bluuebunny/crossref_metadata_2025 huggingface dataset:
Disentangling the effects of temperature and reactive minerals on soil carbon stocks across a thermal gradient in a temperate native forest ecosystem
Authors: Siregar Idri Hastuty , Camps-Arbestain Marta , Kereszturi Gabor , Palmer Alan , Kirschbaum Miko U. F. , Wang Tao
Published: March 2024

Effects of global warming on soil organic carbon (C) can be investigated by comparing sites experiencing different temperatures. However, observations can be affected by covariance of temperature with other environmental properties. Here, we studied a thermal gradient in forest soils derived from volcanic materials on Mount Taranaki (New Zealand) to disentangle the effects of temperature and reactive minerals on soil organic C quantity and composition. We collected soils at four depths and four elevations with mean annual temperatures ranging from 7.3 to 10.5 °C. Soil C stocks were not significantly different across sites (average 162 MgC ha−1 to 85 cm depth, P > .05). Neither aluminium (Al)-complexed C, nor mineral-associated C changed significantly (P > .05) with temperature. The molecular characterisation of soil organic matter showed that plant-derived C declined with increasing temperature, while microbial-processed C increased. Accompanying these changes, soil short-range order (SRO) constituents (including allophane) generally increased with temperature. Results from structural equation modelling revealed that, although a warmer temperature tended to accelerate soil organic C decomposition as inferred from molecular fingerprints, it also exerted a positive effect on soil total C presumably by enhancing plant C input. Despite a close linkage between mineral-associated C and soil organic C, the increased abundance of reactive minerals at 30–85 cm depth with temperature did not increase soil organic C concentration at that depth. We therefore propose that fresh C inputs, rather than reactive minerals, mediate soil C responses to temperature across the thermal gradient of volcanic soils under humid-temperate climatic conditions.
The Potential of Targeting Autophagy-Related Non-coding RNAs in the Treatment of Alzheimer’s and Parkinson’s Diseases
Authors: Talebi Taheri Abdolkarim , Golshadi Zakieh , Zare Hamidreza , Alinaghipour Azam , Faghihi Zahra , Dadgostar Ehsan , Tamtaji Zeinab , Aschner Michael , Mirzaei Hamed , Tamtaji Omid Reza , Nabavizadeh Fatemeh
Published: March 2024

Clearance of accumulated protein aggregates is one of the functions of autophagy. Recently, a clearer understanding of non-coding RNAs (ncRNAs) functions documented that ncRNAs have important roles in several biological processes associated with the development and progression of neurodegenerative disorders. Subtypes of ncRNA, including microRNA (miRNA), long noncoding RNA (lncRNA), and circular RNA (circRNA), are commonly dysregulated in neurodegenerative disorders such as Alzheimer and Parkinson diseases. Dysregulation of these non-coding RNAs has been associated with inhibition or stimulation of autophagy. Decreased miR-124 led to decreased/increased autophagy in experimental model of Alzheimer and Parkinson diseases. Increased BACE1-AS showed enhanced autophagy in Alzheimer disease by targeting miR-214-3p, Beclin-1, LC3-I/LC3-II, p62, and ATG5. A significant increase in NEAT1led to stimulated autophagy in experimental model of PD by targeting PINK1, LC3-I, LC3-II, p62 and miR-374c-5p. In addition, increased BDNF-AS and SNHG1 decreased autophagy in MPTP-induced PD by targeting miR-125b-5p and miR-221/222, respectively. The upregulation of circNF1-419 and circSAMD4A resulted in an increased autophagy by regulating Dynamin-1 and miR-29c 3p, respectively. A detailed discussion of miRNAs, circRNAs, and lncRNAs in relation to their autophagy-related signaling pathways is presented in this study.

Graphical Abstract

Autophagy-related non-coding RNAs in neurodegenerative diseases.
Educational Attainment, Household Income and Wealth Accumulation in Vietnam
Authors: Pham Toan Tan , Lang Ngoc Duc , Ho Chi Minh , Vo Duc Hong
Published: March 2024

This study examines the effects of educational attainment and household income on wealth accumulation in Vietnam using various Vietnam Household Living Standards Surveys (VHLSSs) in 2008, 2018 and 2020. We use the threshold regression to separate households into different groups depending on their assets. We find that owning a university degree is optimal to maximize wealth accumulation for households in 2008. However, post-graduate qualifications are needed for wealth accumulation in 2018 and 2020. For those household heads without a university degree, owning advanced training certificates provides the best opportunity to accumulate household wealth in all three surveys. Besides, our empirical results confirm that household income played no role in wealth accommodation in 2008. Interestingly, household income positively contributes to wealth accumulation for households with assets above the threshold of VND 634.40 m in 2020. However, the effect is negative for households with assets below the threshold of VND 440.25 m in 2018. We also find that households with widowed or divorced household heads are associated with lower wealth accumulation. Another interesting finding is that living in urban areas reduces wealth accumulation for households whose assets are below VND 440.25 m in 2018. These findings largely remain unchanged when Lewbel’s (Lewbel, Journal of Business and Economic Statistics 30:67–80, 2012) instrument variable estimator is used to ensure the robustness of the empirical results.
Modeling the Evolution of the Dislocation Density and Yield Stress of Al over a Wide Range of Temperatures and Strain Rates
Authors: Sadeghi Abbas , Kozeschnik Ernst
Published: March 2024

