bluuebunny/crossref_metadata_2025 huggingface dataset:
Eggshell quality: a comparison between Fayoumi, Gimieizah and Brown Hy-Line strains for mechanical properties and ultrastructure of their eggshells
Authors: Radwan Lamiaa M.
Published: February 2015

This experiment was designed to study the relationship between mechanical properties and ultrastructure of three genetic groups (Fayoumi, Gimieizah and Brown Hy-Line strains). To assess eggshell mechanical parameters a total of 120 eggs from each strain was used. This study suggested predicting the mechanical properties and ultrastructure of eggshells when egg weight was equal for the three strains (39.8 g). There was a significant difference among strains for shell thickness, eggshell breaking strength and stiffness, with the Fayoumi eggshells recording the highest values compared with other strains. The palisade layer of Gimieizah and Brown Hy-Line eggshells was thinner than that of Fayoumi eggshells. There were significant correlations between palisade length and breaking strength (0.51), shell thickness (0.89) and stiffness (0.48); however, the length of the palisade layer was significantly negatively correlated with elasticity (–0.85). The Fayoumi (local Egyptian strain) showed higher resistance for eggshell breakage than the Hy-Line commercial strain due to their longer palisade layer noting that there were no significant differences in egg weight between the two. The length of the palisade layer plays an important role in eggshell strength, so it is of interest to include these parameters in selection programs aimed to improve eggshell strength. However, a negative correlation between length of palisade layer and elasticity prevented improvement of these two parameters.
Comparative developmental anatomy of the taproot of the cucurbitaceous vines Citrullus colocynthis (perennial), Citrullus lanatus (annual) and Cucumis myriocarpus (annual)
Authors: Burrows Geoffrey E. , Shaik Razia S.
Published: February 2015

The genus Citrullus (Cucurbitaceae) consists of four species of desert vines. Two species (Citrullus colocynthis and Citrullus lanatus) are widespread weeds on several continents. Above ground, they can be relatively difficult to distinguish apart. However, Citrullus colocynthis is a perennial with a tuberous taproot, whereas Citrullus lanatus is an annual with a slender taproot. We studied the morphology and anatomy of taproot development to better understand their structural and ecological differences. The annual Citrullus lanatus reached close to its maximum taproot diameter (~3 mm) soon after germination. The vascular cambium formed four relatively broad triangular sectors of fibres in which were embedded relatively large diameter vessels. These sectors were separated by narrower triangular areas of secondary ray parenchyma. In contrast, the taproot diameter of the perennial Citrullus colocynthis continued to increase during the study, reaching ~20 mm after 14 weeks. Most of this substantial root consisted of secondary xylem parenchyma, with a low density of relatively small diameter vessels and few fibres. The remarkable differences in root morphology and anatomy of the studied species of Citrullus are related to differences in their annual and perennial lifecycles. Interestingly, the slender taproots of Citrullus lanatus were calculated to have a similar theoretical hydraulic conductance to that of Citrullus colocynthis (large diameter taproot).
Genetic diversity within a population of Microlaena stipoides, as revealed by AFLP markers
Authors: Mitchell M. L. , Stodart B. J. , Virgona J. M.
Published: February 2015

Microlaena stipoides (Labill.) R.Br. (microlaena), a C3 perennial grass, is common within grazed native pastures in the high-rainfall zone (>550 mm average annual rainfall) of south-eastern Australia. It has the ability to spread via seed production or vegetatively, using both rhizomes and stolons. This experiment aimed to determine how variable a microlaena population was within a single area, with the aim of determining whether microlaena relied on seed or vegetative spread to sustain populations. Leaf samples of microlaena were collected from 85 locations, sampling two transects, within a pasture at Chiltern, in north-eastern Victoria (36°12ʹS, 146°35ʹE). The genetic diversity among samples was analysed using amplified fragment length polymorphism (AFLP) markers. We obtained 1612 fragments, using 10 primers combinations. Polymorphism for the markers ranged from 47% to 65%. These results indicated that the populations of microlaena that exist within the pasture at Chiltern are likely to have undergone some degree of outcrossing (Fst = 0.0219). It is likely that recruitment is occurring from sexual reproduction as well as via clonal spread within the microlaena population examined. This ability to use vegetative spread as well as both sexual and asexual reproduction may make populations of microlaena more resilient in the longer term.
Confirming the genetic affinity of the ‘Eyres Green’ saltbush cultivar as oldman saltbush (Atriplex nummularia Lindl.)
Authors: Sampson J. F. , Byrne M. , Norman H. C. , Barrett-Lennard E.
Published: February 2015

