bluuebunny/crossref_metadata_2025 huggingface dataset:
Whole-Transcriptome Analysis of LncRNAs Mediated ceRNA Regulation in Granulosa Cells Isolated From Healthy and Atresia Follicles of Chinese Buffalo
Authors: Pan Yu , Yang Sufang , Cheng Juanru , Lv Qiao , Xing Qinghua , Zhang Ruimen , Liang Jingyuan , Shi Deshun , Deng Yanfei
Published: July 2021

Granulosa cells (GCs) are the main supporting cells in follicles and play an important role in the regulation of oocyte maturation and follicular atresia. Accumulating evidence indicates that non-coding RNAs participate in regulation of the physiological function of GCs. However, whole-transcriptome analysis for GCs of buffalo has yet to be reported. In this study, healthy follicles (HFs) and atretic follicles (AFs) were defined according to the apoptosis rate of GCs and the hormone level in follicular fluid. GCs were collected from HFs and AFs (n = 15, 5 < n < 8 mm) for whole-transcriptome analysis using second-generation high-throughput sequencing. A total of 1,861 and 1,075 mRNAs, 159 and 24 miRNAs, and 123 and 100 lncRNAs, were upregulated and downregulated between HFs and AFs, respectively. Enrichment of functions and signaling pathways of these differentially expressed (DE) genes showed that most of DEmRNAs and targets of DEmiRNAs were annotated to the categories of ECM–receptor interaction and focal adhesion, as well as PI3K-AKT, mTOR, TGF-beta, Rap1, and estrogen signaling pathways. The competing endogenous RNA (CeRNA) network was also constructed based on the ceRNA theory which further revealed regulatory roles of these DERNAs in GCs of buffalo follicles. Finally, we validated that lnc4040 regulated the expression of Hif1a as miR-709 sponge in a ceRNA mechanism, suggesting their critical functions in GCs of buffalo follicles. These results show that lncRNAs are dynamically expressed in GCs of HFs and AFs, and interacting with target genes in a ceRNA manner, suggesting their critical functions in buffalo follicular development and atresia.
Mindfulness y neurobiología
Authors: M. Simón Vicente
Published: July 2021

En la actualidad asistimos a la introducción de las técnicas de mindfulness en la educación, la psicoterapia y en todo el ámbito científico en general. Una de las razones de este auge son los conocimientos proporcionados por las modernas técnicas de investigación neurobiológica. En este trabajo revisamos algunos de los efectos de mindfulness sobre los procesos perceptivos (apertura a la novedad), el estilo afectivo (afecto positivo y aproximación), la inmunidad (incremento) y la empatía (facilitación). Los hallazgos neurobiológicos sugieren que la práctica de mindfulness activa y fortalece diversas regiones cerebrales (especialmente la corteza prefrontal) encargadas de los procesos integradores más específicamente humanos, provocando cambios morfológicos duraderos de la corteza cerebral y en los hábitos mentales. Todos estos hallazgos enriquecen y confluyen en la Neurobiología Interpersonal que, al integrar conocimientos procedentes de campos muy diversos, se está revelando como una valiosa fuente de conocimientos para la práctica clínica de la psicoterapia.
La práctica de la meditación y la atención plena: técnicas milenarias para padres del siglo XXI
Authors: Santamaría Maria Teresa , Cebolla Ausiàs , Rodríguez Pedro J. , Miró María Teresa
Published: July 2021

En este trabajo se presenta un programa de entrenamiento para padres basado en la atención plena. El programa fue desarrollado en una unitad especial para el tratamiento de niños con problemas mentales graves. 18 madres participaron en el estudio, que sigue el diseño de un ensayo clínico no-aleatorizado con grupo de control. Los resultados muestran una disminución en las puntuaciones de estrés y ansiedad así como una mejora en las relaciones con los hijos.
Ion Source—Thermal and Thermomechanical Simulation
Authors: Svotina Victoria V. , Mogulkin Andrey I. , Kupreeva Alexandra Y.
Published: July 2021

The main purpose of this work is to conduct ground development testing of the ion source intended for use the space debris contactless transportation system. In order to substantiate the operating capability of the developed ion source, its thermal and thermomechanical simulation was carried out. The ion source thermal model should verify the ion source operating capability under thermal loading conditions, and demonstrate the conditions for ion source interfacing with the systems of the service spacecraft with the ion source installed as a payload. The mechanical and mathematical simulation for deformation of the ion source ion-extraction system profiled electrodes under thermal loading in conjunction with the prediction of the strained state based on the numerical simulation of the ion source ion-extraction system units, making it possible to ensure the stability of the ion source performance. Good agreement between the thermal and thermo-mechanical ion source simulation results and experimental data has been demonstrated. It is shown that the developed ion source will be functional in outer space and can be used as an element of the space debris contactless transportation system into graveyard orbits.
Pilot Study to Evaluate Performance of Frost-Yuzu Fruit Trees under Protected Cultivation
Authors: Kim Byeong-Sam , Lee Bo-Bae , Jung Seok-Kyu , Choi Hyun-Sug
Published: July 2021

