bluuebunny/crossref_metadata_2025 huggingface dataset:
Leveraging Guided Backpropagation to Select Convolutional Neural Networks for Plant Classification
Authors: Mostafa Sakib , Mondal Debajyoti , Beck Michael A. , Bidinosti Christopher P. , Henry Christopher J. , Stavness Ian
Published: May 2022

The development of state-of-the-art convolutional neural networks (CNN) has allowed researchers to perform plant classification tasks previously thought impossible and rely on human judgment. Researchers often develop complex CNN models to achieve better performances, introducing over-parameterization and forcing the model to overfit on a training dataset. The most popular process for evaluating overfitting in a deep learning model is using accuracy and loss curves. Train and loss curves may help understand the performance of a model but do not provide guidance on how the model could be modified to attain better performance. In this article, we analyzed the relation between the features learned by a model and its capacity and showed that a model with higher representational capacity might learn many subtle features that may negatively affect its performance. Next, we showed that the shallow layers of a deep learning model learn more diverse features than the ones learned by the deeper layers. Finally, we propose SSIM cut curve, a new way to select the depth of a CNN model by using the pairwise similarity matrix between the visualization of the features learned at different depths by using Guided Backpropagation. We showed that our proposed method could potentially pave a new way to select a better CNN model.
The Ambiguous Impact of Performance Measurement on Service Quality
Authors: Tøssebro Jan , Mjøen Odd Morten , Bruteig Rebekka
Published: May 2022

Background

Performance measurement is growing in importance as a management tool in services for disabled people.

Aim

The aim of this article is to add to the existing literature by exploring (a) the motivation for the introduction of such measurements, (b) the reasoning behind the choice of current indicators, and (c) the impact of performance measurements on service delivery.

Methods

(1) A study of documents (national and, if available, also local) on the motivation for, choice of, and implementation of quality measurements, and (2) interviews with top and middle managers in community services for people with intellectual disabilities or mental health difficulties.

Results

A varied set of motivations have been identified, including the intention to introduce a more facts-based and transparent governance, the need for information that supports the management of scarce resources, and as a tool in the development of service quality for users. The motivation appears to be dependent on level of government, and the attitude among service unit managers tends to be ambivalent; they want performance measurements but cannot see how to measure the important aspects of service quality. The choice of actual indicators is subject to a process bias; that is, one measures what is easily available in administrative systems. The results concerning impact on services are less clear and also context dependent. We have identified usage in the search for cost-cutting possibilities, defense against critique, and that reporting runs the risk of reinforcing routinization of services.

Discussion

The possible impact on services is discussed. Layers of ambiguity are outlined, as measurements can be tools both for quality development and in the defense of current services against “unrealistic demands” from the media or stakeholders. The measurements tend to be used more as sources of governance information than tools for quality development.

Conclusion

The impact of quality measurement is rather ambiguous. On the one hand, it functions as a tool for budget control, whereas on the other hand, unit managers call for better measurement of user outcomes and expect that such measurement can balance the current preoccupation with input indicators, such as expenditures.
Facilitating Personal Transformation for Sustainability: A Learning Program on the Sustainable Development Goals, Combining a Card Game and a Self-Reflective Questionnaire
Authors: Kitamura Kenji , Ito Koji
Published: May 2022

Sustainable Development Goals (SDGs) require transformations at all levels, from global to local, to be realized. Underlying all of these levels is the personal level, where any transformation begins. Before individual persons change their behavior for sustainability, they will need to not only understand the SDGs, but also regard them as a matter which directly concerns them. A process involving “personalizing the SDGs” needs to exist between initial awareness and subsequent action. However, identifying and instituting the means by which this can be realized is not easy. In this study, we designed a learning program that combined an existing SDGs game with an original self-reflective questionnaire, and implemented it in Ishikawa Prefecture, Japan. The program allowed participants (n = 289) to first play the game, which takes the form of a simulation of the world in the run up to 2030, and then carry out individual and/or group work to consider their individual, specific relationships with the global agenda. The questionnaire also provided each participant with a means to reflect on their initial perceptions and how they changed following this learning. The answers on the questionnaire indicated that the program aided the participants in raising their perceived levels of relationships with, and the personal significance of, the SDGs, while having fun at the same time. Personal relationships with the SDGs were expressed more concretely when local contexts and topics covered in other learning programs were tied in with the SDGs.
Relationships Between Resilience, Mental Well-Being, and COVID-19 Worries in Collegiate Student-Athletes
Authors: Watts Cade J. , Hilliard Robert C. , Graupensperger Scott
Published: May 2022

The onset of the COVID-19 pandemic was associated with robust declines in well-being for collegiate student-athletes. Worries about COVID-19 have frequently been associated with worsening well-being; therefore, it is important to examine protective factors against well-being decrements. Resilience, one's ability to respond to stress and adversity, may be one such factor. Despite this possible influence, resilience has not yet been studied in student-athletes in this context as the pandemic has progressed. Therefore, the purpose of this study was to examine the moderating role of resilience on the relationship between COVID-19 worries and well-being. In this cross-sectional design, National Collegiate Athletic Association Division III athletes (N = 91) at one university completed surveys on COVID-19 worries, resilience, and well-being between February and March 2021. All competitions had been postponed until the Spring 2021 semester. The findings revealed a negative correlation between COVID-19 worries and well-being (r = −0.21, p = 0.05) and a positive correlation between resilience and well-being (r = 0.44, p < 0.001). Additionally, multiple regression and simple slopes analyses showed that individuals with higher resilience endorsed greater scores of well-being, even when COVID-19 worries increased (β = 0.38, p = 0.02). In conclusion, our results suggest that resilience had a moderating effect on the relationship between COVID-19 worries and well-being.
Preliminary Characterization of Structural and Rheological Behavior of the Quinoa Hyperprotein-Defatted Flour
Authors: Ortiz-Gómez Vicente , Nieto-Calvache Jhon Edinson , Roa-Acosta Diego Fernando , Solanilla-Duque Jose Fernando , Bravo-Gómez Jesús Eduardo
Published: May 2022

