bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation of Environmentally Friendly BiVO4@SiO2 Encapsulated Yellow Pigment with Remarkable Thermal and Chemical Stability
Authors: Chen Renhua , Zhang Xiaozhen , Tao Rui , Jiang Yuhua , Liu Huafeng , Cheng Lanlan
Published: December 2023

The preparation of environmentally friendly inorganic encapsulated pigments with a bright color and sufficient stability provides an effective strategy for expanding their applications in plastic, paint, glass, and ceramic decoration. The challenges facing the use of such pigments include the formation of a dense protective coating with the required endurance, the relatively weak color of the encapsulated pigments, and the preferable inclusion particle size. Environmentally friendly BiVO4 is regarded as a very promising pigment for multiple coloring applications due to its brilliant yellow color with high saturation. However, its poor thermal and chemical stability greatly limit the application of BiVO4. Herein, we report a sol–gel method to synthesize inorganic BiVO4@SiO2 yellow pigment with a core–shell structure. By controlling the synthesis conditions, including the particle size and dispersion of BiVO4 and the calcination temperature, a BiVO4@SiO2 encapsulated pigment with excellent chromatic properties was achieved. The obtained environmentally friendly BiVO4@SiO2 pigment with encapsulation modification has a comparable color-rendering performance to BiVO4, and it has a high thermal stability at 700 °C, excellent acid resistance, and good compatibility in plastics. The present research is expected to expand the application of yellow BiVO4 pigment in harsh environments.
Short-Term Changes in Arterial Stiffness Measured by 2D Speckle Tracking in Patients Undergoing Transcatheter Aortic Valve Implantation
Authors: Arnold Leonie , Haas Nikolaus Alexander , Jakob André , Fischer Julius , Massberg Steffen , Deseive Simon , Oberhoffer Felix Sebastian
Published: December 2023

Arterial stiffness has received increasing interest as a cardiovascular marker in patients with aortic valve stenosis (AS). So far, studies on the impact of aortic valve replacement (AVR) on arterial stiffness have been equivocal. Two-dimensional speckle tracking (2DST) is a novel, non-invasive method to measure the motion of the vessel wall. In this prospective observational study, we aimed to assess the change in arterial stiffness of the common carotid artery (CCA) measured by 2DST in patients undergoing transcatheter aortic valve implantation (TAVI). A total of 47 patients were included in the study (age 80.04 ± 6.065 years). Peak circumferential strain (CS) was significantly improved after TAVI (4.50 ± 2.292 vs. 5.12 ± 2.958, p = 0.012), as was the peak strain rate (CSR) (0.85 ± 0.567 vs. 1.35 ± 0.710, p = 0.002). Body mass index (BMI), mean arterial pressure (MAP) and hemodynamic parameters were associated with this change. 2DST results did not correlate with aortic pulse wave velocity (aPWV) or augmentation index normalized to heart rate (AIx@75), suggesting a distinct difference between arterial stiffness of the CCA and other stiffness parameters. 2DST seems to be a promising new tool to assess arterial stiffness in TAVI patients.
The Role of IL-23 Inhibitors in Crohn’s Disease
Authors: Fanizza Jacopo , D’Amico Ferdinando , Lusetti Francesca , Fasulo Ernesto , Allocca Mariangela , Furfaro Federica , Zilli Alessandra , Parigi Tommaso Lorenzo , Radice Simona , Peyrin-Biroulet Laurent , Danese Silvio , Fiorino Gionata
Published: December 2023

Promoting a Th17 pathogenic response, the interleukin (IL)-23 pathway is crucial in the pathophysiology of inflammatory bowel disease (IBD). With a favorable safety profile, ustekinumab, a monoclonal antibody targeting the shared p40 component of IL-12/23, is currently approved for the treatment of IBD in patients with disease refractory to corticosteroids and biologic drugs. Risankizumab, mirikizumab, and guselkumab are specific IL-23p19 antagonists tested for the treatment of Crohn’s disease (CD). However, only risankizumab currently has been approved for its treatment. Trials with guselkumab and mirikizumab are currently ongoing, with promising preliminary efficacy and safety results. In this review, we provide a summary of the current knowledge about selective IL-23 inhibitors, focusing on their positioning in the therapeutic algorithm of patients with moderate to severe CD.
Blood-Induced Arthropathy: A Major Disabling Complication of Haemophilia
Authors: Leuci Alexandre , Dargaud Yesim
Published: December 2023

Haemophilic arthropathy (HA) is one of the most serious complications of haemophilia. It starts with joint bleeding, leading to synovitis which, in turn, can cause damage to the cartilage and subchondral bone, eventually inducing degenerative joint disease. Despite significant improvements in haemophilia treatment over the past two decades and recent guidelines from ISTH and WFH recommending FVIII trough levels of at least 3 IU/dL during prophylaxis, patients with haemophilia still develop joint disease. The pathophysiology of HA is complex, involving both inflammatory and degenerative components. Early diagnosis is key for proper management. Imaging can detect joint subclinical changes and influence prophylaxis. Magnetic resonance imagining (MRI) and ultrasound are the most frequently used methods in comprehensive haemophilia care centres. Biomarkers of joint health have been proposed to determine osteochondral joint deterioration, but none of these biomarkers has been validated or used in clinical practice. Early prophylaxis is key in all severe haemophilia patients to prevent arthropathy. Treatment is essentially based on prophylaxis intensification and chronic joint pain management. However, there remain significant gaps in the knowledge of the mechanisms responsible for HA and prognosis-influencing factors. Better understanding in this area could produce more effective interventions likely to ultimately prevent or attenuate the development of HA.
Practical Utility of a Clinical Pathway for Older Patients with Aspiration Pneumonia: A Single-Center Retrospective Observational Study
Authors: Araki Taisuke , Yamazaki Yoshitaka , Kimoto Masanobu , Goto Norihiko , Ikuyama Yuichi , Takahashi Yuko , Kosaka Makoto
Published: December 2023

