bluuebunny/crossref_metadata_2025 huggingface dataset:
Delivery of Orally Administered Digestible Antibodies Using Nanoparticles
Authors: Tashima Toshihiko
Published: March 2021

Oral administration of medications is highly preferred in healthcare owing to its simplicity and convenience; however, problems of drug membrane permeability can arise with any administration method in drug discovery and development. In particular, commonly used monoclonal antibody (mAb) drugs are directly injected through intravenous or subcutaneous routes across physical barriers such as the cell membrane, including the epithelium and endothelium. However, intravenous administration has disadvantages such as pain, discomfort, and stress. Oral administration is an ideal route for mAbs. Nonetheless, proteolysis and denaturation, in addition to membrane impermeability, pose serious challenges in delivering peroral mAbs to the systemic circulation, biologically, through enzymatic and acidic blocks and, physically, through the small intestinal epithelium barrier. A number of clinical trials have been performed using oral mAbs for the local treatment of gastrointestinal diseases, some of which have adopted capsules or tablets as formulations. Surprisingly, no oral mAbs have been approved clinically. An enteric nanodelivery system can protect cargos from proteolysis and denaturation. Moreover, mAb cargos released in the small intestine may be delivered to the systemic circulation across the intestinal epithelium through receptor-mediated transcytosis. Oral Abs in milk are transported by neonatal Fc receptors to the systemic circulation in neonates. Thus, well-designed approaches can establish oral mAb delivery. In this review, I will introduce the implementation and possibility of delivering orally administered mAbs with or without nanoparticles not only to the local gastrointestinal tract but also to the systemic circulation.
Risk of Liver Fibrosis According to TSH Levels in Euthyroid Subjects
Authors: Martínez-Escudé Alba , Pera Guillem , Rodríguez Lluís , Arteaga Ingrid , Expósito-Martínez Carmen , Torán-Monserrat Pere , Caballería Llorenç
Published: March 2021

Alterations in thyroid function may contribute to the development of liver fibrosis especially in subjects with non-alcoholic fatty liver disease. This study aimed to investigate the risk of liver fibrosis according to low-normal thyroid function in the general population. We performed a descriptive cross-sectional study in subjects from 18–75 years randomly selected from 16 primary health care centers from 2017–2019. Each subject underwent clinical evaluation, physical examination, blood analysis and transient hepatic elastography. Descriptive and multivariate logistic regression analyses were used to identify factors associated with fibrosis. We included 1096 subjects (60 ± 11 years; 61% women); 70% had strict-normal thyroid function and 30% had low-normal thyroid function. Low-normal thyroid function was associated with a higher liver stiffness (LS) values: 5.2 vs. 4.8 kPa (p = 0.001) and a greater prevalence of fibrosis: 6.1 vs. 3% (p = 0.016) and 4.3 vs. 2.1% (p = 0.044) for the cut-off points of ≥8.0 kPa and ≥9.2 kPa, respectively. After adjustment for potential confounding factors, the risk of fibrosis in subjects with low-normal thyroid function was OR 1.54 (p = 0.213). In conclusion, low-normal thyroid function is associated with higher LS values and a greater risk of liver fibrosis in the general population, being dependent on other metabolic factors.
Real-World Performance of a Self-Operated Home Monitoring System for Early Detection of Neovascular Age-Related Macular Degeneration
Authors: Ho Allen C. , Heier Jeffrey S. , Holekamp Nancy M. , Garfinkel Richard A. , Ladd Byron , Awh Carl C. , Singh Rishi P. , Sanborn George E. , Jacobs Jennifer H. , Elman Michael J. , Loewenstein Anat , Eichenbaum David A.
Published: March 2021

The real-world performance of a home telemonitoring strategy (ForeseeHome AMD Monitoring System®, Notal Vision, Inc.,Manassas VA, USA) was evaluated and compared to the device arm of the AREDS2-HOME study among patients with intermediate AMD (iAMD) who converted to neovascular AMD (nAMD). All patients with confirmed conversion to nAMD who used the home monitoring system from 10/2009 through 9/2018 were identified by Notal Vision Diagnostic Clinic’s medical records. Selected outcome variables were evaluated, including visual acuity (VA) at baseline and at conversion, and change in visual acuity (VA) from baseline to time of conversion. In total, 8991 patients performed 3,200,999 tests at a frequency of 5.6 ± 3.2 times/week. The 306 eyes that converted from iAMD to nAMD over the study period (a 2.7% annual rate) were included in the analyses. There was a median (interquartile range) change of −3.0 (0.0–(−10.0)) letters among converted eyes, 81% [95% confidence interval (72–88%)] maintained a VA ≥ 20/40 at the time of conversion, while 69% of the conversion detections were triggered by system alerts. The real-world performance of an at-home testing strategy was similar to that reported for the device arm of the AREDS2-HOME study. The home telemonitoring system can markedly increase early detection of conversion to nAMD.
Long-Term Comparison of Survival and Marginal Bone of Implants with and without Sinus Augmentation in Maxillary Molars within the Same Patients: A 5.8- to 22-Year Retrospective Study
Authors: Park Won-Bae , Han Ji-Young , Kang Kyung-Lhi
Published: March 2021

