bluuebunny/crossref_metadata_2025 huggingface dataset:
Assembly of α-synuclein and neurodegeneration in the central nervous system of heterozygous M83 mice following the peripheral administration of α-synuclein seeds
Authors: Macdonald Jennifer A. , Chen John L. , Masuda-Suzukake Masami , Schweighauser Manuel , Jaunmuktane Zane , Warner Thomas , Holton Janice L. , Grossman Annabelle , Berks Richard , Lavenir Isabelle , Goedert Michel
Published: November 2021

Peripheral administration (oral, intranasal, intraperitoneal, intravenous) of assembled A53T α-synuclein induced synucleinopathy in heterozygous mice transgenic for human mutant A53T α-synuclein (line M83). The same was the case when cerebellar extracts from a case of multiple system atrophy with type II α-synuclein filaments were administered intraperitoneally, intravenously or intramuscularly. We observed abundant immunoreactivity for pS129 α-synuclein in nerve cells and severe motor impairment, resulting in hindlimb paralysis and shortened lifespan. Filaments immunoreactive for pS129 α-synuclein were in evidence. A 70% loss of motor neurons was present five months after an intraperitoneal injection of assembled A53T α-synuclein or cerebellar extract with type II α-synuclein filaments from an individual with a neuropathologically confirmed diagnosis of multiple system atrophy. Microglial cells changed from a predominantly ramified to a dystrophic appearance. Taken together, these findings establish a close relationship between the formation of α-synuclein inclusions in nerve cells and neurodegeneration, accompanied by a shift in microglial cell morphology. Propagation of α-synuclein inclusions depended on the characteristics of both seeds and transgenically expressed protein.
No patella resurfacing total knee arthroplasty leads to reduction in the thickness of patellar cartilage to less than half within 5 years: a quantitative longitudinal evaluation using MRI
Authors: Sato Dai , Inoue Masayuki , Sasaki Takuro , Uchida Jun , Onodera Tomohiro , Kondo Eiji , Iwasaki Norimasa
Published: November 2021

Purpose

Patellar resurfacing in total knee arthroplasty (TKA) remains controversial as recent meta-analyses have not shown its clear superiority; however, most authors recommend it because it is associated with less frequent anterior knee pain and need for reoperation. We aimed to clarify the changes in patellar cartilage thickness in no patellar resurfacing TKA using a ceramic femoral component on magnetic resonance imaging (MRI).

Methods

Between 2009 and 2014, 40 consecutive patients (59 knees) were included in this study. All patients underwent TKA using zirconia ceramic femoral implants without patellar resurfacing. Indications for no patellar resurfacing TKA were absence of anterior knee pain, patellar compression pain, and osteoarthritic changes in the patellofemoral joint on plain radiography. The mean postoperative follow-up duration was 81.5 months (range, 25–131 months). Clinical and radiological evaluations were performed preoperatively and 5 years after TKA. Patellar cartilage thickness was evaluated preoperatively and every year for 5 years after TKA using MRI T2-weighted imaging. The patellar cartilage was divided into three regions of interest: medial, central, and lateral. To standardise the variation in patellar thickness among patients, the percent cartilage thickness was calculated.

Results

The implant’s position was appropriate in all cases. Compared to preoperative scores, 5 years postoperatively, the Japanese Orthopedic Association score and Oxford knee score significantly improved from 52.1 to 84.7; mean tilting angle and congruence angle did not change significantly; mean lateral shift ratio significantly increased from 7.1% to 14.6%; cartilage thickness significantly decreased (P < 0.05); and the percentage cartilage thickness of the central, medial, and lateral cartilage zones gradually thinned to less than half. Four patients underwent conversion to patellar resurfacing due to anterior knee pain, without loosening the femoral and tibial implants.

Conclusion

The patellar cartilage thickness decreased to less than half its preoperative level within 5 years after no patellar resurfacing TKA; this would led to clinical problems and conversion to patellar resurfacing.

Level of evidence

Level III.
Effects of therapeutic plasma exchange on the endothelial glycocalyx in septic shock
Authors: Stahl Klaus , Hillebrand Uta Carola , Kiyan Yulia , Seeliger Benjamin , Schmidt Julius J. , Schenk Heiko , Pape Thorben , Schmidt Bernhard M. W. , Welte Tobias , Hoeper Marius M. , Sauer Agnes , Wygrecka Malgorzata , Bode Christian , Wedemeyer Heiner , Haller Hermann , David Sascha
Published: November 2021

Background

Disruption of the endothelial glycocalyx (eGC) is observed in septic patients and its injury is associated with multiple-organ failure and inferior outcomes. Besides this biomarker function, increased blood concentrations of shedded eGC constituents might play a mechanistic role in septic organ failure. We hypothesized that therapeutic plasma exchange (TPE) using fresh frozen plasma might influence eGC-related pathology by removing injurious mediators of eGC breakdown while at the time replacing eGC protective factors.

Methods

We enrolled 20 norepinephrine-dependent (NE > 0.4 μg/kg/min) patients with early septic shock (onset < 12 h). Sublingual assessment of the eGC via sublingual sidestream darkfield (SDF) imaging was performed. Plasma eGC degradation products, such as heparan sulfate (HS) and the eGC-regulating enzymes, heparanase (Hpa)-1 and Hpa-2, were obtained before and after TPE. A 3D microfluidic flow assay was performed to examine the effect of TPE on eGC ex vivo. Results were compared to healthy controls.

