bluuebunny/crossref_metadata_2025 huggingface dataset:
Knowledge, perceptions and practices on healthcare waste management and associated occupational health hazards among healthcare professionals in the Colombo District, Sri Lanka: a cross-sectional study
Authors: Udayanga Lahiru , Sahana Loganatahan , Perera Ayesha , Ranasinghe Koshila , Ranathunge Tharaka
Published: December 2023

Background

Proper Healthcare Waste (HW) management is directly influenced by the knowledge and attitudes of Healthcare Professionals (HCP). However, studies that characterize the knowledge and practices of HCP on HW management are limited in Sri Lanka. This study was conducted to characterize the knowledge, perceptions and practices of HCP on the management of HW and to determine the risk factors influencing HW related occupational health hazards in the Colombo District of Sri Lanka.

Methods

A total of 407 HCP were recruited as the study population from selected hospitals in the Colombo District. Information on socio-demographic factors, knowledge, attitudes and practices on HW management were gathered using an interviewer-administrated questionnaire. The Binary Logistic Regression (BLR) was used to determine the socio-economic risk factors associated with the occurrence of HW related health issues among the respondents.

Results

The majority of respondents were characterized with a high knowledge level (76.9%) and positive attitudes (53.8%) on HW management. Incineration (82.6%) was recognized as the most widely used HW treatment method. Personal Protective Equipment (PPE) was used at a satisfactory level (85.5%), while liquid waste treatment was limited (57.5%). The occupational designation, level of training received in HW management, professional experience, vaccination status for tetanus, degree of knowledge and attitudes on HW management were recognized as significant risk factors (p < 0.05) associated with the occurrence of HW related occupational hazards.

Conclusion

Even though, the treatment of HW was satisfactory, strengthening the existing mechanisms for monitoring of HW management, provisioning more resources and organizing training and awareness programmes on HW management for HCP are recommended.
The Archimedean Origin of Modern Positional Number Systems
Authors: Manca Vincenzo
Published: December 2023

A symbolic analysis of Archimedes’s periodical number system is developed, from which a natural link emerges with the modern positional number systems with zero. After the publication of Fibonacci’s Liber Abaci, the decimal Indo-Arabic positional system was the basis of the algorithmic and algebraic trend of modern mathematics, but even if zero plays a crucial role in algebra and mathematical analysis, zeroless positional systems show the same capability of producing efficient arithmetical algorithms based on operation tables over digits. The crucial role of digits is assessed, by considering a representation of numbers based on strings in lexicographic order. A new algorithm for the determination of decimal periods is presented by remarking on the cruciality of this topic in number theory. Periods of ordinal numbers and enumerations of recursive enumerability are shortly recalled. Concluding remarks are formulated about the deep relationship between numbers and information, which shed new light on a red line passing through the whole history of mathematics.
Aerodynamic Drag Study of the Heat Exchange Equipment with Different Fin Geometries
Authors: Korobiichuk Igor , Kostyk Sergii , Shybetskyi Vladyslav , Mogylchak Vladyslav
Published: December 2023

This article is devoted to the method of numerical modelling of aerodynamics when the air flows around fins of a special design, which is implemented in SolidWorks Flow Simulation. The study was carried out for three types of rib orientation, and the aerodynamic drag coefficients were determined for different values of the Reynolds number. It was confirmed that the drag coefficient values depend significantly on the flow regime. The lowest value of the drag coefficient is observed when the fins are oriented from a larger diameter to a smaller one. In the laminar regime (Re < 2300), the average value of CX = 1.04, in the transitional regime (2300 < Re < 10,000), CX = 0.74, and in the turbulent regime (Re > 10,000), CX = 0.22. Characteristic for this case of orientation is a significant decrease in the drag coefficient during the transition from laminar to turbulent regime; the minimum is observed at the flow speed in the range between 2 and 3 m/s.
Disparities in Breast Cancer Diagnostics: How Radiologists Can Level the Inequalities
Authors: Pesapane Filippo , Tantrige Priyan , Rotili Anna , Nicosia Luca , Penco Silvia , Bozzini Anna Carla , Raimondi Sara , Corso Giovanni , Grasso Roberto , Pravettoni Gabriella , Gandini Sara , Cassano Enrico
Published: December 2023

Access to medical imaging is pivotal in healthcare, playing a crucial role in the prevention, diagnosis, and management of diseases. However, disparities persist in this scenario, disproportionately affecting marginalized communities, racial and ethnic minorities, and individuals facing linguistic or cultural barriers. This paper critically assesses methods to mitigate these disparities, with a focus on breast cancer screening. We underscore scientific mobility as a vital tool for radiologists to advocate for healthcare policy changes: it not only enhances diversity and cultural competence within the radiology community but also fosters international cooperation and knowledge exchange among healthcare institutions. Efforts to ensure cultural competency among radiologists are discussed, including ongoing cultural education, sensitivity training, and workforce diversification. These initiatives are key to improving patient communication and reducing healthcare disparities. This paper also highlights the crucial role of policy changes and legislation in promoting equal access to essential screening services like mammography. We explore the challenges and potential of teleradiology in improving access to medical imaging in remote and underserved areas. In the era of artificial intelligence, this paper emphasizes the necessity of validating its models across a spectrum of populations to prevent bias and achieve equitable healthcare outcomes. Finally, the importance of international collaboration is illustrated, showcasing its role in sharing insights and strategies to overcome global access barriers in medical imaging. Overall, this paper offers a comprehensive overview of the challenges related to disparities in medical imaging access and proposes actionable strategies to address these challenges, aiming for equitable healthcare delivery.
Revolutionizing Cancer Treatment: Unveiling New Frontiers by Targeting the (Un)Usual Suspects
Authors: Costa Valerio , Giovannetti Elisa , Lonardo Enza
Published: December 2023

