bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Soluble Solids and High Pressure Treatment on Rheological Properties of Protein Enriched Mango Puree
Authors: Ramaswamy Hosahalli S. , Gundurao Anuradha
Published: January 2019

The effects of high pressure treatment on the rheological properties of protein enriched mango puree were evaluated. In the first part, the original soluble solids in mango puree (28° Brix) was lowered to 26, 23 and 20° Brix, and each was supplemented with 2, 5 and 8% of whey protein to assess the influence of added protein. The samples were then evaluated by dynamic rheology. Pressure treatment resulted in a progressive increase in elastic (G′) and viscous modulii (G″) as well as complex viscosity (η*). Values of G′ were higher than G″ demonstrating that the product behaved more like a gel. Additional tests were carried out by simultaneously varying protein and soluble solids contents, and keeping the total solids at 28%. In this case, the effects of pressure levels and holding times were similar to previous results. However, the positive effects of higher protein were negated by the negative effects of lower soluble solids, resulting in an overall decreasing effect on rheology. The developed models effectively predicted the combined influence of protein and soluble solid concentrations on rheological parameters (R2 > 0.85). Sensory evaluation of 2% and 5% protein supplemented and pressure treated (500 MPa/3 min) mango puree yielded acceptable sensory qualities, resulting in a product with enriched protein content.
Genome-Wide Identification and Expression Profiling of Sugar Transporter Protein (STP) Family Genes in Cabbage (Brassica oleracea var. capitata L.) Reveals their Involvement in Clubroot Disease Responses
Authors: Zhang Wei , Wang Shenyun , Yu Fangwei , Tang Jun , Yu Li , Wang Hong , Li Jianbin
Published: January 2019

Sugar transporter protein (STP) genes are involved in multiple biological processes, such as plant responses to various stresses. However, systematic analysis and functional information of STP family genes in Brassica oleracea are very limited. A comprehensive analysis was carried out to identify BoSTP genes and dissect their phylogenetic relationships and to investigate the expression profiles in different organs and in response to the clubroot disease. A total of 22 BoSTP genes were identified in the B. oleracea genome and they were further classified into four clades based on the phylogenetic analysis. All the BoSTP proteins harbored the conserved sugar transporter (Sugar_tr, PF00083) domain, and the majority of them contained 12 transmembrane helices (TMHs). Rates of synonymous substitution in B. oleracea relative to Arabidopsis thaliana indicated that STP genes of B. oleracea diverged from those of A. thaliana approximately 16.3 million years ago. Expression profiles of the BoSTP genes in different organs derived from RNA-Seq data indicated that a large number of the BoSTP genes were expressed in specific organs. Additionally, the expression of BoSTP4b and BoSTP12 genes were induced in roots of the clubroot-susceptible cabbage (CS-JF1) at 28 days after inoculation with Plasmodiophora brassicae, compared with mock-inoculated plants. We speculated that the two BoSTPs might be involved in monosaccharide unloading and carbon partitioning associated with P. brassicae colonization in CS-JF1. Subcellular localization analysis indicated that the two BoSTP proteins were localized in the cell membrane. This study provides insights into the evolution and potential functions of BoSTPs.
Impacts of Earthquakes on Energy Security in the Eurasian Economic Union: Resilience of the Electricity Transmission Networks in Russia, Kazakhstan, and Kyrgyzstan
Authors: Iakubovskii Dmitrii , Komendantova Nadejda , Rovenskaya Elena , Krupenev Dmitry , Boyarkin Denis
Published: January 2019

In our research, we focus on the reliability of the interconnected electricity supply system of three countries of the Eurasian Economic Union (EAEU)—Russia, Kazakhstan, and Kyrgyzstan. We apply a mathematical model to evaluate the reliability of the electricity supply system under the threat of earthquakes. Earthquakes can damage elements of electricity grids and, considering the interconnectivity of electricity supply systems in the EAEU, effects in the aftermath of earthquakes can be far-reaching and even transboundary. This necessitates the development of coordinated policies and risk management strategies to deal with electricity outage risks in the EAEU. In our study, the earthquake probability is derived from seismic zone maps, while damage events are computed using maps of energy power systems. In addition, we determine which elements of the system are susceptible to failure due to an earthquake of a given magnitude. We conduct a scenario analysis of earthquakes and their impacts on the reliability of the power supply system, considering potential energy losses and threats to energy security. An analysis of the resilience of electricity transmission grids allows us to determine the critical interconnection lines in terms of exposure to earthquake risk, as well as exposure to total systemic loss. We also identify the most critical interconnection lines where power outages can lead to the destabilization of the entire power supply system. Some examples of such lines are at the border of Kazakhstan and Kyrgyzstan, where power outages can lead to serious economic costs and electricity outages.
An Improved Strength Reduction-Based Slope Stability Analysis
Authors: Seyed-Kolbadi S. M. , Sadoghi-Yazdi J. , Hariri-Ardebili M. A.
Published: January 2019

