bluuebunny/crossref_metadata_2025 huggingface dataset:
Pediatric Celiac Disease Patients Show Alterations of Dendritic Cell Shape and Actin Rearrangement
Authors: Discepolo Valentina , Lania Giuliana , Ten Eikelder Maria Leonarda Gertrude , Nanayakkara Merlin , Sepe Leandra , Tufano Rossella , Troncone Riccardo , Auricchio Salvatore , Auricchio Renata , Paolella Giovanni , Barone Maria Vittoria
Published: March 2021

Celiac disease (CD) is a frequent intestinal inflammatory disease occurring in genetically susceptible individuals upon gluten ingestion. Recent studies point to a role in CD for genes involved in cell shape, adhesion and actin rearrangements, including a Rho family regulator, Rho GTPase-activating protein 31 (ARHGAP31). In this study, we investigated the morphology and actin cytoskeletons of peripheral monocyte-derived dendritic cells (DCs) from children with CD and controls when in contact with a physiological substrate, fibronectin. DCs were generated from peripheral blood monocytes of pediatric CD patients and controls. After adhesion on fibronectin, DCs showed a higher number of protrusions and a more elongated shape in CD patients compared with controls, as assessed by immunofluorescence actin staining, transmitted light staining and video time-lapse microscopy. These alterations did not depend on active intestinal inflammation associated with gluten consumption and were specific to CD, since they were not found in subjects affected by other intestinal inflammatory conditions. The elongated morphology was not a result of differences in DC activation or maturation status, and did not depend on the human leukocyte antigen (HLA)-DQ2 haplotype. Notably, we found that ARH-GAP31 mRNA levels were decreased while RhoA-GTP activity was increased in CD DCs, pointing to an impairment of the Rho pathway in CD cells. Accordingly, Rho inhibition was able to prevent the cytoskeleton rearrangements leading to the elongated morphology of celiac DCs upon adhesion on fibronectin, confirming the role of this pathway in the observed phenotype. In conclusion, adhesion on fibronectin discriminated CD from the controls’ DCs, revealing a gluten-independent CD-specific cellular phenotype related to DC shape and regulated by RhoA activity.
Perlecan in Pericellular Mechanosensory Cell-Matrix Communication, Extracellular Matrix Stabilisation and Mechanoregulation of Load-Bearing Connective Tissues
Authors: Guilak Farshid , Hayes Anthony J. , Melrose James
Published: March 2021

In this study, we review mechanoregulatory roles for perlecan in load-bearing connective tissues. Perlecan facilitates the co-acervation of tropoelastin and assembly of elastic microfibrils in translamellar cross-bridges which, together with fibrillin and elastin stabilise the extracellular matrix of the intervertebral disc annulus fibrosus. Pericellular perlecan interacts with collagen VI and XI to define and stabilize this matrix compartment which has a strategic position facilitating two-way cell-matrix communication between the cell and its wider extracellular matrix. Cues from the extracellular matrix are fed through this pericellular matrix back to the chondrocyte, allowing it to perceive and respond to subtle microenvironmental changes to regulate tissue homeostasis. Thus perlecan plays a key regulatory role in chondrocyte metabolism, and in chondrocyte differentiation. Perlecan acts as a transport proteoglycan carrying poorly soluble, lipid-modified proteins such as the Wnt or Hedgehog families facilitating the establishment of morphogen gradients that drive tissue morphogenesis. Cell surface perlecan on endothelial cells or osteocytes acts as a flow sensor in blood and the lacunar canalicular fluid providing feedback cues to smooth muscle cells regulating vascular tone and blood pressure, and the regulation of bone metabolism by osteocytes highlighting perlecan’s multifaceted roles in load-bearing connective tissues.
The Role of Hypoxia-Induced Mitogenic Factor in Organ-Specific Inflammation in the Lung and Liver: Key Concepts and Gaps in Knowledge Regarding Molecular Mechanisms of Acute or Immune-Mediated Liver Injury
Authors: Pai Sananda , Njoku Dolores B.
Published: March 2021

Hypoxia-induced mitogenic factor (HIMF), which is also known as resistin-like molecule α (RELM-α), found in inflammatory zone 1 (FIZZ1), or resistin-like alpha (retlna), is a cysteine-rich secretory protein and cytokine. HIMF has been investigated in the lung as a mediator of pulmonary fibrosis, inflammation and as a marker for alternatively activated macrophages. Although these macrophages have been found to have a role in acute liver injury and acetaminophen toxicity, few studies have investigated the role of HIMF in acute or immune-mediated liver injury. The aim of this focused review is to analyze the literature and examine the effects of HIMF and its human homolog in organ-specific inflammation in the lung and liver. We followed the guidelines set by PRISMA in constructing this review. The relevant checklist items from PRISMA were included. Items related to meta-analysis were excluded because there were no randomized controlled clinical trials. We found that HIMF was increased in most models of acute liver injury and reduced damage from acetaminophen-induced liver injury. We also found strong evidence for HIMF as a marker for alternatively activated macrophages. Our overall risk of bias assessment of all studies included revealed that 80% of manuscripts demonstrated some concerns in the randomization process. We also demonstrated some concerns (54.1%) and high risk (45.9%) of bias in the selection of the reported results. The need for randomization and reduction of bias in the reported results was similarly detected in the studies that focused on HIMF and the liver. In conclusion, we propose that HIMF could be utilized as a marker for M2 macrophages in immune-mediated liver injury. However, we also detected the need for randomized clinical trials and additional experimental and human prospective studies in order to fully comprehend the role of HIMF in acute or immune-mediated liver injury.
The Novel Benzothiazole Derivative PB11 Induces Apoptosis via the PI3K/AKT Signaling Pathway in Human Cancer Cell Lines
Authors: Kim Jinsun , Hong Sung Hee , Jeon So Hyun , Park Min Ho , Shin Cha-Gyun
Published: March 2021

