bluuebunny/crossref_metadata_2025 huggingface dataset:
Instruments and Tools to Identify Radical Textual Content
Authors: Mothe Josiane , Ullah Md Zia , Okon Guenter , Schweer Thomas , Juršėnas Alfonsas , Mandravickaitė Justina
Published: April 2022

The Internet and social networks are increasingly becoming a media of extremist propaganda. On homepages, in forums or chats, extremists spread their ideologies and world views, which are often contrary to the basic liberal democratic values of the European Union. It is not uncommon that violence is used against those of different faiths, those who think differently, and members of social minorities. This paper presents a set of instruments and tools developed to help investigators to better address hybrid security threats, i.e., threats that combine physical and cyber attacks. These tools have been designed and developed to support security authorities in identifying extremist propaganda on the Internet and classifying it in terms of its degree of danger. This concerns both extremist content on freely accessible Internet pages and content in closed chats. We illustrate the functionalities of the tools through an example related to radicalisation detection; the data used here are just a few tweets, emails propaganda, and darknet posts. This work was supported by the EU granted PREVISION (Prediction and Visual Intelligence for Security Intelligence) project.
Predictive Value of Elevated Neutrophil Gelatinase-Associated Lipocalin (NGAL) Levels for Assessment of Cardio–Renal Interactions among ST-Segment Elevation Myocardial Infarction Patients
Authors: Zahler David , Merdler Ilan , Banai Ariel , Shusterman Eden , Feder Omri , Itach Tamar , Robb Leemor , Banai Shmuel , Shacham Yacov
Published: April 2022

Background: Elevated serum neutrophil gelatinase-associated lipocalin (NGAL) levels reflect both inflammatory reactions and renal tubular injury. Recently, associations with endothelial dysfunction and plaque instability were also proposed. We investigated the prognostic utility of elevated NGAL levels for renal and clinical outcomes among ST-segment elevation myocardial infarction (STEMI) patients treated with primary coronary intervention (PCI). Methods: We performed a prospective, observational, open-label trial. High NGAL was defined as values within the third tertile (>66 percentile). Results: A total of 267 patients were included (mean age 66 ± 14 years, 81% males). Short-term adverse outcomes were consistently increased in the high NGAL group with more acute kidney injury, lower mean left ventricular ejection fraction, higher 30-day mortality, and higher incidence for the composite outcome of major adverse cardiac events (MACE). In a multivariate logistic regression model, high NGAL emerged as a strong and independent predictor for MACE (OR 2.07, 95% CI 1.15–3.73, p = 0.014). Conclusions: Among STEMI patients undergoing primary PCI, elevated NGAL levels are associated with adverse renal and cardiovascular outcomes, independent of traditional inflammatory markers. Further studies are needed to assess the potentially unique role of NGAL in cardio–renal interactions.
High Heterogeneity of Temporal Bone CT Aspects in Osteogenesis Imperfecta Is Not Linked to Hearing Loss
Authors: Ltaief-Boudrigua Aïcha , Lina-Granade Genevieve , Truy Eric , Hermann Ruben , Chevrel Guillaume
Published: April 2022

Objectives: To determine whether temporal bone computed tomography (CT) features are linked to the presence and type of hearing loss in osteogenesis imperfecta (OI) when considering hearing-impaired OI patients and normally hearing (NH) OI ones. A secondary objective was to assess whether other factors influence CT features in a large sample: age, type of mutation, or bone mineral density (BMD). Methods: A total of 41 adults with OI underwent CTs and pure-tone audiometry in 82 ears. Hearing thresholds were normal in 64 out of 82 ears, and most had not been operated on for stapedectomy or stapedotomy. Ossicle density, footplates, oval and round windows, retrofenestral peri- and endolabyrinths, and temporal pneumatization were analyzed twice by an experienced radiologist. CT features were compared to hearing, age, collagen mutations, and bone mineral density. Results: Unexpectedly a high prevalence of footplate, ossicle, and otic capsule anomalies was observed, even in NH ears. Footplate hypodensity or thickening was mostly found in ears without conductive hearing loss. There were significantly more retrofenestral anomalies or window obstruction in ears with a sensorineural hearing loss component than in ears without. Age was significantly higher in ears with middle layer hypodensity than in ears without. Patients with mutations were expected to have reduced collagen quantity and had significantly more footplate or retrofenestral anomalies than those with qualitative mutations. BMD was significantly higher in ears without temporal hyperpneumatization. Conclusion: Temporal bone CT features in OI are present in a large proportion of patients, had they hearing loss or not, and might be determined more by collagen mutation type than by age or BMD.
One-Year Outcome of Intravitreal Tissue Plasminogen Activator, Ranibizumab, and Gas Injections for Submacular Hemorrhage in Polypoidal Choroidal Vasculopathy
Authors: Kitagawa Yorihisa , Shimada Hiroyuki , Mori Ryusaburo , Tanaka Koji , Wakatsuki Yu , Onoe Hajime , Kaneko Hiroyuki , Machida Yumiko , Nakashizuka Hiroyuki
Published: April 2022