This study was initiated to observe the performance of yuzu (Citrusjunos Sieb. ex Tanaka) fruit trees when affected by a late freezing in 2018 and to evaluate the recovery of frost-damaged trees during post management under protected cultivation. A—4.9 °C of minimum daily temperature and 40-day drought occurred during dormancy, which then received heavy precipitation between early- and mid-March, with 15 m s−1 more than maximum instantaneous wind speeds frequently observed. This resulted in observed decreases in height, width and volume as well as in fruiting, fruit weight and yield, as well as yield index in 60–90% defoliated yuzu trees, in addition to higher rates of shoot dieback compared to trees that experienced only 0–30% defoliation. Lower performance and recovery rates of trees grown on flat land compared to trees on sloped land were also observed. Tree and net windbreaks did not significantly affect tree vegetative growth and fruit productivity but were found to have lowered shoot mortality in 2018 and 2019. Mulch with an irrigation after freezing or foliar urea application was shown to effectively increase vegetative tree growth and fruit productivity and reduce shoot mortality.
Establishment of a Bivector Genetic Transformation System in Recalcitrant Maize Inbred Lines
Authors: Gu Yajing , Chen Xuan , Song Rentao , Qi Weiwei
Published: July 2021

Maize is an important grain crop with high nutritional value. An effective transformation system is crucial for the genetic improvement of maize traits, but many important maize inbred lines remained recalcitrant to transformation. In this study, we developed a bivector transformation system that worked well in two recalcitrant maize inbred lines. This system included an induction vector (ZmBBM-ZmWUS) and an indicator vector (GFP), using microprojectile bombardment technology combined with Agrobacterium-mediated transformation. We found that the Zheng58 and Mo17 recalcitrant inbred lines could be transformed with this system. The whole transformation cycle lasted only 52 days, 38 days less than the traditional transformation cycle. Additionally, it was possible to eliminate inference of the induction vector and obtained progenies with only the target gene. Our results suggested that the bivector system was an optimization of the current maize transformation methods and could potentially be used in genetic improvement of maize inbred lines.
Economic Returns from Cereal and Cereal/Vetch Forage Crops Grown as Fodder Conservation Options for Beef and Sheepmeat Production
Authors: Piltz John W. , Rodham Craig A. , Wilkins John F. , Hackney Belinda F. , Brown Colin G.
Published: July 2021

The economic return from cereal or cereal/vetch crops was determined using previously published and new agronomic and herbage quality data from experiments conducted at four sites across southern New South Wales, Australia, over four years (2008 to 2011), to evaluate the agronomic and quality parameters of two wheat (Triticum aestivum L.), two barley (Hordeum vulgare L.), two oat (Avena sativa L.), and one triticale (x Triticosecale) variety, grown as monocultures or in combination with purple vetch (Vicia benghalensis L.). The crops (n = 193) were harvested at different stages of cereal maturity and ranged in metabolisable energy (ME) from 6.9 to 13.1 MJ/kg DM and crude protein (CP) content from 49.8 to 215.4 g/kg DM. Individual crop ME and CP content was used to predict dry matter intake and liveweight gain using Grazfeed decision support tool, assuming the forages were fed as the sole diet to either crossbred lambs or British breed steers, with initial liveweights of 30 or 300 kg respectively. Animal parameters and yield were used to estimate gross margins (GM) for each crop based on estimated fixed and variable costs, including sowing and fertiliser costs, and harvesting and feedout costs. Feed quality determined animal production and potential income per animal, while yield determined potential income per hectare for any given level of animal production. Across the three years GM ranged from −$1489 to $5788 in sheep and from −$1764 to $647 in cattle. Reducing costs or increasing livestock value improved the GM. The highest GM were for lambs fed crops with high ME, adequate CP, and good yields. Increasing yield reduced the GM when growth rates were low, and costs exceeded the value of liveweight gain.
Update on Distribution and Conservation Status of Amphibians in the Democratic People’s Republic of Korea: Conclusions Based on Field Surveys, Environmental Modelling, Molecular Analyses and Call Properties
Authors: Borzée Amaël , Litvinchuk Spartak N. , Ri Kyongsim , Andersen Desiree , Nam Tu Yong , Jon Gwang Hyok , Man Ho Song , Choe Jong Sik , Kwon Sera , Othman Siti N. , Messenger Kevin , Bae Yoonhyuk , Shin Yucheol , Kim Ajoung , Maslova Irina , Luedtke Jennifer , Hobin Louise , Moores Nial , Seliger Bernhard , Glenk Felix , Jang Yikweon
Published: July 2021

Determining the range, status, ecology and behaviour of species from areas where surveys and samplings are uncommon or difficult to conduct is a challenge, such as in the Democratic People’s Republic of Korea (DPR Korea). Here, we used genetic samples, field surveys, call recordings, photographic identification and a literature review to estimate the presence, range and status of amphibians in the DPR Korea. From our combined results and based on the IUCN Red List categories and criteria, we were able to estimate the national threat levels for most species. Our results demonstrated the presence of 18 native species and the suspected presence of Karsenia koreana and two Onychodactylus species. We reported the first record for Rana uenoi in the vicinity of Pyongyang using molecular tools and similarly confirmed the presence of Dryophytes japonicus at the same location. Based on distribution and modelling, we can expect the contact zone between species within the Rana and Onychodactylus genera to be located along the Changbai Massif, a mountain range that marks a shift in ecoregions and acts as a barrier to dispersion. The species richness was higher in the lowlands and at lower latitudes, with such areas populated by up to 11 species, while more northern regions were characterised by species richness of about half of that value. The combination of ecological models and known threats resulted in the recommendation of ten species as threatened at the national level following the IUCN Red List categories and criteria. This high number of threatened species was anticipated based on the high threat level to amphibians in bordering nations and globally. While the ecology of species in the DPR Korea is still understudied, we argue that species relying on agricultural wetlands such as rice paddies are not under imminent threat due to the enduring presence of extensive agricultural landscapes with low rates of chemical use and mechanisation. The maintenance of such landscapes is a clear benefit to amphibian species, in contrast to more industrialised agricultural landscapes in neighbouring nations. In comparison, the status of species dependent on forested habitats is unclear and threat levels are likely to be higher because of deforestation, as in neighbouring nations.
Cardiorespiratory, Sedative and Antinociceptive Effects of a Medetomidine Constant Rate Infusion with Morphine, Ketamine or Both
Authors: Troya-Portillo Lucas , López-Sanromán Javier , Villalba-Orero María , Santiago-Llorente Isabel
Published: July 2021

