bluuebunny/crossref_metadata_2025 huggingface dataset:
Task-Level Authoring for Remote Robot Teleoperation
Authors: Senft Emmanuel , Hagenow Michael , Welsh Kevin , Radwin Robert , Zinn Michael , Gleicher Michael , Mutlu Bilge
Published: September 2021

Remote teleoperation of robots can broaden the reach of domain specialists across a wide range of industries such as home maintenance, health care, light manufacturing, and construction. However, current direct control methods are impractical, and existing tools for programming robot remotely have focused on users with significant robotic experience. Extending robot remote programming to end users, i.e., users who are experts in a domain but novices in robotics, requires tools that balance the rich features necessary for complex teleoperation tasks with ease of use. The primary challenge to usability is that novice users are unable to specify complete and robust task plans to allow a robot to perform duties autonomously, particularly in highly variable environments. Our solution is to allow operators to specify shorter sequences of high-level commands, which we call task-level authoring, to create periods of variable robot autonomy. This approach allows inexperienced users to create robot behaviors in uncertain environments by interleaving exploration, specification of behaviors, and execution as separate steps. End users are able to break down the specification of tasks and adapt to the current needs of the interaction and environments, combining the reactivity of direct control to asynchronous operation. In this paper, we describe a prototype system contextualized in light manufacturing and its empirical validation in a user study where 18 participants with some programming experience were able to perform a variety of complex telemanipulation tasks with little training. Our results show that our approach allowed users to create flexible periods of autonomy and solve rich manipulation tasks. Furthermore, participants significantly preferred our system over comparative more direct interfaces, demonstrating the potential of our approach for enabling end users to effectively perform remote robot programming.
The Clinical Value of Pulmonary Rehabilitation in Reducing Postoperative Complications and Mortality of Lung Cancer Resection: A Systematic Review and Meta-Analysis
Authors: Mao Xiaowei , Ni Yiqian , Niu Yanjie , Jiang Liyan
Published: September 2021

Background: Pulmonary rehabilitation is one meaningful way of improving exercise tolerance and pulmonary function. Thus, it may reduce the postoperative complications and mortality of pulmonary resection. Hence, we refreshed the data and conducted this systemic analysis.

Method: We searched Pubmed, Web of Science, and EMBASE using “lung OR pulmonary” AND “operation OR resection OR surgery” AND “rehabilitation or exercise.” The cut-off date was September 30, 2020. The publications were filtrated, and data were extracted from all selected studies by two reviewers. Review Manger 5.1 and the fixed or random regression model were used for calculating the pooled odds ratio (OR).

Result: Finally, 13 publications were enrolled in this study. Among them, five publications reported mortality, nine reported postoperative complications, and seven reported postoperative pulmonary complications. The pooled OR of mortality was 1.32 [95% confidence interval (CI): 0.54–3.23] for the pulmonary rehabilitation group, the pooled OR of postoperative complications was 0.62 (95% CI: 0.49–0.79) for the pulmonary rehabilitation group, and the pooled OR of postoperative pulmonary complications was 0.39 (95% CI: 0.27–0.56) for the pulmonary rehabilitation group. Subgroup analysis revealed the perioperative pulmonary rehabilitation was the most important part.

Conclusion: Pulmonary rehabilitation may not affect the mortality of pulmonary resection patients, however, it could decrease the number of postoperative complications, especially pulmonary complications. Perioperative pulmonary rehabilitation was the most important part of the program.
Probiotics Isolated From Animals in Northwest China Improve the Intestinal Performance of Mice
Authors: Li Yingying , Jia Dan , Wang Jiahui , Li Hehai , Yin Xijuan , Liu Junlong , Wang Jinming , Guan Guiquan , Luo Jianxun , Yin Hong , Xiao Sa , Li Youquan
Published: September 2021

Antibiotic resistance is an increasingly prevalent problem worldwide. Probiotics are live microorganisms that provide health benefits to human beings and animals and also antimicrobial activity against pathogens and might be an antibiotic alternative. The gastrointestinal tract of animals can be a suitable source of finding novel antimicrobial agents, where the vast majority of gut microbes inhabit and a plurality of antimicrobial producers exhibit either a wide or narrow spectrum. Animals that live in Northwest China might possess a special commensal community in the gut. Therefore, the purpose of this study was to assess the effects of three probiotic strains (including Lactobacillus salivarius ZLP-4b from swine, Lactobacillus plantarum FBL-3a from beef cattle, and Bacillus velezensis JT3-1 from yak), which were isolated from livestock in this area, on the overall growth performance, immune function, and gut microbiota of mice. The results showed that the L. salivarius ZLP-4b group not only improved the growth performance but also amended the intestinal mucosa morphology of mice. Furthermore, the supplementation of L. plantarum FBL-3a and L. salivarius ZLP-4b strains significantly increased the content of anti-inflammatory cytokines IL-4 and IL-10 but decreased the pro-inflammatory factor IL-17A. The levels of pro-inflammatory factors IL-6, IL-17A, and TNF-α were also decreased by the B. velezensis JT3-1 group pretreatment. The 16S rDNA sequence results showed that the probiotic administration could increase the proportion of Firmicutes/Bacteroidetes intestinal microbes in mice. Furthermore, the relative abundance of Lactobacillus was boosted in the JT3-1- and ZLP-4b-treated groups, and that of opportunistic pathogens (including Proteobacteria and Spirochaetes) was diminished in all treated groups compared with the control group. In conclusion, B. velezensis JT3-1 and L. salivarius ZLP-4b supplementation enhanced the overall performance, intestinal epithelial mucosal integrity, and immune-related cytokines and regulated the intestinal microbiota in mice.
Rough Estimator Based Asynchronous Distributed Super Points Detection on High Speed Network Edge
Authors: Xu Jie , Ding Wei
Published: September 2021

