bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan Zachman Framework Dalam Perancangan Sistem Informasi Manajemen Keuangan Sekolah
Authors: Bahri Saeful
Published: October 2021

Sekolah swasta tidak terlepas dari sumber pendanaan dari siswa maupun dari bantuan operasional sekolah, pendanaan yang diterima sekolah tentunya memiliki kegunaan yang berbeda- beda sesuai dengan peruntukan dana tersebut dibayarkan oleh siswa maupun dari bantuan operasional sekolah, sehingga dalam proses pencatatan dan pengelolaan dana tersebut diperlukan ketelitian supaya tidak terjadinya kesalahan dalam proses pembuatan laporan keuangan disetiap akhir periode. sehingga sekolah memerlukan suatu sistem dalam pengelolaan hal tersebut. Kemajuan zaman yang semakin modern menjadikan suatu sistem aplikasi otomasi dan terintegrasi tidak hanya sebagai nilai tambah tetapi menjadi sebuah kebutuhan dalam menjalankan suatu organisasi. Menanggapi hal ini peneliti mencoba menggambarkan suatu rancangan sistem informasi manajemen keuangan yang bisa digunakan sesuai dengan keperluan sekolah dengan menggunakan kerangka kerja arsitektur enterprise yaitu zachman framework yang bisa membantu dalam proses pembuatan sistem keuangan sekolah ini, rancangan sistem atau blueprint sistem ini mengedepankan pandangan dari berbagai persektif yang ada di sekolah dan dijadikan sebagai acuan dalam pembuatan sistem ini sehingga sistem bisa dirancang sesuai dengan kebutuhan dan masalah yang di hadapi sekolah khususnya dalam pengelolaan keuangannya, dalam pengambilan data yang dibutuhkan peneliti melakukan observasi dan wawancara langsung ke lokasi penelitian, yaitu SDIT Andalusia dan dalam pengelolaan keuangan masih terdapat beberapa kesalahan seperti redudansi data, kesalahan penulisan dan pencatatan dikarenakan masih menggunakan pencatatan manual, sehingga dalam penyusunan laporan keuangan sering terjadi keslahan jika tidak teliti dalam melakukan penyusunan tersebut. Dalam penelitian ini penulis hanya memuat empat persfektif yang ada dalam kerangka zachman diantaranya yaitu: perspektif planner, perspektif owner, perspektif designer dan perspektif builder. Hasil dari penelitian ini berupa suatu rancangan suatu sistem informasi keuangan yang dapat digunakan sebagai acuan dalam pembuatan suatu aplikasi sistem informasi keuangan yang cocok digunakan oleh sekolah, sehingga dikemudian hari bisa digunakan sebagai sarana untuk mempermudah dan mengatasi kesalahan kesalahan yang sering terjadi dalam proses pencatatan keuangan dan pencatatan laporan keuangan di sekolah
Pengembangan dan Pengujian Aplikasi Website Career Center ITERA
Authors: Wahyuji Agis Tri
Published: October 2021

Seiring dengan berjalannya waktu, Institut Teknologi Sumatera (ITERA) telah meluluskan lulusan dengan berbagai keahlian yang dapat bersaing dengan para lulusan-lulusan dari perguruan tinggi lain. Tetapi hingga saat ini Insitut Teknologi Sumatera (ITERA) belum memiliki sistem yang dapat memberikan informasi lowongan pekerjaan dan pengembangan karir bagi alumni yang baru lulus sehingga menyebabkan para lulusan berusaha sendiri dalam menemukan dan mencari lowongan pekerjaan, dan juga membutuhkan waktu dalam mendapatkan pekerjaan impian yang mengakibatkan alumni fresh graduate mengangur dalam jangka waktu yang cukup lama. ITERA telah memfasilitasi alumni fresh graduate untuk mendapatkan pekerjaan dengan mengadakan job fair, akan tetapi dirasa belum efektif dikarenakan lulusan yang ikut serta didalamnya sedikit, yaitu hanya 40% dari total lulusan yang berstatus fresh graduate. Dalam pengembangan dan pengujian aplikasi ini, menggunakan metodologi pengembangan System Development Life Cycle (SDLC) dengan mengacu pada model waterfall serta UML sebagai toolsnya. Sedangkan bahasa pemrograman yang digunakan adalah Framework Codeigniter, Bootstrap, dan Xampp sebagai server local dan pengujian aplikasi ini menggunakan teknik Black-Box dengan metode equivalence partititoning dan menggunakan teknik White-Box dengan Katalon Studio untuk toolsnya. Pengujian dilakukan secara localhost. Hasil pengujian menunjukkan bahwa pengembangan website career center ini menunjukan bahwa aplikasi dapat berjalan dengan baik sesuai dengan yang diharapkan dalam memberikan informasi mengenai lowongan kerja
Sistem Informasi Penjualan Kredit Barang Berbasis Vb.Net Pada Pd Ria Mulya Karawang
Authors: Yusuf Arif Maulana , Indaryono Indaryono , Sari Juwita Novita
Published: October 2021