Among several anti-cancer therapies, chemotherapy can be used regardless of the stage of the disease. However, development of anti-cancer agents from potential chemicals must be executed very cautiously because of several problems, such as safety, drug resistance, and continuous administration. Most chemotherapeutics selectively cause cancer cells to undergo apoptosis. In this study, we tested the effects of a novel chemical, the benzothiazole derivative N-[2-[(3,5-dimethyl-1,2-oxazol-4-yl)methylsulfanyl]-1,3-benzothiazol-6-yl]-4-oxocyclohexane-1-carboxamide (PB11) on the human cell lines U87 (glioblastoma), and HeLa (cervix cancer). It was observed that this chemical was highly cytotoxic for these cells (IC50s < 50 nM). In addition, even 40 nM PB11 induced the classical apoptotic symptoms of DNA fragmentation and nuclear condensation. The increase of caspase-3 and -9 activities also indicated an increased rate of apoptosis, which was further confirmed via Western blotting analysis of apoptosis-associated proteins. Accordingly, PB11 treatment up-regulated the cellular levels of caspase-3 and cytochrome-c, whereas it down-regulated PI3K and AKT. These results suggest that PB11 induces cytotoxicity and apoptosis in cancer cells by suppressing the PI3K/AKT signaling pathways and, thus, may serve as an anti-cancer therapeutic.
A Narrative Review on Plasminogen Activator Inhibitor-1 and Its (Patho)Physiological Role: To Target or Not to Target?
Authors: Sillen Machteld , Declerck Paul J.
Published: March 2021

Plasminogen activator inhibitor-1 (PAI-1) is the main physiological inhibitor of plasminogen activators (PAs) and is therefore an important inhibitor of the plasminogen/plasmin system. Being the fast-acting inhibitor of tissue-type PA (tPA), PAI-1 primarily attenuates fibrinolysis. Through inhibition of urokinase-type PA (uPA) and interaction with biological ligands such as vitronectin and cell-surface receptors, the function of PAI-1 extends to pericellular proteolysis, tissue remodeling and other processes including cell migration. This review aims at providing a general overview of the properties of PAI-1 and the role it plays in many biological processes and touches upon the possible use of PAI-1 inhibitors as therapeutics.
Isoform-Selective NFAT Inhibitor: Potential Usefulness and Development
Authors: Kitamura Noriko , Kaminuma Osamu
Published: March 2021

Nuclear factor of activated T cells (NFAT), which is the pharmacological target of immunosuppressants cyclosporine and tacrolimus, has been shown to play an important role not only in T cells (immune system), from which their name is derived, but also in many biological events. Therefore, functional and/or structural abnormalities of NFAT are linked to the pathogenesis of diseases in various organs. The NFAT protein family consists of five isoforms, and each isoform performs diverse functions and has unique expression patterns in the target tissues. This diversity has made it difficult to obtain ideal pharmacological output for immunosuppressants that inhibit the activity of almost all NFAT family members, causing serious and wide-ranging side effects. Moreover, it remains unclear whether isoform-selective NFAT regulation can be achieved by targeting the structural differences among NFAT isoforms and whether this strategy can lead to the development of better drugs than the existing ones. This review summarizes the role of the NFAT family members in biological events, including the development of various diseases, as well as the usefulness of and problems associated with NFAT-targeting therapies, including those dependent on current immunosuppressants. Finally, we propose a novel therapeutic strategy based on the molecular mechanisms that enable selective regulation of specific NFAT isoforms.
Molecular Aspects of Volatile Anesthetic-Induced Organ Protection and Its Potential in Kidney Transplantation
Authors: Nieuwenhuijs-Moeke Gertrude J. , Bosch Dirk J. , Leuvenink Henri G.D.
Published: March 2021

Ischemia reperfusion injury (IRI) is inevitable in kidney transplantation and negatively impacts graft and patient outcome. Reperfusion takes place in the recipient and most of the injury following ischemia and reperfusion occurs during this reperfusion phase; therefore, the intra-operative period seems an attractive window of opportunity to modulate IRI and improve short- and potentially long-term graft outcome. Commonly used volatile anesthetics such as sevoflurane and isoflurane have been shown to interfere with many of the pathophysiological processes involved in the injurious cascade of IRI. Therefore, volatile anesthetic (VA) agents might be the preferred anesthetics used during the transplantation procedure. This review highlights the molecular and cellular protective points of engagement of VA shown in in vitro studies and in vivo animal experiments, and the potential translation of these results to the clinical setting of kidney transplantation.
Adaptive Changes in the Central Control of Energy Homeostasis Occur in Response to Variations in Energy Status
Authors: Gastelum Cassandra , Perez Lynnea , Hernandez Jennifer , Le Nikki , Vahrson Isabella , Sayers Sarah , Wagner Edward J.
Published: March 2021

