EFFECT OF B. ANIMALIS AND B. SUBTILIS ІМВ B-7724 METABOLITES ON THE POLARIZATION STATE OF MACROPHAGES IN EXPERIMENTS IN VIVO
Authors:   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Fedosova N.I. , Cheremshenko N.L. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Gogol S.V. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Chumak A.V. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Symchych T.V. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Voyeykova I.M. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Lykhova О.O. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine, Chekhun V.F. ,   R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv, Ukraine
Published: January 2025

Summary. Aim: to investigate of the effect of bacteria of the genus Bifidobacterium and the extracellular metabolite B. subtilis IMV B-7724 on the process of macrophage polarization in mice with a model tumor. Object and methods: the study was conducted on Balb/c mice (n = 52). Ehrlich adenocarcinoma (ACE) was used as an experimental model. Starting from the 2nd day after the transplantation of tumor cells, animals of the experimental groups were treated with lectin of B. subtilis IMV B-7724 (s/c, 1 mg/kg of weight), B. animalis subsp. lactis BB-12 (per os, 7×105 CFU/mouse) or their combination. On the 21st and 28th days of tumor growth, the parameters of the functional activity of macrophages (Mph) were determined by the level of NO production, arginase (Arg) and cytotoxic activity. Statistical processing of the results was performed using generally accepted methods of variational statistics. Results: at the terminal stage of ACE growth, cells with the M2 phenotype prevailed among the Mph of the peritoneal cavity and tumor tissue. The introduction of the lectin of B. subtilis IMV B-7724 (both separately and in combination with B. animalis) promoted the polarization of peritoneal Mph to the M1 phenotype, as evidenced by an increase (p < 0.05) in the NO/Arg ratio by 5.2 times (on day 21) and by 6.9 times (on day 28). The introduction of B. animalis alone led to the polarization of peritoneal Mph to the M2 phenotype, as evidenced by a significant increase (p < 0.05) in Arg activity and a decrease in the NO/Arg index compared with the indicators of both intact mice and animals of other experimental groups. The polarization of Mph obtained from tumor tissue changed in a similar way. Conclusions: the introduction of lectin of the B. subtilis IMV B-7724 alone or in combination with B. animalis subsp. lactis BB-12 contributed to the change in the polarization state of Mphs of animals with a model tumor. Regardless of the anatomical niche of functioning, cells with the phenotype and functional properties of M1 prevailed among Mphs.