bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a Model for Performance Analysis of a Honeycomb Thermal Energy Storage for Solar Power Microturbine Applications
Authors: Iaria Davide , Zhou Xin , Al Zaili Jafar , Zhang Qiang , Xiao Gang , Sayma Abdulnaser
Published: October 2019

Solar power microturbines are required to produce steady power despite the fluctuating solar radiation, with concerns on the dispatchability of such plants where thermal energy storage may offer a solution to address the issue. This paper presents a mathematical model for performance prediction of a honeycomb sensible-heat thermal energy storage designed for application of concentrated solar power microturbine. The focus in the model is to consider the laminar developing boundary layers at the entry of the flow channels, which could have a profound effect on the heat-transfer coefficient due to large velocity and temperature gradients, an effect which has not been considered in the modelling of such storage systems. Analysing the thermal and hydrodynamic boundary layer development, the Nusselt number and the friction factor were evaluated using a validated conjugate heat-transfer method. The simulations results were used to develop accurate regression functions for Nusselt number and friction factor. These formulations have been adopted within a one-dimensional model to evaluate the performance of the storage under different operating conditions. The model was in good agreement with conjugate heat transfer results with maximum relative error below 2%. Two case studies are presented to demonstrate the applicability of the proposed methodology.
Methanol Marker for the Detection of Insulating Paper Degradation in Transformer Insulating Oil
Authors: Jalbert Jocelyn , Rodriguez-Celis Esperanza M. , Arroyo-Fernández Oscar H. , Duchesne Steve , Morin Brigitte
Published: October 2019

This manuscript presents a comprehensive literature review with the aim to provide readers a reference document with up-to-date information on the field of methanol use as a chemical marker. It has been a little more than a decade since methanol was first introduced as a marker for assessing solid insulation condition in power transformers. It all started when methanol was identified in the laboratory during thermal ageing tests carried out with oil-immersed insulating papers and was subsequently also identified in transformer field samples. The first publication on the subject was released in 2007 by our research group. This review covers the methanol fundamentals such as the analytical methods for its determination in transformer oil, which is generally performed by headspace gas chromatography with mass spectrometry or flame ionization as a detector. Current standardization efforts for its determination include ASTM working group 30948 and IEC TC10. Kinetic studies have confirmed the relationship between methanol generation, the number of broken 1,4-β-glycosidic bonds of cellulose and changes in mechanical properties. Laboratory tests have confirmed its stability at different accelerated ageing temperatures. Several utilities have identified methanol during field measurements, case studies on power and some distribution transformers are presented, as well as transformer postmortem investigations. These field-testing results demonstrate its utility in monitoring cellulosic insulation degradation. Recently, a model of methanol interpretation has become available that allows for evaluation of the average degree of polymerization of core type transformer cellulose winding. Methanol has a role as an indicator of cellulosic solid insulation ageing in transformer mineral oil, and it is expected that in the future it will be in routine use by utilities.
Autotoxicity Hinders the Natural Regeneration of Cinnamomum migao H. W. Li in Southwest China
Authors: Huang Xiaolong , Chen Jingzhong , Liu Jiming , Li Jia , Wu Mengyao , Tong Bingli
Published: October 2019

Autotoxicity is a widespread phenomenon in nature and is considered to be the main factor affecting new natural recruitment of plant populations, which was proven in many natural populations. Cinnamomum migao H. W. Li is an endemic medicinal woody plant species mainly distributed in Southwestern China and is defined as an endangered species by the Red Paper of Endangered Plants in China. The lack of seedlings is considered a key reason for population degeneration; however, no studies were conducted to explain its causes. C. migao contains substances with high allelopathic potential, such as terpenoids, phenolics, and flavonoids, and has strong allelopathic effects on other species. Therefore, we speculate that one of the reasons for C. migao seedling scarcity in the wild is that it exhibits autotoxic allelopathy. In this study, which was performed from the perspective of autotoxicity, we collected leaves, pericarp, seeds, and branches of the same population; we simulated the effects of decomposition and release of litter from these different anatomical parts of C. migao in the field; and we conducted 210-day control experiments on seedling growth, with different concentration gradients, using associated aqueous extracts. The results showed that the leaf aqueous extract (leafAE) significantly inhibited growth indicators and increased damage of the lipid structure of the cell membrane of seedlings, suggesting that autotoxicity from C. migao is a factor restraining seedling growth. The results of the analyses of soil properties showed that, compared with the other treatments, leafAE treatment inhibited soil enzyme activity and also had an impact on soil fungi. Although leafAE could promote soil fertility to some extent, it did not change the effect of autotoxic substances on seedling growth. We conclude that autotoxicity is the main obstacle inhibiting seedling growth and the factor restraining the natural regeneration of C. migao.
Editorial for Special Issue “Multiscale Turbulent Transport”
Authors: Martins Afonso Marco , Gama Sílvio M. A.
Published: October 2019

