bluuebunny/crossref_metadata_2025 huggingface dataset:
Preserving Farmland on the Urban Fringe: A Literature Review on Land Policies in Developed Countries
Authors: Perrin Coline , Clément Camille , Melot Romain , Nougarèdes Brigitte
Published: July 2020

This paper reviews the recent literature dealing with farmland protection (FP) policies in developed countries from a planning perspective, with a specific focus on the Mediterranean region. It provides coverage of French language papers that may have been omitted in previous reviews. While the Mediterranean is often pointed out as a region with acute challenges related to food security and a lack of effective planning policies, the literature underlines that issues related to FP policies are similar across the world. Hence, this review may bring valuable insights for more sustainable management of farmland on the urban fringe. It maps several interesting areas of research concerning the often implicit and disparate rationales of FP policies as well as the barriers and potential avenues for improvement for FP. It highlights that FP cannot rely merely on transferring policy tools that have proven successful elsewhere. It also reveals that land policies do not always take into account the specific needs of farming systems, as they often focus on land rather than on agriculture. Further research is thus needed to reveal the interaction over time between the use of certain FP tools and the unique local features of urban fringe agriculture. This review may be of interest to students and scholars, but also to practitioners, policy makers and local groups looking for innovative, more flexible or locally suited farmland protection programs.
Optimization of Molecular Dynamics Simulations of c-MYC1-88—An Intrinsically Disordered System
Authors: Sullivan Sandra S. , Weinzierl Robert O.J.
Published: July 2020

Many of the proteins involved in key cellular regulatory events contain extensive intrinsically disordered regions that are not readily amenable to conventional structure/function dissection. The oncoprotein c-MYC plays a key role in controlling cell proliferation and apoptosis and more than 70% of the primary sequence is disordered. Computational approaches that shed light on the range of secondary and tertiary structural conformations therefore provide the only realistic chance to study such proteins. Here, we describe the results of several tests of force fields and water models employed in molecular dynamics simulations for the N-terminal 88 amino acids of c-MYC. Comparisons of the simulation data with experimental secondary structure assignments obtained by NMR establish a particular implicit solvation approach as highly congruent. The results provide insights into the structural dynamics of c-MYC1-88, which will be useful for guiding future experimental approaches. The protocols for trajectory analysis described here will be applicable for the analysis of a variety of computational simulations of intrinsically disordered proteins.
Experimental Study on One-Way BFRP Bar-Reinforced UHPC Slabs under Concentrated Load
Authors: Xu Xiaoqing , Hou Zhujian
Published: July 2020

The application of fiber-reinforced polymer (FRP) bars and ultra-high performance concrete (UHPC) in the field of civil engineering is promising. An innovative FRP bar-reinforced UHPC short-ribbed bridge deck slab, with low self-weight and high structural performance, was proposed in this study. The behavior of one-way basalt FRP (BFRP) bar-reinforced UHPC slabs under concentrated load was experimentally investigated, and compared with that of a steel bar-reinforced UHPC slab. The ultimate capacity of the one-way BFRP bar-reinforced UHPC slab was 0.59 times that of the steel bar-reinforced UHPC slab, while its ductility was better. Increasing the reinforcement ratio and loading area was beneficial to the ductility of one-way BFRP bar-reinforced UHPC slabs. Moreover, the model proposed by EI-Gamal et al. was found to be suitable for evaluating the punching shear capacities of one-way BFRP bar-reinforced UHPC slabs. However, the model failed to consider the unique strain-hardening characteristics of UHPC, which led to conservative prediction.
The Influence of the Aircraft Operating Fluids on the Mechanical Parameters of the Airport Surface Concrete
Authors: Żebrowski Wojciech , Wolka Paweł , Kurpinska Marzena
Published: July 2020

The authors of the article assessed the impact of operating fluids used to service aircraft on changing mechanical parameters of cement concrete intended for airport pavement. The research concerned concrete designed with the use of CEM I 42.5N LH NA low-alkali cement, broken granite aggregate, fine washed aggregate, and admixtures. The analysis included the assessment of changes in differences in endurance parameters over various research periods of up to 140 days. The obtained results allowed to carry out statistical analysis using the student’s T-test. Research has shown a significant impact of operational fluids used in aircraft on the surface concrete properties of the airport. A reduction in the compressive strength of concrete exposed to one of the tested operating liquid to a reduction of 7.2% was observed over a period of 140 days, while there was no significant impact of operating fluids on tensile strength at splitting.
Feasibility Evaluation of Local Laser Treatment for Strengthening of Thin-Walled Structures from Low-Carbon Steel Subjected to Bending
Authors: Kapustynskyi Oleksandr , Višniakov Nikolaj , Zabulionis Darius , Piščalov Artur
Published: July 2020

This paper is devoted to investigating numerically, by finite element analysis (FEA), and analytically the influences and effects of laser processing of the surface of thin-plate, low-carbon structural steel. The plate mechanical properties—axial and flexural stiffnesses, force-deflection behavior and cross-section force-strain behavior—are investigated after different laser treatments. An analytical methodology of the estimation of the cross-section area of the laser-processed metal is also proposed in the present article, that can be applied to choosing the reasonable distance between the centers of the laser-processed tracks. The methodology takes into account the width of the laser-processed tracks and the distances between these tracks. The experimental, finite element numerical and analytical analyses showed that the laser treatments of the surface of the steel plate increase the yield point of the laser-processed metal and the axial and flexural stiffnesses of the plate.
Simultaneous Analysis of Paracetamol and Diclofenac Using MWCNTs-COOH Modified Screen-Printed Carbon Electrode and Pulsed Potential Accumulation
Authors: Sasal Agnieszka , Tyszczuk-Rotko Katarzyna , Wójciak Magdalena , Sowa Ireneusz , Kuryło Michał
Published: July 2020