Super points detection plays an important role in network research and application. With the increase of network scale, distributed super points detection has become a hot research topic. The key point of super points detection in a multi-node distributed environment is how to reduce communication overhead. Therefore, this paper proposes a three-stage communication algorithm to detect super points in a distributed environment, Rough Estimator based Asynchronous Distributed super points detection algorithm (READ). READ uses a lightweight estimator, the Rough Estimator (RE), which is fast in computation and takes less memory to generate candidate super points. Meanwhile, the famous Linear Estimator (LE) is applied to accurately estimate the cardinality of each candidate super point, so as to detect the super point correctly. In READ, each node scans IP address pairs asynchronously. When reaching the time window boundary, READ starts three-stage communication to detect the super point. This paper proves that the accuracy of READ in a distributed environment is no less than that in the single-node environment. Four groups of 10 Gb/s and 40 Gb/s real-world high-speed network traffic are used to test READ. The experimental results show that READ not only has high accuracy in a distributed environment, but also has less than 5% of communication burden compared with existing algorithms.
Short Communication: Optimally Solving the Unit-Demand Envy-Free Pricing Problem with Metric Substitutability in Cubic Time
Authors: Salvatierra Marcos M. , Salvatierra Mario , Colonna Juan G.
Published: September 2021

In general, the unit-demand envy-free pricing problem has proven to be APX-hard, but some special cases can be optimally solved in polynomial time. When substitution costs that form a metric space are included, the problem can be solved in O(n4) time, and when the number of consumers is equal to the number of items—all with a single copy so that each consumer buys an item—a O(n3) time method is presented to solve it. This work shows that the first case has similarities with the second, and, by exploiting the structural properties of the costs set, it presents a O(n2) time algorithm for solving it when a competitive equilibrium is considered or a O(n3) time algorithm for more general scenarios. The methods are based on a dynamic programming strategy, which simplifies the calculations of the shortest paths in a network; this simplification is usually adopted in the second case. The theoretical results obtained provide efficiency in the search for optimal solutions to specific revenue management problems.
Continuous Control Strategy of Planar 3-Linkage Underactuated Manipulator Based on Broad Neural Network
Authors: Chen Siyu , Wang Yawu , Zhang Pan , Su Chun-Yi
Published: September 2021

For the position control of a planar 3-linkage underactuated manipulator (PTUM) with a passive first linkage, a continuous control strategy is developed in this paper. In particular, a broad neural network (BNN)-based model is first established to accurately describe the motion coupling relationship between the passive linkage and the second linkage. Based on this model, by using the particle swarm optimization algorithm, the target angles of all linkages are calculated combining the start states of all linkages and the target position of the PTUM. Then, the target angles of the active linkages are directly achieved by their respective actuators, and that of the passive linkage is also achieved by the rotation of the second linkage. By carrying out several experiments, the effectiveness of the above strategy is verified.
Permanent Bed Width Has Little Effect on Crop Yield under Rainfed and Irrigated Conditions across Central Mexico
Authors: Saldivia-Tejeda Abel , Fonteyne Simon , Guan Taiyu , Verhulst Nele
Published: September 2021

In Mexico, conservation agriculture has been mainly implemented using permanent beds, where the top of the raised beds is not tilled, which allows them to obtain the benefits of conservation agriculture for yield and soil quality. However, narrow (0.75–0.80 m width) and wide (1.50–1.60 m width) beds are commonly implemented without scientific evidence available as to whether the width of the beds affects crop yields. The objective of our study was therefore to evaluate two types of permanent beds, in maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) production, in various agro-ecological regions of Mexico. The study included nine sites, of which six were rainfed and three had irrigation. Bed width did not significantly affect crop yield. Therefore, farmers can choose the bed width that best meets their practical needs. Some practical considerations include mechanical weeding (more access in narrow beds), fuel use (lower for reshaping wide beds), irrigation water use (in wide beds similar to irrigating alternate furrows in narrow beds), and residue management (option to concentrate residue in windrows at center of wide beds). Soil texture can also affect this choice, because it affects water infiltration and retention.
Remote Sensing for Palmer Amaranth (Amaranthus palmeri S. Wats.) Detection in Soybean (Glycine max (L.) Merr.)
Authors: Sanders John T. , Jones Eric A. L. , Austin Robert , Roberson Gary T. , Richardson Robert J. , Everman Wesley J.
Published: September 2021