Turbulent transport is currently a great subject of ongoing investigation at the interface of methodologies running from theory to numerical simulations and experiments, and covering several spatio-temporal scales [...]
Physicochemical, Sensorial and Microbiological Characterization of PoroCheese, an Artisanal Mexican Cheese Made from Raw Milk
Authors: González Ariceaga Citlalli Celeste , Afzal Muhammad Inam , Umer Muhammad , Abbas Shabbar , Ahmad Haroon , Sajjad Muhammad , Parvaiz Fahed , Imdad Kaleem , Imran Muhammad , Maan Abid Aslam , Khan Muhammad Kashif Iqbal , Ullah Azmat , Hernández-Montes Arturo , Aguirre-Mandujano Eleazar , Villegas de Gante Abraham , Jacquot Muriel , Cailliez-Grimal Catherine
Published: October 2019

Poro cheese is a regional product originally from the area of Los Rios, Tabasco in Mexico. In the context of preserving the heritage of Poro cheese and protecting the specific characteristics that define its typicity through an origin designation, the present study was conducted to establish a general profile of Poro cheese by characterizing their physicochemical, textural, rheological, sensorial and microbiological characteristics. Differences in moisture, proteins, fats, NaCl, titrable acidity, pH, color texture and rheology amongst cheese factories were observed and ranges were established. Fifteen descriptors were generated to provide a descriptive analysis, eight of which were significantly different amongst the factories with no differences in the global acceptability of cheese. The favorite cheese had the highest scores for aroma attributes. Conventional and molecular methods were used to identify the main microorganisms, for which Lactobacillus plantarum, L. fermentum, L. farciminis and L. rhamnosus were the main microorganisms found in Porocheese. The obtained data constituted the parameters for characterizing Poro cheese, which will strongly help to support its origin appellation request process.
“Cheese: Technology, Compositional, Physical and Biofunctional Properties:” A Special Issue
Authors: Moatsou Golfo
Published: October 2019

The objective of the present editorial to critical synopsize articles that make up the Special Issue “Cheese: Technology, Compositional, Physical and Biofunctional Properties.” The published research papers are multidisciplinary studies which refer to some of the most important sub-topics of Cheese Science and Technology. They present the results of experimental studies and analyses that can be further exploited by academia and cheese producers.
Narrow-Leafed Lupin Main Allergen β-Conglutin (Lup an 1) Detection and Quantification Assessment in Natural and Processed Foods
Authors: Lima-Cabello Elena , Alché Juan D. , Jimenez-Lopez Jose C.
Published: October 2019

The increasing prevalence of lupin allergy as a consequence to the functional characteristics of a growing number of sweet lupin-derived foods consumption makes the imperious necessity to develop analytical tools for the detection of allergen proteins in foodstuffs. The current study developed a new highly specific, sensitive and accurate ELISA method to detect, identify and quantify the lupin main allergen β-conglutin (Lup an 1) protein in natural and processed food. The implementation of accurate standards made with recombinant conglutin β1, and an anti-Lup an 1 antibody made from a synthetic peptide commonly shared among β-conglutin isoforms from sweet lupin species was able to detect up to 8.1250 ± 0.1701 ng (0.0406 ± 0.0009 ppm) of Lup an 1. This identified even lupin traces present in food samples which might elicit allergic reactions in sensitized consumers, such as β-conglutin proteins detection and quantification in processed (roasted, fermented, boiled, cooked, pickled, toasted, pasteurized) food, while avoiding cross-reactivity (false positive) with other legumes as peanut, chickpea, lentils, faba bean, and cereals. This study demonstrated that this new ELISA method constitutes a highly sensitive and reliable molecular tool able to detect, identify and quantify Lup an 1. This contributes to a more efficient management of allergens by the food industry, the regulatory agencies and clinicians, thus helping to keep the health safety of the consumers.
Validation of a HILIC UHPLC-MS/MS Method for Amino Acid Profiling in Triticum Species Wheat Flours
Authors: Tsochatzis Emmanouil , Papageorgiou Maria , Kalogiannis Stavros
Published: October 2019

Amino acids are essential nutritional components as they occur in foods either in free form or as protein constituents. An ultra-high-performance (UHPLC) hydrophilic liquid chromatography (HILIC)-tandem Mass Spectrometry (MS) method has been developed and validated for the quantification of 17 amino acids (AA) in wheat flour samples after acid hydrolysis with 6 M HCl in the presence of 4% (v/v) thioglycolic acid as a reducing agent. The developed method proved to be a fast and reliable tool for acquiring information on the AA profile of cereal flours. The method has been applied and tested in 10 flour samples of spelt, emmer, and common wheat flours of organic or conventional cultivation and with different extraction rates (70%, 90%, and 100%). All the aforementioned allowed us to study and evaluate the variation of the AA profile among the studied flours, in relation to other quality characteristics, such as protein content, wet gluten, and gluten index. Significant differences were observed in the AA profiles of the studied flours. Moreover, AA profiles exhibited significant interactions with quality characteristics that proved to be affected based mainly on the type of grain. A statistical and multivariate analysis of the AA profiles and quality characteristics has been performed, as to identify potential interactions between protein content, amino acids, and quality characteristics.
Effect of Communication Regarding Dam Operation on the Evacuation of Residents: A Case Study of the 2018 Inundation of the Hijikawa River in Japan
Authors: Nakamura Isao , Morioka Chiho
Published: October 2019