Introduction: Clinical pathways (CPWs) are patient management tools based on a standardized treatment plan aimed at improving quality of care. This study aimed to investigate whether CPW-guided treatment has a favorable impact on the outcomes of hospitalized older patients with aspiration pneumonia. Method: This retrospective study included patients with aspiration pneumonia, aged ≥ 65 years, and hospitalized at a community hospital in Japan. CPW implementation was arbitrarily determined by the attending physician upon admission. Outcomes were compared according to with or without the CPW (CPW-group and non-CPW groups). Propensity score (PS)-based analyses were used to control for confounding factors. Logistic regression analyses were conducted to evaluate the impact of CPW on the clinical course and outcomes. Results: Of 596 included patients, 167 (28%) received the CPW-guided treatment. The mortality rate was 16.4%. In multivariable model, CPW implementation did not increase the risk for total and 30-day mortality, and resulted in shorter antibiotic therapy duration (≤9 days) (PS matching (PSM): odds ratio (OR) 0.50, p = 0.001; inverse provability of treatment weighting (IPTW): OR 0.48, p < 0.001) and length of hospital stay (≤21 days) (PSM: OR 0.67, p = 0.05; IPTW: OR 0.66, p = 0.03). Conclusions: This study support CPW utility in this population.
The Role of High-Flow Nasal Cannula Oxygen Therapy in Exercise Testing and Pulmonary Rehabilitation: A Review of the Current Literature
Authors: Candia Claudio , Lombardi Carmen , Merola Claudia , Ambrosino Pasquale , D’Anna Silvestro Ennio , Vicario Aldo , De Marco Stefania , Molino Antonio , Maniscalco Mauro
Published: December 2023

High-flow nasal cannula (HFNC) has recently emerged as a crucial therapeutic strategy for hypoxemic patients both in acute and chronic settings. Indeed, HFNC therapy is able to deliver higher fractions of inspired oxygen (FiO2) with a heated and humidified gas flow ranging from 20 up to 60 L per minute, in a more comfortable way for the patient in comparison with Conventional Oxygen Therapy (COT). In fact, the flow keeps the epithelium of the airways adequately moisturized, thus positively affecting the mucus clearance. Finally, the flow is able to wash out the carbon dioxide in the dead space of the airways; this is also enhanced by a modest positive end-expiratory pressure (PEEP) effect. Recent evidence has shown applications of HFNC in exercise training and chronic settings with promising results. In this narrative review, we explored how HFNC might contribute to enhancing outcomes of exercise training and pulmonary rehabilitation among patients dealing with chronic obstructive pulmonary disease, interstitial lung diseases, and lung cancer.
New Horizons of Cementless Total Knee Arthroplasty
Authors: Polizzotti Giuseppe , Lamberti Alfredo , Mancino Fabio , Baldini Andrea
Published: December 2023

Background: Considering the increasing number of young and active patients needing TKA, orthopedic surgeons are looking for a long-lasting and physiological bond for the prosthetic implant. Multiple advantages have been associated with cementless fixation including higher preservation of the native bone stock, avoidance of cement debris with subsequent potential third-body wear, and the achievement of a natural bond and osseointegration between the implant and the bone that will provide a durable and stable fixation. Discussion: Innovations in technology and design have helped modern cementless TKA implants to improve dramatically. Better coefficient of friction and reduced Young’s modulus mismatch between the implant and host bone have been related to the use of porous metal surfaces. Moreover, biologically active coatings have been used on modern implants such as periapatite and hydroxyapatite. These factors have increased the potential for ingrowth by reducing micromotion and increasing osteoconductive properties. New materials with better biocompatibility, porosity, and roughness have been introduced to increase implant stability. Conclusions: Innovations in technology and design have helped modern cementless TKA implants improve primary stability in both the femur and tibia. This means that short-term follow-up are comparable to cemented. These positive prognostic factors may lead to a future in which cementless fixation may be considered the gold-standard technique in young and active patients.
Comparative Analysis of the Visual, Refractive and Aberrometric Outcome with the Use of 2 Intraocular Refractive Segment Multifocal Lenses
Authors: Markuszewski Bartłomiej , Wylęgała Adam , Szentmáry Nóra , Langenbucher Achim , Markuszewska Anna , Wylęgała Edward
Published: December 2023

To demonstrate the results of ray tracing higher- and lower-order aberrations in pseudophakic eyes with rotationally asymmetrical segment multifocal lenses, total high- and low-order aberrations, measured by root mean square value (RMS), refraction, uncorrected distance and uncorrected near visual acuity (UCDVA and UCNVA), and tear break-up time, were measured at scotopic size in 42 eyes of patients implanted with bifocal refractive Mplus15/Mplus30 IOL with +1.5 dpt near addition (42 eyes of patients implanted with Mplus15)/+3.0 dpt near addition (91 eyes of patients implanted with Mplus30), and 107 eyes of control group. No significant differences were noticed between the examined groups concerning UCDVA, UCNVA, and tear break-up time (p < 0.001). Coma and total high-order aberrations were significantly higher for the Mplus30 lens in comparison to the Mplus15 lens and the control group (Coma, Trefoil p < 0.001, Secondary Astigmatism p = 0.002). The spherical aberrations were significantly higher in the lower-addition lens (p = 0.016) in comparison to the control group and to the higher-addition lens group (p < 0.001). Both intraocular lens models were successful at reaching refractive aim, good distance, and near function with the lower higher-order aberrations for the low-addition lens.
Clinical Manifestations and Management of Fibrotic Pulmonary Sarcoidosis
Authors: Kim Jin Sun , Gupta Rohit
Published: December 2023

