bluuebunny/crossref_metadata_2025 huggingface dataset:
Have river flow droughts become more severe? A review of the evidence from the UK – a data-rich temperate environment
Authors: Hannaford Jamie , Turner Stephen , Chevuturi Amulya , Chan Wilson , Barker Lucy J. , Tanguy Maliko , Parry Simon , Allen Stuart
Published: October 2024

Abstract. When extreme hydrological events (floods and droughts) occur, there is inevitably speculation that such events are a manifestation of anthropogenic global warming. The UK is generally held as a wet country, but recent drought events in the UK have led to growing concerns around droughts becoming more severe – for sound scientific reasons, given physical reasoning and projections for future. In this extended review, we ask whether such claims are reasonable for hydrological droughts in the UK, using a combination of literature review and extended analysis. The UK has a well-established monitoring programme and a very dense body of research to call on, and hence provides a good international case study for addressing this question. We firstly assess the evidence for changes in the well-gauged post-1960 period, before considering centennial scale changes using published reconstructions. We then seek to provide a synthesis of the state-of-the-art in our understanding of the drivers of change, both climatic and in terms of direct human disturbances to river catchments (e.g. changing patterns of water withdrawals, impoundments, land use changes). These latter impacts confound the identification of climate-driven changes, and yet human impacts are themselves increasingly recognised as potential agents of changing drought regimes. We find little evidence of compelling changes towards worsening drought, apparently at odds with climate projections for the relatively near future and widely-held assumptions of the role of human disturbances in intensifying droughts. Scientifically, this is perhaps unsurprising (given uncertainties in future projections, and the challenge of identifying signals in short, noisy records, and a lack of datasets to quantify human impacts) but it presents challenges to water managers and policymakers. We dissect some of the reasons for this apparent discrepancy and set out recommendations for guiding research and policy alike. While our focus is the UK, we envisage the themes within will resonate with the international community and we consider ways our findings are relevant more broadly, as well as how the UK can learn from the global community.
Intra-operative and post-operative complications of endometriosis excision using the SOSURE approach — A single- surgeon retrospective series of 1116 procedures over 8 years
Authors: Khazali S , Bachi A , Mondelli B , Fleischer K , Adamczyk M , Delanerolle G , Shi J.Q. , Yang X , Nisar P , Bearn P
Published: October 2024

Background: Endometriosis surgery outcomes have been widely studied, yet heterogeneity in terminology and techniques persist.

Objectives: This study focuses on the perioperative outcomes of a single surgeon using the same structured approach (SOSURE: Survey & Sigmoid mobilisation, Ovarian mobilisation, Suspension of uterus and ovaries, Ureterolysis, Rectovaginal and pararectal space development, Excision of all visible disease) and adheres to the recent standardised terminology proposed by international gynaecological and endometriosis societies.

Materials and methods: A quality improvement study was conducted retrospectively from January 2015 to January 2023. Data collection involved two databases: the National British Society for Gynaecological Endoscopy (BSGE) database and a more comprehensive locally kept database. The methodology also integrated four endometriosis staging systems.

Main outcome measures: Intra-operative and post-operative complication rates.

Results: Between 2015 and 2023, 1047 women underwent 1116 endometriosis procedures in various UK hospitals with S.K. as primary surgeon. Exclusions totalled 20 due to missing records and specific surgical criteria. The rate of major post-operative complications (Clavien-Dindo grade 3a and 3b) was 1.5% and minor post-operative complications (Clavien-Dindo grade 1 and 2) were seen in 13.8%. No Clavien-Dindo grade 4 or 5 complications were noted.

Conclusion: Our study has shown a low complication rate in endometriosis surgery, despite increasing complexity of surgical cases. This is likely attributed to the surgeon’s learning curve, high surgical volume and adherence to a structured approach.

What’s new? Our study demonstrates the learning curve of a surgeon over the course of 8 years. This series involved more than 1000 patients and to our knowledge, is the first to report the complexity of the casemix using four different endometriosis staging systems.
Trends in Library Services and the competencies of the librarian
Authors: Karina Lira Da Silva Edna , Dos Santos Andrey Anderson , Maria dos Santos Bahia Jacintho Eliana , Marques Chaíça Beatriz
Published: October 2024

This study sought to discuss the perspective of services for libraries and to indicate the competences of professionals to act in face of this scenario. Regarding the nature of the research, it is characterized as basic research, using an exploratory approach and bibliographic and documental characteristics. The searches were carried out in the following databases: Web of Science, Scopus (Elsevier), SciELO. The 2,030 documents were retrieved; these were explored by reading the titles, the keywords, and the abstract. In this way, we evaluated which of the articles answered the research question. Among the documents checked, 55 documents were selected. The results identified services in the technological sphere: Artificial Intelligence (AI), the Internet of Things, Drones, Virtual Assistants, and Blockchain. In the society, sphere Co-working. In the Education category, Badging, and in the Environment category, Resilience. Given what was proposed, the librarian’s perception of these changes becomes essential as actors and providers of these services.
Information behaviour of Deaf people in university libraries
Authors: Coelho França Maísa , Furnival Ariadne Chloe Mary
Published: October 2024

In Brazil, there are more than 10 million Deaf people. Of these, 2,592 Deaf people are enrolled in Higher Education institutions. This pilot study aims to explore how the field of Library and Information Science (LIS) has studied Deaf people in university libraries. This research uses a mixed methods approach: the first part of the study uses a descriptive approach to present the current state of LIS literature on Deaf users of university libraries. The second part of the research is empirical and consists of a survey that has yet to be fully carried out. It also suggests how the university library can contribute to the empowerment of the deaf community.
Subject Analysis, Content Analysis and Domain Analysis
Authors: Alves Silva Portella Tauany Lorena , De Lima Gercina Ângela
Published: October 2024