This paper introduces an extended model for the evolution of internal and wall dislocation densities in pure aluminum during plastic deformation. The approach takes the three internal state variables (3IVM) model as a starting point and advances it by taking into account the dynamic annihilation of immobile/locked dislocations as well as dislocations stored in the subgrain/cell walls. The strength of the material, as one of the properties affected by dislocation density, is used to validate the model. Experimental flow curves for pure Al are taken as the basis for calibration. Compression tests are performed at temperatures from − 196 °C to 500 °C with strain rates of 1, 0.1, and 0.01 s−1. The effect of temperature and strain rate on each state parameter is illustrated and discussed.
Clinical and atopic features of patients with primary eosinophilic colitis: an Italian multicentre study
Authors: Rossi Carlo Maria , Lenti Marco Vincenzo , Merli Stefania , Lo Bello Antonio , Mauro Aurelio , Anderloni Andrea , Ribaldone Davide Giuseppe , Marabotto Elisa , Vernero Marta , Sheijani Shirin Djahandideh , Maniero Daria , Vanoli Alessandro , Klersy Catherine , Savarino Edoardo Vincenzo , Di Sabatino Antonio
Published: March 2024

Eosinophilic colitis (EC) is the rarest among primary eosinophilic gastrointestinal disorders (EGID). EC is underdiagnosed due to its blurred and proteiform clinical manifestations. To explore the clinical and atopic characteristic of EC adult patients, the diagnostic delay, and relapse-associated factors, by comparison with patients with eosinophilic esophagitis (EoE) and irritable bowel syndrome (IBS). EC patients followed-up at four clinics were included, and clinical, histopathological, and laboratory data were retrieved. As control groups, age-matched patients with EoE and IBS were recruited. Allergy tests included skin prick test and serum specific IgE. Diagnostic delay was assessed. Overall, data from 73 patients were retrieved, including 40 with EC (median age 39 years IQR 22.5–59, F:M 2.1:1), 12 with EoE (F:M ratio: 1:5), and 21 with IBS (F:M ratio: 1:0.9). The most common features in EC patients were female sex (67.5%), atopy (77.5%), abdominal pain/distention (70%), diarrhoea (77.5%), and faecal calprotectin elevation (22.5%). Blood eosinophils were elevated in EoE, but not in EC (p < 0.001), while ECP did not differ across the three groups (p = 0.4). The frequency of allergen sensitization reached 25% of patients. Several frequent pan-allergens for this region were present. The overall diagnostic delay was 10 months (IQR 4–15). Factors contributing to a greater diagnostic delay were atopy, weight loss, and a previous misdiagnosis. EC is mostly a diagnosis of exclusion, burdened by a substantial diagnostic delay. In female patients the presence of allergen sensitization, abdominal symptoms and faecal calprotectin elevation should raise the suspicion of EC.
‘The highest confidence that repetitive head collisions causes chronic traumatic encephalopathy’? Analysing the scientific knowledge in the Rugby Union concussion litigation of England and Wales
Authors: Kilgallon Jonathan
Published: March 2024

295 rugby players have begun legal proceedings against World Rugby, the Rugby Football Union and the Welsh Rugby Union. The claimants report they are suffering from chronic traumatic encephalopathy. Among the allegations against the defendants is that the player safety regulations (i.e. the Pitch Side Concussion Assessment and Return to Play protocols) in place at the time of injury were done without proper medical foresight and increased the risks of concussion to players. World Rugby’s safety regulations are informed by the work of the Concussion in Sport Group which maintains that the link between collisions and concussion is unclear. However, recent studies report an unprecedented confidence that sports-related concussion causes brain disease. In 2022, the Concussion in Sport Groups lead author resigned due to 10 counts of plagiarism. This saga risks damaging public respect for both the safety regulations and the science underpinning it. This article examines the state of the scientific literature and considers the challenges in proving (1) the governing bodies breached their duty of care and; (2) the causal link between collisions and brain injury. This article argues that 2022 marked the year that defendants can no longer deny the dangers of sports-related concussion, however, defining the moment when the risks ought to be known remains onerous. On causation, this article examines the principles of material contribution, doubling of the risk, and the material increase in risk. The article concludes with policy considerations inviting an extension of the material increase in risk principle to sports settings.
Perioperative outcome, long-term mortality and time trends in elderly patients undergoing low-, intermediate- or major non-cardiac surgery
Authors: Tjeertes E. K. M. , Simoncelli T. F. W. , van den Enden A. J. M. , Mattace-Raso F. U. S. , Stolker R. J. , Hoeks S. E.
Published: March 2024

Background

Decision-making whether older patients benefit from surgery can be a difficult task. This report investigates characteristics and outcomes of a large cohort of inpatients, aged 80 years and over, undergoing non-cardiac surgery.

Methods

This observational study was performed at a tertiary university medical centre in the Netherlands. Patients of 80 years or older undergoing elective or urgent surgery from January 2004 to June 2017 were included. Outcomes were length of stay, discharge destination, 30-day and long-term mortality. Patients were divided into low-, intermediate and high-risk surgery subgroups. Univariable and multivariable logistic regression were used to evaluate the association of risk factors and outcomes. Secondary outcomes were time trends, assessed with Mantel–Haenszel chi-square test.