Slope uncertainty predominantly originates from the imperfect analysis model and the inaccuracy and imprecision of the observations. The strength reduction method (SRM) is widely used to attain the safety factor (SF) of the slopes, which is similar to interpretation of the limit state (LS). In this paper, the spectral element method (SEM), using an elasto-plastic Mohr–Coulomb failure criterion, is employed to project the plausible LS of the soil slopes. An iterative SRM search method is proposed to evaluate the SF of the slopes regardless of the LS interpretation. The proposed SRM paradigm encompasses the design trigger to trace the uncertain parameters in decision-making. This method is applied to three numerical examples: (1) a homogeneous dry slope, (2) a dry slope with a weak layer, and (3) a partially-wet slope with a weak layer. It is shown that for the case study examples, the proposed SRM reasonably converges to the required precision. Results further are compared and contrasted with some of the conventional and standard techniques in slope stability. This hybrid procedure paves the road for fast and safe stability analysis of man-made and natural slopes.
Greenhouse Soil Biosolarization with Tomato Plant Debris as a Unique Fertilizer for Tomato Crops
Authors: García-Raya Pablo , Ruiz-Olmos César , Marín-Guirao José Ignacio , Asensio-Grima Carlos , Tello-Marquina Julio César , de Cara-García Miguel
Published: January 2019

Intensive greenhouse horticulture can cause various environmental problems. Among these, the management, storage, and processing of crop residues can provoke aquifer contamination, pest proliferation, bad odors, or the abuse of phytosanitary treatments. Biosolarization adds value to any fresh plant residue and is an efficient technique for the control of soil-borne diseases. This study aims to examine an alternative means of managing greenhouse crop residues through biosolarization and to investigate the influence of organic matter on yield and quality of tomato (Solanum lycopersicum, L.) fruit. With this purpose, the following nutritional systems were evaluated: inorganic fertilization with and without brassica pellets (Fert, Fert +, and Fert ++), fresh tomato plant debris with and without brassica pellets (Rest, Rest +, and Rest ++), and no fertilizer application (Control). The addition of organic matter was equal across all the treatments except for the control with regard to yield and quality of the tomato fruit. In light of these results, the application of tomato plant debris to the soil through biosolarization is postulated as an alternative for the management of crop residues, solving an environmental problem and having a favorable impact on the production and quality of tomatoes as a commercial crop.
Study on Lifestyle Evaluation Systems for the Health of Chinese Elderly
Authors: Du Changliang
Published: January 2019

Background/Objective: China is now faced with a serious population aging challenge, and the health of the Chinese elderly is becoming an imminent concern. Consequently, it is critical to establish a lifestyle evaluation system for promoting the health of the Chinese elderly. Methods: Interviews with experts and questionnaire surveys were conducted. Factor analysis, analytic hierarchy process, and statistical analyses were also adopted in this study. Results: Besides evaluation metrics and standards, a two-level category system including 50 indices and associated weights from three level 1 categories (physical and mental health and social wellbeing) and thirteen level 2 categories were obtained. Discussion and Conclusions: Based on the confirmatory factor analysis and Cronbach’s test, such an evaluation system excels in effectiveness and reliability, and is ready to be popularized in Chinese society. We expect that the Chinese elderly will benefit from our system and that it will lead to a healthy lifestyle accordingly.
Heavy Metal Concentrations in the Groundwater of the Barcellona-Milazzo Plain (Italy): Contributions from Geogenic and Anthropogenic Sources
Authors: Cangemi Marianna , Madonia Paolo , Albano Ludovico , Bonfardeci Alessandro , Di Figlia Maria Grazia , Di Martino Roberto Maria Rosario , Nicolosi Marco , Favara Rocco
Published: January 2019

We collected and analysed 58 samples of groundwater from wells in the Barcellona-Milazzo Plain, one of the most important coastal aquifers of Sicily (Italy), to determine major, minor, and trace element concentrations. In this area, geogenic and anthropogenic sources of heavy metals and other pollutants co-act, making the individuation of the main pollution sources difficult. Our work was aimed at the application of geostatistical criteria for discriminating between these pollution sources. We used probability plots for separating anomalous values from background concentrations, which were plotted on maps and related to possible sources of pollutants. Our results indicate that hydrothermal fluid circulation and the water–rock interaction of country rocks that host mineralized ore deposits generate a significant flux of heavy metals to groundwater, as well as anthropogenic sources like intense agriculture and industrial activities. In particular, NO3, F, and Ni exceed the Maximum Admitted Concentrations (MACs) established by the WHO and Italian legislation for drinking-water. The spatial distributions of geogenic and anthropogenic sources were so deeply interlocked that their separation was not easy, also employing geostatistical tools. This complex scenario makes the implementation of human health risk mitigation actions difficult, since the flow of pollutants is in many cases controlled by simple water–rock interaction processes.
Differences in Insomnia Symptoms between Immigrants and Non-Immigrants in Switzerland attributed to Emotional Distress: Analysis of the Swiss Health Survey
Authors: Schneeberger Andres R. , Seixas Azizi , Schweinfurth Nina , Lang Undine E. , Cajochen Christian , Bux Donald A. , Richards Shannique , Jean-Louis Girardin , Huber Christian G.
Published: January 2019

