cause of less than 50% of DSD individuals has been solved and oligogenic disease has been proposed. In recent years, multiple genetic hits have been found in individuals with DSD thanks to high throughput sequencing. Our group has been searching for additional genetic hits explaining the phenotypic variability over the past years in two cohorts of patients: 46,XY DSD patients carriers of NR5A1 variants and 46,XY DSD and 46,XX DSD with MAMLD1 variants. In both cohorts, our results suggest that the broad phenotypes may be explained by oligogenic origin, in which multiple hits may contribute to a DSD phenotype, unique to each individual. A search for an underlying network of the identified genes also revealed that a considerable number of these genes showed interactions, suggesting that genetic variations in these genes may affect sex development in concert.
Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance
Authors: Depciuch Joanna , Klębowski Bartosz , Stec Małgorzata , Szatanek Rafał , Węglarczyk Kazimierz , Baj-Krzyworzeka Monika , Parlińska-Wojtan Magdalena , Baran Jarek
Published: March 2020

Colon cancer constitutes 33% of all cancer cases in humans and the majority of patients with metastatic colon cancer still have poor prognosis. An important role in cancer development is the communication between cancer and normal cells. This may occur, among others, through extracellular vesicles (including microvesicles) (MVs), which are being released by both types of cells. MVs may regulate a diverse range of biological processes and are considered as useful cancer biomarkers. Herein, we show that similarity in the general chemical composition between colon cancer cells and their corresponding tumor-derived microvesicles (TMVs) does exist. These results have been confirmed by spectroscopic methods for four colon cancer cell lines: HCT116, LoVo, SW480, and SW620 differing in their aggressiveness/metastatic potential. Our results show that Raman and Fourier Transform InfraRed (FTIR) analysis of the cell lines and their corresponding TMVs did not differ significantly in the characterization of their chemical composition. However, hierarchical cluster analysis of the data obtained by both of the methods revealed that only Raman spectroscopy provides results that are in line with the molecular classification of colon cancer, thus having potential clinical relevance.
Newborn Screening for CF across the Globe—Where Is It Worthwhile?
Authors: Scotet Virginie , Gutierrez Hector , Farrell Philip M.
Published: March 2020

Newborn screening (NBS) for cystic fibrosis (CF) has been performed in many countries for as long as four decades and has transformed the routine method for diagnosing this genetic disease and improved the quality and quantity of life for people with this potentially fatal disorder. Each region has typically undertaken CF NBS after analysis of the advantages, costs, and challenges, particularly regarding the relationship of benefits to risks. The very fact that all regions that began screening for CF have continued their programs implies that public health and clinical leaders consider early diagnosis through screening to be worthwhile. Currently, many regions where CF NBS has not yet been introduced are considering options and in some situations negotiating with healthcare authorities as policy and economic factors are being debated. To consider the assigned question (where is it worthwhile?), we have completed a worldwide analysis of data and factors that should be considered when CF NBS is being contemplated. This article describes the lessons learned from the journey toward universal screening wherever CF is prevalent and an analytical framework for application in those undecided regions. In fact, the lessons learned provide insights about what is necessary to make CF NBS worthwhile.
Roadworks Warning—Closure of a Lane, the Impact of C-ITS Messages
Authors: Agriesti Serio Angelo Maria , Studer Luca , Marchionni Giovanna , Gandini Paolo , Qu Xiaobo
Published: March 2020