Fibrotic pulmonary sarcoidosis represents a distinct and relatively uncommon manifestation within the spectrum of sarcoidosis and has substantial morbidity and mortality. Due to the scarcity of research focused on this specific disease subtype, our current understanding of pathogenesis and optimal management remains constrained. This knowledge gap underscores the need for further investigation into areas such as targeted therapies, lung transplantation, and quality of life of patients with fibrotic pulmonary sarcoidosis. The primary aim of this review is to discuss recent developments within the realm of fibrotic pulmonary sarcoidosis to foster a more comprehensive understanding of the underlying mechanisms, prognosis, and potential treatment modalities.
Pediatric Psoriasis with or without Arthritis: Does It Make a Difference?
Authors: Ollech Ayelet , Rotenberg Mor , Tirosh Irit , Bar-Ilan Efrat , Solomon Michal , Greenberger Shoshana , Pavlotsky Felix
Published: December 2023

Background: Psoriasis and psoriatic arthritis can present simultaneously or separately in children and may pose a diagnostic challenge. Objective: To compare the dermatological manifestations in pediatric psoriatic patients with and without arthritis. Methods: A retrospective case-control study of psoriatic patients ≤ 18 years old at Sheba Medical Center was conducted between 2011 and 2021. Patients with psoriatic arthritis versus psoriasis-only were compared according to body surface area (BSA) involvement, cutaneous distribution, severity of skin disease, response to treatment and related side effects. Results: The study cohort included 29 psoriatic arthritis and 64 psoriasis-only patients matched by age and sex. The psoriasis-only group had a significantly higher mean BSA (19.7%, SD ± 18.7) than the psoriatic arthritis group (6.1%, SD ± 11.4), (p = 0.029). The skin distribution differed with the psoriasis group showing more involvement of the extremities, scalp, trunk, and genitals. Both groups primarily experienced partial responses to methotrexate, whereas the psoriasis group mainly saw complete responses to biologics. Adverse events were rare, with a higher incidence in the psoriasis group. Conclusion: This retrospective study highlights the differences in cutaneous disease characteristics, severity, and treatment response in pediatric patients with psoriasis and psoriatic arthritis, providing valuable insights for diagnosis and disease course in the pediatric population.
Therapeutic Management of Children with Vesicoureteral Reflux
Authors: Chirico Valeria , Tripodi Filippo , Lacquaniti Antonio , Monardo Paolo , Conti Giovanni , Ascenti Giorgio , Chimenz Roberto
Published: December 2023

Contrasting data refer to therapies for vesicoureteral reflux (VUR), such as surgical treatments and continuous antibiotic prophylaxis (CAP). This study evaluated the effectiveness of these approaches in children with VUR, analyzing the recurrence of febrile urinary tract infections (UTIs) and the resolution of VUR after the treatment. A total of 350 pediatric patients underwent contrast-enhanced voiding urosonography (ceVUS) to diagnose a VUR, whereas renal scintigraphy evaluated potential scars. After 12 months from the treatment, the VUR, the relapse of febrile UTIs, and reflux-related nephropathy were analyzed. Twenty-seven children had recurrent febrile UTIs after surgical therapy, with a greater rate of relapses observed in III and V VUR grades. Thirteen patients who underwent surgery had scars, independently of VUR grades and gender, with evidence of chronic renal failure at the end of the follow-up period. A total of 140 subjects were treated with CAP, and 30% of them continued to suffer from febrile UTIs. Ninety-five patients with VUR underwent ceVUS after 12 months, with persistent reflux in fifty-two patients. All of them had severe VUR, correlating with the age at diagnosis and gender. CAP therapy prevented scarring better than surgery, especially in children with III and V grades of VUR. A late onset of VUR or VUR involving neonatal patients is rarely a reversible process. This study identified predictors of success or failure of surgical or CAP therapies, evaluating the relapse of UTIs or persistent reflux after the treatment and giving prognostic information in children with VUR.
Pathogenic Variant Frequencies in Hereditary Haemorrhagic Telangiectasia Support Clinical Evidence of Protection from Myocardial Infarction
Authors: Jain Kinshuk , McCarley Sarah C. , Mukhtar Ghazel , Ferlin Anna , Fleming Andrew , Morris-Rosendahl Deborah J. , Shovlin Claire L.
Published: December 2023

