bluuebunny/crossref_metadata_2025 huggingface dataset:
Перевага селекції пар донор – реципієнт за мнс антигенами ii класу hla-dr
Authors: Tashevska N. I. , Nedzvetsky V. S. , Perstneva L. K.
Published: May 2016

The advantage of donor-recipient pairs’ selection HLA for kidneys transplantation by the second grades HLA-DR determined by a method of DNA typing as compared with the selection based on the antigens of the first grade has been studied. It has been found that genetic typing is highly sensitive and specific. The results of the research show that the selection by the antigens HLA-DR is more efficient.
Особливості ситуативної поведінки щурів-агресорів у тесті «перегородка»
Authors: Shlyahova A. V.
Published: May 2016

The peculiarities of behaviour reactions of rats-aggressors to the change of a partner in a «partition» test has been investigated under modelling of sensory contact under the conditions of agonistic collisions. Correlations between indices of the orientation to a partner, and between motor and emotional reactions testify to the increasing interest in the unknown partner with the submissive type of behaviour and to activity and nervous excitement, related to a high level of aggressive motivation.
Роль septum та його взаємодій зі структурами емоціогенного мозку в ініціації та розвитку агресивної поведінки
Authors: Bevzyuk D. A.
Published: May 2016

The role of septum in the mechanisms of aggressive behaviour formation has been studied. It has been established, that forced stimulation of septum leads to diminution of aggressive manifestations in behaviour. Electrical destructions lead to outburst of strong aggression in behaviour. Behaviour is accompanied by a considerable increase of convulsive paroxysmal activity. Monoamines are neurochemical substances ensuring this behaviour. They form complicated hierarchical interrelations between each other.
Вплив промислових електричних знарядь лову на іхтіофауну ізольованих штучних водойм Дніпропетровської області
Authors: Kochet V. M. , Khristov O. O.
Published: May 2016

The influence of the fishing electrical trawl ELU-4M on ichthyofauna in artificial ponds is studied. The comparative analysis of the results obtained and the literature sources is presented. Negative and positive consequences of the electrical trawl application for industrial fishery and regulation of its use are described. It is determined that the exploitation of the industrial electrical trowl (taking into account some ambiguous influence to the biota) in artificial isolated ponds doesn`t influence catastrophically on a hydroecosystem. The work regulation of trowl consists in limitation of its seasonable and diurnal uset, and also in the fishing locations.
Порівняння захисної реакції Rasbora paviei (Cyprinidae) із різних місцеперебувань
Authors: Stolbunov I. A.
Published: May 2016

Experimental investigation of defensive behavior of Rasbora paviei Tirant, 1885 from riverine and limnetic habitats was carried out. Fish for the experiment were caught in the upper stream of Cai river and shallow littoral of Cam Lam reservoir of Khanh Hoa province of Central Vietnam. Billfish Lepisosteus sp. was used as a model predator for the experiment. Distinctions in defensive reaction of R. paviei from different ecological conditions were found. It was revealed that defensive behavior of fish from river’s upper stream was more effective than that of fish from the shallow littoral zone of the reservoir.
Рівень гіалуронату та загальної гіалуронат-зв’язувальної активності білків за умов розвитку експериментального хронічного гепатиту С та лікування альфа-кетоглутаратом
Authors: Fomenko O. Z. , Ushakova G. A. , Pierzynowski S. G.
Published: May 2016

The increase of hyaluronic acid concentration in the blood serum of rats during modelling of chronic hepatitis C is presented. The research of changes in the absolute and relative hyaluronate-binding activity of cytosolic proteins in the rats’ cerebellum and hippocampus under normal condition, experimental chronic hepatitis C and with the alfa-ketoglutarate treatment was carried out.
Біологічні властивості бактерій родини Enterobacteriaceae – збудників гнійно-запальних захворювань у дітей
Authors: Bektascheva O. R. , Lavrentyeva K. V. , Cherevach N. V. , Vinnikov A. I. , Moskalenko А. А.
Published: May 2016

It was investigated the biological properties of 27 strains of Enterobacteriaceae isolated from 24 children with suppurative-septic infection from the Surgical Department of Dnieprodzerzhinsk Hospital № 7. Isolated microflora was presented by four bacteria species: Esсherichia coli, Enterobacter aerogenes, Klebsiella оxytoca and K. pneumoniae. Esсherichia coli was the most widespread agent of suppurative-septic infection for children. All bacteria were the most sensitive to fluoroquinolones ІІ generation (norfloxacin and ciprofloxacin) and cephalosporins ІІІ generation (cefotaxim and cefoperazon).
Деякі аспекти взаємозв’язку концентрації сперматозоїдів в еякулятах із концентрацією Ca2+, K+ та Na+ у тканинах статевих органів і спермі
Authors: Maksymjuk H. V. , Vorobets Z. D.
Published: May 2016

The functional state of tissues that produce spermatozoa and sperm plasma in the bulls reproductive system forms different concentration levels and ratios of Ca2+, K+ and Na+ in the ejaculate. We assumed that due to the work of ion pumps the mismatch of ion concentration in the sperm and genital tissues provides a passive or active transportation of spermatozoa and secretion of seminal plasma from the scrotum to ampulla of ductus deferens.
Вплив антропогенної трансформації території Дунай-Дністровського межиріччя на природну вогнищевість туляремії
Authors: Rusev I. T.
Published: May 2016

