bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness of exercise on fall prevention in community-dwelling older adults: a 2-year randomized controlled study of 914 women
Authors: Rikkonen Toni , Sund Reijo , Koivumaa-Honkanen Heli , Sirola Joonas , Honkanen Risto , Kröger Heikki
Published: April 2023

Background

Communal exercise interventions may help prevent falls and injuries. However, pragmatic trials demonstrating the effectiveness of such strategies are sparse.

Methods

We determined whether a cost-free 12-month admission to the city’s recreational sports facilities including initial 6 months of supervised weekly gym and Tai Chi sessions decreases the number of falls and related injuries. The mean (SD) follow-up time was 22·6 (4.8) months in 2016–19. A total of 914 women from a population-based sample with a mean age of 76.5 (SD 3.3, range 71.1–84.8) years were randomized into exercise intervention (n = 457) and control (n = 457) groups. Fall information was collected through biweekly short message (SMS) queries and fall diaries. Altogether 1,380 falls were recorded for the intention-to-treat analysis, with 1,281 (92.8%) being verified by telephone.

Results

A 14.3% fall rate reduction was detected in the exercise group (Incidence rate ratio (IRR) = 0.86; CI 95% 0.77–0.95) compared with the control group. Approximately half of the falls caused moderate (n = 678, 52.8%) or severe (n = 61, 4.8%) injury. In total, 13.2% (n = 166) of falls (including 73 fractures) required medical consultation with a 38% lower fracture rate in the exercise group (IRR = 0.62; CI 95% 0.39–0.99). Overall, the greatest reduction of 41% (IRR = 0.59; CI 95% 0.36–0.99) was observed in falls with severe injury and pain.

Conclusions

A community-based approach for a 6-month exercise period combined with a 12-month free use of sports premises can reduce falls, fractures and other fall-related injuries in aging women.
Formation of phosphatidylethanol and ethylglucuronide after low to moderate alcohol consumption in volunteers with a previous three-week alcohol abstinence
Authors: Herzog Josefine , Skopp Gisela , Musshoff Frank , Hartung Benno
Published: April 2023

Aims

Phosphatidylethanol (PEth) is only formed when ethanol is present in blood. This direct alcohol marker has been widely discussed, including the minimum amount of ethanol being necessary to form as much PEth as to exceed the threshold of 20 ng/mL in previously PEth negative subjects. In order to corroborate hitherto existing results, a drinking study including 18 participants after a 3-week alcohol abstinence was performed.

Methods

They consumed a pre-calculated amount of ethanol to reach a blood alcohol concentration (BAC) of at least 0.6 g/kg. Blood was drawn before and periodically seven times after alcohol administration on day 1. Blood and urine were also collected the next morning. Dried blood spots (DBS) were prepared immediately from collected venous blood. BAC was determined by head space gas chromatography and the concentrations of both PEth (16:0/18:1, 16:0/18:2 and five additional homologues) and ethyl glucuronide (EtG) were analysed using liquid chromatography–tandem mass spectrometry.

Results

Out of 18, 5 participants had concentrations of PEth 16:0/18:1 above the threshold of 20 ng/mL, and 11 out of the 18 subjects had concentrations between 10 and 20 ng/mL. In addition, four persons had PEth 16:0/18:2 concentrations above 20 ng/mL the following morning. All test subjects tested positive for EtG in DBS (≥ 3 ng/mL) and urine (≥100 ng/mL) upon 20–21 h after alcohol administration.

Conclusion

By combining both a lower cutoff of 10 ng/mL and the homologue PEth 16:0/18:2, the sensitivity to detect a single alcohol intake after a 3-week abstinence increases to 72.2%.
Immunostick colorimetric assay for highly sensitive detection of food allergens by bio-nanocapsule-scaffolding technology
Authors: Sasamura Yuto , Yamamoto Seri , Tanabe Akiko , Kera Kota , Kuroda Shun'ichi , Nakayama Tsutomu , Iijima Masumi
Published: April 2023

The detection sensitivity of immunostick colorimetric assay has been increased by using a bio-nanocapsule as a scaffold for oriented immobilization of immunoglobulin Gs. This immunostick produced ∼82-folds stronger coloration in the detection of food allergens and reduced detection time by a factor of 5.
NAD+ enhances the activity and thermostability of S-adenosyl-L-homocysteine hydrolase from Pyrococcus horikoshii OT3
Authors: Iwasaki Ryuichi , Bito Tomohiro , Ishihara Atsushi , Watanabe Fumio , Yabuta Yukinori
Published: April 2023

S-Adenosyl-L-methionine (SAM) and S-adenosyl-L-homocysteine (SAH) are important biochemical intermediates. SAM is the major methyl donor for diverse methylation reactions in vivo. The SAM to SAH ratio serves as a marker of methylation capacity. Stable isotope-labeled SAM and SAH are used to measure this ratio with high sensitivity. SAH hydrolase (EC 3.13.2.1; SAHH), which reversibly catalyzes the conversion of adenosine and L-homocysteine to SAH, is used to produce labeled SAH. To produce labeled SAH with high efficiency, we focused on the SAHH of Pyrococcus horikoshii OT3, a thermophilic archaeon. We prepared recombinant P. horikoshii SAHH using Escherichia coli and investigated its enzymatic properties. Unexpectedly, the optimum temperature and thermostability of P. horikoshii SAHH were much lower than its optimum growth temperature. However, addition of NAD+ to the reaction mixture shifted the optimum temperature of P. horikoshii SAHH to a higher temperature, suggesting that NAD+ stabilizes the structure of the enzyme.
BindingSite-AugmentedDTA: enabling a next-generation pipeline for interpretable prediction models in drug repurposing
Authors: Yousefi Niloofar , Yazdani-Jahromi Mehdi , Tayebi Aida , Kolanthai Elayaraja , Neal Craig J , Banerjee Tanumoy , Gosai Agnivo , Balasubramanian Ganesh , Seal Sudipta , Ozmen Garibay Ozlem
Published: April 2023