Until recently, ‘Eyres Green’ was the only commercial cultivar of oldman saltbush available for forage planting and landscape rehabilitation. ‘Eyres Green’ is believed to be a clonal cultivar of the ecologically and economically important octoploid dioecious species Atriplex nummularia (Lindl.), but its actual identity is unknown. The genetic relationship of the ‘Eyres Green’ clonal cultivar to the two subspecies A. nummularia ssp. nummularia and A. nummularia ssp. spathulata (Aellen) was assessed using nuclear microsatellite markers, principal coordinate analysis and Bayesian clustering. ‘Eyres Green’ clustered with ssp. nummularia in all analyses, suggesting that the cultivar was derived from an individual of this subspecies, probably from the north-eastern part of the subspecies’ distribution in eastern Australia. Atriplex species are dioecous and substantially wind-pollinated. Plants of the ‘Eyres Green’ cultivar are female, so risk of genetic contamination from initial plantings is low, but could occur if plantings within pollination distance of natural populations set seed and establish male plants that subsequently become a source of pollen contamination.
Residency and movement dynamics of southern rock lobster (Jasus edwardsii) after a translocation event
Authors: Linnane Adrian , Penny Shane , Hawthorne Peter , Hoare Matthew
Published: February 2015

Previous movement studies on the southern rock lobster (Jasus edwardsii) have all involved releasing tagged animals at the point of capture. In 2007, 5298 lobsters, in total, were tagged and translocated from an offshore site (>100-m depth) to two inshore sites (<20-m depth) in South Australia. After a period of 735 days, 510 (9.6%) had been recaptured. The majority of translocated lobsters were located within close proximity to the release points, with 306 (60%) having moved <5km. Of the remainder, 133 (26%) were recaptured within 5–10km, with a further 71 (14%) individuals having moved >10km. Movement patterns were highly directional in nature, with individuals consistently travelling in a south-west bearing, regardless of distance moved. In almost all cases, movement was from inshore to offshore sites, with female lobsters travelling significantly further (mean 5.66km ±6.41s.d.) than males (mean 5.02km ±9.66s.d.). The results are consistent with previous large-scale tagging studies of J. edwardsii, which indicated high residency levels but with occasional directed movement by some individuals.
Influence of industrial light pollution on the sea-finding behaviour of flatback turtle hatchlings
Authors: Kamrowski Ruth L. , Limpus Col , Pendoley Kellie , Hamann Mark
Published: February 2015

Context Numerous studies show that artificial light disrupts the sea-finding ability of marine turtle hatchlings. Yet very little has been published regarding sea-finding for flatback turtles. Given the current industrialisation of Australia’s coastline, and the large potential for disruption posed by industrial light, this study is a timely investigation into sea-finding behaviour of flatback turtle hatchlings. Aims We investigate sea-finding by flatback turtle hatchlings in relation to ambient light present in areas of planned or ongoing industrial development, and evaluate the fan and arena-based methods that are frequently used for quantifying hatchling dispersion. Methods Using a combination of methods, we assessed the angular range and directional preference of sea-finding hatchlings at two key flatback turtle rookeries, Peak and Curtis Islands, during January–February 2012 and 2013, and at Curtis Island in January 2014. Relative light levels at each site were measured using an Optec SSP-3 stellar photometer, and moon phase, moon stage and cloud cover were also recorded. Key results We found no evidence of impaired hatchling orientation, and observed very low levels of light at Peak Island. However, at Curtis Island, hatchlings displayed reduced sea-finding ability, with light horizons from the direction of nearby industry significantly brighter than from other directions. The sea-finding disruption observed at Curtis Island was less pronounced in the presence of moonlight. Conclusions The reduced sea-finding ability of Curtis Island hatchlings was likely due to both altered light horizons from nearby industry, as well as beach topography. Both methods of assessing hatchling orientation have benefits and limitations. We suggest that fan-based methods, combined with strategically placed arenas, would provide the best data for accurately assessing hatchling sea-finding. Implications Sky glow produced by large-scale industrial development appears detrimental to sea-finding by flatback turtle hatchlings. As development continues around Australia’s coastline, we strongly recommend continued monitoring of lighting impacts at adjacent turtle nesting beaches. We also advise rigorous management of industrial lighting, which considers cumulative light levels in regions of multiple light producers, as well as moon phase, moon-stage, cloud cover and time of hatchling emergence. All these factors affect the likelihood of disrupted hatchling sea-finding behaviour at nesting beaches exposed to artificial light-glow, industrial or otherwise.
How useful are corpus-based methods for extrapolating psycholinguistic variables?
Authors: Mandera Paweł , Keuleers Emmanuel , Brysbaert Marc
Published: February 2015

Subjective ratings for age of acquisition, concreteness, affective valence, and many other variables are an important element of psycholinguistic research. However, even for well-studied languages, ratings usually cover just a small part of the vocabulary. A possible solution involves using corpora to build a semantic similarity space and to apply machine learning techniques to extrapolate existing ratings to previously unrated words. We conduct a systematic comparison of two extrapolation techniques: k-nearest neighbours, and random forest, in combination with semantic spaces built using latent semantic analysis, topic model, a hyperspace analogue to language (HAL)-like model, and a skip-gram model. A variant of the k-nearest neighbours method used with skip-gram word vectors gives the most accurate predictions but the random forest method has an advantage of being able to easily incorporate additional predictors. We evaluate the usefulness of the methods by exploring how much of the human performance in a lexical decision task can be explained by extrapolated ratings for age of acquisition and how precisely we can assign words to discrete categories based on extrapolated ratings. We find that at least some of the extrapolation methods may introduce artefacts to the data and produce results that could lead to different conclusions that would be reached based on the human ratings. From a practical point of view, the usefulness of ratings extrapolated with the described methods may be limited.
Sport as a critique of politics: Everest climbing, nationalism and the failure of politics in Bangladesh
Authors: Hasan Mubashar
Published: February 2015