Protein functional properties are related to physical and chemical parameters that influence protein behavior in food systems during processing, storage and consumption. The structural and rheological properties of three quinoa hyperprotein flours (without defatting, WD, chemically defatted, CD, and mechanically defatted, MD) were evaluated. The values of the fluidity index (n) were significantly different (p < 0.05), which was associated with changes in protein or starch structures due to solvent treatments or heating of the flour during pressing. In addition, a strong dependence of the consistency index (k) on the shear rate was observed. For dispersions with a concentration of 12% (w/v), CD and WD had a significantly lower setback value than MD. The viscosity peak was affected by the presence of lipid molecules. Greater changes were evident in the β-sheet (1,610 and 1,625 cm−1) and β-spin (1,685 and 1,695 cm−1) structures. The changes identified in these structures were associated with the defatting treatment. Consequently, the intensity ratio 2,920/1,633 cm−1 was more sensitive to changes in the fat content of the flours. It was shown that defatting conditions increase the protein adsorption kinetics and that the viscoelastic properties of the protein increase when the flour has a lower fat content. Hyperprotein quinoa flour could be used to improve the protein content of products such as snacks, pastas, ice cream, bakery products, meat extenders, among others, due to its foaming, gelling or emulsifying capacity. The objective of this work was to study the effect of two types of defatting of hyperprotein quinoa flour on its structural and rheological properties.
Purified PTEN-Long Induces Liver Cancer Cells to Undergo Autophagy and Apoptosis
Authors: Tan Lin , Xu Zeping , Mao Qiqi , Zhou Shaocheng , Zhu Jie , Zhang Xie , Li Hong
Published: May 2022

Background

PTEN-Long is a translational variant of phosphatase and tensin homolog deleted on chromosome 10 (PTEN). This tumor suppressor is frequently lost or mutated and even it has been shown as the determinant in several human tumors. Therefore, we will determine the significant roles of PTEN-Long in the development of liver cancer.

Methods

In the present study, we characterized the antitumor effects of PTEN-Long and PTEN in proliferation, migration of HepG2 cells, apoptosis and autophagy in liver cancer cells. To extends, we have also measured the effects of purified PTEN and PTEN-Long in the above index of HepG2 cells.

Results

PTEN and PTEN-Long were ectopic-expressed in HepG2 cells, and their phenotypic effects were recorded. As expected, there was less expression of PTEN-Long and PTEN in liver cancer samples than in paired normal tissues. Ectopic expression of PTEN-Long or PTEN significantly decreased the proliferation and migration of HepG2 cells and increased apoptosis. PTEN ectopic-expression increased the number of GFP-/RFP+-LC3 puncta and levels of beclin-1 and LC3BII/LC3BI, suggesting autophagy induction. Purified PTEN-Long freely entered cells, decreased proliferation, and increased autophagy and apoptosis, while purified PTEN did not.

Conclusions

Our results identify an antitumor function of purified PTEN-Long and suggest its potential utility for liver cancer treatment.
Impact of Time of Surgery on the Outcome after Surgical Stabilization of Rib Fractures in Severely Injured Patients with Severe Chest Trauma—A Matched-Pairs Analysis of the German Trauma Registry
Authors: Becker L. , Schulz-Drost S. , Spering C. , Franke A. , Dudda M. , Kamp O. , Lefering R. , Matthes G. , Bieler D. ,   on behalf of the Committee on Emergency Medicine, Intensive Care and Trauma Management (Sektion NIS) of the German Trauma Society (DGU)
Published: May 2022

Purpose

In severely injured patients with multiple rib fractures, the beneficial effect of surgical stabilization is still unknown. The existing literature shows divergent results, and the indication and especially the right timing of an operation are the subject of a broad discussion. The aim of this study was to determine the influence of the time point of surgical stabilization of rib fractures (SSRF) on the outcome in a multicenter database with special regard to the duration of ventilation, intensive care, and overall hospital stay.

Methods

Data from the TraumaRegister DGU collected between 2010 and 2019 were used to evaluate patients above 16 years of age with severe rib fractures [Abbreviated Injury Score (AIS)  ≥ 3] who received an SSRF in a matched-pairs analysis. In this matched-pairs analysis, we compared the effects of an early SSRF within 48 h after initial trauma vs. late SSRF 3–10 days after trauma.

Results

After the selection process, we were able to find 142 matched pairs for further evaluation. Early SSRF was associated with a significantly shorter length of stay in the intensive care unit (16.2 days vs. 12.7 days, p = 0.020), and the overall hospital stay (28.5 days vs. 23.4 days, p = 0.005) was significantly longer in the group with late SSRF. Concerning the days on mechanical ventilation, we were able to demonstrate a trend for an approximately 1.5 day shorter ventilation time for patients after early SSRF, although this difference was not statistically significant (p = 0.226).