Aktifitas penjualan kredit barang pada Pd Ria Mulya Karawang merupakan salah satu proses perusahaan yang bergerak dibidang penjualan peralatan rumah tangga. Dalam hal penjualan kredit dan penjualan cash. Untuk meningkatkan dana perusahaan, diberlakukan kepada setiap customer yang melakukan pembelian secara kredit dikenakan bunga yang diharapkan dalam kurun waktu beberapa bulan, dana-dana yang diperoleh dari bunga ini dapat memperbesar pendapatan perusahaan. Tujuan dari penelitian ini adalah untuk mengetahui sistem penjualan kredit barang yang sedang berjalan pada PD Ria Mulia Karawang seperti apa. Metodologi yang digunakan dalam penelitian ini meliputi Analisa Sistem, Perancangan Sistem, dan Implementasi Sistem. Analisa Sistem dalam bahasa, hal ini meliputi Analisa Sistem yang Berjalan dan Analisa Masalah serta Solusi. Perancangan Sistem meliputi Perancangan Masukan, Keluaran, Proses dan Database. Metode implementasi sistem meliputi penyusunan program dan tes program. Dalam penyelenggaraan penjualan kredit barang ini secara administratif belum sepenuhnya dilaksanakan dengan baik. Seperti aktivitas pencatatan data penjualan kreditnya ini masih ditemukan adanya kendala seperti pembuatan laporan penjualannya dilakukan dengan merekap data pada buku penjualan. Berdasarkan permasalan tersebut diatas, penulis berpendapat bahwa untuk mengatasi permasalahan yang terjadi tersebut, perlu dirancang dan diimplementasikan suatu sistem yang terintegrasi antar fungsi-fungsi yang terkait dalam perusahaan, dengan menggunakan microsoft visual basic.net 2008 dan SQL Server 2008 sebagai databasenya. Dengan menerapan sistem ini diharapkan akan dapat bermanfaat bagi perusahaan dalam operasionalnya dimasa yang akan datang.
PENERAPAN METODE PIECES DALAM PENGEMBANGAN SISTEM INFORMASI MANAJEMEN TUGAS AKHIR (STUDI KASUS : PRODI SISTEM INFORMASI UNIYAP)
Authors: Robo Salahudin , Alam Sitti Nur , Julianto Teguh Dwi
Published: October 2021

Information Systems study program is one of the study program at the Yapis University of Papua. One of its academic activities, the processing of the Final Project, has not utilized technology optimally which the collection of final project documents are still in print out. This becomes problems faced by study program administrations such as limited storage space, damage and loss of documents, besides that the costs incurred by students are quite large for printing documents. The purpose of this study is to create an integrated final project management system so that it can facilitate the work of all parties that are directly related to student final project management. The analysis method used in this research is the PIECES method, the UML method as the system design, the waterfall as the development method and the Black box testing method used as testing method. For the data collection process, this research uses interview, observation and literature study techniques. The result of this research is a Final Project Management Information System which aims to make easier for students in managing their Final Projects and for facilitate the study program in managing student Final Projects
DESAIN GAMIFIKASI ADAPTIF UNTUK LEARNING MANAGEMENT SYSTEM MENGGUNAKAN GAMING ACHIEVEMENT GOAL
Authors: Firdaus Lukmannul Hakim
Published: October 2021

Penerapan gamifikasi pada Learning Management System (LMS) tidak hanya digunakan untuk membuat pembelajaran menjadi menyenangkan, tetapi juga harus mampu mengubah perilaku, motivasi dan hasil belajar siswa. Oleh karena itu, perlu diketahui orientasi siswa dalam belajar, agar penerapan unsur gamifikasi dapat disesuaikan dengan tujuan belajar siswa. Setiap siswa memiliki motivasi dan tujuan belajar yang beragam. Banyak penelitian sebelumnya yang telah membahas metode pembelajaran adaptif dengan memanfaatkan gamifikasi. Penelitian ini bertujuan untuk membuat sebuah desain gamifikasi yang adaptif untuk pembelajaran dalam jaringan dengan tahapan: menentukan tipe siswa, menentukan elemen gamifikasi, membuat model gamifikasi dan membuat desain gamifikasi. Metode penentuan siswa pada penelitian ini menggunakan gaming achievement goal, dimana terdapat 4 tipe siswa, yaitu super-achiever, mastery-only, performance-only dan non-achiever. Untuk menentukan tipenya, siswa harus mengisi kuesioner Achievement Goal Questionnaire Revised (AGQ-R) yang telah diterjemahkan kedalam Bahasa Indonesia. Selanjutnya, metode penentuan elemen gamifikasi dimulai dengan melakukan perancangan gamifikasi berdasarkan metode Werbach dan Hunter. Elemen tersebut kemudian dipetakan berdasarkan karakteristik masing-masing tipe pada gaming achievement goal. Agar lebih memaknai penerapan elemen gamifikasi pada desain, dibuatlah model gamifikasi. Model gamifikasi inilah yang akan menjadi dasar dalam pembuatan desain gamifikasi. Terakhir, untuk menguji desain yang telah dibuat, maka desain gamifikasi tersebut diterapkan pada sistem pembelajaran tergamifikasi yang disebut G-Learning. Pengujian dilakukan untuk mengukur kualitas hasil belajar siswa serta kinerja dari fungsional sistem. Pengujian dilakukan terhadap 100 orang siswa SMK yang dibagi kedalam dua grup, yaitu 50 orang siswa yang mengguankan G-Learning dan 50 orang siswa menggunakan Moodle. Hasil pengujian menunjukkan bahwa tidak ada perbedaan yang sigifikan antara hasil belajar siswa yang menggunakan G-Learning dengan siswa yang menggunakan Moodle. Namun, sebanyak 64.58% siswa yang menggunakan G-Learning memberikan penilaian yang positif terhadap aksesibilitas sistem, kualitas desain gamifikasi dan efek gamifikasi terhadap motivasi belajar. Hasil tersebut menunjukkan desain gamifikasi adaptif yang diajukan dapat dijadikan pilihan untuk diterapkan pada LMS sebagai model pembelajaran adaptif.
Developing English Materials for Nursing Students through Students' Need Analysis
Authors: Burhan Kristian , Putra Hendra
Published: October 2021