Attempting to scale Mount Everest is a very recent sporting activity for Bangladeshis. Presently, four Bangladeshis (two men and two women) have hoisted the flag of Bangladesh, a country ranked consistently low on global governance and development indices, over the peak of Everest. In the aftermath, these mountaineers have become ‘sources of national inspiration’ and received national media attention and have been associated with politicians and civil society groups. In other words, they become national heroes – powerful symbols of nationalism – in recognition of their ‘heroic’ achievement of hoisting the flag at the top of the world. My thesis is that, in Bangladesh, Everest climbing has political significance as these mountaineers use their public platform to criticise national politics. Even hoisting the flag at the top of the world (after risking own lives) can be used as a way to express dissatisfaction over the country’s politics.
An Empirical Analysis of “Tort Tales”
Authors: Malhotra Neil
Published: February 2015

This research note investigates how “tort tales”—cultural memes about the American civil legal system—affect citizens’ attitudes on tort reform. While legal scholarship has extensively analyzed “tort tales” using qualitative approaches, this analysis introduces quantitative methods from political psychology. I explore a case study of Stella Liebeck, the woman who spilled coffee on herself and successfully sued McDonald’s. An experiment embedded in an original survey of 742 Americans shows that exogenously providing people with information about the legal urban legend increased support for tort reform. Further, those with incorrect interpretations of the story are most supportive of tort reform initiatives.
Emerging Roles for Histone Deacetylases in Pulmonary Hypertension and Right Ventricular Remodeling (2013 Grover Conference series)
Authors: Cavasin Maria A. , Stenmark Kurt R. , McKinsey Timothy A.
Published: February 2015

Reversible lysine acetylation has emerged as a critical mechanism for controlling the function of nucleosomal histones as well as diverse nonhistone proteins. Acetyl groups are conjugated to lysine residues in proteins by histone acetyltransferases and removed by histone deacetylases (HDACs), which are also commonly referred to as lysine deacetylases. Over the past decade, many studies have shown that HDACs play crucial roles in the control of left ventricular (LV) cardiac remodeling in response to stress. Small molecule HDAC inhibitors block pathological hypertrophy and fibrosis and improve cardiac function in various preclinical models of LV failure. Only recently have HDACs been studied in the context of right ventricular (RV) failure, which commonly occurs in patients who experience pulmonary hypertension (PH). Here, we review recent findings with HDAC inhibitors in models of PH and RV remodeling, propose next steps for this newly uncovered area of research, and highlight potential for isoform‐selective HDAC inhibitors for the treatment of PH and RV failure.
Multi-dimensional stability of waves travelling through rectangular lattices in rational directions
Authors: Hoffman A. , Hupkes H. , Van Vleck E.
Published: February 2015

We consider general reaction diffusion systems posed on rectangular lattices in two or more spatial dimensions. We show that travelling wave solutions to such systems that propagate in rational directions are nonlinearly stable under small perturbations. We employ recently developed techniques involving pointwise Green’s functions estimates for functional differential equations of mixed type (MFDEs), allowing our results to be applied even in situations where comparison principles are not available.
Distinct self-interaction domains promote Multi Sex Combs accumulation in and formation of theDrosophilahistone locus body
Authors: Terzo Esteban A. , Lyons Shawn M. , Poulton John S. , Temple Brenda R. S. , Marzluff William F. , Duronio Robert J.
Published: February 2015

Nuclear bodies (NBs) are structures that concentrate proteins, RNAs, and ribonucleoproteins that perform functions essential to gene expression. How NBs assemble is not well understood. We studied the Drosophila histone locus body (HLB), a NB that concentrates factors required for histone mRNA biosynthesis at the replication-dependent histone gene locus. We coupled biochemical analysis with confocal imaging of both fixed and live tissues to demonstrate that the Drosophila Multi Sex Combs (Mxc) protein contains multiple domains necessary for HLB assembly. An important feature of this assembly process is the self-interaction of Mxc via two conserved N-terminal domains: a LisH domain and a novel self-interaction facilitator (SIF) domain immediately downstream of the LisH domain. Molecular modeling suggests that the LisH and SIF domains directly interact, and mutation of either the LisH or the SIF domain severely impairs Mxc function in vivo, resulting in reduced histone mRNA accumulation. A region of Mxc between amino acids 721 and 1481 is also necessary for HLB assembly independent of the LisH and SIF domains. Finally, the C-terminal 195 amino acids of Mxc are required for recruiting FLASH, an essential histone mRNA-processing factor, to the HLB. We conclude that multiple domains of the Mxc protein promote HLB assembly in order to concentrate factors required for histone mRNA biosynthesis.
Cytoplasmic hGle1A regulates stress granules by modulation of translation
Authors: Aditi  , Folkmann Andrew W. , Wente Susan R.
Published: February 2015

