bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Long-Term Mortality in TAVI Patients Using Machine Learning Techniques
Authors: Penso Marco , Pepi Mauro , Fusini Laura , Muratori Manuela , Cefalù Claudia , Mantegazza Valentina , Gripari Paola , Ali Sarah Ghulam , Fabbiocchi Franco , Bartorelli Antonio L. , Caiani Enrico G. , Tamborini Gloria
Published: April 2021

Background: Whereas transcatheter aortic valve implantation (TAVI) has become the gold standard for aortic valve stenosis treatment in high-risk patients, it has recently been extended to include intermediate risk patients. However, the mortality rate at 5 years is still elevated. The aim of the present study was to develop a novel machine learning (ML) approach able to identify the best predictors of 5-year mortality after TAVI among several clinical and echocardiographic variables, which may improve the long-term prognosis. Methods: We retrospectively enrolled 471 patients undergoing TAVI. More than 80 pre-TAVI variables were collected and analyzed through different feature selection processes, which allowed for the identification of several variables with the highest predictive value of mortality. Different ML models were compared. Results: Multilayer perceptron resulted in the best performance in predicting mortality at 5 years after TAVI, with an area under the curve, positive predictive value, and sensitivity of 0.79, 0.73, and 0.71, respectively. Conclusions: We presented an ML approach for the assessment of risk factors for long-term mortality after TAVI to improve clinical prognosis. Fourteen potential predictors were identified with the organic mitral regurgitation (myxomatous or calcific degeneration of the leaflets and/or annulus) which showed the highest impact on 5 years mortality.
Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans
Authors: Saito Junichi , Kojima Tomoyuki , Tanifuji Shota , Kato Yuko , Oka Sayuki , Ichikawa Yasuhiro , Miyagi Etsuko , Tachibana Tsuyoshi , Asou Toshihide , Yokoyama Utako
Published: April 2021

The ductus arteriosus (DA) immediately starts closing after birth. This dynamic process involves DA-specific properties, including highly differentiated smooth muscle, sparse elastic fibers, and intimal thickening (IT). Although several studies have demonstrated DA-specific gene expressions using animal tissues and human fetuses, the transcriptional profiles of the closing DA and the patent DA remain largely unknown. We performed transcriptome analysis using four human DA samples. The three closing DA samples exhibited typical DA morphology, but the patent DA exhibited aorta-like elastic lamellae and poorly formed IT. A cluster analysis revealed that samples were clearly divided into two major clusters, the closing DA and patent DA clusters, and showed distinct gene expression profiles in IT and the tunica media of the closing DA samples. Cardiac neural crest-related genes such as JAG1 were highly expressed in the tunica media and IT of the closing DA samples compared to the patent DA sample. Abundant protein expressions of jagged 1 and the differentiated smooth muscle marker calponin were observed in the closing DA samples but not in the patent DA sample. Second heart field-related genes such as ISL1 were enriched in the patent DA sample. These data indicate that the patent DA may have different cell lineages compared to the closing DA.
Soil Classification and Site Variability Analysis Based on CPT—A Case Study in the Yellow River Subaquatic Delta, China
Authors: Yang Zhongnian , Liu Xuesen , Guo Lei , Cui Yuxue , Su Xiuting , Ling Xianzhang
Published: April 2021

The Yellow River Delta is located at the junction of the Yellow River and Bohai. The impact function from the river and the dynamics of the ocean tides make the soil composition and distribution in this area substantially complicated. In order to test the distribution and variation of the soil layers in the Yellow River Delta, the soil layers in the test area were classified and the variation was calculated using the cone penetration test (CPT). The following conclusions were drawn: (1) the soil in the measured area is mainly composed of sensitive fine-grained soil, accounting for about 70% of all soil types, and the content of sensitive fine-grained soil in the far-sea position is higher than that in the offshore position in the direction perpendicular to the coastline. (2) It has a high vertical variability index (VVI) at the near-shore location, above 45%, and a low vertical variability at the far coast, generally below 20%. (3) The horizontal variability index (HVI) changes significantly near the coast, and it remains below 45% in the test area.
Unfolded Protein Response and Scaffold Independent Pheromone MAP Kinase Signaling Control Verticillium dahliae Growth, Development, and Plant Pathogenesis
Authors: Starke Jessica , Harting Rebekka , Maurus Isabel , Leonard Miriam , Bremenkamp Rica , Heimel Kai , Kronstad James W. , Braus Gerhard H.
Published: April 2021

Differentiation, growth, and virulence of the vascular plant pathogen Verticillium dahliae depend on a network of interconnected cellular signaling cascades. The transcription factor Hac1 of the endoplasmic reticulum-associated unfolded protein response (UPR) is required for initial root colonization, fungal growth, and vascular propagation by conidiation. Hac1 is essential for the formation of microsclerotia as long-time survival resting structures in the field. Single endoplasmic reticulum-associated enzymes for linoleic acid production as precursors for oxylipin signal molecules support fungal growth but not pathogenicity. Microsclerotia development, growth, and virulence further require the pheromone response mitogen-activated protein kinase (MAPK) pathway, but without the Ham5 scaffold function. The MAPK phosphatase Rok1 limits resting structure development of V.dahliae, but promotes growth, conidiation, and virulence. The interplay between UPR and MAPK signaling cascades includes several potential targets for fungal growth control for supporting disease management of the vascular pathogen V.dahliae.
The Role of TQMk in Increasing the Effectiveness of E-Marketing within the Jordanian Telecommunication Sector
Authors: Al-Zyoud Mohammad Fahmi , Al-Mu’ani Lu’ay , Alsoud Malek , Alsoud Anas
Published: April 2021