This Special Issue includes original articles and reviews on both established and innovative approaches to cancer targeting, showcased at the 29th IGB Workshop titled “Targeting the (un)usual suspects in cancer” “https://29thigbworkshop [...]
MRI-Based Assessment of Brain Tumor Hypoxia: Correlation with Histology
Authors: Arzanforoosh Fatemeh , Van der Velden Maaike , Berman Avery J. L. , Van der Voort Sebastian R. , Bos Eelke M. , Schouten Joost W. , Vincent Arnaud J. P. E. , Kros Johan M. , Smits Marion , Warnert Esther A. H.
Published: December 2023

Cerebral hypoxia significantly impacts the progression of brain tumors and their resistance to radiotherapy. This study employed streamlined quantitative blood-oxygen-level-dependent (sqBOLD) MRI to assess the oxygen extraction fraction (OEF)—a measure of how much oxygen is being extracted from vessels, with higher OEF values indicating hypoxia. Simultaneously, we utilized vessel size imaging (VSI) to evaluate microvascular dimensions and blood volume. A cohort of ten patients, divided between those with glioma and those with brain metastases, underwent a 3 Tesla MRI scan. We generated OEF, cerebral blood volume (CBV), and vessel size maps, which guided 3–4 targeted biopsies per patient. Subsequent histological analyses of these biopsies used hypoxia-inducible factor 1-alpha (HIF-1α) for hypoxia and CD31 for microvasculature assessment, followed by a correlation analysis between MRI and histological data. The results showed that while the sqBOLD model was generally applicable to brain tumors, it demonstrated discrepancies in some metastatic tumors, highlighting the need for model adjustments in these cases. The OEF, CBV, and vessel size maps provided insights into the tumor’s hypoxic condition, showing intertumoral and intratumoral heterogeneity. A significant relationship between MRI-derived measurements and histological data was only evident in the vessel size measurements (r = 0.68, p < 0.001).
Biosynthesis of Gamma-Aminobutyric Acid (GABA) by Lactiplantibacillus plantarum in Fermented Food Production
Authors: Iorizzo Massimo , Paventi Gianluca , Di Martino Catello
Published: December 2023

In recent decades, given the important role of gamma-aminobutyric acid (GABA) in human health, scientists have paid great attention to the enrichment of this chemical compound in food using various methods, including microbial fermentation. Moreover, GABA or GABA-rich products have been successfully commercialized as food additives or functional dietary supplements. Several microorganisms can produce GABA, including bacteria, fungi, and yeasts. Among GABA-producing microorganisms, lactic acid bacteria (LAB) are commonly used in the production of many fermented foods. Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) is a LAB species that has a long history of natural occurrence and safe use in a wide variety of fermented foods and beverages. Within this species, some strains possess not only good pro-technological properties but also the ability to produce various bioactive compounds, including GABA. The present review aims, after a preliminary excursus on the function and biosynthesis of GABA, to provide an overview of the current uses of microorganisms and, in particular, of L. plantarum in the production of GABA, with a detailed focus on fermented foods. The results of the studies reported in this review highlight that the selection of new probiotic strains of L. plantarum with the ability to synthesize GABA may offer concrete opportunities for the design of new functional foods.
New Developments in Asphalt Pavement: Enhancing Durability, Sustainability, and Cost-Effectiveness
Authors: Li Qiaoyi , Yang Guangqing , Si Chundi , Li Biao
Published: December 2023

Asphalt pavement is the most widely used high-grade form of pavement in road construction [...]
Diagnosis by Endoscopic Ultrasonography-Guided Sampling through the Lower Gastrointestinal Tract
Authors: Wang Jingyuan , Liu Yue , Wu Chang , Fan Jiayu , Jin Zhendong , Wang Kaixuan
Published: December 2023

Endoscopic ultrasound-guided fine-needle aspiration/biopsy (EUS-FNA/FNB) is very safe and has a high diagnostic rate for upper gastrointestinal lesions, especially pancreatic lesions, but its application in the lower gastrointestinal tract has rarely been reported. Due to the tortuous course of the colorectum, with the sigmoid colon particularly prone to perforation, most endoscopists are reluctant to perform lateral-sector endoscopic ultrasound scanning without a water-bag protection for the puncture. The ultrasonic endoscopy and flexible puncture needle techniques recently introduced into clinical practice have made ultrasound-guided puncture safer and more convenient. In addition, endoscopists have carefully tested various protective measures to improve the safety of the lower gastrointestinal puncture, substantially increasing its clinical feasibility. In this article, we review the iterations of endoscopic ultrasound equipment introduced in recent years and the many ingenious ideas proposed by endoscopists regarding lower gastrointestinal puncture.
Comparison of Multiple Models in Decentralized Target Estimation by a UAV Swarm
Authors: Lizzio Fausto Francesco , Bugaj Martin , Rostáš Ján , Primatesta Stefano
Published: December 2023

