bluuebunny/crossref_metadata_2025 huggingface dataset:
Beitrag zum Stoffwechsel des Waschbären
Authors: Neseni Raimund
Published: August 2010

Die durchgeführten Untersuchungen über den Stoffwechsel des Waschbären zeitigten folgende Ergebnisse:

Beim Waschbär wird die Verdaulichkeit der Nährstoffe durch die Zugabe der Zerealien im allgemeinen herabgesetzt. Die Verminderung wird gesteigert durch die Verabreichung der Körner im ungekochten Zustande. Die Verwendung von Futterkuchen ist zwar bequem, aber teuer. Die untersuchten Körnerfrüchte in einer absteigenden Reihe nach ihrer durchschnittlichen Verdaulichkeit aufgestellt, ergeben folgendes Bild: 1. Reis, 2. Mais, 3. Weizen, 4. Hafer und 5. Gerste. Die Kuttel ist dem Rindfleische gegenüber minderwertiger. Als Ballastfutter wäre vor allem Gerste und Hafer zu geben, letzterer aber im geschrotenen, nicht im gequetschten Zustande.

Die Menge des Harnes ist von der Art der Nahrung abhängig und ist bei reiner Fleischkost (FK) am geringsten. Bei dieser ist auch die Chlorausscheidung je Tag sehr niedrig, bei gemischter Kost (GK) dagegen stark erhöht. Die Mengen des Gesamt‐S und der Gesamtschwefelsäuren liegen bei FK höher als bei GK. Von der Gesamtschwefelsäure entfallen durchschnittlich 94,5% auf die Sulfatschwefelsäuren, der Rest auf die Ätherschwefelsäuren. Die geringe Menge dieser erklärt sich aus der geringen bakteriellen Tätigkeit im Darmkanale. Die Menge des Neutral‐S beträgt etwa 15% des Gesamt‐S. Ca und Mg werden nur in geringen Mengen ausgeschieden. Das Verhältnis Ca: Mg ist bei gemischter Rohkost 7: 1, bei gekochten Zerealien 2: 1. Der Gesamt‐N weist bei gemischter Rohkost höhere Werte auf als bei gekochter Kost. In den täglichen Harnmengen steigen die Harnstoffwerte bei animalischer oder gemischter Rohkost stark an.

Bei Futterberechnungen sind folgende Verbrennungswerte beim Waschbär einzusetzen:

Tierisches Eiweiß…… 5,4 Kal.

Pflanzliches Eiweiß…. 4,5 Kal.

N‐freie Extraktstoffe…. 3,6 Kal.

Tierisches Fett…… 8,3 Kal.

Pflanzliches Fett…… 7,4 Kal.

Rohfaser……… 1,3 Kal.

Für wachsende Tiere sind je Kilogramm Lebendgewicht 6–8 g Rohprotein und etwa 100 Kal. zu füttern.
Untersuchungen über einige Mahlabfälle (Haferschälkleie, Haferschalen, Weizen‐ und Roggenkeime) an Schafen, Schweinen und Hühnern und deren Einfluß auf den Säurebasenhaushalt
Authors: Lenkeit W. , Schleinitz M. v. , Lagneau F.
Published: August 2010

Die Haferschälkleie wird durch das Schaf am besten ausgenutzt bei einer Verdaulichkeit der organischen Substanz von 64,89% gegenüber dem Schwein mit 58,31% und dem Huhn mit 52,39%.

Die Haferschalen, die nur an Schafen geprüft wurden, liegen mit der Verdaulichkeit der gesamten Substanz von 37,36 und einem Stärkewert von 24,2 höher als die sonst angegebenen Werte. Das Haferschalenmehl ergab nur eine Verdaulichkeit der organischen Substanz von 31,83%.

Die Weizenkeimkleie (Weizenkeime I) zeigte ebenfalls nach der Verdaulichkeit der organischen Substanz die bessere Verwertung durch den Wiederküuer (93,98%), dann eine geringere durch das Schwein (89,46%) und die niedrigste durch das Huhn (74,79%). Die eigentlichen Weizenkeime (II), die wie die Roggenkeime allein am Huhn untersucht wurden, ergaben einen Gesamtnährstoff von 80,92 und die Roggenkeime einen von 68,76.

In der Stickstoffbilanz ergibt sich eine vermehrte N‐Ausscheidung bei Schwein und Huhn durch die Haferschälkleie und beim Schaf durch die Haferschalen, besonders durch das Haferschalenmehl. Auf die Rolle der Rohfaserausnutzung wird hingewiesen.

Die Basenausscheidung im Harn wird durch die Haferschälkleie bei Schaf und Schwein vermindert, durch Weizenkeime I beim Schaf vermehrt und beim Schwein und Huhn dagegen vermindert. Dieser letztere Unterschied wird diskutiert.
Die Nährwirkung des Haferschrotes beim Schwein 1. Versuchsreihe
Authors: Fingerling† G. , Eisenkolbe P. , Hientzsch Br. , Just M. , Reifgerst K. , Knaut G. , Kunze H.
Published: August 2010

Zusammenfassung der Ergebnisse

Zum Schluß stellen wir nochmals unter Mittelbildung die Ergebnisse zusammen, die unsere Versuche mit den 6 Schweinen gezeitigt haben.

Die ansatzfähige Energie des Haferschrotes wurde verwertet von:

100 g Haferschrot‐Trockensubstanz bewirkten folgenden Ansatz:

Der Stärkewert betrug:

100 g verdaute organische Substanz bewirkten folgenden Ansatz:

Die Wertigkeit betrug:
Beifutter für Milchkühe auf der Weide.
Authors: Haring F. , Kublitz M.
Published: August 2010