The current study focused on examining the role of TQMk (Total Quality Marketing) in increasing the effectiveness of e-marketing within Jordanian telecommunication sector; TQMk included variables of service quality, market orientation and the customer-focused approach. A quantitative approach was adopted through utilizing a questionnaire, which was distributed to 18 marketing and project managers within Jordanian telecommunication organizations (Zain, Umniah and Orange). Results of the study indicated that TQMk can have an influence in increasing the effectiveness and efficiency of e-marketing solutions within the organization and mainly within the social marketing and electronic marketing departments, through developing the variable of the customer-focused approach, which has the deepest influence on e-marketing approach’s effectiveness; it was followed by an influence of service quality, and the least influential factor was market orientation. The study recommended focusing on clients within the targeted markets through different aspects, including price, new products acceptance, customer behavior and purchase decision motivators.
Failure Load and Fatigue Behavior of Monolithic Translucent Zirconia, PICN and Rapid-Layer Posterior Single Crowns on Zirconia Implants
Authors: Spitznagel Frank A. , Röhrig Sara , Langner Robert , Gierthmuehlen Petra C.
Published: April 2021

This laboratory study aimed to evaluate the thermo-mechanical fatigue behavior and failure modes of monolithic and rapid-layer posterior single-crowns (SCs) supported by zirconia implants. Methods: 120 all-ceramic crowns supported by one-piece zirconia implants (ceramic.implant; vitaclinical) were divided into five groups (n = 24 each): Group Z-HT: 3Y-TZP monolithic-zirconia (Vita-YZ-HT); Group Z-ST: 4Y-TZP monolithic-zirconia (Vita-YZ-ST); Z-XT: 5Y-TZP monolithic-zirconia (Vita-YZ-XT); Group E: monolithic-polymer-infiltrated ceramic network (PICN,Vita-Enamic); Group RL (rapid layer): PICN-“table-top” (Vita-Enamic), 3Y-TZP-framework (Vita-YZ-HT). Half of the specimens of each group (n = 12) were exposed to fatigue with cyclic mechanical loading (F = 198N, 1.2-million cycles) and simultaneous thermocycling (5–55 °C). Single-load-to-failure testing (Z010, Zwick) was performed for all specimens without/with fatigue application. Data analysis was performed using ANOVA, Tukey’s post-hoc test, two-sample t-test and Bonferroni correction (p < 0.05). Results: All specimens survived fatigue exposure. Significant differences in failure loads were detected among groups (p ≤ 0.004). Materials Z-HT and Z-ST showed the highest failure loads followed by Z-XT, RL and E. The influence of fatigue was only significant for material RL. Conclusions: All types of tested materials exceeded clinically acceptable failure load values higher than 900N and can be recommended for clinical use. Z-HT and Z-ST appear to be highly reliable towards fatigue. Rapid-layer design of PICN and YZ-HT might be an interesting treatment concept for posterior implant SCs.
A Micro-Computed Tomography Comparison of the Porosity in Additively Fabricated CuCr1 Alloy Parts Using Virgin and Surface-Modified Powders
Authors: Sinico Mirko , Jadhav Suraj Dinkar , Witvrouw Ann , Vanmeensel Kim , Dewulf Wim
Published: April 2021

Recently, the use of novel CuCr1 surface-modified powder for reliable laser powder-bed fusion (LPBF) manufacturing has been proposed, enabling a broader LPBF processing window and longer powder storage life. Nevertheless, virgin CuCr1 powder is also LPBF processable, on the condition that a high-energy density is employed. In this work, we compare two dense specimens produced from virgin and surface-modified CuCr1 powder. Furthermore, a third sample fabricated from surface-modified powder is characterized to understand an abnormal porosity content initially detected through Archimedes testing. Utilizing high-resolution micro-CT scans, the nature of the defects present in the different samples is revealed. Pores are analyzed in terms of size, morphology and spatial distribution. The micro-CT data reveal that the virgin CuCr1 dense specimen displays keyhole pores plus pit cavities spanning multiple layer thicknesses. On the other hand, the sample fabricated with the surface-modified CuCr1 powder mainly contains small and spherical equi-distributed metallurgical defects. Finally, the CT analysis of the third specimen reveals the presence of a W contamination, favoring lack-of-fusion pores between subsequent LPBF layers. The LPBF melting mode (keyhole or conductive), the properties of the material, and the potential presence of contaminants are connected to the different porosity types and discussed.
Electrical Properties of Textiles Treated with Graphene Oxide Suspension
Authors: Nikolaev Danil Valeriyevich , Evseev Zakhar Ivanovich , Smagulova Svetlana Afanasyevna , Antonova Irina Veniaminovna
Published: April 2021