From the perspective of the constant increase in data and information, consider in Library and Information Science that the correct analysis and representation of the contents of documents analyzed in specific domains is essential for the retrieval, organization, and dissemination of information. Subject Analysis categorizes topics and details, making it easier to retrieve relevant information. Domain Analysis studies specific characteristics of a field of knowledge, comprising terminologies and concepts. Content Analysis identifies and analyzes textual elements, deepening the understanding of documentary content. This study explores these analyses' approaches, techniques, and methodologies, highlighting their often confused interrelationships, differences, and similarities. To achieve the proposed objective to support the conceptual and theoretical-methodological discussion on subject analysis, content analysis, and domain analysis, focusing on their interrelations, differences, and similarities that are often confused in their concepts and methodologies, the research developed an exploratory and descriptive approach, a bibliographic survey was carried out in the BRAPCI database, using the terms "domain analysis", "content analysis" and "content analysis", recovering 134 documents. Results are efficiently defined and applied to each analysis. These analyses guarantee efficient information retrieval, which is vital to growing data volume.
Exploring and depicting information pathologies
Authors: Vicente Paulo
Published: October 2024

This study addresses two information pathologies: information technology anxiety (ITA) and information overload (IO). This research aims to determine the level of both ITA and IO amongst the undergraduate students of Information Science of the Faculty of Arts and Humanities of the University of Coimbra, Portugal, and to ascertain how both phenomena correlate with students’ gender, age, number of enrolments in the degree and average frequency of use of the university’s libraries throughout the academic year. Concerning the research methodology, we opted for exploratory and descriptive research with a quantitative approach and adopted the case study and questionnaire survey methods. Both descriptive and inferential statistics were used as methods of data analysis. The findings, concerning 39\% of the population under study (N = 88; n = 34), reveal a considerably low prevalence of ITA but a substantial occurrence of IO. Both ITA and IO correlate positively with the female gender. Students aged 19 and 24 years are the least likely to manifest both ITA and IO; in turn, students aged 22 years are the most likely to simultaneously show ITA and IO. Both phenomena tend to increase as students progress through the degree. Last, a higher frequency of use of the university’s libraries is associated with a higher level of ITA and IO. Our findings emphasize the high incidence of IO in university students and the need for increased skills in all dimensions of the human-information-technology relationship/system, particularly in the information filtering and management areas.
EL COMUNISMO INOCENTE. LA GUERRA CULTURAL GRAMSCIANO-HEGELIANA CONTRA LA SOCIEDAD ABIERTA
Authors: Pedro Fraile Balbín
Published: October 2024

La última vez que oí a Carlos Rodríguez Braun hablar en público sobre liberalismo fue en la Fundación Rafael del Pino, en un acto organizado por el Centro Diego de Covarrubias a finales de enero de 2020. Impartía una conferencia1 sobre liberalismo y religión. Al final se suscitó una avalancha de preguntas y, como siempre que habla él, un debate fogoso. En el último momento, cuando el moderador cerraba el debate, ya sin tiempo para contestar, un joven muy tímido preguntó desde el fondo “¿por qué los enemigos de la libertad se sienten moralmente superiores?”. La pregunta quedó sin respuesta- ¿Por qué la izquierda se siente moralmente superior? O, mejor dicho, ¿por qué tantos conservadores liberales han asumido la superioridad moral de la izquierda colectivista? Este ensayo tiene como propósito sugerir una respuesta a esa pregunta.
RESEÑA DEL LIBROECONOMIC FREEDOM AND SOCIAL JUSTICE de Wanjiru Njoya
Authors: Walter Block
Published: October 2024

Estoy de acuerdo con aproximadamente el 99% de lo que escribe esta autora. Tengo dos alternativas y, créanme, la primera me tienta enormemente, pero no la seguiré. Sería simplemente citar ampliamente este libro y felicitar a la autora por exponer puntos incisivos, válidos y significativos a lo largo del camino. Citaría prácticamente la totalidad del libro y tal vez proporcionaría tono y variaciones sobre lo que escribe. La segunda es centrarse en las raras ocasiones en las que discrepamos. Yo he elegido esta última. Es más divertida. Aprenderé más al hacerlo.1 Y, tal vez, posiblemente, de esta manera podamos juntos mejorar lo que ya es una magnífica contribución.
Eksistensi Pusat Organisasi Kemasyarakatan (POK) Masyarakat Menyikapi Pemilu 2024
Authors: Purba Lilis , Selan Medrys , Mubarok Ibnu
Published: October 2024

Pemilihan Umum (Pemilu) di Indonesia adalah manifestasi konkrit dari nilai-nilai demokrasi, sesuai dengan Pancasila dan UUD 1945. Sejak pelaksanaannya yang pertama pada tahun 1955, Pemilu telah mengalami sejumlah evolusi dalam aspek hukum, tahapan, dan penyelenggaraan. Dalam menyongsong Pemilu 2024, dinamika baru telah muncul, termasuk perubahan dalam teknologi dan preferensi politik masyarakat. Salah satu elemen kunci dalam lanskap pemilihan ini adalah Pusat Organisasi Kemasyarakatan (POK). Penelitian ini menyoroti peran dan eksistensi POK dalam menghadapi tantangan Pemilu 2024. Melalui metode studi teoritis dasar dengan pendekatan kualitatif, penelitian ini mengkaji posisi, peran, dan tantangan yang dihadapi POK dalam konteks demokrasi Indonesia. Hasil menunjukkan bahwa POK memegang peran vital sebagai mediator antara masyarakat dan aparatur pemerintahan, dengan ekspektasi tinggi untuk berperan aktif dalam memastikan keadilan dan integritas Pemilu 2024.
Induction of mineralized matrix production by recombinant human BMP-2 Immobilized in TEMPO-Oxidized Cellulose Hydrogel: a novel target for tissue repair
Authors: Domingos Neto Benedito , Hebling Victória Maria , Gomes Cardoso Gastaldi Gabriela , Sobreiro Selistre de Araújo Heloisa , Trovatti Eliane , Rosas da Costa Iemma Mônica
Published: October 2024