Maxillary sinus floor augmentation (MSFA) is widely used and considered a predictable procedure for implant placement. However, the influence of MSFA on implant survival and marginal bone loss (MBL) is still inconclusive. The purpose of this retrospective observational study is to evaluate the long-term genuine influence of MSFA on the survival and MBL of implants by comparing those with and without MSFA only in maxillary molars within the same patients. Thirty-eight patients (28 male and 10 female), with a total of 119 implants, received implants with and without MSFA, and were followed up for 5.8 to 22 years. Patient- and implant-related factors were assessed with a frailty model for implant survival and with generalized estimation equations (GEE) for MBL around the implant. No variables showed a statistical significance for implant failure in the frailty model. In GEE analysis for MBL, MSFA did not show any statistical significance. In conclusion, MSFA demonstrated no significant influence on implant failure and MBL in posterior maxilla in this study.
Time- and Resource-Efficient Time-to-Collision Forecasting for Indoor Pedestrian Obstacles Avoidance
Authors: Urban David , Caplier Alice
Published: March 2021

As difficult vision-based tasks like object detection and monocular depth estimation are making their way in real-time applications and as more light weighted solutions for autonomous vehicles navigation systems are emerging, obstacle detection and collision prediction are two very challenging tasks for small embedded devices like drones. We propose a novel light weighted and time-efficient vision-based solution to predict Time-to-Collision from a monocular video camera embedded in a smartglasses device as a module of a navigation system for visually impaired pedestrians. It consists of two modules: a static data extractor made of a convolutional neural network to predict the obstacle position and distance and a dynamic data extractor that stacks the obstacle data from multiple frames and predicts the Time-to-Collision with a simple fully connected neural network. This paper focuses on the Time-to-Collision network’s ability to adapt to new sceneries with different types of obstacles with supervised learning.
Determining Land Values from Residential Rents
Authors: Füss Roland , Koller Jan , Weigand Alois
Published: March 2021

The value of land is determined by the locations’ attractiveness and the degree of direct land use regulation. When regulations are binding, e.g., when a restriction on the maximum floor area ratio exists, the land price can be directly expressed as a function of the maximum floor area ratio and local amenities. We show theoretically and empirically how this approach can be used to determine land values from rental prices of residential structures built upon that land. From our empirical results, we derive two main sources for a monocentric structure of land prices. First, the location attractiveness of centrally located dwellings makes land prices more expensive. Second, as the maximum floor area ratio is high in central areas, the regulation works as a multiplier for land prices and inflates prices accordingly. Our model gives insights into the determinants of urban land prices and provides a useful approach for land appraisal in regions where land transactions are scarce.
Grammar Competition and Word Order in a Northern Early Middle English Text
Authors: Truswell Robert
Published: March 2021

The Edinburgh Royal College of Physicians manuscript of Cursor Mundi and the Northern Homilies, a northern Middle English text from the early 14th century, contains unprecedentedly high frequencies of matrix verb-third and embedded verb-second word orders with subject–verb inversion. I give a theoretical account of these word orders in terms of a grammar, the ‘CM grammar’, which differs minimally in its formal description from regular verb-second grammars, but captures these unusual word orders through addition of a second preverbal A′-projection. Despite its flexibility, the CM grammar did not spread through the English-speaking population. I discuss the theoretical consequences of this failure to spread for models of grammar competition where fitness is tied to parsing success, and discuss prospects for refining such models.
Fracture Analysis of High-Strength Screw for Highway Construction
Authors: Dubec Andrej , Kováčiková Petra , Krmela Jan , Krmelová Vladimíra , Artyukhov Artem
Published: March 2021

High-strength screws represent one of the main joining or fastening components which are commonly used in the process of installation of frame constructions for information boards or signposts, relating to the traffic roads. The control of the production process may not always be a sufficient method for ensuring road safety. The backward investigation and control of the screw material processing seems to be the one of the most important procedures when there is the occurrence of any failure during the operation of the screw. This paper is mainly focused on the analysis of the failure of the high-strength screw of 10.9 grade with M diameter of 27 × 3 and a shank length of 64 mm. The mentioned and investigated screw was used as a fastener in a highway frame construction. In the paper, there is mainly the analysis of the material for a broken screw in terms of the material micropurity, the material microstructure, the surface treatment as well as chemical composition. The evaluation was based on investigation by optical microscopy, scanning electron microscopy and energy dispersive spectroscopy. Important knowledge and results were also obtained due to information on micromorphology and material contrast of the fracture surface resulting from fractographic analysis, using the method of scanning electron microscopy. In the case of the production of the high-strength screws, the tempering stands for the decisive or crucial process of heat treatment because the given process can ensure a decrease in hardness, while the required ductile properties of the material are kept and this is also reflected in the increase of strength and micromorphology of the fracture surface. From the aspect of micropurity, inclusions of critical size or distribution were not identified in the material, referring to Czech standard ČSN ISO 4967 (420471). The microstructure corresponds to tempered martensite, but the fracture surface of the broken screw was based on an intercrystalline micromechanism, which is undesirable for the given type of component. Combined with the measurement of the HV1 (Vickers hardness at a load of 1 kg) from the edge to the central area of the screw, the analysis revealed the significant drawbacks in the heat treatment of the high-strength screw.
The Video Microscopy-Linked Electrochemical Cell: An Innovative Method to Improve Electrochemical Investigations of Biodegradable Metals
Authors: Zimmermann Tycho , Hort Norbert , Zhang Yuqiuhan , Müller Wolf-Dieter , Schwitalla Andreas
Published: March 2021