By now, it is widely acknowledged among stakeholders and academia that infrastructures will have to be composed both by a physical component and a digital one. The deployment of technologies exploiting dedicated short-range communications is viewed as the most cost-effective solution to face the foreseen growth of mobility. Still, little has been done to define the best implementation logic of DSRC. Aim of this paper is to frame the possible impacts arising by the implementation of a cooperative intelligent transport system (C-ITS)-use case: roadworks warning—closure of a lane, and, in order to achieve this result, microsimulations are exploited. The results are intended to support both road operators and car-makers in defining the best operational logics and the possible benefits achievable by presenting the cooperative message at a certain distance for certain market penetrations. Moreover, if the C-ITS message actually entails benefits or simply disrupts the upstream traffic should be assessed in advance, before implementing the system. The obtained results show that the risk of disruption and of reduction in traffic efficiency arises at lower market penetration levels. Nevertheless, a consistent trend in delay reduction is recorded upstream the roadworks, the highest reduction being equal to 8.66%. Moreover, the average speed at the roadworks entrance on the closing lane increases by a difference equal to around 10 km/h, while the average time in the queue at the highest market penetration reduces by 60 s on the open lane and 25 s on the closing one. These presented results reflect the way the traffic shifts from the slow to the fast lane thanks to the C-ITS system and effectively frames both the potentialities and the risks of the system.
Metal–Dithiolene Bonding Contributions to Pyranopterin Molybdenum Enzyme Reactivity
Authors: Yang Jing , Enemark John H. , Kirk Martin L.
Published: March 2020

Here we highlight past work on metal–dithiolene interactions and how the unique electronic structure of the metal–dithiolene unit contributes to both the oxidative and reductive half reactions in pyranopterin molybdenum and tungsten enzymes. The metallodithiolene electronic structures detailed here were interrogated using multiple ground and excited state spectroscopic probes on the enzymes and their small molecule analogs. The spectroscopic results have been interpreted in the context of bonding and spectroscopic calculations, and the pseudo-Jahn–Teller effect. The dithiolene is a unique ligand with respect to its redox active nature, electronic synergy with the pyranopterin component of the molybdenum cofactor, and the ability to undergo chelate ring distortions that control covalency, reduction potential, and reactivity in pyranopterin molybdenum and tungsten enzymes.
Rural and Urban Population Differences in the Association of Dietary Calcium with Blood Pressure and Hypertension Occurrence: Analysis of Longitudinal Data from the China Health and Nutrition Survey
Authors: Ngo Nkondjock Victorine-Raïssa , Li Ying , Thérèse Martin Cheteu , Reem Alariqi
Published: March 2020

The relationship of dietary calcium intake (DCI) to blood pressure (BP) and hypertension occurrence (HTN) in the general population is controversial. Few studies have reported the impact of DCI on high blood pressure (HBP) and HTN in rural and urban populations (RPs and UPs, respectively). In this study, we assessed how DCI is related to BP and HTN among the RP and the UP. This retrospective study used data from the Chinese Health and Nutrition Survey (CHNS) for 2000–2009. We analyzed 12,052 records from 3013 participants using path analysis and logistic regressions. DCI and HTN were significantly higher in the UP compared to the RP. When UPs and RPs were analyzed together, a 1 unit increase in DCI decreased systolic blood pressure (SBP) by 0.01 (p = 0.002) but had no effect on diastolic blood pressure. Males vs. females from the RP presented significant risk of HTN by the high DCI (OR = 2.3 (1.6–3.6)). DCI and its association with BP varied based on living environment and sex.
Do Nutritional Factors Interact with Chronic Musculoskeletal Pain? A Systematic Review
Authors: Elma Ömer , Yilmaz Sevilay Tümkaya , Deliens Tom , Coppieters Iris , Clarys Peter , Nijs Jo , Malfliet Anneleen
Published: March 2020

Dietary patterns may play an important role in musculoskeletal well-being. However, the link between dietary patterns, the components of patients’ diet, and chronic musculoskeletal pain remains unclear. Therefore, the purpose of this review was to systematically review the literature on the link between dietary patterns, the components of patients’ diet and chronic musculoskeletal pain. This review was conducted following the “Preferred Reporting Items for Systematic reviews and Meta-Analyses” (PRISMA) guidelines and was registered in PROSPERO with the registration number CRD42018110782. PubMed, Web of Science, and Embase online databases were searched. After screening titles and abstracts of 20,316 articles and full texts of 347 articles, 12 eligible articles were included in this review, consisting of nine experimental and three observational studies. Seven out of nine experimental studies reported a pain-relieving effect of dietary changes. Additionally, protein, fat, and sugar intake were found to be associated with pain intensity and pain threshold. In conclusion, plant-based diets might have pain relieving effects on chronic musculoskeletal pain. Patients with chronic rheumatoid arthritis pain can show inadequate intake of calcium, folate, zinc, magnesium, and vitamin B6, whilst patients with fibromyalgia can show a lower intake of carbohydrates, proteins, lipids, vitamin A-E-K, folate, selenium, and zinc. Chronic pain severity also shows a positive relation with fat and sugar intake in osteoarthritis, and pain threshold shows a positive association with protein intake in fibromyalgia.
KCP10043F Represses the Proliferation of Human Non-Small Cell Lung Cancer Cells by Caspase-Mediated Apoptosis via STAT3 Inactivation
Authors: Lee Jeong-Hun , Lee Hwi-Ho , Ryu Ki Deok , Kim Misong , Ko Dohyeong , Chung Kyung-Sook , Hassan Ahmed H.E. , Lee Seung Hyeun , Lee Jae Yeol , Lee Kyung-Tae
Published: March 2020

