bluuebunny/crossref_metadata_2025 huggingface dataset:
Increasing Prevention of Mother to Child Transmission (PMTCT) Uptake through Facility-Based Health Promotion: Intervention Development
Authors: Elsheikh Ibrahim Elsiddig , Crutzen Rik , Adam Ishag , Abdelraheem Salah Ibrahim , Van den Borne Hubertus W.
Published: April 2023

In Sudan, the HIV testing rates during pregnancy remain low. Limitations in scaling and uptake of PMTCT services are linked to several factors within the healthcare system, including the motivation of healthcare providers. This article describes how a health facility-based health promotion intervention plan was developed, implemented, and evaluated to increase the uptake of PMTCT services using the Intervention Mapping approach. Individual-level and environmental determinants were previously identified and included in the intervention plan. Some factors that impacted the intention of women to test for HIV during pregnancy include level of knowledge on MTCT, who offers the HIV test, the fear and tension experienced when thinking about HIV/AIDS, the non-confidentiality of the HIV test results, and self-efficacy. This provides insights into how to develop, implement, and evaluate a facility-based health promotion intervention. The pre-assessment was critical in shaping the intervention and making it relevant and evidence based. The Intervention Mapping approach that was applied facilitated the systematic design of the intervention and supported guiding the implementation.
Perioperative Cetuximab with Cisplatin and 5-Fluorouracil in Esogastric Adenocarcinoma: A Phase II Study
Authors: Gronnier Caroline , Mariette Christophe , Lepage Come , Monterymard Carole , Jary Marine , Ferru Aurélie , Baconnier Mathieu , Adhoute Xavier , Tavan David , Perrier Hervé , Guerin-Meyer Véronique , Lecaille Cédric , Bonichon-Lamichhane Nathalie , Pillon Didier , Cojocarasu Oana , Egreteau Joëlle , D’journo Xavier Benoit , Dahan Laétitia , Locher Christophe , Texereau Patrick , Collet Denis , Michel Pierre , Ben Abdelghani Meher , Guimbaud Rosine , Muller Marie , Bouché Olivier , Piessen Guillaume
Published: April 2023

Purpose: While perioperative chemotherapy provides a survival benefit over surgery alone in gastric and gastroesophageal junction (G/GEJ) adenocarcinomas, the results need to be improved. This study aimed to evaluate the efficacy and safety of perioperative cetuximab combined with 5-fluorouracil and cisplatin. Patients and Methods: Patients received six cycles of cetuximab, cisplatin, and simplified LV5FU2 before and after surgery. The primary objective was a combined evaluation of the tumor objective response (TOR), assessed by computed tomography, and the absence of major toxicities resulting in discontinuation of neoadjuvant chemotherapy (NCT) (45% and 90%, respectively). Results: From 2011 to 2013, 65 patients were enrolled. From 64 patients evaluable for the primary endpoint, 19 (29.7%) had a morphological TOR and 61 (95.3%) did not stop NCT prematurely due to major toxicity. Sixty patients (92.3%) underwent resection. Sixteen patients (/56 available, 28.5%) had histological responses (Mandard tumor regression grade ≤3). After a median follow-up of 44.5 months, median disease-free and overall survival were 24.4 [95% CI: 16.4-39.4] and 40.3 months [95% CI: 27.5-NA], respectively. Conclusion: Adding cetuximab to the NCT regimen in operable G/GEJ adenocarcinomas is safe, but did not show enough efficacy in the present study to meet the primary endpoint (NCT01360086).
Highly Sensitive Detection of Heavy Metal Elements Using Laser-Induced Breakdown Spectroscopy Coupled with Chelating Resin Enrichment
Authors: Wang Jinmei , Li Gang , Zheng Peichao , Shata Sahar , Qazi Hafiz Imran Ahmad , Lu Jianshu , Liu Shaojian , Tian Hongwu , Dong Daming
Published: April 2023

In this study, we demonstrate a unique method for the detection of heavy metals such as chromium (Cr), copper (Cu), lead (Pb), and nickel (Ni) at trace levels in aqueous solutions via laser-induced breakdown spectroscopy (LIBS) enriched using chelating resin. Reduction in sample time preparation was shortened by tailoring the sample processing time with the aid of an enrichment method. The calibration curves and LODs of CH-90 chelating resin for enriching Cr, Cu, Pb, and Ni cations were established under the optimal conditions. The linear correlation coefficients were all above 0.98, and the detection limits were 0.148 mg/L, 0.150 mg/L, 0.149 mg/L, and 0.240 mg/L, corresponding to Cr, Cu, Pb, and Ni, respectively. The quantitative evaluation of the obtained results signifies that the proposed method is highly sensitive for detecting trace elements and offers a good correspondence of the acquired linear correlation with its calibration model. Results comparison with past studies suggests that the proposed method is able to achieve lower LODs for elements under investigation.
Micronutrient Deficiency in Children and Adolescents with Obesity—A Narrative Review
Authors: Calcaterra Valeria , Verduci Elvira , Milanta Chiara , Agostinelli Marta , Todisco Carolina Federica , Bona Federica , Dolor Jonabel , La Mendola Alice , Tosi Martina , Zuccotti Gianvincenzo
Published: April 2023

