bluuebunny/crossref_metadata_2025 huggingface dataset:
Sorafenib Versus Lenvatinib-Based Sequential Systemic Therapy for Advanced Hepatocellular Carcinoma: A Real-World Analysis
Authors: Leyh Catherine , Ehmer Ursula , Roessler Daniel , Philipp Alexander B. , Reiter Florian P. , Jeliazkova Petia , Jochheim Leonie S. , Jeschke Matthias , Hammig Janina , Ludwig Johannes M. , Theysohn Jens M. , Geier Andreas , Lange Christian M.
Published: April 2022

The optimal treatment sequence of tyrosine kinase inhibitor (TKI)-based therapy in patients with hepatocellular carcinoma (HCC) remains unclear. Therefore, sequential systemic therapy after first-line therapy with sorafenib or lenvatinib was compared in a retrospective real-world cohort. In total, 164 patients with HCC were included. Child B cirrhosis was present in 26 patients (16.5%), whereas 132 patients (83.5%) had preserved liver function. In total, 72 patients (44%) discontinued systemic therapy after first-line therapy while 51 (31%) and 31 (19%) patients received 2 or more treatment lines. Most notably, median overall survival (mOS) was influenced by liver functional status and patient performance status at the beginning of first-line therapy. Patients receiving a sequential therapy regimen had significantly longer mOS compared to patients that discontinued systemic therapy after omitting first-line treatment. The choice of the initial TKI did not impact mOS. A clear deterioration of liver function could be observed during the course of TKI-based treatment.
Epizoic Rotifers and Microcrustaceans on Bivalves of Different Size and Behavior
Authors: Ejsmont-Karabin Jolanta , Karpowicz Maciej , Feniova Irina
Published: April 2022

Previous mesocosm experiments with the epizoon of Dreissena polymorpha revealed that the communities of Rotifera and Crustacea were much more abundant and had higher species richness than epizoon of Unio tumidus in nature. These differences could be attributed to different environmental conditions and/or different host behavior. To test this hypothesis, we compared epizoon of D.polymorpha and U.tumidus placed in identical mesocosm conditions, in which Unio could not move vertically due to the lack of sediments. Half of the mesocosms contained D. polymorpha, the other half comprised U. tumidus. Each species of mollusks was kept in the mesocosms with eutrophic and mesotrophic conditions. Finally, we established four treatments that were replicated in triplicate mesocosms. Mesocosm experiments showed that epizoon communities of U. tumidus were even more abundant than that of D. polymorpha and their species richness was similar. Therefore, we concluded that previously revealed strong differences between epizoon communities of D. polymorpha and U. tumidus were related to the different environmental conditions and bivalve behavior.
Design of Cyber-Physical Waste Management Systems Focusing on Energy Efficiency and Sustainability
Authors: Akkad Mohammad Zaher , Haidar Sameh , Bányai Tamás
Published: April 2022

Industry 4.0 tools, such as the Internet of Things, artificial intelligence, digital twinning, and cloud computing, create a technological revolution that accelerates efforts to optimize the efficiency of cyber-physical operations and services. The waste management system requires a critical share of city logistics optimization, especially when using cyber-physical systems. Modern tools reduce the required municipal waste handling, such as loading and unloading, transportation, and warehousing, which leads to an increase in efficiency and flexibility, saving energy and time, and protecting the environment. In this paper, we present a cyber-physical waste management system solution by providing a cyber-physical model design and description, mathematical modeling, and two cases to investigate the impact on energy consumption and emissions. After an introduction and literature review, we describe the design of the cyber-physical model and tackle the first echelon. The designed system incorporates the IoT, smart bins with multi-percentage sensors, data and information analysis, vehicles’ actual routes, energy and emissions optimization, multi-echelon systems, time windows, and flexibility. Mathematical modeling equations for the optimized total energy consumption are presented. Thirty and twenty smart bins located in VIII District in Budapest are detailed as two case studies, where solutions for the optimized real routes and energy consumption are found using three metaheuristic algorithms: genetic, particle swarm, and simulated annealing optimization algorithms. The accrued emissions of CO, NMHC, CH4, NOx, and PM for the optimized solutions are calculated. Finally, the results are compared with a random traditional solution to measure the effectiveness.
A Dynamic Autocatalytic Network Model of Therapeutic Change
Authors: Ganesh Kirthana , Gabora Liane
Published: April 2022

