bluuebunny/crossref_metadata_2025 huggingface dataset:
Monthly macroalgal surveys reveal a diverse and dynamic community in an urban intertidal zone.
Authors: Schenk Siobhan , Supratya Varoon , Martone Patrick T , Parfrey Laura Wegener
Published: February 2025

Baseline data are critical to measuring how communities shift in response to climate change and anthropogenic activity. However, baseline macroalgal biodiversity data are lacking for many areas of British Columbia, particularly at a high temporal resolution over years. This presents an obstacle for measuring how communities change in response to shifting average conditions or after extreme events such as the 2021 heat dome. To increase baseline macroalgae biodiversity data in British Columbia, we established a monthly transect-based macroalgal survey in 2021 at an urban intertidal site with a low-cost and easily replicable survey protocol, in addition to publishing a previously unpublished 1983-1984 historical dataset of the same area. Over 35 months we have recorded 61 taxa of macroalgae, including the canopy forming kelp Nereocystis luetkeana and the introduced fucoid Sargassum muticum. Surveying throughout the year at regular intervals has revealed large-scale seasonal shifts in macroalgal community composition, the timing of kelp recruitment, and a decrease in abundance of rockweed Fucus distichus over multiple years. Our publicly accessible data and protocol constitute the most comprehensive survey of intertidal macroalgal diversity in Burrard Inlet, illustrating how simple surveying methods can provide high resolution records of macroalgae biodiversity, particularly in accessible urban environments.
BEVA2.0: Modular Assembly of Golden Gate-Compatible Vectors with Expanded Utility for Genetic Engineering
Authors: Geddes Barney , Williamson Riley , Schumacher Jake D. D. , Ardi Ahmad , Levin Garrett , Červenka Emily , Huang Rui , diCenzo George C.
Published: February 2025

This expansion for the modular vector assembly platform BEVA (Bacterial Expression Vector Archive) introduces 11 new BEVA parts including two new cloning site variants, two new antibiotic resistance modules, three new origins of replication, and four new accessary modules. As a result, the modular system is now doubled in size and expanded in its capacity to produce diverse replicating plasmids. Furthermore, it is now amenable to genetic engineering methods involving genome-manipulation of target strains through deletions or integrations. In addition to introducing the new modules, we provide several BEVA-derived Golden Gate cloning plasmids that are used to validate parts and that may be useful for genetic engineering of proteobacteria and other bacteria. We also introduce new parts to allow compatibility with the CIDAR MoClo parts libraries.
Supersymmetric $$AdS_6$$ black holes from ISO(3)$$\times $$U(1) F(4) gauged supergravity
Authors: Karndumri Parinya
Published: February 2025

We study supersymmetric

$$AdS_6$$

A
d

S
6

black holes from matter-coupled F(4) gauged supergravity coupled to four vector multiplets with

$$ISO(3)\times U(1)$$

I
S
O
(
3
)
×
U
(
1
)

gauge group. This gauged supergravity admits a maximally supersymmetric

$$AdS_6$$

A
d

S
6

vacuum with

$$SO(3)\subset ISO(3)$$

S
O
(
3
)
⊂
I
S
O
(
3
)

symmetry. We find a number of new supersymmetric

$$AdS_2\times \mathcal {M}_4$$

A
d

S
2

×

M
4

solutions by performing topological twists along

$$\mathcal {M}_4$$

M
4

. For

$$\mathcal {M}_4$$

M
4

being a product of two Riemann surfaces

$$\Sigma \times \widetilde{\Sigma }$$

Σ
×

Σ
~

, we perform a twist by

$$SO(2)\times U(1)$$

S
O
(
2
)
×
U
(
1
)

gauge fields and find

$$AdS_2\times \Sigma \times \widetilde{\Sigma }$$

A
d

S
2

×
Σ
×

Σ
~

solutions for at least one of the Riemann surface being negatively curved. For

$$\mathcal {M}_4$$

M
4

being a Kahler four-cycle

$$\mathcal {M}_{\text {K}4}$$

M

K
4

, a twist by

$$SO(2)\times U(1)$$

S
O
(
2
)
×
U
(
1
)

gauge fields leads to

$$AdS_2\times \mathcal {M}_{\text {K}4}^-$$

A
d

S
2

×

M

K
4

-

solutions for negatively curved

$$\mathcal {M}_{\text {K}4}$$

M

K
4

. Finally, performing a twist by turning on SO(3) gauge fields in the case of

$$\mathcal {M}_4$$

M
4

being a Cayley four-cycle

$$\mathcal {M}_{\text {C}4}$$

M

C
4

also leads to

$$AdS_2\times \mathcal {M}_{\text {C}4}^-$$

A
d

S
2

×

M

C
4

-

solutions for negatively curved

$$\mathcal {M}_{\text {C}4}$$

M

C
4

. We give numerical black hole solutions interpolating between all of these

$$AdS_2\times \mathcal {M}_4$$

A
d

S
2

×

M
4

near horizon geometries and the asymptotically locally

$$AdS_6$$

A
d

S
6

vacuum. It is also possible to uplift all of these solutions to type IIB theory via consistent truncations on

$$S^2\times \Sigma $$

S
2

×
Σ

leading to a new class of supersymmetric

$$AdS_2\times \mathcal {M}_4\times S^2\times \Sigma $$

A
d

S
2

×

M
4

×

S
2

×
Σ

solutions.
Voltage-gated calcium channel α2δ-1 subunit is involved in the regulation of glucose-stimulated GLP-1 secretion in mice
Authors: Yang Yuhan , Yamane Shunsuke , Harada Norio , Ikeguchi-Ogura Eri , Yamamoto Kana , Wada Naoki , Fauzi Muhammad , Murakami Takaaki , Yabe Daisuke , Hayashi Yoshitaka , Inagaki Nobuya
Published: February 2025

