bluuebunny/crossref_metadata_2025 huggingface dataset:
RUNX2 as a novel biomarker for early identification of patients progressing to advanced-stage mycosis fungoides
Authors: Danielsen Maria , Emmanuel Thomas , Nielsen Morten Muhlig , Lindahl Lise Maria , Gluud Maria , Ødum Niels , Raaby Line , Steiniche Torben , Iversen Lars , Bech Rikke , Buus Terkild Brink , Johansen Claus
Published: October 2024

Introduction

The majority of patients with mycosis fungoides (MF) have an indolent disease course, but a substantial fraction (20-30%) of patients progress to advanced stages – usually with a grave prognosis. Early differentiation between indolent and aggressive types of MF is important for the choice of treatment regimen and monitoring of the individual patient. Good biomarkers are therefore desired.

Methods

Here, we used spatial transcriptomics on skin samples at time-of-diagnosis to enable prediction of patients who later progressed to advanced stages of MF. Formalin-fixed, paraffin-embedded skin biopsies at time of diagnosis from six patients with MF who progressed to advanced stages of disease within 4 months to 12 years after diagnosis, and nine patients who remained in early-stage disease over 9 to 27 years were analyzed using the GeoMx Digital Spatial Profiler to capture spatially resolved high-plex RNA gene expression data. Five different regions of interest (the epidermis, the basal layer of epidermis, CD4+ T-cells and neighboring cells, and Pautrier’s microabscesses) were profiled for further assessment.

Results and discussion

Interestingly, RUNX2, SHMT2, and MCM7 were upregulated in the enriched population of malignant T-cells in Pautrier’s microabscesses in patients who later developed advanced stages of disease. Expression of RUNX2, SHMT2 and MCM7 in malignant T-cells was confirmed in a subset of patients in MF skin using scRNA-seq datasets across multiple studies and correlating with stage of disease. Taken together, we provide first evidence that RUNX2 has potential as a biomarker to identify MF patients progressing to advanced stage disease. As RUNX2 has not previously been linked to MF, our data also shows the analytical strength of combining spatial transcriptomics with scRNA-seq analysis.
SGLT2i and GLP1-RA exert additive cardiorenal protection with a RAS blocker in uninephrectomized db/db mice
Authors: Martos-Guillami Nerea , Vergara Ander , Llorens-Cebrià Carmen , Motto Aku Enam , Martínez-Díaz Irene , Gonçalves Francisco , Garcias-Ramis Maria Magdalena , Allo-Urzainqui Estibaliz , Narváez Alonso , Bermejo Sheila , Muñoz Vicent , León-Román Juan , Ferrer-Costa Roser , Jacobs-Cachá Conxita , Vilardell-Vilà Jordi , Soler María José
Published: October 2024

Introduction

Diabetic Kidney Disease (DKD) is the main cause of end-stage renal disease in the developed world. The current treatment of the DKD with renin-angiotensin system (RAS) blockade does not totally halt the progression to end stage kidney disease. Currently, several drugs have shown to delay DKD progression such as sodium-glucose co-transporter-2 inhibitors (SGLT2i) and glucagon-like-1 receptor agonists (GLP-1RA). We hypothesized that by combining several drugs that prevent DKD progression on top of RAS blockade a synergistic effect would be achieved in terms of cardiorenal protection. In the present study, we analysed if the combination of a RAS blocker (ramipril) with a SGLT2i (empagliflozin) and/or GLP-1RA (semaglutide) in a type 2 diabetic mouse model could have add-on effects in kidney and heart protection.

Methods

Male and female uninephrectomized type 2 diabetic db/db mice were treated with empagliflozin and/or semaglutide on top of ramipril during 8 weeks. During the study body weight, water and food intake were weekly monitored, glycaemia biweekly and albuminuria and glomerular filtration rate (GFR) before and after the treatment. At the end of the experiment, kidney and heart were isolated for histological and gene expression studies as well as for intrarenal RAS state assessment.

Results

Semaglutide combined with ramipril and/or empagliflozin significantly decreased albuminuria but only when combined with both compounds, semaglutide further decreased blood glucose, glomerular hyperfiltration in male mice and glomerular mesangial matrix expansion. In kidney, only the triple treatment with empagliflozin, semaglutide and ramipril reduced the expression of the proinflammatory and profibrotic genes ccl2 and TGFß1. In addition, the combination of empagliflozin and semaglutide on top of RAS blockade was superior in decreasing cardiomyocyte hypertrophy and heart fibrosis in db/db mice.

Discussion

Our results suggest that the combination of SGLT2i with GLP-1RA is superior in cardiorenal protection in DKD than the drugs administered alone on top of RAS blockade.
Dimerization and lysine substitution of melittin have differing effects on bacteria
Authors: Matthyssen Tamara , Li Wenyi , Holden James A. , Lenzo Jason C. , Hadjigol Sara , O’Brien-Simpson Neil M.
Published: October 2024

Introduction

Melittin is a potent antimicrobial peptide from bee venom that is effective against both Gram-positive and Gram-negative bacteria. However, it is extremely toxic to mammalian cells and, as yet, has no clinical use. Modifications to its amino acid sequence, cyclization, truncation, and dimerization have been attempted in order to reduce its toxicity whilst maintaining its antimicrobial activity.