Communication on the operation of a dam is crucial in evacuating residents before downstream flooding occurs. This paper examines the effect of communication regarding dam operation on evacuation, studying the case of the 2018 flooding of the Hijikawa River in Japan. After confirming the communication process and the messages of warning, we conducted a questionnaire survey of affected residents. The findings of the survey are as follows. (1) The discharge warnings issued by dam operators had no effect, because few people heard the warnings and even those who heard them were not inclined to evacuate. (2) Accepting the notifications from dam operators, local governments issued evacuation instructions. These instructions promoted evacuation. The most effective trigger of evacuation was route alerting by the volunteer fire corps. Information from dam operators induced the issuing of evacuation instructions, which activated the route alerting, and the information therefore indirectly promoted evacuation. (3) The Public Warning System operating on mobile phones had a certain effect in disseminating evacuation instructions where the system was used. (4) The messages issued here had insufficient specificity and clarity. A flood simulation considering the discharge flow of a dam needs to be conducted in addressing this issue.
Quantification of Modelling Uncertainties in Bridge Scour Risk Assessment under Multiple Flood Events
Authors: Pizarro Alonso , Tubaldi Enrico
Published: October 2019

Local scour is a dynamic process evolving during the lifetime of bridges as a result of the changes in hydrologic and hydraulic conditions. Current approaches for scour risk assessment are generally based on the evaluation of the equilibrium scour depth for a flood event with a prefixed return period. The temporal evolution of the bridge-pier scour process is usually disregarded, by assuming that equilibrium conditions are always attained, regardless of the flood properties. However, recent studies have highlighted the importance of accounting for the contribution of multiple flood events and their exact hydrograph shape. This study aims at quantifying the epistemic uncertainty related to the modelling of the temporal evolution of scour under multiple consecutive flood events in clear-water conditions. A simple numerical case study is considered, using a Markovian framework to describe probabilistically the progression of scour. Well-known time-dependent scour models are used to estimate the temporal evolution of the scour-depth under each flood hydrograph, and the scour estimates are compared with those obtained using widely employed equilibrium scour formulas. Results show that the expected scour depth is influenced by the parameters used to describe the flood hydrograph and that the probability distribution of the scour depth is highly sensitive to the choice of the time-dependent scour model. The uncertainty in the scour estimation stemming from the formula adopted in this study for describing the temporal evolution of the scour depth can be higher than those related to the formula adopted for equilibrium scour.
Deformation Modeling of Flexible Pavement in Expansive Subgrade in Texas
Authors: Ahmed Asif , Hossain MD Sahadat , Pandey Pratibha , Sapkota Anuja , Thian Boon
Published: October 2019

The tendency of expansive subgrade soil to undergo swelling and shrinkage with the change in moisture has a significant impact on the performance of the pavement. The repeated cycles of wet and dry periods throughout a year lead to considerable stress concentration in the pavement subgrade soil. Such stress concentrations leads to the formation of severe pavement cracks. The objective of the research is to develop a prediction model to estimate the deformation of pavement over expansive subgrade. Two pavement sites—one farm to market road and one state highway—were monitored regularly using moisture and temperature sensors along with rain gauges. Additionally, geophysical testing was performed to obtain a continuous profile of the subgrade soil over time. Topographical surveying and horizontal inclinometer readings were taken to determine pavement deformation. The field monitoring data resulted in a maximum movement up to 80 mm in the farm to market road, and almost 38 mm in the state highway. The field data were statistically evaluated to develop a deformation prediction model. The validation of the model indicated that only a fraction of the deformation was reflected by seasonal variation, while inclusion of rainfall events in the equation significantly improved the model. Furthermore, the prediction model also incorporated the effects of change in temperature and resistivity values. The generated model could find its application in predicting pavement deformation with respect to rainfall at any time of the year.
Satisfaction Level of Tuberculosis Patients Regarding Their Access to TB Care and Prevention Services, Delivered Through a Public–Private Mix Model in Pakistan
Authors: Ali Syed Mustafa , Anjum Naveed , Naureen Farah , Rashid Aamna , Tahir Adeel , Ishaq Muhammad , Usman Muhammad
Published: October 2019