The paper is devoted to the problem of occurrence of bacteria Francisella tularensis in steppe coastal zone of the Black Sea west part in the second half of XX century. The key factor decreasing the activity of tularaemia natural foci is anthropogenic influence. Resumed activity of natural foci appeared after implementation of big irrigation and drainage construction in former USSR – building of the Danube-Dniester-Dnepr irrigation system resulted in forming new environmental conditions as well as corridors for tularaemia distribution. The practical recommendation is to implement the eco-epizootological monitoring and to collect data for developing practical management of the natural foci of disease.
Викликані потенціали спинного мозку за дії на нього механічних подразнень
Authors: Shugurov O. O.
Published: May 2016

The electrical evoked potentials (EP) of spinal cord (SC) in reply to the mechanical activation were investigated. The energy of stroke variated from 1·10–3 up to 4·10–3 J. The registration of EP is realized in a L5 segment of SC, the point of stimulation was sequentially moved upwards along the SC. We have shown, that the augmentation of stimulus force produced the increase of amplitude of a potential and its duration. After excluding a place of stimulation from a place of registration the potential was decreased proportional to the square of the distance. We concluded that the mechanical percussions of the SC resulted in the development of long-term oscillating activity of neurones of SC.
Інтегральна оцінка функціонального стану організму дітей і підлітків залежно від біоритмологічного типу працездатності
Authors: Goncharenko M. S. , Timchenko G. M.
Published: May 2016

The paper describes the scientific basis of a complex estimation of functional state of the children's organisms as a whole and their adaptive responses to living conditions (anthropogenic stress, social conditions of training) depending on age and biorhythmic type. The scheme of complex estimation for the children's adaptive capacities, including estimation of functional state and functional parameters, considers age and biorhytmological characteristics. That scheme is presented as a methodological basis of the research. The necessity of determination of children's functional state depending on their age and biorhythmic type to control circadian rhythms of physiological functions is proved. It indicates the stability of the endogenous biological clocks due to interactions of nervous, endocrine, lymphatic, cardiovascular, urogenital and digestive systems.
Селекція високопродуктивного штаму ентомопатогенних бактерій Bacillus thuringiensis, перспективного для захисту природних біоценозів
Authors: Dyrda N. S. , Dregval O. A. , Cherevach N. V. , Vinnikov A. I.
Published: May 2016

The strain variation B-2-6 with a higher production of spores (3.93 × 109 КFU/ml in that variation vs 1.94 × 109 КFU/ml in the initial strain) and endotoxin (10.1 mcg/ml vs 4 mcg/ml respectively) was obtained by means of the ultraviolet irradiation of the В-2 strain Bacillus thuringiensis spores. Insecticidal activity of the obtained variation is characterized by the deaths of 64.3 % of the great brown twist Archips podana at the 3rd day and 97 % at the 10th day after the treatment, which is 20.4 % higher than B-2 strain activity. Possibility of the obtained variation implementation for the natural plants protection against the leaf-eating insects is under discussion.
Нематоди диких копитних України
Authors: Dovgyi Y. , Shendrik L. , Feschenko D. , Boyko O. , Faly L.
Published: May 2016

Strongylata, Rhabditata and Ascaridata eelworms were found in wild hoofs (roe deers and wild boars) in Ukraine. Strongylata are presented by Globocephalus sp., Dictyocaulus viviparous (Bloch), D. еckerti Skrjabin, Muellerius sp., Cystocaulus sp., Protostrongylus sp., Haemonchus contortus Rundolphi, Marshallagia marshalli (Ransom), Nematodirus oiratianus Rajevskaja, Trichostrongylus axei (Cobbold), Bunostomum phlebotomum (Railliet), Oesophagostomum venulosus (Rudolphi), O. dentatm (Rudolphi) and Chabertia ovina (Raill.). The helmints Strongyloides papillosus Wedl, S. ransomi Scwartz et Al. and Ascaris suum (Goeze) were identified for Rhabditata and Ascaridata.
Просторова мінливість електропровідності ґрунту під впливом рийної активності сліпаків на різних масштабних рівнях
Authors: Zhukov A. V. , Konovalova T. M.
Published: May 2016

The soil mounds emerged owing to the mole rats’ digging activity have been shown to be characterised by less electrical conductivity than surrounded soil. This effect is due to the changes of the mounds bulk’s density and moisture. The effect of the mole rats’ digging activity on the soil electrical conductivity has been found not to be restricted by the geometrical border of the mounds. The mounds are surrounded by 1–1.5 m halo of increased soil electrical conductivity. The halo size is increased with the aging of the mound and with the compacting of their aggregation.
Моніторинг підземних вод Полісся
Authors: Zakharkevich I. V. , Zapolskiy A. K.
Published: May 2016

Qualitative composition of the drinking water from underground sources of the decentralized water-supply of the Polissya region with intensive agriculture has been analysed. The sanitary inspection of water sources was conducted. Monitoring of underwaters quality testifies the admissible content of chlorides which are within the normative limits, but fluorides are considerably less than physiological requirements. High level of iron was registered in all studied districts but the nitrate-ion is in Naroditscky and Popylyansky districts. In Naroditscky and Ovrutsky districts the quality of drinking water may be characterized as moderately polluted. Advices on the improvement of the drinking water quality are proposed.
Чутливість мікроміцетів до важких металів
Authors: Korinovskaya O. N. , Gryshko V. N.
Published: May 2016

