bluuebunny/crossref_metadata_2025 huggingface dataset:
New positions for peptide presentation in Potato virus X capsid protein
Authors: Vaculik Petr , Plchova Helena , Moravec Tomas , Cerovska Noemi
Published: March 2015

Potato virus X (PVX) is widely used as a peptide
presentation system in plant biotechnology, mostly for
transient expression of desired peptides fused to the
N-terminus of its capsid protein (CP). Here we describe
testing/investigation of new positions for peptide
presentation based on seven putative surface loops of
PVX CP. We performed bacterial expression of fourteen
different PVX CPs modified by the insertion of the epitope
derived from the E7 oncoprotein (E7 epitope) of Human
papillomavirus type 16 fused with His tag. The expression
from vector pMPM-A4Ω in Escherichia coli MC1061 was
performed to evaluate the capacity of the PVX CP platform
to tolerate the insertion of E7 epitope fused with His tag
in the seven putative surface loops. The immunological
characteristics of expressed epitopes were assessed
by Western blot using both anti-PVX CP and anti-His
antibodies and by immunoelectron microscopy.
Daily dynamics of photosynthetic parameters
in beech population under periodical drought
conditions
Authors: Borišev Milan , Horak Rita , Pajević Slobodanka , Orlović Saša , Nikolić Nataša , Župunski Milan , Pilipović Andrej
Published: March 2015

The paper presents the impact of periodic
soil drought on physiological parameters important
for bioproductivity of mountain beech populations.
The investigated forest population was located near
Fruška gora mountain peak, where water runs off quickly,
and consequently lack of soil humidity develops very
often. Decreasing trends of photosynthesis, transpiration,
water use efficiency and stomatal conductance (gs) during
the growing season were evident, in correlation with a
shortage of precipitation. Diurnally, photosynthesis of
beech leaves showed rhythmical changes. It was the most
intensive in the morning, then decreased between noon
and 1 pm, and increased again during early afternoon.
High leaf temperature and water deficit in the deeper
soil layers caused a decreasing trend in photosynthesis
and daily rhythmic changes of the transpiration rate and
water use efficiency. Although surface soil water capacity
did not show a significant decreasing trend from July to
September, a lack of precipitation was observed, which
probably caused a lack of moisture in deeper soil layers,
resulting in a decline in photosynthesis and transpiration.
Physiological status, linked to primary photosynthetic
productivity of forests, could be a significant indicator of
environmental conditions and trends in climate changes.
Determination of 21-hydroxylase autoantibodies: inter-laboratory concordance in the Euradrenal International Serum Exchange Program
Authors: Falorni Alberto , Bini Vittorio , Betterle Corrado , Brozzetti Annalisa , Castaño Luis , Fichna Marta , Kämpe Olle , Mellgren Gunnar , Peterson Pärt , Chen Shu , Rönnelid Johan , Seissler Jochen , Tiberti Claudio , Uibo Raivo , Yu Liping , Lernmark Åke , Husebye Eystein
Published: March 2015

21-Hydroxylase autoantibodies (21OHAb) are markers of an adrenal autoimmune process that identifies individuals with autoimmune Addison’s disease (AAD). Quality and inter-laboratory agreement of various 21OHAb tests are incompletely known. The objective of the study was to determine inter-laboratory concordance for 21OHAb determinations.

Sixty-nine sera from 51 patients with AAD and 51 sera from 51 healthy subjects were blindly coded by a randomization center and distributed to 14 laboratories that determined 21OHAb, either by an “in-house” assay (n=9) using in vitro-translated

Intra-assay coefficient of variation ranged from 2.6% to 5.3% for laboratories using the commercial kit and from 5.1% to 23% for laboratories using “in-house” assays. Diagnostic accuracy, expressed as area under ROC curve (AUC), varied from 0.625 to 0.947 with the commercial kit and from 0.562 to 0.978 with “in-house” methods. Cohen’s κ of inter-rater agreement was 0.603 among all 14 laboratories, 0.691 among “in-house” laboratories, and 0.502 among commercial kit users. Optimized cutoff levels, calculated on the basis of AUCs, increased the diagnostic accuracy of every laboratory (AUC >0.9 for 11/14 laboratories) and increased the Cohen’s κ of inter-rater agreement. Discrepancies in quantitation of 21OHAb levels among different laboratories increased with increasing autoantibody levels.

The quality of 21OHAb analytical procedures is mainly influenced by selection of cutoff value and correct handling of assay materials. A standardization program is needed to identify common standard sera and common measuring units.
Combined silencing expression of MGMT with EGFR or galectin-1 enhances the sensitivity of glioblastoma to temozolomide
Authors: Messaoudi Khaled , Clavreul Anne , Danhier Fabienne , Saulnier Patrick , Benoit Jean-Pierre , Lagarce Frederic
Published: March 2015

