bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the Behavior Characteristics of Pedestrian Crowd Weaving Flow in Transport Terminal
Authors: Yao Liya , Sun Lishan , Zhang Zhiyong , Wang Shuwei , Rong Jian
Published: September 2012

Due to the poor transfer organization in urban public transport terminal, pedestrian crowd are often forced to weaving in their transfer flow lines. Frequent weaving behaviors not only decrease passengers’ transfer comfort, but may also trigger serious crowd disaster such as trampling. In order to get accurate understanding of the weaving features of pedestrian crowd and analyze the relevant evolution law, researches have been conducted on the basis of field investigation. First, the typical weaving phenomenon were defined and classified, and a microscopic parameters system of pedestrian crowd weaving flow was constructed. The detection and quantification methods of multiple indicator parameters were also given. Then, correlation between different behavioral parameters was analyzed based on the survey data of weaving pedestrian crowd on the stairs of DongZhiMen (DZM) hub. The basic characteristics and evolution law of the weaving behaviors were then discussed, and conclusions were drawn.
Organizational Learning in Schools under Sanction
Authors: Finnigan Kara S. , Daly Alan J. , Stewart Tricia J.
Published: September 2012

The focus on “school turnaround” has become central to policy and practice in the United States as a result of school accountability, yet little remains known about school improvement under sanction. This study uses theories of organizational learning to understand the processes through which educators search for and adopt reform strategies, as well as the extent to which these schools’ organizational culture and climate are conducive to this type of learning. Our mixed methods study involves document analysis, intensive case studies, and a survey of teachers in schools under sanction in a large urban school district in the USA. We found limited evidence of organizational learning, and instead evidence suggested superficial use of restructuring planning, rare diagnoses of root causes of low performance, and limited engagement in learning processes of school staff. In addition, schools relied on exploitation resulting in the recycling of previous practices. In part, the limited organizational learning in evidence was the result of structures and climates within these low-performing schools that inhibited a more learning-oriented approach to reform. Our study has implications for school improvement under accountability policies as it uncovers important challenges that limit organizational learning and, as a result, school improvement under sanction.
Assessing Nutrient Intake and Nutrient Status of HIV Seropositive Patients Attending Clinic at Chulaimbo Sub-District Hospital, Kenya
Authors: Onyango Agatha Christine , Walingo Mary Khakoni , Mbagaya Grace , Kakai Rose
Published: September 2012

Background. Nutritional status is an important determinant of HIV outcomes.Objective. To assess the nutrient intake and nutrient status of HIV seropositive patients attending an AIDS outpatient clinic, to improve the nutritional management of HIV-infected patients.Design. Prospective cohort study.Setting. Comprehensive care clinic in Chulaimbo Sub-District Hospital, Kenya.Subjects. 497 HIV sero-positive adults attending the clinic.Main Outcome Measures. Evaluation of nutrient intake using 24-hour recall, food frequency checklist, and nutrient status using biochemical assessment indicators (haemoglobin, creatinine, serum glutamate pyruvate (SGPT) and mean corpuscular volume (MCV)).Results. Among the 497 patients recruited (M : F sex ratio: 1.4, mean age: 39 years ± 10.5 y), Generally there was inadequate nutrient intake reported among the HIV patients, except iron (10.49 ± 3.49 mg). All the biochemical assessment indicators were within normal range except for haemoglobin 11.2 g/dL (11.4 ± 2.60 male and 11.2 ± 4.25 female).Conclusions. Given its high frequency, malnutrition should be prevented, detected, monitored, and treated from the early stages of HIV infection among patients attending AIDS clinics in order to improve survival and quality of life.
DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation
Authors: Patel Nikul , Black Jennifer , Chen Xi , Marcondes A. Mario , Grady William M. , Lawlor Elizabeth R. , Borinstein Scott C.
Published: September 2012

The role of aberrant DNA methylation in Ewing sarcoma is not completely understood. The methylation status of 503 genes in 52 formalin-fixed paraffin-embedded EWS tumors and 3 EWS cell lines was compared to human mesenchymal stem cell primary cultures (hMSCs) using bead chip methylation analysis. Relative expression of methylated genes was assessed in 5-Aza-2-deoxycytidine-(5-AZA)-treated EWS cell lines and in a cohort of primary EWS samples and hMSCs by gene expression and quantitative RT-PCR. 129 genes demonstrated statistically significant hypermethylation in EWS tumors compared to hMSCs. Thirty-six genes were profoundly methylated in EWS and unmethylated in hMSCs. 5-AZA treatment of EWS cell lines resulted in upregulation of expression of hundreds of genes including 162 that were increased by at least 2-fold. The expression of 19 of 36 candidate hypermethylated genes was increased following 5-AZA. Analysis of gene expression from an independent cohort of tumors confirmed decreased expression of six of nineteen hypermethylated genes (AXL, COL1A1, CYP1B1, LYN, SERPINE1,) andVCAN. Comparing gene expression and DNA methylation analyses proved to be an effective way to identify genes epigenetically regulated in EWS. Further investigation is ongoing to elucidate the role of these epigenetic alterations in EWS pathogenesis.
Development of Personal Wellness Information Model for Pervasive Healthcare
Authors: Seppälä Antto , Nykänen Pirkko , Ruotsalainen Pekka
Published: September 2012