This study investigated one-year outcomes of treatment with one session of intravitreal recombinant tissue plasminogen activator, ranibizumab, and gas injections for submacular hemorrhage secondary to polypoidal choroidal vasculopathy (PCV). An extended study of a previous prospective trial of this treatment modality in PCV patients was conducted in 64 patients (64 eyes). Early Treatment Diabetic Retinopathy Study (ETDRS) score, central retinal thickness (CRT), and central pigment epithelial detachment thickness (CPEDT) before and 1, 3, and 12 months after treatment were analyzed. Mean ETDRS score increased from 58 at baseline to 64 letters (p = 0.0122), CRT decreased from 543 to 192 μm (p < 0.0001), and CPEDT decreased from 161 to 103 μm (p = 0.0668) at 3 months and were maintained until 12 months. Complications requiring reoperation occurred within one month in four eyes. Recurrence was observed in 46 eyes (72%), and 1.6 ± 1.5 (0–7) intravitreal aflibercept injections were given pro re nata. Univariate and multivariate analyses identified CPEDT as the pre- and post-treatment factor affecting 12-month ETDRS score (p < 0.0001). Improved visual acuity stabilized 3 months after treatment. Although 72% of patients experienced recurrence, an average of 1.6 aflibercept injections/patient maintained visual acuity up to 12 months. CPEDT was the most important factor associated with visual outcome.
Isolation and Characterisation of Quercitrin as a Potent Anti-Sickle Cell Anaemia Agent from Alchornea cordifolia
Authors: Adeniyi Olayemi , Baptista Rafael , Bhowmick Sumana , Cookson Alan , Nash Robert J. , Winters Ana , Shen Jianying , Mur Luis A. J.
Published: April 2022

Alchornea cordifolia Müll. Arg. (commonly known as Christmas Bush) has been used traditionally in Africa to treat sickle cell anaemia (a recessive disease, arising from the S haemoglobin (Hb) allele), but the active compounds are yet to be identified. Herein, we describe the use of sequential fractionation coupled with in vitro anti-sickling assays to purify the active component. Sickling was induced in HbSS genotype blood samples using sodium metabisulphite (Na2S2O5) or through incubation in 100% N2. Methanol extracts of A. cordifolia leaves and its sub-fractions showed >70% suppression of HbSS erythrocyte sickling. The purified compound demonstrated a 87.2 ± 2.39% significant anti-sickling activity and 93.1 ± 2.69% erythrocyte sickling-inhibition at 0.4 mg/mL. Nuclear magnetic resonance (NMR) spectra and high-resolution mass spectroscopy identified it as quercitrin (quercetin 3-rhamnoside). Purified quercitrin also inhibited the polymerisation of isolated HbS and stabilized sickle erythrocytes membranes. Metabolomic comparisons of blood samples using flow-infusion electrospray-high resolution mass spectrometry indicated that quercitrin could convert HbSS erythrocyte metabolomes to be like HbAA. Sickling was associated with changes in antioxidants, anaerobic bioenergy, and arachidonic acid metabolism, all of which were reversed by quercitrin. The findings described could inform efforts directed to the development of an anti-sickling drug or quality control assessments of A. cordifolia preparations.
Necrotizing Fasciitis of the Upper Limb: Optimizing Management to Reduce Complications
Authors: La Padula Simone , Pensato Rosita , Zaffiro Antonio , Hermeziu Oana , D’Andrea Francesco , Pizza Chiara , Meningaud Jean Paul , Hersant Barbara
Published: April 2022

Background: Necrotizing fasciitis (NF) is a severe, potentially life-threatening condition. The aim of this study is to identify strategies aimed at reducing complications in patients with NF of the upper limb. Methods: We conducted a retrospective study on patients admitted to our Unit for suspected NF of the upper limb. The analyzed data included patient characteristics, delay before primary care, clinical and biological signs upon arrival, pathogens involved, and the rate of amputations and mortality. Results: A total of 21 patients presented with confirmed necrotizing bacterial dermohypodermitis-NBDH with NF (NBDH-NF) affecting the upper limb. The mean delay between the onset of symptoms and the clinical examination in the Emergency Dermatology Unit was 48 h (range: 6 to 72 h). The mean delay between admission and primary surgery was 150 min (range: 60 min to 280 min). No amputations were performed. All patients were alive one year after the first surgical procedure. Conclusions: Our study demonstrated that it is possible to reduce mortality and morbidity rates in NF of the upper limb. Timely diagnosis and early treatment and a multidisciplinary medico-surgical dedicated team providing care can significantly modify the outcomes. Early surgical debridement is the most important factor affecting the prognosis of these infections.
Unique and Repeated Stwintrons (Spliceosomal Twin Introns) in the Hypoxylaceae
Authors: Fekete Erzsébet , Pénzes Fruzsina , Ág Norbert , Ág-Rácz Viktória , Sándor Erzsébet , Scazzocchio Claudio , Flipphi Michel , Karaffa Levente
Published: April 2022

