bluuebunny/crossref_metadata_2025 huggingface dataset:
Building Intelligent Natural Interfaces for Distributed SCADA Systems Using a Semantic Parsing Framework
Authors: Liang Guicai , Li Yurong , Li Shijun
Published: February 2023

Converting natural language into machine language that can be recognized by distributed systems is the core challenge of intelligent interactive interfaces and human-machine dialogue systems. The human-machine interface interaction of large distributed SCADA measurement and control system is tedious and the operation and maintenance cost is high, so it is significant to design an intelligent natural language interaction interface for distributed measurement and control system. In this paper, we design the intermediate language format of SCADA system, i.e., Key-value logic form, and then formulate the Text2SCADA framework and propose the TICS algorithm and SDPA algorithm, the former adopts the keyword extraction and cosine similarity optimization algorithm to complete the structured extraction of natural language for basic natural language instructions, and the latter adopts the keyword extraction and cosine similarity optimization algorithm to complete the structured extraction of natural language for relatively The latter one adopts the algorithm of dependent syntactic analysis for the structured representation of natural language instructions with relatively complex natural language instructions. Based on the above algorithms, a lightweight information extraction model based on DGCNN and probabilistic graph ideas is constructed, aiming to enhance the scientific generalization ability of the framework on unknown instruction sets. The experimental results show that the proposed intelligent natural language interface can better solve the human-machine interface interaction problem of distributed SCADA measurement and control system. The average accuracy, recall and F-value of instruction parsing reach 89.27\%, 89.28\% and 89.27\%, respectively. The average response time is 1.593 s. Especially, it provides a more convenient means of interaction for industrial and agricultural information control.
Constructions of Mixed Reverse-Order Laws for the Products of Matrices Involving Moore–Penrose Inverses and Group Inverses
Authors: Tian Yongge
Published: February 2023

It is a classic topic in algebras to construct and verify equalities that are composed of various algebraic operations of elements and their inverses or generalized inverses. In this note, the author constructs a matrix equality $(AB)^{\dag} = B^{\ast}(A^{\ast}ABB^{\ast})^{\#}A^{\ast}$ (called a mixed reverse-order law), where $A$ and $B$ are two matrices of appropriate sizes, $(\cdot)^{\ast}$, $(\cdot)^{\dag}$, and $(\cdot)^{\#}$ denote the conjugate transpose, the Moore--Penrose inverse, and the group inverse of a matrix, respectively, and shows that the mixed reverse-order law always holds. A list of variation forms of this matrix equality are also given, and necessary and sufficient conditions are obtained for them to hold. Especially, we show a remarkable fact that the two reverse-order laws $(AB)^{\dag} = B^{\dag}A^{\dag}$ and $(A^{\ast}ABB^{\ast})^{\#} = (BB^{\ast})^{\#}(A^{\ast}A)^{\#}$ are equivalent.
Agriculture Land Cover Identification Using One-Shot Airborne Hyperspectral Images: case study of small parcels, Poland
Authors: Hejmanowska Beata Joanna , Kramarczyk Piotr Szymon
Published: February 2023

This study aimed to investigate the possibility of using one-shot hyperspectral airborne images to recognize crops for an area with many small plots. The results showed that unsupervised clustering methods could classify crops with an accuracy of 80%, which improved to 90% when restricted to only grain crops, using a single airborne hyperspectral recording. However, additional layers such as NDVI, DTM, slope, and aspect did not improve classification accuracy. For comparison, the accuracy of clustering time series Sentinel-2 images with NDVI layers and DTM-derived data yielded an accuracy of: 74% ,Sentinel-2 time series 68% and single one registration before harvest - 39%. The results of the random forest classification were slightly less accurate due to a lack of sufficient reference data. However, it is challenging to verify the reported accuracy of crop recognition in the literature above 90% due to differences in analysis methodologies, reference data selection, pixel/object approaches, metric choice, and calculation formulas used.
Moderating Effect of Business Environmental Dynamism In In-Novativeness-Firm Performance Relationship of Congolese Manufacturing Companies
Authors: Ruba Remo Metalor , Chiloane-Phetla Germinah E. , VAN DER WESTHUIZEN THEA
Published: February 2023

Research has shown that innovativeness is a key factor leading to company performance. However, the business environment can play a detrimental effect on company performance by undermining the company’s internal strategic efforts in terms of innovativeness. To test this, 344 questionnaires were sent to manufacturing companies owners and managers. 178 of them were returned and usable to test the study hypotheses. Though a hierarchical regression analysis, it was found that innovativeness has positive and significant effect on company performance, but business environmental dynamism has a negative and significant effect in innovativeness-company performance relationship. Recommendations and other research perspectives are suggested
Vibrational and Acoustical Wave Propagation Through Fibreglass Plate for Different Boundary Conditions
Authors: Aygun Haydar
Published: February 2023