When eukaryotic cells respond to stress, gene expression pathways change to selectively export and translate subsets of mRNAs. Translationally repressed mRNAs accumulate in cytoplasmic foci known as stress granules (SGs). SGs are in dynamic equilibrium with the translational machinery, but mechanisms controlling this are unclear. Gle1 is required for DEAD-box protein function during mRNA export and translation. We document that human Gle1 (hGle1) is a critical regulator of translation during stress. hGle1 is recruited to SGs, and hGLE1 small interfering RNA–mediated knockdown perturbs SG assembly, resulting in increased numbers of smaller SGs. The rate of SG disassembly is also delayed. Furthermore, SG hGle1-depletion defects correlate with translation perturbations, and the hGle1 role in SGs is independent of mRNA export. Interestingly, we observe isoform-specific roles for hGle1 in which SG function requires hGle1A, whereas mRNA export requires hGle1B. We find that the SG defects in hGle1-depleted cells are rescued by puromycin or DDX3 expression. Together with recent links of hGLE1 mutations in amyotrophic lateral sclerosis patients, these results uncover a paradigm for hGle1A modulating the balance between translation and SGs during stress and disease.
The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia
Authors: Urbanska Paulina , Song Kangkang , Joachimiak Ewa , Krzemien-Ojak Lucja , Koprowski Piotr , Hennessey Todd , Jerka-Dziadosz Maria , Fabczak Hanna , Gaertig Jacek , Nicastro Daniela , Wloga Dorota
Published: February 2015

Dynein motors and regulatory complexes repeat every 96 nm along the length of motile cilia. Each repeat contains three radial spokes, RS1, RS2, and RS3, which transduct signals between the central microtubules and dynein arms. Each radial spoke has a distinct structure, but little is known about the mechanisms of assembly and function of the individual radial spokes. In Chlamydomonas, calmodulin and spoke-associated complex (CSC) is composed of FAP61, FAP91, and FAP251 and has been linked to the base of RS2 and RS3. We show that in Tetrahymena, loss of either FAP61 or FAP251 reduces cell swimming and affects the ciliary waveform and that RS3 is either missing or incomplete, whereas RS1 and RS2 are unaffected. Specifically, FAP251-null cilia lack an arch-like density at the RS3 base, whereas FAP61-null cilia lack an adjacent portion of the RS3 stem region. This suggests that the CSC proteins are crucial for stable and functional assembly of RS3 and that RS3 and the CSC are important for ciliary motility.
MiR-1188 at the imprintedDlk1-Dio3domain acts as a tumor suppressor in hepatoma cells
Authors: Cui Wei , Huang Zhijun , He Hongjuan , Gu Ning , Qin Geng , Lv Jie , Zheng Tao , Sugimoto Kenkichi , Wu Qiong
Published: February 2015

The aberrant expression of microRNAs (miRNAs) has frequently been reported in cancer studies; miRNAs play roles in development, progression, metastasis, and prognosis. Recent studies indicate that the miRNAs within the Dlk1-Dio3 genomic region are involved in the development of liver cancer, but the role of miR-1188 in hepatocellular carcinoma (HCC) and the pathway by which it exerts its function remain largely unknown. Here we demonstrate that miR-1188 is significantly down-regulated in mouse hepatoma cells compared with normal liver tissues. Enhanced miR-1188 suppresses cell proliferation, migration, and invasion in vitro and inhibits the tumor growth of HCC cells in vivo. Moreover, overexpressed miR-1188 promotes apoptosis, enhances caspase-3 activity, and also up-regulates the expression of Bax and p53. MiR-1188 directly targets and negatively regulates Bcl-2 and Sp1. Silencing of Bcl-2 and Sp1 exactly copies the proapoptotic and anti-invasive effects of miR-1188, respectively. The expression of apoptosis- and invasion-related genes, such as Vegfa, Fgfr1, and Rprd1b, decreases after enhancement of miR-1188, as determined by gene expression profiling analysis. Taken together, our results highlight an important role for miR-1188 as a tumor suppressor in hepatoma cells and imply its potential role in cancer therapy.
A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue
Authors: Merulla Jessica , Soldà Tatiana , Molinari Maurizio
Published: February 2015

Only native polypeptides are released from the endoplasmic reticulum (ER) to be transported at the site of activity. Persistently misfolded proteins are retained and eventually selected for ER-associated degradation (ERAD). The paradox of a structure-based protein quality control is that functional polypeptides may be destroyed if they are architecturally unfit. This has health-threatening implications, as shown by the numerous “loss-of-function” proteopathies, but also offers chances to intervene pharmacologically to promote bypassing of the quality control inspection and export of the mutant, yet functional protein. Here we challenged the ER of human cells with four modular glycopolypeptides designed to alert luminal and membrane protein quality checkpoints. Our analysis reveals the unexpected collaboration of the cytosolic AAA-ATPase p97 and the luminal quality control factor UDP-glucose:glycoprotein glucosyltransferase (UGGT1) in a novel, BiP- and CNX-independent checkpoint. This prevents Golgi transport of a chimera with a native ectodomain that passes the luminal quality control scrutiny but displays an intramembrane defect. Given that human proteopathies may result from impaired transport of functional polypeptides with minor structural defects, identification of quality checkpoints and treatments to bypass them as shown here upon silencing or pharmacologic inhibition of UGGT1 or p97 may have important clinical implications.
The effect of parental size on spermatophore production, egg quality, fertilization success, and larval characteristics in the Caribbean Spiny lobster, Panulirus argus
Authors: Butler Mark J. , Macdiarmid Alison , Gnanalingam Gaya
Published: February 2015