Introns are usually non-coding sequences interrupting open reading frames in pre-mRNAs [D1,2]. Stwintrons are nested spliceosomal introns, where an internal intron splits a second donor sequence into two consecutive splicing reactions leading to mature mRNA. In Hypoxylon sp. CO27-5, 36 highly sequence-similar [D1,2] stwintrons are extant (sister stwintrons). An additional 81 [D1,2] sequence-unrelated stwintrons are described here. Most of them are located at conserved gene positions rooted deep in the Hypoxylaceae. Absence of exonic sequence bias at the exon–stwintron junctions and a very similar phase distribution were noted for both groups. The presence of an underlying sequence symmetry in all 117 stwintrons was striking. This symmetry, more pronounced near the termini of most of the full-length sister stwintrons, may lead to a secondary structure that brings into close proximity the most distal splice sites, the donor of the internal and the acceptor of the external intron. The Hypoxylon stwintrons were overwhelmingly excised by consecutive splicing reactions precisely removing the whole intervening sequence, whereas one excision involving the distal splice sites led to a frameshift. Alternative (mis)splicing took place for both sister and uniquely occurring stwintrons. The extraordinary symmetry of the sister stwintrons thus seems dispensable for the infrequent, direct utilisation of the distal splice sites.
Pulmonary Lymphangitis Poses a Major Challenge for Radiologists in an Oncological Setting during the COVID-19 Pandemic
Authors: Fusco Roberta , Simonetti Igino , Ianniello Stefania , Villanacci Alberta , Grassi Francesca , Dell’Aversana Federica , Grassi Roberta , Cozzi Diletta , Bicci Eleonora , Palumbo Pierpaolo , Borgheresi Alessandra , Giovagnoni Andrea , Miele Vittorio , Barile Antonio , Granata Vincenza
Published: April 2022

Due to the increasing number of COVID-19-infected and vaccinated individuals, radiologists continue to see patients with COVID-19 pneumonitis and recall pneumonitis, which could result in additional workups and false-positive results. Moreover, cancer patients undergoing immunotherapy may show therapy-related pneumonitis during imaging management. This is otherwise known as immune checkpoint inhibitor-related pneumonitis. Following on from this background, radiologists should seek to know their patients’ COVID-19 infection and vaccination history. Knowing the imaging features related to COVID-19 infection and vaccination is critical to avoiding misleading results and alarmism in patients and clinicians.
Integrating IPAT and CLUMondo Models to Assess the Impact of Carbon Peak on Land Use
Authors: Wang Han , Jin Yujie , Hong Xingming , Tian Fuan , Wu Jianxian , Nie Xin
Published: April 2022

China’s growth plans include a carbon emission peak policy, which is a restriction that indirectly impacts land use structure. In this study, we simulate different paths for achieving policy objectives, and explore the linkages between those paths and land use change. The IPAT model was used to simulate the carbon emissions generated from a natural development scenario, an ideal policy scenario, and a retributive carbon emission scenario in China from 2020 to 2030. The simulation results were incorporated into the CLUMondo model as a demand driver to simulate the land use change in 2030. The results show that carbon emission peak policy can somewhat reduce carbon emissions and increase building land in a regulated way. However, the policy may also lead to a short-term surge in carbon emissions, a reactive expansion of arable land and building land. This may reduce losses in economic development when carbon emissions are limited, but does not achieve the integration of social, economic, and ecological goals. This study links the carbon emission peak policy with land use change and provides a fresh perspective on the Chinese government’s carbon reduction policy.
The UN Global Compacts and the Common European Asylum System: Coherence or Friction?
Authors: Guild Elspeth , Allinson Kathryn , Busuttil Nicolette
Published: April 2022

This paper examines the “protective potential” of the Global Compacts on Refugees and Migrants vis à vis existing commitments to fundamental rights within the European Union (EU). The relationship between the two normative frameworks is scrutinised to establish the extent to which the two might be mutually supportive or contradictory, since this determines the Compacts’ capacity to inform the interpretation of EU fundamental rights within the Common European Asylum System (CEAS). This paper explores this protective potential through three of the Compacts’ key guiding principles: respect for human rights and the rule of law, the principle of non-regression, and the principle of non-discrimination. The Compacts’ commitments to the first two are presented as sites of coherence where the Compacts concretely express pre-existing protections within EU law and provide a blueprint for implementation in the migration sphere. However, the Compacts’ principle of non-discrimination reveals an area of friction with EU primary law. It is argued that the implementation of this principle can address the inherently discriminatory system underpinning EU law. Within the EU, rather than undermining international and national human rights obligations, the Compacts present an opportunity to refine the implementation of existing EU fundamental rights obligations applicable to migrants and refugees.
Quercetin Abrogates Oxidative Neurotoxicity Induced by Silver Nanoparticles in Wistar Rats
Authors: Elblehi Samar S. , Abd El-Maksoud Eman M. , Aldhahrani Adil , Alotaibi Saqer S. , Ghamry Heba I. , Elgendy Salwa A. , Soliman Mohamed Mohamed , Shukry Mustafa
Published: April 2022

This study aimed to investigate the oxidative neurotoxicity induced by silver nanoparticles (AgNPs) and assess the neuroprotective effects of quercetin against this toxicity. Forty adult male rats were divided into four equal groups: control, AgNPs (50 mg/kg intraperitoneally), quercetin (50 mg/kg orally), and quercetin + AgNPs. After 30 days, blood and brain tissue samples were collected for further studies. AgNP exposure increased lipid peroxidation and decreased glutathione peroxidase, catalase, and superoxide dismutase activities in brain tissue. AgNPs decreased serum acetylcholine esterase activity and γ-aminobutyric acid concentrations. AgNPs upregulated tumor necrosis factor-α, interleukin-1β, and Bax transcript levels. AgNPs reduced the transcripts of claudin-5, brain-derived neurotrophic factor, paraoxonase, nuclear factor-erythroid factor 2 (Nrf2), and Bcl-2. Histopathologically, AgNPs caused various degenerative changes and neuronal necrosis associated with glial cell reactions. AgNPs increased the immunohistochemical staining of glial fibrillary acidic protein (GFAP) in the cerebrum and cerebellum. Oral treatment with quercetin efficiently counteracted the opposing effects of AgNPs on brain tissue via modulation of tight junction proteins, Nrf2, and paraoxonase, and its positive mechanism in modulating pro-inflammatory cytokines and the downregulation of GFAP expression, and the apoptotic pathway. AgNPs also altered the severity of histopathological lesions and modulated GFAP immunostaining in the examined tissue.
Prognostic Impact and Predictors of New-Onset Atrial Fibrillation in Heart Failure
Authors: Choi Hyo-In , Lee Sang Eun , Kim Min-Seok , Lee Hae-Young , Cho Hyun-Jai , Choi Jin Oh , Jeon Eun-Seok , Hwang Kyung-Kuk , Chae Shung Chull , Baek Sang Hong , Kang Seok-Min , Choi Dong-Ju , Yoo Byung-Su , Kim Kye Hun , Cho Myeong-Chan , Oh Byung-Hee , Kim Jae-Joong
Published: April 2022