Fibreglass composite plates are used for applications ranging from aerospace and automotive industries to construction industry. Vibroacoustic properties of the fibreglass plate has been investigated for different boundary conditions. The theory of the plate vibration and sound radiation has been used to determine the deflection of the composite plate at different locations on its surface. 3-Dimentional (3D) deformations and their corresponding contour velocities of plate have been computed for a range of frequencies. It has been shown that clamping the plate at four edges delayed first resonance of the plate by 15 Hz and second resonance of the plate by 30 Hz. Vibroacoustic indicators and the radiation impedance matrix of the plate have also been calculated. The results of this study show that fibreglass plate could be used in building industry to dampen the vibration of machine and structural vibration of buildings, and to sandwich building structures to form panels in order to attenuate airborne sound and to lower noise transmission of structural borne sound
Prevalence of Lumbosacral Transitional Vertebral Anomalies Among Healthy Volunteers and Patients with Hip Pathology
Authors: Verhaegen Jeroen C.F. , Alves Batista Nuno , Horton Isabel , Rakhra Kawan , Beaulé Paul E. , Michielsen Jozef , Stratton Alexandra , Grammatopoulos George
Published: February 2023

Background:

A lumbosacral transitional vertebra (LSTV) has been reported to be prevalent among patients with hip dysplasia. The aims of this study were to determine the (1) prevalence of an LSTV in young patients presenting with hip pain and a group of asymptomatic volunteers, (2) effect of an LSTV on spinopelvic characteristics, and (3) presence of low back pain among patients with an LSTV.

Methods:

This cross-sectional study included 102 patients with hip pathology and 51 asymptomatic volunteers (mean age, 33.9 ± 7.3 years; mean body mass index, 26.0 ± 5.0 kg/m2; 57.5% female). Participants underwent radiographic assessment of the lumbar spine and pelvis in standing and deep-seated positions. LSTV occurrence was classified according to the Castellvi system. Spinopelvic characteristics included lumbar lordosis (including segmental lumbar angles), pelvic tilt, and hip flexion (pelvic-femoral angle). Differences between standing and deep-seated values were calculated. Low back pain was assessed using the Oswestry Disability Index.

Results:

The prevalence of LSTV type ≥II was 8.5%, with no difference between patients and volunteers (p = 0.386). Individuals with an LSTV had a greater standing L1-L5 angle (mean, 51.6° ± 11.7° versus 38.9° ± 9.3°; p < 0.001). The overall spinal flexion (change in L1-S1 angle between the standing and deep-seated positions) in individuals with an LSTV was similar to that in individuals without an LSTV; restricted L5-S1 mobility was compensated for at L1-L2 (10.2° ± 5.8° in those with versus 8.4° ± 4.1° in those without an LSTV; p = 0.070). No significant difference in the presence of low back pain was found (p = 0.250).

Conclusions:

An LSTV was found in 8.5% of young adults, with no difference between patients with hip pathology and controls. Individuals with an LSTV have greater standing lumbar lordosis, with altered mechanics at the cephalad adjacent level, which may predispose these individuals to degenerative changes at this level.

Level of Evidence:

Prognostic Level II. See Instructions for Authors for a complete description of levels of evidence.
The cancer testis antigen TDRD1 regulates prostate cancer proliferation by associating with snRNP biogenesis machinery
Authors: Feng Qin , Kim Hong , Barua Amrita , Huang Luping , Bolaji Modupeola , Zachariah Sharon , Jung Sung Yun , He Bin , Zhou Tianyi , Mitra Aroshi
Published: February 2023

Prostate cancer is the most commonly diagnosed noncutaneous cancer in American men. TDRD1, a germ cell-specific gene, is erroneously expressed in more than half of prostate tumors, but its role in prostate cancer development remains elusive. In this study, we identified a PRMT5-TDRD1 signaling axis that regulates the proliferation of prostate cancer cells. PRMT5 is a protein arginine methyltransferase essential for small nuclear ribonucleoprotein (snRNP) biogenesis. Methylation of Sm proteins by PRMT5 is a critical initiation step for assembling snRNPs in the cytoplasm, and the final snRNP assembly takes place in Cajal bodies in the nucleus. By mass spectrum analysis, we found that TDRD1 interacts with multiple subunits of the snRNP biogenesis machinery. In the cytoplasm, TDRD1 interacts with methylated Sm proteins in a PRMT5-dependent manner. In the nucleus, TDRD1 interacts with Coilin, the scaffold protein of Cajal bodies. Ablation of TDRD1 in prostate cancer cells disrupted the integrity of Cajal bodies, affected the snRNP biogenesis, and reduced cell proliferation. Taken together, this study represents the first characterization of TDRD1 functions in prostate cancer development and suggests TDRD1 as a potential therapeutic target for prostate cancer treatment.
Multiple Machine Learning Methods for Runoff Prediction: Contrast and Improvement
Authors: Chen Yuechao , Zhang Yue , fan xiaolei , Song Xue , Gao Jiajia , Bin Zhaohui , Ma Hao
Published: February 2023

Machine learning methods provide new alternative methods and ideas for runoff prediction. In order to improve the application of machine learning methods in the field of runoff prediction, we selected five rivers with different conditions from north to south in Japan as the research objects, and compared the six watersheds and different types methods of time series prediction in machine learning methods, to evaluate the accuracy and applicability of these machine learning methods for daily runoff prediction in different watersheds, and improve the commonality problem found in the prediction process. The results show that before the improvement, the prediction results of the six methods in Kushiro river, Yodogawa river and Shinano Gawa river are good. After the improvement, the runoff prediction errors of the six methods in the five watersheds are greatly reduced, and the prediction accuracy and applicability are greatly improved. Among them, the improved deep temporal convolutional network (DeepTCN) has the best prediction effect and applicability. Of all prediction results in the five watersheds, the NSE coefficients are above 0.94. In general, the improved DeepTCN has the best comprehensive prediction effect, and has the potential to be widely recommended for runoff prediction
Prevalence of hepatitis B virus and associated factors among pregnant women in the health facilities, N'djamena, Chad
Authors: Debsikreo Nalda , Mankréo Birwé Léon , Ouangkake Merwa , Jotham Madjikoula , Ndiaye Anna Julienne Selbé , Leye Nafissatou , LO Gora , Moukenet Azoukalné , Moussa Ali Mahamat , Toure-Kane Ndèye Coumba , Lunel-Fabiani Françoise
Published: February 2023

