bluuebunny/crossref_metadata_2025 huggingface dataset:
Strategic Management for Sustainability
Authors: Negrão Keila Regina Mota , Gomes Sérgio Castro , Carvalho Mario Cesar dos Santos de , Emmendoerfer Magnus Luiz
Published: January 2025

ABSTRACT Objective: this study proposes a framework that integrates organizational resources and capabilities with sustainability dimensions to support the development of sustainable strategies. Grounded in the natural resource-based view (NRBV), this framework combines sustainability dimensions with organizational strengths to enhance value creation. Method: using a qualitative, theoretical, and exploratory approach, this study employs bibliographic research to conduct a conceptual analysis centered on strategic management for sustainability. Thesis: organizations achieve superior results when the value generated through resources and capabilities, aligned with sustainability dimensions, leads to value appropriation by consumers. Conclusions: the proposed framework begins by guiding organizations to identify their existing resources and capabilities. Following this, it encourages managers to explore ways to integrate sustainability into strategies that leverage these internal assets. Additionally, the framework prompts organizations to consider how their offerings (goods or services) can generate value for the enterprise while fostering value appropriation by customers. This value creation is viewed through three key aspects: a strong value proposition from economic and financial perspectives, the well-being provided to customers, and the alignment with the organization’s sustainable mission. The framework is designed to be flexible and adaptable, enabling its application across diverse organizational types and allowing for adjustments based on regional contexts. Moreover, it calls for future empirical studies to test, replicate, validate, or refine the framework, contributing to its practical relevance and potential evolution.
Gestão Estratégica para Sustentabilidade
Authors: Negrão Keila Regina Mota , Gomes Sérgio Castro , Carvalho Mario Cesar dos Santos de , Emmendoerfer Magnus Luiz
Published: January 2025

RESUMO Objetivo: propor um framework que integra os conceitos de recursos e capacidades organizacionais às dimensões da sustentabilidade, apresentando combinações que contribuam para a formulação de estratégias sustentáveis. A orientação epistemológica se alinha com as perspectivas da visão baseada em recursos naturais (VBRN) integradas às dimensões da sustentabilidade para criação de valor. A abordagem metodológica é qualitativa, teórica, exploratória e bibliográfica, configurando um ensaio com análise conceitual centrada na gestão estratégica para sustentabilidade. Tese: empreendimentos alcançam melhores resultados quando o valor criado por meio da combinação de recursos e capacidades associados às dimensões da sustentabilidade resulta em apropriação de valor pelos consumidores. Conclusões: se, por um lado, o framework proposto sugere a identificação dos recursos e capacidades que a organização possui, por outro, requer pensar em como gerar uma entrega (bens ou serviços) que possa criar valor para o empreendimento e estimular apropriação desse valor pelos clientes, considerando três aspectos: proposta de valor nas perspectivas econômica e financeira; bem-estar proporcionado aos clientes; e cumprimento da missão sustentável da organização da empresa. O framework apresentado indica flexibilidade e adaptabilidade, permitindo sua aplicação em diferentes tipos de organizações, assim como ressignificações e incrementos que podem variar de acordo com o território de atuação, além de provocar novos estudos empíricos para testar, replicar, validar ou contestar a proposta expressa neste ensaio. Trata-se de um modelo flexível, adaptável ao contexto em que se deseja estudar recursos e capacidades, sustentabilidade e criação de valor.
First report of hypoplastic left heart syndrome in 3p- syndrome and review of candidate genes
Authors: Böttcher Ana Kalise , Siqueira Monique Banik , Malgarezi Natasha , Nunes Marcela Rodrigues , Mergener Rafaella , Kalil Luisa Pigatto , Trevisan Patrícia , Zen Paulo Ricardo Gazzola
Published: January 2025

ABSTRACT Objective: 3p deletion syndrome is a rare monosomal disease that encompasses deletions throughout the short arm of chromosome 3. It is often in the distal region (3p25-pter), but variations in breakpoints and a complex clinical manifestation exist, with congenital heart defects being considered rare. We present the first case of hypoplastic left heart syndrome and minor dysmorphic features associated with 3p- syndrome. Furthermore, we aim to establish a gene-phenotype association. Case description: The diagnosis was made by karyotyping, followed by a literature investigation and in silico bioinformatic analysis about the possible candidate genes associated with congenital heart defects or hypoplastic left heart syndrome in 3p- syndrome. All genes analyzed that could affect heart formation are located in the 3p25.3 region, adjacent to the deleted region in the newborn from our case (3p26). Taking into account the technical limitations of the karyotype and the strength of evidence from each gene evaluated and locus proximity, it is likely that an unidentified partial break in the CAV3 gene occurred. Comments: We identified an indirect relation between gene CAV3 and hypoplastic left heart syndrome due to its strong association with cardiomyopathies and isolated cardiac defects. Furthermore, the cytogenetic band from our case is new information for the delimitation of a critical cardiac region on 3p syndrome, a discussion that has been ongoing since 1986. Thus, we reinforce the importance of cytogenetic investigation in patients with hypoplastic hearts and dysmorphia, assisting in diagnosis, definition of prognosis, and genetic counseling for the family.
The protective effect of zinc oxide nanoparticles on boar sperm during preservation at 17 °C
Authors: Hu Qimeng
Published: January 2025