Conclusions

We were able to determine the significant beneficial effects of early SSRF resulting in a shorter intensive care unit stay and a shorter length of stay in hospital and additionally a trend to a shorter time on mechanical ventilation.
RETRACTED: Longitude Variation of the microRNA-497/FGF-23 Axis during Treatment and Its Linkage with Neoadjuvant/Adjuvant Trastuzumab-Induced Cardiotoxicity in HER2-Positive Breast Cancer Patients
Authors: Liu Hui , Hu Xiaoyan , Wang Lingyun , Du Tao , Feng Jing , Li Ming , Liu Lei , Liu Xiaofang
Published: May 2022

Purpose

MicroRNA-497 (miR-497) is previously reported to target fibroblast growth factor 23 (FGF-23) and regulates cardiac injury, while their value in predicting drug-induced cardiotoxicity is not reported. Thus, the current study aimed to investigate the correlation of miR-497/FGF-23 with neoadjuvant/adjuvant trastuzumab-induced cardiotoxicity in human epidermal growth factor receptor 2 (HER2)-positive breast cancer patients.

Methods

A total of 97 HER2-positive surgical breast cancer patients who received neoadjuvant/adjuvant trastuzumab contained regimens were enrolled; then, their peripheral blood mononuclear cells (PBMC) and serum were collected at baseline, after neoadjuvant treatment, at 3 months (M3), 6 months (M6), 9 months (M9), and 12 months (M12) after surgery. The PBMC was used for miR-497 measurements, and the serum was used for FGF-23 measurements. The cardiotoxicity events and incidence were recorded.

Results

A total of 24 (24.7%) patients occurred cardiotoxicity during the treatment period. MiR-497 decreased from baseline (median: 0.955) to M12 after surgery (median: 0.602) (p < 0.001), while FGF-23 increased from baseline (median: 0.390 ng/mL) to M12 after surgery (median: 0.566 ng/mL) (p < 0.001); besides, the miR-497/FGF-23 axis greatly reduced from baseline (median: 2.545) to M12 after surgery (median: 1.222) (p < 0.001). At most time points, miR-497 was negatively related to FGF-23 (all p < 0.05). Notably, the miR-497/FGF-23 axis at all time points (including baseline, postneoadjuvant treatment, M3, M6, M9, and M12) was related to a lower risk of cardiotoxicity (all p < 0.05). Furthermore, the miR-497/FGF-23 axis was also positively correlated with the left ventricular ejection fraction (LVEF) at all time points (all p < 0.01).

Conclusion

The MiR-497/FGF-23 axis serves as a potential indicator predicting trastuzumab-induced cardiotoxicity in HER2-positive breast cancer patients.
Prediction of Long-Term Survival Outcome by Lymph Node Ratio in Patients of Parotid Gland Cancer: A Retrospective study
Authors: Jiang Wen-Mei , Xu Jian-Feng , Chen Jun , Li Guo-Li , Gao Yun-Fei , Zhang Quan , Chen Yan-Feng
Published: May 2022

Background

Lymph node ratio (LNR) has been reported to reliably predict cancer-specific survival (CSS) in parotid gland cancer (PGC). Our study was designed to validate the significance of LNR in patients with PGC.

Methods

Patients diagnosed with stage I–IV PGC were enrolled from Surveillance Epidemiology and End Results database (SEER, N = 3529), which is the training group, and Sun Yat-sen University Cancer Center database (SYSUCC, N = 99), the validation group. We used X-tile software to choose the optimal cutoff value of LNR; then, univariable and multivariable analyses were performed, assessing the association between LNR and CSS.

Results

The optimal cutoff value of LNR was 0.32 by X-tile based on 3529 patients from SEER. Cox proportional hazard regression analysis revealed better CSS for patients with LNR ≤ 0.32 (adjusted hazard ratio [HR] 1.612, 95% confidence interval [95% CI] 1.286–2.019; p < 0.001) compared with patients with LNR > 0.32 in SEER. In the SYSUCC cohort, patients with LNR ≤ 0.32 also had better CSS over patients with LNR > 0.32 (p < 0.001). In N2 and N3 stage groups, patients with LNR ≤ 0.32 had superior CSS outcomes over those with the LNR > 0.32 group, but this benefit was absent in the N1 stage group.

Conclusions

In conclusion, the lymph node ratio turned out to be an independent prognostic factor for cancer-specific survival of PGC in this study. This valuable information could help clinicians to evaluate the prognosis of PGC and suggest that adequate lymph node dissection is necessary.
Role of Environmental Toxicants on Neurodegenerative Disorders
Authors: Nabi Masarat , Tabassum Nahida
Published: May 2022