For several years, the first line of treatment of glioblastoma (GB) patients is based on surgical resection followed by fractioned radiotherapy with concomitant and adjuvant chemotherapy with temozolomide (TMZ). The effectiveness of this treatment is very limited due to the development by tumor cells of mechanisms of resistance to TMZ such as over-expression of O6-methylguanine DNA methyltransferase (MGMT), epidermal growth factor receptor (EGFR) and galectin-1. In this study, we hypothesized that the targeting of MGMT, EGFR and galectin-1 (alone or in combination) by specifics siRNAs carried by chitosan-lipid nanocapsules (chitosan-LNCs) could enhance the sensitivity of U87MG cells to TMZ. We showed in vitro that (i) anti-MGMT and (ii) anti-EGFR or anti-galectin-1 siRNAs decreased significantly the expression of their corresponding proteins and increased the sensitivity of U87MG cells to TMZ. Additionally, the sensitivity of U87MG/MGMT- cells to TMZ was significantly increased when anti-EGFR and anti-galectin-1 siRNAs were combined with a percentage of living cells of 17.8±1.6% at 0.5 mg/mL concentration of TMZ. The combination of anti-MGMT siRNAs with either anti-EGFR or anti-galectin-1 siRNAs enhanced the sensitivity of U87MG/MGMT+ cells to TMZ in comparison to their separately use. No difference was observed between the association of the three siRNAs and other associations. At 0.5 mg/mL concentration of TMZ, the percentage of living cells decreased from 55.1±1.9% to 36.0±4.1% for anti-MGMT alone and the combination of anti-MGMT/anti-galectin-1/anti-EGFR siRNAs, respectively. These siRNA nanovectors represent a good alternative to enhance the effectiveness of the standard treatment of GB. This method could be implemented in future preclinical models for experimental cancer treatment of GB.
Tributylhexadecylphosphonium bromide: an efficient reagent system for the one-pot synthesis of 2,4,5-trisubstituted imidazoles
Authors: Heidari Mohammad , Rezaei Mohsen , Badri Rashid
Published: March 2015

The reaction of benzil, an aromatic aldehyde, and ammonium acetate in ethanol at reflux in the presence of tributylhexadecylphosphonium bromide as catalyst affords a 2,4,5-trisubstituted imidazole. The present methodology offers several advantages over the literature methods, including excellent yields, shorter reaction times, environmentally benign milder reaction conditions, cost-effectiveness of catalyst, easy workup, and purification of products by nonchromatographic methods.
Synthesis and properties of multifunctional nitroxyl radical hindered-amine light stabilizers
Authors: Zhang Wen , Liu Dong , Hua Chengwen , Zhao Junlong , Chen Bang , Gou Xiaofeng
Published: March 2015

A series of novel nitroxyl radical hindered-amine light stabilizer monomers were designed and synthesized from 2,2,6,6-tetramethylpiperidine-N-oxyl-4-ol, cyanuric chloride, allylamine, and 2-hydroxyphenylbenzotriazole derivatives. Structures of the new stabilizers were confirmed by 1H NMR, 13C NMR, FT-IR, ESI-HRMS, and element analysis. The synthesized compounds 5a–f were evaluated for their antioxidant and antiaging properties, and a significant stabilizing effect against photodegradation was found.
Wettability of organically coated tridymite surface – molecular dynamics study
Authors: Šolc Roland , Tunega Daniel , Gerzabek Martin H. , Woche Susanne K. , Bachmann Jörg
Published: March 2015

Classical molecular dynamics (MD) study was performed in order to explain a different wettability of silanized silica-glass surfaces prepared by using two different precursors – dichlorodimethylsilane (DCDMS) and dimethyldiethoxysilane (DMDES), respectively. Whereas the modified surface prepared by DCDMS becomes hydrophobic (contact angle (CA) of water >90°), DMDES-modified surface stays partially hydrophilic (CA ∼39°). In order to explain the observed discrepancy, several models of surfaces of trydimite with different coating by (CH3)2–Si= units were constructed and treated by water nanodroplets in the MD simulations. The models of surfaces differ by a different degree of surface coverage and/or oligomerized (CH3)2–Si= units in a lateral dimension. The simulations showed that incomplete coverage leads to a decrease of the computed CA, whereas upon lateral oligomerization the CA increases. This variation of the CA is directly related to the accessible amount of the hydroxyl groups on the surfaces and can be a possible explanation of the difference in wettability between DCDMS- and DMDES-treated glass surfaces.
Effects of poly(styrene-co-maleic anhydride) on the performance of LGF/TPU/ABS composites
Authors: Zhou Wulei , Xiao Jian , Liu Fang , Qin Shuhao
Published: March 2015

A series of long glass fiber-reinforced thermoplastic polyurethane (TPU) elastomers and acrylonitrile-butadiene-styrene (ABS) copolymer (LGF/TPU/ABS) composites were prepared using self-designed impregnation device. Poly(styrene-co-maleic anhydride) was employed to increase the compatibility between glass fiber and matrix resin and thus obtain the excellent properties of composites. The dynamic mechanical properties of the composites have been investigated using dynamic mechanical thermal analysis. The results indicated that the storage modulus of the composites gradually increases and then decreases with increasing compatibilizer content. In addition, the low temperature peak of the composites shifts to higher temperature with increasing compatibilizer content. The thermal properties of the composites were studied by thermogravimetric analyzer. In addition, the morphology and mechanical properties of the composites are investigated by scanning electron microscopy, a universal testing machine, and a ZBC-4 Impact Pendulum.
Comprehensive establishment and characterization of orthoxenograft mouse models of malignant peripheral nerve sheath tumors for personalized medicine
Authors: Castellsagué Joan , Gel Bernat , Fernández‐Rodríguez Juana , Llatjós Roger , Blanco Ignacio , Benavente Yolanda , Pérez‐Sidelnikova Diana , García‐del Muro Javier , Viñals Joan Maria , Vidal August , Valdés‐Mas Rafael , Terribas Ernest , López‐Doriga Adriana , Pujana Miguel Angel , Capellá Gabriel , Puente Xose S , Serra Eduard , Villanueva Alberto , Lázaro Conxi
Published: March 2015