Results

Data of 8251 patients, undergoing 19,027 surgical interventions were collected from the patients’ medical record. 7032 primary procedures were suitable for analyses. Median LOS was 3 days in the low-risk group, compared to six in the intermediate- and ten in the high-risk group. Median LOS of the total cohort decreased from 5.8 days (IQR 1.9–14.5) in 2004–2007 to 4.6 days (IQR 1.9–9.0) in 2016–2017. Three quarters of patients were discharged to their home. Postoperative 30-day mortality in the low-risk group was 2.3%. In the overall population 30-day mortality was high and constant during the study period (6.7%, ranging from 4.2 to 8.4%).

Conclusion

Patients should not be withheld surgery solely based on their age. However, even for low-risk surgery, the mortality rate of more than 2% is substantial. Deciding whether older patients benefit from surgery should be based on the understanding of individual risks, patients’ wishes and a patient-centred plan.
Time delay recurrent neural networks via PID control law to complex dynamic systems
Authors: Perez Jose P. , Perez P Joel , Perez D J. Javier , Estevane Pablo Salinas
Published: March 2024

This paper presents an extension to the theory on the analysis of stability of complex systems with time delay of fractional order; the previous study is based on the theory of functions of Lyapunov–Krasovskii and the fractional-order PID control law. The analytical results are illustrated by the simulation of fractional nonlinear systems interconnected with time delay which are forced to follow complex trajectories as chaotic systems.
Once upon a time: the glucagon stimulation test in diagnosing adult GH deficiency
Authors: Cuboni D. , Caputo M. , Ghigo E. , Aimaretti G. , Gasco V.
Published: March 2024

Purpose

The clinical features of adult GH deficiency (GHD) are nonspecific, and its diagnosis is established through GH stimulation testing, which is often complex, expensive, time-consuming and may be associated with adverse side effects. Moreover, diagnosing adult GHD can be challenging due to the influence of age, gender, and body mass index on GH peak at each test. The insulin tolerance test (ITT), GHRH + arginine test, glucagon stimulation test (GST), and, more recently, testing with macimorelin are all recognized as useful in diagnosing adult GHD. To date GST is still little used, but due to the unavailability of the GHRH all over the world and the high cost of macimorelin, in the next future it will probably become the most widely used test when ITT is contraindicated. The aim of the present review is to describe the current knowledge on GST.

Methods

Narrative review.

Results

In the last years several studies have suggested some changes in the original GST protocol and have questioned its diagnostic accuracy when the classic GH cut-point of 3 μg/L is used, suggesting to use a lower GH cut-point to improve its sensitivity and specificity in overweight/obese patients and in those with lower pretest GHD probability.

Conclusion

This document provides an update on the utility of GST, summarizes how to perform the test, shows which cut-points should be used in interpreting the results, and discusses its drawbacks and caveats referring to the most recent studies.
First report of Cryptostroma corticale on Aesculus hippocastanum causing sooty bark disease in Germany
Authors: Brenken Ann-Christin , Kehr Rolf , Riebesehl Janett , Esch Jonas , Enderle Rasmus
Published: March 2024

Cryptostroma corticale is the causal agent of sooty bark disease, which was first described in the middle of the last century and has developed in recent years to a relevant threat for Acer spp. trees in Central Europe. Triggered by extreme heat and drought, this tree disease is becoming more and more important in the course of climate change. Acer pseudoplatanus is a particularly affected tree species, but the disease has also been observed on other Acer spp., and there is some indication that there are suitable hosts outside the Acer genus. In literature, Aesculus hippocastanum was mentioned twice to be a host, however, without any proof or details. With this study, we verify the assumption that A. hippocastanum is a host of C. corticale by morphological and phylogenetic analyses based on a case in Germany. Furthermore, we provide microscope pictures of microtome sections of the specimens, showing the spore production of C. corticale on A. hippocastanum.
Activation of carbon dioxide on zinc oxide surfaces doped with cerium
Authors: Doria Angeles , Ponnefz Durango Rafael E. , Alcalá Varilla Luis A.
Published: March 2024

The adsorption of carbon dioxide on the surfaces of zinc oxide doped with cerium was studied. For this, a theoretical study was carried out using computational simulations based on density functional theory to determine possible improvements in photocatalytic activity. for the capture and dissociation of carbon dioxide. Calculations were performed using density functional theory within the Perdew-Burke-Ernzerhof generalized gradient approximation, also the Hubbard correction together with ultrasmooth atomic pseudopotentials and a basic plane wave implemented in the Quantum-ESPRESSO package. The doping concentration level considered in this work was 6.25%, among the results, the semiconducting character of zinc oxide was evident from the density of states calculations, it was also found that by adding cerium impurities to zinc oxide, the values of the lengths and angles of the bonds vary a little, this may be due to the small difference in covalent radius that the zinc atom has with respect to the cerium atom, consequently, changes occurred in the electronic properties that consist in intermediate states in the energy bandgap located around the Fermi energy. This may suggest that the cerium-doped zinc oxide system can probably absorb visible light, which could lead to possible improvements in the photocatalytic properties of the material. Furthermore, we found that by adding carbon dioxide to the cerium-doped zinc oxide surface, the carbon dioxide is activated and this could suggest the dissociation or reduction of this contaminant.
Identification of Key Factors in Cartilage Tissue During the Progression of Osteoarthritis Using a Non-targeted Metabolomics Strategy
Authors: Sun Shiyu , Chen Minghui , Zhang Tingting , Wang Yanyan , Shen Weijun , Zhang Tao , Liu Jian , Lan Haidan , Zhao Jianyuan , Lin Fuqing , Zhao Xuan
Published: March 2024