Psychotherapy involves the modification of a client’s worldview to reduce distress and enhance well-being. We take a human dynamical systems approach to modeling this process, using Reflexively Autocatalytic foodset-derived (RAF) networks. RAFs have been used to model the self-organization of adaptive networks associated with the origin and early evolution of both biological life, as well as the evolution and development of the kind of cognitive structure necessary for cultural evolution. The RAF approach is applicable in these seemingly disparate cases because it provides a theoretical framework for formally describing under what conditions systems composed of elements that interact and ‘catalyze’ the formation of new elements collectively become integrated wholes. In our application, the elements are mental representations, and the whole is a conceptual network. The initial components—referred to as foodset items—are mental representations that are innate, or were acquired through social learning or individual learning (of pre-existing information). The new elements—referred to as foodset-derived items—are mental representations that result from creative thought (resulting in new information). In clinical psychology, a client’s distress may be due to, or exacerbated by, one or more beliefs that diminish self-esteem. Such beliefs may be formed and sustained through distorted thinking, and the tendency to interpret ambiguous events as confirmation of these beliefs. We view psychotherapy as a creative collaborative process between therapist and client, in which the output is not an artwork or invention but a more well-adapted worldview and approach to life on the part of the client. In this paper, we model a hypothetical albeit representative example of the formation and dissolution of such beliefs over the course of a therapist–client interaction using RAF networks. We show how the therapist is able to elicit this worldview from the client and create a conceptualization of the client’s concerns. We then formally demonstrate four distinct ways in which the therapist is able to facilitate change in the client’s worldview: (1) challenging the client’s negative interpretations of events, (2) providing direct evidence that runs contrary to and counteracts the client’s distressing beliefs, (3) using self-disclosure to provide examples of strategies one can use to diffuse a negative conclusion, and (4) reinforcing the client’s attempts to assimilate such strategies into their own ways of thinking. We then discuss the implications of such an approach to expanding our knowledge of the development of mental health concerns and the trajectory of the therapeutic change.
Electrodeposition and Micro-Mechanical Property Characterization of Nickel–Cobalt Alloys toward Design of MEMS Components
Authors: Jiang Yiming , Chen Chun-Yi , Luo Xun , Yamane Daisuke , Mizoguchi Masanori , Kudo Osamu , Maeda Ryu , Sone Masato , Chang Tso-Fu Mark
Published: April 2022

Nickel–cobalt alloys were prepared by alloy electrodeposition with a sulfamate bath, and the mechanical properties on the micro-scale were evaluated for the application as micro-components in miniaturized electronic devices. Nickel bromide and a commercially available surface brightener were used as the additives. The cobalt content increased from 21.5 to 60.1 at.% after addition of nickel bromide into the bath, and the grain size refined from 21.1 to 13.2 nm when the surface brightener was used. The mechanical properties on the micro-scale were evaluated by micro-compression test using micro-pillar type specimens fabricated by a focused ion beam system to take the sample size effect into consideration. The yield strength of the nickel–cobalt alloy having an average grain size at 13.9 nm and cobalt content of 66.6 at.% reached 2.37 GPa, revealing influences from the sample size, grain boundary strengthening, and solid solution strengthening effects.
Representative Group Decision-Making in Forest Management: A Compromise Approach
Authors: Ortiz-Urbina Esther , Diaz-Balteiro Luis , Pardos Marta , González-Pachón Jacinto
Published: April 2022

The correct aggregation of stakeholders’ preferences is a vital aspect of solving problems associated with natural resources. In fact, there is no one solution that permits the incorporation of those preferences into techniques that, in turn, address multiple objectives in the management of those resources. In this context, this work aims to assign, analyse, and compare the weights of importance to groups of stakeholders (representativity) starting from different approaches and methodologies: pairwise comparison matrices (using a subjective approach) and the voting power notion (when an objective approach is deployed). For the latter, a variant of the extended goal programming model is employed. The results show different weight values and, therefore, scenarios, in which the social groups defined acquire diverse importance. It is also observed that there are scenarios determined by different values of the control parameter, in which the results of the two above-mentioned approaches are similar. Finally, it is demonstrated how the affiliation of stakeholders to other social groups (different identities) affects the results obtained.
Evaluation of a Four-Gene Panel for Hereditary Cancer Risk Assessment
Authors: Secondino Angela , Starnone Flavio , Veneruso Iolanda , Di Tella Maria , Conato Serena , De Angelis Carmine , De Placido Sabino , D’Argenio Valeria
Published: April 2022

BRCA1/2 are tumor suppressor genes involved in DNA double-strand break repair. They are the most penetrant genes for hereditary breast and ovarian cancers, but pathogenic variants in these two genes can be identified only in a fraction of hereditary cases. Following the diffusion of BRCA molecular testing and the availability of specific therapeutic strategies for the management of pathogenic variant carriers, the demand for the analysis of additional predisposing genetic factors has increased. Indeed, there is accumulating evidence regarding the role of other genes, including CHEK2 and PALB2. Both of them are involved in the same molecular pathway as BRCA genes, with CHEK2 being responsible for cell cycle stopping to allow the repair of DNA double-strand breaks and PALB2 being able to interact with BRCA1 and activate BRCA2. Thus, their role as additional hereditary cancer predisposing factors is intriguing. Accordingly, guidelines for hereditary cancer risk assessment have been updated to include the criteria for additional genes testing. In this context, we validated a commercially available kit allowing for the simultaneous analysis of BRCA1, BRCA2, CHEK2 and PALB2. Forty-eight patients, already tested for BRCA mutational status, were re-analyzed in the present study. Results comparison showed that the tested method was able to correctly identify all the variants previously detected in the same patients. In particular, all single-nucleotide variants and small indels were correctly identified. Moreover, two copy number variants, included to assess the software’s performance in detecting this kind of gene alteration, were also detected. Even if copy number variant estimation still requires confirmation by a molecular technique to avoid false positive results, it is able to reduce the number of patients requiring multiplex ligation probe amplification analysis, positively impacting the test’s turnaround time. Finally, since the time and costs of the analysis are similar to those required just for BRCA genes, this strategy may be affordable for providing a more comprehensive test for hereditary cancer risk assessment.
New Early Cretaceous Geosites with Palaeogeographical Value from the Northwestern Caucasus
Authors: Ruban Dmitry A. , Tolokonnikova Zoya A.
Published: April 2022