Background: The prognostic impact and predictors of NOAF in HF patients are not fully elucidated. This study aims to determine whether new-onset atrial fibrillation (NOAF) affects patient outcome and investigate predictors of atrial fibrillation (AF) in acute heart failure (HF) patients using real-world data. Methods: The factors associated with NOAF in 2894 patients with sinus rhythm (SR) enrolled in the Korean Acute Heart Failure (KorAHF) registry were investigated. Survival was analyzed using AF as a time-dependent covariate. Relevant predictors of NOAF were analyzed using multivariate proportional hazards models. Results: Over 27.4 months, 187 patients developed AF. The median overall survival time was over 48 and 9.9 months for the SR and NOAF groups, respectively. Cox regression analysis with NOAF as a time-dependent covariate showed a higher risk of death among patients with NOAF. Multivariate Cox modeling showed that age, worsening HF, valvular heart disease (VHD), loop diuretics, lower heart rate, larger left atrium (LA) diameter, and elevated creatinine levels were independently associated with NOAF. Risk score indicated the number of independent predictors. The incidence of NOAF was 2.9%, 9.4%, and 21.8% in the low-risk, moderate-risk, and high-risk groups, respectively (p < 0.001). Conditional inference tree analysis identified worsening HF, heart rate, age, LA diameter, and VHD as discriminators. Conclusions: NOAF was associated with decreased survival in acute HF patients with SR. Age, worsening HF, VHD, loop diuretics, lower heart rate, larger LA diameter, and elevated creatinine could independently predict NOAF. This may be useful to risk-stratify HF patients at risk for AF.
Evaluation of Chinese Natural Language Processing System Based on Metamorphic Testing
Authors: Jin Lingzi , Ding Zuohua , Zhou Huihui
Published: April 2022

A natural language processing system can realize effective communication between human and computer with natural language. Because its evaluation method relies on a large amount of labeled data and human judgment, the question of how to systematically evaluate its quality is still a challenging task. In this article, we use metamorphic testing technology to evaluate natural language processing systems from the user’s perspective to help users better understand the functionalities of these systems and then select the appropriate natural language processing system according to their specific needs. We have defined three metamorphic relation patterns. These metamorphic relation patterns respectively focus on some characteristics of different aspects of natural language processing. Moreover, on this basis, we defined seven metamorphic relations and chose three tasks (text similarity, text summarization, and text classification) to evaluate the quality of the system. Chinese is used as target language. We extended the defined abstract metamorphic relations to these tasks, and seven specific metamorphic relations were generated for each task. Then, we judged whether the metamorphic relations were satisfied for each task, and used them to evaluate the quality and robustness of the natural language processing system without reference output. We further applied the metamorphic test to three mainstream natural language processing systems (including BaiduCloud API, AliCloud API, and TencentCloud API), and on the PWAS-X datasets, LCSTS datasets, and THUCNews datasets. Experiments were carried out, revealing the advantages and disadvantages of each system. These results further show that the metamorphic test can effectively test the natural language processing system without annotated data.
Edge of Chaos in Memristor Cellular Nonlinear Networks
Authors: Slavova Angela , Ignatov Ventsislav
Published: April 2022

Information processing in the brain takes place in a dense network of neurons connected through synapses. The collaborative work between these two components (Synapses and Neurons) allows for basic brain functions such as learning and memorization. The so-called von Neumann bottleneck, which limits the information processing capability of conventional systems, can be overcome by the efficient emulation of these computational concepts. To this end, mimicking the neuronal architectures with silicon-based circuits, on which neuromorphic engineering is based, is accompanied by the development of new devices with neuromorphic functionalities. We shall study different memristor cellular nonlinear networks models. The rigorous mathematical analysis will be presented based on local activity theory, and the edge of chaos domain will be determined in the models under consideration. Simulations of these models working on the edge of chaos will show the generation of static and dynamic patterns.
Robust Parametric Identification for ARMAX Models with Non-Gaussian and Coloured Noise: A Survey
Authors: Escobar Jesica , Poznyak Alexander
Published: April 2022