Two-dimensional nanomaterials such as graphene can provide various functional properties to textiles, which have great potential in sportswear, healthcare etc. In this study, the properties of nylon and cotton-based electronic textiles coated with reduced graphene oxide are investigated. After reduction of graphene oxide coating in hydrazine vapor, e-textiles with a resistance of ~350 Ω/sq for nylon, and ~1 kΩ/sq for cotton were obtained. Cyclic mechanical bending tests of samples showed that the resistance increases during bending up to 10–20%. The use of bovine serum albumin as an adhesive layer improved the wash stability for samples with nylon up to 40 washing cycles. The use of BF-6 glue as a protective layer reduced changes in resistance during bending, and improved wash stability of cotton samples. It was shown that the resistance of the obtained samples is sensitive to changes in temperature and humidity. In addition, obtained e-textiles attached to a person’s wrist were able to measure heart rate. Thus, the obtained electronic textiles based on cotton and nylon coated with reduced graphene oxide demonstrates good characteristics for use as sensors for monitoring vital signs.
Ultraviolet Luminescence of ZnO Whiskers, Nanowalls, Multipods, and Ceramics as Potential Materials for Fast Scintillators
Authors: Venevtsev Ivan D. , Tarasov Andrey P. , Muslimov Arsen E. , Gorokhova Elena I. , Zadorozhnaya Ludmila A. , Rodnyi Piotr A. , Kanevsky Vladimir M.
Published: April 2021

The presented work is dedicated to the study and comparison of scintillating properties of zinc oxide samples prepared in different morphologies: whiskers, nanowalls, multipods, and ceramics. It was shown that total transmittance, photo- and radioluminescence spectra, and radioluminescence kinetics can vary significantly depending on sample structure and preparation conditions. The highest total transmittance was registered for ZnO ceramics (>50% at 0.5 mm thickness). Differences in the transmittance of whiskers, nanowalls, and multipods can be attributed to their shape and thickness which affects the amount of light refraction and scattering. The study of radioluminescence demonstrated that all samples, except undoped ceramics and air annealed whiskers, have predominantly fast luminescence with a decay time <1 ns. High transmittance of ceramics opens the way for their use in the registration of high energy X-ray and gamma radiation, where a large volume of scintillators is required. In cases, where large scintillator thickness is not a necessity, one may prefer to use other ZnO structures, such as ensembles of whiskers and nanowalls. Studies of near-band-edge luminescence components at low temperatures showed that the structure is quite similar in all samples except Ga doped ceramics.
3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine
Authors: Merk Markus , Chirikian Orlando , Adlhart Christian
Published: April 2021

Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the present study, we marry the commonly used two-dimensional (2D) technique of electrospinning and a self-assembly process to construct easily reproducible, highly porous, three-dimensional (3D) nanofiber scaffolds for various tissue engineering applications. Specimens from biologically relevant polymers polycaprolactone (PCL) and gelatin were chemically cross-linked using the naturally occurring cross-linker genipin. Potential cytotoxic effects of the scaffolds were analyzed by culturing human dermal fibroblasts (HDF) up to 23 days. The 3D PCL/gelatin/genipin scaffolds produced here resemble the complex nanofibrous architecture found in naturally occurring extracellular matrix (ECM) and exhibit physiologically relevant mechanical properties as well as excellent cell cytocompatibility. Samples cross-linked with 0.5% genipin demonstrated the highest metabolic activity and proliferation rates for HDF. Scanning electron microscopy (SEM) images indicated excellent cell adhesion and the characteristic morphological features of fibroblasts in all tested samples. The three-dimensional (3D) PCL/gelatin/genipin scaffolds produced here show great potential for various 3D tissue-engineering applications such as ex vivo cell culturing platforms, wound healing, or tissue replacement.
Research on the Grinding Energy Density in a Jet Mill
Authors: Urbaniak Dariusz , Otwinowski Henryk , Wyleciał Tomasz , Zhukov Vladimir Pavlovich , Barochkin Aleksei Yevgenyevich , Boryca Jarosław
Published: April 2021

Raw materials are used in many industrial technologies. The raw material frequently has to be prepared as an intermediate with an appropriate particle size distribution, which requires the use of grinding. In grinding processes, energy consumption is a very important profitability criterion for the applied particular size reduction technology. The paper describes the comminution process that takes place in the jet mill using a modified form of the thermodynamic theory of grinding. In this theory, new material characteristics have been added: the surface and volumetric density of grinding energy. The thermodynamic theory is a combination of the classical Kick’s theory and the modified form of Rittinger’s theory. The tested physical magnitudes are a measure of the energy consumption of the grinding process. They describe the energy that must be provided in the grinding process to overcome interactions between particles related to the volume and surface of the material. Knowledge of these magnitudes is necessary to model thermomechanical phenomena in the solid state. The paper presents the results of research on comminution in a jet mill, on the basis of which the values of the tested material magnitudes were determined. It is graphically shown how the values of the tested magnitudes depend on the grain size of the ground samples.
Thermal and Mechanical Studies of Perlite Concrete Casing for Chimneys in Residential Buildings
Authors: Drozdzol Krzysztof
Published: April 2021

Chimneys are structures designed to convey exhaust gases from heating devices to the outside of buildings. The materials from which they are made have a great impact on their fire safety, as well as on the safety of the whole building. As current trends in the construction industry are moving towards improving the environmental impact and fire safety, changes to building materials are constantly being introduced. This also applies to the development of chimney technology, as there is still a recognised need for new solutions when it comes to materials used in the production of chimney systems. This article presents the findings of tests carried out on a chimney made from innovative perlite concrete blocks. Four different perlite concrete blocks that differed in bulk densities were analysed. The obtained results were then compared with widely used leca (lightweight expanded clay aggregate) concrete blocks. The test results confirmed high insulation properties of the perlite concrete block, from which the innovative chimney casing was made. The fire safety level was maintained even in high temperatures that occur during soot fire (1000 °C). These properties were retained despite there being no additional insulation of the flue duct. Even though the thermal load decreased the compressive strength of the chimney blocks, they still displayed sufficient average strength of 4.03 MPa. Additionally, the test results confirmed the possibility of recovering heat from the chimney with the efficiency of 23–30%, which constitutes a considerable increase compared to chimneys made from leca concrete blocks.
Mechanical and Fracture Parameters of Ultra-High Performance Fiber Reinforcement Concrete Cured via Steam and Water: Optimization of Binder Content
Authors: Mala Avan Ahmed , Sherwani Aryan Far H. , Younis Khaleel H. , Faraj Rabar H. , Mosavi Amir
Published: April 2021