Malignant peripheral nerve sheath tumors (MPNSTs) are soft‐tissue sarcomas that can arise either sporadically or in association with neurofibromatosis type 1 (NF1). These aggressive malignancies confer poor survival, with no effective therapy available. We present the generation and characterization of five distinct MPNST orthoxenograft models for preclinical testing and personalized medicine. Four of the models are patient‐derived tumor xenografts (PDTX), two independent MPNSTs from the same NF1 patient and two from different sporadic patients. The fifth model is an orthoxenograft derived from an NF1‐related MPNST cell line. All MPNST orthoxenografts were generated by tumor implantation, or cell line injection, next to the sciatic nerve of nude mice, and were perpetuated by 7–10 mouse‐to‐mouse passages. The models reliably recapitulate the histopathological properties of their parental primary tumors. They also mimic distal dissemination properties in mice. Human stroma was rapidly lost after MPNST engraftment and replaced by murine stroma, which facilitated genomic tumor characterization. Compatible with an origin in a catastrophic event and subsequent genome stabilization, MPNST contained highly altered genomes that remained remarkably stable in orthoxenograft establishment and along passages. Mutational frequency and type of somatic point mutations were highly variable among the different MPNSTs modeled, but very consistent when comparing primary tumors with matched orthoxenografts generated. Unsupervised cluster analysis and principal component analysis (PCA) using an MPNST expression signature of ~1,000 genes grouped together all primary tumor–orthoxenograft pairs. Our work points to differences in the engraftment process of primary tumors compared with the engraftment of established cell lines. Following standardization and extensive characterization and validation, the orthoxenograft models were used for initial preclinical drug testing. Sorafenib (a BRAF inhibitor), in combination with doxorubicin or rapamycin, was found to be the most effective treatment for reducing MPNST growth. The development of genomically well‐characterized preclinical models for MPNST allowed the evaluation of novel therapeutic strategies for personalized medicine.
Incidence of primary cancers and intracranial tumour recurrences in GH-treated and untreated adult hypopituitary patients: analyses from the Hypopituitary Control and Complications Study
Authors: Child Christopher J , Conroy Daniel , Zimmermann Alan G , Woodmansee Whitney W , Erfurth Eva Marie , Robison Leslie L
Published: March 2015

Objective

Speculation remains that GH treatment is associated with increased neoplasia risk. Studies in GH-treated childhood cancer survivors suggested higher rates of second neoplasms, while cancer risk data for GH-treated and untreated hypopituitary adults have been variable. We present primary cancer risk data from the Hypopituitary Control and Complications Study (HypoCCS) with a focus on specific cancers, and assessment of recurrence rates for pituitary adenomas (PA) and craniopharyngiomas (CP).

Design

Incident neoplasms during HypoCCS were evaluated in 8418 GH-treated vs 1268 untreated patients for primary malignancies, 3668 GH-treated vs 720 untreated patients with PA history, and 956 GH-treated vs 102 untreated patients with CP history.

Methods

Using population cancer rates, standardised incidence ratios (SIRs) were calculated for all primary cancers, breast, prostate, and colorectal cancers. Neoplasm rates in GH-treated vs untreated patients were analysed after propensity score adjustment of baseline treatment group imbalances.

Results

During mean follow-up of 4.8 years, 225 primary cancers were identified in GH-treated patients, with SIR of 0.82 (95% CI 0.71–0.93). SIRs (95% CI) for GH-treated patients were 0.59 (0.36–0.90) for breast, 0.80 (0.57–1.10) for prostate, and 0.62 (0.38–0.96) for colorectal cancers. Cancer risk was not statistically different between GH-treated and untreated patients (relative risk (RR)=1.00 (95% CI 0.70–1.41), P=0.98). Adjusted RR for recurrence was 0.91 (0.68–1.22), P=0.53 for PA and 1.32 (0.53–3.31), P=0.55 for CP.

Conclusions

There was no increased risk for all-site cancers: breast, prostate or colorectal primary cancers in GH-treated patients during HypoCCS. GH treatment did not increase the risk of PA and CP recurrences.
Role of microenvironment on neuroblastoma SK-N-AS SDHB-silenced cell metabolism and function
Authors: Rapizzi Elena , Fucci Rossella , Giannoni Elisa , Canu Letizia , Richter Susan , Cirri Paolo , Mannelli Massimo
Published: March 2015

In solid tumors, neoplastic cells grow in contact with the so-called tumor microenvironment. The interaction between tumor cells and the microenvironment causes reciprocal metabolic reprogramming and favorable conditions for tumor growth and metastatic spread. To obtain an experimental model resembling the in vivo conditions of the succinate dehydrogenase B subunit (SDHB)-mutated paragangliomas (PGLs), we evaluated the effects of SDHB silencing on metabolism and proliferation in the human neuroblastoma cell line (SK-N-AS), cultured alone or in association with human fibroblasts. Silencing caused a 70% decrease in protein expression, an almost complete loss of the complex specific enzymatic activity, and a significant increase in HIF1α and HIF2α expression; it thus resembled the in vivo tumor cell phenotype. When compared with WT SK-N-AS cells, SDHB-silenced cells showed an altered metabolism characterized by an unexpected significant decrease in glucose uptake and an increase in lactate uptake. Moreover, silenced cells exhibited a significant increase in cell proliferation and metalloproteinase activity. When co-cultured with human fibroblasts, control cells displayed a significant decrease in glucose uptake and a significant increase in cell proliferation as compared with their mono-cultured counterparts. These effects were even more evident in co-cultured silenced cells, with a 70% decrease in glucose uptake and a 92% increase in cell proliferation as compared to their mono-cultured counterparts. The present data indicate for the first time, to our knowledge, that SDHB impairment causes metabolic and functional derangement of neural-crest-derived tumor cells and that the microenvironment, here represented by fibroblasts, strongly affects their tumor metabolism and growth capacity.
Limited Portability of G-Patch Domains in Regulators of the Prp43 RNA Helicase Required for Pre-mRNA Splicing and Ribosomal RNA Maturation in Saccharomyces cerevisiae
Authors: Banerjee Daipayan , McDaniel Peter M , Rymond Brian C
Published: March 2015

