bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative metabolomic study of fungal foliar endophytes and their long-lived host Astrocaryum sciophilum: a model for exploring the chemodiversity of host-microbe interactions
Authors: Pellissier Leonie , Gaudry Arnaud , Vilette Salomé , Lecoultre Nicole , Rutz Adriano , Allard Pierre-Marie , Marcourt Laurence , Ferreira Queiroz Emerson , Chave Jérôme , Eparvier Véronique , Stien Didier , Gindro Katia , Wolfender Jean-Luc
Published: December 2023

Introduction

In contrast to the dynamics observed in plant/pathogen interactions, endophytic fungi have the capacity to establish enduring associations within their hosts, leading to the development of a mutually beneficial relationship that relies on specialized chemical interactions. Research indicates that the presence of endophytic fungi has the ability to significantly modify the chemical makeup of the host organism. Our hypothesis proposes the existence of a reciprocal exchange of chemical signals between plants and fungi, facilitated by specialized chemical processes that could potentially manifest within the tissues of the host. This research aimed to precisely quantify the portion of the cumulative fungal endophytic community's metabolome detectable within host leaves, and tentatively evaluate its relevance to the host-endophyte interplay. The understory palm Astrocaryum sciophilum (Miq.) Pulle was used as a interesting host plant because of its notable resilience and prolonged life cycle, in a tropical ecosystem.

Method

Using advanced metabolome characterization, including UHPLC-HRMS/MS and molecular networking, the study explored enriched metabolomes of both host leaves and 15 endophytic fungi. The intention was to capture a metabolomic "snapshot" of both host and endophytic community, to achieve a thorough and detailed analysis.

Results and discussion

This approach yielded an extended MS-based molecular network, integrating diverse metadata for identifying host- and endophyte-derived metabolites. The exploration of such data (>24000 features in positive ionization mode) enabled effective metabolome comparison, yielding insights into cultivable endophyte chemodiversity and occurrence of common metabolites between the holobiont and its fungal communities. Surprisingly, a minor subset of features overlapped between host leaf and fungal samples despite significant plant metabolome enrichment. This indicated that fungal metabolic signatures produced in vitro remain sparingly detectable in the leaf. Several classes of primary metabolites were possibly shared. Specific fungal metabolites and/or compounds of their chemical classes were only occasionally discernible in the leaf, highlighting endophytes partial contribution to the overall holobiont metabolome. To our knowledge, the metabolomic study of a plant host and its microbiome has rarely been performed in such a comprehensive manner. The general analytical strategy proposed in this paper seems well-adapted for any study in the field of microbial- or microbiome-related MS and can be applied to most host-microbe interactions.
Effectiveness of electroacupuncture on anxiety: a systematic review and meta-analysis of randomized controlled trials
Authors: Hong Wan ki , Kim Yeon Ji , Lee Ye rim , Jeong Hye In , Kim Kyeong Han , Ko Seong-Gyu
Published: December 2023

This systematic review and meta-analysis aimed to comprehensively evaluate the effectiveness of electroacupuncture (EA) for patients with anxiety. Randomized controlled trials (RCTs) on the treatment of anxiety by EA up to November 2022 were searched and collected from nine databases. Hamilton Anxiety Rating Scale (HAMA), self-rating anxiety scale (SAS), and adverse reactions were used as outcome indicators. The quality of relevant articles was evaluated using the Cochrane Collaboration’s risk of bias tool. The quality of evidence for each outcome was classified as “low risk,” “unclear risk,” or “high risk.” RevMan 5.0 was used for data analysis. A total of 633 articles were identified from nine electronic databases; 37 RCTs were included, which measured anxiety changes by using EA alone compared to the control group. For the main outcome, EA significantly reduced the HAMA score [Mean difference (MD):−1.13 (95% CI:−2.55–0.29), I2:80%], and the quality of evidence was moderate. EA significantly reduced the SAS score (MD:−3.47 (95% CI,−6.57−−0.36), I2:88%), and the quality of evidence was moderate. Our meta-analysis shows that EA reduces HAMA and SAS. This study suggests that EA can relieve anxiety. For various uses, additional research is needed on its effect when combined with other treatments.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=345658, identifier (CRD42022345658).
The impact of organizational politics on work engagement—the mediating role of the doctrine of the mean
Authors: Su Weijian , Xie Chengxuan
Published: December 2023

Introduction

Events Theory posits that the perception of organizational politics affects job attitudes. The purpose of this study was to answer the question: does organizational politics have a similar impact on Chinese individuals who adhere to the doctrine of the mean?

Methods

We collected survey data from 3,059 library personnel in 36 Chinese university libraries and conducted analysis using the Analytic Hierarchy Process.

Results

The results showed that the perception of organizational politics reduces employee work engagement. However, this impact exhibits heterogeneity. For newly recruited employees and employees aged 50 and above who are nearing retirement, the impact of organizational politics on work engagement is relatively small. Conversely, for employees aged 30–50, organizational politics has a significant negative impact. Furthermore, the doctrine of the mean moderates the impact of organizational politics on work engagement, mitigating its negative effects.