An innovative, miniature video-optical-electrochemical cell was developed and tested that allows for the conducting of electrochemical corrosion measurements and simultaneous microscopic observations over a small, well-defined surface area of corroding or degrading samples. The setup consisted of a miniature electrochemical cell that was clamped onto the metal sample and fixed under a video microscope before being filled with electrolyte. The miniature cell was comprised of afferent/efferent electrolyte ducts as well as a connection to the Mini Cell System (MCS) for electrochemical measurements. Consequently, all measured and induced currents and voltages referred to the same small area corroding completely within the field of view of the microscope, thus allowing for real-time observation and linking of surface phenomena such as hydrogen evolution and oxide deposition to electrochemical data. The experimental setup was tested on commercial purity (cp) and extra-high purity (XHP) magnesium (Mg) samples using open circuit potential and cyclic voltammetry methods under static and flowing conditions. The corrosion potential was shifted more anodically for cp Mg in comparison to XHP Mg under dynamic conditions. The corrosion current assessed from the cyclic voltametric curves were higher for the cp Mg in comparison to XHP Mg. However, there were no differences between static and flow conditions in the case of XHP Mg in contrast to cp Mg, where the current density was two times higher at dynamic conditions. The measurements and observations with this new method pave the way for a more detailed understanding of magnesium corrosion mechanisms, thus improving predictive power of electrochemical corrosion measurements on newly developed magnesium or other biodegradable alloys applied for medical devices. Different electrochemical tests can be run under various conditions, while being easy to set up and reproduce as well as being minimally destructive to the sample.
A Pressure Swing Approach to Selective CO2 Sequestration Using Functionalized Hypercrosslinked Polymers
Authors: James Alex M. , Reynolds Jake , Reed Daniel G. , Styring Peter , Dawson Robert
Published: March 2021

Functionalized hypercrosslinked polymers (HCPs) with surface areas between 213 and 1124 m2/g based on a range of monomers containing different chemical moieties were evaluated for CO2 capture using a pressure swing adsorption (PSA) methodology under humid conditions and elevated temperatures. The networks demonstrated rapid CO2 uptake reaching maximum uptakes in under 60 s. The most promising networks demonstrating the best selectivity and highest uptakes were applied to a pressure swing setup using simulated flue gas streams. The carbazole, triphenylmethanol and triphenylamine networks were found to be capable of converting a dilute CO2 stream (>20%) into a concentrated stream (>85%) after only two pressure swing cycles from 20 bar (adsorption) to 1 bar (desorption). This work demonstrates the ease with which readily synthesized functional porous materials can be successfully applied to a pressure swing methodology and used to separate CO2 from N2 from industrially applicable simulated gas streams under more realistic conditions.
Energy Utilization of Torrefied Residue from Wine Production
Authors: Tamelová Barbora , Malaťák Jan , Velebil Jan , Gendek Arkadiusz , Aniszewska Monika
Published: March 2021

A significant amount of waste is generated in the food industry, which is both an environmental and an economic problem. The recycling of this waste has become an important area of research. The processing of grapes produces 20–30% of the waste in the form of grape pomace and stalks. This article assesses the fuel values of these materials before and after torrefaction. The input materials were grape pomace samples from the varieties Riesling (Vitis vinifera “Welschriesling”) and Cabernet Sauvignon (Vitis vinifera “Cabernet Sauvignon”) from the South Moravia region and stalks from the variety Welschriesling. The torrefaction process was performed using a LECO TGA 701 thermogravimetric analyzer under nitrogen atmosphere at set temperatures of 225 °C, 250 °C, and 275 °C. The residence time was 30 min. Elemental analysis, calorific value, and gross calorific value were determined for all samples. The analyses show a positive effect of torrefaction on fuel properties in the samples. Between temperatures 250 °C and 275 °C, the carbon content increased by 4.29 wt.%, and the calorific value increased with the increase in temperature reaching a value of 25.84 MJ·kg−1 at a peak temperature of 275 °C in the sample grape pomace from blue grapevine.
Paleogeographic Characteristics of the Mengyejing Formation in the Simao Basin during Its Depositional Period and Its Indication of Potash Mineralization: A Case Study of MZK-3 Well
Authors: Lou Pengcheng , Miao Zhongying , Zheng Mianping , Zhang Xuefei , Ruan Zhuang , Xu Qihui
Published: March 2021