While research into drug–target interaction (DTI) prediction is fairly mature, generalizability and interpretability are not always addressed in the existing works in this field. In this paper, we propose a deep learning (DL)-based framework, called BindingSite-AugmentedDTA, which improves drug–target affinity (DTA) predictions by reducing the search space of potential-binding sites of the protein, thus making the binding affinity prediction more efficient and accurate. Our BindingSite-AugmentedDTA is highly generalizable as it can be integrated with any DL-based regression model, while it significantly improves their prediction performance. Also, unlike many existing models, our model is highly interpretable due to its architecture and self-attention mechanism, which can provide a deeper understanding of its underlying prediction mechanism by mapping attention weights back to protein-binding sites. The computational results confirm that our framework can enhance the prediction performance of seven state-of-the-art DTA prediction algorithms in terms of four widely used evaluation metrics, including concordance index, mean squared error, modified squared correlation coefficient ($r^2_m$) and the area under the precision curve. We also contribute to three benchmark drug–traget interaction datasets by including additional information on 3D structure of all proteins contained in those datasets, which include the two most commonly used datasets, namely Kiba and Davis, as well as the data from IDG-DREAM drug-kinase binding prediction challenge. Furthermore, we experimentally validate the practical potential of our proposed framework through in-lab experiments. The relatively high agreement between computationally predicted and experimentally observed binding interactions supports the potential of our framework as the next-generation pipeline for prediction models in drug repurposing.
A strategy combining denoising and cryo-EM single particle analysis
Authors: Zhang Hui , Li Hongjia , Zhang Fa , Zhu Ping
Published: April 2023

In cryogenic electron microscopy (cryo-EM) single particle analysis (SPA), high-resolution three-dimensional structures of biological macromolecules are determined by iteratively aligning and averaging a large number of two-dimensional projections of molecules. Since the correlation measures are sensitive to the signal-to-noise ratio, various parameter estimation steps in SPA will be disturbed by the high-intensity noise in cryo-EM. However, denoising algorithms tend to damage high frequencies and suppress mid- and high-frequency contrast of micrographs, which exactly the precise parameter estimation relies on, therefore, limiting their application in SPA. In this study, we suggest combining a cryo-EM image processing pipeline with denoising and maximizing the signal’s contribution in various parameter estimation steps. To solve the inherent flaws of denoising algorithms, we design an algorithm named MScale to correct the amplitude distortion caused by denoising and propose a new orientation determination strategy to compensate for the high-frequency loss. In the experiments on several real datasets, the denoised particles are successfully applied in the class assignment estimation and orientation determination tasks, ultimately enhancing the quality of biomacromolecule reconstruction. The case study on classification indicates that our strategy not only improves the resolution of difficult classes (up to 5 Å) but also resolves an additional class. In the case study on orientation determination, our strategy improves the resolution of the final reconstructed density map by 0.34 Å compared with conventional strategy. The code is available at https://github.com/zhanghui186/Mscale.
Gain-of-function variants in the KDF1 gene cause hidradenitis suppurativa associated with ectodermal dysplasia by stabilizing IκB kinase α
Authors: Ziegler Alban , Ebstein Frédéric , Shamseldin Hanan , Prouteau Clément , Krüger Elke , Binamer Yousef M , Bonneau Dominique , Alkuraya Fowzan S , Martin Ludovic
Published: April 2023

Keratinocyte differentiation factor (KDF)1 has been shown to cause ectodermal dysplasia with or without hidradenitis suppurativa in a single family. KDF1 is known to regulate epidermal differentiation through its interaction with IκB kinase (IKK)α. We report a novel de novo KDF1 variant (p.His254Tyr) in a 20-year-old male patient presenting with hidradenitis suppurativa and ectodermal dysplasia. We demonstrate that variants in KDF1 associated with hidradenitis suppurativa actually cause pathogenic gain-of-function of KDF1 through upregulation of IKKα. Ectodermal dysplasia may be present in a subset of individuals with hidradenitis suppurativa and should be investigated. Inhibition of IKKα appears to be a suitable therapeutic target for these individuals.
The Working Mechanisms Underpinning Mental Health Experts by Experience Involvement in Direct Teaching: An Abductive Conceptual Framework
Authors: Kraus Eran , Moran Galia Sharon
Published: April 2023