Background
Hepatitis B virus (HBV) infection is a major global health problem. In Chad, the prevalence is estimated at 19% in the overall population. Control hepatitis B among pregnant women may help to break the chain of transmission. However, the prevalence and risk factors of hepatitis B among pregnant women in N'Djamena are not documented. The aim of this study was to establish the HBV prevalence and identify the risk factors associated with hepatitis B in pregnant women in N'Djamena.
Methods
We conducted a cross-sectional study in eight health facilities in the city of N'Djamena (Chad) from April 4 to August 2, 2021. HBV surface antigen was determined using the SD Bioline HBsAg WB rapid test. We performed a Chi-squared test and an adjusted logistic regression to identify risk factors associated with hepatitis B infection.
Results
A total of 458 pregnant women were included in the study. The average age of participants was 25 years (95% CI: 20–30). Among risk factors, being older (age > 35 years) and having been tested for HBsAg increase the likelihood of being HBsAg+ (OR = 1.22, 95% CI: 0.33–3.92, p = 0.001, and OR = 4.93, 95% CI: 2.05-12.0, p < 0.001).). Pregnant women whose mothers were AgHBs + were also more likely to be HBsAg+ (OR = 27.8; 95% CI: 4.17–192; P = 0.004).
Conclusion
The prevalence of hepatitis B shows intermediate endemicity in pregnant women in Chad. Age, HBsAg carrier mother, and history of HBsAg screening are associated with HBV infection.). Thus, to avoid mother to child transmission of HBV, here is a need to improve the health education of pregnant women and the access to routine prenatal screening and vaccination of newborns at birth. It is also important to increase the immunization coverage of the population, through children vaccination campaigns.
Preliminary study on the detection of hydrogen ion flux in breast cancer tissue using noninvasive microtest technology
Authors: Lu Xu , Yang Xin , Xu Yue , Zhang Zengkai , Ye Bin , Hua Bin , Porterfield D. Marshall , Li yao
Published: February 2023

Objective
To detect the extracellular hydrogen ion (H+) flux of breast cancer tissue explants using noninvasive microtest technology (NMT) and assess how this flux correlates with the molecular subtype. Further more, we preliminarily explored the possibility of applying NMT as a treatment prescreening tool for predicting how docetaxel will affect tissue responses.
Methods
This study enrolled 30 breast cancer patients who underwent surgery in the Department of Breast Surgery at Beijing Hospital, Beijing, China. Tumor samples and the corresponding normal samples were excised from surgical specimens with a size of approximately 0.5 cm3. The NMT system was used to detect the H+ flux of tumor samples and corresponding normal samples. Then, the NMT system was used to assess the changes in H + flux after the tumor samples were treated with 10 mg/L docetaxel.
Results
All the samples produced stable H + flux that could be measured in real time. In 26 cases, we found that tumor samples exhibited stable and robust efflux of H+, whereas in the corresponding normal samples, we measured significant differences with H+ influx or mild efflux (cancer samples: 0.336 ± 0.307 pmol/cm− 2/sec− 1 vs. normal samples: -0.067 ± 0.131, p < 0.001). Among the 26 tumor samples that showed efflux of H+, there were 4 luminal A type, 11 luminal B type, 6 HER2-positive type and 5 triple-negative type. Triple-negative tumors had a higher rate of H + efflux compared with luminal A, luminal B and HER2 positive tumors (p = 0.016, p = 0.018, p = 0.008). Among the 4 tumor samples that were treated with docetaxel, 3 samples showed inhibition of H + efflux by 50.5%, 28.8% and 8.3%, while efflux increased by 30.0% in 1 sample.
Conclusion
NMT can detect the H + flux of breast cancer tissue in real time. The H + flux of tumor samples was significantly different from that of normal samples. After treatment with docetaxel, the H + flux of tumor samples showed definite changes.
Clothing Online Design and Sales Platform Based on AI Learning and Dynamic Image Recognition
Authors: Hu Yan , Sun Lei , Zhuang Lifeng
Published: February 2023

In the current information technology, the basic feature of dynamic image recognition is to digitize the biological information of different individuals. Compare the features that can be identified, and then realize the recognition function. This article first investigates the current status of the current clothing sales platform management method, introduces the main situation of AI learning, analyzes the work flow of the sales platform, and studies the current needs, and then focuses on whether this technology can improve the platform The work efficiency of the company launched an in-depth discussion. Secondly, it analyzes some problems that may exist in clothing sales management, and then points out a new recognition algorithm based on these analysis results. This algorithm is a combination of dynamic image analysis and deep neural network, which can be used for clothing such as factories. The video provided by the unit conducts dynamic analysis, automatically recognizes and organizes clothing online design and sales, realizes efficient and intelligent clothing transaction statistics, and this makes the whole system run much more efficiently. The design of the system is combined with various problems existing in the process of clothing marketing, and various applications of the system in clothing design and marketing are discussed, so as to give full play to the advantages of dynamic image recognition technology and AI intelligence technology. At the same time, the implementation plan is also expressed, and part of the interface is explained. Finally, this article describes the test situation of the system, mainly including the function test and performance test of the project.
Clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis by Taq polymerase involves nick-translation
Authors: Ohtsubo Yoshiyuki , Kawahara Syoutaro , Nagata Yuji
Published: February 2023

