bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISIS SPASIAL ASPEK KESEHATAN LINGKUNGAN DENGAN KEJADIAN FILARIASIS DI KOTA PEKALONGAN
Authors: Wulandhari Shobiechah Aldillah , Pawenang Eram Tunggul
Published: September 2017

Pekalongan City is one of the endemic cities of filariasis with mf-rate > 1 %. One of many factors that can influence filariasis is environmental factor. The aim of this research was to figure out the spatial imaging of environmental health such as shrub existence, puddle, wastewater pipeline, and livestock existence with filariasis in Pekalongan City. The study was quantitative descriptive with descriptive survey. Data were analyzed with spatial analysis using GIS in 6 filariasis-endemic subdistricts, i.e. Banyurip Ageng, Jenggot, Bandengan, Pabean, Kuripan Lor, dan Kertoharjo. The study showed that filariasis transmission was in the area with shrub and wastewater pipeline existence. Risk factor of wastewater pipeline existence was in all research places. Risk factor of livestock existence was in 3 subdistricts of Bandengan, Kertoharjo, dan Jenggot. Rice fields existence was a environmental risk factor in Kertoharjo and Banyurip Ageng.
ANALISIS KESELAMATAN GEDUNG BARU F5 UNIVERSITAS NEGERI SEMARANG SEBAGAI UPAYA TANGGAP TERHADAP KEADAAN DARURAT
Authors: Widowati Evi , Koesyanto Herry , Wahyuningsih Anik Setyo , Sugiharto Sugiharto
Published: September 2017

Abstrak

 

Gedung F5 adalah gedung baru di Fakultas Ilmu Keolahragaan yang masih memiliki sarana keselamatan gedung yang sangat minim, dimana gedung ini memiliki potensi untuk terjadi kebakaran ataupun bencana alam lainnya. Tujuan penelitian ini adalah menghasilkan suatu rekomendasi sebagai upaya perbaikan dalam aspek keselamatan terhadap gedung baru F5 Universitas Negeri Semarang. Penelitian ini dengan menggunakan metode penelitian deskriptif komparatif. Hasil dari 103 poin yang dibahas, sebanyak 41 poin (39,8%) terpenuhi dan sesuai dengan standar/peraturan. Sebanyak 12 poin (11,7%) terpenuhi namun belum sesuai dengan standar/peraturan dan sebanyak 50 poin (48,5%) tidak terpenuhi. Saran untuk penelitian ini antara lain yaitu memasang instalasi alarm kebakaran, titik panggil manual, hidran, sprinkler, tanda pemasangan pada setiap APAR, dan memasang kembali check sheet yang hilang. Selain itu memasang pintu darurat, tangga darurat, pencahayaan darurat, memperbaiki penunjuk arah evakuasi darurat, menambah 1 area untuk titik berkumpul, menambah 1 akses untuk jalur keluar atau memperlebar jalur akses masuk tersebut menjadi minimal 4 dan ideal 5,5 meter dan melengkapi dengan cermin cembung setiap tikungan yang ada pada akses masuk gedung F5 sebanyak 3 unit.

 

Kata Kunci : Keselamatan, gedung, darurat.

 

 

Abstract

F5 Building is a new building in the Faculty of Sport Science which still has very minimal building safety facilities.The purpose of this research is to produce a recommendation as an effort to improve the safety aspect of the new building of F5 State University of Semarang. This research used comparative descriptive research method and focus on 103 topic points of safety building. From observation and analysis, it resulted 41 points (39.8%) were met and in accordance with the standards / regulations. A total of 12 points (11.7%) are met but not in accordance with the standard / regulation and as many as 50 points (48.5%) are not met. Suggestions for this research include installing fire alarms, manual call points, hydrants, sprinklers, installation marks on each APAR, and reinstalling missing check sheets. In addition to installing emergency exits, emergency stairs, emergency lighting, fixing emergency evacuation directions, adding 1 area for the gathering point, adding 1 access to the exit or widening the access point to a minimum of 4 and ideal 5.5 meters and equipping with a convex mirror any bend that existed on the access entrance of F5 building.

 

Keywords: safety, building, emergency
PENERAPAN MANAJEMEN KESELAMATAN RADIASI DI INSTALASI RADIOLOGI RUMAH SAKIT
Authors: Dianasari Tri , Koesyanto Herry
Published: September 2017

ABSTRAK

Kegiatan radiologi selain dapat memberikan manfaat juga dapat menimbulkan bahaya bagi pekerja radiasi. Untuk mencegah hal tersebut dapat dilakukan dengan menerapkan aspek manajemen keselamatan radiasi. Penelitian ini bertujuan mengetahui gambaran penerapan manajemen keselamatan radiasi pada instalasi radiologi RSUD Ungaran. Jenis penelitian ini menggunakan metode diskriptif kuantitatif dengan teknik pengambilan data observasi dan wawancara. Hasil penelitian ini menunjukkan dari 5 variabel dengan 16 komponen terdiri dari 48 poin. Sebanyak 29 poin (60,42%) terpenuhi dan sesuai dengan standar/ peraturan. Sebanyak 10 poin (20,83%) terpenuhi tetapi belum sesuai dengan standar/peraturan. Sebanyak 9 poin (18,75%) tidak terpenuhi oleh instalasi radiologi RSUD Ungaran.