Hereditary haemorrhagic telangiectasia (HHT) is a vascular dysplasia inherited as an autosomal dominant trait, due to a single heterozygous loss-of-function variant, usually in ACVRL1 (encoding activin receptor-like kinase 1 [ALK1]), ENG (encoding endoglin [CD105]), or SMAD4. In a consecutive single-centre series of 37 positive clinical genetic tests performed in 2021–2023, a skewed distribution pattern was noted, with 30 of 32 variants reported only once, but ACVRL1 c.1231C>T (p.Arg411Trp) identified as the disease-causal gene in five different HHT families. In the same centre’s non-overlapping 1992–2020 series where 110/134 (82.1%) HHT-causal variants were reported only once, ACVRL1 c.1231C>T (p.Arg411Trp) was identified in nine further families. In a 14-country, four-continent HHT Mutation Database where 181/250 (72.4%) HHT-causal variants were reported only once, ACVRL1 c.1231C>T (p.Arg411Trp) was reported by 12 different laboratories, the adjacent ACVRL1 c.1232G>A (p.Arg411Gln) by 14, and ACVRL1 c.1120C>T (p.Arg374Trp) by 18. Unlike the majority of HHT-causal ACVRL1 variants, these encode ALK1 protein that reaches the endothelial cell surface but fails to signal. Six variants of this type were present in the three series and were reported 6.8–25.5 (mean 8.9) times more frequently than the other ACVRL1 missense variants (all p-values < 0.0039). Noting lower rates of myocardial infarction reported in HHT, we explore potential mechanisms, including a selective paradigm relevant to ALK1′s role in the initiating event of atherosclerosis, where a plausible dominant negative effect of these specific variants can be proposed. In conclusion, there is an ~9-fold excess of kinase-inactive, cell surface-expressed ACVRL1/ALK1 pathogenic missense variants in HHT. The findings support further examination of differential clinical and cellular phenotypes by HHT causal gene molecular subtypes.
Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses
Authors: Alhotan Abdulaziz , Raszewski Zbigniew , Chojnacka Katarzyna , Mikulewicz Marcin , Kulbacka Julita , Alaqeely Razan , Mirdad Amani , Haider Julfikar
Published: December 2023

The colonisation of the surface of removable acrylic dentures by various types of microorganisms can lead to the development of various diseases. Therefore, the creation of a bioactive material is highly desirable. This study aimed to develop a denture base material designed to release bioactive ions into the oral environment during use. Four types of bioactive glasses (BAG)—S53P4, Biomin F, 45S5, and Biomin C—were incorporated into the PMMA acrylic resin, with each type constituting 20 wt.% (10 wt.% non-silanised and 10% silanised) of the mixture, while PMMA acrylic resin served as the control group. The specimens were subsequently immersed in distilled water, and pH measurements of the aqueous solutions were taken every seven days for a total of 38 days. Additionally, surface roughness and translucency measurements were recorded both after preparation and following seven days of immersion in distilled water. The cytotoxicity of these materials on human fibroblast cells was evaluated after 24 and 48 h using Direct Contact and MTT assays. Ultimately, the elemental composition of the specimens was determined through energy-dispersive X-ray (EDX) spectroscopy. In general, the pH levels of water solutions containing BAG-containing acrylics gradually increased over the storage period, reaching peak values after 10 days. Notably, S53P4 glass exhibited the most significant increase, with pH levels rising from 5.5 to 7.54. Surface roughness exhibited minimal changes upon immersion in distilled water, while a slight decrease in material translucency was observed, except for Biomin C. However, significant differences in surface roughness and translucency were observed among some of the BAG-embedded specimens under both dry and wet conditions. The composition of elements declared by the glass manufacturer was confirmed by EDX analysis. Importantly, cytotoxicity analysis revealed that specimens containing BAGs, when released into the environment, did not adversely affect the growth of human gingival fibroblast cells after 48 h of exposure. This suggests that PMMA acrylics fabricated with BAGs have the potential to release ions into the environment and can be considered biocompatible materials. Further clinical trials are warranted to explore the practical applications of these materials as denture base materials.
Numerical Framework for the Coupled Analysis of Floating Offshore Multi-Wind Turbines
Authors: Berdugo-Parada I. , Servan-Camas B. , Garcia-Espinosa J.
Published: December 2023

Floating offshore multi-wind turbines (FOMWTs) are an interesting alternative to the up-scaling of wind turbines. Since this is a novel concept, there are few numerical tools for its coupled dynamic assessment at the present time. In this work, a numerical framework is implemented for the simulation of multi-rotor systems under environmental excitations. It is capable of analyzing a platform using leaning towers that handle wind turbines with their own features and control systems. This tool is obtained by coupling the seakeeping hydrodynamics solver SeaFEM with the single wind turbine simulation tool OpenFAST. The coupling of SeaFEM provides a higher fidelity hydrodynamic solution, allowing the simulation of any structural design using the finite element method (FEM). Additionally, a methodology is proposed for the extension of the single wind solver, allowing for the analysis of multi-rotor configurations. To do so, the solutions of the wind turbines are computed independently using several OpenFAST instances, performing its dynamic interaction through the floater. This method is applied to the single turbine Hywind concept and the twin-turbine W2Power floating platform, supporting NREL 5-MW wind turbines. The rigid-body response amplitude operators (RAOs) are computed and compared with other numerical tools. The results showed consistency in the developed framework. An agreement was also obtained in simulations with aerodynamic loads. This resulting tool is a complete time-domain aero–hydro–servo–elastic solver that is able to compute the combined response and power generation performance of multi-rotor systems.
Sensitivity Analysis of Underwater Structural-Acoustic Problems Based on Coupled Finite Element Method/Fast Multipole Boundary Element Method with Non-Uniform Rational B-Splines
Authors: Cao Yonghui , Zhou Zhongbin , Xu Yanming , Qu Yilin
Published: January 2024

