bluuebunny/crossref_metadata_2025 huggingface dataset:
Locals and Migrants in the Coalmining Town of Enugu (Nigeria): Worker Protest and Urban Identity, 1915–1929
Authors: Brown Carolyn A.
Published: November 2015

This article focuses on the varied workforce in and around the Enugu Government Colliery, located in south-eastern Nigeria and owned by the British colonial state. Opened in 1915 at Udi and in 1917 at Iva Valley and Obwetti, the mines were in a region with a long history of slave raids, population shifts, colonization, and ensuing changes in local forms of political organization. The mines brought together an eclectic mixture of forced and voluntary unskilled labor, prisoners, unskilled contract workers, and voluntary clerical workers and artisans. Moreover, the men were from different ethno-linguistic groups. By taking into account this complex background, the article describes the gradual process by which this group of inexperienced coalminers used industrial-protest strategies that reflected their habituation to the colonial workplace. They organized strikes against the village men, who, as supervisors, exploited them in the coalmines. Their ability to reach beyond their “traditional” rural identities as “peasants” to attack the kinsmen who exploited them indicates the extent to which the complex urban and industrial environment challenged indigenous identities based on locality as well as rural status systems and gender ideologies. One of the major divisions to overcome was the one between supposedly backward “locals”, men who came from villages close to the mine, and more experienced “foreigners” coming from more distant areas in Nigeria: the work experience as “coalmen” led “locals” to see themselves as “modern men” too, and to position themselves in opposition to authoritarian village leaders. The article thus traces the contours of the challenges confronting a new working class as it experimented with unfamiliar forms of affiliation, trust, and association with people with whom it shared new, industrial experiences. It investigates the many ways that “local” men maneuvered against the authoritarian control of chiefs, forced labor, and workplace exploitation by “native” and expatriate staff.
Effect of high-potency cannabis on corpus callosum microstructure
Authors: Rigucci S. , Marques T. R. , Di Forti M. , Taylor H. , Dell'Acqua F. , Mondelli V. , Bonaccorso S. , Simmons A. , David A. S. , Girardi P. , Pariante C. M. , Murray R. M. , Dazzan P.
Published: November 2015

Background

The use of cannabis with higher Δ9-tetrahydrocannabinol content has been associated with greater risk, and earlier onset, of psychosis. However, the effect of cannabis potency on brain morphology has never been explored. Here, we investigated whether cannabis potency and pattern of use are associated with changes in corpus callosum (CC) microstructural organization, in patients with first-episode psychosis (FEP) and individuals without psychosis, cannabis users and non-users.

Method

The CC of 56 FEP (37 cannabis users) and 43 individuals without psychosis (22 cannabis users) was virtually dissected and segmented using diffusion tensor imaging tractography. The diffusion index of fractional anisotropy, mean diffusivity (MD), axial diffusivity (AD) and radial diffusivity was calculated for each segment.

Results

Across the whole sample, users of high-potency cannabis had higher total CC MD and higher total CC AD than both low-potency users and those who never used (p= 0.005 andp= 0.004, respectively). Daily users also had higher total CC MD and higher total CC AD than both occasional users and those who never used (p= 0.001 andp< 0.001, respectively). However, there was no effect of group (patient/individuals without psychosis) or group x potency interaction for either potency or frequency of use. The within-group analysis showed in fact that the effects of potency and frequency were similar in FEP users and in users without psychosis.

Conclusions

Frequent use of high-potency cannabis is associated with disturbed callosal microstructural organization in individuals with and without psychosis. Since high-potency preparations are now replacing traditional herbal drugs in many European countries, raising awareness about the risks of high-potency cannabis is crucial.
GNSS-based Orbital Filter for Earth Moon Transfer Orbits
Authors: Capuano Vincenzo , Basile Francesco , Botteron Cyril , Farine Pierre- André
Published: November 2015

Numerous applications, not only Earth-based, but also space-based, have strengthened the interest of the international scientific community in using Global Navigation Satellite Systems (GNSSs) as navigation systems for space missions that require good accuracy and low operating costs. Indeed, already successfully used in Low Earth Orbits (LEOs), GNSS-based navigation systems can maximise the autonomy of a spacecraft while reducing the burden and the costs of ground operations. That is why GNSS is also attractive for applications in higher Earth orbits up to the Moon, such as in Moon Transfer Orbits (MTOs). However, the higher the altitude the receiver is above the GNSS constellations, the poorer and the weaker are the relative geometry and the received signal powers, respectively, leading to a significant navigation accuracy reduction. In order to improve the achievable GNSS performance in MTOs, we consider in this paper an adaptive orbital filter that fuses the GNSS observations with an orbital forces model. Simulation results show a navigation accuracy significantly higher than that attainable individually by a standalone GNSS receiver or by means of a pure orbital propagation.
Identifying the temperamental roots of children's patterns of security in the interparental relationship
Authors: Davies Patrick T. , Hentges Rochelle F. , Sturge-Apple Melissa L.
Published: November 2015