 

 

ABSTRACT

The radiological activity not only brings benefits but also can damage radiologist. It can be prevented by implementing the aspects of radiation safety management. This study aimed to overview the implementation of radiation safety management in Ungaran Public Hospital radiology instalation. This research used quantitative descriptive method used observations, interviews with three informants, and documentation studies to collect data. The results of this study indicated from 5 variables (16 components consist of 48 ​​points. As much 29 points (60,42%) were fulfilled based on the standards. A total of 10 points (20,83%) were fulfilled but not based on the standards. A total of 9 points (18,75%) were not fulfilled.
PENGENDALIAN KROMIUM (CR) YANG TERDAPAT DI LIMBAH BATIK DENGAN METODE FITOREMEDIASI
Authors: Setiyono Andik , Gustaman Rian Arie
Published: September 2017

Motif dan warna batik dihasilkan dengan pewarna yang mengandung logam berat kromium (Cr). Industri batik secara umum tergolong usaha kecil dan menengah sehingga sebagian besar tidak mengolah limbah batik yang mengandung logam berat Cr. Tujuan penelitian ini adalah memberikan alternatif solusi dari dampak pencemaran Cr dengan memanfaatkan berbagai tumbuhan untuk menyerap Cr pada limbah batik. Penelitian ini adalah kuasi eksperimen dengan rancangan desain pre and post test. Tanaman air yang dipilih adalah enceng gondok (Eichornia crassipes), kayu apu (Pistia stratiotes), dan kayambang (Salvinia cucullata) yang ditanam pada wadah yang berisi air limbah selama 5 hari. Konsentrasi Cr pada air limbah kelompok 1 menurun dari 0,0546 mg/l menjadi 0,0378 mg/l setelah diberi perlakuan dengan enceng gondok. Konsentrasi Cr pada air limbah kelompok 2 menurun dari 0,0488 mg/l menjadi 0,0315 mg/l setelah diberi perlakuan dengan kayambang. Konsentrasi Cr pada air limbah kelompok 1 menurun dari 0,0464 mg/l menjadi 0,0240 mg/l setelah diberi perlakuan dengan kayu apu. Hasil uji statistik dengan uji Kruskal Wallis menunjukkan nilai p= 0,280 sehingga disimpulkan tidak ada perbedaan penyerapan Cr yang terdapat pada limbah batik pada jenis tanaman enceng gondok, kayu apu, dan kayambang.

 

Kata Kunci : Cr, Limbah batik, Fitoremediasi

 

Abstract

Batik motifs and colors are produced with dyes containing heavy metal chromium (Cr). Batik industries is generally classified as small and medium enterprises, so most of them do not process batik waste containing heavy metal Cr. The purpose of this research was to provide alternative solution from Cr pollution impact by utilizing various plants to absorb Cr in batik waste. This research was a quasi experiment with pre and post test design. The selected aquatic plants were water hyacinth or “enceng gondok” (Eichornia crassipes), “kayu apu” (Pistia stratiotes), and “kayambang” (Salvinia cucullata). They were grown in containers containing waste water for 5 days. The Cr concentration in wastewater of group 1 decreased from 0.0546 mg/l to 0.0378 mg/l after being treated with water hyacinth. The Cr concentration in wastewater of group 2 decreased from group 2 from 0.0488 mg/l to 0.0315 mg/l after being treated with “kayambang”. The Cr concentration in wastewater of group 3 decreased from 0.0464 mg/l to 0.0240 mg/l after being treated with “kayu apu”. Statistical test with Kruskal Wallis test showed that p = 0,280. It was concluded that there were no difference in Cr absorbtion among water hyacinth, kayambang and kayu apu.
KORELASI PENGETAHUAN, KEPUASAN, MOTIVASI DENGAN KONSISTENSI PEMAKAIAN KONDOM PADA PELANGGAN WPS DI SUNAN KUNING
Authors: Wirastri Dwi , Deliana Sri Maryati , Mukaromah Siti Baitul
Published: September 2017

Data profil kesehatan kota Semarang tahun 2014 menunjukkan kasus IMS mengalami peningkatan selama 5 tahun terakhir, tahun 2010 sejumlah 2493 dan tahun 2014 sebanyak 5749 kasus. Penggunaan kondom yang rendah mengakibatkan tingginya prevalensi IMS. Tujuan penelitian untuk menganalisis pengetahuan, kepuasan, motivasi dengan konsistensi pemakaian kondom untuk mencegah penularan IMS pada Pelanggan. Metode penelitian ini adalah kuantitatif dengan desain cross sectional. Sampel berjumlah 30 dengan teknik insidental sampling. Hasil uji Chi Square nilai p=0,593 yaitu tidak ada hubungan antara pengetahuan dengan konsistensi pemakain kondom. hasil uji Chi Square nilai p=0,166 yaitu tidak ada hubungan antara kepuasan dengan konsistensi pemakain kondom. uji Chi Square nilai p=0,015 yaitu ada hubungan antara motivasi dengan konsistensi pemakain kondom.  multivariat menunjukan bahwa Motivasi memiliki p< 0,05. Saran yang peneliti rekomendasikan adalah sosialisasi pemberian informasi pentingnya penggunaan kondom pada pelanggan WPS untuk menekan angka kejadian IMS.

 

Kata kunci : pengetahuan, kepuasan, motivasi, konsistensi pemakaian kondom

 

 

 

ABSTRACT

Health profile in Semarang city data in 2014 showed cases of STIs have increased over the last 5 years, in 2010 a number of 2493 and 2014 as many as 5749 cases. Low condom use resulted in a high prevalence of STIs. The aim of research to analyze knowledge, satisfaction, motivation with the consistency of the use of condoms to prevent the transmission of STIs on customers. This research method is quantitative with cross sectional design. Samples numbered 30 with incidental sampling technique. The result of Chi Square test p= 0.593 there is no correlation between knowledge and consistency of condom usage. the results of Chi Square test p-value = 0.166 there is no relationship between satisfaction with the consistency of condom usage. Chi square test p = 0.015 there is a relationship between motivation and consistency of condom usage. Multivariate showed that motivation has a p <0.05. Suggestions researchers recommend is the provision of information dissemination on the importance of condom use WPS customers to suppress the incidence of STIs.