In this paper the Cramer-Rao information bound for ARMAX (Auto-Regression-Moving-Average-Models-with-Exogenuos-inputs) under non-Gaussian noise is derived. It is shown that the direct application of the Least Squares Method (LSM) leads to incorrect (shifted) parameter estimates. This inconsistency can be corrected by the implementation of the parallel usage of the MLMW (Maximum Likelihood Method with Whitening) procedure, applied to all measurable variables of the model, and a nonlinear residual transformation using the information on the distribution density of a non-Gaussian noise, participating in Moving Average structure. The design of the corresponding parameter-estimator, realizing the suggested MLMW-procedure is discussed in details. It is shown that this method is asymptotically optimal, that is, reaches this information bound. If the noise distribution belongs to some given class, then the Huber approach (min-max version of MLM) may be effectively applied. A numerical example illustrates the suggested approach.
New Equipment and Method for Refining the Solidified Grain Structure
Authors: Rónaföldi Arnold , Veres Zsolt , Svéda Mária , Roósz András
Published: April 2022

The mechanical properties of solidified alloys strongly depend on the grain size. In many practical cases at the given solidification parameters (temperature gradient and solid/liquid interface velocity), the solidified microstructure is columnar, meaning that the mechanical properties differ depending on the direction, which results in the material being unsuitable for application. The microstructure can be changed from columnar to equiaxed through the inclusion of grain refinement material. This strategy is well known in the literature as the columnar/equiaxed transition (CET). In some cases, it is beneficial if the CET can be produced without using grain refinement material; for example, it may detrimentally affect the mechanical properties (such as when the Al alloy ingot is used in pressing). The stirring of the melt as an alternative for the use of grain refinement material could solve this problem as intensive melt flow can break some particles from growing dendrites. This paper demonstrates a new type of traveling magnetic field inductor that is used to produce strong shearing stress in the flow perpendicular to the solidification front by causing part of the metallic melt layers touching each other to flow in an opposite direction. Through some examples, we demonstrate the effect of stirring by the new inductor on the solidified grain structure.
Fatigue Behavior of Alloy Steels Sintered from Pre-Alloyed and Diffusion-Bonding Alloyed Powders
Authors: Tan Zhaoqiang , Liu Yong , Huang Xiaolin , Li Songlin
Published: April 2022

Porosity and phases are considered to be two key factors for the fatigue performance of powder metallurgy steels. In this paper, the fatigue strengths of the alloy steels sintered from two typical types of powders, pre-alloyed Fe-Cr-Mo (Astaloy CrM), and diffusion-bonding alloyed Fe-Cu-Mo-Ni (Distaloy AE), were comparatively analyzed in view of the geometry of porosity, the phases constitution, and fractography of fracture. Different modes of fatigue fracture were distinguished between the two materials. Namely, a trans-particle fracture is predominant in the Disitaloy AE steel due to the heterogeneous phases which consist of soft phases in powder interior and hard phases along powder borders. In contrast, the fatigue fracture of the Astaloy CrM steel with a homogeneous mono-phase of martensite is characterized by an inter-particle fracture at the sintering necks. Moreover, the fatigue endurance limit of the Distaloy AE steel was not pronouncedly improved by increasing sintering temperature in comparison with the Astaloy CrM steel. This was attributed to the softening of the network constructed by martensite at sintering necks. A modified Murakami model which considers micro-scale defect and micro-hardness is effective to predict the fatigue performance of the alloy steels sintered from pre-alloyed and diffusion-bonding alloyed powders, respectively.
Fabrication of an Oscillating Thermocycler to Analyze the Canine Distemper Virus by Utilizing Reverse Transcription Polymerase Chain Reaction
Authors: Chen Jyh-Jian , Lin Zong-Hong
Published: April 2022

The reverse transcription-polymerase chain reaction (RT-PCR) has been utilized as an effective tool to diagnose the infectious diseases of viruses. In the present work, the oscillating thermocycler is fabricated and performed to carry out the one-step RT-PCR process successfully. The ribonucleic acid (RNA) mixture is pipetted into the fixed sample volume inside an aluminum reaction block. The sample oscillates the pathway onto the linear motion control system and through the specific RT-PCR heating zones with individual homemade thermal control modules. The present oscillating thermocycler combines the merits of the chamber type and the CF type systems. Before PCR, the reaction chamber moves to the low-temperature zone to complete the RT stage and synthesize the complementary deoxyribonucleic acid (DNA). Next, the low-temperature zone is regulated to the annealing zone. Furthermore, the reactive sample is moved back and forth among three isothermal zones to complete PCR. No extra heating zone is required for the RT stage. The total length of the moving displacement of the chamber is within 100 mm. The miniaturization of the oscillating thermocycler can be expected. In our oscillatory device, the denaturation zone located between the annealing and extension zones is suggested as the appropriate arrangement of the heating blocks. Heat management without thermal cross-talk is easy. Finally, an improved oscillating device is demonstrated to execute the RT-PCR process directly, utilized to amplify the canine distemper virus templates successfully, which could be well applied to a low-cost DNA analysis system in the future.
An Evaluation of MEMS-IMU Performance on the Absolute Trajectory Error of Visual-Inertial Navigation System
Authors: Liu Yunfei , Li Zhitian , Zheng Shuaikang , Cai Pengcheng , Zou Xudong
Published: April 2022