Expert by experience (EBE) involvement in direct teaching is a widespread pedagogical practice in social work education. Whilst the educational outcomes of EBE involvement are established, less is known about the working mechanisms through which these outcomes are obtained. By utilising an abductive approach, this research presents a conceptual framework of the working mechanisms underpinning EBE involvement in the mental health context. Thirty-nine semi-structured interviews were conducted with ten Mental Health Experts By Experience (MHEBE), ten social work educators, ten Bachelor of Social Work students and nine Master of Social Work students. Findings from thematic analysis are combined with previously published knowledge from the EBE involvement literature to construct the conceptual framework. The framework comprised three working mechanisms: (i) sharing experiential knowledge; (ii) meeting and interacting with the MHEBE; and (iii) challenging traditional roles and power/knowledge hierarchies. The relationship between mechanisms and learning outcomes, as well as the interrelationship between the mechanisms themselves are explored and discussed. The conceptual framework advances theoretical understandings of EBE involvement and can contribute to the design and implementation of educationally effective involvement.
Less is more in language production: an information-theoretic analysis of agrammatism in primary progressive aphasia
Authors: Rezaii Neguine , Ren Boyu , Quimby Megan , Hochberg Daisy , Dickerson Bradford C
Published: April 2023

Agrammatism is a disorder of language production characterized by short, simplified sentences, the omission of function words, an increased use of nouns over verbs and a higher use of heavy verbs. Despite observing these phenomena for decades, the accounts of agrammatism have not converged. Here, we propose and test the hypothesis that the lexical profile of agrammatism results from a process that opts for words with a lower frequency of occurrence to increase lexical information. Furthermore, we hypothesize that this process is a compensatory response to patients’ core deficit in producing long, complex sentences. In this cross-sectional study, we analysed speech samples of patients with primary progressive aphasia (n = 100) and healthy speakers (n = 65) as they described a picture. The patient cohort included 34 individuals with the non-fluent variant, 41 with the logopenic variant and 25 with the semantic variant of primary progressive aphasia. We first analysed a large corpus of spoken language and found that the word types preferred by patients with agrammatism tend to have lower frequencies of occurrence than less preferred words. We then conducted a computational simulation to examine the impact of word frequency on lexical information as measured by entropy. We found that strings of words that exclude highly frequent words have a more uniform word distribution, thereby increasing lexical entropy. To test whether the lexical profile of agrammatism results from their inability to produce long sentences, we asked healthy speakers to produce short sentences during the picture description task. We found that, under this constrained condition, a similar lexical profile of agrammatism emerged in the short sentences of healthy individuals, including fewer function words, more nouns than verbs and more heavy verbs than light verbs. This lexical profile of short sentences resulted in their lower average word frequency than unconstrained sentences. We extended this finding by showing that, in general, shorter sentences get packaged with lower-frequency words as a basic property of efficient language production, evident in the language of healthy speakers and all primary progressive aphasia variants.
Traction alopecia: assessing the presentation, management and outcomes in a diverse urban population
Authors: Balazic Eliza , Hawkins Kelly , Choi Janet , Konisky Hailey , Chen Anna , Kobets Kseniya
Published: April 2023

Traction alopecia (TA) is a type of hair loss caused by repetitive tension placed on the hair follicle. An institutional review board-approved retrospective study was conducted at a single institution located in the Bronx, New York. The review identified 216 unique patients with TA and collected information on demographics, patient presentation, history, physical exam, treatment, follow-up and disease improvement. Almost all patients identified as female (98.6%), and most were Black or African American (72.7%). Mean (SD) age was 41.3 (17.1) years (median 40 years; range 1–88). Patients reported hair loss for a mean duration of 35 (51.1) months (median 18 months; range 1–264) prior to presentation. Most patients experienced asymptomatic hair loss. Around half (49.1%) of the patients attended a follow-up, with 42.5% of these patients noting improvement in hair loss or symptoms across all visits. Duration of hair loss was not associated with improvement in hair loss at follow-up visit (P = 0.23).
Analysis and Construction of Zero-Knowledge Proofs for the MinRank Problem
Authors: Song Yongcheng , Zhang Jiang , Huang Xinyi , Wu Wei , Chen Haixia
Published: April 2023

The MinRank problem is an NP-complete problem that is prevalent in multivariate cryptography and its goal is to find a non-zero linear combination of given a series of matrices over a ring such that the obtained matrix has a small rank. At Asiacrypt 2001, two Zero-Knowledge Proofs of Knowledge (ZKPoK) for the MinRank problem are proposed, and we call them MRZK and MRZK$^{\dagger }$, respectively. The latter is an improved version of the proof size of the former. However, the efficiency of MRZK$^{\dagger }$ has been open and not analyzed. While the MRZK protocol is secure, it must be repeated many times due to the soundness error $2/3$, which leads to the large proof size. For 128-bit security, the MRZK protocol is executed at least 219 iterations and the proof size is about 32 KB. In this paper, we first show that the efficiency of MRZK$^{\dagger }$ is impractical due to unreasonable parameter size. However, when the parameter size is tuned and the efficiency is improved, an imposter can be efficiently constructed. Then, to alleviate the large proof size of MRZK, inspired by the technique designing ZKPoK (Eurocrypt 2020), we propose a sigma protocol with helper to prove the solution to the MinRank problem. Finally, we transform the sigma protocol with helper into a standard ZKPoK (MRZK$^{\sharp }$) by removing the helper. The MRZK$^{\sharp }$ protocol can achieve any small soundness error and enjoy the proof size of about 15 KB (53% improvement over MRZK).
Does pre-spawning catch and release angling affect offspring telomere dynamics in Atlantic salmon?
Authors: Duncan Eleanor , Papatheodoulou Magdalene , Metcalfe Neil B , McLennan Darryl
Published: April 2023