Keywords : knowledge , satisfaction , motivation , use condoms consistency
ID:4005 Injectable Cell Delivery Constructs for Myocardial Tissue Engineering
Authors: Sung Hsing-Wen , Huang Chieh-Cheng , Chen Ding-Yuan , Hsiao Chun-Wen , Lee Wen-Yu , Chang Yen
Published: September 2017

Cell transplantation via direct intramuscular injection is a promising therapy for patients with ischemic diseases. However, following injections, retention of transplanted cells in engrafted areas remains problematic, and can be deleterious to cell-transplantation therapy. In this presentation, a thermo-responsive hydrogel system composed of aqueous methylcellulose (MC) blended with phosphate-buffered saline is constructed to grow cell sheet fragments and cell bodies for the treatment of ischemic diseases. The as-prepared MC hydrogel system undergoes a sol–gel reversible transition upon heating or cooling at approximately 32 °C. Via this unique property, the grown cell sheet fragments (cell bodies) can be harvested without using proteolytic enzymes; consequently, their inherent extracellular matrices (ECMs) and integrative adhesive agents remain well preserved. In animal studies using rats and pigs with experimentally created myocardial infarction, the injected cell sheet fragments (cell bodies) become entrapped in the interstices of muscular tissues and adhere to engraftment sites, while a minimal number of cells exist in the group receiving dissociated cells. Moreover, transplantation of cell sheet fragments (cell bodies) significantly increases vascular density, thereby improving the function of an infarcted heart.

These experimental results demonstrate that cell sheet fragments (cell bodies) function as a cell-delivery construct by providing a favorable ECM environment to retain transplanted cells locally and consequently, improving the efficacy of therapeutic cell transplantation
ID:4006 Advanced Nanotechnologies for Cancer Research
Authors: Chen Peilin
Published: September 2017

In this lecture, I will discuss the recent developments in our group in the advanced optical imaging systems, nanocarriers and nanodevices for caner research. I will first discuss the development of various nanoparticles for sensing the pH value in cellular environment. By surface functionalization schemes, it is possible to control the location of nanoparticles in cells allowing us to track the local pH value around the nanoparticles inside cancer cells. As for in vivo study, we have utilized the multi-photon microscopy to investigate the disease models with the help of nanoparticles. I will discuss the enhanced permeability and retention (EPR) effect, which is a key feature of tumor blood vessels. In general, EPR-mediated passive targeting highly relies on the prolonged circulation time of nanocarriers. Particularly important two parameters, (1) nanocarrier size and (2) surface property are expected to play a key role on the pharmacokinetics and the biodistribution of the carrier material. Previously studies highlighted protein corona neutrality as an important design in the development of targeted nanomaterial delivery and demonstrated that a small difference in the surface heterogeneity could result in profoundly different interactions with cells and tissues. Therefore, the control and understanding of protein corona composition are critical for successful EPR-targeted nanomedicine. I will use mesoporous silica nanoparticles (MSN) nanoparticles as an example to illustrate the effect of size and the surface heterogeneity of MSN on their biological fate both in vitro and in vivo. I will also summarize our recent development of the circulating tumor cells (CTCs) isolation chips. CTCs are cancer cells that break away from a primary tumor or metastatic site, escape from immunosurveillance, and then circulate in the peripheral blood with the capability of forming distant metastases. The identification of CTCs in patient blood samples is technically challenging because of the extremely low concentration of CTCs among a large number of hematologic cells. To address this unmet need for rare cell isolation, we have developed substrates using the synergistic effect of nanomaterials and biological immobilization of CTC markers for enhancing CTC capture efficiency during a liquid biopsy procedure. In addition to the pursuit of high-cell-capture yield and specificity from human blood on chips, further vertical integration for the downstream characterization of CTCs is required for future CTC chips, such as liquid biopsy, for achieving a variety of clinical applications. In our approach, we have employed three-dimensional (3D) conducting polymer-based bioelectronic interfaces (BEIs) that can be integrated on electronic devices for rare circulating tumor cell (CTC) isolation, detection, and collection via an electrically triggered cell released from chips.
ID:4007 Immune-cell base for cancer therapy
Authors: Ta Van Thanh , Tran Thinh Huy , Nguyen Binh Thanh , Nguyen Linh Quy , Nguyen Hoai Quy , Tran Khanh Van
Published: September 2017

The development of immune cell-based approaches for treatment of cancer has been actively investigated for many years. One strategy that has been demonstrated as an effective method for cancer treatment is adoptive T cell therapy. The principle of this method is using Cytotoxic T lymphocytes (CTL), a crucial component of the adaptive immune system that aids in the control of intracellular pathogens. Effector CTL have the capacity to promote the apoptotic death of specifically targeted cells, using a combination of granule (perforin/granzyme)-and receptor (Fas/tumor necrosis factor)-mediated mechanisms. CTL recognize specific antigen on target cells using an unique T-cell receptor (TCR) when they are presented by class I major histocompatibility (MHC) molecules. In this study, we demonstrated that T lymphocytes were activated and dramatically expanded by stimulation with anti-CD3/CD28 antibodies and culture in the present of IL-2, IL-15 and IL-21 cytokines. These T cells exhibited a predominantly activated phenotype as manifested by an increase in the percentage of cells expressing CD8 and generation of various cytokines such as IL-2, INFγ and TNFa. These findings indicate that stimulation by anti- CD3/CD28 generated effector CTL in adoptive T-cell therapy for cancer.
ID:3001 3D bone grafting techniques in reconstruction of atrophic alveolar ridge in combination with implant placement
Authors: Vo Nhan Van , Lam Phong Dai , Truong Bao Chi , Trinh Tri Quang , Ta Quan Dong
Published: September 2017

So far, atrophic alveolar ridge defect has been remained a big challenge for implant surgeons. Correct technique indication and proper material selection plays an important role for the long term success and further complications prevention. Numerous donor site for bone reconstruction prior and during implant placement have been proposed such as autogenous, homogenous allogenous or synthetic marterial. Among them, autologous graft is considered the “gold standard” as it meet three factors for the bone augmentation’s success due to its osteogenic, osteoinductive, and osteoconductive properties that others donor site could not obtain.