In this study, we establish high expression of the voltage-gated Ca2+ channel α2δ-1 subunit (CaVα2δ-1) subunit in enteroendocrine glucagon-like peptide-1 (GLP-1) producing cells and elucidate its role in GLP-1 secretion, providing a more detailed understanding of the mechanism of GLP-1 secretion.
Emerging roles of noncoding RNAs in cardiovascular pathophysiology
Authors: Abbas Malak , Gaye Amadou
Published: February 2025

This review comprehensively examines the diverse roles of noncoding RNAs (ncRNAs) in the pathogenesis and treatment of cardiovascular disease (CVD), focusing on microRNA (miRNA), long noncoding RNA (lncRNA), piwi-interacting RNA (piRNA), small-interfering RNA (siRNA), circular RNA (circRNA), and vesicle-associated RNAs. These ncRNAs are integral regulators of key cellular processes, including gene expression, inflammation, and fibrosis, and they hold great potential as both diagnostic biomarkers and therapeutic targets. The review highlights novel insights into how these RNA species, particularly miRNAs, lncRNAs, and piRNAs, contribute to various CVDs such as hypertension, atherosclerosis, and myocardial infarction. In addition, it explores the emerging role of extracellular vesicles (EVs) in intercellular communication and their therapeutic potential in cardiovascular health. The review underscores the need for continued research into ncRNAs and RNA-based therapies, with a focus on advancing delivery systems and expanding personalized medicine approaches to improve cardiovascular outcomes.
Renal Macro- and Microcirculatory Perturbations in Acute Kidney Injury and Chronic Kidney Disease Associated with Heart Failure and Cardiac Surgery
Authors: Trask-Marino Anton L. , Marino Bruno , Lancefield Terase F. , See Emily J. , May Clive N. , Booth Lindsea C. , Raman Jai , Lankadeva Yugeesh R.
Published: February 2025

Chronic kidney disease (CKD) affects 50% of patients with heart failure. The pathophysiology of CKD in heart failure is proposed to be driven by macrocirculatory hemodynamic changes, including reduced cardiac output and elevated central venous pressure. However, our understanding of the renal microcirculation in heart failure and CKD remains limited. This is largely due to the lack of non-invasive techniques to assess the renal microcirculation in patients. Moreover, there is a lack of clinically relevant animal models of heart failure and CKD to advance our understanding of the timing and magnitude of renal microcirculatory dysfunction. Patients with heart failure and CKD commonly require cardiac surgery with cardiopulmonary bypass (CPB) to improve their prognosis. However, acute kidney injury (AKI) is a frequent unresolved clinical complication in these patients. There is emerging evidence that renal microcirculatory dysfunction, characterized by renal medullary hypoperfusion and hypoxia, plays a critical role in the pathogenesis of cardiac surgery-associated AKI. In this review, we consolidate the preclinical and clinical evidence of renal macro- and microcirculatory perturbations in heart failure and cardiac surgery requiring CPB. We also examine emerging biomarkers and therapies that may improve health outcomes for this vulnerable patient population by targeting the renal microcirculation.
Shear Capacity Calculation Methods for Reinforced Concrete Structure
Authors: Li Qi , Chen Mengcheng , Li Yi , Zhang Mingyang
Published: February 2025

In recent years, there have been several instances of building collapse incidents resulting from shear failure. The complicated stress state and extremely nonlinear behavior seen in RC structures under shear loading are responsible for this phenomenon. Scholars and engineers worldwide have yet to propose a suitable model to address the issue correctly. Most computational approaches currently in use rely on semi‐empirical and semi‐theoretical formulations. The primary modes of shear transmission in cracked reinforced concrete include shear stress inside the uncracked concrete region, aggregate interlock, dowel action, stirrup action, and tensile residual stress. The shear force calculation is contingent upon the choice of the shear transfer model. This study comprehensively overviews the current shear calculation techniques and their respective applications. The pros and cons of these techniques were thoroughly examined. The present study investigated the suitability of these shear calculation formulas for RC structures subjected to intricate natural conditions, mechanical stress, and high‐strength and high‐performance concrete structures.
Effects of Dietary Vitamin A on the Growth Performance, Nonspecific Immune Response, Shell Microbiota and Red Spotted Disease Resistance of Juvenile Sea Urchin (Strongylocentrotus intermedius)
Authors: Gou Dan , Xu Rujian , Liu Haijing , Gong Panke , Di Weixiao , Zuo Huinan , Ding Jun , Chang Yaqing , Zuo Rantao
Published: February 2025