The decentralized estimation and tracking of a mobile target performed by a group of unmanned aerial vehicles (UAVs) is studied in this work. A flocking protocol is used for maintaining a collision-free formation, while a decentralized extended Kalman filter in the information form is employed to provide an estimate of the target state. In the prediction step of the filter, we adopt and compare three different models for the target motion with increasing levels of complexity, namely, a constant velocity (CV), a constant turn (CT), and a full-state (FS) model. Software-in-the-loop (SITL) simulations are conducted in ROS/Gazebo to compare the performance of the three models. The coupling between the formation and estimation tasks is evaluated since the tracking task is affected by the outcome of the estimation process.
Novel Entropy-Based Metrics for Long-Term Atrial Fibrillation Recurrence Prediction Following Surgical Ablation: Insights from Preoperative Electrocardiographic Analysis
Authors: Escribano Pilar , Ródenas Juan , García Manuel , Hornero Fernando , Gracia-Baena Juan M. , Alcaraz Raúl , Rieta José J.
Published: December 2023

Atrial fibrillation (AF) is a prevalent cardiac arrhythmia often treated concomitantly with other cardiac interventions through the Cox–Maze procedure. This highly invasive intervention is still linked to a long-term recurrence rate of approximately 35% in permanent AF patients. The aim of this study is to preoperatively predict long-term AF recurrence post-surgery through the analysis of atrial activity (AA) organization from non-invasive electrocardiographic (ECG) recordings. A dataset comprising ECGs from 53 patients with permanent AF who had undergone Cox–Maze concomitant surgery was analyzed. The AA was extracted from the lead V1 of these recordings and then characterized using novel predictors, such as the mean and standard deviation of the relative wavelet energy (RWEm and RWEs) across different scales, and an entropy-based metric that computes the stationary wavelet entropy variability (SWEnV). The individual predictors exhibited limited predictive capabilities to anticipate the outcome of the procedure, with the SWEnV yielding a classification accuracy (Acc) of 68.07%. However, the assessment of the RWEs for the seventh scale (RWEs7), which encompassed frequencies associated with the AA, stood out as the most promising individual predictor, with sensitivity (Se) and specificity (Sp) values of 80.83% and 67.09%, respectively, and an Acc of almost 75%. Diverse multivariate decision tree-based models were constructed for prediction, giving priority to simplicity in the interpretation of the forecasting methodology. In fact, the combination of the SWEnV and RWEs7 consistently outperformed the individual predictors and excelled in predicting post-surgery outcomes one year after the Cox–Maze procedure, with Se, Sp, and Acc values of approximately 80%, thus surpassing the results of previous studies based on anatomical predictors associated with atrial function or clinical data. These findings emphasize the crucial role of preoperative patient-specific ECG signal analysis in tailoring post-surgical care, enhancing clinical decision making, and improving long-term clinical outcomes.
Performance Enhancement of the Basic Solar Chimney Power Plant Integrated with an Adsorption Cooling System with Heat Recovery from the Condenser
Authors: Hassan Hassan Zohair
Published: December 2023

In a previous work, a solar chimney power plant integrated with a solid sorption cooling system for power and cold cogeneration was developed. This prior work showed that reusing the heat released from the adsorption bed enhances the system’s utilization of solar energy and increases the turbine’s output power. In the present paper, a subsequent modification to the arrangement and operation of the preceding system is introduced. The primary objective of the modification is to enhance performance and increase the plant’s capacity to effectively harness the available solar radiation. The method involves placing the condenser tubes at the solar collector entrance. Therefore, the airflow captures the condenser-released heat before it enters the collector. The modified configuration and operation of the system are discussed. A dynamic mathematical model is established to simulate the hybrid system’s operation and evaluate its parameters. The obtained results show that a 5.95% increase in output power can be achieved by recovering the heat of condensation. Furthermore, the modified system attains a 6% increase in solar-to-electricity conversion efficiency compared with the basic system. The findings suggest that the modified system, which recycles condenser heat, provides noticeable enhanced performance compared with the basic system.
Research on Efficiency of Marine Green Aquaculture in China: Regional Disparity, Driving Factors, and Dynamic Evolution
Authors: Wang Wei , Mao Wei , Zhu Jianzhen , Wu Renhong , Yang Zhenbo
Published: December 2023

It is imperative to achieve the high-quality development of fisheries and green transformation of mariculture. Based on the data of marine aquaculture fisheries in China from 2006 to 2019, this research uses the Super-SBM (Slacks-Based Measure) model to measure the efficiency of marine green aquaculture in China and analyzes the regional gap, evolution trend, and driving factors of marine green aquaculture efficiency in nine coastal provinces in China. The results are as follows: (1) The Super-SBM measurement results showed that the efficiency of marine green aquaculture in China showed a fluctuating upward trend from 2006 to 2019. The results of nuclear density estimation and Dagum Gini coefficient show that there is an obvious regional gap in the efficiency of marine green aquaculture in China, and the regional gap is increasing with time. The Dagum Gini coefficient decomposition results show that inter-regional differences and supervariable density differences were the main sources of marine green aquaculture efficiency. The results of barycentric elliptic standard deviation show that the gravity center of marine green aquaculture efficiency in China shifted to the south from 2006 to 2019, indicating that the efficiency of marine green aquaculture in southern China has had a high degree of improvement. (2) The spatial Markov chain results show that spatial proximity plays a key role in the state transfer of marine green aquaculture efficiency. The higher the efficiency of marine green aquaculture in neighboring provinces, the greater the effect on improving the efficiency of marine green aquaculture in the province. The state transfer of marine green aquaculture efficiency usually occurs between similar horizontal states, and there is less “leapfrog” transfer. (3) The results of the geographic detector show that the number of professional mariculture practitioners is the core driving factor and plays a leading role in the regional gap in the efficiency of marine green aquaculture in China. The explanatory power of the interaction of the two factors on the regional gap of marine green aquaculture efficiency is more than 90%, which is much higher than the explanatory power of a single factor. The regional gap of marine green aquaculture efficiency is the result of multiple driving factors.
Dynamics and Event-Triggered Impulsive Control of a Fractional-Order Epidemic Model with Time Delay
Authors: Liu Na , Wang Jia , Lan Qixun , Deng Wei
Published: December 2023