Neurodegeneration leads to the loss of structural and functioning components of neurons over time. Various studies have related neurodegeneration to a number of degenerative disorders. Neurological repercussions of neurodegeneration can have severe impacts on the physical and mental health of patients. In the recent past, various neurodegenerative ailments such as Alzheimer’s and Parkinson’s illnesses have received global consideration owing to their global occurrence. Environmental attributes have been regarded as the main contributors to neural dysfunction-related disorders. The majority of neurological diseases are mainly related to prenatal and postnatal exposure to industrially produced environmental toxins. Some neurotoxic metals, like lead (Pb), aluminium (Al), Mercury (Hg), manganese (Mn), cadmium (Cd), and arsenic (As), and also pesticides and metal-based nanoparticles, have been implicated in Parkinson’s and Alzheimer’s disease. The contaminants are known for their ability to produce senile or amyloid plaques and neurofibrillary tangles (NFTs), which are the key features of these neurological dysfunctions. Besides, solvent exposure is also a significant contributor to neurological diseases. This study recapitulates the role of environmental neurotoxins on neurodegeneration with special emphasis on major neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease.
Asymptomatic Anticoagulant Rodenticide Exposure in Dogs and Cats—A French and Belgian Rural and Urban Areas Study
Authors: Mahjoub Tarek , Krafft Emilie , Garnier Léa , Mignard Amélie , Hugnet Christophe , Lefebvre Sébastien , Fourel Isabelle , Benoit Etienne , Lattard Virginie
Published: May 2022

Anticoagulant rodenticides (ARs) are important tools for controlling rodent pests, but they also pose a health threat to non-target species. ARs are one of the most common causes of pet poisoning. However, exposure of domestic animals to subclinical doses of ARs is poorly documented. To study the random exposure of dogs and cats to ARs, feces from animals showing no clinical signs of rodenticide poisoning were collected from a network of French and Belgian veterinarians. We analyzed fresh feces from 304 dogs and 289 cats by liquid chromatography-tandem mass spectrometry. This study showed a limited prevalence of AR exposure in dogs and cats of 2.6 and 4.5% respectively. In both species, access to the outdoors is a risk factor for ARs exposure. In contrast, the sex of the animals did not affect the ARs exposure status. The observation of the ratio of cis and trans isomers suggested primary exposure in dogs, but also in some cats. While primary exposure in dogs appears to be related to the use of ARs as plant protection products, primary exposure in cats may be malicious, as warfarin, an anticoagulant formerly used as a rodenticide and now used only in humans, was found in 4 of 13 exposed cats. Secondary exposure may also occur in cats.Our study showed reduced exposure in dogs and cats, compared to wildlife, which often has high exposure, especially in areas where rodent control is important.
A Retrospective Study on Mandibular Reconstruction Following Excision of Canine Acanthomatous Ameloblastoma
Authors: Tsugawa Anson J. , Arzi Boaz , Vapniarsky Natalia , Verstraete Frank J. M.
Published: May 2022

The successful excision of a locally invasive tumor such as canine acanthomatous ameloblastoma (CAA) typically results in a mandibular contour-derforming, critical-size defect that alters the jaw kinematics, and may affect the patient's quality of life. In this case series, we describe our experience using the regenerative approach of a titanium locking plate and compression resistant matrix infused with rhBMP-2 for the immediate or delayed reconstruction following mandibulectomy for the excision of mandibular CAA in 11 dogs. Surgical planning included computed tomography (CT), with and without contrast, in all cases, and 3D-printed models in four cases. Tumor-free surgical margins were achieved in all dogs. Clinical and diagnostic imaging follow-up (mean, 23.1 months) were performed in-person (11 cases) and with CT/cone-beam computed tomography in most cases, with standard radiography (3 cases) and telemedicine being utilized in 5 cases. At 2 weeks postoperatively, hard tissue was palpable at the defect. Follow-up imaging at 1 month postoperatively revealed evidence of bridging new bone with a heterogeneous appearance, that remodeled over 3–6 months to bone of a similar size, shape and trabecular pattern as native bone. Histological evaluation of regenerated bone was available in two cases, and was supportive of our clinical and imaging findings of normal remodeled bone. Clinically, all dogs returned to a normal lifestyle, rapidly resumed eating and drinking, and exhibited normal occlusion. Complications included wound dehiscence in one dog and self-limiting exuberant bone formation in two dogs. Tumor regrowth, failure of the implant or fracture of the regenerated bone were not observed. We conclude that the mandibular reconstruction using a regenerative approach is safe, feasible, and results in restoration of mandibular contour in dogs following segmental and bilateral rostral mandibulectomy for benign but invasive oral tumors such as CAA.
A Cross-National Comparison on Life Expectancy of Non-Hispanic White Americans
Authors: Xie Lingling , Xiang Defang , He Haijun , Zhai Tiemin , Mao Zongfu , Liang Xiaohui
Published: May 2022

Objectives: Taking the life expectancy (LE) of Non-Hispanic White (NHW) Americans as an example to provide potential references for improving LE globally.

Methods: We collected complete data from the United States (US) CDC, Office for National Statistics in the United Kingdom (UK), and the OECD publications, and described LE changes of NHW Americans by cross-national comparison and Arriaga’s method.

Results: LE of NHW Americans was not as optimistic as European countries from 2006 to 2018. The LE annual average growth rate was 0.04% for NHW Americans, 0.19% for the UK population, and the median of 25 countries was 0.24%. Compared with the other age groups, the age group 30–34 revealed an inferior impact on the LE of NHW people, of which accidents and intentional self-harm were likely to be the top two direct causes.