Field investigations in the northwestern segment of the Greater Caucasus, a Late Cenozoic orogen, have permitted the establishment of two new geosites, namely the Ubin and Bezeps geosites. Both represent Berriasian–Middle Valanginian (Early Cretaceous) marine deposits with abundant trace fossils. The latter are attributed to the Nereites ichnofacies and indicate on deep marine palaeoenvironments (this interpretation challenges previous reconstructions). The geosites represent the palaeogeographical type of geoheritage. They are characterized, particularly, by high scientific and aesthetic importance, but restricted accessibility. Further geoheritage inventory in the central Northwestern Caucasus seems to be promising.
Generalized Tetanus in a Canadian Farmer Following Orthopedic Surgery
Authors: Chauhan Utkarsh , Ghimire Anukul , Raval Milan , Boyington Curtiss , Haponiuk Adrienne , Koller Gregory , Korzan Jeffrey , Yacyshyn Elaine
Published: April 2022

Tetanus is extremely rare in developed countries. We report the first documented case of tetanus in the province of Alberta since 2016: a farmer that developed trismus, shoulder stiffness, and fevers eight days following orthopedic surgery. Tetanus immunoglobulin elicited rapid recovery. We highlight risk factors, pathogenesis, epidemiology, and diagnostic challenges.
Individualized Analysis of Lateral Asymmetry Using Hip-Knee Angular Measures in Soccer Players: A New Methodological Perspective of Assessment for Lower Limb Asymmetry
Authors: García-García Oscar , Molina-Cárdenas Ángela , Álvarez-Yates Tania , Iglesias-Caamaño Mario , Serrano-Gómez Virginia
Published: April 2022

This study aimed to: (1) determine the magnitude and direction of lateral asymmetry in well-trained soccer players using hip and knee ROM tests; (2) inquire if asymmetry relies on the ROM test performed and/or gender; and (3) establish asymmetry thresholds for each ROM test to individualize lower-limbs asymmetry. One hundred amateur soccer players were assessed using hip–knee ROM tests: Straight Leg Raise, modified Thomas Test, hip internal rotation and external rotation, hip abduction (ABD) and adduction (ADD), Nachlas Test and Rigde Test. There are significant differences between tests when determining the magnitude of lateral asymmetry (F = 3.451; p = 0.001; ηp2 = 0.031) without significant differences between gender (F = 0.204; p = 0.651; ηp2 = 0.001). Asymmetry threshold results differ significantly between using a fixed or a specific threshold (F = 65.966; p = 0.001; ηp2 = 0.985). All tests indicate that the direction of asymmetry is towards the dominant limb. In conclusion, the ROM test used determines the magnitude and direction of the lateral asymmetry of the amateur soccer players. The ABD and ADD are the ROM tests that showed higher percentages of asymmetry, without differences between female and male soccer players. Using a specific asymmetry threshold formula can classify more players as asymmetrical than with a fixed threshold.
Syndromic Surveillance Systems for Mass Gatherings: A Scoping Review
Authors: Spector Eliot , Zhang Yahan , Guo Yi , Bost Sarah , Yang Xi , Prosperi Mattia , Wu Yonghui , Shao Hui , Bian Jiang
Published: April 2022

Syndromic surveillance involves the near-real-time collection of data from a potential multitude of sources to detect outbreaks of disease or adverse health events earlier than traditional forms of public health surveillance. The purpose of the present study is to elucidate the role of syndromic surveillance during mass gathering scenarios. In the present review, the use of syndromic surveillance for mass gathering scenarios is described, including characteristics such as methodologies of data collection and analysis, degree of preparation and collaboration, and the degree to which prior surveillance infrastructure is utilized. Nineteen publications were included for data extraction. The most common data source for the included syndromic surveillance systems was emergency departments, with first aid stations and event-based clinics also present. Data were often collected using custom reporting forms. While syndromic surveillance can potentially serve as a method of informing public health policy regarding specific mass gatherings based on the profile of syndromes ascertained, the present review does not indicate that this form of surveillance is a reliable method of detecting potentially critical public health events during mass gathering scenarios.
Examining Older Adults’ Home Functioning Using the American Housing Survey
Authors: Lee Mi Jung , Kim Daejin , Romero Sergio , Hong Ickpyo , Bliznyuk Nikolay , Velozo Craig
Published: April 2022