A differential-pulse adsorptive stripping voltammetric (DPAdSV) procedure with the use of pulsed potential accumulation and carboxyl functionalized multiwalled carbon nanotubes modified screen-printed carbon electrode (SPCE/MWCNTs-COOH) was delineated for simultaneous analysis of paracetamol (PA) and diclofenac (DF). The use of carboxyl functionalized MWCNTs and pulsed potential accumulation improves the analytical signals of PA and DF, and minimizes interferences from surfactants. After optimization of analytical conditions for this sensor, the peak currents of the two compounds were found to increase linearly with the increase in their concentration (5.0 × 10−9–5.0 × 10−6 mol L−1 with a detection limit of 1.4 × 10−9 mol L−1 for PA, and 1.0 × 10−10–2.0 × 10−8 mol L−1 with a detection limit of 3.0 × 10−11 mol L−1 for DF). For the first time, the electrochemical sensor allows simultaneous determination of PA and DF at concentrations of 24.3 ± 0.5 nmol L−1 and 3.7 ± 0.7 nmol L−1, respectively, in wastewater samples purified in a sewage treatment plant.
Construction of Solitary Two-Wave Solutions for a New Two-Mode Version of the Zakharov-Kuznetsov Equation
Authors: Jaradat Imad , Alquran Marwan
Published: July 2020

A new two-mode version of the generalized Zakharov-Kuznetsov equation is derived using Korsunsky’s method. This dynamical model describes the propagation of two-wave solitons moving simultaneously in the same direction with mutual interaction that depends on an embedded phase-velocity parameter. Three different methods are used to obtain exact bell-shaped soliton solutions and singular soliton solutions to the proposed model. Two-dimensional and three-dimensional plots are also provided to illustrate the interaction dynamics of the obtained two-wave exact solutions upon increasing the phase-velocity parameter.
The Size-Dependent Thermoelastic Vibrations of Nanobeams Subjected to Harmonic Excitation and Rectified Sine Wave Heating
Authors: Abouelregal Ahmed E. , Marin Marin
Published: July 2020

In this article, a nonlocal thermoelastic model that illustrates the vibrations of nanobeams is introduced. Based on the nonlocal elasticity theory proposed by Eringen and generalized thermoelasticity, the equations that govern the nonlocal nanobeams are derived. The structure of the nanobeam is under a harmonic external force and temperature change in the form of rectified sine wave heating. The nonlocal model includes the nonlocal parameter (length-scale) that can have the effect of the small-scale. Utilizing the technique of Laplace transform, the analytical expressions for the studied fields are reached. The effects of angular frequency and nonlocal parameters, as well as the external excitation on the response of the nanobeam are carefully examined. It is found that length-scale and external force have significant effects on the variation of the distributions of the physical variables. Some of the obtained numerical results are compared with the known literature, in which they are well proven. It is hoped that the obtained results will be valuable in micro/nano electro-mechanical systems, especially in the manufacture and design of actuators and electro-elastic sensors.
The Influences of Asymmetric Market Information on the Dynamics of Duopoly Game
Authors: Askar Sameh S.
Published: July 2020

We investigate the complex dynamic characteristics of a duopoly game whose players adopt a gradient-based mechanism to update their outputs and one of them possesses in some way certain information about his/her opponent. We show that knowing such asymmetric information does not give any advantages but affects the stability of the game’s equilibrium points. Theoretically, we prove that the equilibrium points can be destabilized through Neimark-Sacker followed by flip bifurcation. Numerically, we prove that the map describing the game is noninvertible and gives rise to several stable attractors (multistability). Furthermore, the dynamics of the map give different shapes of quite complicated attraction basins of periodic cycles.
Neck Dissection for Cervical Lymph Node Metastases from Remote Primary Malignancies
Authors: Shinohara Shogo , Harada Hiroyuki , Kikuchi Masahiro , Takebayashi Shinji , Hamaguchi Kiyomi
Published: July 2020

Background and Objectives: Patients with cervical lymph node metastases from remote primary tumours have poor prognoses because of the advanced stage of their cancer. Owing to recent progress in the nonsurgical management of various cancer types, options for surgical treatment to reduce tumour volume are increasing, and may help improve survival rates. For example, neck dissection may be a good option as a definitive therapy for some patients with resectable cervical metastases. We assessed patients who underwent neck dissection with curative intent and discuss the effectiveness of this approach for cervical metastases from remote malignancies. Material and Methods: We retrospectively reviewed the data of 18 patients (10 males and 8 females in an age range of 30–79 years) who underwent neck dissections for neck lymph node metastases from a remote primary tumour between 2010 and 2019. Patient clinical characteristics, preoperative accuracy of positive node localisation using fluorodeoxyglucose positron emission tomography–computed tomography (FDG/PET-CT), and patient survival rates were estimated. Results: Primary sites included ten lungs, two mammary glands, one thymus, one thoracic oesophagus, one stomach, one uterine cervix, one ovary, and one testis per patient. There were 19 levels with FDG/PET-CT positive nodes in 17 out of 18 patients. Conversely, there were 28 pathological positive levels out of 50 dissected levels. The sensitivity, specificity, positive and negative predictive values, and accuracy of FDG-PET/CT in predicting positive nodes were 69%, 88%, 95%, 47%, and 74%, respectively. The three-year overall survival (OS) rate for all patients was 70%. The three-year OS rate of the group with zero or one pathological positive nodes was 81%, which was significantly higher than that of the group with more than two positive nodes (51%) (p = 0.03). Conclusions: Neck dissection for cervical lymph node metastases from remote primary malignancies may improve prognoses, especially considering anticancer agents and radiotherapy advancements.
In Situ Raman Characterization of SOFC Materials in Operational Conditions: A Doped Ceria Study
Authors: Solís Cecilia , Balaguer María , Serra José M.
Published: July 2020