Migration can be a stressful experience and may lead to poor health and behavioral changes. The immigrant population in Switzerland is disproportionately burdened by several negative health outcomes, chief among these is mental health issues. The aim of the study was to investigate whether sleep disturbances are more prevalent among immigrants compared to non-immigrants and whether emotional distress might explain sleep differences. Based on the Swiss Health Survey 2012 dataset, we analyzed the data of 17,968 people, of which 3406 respondents were immigrants. We examined variables including insomnia symptoms, emotional distress and clinical and socio-demographic data using unadjusted and adjusted generalized linear models. Compared to non-immigrants, immigrants suffer significantly more often from insomnia symptoms. Immigrants also endured higher levels of emotional distress. Higher values of emotional distress are related to other symptoms of sleep disorders. Immigrants with emotional distress were at significant risk of sleep disturbances. Sleep disparities between immigrants and non-immigrants may be influenced by emotional distress. Migration health care should address emotional distress, a more proximal and modifiable factor, as a possible cause of insomnia symptoms in immigrants.
The Role of Anthropogenic Elements in the Environment for Affective States and Cortisol Concentration in Mountain Hiking—A Crossover Trial
Authors: Niedermeier Martin , Grafetstätter Carina , Kopp Martin , Huber Daniela , Mayr Michaela , Pichler Christina , Hartl Arnulf
Published: January 2019

Green exercise might have positive effects on health and affective states. Little is known about the ideal characteristics of the natural environment, where exercise is conducted in. Thus, the primary aim of the present study was to investigate the effects of anthropogenic elements on acute stress-related physiological responses and affective states in green exercise. Using a crossover field study design, 52 healthy participants were exposed to two different mountain hiking conditions: An environment with less anthropogenic elements and an environment with more anthropogenic elements. Pre and post conditions, affective states and salivary cortisol concentration were measured. Repeated measures ANOVAs were used to analyze if pre-post changes differed between the conditions. Pre-post changes in affective states and salivary cortisol concentration did not significantly differ, partial η² < 0.06. Positive affective states showed significantly higher values post compared to pre-condition, partial η² > 0.13. The present results indicate that anthropogenic elements have a minor role in the influence on affective states and salivary cortisol concentration during mountain hiking. It is concluded that a single bout of mountain hiking independent of anthropogenic elements in the environment is effective in influencing affective states positively.
SleepOMICS: How Big Data Can Revolutionize Sleep Science
Authors: Bragazzi Nicola Luigi , Guglielmi Ottavia , Garbarino Sergio
Published: January 2019

Sleep disorders have reached epidemic proportions worldwide, affecting the youth as well as the elderly, crossing the entire lifespan in both developed and developing countries. “Real-life” behavioral (sensor-based), molecular, digital, and epidemiological big data represent a source of an impressive wealth of information that can be exploited in order to advance the field of sleep research. It can be anticipated that big data will have a profound impact, potentially enabling the dissection of differences and oscillations in sleep dynamics and architecture at the individual level (“sleepOMICS”), thus paving the way for a targeted, “one-size-does-not-fit-all” management of sleep disorders (“precision sleep medicine”).
Restorative Effects of Classroom Soundscapes on Children’s Cognitive Performance
Authors: Shu Shan , Ma Hui
Published: January 2019

Previous studies have examined the restorative benefits of soundscapes on adults’ cognitive performance, but it was unclear whether those benefits would be possible for children. In this paper, two experiments applied a before–after design to explore the restorative effects of different soundscapes on children’s sustained attention and short-term memory, respectively, in a simulated classroom situation. In Experiment 1, 46 children aged 8–12 were first mentally fatigued by performing an oral arithmetic task and then were asked to conduct a sustained attention to response test (SART), in order to assess their attention fatigue. After that, a period of 3-min soundscape was presented, and SART was conducted again to examine their attention recovery. In Experiment 2, 45 children participated and the experiment procedure was the same as in Experiment 1, except that a digit span test (DST) was used instead to measure short-term memory. The results showed that music, birdsong, fountain sound, and stream sound facilitated greater recovery than other sounds in reaction time. Participants also showed better performance in short-term memory after exposure to fountain sound and stream sound, followed by music and birdsong. Those results confirmed the actual restorative effects of perceived restorative soundscapes on children’s cognitive performance.
Antifouling (Bio)materials for Electrochemical (Bio)sensing
Authors: Campuzano Susana , Pedrero María , Yáñez-Sedeño Paloma , Pingarrón José M.
Published: January 2019

(Bio)fouling processes arising from nonspecific adsorption of biological materials (mainly proteins but also cells and oligonucleotides), reaction products of neurotransmitters oxidation, and precipitation/polymerization of phenolic compounds, have detrimental effects on reliable electrochemical (bio)sensing of relevant analytes and markers either directly or after prolonged incubation in rich-proteins samples or at extreme pH values. Therefore, the design of antifouling (bio)sensing interfaces capable to minimize these undesired processes is a substantial outstanding challenge in electrochemical biosensing. For this purpose, efficient antifouling strategies involving the use of carbon materials, metallic nanoparticles, catalytic redox couples, nanoporous electrodes, electrochemical activation, and (bio)materials have been proposed so far. In this article, biomaterial-based strategies involving polymers, hydrogels, peptides, and thiolated self-assembled monolayers are reviewed and critically discussed. The reported strategies have been shown to be successful to overcome (bio)fouling in a diverse range of relevant practical applications. We highlight recent examples for the reliable sensing of particularly fouling analytes and direct/continuous operation in complex biofluids or harsh environments. Opportunities, unmet challenges, and future prospects in this field are also pointed out.
Multifaceted Role of PheDof12-1 in the Regulation of Flowering Time and Abiotic Stress Responses in Moso Bamboo (Phyllostachys edulis)
Authors: Liu Jun , Cheng Zhanchao , Xie Lihua , Li Xiangyu , Gao Jian
Published: January 2019