Conclusion: Finding out the direct causes that affect the LE growth in different age groups is conducive to making a targeted intervention or solving the LE growth bottleneck.
COVID-19 Impacts Across Multiple Life Domains of Vulnerable Socio-Demographic Groups Including Migrants: A Descriptive Cross-Sectional Study
Authors: Chilunga Felix P. , Coyer Liza , Collard Didier , Leenstra Tjalling , Galenkamp Henrike , Agyemang Charles , Prins Maria , Stronks Karien
Published: May 2022

Objectives: We assessed the impacts of COVID-19 on multiple life domains across socio-demographic groups in Netherlands.

Methods: After the first COVID-19 wave, we distributed online questionnaires among 13,031 participants of the multi-ethnic HELIUS cohort. Questionnaires contained questions on changes in income status, healthy behaviors, mental health, and access to non-COVID-19 health care. We then calculated differences in adjusted proportions of participants that reported negative changes across multiple life domains by migration background, age, sex, education, and occupation.

Results: 4,450 individuals (35%) responded, of which 4,294 were included. Older populations and men seemed to be less vulnerable to negative changes in multiple life domains during the COVID-19 pandemic as compared to the pre-pandemic period, while populations with a migration background and lower education/occupation groups seemed to be more vulnerable to negative changes.

Conclusion: Not all populations vulnerable to SARS-CoV-2 infection and mortality are also more vulnerable to COVID-19 impacts across multiple other life domains. Targeted interventions are needed in socio-demographic groups that are most impacted by COVID-19 in various life domains to prevent a further increase of their already increased risk of chronic diseases after the pandemic.
Women Who Sell Sex in Eastern and Southern Africa: A Scoping Review of Non-Barrier Contraception, Pregnancy and Abortion
Authors: Macleod Catriona Ida , Reynolds John Hunter , Delate Richard
Published: May 2022

Objectives: There is a need to hone reproductive health (RH) services for women who sell sex (WSS). The aim of this review was to collate findings on non-barrier contraception, pregnancies, and abortion amongst WSS in Eastern and Southern African (ESA).

Methods: A scoping review methodology was employed. Inclusion criteria were: 1) empirical papers from 2) ESA, 3) published since 2010, and 4) addressing WSS in relation to 5) the identified RH issues.

Results: Reports of rates of non-barrier contraceptive usage varied from 15% to 76%, of unintended pregnancy from 24% to 91%, and of abortion from 11% to 48%. Cross-cutting factors were alcohol use, violence, health systems problems, and socio-economic issues. Pregnancy desire was associated with having a non-paying partner. Barriers to accessing, and delaying, antenatal care were reported as common. Targeted programmes were reported as promoting RH amongst WSS.

Conclusion: Programmes should be contextually relevant, based on local patterns, individual, interpersonal and systemic barriers. Targeted approaches should be implemented in conjunction with improvement of public health services. Linked HIV and RH services, and community empowerment approaches are recommended.
Whole-Exome Sequencing Could Distinguish Primary Pulmonary Squamous Cell Carcinoma From Lung Metastases in Individuals With Cervical Squamous Cell Carcinoma
Authors: Li Lihong , Song Qianqian , Cao Dandan , Jiao Yuchen , Yuan Guangwen , Song Yan
Published: May 2022

Aims: Metastatic cervical carcinoma is hard to cure using traditional treatment and new therapeutic approaches are needed. However, the process of clonal evolution and the molecular alterations that contribute to tumor progression from primary to metastatic carcinoma remain unclear. It is currently difficult to distinguish between the primary pulmonary squamous cell carcinoma (PPSCC) and metastatic cervical squamous cell carcinoma (CSCC).

Methods: Paired primary CSCC and lung/lymph nodes metastatic lesions from eight patients were analyzed by whole-exome sequencing (WES). WES data of matched specimens and normal samples were aligned to the human reference genome and analyzed to identify somatic mutations in primary and metastatic lesions.

Results: A total of 1,254 somatic variants were identified. All the primary lesions and metastatic lesions shared mutations, the percentage of shared mutations between primary lesions and corresponding metastatic lesions varied significantly, ranging from 6% to 70%. In other words, all the metastatic lesions are clonally related to primary lesions, confirming WES could prove they are metastatic from the cervix but not PPSCC. We tried to apply a gene panel to help distinguish PPSCC and metastatic CSCC but failed because the mutations were widely distributed in CSCC. Interestingly, lymph nodes metastasis (LNM) harbored fewer cancer driver mutations than primary CSCC specimens with a significant difference. Besides this, there was no significant difference in somatic mutations and copy number variation (CNV) between primary and metastatic CSCC.

Conclusion: Our data demonstrate that WES is an additional helpful tool in distinguishing PPSCC and metastatic CSCC, especially for certain difficult cases.
SentenceLDA- and ConNetClus-Based Heterogeneous Academic Network Analysis for Publication Ranking
Authors: Zhang Jinsong , Jin Bao , Sha Junyi , Chen Yan , Zhang Yijin
Published: May 2022