Development and characterization of a new enzyme reaction benefit modern biotechnologies. Here, we report a novel CIS (clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis) reaction catalyzed by Taq polymerase. In the reaction, a single-stranded DNA (ssDNA) with 3′ Cs is attached with a preformed 3′ G-tail of double-stranded DNA (dsDNA); DNA syntheses starting from both 3′ ends result in the incorporation of ssDNA. A 3′ G-tail length of 3 nucleotides adequately supports this reaction, indicating that Taq polymerase can clump short Watson-Crick base pairs as short as three pairs and use them to initiate DNA polymerization. The reverse transcriptase from Molony murine leukemia virus catalyzes strand displacement synthesis and produces flapped-end DNA, whereas the reaction by Taq polymerase involves the nick translation. These new reaction properties may be beneficial for the development of new molecular tools applicable in various fields. Apart from its CIS reaction activity, we also report that Taq polymerase has the undesirable characteristic of removing 5' fluorescent labels from dsDNA. This characteristic may have compromised various experiments involving the preparation of fluorescently-labeled dsDNA by PCR for a long time.
An analysis of wind characteristics of Kolkata airport and prediction of wind speed and direction using a new machine learning technique
Authors: Naskar Pravat Rabi , Naskar Somnath
Published: February 2023

In this study, we have attempted to determine the wind characteristics of Netaji Subhas Chandra Bose International Airport (NSCBIA) and predict wind speed and direction 9, 30 hours ahead using a new machine learning (ML) technique. For this, we have collected METAR wind speed and direction data from the NSCBIA for the period 2016-2021. On analysis of the wind speed and direction data, it is observed that a substantial amount of calm wind exists in NSCBIA. The wind speed varies throughout the day with a maximum around 09-11 UTC. The maximum randomness in wind direction is observed around the 06-09 UTC. The maximum wind speed is observed during April-July. Minimum wind speed is observed in December and January. Generally, wind speed remains low during 23-00 UTC but for winter months (October-January) low wind speed is observed during 15-21 UTC. MAE, RMSE and SSE are low for SSA-LSTM in predicting the wind speed. MAE, RMSE, and SSE are higher in predicting wind direction but within tolerance limits owing to wind direction randomness.
Pleiotropic and independent genetic loci underlie resistance to grain shattering in standing crop of modern wheat
Authors: Emebiri Livinus , Hildebrand Shane
Published: February 2023

Grain shattering in standing crops of wheat (Triticum aestivum L.) is estimated to account for up to 17% loss of harvestable yield. In this study, we analysed data from two bi-parent populations and a wheat diversity panel to explore the underlying genetic basis for grain shattering observed in multiple field experiments through quantitative trait loci (QTL) analysis. Grain shattering had a significant and negative association with grain yield, irrespective of populations and environments. The correlation with plant height was positive in all populations, but correlations with phenology was population-specific, being negative in the diversity panel and the Drysdale × Waagan population, and positive in the Crusader × RT812 population. In the wheat diversity panel, allelic variations at well-known major genes (Rht-B1, Rht-D1 and Ppd-D1) showed minimal association with grain shattering. Instead, the genome-wide analysis identified a single locus on chromosome 2DS, which explained 50% of the phenotypic variation, and mapping to ~10 Mb from Tenacious glume, Tg gene. In the Drysdale × Waagan cross, however, the reduced height (Rht) genes showed major effects on grain shattering. At the Rht-B1 locus, the Rht-B1b allele was associated with 10.4 cm shorter plant height, and 18% decreased grain shattering, whereas Rht-D1b reduced plant height by 11.4 cm and reduced grain shattering by 20%. Ten QTL were detected in the Crusader × RT812, including a major locus detected on the long arm of chromosome 5A. All the QTL identified in this population were non-pleiotropic, as they were still significant even after removing the influence of plant height. These results indicated a complex genetic system for grain shattering in modern wheat, which varied with genetic background, involved pleiotropic as well as independent gene actions, and which might be different from shattering in wild wheat species caused by major domestication genes.
IoT Based Compact, Low-Cost Dyeing Machine for Medium Scale Industrial Environment Applications
Authors: L Dhivya Priya E , M Suresh Chinnathampy , Kavitha K , Sharmila A
Published: February 2023