The sensitivity of 33 micromycete species to nitric compounds of copper, lead, zinc, nickel and cadmium has been determined. Absidia butleri Lendn, Mortierella vanesae Dixon-Stewart, Cunninghamella echinulata Thaxte, Curvularia tuberculata Jain, Cladosporium cladosporiodes (Fresen) G. A. de Vries and Fusarium solani (C. Mart.) Appel et Wollenw are sensitive to minimal content of the heavy metals (0.75 of maximum permissible concentration (MPC)) in the growth medium. At the same time Trixoderma longibrachiatiim Rifai, Alternaria alternatа (Fr.) Keissl and Penicillium sp. 4 demonstrated moderate growth under maximal concentration (50 MPC). It is determined that minimal content of the heavy metals in the initial stage of influence (up to 48 h) promotes growth of only Fusarium oxysporum E. F. Sm. et Swingle, while retards growth of the other species.
Synthetic positive controls for ELISA test kits for detection of IgA and IgM antibodies to Chlamydia trachomatis
Authors: Galkin O. Y. , Gorshunov Y. V. , Besarab O. B.
Published: May 2016

The enzyme-linked immunosorbent assay (ELISA) is the most informative and versatile method of serological diagnostics. The possibility of detecting by ELISA specific antibodies of different classes allow to differentiate primary infectious process and its remission, exacerbation and chronic disease (holding of differential diagnosis). This approach is implemented in the methodology for evaluation of patients for presence of humoral immune response against the causative agent of urogenital chlamydiosis. As with other infections immediately after Chlamydia trachomatis infection the specific IgM antibodies are formed, and subsequently basic projective antibodies of IgG class are synthesized. However, at exacerbation of chronic urogenital chlamydiosis specific IgA antibodies can be synthesized. That is why comprehensive evaluation of patients for presence of humoral immune response to Ch. trachomatis involves plasma testing of specific antibodies of all three classes. The essential problem in the production of ELISA diagnostic kits is obtaining of positive control. The classic version of positive control is human blood plasma containing specific antibodies. But specific IgM- and IgA-positive sera are deficit raw materials. This fact can significantly limit the production of diagnostic kits, especially in case of large-scale manufacture. We have suggested methodological approach to use of synthetic positive controls in indirect ELISA kits based on conjugate of normal human IgM (IgA) and monoclonal antibodies against major outer membrane protein of Ch. trachomatis. It was found that it’s possible to realize such task by means of NHS ester-maleimide-mediated conjugation (by sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) and reductive amination-mediated conjugation (by sodium periodate). It was found that synthetic positive controls obtained by different methods are characterized by higher titer compared to IgM- and IgA-positive high-titer serum. However, positive control obtained by NHS ester-mediated maleimide conjugation has the best titration profile characteristics, at the release time and after one-week storage at37 °C.
Structural trace of adaptation in motive nuclei of spinal cord of rats in hypokinesia and after physical loading in the recovery period
Authors: Popel S. L. , Mitckan B. M. , Krasnopolsky S. Z. , Melnik I. V.
Published: May 2016

The purpose of this paper is to study the morphological changes of neurocytes in spinal cord of rats in hypokinesia and subsequent physical loading. Studies were performed on 55 laboratory rats of Wistar line. Materials of the research were the anterior horns of the gray matter of L5-S2 spinal segments. Preparations stained by Nissl and Viktorov were examined histologically. Hypokinesia was modeled following on the author’s technique. It was established that during prolonged hypokinesia in neurocytes of spinal cord of rats morphological changes in cell size and shape of the motor nuclei of all segments under study have been recorded. The number of hypochromic, hyperchromic destructively unchanged and hyperchromic destructively altered neurocytes increase; shadow cells appears, as well as cases of satellitosis and neuronophagia. Decrease in of albumen synthetical neurocyte function has been recorded. Physical loading of the average aerobic capacity leads to normalization of structural and functional state of neurocytes and enhances the reparative processes, as evidenced by a number of positive changes in morphometric parameters: increase in the number of normochromic neurocytes and decreasing the number of hyper- and hypochromic neurocytes with destructive signs, absence of pyknotic forms. Morphological parameters of neurocytes and their nuclei after physical loading of average aerobic capacity do not differ from those in the control group of intact animals. In neurocytes of this group of rats RNA concentration increases by 12.6% compared to animals after prolonged hypokinesia. Neurocytes of spinal cord of rats after prolonged hypokinesia develop significant morphological changes which are characterized by emergence of a significant number of hyperchromic neurocytes with signs of destructive changes and shadow cells, as well as and hypochromic neurocytes with signs of destructive changes, reduction in size and change of shape of perikaryons of neurocytes and their nuclei. Morphological changes of neurocytes after prolonged hypokinesia are accompanied by violations of biosynthetic processes, as evidenced by RNA decrease in the cytoplasm of efferent neurocytes of spinal cord of rats. Physical loading of average aerobic capacity leads to normalization of structural and functional state of neurocytes and promotes the reparative processes suported by positive changes in morphometric parameters.
Glutathione metabolism system under condition of transplantation of muscle tissue in rats
Authors: Kulibaba O. V. , Kozishkurt S. M. , Duzenko O. O. , Vovchuk I. L. , Petrov S. A.
Published: May 2016