Standing surgery under sedation reduces anesthetic-related mortality in horses. Medetomidine, alone and combined with morphine in a constant rate infusion (CRI), has been described for standing surgery but their cardiorespiratory, sedative and antinociceptive effects have never been compared. The addition of ketamine could improve analgesia in these procedures with minimal cardiorespiratory consequences. The objectives were to compare the cardiorespiratory effects, quality of sedation, antinociception and ataxia produced by administration of a medetomidine-based CRI with morphine, ketamine or both, in standing horses. A prospective, blind, randomized crossover, experimental design with six healthy adult horses was performed, in which four treatments were administered to all horses with at least two weeks of washout period: medetomidine (M); medetomidine and ketamine (MK); medetomidine and morphine (MMo); and medetomidine, morphine and ketamine (MMoK). Dosages were the same in all treatment groups: medetomidine at 5 µg/kg bwt followed by 5 µg/kg bwt/h, ketamine at 0.4 mg/kg/h and morphine at 50 µg/kg bwt, followed by morphine 30 µg/kg bwt/h. Drug infusions were maintained for 120 min. Cardiorespiratory variables, sedation degree and antinociceptive effects were evaluated during the procedure. All combinations produced similar sedation and antinociceptive effects and no clinically relevant alterations in cardiorespiratory variables occurred. Medetomidine CRI combined with morphine, ketamine or both are suitable and safe protocols for standing sedation in horses and the addition of morphine and/or ketamine did not cause any negative effect but no improving effect on sedation and antinociception was detected.
Oral Vaccination against Lawsoniaintracellularis Changes the Intestinal Microbiome in Weaned Piglets
Authors: Guevarra Robin B. , Cho Jae Hyoung , Cho Jin Ho , Lee Jun Hyung , Kim Hyeri , Kim Sheena , Kim Eun Sol , Keum Gi Beom , Watthanaphansak Suphot , Song Minho , Kim Hyeun Bum
Published: July 2021

Lawsoniaintracellularis, which causes porcine proliferative enteropathy (PPE), is a common swine intestinal pathogen that is prevalent in pig production sites worldwide. In this study, the alteration in the microbiome composition of weaned pigs was investigated in response to vaccination against L. intracellularis, using 16S rRNA gene sequencing. A total of 64 crossbred (Duroc × [Landrace × Yorkshire]) healthy weanling pigs weaned at 4 weeks of age were randomly assigned to four treatment groups (four pigs/pen; four pens/treatment), using a randomized complete block design for the 42-day trial. Pigs in the treatment groups were orally administered with three different doses (1 dose = 2 mL) of vaccine against L. intracellularis (Enterisol® Ileitis, Boehringer Ingelheim Vetmedica GmbH), namely the following: LAW1 (0.5 dose), LAW2 (1 dose), LAW3 (2 dose). A non-vaccinated group served as a negative control (CONT). Alpha diversity analysis revealed that vaccination led to significant changes in species evenness but not species richness of the gut microbiota. Beta diversity analysis revealed that vaccination against L. intracellularis caused a significant shift in the microbial community structure. At the genus level, there was a significant increase in Streptococcus and a significant decrease in Clostridium in the fecal microbiota of vaccinated pigs, regardless of dose.
Synovial Concentration of Trimethoprim-Sulphadiazine Following Regional Limb Perfusion in Standing Horses
Authors: Gustafsson Kajsa , Tatz Amos J. , Dahan Roee , Abu Ahmad Wiessam , Britzi Malka , Sutton Gila A. , Kelmer Gal
Published: July 2021

The aim of this study was to investigate the safety and pharmacokinetics of trimethoprim-sulphadiazine administered via intravenous regional limb perfusion (IVRLP) into the cephalic vein. According to the hypothesis, the drug could be administered without adverse effects and the synovial concentrations would remain above the minimum inhibitory concentration (MIC) for trimethoprim-sulphadiazine (0.5 and 9.5 µg/mL) for 24 h. Ten (n = 10) horses underwent cephalic vein IVRLP with an Esmarch tourniquet applied for 30 min. Four grams (4 g) of trimethoprim-sulphadiazine (TMP-SDZ) were diluted at 0.9% NaCl for a total volume of 100 mL. Synovial fluid and blood samples were obtained immediately before IVRLP and at 0.25, 0.5, 2, 6, 12 and 24 h after the initiation of IVRLP. Trimethoprim and sulphadiazine concentrations were determined using a method based on liquid chromatography/tandem mass spectrometry. The Cmax (peak drug concentration) values were 36 ± 31.1 and 275.3 ± 214.4 µg/mL (TMP and SDZ). The respective tmax (time to reach Cmax) values were 20 ± 7.8 and 26.4 ± 7.2 min. The initial synovial fluid concentrations were high but decreased quickly. No horse had synovial concentrations of trimethoprim-sulphadiazine above the MIC at 12 h. Severe vasculitis and pain shortly after IVRLP, lasting up to one week post-injection, occurred in five out of 10 horses. In conclusion, IVRLP with trimethoprim-sulphadiazine cannot be recommended due to the low concentrations of synovial fluid over time and the frequent severe adverse effects causing pain and discomfort in treated horses. Thus, in cases of septic synovitis with bacteria sensitive to trimethoprim-sulphadiazine, other routes of administration should be considered.
A Meta-Analysis to Understand the Relationship between Pig Body Weight and Variation from Birth to Market
Authors: Tolosa Andres F. , DeRouchey Joel M. , Tokach Mike D. , Goodband Robert D. , Woodworth Jason C. , Gebhardt Jordan T. , Ritter Mathew J. , Pilcher Chad M.
Published: July 2021