Pervasive healthcare and citizen-centered care paradigm are moving the healthcare outside the hospital environment. Healthcare delivery is becoming more personalized and decentralized, focusing on prevention and proactive services with a complete view of health and wellbeing. The concept of wellness has been used to describe this holistic view of health, which focuses on physical, social, and mental well-being. Pervasive computing makes it possible to collect information and offer services anytime and anywhere. To support pervasive healthcare with wellness approaches, semantic interoperability is needed between all actors and information sources in the ecosystem. This study focuses on the domain of personal wellness and analyzes related concepts, relationships, and environments. As a result of this study, we have created an information model that focuses on the citizens’ perspectives and conceptualizations of personal wellness. The model has been created based on empirical research conducted with focus groups.
Bilateral Asynchronous Renal Cell Carcinoma with Metastatic Involvement of the Tongue
Authors: Ghazali Naseem , Davis Charlotte , Barrett A. W. , Tighe John V.
Published: September 2012

Renal cell carcinoma (RCC) has a propensity for distant organ metastasis and late recurrence, involving not only the ipsilateral but also contralateral kidney. Lingual metastasis by RCC is rare. We present an unusual case of bilateral asynchronous RCC. Involvement of the right kidney was discovered only after a metastatic tongue lesion was diagnosed. The original RCC had been treated by left nephrectomy 14 years previously. Due to end-stage primary pulmonary malignancy, and poor function of the remaining kidney, immunotherapy was unsuitable. Palliative local resection of the lingual metastasis alleviated functional difficulties and was preventative against airway obstruction, but the patient died five months later.
Application of the Poor Man′s Navier‐Stokes Equations to Real‐Time Control of Fluid Flow
Authors: Polly James B. , McDonough J. M.
Published: September 2012

Control of fluid flow is an important, underutilized process possessing potential benefits ranging from avoidance of separation and stall on aircraft wings to reduction of friction in oil and gas pipelines to mitigation of noise from wind turbines. But the Navier‐Stokes (N.‐S.) equations, whose solutions describe such flows, consist of a system of time‐dependent, multidimensional, nonlinear partial differential equations (PDEs) which cannot be solved in real time using current computing hardware. The poor man′s Navier‐Stokes (PMNS) equations comprise a discrete dynamical system that is algebraic—hence, easily (and rapidly) solved—and yet which retains many (possibly all) of the temporal behaviors of the PDE N.‐S. system at specific spatial locations. Herein, we outline derivation of these equations and discuss their basic properties. We consider application of these equations to the control problem by adding a control force. We examine the range of behaviors that can be achieved by changing this control force and, in particular, consider controllability of this (nonlinear) system via numerical experiments. Moreover, we observe that the derivation leading to the PMNS equations is very general and may be applied to a wide variety of problems governed by PDEs and (possibly) time‐delay ordinary differential equations such as, for example, models of machining processes.
Immunological Evaluation and Comparison of Different EV71 Vaccine Candidates
Authors: Chou Ai-Hsiang , Liu Chia-Chyi , Chang Jui-Yuan , Lien Shu-Pei , Guo Meng-Shin , Tasi Hau-Pong , Hsiao Kuang-Nan , Liu Shih-Jen , Sia Charles , Wu Suh-Chin , Lee Min-Shi , Hsiao Chia-Hsin , Wang Jen-Ren , Chow Yen-Hung , Chong Pele
Published: September 2012

Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are major causative agents of hand, foot, and mouth diseases (HFMDs), and EV71 is now recognized as an emerging neurotropic virus in Asia. Effective medications and/or prophylactic vaccines against HFMD are not available. The current results from mouse immunogenicity studies using in-house standardized RD cell virus neutralization assays indicate that (1) VP1 peptide (residues 211–225) formulated with Freund’s adjuvant (CFA/IFA) elicited low virus neutralizing antibody response (1/32 titer); (2) recombinant virus-like particles produced from baculovirus formulated with CFA/IFA could elicit good virus neutralization titer (1/160); (3) individual recombinant EV71 antigens (VP1, VP2, and VP3) formulated with CFA/IFA, only VP1 elicited antibody response with 1/128 virus neutralization titer; and (4) the formalin-inactivated EV71 formulated in alum elicited antibodies that cross-neutralized different EV71 genotypes (1/640), but failed to neutralize CVA16. In contrast, rabbits antisera could cross-neutralize strongly against different genotypes of EV71 but weakly against CVA16, with average titers 1/6400 and 1/32, respectively. The VP1 amino acid sequence dissimilarity between CVA16 and EV71 could partially explain why mouse antibodies failed to cross-neutralize CVA16. Therefore, the best formulation for producing cost-effective HFMD vaccine is a combination of formalin-inactivated EV71 and CAV16 virions.
Mitochondrial Mutations are Associated with Atherosclerotic Lesions in the Human Aorta
Authors: Sobenin Igor A. , Sazonova Margarita A. , Postnov Anton Y. , Bobryshev Yuri V. , Orekhov Alexander N.
Published: September 2012