Energy homeostasis is regulated in coordinate fashion by the brain-gut axis, the homeostatic energy balance circuitry in the hypothalamus and the hedonic energy balance circuitry comprising the mesolimbcortical A10 dopamine pathway. Collectively, these systems convey and integrate information regarding nutrient status and the rewarding properties of ingested food, and formulate it into a behavioral response that attempts to balance fluctuations in consumption and food-seeking behavior. In this review we start with a functional overview of the homeostatic and hedonic energy balance circuitries; identifying the salient neural, hormonal and humoral components involved. We then delve into how the function of these circuits differs in males and females. Finally, we turn our attention to the ever-emerging roles of nociceptin/orphanin FQ (N/OFQ) and pituitary adenylate cyclase-activating polypeptide (PACAP)—two neuropeptides that have garnered increased recognition for their regulatory impact in energy homeostasis—to further probe how the imposed regulation of energy balance circuitry by these peptides is affected by sex and altered under positive (e.g., obesity) and negative (e.g., fasting) energy balance states. It is hoped that this work will impart a newfound appreciation for the intricate regulatory processes that govern energy homeostasis, as well as how recent insights into the N/OFQ and PACAP systems can be leveraged in the treatment of conditions ranging from obesity to anorexia.
Prediction of Functional Consequences of Missense Mutations in ANO4 Gene
Authors: Reichhart Nadine , Milenkovic Vladimir M. , Wetzel Christian H. , Strauß Olaf
Published: March 2021

The anoctamin (TMEM16) family of transmembrane protein consists of ten members in vertebrates, which act as Ca2+-dependent ion channels and/or Ca2+-dependent scramblases. ANO4 which is primarily expressed in the CNS and certain endocrine glands, has been associated with various neuronal disorders. Therefore, we focused our study on prioritizing missense mutations that are assumed to alter the structure and stability of ANO4 protein. We employed a wide array of evolution and structure based in silico prediction methods to identify potentially deleterious missense mutations in the ANO4 gene. Identified pathogenic mutations were then mapped to the modeled human ANO4 structure and the effects of missense mutations were studied on the atomic level using molecular dynamics simulations. Our data show that the G80A and A500T mutations significantly alter the stability of the mutant proteins, thus providing new perspective on the role of missense mutations in ANO4 gene. Results obtained in this study may help to identify disease associated mutations which affect ANO4 protein structure and function and might facilitate future functional characterization of ANO4.
Peri-Implantitis: A Clinical Update on Prevalence and Surgical Treatment Outcomes
Authors: Roccuzzo Andrea , Stähli Alexandra , Monje Alberto , Sculean Anton , Salvi Giovanni
Published: March 2021

Dental implants may be considered a reliable routine procedure in clinical practice for the replacement of missing teeth. Results from long-term studies indicate that implant-supported dental prostheses constitute a predictable treatment method for the management of fully and partially edentulous patients. Implants and their restorations, however, are not free from biological complications. In fact, peri-implantitis, defined as progressive bone loss associated to clinical inflammation, is not a rare finding nowadays. This constitutes a concern for clinicians and patients given the negative impact on the quality of life and the sequelae originated by peri-implantitis lesions. The purpose of this narrative review is to report on the prevalence of peri-implantitis and to overview the indications, contraindications, complexity, predictability and effectiveness of the different surgical therapeutic modalities to manage this disorder.
The Impact of Allogeneic Hematopoietic Stem Cell Transplantation on Kidney Function in Children—A Single Center Experience
Authors: Musiał Kinga , Kałwak Krzysztof , Zwolińska Danuta
Published: March 2021

Background: Knowledge about the impact of allogeneic hematopoietic stem cell transplantation (alloHSCT) on renal function in children is still limited. Objectives: The aim of the study was to evaluate kidney function in children undergoing alloHSCT, with special focus on differences between patients transplanted due to oncological and non-oncological indications. Materials and Methods: The data of 135 children undergoing alloHSCT were analyzed retrospectively. The serum creatinine and estimated glomerular filtration rate (eGFR) values were estimated before transplantation at 24 h; 1, 2, 3, 4 and 8 weeks; and 3 and 6 months after alloHSCT. Then, acute kidney injury (AKI) incidence was assessed. Results: Oncological children presented with higher eGFR values and more frequent hyperfiltration rates than non-oncological children before alloHSCT and until the 4th week after transplantation. The eGFR levels rose significantly after alloHSCT, returned to pre-transplant records after 2–3 weeks, and decreased gradually until the 6th month. AKI incidence was comparable in oncological and non-oncological patients. Conclusions: Children undergoing alloHSCT due to oncological and non-oncological reasons demonstrate the same risk of AKI, but oncological patients may be more prone to sustained renal injury. Serum creatinine and eGFR seem to be insufficient tools to assess kidney function in the early post-alloHSCT period, when hyperfiltration prevails, yet they reveal significant differences in long-term observation.
Comparing the Clinical and Laboratory Features of Remitting Seronegative Symmetrical Synovitis with Pitting Edema and Seronegative Rheumatoid Arthritis
Authors: Higashida-Konishi Misako , Izumi Keisuke , Hama Satoshi , Takei Hiroshi , Oshima Hisaji , Okano Yutaka
Published: March 2021