The paper presents the results of determining the amount of reduced glutathione, glutathione reductase and glutathione peroxidase activity at transplantation of muscle tissue taken from rats from the same litter. During the work 2 surgical interventions were carried out: transplantation of muscle tissue taken from the same litter of rats, and operation without replanting. Transplantation was performed on nonlinear white male rats weighing 180–300 g. The animals were taken out of the experiment by passing electric current through the medulla oblongata. For donor transplants males from the same litter were used. In adult donor rats abdominal muscle tissue was extracted and transplanted to homologous tissue of the recipient rat. The same procedure was carried out with femoral muscle tissue. Operation without replanting was used to compare the effects of changes in the number of investigated parameters of the surgical exposure. Tissue not subject to any surgery served as a control. Parameters under study were determined on the first, third and seventh day after transplantation. Glutathione levels in tissues were determined byE. Butler, A. Dyubona, B. Kelly. Determination of glutathione reductase is based on the reduction of NADPH registration. Glutathione peroxidase activity was determined by accumulation of oxidized glutathione. Surgery is known to lead to expression of oxidative stress in the organs operated. Special role in antioxidant protection of the body is given to thiols. Expressed hydrophilic properties provide their high content in water fraction of cells and ability to protect biologically important molecules (enzymes, nucleic acids, hemoglobin) from oxidative damage. Property of thiol compounds is known to inhibit both fermented and not fermented free radical oxidation. For the first time it was found that transplantation of muscle tissue taken from animals from the same litter lead to significant reduction of glutathione in the femoral muscle of the recipient and abdominal muscle tissue of the donor and recipient and to significant increase in glutathione reductase activity in the femoral muscle tissue of the donor and in the abdominal muscle tissue of the donor and recipient.
Effect of transplantation of muscle tissue in rats from the same litter on total number of flavins and FAD
Authors: Kobylnik S. N. , Vovchuk I. L. , Dosenko O. O. , Kozishkurt S. N. , Morosova D. V. , Petrov S. A.
Published: May 2016

Riboflavin is a member of redox enzymes involved in fatty acid oxidation and energy generation. Important role of this vitamin is in reproductive function. Exchange of transformation of riboflavin in animal tissues and cells of microorganisms include reactions that lead to synthesis and subsequent collapse of FMN and FAD. It is involved in enhancing antitumor activity of many anticancer drugs, as well as activation of the immune system to kill tumor cells. Issues of transport of riboflavin and its derivatives in animals have been studied enough. Investigations of changes of the balance of riboflavin and its metabolites in muscular tissues before transplantation in rats from one litter and at operation without replanting were conducted, based on the Udenfriend method of flavin determination. Transplantation in the experiment was carried out on white non-linear male rats weighing 180–300 g. Animals were taken out of the experiment by passing electric current through the medulla. Belly muscular tissue was taken from donor rats of the same litter, and that tissue was sewn to homological muscular tissue of the recipient. The same procedure was carried out with femoral muscular tissue. In the course of operation without replanting the same manipulations have been made except for transplantation stage (for determination of the effect of surgical intervention). Tissue not subject to any surgical intervention served as a control. Parameters of the study were measured on the first, third and seventh days after transplantation. Transplantation of muscular tissue caused no changes in total flavin amount. Content of RF + FMN after transplantation of muscular tissue in rats of the same litter decreased in femoral muscular tissue of the recipient. Transplantation of muscular tissues in rats from the same litter lead to increase in FAD amount in femoral muscular tissue of the donor and recipient on the third day of the experiment. Transplantation of femoral muscular tissue lead to acceleration of FAD synthesis from riboflavin and FMN.
Improvement of the method of obtaining human IgA Fc-fragments
Authors: Galkin O. Y. , Gorshunov Y. V. , Solovjova V. F.
Published: May 2016

To address a number of fundamental and applied problems in immunology, molecular and cellular biology and biotechnology it is necessary to obtain Fc-fragments of immunoglobulins. Fc-fragments may be used for studying of the effector functions of antibodies which are mediated by these areas. They are often used as an immunogen to produce anti-specie (based on so-called secondary antibody) conjugate in the development of serological tests for diagnostics (predominantly such conjugate based on monoclonal antibodies). The work is aimed to develop improved methods of obtaining and allocation of Fc-fragments of human IgA. To achieve this objective, optimization of hydrolysis of IgA with subsequent purification of Fс-fragments have been carried out. Improved method of obtaining Fc-fragments of IgA provides: papain hydrolysis of immunoglobulin in the environment of nitrogen for 4 h, allowing to achieve maximum output of Fc-fragments without their further degradation: isolation and purification of Fc-fragments of human IgA by one-stage gel filtration on sephadex G-100; control of purity of the target product by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate and Ouchterlony immunodiffusion. Enzymatic hydrolysis was carried out at the optimal temperature of papain (37 °C). As the oxygen in the air may have inhibitory effect on enzymatic hydrolysis reaction, the reaction mixture was incubated in the nitrogen atmosphere to prevent inactivation of papain. To reduce the incident degradation of immunoglobulin molecules, papain hydrolysis was carried out without using an enzyme activator (cysteine). Usage of the proposed scheme allows obtaining Fc-fragments of human IgA of high purity. Outcome of Fc-fragments after all stages of purification was about 18% of the initial amount of IgA in the preparation. Molecular weight from Fc-fragments of human IgA was equal to approximately 70 kDa.
Relations between blood supply of brain of students and condition of autonomic nervous system and risk factors
Authors: Korovina L. D. , Zaporozhets T. M.
Published: May 2016