The Prp43 DExD/H-box protein is required for progression of the biochemically distinct pre-messenger RNA and ribosomal RNA (rRNA) maturation pathways. In Saccharomyces cerevisiae, the Spp382/Ntr1, Sqs1/Pfa1, and Pxr1/Gno1 proteins are implicated as cofactors necessary for Prp43 helicase activation during spliceosome dissociation (Spp382) and rRNA processing (Sqs1 and Pxr1). While otherwise dissimilar in primary sequence, these Prp43-binding proteins each contain a short glycine-rich G-patch motif required for function and thought to act in protein or nucleic acid recognition. Here yeast two-hybrid, domain-swap, and site-directed mutagenesis approaches are used to investigate G-patch domain activity and portability. Our results reveal that the Spp382, Sqs1, and Pxr1 G-patches differ in Prp43 two-hybrid response and in the ability to reconstitute the Spp382 and Pxr1 RNA processing factors. G-patch protein reconstitution did not correlate with the apparent strength of the Prp43 two-hybrid response, suggesting that this domain has function beyond that of a Prp43 tether. Indeed, while critical for Pxr1 activity, the Pxr1 G-patch appears to contribute little to the yeast two-hybrid interaction. Conversely, deletion of the primary Prp43 binding site within Pxr1 (amino acids 102–149) does not impede rRNA processing but affects small nucleolar RNA (snoRNA) biogenesis, resulting in the accumulation of slightly extended forms of select snoRNAs, a phenotype unexpectedly shared by the prp43 loss-of-function mutant. These and related observations reveal differences in how the Spp382, Sqs1, and Pxr1 proteins interact with Prp43 and provide evidence linking G-patch identity with pathway-specific DExD/H-box helicase activity.
The Ritualization of Consumer Capitalism
Authors: Gonzalez George
Published: March 2015

According to Max Weber, within the differentiated order of modernity, instrumental reason (Zweckrationalität) came to dominate economic life (as well as law, science and architecture). Weber juxtaposed instrumental reason against religious, traditional and affective life. However, the history of consumption, past and present, challenges these distinctions. A major feature of neoliberalism is the increasingly explicit and self reflexive ritualization of consumption, such that paradigms of ritual and consumption appear to cohere. The primary focus of this article does not lie in the mechanics of consumer rituals themselves, as an empirical matter, but, instead, in the conceptual entailments that exist between contemporary discourses of ritual and branding. Taking Starbucks as a case study, the article notes some key similarities between Catherine Bell’s discussion of ritualization and contemporary branding theory and practice, suggesting the possibility that religious studies and marketing share a similar epistemic context which also implies that our own intellectual labors as scholars of religion are enmeshed in the logic and doings of capital. A method for materializing religious studies within contemporary late capitalist conditions, which is deemed urgent, is therefore also proposed.
Systemic Constellation as a Trans-Rational Image of the Unconscious
Authors: Jirasek Ivo , Jiraskova Miroslava
Published: March 2015

“Systemic constellation” is a particular method for achieving a deeper understanding of our life at the level of our unconsciousness. It is based on C.G. Jung’s psychology and we can understand “constellation” in this context as some kind of “role play”. It becomes possible to visualize specific problems or topics, to depict abstract themes or things that oppress us, to identify what is more interesting to us and/or appeals to us more deeply. It is an experiential and existential modus; and it is not easy to put such phenomena into words. However, the substance of the principle of Systemic Constellations is still hidden, some kind of secret. Yet we can meet with something bigger than we are: a kind of wholeness, a system with its bonding and relationships. The article asks whether it is preferable to understand this approach in the framework of “implicit religion,” or of “non-religious spirituality.”
Charakterystyki rynków lokalnych a decyzje banków dotyczące sieci placówek
Authors: Jackowicz Krzysztof , Kowalewski Oskar , Kozłowski Łukasz
Published: March 2015

This paper investigates how local market features influence the decisions taken by Polish banks regarding their branch networks. It was found that during the period 2007–2013 the density of branch networks was highest in relatively rich and urbanized regions. However, the rate of growth in the number of branches was more rapid in regions whose density of branches had hitherto been lower. It was found, moreover, that the presence of an entrepreneurial population had a positive impact on both the density and growth rate of branches.
Wiedza organizacyjna a konkurencyjność banku
Authors: Klimontowicz Monika
Published: March 2015

The turbulent changes in the economic environment and the financial crisis have made conditions for market activity more difficult for banks. Competition on the banking market is turning to hyper-competition – also known as virtual competition. As a result, a bank’s capacity for sustainable development and increased competitiveness is inseparably connected with the ability to respond flexibly to market changes and to make creative use of the resources and experience at its disposal. Organisational knowledge, which is accrued in a process of observation, experience and learning, makes it possible to draw conclusions from the experience gathered, to develop skills, to solve problems and to generate strategic competences. In this way the new challenges presented by the market and customers can be met. The paper presents the role of organizational knowledge in the process of building a bank’s competitiveness. The theoretical approach is enriched by field research, which includes the opinions of bank managers and customers.
Regulacyjne miary płynności w bankach jako dopełnienie miar adekwatności kapitałowej
Authors: Koleśnik Jan
Published: March 2015