The study aims to find out the nursing students of AKPER YPTK Solok need in developing English materials. This research is classified as mixed method research design; qualitative research and survey research. In this research, there are two instruments; questionnaire and interview guidelines. The questionnaire aims to gain and collect information from students related to English teaching and learning. Interview guideline is used to gather information from English lecturer at AKPER YPTK Solok. The results of this research show that; (1) there are some skills and English components that are needed by the nursing students of AKPER YPTK Solok, they are; speaking skill, reading skill, vocabulary mastery, and grammar mastery. (2) This research also found that nursing students of AKPER YPTK Solok want English materials, including grammar and vocabulary related to nursing field to be more focused in teaching and learning process. Based on the results, it can be concluded that in developing English materials for nursing students, the lecturers and decision maker should know what the students and work field need through students; need analysis.
Частка (частина частки) у статутному капіталі ТОВ, її оборотоздатність: проблеми теорії і практики
Authors: РЄЗНІКОВА ВІКТОРІЯ , КРАВЕЦЬ ІРИНА , ЛУКОМСЬКА ОЛЕСЯ
Published: October 2021

Товариства з обмеженою відповідальністю (ТОВ) є найбільш популярною організаційно-правовою формою ведення бізнесу в Україні. Створення цих товариств переважно як об’єднань не менше двох осіб із поділом статутного капіталу на частки на практиці породжує питання, пов’язані з переходом частки (частини частки) учасника в статутному капіталі ТОВ, зокрема через її продаж. Актуальність обраної теми зумовлює невизначеність деяких правових норм, відсутність усталеної судової практики вирішення корпоративних спорів, що призводить до виникненнязначної кількості судових спорів щодо переходу частки (частини частки) в статутному капіталі ТОВ через її продаж. Метою статті є з’ясування правової природи частки (частини частки) у статутному капіталі ТОВ; визначення моменту виникнення права власності на частку (частину частки) в статутному капіталі ТОВ у третьої особи; виокремлення ознак, які характеризують частку (частину частки) у статутному капіталі ТОВ як товар; формулювання поняття “оборотоздатність частки (частини частки) у статутному капіталі ТОВ”. Розглянуто два підходи науковців до розуміння питання, чи частка (частина частки) в статутному капіталі ТОВ, чи корпоративні права є об’єктом права власності і, відповідно, – можуть бути предметом договору купівлі-продажу, міни тощо. Проаналізовано погляди законодавця та позиції судової практики стосовно вище окресленого питання. Обґрунтовано, що саме частка (частина частки) в статутному капіталі ТОВ є об’єктом права власності і, відповідно, – може бути предметом договору купівлі-продажу, міни тощо. Встановлено, що право власності на частку (частину частки) в статутному капіталі ТОВ у третьої особи виникає з моменту укладення договору, якщо інше не встановлено домовленістю сторін. Виокремлено ознаки, які характеризують частку (частину частки) у статутному капіталі ТОВ як товар, сформульовано поняття “оборотоздатність частки (частини частки) у статутному капіталі ТОВ”. Запропоновано зміни до законодавства, що регулює порядок відчуження частки (частини частки) у статутному капіталі ТОВ з метою його вдосконалення, зокрема, до ч. 3 ст. 20, ч. 4 ст. 21 Закону України “Про товариства з обмеженою та додатковою відповідальністю ”, ч. 1 ст. 226, ч. 6 ст. 238 Господарського процесуального кодексу України.
Efek Sinergis Kombinasi Ekstrak Rimpang Kunyit (Curcuma domestica Val.) dan Ampisilin terhadap Escherichia coli secara in vitro
Authors: Kusmana Pratama Putra
Published: October 2021

Escherichia coli (E.coli) pada kondisi tertentu dapat diobati dengan ampisilin. Namun ampisilin saat ini mulai ditinggalkan karena tingginya kasus E.coli yang resisten ampisilin. Salah satu cara untuk mengatasi masalah resistensi ini adalah dengan mengkombinasi ekstrak rimpang kunyit (ERK) dan ampisilin. Tujuan penelitian ini adalah untuk mengetahui efek antimikroba dan efek sinergis kombinasi ERK (Curcuma domestica Val.) dan ampisilin terhadap E.coli secara in vitro. Penelitian ini menggunakan desain uji esksperimental laboratorik sungguhan secara in vitro dengan metode agar diffusion. Data yang diperoleh disajikan dalam bentuk tabel dan dinalisis secara deskriptif. Dari hasil penelitian didapatkan bahwa ERK (Curcuma domestica Val.) konsentrasi 165, 330, 660 µg/ml tidak didapatkan zona inhibisi terhadap E.coli. Persentasi peningkatan zona inhibisi pada MHA-ERK-ampisilin 165, 330, 660 µg/ml : 24,11%; 38,79% ; 47,39% lebih besar dibanding ampisilin tunggal. Simpulan dari penelitian ini adalah ekstrak rimpang kunyit (Curcuma domestica Val.) 165 µg/ml, 330 µg/ml, dan 660 µg/ml tidak memiliki efek antimikroba terhadap Escherichia coli secara in vitro sedangkan kombinasi ekstrak rimpang kunyit (Curcuma domestica Val.) dan ampisilin memiliki efek sinergis terhadap Escherichia coli secara in vitro.
Fish Allergy Around the World—Precise Diagnosis to Facilitate Patient Management
Authors: Kalic Tanja , Radauer Christian , Lopata Andreas L. , Breiteneder Heimo , Hafner Christine
Published: October 2021