DNA binding with one finger (Dof) proteins, forming an important transcriptional factor family, are involved in gene transcriptional regulation, development, stress responses, and flowering responses in annual plants. However, knowledge of Dofs in perennial and erratically flowering moso bamboo is limited. In view of this, a Dof gene, PheDof12-1, was isolated from moso bamboo. PheDof12-1 is located in the nucleus and has the highest expression in palea and the lowest in bract. Moreover, PheDof12-1 expression is high in flowering leaves, then declines during flower development. The transcription level of PheDof12-1 is highly induced by cold, drought, salt, and gibberellin A3 (GA3) stresses. The functional characteristics of PheDof are researched for the first time in Arabidopsis, and the results show that transgenic Arabidopsis overexpressing PheDof12-1 shows early flowering under long-day (LD) conditions but there is no effect on flowering time under short-day (SD) conditions; the transcription levels of FT, SOC1, and AGL24 are upregulated; and FLC and SVP are downregulated. PheDof12-1 exhibits a strong diurnal rhythm, inhibited by light treatment and induced in dark. Yeast one-hybrid (Y1H) assay shows that PheDof12-1 can bind to the promoter sequence of PheCOL4. Taken together, these results indicate that PheDof12-1 might be involved in abiotic stress and flowering time, which makes it an important candidate gene for studying the molecular regulation mechanisms of moso bamboo flowering.
BAP1 Status Determines the Sensitivity of Malignant Mesothelioma Cells to Gemcitabine Treatment
Authors: Guazzelli Alice , Meysami Parisa , Bakker Emyr , Demonacos Constantinos , Giordano Antonio , Krstic-Demonacos Marija , Mutti Luciano
Published: January 2019

Malignant mesothelioma (MMe) is a cancer with poor prognosis and resistance to standard treatments. Recent reports have highlighted the role of the BRCA1 associated protein 1 gene (BAP1) in the development of MMe. In this study, the chemosensitivity of human mesothelioma cell lines carrying BAP1 wild-type (WT), mutant and silenced was analysed. The BAP1 mutant cells were significantly less sensitive than BAP1 WT cell lines to the clinically relevant drug gemcitabine. Silencing of BAP1 significantly increased resistance of MMe cells to gemcitabine. Cell cycle analysis suggested that gemcitabine induced Sub-G1 phase accumulation of the BAP1 WT cells and increased in the S-phase in both BAP1 WT and mutant cells. Analysis of the role of BAP1 in apoptosis suggested that gemcitabine induced early apoptosis in both BAP1 WT and BAP1 mutant cells but with a much higher degree in the WT cells. Effects on the population of cells in late apoptosis, which can mark necrosis and necroptosis, could not be seen in the mutant cells, highlighting the possibility that BAP1 plays a role in several types of cell death. Significantly decreased DNA damage in the form of double-strand breaks was observed in gemcitabine-treated BAP1 mutant cells, compared to BAP1 WT cells under the same conditions. After BAP1 silencing, a significant decrease in DNA damage in the form of double-strand breaks was observed compared to cells transfected with scramble siRNA. Taken together, the results presented in this manuscript shed light on the role of BAP1 in the response of MMe cells to gemcitabine treatment and in particular in the control of the DNA damage response, therefore providing a potential route for more efficient MMe therapy.
Current Trends in Fabrication of Biomaterials for Bone and Cartilage Regeneration: Materials Modifications and Biophysical Stimulations
Authors: Przekora Agata
Published: January 2019

The aim of engineering of biomaterials is to fabricate implantable biocompatible scaffold that would accelerate regeneration of the tissue and ideally protect the wound against biodevice-related infections, which may cause prolonged inflammation and biomaterial failure. To obtain antimicrobial and highly biocompatible scaffolds promoting cell adhesion and growth, materials scientists are still searching for novel modifications of biomaterials. This review presents current trends in the field of engineering of biomaterials concerning application of various modifications and biophysical stimulation of scaffolds to obtain implants allowing for fast regeneration process of bone and cartilage as well as providing long-lasting antimicrobial protection at the site of injury. The article describes metal ion and plasma modifications of biomaterials as well as post-surgery external stimulations of implants with ultrasound and magnetic field, providing accelerated regeneration process. Finally, the review summarizes recent findings concerning the use of piezoelectric biomaterials in regenerative medicine.
Antibacterial Composites of Cuprous Oxide Nanoparticles and Polyethylene
Authors: Gurianov Yanna , Nakonechny Faina , Albo Yael , Nisnevitch Marina
Published: January 2019