Identifying individuals at risk of experiencing functional difficulty at home would support timely home safety assessment and modification services, which could lead to reducing home incidents such as falls. The objective of this study was to calculate older adults’ functional difficulty at home scores using the 12 physical function items in the American Housing Survey National and Metropolitan Data (AHS). Among the 28,474 older adults selected for this study, we used 19,932 for measurement model development and 8542 for model testing. Confirmatory factor analysis confirmed an adequate fit of the one-dimensional model with all AHS 12 items loading on one latent construct (functional difficulty at home) (RMSEA: 0.034, CFI: 0.990, and TLI: 0.988). Based on our model selection process, we determined that the Graded Response Model was an optimal model for our analysis and separated two detected differential functioning items for each sex. Using the testing dataset, we validated that the estimated functional difficulty scores showed an expected item hierarchy and statistically significant differences in their association with housing and demographic conditions (p < 0.001). Our results demonstrated the process of using the 12 AHS physical function at home items to produce validated scores of older adults’ functional difficulty at home.
Association between Healthy Eating Index and Mental Health in Middle-Aged Adults Based on Household Size in Korea
Authors: Kim Ji-Myung , Lee EunJung
Published: April 2022

This study examined the association between dietary lifestyle and mental health outcomes among middle-aged individuals (40–60-year-olds) living alone, using the Korean Healthy Eating Index (KHEI). The participants were selected (1334 men and 2185 women) from the Korea National Health and Nutrition Examination Survey 2016–2018 and categorized into single/multi-person households. The KHEI scores were calculated based on the 24 h recall data of dietary intake. Among both sexes, single-person households had significantly lower subjective health status scores (p = 0.015 for men; p < 0.001 for women), lower EuroQol-5D index scores (p = 0.011 for men; p = 0.003 for women), and higher Patient Health Questionnaire-9 index scores (p = 0.004 for men; p < 0.001 for women), compared to multi-person households. Men had a higher risk of depression in single-person households compared to multi-person households (OR, 3.5; 95% CI, 1.2–10.1). For women, the ORs for stress perception with the first tertile group of KHEI scores in single-person and multi-person households compared to the third tertile group were 3.5 (95% CI, 1.3–9.0) and 1.4 (95% CI, 1.0–1.8), respectively. The results of this study are expected to be used as baseline data to establish nutrition, healthy eating behavior, and health policies for the middle-aged living alone.
Ability of Wearable Accelerometers-Based Measures to Assess the Stability of Working Postures
Authors: Guo Liangjie , Kou Junhui , Wu Mingyu
Published: April 2022

With the rapid development and widespread application of wearable inertial sensors in the field of human motion capture, the low-cost and non-invasive accelerometer (ACC) based measures have been widely used for working postural stability assessment. This study systematically investigated the abilities of ACC-based measures to assess the stability of working postures in terms of the ability to detect the effects of work-related factors and the ability to classify stable and unstable working postures. Thirty young males participated in this study and performed twenty-four load-holding tasks (six working postures × two standing surfaces × two holding loads), and forty-three ACC-based measures were derived from the ACC data obtained by using a 17 inertial sensors-based motion capture system. ANOVAs, t-tests and machine learning (ML) methods were adopted to study the factors’ effects detection ability and the postural stability classification ability. The results show that almost all forty-three ACC-based measures could (p < 0.05) detect the main effects of Working Posture and Load Carriage, and their interaction effects. However, most of them failed in (p ≥ 0.05) detecting Standing Surface’s main or interaction effects. Five measures could detect both main and interaction effects of all the three factors, which are recommended for working postural stability assessment. The performance in postural stability classification based on ML was also good, and the feature set exerted a greater influence on the classification accuracy than sensor configuration (i.e., sensor placement locations). The results show that the pelvis and lower legs are recommended locations overall, in which the pelvis is the first choice. The findings of this study have proved that wearable ACC-based measures could assess the stability of working postures, including the work-related factors’ effects detection ability and stable-unstable working postures classification ability. However, researchers should pay more attention to the measure selection, sensors placement, feature selection and extraction in practical applications.
Healthcare Professionals’ Compliance with the Standard Management Guidelines towards the Use of Biological Disease-Modifying Anti-Rheumatic Drugs in Rheumatoid Arthritis Patients
Authors: Shakeel Sadia , Iffat Wajiha , Qamar Ambreen , Rehman Hina , Ghuman Faiza , Butt Fareeha , ur Rehman Anees , Madléna Melinda , Paulik Edit , Gajdács Márió , Jamshed Shazia
Published: April 2022

Treatment of rheumatoid arthritis (RA) is complicated, with numerous aspects influencing decision-making, including disease severity, comorbidities, and patient preferences. The present study aimed to evaluate healthcare professionals’ (HCPs) knowledge of biological disease-modifying anti-rheumatic drugs (bDMARDs) and their compliance with the standard management guidelines for assuring optimal RA therapy. The cross-sectional, survey-based study was performed in various healthcare and academic settings in Karachi, Pakistan to probe HCPs’ knowledge of bDMARDs and their compliance with the European League against Rheumatism (EULAR) recommendations for the management of RA patients. Overall, n = 413 questionnaires were included in our study (response rate: 82.6%). The physicians were further well-informed about the indications (n = 276, 91.3%, p = 0.001) and monitoring requirements (n = 258, 85.4%, p = 0.004). The pharmacists were more knowledgeable about the drug targets (n = 96, 86.4%, p = 0.029) and their mechanisms of action (n = 80, 72.0%, p = 0.013). Male respondents as compared with females (41.3% vs. 35.6%, p = 0.04), and physicians as compared with pharmacists (40.7% vs. 37.8%, p = 0.012), were more confident in using bDMARDs than conventional treatment in RA patients. Our findings show that the respondents were familiar with the attributes of bDMARDs and the standard management guidelines for RA care. Our results may be relevant in creating new methods, guidelines, and treatments to enhance RA treatment adherence, satisfaction, and health outcomes.
Happiness in University Students: Personal, Familial, and Social Factors: A Cross-Sectional Questionnaire Survey
Authors: Jiang Yingying , Lu Chan , Chen Jing , Miao Yufeng , Li Yuguo , Deng Qihong
Published: April 2022