This research was to reveal the key factors in the progression of osteoarthritis (OA) using non-targeted metabolomics and to find targeted therapies for patients with OA. Twenty-two patients with knee OA scheduled for total knee arthroplasty were divided into two groups: Kellgren–Lawrence (KL) grade 3 (n = 16) and grade 4 (n = 6), according to plain X-rays of the knee. After the operation, the cartilages of femur samples were analyzed using non-targeted metabolomics. When compared with grade 3 patients, the levels of choline, 2-propylpiperidine, rhamnose, and monomethyl glutaric acid were higher; while 1-methylhistamine, sphingomyelin (SM) (d18:1/14:0), zeranol, 3- (4-hydroxyphenyl)-1-propanol, 5-aminopentanamide, dihydrouracil, 2-hydroxypyridine, and 3-amino-2-piperidone were lower in grade 4 patients. Furthermore, some metabolic pathways were found to be significantly different in two groups such as the pantothenate and coenzyme A (CoA) biosynthesis pathway, the glycerophospholipid metabolism pathway, histidine metabolism pathway, lysine degradation pathway, glycine, serine and threonine metabolism pathway, fructose and mannose metabolism pathway, the pyrimidine metabolism pathway, and beta-alanine metabolism pathway. This work used non-targeted metabolomics and screened out differential metabolites and metabolic pathways, providing a reliable theoretical basis for further study of specific markers and their specific pathways in the progression of OA.
Equivariant Hodge polynomials of heavy/light moduli spaces
Authors: Kannan Siddarth , Serpente Stefano , Yun Claudia He
Published: March 2024

Let

$\overline {\mathcal {M}}_{g, m|n}$

denote Hassett’s moduli space of weighted pointed stable curves of genus g for the heavy/light weight data

$$\begin{align*}\left(1^{(m)}, 1/n^{(n)}\right),\end{align*}$$

and let

$\mathcal {M}_{g, m|n} \subset \overline {\mathcal {M}}_{g, m|n}$

be the locus parameterizing smooth, not necessarily distinctly marked curves. We give a change-of-variables formula which computes the generating function for

$(S_m\times S_n)$

-equivariant Hodge–Deligne polynomials of these spaces in terms of the generating functions for

$S_{n}$

-equivariant Hodge–Deligne polynomials of

$\overline {\mathcal {M}}_{g,n}$

and

$\mathcal {M}_{g,n}$

.
Mobility, Migration, and Mobile Migration
Authors: Milioni Anna
Published: March 2024

Our world is mobile. People move, either within the state or from one state to another, to access opportunities, to improve their living conditions, or to start afresh. Yet, we usually assume that migration is an exceptional activity that leads to permanent settlement. In this paper, I invite us to reconsider this assumption. First, I analyse several ways in which people experience mobility in contemporary societies. Then, I turn to migration, as a specific form of mobility. I distinguish between a legal/administrative, a social, and a self-identification-based approach to migration, demonstrating that they lead to divergent definitions of who is a migrant. I proceed by introducing the concept of mobile migration and by developing a typology of mobile migrants. I conclude that, in order to devise migration policies that treat migrants fairly, a careful consideration of the claims and interests of the different types of mobile migrants is necessary.
Exercise therapy for chronic symptomatic peripheral artery	disease
Authors: Mazzolai Lucia , Belch Jill , Venermo Maarit , Aboyans Victor , Brodmann Marianne , Bura-Rivière Alessandra , Debus Sebastien , Espinola-Klein Christine , Harwood Amy E. , Hawley John A. , Lanzi Stefano , Madarič Juraj , Mahé Guillaume , Malatesta Davide , Schlager Oliver , Schmidt-Trucksäss Arno , Seenan Chris , Sillesen Henrik , Tew Garry A. , Visonà Adriana
Published: March 2024

Summary: All guidelines worldwide strongly recommend exercise as a pillar in the management of patients affected by lower extremity peripheral artery disease (PAD). Exercise therapy in this setting presents different modalities, and a structured programme provides optimal results. This clinical consensus paper is intended to promote and assist the set up of comprehensive exercise programmes and best advice for patients with symptomatic chronic PAD. Different exercise training protocols specific for patients with PAD are presented. Data on patient assessment and outcome measures are described based on the current best evidence. The document ends by highlighting supervised exercise programme access disparities across Europe and the evidence gaps requiring further research.
EMM EMUS Observations of Hot Oxygen Corona at Mars: Radial Distribution and Temporal Variability
Authors: Chirakkil Krishnaprasad , Deighan Justin , Chaffin Michael S. , Jain Sonal K. , Lillis Robert J. , Raghuram Susarla , Holsclaw Greg , Brain David A. , Thiemann Ed , Chamberlin Phil , Fillingim Matthew O. , Evans J. Scott , England Scott , AlMatroushi Hessa , AlMazmi Hoor , Eparvier Frank , Gacesa Marko , El‐Kork Nayla , Curry Shannon M.
Published: March 2024