Childhood obesity represents a serious public health burden. Despite excessive dietary consumption, children with obesity present high rates of micronutrient deficiencies, such as deficiencies in minerals and specific vitamins; micronutrient deficiencies may have a pathogenic role in obesity-related metabolic comorbidities. In this narrative review, we analyzed the main deficiencies associated with obesity, their clinical consequences, and the evidence about a possible supplementation. Iron; vitamins A, B, C, D, and E; folic acid; zinc; and copper deficiencies represent the most common deficient microelements. The relationship between obesity and multiple micronutrient deficiencies remains unclear, and different mechanisms have been proposed. The medical care plan for pediatric obesity should include food choices with high nutritional content as part of a crucial approach to obesity-related complications. Unfortunately, only a few studies are available regarding the efficacy of oral supplementation or weight loss for treating them; thus, continuous nutritional monitoring is necessary.
Revisiting the COVID-19 Pandemic: An Insight into Long-Term Post-COVID Complications and Repurposing of Drugs
Authors: Majumder Rajib , Ghosh Sanmitra , Singh Manoj K. , Das Arpita , Roy Chowdhury Swagata , Saha Abinit , Saha Rudra P.
Published: April 2023

SARS-CoV-2 is a highly contagious and dangerous coronavirus that has been spreading around the world since late December 2019. Severe COVID-19 has been observed to induce severe damage to the alveoli, and the slow loss of lung function led to the deaths of many patients. Scientists from all over the world are now saying that SARS-CoV-2 can spread through the air, which is a very frightening prospect for humans. Many scientists thought that this virus would evolve during the first wave of the pandemic and that the second wave of reinfection with the coronavirus would also be very dangerous. In late 2020 and early 2021, researchers found different genetic versions of the SARS-CoV-2 virus in many places around the world. Patients with different types of viruses had different symptoms. It is now evident from numerous case studies that many COVID-19 patients who are released from nursing homes or hospitals are more prone to developing multi-organ dysfunction than the general population. Understanding the pathophysiology of COVID-19 and its impact on various organ systems is crucial for developing effective treatment strategies and managing long-term health consequences. The case studies highlighted in this review provide valuable insights into the ongoing health concerns of individuals affected by COVID-19.
Morphology and Structure Characteristics of the Rare Black-Glazed Porcelains Excavated from the Jian Kiln Site of Song Dynasty
Authors: Tao Shiqian , Zhu Yuguang , Liu Song , Dong Junqing , Yuan Yimeng , Li Qinghui
Published: April 2023

Jian tea bowls are greatly appreciated not only due to their thick and lustrous black glazes, but also their exquisite streaked and mottled patterns. Researches on the firing process and colouring mechanism of typical hare’s fur and oil spot Jian glazes have continuously been carried out in the past decades; however, there are few reports about other scarce types of black-glazed porcelains excavated from the Jian kiln site. In this work, we report on a multi-technique analysis of the surface and cross-sectional structure, phase, morphology and chemical composition of three rare types of Jian tea bowls (tea-dust-glazed ware, grey-glazed ware and pearl-glazed ware) combining optical and spectroscopic methods such as optical coherence tomography (OCT), optical microscopy (OM), Portable energy dispersive X-ray fluorescence spectrometry (XRF), laser-Raman spectroscopy (LRS) and scanning electron microscopy coupled with an X-ray energy dispersive spectrometer (SEM-EDS). Results revealed that the tea dust glaze was one high-temperature iron-based crystalline glaze, and its glaze was characterized by a transparent glass matrix; irregular pits completely or partially filled by TiO2; and that ε-Fe2O3 and residual quartz were randomly distributed all over the surface, which produced the appearance of tea-dust. The grey-glazed porcelain was fired at a lower temperature in the dragon kiln than that of the traditional hare’s fur and oil spot samples, its glaze contained some residual quartz, and anorthite crystals showed a serried distribution along the thickness. The pearl-glazed porcelain was fired through a secondary glazing process via artificial stippling quartz powder. This work provides guidance for the firing process of three rare types of Jian wares and has not only scientific but also cultural profound significance, which reveals the features of their historical heritage.
Enhanced Photocatalytic Activities of Ag2WO4 Modified Ag6Si2O7 through a Comprehensive p-n Heterojunction S-Scheme Process
Authors: Shah Navid , Li Mengke , Zhang Ping , Cui Yanyan , Wang Yaling
Published: April 2023

The S-scheme photocatalyst system has become increasingly popular in recent years for its ability to efficiently degrade various pollutants, including organic dyes, pesticides, and other harmful substances. This system uses two semiconductor photocatalysts with different bandgap energies, working together in a redox reaction to produce a highly reactive species capable of pollutant breakdown. Here, an S-scheme Ag2WO4/Ag6Si2O7 p-n heterojunction nanocomposite was successfully developed by a coprecipitation method. By decomposing Rhodamine B (RhB) under visible-light irradiation, the photocatalytic activities of Ag6Si2O7/Ag2WO4 showed enhanced photocatalytic degradation performance of organic dyes, especially at a 4% molar ratio of the Ag2WO4-modified Ag6Si2O7 sample, whose degradation rate was 23.7 and 4.65 times those of Ag2WO4 and Ag6Si2O7, respectively. The physical and chemical properties of the samples were determined by identifying the physical structure, chemical element composition, and optical responsiveness. The optimum composite amongst the prepared materials was AgSW-4, achieving the maximum RhB degradation efficiency of 97.5%, which was higher by 60% and 20% than its counterparts Ag6Si2O7 and Ag2WO4, respectively. These results showed that in the nanocomposite structure, Ag6Si2O7 was a p-type semiconductor and Ag2WO4 was an n-type semiconductor. Based on the analysis data, a comprehensive p-n heterojunction S-scheme process was proposed to demonstrate the enhanced photocatalytic performance of the Ag6Si2O7/Ag2WO4 nanocomposite.
Fungal Diversity Associated with Armadillidium Isopods: A Case Study in Central Park of Gwacheon, South Korea
Authors: Cha Yehyeon , Oh Seung-Yoon
Published: April 2023