Results

SDF demonstrated a decrease in eGC thickness in septic patients compared to healthy individuals (p = 0.001). Circulating HS levels were increased more than sixfold compared to controls and decreased significantly following TPE [controls: 16.9 (8–18.6) vs. septic patients before TPE: 105.8 (30.8–143.4) μg/ml, p < 0.001; vs. after TPE: 70.7 (36.9–109.5) μg/ml, p < 0.001]. The Hpa-2 /Hpa-1 ratio was reduced in septic patients before TPE but normalized after TPE [controls: 13.6 (6.2–21.2) vs. septic patients at inclusion: 2.9 (2.1–5.7), p = 0.001; vs. septic patients after TPE: 13.2 (11.2–31.8), p < 0.001]. Ex vivo stimulation of endothelial cells with serum from a septic patient induced eGC damage that could be attenuated with serum from the same patient following TPE.

Conclusions

Septic shock results in profound degradation of the eGC and an acquired deficiency of the protective regulator Hpa-2. TPE removed potentially injurious eGC degradation products and partially attenuated Hpa-2 deficiency.

Trial registration clinicaltrials.gov NCT04231994, retrospectively registered 18 January 2020
Changes in surface water masses in the northern East China Sea since the Last Glacial Maximum based on diatom assemblages
Authors: Shirota Keiko , Okazaki Yusuke , Konno Susumu , Miyairi Yosuke , Yokoyama Yusuke , Kubota Yoshimi
Published: November 2021

We investigated diatom assemblages for the last 19 ka in a piston core KY07-04 PC01 collected from the northern Okinawa Trough, East China Sea. Most of the diatom taxa in the Core KY07-04 PC01 were subtropical to temperate marine species throughout the core. Pronounced turnover of major diatom groups from periphytic to planktonic taxa occurred during deglaciation between 17 and 14 ka ago. During the Last Glacial Maximum (LGM) to early deglaciation, the representative taxa were Paralia spp., a periphytic diatom abundant in continental shelves of the modern East China Sea covering the Chinese Coastal Water. Relative abundances of Paralia spp. rapidly decreased during 15–14 ka ago, corresponding to sea-level rise, displaced by planktonic taxa such as Thalassionema. Thalassionema nitzschioides varieties living in subtropical water increased since 12 ka and have reached the present level at 8 ka ago, suggesting strengthening the influences of Kuroshio water in the northern East China Sea during the early to middle Holocene. Warm planktonic diatom taxa such as Nitzschia bicapitata also gradually increased from 12 to 8 ka ago. Near the core top, a sudden increase in N. bicapitata might relate to the intensifying Kuroshio during the latest Holocene.
Global, regional, and national burden of kidney, bladder, and prostate cancers and their attributable risk factors, 1990–2019
Authors: Zi Hao , He Shao-Hua , Leng Xie-Yuan , Xu Xiao-Feng , Huang Qiao , Weng Hong , Zhu Cong , Li Lu-Yao , Gu Jia-Min , Li Xu-Hui , Ming Dao-Jing , Li Xiao-Dong , Yuan Shuai , Wang Xing-Huan , He Da-Lin , Zeng Xian-Tao
Published: November 2021

Background

The burden of kidney, bladder, and prostate cancers has changed in recent decades. This study aims to investigate the global and regional burden of, and attributable risk factors for genitourinary cancers during the past 30 years.

Methods

We extracted data of kidney, bladder, and prostate cancers from the Global Burden of Disease 2019 database, including incidence, mortality, disability-adjusted life-years (DALYs), and attributable risk factors from 1990 to 2019. Estimated annual percentage changes (EAPC) were calculated to assess the changes in age-standardized incidence rate, age-standardized mortality rate (ASMR), and age-standardized DALYs rate (ASDR). The associations between cancers burden and socio-demographic index (SDI) were also analyzed.

Results

Compared with 1990, the global incident cases in 2019 were higher by 154.78%, 123.34%, and 169.11% for kidney, bladder, and prostate cancers, respectively. During the 30-year study period, there was a downward trend in ASMR and ASDR for bladder cancer (EAPC = − 0.68 and − 0.83, respectively) and prostate cancer (EAPC = − 0.75 and − 0.71, respectively), but an upward trend for kidney cancer (EAPC = 0.35 and 0.12, respectively). Regions and countries with higher SDI had higher incidence, mortality, and DALYs for all three types of cancers. The burden of bladder and prostate cancers was mainly distributed among older men, whereas the burden of kidney cancer increased among middle-aged men. Smoking related mortality and DALYs decreased, but high body mass index (BMI) and high fasting plasma glucose (FPG) related mortality and DALYs increased among kidney, bladder, and prostate cancers during the study period.

