bluuebunny/crossref_metadata_2025 huggingface dataset:
P.133 Neurons in the lateral prefrontal cortex encode task features during virtual navigation
Authors: Abbass M , Corrigan B , Johnston R , Gulli R , Sachs A , Lau JC , Martinez-Trujillo J
Published: May 2024

Background: The lateral prefrontal cortex (LPFC) is uniquely found in primates and has been associated with contextual learning. This function is thought to be subserved by neurons that are tuned to abstract concepts and the combination of those concepts. LPFC neuron tuning remains to be fully investigated in naturalistic conditions. Methods: Two macaques were trained to perform a context-colour association task while using a joystick to navigate in an X-shaped maze. They were implanted with two 96-channel microelectrode arrays, targeting the LPFC. Mean firing rates were computed and multivariate linear regressions were used to determine tuning. Results: LPFC neurons were tuned to context (12.4%), color position (6.2%), target side (17.2%), and were selective to more than one feature (21.2%). LPFC neurons acquired tuning to task features in an ordered manner, starting with context (130.1±27.4ms), followed by the colour position (296.2±21.4ms) and then target side (493.3±19.3ms). Furthermore, most neurons (54%) changed their tuning over time. Conclusions: We demonstrate that single neurons can encode relevant features embedded in a naturalistic virtual environment. Our results support previous observations that LPFC neurons combine individual features and suggest that these features are also combined temporally. These findings contribute towards understanding the LPFC and have potential practical implications.
P.138 Burnout in Canadian neurosurgery
Authors: Skulsampaopol J , Shitsama S , Cusimano MD
Published: May 2024

Background: Burnout is common among health care professionals and can lead to depression and poor patient outcomes. The prevalence of burnout among Canadian neurosurgeons and trainees is yet unknown. Methods: International survey of neurosurgeons and trainees Results: Of total 403 responses, 47 were Canadian respondents (80.9% were male and 14.9% were female). Rate of burnout among Canadian neurosurgeons and trainees was 42.6%; however, there was no significant difference between rate of burnout between Canadian respondents and non-Canadian respondents (35.3%), p=0.33. Rate of burnout among Canadian neurosurgeons and resident/fellow was 40 and 47.1%, respectively, p=0.64. Subgroup analysis showed no difference in rate of burnout between Canadian and non-Canadian practicing neurosurgeons (p=0.34) and Canadian and non-Canadian resident/fellow (p=0.76). Canadian neurosurgeons with work experience of 5-10 years are more likely to have burnout compared to neurosurgeons with more or less work experience (OR 17, 95%CI 1.43-826.22, p=0.005). There was a trend that female Canadian respondents had more burnout than male counterparts (OR 4.2, 95%CI 0.57-47.45, p=0.09). Conclusions: Burnout is not uncommon among Canadian neurosurgeons/trainees. Monitor and supports should be provide to those who are at risk to mitigate burnout and provide resilience.
GR.2 Obesity and multiple sclerosis severity: a Mendelian randomization study
Authors: Alzamanan F , Ding Y , Harroud A
Published: May 2024

Background: Obesity is increasingly implicated in the development of multiple sclerosis (MS), but its effect on disease disability is less well-established. This study aims to investigate the association between obesity and MS severity utilizing Mendelian Randomization (MR). Methods: Employing a two-sample MR setting, we examined the effects of various obesity measures and adiposity distribution metrics on MS severity. Genetic proxies for body mass index (BMI) were selected from a study of 806,834 participants, with MS severity determined from a genetic study of age-related MS severity scores in 12,584 individuals with MS. Results: The main analysis reveals an association between elevated BMI and increased MS severity (P = 0.03). This is supported by a significant effect of whole body fat (P = 0.04), aligning with the hypothesis that obesity exacerbates MS disability. Sensitivity analyses suggest minimal heterogeneity and bias, indicating a potential causal effect. Conclusions: Our findings suggest that obesity adversely influences long-term disability outcomes in MS. The convergence of this genetic evidence with some of the prior observational studies strengthens the argument for a causal relationship between obesity and MS severity. These insights highlight obesity as a potentially modifiable risk factor in managing MS, underscoring the importance of weight management in MS treatment strategies.
C.2 An in-depth analysis of pediatric inflammatory myopathies: findings from a comprehensive tertiary care hospital
Authors: Mostofi E , McMillan H , Jurencak R , Hamilton L , Yaworski A
Published: May 2024