Methods

In this study, we targeted the three lysine residues present in melittin and substituted them with lysine homologs containing shorter side chains (ornithine, Orn, diaminobutyric acid, Dab, and diaminopropanoic acid, Dap) and made both parallel and antiparallel melittin dimers to observe how lysine substitution and dimerization affects its activity and toxicity. The antibacterial activity of melittin and its analogs was tested against S. aureus (Gram-positive bacteria) and E. coli (Gram-negative bacteria), and cytotoxicity was tested against the mammalian cell lines HEK293 and H4IIE.

Results

Overall, dimerization and lysine substitution exhibited improved antimicrobial activity toward E. coli and limited improvement toward S. aureus. However, mammalian cell toxicity was only marginally reduced compared to native melittin. Interestingly, the parallel dimer was found to be marginally more active than the antiparallel dimer, indicating orientation maybe important for activity, although both dimers were less effective than the native and Lys-analog peptides toward S. aureus. Of the Lys substitutions, Dab and Dap improved melittin’s activity toward E. coli.

Discussion

Dimerization and Lys substitution of melittin improved the antimicrobial activity toward Gram-negative bacteria but did not significantly improve its activity toward Gram-positive bacteria. Some analogs also displayed reduced toxicity toward HEK293 and H4IIE cells but overall remained toxic at bactericidal concentrations. Our data indicates that although highly antibacterial, melittin’s toxicity is the major drawback in its potential use.
Reducing red light proportion in full-spectrum LEDs enhances runner plant propagation by promoting the growth and development of mother plants in strawberry
Authors: Chen Jian , Ji Fang , Gao Rongwei , He Dongxian
Published: October 2024

Full-spectrum light-emitting diodes (LEDs) have gradually replaced narrow-spectrum LEDs and are widely used in plant factories with artificial lighting (PFALs). However, the specific effect of LED light quality on dry mass allocation in runner plant propagation remains unclear. Hence, we cultivated “Akihime” strawberries as mother plants for 115 days to conduct runner plant propagation experiment under white LEDs (W100), white and red LEDs (W84R16 and W55R45), red and blue LEDs (RB100), and red, blue and green LEDs (RB80G20) in PFALs, and determined key factors affecting dry mass accumulation and allocation among mother plants and runner plants based on growth component analysis. The results showed that the net photosynthetic rate and total leaf area in mother plants in W100 increased by 11% and 31%, respectively, compared with W55R45. In comparison to W84R16 and W55R45, W100 increased the dry mass (23%–30%) of runner plants mainly by increasing the total dry mass (TDM) (23%) of strawberry plants, without significantly affecting the fraction of dry mass partitioning to runner plants. However, the number of runners in W55R45 was 5.1 per plant, representing only 78% of that in W100. Compared with RB100, RB80G20 significantly increased the number of runner plants and runner numbers by 16% and 19% to 13.0 per plant and 5.8 per plant, respectively. The partial replacement of blue light with green light in RB80G20 induced a shade avoidance response in runner plants, resulting in a 55% increase in the total leaf area of runner plants compared with RB100. Data from growth component analysis showed that compared with red and blue LEDs, white LEDs increased the TDM of runner plants by 83% by increasing the plant TDM accumulation (44%) and the fraction of dry mass partitioning to runner plants (37%). Additionally, the dry mass (g) of runner plants per mol and per kilowatt-hour under in W100 were 0.11 and 0.75, respectively, significantly higher than other treatments. Therefore, reducing red light proportion in full-spectrum LEDs is beneficial for strawberry runner plant propagation in PFALs.
Chemical characteristics of Salix psammophila sand barriers are accelerated degradation by ultraviolet irradiation and water
Authors: Wang Ruidong , Meng Zhongju , Gao Yong
Published: October 2024

The implementation of Salix psammophila sand barriers measures constitutes a crucial element in desertification control, providing a solid theoretical foundation for the future application and pretreatment of sand barriers in production practices. To address the specific damage types predominant in desert environments, we executed simulations of ultraviolet irradiation and rainfall phenomena on mechanical sand barriers in sandy areas and also inspected the variations in chemical properties during accelerated aging processes. The findings unequivocally demonstrate that: (1) The synergistic impact of ultraviolet irradiation and water accelerated the deformation and fracturing of the S.psammophila sand barriers, thereby causing a partial degradation of its chemical properties and conspicuous lignin oxidation; (2) The fissure of the sand barrier deepened, resulting in structural alterations. The existence of water expedites the degradation process of S.psammophila sand barriers under ultraviolet irradiation. (3)With respect to the binding form of C atoms, the carbon atoms at S.psammophila sand barriers were highly oxidized after 576 hours of accelerated aging. The components of C2 (C-O) and C3 (C=O) rising to 40.16% and 12.24% respectively, while the components of C1 (C-C) declined to 47.60%. The amount of hydroxyl (O-C-O), carbonyl (C=O), and carboxyl (O-C=O) groups increases in line with the expansion of the contact area between the sand barrier structure and ultraviolet irradiation as well as water. More free radical substances are generated, thereby causing the chemical binding properties to tend to be more stable. In summary, Ultraviolet irradiation and water change are the primary factors influencing the degradation of S.psammophila sand barriers material structure and properties. In future desertification control, it is imperative to focus on enhancing the longevity of sand barriers by ensuring their waterproofing capabilities and resistance to ultraviolet irradiation.
Elevated peripheral glutamate and upregulated expression of NMDA receptor NR1 subunit in insomnia disorder
Authors: Lin Jingjing , Hou Xiaohui , Liu Yaxi , Cai Yixian , Pan Jiyang , Liao Jiwu
Published: October 2024

Background

The present study explored the serum glutamate (Glu), glutamine (Gln), glutamic acid dehydrogenase (GAD) concentrations and the mRNA expression levels of the N-methyl-D-aspartate receptor (NMDAR) NR1 subunit in the peripheral blood of patients with insomnia disorder (ID). To our knowledge, this is the first study showing an increase in the mRNA expression levels of the NMDAR NR1 subunit in patients with ID.