Colouring clothes use the universal solvent “water” whose Potential of Hydrogen (pH) level is increased when combined with the chemical colouring agents. The proposed design is an innovation that uses centre sprinkler rod which supports to spread of the colour evenly all over the cloth. Arduino board helps in combining water and provisional colour as per the programmed mechanism. Ultrasonic sensor measures the range to which mixed colour must be filled in the container. Arduino board interconnected with ultrasonic sensor helps in operating the sprinkler rod, which supports for the even spread of dye all over the cloth. Even spread of dyes, lever mechanism is used to dip the cloth inside the container with the dyeing solution. The process will be repeated further for a pre-defined duration of time, based on the material of the cloth. After the process, the dyeing solution in the container is allowed to discharge. The pH sensor placed at the outlet will test the pH and will lead to a zero discharge water container. These zero dis-charge water containers incorporate the filtering process where the pH level of the water is reduced. This designed embedded device helps the lower middle-class people economically. The idea of protecting people is the first criterion met up with the designed product. Thus, this idea helps people to run a smooth lifestyle both in terms of money and health. Also, this designed device helps in retaining and reusing water for various other applications.
Staged Open Cranial Surgery for Primary Intra-axial Neoplasms: A Systematic Review
Authors: Bui Nick , Brown Nolan , Gendreau Julian , Patel Neal , Shahrestani Shane , Patel Saarang , Beyer Ryan , Koester Stefan , Singh Rohin , Abraham Mickey , Mammis Antonios
Published: February 2023

Background
Staged surgery for skull base lesions has been utilized to facilitate maximal safe resection and optimize outcomes while minimizing morbidity and complications. Conversely, staged surgery for primary intraparenchymal neoplasms is less commonly performed and has not been reported as extensively within the literature. As such, we performed a systematic review to examine the unique surgical indications for staging, timing between stages, specific surgical approaches employed, and postoperative complications of staged surgery for primary intra-axial neoplasms.
Methods
A literature search was conducted in August 2021 using PubMed, Web of Science, and Cochrane databases using the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) recommendations. Titles and abstracts were evaluated independently by two authors, after which articles were selected for final analysis based on application of strict inclusion criteria during full text screen. Each included article was then qualitatively assessed and relevant variables – including operative approaches, timing, and outcomes – were extracted for synthesis.
Results
Of 115 results, 7 articles were included for final analysis and consisted of 17 pediatric and 4 adult patients. Staged approaches were more commonly utilized in the pediatric patient population for resection of astrocytoma and glioma. Pediatric patients had a timing of surgeries ranging from 5–10 days between operations, compared to 18 days-4 months in adult patients. Complications in pediatric patients were most commonly hemiparesis, hydrocephalus, CN VI and VII palsies, truncal ataxia, and cerebellar mutism, while complications in adult patients included language and abstract thinking deficits, respiratory failure, and motor weakness.
Conclusion
This study reports the first comprehensive review of staged surgical procedures for primary, intra-axial cranial neoplasms. There exists a large degree of heterogeneity in complications resulting from staged surgeries for intra-axial neoplasms, which are similar to complications associated with single-stage surgery for intraparenchymal lesions as well as multi-stage surgeries for skull base lesions.
EDUVI: An Educational-Based Visual Question Answering and Image Captioning System for Enhancing the Knowledge of Primary Level Students
Authors: Gupta Manisha , Asthana Priya , Singh Preetvanti
Published: February 2023

Within the last several years, the revolution in online education has fundamentally transformed the idea of traditional education. The trend of online education is exploding in popularity in the modern educational system. However, it is challenging for primary-level students to adapt to learning in this novel environment. When enrolled in online courses, students at this level encounter a variety of difficulties. Hence, to provide better learning methods, this research study focuses on developing an education based EDUVI system for primary level students which helps the students in visual learning. The proposed system will help the students for self-learning without any assistance using a simplified and interactive platform. The developed system integrates visual question answering and image captioning system where students can import the image and extract answer based on the query asked by them or can generate the description or caption.
Agomir miRNA-150-5p alleviates pristane-induced lupus by suppressing myeloid dendritic cells activation and inflammation via TREM-1 axis
Authors: Yue Chenran , Gao Sheng , Ye Jianzhong , Zhang Ting , Xing Zhouhang , Xie Yuanyuan , Qian Hengrong , Zhou Xueyin , Li Shuting , Yu Anni , Wang Liangxing , Hua Chunyan
Published: February 2023

Triggering receptors expressed on myeloid cell-1 (TREM-1) has been shown to participate in inflammatory autoimmune diseases. Nevertheless, the detailed underlying mechanisms and therapeutic benefits remain elusive, especially in myeloid dendritic cells (mDCs) and systemic lupus erythematosus (SLE). In this study, we screen TREM-1 as one of the hub genes closely correlated with the progression of SLE and identify soluble TREM-1 (sTREM-1) in serum as a valuable diagnostic biomarker for SLE. Moreover, activation of TREM-1 by its agonist promotes activation and chemotaxis of mDCs and increases production of inflammatory cytokines and chemokines, showing higher expression of IL-6, TNF-α and MCP-1. Disorders of epigenetic processes including non-coding RNA give rise to SLE, resulting in complicated syndromes. Herein, we show that lupus mice display a unique miRNA signature in spleen, among which miR-150 is the most significantly expressed miRNA that targeting TREM-1 compared with wild type group. Transfection of miRNA-150-5p mimics directly suppresses the expression of TREM-1 by binding to its 3' UTR. Our in vivo experiments first indicates that administration of miR-150-5p agomir effectively ameliorates lupus symptoms. Intriguingly, miR-150 inhibits the over activation of mDCs through TREM-1 signal pathway in lymphatic organs and renal tissues. Overall, TREM-1 represents a potentially novel therapeutic target and we identify miR-150-5p as one of the mechanisms to alleviate lupus disease, which is attributable for inhibiting mDCs activation through TREM-1 signaling pathway.
Synthesis, antileishmanial, antimalarial evaluation and molecular docking study of some hydrazine-coupled pyrazole derivatives
Authors: Berhe Halefom Gebreselasse , Birhan Yihenew Simegniew , Beshay Botros Youssef , Habib Huda Jawad , Hymete Ariaya , Bekhit Adnan Ahmed
Published: February 2023