Nowadays, accurate and robust localization is preliminary for achieving a high autonomy for robots and emerging applications. More and more, sensors are fused to guarantee these requirements. A lot of related work has been developed, such as visual-inertial odometry (VIO). In this research, benefiting from the complementary sensing capabilities of IMU and cameras, many problems have been solved. However, few of them pay attention to the impact of different performance IMU on the accuracy of sensor fusion. When faced with actual scenarios, especially in the case of massive hardware deployment, there is the question of how to choose an IMU appropriately? In this paper, we chose six representative IMUs with different performances from consumer-grade to tactical grade for exploring. According to the final performance of VIO based on different IMUs in different scenarios, we analyzed the absolute trajectory error of Visual-Inertial Systems (VINS_Fusion). The assistance of IMU can improve the accuracy of multi-sensor fusion, but the improvement of fusion accuracy with different grade MEMS-IMU is not very significant in the eight experimental scenarios; the consumer-grade IMU can also have an excellent result. In addition, the IMU with low noise is more versatile and stable in various scenarios. The results build the route for the development of Inertial Navigation System (INS) fusion with visual odometry and at the same time, provide a guideline for the selection of IMU.
Vacuum and Low-Temperature Characteristics of Silicon Oxynitride-Based Bipolar RRAM
Authors: Das Nayan C. , Kim Minjae , Hong Sung-Min , Jang Jae-Hyung
Published: April 2022

This study investigates the switching characteristics of the silicon oxynitride (SiOxNy)-based bipolar resistive random-access memory (RRAM) devices at different operating ambiances at temperatures ranging from 300 K to 77 K. The operating ambiances (open air or vacuum) and temperature affect the device’s performance. The electroforming-free multilevel bipolar Au/Ni/SiOxNy/p+-Si RRAM device (in open-air) becomes bilevel in a vacuum with an on/off ratio >104 and promising data retention properties. The device becomes more resistive with cryogenic temperatures. The experimental results indicate that the presence and absence of moisture (hydrogen and hydroxyl groups) in open air and vacuum, respectively, alter the elemental composition of the amorphous SiOxNy active layer and Ni/SiOxNy interface region. Consequently, this affects the overall device performance. Filament-type resistive switching and trap-controlled space charge limited conduction (SCLC) mechanisms in the bulk SiOxNy layer are confirmed.
Chemical Diversity of Teeth and Bone Fragments from a Newly Discovered Upper Muschelkalk Bone Bed from Silesia, Poland
Authors: Krzykawski Tomasz , Szopa Krzysztof , Niedźwiedzki Robert , Setkiewicz Krzysztof , Czaja Maria
Published: April 2022

The new exposure of the Upper Muschelkalk clays and dolomites located south of Kalety (Tarnogórski District, Silesia, Poland) provided numerous remains of vertebrates represented by teeth, scales, long bones, and coprolites. Despite the influence of hydrothermal processes leading to dolomitization and Zn-Pb deposit formation, the preservation of fossil remains is good. The taxonomic diversity and accumulation of vertebrate debris in the dolomite are similar to other “bone beds” from the Muschelkalk and the Lower Keuper units. The SEM-EDS, EMP-WDS, and XRD analyses confirm that the examined remains consist of hydroxylapatite containing carbonate ions. Most vertebrate teeth as well as some bone fragments show zoning in the BSE imaging. In tooth cross-sections, three or two zones are preserved: (I) the outermost zone, associated with diagenetic mineralization of enameloid apatite, (II) a intermediate zone (orthodentine), and (III) the most porous internal zone (osteodentine). Decreasing P, Ca, Sr in the composition of the apatite which forms successive zones, is visible from the most external to the central part. Selective diagenetic substitution and adsorption of some elements by apatite crystals can allow recognition of the genetic origin of highly damaged or transported fragments scattered in the sedimentary layers. The chemical behavior of bioapatite, from deposition to digenesis, shows its useful role for identification of the formation process and potential, younger changes (e.g., hydrothermal overprint). The X-ray diffraction data, particularly cell parameters “a” and “c”, can determine the degree of crystallinity and/or diagenesis. Moreover, correlation between some elements/ions (e.g., Sr, Ba, Ca, Mg, F, OH) can be helpful for the identification of the fossil type, especially if the bones are small and incomplete.
Characterization of Bentonites from the In Situ ABM5 Heater Experiment at Äspö Hard Rock Laboratory, Sweden
Authors: Fernández Ana María , Marco José F. , Nieto Paula , León Fco. Javier , Robredo Luz María , Clavero María Ángeles , Cardona Ana Isabel , Fernández Sergio , Svensson Daniel , Sellin Patrik
Published: April 2022

The Alternative Buffer Material ABM5 experiment is an in situ medium-scale experiment performed at Äspö Hard Rock Laboratory (HRL) conducted by SKB in Sweden with the aim of analysing the long-term stability of bentonites used as an engineering barrier for a high-level radioactive waste repository (HLWR). In this work, four different ring-shaped Ca- and Na-bentonite blocks, which were piled around a carbon steel cylindrical heater, subjected to a maximum temperature of 250 °C and hydrated with saline Na-Ca-Cl Äspö groundwater (0.91 ionic strength), were characterized after dismantling. This work allowed us to identify the main geochemical processes involved, as well as the modifications in the physico-chemical properties and pore water composition after 4.4 years of treatment. No significant modifications in mineralogy were observed in samples close to the heater contact, except an increase in Fe content due to C-steel corrosion, carbonate dissolution/precipitation (mainly calcite and siderite) and Mg increase. No magnetite and a low amount of Fe(II) inside the clay mineral structure were detected. No modifications were observed in the smectite structure, except a slight increase in total and tetrahedral charge. A decrease in external surface area and cation exchange capacity (CEC) was found in all samples, with lower values being detected at the heater contact. As a consequence of the diffusion of the infiltrating groundwater, a modification of the composition at clay mineral exchange sites occurred. Ca-bentonites increased their Na content at exchange sites, whereas Na-bentonite increased their Ca content. Exchangeable Mg content decreased in all bentonites, except in MX-80 located at the bottom part of the package. A salinity gradient is observed through the bentonite blocks from the granite to the heater contact due to anions are controlled by diffusion and anion exclusion. The pore water chemistry of bentonites evolved as a function of the diffusion transport of the groundwater, the chemical equilibrium of cations at exchange sites and mineral dissolution/precipitation processes. These reactions are in turn dependent on temperature and water vapor fluxes.
Study on Grinding Additives in Cassiterite–Polymetallic Sulfide Ore Grinding
Authors: Yang Jinlin , Ma Shaojian , Zhou Wentao , Zhu Pengyan
Published: April 2022