A 114‐day feeding trial was used to investigate the influence of vitamin A (VA) on growth performance, nonspecific immune responses and shell microbiota in juvenile sea urchin (Strongylocentrotus intermedius). Graded levels of VA (0, 4000, 8000, 16,000, 32,000 and 64,000 IU/kg) were added to make six experimental feeds. Each feed was allocated to three parallel tanks of sea urchins (initial weight 0.87 ± 0.05 g and initial test diameter 1.83 ± 0.57 mm). The data revealed that the weight gain rate (WGR) and gonadosomatic index (GSI) rose markedly as VA addition level increased from 0 to 4000 IU/kg and then reached a plateau with further increase of dietary VA levels. As VA addition level increased, nonspecific immune response of S. intermedius first increased and then decreased, with those fed diets with relatively higher addition of VA (32,000 IU/kg) exhibiting significantly greater phagocytic activity (PA) and acid phosphatase (ACP) activities, as well as upregulated expression of several immune‐related genes such as tumour necrosis factor α (TNF-α), antimicrobial peptides (AMPs), toll‐like receptors (TLRs) and lysozyme (LYZ). The abundance of Firmicutes, Bacteroidota, Bacteroides and Faecalibacterium increased, but that of Proteobacteria and Leucothrix decreased in the shell of S. intermedius as VA addition level increased. The percentage of sea urchins with severe red spotted disease decreased from 64.44% to13.33% as VA addition level increased to 32,000 IU/kg and subsequently increased to 42.22% with further increase of VA addition level. On the contrary, the percentage of sea urchins with mild red spotted disease increased from13.33% to 55.55% as VA addition level increased to 32,000 IU/kg and subsequently decreased to 31.11% with further increase of VA addition level. These results demonstrated that a low addition level of VA (4000 IU/kg) can help S. intermedius achieve ideal growth performance. However, relatively higher addition levels of VA (32,000 IU/kg) enhanced nonspecific immunity and red spotted disease resistance of S. intermedius, which could be accomplished by promoting immune gene expression and optimizing the shell microbiota composition.
A Rare Case of Atherosclerosis of the Angular Artery Manifested as a Nodular Lesion on the Skin and Review of the Literature
Authors: Nazário Wender Rodrigues , Novais Marcondes Pedro Souza , Costa Anaíra Ribeiro Guedes Fonseca , de Oliveira Santos Débora , de Paula Douglas Magallhães , Cardoso Sérgio Vitorino , de Faria Paulo Rogério
Published: February 2025

Atherosclerosis is an age‐related disease affecting the tunica intima of large and medium‐sized arteries. Its occurrence in extracranial head and neck arteries is rare, with only five cases reported. This study is aimed at reporting the sixth case of atherosclerosis of the angular artery, a branch of the facial artery, in an elderly male patient with a history of hypertension. Morphological characterization using special stains and a literature review are included. Clinically, a painless, nodular lesion measuring 1.2 cm was observed at the right labial commissure. The lesion was excised, and atherosclerosis of the angular artery was diagnosed. After 6 months, there was no recurrence or other arterial issues. Despite its rarity in the oral and maxillofacial region, dental clinicians must be aware of this condition and its clinical manifestations, especially in elderly patients with chronic systemic diseases, to establish an accurate diagnosis.
Prediction of Excess Air Ratio Through Deep Neural Network–Based Multidimensional Analysis of OH∗ Radical Intensity and Fuel Pressure in Flame
Authors: So Byeongchan , Kwon Minjun , Kim Jongwon , Kim Sewon , So Hongyun
Published: February 2025

This study proposes a deep neural network (DNN)–based regression model for predicting the excess air ratio, which is a critical indicator for optimizing combustion efficiency and minimizing harmful emissions in industrial combustion systems. The chemiluminescence signals of the OH∗ radicals and fuel pressure were used as the input features for the prediction model. To evaluate the effect of the multidimensional input, Case 1, with only the OH∗ radical signal as a single input, was compared with Case 2, with the OH∗ radical signal and fuel pressure as the inputs. The results showed that the Case 2 model reduced the mean absolute error (MAE), mean relative error (MRE), and root mean squared error (RMSE) by approximately 40.71%, 41.85%, and 19.69%, respectively, compared to Case 1, and the average relative prediction error rate was also 2.25% lower. These results demonstrate the potential for improving the accuracy and generalization ability of the model by incorporating multidimensional input features. Therefore, DNN models using multidimensional inputs can contribute to the design and implementation of combustion control systems to optimize the combustion efficiency and reduce harmful emissions in industrial combustion systems by predicting the excess air ratio.
Association of Sleep Duration With Serum Estradiol Concentrations Among American Men and Women: Evidence From NHANES 2013–2016
Authors: Zhu Zhisheng , Wu Shiquan , Lin Xingong , Wang Chaoyang , Zhou Xianying
Published: February 2025

Objective: To evaluate the association between sleep duration with serum estradiol concentrations and its variation by sex and age in American adults.

Methods: Data were analyzed for 5406 men and women (≥ 20 years old) who participated in the cycles of the National Health and Nutrition Examination Survey 2013–2016, a cross‐sectional study. Total estradiol (pg/mL) was measured and categorized (low, normal, and high) based on the NHANES protocol. Sleep duration was classified as ≤ 6, 6–9, and ≥ 9 h. Weighted multivariable adjusted and multinomial logistic regression models were conducted to assess these associations.

Results: Our multivariable multinomial logistic regression analysis revealed no significant associations between sleep duration and serum estradiol concentrations among both American men and women. Specifically, comparisons of sleep durations (≤ 6 and ≥ 9 h) to the reference group (6–9 h) across various age categories showed odds ratios for low and high estradiol concentrations that remained statistically nonsignificant in fully adjusted models. These findings suggest that, unlike previous studies linking sleep duration with variations in other hormones, estradiol concentrations do not appear to be significantly affected by differences in sleep duration in either sex across all age groups studied.

Conclusion: The lack of significant associations between sleep duration and serum estradiol concentrations indicates that sleep duration may not influence estradiol levels in the general population of American men and women. These results underscore the importance of continued research into how sleep influences hormonal balance. However, it is important to note that the NHANES data we used are from a cross‐sectional study, which cannot establish a causal relationship between sleep duration and serum estradiol. Future studies should investigate additional factors, such as genetic predispositions, lifestyle habits, and environmental influences, that may modulate the relationship between sleep and hormone levels.
Comparative Analysis of Total Antioxidant Status (TAS) of Ready‐to‐Consume Turkish Coffees With Different Flavoring Agents
Authors: Ağagündüz Duygu , Ağbal Zilal , Sarikaya Dilara , Sağit Belgin , Şahin Teslime Özge
Published: February 2025