The accurate and precise diagnosis of IgE-mediated fish allergy is one of the biggest challenges in allergy diagnostics. A wide range of fish species that belong to evolutionary distant classes are consumed globally. Moreover, each fish species may contain multiple isoforms of a given allergen that often differ in their allergenicity. Recent studies indicated that the cross-reactivity between different fish species is limited in some cases and depends on the evolutionary conservation of the involved allergens. Fish allergens belong to several protein families with different levels of stability to food processing. Additionally, different preparation methods may contribute to specific sensitization patterns to specific fish species and allergens in different geographic regions. Here, we review the challenges and opportunities for improved diagnostic approaches to fish allergy. Current diagnostic shortcomings include the absence of important region-specific fish species in commercial in vitro and in vivo tests as well as the lack of their standardization as has been recently demonstrated for skin prick test solutions. These diagnostic shortcomings may compromise patients' safety by missing some of the relevant species and yielding false negative test results. In contrast, the avoidance of all fish as a common management approach is usually not necessary as many patients may be only sensitized to specific species and allergens. Although food challenges remain the gold standard, other diagnostic approaches are investigated such as the basophil activation test. In the context of molecular allergy diagnosis, we discuss the usefulness of single allergens and raw and heated fish extracts. Recent developments such as allergen microarrays offer the possibility to simultaneously quantify serum IgE specific to multiple allergens and allergen sources. Such multiplex platforms may be used in the future to design diagnostic allergen panels covering evolutionary distant fish species and allergens relevant for particular geographic regions.
Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis
Authors: Wu Shuang , Chen Kai , Xu Tao , Ma Ke , Gao Lei , Fu Cong , Zhang Wenjuan , Jing Changbin , Ren Chunguang , Deng Min , Chen Yi , Zhou Yi , Pan Weijun , Jia Xiaoe
Published: October 2021

Vertebrate erythropoiesis involves nuclear and chromatin condensation at the early stages of terminal differentiation, which is a unique process to distinguish mature erythrocytes from erythroblasts. However, the underlying mechanisms of chromatin condensation during erythrocyte maturation remain elusive. Here, we reported a novel zebrafish mutantcas7 with erythroid maturation deficiency. Positional cloning showed that a single base mutation in tprb gene, which encodes nucleoporin translocated promoter region (Tpr), is responsible for the disrupted erythroid maturation and upregulation of erythroid genes, including ae1-globin and be1-globin. Further investigation revealed that deficient erythropoiesis in tprbcas7 mutant was independent on HIF signaling pathway. The proportion of euchromatin was significantly increased, whereas the percentage of heterochromatin was markedly decreased in tprbcas7 mutant. In addition, TPR knockdown in human K562 cells also disrupted erythroid differentiation and dramatically elevated the expression of globin genes, which suggests that the functions of TPR in erythropoiesis are highly conserved in vertebrates. Taken together, this study revealed that Tpr played vital roles in chromatin condensation and gene regulation during erythroid maturation in vertebrates.
CDKN2B-AS1 Promotes Malignancy as a Novel Prognosis-Related Molecular Marker in the Endometrial Cancer Immune Microenvironment
Authors: Yang Di , Ma Jian , Ma Xiao-Xin
Published: October 2021

The prognosis of patients with endometrial cancer (EC) is closely associated with immune cell infiltration. Although abnormal long non-coding RNA (lncRNA) expression is also linked to poor prognosis in patients with EC, the function and action mechanism of immune infiltration-related lncRNAs underlying the occurrence and development of EC remains unclear. In this study, we analyzed lncRNA expression using The Cancer Genome Atlas and clinical data and identified six lncRNAs as prognostic markers for EC, all of which are associated with the infiltration of immune cell subtypes, as illustrated by ImmLnc database and ssGSEA analysis. Real-time quantitative polymerase chain reaction showed that CDKN2B-AS1 was significantly overexpressed in EC, whereas its knockdown inhibited the proliferation and invasion of EC cells and the in vivo growth of transplanted tumors in nude mice. Finally, we constructed a competing endogenous RNA regulatory network and conducted Gene Ontology enrichment analysis to elucidate the potential molecular mechanism underlying CDKN2B-AS1 function. Overall, we identified molecular targets associated with immune infiltration and prognosis and provide new insights into the development of molecular therapies and treatment strategies against EC.
LeuO, a LysR-Type Transcriptional Regulator, Is Involved in Biofilm Formation and Virulence of Acinetobacter baumannii
Authors: Islam Md. Maidul , Kim Kyeongmin , Lee Je Chul , Shin Minsang
Published: October 2021