Due to the lack of timely protection measures against infectious diseases, or based on the particularity of the transmission of some infectious diseases and the time-varying connections between people, the transmission dynamics of infectious diseases in the information society are becoming more and more complex and changeable. A fractional-order epidemic mathematical model with network weighting and latency is proposed in this paper, and the stability near the disease-free equilibrium point and endemic equilibrium point are discussed separately. Subsequently, an event-triggered impulsive control strategy based on an infection rate threshold is put forward. By selecting the appropriate control gain, the Zeno phenomenon can be eliminated on the premise of ensuring the stability of the control error system. Finally, the theoretical results were validated numerically and some conclusions are presented. These findings contribute to future research on the limited-time event-triggered impulsive control of infectious diseases.
Unraveling the Epigenetic Tapestry: Decoding the Impact of Epigenetic Modifications in Hidradenitis Suppurativa Pathogenesis
Authors: Nardacchione Elena Maria , Tricarico Paola Maura , Moura Ronald , d’Adamo Adamo Pio , Thasneem Ayshath , Suleman Muhammad , Marzano Angelo Valerio , Crovella Sergio , Moltrasio Chiara
Published: December 2023

Hidradenitis suppurativa (HS) is a chronic autoinflammatory skin disorder, which typically occurs during puberty or early adulthood. The pathogenesis of HS is complex and multifactorial; a close interaction between hormonal, genetic, epigenetics factors, host-specific aspects, and environmental influences contributes to the susceptibility, onset, severity, and clinical course of this disease, although the exact molecular mechanisms are still being explored. Epigenetics is currently emerging as an interesting field of investigation that could potentially shed light on the molecular intricacies underlying HS, but there is much still to uncover on the subject. The aim of this work is to provide an overview of the epigenetic landscape involved in HS. Specifically, in this in-depth review we provide a comprehensive overview of DNA methylation/hydroxymethylation, histone modifications, and non-coding RNAs (such as microRNA—miRNA-132, miRNA-200c, miRNA-30a-3p, miRNA-100-5b, miRNA-155-5p, miRNA-338-5p) dysregulation in HS patients. An interesting element of epigenetic regulation in HS is that the persistent inflammatory milieu observed in HS lesional skin could be exacerbated by an altered methylation profile and histone acetylation pattern associated with key inflammatory genes. Deepening our knowledge on the subject could enable the development of targeted epigenetic therapies to potentially restore normal gene expression patterns, and subsequentially ameliorate, or even reverse, the progression of the disease. By deciphering the epigenetic code governing HS, we strive to usher in a new era of personalized and effective interventions for this enigmatic dermatological condition.
Association between Pancreatoblastoma and Familial Adenomatous Polyposis: Review of the Literature with an Additional Case
Authors: Remo Andrea , Negro Silvia , Bao Riccardo Quoc , d’Angelo Edoardo , Alaggio Rita , Crivellari Gino , Mammi Isabella , Intini Rossana , Bergamo Francesca , Fassan Matteo , Agostini Marco , Vitellaro Marco , Pucciarelli Salvatore , Urso Emanuele Damiano Luca
Published: December 2023

Background: Adult pancreatoblastoma (PBL) is a rare pancreatic malignancy, with recent evidence suggesting a possible link to familial adenomatous polyposis (FAP). This study aims to review the latest evidence and explore a possible association between adult PBL and FAP. Methods: Two independent literature reviews were conducted: (1) on PBL and FAP, and (2) on PBL in the adult population not diagnosed with FAP. Results: Out of 26 articles on PBL and FAP screened, 5 were selected for systematic review, including 1 additional case. We identified eight FAP-related PBL cases, with a median age of 40 (IQR: 34–50). Of these, seven (87%) occurred in adults. We found 65 cases of adult PBL not FAP-related; thus, 7 out of 65 cases (10.7%) of adult PBL reported in the literature are associated with a clinical diagnosis of FAP or were carriers of APC germline pathogenic variants (GPVs). Conclusion: Data suggest a non-random association between adult PBL and FAP. Further research is essential to optimise surveillance protocols and develop more effective treatment strategies.
Implementation of Brief Admission by Self-Referral in Child and Adolescent Psychiatry in Sweden: Insights from Implementers and Staff
Authors: Johansson Björn Axel , Holmström Eva , Westling Sofie , Eberhard Sophia , Rask Olof
Published: December 2023