This meta-analysis aims to understand the changes in pig body weight (BW) variation from birth to market and develop prediction equations for coefficient of variation (CV) and standard deviation (SD) as a function of BW. Standard deviation is the measure of dispersion of a set of values from the mean and CV is the SD expressed as a percentage of the mean. Data collected from 16 papers and data sets yielded 117,268 individually weighed pigs with sample size ranging from 120 to 4108 pigs. Polynomial regression analysis was conducted separately for each variation measurement. The resulting prediction equations (CV (%) = 20.04 − 0.135 × (BW) + 0.00043 × (BW)2, R2 = 0.79; SD = 0.41 + 0.150 × (BW) − 0.00041 × (BW)2, R2 = 0.95) suggest that there is a quadratic decreasing relationship between the CV of a population and BW, the slope gets smaller as mean BW increases from birth to market. A quadratic increasing relationship is observed for SD, with slope being smaller as mean BW of pigs increases from birth to market. These prediction equations can be used by swine producers to estimate expected CV and SD of BW among a population of pigs.
Miquelianin Inhibits Allergic Responses in Mice by Suppressing CD4+ T Cell Proliferation
Authors: Choi Dae Woon , Jung Sun Young , Kim Gun-Dong , Lee So-Young , Shin Hee Soon
Published: July 2021

Allergic diseases, including atopic dermatitis (AD), induce type 2 helper T (Th2) cell-dominant immune responses. Miquelianin (quercetin 3-O-glucuronide, MQL) is an active compound in Rosae multiflorae fructus extract with anti-allergic properties. Here, we investigate the anti-allergic effects of MQL in an ovalbumin (OVA)-induced Th2-dominant mouse model and the associated mechanisms. Oral MQL suppressed cytokine and IL-2 production and proliferation of Th2 cells and upregulated heme oxygenase-1 (HO-1) in splenocytes. Ex vivo MQL suppressed Th1- and Th2-related immune responses by inhibiting CD4+ T cell proliferation, and upregulated HO-1 in CD4+ T cells by activating C-Raf–ERK1/2–Nrf2 pathway via induction of reactive oxygen species generation. In a trimellitic anhydride-induced AD-like mouse model, both topical and oral MQL ameliorated AD symptoms by suppressing Th2 immune responses. Our results suggest that MQL is a potential therapeutic agent for CD4+ T cell-mediated diseases, including allergic diseases.
Nutritional, Physiochemical, and Biological Value of Muffins Enriched with Edible Insects Flour
Authors: Zielińska Ewelina , Pankiewicz Urszula , Sujka Monika
Published: July 2021

Edible insects are gaining attention as a novel food; however, studies with their use in food are still limited. This study aimed to determine the chemical composition, physical parameters, sensory acceptance, and biological properties of muffins enriched with different levels of cricket (Gryllodes sigillatus) and mealworm (Tenebrio molitor) flours. The approximate composition was analyzed, along with the physical and textural properties, color, and consumer acceptance. Moreover, the antioxidant properties, starch digestibility, and glycemic index were determined in vitro. As we expected, the protein content in muffins supplemented with insect flour increased, while the carbohydrates content decreased. Moreover, the total phenolic content and antioxidant capacity against ABTS·+ and DPPH· increased correspondingly as the percentage of insect flour in the muffins increased. The estimated glycemic index was lower for the fortified muffins than the control (p < 0.05). Additionally, enriched muffins were accepted by consumers, and their taste positively surprised respondents (p < 0.05). Therefore, the results obtained are satisfactory as regards the use of insects for the supplementation of traditional products, and further research into the addition of insects to other nutrient matrices is needed. Furthermore, examining the effect of insect addition on in vivo food biological activity is highly desirable.
Unified Genetic Algorithm Approach for Solving Flexible Job-Shop Scheduling Problem
Authors: Park Jin-Sung , Ng Huey-Yuen , Chua Tay-Jin , Ng Yen-Ting , Kim Jun-Woo
Published: July 2021