Discussion

The conclusions offer new insights into enhancing employee work motivation. The library should take more measures to safeguard employee rights to enhance work motivation.
Investigating the borders of autism spectrum disorder: lessons from the former diagnosis of pervasive developmental disorder not otherwise specified
Authors: Coutelle Romain , Coulon Nathalie , Schröder Carmen M. , Putois Olivier
Published: December 2023

Introduction

Autism Spectrum Disorder (ASD) diagnosis is relatively consensual in typical forms. The margins of the spectrum and their degree of extension, however, are controversial. This has far-reaching implications, which extend beyond theoretical considerations: first, peripheral forms of autism are more prevalent than central forms; second, we do not know how relevant typical-targeted recommendations are for atypical forms. In DSM-IV-TR, these margins of autism were studied within the category of Pervasive Developmental Disorder – Not Otherwise Specified (PDD-NOS). In spite of its low reliability, this former diagnosis was of particular interest to shed light on the gray area of margins. The aim of this systematic is therefore to investigate the clinical characteristics of PDD-NOS in comparison with Autistic Disorder.

Method

A stepwise systematic PRISMA literature review was conducted by searching PubMed and Web Of Science databases to select corresponding studies.

Results

The systematic review included 81 studies comprising 6,644 children with PDD-NOS. Cross-sectional and longitudinal studies comparing PDD-NOS and AD showed that PDD-NOS corresponds to milder form of autism with less impact and less associated disorder, with the exception of schizophrenia and mood disorder.

Discussion

Our review challenges initial views of PDD-NOS, and shows the clinical relevance of this diagnosis when dealing with the margins of autism, and the de facto diversity included in the spectrum. However, in view of the many limitations of PDD-NOS (low reliability, instability through time, low acceptability), we suggest taxonomic changes in DSM-5: we introduce a new category based on three main dimensions related to socialization impairment, emotional lability and psychotic symptoms.

Conclusion

Our review argues for a distinction between AD and PDD-NOS on clinical characteristics and thus highlights the need to study the margins of autism. While the limitations of the PDD-NOS category made it irrelevant to investigate these margins from a research perspective, we believe that a multidimensional approach for mental health professionals taping socialization, emotion lability and psychotic symptoms would be interesting. Our review therefore encourage future studies to test relevant criteria for a new category and possibly identify developmental trajectories, specific interventions and treatments.
Configurations of actual and perceived motor competence among elementary school children in China: differences in physical activity
Authors: Chai Hongqin , Xue Rui , Yao Lei , Miao Miao , Han Bochen
Published: December 2023

Background

Actual motor competence (AMC) and perceived motor competence (PMC), as determinants of children’s active participation in physical activity (PA), were important for children’s healthy development. The correlation between the two had been confirmed. To further understand this relationship, this study investigated the current status of AMC, PMC, and PA in Chinese school-age children, used a person-centered approach to reveal the characteristics of the development of motor competence (MC) in Chinese school-age children and the differences in the level of PA among different MC profiles of children.

Materials and methods

A total of 532 children (age: M = 9.37, SD = 1.80 years-old) from grades 1 to 6 participated in this cross-sectional study (male, n = 284, 53.4%; female, n = 248, 46.6%). The Test of Gross Motor Development-3 (TGMD-3) was used to measure children’s AMC, the Pictorial Scale of Perceived Movement Skill Competence (PMSC) to measure children’s PMC, and the revised Chinese version of Physical Activity Questionnaire for Older Children (PAQ-C) to assess children’s PA levels.

Results

There were some gender differences in AMC but no significant gender differences in PMC and PA in children. AMC and PA levels increased as the children aged, while PMC showed some decline. Cluster analysis identified four groups of children with different MC profiles. Two groups of children had corresponding AMC and PMC levels (the “high-high” cluster, N = 200, 38.91%; the “low-low” cluster, N = 63, 12.26%), and the other two groups were inconsistent in AMC and PMC (the “high-low” cluster, N = 100, 19.46%; the “low-high” cluster, N = 151, 29.38%). Significant differences in PA levels were found between children with different MC profiles. The “high-high” cluster children had the highest PA levels, whereas the “low-low” cluster children demonstrated the lowest PA levels.

Conclusion

AMC, PMC, and PA in Chinese school-age children were consistent with the pattern of child growth and development. Children with high AMC and high PMC usually had high levels of PA. Therefore, it was recommended to seize the best opportunity to intervene with children, and family, school, and community should synergize to help children improve AMC and PMC, and then actively participate in PA.
Pharmacoeconomic evaluation of isavuconazole, posaconazole, and voriconazole for the treatment of invasive mold diseases in hematological patients: initial therapy prior to pathogen differential diagnosis in China
Authors: Han Guangxin , Xu Qing , Lv Qianzhou , Li Xiaoyu , Shi Xiaoping
Published: December 2023

Background

Invasive mold diseases (IMD) is associated with high mortality and a substantial economic burden. For high-risk patients, fever drive or diagnostic drive therapy is usually initiated prior to the differential diagnosis of the pathogen. This study evaluated the cost-effectiveness of isavuconazole, posaconazole, vs. voriconazole in the treatment of IMD from the perspective of the Chinese healthcare system, informing healthcare decision-making and resource allocation.