The practice of ‘catch and release’ (C&R) angling confers a balance between animal welfare, conservation efforts and preserving the socio-economic interests of recreational angling. However, C&R angling can still cause exhaustion and physical injury, and often exposes the captured fish to the stress of air exposure. Therefore, the true conservation success of C&R angling depends on whether the angled individuals then survive to reproduction and whether there are any persisting effects on subsequent generations. Here we tested the hypothesis that the stress of C&R angling is then passed on to offspring. We experimentally manipulated the C&R experience of wild adult salmon prior to the spawning season. These parental fish either underwent a C&R simulation (which involved exercise with/without air exposure) or were left as control individuals. We then measured the telomere length of the arising offspring (at the larval stage of development) since previous studies have linked a shorter telomere length with reduced fitness/longevity and the rate of telomere loss is thought to be influenced by stress. Family-level telomere length was positively related to rate of growth. However, the telomere lengths of the salmon offspring were unrelated to the C&R experience of their parents. This may be due to there being no intergenerational effect of parental stress exposure on offspring telomeres, or to any potential effects being buffered by the significant telomere elongation mechanisms that are thought to occur during the embryonic and larval stages of development. While this may suggest that C&R angling has a minimal intergenerational effect on offspring fitness, there have been numerous other reports of negative C&R effects, therefore we should still be aiming to mitigate and refine such practices, in order to minimize their impacts on fish populations.
Development of novel layered polyglycolic acid sheet for regeneration of critical-size defect in rat trachea
Authors: Murata Yoshitake , Yutaka Yojiro , Hirata Rieko , Hidaka Yu , Hamaji Masatsugu , Yoshizawa Akihiro , Kishimoto Yo , Omori Koichi , Date Hiroshi
Published: April 2023

OBJECTIVES

Polyglycolic acid (PGA) sheets are difficult to adapt to the central airway because of poor durability against high air pressure. Therefore, we developed a novel layered PGA material to cover the central airway and examined its morphologic traits and functional performance as a potential tracheal replacement.

METHODS

A critical-size defect in rat cervical tracheas was covered with the material. Morphologic changes were bronchoscopically and pathologically evaluated. Functional performance was evaluated by regenerated ciliary area, ciliary beat frequency and ciliary transport function determined by measuring the moving distance of microspheres dropped onto the trachea (µm/s). The evaluation time points were 2 weeks, 1 month, 2 months and 6 months after surgery (n = 5, respectively).

RESULTS

Forty rats underwent implantation, and all survived. Histological examination confirmed ciliated epithelization on the luminal surface after 2 weeks. Neovascularization was observed after 1 month, tracheal glands after 2 months and chondrocyte regeneration after 6 months. Although the material was gradually replaced by self-organization, tracheomalacia was not bronchoscopically observed at any time point. The area of regenerated cilia significantly increased between 2 weeks and 1 month (12.0% vs 30.0%; P = 0.0216). The median ciliary beat frequency significantly improved between 2 weeks and 6 months (7.12 vs 10.04 Hz; P = 0.0122). The median ciliary transport function was significantly improved between 2 weeks and 2 months (5.16 vs 13.49 µm/s; P = 0.0216).

CONCLUSIONS

The novel PGA material showed excellent biocompatibility and tracheal regeneration both morphologically and functionally 6 months after tracheal implantation.
The impact of religious involvement on trust, volunteering, and perceived cooperativeness: evidence from two British panels
Authors: Aksoy Ozan , Wiertz Dingeman
Published: April 2023

Does religious involvement make people more trusting and prosocial? Considering conflicting theories and mixed prior evidence, we subject this question to a stringent test using large-scale, representative data from the British Household Panel Survey (1991–2009, N ≈ 26,000) and the UK Household Longitudinal Study (2009–2021, N ≈ 80,000). We employ cross-lagged panel models with individual fixed effects to account for time-invariant confounders and reverse causality—two issues that have haunted earlier research. We find that frequency of religious service attendance on average has a positive impact on generalized trust, volunteering, and perceived cooperativeness. Other indicators of religious involvement have weaker effects. We also find variation across religious traditions: the effects of religious attendance are mostly positive for Anglicans and other Protestants, but weaker and mostly statistically insignificant for Catholics, Hindus, and the unaffiliated, and even negative for Muslims when the outcome is perceived cooperativeness. Our findings are robust to alternative model set-ups and hold up after accounting for neighbourhood religious composition, respondent and interviewer ethnicity, and other potential moderators and confounders. Altogether, our study shows that religious involvement can foster prosocial behaviours and attitudes, although in our study this effect is largely restricted to religious service attendance and majority religions.
Pet ownership during the first 5 years after breast cancer diagnosis in the NEON-BC cohort
Authors: Lopes-Conceição Luisa , Peleteiro Bárbara , Araújo Natália , Dias Teresa , Fontes Filipa , Pereira Susana , Lunet Nuno
Published: April 2023

Background

Although human–animal interactions (HAI) have been associated with health benefits, they have not been extensively studied among cancer patients nor which factors may influence HAI during cancer survivorship. Therefore, this study aims to describe pet ownership in a breast cancer cohort within 5 years post-diagnosis and to identify associated factors.