Objective: The private healthcare providers (PHCP) are believed to improve access to healthcare services in public–private mix (PPM) projects, as they are considered first point of contact for healthcare. The purpose of this study was to determine the satisfaction level of tuberculosis (TB) patients. Design: A questionnaire-based, cross-sectional study was carried out during November and December 2017 for 572 under-treatment patients registered with PHCPs in the PPM project. Lot quality assurance sampling technique was used to randomly select 19 districts from a sample frame of 75 districts. In each selected district, the data collector retrieved a TB register of 8 months (January–August 2017) and systematically selected patients by fixed periodic interval. SPSS version 24.0 (IBM Corp, Amonk, NY, USA) was used to analyze the data. Results: This study included 53% (n = 301) males and 47% (n = 271) females, with mean age of 38 years (SD, ±18). Almost half of the participants were illiterate (51%, n = 289), and 64% (n = 365) were non-earning members of the family. In practice, most of the participants visit private providers (71%, n = 407), including private hospitals/clinics (44%) and traditional practitioners (27%; n = 153); 55% of participants visited their current doctor because of the clinic’s proximity to their residence. Of the participants, 82% (n = 469) were satisfied with TB care services and 85% (n = 488) said that they would recommend this clinic to others. Conclusion: PHCPs are preferred providers for individuals, which is consistent with findings of other studies. Though they are satisfied with TB care and services, interventions should be introduced to reduce the financial burden on the patient. Partnering PHCP is a way forward to ensure universal health coverage and better health outcomes of the population.
Socio-Economic and Environmental Factors Related to Spatial Differences in Human Non-Tuberculous Mycobacterial Diseases in the Czech Republic
Authors: Modrá Helena , Ulmann Vít , Caha Jan , Hübelová Dana , Konečný Ondřej , Svobodová Jana , Weston Ross Tim , Pavlík Ivo
Published: October 2019

Non-tuberculous mycobacteria (NTM) are ubiquitous environmental bacteria that can induce pulmonary and non-pulmonary diseases in susceptible persons. It is reported that the prevalence of NTM diseases is increasing in developed countries, but this differs by regions and countries. NTM species distribution and the rate of diseases caused by NTM vary widely in the historical territories of Moravia and Silesia (Czech Republic). This epidemiologic study of NTM diseases covers the period 2012–2018, reviews isolates obtained from patients with clinical disease and investigates correlations with related socio-economic and environmental factors. Individual NTM patients were included only once during the studied period and results were presented as incidence rate per year. The most frequently isolated NTM meeting the microbiological and clinical criteria in the study were the Mycobacterium avium-intracellulare complex, followed by Mycobacterium kansasii and Mycobacterium xenopi. A previously described endemic incidence of M. kansasii in the Karviná district and M. xenopi in the Ostrava district was also observed in this study. The incidence of NTM patients in the whole studied territory was 1.10/100,000 inhabitants (1.33/100,000 in men and 0.88/100,000 in women). The annual incidence of lymphadenitis in children (≤5 years of age) was 2.35/100,000 of the population of children during the 7 year period but increased in the year 2018 to 5.95/100,000. The rate of human tuberculosis in the studied area was 1.97/100,000 inhabitants. The incidence of NTM pulmonary diseases correlated with a lower socio-economic status (r = 0.63) and a higher concentration of benzo[a]pyrene pollution in the air (r = 0.64).
Suicidality in Women with Adjustment Disorder and Depressive Episodes Attending an Irish Perinatal Mental Health Service
Authors: Doherty Anne M , Crudden Genevieve , Jabbar Faraz , Sheehan John D , Casey Patricia
Published: October 2019

Depression is common in the perinatal period, with prevalence rates of 14.4%, but prevalence rates of adjustment disorder in this period have not been established. We aimed to examine the characteristics of women attending a perinatal psychiatry service diagnosed with adjustment disorder (AD) or depressive episodes (DE). The data were collected as part of a multicentre case-control study of 370 patients, 45 of whom were recruited from perinatal psychiatry service at a maternity hospital. We recruited 45 patients with AD or DE diagnosed in the perinatal period and compared them to a matched sample of 109 non-perinatal women. Almost half, 22 (48.9%) perinatal women had a diagnosis of AD and 23 (51.1%) had a diagnosis of DE. Of the perinatal participants, those with AD had more stressful life events, and suicidal ideation and behaviours were three times more common (31.8%) in AD than in DE (8.7%). There were no significant differences in levels of suicidality between the perinatal and the non-perinatal groups. In our cohort, AD is associated with symptoms of depression including suicidal ideation during the perinatal period. Further study is required to examine the relationship between stressors and suicidality in this population.
Factors Associated with General Health Screening Participation among Married Immigrant Women in Korea
Authors: Jeong Jinhee , Lee Yunhee , Kwon Sung Hee , Myong Jun-Pyo
Published: October 2019

Background: The number of married female immigrants living in Korea has been increasing and is expected to increase further. This study was performed to identify factors associated with national general health screening participation among married immigrant women living in South Korea. Methods: The Korean National Health Insurance System’s (NHIS) customized database for the years 2014 and 2015 was used. The targets of this study were women aged 19 years old and above. To identify factors associated with national general health screening participation, the following analyses were employed: frequency, chi-square, simple regression, and multiple regression. Results: A total of 11,213 women were identified in the NHIS database. Overall, 67.4% participated in national general health screenings, lower than the 74.6% participation rate of the entire women’s health screening program. Married immigrant women with a job had higher health screening participation than those without a job (OR = 2.822, p < 0.0001). Age, socioeconomic status, and duration of stay were related to health screening behaviors among employed married immigrant women. Nationality, socioeconomic status, duration of stay, and disease status were associated with general health screening behaviors among unemployed immigrant women. The odds ratios decreased as the length of stay increased, regardless of employment status. Conclusion: The results of this study showed that employment status and duration of stay in Korea are significantly associated with general health screening participation. Accordingly, to improve awareness about health screening and health care disparities, programs promoting health screening participation for socially vulnerable classes, including immigrant women and unemployed women, should be instigated.
Differences in the Prevalence of and Factors Associated with Frailty in Five Japanese Residential Areas
Authors: Abe Takumi , Kitamura Akihiko , Seino Satoshi , Yokoyama Yuri , Amano Hidenori , Taniguchi Yu , Nishi Mariko , Narita Miki , Ikeuchi Tomoko , Tomine Yui , Fujiwara Yoshinori , Shinkai Shoji
Published: October 2019