Pyrazole-bearing compounds are known for their diverse pharmacological effects including potent antileishmanial and antimalarial activities. Herein, some hydrazine-coupled pyrazole derivatives were successfully synthesized and their structures were verified by employing elemental microanalysis, FTIR, and 1H NMR techniques. The in vitro antileishmanial and in vivo antimalarial activities of the synthesized pyrazole derivatives (9–15) were assessed using the clinical isolate of the Leishmania aethiopica strain and mice infected with Plasmodium berghei ANKA strain, respectively. The result revealed that compound 13 displayed superior antipromastigote activity (IC50 = 0.018) that was 174- and 2.6-fold more potent than the standard drugs miltefosine (IC50 = 3.130) and amphotericin B deoxycholate (IC50 = 0.047). The molecular docking study conducted on Lm-PTR1 justified the better antileishmanial activity of compound 13. Furthermore, the target compounds 14 and 15 elicited better inhibition effects against Plasmodium berghei with 70.2% and 90.4% suppression, respectively. In conclusion, the hydrazine-coupled pyrazole derivatives may be considered potential pharmacophores for the synthesis of safe and effective antileishmanial and antimalarial agents.
Da Vinci robot versus thoracoscopic surgery for lymph node dissection in radical lung cancer: a propensity score matching study
Authors: Hong Ziqiang , Sheng Yannan , Bai Xiangdou , Cui Baiqiang , Wang Kai , Wu Xusheng , Cheng Tao , Jin Dacheng , Gou Yunjiu
Published: February 2023

Objective: To compare the efficacy of robot-assisted thoracoscopic surgery (RATS) with video-assisted thoracoscopic surgery (VATS) in lymph node dissection for radical lung cancer.
Methods: Retrospective analysis of 262 minimally invasive surgically treated lung cancer patients who underwent radical lung cancer surgery via RATS (n=122) or VATS (n=140) completed by the same operator in the Department of Thoracic Surgery, Gansu Provincial People’s Hospital from August 2019 to August 2022. Propensity score matching (PSM) analysis was performed to compare the procedure-related information of the two groups of patients.
Results: All patients in both groups successfully completed radical lung cancer surgery with no perioperative deaths. There are 100 patients in each group after PSM. The RATS group had an advantage in terms of intraoperative bleeding, postoperative drainage time, postoperative hospital stay, number of lymph nodes cleared (6, 10, 11 stations), number of lymph nodes cleared, and escalation of lymph node pathological stage (cN0 to pN1). In terms of total postoperative chest drainage and hospital costs, the VATS group was more advantageous. There was no statistically significant difference between the two groups in terms of operative time, postoperative complications and intraoperative intermediate chest opening (P>0.05).
Conclusion: RATS has technical and short-term efficacy advantages, especially in lymph node dissection, but has the disadvantage of expensive.
Involvement of the excretory/secretory and surface-associated antigens of Dirofilaria immitis adult worms in the angiogenic response in an in-vitro endothelial cell model
Authors: Machado Cristian David Cardona , Alarcón-Torrecillas Claudia , Pericacho Miguel , Rodríguez-Escolar Iván , Carretón Elena , Montoya-Alonso José Alberto , García Rodrigo Morchón
Published: February 2023

Background: Angiogenesis is a process by which new vessels are formed from pre-existing ones when the physiological conditions of the vascular endothelium are altered. Heartworm disease (Dirofilaria immitis) causes changes in the vascular endothelium of the pulmonary arteries due to obstruction, friction and hypoxia. The aim of this study was to analyze whether the excretory/secretory antigen of adult worms interacts and modulates the angiogenic mechanism, viable cell number and cell migration, as well as the formation of pseudo-capillaries.
Methods: Cultures of human vascular endothelial cells (HUVECs) stimulated with excretory/secretory antigens (DiES), surface-associated antigens (Cut) from D. immitis adult worms, VEGF, as well as DiES+VEGF and Cut+VEGF were used. The production of VEGF-A and other proangiogenic [soluble VEGFR-2 (sVEGFR-2), membrane Endoglin (mEndoglin)] and antiangiogenic [VEGFR-1/soluble Flt (sFlt), soluble Endoglin (sEndoglin)] molecules was assessed using commercial ELISA kits. Cell viability was analyzed by live cell count and cytotoxicity assays by a commercial kit. In addition, viable cell number by MTT-based assay, cell migration by wound-healing assay carrying out scratched wounds, and the capacity of pseudo-capillary formation to analyze cell connections and cell groups in Matrigel cell cultures, were evaluated. In all cases, non‑stimulated cultures were used as controls.
Results: DiES+VEGF and Cut+VEGF significantly increased the production of VEGF and VEGFR2, and only Cut+VEGF significantly increased the production of VEGFR1/sFlt compared to other groups and non-stimulated cultures. Moreover, only DiES+VEGF produced a significant increase in viable cell number and cell migration, as well as in the organization and number of cell connections.
Conclusions: Excretory/secretory and surface-associated antigens of adult D. immitis activated the angiogenic mechanism by mainly stimulating the synthesis of proangiogenic factors, and only excretory/secretory antigens increased viable cell number, activated cell migration and the formation of pseudo-capillaries. These processes could lead to vascular endothelial remodeling of the infected host and favor the long-term survival of the parasite.
The composition of Gut microflora of stingless honey bee (Apidae: Tetragonula spp.) from Dharangaon taluka of Jalgaon district
Authors: Nikam Varun B. , Chopda Manojkumar. Z. , Salunkhe Hemant. P. , Mahajan Namrata G. , Talele Bharati D. , Sonawane Deveshri S. , Maheshwari Nayan R.
Published: February 2023