Abstract More than 90% of spermatozoa of boars in pork producing countries is stored in liquid at 17 °C; however, the quality of these spermatozoa is affected by bacterial breeding and oxidative damage. This study analyzed sperm quality and sperm capacitation after storage to study the effects of the effects of ZnO nanoparticles (ZnO NPs) supplementation on seminal plasma (SP)-free sperm preservation. We investigated the effects of adding 20, 50, 100 and 200 μg/mL of ZnO NPs to a seminal free boar sperm diluent over a 7-day period at 17 °C to assess the changes in non-capacitated/capacitated sperm quality parameters, antioxidant capacity, ATP content and extent of protein tyrosine phosphorylation. The addition of different doses of ZnO NPs to stored sperm did not induce significant effects on the sperm motility and ATP content when compared to the sperm without ZnO NPs treatment. However, the addition of 50, 100, 200 μg/mL ZnO NPs to stored sperm improved total antioxidant capacity (T-AOC) and CuZn-superoxide dismutase (CuZn-SOD) (p < 0.05). ZnO NPs also reduced the malondialdehyde (MDA) content of the preserved sperm (p < 0.05). Moreover, our results indicate that the supplementation of 50 μg/mL ZnO NPs to preserved sperm improved the sperm membrane integrity (p < 0.05). ZnO NPs exerted protective effects on protein tyrosine phosphorylation, especially with regards to membrane proteins. Following incubation and capacitation, sperm exhibited good levels of protein tyrosine phosphorylation and ATP levels with high T-AOC and CuZn-SOD activity and low MDA content. ZnO NPs exerted protective capacity to a preservation extender used for SP-free boar sperm during storage at 17 °C. The optimal concentration of ZnO NPs for preservation extender was 50 μg/mL.
A simple and rapid evaluation of boar sperm quality using the resazurin colorimetric assay
Authors: Chu Fung-Hsiang , Chen Yu-Hsin , Kang Ting-Chieh , Chen Lih-Ren , Lin Hsiu-Lien Herbie
Published: January 2025

Abstract Ensuring boar sperm quality before insemination is crucial for maximizing field fertility and efficient pig production. The computer-assisted sperm analysis (CASA) and fluorescence probes combined with flow cytometry (FC) are commonly used techniques for evaluating sperm kinematics and functions, closely related to animal fertility. However, their high cost and complex operations make it challenging to apply them in laboratories or pig breeding farms with limited resources. Here, our aim was to develop a new protocol using a resazurin redox dye to assess boar sperm quality for practical application. We first created simulated semen samples with different levels of sperm quality (0, 20, 40, 60, 80, 100%) at concentrations of 300 and 150 × 106 cells/mL. Subsequently, the simulated semen was used to establish an optimal standard protocol based on the results of the resazurin colorimetric assay. Finally, the condition that showed the strongest correlation between resazurin redox rate and sperm parameters was selected to perform a linear regression test. Two optimal working conditions were identified, involved incubating 10 µL of resazurin reagent with 100 µL of semen for either 20 or 40 min, depending on the sperm concentration of either 300 or 150 × 106 cells/mL. We subsequently conducted a linear regression analysis using data that included the resazurin reaction rate and measurements of sperm parameters. Finally, we obtained two sets of five equations, allowing directly convert the absorbance of the resazurin assay into values for sperm quality parameters. These parameters include total motility, progressive motility, viability, acrosome integrity, and mitochondrial activity. This new protocol is valuable for sperm evaluation because it is cost-effective, time-efficient, and labor-saving.
Reactive oxygen species attenuation improves the quality of vitrified-warmed bovine embryos
Authors: Feuchard Viviane Luzia da Silva , Oliveira Clara Slade , Saraiva Naiara Zoccal , Quintão Carolina Capobiango Romano , Oliveira Leticia Zoccolaro
Published: January 2025

Abstract The aim of this study was to investigate the effects of modulating reactive oxygen species (ROS) in vitrified bovine in vitro produced (IVP) embryos. In experiment I we compared ROS production in fresh and vitrified-warmed blastocysts. In experiment II we evaluated the effects of antioxidant supplementation (100 μM of 2-mercaptoethanol; BME; 0 h to 2 h during warming) on ROS levels in vitrified-warmed blastocysts, and in experiment III we compared the development of fresh and vitrified-warmed blastocysts in the presence (BME) or absence (Control) of antioxidant (100 μM BME; 0 h to 48 h during warming). Higher ROS production (Fresh: 68.48 ± 7.92 vs Vitrified: 123.53 ± 13.15; P<0.05) and lower cell number was observed in vitrified compared to fresh embryos (Fresh: 123.01 ± 5.67 vs Vitrified: 103.04 ± 4.25; P<0.05). Antioxidant supplementation reduced ROS levels (Vitrified: 38.24 ± 1.27 vs. Vitrified/BME: 33.54 ± 1.08; P<0.05) and increased cell number in treated embryos (Vitrified: 100.65 ± 3.98 vs. Vitrified/BME: 112.95 ± 3.72; P<0.05). No differences were observed in the re-expansion rates of vitrified embryos cultured in the absence and presence of BME at 0, 2, and 4 h after warming (P>0.05). The embryo hatching rate did not differ (P>0.05) among embryos from the fresh, vitrified and vitrified/BME groups. However, the total cell numbers were higher (P<0.05) in vitrified embryos supplemented with BME (143.02 ± 6.97) than in vitrified embryos without BME (113.25 ± 5.09) but similar (P>0.05) to that observed in fresh embryos cultured with (150.54 ± 8.99) and without BME (142.71 ± 13.60). It was concluded that the vitrification and warming processes increased ROS levels in blastocysts and its attenuation with BME antioxidant improved embryonic quality.
Absence of mating behaviors in the female dogs neonatally treated with estrogen and progesterone
Authors: Park ChanJin , Tando Kayla , Soto-Heras Sandra , Zhou Sherry , Lin Po-Ching , Ko CheMyong
Published: January 2025