The purpose of our research was to estimate the brain blood supply level by rheoencephalography method in junior students of the Medical academy and to determine the blood supply links with the autonomic regulation state, behavioural and alimentary factors. Rheo-encephalographic study, research of the autonomic nervous system state, heart rate regulation and questioning of 17–29 year-old students have been conducted. Basic hemodynamic indices were normal in all surveyed students. Increase in body weight index enhanced the probability of the brain blood supply deterioration. Adaptation mechanisms tension was accompanied by reduction of the rheographic index. Higher blood filling of the brain vessels corersponded to higher ratio “blood minute volume / due blood minute volume” defined taking into account the system arterial pressure. The quantity of links with indicators of the autonomic nervous system state was limited. Nonlinear dependence of the rheographic index on the Kerdo vegetative index was observed: the rheographic index value was the lowest in students with the autonomic balance by the Kerdo vegetative index; it was the highest in the group with the sympathetic prevalence. Risk factor of blood filling decrease was the reduction in the diet variety when foodstuffs of different groups were included into the diet less than twice a week, or they were excluded from the diet completely. Positive correlation of blood supply was observed more often with frequent consumption of fish, vegetables, and fresh fruits. Increase in the regular alcohol intake experience promoted decrease in brain blood supply and increase in asymmetries of blood supply indicators. The effect of alcohol was essential, despite young age of surveyed students and low level of alcohol consumption. Increase in the experience and intensity of smoking was accompanied by deterioration of brain blood supply indicators. Students with the best blood supply had the better academic progress. Observed multiple correlations of blood supply indicators with behavioural factors allowed to assert that reduction of risk factors (control of body weight, variety and full value of a diet, refusal of smoking, alcohol restriction even below doses which are considered safe) would allow to improve the brain blood supply.
The effect of cardioprotectors on protease-іnhіbіtory potentіal in blood and heart of rats with epinephrine-induced myocardial ischemia
Authors: Paronik V. , Shaul’s’ka O. , Diachenko L. , Shevtsova A.
Published: May 2016

Coronary heart disease is one of the major causes of illness and of deaths in Europe, therefore the definition of the signaling pathways involved in cardioprotection represents a challenging goal in order to discover novel pharmacological approaches. One of the mechanisms included in the implementation of signaling methods is proteolysis, so the aim of our study was to study the balance between activity of the proteolytic enzymes and their inhibitors in the experimental myocardial ischemia of rats and the impact of corvitin (C) and doxycycline (D) on these parameters. Ischemia was induced in Wistar rats by the intraperitoneal injection of epinephrine at a dose of 0.2 mg/100 g of weight during 10 days. All animals were divided into 4 groups: 1 – control (8 rats), 2 – rats with epinephrine-induced myocardial ischemia (EIM, n = 10), 3 – rats, which were entered C after completion of epinephrine injections (n = 10), 4 – rats, which accepted D during 6 days after finishing of epinephrine injections (n = 10). The electrical activity of the heart was recorded on an electrocardiogram (ECG). Activities of matrix metalloproteinases 2 and 9 (MMP2, MMP9) were studied by enzyme-zymography, the activity of trypsin-like enzymes (TLE), levels of alpha-1 proteinase inhibitor (IP1) and alpha-2-macroglobulin (α2-МG) in the blood and the heart were determined by spectrophotometric methods. The experimental results suggest that epinephrine injections lead to changes in the ECG typical for ischemic myocardium. The data obtained showed that the protease-іnhіbіtory balance in the blood and in heart was disturbed. Activity of MMP9, proMMP9/2 and level of IP1 increased in blood plasma of rats with EIM, but activities of ММP2, TLE were unchanged in this group. In the heart muscle the activity of MMP2 and level of IP1 increased. Administration of C resulted in the recovery of MMPs level, but disturbed the balance in the system TLE/IP1-α2-МG. Administration of D blocked the activity of MMP9, significantly reduced the activity of MMP2, which testifies the inhibitory effect of this drug on the activity of matrix metalloproteinases. Based on these results, we conclude that definition of protease-inhibitory balance in the blood plasma of patients with coronary artery disease is promising as an additional diagnostic complex and for monitoring of the effectiveness of therapy.
The effect of citrate and sulfate of different metals on carbohydrates composition of medicinal mushroom Trametes versicolor (Polyporales, Polyporaceae)
Authors: Al-Maali G. A. , Bisko N. A. , Ostapchuk A. M.
Published: May 2016

The aim of our research was to study the influence of citrate and sulfate of copper, manganese and zinc on the carbohydrates composition of biomass of the medicinal mushroom Trametes versicolor (L.) Lloyd. cultivated in a liquid medium. The studied strain of Trametes versicolor 353 was obtained from the Culture Collection of Mushrooms (IBK) from the M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine. Citrates of copper, manganese and zinc were obtained from the Institute of Nanobiotechnologies and Resource Conservation of Ukraine, Kyiv. In this study we used glucose-peptone-yeast extract medium (GPY) with the following composition of (g/L): glucose – 25, peptone – 3, yeast extract – 3, K2HPO4 – 1, KH2PO4 – 1, MgSO4 ∙ 7H2O – 0.25, distilled water – 1,000 ml; pH 6,5 (control medium). Cu2+ (sulfate form or citrate form) were added to control medium in concentration 4 mg/L, Zn2+ and Mn2+ (sulfate form or citrate form) were added to control medium in concentration 1 mg/L. Mycelium was grown in a submerged culture on a rotary shaker (120 rpm) at 26 °C in 250 ml Erlenmeyer flasks, containing 50 ml of liquid medium. The biomass was harvested after 9 days of cultivation in the liquid medium. Monosaccharides and sugar alcohols was analyzed by high-performance liquid chromatography Agilent 1200 (Agilent technologies, USA). The results of our research demonstrated that the fraction of total carbohydrates from T. versicolor 353, which were cultivated on the control medium, consists of glucose (91.5%), galactose (1.9%), mannose (5.4%), fucose (0.6%) and xylose (0.7%). The adding of sulfate of zinc or copper to the liquid medium didn’t change the content of monosaccharides of T. versicolor 353 biomass relative to the control medium. At the same time, in the medium with citrate of zinc the amount of glucose increased and the amount of mannose decreased relative to the control medium. Xylose and fucose were not detected in this case. In mycelium of T. versicolor 353 that was cultivated in the medium with manganese sulfate or manganese citrate we detected a small amount of ribose. But the content of other monosaccharides didn’t change in the medium with sulfate of manganese. At the same time, the addition of citrate of manganese to the medium raised the amount of mannose and reduced the amount of glucose in the biomass of T. versicolor 353 relative to the control medium. Also copper citrate reduced the content of glucose in mycelium of T. versicolor 353 relative to the control medium. But in this case we detected mannitol and sorbitol in the biomass. This data indicates that synthesis of sugur alcohols in T. versicolor 353 depend on copper ions in bioactive forms of citrate.
The role of the supporting lower limb function in the premotor changes in the H-reflex of m. soleus
Authors: Ivanchenko E. Z.
Published: May 2016