The particular operational conditions of electrochemical cells make the simultaneous characterization of both structural and transport properties challenging. The rapidity and flexibility of the acquisition of Raman spectra places this technique as a good candidate to measure operating properties and changes. Raman spectroscopy has been applied to well-known lanthanide ceria materials and the structural dependence on the dopant has been extracted. The evolution of Pr-doped ceria with temperature has been recorded by means of a commercial cell showing a clear increment in oxygen vacancies concentration. To elucidate the changes undergone by the electrolyte or membrane material in cell operation, the detailed construction of a homemade Raman cell is reported. The cell can be electrified, sealed and different gases can be fed into the cell chambers, so that the material behavior in the reaction surface and species evolved can be tracked. The results show that the Raman technique is a feasible and rather simple experimental option for operating characterization of solid-state electrochemical cell materials, although the treatment of the extracted data is not straightforward.
Can We Trust Score Plots?
Authors: Bevilacqua Marta , Bro Rasmus
Published: July 2020

In this paper, we discuss the validity of using score plots of component models such as partial least squares regression, especially when these models are used for building classification models, and models derived from partial least squares regression for discriminant analysis (PLS-DA). Using examples and simulations, it is shown that the currently accepted practice of showing score plots from calibration models may give misleading interpretations. It is suggested and shown that the problem can be solved by replacing the currently used calibrated score plots with cross-validated score plots.
Changes in Metabolites During an Oral Glucose Tolerance Test in Early and Mid-Pregnancy: Findings from the PEARLS Randomized, Controlled Lifestyle Trial
Authors: Haslam Danielle E. , Li Jun , Liang Liming , Martinez Marijulie , Palacios Cristina , Trak-Fellermeier Maria A. , Franks Paul W. , Joshipura Kaumudi , Bhupathiraju Shilpa N.
Published: July 2020

The oral glucose tolerance test (OGTT) is used to diagnose gestational and other types of diabetes. We examined metabolite changes during an OGTT, and how a comprehensive diet and physical activity intervention may influence these changes in a population of overweight/obese Hispanic pregnant women. Integration of changes in metabolites during an OGTT may help us gain preliminary insights into how glucose metabolism changes during pregnancy. Among women from the Pregnancy and EARly Lifestyle improvement Study (PEARLS), we measured metabolites during a multipoint OGTT (fasting, 30, 60 and 120 min) at early and mid-pregnancy. Metabolite levels were measured by liquid chromatography–mass spectrometry in plasma samples in the lifestyle intervention (n = 13) and control (n = 16) arms of the study. A total of 65 candidate metabolites were selected that displayed changes during an OGTT in previous studies. Paired and unpaired t-tests were used to examine differences in Δfast-120 min: (1) at early and mid-pregnancy; and (2) by intervention assignment. We applied principal component analysis (PCA) to identify those metabolites that differed by intervention assignment and OGTT time points. Most of the characteristic changes in metabolites post-OGTT were similar at both gestational time points. PCA identified characteristic metabolite patterns associated with OGTT time points at both early and mid-pregnancy. These metabolites included ketone bodies, tryptophan, acyl carnitines, polyunsaturated fatty acids, and biomarkers related to bile acid, urea cycle, arginine, and proline metabolism. PCA identified distinct Δfast-120 min in fatty acid, acyl carnitine, bile acid, ketone body, and amino acid levels at mid- compared to early pregnancy. Participants in the intervention group did not display mean decreases in Δfast-120 min of several long-chain acyl carnitines that were observed in the control group. These findings provide preliminary insight into metabolites, whose role in increased insulin resistance during pregnancy, should be explored further in future studies.
Optofluidic Formaldehyde Sensing: Towards On-Chip Integration
Authors: Mariuta Daniel , Govindaraji Arumugam , Colin Stéphane , Barrot Christine , Le Calvé Stéphane , Korvink Jan G. , Baldas Lucien , Brandner Jürgen J.
Published: July 2020