Mit insgesamt 21 Kühen in 3 Gruppen werden Kakaoschalen, Sauerkartoffeln und eine vielseitige Kraftfuttermischung mit tierischem Eiweiß und Fett als Beifutter zum Weidegang untersucht. Die Kakaoschalen steigern die Milchfettprozente um maximal 1,0%. Dabei sinkt die Milchmenge um 2,0 kg und mehr. Die Grenze der Aufnahmewilligkeit für Kakaoschalen liegt bei 1810 g je Tier und Tag. Die Beifütterung von Sauerkartoffeln läßt keine deutlichen Einflüsse auf Fettprozent und Milchmenge erkennen. Die vielseitige Kraftfuttermischung wirkt steigernd auf den Milchfettgehalt und die Milchmenge. Schädigende Einflüsse wurden bei keinem Beifutter beobachtet.
Bemerkungen zu der Arbeit von H. Møllgaard: Analyse des Wachstums mittels Differentialgleichungen der Partialprozesse des synthetischen Stoffwechsels
Authors: Hohls H.‐W.
Published: August 2010

Es werden zwei graphische Methoden angegeben, mit deren Hilfe sich die Q‐Werte direkt aus den Kurven des täglichen Gewichtszuwachses oder des relativen täglichen Geewichtszuwachses bestimmen lassen. Ferner wird der Verlauf der Q‐Werte von der Geburt bis zur ßeendigung des Wachstums festgestellt und diskutiert, wieweit neben den Q‐Werten weitere Meßdaten berücksichtigt werden müssen, urn eindeutige Schlüsse aus den Q‐Werten ziehen zu können.
Weitere Untersuchungen über die Beeinflussung des Fettgehaltes der Milch durch Beifütterung während des Weideganges
Authors: Witt M.
Published: August 2010

Der Fettgehalt der Milch schnellt beim übergang von der Stallhaltung zum ganztätigen Weidegang in der ersten Woche empor. In den Wochen danach nimmt er allmählich ab. Nachdem er in der 8. Weidewoche nach einem Rückgang von etwa 0,5% den tiefsten Stand erreicht hat, erholt er sich langsam wiedsr. Dieses Verhalten des Fettgehaltes der Milch ließ sich weder durch eine Beifütterung von kohlenhydrat‐reichen noch von eiweißreichen StE beeinflussen. Es bedarf weiterer sorgfältiger Untersuchungen, um die Gründe für dieses vorübergehende Absinken des Fettgehaltes der Milch zu ermitteln.
Usefulness of catheterless radiotelemetry pH monitoring system to examine the relationship between duodenal acidity and upper gastrointestinal symptoms
Authors: Tanimura Takashi , Adachi Kyoichi , Furuta Kenji , Ohara Shunji , Morita Terumi , Koshino Kenji , Miki Masaharu , Kinoshita Yoshikazu
Published: August 2010

Background and Aim:  To clarify the usefulness of a newly designed method for measuring intraduodenal pH to examine the relationship between duodenal acidity and upper gastrointestinal symptoms during intragastric acid infusion.

Methods:  The study subjects were six healthy volunteers. A Bravo pH capsule with thread fixed to the gastric wall was endoscopically introduced into the second portion of the duodenum, and intraduodenal acidity was measured during intragastric infusion of 300 mL of 0.1 mol/L hydrochloric acid or pure water through an elemental diet tube. The severity of several upper gastrointestinal symptoms were assessed by using a 10‐cm visual analogue scale every 2 min for up to 30 min, and the area under the severity scale‐time curve (cm × min.) were calculated.

Results:  The percentage time during 30 min when the intraduodenal pH was < 4.0 and was significantly greater than during water infusion (61.4 ± 6.1% vs 24.8 ± 6.5%). Several upper gastrointestinal symptoms were observed during acid infusion (acid vs water epigastric heaviness, 29.1 ± 12.0 vs 2.7 ± 1.4; dull epigastric pain, 8.8 ± 4.9 vs 0.7 ± 0.7 cm × min/30 min). Intraduodenal pH below 4.0 was correlated with the severity of dull pain in the stomach (R2 = 0.342, P = 0.044).

Conclusion:  The newly designed intraduodenal pH monitoring by using catheterless radiotelemetry system is useful to examine the relationship between duodenal acidity and upper gastrointestinal symptoms.
ROLE OF THE RETICULAR CELLS DURING MATURATION PROCESS OF THE ERYTHROBLAST
Authors: Seki Masatoshi , Yoneyama Tatsuo , Shirasawa Haruyuki
Published: August 2010

Further electron‐microscopic evidences were produced, by observing the mouse spleen fixed in glutar‐aldehyde fixative, in support of the authors' hypothesis that in the denucleation of the erythroblasts the reticular cells play an active phagocytosing role.

It was pointed out that artefacts, more frequently produced by osmic acid fixation, might possibly have led to erroneous interpretations. On the basis of the findings, various hypotheses for the denucleation mechanism were criticized.
— Scanning Electron Microscopic Observation of Amyloid‐Laden Mouse Liver —
Authors: Takahashi Mutsuo
Published: August 2010

Amyloidosis of liver in mice induced by casein injection was studied by scanning electron microscopy (SEM). In the fractured‐surfaces of the central portion of amyloid nodules, they appeared as chestnut‐bur‐like structures and were composed of numerous thread‐like structures of fasciculated amyloid fibrils and of stick‐like structures of amyloid bundles in three dimensional figure. In the cytoplasm of the Kupffer cell, amyloid bundles were revealed as band‐like bulges. These bulges were projected from the Kupffer cell into the Disse's space and continued with thread‐like structures outside the cytoplasm of Kupffer cells. ACTA PATH. JAP. 27: 809‐822, 1977.
MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN THE GASTRIC MUCOSA OF A/HEJ MICE INJECTED WITH A XENOGENEIC STOMACH ANTIGEN
Authors: Watanabe Hiromitsu , Hirose Fumio , Takizawa Shoichi , Terada Yoritaka , Yumoto Isao Ftokichi
Published: August 2010

During the past 6 years we have experienced a total of 6 cases of malignant lymphomas, which originated primarily in the central nervous system. The most reliable diagnostic criteria will be a “perivascular cuffing” by silver impregnation with further spreading of tumor cells to the periphery, a “starry‐sky” appearance consisting of numerous histiocytes with foamy cytoplasm intermingled with tumor cells, and cytological features of the imprint preparation. Since there is a close histologic similarity to extracerebral malignant lymphomas, we would like to propose that these tumors should be regarded as primary malignant lymphomas of the central nervous system instead of mere reticulum cell sarcoma‐microglioma, and also would like to regard the brain to be not an exceptional site other than visceral lymphomas for occurrence of malignant lymphomas. For the pathogenesis of primary intracranial malignant lymphomas, the hypothesis, proposed by Fbigin, of the possible existence of multipotential stem cells in the brain was discussed.
CHRONIC GASTRITIS OBSERVED FROM A STANDPOINT OF CHRONIC ORGAN INFLAMMATION
Authors: Watanabe Tsunehiko
Published: August 2010