This paper proposes a novel genetic algorithm (GA) approach that utilizes a multichromosome to solve the flexible job-shop scheduling problem (FJSP), which involves two kinds of decisions: machine selection and operation sequencing. Typically, the former is represented by a string of categorical values, whereas the latter forms a sequence of operations. Consequently, the chromosome of conventional GAs for solving FJSP consists of a categorical part and a sequential part. Since these two parts are different from each other, different kinds of genetic operators are required to solve the FJSP using conventional GAs. In contrast, this paper proposes a unified GA approach that enables the application of an identical crossover strategy in both the categorical and sequential parts. In order to implement the unified approach, the sequential part is evolved by applying a candidate order-based GA (COGA), which can use traditional crossover strategies such as one-point or two-point crossovers. Such crossover strategies can also be used to evolve the categorical part. Thus, we can handle the categorical and sequential parts in an identical manner if identical crossover points are used for both. In this study, the unified approach was used to extend the existing COGA to a unified COGA (u-COGA), which can be used to solve FJSPs. Numerical experiments reveal that the u-COGA is useful for solving FJSPs with complex structures.
Characterization with X-rays of a Large-Area GEMPix Detector with Optical Readout for QA in Hadron Therapy
Authors: Maia Oliveira Andreia , Akkerman Hylke B. , Braccini Saverio , van Breemen Albert J. J. M. , Gallego Manzano Lucia , Heracleous Natalie , Katsouras Ilias , Leidner Johannes , Murtas Fabrizio , Peeters Bart , Silari Marco
Published: July 2021

Quality Assurance (QA) in hadron therapy is crucial to ensure safe and accurate dose delivery to patients. This can be achieved with fast, reliable and high-resolution detectors. In this paper, we present a novel solution that combines a triple Gas Electron Multiplier (GEM) and a highly pixelated readout based on a matrix of organic photodiodes fabricated on top of an oxide-based thin-film transistor backplane. The first LaGEMPix prototype with an active area of 60 × 80 mm2 was developed and characterized using low energy X-rays. The detector comprises a drift gap of 3.5 mm, a triple-GEM stack for electron amplification, and a readout featuring 480 × 640 pixels at a 126 µm pitch. Here, we describe the measurements and results in terms of spatial resolution for various experimental configurations. A comparison with GAFCHROMIC® films and the GEMPix detector used in the charge readout mode was performed to better understand the contribution to the spatial resolution from both the electron diffusion and the isotropic emission of photons. The measurements were compared to Monte Carlo simulations, using the FLUKA code. The simulation predictions are in good agreement with the GEMPix results. Future plans with respect to applications in hadron therapy are discussed.
Modelling the Microphone-Related Timbral Brightness of Recorded Signals
Authors: Pearce Andy , Brookes Tim , Mason Russell
Published: July 2021

Brightness is one of the most common timbral descriptors used for searching audio databases, and is also the timbral attribute of recorded sound that is most affected by microphone choice, making a brightness prediction model desirable for automatic metadata generation. A model, sensitive to microphone-related as well as source-related brightness, was developed based on a novel combination of the spectral centroid and the ratio of the total magnitude of the signal above 500 Hz to that of the full signal. This model performed well on training data (r = 0.922). Validating it on new data showed a slight gradient error but good linear correlation across source types and overall (r = 0.955). On both training and validation data, the new model out-performed metrics previously used for brightness prediction.
Reliability-Based Design of an Aircraft Wing Using a Fuzzy-Based Metaheuristic
Authors: Winyangkul Seksan , Sleesongsom Suwin , Bureerat Sujin
Published: July 2021

The purpose of this paper is to design aircraft wing using reliability-based design optimization concerned to fuzzy uncertainty variables. A possibilistic safety index-based design optimization (PSIBDO) with fuzzy uncertainties is proposed to overcome difficult tasks from the original probabilistic problem. The design problem is to minimize mass of a composite aircraft wing subject to aeroelastic and structural constraints through consideration of the material properties are the uncertainties. The design variables include aircraft wing structure dimensions. The reliability-based design approach is needed to alleviate such a problem. Due to the complexity of the aircraft wing structures design and aeroelastic analysis, nonprobability-based design is an alternative choice to increase computational efficiency in the design process. The optimum results show the efficiency of our proposed approach.
Extracting SBVR Business Vocabularies from UML Use Case Models Using M2M Transformations Based on Drag-and-Drop Actions
Authors: Skersys Tomas , Danenas Paulius , Butleris Rimantas , Ostreika Armantas , Ceponis Jonas
Published: July 2021

In the domain of model-driven system engineering, model-to-model (M2M) transformations present a very relevant topic because they may provide much-needed automation capabilities to the whole CASE-supported system development life cycle. Nonetheless, it is observed that throughout the whole development process M2M transformations are spread unevenly; in this respect, the phases of Business Modeling and System Analysis are arguably the most underdeveloped ones. The main novelty and contributions of this paper are the presented set of model-based transformations for extracting well-structured SBVR business vocabularies from visual UML use case models, which utilizes M2M transformation technology based on the so-called drag-and-drop actions. The conducted experiments show that this new development provides the same transformation power while introducing more flexibility to the model development process as compared to our previously developed approach for (semi-)automatic extraction of SBVR business vocabularies from UML use case models.
Sliding Mode-Based Slip Control of Compact Electric Vehicle Truck for Varying Load and Yaw Rate
Authors: Kim Hyeon-Woo , Cha Hyun-Rok
Published: July 2021