Conclusions

Kidney, bladder, and prostate cancers remain major global public health challenges, but with distinct trend for different disease entity across different regions and socioeconomic status. More proactive intervention strategies, at both the administrative and academic levels, based on the dynamic changes, are needed.
Evaluation of Sedentary Behavior and Physical Activity Levels Using Different Accelerometry Protocols in Children from the GENOBOX Study
Authors: Llorente-Cantarero Francisco Jesus , Jurado-Castro Jose Manuel , Leis Rosaura , Vázquez-Cobela Rocío , González-Gil Esther M. , Aguilera Concepción María , Bueno Gloria , Moreno Luis A. , Gil Angel , Gil-Campos Mercedes
Published: November 2021

Background

Physical activity (PA) has acquired a significant relevance due to the health benefits associated with its practice. Accelerometers are an effective tool to assess PA; however, the diversity of cut-off points used to define different PA intensities through accelerometry could interfere in the interpretation of the findings among studies.

Objectives

The present study aimed to examine the sedentary behavior (SB) and physical activity (PA) levels in children using six selected accelerometry protocols based on diverse cut-off points.

Methods

Clinical examination, anthropometric measurements, and PA evaluation by accelerometry were assessed in 543 selected children (10 ± 2.4 years old) from the Spanish GENOBOX study. The ActiLife data scoring program was used to determine daily min spent in SB, and light, moderate, vigorous and moderate-vigorous PA using six validated accelerometry protocols differing in their cut-off points.

Results

Very different estimations for SB and PA intensity levels were found in children, independently of the non-wear-time algorithm selected, and considering puberty stages, age and body mass index. The time spent in daily SB varied from 471 to 663.7 min, PA ranged from 141 to 301.6 min, and the moderate-vigorous PA was reported between 20.7 and 180.2 min.

Conclusion

The choice of a particular accelerometry protocol considering these factors is important to evaluate SB or PA intensities to suit the characteristics of the sample researched. It seems necessary to establish future lines of research that include different analytical approaches to measure SB and PA by accelerometry based on standardized and validated methodology.
Effect of bioactive glass addition on the physical properties of mineral trioxide aggregate
Authors: Kim Jei , Kim Hyun-Jung , Chang Seok Woo , Oh Soram , Kim Sun-Young , Choi Kyoung-Kyu , Kim Duck-Su , Jang Ji-Hyun
Published: November 2021

Background

The addition of bioactive glass (BG), a highly bioactive material with remineralization potential, might improve the drawback of weakening property of mineral trioxide aggregates (MTA) when it encounters with body fluid. This study aims to evaluate the effect of BG addition on physical properties of MTA.

Methods

ProRoot (MTA), and MTA with various concentrations of BG (1, 2, 5 and 10% BG/MTA) were prepared. Simulated body fluid (SBF) was used to investigate the effect of the storage solution on dentin remineralization. Prepared specimens were examined as following; the push-out bond strength to dentin, compressive strength, setting time solubility and X-ray diffraction (XRD) analysis.

Results

The 2% BG/MTA showed higher push-out bond strengths than control group after 7 days of SBF storage. The 2% BG/MTA exhibited the highest compressive strength. Setting times were reduced in the 1 and 2% BG/MTA groups, and solubility of all experimental groups were clinically acceptable. In all groups, precipitates were observed in dentinal tubules via SEM. XRD showed the increased hydroxyapatite peaks in the 2, 5 and 10% BG/MTA groups.

Conclusion

It was verified that the BG-added MTA increased dentin push-out bond strength and compressive strength under SBF storage. The addition of BG did not negatively affect the MTA maturation reaction; it increased the amount of hydroxyapatite during SBF maturation.
Challenges to the implementation of in situ simulation at HEMS bases: a qualitative study of facilitators’ expectations and strategies
Authors: Bredmose Per P. , Østergaard Doris , Sollid Stephen
Published: November 2021

Introduction

Facilitators play an essential role in simulation-based training on helicopter emergency medical services (HEMS) bases. There is scant literature about the barriers to the implementation of simulation training in HEMS. The purpose of this explorative interview study was to identify factors that the local facilitators anticipated would challenge the smooth implementation of the program, and their strategies to overcome these before the national implementation of in situ simulation-based training locally, and subsequently, one year after the programme was initiated, to identify the actual challenges they had indeed experienced, and their solutions to overcome these.

Methods

A qualitative study with semi-structured group interviews of facilitators was undertaken before and after one year of simulation-based training on all HEMS bases and one Search and Rescue base. Systematic text condensation was used to extract facilitators’ expectations and experiences.

Results

Facilitators identified 17 themes in the pre-study-year interviews. Pedagogical, motivational and logistical issues were amongst the dominant themes. Other key themes included management support, dedicated time for the facilitators and ongoing development of the facilitator. In the post-study-year interviews, the same themes were identified. Despite anxiety about the perceptions of, and enthusiasm for, simulation training amongst the HEMS crews, our facilitators describe increasing levels of motivation over the study period.