Field studies were conducted in 2016 and 2017 to determine if multispectral imagery collected from an unmanned aerial vehicle (UAV) equipped with a five-band sensor could successfully identify Palmer amaranth (Amaranthus palmeri) infestations of various densities growing among soybeans (Glycine max [L.] Merr.). The multispectral sensor captures imagery from five wavebands: 475 (blue), 560 (green), 668 (red), 840 (near infrared [NIR]), and 717 nm (red-edge). Image analysis was performed to examine the spectral properties of discrete Palmer amaranth and soybean plants at various weed densities using these wavebands. Additionally, imagery was subjected to supervised classification to evaluate the usefulness of classification as a tool to differentiate the two species in a field setting. Date was a significant factor influencing the spectral reflectance values of the Palmer amaranth densities. The effects of altitude on reflectance were less clear and were dependent on band and density being evaluated. The near infrared (NIR) waveband offered the best resolution in separating Palmer amaranth densities. Spectral separability in the other wavebands was less defined, although low weed densities were consistently able to be discriminated from high densities. Palmer amaranth and soybean were found to be spectrally distinct regardless of imaging date, weed density, or waveband. Soybean exhibited overall lower reflectance intensity than Palmer amaranth across all wavebands. The reflectance of both species within blue, green, red, and red-edge wavebands declined as the season progressed, while reflectance in NIR increased. Near infrared and red-edge wavebands were shown to be the most useful for species discrimination and maintained their utility at most weed densities. Palmer amaranth weed densities were found to be spectrally distinct from one another in all wavebands, with greatest distinction when using the red, NIR and red-edge wavebands. Supervised classification in a two-class system was consistently able to discriminate between Palmer amaranth and soybean with at least 80% overall accuracy. The incorporation of a weed density component into these classifications introduced an error of 65% or greater into these classifications. Reducing the number of classes in a supervised classification system could improve the accuracy of discriminating between Palmer amaranth and soybean.
Infection of the Western Flower Thrips, Frankliniella occidentalis, by the Insect Pathogenic Fungus Beauveria bassiana
Authors: Zhang Zhijian , Zheng Changying , Keyhani Nemat O. , Gao Yulin , Wang Junping
Published: September 2021

The western flower thrips, Frankliniella occidentalis, is an aggressive agricultural insect pest causing significant damage to a wide range of fruit, vegetable, and ornamental crops. Beauveria bassiana is a broad-host-range entomopathogenic fungus capable of infecting and killing F. occidentalis. Infection of thrips by B. bassiana strain BbYT12 using a concentration of 1 × 108 conidia/mL resulted in 81.48% mortality in adults in 6 d (LT50 = 90 ± 15.1 h). Scanning electron microscopy of the infection process revealed preferential adhesion and germination of fungal spores to inter-segmental folds or grooves on the insect body surface with penetrating germlings and extended hyphae visualized during the initial stages of infection (6–24 h). Histological analyses showed the appearance of in vivo hyphal bodies in sagittal sections and the fat body as early as 24 h post-infection. Within 72 h, hyphal bodies and hyphae could be found throughout the infected organism including in the midgut, Malphigian tubules, alimentary canal, ovarioles (in females), and an extended hyphal network could be seen on insect cadavers (>72 h post-infection). Real-time RT-PCR analyses of the expression of select genes implicated in virulence including the Pr1 protease, beauvericin synthase, involved in the production of the secondary metabolite beauvericin, two cytochrome P450 monooxygenases implicated in cuticular hydrocarbon degradation, two multidrug efflux proteins, a perilipin involved in lipid storage, and the Hog1 MAP kinase and protein kinase A signaling factors revealed discrete patterns of infection-time dependent expression. These data provide basic insights into the process of B. bassiana infection of F. occidentalis.
Genetic Analysis of Yield and Quality Characteristics in Provitamin A Hybrid Cassava Families in Ghana
Authors: Boakye Peprah Bright , Parkes Elizabeth Yaa , Kulakow Peter , van Biljon Angeline , Labuschagne Maryke Tine
Published: September 2021

Cassava is the most widely cultivated and consumed crop in Ghana. Malnutrition is endemic in cassava-producing regions of Africa, partly due to the low micronutrient content of this crop. The aim of this study was to generate genetic information on characteristics such as total carotenoid content, dry matter content, root weight and number, and cassava mosaic disease (CMD), and their possible combination in cassava clones, using a North Carolina II breeding scheme. Five genetically diverse yellow-fleshed clones at advanced selection stages, with CMD resistance, were used as females and two high dry matter content white-fleshed clones, selected from farmers’ fields in Ghana, were used as males. Ten F1 families were generated, and evaluated at two locations in Ghana. General combining ability (GCA) mean squares were larger than specific combining ability (SCA) mean squares for harvest index, CMD, and carotenoid content, indicating additive genetic effects. The positive significant correlations that were observed between pulp color and carotenoid content; carotenoid content and CMD; pulp color and CMD; and pulp color and cortex color, make the screening of large numbers of progenies possible in the cassava breeding program. This could allow breeders to combine carotenoid content and CMD at the early breeding stages through visual assessment of pulp color and CMD symptoms. Large numbers of genotypes can be evaluated and a few can be selected to be quantified for carotenoid content at later stages of the breeding program, to save costs. One of the parents (P6), showed positive GCA effects for carotenoid content, dry matter content, CMD and storage root weight, hence could be used as a parent to generate clones that combine carotenoid content and dry matter content.
Response of Two Local Common Bean Ecotypes of “Fagioli di Sarconi” PGI (Phaseolus vulgaris L.) to Seed-Borne Pathogens and Environmental Change
Authors: Vitti Antonella , Bevilacqua Vincenzo , Logozzo Giuseppina , Marzario Stefania , Gioia Tania , Nuzzaci Maria
Published: September 2021

