Phyto-chemical analysis and protective potential of novel herbal formulation against neurotoxicity induced by Aβ1-42 peptide, inflammation and oxidative stress
Authors:   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco, Iskandar Samy ,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco, Gnansounou Martial Senankpon ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France,   Applied Chemistry Study and Research Laboratory, Abomey Calavi Polytechnic School, Abomey Calavi University, Benin, Ghazouane Amel ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France, Sauzet Christophe ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France, Robin Maxime ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France, El Jemli Meryem ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco, Slimani Lamia ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco, Jhilal Fayssal ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco, Abou Lydia ,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France, Di Giorgio Carole ,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France, Ahid Samir ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco, Dahouenon Edwige ,   Applied Chemistry Study and Research Laboratory, Abomey Calavi Polytechnic School, Abomey Calavi University, Benin, Piccerelle Philippe ,   Faculty of Pharmacy, Mohammed VI University of Health Sciences (UM6SS), Casablanca, Morocco,   Mediterranean Institute of Biodiversity and Marine and Continental Ecology (IMBE), Faculty of Pharmacy, Aix Marseille University, Marseille, France
Published: April 2022

Alzheimer's disease is a neurodegenerative disorder that causes a progressive deterioration of memory and intellectual capacities. It causes irreversible lesions in the brain and leads to a decline in cognitive functions. New non-drug approaches should, therefore, be explored to slow the progression of the disease. We previously demonstrated the biological activities of a plant composition, designated as AT000. This herbal extract contains a plant heretofore unlisted in the European Pharmacopoeia. In this study, we substituted this plant with camphor leaves and studied the antioxidant, anti-inflammatory and neuroprotective activities in vitro of this new formulation, designated as AT00X. The chemical composition of the AT00X extract using gas chromatography was also evaluated. Results showed significant antioxidant and anti-inflammatory activity with 224010 equivalents of Trolox, an increase in the resistance of control blood to free radical attack up to 54.21% by KRL assay and an anti-inflammatory ratio of 99.13. The extract significantly improves neuronal survival and decreases ROS production at 5 μg/ml. It also stimulates neurite network reconstitution at 10 µg/ml. It can, therefore, be concluded that AT00X extract is an effective therapeutic agent for cognitive deficits caused by Alzheimer's disease. Keywords: Alzheimer’s disease, medicinal plants, amyloid beta, oxidative stress, inflammation.