In China, pre-Quaternary solid potash deposit has only been discovered in the Simao Basin, and the Lower Cretaceous Mengyejing (MYJ) Formation (Fm.) is the productive layer of potash deposit. In this study, we investigated the clay conglomerates which are distributed in upper and lower members of the potash-bearing salt rock layer. We analyzed the relative contents of major elements (Al2O3, Fe2O3T, MgO, CaO, Na2O, K2O) and trace elements (B, Ba, Co, Cr, Cu, Ga, Mn, Ni, Rb, Sr, V, Zn, Zr) in the samples. The results show that MgO and CaO in the major elements are rich relative to Post Archean Australian Shale (PAAS), whose average enrichment factor values of the MgO (EFMgO) is 2.61 and CaO (EFCaO) is 4.57, and the others major elements are relatively minor; trace elements (B, Ga, Mn, Zr) are rich relative to PAAS, and the others trace elements are minor relative to PAAS. The study of paleogeographic conditions using various parameters shows that the paleoclimate is generally dry and hot during the period of clay conglomerate deposition, but it was warm and humid in certain periods; the main sedimentary environment is weak oxidation condition with strong oxidation conditions in individual periods; the average value of paleosalinity is ~21‰, and the highest is no more than ~92‰. The significance of the paleogeographic characteristics of MYJ Fm. to potash mineralization are as follows: (1) they indicates that the clay conglomerates of MYJ Fm. are not clastic sediments in brine formed by seawater, because the paleosalinity of clay conglomerates deposition period is obviously lower than that of seawater; (2) MYJ potassic salt ore is not formed by evaporation and concentration of seawater in clay conglomerates in the sedimentary basin, because there is no carbonate rock and sulfate rock of corresponding scale after the deposition of clay conglomerates in the basin; (3) clay conglomerates of MYJ Fm. were deposited in continental shallow water basin; (4) the matter source of potash minerals is deep marine strata; (5) in the MYJ Fm. sedimentation period, deep source salt moved to the surface under the background of extensional structure, and the subsequent sedimentary clastic rock formed a protective layer of potash-bearing rock, thus completing the “deep source and shallow mineralization” metallogenic process.
Magmatic Material in Sandstone Shows Prospects for New Diamond Deposits within the Northern East European Platform
Authors: Agasheva Elena
Published: March 2021

A detailed study of sandstones recovered from the upper part of the recently discovered KL-01 magmatic pipe in the southern part of the Arkhangelsk diamondiferous province (ADP), containing magmatic material and rare kimberlite indicator minerals, is presented in this paper. Results are compared to the composition of crater samples of the highly diamondiferous Vladimir Grib kimberlite pipe and several poorly to non-diamondiferous ADP pipes. To identify the type of magmatic material admixture, a model of binary mixing between country Vendian sandstones and typical ADP magmatic rocks based on correlations of La/Yb and Zr/Nb ratios and Ni contents is proposed. The modeling results show that the type of magmatic component in the KL-01 samples can be identified as kimberlite, with a maximum admixture of 20 vol.%. Kimberlite indicator mineral geochemistry did not exclude the interpretation that the composition, structure, thermal state and metasomatic enrichment of the lithospheric mantle sampled by the KL-01 pipe were suitable for the formation and preservation of diamonds. The lower boundary of the sampled lithospheric mantle could be in the depth range of 175–190 km, with a diamond window width of 55–70 km. Thus, the sandstones could represent the upper level of the crater of a new kimberlite pipe.
Thermodynamic Constraints on Smectite and Iron Oxide Formation at Gale Crater, Mars: Insights into Potential Free Energy from Aerobic Fe Oxidation in Lake Water–Groundwater Mixing Zone
Authors: Kikuchi Sakiko , Shibuya Takazo
Published: March 2021

The presence of saponite and iron oxides in Sheepbed mudstone of Yellowknife Bay at Gale crater on Mars is considered as evidence of a habitable fluvio-lacustrine environment for chemolithoautotrophy. However, the energetic availability for metabolic reactions is poorly constrained. Herein, we propose the possible mixing of surface water and groundwater that (i) explains the formation of magnetite and hematite detected in Sheepbed mudstone and (ii) may work as a potential habitable zone for aerobic Fe2+-oxidizing microbes. Our thermodynamic modeling of water–rock reactions revealed that the formation of abundant saponite in Sheepbed mudstone may occur under various conditions of water-to-rock mass ratios, temperatures (5–200 °C), and initial fluid compositions. In contrast, the formation of iron oxides in the mudstone can be explained only by the mixing of Fe2+-rich groundwater and more oxidized surface waters, where the Fe2+-rich groundwater can be generated by the low-temperature water–rock reactions with a CO2-bearing initial fluid. The calculated bioavailable energy of aerobic Fe2+ oxidation in the fluid-mixing zone on Mars is similar to that estimated for a fluid-mixing zone on Earth actually inhabited by aerobic Fe2+-oxidizing microbes. The findings will contribute to a better understanding of potential habitability on Mars.
In Vitro Study of the Fibrinolytic Activity via Single Chain Urokinase-Type Plasminogen Activator and Molecular Docking of FGFC1
Authors: Gao Chunli , Shen Quan , Tang Pengjie , Cao Yuling , Lin Houwen , Li Bailin , Sun Peng , Bao Bin , Wu Wenhui
Published: March 2021