 In this report, we would like to present the clinical results, the advantages and disadvantages, as well as the complications of different 3D bone grafting techniques and materials in reconstruction of severely atrophic maxilla and mandible in implant rehabilitation.
ID:3003 Increase in CD34 cells in peripheral blood following HLA randomized, ABO-matched cord blood transfusion in patients with advanced malignancy and anemia: Prognostic implications
Authors: Bhattacharya Niranjan
Published: September 2017

Cord blood is a safe alternative of adult blood for transfusion given its content of fetal and adult haemoglobin,  platelet and WBC counts, and a plasma filled with inflammatory and non-inflammatory cytokines and growth factors. It also has a hypoantigenic nature and altered metabolic profile and is rich in haematopoietic stem cells (CD34) . It has potential for treatment of anemia in diseases of different aetiologies including malignancy. 

​

STUDY DESIGN:

In the present study, 46 sex and HLA-randomized patients suffering from advanced malignancy  and anemia (Hb 8 Gm percent or less) were treated with   placental cord blood transfusion.  CD34 cells were collected from peripheral blood 72 hours after transfusion and variations were analyzed for prognostic implication. The study period was between 16 August 1999 and 16 May 2006.

​

RESULTS AND ANALYSIS:

Among the forty-six cases included in this study , Case no  2 (breast sarcoma) received the lowest amount of card blood (6 units), while Case no 46 (breast cancer) received the largest amount (32 units). The youngest patient, suffering from non-Hodgkin's lymphoma (Case 3), was a 16-year-old boy who received eight units of cord blood to combat anemia. Other patients received amounts varying from 7-15 units: Case 14 received 15 units (metachronous lymph node metastatsis), Case 19 received 14 units (breast cancer), and Case 25 received seven units (lung cancer).

​

There was no transfusion-related visible immunological or nonimmunological reaction in any patient. Studies of CD34 levels after 72 hours of transfusion showed an initial rise followed by a fall in twenty-two cases, two cases registered very little effect on the CD34 level, i.e., no change from the baseline, and the rest of the cases demonstrated a sustained rise from the baseline, i.e, 56% to 99% of peripheral mononuclear cells and a sustained high CD34 level.

​

CONCLUSION:

This preliminary study indicates that freshly collected cord blood transfusion may cause a transient transplant impact of transfused cord blood CD34 stem cells on the host without provoking clinical graft vs host disease perhaps due to a possible background structural or functional  immune suppression in advanced malignancy.

From the cord blood transfusion impact we could clinically divide the enrolled advanced cancer patients to a relatively  good (Group A)  and bad prognostic(Group B) variety of  cancer. The survival rate of patients where we detected a persistant rise of CD34 in peripheral blood after 3 units or more of transfusion of HLA randomized ABO matched and screened cord blood  was over 3 years and the cases where there are no rise of peripheral blood CD34 level in spite of repeated transfusion of 5- 32 units of cord blood died within 3 months or earlier (p value less than .01)
ID3012 Evaluation of HLA-identical sibling allogenic peripheral blood stem cell transplantation for acute myelogenous leukemia at BTH, Vietnam
Authors: Huynh Man Van
Published: September 2017

Background: the purposes of this study were to assess the efficacy of HLA-identical sibling allogenic peripheral blood stem cell transplantation in the treatment for acute myelogenous leukemia (AML) at Blood transfusion and hematology hospital at Ho Chi Minh city.

 

Methods: retrospective study, case series.

 

Results: from June 2005 to September 2016, there were 47 AML patients included 24 males and 23 females, with median age was 36 years old. Engraftment was achieved in 47 patients (100%). The median number of transfused CD34+ cells was 7.5 x 106/kg. The median time for neutrophil engraftment and platelet engraftment were 11 ± 2.8 days and 12.5 ± 10.1 days respectively. Oral mucositis occurred in 38 patients (80.9%), febrile neutropenia in 42 patients (89.4%), acute graft-versus-host disease (aGvHD) in 14 patients (29.8%), chronic graft-versus-host disease (cGvHD) in 10 patients (21.3%), and VOD in 6 patients (12.8%). Transplant-related mortality (TRM) was 4 patients (8.5%). Median follow-up time was 52.26 months. Patients remained alive were 27 (57.4%). The 5-year overall survival (OS) and disease-free survival (DFS) were 55% and 54% respectively.

 

Conclusion: HLA-identical sibling allogeneic blood stem cell transplantation can reconstruct hematopoiesis quickly and is a favorable therapeutic method for AML patients. Further development and improvement of hematopoietic stem cell transplantation techniques along with graduated and individualized therapy are expected to provide better outcomes and improved quality of life for AML patients.
ID3014 Evaluation of HER2 status in breast cancer: Current status and future directions
Authors: Salleh Mohd Nazil
Published: September 2017