In seronegative arthritis with extremity edema, it is difficult to differentiate between remitting seronegative symmetrical synovitis with pitting edema syndrome (RS3PE) and seronegative rheumatoid arthritis (SNRA). We compared the clinical characteristics of RS3PE and SNRA in patients with and without malignancies. We retrospectively examined patients diagnosed with RS3PE (McCarty criteria) and SNRA at our hospital in 2007–2020. Malignancy was diagnosed within 2 years before or after RS3PE or SNRA diagnosis. Overall, 24 RS3PE and 124 SNRA patients were enrolled. The median ages were 79.5 and 68.5 years, and men comprised 54.2% and 37.1% of RS3PE and SNRA patients, respectively. RS3PE patients had higher inflammation levels (p = 0.004) and more incidences of malignancy (p = 0.034). Matching for age and sex, RS3PE patients had higher inflammation levels (p = 0.021) and more incidences of malignancy (p = 0.005). Overall, odds ratios (ORs) for malignancy were higher for older age (OR 1.06, p = 0.037), male sex (OR 4.34, p = 0.007), RS3PE patients (OR 4.83, p = 0.034), and patients with extremity edema (OR 4.83, p = 0.034). Inflammation levels and associated factors of malignancy were higher in RS3PE patients than in SNRA patients. Patients who are older, male, with extremity edema, or had RS3PE should be screened for malignancies.
The Effect of Adding Biological Factors to the Decision-Making Process for Spinal Metastasis of Non-Small Cell Lung Cancer
Authors: Kim Hyoungmin , Chang Sam Yeol , Son Jongyeon , Mok Sujung , Park Sung Cheol , Chang Bong-Soon
Published: March 2021

Molecular target therapies have markedly improved the survival of non-small cell lung cancer (NSCLC) patients, especially those with epidermal growth factor receptor (EGFR) mutations. A positive EGFR mutation is even more critical when the chronicity of spinal metastasis is considered. However, most prognostic models that estimate the life expectancy of spinal metastasis patients do not include these biological factors. We retrospectively reviewed 85 consecutive NSCLC patients who underwent palliative surgical treatment for spinal metastases to evaluate the following: (1) the prognostic value of positive EGFR mutation and the chronicity of spinal metastasis, and (2) the clinical significance of adding these two factors to an existing prognostic model, namely the New England Spinal Metastasis Score (NESMS). Among 85 patients, 38 (44.7%) were EGFR mutation-positive. Spinal metastasis presented as the initial manifestation of malignancy in 58 (68.2%) patients. The multivariate Cox proportional hazard model showed that the chronicity of spinal metastasis (hazard ratio (HR) = 1.88, p = 0.015) and EGFR mutation positivity (HR = 2.10, p = 0.002) were significantly associated with postoperative survival. The Uno’s C-index and time-dependent AUC 6 months following surgery significantly increased when these factors were added to NESMS (p = 0.004 and p = 0.022, respectively). In conclusion, biological factors provide an additional prognostic value for NSCLC patients with spinal metastasis.
Integrating Muscle Health in Predicting the Risk of Asymptomatic Vertebral Fracture in Older Adults
Authors: Lin Yu-Ching , Juan Yu-Hsiang , Chan Wing P. , Yeh Kun-Yun , Wong Alice M. K. , Sung Chen-Ming , Lin Yu-Jr , Chang Shu-Chen , Chen Fang-Ping
Published: March 2021

Background: The utility of muscle health for predicting asymptomatic vertebral fracture (VF) is uncertain. We aimed to determine the effects of muscle health on bone quantity and quality in the older adults and to integrate these factors into a predictive model for VF. Methods: We prospectively recruited participants with a body mass index <37 kg/m2. The total lean mass (TLM), appendicular skeletal muscle index, presence of sarcopenia, and bone mineral density were determined by dual-energy X-ray absorptiometry, and bone quality by the trabecular bone score (TBS). VF was diagnosed based on spine radiography. Results: A total of 414 females and 186 males were included; 257 participants had VF. Lower TLM was significantly associated with poorer bone quantity and quality in both males and females. A low TBS (OR: 11.302, p = 0.028) and sarcopenia (Odds ratio (OR): 2.820, p = 0.002) were significant predictors of VF in males, but not bone quantity. Moreover, integrating TBS and sarcopenia into the predictive model improved its performance. Conclusions: Although TLM was associated with bone quantity and quality in both sexes, sarcopenia and a low TBS were significant predictors of asymptomatic VF only in male participants.
Tracking Marine Alien Macroalgae in the Mediterranean Sea: The Contribution of Citizen Science and Remote Sensing
Authors: Mannino Anna Maria , Borfecchia Flavio , Micheli Carla
Published: March 2021

The accelerating rate of the introduction of non-indigenous species (NIS) and the magnitude of shipping traffic make the Mediterranean Sea a hotspot of biological invasions. For the effective management of NIS, early detection and intensive monitoring over time and space are essential. Here, we present an overview of possible applications of citizen science and remote sensing in monitoring alien seaweeds in the Mediterranean Sea. Citizen science activities, involving the public (e.g., tourists, fishermen, divers) in the collection of data, have great potential for monitoring NIS. The innovative methodologies, based on remote sensing techniques coupled with in situ/laboratory advanced sampling/analysis methods for tracking such species, may be useful and effective tools for easily assessing NIS distribution patterns and monitoring the space/time changes in habitats in order to support the sustainable management of the ecosystems. The reported case studies highlight how these cost-effective systems can be useful complementary tools for monitoring NIS, especially in marine protected areas, which, despite their fundamental role in the conservation of marine biodiversity, are not immune to the introduction of NIS. To ensure effective and long-lasting management strategies, collaborations between researchers, policy makers and citizens are essential.
Effects of the Marine Biotoxins Okadaic Acid and Dinophysistoxins on Fish
Authors: Corriere Mauro , Soliño Lucía , Costa Pedro Reis
Published: March 2021