Cuprous oxide nanoparticles (Cu2ONPs) were used for preparing composites with linear low-density polyethylene (LLDPE) by co-extrusion, thermal adhesion, and attachment using ethyl cyanoacrylate, trimethoxyvinylsilane, and epoxy resin. The composites were examined by Scanning electron microscope and tested for their antibacterial activity against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. All of these composites—except for the one obtained by extrusion—eradicated cells of both bacteria within half an hour. The composite prepared by thermal adhesion of Cu2ONPs on LLDPE had the highest external exposure of nanoparticles and exhibited the highest activity against the bacteria. This composite and the one obtained using ethyl cyanoacrylate showed no leaching of copper ions into the aqueous phase. Copper ion leaching from composites prepared with trimethoxyvinylsilane and epoxy resin was very low. The antibacterial activity of the composites can be rated as follows: obtained by thermal adhesion > obtained using ethyl cyanoacrylate > obtained using trimethoxyvinylsilane > obtained using epoxy resin > obtained by extrusion. The composites with the highest activity are potential materials for tap water and wastewater disinfection.
Arf GAPs as Regulators of the Actin Cytoskeleton—An Update
Authors: Tanna Christine E. , Goss Louisa B. , Ludwig Calvin G. , Chen Pei-Wen
Published: January 2019

Arf GTPase-activating proteins (Arf GAPs) control the activity of ADP-ribosylation factors (Arfs) by inducing GTP hydrolysis and participate in a diverse array of cellular functions both through mechanisms that are dependent on and independent of their Arf GAP activity. A number of these functions hinge on the remodeling of actin filaments. Accordingly, some of the effects exerted by Arf GAPs involve proteins known to engage in regulation of the actin dynamics and architecture, such as Rho family proteins and nonmuscle myosin 2. Circular dorsal ruffles (CDRs), podosomes, invadopodia, lamellipodia, stress fibers and focal adhesions are among the actin-based structures regulated by Arf GAPs. Arf GAPs are thus important actors in broad functions like adhesion and motility, as well as the specialized functions of bone resorption, neurite outgrowth, and pathogen internalization by immune cells. Arf GAPs, with their multiple protein-protein interactions, membrane-binding domains and sites for post-translational modification, are good candidates for linking the changes in actin to the membrane. The findings discussed depict a family of proteins with a critical role in regulating actin dynamics to enable proper cell function.
Recurrent Stimulation of Natural Killer Cell Clones with K562 Expressing Membrane-Bound Interleukin-21 Affects Their Phenotype, Interferon-γ Production, and Lifespan
Authors: Streltsova Maria A. , Erokhina Sofya A. , Kanevskiy Leonid M. , Grechikhina Maria V. , Kobyzeva Polina A. , Lee Dean A. , Telford William G. , Sapozhnikov Alexander M. , Kovalenko Elena I.
Published: January 2019

A pattern of natural killer cell (NK cell) heterogeneity determines proliferative and functional responses to activating stimuli in individuals. Obtaining the progeny of a single cell by cloning the original population is one of the ways to study NK cell heterogeneity. In this work, we sorted single cells into a plate and stimulated them via interleukin (IL)-2 and gene-modified K562 feeder cells that expressed membrane-bound IL-21 (K562-mbIL21), which led to a generation of phenotypically confirmed and functionally active NK cell clones. Next, we applied two models of clone cultivation, which differently affected their phenotype, lifespan, and functional activity. The first model, which included weekly restimulation of clones with K562-mbIL21 and IL-2, resulted in the generation of relatively short-lived (5–7 weeks) clones of highly activated NK cells. Levels of human leukocyte antigen class II molecule—DR isotype (HLA-DR) expression in the expanded NK cells correlated strongly with interferon-γ (IFN-γ) production. The second model, in which NK cells were restimulated weekly with IL-2 alone and once on the sixth week with K562-mbIL21 and IL-2, produced long-lived clones (8–14 weeks) that expanded up to 107 cells with a lower ability to produce IFN-γ. Our method is applicable for studying variability in phenotype, proliferative, and functional activity of certain NK cell progeny in response to the stimulation, which may help in selecting NK cells best suited for clinical use.
Clinical Use and Molecular Action of Corticosteroids in the Pediatric Age
Authors: Ferrara Giovanna , Petrillo Maria Grazia , Giani Teresa , Marrani Edoardo , Filippeschi Cesare , Oranges Teresa , Simonini Gabriele , Cimaz Rolando
Published: January 2019

Corticosteroids are the mainstay of therapy for many pediatric disorders and sometimes are life-saving. Both endogenous and synthetic derivatives diffuse across the cell membrane and, by binding to their cognate glucocorticoid receptor, modulate a variety of physiological functions, such as glucose metabolism, immune homeostasis, organ development, and the endocrine system. However, despite their proved and known efficacy, corticosteroids show a lot of side effects, among which growth retardation is of particular concern and specific for pediatric age. The aim of this review is to discuss the mechanism of action of corticosteroids, and how their genomic effects have both beneficial and adverse consequences. We will focus on the use of corticosteroids in different pediatric subspecialties and most common diseases, analyzing the most recent evidence.
Hydrogen Indirectly Suppresses Increases in Hydrogen Peroxide in Cytoplasmic Hydroxyl Radical-Induced Cells and Suppresses Cellular Senescence
Authors: Sakai Takahiro , Kurokawa Ryosuke , Hirano Shin-ichi , Imai Jun
Published: January 2019