Happiness is the foundation of a better life and a goal that people pursue; however, happiness levels among university students are low. The purpose of this study is to explore the main factors influencing student happiness. A nationwide cross-sectional study was conducted in China in 2020. Data on student happiness was collected using the Oxford Happiness Questionnaire, and students’ personal, familial, and social information were obtained using another questionnaire. Logistic regression analysis was employed to examine the association between student happiness and these factors in terms of odds ratio (OR) and 95% confidence interval (CI). A total of 2186 valid questionnaires were obtained. Firstly, student happiness was found to be associated with personal factors. The results found that happiness was significantly associated with state of health, the adjusted OR (95% CI) = 3.41 (2.01–5.79) for healthy students compared to unhealthy students, and that happiness decreased with the student’s age (OR = 0.79 and 95% CI = 0.63–0.98). Secondly, the research suggested that happiness was associated with familial factors. Both frequent contact with family and a harmonious relationship with parents significantly enhanced happiness with ORs (95% CIs) 1.42 (1.17–1.71) and 2.32 (1.83–2.95), respectively. Thirdly, student happiness was associated with several social factors. Students who performed well academically, who went to sleep early, and who were in a loving relationship were found to be happier than those with poor academic performance, went to sleep late, and who were single, for which the ORs (95% CIs) were, respectively, 1.87 (1.51–2.32), 1.50 (1.24–1.81), and 1.32 (1.09–1.60). The survey identified several key personal, familial, and social factors influencing university student happiness, which can provide an effective measure to improve their happiness.
Effects of Yogurt Intake on Cardiovascular Strain during Outdoor Interval Walking Training by Older People in Midsummer: A Randomized Controlled Study
Authors: Uchida Koji , Shimamura Ryutaro , Ikefuchi Ryo , Morikawa Mayuko , Furihata Mayuka , Hanaoka Masaaki , Nose Hiroshi , Masuki Shizue
Published: April 2022

We examined whether post-exercise yogurt intake reduced cardiovascular strain during outdoor interval walking training (IWT) in older people during midsummer. The IWT is a training regimen repeating slow and fast walking at ~40% and ≥70% peak aerobic capacity, respectively, for 3 min each per set, ≥5 sets per day, and ≥4 days/wk. We randomly divided 28 male and 75 female older people (~73 yr), who had performed IWT ≥12 months, into a carbohydrate group (CHO-G) consuming jelly (45 g CHO, 180 kcal) and a yogurt group (YGT-G) consuming a yogurt drink (9.3 g protein, 39 g CHO, 192 kcal) immediately after daily IWT for 56 days while monitoring exercise intensity and heart rate (HR) with portable devices. We analyzed the results in 39 subjects for the CHO-G and 37 subjects for the YGT-G who performed IWT ≥ 4 days/wk, ≥60 min total fast walking/wk, and ≥4 sets of each walk/day. We found that the mean HR for fast walking decreased significantly from the baseline after the 30th day in the YGT-G (p < 0.03), but not in the CHO-G (p = 1.00). There were no significant differences in training achievements between the groups. Thus, post-exercise yogurt intake might reduce cardiovascular strain during outdoor walking training in older people.
Factors Affecting the Quality of Work Life of Nurses at Tertiary General Hospitals in the Context of the COVID-19 Pandemic
Authors: Hwang Eunhee
Published: April 2022

The prolonged coronavirus disease 2019 (COVID-19) pandemic has caused an overload of work for nurses and resulted in high levels of stress. Improving the quality of work life may be a useful mediator for these demands. The purpose of this study was to identify factors affecting work stress, turnover intention, mindfulness, and quality of work life in nurses working in tertiary general hospitals. The participants of this study were 207 female nurses working in tertiary general hospitals with more than six months of clinical experience. Data were collected using an online Google survey. Data were analyzed using the SPSS/WIN 26.0 program. The mean score for the quality of work life was 3.81 ± 0.53 out of six points. The quality of work life was negatively correlated with job stress (r = −0.36, p < 0.001) and turnover intention (r = −0.45, p < 0.001) and positively correlated with mindfulness (r = 0.35, p < 0.001). Factors affecting quality of work life were work satisfaction (β = 0.27, p = 0.004), job stress (β = −0.23, p < 0.001), and turnover intention (β = −0.18, p = 0.016). As a result, positive factors such as work satisfaction had stronger effects than negative factors. Thus, it would be necessary to seek strategies such as improving compensation for nurses, enhancing teamwork, or establishing a support system for managers, superiors, and colleagues.
Frequency-Dependent Properties of the Hyperpolarization-Activated Cation Current, If, in Adult Mouse Heart Primary Pacemaker Myocytes
Authors: Hu Wei , Clark Robert B. , Giles Wayne R. , Kondo Colleen , Zhang Henggui
Published: April 2022