Fungi fibrinolytic compound 1 (FGFC1) is a rare marine-derived compound that can enhance fibrinolysis both in vitro and in vivo. The fibrinolytic activity characterization of FGFC1 mediated by plasminogen (Glu-/Lys-) and a single-chain urokinase-type plasminogen activator (pro-uPA) was further evaluated. The binding sites and mode of binding between FGFC1 and plasminogen were investigated by means of a combination of in vitro experiments and molecular docking. A 2.2-fold enhancement of fibrinolytic activity was achieved at 0.096 mM FGFC1, whereas the inhibition of fibrinolytic activity occurred when the FGFC1 concentration was above 0.24 mM. The inhibition of fibrinolytic activity of FGFC1 by 6-aminohexanoic acid (EACA) and tranexamic acid (TXA) together with the docking results revealed that the lysine-binding sites (LBSs) play a crucial role in the process of FGFC1 binding to plasminogen. The action mechanism of FGFC1 binding to plasminogen was inferred, and FGFC1 was able to induce plasminogen to exhibit an open conformation by binding through the LBSs. The molecular docking results showed that docking of ligands (EACA, FGFC1) with receptors (KR1–KR5) mainly occurred through hydrophilic and hydrophobic interactions. In addition, the binding affinity values of EACA to KR1–KR5 were −5.2, −4.3, −3.7, −4.5, and −4.3 kcal/moL, respectively, and those of FGFC1 to KR1–KR5 were −7.4, −9.0, −6.3, −8.3, and −6.7 kcal/moL, respectively. The findings demonstrate that both EACA and FGFC1 bound to KR1–KR5 with moderately high affinity. This study could provide a theoretical basis for the clinical pharmacology of FGFC1 and establish a foundation for practical applications of FGFC1.
New Helvolic Acid Derivatives with Antibacterial Activities from Sarocladium oryzae DX-THL3, an Endophytic Fungus from Dongxiang Wild Rice (Oryza rufipogon Griff.)
Authors: Zhang Zhi-Bin , Du Si-Yao , Ji Bo , Ji Chang-Jiu , Xiao Yi-Wen , Yan Ri-Ming , Zhu Du
Published: March 2021

Three new helvolic acid derivatives (named sarocladilactone A (1), sarocladilactone B (2) and sarocladic acid A (3a)), together with five known compounds (6,16-diacetoxy-25-hy- droxy-3,7-dioxy-29-nordammara-1,17(20)-dien-21-oic acid (3b), helvolic acid (4), helvolinic acid (5), 6-desacetoxy-helvolic acid (6) and 1,2-dihydrohelvolic acid (7)), were isolated from the endophytic fungus DX-THL3, obtained from the leaf of Dongxiang wild rice (Oryza rufipogon Griff.). The structures of the new compounds were elucidated via HR-MS, extensive 1D and 2D NMR analysis and comparison with reported data. Compounds 1, 2, 4, 5, 6 and 7 exhibited potent antibacterial activities. In particular, sarocladilactone B (2), helvolinic acid (5) and 6-desacetoxy-helvolic acid (6) exhibited strongly Staphylococcus aureus inhibitory activity with minimum inhibitory concentration (MIC) values of 4, 1 and 4 μg/mL, respectively. The structure–activity relationship (SAR) of these compounds was primarily summarized.
Synthesis and Evaluation of Thymol-Based Synthetic Derivatives as Dual-Action Inhibitors against Different Strains of H. pylori and AGS Cell Line
Authors: Sisto Francesca , Carradori Simone , Guglielmi Paolo , Spano Mattia , Secci Daniela , Granese Arianna , Sobolev Anatoly P. , Grande Rossella , Campestre Cristina , Di Marcantonio Maria Carmela , Mincione Gabriella
Published: March 2021

Following a similar approach on carvacrol-based derivatives, we investigated the synthesis and the microbiological screening against eight strains of H. pylori, and the cytotoxic activity against human gastric adenocarcinoma (AGS) cells of a new series of ether compounds based on the structure of thymol. Structural analysis comprehended elemental analysis and 1H/13C/19F NMR spectra. The analysis of structure–activity relationships within this molecular library of 38 structurally-related compounds reported that some chemical modifications of the OH group of thymol led to broad-spectrum growth inhibition on all isolates. Preferred substitutions were benzyl groups compared to alkyl chains, and the specific presence of functional groups at para position of the benzyl moiety such as 4-CN and 4-Ph endowed the most anti-H. pylori activity toward all the strains with minimum inhibitory concentration (MIC) values up to 4 µg/mL. Poly-substitution on the benzyl ring was not essential. Moreover, several compounds characterized by the lowest minimum inhibitory concentration/minimum bactericidal concentration (MIC/MBC) values against H. pylori were also tested in order to verify a cytotoxic effect against AGS cells with respect to 5-fluorouracil and carvacrol. Three derivatives can be considered as new lead compounds alternative to current therapy to manage H. pylori infection, preventing the occurrence of severe gastric diseases. The present work confirms the possibility to use natural compounds as templates for the medicinal semi-synthesis.
On the Identification and Quantification of Ergothioneine and Lovastatin in Various Mushroom Species: Assets and Challenges of Different Analytical Approaches
Authors: Tsiantas Konstantinos , Tsiaka Thalia , Koutrotsios Georgios , Siapi Eleni , Zervakis Georgios I. , Kalogeropoulos Nick , Zoumpoulakis Panagiotis
Published: March 2021