Background: Pediatric inflammatory myopathies (PIM) are a rare, heterogenous group of disorders requiring prompt diagnosis and treatment to reduce complications and improve long-term outcome. This study reviews the clinical characteristics, management, and outcomes in PIM. Methods: A retrospective analysis of pediatric patients diagnosed with PIM at CHEO from January 2009 to December 2023 was performed. Patient data, including age at symptom onset, diagnostic testing performed, treatment, and follow-up durations, were evaluated. Results: A total of 25 patients with juvenile dermatomyositis (JDM), overlap syndromes, and necrotizing myopathy (HMG-CoA reductase and anti-SRP myositis) were identified. Symptoms began at an average age of 8.37 years (1.10-14.11), with formal diagnosis occurring at 8.57 years (2.02-16.11). Initial symptoms included skin changes, muscle weakness, joint pain, and fatigue. Diagnosis involved laboratory testing (CK, myositis antibodies), muscle MRI, electromyography, and/or muscle biopsy. Treatments included corticosteroids, IVIG, and steroid-sparing agents (methotrexate, mycophenolate mofetil, rituximab, hydroxychloroquine). Follow-up averaged 4.23 years (range: 0.5 to 13). Most patients displayed only mild residual symptoms with the exception of an anti-SRP myositis patient who became wheelchair-dependent, requiring ventilatory support. Conclusions: Inflammatory myopathies require prompt treatment to prevent complications. Most patients require multiple treatment modalities, however with early diagnosis and treatment the majority of patients’ symptoms resolve.
D.1 Efficacy, safety, and tolerability of subcutaneous efgartigimod in chronic inflammatory demyelinating polyneuropathy: results from the ADHERE trial
Authors: Siddiqi Z , Allen JA , Basta I , Eggers C , Guptill J , Gwathmey K , Hewamadduma C , Hofman E , Hussain Y , Kuwabara S , Leypoldt F , Lin J , Lipowska M , Lowe M , Lauria Pinter G , Querol L , Suresh N , Chang T , Tse A , Ulrichts P , van Doorn PA , Van Hoorick B , Yamasaki R , Lewis RA
Published: May 2024

Background: Efgartigimod, a human immunoglobulin G (IgG)1 antibody Fc fragment, blocks the neonatal Fc receptor, decreasing IgG recycling and reducing pathogenic IgG autoantibody levels. ADHERE assessed the efficacy and safety of efgartigimod PH20 subcutaneous (SC; co-formulated with recombinant human hyaluronidase PH20) in chronic inflammatory demyelinating polyneuropathy (CIDP). Methods: ADHERE enrolled participants with CIDP (treatment naive or on standard treatments withdrawn during run-in period) and consisted of open-label Stage A (efgartigimod PH20 SC once weekly [QW]), and randomized (1:1) Stage B (efgartigimod or placebo QW). Primary outcomes were clinical improvement (assessed with aINCAT, I-RODS, or mean grip strength; Stage A) and time to first aINCAT score deterioration (relapse; Stage B). Secondary outcomes included treatment-emergent adverse events (TEAEs) incidence. Results: 322 participants entered Stage A. 214 (66.5%) were considered responders, randomized, and treated in Stage B. Efgartigimod significantly reduced the risk of relapse (HR: 0.394; 95% CI: 0.25–0.61) versus placebo (p=0.000039). Reduced risk of relapse occurred in participants receiving corticosteroids, intravenous or SC immunoglobulin, or no treatment before study entry. Most TEAEs were mild to moderate; 3 deaths occurred, none related to efgartigimod. Conclusions: Participants treated with efgartigimod PH20 SC maintained a clinical response and remained relapse-free longer than those treated with placebo.
D3 Interim results for Myasthenia Gravis-resource utilization, epidemiology, survival & treatment patterns (MG-REST) study in Ontario, Canada
Authors: Barnett-Tapia C , Quansah K , Erman A , Ng R , Nath N , Sharma A
Published: May 2024

Background: Reliable real-world data on the burden of MG is needed to inform Canadian clinical and policy decisions in the era of new MG therapeutics, including FcRn inhibitors. Given the lack of recent Canadian data on MG disease burden, the MG-REST Study aims to estimate the clinical burden of MG in Ontario. Methods: Ontario administrative data from ICES were utilized for a retrospective population-based cohort study of adults with MG identified through a validated algorithm (April 2013-March 2019) and followed for up to seven years (March 2020) to determine myasthenic crisis characteristics and overall survival (OS). Results: The MG cohort (n=2,601) had an average age of 65.7 years and 53.3% were males. Incidence of first myasthenic crisis was 9%, with 87% of events occurring at/after diagnosis. MG OS was 89%, 85% and 75% at 1-year, 2-years and 5-years, respectively, while OS after first crisis was 60%, 52%, and 39% for the same years. Conclusions: Despite the availability of conventional therapies throughout the study, MG crisis remains a serious, common complication of MG, with decreased survival at 1-year post-crisis (29% difference versus 1-year OS following MG diagnosis). Study highlights MG burden and unmet need for new effective therapies for MG treatment.
On wave-driven propulsion
Authors: Benham Graham P. , Devauchelle Olivier , Thomson Stuart J.
Published: May 2024

A theory is presented for wave-driven propulsion of floating bodies driven into oscillation at the fluid interface. By coupling the equations of motion of the body to a quasipotential flow model of the fluid, we derive expressions for the drift speed and propulsive thrust of the body which in turn are shown to be consistent with global momentum conservation. We explore the efficacy of our model in describing the motion of SurferBot (Rhee et al., Bioinspir. Biomim., vol. 17, issue 5, 2022), demonstrating close agreement with the experimentally determined drift speed and oscillatory dynamics. The efficiency of wave-driven propulsion is then computed as a function of driving oscillation frequency and the forcing location, revealing optimal values for both of these parameters which await confirmation in experiments. A comparison with other modes of locomotion and applications of our model with competitive water sports is discussed in conclusion.
CompLaw: A Coding Protocol and Database for the Comparative Study of Judicial Review
Authors: Gabel Matthew , Carrubba Clifford J. , Helmke Gretchen , Martin Andrew D. , Staton Jeffrey K. , Ward Dalston , Ziegler Jeffrey
Published: May 2024