In studies on healthy humans we examined changes in the H reflex amplitude and the intensity of background EMG of the soleus muscle within the premotor period of voluntary extension of the contralateral ankle joint. The tested persons were in the lying prone position with relaxed legs, or in the standing position with support on both feet or only on one foot with additional hand support. The soleus H reflex was induced using the standard technique by transcutaneous stimulation (1 ms long current pulses) of the tibial nerve afferents in the region of the popliteal dimple. Using superficial electrodes, we recorded the integral EMG reflex discharge from the m. soleus, amplified, and visualized it using a two-channel digital oscillograph, Handiscope HS3 (TiePieEngeneering, Netherlands). In other tests, we also recorded tonic background EMG activity from the soleus muscle. After fullwave rectification and low-frequency filtration we estimated the area under the integral curve enveloping oscillations of the above mentioned EMG. In the lying position the extension of contralateral ankle joint evoked anticipatory facilitation of the testing soleus H-reflex. It manifested in 90–60 ms and reached the maximum 30 ms before the conditioning movement. Considerable changes of the soleus background EMG-activity did not occur during the premotor period. Consequently, it is supposed that the mechanism leading to the H-reflex facilitation is the decrease of presynaptic inhibition at 1a. In the standing position with support on both feet contralateral ankle joint extension caused anticipatory inhibition of the soleus H-reflex and simultaneous suppression of background EMG 90–60 ms before the conditioning movement. These results indicate the anticipatory changes of muscle tone and suggest participation of postsynaptic mechanism in the inhibition of the testing H-reflex. When the moving contralateral extremity had no support, the contralateral ancle joint extension did not cause anticipatory inhibition but facilitation of the soleus H-reflex. Its time course was similar to that observed in the lying position. The considerable changes of the soleus background EMG-activity did not arise. The results of the experiments indicate that the nature of premotor changes in the spinal neuron circuits, associated with the lower limb muscles, depends not only on the type of upcoming voluntary movement. Being caused by the contralateral limb movements, they depend greatly on the fulfillment or nonfulfillment of the support function by the lower limbs.
Determinación de ácaros del polvo en 90 hogares de tres localidades de Bogotá, Colombia
Authors: Poveda Daniela , Chang Andrés , Cuadros Gustavo , Acero Victor
Published: May 2016

Los ácaros son microorganismos que se encuentran presentes en el polvo común de los hogares y que, en la mayoría de las ocasiones, generan enfermedades de origen respiratorio y dermatológico. Esto afecta significativamente la calidad de vida y hace que esta problemática sea de interés en salud pública veterinaria, debido a la posible relación de los ácaros con la tenencia de animales. El objetivo general de este trabajo fue determinar la cantidad de ácaros intradomiciliarios/gramos de polvo en noventa hogares de tres localidades de Bogotá (Ciudad Bolívar, Rafael Uribe Uribe y San Cristóbal). La metodología se basó en la recolección de muestras por medio de aspirados de colchones, almohadas y piso. Se trabajaron treinta hogares en las tres localidades mencionadas, para un total de noventa familias y 270 muestras de polvo. Los resultados por familia se condensaron en un formato establecido, en el cual se especificó la cantidad de ácaros por gramo de polvo obtenido. Posteriormente, los datos se analizaron con el uso de estadística descriptiva y medidas de tendencia central. Se encontró que la especie más determinante fue el Dermatophagoides pteronissynus con mayor porcentaje en almohadas (Ciudad Bolívar 71,32%, Rafael Uribe Uribe 69,66% y San Cristóbal 75%). Sin embargo, en colchones y piso también se evidenció una predominancia porcentual. Los mayores promedios de ácaros/gramo de polvo se encontraron en colchones de las localidades de Ciudad Bolívar (92,50) y San Cristóbal (617,49), mientras que en la de Rafael Uribe Uribe lo obtuvo el piso (36,59).
Biomarcadores cardiacos como ayuda diagnóstica en las cardiopatías
Authors: Correa Ricardo , Galvis Santiago
Published: May 2016

