bluuebunny/crossref_metadata_2025 huggingface dataset:
Internet Gaming Disorder Increases Mind-Wandering in Young Adults
Authors: Zhang Jiawen , Zhou Hui , Geng Fengji , Song Xiaolan , Hu Yuzheng
Published: January 2021

As a primary symptom defining Internet gaming disorder (IGD), preoccupation indicates a mind state in which gamers think about a gaming activity so much that other things appear less important and/or interesting to them. Previous studies have examined the negative impacts of IGD on both cognitive and affective functions, yet no study has investigated the influence of IGD on daily mind state changes that interfere with ongoing tasks. The current study hypothesized that more IGD symptoms lead to a higher frequency of mind state shift in terms of mind-wandering. As social anxiety is related to both IGD and mind-wandering, we further hypothesized that social anxiety would partially contribute to the relationship. Survey data were collected from 632 young adults who were divided into two groups based on whether they reported playing games or not. In the player group, the number of IGD symptoms present was positively related to mind-wandering (r = 0.269, p < 0.001) and social anxiety (r = 0.235, p < 0.001), with the latter two showing a positive correlation in both players (r = 0.37, p < 0.001) and non-players (r = 0.57, p < 0.001). A mediation analysis for the players showed a partial mediation effect of social anxiety on the relationship between IGD and mind-wandering (mediation effect: PM = 0.292, p < 0.001), and the model was replicated in an independent sample. This study suggests that excessive gaming behavior may increase mind-wandering and a shift towards such a non-productive mind state could exert long-term detrimental effects in adolescents and young adults.
Does Loudness Relate to the Strength of the Sound Produced by the Source or Received by the Ears? A Review of How Focus Affects Loudness
Authors: Berthomieu Gauthier , Koehl Vincent , Paquier Mathieu
Published: January 2021

Loudness is the magnitude of the auditory sensation that a listener experiences when exposed to a sound. Several sound attributes are reported to affect loudness, such as the sound pressure level at the listener's ears and the spectral content. In addition to these physical attributes of the stimulus, some subjective attributes also appear to affect loudness. When presented with a sound, a listener interacts with an auditory object and can focus on several aspects of the latter. Loudness appears to differ depending on how listeners apprehend this object, notably whether they focus on the sound that reaches their ears or that is produced by the source. The way listeners focus on the auditory object may depend on the stimulus itself. For instance, they might be more likely to focus on the sound emitted by the source if the latter is visible. The instructions given by the experimenters can also explicitly direct the listener's focus on the sound reaching the ears or emitted by the source. The present review aims at understanding how listeners focus on the auditory object depending on the stimuli and instructions they are provided with, and to describe how loudness depends on this focus.
Patients and Parents’ Experience of Multi-Family Therapy for Anorexia Nervosa: A Pilot Study
Authors: Baumas Victoria , Zebdi Rafika , Julien-Sweerts Sabrina , Carrot Benjamin , Godart Nathalie , Minier Lisa , Rigal Natalie
Published: January 2021

Background: Family therapy is considered as the gold standard in treatment of adolescents with anorexia nervosa (AN). Among the different types of family therapy, multi-family therapy (MFT) is increasingly used for treating AN, and shows promising results. In this article, our focus relied on the patients’ and their parents’ perceptions of the effectiveness and the underlying mechanisms of the MFT. Methods: The present pilot exploratory qualitative study included two focus groups conducted using a semi-structured approach: one with the adolescents (n= 3), and another with one or two of their parents (n= 4 mothers;n= 2 fathers). The subjects discussed were the changes observed in both AN symptoms and family interactions following therapy, and the mechanisms underlying these changes. We crossed the perspectives of the adolescents and of the parents on these two points. Results: Qualitative analysis revealed that while both adolescents and parents had difficulties relating the changes they observed in the last year to MFT, they were able to say that the group cohesion had several positive effects and that their family dynamics had improved. In the light of analysis the adolescents perceived more improvements related eating disorders symptoms than their parents did, while parents were concerned about a negative effect of MFT on their children. Discussion: While both patients and parents perceived improvements in both AN symptoms and family interactions in the past year, it was not clear if they considered MFT to have led to these improvements. FG also explored the MFT mechanisms underlying changes. Both adolescents and their parents stressed the beneficial effects of identification to others members of the group and shared experience to overcome social isolation. Parents also mentioned the sympathy they felt for each other. The idea that they give a central place to families in the therapy was also described by the families.
How Do Art Skills Influence Visual Search? – Eye Movements Analyzed With Hidden Markov Models
Authors: Tallon Miles , Greenlee Mark W. , Wagner Ernst , Rakoczy Katrin , Frick Ulrich
Published: January 2021