A growing theoretical literature identifies how the process of constitutional review shapes judicial decision-making, legislative behavior, and even the constitutionality of legislation and executive actions. However, the empirical interrogation of these theoretical arguments is limited by the absence of a common protocol for coding constitutional review decisions across courts and time. We introduce such a coding protocol and database (CompLaw) of rulings by 42 constitutional courts. To illustrate the value of CompLaw, we examine a heretofore untested empirical implication about how review timing relates to rulings of unconstitutionality (Ward and Gabel 2019). First, we conduct a nuanced analysis of rulings by the French Constitutional Council over a 13-year period. We then examine the relationship between review timing and strike rates with a set of national constitutional courts in one year. Our data analysis highlights the benefits and flexibility of the CompLaw coding protocol for scholars of judicial review.
(Almost) complete characterization of the stability of a discrete-time Hawkes process with inhibition and memory of length two
Authors: Costa Manon , Maillard Pascal , Muraro Anthony
Published: May 2024

We consider a Poisson autoregressive process whose parameters depend on the past of the trajectory. We allow these parameters to take negative values, modelling inhibition. More precisely, the model is the stochastic process
$(X_n)_{n\ge0}$
with parameters
$a_1,\ldots,a_p \in \mathbb{R}$
,
$p\in\mathbb{N}$
, and
$\lambda \ge 0$
, such that, for all
$n\ge p$
, conditioned on
$X_0,\ldots,X_{n-1}$
,
$X_n$
is Poisson distributed with parameter
$(a_1 X_{n-1} + \cdots + a_p X_{n-p} + \lambda)_+$
. This process can be regarded as a discrete-time Hawkes process with inhibition and a memory of length p. In this paper we initiate the study of necessary and sufficient conditions of stability for these processes, which seems to be a hard problem in general. We consider specifically the case
$p = 2$
, for which we are able to classify the asymptotic behavior of the process for the whole range of parameters, except for boundary cases. In particular, we show that the process remains stochastically bounded whenever the solution to the linear recurrence equation
$x_n = a_1x_{n-1} + a_2x_{n-2} + \lambda$
remains bounded, but the converse is not true. Furthermore, the criterion for stochastic boundedness is not symmetric in
$a_1$
and
$a_2$
, in contrast to the case of non-negative parameters, illustrating the complex effects of inhibition.
Maize Landscapes in Indigenous Literatures: Toward Alternative Cartographic Imaginaries
Authors: Pigott Charles M.
Published: May 2024

The twentieth and twenty-first centuries have witnessed a literary revival in the Indigenous languages of the region known canonically as “Latin America.” Across this varied corpus, a major theme is the cultural significance of maize. This article compares the depiction of maize in four bilingual poems, each written in a different Indigenous language alongside Spanish: Nahuatl (Ethel Xochitiotzin Pérez), Yucatec Maya (María Dolores Dzul Barboza), Central Quechua (César Vargas Arce), and Southern Quechua (Emilio Corrales). Through close textual analysis and by recourse to theoretical perspectives such as “literary cartography,” the “textual continuum,” “deep mapping,” and “trans-indigeneity,” the article argues that each poem communicates culturally specific ways of understanding geography that, when set in dialogue, challenge hegemonic definitions of the Western Hemisphere such as North, South, or Latin “America.” Rather, the poems in combination weave an interconnected yet multiperspectival cartographic tapestry with maize as the common thread.
Moving to continuous classifications of bilingualism through machine learning trained on language production
Authors: Coco M. I. , Smith G. , Spelorzi R. , Garraffa M.
Published: May 2024

Recent conceptualisations of bilingualism are moving away from strict categorisations, towards continuous approaches. This study supports this trend by combining empirical psycholinguistics data with machine learning classification modelling. Support vector classifiers were trained on two datasets of coded productions by Italian speakers to predict the class they belonged to (“monolingual”, “attriters” and “heritage”). All classes can be predicted above chance (>33%), even if the classifier's performance substantially varies, with monolinguals identified much better (f-score >70%) than attriters (f-score <50%), which are instead the most confusable class. Further analyses of the classification errors expressed in the confusion matrices qualify that attriters are identified as heritage speakers nearly as often as they are correctly classified. Cluster clitics are the most identifying features for the classification performance. Overall, this study supports a conceptualisation of bilingualism as a continuum of linguistic behaviours rather than sets of a priori established classes.
Which older adults do not opt-in to Talking Therapies and why?
Authors: Prosser Rachel , Dosanjh Louisa , Jell Grace , Churchard Alasdair
Published: May 2024

Older adults are under-represented in Talking Therapies (previously named IAPT) services in the UK, a national priority for improvement in the NHS. A Talking Therapies service in the south of England identified that many older adults who were referred did not opt-in to assessment. We aimed to explore the characteristics of these older adults and understand their experiences, to inform recommendations to support them to opt-in to the service in future. First, demographic and referral characteristics were compared for older adults who did and did not opt-in, to explore any that increased odds of not opting-in. Next, surveys and semi-structured interviews were used to investigate older adults’ reasons for not opting-in. Responses were thematically analysed, and themes were categorised using the COM-B model to inform theory-based recommendations. Older age, being from an ethnic minority group, having a previous referral, not being able to receive text messages, and not self-referring (e.g. being referred by GP) all significantly increased the chances of older adults not opting-in. Thematic analysis found that impersonal and confusing processes, as well as older adults’ limited knowledge of Talking Therapies, beliefs about therapy, and physical, cognitive and life changes with age were barriers to opting-in. Several recommendations are made, including ideas to increase accessibility of information, change procedures to improve personal connection, and explore and overcome practical barriers. Improving routine data and feedback collection from people who do not opt-in will be important to inform and evaluate improvements.