Vehicle stability is a critical problem, especially for compact electric vehicle (EV) trucks, owing to the impact of the cargo weight and cornering characteristics. In this study, this problem was approached by mathematically formulating the change in the understeer characteristics of an EV truck as variable mass understeer gradient (VMUG) according to the vehicle cargo weight to design the reference yaw rate without the need to consider cornering stiffness. Comparison was made with the conventional methods by applying the VMUG-based slip control while simulating the yaw rate and side-slip tracking performance of the compact EV model for normal loading and overloading conditions. The simulation results demonstrate the superior performance of the proposed method compared to the existing methods. The proposed method has the potential for application for stability enhancement in non-electric and general-purpose vehicles as well.
Natural Radioactivity, Radiological Hazard and Petrographical Studies on Aswan Granites Used as Building Materials in Egypt
Authors: Moghazy Nasser M. , El-Tohamy Amira M. , Fawzy Mona M. , Awad Hamdy A. , Zakaly Hesham M. H. , Issa Shams A. M. , Ene Antoaneta
Published: July 2021

The present study was carried out on commercial types of Aswan granite used as building and decorative materials. Nearly 29 granitic rocks samples from 11 classes (black Aswan, red Aswan, dark Rosa, light Rosa, yellow Verdi, grey Shirka, Gandolla, Forsan, red Nefertiti, Royal, and white Halayeb) were collected from three stations near Aswan city for petrographical description and assessment of natural radioactivity. The petrographical study of granites was conducted by polarized-light microscope in order to determine their mineralogical composition and investigate their texture; the activity of the natural radionuclides 238U, Ra226, 232Th, and 40K was measured by gamma-ray spectrometry with a NaI(Tl) detector. The average values of the activities, 52.2 Bq kg−1, 57.8 Bq kg−1, 31.2 Bq kg−1, and 1055.7 Bq kg−1 of U-238, Th-232, Ra-226, and K-40, respectively, were higher than that the world average values of 35 Bq kg−1, 30 Bq kg−1 and 400 Bq kg−1 for 226Ra, 232Th, and 40K, respectively, according to the recommended levels from UNSCEAR reports. The minimum and maximum values obtained were compared with the value ranges from other locations in the Eastern Desert, highlighting the fact that that the maximum values obtained in this work are higher than those in other areas. According to the radiological hazards indices results, most samples lie in the permissible level ranges, suggesting their favorability for use as building materials. In contrast to that, some samples have some environmental parameters higher than the international levels, indicating their unsuitability as building materials.
The Role of Veracity on the Load Monitoring of Professional Soccer Players: A Systematic Review in the Face of the Big Data Era
Authors: Claudino João Gustavo , Cardoso Filho Carlos Alberto , Boullosa Daniel , Lima-Alves Adriano , Carrion Gustavo Rejano , GianonI Rodrigo Luiz da Silva , Guimarães Rodrigo dos Santos , Ventura Fúlvio Martins , Araujo André Luiz Costa , Del Rosso Sebastián , Afonso José , Serrão Julio Cerca
Published: July 2021

Big Data has real value when the veracity of the collected data has been previously identified. However, data veracity for load monitoring in professional soccer players has not been analyzed yet. This systematic review aims to evaluate the current evidence from the scientific literature related to data veracity for load monitoring in professional soccer. Systematic searches through the PubMed, Scopus, and Web of Science databases were conducted for reports onthe data veracity of diverse load monitoring tools and the associated parameters used in professional soccer. Ninety-four studies were finally included in the review, with 39 different tools used and 578 associated parameters identified. The pooled sample consisted of 2066 footballers (95% male: 24 ± 3 years and 5% female: 24 ± 1 years). Seventy-three percent of these studies did not report veracity metrics for anyof the parameters from these tools. Thus, data veracity was found for 54% of tools and 23% of parameters. The current information will assist in the selection of the most appropriate tools and parameters to be used for load monitoring with traditional and Big Data approaches while identifying those still requiring the analysis of their veracity metrics or their improvement to acceptable veracity levels.
Fast Segmentation Algorithm for Cystoid Macular Edema Based on Omnidirectional Wave Operator
Authors: Liu Jing , Lou Shiliang , Chen Xiaodong , Cai Huaiyu , Wang Yi
Published: July 2021

Optical coherence tomography (OCT) is widely used in the field of ophthalmic imaging. The existing technology cannot automatically extract the contour of the OCT images of cystoid macular edema (CME) and can only evaluate the degree of lesions by detecting the thickness of the retina. To solve this problem, this paper proposes an automatic segmentation algorithm that can segment the CME in OCT images of the fundus quickly and accurately. This method firstly constructs the working environment by denoising and contrast stretching, secondly extracts the region of interest (ROI) containing CME according to the average gray distribution of the image, and then uses the omnidirectional wave operator to perform multidirectional automatic segmentation. Finally, the fused segmentation results are screened by gray threshold and position feature, and the contour extraction of CME is realized. The segmentation results of the proposed method on data set images are compared with those obtained by manual marking of experts. The accuracy, recall, Dice index, and F1-score are 88.8%, 75.0%, 81.1%, and 81.3%, respectively, with the average process time being 1.2 s. This algorithm is suitable for general CME image segmentation and has high robustness and segmentation accuracy.
Compilation and Evaluation of Ambient Respirable Crystalline Silica Air Quality Data near Sand Quarries and Processing Facilities
Authors: Richards John , Brozell Todd
Published: July 2021