A number of distinct electrophysiological mechanisms that modulate the myogenic spontaneous pacemaker activity in the sinoatrial node (SAN) of the mammalian heart have been investigated extensively. There is agreement that several (3 or 4) different transmembrane ionic current changes (referred to as the voltage clock) are involved; and that the resulting net current interacts with direct and indirect effects of changes in intracellular Ca2+ (the calcium clock). However, significant uncertainties, and important knowledge gaps, remain concerning the functional roles in SAN spontaneous pacing of many of the individual ion channel- or exchanger-mediated transmembrane current changes. We report results from patch clamp studies and mathematical modeling of the hyperpolarization-activated current, If, in the generation/modulation of the diastolic depolarization, or pacemaker potential, produced by individual myocytes that were enzymatically isolated from the adult mouse sinoatrial node (SAN). Amphotericin-mediated patch microelectrode recordings at 35 °C were made under control conditions and in the presence of 5 or 10 nM isoproterenol (ISO). These sets of results were complemented and integrated with mathematical modeling of the current changes that take place in the range of membrane potentials (−70 to −50 mV), which corresponds to the ‘pacemaker depolarization’ in the adult mouse SAN. Our results reveal a very small, but functionally important, approximately steady-state or time-independent current generated by residual activation of If channels that are expressed in these pacemaker myocytes. Recordings of the pacemaker depolarization and action potential, combined with measurements of changes in If, and the well-known increases in the L-type Ca2+ current, ICaL, demonstrated that ICaL activation, is essential for myogenic pacing. Moreover, after being enhanced (approximately 3-fold) by 5 or 10 nM ISO, ICaL contributes significantly to the positive chronotropic effect. Our mathematical model has been developed in an attempt to better understand the underlying mechanisms for the pacemaker depolarization and action potential in adult mouse SAN myocytes. After being updated with our new experimental data describing If, our simulations reveal a novel functional component of If in adult mouse SAN. Computational work carried out with this model also confirms that in the presence of ISO the residual activation of If and opening of ICaL channels combine to generate a net current change during the slow diastolic depolarization phase that is essential for the observed accelerated pacemaking rate of these SAN myocytes.
Flying without a Net: Space Radiation Cancer Risk Predictions without a Gamma-ray Basis
Authors: Cucinotta Francis A.
Published: April 2022

The biological effects of high linear energy transfer (LET) radiation show both a qualitative and quantitative difference when compared to low-LET radiation. However, models used to estimate risks ignore qualitative differences and involve extensive use of gamma-ray data, including low-LET radiation epidemiology, quality factors (QF), and dose and dose-rate effectiveness factors (DDREF). We consider a risk prediction that avoids gamma-ray data by formulating a track structure model of excess relative risk (ERR) with parameters estimated from animal studies using high-LET radiation. The ERR model is applied with U.S. population cancer data to predict lifetime risks to astronauts. Results for male liver and female breast cancer risk show that the ERR model agrees fairly well with estimates of a QF model on non-targeted effects (NTE) and is about 2-fold higher than the QF model that ignores NTE. For male or female lung cancer risk, the ERR model predicts about a 3-fold and more than 7-fold lower risk compared to the QF models with or without NTE, respectively. We suggest a relative risk approach coupled with improved models of tissue-specific cancers should be pursued to reduce uncertainties in space radiation risk projections. This approach would avoid low-LET uncertainties, while including qualitive effects specific to high-LET radiation.
Study on the Properties and Mechanism of Magnesium Oxychloride Adhesive Particleboard Modified by Fly Ash
Authors: Cui Meng , Zheng Nihua , Wang Shixin , Luo Bin , Liu Hongguang
Published: April 2022

Magnesium oxychloride adhesive (MOA) is a kind of inorganic adhesive with a low energy consumption and environmental protection. The modified particleboard with magnesium oxychloride adhesive (MOPB) has the advantages of no volatiles and has a high mechanical strength. In this study, MOA and poplar shavings were used to prepare MOPB, fly ash (FA) was used to modify and enhance the properties of MOPB; the influence mechanism on the mechanical of MOPB was studied. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) were used to analysis the bonding mechanism of MOA and poplar shavings, and the composite system model of MOPB was constructed. The mechanism of modified MOPB with FA (MOPB-FA) was clarified. The results show that the use of FA effectively improved the mechanical strength of MOPB, and when the ratio of FA addition was 15 wt%, MOPB-FA’s modulus of rupture (MOR) value was 16.32 MPa, an increase of 24.5% more than before (13.11 MPa). The modulus of elasticity (MOE) value was 4595.51 MPa, an increase of 76.7% more than before (2600 MPa), and the thickness of swelling (TS) value was 0.35%, a decrease of 85.2% less than before (2.36%).
Progress in Preparation of Sea Urchin-like Micro-/Nanoparticles
Authors: Ma Ruijing , Xiang Liqin , Zhao Xiaopeng , Yin Jianbo
Published: April 2022