Somatic mutations of the human mitochondrial genome can be a possible determinant of atherosclerosis. To test this possibility, forty mitochondrial mutations were analyzed in the present study in order to see which of these mutations might be associated with atherosclerosis. Ten mitochondrial mutations belonging to mitochondrial genes MT-RNR1 (rRNA 12S); MT-TL1 (tRNA-Leu, recognizes UUR); MT-TL2 (tRNA-Leu, recognizes CUN); MT-ND1, MT-ND2, MT-ND5, and MT-ND6 (subunits 1, 2, 5, and 6, respectively, of NADH dehydrogenase); and MT-CYB (cytochrome b) were potentially associated with atherosclerosis. From 29% (2 of 7 aortic samples) upto 86% (6 of 7 aortic samples) of aortic samples had a significant difference between atherosclerotic plaques and unaffected tissue, with the respect to the level of heteroplasmy for each mutation. Further, the homogenates of affected and normal intimae of 22 aortas were compared to reveal the average level of heteroplasmy for the above-mentioned 10 mutations. For five mutations, the mean level of heteroplasmy was significantly different in atherosclerotic intimal homogenates in comparison with the unaffected tissue. These mutations were A1555G, C3256T, T3336C, G13513A, and G15059A. Thus, it was demonstrated that at least five mitochondrial mutations occurring in MT-RNR1, MT-TL1, MT-ND2, MT-ND5, and MT-CYB genes are associated with atherosclerosis.
Convergence Analysis of the Preconditioned Group Splitting Methods in Boundary Value Problems
Authors: Mohd Ali Norhashidah Hj. , Mohammed Saeed Abdulkafi
Published: September 2012

The construction of a specific splitting‐type preconditioner in block formulation applied to a class of group relaxation iterative methods derived from the centred and rotated (skewed) finite difference approximations has been shown to improve the convergence rates of these methods. In this paper, we present some theoretical convergence analysis on this preconditioner specifically applied to the linear systems resulted from these group iterative schemes in solving an elliptic boundary value problem. We will theoretically show the relationship between the spectral radiuses of the iteration matrices of the preconditioned methods which affects the rate of convergence of these methods. We will also show that the spectral radius of the preconditioned matrices is smaller than that of their unpreconditioned counterparts if the relaxation parameter is in a certain optimum range. Numerical experiments will also be presented to confirm the agreement between the theoretical and the experimental results.
The Effect of Ultrasound Stimulation on the Cytoskeletal Organization of Chondrocytes Seeded in Three-Dimensional Matrices
Authors: Noriega Sandra , Hasanova Gulnara , Subramanian Anuradha
Published: September 2012

The impact of low-intensity diffuse ultrasound (LIDUS) stimulation on the cytoskeletal organization of chondrocytes seeded in three-dimensional (3D) scaffolds was evaluated. Chondrocytes seeded on 3D chitosan matrices were exposed to LIDUS at 5.0 MHz (approx. 15 kPa, 51 s, 4 applications/day) in order to study the organization of actin, tubulin and vimentin. The results showed that actin presented a punctate cytosolic distribution and tubulin presented a quasiparallel organization of microtubules, whereas vimentin distribution was unaffected. Chondrocytes seeded on 3D scaffolds responded to US stimulation by the disruption of actin stress fibers and were sensitive to the presence of Rho-activated kinase (ROCK) inhibitor (Y27632). The gene expression of ROCK-I, a key element in the formation of stress fibers and mDia1, was significantly upregulated under the application of US. We conclude that the results of both the cytoskeletal analyses and gene expression support the argument that the presence of punctate actin upon US stimulation was accompanied by the upregulation of the RhoA/ROCK pathway.
Primordial Germ Cells (Spermatogonial Stem Cells) of Bullfrogs Express Sex Hormone-Binding Globulin and Steroid Receptors during Seasonal Spermatogenesis
Authors: Caneguim Breno Henrique , Beltrame Flávia Luciana , da Luz Juliana Silva , Valentini Sandro Roberto , Cerri Paulo Sérgio , Sasso-Cerri Estela
Published: September 2012

In vertebrate species, testosterone seems to inhibit spermatogonial differentiation and proliferation. However, this androgen can also be converted, via aromatase, into estrogen which stimulates spermatogonial differentiation and mitotic activity. During seasonal spermatogenesis of adult bullfrogs Lithobates catesbeianus, primordial germ cells (PGCs) show enhanced testosterone cytoplasm immunoexpression in winter; however, in summer, weak or no testosterone immunolabelling was observed. The aim of this study was to confirm if PGCs express stem cell markers – alkaline phosphatase (AP) activity and GFRα1 (glial-cell-line-derived neurotrophic factor) – and verify whether testosterone is maintained in these cells by androgen receptors (ARs) and/or sex hormone-binding globulin (SHBG) in winter. Furthermore, regarding the possibility that testosterone is converted into estrogen by PGCs in summer, the immunoexpression of estrogen receptor (ER)β was investigated. Bullfrog testes were collected in winter and in summer and were embedded in glycol methacrylate for morphological analyses or in paraffin for the histochemical detection of AP activity. GFRα1, AR, SHBG and ERβ expression were detected by Western blot and immunohistochemical analyses. The expression of AP activity and GFRα1 in the PGCs suggest that these cells are spermatogonial stem cells. In winter, the cytoplasmic immunoexpression of ARs and SHBG in the PGCs indicates that testosterone is maintained by these proteins in these cells. The cytoplasmic immunoexpression of ERβ, in summer, also points to an ER-mediated action of estrogen in PGCs. The results indicate a participation of testosterone and estrogen in the control of the primordial spermatogonia during the seasonal spermatogenesis of L. catesbeianus.
Reduced Number of Circulating Endothelial Progenitor Cells (CD133+/KDR+) in Patients with Plaque Psoriasis
Authors: Batycka-Baran Aleksandra , Paprocka Maria , Krawczenko Agnieszka , Kantor Aneta , Duś Danuta , Szepietowski Jacek C.
Published: September 2012