Bacteria inhabiting the human gut metabolize microbiota-accessible carbohydrates (MAC) contained in plant fibers and subsequently release metabolic products. Gut bacteria produce hydrogen (H2), which scavenges the hydroxyl radical (•OH). Because H2 diffuses within the cell, it is hypothesized that H2 scavenges cytoplasmic •OH (cyto •OH) and suppresses cellular senescence. However, the mechanisms of cyto •OH-induced cellular senescence and the physiological role of gut bacteria-secreted H2 have not been elucidated. Based on the pyocyanin-stimulated cyto •OH-induced cellular senescence model, the mechanism by which cyto •OH causes cellular senescence was investigated by adding a supersaturated concentration of H2 into the cell culture medium. Cyto •OH-generated lipid peroxide caused glutathione (GSH) and heme shortage, increased hydrogen peroxide (H2O2), and induced cellular senescence via the phosphorylation of ataxia telangiectasia mutated kinase serine 1981 (p-ATMser1981)/p53 serine 15 (p-p53ser15)/p21 and phosphorylation of heme-regulated inhibitor (p-HRI)/phospho-eukaryotic translation initiation factor 2 subunit alpha serine 51 (p-eIF2α)/activating transcription factor 4 (ATF4)/p16 pathways. Further, H2 suppressed increased H2O2 by suppressing cyto •OH-mediated lipid peroxide formation and cellular senescence induction via two pathways. H2 produced by gut bacteria diffuses throughout the body to scavenge cyto •OH in cells. Therefore, it is highly likely that gut bacteria-produced H2 is involved in intracellular maintenance of the redox state, thereby suppressing cellular senescence and individual aging. Hence, H2 produced by intestinal bacteria may be involved in the suppression of aging.
Architecture of a Dispersed Gamification System for Tourist Attractions
Authors: Swacha Jakub
Published: January 2019

The paper addresses the issues of implementing e-guide-based gamification systems for tourist attractions by proposing a dispersed gamification system architecture in which its respective functional aspects are handled by multiple web services with various scopes of responsibility. The features provided by the proposed architecture are successfully matched to tourist attraction requirements identified during development of an e-guide gamification system for an international consortium of tourist attractions. The key contributions of the paper are: the new definition of gamification system extending beyond the concept of a set of game-based rules and including the information technology components necessary to implement it; mapping the abstract gamification system model into the domain of tourist attraction gamification and the analysis of advantages and disadvantages of various organizations of e-guide gamification systems; the introduction of a new, dispersed architecture of e-guide gamification system that addresses the identified needs of tourist attractions.
Ecological Drivers of Species Distributions and Niche Overlap for Three Subterranean Termite Species in the Southern Appalachian Mountains, USA
Authors: Hyseni Chaz , Garrick Ryan
Published: January 2019

In both managed and unmanaged forests, termites are functionally important members of the dead-wood-associated (saproxylic) insect community. However, little is known about regional-scale environmental drivers of geographic distributions of termite species, and how these environmental factors impact co-occurrence among congeneric species. Here we focus on the southern Appalachian Mountains—a well-known center of endemism for forest biota—and use Ecological Niche Modeling (ENM) to examine the distributions of three species of Reticulitermes termites (i.e., R. flavipes, R. virginicus, and R. malletei). To overcome deficiencies in public databases, ENMs were underpinned by field-collected high-resolution occurrence records coupled with molecular taxonomic species identification. Spatial overlap among areas of predicted occurrence of each species was mapped, and aspects of niche similarity were quantified. We also identified environmental factors that most strongly contribute to among-species differences in occupancy. Overall, we found that R. flavipes and R. virginicus showed significant niche divergence, which was primarily driven by summer temperature. Also, all three species were most likely to co-occur in the mid-latitudes of the study area (i.e., northern Alabama and Georgia, eastern Tennessee and western North Carolina), which is an area of considerable topographic complexity. This work provides important baseline information for follow-up studies of local-scale drivers of these species’ distributions. It also identifies specific geographic areas where future assessments of the frequency of true syntopy vs. micro-allopatry, and associated interspecific competitive interactions, should be focused.
RETRACTED: Bruder et al. Transcatheter Decellularized Tissue-Engineered Heart Valve (dTEHV) Grown on Polyglycolic Acid (PGA) Scaffold Coated with P4HB Shows Improved Functionality over 52 Weeks due to Polyether-Ether-Ketone (PEEK) Insert. J. Funct. Biomater. 2018, 9, 64
Authors: Bruder Leon , Brakmann Kerstin , Stegner Valentin , Sigler Matthias , Berger Felix , Schmitt Boris
Published: January 2019

Due to human error, the authors included some of the experimental data in this article [...]
Detection Accuracy of Soccer Players in Aerial Images Captured from Several Viewpoints
Authors: Oki Takuro , Miyamoto Ryusuke , Yomo Hiroyuki , Hara Shinsuke
Published: January 2019