We previously reported that 4-(4-fluorobenzylcarbamoylmethyl)-3-(4-cyclohexylphenyl)-2-[3-(N,N-dimethylureido)-N′-methylpropylamino]-3,4-dihydroquinazoline (KCP10043F) can induce G1-phase arrest and synergistic cell death in combination with etoposide in lung cancer cells. Here, we investigated the underlying mechanism by which KCP10043F induces cell death in non-small cell lung cancer (NSCLC). Propidium iodide (PI) and annexin V staining revealed that KCP10043F-induced cytotoxicity was caused by apoptosis. KCP10043F induced a series of intracellular events: (1) downregulation of Bcl-2 and Bcl-xL and upregulation of Bax and cleaved Bid; (2) loss of mitochondrial membrane potential; (3) increase of cytochrome c release; (4) cleavage of procaspase-8, procaspase-9, procaspase-3, and poly (ADP-ribose) polymerase (PARP). In addition, KCP10043F exhibited potent inhibitory effects on constitutive or interleukin-6 (IL-6)-induced signal transducer and activator of transcription (STAT3) phosphorylation and STAT3-regulated genes including survivin, Mcl-1, and cyclin D1. Furthermore, STAT3 overexpression attenuated KCP10043F-induced apoptosis and the cleavage of caspase-9, caspase-3, and PARP. Docking analysis disclosed that KCP10043F could bind to a pocket in the SH2 domain of STAT3 and prevent STAT3 phosphorylation. The oral administration of KCP10043F decreased tumor growth in an A549 xenograft mouse model, as associated with the reduced phosphorylated STAT3, survivin, Mcl-1, and Bcl-2 expression and increased TUNEL staining and PARP cleavage in tumor tissues. Collectively, our data suggest that KCP10043F suppresses NSCLC cell growth through apoptosis induction via STAT3 inactivation.
Physical Activity: A Missing Link in Asthma Care
Authors: Panagiotou Marios , Koulouris Nikolaos G. , Rovina Nikoletta
Published: March 2020

Asthma is the commonest respiratory disease and one of unceasingly increasing prevalence and burden. As such, asthma has attracted a major share or scientific interest and clinical attention. With the various clinical and pathophysiological aspects of asthma having been extensively investigated, the important association between asthma and physical activity remains underappreciated and insufficiently explored. Asthma impacts adversely on physical activity. Likewise, poor physical activity may lead to worse asthma outcomes. This concise clinical review presents the current recommendations for physical activity, discusses the available evidence on physical activity in asthma, and examines the causes of low physical activity in adult asthmatic patients. It also reviews the effect of daily physical activity and exercise training on the pathology and clinical outcomes of asthma. Finally, it summarizes the evidence on interventions targeting physical activity in asthma.
Stereolithographic Surgical Guide with a Combination of Tooth and Bone Support: Accuracy of Guided Implant Surgery in Distal Extension Situation
Authors: Lin Chia-Cheng , Ishikawa Masahiro , Maida Takeo , Cheng Hsin-Chung , Ou Keng-Liang , Nezu Takashi , Endo Kazuhiko
Published: March 2020