Brief admission by self-referral, which allows patients to briefly admit themselves to a psychiatric ward, is a crisis intervention designed to reduce suicide and self-harm. This method was introduced in Sweden for adult patients in 2015, achieving high patient satisfaction and good acceptance among staff. In 2018, the method was adapted and implemented in pediatric psychiatry. The present study comprehensively describes the multifaceted strategies for implementing brief admissions, including planning, education, financing, restructuring, quality management, and policy implementation and reform. It also includes staff’s opinions of the practice of brief admissions for young people. Neither of these topics has been addressed in the existing literature. During the study period (April 2018–April 2021), 63 brief admission contracts were established. The number of new contracts increased exponentially (12.7%) per quarter (p < 0.05), and staff satisfaction with both the implementation and its benefits for unstable patients was high. Brief admission by self-referral can be successfully implemented in pediatric psychiatry and appears to be a functional crisis management method for adolescents.
Psychosocial Factors Associated with Lower Urinary Tract Symptoms One Year Postpartum
Authors: Cunningham Shayna D. , Carandang Rogie Royce , Boyd Lisa M. , Lewis Jessica B. , Ickovics Jeannette R. , Rickey Leslie M.
Published: December 2023

Pregnancy carries substantial risk for developing lower urinary tract symptoms (LUTSs), with potential lifelong impacts on bladder health. Little is known about modifiable psychosocial factors that may influence the risk of postpartum LUTSs. We examined associations between depressive symptoms, perceived stress, and postpartum LUTSs, and the moderating effects of perceived social support, using data from a cohort study of Expect With Me group prenatal care (n = 462). One year postpartum, 40.3% participants reported one or more LUTS. The most frequent LUTS was daytime frequency (22.3%), followed by urinary incontinence (19.5%), urgency (18.0%), nocturia (15.6%), and bladder pain (6.9%). Higher odds of any LUTS were associated with greater depressive symptoms (adjusted odds ratio (AOR) 1.08, 95% confidence interval (CI) 1.04–1.11) and perceived stress (AOR 1.12, 95% CI 1.04–1.19). Higher perceived social support was associated with lower odds of any LUTS (AOR 0.94, 95% CI 0.88–0.99). Perceived social support mitigated the adverse effects of depressive symptoms (interaction AOR 0.99, 95% CI 0.98–0.99) and perceived stress (interaction AOR 0.97, 95% CI 0.95–0.99) on experiencing any LUTS. Greater depressive symptoms and perceived stress may increase the likelihood of experiencing LUTSs after childbirth. Efforts to promote bladder health among postpartum patients should consider psychological factors and social support.
Annals of Education: Teaching Climate Change and Global Public Health
Authors: Rom William N.
Published: December 2023

The climate crisis is a health emergency: breaking temperature records every successive month, increasing mortality from hurricanes/cyclones resulting in >USD150 billion/year in damages, and mounting global loss of life from floods, droughts, and food insecurity. An entire course on climate change and global public health was envisioned, designed for students in public health, and delivered to Masters level students. The course content included the physical science behind global heating, heat waves, extreme weather disasters, arthropod-related diseases, allergies, air pollution epidemiology, melting ice and sea level rise, climate denialism, renewable energy and economics, social cost of carbon, and public policy. The methods included student engagement in presenting two air pollution epidemiological or experimental papers on fossil fuel air pollution. Second, they authored a mid-term paper on a specific topic in the climate crisis facing their locale, e.g., New York City. Third, they focused on a State, evaluating their climate change laws and their plans to harness renewable wind, solar, storage, nuclear, and geothermal energy. Students elsewhere covered regional entities’ approach to renewable energy. Fourth, the global impact was presented by student teams presenting a country’s nationally determined contribution to the Paris Climate Agreement. Over 200 Master’s students completed the course; the participation and feedback demonstrated markedly improved knowledge and evaluation of the course over time.
Comprehensive Analysis of PANoptosis-Related Gene Signature of Ulcerative Colitis
Authors: Wang Jun-Meng , Yang Jiao , Xia Wan-Yu , Wang Yue-Mei , Zhu Yuan-Bing , Huang Qin , Feng Tong , Xie Lu-Shuang , Li Si-Hui , Liu Shu-Qing , Yu Shu-Guang , Wu Qiao-Feng
Published: December 2023

Accumulating evidence shows that the abnormal increase in the mortality of intestinal epithelial cells (IECs) caused by apoptosis, pyroptosis, and necroptosis is closely related to the function of mucous membrane immunity and barrier function in patients with ulcerative colitis (UC). As a procedural death path that integrates the above-mentioned many deaths, the role of PANoptosis in UC has not been clarified. This study aims to explore the characterization of PANoptosis patterns and determine the potential biomarkers and therapeutic targets. We constructed a PANoptosis gene set and revealed significant activation of PANoptosis in UC patients based on multiple transcriptome profiles of intestinal mucosal biopsies from the GEO database. Comprehensive bioinformatics analysis revealed five key genes (ZBP1, AIM2, CASP1/8, IRF1) of PANoptosome with good diagnostic value and were highly correlated with an increase in pro-inflammatory immune cells and factors. In addition, we established a reliable ceRNA regulatory network of PANoptosis and predicted three potential small-molecule drugs sharing calcium channel blockers that were identified, among which flunarizine exhibited the highest correlation with a high binding affinity to the targets. Finally, we used the DSS-induced colitis model to validate our findings. This study identifies key genes of PANoptosis associated with UC development and hypothesizes that IRF1 as a TF promotes PANoptosome multicomponent expression, activates PANoptosis, and then induces IECs excessive death.
Harnessing ADAR-Mediated Site-Specific RNA Editing in Immune-Related Disease: Prediction and Therapeutic Implications
Authors: Weng Shenghui , Yang Xinyi , Yu Nannan , Wang Peng-Cheng , Xiong Sidong , Ruan Hang
Published: December 2023