The results of two experiments are analyzed to find out how artistic expertise influences visual search. Experiment I comprised survey data of 1,065 students on self-reported visual memory skills and their ability to find three targets in four images of artwork. Experiment II comprised eye movement data of 50 Visual Literacy (VL) experts and non-experts whose eye movements during visual search were analyzed for nine images of artwork as an external validation of the assessment tasks performed in Sample I. No time constraint was set for completion of the visual search task. A latent profile analysis revealed four typical solution patterns for the students in Sample I, including a mainstream group, a group that completes easy images fast and difficult images slowly, a fast and erroneous group, and a slow working student group, depending on task completion time and on the probability of finding all three targets. Eidetic memory, performance in art education and visual imagination as self-reported visual skills have significant impact on latent class membership probability. We present a hidden Markov model (HMM) approach to uncover underlying regions of attraction that result from visual search eye-movement behavior in Experiment II. VL experts and non-experts did not significantly differ in task time and number of targets found but they did differ in their visual search process: compared to non-experts, experts showed greater precision in fixating specific prime and target regions, assessed through hidden state fixation overlap. Exploratory analysis of HMMs revealed differences between experts and non-experts in image locations of attraction (HMM states). Experts seem to focus their attention on smaller image parts whereas non-experts used wider parts of the image during their search. Differences between experts and non-experts depend on the relative saliency of targets embedded in images. HMMs can determine the effect of expertise on exploratory eye movements executed during visual search tasks. Further research on HMMs and art expertise is required to confirm exploratory results.
The Trade-Off Between Format Familiarity and Word-Segmentation Facilitation in Chinese Reading
Authors: Chen Mingjing , Wang Yongsheng , Zhao Bingjie , Li Xin , Bai Xuejun
Published: January 2021

In alphabetic writing systems (such as English), the spaces between words mark the word boundaries, and the basic unit of reading is distinguished during visual-level processing. The visual-level information of word boundaries facilitates reading. Chinese is an ideographic language whose text contains no intrinsic inter-word spaces as the marker of word boundaries. Previous studies have shown that the basic processing unit of Chinese reading is also a word. However, findings remain inconsistent regarding whether inserting spaces between words in Chinese text promotes reading performance. Researchers have proposed that there may be a trade-off between format familiarity and the facilitation effect of inter-word spaces. In order to verify this, this study manipulated the format familiarity via reversing the Chinese reading direction from right to left to investigate this issue in Experiment 1 and Experiment 2. The purpose of Experiment 1 was to examine whether inter-word spaces facilitated Chinese reading in an unfamiliar format. Experiment 1 was conducted that 40 native Chinese undergraduates read Chinese sentences from right to left on four format conditions. The results showed faster reading speed and shorter total reading time for the inter-word spaced format. Based on this finding, Experiment 2 examined whether the facilitation effect of inter-word spaces would reduce or disappear after improving the format familiarity; this experiment was conducted that 40 native Chinese undergraduates who did not participate in Experiment 1 read Chinese sentences from right to left on four format conditions after ten-day reading training. There was no significant difference between the total reading time and reading speed in the inter-word spaced format and unspaced format, which suggests that the facilitation effect of inter-word spaces in Chinese reading changed smaller. The combined results of the two experiments suggest that there is indeed a trade-off between format familiarity and the facilitation of word segmentation, which supports the assumption of previous studies.
The Gestures in 2–4-Year-Old Children With Autism Spectrum Disorder
Authors: Ye QianYing , Liu LinRu , Lv ShaoLi , Cheng SanMei , Zhu HuiLin , Xu YanTing , Zou XiaoBing , Deng HongZhu
Published: January 2021

Deficits in gestures act as early signs of impairment in social interaction (SI) and communication in children with autism spectrum disorder (ASD). However, the pieces of literature on atypical gesture patterns in ASD children are contradictory. This investigation aimed to explore the atypical gesture pattern of ASD children from the dimensions of quantity, communicative function, and integration ability; and its relationship with social ability and adaptive behavior. We used a semi-structured interactive play to evaluate gestures of 33 ASD children (24–48 months old) and 24 typically developing (TD) children (12–36 months old). And we evaluated the social ability, adaptive behavior, and productive language of ASD and TD children by using the Adaptive Behavior Assessment System version II (ABAS-II) and Chinese Communication Development Inventory (CCDI). No matter the total score of CCDI was corrected or not, the relative frequency of total gestures, behavior regulation (BR) gestures, SI gestures, and joint attention (JA) gestures of ASD children were lower than that of TD children, as well as the proportion of JA gestures. However, there was no significant group difference in the proportion of BR and SI gestures. Before adjusting for the total score of CCDI, the relative frequency of gestures without vocalization/verbalization integration and vocalization/verbalization-integrated gestures in ASD children was lower than that in TD children. However, after matching the total score of CCDI, only the relative frequency of gestures without vocalization/verbalization integration was lower. Regardless of the fact that the total score of CCDI was corrected or not, the relative frequency and the proportion of eye-gaze-integrated gestures in ASD children were lower than that in TD children. And the proportion of gestures without eye-gaze integration in ASD children was higher than that in TD children. For ASD children, the social skills score in ABAS-II was positively correlated with the relative frequency of SI gesture and eye-gaze-integrated gestures; the total score of ABAS-II was positively correlated with the relative frequency of total gestures and eye-gaze-integrated gestures. In conclusion, ASD children produce fewer gestures and have deficits in JA gestures. The deficiency of integrating eye gaze and gesture is the core deficit of ASD children’s gesture communication. Relatively, ASD children might be capable of integrating vocalization/verbalization into gestures. SI gestures and the ability to integrate gesture and eye gaze are related to social ability. The quantity of gestures and the ability to integrate gesture with eye gaze are related to adaptive behavior.