Formaldehyde (HCHO), a chemical compound used in the fabrication process of a broad range of household products, is present indoors as an airborne pollutant due to its high volatility caused by its low boiling point ( T = − 19 °C). Miniaturization of analytical systems towards palm-held devices has the potential to provide more efficient and more sensitive tools for real-time monitoring of this hazardous air pollutant. This work presents the initial steps and results of the prototyping process towards on-chip integration of HCHO sensing, based on the Hantzsch reaction coupled to the fluorescence optical sensing methodology. This challenge was divided into two individually addressed problems: (1) efficient airborne HCHO trapping into a microfluidic context and (2) 3,5–diacetyl-1,4-dihydrolutidine (DDL) molecular sensing in low interrogation volumes. Part (2) was addressed in this paper by proposing, fabricating, and testing a fluorescence detection system based on an ultra-low light Complementary metal-oxide-semiconductor (CMOS) image sensor. Two three-layer fluidic cell configurations (quartz–SU-8–quartz and silicon–SU-8–quartz) were tested, with both possessing a 3.5 µL interrogation volume. Finally, the CMOS-based fluorescence system proved the capability to detect an initial 10 µg/L formaldehyde concentration fully derivatized into DDL for both the quartz and silicon fluidic cells, but with a higher signal-to-noise ratio (SNR) for the silicon fluidic cell ( S N R s i l i c o n = 6.1 ) when compared to the quartz fluidic cell ( S N R q u a r t z = 4.9 ). The signal intensity enhancement in the silicon fluidic cell was mainly due to the silicon absorption coefficient at the excitation wavelength,   a ( λ a b s = 420   nm ) = 5 × 10 4   cm − 1 , which is approximately five times higher than the absorption coefficient at the fluorescence emission wavelength, a ( λ e m = 515   nm ) = 9.25 × 10 3   cm − 1 .
Bio- and Nanotechnology as the Key for Clinical Application of Salivary Peptide Histatin: A Necessary Advance
Authors: Reis Zambom Carolina , Henrique da Fonseca Fauller , Santesso Garrido Saulo
Published: July 2020

Candida albicans is a common microorganism of human’s microbiota and can be easily found in both respiratory and gastrointestinal tracts as well as in the genitourinary tract. Approximately 30% of people will be infected by C. albicans during their lifetime. Due to its easy adaptation, this microorganism started to present high resistance to antifungal agents which is associated with their indiscriminate use. There are several reports of adaptive mechanisms that this species can present. Some of them are intrinsic alteration in drug targets, secretion of extracellular enzymes to promote host protein degradation and efflux receptors that lead to a diminished action of common antifungal and host’s innate immune response. The current review aims to bring promising alternatives for the treatment of candidiasis caused mainly by C. albicans. One of these alternatives is the use of antifungal peptides (AFPs) from the Histatin family, like histatin-5. Besides that, our focus is to show how nanotechnology can allow the application of these peptides for treatment of this microorganism. In addition, our intention is to show the importance of nanoparticles (NPs) for this purpose, which may be essential in the near future.
Low-Fat and High-Quality Fermented Sausages
Authors: Tremonte Patrizio , Pannella Gianfranco , Lombardi Silvia Jane , Iorizzo Massimo , Vergalito Franca , Cozzolino Autilia , Maiuro Lucia , Succi Mariantonietta , Sorrentino Elena , Coppola Raffaele
Published: July 2020

The present study, considering for the first time microbiological concerns due to the use of lemon albedo as a fat replacer, aimed at the selection of an anti-Listeria strain to be used as protective culture in low-fat southern Italian fermented sausages. In fact, these kinds of products require appropriate bio-protective strategies to avoid risks due to Listeria monocytogenes. Sixty-seven Lactiplantibacillus plantarum strains isolated from diverse sources were screened for their antimicrobial activity and their interaction with starter strains (Latilactobacillus sakei 152 and Staphylococcus xylosus MVS9). Lactiplantibacillus plantarum Lpls100, highlighting both listericidal activity and the ability to promote Staphylococcus xylosus MVS9 growth, was used as a protective strain in low-fat fermented sausages prepared with lemon albedo as a fat replacer. The effect of the albedo and the protective strain on the fermentation process and the final quality was ascertained. Results highlighted that the use of the albedo did not affect the growth of starter strains and enhanced some quality features, such as fatty acid profiles and certain sensory attributes. However, the albedo also produced a slow decrease in water activity, compromising the microbial quality. The anti-Listeria strain, enhancing coagulase negative cocci growth and exerting antimicrobial activity, avoided the inconveniences caused by the use of the albedo. Moreover, the anti-Listeria effectiveness was assessed through a challenge test using a Listeria cocktail. The study revealed that Lactiplantibacillus plantarum Lpls100, regardless of the presence of the albedo, assures a prompt inhibition of Listeria spp. Therefore, its use could be an important contribution to the quality of low-fat fermented sausages.
Zircon U-Pb Dating and Petrogenesis of Multiple Episodes of Anatexis in the North Dabie Complex Zone, Central China
Authors: Yang Yang , Liu Yi-Can , Li Yang , Groppo Chiara , Rolfo Franco
Published: July 2020