Methods

A decision analytic model was constructed using TreeAge Pro 2011 software to evaluate the cost-effectiveness of the entire disease course. We assumed that the prevalence of mucormycosis in the patients entering the model was 7.8%. Efficacy, cost, adverse events, and other data included in the model were mainly derived from clinical studies, published literature, and publicly available databases. The primary outcomes of the model output were total cost, quality-adjusted life years (QALYs), life years (Lys), and incremental cost-effectiveness ratio (ICER). The willing-to-pay (WTP) threshold was defined as one to three times China's GDP per capita in 2022. One-way sensitivity analysis and probability sensitivity analysis were used to determine the robustness of the model. At the same time, the cost-effectiveness of three triazole antifungal agents under a broader range of mucormycosis prevalence, when voriconazole was covered by medical insurance reimbursement, and after the price reduction of posaconazole was discussed.

Results

Compared with voriconazole, isavuconazole provided an additional 0.38 Lys (9.29 vs. 8.91 LYs) and 0.31 QALYs (7.62 vs. 7.31 QALYs); ICER was $15,702.46/QALY, well-below the WTP threshold ($38,223/QALY). However, posaconazole did not provide a significant economic advantage over voriconazole (9.40 vs. 9.36 Lys; 7.71 vs. 7.68 QALYs; ICER $64,466.57/QALY). One-way sensitivity analysis found that ICER was highly sensitive to the mortality of patients with invasive aspergillus infection. In the probabilistic sensitivity analysis, when the WTP threshold was $38,223/QALY, the probability of isavuconazole being cost-effective was 72.9%. The scenario analysis results indicated that posaconazole would become cost-effective when the price was reduced by 15% or the prevalence of mucormycosis was 14%.

Conclusions

Isavuconazole represents a cost-effective initial option for treating IMD in high-risk hematological patients prior to the differential diagnosis of pathogens. It will also be economical when a 15% reduction in posaconazole cost is achieved.
Molecular epidemiology of SARS-CoV-2 in Northern South Africa: wastewater surveillance from January 2021 to May 2022
Authors: Tambe Lisa Arrah Mbang , Mathobo Phindulo , Matume Nontokozo D. , Munzhedzi Mukhethwa , Edokpayi Joshua Nosa , Viraragavan Amsha , Glanzmann Brigitte , Tebit Denis M. , Mavhandu-Ramarumo Lufuno Grace , Street Renee , Johnson Rabia , Kinnear Craig , Bessong Pascal Obong
Published: December 2023

Introduction

Wastewater-based genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) provides a comprehensive approach to characterize evolutionary patterns and distribution of viral types in a population. This study documents the molecular epidemiology of SARS-CoV-2, in Northern South Africa, from January 2021 to May 2022.

Methodology

A total of 487 wastewater samples were collected from the influent of eight wastewater treatment facilities and tested for SARS-CoV-2 RNA using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). SARS-CoV-2 positive samples with genome copies/mL ≥1,500 were subjected to allele-specific genotyping (ASG) targeting the Spike protein; 75 SARS-CoV-2 positive samples were subjected to whole genome sequencing (WGS) on the ATOPlex platform. Variants of concern (VoC) and lineages were assigned using the Nextclade and PangoLIN Software. Concordance for VoC between ASG and WGS analyses was determined. Sequence relationship was determined by phylogenetic analysis.

Results

Seventy-five percent (365/487) of the influent samples were positive for SARS-CoV-2 RNA. Delta and Omicron VoC were more predominant at a prevalence of 45 and 32%, respectively, and they were detected as early as January and February 2021, while Beta VoC was least detected at a prevalence of 5%. A total of 11/60 (18%) sequences were assigned lineages and clades only, but not a specific VoC name. Phylogenetic analysis was used to investigate the relationship of these sequences to other study sequences, and further characterize them. Concordance in variant assignment between ASG and WGS was seen in 51.2% of the study sequences. There was more intra-variant diversity among Beta VoC sequences; mutation E484K was absent. Three previously undescribed mutations (A361S, V327I, D427Y) were seen in Delta VoC.

Discussion and Conclusion

The detection of Delta and Omicron VoCs in study sites earlier in the outbreak than has been reported in other regions of South Africa highlights the importance of population-based approaches over individual sample-based approaches in genomic surveillance. Inclusion of non-Spike protein targets could improve the specificity of ASG, since all VoCs share similar Spike protein mutations. Finally, continuous molecular epidemiology with the application of sensitive technologies such as next generation sequencing (NGS) is necessary for the documentation of mutations whose implications when further investigated could enhance diagnostics, and vaccine development efforts.
The factors that influence the growth and performance of micro and small-scale enterprises in Dessie Town administration
Authors: Ebrahim Endris Assen , Toy Ahmet , Kassim Yissa Hassen
Published: December 2023

Introduction

The growth or performance of Micro and Small-Scale Enterprises (MSSEs) is widely recognized for their important contributions to economic developments in developing countries. Small and Medium-sized businesses (MMEs) in most developing nations encounter obstacles throughout and after the start-up process. This research aims to explore the determinants of the growth of MSSEs with a special emphasis on five work sectors: manufacturing, trade, construction, service, and urban agriculture in Dessie Town, Ethiopia.