A distal free-end situation could result in insufficient stability of the surgical guide, and could reduce accuracy of the static guided implant surgery (sGIS). The purpose of this study was to investigate the accuracy of sGIS using a combination tooth-and-bone supported stereolithographic (SLA) surgical guide in distal extension situation. Thirty dentists, each placed three implants at the Federal Dentaire Internationale (FDI) teeth positions #46, #47 (a distal extension situation), and #36 (a single tooth gap) via the surgical guide on a model fixed to a manikin. Pre- and post-operative computed tomography (CT) images of the models were superimposed, and the positional and angular deviations of the implants were measured with metrology software. An analysis of variance (ANOVA) test was performed to evaluate the intergroup differences. No significant differences were found for all the positional and angular deviations among the three implant sites, except the bucco-lingual deviation at the implant platform in the #47 position (0.43 ± 0.19 mm) that was significantly larger than the #46 (0.21 ± 0.14 mm) and #36 (0.24 ± 0.25 mm) positions (p < 0.0001). Within the limits of this study, we conclude that, in distal extension situation of missing mandibular molars, adding a bone-supported strut in the distal part of the surgical guide can be beneficial to the accuracy of the sGIS.
Analysis of Cervical Spine Alignment and its Relationship with Other Spinopelvic Parameters after Laminoplasty in Patients with Degenerative Cervical Myelopathy
Authors: Kim Seok Woo , Jang Seung Bo , Lee Hyung Min , Lee Jeong Hwan , Lee Min Uk , Kim Jeong Woo , Yee Jae Sung
Published: March 2020

For patients with kyphosis of the cervical spine, laminoplasty is usually incapable of improving neurological symptoms as it worsens kyphotic alignment. Thus, laminoplasty is not recommended in the presence of kyphotic alignment. Nevertheless, laminoplasty may be selected for myelopathy due to multiple-segment intervertebral disc herniation or ossification of posterior longitudinal ligament despite kyphotic alignment. This study examined whether cervical alignment influences surgical outcomes. Cervical alignment before the surgery was classified into lordosis and non-lordosis, and the non-lordosis group was subclassified into reducible and non-reducible groups to determine the change in cervical alignment before and after the surgery and to analyze its relationship with spinopelvic parameters. The lordosis group showed an increase in upper cervical motion (C0-2 Range of Motion (ROM), C0-2ROM/C0-7ROM) after surgery, while the non-lordosis group exhibited a decrease in C2-7ROM and C0-7ROM. The C0-2ROM was maintained without any reduction in the reducible group, while there was no significant change in cervical alignment and ROM of the non-reducible group. None of these changes showed significant association with the spinopelvic parameters of other sites. However, having a non-reducible type non-lordosis is not a proper indication for laminoplasty, as it does not change the alignment after surgery. Therefore, cervical alignment and reducibility should be identified before surgery.
Presence of Antibodies Binding to Negative Elongation Factor E in Sarcoidosis
Authors: Baerlecken Niklas , Pursche Nils , Witte Torsten , Kniesch Katja , Höpfner Marius , Ernst Diana , Moosig Frank , Seeliger Benjamin , Prasse Antje
Published: March 2020

Sarcoidosis is characterized by multiorgan involvement and granulomatous inflammation. Its origin is unknown and the potential role of autoimmunity has not been sufficiently determined. We investigated the presence of autoantibodies in sarcoidosis using protein array technology. The derivation cohort consisted of patients with sarcoidosis (n = 25) and controls including autoimmune disease and blood donors (n = 246). In addition, we tested a validation cohort including pulmonary sarcoidosis patients (n = 58) and healthy controls (n = 13). Initially, sera of three patients with sarcoidosis were screened using a protein array with 28.000 proteins against controls. Thereby we identified the Negative Elongation Factor E (NELF-E) as an autoantigen. With confirmatory Enzyme-linked Immunosorbent Assay (ELISA)testing, 29/82 patients (35%) with sarcoidosis had antibodies against NELF-E of the Immunoglobulin (Ig) G type, whereas 18/253 (7%) sera of the controls were positive for NELF-E. Clinically, there was an association of the frequency of NELF-E antibody detection with lung parenchymal involvement and corresponding x-ray types. NELF-E autoantibodies are associated with sarcoidosis and should be further investigated.
Evaluating the Efficacy of L-N-acetylcysteine and Dexamethasone in Combination to Provide Otoprotection for Electrode Insertion Trauma
Authors: Eshraghi Adrien A. , Shahal David , Davies Camron , Mittal Jeenu , Shah Viraj , Bulut Erdogan , Garnham Carolyn , Sinha Priyanka , Mishra Dibyanshi , Marwede Hannah , Mittal Rahul
Published: March 2020