Abstract This study aimed to develop a non-surgical method to neutralize reproduction in female dogs. Female Beagle puppies, aged 6 days, were treated with pellets designed to release estradiol benzoate (EB; 1.0 mg) and progesterone (P4; 5.0 mg) over approximately 3 weeks. Their estrous cycles were monitored from 6 to 34 months of age by examining their vulvas daily and measuring their serum P4 levels once a month. Vulvar edema and discharge, followed by a serum P4 level above 5 ng/ml, indicated the potential estrus. Each time a dog showed these signs, breeding was attempted by housing with a proven male Beagle. All the treated dogs displayed cyclic progesterone surges with 5 to 6-month-long anestrous intervals. Surprisingly, none exhibited sexual behaviors, and no mating occurred (i.e., no intromission and copulatory tie), resulting in no pups being born. This phenomenon was further explored in laboratory animals. Neonatal female rats were treated with microspheres containing smaller doses of the same steroids (0.3 mg EB + 3.0 mg P4) at 1 or 2 days old. At 3 months old, the rats were ovariectomized, chemically stimulated to exhibit estrus behaviors using a standard protocol and tested for receptivity to proven male rats. Untreated control rats showed normal receptivity (i.e., lordosis) and allowed males to mate. However, rats treated with EB+P4 did not exhibit lordosis or allow mating. These results indicate that the combined use of estrogen and progesterone could be an effective non-surgical method for inhibiting mating behavior and, consequently, neutralizing female dog reproduction.
Tactile, thermal and gustatory stimulation therapy in the treatment of post-stroke oropharyngeal dysphagia: a scoping review
Authors: Matos Julia , Andrade Rodrigo Alves de , Bilheri Diego Fernando Dornelles , Santos Maysa Andrade , Oliveira Diane de Lima , Pernambuco Leandro de Araújo , Furkim Ana Maria
Published: January 2025

ABSTRACT Purpose To map in the literature the effects of tactile, thermal and/or gustatory stimulation on oropharyngeal dysphagia (OD) post-stroke. Methods This scoping review was conducted following the recommendations of PRISMA- ScR and the Joanna Briggs Institute (JBI), registered on the Open Science Framework and developed without language or publication period restrictions. Different databases and grey literature were used for article selection, and the PCC mnemonics constructed the research question ad eligibility criteria, thus including clinical studies involving adults (over 18 years old) diagnosed with OD post-stroke, who received tactile-thermal (TTS) and/or taste-gustatory (TGS) and/or tactile-thermal-gustatory stimulation for treatment, and had their effect measured through examinations, scales, or clinical assessment. The review was conducted blindly and independently by two researchers, and a third was consulted in cases of disagreements. Results Three studies were included. None of them addressed an individual TGS protocol, and all presented a TTS protocol. The objectives and evaluation tests of each study were distinct, providing different perspectives about TGS in OD; there was uniformity in the presentation of the applied protocols, frequency, and materials used for therapy. Conclusion The studies mapped the effect of TTS from different methodological designs and specific measures; no articles were found that evaluated isolated TGS associated with another technique.
Terapia de estimulação tátil, térmica e gustativa no tratamento da disfagia orofaríngea pós AVCi: uma revisão de escopo
Authors: Matos Julia , Andrade Rodrigo Alves de , Bilheri Diego Fernando Dornelles , Santos Maysa Andrade , Oliveira Diane de Lima , Pernambuco Leandro de Araújo , Furkim Ana Maria
Published: January 2025

RESUMO Objetivo Mapear na literatura os efeitos da estimulação tátil, térmica e/ou gustativa na disfagia orofaríngea (DO) pós AVCi. Método Esta revisão de escopo foi conduzida segundo recomendações do PRISMA-SCR e do Joanna Briggs Institute (JBI). Registrada na Open Science Framework e desenvolvida sem restrições de idioma ou período de publicação. Foram utilizadas diferentes bases de dados e literatura cinzenta para seleção dos artigos, e os mnemônicos PCC construíram a pergunta e critérios de elegibilidade da pesquisa, assim, foram incluídos estudos clínicos envolvendo adultos (maiores de 18 anos) diagnosticados com DO pós AVCi, que receberam estimulação tátil-térmica (ETT) e/ou térmica-gustativa (ETG) e/ou estimulação tátil-térmica-gustativa para tratamento da DO e tiveram seu efeito medido a partir de exames, escalas ou avaliação clínica. A revisão foi conduzida de forma cega e independente por dois pesquisadores, e um terceiro foi consultado em casos de divergências. Resultados Três estudos foram incluídos. Nenhum deles abordou um protocolo individual de ETG, e todos apresentaram protocolo de ETT. Os objetivos e os testes de avaliação de cada estudo foram distintos, apresentando diversas perspectivas acerca da ETT na DO; houve uniformidade na apresentação dos protocolos aplicados, frequência e materiais utilizados para a terapia. Conclusão Os estudos mapearam o efeito da ETT a partir de diferentes desenhos metodológicos e medidas específicas; não foram encontrados artigos que avaliassem ETG isolada ou associada à outra técnica.
Digital platforms: the contemporary picture of exploration at work and the challenges to workers’ health
Authors: Salvagni Julice , Veronese Marilia Veríssimo , Figaro Roseli
Published: January 2025