Clinical Trial Registration:www.ClinicalTrials.gov, identifier ChiCTR1800019679.
Adolescents’ Popularity-Motivated Aggression and Prosocial Behaviors: The Roles of Callous-Unemotional Traits and Social Status Insecurity
Authors: Wright Michelle F. , Wachs Sebastian , Huang Zheng
Published: January 2021

As competition over peer status becomes intense during adolescence, some adolescents develop insecure feelings regarding their social standing among their peers (i.e., social status insecurity). These adolescents sometimes use aggression to defend or promote their status. The aim of this study was to examine the relationships among social status insecurity, callous-unemotional (CU) traits, and popularity-motivated aggression and prosocial behaviors among adolescents, while controlling for gender. Another purpose was to examine the potential moderating role of CU traits in these relationships. Participants were 1,047 (49.2% girls; Mage = 12.44 years; age range from 11 to 14 years) in the 7th or 8th grades from a large Midwestern city. They completed questionnaires on social status insecurity, CU traits, and popularity-motivated relational aggression, physical aggression, cyberaggression, and prosocial behaviors. A structural regression model was conducted, with gender as a covariate. The model had adequate fit. Social status insecurity was associated positively with callousness, unemotional, and popularity-motivated aggression and related negatively to popularity-motivated prosocial behaviors. High social status insecurity was related to greater popularity-motivated aggression when adolescents had high callousness traits. The findings have implications for understanding the individual characteristics associated with social status insecurity.
The Sense of Self Over Time: Assessing Diachronicity in Dissociative Identity Disorder, Psychosis and Healthy Comparison Groups
Authors: Dorahy Martin J. , Huntjens Rafaële J. C. , Marsh Rosemary J. , Johnson Brooke , Fox Kate , Middleton Warwick
Published: January 2021

Dissociative experiences have been associated with diachronic disunity. Yet, this work is in its infancy. Dissociative identity disorder (DID) is characterized by different identity states reporting their own relatively continuous sense of self. The degree to which patients in dissociative identity states experience diachronic unity (i.e., sense of self over time) has not been empirically explored. This study examined the degree to which patients in dissociative identity states experienced diachronic unity. Participants were DID adults (n=14) assessed in adult and child identity states, adults with a psychotic illness (n=19), adults from the general population (n=55), children from the general population (n=26) and adults imagining themselves as children (n=23). They completed the Diachronic Disunity Scale (DDS), the Dissociative Experiences Scale (DES), and the Self-Concept Clarity Scale (SCCS). Diachronic disunity was not limited to psychiatric groups, but evident to some degree in all adult and child samples. The DID adult sample experienced more dissociation and self-confusion than the psychosis and adult comparison groups, but did not differ on the diachronic measure. DID patients in their child identity states and child comparisons showed disunity and were significantly different from child simulators, who showed relatively more unity. Results suggest that DID patients in either adult or child dissociative identity states, like those in other samples, do not universally experience themselves as having a consistent sense of self over time.
By the Book or Out of the Box? Top Decision Maker Cognitive Style, Gender, and Firm Absorptive Capacity
Authors: Pryor Christopher , Hirth Robert , Jin Yanghua
Published: January 2021

Despite scholars’ early emphasis on the role people play in fostering firms’ absorptive capacity (AC), research has not deeply explored the individual-level antecedents of this important capability. We draw on adaptive-innovative theory to explain how top decision makers’ cognitive styles can influence the degree to which their firms develop AC. Top decision makers who have high adaptive cognitive style prefer to adhere to existing norms, follow established procedures, and rely on current knowledge, and we argue that these attributes will strengthen those dimensions of AC based on firms’ existing knowledge and knowledge-assimilation abilities. Top decision makers who have high innovative cognitive style are more likely to reframe information, experiment with new problem-solving approaches, and take risks by violating norms, and we argue that these attributes may strengthen those dimensions of AC based on firms’ acquisition of new knowledge and the assimilation of knowledge throughout the firm. We also argue that gender differences may moderate these effects. Empirical results support our hypotheses.
Defensive Functioning Moderates the Effects of Nondirective Meditation
Authors: Hersoug Anne Grete , Wærsted Morten , Lau Bjørn
Published: January 2021

We have recently found that nondirective meditation facilitates stress reduction. This supplementary study investigated whether defensive functioning would moderate these beneficial effects. We explored the occurrence of defense mechanisms and the impact of defensive functioning on the outcome of companies’ stress management programs regarding worries nervousness, mental distress, sleep problems, and muscle pain. The sample was a population of active, working professionals recruited from Norwegian companies (n = 105). The intervention group obtained significant benefits on all outcome measures, but there were no effects in the control group. We analyzed defensive functioning with the self-report questionnaire, Life Style Index, at four time points. The healthy adults who participated had a low level of defense scores at the outset. There was a significant reduction in the level of defenses in both groups over the study period, 6 months. Defensive functioning significantly moderated the change of the outcome measures from baseline to follow-up in the intervention group, but not in the control group.
Evidence for Specific Associations Between Depressive Symptoms, Psychotic Experiences, and Suicidal Ideation in Chilean Adolescents From the General Population
Authors: Núñez Daniel , Monjes Pía , Campos Susana , Wigman Johanna T. W.
Published: January 2021