Guided by emotional security theory, this study examined the temperamental precursors of distinctive profiles of children's responses to interparental conflict. Participants included 243 children (M = 4.6 years) and their parents across two annual measurement occasions. Temperamental constructs of frustration proneness, approach, positive affect, activity level, and effortful control were assessed through multiple methods, informants, and contexts. Behavioral observations of children's responses to interparental conflict at each wave yielded four profiles: secure (i.e., efficiently address direct threat), mobilizing (i.e., vigilance to potential threat and social opportunities), dominant (i.e., directly defeat threat), and demobilizing (i.e., reduce salience as a target of hostility). Results supported hypotheses on the distinct constellations of temperament in predicting subsequent change in the four security profiles.
THE SPIRIT OF REFORM: RELIGIOUS IDEAS AND SOCIAL CHANGE IN MODERN AMERICA
Authors: KOSEK JOSEPH KIP
Published: November 2015

Intellectual historians have long appreciated the central role of religious ideas in the movements for social transformation that shaped the early United States. The American Revolution, the nation's original radical event, has for generations sparked investigations into the relationship between evangelical theology and political consciousness. The antebellum period has probably inspired more scholarship on the social significance of religion than any other era, most notably in sensitive and detailed accounts of the antislavery movement, but also in studies of prison reform, public schools, the treatment of people with disabilities, and much else.
Using Exercise to Fight Depression in Older Adults
Authors: Heinzel Stephan , Lawrence Jimmy B. , Kallies Gunnar , Rapp Michael A. , Heissel Andreas
Published: November 2015

Abstract. Depression is the most prevalent psychiatric disorder in the general population. Despite a large demand for efficient treatment options, the majority of older depressed adults does not receive adequate treatment: Additional low-threshold treatments are needed for this age group. Over the past two decades, a growing number of randomized controlled trials (RCT) have been conducted, testing the efficacy of physical exercise in the alleviation of depression in older adults. This meta-analysis systematically reviews and evaluates these studies; some subanalyses testing specific effects of different types of exercise and settings are also performed. In order to be included, exercise programs of the RCTs had to fulfill the criteria of exercise according to the American College of Sports Medicine, including a sample mean age of 60 or above and an increased level of depressive symptoms. Eighteen trials with 1,063 participants fulfilled our inclusion criteria. A comparison of the posttreatment depression scores between the exercise and control groups revealed a moderate effect size in favor of the exercise groups (standardized mean difference (SMD) of –0.68, p < .001). The effect was comparable to the results achieved when only the eleven trials with low risk of bias were included (SMD = –0.63, p < .001). The subanalyses showed significant effects for all types of exercise and for supervised interventions. The results of this meta-analysis suggest that physical exercise may serve as a feasible, additional intervention to fight depression in older adults. However, because of small sample sizes of the majority of individual trials and high statistical heterogeneity, results must be interpreted carefully.
The ultrastructure of subgingival dental plaque, revealed by high-resolution field emission scanning electron microscopy
Authors: Holliday Richard , Preshaw Philip M , Bowen Leon , Jakubovics Nicholas S
Published: November 2015

Objectives/Aims:

To explore the ultrastructure of subgingival dental plaque using high-resolution field emission scanning electron microscopy (FE-SEM) and to investigate whether extracellular DNA (eDNA) could be visualised in ex vivo samples.

Materials and Methods:

Ten patients were recruited who fulfilled the inclusion criteria (teeth requiring extraction with radiographic horizontal bone loss of over 50% and grade II/III mobility). In total, 12 teeth were extracted using a minimally traumatic technique. Roots were sectioned using a dental air turbine handpiece, under water cooling to produce 21 samples. Standard fixation and dehydration protocols were followed. For some samples, gold-labelled anti-DNA antibodies were applied before visualising biofilms by FE-SEM.

Results:

High-resolution FE-SEMs of subgingival biofilm were obtained in 90% of the samples. The sectioning technique left dental plaque biofilms undisturbed. Copious amounts of extracellular material were observed in the plaque, which may have been eDNA as they had a similar appearance to labelled eDNA from in vitro studies. There was also evidence of membrane vesicles and open-ended tubular structures. Efforts to label eDNA with immune-gold antibodies were unsuccessful and eDNA was not clearly labelled.

Conclusions:

High-resolution FE-SEM images were obtained of undisturbed subgingival ex vivo dental plaque biofilms. Important structural features were observed including extracellular polymeric material, vesicles and unusual open tubule structures that may be remnants of lysed cells. The application of an eDNA immune-gold-labelling technique, previously used successfully in in vitro samples, did not clearly identify eDNA in ex vivo samples. Further studies are needed to characterise the molecular composition of the observed extracellular matrix material.
Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells
Authors: Oliveira L M A , Falomir-Lockhart L J , Botelho M G , Lin K-H , Wales P , Koch J C , Gerhardt E , Taschenberger H , Outeiro T F , Lingor P , Schüle B , Arndt-Jovin D J , Jovin T M
Published: November 2015