Key learning aims

(1)

To recognise that the ongoing issue of under-representation of older adults within Talking Therapies extends beyond barriers to referral.

(2)

To understand demographic and referral characteristics that may increase the likelihood of older adults not opting-in to a Talking Therapies service following referral.

(3)

To understand the experiences of older adults who do not opt-in and the barriers they cite, exploring factors that impacted their capability, opportunity and motivation to opt-in.

(4)

To consider how services could change their procedures, information sharing, and community outreach to better serve older adults.
Reconstructing Glacier Surge Kinematics Using a Numerical Ice‐Flow Model Applied to the Dusty Glacier, St. Elias Mountains, Canada
Authors: Young Erik M. , Flowers Gwenn E. , Jiskoot Hester , Gibson H. Daniel
Published: May 2024

Long‐term records of the flow patterns and dynamics of surge‐type glaciers improve our understanding of their underlying dynamic processes, and are critical to better resolve their contribution to a changing cryosphere. We adapt a modeling approach designed to emulate glacier surging and fold kinematics using the full Stokes ice‐flow model Elmer/Ice to simulate surging of the Dusty Glacier, located in the St. Elias Mountains, Canada. We combine distributed mass‐balance and numerical ice‐flow models to reconstruct the fold kinematics of the 2001–2003 surge of the Dusty Glacier by comparing model results to Landsat‐7 and Sentinel‐2 imagery, and assess the sensitivity of centennial‐scale modeled glacier structure to different mass balance and sliding parameterizations. This study demonstrates the feasibility of using the approach to reconstruct the surface structure kinematics of a surge‐type glacier in nature, highlighting its potential application to other surge‐type glaciers and regions.
Spatiotemporal Variations of In Situ Vp/Vs Ratios During the 2019 Ridgecrest Earthquake Sequence Suggest Fault Zone Condition Changes
Authors: Lin Guoqing , Fan Wenyuan
Published: May 2024

The 2019 Mw 7.1 Ridgecrest earthquake was the largest event in California over the past 20 years. The earthquake was preceded by a sequence of foreshocks. However, the physical processes leading to the mainshock remain unclear. Here, we image the ratios of compressional (P)‐ to shear (S)‐wave velocity (Vp/Vs) in the fault zones and examine the spatial and temporal evolution of near‐source material properties during the Ridgecrest earthquake sequence. We find that the Vp/Vs ratios are spatially homogeneous in the rupture zones, indicating a lack of fault‐zone material difference along strike. We identify an anomalously low Vp/Vs ratio fault patch near the mainshock hypocenter before its occurrence, which returned to the background value after the earthquake. This low Vp/Vs ratio suggests fluid overpressure, which may have facilitated the nucleation of the 2019 Ridgecrest mainshock.
Responses of Field‐Aligned Currents and Equatorial Electrojet to Sudden Decrease of Solar Wind Dynamic Pressure During the March 2023 Geomagnetic Storm
Authors: Le Guan , Liu Guiping , Yizengaw Endawoke , Wu Chin‐Chun , Zheng Yihua , Vines Sarah , Buzulukova Natalia
Published: May 2024

We present the observations of field‐aligned currents and the equatorial electrojet during the 23 March 2023 magnetic storm, focusing on the effect of the drastic decrease of the solar wind dynamic pressure occurred during the main phase. Our observations show that the negative pressure pulse had significant impact to the magnetosphere‐ionosphere system. It weakened large‐scale field‐aligned currents and paused the progression of the storm main phase for ∼3 hr. Due to the sudden decrease of the plasma convection after the negative pressure pulse, the low‐latitude ionosphere was over‐shielded and experienced a brief period of westward penetration electric field, which reversed the direction of the equatorial electrojet. The counter electrojet was observed both in space and on the ground. A transient, localized enhancement of downward field‐aligned current was observed near dawn, consistent with the mechanism for transmitting MHD disturbances from magnetosphere to the ionosphere after the negative pressure pulse.
YOD1 protects against MRSA sepsis-induced DIC through Lys33-linked deubiquitination of NLRP3
Authors: Liu Chang , Fan Caihong , Liu Jia , Zhang Shiqi , Tang Huixin , Liu Yashan , Zhang Shengzheng , Wu Qiang , Zhang Jiandong , Qi Zhi , Shen Yanna
Published: May 2024