Human epidermal growth factor 2, (HER2) expression is observed in approximately 20% of invasive breast cancers and is associated with poor clinical prognosis. Accurate evaluation of HER2 gene amplification status is critical for optimizing breast cancer outcomes. Evaluation of HER2 status defines the eligibility for trastuzumab therapy and significantly improves the clinical outcome in a subset of patients who test positive for HER2. Last ten years the American Society of Clinical Oncology (ASCO) and the College of American Pathologists (CAP) was developed guidelines for HER2 testing to reduce inaccuracy. However, current ASCO/CAP criteria may restrict access to HER2-target therapy for some patient groups who would derive a clear clinical benefit. In the present study, we presented preliminary data by comparing the dual-color-dual hapten-brightfield in situ hybridization (DDISH) and fluorescence in situ hybridization (FISH) from formalin-fixed paraffin embedded (FFPE) of in invasive breast cancer. A third generation, fully automated, DDISH can detect both markers on a single slide using two double stranded probes labeled with two haptens. In this report, we would like to present the clinical results, the advantages and disadvantages, as well to utilize DDISH as a tumour molecular signature application for alternative method and to improve in clinical diagnosis outcomes, recurrence prediction and individualized treatment strategies.
ID3015 Mesenchymal stem cells for treatment of chronic wounds: A Study with autologous transplantation of adipose-derived stem cells
Authors: Dinh Han Van
Published: September 2017

Objective: This study was to determine the effects of adipose-derived stem cells (ADSCs) on dermal fibroblasts responses to injury including migration and proliferation in vitro. We also evaluated the autologous transplantation of ADSCs on treatment of  human chronic wounds.

 Subjects and methods: The proliferation and migration of fibroblast was evaluated by co-culture ADSCs with allogenic dermal fibroblast and by the scratch assay. In clinical study, autologous ADSCs were transplanted on to chronic wounds of 25 patients, who were hospitalized into the Wound Healing Department of the National Institute of Burns from April, 2015 to June, 2016. The mean age was 56.88 ± 16.81, male/female ratio was 2.12. The autologous adipose-derived stem cells at passages 5 were transplanted on surface of wound every 3÷5 days. The wound biopsies for H&E staining and for Transmission Electron Microscope  were taken before transplantation and at day 7, day15 and day 20 of studied progress.

 Results: ADSCs stimulated fibroblast proliferation and migration in wound healing assay. In clinical study, before transplantation, extracellular matrix (ECM) was destroyed. After transplantation, ADSCs strongly stimulated fibroblast proliferation and fibroblasts to produce collagen. ADSCs also promoted proliferations of epithelial cells and neovascularization at the chronic wound site.

 Conclusion: Autologous ADSCs promoted the wound healing process by cell proliferation and improvement of ECM in chronic wound site.
ID3021 Acquired resistance to with EGFR tyrosine kinase inhibitors by the EGFR T790M mutation in non-small cell lung cancer
Authors: Ta Van Thanh
Published: September 2017

Mutations in the tyrosine kinase (TK) domain of the epidermal growth factor receptor (EGFR) gene respond well to treatment with EGFR tyrosine kinase inhibitors (EGFR-TKIs) including gefitinib and erlotinib in non-small cell lung cancer (NSCLC). Detection of these mutations has an important role for therapeutic decision-making in NSCLC treatment. However, all patients who experienced marked improvements with these drugs eventually developed disease progression after 10-20 months of treatment due to the acquisition of drug resistance. Approximately half of the cases with acquired resistance to EGFR-TKIs can be accounted for by a second-site mutation in exon 20 of the EGFR kinase domain T790M. In this study, 40 patients with advanced NSCLC who developed acquired resistance to EGFR-TKIs were selected. The diagnosis was defined based on the Jackman criteria for acquired resistance to EGFR-TKIs in lung cancers. Re-biopsy were performed at National cancer hospital and Oncology hospitals at Ha Noi and Ho Chi Minh City. Scorpion ARMS method was used to detect EGFR mutation status. In all, 40% (16/40) of the patients carried T790M mutation after the failure of EGFR-TKIs. The study demonstrated a critical role of molecular diagnostics for TKI acquired resistance through re-biopsies at the time of disease progression.
ID2006 Crosstalk between effector cells of adaptive immunity and mesenchymal stromal cells
Authors: Gornostaeva Aleksandra
Published: September 2017

Multipotent mesenchymal stromal cells (MSCs) are a perspective tool for regenerative medicine due low immunogenicity and immunomodulation. The "feedback loop" exists in MSC/immune cells relationships, when "inflammatory" stimulation switches immunoregulaton by MSCs. Currently, the most studied effect of allogeneic MSCs on adaptive immunity cells, mainly on T lymphocytes. Studies of the interaction of MSCs and innate immunity cells are much less. "Reverse effects" (the effect of immune cells on MSCs) are virtually not investigated. Initiation of the inflammation occurs with activation of innate immunity cells, that "turns on" immunomodulatory properties. In this regard, the study of the interaction of MSCs and monocytes is particularly relevant. MSCs from human adipose tissue and CD14+monocytes (MNs) from peripheral blood of healthy volunteers were used. To stimulate monocytes conditioned medium (CM) after 72 hours of mixed lymphocyte reaction (MLR) was applied. This CM was enriched with IL-8, INF-gamma and TNF-a.  Optimization of MN activation procedure was performed prior to experiments. CD14+MNs were incubated with different concentration of MLR-CM for a different time. The activation and viability of MNs was evaluated every 24 hours. The overnight exposure of MNs to 3-day 50% CM-MLR was found to be optimum regime. We studied the of MSC/monocyte interaction paying special attention to "feedback loop".  In the presence of activated MNs, MSCs possessed unchanged viability (96%), transmembrane mitochondria potential, ROS level and twice reduced lysosome activity. The cytokine profile in coculture medium was changed significantly. IL-6 and MCP-1 were increased vs monocultures of both cell types. IL-8 was similar to MN monoculture. TNF alpha, MIG, IL-10 were detected as tracers. Elevation of IL-6 and MIG indicates on acquisition of anti-inflammatory phenotype by MSCs. After interaction with MSC, the share of CD69+ MNs (nonspecific marker of early activation) decreased, HLA-DR (MHC class II receptor) increased slightly. A threefold increase in both CD163+ MN’s share and MFI was detected, whereas CD86 antigen was not expressed. The changes in the cytokine profile and the expression of surface markers described above are characteristic of the anti-inflammatory phenotype of monocytes.  Thus, upon interaction MSC exhibited pronounced immunomodulatory properties and shifted the phenotype of monocytes towards the anti-inflammatory. These data indicate on the MSC potential to modulate early stages of inflammation, while retaining their functional state.
ID2007 In vitro selection of macrocyclic peptides against THG-1 for Esophagus Squamous Cell Carcinomas therapeutic lead compounds
Authors: Tran Dung Thi-Phuong
Published: September 2017