We have assessed the impact of α-synuclein overexpression on the differentiation potential and phenotypic signatures of two neural-committed induced pluripotent stem cell lines derived from a Parkinson's disease patient with a triplication of the human SNCA genomic locus. In parallel, comparative studies were performed on two control lines derived from healthy individuals and lines generated from the patient iPS-derived neuroprogenitor lines infected with a lentivirus incorporating a small hairpin RNA to knock down the SNCA mRNA. The SNCA triplication lines exhibited a reduced capacity to differentiate into dopaminergic or GABAergic neurons and decreased neurite outgrowth and lower neuronal activity compared with control cultures. This delayed maturation phenotype was confirmed by gene expression profiling, which revealed a significant reduction in mRNA for genes implicated in neuronal differentiation such as delta-like homolog 1 (DLK1), gamma-aminobutyric acid type B receptor subunit 2 (GABABR2), nuclear receptor related 1 protein (NURR1), G-protein-regulated inward-rectifier potassium channel 2 (GIRK-2) and tyrosine hydroxylase (TH). The differentiated patient cells also demonstrated increased autophagic flux when stressed with chloroquine. We conclude that a two-fold overexpression of α-synuclein caused by a triplication of the SNCA gene is sufficient to impair the differentiation of neuronal progenitor cells, a finding with implications for adult neurogenesis and Parkinson’s disease progression, particularly in the context of bioenergetic dysfunction.
A novel cyclic helix B peptide inhibits dendritic cell maturation during amelioration of acute kidney graft rejection through Jak-2/STAT3/SOCS1
Authors: Yang C , Zhang Y , Wang J , Li L , Wang L , Hu M , Xu M , Long Y , Rong R , Zhu T
Published: November 2015

We recently synthesized a novel proteolysis-resistant cyclic helix B peptide (CHBP) that exhibits promising renoprotective effects. Dendritic cells (DCs) play an activation role in acute rejection (AR). Thus, the present study was designed to investigate the effects of CHBP on DCs in a rat renal transplantation model. The left kidney was harvested from male Lewis rats and then transplanted into male Wistar rats with or without CHBP treatment. Five successive treatment doses of CHBP after transplantation significantly ameliorated AR with lower histological injury, apoptosis and CD4+ and CD8+ T-cell infiltration in renal allografts. CHBP reduced IFN-γ and IL-1β levels but increased IL-4 and IL-10 levels in the serum. The number of mature DCs was significantly decreased in renal allografts treated with CHBP. In addition, incubating DCs with CHBP in vitro led to reduction in TNF-α, IFN-γ, IL-1β and IL-12 levels and increase of IL-10 expression at the protein level in the supernatant. Mechanistically, CHBP inhibited TLR activation-induced DC maturation by increasing SOCS1 expression through Jak-2/STAT3 signaling. In conclusion, CHBP suppresses renal allograft AR by inhibiting the maturation of DCs via Jak-2/STAT3/SOCS1 signaling, suggesting that CHBP may be an potential therapeutic drug for treating renal AR.
High levels of heterogeneity in diazotroph diversity and activity within a putative hotspot for marine nitrogen fixation
Authors: Messer Lauren F , Mahaffey Claire , M Robinson Charlotte , Jeffries Thomas C , Baker Kirralee G , Bibiloni Isaksson Jaime , Ostrowski Martin , Doblin Martina A , Brown Mark V , Seymour Justin R
Published: November 2015

Australia’s tropical waters represent predicted ‘hotspots’ for nitrogen (N2) fixation based on empirical and modelled data. However, the identity, activity and ecology of diazotrophs within this region are virtually unknown. By coupling DNA and cDNA sequencing of nitrogenase genes (nifH) with size-fractionated N2 fixation rate measurements, we elucidated diazotroph dynamics across the shelf region of the Arafura and Timor Seas (ATS) and oceanic Coral Sea during Austral spring and winter. During spring, Trichodesmium dominated ATS assemblages, comprising 60% of nifH DNA sequences, while Candidatus Atelocyanobacterium thalassa (UCYN-A) comprised 42% in the Coral Sea. In contrast, during winter the relative abundance of heterotrophic unicellular diazotrophs (δ-proteobacteria and γ-24774A11) increased in both regions, concomitant with a marked decline in UCYN-A sequences, whereby this clade effectively disappeared in the Coral Sea. Conservative estimates of N2 fixation rates ranged from <1 to 91 nmol l−1 day−1, and size fractionation indicated that unicellular organisms dominated N2 fixation during both spring and winter, but average unicellular rates were up to 10-fold higher in winter than in spring. Relative abundances of UCYN-A1 and γ-24774A11 nifH transcripts negatively correlated to silicate and phosphate, suggesting an affinity for oligotrophy. Our results indicate that Australia’s tropical waters are indeed hotspots for N2 fixation and that regional physicochemical characteristics drive differential contributions of cyanobacterial and heterotrophic phylotypes to N2 fixation.
Large increases in carbon burial in northern lakes during the Anthropocene
Authors: Heathcote Adam J. , Anderson N. John , Prairie Yves T. , Engstrom Daniel R. , del Giorgio Paul A.
Published: November 2015

Northern forests are important ecosystems for carbon (C) cycling and lakes within them process and bury large amounts of organic-C. Current burial estimates are poorly constrained and may discount other shifts in organic-C burial driven by global change. Here we analyse a suite of northern lakes to determine trends in organic-C burial throughout the Anthropocene. We found burial rates increased significantly over the last century and are up to five times greater than previous estimates. Despite a correlation with temperature, warming alone did not explain the increase in burial, suggesting the importance of other drivers including atmospherically deposited reactive nitrogen. Upscaling mean lake burial rates for each time period to global northern forests yields up to 4.5 Pg C accumulated in the last 100 years—20% of the total burial over the Holocene. Our results indicate that lakes will become increasingly important for C burial under future global change scenarios.
An in cellulo-derived structure of PAK4 in complex with its inhibitor Inka1
Authors: Baskaran Yohendran , Ang Khay C. , Anekal Praju V. , Chan Wee L. , Grimes Jonathan M. , Manser Ed , Robinson Robert C.
Published: November 2015