To attempt a new approach to improve the grinding of cassiterite–polymetallic sulfide ores while simultaneously reducing cassiterite overgrinding and sulfide undergrinding, this article looked into the effects of grinding chemical additives on the distribution of grinding product size. Six chemicals, namely sodium hexametaphosphate, triethanolamine, ferric sulphate, aluminum chloride, polyaluminum chloride and polyacrylamide, were compared in terms of their influence on the grinding product size distribution. The results showed that the six chemicals changed the distribution results with varying orientations and degrees and that the addition of polyacrylamide achieved the most satisfactory effect by decreasing the production of both coarse and fine size fractions and increasing the production of qualified particles. The effect of the molecular weight of polyacrylamide on the grinding was also discussed. The polyacrylamides with molecular weights of about 3 × 106, 5 × 106, 8 × 106 and 12 × 106 could help to produce less of the coarse size fraction and more of the qualified size fraction, but only the polyacrylamides with molecular weights of 3 × 106 and 5 × 106 produced pronounced changes. Moreover, the polyacrylamides could slightly reduce the production of the fine size fraction. Polyacrylamide with a 5 × 106 molecular weight was better than that with a 3 × 106 molecular weight in aiding the grinding of the discussed ore. It was also found that the aid action of the polyacrylamide with a 5 × 106 molecular weight was related to grinding concentration and that a low grinding concentration of less than 70% solid mass was helpful in exerting its aid action. Using polyacrylamide could shorten the grinding time that is needed to achieve the same, or even improved, product size distribution.
Visualization and Comprehension of Electronic and Topographic Contrasts on Cooperatively Switched Diarylethene-Bridged Ditopic Ligand
Authors: Hnid Imen , Guan Lihao , Chatir Elarbi , Cobo Saioa , Lafolet Frédéric , Maurel François , Lacroix Jean-Christophe , Sun Xiaonan
Published: April 2022

Diarylethene is a prototypical molecular switch that can be reversibly photoisomerized between its open and closed forms. Ligands bpy-DAE-bpy, consisting of a phenyl-diarylethene-phenyl (DAE) central core and bipyridine (bpy) terminal substituents, are able to self-organize. They are investigated by scanning tunneling microscopy at the solid–liquid interface. Upon light irradiation, cooperative photochromic switching of the ligands is recognized down to the submolecular level. The closed isomers show different electron density of states (DOS) contrasts, attributed to the HOMO or LUMO molecular orbitals observed. More importantly, the LUMO images show remarkable differences between the open and closed isomers, attributed to combined topographic and electronic contrasts mainly on the DAE moieties. The electronic contrasts from multiple HOMO or LUMO distributions, combined with topographic distortion of the open or closed DAE, are interpreted by density functional theory (DFT) calculations.
Gas Sensors and Semiconductor Nanotechnology
Authors: Mizsei János
Published: April 2022

Solid-state semiconductor gas sensors have been attracting a great deal of attention for over two decades, due to their importance in gas analysis and safety applications [...]
Size Effects in Single- and Few-Layer MoS2 Nanoflakes: Impact on Raman Phonons and Photoluminescence
Authors: Cortijo-Campos Sandra , Prieto Carlos , De Andrés Alicia
Published: April 2022

The high optical absorption and emission of bidimensional MoS2 are fundamental properties for optoelectronic and biodetection applications and the opportunity to retain these properties in high quality nano-sized flakes would bring further possibilities. Here, a large set of single-layer and few-layer (2–3 layers) MoS2 flakes with size in the range from 10 nm to 20 μm are obtained on sapphire by vapor deposition techniques and evaluated combining the information from the Raman phonons with photoluminescence (PL) and absorption bands. The flakes have triangular shape and are found to be progressively relaxed from the tensile strain imposed by the sapphire substrate as their size is reduced. An increasing hole doping as size decreases is deduced from the blue shift of the A1g phonon, related to charge transfer from adsorbed oxygen. No clear correlation is observed between defects density and size, therefore, doping would be favored by the preferential adsorption of oxygen at the edges of the flakes, being progressively more important as the edge/surface ratio is incremented. This hole doping also produces a shift of the PL band to higher energies, up to 60 meV. The PL intensity is not found to be correlated to the size but to the presence of defects. The trends with size for single-layer and for 2–3 layer samples are found to be similar and the synthesis method does not influence PL efficiency which remains high down to 40 nm being thus promising for nanoscale photonics.
Ensuring the Efficacious Iron Fortification of Foods: A Tale of Two Barriers
Authors: Hurrell Richard F.
Published: April 2022