Background: Electrode insertion trauma (EIT) during cochlear implantation (CI) can cause loss of residual hearing. L-N-acetylcysteine (L-NAC) and dexamethasone (Dex) have been individually shown to provide otoprotection albeit at higher concentrations that may be associated with adverse effects. Objective/Aims: The aim of this study is to determine whether L-NAC and Dex could be combined to decrease their effective dosage. Materials and Methods: The organ of Corti (OC) explants were divided into various groups: 1) control; 2) EIT; 3) EIT treated with different concentrations of Dex; 4) EIT treated with different concentrations of L-NAC; 5) EIT treated with L-NAC and Dex in combination. Hair cell (HC) density, levels of oxidative stress, proinflammatory cytokines and nitric oxide (NO) was determined. Results: There was a significant loss of HCs in explants subjected to EIT compared to the control group. L-NAC and Dex in combination was able to provide significant otoprotection at lower concentrations compared to individual drugs. Conclusions and Significance: A combination containing L-NAC and Dex is effective in protecting sensory cells at lower protective doses than each compound separately. These compounds can be combined allowing a decrease of potential side effects of each compound and providing significant otoprotection for EIT.
Improving the Clinical Outcome in Stroke Patients Receiving Thrombolytic or Endovascular Treatment in Korea: from the SECRET Study
Authors: Kim Young Dae , Heo Ji Hoe , Yoo Joonsang , Park Hyungjong , Kim Byung Moon , Bang Oh Young , Kim Hyeon Chang , Han Euna , Kim Dong Joon , Heo JoonNyung , Kim Minyoung , Choi Jin Kyo , Lee Kyung-Yul , Lee Hye Sun , Shin Dong Hoon , Choi Hye-Yeon , Sohn Sung-Il , Hong Jeong-Ho , Baek Jang-Hyun , Kim Gyu Sik , Seo Woo-Keun , Chung Jong-Won , Kim Seo Hyun , Song Tae-Jin , Han Sang Won , Park Joong Hyun , Kim Jinkwon , Jung Yo Han , Cho Han-Jin , Ahn Seong Hwan , Lee Sung Ik , Seo Kwon-Duk , Nam Hyo Suk
Published: March 2020

We investigated whether there was an annual change in outcomes in patients who received the thrombolytic therapy or endovascular treatment (EVT) in Korea. This analysis was performed using data from a nationwide multicenter registry for exploring the selection criteria of patients who would benefit from reperfusion therapies in Korea. We compared the annual changes in the modified Rankin scale (mRS) at discharge and after 90 days and the achievement of successful recanalization from 2012 to 2017. We also investigated the determinants of favorable functional outcomes. Among 1230 included patients, the improvement of functional outcome at discharge after reperfusion therapy was noted as the calendar year increased (p < 0.001). The proportion of patients who were discharged to home significantly increased (from 45.6% in 2012 to 58.5% in 2017) (p < 0.001). The successful recanalization rate increased over time from 78.6% in 2012 to 85.1% in 2017 (p = 0.006). Time from door to initiation of reperfusion therapy decreased over the years (p < 0.05). These secular trends of improvements were also observed in 1203 patients with available mRS data at 90 days (p < 0.05). Functional outcome was associated with the calendar year, age, initial stroke severity, diabetes, preadmission disability, intervals from door to reperfusion therapy, and achievement of successful recanalization. This study demonstrated the secular trends of improvement in functional outcome and successful recanalization rate in patients who received reperfusion therapy in Korea.
Regulating Retinoic Acid Availability during Development and Regeneration: The Role of the CYP26 Enzymes
Authors: Roberts Catherine
Published: March 2020