PAK4 is a metazoan-specific kinase acting downstream of Cdc42. Here we describe the structure of human PAK4 in complex with Inka1, a potent endogenous kinase inhibitor. Using single mammalian cells containing crystals 50 μm in length, we have determined the in cellulo crystal structure at 2.95 Å resolution, which reveals the details of how the PAK4 catalytic domain binds cellular ATP and the Inka1 inhibitor. The crystal lattice consists only of PAK4–PAK4 contacts, which form a hexagonal array with channels of 80 Å in diameter that run the length of the crystal. The crystal accommodates a variety of other proteins when fused to the kinase inhibitor. Inka1–GFP was used to monitor the process crystal formation in living cells. Similar derivatives of Inka1 will allow us to study the effects of PAK4 inhibition in cells and model organisms, to allow better validation of therapeutic agents targeting PAK4.
Pie-like electrode design for high-energy density lithium–sulfur batteries
Authors: Li Zhen , Zhang Jin Tao , Chen Yu Ming , Li Ju , Lou Xiong Wen
Published: November 2015

Owing to the overwhelming advantage in energy density, lithium–sulfur (Li–S) battery is a promising next-generation electrochemical energy storage system. Despite many efforts in pursuing long cycle life, relatively little emphasis has been placed on increasing the areal energy density. Herein, we have designed and developed a ‘pie’ structured electrode, which provides an excellent balance between gravimetric and areal energy densities. Combining lotus root-like multichannel carbon nanofibers ‘filling’ and amino-functionalized graphene ‘crust’, the free-standing paper electrode (S mass loading: 3.6 mg cm−2) delivers high specific capacity of 1,314 mAh g−1(4.7 mAh cm−2) at 0.1 C (0.6 mA cm−2) accompanied with good cycling stability. Moreover, the areal capacity can be further boosted to more than 8 mAh cm−2by stacking three layers of paper electrodes with S mass loading of 10.8 mg cm−2.
Enhanced spin-phonon-electronic coupling in a 5d oxide
Authors: Calder S. , Lee J. H. , Stone M. B. , Lumsden M. D. , Lang J. C. , Feygenson M. , Zhao Z. , Yan J.-Q. , Shi Y. G. , Sun Y. S. , Tsujimoto Y. , Yamaura K. , Christianson A. D.
Published: November 2015

Enhanced coupling of material properties offers new fundamental insights and routes to multifunctional devices. In this context 5d oxides provide new paradigms of cooperative interactions that drive novel emergent behaviour. This is exemplified in osmates that host metal–insulator transitions where magnetic order appears intimately entwined. Here we consider such a material, the 5d perovskite NaOsO3, and observe a coupling between spin and phonon manifested in a frequency shift of 40 cm−1, the largest measured in any material. The anomalous modes are shown to involve solely Os–O interactions and magnetism is revealed as the driving microscopic mechanism for the phonon renormalization. The magnitude of the coupling in NaOsO3 is primarily due to a property common to all 5d materials: the large spatial extent of the ion. This allows magnetism to couple to phonons on an unprecedented scale and in general offers multiple new routes to enhanced coupled phenomena in 5d materials.
Nanoscale measurements of unoccupied band dispersion in few-layer graphene
Authors: Jobst Johannes , Kautz Jaap , Geelen Daniël , Tromp Rudolf M. , van der Molen Sense Jan
Published: November 2015

The properties of any material are fundamentally determined by its electronic band structure. Each band represents a series of allowed states inside a material, relating electron energy and momentum. The occupied bands, that is, the filled electron states below the Fermi level, can be routinely measured. However, it is remarkably difficult to characterize the empty part of the band structure experimentally. Here, we present direct measurements of unoccupied bands of monolayer, bilayer and trilayer graphene. To obtain these, we introduce a technique based on low-energy electron microscopy. It relies on the dependence of the electron reflectivity on incidence angle and energy and has a spatial resolution ∼10 nm. The method can be easily applied to other nanomaterials such as van der Waals structures that are available in small crystals only.
Universal mechanisms of sound production and control in birds and mammals
Authors: Elemans C.P.H , Rasmussen J.H. , Herbst C.T. , Düring D.N. , Zollinger S.A. , Brumm H. , Srivastava K. , Svane N. , Ding M. , Larsen O.N. , Sober S.J. , Švec J.G.
Published: November 2015

As animals vocalize, their vocal organ transforms motor commands into vocalizations for social communication. In birds, the physical mechanisms by which vocalizations are produced and controlled remain unresolved because of the extreme difficulty in obtaining in vivo measurements. Here, we introduce an ex vivo preparation of the avian vocal organ that allows simultaneous high-speed imaging, muscle stimulation and kinematic and acoustic analyses to reveal the mechanisms of vocal production in birds across a wide range of taxa. Remarkably, we show that all species tested employ the myoelastic-aerodynamic (MEAD) mechanism, the same mechanism used to produce human speech. Furthermore, we show substantial redundancy in the control of key vocal parameters ex vivo, suggesting that in vivo vocalizations may also not be specified by unique motor commands. We propose that such motor redundancy can aid vocal learning and is common to MEAD sound production across birds and mammals, including humans.
Clinicopathologic significance of legumain overexpression in cancer: a systematic review and meta-analysis
Authors: Zhen Ye , Chunlei Guo , Wenzhi Shen , Shuangtao Zhao , Na Luo , Rongrong Wang , Xiaohe Luo , Haiying Niu , Dehong Luo , Shan Jiang , Xiaoyue Tan , Rong Xiang
Published: November 2015