This study aimed to examine area differences in the prevalence of and factors associated with frailty. This cross-sectional study included metropolitan (eastern and western areas), suburban (districts A and B), and rural areas of Japan (n = 9182, woman 50.9%). Frailty was defined by using a standardized questionnaire comprising three subcategories (fall, nutritional status, and social activities). The prevalence of frailty in the five areas was 14.2% to 30.6% for men and 11.5% to 21.4% for women. The areas with a high frailty prevalence had a significantly lower nutritional status or social activity, or both. Compared to the western metropolitan area, among men, the multivariable-adjusted prevalence ratio (APR) of frailty was significantly higher in the eastern metropolitan area and lower in suburban district A, and among women, the eastern metropolitan and rural areas had significantly higher APRs. Area-stratified multiple Poisson regression analysis showed that age, bone and joint disease, and a subjective economic status were associated with frailty in most areas and that some factors were area-specific, i.e., living alone (for men living in metropolitan areas) and underweight (for women living in suburban areas). The frailty prevalence differed by area, even after multivariable adjustment. Area-specific characteristics and factors associated with frailty may result in area differences.
Burden and Anxiety in Family Caregivers in the Hospital That Debut in Caregiving
Authors: Pérez-Cruz Margarita , Parra-Anguita Laura , López-Martínez Catalina , Moreno-Cámara Sara , del-Pino-Casado Rafael
Published: October 2019

This cross-sectional study aims to determine the level of subjective burden and anxiety of caregivers of dependent older relatives that start providing care in the hospital and to analyse the relationship between objective burden, subjective burden and anxiety in these caregivers. Seventy-two caregivers of dependent older relatives were recruited in a medium–long stay hospital. Sociodemographic variables, number of basic activities of daily living (ADLs) attended, hours of surveillance, burden, and anxiety were collected from caregivers. A trajectory analysis was used to analyse the relationship between variables. Of the caregivers, 36.1% had subjective burden and 14.9% had anxiety. Subjective burden was positively associated with the number of basic ADLs attended, the hours of surveillance, and the cognitive impairment of the care recipient. Anxiety was also positively associated with subjective burden. Subjective burden mediated the effects of the number of basic ADLs attended, hours of surveillance and the cognitive impairment of the care recipient on anxiety. The levels of subjective burden and anxiety in caregivers debuting in hospital care are elevated, showing the need for these caregivers to be cared for. Subjective burden is a possible risk factor for anxiety, independent of the objective burden; it may buffer the effects of objective burden on anxiety.
Towards a People’s Social Epidemiology: Envisioning a More Inclusive and Equitable Future for Social Epi Research and Practice in the 21st Century
Authors: Petteway Ryan , Mujahid Mahasin , Allen Amani , Morello-Frosch Rachel
Published: October 2019

Social epidemiology has made critical contributions to understanding population health. However, translation of social epidemiology science into action remains a challenge, raising concerns about the impacts of the field beyond academia. With so much focus on issues related to social position, discrimination, racism, power, and privilege, there has been surprisingly little deliberation about the extent and value of social inclusion and equity within the field itself. Indeed, the challenge of translation/action might be more readily met through re-envisioning the role of the people within the research/practice enterprise—reimagining what “social” could, or even should, mean for the future of the field. A potential path forward rests at the nexus of social epidemiology, community-based participatory research (CBPR), and information and communication technology (ICT). Here, we draw from social epidemiology, CBPR, and ICT literatures to introduce A People’s Social Epi—a multi-tiered framework for guiding social epidemiology in becoming more inclusive, equitable, and actionable for 21st century practice. In presenting this framework, we suggest the value of taking participatory, collaborative approaches anchored in CBPR and ICT principles and technological affordances—especially within the context of place-based and environmental research. We believe that such approaches present opportunities to create a social epidemiology that is of, with, and by the people—not simply about them. In this spirit, we suggest 10 ICT tools to “socialize” social epidemiology and outline 10 ways to move towards A People’s Social Epi in practice.
Ecological Risk and Restoration Measures Relating to Heavy Metal Pollution in Industrial and Mining Wastelands
Authors: Cheng Huangxin , Huang Lei , Ma Pengtu , Shi Yi
Published: October 2019