Methods

This study included 30 ID patients and 30 matched healthy controls. We investigated the demographic and illness information and assessed subjective sleep quality using the Pittsburgh Sleep Quality Index. The Hamilton Depression Scale-17 and Hamilton Anxiety Scale were used to evaluate the patients’ symptoms of depression and anxiety, respectively. The quantifications of Glu, Gln and GAD concentrations were performed by Enzyme-linked immunosorbent assay (ELISA). Real-time PCR was used to detect the mRNA expression levels of the NMDAR NR1 subunit in peripheral blood.

Results

Compared with the healthy control group, the serum Glu concentrations and the mRNA expression levels of the NMDAR NR1 subunit in the ID group were significantly higher. However, there was no significant difference in Gln and GAD between the two groups. The receiver operating characteristic (ROC) analysis showed that the mRNA expression levels of the NMDAR NR1 subunit could distinguish ID patients from healthy individuals (area under the curve: 0.758; sensitivity: 73.3%; specificity: 76.7%). A negative correlation was found between the mRNA expression levels of the NMDAR NR1 subunit for age, total duration of illness, and age of first onset in the ID group, whereas a positive correlation was detected for daytime dysfunction.

Conclusion

Glutamatergic neurotransmission was abnormal in ID patients. Additionally, the mRNA expression levels of the NMDAR NR1 subunit appeared to have potential as a clinical biomarker for ID. However, the sample size of our study was limited, and future studies with larger sample sizes are needed to further validate and explore the mechanisms involved and to assess the reliability of the biomarker.
Estimating the benefit of esketamine nasal spray versus real-world treatment on patient-reported functional remission: results from the ICEBERG study
Authors: Oliveira-Maia Albino J. , Rive Benoît , Godinov Yordan , Mulhern-Haughey Siobhán
Published: October 2024

Introduction

Treatment resistant depression (TRD) affects approximately 10–30% of patients with major depressive disorder, and most patients with TRD do not respond to real-world treatments (RWT). Treatment with esketamine nasal spray (NS) plus a selective serotonin or serotonin norepinephrine reuptake inhibitor (SSRI/SNRI) has significant long-term clinical benefit over RWT in patients with TRD. However, the impact on patient-reported function remains to be determined.

Methods

The ICEBERG analysis was an indirect treatment comparison performed using data from two studies of patients with TRD: SUSTAIN-2 (esketamine NS; NCT02497287) and the European Observational TRD Cohort (EOTC; RWT; NCT03373253; clinicaltrials.gov). Here, patient−reported functional remission, assessed using the Sheehan Disability Scale (SDS), was defined as SDS ≤6 at Month 6. Analyses were conducted using propensity score re−weighting and multivariable models based on 18 covariates.

Results

At Month 6, the probability of functional remission in esketamine NS−treated patients from SUSTAIN-2 (n=512) was 25.6% (95% confidence interval [CI] 21.8–29.4), while the adjusted probability for RWT patients from the EOTC (n=184) was 11.5% (95% CI 6.9–16.1; relative risk: 2.226 [95% CI 1.451–3.416]; p=0.0003). In the total combined population (N=696), patients who did not achieve clinical response or remission had a low probability of achieving functional remission (5.84% and 8.76%, respectively). However, for patients who did achieve clinical response or remission, the probability of achieving functional remission was greater (43.38% and 54.15%, respectively), although many still did not achieve this status.

Conclusions

For patients with TRD, esketamine NS had a significant functional benefit versus RWT after 6 months of treatment. Irrespective of treatment, achievement of clinical response or remission was insufficient to attain functional remission. Nevertheless, clinical remission increased the likelihood of achieving functional remission, further supporting an important role for clinical remission in for the path towards functional recovery.
Opportunities and challenges of using generative AI to personalize educational assessment
Authors: Arslan Burcu , Lehman Blair , Tenison Caitlin , Sparks Jesse R. , López Alexis A. , Gu Lin , Zapata-Rivera Diego
Published: October 2024

In line with the positive effects of personalized learning, personalized assessments are expected to maximize learner motivation and engagement, allowing learners to show what they truly know and can do. Considering the advances in Generative Artificial Intelligence (GenAI), in this perspective article, we elaborate on the opportunities of integrating GenAI into personalized educational assessments to maximize learner engagement, performance, and access. We also draw attention to the challenges of integrating GenAI into personalized educational assessments regarding its potential risks to the assessment’s core values of validity, reliability, and fairness. Finally, we discuss possible solutions and future directions.
Dissimilar Resistance Welding of NiTi Microwires for High-Performance SMA Bundle Actuators
Authors: Scholtes Dominik , Zäh Ralf-Kilian , Faupel Benedikt , Seelecke Stefan , Motzki Paul
Published: October 2024