The symbiotic relationship between macroorganisms, such as plants and animals, and the microorganisms in their environment plays a crucial role in shaping their physiology and ecology. Thus, many studies have examined microbial symbiosis in relation to plants, humans, and insects. However, little is known about the microbial diversity associated with isopods. Hence, in this study, we investigated the fungal diversity associated with two species of terrestrial isopods, Armadillidium nasatum and A. vulgare. In this study, we used a metabarcoding approach to compare fungal diversity between the two species for the first time. Our results indicated that A. nasatum had significantly greater mycobiome alpha diversity than A. vulgare. In contrast, fungal communities (beta diversity) did not differ significantly between hosts, except in beta dispersion of relative abundance. The majority of fungi identified belonged to Ascomycota and Basidiomycota, phyla that are dominated by saprotrophs. In conclusion, our findings shed light on the fungal communities associated with Armadillidium species, providing valuable insight into the biology of terrestrial isopods.
Stenomitos nagquensis sp. nov. (Leptolyngbyaceae, Cyanobacteria) from a Meadow Wetland in the Tibet Plateau: A Novel Species Studied Based on a Polyphasic Approach
Authors: Pecundo Melissa H. , Chen Tao , Wang Yunhua , Wen Xuemei , Hu Zhangli , Chen Huirong , Li Nan
Published: April 2023

A novel cyanobacterial strain (CSML-F035) here described as Stenomitos nagquensis sp. nov. was isolated from a plateau meadow wetland habitat in the Tibet Autonomous Region. The novel strain was studied based on a polyphasic approach by combining characteristics of cell morphology and evidence from molecular data and ecology. Phylogenetic analyses based on the 16S rRNA and rbcL genes indicated that the novel strain is a member of the genus Stenomitos (Leptolyngbyaceae) but is positioned in a unique clade, with strongly supported values from BI, ML, and NJ methods. The percentage similarity of the 16S rRNA gene sequence of CSML-F035 compared with known strains of Stenomitos was >97%. Morphologically, S. nagquensis sp. nov. possesses overlapping morphological characteristics with other Stenomitos species, with thin trichomes enclosed in a firm, colorless, hyaline, persistent mucilage. However, the strain appeared to have slightly smaller cells compared to most known species of Stenomitos. Further analyses of the 16S–23S ITS region showed dissimilarity at 11.2–22.8% compared to known species of Stenomitos. The secondary helices—including D1–D1, V2, Box–B, and V3—that were predicted based on the 16S–23S ITS region for Stenomitos nagquensis sp. nov. were also distinct, thereby providing strong additional support for the separation of this species. This study highlights the importance of unexplored habitats in the Tibet region in which cyanobacterial diversity can be deemed high, with many potential new species that remain poorly explored.
By Visualizing the Deformation with Mechanoluminescent Particles, Additive Manufacturing Offers a Practical Alternative to Stress and Strain Simulation
Authors: Einbergs Ernests , Spustaka Agnese , Vitola Virginija , Zolotarjovs Aleksejs
Published: April 2023

The use of stress–strain analysis in structural design or mechanical components is critical for avoiding or investigating structural failures. In the case of complicated designs, mathematical full-field stress modeling produces imprecise predictions. Experimental analysis can be used as a replacement for mathematical modeling, but with the use of currently available strain gauges, it is cumbersome and impossible in the case of moving parts. Mechanoluminescent materials transform mechanical energy into visible light and can be used as a replacement for strain gauges to monitor strain/stress. Three-dimensional printing technology has made major advances in terms of additive manufacturing. In this article, we describe a method to produce an ML 3D print. The fabricated samples are precise and versatile and satisfy the need for easy and non-destructible spatial stress analysis. A 3D printed photopolymer sample with SrAl2O4: Eu, Dy particle addition only to the final layers was tested, and the number of layers was optimized. It was determined that the optimal number of layers for easy detection is in the range of 10 to 20 layers. It opens the possibility for the real-time evaluation of complex uneven forces on complex parts, thus having a good potential for commercialization.
Isovolumetric Contraction as a Marker of Ventricular Performance in Patients with Afterload Mismatch
Authors: Sinanis Theodoros , Markidis Eleftherios , Mavroudeas Symeon Evangelos , Sideras Emmanouil , Vittorakis Evangelos , Vittorakis Eftychios
Published: April 2023

Introduction: The evaluation of myocardial contractility is essential in cardiology practice. The gold standard for this evaluation is the end-systolic elastance, but it the method involved is complex. Echocardiographic measurement of the ejection fraction (EF) is the most commonly used parameter in clinical practice, but it has significant limitations, especially in patients with afterload mismatch. In this study, the area under the curve (AUC) of the isovolumetric contraction was measured to evaluate the myocardial contractility in patients with pulmonary arterial hypertension and severe aortic stenosis. Methods: 110 patients with severe aortic stenosis and pulmonary arterial hypertension were included in this study. The AUC of the isovolumetric contraction was measured using pressure curves of the right ventricle–pulmonary artery and left ventricle–aorta ascendens. This AUC was then correlated with the echocardiographically measured EF, stroke volume (SV), and total ventricular work. Results: The AUC of the isovolumetric contraction showed a statistically significant correlation with the EF of the corresponding ventricle (p < 0.0001). Both the AUC of the isovolumetric contraction and the EF showed a statistically significant correlation with the total work of the ventricle (AUC: R2 0.49, p < 0.001; EF: R2 0.51, p < 0.001). However, the SV only showed a statistically significant correlation with the EF. A statistically significant one-sample t-test could be found for the EF (decreased, p < 0.001) and for the AUC of the isovolumetric contraction (increased, p < 0.001), but not for the total work of the ventricle. Conclusion: The AUC space of the isovolumetric contraction is a useful marker of ventricular performance in patients with afterload mismatch, showing a statistically significant correlation with the EF and the total ventricular work. This method may have potential for use in clinical practice, especially in challenging cardiological cases. However, further studies are needed to evaluate its usefulness in healthy individuals and in other clinical scenarios.
Review on the Role of Salivary Biomarkers in the Diagnosis of Mild Traumatic Brain Injury and Post-Concussion Syndrome
Authors: Mavroudis Ioannis , Petridis Foivos , Balmus Ioana-Miruna , Ciobica Alin , Gorgan Dragos Lucian , Luca Alina Costina
Published: April 2023