The muco‐muscularis ratio (MMR) of the pyloric antrum was examined in ‘normal’ stomachs and stomachs with chronic gastritis, small chronic gastric ulcers, and duodenal ulcers. The majority of normal stomachs revealed a figure of less than 1 (average 0.803). In chronic gastritis, the MMR was rather high (average 1.127). This fact indicated that the characteristic feature in this condition was atrophy of the muscularis externa rather than that of the mucous membrane. The MMR in stomachs with small chronic ulcers showed no significant difference compared with that of normal samples. Although there were great fluctuations in MMR of stomachs in cases with duodenal ulcers, it was generally high (average 1.233). The age distribution in this group similated that of the chronic gastritis group. Microscopical examination of the gastric mucosa of the pyloric antrum in chronic gastritis revealed physiological inflammatory changes appearing 20 years earlier than those of aging process. The pattern of microscopical findings in the stomach with small chronic ulcers was similar to that of normal stomach, and the formation of ulcer was interpreted to be a result of an episode occurring locally. In the stomach of cases with duodenal ulcers, other than the low incidence of intestinal metaplasia, the general findings were similar to those seen in chronic gastritis.
LIPOMA OF THE SUPERIOR MEDULLARY VELUM WITH SCHWANN CELLS:Report of a Case and Review of the Literature
Authors: Hara Masamichi , Kawachi Shiro , Hirano Asao
Published: August 2010

A large intracranial lipoma, located on the superior medullary velum Is reported. Histologically, the tumor consisted of adult adipose tissue. The right trochlear nerve was involved within the tumor, and the scattered nests of Schwann cells and some larger vessels were also present. Lipoma of the dorsal region of the mid‐brain is rare and has not been reported from Japan. Schwann cell proliferation in the lipoma is another unusual feature in this case. The histogenesis of this tumor is discussed, and the literature on lipomas of the dorsal region of the mid‐brain is reviewed.
ARTERIOVENOUS MALFORMATION OF THE BRAIN:Histological Study and Micrometric Measurement of Abnormal Vessels
Authors: Isoda Kotaro , Fukuda Haruki , Takamura Nobuo , Hamamoto Yuji
Published: August 2010

An autopsy case of arteriovenous malformation (AVM) of the brain In a 29‐year‐old housewife was reported. Several important and characteristic findings were obtained by detailed histological examination and micrometric measurement of abnormal vessels composing the nidus of the AVM. Structural imperfectness and immaturity of their vascular wall suggested that the AVM is a histoembryogenlc maldevelopment. Prominent dilatation in calibre, hypertrophy of muscular layer, hyallnization, and abnormal increasing of elastic fibers were interpreted as the result of changed cerebral hemodynamics caused by an arteriovenous fistulous communication.
EXPRESSION OF IgD ON B CELL MALIGNANCY|An Immunopathological Study of 50 Cases
Authors: Mori Shigeo , Hagiwara Shizuo , Kodo Hideki , Mohri Noboru
Published: August 2010

Expression of IgD was studied immunopathologically on 50 cases of B cell lymphomas (B MLs), together with various B cell markers including IgM, ϰ and Λ chains, B1, B2, (OK)B2, (OB)B7, (OK)T10, NUB1, Leu 1, Leu 14, and PCA. IgD was demonstrated on 19 cases heavily and on 8 weakly. It associated well with expression of two antigens, B2 (C3d receptor molecule) and Leu1 (pan‐T antigen), besides IgM, while, B2 was closely related in expression on B MLs to (OK)B2, (OK)B7, and NUB1. Λ chain was dominant on IgD heavily‐stained cases. Histopathologically, IgD positive MLs were distributed in various types. All of diffuse intermediate type were shown to be IgD positive (4/4, 3 heavily). As cases of this type were shown to express most of other B cell antigens present on non‐tumorous primary follicle B cells or mantle zone B cells, this type of MLs is speculated to be a neoplastic counterpart of such non‐tumorous B cells. Eight out of 18 cases of diffuse large cell type were IgD heavily positive, suggesting some unusual mechanisms in IgD expression on these neoplastic large cells, as non‐tumorous B cells lose most of their surface IgD soon after blastoid transformation. Other types of MLs were discussed with special emphasis on expression of IgD. ACTA PATHOL. JPN. 36: 1429‐1440, 1986.
BLAST GRISIS OF CHRONIC MYELOGENOUS LEUKEMIA| Morphologic and Immunological Features
Authors: Nemoto Keiichi , Honma Keiichi , Fukuda Takeaki , Ohnishi Yoshihisa
Published: August 2010

The morphological and immunological features of 22 patients with transformation of Philadelphia chromosome (Ph1) positive chronic myelogenous leukemia (CML) were evaluated. Patterns of differentiation in blast crisis were as follows: myeloblastic 5 cases, lymphoblastic 6 cases, basophil differentiation 4 cases, monoblastic 3 cases, megakaryoblastic 1 case, and mixed 3 cases (myeloblastic and lymphoblastic, monoblastic and lymphoblastic and panmyelosis). Three cases were diagnosed as acute lymphoblastic leukemia (ALL) in early stage. After complete remission was achieved rapidly with vincristine and prednisolone, the hematological findings showed CML features. The immunological phenotype of lymphoblasts in lymphoid crisis was Ia+, CALLA+, surface and intracytoplasmic immunoglobulin negative. It was suggested that the neoplastic cells in two cases had B cell differentiation because of B1 or B4 positive. The terminal deoxynucleodidyl transferase (TdT) activity was examined by immunoperoxidase and immunofluorescence techniques on 11 cases. All TdT+ cells were the small lymphoid cells in 5 cases of lymphoid crisis and one case in mixed type. Heterogeneity of the terminal phase of CML was recognized. It was thus suggested that blast crisis of CML occurred in the pluripotent stem cell. ACTA PATHOL. JPN. 36: 1441‐1454, 1986
EMBRYONAL RHABDOMYOSARCOMA IN NUDE MICE AND IN VITRO
Authors: Motoyama Teiichi , Watanabe Hidenobu , Yamamoto Takahiko
Published: August 2010