This review focuses on the role of the Cytochrome p450 subfamily 26 (CYP26) retinoic acid (RA) degrading enzymes during development and regeneration. Cyp26 enzymes, along with retinoic acid synthesising enzymes, are absolutely required for RA homeostasis in these processes by regulating availability of RA for receptor binding and signalling. Cyp26 enzymes are necessary to generate RA gradients and to protect specific tissues from RA signalling. Disruption of RA homeostasis leads to a wide variety of embryonic defects affecting many tissues. Here, the function of CYP26 enzymes is discussed in the context of the RA signalling pathway, enzymatic structure and biochemistry, human genetic disease, and function in development and regeneration as elucidated from animal model studies.
Arthrocentesis and Sodium Hyaluronate Infiltration in Temporomandibular Disorders Treatment. Clinical and MRI Evaluation
Authors: Santagata Mario , De Luca Roberto , Lo Giudice Giorgio , Troiano Antonio , Lo Giudice Giuseppe , Corvo Giovanni , Tartaro Gianpaolo
Published: March 2020

Arthrocentesis in temporomandibular joint disorders can be associated with the intra-articular infiltration of various drugs with the objective of increase treatment efficacy. The aim of this study was to evaluate the clinical indexes variation in patients affected by temporomandibular joint disorders treated with arthrocentesis and sodium hyaluronate (SH) injections. A total of 28 patients suffering from temporomandibular joint disorders underwent one cycle of five arthrocentesis and infiltrations of sodium hyaluronate. Spontaneous mouth opening improved from 36.3 ± 7.5 mm to 45.1 ± 1.9 mm at six months follow-up. A significant reduction in the pain at rest and during mastication mean values emerged at follow-up (p < 0.0001). The mean masticatory efficiency, evaluated through a visual analogic scale, showed improvement at the follow-up period, highlighted by the increase of mean value from a baseline of 3.1 ± 1.2 to a mean value of 8.5 ± 1.2 (p < 0.0001). The mean severity of the joint damage at baseline time was 2.4 ± 0.9 and decreased to 0.4 ± 0.3 at the end of the follow-up period. The decrease in values is confirmed by statistical test (p < 0.05). Our data show how arthrocentesis integrated with sodium hyaluronate infiltrations performed under local anesthesia is a valid method of treating temporomandibular joint disorders.
A Survey of Deep Learning-Based Source Image Forensics
Authors: Yang Pengpeng , Baracchi Daniele , Ni Rongrong , Zhao Yao , Argenti Fabrizio , Piva Alessandro
Published: March 2020

Image source forensics is widely considered as one of the most effective ways to verify in a blind way digital image authenticity and integrity. In the last few years, many researchers have applied data-driven approaches to this task, inspired by the excellent performance obtained by those techniques on computer vision problems. In this survey, we present the most important data-driven algorithms that deal with the problem of image source forensics. To make order in this vast field, we have divided the area in five sub-topics: source camera identification, recaptured image forensic, computer graphics (CG) image forensic, GAN-generated image detection, and source social network identification. Moreover, we have included the works on anti-forensics and counter anti-forensics. For each of these tasks, we have highlighted advantages and limitations of the methods currently proposed in this promising and rich research field.
Automated Harmonic Signal Removal Technique Using Stochastic Subspace-Based Image Feature Extraction
Authors: Abu Hasan Muhammad Danial Bin , Ahmad Zair Asrar Bin , Leong Mohd Salman , Hee Lim Meng
Published: March 2020

This paper presents automated harmonic removal as a desirable solution to effectively identify and discard the harmonic influence over the output signal by neglecting any user-defined parameter at start-up and automatically reconstruct back to become a useful output signal prior to system identification. Stochastic subspace-based algorithms (SSI) methods are the most practical tool due to the consistency in modal parameters estimation. However, it will be problematic when applied to structures with rotating machines and the presence of harmonic excitations. Difficulties arise when automating this procedure without any human interaction and the problem is still unresolved because stochastic subspace-based algorithms (SSI) methods still require parameters (the maximum within-cluster distance) that are compulsory to be defined at start-up for each analysis of the dataset. Thus, the use of image-based feature extraction for clustering and classification of harmonic components and structural poles directly from a stabilization diagram and for modal system identification is the focus of the present paper. As a fundamental necessary condition, the algorithm has been assessed first from computed numerical responses and then applied to the experimental dataset with the presence of harmonic excitation. Results of the proposed approach for estimating modal parameters demonstrated very high accuracy and exhibited consistent results before and after removing harmonic components from the response signal.
Hydrostatic Bandsaw Blade Guides for Natural Stone-Cutting Applications
Authors: Ahsan Ammar , Kenney Kyle , Kröger Jonas , Böhm Stefan
Published: March 2020