Among foods protected by the European Union with the PGI (Protected Geographical Indication) mark, several ecotypes of “Fagioli di Sarconi” common beans, typical legumes of Basilicata Region, are included. This work aimed to conduct a survey of seed-borne pathogens isolated from “Ciuoto” and “Cannellino rosso”, ecotypes of “Fagioli di Sarconi” common beans, in two years and cultivation areas of the National Park of the Agri Valley, for identifying resistant and climatic changes well-adapted genotypes. Three validated methods were used for the seed-borne pathogens screening. Eighteen fungi were differently found for ecotype and year of observation by the washing test. Saprophyte contaminants pathogens isolated and detected by the blotter test were strongly reduced by 1% sodium hypochlorite treatment. Using the between paper test, specific for detecting Colletotrichum lindemuthianum, the presence of this pathogen for both ecotypes, years and cultivation areas, and also some bacteria were individuated. Therefore, area-, environment- and ecotype-dependent differences were revealed, probably also caused by a different polyphenolic content and thickness of integument of two ecotypes. This study represents a baseline information for further studies, development of forecasting models and management of seed-borne diseases associated with common beans.
Assessment of the Physiological Condition of Spring Barley Plants in Conditions of Increased Soil Salinity
Authors: Tobiasz-Salach Renata , Stadnik Barbara , Migut Dagmara
Published: September 2021

Soil salinity is one of the most important environmental factors threatening agriculture on a global level. Plants show differences in resistance to salt stress, both in terms of species and variety. The aim of the study was to determine the effect of salt stress on photosynthetic efficiency and the activity of plants of two barley varietie—KWS Irina and RGT Planet. Plants grown in a pot experiment were subjected to soil treatment with sodium chloride (NaCl) at concentrations of 0, 50, 100, and 150 mmol∙(dm3)−1. Measurements were made four times at intervals of 7, 14, 21, and 28 days after the application of NaCl. The relative chlorophyll content in leaves (CCl) and selected chlorophyll fluorescence parameters (Fv/Fm, Fv/F0, and PI) and gas exchange parameters (PN, E, gs, and Ci) were assessed. In the final stage of the experiment, a visual assessment of the plants’ condition was carried out and the amount of fresh mass (FM) of the above-ground part was determined. The content of sodium and potassium in the vegetative parts of plants was also analysed. Salinity significantly influenced the values of the measured parameters in both of the tested barley genotypes. High salt concentrations in the soil at levels of 100 and 150 mmol NaCl (dm3)−1 negatively affected the growth and development of plants by disturbing the process of photosynthesis and other plant gas exchange parameters. The antagonistic effect of sodium in relation to potassium resulted in a decrease in the K+ content in the plants, along with an increase in the salinity level.
Strategic Identification of New Genetic Diversity to Expand Lentil (Lens culinaris Medik.) Production (Using Nepal as an Example)
Authors: Neupane Sandesh , Dhakal Rajeev , Wright Derek M. , Shrestha Deny K. , Dhakal Bishnu , Bett Kirstin E.
Published: September 2021

Although lentil has a long history of cultivation, cultivars rely on a narrow genetic base, indicating room for broadening the diversity. Two field experiments were conducted at Bardiya, Nepal, during winter 2016 and 2017, with 324 diverse lentil genotypes obtained from genebanks and breeding programs around the world. Phenological traits related to adaptation, particularly days to flower, were assessed. A photothermal model was used to predict days to flower in new environments to identify genotypes that may be suitable for additional growing regions in Nepal, allowing for the expansion of the production area. Many putatively adapted genotypes were identified for terai, mid-hill, and high-hill growing regions. The list includes large-seeded or yellow cotyledon lines, representing new market classes of lentils for Nepal.
Challenges for Agriculture through Industry 4.0
Authors: Bernhardt Heinz , Bozkurt Mehmet , Brunsch Reiner , Colangelo Eduardo , Herrmann Andreas , Horstmann Jan , Kraft Martin , Marquering Johannes , Steckel Thilo , Tapken Heiko , Weltzien Cornelia , Westerkamp Clemens
Published: September 2021

Industry 4.0 is currently considered the structural implementation of networked and cooperative digitalisation and the next step in technological and social development. The aim of this paper is to examine how these structures are also suitable for agriculture and whether there are already approaches to this. Therefore, the main aspects of Industry 4.0 will be analysed and compared with agricultural examples from arable farming and livestock farming. The study shows that the approaches of Industry 4.0 are also useful for agriculture. However, they must be adapted to agriculture, as it has a different basic structure. As in industry, it is also evident in agriculture that there is still a need for action in the organisational and technical networking of systems.
Automatic Recognition of Fish Behavior with a Fusion of RGB and Optical Flow Data Based on Deep Learning
Authors: Wang Guangxu , Muhammad Akhter , Liu Chang , Du Ling , Li Daoliang
Published: September 2021