The average size of spiny lobsters (Decapoda; Palinuridae) has decreased worldwide over the past few decades. Market forces coupled with minimum size limits compel fishers to target the largest individuals. Males are targeted disproportionately as a consequence of sexual dimorphism in spiny lobster size (i.e. males grow larger than females) and because of protections for ovigerous females. Therefore, overexploitation of males has led to sperm limitation in several decapod populations with serious repercussions for reproductive success. In the Caribbean spiny lobster, Panulirus argus, little is known about the effect of reduced male size on fertilization success or the role that individual size plays in gamete and larval quality. We conducted a series of laboratory experiments to test the relationship between male size and spermatophore production over multiple mating events and to determine whether spermatophore reduction and female size affected fertilization success or larval attributes in P. argus in the Florida Keys, FL (USA). We found that over consecutive matings, larger males consistently produced spermatophores of a greater weight and area than smaller males, although size-specific differences in sperm cell density were undetected and probably obscured by high variance in the data. Where spermatophores were experimentally reduced to mimic the decline in spermatophore size with declining male size, fertilization success (the number of fertilized eggs/total number of eggs extruded) declined, indicating that sperm availability is indeed limited. No maternal size effects on egg size or quality (C:N ratio) or larval quality (size, swimming speed, mortality) were observed. Our results demonstrate the importance of maintaining large males in populations of P. argus to ensure fertilization success and caution against their overexploitation through fishing, which may severely reduce reproductive success and thus population sustainability.
Genetic and Clinical Factors Associated with Chronic Postsurgical Pain after Hernia Repair, Hysterectomy, and Thoracotomy
Authors: Montes Antonio , Roca Gisela , Sabate Sergi , Lao Jose Ignacio , Navarro Arcadi , Cantillo Jordi , Canet Jaume
Published: February 2015

AbstractAbstract

Persistent postoperative pain was diagnosed in 18% of a population-based sample of 2,929 patients who had hernia repairs, hysterectomies, or thoracotomies. The association of persistent pain with 90 genetic markers showed no evidence for genetic predisposition in a subset of 1,000 patients. Six clinical factors predicted 73% of the persistent pain that developed.

Supplemental Digital Content is available in the text.

Background:

Chronic postsurgical pain (CPSP) has been linked to many surgical settings. The authors aimed to analyze functional genetic polymorphisms and clinical factors that might identify CPSP risk after inguinal hernia repair, hysterectomy, and thoracotomy.

Methods:

This prospective multicenter cohort study enrolled 2,929 patients scheduled for inguinal hernia repair, hysterectomy (vaginal or abdominal), or thoracotomy. The main outcome was the incidence of CPSP confirmed by physical examination 4 months after surgery. The secondary outcome was CPSP incidences at 12 and 24 months. The authors also tested the associations between CPSP and 90 genetic markers plus a series of clinical factors and built a CPSP risk model.

Results:

Within a median of 4.4 months, CPSP had developed in 527 patients (18.0%), in 13.6% after hernia repair, 11.8% after vaginal hysterectomy, 25.1% after abdominal hysterectomy, and 37.6% after thoracotomy. CPSP persisted after a median of 14.6 months and 26.3 months in 6.2% and 4.1%, respectively, after hernia repair, 4.1% and 2.2% after vaginal hysterectomy, 9.9% and 6.7% after abdominal hysterectomy, and 19.1% and 13.2% after thoracotomy. No significant genetic differences between cases and controls were identified. The risk model included six clinical predictors: (1) surgical procedure, (2) age, (3) physical health (Short Form Health Survey-12), (4) mental health (Short Form Health Survey-12), (5) preoperative pain in the surgical field, and (6) preoperative pain in another area. Discrimination was moderate (c-statistic, 0.731; 95% CI, 0.705 to 0.755).

Conclusions:

Until unequivocal genetic predictors of CPSP are understood, the authors encourage systematic use of clinical factors for predicting and managing CPSP risk.
Blunt Intraoral Trauma Resulting in Internal Carotid Artery Dissection and Infarction in a Child
Authors: Bent Chris , Shen Peter , Dahlin Brian , Coulter Kevin
Published: February 2015

A 16-month-old child fell forward onto her toothbrush sustaining minor oropharyngeal injury. The following day, she became acutely lethargic with localizing neurologic signs of a cerebrovascular infarct. CTA and MR imaging demonstrated occlusion of the right internal carotid artery with a large right middle cerebral artery territory infarction. She was treated with decompressive craniectomy and anticoagulation but remained weak on the left side. Pediatric oropharyngeal injuries can rarely be complicated by internal carotid artery injury with dissection, thrombosis, or embolization to the cerebral circulation. For the best outcome, carotid dissection treatment requires prompt diagnosis at the initial onset of neurologic symptoms. However, further research is needed to determine the best management and advanced imaging work-up for neurologically intact children.
Scaling and universality in the human voice
Authors: Luque Jordi , Luque Bartolo , Lacasa Lucas
Published: February 2015