En la clínica diaria de pequeños animales, es común encontrarse con patologías cardiacas, para cuyo diagnóstico se utilizan técnicas imagenológicas como la radiografía, el electrocardiograma y la ecocardiografía. Sin embargo, a pesar de la amplia investigación sobre estas técnicas, todavía es difícil establecer con claridad el pronóstico de estos pacientes y evaluar objetivamente la evolución de dichas patologías; incluso, existen pacientes con cardiopatías ocultas que pueden pasar inadvertidas al examen clínico y a las ayudas imagenológicas. Es por este motivo que en los últimos años se vienen estudiando nuevos métodos para diagnosticar cardiopatías de forma rápida y eficaz; es el caso de los biomarcadores cardiacos, como las troponinas y los péptidos natriuréticos, que en medicina humana y animal se usan cada vez más para ayudar a establecer el diagnóstico, el pronóstico y la evolución de problemas cardiacos, con alta especificidad y sensibilidad. El presente artículo pretende actualizar los conceptos sobre el manejo de biomarcadores cardiacos en el diagnóstico cardiológico de perros y gatos.
Desarrollo de piómetra y su relación con ovarios poliquísticos en hembras caninas
Authors: Duarte Luz , Sánchez Favio , Ortega Carolina
Published: May 2016

El presente trabajo tuvo como objetivo encontrar una relación entre el desarrollo del complejo hiperplasia endometrial quística (heq) - piómetra y los ovarios poliquísticos, por medio de la observación y las mediciones macroscópicas de úteros de pacientes caninas diagnosticadas con piómetra, a las que se les hizo cirugía de ovariouterotomía para la resolución del problema; dichos órganos fueron obtenidos en dos clínicas de Bucaramanga. Todas las variables registradas fueron tabuladas en Excel 2003 y analizadas mediante el programa Epi Info 7, a fin de hacer un análisis estadístico descriptivo univariable, una tabla de contingencia y una prueba de Fisher. Se correlacionó la presentación de piómetra y de heq con el hallazgo de ovarios poliquísticos (OP). Estadísticamente, no se encontró asociación entre estas variables, (p > 0,05). La existencia de heq fue del 87,10% del total de los casos con respecto a la frecuencia de ovarios poliquísticos, dado que solo se encontró un valor correspondiente al 32,26% sin relación estadística entre estas dos patologías. La literatura reporta que la etiología del complejo heq es por aumento de hormonas estrogénicas en la etapa del diestro [1] y este desequilibrio hormonal, a su vez, es responsable del desarrollo de ovarios poliquísticos; por lo tanto, sería pertinente elaborar un estudio con mediciones hormonales de las pacientes caninas que sean diagnosticadas con piómetra y verificar los hallazgos macroscópicos de los casos.
Estandarización de una PCR anidada para la identificación de Lawsonia intracellularis en porcinos
Authors: Jiménez Ángela , Cortes Luz Stella , Martinez Marcela , Silva Yuliana , Florez Carolina , Mendez Marcel , Gómez Anderson
Published: May 2016

Se estandarizó una técnica de Reacción en Cadena de la Polimerasa PCR anidada para el diagnóstico de la enteropatía proliferativa porcina causada por la bacteria Lawsonia intracellularis. La técnica se basó en la amplificación de una región de 270 pb del gen 16s arnr de Lawsonia intracellularis; para tal fin, se extrajo el adn de la bacteria mediante el kit Purelink Genomic, Invitrogen®, a partir de 100 muestras de íleon de porcinos provenientes del área metropolitana de Bucaramanga (Santander). Los adn fueron cuantificados por fluorometría y ajustados a una concentración de 20 ng/µL. Se llevó a cabo una primera amplificación de la porción de 319 pb del gen 16s arn con los iniciadores externos: 5’-tat ggc tgt caa aca ctc cg-3’ y 5’-tga agg tat tgg tat tct cc-3’. El producto de esta primera amplificación se usó de molde para una segunda reacción, en la que se amplificó una porción interna de 270 pb del gen 16s arn de L. intracellularis, empleando los iniciadores internos específicos: 5’-tta cag gtg aag tta ttg gg-3’ y 5’-ctt tct cat gtc cca taa gc-3’. Las condiciones óptimas para las pcr tanto con los iniciadores internos como con los externos incluyeron: 2,5 mm de mgcl2; 0,15 mm de cada dntp; 1x de Buffer de reacción; 0,8 µm de cada iniciador; y 2,5 U de Taq polimerasa. Como control positivo tanto en la extracción de adn como en las pcr, se empleó ADN bacteriano extraído de la vacuna Enterisol® (Boheringer Ingelheim®). Las temperaturas óptimas de anillamiento para la pcr simple y anidada fueron de 50 y 55°C, respectivamente. El límite de detección de la técnica fue de 5 fg. La técnica no evidenció reacción cruzada con Esquerichia coli, ni con Salmonella typhimurium, bacterias con cuadros clínicos semejantes.
Contribution to the Anatomical Study of Urinary System in Pigs
Authors: Arenas Fausto , Rincón Josimar , Álvarez Juan Camilo , Gómez Fábian Alejandro , Cortés Luz Stella
Published: May 2016