Methods

The primary data was collected using a self-questionnaire from a sample of 218 managers/owners of MSE operators. Both descriptive and inferential analysis were used to analyze the collected data. Descriptive narrations were used to analyze qualitative data as a concurrent triangulation strategy. The sample respondents were selected using a stratified random sampling method based on the type of business sector. This practical study provoked nine major issues affecting the growth of MSSEs in town: political & legal factors, including bureaucratic bottlenecks system, the COVID-19 pandemic, working premises, market-related issues, road infrastructures, management system, technology-related factors, and entrepreneurial-related factors. The results show that linear and positive substantial to strong significant relationships or associations exist between some independent variables and the growth of MSSEs. Among the expected nine determinants of MSSEs' growth and performance, only political & legal-related, management-related, market-related, technology-related, infrastructure-related, entrepreneur-related factors, and COVID-19 pandemic were statistically significant. Besides, the nominated factors explained the total variations in the MSSEs' growth and performance at a 5% significance level. Realizing this conclusion, the government and non-government bodies and operators of MSSEs should give attention to management, marketing, technology infrastructure, and entrepreneur-related factors.
Cognitive behavioral therapy for insomnia in a military traumatic brain injury clinic: a quality improvement project assessing the integration of a smartphone application with behavioral treatment
Authors: Matsuura Justin T. , Keller Nicole S. , Lustik Michael B. , Campbell Carmen E. , Grills Chad E.
Published: December 2023

Objectives

While the association between insomnia and traumatic brain injury (TBI) is well established, TBI rehabilitation programs that focus on sleep as a primary target are limited. Cognitive behavioral therapy for insomnia (CBTi) is an effective treatment for insomnia, however; its use within TBI clinics is relatively unknown. Therefore, our aim was to evaluate the implementation of CBTi, used in conjunction with a smartphone app for insomnia, within a US military TBI program to improve care within this setting.

Setting

A TBI clinic at a US military installation.

Methods

MHS beneficiaries underwent 6 sessions of CBTi and a 1-month post-treatment follow up session. Data was collected at each treatment session as part of routine clinical care.

Results

A total of 69 US MHS beneficiaries seen at a TBI clinic with a diagnosis of insomnia began CBTi. Attrition rate at the end of the CBTi program and 1-month posttreatment session was 35% and 48%, respectively. Results demonstrated that sleep onset latency (SOL) and wake after sleep onset (WASO) decreased during treatment (p's < 0.001). Further, symptoms reported on the Insomnia Severity Index (ISI) improved during CBTi (p < 0.001).

Conclusion

Findings demonstrate how CBTi used in conjunction with a CBTi smartphone application can be used to effectively treat insomnia for MHS beneficiaries seeking care for TBIs. This evaluation provides the basis for further research on how CBTi may improve care within TBI programs.
Nature-based solutions in agricultural landscapes for reducing tradeoffs between food production, climate change, and conservation objectives
Authors: Miralles-Wilhelm Fernando
Published: December 2023

This paper presents a synthesis of evidence and implementation gaps in the application of nature-based solutions (NbS) in agricultural landscapes that contribute to reduce trade-offs between food production, climate change and conservation objectives. The literature and data surveyed relies primarily in peer-reviewed sources and is organized around an overview of NbS science and applications in agricultural landscapes in major biomes. To date, the focus of NbS applications in food production has been predominantly for carbon sequestration, water quality, and disaster-risk management objectives while documented examples of NbS benefits in agricultural production are sparse. Conservation applications of NbS appear to show evidence of effectiveness across multiple objectives in biodiversity, land, soil and water. Evidence and analysis of NbS to meet climate change mitigation targets has surged in recent years driven by global community efforts. Overall, considerable scientific work remains to refine and reduce the uncertainty of NbS benefit estimates across production, climate and conservation objectives, and resilience implications. However, delaying implementation of NbS in agricultural landscapes would likely increase the costs to meet agricultural production, climate, conservation and other societally beneficial goals, while degrading the capacity of natural systems to continue to provide these and other ecosystem services.
Quantification of run-of-river hydropower potential in the Upper Indus basin under climate change
Authors: Dhaubanjar Sanita , Lutz Arthur F. , Smolenaars Wouter Julius , Khanal Sonu , Jamil Muhammad Khalid , Biemans Hester , Ludwig Fulco , Shrestha Arun Bhakta , Immerzeel Walter W.
Published: December 2023

Introduction

Despite ambitious plans to quadruple hydropower generation in the Indus basin, a quantitative assessment of the impact of climate change on hydropower availability in the basin is missing. To address this gap, we combine downscaled CMIP6 projections with the Hydropower Potential Exploration (HyPE) model to quantify future hydropower potential available in the upper Indus basin.

Methods

HyPE uses a spatial cost-minimization framework to evaluate four classes of hydropower potential, namely theoretical, technical, financial and sustainable, considering various constraints on the siting and sizing of two run-of-river hydropower plant configurations.