Scientific papers published in journals or conferences, also considered academic publications, are the manifestation of scientific research achievements. Lots of scientific papers published in digital form bring new challenges for academic evaluation and information retrieval. Therefore, research on the ranking method of scientific papers is significant for the management and evaluation of academic resources. In this paper, we first identify internal and external factors for evaluating scientific papers and propose a publication ranking method based on an analysis of a heterogeneous academic network. We use four types of metadata (i.e., author, venue (journal or conference), topic, and title) as vertexes for creating the network; in there, the topics are trained by the SentenceLDA algorithm with the metadata of the abstract. We then use the Gibbs sampling method to create a heterogeneous academic network and apply the ConNetClus algorithm to calculate the probability value of publication ranking. To evaluate the significance of the method proposed in this paper, we compare the ranking results with BM25, PageRank, etc., and homogeneous networks in MAP and NDCG. As shown in our evaluation results, the performance of the method we propose in this paper is better than other baselines for ranking publications.
Institutional Change and Organisational Resistance to Gender Equality in Higher Education: An Irish Case Study
Authors: Hodgins Margaret , O’Connor Pat , Buckley Lucy-Ann
Published: May 2022

Attempts to transform the gendered structures and cultures of higher education institutions have had limited success. This article focuses on one Irish university (pseudonym University A) where gender inequality was a major concern culminating in high-profile litigation. Using a feminist institutional approach, it asks: (1) What changes and interventions were introduced in the context of a favorable national policy environment and local grassroots support? and (2) how were these interventions perceived by staff? The methodology draws, firstly on the authors’ personal knowledge of the local context; secondly, on an analysis of University A’s key gender equality-related actions and documents; and thirdly, on a thematic analysis of qualitative data from 129 respondents in an online survey. Positive changes are identified, as is institutional resistance, reflected in low ambitions and focus on individualistic solutions. In the qualitative data, resistance involving denial; assertions that the problem is solved; the importance of meritocracy, and a focus on “fixing the women” (and the men) were identified. Acceptance of gender equality as an organisational issue was reflected in criticism of the interventions as tokenistic window dressing; not impacting on the culture and “not going far enough”. The implications for effectively addressing gender inequality are discussed.
Influence of Seed Quality Stimulation in “Khao Dawk Mali 105” Rough Rice during the Deterioration Period Using an Automatic Soaking and Germination Accelerator Unit and Infrared Radiation Treatment
Authors: Vipattanaporn Chanat , Chiawchanwattana Cherdpong , Laohavanich Juckamas , Yangyuen Suphan
Published: May 2022

This study aimed to improve the seed quality during the deterioration period of rough rice (Oryza sativa L.), cultivar ‘Khoa Dawk Mali 105’ (KDML 105), using an automatic soaking and germination accelerator unit (ASGA) together with stimulation via infrared radiation treatment (IRT) to stimulate seed quality (germination rate and γ-aminobutyric acid (GABA) content). This study used a general full factorial design, and the independent variables were the storage period (10, 11 and 12 months), methods of germinated rough rice preparation (conventional method (CM) and an automatic soaking and germination accelerator unit (ASGA)), and stimulation with IRT. The initial grain moisture content did not exceed 14% (wet basis (wb)). The germination rate of the rough rice by CM and ASGA with stimulation with IRT was significantly higher than non-stimulated rice, by 6.56 and 8.11%, respectively, in each storage period. The GABA contents of the germinated rough rice using CM and ASGA stimulated with IRT were significantly higher than ungerminated rough rice, by 19.52 and 21.24% (10 months), respectively; 16.36 and 23.58% (11 months), respectively; and 69.88 and 67.69% (12 months), respectively.
Sensitivities of Physical and Chemical Attributes of Soil Quality to Different Tillage Management
Authors: Zhao Hongxiang , Wu Li , Zhu Shuwei , Sun Hongchang , Xu Cailong , Fu Jindong , Ning Tangyuan
Published: May 2022

Tillage management is a direct factor in affecting soil quality, which is a key factor in sustainable agriculture. However soil quality evaluation needs significant manpower, material resources and time. To explore the sensitive indicators of soil quality affected by tillage management, eight soil physical and chemical properties under three tillage managements, including plow tillage, subsoiling tillage and rotary tillage, were determined under a long-term experiment in North China Plain. The results showed that subsoiling tillage management had the highest soil organic carbon and total nitrogen in the 0–20 cm layer and the lowest soil bulk density in the 30–40 cm layer. Rotary tillage management had the highest soil water content in the 0–40 cm layer. Meanwhile, compared to 2002, the soil organic carbon, total nitrogen and soil bulk density had varied greatly in 2012, but there was no significant difference between 2012 and 2018. However, other property concentrations tended to increase in 2002, 2012 and 2018. In addition, there was a significant linear relationship between soil quality index and grain yield. Subsoiling tillage management had the highest soil quality index and gain yield both in 2012 and 2018. The soil quality can be evaluated through the sensitive indicator of soil organic carbon, total nitrogen, soil bulk density, total phosphorus and soil water content, which provides a scientific basis for selecting reasonable tillage management and evaluating soil quality in this agricultural production area or other similar areas.
Can Olive Pruning Forms Influence the Olive Rhizosphere? The Root Microbiota and the Rhizosphere Properties in the Alto Ricaurte (Colombia)
Authors: García-Molano José Francisco , Cuervo-Bejarano William Javier , Rodolfi Margherita , Jaramillo-García Luz Stella , Ganino Tommaso
Published: May 2022