Associations between psychotic experiences and suicidal ideation are not yet fully understood, and the potential role of depressive symptoms in this relationship remains unclear. The current study examined relationships between depressive symptoms (DS), psychotic experiences (PE) and suicidal ideation (SI) using two complementary approaches on cross-sectional data from a community sample of adolescents aged 13–19 years (N = 1,591). First, we investigated the association between the three domains using mediation analysis, showing that depressive symptoms partly mediate the relationship between psychotic experiences and suicidal ideation. Second, we looked at associations between the three domains at item level using network analysis. Specific associations between symptoms of the three domains were found, indicating depressive symptoms of sadness, avolition, pessimism, and self-criticalness/worthlessness as the most central symptoms in the network. Suicidal ideation was associated with the depressive symptoms pessimism and worthlessness, to social anxiety, and to perceptual anomalies. Our results show that the mediating effect of depressive symptoms between psychotic experiences and suicidal ideation may be due to associations between specific aspects of SI, depressive symptoms and psychotic experiences. These findings can contribute to the planning of health services and programs aimed at the timely detection of psychopathology and suicidal risk in young people.
Plasma and Magnetic Field Turbulence in the Earth’s Magnetosheath at Ion Scales
Authors: Rakhmanova Liudmila , Riazantseva Maria , Zastenker Georgy
Published: January 2021

Crossing the Earth’s bow shock is known to crucially affect solar wind plasma including changes in turbulent cascade. The present review summarizes results of more than 15 years of experimental exploration into magnetosheath turbulence. Great contributions to understanding turbulence development inside the magnetosheath was made by means of recent multi-spacecraft missions. We introduce the main results provided by them together with first observations of the turbulent cascade based on direct plasma measurements by the Spektr-R spacecraft in the magnetosheath. Recent results on solar wind effects on turbulence in the magnetosheath are also discussed.
Reducing Antimicrobial Usage in Small-Scale Chicken Farms in Vietnam: A 3-Year Intervention Study
Authors: Phu Doan Hoang , Cuong Nguyen Van , Truong Dinh Bao , Kiet Bach Tuan , Hien Vo Be , Thu Ho Thi Viet , Yen Lam Kim , Minh Nguyen Thi Tuyet , Padungtod Pawin , Setyawan Erry , Thwaites Guy , Rushton Jonathan , Carrique-Mas Juan
Published: January 2021

Indiscriminate antimicrobial use (AMU) in animal production is a driver of antimicrobial resistance globally. There is a need to define sustainable interventions to reduce AMU in small-scale production systems, which currently represent the most widespread farming systems in South East Asia and many low- and middle-income countries. We conducted a before-and-after intervention study on a random sample of small-scale chicken farms in the Mekong Delta of Vietnam from 2016 to 2019. The study included a baseline followed by an intervention phase where farmers were provided with regular veterinary advice on flock health and husbandry, as well as antimicrobial replacement products. Of 102 recruited farms (raising >100 chickens per flock cycle), thirty-five (34.2%) entered the intervention phase, whilst the rest stopped raising chickens, mainly due to suboptimal flock performance. Through the implementation of our intervention, chicken flocks reduced levels of AMU by 66% [adjusted hazard ratio (HR) = 0.34; p = 0.002) from a baseline of 343.4 Animal Daily Doses per 1,000 chicken-days and decreased weekly mortality by 40% (adjusted HR = 0.60; p = 0.005) from a baseline mortality of 1.60 per 100 birds. Chicken bodyweight increased by 100 g (p = 0.002) in intervention flocks. Our findings demonstrate that the provision of veterinary advice can achieve substantial reductions in AMU in small-scale production systems without compromising flock health and productivity.
Diagnostic Sequences That Distinguish M. avium Subspecies Strains
Authors: Bannantine John P. , Stabel Judith R. , Bayles Darrell O. , Conde Cyril , Biet Franck
Published: January 2021