Bone morphogenetic proteins (BMPs) are potent promoters of osteogenesis, especially BMP-2, which has been highlighted for acting as a growth and differentiation factor that promotes new bone formation. There are several biomaterials that can be used to release bioactive substances, such as natural polymers. Cellulose has stood out for the possibility of its chemical modification using the reagent 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) to obtain a cellulose derivative (TEMPO oxidized cellulose nanofibers - ToCNF), which is shown to be a promising material for biological application. The objective of this work was to evaluate TEMPO cellulose immobilized with rhBMP-2 against the activity of inducing bone cell proliferation and differentiation in vitro, evaluating the ability to form bone matrix in pre-osteoblastic cell lineage of rats - MC3T3. Cell viability assays using resazurin were performed and for detection of mineralized matrix, Alizarin Red solution was used. The results reveal the good capacity of TEMPO cellulose functionalized with rhBM-2 in inducing the synthesis of mineralized bone matrix.
PROTECTIVE EFFECTS OF ANTIOXIDANTS AGAINST BURN-RELATED INJURIES
Authors:   Department of Physiology and Pathophysiology, Faculty of Medicine, Medical University-Varna, Bulgaria., Hristova Minka
Published: October 2024

Thermal burn injuries are still a serious public health concern. Severe burns cause both local skin and distant-organ injuries. Many pathological mechanisms are involved in burn-induced injuries. Overproduction of reactive oxygen species (ROS) and oxidative stress play key roles in the pathogenesis of the systemic response and serious complications after burns. Antioxidants are intensively studied, and their beneficial effects are documented. Regardless of that, they still attract the researchers' interest in uncovering new evidence about their favorable impact on burn injuries. This review aims to describe recent evidence related to the mechanisms of tissue protection after administering some antioxidants with biogenic origins in burns.
Lipid metabolism parameters and alcohol consumption among men in Arkhangelsk, Russia: The Know Your Heart study
Authors: Guseinova U. G. , Mitkin N. A. , Solovieva N. V. , Soloviev A. G. , Mirolyubova O. A. , Malyutina S. K. , Kudryavtsev A. V.
Published: October 2024

Aim. To assess the association between levels of alcohol consumption and lipid metabolism parameters among adult men.

Materials and methods. The study included 881 men aged 35–69 from the general population of Arkhangelsk, who participated in the 2015–17 Know Your Heart study, and 161 men who received inpatient treatment for alcohol-related diagnoses (narcology patients). Participants were divided into five levels of alcohol consumption: non-drinkers, non-problem (infrequent moderate consumption), hazardous (frequent consumption in doses hazardous to health), harmful (prenosological), and narcology patients. Using multivariate linear regressions, we analyzed differences between these groups in atherogenic lipid fractions (total cholesterol [TC], triglycerides [TG], low-density lipoprotein [LDL], apolipoprotein B [ApoB], remnant cholesterol, and non-HDL cholesterol, lipoprotein(a)) (Lp(a)), antiatherogenic lipid fractions (high-density lipoprotein [HDL], apolipoprotein A1 [апо A1]), and in ApoB/апо A1 ratio.

Results. Compared with non-problem drinkers, hazardous drinkers had higher mean levels of HDL by 0.22 mmol/L, HDL by 0.07 mmol/L, and ApoB by 0.04 g/L. Among harmful drinkers, mean HDL was higher by 0.15 mmol/L and апо A1 by 0.08 g/L, but the ApoB/апо A1 ratio was lower by 0.06. Among narcology patients, mean TC levels were lower by 0.42 mmol/L, LDL by 0.41 mmol/L, ApoB by 0.09 g/L, ApoB/апо A1 by 0.08, and non-HDL by 0.45 mmol/L, but TG was higher by 0.15 mmol/L. Lp(a) in this group was higher by 0.29 mg/dl only after adjustment for markers of liver function. Non-drinkers had on average lower levels of TC by 0.29 mmol/L, HDL by 0.11 mmol/L, and апо A1 by 0.08 g/L.

Conclusions. Compared with non-problem drinkers, hazardous drinkers had elevated levels of both atherogenic and antiatherogenic lipid fractions, hazardous drinkers had only elevated levels of antiatherogenic fractions, and narcology patients had the lowest levels of atherogenic lipid fractions but elevated TG levels. Therefore, lipid profiles may reflect the level of alcohol consumption, which should be taken into account when assessing cardiovascular risk.
Healthy nutrition – prevention of chronic non-communicable diseases. Overview of recommendations
Authors: Swarovskaya P. K. , Tsygankova D. P. , Bazdyrev E. D.
Published: October 2024