Ineffective current treatments for esophagus squamous cell carcinoma (ESCC) is one of the main reasons for its low 5-year survival (20%). Transforming growth factor beta-1 stimulated clone 22 - isoform 4 (Tsc22D4/THG-1) is overexpressed in 92.6% of ESCC specimens while strictly resided in mitotically active basal layer of normal squamous epithelial tissue. Knockout of THG-1 caused reduction in cancer cells growth, invasion and tumorigenesis. Experimentally, protein-protein interactions (PPIs) were identified as THG-1 mechanism to promote cancer progression. Well-known cellular regulators', Keap1, PHD2, TBLR1 and NRBP1, functional interactions are disrupted in the present of THG-1. To develop THG-1 PPIs antagonist, Random non-standard Peptide Integrated Discovery (RaPID) system was employed. In this system, D-stereochemistry, unusual side chains and N- methylation containing macrocyclic peptides was generated and screened against THG-1. High-specificity, flat-surface binding ability, rigidity make those peptides to be more advantageous for PPIs blocking compare to small molecules and antibodies. Herein, I discuss the process to identify potential lead compounds for ESCC therapeutic by blocking THG-1 PPIs.
ID2009 H. Odorata methanol extract inhibits hepatocellular carcinoma HepG2 cells line via induction of caspase-dependent apoptosis
Authors: Nguyen Sinh Truong
Published: September 2017

Background: Globally cancer is a disease which is major burden to human health nowadays. The demand for new therapies to treat and to prevent cancer disease frequently exists. Since the toxic side effect of current treatment such as chemotherapy, the research interest is paying attention toward naturally derived-compounds because of their selective toxicity to cancer cell. This study aims to test the anticancer activity of a crude extract of Hopea odorata on HepG2 cancer cell line.

 

Method: Methanol extract were prepared from the bark of H. odorata plant. In vitro cytotoxicity Hopea odorata extract on human hepatocellular carcinoma cell line HepG2, compared to normal human cell fibroblast (HF), was investigated by Alamar Blue assay. Caspase-3/7 was detected using the reagent that consists of DEVD peptide conjugated to a nucleic acid-binding dye.  The apoptosis induction of plant extract on HepG2 was recognized by Annexin V/7AAD using flow cytometry. Disintergrated nuclei of plant-treated cell was observed under fluorescent microscope using Hoechst/PI staining. With the same technique of staining, the ratio of dead/total cells was determined by distingusing Hoechst and PI fluorescent signal.

 

Results. We found that the IC50 value of Hopea odorata extract on HepG2 is at 12.67± 5µg/ml, this value is at 44.2± 3µg/ml on HF. Vice versa, the IC50 value of Doxo on HepG2 153.3±15ng/ml while that is 6.3 ±0.6083ng/ml on HF. The selectivity index of H. Odorata extract (SI) toward HepG2 cells is approximately 3.48, while the SI of Doxorubicin toward HepG2 cells is approximately 0.04. The ratio of dead/total cells increased in dose-dependent manner when observed under fluorescent microscope, while the ratio of dead/total cells barely changed on HF cells. The plant extract inhibited HepG2 through the activation of caspase-3/7. At the concentration 250µg/ml of plant extract, 35% HepG2 cells was induced into apoptosis, and HepG2 cells appeared with disintegrated nuclei under fluorescent microscope. 

Conclusion. These finding showed methanolic extract of Hopea odorata plant induced apoptosis and selectively cytotoxic toward HepG2 rather than human fibroblast cells. Purification of compounds from Hopea odorata extract need to be performed for further research the anticancer properties of Hopea odorata.
ID2012 Helicobacter pylori infection in relation to pre-cancerous lesions in gastritis Vietnamese
Authors: Bui Truong Xuan
Published: September 2017

Gastric cancer is one of the leading cancer lesions in Vietnam. Up to now, Helicobacter pylori (H. pylori) infection is still remaining a major pathogenic factor in patients with peptic disorders in Vietnam. Aims: The aim of the study was evaluated the correlation between H. pylori infection with atrophic gastritis (AG), intestinal metaplasia (IM) and dysplasia (DP) in gastritis Vietnamese. Patients and Methods: A total of 161 gastritis patients including 105 males and 56 females with mean of age of 49.81 ± 11.32 years (21 - 79 years) were enrolled in the study. Upper GI endoscopy was evaluated in all patients and afterward gastric biopsy specimens were taken according to the recommendation of update Sydney system and modified Baylor. The gastric biopsy specimens were analyzed with skilled pathologist who did not know about clinico-endoscopic status. The confirmation of H. pylori infection was evaluated with urease test (clo-test) and Giemsa staining. Results: Of the 161 patients, 96 (59.6%) patients were infected with H. pylori, and about 72.05% (116/161) of patients was suffered from atrophic gastritis. The prevalence of atrophic gastritis in H. pylori infected patients (83/96, 86.45%) was significantly higher than that in non-infected patients (33/65, 50.76%), p = 0.041. In the study, the prevalence of intestinal metaplasia and dysplasia was 84/161 (52.17%) and 17/161 (10.55%), respectively. The prevalence of intestinal metaplasia in H. pylori infected patients was observed significantly higher than that in non-infected patients (61/96, 63.54% vs. 23/65, 35.38%, p = 0.044); and the prevalence of dysplasia in H. pylori infected patients was also higher than that in non-infected patients (14/96, 14.58% vs. 3/65, 4.61%, p = 0.073). Conclusion: In gastritis Vietnamese, H. pylori was related to atrophic gastritis, intestinal metaplasia and dysplasia, so gastritis Vietnamese infected with H. pylori could be categorized into high risk group for screening gastric cancer.
ID2020 Rs3803662 significant associated with breast cancer risk in Vietnamese population
Authors: Nguyen Thanh Thi-Ngoc
Published: September 2017