Natural high proliferations of toxin-producing microorganisms in marine and freshwater environments result in dreadful consequences at the socioeconomically and environmental level due to water and seafood contamination. Monitoring programs and scientific evidence point to harmful algal blooms (HABs) increasing in frequency and intensity as a result of global climate alterations. Among marine toxins, the okadaic acid (OA) and the related dinophysistoxins (DTX) are the most frequently reported in EU waters, mainly in shellfish species. These toxins are responsible for human syndrome diarrhetic shellfish poisoning (DSP). Fish, like other marine species, are also exposed to HABs and their toxins. However, reduced attention has been given to exposure, accumulation, and effects on fish of DSP toxins, such as OA. The present review intends to summarize the current knowledge of the impact of DSP toxins and to identify the main issues needing further research. From data reviewed in this work, it is clear that exposure of fish to DSP toxins causes a range of negative effects, from behavioral and morphological alterations to death. However, there is still much to be investigated about the ecological and food safety risks related to contamination of fish with DSP toxins.
Liver Metastasectomy for Metastatic Breast Cancer Patients: A Single Institution Retrospective Analysis
Authors: Orlandi Armando , Pontolillo Letizia , Mele Caterina , Pasqualoni Mariangela , Pannunzio Sergio , Cannizzaro Maria Chiara , Cutigni Claudia , Palazzo Antonella , Garufi Giovanna , Vellone Maria , Ardito Francesco , Franceschini Gianluca , Sanchez Alejandro Martin , Cassano Alessandra , Giuliante Felice , Bria Emilio , Tortora Giampaolo
Published: March 2021

The liver represents the first metastatic site in 5–12% of metastatic breast cancer (MBC) cases. In absence of reliable evidence, liver metastasectomy (LM) could represent a possible therapeutic option for selected MBC patients (patients) in clinical practice. A retrospective analysis including MBC patients who had undergone an LM after a multidisciplinary Tumor Board discussion at the Hepatobiliary Surgery Unit of Fondazione Policlinico Universitario “Agostino Gemelli” IRCCS in Rome, between January 1994 and December 2019 was conducted. The primary endpoint was overall survival (OS) after a MBC-LM; the secondary endpoint was the disease-free interval (DFI) after surgery. Forty-nine MBC patients underwent LM, but clinical data were only available for 22 patients. After a median follow-up of 71 months, median OS and DFI were 67 months (95% CI 45–103) and 15 months (95% CI 11–46), respectively. At univariate analysis, the presence of a negative resection margin (R0) was the only factor that statistically significantly influenced OS (78 months versus 16 months; HR 0.083, p < 0.0001) and DFI (16 months versus 5 months; HR 0.17, p = 0.0058). A LM for MBC might represent a therapeutic option for selected patients. The radical nature of the surgical procedure performed in a high-flow center and after a multidisciplinary discussion appears essential for this therapeutic option.
A Holistic Perspective on Bank Performance Using Regulation, Profitability, and Risk-Taking with a View on Ownership Concentration
Authors: Rastogi Shailesh , Gupte Rajani , Meenakshi R.
Published: March 2021

There is a lack of a holistic perspective on bank performance. This study proposes a multidimensional (three-pronged) approach encompassing regulation, profitability, and nonperforming assets (NPAs) and their interactions as a measure of the performance of a bank. Moreover, the impact of equity holdings of promoters, institutional investors, and retail investors on the proposed three-pronged approach of the bank performance are also explored. Values of the concerned variables were gathered from 2016 to 2019. The dynamic panel data method was applied to empirically test the proposed model. The main findings supported the premises of the proposed approach to bank performance. Furthermore, various ownership classes provided mixed results for their impact on bank performance. Unfavorable roles of promoters and institutional investors and an indifferent role of the retail investors group were startling outcomes of the study. Successful empirical endorsement of the proposed approach for bank performance provides a fresh perspective and has varied policy- and managerial-level implications. The findings regarding various shareholder groups (ownership classes) can be a catalyst to set the policy for ownership distribution in banks, as well as shareholder protection and activism, which are conspicuously absent in India.
The Nexus between Creative Actors and Regional Development
Authors: Borseková Kamila , Vaňová Anna , Šúrová Janka , Kráľ Pavol , Turečková Kamila , Nevima Jan , Martinát Stanislav
Published: March 2021