Acinetobacter baumannii is an important nosocomial pathogen that can survive in different environmental conditions and poses a severe threat to public health due to its multidrug resistance properties. Research on transcriptional regulators, which play an essential role in adjusting to new environments, could provide new insights into A. baumannii pathogenesis. LysR-type transcriptional regulators (LTTRs) are structurally conserved among bacterial species and regulate virulence in many pathogens. We identified a novel LTTR, designated as LeuO encoded in the A. baumannii genome. After construction of LeuO mutant strain, transcriptome analysis showed that LeuO regulates the expression of 194 upregulated genes and 108 downregulated genes responsible for various functions and our qPCR validation of several differentially expressed genes support transcriptome data. Our results demonstrated that disruption of LeuO led to increased biofilm formation and increased pathogenicity in an animal model. However, the adherence and surface motility of the LeuO mutant were reduced compared with those of the wild-type strain. We observed some mutations on amino acids sequence of LeuO in clinical isolates. These mutations in the A. baumannii biofilm regulator LeuO may cause hyper-biofilm in the tested clinical isolates. This study is the first to demonstrate the association between the LTTR member LeuO and virulence traits of A. baumannii.
Potential for Negative Emissions by Carbon Capture and Storage From a Novel Electric Plasma Calcination Process for Pulp and Paper Mills
Authors: Svensson Elin , Wiertzema Holger , Harvey Simon
Published: October 2021

The pulp and paper industry has a high potential to contribute to negative emissions through carbon capture and storage (CCS) applied to existing processes. However, there is a need to investigate how CCS solutions also can be combined with implementation of other emerging technologies in pulp and paper mills. This paper investigates the integration of a novel calcination process in two kraft mills and evaluates its potential combination with capture and storage of CO2 from the calcination plant. The alternative calcination process uses electric gas-plasma technology combined with steam slaking and allows replacing the conventional fuel-driven lime kilns with a process driven by electricity. The novel calcination process generates a pure, biogenic, CO2 stream, which provides an opportunity to achieve negative emissions at relatively lower costs. The potential reduction of greenhouse gas emissions when replacing the lime kiln with the plasma calcination concept depends strongly on the emissions intensity of grid electricity, and on whether fossil fuel or biomass was used as a fuel in the lime kiln. If fossil fuel is replaced and electricity is associated with very low emissions, avoided CO2 emissions reach ~50 kt/a for the smaller mill investigated in the paper (ca 400 kt pulp per year) and almost 100 kt/a for the larger mill (ca 700 kt pulp per year). Further emission reductions could then be achieved through CCS from the electrified calcination process, with capture potentials for the two mills of 95 and 164 kt/a, respectively, and capture and storage costs estimated to 36–60 EUR/tCO2.
Left Atrial Appendage Closure for Primary and Secondary Stroke Prevention in Patients With Hypertrophic Cardiomyopathy and Atrial Fibrillation: A Pilot Study
Authors: Mo Bin-Feng , Zhang Rui , Yuan Jia-Li , Sun Jian , Zhang Peng-Pai , Li Wei , Chen Mu , Cai Xing-Xing , Yu Yi-Chi , Wang Qun-Shan , Li Yi-Gang
Published: October 2021

Background: The aim of this study was to investigate the efficacy of left atrial appendage closure (LAAC) for primary and secondary stroke prevention in patients with hypertrophic cardiomyopathy (HCM) and atrial fibrillation (AF).

Methods: This pilot study enrolled 36 patients with HCM and AF who underwent LAAC between April 2017 and December 2019, of whom 22 were for primary stroke prevention and 14 were for secondary prevention.

Results: The patients enrolled in this study had non-obstructive (86.1%) or mild obstructive (13.9%) HCM. Patients in the Secondary Prevention Group had higher CHA2DS2-VASc scores (5.1 ± 1.4 vs. 2.6 ± 1.6, P < 0.001) and higher HAS-BLED scores (2.8 ± 1.0 vs. 1.5 ± 0.9, P < 0.001) compared with those in the Primary Prevention Group. Successful closure with satisfactory seals (residual leak ≤ 5 mm) was achieved in all patients, with complete occlusion in 86.4% of the Primary Prevention Group and 92.9% of the Secondary Prevention Group. Procedural-related complications included one pericardial effusion and one groin hematoma. One device-related thrombus was identified in the Secondary Prevention Group and resolved after anticoagulation. During a mean follow-up time of 28.4 months, one bleeding event was recorded. There were no thromboembolic events or deaths in either group, with 97.2% of the patients achieving freedom from anticoagulation therapy.

Conclusions: Initial results suggest that LAAC can be a safe and feasible alternative for primary and secondary stroke prevention in selected patients with HCM and AF. Further studies with larger samples are required.
Assessment of Myocardial Function During Blood Pressure Manipulations Using Feature Tracking Cardiovascular Magnetic Resonance
Authors: Fischer Kady , Neuenschwander Mario D. , Jung Christof , Hurni Samuel , Winkler Bernhard M. , Huettenmoser Stefan P. , Jung Bernd , Vogt Andreas P. , Eberle Balthasar , Guensch Dominik P.
Published: October 2021

Background: Coronary autoregulation is a feedback system, which maintains near-constant myocardial blood flow over a range of mean arterial pressure (MAP). Yet in emergency or peri-operative situations, hypotensive or hypertensive episodes may quickly arise. It is not yet established how rapid blood pressure changes outside of the autoregulation zone (ARZ) impact left (LV) and right ventricular (RV) function. Using cardiovascular magnetic resonance (CMR) imaging, measurements of myocardial tissue oxygenation and ventricular systolic and diastolic function can comprehensively assess the heart throughout a range of changing blood pressures.

Design and methods: In 10 anesthetized swine, MAP was varied in steps of 10–15 mmHg from 29 to 196 mmHg using phenylephrine and urapidil inside a 3-Tesla MRI scanner. At each MAP level, oxygenation-sensitive (OS) cine images along with arterial and coronary sinus blood gas samples were obtained and blood flow was measured from a surgically implanted flow probe on the left anterior descending coronary artery. Using CMR feature tracking-software, LV and RV circumferential systolic and diastolic strain parameters were measured from the myocardial oxygenation cines.