Abstract Objective To identify studies about working in the context of the platform economy, regarding the consequences of algorithmic management, financialization and datafication for workers´ health. Methods Integrative review of occupational health literature published in Brazilian journals between 2017 and 2022. Searches were conducted on the Capes Journal Portal and SciELO. Results Of the 324 articles identified in the survey, 10 met the established criteria. The selected studies showed that in platform work, asymmetrical production relations are concealed, which can accentuate control and exploitation. We identified a management logic that seeks to simulate relationships between “partners”, exempting itself from the rights associated with decent work. As a result, workers noticed an increase in feelings of subsumption, insecurity and loss of meaning in their work, elements that are harmful to their psychosocial health. Conclusion Algorithmic management and job insecurity cause harm to physical and mental health, making more research necessary to quantify and describe with greater precision the difficulties and coping strategies of the workers, so that it can contribute to the development of policies that regulate the sector.
Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds
Authors: Ferreira Elisângela Elduina , Ferreira Renan Diniz , Almeida Milena Santos de , Costa Renan Araújo , Silva Izabela Caputo Assis , Morais Melissa Grazielle , Bertolin Karen Helaine Mendes , Vidigal Paula Vieira Teixeira , Costa Raquel Alves , Lima Luciana Alves Rodrigues dos Santos , Pinto Flávia Carmo Horta
Published: January 2025

ABSTRACT Purpose: To evaluate the effect of the topical application of the ethanol extract (EESL) and the hydroethanolic fraction (HFSL) of ripe Solanum lycocarpum fruit on the healing of experimentally-induced wounds in mice. Methods: The EESL and HFSL obtained from ripe fruit of the species S. lycocarpum were obtained by percolation with ethanol. They were tested in the healing of excisional wounds in mice, which were induced in anesthetized animals using a 7-mm dermatological punch. They were divided according to the treatment period and group, n = 6, and received topical application of the EESL or HFSL daily or saline solution for one, five, seven, or 21 days. At the end of this period, the animals were euthanized, and the lesions were subjected to histopathological processing and analysis to evaluate macroscopic area of the wound and microscopic analysis by morphometry of the number of leukocytes, mast cells, fibroblasts, re-epithelialization, and matrix deposition. Results: The application of the EESL and HFSL reduced the number of leukocytes after one, five, and seven days of treatment. EESL improved re-epithelialization and tissue proliferation in wound healing from day 7. Conclusion: The study demonstrated that daily administration of EESL and HFSL exhibit wound healing activity, with this effect being more pronounced by EESL. To our knowledge, this is the first report of the anti-inflammatory and healing activity of this species through topical application.
GROWTH TRAJECTORY AND INFORMAL SECTOR BUSINESSES IN NIGERIA: THE CASE OF ENUGU STATE
Authors: Uzoechina Benedict Ikemefuna , Okeke Ambrose Chiedozie , Ekwoh Geraldine Amaka , Oladipo Abimbola O.
Published: January 2025

ABSTRACT The informal sector has become the bedrock for employment creation. However, its capacity for growth and production of goods and services remains doubtful. This study investigated the growth path of businesses in the informal sector and factors that sustain this growth trajectory in Enugu State, Nigeria. A sample of 500 respondents was chosen from two communities in each of the two local government areas from the three senatorial zones within Enugu State. The sampling techniques employed were purposive and stratified random sampling. Survey and quantitative research techniques were adopted. Results did not confirm a vertical growth trajectory of informal sector businesses but confirmed that economic, demographic, and environmental factors drive the informal sector horizontal growth trajectory in Enugu State. Therefore, we recommend a paradigm shift that curbs corruption, allows the gains from growth to trickle down, creates access to finance, and provides critical infrastructure to facilitate the vertical integration of informal sector businesses with the formal sector for improved productive capacity.
HOW STATE-OWNED SHAREHOLDERS UNDER GOVERNMENT INTERVENTION AFFECT THE RE-INNOVATION DECISION OF ENTERPRISES AFTER TECHNOLOGICAL INNOVATION FAILURE?
Authors: Yang Zhang , Wentao Zhou , Bingwei Jiang
Published: January 2025

ABSTRACT In a mixed ownership reform context, the participation of state-owned capital in private enterprises affects re-innovation decisions after the failure of technological innovation. This article takes a sample of A-share pharmaceutical manufacturing listed companies in China to test the impact of state-owned shareholders and their shareholding ratios on the re-innovation decisions after these enterprises have experienced technological innovation failure. The research found that state-owned shareholders in these enterprises are more likely to reduce additional innovation and adopt new innovations after the experience of failing to implement technological innovation. This phenomenon is more obvious with the increased proportion of state-owned shareholders. In addition, government intervention can effectively suppress the positive impact of state-owned shareholders on new innovation in enterprises that have experienced technological innovation failure. This paper aims to provide a theoretical basis and decision-making reference for private enterprises to improve their governance structure by introducing state-owned capital shares and relevant government departments to stimulate and optimize their re-innovation decision after technological innovation failure through appropriate intervention in private enterprises’ technological innovation.
COMO OS ACIONISTAS ESTATAIS SOB A INTERVENÇÃO DO GOVERNO AFETAM A DECISÃO DE INOVAR NOVAMENTE NAS EMPRESAS APÓS FALHA DE INOVAÇÃO TECNOLÓGICA?
Authors: Yang Zhang , Wentao Zhou , Bingwei Jiang
Published: January 2025