(1) Background: While mild traumatic brain injuries (TBIs) are a major public health issue, post-concussion syndrome (PCS) remains a controversial entity. In both cases, the clinical diagnosis is mainly based on the symptoms and brain imaging evaluation. The current molecular biomarkers were described from blood and cerebrospinal fluid (CSF), yet both fluid collection methods are invasive. Saliva could be preferred in molecular diagnosis due to its non-invasive and non-expensive methods of acquisition, transport, and samples processing. (2) Objectives: In the present study, we aimed to review the latest developments in salivary biomarkers and their potential role in diagnosing mild TBIs, and PCS. (3) Results: In TBIs and PCS, a few novel studies focusing on salivary biomarkers have emphasized their importance in diagnosis. The previous studies mainly focused on micro RNAs, and only a few on extracellular vesicles, neurofilament light chain, and S100B. (4) Conclusions: The combination between salivary biomarkers, clinical history and examination, self-reported symptoms, and cognitive/balance testing can provide a non-invasive alternative diagnostic methodology, as compared to the currently approved plasma and cerebrospinal fluid biomarkers.
Health Status Evaluation of Welding Robots Based on the Evidential Reasoning Rule
Authors: Zhang Bang-Cheng , Wang Ji-Dong , Gao Shuo , Yin Xiao-Jing , Gao Zhi
Published: April 2023

It is extremely important to monitor the health status of welding robots for the safe and stable operation of a body-in-white (BIW) welding production line. In the actual production process, the robot degradation rate is slow and the effective data are poor, which can reflect a degradation state in the large amount of obtained monitoring data, which causes difficulties in health status evaluation. In order to realize the accurate evaluation of the health status of welding robots, this paper proposes a health status evaluation method based on the evidential reasoning (ER) rule, which reflects the health status of welding robots by using the running state data monitored in actual engineering and through the qualitative knowledge of experts, which makes up for the lack of effective data. In the ER rule evaluation model, the covariance matrix adaptive evolutionary strategy (CMA-ES) algorithm is used to optimize the initial parameters of the evaluation model, which improved the accuracy of health status evaluations. Finally, a BIW welding robot was taken as an example for verification. The results show that the proposed model is able to accurately estimate the health status of the welding robot by using the monitored degradation data.
In Situ Biogas Upgrading in a Randomly Packed Gas-Stirred Tank Reactor (GSTR)
Authors: Lembo Giuseppe , Rosa Silvia , Marone Antonella , Signorini Antonella
Published: April 2023

This study evaluated different strategies to increase gas–liquid mass transfer in a randomly packed gas stirred tank reactor (GSTR) continuously fed with second cheese whey (SCW), at thermophilic condition (55 °C), for the purpose of carrying out in situ biogas upgrading. Two different H2 addition rates (1.18 and 1.47 LH2 LR−1 d−1) and three different biogas recirculation rates (118, 176 and 235 L LR−1 d−1) were applied. The higher recirculation rate showed the best upgrading performance; H2 utilization efficiency averaged 88%, and the CH4 concentration in biogas increased from 49.3% during conventional anaerobic digestion to 75%, with a methane evolution rate of 0.37 LCH4 LR−1 d−1. The microbial community samples were collected at the end of each experimental phase, as well as one of the thermophilic sludge used as inoculum; metanogenomic analysis was performed using Illumina-based 16S sequencing. The whole microbial community composition was kept quite stable throughout the conventional anaerobic digestion (AD) and during the H2 addition experimental phases (UP1, UP2, UP3, UP4). On the contrary, the methanogens community was deeply modified by the addition of H2 to the GSTR. Methanogens of the Methanoculleus genus progressively increased in UP1 (47%) and UP2 (51%) until they became dominant in UP3 (94%) and UP4 (77%). At the same time, members of Methanotermobacter genus decreased to 19%, 23%, 3% and 10% in UP1, UP2, UP3 and UP4, respectively. In addition, members of the Methanosarcina genus decreased during the hydrogen addition phases.
An Assessment of Energy Flexibility Solutions from the Perspective of Low-Tech
Authors: Shahid Muhammad Salman , Osonuga Seun , Twum-Duah Nana Kofi , Hodencq Sacha , Delinchant Benoit , Wurtz Frédéric
Published: April 2023

The energy transition is a multidisciplinary challenge that warrants solutions that are robust and sustainable. Energy flexibility, one of the key pillars of the energy transition, is an umbrella term that covers multiple innovative solutions implemented at all levels of the electric grid to ensure power quality standards, amongst other objectives. Low-tech, on the other hand, emphasizes designing, producing, and sustainably implementing solutions. Therefore, considering the multidisciplinary nature of energy transition and the existing energy flexibility solutions, the purpose of this research work is multilateral: first, it presents the concept of low-tech and its associated mechanisms; then, it addresses the misconceptions and similarities that low-tech might have with other innovation approaches; and finally, it provides an assessment of existing flexibility solutions using low-tech as a tool. The result of this assessment is presented qualitatively and indicates that indirect energy flexibility solutions rank higher on a low-tech scale relative to supply-side energy flexibility solutions and energy storage flexibility solutions.
Analytical Modeling of Magnetic Air-Gap Field Distribution Due to Armature Reaction
Authors: Di Gerlando Antonino , Ricca Claudio
Published: April 2023