ADAR (Adenosine Deaminases Acting on RNA) proteins are a group of enzymes that play a vital role in RNA editing by converting adenosine to inosine in RNAs. This process is a frequent post-transcriptional event observed in metazoan transcripts. Recent studies indicate widespread dysregulation of ADAR-mediated RNA editing across many immune-related diseases, such as human cancer. We comprehensively review ADARs’ function as pattern recognizers and their capability to contribute to mediating immune-related pathways. We also highlight the potential role of site-specific RNA editing in maintaining homeostasis and its relationship to various diseases, such as human cancers. More importantly, we summarize the latest cutting-edge computational approaches and data resources for predicting and analyzing RNA editing sites. Lastly, we cover the recent advancement in site-directed ADAR editing tool development. This review presents an up-to-date overview of ADAR-mediated RNA editing, how site-specific RNA editing could potentially impact disease pathology, and how they could be harnessed for therapeutic applications.
FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3
Authors: Xu Zhiqiang , Tian Mingyao , Tan Qihan , Hao Pengfei , Gao Zihan , Li Chang , Jin Ningyi
Published: December 2023

SARS-CoV-2 triggered the global COVID-19 pandemic, posing a severe threat to public health worldwide. The innate immune response in cells infected by SARS-CoV-2 is primarily orchestrated by type I interferon (IFN), with IFN-β exhibiting a notable inhibitory impact on SARS-CoV-2 replication. FHL2, acting as a docking site, facilitates the assembly of multiprotein complexes and regulates the transcription of diverse genes. However, the association between SARS-CoV-2 and FHL2 remains unclear. In this study, we report for the first time that SARS-CoV-2 infection in Caco2 cells results in the upregulation of FHL2 expression, while the virus’s N proteins can enhance FHL2 expression. Notably, the knockdown of FHL2 significantly amplifies SARS-CoV-2 replication in vitro. Conversely, the overexpression of FHL2 leads to a marked reduction in SARS-CoV-2 replication, with the antiviral property of FHL2 being independent of the cell or virus type. Subsequent experiments reveal that FHL2 supports IFN-β transcription by upregulating the expression and phosphorylation of IRF-3, thereby impeding SARS-CoV-2 replication in cells. These findings highlight FHL2 as a potential antiviral target for treating SARS-CoV-2 infections.
An Update on SARS-CoV-2 Clinical Trial Results—What We Can Learn for the Next Pandemic
Authors: Arman Benediktus Yohan , Brun Juliane , Hill Michelle L. , Zitzmann Nicole , von Delft Annette
Published: December 2023

The coronavirus disease 2019 (COVID-19) pandemic has claimed over 7 million lives worldwide, providing a stark reminder of the importance of pandemic preparedness. Due to the lack of approved antiviral drugs effective against coronaviruses at the start of the pandemic, the world largely relied on repurposed efforts. Here, we summarise results from randomised controlled trials to date, as well as selected in vitro data of directly acting antivirals, host-targeting antivirals, and immunomodulatory drugs. Overall, repurposing efforts evaluating directly acting antivirals targeting other viral families were largely unsuccessful, whereas several immunomodulatory drugs led to clinical improvement in hospitalised patients with severe disease. In addition, accelerated drug discovery efforts during the pandemic progressed to multiple novel directly acting antivirals with clinical efficacy, including small molecule inhibitors and monoclonal antibodies. We argue that large-scale investment is required to prepare for future pandemics; both to develop an arsenal of broad-spectrum antivirals beyond coronaviruses and build worldwide clinical trial networks that can be rapidly utilised.
Acetyl-11-Keto-β-Boswellic Acid Accelerates the Repair of Spinal Cord Injury in Rats by Resisting Neuronal Pyroptosis with Nrf2
Authors: Wang Yao , Xiong Zongliang , Zhang Qiyuan , Liu Mengmeng , Zhang Jingjing , Qi Xinyue , Jiang Xiaowen , Yu Wenhui
Published: December 2023

The primary aim of this study is to delve into the potential of Acetyl-11-keto-β-boswellic acid (AKBA) in ameliorating neuronal damage induced by acute spinal cord injury, as well as to unravel the intricate underlying mechanisms. A cohort of 40 Sprague-Dawley rats was meticulously categorized into four groups. Following a seven-day oral administration of AKBA, damaged spinal cord samples were meticulously procured for Nissl staining and electron microscopy to assess neuronal demise. Employing ELISA, immunofluorescence, Western blot (WB), and quantitative polymerase chain reaction (qPCR), the modulatory effects of AKBA within the context of spinal cord injury were comprehensively evaluated. Furthermore, employing an ex vivo extraction of spinal cord neurons, an ATP + LPS-induced pyroptotic injury model was established. The model was subsequently subjected to Nrf2 inhibition, followed by a battery of assessments involving ELISA, DCFH-DA staining, flow cytometry, immunofluorescence, and WB to decipher the effects of AKBA on the spinal cord neuron pyroptosis model. By engaging the Nrf2-ROS-NLRP3 pathway, AKBA exerted a repressive influence on the expression of the pyroptotic initiator protein Caspase-1, thereby mitigating the release of GSDMD and alleviating pyroptosis. Additionally, AKBA demonstrated the ability to attenuate the release of IL-18 and IL-1β, curbing neuronal loss and expediting the restorative processes within the context of spinal cord injury. Our study elucidates that AKBA can reduce spinal cord neuronal apoptosis, providing a basis for the development of AKBA as a clinical treatment for spinal cord injury.
New Perspectives in the Management of Chronic Hand Eczema: Lessons from Pathogenesis
Authors: Tancredi Vittorio , Buononato Dario , Caccavale Stefano , Di Brizzi Eugenia Veronica , Di Caprio Roberta , Argenziano Giuseppe , Balato Anna
Published: December 2023