We present the first observations of the dayside coronal oxygen emission in far ultraviolet (FUV) measured by the Emirates Mars Ultraviolet Spectrometer (EMUS) onboard the Emirates Mars Mission (EMM). The high sensitivity of EMUS is providing an opportunity to observe the tenuous oxygen corona in FUV, which is otherwise difficult to observe. Oxygen resonance fluorescence emission at 130.4 nm provides a measurement of the upper atmospheric and exospheric oxygen. More than 500 oxygen corona profiles are constructed using the long–exposure time cross–exospheric mode (OS4) of EMUS observations. These profiles range from ∼200 km altitude up to several Mars radii (>6 RM) across all seasons and for two Mars years. Our analysis shows that OI 130.4 nm is highly correlated with solar irradiance (solar photoionizing and 130.4 nm illuminating irradiances) as well as changes in the Sun–Mars distance. The prominent short term periodicity in oxygen corona brightness is consistent with the solar rotation period (quasi–27–day). A comparison between the perihelion seasons of Mars Year (MY) 36 and MY 37 shows interannual variability with enhanced emission intensities during MY 37, due to the rise of Solar Cycle 25. These observations show a highly variable oxygen corona, which has significant implications on constraining the photochemical escape of atomic oxygen from Mars.
Recent Widespread Deposition in the Martian North and South Polar Layered Deposits as Revealed by Multiband SHARAD Surface Reflectivity
Authors: Jawin Erica R. , Campbell Bruce A.
Published: March 2024

The north and south polar layered deposits (PLD) on Mars are composed of stacks of layered ice and dust, but the SPLD is approximately twice as bright as the NPLD in 20‐MHz radar echoes. We use Shallow Radar (SHARAD) data in ∼4‐MHz bands centered on 17.5 MHz (“L”) and 22.5 MHz (“H”) to determine whether radar reflectivity variations are due to scattering effects related to closely spaced, near‐surface dielectric layering. We mapped the ratio of the surface echo power at the two frequencies (H/L) for both PLDs. The NPLD has large areas where H and L echo power differ, consistent with destructive interference in the H band within the uppermost ∼20 m. The SPLD is dominated by H ∼ L (unity), except for isolated regions in and near the residual CO2 cap and Australe Lingula. The H/L variations can be partly explained by near‐surface structure, where large variations in H/L match locations with numerous near‐surface reflecting interfaces, and locations where H ∼ L may contain few such reflectors. There is no obvious connection between H/L and surface morphology, but the distribution of non‐unity H/L resembles the extent of a widespread, recent accumulated package (WRAP) at both poles. The spatial association between H/L and WRAP and interference indicated by H/L suggests that large regions in the NPLD—and isolated areas in the SPLD—are characterized by shallow layer(s) of consistent thickness/separation potentially deposited within the past few tens of kyr as Mars emerged from the last obliquity‐driven ice age.
Water‐Ice Dominated Spectra of Saturn's Rings and Small Moons From JWST
Authors: Hedman M. M. , Tiscareno M. S. , Showalter M. R. , Fletcher L. N. , King O. R. T. , Harkett J. , Roman M. T. , Rowe‐Gurney N. , Hammel H. B. , Milam S. N. , El Moutamid M. , Cartwright R. J. , de Pater I. , Molter E. M.
Published: March 2024

JWST measured the infrared spectra of Saturn's rings and several of its small moons (Epimetheus, Pandora, Telesto, and Pallene) as part of Guaranteed Time Observation program 1247. The NIRSpec instrument obtained near‐infrared spectra of the small moons between 0.6 and 5.3 microns, which are all dominated by water‐ice absorption bands. The shapes of the water‐ice bands for these moons suggests that their surfaces contain variable mixes of crystalline and amorphous ice or variable amounts of contaminants and/or sub‐micron ice grains. The near‐infrared spectrum of Saturn's A ring has exceptionally high signal‐to‐noise between 2.7 and 5 microns and is dominated by features due to highly crystalline water ice. The ring spectrum also confirms that the rings possess a 2%–3% deep absorption at 4.13 microns due to deuterated water ice previously seen by the Visual and Infrared Mapping Spectrometer onboard the Cassini spacecraft. This spectrum also constrains the fundamental absorption bands of carbon dioxide and carbon monoxide and may contain evidence for a weak aliphatic hydrocarbon band. Meanwhile, the MIRI instrument obtained mid‐infrared spectra of the rings between 4.9 and 27.9 microns, where the observed signal is a combination of reflected sunlight and thermal emission. This region shows a strong reflectance peak centered around 9.3 microns that can be attributed to crystalline water ice. Since both the near and mid‐infrared spectra are dominated by highly crystalline water ice, they should provide a useful baseline for interpreting the spectra of other objects in the outer solar system with more complex compositions.
Identifying key antioxidative stress factors regulating Nrf2 in the genioglossus with human umbilical cord mesenchymal stem-cell therapy
Authors: Guo Haixian , Liu Yue , Yu Xinlu , Tian Na , Liu Yan , Yu Dan
Published: March 2024