Over a decade ago Mycobacterium avium subspecies paratuberculosis (Map) specific genes were initially identified in a whole genome context by comparing draft genome sequences of Map strain K-10 with Mycobacterium avium subspecies hominissuis (Mah) strain 104. This resulted in identification of 32 Map specific genes, not including repetitive elements, based on the two-genome comparison. The goal of this study was to define a more complete catalog of M. avium subspecies-specific genes. This is important for obtaining additional diagnostic targets for Johne's disease detection and for understanding the unique biology, evolution and niche adaptation of these organisms. There are now over 28 complete genome sequences representing three M. avium subspecies, including avium (Maa), Mah, and Map. We have conducted a comprehensive comparison of these genomes using two independent pan genomic comparison tools, PanOCT and Roary. This has led to the identification of more than 250 subspecies defining genes common to both analyses. The majority of these genes are arranged in clusters called genomic islands. We further reduced the number of diagnostic targets by excluding sequences having high BLAST similarity to other mycobacterial species recently added to the National Center for Biotechnology Information database. Genes identified as diagnostic following these bioinformatic approaches were further tested by DNA amplification PCR on an additional 20 M. avium subspecies strains. This combined approach confirmed 86 genes as Map-specific, seven as Maa-specific and three as Mah-specific. A single-tube PCR reaction was conducted as a proof of concept method to quickly distinguish M. avium subspecies strains. With these novel data, researchers can classify isolates in their freezers, quickly characterize clinical samples, and functionally analyze these unique genes.
A Survey of Advances in Landscape Analysis for Optimisation
Authors: Malan Katherine Mary
Published: January 2021

Fitness landscapes were proposed in 1932 as an abstract notion for understanding biological evolution and were later used to explain evolutionary algorithm behaviour. The last ten years has seen the field of fitness landscape analysis develop from a largely theoretical idea in evolutionary computation to a practical tool applied in optimisation in general and more recently in machine learning. With this widened scope, new types of landscapes have emerged such as multiobjective landscapes, violation landscapes, dynamic and coupled landscapes and error landscapes. This survey is a follow-up from a 2013 survey on fitness landscapes and includes an additional 11 landscape analysis techniques. The paper also includes a survey on the applications of landscape analysis for understanding complex problems and explaining algorithm behaviour, as well as algorithm performance prediction and automated algorithm configuration and selection. The extensive use of landscape analysis in a broad range of areas highlights the wide applicability of the techniques and the paper discusses some opportunities for further research in this growing field.
Analysis of Risk-Based Operational Bird Strike Prevention
Authors: Metz Isabel C. , Ellerbroek Joost , Mühlhausen Thorsten , Kügler Dirk , Hoekstra Jacco M.
Published: January 2021

Bird strike prevention in civil aviation has traditionally focused on the airport perimeter. Since the risk of especially damaging bird strikes outside the airport boundaries is rising, this paper investigates the safety potential of operational bird strike prevention involving pilots and controllers. In such a concept, controllers would be equipped with a bird strike advisory system, allowing them to delay departures which are most vulnerable to the consequences of bird strikes in case of high bird strike risk. An initial study has shown the strong potential of the concept to prevent bird strikes in case of perfect bird movement prediction. This paper takes the research to the next level by taking into account the limited predictability of bird tracks. As such, the collision avoidance algorithm is extended to a bird strike risk algorithm. The risk of bird strikes is calculated for birds expected to cross the extended runway center line and to cause aircraft damage upon impact. By specifically targeting these birds and excluding birds lingering on the runway which are taken care of by the local wildlife control, capacity reductions should be limited, and the implementation remain feasible. The extrapolation of bird tracks is performed by simple linear regression based on the bird positions known at the intended take-off times. To calculate the probability of collision, uncertainties resulting from variability in bird velocity and track are included. The study demonstrates the necessity to limit alerts to potentially damaging strikes with birds crossing the extended runway center line to keep the imposed delays tolerable for airports operating at their capacity limits. It is shown that predicting bird movements based on simple linear regression without considering individual bird behavior is insufficient to achieve a safety-effect. Hence, in-depth studies of multi-year bird data to develop bird behavior models and reliable predictions are recommended for future research. This is expected to facilitate the implementation of a bird strike advisory system satisfying both safety and capacity aspects.
Effect of Dietary Anthocyanin-Extracted Residue on Meat Oxidation and Fatty Acid Profile of Male Dairy Cattle
Authors: Prommachart Ronnachai , Cherdthong Anusorn , Navanukraw Chainarong , Pongdontri Paweena , Taron Wichit , Uriyapongson Juntanee , Uriyapongson Suthipong
Published: January 2021

This research aimed to evaluate the effects of anthocyanin-extracted residue (AER) in the diet of cattle on meat oxidation during storage and on the fatty acid profiles of the meat. Sixteen male dairy cattle (average body weight 160 ± 10.6 kg) were allotted to feed in a completely randomized design (CRD) with four levels of AER supplementation, 0, 20, 40, and 60 g/kg dry matter (DM) in the total mixed ration (TMR). These TMR diets were fed ad libitum to the cattle throughout the trial. At the end of the feeding trial (125 days), all cattle were slaughtered and meat samples from the Longissimus dorsi (LD) muscle were collected to assess meat oxidation and fatty acid profile. The antioxidant effect of AER on meat oxidation was investigated during 14 days of storage based on color, myoglobin redox forms, lipid, and protein oxidation. The results showed meat from cattle fed AER had better color stability, lower oxidation of lipid, protein and myoglobin than did meat from cattle fed the control diet (0 g/kg AER). Furthermore, fatty acid profiles were affected by AER supplementation with an increase in the concentration of n-3 polyunsaturated fatty acids (PUFA). These results support the inclusion of AER supplementation as a natural antioxidant in cattle to reduce meat oxidation and increase PUFA in meat.
UHPLC-ESI-OT-MS Phenolics Profiling, Free Radical Scavenging, Antibacterial and Nematicidal Activities of “Yellow-Brown Resins” from Larrea spp.
Authors: Gómez Jessica , Simirgiotis Mario J. , Manrique Sofía , Piñeiro Mauricio , Lima Beatriz , Bórquez Jorge , Feresin Gabriela E. , Tapia Alejandro
Published: January 2021