The production and consumption of both traditional and flavored Turkish coffee varieties are widespread in several countries, including Turkey. However, the health effects of flavorings used to enhance the flavor of commercially flavored coffees remain unclear. The aim of this study was to evaluate the total antioxidant status (TAS), a biomarker of health, in plain and commercially flavored Turkish coffees (blackberry, wild strawberry, hazelnut, vanilla, rose Turkish delight, and chocolate) commonly consumed by the Turkish population. In this study, a total of 24 different coffee samples of 6 different brands with plain, blackberry, wild strawberry, hazelnut, vanilla, rose delight, and chocolate flavors were examined. TAS analysis of the samples was performed with a commercial test kit based on (2,2′‐azino‐di‐(3‐ethylbenzthiazoline sulphonate) (ABTS) radical scavenging activity, and the results were expressed in mmol Trolox equivalent (TE)/L. The results showed that blackberry‐flavored(3.14 ± 0.07 mmol TE/L) and vanilla‐flavored (3.13 ± 0.07 mmol TE/L) Turkish coffees exhibited lower TAS levels compared to plain Turkish coffee (3.23 ± 0.01 mmol TE/L) and coffees flavored with wild strawberry (3.24 ± 0.04 mmol TE/L), chocolate (3.22 ± 0.01 mmol TE/L), hazelnut (3.22 ± 0.01 mmol TE/L), and rose delight (3.23 ± 0.01 mmol TE/L) (p < 0.05). In conclusion, individuals who want to diversify their Turkish coffee consumption while maintaining or improving their health may prefer wild strawberry‐, chocolate‐, hazelnut‐, and rose delight–flavored coffees over blackberry‐ and vanilla‐flavored coffees. This study highlights the potential health benefits of certain flavored coffees as an alternative to traditional plain Turkish coffee.
Coweakly Uniserial Modules and Rings Whose (2‐Generated) Modules Are Coweakly Uniserial
Authors: Oladghobad M. M. , Beyranvand R.
Published: February 2025

A module is called weakly uniserial if for any two its submodules at least one of them is embedded in the other. This is a nontrivial generalization of uniserial modules and rings. Here, we introduce and study the dual of this concept. In fact, an R‐module M is called coweakly uniserial if for any submodules N, K of M, HomR(M/N, M/K) or HomR(M/K, M/N) contains a surjective element. In this paper, in addition to presenting the properties of this concept, we show that a ring R is homogeneous semisimple if and only if every (projective) right R‐module is coweakly uniserial. Also, in a semi‐Artinian ring R, it is shown that if every 2‐generated right R‐module is coweakly uniserial, then R is a homogeneous semisimple ring. Then, we prove that has no coweakly uniserial subgroups. Among applications of our results, we classify quasi‐continuous, quasi‐injective, and uniform abelian groups that are coweakly uniserial.
Structure of Overlying Rock Strata and Advanced Abutment Stress Evolution in Deep Filling Mining
Authors: Chen Yang , Sun Xikui , Gu Peng , Li Guoying , Fan Deyuan , Dong Chunyu , Zhang Yu , Wang Hao , Liu Chuancheng , Liu Xuesheng
Published: February 2025

Filling mining is one of the feasible methods to prevent rock burst in coal mining, which is a research significance topic in the field. In this paper, we first described the filling mining effect based on “equivalent mining height” and analyzed the evolution of overlying rock strata. It was found that time‐space hysteresis occurred during the movement of overlying rock strata in filled working face. On this basis, the mechanical relationship of dynamic transformation among “filling rate–strata movement–abutment stress” was obtained by analyzing the coal stress characteristics. Then, an advanced abutment stress estimation model of filled working face in deep coal mine was established. Finally, the C5301 working face of Yunhe coal mine was taken as the engineering background, and example calculation and field monitoring were carried out. The results showed that the influence range of the advanced abutment stress is 91–97 m, and the peak value is 41.1 MPa, which is 21–50 m away from the working face. In addition, large pressure steps and long interval time were the characteristics of time‐space hysteresis in the filled working face. This study could provide guidance and reference for deep filling mining under the same or similar conditions.
Supplementary Figure Legends from Intra- and Extrahepatic Cholangiocarcinomas Display Differing Sensitivities to NK Cell Lysis and Modulate NK Cell Function through Shared and Distinct Pathways
Authors: Nguyen Ngan , Henrich Ian C.
Published: February 2025

Supplementary Figure Legends
Supplemental Figure 3 from Intra- and Extrahepatic Cholangiocarcinomas Display Differing Sensitivities to NK Cell Lysis and Modulate NK Cell Function through Shared and Distinct Pathways
Authors: Nguyen Ngan , Henrich Ian C.
Published: February 2025

Figure S3. Effect of CCA co-culture on other NK receptors.
Prediagnostic Plasma Metabolites Are Associated with Incident Hepatocellular Carcinoma: A Prospective Analysis
Authors: Wilechansky Robert M. , Challa Prasanna K. , Han Xijing , Hua Xinwei , Manning Alisa K. , Corey Kathleen E. , Chung Raymond T. , Zheng Wei , Chan Andrew T. , Simon Tracey G.
Published: February 2025