Conclusion

Facilitators prognosticated the anticipated challenges to the successful implementation of simulation-based training on HEMS bases and suggested solutions for overcoming these challenges. After one year of simulation-based training, the facilitators reflected on the key factors for successful implementation.
Age estimation with cemental incremental lines in normal and periodontally diseased teeth using phase contrast microscope: an original research
Authors: Sultana Ameena , Zainab Heena , Jahagirdar Pramod , Hugar Deepa , Shaimaa
Published: November 2021

Background

Age estimation is an important factor in forensic science for human identification. Teeth are considered to play a vital role as they resist decomposition at death unlike other tissues. This resistance and the gradual structural changes that take place throughout the life of an individual have made teeth useful indicators for age estimation. Dental cementum shows continuous apposition throughout the life of an individual. Tooth cemental annulation is a microscopic method for the determination of an individual’s age based on the analysis of incremental lines of cementum. Light microscopy as well as specialized microscopic methods have been employed to enhance the assessment of the cemental annulations. Periodontal disease is the most common dental problem affecting millions of people. Assessing the efficiency of the tooth cemental annulations method in periodontally diseased teeth is an important requisite. This study aims at assessing and evaluating the tooth cemental annulations in normal and periodontally diseased teeth using phase contrast microscopic method for age determination.

Results

A total of 60 teeth were included in the study and out of which 30 teeth were normal (sound teeth without any associated pathologies) and 30 were periodontally involved teeth respectively. Longitudinal ground sections were prepared and observed under phase contrast microscope. Measurements were made using an image analyzer software. The total width of the cementum was divided by the distance between two incremental lines. The eruption age of the tooth was then added to this to obtain the chronologic age for each individual. The results in the present study showed that tooth cemental annulations are applicable to periodontally sound teeth as well as in periodontally diseased teeth. There was no significant difference of estimated age from the actual age in both periodontally sound and periodontally diseased teeth. Normal teeth showed a reliability value of 92% and periodontally compromised teeth showed 96% respectively. There was no substantial influence of periodontal health on the estimated age.

Conclusions

The study concludes that the use of phase contrast microscopy in conjunction with image enhancement procedures improves the accuracy of age estimation and may serve as a reliable aid in forensic identification.
The sustainability of reforesting landscapes with exotic species: a case study of eucalypts in Ethiopia
Authors: Zerga Belay , Warkineh Bikila , Teketay Demel , Woldetsadik Muluneh
Published: November 2021

Eucalypt reforestation has been a common feature of many countries, especially in the developing countries, such as Ethiopia.Farmers in Ethiopia plant large numbers of eucalypts on small areas of land and manage them to yield a variety of products, including leaves and small branches for fuelwood, and poles and posts for house building and other farm uses. Many people in Ethiopia are dependent on eucalypts as a source of fuel and house building material. The use of trees, especially eucalypts, as a living bank account, to be harvested when there is a need for cash, is widespread. This being the reality, the arguments for and against planting eucalypts in Ethiopia has been mounting from time to time, especially associated with water use, soil fertility, soil erosion, allelopathic effects, understory vegetation and plant diversity as well as wild animals. The paradox of reforestation using eucalypts results, mostly, from inappropriate species-site matching and poor management rather than the inherent biological characteristics of the species. Therefore, the debate on eucalypts under the pretext of concern for indigenous species and natural forests should shift to how both plantations established using eucalypts and indigenous species as well as natural forests can help in the enhancement of the socio-economic development and environmental conservation of countries, such as Ethiopia. Despite the claimed negative impacts of eucalypts, farmers in Ethiopia have utilized their traditional knowledge and experience in establishing and managing eucalypt stands. In addition, the available reports in Ethiopia are in favour of planting eucalypts since the authors acknowledge that: (i) the negative impacts can be minimized provided that the choice of species and site as well as the management of the stands are appropriate, (ii) the benefit derived can offset the losses that can occur from such plantations, (iii) no other species seems to replace them to bridge the ever-widening gap between demand and supply of wood and (iv) the profit derived from eucalypt plantations is considerably higher than cultivating crops. However, the choice of eucalypt species should be based on many criteria, for example maximum wood production, ecological sustainability, marketability of the planted species and usefulness of the species to the local populations. All these criteria involve not only a choice of species planted, but also a choice of plantation management methods from initial planting to final cutting of the trees. Careful selection of appropriate species and matching them with appropriate sites must be taken as prerequisite, and the right management practices should be employed.
Recommendations of the Brazilian Society of Rheumatology for the use of JAK inhibitors in the management of rheumatoid arthritis
Authors: Bonfiglioli Karina Rossi , da Mota Licia Maria Henrique , de Medeiros Ribeiro Ana Cristina , Kakehasi Adriana Maria , Laurindo Ieda Maria Magalhães , Giorgi Rina Dalva Neubarth , Duarte Angela Luzia Branco Pinto , Reis Ana Paula Monteiro Gomides , Ubirajara e Silva de Souza Mariana Peixoto Guimarães , Brenol Claiton Viegas , da Rocha Castelar Pinheiro Geraldo , de Albuquerque Cleandro Pires , de Moura Castro Charlles Heldan , Pinto Gustavo Luiz Behrens , Verztman Jose Fernando , Muniz Luciana Feitosa , Bertolo Manoel Barros , da Costa Pinto Maria Raquel , Louzada Júnior Paulo , Cruz Vitor Alves , Pereira Ivanio Alves , de Freitas Max Vitor Carioca , Cruz Bóris Afonso , Paiva Eduardo , Monticielo Odirlei , Provenza José Roberto , Xavier Ricardo Machado
Published: November 2021