Urchin-like microparticles/nanoparticles assembled from radial nanorods have a good appearance and high specific surface area, providing more exposed active sites and shortening the diffusion path of photoexcited carriers from the interior to the surface. The interfacial interaction and physical and chemical properties of the materials can be improved by the interfacial porous network induced by interlacing nano-branches. In addition, multiple reflections of the layered microstructure can absorb more incident light and improve the photocatalytic performance. Therefore, the synthesis and functionalization of three-dimensional urchin-like nanostructures with controllable size, shape, and hierarchy have attracted extensive attention. This review aims to provide an overview to summarize the structures, mechanism, and application of urchin-like microparticles/nanoparticles derived from diverse synthesis methods and decoration types. Firstly, the synthesis methods of solid urchin-like micro-/nanoparticles are listed, with emphasis on the hydrothermal/solvothermal method and the reaction mechanism of several typical examples. Subsequently, the preparation method of composite urchin-like micro-/nanoparticles is described from the perspective of coating and doping. Then, the research progress of urchin-like hollow microspheres is reviewed from the perspective of the step-by-step method and synchronous method, and the formation mechanism of forming urchin-like hollow microspheres is discussed. Finally, the application progress of sea urchin-like particles in the fields of photocatalysis, electrochemistry, electromagnetic wave absorption, electrorheological, and gas sensors is summarized.
Characterization of Organosolv Lignins and Their Application in the Preparation of Aerogels
Authors: Jõul Piia , Ho Tran T. , Kallavus Urve , Konist Alar , Leiman Kristiina , Salm Olivia-Stella , Kulp Maria , Koel Mihkel , Lukk Tiit
Published: April 2022

The production of novel materials and value-added chemicals from lignin has received considerable attention in recent years. Due to its abundant occurrence in nature, there is a growing interest in utilizing lignin as a feedstock for functional materials production, for example aerogels. Much like in the synthesis of phenol-based resins, the vacant ortho positions of the aromatic rings in lignin can crosslink with formaldehyde and form polymeric gels. After drying the hydrogels with supercritical CO2, highly porous aerogels are obtained. Current study focuses on the preparation and thorough parametrization of organosolv lignins from different types of lignocellulosic biomass (aspen, pine, and barley straw) as well as their utilization for the preparation of lignin-5-methylresorcinol-formaldehyde aerogels. The thorough structural characterization of the obtained aerogels was carried out by gas adsorption, IR spectroscopy, and scanning electron microscopy. The obtained lignin-based monolithic mesoporous aerogels had specific surface areas and total pore volumes in the upward ranges of 450 m2/g and 1.4 cm3/g, respectively.
Mechanical Performance of 3D Printed Concrete in Steam Curing Conditions
Authors: Wang Bolin , Yao Xiaofei , Yang Min , Zhang Runhong , Huang Jizhuo , Wang Xiangyu , Dong Zhejun , Zhao Hongyu
Published: April 2022

Three-dimensional (3D) concrete printing (3DCP) technology attracts significant attention from research and industry. Moreover, adequate mechanical performance is one of the primary properties for materials, meeting the demand of structural safety using 3DCP technology. However, research on curing conditions as the significant influence factor of mechanical capacity is required to accelerate the practical application of 3DCP technology. This study aims to explore the impact of various steam curing conditions (heating rate, constant temperature time, and constant temperature) on the mechanical performance of printed concrete containing solid wastes. Moreover, the optimal steam curing conditions are obtained for compressive, tensile, and flexural properties in different directions. Subsequently, anisotropies in the mechanical properties of printed composites and interlayer bonding behaviors are investigated when various curing conditions are employed. The result shows that steam curing conditions and solid waste incorporation improves the interlayer bond for 3D printed cement-based composites.
Defect Detection Method for CFRP Based on Line Laser Thermography
Authors: Wang Quan , Zhang Zhijie , Yin Wuliang , Chen Haoze , Liu Yushan
Published: April 2022

A continuous line laser scanning inspection technique for tracing load-bearing structures was developed and applied to defect detection of unidirectional carbon-fiber-reinforced polymers for aero engines. The heat transfer model of the material was analyzed using the finite element software COMSOL. Meanwhile, a laser platform was built and an image algorithm was used to verify the feasibility of the method. The potential of this technique for detecting defects and providing information on the location of defects in carbon fiber composites was analyzed. Results indicate line laser thermal imaging can successfully determine the size, location, and crack angle of surface damage with extremely high accuracy. The positioning accuracy error for impact and fracture defects is less than 20%, and the detection rate can reach 100% if the defect is in the special position of just leaving the heating area. The angle detection of fracture cracks can be accurate within 10°.
Does Adherence to the Mediterranean Diet Have a Protective Effect against Asthma and Allergies in Children? A Systematic Review
Authors: Koumpagioti Despoina , Boutopoulou Barbara , Moriki Dafni , Priftis Kostas N. , Douros Konstantinos
Published: April 2022