The North Dabie complex zone (NDZ), central China, is a high-T ultrahigh-pressure (UHP) metamorphic terrane. It underwent a complex evolution comprising of multistage metamorphism and multiple anatectic events during the Mesozoic continental collision, characterized by granulite-facies overprinting and a variety of migmatites with different generations of leucosomes. In this contribution, we carried out an integrated study including field investigation, petrographic observations, zircon U-Pb dating, and whole-rock element and Sr-Nd-Pb isotope analysis for the migmatites in the NDZ and their leucosomes and melanosomes. As a result, four groups of leucosomes have been recognized: Group 1 (garnet-bearing leucosome), strongly deformed leucosomes with coarse-grained peritectic garnet; Group 2 (amphibole-rich leucosome), weakly deformed to undeformed amphibole-rich leucosomes with coarse-grained peritectic amphibole and no garnet; Group 3 (amphibole-poor leucosome), weakly deformed to undeformed amphibole-poor leucosomes with minor fine-grained amphibole; Group 4 (K-feldspar-rich leucosome), K-feldspar-rich leucosomes mainly composed of coarse-grained quartz, plagioclase and K-feldspar. Zircon SHRIMP and LA-ICPMS U-Pb dating suggest that the Group 1 leucosomes formed at 209 ± 2 Ma whereas the rest of the leucosome groups (Groups 2–4) occurred between 145–110 Ma, in response to decompression under granulite-facies conditions during the early stage of exhumation, and to heating during post-orogenic collapse, respectively. Furthermore, the garnet-bearing leucosomes were resulted from fluid-absent anatexis related to biotite dehydration melting, while the other three groups of leucosomes were formed during large-scale fluid-present partial melting and coeval migmatization. This migmatization comes from heating from the mountain-root removal and asthenosphere upwelling, together with the influx of fluids derived from country rocks at mid-upper crustal levels. However, all the leucosomes and melanosomes display Pb-isotopic compositions similar to those observed for the NDZ UHP rocks (eclogites and granitic gneisses), suggesting a common source from the Triassic subducted Neoproterozoic lower-crustal rocks. In addition, the Cretaceous partial melting and migmatization began at 143 ± 2 Ma with three age-peaks at 133 ± 3 Ma, 124 ± 3 Ma and 114 ± 7 Ma, respectively.
An Improvement on Selective Separation by Applying Ultrasound to Rougher and Re-Cleaner Stages of Copper Flotation
Authors: Hassanzadeh Ahmad , Sajjady Sayed Ali , Gholami Hamed , Amini Saeed , Özkan Safak Gökhan
Published: July 2020

It has been known that the power ultrasound is used as a pretreatment and rarely applied as a simultaneous method to improve grade and recovery during froth flotation processes. This work aimed at investigating the impact of simultaneously used ultrasonic waves under variant operating configurations on the flotation of representative porphyry copper ore during rougher and re-cleaner stages. For this purpose, four different operating outlines were examined as (I) conventional flotation, (II) homogenizer, (III) ultrasonic bath, and (IV) combination of a homogenizer and an ultrasonic bath. The ultrasonic vibration was generated by the homogenizer (21 kHz, 1 kW) in the froth zone and ultrasonic bath (35 kHz, 0.3 kW) in the bulk zone. The rougher and re-cleaner flotation experiments were conducted using Denver-type mechanically agitated cells with 4.2 and 1 L capacities, respectively. The results showed that using the homogenizer (at 0.4 kW) slightly affected the selectivity separation index of chalcopyrite and pyrite, although it positively increased the grade of chalcopyrite from 21.5% to 25.7%. The ultrasonic-assisted flotation experiments with the ultrasonic bath and its combination with the homogenizer (0.4 kW) (i.e., configurations III and IV) led to an increase of approximately 16.1% and 26.9% in the chalcopyrite selectivity index compared to the conventional flotation, respectively. At the cleaning stage, a lower grade of aluminum silicate-based minerals was obtained desirably in every ultrasonic-treated configuration, which was supported with the water recoveries. Finally, applying the homogenizer and its combination with the ultrasonic bath were recommended for re-cleaner and rougher stages, respectively. Further fundamental and practical knowledge gaps required to be studied were highlighted.
Design, Synthesis and Biological Evaluation of Novel Anthraniloyl-AMP Mimics as PQS Biosynthesis Inhibitors Against Pseudomonas aeruginosa Resistance
Authors: Sabir Shekh , Subramoni Sujatha , Das Theerthankar , Black David StC. , Rice Scott A. , Kumar Naresh
Published: July 2020

The Pseudomonas quinolone system (PQS) is one of the three major interconnected quorum sensing signaling systems in Pseudomonas aeruginosa. The virulence factors PQS and HHQ activate the transcription regulator PqsR (MvfR), which controls several activities in bacteria, including biofilm formation and upregulation of PQS biosynthesis. The enzyme anthraniloyl-CoA synthetase (PqsA) catalyzes the first and critical step in the biosynthesis of quinolones; therefore, it is an attractive target for the development of anti-virulence therapeutics against Pseudomonas resistance. Herein, we report the design and synthesis of novel triazole nucleoside-based anthraniloyl- adenosine monophosphate (AMP) mimics. These analogues had a major impact on the morphology of bacterial biofilms and caused significant reduction in bacterial aggregation and population density. However, the compounds showed only limited inhibition of PQS and did not exhibit any effect on pyocyanin production.
Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages
Authors: Kim Nam-Hun , Kim Hong-Ki , Lee Ji-Hak , Jo Seung-Il , Won Hye-Min , Lee Gyeong-Seok , Lee Hyoun-Su , Nam Kung-Woo , Kim Wan-Jong , Han Man-Deuk
Published: July 2020