The rapid and precise recognition of fish behavior is critical in perceiving health and welfare by allowing farmers to make informed management decisions on recirculating aquaculture systems while reducing labor. The conventional recognition methods are to obtain movement information by implanting sensors on the skin or in the body of the fish, which can affect the normal behavior and welfare of the fish. We present a novel nondestructive method with spatiotemporal and motion information based on deep learning for real-time recognition of fish schools’ behavior. In this work, a dual-stream 3D convolutional neural network (DSC3D) was proposed for the recognition of five behavior states of fish schools, including feeding, hypoxia, hypothermia, frightening and normal behavior. This DSC3D combines spatiotemporal features and motion features by using FlowNet2 and 3D convolutional neural networks and shows significant results suitable for industrial applications in automatic monitoring of fish behavior, with an average accuracy rate of 95.79%. The model evaluation results on the test dataset further demonstrated that our proposed method could be used as an effective tool for the intelligent perception of fish health status.
Effect of Calving Difficulties and Calf Mortality on Functional Longevity in Polish Holstein-Friesian Cows
Authors: Morek-Kopeć Małgorzata , Zarnecki Andrzej , Ptak Ewa , Otwinowska-Mindur Agnieszka
Published: September 2021

Longevity is one of the functional traits that considerably affect dairy herd profitability. A Weibull proportional hazards model was used to evaluate the impact of difficult calvings and calf stillbirths on cow functional longevity, defined as length of productive life corrected for milk production. The data for analysis comprised calving ease and calf mortality scores of 2,163,426 calvings, 34.4% of which came from primiparous cows. The percentage of male calves was 53.4%. Calving ease was scored as “without assistance” (34.44%), “with assistance” (62.03%), “difficult—hard pull” (3.39%), and “very difficult, including caesarean section” (0.14%). Calf mortality scores were “live born” (94.21%) and “stillborn or died within 24 h” (5.79%). The Weibull proportional hazards model included classes of calving ease or calf mortality scores × parity (1, ≥2) × sex of calf as time-dependent fixed effect. The model also included time-dependent fixed effects of year × season, parity × stage of lactation, annual change in herd size, fat yield and protein yield, time-independent fixed effect of age at first calving, and time dependent random herd × year × season. In first-parity cows, very difficult birth of a bull or heifer increased the relative risk of culling, respectively, 2.18 or 1.26 times as compared with calving without assistance. In later parities, the relative risk of culling related to very difficult calving was 2.0 times (for male calves) and 1.33 times (for female calves) higher than the relative risk of culling associated with calving without assistance. Calf mortality showed a negative impact on longevity in both heifers and cows. First-parity stillbirth increased the relative risk of culling depending on sex of calf by 18% in females and by 15% in males; in later parities the increase of the relative risk of culling was lower (by 7% for females, 9% for males). Difficult calvings and their consequences, especially in primiparous cows, may negatively influence dairy herd profitability by reducing the length of cows’ productive life.
Seroprevalence and Risk Factors of Anaplasma spp. in German Small Ruminant Flocks
Authors: Rubel Wiebke , Schoneberg Clara , Wolf Annika , Ganter Martin , Bauer Benjamin Ulrich
Published: September 2021

Knowledge about the distribution of Anaplasma spp. in small ruminants from Germany is limited. Therefore, serum samples were examined from 71 small ruminant flocks (2731 sheep, 447 goats) located in the five German federal states: Schleswig-Holstein (SH), Lower Saxony (LS), North Rhine-Westphalia (NRW), Baden-Wuerttemberg (BW) and Bavaria (BAV). Antibodies to Anaplasma spp. were determined by a cELISA based on the MSP5 antigen. A risk factor analysis at animal and flock level was also performed. Antibodies to Anaplasma spp. were detected in 70/71 flocks without significant difference in the intra-flock prevalence (IFP) between the federal states. The mean antibody levels from sheep were significantly lower in northern Germany (LS, SH) compared to west (NRW) and south Germany (BW, BAV). Sheep had a 2.5-fold higher risk of being seropositive than goats. Females and older animals (>2 years) were more likely to have antibodies to Anaplasma spp. in one third and one quarter of cases, respectively. Flocks used for landscape conservation had a five times higher risk of acquiring an IFP greater than 20%. Cats and dogs on the farms increased the probability for small ruminant flocks to have an IFP of above 20% 10-fold and 166-fold, respectively. Further studies are necessary to assess the impact of Anaplasma species on the health of small ruminants in Germany.
Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats
Authors: He Yanyu , Liu Xiu , De Jie , Kang Saihong , Munday John S.
Published: September 2021

This experiment compared secondary hair follicles (SFs) in Tibetan cashmere goats from two different steppes that were at different altitudes and had different temperatures. Twenty-four 2-year-old goats were studied. Twelve goats were from Rikaze in Tibet which is at an altitude of above 5000 m with an average temperature of 0 °C. The other 12 studied goats were from Huan County of Gansu Province which is around 2000 m above sea level with an average temperature of 9.2 °C. The structural features of SFs were assessed using light microscopy and transmission electron microscopy. The presence of HIF-1a, HIF-2a, HIF-3a, HSP27, and HOXC13 proteins was studied using immunohistochemistry and immunofluorescence. Light and electron microscopy revealed that the SFs of the Tibetan cashmere goats that lived in the Rikaze Steppe were in the proanagen stage in May. However, the SFs of the goats from the lower warmer Huan County were in the anagen stage at the same time. Immunohistochemistry revealed intense immunostaining for HIF-1a protein in the inner root sheath (IRS) and hair shaft (HS); immunostaining against HIF-2a in the outer root sheath (ORS) and IRS; HIF-3a protein immunostaining in the ORS; HSP27 immunostaining in the ORS, IRS, and HS; and HOXC13 immunostaining in the ORS and HS. HIF-1a protein expression in the IRS and HS was higher than the expression in the ORS (p < 0.05) while the expression of HIF-2a protein was higher in the ORS and IRS than the HS (p < 0.05). The expression of HIF-3a protein was higher in the ORS than in the IRS (p < 0.05). Expression of HOXC13 protein was higher in the ORS than in the IRS and HS (p < 0.05). Immunostaining of HIF-1a, HIF-2a, and HSP27 protein was significantly higher in SFs from cashmere goats from Rikaze than in goats from Huan (p < 0.05). In contrast, HOX13 protein immunostaining was significantly higher in cashmere goats from Huan than from Rikaze (p < 0.05). Significant differences were observed in the SFs of cashmere goats from two locations that differ in altitude and temperature. This suggests the differences in the secondary hair follicles could be due to the hypoxia and lower temperatures experienced by the goats in Rikaze. These results are useful in understanding how altitude and temperature influence SF development. Hair produced by the SFs are used for down fiber. Therefore, understanding of the factors that influence SF development will allow the production and harvest of these valuable fibers to be maximized.
Investigation of the Food-Transmitted Parasites Trichinella spp. and Alaria spp. in Wild Boars in Greece by Classical and Molecular Methods and Development of a Novel Real-Time PCR for Alaria spp. Detection
Authors: Dimzas Dimitris , Chassalevris Taxiarchis , Ozolina Zanda , Dovas Chrysostomos I. , Diakou Anastasia
Published: September 2021