Results: LV and RV peak strain are compromised both below the lower limit (LV: Δ1.2 ± 0.4%, RV: Δ4.4 ± 1.2%, p < 0.001) and above the upper limit (LV: Δ2.1 ± 0.4, RV: Δ5.4 ± 1.4, p < 0.001) of the ARZ in comparison to a baseline of 70 mmHg. LV strain demonstrates a non-linear relationship with invasive and non-invasive measures of oxygenation. Specifically for the LV at hypotensive levels below the ARZ, systolic dysfunction is related to myocardial deoxygenation (β = −0.216, p = 0.036) in OS-CMR and both systolic and diastolic dysfunction are linked to reduced coronary blood flow (peak strain: β = −0.028, p = 0.047, early diastolic strain rate: β = 0.026, p = 0.002). These relationships were not observed at hypertensive levels.

Conclusion: In an animal model, biventricular function is compromised outside the coronary autoregulatory zone. Dysfunction at pressures below the lower limit is likely caused by insufficient blood flow and tissue deoxygenation. Conversely, hypertension-induced systolic and diastolic dysfunction points to high afterload as a cause. These findings from an experimental model are translatable to the clinical peri-operative environment in which myocardial deformation may have the potential to guide blood pressure management, in particular at varying individual autoregulation thresholds.
A Pattern of Tolerance Values Transformation by Parents Towards Early Childhood
Authors: Sumadi Tjipto , Abdillah Fauzi , Casmana Asep Rudi
Published: October 2021

This study aims to discover a pattern of the transformation of tolerance values from parents to early childhood. The tolerance value in this day and age experiences some problems in the context of nationality. This can be seen from some parts of the area in Indonesia. For instance, in the traditional village of Cigugur, a life based on tolerance is experienced in the community. This study focused on the Cigugur community’s pattern of applying tolerance values in family and community life. This study uses a qualitative method to undertake the study. Observations and in-depth interviews were used to carry out the data collection, and then it was verified and validated through four stages: member check, audit trial, triangulation, and expert opinion. It can be concluded that the pattern of the transformation of tolerance values from parents to early childhood could be transformed through some stages; 1) making sense of kinship as an adhesive between followers of different religions, 2) conveying religious teachings towards differences, not to be contested but to be respected and admired, 3) avoiding the content of particular political interests, 4) having a sense compassion for each other, and 5) having tepo seliro (respect) for each other.
Influencing Factors of Student Satisfaction With the Teaching Quality of Fundamentals of Entrepreneurship Course Under the Background of Innovation and Entrepreneurship
Authors: Gao Shuai , Zhuang Jiaojiao , Chang Yuteng
Published: October 2021

Innovation and entrepreneurship education is a key way to cultivate applied talents in colleges and universities. This article aimed to optimize the teaching quality of the Fundamentals of Entrepreneurship courses by constructing a conceptual model of “teaching quality–student satisfaction.”Teaching quality is divided into four indicator elements, teaching content, teaching methods, teaching conditions, and teaching management. A student satisfaction questionnaire was designed to measure the teaching quality of a Fundamentals of Entrepreneurship course. The reliability and validity of survey data from Linyi University were analyzed using SPSS.20 software. Through correlation and multiple regression analysis, it can be seen that the teaching content, teaching methods, teaching conditions and teaching management have a significant positive correlation with the teaching quality of the Fundamentals of Entrepreneurship course, which is an important factor affecting student’s satisfaction, and there is a certain gap between the expectation of teaching quality and student’s satisfaction. On this basis, suggestions are put forward to improve student satisfaction with the teaching quality of Fundamentals of Entrepreneurship courses, and to provide empirical evidence and recommendations for continuously improving the teaching quality of Fundamentals of Entrepreneurship courses, thereby improving college students’ employment and entrepreneurship ability.
Changes in Expressions of Spermatogenic Marker Genes and Spermatogenic Cell Population Caused by Stress
Authors: Tian Pengxiang , Zhao Zhiming , Fan Yanli , Cui Na , Shi Baojun , Hao Guimin
Published: October 2021

Many young adults are in a state of stress due to social and psychological pressures, which may result in male reproductive dysfunction. To provide new insight into this phenomenon, we investigated the effect of stress on the regulation of key genes and biological events in specific stages of spermatogenesis. After establishing rat stress models of different time durations, we observed pathological changes in testis through haematoxylin and eosin staining, and analysed gene expression in testis by RNA-seq, bioinformatic analysis, and reverse transcription qPCR (RT-qPCR). Immunohistochemistry (IHC) with the TissueFAXS quantitative imaging system was used to verify changes of different population of spermatogenic cells marked by differentially expressed marker genes. Our results showed that prolonged stress can lead to pathological changes in the testes, such as thinning of the spermatogenic epithelium, a decreased number of spermatogenic epithelial cells, the disordered arrangement of spermatogenic cells, and a decreased number of mature sperms. RNA-seq revealed that key marker spermatogenesis-related genes such as Stra8, Sycp3, Piwil1, and Tnp1 had significantly decreased expression levels in chronic stress groups, and this was confirmed by RT-qPCR and IHC. Collectively, these findings suggest that chronic stress causes damaging pathological changes in testis and dysregulates the marker genes of specific stages of spermatogenesis and change the population of spermatogenic cells, which may be a critical responsible for male reproductive dysfunction.
A Novel Hypothesis: A Role for Follicle Stimulating Hormone in Abdominal Aortic Aneurysm Development in Postmenopausal Women
Authors: Tedjawirja Victoria N. , Nieuwdorp Max , Yeung Kak Khee , Balm Ron , de Waard Vivian
Published: October 2021