An investigational study is conducted to examine the effects of different amounts of binders and curing methods on the mechanical behavior and ductility of Ultra-High Performance Fiber Reinforced Concretes (UHPFRCs) that contain 2% of Micro Steel Fiber (MSF). The aim is to find an optimum binder content for the UHPFRC mixes. The same water-to-binder ratio (w/b) of 0.12 was used for both water curing (WC) and steam curing (SC). Based on the curing methods, two series of eight mixes of UHPFRCs containing different binder contents ranging from 850 to 1200 kg/m3 with an increment of 50 kg/m3 were produced. Mechanical properties such as compressive strength, splitting tensile strength, static elastic module, flexural tensile strength and the ductility behavior were investigated. This study revealed that the mixture of 1150 kg/m3 binder content exhibited the highest values of the experimental results such as a compressive strength greater than 190 MPa, a splitting tensile strength greater than 12.5 MPa, and a modulus of elasticity higher than 45 GPa. The results also show that all of the improvements began to slightly decrease at 1200 kg/m3 of the binder content. On the other hand, it was concluded that SC resulted in higher mechanical performance and ductility behavior than WC.
ROM-Based Inexact Subdivision Methods for PDE-Constrained Multiobjective Optimization
Authors: Banholzer Stefan , Gebken Bennet , Reichle Lena , Volkwein Stefan
Published: April 2021

The goal in multiobjective optimization is to determine the so-called Pareto set. Our optimization problem is governed by a parameter-dependent semi-linear elliptic partial differential equation (PDE). To solve it, we use a gradient-based set-oriented numerical method. The numerical solution of the PDE by standard discretization methods usually leads to high computational effort. To overcome this difficulty, reduced-order modeling (ROM) is developed utilizing the reduced basis method. These model simplifications cause inexactness in the gradients. For that reason, an additional descent condition is proposed. Applying a modified subdivision algorithm, numerical experiments illustrate the efficiency of our solution approach.
Optimization of a Spin-Orbit Torque Switching Scheme Based on Micromagnetic Simulations and Reinforcement Learning
Authors: de Orio Roberto L. , Ender Johannes , Fiorentini Simone , Goes Wolfgang , Selberherr Siegfried , Sverdlov Viktor
Published: April 2021

Spin-orbit torque memory is a suitable candidate for next generation nonvolatile magnetoresistive random access memory. It combines high-speed operation with excellent endurance, being particularly promising for application in caches. In this work, a two-current pulse magnetic field-free spin-orbit torque switching scheme is combined with reinforcement learning in order to determine current pulse parameters leading to the fastest magnetization switching for the scheme. Based on micromagnetic simulations, it is shown that the switching probability strongly depends on the configuration of the current pulses for cell operation with sub-nanosecond timing. We demonstrate that the implemented reinforcement learning setup is able to determine an optimal pulse configuration to achieve a switching time in the order of 150 ps, which is 50% shorter than the time obtained with non-optimized pulse parameters. Reinforcement learning is a promising tool to automate and further optimize the switching characteristics of the two-pulse scheme. An analysis of the impact of material parameter variations has shown that deterministic switching can be ensured for all cells within the variation space, provided that the current densities of the applied pulses are properly adjusted.
Design, Fabrication, and Testing of a Novel 3D 3-Fingered Electrothermal Microgripper with Multiple Degrees of Freedom
Authors: Si Guoning , Sun Liangying , Zhang Zhuo , Zhang Xuping
Published: April 2021

This paper presents the design, fabrication, and testing of a novel three-dimensional (3D) three-fingered electrothermal microgripper with multiple degrees of freedom (multi DOFs). Each finger of the microgripper is composed of a V-shaped electrothermal actuator providing one DOF, and a 3D U-shaped electrothermal actuator offering two DOFs in the plane perpendicular to the movement of the V-shaped actuator. As a result, each finger possesses 3D mobilities with three DOFs. Each beam of the actuators is heated externally with the polyimide film. The durability of the polyimide film is tested under different voltages. The static and dynamic properties of the finger are also tested. Experiments show that not only can the microgripper pick and place microobjects, such as micro balls and even highly deformable zebrafish embryos, but can also rotate them in 3D space.
Lignin as a Partial Polyol Replacement in Polyurethane Flexible Foam
Authors: Gondaliya Akash , Nejad Mojgan
Published: April 2021