Chronic hand eczema (CHE) is a common inflammatory skin condition that significantly impacts the quality of life. From work-related disabilities to social embarrassment, pain, and financial costs, the burden on society is substantial. Managing this condition presents challenges such as long-term treatment, poor patient compliance, therapy side effects, and economic feasibility. As a result, significant efforts have been made in this field in recent years. Specifically, the broader understanding of CHE pathogenesis has led to the development of new drugs, both topical and systemic. The aim of this narrative review is to summarize the current available data on hand eczema pathophysiology and explore the resulting developments in drugs for its treatment. A comprehensive search on PubMed and the other main scientific databases was conducted using keywords related to CHE and its pathogenesis. The most relevant pathways targeted by therapies include the JAK-STAT cascade, IL-4, and IL-13 axis, phosphodiesterase 4 enzyme, and chemo-attractant cytokines. In the near future, physicians will have a plethora of therapeutic alternatives. Consequently, they should be well-trained not only in how to use these alternatives but also how to combine these treatments to address the ongoing challenges related to efficacy, tolerability, and safety.
Polyacrylic-Coated Solid Nanoparticles Increase the Aquaporin Permeability to Hydrogen Peroxide
Authors: Pellavio Giorgia , Demichelis Maria Paola , Sommi Patrizia , Anselmi-Tamburini Umberto , Scotti Claudia , Laforenza Umberto
Published: December 2023

Aquaporins (AQPs) allow the diffusion of hydrogen peroxide (H2O2) and act as ROS scavenging systems, which are important for controlling the redox state of cells. Recently, cerium oxide nanoparticles were found to increase the water and H2O2 permeability by modulating AQPs. To further analyze the action of nanoparticles (NPs) on AQP, we examined the effect of the NPs presenting different core compositions (CeO2, Gd2O3, Fe3O4, and TiO2), hydrodynamic sizes, and surface functionalization. The NPs produced an increase in H2O and H2O2 permeability as a general trend. The hydrodynamic sizes of the NPs in the range of 22–100 nm did not produce any significant effect. The chemical nature of the NPs’ core did not modify the effect and its intensity. On the other hand, the NPs’ functionalized surface plays a major role in influencing both water and H2O2 permeability. The results suggest that NPs can play a significant role in controlling oxidative stress in cells and might represent an innovative approach in the treatment of a number of pathologies associated with an increased oxidative status.
Synthesis and Antibacterial Activity of Mono- and Bi-Cationic Pyridinium 1,2,4-Oxadiazoles and Triazoles
Authors: Amata Sara , Calà Cinzia , Rizzo Carla , Pibiri Ivana , Pizzo Mariangela , Buscemi Silvestre , Palumbo Piccionello Antonio
Published: December 2023

One of the main causes of mortality in humans continues to be infectious diseases. Scientists are searching for new alternatives due to the fast increase in resistance of some harmful bacteria to the frontline antibiotics. To effectively treat pathogenic infections, it is crucial to design antibiotics that can prevent the development of pathogenic resistance. For this purpose, a set of 39 quaternary pyridinium and bis-pyridinium salts with different lengths of side alkyl or fluorinated chains, heterocyclic spacers, and counter ions were tested on diverse reference bacterial ATCC (American Type Culture Collection) strains, such as S. aureus and E. coli. Subsequently, 6 out of the 39 pyridinium salts showing relevant MIC (Minimum Inhibitory Concentration) values were tested on clinically isolated, resistant strains of S. aureus, S. epidermids, S. haemolyticus, K. pneumoniae, A. baumannii, and P. aeruginosa. Additional tests have been performed to assess if the minimum concentration detected through MIC assay may limit the growth of biofilms.
Experimental Models to Study Endothelial to Mesenchymal Transition in Myocardial Fibrosis and Cardiovascular Diseases
Authors: Mimouni Mohammed , Lajoix Anne-Dominique , Desmetz Caroline
Published: December 2023

Fibrosis is a common feature of cardiovascular diseases and targets multiple organs, such as the heart and vessels. Endothelial to mesenchymal transition is a complex, vital process that occurs during embryonic formation and plays a crucial role in cardiac development. It is also a fundamental process implicated in cardiac fibrosis and repair, but also in other organs. Indeed, in numerous cardiovascular diseases, the endothelial-to-mesenchymal transition has been shown to be involved in the generation of fibroblasts that are able to produce extracellular matrix proteins such as type I collagen. This massive deposition results in tissue stiffening and organ dysfunction. To advance our understanding of this process for the development of new specific diagnostic and therapeutic strategies, it is essential to develop relevant cellular and animal models of this process. In this review, our aim was to gain an in-depth insight into existing in vitro and in vivo models of endothelial to mesenchymal transition in cardiovascular diseases with a focus on cardiac fibrosis. We discuss important parameters impacting endothelial to mesenchymal transition, and we give perspectives for the development of relevant models to decipher the underlying mechanisms and ultimately find new treatments specific to fibrosis happening in cardiovascular diseases.
LncRNA and Protein Expression Profiles Reveal Heart Adaptation to High-Altitude Hypoxia in Tibetan Sheep
Authors: He Zhaohua , Li Shaobin , Zhao Fangfang , Sun Hongxian , Hu Jiang , Wang Jiqing , Liu Xiu , Li Mingna , Zhao Zhidong , Luo Yuzhu
Published: December 2023