In this study, we applied an integrated approach to an ecological risk evaluation of heavy metal pollution in industrial and mining wastelands in Yangxin County, China. A total of 72 sampling sites were designated in the study area. The results show that the potential ecological risk levels of Hg and Cd are higher, and the coefficient of variation of mercury levels is large. Cr, Cu, Zn, Pb, Ni, and As are all at low potential ecological risk. The land types with relatively high ecological risks are alum and coal mines. In the soil of alum mines, the risk due to mercury is higher, while in coal mine soil, the risk due to cadmium is relatively higher.
Natural Landscape, Infrastructure, and Health: The Physical Activity Implications of Urban Green Space Composition among the Elderly
Authors: Miralles-Guasch Carme , Dopico Javier , Delclòs-Alió Xavier , Knobel Pablo , Marquet Oriol , Maneja-Zaragoza Roser , Schipperijn Jasper , Vich Guillem
Published: October 2019

Urban green spaces (UGS) have been linked with a series of benefits for the environment, and for the physical health and well-being of urban residents. This is of great importance in the context of the aging of modern societies. However, UGS have different forms and characteristics that can determine their utilization. Common elements in UGS such as the type of vegetation and the type of surface are surprisingly understudied in regard to their relationship with the type of activity undertaken in UGS. This paper aims to explore the relationship between landscape diversity and the type of surface with the time spent and the physical activity intensity performed by seniors. To do so, this study uses GPS tracking data in combination with accelerometer data gathered from 63 seniors residing in Barcelona, Spain. Results showed that senior participants spent little time inside the analyzed UGS and sedentary behaviors (SBs) were more common than physical activities (PAs). The presence of pavement surfaces positively influenced the total time spent in UGS while gravel surfaces were negatively associated with time spent in active behaviors. The provision of well-defined and maintained paved areas and paths are some key infrastructures to be considered when designing UGS for overall urban residents and, especially, when aiming to potentiate the access for senior visitors.
The Prevalence of Overweight, Obesity, Hypertension, and Diabetes in India: Analysis of the 2015–2016 National Family Health Survey
Authors: Vennu Vishal , Abdulrahman Tariq A. , Bindawas Saad M.
Published: October 2019

Overweight, obesity, hypertension, and diabetes increase the risk of non-communicable diseases and all-cause mortality worldwide. Previous studies have not determined the prevalence of these conditions/diseases throughout India. Therefore, this study was aimed to address this limitation. Data on these conditions/diseases among men and women aged ≥ 18 years were obtained from the fourth National Family Health Survey conducted throughout India between January 2015 and December 2016. The prevalence and prevalence rate per 100,000 population were calculated at the national level and by age group, sex, and type of residence for each state and union territory. The national prevalence of overweight, obesity, hypertension, and diabetes were 14.6%, 3.4%, 5.2%, and 7.1%, respectively. The highest prevalence of these conditions/diseases at the national level was seen among those aged 35–49 years (54 years for men), especially women living in urban areas. In India, 1 out of every 7, 29, 19, and 14 individuals at the national level had overweight, obesity, hypertension, and diabetes, respectively—between 2015 and 2016. These results are important for the healthcare system and government policies in the future. Moreover, targeted efforts are required to establish public health strategies for the prevention, management, and treatment of these conditions/diseases throughout India.
Exploring the Impact of a Telehealth Care System on Organizational Capabilities and Organizational Performance from a Resource-Based Perspective
Authors: Chen Chun-Hsun , Lan Yu-Li , Yang Wei-Pang , Hsu Fang-Ming , Lin Chin-Lon , Chen Hsing-Chu
Published: October 2019

This study explored the effects of information technology (IT) resources—in conjunction with IT infrastructure and organizational resources—on organizational capabilities and performance. The study further analyzed the mediating effect of organizational capabilities on the relationship between IT resources and organizational performance. A cross-sectional research design was adopted, and questionnaire copies were administered to senior care supervisors of Taiwanese day care centers, care institutions, and hospitals. In total, 328 valid questionnaire responses were obtained. The study results are summarized as follows: (1) A direct effect analysis revealed that IT infrastructure significantly affected service performance and financial performance; organizational resources significantly affected service performance but did not significantly affect financial performance. (2) A mediation model analysis indicated that organizational capabilities exerted a mediating effect on the relationship between IT resources and organizational performance. These results can serve as a reference for medical care organizations in developing strategies for reviewing internal IT resources, integrating internal and external capabilities, creating a competitive advantage, and boosting their performance.
Role of Heat Shock Proteins in Glaucoma
Authors: Tsai Teresa , Grotegut Pia , Reinehr Sabrina , Joachim Stephanie C.
Published: October 2019

Glaucoma, one of the most common causes of blindness worldwide, is a multifactorial neurodegenerative disease characterized by damage of retinal ganglion cells and optic nerve degeneration. However, the exact mechanism leading to glaucoma is still not understood. Evidences suggest an immunological involvement in the pathogenesis. Among other immune responses, altered autoantibody patterns were found in glaucoma patients. Especially elevated antibody levels against heat shock proteins (HSPs), like HSP27 or HSP60, were identified. In an animal model, an immunization with these HSPs induced a pressure-independent retinal ganglion cell degeneration and axon loss, hence mimicking glaucoma-like damage. In addition, development of autoreactive antibodies, as well as a glia and T-cell activation, were described in these animals. Recently, we noted that intravitreal HSP27 injection likewise led to a degeneration of retinal ganglion cells and their axons. Therefore, HSP27 might have a direct damaging effect on retinal cells, and might play a key role in glaucoma.
The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts
Authors: Ma Jianyu , Ren Caifang , Yang Hua , Zhao Jie , Wang Feng , Wan Yongjie
Published: October 2019