RESUMO No contexto da reforma da propriedade mista na China, a participação do capital estatal em empresas privadas afeta a decisão de inovar novamente após a empresa já ter falhado em implementar inovação tecnológica no passado. Este artigo toma as empresas listadas de fabricação farmacêutica de ações A na China como amostras de pesquisa para testar o impacto dos acionistas estatais e suas taxas de participação na decisão de uma nova inovação de empresas privadas após o fracasso da inovação tecnológica. A pesquisa descobriu que os acionistas estatais são mais propensos a reduzir a inovação adicional e adotar a nova inovação após o fracasso. Esse fenômeno será mais evidente com o aumento da proporção de acionistas estatais. Além disso, a intervenção governamental pode efetivamente suprimir o impacto positivo dos acionistas estatais sobre a nova inovação após o fracasso das empresas privadas. Este trabalho tem como objetivo fornecer base teórica e referência de tomada de decisão para que as empresas privadas melhorem sua estrutura de governança por meio da introdução de ações de capital estatais, e departamentos governamentais relevantes para estimular e otimizar sua decisão de inovar novamente após falha de inovação tecnológica por meio de intervenção adequada na inovação tecnológica das empresas privadas.
Acidity index of different buriti oil samples subjected to spectroscopy in infrared region and principal component analysis
Authors: Silva Braian Saimon Frota da , Batista Bruno Willams Maciel , Costa Kevin Lucas Soares , Alves Cláudio Nahum , Pereira Simone de Fátima Pinheiro , Ferreira Nelson Rosa
Published: January 2025

Abstract The objective of this work was to evaluate the use of chemometric tools associated with mid-infrared waves (ATR-MIR-FTIR) to determine the acidity index (AI) of Mauritia flexuosa oil extracted from plants from different locations. Thirty oil samples were collected in five municipalities of the state of Pará, Brazil. The principal component analysis, combined with Hotelling’s T² multivariate control test, was used to identify anomalous samples from the spectra. The ATR-MIR-FTIR analysis showed that the oil contains mainly monounsaturated acids, including oleic acid, which is directly correlated with AI. The first two principal components explained 99.93% of the cumulative variance. The statistical methods identified patterns of similarities between the samples with a close AI and tested an outlier from the sample with the highest AI. The application of ATR-MIR-FTIR, associated with chemometric methods, is appropriate to evaluate the acidity of buriti palm oil samples.
Fermentative characteristics, agronomic evaluation, and microbial populations of sorghum silage with additives
Authors: Venturini Tiago , Neres Marcela Abbado , Tinini Rodrigo Cesar dos Reis , Dessbessell Jéssica Gabi , Schneider Pâmela Rosana , Schneider Cibele Regina , Muxfeldt Luana , Baungratz Andressa Radtke , Fischer Maria Luiza , Zambom Maximiliane Alavarse
Published: January 2025

Abstract The objective of this work was to evaluate the effects of the use of additives on the ensiling of AGRI 002E sorghum (Sorghum bicolor), focusing on the chemical composition, loss, microbial profile, and fermentative stability of the fodder. Sorghum was harvested 111 days after sowing, chopped, and ensiled in PVC silos under different treatments, as follows: without additives (SS), with inoculant (SSI), with urea (SSU), and with urea and inoculant (SSUI). The silos were opened and analyzed at 1, 3, 7, 14, 28, and 56 days after ensiling. The inclusion of urea and bacterial inoculant did not significantly affect dry matter content, but influenced dry matter recovery and microbial population, reducing the presence of clostridia and fungi. Dry matter and gas losses were minimal, indicating an adequate fermentation. The additives contribute to a more stable fermentation and a better preservation of the ensiled material, particularly by reducing undesirable microbial populations.
Artemisia dracunculus essential oil: phytochemical study, pre-treatment and co-treatment effects on morphine withdrawal syndrome
Authors: Lorigooini Zahra , Heibati Fatemeh , Ahmadi Seyyed Matin , Mohammadi Sareh , Rafieian-Kopaei Mahmoud
Published: January 2025

Abstract Drug addiction is one of the most important global problems. Medicinal herbs have been traditionally used in the management and treatment of opioid withdrawal syndrome and pain. The aim of this study was to determine the effect of A. dracunculus essential oil in reducing the symptoms of morphine withdrawal in mice. Male mice (25-30 g) were randomly assigned into 4 groups (n=10). Morphine-dependent groups received morphine (50 and 75 mg/kg ip; three times/day, 3 days) and a single injection of morphine (50 mg/kg) and then naloxone on the fourth day via IP injection. The control group received saline. The post-treated group received morphine for 3 days; on the fourth day, ten minutes before receiving naloxone, A. dracunculus essential oil with a dose of 75 mg/kg was injected. All groups received naloxone 2 hours after receiving the last dose of morphine; then, the morphine withdrawal symptoms were measured for 30 minutes. In the post-treated and co-treated groups, the body stretching and shaking the claw were significantly less than morphine-dependent groups (p<0/05). Also, in the post-treated and co-treated groups, the blinking, itching, and the number of standing on two legs significantly decreased compared to the morphine group. Hence, it might be concluded that A. dracunculus essential oil can significantly reduce morphine withdrawal symptoms.
Evaluation of operational parameters for clinical evaluation of skin hydration by corneometry method
Authors: Aiello Laura Moretti , Vergílio Mariane Massufero , Silva Silas Arandas Monteiro e , Leonardi Gislaine Ricci
Published: January 2025