Breast cancer is one of the most common malignancies among women worldwide. GWASs have found several potential breast cancer associated SNPs in some populations. Among them, rs13281615, rs889312, rs10941679 and rs3803662 have been found to have strong relationship with breast cancer risk in different populations. Rs3803662 were statisti¬cally significantly associated with breast cancer risk in Asian population, while three others SNPs were showed to have stronger association with breast cancer in American, European and Australian than Asian. In this study, focusing on Vietnamese people only, four these SNPs were investigated to understand more about the association between these variations with breast cancer disease. An optimized genotyping method was used to genotype 600 samples including 300 controls and 300 cases. The result showed that rs13281615, rs889312, and rs10941679 were not associated with Vietnamese breast cancer patients (P>0.05). However, rs3803662 has strong association with breast cancer risk in Vietnamese population (P = 0.00122, OR = 1.454, 95%CI = 1.159-1.826). Allele A plays the role of increasing the risk of breast cancer and the genotype AA shows strongly affecting to increase breast cancer risk. Rs3803662 may involve in the list of markers using for diagnosis breast cancer in Vietnamese people in the future. A further study on larger sample size is certainly required to increase the reliability of the study. We also suggest doing more association studies for other SNPs and analyzing the haplotype to identify multiple breast cancer risk prediction markers for Vietnamese population
ID:2035 Chromosomal abnormalities and treatment response in Multiple myeloma patients at Cho Ray hospital
Authors: Suzanne Thanh Thanh , Le Oanh Hoang , Phan Xinh Thi , Tran Tung Thanh , Nguyen Cuong Kim , Phan Thang Thanh , Huynh Hoa Hong
Published: September 2017

Multiple myeloma (MM) is a malignant plasma cell, generating abnormal immunoglobulins in the blood and urin. From January 2012 to December 2014, we performed chromosome analysis and treatment for 44 MM patients with the regimens containing Bortezomib (VD, VMP, VD plus autologus hematopoietic stem cell transplantation) at the Hematology department, Cho Ray hospital. The patient median of age was 64 year old, with the proportion of male/female was 0.83. IgG and IgA were two most common M-protein with accounting for 44.7% and 22.7%, respectively. Patients were usually admitted to the hospital late in phase II and III of disease. 43 of 44 MM patient haboring chromosomal abnormalities. Karyotyping analysis results showed that 3.7% cases with hypodiploidy and 14.8% cases hyperdiploidy. Translocation t(4;14), t(6;14), t(11;14) and t(14;16) were found in 6.8%, 4.5%, 11.3% and 4.5% of cases, respectively. Del(13q) and del(17p) accounted for 31.8% and 29.5% of cases. High risk group was found in 40.9% of cases. Patients carrying the translocation t(4;14) had the worse prognosis than other abnormalities with low response rate and overall survival, whereas higher rate of mortality. Results of our study showed that chromosomal abnormalities in MM are diversity and high percentage that affect the prognosis. Therefore, we need to indentify chromosomal abnormalities before treatment to select appropriate therapy regiments.
ID:2038 THE ROLE OF THE CASPASE FAMILY in PF4-INDUCED APOPTOSIS IN BREAST CANCER IN VIVO STUDY
Authors: Din Tengku Ahmad Damitri Al Astani Tengku
Published: September 2017

Background: Apoptosis is critical to the development and homeostasis of metazoans. Apoptotic dysregulation leads to various human pathologies including cancer, autoimmune and neurodegenerative disorders. Caspases, sub-members of cysteine proteases, are the central components of apoptotic response. The ELR-negative chemokine platelet factor 4 (PF4) was initially identified as an antiangiogenic agent. It inhibits endothelial cell proliferation, migration and angiogenesis in in vitro and in vivo and was further proving in the in vivo models of human colon carcinoma and murine melanoma xenograft mouse. However, there are no studies (either in-vivo or ex-vivo) which have conclusively demonstrated and characterized the PF4 effects on inducing apoptosis in breast cancer setting and therefore, this becomes the study’s central objective.

 

Aim: This study aimed to investigate PF4's effects on the apoptosis regulation mediated by caspase -3, -6, -7, -8 and -9 in 1-methyl-1-nitrosourea-induced (MNU) invasive murine breast carcinoma model.

Methods: Breast carcinoma were induced in 60, 21-day-old female Sprague Dawley Rat (SDR) using 1-methyl-1-nitrosourea until its size reached 14.5±0.5 mm. The tumors were divided into two groups: Group 1 (control pre-treated, n=20 and post treated, n=20), and Group 2 (PF4, n=20). PF4 were administered through focal intralesional injections at 20 µg/lesion dosage. After 5 days of treatment, the SDRs were sacrificed. Subsequently the tumor specimens were prepared for haematoxylin and eosin staining. The expression of caspases family members (caspase-3,-6,-7,-8 and-9) were detected using immunohistochemistry. The specimens were morphologically examined and the cell counts with positive caspases expressions were recorded.