The Tibetan sheep has an intricate mechanism of adaptation to low oxygen levels, which is influenced by both genetic and environmental factors. The heart plays a crucial role in the adaptation of Tibetan sheep to hypoxia. In the present study, we utilized transcriptomic and proteomic technologies to comprehensively analyze and identify the long non-coding RNAs (lncRNAs), genes, proteins, pathways, and gene ontology (GO) terms associated with hypoxic adaptation in Tibetan sheep at three different altitudes (2500 m, 3500 m, and 4500 m). By integrating the differentially expressed (DE) lncRNA target genes, differentially expressed proteins (DEPs), and differentially expressed genes (DEGs), we were able to identify and characterize the mechanisms underlying hypoxic adaptation in Tibetan sheep. Through this integration, we identified 41 shared genes/proteins, and functional enrichment analyses revealed their close association with lipid metabolism, glycolysis/gluconeogenesis, and angiogenesis. Additionally, significant enrichment was observed in important pathways such as the PPAR signaling pathway, glycolysis/gluconeogenesis, the oxoacid metabolic process, and angiogenesis. Furthermore, the co-expression network of lncRNAs and mRNAs demonstrated that lncRNAs (MSTRG.4748.1, ENSOART00020025894, and ENSOART00020036371) may play a pivotal role in the adaptation of Tibetan sheep to the hypoxic conditions of the plateau. In conclusion, this study expands the existing database of lncRNAs and proteins in Tibetan sheep, and these findings may serve as a reference for the prevention of altitude sickness in humans.
Effects of Secondary Metabolites of Rice on Brown Planthopper and Its Symbionts
Authors: Deng Ziyuan , Lai Chengling , Zhang Jun , Sun Fan , Li Danting , Hao Peiying , Shentu Xuping , Pang Kun , Yu Xiaoping
Published: December 2023

The brown planthopper Nilaparvata lugens (Stål) (BPH) is a main rice pest in China and many other Asian countries. In the control of BPH, the application of insect-resistant rice has proven to be quite effective. Secondary metabolites are essential weapons in plants’ defense against phytophagous insects. Studies have found that differences in the content of secondary metabolites play a crucial role in determining whether rice exhibits resistance or susceptibility to BPH. Simultaneously, symbionts are essential to the BPH. Nevertheless, there is limited research on the impact of secondary metabolites on the symbionts within BPH. Therefore, investigating the influence of secondary metabolites on both BPH and their symbionts is significant for the control of BPH. In this experiment, newly emerged female adults of BPH were fed artificial diets containing 10 different secondary metabolites. The results indicated that methyl jasmonate had inhibitory effects on the survival rate, weight gain, and reproductive capacity of BPH. Using qPCR methods, it was discovered that the number of symbiotic fungi (Ascomycetes symbionts) within BPH significantly decreased under methyl jasmonate stress. In conclusion, this experiment has preliminarily revealed the inhibitory effects of methyl jasmonate on BPH and its symbionts, demonstrating its potential for controlling BPH.
Composition and Diversity of the Endobacteria and Ectobacteria of the Invasive Bark Beetle Hylurgus ligniperda (Fabricius) (Curculionidae: Scolytinae) in Newly Colonized Areas
Authors: Gu Ying , Ge Sixun , Li Jiale , Ren Lili , Wang Chuanzhen , Luo Youqing
Published: December 2023

Hylurgus ligniperda (Fabricius) (Curculionidae: Scolytinae) is a new invasive pest beetle in China, which colonized the Shandong province, causing devastating damage. Originating in Europe, it has spread to Oceania, Asia, North and South America. Bacterial associates have been frequently reported to play a vital role in strengthening the ecological adaptations of bark and ambrosia beetles. The environmental adaptability of H. ligniperda may be supported by their associated bacteria. Bacterial communities colonizing different body parts of insects may have different functions. However, little is known about the bacteria associated with H. ligniperda and their potential involvement in facilitating the adaptation and invasion of the beetles into new environments. In this study, we employed high-throughput sequencing technology to analyze the bacterial communities associated with male and female adults of H. ligniperda by comparing those colonizing the elytra, prothorax, and gut. Results showed that the bacterial communities of male and female adults were similar, and the elytra samples had the highest bacterial diversity and richness, followed by the gut, while the prothorax had the lowest. The dominant phyla were Proteobacteria, Firmicutes, and Actinobacteriota, while the dominant genera were Serratia, Lactococcus, Rhodococcus, unclassified Enterobacteriaceae, and Gordonia. Among these, Rhodococcus and Gordonia were the specific genera of endobacteria and ectobacteria, respectively. Differences in the distribution of associated bacteria may suggest that they have different ecological functions for H. ligniperda. The results of functional prediction showed that bacteria were enriched in terpenoid backbone biosynthesis, degradation of aromatic compounds, limonene and pinene degradation, neomycin, kanamycin and gentamicin biosynthesis, indicating that they may assist their beetles in synthesizing pheromones, degrading toxic secondary metabolites of host trees, and antagonizing pathogenic fungi. These results help us understand the interaction between H. ligniperda and bacteria and highlight possible contributions to the invasion process.