The following paper presents an analytical study of the air-gap magnetic field distribution produced by the armature reaction of a linear machine. Based on the method of images, the magnetic field generated by a current carrying conductor inside the air-gap between two smooth infinitely permeable iron surfaces is modeled as a complex 2D function. The conductor model then becomes a current sheet model and horizontally oriented current sheets are used to model the magnetic field produced by the armature reaction for smooth ferromagnetic surfaces. Focus will be given to the study of the slot opening function in front of energized slots in comparison to the not energized ones of the classical theory pointing out some remarkable differences. Later, the model is extended to slotted geometries using a complex slotting function adapted for energized slots. At last, the Maxwell tensor expressed in complex formulation will be integrated to obtain the force components acting on the machine tooth tips, quantities that will be compared with FEM simulations in order to validate the proposed analytical model.
Effect of Recycling on the Environmental Impact of a High-Efficiency Photovoltaic Module Combining Space-Grade Solar Cells and Optical Micro-Tracking
Authors: Ziemińska-Stolarska Aleksandra , Pietrzak Monika , Zbiciński Ireneusz
Published: April 2023

This paper presents a life cycle assessment (LCA) analysis of a new, high-concentration photovoltaic (HCPV) technology developed as part of the HIPERION project of hybrid photovoltaics for efficiency record using an integrated optical technology. In the LCA calculations, the production stage of a full module was adopted as a functional unit. SimaPro version 9.00.49, the recent Ecoinvent database (3.8), and the IPCC 2021 GWP 100a environmental model were applied to perform the calculations. The environmental impact of the HCPV panel was determined for constructional data and for recycling of the main elements of the module. The results of the calculations show that recycling of PMMA, rubber, and electronic elements reduced the total carbon footprint by 17%, from 240 to 201 kg CO2-eq. The biggest environmental load was generated by the PV cells: 99.9 kg CO2eq., which corresponds to 49.8% (41.7% without recycling) of the total environmental load due to the large number of solar cells used in the construction. The emission of CO2 over a 25-year lifespan was determined from 17.1 to 23.4 g CO2-eq/kWh (20.4 to 27.9 without recycling), depending on the location. The energy payback time (EPBT) for the analyzed module is 0.87 and 1.19 years, depending on the location and the related insolation factors (Madrid: 470 kWh/m2, Lyon: 344 kWh/m2). The results of the calculations proved that the application of recycling and recovery methods for solar cells can improve the sustainability of the photovoltaic industry.
Response Mechanism of Leaf Area Index and Main Nutrient Content in Mangrove Supported by Hyperspectral Data
Authors: Chen Xiaohua , Yang Yuechao , Zhang Donghui , Li Xusheng , Gao Yu , Zhang Lifu , Wang Daming , Wang Jianhua , Wang Jin , Huang Jin
Published: April 2023

Mangrove is the key vegetation in the transitional zone between land and sea, and its health assessment can indicate the deep-level ecological information. The LAI and six key nutrients of mangrove were selected as quantitative evaluation indicators, and the decisive evaluation method of mangrove growth was expected. The mangrove reserve of Dongzhai Port National Nature Reserve in Hainan Province, China, was selected as the study area, with an area of 17.71 km2. The study area was divided into adjacent urban areas, aquaculture areas, and agricultural production areas, and key indicators are extracted from satellite hyperspectral data. The extraction process includes spectral data preprocessing, spectral transformation, spectral combination, spectral modeling, and precision inspection. The spatial distribution of LAI and six key nutrient components of mangrove in the study area were obtained. LAI and Chla need to calculate the index after high-order differentiation of the spectrum; MSTR and Chlb need to calculate the envelope after the second-order differential of the spectrum; TN and TP are directly changed by original or exponential spectrum; the spectral transformation method adopted by TK was homogenization after first-order differential. The results of the strength of nutrient content along the three regions show that there was no significant difference in the retrieval index of mangroves in the three regions, and the overall health level of mangroves was consistent. Chla was the key identification component of mangrove growth and health. The contents of nutrient elements with correlation coefficient exceeding 0.80 include MSTR and TK (0.98), Chla and TP (0.96), Chla and TK (0.87), MSTR and Chla (0.86), MSTR and TK (0.83), and MSTR and TP (0.81). The study quantifies the relationship between different LAI and nutrient content of mangrove leaves from the perspectives of water, leaf biology, and chemical elements, which improved our understanding of the relationship between key components during mangrove growth for the first time.
New Observation in Biocontrol of Penicillium caperatum against Fusarium oxysporum on Saposhnikovia divaricata and as a Plant Growth Promoter
Authors: Han Zhongming , Wang Jiao , Ding Yanzhe , Sun Zhuo , Wang Yan , Wang Yuyi , Yang Limin , Wang Yunhe
Published: April 2023