This study was focused on evaluating the suitability of a wide range of lignins, a natural polymer isolated from different plant sources and chemical extractions, in replacing 20 wt.% of petroleum-based polyol in the formulation of PU flexible foams. The main goal was to investigate the effect of unmodified lignin incorporation on the foam’s structural, mechanical, and thermal properties. The hydroxyl contents of the commercial lignins were measured using phosphorus nuclear magnetic resonance (31P NMR) spectroscopy, molar mass distributions with gel permeation chromatography (GPC), and thermal properties with differential scanning calorimetry (DSC) techniques. The results showed that incorporating 20 wt.% lignin increased tensile, compression, tear propagation strengths, thermal stability, and the support factor of the developed PU flexible foams. Additionally, statistical analysis of the results showed that foam properties such as density and compression force deflection were positively correlated with lignin’s total hydroxyl content. Studying correlations between lignin properties and the performance of the developed lignin-based PU foams showed that lignins with low hydroxyl content, high flexibility (low Tg), and high solubility in the co-polyol are better candidates for partially substituting petroleum-based polyols in the formulation of flexible PU foams intended for the automotive applications.
Fluorescently Labeled Cellulose Nanofibers for Environmental Health and Safety Studies
Authors: Patel Ilabahen , Woodcock Jeremiah , Beams Ryan , Stranick Stephan J. , Nieuwendaal Ryan , Gilman Jeffrey W. , Mulenos Marina R. , Sayes Christie M. , Salari Maryam , DeLoid Glen , Demokritou Philip , Harper Bryan , Harper Stacey , Ong Kimberly J. , Shatkin Jo Anne , Fox Douglas M.
Published: April 2021

An optimal methodology for locating and tracking cellulose nanofibers (CNFs) in vitro and in vivo is crucial to evaluate the environmental health and safety properties of these nanomaterials. Here, we report the use of a new boron-dipyrromethene (BODIPY) reactive fluorescent probe, meso-DichlorotriazineEthyl BODIPY (mDTEB), tailor-made for labeling CNFs used in simulated or in vivo ingestion exposure studies. Time-correlated single photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) was used to confirm covalent attachment and purity of mDTEB-labeled CNFs. The photoluminescence properties of mDTEB-labeled CNFs, characterized using fluorescence spectroscopy, include excellent stability over a wide pH range (pH2 to pH10) and high quantum yield, which provides detection at low (μM) concentrations. FLIM analysis also showed that lignin-like impurities present on the CNF reduce the fluorescence of the mDTEB-labeled CNF, via quenching. Therefore, the chemical composition and the methods of CNF production affect subsequent studies. An in vitro triculture, small intestinal, epithelial model was used to assess the toxicity of ingested mDTEB-labeled CNFs. Zebrafish (Danio rerio) were used to assess in vivo environmental toxicity studies. No cytotoxicity was observed for CNFs, or mDTEB-labeled CNFs, either in the triculture cells or in the zebrafish embryos.
Association between Oral Cancer and Diet: An Update
Authors: Rodríguez-Molinero Jesús , Migueláñez-Medrán Blanca del Carmen , Puente-Gutiérrez Cristina , Delgado-Somolinos Esther , Martín Carreras-Presas Carmen , Fernández-Farhall Javier , López-Sánchez Antonio Francisco
Published: April 2021

Oral cancer, included within head and neck cancer, is the sixth most common malignant neoplasm in the world. The main etiological factors are tobacco and alcohol, although currently, diet is considered an important determinant for its development. Several dietary nutrients have specific mechanisms of action, contributing to both protection against cancer and increasing the risk for development, growth, and spread. Foods such as fruits, vegetables, curcumin, and green tea can reduce the risk of oral cancer, while the so-called pro-inflammatory diet, rich in red meat and fried foods, can enhance the risk of occurrence. Dietary factors with a protective effect show different mechanisms that complement and overlap with antioxidant, anti-inflammatory, anti-angiogenic, and anti-proliferative effects. The main limitation of in vivo studies is the complexity of isolating the effects related to each one of the nutrients and the relationship with other possible etiological mechanisms. On the contrary, in vitro studies allow determining the specific mechanisms of action of some of the dietary compounds. In conclusion, and despite research limitations, the beneficial effects of a diet rich in vegetables and fruits are attributed to different micronutrients that are also found in fish and animal products. These compounds show antioxidant, anti-inflammatory, anti-angiogenic, and anti-proliferative properties that have a preventive role in the development of oral and other types of cancer.
Inadequate Content of Docosahexaenoic Acid (DHA) of Donor Human Milk for Feeding Preterm Infants: A Comparison with Mother’s Own Milk at Different Stages of Lactation
Authors: Castillo Félix , Castillo-Ferrer Félix-Joel , Cordobilla Begoña , Domingo Joan Carles
Published: April 2021

A cross-sectional single-center study was designed to compare the fatty acids profile, particularly docosahexaenoic acid (DHA) levels, between milk banking samples of donor human milk and mother’s own milk (MOM) for feeding preterm infants born before 32 weeks’ gestation. MOM samples from 118 mothers included colostrum (1–7 days after delivery), transitional milk (9–14 days), and mature milk (15–28 days and ≥29 days). In the n-3 polyunsaturated fatty acids (PUFAs) group, the levels of α-linolenic acid (C18:3 n3) and DHA (C22:6 n3) showed opposite trends, whereas α-linolenic acid was higher in donor human milk as compared with MOM, with increasing levels as stages of lactation progressed, DHA levels were significantly lower in donor human milk than in MOM samples, which, in turn, showed decreasing levels along stages of lactation. DHA levels in donor human milk were 53% lower than in colostrum. Therefore, in preterm infants born before 32 weeks’ gestation, the use of pasteurized donor human milk as exclusive feeding or combined with breastfeeding provides an inadequate supply of DHA. Nursing mothers should increase DHA intake through fish consumption or nutritional supplements with high-dose DHA while breastfeeding. Milk banking fortified with DHA would guarantee adequate DHA levels in donor human milk.
Placental Antioxidant Defenses and Autophagy-Related Genes in Maternal Obesity and Gestational Diabetes Mellitus
Authors: Diceglie Cecilia , Anelli Gaia Maria , Martelli Cristina , Serati Anais , Lo Dico Alessia , Lisso Fabrizia , Parisi Francesca , Novielli Chiara , Paleari Renata , Cetin Irene , Ottobrini Luisa , Mandò Chiara
Published: April 2021