Foodborne parasitic diseases represent a major threat to public health. Trichinellosis, caused by the nematode parasite Trichinella spp., is one of the most important foodborne diseases, while alariosis, caused by the trematode parasite Alaria spp., is less common in humans, and rare cases have been reported only in the USA and Canada. Both parasites can infect humans via the consumption of raw or undercooked wild boar meat. In order to investigate the prevalence of these parasites in wild boar meat in Greece, samples from the diaphragm pillars and the region of the mandibular angle from 128 wild boars, hunted in Greece, were collected. The samples were examined by classical parasitological (compression, artificial digestion, and Alaria spp. migration) and by molecular (real-time PCR) methods. For Trichinella spp. an existent real-time PCR detecting all species likely to be present in Greece was applied, while for Alaria spp. a real-time PCR was developed, employing an LNA TaqMan probe targeting the large subunit ribosomal RNA gene. All examined wild boar samples from Greece resulted negative for Trichinella and Alaria species, indicating a low prevalence of infection in the examined population. The novel real-time PCR for Alaria spp. has 81.5% amplification efficiency and is able to detect 0.12 larvae per 50 g of tissue and could be utilized as a complementary to AMT diagnostic tool in surveillance.
Objective Video-Based Assessment of ADHD-Like Canine Behavior Using Machine Learning
Authors: Fux Asaf , Zamansky Anna , Bleuer-Elsner Stephane , van der Linden Dirk , Sinitca Aleksandr , Romanov Sergey , Kaplun Dmitrii
Published: September 2021

Canine ADHD-like behavior is a behavioral problem that often compromises dogs’ well-being, as well as the quality of life of their owners; early diagnosis and clinical intervention are often critical for successful treatment, which usually involves medication and/or behavioral modification. Diagnosis mainly relies on owner reports and some assessment scales, which are subject to subjectivity. This study is the first to propose an objective method for automated assessment of ADHD-like behavior based on video taken in a consultation room. We trained a machine learning classifier to differentiate between dogs clinically treated in the context of ADHD-like behavior and health control group with 81% accuracy; we then used its output to score the degree of exhibited ADHD-like behavior. In a preliminary evaluation in clinical context, in 8 out of 11 patients receiving medical treatment to treat excessive ADHD-like behavior, H-score was reduced. We further discuss the potential applications of the provided artifacts in clinical settings, based on feedback on H-score received from a focus group of four behavior experts.
Genetic Variations and Haplotypic Diversity in the Myostatin Gene of Different Cattle Breeds in Russia
Authors: Konovalova Elena , Romanenkova Olga , Zimina Anna , Volkova Valeria , Sermyagin Alexander
Published: September 2021

The myostatin gene (MSTN) in cattle has a number of polymorphisms associated with increased muscle mass. The aim of the current study was to determine the haplotype frequencies of F94L and nt821(del11) MSTN polymorphisms among cattle bred for meat in Russia, using DNA analysis. Using the earlier created test systems based on the AS-PCR and PCR-RFLP methods, six populations of Aberdeen Angus (n = 684), two populations of Limousin (n = 54), one population of Simmental (n = 55), and one population of Belgian Blue (n = 137) belonging to Russian farms were genotyped on nt821(del11) and F94LMSTN polymorphisms. The animal carriers of the mutant allele of nt821(del11)MSTN associated with the double-muscling genetic defect were found in one Aberdeen Angus population at a frequency of 2.18%, but were not found in the Limousin and Simmental populations. However, 100% of the Belgian Blue population were heterozygous carriers of nt821(del11)MSTN. The frequencies of the A allele F94LMSTN desirable for productivity traits in the Limousin populations were the highest and accounted for 0.97 and 1 in populations one and two, while in the Aberdeen Angus, Simmental, and Belgian Blue populations, these figures were considerably lower at 0.04–0.08, depending on the population. The obtained data show the high genetic potential of Russian beef cattle, and facilitate an improvement in meat productivity by preserving the health of animals.
The Tongue in Three Species of Lemurs: Flower and Nectar Feeding Adaptations
Authors: Pastor Juan Francisco , Muchlinski Magdalena Natalia , Potau Josep Maria , Casado Aroa , García-Mesa Yolanda , Vega Jose Antonio , Cabo Roberto
Published: September 2021