In the fields of professional and amateur sports, players’ health, physical and physiological conditions during exercise should be properly monitored and managed. The authors of this paper previously proposed a real-time vital-sign monitoring system for players using a wireless multi-hop sensor network that transmits their vital data. However, existing routing schemes based on the received signal strength indicator or global positioning system do not work well, because of the high speeds and the density of sensor nodes attached to players. To solve this problem, we proposed a novel scheme, image-assisted routing (IAR), which estimates the locations of sensor nodes using images captured from cameras mounted on unmanned aerial vehicles. However, it is not clear where the best viewpoints are for aerial player detection. In this study, the authors investigated detection accuracy from several viewpoints using an aerial-image dataset generated with computer graphics. Experimental results show that the detection accuracy was best when the viewpoints were slightly distant from just above the center of the field. In the best case, the detection accuracy was very good: 0.005524 miss rate at 0.01 false positive-per-image. These results are informative for player detection using aerial images and can facilitate to realize IAR.
Power and Area Efficient Clock Stretching and Critical Path Reshaping for Error Resilience
Authors: Jayakrishnan Mini , Chang Alan , Kim Tony Tae-Hyoung
Published: January 2019

Energy efficient semiconductor chips are in high demand to cater the needs of today’s smart products. Advanced technology nodes insert high design margins to deal with rising variations at the cost of power, area and performance. Existing run time resilience techniques are not cost effective due to the additional circuits involved. In this paper, we propose a design time resilience technique using a clock stretched flip-flop to redistribute the available slack in the processor pipeline to the critical paths. We use the opportunistic slack to redesign the critical fan in logic using logic reshaping, better than worst case sigma corner libraries and multi-bit flip-flops to achieve power and area savings. Experimental results prove that we can tune the logic and the library to get significant power and area savings of 69% and 15% in the execute pipeline stage of the processor compared to the traditional worst-case design. Whereas, existing run time resilience hardware results in 36% and 2% power and area overhead respectively.
Analysis of Melt Pool Characteristics and Process Parameters Using a Coaxial Monitoring System during Directed Energy Deposition in Additive Manufacturing
Authors: Kledwig Christian , Perfahl Holger , Reisacher Martin , Brückner Frank , Bliedtner Jens , Leyens Christoph
Published: January 2019

The growing number of commercially available machines for laser deposition welding show the growing acceptance and importance of this technology for industrial applications. Their increasing usage in research and production requires process stability and user-friendly handling. A commercially available DMG MORI LT 65 3D hybrid machine used in combination with a CCD-based coaxial temperature measurement system was utilized in this work to investigate what information relating to the intensity distribution of melt pool surfaces could be appropriate to draw conclusions about process conditions. In this study it is shown how the minimal required specific energy for a stable process can be determined, and it is indicated that the evolution of a plasma plume depends on thermal energy within the base material. An estimated melt pool area—calculated by the number of pixels (NOP) with intensities larger than a fixed, predefined threshold—builds the main measure in analysing images from the process camera. The melt pool area and its temporal variance can also serve as an indicator for an increased working distance.
Effect of Lubricating Phase on Microstructure and Properties of Cu–Fe Friction Materials
Authors: Wang Xiaoyang , Ru Hongqiang
Published: January 2019

Cu–Fe-based friction materials with flake graphite, granulated carbon black, and high-strength graphite as lubricating phase were prepared by the powder metallurgy method. The effects of different types and mass fraction of lubricating phase on the microstructure, mechanical properties, and tribological properties were investigated. The results show that when the mass fraction of granulated carbon black is 5 wt%, it is easy to form a good interface with the matrix, but the interface is prone to pores and cracks when its mass fraction is 10 wt%. The bending strength and compressive strength properties of the composites increased with increasing in the mass fraction of granulated carbon black and reached the maximum of 40 MPa and 70 MPa at 5 wt% granulated carbon black, after which bending strength and compressive strength all decreased. The friction coefficient and the wear loss of the materials initially decreased as the mass fraction of granulated carbon black increased and obtained minimum of 0.436 and 0.145 mm when the mass fraction of granulated carbon black was 5 wt%, then ascended. Compared with the sample with 5 wt% high-strength graphite as lubricating phase, the sample with 5 wt% granulated carbon black as lubricating phase had better sintering performance, mechanical properties, and tribological properties.
Setting Characteristics, Mechanical Properties and Microstructure of Cement Pastes Containing Accelerators Mixed with Superabsorbent Polymers (SAPs): An NMR Study Combined with Additional Methods
Authors: Ji Yanliang , Sun Zhenping , Chen Chao , Pel Leo , Barakat Ahmed
Published: January 2019