Shape memory alloys (SMAs) are becoming a more important factor in actuation technology. Due to their unique features, they have the potential to save weight and installation space as well as reduce energy consumption. The system integration of the generally small-diameter NiTi wires is an important cornerstone for the emerging technology. Crimping, a common method for the mechanical and electrical connection of SMA wires, has several drawbacks when it comes to miniaturization and high-force outputs. For high-force applications, for example, multiple SMA wires in parallel are needed to keep actuation frequencies high while scaling up the actuation force. To meet these challenges, the proposed study deals with the development of a resistance-welding process for manufacturing NiTi wire bundles. The wires are welded to a sheet metal substrate, resulting in promising functional properties and high joint strengths. The welding process benefits from low costs, easy-to-control parameters and good automation potential. A method for evaluating the resistance-welding process parameters is presented. With these parameters in place, a manufacturing process for bundled wire actuators is discussed and implemented. The welded joints are examined by peel tests, microscopy and fatigue experiments. The performance of the manufactured bundle actuators is demonstrated by comparison to a single wire with the same accumulated cross-sectional area.
Shock Wave and Aeroelastic Coupling in Overexpanded Nozzle
Authors: Hu Haifeng , Gao Xinni , Gao Yushan , Yang Jianwen
Published: October 2024

The growing demand for increasing the engine power of a liquid rocket is driving the development of high-power De-Laval nozzles, which is primarily achieved by increasing the expansion ratio. A high-expansion-ratio for De-Laval nozzles can cause flow separation, resulting in unsteady, asymmetric forces that can limit nozzle life. To enhance nozzle performance, various separation control methods have been proposed, but no methods have been fully implemented thus far due to the uncertainties associated with simulating flow phenomena. A numerical study of a high-area-ratio rocket engine is performed to analyze the aeroelastic performance of its structure under flow separation conditions. Based on numerical methodology, the flow inside a rocket nozzle (the VOLVO S1) is analyzed, and different separation patterns are comprehensively discussed, including both free shock separation (FSS) and restricted shock separation (RSS). Since the location of the flow separation point strongly depends on the turbulence model, both the single transport equation and two-transport-equation turbulence models are simulated, and the findings are compared with the experimental results. Therefore, the Spalart–Allmaras (SA) turbulence model is the ideal choice for this rocket nozzle geometry. A wavelet is used to analyze the amplitude frequencies from 0 to 100 Hz under various pressure fluctuation conditions. Based on a clear understanding of the flow field, an aeroelastic coupling method is carried out with loosely coupled computational fluid dynamics (CFD)/computational structural dynamics (CSD). Some insights into the aeroelasticity of the nozzle under separated flow conditions are obtained. The simulation results show the significant impact of the structural response on the inherent pressure pulsation characteristics resulting from flow separation.
SwinLabNet: Jujube Orchard Drivable Area Segmentation Based on Lightweight CNN-Transformer Architecture
Authors: Liang Mingxia , Ding Longpeng , Chen Jiangchun , Xu Liming , Wang Xinjie , Li Jingbin , Yang Hongfei
Published: October 2024

Identifying drivable areas between orchard rows is crucial for intelligent agricultural equipment. However, challenges remain in this field’s accuracy, real-time performance, and generalization of deep learning models. This study proposed the SwinLabNet model in the context of jujube orchards, an innovative network model that utilized a lightweight CNN-transformer hybrid architecture. This approach optimized feature extraction and contextual information capture, effectively addressing long-range dependencies, global information acquisition, and detailed boundary processing. After training on the jujube orchard dataset, the SwinLabNet model demonstrated significant performance advantages: training accuracy reached 97.24%, the mean Intersection over Union (IoU) was 95.73%, and the recall rate was as high as 98.36%. Furthermore, the model performed exceptionally well on vegetable datasets, highlighting its generalization capability across different crop environments. This study successfully applied the SwinLabNet model in orchard environments, providing essential support for developing intelligent agricultural equipment, advancing the identification of drivable areas between rows, and laying a solid foundation for promoting and applying intelligent agrarian technologies.
Optimized Tillage Method Increased Rice Yield in Rice Ratooning System
Authors: Yang Tingyu , Zhang Haiwei , Li Fukang , Yang Ting , Shi Yuecheng , Gu Xinyi , Chen Mingshuai , Jiang Shuochen
Published: October 2024

Ratoon rice occupies an important position in rice production owing to its time-saving, labor-saving and low-pollution planting, and increased benefits. However, the impact of tillage management on the yield in rice ratooning system has not yet been reported. Thus, field experiments were carried out to investigate the impact of seven tillage methods on the yield of ratoon rice crop in Jingzhou City in 2021–2022. The managements included winter plowing + rotary 2 times (PTw + RT2) or 3 times (PTw + RT3), spring plowing + rotary 2 times (PTs + RT2) or 3 times (PTs + RT3), no plowing + rotary 2 times (P0 + RT2) or 3 times (P0 + RT3) and no tillage (NT). PTw + RT3 had the highest total rice yield. The experimental data were collected in 2021 and 2022. In terms of main season rice yield, the order of ranking was PTw > PTs ≈ NT ≈ P0, while for ratoon rice yield, the ranking was NT > PTw ≈ PTs > P0. Generally, the root function ranked as PTw > PTs > P0 > NT. The photosynthetic capacity of the main season rice always maximized in PTw, those of the ratoon rice all maximized in NT, and those of both the main season rice and ratoon rice always minimized in P0. In the three tillage modes (PTw, PTs, P0), an additional rotary tillage did not affect the growth or yield of rice. PTw + 3RT was the highest yielding tillage management, but it is still necessary to explore other PTw + 3RT methods and more economical tillage management to increase the yield of ratoon rice.
Differentiating Growth Patterns in Winter Wheat Cultivars via Unmanned Aerial Vehicle Imaging
Authors: Atanasov Asparuh I. , Stoyanov Hristo P. , Atanasov Atanas Z.
Published: October 2024