The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome has been implicated in a variety of diseases, including atherosclerosis, neurodegenerative diseases, and infectious diseases. Thus, inhibitors of NLRP3 inflammasome have emerged as promising approaches to treat inflammation-related diseases. The aim of this study was to explore the effects of juglone (5-hydroxyl-1,4-naphthoquinone) on NLRP3 inflammasome activation. The inhibitory effects of juglone on nitric oxide (NO) production were assessed in lipopolysaccharide (LPS)-stimulated J774.1 cells by Griess assay, while its effects on reactive oxygen species (ROS) and NLRP3 ATPase activity were assessed. The expression levels of NLRP3, caspase-1, and pro-inflammatory cytokines (IL-1β, IL-18) and cytotoxicity of juglone in J774.1 cells were also determined. Juglone was non-toxic in J774.1 cells when used at 10 μM (p < 0.01). Juglone treatment inhibited the production of ROS and NO. The levels of NLRP3 and cleaved caspase-1, as well as the secretion of IL-1β and IL-18, were decreased by treatment with juglone in a concentration-dependent manner. Juglone also inhibited the ATPase activities of NLRP3 in LPS/ATP-stimulated J774.1 macrophages. Our results suggested that juglone could inhibit inflammatory cytokine production and NLRP3 inflammasome activation in macrophages, and should be considered as a therapeutic strategy for inflammation-related diseases.
Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta
Authors: Barrientos Ruth E. , Simirgiotis Mario J. , Palacios Javier , Paredes Adrián , Bórquez Jorge , Bravo Alejandra , Cifuentes Fredi
Published: July 2020

Heliotropium taltalense is an endemic species of the northern coast of Chile and is used as folk medicine. The polyphenolic composition of the methanolic and aqueous extract of the endemic Chilean species was investigated using Ultrahigh-Performance Liquid Chromatography, Heated Electrospray Ionization and Mass Spectrometry (UHPLC-Orbitrap-HESI-MS). Fifty-three compounds were detected, mainly derivatives of benzoic acid, flavonoids, and some phenolic acids. Furthermore, five major compounds were isolated by column chromatography from the extract, including four flavonoids and one geranyl benzoic acid derivative, which showed vascular relaxation and were in part responsible for the activity of the extracts. Since aqueous extract of H. taltalense (83% ± 9%, 100 μg/mL) produced vascular relaxation through an endothelium-dependent mechanism in rat aorta, and the compounds rhamnocitrin (89% ± 7%; 10−4 M) and sakuranetin (80% ± 6%; 10−4 M) also caused vascular relaxation similar to the extracts of H. taltalense, these pure compounds are, to some extent, responsible for the vascular relaxation.
Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1
Authors: Vanti Giulia , Ntallis Sotirios G. , Panagiotidis Christos A. , Dourdouni Virginia , Patsoura Christina , Bergonzi Maria Camilla , Lazari Diamanto , Bilia Anna Rita
Published: July 2020

Essential oils are complex mixtures of strongly active compounds, very volatile and sensitive to light, oxygen, moisture and temperature. Loading inside nanocarriers can be a strategy to increase their stability and successfully use them in therapy. In the present study, a commercial Melissa officinalis L. (Lamiaceae) essential oil (MEO) was analyzed by gas chromatography-mass spectrometry, loaded inside glycerosomes (MEO-GS) and evaluated for its anti-herpetic activity against HSV type 1. MEO-GS analyses were prepared by the thin layer evaporation method and they were characterized by light scattering techniques, determining average diameter, polydispersity index and ζ-potential. By transmission electron microscopy, MEO-GS appeared as small nano-sized vesicles with a spherical shape. MEO encapsulation efficiency inside glycerosomes, in terms of citral and β-caryophyllene, was found to be ca. 63% and 76% respectively, and MEO release from glycerosomes, performed by dialysis bag method, resulted in less than 10% within 24h. In addition, MEO-GS had high chemical and physical stability during 4 months of storage. Finally, MEO-GS were very active in inhibiting HSV type 1 infection of mammalian cells in vitro, without producing cytotoxic effects. Thus, MEO-GS could be a promising tool in order to provide a suitable anti-herpetic formulation.
Method Validation and Investigation of the Levels of Pharmaceuticals and Personal Care Products in Sludge of Wastewater Treatment Plants and Soils of Irrigated Golf Course
Authors: Ademoyegun Olufemi Temitope , Okoh Omobola Oluranti , Okoh Anthony Ifeanyi
Published: July 2020

The validation of a sensitive and reliable analytical procedure for the determination of pharmaceutical and personal care products (PPCPs) in solid environmental samples is reported in this study. Initially, two types of derivatization were used for the identification of the 13 target PPCP standards (acylation and silylation), but silylation proved to be better in sensitivity as it detected all of the analytes under investigation. Samples were extracted using an ultrasonicator, concentrated and re-dissolved in 100 mL water, then cleaned-up using C18 cartridges before silylation that preceded the Gas chromatography-mass Spectrometry detector (GC–MS) analyses. The optimized method provided a linear response over the range of 10–400 ng·g−1 with r2 > 0.992 and satisfactory recoveries (>45.6%) for the 13 compounds of interest. In this study, the variation of the sonication temperature, type of organic solvent for extraction, and types of cartridge were used to optimize the extraction procedure. A good repeatability (within day) and reproducibility (between days) with a relative standard deviation (RSD) that was equal or less than 13% for all the PPCPs were achieved with the developed extraction procedures for the irrigated soil and sewage sludge samples. The limits of detection (LODs) of the tested compounds varied from 0.1 ng·g−1 (aspirin) to 1.4 ng·g−1 (doxycycline) and from 0.1 ng·g−1 (codiene) to 1.7 ng·g−1 (doxycycline) for soils and sewage sludge samples, respectively. The method was successfully applied to the sludge of wastewater treatment plants and soils of an irrigated golf course. Among the tested emerging pollutants, paracetamol showed the highest concentration value of 98.9 ng·g−1 in the sludge, and for the irrigated soil (0 to 10 cm), the concentration ranged from 1.16 ng·g−1 (aspirin) to 8.57 ng·g−1 (ibuprofen).
Anti-Inflammatory and Antioxidant Activities of Terpene- and Polyphenol-Rich Premna odorata Leaves on Alcohol-Inflamed Female Wistar Albino Rat Liver
Authors: Elmaidomy Abeer H. , Alhadrami Hani A. , Amin Elham , Aly Hanan F. , Othman Asmaa M. , Rateb Mostafa E. , Hetta Mona H. , Abdelmohsen Usama Ramadan , M. Hassan Hossam
Published: July 2020