Rheumatoid arthritis (RA) is a chronic and autoimmune systemic inflammatory disease that can cause irreversible joint deformities, with increased morbidity and mortality and a significant impact on the quality of life of the affected individual. The main objective of RA treatment is to achieve sustained clinical remission or low disease activity. However, up to 40% of patients do not respond to available treatments, including bDMARDs. New therapeutic targets for RA are emerging, such as Janus kinases (JAKs). These are essential for intracellular signaling (via JAK-STAT) in response to many cytokines involved in RA immunopathogenesis. JAK inhibitors (JAKi) have established themselves as a highly effective treatment, gaining increasing space in the therapeutic arsenal for the treatment of RA. The current recommendations aim to present a review of the main aspects related to the efficacy and safety of JAKis in RA patients, and to update the recommendations and treatment algorithm proposed by the Brazilian Society of Rheumatology in 2017.
On pion mass and decay constant from theory
Authors: Trinhammer Ole Lynnerup , Bohr Henrik G.
Published: November 2021

We calculate the pion mass from Goldstone modes in the Higgs mechanism related to the neutron decay. The Goldstone pion modes acquire mass by a vacuum misalignment of the Higgs ﬁeld. The size of the misalignment is controlled by the ratio between the electronic and the nucleonic energy scales. The nucleonic energy scale is involved in the neutron to proton transformation and the electronic scale is involved in the related creation of the electronic state in the course of the electroweak neutron decay. The respective scales inﬂuence the mapping of the intrinsic conﬁguration spaces used in our description. The conﬁguration spaces are the Lie groups U(3) for the nucleonic sector and U(2) for the electronic sector. These spaces are both compact and lead to periodic potentials in the Hamiltonians in coordinate space. The periodicity and strengths of these potentials control the vacuum misalignment and leads to a pion mass of 135.2(1.5) MeV with an uncertainty mainly from the ﬁne structure coupling at pionic energies. The pion decay constant 92 MeV results from comparing the fourth order self-coupling in an eﬀective pion ﬁeld theory with the corresponding fourth order term in the Higgs potential. We suggest analogies with the Goldberger-Treiman relation.
A fast insight into the nonlinear oscillation of nano-electro-mechanical resonators considering the size effect and the van der Waals force
Authors: Wang Kang-Jia
Published: November 2021

Nano/micro actuators are widely used in micro/nano-electro-mechanical systems (NEMS/MEMS) and the study on their nonlinear oscillation is of great significance. This paper begins with a wrong variational principle (Ghalambaz M., Appl. Nanosci., 6 (2016) 309) of the reduced governing partial differential equation of the resonator which is used to describe the nonlinear oscillation of nano-electro-mechanical resonators that takes into account the size effect and the van der Waals force. By using the semi-inverse method,the we establish the genuine variational principle. Then a simple method, the so-called He's frequency formulation, is applied to solve the problem, where only one step is needed to get the approximate amplitude-frequency relationship. Comparing with the existing method, we show that the proposed method is simple but effective, which is helpful for the study of the nonlinear oscillation in micro/nano-electro-mechanical systems.
The Time Machines of Eighteenth-Century Mewar
Authors: Chanchani Nachiket
Published: November 2021

The focus of this essay is a spectacular scroll in the British Museum's collections that has been neither exhibited nor published since its acquisition. Perhaps that is because several fundamental questions about it remain unanswered: Where and when was it made? Who made it, and for whom? What purpose and meaning did it have for the first people who saw it and those who subsequently came into contact with it? In this essay, I begin to address these elementary questions. I establish that this eleven-foot-long scroll was created in Mewar in western India in 1769, and that since then it has cleaved many realms. Those realms include art and devotion, text and textile, astral science and genealogy, classical epics and vernacular histories, and cyclical time and linear time. I then postulate that understanding this short scroll's ability to nimbly separate and join those realms can help us critically appreciate the forms, layouts, and functions of two other contemporaneous cloth scrolls from the same region that are considerably longer and also have received sparse scholarly attention. Ultimately, I show how micro studies of scrolls and scrolling practices can allow us to understand forms of knowledge in Mewar on the eve of British colonialism, and to participate in challenging certain perceptions of the region's past that remain inflected by James Tod's writings nearly two hundred years after their publication.
Paya Soup
Authors: Solomon Harris
Published: November 2021

This short story set in Mumbai imagines the enduring legacies of pandemic sickness and immunity. Everyday labors, pleasures, demands, and relations must be navigated across the fault lines of health, illness, and state surveillance.
Toward a Global Intellectual History of “Minority”
Authors: Bar Sadeh Roy , Houwink ten Cate Lotte
Published: November 2021

The term minority is today applied to describe beleaguered, persecuted, and exiled people whose subordination is preserved or merely “tolerated” by majoritarian politics inherent to modern states. As this introduction indicates, however, during the nineteenth and twentieth centuries minority politics became a rubric for sociopolitical emancipation, providing a framework for intellectuals in colonized Asia and Africa to question European powers' treatment of marginalized communities. Bar Sadeh and Houwink ten Cate contend that “minority” has unique value as an instrument for historical analysis that is restricted neither solely to minority-majority relations nor to debates about (political) representation. Instead, the authors propose a global intellectual history of “minority” as a concept and experience, which is explored in the essays compiled in this special section, “Minority Questions.” By examining the diverse genealogies of the concept of minority, the essays that follow provide a valuable contribution to efforts to redress historical wrongs, even as they offer a range of explanations for the enduring legacy and power of this multifaceted concept.
From Religious Eulogy to War Anthem
Authors: Baghoolizadeh Beeta
Published: November 2021