Disseminated intravascular coagulation (DIC) is considered to be the most common and lethal complication of sepsis. NLR-family pyrin domain-containing-3 (NLRP3) inflammasome plays an important role in host defense against microbial pathogens, and its deregulation may cause coagulation cascade and should be strictly managed. Here, we identified the deubiquitinase YOD1, which played a vital role in regulating coagulation in a NLRP3 inflammasome-dependent manner in sepsis induced by methicillin-resistant Staphylococcus aureus (MRSA). YOD1 interacted with NLRP3 to remove K33-linked ubiquitination of NLRP3 based on its deubiquitinating enzyme activity and specifically inhibited expression of NLRP3 as well as activation of NLRP3 inflammasome. Deficiency of YOD1 expression enhanced NLRP3 inflammasome activation and coagulation both in vitro and in vivo. In addition, pharmacological inhibition of the NLRP3 effectively improved coagulation and alleviated organ injury in Yod1−/− mice infected with MRSA. Thus, our study reported that YOD1 is a key regulator of coagulation during MRSA infection, and provided YOD1 as a potential therapeutic target for the treatment of NLRP3 inflammasome-related diseases, especially MRSA sepsis-induced DIC.
Revealing the hidden structure of disordered materials by parameterizing their local structural manifold
Authors: Hardin Thomas J. , Chandross Michael , Meena Rahul , Fajardo Spencer , Giovanis Dimitris , Kevrekidis Ioannis , Falk Michael L. , Shields Michael D.
Published: May 2024

Durable interest in developing a framework for the detailed structure of glassy materials has produced numerous structural descriptors that trade off between general applicability and interpretability. However, none approach the combination of simplicity and wide-ranging predictive power of the lattice-grain-defect framework for crystalline materials. Working from the hypothesis that the local atomic environments of a glassy material are constrained by enthalpy minimization to a low-dimensional manifold in atomic coordinate space, we develop a generalized distance function, the Gaussian Integral Inner Product (GIIP) distance, in connection with agglomerative clustering and diffusion maps, to parameterize that manifold. Applying this approach to a two-dimensional model crystal and a three-dimensional binary model metallic glass results in parameters interpretable as coordination number, composition, volumetric strain, and local symmetry. In particular, we show that a more slowly quenched glass has a higher degree of local tetrahedral symmetry at the expense of cyclic symmetry. While these descriptors require post-hoc interpretation, they minimize bias rooted in crystalline materials science and illuminate a range of structural trends that might otherwise be missed.
Relative dispersion ratios following fecal microbiota transplant elucidate principles governing microbial migration dynamics
Authors: Algavi Yadid M. , Borenstein Elhanan
Published: May 2024

Microorganisms frequently migrate from one ecosystem to another. Yet, despite the potential importance of this process in modulating the environment and the microbial ecosystem, our understanding of the fundamental forces that govern microbial dispersion is still lacking. Moreover, while theoretical models and in-vitro experiments have highlighted the contribution of species interactions to community assembly, identifying such interactions in vivo, specifically in communities as complex as the human gut, remains challenging. To address this gap, here we introduce a robust and rigorous computational framework, termed Relative Dispersion Ratio (RDR) analysis, and leverage data from well-characterized fecal microbiota transplant trials, to rigorously pinpoint dependencies between taxa during the colonization of human gastrointestinal tract. Our analysis identifies numerous pairwise dependencies between co-colonizing microbes during migration between gastrointestinal environments. We further demonstrate that identified dependencies agree with previously reported findings from in-vitro experiments and population-wide distribution patterns. Finally, we explore metabolic dependencies between these taxa and characterize the functional properties that facilitate effective dispersion. Collectively, our findings provide insights into the principles and determinants of community dynamics following ecological translocation, informing potential opportunities for precise community design.
Spider-silk-inspired strong and tough hydrogel fibers with anti-freezing and water retention properties
Authors: Wu Shaoji , Liu Zhao , Gong Caihong , Li Wanjiang , Xu Sijia , Wen Rui , Feng Wen , Qiu Zhiming , Yan Yurong
Published: May 2024

Ideal hydrogel fibers with high toughness and environmental tolerance are indispensable for their long-term application in flexible electronics as actuating and sensing elements. However, current hydrogel fibers exhibit poor mechanical properties and environmental instability due to their intrinsically weak molecular (chain) interactions. Inspired by the multilevel adjustment of spider silk network structure by ions, bionic hydrogel fibers with elaborated ionic crosslinking and crystalline domains are constructed. Bionic hydrogel fibers show a toughness of 162.25 ± 21.99 megajoules per cubic meter, comparable to that of spider silks. The demonstrated bionic structural engineering strategy can be generalized to other polymers and inorganic salts for fabricating hydrogel fibers with broadly tunable mechanical properties. In addition, the introduction of inorganic salt/glycerol/water ternary solvent during constructing bionic structures endows hydrogel fibers with anti-freezing, water retention, and self-regeneration properties. This work provides ideas to fabricate hydrogel fibers with high mechanical properties and stability for flexible electronics.
Unusual facet and co-catalyst effects in TiO2-based photocatalytic coupling of methane
Authors: Zhang Huizhen , Sun Pengfei , Fei Xiaozhen , Wu Xuejiao , Huang Zongyi , Zhong Wanfu , Gong Qiaobin , Zheng Yanping , Zhang Qinghong , Xie Shunji , Fu Gang , Wang Ye
Published: May 2024