Background: Psoriasis is associated with an increased cardiovascular risk. Circulating endothelial progenitor cells (CEPCs) play a significant role in the maintenance of vascular homeostasis. Objective: The aim of this study was to evaluate the number of CEPCs in patients with psoriasis compared to controls and assess possible correlations between the number of these cells and the plasma levels of vascular endothelial growth factor (VEGF), soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) and clinical features of psoriasis. Methods: The number of CEPCs, identified as CD133+/KDR+ cells, was determined with flow cytometry in peripheral blood of psoriatic patients (n = 63) and controls (n = 31). The plasma levels of VEGF and sVEGFR-1 were measured with enzyme-linked immunosorbent assay. Results: The number of CEPCs was significantly reduced in psoriatic patients compared with controls (p = 0.000026) and inversely correlated with disease severity (R = –0.283; p = 0.0248). Conclusion: A reduced number of CEPCs may contribute to endothelial dysfunction in patients with psoriasis.
Impact of Estrogen Replacement throughout Childhood on Growth, Pituitary-Gonadal Axis and Bone in a 46,XX Patient with CYP19A1 Deficiency
Authors: Janner Marco , Flück Christa E. , Mullis Primus E.
Published: September 2012

Background: The adequate replacement dose of estrogens during infancy and childhood is still not known in girls. Aromatase deficiency offers an excellent model to study how much estrogens are needed during infancy, childhood and adulthood. Objectives: We studied the impact of oral 17β-estradiol treatment, on longitudinal growth, bone age maturation, pituitary gonadotropin feedback, multicystic ovaries and bone mass in the long-term follow-up of a girl compound heterozygote for two point mutations of the CYP19A1 gene. Results: Low doses of 17β-estradiol were needed to achieve normal height velocity and adequate bone age maturation from early childhood on. Serum estradiol levels needed for breast development and for the appearance of an endometrial reflex were not sufficient to achieve physiological gonadotropin levels. Without 17β-estradiol treatment the ovaries of the patient showed a multicystic appearance, which reversed on 17β-estradiol replacement. Bone mass was within normal ranges during the whole follow-up period. Conclusion: In summary, we have shown that estradiol is needed not only in puberty but also in childhood for normal growth, bone maturation and achievement of normal bone mass. Particularly, this observation underscores the importance of early low-dose estrogen replacement also in other estrogen-deficient conditions as for instance in Turner’s syndrome.
Combined Screening for Preeclampsia and Small for Gestational Age at 11–13 Weeks
Authors: Poon Leona C.Y. , Syngelaki Argyro , Akolekar Ranjit , Lai Jonathan , Nicolaides Kypros H.
Published: September 2012

Objective: To combine a specific algorithm for small for gestational age (SGA) without preeclampsia (PE) and another algorithm for PE in the prediction of SGA and PE. Methods: This was a screening study of singleton pregnancies at 11–13 weeks including 1,426 (2.3%) that subsequently developed PE, 3,168 (5.1%) that delivered SGA neonates and 57,458 that were unaffected by PE and SGA. We developed a prediction algorithm for SGA requiring delivery before 37 weeks’ gestation (preterm-SGA) from maternal characteristics, uterine artery pulsatility index, mean arterial pressure, serum pregnancy-associated plasma protein-A and placental growth factor multiple of the median values. We then examined the performance of this algorithm individually and in combination with a previously reported algorithm for early-PE in the prediction of SGA and PE. Results: When screen positivity was defined by risk cutoff of 1:200 using the algorithm for early-PE and the risk cutoff of 1:150 using the algorithm for preterm-SGA, the false positive rate was 10.9% and the detection rates of early-PE, late-PE, preterm-SGA and term-SGA were 95.3, 45.6, 55.5 and 44.3%, respectively. Conclusions: Effective first-trimester screening for early-PE and preterm-SGA can be provided by the combined use of the specific algorithms.
Measuring Academic Performance for Healthcare Researchers with the H Index: Which Search Tool Should Be Used?
Authors: Patel Vanash M. , Ashrafian Hutan , Almoudaris Alex , Makanjuola Jonathan , Bucciarelli-Ducci Chiara , Darzi Ara , Athanasiou Thanos
Published: September 2012