Premna odorata Blanco (Lamiaceae) is an ethnomedicinal plant native to different tropical regions. Although some reports addressed their anti-inflammatory, cytotoxic, and antituberculotic effects, their hepatoprotective potential is yet to be discovered. Accordingly, this study investigated the crude extract and different fractions of the plant leaves; metabolic profiling using liquid chromatography/high-resolution electrospray ionization mass spectroscopy (LC–HRESIMS) analysis, in silico absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties for the dereplicated metabolite via online PreADMET program, ROS scavenger activity on the Hep G2 human liver cancer cell line, and the possible hepatic cellular treatment effects in alcohol-inflamed liver female Wistar albino rats. Metabolic profiling dereplicated a total of 28 metabolites from the crude extract and its various fractions. In silico ADMET and ROS scavenger activity screening suggested plant metabolites are of potential bioactivity. In vivo hepatic treatment with crude, defatted crude, and n-hexane leave extracts suggested all extracts significantly improved liver damage, which was indicated by the reduction of elevated serum levels of bilirubin, AST, ALT, ALP, CRP, TNF-α, ICAM-1, VCAM-1, and MDA. The reduced levels of GSH and TAC were normalized during the study. Histological examinations of liver tissue showed collagen fiber distribution nearly back to its normal pattern. The anti-inflammatory and antioxidant potentials of Premna odorata extracts could be partly related to the combined effects of these phytochemicals or their synergistic interactions.
Biological Activities of Aqueous Extracts from Carob Plant (Ceratonia siliqua L.) by Antioxidant, Analgesic and Proapoptotic Properties Evaluation
Authors: Ben Ayache Siwar , Behija Saafi Emna , Emhemmed Fathi , Flamini Guido , Achour Lotfi , Muller Christian D.
Published: July 2020

The present work describes the volatile compounds profile and phytochemical content of Ceratonia siliqua L. Fifty different components have been identified. Among them, three constituents are shared i.e., 2-methlybutanoic acid, methyl hexanoate and limonene by different common carob preparations: pulp decoction (PD), seeds decoction (SD) and Rob, a sweet syrup extracted from the pulp of the carob pod. Each extract exhibits different volatile aromatic emission profiles. The antioxidant activity of the extracts was evaluated using three methods, DPPH, ABTS and FRAP, producing a dose-dependent response. The IC50, when determined by FRAP, gave the lowest values (0.66 ± 0.01, 0.73 ± 0.05 and 0.55 ± 0.00 mg/mL PD, SD and Rob, respectively). The nociception essay, after intraperitoneal injection of acetic acid in mice, demonstrated that Rob, pulp and seeds decoction extracts showed an efficient inhibition of writhes over time, with persistence over 30 min. The SD decoction revealed the highest efficacy in decreasing the writhing reflex (90.3 ± 1.2%; p < 0.001). Furthermore, the proapoptotic activity of SD against three human cell line, THP-1, MCF-7 and LOVO, evaluated by flow cytometry, showed a significantly stronger proapoptotic activity on colon cancer (LOVO) than on the other cell lines, a phenomenon known as phenotypic selectivity.
Sustainable Carbon as Efficient Support for Metal-Based Nanocatalyst: Applications in Energy Harvesting and Storage
Authors: Buaki-Sogó Mireia , Zubizarreta Leire , García-Pellicer Marta , Quijano-López Alfredo
Published: July 2020

Sustainable activated carbon can be obtained from the pyrolysis/activation of biomass wastes coming from different origins. Carbon obtained in this way shows interesting properties, such as high surface area, electrical conductivity, thermal and chemical stability, and porosity. These characteristics among others, such as a tailored pore size distribution and the possibility of functionalization, lead to an increased use of activated carbons in catalysis. The use of activated carbons from biomass origins is a step forward in the development of more sustainable processes enhancing material recycling and reuse in the frame of a circular economy. In this article, a perspective of different heterogeneous catalysts based on sustainable activated carbon from biomass origins will be analyzed focusing on their properties and catalytic performance for determined energy-related applications. In this way, the article aims to give the reader a scope of the potential of these tailor-made sustainable materials as a support in heterogeneous catalysis and future developments needed to improve catalyst performance. The selected applications are those related with H2 energy and the production of biomethane for energy through CO2 methanation.
7-Acetoxycoumarin Inhibits LPS-Induced Inflammatory Cytokine Synthesis by IκBα Degradation and MAPK Activation in Macrophage Cells
Authors: Park Taejin , Park Jin-Soo , Sim Ji Han , Kim Seung-Young
Published: July 2020