Maternal obesity and gestational diabetes mellitus (GDM) are increasing worldwide, representing risk factors for both mother and child short/long-term outcomes. Oxidative stress, lipotoxicity and altered autophagy have already been reported in obesity, but few studies have focused on obese pregnant women with GDM. Antioxidant and macro/chaperone-mediated autophagy (CMA)-related gene expressions were evaluated herein in obese and GDM placentas. A total of 47 women with singleton pregnancies delivered by elective cesarean section were enrolled: 16 normal weight (NW), 18 obese with no comorbidities (OB GDM(–)), 13 obese with GDM (OB GDM(+)). Placental gene expression was assessed by real-time PCR. Antioxidant gene expression (CAT, GPX1, GSS) decreased, the pro-autophagic ULK1 gene increased and the chaperone-mediated autophagy regulator PHLPP1 decreased in OB GDM(–) vs. NW. On the other hand, PHLPP1 expression increased in OB GDM(+) vs. OB GDM(–). When analyzing results in relation to fetal sex, we found sexual dimorphism for both antioxidant and CMA-related gene expressions. These preliminary results can pave the way for further analyses aimed at elucidating the placental autophagy role in metabolic pregnancy disorders and its potential targetability for the treatment of diabetes outcomes.
Mediterranean Diet and Type 2 Diabetes Mellitus: A Perpetual Inspiration for the Scientific World. A Review
Authors: Milenkovic Tatjana , Bozhinovska Nadica , Macut Djuro , Bjekic-Macut Jelica , Rahelic Dario , Velija Asimi Zelija , Burekovic Azra
Published: April 2021

For the past 80 years, the effect of the Mediterranean diet on overall health has been a constant topic of interest among medical and scientific researchers. Parallel with the persistent global rise of cases of type 2 diabetes, many studies conducted in the past 20 years have shown the benefits of the Mediterranean lifestyle for people with, or at risk of developing, type 2 diabetes mellitus. However, despite the large body of evidence, concerns exist amongst scientists regarding the reliability of the data related to this topic. This review offers a glimpse of the onset of the Mediterranean diet and follows its significant impact on the prevention and treatment of type 2 diabetes. There is a constant rise in type 2 diabetes cases on the Balkan Peninsula and North Macedonia in particular. Having in mind that North Macedonia, as well as most of the countries on the Balkans have low to middle income, there is a need for a certain affordable dietary pattern to ameliorate the rise in diabetes incidence, as well as improve the glycemic control. We did a review based on the available literature regarding Mediterranean diet and people with or at risk of developing type 2 diabetes mellitus, its effects on glycemic control, lipid profile and metabolic outcome.
High-Fat Foods and FODMAPs Containing Gluten Foods Primarily Contribute to Symptoms of Irritable Bowel Syndrome in Korean Adults
Authors: Na Woori , Lee Yeji , Kim Hyeji , Kim Yong Sung , Sohn Cheongmin
Published: April 2021

Dietary control plays an important role in the treatment of irritable bowel syndrome (IBS). However, few studies have examined the relationship between dietary intake and symptoms of IBS in Koreans. The current cross-sectional study aimed to examine the diet in food consumption and nutrient intake in Korean adults aged 20 to 40 with IBS. The data collected were completed by 857 subjects using a community-based web survey. The questionnaire covered functional bowel disorders based on Rome III, the semi-quantitative Food Frequency Questionnaire (SQ-FFQ), and the food items causing symptoms. In total, 186 of 857 subjects (21.7%) were diagnosed with IBS. The non-IBS group had a fat intake of 76.9 ± 47.9 g/day, while the IBS group had a fat intake of 86.6 ± 55.1 g/day (p = 0.014). The non-IBS group had a total fermentable oligosaccharide, disaccharide, monosaccharide, and polyol (FODMAP) intake of 12.6 ± 9.7 g/day, whereas the IBS group had a total FODMAP intake of 13.9 ± 9.9 g/day (p = 0.030). Foods that contributed to the onset of symptoms in the IBS group were instant noodles (70.8%), Chinese noodles with vegetables and seafood (68.7%), pizza (67.2%), and black bean sauce noodles (66.3%) which are mostly classified as high fat and high gluten foods. The dietary intake of IBS patients differs from that of non-IBS subjects. Increased intake of gluten-containing or high-fat foods due to the westernized diet caused more IBS symptoms than high FODMAPs and dairy products in Korean adults in their 20 s to 40 s.
Effects of a Prenatal Lifestyle Intervention in Routine Care on Maternal Health Behaviour in the First Year Postpartum—Secondary Findings of the Cluster-Randomised GeliS Trial
Authors: Geyer Kristina , Spies Monika , Günther Julia , Hoffmann Julia , Raab Roxana , Meyer Dorothy , Rauh Kathrin , Hauner Hans
Published: April 2021