Fusarium oxysporum, a common fungal pathogen that infects economic crops, causes Fusarium wilt disease to Saposhnikovia divaricata at an annual incidence rate of more than 15%. This study aimed to assess the potential of rhizospheric fungi as antifungal agents against Fusarium wilt of Saposhnikovia divaricata. In this study, 104 fungi were isolated from S. divaricata rhizospheric soil. Twelve rhizospheric strains that showed antagonistic activity against F. oxysporum, MR-16, MR-32, MR-38, etc., were screened out. Biocontrol activities of the twelve strains, especially MR-16, were subsequently characterized and evaluated. Strain MR-16 as potential stock for biocontrol had good antibiotic activity against F. oxysporum in vitro experiment. Based on the analysis of morphological properties and rDNA internal transcribed spacers (ITS), we identified an isolate MR-16 as Penicillium caperatum (GenBank No. OK287146.1), a new record of this species of China. The results of the in vitro antagonistic assay indicated that the conidial germination rate was significantly decreased, and the mycelia morphology of F. oxysporum induced change via the culture filtrate of P. caperatum MR-16, such as deformation and degradation. In an outdoor pot experiment, inoculation of S. divaricata plants with F. oxysporum created severe wilting symptoms; however, in inoculation trials, MR-16 effectively suppressed disease lesions, with a strong control efficacy of 60.76%. In addition, strain MR-16 could successfully colonize and form stable populations in the soil, and it showed a continuous positive growth-promoting effect on S. divaricata plants.
Epicatechin Inhibited Lipid Oxidation and Protein Lipoxidation in a Fish Oil-Fortified Dairy Mimicking System
Authors: Lian Zhenghao , Han Jiahui , Cao Yue , Yao Wenhua , Niu Xiaoying , Xu Mingfeng , Xu Jun , Zhu Qin
Published: April 2023

In this study, a typical tea polyphenol epicatechin (EC) was investigated for its impact on the oxidative stability of whey protein isolate (WPI) in a fish oil-fortified emulsion. The oil-in-water emulsion system consisted of fish oil (1%, w/w), WPI (6 mg/mL), and EC (0.1, 1, and 2 mM), and the oxidation reaction was catalyzed by Fenton’s reagent at 25 °C for 24 h. The results showed EC exhibited a dose-dependent activity in the reduction of lipid oxidation (TBARS) and protein carbonylation. A Western blot analysis demonstrated that protein lipoxidation was inhibited by EC via interrupting the covalent binding of lipid secondary oxidation products, MDA, onto proteins. In addition, protein lipoxidation induced a loss of tryptophan fluorescence, and protein hydrolysis was partially recovered by EC. The findings of this study provide an in-depth understanding of the performance of phenolic antioxidants in relieving lipid oxidation and subsequent protein lipoxidation in oil-containing dairy products.
Chromosome-Level Genome Assembly of the Blue Mussel Mytilus chilensis Reveals Molecular Signatures Facing the Marine Environment
Authors: Gallardo-Escárate Cristian , Valenzuela-Muñoz Valentina , Nuñez-Acuña Gustavo , Valenzuela-Miranda Diego , Tapia Fabian J. , Yévenes Marco , Gajardo Gonzalo , Toro Jorge E. , Oyarzún Pablo A. , Arriagada Gloria , Novoa Beatriz , Figueras Antonio , Roberts Steven , Gerdol Marco
Published: April 2023

The blue mussel Mytilus chilensis is an endemic and key socioeconomic species inhabiting the southern coast of Chile. This bivalve species supports a booming aquaculture industry, which entirely relies on artificially collected seeds from natural beds that are translocated to diverse physical–chemical ocean farming conditions. Furthermore, mussel production is threatened by a broad range of microorganisms, pollution, and environmental stressors that eventually impact its survival and growth. Herein, understanding the genomic basis of the local adaption is pivotal to developing sustainable shellfish aquaculture. We present a high-quality reference genome of M. chilensis, which is the first chromosome-level genome for a Mytilidae member in South America. The assembled genome size was 1.93 Gb, with a contig N50 of 134 Mb. Through Hi-C proximity ligation, 11,868 contigs were clustered, ordered, and assembled into 14 chromosomes in congruence with the karyological evidence. The M. chilensis genome comprises 34,530 genes and 4795 non-coding RNAs. A total of 57% of the genome contains repetitive sequences with predominancy of LTR-retrotransposons and unknown elements. Comparative genome analysis of M. chilensis and M. coruscus was conducted, revealing genic rearrangements distributed into the whole genome. Notably, transposable Steamer-like elements associated with horizontal transmissible cancer were explored in reference genomes, suggesting putative relationships at the chromosome level in Bivalvia. Genome expression analysis was also conducted, showing putative genomic differences between two ecologically different mussel populations. The evidence suggests that local genome adaptation and physiological plasticity can be analyzed to develop sustainable mussel production. The genome of M. chilensis provides pivotal molecular knowledge for the Mytilus complex.
Ethanol and Methyl Jasmonate Fumigation Impact on Quality, Antioxidant Capacity, and Phytochemical Content of Broccoli Florets during Storage
Authors: Duarte-Sierra Arturo , Thomas Minty , Arul Joseph
Published: April 2023