Iron fortification of foods has always been a challenge. This is because iron fortification compounds vary widely in relative absorption; because many foods undergo unacceptable changes in color or flavor from the addition of iron; and because many of the iron-fortified foods contain potent inhibitors of iron absorption. These technical barriers have largely been overcome, and efficacious iron-fortified foods, that maintain or improve the iron status of women or children in long-term feeding studies, can be designed. Commercially fortified infant foods are efficacious, and other commercial iron-fortified foods targeted at women and children will provide a useful amount of iron provided the fortification level is adjusted according to the relative absorption of the iron compound. Technologies for the large-scale fortification of wheat and maize flour are also well established, and iron fortification of rice, using the recently developed extruded premix technique, is showing great promise. However, some important knowledge gaps still remain, and further research and development is needed in relation to iron (and iodine)-fortified salt and iron-fortified liquid milk. The usefulness of less-soluble iron compounds, such as ferrous fumarate, to fortify foods for infants and young children in low- and middle-income countries (LMICs) also needs further investigation. A more formidable barrier to efficacious iron-fortified food has been reported in recent years. This is the infection-initiated inflammation barrier, which inhibits iron absorption in response to infection. This barrier is particularly important in LMICs where infections such as malaria and HIV are widespread, and gastrointestinal infections are common due to poor quality water supplies and sanitation. Another source of inflammation in such countries is the high prevalence of obesity in women. Most countries in sub-Saharan Africa have high inflammation which not only decreases the efficacy of iron-fortified and iron-biofortified foods but complicates the monitoring of large-scale iron fortification programs. This is because iron deficiency anemia cannot be differentiated from the more prominent anemia of inflammation and because inflammation confounds the measurement of iron status. There is an urgent need to better quantify the impact of inflammation on the efficacy of iron-fortified foods. However, at present, in LMICs with high inflammation exposure, infection control, cleaner water, improved sanitation, and a decrease in obesity prevalence will undoubtedly have a greater impact on iron status and anemia than the iron fortification of foods.
Synthesis and Antiproliferative Activity of Novel Imipridone–Ferrocene Hybrids with Triazole and Alkyne Linkers
Authors: Czuczi Tamás , Murányi József , Bárány Péter , Móra István , Borbély Adina , Csala Miklós , Csámpai Antal
Published: April 2022

Imipridones, including ONC201, ONC206 and ONC212 (which are emblematic members of this class of compounds developed by Oncoceutics) constitute a novel class of anticancer agents, with promising results in clinical trials. With the aim of increasing the ROS (reactive oxygen species) responsivity of the synthesized molecules, a set of novel ferrocene–imipridone hybrids were designed and synthesized. Our strategy was motivated by the documented interplay between the imipridone-triggered activation of TRAIL (the tumor necrosis factor-related apoptosis-inducing ligand) and mitochondrial ClpP (Caseinolytic protease P) and the ROS-mediated effect of ferrocene-containing compounds. In order to obtain novel hybrids with multitarget characters, the ferrocene moiety was tethered to the imipridone scaffold through ethynylene and 1,2,3-triazolyl linkers by using Sonogashira coupling of Cu(I)- and Ru(II)-catalyzed azide–alkyne cycloadditions. The biological activities of the new hybrids were examined by using in vitro cell viability assays on four malignant cell lines (PANC-1, A2058, EBC-1 and Fadu), along with colony formation assays on the most resistant PANC-1 cell line. Several hybrids caused a significantly greater drop in the cell viability compared to ONC201, and two of them completely overcame the resistance, with IC50 values comparable to those produced by ONC201. The two most potent hybrids, but not ONC201, induced apoptosis/necrosis in PANC-1 and A2058 cells after 24 h of treatment.
Iron Oxide Nanoparticles-Plant Insignia Synthesis with Favorable Biomedical Activities and Less Toxicity, in the “Era of the-Green”: A Systematic Review
Authors: Hamdy Nadia M. , Boseila Amira A. , Ramadan Ahmed , Basalious Emad B.
Published: April 2022

In the era of favoring environment-friendly approaches for pharmaceutical synthesis, “green synthesis” is expanding. Green-based nanomedicine (NM), being less toxic and if having biomedical acceptable activities, thence, the chemical methods of synthesis are to be replaced by plants for reductive synthesis. Iron oxide nanoparticles (IONPs) exhibited remarkable anti-microbial and anti-cancer properties, besides being a drug delivery tool. However, owing to limitations related to the chemical synthetic method, plant-mediated green synthesis has been recognized as a promising alternative synthetic method. This systematic review (SR) is addressing plant-based IONPs green synthesis, characteristics, and toxicity studies as well as their potential biomedical applications. Furthermore, the plant-based green-synthesized IONPs in comparison to nanoparticles (NPs) synthesized via other conventional methods, characteristics, and efficacy or toxicity profiles would be mentioned (if available). Search strategy design utilized electronic databases including Science Direct, PubMed, and Google Scholar search. Selection criteria included recent clinical studies, available in the English language, published till PROSPERO registration. After screening articles obtained by first electronic database search, by title, abstract and applying the PICO criteria, the search results yielded a total of 453 articles. After further full text filtrations only 48 articles were included. In conclusion, the current SR emphasizes the perspective of the IONPs plant-mediated green synthesis advantage(s) when utilized in the biomedical pharmaceutical field, with less toxicity.