A transplantable tumor strain desi?nated YNnu and a cultured cell line desi?nated YN were established from the human paratesticular rhabdomyosarcoma of a 15‐year‐old boy. In vitro the cells showed ultrastructural features of immature mesenchymal cells, and a few cells were labeled by antibodies to myo?lobin and/or desmin. In nude mice, the cells became to contain numerous myofibrils with Z‐bands, and considerable number of cells reacted with anti‐myoglobin and/or anti‐desmin antibodies. We conclude that spindle‐shaped or small round cells are the most immature rhabdomyoblast with multiplicity for cellular differentiation. ACTA PATHOL. JPN. 36: 1495‐1505, 1986.
XANTHOGRANULOMA OF THE ORAL CAVITY IN ADULT A Case Report and Review of the Literature
Authors: Takeda Yasunori , Suzuki Atsumi , Fujioka Yukio , Takayama Kazuo
Published: August 2010

A case of xanthogranuloma arising in the palatal gingiva of the maxilla in a 38‐year‐old Japanese male was investigated. Histopathological findings of the excised lesion were indistinguishable from the xanthogranulomas in infants and children (juvenile xanthogranuloma), and like those in the young, the present case was not associated with abnormalities of serum lipids and was not associated with other lesions. A review of the English literature yielded only two histopathologically documented cases of xanthogranuloma in the oral cavity, arising in young patients. A case of xanthogranuloma of oral cavity in an adult was not documented previously. ACTA PATHOL. JPN. 36: 1565‐1570, 1986.
SYSTEMIC AMYLOIDOSIS TERMINATING IN CARDIAC INSUFFICIENCY
Authors: Ishimura Eiji , Shoji Shigeichi , Fujimoto Teruo
Published: August 2010

An autopsy case of systemic amyloidosis is presented. The patient, a 57‐year‐old male, died of congestive heart failure. Autopsy revealed systemic amyloidosis which involved the heart, liver, spleen, kidneys, lymph nodes, tongue, prostate, rectum, and small blood vessels of various organs. In the heart, amyloid deposited diffusely in the myocardium, subendocardial tissue, and blood vessel walls. A lot of vacuolated cardiac muscle fibers were noted. Electron microscopic examination of the myocardium revealed that amyloid fibrils surrounded most of the cardiac muscle fibers in the very close vicinity of their basement membranes. Fashion of the amyloid deposition throughout various organs and the mechanism of heart failure are discussed. ACTA PATHOL. JPN. 36:1593‐1604, 1986.
POLYMYOSITIS ASSOCIATED WITH DISSECTING ANEURYSM OF ARTERIES AND INTRACEREBRAL HEMORRHAGE
Authors: Hatanaka Kaoru , Yutani Chikao , Fujieda Toshiyuki , Yamaguchi Takenori
Published: August 2010

Dissecting aneurysm and stroke are a rare complication of polymyositis. The present report describes an autopsy case of a 53‐year‐old woman, who suffered from polymyositis accompanied by dissecting aneurysms of right external iliac and right renal arteries, and furthermore, intracerebral hemorrhage. The latter was caused by necrotizing angiitis. The patient had no abnormal anatomical changes such as coarctation of aorta, aortic stenosis, bicuspid aortic valve or Marfan's syndrome. Atherosclerosis in the patient was mild, and cystic medial necrosis of aorta and arteries was not found. Since the patient was attacked by dissections of arteries following long‐term steroid therapy, a possibility can be raised that arterial dissection was attributable to steroid treatment besides necrotizing angiitis complicating in polymyositis.
THE HISTOGENESIS and EARLY INVASION OF GASTRIC CANCER
Authors: Saito Kaoru , Shimoda Tadakazu
Published: August 2010

A three‐dimensional analysis of the histogenesis of gastric cancer was made by examining minute, microscopic lesions of less than 500 /z. Cancer develops from the generative cell zone (G‐zone) of the atrophic gastric glands and intestinal metaplastic glands. They then bud or branch and infiltrate into the propria interstitial tissue. The G‐zone of the cancerous glands showed marked expansion with complete loss of normal cell kinetics of gastric glands and with hardly any signs of differentiation into pyloric glands. In the adjacent glands there were also expansion of the G‐zone and formation of loop‐shaped glands with abnormal branching and anastomosis sometimes leading to the formation of a network of glands. These changes are compatible with those of abnormal differentiation accompaning atrophy or intestinal metaplasia and are considered to express a preinvasive cancer. ACTA PATHOL. JPN. 36: 1307–1318, 1986.
SCANNING ELECTRON MICROSCOPIC STUDY OF PRECANCEROUS and CANCEROUS PANCREATIC LESIONS INDUCED BY N‐NITROSOBIS (2‐OXOPROPYL) AMINE IN SYRIAN GOLDEN HAMSTERS
Authors: Naito Zenya , Takahashi Miohihito , Furukawa Fumio , Toyoda Kazuhiro , Hasegawa Ryohei , Hayashi Yuzo
Published: August 2010

Sequential alteration of Syrian golden hamster pancreatic ducts and ductules was investigated by scanning electron microscope during and subsequent to 10 weekly subcutaneous injections of N‐nitrosobis (2‐oxopropyl) amine (BOP). By examining surface morphology of ductal cells and resin casts of the secretory branches arising from the common duct, a series of lesions ranging from slight derangement of the luminal dimensions and loss of cilia to marked projections of “finger‐like” processes accompanied by pleomorphism at the cellular and microvilli levels and increase in numbers of goblet cells could be established. A ductal histogenesis is proposed on the basis of the present scanning electron microscopic and earlier light microscopic findings. ACTA PATHOL. JPN. 36: 1359–1366, 1986.
INDUCTION OF UNDIFFERENTIATED TUMORS BY JC VIRUS IN THE CEREBRUM OF RATS
Authors: Ohsumi Shozo , Motoi Makoto , Ogawa Katsuo
Published: August 2010