Objectives: To compare H index scores for healthcare researchers returned by Google Scholar, Web of Science and Scopus databases, and to assess whether a researcher’s age, country of institutional affiliation and physician status influences calculations. Subjects and Methods: One hundred and ninety-five Nobel laureates in Physiology and Medicine from 1901 to 2009 were considered. Year of first and last publications, total publications and citation counts, and the H index for each laureate were calculated from each database. Cronbach’s alpha statistics was used to measure the reliability of H index scores between the databases. Laureate characteristic influence on the H index was analysed using linear regression. Results: There was no concordance between the databases when considering the number of publications and citations count per laureate. The H index was the most reliably calculated bibliometric across the three databases (Cronbach’s alpha = 0.900). All databases returned significantly higher H index scores for younger laureates (p < 0.0001). Google Scholar and Web of Science returned significantly higher H index for physician laureates (p = 0.025 and p = 0.029, respectively). Country of institutional affiliation did not influence the H index in any database. Conclusion: The H index appeared to be the most consistently calculated bibliometric between the databases for Nobel laureates in Physiology and Medicine. Researcher-specific characteristics constituted an important component of objective research assessment. The findings of this study call to question the choice of current and future academic performance databases.
Public Health Action in Genomics Is Now Needed beyond Newborn Screening
Authors: Bowen M.S. , Kolor K. , Dotson W.D. , Ned R.M. , Khoury M.J.
Published: September 2012

For decades, newborn screening was the only public health program in the US focused on reducing morbidity, mortality and disability in people affected by genetic conditions. The landscape has changed, however, as evidence-based recommendations are now available for several other genomic applications that can save lives now in the US. Many more such applications are expected to emerge in the next decade. An action plan, based on evidence, provides the impetus for a new paradigm for public health practice in genomics across the lifespan using established multilevel processes as a guide. These include policy interventions, education, clinical interventions, and surveillance. Applying what we know today in hereditary breast/ovarian cancer, Lynch syndrome and familial hypercholesterolemia has the potential to affect thousands of people in the US population every year. Enhanced partnerships between genetic and nongenetic providers of clinical medicine and public health are needed to overcome the challenges for implementing genomic medicine applications both now and in the future.
Extravasation of Intravesical Chemotherapy for Non-Muscle-Invasive Bladder Cancer
Authors: Mertens Laura S. , Meinhardt Wim , Rier Walther B. , Nooter Ronald I. , Horenblas Simon
Published: September 2012

Purpose: To report our experience with symptomatic extravasation of intravesical chemotherapy administered within 24 h after transurethral resection (TUR) over the past 10 years. Methods: We identified all consecutive patients who presented with symptomatic extravasation of intravesical chemotherapy following TUR between 2001 and 2011. We assessed the severity of the postoperative complications using the modified Clavien system. Results: We identified 9 patients (mean age 59, range 40–76 years) with symptomatic extravasation. One patient had grade II, 2 had grade IIIa, and 5 patients had grade IIIb complications according to the Clavien system. Surgery was needed in 6 of 9 patients. One required ICU management (Clavien IV). No patients died in the postoperative course. Conclusion: Extravasation can cause severe complications and diagnosis is often protracted. Considering the growing practice of immediate intravesical instillations, the number of patients with symptomatic extravasation is expected to rise. An increased awareness of this possible complication is warranted.
A Rare Case of Isolated Kidney Cysticercosis
Authors: Potic M. , Ignjatovic I. , Velickovic Jankovic L.
Published: September 2012

A rare case of isolated kidney cysticercosis is presented. Clinical signs and diagnostic findings are unspecific. They are discussed and a conservative therapeutic approach is suggested.
Geometrical Stepper-Guided Navigation System for ProACT Implant under Transrectal Ultrasound Control: Preliminary Data
Authors: Crivellaro Simone , Tosco Lorenzo , Palazzetti Anna , Abbinante Maria , Martinez Guillermo , Kocjancic Ervin , Frea Bruno
Published: September 2012

Purpose: To describe a new geometrical stepper-guided navigation system for positioning ProACT®. Methods: The sizing of the stepper-guided navigation system was calculated using the distance from the ideal position of the device to anatomic referral points previously measured by ultrasound. The trocar and subsequently the device were maneuvered to the ideal position in accordance with the navigation system. Measurements: Treatment efficacy was evaluated with daily pad count, 1-hour pad test, Incontinence Quality of Life questionnaire (IQoL), visual analog scale and overall impression. Complications, balloon volume and number of adjustments were reported at 1, 3, 6 and 12 months follow-up visits. Results: Mean follow-up was 12 (range 3–19) months. Daily pad count showed 30 patients (71%) dry and 9 patients (21%) improved. 1 hour pad test showed 28 patients were dry (66%) and 11 patients improved (26%). IQoL increased from an average of 35.3 to 80. Average visual analog scale score was 8. Complications requiring device removal occurred in 3 patients (7%). Mean balloon volume was 3.1 ml. Conclusions: The stepper-guided navigation system to implant ProACT is feasible and extremely reproducible making this procedure more standardized.
Legal Liability and the Uncertain Nature of Risk Prediction: The Case of Breast Cancer Risk Prediction Models
Authors: Black L. , Knoppers B.M. , Avard D. , Simard J.
Published: September 2012