The purpose of this paper is to identify liquidity regulation as a complementary element of capital-adequacy measures taken in the European banking system. The paper presents an analysis of the structure and functioning of the standards for liquidity regulation implemented both in the European Union and in Poland. It is the author’s view that the safety of the European banking sector depends on the introduction of both short-term and long-term European Union liquidity regulation measures. Rather than being an alternative to capital-adequacy measures, the liquidity measures will instead complement them. Nevertheless, the method adopted for their implementation will significantly limit the flexibility of banking supervision authorities in the Member States in developing national regulation in this regard.
Konsolidacja sektora bankowego w Polsce – szansa czy zagrożenie?
Authors: Mikołajczyk Marcin
Published: March 2015

The prevailing opinion in the years prior to the recent financial crisis was that extensive consolidation of the banking sector is a good solution in a time of economic slowdown. Creating large banking structures was justified by possible economies of scale connected with mergers and acquisitions. A further argument was that larger banks can diversify their activities and therefore better adapt to changing market conditions – as well as increase their resistance to potential banking crises. As a consequence, banking sector concentration has become an important issue both in discussions and in the literature. Yet no clear answer regarding the optimal structure of the banking sector has been provided. Taking into account changes in the domestic banking market, this article describes the phenomenon of concentration, sets out possible directions for future developments and discusses their potential consequences.
Zastosowanie kowenantów finansowych w bankowości korporacyjnej
Authors: Niedziółka Paweł
Published: March 2015

Financial covenants are tools used to monitor the economic and financial performance of corporate debtors. Their fulfilment is a kind of confirmation of the implementation of financial projections analysed at the processing stage of credit applications. In turn, their violation offers objective proof that an obligor’s financial standing has deteriorated. It is expected that the increasing competition in corporate banking will see extensive implementation of financial covenants, as they improve the quality of monitoring, reduce the operational risk involved in proving that a debtor’s credit rating has deteriorated (thus providing grounds for terminating credit agreements), are a source of additional income for banks and allow flexible adjustment of the price according to the risk level.
Wpływ nowych technologii i kanałów sprzedaży usług bankowych na model biznesowy banku na przykładzie Alior Sync
Authors: Styn Igor
Published: March 2015

With reference to Alior Sync, the first fully virtual bank to operate in Poland, the paper describes the new trends and technologies shaping business models for selling financial products and emphasises the considerable growth potential for Internet banking both in Poland and throughout the world. Multichannel banking is treated as the basic banking business model for the purposes of the paper. Social media banking and banking that is fully virtual are distinguished. The paper regards new technologies as those that generate new, hitherto unavailable functionality in Internet banking, including marketanalysis tools serving to tailor the services offered by banks to shifts in demand.
Clinical Evaluation of Residual Tetrasodium Pyrophosphate Released from Two Different Anticalculus Flosses
Authors: Pedrazzi Vinícius , Corsi Leandro Pereira , Pedrazzi Hamilton , Netto Emilson I. , Nascimento Cássio do , Issa João Paulo Mardegan
Published: March 2015

The aim of this study was to compare the residual content of tetrasodium pyrophosphate released by two different anticalculus dental flosses (Reach PP(r) - entangled polypropylene floss and Reach NT(r) - texturized nylon) in the oral cavity. Ten healthy individuals (aged between 18 and 30 years) were enrolled in this randomized crossover clinical investigation. Participants received instructions on daily dental flossing and the interventions were randomly performed in 2 different groups (NT or PP) of five individuals each according to the dental flosses. Individuals were instructed to use each dental floss with a total of six slides on the two interproximal aspects of target teeth (3 slides on each interproximal aspect). A washout period of one week was used before start flossing interventions and after each type of dental floss to prevent any bias related to the exposure to any product that contained the active ingredient. Samples were collected by #35 sterilized absorbent paper points from interdental fluid after flossing and assessed by ion chromatography. The levels of residual tetrasodium pyrophosphate were evaluated by means of binomial generalized linear model proportions and canonical link function. Both dental flosses were effective in tetrasodium pyrophosphate release at therapeutic levels in the interdental gingival crevicular fluid for a period of up to 2 h after use. No significant differences were found between both groups (p>0.05). It may be concluded that both material composition and physical structure of the new dental floss did not affect the release or the maintenance of anticalculus agent at therapeutic levels for a period of up to 2 h after single use.
Antifungal Susceptibility In Vitro Determined by the Etest(r) for Candida Obtained from the Oral Cavity of Irradiated and Elderly Individuals
Authors: Freitas Edimilson Martins de , Monteiro Larissa Cavalcanti , Fernandes Michelle Bonfim da Silva , Martelli Junior Hercílio , Bonan Paulo Rogério Ferreti , Nobre Sergio Avelino Mota
Published: March 2015