Despite increasing incidence of hepatocellular carcinoma (HCC) in vulnerable populations, accurate early detection tools are lacking. We aimed to investigate the associations between prediagnostic plasma metabolites and incident HCC in a diverse population. In a prospective, nested case–control study within the Southern Community Cohort Study, we conducted prediagnostic LC/MS metabolomic profiling in 150 incident HCC cases (median time to diagnosis, 7.9 years) and 100 controls with cirrhosis. Logistic regression assessed metabolite associations with HCC risk. Metabolite set enrichment analysis identified enriched pathways, and a random forest classifier was used for risk classification models. Candidate metabolites were validated in the UK Biobank (N = 12 incident HCC cases and 24 cirrhosis controls). In logistic regression analysis, seven metabolites were associated with incident HCC (MeffP < 0.0004), including N-acetylmethionine (OR = 0.46; 95% confidence interval, 0.31–0.66). Multiple pathways were enriched in HCC, including histidine and CoA metabolism (FDR P < 0.001). The random forest classifier identified 10 metabolites for inclusion in HCC risk classification models, which improved HCC risk classification compared with clinical covariates alone (AUC = 0.66 for covariates vs. 0.88 for 10 metabolites plus covariates; P < 0.0001). Findings were consistent in the UK Biobank (AUC = 0.72 for covariates vs. 0.88 for four analogous metabolites plus covariates; P = 0.04), assessed via nuclear magnetic resonance spectroscopy. Prediagnostic metabolites, primarily amino acid and sphingolipid derivatives, are associated with HCC risk and improve HCC risk classification beyond clinical covariates. These metabolite profiles, detectable years before diagnosis, could serve as early biomarkers for HCC detection and risk stratification if validated in larger studies.

Prevention Relevance: Our findings support the need for larger prospective studies examining the role of prediagnostic plasma metabolomics for the preventive management of HCC in diverse patients across multiple etiologies of liver disease. This approach could improve HCC care by identifying metabolic changes years before diagnosis, potentially enhancing screening and early detection practices.
Supplementary Table S6 from The UBA1–STUB1 Axis Mediates Cancer Immune Escape and Resistance to Checkpoint Blockade
Authors: Bao Yi , Cruz Gabriel , Zhang Yuping , Qiao Yuanyuan , Mannan Rahul , Hu Jing , Yang Fan , Gondal Mahnoor , Shahine Miriam , Kang Sarah , Mahapatra Somnath , Chu Alec , Choi Jae Eun , Yu Jiali , Lin Heng , Miner Stephanie J. , Robinson Dan R. , Wu Yi-Mi , Zheng Yang , Cao Xuhong , Su Fengyun , Wang Rui , Hosseini Noshad , Cieslik Marcin , Kryczek Ilona , Vaishampayan Ulka , Zou Weiping , Chinnaiyan Arul M.
Published: February 2025

Supplementary Table S6. Data from the CRISPR screen in Myc-CaP cells treated with TAK-243 + IFN-β.
Supplementary Figure S5 from The UBA1–STUB1 Axis Mediates Cancer Immune Escape and Resistance to Checkpoint Blockade
Authors: Bao Yi , Cruz Gabriel , Zhang Yuping , Qiao Yuanyuan , Mannan Rahul , Hu Jing , Yang Fan , Gondal Mahnoor , Shahine Miriam , Kang Sarah , Mahapatra Somnath , Chu Alec , Choi Jae Eun , Yu Jiali , Lin Heng , Miner Stephanie J. , Robinson Dan R. , Wu Yi-Mi , Zheng Yang , Cao Xuhong , Su Fengyun , Wang Rui , Hosseini Noshad , Cieslik Marcin , Kryczek Ilona , Vaishampayan Ulka , Zou Weiping , Chinnaiyan Arul M.
Published: February 2025

Supplementary Figure S5. UBA1 inactivation upregulates interferon signaling.
Supplementary Figure S2 from The UBA1–STUB1 Axis Mediates Cancer Immune Escape and Resistance to Checkpoint Blockade
Authors: Bao Yi , Cruz Gabriel , Zhang Yuping , Qiao Yuanyuan , Mannan Rahul , Hu Jing , Yang Fan , Gondal Mahnoor , Shahine Miriam , Kang Sarah , Mahapatra Somnath , Chu Alec , Choi Jae Eun , Yu Jiali , Lin Heng , Miner Stephanie J. , Robinson Dan R. , Wu Yi-Mi , Zheng Yang , Cao Xuhong , Su Fengyun , Wang Rui , Hosseini Noshad , Cieslik Marcin , Kryczek Ilona , Vaishampayan Ulka , Zou Weiping , Chinnaiyan Arul M.
Published: February 2025

Supplementary Figure S2. UBA1 promotes tumor growth by mediating immune escape.
Supplementary Table 10 from CTR-FAPI PET Enables Precision Management of Medullary Thyroid Carcinoma
Authors: Kong Ziren , Li Zhu , Cui Xi-Yang , Wang Jian , Xu Mengxin , Liu Yang , Chen Junyi , Ni Song , Zhang Zongmin , Fan Xiaowei , Huang Jiazhao , Lin Yansong , Sun Yuning , He Yuqin , Lin Xinfeng , Meng Tianyu , Li Han , Song Yixuan , Peng Boshizhang , An Changming , Gao Chenyan , Li Nan , Liu Chen , Zhu Yiming , Yang Zhi , Liu Zhibo , Liu Shaoyan
Published: February 2025

Presurgical imaging identification of the 60 lesions
Supplementary Table S3 from Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma
Authors: Bischoff Megan E. , Shamsaei Behrouz , Yang Juechen , Secic Dina , Vemuri Bhargav , Reisz Julie A. , D’Alessandro Angelo , Bartolacci Caterina , Adamczak Rafal , Schmidt Lucas , Wang Jiang , Martines Amelia , Venkat Jahnavi , Tcheuyap Vanina Toffessi , Biesiada Jacek , Behrmann Catherine A. , Vest Katherine E. , Brugarolas James , Scaglioni Pier Paolo , Plas David R. , Patra Krushna C. , Gulati Shuchi , Landero Figueroa Julio A. , Meller Jarek , Cunningham John T. , Czyzyk-Krzeska Maria F.
Published: February 2025