Since reports on the clinical significance of legumain in cancer have shown inconsistent results, we systematically evaluated clinical indicators of legumain in cancer. We searched the Cochrane Library, PubMed, Embase and EBSCO databases and the Wangfang and CNKI databases in China by using “legumain” and (“neoplasms” OR “cancer”) as search terms. We included case-controlled studies of legumain and cancer. The quality of the studies was evaluated by using Lichtenstein’s guidelines and valid data was extracted for analysis. In total, 10 articles were included in this study. Meta-analysis showed that legumain was overexpressed in cancer compared with in normal tissue and was higher in stage III–IV disease than in I–II disease. Moreover, legumain overexpression was correlated with poor prognosis and clinical stage. Furthermore, Cancer Genome Atlas data showed that among patients with rectal cancer, those with tumors overexpressing legumain had shorter overall survival than those in the low expression group (P < 0.05). Legumain appears to be involved in tumor development and deterioration; thus, it can potentially be developed into both a marker for monitoring and diagnosing tumors and a therapeutic target.
Enhanced or Weakened Western North Pacific Subtropical High under Global Warming?
Authors: He Chao , Zhou Tianjun , Lin Ailan , Wu Bo , Gu Dejun , Li Chunhui , Zheng Bin
Published: November 2015

The Western North Pacific Subtropical High (WNPSH) regulates East Asian climate in summer. Anomalous WNPSH causes floods, droughts and heat waves in China, Japan and Korea. The potential change of the WNPSH under global warming is concerned by Asian people, but whether the WNPSH would be enhanced or weakened remains inconclusive. Based on the multi-model climate change projection from the 5th phase of Coupled Model Intercomparison Project (CMIP5), we show evidences that the WNPSH tends to weaken and retreat eastward in the mid-troposphere in response to global warming, accompanied by an eastward expansion of East Asian rain belt along the northwestern flank of WNPSH. Weakened meridional temperature gradient on the northern flank of WNPSH and the associated thermal wind account for the weakened WNPSH in the mid troposphere. We recommend the WNPSH be measured by eddy geopotential height (He) instead of traditionally used geopotential height, especially in climate change studies.
HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
Authors: Vivarini Áislan de Carvalho , Santos Pereira Renata de Meirelles , Barreto-de-Souza Victor , Temerozo Jairo Ramos , Soares Deivid C. , Saraiva Elvira M. , Saliba Alessandra Mattos , Bou-Habib Dumith Chequer , Lopes Ulisses Gazos
Published: November 2015

HIV-1 co-infection with human parasitic diseases is a growing public health problem worldwide. Leishmania parasites infect and replicate inside macrophages, thereby subverting host signaling pathways, including the response mediated by PKR. The HIV-1 Tat protein interacts with PKR and plays a pivotal role in HIV-1 replication. This study shows that Tat increases both the expression and activation of PKR in Leishmania-infected macrophages. Importantly, the positive effect of Tat addition on parasite growth was dependent on PKR signaling, as demonstrated in PKR-deficient macrophages or macrophages treated with the PKR inhibitor. The effect of HIV-1 Tat on parasite growth was prevented when the supernatant of HIV-1-infected macrophages was treated with neutralizing anti-HIV-1 Tat prior to Leishmania infection. The addition of HIV-1 Tat to Leishmania-infected macrophages led to inhibition of iNOS expression, modulation of NF-kB activation and enhancement of IL-10 expression. Accordingly, the expression of a Tat construct containing mutations in the basic region (49–57aa), which is responsible for the interaction with PKR, favored neither parasite growth nor IL-10 expression in infected macrophages. In summary, we show that Tat enhances Leishmania growth through PKR signaling.
Fibulin-4 deficiency increases TGF-β signalling in aortic smooth muscle cells due to elevated TGF-β2 levels
Authors: Ramnath N. W. M. , Hawinkels L. J. A. C. , van Heijningen P. M. , Riet L. te , Paauwe M. , Vermeij M. , Danser A. H. J. , Kanaar R. , ten Dijke P. , Essers J.
Published: November 2015

Fibulins are extracellular matrix proteins associated with elastic fibres. Homozygous Fibulin-4 mutations lead to life-threatening abnormalities such as aortic aneurysms. Aortic aneurysms in Fibulin-4 mutant mice were associated with upregulation of TGF-β signalling. How Fibulin-4 deficiency leads to deregulation of the TGF-β pathway is largely unknown. Isolated aortic smooth muscle cells (SMCs) from Fibulin-4 deficient mice showed reduced growth, which could be reversed by treatment with TGF-β neutralizing antibodies. In Fibulin-4 deficient SMCs increased TGF-β signalling was detected using a transcriptional reporter assay and by increased SMAD2 phosphorylation. Next, we investigated if the increased activity was due to increased levels of the three TGF-β isoforms. These data revealed slightly increased TGF-β1 and markedly increased TGF-β2 levels. Significantly increased TGF-β2 levels were also detectable in plasma from homozygous Fibulin-4R/R mice, not in wild type mice. TGF-β2 levels were reduced after losartan treatment, an angiotensin-II type-1 receptor blocker, known to prevent aortic aneurysm formation. In conclusion, we have shown increased TGF-β signalling in isolated SMCs from Fibulin-4 deficient mouse aortas, not only caused by increased levels of TGF-β1, but especially TGF-β2. These data provide new insights in the molecular interaction between Fibulin-4 and TGF-β pathway regulation in the pathogenesis of aortic aneurysms.
Toroidal qubits: naturally-decoupled quiet artificial atoms
Authors: Zagoskin Alexandre M. , Chipouline Arkadi , Il’ichev Evgeni , Johansson J. Robert , Nori Franco
Published: November 2015