Ambient respirable crystalline silica air quality is of concern to many communities near mineral processing facilities and to regulatory agencies serving these communities. Accurate air quality data are needed to compare measured respirable crystalline silica concentrations at the fencelines of mineral processing facilities with the published health effect guideline published by the California Office of Health Hazard Assessment (OEHHA). This article is a compilation and evaluation of air quality studies around a diverse set of nineteen sand producing facilities. The respirable crystalline silica air quality data compiled by Air Control Techniques, P.C. and most of the data compiled by other researchers cited in this article have been measured using EPA Reference Method samplers adjusted for respirable crystalline silica sampling and NIOSH Method 7500 X-ray diffraction analyses. The authors conclude that (1) the ambient concentrations in the diverse set of mineral processing facilities were consistently lower than the 3.0 microgram per cubic meter chronic reference exposure level (REL) adopted by OEHHA, (2) upwind-to-downwind fenceline concentration differences were small, and (3) the fenceline t concentrations were often at background concentration levels. The authors recommend additional sampling studies to better characterize background concentrations of ambient respirable crystalline silica.
The ICON Single-Column Mode
Authors: Bašták Ďurán Ivan , Köhler Martin , Eichhorn-Müller Astrid , Maurer Vera , Schmidli Juerg , Schomburg Annika , Klocke Daniel , Göcke Tobias , Schäfer Sophia , Schlemmer Linda , Dewani Noviana
Published: July 2021

The single-column mode (SCM) of the ICON (ICOsahedral Nonhydrostatic) modeling framework is presented. The primary purpose of the ICON SCM is to use it as a tool for research, model evaluation and development. Thanks to the simplified geometry of the ICON SCM, various aspects of the ICON model, in particular the model physics, can be studied in a well-controlled environment. Additionally, the ICON SCM has a reduced computational cost and a low data storage demand. The ICON SCM can be utilized for idealized cases—several well-established cases are already included—or for semi-realistic cases based on analyses or model forecasts. As the case setup is defined by a single NetCDF file, new cases can be prepared easily by the modification of this file. We demonstrate the usage of the ICON SCM for different idealized cases such as shallow convection, stratocumulus clouds, and radiative transfer. Additionally, the ICON SCM is tested for a semi-realistic case together with an equivalent three-dimensional setup and the large eddy simulation mode of ICON. Such consistent comparisons across the hierarchy of ICON configurations are very helpful for model development. The ICON SCM will be implemented into the operational ICON model and will serve as an additional tool for advancing the development of the ICON model.
Evaluation of Tourism-Climate Conditions in the Region of Kłodzko Land (Poland)
Authors: Miszuk Bartłomiej
Published: July 2021

Kłodzko Land is one of the most important regions of Poland in terms of tourism and health issues. Numerous tourism attractions and health resorts make the region attractive for both tourist and bathers. The goal of this paper was to evaluate the impact of weather conditions on tourism-related conditions and their changes in the multiannual period. In the analysis, the indices of heat days, the UTCI (Universal Thermal Climate Index) and CTIS (Climate Tourism Information Scheme) tools were used. The research on heat days and the UTCI indicated a significant increase in the heat-stress frequency over the last decades. Simultaneously, the number of weather types related to cold stress has considerably decreased. Such trends were noticed in the entire region, in all the considered hypsometric zones. The rising tendency was also observed for strong and very strong heat stress (UTCI > 32 °C), which negatively affects health problems. The analysis showed that the most extreme thermal and biothermal conditions, in terms of heat stress, occur under southern and eastern anticyclonic circulation. The CTIS analysis showed that favorable weather conditions for most of tourism activities are noticed in the warm half-year. The usefulness of weather conditions for tourism can vary depending on atmospheric circulation.
A New Approach for the Analysis of Deep Convective Events: Thunderstorm Intensity Index
Authors: Jelić Damjan , Telišman Prtenjak Maja , Malečić Barbara , Belušić Vozila Andreina , Megyeri Otília Anna , Renko Tanja
Published: July 2021

In this study, an investigation of a new thunderstorm intensity index (TSII) derived from lightning data is performed, along with its relationship to rain, wind, hail and waterspouts as well as instability indices (CAPE, LI, KI, and DLS). The study area is located in the northeastern Adriatic and includes various terrain types in a relatively small area (coastal, flatlands, hills and valleys, and mountain regions). The investigated period covers 11 years (2008–2018). The mathematical algorithm standing behind the TSII is based on the well-established methodology of lightning jump, allowing us to recognize areas where intensification in thunderstorms occurred. Our results suggest that these areas (with a positive TSII) experience significantly higher rain intensities and have higher total amounts of precipitation compared with areas where thunderstorms did not generate a TSII. Moreover, 76% of thunderstorm hail cases were associated with the presence of a TSII within a 15 km distance. The maximum reported wind speed also has higher values on a day with a TSII. Out of 27 waterspout events associated with lightning, 77% were related to a TSII. Due to the good spatial (3 km × 3 km) and high temporal (2 min) resolution of lightning data, the TSII can recognize even a local and short-lived intense system that is often misread by radars and satellites due to their inferior temporal resolution. The TSII is designed to be used as a climatological and diagnostic variable that could serve in lieu of more established data sources (e.g., station measurements and observations, radar imagery, etc.) if they are unavailable.
Targeted Lipidomic Analysis of Aqueous Humor Reveals Signaling Lipid-Mediated Pathways in Primary Open-Angle Glaucoma
Authors: Azbukina Nadezhda V. , Chistyakov Dmitry V. , Goriainov Sergei V. , Kotelin Vladislav I. , Fedoseeva Elena V. , Petrov Sergey Yu. , Sergeeva Marina G. , Iomdina Elena N. , Zernii Evgeni Yu.
Published: July 2021