Lifestyle interventions during pregnancy were shown to beneficially influence maternal dietary behaviour and physical activity, but their effect on health behaviour after delivery is unclear. The objective of this secondary analysis was to investigate the sustained effect of a lifestyle intervention in routine care on maternal health behaviour during the first year postpartum. The cluster-randomised controlled “Healthy living in pregnancy” (GeliS) study included 2286 pregnant women. Data on maternal health behaviour were collected at 6–8 weeks (T1pp) and one year postpartum (T2pp) using validated questionnaires. The intervention group showed a lower mean intake of fast food (T1pp: p = 0.016; T2pp: p < 0.001) and soft drinks (T1pp: p < 0.001), a higher mean intake of vegetables (T2pp: p = 0.015) and was more likely to use healthy oils for meal preparation than the control group. Dietary quality rated by a healthy eating index was higher in the intervention group (T1pp: p = 0.093; T2pp: p = 0.043). There were minor trends towards an intervention effect on physical activity behaviour. The proportion of smokers was lower in the intervention group (p < 0.001, both time points). The lifestyle intervention within routine care modestly improved maternal postpartum dietary and smoking behaviours.
In Vitro Drug Release, Permeability, and Structural Test of Ciprofloxacin-Loaded Nanofibers
Authors: Uhljar Luca Éva , Kan Sheng Yuan , Radacsi Norbert , Koutsos Vasileios , Szabó-Révész Piroska , Ambrus Rita
Published: April 2021

Nanofibers of the poorly water-soluble antibiotic ciprofloxacin (CIP) were fabricated in the form of an amorphous solid dispersion by using poly(vinyl pyrrolidone) as a polymer matrix, by the low-cost electrospinning method. The solubility of the nanofibers as well as their in vitro diffusion were remarkably higher than those of the CIP powder or the physical mixture of the two components. The fiber size and morphology were optimized, and it was found that the addition of the CIP to the electrospinning solution decreased the nanofiber diameter, leading to an increased specific surface area. Structural characterization confirmed the interactions between the drug and the polymer and the amorphous state of CIP inside the nanofibers. Since the solubility of CIP is pH-dependent, the in vitro solubility and dissolution studies were executed at different pH levels. The nanofiber sample with the finest morphology demonstrated a significant increase in solubility both in water and pH 7.4 buffer. Single medium and two-stage biorelevant dissolution studies were performed, and the release mechanism was described by mathematical models. Besides, in vitro diffusion from pH 6.8 to pH 7.4 notably increased when compared with the pure drug and physical mixture. Ciprofloxacin-loaded poly(vinyl pyrrolidone) (PVP) nanofibers can be considered as fast-dissolving formulations with improved physicochemical properties.
Amorphous Solid Dispersion Tablets Overcome Acalabrutinib pH Effect in Dogs
Authors: Mudie Deanna M. , Stewart Aaron M. , Rosales Jesus A. , Biswas Nishant , Adam Molly S. , Smith Adam , Craig Christopher D. , Morgen Michael M. , Vodak David T.
Published: April 2021

Calquence® (crystalline acalabrutinib), a commercially marketed tyrosine kinase inhibitor (TKI), exhibits significantly reduced oral exposure when taken with acid-reducing agents (ARAs) due to the low solubility of the weakly basic drug at elevated gastric pH. These drug–drug interactions (DDIs) negatively impact patient treatment and quality of life due to the strict dosing regimens required. In this study, reduced plasma drug exposure at high gastric pH was overcome using a spray-dried amorphous solid dispersion (ASD) comprising 50% acalabrutinib and 50% hydroxypropyl methylcellulose acetate succinate (HPMCAS, H grade) formulated as an immediate-release (IR) tablet. ASD tablets achieved similar area under the plasma drug concentration–time curve (AUC) at low and high gastric pH and outperformed Calquence capsules 2.4-fold at high gastric pH in beagle dogs. In vitro multicompartment dissolution testing conducted a priori to the in vivo study successfully predicted the improved formulation performance. In addition, ASD tablets were 60% smaller than Calquence capsules and demonstrated good laboratory-scale manufacturability, physical stability, and chemical stability. ASD dosage forms are attractive for improving patient compliance and the efficacy of acalabrutinib and other weakly basic drugs that have pH-dependent absorption.
Novel Magnetic Inorganic Composites: Synthesis and Characterization
Authors: Natali Marco , Tamburini Sergio , Bertani Roberta , Desideri Daniele , Mozzon Mirto , Pavarin Daniele , Spizzo Federico , Del Bianco Lucia , Zorzi Federico , Sgarbossa Paolo
Published: April 2021

The addition of magnetic particles to inorganic matrices can produce new composites exhibiting intriguing properties for practical applications. It has been previously reported that the addition of magnetite to concrete improves its mechanical properties and durability in terms of water and chloride ions absorption. Here we describe the preparation of novel magnetic geopolymers based on two different matrices (G1 without inert aggregates and G2 with inert quartz aggregates) containing commercial SrFe12O19 particles with two weight concentrations, 6% and 11%. The composites’ characterization, including chemical, structural, morphological, and mechanical determinations together with magnetic and electrical measurements, was carried out. The magnetic study revealed that, on average, the SrFe12O19 magnetic particles can be relatively well dispersed in the inorganic matrix. A substantial increase in the composite samples’ remanent magnetization was obtained by embedding in the geopolymer SrFe12O19 anisotropic particles at a high concentration under the action of an external magnetic field during the solidification process. The new composites exhibit good mechanical properties (as compressive strength), higher than those reported for high weight concretes bearing a similar content of magnetite. The impedance measurements indicate that the electrical resistance is mainly controlled by the matrix’s chemical composition and can be used to evaluate the geopolymerization degree.
Polymer-Based Smart Drug Delivery Systems for Skin Application and Demonstration of Stimuli-Responsiveness
Authors: Van Gheluwe Louise , Chourpa Igor , Gaigne Coline , Munnier Emilie
Published: April 2021