For the direct differentiation technique-based modeling of acoustic fluid–structure interaction and structural-acoustic sensitivity analysis, a coupling algorithm based on the finite element method (FEM) and the fast multipole boundary element method (FMBEM) is suggested. By bypassing the challenging volume parameterization process in isogeometric finite element techniques and the laborious meshing process in traditional FEM/BEM, non-uniform rational B-splines (NURBS) isogeometric analysis (IGA) is utilized to immediately perform numerical analysis on CAD models. The matrix-vector products in the boundary element analysis are accelerated using the fast multipole method (FMM). To hasten the solution of the linear system of equations, the iterative solver GMRES is used. The numerical prediction of the effects of arbitrarily shaped vibrating structures on the sound field is made feasible by the FEM/FMBEM technique. A number of numerical examples are provided to show the applicability and effectiveness of the suggested approach.
Effects of Neutron Flux Distribution and Control Rod Shadowing on Control Rod Calibrations in the Oregon State TRIGA® Reactor
Authors: Spoerer Tracey , Schickler Robert , Reese Steven
Published: January 2024

Control rod calibration experiment results for the Oregon State TRIGA® Reactor (OSTR) immediately following LEU conversion in 2008, and MCNP® 5 predicted rod worths from the 2008 LEU Conversion Safety Analysis Report (CSAR) are discussed. The reactivity worth of the four OSTR control rods is measured using the rod-pull method. Reactor power and period measurements in this method rely on the fission chamber power detector on the north side of the reflector. It is proposed that the location of the fission chamber and the neutron flux distribution in the core may result in an inaccurate reactor period measurement due to the asymmetry of the neutron flux distribution in the OSTR core. The asymmetry of the flux is believed to be more pronounced during super-criticality, resulting in errors in the time-of-power-rise measurements. As a result, control rod calibration experiments may under-predict or over-predict the reactivity worth of certain control rods. A time-independent Monte–Carlo method for the quantification of these effects is presented. Thermal flux maps at the core axial mid-plane are obtained from the model to inform discrepancies between predicted and observed results.
A Novel Mitochondrial Targeted Compound Phosundoxin Showing Potent Antifungal Activity against Common Clinical Pathogenic Fungi
Authors: Zhang Shu , Geng Yuanyuan , Wei Bin , Lu Yangzhen , He Lihua , Zhao Fei , Zhang Jianzhong , Qin Zhaohai , Gong Jie
Published: December 2023

The current increase in resistance to antifungal drugs indicates that there is an urgent need to explore novel antifungal drugs with different mechanisms of action. Phosundoxin is a biphenyl aliphatic amide using a TPP-targeting strategy which targets mitochondria. To provide insights into the antifungal activities of phosundoxin, the antifungal susceptibility testing of phosundoxin was conducted on 158 pathogenic fungi and compared to that of traditional azole drugs. Phosundoxin displayed a broad-spectrum antifungal activity on all the tested yeast-like and filamentous fungi ranging from 2 to 16 mg/L. In particular, azole-resistant clinical isolates of Candida albicans were susceptible to phosundoxin with the same MICs as azole-susceptible C. albicans. Transcriptome analysis on azole-resistant C. albicans identified 554 DEGs after treatment with phosundoxin. By integrating GO and KEGG pathway enrichment analysis, the antifungal activity of phosundoxin was related to impairment of mitochondrial respiratory chain function. Acute oral and percutaneous toxicity of phosundoxin to rats showed that the compound phosundoxin were mild toxicity and LD50 was above 5000 mg/kg body weight in rats. This study demonstrated the potential of phosundoxin as an antifungal agent for the treatment of common fungal infection and contributed to providing insights into the mechanisms of action of phosundoxin against C. albicans.
Comparative Genomic Analysis of Colletotrichum lini Strains with Different Virulence on Flax
Authors: Dvorianinova Ekaterina M. , Sigova Elizaveta A. , Mollaev Timur D. , Rozhmina Tatiana A. , Kudryavtseva Ludmila P. , Novakovskiy Roman O. , Turba Anastasia A. , Zhernova Daiana A. , Borkhert Elena V. , Pushkova Elena N. , Melnikova Nataliya V. , Dmitriev Alexey A.
Published: December 2023

Colletotrichum lini is a flax fungal pathogen. The genus comprises differently virulent strains, leading to significant yield losses. However, there were no attempts to investigate the molecular mechanisms of C. lini pathogenicity from high-quality genome assemblies until this study. In this work, we sequenced the genomes of three C. lini strains of high (#390-1), medium (#757), and low (#771) virulence. We obtained more than 100× genome coverage with Oxford Nanopore Technologies reads (N50 = 12.1, 6.1, 5.0 kb) and more than 50× genome coverage with Illumina data (150 + 150 bp). Several assembly strategies were tested. The final assemblies were obtained using the Canu–Racon ×2–Medaka–Polca scheme. The assembled genomes had a size of 54.0–55.3 Mb, 26–32 contigs, N50 values > 5 Mb, and BUSCO completeness > 96%. A comparative genomic analysis showed high similarity among mitochondrial and nuclear genomes. However, a rearrangement event and the loss of a 0.7 Mb contig were revealed. After genome annotation with Funannotate, secreting proteins were selected using SignalP, and candidate effectors were predicted among them using EffectorP. The analysis of the InterPro annotations of predicted effectors revealed unique protein categories in each strain. The assembled genomes and the conducted comparative analysis extend the knowledge of the genetic diversity of C. lini and form the basis for establishing the molecular mechanisms of its pathogenicity.
Clinical and Molecular Characterization of a Patient with Generalized Arterial Calcification of Infancy Caused by Rare ABCC6 Mutation
Authors: Yao Ruen , Yang Fan , Zhang Qianwen , Yu Tingting , Yu Ying , Chang Guoying , Wang Xiumin
Published: December 2023