Abstract The Corneometer CM® 825 from Courage & Khazaka electronic GmbH is widely utilized in clinical efficacy testing of cosmetics. Assessing methodological variables is crucial for ensuring the reliability of the process. The aim of this study was to evaluate the operational and instrumental parameters of the Corneometer CM® 825 in the clinical assessment of skin hydration levels. Precision and accuracy of the measurements were assessed by measuring hydration levels on 10 participants in specific areas of the forearm and face. Three different skin conditions (dry, normal, and hydrated) resulting from the use or non-use of moisturizing cosmetics and a drying promoter were evaluated within 120 minutes of their application. The electrical capacitance method demonstrated good repeatability, with coefficients of variation mostly below 10%. However, higher variability was observed in inter-observer readings. The equipment exhibited high sensitivity and accurately discriminated the differences between hydrated and dry skin. Operational training and standardization of the methodology are essential to ensure accurate, reproducible, and reliable results in studies utilizing the Corneometer equipment for assessing skin hydration levels.
L-theanine modulates tissue cortisol levels in zebrafish after single stress
Authors: Silva Vinícius Luís da , Mayer Mariana Spanamberg , Severo Andriele dos Reis , Zambra Andressa Leal , Almeida Joanna Moraes De , Sestari Laura Moura , Parisi Mariana Migliorini , Giacomini Ana Cristina Vendrametto Varrone , Abreu Murilo Sander De , Bortolotto Josiane Woutheres , Bonfanti-Azzolin Gabriela
Published: January 2025

Abstract L-theanine has neuromodulatory properties, such as attenuate anxiety disorders and stress. Stress is a situation characterized by the stimulus to cortisol secretion and without specific pharmacological therapies. Thus, this study investigates the effect of L-theanine on the response to acute stress, evaluated by tissue cortisol level, oxidative status markers, and behavioral parameters, in zebrafish. The animals were divided into six groups: G1: control, G2: treated with L-theanine 45 mg/L, G3: treated with L-theanine 100 mg/L, G4: stress, G5: stress+L-theanine 45 mg/L and G6: stress+L-theanine 100 mg/L. Before the execution of the acute stress protocol that consisted of chasing the animals with a net for two minutes, exposure to L-theanine was performed in aquarium water for 1 hour. Tissue cortisol levels were analyzed, as well as cerebral oxidative status (lipoperoxidation and levels of thiolic compounds) and fish behavior (new tank test). L-theanine at a concentration of 45 mg/mL modulated the effects of acute stress on zebrafish, inhibiting increased levels of tissue cortisol. However, it was not able to alter the behavior or oxidative status of the animals. Thus, we conclude that the administration of L-theanine can promote acute stress attenuation, modulating the pathological responses of stress.
Entecavir: stability and drug-excipient compatibility
Authors: Fiorot Ariadne Botto , Santos Clayton Raynan dos , Xavier Thiago Padovani , Lira Taisa Shimosakai de , Oliveira Marcelo Antonio de
Published: January 2025

Abstract Entecavir is an inhibitor of hepatitis B virus (HBV) DNA synthesis that has been widely prescribed in the treatment of chronic infections caused by the virus. Production of generic ETV drugs is an ongoing global endeavor, with particular significance for developing countries that rely on importing the expensive reference drug. ETV-excipient compatibility studies were conducted with the declared inputs in solid pharmaceutical formulations on the market through thermal analysis and HPLC techniques. Thermal analyses by TGA and DTA indicated compatibility of entecavir with the excipients microcrystalline cellulose, crospovidone, titanium dioxide, magnesium stearate, hypromellose, polyethylene glycol and povidone; this was confirmed via HPLC. Lactose monohydrate proved to be incompatible with ETV in thermal and chromatographic assays. The thermal analysis of marketed solid dosage forms revealed a predominance of the lactose monohydrate profile at the expense of ETV and other inputs in the TGA and DTA curves, due to its high content in the formulations; this makes the evidence of ETV-lactose chemical interaction even more important. Compatibility tests by HPLC showed no chemical interaction of ETV with the excipient mannitol, a soluble diluent proposed as a replacement for lactose monohydrate, with the same function in the formulation.
Analysis of the effectiveness of premedication in preventing early adverse reactions to antivenom serum from 2017 to 2021: A cohort study
Authors: Priedols Gustavo Abud , Alves Jonas Alher Meira , Oliveira Jordana Meirelles de , Rufino Jéssica Vertuan , Girotto Edmarlon , Guidoni Camilo Molino
Published: January 2025

Abstract This study aimed to analyze the effectiveness of using an antivenom serum (AVS) premedication protocol to prevent early adverse reactions (EAR). This is a cohort study conducted with data from the Londrina Information and Toxicological Assistance Center (CIATox-Londrina) at the University Hospital of the State University of Londrina (HU-UEL), which included patients treated with AVS between January/2017 and December/2021. The independent variable was performing the premedication protocol and the dependent variable was the occurrence of EAR after AVS administration. Patients were followed until hospital discharge. The association analysis was performed with calculation of the Relative Risk (RR) and 95% Confidence Interval (95% CI). A total of 806 cases of accidents with venomous animals were analyzed. The minority (8.1%; n=65) had EAR, and the most frequent symptoms were related to dermatological and respiratory manifestations. The AVS premedication protocol was performed in 96.6% (n=734) of the cases. There was no statistically significant difference in the unadjusted (RR: 1.487; 95% CI: 0.530-4.174) and adjusted (RR: 1.482; 95% CI: 0.522-4.210) models regarding the performance of the AVS premedication protocol and the occurrence of EAR. It is recommended against AVS premedication given the lack of effectiveness in preventing EAR.
Synthesis, characterization, antimicrobial, antioxidant, and anti-cancer activity of new hybrid structures based on benzimidazole and thiadiazole
Authors: Bostancı Hayrani Eren , Çevik Ulviye Acar , Işık Ayşen , Maryam Zahra , İnce Ufuk , Özkay Yusuf , Kaplancıklı Zafer Asım
Published: January 2025