In recent years, mushrooms have drawn the attention of agro-industries and food-industries as they were considered to be valuable natural sources of health promoting compounds such as β-glucans, ergothioneine, and lovastatin. The detection and quantification of such compounds by implementing reliable analytical approaches is of the utmost importance in order to adjust mushrooms’ cultivation conditions and maximize the production in different species. Toward this direction, the current study focuses on the comparison of ultraviolet–visible (UV–Vis) spectrometry and liquid chromatography–mass spectrometry (LC–MS) methods (a) by evaluating the content of ergothioneine and lovastatin in mushrooms and (b) by highlighting any possible substrate-based interferences that hinder the accurate determination of these two compounds in order to propose the technique-of-choice for a standardized bioactive compounds monitoring. For this purpose, mushrooms produced by three species (i.e., Agaricus bisporus, Pleurotus ostreatus, and P. citrinopileatus) on various cultivation substrates, namely wheat straw (WS), winery (grape marc (GM)), and olive oil (OL) by-products, were examined. Among the two applied techniques, the developed and validated LC–MS methods, exhibiting relatively short analysis time and higher resolution, emerge as the methods-of-choice for detecting ergothioneine and lovastatin in mushrooms. On the contrary, UV–Vis methods were hindered due to co-absorbance of different constituents, resulting in invalid results. Among the studied mushrooms, P. citrinopileatus contained the highest amount of ergothioneine (822.1 ± 20.6 mg kg−1 dry sample), whereas A. bisporus contained the highest amounts of lovastatin (1.39 ± 0.014 mg kg−1 dry sample). Regarding the effect of different cultivation substrates, mushrooms produced on OL and WS contained the highest amount of ergothioneine, while mushrooms deriving from GM-based substrates contained the highest amount of lovastatin.
Monitoring and Statistical Analysis of Formation of Organochlorine and Organobromine Compounds in Drinking Water of Different Water Intakes
Authors: Vozhdaeva Margarita Yu. , Kholova Alfiya R. , Melnitskiy Igor A. , Beloliptsev Ilya I. , Vozhdaeva Yulia S. , Kantor Evgeniy A. , Lebedev Albert T.
Published: March 2021

The main drawback of drinking water chlorination involves the formation of quite hazardous disinfection by-products (DBPs), represented mainly by halogenated species. Based on the authors’ monitoring data since 2002, the prevalence of chlorine over bromine in the composition of volatile DBPs was shown for the drinking water in Ufa (Russia). However, the situation was completely reversed in the case of semi-volatile DBPs. The principal goal of the present study involved rationalization of the results of the long-term monitoring. Gas chromatography–mass spectrometry (GC-MS) was used for the qualitative and quantitative analysis of volatile DBPs. Identification of semi-volatile compounds was carried out with GC-MS, while gas chromatography with an atomic emission detector (GC-AED) was used for their quantification. A significant contribution of oxygen to the composition of semi-volatile compounds proves the decisive role of the dissolved organic matter oxidative destructive processes. Statistical analysis revealed notable linear correlations for trihalomethane and haloacetic acid formation vs. chlorine dose. On the contrary, halogenated semi-volatile products do not demonstrate any correlations with the water quality parameters or chlorine dose. Principal component analysis (PCA) placed them into separate groups. The results allow for proposing that formation of the organohalogenated species involved the fast penetration of bromine into the humic matter molecules and, further, their oxidative destruction by active chlorine.
Transcript Regulation of the Recoded Archaeal α-l-Fucosidase In Vivo
Authors: De Lise Federica , Iacono Roberta , Strazzulli Andrea , Giglio Rosa , Curci Nicola , Maurelli Luisa , Avino Rosario , Carandente Antonio , Caliro Stefano , Tortora Alessandra , Lorenzini Fabio , Di Donato Paola , Moracci Marco , Cobucci-Ponzano Beatrice
Published: March 2021

Genetic decoding is flexible, due to programmed deviation of the ribosomes from standard translational rules, globally termed “recoding”. In Archaea, recoding has been unequivocally determined only for termination codon readthrough events that regulate the incorporation of the unusual amino acids selenocysteine and pyrrolysine, and for −1 programmed frameshifting that allow the expression of a fully functional α-l-fucosidase in the crenarchaeon Saccharolobus solfataricus, in which several functional interrupted genes have been identified. Increasing evidence suggests that the flexibility of the genetic code decoding could provide an evolutionary advantage in extreme conditions, therefore, the identification and study of interrupted genes in extremophilic Archaea could be important from an astrobiological point of view, providing new information on the origin and evolution of the genetic code and on the limits of life on Earth. In order to shed some light on the mechanism of programmed −1 frameshifting in Archaea, here we report, for the first time, on the analysis of the transcription of this recoded archaeal α-l-fucosidase and of its full-length mutant in different growth conditions in vivo. We found that only the wild type mRNA significantly increased in S. solfataricus after cold shock and in cells grown in minimal medium containing hydrolyzed xyloglucan as carbon source. Our results indicated that the increased level of fucA mRNA cannot be explained by transcript up-regulation alone. A different mechanism related to translation efficiency is discussed.
Layered Cuprates Containing Flat Fragments: High-Pressure Synthesis, Crystal Structures and Superconducting Properties
Authors: Kopnin Evgeny M.
Published: March 2021