This study aimed to evaluate the in vitro antifungal susceptibility of Candida species of head-and-neck-irradiated patients (Group 1), non-institutionalized (Group 2) and institutionalized elders (Group 3) using Etest(r) methodology. Candida was isolated from saliva and presumptively identified by CHROMagar Candida(r), confirmed by morphological criteria, carbohydrate assimilation (API 20C AUX(r)) and genetic typing (OPE 18). The collection was made from 29, 34 and 29 individuals (Groups 1, 2 and 3, respectively) with 67 isolates. Etest(r) strips (ketoconazole, itraconazole, fluconazole, amphotericin B and flucytosine) on RPMI (Roswell Park Memorial Institute) agar, on duplicate, were used to evaluate susceptibility. ATTC (American Type Culture Collection) 10231 (Candida albicans) was used as quality control. Among the 67 isolates of Candida species, most were susceptible to azoles, flucytosine and amphotericin B. None of the isolates showed resistance and dose-dependent susceptibility to amphotericin B. There were nine strains resistant to itraconazole, six to fluconazole and two to ketoconazole and ten dose-dependent, mainly to flucytocine. The highest MIC (minimum inhibitory concentration) to C. albicans, C. tropicalis, C. parapsilosis was 2.671 μg.mL-1, 8.104 μg.mL-1, 4.429 μg.mL-1, all for flucytosine. C. krusei and C. glabrata were associated with higher MIC for azoles and C. glabrata with higher MIC to flucytosine. In summary, susceptibility to all tested antifungal agents was evident. The isolates were more resistant to itraconazole and dose-dependent to flucytosine. A comparison of C. albicans in the three groups showed no outliers. Higher MIC was associated with C. krusei and C. glabrata.
Is the Masticatory Function Changed in Patients with Temporomandibular Disorder?
Authors: Rodrigues Carolina Almeida , Melchior Melissa de Oliveira , Magri Laís Valencise , Mestriner Jr. Wilson , Mazzetto Marcelo Oliveira
Published: March 2015

Patients with temporomandibular disorders (TMD) often complain and have limitation in masticatory function, which can be affected by a complex interaction of factors. The aim of this study was analyze the masticatory function in patients with TMD using surface electromyography (EMG) and masticatory efficiency (ME). Twenty-seven patients with TMD and 25 considered control (n), aged between 18 and 60 years, paired by age and gender, were evaluated according to RDC/TMD. In both groups were performed: EMG with chewing gum, clinical evaluation of habitual chewing with stuffed cookie (CE) (number of chewing strokes and time) and analysis of ME with fuchsin beads. Nonparametric statistical analyses were used (Mann-Whitney) for comparisons between groups, with 5% significance level. For all variables, the TMD group showed higher values than the control, with statistical significance for ME (p<0.0001), number of chewing strokes (p=0.04), chewing time (p=0.009), right masseter EMG activity (p=0.05), left masseter (p=0.005), right anterior temporal (p=0.05) and left anterior temporal (p=0.001). The conclusion is that patients with TMD showed changed chewing pattern, but without impairment of masticatory function.
Surface Characteristics of Reciprocating Instruments Before and After Use - A SEM Analysis
Authors: Hanan Aida Rene Assayag , Meireles Daniely Amorin de , Sponchiado Júnior Emílio Carlos , Hanan Simone , Kuga Milton Carlos , Bonetti Filho Idomeo
Published: March 2015

The presence of debris, defects and deformations of endodontic reciprocating instruments before and after chemical-mechanical preparation (MCP) was analyzed using scanning electron microscopy (SEM). The following 26 instruments were divided into 2 groups: Waveone (n=13) and Reciproc (n=13) and examined by SEM (150 x magnification) prior to canal preparation at 2 and 4 mm from the tip. The instruments were used in the preparation of mesial root canals of 26 extracted human permanent mandibular molars. The instruments were then washed in ultrasonic bath and subjected to new microscopic analysis of debris and deformation by a score that used the presence or absence of irregular edges, grooves, microcavities and burrs as criteria. After the SEM analysis and with the scores of the examiners, the collected data were subjected to descriptive statistical analysis using the Kruskall-Walis and Mann Whitney test at a 5% significance level. All instruments examined presented debris before and after use. A statistically significant difference was found for defects and deformation between the groups (p<0.05). The presence of defects and deformities was higher in the WaveOne instruments, and Reciproc instruments presented a lower rate.
Demineralization and Hydrogen Peroxide Penetration in Teeth with Incipient Lesions
Authors: Briso André Luiz Fraga , Gonçalves Rafael Simões , Costa Fernanda Bernardi da , Gallinari Marjorie de Oliveira , Cintra Luciano Tavares Angelo , Santos Paulo Henrique dos
Published: March 2015

The aim of this study was to evaluate the demineralization and hydrogen peroxide (HP) penetration in teeth with incipient lesions submitted to bleaching treatment. For analysis of HP penetration, sound and demineralized enamel/dentin discs were placed in artificial pulp chambers containing acetate buffer solution. After bleaching treatment, this solution was subjected for analysis of optical density by spectrophotometry and the disc surfaces were analyzed with scanning electron microscopy (SEM) and polarized light microscopy (PLM). The remaining discs were subjected for cross-sectional hardness analysis at different depths. Data were analyzed by repeated measures ANOVA and PLSD Fisher test (a=0.05). It was observed that previously demineralized teeth showed greater HP penetration (p<0.05). The bleaching treatment caused changes to a depth of 20 µm in sound enamel and up to 90 µm in demineralized enamel. SEM and PLM images revealed that the bleaching treatment caused superficial changes that were considerably more accentuated in previously demineralized teeth. It may be concluded that the enamel mineralization level influences HP penetration and the bleaching agent contributed to increase the demineralization depth.
Serum Adipokine Levels and their Relationship with Fatty Acids in Patients with Chronic Periodontitis
Authors: Sete Manuela Rubim Camara , Lira Júnior Ronaldo , Fischer Ricardo Guimarães , Figueredo Carlos Marcelo da Silva
Published: March 2015