Broccoli is a vegetable that offers valuable components, such as glucosinolates (GLS), flavonoids, and hydroxycinnamic acids (HCA), for our daily food intake. These substances have been associated with reducing the risk of cancer and cardiovascular diseases (CVD). Broccoli florets are also highly perishable, given their elevated respiration rates and their sensitivity to ethylene. Experiments have been carried out on broccoli to investigate the consequences of abiotic stress post-harvest treatments in retarding the senescence process. Nevertheless, the influence of these treatments on the phytochemicals of broccoli has not been extensively examined. Florets of broccoli (Brassica oleracea) were exposed to an atmosphere consisting of 10,000 µL.L−1 ethanol at room temperature for 30 min and 120 min. The exposure to methyl jasmonate (MeJA) treatments was carried out at room temperature using 1 µL.L−1 for 45 min and 180 min. The yellowing of florets was delayed using 10,000 µL.L−1 of ethanol at both exposure times as compared to untreated florets, and the chlorophyll titers were also superior with both doses over the control. The total phenols of the florets increased by 15% and 18% with the application of the hormetic and high doses, respectively, throughout the storage period compared to unexposed broccoli. The GLS and HCA yields were also increased by both ethanol doses. The exposure of florets to 1 µL.L1 MeJA for 45 min resulted in delayed yellowing of florets; however, longer exposures resulted in yellowing after 21 d and significantly (p < 0.05) increased respiration rates relative to untreated florets. Overall antioxidant capacity of the florets was significantly reduced with both doses of methyl jasmonate; however, HCA titers were increased at both doses. The amount of total glucobrassicins within broccoli was increased following exposure of florets to both doses, but no significant differences in glucoraphanin content were observed. As a conclusion, the ethanol treatment could indeed delay senescence and lead to the induction of phytochemicals. In contrast, MeJA’s effect on quality is not quite substantial; it can, however, be used to improve the phytochemical content of florets, particularly indole-type GLS.
Role of Maternal Diet in the Risk of Childhood Acute Leukemia: A Systematic Review and Meta-Analysis
Authors: Blanco-Lopez Jessica , Iguacel Isabel , Pisanu Silvia , Almeida Claudia , Steliarova-Foucher Eva , Sierens Ciska , Gunter Marc , Ladas Elena , Barr Ronald , Van Herck Koen , Huybrechts Inge
Published: April 2023

Many studies have investigated the etiology of acute leukemia, one of the most common types of cancer in children; however, there is a lack of clarity regarding preventable risk factors. This systematic review and meta-analysis aimed to summarize the current evidence regarding the role of maternal dietary factors in the development of childhood leukemia. All epidemiological studies published until July 2022 that evaluated maternal dietary risk factors for childhood acute leukemia were identified in two electronic databases (PubMed and Web of Science) without limits of publication year or language. A total of 36 studies (1 prospective cohort study, 32 case-control studies and 3 studies with pooled analysis) were included. The published risk estimates were combined into a meta-analysis, using the Generic Inverse Variance method. The maternal consumption of fruits (two or more daily servings vs. less) was inversely associated with acute lymphoblastic leukemia (odds ratio = 0.71; 95% CI, 0.59–0.86), whereas maternal coffee intake (higher than two cups per day vs. no consumption) was associated with an increased risk of acute lymphoblastic leukemia (odds ratio = 1.45; 95% CI, 1.12–1.89). Despite these findings, more high-quality research from cohort studies and the identification of causal factors are needed to develop evidence-based and cost-effective prevention strategies applicable at the population level. Review Registration: PROSPERO registration no. CRD42019128937.
Germination and Growth of Plasma-Treated Maize Seeds Planted in Fields and Exposed to Realistic Environmental Conditions
Authors: Recek Nina , Zaplotnik Rok , Vesel Alenka , Primc Gregor , Gselman Peter , Mozetič Miran , Holc Matej
Published: April 2023

In this study, we applied an inductively coupled, radio frequency oxygen plasma to maize seeds and investigated its effects on seedling emergence, plant number at tasseling, and crop yield of maize in realistic field conditions. Maize seeds of seven different hybrids were treated over two harvest years. In addition to plasma-treated seeds, a control sample, fungicide-treated seeds, an eco-layer, and a plasma and eco-layer combination, were planted. Seedling emergence, plant number at tasseling (plants/m2), and yield (kg/ha), were recorded. In the first harvest year, results were negatively affected by the presence of an insect pest. In the second harvest year, plant number and yield results were more uniform. In both years, for two and three hybrids, respectively, the highest yield arose from plants from plasma-treated seeds, but the differences were only partially significant. Considering our results, plasma treatment of maize seeds appears to have a positive effect on the yield of the plant.
The Role of Tyr-His-Trp Triad and Water Molecule Near the N1-Atom of 2-Hydroperoxycoelenterazine in Bioluminescence of Hydromedusan Photoproteins: Structural and Mutagenesis Study
Authors: Natashin Pavel V. , Burakova Ludmila P. , Kovaleva Margarita I. , Shevtsov Mikhail B. , Dmitrieva Daria A. , Eremeeva Elena V. , Markova Svetlana V. , Mishin Alexey V. , Borshchevskiy Valentin I. , Vysotski Eugene S.
Published: April 2023

Hydromedusan photoproteins responsible for the bioluminescence of a variety of marine jellyfish and hydroids are a unique biochemical system recognized as a stable enzyme-substrate complex consisting of apoprotein and preoxygenated coelenterazine, which is tightly bound in the protein inner cavity. The binding of calcium ions to the photoprotein molecule is only required to initiate the light emission reaction. Although numerous experimental and theoretical studies on the bioluminescence of these photoproteins were performed, many features of their functioning are yet unclear. In particular, which ionic state of dioxetanone intermediate decomposes to yield a coelenteramide in an excited state and the role of the water molecule residing in a proximity to the N1 atom of 2-hydroperoxycoelenterazine in the bioluminescence reaction are still under discussion. With the aim to elucidate the function of this water molecule as well as to pinpoint the amino acid residues presumably involved in the protonation of the primarily formed dioxetanone anion, we constructed a set of single and double obelin and aequorin mutants with substitutions of His, Trp, Tyr, and Ser to residues with different properties of side chains and investigated their bioluminescence properties (specific activity, bioluminescence spectra, stopped-flow kinetics, and fluorescence spectra of Ca2+-discharged photoproteins). Moreover, we determined the spatial structure of the obelin mutant with a substitution of His64, the key residue of the presumable proton transfer, to Phe. On the ground of the bioluminescence properties of the obelin and aequorin mutants as well as the spatial structures of the obelin mutants with the replacements of His64 and Tyr138, the conclusion was made that, in fact, His residue of the Tyr-His-Trp triad and the water molecule perform the “catalytic function” by transferring the proton from solvent to the dioxetanone anion to generate its neutral ionic state in complex with water, as only the decomposition of this form of dioxetanone can provide the highest light output in the light-emitting reaction of the hydromedusan photoproteins.
Nonsteroidal Anti-Inflammatory Drug Conjugated with Gadolinium (III) Complex as an Anti-Inflammatory MRI Agent
Authors: Sung Bokyung , Kim Hee-Kyung , Baek Ah-Rum , Yang Byeong-Woo , Kim Yeoun-Hee , Choi Garam , Park Hyun-Jin , Kim Minsup , Lee Jongmin , Chang Yongmin
Published: April 2023