This research was designed to investigate the metabolite profiling, phenolics and flavonoids content and the potential antioxidant, antibacterial and nematicidal activities of “yellow-brown resins” from Larrea divaricata Cav (LdRe) and L. nitida Cav (LnRe). Metabolite profiling was obtained using an ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP) and lipid peroxidation in erythrocytes (LP). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. In addition, the potential combinatory effect was analyzed with the fractional inhibitory concentration index (FICI) values using the checkerboard design. The nematicidal activity was carried out according to a standardized protocol. LdRe and LnRe showed a strong capture of the DPPH radical withvalues around 8.4 µg resin/mL; FRAP (1.69–1.94 mgTE/ g resin), TEAC (1.08–1.09 mgTE/g resin) and LP (81–82% at 100 µg of resin/mL) assays. A strong antimicrobial activity was displayed by both resins against methicillin-sensitive Staphylococcus aureus ATCC 25923(MSSA) and methicillin-resistant S. aureus ATCC 43300(MRSA) (MICs = 16–32 µg resin/mL). Additionally, the combination of LdRe or LnRe with the antibiotic cefotaxime showed an indifferent effect (FICI values = 1–1.25), however, this combinationcould be a potential strategy to reduce the drug doses, and in this way can be a potential alternative to reduce bacterial resistance. On the other hand, the resins showed a scarce nematicidal potential toward J2 Meloidogyne incognita; an important nematode infecting horticultural crops. Phenolics compounds were identified by UHPLC-PDA-OT-MS analysis, updating the knowledge on the chemical profile of these species. These results, together with the high content of quantified phenolics and flavonoids, allow the phenolics-enriched resins of these two Larrea species to be considered as a promising sustainable source of compounds of pharmacological interest.
Redox Homeostasis in Poultry: Regulatory Roles of NF-κB
Authors: Surai Peter F. , Kochish Ivan I. , Kidd Michael T.
Published: January 2021

Redox biology is a very quickly developing area of modern biological sciences, and roles of redox homeostasis in health and disease have recently received tremendous attention. There are a range of redox pairs in the cells/tissues responsible for redox homeostasis maintenance/regulation. In general, all redox elements are interconnected and regulated by various means, including antioxidant and vitagene networks. The redox status is responsible for maintenance of cell signaling and cell stress adaptation. Physiological roles of redox homeostasis maintenance in avian species, including poultry, have received limited attention and are poorly characterized. However, for the last 5 years, this topic attracted much attention, and a range of publications covered some related aspects. In fact, transcription factor Nrf2 was shown to be a master regulator of antioxidant defenses via activation of various vitagenes and other protective molecules to maintain redox homeostasis in cells/tissues. It was shown that Nrf2 is closely related to another transcription factor, namely, NF-κB, responsible for control of inflammation; however, its roles in poultry have not yet been characterized. Therefore, the aim of this review is to describe a current view on NF-κB functioning in poultry with a specific emphasis to its nutritional modulation under various stress conditions. In particular, on the one hand, it has been shown that, in many stress conditions in poultry, NF-κB activation can lead to increased synthesis of proinflammatory cytokines leading to systemic inflammation. On the other hand, there are a range of nutrients/supplements that can downregulate NF-κB and decrease the negative consequences of stress-related disturbances in redox homeostasis. In general, vitagene–NF-κB interactions in relation to redox balance homeostasis, immunity, and gut health in poultry production await further research.
Antioxidants and Therapeutic Targets in Ovarian Clear Cell Carcinoma
Authors: Amano Tsukuru , Murakami Atsushi , Murakami Takashi , Chano Tokuhiro
Published: January 2021

Ovarian clear cell carcinomas (OCCCs) are resistant to conventional anti-cancer drugs; moreover, the prognoses of advanced or recurrent patients are extremely poor. OCCCs often arise from endometriosis associated with strong oxidative stress. Of note, the stress involved in OCCCs can be divided into the following two categories: (a) carcinogenesis from endometriosis to OCCC and (b) factors related to treatment after carcinogenesis. Antioxidants can reduce the risk of OCCC formation by quenching reactive oxygen species (ROS); however, the oxidant stress-tolerant properties assist in the survival of OCCC cells when the malignant transformation has already occurred. Moreover, the acquisition of oxidative stress resistance is also involved in the cancer stemness of OCCC. This review summarizes the recent advances in the process and prevention of carcinogenesis, the characteristic nature of tumors, and the treatment of post-refractory OCCCs, which are highly linked to oxidative stress. Although therapeutic approaches should still be improved against OCCCs, multi-combinatorial treatments including nucleic acid-based drugs directed to the transcriptional profile of each OCCC are expected to improve the outcomes of patients.
The Interactions between Polyphenols and Microorganisms, Especially Gut Microbiota
Authors: Makarewicz Małgorzata , Drożdż Iwona , Tarko Tomasz , Duda-Chodak Aleksandra
Published: January 2021