Generalized arterial calcification of infancy (GACI) is a rare autosomal-recessive disease characterized by extensive arterial calcification in infancy, with clinical manifestations such as arterial stenoses and heart failure. The ENPP1 inactivation mutation has been identified as a potential defect in most of the cases of GACI, while mutations in ABCC6 are demonstrated in patients who are genotyped as pseudoxanthoma elasticum and only limited cases of GACI are reported. Whole-exome sequencing was applied for the detection of pathogenic variants. Copy-number variants of pathogenic genes were also evaluated through a bioinformatic process and were further validated by real-time quantitative PCR. In this report, we described the clinical information and treatment of a patient with extensive arterial calcification. We have identified the underlying cause as biallelic mutations in ABCC6 (NM_00117: exon30, c.4223_4227dupAGCTC p.(Leu1410Serfs*56)) and a unique exonic deletion that spans from the first to the fourth exons of ABCC6 (chr16:16313388-16330869)). This discovery was made by utilizing a combined genetic testing approach. With the review of previously reported GACI patients with ABCC6 mutation, our work contributed to enriching the mutation spectrum of GACI and providing further information on this rare form of inherited disorder.
Personalized Noninvasive Respiratory Support in the Perioperative Setting: State of the Art and Future Perspectives
Authors: Misseri Giovanni , Frassanito Luciano , Simonte Rachele , Rosà Tommaso , Grieco Domenico Luca , Piersanti Alessandra , De Robertis Edoardo , Gregoretti Cesare
Published: December 2023

Background: Noninvasive respiratory support (NRS), including high-flow nasal oxygen therapy (HFNOT), noninvasive ventilation (NIV) and continuous positive airway pressure (CPAP), are routinely used in the perioperative period. Objectives: This narrative review provides an overview on the perioperative use of NRS. Preoperative, intraoperative, and postoperative respiratory support is discussed, along with potential future areas of research. Results: During induction of anesthesia, in selected patients at high risk of difficult intubation, NIV is associated with improved gas exchange and reduced risk of postoperative respiratory complications. HFNOT demonstrated an improvement in oxygenation. Evidence on the intraoperative use of NRS is limited. Compared with conventional oxygenation, HFNOT is associated with a reduced risk of hypoxemia during procedural sedation, and recent data indicate a possible role for HFNOT for intraoperative apneic oxygenation in specific surgical contexts. After extubation, “preemptive” NIV and HFNOT in unselected cohorts do not affect clinical outcome. Postoperative “curative” NIV in high-risk patients and among those exhibiting signs of respiratory failure can reduce reintubation rate, especially after abdominal surgery. Data on postoperative “curative” HFNOT are limited. Conclusions: There is increasing evidence on the perioperative use of NRS. Use of NRS should be tailored based on the patient’s specific characteristics and type of surgery, aimed at a personalized cost-effective approach.
Personalized Predictive Hemodynamic Management for Gynecologic Oncologic Surgery: Feasibility of Cost–Benefit Derivatives of Digital Medical Devices
Authors: Frassanito Luciano , Di Bidino Rossella , Vassalli Francesco , Michnacs Kristian , Giuri Pietro Paolo , Zanfini Bruno Antonio , Catarci Stefano , Filetici Nicoletta , Sonnino Chiara , Cicchetti Americo , Arcuri Giovanni , Draisci Gaetano
Published: December 2023

Background: Intraoperative hypotension is associated with increased perioperative complications, hospital length of stay (LOS) and healthcare expenditure in gynecologic surgery. We tested the hypothesis that the adoption of a machine learning-based warning algorithm (hypotension prediction index—HPI) might yield an economic advantage, with a reduction in adverse outcomes that outweighs the costs for its implementation as a medical device. Methods: A retrospective-matched cohort cost–benefit Italian study in gynecologic surgery was conducted. Sixty-six female patients treated with standard goal-directed therapy (GDT) were matched in a 2:1 ratio with thirty-three patients treated with HPI based on ASA status, diagnosis, procedure, surgical duration and age. Results: The most relevant contributor to medical costs was operating room occupation (46%), followed by hospital stay (30%) and medical devices (15%). Patients in the HPI group had EURO 300 greater outlay for medical devices without major differences in total costs (GDT 5425 (3505, 8127), HPI 5227 (4201, 7023) p = 0.697). A pre-specified subgroup analysis of 50% of patients undergoing laparotomic surgery showed similar medical device costs and total costs, with a non-significant saving of EUR 1000 in the HPI group (GDT 8005 (5961, 9679), HPI 7023 (5227, 11,438), p = 0.945). The hospital LOS and intensive care unit stay were similar in the cohorts and subgroups. Conclusions: Implementation of HPI is associated with a scenario of cost neutrality, with possible economic advantage in high-risk settings.
Evolution Model, Mechanism, and Performance of Urban Park Green Areas in the Grand Canal of China
Authors: Cai Zihan , Zhao Sidong , Huang Mengshi , Zhang Congguo
Published: December 2023