Speech is a distinctive complex feature of human capabilities. In order to understand the physics underlying speech production, in this work, we empirically analyse the statistics of large human speech datasets ranging several languages. We first show that during speech, the energy is unevenly released and power-law distributed, reporting a universal robust Gutenberg–Richter-like law in speech. We further show that such ‘earthquakes in speech’ show temporal correlations, as the interevent statistics are again power-law distributed. As this feature takes place in the intraphoneme range, we conjecture that the process responsible for this complex phenomenon is not cognitive, but it resides in the physiological (mechanical) mechanisms of speech production. Moreover, we show that these waiting time distributions are scale invariant under a renormalization group transformation, suggesting that the process of speech generation is indeed operating close to a critical point. These results are put in contrast with current paradigms in speech processing, which point towards low dimensional deterministic chaos as the origin of nonlinear traits in speech fluctuations. As these latter fluctuations are indeed the aspects that humanize synthetic speech, these findings may have an impact in future speech synthesis technologies. Results are robust and independent of the communication language or the number of speakers, pointing towards a universal pattern and yet another hint of complexity in human speech.
Transitional flow in aneurysms and the computation of haemodynamic parameters
Authors: Poelma Christian , Watton Paul N. , Ventikos Yiannis
Published: February 2015

Haemodynamic forces appear to play an influential role in the evolution of aneurysms. This has led to numerous studies, usually based on computational fluid dynamics. Their focus is predominantly on the wall shear stress (WSS) and associated derived parameters, attempting to find correlations between particular patterns of haemodynamic indices and regions subjected to disease formation and progression. The indices are generally determined by integration of flow properties over a single cardiac cycle. In this study, we illustrate that in some cases the transitional flow in aneurysms can lead to significantly different WSS distributions in consecutive cardiac cycles. Accurate determination of time-averaged haemodynamic indices may thus require simulation of a large number of cycles, which contrasts with the common approach to determine parameters using data from a single cycle. To demonstrate the role of transitional flow, two exemplary cases are considered: flow in an abdominal aortic aneurysm and in an intracranial aneurysm. The key differences that are observed between these cases are explained in terms of the integral timescale of the transitional flows in comparison with the cardiac cycle duration: for relatively small geometries, transients will decay before the next cardiac cycle. In larger geometries, transients are still present when the systolic phase produces new instabilities. These residual fluctuations serve as random initial conditions and thus seed different flow patterns in each cycle. To judge whether statistics are converged, the derived indices from at least two successive cardiac cycles should be compared.
The RED domain of Paired is specifically required forDrosophilaaccessory gland maturation
Authors: Li Li , Li Ping , Xue Lei
Published: February 2015

The evolutionarily conserved paired domain consists of the N-terminal PAI and the C-terminal RED domains, each containing a helix–turn–helix motif capable of binding DNA. Despite its conserved sequence, the physiological functions of the RED domain remain elusive. Here, we constructed aprdtransgene expressing a truncated Paired (Prd) protein without the RED domain, and examined its rescue ability inprdmutants. We found that the RED domain is specifically required for the expression of Acp26Aa and sex peptide in male accessory glands, and the induction of female post-mating response. Our data thus identified an important physiological function for the evolutionarily conserved RED domain.
A spectacular new species of seadragon (Syngnathidae)
Authors: Stiller Josefin , Wilson Nerida G. , Rouse Greg W.
Published: February 2015

The exploration of Earth's biodiversity is an exciting and ongoing endeavour. Here, we report a new species of seadragon from Western Australia with substantial morphological and genetic differences to the only two other known species. We describe it as
Phyllopteryx dewysea
n. sp. Although the leafy seadragon (
Phycodurus eques
) and the common seadragon (
Phyllopteryx taeniolatus
) occur along Australia's southern coast, generally among relatively shallow macroalgal reefs, the new species was found more offshore in slightly deeper waters. The holotype was trawled east of the remote Recherche Archipelago in 51 m; additional specimens extend the distribution west to Perth in 72 m. Molecular sequence data show clear divergence from the other seadragons (7.4–13.1% uncorrected divergence in mitochondrial DNA) and support a placement as the sister-species to the common seadragon. Radiographs and micro-computed tomography were used on the holotype of the new species and revealed unique features, in addition to its unusual red coloration. The discovery provides a spectacular example of the surprises still hidden in our oceans, even in relatively shallow waters.
Genetic evidence for an origin of the Armenians from Bronze Age mixing of multiple populations
Authors: Haber Marc , Mezzavilla Massimo , Xue Yali , Comas David , Gasparini Paolo , Zalloua Pierre , Tyler-Smith Chris
Published: February 2015