The article provides an overview of the National Recommendations on healthy nutrition available in Europe, Asia (China), the USA and the Russian Federation, assesses the compliance of the presented guidelines with the principles of a healthy diet according to the recommendations of the World Health Organization, and analyzes their role in the prevention of the development of non-infectious diseases, including number of diseases of the cardiovascular system. A search of relevant literature for the review was conducted in PubMed (www.pubmed.gov) using English keywords (“national dietary guidelines”, “WHO diet”, “FBDG”, “dietary guidelines”, “cardiovascular factors”), the website of the National Project of the Russian Federation “Healthy Nutrition” (https://здоровое-питание.рф), eLIBRARY.RU (https://elibrary.ru/defaultx.asp) using keywords in Russian (“ESSE-RF”, “risk factors for non-infectious diseases”, “nutrition”). Publications published between 1995 and 2022 were considered. The review included the National Recommendations in force for 2023, as well as the results of cohort and multicenter studies, the purpose of which was to assess the impact of current recommendations on the development of traditional cardiovascular risk factors, associated clinical conditions (myocardial infarction, stroke, etc.), as well as the impact on general mortality. Publications with completely duplicate data and conclusions were excluded, and in case of partial overlap, both sources were indicated. As a result, a list was compiled of articles, newsletters, and links with up-to-date information on the National Guidelines.
Experimental Investigation of Two-Pass Solar Dryer with V-Corrugated Absorber for Potato Slice Drying
Authors: Gajbhiye Manjeet , Kalbande Vijay , Bankar Amit , Baseshankar Manoj , Fande Ashish
Published: October 2024

Reducing the moisture level of food items can help prevent bacterial development and deterioration, extend shelf life, reduce packaging, and improve storage for convenient transportation. In this paper, a two-pass solar dryer with V-Corrugated absorber plate is developed. Its experimental performance evaluation in forced convection is carried out for potato chip drying. Various parameters like Hourly Variation of Solar Radiation Intensity, Temperature Distribution Curves inside Solar Dryer, Collector Efficiency Curve, Hourly Mass Loss of Potato Slices and Moisture Removal Comparison with Conventional Open Sun Drying Method are calculated and presented graphically. The peak temperature of the absorber plate reached 69.30C, and the air temperature at the collector outlet recorded the highest value at 57.50C. The average temperature difference of 28.580C is obtained for heat transfer by convection between the modified corrugated absorber plate and the air. An important finding in this experimental investigation was that there is an average 6.50C difference in temperature between the hot air exiting the collector and the air available at the bottom of the lower tray of the drying chamber, which should be reduced by applying means of avoiding heat loss. The highest collector efficiency is calculated as 88.9 % at 2 PM. The lowest efficiency is calculated at 9 AM as 66.8 %. The thermal inertia of the system adds to the collector efficiency in the last 2 hours of the experimentation and hence collector performs better than in the morning hours, though the insolation is nearly the same for the first and last two hours of sunshine. The percentage reduction in drying time was found to be 38.9 % for 50 % moisture removal from potato slices as compared to open sun drying.
Performance Analysis of KNN, Naïve Bayes, and Extreme Learning Machine Techniques on EEG Signals for Detection of Parkinson's Disease
Authors: Haloi Rupjyoti , Chanda Dipankar
Published: October 2024

The application of bio-potentials for diagnosing neurological disorders has become highly effective nowadays. This work focuses on using Electroencephalogram (EEG) to detect Parkinson's disease (PD), a significant neurological disorder. PD is considered the second most common neurological disorder in the world. Being degenerative in nature, it affects the patients progressively. The progression of the severity of this disease can be restricted by a certain limit if its symptoms can be well-treated on time. This work presents a relative analysis of the performances of three machine learning (ML) techniques in detecting PD. These are K-nearest Neighbor (KNN), Naïve Bayes and Extreme Learning Machine (ELM) techniques. Statistical-based features are evaluated from the EEG data signals of normal as well as persons with PD after preprocessing. The features evaluated are then classified using the three techniques. The results of the classifiers are evaluated with the help of some performance parameters such as accuracy, precision, sensitivity, specificity and F1 score. Based on the values of these parameters, the performances of all these techniques are compared. The comparison shows that ELM performs the best, with an accuracy of 98.84% in detecting PD. The reported methodology holds significant clinical relevance. It can offer an early, non-invasive, and objective method for diagnosing, tracking, and managing PD.
Machine Learning-Driven Assessment and Security Enhancement for Electronic Health Record Systems
Authors: Saraswat Birendra Kumar , Varshney Neeraj , Vashist P C
Published: October 2024

The digitalized patient-centric system, the Electronic Health Record (EHR), is a platform where comprehensive health information is stored, managed, and accessed electronically. The primary findings of this study aim to secure sensitive patient data and increase overall system resilience by demonstrating that machine learning can evaluate vulnerabilities and improve the security of Electronic Health Record (EHR) systems. This research examines the prospects of incorporating machine learning-driven assessment tools and safety improvements in EHRs to enhance data protection in the healthcare industry.  The proposed method utilizes the implementation of machine learning classifiers, specifically the XGBoost and LightGBM models. These classifiers are employed to enhance various aspects of the system, such as data protection and security, within the framework of EHRs. The study emphasizes the efficiency of these machine learning classifiers in ensuring that EHR systems are secure enough to deal with any problem that may occur due to threats posed by external factors or hackers. The findings reveal that the XGBoost model always has outstanding performance, with a near-perfect Receiver Operating Characteristic Curve (ROC) having an AUC equal to 1.00, indicating close to perfect accuracy in distinguishing positive from negative cases. Similarly, LightGBM has a perfect ROC curve as well. Therefore, its performance would be considered flawless. Consequently, future developments could lead to sophisticated machine learning models besides those that have already been developed. Improving data storage through encryption and building safer communication protocols should also be considered to make these systems withstand new security problems. Thus, this study contributes to the existing literature on applying technology to safeguard vulnerable medical records while fostering a safe and efficient healthcare ecosystem.
TSOTSALearning at LLMs4OL Tasks A and B : Combining Rules to Large Language Model for Ontology Learning
Authors: Atezong Ymele Carick Appolinaire , Jiomekong Azanzi
Published: October 2024