(1) Background: Olive in Colombia is not a traditional crop, but in the Andean Region, ancient olive trees are widespread. The area is characterized by a climate condition with a high intensity of UV rays and meteoric events that negatively affect the olive grown. In this work, changes in the soil of olive trees subjected to different pruning will be established. (2) Methods: Olive trees of 2-years-old were cultivated in Boyacá (Colombia). Trees were pruned into a vase shape, globe shape, and natural shape. Physical, chemical, and biological soil analyses were carried out. (3) Results: In the olive tree, V and G pruning significantly increase the P content in the soil compared to NS, and these pruning forms reduce the OOC significantly in the rhizosphere soil by 87.5% and 78.3%, respectively. In all conditions, the roots established an association with Arbuscular Mycorhizal Fungi and stimulated the presence of other microorganisms, despite the trees being more vegetative than productive in this latitude. (4) Conclusions: The results of the study indicate that, in Colombian conditions, the pruning does not affect the rhizospheric soil conditions.
Views of Consumers, Farmers and Stakeholders on Alternative Dairy Cattle Housing Systems
Authors: Erjavec Karmen , Klopčič Marija
Published: May 2022

Alternative housing systems for dairy cattle have recently emerged, such as compost-bedded packs and artificial floor ones. To determine their acceptability among categories of people with a connection to animal husbandry, this study aimed to identify and compare the acceptability of (alternative) housing systems and attitudes to aspects of housing systems for dairy cattle among Slovenian consumers, farmers, and stakeholders. Farmers (N = 306), consumers (N = 508), and stakeholders (N = 40) were interviewed about their views on the main aspects (animals, farmers, products, environment) of housing systems for dairy cattle, the acceptance of four housing systems, and important housing features. The results show that consumers, stakeholders, and conventional farmers preferred housing systems with an artificial floor, while organic farmers preferred a housing system with a compost-bedded pack. Consumers and organic farmers expressed the greatest acceptance of almost every aspect of the housing system, except for a sufficient income for farmers and a low workload. Conventional farmers and stakeholders hold similar views, except for the expectation that the animals have enough space to move around, the image of the landscape, and the animals’ health and wellbeing, where stakeholders showed more acceptance than conventional farmers. The results imply that systematically planned information aimed at different target categories is needed to increase the acceptance of (alternative) housing systems for cattle.
Turnover Rates and Diet–Tissue Discrimination Factors of Nitrogen and Carbon Stable Isotopes in Seahorse Hippocampus reidi Juveniles Following a Laboratory Diet Shift
Authors: Hernández-Urcera Jorge , Dias Carneiro Mario Davi , Planas Miquel
Published: May 2022

The initial development of seahorse juveniles is characterized by low digestion capabilities. Stable isotope analysis is an effective tool in studies of trophic food webs and animal feeding patterns. The present study provides new insights for the understanding of growth and food assimilation in early developing seahorses following a laboratory diet switch. The study was performed in the early life stages of the seahorse Hippocampus reidi by assessing the influence of diet shift on changes and turnovers in carbon (δ13C) and nitrogen (δ15N) stable isotope in juveniles. Newborn seahorses were fed for 60 days following two feeding schedules (A6 and A11) based initially on copepods Acartia tonsa and subsequently on Artemia nauplii (since days 6 and 11, respectively). After the prey shift, we determined δ13C and δ15N turnover rates as functions of change in either body mass (fitting model G) and days of development (fitting model D), contributions of metabolism and growth to those turnover rates, and diet–tissue discrimination factors. Survival, final dry weight, and final standard length for diet A11 were higher compared to diet A6. The shift from copepods to Artemia led to fast initial enrichments in δ13C and δ15N. Afterwards, the enrichment was gradually reduced until the isotopic equilibrium with the diet was reached. In most cases, both fitting models performed similarly. The isotopic analysis revealed that 100% of tissue turnover was attributed to growth in diet A11, whereas 19–25% was linked to metabolism in diet A6. Diet–tissue discrimination factors were estimated for the first time in seahorse juveniles, resulting in higher estimates for diet A11 (2.9 ± 0.7‰ for δ13C; 2.5 ± 0.2‰ for δ15N) than in diet A6 (1.8 ± 0.1‰ for δ13C; 1.9 ± 0.1‰ for δ15N). This study highlights the relevance of feeding on copepods and their effect on isotopic patterns and discrimination factors in seahorse juveniles after a dietary shift. Regarding the application of the results achieved in relation to the feeding schedules in the rearing of H. reidi, a long period of feeding on copepods during the first days of development is highly recommended.
Body Weight Prediction from Linear Measurements of Icelandic Foals: A Machine Learning Approach
Authors: Satoła Alicja , Łuszczyński Jarosław , Petrych Weronika , Satoła Krzysztof
Published: May 2022

Knowledge of the body weight of horses permits breeders to provide appropriate feeding and care regimen and allows veterinarians to monitor the animals’ health. It is not always possible to perform an accurate measurement of the body weight of horses using horse weighbridges, and therefore, new body weight formulas based on biometric measurements are required. The objective of this study is to develop and validate models for estimating body weight in Icelandic foals using machine learning methods. The study was conducted using 312 data records of body measurements on 24 Icelandic foals (12 colts and 12 fillies) from birth to 404 days of age. The best performing model was the polynomial model that included features such as heart girth, body circumference and cannon bone circumference. The mean percentage error for this model was 4.1% based on cross-validation and 3.8% for a holdout dataset. The body weight of Icelandic foals can also be estimated using a less complex model taking a single trait defined as the square of heart girth multiplied by body circumference. The mean percentage error for this model was up to 5% both for the training and the holdout datasets. The study results suggest that machine learning methods can be considered a useful tool for designing models for the estimation of body weight in horses.
A Review: Haemonchus contortus Infection in Pasture-Based Sheep Production Systems, with a Focus on the Pathogenesis of Anaemia and Changes in Haematological Parameters
Authors: Flay Kate J. , Hill Fraser I. , Muguiro Daniela Hernandez
Published: May 2022