The Armenians are a culturally isolated population who historically inhabited a region in the Near East bounded by the Mediterranean and Black seas and the Caucasus, but remain underrepresented in genetic studies and have a complex history including a major geographic displacement during World War One. Here, we analyse genome-wide variation in 173 Armenians and compare them to 78 other worldwide populations. We find that Armenians form a distinctive cluster linking the Near East, Europe, and the Caucasus. We show that Armenian diversity can be explained by several mixtures of Eurasian populations that occurred between ~3,000 and ~2,000 BCE, a period characterized by major population migrations after the domestication of the horse, appearance of chariots, and the rise of advanced civilizations in the Near East. However, genetic signals of population mixture cease after ~1,200 BCE when Bronze Age civilizations in the Eastern Mediterranean world suddenly and violently collapsed. Armenians have since remained isolated and genetic structure within the population developed ~500 years ago when Armenia was divided between the Ottomans and the Safavid Empire in Iran. Finally, we show that Armenians have higher genetic affinity to Neolithic Europeans than other present-day Near Easterners, and that 29% of the Armenian ancestry may originate from an ancestral population best represented by Neolithic Europeans.
Structures ofEscherichia colitryptophanase in holo and `semi-holo' forms
Authors: Kogan Anna , Raznov Leah , Gdalevsky Garik Y. , Cohen-Luria Rivka , Almog Orna , Parola Abraham H. , Goldgur Yehuda
Published: February 2015

Two crystal forms ofEscherichia colitryptophanase (tryptophan indole-lyase, Trpase) were obtained under the same crystallization conditions. Both forms belonged to the same space groupP43212 but had slightly different unit-cell parameters. The holo crystal form, with pyridoxal phosphate (PLP) bound to Lys270 of both polypeptide chains in the asymmetric unit, diffracted to 2.9 Å resolution. The second crystal form diffracted to 3.2 Å resolution. Of the two subunits in the asymmetric unit, one was found in the holo form, while the other appeared to be in the apo form in a wide-open conformation with two sulfate ions bound in the vicinity of the active site. The conformation of all holo subunits is the same in both crystal forms. The structures suggest that Trpase is flexible in the apo form. Its conformation partially closes upon binding of PLP. The closed conformation might correspond to the enzyme in its active state with both cofactor and substrate bound in a similar way as in tyrosine phenol-lyase.
Mode of binding of the antithyroid drug propylthiouracil to mammalian haem peroxidases
Authors: Singh R. P. , Singh A. , Kushwaha G. S , Singh A. K. , Kaur P. , Sharma S. , Singh T. P.
Published: February 2015

The mammalian haem peroxidase superfamily consists of myeloperoxidase (MPO), lactoperoxidase (LPO), eosinophil peroxidase (EPO) and thyroid peroxidase (TPO). These enzymes catalyze a number of oxidative reactions of inorganic substrates such as Cl−, Br−, I−and SCN−as well as of various organic aromatic compounds. To date, only structures of MPO and LPO are known. The substrate-binding sites in these enzymes are located on the distal haem side. Propylthiouracil (PTU) is a potent antithyroid drug that acts by inhibiting the function of TPO. It has also been shown to inhibit the action of LPO. However, its mode of binding to mammalian haem peroxidases is not yet known. In order to determine the mode of its binding to peroxidases, the structure of the complex of LPO with PTU has been determined. It showed that PTU binds to LPO in the substrate-binding site on the distal haem side. The IC50values for the inhibition of LPO and TPO by PTU are 47 and 30 µM, respectively. A comparision of the residues surrounding the substrate-binding site on the distal haem side in LPO with those in TPO showed that all of the residues were identical except for Ala114 (LPO numbering scheme), which is replaced by Thr205 (TPO numbering scheme) in TPO. A threonine residue in place of alanine in the substrate-binding site may affect the affinity of PTU for peroxidases.
Crystallization and preliminary X-ray analysis of thePlasmodium falciparumapicoplast DNA polymerase
Authors: Milton Morgan E. , Choe Jun-yong , Honzatko Richard B. , Nelson Scott W.
Published: February 2015

Infection by the parasitePlasmodium falciparumis the leading cause of malaria in humans. The parasite has a unique and essential plastid-like organelle called the apicoplast. The apicoplast contains a genome that undergoes replication and repair through the action of a replicative polymerase (apPOL). apPOL has no direct orthologs in mammalian polymerases and is therefore an attractive antimalarial drug target. No structural information exists for apPOL, and the Klenow fragment ofEscherichia coliDNA polymerase I, which is its closest structural homolog, shares only 28% sequence identity. Here, conditions for the crystallization of and preliminary X-ray diffraction data from crystals ofP. falciparumapPOL are reported. Data complete to 3.5 Å resolution were collected from a single crystal (2 × 2 × 5 µm) using a 5 µm beam. The space groupP6522 (unit-cell parametersa=b= 141.8,c= 149.7 Å, α = β = 90, γ = 120°) was confirmed by molecular replacement. Refinement is in progress.
A transformative approach to work integrated learning in legal education
Authors: Babacan Alperhan , Babacan Hurriyet
Published: February 2015

Purpose

– The purpose of this paper is to discuss the current context, scope and problems in the provision of work-integrated learning (WIL) in legal education and how the adoption transformative pedagogies in WIL which is offered in legal education can foster personal and social transformation in addition to enhancing lawyering skills. The paper draws on learning from Australia, England and the USA.