This article looks to two songs, “Layla Said” and “Mammad, You Weren't There to See,” to examine the politics of representation, race, religion, and nationalism in late twentieth-century Iran. “Layla Said,” a religious eulogy sung by Jahanbakhsh Kurdizadeh, would serve as inspiration for the most popular song of the Iran-Iraq War (1980–88) in terms of melody, rhythm, and lyrics. Kurdizadeh, a visibly Black Iranian, is not popularly remembered as the source of the eulogy, an omission that compounds many of the politics of Black representation in Iran. Through an investigation of film, aural recordings, photographs, and more, this article follows the many mutations of the eulogy-turned-anthem to identify the various ways ethnography and documentary works frame blackness in Iran. Kurdizadeh's life and marginalized legacy highlights the tacit erasure of blackness on the national stage in Iran.
Middle East Popular Politics in Gramscian Perspective
Authors: Chalcraft John
Published: November 2021

This article outlines a theoretical framework for researching popular politics in the Middle East and North Africa. It sketches a Gramscian alternative to existing approaches in materialist Marxism, cultural studies, and social movement studies. It also aims to think a Gramsci useful to historians, political scientists, sociologists, and anthropologists, beyond the common loci of Gramsci scholarship in political theory, comparative literature, and international relations. With a start point in Gramsci's philosophy of praxis, it puts forward a concept of popular politics as a mostly slow-moving, complex, and many-layered transformative activity, a form of historical protagonism comprising a variety of moments, capable of working changes on existing forms of hegemony and founding new social relations. The point is to enable researchers in Middle East studies to see and research popular politics, carry on a critique of transformative activity, and inform transformation in the present.
On Common Speech
Authors: Kollu Sravanthi
Published: November 2021

The multilingual turn in literary studies emphasizes the fairly recent emergence of a monolingual attachment to language. While this rightly calls into question the academic focus on monolingual competencies and offers a substantial area of inquiry for scholars working with the linguistically diverse regions of South Asia, Africa, and the Middle East, this essay posits that the persistence of multilinguality among historical actors from these regions does not merit a shift away from monolingualism in contemporary scholarship. This argument derives from the claims analyzed in this essay, made by South Asian writers in colonial India, about the singularity of one's own language (swabhasha) and the writers' anxieties to protect this language from vulgar speech (gramyam). Building on contemporary work on the vernacular, the essay seeks to draw renewed attention to the role of speech in language debates in Telugu, a language whose particularity has not become a metonym either for the nation (like Hindi) or for a pan–South Indian identity (like Tamil). In tracing the movement from vulgar speech to proper language in this archive, this essay reframes vernacularity as an ethical compulsion premised on the common.
Artists in Residence
Authors: Muyumba Walton
Published: November 2021

Mixing criticism and memoir, “Artists in Residence” offers a rumination on improvisation and collaboration in visual art-making and contemporary jazz performance. The author meditates on the 2017 Unite the Right rally and Ryan Kelly's award-winning photographs of the event and considers how artists offer models for resisting anti-Black racism and white supremacy through collaborative practices. The author analyzes the documentary films Looks of a Lot and RFK in the Land of Apartheid and reviews exhibitions by Roy DeCarava and Jason Moran, highlighting the points of intersection between jazz musicianship and visual artistry. Finally, the essay argues that artists like Kara Walker, William Kentridge, and Yusef Komunyakaa create works that express the pleasure and pain of Black Diasporic experience through practices such as blues idiom improvisation and collage. The author presents criticism as a mode of personal writing.
Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system
Authors: Hatoyama Yuki , Homma Yuta , Hiragi Shu , Fukuda Mitsunori
Published: November 2021

Two small GTPases, Rab1 and Rab5, are key membrane trafficking regulators that are conserved in all eukaryotes. They have recently been found to be essential for cell survival and/or growth in cultured mammalian cells, thereby precluding the establishment of Rab1-knockout (KO) and Rab5-KO cells, making it extremely difficult to assess the impact of complete Rab1 or Rab5 protein depletion on cellular functions. Here, we generated and analyzed cell lines with conditional KO (CKO) of either Rab1 (Rab1A and Rab1B) or Rab5 (Rab5A, Rab5B and Rab5C) by using the auxin-inducible protein degradation system. Rab1 CKO and Rab5 CKO led to eventual cell death from 18 h and 48 h, respectively, after auxin exposure. After acute Rab1 protein depletion, the Golgi stack and ribbon structures were completely disrupted, and endoplasmic reticulum (ER)-to-Golgi trafficking was severely inhibited. Moreover, we discovered a novel Rab1-depletion phenotype: perinuclear clustering of early endosomes and delayed transferrin recycling. In contrast, acute Rab5 protein depletion resulted in loss of early endosomes and late endosomes, but lysosomes appeared to be normal. We also observed a dramatic reduction in the intracellular signals of endocytic cargos via receptor-mediated or fluid-phase endocytosis in Rab5-depleted cells.
Structural model of human PORCN illuminates disease-associated variants and drug-binding sites
Authors: Yu Jia , Liao Pei-Ju , Xu Weijun , Jones Julie R. , Everman David B. , Flanagan-Steet Heather , Keller Thomas H. , Virshup David M.
Published: November 2021