Newborn Sprague‐Dawley rats were inoculated intracranially with JC virus (Tokyo‐1), a human polyomavirus, which had been isolated by Nagashimaet al. from the autopsied brain of a patient with progressive multifocal leukoencephalopathy in Japan. Twenty‐one to 70 weeks later, 21 of 27 rats developed brain tumors in the cerebrum, but not in the cerebellum. Most of the tumor cells were of an undifferentiated neuroectodermal nature and showed nuclear palisades and pseudorosettes. In some tumor cells glial flbrillary acidic protein was positive immunohistochemically, and many glial filaments were demonstrated ultrastructurally. Neuronal differentiation was not proved. Two continuous lines of cultured tumor cells were established, and T antigen of JCV (Tokyo‐1) was present in both cell lines. Glial differentiation was confirmed also in the tumors produced by subcutaneous transplantation of cultured tumor cells.
FAMILIAL HEMOPHAGOCYTIC RETICULOSIS Clinicopathologic Findings, and Cytochemical, Immunohistochemical and Electron: Microscopic Studies
Authors: Nemoto Keiichi , Ohnishi Yoshihisa
Published: August 2010

Clinicopathologic findings of a case of familial hemophagocytic reticulosis (FHR), for which splenectomy, and biopsy of the liver and mesenteric lymph nodes were performed, is reported. Histologically, the proliferated cells consisted of not only histiocytes showing severe erythrophagocytosis, but also lymphoid cells of various sizes. The lymphoid cells had atypical features including mitotic figures and convoluted nuclei, and were suspected to be T cells as a result of immunohistochemical studies. We therefore consider that FHR is a special type of lymphoproliferative disease accompanied by severe proliferation of erythrophagocytic histiocytes rather than being a true histiocytic disease. Cytochemical and electron microscopic findings of the involved spleen are also presented.
COMPARATIVE MICROCYTOMETRIC ANALYSIS OF EUROPEAN PERIPHERAL T‐CELL MALIGNANT LYMPHOMAS (EPTL) AND ADULT T‐CELL LEUKEMIA/LYMPHOMAS (ATLL) IN JAPAN
Authors: Hasui Kazuhisa , Sato Eiichi , Hansmann Martin‐Leo , Tokudome Takahiro , Tokunaga Masayoshi
Published: August 2010

A simultaneous microcytometric analysis of nuclear DNA content and size was performed in 8 European peripheral T cell lymphomas (EPTL) and 8 adult T‐cell leukemia/lymphomas (ATLL), comparing their patterns on the nuclear density scattergram (NDS) of DNA content versus nuclear size. The intermingling lymphocytes with or without irregular‐shaped nuclei in EPTL and ATLL were interpreted as stimulated reactive. Medium‐sized cell‐dominated T‐zone lymphomas and ATLL pleomorphic medium‐sized cell type showed two distribution patterns in the diploid range. The first was oblique zonal cluster (OZC) with the second high concentration in the middle part of it, suggesting mixed proliferation with stimulated reactive lymphocytes, and the second was the high concentration in the lower region of the NDS with some cells corresponding to the growth fraction. In large cell‐dominated T‐zone lymphomas and ATLL pleomorphic large cell type, the third decreasing pattern in the cell density from the lower part of the OZC to its upper part was found upto the hypertetraploid range. The wide distribution on DNS and giant cells with aneuploid high DNA content were found only in the pleomorphic large cell type and the pleomorphic medium‐sized and large cell type of ATLL. The T‐immunoblastic type of EPTL showed the third pattern and the pleomorphic large cell type of EPTL, characteristic in the clear cytoplasm, showed the second pattern.
DISTRIBUTION OF IMMUNOGLOBULIN CONTAINING CELLS IN HUMAN BONE MARROW OF PATIENTS WITH LEUKEMIA
Authors: Suzuki Kenshi , Hirokawa Katsuiku , Kamiyama Ryuichi , Hatakeyama Shigeru
Published: August 2010

In the bone marrow of leukemic patients (31 cases), the number of Immunoglobulin containing cells per unit square significantly decreased to the level of approximately 20–50% of the age‐matched control, although the serum level of immunoglobulin in these patients was almost comparable to the control level. The number of immunoglobulin containing cells in the bone marrow was inversely related with the degree of infiltration of leukemic cells and the correlation of Ig‐G containing cell count (Y) and percentage of blast cells (X) was Y = 91.8–1.9X (r =−0.436, P<0.05). Follow‐up examination of the bone marrow after chemotherapy revealed that the recovery of the number of immunoglobulin containing cells in the remission state appeared to occur in younger patients, but not in older ones.
PROTEIN SYNTHESIS IN ITO CELLS (FAT‐STORING CELLS) OF CULTURED LIVER TISSUES FROM CCL4‐TREATED MICE A Light and Electron Microscopic Autoradiographic Study
Authors: Enzan Hideaki
Published: August 2010

Small tissue blocks of livers obtained from normal and CCI4 ‐treated mice were incubated in culture medium supplemented with 3H‐leucine for one hour to observe the protein synthesis of Ito cells in acute carbon tetrachloride liver injury by a light and electron microscopic autoradtography. In normal mouse liver very few Ito cells are labeled with 3H‐leucine. On the contrary, in acutely damaged mouse liver by a single intraperitoneal Injection of CCI4, the Ito cells in the centrilobular zones where liver cells show marked degeneration and necrosis are densely labeled. No labeling, however occurs over the Ito cells in the intermediate and peripheral zones of the same liver lobules, where liver cells showed no cellular degeneration. These findings show that the Ito cells are inactive in 3H‐leucine incorporation under physiological conditions. After acute liver cell injury the Ito cells located in the necrotic areas of liver cells are markedly activitated for protein synthesis.
HIGH ALKALINE PHOSPHATASE ACTIVITY OF TELANGIECTATIC OSTEOSARCOMA (TOS) AND ITS DIAGNOSTIC SIGNIFICANCE
Authors: Yoshida Haruhiko , Adachi Hironobu , Naniwa Shingo , Yumoto Tokichi , Morimoto Kanehito , Furuse Kiyowo
Published: August 2010