Results

Under future discharge projections, all classes of potential increase while subbasin changes align with the spatial patterns projected in hydro-climatology. Theoretical potential changes by 3.9–56 %, technical potential by −2.3–46.8 %, financial potential by −8.8–50.4 % and sustainable potential by −6.1–49.7 %. A small decline is observed in the northwestern subbasins where increase in potential is lower than in the southeast. In contrast, with increasing variability in the Indian Summer Monsoon in the future, the southeastern subbasins have the strongest increase in sustainable potential accompanied by higher increase in plant size, decrease in costs and higher variability. The southeastern Satluj subbasin is the hotspot where sustainable potential has the highest increase of up to 145 %. The northwestern Kabul subbasin has the highest decrease of up to −27 %. The Swat subbasin has the lowest variability in sustainable potential while the Jhelum and Indus main subbasins remain the subbasins with the cheapest potential into the future. The performance of future sustainable portfolios differ from the performance of historical portfolios by −11.1–39.9 %.

Discussion

Hence, considering future climate in the present-day planning of hydropower will lead to improved performance under a majority of scenarios. The sufficiency of hydropower potential to fulfill energy security depends on future population growth. Energy availability is projected to decline in the northwest as population increases faster than hydropower potential. The per capita sustainable potential In the Kabul subbasin reduces to a third of the historical value. A socio-hydrological approach is necessary to address the complexity of achieving sustainable and equitable hydropower development in the Indus basin under such spatial mismatch between hydropower availability and energy demand in a resource-limited world.
Crane-powered pectus excavatum repair: the NeoPectus surgery
Authors: Park Hyung Joo , Rim Gongmin , Yoon Seung Keun
Published: December 2023

Introduction

The conventional technique for pectus excavatum repair was pushing up the depressed chest wall by turning over the convexity of the bent pectus bar. We developed a new concept in which a total crane lift was used as the source of power to elevate the depressed sternum without using pectus bar leverage. This study aims to verify the efficacy of exclusively crane-powered pectus excavatum repair in recent years.

Methods

Among the 3622 pectus deformity repairs since 1999, 691 cases repaired with the total crane power between 2017 and 2022 were enrolled. The mean age was 12.0 years (3–45). The operative technique involved wire/screw crane elevation of the sternum, the entire chest wall remodeling with the cross or parallel bars, the bridge/claw bar fixations, and other adjunctive techniques. Outcome analysis included morphological variations, patterns of pectus bar placement, and complication rates.

Results

The crane technique and pectoscopy (100%) were used. The bar placements were parallel (22.0%) and cross-bar (47.5%). The bar fixations were the claw fixators for a single bar (30.5%) and the bridge plates for multiple bars (69.5%). The flare-buster and magic strings were liberally used. No serious complications or catastrophic events occurred, but minor complications occurred in 16.9%: pneumothorax in 7.4% (51), pleural effusion in 1.6% (11), and wound problems in 0.4% (3). There was no case of bar displacement.

Discussion

The crane-powered pectus excavatum repair showed excellent results with minimal complications and no bar displacement. This innovative approach, part of the NeoPectus surgery, represents a significant advancement in correcting pectus excavatum deformities by utilizing a crane machine to elevate the chest wall.
Dynamic Queries through Augmented Reality for Intelligent Video Systems
Authors: Montes-Martínez Josue-Rafael , Jiménez-Hernández Hugo , Herrera-Navarro Ana-Marcela , Díaz-Jiménez Luis-Antonio , Perez-Ramos Jorge-Luis , Solano-Vargas Julio-César
Published: December 2023

Artificial vision system applications have generated significant interest as they allow information to be obtained through one or several of the cameras that can be found in daily life in many places, such as parks, avenues, squares, houses, etc. When the aim is to obtain information from large areas, it can become complicated if it is necessary to track an object of interest, such as people or vehicles, due to the vision space that a single camera can cover; this opens the way to distributed zone monitoring systems made up of a set of cameras that aim to cover a larger area. Distributed zone monitoring systems add great versatility, becoming more complex in terms of the complexity of information analysis, communication, interoperability, and heterogeneity in the interpretation of information. In the literature, the development of distributed schemes has focused on representing data communication and sharing challenges. Currently, there are no specific criteria for information exchange and analysis in a distributed system; hence, different models and architectures have been proposed. In this work, the authors present a framework to provide homogeneity in a distributed monitoring system. The information is obtained from different cameras, where a global reference system is defined for generated trajectories, which are mapped independently of the model used to obtain the dynamics of the movement of people within the vision area of a distributed system, thus allowing for its use in works where there is a large amount of information from heterogeneous sources. Furthermore, we propose a novel similarity metric that allows for information queries from heterogeneous sources. Finally, to evaluate the proposed performance, the authors developed several distributed query applications in an augmented reality system based on realistic environments and historical data retrieval using a client–server model.
Video Games and the COVID-19 Pandemic: Virtual Worlds as New Playgrounds and Training Spaces
Authors: Somoza Medina Xosé , Somoza Medina Marta
Published: December 2023