Wnt signaling is essential for normal development and is a therapeutic target in cancer. The enzyme PORCN, or porcupine, is a membrane-bound O-acyltransferase (MBOAT) that is required for the post-translational modification of all Wnts, adding an essential mono-unsaturated palmitoleic acid to a serine on the tip of Wnt hairpin 2. Inherited mutations in PORCN cause focal dermal hypoplasia, and therapeutic inhibition of PORCN slows the growth of Wnt-dependent cancers. Based on homology to mammalian MBOAT proteins, we developed and validated a structural model of human PORCN. The model accommodates palmitoleoyl-CoA and Wnt hairpin 2 in two tunnels in the conserved catalytic core, shedding light on the catalytic mechanism. The model predicts how previously uncharacterized human variants of uncertain significance can alter PORCN function. Drugs including ETC-159, IWP-L6 and LGK-974 dock in the PORCN catalytic site, providing insights into PORCN pharmacologic inhibition. This structural model enhances our mechanistic understanding of PORCN substrate recognition and catalysis, as well as the inhibition of its enzymatic activity, and can facilitate the development of improved inhibitors and the understanding of disease-relevant PORCN mutants.

This article has an associated First Person interview with the joint first authors of the paper.
Comments about the appraisal of systematic reviews in restorative dentistry
Authors: Afrashtehfar Kelvin I.
Published: November 2021

Adequate adoption of evidence-based practice is deeply rooted in accessing methodological quality and completeness of systematic reviews and meta-analyses reporting. Nonetheless, this assumption might be flawed if the methodological quality assessment has not been properly conducted. Taking the former statement into consideration, this correspondence article encourages the improvement of future tertiary manuscripts, especially in the field of restorative dentistry. Thus, this article addresses an overview of reviews in restorative dentistry by Sarkis-Onofre et al. in the May 2019 issue of the Journal of Esthetic and Restorative Dentistry as an example of evaluating tertiary evidence for increasing the awareness of reviewers, authors, and readers.
Comparing the first and second waves of COVID-19 in a tertiary university hospital in Barcelona
Authors: Cossio Yolima , Aller Marta-Beatriz , Abadias Maria José , Domínguez Jose-Manuel , Romea Maria-Soledad , Barba Maria-Àngels , Rodríguez Maria-Isabel , Roman Antonio , Salazar Albert
Published: November 2021

Background:

Hospitals have constituted the limiting resource of the healthcare systems for the management of the COVID-19 pandemic. As the pandemic progressed, knowledge of the disease improved, and healthcare systems were expected to be more adapted to provide a more efficient response. The objective of this research was to compare the flow of COVID-19 patients in emergency rooms and hospital wards, between the pandemic's first and second waves at the University Hospital of Vall d’Hebron (Barcelona, Spain), and to compare the profiles, severity and mortality of COVID-19 patients between the two waves.

Methods:

A retrospective observational analysis of COVID-19 patients attending the hospital from February 24 to April 26, 2020 (first wave) and from July 24, 2020, to May 18, 2021 (second wave) was carried out. We analysed the data of the electronic medical records on patient demographics, comorbidity, severity, and mortality.

Results:

The daily number of COVID-19 patients entering the emergency rooms (ER) dropped by 65% during the second wave compared to the first wave. During the second wave, patients entering the ER were significantly younger (61 against 63 years old p<0.001) and less severely affected (39% against 48% with a triage level of resuscitation or emergency; p<0.001). ER mortality declined during the second wave (1% against 2%; p<0.000). The daily number of hospitalised COVID-19 patients dropped by 75% during the second wave. Those hospitalised during the second wave were more severely affected (20% against 10%; p<0.001) and were referred to the intensive care unit (ICU) more frequently (21% against 15%; p<0.001). Inpatient mortality showed no significant difference between the two waves.

Conclusions:

Changes in the flow, severity and mortality of COVID-19 patients entering this tertiary hospital during the two waves may reflect a better adaptation of the health care system and the improvement of knowledge on the disease.
An Atlas of Plant Transposable Elements
Authors: Pedro Daniel Longhi Fernandes , Amorim Tharcisio Soares , Varani Alessandro , Guyot Romain , Domingues Douglas Silva , Paschoal Alexandre Rossi
Published: November 2021