Background: The rapidity of technological change in genetics is not always matched by the uptake of this new knowledge into practice. Increasing genetic knowledge has already led to legal liability for those who have not used it properly, such as not informing patients or their families of potential genetic risk. A similar outcome is also of concern in the case of risk prediction models used for hereditary breast cancer. Results: No legal case has directly addressed the use of risk prediction models. However, as genetic medicine and risk prediction models become more widely used, the prospect of a lawsuit will also increase. Current case law is instructive on the circumstances under which medical liability actions could be pursued and circumstances under which liability is unlikely, such as the provision of faulty family history information by a patient. Conclusions: There is existing case law on family history and genetics that parallels in many respects the use of risk prediction models. However, the idea of a bad ‘prediction’ is a difficult legal concept. Outside of a plain misuse or failure to use a risk prediction model when circumstances clearly required it, there is little legal guidance presently available to determine the risk for medical liability.
Molecular Characterization of Xp Chromosome Deletion in a Fertile Cow
Authors: De Lorenzi L. , Rossi E. , Genualdo V. , Gimelli S. , Lasagna E. , Perucatti A. , Iannuzzi A. , Parma P.
Published: September 2012

A young cow of the Marchigiana breed (central Italy) with normal body conformation and external genitalia underwent routine cytogenetic analyses prior to its use for reproduction. After normal chromosome staining, only one X chromosome was observed with a normal diploid number (2n = 60) in all 200 studied cells. Subsequent cytogenetic analyses by using both CBA- and RBA-banding techniques evidenced that almost all the p arms of the other X chromosome was lacking. Detailed FISH-mapping analyses with BAC covering this Xp arm region demonstrated that this large chromosome region was deleted. RBA-banding showed that the deleted X was late replicating. CGH array analysis evidenced that deletion involves the Xp arm from the telomere to around 39.5 Mb, referring to the BosTau6 cattle genome assembly. This abnormality deletes about 40 Mb of the X chromosome sequence, but, despite the large number of genes deleted, none of them are programmed to escape from inactivation. This can explain the normal phenotype of the female which is actually pregnant. Finally, we evidenced, by analysis of an SNP mapped to the deleted region (SNP rs29024121), that the only normal (e.g. nondeleted) X chromosome present derives from the father. Hence, the deletion has a maternal origin.
Characterization of Maximal Respiratory Pressures in Healthy Children
Authors: Cox Desmond W. , Verheggen Maureen M. , Stick Stephen M. , Hall Graham L.
Published: September 2012

Background: Measurements of maximal voluntary inspiratory (Pimax) and expiratory (Pemax) pressures are used in the management of respiratory muscle disease. There is little data on the appropriate reference range, success rates, or repeatability of Pimax and Pemax in children or on methodological factors affecting test outcomes. Objectives: To determine Pimax and Pemax in healthy children and examine which published reference equations are best suited to a contemporary population. Secondary objectives were to assess within-test repeatability and the influence of lung volumes on Pimax and Pemax. Methods: Healthy children were prospectively recruited from the community on a volunteer basis and underwent spirometry, static lung volumes, and Pimax and Pemax testing. Results: Acceptable and repeatable (to within 20%) Pimax and Pemax were obtained in 156 children, with 105 (67%) children performing both Pimax and Pemax measurements to within 10% repeatability. The reference equations of Wilson et al. [Thorax 1984;39:535–538] best matched our healthy Caucasian children. There was an inverse relationship between Pimax and the percent of total lung capacity (TLC) at which the measurement was obtained (beta coefficient –0.96; 95% CI –1.52 to –0.39; p = 0.001), whereas at lung volumes of >80% TLC Pemax was independent of lung volume (p = 0.26). Conclusion: We demonstrated that the Wilson et al. [Thorax 1984;39:535–538] reference ranges are most suited for contemporary Caucasian Australasian children. However, robust multiethnic reference equations for maximal respiratory pressures are required. This study suggests that 10% within-test repeatability criteria are feasible in clinical practice, and that the use of lung volume measurements will improve the quality of maximal respiratory pressure measurements.
Statistical Computer Model Analysis of the Reciprocal and Recurrent Inhibitions of the Ia-EPSP in α-Motoneurons
Authors: Gradwohl G. , Grossman Y.
Published: September 2012

We simulate the inhibition of Ia-glutamatergic excitatory postsynaptic potential (EPSP) by preceding it with glycinergic recurrent (REN) and reciprocal (REC) inhibitory postsynaptic potentials (IPSPs). The inhibition is evaluated in the presence of voltage-dependent conductances of sodium, delayed rectifier potassium, and slow potassium in five [Formula: see text]-motoneurons (MNs). We distribute the channels along the neuronal dendrites using, alternatively, a density function of exponential rise (ER), exponential decay (ED), or a step function (ST). We examine the change in EPSP amplitude, the rate of rise (RR), and the time integral (TI) due to inhibition. The results yield six major conclusions. First, the EPSP peak and the kinetics depending on the time interval are either amplified or depressed by the REC and REN shunting inhibitions. Second, the mean EPSP peak, its TI, and RR inhibition of ST, ER, and ED distributions turn out to be similar for analogous ranges of G. Third, for identical G, the large variations in the parameters’ values can be attributed to the sodium conductance step ([Formula: see text]) and the active dendritic area. We find that small [Formula: see text] on a few dendrites maintains the EPSP peak, its TI, and RR inhibition similar to the passive state, but high [Formula: see text] on many dendrites decrease the inhibition and sometimes generates even an excitatory effect. Fourth, the MN's input resistance does not alter the efficacy of EPSP inhibition. Fifth, the REC and REN inhibitions slightly change the EPSP peak and its RR. However, EPSP TI is depressed by the REN inhibition more than the REC inhibition. Finally, only an inhibitory effect shows up during the EPSP TI inhibition, while there are both inhibitory and excitatory impacts on the EPSP peak and its RR.
Observations of an Inertial Peak in the Intrinsic Wind Spectrum Shifted by Rotation in the Antarctic Vortex
Authors: Gelinas L. J. , Walterscheid R. L. , Mechoso C. R. , Schubert G.
Published: September 2012