Photocatalytic coupling of methane to ethane and ethylene (C2 compounds) offers a promising approach to utilizing the abundant methane resource. However, the state-of-the-art photocatalysts usually suffer from very limited C2 formation rates. Here, we report our discovery that the anatase TiO2 nanocrystals mainly exposing {101} facets, which are generally considered less active in photocatalysis, demonstrate surprisingly better performances than those exposing the high-energy {001} facet. The palladium co-catalyst plays a pivotal role and the Pd2+ site on co-catalyst accounts for the selective C2 formation. We unveil that the anatase {101} facet favors the formation of hydroxyl radicals in aqueous phase near the surface, where they activate methane molecules into methyl radicals, and the Pd2+ site participates in facilitating the adsorption and coupling of methyl radicals. This work provides a strategy to design efficient nanocatalysts for selective photocatalytic methane coupling by reaction-space separation to optimize heterogeneous-homogeneous reactions at solid-liquid interfaces.
Study of plasmid mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from pediatric patients with sepsis
Authors: Gomaa Elsayed Ahmed , M Fahmy Ehab , Abdellatif Alsayed Mona , Ahmed Mai Essam , El Sayed Zaki Maysaa , Mofreh Mohamed Mohamed
Published: May 2024

The resistance to antibiotics in Gram-negative bacilli causing sepsis is a warning sign of failure of therapy. Klebsiella pneumoniae (K. pneumoniae) and Escherichia coli (E. coli) represent major Gram-negative bacilli associated with sepsis. Quinolone resistance is an emerging resistance among E. coli and K. pneumoniae. Therefore, the present study aimed to study the presence of plasmid-mediated quinolone resistance (PMQR) genes qnrA, qnrB, and qnrS by polymerase chain reaction (PCR) in E. coli and K. pneumoniae isolated from pediatric patients with sepsis. This was a retrospective cross-sectional study that included pediatric patients with healthcare-associated sepsis. The E. coli and K. pneumoniae isolates were identified by microbiological methods. PMQR genes namely qnrA, qnrB, and qnrS were detected in E. coli and K. pneumoniae isolates by PCR. The results were analyzed by SPPS24, and the qualitative data was analyzed as numbers and percentages and comparison was performed by Chi-square test, P was significant if < 0.05. The most prevalent gene detected by PCR was qnrA (75%), followed by qnrB (28.1%), and qnrS (25%). The most frequently detected qnr gene in E coli and K. pneumoniae was qnrA (28.8%, and 16.3% respectively). The present study highlights the high prevalence of ciprofloxacin resistance among E. coli and K. pneumoniae isolated from pediatric patients with healthcare-associated sepsis. There was a high frequency of PMQR genes in E. coli and K. pneumoniae isolated from pediatric patients. Therefore, it is important to monitor the spread of PMQR genes in clinical isolates to ensure efficient antibiotic use in those children. The finding denotes the importance of an antibiotics surveillance program.
Shifts in uterine microbiome associated with pregnancy outcomes at first insemination and clinical cure in dairy cows with metritis
Authors: Figueiredo Caio C. , Monteiro Hugo F. , Cunha Federico , Bisinotto Danilo Z. , Ruiz Angel Revilla , Duarte Gustavo A. , Ge Yong , Lima Fábio S. , Mohamadzadeh Mansour , Galvão Klibs N. , Bisinotto Rafael S.
Published: May 2024

Objectives were to assess differences in uterine microbiome associated with clinical cure and pregnancy outcomes in dairy cows treated for metritis. Cows with metritis (reddish-brownish, watery, and fetid vaginal discharge) were paired with cows without metritis based on parity and days postpartum. Uterine contents were collected through transcervical lavage at diagnosis, five days later following antimicrobial therapy (day 5), and at 40 days postpartum. Uterine microbiome was assessed by sequencing the V4 hypervariable region of the 16S rRNA gene. Although alpha-diversity based on Chao1, Shannon, and inverse Simpson indexes at diagnosis did not differ between cows with and without metritis, disease was associated with differences in beta-diversity. Prevalence of Porphyromonas, Bacteroides, and Veillonella was greater in cows with metritis. Streptococcus, Sphingomonas, and Ureaplasma were more prevalent in cows without metritis. Differences in beta-diversity between cows with and without metritis persisted on day 5. Uterine microbiome was not associated with clinical cure. Richness and alpha-diversity, but not beta-diversity, of uterine microbiome 40 days postpartum were associated with metritis and pregnancy. No relationship between uterine microbiome and pregnancy outcomes was observed. Results indicate that factors other than changes in intrauterine bacterial community underlie fertility loss and clinical cure in cows with metritis.
Research collaboration data platform ensuring general data protection
Authors: Toma Monica , Bönisch Caroline , Löhnhardt Benjamin , Kelm Michael , Bohnenberger Hanibal , Winkelmann Sven , Ströbel Philipp , Kesztyüs Tibor
Published: May 2024

Translational data is of paramount importance for medical research and clinical innovation. It has the potential to benefit individuals and organizations, however, the protection of personal data must be guaranteed. Collecting diverse omics data and electronic health records (EHR), re-using the minimized data, as well as providing a reliable data transfer between different institutions are mandatory steps for the development of the promising field of big data and artificial intelligence in medical research. This is made possible within the proposed data platform in this research project. The established data platform enables the collaboration between public and commercial organizations by data transfer from various clinical systems into a cloud for supporting multi-site research while ensuring compliant data governance.
Technologically advanced running shoes reduce oxygen cost and cumulative tibial loading per kilometer in recreational female and male runners
Authors: Werkhausen Amelie , Lund-Hansen Magne , Wiedenbruch Lucas , Peikenkamp Klaus , Rice Hannah
Published: May 2024