An abdominal aortic aneurysm (AAA) is a dilatation of the abdominal aorta, which can potentially be fatal due to exsanguination following rupture. Although AAA is less prevalent in women, women with AAA have a more severe AAA progression compared to men as reflected by enhanced aneurysm growth rates and a higher rupture risk. Women are diagnosed with AAA at an older age than men, and in line with increased osteoporosis and cardiovascular events, the delayed AAA onset has been attributed to the reduction of the protective effect of oestrogens during the menopausal transition. However, new insights have shown that a high follicle stimulating hormone (FSH) level during menopause may also play a key role in those diseases. In this report we hypothesize that FSH may aggravate AAA development and progression in postmenopausal womenviaa direct and/or indirect role, promoting aorta pathology. Since FSH receptors (FSHR) are reported on many other cell types than granulosa cells in the ovaries, it is feasible that FSH stimulation of FSHR-bearing cells such as aortic endothelial cells or inflammatory cells, could promote AAA formation directly. Indirectly, AAA progression may be influenced by an FSH-mediated increase in osteoporosis, which is associated with aortic calcification. Also, an FSH-mediated decrease in cholesterol uptake by the liver and an increase in cholesterol biosynthesis will increase the cholesterol level in the circulation, and subsequently promote aortic atherosclerosis and inflammation. Lastly, FSH-induced adipogenesis may lead to obesity-mediated dysfunction of the microvasculature of the aorta and/or modulation of the periaortic adipose tissue. Thus the long term increased plasma FSH levels during the menopausal transition may contribute to enhanced AAA disease in menopausal women and could be a potential novel target for treatment to lower AAA-related events in women.
Acute Illness and Death in Children With Adrenal Insufficiency
Authors: Worth Chris , Vyas Avni , Banerjee Indraneel , Lin Wei , Jones Julie , Stokes Helen , Komlosy Nicci , Ball Steven , Clayton Peter
Published: October 2021

Background

Adrenal Insufficiency (AI) can lead to life-threatening Adrenal Crisis (AC) and Adrenal Death (AD). Parents are trained to prevent, recognise and react to AC but there is little available information on what parents are actually doing at home to manage symptomatic AI.

Methods

Three approaches were taken: (A) A retrospective analysis of patient characteristics in children and young people with AD over a 13-year period, (B) An interview-aided questionnaire to assess the circumstances around AC in children currently in our adrenal clinic, and (C) a separate study of parent perceptions of the administration of parenteral hydrocortisone.

Results

Thirteen patients died (median age 10 years) over a thirteen-year period resulting in an estimated incidence of one AD per 300 patient years. Those with unspecified adrenal insufficiency were overrepresented (P = 0.004).

Of the 127 patients contacted, thirty-eight (30%) were identified with hospital attendance with AC. Responses from twenty patients (median age 7.5 years) with AC reported nausea/vomiting (75%) and drowsiness (70%) as common symptoms preceding AC. All patients received an increase in oral hydrocortisone prior to admission but only two received intramuscular hydrocortisone.

Questionnaires revealed that 79% of parents reported confidence in the administration of intramuscular hydrocortisone and only 20% identified a missed opportunity for injection.

Conclusions

In children experiencing AC, parents followed ‘sick day’ guidance for oral hydrocortisone, but rarely administered intramuscular hydrocortisone. This finding is discrepant from the 79% of parents who reported confidence in this task. Local training programmes for management of AC are comprehensive, but insufficient to prevent the most serious crises. New strategies to encourage use of parenteral hydrocortisone need to be devised.
Estimating the Societal Benefits of Satellite Instruments: Application to a Break-even Analysis of the GeoXO Hyperspectral IR Sounder
Authors: Lauer Christopher , Conran Joseph , Adkins Jeffery
Published: October 2021

This paper describes the process, and lessons learned in a preliminary benefits study of a proposed infrared hyperspectral sounder (HSS) for NOAA’s next generation geostationary satellite program (GeoXO). The valuation of government-owned satellite systems providing a public good with a complex array of instruments is a nascent field of study. Many different sensor configurations are possible, but there are restrictions due to physical and budget constraints. Accounting for economic considerations during the design and planning phase for satellite constellations helps to ensure that the most cost effective instruments are selected. To assess whether the HSS instrument should be included on GeoXO, we applied a value of information approach and found the benefits associated with this instrument are likely to substantially outweigh the costs. Value of information studies often focus on data and information that has a direct use case. Estimating benefits for the HSS is especially challenging because data are not used directly by decision makers. Instead these data along with information from other Earth observing (EO) satellites play a key role in producing the inputs necessary for modern numerical hydrometeorological modeling. We describe strategies to assess the marginal (i.e., incremental) contribution of an instrument that is part of a complex information production process. We make several recommendations that, if implemented, would improve the quality of future studies of this kind. This includes (1) a systems approach to observing system planning, (2) improving the design of observing system experiments (OSSE and OSE), and (3) better tracking of the decisions and needs of end-users, especially those external to the agency.
Litter Flammability of 50 Southeastern North American Tree Species: Evidence for Mesophication Gradients Across Multiple Ecosystems
Authors: Varner J. Morgan , Kane Jeffrey M. , Kreye Jesse K. , Shearman Timothy M.
Published: October 2021