Stingless bees are the main pollinators in tropical regions because they entirely rely on flowers for nectars and pollen to feed their larvae. They help in pollinating many forests and are valued pollinators in biodiversity. Tetragonula /Trigona is known as the largest genera of stingless bees comprising 32 species worldwide and (meliponini) is by far the largest group of eusocial bees on earth with more than 500 described species and 43 species occurring in the Asian region. In a sterile environment, we surface-sterilized the bees in 75% alcohol for 20 s and then washed them with sterile water. Finally, the entire gut was removed from the abdomen of the bee. The sample was homogenized with one ml of Insect saline solution. 16S RNA analysis was performed by outsourcing service from the Rajiv Gandhi Center for Biotechnology, Thiruvananthapuram. The results showed that the gut of stingless bees contained the bacteria of the Priestia genus
Choosing to Lie: Strategic and Pathological Deception
Authors: Corlett Philip , Rossi-Goldthorpe Rosa
Published: February 2023

Humans lie to one another frequently with local and global consequences. Here we examine and operationalize the choice to lie using a mathematical model which we fit to participant behavior. This model combines perceptions of exploitability and fairness attitudes to explore the interaction between two types of utility: instrumental utility and moral utility. These utilities differed in concert with individual differences in participants’ behavioral traits, and those differences underwrote lies with distinct motivations. Strategic deception – wherein the lie benefits the individual - was common amongst people with elevated subclinical psychopathic traits in the absence of paranoia. It was driven by differences in instrumental utility. Whereas pathological deception – which has no apparent economic or strategic benefit – was common among highly paranoid participants (who also had elevated scores on dark triad personality traits) and was governed by the moral utility of the lie. They lied for little apparent benefit because they failed to modulate their fairness attitudes in line with the decision context. These data challenge accounts of deception that suggest deceiving oneself is a prerequisite to successful lying. They also oppose accounts of psychopathology that connect psychopathic traits to pathological lying, and theories that implicate strategic social decision-making dysfunction in paranoia. More broadly, these data highlight the importance of individual differences in efforts to mitigate deception, which have thus far, largely focused on incentive structures and decision architectures.
ACCOUNTING AND ANALYTICAL ENSURING THE TRANSPORT LOGISTICS MANAGEMENT OF THE ENTERPRISE
Authors: Ovcharova Nataliia , Oleksich Zhanna , Ustyk Yevheniia
Published: February 2023

In current conditions, questions regarding the modeling of accounting and analytical support. In the states of traditional approaches, the basis for analyzing transport and logistics processes is mainly financial and statistical reporting of the enterprise. The purpose of the study is to determine the theoretical and practical aspects of accounting and to develop recommendations for improving the accounting system and analytical support to make managerial decisions in the field of transport logistics. During the implementation of the research task, methods of analysis and synthesis were used to analyze regulatory requirements for the formation of accounting and analytical support for transport logistics management. Methods of analysis and synthesis were used to determine problematic aspects and further directions for improving accounting and analytical support induction and deduction and for formulating conclusions - strategies of logical analysis.
The article examines the theoretical and practical aspects of forming accounting and analytical support for preparing reports on the enterprise's transport logistics management. Analysis of regulatory and legal approval, composition, and primary accounting of transport and logistics costs made it possible to outline their features in various business processes. It was determined that the peculiarities of each business process determine the need for their distribution among the spheres of activity and further determination of the share of costs, which will form such elements as sales costs, the cost of purchased goods, and the cost of services.
Based on the research, a Conceptual model of building a system of accounts for management accounting of transport and logistics costs was formed. The accumulation and distribution of the expenses in terms of the proposed elements will make it possible to collect information for a more detailed analysis of the indicators of internal business processes and their impact on the further development of enterprises. For practical implementation of the model and strengthening accounting analytics for crucial indicators of transport and logistics activity, a structure of accounting accounts for strategic management of transport and logistics costs is proposed. The practical application of the developed proposals will allow an effective information system to help the leadership of different levels of management to obtain the necessary data for decision-making on time.
Effect of Complex Trace Minerals on Reproductive Performance of Beef and Dairy Cattle
Authors: Swenson C. K. , Socha M. T. , Johnson A. B. , Schugel L. M.
Published: February 2023