The requirements of quantum computations impose high demands on the level of qubit protection from perturbations; in particular, from those produced by the environment. Here we propose a superconducting flux qubit design that is naturally protected from ambient noise. This decoupling is due to the qubit interacting with the electromagnetic field only through its toroidal moment, which provides an unusual qubit-field interaction, which is suppressed at low frequencies.
TAL effectors mediate high-efficiency transposition of the piggyBac transposon in silkworm Bombyx mori L
Authors: Ye Lupeng , You Zhengying , Qian Qiujie , Zhang Yuyu , Che Jiaqian , Song Jia , Zhong Boxiong
Published: November 2015

The piggyBac (PB) transposon is one of the most useful transposable elements and has been successfully used for genetic manipulation in more than a dozen species. However, the efficiency of PB-mediated transposition is still insufficient for many purposes. Here, we present a strategy to enhance transposition efficiency using a fusion of transcription activator-like effector (TALE) and the PB transposase (PBase). The results demonstrate that the TALE-PBase fusion protein which is engineered in this study can produce a significantly improved stable transposition efficiency of up to 63.9%, which is at least 7 times higher than the current transposition efficiency in silkworm. Moreover, the average number of transgene-positive individuals increased up to 5.7-fold, with each positive brood containing an average of 18.1 transgenic silkworms. Finally, we demonstrate that TALE-PBase fusion-mediated PB transposition presents a new insertional preference compared with original insertional preference. This method shows a great potential and value for insertional therapy of many genetic diseases. In conclusion, this new and powerful transposition technology will efficiently promote genetic manipulation studies in both invertebrates and vertebrates.
Analysis of volatile organic compounds in exhaled breath to diagnose ventilator-associated pneumonia
Authors: Schnabel Ronny , Fijten Rianne , Smolinska Agnieszka , Dallinga Jan , Boumans Marie-Louise , Stobberingh Ellen , Boots Agnes , Roekaerts Paul , Bergmans Dennis , van Schooten Frederik Jan
Published: November 2015

Ventilator-associated pneumonia (VAP) is a nosocomial infection occurring in the intensive care unit (ICU). The diagnostic standard is based on clinical criteria and bronchoalveolar lavage (BAL). Exhaled breath analysis is a promising non-invasive method for rapid diagnosis of diseases and contains volatile organic compounds (VOCs) that can differentiate diseased from healthy individuals. The aim of this study was to determine whether analysis of VOCs in exhaled breath can be used as a non-invasive monitoring tool for VAP. One hundred critically ill patients with clinical suspicion of VAP underwent BAL. Before BAL, exhaled air samples were collected and analysed by gas chromatography time-of-flight mass spectrometry (GC-tof-MS). The clinical suspicion of VAP was confirmed by BAL diagnostic criteria in 32 patients [VAP(+)] and rejected in 68 patients [VAP(−)]. Multivariate statistical comparison of VOC profiles between VAP(+) and VAP(−) revealed a subset of 12 VOCs that correctly discriminated between those two patient groups with a sensitivity and specificity of 75.8% ± 13.5% and 73.0% ± 11.8%, respectively. These results suggest that detection of VAP in ICU patients is possible by examining exhaled breath, enabling a simple, safe and non-invasive approach that could diminish diagnostic burden of VAP.
Intra- and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation
Authors: Wakamori Masatoshi , Fujii Yoshifumi , Suka Noriyuki , Shirouzu Mikako , Sakamoto Kensaku , Umehara Takashi , Yokoyama Shigeyuki
Published: November 2015

Post-translational modifications (PTMs) of histones, such as lysine acetylation of the N-terminal tails, play crucial roles in controlling gene expression. Due to the difficulty in reconstituting site-specifically acetylated nucleosomes with crystallization quality, structural analyses of histone acetylation are currently performed using synthesized tail peptides. Through engineering of the genetic code, translation termination and cell-free protein synthesis, we reconstituted human H4-mono- to tetra-acetylated nucleosome core particles (NCPs) and solved the crystal structures of the H4-K5/K8/K12/K16-tetra-acetylated NCP and unmodified NCP at 2.4 Å and 2.2 Å resolutions, respectively. The structure of the H4-tetra-acetylated NCP resembled that of the unmodified NCP and the DNA wrapped the histone octamer as precisely as in the unmodified NCP. However, the B-factors were significantly increased for the peripheral DNAs near the N-terminal tail of the intra- or inter-nucleosomal H4. In contrast, the B-factors were negligibly affected by the H4 tetra-acetylation in histone core residues, including those composing the acidic patch and at H4-R23, which interacts with the acidic patch of the neighboring NCP. The present study revealed that the H4 tetra-acetylation impairs NCP self-association by changing the interactions of the H4 tail with DNA and is the first demonstration of crystallization quality NCPs reconstituted with genuine PTMs.
Novel, low-cost solid-liquid-solid process for the synthesis of α-Si3N4 nanowires at lower temperatures and their luminescence properties
Authors: Liu Haitao , Huang Zhaohui , Huang Juntong , Fang Minghao , Liu Yan-gai , Wu Xiaowen , Hu Xiaozhi , Zhang Shaowei
Published: November 2015