Wheat is one of the most widely grown cereal crops, serving as a key factor in sustaining the nutritional and food balance in numerous countries. The use of non-contact methods for wheat monitoring allows for the rapid diagnosis of vegetation density, crop growth, and the presence of weeds and diseases in the investigated fields. This study aims to assess the potential for differentiating growth patterns in winter wheat cultivars by examining them with two unmanned aerial vehicles (UAVs), the Mavic 2 Pro and Phantom 4 Pro, equipped with a multispectral camera from the MAPIR™ brand. Based on an experimental study conducted in the Southern Dobruja region (Bulgaria), vegetation reflectance indices, such as the Normalized-Difference Vegetation Index (NDVI), Soil-Adjusted Vegetation Index (SAVI), and Enhanced Vegetation Index 2 (EVI2), were generated, and a database was created to track their changing trends. The obtained results showed that the values of the NDVI, EVI2, and SAVI can be used to predict the productive potential of wheat, but only after accounting for the meteorological conditions of the respective growing season. The proposed methodology provides accurate results in small areas, with a resolution of 0.40 cm/pixel when flying at an altitude of 12 m and 2.3 cm/pixel when flying at an altitude of 100 m. The achieved precision in small and ultra-small agricultural areas, at a width of 1.2 m, will help wheat breeders conduct precise diagnostics of individual wheat varieties.
Deep Learning-Enabled Dynamic Model for Nutrient Status Detection of Aquaponically Grown Plants
Authors: Taha Mohamed Farag , Mao Hanping , Mousa Samar , Zhou Lei , Wang Yafei , Elmasry Gamal , Al-Rejaie Salim , Elwakeel Abdallah Elshawadfy , Wei Yazhou , Qiu Zhengjun
Published: October 2024

Developing models to assess the nutrient status of plants at various growth stages is challenging due to the dynamic nature of plant development. Hence, this study encoded spatiotemporal information of plants within a single time-series model to precisely assess the nutrient status of aquaponically cultivated lettuce. In particular, the long short-term memory (LSTM) and deep autoencoder (DAE) approaches were combined to classify aquaponically grown lettuce plants according to their nutrient status. The proposed approach was validated using extensive sequential hyperspectral reflectance measurements acquired from lettuce leaves at different growth stages across the growing season. A DAE was used to extract distinct features from each sequential spectral dataset time step. These features were used as input to an LSTM model to classify lettuce grown across a gradient of nutrient levels. The results demonstrated that the LSTM outperformed the convolutional neural network (CNN) and multi-class support vector machine (MCSVM) approaches. Also, features selected by the DAE showed better performance compared to features extracted using both genetic algorithms (GAs) and sequential forward selection (SFS). The hybridization of deep autoencoder and long short-term memory (DAE-LSTM) obtained the highest overall classification accuracy of 94%. The suggested methodology presents a pathway to automating the process of nutrient status diagnosis throughout the entire plant life cycle, with the LSTM technique poised to assume a pivotal role in forthcoming time-series analyses for precision agriculture.
Changes in Vegetation Greenness and Responses to Land Use Changes in the Yongding River Basin (in North China) from 2002 to 2022
Authors: Zhang Dongming , Yi Mingxuan , Sun Zhengguo , Wang Yajie , Sui Kelin
Published: October 2024

Vegetation is an important component of an ecosystem, fulfilling various ecological functions in areas such as soil and water conservation, climate regulation, and water source maintenance. This study focuses on the Yongding River Basin as a research area. This study used vegetation indices with long time series as a data source in combination with Landsat land use data. This study applied linear trend estimation to analyze the interannual variation trend in vegetation greenness from 2002 to 2022 in the Yongding River Basin and quantitatively analyzed the impact of land use changes on vegetation greenness. The results show that, from 2002 to 2022, the vegetation greenness in the Yongding River Basin has shown an overall increasing trend. The average growth season and the maximum annual normalized difference vegetation index (NDVI) growth rates were 0.006/10a and 0.008/10a, respectively, and the area of increased vegetation greenness accounted for 90% of the total area. During the main growth season (April to October) in the Yongding River Basin, the NDVI generally showed a spatial pattern of being higher in mountainous areas and lower in water areas, with the largest coefficient of variation in vegetation in the river water areas, and the most stable vegetation in forest land. In terms of the changes in vegetation greenness, the contribution rate of arable land was between 36.73% and 38.63%, followed by grassland and forest land, with contribution rates of 26.86% to 27.11% and 23.94% to 26.43%, respectively. The total contribution rate of water areas, construction land, and unused land was around 10.18%. This study can provide a theoretical basis for environmental protection and rational land use in the Yongding River Basin.
Dicamba: Dynamics in Straw (Maize) and Weed Control Effectiveness
Authors: Mundt Tamara Thais , Gimenes Cotrick Gomes Giovanna Larissa , Picoli Junior Gilmar José , Lopez Ovejero Ramiro Fernando , Domingues Velini Edivaldo , Carbonari Caio Antonio
Published: October 2024