Urban park green areas are part of territorial space planning, shouldering the mission of providing residents with high-quality ecological products and public space. Using a combination of several measurement models such as the BCG (Boston Consulting Group) matrix, ESDA (Exploratory Spatial Data Analysis), MLR (Machine Learning Regression), GWR (Geographically Weighted Regression), and GeoDetector, this paper presents an empirical study on the changes in Urban Park Green Areas (UPGAs) in the Grand Canal of China. By quantitatively measuring the spatio–temporal evolution patterns of UPGAs, this study reveals the driving mechanisms behind them and proposes policy recommendations for planning and management based on performance evaluation. The spatio–temporal evolution of UPGAs and their performance in China’s Grand Canal are characterized by significant spatial heterogeneity and correlation, with diversified development patterns such as HH (High-scale–High-growth), HL (High-scale–Low-growth), LH (Low-scale–High-growth), and LL (Low-scale–Low-growth) emerging. The evolution performance is dominated by positive oversupply and positive equilibrium, where undersupply coexists with oversupply. Therefore, this paper recommends the implementation of a zoning strategy in the future spatial planning of ecological green areas, urban parks, and green infrastructure. It is also recommended to design differentiated construction strategies and management policies for each zoning area, while promoting inter-city mutual cooperation in the joint preparation and implementation of integrated symbiosis planning. Furthermore, the spatio–temporal evolution of the UPGAs in the Grand Canal of China is influenced by many factors with very complex dynamic mechanisms, and there are significant differences in the nature, intensity, spatial effects, and interaction effects between different factors. Therefore, in the future management of ecological green areas, urban parks, and green infrastructure, it is necessary to interconnect policies to enhance their synergies in population, aging, industry and economy, and ecological civilization to maximize the policy performance.
Multifunctional Use of Agricultural Land with Support for Selected Ecosystem Services in the Territory Protected Water Management Area Žitný Ostrov
Authors: Krnáčová Zdena , Kenderessy Pavol , Baránková Zuzana , Barančoková Mária , Labuda Martin
Published: December 2023

Agroecosystems represent one of the largest managed systems in the world and are also considered to be the largest surface polluters. This is particularly evident in highly vulnerable areas such as the model area assessed in our study, the Protected Water Management Area Žitný Ostrov, which contains the most productive soils in the Slovak Republic. At the same time, the Žitný Ostrov represents the largest groundwater reserves in Central Europe. We based this study on the information system of valued soil ecological units (VSEUs) and their spatial extension to the agricultural soils of Slovakia. We used the synthetic–parametric method for a summary (aggregated) evaluation of the production potential of provisioning soil services, which we have classified into 10 categories (1—very low potential; 10—very high potential). Overall, there were 3000 individual VSEUs identified in the area of interest. By eliminating repetitive VSEUs, we evaluated 72 subtypes and, based on similarity parameters, they were further aggregated into 40 soil subtypes. The result is a spatial representation of the proposed zones within the model area (zones for sustainable economic use of soils with the support for ecologization of agricultural technologies, non-productive land use, cultural and social development of rural areas, and the protection and revitalization of the landscape and its components). The approach is also suitable for use in other areas.
Is Abandoned Cropland Continuously Growing in China? Quantitative Evidence and Enlightenment from Landsat-Derived Annual China Land Cover Dataset
Authors: Wang Haoran , Guo Zecheng , Xie Yaowen , Zhang Xueyuan , Xi Guilin , Huang Hongxin
Published: December 2023

Abandoned cropland is a great waste of land resources, which not only affects the stability of food production, but also wastes limited cropland resources. China has large areas of abandoned cropland, and accurately extracting the large-scale abandoned cropland is a prerequisite for the government to implement reclamation policies and estimate the food production potential of abandoned cropland. China has implemented the Grain for Green Project (GGP) since 1980, but most research has not considered its impact on the extraction of abandoned cropland. Therefore, a method was proposed to identify abandoned cropland excluding the regions of GGP, and to analyze the spatio-temporal characteristics and potential of abandoned cropland in China from 1991 to 2018. GGP potential and food production potential of abandoned cropland were discussed. The results showed that 26.47 million hectares of cropland has been abandoned in the past 28 years (including cropland that has been reclaimed). Under the positive influence of the government’s food security policy, the abandoned cropland has decreased in recent years, but the total area is still high. The abandonment mainly occurred in the Jiangnan Hills, the Hilly Regions of Fujian and Guangdong Province, the North China Mountains, and Eastern Inner Mongolia. The higher the elevation and the steeper the slope, the higher the abandonment rate. In addition, the urban-rural income gap significantly affected the abandonment rate. This study drew the spatial and temporal distribution map of abandoned cropland in China with a spatial resolution of 30 m considering GGP. It provides an important basis for formulating reclamation and GGP policy.
The Professional Conflict Pertaining to Confidentiality—The Obligation of Disclosure for Intermediaries of Financial Transactions
Authors: Tofan Mihaela , Arseni Alina-Adriana
Published: December 2023

The present study aims at providing an overview of the international, European, and national legal frameworks relating to the obligation of intermediaries of financial transactions to report to tax authorities, and the professional secrecy which applies to their professions, as well as the conflict between the two. The authors address these topics from theoretical and jurisprudential perspectives, both at national and European levels, using doctrinaire, documentary, and comparative approaches. The analyses pointed out that the focus is placed on lawyer–intermediaries’ activities and liabilities when their activity is covered by confidentiality and legal privilege. Specific attention was revealed to be necessary when the conditions under which an exemption from the reporting obligation applies, and the particularities of the effects of the regulation in these scenarios. The topic of observing the legal framework and solving the possible conflicts generated by the divergent regulation of the law enforced has been the subject matter of recent European case laws that impact all the legal systems of the European Union’s member states, which has necessitated an examination of the hierarchy of law systems within the European Union member states and to emphasize the practical jurisprudential effects.
Urinary Incontinence and Other Pelvic Floor Dysfunctions as Underestimated Problems in People under Forty Years: What Is Their Relationship with Sport?
Authors: Bonaldi Lorenza , Mascolini Maria Vittoria , Todesco Martina , Zara Anna , Rossato Camilla , Fede Caterina , Fontanella Chiara Giulia , Stecco Carla
Published: December 2023