Methods

Four hundred sixty-six patients from the NEON-BC cohort were evaluated. Four groups of pet ownership over the 5 years were defined: ‘never had’, ‘stopped having’, ‘started having’ and ‘always had’. Multinomial logistic regression was used to quantify the association between the patient characteristics and the groups defined (reference: ‘never had’).

Results

51.7% of patients had pets at diagnosis, which increased to 58.4% at 5 years; dogs and cats were the most common. Women presenting depressive symptoms and poor quality of life were more likely to stop having pets. Older and unpartnered women were less likely to start having pets. Those retired, living outside Porto, having diabetes or having owned animals during adulthood were more likely to start having pets. Women with higher education and unpartnered were less likely to always have pets. Those living in larger households, with other adults or having animals throughout life, were more likely to always have pets. Obese women had lower odds of stopping having dogs/cats. Women submitted to neoadjuvant chemotherapy and longer chemotherapy treatments were more likely to stop having dogs/cats.

Conclusions

Pet ownership changed over the 5 years and is influenced by sociodemographic, clinical and treatment characteristics, patient-reported outcomes and past pet ownership, reflecting the importance of HAI during cancer survivorship.
The effect of sample attrition in the EU Statistics on Income and Living Conditions on the estimates of Eurostat’s Healthy Life Years
Authors: Muszyńska-Spielauer Magdalena M , Spielauer Martin
Published: April 2023

Eurostat’s official Healthy Life Years (HLY) estimates are based on European Union Statistics on Income and Living Conditions (EU-SILC) cross-sectional data. As EU-SILC has a rotational sample design, the largest part of the samples are longitudinal, health-related attrition constituting a potential source of bias of these estimates. Bland-Altman plots assessing the agreement between pairs of HLY based on total and new rotational, representative samples demonstrated no significant, systematic attrition-related bias. However, the wide limits of agreement indicate considerable uncertainty, larger than accounted for in the confidence intervals of HLY estimates.
Transgressive segregation in mating traits drives hybrid speciation
Authors: Kagawa Kotaro , Takimoto Gaku , Seehausen Ole
Published: April 2023

Hybridization can rapidly generate novel genetic variation, which can promote ecological speciation by creating novel adaptive phenotypes. However, it remains unclear how hybridization, creating novel mating phenotypes (e.g., mating season, genitalia shapes, sexual displays, mate preferences), affects speciation especially when the phenotypes do not confer adaptive advantages. Here, based on individual-based evolutionary simulations, we propose that transgressive segregation of mating traits can drive incipient hybrid speciation. Simulations demonstrated that incipient hybrid speciation occurred most frequently when the hybrid population received moderate continued immigration from parental lineages causing recurrent episodes of hybridization. Recurrent hybridization constantly generated genetic variation, which promoted the rapid stochastic evolution of mating phenotypes in a hybrid population. The stochastic evolution continued until a novel mating phenotype came to dominate the hybrid population, which reproductively isolates the hybrid population from parental lineages. However, too frequent hybridization rather hindered the evolution of reproductive isolation by inflating the variation of mating phenotypes to produce phenotypes allowing mating with parental lineages. Simulations also revealed conditions for the long-term persistence of hybrid species after their incipient emergence. Our results suggest that recurrent transgressive segregation of mating phenotypes can offer a plausible explanation for hybrid speciation and radiations that involved little adaptive ecological divergence.
Sex wars: a female genital spine forces male damselflies to shorten copulation duration
Authors: Rivas-Torres Anais , Di Pietro Viviana , Cordero-Rivera Adolfo
Published: April 2023

In some species, males use weapons to harm females, increasing their short-term fitness. Here we show that females can use genital adaptations against males. Females of the damselfly Enallagma cyathigerum have a conspicuous vulvar spine on the eighth abdominal segment, which contacts with the male during copulation. We tested 3 hypotheses for its function: it (a) inflicts damage to the male during copulation; (b) facilitates endophytic oviposition; and (c) stimulates males during copulation to increase their investment. We found that males mated on average for 54 min with control females, but increased copulation to 99 min with females without spine. There was no evidence of physical harm of the spine on the male’s seminal vesicle, which shows 8- to 18-folds, exactly where the spine contacts during copulation. Females with and without spine exhibited the same egg-laying rates and showed similar fecundity and fertility. Longevity was also similar in males mated to control and spineless females. In contrast to many species where females resist male harassment by behavioral responses, the morphological adaptation observed in E. cyathigerum appears to act as a sexual weapon, allowing females to control copulation duration. We suggest that the spine has evolved because of sexual conflict over mating duration.
What Determines Levels of Mitochondrial Genetic Diversity in Birds?
Authors: Clark Alice , Koc Gizem , Eyre-Walker Ying , Eyre-Walker Adam
Published: April 2023

What determines levels of genetic diversity in mitochondrial DNA remains unresolved. We have investigated the factors that are correlated to the level of synonymous diversity of mitochondrial DNA in more than 300 bird species. We find that diversity is significantly correlated to clutch and range size, but not significantly correlated to many other variables including body mass, latitude, and longevity. The correlation between diversity and range appears to be a consequence of a correlation between range and effective population size since a measure of the effectiveness of natural selection, which is expected to be correlated to the effective population size, is also correlated to range. The slope of the relationship between diversity and range is shallow, consistent with Lewontin's paradox, and very similar to the relationship found in mammals.
Developmental expression of the Sturge–Weber syndrome-associated genetic mutation in Gnaq: a formal test of Happle's paradominant inheritance hypothesis
Authors: Wetzel-Strong Sarah E , Galeffi Francesca , Benavides Christian , Patrucco Mary , Bullock Jessica L , Gallione Carol J , Lee Han Kyu , Marchuk Douglas A
Published: April 2023