Telangiectatic osteosarcoma (TOS) which occurred in the metaphysis of the right femoral bone in a 13‐year‐old female was reported. It showed osteolytic and cystic lesion without sclerotic change on roentgenogram and consisted histologlcally of various sized blood‐filled spaces lined by layers of round to oval tumor cells in the thin fibrous septa. In some solid areas, a proliferation of atypical tumor cells with large prominent nucleoli was evident, embedded in the lace‐like osteoid tissue. Mitotic cells were easily encountered. A large population of tumor cells revealed high alkaline phosphatase activity as well as 5'‐nucleotidase activity, indicative of osteogenic cell origin. Ultrastructurally, they showed osteogenic characteristics of well‐developed rough endoplasmic reticula, cytoplasmic microfibrils, and dense bodies, but not for those of endothelial cells. In this report, we suggest that alkaline phosphatase activity in biopsy and surgical specimens is useful for distinguishing TOS from other osteolytic bone tumors, with regard to its ontogenic discussion.1987.
MERKEL CELL TUMOR COEXPRESSING CYTOKERATIN AND NEUROFILAMENT PROTEINS
Authors: Sadahira Yoshito , Nakamoto Shu , Mori Masaharu , Hsueh Chao Liang , Awai Michiyasu
Published: August 2010

Whorled filaments 10 nm in width were identified by anti‐intermediate filaments antibodies in a Merkel cell tumor from a 52‐year‐old man. Immunohistochemlcal tests revealed that the tumor was stained with anti‐keratin antibody and antibodies against the 68‐kd and 200‐kd subunits of neurofilament proteins but not antibody against the 150‐kd subunlt. This is the first reported case of Merkel cell tumor expressing a 200‐kd subunit of neurofilament proteins.
Effect of Anti‐androgen (TZP‐4238) on Steroid‐induced Canine Prostatic Hyperplasia
Authors: Murakoshi Masanori , Inada Rie , Makino Masao , Suzuki Minoru , Mieda Mamoru , Honma Seiziroh , Yamanaka Hidetoshi
Published: August 2010

The effect of a synthetic steroidal anti‐androgen, TZP‐4238, on steroid‐induced canine prostatic hyperplasia was studied by light and electron microscopy. Male beagle dogs (1–2 years old) were divided into four experimental groups. Group 1 consisted of intact controls. The other animals were castrated. The castrated animals were treated for 25 weeks with 1) 5α‐androstane‐3α, 17β‐diol (A‐diol) plus 17β‐estradiol (E2)(Group2), 2) A‐diol plus E2+ TZP 4238 0.5mg/kg (Group 3) and 3) A‐diol plus E2+ chlormadinone acetate (CMA) 2.5 mg/kg (Group 4). TZP‐4238 and CMA were administered orally for 21 weeks after 4 weeks treatment with A‐diol plus E., In group 2, glandular hyperplasia of the prostate was clearly noted. In contrast, combined treatment with TZP‐4238 (Group 3) or CMA (Group 4) produced marked atrophy of the glandular epithelium. Loss of secretory and metabolic activities was confirmed by ultrastructural investigations. Our data indicate that TZP‐4238 is a potent anti‐androgen for the prevention of canine prostatic hyperplasia in the steroid‐induced benign prostatic hyperplasia (BPH) model. Acta Pathol Jpn 40: 871–879, 1990.
Epithelial Cell Nodules Developing in the Cecum of Irradiated Mice
Authors: Fukushima Yukio , Imada Masato , Nagoshi Junsuke , Shiozaki Hitoshi , Mori Takesada , Fujita Hisao , Kitamura Yukihiko
Published: August 2010

A method for producing macroscopic epithelial nodules was developed in order to investigate the properties of epithelial stem cells in the large intestine of mice. The cecum was exteriorized and exposed to various doses of X‐ray radiation. Numbers of nodules subsequently developing were counted and plotted against radiation dose. From the logarithmic regression line, the susceptibility to irradiation of nodule forming stem cells (NFSC) was determined. The susceptibility of cecal NFSC was comparable to the value reported for jejunal NFSC. Mice of (C57BL/6XDS) F1‐Pgk‐1b/Pgk‐1a that carried X chromosome inactivation mosaicism for the phosphoglycerate kinase gene were used for examination of nodule clonality. Most cecal nodules contained only 1 type of phosphoglycerate kinase, suggesting a monoclonal origin of the nodules. Histochemical studies showed the presence of absorptive epithelial, goblet and entero endocrine cells in 17 day old nodules, implying multipotentiality of the NFSC. In spite of these similarities between cecal and jejunal NFSC, the macroscopic appearance of cecal nodules was quite different from that of jejunal nodules. Only crypt like structures were observed in the former, whereas both crypt like and villus like structures were present in the latter. A comparison between cecal and jejunal nodules may be useful for understanding the morphogenesis of the intestinal mucosa. Acta Pathol Jpn 40: 880–886, 1990.
Hepatocellular Carcinoma Presenting Well Differentiated, Normotrabecular Patterns in Peripheral or Metastatic Loci
Authors: Komatsu Teisuke , Kondo Yoichiro , Yamamoto Yoshikazu , Isono Kaichi
Published: August 2010

In a series of 103 surgically resected hepatocellular carcinomas (HCCs), the tumor boundaries were rather clearly defined in 85 cases (82.5%). Twelve tumors had an ill‐defined margin because adjacent pseudolobules were replaced by HCC with a well differentiated growth pattern (group Al). This peripheral pattern was unrelated to the predominant histologic type of the major tumor nodules. Despite its close resemblance to normal hepatic parenchyma, we were able to determine the area of the normotrabecular HCC on the basis of, differences in stain‐ability, variable nuclear crowding and frequent microacinar formation. Moreover, scattered occurrence of nuclear atypia and midtrabecular conversion were also observed within the normotrabecular HCC. In an additional 6 cases, a band like zone of normotrabecular HCC was evident peripherally around the primary lesion (group A2). Fourteen other cases (13.6%) had intrahepatic metastatic lesions formed either within the portal vein or in pseudolobules. Of these, 5 cases showed intrapseudo‐lobular metastases with a normotrabecular pattern (group B). For precise evaluation of surgical HCC specimens, it seems to be particularly important to realize that HCCs sometimes have a highly differentiated appearance in a locus of peripheral invasion as well as intrahepatic metastasis. Acta Pathol Jpn 40: 887–893, 1990.
Immunohistochemical Demonstration of Cell Proliferation and Estrogen Receptor Status in Human Breast Cancer
Authors: Hori Masao , Furusato Masakuni , Nikaidoh Takashi , Aizawa Shigeo
Published: August 2010