Dicamba is a post-herbicide, showing some activity in soil, and its dynamics can be influenced by several factors, including the presence of straw. Brazil has more than 50% of its production area in a no-till system; thus, a good amount of the herbicide is intercepted by the straw. This study aimed to evaluate dicamba dynamics in straw and weed control efficacy when sprayed as a PRE herbicide. For this, five different studies were conducted: we utilized different straw amounts (1) and different drought periods (2) for straw sprayed with dicamba and dicamba + glyphosate to evaluate its release from straw, different straw amounts (3), different drought periods (4), and wet and dry straw (5) to evaluate pre-emergence weed control (Bidens pilosa and Ipomoea grandifolia) and dicamba availability in medium-texture soil. Around 80% of dicamba was released from the straw after 100 mm of rainfall. One day after dicamba application, 65–70% of dicamba was released from the straw with 20 mm of rainfall, while for 7 and 14 DAA, 60% was released. Dicamba was efficient in controlling the pre-emergence of both species studied, and the amount of straw did not interfere in weed control; however, dicamba was less available in the soil after rainfall when sprayed in the straw than when sprayed directly in the soil. Up to 80% of dicamba can be released from the straw after 100 mm of rainfall and weed control was efficient for the species studied; however, the carryover effect in sensitive crops might become an issue.
An Excessive K/Na Ratio in Soil Solutions Impairs the Seedling Establishment of Sunflower (Helianthus annuus L.) through Reducing the Leaf Mg Concentration and Photosynthesis
Authors: Cheng Yu , Zhang Tibin , Gao Weiqiang , Kuang Yuxin , Liang Qing , Feng Hao , Galymzhan Saparov
Published: October 2024

In saline conditions, establishing healthy seedlings is crucial for the productivity of sunflowers (Helianthus annuus L.). Excessive potassium (K+) from irrigation water or overfertilization, similar to sodium (Na+), could adversely affect sunflower growth. However, the effects of salt stress caused by varying K/Na ratios on the establishment of sunflower seedlings have not been widely studied. We conducted a pot experiment in a greenhouse, altering the K/Na ratio of a soil solution to grow sunflower seedlings. We tested three saline solutions with K/Na ratios of 0:1 (P0S1), 1:1 (P1S1), and 1:0 (P1S0) at a constant concentration of 4 dS m−1, along with a control (CK, no salt added), with five replicates. The solutions were applied to the pots via capillary rise through small holes at the bottom. The results indicate that different K/Na ratios significantly influenced ion-selective uptake and transport in crop organs. With an increasing K/Na ratio, the K+ concentration in the roots, stems, and leaves increased, while the Na+ concentration decreased in the roots and stems, with no significant differences in the leaves. Furthermore, an excessive K/Na ratio (P1S0) suppressed the absorption and transportation of Mg2+, significantly reducing the Mg2+ concentration in the stems and leaves. A lower leaf Mg2+ concentration reduced chlorophyll concentration, impairing photosynthetic performance. The lowest plant height, leaf area, dry matter, and shoot/root ratio were observed in P1S0, with reductions of 27%, 48%, 48%, and 13% compared to CK, respectively. Compared with CK, light use efficiency and CO2 use efficiency in P1S0 were significantly reduced by 13% and 10%, respectively, while water use efficiency was significantly increased by 9%. Additionally, improved crop morphological and photosynthetic performance was observed in P1S1 and P0S1 compared with P1S0. These findings underscore the critical role of optimizing ion composition in soil solutions, especially during the sensitive seedling stage, to enhance photosynthesis and ultimately to improve the plant’s establishment. We recommend that agricultural practices in saline regions incorporate tailored irrigation and fertilization strategies that prioritize optimal K/Na ratios to maximize crop performance and sustainability.
Diversity of Marek’s Disease Virus Strains in Infections in Backyard and Ornamental Birds
Authors: Chacón Ruy D. , Sánchez-Llatas Christian J. , Astolfi-Ferreira Claudete S. , Raso Tânia Freitas , Piantino Ferreira Antonio J.
Published: October 2024

Marek’s disease is caused by Mardivirus gallidalpha2, commonly known as Marek’s disease virus (MDV). This pathogen infects various bird species resulting in a range of clinical manifestations. The meq gene, which is crucial for oncogenesis, has been extensively studied, but molecular investigations of MDV in noncommercial South American birds are limited. This study detected MDV in backyard and ornamental birds from Brazil and Peru and characterized the meq gene. MDV was confirmed in all seven outbreaks examined. Three isoforms of meq (S-meq, meq, and L-meq) and two to seven proline repeat regions (PRRs) were detected among the sequenced strains. At the amino acid level, genetic profiles with low and high virulence potential were identified. Phylogenetic analysis grouped the sequences into three distinct clusters. Selection pressure analysis revealed 18 and 15 codons under positive and negative selection, respectively. The results demonstrate significant MDV diversity in the studied birds, with both high and low virulence potentials. This study highlights the importance of monitoring and characterizing circulating MDV in backyard and ornamental birds, as they can act as reservoirs for future epidemiological outbreaks.
Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and Cordyceps militaris Extract on Acute Liver Injury Induced by LPS in Piglets
Authors: Tan Ding , Li Endian , Xiong Shijie , Sun Yue , Cheng Wenbo , Su Yong , Lu Yang
Published: October 2024