Design/methodology/approach

– The backdrop of this conceptual paper is WIL and transformative education. The text begins with a critique of existing WIL frameworks and practices in legal education in Australia, England and the USA. This exposes a focus on skills enhancement at the expense of social and personal transformation. Drawing on transformative learning, the paper proposes practices which can be used in WIL offered in legal education to enhance personal and social transformation.

Findings

– There is very little literature on how legal education and WIL in legal education can enhance personal and social transformation. Tensions continue to exist between the predominant aim of instilling the legal skills necessary to ensure that graduates are prepared for legal practice through WIL programmes and between the need to simultaneously enhance critical consciousness and social transformation necessary for active participation in social and professional life.

Research limitations/implications

– More research is required on the best manner in which the ideals and practices of emancipatory education can be installed within WIL programmes so as to successfully reduce the tensions between the instilling of legal skills required to practice law and the need to train students to be holistic, critical and constructive thinkers.

Practical implications

– The suggestions made in this paper provide a framework to adopt critical pedagogies in the provision of WIL in legal education. The theoeretical and practice-based suggestions presented in this paper are also relevant to other professional disciplines where personal transformation is desired.

Originality/value

– The literature on legal education predominantly focuses on enhancing lawyering skills and competencies and there is an absence of the utilisation of transformative pedagogies in legal education generally and WIL offered in legal education. Drawing predominantly on the literature and practices relating to legal education in Australia and incorporating comparative insights from England and the USA, the paper contributes to the broader literature on transformative learning. Most significantly, the paper contributes specifically to the use of transformative pedagogies in WIL offered in legal education through the suggestion of practices relating to critical reflection and dialogue which are not commonly used in legal education.
A longitudinal study of the impact of the Sydney Olympics on real estate markets
Authors: Lu Qiang (Steven) , Yang Yupin
Published: February 2015

Purpose

– The purpose of this paper is to examine the impact of the Sydney 2000 Olympic Games on the residential real estate markets of the host city during the bidding, pre-Olympic and post-Olympic periods.

Design/methodology/approach

– This study uses a difference-in-differences model to analyze the transaction prices for all properties in New South Wales, Australia for the period from 1980 to 2007.

Findings

– The paper finds that the impact on real estate markets varies across different suburbs in the host city and over time. The real estate markets of host suburbs experience substantially higher growth during the bidding and pre-Olympic periods but not during the post-Olympic period. However, the property prices in non-host suburbs in the host city increase at a higher rate during the pre- and post-Olympic periods but not during the bidding period.

Originality/value

– This study offers insights into the long-term impact of the Olympic Games on host suburbs and non-host suburbs in the host city during different periods by analyzing a large longitudinal data set over a period of 27 years.
What determines expatriates’ performance while abroad? The role of job embeddedness
Authors: Andresen Maike
Published: February 2015

Purpose

– The purpose of this paper is to empirically examine job embeddedness as antecedent of job performance and organizational citizenship behaviors (OCBs) of expatriates.

Design/methodology/approach

– In total, 194 expatriates from 39 nationalities were recruited through various expatriate organizations, which provided expatriate groups they had access to with a link to the online survey in English. To test the hypotheses, the author used bivariate analyses and multivariate regressions were calculated to control for alternative explanations.

Findings

– As hypothesized, community embeddedness was positively related to job performance and both organizational embeddedness and community embeddedness were positively related to OCBs. Moreover, community embeddedness and organizational embeddedness showed to be positively related. In addition, organizational embeddedness partially mediated the positive relationship between community embeddedness and job performance for organizational expatriates (moderated mediation) as well as the positive relationship between community embeddedness and OCBs.

Originality/value

– This is one of the first study to address the differential effects of organizational and community embeddedness on job performance in an expatriation context.
The decision-making processes of self-initiated expatriates: a consumer behaviour approach
Authors: Glassock Gabriela , Fee Anthony
Published: February 2015

Purpose

– The purpose of this paper is to explore the features of the decision-making processes used by self-initiated expatriates (SIEs) when considering an international assignment. It does this by examining expatriates’ decision processes through the lens of prominent theories of consumer decision making.

Design/methodology/approach

– An abductive, exploratory research design was employed, based on in-depth qualitative case studies of nine SIEs.

Findings

– In general, the expatriates in the study tended to deploy high-involvement decision-making processes. Rational decision models drawing on multiple high-quality information sources were common, especially for expatriates with career-oriented motivations and no prior experience in the target country. Three types of expatriates are distilled: “career building” (high involvement, career oriented, compensatory decision model), “risk minimizing” (high/medium involvement, non-compensatory decision model), and “emotionally driven” (low involvement, affective decision model).

Originality/value

– While research into expatriates’ motivations is plentiful, this is the first study to examine the decision-making processes that define the way in which these motivations are enacted. Its originality stems from combining two previously unrelated strands of research (consumer decision making and expatriation). The resulting tentative typology of decision-making approaches provides a platform for organisations seeking to better target talent recruitment, and for researchers seeking to further examine the decision processes of SIEs.