In a bandsaw machine, the blade guides provide additional stiffness and help to align the blade near the cutting region. Typically, these are either in the form of blocks made of carbide or ceramics or as sealed bearings. Abrasive particles, generated while cutting hard and brittle materials like natural stones, settle between the contact surfaces of the guides and the blade causing wear and premature failure. The hydrostatic guide system, as presented in this work, is a contactless blade guiding method that uses the force of several pressurized water jets to align the blade to the direction of the cut. For this investigation, cutting tests were performed on a marble block using a galvanic diamond coated bandsaw blade with the upper roller guides replaced by hydrostatic guides. The results show that the hydrostatic guides help to reduce the passive force to a constant near zero in contrast with the traditional guides. This also resulted in reduced surface roughness of the stone plates that were cut, indicating a reduction in laterial vibration of the band. Additionally, it has also been shown that using hydrostatic guides the bandsaw blade can be tilted to counter the bandsaw drift, opening opportunities for further research in active alignment control. This original research work has shown that the hydrostatic guide systems are capable of replacing, and in fact, perform better than state-of-the-art bearing or block guides, particularly for stone-cutting applications.
Development of a Novel Tuning Approach of the Notch Filter of the Servo Feed Drive System
Authors: Liu Chung-Ching , Tsai Meng-Shiun , Hong Mao-Qi , Tang Pu-Yang
Published: March 2020

To alleviate the vibration effect of the feed drive system, a traditional approach is to apply notch filters to suppress vibrations in the velocity loop. The current approach is to adjust the notch filter parameters such as the center frequency, damping and depth based on observing the frequency response diagram of servo velocity closed loop. In addition, the notch filters are generally provided in the velocity loop control for the commercialized controllers such as FANUC, Siemens, etc. However, the resonance of the transmission system also appears in the position loop when the linear scale is used as the position feedback. The notch filter design without consideration of the resonance behavior of the position loop might cause degradation of performance. To overcome this problem, the paper proposes an innovative method which could automatically determine the optimal parameters of the notch filter under the consideration of resonance behavior of position loop. With the optimal parameters, it is found that both the gains of position and velocity loop controller could be increased such that the bandwidths of the position/velocity loops are higher. Based on the simulation results, the rising time is improved by 33% and the time for reaching the steady state is improved by 72% as comparing the cases of using the optimal approach and traditional approach.
Near-Trapping on a Four-Column Structure and the Reduction of Wave Drift Forces Using Optimized Method
Authors: He Guanghua , Zhang Zhigang , Wang Wei , Wang Zhengke , Jing Penglin
Published: March 2020

The near-trapping phenomenon, which can lead to high wave elevations and large wave drift forces, is investigated by a floating four-column structure. To solve this wave-structure interaction problem, a numerical model is established by combining the wave interaction theory with a higher-order boundary element method. Based on this numerical model, behaviors of scattered waves at near-trapping conditions are studied; and the superposition principle of free-surface waves is introduced to understand this near-trapping phenomenon. To avoid the near-trapping phenomenon and protect the structure, a way for rotating the structure to change the wave-approach angle is adopted, and improvements of the wave elevations around the structure and the wave drift forces acting on each column are found. Moreover, a genetic-algorithm-based optimization method is adopted in order to minimize the total wave drift force acting on the whole structure at various wavenumbers by controlling the draft of floating bodies, the wave-approach angle and the separation distance between adjacent floating bodies. With the final optimized parameters, the wave drift forces both on each column and on the whole structure can be significantly reduced. The optimized arrangement obtained from a certain wavenumber can work not only at this target wavenumber but also at a range of wavenumbers.
Effects of Repeated Sand Replenishment Projects on Runs of a Beach-Spawning Fish, the California Grunion
Authors: Martin Karen L. M. , Adams Loni C.
Published: March 2020