High-pressure synthesis and crystal structures of the homologous series AuBa2(Ca,Ln)n−1CunO2n+3 (n = 1–4; Ln = rare-earth cations) are described. Their crystal structures and superconducting properties are compared with the corresponding members of the Hg-homologous series. Numerous cuprates containing flat structural fragments (CuO4, CO3 and BO3) synthesized mainly at high pressure are compared in terms of structural peculiarities and superconducting properties. Importance and future prospects of high-pressure application for the preparation of new superconducting oxides are discussed.
Comparison of Multiple Bioactive Constituents in the Corolla and Other Parts of Abelmoschus manihot
Authors: Yin Shengxin , Mei Yuqi , Wei Lifang , Zou Lisi , Cai Zhichen , Wu Nan , Yuan Jiahuan , Liu Xunhong , Ge Haitao , Wang Dianguang , Wang Dandan
Published: March 2021

Abelmoschus manihot (L.) Medic (AM), called Huangshukui in Chinese, is a widely used medicinal plant. Each part of AM has medicinal value, including Abelmoschi Radix (AR), Abelmoschi Herba (AH), Abelmoschi Folium (AF), Abelmoschi Corolla (AC), and Abelmoschi Semen (AS). However, only AC is documented in the Chinese Pharmacopoeia. In order to investigate whether there is any difference between AC and the other parts of AM, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for the simultaneous determination of 35 constituents in different parts of AM. Moreover, principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied to classify and evaluate the different parts of AM based on the content of the 35 constituents. The total contents of the 35 constituents in AC were significantly higher than in the other parts of AM and the results revealed significant differences between AC and the other parts of AM. Eight constituents were remarkably related to the sample classifications. This research does not just provide the basic information for revealing the distribution patterns in different parts of AM from the same origin, but also complements some of the scientific data for the comprehensive quality evaluation of AC.
Advances in Gold Nanoparticles-Based Colorimetric Aptasensors for the Detection of Antibiotics: An Overview of the Past Decade
Authors: Zahra Qurat ul Ain , Luo Zhaofeng , Ali Rizwan , Khan Muhammad Imran , Li Fenfen , Qiu Bensheng
Published: March 2021

Misuse of antibiotics has recently been considered a global issue because of its harmful effects on human health. Since conventional methods have numerous limitations, it is necessary to develop fast, simple, sensitive, and reproducible methods for the detection of antibiotics. Among numerous recently developed methods, aptasensors are fascinating because of their good specificity, sensitivity and selectivity. These kinds of biosensors combining aptamer with colorimetric applications of gold nanoparticles to recognize small molecules are becoming more popular owing to their advantageous features, for example, low cost, ease of use, on-site analysis ability using naked eye and no prerequisite for modern equipment. In this review, we have highlighted the recent advances and working principle of gold nanoparticles based colorimetric aptasensors as promising methods for antibiotics detection in different food and environmental samples (2011–2020). Furthermore, possible advantages and disadvantages have also been summarized for these methods. Finally, the recent challenges, outlook, and promising future perspectives for developing novel aptasensors are also considered.
Layer-Scale and Chip-Scale Transfer Techniques for Functional Devices and Systems: A Review
Authors: Gong Zheng
Published: March 2021

Hetero-integration of functional semiconductor layers and devices has received strong research interest from both academia and industry. While conventional techniques such as pick-and-place and wafer bonding can partially address this challenge, a variety of new layer transfer and chip-scale transfer technologies have been developed. In this review, we summarize such transfer techniques for heterogeneous integration of ultrathin semiconductor layers or chips to a receiving substrate for many applications, such as microdisplays and flexible electronics. We showed that a wide range of materials, devices, and systems with expanded functionalities and improved performance can be demonstrated by using these technologies. Finally, we give a detailed analysis of the advantages and disadvantages of these techniques, and discuss the future research directions of layer transfer and chip transfer techniques.
A Novel Cysteine Protease Inhibitor of Naegleria fowleri That Is Specifically Expressed during Encystation and at Mature Cysts
Authors: Lê Hương Giang , Ham A-Jeong , Kang Jung-Mi , Võ Tuấn Cường , Naw Haung , Sohn Hae-Jin , Shin Ho-Joon , Na Byoung-Kuk
Published: March 2021

Naegleria fowleri is a free-living amoeba that is ubiquitous in diverse natural environments. It causes a fatal brain infection in humans known as primary amoebic meningoencephalitis. Despite the medical importance of the parasitic disease, there is a great lack of knowledge about the biology and pathogenicity of N. fowleri. In this study, we identified and characterized a novel cysteine protease inhibitor of N. fowleri (NfCPI). NfCPI is a typical cysteine protease inhibitor belonging to the cystatin family with a Gln-Val-Val-Ala-Gly (QVVAG) motif, a characteristic motif conserved in the cystatin family of proteins. Bacterially expressed recombinant NfCPI has a dimeric structure and exhibits inhibitory activity against several cysteine proteases including cathespin Bs of N. fowleri at a broad range of pH values. Expression profiles of nfcpi revealed that the gene was highly expressed during encystation and cyst of the amoeba. Western blot and immunofluorescence assays also support its high level of expression in cysts. These findings collectively suggest that NfCPI may play a critical role in encystation or cyst formation of N. fowleri by regulating cysteine proteases that may mediate encystation or mature cyst formation of the amoeba. More comprehensive studies to investigate the roles of NfCPI in encystation and its target proteases are necessary to elucidate the regulatory mechanism and the biological significance of NfCPI.
Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes?
Authors: Sajnaga Ewa , Skowronek Marcin , Kalwasińska Agnieszka , Kazimierczak Waldemar , Ferenc Karolina , Lis Magdalena , Wiater Adrian
Published: March 2021