Sturge–Weber Syndrome (SWS) is a sporadic (non-inherited) syndrome characterized by capillary vascular malformations in the facial skin, leptomeninges, or the choroid. A hallmark feature is the mosaic nature of the phenotype. SWS is caused by a somatic mosaic mutation in the GNAQ gene (p.R183Q), leading to activation of the G protein, Gαq. Decades ago, Rudolf Happle hypothesized SWS as an example of “paradominant inheritance”, that is, a “lethal gene (mutation) surviving by mosaicism”. He predicted that the “presence of the mutation in the zygote will lead to death of the embryo at an early stage of development”. We have created a mouse model for SWS using gene targeting to conditionally express the GNAQ p.R183Q mutation. We have employed two different Cre-drivers to examine the phenotypic effects of expression of this mutation at different levels and stages of development. As predicted by Happle, global, ubiquitous expression of this mutation in the blastocyst stage results in 100% embryonic death. The majority of these developing embryos show vascular defects consistent with the human vascular phenotype. By contrast, global but mosaic expression of the mutation enables a fraction of the embryos to survive, but those that survive to birth and beyond do not exhibit obvious vascular defects. These data validate Happle's paradominant inheritance hypothesis for SWS and suggest the requirement of a tight temporal and developmental window of mutation expression for the generation of the vascular phenotype. Furthermore, these engineered murine alleles provide the template for the development of a mouse model of SWS that acquires the somatic mutation during embryonic development, but permits the embryo to progress to live birth and beyond, so that postnatal phenotypes can also be investigated. These mice could then also be employed in pre-clinical studies of novel therapies.
Associations Between Mid- to Late-Life Body Mass Index and Chronic Disease-Free Survival: A Nationwide Twin Study
Authors: Guo Jie , Dove Abigail , Shang Ying , Marseglia Anna , Johnell Kristina , Rizzuto Debora , Xu Weili
Published: April 2023

Background

Some studies have linked late-life overweight to a reduced mortality risk compared to normal body mass index (BMI). However, the impact of late-life overweight and its combination with mid-life BMI status on healthy survival remains unclear. We aimed to investigate whether and to what extent mid- and/or late-life overweight are associated with chronic disease-free survival.

Methods

Within the Swedish Twin Registry, 11 597 chronic disease-free twins aged 60−79 years at baseline were followed up for 18 years. BMI (kg/m2) was recorded at baseline and 25−35 years before baseline (ie, midlife) and divided as underweight (<20), normal (≥20−25), overweight (≥25−30), and obese (≥30). Incident chronic diseases (cardiovascular diseases, type 2 diabetes, and cancer) and deaths were ascertained via registries. Chronic disease-free survival was defined as years lived until the occurrence of any chronic diseases or death. Data were analyzed using multistate survival analysis.

Results

Of all participants, 5 640 (48.6%) were overweight/obese at baseline. During the follow-up, 8 772 (75.6%) participants developed at least 1 chronic disease or died. Compared to normal BMI, late-life overweight and obesity were associated with 1.1 (95% CI, 0.3, 2.0) and 2.6 (1.6, 3.5) years shorter chronic disease-free survival. Compared to normal BMI through mid- to late life, consistent overweight/obesity and overweight/obesity only in mid-life led to 2.2 (1.0, 3.4) and 2.6 (0.7, 4.4) years shorter disease-free survival, respectively.

Conclusions

Late-life overweight and obesity may shorten disease-free survival. Further research is needed to determine whether preventing overweight/obesity from mid- to late life might favor longer and healthier survival.
Detecting Narcissism From Older Adults’ Daily Language Use: A Machine Learning Approach
Authors: Zhang Shiyang , Fingerman Karen L , Birditt Kira S
Published: April 2023

Objectives

Narcissism has been associated with poorer quality social connections in late life, yet less is known about how narcissism is associated with older adults’ daily social interactions. This study explored the associations between narcissism and older adults’ language use throughout the day.

Methods

Participants aged 65–89 (N = 281) wore electronically activated recorders which captured ambient sound for 30 s every 7 min across 5–6 days. Participants also completed the Narcissism Personality Inventory-16 scale. We used Linguistic Inquiry and Word Count to extract 81 linguistic features from sound snippets and applied a supervised machine learning algorithm (random forest) to evaluate the strength of links between narcissism and each linguistic feature.

Results

The random forest model showed that the top 5 linguistic categories that displayed the strongest associations with narcissism were first-person plural pronouns (e.g., we), words related to achievement (e.g., win, success), to work (e.g., hiring, office), to sex (e.g., erotic, condom), and that signal desired state (e.g., want, need).