Technologically advanced running shoes (TARS) improve performance compared to classical running shoes (CRS). Improved race performance has been attributed to metabolic savings in male runners, but it remains unclear if these same benefits are experienced among females and in recreational runners. The mechanisms behind these benefits are still not fully understood despite the need for optimisation, and their influence on injury mechanisms has not been explored. Here we combined biomechanical, physiological, and modelling approaches to analyse joint mechanics, oxygen uptake, and tibial load in nineteen male and female recreational runners running with CRS and TARS at their individual lactate threshold speed (12.4 ± 1.9 km/h). Oxygen uptake was 3.0 ± 1.5% lower in TARS than in CRS. Ankle dorsiflexion, joint moment and joint power were reduced in TARS compared to CRS at various phases of stance including midstance, while knee joint mechanics were mostly similar throughout. There were no significant differences for tibial bending moment during the stance phase but cumulative tibial damage per kilometre was 12 ± 9% lower in TARS compared to CRS. Our results suggest that running with TARS reduces oxygen cost in recreational female and male runners, which may partly be explained by differences in lower limb joint mechanics. The lower cumulative tibial bone load with TARS may allow runners to run longer distances in this type of shoe compared to CRS.
Intravenous versus oral ‘l-ornithine-l-aspartate’ in overt hepatic encephalopathy: a randomized comparative study
Authors: Jhajharia Ashok , Singh Shashank , Jana Sangeeta , Ashdhir Prachis , Nijhawan Sandeep
Published: May 2024

Hepatic encephalopathy (HE), a morbid ordeal affecting chronic liver disease patients always insists for the search of a rational, superior & infallible agent beyond the time-proven standards i.e., Lactulose & Rifaximin. In this RCT, we compared the efficacy of intravenous (IV) l-ornithine-l-aspartate(LOLA) versus Oral LOLA in patients with chronic liver disease(CLD) enduring overt Hepatic Encephalopathy(OHE). 40 CLD patients with OHE were randomly assigned IV or oral LOLA in a 1:1 ratio. Patients were graded for HE and monitored for serum ammonia levels from day 1 to day 5. The aim was to compare IV versus oral LOLA efficacy in HE grades improvement and its correlation with ammonia levels. The study was registered with clinical trials registry-India, CTRI/2020/12/029943. Baseline characteristics of patients in both groups were similar. The mean difference in ammonia levels from day 1 to day 5 was 55.4 ± 32.58 µmol/L in the IV LOLA group and 60.75 ± 13.82 µmol/L in the oral LOLA group (p = 0.511). Significant reductions in ammonia levels were observed from day 1 to day 5 within each group (p < 0.001). HE grade & ammonia correlated positively in both groups. LOLA, regardless of administration route, has demonstrated efficacy in OHE.
Development and validation of early prediction models for new-onset functional impairment in patients after being transferred from the ICU
Authors: Xiao Zewei , Zeng Limei , Chen Suiping , Wu Jinhua , Huang Haixing
Published: May 2024

A significant number of intensive care unit (ICU) survivors experience new-onset functional impairments that impede their activities of daily living (ADL). Currently, no effective assessment tools are available to identify these high-risk patients. This study aims to develop an interpretable machine learning (ML) model for predicting the onset of functional impairment in critically ill patients. Data for this study were sourced from a comprehensive hospital in China, focusing on adult patients admitted to the ICU from August 2022 to August 2023 without prior functional impairments. A least absolute shrinkage and selection operator (LASSO) model was utilized to select predictors for inclusion in the model. Four models, logistic regression, support vector machine (SVM), random forest (RF), and extreme gradient boosting (XGBoost), were constructed and validated. Model performance was assessed using the area under the curve (AUC), accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV). Additionally, the DALEX package was employed to enhance the interpretability of the final models. The study ultimately included 1,380 patients, with 684 (49.6%) exhibiting new-onset functional impairment on the seventh day after leaving the ICU. Among the four models evaluated, the SVM model demonstrated the best performance, with an AUC of 0.909, accuracy of 0.838, sensitivity of 0.902, specificity of 0.772, PPV of 0.802, and NPV of 0.886. ML models are reliable tools for predicting new-onset functional impairments in critically ill patients. Notably, the SVM model emerged as the most effective, enabling early identification of patients at high risk and facilitating the implementation of timely interventions to improve ADL.
Prognosis and influencing factors of ER-positive, HER2-low breast cancer patients with residual disease after neoadjuvant chemotherapy: a retrospective study
Authors: Tang Lingfeng , Jiang Linshan , Shu Xiujie , Jin Yudi , Yu Haochen , Liu Shengchun
Published: May 2024