Adipokines are present in inflammatory processes and may be directly related to periodontal disease. Moreover, their activities may be regulated by fatty acids. The goal of this study was to quantify the concentrations of the main adipokines, leptin, adiponectin and resistin, and the docosahexaenoic (DHA), docosapentaenoic (DPA), eicosapentaenoic (EPA) and arachidonic (AA) fatty acids, in patients with generalized chronic periodontitis. As a secondary objective, the ratios of these substances in the blood of these patients were evaluated. The study included 15 systemically healthy patients with generalized chronic periodontitis (test group) and 15 patients with gingivitis (control group). Medical and periodontal parameters and blood samples were collected. Serum concentrations of fatty acids were analyzed by gas chromatography and adipokines by multiplex bead immunoassay. There was no significant difference in adipokines between groups. However, there was a tendency for lower values of adiponectin in periodontitis patients. Regarding the fatty acids, they were significantly higher in the test group compared with controls. The res/DHA, res/AA, adipon/DHA, adipon/AA and adipon/DPA ratios were significantly lower in the test group. There was no significant correlation between adipokines and clinical parameters and between adipokines and fatty acids levels. It was concluded that generalized chronic periodontitis patients showed significantly higher levels of fatty acids in comparison to gingivitis; adiponectin revealed a trend to lower values in the periodontitis group, even after Ancova correction. The ratios suggest a minor proportion of adiponectin and resistin in relation to the fatty acids in patients with generalized chronic periodontitis.
Accuracy of Two Cephalometric Analyses in the Treatment of Patients with Skeletal Class III Malocclusion
Authors: Dantas João Frank Carvalho , Carvalho Sergio Henrique Gonçalves de , Oliveira Luciana Soares de Andrade Freitas , Barbosa Danilo Batista Martins , Souza Raphael Freitas de , Sarmento Viviane Almeida
Published: March 2015

The aim of the present study was to compare the treatment plan outlined from the diagnosis obtained by two different cephalometric analyses with the clinical decision made for surgical treatment of patients with skeletal Class III malocclusion. For this purpose, 82 skeletal Class III patients were evaluated, divided into three groups according to the surgical procedure performed for dentofacial deformity correction. Their preoperative cephalometric radiographs were submitted to McNamara and Steiner cephalometric analyses. The association of variables was evaluated by Kappa correlation (α=5%). The agreement between indication of surgical procedures performed based on clinical decision and surgical treatment indicated based on the evaluation of McNamara and Steiner cephalometric analyses were not significant (p=0.609 and p=0.544, respectively). McNamara and Steiner analyses showed reasonable agreement with each other as to the diagnosis, but did not agree with the clinical decision to treat skeletal CIII patients. Both tests were equally inaccurate in the indication of the surgical treatment.
Influence of Inner Circular Sealing Area Impression Method on the Retention of Complete Dentures
Authors: Wang Cun-Wei , Shao Qi , Sun Hui-Qiang , Mao Meng-Yun , Zhang Xin-Wei , Gong Qi , Xiao Guo-Ning
Published: March 2015

The aims of the present study were to describe an impression method of "inner circular sealing area" and to evaluate the effect of the method on retention, aesthetics and comfort of complete dentures, which lack labial base for patients with maxillary protrusions. Three patients were subjected to the experiment, and two sets of complete maxillary dentures were made for each patient; the first set was made without labial base via an inner circular sealing area method (experimental group) and the second had an intact base that was made with conventional methods (control group). Retention force tests were implemented with a tensile strength assessment device to assess the retention and a visual analogue scale (VAS) was used to evaluate the comfort between the two groups. Results showed larger retention force, better aesthetics and more comfort in the experimental group. The improved two-step impression method formed an inner circular sealing area that prevented damage to the peripheral border seal effect of the denture caused by incomplete bases and obtained better denture retention.
Use of a Titanium Mesh "Shelter" Combined with the Soft Tissue Matrix Expansion (Tent Pole) Grafting in the Reconstruction of a Severely Resorbed Edentulous Mandible. Technical Note.
Authors: Sverzut Cássio Edvard , Trivellato Alexandre Elias , Sverzut Alexander Tadeu
Published: March 2015

Patients wearing complete dentures for a long time usually exhibit lack of bone and keratinized mucosa in the bearing area due to bone resorption. The patients suffering from this phenomenon usually have unstable and non-retentive complete denture, which result in constant trauma to the mucosa, pain, functional limitations and worsening of facial esthetics. An innovative technique has been described in which a novel surgical approach using osseointegrated dental implants as "tent poles" was applied concomitant with particulate autogenous bone graft. The authors claim that the control and maintenance of the surgically expanded soft tissue volume should prevent graft resorption in the long term. Nevertheless, resorption of the bone graft is usually more significant where the bone mass is poorer, in the mandibular body. This paper describes a case of severely resorbed edentulous mandible in which the "tent pole" technique was applied with some modifications. Use of the titanium mesh "shelters" and two additional implants was effective in "protecting" the bone graft in the posterior portion of the mandibular body increasing bone mass volume in this area. Furthermore, we believe that this kind of graft "protection" on the whole residual alveolar ridge can increase the width of bone mass gain optimizing the "tent pole" technique.
Surface Treatments for Repair of Feldspathic, Leucite - and Lithium Disilicate-Reinforced Glass Ceramics Using Composite Resin
Authors: Neis Christian Alencar , Albuquerque Nadine Luísa Guimarães , Albuquerque Ivo de Souza , Gomes Erica Alves , Souza-Filho Celso Bernardo de , Feitosa Victor Pinheiro , Spazzin Aloisio Oro , Bacchi Atais
Published: March 2015