Introducción: son pocos los estudios e investigaciones realizados sobre la caracterización externa e interna del riñón porcino a diferencia de otras especies. El propósito de este estudio fue establecer la incidencia que presenta cada tipo de patrón anatómico de las estructuras vasculares intrarrenales y del sistema de excreción del cerdo, además de su caracterización externa. Materiales y métodos: las variables fueron analizadas en 60 bloques renales provenientes de animales destinados para el consumo humano, lo cuales se obtuvieron éticamente de la planta de beneficio Vijagual de Bucaramanga, Colombia, mediante la inyección de estos con resina poliéster. Resultados: la morfometría externa para ambos riñones presentó una longitud de 120 mm y un ancho de 27,3 mm. Las arterias renales presentaron tres tipos de patrones de bifurcación (patrón I: 96,7%, patrón II: 1,7 % y patrón III : 1,6 %). Un tercer subpatrón (Ic) del grupo I fue encontrado en el 9,4 % del total de muestras. La distribución de las venas tributarias presentó el patrón tipo I (81%) y el patrón II (20%). El patrón III no se evidenció en ninguna muestra examinada. El calibre de los uréteres fue en promedio de 6,9 mm y el ancho de la pelvis renal fue de 12,2 mm. Los patrones de distribución de los cálices se dividieron en dos grupos (A: 30% y B: 70%). Cada patrón presentó una subdivisión en AI , AII y BI , BII. Conclusiones: los patrones de distribución de las estructuras renales encontradas en este estudio son similares a las reportadas en estudios previos, además de la contribución de un patrón arterial (Ic) que se evidenció en esta investigación.
Infección por toxoplasma
Authors: Palmezano Jorge Mario , Plazas Lisette Katherine , Rojas Daniela
Published: May 2016

Tema y alcance: este artículo pretende ofrecer al lector una mirada general sobre la toxoplasmosis, una zoonosis causada por Toxoplasma gondii, que afecta tanto a los hospederos intermediarios, como a los definitivos; buscamos que pueda entenderse la dinámica de la enfermedad, dejando de lado ciertos mitos sobre esta.

Características: los félidos (Felidae) son los hospederos definitivos, entre los cuales encontramos a los gatos como los más cercanos al ser humano, que desarrollan la forma sexuada del parásito, arrojando al medio los ooquistes infectivos; de allí que su presencia es esencial en el ciclo biológico de T. gondii.

Hallazgos: las investigaciones sobre gatos a nivel mundial son limitadas ya que existe una alta complejidad en la toma de muestra; sin embargo, se dispone de un amplia variedad de estudios sobre la taxonomía, morfología, ciclo de vida y biología molecular, así como sobre la distribución epidemiológica, sus hospederos, las vías de transmisión, las fuentes de contaminación y los factores de riesgo asociados con la infección dentro de esta especie y hacia otras vulnerables.

Conclusiones: el conocimiento acerca de esta parasitosis es de gran importancia, sobre todo en los casos de mujeres embarazadas y personas inmunodeprimidas, en quienes se puede manifestar complicaciones de la enfermedad.
Osteopatía hipertrófica secundaria a metástasis pulmonar de carcinoma mamario
Authors: Correa Ricardo Andrés , Giraldo Juan Carlos
Published: May 2016

Propósitos: este artículo pretende reportar los hallazgos de un caso clínico de osteopatía hipertrófica.

Tema: la osteopatía hipertrófica es un raro desorden paraneoplásico, asociado con el sobrecrecimiento doloroso del periostio de los huesos largos, normalmente desencadenado por neoplasias primarias o metastásicas de pulmón.

Desarrollo: se presenta el caso de un rottweiler, de 12 años, con osteopatía hipertrófica asociada con metástasis pulmonar de un carcinoma mamario.

Conclusiones: se comentan los hallazgos clínicos, radiográficos y las posibilidades terapéuticas.
Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados
Authors: Valleriote Patrícia , Paes de Carvalho Carla , Dubeibe Diego Fernando , Mogolllón Edgar Mauricio , Burla Angelo Jose
Published: May 2016

Introducción: na espécie bovina, o oviduto joga um papel fundamental nos processos de maturação, fertilização e aquisição de competências para o adequado desenvolvimento do embrião até blastocisto. Visto que as células do revestimento interno do oviduto proporcionam substancias que afetam positivamente o desempenho dos espermatozoides, à medida que os espermatozóides atravessam o trato genital da fêmea, os componentes da superfície celular são modificados ou removidos pelas secreções ali presentes, ditas modificações podem incluir depleção do colesterol na superfície espermática, alteração nos glicosaminoglicanos e mudanças nos íons.

Metodología: com base nestas razões objetivo-se neste trabalho avaliar a influência da adição de meio de cultura de células de oviduto bovino sobre parâmetros espermáticos como motilidade, vigor e integridade de membrana citoplasmática. Os espermatozoides foram incubados com 164 μL de Talp-sp-Uso no grupo controle (TCt); 161,5 μL de Talp-sp-Uso, acrescido de 2,5 μL de heparina (20µg/mL) no tratamento 1 (THep) e 128 μL de Talp-sp uso, acrescido de 36 μL de meio de cultivo com secreções de células do oviduto bovino no Tratamento 2 (TOv). As avaliações foram feitas no tempo zero (para mostrar que os meios dos diferentes tratamentos não prejudicaram os espermatozoides) e após 3 horas de incubação.

Resultados: não foram encontradas diferenças significativas nos parâmetros de motilidade progressiva, vigor e integridade de membrana celular entre os tratamentos propostos. No entanto, espermatozoides cultivados com secreções de células do oviduto apresentaram maior percentagem de motilidade total às 3 h de cultivo, em comparação a espermatozoides cultivados na presença de heparina.

Conclusão: as secreções de células do oviduto não afetam a motilidade progressiva, nem o vigor ou a integridade da membrana citoplasmática dos espermatozoides, porém, prolongam a motilidade total dessas células.