The mobility of the primate tongue allows for the manipulation of food, but, in addition, houses both general sensory afferents and special sensory end organs. Taste buds can be found across the tongue, but the ones found within the fungiform papillae on the anterior two thirds of the tongue are the first gustatory structures to come into contact with food, and are critical in making food ingestion decisions. Comparative studies of both the macro and micro anatomy in primates are sparse and incomplete, yet there is evidence that gustatory adaptation exists in several primate taxa. One is the distally feathered tongues observed in non-destructive nectar feeders, such as Eulemur rubriventer. We compare both the macro and micro anatomy of three lemurid species who died of natural causes in captivity. We included the following two non-destructive nectar feeders: Varecia variegata and Eulemur macaco, and the following destructive flower feeder: Lemur catta. Strepsirrhines and tarsiers are unique among primates, because they possess a sublingua, which is an anatomical structure that is located below the tongue. We include a microanatomical description of both the tongue and sublingua, which were accomplished using hematoxylin–eosin and Masson trichrome stains, and scanning electron microscopy. We found differences in the size, shape, and distribution of fungiform papillae, and differences in the morphology of conical papillae surrounding the circumvallate ones in all three species. Most notably, large distinct papillae were present at the tip of the tongue in nectar-feeding species. In addition, histological images of the ventro-apical portion of the tongue displayed that it houses an encapsulated structure, but only in Lemur catta case such structure presents cartilage inside. The presence of an encapsulated structure, coupled with the shared morphological traits associated with the sublingua and the tongue tip in Varecia variegata and Eulemur macaco, point to possible feeding adaptations that facilitate non-destructive flower feeding in these two lemurids.
Systematic Review of the Interaction between Nutrition and Immunity in Livestock: Effect of Dietary Supplementation with Synthetic Amino Acids
Authors: Montout Laura , Poullet Nausicaa , Bambou Jean-Christophe
Published: September 2021

Infectious diseases represent one of the most critical threats to animal production worldwide. Due to the rise of pathogen resistance and consumer concern about chemical-free and environmentally friendly productions, the use of antimicrobials drugs is no longer desirable. The close relationship between nutrition and infection has led to numerous studies about livestock. The impact of feeding strategies, including synthetic amino acid supplementation, on host response to various infections has been investigated in different livestock animals. This systematic review provides a synthesis of the experimental studies on the interactions between synthetic amino acid supplementation and immune response to infectious diseases in livestock. Following PRISMA guidelines, quantitative research was conducted using two literature databases, PubMed and Web of Science. The eligibility criteria for the research articles were: (1) the host is a livestock animal; (2) the supplementation with at least one synthetic amino acid; (3) at least one mediator of immunity is measured; (4) at least one production trait is measured. Data were extracted from 58 selected studies. Articles on poultry were the most numerous; few contained experiments using ruminants and pigs. Most of the authors hypothesized that synthetic amino acid supplementation would particularly improve the animals’ immune response against intracellular pathogens. An increase in T and natural killer lymphocytes and macrophages activation, intracellular redox state, lymphocytes proliferation and antibodies production were the most described immune mechanisms associated with synthetic amino acid supplementation. Most of the selected studies focused on three amino acids (methionine, threonine and arginine), all of which are associated with a significant improvement of the host immune response. The use of synthetic amino acid supplementation appears as an encouraging perspective for livestock infectious disease management, and research must concentrate on more analytical studies using these three amino acids.
Addendum: Pongrácz et al. Grumpy Dogs Are Smart Learners—The Association between Dog–Owner Relationship and Dogs’ Performance in a Social Learning Task. Animals 2021, 11, 961
Authors: Pongrácz Péter , Rieger Gabriella , Vékony Kata
Published: September 2021

The authors would like to make an addendum to their published paper [...]
Elephant Endotheliotropic Herpesvirus Impact in the European Asian Elephant (Elephas maximus) Population: Are Hereditability and Zoo-Associated Factors Linked with Mortality?
Authors: Jesus Sónia A. , Doherr Marcus G. , Hildebrandt Thomas B.
Published: September 2021

EEHV is a ubiquitous virus, which most likely has co-evolved with elephants and is shed by healthy individuals and maintained in the herds. Yet, the factors determining calf susceptibility to the virus remain unknown. Here, we explored the impact of EEHV-HD in the European captive Asian elephant population in a retrospective statistical study spanning the last 35 years. We show that EEHV-HD was implicated in more than half of all deaths recorded in calves older than one months old. Moreover, the median age across EEHV-HD fatalities was significantly lower compared to other death causes. Finally, we investigated if heredity and zoo-associated factors could be linked to a higher susceptibility of calves to this disease. We used a univariable logistic regression model to evaluate if either fathers, mothers, or zoos could, separately, be considered as risk factors to the development of the disease. Afterwards, we used a two multivariable model, combining: (1) fathers and zoos, and (2) mothers and zoos. Overall, we found that two fathers, one mother, and four zoos had three or more times higher risk of their calves becoming sick when compared to all others, pointing us to the presence of a management or environmental element, which can have paternal and maternal influence and leads to calf susceptibility or resistance to EEHV-HD.
Genetic Comparison of ESBL-Producing Escherichia coli from Workers and Pigs at Vietnamese Pig Farms
Authors: Truong Duong Thi Quy , Hounmanou Yaovi Mahuton Gildas , Dang Son Thi Thanh , Olsen John Elmerdahl , Truong Giang Thi Huong , Tran Nhat Thi , Scheutz Flemming , Dalsgaard Anders
Published: September 2021