Cordyceps militaris extract (CME) contains many bioactive compounds, mainly cordycepin (CPN). This study aimed to investigate the possible mechanisms underlying the amelioration of LPS-induced acute liver injury in piglets by CME or CPN supplementation using multi-omics analysis. Twenty-four weaned piglets were randomly distributed into 4 groups (n = 6): the control and LPS groups were fed basal diets; the CPN + LPS (CPN-LPS) and CME + LPS (CME-LPS) groups were fed the basal diets supplemented with CME or CPN. The results showed that CPN or CME supplementation significantly decreased the C-reactive protein level (p < 0.05) and improved liver tissue pathology to prevent acute liver injury after LPS treatment. Compared with LPS, the transcriptomic analysis indicated that CPN supplementation significantly downregulated cell adhesion molecules, while CME supplementation significantly downregulated inflammatory mediator regulation of TRP channels, complement and coagulation cascades and cytokine-cytokine receptor interaction. The metabolomic results showed that CPN or CME supplementation significantly reduced disease biomarker of bicyclo-prostaglandin E2, and increased levels of deoxyinosine and 3-hydroxyanthranilic acid (p < 0.05). The combined transcriptome and metabolome helped identify two metabolites PC 34:2 and PC 36:0, which may be associated with the restoration of liver cell morphology. In conclusion, CPN and CME could attenuate LPS-induced acute liver injury by regulating immune-related genes and metabolites. This study elucidates the potential protective mechanism of CPN or CME supplementation against acute liver injury.
Genome-Wide Association Study of Reproductive Traits in Large White Pigs
Authors: Hong Yifeng , Tan Cheng , He Xiaoyan , Wu Dan , Zhang Yuxing , Song Changxu , Wu Zhenfang
Published: October 2024

(1) Background: Reproductive performance is crucial for the pork industry’s success. The Large White pig is central to this, yet the genetic factors influencing its reproductive traits are not well understood, highlighting the need for further research. (2) Methods: This study utilized Genome-Wide Association Studies to explore the genetic basis of reproductive traits in the Large White pig. We collected data from 2237 Large White sows across four breeding herds in southern China, focusing on eight reproductive traits. Statistical analyses included principal component analysis, linkage disequilibrium analysis, and univariate linear mixed models to identify significant single-nucleotide polymorphisms and candidate genes. (3) Results: Forty-five significantly related SNPs and 17 potential candidate genes associated with litter traits were identified. Individuals with the TT genotype at SNP rs341909772 showed an increase of 1.24 in the number of piglets born alive (NBA) and 1.25 in the number of healthy births (NHBs) compared with those with the CC genotype. (4) Conclusions: The SNPs and genes identified in this study offer insights into the genetics of reproductive traits in the Large White pig, potentially guiding the development of breeding strategies to improve litter size.
Occurrence of Encephalitozoon cuniculi and Encephalitozoon hellem in European Wild Rabbits (Oryctolagus cuniculus) in Southern Germany (Bavaria)
Authors: Breuninger Katharina , Rinder Monika , Korbel Rüdiger
Published: October 2024

Encephalitozoon cuniculi and Encephalitozoon hellem are fungus-related, obligate intracellular pathogens belonging to the microsporidia. Both microorganisms occur in mammals, birds and even humans, thus revealing a zoonotic potential. Knowledge of their relevance in wild rabbits is very limited so far. Thus, the aim of the present study was to investigate the occurrence of E. cuniculi and E. hellem in wild rabbit populations in southern Germany (Bavaria). Therefore, blood and organ samples (brain/kidney) of 158 wild rabbits were investigated by immunofluorescence and PCR-based assays. Antibodies to E. cuniculi were detected in 24 of the 158 (15.2%) wild rabbits, while DNA of E. cuniculi was found in 7 (4.4%) and DNA of E. hellem was found in 3 (1.9%). Sequencing identified E. cuniculi genotype 1. This study provides the first E. cuniculi genotype determination in free-living wild rabbits worldwide and the first evidence of E. hellem in rabbits worldwide. Wild rabbits should, therefore, be regarded as a reservoir for both pathogens and, on the basis of molecular evidence from kidney tissue and presumed urine excretion, also as a source of infection for E. cuniculi for animals and humans.
Frequency and Genetic Analysis of Porcine Circovirus Type 2, Which Circulated between 2014 and 2021 in Jiangsu, China
Authors: Xiao Qi , Qu Meng , Xie Jianping , Zhu Cigen , Shan Yuping , Mao Aihua , Qian Wenxian , Zhu Jiaping , Guo Jiahui , Lang Dong , Niu Jiaqiang , Wen Libin , He Kongwang
Published: October 2024

Porcine circovirus-associated diseases, caused by porcine circovirus type 2 (PCV2), are widespread and result in significant economic losses to the global swine industry. PCV2 can currently be divided into nine genotypes (PCV2a to PCV2i), with the currently dominant one being the PCV2d genotype. In this study, 2675 samples from 804 pig farms in 13 cities in Jiangsu Province, China, were collected between 2014 and 2021 and subjected to polymerase chain reaction analysis to investigate the frequency and genetic diversity of PCV2. The results showed that 41.42% (1108/2675) of samples tested positive for PCV2. The researchers further analyzed the genetic characteristics of 251 PCV2 strains and found that they belonged to the following four genotypes: PCV2a, PCV2b, PCV2d, and PCV2i. The dominant genotype was PCV2d, with a frequency of 49.80% (125/251). The detection rate of PCV2b was significantly higher than those of PCV2a and PCV2i, at 35.46% (89/251), 7.57% (19/251), and 7.17% (18/251), respectively. The percentage of different genotypes of PCV2 varied irregularly over time. We have further revealed the fingerprint of PCV2i genomic nucleotides for the first time. In conclusion, this study illustrates the high frequency and evolutionary features of PCV2 in Jiangsu Province over the past few years.
Altered Gut Microbiome Composition in Dogs with Hyperadrenocorticism: Key Bacterial Genera Analysis
Authors: Kang Hee-Jun , Kim Sang-Won , Kim Seon-Myung , La Tae-Min , Hyun Jae-Eun , Lee Sang-Won , Kim Jung-Hyun
Published: October 2024