This paper presents our contribution to the Large Language Model For Ontology Learning (LLMs4OL) challenge hosted by ISWC conference. The challenge involves extracting and classifying various ontological components from multiple datasets. The organizers of the challenge provided us with the train set and the test set. Our goal consists of determining in which conditions foundation models such as BERT can be used for ontologies learning. To achieve this goal, we conducted a series of experiments on various datasets. Initially, GPT-4 was tested on the wordnet dataset, achieving an F1-score of 0.9264. Subsequently, we performed additional experiments on the same dataset using BERT. These experiments demonstrated that by combining BERT with rule-based methods, we achieved an F1-score of 0.9938, surpassing GPT-4 and securing the first place for term typing on the Wordnet dataset.
CARCINOMATOSE MENINGEA DE ADENOCARCINOMA GÁSTRICO DIAGNOSTICADA EM LÍQUOR UTILIZANDO-SE EXPRESSÃO DE CD326 POR CITOMETRIA DE FLUXO
Authors: Watanabe MJL , Souto EX , Amante FAZ , Bacal NS , Bento LC , Fernandes GBP
Published: October 2024

Objetivo: Relato de caso raro de carcinomatose meníngea (CM) por neoplasia gástrica diagnosticada pela análise de líquor (LCR) e expressão de CD326 por citometria de fluxo (CF). Método: Paciente de 56 anos, diagnosticada em 2019 com adenocarcinoma gástrico (AG) pouco diferenciado com células em anel de sinete e suspeição de metástases a distância. Em 2020, devido a sintomas neurológicos, foi feita análise de LCR, a qual evidenciou presença de células neoplásicas; realizada CF: 67,8% de células com ausência de expressão antigênica de CD45 e expressão de CD326, CD71 e ROR-1. Tomografia computadorizada (TC) de crânio: heterogeneidade da medula óssea, múltiplos focos escleróticos; ressonância nuclear magnética (RNM) de crânio: lesões em partes moles e ósseas, contrastação de nervos, sugestivos de acometimento neoplásico secundário, e disseminação leptomeníngea. Focos de hipersinal em FLAIR pós-contraste, compatíveis com carcinomatose meníngea; espaços liquóricos com aumento ventricular desproporcional. Conclusão: A carcinomatose leptomeníngea ocorre em cerca de 5%-8% dos pacientes com câncer; apresenta maior associação com casos de leucemia, adenocarcinoma de mama e pulmão. Em relação ao AG, é rara, com prevalência entre 0,16%-0,69%. Não existe um teste diagnóstico específico, embora a citologia do LCR e RNM sejam muito utilizados. A sensibilidade da RNM de crânio varia entre 65% e 75%, mas em associação com citologia positiva do LCR, aumenta para 91%. A análise do LCR por CF auxilia a confirmação da natureza neoplásica das células com o uso de painéis de anticorpos, incluindo o anti-EpCAM, CD326 que tem sido utilizado em líquidos cavitários e na detecção de células epiteliais, apresentando alta concordância com o estudo imuno-histopatológico (97,6%). Este método é rápido e auxilia o direcionamento do painel imuno-histoquímico, diminuindo o tempo até a conclusão diagnóstica. Referências: 1. Vergoulidou, M. Leptomeningeal carcinomatosis in gastric cancer: a therapeutical challenge. Biomarker Insights. 2017; 12: 1-4.
COMPARAÇÃO ENTRE CITOMETRIA DE FLUXO E CONTADOR HEMATOLÓGICO NA CONTAGEM ABSOLUTA DE LINFÓCITOS
Authors: Dias ICS , Maluf CB , Xavier SG , Vidigal PG
Published: October 2024

Objetivo: O objetivo deste estudo foi comparar dois métodos de contagem absoluta de linfócitos: citometria de fluxo e contagem por analisador hematológico automatizado para ajudar a padronizar e agilizar a avaliação da contagem de leucócitos em pacientes. Método: Sessenta e cinco voluntários foram selecionados, e 5 ml de sangue venoso foram obtidos utilizando o sistema a vácuo contendo EDTA. As amostras foram submetidas à contagem absoluta de linfócitos por dois sistemas distintos. O analisador hematológico Sysmex XN3000 quantificou os leucócitos por fluorescência e dispersão de laser e o citômetro de fluxo FACSCalibur, utilizando tubos Trucount com pellet liofilizado, um anticorpo monoclonal específico. O teste de Shapiro-Wilk foi realizado para verificar a normalidade dos dados. Na correlação entre as variáveis, foi utilizada a correlação de Pearson. O método de Bland-Altman foi utilizado para avaliar a concordância entre as duas metodologias. O nível de significância adotado foi p < 0,05. O erro sistemático total (Bias) adotado foi de 9,19% e o erro total admissível, de 17,60%. Os dados foram processados pelo programa estatístico StataSE 13. Conclusão: Os dois métodos mostraram boa correlação e concordância dentro do erro sistemático e do erro total permitido. Notou-se que a contagem total de linfócitos pelo analisador hematológico foi um pouco menor em comparação com a contagem total de linfócitos realizada pelo citômetro de fluxo. Referências: 1. Grossi V et al. Comparison of methods and TAT assessment: volumetric AQUIOS CL and bead-based FACS CANTO II cytometers. Cytometry. Part B, Clinical Cytometry. 2018; 94(4): 674-78. doi:10.1002/cyto.b.21513. 2. Plebani M et al. Towards harmonization of quality indicators in laboratory medicine. Clin Chem Lab Med. 2013; 51(1): 187-95. doi:10.1515/cclm-2012-0582. 3. Ricos C. Desirable biological variation database specifications westgard. Disponível em: http:www.westgard.com/ biodatabase1.htm. Acesso em: 24 maio 2022.
BIOMARCADORES OXIDATIVOS DE RELEVÂNCIA CLÍNICA EM PACIENTES COM SÍNDROME METABÓLICA
Authors: Chielle EO
Published: October 2024