Advances in genomic sequencing have recently offered vast opportunities for biological exploration, unraveling the evolution and improving our understanding of Earth biodiversity. Due to distinct plant species characteristics in terms of genome size, ploidy and heterozygosity, transposable elements (TEs) are common characteristics of many genomes. TEs are ubiquitous and dispersed repetitive DNA sequences that frequently impact the evolution and composition of the genome, mainly due to their redundancy and rearrangements. For this study, we provided an atlas of TE data by employing an easy-to-use portal (APTE website). To our knowledge, this is the most extensive and standardized analysis of TEs in plant genomes. We evaluated 67 plant genomes assembled at chromosome scale, recovering a total of 49,802,023 TE records, representing a total of 47,992,091,043 (~47,62%) base pairs (bp) of the total genomic space. We observed that new types of TEs were identified and annotated compared to other data repositories. By establishing a standardized catalog of TE annotation on 67 genomes, new hypotheses, exploration of TE data and their influences on the genomes may allow a better understanding of their function and processes. All original code and an example of how we developed the TE annotation strategy is available on GitHub (Extended data).
Methods, models, mechanisms and metadata: Introducing the Nanotoxicology collection at F1000Research
Authors: Lynch Iseult , Nymark Penny , Doganis Philip , Gulumian Mary , Yoon Tae-Hyun , Martinez Diego S.T. , Afantitis Antreas
Published: November 2021

Nanotoxicology is a relatively new field of research concerning the study and application of nanomaterials to evaluate the potential for harmful effects in parallel with the development of applications. Nanotoxicology as a field spans materials synthesis and characterisation, assessment of fate and behaviour, exposure science, toxicology / ecotoxicology, molecular biology and toxicogenomics, epidemiology, safe and sustainable by design approaches, and chemoinformatics and nanoinformatics, thus requiring scientists to work collaboratively, often outside their core expertise area. This interdisciplinarity can lead to challenges in terms of interpretation and reporting, and calls for a platform for sharing of best-practice in nanotoxicology research. The F1000Research Nanotoxicology collection, introduced via this editorial, will provide a place to share accumulated best practice, via original research reports including no-effects studies, protocols and methods papers, software reports and living systematic reviews, which can be updated as new knowledge emerges or as the domain of applicability of the method, model or software is expanded. This editorial introduces the Nanotoxicology Collection in F1000Research. The aim of the collection is to provide an open access platform for nanotoxicology researchers, to support an improved culture of data sharing and documentation of evolving protocols, biological and computational models, software tools and datasets, that can be applied and built upon to develop predictive models and move towards in silico nanotoxicology and nanoinformatics. Submissions will be assessed for fit to the collection and subjected to the F1000Research open peer review process.
Good participatory practice for coronavirus disease 2019 (COVID-19) research: the case of a COVID-19 prevention study
Authors: Perrone Carlo , Schilling William , Callery James J. , Ashley Elizabeth A. , Chambers Mary , Chase Hannah , Dahal Piyush , Kanthawang Nipaphan , Nedsuwan Supalert , Hanboonkunupakarn Borimas , Intralawan Daranee , Karkey Abhilasha , Mayxay Mayfong , Souvong Vimalay , Tran Minh Hien , Udas Shakya Summita , Sharma Sanjib Kumar , Uranw Surendra , Vannachione Souphaphone , Woodrow Charles , White Nicholas J. , Cheah Phaik Yeong
Published: November 2021

Background: The COPCOV study (chloroquine/ hydroxychloroquine prevention of coronavirus disease), which started recruitment in April 2020, is a multi-country double-blind, randomised, placebo-controlled trial which is being conducted in healthcare facilities involved in COVID-19 case management. COPCOV aims to recruit healthcare workers and other staff employed in facilities managing people with proven or suspected COVID-19.

Methods: We conducted a series of engagement sessions, each involving a short presentation of the study, a section where attendees were asked to express if they would be interested in participating in such a study and which information they would need to change their view and an open Q&A section. Answers were transcribed and coded into themes by two independent investigators. Themes were derived from the data. The aims were to assess the feasibility of the study at the respective sites, to identify context-specific ethical issues, to understand concerns potential participants might have, to fine tune research procedures and to refine COPCOV information materials. They complemented other site-specific engagement, communication and public relation activities such as press releases and websites.

Results: From 16th March 2020 to 20th January 2021, 12 engagement sessions were conducted in Thailand, Laos, Vietnam, Nepal and the UK involving 213 attendees in total. The sessions were designed to encourage potential participants and research professionals not directly involved in the project to interact with those who planned the study and those conducting it. Many attendees were keen to join the study while others had concerns. Questions raised revolved around the social value and study rationale; safety of trial medications and risk-benefit balance; study design and commitments.

Conclusions: These sessions helped us refine information materials, identify misunderstandings about the study as well as complement site feasibility assessments. Our experience strongly supports the use of participatory practices prior to conducting clinical trials.
The genome sequence of the bishop’s mitre shieldbug, Aelia acuminata (Linnaeus, 1758)
Authors: Crowley Liam M. , Barclay Maxwell V.L. ,   University of Oxford and Wytham Woods Genome Acquisition Lab,   Natural History Museum Genome Acquisition Lab,   Darwin Tree of Life Barcoding collective,   Wellcome Sanger Institute Tree of Life programme,   Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective,   Tree of Life Core Informatics collective,   Darwin Tree of Life Consortium
Published: November 2021

We present a genome assembly from an individual male Aelia acuminata (the bishop’s mitre shieldbug; Arthropoda; Insecta; Hemiptera; Pentatomidae). The genome sequence is 1,170 megabases in span. The majority of the assembly (99.78%) is scaffolded into 8 chromosomal pseudomolecules, with the X and Y sex chromosome assembled.