Cardiolipins speciation
Supplementary Table S1 from Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma
Authors: Bischoff Megan E. , Shamsaei Behrouz , Yang Juechen , Secic Dina , Vemuri Bhargav , Reisz Julie A. , D’Alessandro Angelo , Bartolacci Caterina , Adamczak Rafal , Schmidt Lucas , Wang Jiang , Martines Amelia , Venkat Jahnavi , Tcheuyap Vanina Toffessi , Biesiada Jacek , Behrmann Catherine A. , Vest Katherine E. , Brugarolas James , Scaglioni Pier Paolo , Plas David R. , Patra Krushna C. , Gulati Shuchi , Landero Figueroa Julio A. , Meller Jarek , Cunningham John T. , Czyzyk-Krzeska Maria F.
Published: February 2025

Differential gene expression in CuHi and CuLo 786-O cells.
Supplementary Figure S5 from Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma
Authors: Bischoff Megan E. , Shamsaei Behrouz , Yang Juechen , Secic Dina , Vemuri Bhargav , Reisz Julie A. , D’Alessandro Angelo , Bartolacci Caterina , Adamczak Rafal , Schmidt Lucas , Wang Jiang , Martines Amelia , Venkat Jahnavi , Tcheuyap Vanina Toffessi , Biesiada Jacek , Behrmann Catherine A. , Vest Katherine E. , Brugarolas James , Scaglioni Pier Paolo , Plas David R. , Patra Krushna C. , Gulati Shuchi , Landero Figueroa Julio A. , Meller Jarek , Cunningham John T. , Czyzyk-Krzeska Maria F.
Published: February 2025

Expression of SLC25A40 glutathione transporter in RCC cells.
Knudson’s “Two-Hit” Hypothesis and Cancer Predisposition: A Bit More Complicated but Still Going Strong
Authors: Brzezinski Jack J. , Malkin David
Published: February 2025

This study by Treger and colleagues is a comprehensive evaluation of the genome and epigenome of tumors and constitutional tissue from children with Wilms tumor predisposition syndromes that demonstrates that the molecular features of Wilms tumors are dependent on the constitutional milieu of the patient in which they develop.

See related article by Treger et al., p. 286
Hidden in Plain Sight: Clinical Imaging of the Tumor Microenvironment with PET
Authors: Witney Timothy H.
Published: February 2025

PET imaging enables the spatiotemporal assessment of tumor biomarkers. In this issue, Kong and colleagues describe the clinical PET imaging of tumor-associated fibroblasts, which improved the diagnostic accuracy and management of a subset of patients with medullary thyroid carcinoma.

See related article by Kong et al., p. 316
THREE FRACTION EXTERNAL BEAM RADIOTHERAPY IN PATIENTS WITH EARLY BREAST CANCER
Authors: Yadav Budhi Singh , Appakattu kuzhi Habeeb Kudukkil , Dahiya Divya , Rathod Monsu , Singh Arun Oinam , Singh Gaganpreet
Published: February 2025

Background
Patients with early breast cancer (EBC) can be treated with accelerated partial breast irradiation (APBI). The objective of this study was to determine to see if a three fraction APBI schedule is feasible for these patients.
Material & Methods
This single-institutional, phase 2 study included patients with age ≥ 45 years, tumor size <3.5 cm, negative nodes, negative surgical margins and unicentric tumours. Patients were treated with 3D-CRT technique with a dose of 22.5 Gy in 3 fractions on 3 consecutive days. The primary end point of the study was cosmesis as assessed by physicians at 3-years. Secondary end points were cosmesis as assessed by patients, dosimetry, acute and late toxicities, disease-free and overall survival.
Results
From October 2019 to October 2022, 49 patients were treated. Mean age of the patients was 55 years (range 40 - 75). At 3 years, physician- and patient-reported cosmetic results were excellent/good/fair in 33 (67%)/14 (29%)/2 (4%) of patients and in 29 (59%)/16 (33%)/4 (8%) of patients, respectively. Acute dermatitis grades 1 and 2 were observed in 14 (29%) and 3 (6%) of patients, respectively. At 6 months, breast edema, skin induration, subcutaneous fibrosis, pain and pigmentation were observed in 3 (6%), 6 (12%), 3 (6%), 2 (4%) and 8 (16%) of patients, respectively. At 3 years, 1 (2%) patient had mild pain; no breast edema, skin induration, subcutaneous fibrosis or pigmentation was observed. At a median follow up of 39 months (range 24-60), there were no local/regional recurrences. One patient developed distant metastases in the bones, liver, and lungs at 46 months. All patients were alive at last follow-up.

Conclusions
In patients with EBC, three fraction APBI is feasible. Physicians- and patients-reported cosmesis was excellent/good in 96% and 92% of patients, respectively. Late effects were only grade 1.
Molecular tissue profiling in a clinically selected pancreatic cancer cohort
Authors: Hegenberg Stefanie , Germing Tobias , Schlageter Elena , Ekmekciu Ira , Christmann Jens , Tannapfel Andrea , Reinacher-Schick Anke
Published: February 2025

Introduction: Pancreatic ductal adenocarcinoma (PDAC) is a challenging malignancy and precision oncology treatment options may improve patient outcomes. Next-generation sequencing (NGS) is an established tool that enables multi-gene panel analysis. Methods: This NGS tissue-based analysis, conducted at a German pancreatic cancer center, included 128 patients between January 2016 and January 2021. Results: A targetable lesion was detected in 15.6% of patients. BRCA1/2 mutations were identified in 16 patients, of whom 13 had germline mutations; eight of these patients received Olaparib. In 41.4% of patients, homologous recombination repair (HRR) genes were affected. Two cases of ATP1B1-NRG1 fusions and seven cases of dMMR tumors were found, leading to individualized treatment. KRAS mutations were present in 70.3%, and TP53 mutations in 63.3%. A total of 80.5% of patients received platinum-based chemotherapy, predominantly FOLFIRINOX. Conclusion: The high frequency of targetable alterations in our cohort underscores upfront NGS testing in PDAC. Younger patients, those with a positive FH and KRAS WT patients should be of particular interest.
Temporal Dynamics and Representational Consequences of the Control of Processing Conflict between Visual Working Memory and Visual Perception
Authors: Teng Chunyue , Fulvio Jacqueline M. , Pietrelli Mattia , Jiang Jiefeng , Postle Bradley R.
Published: February 2025