Cell proliferation and estrogen receptor (ER) status was investigated in 45 invasive ductal carcinomas of the breast by immunohistochemical methods using monoclonal antibodies Ki 67 (anti‐human proliferating cell antibody) and ER ICA. The results were assessed on the basis of nuclear staining intensity and the percentage of positively stained tumor cell nuclei (index score). There was a significant inverse correlation between the Ki 67 and ERICA index scores, although 4 cases showed high index scores for both markers. We conclude that ER positive cells do not always have low proliferation activity, which may be one of the reasons why endocrine therapy is not effective against all ER positive breast cancers. Acta Pathol Jpn 40: 902 907, 1990.
Microlamellar Structures in Lobular Glomerulonephritis Associated with Monoclonal IgG Lambda Paraproteinemia
Authors: Joh Kensuke , Aizawa Higeo , Takahashi Takamune , Hatakeyama Makio , Muto Shigeaki , Asano Yasushi , Shimizu Hideo , Suzuki Ryoji
Published: August 2010

We report a 49 year old woman who developed lobular glomerulonephritis with prevalent deposition of material positive for IgG, C1q and lambda light chain, but which was not stained by Congo red. Glomeruli revealed massive electron‐dense deposits with a microlamellar structure in the mesangial matrix and peripheral capillary loops. Clinically, the patient had nephrotic syndrome, microhematuria and hypertension. No Bence Jones protein or cryoglobulin was found in the urine or serum. Anti‐DNA antibody was positive, but systemic lupus erythematosus (SLE) was ruled out by repeated serological examinations. Immunoelectrophoresis of blood and urine revealed increased IgG lambda paraprotein, but no free light chains were found. We reviewed 54 cases reported in the literature, which showed organized crystalline structures on ultrastructural examination, but were unassociated with amyloidosis, SLE, cryoglobulinemia or multiple myeloma. The present patient is the first reported to have exhibited a combination of glomerulonephritis with organized deposits, monoclonal IgG lambda paraproteinemia, and the presence of anti DNA antibody. Acta Pathol Jpn 40: 913 921, 1990.
Clinicopathologic and Immunohistochemical Characteristics of Goblet Cell Type Adenocarcinoma of the Lung
Authors: Gemma Akihiko , Noguchi Masayuki , Hirohashi Setsuo , Tsugane Shoichiro , Tsuchiya Ryosuke , Niitani Hisanobu , Shimosato Yukio
Published: August 2010

Twenty‐two resected goblet cell type adenocarcinomas of the lung were examined clinicopathologically and immunohistochemically. The stage and survival curve of goblet cell type adenocarcinomas were compared with those of 44 cases of pure or mixed Clara cell and bronchial surface epithelial cell (Clara and BSE) type adenocarcinomas. Each case of goblet cell type was matched with two cases of Clara and BSE type as to sex, age and date of surgery. In goblet cell type adenocarcinomas, lymph node metastasis was less frequently and intrapulmonary metastasis was more frequently detected than in other types of adenocarcinomas (p < 0.001 and p < 0.05, respectively). Goblet cell type adenocarcinomas showed better prognoses than Clara and BSE type adenocarcinomas. However, the estimated survival curves of those two groups become similar after adjustment of the TNM condition using Cox's proportional‐hazard general linear model. This result indicated that the longer survival of goblet cell type adenocarcinoma was due to the characteristic distribution of TNM conditions, that is, unique local growth and low incidence of lymph node metastasis. When goblet cell type adenocarcinoma was macroscopically classified into two types, i.e. solitary peripheral nodule type (nodular type) and multifocal nodular type or consolidation of all or part of a lobe (diffuse type), the nodular type had better prognosis than the diffuse type (p < 0.05). Immunohistochemically, 83%, 11%, and 0% of goblet cell type adenocarcinomas were positive for NCC‐CO‐450, carcinoembryonic antigen (CEA), and surfactant apoprotein, respectively. Most Clara and BSE type adenocarcinomas were negative for NCC‐CO‐450, but positive for CEA and surfactant apoprotein. NCC‐CO‐450 was considered to be a good immunohistochemical marker of goblet cell type adenocarcinoma of the lung. These results indicated that goblet cell type tumors are different from most adenocarcinomas of other types both clinicopathologically and immunohistochemically. Acta Pathol Jpn 41: 737‐743, 1991.
Alveolar Soft Part Sarcoma of the Pulmonary Vein
Authors: Tsutsumi Yutaka , Deng Yinlong
Published: August 2010

Alveolar soft part sarcoma of the lung seen in a 42‐year‐old female is reported. In the partial pneumonectomy specimen, there was a 3x2.5 cm tumor arising from the pulmonary vein at the level of the right lung hilus, with tumor thrombus formation. The transition between the tumor and venous smooth muscle layer was microscopically confirmed. At autopsy, performed 18 months after surgery, metastases were noted in the left lung and brain. No primary focus was identified in the soft tissue. The alveolus‐forming clear tumor cells contained diastase‐resistant periodic acid‐Schiff‐reactive granules. Immunohistochemically, granular cytoplasmic reactivities with monoclonal antibodies against pan‐actin and alpha‐sar‐comeric actin were demonstrated, whereas other muscle markers such as desmin, alpha‐smooth muscle actin, myoglobin, fast skeletal myosin, and the mm‐isozyme of creatine kinase were negative. Ultrastructurally, crystallized structures were occasionally identified in the membrane‐bound, electron lucent granules, which often filled the tumor cell cytoplasm. The muscle cell nature of the neoplasm is discussed. Acta Pathol Jpn 41: 771‐777, 1991.
A Light and Electron Microscopical Study of Membranocystic Lesions in a Case of Lupus Erythematosus Profundus
Authors: Kuwabara Hiroko , Uda Hirotsugu , Saito Katsuhiro
Published: August 2010