Discussion

Narcissism may be demonstrated in everyday life via word use in conversation. More narcissistic individuals may have poorer quality social connections because their communication conveys an emphasis on self and achievement rather than affiliation or topics of interest to the other party.
The Reciprocal Relationship of Self-Rated Health and Instrumental Help From Adult Children: Evidence From Germany
Authors: Jessee Lisa
Published: April 2023

Objectives

Receiving support from adult children may promote or be harmful for older adults’ health. Poor health, however, often precedes the need for intergenerational support. To date, few studies have addressed the relationship between instrumental help (i.e., help with household chores) and older adults’ self-rated health (SRH) simultaneously, thereby accounting for potential reverse causality. Moreover, little research has accounted for omitted variable bias.

Methods

Dynamic panel models with fixed effects provide an opportunity to address these methodological issues. Drawing on four waves of the German Ageing Survey, which provide a sample of 3,914 parents aged 40–95 years, I investigate the bidirectional linkages between instrumental help from adult children and SRH.

Results

Results suggest that prior receipt of instrumental help is not a significant predictor of future reported SRH. Similarly, previous SRH does not significantly predict the likelihood of receiving instrumental help at follow-up. Instead, earlier values of SRH and instrumental help are most important for predicting future SRH and instrumental help.

Discussion

The results shed new light on the interplay between SRH and instrumental help from adult children. The study suggests that older adults’ health and support in later life are not interdependent. I discuss these findings in relation to future policies for healthy aging to focus on interventions that facilitate optimal health in the earlier stages of the life course and for adult children to provide continued support to their parents.
Apolipoprotein M supports S1P production and conservation and mediates prolonged Akt activation via S1PR1 and S1PR3
Authors: Kiyozuka Keisuke , Zhao Xian , Konishi Akimitsu , Minamishima Yoji Andrew , Obinata Hideru
Published: April 2023

Sphingosine 1-phosphate (S1P) is one of the lipid mediators involved in diverse physiological functions. S1P circulates in blood and lymph bound to carrier proteins. Three S1P carrier proteins have been reported, albumin, apolipoprotein M (ApoM) and apolipoprotein A4 (ApoA4). The carrier-bound S1P exerts its functions via specific S1P receptors (S1PR1-5) on target cells. Previous studies showed several differences in physiological functions between albumin-bound S1P and ApoM-bound S1P. However, molecular mechanisms underlying the carrier-dependent differences have not been clarified. In addition, ApoA4 is a recently identified S1P carrier protein, and its functional differences from albumin and ApoM have not been addressed. Here, we compared the three carrier proteins in the processes of S1P degradation, release from S1P-producing cells and receptor activation. ApoM retained S1P more stable than albumin and ApoA4 in the cell culture medium when compared in the equimolar amounts. ApoM facilitated theS1P release from endothelial cells most efficiently. Furthermore, ApoM-bound S1P showed a tendency to induce prolonged activation of Akt via S1PR1 and S1PR3. These results suggest that the carrier-dependent functional differences of S1P are partly ascribed to the differences in the S1P stability, S1P-releasing efficiency and signaling duration.
Granulomatous mastitis secondary to Corynebacterium requiring surgical intervention: a complicated diagnosis
Authors: Lu Charles , Marcucci Vincent , Kibbe Erica , Patel Roshani
Published: April 2023

Corynebacterium species is a Gram-positive bacillus endogenous to human integument that has previously been associated with idiopathic granulomatous mastitis. The diagnosis and treatment of this bacteria may be complicated by inability to distinguish colonization from contamination and infection. We present an uncommon case of granulomatous mastitis associated with negative wound cultures requiring surgical intervention.
Heparin Concentration in Evacuated Tubes and Its Effect on pH, Ionized Calcium, Lactate, and Potassium in Venous Blood Gas Analysis
Authors: Mahler Katherine , Kerlin Rae , Jones JoAnna , Li Jieli
Published: April 2023

Arterial blood specimens collected in evacuated tubes are unacceptable for blood gas analysis. However, evacuated tubes are routinely used for venous blood-gas analysis. The impact of the blood to heparin ratio on venous blood in evacuated tubes is unclear. Venous blood was drawn into lithium and sodium heparin evacuated tubes that were 1/3 full, ½ full, 2/3 full, and fully filled. Specimens were analyzed for pH, ionized calcium (iCa), lactate, and potassium on a blood-gas analyzer. The results for specimens filled only 1/3 full for lithium and sodium heparin tubes revealed a significant increase in pH and a significant decrease in the iCa. Underfilling the lithium and sodium heparin evacuated tubes did not significantly impact the lactate or potassium results. Venous whole-blood specimens should be filled to at least 2/3 full for accurate pH and iCa results.
Provenance visualization: Tracing people, processes, and practices through a data-driven approach to provenance
Authors: Vancisin Tomas , Clarke Loraine , Orr Mary , Hinrichs Uta
Published: April 2023