Urinary incontinence is still an underestimated problem due to its anatomical complexity and social taboo. Most of the time, it is believed to affect predominantly the elderly female population, and the literature still lacks data on its presence in the younger and male populations. Its relationship with other pelvic floor dysfunctions (PFDs) and sport activity remains an open topic. Thus, the present study surveyed 342 subjects of both genders, ranging from 18 to 39 y/o and with different sport activity levels, to understand the prevalence of PFDs (such as haemorrhoids, anal fissures, involuntary urinary/faecal leakage, and urgency). The results also showed a significative prevalence in younger, sporty, and male people. Approximately one third of the population had urinary incontinence mostly during stress activities (sport activity: 17%, cough/sneeze: 13%). The statistical analysis confirmed a higher prevalence in the cases of a light (32%) and intense (41%) sport activity level and a protective role of sport if practiced between 5 and 10 h/week, with bodybuilding/CrossFit and running seeming to be the riskiest sports. The relationship with the other PFDs showed a statistically significant dependence with most of them, confirming that urinary incontinence cannot be considered a separate problem from the other PFDs.
Nutritional Intervention Improves Muscle Mass and Physical Performance in the Elderly in the Community: A Systematic Review and Meta-Analysis
Authors: Ren Yuanyuan , Lu Aming , Wang Bingqing , Wang Cenyi
Published: December 2023

Nutritional supplements have been extensively used as health interventions for the elderly. However, with the spread of COVID-19, no consensus exists on whether nutritional interventions could improve muscle mass and physical activity in community-dwelling older adults. To conduct a systematic review and meta-analysis to explore the effects of different nutritional interventions on muscle mass and physical performance in the elderly, we searched PubMed, Web of Science, Elsevier, and Cochrane databases from their founding dates to December 2023. The meta-analysis was performed using RevMan5.3 software. Only randomized controlled trials (RCTs) were considered, and the overall mean difference (MD) or standardized mean difference (SMD) with 95% confidence interval (CI) was calculated. There were 33 studies comprising 3579 elderly persons meeting the inclusion criteria. Comprehensive analysis suggested that the intervention effect of fat-free mass (FFM), appendix skeletal muscle mass (ASMM), handgrip strength (HGS), gait speed, and short physical performance battery (SPPB) score was higher in the nutritional supplement group than in the control group. The results of subgroup analysis demonstrated that protein supplementation (SMD = 0.82, p < 0.0001) had an optimal effect on ASMM (SMD = 0.89, p < 0.0001) and FFM (MD = 2.09, p < 0.0001) in the elderly. Vitamin D supplementation (SMD = 0.52, p < 0.0001) had a marginal effect on ASMM, and energy supplementation (SMD = 0.39, p = 0.0005) had the lowest effect. Moreover, nutritional interventions had the most significant impact on HGS (MD = 1.06, p < 0.0001) and TUG (MD = 0.14, p < 0.0001) in individuals aged 65–75 years old, with positive effects on FFM (MD = 1.62, p < 0.0001) and HGS (MD = 0.82, p < 0.0001) when compared to healthy elderly individuals, and had greater effect on ASMM (SMD = 0.69, p < 0.0001) than on the elderly with sarcopenia. Nutritional supplements can enhance muscle mass and physical performance in the elderly, while protein is recommended for muscle function. The golden period for implementing nutritional interventions to improve muscle function is before the age of 75 years. However, the impact of nutritional interventions varies with age and population. Given the limited evidence on nutritional interventions, more detailed and high-quality studies are highly warranted in the future.
A Modelling Approach for the Management of Invasive Species at a High-Altitude Artificial Lake
Authors: Moutopoulos Dimitrios K. , Douligeri Alexandra S. , Ziou Athina , Kiriazis Nikolaos , Korakis Athanasios , Petsis Nikolaos , Katselis George N.
Published: December 2023

The ecosystem structure of a Mediterranean high-altitude artificial lake (Aoos Springs, Northwest Greece) was assessed and quantified using the Ecopath with Ecosim model. The artificial lake was created in 1990 for hydroelectric production and the fish fauna has been enriched across years due to deliberate or accidental fish stockings, as well as the introduction of the invasive pumpkinseed (Lepomis gibbosus), which may cause adverse effects on aquatic ecosystems and their biota. The model considered 20 species/taxa groups with biological data gathered during 2021–2022 from field surveys, interviews with recreational anglers and literature. The results revealed that the artificial lake of Aoos is a resilient ecosystem to unexpected events with low intensity of fisheries exploitation. The biomass of the introduced invasive species Lepomis gibbosus is low, due to the low biomass level of its diet, Chironomidae and zoobenthic organisms as well as other fish species.
(1R/S,7aS/R)-1-Benzyl-1-[2,8-bis(trifluoromethyl)quinolin-4-yl]-hexahydro-oxazolo[3,4-a]pyridin-3-one
Authors: Kucharski Dawid J. , Boratyński Przemysław J.
Published: December 2023

An unexpected diastereoselective C-alkylation of a mefloquine derivative in up to 57% yield was the result of an attempted Williamson etherification of Boc-mefloquine. The domino reaction involved oxazolidinone ring closure, deprotonation, and stereoselective carbon–carbon bond formation. The structure was confirmed with 2D NMR experiments.