The paper aims to deepen our understanding of the relationship and the impact of creative actors on regional development and investigates the nexus between them. The novelty of the paper lies in constructing an original criteria matrix of creative actors’ indicators which might serve as a basis for further research and policy implications. The original criteria matrix consists of two groups of indicators measuring the creative people and creative capital in the region, their mutual relationships and impact on regional development. We found that creative actors are not distributed equally across the regions in Slovakia. The strong dominance of the region with the capital city (Bratislava) was detected. We also discovered a strong direct positive relationship between representatives of the creative people and a strong direct relationship between the creative people and the emergence of small and medium enterprises (SMEs).
Planning Tool Mosaic (PTM): A Platform for Italy, a Country Without a Strategic Framework
Authors: Fiorini Lorena , Zullo Francesco , Marucci Alessandro , Di Dato Chiara , Romano Bernardino
Published: March 2021

The following work proposes the utilisation of a technical device named “Planning Tool Mosaic” (PTM), defined as a total homogeneous and standardised framework for the principles contained in municipal regulatory plans: the assignment of zoning, legends, and technical rules. The 300,000 km2-broad national territory is divided into nearly 8000 municipalities. Each of them refers to a distinct regulatory plan and then to a distinct regulation on local buildings, infrastructure, and social services. This level of planning tool is the one that has most impact on the territory. This highly fragmented scenario should be driven by upper-level regulation. Although protocols and guidelines are in force, they are almost irrelevant compared to the impact of regulation at the local level. This process is a European example of city planning mismanagement that needs to be brought to the broader attention of the European technical/scientific context. The PTM, though intervening when municipal plans are already in effect, introduces an element of social and political transparency to planning before transformative events occur. It also provides a continuous framework on the probable future of territories, thereby overcoming the current opacity with regard to public cognition of future arrangements.
An Outline of Renal Artery Stenosis Pathophysiology—A Narrative Review
Authors: Dobrek Lukasz
Published: March 2021

Renal artery stenosis (RAS) is conditioned mainly by two disturbances: fibromuscular dysplasia or atherosclerosis of the renal artery. RAS is an example of renovascular disease, with complex pathophysiology and consequences. There are multiple pathophysiological mechanisms triggered in response to significant renal artery stenosis, including disturbances within endothelin, kinin–kallikrein and sympathetic nervous systems, with angiotensin II and the renin–angiotensin-aldosterone system (RAAS) playing a central and key role in the pathogenesis of RAS. The increased oxidative stress and the release of pro-inflammatory mediators contributing to pathological tissue remodelling and renal fibrosis are also important pathogenetic elements of RAS. This review briefly summarises these pathophysiological issues, focusing on renovascular hypertension and ischemic nephropathy as major clinical manifestations of RAS. The activation of RAAS and its haemodynamic consequences is the primary and key element in the pathophysiological cascade triggered in response to renal artery stenosis. However, the pathomechanism of RAS is more complex and also includes other disturbances that ultimately contribute to the development of the diseases mentioned above. To sum up, RAS is characterised by different clinical pictures, including asymptomatic disorders diagnosed in kidney imaging, renovascular hypertension, usually characterised by severe course, and chronic ischemic nephropathy, described by pathological remodelling of kidney tissue, ultimately leading to kidney injury and chronic kidney disease.
Biosensors: A Sneak Peek into Plant Cell’s Immunity
Authors: Levak Valentina , Lukan Tjaša , Gruden Kristina , Coll Anna
Published: March 2021

Biosensors are indispensable tools to understand a plant’s immunity as its spatiotemporal dimension is key in withstanding complex plant immune signaling. The diversity of genetically encoded biosensors in plants is expanding, covering new analytes with ever higher sensitivity and robustness, but their assortment is limited in some respects, such as their use in following biotic stress response, employing more than one biosensor in the same chassis, and their implementation into crops. In this review, we focused on the available biosensors that encompass these aspects. We show that in vivo imaging of calcium and reactive oxygen species is satisfactorily covered with the available genetically encoded biosensors, while on the other hand they are still underrepresented when it comes to imaging of the main three hormonal players in the immune response: salicylic acid, ethylene and jasmonic acid. Following more than one analyte in the same chassis, upon one or more conditions, has so far been possible by using the most advanced genetically encoded biosensors in plants which allow the monitoring of calcium and the two main hormonal pathways involved in plant development, auxin and cytokinin. These kinds of biosensor are also the most evolved in crops. In the last section, we examine the challenges in the use of biosensors and demonstrate some strategies to overcome them.
Circulating Renalase as Predictor of Renal and Cardiovascular Outcomes in Pre-Dialysis CKD Patients: A 5-Year Prospective Cohort Study
Authors: Cerqueira Ana , Quelhas-Santos Janete , Ferreira Inês , Sampaio Susana , Relvas Miguel , Marques Nídia , Dias Cláudia Camila , Pestana Manuel
Published: March 2021