Objetivo: Indivíduos com síndrome metabólica (SM) possuem aumento de gordura abdominal com liberação exacerbada de adipocinas e resposta oxidativa desregulada. Neles há um aumento significativo de radicais livres nos tecidos, excedendo a capacidade neutralizante dos antioxidantes, observada principalmente pelo aumento das espécies reativas. O objetivo deste estudo foi avaliar níveis de marcadores oxidativos, espécies reativas ao ácido tiobarbitúrico (TBARS), grupos sulfidrila (Grupos SH), capacidade antioxidante total férrica (FRAP) e vitamina C em pacientes com e sem SM, além de verificar suas diferenças. Método: Foi desenvolvido um estudo transversal que incluiu 84 adultos de ambos os sexos (48 sem SM e 36 com SM). Amostras de sangue foram coletadas em jejum e devidamente preparadas e processadas para análise de TBARS, grupos sulfidrila, FRAP e vitamina C. As técnicas foram realizadas conforme protocolos e recomendações dos fabricantes. Conclusão: O grupo com SM apresentou aumento significativo nas concentrações de TBARS, FRAP e ácido úrico (p < 0,05) e redução significativa das concentrações de vitamina C e grupos SH (p < 0,05) quando comparado com o grupo sem SM. Os resultados encontrados são importantes, pois reforçam o desequilíbrio oxidativo de organismos com SM. Esses fatores comprometem a sensibilidade à insulina e podem auxiliar o monitoramento de alterações clínicas e a prevenção de eventos cardiometabólicos futuros em indivíduos com SM. Referências: 1. Sanip Z, Ariffin FD, Al-Tahami BA, Sulaiman WA, Rasool AH. N Obesity indices and metabolic markers are related to hs-CRP and adiponectin levels in overweight and obese females. Obes Res Clin Pract. 2013; 7(4): e315-20. doi: 10.1016/j.orcp.2012.05.002.
APLICABILIDADE DE BUSINESS INTELLIGENCE NO MONITORAMENTO DO TEMPO DE ATENDIMENTO TOTAL DOS PACIENTES ATENDIDOS EM UM LABORATÓRIO CLÍNICO DO LITORAL DE SANTA CATARINA
Authors: Conci TR , Silvestri C , Dall’cortivo G
Published: October 2024

Objetivo: Monitorar o tempo de atendimento nos postos de coleta; mensurar as etapas que o paciente percorre no atendimento; e averiguar a efetividade das ações realizadas e as melhorias nos índices da pesquisa de satisfação. Método: Estudo retrospectivo de dados coletados de janeiro de 2021 a maio de 2022 dos sistemas Shift LIS, Tasy e Weknow. Para o acompanhamento do tempo de atendimento por meio de Business Intelligence (BI), utilizou-se o software Weknow. Os dados do indicador gerados no BI foram extraídos de consultas ao banco de dados do sistema Tasy, através de regras de seleção que trazem informações pertinentes ao problema e possibilitam identificar variáveis que impactam no atendimento ao paciente. Os registros referentes à coleta da amostra ocorrem no sistema Shift e integra ao Tasy, onde ficam armazenados os dados do paciente e seu atendimento. O Weknow, mediante conexão on-line com o Tasy, realiza uma consulta ao banco de dados e atualiza em tempo real as informações no dashboard. As melhorias resultantes do uso do BI foram avaliadas na redução de insatisfações por morosidade em atendimento, geradas na pesquisa de satisfação pelo método NPS. Conclusão: Neste estudo, verificamos que implantar o BI para monitorar o tempo de atendimento total foi primordial para construção de uma cultura analítica no laboratório. Por meio dos dados extraídos de diferentes sistemas, o acompanhamento do indicador acontece de forma rápida e acessível, além de mostrar em tempo real todas as fases do cliente no núcleo de atendimento, focando os esforços da equipe na redução de insatisfação nos serviços e propondo ações corretivas ágeis e assertivas. Referências: 1. Reichheld F, Markey Rob. A pergunta definitiva 2.0: Como as empresas que implementam o net promoter score prosperam em um mundo voltado aos clientes. Alta Books; 2021. 2. Silvestri C. A gestão de tecnologia da informação nos hospitais. Disponível em: https://tiinside.com.br/14/03/2013/a-gestao-de-tecnologia-da-informacao-nos-hospitais/. Acesso em: 30 maio 2022.
MAPEANDO COMPETÊNCIAS: UM ESTUDO SOBRE O PROCESSO SELETIVO E A INFLUÊNCIA DAS COMPETÊNCIAS NA EFETIVIDADE OPERACIONAL EM UM LABORATÓRIO DE ANÁLISES CLÍNICAS DO ESTADO DO PARÁ
Authors: Cohen PB , Costa SCM , Brazão MAB , Brazão FV , Brazão GB
Published: October 2024