The COVID-19 pandemic forced the authorities to take an unprecedented measure in history: the house confinement of millions of people worldwide. Video games, especially open-world video games (OWVGs), became meeting spaces, a digital places to play, chat, learn and socialize due to the context of the health crisis, respecting the rules of social distancing. This article analyses the role of video games and, more specifically, OWVGs, as playgrounds and training spaces during the pandemic. Statistical data and analyses carried out by consulting companies and civil associations show the definitive insertion of these video games in our routine and social relations. The challenge is to take advantage of the skills and abilities that these video games develop within a new framework of individual and community learning. The conclusions of the research show that the virtual worlds of video games are for the new digital society, safe and comfortable meeting spaces, and that since the confinement, these digital places have greatly expanded their reach, previously only limited to the gamer community.
Robustness of Wave–Particle Duality under Unruh Effect
Authors: Barros Pedro H. M. , da Paz Irismar G. , Neto Olimpio P. de Sá , Costa Helder A. S.
Published: December 2023

By considering a uniformly accelerated two-level system in an initial superposition state of a qubit, we investigate the loss of coherence induced by the acceleration. In addition, we investigate the impact of acceleration on the complementarity relation in a quantum interferometric circuit or quantum scattering circuit. We present an alternative approach to exploring acceleration effects through examination of quantum coherence decay and degradation in the interference pattern. Our investigations help to provide understanding of the consequences of decoherence induced by the Unruh effect on the wave–particle duality of a uniformly accelerated qubit.
From Academia to Industry: Criteria for Upscaling Ionic Liquid-Based Thermo-Electrochemical Cells for Large-Scale Applications
Authors: Tiozzo Arianna , Bertinetti Andrea , Tommasi Alessio , Nicol Giovanna , Rocca Riccardo , Nakamae Sawako , Torres Bautista Blanca E. , Campagna Zignani Sabrina , Laux Edith , Fantini Sebastien , Sgroi Mauro Francesco
Published: December 2023

Thermo-electrochemical cells (or thermocells) represent a promising technology to convert waste heat energy into electrical energy, generating power with minimal material consumption and a limited carbon footprint. Recently, the adoption of ionic liquids has pushed both the operational temperature range and the power output of thermocells. This research discusses the design challenges and the key performance limitations that need to be addressed to deploy the thermocells in real-world applications. For this purpose, a unique up-scaled design of a thermocell is proposed, in which the materials are selected according to the techno-economic standpoint. Specifically, the electrolyte is composed of EMI-TFSI ionic liquid supplemented by [Co(ppy)]3+/2+ redox couples characterized by a positive Seebeck coefficient (1.5 mV/K), while the electrodes consist of carbon-based materials characterized by a high surface area. Such electrodes, adopted to increase the rate of the electrode reactions, lead to a thermoelectric performance one order of magnitude greater than the Pt electrode-based counterpart. However, the practical applications of thermocells are still limited by the low power density and low voltage that can be generated.
Numerical Analysis of the Effect of Floor Depression on the Extent of Thermal Interaction with the Ground and Energy Management Using a Vegetable Cold Store as an Example
Authors: Sokołowski Paweł , Jakubowski Tomasz , Nawalany Grzegorz , Atilgan Atilgan , Syrotyuk Serhiy
Published: December 2023

The thermal interaction between cooling facilities and the ground is most often discussed in terms of the appropriate insulation of building partitions. Unfortunately, there is little information about the potential of using ground thermal accumulation to support the shaping of the microclimate in cooling facilities by embedding them in the ground. This problem is particularly important in the context of striving to reduce the energy demand of buildings. The article discusses a new scientific problem related to the effect of vegetable cold storage floors being recessed into the ground on the surrounding land’s impact range and on its energy management. Validation of the numerical model was performed based on actual year-round field surveys. These surveys were conducted in a free-standing vegetable cold storage facility located in southern Poland. The results of the study allowed us to determine the contribution of the land to the energy balance of the cold storage. A floor recessed into the ground doubled the ground’s contribution to the energy balance. The most important research results showed that the range of thermal impact on the surrounding ground also increased by 2.0 m more than that of a building with the floor located at ground level. An evaluation of the heat flow between the cold storage and the ground in the cases analyzed was also carried out. The analysis of the ground heat exchange balance on an annual basis showed high energy gains of 2055 kWh. The total energy demand for cooling was 1723 kWh, while it was 1204 kWh for heating. The results of the analysis of the heat exchange intensity between the indoor air and the ground showed that the ground contribution accounted for 16.6% of the total energy balance of the cold storage. The highest energy gains from the ground were found in October and amounted to 478 kWh. Due to the summer shutdown, there was an intense heat flow to the ground in July, which amounted to 588 kWh.
Enhanced Efficiency on ANPC-DAB through Adaptive Model Predictive Control
Authors: Nardoto Adriano , Encarnação Lucas , Santos Walbermark , Amorim Arthur , Fiorotti Rodrigo , Molinero David , Bueno Emilio
Published: December 2023