Chronic kidney disease (CKD) is an independent risk factor for adverse cardiovascular and cerebrovascular events (MACCEs), and mortality since the earlier stages. Therefore, it is critical to identify the link between CKD and cardiovascular risk (CVR) through early and reliable biomarkers. Acknowledging that CKD and CKD progression are associated with increased sympathetic tone, which is implicated in CVR, and that renalase metabolizes catecholamines, we aimed to evaluate the relationship between renalase serum levels (RNLS) and cardiovascular and renal outcomes. The study included 40 pre-dialysis CKD patients (19F:21M) with median age of 61 (IQ 45–66) years. At baseline, we measured RNLS as well as routine biomarkers of renal and cardiovascular risk. A prospective analysis was performed to determine whether RNLS are associated with CKD progression, MACCEs, hospitalizations and all-cause mortality. At baseline, the median level of RNLS and median estimated glomerular filtration rate (eGFR) were 63.5 (IQ 48.4–82.7) µg/mL and 47 (IQ 13–119) mL/min/1.73 m2, respectively. In univariate analysis, RNLS were strongly associated with eGFR, age and Charlson Index. Over the course of a mean follow-up of 65 (47 to 70) months, 3 (7.5%) deaths, 2 (5%) fatal MACCEs, 17 (42.5%) hospital admissions occurred, and 16 (40%) patients experienced CKD progression. In univariate analysis, RNLS were associated with CKD progression (p = 0.001), hospitalizations (p = 0.001) and all-cause mortality (p = 0.022) but not with MACCEs (p = 0.094). In adjusted analysis, RNLS predicted CKD progression and hospitalizations regardless of age, Charlson comorbidity index, cardiovascular disease, hypertension, diabetes and dyslipidemia. Our results suggest that RNLS, closely related with renal function, might have a potential role as predictor of renal outcomes, hospitalizations, and mortality in pre-dialysis CKD patients.
Valve Deadzone/Backlash Compensation for Lifting Motion Control of Hydraulic Manipulators
Authors: Li Lan , Lin Ziying , Jiang Yi , Yu Cungui , Yao Jianyong
Published: March 2021

In this paper, a novel nonlinear model and high-precision lifting motion control method of a hydraulic manipulator driven by a proportional valve are presented, with consideration of severe system nonlinearities, various uncertainties as well as valve backlash/deadzone input nonlinearities. To accomplish this mission, based on the independent valve orifice throttling process, a new comprehensive pressure-flow model is proposed to uniformly indicate both the backlash and deadzone effects on the flow characteristics. Furthermore, in the manipulator lifting dynamics, considering mechanism nonlinearity and utilizing a smooth LuGre friction model to describe the friction dynamics, a nonlinear state-space mathematical model of hydraulic manipulation system is then established. To suppress the adverse effects of severe nonlinearities and uncertainties in the system, a high precision adaptive robust control method is proposed via backstepping, in which a projection-type adaptive law in combination with a robust feedback term is conducted to attenuate various uncertainties and disturbances. Lyapunov stability analysis demonstrates that the proposed control scheme can acquire transient and steady-state close-loop stability, and the excellent tracking performance of the designed control law is verified by comparative simulation results.
The Verbal Component of Mathematical Problem Solving in Bilingual Contexts by Early Elementary Schoolers
Authors: Ester Pilar , Morales Isabel , Moraleda Álvaro , Bermejo Vicente
Published: March 2021

The main aim of the present study is to analyze the differences that may exist when students address the resolution of verbal problems in their mother tongue and in the language of instruction when these are different. We understand that knowing the type of verbal problems and their semantic structure can be helpful for students’ contextual and mathematical understanding and will allow teachers to improve instruction during the first years of elementary education in bilingual schools specialized in the area of second language acquisition as well as in CLIL (Content and Language Integrated Learning). This study shows how children, as they are acquiring a greater command of the second language, show similar effectiveness to those students who work on mathematics in their mother tongue. This transversal study was conducted on 169 bilinguals studying in international schools. The sample was made up of 80 1st grade students (39 girls, mean age of 7.1 years and 41 boys, mean age of 7.3 years); and 89 2nd grade students (38 girls, mean age 8.2 years, and 51 boys, mean age 8.2 years). The exploratory analyses let us show how 1st grade students demonstrate lower effectiveness in solving problems when they do it in a second language, compared to 2nd grade students whose effectiveness is higher in carrying them out. It is also relevant that in first graders, the largest number of errors are found in the simplest tasks as students’ effectiveness is less when they are taught in a second language, since it takes them longer to create effective resolution models. This fact will allow us to reconsider appropriate strategies and interventions when teaching mathematics in bilingual contexts.
Trigonometric Solution for the Bending Analysis of Magneto-Electro-Elastic Strain Gradient Nonlocal Nanoplates in Hygro-Thermal Environment
Authors: Tocci Monaco Giovanni , Fantuzzi Nicholas , Fabbrocino Francesco , Luciano Raimondo
Published: March 2021

Nanoplates have been extensively utilized in the recent years for applications in nanoengineering as sensors and actuators. Due to their operative nanoscale, the mechanical behavior of such structures might also be influenced by inter-atomic material interactions. For these reasons, nonlocal models are usually introduced for studying their mechanical behavior. Sensor technology of plate structures should be formulated with coupled mechanics where elastic, magnetic and electric fields interact among themselves. In addition, the effect of hygro-thermal environments are also considered since their presence might effect the nanoplate behavior. In this work a trigonometric approach is developed for investigating smart composite nanoplates using a strain gradient nonlocal procedure. Convergence of the present method is also reported in terms of displacements and electro-magnetic potentials. Results agree well with the literature and open novel applications in this field for further developments.
On the Computation of Some Interval Reliability Indicators for Semi-Markov Systems
Authors: D’Amico Guglielmo , Manca Raimondo , Petroni Filippo , Selvamuthu Dharmaraja
Published: March 2021