Provenance disclosure—the documentation of an artifact’s origin and how it was produced—is an important aspect to consider when working with historical records which undergo multiple transformations in preparation for and during digitization. Provenance in this context is commonly communicated through explanatory text or static diagrams. However, the methodological and curatorial decisions that have influenced the records’ data are easily overlooked, in particular when exploring the records through visualization as a result of digitization processes. We propose a data-driven approach to provenance disclosure which (1) traces provenance back to when the records were created, (2) documents and categorizes the records’ transformations (transcriptions, content modifications, changes in organization, and representational form), and (3) uses data visualization to disclose provenance in interactive ways. We reflect on how this approach can be practically applied in the context of historical record collections, and we present findings from a qualitative study we conducted to investigate the merits and limitations of provenance-driven visualization. Our findings suggest that data-driven provenance disclosure has the potential to (1) promote transparency and deeper interpretations of historical records, (2) provide rigor in researching historical document collections and underlying production processes, and (3) encourage ethical considerations by making visible labor and implicit bias that influence the production and curation of historical records.
Radio AGN selection and characterization in three Deep-Drilling Fields of the Vera C. Rubin Observatory Legacy Survey of Space and Time
Authors: Zhu Shifu , Brandt W N , Zou Fan , Luo Bin , Ni Qingling , Xue Yongquan , Yan Wei
Published: April 2023

The Australia Telescope Large Area Survey (ATLAS) and the VLA survey in the XMM-LSS/VIDEO deep field provide deep (≈15 $\mu$ Jy beam−1) and high-resolution (≈4.5–8 arcsec) radio coverage of the three XMM-SERVS fields (W-CDF-S, ELAIS-S1, and XMM-LSS). These data cover a total sky area of 11.3 deg2 and contain ≈11 000 radio components. Furthermore, about 3 deg2 of the XMM-LSS field also has deeper MIGHTEE data that achieve a median RMS of 5.6 $\mu$ Jy beam−1 and detect more than 20 000 radio sources. We analyse all these radio data and find source counterparts at other wavebands utilizing deep optical and infrared (IR) surveys. The nature of these radio sources is studied using radio-band properties (spectral slope and morphology) and the IR–radio correlation. Radio AGNs are selected and compared with those selected using other methods (e.g. X-ray). We found 1656 new AGNs that were not selected using X-ray and/or MIR methods. We constrain the FIR-to-UV SEDs of radio AGNs using cigale and investigate the dependence of radio AGN fraction upon galaxy stellar mass and star formation rate.
Exploring the impact of IMF and binary parameter stochasticity with a binary population synthesis code
Authors: Stanway Elizabeth R , Eldridge J J
Published: April 2023

Low-mass star formation regions are unlikely to fully populate their initial mass functions (IMFs), leading to a deficit of massive stars. In binary stellar populations, the full range of binary separations and mass ratios will also be underpopulated. To explore the effects of stochastic sampling in the integrated light of stellar clusters, we calculate models at a broad range of cluster masses, from 102 to 107 M⊙, using a binary stellar population synthesis code. For clusters with stellar masses less than 105 M⊙, observable quantities show substantial scatter and their mean properties reflect the expected deficit of massive stars. In common with previous work, we find that purely stochastic sampling of the IMF appears to underestimate the mass of the most massive star in known clusters. However, even with this constraint, the majority of clusters likely inject sufficient kinetic energy to clear their birth clusters of gas. For quantities that directly measure the impact of the most massive stars, such as Nion, ξion, and βUV, uncertainties due to stochastic sampling dominate over those from the IMF shape or distribution of binary parameters, while stochastic sampling has a negligible effect on the stellar continuum luminosity density.
High-fidelity reproduction of central galaxy joint distributions with neural networks
Authors: Rodrigues Natália V N , de Santi Natalí S M , Montero-Dorta Antonio D , Abramo L Raul
Published: April 2023

The relationship between galaxies and haloes is central to the description of galaxy formation and a fundamental step towards extracting precise cosmological information from galaxy maps. However, this connection involves several complex processes that are interconnected. Machine Learning methods are flexible tools that can learn complex correlations between a large number of features, but are traditionally designed as deterministic estimators. In this work, we use the IllustrisTNG300-1 simulation and apply neural networks in a binning classification scheme to predict probability distributions of central galaxy properties, namely stellar mass, colour, specific star formation rate, and radius, using as input features the halo mass, concentration, spin, age, and the overdensity on a scale of 3 h−1 Mpc. The model captures the intrinsic scatter in the relation between halo and galaxy properties, and can thus be used to quantify the uncertainties related to the stochasticity of the galaxy properties with respect to the halo properties. In particular, with our proposed method, one can define and accurately reproduce the properties of the different galaxy populations in great detail. We demonstrate the power of this tool by directly comparing traditional single-point estimators and the predicted joint probability distributions, and also by computing the power spectrum of a large number of tracers defined on the basis of the predicted colour–stellar mass diagram. We show that the neural networks reproduce clustering statistics of the individual galaxy populations with excellent precision and accuracy.
POLAR - I: linking the 21-cm signal from the epoch of reionization to galaxy formation
Authors: Ma Qing-Bo , Ghara Raghunath , Ciardi Benedetta , Iliev Ilian T , Koopmans Léon V E , Mellema Garrelt , Mondal Rajesh , Zaroubi Saleem
Published: April 2023

To self-consistently model galactic properties, reionization of the intergalactic medium, and the associated 21-cm signal, we have developed the algorithm polar by integrating the one-dimensional radiative transfer code grizzly with the semi-analytical galaxy formation code L-Galaxies 2020. Our proof-of-concept results are consistent with observations of the star formation rate history, UV luminosity function, and the CMB Thomson scattering optical depth. We then investigate how different galaxy formation models affect UV luminosity functions and 21-cm power spectra, and find that while the former are most sensitive to the parameters describing the merger of haloes, the latter have a stronger dependence on the supernovae feedback parameters, and both are affected by the escape fraction model.