This work studies the DC-DC conversion stage in solid-state transformers (SST). The traditional two- or three-level dual active bridge (DAB) topology faces limitations in microgrid interconnection due to power and voltage limitations. For this reason, the use of multilevel topologies such as active neutral point clamped (ANPC) is a promising alternative. Additionally, the efficiency of the SSTs is a recurring concern, and reducing losses in the DC-DC stage is a subject to be studied. In this context, this work presents a new control technique based on an adaptive model- based predictive control (AMPC) to select the modulation technique of an ANPC-DAB DC-DC converter aimed at reducing losses and increasing efficiency. The single-phase shift (SPS), triangular, and trapezoidal modulation techniques are used according to the converter output power with the aim of maximizing the number of soft-switching points per cycle. The performance of the proposed control technique is demonstrated through real-time simulation and a reduced-scale experimental setup. The findings indicate the effectiveness of the AMPC control technique in mitigating voltage source perturbations. This technique has low output impedance and is robust to converter parameter variations. Prototyping tests revealed that, in steady-state, the AMPC significantly improves converter efficiency without compromising dynamic performance. Despite its advantages, the computational cost of AMPC is not significantly higher than that of traditional model predictive control (MPC), allowing for the allocation of time to other applications.
Increasing the Efficiency of Diagnostics in the Brush-Commutator Assembly of a Direct Current Electric Motor
Authors: Filina Olga A. , Martyushev Nikita V. , Malozyomov Boris V. , Tynchenko Vadim Sergeevich , Kukartsev Viktor Alekseevich , Bashmur Kirill Aleksandrovich , Pavlov Pavel P. , Panfilova Tatyana Aleksandrovna
Published: December 2023

Increasing the productivity and reliability of mining infrastructure facilities is an important task in achieving future goals. Mining dump trucks are an important part of coal mine infrastructure. In this article, to determine the reliability indicators in a brush–commutator unit and the residual life of electric motor brushes, a mathematical model for processing statistical data has been developed, which allows for the classification of types of failures and, unlike existing models, the determination of the life of the brushes according to the maximum extent of their wear. A method for predicting the residual life of an electric brush in a DC electric motor is presented, which contains a list of controlled reliability indicators, included a mathematical model. The described model improves the accuracy of the prediction and detection of DC motor failures. The derivation of the general formula for calculating the residual life of electric brushes is given. Based on the proposed mathematical model, we studied and calculated the reliability of the brush–commutator unit, the minimum height of the brush during operation, the average rate of its wear, the standard deviation and the mathematical expectation of brush wear. A nomogram of the failure-free operation time of the brush–commutator unit in a DC electric motor was modeled using the height of the brush during operation. Output parameters after the implementation of this monitoring system include the reliability of the electric motor operation. At the same time, diagnostic characteristics are improved twofold, and the residual life of the brush-switching unit is increased by 28–30%.
Antimicrobial Activity of N,N-Diethyldithiocarbamate against Ureaplasma parvum and Ureaplasma urealyticum
Authors: Biernat-Sudolska Małgorzata , Rojek-Zakrzewska Danuta , Drożdż Kamil , Bilska-Wilkosz Anna
Published: December 2023

Ureaplasma species (Ureaplasma spp.) are commonly found as commensals in the human urogenital tracts, although their overgrowth can lead to infection in the urogenital tract and at distal sites. Furthermore, ureaplasmas lack a cell wall and do not synthesize folic acid, which causes all β-lactam and glycopeptide antibiotics, and sulfonamides and diaminopyrimidines, to be of no value. The antibiotics used in therapy belong to the fluoroquinolone, tetracycline, chloramphenicol and macrolide classes. However, the growing incidence of antibiotic-resistant Ureaplasma spp. in the population becomes a problem. Thus, there is a need to search for new drugs effective against these bacteria. Since 1951, the FDA-approved, well-tolerated, inexpensive, orally administered drug disulfiram (DSF) has been used in the treatment of chronic alcoholism, but recently, its antimicrobial effects have been demonstrated. The main biological metabolite of DSF, i.e., N,N-diethyldithiocarbamate (DDC), is generally believed to be responsible for most of the observed pharmacological effects of DSF. In the presented studies, the effect of DDC at concentrations of 2 µg/mL, 20 µg/mL and 200 µg/mL on the growth and survival of Ureaplasma urealyticum and Ureaplasma parvum was tested for the first time. The results indicated that all the used DDC concentrations showed both bacteriostatic and bactericidal activity against both tested strains.
A Phase II Exploratory Study to Identify Biomarkers Predictive of Clinical Response to Regorafenib in Patients with Metastatic Colorectal Cancer Who Have Failed First-Line Therapy
Authors: Gambaro Karen , Marques Maud , McNamara Suzan , Couetoux du Tertre Mathilde , Hoffert Cyrla , Srivastava Archana , Schab Anna , Alcindor Thierry , Langleben Adrian , Sideris Lucas , Abdelsalam Mahmoud , Tehfe Mustapha , Couture Felix , Batist Gerald , Kavan Petr
Published: December 2023