This review presents the comprehensive knowledge about the bidirectional relationship between polyphenols and the gut microbiome. The first part is related to polyphenols’ impacts on various microorganisms, especially bacteria, and their influence on intestinal pathogens. The research data on the mechanisms of polyphenol action were collected together and organized. The impact of various polyphenols groups on intestinal bacteria both on the whole “microbiota” and on particular species, including probiotics, are presented. Moreover, the impact of polyphenols present in food (bound to the matrix) was compared with the purified polyphenols (such as in dietary supplements) as well as polyphenols in the form of derivatives (such as glycosides) with those in the form of aglycones. The second part of the paper discusses in detail the mechanisms (pathways) and the role of bacterial biotransformation of the most important groups of polyphenols, including the production of bioactive metabolites with a significant impact on the human organism (both positive and negative).
On the Thermal Stresses Due to Weathering in Natural Stones
Authors: Ito William Hideki , Scussiato Talita , Vagnon Federico , Ferrero Anna Maria , Migliazza Maria Rita , Ramis Jacqueline , de Queiroz Paulo Ivo Braga
Published: January 2021

Natural weathering is known as one of the key mechanisms causing degradation in building materials. Great efforts have been made to develop new materials and new processes for protecting those that already exist. Natural stones are an example of a natural material that has been extensively used for building construction since ancient times. In addition, they fit durability, aesthetic, and mechanical requirements. Thus, they still have great importance in the construction business nowadays. Though chemical interactions in natural stones, such as oxidation or hydrolyses, have been widely studied, in the last few decades, the physical weathering due to daily temperature variations has begun to be considered as a key mechanism of degradation and has been incorporated in international standards. This process is particularly important in calcitic marble slabs, where it can cause extensive damages to facades. Consequently, there are restrictive rules for the use of marble as an external coating material in many countries. In this paper, the thermal stresses induced by daily variations in temperature are calculated using geographic and meteorological information. The concept of sol-air temperature is used to estimate the temperatures of the hidden and exposed surfaces of a slab, and Fourier’s law and the theory of elasticity are used to calculate the temperature and stress distribution, respectively. The proposed methodology allows for a detailed reconstruction of the stress induced inside marble slabs using parameters commonly acquired in meteorological stations as input data. The developed methodology was validated by comparing in-situ measurements of the temperature of a building in Pescara (Central Italy). A good correlation between the theoretical and real temperatures was found; in particular, the peak tensile stresses inside the slabs were estimated at 75 kPa.
Experiences with Establishing a Simulation Scenario of the City of Duisburg with Real Traffic Volume
Authors: Ma Xiaoyi , Hu Xiaowei , Weber Thomas , Schramm Dieter
Published: January 2021

This article presents the experience of building a simulation scenario of the whole city of Duisburg using real traffic data. The establishment of the simulation scenario is based on road network and traffic volume. In most cases, it is hard to collect all data sources with high precision. Moreover, it is time-consuming to set up a realistic traffic scenario. Even with available data, conversion, calibration, and validation all take a large effort. With the increase of the respective simulation area, the difficulty and workload rise. In this study, a simulation scenario of the whole city of Duisburg with the road network area of 232 km2 and Origin/Destination (OD) matrix area over 800 km2 was established in the software package SUMO. Four cases with different networks and traffic volumes were built and compared with real traffic data collected from induction loops. The percentage of simulated traffic volume in real traffic volume range can be up to 72.22%.
Hair-Growth-Promoting Effects of Fermented Red Ginseng Marc and Traditional Polyherb Formula in C57BL/6 Mice
Authors: Song Phil Hyun , Park Gyu-Ryeul , Kim Yoon-Hae , Jung Dae Hwa , Ku Sae-Kwang , Song Chang-Hyun
Published: January 2021

An abnormal hair-growth cycle induces hair loss, which affects psychological distress and impairs life quality. Red ginseng marc (RGM) is usually discarded as a byproduct after extracting red ginseng, but several studies have shown that the RGM still has bioactive components including ginsenosides. Therefore, the hair-growth effects of fermented RGM (fRGM) and traditional polyherb formula (PH) were examined in C57BL/6 mice. The dorsal hairs of mice were depilated, and they were topically treated with fRGM or PH at 400, 200 and 100 mg/kg or the combination of both middle doses (combi) once a day for two weeks. The hair-covering regions were significantly increased with higher doses of fRGM and PH and in combi groups, compared with the control treated with distilled water. Hair length, thickness and weight also increased in the treatment groups. In particular, the fRGM and PH increased the anagen-phased hair follicles, the follicular diameters and the dermal thickness. Immunostains for Ki-67 showed the anagen-phased cell division in the treatment groups. The beneficial effects were greater in the high doses of fRGM and PH and the combi groups. These suggest hair-growth-promoting effects of fRGM, PH and the combination by enhancing the hair-growth cycle.
Analytical Pyrolysis of the Fungal Melanins from Ochroconis spp. Isolated from Lascaux Cave, France
Authors: Saiz-Jimenez Cesareo , Martin-Sanchez Pedro M. , Gonzalez-Perez Jose A. , Hermosin Bernardo
Published: January 2021