Abstract Hybrid structures containing multiple pharmacophore units with known activity have attracted attention due to their promising outcomes. In this study, several new hybrid structures containing benzimidazole and thiadiazole units were synthesized. The newly synthesized compounds were structurally analyzed using 1H-NMR, 13C-NMR, HRMS, and elemental analysis. The antimicrobial, in vitro anti-cancer, and antioxidant activities of all compounds were investigated. In vitro antimicrobial activities of the compounds were determined against Gram-positive (S. aureus ATCC 29213, E. faecalis ATCC 29212), Gram-negative (E. coli ATCC 25922, P. aeruginosa ATCC 27853) bacteria and fungi (C. albicans ATCC 10231) by using broth microdilution method. The compound 5g bearing 4-methoxyphenyl derivative showed the best activity with 32 μg/mL against S. aureus ATCC 29213 and P. aeruginosa ATCC 27853. The MTT test was used to determine the cytotoxicity of the produced compounds on the MCF-7 (human breast cancer) and L-929 (fibroblast) cell lines. FRAP method was used to determine the antioxidant properties of synthesized compounds. The Ferric Reducing Antioxidant Power of the compounds 5a, 5b, and 5c showed more antioxidant properties than vitamin E. The compound 5g stands out in the series in that it is not toxic on the healthy cell line and has promising antimicrobial activity.
Hydromethanolic extracts of Morus alba L. (Mulberry) young and ripe fruit induces apoptosis in lung carcinoma cells (A549)
Authors: Sargara Payal , Nataraj M. , Subramanian Ramalingam B. , Thakkar Anjali B.
Published: January 2025

Abstract The study investigates the potential of Morus alba L. (mulberry) young and ripe fruit extracts against lung cancer cells. Cancer ranking as the second leading cause of global mortality, it is essential to investigate natural compounds like phytochemicals for therapeutic benefits. The research investigates presence of phytochemicals and antioxidant activity of both young (MAF-Y) and ripe (MAF-R) mulberry fruit extracts. Results revealed presence of various secondary metabolites, particularly high phenolic content and antioxidant properties in MAF-R. Both extracts demonstrated significant cytotoxicity against lung adenocarcinoma cells (A549), with IC50 18.4 ± 3.01µg/ml (MAF-R) and 29.41 ±3.6 µg/ml (MAF-Y). Moreover, the extracts effectively inhibited cell migration. Treatment of extracts elevated reactive oxygen species (ROS) production which resulted in disruption of mitochondrial membrane potential, and induced the process of apoptosis in lung carcinoma cells. This was evidenced through various assays including differential staining and DNA fragmentation analysis. These findings underscore the potential of mulberry fruit extracts as promising candidates for cancer prevention and treatment due to their antioxidant properties, cytotoxic effects, and ability to induce apoptosis in lung cancer cells.
The Effect of long-term rapamycin administration on intracellular calcium quantification and RhoA activity in kainic acid epilepsy model
Authors: Husna Machlusil , Handono Kusworini , Sujuti Hidayat , Aulanni’am Aulanni’am , Karimah Rumman , Waafi Afiyfah Kiysa , Satira Alya
Published: January 2025

Abstract Epilepsy affects around 50 million people worldwide, with 30% of them being refractory epilepsy. This shows that there is still a need for novel anti-seizure medication that have different mechanisms. One of the most common types of refractory epilepsy is temporal lobe epilepsy. Among the several effects of seizures on neurons is an increase in intracellular Ca2+ and activation of RhoA. Rapamycin is mTORC1 inhibitor, but long-term exposure to rapamycin (>18 hours) could also inhibit mTORC2. RhoA signaling pathway is regulated through the mTORC2 pathway; thus we hypothesized that long-term exposure to rapamycin could inhibit intracellular Ca2+ and RhoA activity as one of the mTORC2 downstream proteins, in a temporal lobe epilepsy model. This study used organotypic hippocampal slice cultures (OHSC) which were exposed to 20 nM rapamycin treatment for 3, 5, 8, and 10 days after induction of epilepsy by 7 μM kainic acid administration for 48 hours. Intracellular calcium concentration was observed using CLSM and RhoA activity with western blot. The results obtained from this research were long-term administration of rapamycin can decrease intracellular calcium concentration and RhoA activity in OHSC models of epilepsy induced by kainic acid, with the most effective duration is 5 days of exposure to rapamycin.
Rifampicin liposomes loaded on calcium phosphate cement scaffold constructed using 3D printing technology for sustained drug release
Authors: Jin Jie , Zheng Mingfeng , Cai Xinjun , Fan Xudong
Published: January 2025

Abstract Rifampicin (RFP) is used for the treatment of chronic bone tuberculosis owing to its powerful and wide spectrum antibacterial activities. However, effective concentrations of RFP required to treat bone tuberculosis are maintained for a short time in vivo, and adverse reactions and bone defects may occur after surgery. Therefore, the construction of a new drug delivery system to overcome these problems is required. In this study, we designed, constructed, and demonstrated the applicability of a calcium phosphate cement scaffold loaded with RFP liposomes for the treatment of bone tuberculosis. RFP liposomes were prepared using a film dispersion method. The preparation method was optimized using the encapsulation rate as an indicator and the morphology, mean particle size, zeta potential, encapsulation rate, and drug loading of RFP liposomes were characterized. Calcium phosphate cement scaffolds were constructed using 3D printing technology and used as RFP liposome carriers for sustained-release drug delivery. Finally, some of the properties were verified in vivo through experiments in rabbits. The results indicated that composite scaffolds can provide sustained drug release and are a promising treatment option for bone tuberculosis.
El swipe infinito: Efecto de las aplicaciones de citas en las relaciones románticas vis-à-vis
Authors: Chaux Alcides
Published: January 2025