Haemonchosis is an important cause of anaemia in sheep worldwide, particularly those that are kept in pasture-based systems in warm, high rainfall environments. Potential outcomes vary based on the severity of infection and the sheep’s immune response, however, in some sheep infection can lead to death. The consequences of Haemonchus contortus infection mean that it has been well-studied in a range of different farming systems. However, to our knowledge, there has not been a recent review focused on the pathophysiology of anaemia caused by haemonchosis. Thus, this review provides an in-depth discussion of the literature related to the pathophysiology of haemonchosis and associated clinical signs for hyperacute, acute, and chronic haemonchosis. Additionally, haematological and biochemical findings are presented, and various diagnostic methods are assessed.
Establishment of a Wisent (Bison bonasus) Germplasm Bank
Authors: Duszewska Anna Maria , Baraniewicz-Kołek Magdalena , Wojdan Jarosław , Barłowska Katarzyna , Bielecki Wojciech , Gręda Paweł , Niżański Wojciech , Olech Wanda
Published: May 2022

The wisent, or European bison (Bison bonasus), belongs to the same family (Bovidae) as the American bison and domestic cattle. The wisent is the largest mammal in Europe, and is called the “Forest Emperor”. The wisent is listed as “Vulnerable” on the IUCN Red List, and is protected by international law. Achievements in reproductive biotechnology have opened new possibilities for the cryoconservation of the wisent germplasm. Therefore, this research aimed to improve a strategy for the protection and preservation of the European bison through the creation of a wisent germplasm bank, based on the following procedures: isolation and in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, in vitro embryo culture (IVC), and embryo cryopreservation. Wisent ovaries were isolated from females outside the reproductive season, and eliminated from breeding for reasons other than infertility. Cumulus–oocyte complexes (COCs) were isolated from follicles greater than 2 mm in diameter and matured for 24 h and 30 h. After IVM, COCs were fertilized in vitro with wisent sperm. The obtained wisent zygotes, based on oocytes matured for 24 h and 30 h, were cultured for 216 h. Embryos at the morula and early blastocyst stages were vitrified and then warmed and transferred to interspecies recipients (Bos taurus). USG and biochemical tests were used to monitor pregnancies. This study obtained embryos in the morula and early blastocyst stages only after oocytes were fertilized and matured for 30 h. On average, per oocyte donor, 12.33 ± 0.5 COCs were isolated, and only 9.33 ± 0.61 COCs were qualified for in vitro maturation (75.68%), while 9.16 ± 0.48 COCs were matured (84.32%). On average, per donor, 5.5 ± 0.34 embryos were cleaved (59.96%) after 48 h post-fertilization (hpf), and 3.33 ± 0.21 achieved the eight-cell stage (36.52%) after 96 hpf, while 1 ± 0.21 morula and early blastocyst stages (10.71%) were achieved after 216 hpf. A total of six embryos (one morula and five early blastocysts) were obtained and vitrified; after warming, five of them were interspecies transferred to cattle (Bos taurus). On day 41 after fertilization, 3 out of 5 pregnancies were detected based on USG, P4, and PAG tests. However, no pregnancy was observed on day 86 after fertilization, indicating embryo resorption. This study shows that obtaining wisent embryos in vitro, and subsequent cryopreservation to create a wisent embryo bank, can be applied and implemented for the wisent protection program.
Transcriptome Analysis of Breast Muscle Reveals Pathways Related to Protein Deposition in High Feed Efficiency of Native Turkeys
Authors: Pezeshkian Zahra , Mirhoseini Seyed Ziaeddin , Ghovvati Shahrokh , Ebrahimie Esmaeil
Published: May 2022

Feed efficiency is important due to the high cost of food, which accounts for about 70% of the total cost of a turkey breeding system. Native poultry are an important genetic resource in poultry breeding programs. This study aimed to conduct a global transcriptome analysis of native male turkeys which have been phenotyped for high and low feed efficiency. Feed efficiency traits were recorded during the experimental period. After slaughter, the three most efficient and three least efficient male turkeys were selected for RNA-Seq analysis. A total of 365 genes with different expressions in muscle tissue were identified between turkeys with a high feed efficiency compared to turkeys with a low feed efficiency. In the pathway analysis of up-regulated genes, major pathways included the “metabolism of glycine, serine, and threonine”; the “adipocytokine signaling pathway” and the “biosynthesis of amino acids”. In the pathway analysis of down-regulated genes, the major pathways included “dorso-ventral axis formation” and “actin cytoskeleton regulation”. In addition, gene set enrichment analyses were performed, which showed that high feed efficiency birds exhibit an increased expression of genes related to the biosynthesis of amino acids and low feed efficiency birds an increased expression of genes related to the immune response. Furthermore, functional analysis and protein network interaction analysis revealed that genes including GATM, PSAT1, PSPH, PHGDH, VCAM1, CD44, KRAS, SRC, CAV3, NEDD9, and PTPRQ were key genes for feed efficiency. These key genes may be good potential candidates for biomarkers of feed efficiency in genetic selection in turkeys.