In this study, the setting property and mechanical strength of cement pastes containing accelerators (CPCA) with or without superabsorbent polymers (SAPs) were first studied. The early microstructure evolution and water distribution at 7 and 28 days were probed by 1D (T2) and 2D (T1-T2 maps) H1 nuclear magnetic resonance (NMR) relaxometry, and the microstructure was systematically investigated by using mercury intrusion porosimetry (MIP), back-scattered electron (BSE) image and energy-dispersive X-ray spectroscopy (EDX) analysis. Results showed that the SAPs in the cement paste containing accelerators had various influences on setting time and compressive strength depending on the type of accelerators. The presence of SAPs in the cement paste containing alkaline free accelerators could alleviate the decrease of internal relative humidity, promote hydration and help to modify the pore structure. Moreover, it was observed that the SAP cavities could be nicely filled with calcium hydroxide (CH) in the cement paste with alkaline free accelerators.
Study of Structure and Mechanical Properties of Fine-Grained Aluminum Alloys Al-0.6wt.%Mg-Zr-Sc with Ratio Zr:Sc = 1.5 Obtained by Cold Drawing
Authors: Nokhrin Aleksey , Shadrina Iana , Chuvil’deev Vladimir , Kopylov Vladimir
Published: January 2019

The thermal stability of a fine-grained (FG) aluminum wire has been studied in Al-0.6Mg-Zr-Sc alloys with various scandium and zirconium contents. Specimens were obtained by induction casting followed by cold deformation. The FG alloys have been demonstrated to have high thermal stability of the structure and properties due to the annealing pretreatment (320 °C, 2 h, before drawing), which results in deposition of Al3(ScxZr1−x) intermetallic particles. It has been determined that following a prolonged annealing treatment (400 °C, 100 h), the alloys retain a uniform fine-grained structure with an average grain size of 2.4–2.8 μm whereas their microhardness measures 405–440 MPa.
Effect of Ti Addition on the Microstructure and Mechanical Properties of SiC Matrix Composites Infiltrated by Al–Si (10 wt.%)–xTi Alloy
Authors: Yu Yajun , Du An , Zhao Xue , Fan Yongzhe , Ma Ruina , Li Shijie , Wang Wei , Cui Yaqi , Cao Xiaoming
Published: January 2019

This paper proposes a simple reactive melt infiltration process to improve the mechanical properties of silicon carbide (SiC) ceramics. SiC matrix composites were infiltrated by Al–Si (10 wt.%)–xTi melts at 900 °C for 4 h. The effects of Ti addition on the microstructure and mechanical properties of the composites were investigated. The results showed that the three-point bending strength, fracture toughness (by single-edge notched beam test), and fracture toughness (by Vickers indentation method) of the SiC ceramics increased most by 34.3%, 48.5%, and 128.5%, respectively, following an infiltration with the Al–Si (10 wt.%)–Ti (15 wt.%) melt. A distinct white reaction layer mainly containing a Ti3Si(Al)C2 phase was formed on the surface of the composites infiltrated by Al alloys containing Ti. Ti–Al intermetallic compounds were scattered in the inner regions of the composites. With the increase in the Ti content (from 0 to 15 wt.%) in the Al alloy, the relative contents of Ti3Si(Al)C2 and Ti–Al intermetallic compounds increased. Compared with the fabricated composite infiltrated by an Al alloy without Ti, the fabricated composites infiltrated by Al alloys containing Ti showed improved overall mechanical properties owing to formation of higher relative content Ti3Si(Al)C2 phase and small amounts of Ti–Al intermetallic compounds.
The Influence of Copper Substrates on Irradiation Effects of Graphene: A Molecular Dynamics Study
Authors: Wang Shulong , Zhang Qian , Yin Kai , Gao Bo , Zhang Siyu , Wang Guoping , Liu Hongxia
Published: January 2019

In this paper, classical molecular dynamics simulations are conducted to study the graphene grown on copper substrates under ion beam irradiation, in which the emphasis is put on the influence copper substrate on a single graphene layer. It can be inferred that the actual transmission and distribution of kinetic energy from incident ion play important roles in irradiation-defects forming process together. The minimum value needed to generate defects in supported graphene is higher than 2.67 keV, which is almost twice the damage threshold as the suspended graphene sheet. This work indicates the presence of copper substrate increases the damage threshold of graphene. Additionally, our results provide an atomistic explanation for the graphene with copper substrate under ion irradiation, which is very important for engineering graphene.
Autogenous Shrinkage, Microstructure, and Strength of Ultra-High Performance Concrete Incorporating Carbon Nanofibers
Authors: Lim Jacob L. G. , Raman Sudharshan N. , Safiuddin Md. , Zain Muhammad Fauzi Mohd. , Hamid Roszilah
Published: January 2019

The mix design of ultra-high performance concrete (UHPC) is complicated by the presence of many “ingredients.” The fundamental packing density allows a simpler mix design with fewer ingredients to achieve optimum packing density and dense microstructure. The optimum particle grading increases the flowability of UHPC and eliminates entrapped air. This study presents a simplified particle grading design approach that positively influences the strength, autogenous shrinkage, and microstructure characteristics of UHPC. Carbon nanofibers (CNFs) of superior mechanical properties were added to enhance the strength of UHPC and to reduce its autogenous shrinkage. In addition, ground granulated blast-furnace slag (GGBS) was used as a cement replacement material to reduce the amount of cement in UHPC mixes. Test results showed that the presence of homogeneously dispersed CNF increased the compressive strength and compensated the autogenous shrinkage of UHPC. The findings indicated that an ideal particle distribution, which is close to the modified Andreasen and Andersen grading model, contributed to achieving high compressive strength and CNFs were capable of providing nano-bridges to compensate the shrinkage caused by GGBS.