Spectral analyses of time series of zonal winds derived from locations of balloons drifting in the Southern Hemisphere polar vortex during the Vorcore campaign of the Stratéole program reveal a peak with a frequency near 0.10 h−1, more than 25% higher than the inertial frequency at locations along the trajectories. Using balloon data and values of relative vorticity evaluated from the Modern Era Retrospective-Analyses for Research and Applications (MERRA), the authors find that the spectral peak near 0.10 h−1 can be interpreted as being due to inertial waves propagating inside the Antarctic polar vortex. In support of this claim, the authors examine the way in which the low-frequency part of the gravity wave spectrum sampled by the balloons is shifted because of effects of the background flow vorticity. Locally, the background flow can be expressed as the sum of solid-body rotation and shear. This study demonstrates that while pure solid-body rotation gives an effective inertial frequency equal to the absolute vorticity, the latter gives an effective inertial frequency that varies, depending on the direction of wave propagation, between limits defined by the absolute vorticity plus or minus half of the background relative vorticity.
Circulation Response to Eurasian versus North American Anomalous Snow Scenarios in the Northern Hemisphere with an AGCM Coupled to a Slab Ocean Model
Authors: Henderson Gina R. , Leathers Daniel J. , Hanson Brian
Published: September 2012

The difference between snow-covered and snow-free conditions is the most climatically significant natural seasonal change the land surface can experience. Most GCM studies investigating snow–atmosphere interactions have focused on impacts of Eurasian snow anomalies caused by the magnitude of snow mass, while North American snow has been shown to have a weaker relationship with downstream climate. Experiment design of recent snow–atmosphere interactions studies has been limited to atmosphere-only models, with sea surface temperature (SST) and sea ice extent represented as boundary conditions. The authors explore the circulation response to anomalous snow scenarios, for both North America and Eurasia, using a slab ocean model. Surface response include significant SST cooling directly downstream of each individual forcing region in addition to upstream centers of remote cooling under maximum snow conditions. Atmospheric response to anomalous snow conditions is consistent through multiple levels in the lower troposphere under maximum snow conditions throughout much of the midlatitudes in both experiments during early winter. Areas of strengthened midtropospheric eddy kinetic energy correlate well with steep geopotential height gradient differences and increased zonal wind at 250 hPa over the western Pacific. Both experiments show similar atmospheric response pathways; however, circulation response to maximum Eurasian snow is focused downstream in early winter, whereas upstream response is particularly evident from the North American experiment. This paper focuses on differences as a result of Eurasian versus North American snow forcing in atmospheric circulation response using an AGCM with a slab ocean model.
ENSO-Like and ENSO-Induced Tropical Pacific Decadal Variability in CGCMs
Authors: Choi Jung , An Soon-Il , Yeh Sang-Wook , Yu Jin-Yi
Published: September 2012

Outputs from coupled general circulation models (CGCMs) are used in examining tropical Pacific decadal variability (TPDV) and their relationships with El Niño–Southern Oscillation (ENSO). Herein TPDV is classified as either ENSO-induced TPDV (EIT) or ENSO-like TPDV (ELT), based on their correlations with a decadal modulation index of ENSO amplitude and spatial pattern. EIT is identified by the leading EOF mode of the low-pass filtered equatorial subsurface temperature anomalies and is highly correlated with the decadal ENSO modulation index. This mode is characterized by an east–west dipole structure along the equator. ELT is usually defined by the first EOF mode of subsurface temperature, of which the spatial structure is similar to ENSO. Generally, this mode is insignificantly correlated with the decadal modulation of ENSO. EIT closely interacts with the residuals induced by ENSO asymmetries, both of which show similar spatial structures. On the other hand, ELT is controlled by slowly varying ocean adjustments analogous to a recharge oscillator of ENSO. Both types of TPDV have similar spectral peaks on a decadal-to-interdecadal time scale. Interestingly, the variances of both types of TPDV depend on the strength of connection between El Niño–La Niña residuals and EIT, such that the strong two-way feedback between them enhances EIT and reduces ELT. The strength of the two-way feedback is also related to ENSO variability. The flavors of El Niño–La Niña with respect to changes in the tropical Pacific mean state tend to be well simulated when ENSO variability is larger in CGCMs. As a result, stronger ENSO variability leads to intensified interactive feedback between ENSO residuals and enhanced EIT in CGCMs.
On the Dynamical Causes of Variability in the Rain-Shadow Effect: A Case Study of the Washington Cascades
Authors: Siler Nicholas , Roe Gerard , Durran Dale
Published: September 2012