The complement 1q binding protein C (C1QBP), also known as p32, is highly expressed in rapidly growing tissues and plays a crucial role in cell proliferation and apoptosis. However, there are no data interpreting its mechanisms in muscle development. To investigate the role of p32 in sheep muscle development, an 856 bp cDNA fragment of p32 containing an 837 bp coding sequence that encodes 278 amino acids was analyzed. We then revealed that the expression of p32 in the longissimus and quadricep muscles of fetal sheep was more significantly up-regulated than expression at other developmental stages. Furthermore, we found that the expression of p32 was increased during myoblasts differentiation in vitro. Additionally, the knockdown of p32 in sheep myoblasts effectively inhibited myoblast differentiation, proliferation, and promoted cell apoptosis in vitro. The interference of p32 also changed the energy metabolism from Oxidative Phosphorylation (OXPHOS) to glycolysis and activated AMP-activated protein kinase (AMPK) phosphorylation in sheep myoblasts in vitro. Taken together, our data suggest that p32 plays a vital role in the development of sheep muscle and provides a potential direction for future research on muscle development and some muscle diseases.
Detection of Antigen-Specific T Cells Using In Situ MHC Tetramer Staining
Authors: Abdelaal Hadia M. , Cartwright Emily K. , Skinner Pamela J.
Published: October 2019

The development of in situ major histocompatibility complex (MHC) tetramer (IST) staining to detect antigen (Ag)-specific T cells in tissues has radically revolutionized our knowledge of the local cellular immune response to viral and bacterial infections, cancers, and autoimmunity. IST combined with immunohistochemistry (IHC) enables determination of the location, abundance, and phenotype of T cells, as well as the characterization of Ag-specific T cells in a 3-dimensional space with respect to neighboring cells and specific tissue locations. In this review, we discuss the history of the development of IST combined with IHC. We describe various methods used for IST staining, including direct and indirect IST and IST performed on fresh, lightly fixed, frozen, and fresh then frozen tissue. We also describe current applications for IST in viral and bacterial infections, cancer, and autoimmunity. IST combined with IHC provides a valuable tool for studying and tracking the Ag-specific T cell immune response in tissues.
Functional Block Copolymers Carrying One Double-Stranded Ladderphane and One Single-Stranded Block in a Facile Metathesis Cyclopolymerization Procedure
Authors: Song Wei , Li Yadi , Liu Xunhu , Xu Zongyi , Wu Jianhua , Ding Liang
Published: October 2019

In order to improve the poor film-forming ability of polymeric ladderphane, di-block copolymers containing perylene diimide (PDI)-linked double-stranded poly(1,6–heptadiyne) ladderphane and branched alkyl side chains modified single-stranded poly(1,6–heptadiyne) were synthesized by metathesis cyclopolymerization (MCP) using Grubbs third-generation catalyst (Ru–III) in tetrahydrofuran solvent. The first block containing the ladderphane structure leads to higher thermal-stability, wider UV–vis absorption, lower LUMO level and ladderphane-induced rigidity and poor film-forming ability. The second block containing long alkyl chains is crucial for the guarantee of excellent film-forming ability. By comparing the effect of ladderphane structure on the resulted copolymers, single-stranded poly(1,6–heptadiyne) derivatives with PDI pedant were also processed. The structures of copolymers were proved by 1H NMR and gel permeation chromatography, electrochemical, photophysical, and thermal-stability performance were achieved by cyclic voltammetry (CV), UV-visible spectroscopy and thermogravimetric analysis (TGA) measurements. According to the experiment results, both copolymers possessed outstanding film-forming ability, which cannot be realized by small PDI molecules and oligomers. And they can serve as a superior candidate as for n-type materials, especially for their relatively wide range of light absorption (λ = 200~800 nm), and lower LUMO level (−4.3 and −4.0 eV).
Comparative Mitochondrial Genome Analysis of Two Ectomycorrhizal Fungi (Rhizopogon) Reveals Dynamic Changes of Intron and Phylogenetic Relationships of the Subphylum Agaricomycotina
Authors: Li Qiang , Ren Yuanhang , Shi Xiaodong , Peng Lianxin , Zhao Jianglin , Song Yu , Zhao Gang
Published: October 2019