This study focused on the potential relationships between midgut microbiota of the common cockchafer Melolontha melolontha larvae and their resistance to entomopathogenic nematodes (EPN) infection. We investigated the bacterial community associated with control and unsusceptible EPN-exposed insects through nanopore sequencing of the 16S rRNA gene. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the most abundant bacterial phyla within the complex and variable midgut microbiota of the wild M. melolontha larvae. The core microbiota was found to include 82 genera, which accounted for 3.4% of the total number of identified genera. The EPN-resistant larvae differed significantly from the control ones in the abundance of many genera belonging to the Actinomycetales, Rhizobiales, and Clostridiales orders. Additionally, the analysis of the microbiome networks revealed different sets of keystone midgut bacterial genera between these two groups of insects, indicating differences in the mutual interactions between bacteria. Finally, we detected Xenorhabdus and Photorhabdus as gut residents and various bacterial species exhibiting antagonistic activity against these entomopathogens. This study paves the way to further research aimed at unravelling the role of the host gut microbiota on the output of EPN infection, which may contribute to enhancement of the efficiency of nematodes used in eco-friendly pest management.
Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing
Authors: Hussain Amjad , Ding Xiao , Alariqi Muna , Manghwar Hakim , Hui Fengjiao , Li Yapei , Cheng Junqi , Wu Chenglin , Cao Jinlin , Jin Shuangxia
Published: March 2021

Weeds have continually interrupted crop plants since their domestication, leading to a greater yield loss compared to diseases and pests that necessitated the practice of weed control measures. The control of weeds is crucial to ensuring the availability of sufficient food for a rapidly increasing human population. Chemical weed control (herbicides) along with integrated weed management (IWM) practices can be the most effective and reliable method of weed management programs. The application of herbicides for weed control practices calls for the urgency to develop herbicide-resistant (HR) crops. Recently, genome editing tools, especially CRISPR-Cas9, have brought innovation in genome editing technology that opens up new possibilities to provide sustainable farming in modern agricultural industry. To date, several non-genetically modified (GM) HR crops have been developed through genome editing that can present a leading role to combat weed problems along with increasing crop productivity to meet increasing food demand around the world. Here, we present the chemical method of weed control, approaches for herbicide resistance development, and possible advantages and limitations of genome editing in herbicide resistance. We also discuss how genome editing would be effective in combating intensive weed problems and what would be the impact of genome-edited HR crops in agriculture.
Charge Density Fluctuations on a Dielectric Surface Exposed to Plasma or UV Radiation
Authors: Zakharov Alexander V. , Rosenfeld Eugene V.
Published: March 2021

Dust particles on a nonconductive surface are known to acquire electric charge and detach from the surface under plasma conditions and/or when affected by ultraviolet radiation. Similar phenomena occur as a result of electrostatic surface cleaning (shedding) as well as in nature, e.g., when observing levitation of dust particles above the lunar surface. A detachment of dust particles from the surface should occur when the electrostatic forces of their repulsion Fc exceed the sum of the gravitation Fg forces and the adhesive van der Waals FvdW forces acting on the particle on a nonconducting surface. However, a paradoxical situation usually arises: the three primary forces of different nature Fc, Fg, and FvdW, acting on a speck of dust with a characteristic size of the order of hundreds or thousands of nanometers, are completely incomparable in magnitude, herewith Fc << Fg << FvdW. In the last decade, numerous attempts have been made to explain how a particle on a nonconducting surface can acquire a charge sufficient for the electrostatic forces that arise to approach the adhesive forces’ values. However, despite some successes, many questions remain unanswered. This article presents a brief analysis of the charge appearance process on a solitary dust speck and a speck lying on the surface. To explain the detachment of dust particles from the surface caused by electrostatic forces and the accumulation of a charge on those particles sufficient for levitation, one should take into account the charge density fluctuations on the surface.
Synthetic Dataset Generation of Driver Telematics
Authors: So Banghee , Boucher Jean-Philippe , Valdez Emiliano A.
Published: March 2021

This article describes the techniques employed in the production of a synthetic dataset of driver telematics emulated from a similar real insurance dataset. The synthetic dataset generated has 100,000 policies that included observations regarding driver’s claims experience, together with associated classical risk variables and telematics-related variables. This work is aimed to produce a resource that can be used to advance models to assess risks for usage-based insurance. It follows a three-stage process while using machine learning algorithms. In the first stage, a synthetic portfolio of the space of feature variables is generated applying an extended SMOTE algorithm. The second stage is simulating values for the number of claims as multiple binary classifications applying feedforward neural networks. The third stage is simulating values for aggregated amount of claims as regression using feedforward neural networks, with number of claims included in the set of feature variables. The resulting dataset is evaluated by comparing the synthetic and real datasets when Poisson and gamma regression models are fitted to the respective data. Other visualization and data summarization produce remarkable similar statistics between the two datasets. We hope that researchers interested in obtaining telematics datasets to calibrate models or learning algorithms will find our work ot be valuable.