Intermittent hypoxia in patients with obstructive sleep apnea (OSA) hypopnea syndrome (OSAHS) is associated with pharyngeal cavity collapse during sleep. The effect of human umbilical cord mesenchymal stem cells (HUCMSCs) on OSA-induced oxidative damage in the genioglossus and whether nuclear factor erythroid 2-related factor 2 (Nrf2) or its upstream genes play a key role in this process remains unclear. This study aimed to identify the key factors responsible for oxidative damage during OSAHS through Nrf2 analysis and hypothesize the mechanism of HUCMSC therapy. We simulated OSA using an intermittent hypoxia model, observed the oxidative damage in the genioglossus and changes in Nrf2 expression during intermittent hypoxia, and administered HUCMSCs therapy. Nrf2 initially increased, then decreased, aggravating the oxidative damage in the genioglossus; Nrf2 protein content decreased during hypoxia. Using transcriptomics, we identified seven possible factors in HUCMSCs involved in ameliorating oxidative stress by Nrf2, of which DJ-1 and MEF2A, showing trends similar to Nrf2, were selected by polymerase chain reaction. HUCMSCs may reduce oxidative stress induced by intermittent hypoxia through Nrf2, and the possible upstream target genes in this process are MEF2A and DJ-1. Further studies are needed to verify these findings.
Morphological and morphometric anatomy of the lesser occipital nerve and its possible clinical relevance
Authors: Saglam Latif , Coskun Osman , Gayretli Ozcan
Published: March 2024

The lesser occipital nerve (LON) has one of the most variations among occipital nerves. We aimed to investigate morphological and morphometric features of LON. A total of 24 cadavers, 14 males (58%) and 10 females (42%), were dissected bilaterally. LON was classified into 3 types. The number of branches and the perpendicular distances of the point where LON emerged from the posterior border of sternocleidomastoid muscle to vertical and transverse lines passing through external occipital protuberance were determined. The shortest distance between LON and great auricular nerve (GAN), and linear distance of LON to its branching point were measured. The most common variant was Type 1 (30 sides, 62.5%), followed by Type 2 (12 sides, 25%) and Type 3 (6 sides, 12.5%), respectively. In males, Type 1 (22 sides, 78.6%) was the most common, while Type 1 (8 sides, 40%) and Type 2 (8 sides, 40%) were equally common and the most common in females. On 48 sides, 2–9 branches of LON were observed. The perpendicular distance of said point to vertical and transverse lines was meanly 63.69 ± 11.28 mm and 78.83 ± 17.21 mm, respectively. The shortest distance between LON and GAN was meanly 16.62 ± 10.59 mm. The linear distance of LON to its branching point was meanly 31.24 ± 15.95 mm. The findings reported in this paper may help clinicians in estimating the location of the nerve and/or its branches for block or decompression surgery as well as preservation of LON during related procedures.
Serum ACE2 and S19P gene polymorphism in Egyptian patients with COVID-19 infection: correlation with disease severity
Authors: Gaber Dalia A. , Shokr Mohamed , Shaker Olfat , Zaki Kamelia Ahmed , Khalil Haidy Samir , Wahb Amany M.
Published: March 2024

The expression of ACE2 is linked to disease severity in COVID-19 patients. The ACE2 receptor gene polymorphisms are considered determinants for SARS-CoV-2 infection and its outcome. In our study, serum ACE2 and its genetic variant S19P rs73635825 polymorphism were investigated in 114 SARS-CoV-2 patients. The results were compared with 120 control subjects. ELISA technique and allele discrimination assay were used for measuring serum ACE2 and genotype analysis of ACE2 rs73635825. Our results revealed that serum ACE2 was significantly lower in SARS-CoV-2 patients (p = 0.0001), particularly in cases with hypertension or diabetes mellitus. There was a significant difference in the genotype distributions of ACE2 rs73635825 A > G between COVID-19 patients and controls (p-value = 0.001). A higher frequency of the heterozygous AG genotype (65.8%) was reported in COVID-19 patients. The G allele was significantly more common in COVID-19 patients (p < 0.0001). The AG and GG genotypes were associated with COVID-19 severity as they were correlated with abnormal laboratory findings, GGO, CXR, and total severity scores with p < 0.05. Our results revealed that the ACE2 S19P gene variant is correlated with the incidence of infection and its severity, suggesting the usefulness of this work in identifying the susceptible population groups for better disease control.
Unlocking stray light mysteries in the CoRot baffle with the time-of-flight method
Authors: Clermont L. , Blain P. , Khaddour W. , Uhring W.
Published: March 2024

Stray light (SL) has emerged as a primary limiting factor for space telescopes. Pre-launch testing is essential for validating performance and identifying potential issues. However, traditional methods do not enable the decomposition and identification of individual SL contributors. Consequently, when problems arise, resolving them often involves a cumbersome and risky trial-and-error approach. The time-of-flight (ToF) method was recently introduced, employing a pulsed laser source and ultrafast sensor to characterize individual SL contributors. A proof of concept was achieved using a simple three-lens system. In this paper, we apply the ToF method to a real space optical system: the spare model of the CoRoT baffle. We successfully measured individual SL contributors over a dynamic range of 10−11, identifying direct scattering on vane edges and two-step scattering paths. Our results provide a performance breakdown, differentiating intrinsic baffle SL from contributions arising from experimental conditions. Notably, the ToF method allowed us to discriminate air scattering, eliminating the need for expensive vacuum testing. The ToF provides unparallel insights, including defects identification. For instance, we identified the presence of localized dust particles causing significant SL. These results confirm the utility of the ToF method even for the most challenging space systems.
Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity
Authors: Taha Noha Madbouly , Youssef Fady Sayed , Auda Hend M. , El-Bahy Mohamed M. , Ramadan Reem M.
Published: March 2024