Washington State’s Cascade Mountains exhibit a strong orographic rain shadow, with much wetter western slopes than eastern slopes due to prevailing westerly flow during the winter storm season. There is significant interannual variability in the magnitude of this rain-shadow effect, however, which has important consequences for water resource management, especially where water is a critically limited resource east of the crest. Here the influence of the large-scale circulation on the Cascade rain shadow is investigated using observations from the Snowfall Telemetry (SNOTEL) monitoring network, supplemented by stream gauge measurements. Two orthogonal indices are introduced as a basis set for representing variability in wintertime Cascade precipitation. First, the total precipitation index is a measure of regionwide precipitation and explains the majority of the variance in wintertime precipitation everywhere. Second, the rain-shadow index is a measure of the east–west precipitation gradient. It explains up to 31% of the variance west and east of the crest. A significant correlation is found between the winter-mean rain shadow and ENSO, with weak (strong) rain shadows associated with El Niño (La Niña). The analysis is supported by streamflow data from eastern and western watersheds. A preliminary review of individual storms suggests that the strongest rain shadows are associated with warm-sector events, while the weakest rain shadows occur during warm-frontal passages. This is consistent with known changes in storm tracks associated with ENSO, and a variety of mechanisms likely contribute.
Anarchism as the Contemporary Spirit of Anti-Capitalism? A Critical Survey of Recent Debates
Authors: el-Ojeili Chamsy
Published: September 2012

In the past decade or so, a sizable literature on anarchism has appeared. It has often been attached to the newer movements associated with alternative globalization and with post-modern theoretical currents. This literature is of significant interest for those working within the areas of social and political theory, globalization studies, and social movements. This article critically surveys the literature. It starts by examining the devices used to make the case for a renewed interest in anarchism, and traces the broad lines of historical anarchism. It then moves to explore the affinities between anarchism and the alternative globalization movement, and, at greater length, those between anarchism and contemporary theoretical issues. Critical of some of the post-modern excesses in this literature, and of the too sharp divide between Marxism and anarchism, this reconfigured anarchism is of great relevance. The article closes with some modest suggestions for further exploration in this work.
Improvement in the clinical practicability of roentgen stereophotogrammetric analysis (RSA): Free from the use of the dual X-ray equipment
Authors: Shih Kao-Shang , Lee Chian-Her , Syu Ci-Bin , Lai Jiing-Yih , Chen Kuo-Jen , Lin Shang-Chih
Published: September 2012

After total knee replacement, the monitoring of the prosthetic performance is often done by roentgenographic examination. However, the two-dimensional (2D) roentgen images only provide information about the projection onto the anteroposterior (AP) and mediolateral (ML) planes. Historically, the model-based roentgen stereophotogrammetric analysis (RSA) technique has been developed to predict the spatial relationship between prostheses by iteratively comparing the projective data for the prosthetic models and the roentgen images. During examination, the prosthetic poses should be stationary. This should be ensured, either by the use of dual synchronized X-ray equipment or by the use of a specific posture. In practice, these methods are uncommon or technically inconvenient during follow-up examination. This study aims to develop a rotation platform to improve the clinical applicability of the model-based RSA technique. The rotation platform allows the patient to assume a weight-bearing posture, while being steadily rotated so that both AP and ML knee images can be obtained. This study uses X-ray equipment with a single source and flat panel detectors (FPDs). Four tests are conducted to evaluate the quality of the FPD images, steadiness of the rotation platform, and accuracy of the RSA results. The results show that the distortion-induced error of the FPD image is quite minor, and the prosthetic size can be cautiously calibrated by means of the scale ball(s). The rotation platform should be placed closer to the FPD and orthogonal to the projection axis of the X-ray source. Image overlap of the prostheses can be avoided by adjusting both X-ray source and knee posture. The device-induced problems associated with the rotation platform include the steadiness of the platform operation and the balance of the rotated subject. Sawbone tests demonstrate that the outline error, due to the platform, is of the order of the image resolution (= 0.145 mm). In conclusion, the rotation platform with steady rotation, a knee support, and a handle can serve as an alternative method to take prosthetic images, without the loss in accuracy associated with the RSA method.
Modeling coding-sequence evolution within the context of residue solvent accessibility
Authors: Scherrer Michael P , Meyer Austin G , Wilke Claus O
Published: September 2012

Background

Protein structure mediates site-specific patterns of sequence divergence. In particular, residues in the core of a protein (solvent-inaccessible residues) tend to be more evolutionarily conserved than residues on the surface (solvent-accessible residues).

Results

Here, we present a model of sequence evolution that explicitly accounts for the relative solvent accessibility of each residue in a protein. Our model is a variant of the Goldman-Yang 1994 (GY94) model in which all model parameters can be functions of the relative solvent accessibility (RSA) of a residue. We apply this model to a data set comprised of nearly 600 yeast genes, and find that an evolutionary-rate ratioωthat varies linearly with RSA provides a better model fit than an RSA-independentωor anωthat is estimated separately in individual RSA bins. We further show that the branch lengthtand the transition-transverion ratioκalso vary with RSA. The RSA-dependent GY94 model performs better than an RSA-dependent Muse-Gaut 1994 (MG94) model in which the synonymous and non-synonymous rates individually are linear functions of RSA. Finally, protein core size affects the slope of the linear relationship betweenωand RSA, and gene expression level affects both the intercept and the slope.

Conclusions

Structure-aware models of sequence evolution provide a significantly better fit than traditional models that neglect structure. The linear relationship betweenωand RSA implies that genes are better characterized by theirωslope and intercept than by just their meanω.