Providing balanced nutrients is critical for maintaining cow fertility. Adequate trace mineral intake and absorption is required for proper functioning of many metabolic processes associated with reproduction. Reproductive performance of cattle may be compromised if zinc, copper, or manganese status is in the marginal-to-deficient range. Common copper deficiency symptoms in cattle include delayed or suppressed estrus, decreased conception, infertility and embryo death. Inadequate zinc levels have been associated with decreased fertility, abnormal estrus, abortion and altered myometrial contractibility with prolonged labor. Manganese deficiency in cows results in suppression of conception rates, delayed estrus in both post-partum females and young prepuberal heifers, infertility, abortion, immature ovaries and dystocia. This study evaluated the effect on reproduction from feeding complex trace to beef cattle.
Outbreaks of Severe Acute BVDV in Quebec, Ontario and New York State, Occurring Between 1993 and 1995, Linked to the Same Strain of Type 2 BVDV
Authors: Ridpath Julia F. , Neill John D.
Published: February 2023

In this study we compared the virulence and genetic sequence of  four BVDV strains to each other, and to strains isolated in North America from 1989 to 1996.
Reproducible Challenge Model for BVDV Vaccine Efficacy Studies
Authors: Ridpath J. , Neill J. , Becerra V. , Eernisse K. , Gatewood D.
Published: February 2023

In this study we provide documentation of the clinical presentation following infection with a highly virulent type 2 BVDV.
Pregnancy Rates of Recipient Animals Following Administration of a Prostaglandin F2α Receptor Antagonist to Collection Medium during Embryo Recovery
Authors: Prado T. M. , Scenna F. N. , Edwards J. L. , Roper D. A. , Schrick F. N.
Published: February 2023

Previous research has indicated that addition of a PGF2α receptor (FPr) antagonist to culture medium prevented the detrimental action of PGF2α on embryo development. The aim of this study was to evaluate addition of a FPr antagonist to collection medium on pregnancy rates of recipient animals.
Survival of Mycobacterium avium subspecies paratuberculosis in Biofilms on Livestock Watering Trough Materials
Authors: Britt J. S. , Cook K. L. , Rothrock M. J. , Sorrell J. K.
Published: February 2023

Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) is the causative agent of Johne's disease, a chronic enteric infection that affects ruminants. Despite the ubiquitous occurrence of Mycobacterium spp in nature, and the fact that Johne's disease has been reported worldwide, little research has been done to assess its survival in agricultural environments. The goal of this study was to evaluate the ability of M. paratuberculosis to become established and persist in biofilms on coupons composed of common livestock watering trough materials.
Elimination Kinetics of Tilmicosin Following Intramammary Administration in Lactating Dairy Cattle
Authors: Smith G. W. , Davis J. L. , Baynes R. E. , Riviere J. E.
Published: February 2023

Tilmicosin is a macrolide antibiotic approved for both the treatment and control of respiratory disease in beef cattle and dairy cattle under 20 months of age. The drug is not approved for use in lactating dairy cattle; however, tilmicosin is sometimes used in an extralabel manner by bovine practitioners for the intramammary treatment of mastitis. This practice results in detectible residues and has resulted in several instances of milk residue violation referred to the Food Animal Residue Avoidance Databank (FARAD). The purpose of this study was to determine the elimination kinetics of tilmicosin in milk following an intramammary infusion so an appropriate proper milk withdrawal interval could be determined.
Effect of Nitazoxanide on Cryptosporidiosis in Experimentally Challenged Neonatal Dairy Calves
Authors: Ollivett T. L. , Nydam D. V. , Bowman D. D. , Zambriski J. A. , Bellosa M. L. , Linden T. C.
Published: February 2023

Cryptosporidium is a zoonotic protozoan that is most often diagnosed in association with diarrhea in one to three week old dairy calves. There are neither consistently effective nor approved antimicrobials for treatment in animals. The objective of this study was to test nitazoxanide (NTZ) as a treatment for cryptosporidiosis in experimentally challenged dairy calves.
Serum Electrolyte Concentrations Following Intravenous Administration of a 50% Dextrose Solution Bolus to Fresh Dairy Cows
Authors: Wagner Sarah A. , Schimek Daniel E.
Published: February 2023

Ketosis in postpartum dairy cows is often treated by intravenous administration of 50% dextrose solution. Intravenous administration of dextrose to humans increases urinary excretion of calcium, potassium, magnesium, and phosphorus. Low blood levels of these electrolytes have been associated with recumbency in dairy cows. The objective of this study was to determine whether intravenous bolus treatment with 50% dextrose solution would decrease serum electrolyte levels in postpartum Holstein cows.
Survey of Large Animal Veterinarians' Biosecurity Practices
Authors: Anderson D. E. , Silviera F.
Published: February 2023

Recommendations for biosecurity practices are often aimed at idealized scenarios. Although it is well understood that biosecurity protocols must be individualized to account for farm and veterinary specific issues, core principles should be universally applicable. We hypothesized that biosecurity protocols used in field settings by veterinarians in private practice would have similarities that would be useful in bringing these core principle into focus. This study was undertaken in an attempt to gain insight into current "standards of practice" regarding biosecurity protocols used by practicing veterinarians working on farms.
Retrospective Study of 'Jejunal Hemorrhage Syndrome'
Authors: Rademacher G. , Lorenz I. , Hänichen T. , Klee W.
Published: February 2023

A disorder of adult cattle usually referred to as 'jejunal hemorrhage syndrome' has received an increasing amount of attention during the last decade. The frequency of this condition among patients of our clinic has increased. The disease is now the most frequent cause of small intestinal ileus. Therefore, a description of the clinical findings, surgical procedures, and outcomes seemed indicated, especially since the prognosis has been described as poor in the literature.