Dietary pattern may potentially impact on the pathogenesis of asthma and allergies. The Mediterranean Diet (MD) has significant health benefits due to its antioxidant and anti-inflammatory properties. The aim of this systematic review was to investigate the effectiveness of adherence to the MD against asthma and allergies in childhood. Hence, a systematic literature search was conducted on PubMed, ESBCO (Cinahl), Scopus, and the Cochrane Library databases up to 26 January 2022. The total number of articles obtained, after the initial search on the databases was conducted, was 301. Twelve studies were included, after the removal of duplicates and screening for eligibility. Our findings indicated a protective role of the MD against childhood asthma, but they also imply that the MD probably does not affect the development of allergies. Nevertheless, the heterogeneity and limitations of the studies highlight the need for randomized controlled trials that will focus on the pediatric population and hopefully provide more robust evidence.
Geraniol Ameliorates Doxorubicin-Mediated Kidney Injury through Alteration of Antioxidant Status, Inflammation, and Apoptosis: Potential Roles of NF-κB and Nrf2/Ho-1
Authors: AlAsmari Abdullah F. , Ali Nemat , Alharbi Metab , Alqahtani Faleh , Alasmari Fawaz , Almoqbel Daad , AlSwayyed Mohammed , Alshammari Abdulrahman , Alanazi Mohammed M. , Alhoshani Ali , Al-Harbi Naif O.
Published: April 2022

Doxorubicin-mediated kidney impairment is a serious problem in cancer treatment. Accordingly, this work investigated the ability of geraniol to modulate doxorubicin-induced kidney damage using a rat model. Rats were randomly assigned to four groups: control, doxorubicin (20 mg/kg, intraperitoneal, i.p.), doxorubicin plus 100 mg/kg of geraniol, and doxorubicin plus 200 mg/kg of geraniol. A single doxorubicin injection triggered kidney impairment, as evidenced by the altered serum creatinine, blood urea nitrogen, and albumin values; it also caused histological changes in the kidney architecture. Additionally, doxorubicin enhanced lipid peroxidation while lowering reduced glutathione, catalase activity, and the expression of glutathione peroxidase and superoxide dismutase. Interestingly, pre-treatment with geraniol rescued doxorubicin-induced alterations in kidney antioxidant parameters, enzymatic activity, and the expression of inflammatory and apoptosis-mediating gene and proteins. Moreover, prophylactic treatment with geraniol preserved most kidney histological characteristics in a dose-dependent manner. These findings support that geraniol could protect against doxorubicin-mediated kidney dysfunction. However, further research is needed to clarify the mechanisms of geraniol’s protective effects against doxorubicin-mediated kidney dysfunction.
Vitamin D: A Role Also in Long COVID-19?
Authors: Barrea Luigi , Verde Ludovica , Grant William B. , Frias-Toral Evelyn , Sarno Gerardo , Vetrani Claudia , Ceriani Florencia , Garcia-Velasquez Eloisa , Contreras-Briceño José , Savastano Silvia , Colao Annamaria , Muscogiuri Giovanna
Published: April 2022

Coronavirus disease 2019 (COVID-19) has quickly become a global pandemic. Reports from different parts of the world indicate that a significant proportion of people who have recovered from COVID-19 are suffering from various health problems collectively referred to as “long COVID-19”. Common symptoms include fatigue, shortness of breath, cough, joint pain, chest pain, muscle aches, headaches, and so on. Vitamin D is an immunomodulatory hormone with proven efficacy against various upper respiratory tract infections. Vitamin D can inhibit hyperinflammatory reactions and accelerate the healing process in the affected areas, especially in lung tissue. Moreover, vitamin D deficiency has been associated with the severity and mortality of COVID-19 cases, with a high prevalence of hypovitaminosis D found in patients with COVID-19 and acute respiratory failure. Thus, there are promising reasons to promote research into the effects of vitamin D supplementation in COVID-19 patients. However, no studies to date have found that vitamin D affects post-COVID-19 symptoms or biomarkers. Based on this scenario, this review aims to provide an up-to-date overview of the potential role of vitamin D in long COVID-19 and of the current literature on this topic.
β-Lactam Antibiotics and β-Lactamase Enzymes Inhibitors, Part 2: Our Limited Resources
Authors: Alfei Silvana , Schito Anna Maria
Published: April 2022

β-lactam antibiotics (BLAs) are crucial molecules among antibacterial drugs, but the increasing emergence of resistance to them, developed by bacteria producing β-lactamase enzymes (BLEs), is becoming one of the major warnings to the global public health. Since only a small number of novel antibiotics are in development, a current clinical approach to limit this phenomenon consists of administering proper combinations of β-lactam antibiotics (BLAs) and β-lactamase inhibitors (BLEsIs). Unfortunately, while few clinically approved BLEsIs are capable of inhibiting most class-A and -C serine β-lactamases (SBLEs) and some carbapenemases of class D, they are unable to inhibit most part of the carbapenem hydrolyzing enzymes of class D and the worrying metallo-β-lactamases (MBLEs) of class B. Particularly, MBLEs are a set of enzymes that catalyzes the hydrolysis of a broad range of BLAs by a zinc-mediated mechanism, and currently no clinically available molecule capable of inhibiting MBLEs exists. Additionally, new types of alarming “superbugs”, were found to produce the New Delhi metallo-β-lactamases (NDMs) encoded by increasing variants of a plasmid-mediated gene capable of rapidly spreading among bacteria of the same species and even among different species. Particularly, NDM-1 possesses a flexible hydrolysis mechanism that inactivates all BLAs, except for aztreonam. The present review provides first an overview of existing BLAs and the most clinically relevant BLEs detected so far. Then, the BLEsIs and their most common associations with BLAs already clinically applied and those still in development are reviewed.