In the present study, we assembled and compared two mitogenomes from the Rhizopogon genus. The two mitogenomes of R. salebrosus and R. vinicolor comprised circular DNA molecules, with the sizes of 66,704 bp and 77,109 bp, respectively. Comparative mitogenome analysis indicated that the length and base composition of protein coding genes (PCGs), rRNA genes and tRNA genes varied between the two species. Large fragments aligned between the mitochondrial and nuclear genomes of both R. salebrosus (43.41 kb) and R. vinicolor (12.83 kb) indicated that genetic transfer between mitochondrial and nuclear genomes has occurred over evolutionary time of Rhizopogon species. Intronic regions were found to be the main factors contributing to mitogenome expansion in R. vinicolor. Variations in the number and type of introns in the two mitogenomes indicated that frequent intron loss/gain events occurred during the evolution of Rhizopogon species. Phylogenetic analyses based on Bayesian inference (BI) and Maximum likelihood (ML) methods using a combined mitochondrial gene set yielded identical and well-supported tree topologies, wherein Rhizopogon species showed close relationships with Agaricales species. This is the first study of mitogenomes within the genus Rhizopogon, and it provides a basis for understanding the evolution and differentiation of mitogenomes from the ectomycorrhizal fungal genus.
Low Intensity Pulsed Ultrasound Prevents Recurrent Ischemic Stroke in a Cerebral Ischemia/Reperfusion Injury Mouse Model via Brain-derived Neurotrophic Factor Induction
Authors: Wu Cheng-Tien , Yang Ting-Hua , Chen Man-Chih , Chung Yao-Pang , Guan Siao-Syun , Long Lin-Hwa , Liu Shing-Hwa , Chen Chang-Mu
Published: October 2019

The incidence of stroke recurrence is still higher despite the advanced progression of therapeutic treatment and medical technology. Low intensity pulsed ultrasound (LIPUS) has been demonstrated to possess therapeutic effects on neuronal diseases and stroke via brain-derived neurotrophic factor (BDNF) induction. In this study, we hypothesized that LIPUS treatment possessed therapeutic benefits for the improvement of stroke recurrence. Adult male C57BL/6J mice were subjected to a middle cerebral artery occlusion (MCAO) surgery and then followed to secondary MCAO surgery as a stroke recurrence occurred after nine days from the first MCAO. LIPUS was administered continuously for nine days before secondary MCAO. LIPUS treatment not only decreased the mortality but also significantly moderated neuronal function injury including neurological score, motor activity, and brain pathological score in the recurrent stroke mice. Furthermore, the administration of LIPUS attenuated the apoptotic neuronal cells and increased Bax/Bcl-2 protein expression ratio and accelerated the expression of BDNF in the brain of the recurrent stroke mice. Taken together, these results demonstrate for the first time that LIPUS treatment arouses the expression of BDNF and possesses a therapeutic benefit for the improvement of stroke recurrence in a mouse model. The neuroprotective potential of LIPUS may provide a useful strategy for the prevention of a recurrent stroke.
Natural Products in Neurodegenerative Diseases: A Great Promise but an Ethical Challenge
Authors: Di Paolo Marco , Papi Luigi , Gori Federica , Turillazzi Emanuela
Published: October 2019

Neurodegenerative diseases (NDs) represent one of the most important public health problems and concerns, as they are a growing cause of mortality and morbidity worldwide, particularly in the elderly. Despite remarkable breakthroughs in our understanding of NDs, there has been little success in developing effective therapies. The use of natural products may offer great potential opportunities in the prevention and therapy of NDs; however, many clinical concerns have arisen regarding their use, mainly focusing on the lack of scientific support or evidence for their efficacy and patient safety. These clinical uncertainties raise critical questions from a bioethical and legal point of view, as considerations relating to patient decisional autonomy, patient safety, and beneficial or non-beneficial care may need to be addressed. This paper does not intend to advocate for or against the use of natural products, but to analyze the ethical framework of their use, with particular attention paid to the principles of biomedical ethics. In conclusion, the notable message that emerges is that natural products may represent a great promise for the treatment of many NDs, even if many unknown issues regarding the efficacy and safety of many natural products still remain.
AMPK Activation Promotes Tight Junction Assembly in Intestinal Epithelial Caco-2 Cells
Authors: Olivier Séverine , Leclerc Jocelyne , Grenier Adrien , Foretz Marc , Tamburini Jérôme , Viollet Benoit
Published: October 2019

The AMP-activated protein kinase (AMPK) is principally known as a major regulator of cellular energy status, but it has been recently shown to play a key structural role in cell-cell junctions. The aim of this study was to evaluate the impact of AMPK activation on the reassembly of tight junctions in intestinal epithelial Caco-2 cells. We generated Caco-2 cells invalidated for AMPK α1/α2 (AMPK dKO) by CRISPR/Cas9 technology and evaluated the effect of the direct AMPK activator 991 on the reassembly of tight junctions following a calcium switch assay. We analyzed the integrity of the epithelial barrier by measuring the trans-epithelial electrical resistance (TEER), the paracellular permeability, and quantification of zonula occludens 1 (ZO-1) deposit at plasma membrane by immunofluorescence. Here, we demonstrated that AMPK deletion induced a delay in tight junction reassembly and relocalization at the plasma membrane during calcium switch, leading to impairments in the establishment of TEER and paracellular permeability. We also showed that 991-induced AMPK activation accelerated the reassembly and reorganization of tight junctions, improved the development of TEER and paracellular permeability after calcium switch. Thus, our results show that AMPK activation ensures a better recovery of epithelial barrier function following injury.