Analytical pyrolysis is a tool widely used for investigating the nature of macromolecules. This technique has been applied with success for solving the chemical structure of geo- and biopolymers. However, with complex macromolecules, such as fungal melanins, analytical pyrolysis presents some drawbacks due to the fact that they rarely can be obtained in a pure grade and contains a mixture of polysaccharides, chitin, proteins, nucleic acids, and lipids, among other materials. Analytical pyrolysis permits the characterization of the compounds co-extracted or linked to the melanin. Nevertheless, our data revealed that under the conditions usually employed in the pyrolysis of melanins, the technique presented serious limitations due to the complex structure and diversity of components that suffer extensive thermal degradation through secondary reactions with the production of considerable number of artifacts. To the light of pyrolysis data, the melanin of Ochroconis spp. is not based on 3,4-dihydroxyphenylalanine (DOPA) or 1,8-dihydroxynaphthalene (DHN), and it is suggested that it could be a type of pyomelanin, based on the polymerization of homogentisic acid and other phenolic compounds.
Review and New Aspects in Combining Multipoint Moulding and Additive Manufacturing
Authors: Herzog Thomas , Tille Carsten
Published: January 2021

Additive manufacturing has become a very important manufacturing method in the last years. With additive manufacturing, a higher level of function integration can be achieved compared to traditional manufacturing technologies. However, the manufacturing of larger parts leads to long construction times. A possible solution is the combination of multipoint moulding with additive manufactured form elements. This article reviews the state of technology for multipoint moulding and additive manufacturing. Moreover, the state of technology is analysed to outline the possibilities and challenges of combining both technologies. The review shows that there has been research on different challenges of the new production process. On the other hand, it turns out clearly that there are many open points at the intersections of both technologies. Finally, the areas where further research is necessary are described in detail.
Optimization of the Acetification Stage in the Production of Wine Vinegar by Use of Two Serial Bioreactors
Authors: Álvarez-Cáliz Carmen M. , Santos-Dueñas Inés María , Jiménez-Hornero Jorge E. , García-García Isidoro
Published: January 2021

In the scope of a broader study about wine acetification, previous works concluded that using a single bioreactor hindered simultaneously reaching high productivities with high substrate consumption and the use of two serially arranged bioreactors (TSAB) could achieve such goal. Then, the aim of this work is the optimization, using Karush–Kuhn–Tucker (KKT) conditions, of this TSAB using polynomial models previously obtained. The ranges for the operational variables leading to either maximum and minimum mean rate of acetification of 0.11 ≤ (rA)global ≤ 0.27 g acetic acid·(100 mL·h)−1 and acetic acid production of 14.7 ≤ Pm ≤ 36.6 g acetic acid·h−1 were identified; the results show that simultaneously maximizing (rA)global and Pm is not possible so, depending on the specific objective, different operational ranges must be used. Additionally, it is possible to reach a productivity close to the maximum one (34.6 ≤ Pm ≤ 35.5 g acetic acid·h−1) with an almost complete substrate use [0.2% ≤ Eu2 ≤ 1.5% (v/v)]. Finally, comparing the performance of the bioreactors operating in series and in parallel revealed that the former choice resulted in greater production.
Individual Alpha Peak Frequency, an Important Biomarker for Live Z-Score Training Neurofeedback in Adolescents with Learning Disabilities
Authors: Pérez-Elvira Rubén , Oltra-Cucarella Javier , Carrobles José Antonio , Teodoru Minodora , Bacila Ciprian , Neamtu Bogdan
Published: January 2021

Learning disabilities (LDs) have an estimated prevalence between 5% and 9% in the pediatric population and are associated with difficulties in reading, arithmetic, and writing. Previous electroencephalography (EEG) research has reported a lag in alpha-band development in specific LD phenotypes, which seems to offer a possible explanation for differences in EEG maturation. In this study, 40 adolescents aged 10–15 years with LDs underwent 10 sessions of Live Z-Score Training Neurofeedback (LZT-NF) Training to improve their cognition and behavior. Based on the individual alpha peak frequency (i-APF) values from the spectrogram, a group with normal i-APF (ni-APF) and a group with low i-APF (li-APF) were compared in a pre-and-post-LZT-NF intervention. There were no statistical differences in age, gender, or the distribution of LDs between the groups. The li-APF group showed a higher theta absolute power in P4 (p = 0.016) at baseline and higher Hi-Beta absolute power in F3 (p = 0.007) post-treatment compared with the ni-APF group. In both groups, extreme waves (absolute Z-score of ≥1.5) were more likely to move toward the normative values, with better results in the ni-APF group. Conversely, the waves within the normal range at baseline were more likely to move out of the range after treatment in the li-APF group. Our results provide evidence of a viable biomarker for identifying optimal responders for the LZT-NF technique based on the i-APF metric reflecting the patient’s neurophysiological individuality.