Hyperadrenocorticism (HAC) is a common endocrine disorder in dogs, which is associated with diverse metabolic abnormalities. We hypothesized that elevated cortisol levels in dogs with HAC disrupt the gut microbiome (GM), and this disruption persists even after trilostane treatment. This study explored GM composition in dogs with HAC. We included 24 dogs, 15 with HAC and 9 healthy controls, and followed up with 5 dogs with HAC who received trilostane treatment. The GM analysis revealed significant compositional changes in dogs with HAC, including reduced microbiome diversity compared to healthy controls, particularly in rare taxa, as indicated by the Shannon index (p = 0.0148). Beta diversity analysis further showed a distinct clustering of microbiomes in dogs with HAC, separating them from healthy dogs (p < 0.003). Specifically, an overrepresentation of Proteobacteria (Pseudomonadota), Actinobacteria, Bacteroides, Enterococcus, Corynebacterium, Escherichia, and Proteus populations occurred alongside a decreased Firmicutes (Bacillota) population. Despite trilostane treatment, gut dysbiosis persisted in dogs with HAC at a median of 41 d post treatment, suggesting its potential role in ongoing metabolic issues. We identified GM dysbiosis in dogs with HAC by examining key bacterial genera, offering insights into potential interventions like probiotics or fecal microbiota transplants for better HAC management.
Metabolic Engineering of Glycofusion Bispecific Antibodies for α-Dystroglycanopathies
Authors: Zhong Xiaotian , Yan Guoying Grace , Chaturvedi Apurva , Li Xiuling , Gao Yijie , Girgenrath Mahasweta , Corcoran Chris J. , Diblasio-Smith Liz , LaVallie Edward R. , de Rham Teresse , Zhou Jing , Abel Molica , Riegel Logan , Lim Sean K.H. , Bloom Laird , Lin Laura , D’Antona Aaron M.
Published: October 2024

Background: α-dystroglycanopathies are congenital muscular dystrophies in which genetic mutations cause the decrease or absence of a unique and complex O-linked glycan called matriglycan. This hypoglycosylation of O-linked matriglycan on the α-dystroglycan (α-DG) protein subunit abolishes or reduces the protein binding to extracellular ligands such as laminins in skeletal muscles, leading to compromised survival of muscle cells after contraction. Methods: Surrogate molecular linkers reconnecting laminin-211 and the dystroglycan β-subunit through bispecific antibodies can be engineered to improve muscle function in the α-dystroglycanopathies. This study reports the metabolic engineering of a novel glycofusion bispecific (GBi) antibody that fuses the mucin-like domain of the α-DG to the light chain of an anti-β-DG subunit antibody. Results: Transient HEK production with the co-transfection of LARGE1, the glycoenzyme responsible for the matriglycan modification, produced the GBi antibody only with a light matriglycan modification and a weak laminin-211 binding activity. However, when a sugar feed mixture of uridine, galactose, and manganese ion (Mn2+) was added to the culture medium, the GBi antibody produced exhibited a dramatically enhanced matriglycan modification and a much stronger laminin-binding activity. Conclusions: Further investigation has revealed that Mn2+ in the sugar feeds played a critical role in increasing the matriglycan modification of the GBi antibody, key for the function of the resulting bispecific antibody.
Aged Microplastics and Antibiotic Resistance Genes: A Review of Aging Effects on Their Interactions
Authors: Tang Kuok Ho Daniel , Li Ronghua
Published: October 2024

Background: Microplastic aging affects the dynamics of antibiotic resistance genes (ARGs) on microplastics, yet no review presents the effects of microplastic aging on the associated ARGs. Objectives: This review, therefore, aims to discuss the effects of different types of microplastic aging, as well as the other pollutants on or around microplastics and the chemicals leached from microplastics, on the associated ARGs. Results: It highlights that microplastic photoaging generally results in higher sorption of antibiotics and ARGs due to increased microplastic surface area and functional group changes. Photoaging produces reactive oxygen species, facilitating ARG transfer by increasing bacterial cell membrane permeability. Reactive oxygen species can interact with biofilms, suggesting combined effects of microplastic aging on ARGs. The effects of mechanical aging were deduced from studies showing larger microplastics anchoring more ARGs due to rough surfaces. Smaller microplastics from aging penetrate deeper and smaller places and transport ARGs to these places. High temperatures are likely to reduce biofilm mass and ARGs, but the variation of ARGs on microplastics subjected to thermal aging remains unknown due to limited studies. Biotic aging results in biofilm formation on microplastics, and biofilms, often with unique microbial structures, invariably enrich ARGs. Higher oxidative stress promotes ARG transfer in the biofilms due to higher cell membrane permeability. Other environmental pollutants, particularly heavy metals, antibacterial, chlorination by-products, and other functional genes, could increase microplastic-associated ARGs, as do microplastic additives like phthalates and bisphenols. Conclusions: This review provides insights into the environmental fate of co-existing microplastics and ARGs under the influences of aging. Further studies could examine the effects of mechanical and thermal MP aging on their interactions with ARGs.