Visual working memory (WM) extensively interacts with visual perception. When information between the two processes is in conflict, cognitive control can be recruited to effectively mitigate the resultant interference. The current study investigated the neural bases of the control of conflict between visual WM and visual perception. We recorded the EEG from 25 human participants (13 male) performing a dual task combining visual WM and tilt discrimination, the latter occurring during the WM delay. The congruity in orientation between the memorandum and the discriminandum was manipulated. Behavioral data were fitted to a reinforcement-learning model of cognitive control to derive trial-wise estimates of demand for preparatory and reflexive control, which were then used for EEG analyses. The level of preparatory control was associated with sustained frontal-midline theta activity preceding trial onset, as well as with the strength of the neural representation of the memorandum. Subsequently, discriminandum onset triggered a control prediction error signal that was reflected in a left frontal positivity. On trials when an incongruent discriminandum was not expected, reflexive control that scaled with the prediction error acted to suppress the neural representation of the discriminandum, producing below-baseline decoding of the discriminandum that, in turn, exerted a repulsive serial bias on WM recall on the subsequent trial. These results illustrate the flexible recruitment of two modes of control and how their dynamic interplay acts to mitigate interference between simultaneously processed perceptual and mnemonic representations.
Exploring Dynamic Brain Oscillations in Motor Imagery and Low-frequency Sound
Authors: Dupont William , Poulin-Charronnat Benedicte , Madden-Lombardi Carol , Jacquet Thomas , Pfister Philippe , Dos Anjos Typhanie , Lebon Florent
Published: February 2025

Although both motor imagery (MI) and low-frequency sound listening have independently been shown to modulate brain activity, the potential synergistic effects that may arise from their combined application remains unexplored. Any further modulation derived from this combination may be relevant for motor learning and rehabilitation. We probed neurophysiological activity during these two processes, measuring alpha and beta band power amplitude by means of EEG recordings. Twenty healthy volunteers were instructed to (i) explicitly imagine right finger flexion/extension movements in a kinaesthetic modality, (ii) listen to low-frequency sounds, (iii) imagine right finger movements while listening to low-frequency sounds, or (iv) stay at rest. We observed a bimodal distribution of alpha-band reactivity to the conditions, suggesting the presence of variability in brain activity across participants during both MI and low-frequency sound listening. One group of participants (12 individuals) displayed increased alpha power within contralateral sensorimotor and ipsilateral medial parieto-occipital regions during MI. Another group (eight individuals) exhibited a decrease in alpha and beta band power within sensorimotor areas. Interestingly, low-frequency sound listening elicited a similar pattern of brain activity within both groups. The combination of MI and sound listening did not result in additional changes in alpha and beta power amplitudes, regardless of group (groups based on individual alpha-band reactivity). Altogether, these findings shed significant insight into the brain activity and its variability generated during MI and low-frequency sound listening. The simultaneous engagement of MI and low-frequency sound listening did not further modulate alpha power amplitude, possibly due to concurrent cortical activation. It remains possible that sequential performance of these tasks could elicit additional modulation.
From semantic structure to conventionalization
Authors: Katzir Nicole , Cummins Chris
Published: February 2025

The present study investigates potential differences among 40 quantifier scales regarding measures that may indicate variations in conventionalization among different scales, i.e., the extent to which their terms are stored as scalemates in the mental lexicon. We derive these measures from two experiments and corpus data. In addition to exploring the relationships between these measures, we also investigate their correlation with scalar inference (SI) rates. We compare the quantifier scales to scales from two categories: highly and moderately conventionalized. Our findings indicate that quantifier scales pattern with moderately conventionalized scales in the experimental tasks. Additionally, we identify two significant predictors of SI rates that are not directly derived from the semantics of the quantifiers: confidence in an elicitation task and the observed/expected ratio of sentences in a corpus. Finally, we propose that the semantic and non-semantic features generally align, working together to reinforce the connection between the scalemates.
Exploring the pragmatics of autistic language
Authors: Rebecchi Kevin
Published: February 2025

This article examines the linguistic aspects of autism, focusing specifically on expressive and phatic traits, reevaluating the field of pragmatics. Inspired by the emphasis on inclusion and diverse modes of expression in the Reggio Emilia pedagogy, this study explores the applicability of Reggio Emilia’s pedagogical principles to autism research. The concept of “hundred languages” highlights the multitude of ways in which children can express themselves, think, communicate, and create. By prioritizing inclusive education and recognizing individual differences, the Reggio Emilia approach provides valuable insights for creating inclusive educational environments for autistic individuals. Furthermore, the concept of “hundred languages” aligns with the diverse modes of expression and communication observed in autistic individuals. Emphasis is placed on the pragmatic aspects of language, including social context and the management of similarities and differences in social interactions. This prompts an exploration of how autistic pragmatics may differ from that of non-autistic individuals. The dynamics of linguistic power, wherein individuals in positions of power use language and influence specific segments of the population, raise questions about the impact of power structures on autistic individuals and their language experiences. Moreover, historical descriptions of language in autism provide valuable insights. Observations made by researchers such as Sukhareva, Kanner, Asperger, and Frankl highlight common linguistic peculiarities that cannot be assimilated to deficits or impairments. These descriptions prompt a deeper examination of autistic communication profiles and explain why communication can be more effective between autistic individuals but often more challenging between autistic and non-autistic individuals. This article proposes future research directions, including the study of the “hundred languages” of autism, exploration of the pragmatic aspects of autistic language use, and investigation of the expressive and phatic functions of language in relation to the specialized interests of autistic individuals. These research avenues should aim to enhance our understanding of autistic communication and contribute to the development of effective educational and professional support strategies that encompass and adapt to the unique communication styles and preferences of autistic individuals.
Gamified mobile learning: An escape room chatbot to enhance medical terminology acquisition
Authors: Wang Yi-Fan , Hsu Mei-Hua , Yue-Feng Wang Max
Published: February 2025