Widespread fire exclusion and land-use activities across many southeastern United States forested ecosystems have resulted in altered species composition and structure. These changes in composition and structure have been implicated in positive fire-vegetation feedbacks termed “mesophication” where fire spread and intensity are diminished. In forests and woodlands, inherent flammability of different species is the mechanistic driver of mesophication. To date, there has been limited work on documenting the high diversity of flammability among species in the region, limiting the ability to differentiate among species to restore fuels that sustain fire regimes. Here, we coalesce disparate flammability data and add missing species across the spectrum from species that facilitate fire (so called “pyrophytes”) to those that dampen fire (so called “mesophytes”). We present data on 50 important tree species from across the southeast, all burned using identical laboratory methods. We divide our results for four dominant ecosystems: Coastal Plain uplands, oak-hickory woodlands, Appalachian forests, and bottomland forests. Across ecosystems, the most flammable species were American chestnut (Castanea dentata), a suite of pines (Pinus palustris, P. elliottii, P. serotina, and P. rigida), several oaks (Q. laevis, Q. falcata, Q. margaretta, and Q. alba), and sourwood (Oxydendrum arboreum). At the mesophytic end, the least flammable species were Tsuga canadensis, Acer rubrum, and several other hardwoods previously implicated in mesophication. Each of the four ecosystems we studied contained species that spanned the pyrophytic to mesophytic gradient. These data fill in some key holes in our understanding of southeastern fire adaptations, but also provide context for restoration decisions and fire management prioritization efforts to restore and sustain fire-prone ecosystems of the region.
The Application of CRISPR/Cas Systems for Antiviral Therapy
Authors: Baddeley Helen J. E. , Isalan Mark
Published: October 2021

As CRISPR/Cas systems have been refined over time, there has been an effort to apply them to real world problems, such as developing sequence-targeted antiviral therapies. Viruses pose a major threat to humans and new tools are urgently needed to combat these rapidly mutating pathogens. Importantly, a variety of CRISPR systems have the potential to directly cleave DNA and RNA viral genomes, in a targeted and easily-adaptable manner, thus preventing or treating infections. This perspective article highlights recent studies using different Cas effectors against various RNA viruses causing acute infections in humans; a latent virus (HIV-1); a chronic virus (hepatitis B); and viruses infecting livestock and animal species of industrial importance. The outlook and remaining challenges are discussed, particularly in the context of tacking newly emerging viruses, such as SARS-CoV-2.
Menstrual Cycle Phase, Hormonal Contraception, and Alcohol Consumption in Premenopausal Females: A Systematic Review
Authors: Warren Jasmine G. , Fallon Victoria M. , Goodwin Laura , Gage Suzanne H. , Rose Abigail K.
Published: October 2021

Women may be particularly vulnerable to alcohol harm, but many current theories fail to acknowledge the unique factors that influence female alcohol use. The biological mechanisms underlying female alcohol consumption have largely been unexplored, although recently the menstrual cycle has been highlighted as a potentially important factor. This systematic review, using a narrative synthesis, examined the association between the menstrual cycle phases on alcohol consumption and aimed to determine whether hormonal contraception influences this association. The review follows PRISMA and SWiM guidelines, registration number: CRD42018112744. Electronic searches were conducted in the relevant databases with keyword (e.g., “menstrua*”; “alcohol”). Thousand six hundred and sixty-two titles were identified, 16 of which were included in the review. Results were inconsistent regarding whether an association between menstrual cycle phase and alcohol consumption was found. Furthermore, there was inconsistency regarding which phase was associated with higher consumption, and different factors were reported to have moderated the direction, e.g., family history of alcohol use disorder (AUD), premenstrual syndrome (PMS). These conflicting results may be partly explained by variability in both study quality and design, and differences in measurement of cycle phase and alcohol consumption. More robust research is needed before conclusions can be drawn with regard to the role of the menstrual cycle and hormonal contraception on female drinking behavior. This review provides recommendations to strengthen research in this area.
Determinants of Innate Immunity in Visceral Leishmaniasis and Their Implication in Vaccine Development
Authors: Volpedo Greta , Pacheco-Fernandez Thalia , Bhattacharya Parna , Oljuskin Timur , Dey Ranadhir , Gannavaram Sreenivas , Satoskar Abhay R. , Nakhasi Hira L.
Published: October 2021

Leishmaniasis is endemic to the tropical and subtropical regions of the world and is transmitted by the bite of an infected sand fly. The multifaceted interactions betweenLeishmania, the host innate immune cells, and the adaptive immunity determine the severity of pathogenesis and disease development.Leishmaniaparasites establish a chronic infection by subversion and attenuation of the microbicidal functions of phagocytic innate immune cells such as neutrophils, macrophages and dendritic cells (DCs). Other innate cells such as inflammatory monocytes, mast cells and NK cells, also contribute to resistance and/or susceptibility toLeishmaniainfection. In addition to the cytokine/chemokine signals from the innate immune cells, recent studies identified the subtle shifts in the metabolic pathways of the innate cells that activate distinct immune signal cascades. The nexus between metabolic pathways, epigenetic reprogramming and the immune signaling cascades that drive the divergent innate immune responses, remains to be fully understood inLeishmaniapathogenesis. Further, development of safe and efficacious vaccines against Leishmaniasis requires a broader understanding of the early interactions between the parasites and innate immune cells. In this review we focus on the current understanding of the specific role of innate immune cells, the metabolomic and epigenetic reprogramming and immune regulation that occurs during visceral leishmaniasis, and the strategies used by the parasite to evade and modulate host immunity. We highlight how such pathways could be exploited in the development of safe and efficaciousLeishmaniavaccines.