Este artículo analiza el papel de las aplicaciones de citas en la configuración de las relaciones románticas, destacando los principales desafíos que surgen en la interacción digital. A pesar de que estas aplicaciones han democratizado el acceso a posibles parejas y ofrecen mayores oportunidades de encuentro, su uso intensivo acarrea obstáculos como la percepción de una oferta «infinita», la propensión a comportamientos evasivos («ghosting», «breadcrumbing») y la búsqueda de validación constante. Dichas dinámicas pueden derivar en relaciones marcadas por la inmediatez y la superficialidad, dificultando la maduración de la intimidad y el compromiso. Asimismo, la competitividad refleja la tensión entre la conveniencia de los contactos virtuales y la necesidad de cultivar valores fundamentales para vínculos sólidos, como la empatía y la honestidad. Con ello, se destaca la importancia de la responsabilidad digital tanto a nivel de los usuarios como de las plataformas, así como la relevancia de la claridad de objetivos y un enfoque consciente para equilibrar las ventajas tecnológicas con la esencia relacional y afectiva de los encuentros presenciales.
¿Puede la luz de color ayudar a mejorar el aprendizaje de la competencia lingüística escrita?
Authors: Quiles-Rodríguez José , Palau Ramon
Published: January 2025

Els colors dels entorns educatius influeixen en el procés d'aprenentatge dels alumnes. Tradicionalment això s'ha tingut en compte i s'ha estudiat, però sense la sistematicitat necessària com per a extreure conclusions pràctiques. No obstant això, la tecnologia actual, llum emesa pels díodes (LED), permet generar una font de "llum de color" el caràcter dinàmic de la qual permet adaptar-la a les necessitats de cada procés educatiu. En aquest estudi analitzem la influència d'aquesta "llum de color" en la competència lingüística escrita de l'alumnat de primària. Per a això, utilitzem un mètode quasiexperimental amb un grup de control basat en un disseny equivalent de materials, que va tenir lloc en una escola rural d'educació primària. Es van dissenyar quatre escenaris de "llum de colors", servint el primer d'ells com a pretest. Es van prendre valors repetits d'expressió i comprensió escrites, observant la seva variació segons l'exposició a diferents escenaris de "llum de color". Els resultats de l'estudi mostren una major significació en l'expressió que en la comprensió. El dinamisme de la tecnologia de la "llum de color" resulta interessant ser tingut en compte per a la seva aplicació a l'aula.
Critical Determinants of COVID-19 Severity and Predictive Modeling for Healthcare Optimization
Authors: Hammad Uallah , Rija Ali , Saad Ali , Umair Arif
Published: January 2025

The COVID-19 pandemic placed unprecedented strain on global healthcare systems, highlighting the need to identify critical determinants of disease severity and develop predictive models for resource optimization. This study aimed to identify the most significant factors influencing COVID-19 severity, analyze comorbidity patterns, and develop machine learning models for predicting severe outcomes. Using a dataset of 1,000 COVID-19 patients, demographic, clinical, and medical history data were analyzed. Comorbidities such as COPD (96.3%), chronic renal disease (92.6%), cardiovascular issues (93.9%), and diabetes (69.9%) were found to be highly prevalent among severe cases. Over half of the patients required ICU admission (51.1%) or ventilator support (54.5%), indicating the critical impact of severe COVID-19 symptoms on healthcare systems. Four machine learning models decision tree, logistic regression, random forest, and AdaBoost were evaluated for predictive accuracy using a 20-80 ratio and 10-fold cross-validation. In the 20-80 ratio, AdaBoost and logistic regression emerged as the most effective models, achieving 77.00% accuracy, with AdaBoost excelling in precision at 79.84% and specificity at 91.75%, and Logistic Regression providing the highest sensitivity at 67.96% for balanced predictions. The average results across all folds were as follows: Decision Tree accuracy was 65.80%, Random Forest accuracy was 72.40%, Logistic Regression accuracy was 75.40%, and AdaBoost accuracy was 75.50%. These findings underscore the importance of comorbidities in determining COVID-19 severity and demonstrate the utility of predictive modeling in optimizing healthcare resources. The study concludes that tailored interventions for high-risk patients and machine learning-driven resource allocation strategies can enhance healthcare efficiency during pandemics.
Spatial Layout Characteristics of Northern Wei Luoyang: A Spatial Humanities Analysis of the “Record of the Monasteries of Luoyang” v1
Authors: Sun Runfeng , Liu Wenjia
Published: January 2025

This paper explores the urban spatial structure and social stratification of Northern Wei Dynasty Luoyang City, based on Record of the Monasteries of Luoyang. Adopting a spatial humanities approach, the study integrates quantitative approaches, including the application of geographic information systems (GIS) for spatial analysis and complex network analysis. The findings indicate that the spatial organization of Northern Wei Luoyang was primarily concentrated within and around the inner city. People of different social classes and statuses occupied distinct spaces, forming socially stratified groups. As one moves outward from the city's center, the social rank of these groups decreases. The city's layout is characterized by a single central axis, with multiple spatial divisions, and the west side being associated with higher-status groups. This spatial arrangement was shaped by factors such as geography, urban development, Central Plains rituals, traditional customs, and ethnic diversity. This study enhances our understanding of the spatial layout characteristics of Northern Wei Luoyang. It provides innovative insights and methods for understanding the city’s structure and its implications for other underground historical cities. Furthermore, it provides valuable data and visualizations that could support future preservation and planning efforts for the city.