Trichinellosis is a worldwide zoonotic disease. The majority of currently available anti-trichinellosis medications exhibit inadequate efficacy. The efficacy of a natively prepared new formulation of silver nanoparticles (Ag-NPs) was evaluated in the treatment of Trichinella spiralis (T. spiralis) infection in mice alone and combined with multivitamin-mineral (MM). After investigating the product’s biological and pharmacological characteristics, its therapeutic dose was estimated to be Ag-NPs at 21.5 mg/kg B.W. This dose was orally inoculated to experimentally infected mice at 3–5 days post-inoculation (dpi) against the mature worms, at 8–10 dpi against the newborn larvae, and at 33–35th dpi against the encapsulated larvae. Each treatment’s efficacy was assessed by scarifying control and treated mice 3 days post-treatment. The drug alone or in supplement form has a high trichinocidal effect exceeding that of the reference drug. Early treatment (3–5 dpi) by Ag-NPs or Ag-NPs + MM and albendazole revealed high efficacy against the intestinal stage, reaching 93.3%, 94.7%, and 90.6% for the three treatments, respectively. The materials causing a significant (P-value < 0.001) decrease in the mean encapsulated larvae reached 86.61%, 89.07%, and 88.84%/gm of muscles using the three treatments, respectively. Moreover, all larvae extracted from Ag-NPs-treated groups failed to induce infection post-inoculation in new mice. Additionally, combining the material with MM proved to overcome the reversible adverse effects of silver material on the estimated redox parameters and liver and kidney biomarkers, denoting its ability to alleviate Ag-NP toxicity. In conclusion, the high trichinocidal effect of Ag-NPs against the adult and encapsulated larvae during a short inoculation period introduced Ag-NPs as an alternative to other nematicidal drugs.
Integrating core physics and machine learning for improved parameter prediction in boiling water reactor operations
Authors: Oktavian M. R. , Nistor J. , Gruenwald J. T. , Xu Y.
Published: March 2024

This study introduces a novel method for enhancing Boiling Water Reactor (BWR) operation simulations by integrating machine learning (ML) models with conventional simulation techniques. The ML model is trained to identify and correct errors in low-fidelity simulation outputs, traditionally derived from core physics computations. These corrections aim to align the low-fidelity results closely with high-fidelity data. Precise predictions of nuclear reactor parameters like core eigenvalue and power distribution are crucial for efficient fuel management and adherence to technical specifications. Current high-fidelity transport calculations, while accurate, are impractical for real-time predictions due to extensive computational demands. Our approach, therefore, utilizes the standard two-step simulation process-assembly-level lattice physics calculations followed by whole-core nodal diffusion computations-to generate initial results, which are then refined using the ML-based error correction model. The methodology focuses on improving simulation accuracy in regular BWR operations rather than developing a universal ML predictor for reactor physics. By training an advanced neural network model on the difference in high-fidelity and low-fidelity simulations, the model can reduce the nodal power error from low-fidelity simulations to around 1% on average and the core eigenvalue down to under 100 pcm. This result is under the condition of the normal variations of control rod pattern and core flow rate changes in standard BWR operations used in the training and evaluation of the machine learning model. This work suggests a promising approach for achieving more accurate, computationally feasible simulation solutions in nuclear reactor operation and management.
Precision cancer classification using liquid biopsy and advanced machine learning techniques
Authors: Eledkawy Amr , Hamza Taher , El-Metwally Sara
Published: March 2024

Cancer presents a significant global health burden, resulting in millions of annual deaths. Timely detection is critical for improving survival rates, offering a crucial window for timely medical interventions. Liquid biopsy, analyzing genetic variations, and mutations in circulating cell-free, circulating tumor DNA (cfDNA/ctDNA) or molecular biomarkers, has emerged as a tool for early detection. This study focuses on cancer detection using mutations in plasma cfDNA/ctDNA and protein biomarker concentrations. The proposed system initially calculates the correlation coefficient to identify correlated features, while mutual information assesses each feature's relevance to the target variable, eliminating redundant features to improve efficiency. The eXtrem Gradient Boosting (XGBoost) feature importance method iteratively selects the top ten features, resulting in a 60% dataset dimensionality reduction. The Light Gradient Boosting Machine (LGBM) model is employed for classification, optimizing its performance through a random search for hyper-parameters. Final predictions are obtained by ensembling LGBM models from tenfold cross-validation, weighted by their respective balanced accuracy, and averaged to get final predictions. Applying this methodology, the proposed system achieves 99.45% accuracy and 99.95% AUC for detecting the presence of cancer while achieving 93.94% accuracy and 97.81% AUC for cancer-type classification. Our methodology leads to enhanced healthcare outcomes for cancer patients.
Occlusal characteristics in modern humans with tooth agenesis
Authors: Alamoudi Ragda , Kanavakis Georgios , Oeschger Elias S. , Halazonetis Demetrios , Gkantidis Nikolaos
Published: March 2024