A light and electron microscopical study on membranocys‐tic lesions (MCL) in a case of lupus erythematosus profundus (LEP) is reported. The patient was a 16 year old female who presented with subcutaneous nodules on both upper arms. The light microscopic features were consistent with LEP, and the result of an immunofluorescence band test supported this diagnosis. A peculiar finding in this case was MCL in the subcutaneous tissue. Ultra‐structurally, these were thin membranes without a tubular structure and tortuous thick membranes composed of minute tubules. The lesions were very similar to the fatty tissue changes in membranous lipodystrophy. On the other hand, the basement membranes of the blood vessels were thickened and multilayered, and the Iumina were narrowed by endothelial swelling and thickening of the vessel wall. Our findings suggest that the MCL in LEP result from circulatory disturbance of the fat tissue.
Ovarian Mucinous Cystadenocarcinoma Producing aIpha‐Fetoprotein:A Case Report
Authors: Nomura Kouichi , Miyasaka Yuri , Murae Masamoto , Terashima Yoshiteru , Aizawa Shigeo
Published: August 2010

A 62‐ year‐ old woman with a complaint of abdominal dis‐tention underwent surgery to remove a tumor in her left ovary. The serum alpha‐ fetoprotein (AFP) level was 4,130 ng/ml. Histologically, the tumor was diagnosed as a mucinous cystadenocarcinoma with a small area of solid proliferation of tumor cells. The cells of the latter part were shown immunohistochemically to possess AFP. We suppose that a part of the mucinous cystadenocarcinoma dedifferentiates and produces AFP. Acta Pathol Jpn 42: 372–375. 1992.
Rhabdomyosarcoma with FocaI Cartilaginous Differentiation (Malignant Mesenchymoma) of the Inferior Vena Cava
Authors: Kuwashima Yoshio , Hayashi Shota , Arata Makoto , Takemura Tamiko
Published: August 2010

A sarcoma arising from the inferior vena cava occupied the entire lumen of the inferior vena cava, right atrium, hepatic veins and conmon iliac veins. Its histological appearance was non‐ specific sarcoma, except for the presence of a few rhabdomyoblasts and some immature cartilaginous tissue. lmmunohistochemically, some tumor cells were positive for myoglobin, desmin, HHF‐35, and vimentin. Electron microscopy revealed that some tumor cells contained myofilaments and Z bands in the cytoplasm, which are characteristics of rhabdomyosarcoma. The tumor was diagnosed as rhabdomyosarcoma with focal cartilaginous differentiation (malignant mesenchymoma) of the inferior vena cava. Acta Pathol Jpn 42: 382–385, 1992.
Benign Follicular Thyroid Nodule Composed of Signet‐ring‐like Cells with PAS‐negative Thyroglobulin Accumulation in Dilatated Rough Endoplasmic Reticulums
Authors: Mochizuki Makoto , Saito Ken , Kanazawa Kyotaro
Published: August 2010

A benign follicular thyroid nodule composed of signet‐ringlike cells in a 46 year old Japanese euthyroid woman is reported. Thyroglobulin was positive, and periodic acid‐Schiff (PAS) and other mucin stainings were negative in the cytoplasm of signet‐ring‐like cells. Ultrastructually their cytoplasm contained many dilatated rough endoplasmic reticulums (rERs), the largest of which compressed the nucleus toward the cellular apical portion. Functional disturbance of rER or Golgi apparatus might be the cause of this characteristic cellular change.
Epidermal Inclusions in Abdominal Lymph Nodes
Authors: Arai Tomio , Kino Isamu , Nakamura Shinichi , Ogawa Hiroshi
Published: August 2010

Abdominal lymph nodes from men aged 48 and 62 years with gastric adenocarcinoma were examined histologically. A few of the lymph nodes contained epidermal epithelium, although there was no metastasis. Scattered squamous epithelial nests and epidermal cysts were found within the lymph nodes, and small amounts of pancreatic tissue were present adjacent to the epidermal inclusions. Some squamous cell nests showed cyst formation, and some others showed squamous cell nests together with mucin containing cells forming luminal structures. Immunohistochemically, the squamous cell nests stained weakly for CA19–9 but were negative for CEA or CA125. These lesions were considered to be similar to lymphoepithelial cysts of the pancreas.
Malignant Myoepithelioma of Minor Salivary Gland Origin
Authors: Takeda Yasunori
Published: August 2010

Myoepithelial cells are a significant component of most types of salivary gland tumors. A small but increasing number of case reports have also shown that true myoepithelioma (ME) forms a distinct clinicopathologic entity of salivary gland tumors with unique histologic features, however, the malignant type of ME is exceedingly rare. The present paper reports a case of malignant ME originating from the palatal minor salivary gland. The patient was a 70 year old Japanese male with recurrent tumor of the palatal region. Pathologically, the tumor consisted of proliferating polygonal shaped cells and plump spindle cells with cellular atypia and frequent mitoses, forming lumen less lobuli or strands. Clear tumor cells were also found in part. The stroma was poorly developed without any myxoid or chondroid features. Immunohistochemical study showed positive stainings for S 100 protein, actin and vimentin in the tumor cells. Ultrastructurally, tumor cells had features of myoepithelial cells.
Signet Ring Cells in Breast Carcinoma
Authors: Yoshida Aichi , Hatanaka Shingo , Oneda Satoru , Yoshida Hiroki
Published: August 2010

Two cases of breast carcinoma composed predominantly of neoplastic cells with a signet ring appearance, one a case of invasive lobular carcinoma (ILC) and the other a case of invasive ductal carcinoma (IDC), were examined electron microscopically and immunohistochemically. In signet ring cells in the ILC, mucin was demonstrated ultrastructur‐ally in the intracytoplasmic lumina and also to a small degree in the cytoplasmic mucous granules, whereas in signet ring cells in the IDC, mucin was found only in the cytoplasmic mucous granules. Immunohistochemically, signet ring cells in the ILC were intensely positive for gross cystic disease fluid protein (GCDFP‐15), but those in the IDC showed no immunoreaction for GCDFP‐15. Thus ultrastructural features and GCDFP 15 immunoreactivity appear to be useful for distinguishing between the two different types of signet ring cells.