The aim of this study was to evaluate the efficacy of different surface conditioning methods on the microtensile bond strength of a restorative composite repair in three types of dental ceramics: lithium disilicate-reinforced, leucite-reinforced and feldspathic. Twelve blocks were sintered for each type of ceramic (n=3) and stored for 3 months in distilled water at 37 °C. The bonding surface of ceramics was abraded with 600-grit SiC paper. Surface treatments for each ceramic were: GC (control) - none; GDB - diamond bur #30 µm; GHF - hydrofluoric acid (10%); GT- tribochemical silica coating (45-μm size particles). Treatments were followed by cleaning with phosphoric acid 37% for 20 s + silane + adhesive. The composite resin was used as restorative material. After repair, samples were subjected to thermocycled ageing (10,000 cycles between 5 °C and 55 °C for 30 s). Thereafter, the samples were sectioned into 1.0 mm2 sticks and tested for microtensile bond strength with 0.5 mm/min crosshead speed. Data were compared by two-way ANOVA and Tukey's test (α=0.05). The superficial wear with diamond bur proved to be suitable for feldspathic porcelain and for leucite-reinforced glass ceramic while hydrofluoric acid-etching is indicated for repairs in lithium disilicate-reinforced ceramic; tribochemical silica coating is applicable to leucite-reinforced ceramic. Predominance of adhesive failures was observed (>85% in all groups). In conclusion, the success of surface treatments depends on the type of ceramic to be repaired.
Saúde e Prevenção nas Escolas (SPE): elementos para avaliação de projetos sociais em Juazeiro, Bahia, Brasil
Authors: Ribeiro Marcelo Silva de Souza , Ribeiro Carla Valois
Published: March 2015

O SPE, à época, apresentado como trabalho de conclusão de curso (TCC) de Residência Multiprofissional em Saúde da Família, pela autora, então discente do programa, representou uma iniciativa de saúde e educação, promovendo uma atividade de saúde sexual e reprodutiva para adolescentes no Município de Juazeiro/BA, Brasil. O objetivo deste trabalho foi analisar o SPE em sua concepção, metodologia e resultados, considerando a intersetorialidade, a interface saúde e educação e a efetividade social. Concluiu-se que foi oportuna a garantia da sustentabilidade do projeto e o fomento à participação da comunidade escolar. Os desdobramentos do SPE são visualizados nas macropolíticas de atenção à saúde e educação que hoje direcionam as principais pactuações intersetoriais deste cenário, demandando a compreensão de sua edificação para seu fortalecimento.
Docentes nóveles y los procesos de recontextualización en contextos de vulnerabilidad
Authors: Lester Luis Ajagan , Sáez Gonzalo , Muñoz Carlos , Rodríguez Guillermo , Córdova Rodrigo Cea
Published: March 2015

En este artículo se sintetizan los resultados de una investigación cualitativa entre profesores nóveles que trabajan en zonas de alta vulnerabilidad social, económica y cultural en la Región del Biobío, Chile. El objetivo central de la investigación fue conocer las interpretaciones y percepciones de los docentes ante los procesos de recontextualización en el aula de planes y programas oficiales. El método consistió en entrevistas cualitativas profundas y el análisis se basó en la técnica ad-hoc. Los resultados demuestran un alto grado de compromiso social por parte de los docentes nóveles con sus estudiantes y una actitud autónoma ante la recontextualización de planes y programas.
El discurso pedagógico oficial en Argentina (2003-2013): trabajo docente e igualdad
Authors: Vassiliades Alejandro
Published: March 2015

Este artículo aborda los resultados de una investigación sobre las regulaciones del trabajo de enseñar en Argentina. Utilizando herramientas conceptuales provenientes del análisis político del discurso, analizaré algunos de los principales elementos de la discursividad oficial en el período 2003-2013. Plantearé que ellos se articularon en torno a la equivalencia discursiva igualdad-inclusión educativa, configurando dos cadenas de significantes emparentadas. Una de ellas asoció la igualdad y la inclusión educativas a la afirmación de la educación como derecho social, la principalidad estatal, la restitución de lo común y la consideración de la diversidad; la otra enlazó la igualdad y la inclusión educativas a la idea de centralidad de la enseñanza, el trabajo con situaciones de "desigualdad educativa" -tales como la sobreedad y la repitencia- y de "vulnerabilidad social", y la promoción de modos organizacionales alternativos.
Pesquisa educacional e experiência humana na perspectiva hermenêutica
Authors: Dalbosco Cláudio A.
Published: March 2015

O ensaio aborda a fragilidade teórica da pesquisa educacional brasileira, desdobrando a argumentação em três seções. Na primeira, baseando-se em Bernardete Gatti, o texto alinhava alguns traços do diagnóstico sobre as dificuldades e os limites do atual estágio da pesquisa educacional no país. Na segunda, concentra-se em resumir o aspecto nuclear da ambiguidade da experiência ordinária, científica e filosófica. Pretende mostrar que, se tais formas foram acompanhadas por tendências dogmáticas, também apresentam concepções abertas e plurais. Na última parte, procura justificar a experiência hermenêutica gadameriana como perspectiva promissora para ampliar a noção de experiência humana, visando a descortinar um novo olhar sobre a natureza da pesquisa educacional.