Non-syndromic permanent tooth agenesis affects a significant proportion of the population, especially if third molars are considered. Although tooth agenesis has been linked to a smaller craniofacial size, reduced facial convexity and a shorter skeletal face, the occlusal characteristics of individuals with tooth agenesis remain largely unexplored. Therefore, this study investigated potential associations between tooth agenesis and metric occlusal traits in 806 individuals (491 with 4.1 missing teeth per subject, including third molars, and 315 without any tooth agenesis). Dentoskeletal morphology was defined through anatomical landmarks on pre-treatment cephalometric radiographs. Multivariate regression models, adjusted for sex and age, showed that tooth agenesis was significantly associated with a reduced overjet, an increased interincisal angle, and shorter upper and lower dental arch lengths, but not with overbite. Moreover, apart from reduced tooth length and dentoalveolar effects, as the number of missing teeth increased the upper front teeth were progressively retruded according to the craniofacial complex and to the face. Thus, tooth agenesis has a substantial influence on dental and occlusal characteristics, as well as on the sagittal position and inclination of anterior teeth. These findings emphasize the necessity for personalized, multidisciplinary approaches in individuals with multiple agenesis to successfully meet treatment goals.
Study of multi-dimensional problems arising in wave propagation using a hybrid scheme
Authors: Liu Jinxing , Nadeem Muhammad , Osman M. S. , Alsayaad Yahya
Published: March 2024

Many scientific phenomena are linked to wave problems. This paper presents an effective and suitable technique for generating approximation solutions to multi-dimensional problems associated with wave propagation. We adopt a new iterative strategy to reduce the numerical work with minimum time efficiency compared to existing techniques such as the variational iteration method (VIM) and homotopy analysis method (HAM) have some limitations and constraints within the development of recurrence relation. To overcome this drawback, we present a Sawi integral transform ($$\mathbb {S}$$
S
T) for constructing a suitable recurrence relation. This recurrence relation is solved to determine the coefficients of the homotopy perturbation strategy (HPS) that leads to the convergence series of the precise solution. This strategy derives the results in algebraic form that are independent of any discretization. To demonstrate the performance of this scheme, several mathematical frameworks and visual depictions are shown.
MicroRNA-561-3p indirectly regulates the PD-L1 expression by targeting ZEB1, HIF1A, and MYC genes in breast cancer
Authors: Yousefi Atena , Sotoodehnejadnematalahi Fattah , Nafissi Nahid , Zeinali Sirous , Azizi Masoumeh
Published: March 2024

Globally, breast cancer is the second most common cause of cancer-related deaths among women. In breast cancer, microRNAs (miRNAs) are essential for both the initiation and development of tumors. It has been suggested that the tumor suppressor microRNA-561-3p (miR-561-3p) is crucial in arresting the growth of cancer cells. Further research is necessary to fully understand the role and molecular mechanism of miR-561 in human BC. The aim of this study was to investigate the inhibitory effect of miR-561-3p on ZEB1, HIF1A, and MYC expression as oncogenes that have the most impact on PD-L1 overexpression and cellular processes such as proliferation, apoptosis, and cell cycle in breast cancer (BC) cell lines. The expression of ZEB1, HIF1A, and MYC genes and miR-561-3p were measured in BC clinical samples and cell lines via qRT-PCR. The luciferase assay, MTT, Annexin-PI staining, and cell cycle experiments were used to assess the effect of miR-561-3p on candidate gene expression, proliferation, apoptosis, and cell cycle progression. Flow cytometry was used to investigate the effects of miR-561 on PD-L1 suppression in the BC cell line. The luciferase assay showed that miRNA-561-3p targets the 3′-UTRs of ZEB1, HIF1A and MYC genes significantly. In BC tissues, the qRT-PCR results demonstrated that miR-561-3p expression was downregulated and the expression of ZEB1, HIF1A and MYC genes was up-regulated. It was shown that overexpression of miR-561-3p decreased PD-L1 expression and BC cell proliferation, and induced apoptosis and cell cycle arrest through downregulation of candidate oncogenes. Furthermore, inhibition of candidate genes by miR-561-3p reduced PD-L1 at both mRNA and protein levels. Our research investigated the impact of miR-561-3p on the expression of ZEB1, HIF1A and MYC in breast cancer cells for the first time. Our findings may help clarify the role of miR-561-3p in PD-L1 regulation and point to this miR as a potential biomarker and novel therapeutic target for cancer immunotherapy.
Regional pulmonary perfusion, blood volume, and their relationship change in experimental early ARDS
Authors: Santos Arnoldo , Motta-Ribeiro Gabriel C. , de Prost Nicolas , Tucci Mauro R. , Wellman Tyler J. , Vidal Melo Marcos F. , Winkler Tilo
Published: March 2024

Regional pulmonary perfusion (Q) has been investigated using blood volume (Fb) imaging as an easier-to-measure surrogate. However, it is unclear if changing pulmonary conditions could affect their relationship. We hypothesized that vascular changes in early acute respiratory distress syndrome (ARDS) affect Q and Fb differently. Five sheep were anesthetized and received lung protective mechanical ventilation for 20 h while endotoxin was continuously infused. Using dynamic 18F-FDG and 13NN Positron Emission Tomography (PET), regional Fb and Q were analysed in 30 regions of interest (ROIs) and normalized by tissue content (Fbn and Qn, respectively). After 20 h, the lung injury showed characteristics of early ARDS, including gas exchange and lung mechanics. PET images of Fbn and Qn showed substantial differences between baseline and lung injury. Lung injury caused a significant change in the Fbn-Qn relationship compared to baseline (p < 0.001). The best models at baseline and lung injury were Fbn = 0.32 + 0.690Qn and Fbn = 1.684Qn–0.538Qn2, respectively. Endotoxine-associated early ARDS changed the relationship between Fb and Q, shifting from linear to curvilinear. Effects of endotoxin exposure on the vasoactive blood flow regulation were most likely the key factor for this change limiting the quantitative accuracy of Fb imaging as a surrogate for regional Q.