Results: There was a significant reduction of tumor size after PF4 treatment compared to the controls. PF4 demonstrated differential effects on downstream effector caspases; both upregulated caspase-3 and -6, and downregulated caspase-7 expression was observed compared to controls. PF4 exhibited significant effects on the three caspases-3, -6 and -7 (p <0.001, p=0.004 and p=0.024, respectively). PF4 had no effect on caspase-8 (p=0.067) and caspase-9 (p=0.061) expressions.

Conclusion: This study found that PF4 significantly reduced the tumor sizes in a short time (less than 3 days) and acts differentially on the caspase-mediated apoptosis pathways and on the downstream levels of the apoptotic machinery.
ID:2049 Effect of human umbilical cord-derived MSCs on the proliferation of peripheral blood T lymphocytes with different level HLA matching
Authors: Hoang Nhung
Published: September 2017

Human umbilical cord-derived MSCs are very attractive sources of stem cells for the non-autologous cell transplantation therapy to treat many kinds of diseases based on their efficiency in treating graft versus host disease (GVHD), a condition that patient might suffer after receive cell-transplantation. However, the immune properties of MSCs are also critical for their efficiency in regenerative medicine applications. Thus, the mechanism of their action is now under intense investigation. A number of researches showed that MSCs exert strong anti-inflammatory and immunosuppressive effects on the main immune cell subsets through their production of various soluble factors. As a result, scientists all over the globe are changing their attention to the immunomodulatory property of MSCs, hence we are not an exception. Our research was carried out in order to strengthen our knowledge about this spectacular aspect of MSCs, especially the effect of cord-derived MSCs on the proliferation of peripheral blood T lymphocytes with different level HLA matching. Our results indicated that at passage 4 of culture, human umbilical cord-derived MSCs had no effect on the proliferation of T cells with all blood sample tested.
ID:2050 Impacts of polymer-based and graphene-based biomaterials on the characteristics of Wharton jelly’s-derived mesenchymal stem cells
Authors: Vun Hiew Vun
Published: September 2017

The combination of mesenchymal stem cells (MSCs) and biomaterials scaffolds hold significant promise in tissue engineering and regenerative medicine field. In recent years, expansion and differentiation of MSCs culturing with polymer-based and graphene-based biomaterials have been intensely studied. However, the underlying molecular mechanisms are still largely unknown. This study was to examine the different characteristics of Wharton’s jelly (WJ)-derived MSCs using two different types of biomaterials including polymer-based (PU157) and graphene oxide-based (GO). In term of proliferation, WJ-MSCs culturing with PU157 and GO showed no significant differences compared to the controls. PU157 and GO had no effects on morphological features of WJ-MSCs. RT-PCR results showed that all WJ-MSCs co-culturing with or without PU157 and GO expressed positive surface markers including CD29, CD44, CD73, CD90, CD105, CD106 and CD166 but the expression of negative markers (CD34, CD45 and CD133) was undetectable. The SOX2 and RUNX2 expression were both upregulated in WJ-MSCs combining with PU157 when compared to the controls at passage 6, where no obvious difference was observed in passage 3. Interestingly, COL2A expression became more noticeable in the presence of PU157 at passage 6 than passage 3. In contrast, GO did not alter the expression of stemness, osteogenic, adipogenic and chondrogenic genes. Both alizarin red and oil red staining showed calcium and lipid deposition in WJ-MSCs co-cultured with PU157 and GO respectively, suggesting the presence of osteoblast- and adipocyte-like cells. In conclusion, PU157 exerted certain effects on the expression gene profiles at later passage and able to enhance differentiation abilities in WJ-MSCs.
ID: 1006 Allogeneic adipose -derived mesenchymal stem cell transplantion enhanced the expression of angiogenic factors on mouse acute hindlimb ischemia model
Authors: Vu Ngoc Bich , Le Ha Thi-Ngan , Dao Thuy Thi-Thanh , Phi Lan Thi , Pham Phuc Van , Phan Ngoc Kim , Ta Van Thanh
Published: September 2017

The cell migration and/or molecular mechanism in healing of damaged vascular or muscle tissue are emerging field with strong research interest worldwide. This study focuses on evaluating the role of allogenic adipose-derived mesenchymal stem cells (ADMSCs) in restoring damaged tissue through the migration of cells and the expression of genes related to myocyte regeneration and angiogenesis on hindlimb ischemic mouse model. ADMSCs were labeled with GFP (ADMSC-GFP). The proximal end of the femoral blood vessel of mice (over 6 months old) was ligated at 2 positions then cut between the two ties. Hindlimb ischemic mice were randomly divided to 2 groups. Group 1 (n=36) was injected PBS (100 μl), group 2 (n=36) was transplanted ADMSC-GFP (106 cell/100 μl PBS) near the cutting position. The migration of ADMSC-GFP in hindlimb was analyzed by UVVis system. The expression of genes related to angiogenesis and muscle tissue repairing was quantified by real time RT-PCR. Muscle tissue structure was histological analyzed by H&E staining. The result showed that ADMSC existed in grafted hindlimb during 7 days. Grafted cells migrated to other damaged areas such as thighs and heels. Ischemic hindlimb were stimulated to increasing expression of several angiogeneic genes including Flt-1, Flk-1 Ang-2 in both group. Especially, the expression of Ang-2 and myogenic-related gene MyoD were significantly increased in ADMSC-treated group compared to non-treated group; and increased at 28th day compared to 3rd day. The other factors such as HGF, CD31, Myf5, TGF-β also expressed much more in ADMSC treated group than the another one. Muscle histological structure in ADMSC group were siminar the normal mouse after 28 day. Thus, grafted-ADMSC was able to migrate to other area in injured hindlimb, existed for about 7 days and have significant positive effects on stimulating the expression of myogenic and angiogenesis- related genes.