Previously, we found that patients with estrogen receptor (ER)-positive, HER2-low breast cancer are resistant to neoadjuvant chemotherapy (NACT) and have worse outcomes than those who achieve pathological complete response (pCR) after NACT. This study aimed to investigate the prognosis and influencing factors in these patients. A total of 618 patients with ER-positive breast cancer who received standard thrice-weekly NACT were enrolled, including 411 patients with ER-positive, HER2-low breast cancer. Data on the clinicopathological features of these patients before and after NACT were collected. Univariate and multivariate Cox regression analyses were used to identify the independent factors affecting 5-year disease-free survival (DFS). Among the ER-positive, HER2-low patients, 49 (11.9%) achieved a pCR after NACT. A significant difference in survival was observed between patients with and without residual disease after NACT. Additionally, changes in immunohistochemical markers and tumor stages before and after NACT were found to be significant. According to univariate and multivariate analyses, cN_stage (P = 0.002), ER (P = 0.002) and Ki67 (P = 0.023) expression before NACT were significantly associated with 5-year DFS, while pT_stage (P = 0.015), pN_stage (P = 0.029), ER (P = 0.020) and Ki67 (P < 0.001) levels after NACT were related to 5-year DFS in ER-positive, HER2-low patients with residual disease. Our study suggested that high proliferation, low ER expression and advanced stage before and after NACT are associated with a poor prognosis, providing useful information for developing long-term treatment strategies for ER-positive, HER2-low breast cancer in patients with residual disease in the future.
The manatee variational autoencoder model for predicting gene expression alterations caused by transcription factor perturbations
Authors: Yang Ying , Seninge Lucas , Wang Ziyuan , Oro Anthony , Stuart Joshua M. , Ding Hongxu
Published: May 2024

We present the Manatee variational autoencoder model to predict transcription factor (TF) perturbation-induced transcriptomes. We demonstrate that the Manatee in silico perturbation analysis recapitulates target transcriptomic phenotypes in diverse cellular lineage transitions. We further propose the Manatee in silico screening analysis for prioritizing TF combinations targeting desired transcriptomic phenotypes.
Evolution characteristics and multi-scenario prediction of habitat quality in Yulin City based on PLUS and InVEST models
Authors: Li Shifeng , Hong Zenglin , Xue Xuping , Zheng Xiaofeng , Du Shaoshao , Liu Xiaofeng
Published: May 2024

As a major energy city in China, Yulin City has faced huge challenges to the ecological environment with its rapid economic development and rapid urbanization. Therefore, it is of great significance to study the impact of land use changes on habitat quality. Based on three periods of land use data in Yulin City in 1995, 2005 and 2015, the PLUS model was used to simulate the land use changes in 2015. The measured kappa coefficient was 0.8859, which met the simulation accuracy requirements. By setting development zone boundaries and adjusting parameters, three progressive scenarios are designed to predict the spatial distribution of land use in Yulin City in 2035. The InVEST model was used to analyze the spatiotemporal evolution of Yulin City’s habitat quality in the past 20 years and evaluate the distribution of Yulin City’s habitat quality under three scenarios after 20 years. The results are as follows: (1) During the study period, construction land in Yulin City expanded rapidly, with an area increase of 380.87 km2 in 20 years, and ecological land gradually shrank. (2) The land use simulation results of Yulin City under various scenarios in 2035 show that future land use changes in Yulin City will mainly be concentrated in the central and western regions. (3) During the study period, the habitat quality of Yulin City was at a medium level and the overall habitat quality showed a downward trend. Spatially, the degree of habitat quality degradation in Yulin City showed a characteristic of gradually decreasing from West to East. (4) By 2035, under the scenario of suitable urban economic development, Yulin City’s habitat quality has been improved to a certain extent, which not only protects ecological security but also meets the demand for construction land for urban development. The results of this study help the government better understand the evolution of land use and habitat quality in Yulin City in the past 20 years, and provide theoretical support and reference for the formulation of Yulin City’s ecological environment protection policies and the implementation of ecological protection work under the current land spatial planning.
Optimizing hematite filter cake treatment using reducing agents
Authors: Siddig Osama , Elkatatny Salaheldin , Alafnan Saad
Published: May 2024

In drilling operations, the formation of a filter cake is crucial for well stability, but its removal post-drilling is essential to restore rock formation productivity. This study focuses on hematite-based filter cakes and investigates factors influencing their solubility and removal, addressing a significant knowledge gap in the field. The research methodology involves examining the effects of various factors, including types and concentrations of reducing agents, temperature, particle size, and treatment duration, on the dissolution process. Notably, Nuclear Magnetic Resonance (NMR) tests are employed to assess the treatment's impact on core porosity. Among the diverse reducing agents examined, ferrous chloride emerges as the optimal choice for effectively enhancing hematite solubility. Particularly, a composite solution of ferrous chloride (10 wt.%) and hydrochloric acid (6 wt.%), was highly efficient demonstrated by exhibiting rapid solubilization of hematite filter cakes. A removal efficiency of approximately 99%, with a parallel enhancement in core permeability was achieved. NMR tests reveal the treatment's success in reinstating the porosity system, which had undergone reduction due to drilling fluid particles. Crucially, the solution exhibits a considerably lower corrosion rate than concentrated hydrochloric acid, highlighting its potential to mitigate environmental concerns while ensuring efficient filter cake removal. The findings of this research provide valuable insights into optimizing post-drilling operations, balancing environmental sustainability and operational efficiency. The identified composite solution offers a promising approach to efficient filter cake removal while mitigating environmental concerns associated with corrosion. Overall, this study contributes to advancing the understanding and practice of well productivity enhancement in the oil and gas industry.