We analyzed and compared genomes of Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli from pigs and pig farm workers at 116 farms in Vietnam. Analyses revealed the presence of blaCTX-M-55, blaCTX-M-27, blaCTX-M-15, blaCTX-M-14, blaCTX-M-3, blaCTX-M-65, blaCTX-M-24, blaDHA-1, and blaCMY2 in both hosts. Most strains from pigs contained quinolones (qnr) and colistin resistance genes (mcr-1 and mcr-3). Isolates predominantly harbored more than one plasmid replicon and some harbored plasmid replicons on the same contigs as the ESBL genes. Five strains from farm workers of ST38 (2), ST69 (1), and ST1722 (2) were classified as either uropathogenic E. coli (UPECHM)/extraintestinal pathogenic E. coli (ExPECJJ) or UPECHM, and the remaining were genetically distinct commensals. A high heterogeneity was found among the ESBL-producing E. coli from pigs and workers, with most isolates belonging to unrelated phylogroups, serogroups, and sequence types with >4046 Single-Nucleotide Polymorphisms-(SNPs). In comparing the genomes of pig isolates to those from humans, it appeared that ESBL-producing E. coli in workers did not predominantly originate from pigs but were rather host-specific. Nevertheless, the occurrence of ESBL-producing E. coli carrying plasmid-mediated colistin and quinolone resistance genes in pigs could represent a potential source for horizontal transmission to humans through food rather than direct contact.
Saccharomyces cerevisiae Rhodanese RDL2 Uses the Arg Residue of the Active-Site Loop for Thiosulfate Decomposition
Authors: Wang Qingda , Li Huanjie , Xia Yongzhen , Xun Luying , Liu Huaiwei
Published: September 2021

Persulfide, polysulfide and thiosulfate are examples of sulfane sulfur containing chemicals that play multiple functions in biological systems. Rhodaneses are widely present in all three kingdoms of life, which catalyze sulfur transfer among these sulfane sulfur-containing chemicals. The mechanism of how rhodaneses function is not well understood. Saccharomyces cerevisiae rhodanese 2 (RDL2) is involved in mitochondrial biogenesis and cell cycle control. Herein, we report a 2.47 Å resolution structure of RDL2 co-crystallized with thiosulfate (PDB entry: 6K6R). The presence of an extra sulfur atom Sδ, forming a persulfide bond with the Sγ atom of Cys106, was observed. Distinct from the persulfide groups in GlpE (PDB entry:1GMX) and rhobov (PDB entry:1BOI), the persulfide group of RDL2 is located in a peanut-like pocket of the neutral electrostatic field and is far away from positively charged amino acid residues of its active-site loop, suggesting no interaction between them. This finding suggests that the positively charged amino acid residues are not involved in the stabilization of the persulfide group. Activity assays indicate that the Arg111 of the active-site loop is critical for the sulfane sulfur transfer. In vitro assays indicate that Arg propels the thiosulfate decomposition. Thus, we propose that Arg can offer a hydrogen bond-rich, acidic-like microenvironment in RDL2 in which thiosulfate decomposes to release sulfane sulfur. Thr of the active-site loop of rhodaneses has the same functions as Arg. Our proposal may explain the catalyzing mechanism of rhodaneses.
Fursultiamine Prevents Drug-Induced Ototoxicity by Reducing Accumulation of Reactive Oxygen Species in Mouse Cochlea
Authors: Kim Ye-Ri , Kwon Tae-Jun , Kim Un-Kyung , Lee In-Kyu , Lee Kyu-Yup , Baek Jeong-In
Published: September 2021

Drug-induced hearing loss is a major type of acquired sensorineural hearing loss. Cisplatin and aminoglycoside antibiotics have been known to cause ototoxicity, and excessive accumulation of intracellular reactive oxygen species (ROS) are suggested as the common major pathology of cisplatin- and aminoglycoside antibiotics-induced ototoxicity. Fursultiamine, also called thiamine tetrahydrofurfuryl disulfide, is a thiamine disulfide derivative that may have antioxidant effects. To evaluate whether fursultiamine can prevent cisplatin- and kanamycin-induced ototoxicity, we investigated their preventive potential using mouse cochlear explant culture system. Immunofluorescence staining of mouse cochlear hair cells showed that fursultiamine pretreatment reduced cisplatin- and kanamycin-induced damage to both inner and outer hair cells. Fursultiamine attenuated mitochondrial ROS accumulation as evidenced by MitoSOX Red staining and restored mitochondrial membrane potential in a JC-1 assay. In addition, fursultiamine pretreatment reduced active caspase-3 and TUNEL signals after cisplatin or kanamycin treatment, indicating that fursultiamine decreased apoptotic hair cell death. This study is the first to show a protective effect of fursultiamine against cisplatin- and aminoglycoside antibiotics-induced ototoxicity. Our results suggest that fursultiamine could act as an antioxidant and anti-apoptotic agent against mitochondrial oxidative stress.in cochlear hair cells.