Progress in recent years in the field of stimuli-responsive polymers, whose properties change depending on the intensity of a signal, permitted an increase in smart drug delivery systems (SDDS). SDDS have attracted the attention of the scientific community because they can help meet two current challenges of the pharmaceutical industry: targeted drug delivery and personalized medicine. Controlled release of the active ingredient can be achieved through various stimuli, among which are temperature, pH, redox potential or even enzymes. SDDS, hitherto explored mainly in oncology, are now developed in the fields of dermatology and cosmetics. They are mostly hydrogels or nanosystems, and the most-used stimuli are pH and temperature. This review offers an overview of polymer-based SDDS developed to trigger the release of active ingredients intended to treat skin conditions or pathologies. The methods used to attest to stimuli-responsiveness in vitro, ex vivo and in vivo are discussed.
Influence of Ultraviolet Radiation on Mechanical Properties of a Photoinitiator Compounded High Vinyl Styrene–Butadiene–Styrene Block Copolymer
Authors: Datta Sanjoy , Stocek Radek , Naskar Kinsuk
Published: April 2021

Ultraviolet curing of elastomers is a special curing technique that has gained importance over the conventional chemical crosslinking method, because the former process is faster, and thus, time-saving. Usually, a suitable photoinitiator is required to initiate the process. Ultraviolet radiation of required frequency and intensity excites the photoinitiator which abstracts labile hydrogen atoms from the polymer with the generation of free radicals. These radicals result in crosslinking of elastomers via radical–radical coupling. In the process, some photodegradation may also take place. In the present work, a high vinyl (~50%) styrene–butadiene–styrene (SBS) block copolymer which is a thermoplastic elastomer was used as the base polymer. An attempt was made to see the effect of ultraviolet radiation on the mechanical properties of the block copolymer. The process variables were time of exposure and photoinitiator concentration. Mechanical properties like tensile strength, elongation at break, modulus at different elongations and hardness of the irradiated samples were studied and compared with those of unirradiated ones. In this S-B-S block copolymer, a relatively low exposure time and low photoinitiator concentration were effective in obtaining optimized mechanical properties. Infrared spectroscopy, contact angle and scanning electron microscopy were used to characterize the results obtained from mechanical measurements.
Demineralized Dentin Matrix Particle-Based Bio-Ink for Patient-Specific Shaped 3D Dental Tissue Regeneration
Authors: Han Jonghyeuk , Jeong Wonwoo , Kim Min-Kyeong , Nam Sang-Hyeon , Park Eui-Kyun , Kang Hyun-Wook
Published: April 2021

Demineralized dentin matrix (DDM)-based materials have been actively developed and are well-known for their excellent performance in dental tissue regeneration. However, DDM-based bio-ink suitable for fabrication of engineered dental tissues that are patient-specific in terms of shape and size, has not yet been developed. In this study, we developed a DDM particle-based bio-ink (DDMp bio-ink) with enhanced three-dimensional (3D) printability. The bio-ink was prepared by mixing DDM particles and a fibrinogen–gelatin mixture homogeneously. The effects of DDMp concentration on the 3D printability of the bio-ink and dental cell compatibility were investigated. As the DDMp concentration increased, the viscosity and shear thinning behavior of the bio-ink improved gradually, which led to the improvement of the ink’s 3D printability. The higher the DDMp content, the better were the printing resolution and stacking ability of the 3D printing. The printable minimum line width of 10% w/v DDMp bio-ink was approximately 252 μm, whereas the fibrinogen–gelatin mixture was approximately 363 μm. The ink’s cytocompatibility test with dental pulp stem cells (DPSCs) exhibited greater than 95% cell viability. In addition, as the DDMp concentration increased, odontogenic differentiation of DPSCs was significantly enhanced. Finally, we demonstrated that cellular constructs with 3D patient-specific shapes and clinically relevant sizes could be fabricated through co-printing of polycaprolactone and DPSC-laden DDMp bio-ink.
Fire Behavior of Thermally Thin Materials in Cone Calorimeter
Authors: El Gazi Marouane , Sonnier Rodolphe , Giraud Stéphane , Batistella Marcos , Basak Shantanu , Dumazert Loïc , Hajj Raymond , El Hage Roland
Published: April 2021

In this study, a representative set of thermally thin materials including various lignocellulosic and synthetic fabrics, dense wood, and polypropylene sheets were tested using a cone calorimeter at different heat fluxes. Time-to-ignition, critical heat flux, and peak of heat release rate (pHRR) were the main parameters considered. It appears that the flammability is firstly monitored by the sample weight. Especially, while the burning rate of thermally-thin materials does never reach a steady state in cone calorimeter, their pHRR appears to be mainly driven by the fire load (i.e., the product of sample weight and effective heat of combustion) with no or negligible influence of textile structure. A simple phenomenological model was proposed to calculate the pHRR taking into account only three parameters, namely heat flux, sample weight, and effective heat of combustion. The model allows predicting easily the peak of heat release rate, which is often considered as the main single property informing about the fire hazard. It also allows drawing some conclusions about the flame retardant strategies to reduce the pHRR..