Beach habitats are diminishing globally, particularly in urban areas, as sea-level rise, erosion, and shoreline hardening, along with reduced sediment inputs, combine to squeeze the coast. In California, USA an endemic marine fish, the California grunion, spawns on sandy beaches during late-night spring tides. Its unique recreational fishery is managed by the California Department of Fish and Wildlife. The City of Oceanside, CA contracts for annual harbor dredging and, after testing, places the sandy sediment on its public beach. The effects on local beach wildlife from this annual sand replenishment are not known. We examined the effect of this repeated activity as a case study over three years on the spawning runs of the California grunion. Some spawning runs occurred in all three years, but the fish avoided areas with high scarps in the intertidal zone that developed following sand placement activity. Grunion spawning runs have declined in the habitat range as a whole over the past two decades, and those in Oceanside have declined to an even greater extent. Increasing sandy beach habitat can be beneficial to wildlife, but the method of placement, timing of the project, and fate of the beach afterward can modulate or prevent beneficial effects. Frequent repetition of sand placement may accumulate impacts without allowing sufficient time for the ecosystem to recover. Rather than improving the habitat, these repeated projects in Oceanside may degrade the spawning habitat for the grunion. Alternative discharge methods and locations, slope and elevation designs, sediment volumes, and greater care in beach fill practices should be implemented to reduce future impacts.
Analytical Gradients of Dynamic Conditional Correlation Models
Authors: Caporin Massimiliano , Lucchetti Riccardo (Jack) , Palomba Giulio
Published: March 2020

We provide the analytical gradient of the full model likelihood for the Dynamic Conditional Correlation (DCC) specification by Engle (2002), the generalised version by Cappiello et al. (2006), and of the cDCC model by Aielli(2013). We discuss how the gradient might be further extended by introducing elements related to the conditional variance parameters, and discuss the issue arising from the estimation of constrained and/or reparametrised versions of the model. A computational simulation compares analytical versus numerical gradients, with a view to parameter estimation; we find that analytical differentiation yields more efficiency and improved accuracy.
Post-NAFTA Changes in Peasant Land Use—The Case of the Pátzcuaro Lake Watershed Region in the Central-West México
Authors: Astier Marta , Orozco-Ramírez Quetzalcóatl , Walker Robert , Galván-Miyoshi Yankuic , González-Esquivel Carlos , Simmons Cynthia S.
Published: March 2020

Rural life in México has changed drastically over the past several decades in the wake of structural reforms in the 1980s and the North American Free Trade Agreement (NAFTA) implemented in 1994. Researchers predicted dire consequences for smallholder farmers following trade liberalization and in certain respects the prophecies have been fulfilled. Indeed, many regions experienced significant out-migration as smallholders, unable to compete with global maize imports without price subsidies, sold or abandoned their lands, making way for the expansion of industrial agriculture into forests, secondary vegetation and primary crops. Nevertheless, many smallholders have adapted to the new economic environment with farming systems that manage risk by diversifying portfolios to incorporate commercialized maize and livestock production. This article examines the evolution of smallholder farming systems since the mid 1980s, when the impact of neoliberal reforms emerged, using data collected from field research on 130 smallholder farms in the Pátzcuaro Lake Watershed (PLW) in the State of Michoacán. Farmers in the PLW have been characterized as traditional peasant farmers, planting crops for subsistence, including a diverse array of domestic maize varieties and practicing limited animal husbandry with chickens, turkeys, pigs, an oxen and a cow or two for milk. But the results presented in this article show that the traditional peasant farming systems in the region have changed substantially to a highly diversified agriculture-cattle-forest system. Most notable changes include the use of fertilizers and pesticides; and the increase in livestock herd and reorientation to beef production. The results demonstrate the resilience of smallholder farmers, while at the same time raising potential concern that increased reliance on livestock and beef production specialization, might lead to shifts in farming systems that replace domestic maize varieties with hybrid corn used primarily for animal feed and thereby leaving vulnerable the genetic reservoir of traditional maize landraces.