In this paper, we computed general interval indicators of availability and reliability for systems modelled by time non-homogeneous semi-Markov chains. First, we considered duration-dependent extensions of the Interval Reliability and then, we determined an explicit formula for the availability with a given window and containing a given point. To make the computation of the window availability, an explicit formula was derived involving duration-dependent transition probabilities and the interval reliability function. Both interval reliability and availability functions were evaluated considering the local behavior of the system through the recurrence time processes. The results are illustrated through a numerical example. They show that the considered indicators can describe the duration effects and the age of the multi-state system and be useful in real-life problems.
Synthesis of Functional Surface Layers on Stainless Steels by Laser Alloying
Authors: Duriagina Zoia , Kulyk Volodymyr , Kovbasiuk Taras , Vasyliv Bogdan , Kostryzhev Andrii
Published: March 2021

Laser alloying is an effective method to form functional surface layers (coatings) on metallic materials, particularly on stainless steels. Unique phase balance, dislocation substructure, and a possibility to obtain gradient microstructures after laser alloying slow down the surface degradation and increase the wear resistance. In this work, the optimal parameters of laser alloying and their effects on microstructure and properties were investigated for two stainless steels: ferritic AISI 420 and austenitic AISI 304. Three types of alloying plasters were used: 85Nb + 15 graphite, 85Nb + 15 liquid glass, and 15Fe + 30Ni + 20B + 10Si + 25 liquid glass (wt.%). The laser power density of 0.3 × 105 W/cm2 and beam scanning speed of 1990 mm/min were found to generate 220–320 μm thick coatings with complex microstructures. Phase balance in the coatings was studied with X-ray diffraction and magnetometric phase analyses. High microhardness (up to 16 GPa) and wear resistance were associated with the formation of martensite with some retained austenite and Nb-, Cr-, Si-, and B-rich particles in the surface layer of AISI 420 steel, and high dislocation density austenite strengthened with Ti-, Nb-, Cr-, and Si-rich particles in AISI 304 steel.
The Effect of Forging Conditions on Final Macrostructure of Slab Ingot from the 55NiCrMoV7 Tool Steel
Authors: Jonšta Petr , Kurka Vladislav , Vindyš Marek , Kander Ladislav
Published: March 2021

The paper presents numerical modelling and an operational experiment to forge a slab ingot P40N from 55NiCrMoV7 tool steel and the procedure for the optimization of its production. The aim of the numerical simulation of forging was to verify the existing procedure of forging a plate from a conventional polygonal 8K forging ingot and a slab ingot with a polygonal shape of P40N surfaces. The effect of the shape of the ingot on the achievement of the required forging reduction and strain after the cross section of the forging of the plate, with final dimensions of approximately 1010 mm width × 310 mm thickness × 5350 mm length, was studied. The results obtained in the operational experiment showed satisfactory qualitative parameters of the steel forging from the slab P40N ingot which were in accordance with the predicted results of numerical simulations. The results indicated that in selected cases the use of a slab P40N ingot instead of the conventional polygonal 8K forging ingot can be considered in the production of certain plate-type forgings.
Preparation of W-Plated Diamond and Improvement of Thermal Conductivity of Diamond-WC-Cu Composite
Authors: Wang Xulei , He Xinbo , Xu Zhiyang , Qu Xuanhui
Published: March 2021

The tungsten (W)-plated diamond process was explored and optimized. A dense and uniform tungsten coating with a thickness of 900 nm was successfully prepared by the powder covering sintering method. The Diamond-WC-Cu composite with high density and high thermal conductivity were successfully prepared by cyclic vacuum pressure infiltration. The microstructure and composition of the W-plated diamond particles were analyzed. The effect of tungsten coating on the microstructure and thermal conductivity of the Diamond-WC-Cu composite was investigated. After calculation, the interface thermal resistance of the composite forming the tungsten carbide transition layer is 2.11 × 10−8 m2∙K∙W−1. The thermal conductivity average value of the Diamond-WC-Cu composite with a diamond volume fraction of 60% reaches 874 W∙m−1∙K−1, which is close to the theoretical prediction value of Hasselman-Johnson (H-J) model and differential effective medium (DEM) model. Moreover, the Maxwell-Eucken (M-E) model, H-J model, and DEM model were used to evaluate the thermal conductivity of the Diamond-WC-Cu composite.
Hot Rolling of Magnesium Single Crystals
Authors: Estrada-Martínez José Antonio , Hernández-Silva David , Al-Samman Talal
Published: March 2021

To analyze the effect of the initial orientation in the activity of twinning and texture development, magnesium single crystals were rolled at 400 °C (nominal furnace temperature) in two specific orientations. In both orientations, the rolling direction of the sheet (RD) was parallel to the c-axis. For orientation 1, the 112¯0 direction was parallel to the normal direction (ND), and for orientation 2, it was parallel to the 101¯0 direction. The samples were rolled at 30%, 50% and 80% of thickness reduction. After rolling, all the samples were quenched in water to retain the microstructure. The microstructure and texture evolution were characterized by X-ray diffraction and Electron Backscatter Diffraction (EBSD). The initial single crystals were turned into polycrystals, where most grains had their c-axis almost parallel to the ND, and this reorientation was explained by extension twinning. The active twin variants in orientation 1 aligned the basal plane ~30° from the sheet plane and caused a weaker basal texture compared to orientation 2, where the twin variants aligned the basal plane almost parallel to the sheet plane. Strain localization inside contraction twins was observed, and consequently, non-basal grains nucleated inside these twins and weakened the final basal texture only in orientation 1.