Objetivo: Avaliar a performance dos colaboradores e a eficácia do processo de seleção por competências a partir do estudo do índice de avaliação de desempenho dos colaboradores em período de experiência de um laboratório de análises clínicas, enfatizando o setor pré-analítico, por se tratar de processos que requerem maior atenção. Método: Os dados relativos ao mapeamento das competências dos profissionais foram coletados no período de janeiro a março de 2022, pelo setor de gestão de pessoas do laboratório, por meio de formulário com coeficientes de desempenho construídos a partir dos objetivos organizacionais da empresa, tendo como base o perfil de competências dos cargos e o banco de dados inicial dos processos seletivos realizados no período da pesquisa. Conclusão: Foram avaliados 24 colaboradores, e todos obtiveram performance excelente, ou seja, foram evidenciadas as competências previstas no processo seletivo. Destes, 54% atuam no setor pré-analítico, indicando resultado que garante a efetividade operacional no serviço que prestamos. O estudo indicou ainda maior adaptação à cultura organizacional, evidenciando a assertividade das ferramentas de seleção utilizadas nos processos seletivos comprovados pela melhoria do alcance das metas propostas pela gestão da qualidade de suma importância para o alcance dos objetivos organizacionais da empresa. Concluiu-se que o aumento da eficácia no processo de recrutamento e seleção contribui para formação de equipes mais desenvolvidas, trazendo à empresa uma vantagem competitiva, alcançada por meio do seu ativo intelectual, o que ajudará a organização a alcançar seus objetivos mais rapidamente. Frente ao exposto, acredita-se que este estudo contribuirá para a implementação de estratégias gerenciais que promovam melhoria contínua nos processos estratégicos de gestão de pessoas. Referências: 1. Resende E. O livro das competências – desenvolvimento das competências: a melhor autoajuda para pessoas, organizações e sociedade. Rio de Janeiro: Qualitymark; 2000.
PREVALÊNCIA DE UROPATÓGENOS NO PERÍODO DA PANDEMIA DE COVID-19 EM UM LABORATÓRIO DE ANÁLISES CLÍNICAS EM SALVADOR, BAHIA
Authors: Santos LCSC , Pomponet TM , Barreto BO , Araujo CM , Nery LFA , Brandão HLC
Published: October 2024

Objetivo: Avaliar a prevalência da flora microbiana em infecção do trato urinário dos pacientes ambulatoriais atendidos durante o período da pandemia de Covid-19. Métodos: Realizada uma análise retrospectiva dos dados das uroculturas do período de janeiro de 2020 a dezembro de 2021. Conclusão: Foram realizadas 31.747 uroculturas, sendo 26.107 (82,23%) negativas e 5.640 (17,77 %) positivas. Entre essas, houve o predomínio de bactérias com o total de 98,56% e apenas 1,44% com leveduras, sendo 89,13% de bacilos Gram negativos e 10,87% de cocos Gram positivos. O maior número de infecções ocorreu no gênero feminino (87,75%), com 50,56% na faixa etária de 20 a 59 anos e no masculino, com 72,6% acima dos 60 anos. Quanto à etiologia, o predomínio foi a Escherichia coli em 60,53%, seguida da Klebsiella pneumoniae em 14,84% e Proteus mirabilis em 4,59%. Nossos achados apresentaram um perfil semelhante ao do período pré-pandêmico, permanecendo a Escherichia coli como a mais prevalente dentre todos os uropatógenos. Portanto, consideramos que as mudanças de hábitos ocorridas no período da pandemia não foram capazes de alterar a flora bacteriana das infecções urinárias, nem a distribuição da faixa etária dos pacientes ambulatoriais. Referências: 1. Machado AD et al. Prevalência de infecção urinária em um laboratório de análises clínicas da cidade de Jaraguá do Sul, SC, no ano de 2017. RBAC. 2019; 51(3): 213-18. Disponível em: http://www.rbac.org.br/wp-content/uploads/2019/12/RBAC-vol-51-3-2019-ref-821.pdf. Acesso em: 13 maio 2022. 2. Braoios A et al. Infecções do trato urinário em pacientes não hospitalizados: etiologia e padrão de resistência aos antimicrobianos. J Bras Patol Med Lab. 2009. Disponível em: https://doi.org/10.1590/S1676-2444200900060000 3. Acesso em: 26 de maio 2022.
EPIGENOMIC ANALYSIS REVEAL CRITICAL ASSOCIATION BETWEEN METHYLATION STATUS AND CLINICAL PHENOTYPE
Authors: Carvalho GFS , Vieira LL , Wolff BM , Oliveira YG , Almeida VT , Nascimento AM , Kulikowski LD
Published: October 2024

Objective: Copy number variations (CNVs) are DNA fragments deleted or duplicated in relation to a reference genome. Some genome variants, classified as variants with uncertain significance (VUS), do not present a safe conclusion with clinical phenotypes, which makes the diagnostic conclusion difficult. We know that hypermethylation of gene promoter regions often leads to transcriptional silencing, in addition to DNA methylation changes in gene and/or intergenic regions can play a critical role in genomic regulation and stability. With epigenomic investigation, we can gain a more comprehensive understanding of the modulation in gene expression associated with genomic imbalance in different diseases and in different genomic variations. So, our objective is to understand the impact of methylation status associated with genomic imbalances in clinical phenotypes. Method: Our study evaluated, using the Infinium Methylation EPIC Bead Chip platform, 10 DNA samples from patients with intellectual disability and dysmorphic features with CNVs classified as VUS without a definitive clinical diagnosis, in addition to six control samples from participants without clinical conditions. Conclusion: Genomic methylation, associated with genomic structural imbalances, can play a critical role in clinical features and can be used as an important marker for patients with uncertain clinical diagnosis.
IMPORTÂNCIA DOS TESTES MOLECULARES NA DETECÇÃO DE COVID-19 E INFLUENZA DE UM LABORATÓRIO CLÍNICO EM CAMPO GRANDE, MATO GROSSO DO SUL
Authors: Hoff BL , Barreto BO , Araujo C , Abdalla L , Vaz MM
Published: October 2024