Studies have been actively conducted to ensure that gadolinium-based contrast agents for magnetic resonance imaging (MRI) are accompanied by various biological functions. A new example is the anti-inflammatory theragnostic MRI agent to target inflammatory mediators for imaging diagnosis and to treat inflammatory diseases simultaneously. We designed, synthesized, and characterized a Gd complex of 1,4,7-tris(carboxymethylaza) cyclododecane-10-azaacetylamide (DO3A) conjugated with a nonsteroidal anti-inflammatory drug (NSAID) that exerts the innate therapeutic effect of NSAIDs and is also applicable in MRI diagnostics. Gd-DO3A-fen (0.1 mmol/kg) was intravenously injected into the turpentine oil-induced mouse model, with Gd-DO3A-BT as a control group. In the in vivo MRI experiment, the contrast-to-noise ratio (CNR) was higher and persisted longer than that with Gd-DO3A-BT; specifically, the CNR difference was almost five times at 2 h after injection. Gd-DO3A-fen had a binding affinity (Ka) of 6.68 × 106 M−1 for the COX-2 enzyme, which was 2.1-fold higher than that of fenbufen, the original NSAID. In vivo evaluation of anti-inflammatory activity was performed in two animal models. In the turpentine oil-induced model, the mRNA expression levels of inflammatory parameters such as COX-2, TNF-α, IL-1β, and IL-6 were reduced, and in the carrageenan-induced edema model, swelling was suppressed by 72% and there was a 2.88-fold inhibition compared with the saline group. Correlation analysis between in vitro, in silico, and in vivo studies revealed that Gd-DO3A-fen acts as an anti-inflammatory theragnostic agent by directly binding to COX-2.
Differential Impact of Intermittent vs. Sustained Hypoxia on HIF-1, VEGF and Proliferation of HepG2 Cells
Authors: Minoves Mélanie , Hazane-Puch Florence , Moriondo Giorgia , Boutin-Paradis Antoine , Lemarié Emeline , Pépin Jean-Louis , Godin-Ribuot Diane , Briançon-Marjollet Anne
Published: April 2023

Obstructive sleep apnea (OSA) is an emerging risk factor for cancer occurrence and progression, mainly mediated by intermittent hypoxia (IH). Systemic IH, a main landmark of OSA, and local sustained hypoxia (SH), a classical feature at the core of tumors, may act separately or synergistically on tumor cells. Our aim was to compare the respective consequences of intermittent and sustained hypoxia on HIF-1, endothelin-1 and VEGF expression and on cell proliferation and migration in HepG2 liver tumor cells. Wound healing, spheroid expansion, proliferation and migration were evaluated in HepG2 cells following IH or SH exposure. The HIF-1α, endothelin-1 and VEGF protein levels and/or mRNA expression were assessed, as were the effects of HIF-1 (acriflavine), endothelin-1 (macitentan) and VEGF (pazopanib) inhibition. Both SH and IH stimulated wound healing, spheroid expansion and proliferation of HepG2 cells. HIF-1 and VEGF, but not endothelin-1, expression increased with IH exposure but not with SH exposure. Acriflavine prevented the effects of both IH and SH, and pazopanib blocked those of IH but not those of SH. Macitentan had no impact. Thus, IH and SH stimulate hepatic cancer cell proliferation via distinct signaling pathways that may act synergistically in OSA patients with cancer, leading to enhanced tumor progression.
Interplay between Inter-Subunit Rotation of the Ribosome and Binding of Translational GTPases
Authors: Das Ananya , Adiletta Nichole , Ermolenko Dmitri N.
Published: April 2023

Translational G proteins, whose release from the ribosome is triggered by GTP hydrolysis, regulate protein synthesis. Concomitantly with binding and dissociation of protein factors, translation is accompanied by forward and reverse rotation between ribosomal subunits. Using single-molecule measurements, we explore the ways in which the binding of translational GTPases affects inter-subunit rotation of the ribosome. We demonstrate that the highly conserved translation factor LepA, whose function remains debated, shifts the equilibrium toward the non-rotated conformation of the ribosome. By contrast, the catalyst of ribosome translocation, elongation factor G (EF-G), favors the rotated conformation of the ribosome. Nevertheless, the presence of P-site peptidyl-tRNA and antibiotics, which stabilize the non-rotated conformation of the ribosome, only moderately reduces EF-G binding. These results support the model suggesting that EF-G interacts with both the non-rotated and rotated conformations of the ribosome during mRNA translocation. Our results provide new insights into the molecular mechanisms of LepA and EF-G action and underscore the role of ribosome structural dynamics in translation.