Single-agent regorafenib is approved in Canada for metastatic colorectal cancer (mCRC) patients who have failed previous lines of therapy. Identifying prognostic biomarkers is key to optimizing therapeutic strategies for these patients. In this clinical study (NCT01949194), we evaluated the safety and efficacy of single-agent regorafenib as a second-line therapy for mCRC patients who received it after failing first-line therapy with an oxaliplatin or irinotecan regimen with or without bevacizumab. Using various omics approaches, we also investigated putative biomarkers of response and resistance to regorafenib in metastatic lesions and blood samples in the same cohort. Overall, the safety profile of regorafenib seemed similar to the CORRECT trial, where regorafenib was administered as ≥ 2 lines of therapy. While the mutational landscape showed typical mutation rates for the top five driver genes (APC, KRAS, BRAF, PIK3CA, and TP53), KRAS mutations were enriched in intrinsically resistant lesions. Additional exploration of genomic-phenotype associations revealed several biomarker candidates linked to unfavorable prognoses in patients with mCRC using various approaches, including pathway analysis, cfDNA profiling, and copy number analysis. However, further research endeavors are necessary to validate the potential utility of these promising genes in understanding patients’ responses to regorafenib treatment.
The Localization of Cell Wall Components in the Quadrifids of Whole-Mount Immunolabeled Utricularia dichotoma Traps
Authors: Płachno Bartosz J. , Kapusta Małgorzata
Published: December 2023

Utricularia (bladderworts) are carnivorous plants. They produce small hollow vesicles, which function as suction traps that work underwater and capture fine organisms. Inside the traps, there are numerous glandular trichomes (quadrifids), which take part in the secretion of digestive enzymes, the resorption of released nutrients, and likely the pumping out of water. Due to the extreme specialization of quadrifids, they are an interesting model for studying the cell walls. This aim of the study was to fill in the gap in the literature concerning the immunocytochemistry of quadrifids in the major cell wall polysaccharides and glycoproteins. To do this, the localization of the cell wall components in the quadrifids was performed using whole-mount immunolabeled Utricularia traps. It was observed that only parts (arms) of the terminal cells had enough discontinuous cuticle to be permeable to antibodies. There were different patterns of the cell wall components in the arms of the terminal cells of the quadrifids. The cell walls of the arms were especially rich in low-methyl-esterified homogalacturonan. Moreover, various arabinogalactan proteins also occurred. Cell walls in glandular cells of quadrifids were rich in low-methyl-esterified homogalacturonan; in contrast, in the aquatic carnivorous plant Aldrovanda vesiculosa, cell walls in the glandular cells of digestive glands were poor in low-methyl-esterified homogalacturonan. Arabinogalactan proteins were found in the cell walls of trap gland cells in all studied carnivorous plants: Utricularia, and members of Droseraceae and Drosophyllaceae.
Stochastic Simulation of Construction Methods for Multi-purpose Utility Tunnels
Authors: Jorjam Shayan , Mawlana Mohammed , Hammad Amin
Published: December 2023

The traditional method of installing underground utilities (e.g., water, sewer, gas pipes, electrical cables) by burying them under roads has been used for decades. However, the repeated excavations related to this method cause problems, such as traffic congestion and business disruption, which can significantly increase financial and social costs. Multi-purpose Utility Tunnels (MUTs) are a good alternative for buried utilities. Although the initial cost of MUTs is higher than that of the traditional method, social cost savings make them more feasible, especially in dense urban areas. Different factors, such as the specifications of utilities, the location of the MUTs, and the construction method, should be investigated to determine if MUTs can be an economical and practical alternative. The construction method is one of the most important factors to assess to have a successful MUT project and reduce its impact on the surrounding area. Simulation can be used to investigate the different construction methods of MUTs. In this paper, two Stochastic Discrete Event Simulation models depicting two MUT construction methods (i.e., microtunneling and cut-and-cover) are developed to analyze the duration and cost of the MUT projects. Also, 4D simulation models of these methods are developed for constructability assessment of these projects.
In Silico Analysis of USP7 Inhibitors Based on Building QSAR Models and Fragment Design for Screening Marine Compound Libraries
Authors: Tan Huiting , Li Chenying , Lai Tianli , Luo Lianxiang
Published: December 2023

USP7 is highly expressed in a variety of tumors and is thought to play a major role in cancer development. However, there are no drugs available to target USP7, so there is a need to develop new USP7 inhibitors. In this study, AutoQSAR, multiple linear regression, and Naive Bayesian models were constructed using 543 compounds and used to analyze marine compounds. After selecting 240 small molecules for molecular docking with Maestro, MOE, and GOLD, better small molecules than the positive compound P217564 were screened. The molecular structure of “1, 2-dibromobenzene” was optimized to improve the binding effect of the protein, and 10 optimized compounds in ADMET performed well during the screening process. To study the dynamic combination of protein–ligand effect consistency with static molecular docking, 100ns molecular dynamics simulations of candidate compound 1008-1, reference compound P217564, and negative-positive GNE2917 were conducted. The results of molecular docking and molecular dynamics simulation analysis showed that compound 1008-1 maintained a stable conformation with the target protein. Thus, the comprehensive analysis suggests that compound 1008-1 could provide new possibilities for USP7 covalent inhibitor candidates.