Objetivo: Avaliar a prevalência de infecção por Covid-19 e influenza simultaneamente em pacientes atendidos em um laboratório clínico particular de Campo Grande, Mato Grosso do Sul. Método: Foram analisados 1190 pacientes atendidos em janeiro de 2022, com solicitações de exames de influenza e Covid-19, totalizando 1759 resultados. Os resultados foram liberados por meio de ensaio de PCR com amplificação isotérmica e PCR em tempo real multiplex, para detecção e diferenciação de Sars-CoV-2 e vírus influenza A e B. A análise de dados foi realizada em Excel proveniente de sistema informatizado. Conclusão: Após um total de 1190 pacientes atendidos, realizamos 1759 testes pelo método RT-PCR; 1095 foram para Sars-CoV-2, com 575 negativos (52,5%) e 520 positivos (47,5%); 664 foram para influenza A e B – 622 negativos (93,6%) e 42 positivos (6,4%). Dessa amostragem, tivemos ainda 284 pacientes que realizaram tanto o teste para Sars-CoV-2 quanto para influenza simultaneamente, com apenas duas coinfecções. Foram notificados 422.264 casos de Covid-19 e 801 de influenza na cidade de Campo Grande-MS no mesmo período, confirmando assim a prevalência de Covid-19 positivo encontrado no laboratório. Mesmo com resultados de Sars-CoV-2 positivo, significativamente superior em relação à influenza A e B, a preocupação estava na alta taxa de mortalidade da influenza (18%) em relação à Covid-19 (2,3%). Nota-se, portanto, a importância dos testes moleculares na diferenciação dessas infecções, com identificação do patógeno e, consequentemente, manejo clínico, detectando também possíveis coinfecções que aumentam os riscos de quadros graves, internações e óbito. Referências: 1. Atualização epidemiológica: influenza no contexto da pandemia de Covid-19 28/12/21. Brasília, DF: OPAS/OMS; 2022. 2. Castro LFSO, et al. Influenza e Sars-CoV-2: distinção clínica e imunopatológica no decorrer da pandemia da Covid-19. Rev Eletrônica Acervo Saúde. 2022; 15(3).
ASPECTOS EPIDEMIOLÓGICOS E CLÍNICOS DE PROCESSOS INFECIOSOS CAUSADOS POR ESPÉCIES DE MYCOBACTERIUM
Authors: Bastos NA , Dias VC , Bastos MPM , Bastos VQA , Bastos RV , Bastos LQA , Hipolito JR
Published: October 2024

Objetivo: Efetuar análise temporal (2016 a 2021) de aspectos epidemiológicos e clínicos de processos infecciosos causados por espécies de Mycobacterium, em pacientes atendidos em um laboratório clínico de Juiz de Fora, Minas Gerais. Método: Dados como idade, sexo e origem dos participantes, bem como etiologia da infecção, foram obtidos a partir de consulta em prontuário eletrônico. A etiologia foi determinada a partir de cultura utilizando ágar Lowenstein Jensen, com posterior caracterização da espécie de Mycobacterium por meio de reação em cadeia da polimerase (PCR). Conclusão: Devido a sua presença constante no meio ambiente, é importante distinguir a colonização transitória em relação à doença ativa após a constatação da presença de MNT. Este estudo alerta para o potencial de contágio das micobactérias na comunidade, especialmente entre os indivíduos jovens. Outros estudos envolvendo o significado clínico do aumento da taxa de isolamento de MNT de amostras clínicas para melhor compreender as mudanças na epidemiologia dessas bactérias e seu impacto na prática clínica são necessários. Referências: 1. Al-Mutairi NM, Ahmad S, Mokaddas EM. Molecular characterization of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) isolates identifies local transmission of infection in Kuwait, a country with a low incidence of TB and MDR-TB. Eur J Med Res. 2019; 24(1): 38. doi: 10.1186/s40001-019-0397-2. Erratum in: Eur J Med Res. 2020 Apr 21;25(1):14. PMID: 31806020; PMCID: PMC6894303. 2. López-Roa P, Aznar E, Cacho J et al. Epidemiologyof non-Tuberculous mycobacteria isolated from clinical specimens in Madrid, Spain, from 2013 to 2017. Eur J Clin Microbiol Infect Dis. 2020; 39(6): 1089-94. doi: 10.1007/s10096-020-03826-7. Epub 2020 Jan 24. PMID: 31980987.
METHYLATION AND BIOLOGICAL AGE ANALYSIS IN ADHD PATIENTS
Authors: Kulikowski LD , Carvalho G , Oliveira YG , Costa TVMM , Vieira LL , Almeida VT , Nascimento A
Published: October 2024

Objective: Attention deficit/hyperactivity disorder (ADHD) is a common behavioral syndrome that begins in childhood and affects 3.4% of children worldwide. Due to its etiological complexity, there are no consistent biomarkers for ADHD, however the high heritability that the disorder presents indicates an influence of genetic/ epigenetic factors. The main epigenetic mechanisms is DNA methylation, a process with an important role in gene expression and in many biological pathways. Methylation status can be used as a biomarker for clinical conditions, such as cancer and neuropsychiatric diseases with specific epi-signatures, and it is used as a marker of biological senescence by several studies to determine biological age (DNAmAge). Thus, our study sought to identify epi-signatures biomarkers in 29 children clinically diagnosed with ADHD. Method: We using the Infinium Human Methylation 450 platform BeadChip (Illumina) and a pipeline of bioinformatics analysis in R programming language.To obtain biological ages through epigenetic marker. Conclusion: The results highlight the interaction of energy metabolism and oxidative stress pathways in ADHD patients detected by differential methylation patterns. We were able to identify a marginal association between the DNAmAge and ADHD. Thus, we present new methylation biomarkers in ADHD patients and propose that further multiethnic studies with larger cohorts should be done to demonstrate a definitive association between ADHD and these methylation biomarkers. References: 1. Schuch V, Utsumi DA, Costa TVMM, Kulikowski LD, Muszkat M. Attention déficit hyperactivity disorder in the light of the epigenetic paradigm. Front Psychiatry. 2015; 6: 126. 2. Pelegí-Sisó D, de Prado P, Ronkainen J, Bustamante M, González JR. Methylclock: a bioconductor package to estimate DNA methylation age. Bioinformatics. 2021; 37(12): 1759-60.