Ultra-long, single crystal, α-Si3N4 nanowires sheathed with amorphous silicon oxide were synthesised by an improved, simplified solid-liquid-solid (SLS) method at 1150 °C without using flowing gases (N2, CH4, Ar, NH3, etc.). Phases, chemical composition and structural characterisation using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM/HRTEM), Fourier-transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) showed that the nanowires had Si3N4@SiOx core-shell structures. The growth of the nanowires was governed by the solid-liquid-solid (SLS) mechanism. The room temperature photoluminescence (PL) and cathodoluminescence (CL) spectra showed that the optical properties of the α-Si3N4 nanowires can be changed along with the excitation wavelength or the excitation light source. This work can be useful, not only for simplifying the design and synthesis of Si-related nanostructures, but also for developing new generation nanodevices with changeable photoelectronic properties.
Transparent Conductive Nanofiber Paper for Foldable Solar Cells
Authors: Nogi Masaya , Karakawa Makoto , Komoda Natsuki , Yagyu Hitomi , Nge Thi Thi
Published: November 2015

Optically transparent nanofiber paper containing silver nanowires showed high electrical conductivity and maintained the high transparency and low weight of the original transparent nanofiber paper. We demonstrated some procedures of optically transparent and electrically conductive cellulose nanofiber paper for lightweight and portable electronic devices. The nanofiber paper enhanced high conductivity without any post treatments such as heating or mechanical pressing, when cellulose nanofiber dispersions were dropped on a silver nanowire thin layer. The transparent conductive nanofiber paper showed high electrical durability in repeated folding tests, due to dual advantages of the hydrophilic affinity between cellulose and silver nanowires and the entanglement between cellulose nanofibers and silver nanowires. Their optical transparency and electrical conductivity were as high as those of ITO glass. Therefore, using this conductive transparent paper, organic solar cells were produced that achieved a power conversion of 3.2%, which was as high as that of ITO-based solar cells.
Magnetite-Amyloid-β deteriorates activity and functional organization in an in vitro model for Alzheimer’s disease
Authors: Teller Sara , Tahirbegi Islam Bogachan , Mir Mònica , Samitier Josep , Soriano Jordi
Published: November 2015

The understanding of the key mechanisms behind human brain deterioration in Alzheimer’ disease (AD) is a highly active field of research. The most widespread hypothesis considers a cascade of events initiated by amyloid-β peptide fibrils that ultimately lead to the formation of the lethal amyloid plaques. Recent studies have shown that other agents, in particular magnetite, can also play a pivotal role. To shed light on the action of magnetite and amyloid-β in the deterioration of neuronal circuits, we investigated their capacity to alter spontaneous activity patterns in cultured neuronal networks. Using a versatile experimental platform that allows the parallel monitoring of several cultures, the activity in controls was compared with the one in cultures dosed with magnetite, amyloid-β and magnetite-amyloid-β complex. A prominent degradation in spontaneous activity was observed solely when amyloid-β and magnetite acted together. Our work suggests that magnetite nanoparticles have a more prominent role in AD than previously thought and may bring new insights in the understanding of the damaging action of magnetite-amyloid-β complex. Our experimental system also offers new interesting perspectives to explore key biochemical players in neurological disorders through a controlled, model system manner.
Ultra High-Speed Radio Frequency Switch Based on Photonics
Authors: Ge Jia , Fok Mable P.
Published: November 2015

Microwave switches, or Radio Frequency (RF) switches have been intensively used in microwave systems for signal routing. Compared with the fast development of microwave and wireless systems, RF switches have been underdeveloped particularly in terms of switching speed and operating bandwidth. In this paper, we propose a photonics based RF switch that is capable of switching at tens of picoseconds speed, which is hundreds of times faster than any existing RF switch technologies. The high-speed switching property is achieved with the use of a rapidly tunable microwave photonic filter with tens of gigahertz frequency tuning speed, where the tuning mechanism is based on the ultra-fast electro-optics Pockels effect. The RF switch has a wide operation bandwidth of 12 GHz and can go up to 40 GHz, depending on the bandwidth of the modulator used in the scheme. The proposed RF switch can either work as an ON/OFF switch or a two-channel switch, tens of picoseconds switching speed is experimentally observed for both type of switches.
Synthetic pheromones and plant volatiles alter the expression of chemosensory genes in Spodoptera exigua
Authors: Wan Xinlong , Qian Kai , Du Yongjun
Published: November 2015