Primary open-angle glaucoma (POAG) is characterized by degeneration of retinal ganglion cells associated with an increase in intraocular pressure (IOP) due to hindered aqueous humor (AH) drainage through the trabecular meshwork and uveoscleral pathway. Polyunsaturated fatty acids and oxylipins are signaling lipids regulating neuroinflammation, neuronal survival and AH outflow. Among them, prostaglandins have been previously implicated in glaucoma and employed for its treatment. This study addressed the role of signaling lipids in glaucoma by determining their changes in AH accompanying IOP growth and progression of the disease. Eye liquids were collected from patients with POAG of different stages and cataract patients without glaucoma. Lipids were identified and quantified by UPLC-MS/MS. The compounds discriminating glaucoma groups were recognized using ANCOVA and PLS-DA statistic approaches and their biosynthetic pathways were predicted by bioinformatics. Among 22 signaling lipids identified in AH, stage/IOP-dependent alterations in glaucoma were provided by a small set of mediators, including 12,13-DiHOME, 9- and 13-HODE/KODE, arachidonic acid and lyso-PAF. These observations correlated with the expression of cytochromes P450 (CYPs) and phospholipases A2 in the ocular tissues. Interestingly, tear fluid exhibited similar lipidomic alterations in POAG. Overall, POAG may involve arachidonic acid/PAF-dependent pathways and oxidative stress as evidenced from an increase in its markers, KODEs and 12,13-DiHOME. The latter is a product of CYPs, one of which, CYP1B1, is known as POAG and primary congenital glaucoma-associated gene. These data provide novel targets for glaucoma treatment. Oxylipin content of tear fluid may have diagnostic value in POAG.
TLR7 Activation of Macrophages by Imiquimod Inhibits HIV Infection through Modulation of Viral Entry Cellular Factors
Authors: Meng Feng-Zhen , Liu Jin-Biao , Wang Xu , Wang Peng , Hu Wen-Hui , Hou Wei , Ho Wen-Zhe
Published: July 2021

The Toll-like receptor (TLR) 7 is a viral sensor for detecting single-stranded ribonucleic acid (ssRNA), the activation of which can induce intracellular innate immunity against viral infections. Imiquimod, a synthetic ligand for TLR7, has been successfully used for the topical treatment of genital/perianal warts in immunocompetent individuals. We studied the effect of imiquimod on the human immunodeficiency virus (HIV) infection of primary human macrophages and demonstrated that the treatment of cells with imiquimod effectively inhibited infection with multiple strains (Bal, YU2, and Jago) of HIV. This anti-HIV activity of imiquimod was the most potent when macrophages were treated prior to infection. Infection of macrophages with pseudotyped HIV NL4-3-ΔEnv-eGFP-Bal showed that imiquimod could block the viral entry. Further mechanistic studies revealed that while imiquimod had little effect on the interferons (IFNs) expression, its treatment of macrophages resulted in the increased production of the CC chemokines (human macrophage inflammatory protein-1 alpha (MIP-1α), MIP-1β, and upon activation regulated normal T cells expressed and secreted (RANTES)), the natural ligands of HIV entry co-receptor CCR5, and decreased the expression of CD4 and CCR5. The addition of the antibodies against the CC chemokines to macrophage cultures could block imiquimod-mediated HIV inhibition. These findings provide experimental evidence to support the notion that TLR7 participates in the intracellular immunity against HIV in macrophages, suggesting the further clinical evaluation of imiquimod for its additional benefit of treating genital/perianal warts in people infected with HIV.
Descriptive Study about Bodyweight Status of Extremadura Adolescents. Are We Applying the Best Indicator as the Reference Parameter?
Authors: Mendoza-Muñoz María , Muñoz-Bermejo Laura , Gómez-Galán Rafael , Calle-Guisado Violeta , Pastor-Cisneros Raquel , Garcia-Gordillo Miguel Ángel , Adsuar José Carmelo , Carlos-Vivas Jorge
Published: July 2021

Background: There is no agreed criteria that establishes childhood obesity thresholds based on BMI, which may be used to assess adolescent overweight/obesity. This tool has been determined at the most practical and least costly in classifying bodyweight status in adolescents. However, it is an indicator of bodyweight and not adiposity. Aims: To assess bodyweight status of Extremadura adolescents by sex and age using international, national, and regional reference criteria and comparing the different diagnoses criteria. Methods: A descriptive cross-sectional study was conducted with 4130 adolescents (12–17 years). Bodyweight and height were assessed. Results: Pairwise comparisons indicates that the World Health Organization (WHO) classified 542 individuals in a different category compared to Faustino Obergozo (FO), and 1028 individuals with respect to the Extremadura adolescents’ percentiles (EX). Moreover, FO classified 684 adolescents in a different category than EX. Despite the concordance in diagnostic criteria (by Cohen’s kappa test) reported between the WHO, FO, and EX for all bodyweight categories in both sexes and all age ranges, significant differences were found (assessed by Cochran Q test and McNemar test as post-hoc) between the WHO and FO for all bodyweight proportion except in the thinness category in girls (15–17 years) and boys (12–14 years). Meaningful differences were also obtained comparing WHO and EX for each bodyweight category in all ages and sexes. Comparisons between FO and EX revealed significant differences for all bodyweight categories in all participants except for overweight in girls (12–14 years) and boys (15–17 years) and normal weight and obesity in girls (15–17 years). Conclusions: the WHO, FO, and EX criteria present different outcomes estimating overweight and/or obesity prevalence in adolescents aged between 12 and 17 years. The change from Extremadura criteria to the WHO reference will result in more adolescents being diagnosed as overweight or obese.