Acetylation involves the chemical introduction of an acetyl group in place of an active hydrogen group into a compound. In this study, we synthesized 7-acetoxycoumarin (7AC) from acetylation of umbelliferone (UMB). We examined the anti-inflammatory properties of 7AC in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. The anti-inflammatory activity of 7AC on viability of treated cells was assessed by measuring the level of expression of NO, PGE2 and pro-inflammatory cytokines, namely interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in 7AC-treated RAW 264.7 macrophages. The 7AC was nontoxic to cells and inhibited the production of cytokines in a concentration-dependent manner. In addition, its treatment suppressed the production of pro-inflammatory cytokines in a dose-dependent manner and concomitantly decreased the protein and mRNA expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, the levels of the phosphorylation of mitogen-activated protein kinase (MAPK) family proteins such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 and nuclear factor kappa B (NF-κB) were reduced by 7AC. In conclusion, we generated an anti-inflammatory compound through acetylation and demonstrated its efficacy in cell-based in vitro assays.
In Vitro Antimycobacterial Activity and Physicochemical Characterization of Diaryl Ether Triclosan Analogues as Potential InhA Reductase Inhibitors
Authors: Ibrahim Tarek S. , Taher Ehab S. , Samir Ebtihal , M. Malebari Azizah , Khayyat Ahdab N. , Mohamed Mamdouh F. A. , Bokhtia Riham M. , AlAwadh Mohammed A. , Seliem Israa A. , Asfour Hani Z. , Alhakamy Nabil A. , Panda Siva S. , AL-Mahmoudy Amany M. M.
Published: July 2020

Two sets of diphenyl ether derivatives incorporating five-membered 1,3,4-oxadiazoles, and their open-chain aryl hydrazone analogs were synthesized in good yields. Most of the synthesized compounds showed promising in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv. Three diphenyl ether derivatives, namely hydrazide 3, oxadiazole 4 and naphthylarylidene 8g exhibited pronounced activity with minimum inhibitory concentrations (MICs) of 0.61, 0.86 and 0.99 μg/mL, respectively compared to triclosan (10 μg/mL) and isoniazid (INH) (0.2 μg/mL). Compounds 3, 4, and 8g showed the InhA reductase enzyme inhibition with higher IC50 values (3.28–4.23 µM) in comparison to triclosan (1.10 µM). Correlation between calculated physicochemical parameters and biological activity has been discussed which justifies a strong correlation with respect to the inhibition of InhA reductase enzyme. Molecular modeling and drug-likeness studies showed good agreement with the obtained biological evaluation. The structural and experimental information concerning these three InhA inhibitors will likely contribute to the lead optimization of new antibiotics for M. tuberculosis.
Chiral Separation and Determination of Etoxazole Enantiomers in Vegetables by Normal-Phase and Reverse-Phase High Performance Liquid Chromatography
Authors: Zhang Ping , He Yuhan , Wang Sheng , Shi Dongmei , Xu Yangyang , Yang Furong , Wang Jianhao , He Lin
Published: July 2020

The chiral separation of etoxazole enantiomers on Lux Cellulose-1, Lux Cellulose-3, Chiralpak IC, and Chiralpak AD chiral columns was carefully investigated by normal-phase high performance liquid chromatography and reverse-phase high performance liquid chromatography (HPLC). Hexane/isopropanol, hexane/n-butanol, methanol/water, and acetonitrile/water were used as mobile phase at a flow rate of 0.8 mL/min. The effects of chiral stationary phase, mobile phase component, mobile phase ratio, and temperature on etoxazole separation were also studied. Etoxazole enantiomers were baseline separated on Lux Cellulose-1, Chiralpak IC, and Chiralpak AD chiral columns, and partially separated on Lux Cellulose-3 chiral column under normal-phase HPLC. However, the complete separation on Lux Cellulose-1, Chiralpak IC, and partial separation on Chiralpak AD were obtained under reverse-phase HPLC. Normal-phase HPLC presented better resolution for etoxazole enantiomers than reverse-phase HPLC. Thermodynamic parameters, including ΔH and ΔS, were also calculated based on column temperature changes from 10 °C to 40 °C, and the maximum resolutions (Rs) were not always acquired at the lowest temperature. Furthermore, the optimized method was successfully applied to determine etoxazole enantiomers in cucumber, cabbage, tomato, and soil. The results of chiral separation efficiency of etoxazole enantiomers under normal-phase and reverse-phase HPLC were compared, and contribute to the comprehensive environmental risk assessment of etoxazole at the enantiomer level.
Influence of Fermentation Water on Stable Isotopic D/H Ratios of Alcohol Obtained from Concentrated Grape Must
Authors: Perini Matteo , Failoni Andrea , Simoni Marco , Tonon Agostino , Camin Federica
Published: July 2020

According to Organisation Internationale de la vigne et du vin (OIV) standards, when analysing the stable isotope ratio of deuterium to hydrogen D/H at the methyl (I) and methylene (II) site of ethanol from concentrated must, a dilution with tap water is needed in order to carry out the alcoholic fermentation. This dilution causes a partial transfer of water hydrogens to the sugar, and this affects the (D/H)I and (D/H)II isotopic values of ethanol, which need to be normalised through specific equations based on the analysis of water δ18O or δ2H. The aim of this study was to evaluate the effectiveness and correctness of these equations experimentally. Grape, cane, and beet sugar, as well as grape must were diluted with water with increasing H and O stable isotope ratios, fermented, and analysed. SNIF-NMR and IRMS techniques were applied following the respective OIV methods. The equations based on the δ2H analysis of the diluted sugar/must solutions proved to be reliable in all the cases, although it is not an OIV standard. When using the equations based on the values of δ18O of the diluted solution, data normalisation was reliable only in cases where the water used for dilution had not undergone isotopic fractionation due, for example, to evaporation. In these cases, δ2H should be analysed.