Pheromone and plant odorants are important for insect mating, foraging food sources and oviposition. To understand the molecular mechanisms regulating pheromone and odorant signaling, we employed qRT-PCR to study the circadian rhythms of ABP, OBP, PBP and OR gene expression in the beet armyworm, Spodoptera exigua and their responses after a pre-exposure to sex pheromone compounds or plant volatiles. The neuronal responses of male S. exigua to 20 chemical compounds were recorded at three specific time periods using the electroantennogram. The results showed a circadian rhythm in the expression profiles of some chemosensory genes in the antennae similar to their behavioral rhythm. The expression profiles of OR3, OR6, OR11, OR13, OR16, OR18, Orco, ABP2, OBP1, OBP7 and PBP1 and EAG responses to chemical compounds, as well as their circadian rhythm were significantly affected after exposure to synthetic sex pheromones and plant volatiles. These findings provide the first evidence that the gene expression of chemosensory genes and olfactory sensitivity to sex pheromones are affected by pre-exposing insects to pheromone compounds and plant volatiles. It helps to understand the molecular mechanisms underlying pheromone activity and the application of sex pheromones and plant volatiles in mating disruption or mass trapping.
Assembly of the MHC I peptide-loading complex determined by a conserved ionic lock-switch
Authors: Blees Andreas , Reichel Katrin , Trowitzsch Simon , Fisette Olivier , Bock Christoph , Abele Rupert , Hummer Gerhard , Schäfer Lars V. , Tampé Robert
Published: November 2015

Salt bridges in lipid bilayers play a decisive role in the dynamic assembly and downstream signaling of the natural killer and T-cell receptors. Here, we describe the identification of an inter-subunit salt bridge in the membrane within yet another key component of the immune system, the peptide-loading complex (PLC). The PLC regulates cell surface presentation of self-antigens and antigenic peptides via molecules of the major histocompatibility complex class I. We demonstrate that a single salt bridge in the membrane between the transporter associated with antigen processing TAP and the MHC I-specific chaperone tapasin is essential for the assembly of the PLC and for efficient MHC I antigen presentation. Molecular modeling and all-atom molecular dynamics simulations suggest an ionic lock-switch mechanism for the binding of TAP to tapasin, in which an unfavorable uncompensated charge in the ER-membrane is prevented through complex formation. Our findings not only deepen the understanding of the interaction network within the PLC, but also provide evidence for a general interaction principle of dynamic multiprotein membrane complexes in immunity.
Characterization of an Injury Induced Population of Muscle-Derived Stem Cell-Like Cells
Authors: Vojnits Kinga , Pan HaiYing , Mu Xiaodong , Li Yong
Published: November 2015

We recently discovered a novel population of stem cells from the injured murine skeletal muscle. These injury induced muscle-derived stem cell-like cells (iMuSCs) are partially reprogrammed from differentiated myogenic cells and display a pluripotent-like state. The iMuSCs exhibit stem cell properties including the ability to differentiate into multiple lineages, such as neurogenic and myogenic differentiations; they also display a superior migration capacity that demonstrating a strong ability of muscle engraftment in vivo. IMuSCs express several pluripotent and myogenic stem cell markers; have the capability to form embryoid bodies and teratomas and can differentiate into all three germ layers. Moreover, blastocyst microinjection showed that the iMuSCs contributed to chimeric embryos but could not complete germline transmission. Our results indicate that the iMuSCs are in a partially reprogrammed state of pluripotency, which are generated by the microenvironment of injured skeletal muscle.
Conservation and Role of Electrostatics in Thymidylate Synthase
Authors: Garg Divita , Skouloubris Stephane , Briffotaux Julien , Myllykallio Hannu , Wade Rebecca C.
Published: November 2015

Conservation of function across families of orthologous enzymes is generally accompanied by conservation of their active site electrostatic potentials. To study the electrostatic conservation in the highly conserved essential enzyme, thymidylate synthase (TS), we conducted a systematic species-based comparison of the electrostatic potential in the vicinity of its active site. Whereas the electrostatics of the active site of TS are generally well conserved, the TSs from minimal organisms do not conform to the overall trend. Since the genomes of minimal organisms have a high thymidine content compared to other organisms, the observation of non-conserved electrostatics was surprising. Analysis of the symbiotic relationship between minimal organisms and their hosts and the genetic completeness of the thymidine synthesis pathway suggested that TS from the minimal organismWigglesworthia glossinidia(W.g.b.) must be active. Four residues in the vicinity of the active site ofEscherichia coliTS were mutated individually and simultaneously to mimic the electrostatics of W.g.b TS. The measured activities of theE. coliTS mutants imply that conservation of electrostatics in the region of the active site is important for the activity of TS and suggest that the W.g.b. TS has the minimal activity necessary to support replication of its reduced genome.
The mechanism for microsporidian parasite suppression of the hindgut bacteria of the migratory locust Locusta migratoria manilensis
Authors: Tan Shu-qian , Zhang Kai-qi , Chen Hong-xing , Ge Yang , Ji Rong , Shi Wang-peng
Published: November 2015

Locusts aggregate into bands of nymphs and swarms of adults that can pose a major threat to crop. Previous studies have shown that infection by the microsporidian parasite Paranosema locustae prevents locust aggregation behavior and we show that gut bacteria, which produce components of locust aggregation pheromones, are substantially reduced in locusts infected with P. locustae. We found that P. locustae could reduce the diversity, abundance and community composition of Locusta migratoria’s gut bacteria. The parasite infection was also shown to interrupt the peroxidase activity of locust hindgut. Genome-wide expression analysis showed that the parasite infection suppressed peroxidase mRNA relative expression of locust hindgut, but had no effects on attacin expression and superoxide dismutase at 16 d post-inoculation with 20,000 P. locustae spores. Our findings reveal the mechanisms by which P. locustae impairs bacterial diversity and community structure of Locusta migratoria’s gut bacteria.