Objective:

The medical terminology escape room game (MTEG) is a chatbot designed for gamified education that aims to transform memorizing complex medical terminology into an enjoyable experience for medical students. By combining elements of ARCS (Attention, Relevance, Confidence and Satisfaction) theory with escape room adventures, the MTEG aims to enhance medical students’ learning outcomes by making the vocabulary memorization process more immersive.

Method:

This study involved 48 first-year nursing students, divided into an experimental group ( n = 24) and a control group ( n = 24). The experimental group utilized the MTEG for self-study, while the control group relied on traditional learning methods. The study assessed learning outcomes through pre-tests and post-tests and used the ARCS Learning Motivation Questionnaire to assess learning motivation.

Results:

Results showed that the experimental group significantly improved learning motivation, with an average ARCS score increased from 3.82 to 4.33 on a 5-point scale. A medical terminology test result also revealed that the experimental group (mean score: 95.83, SD = 5.22) performed better than the control group (mean score: 91.22, SD = 6.21), with a larger effect size (Cohen’s d = 1.52).

Conclusion:

These findings highlight the potential of gamification techniques to enhance medical terminology acquisition and language learning experience. By incorporating gamification elements, MTEG greatly increased the enjoyment of the learning process, encouraged active participation and significantly improved motivation and performance.
Gaming and Performance Metrics in Higher Education: The Consequences of Journal Lists
Authors: Järvinen Margaretha , Mik-Meyer Nanna
Published: February 2025

This article analyses ‘gaming’ in higher education as a response to performance metrics. It shows how scholars react to performance measurement systems in the form of journal lists that define appropriate outlets for their research. Two types of gaming are identified: the adaptation of research methods and topics to align with the perceived ideals of top US journals, and strategic networking with editors and potential reviewers before submitting articles to prestigious journals. We employ scripting theory to analyse performance measurement and its consequences, differentiating between three levels of scripting: cultural scripts, represented by journal lists; interactional scripts, characterised by participants’ redefinitions/enactments of these overarching scripts; and intrapsychic scripts, focusing on participants’ self-scripting and personal attitudes towards gaming in academia. The study is based on qualitative interviews with 173 associate and full professors in the social sciences in Denmark.
Effect of underwater flexibility of a soiled cloth on the detergency of automatic washing machines
Authors: Sasaki Shigeyo , Kameya Takashi , Oya Masaru
Published: February 2025

To determine the conditions of Terg-O-Tometer (a laboratory-scale test washing machine) that match the cleaning performance in real households, this study compares the detergency performances of a vertical washing machine and a Terg-O-Tometer on cotton and chemical fibers. Consistent with previous studies, the detergency of the Terg-O-Tometer matched that of the vertical washing machine at a rotation speed of 100 rpm for cotton cloth. However, when tested on polyester/cotton blend cloth, the matching rotation speed was 40–80 rpm, depending on the dirt on the fibers. These disparate results are attributed to differences in the frictional force between the cloths generated in the vertical washing machine and Terg-O-Tometer, and to differences in the underwater flexibilities of cotton soiled cloth and polyester/cotton blend cloth.
Invisible Participation: Patients with Oncological and Rare Diseases in Russia
Authors: Temina Olga , Zvonareva Olga , Horstman Klasien
Published: February 2025

Scholars have paid much attention to patients’ participation in healthcare, such as engagement in activities developed by healthcare providers and involvement in patient activism. Our study draws attention to a type of patient participation that has attracted less academic interest—patients’ mundane practices of survival. Bringing together insights from political anthropology, science and technology studies, and the sociology of health, we argue that patients’ everyday practices significantly shape care provision, and thus must be understood as participation in healthcare. We coin the term “invisible participation” to highlight that while patients’ everyday practices constitute participation in their own right, this form of participation remains invisible for scientists, policymakers, and patients themselves. Through ethnographically inspired qualitative study of the practices of patients with oncological and rare diseases in Russia, we distinguish three types of practices that constitute invisible participation: navigating healthcare, filling in for unavailable doctors, and providing access to medicines. We discuss the implications of our findings for both democratic and non-democratic contexts.
Activating L1-attrition: A priming experiment
Authors: Glodstaf Walther , Montrul Silvina
Published: February 2025

The Attrition via Acquisition (AvA) model unifies acquisition and attrition by proposing that intake to the inference engine can come from the first language (L1) or the second language (L2). What this model does not specify, however, is the specific psycholinguistic mechanisms that can lead to attrition nor how partial representation may come about. This study expands the AvA model by incorporating activation as a key mechanism and precursor to attrition, and tests the proposal with cross-linguistic priming in bilinguals. We present data from two studies of Finnish and Estonian/English in a community of long-term L1 Finnish emigrants in USA, Canada, Australia, and Estonia. The target condition were the alternation between the marked and unmarked form of the accusative, and marked accusative and partitive, since these two morphemes have been previously documented to suffer attrition in contact with English. Although results did not indicate cross-linguistic priming from either English or Estonian into Finnish, there was evidence of within-language priming in the English–Finnish bilinguals. These findings support the incorporation of activation into the model, but also suggest that the source of attrition for morphology in particular might not come from the L2.