bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an RP-HPLC Method for the Simultaneous Quantification of Encorafenib and Cetuximab in their Bulk Form
Authors: Annapurna Mukthinuthalapati M , DK Shanthi Priya
Published: July 2024

The primary goal of this investigation was to develop and validate a high-performance liquid chromatograophy (HPLC) method that is straightforward, reliable, sensitive, and accurate in quantifying the concentrations of encorafenib and cetuximab in bulk form. A Waters HPLC system with an autosampler and a PDA detector was used to create the HPLC technique. With a particle size of 5 μ and dimensions of 250 x 4.6 mm, a Waters X-Terra RP-18 column was used for the separation. A combination of acetonitrile and 0.1% triethylamine (TEA) at a pH of 2.5 makes up the mobile phase. Acetonitrile to TEA is 40:60 in ratio. A 240 nm wavelength, a 10 μL injection volume, and a flow rate of 1.0 mL/min were used for the chromatographic procedure. Analyte serial dilutions will be evaluated in order to establish linearity. The stock solutions with concentrations ranging from 1.25 to 7.50 μg/mL for cetuximab and 18.75 to 112.50 μg/mL for encorafenib were used to generate these dilutions. The dilutions were made by adding the mobile phase to the stock solutions to create a series. Encorafenib and cetuximab were found to have limit of detection (LoD) and limit of quantitation (LoQ) values of 0.225 and 0.015 g/mL and 0.75 and 0.05 g/mL, respectively. The method that was developed showed a high level of accuracy, as evidenced by the relative standard deviation (RSD) being less than 2%. The developed analytical method for estimating encorafenib and cetuximab is both straightforward and accurate.
A UPLC Method for Simultaneous Estimation of Trastuzumab and Hyaluronidase in Bulk and Pharmaceutical Dosage Form
Authors: Sahithi Alapati , Choppala Asha Deepthi
Published: July 2024

Objective: The proposed research aimed at establishing a quick, accurate, and exact ultra-performance liquid chromatography (UPLC) methodology for contemporaneously analyzing trastuzumab and hyaluronidas in bulk and pharmaceutical formulation, with a focus on the stability-indicating properties of the assay. Method: Using a Water X-Bridge C18 column (50 mm x 4.6 mm x 2.5 μ) and a mobile phase of ACN: Buffer (pH 2.5) [50% v/v], pushed at 0.5 mL/min, compounds were separated chromatographically. An ultraviolet (UV) detector fixed at 260 nm was employed to identify the isolated compounds. Results: The findings showed that 1.3529 and 2.404 minutes were optimal for separating Trastuzumab and Hyaluronidase, correspondingly. The current procedure has been verified in accordance with ICH standards Q2 R1, and stability-indicating tests have been performed in accordance with ICH standards Q1A R2. Both the intra- and inter-day precisions were determined to be satisfactory. The suggested approach showed linearity between 30 to 180 μg/mL of trastuzumab and between 12.50-75μg/ mL of hyaluronidase. It was determined that the limit of detection (LoD) and limit of quantitation (LoQ) for trastuzumab were 0.36 and 1.2 μg/mL, correspondingly, whereas those for hyaluronidase were 0.15 and 0.5 μg/mL. The approach was shown to have a recovery rate around 98.6 to 99.7%. Conclusion: The suggested approach successfully separated the chemical compounds from their by-products. Therefore, trastuzumab and hyaluronidase routine evaluation and stability-indicating tests were both effectively implemented using the approach.
Development and Validation of Stability Indicating Liquid Chromatographic Method for Vandetanib: Application to Degradation Studies by LC-MS
Authors: . Asia , Nalanda RB
Published: July 2024

Objective: The central goal of this research was to formulate and validate a stability-demonstrating assay approach using reverse-phase high-performance liquid chromatography (RP-HPLC) for the assessment of vandetanib, its primary degradation products, and their respective degradation pathways, which were identified and characterized using liquid chromatography-electrophore spray ionization-mass spectrometry (LC-ESI-MS).Methods: The method was designed and developed by employing a Grace C18 column (250 x 4.6 mm x4.0 μm) and deploying a mixture of methanol and 0.1% formic acid in H2O (in a proportion of 90:10, v/v) as the mobile phase, with a flow rate maintained at 1-mL/min. The analytes were detected at a wavelength of 249 nm. Additionally, vandetanib was subjected to various stress conditions, including acidic, alkaline, oxidative, thermal, and photolytic degradation, to unearth the degradation paths for the principal degradation products.Results: The approach was meticulously formulated and validated to ensure robustness, linearity, precision, accuracy, and linear regression analysis data. A high correlation coefficient of 0.9985 was obtained in the 2 to 12 μg/mL concentration range, demonstrating a robust linear relationship. Moreover, the limit of detection (LoD) and the limit of quantification (LoQ) were found to be 0.166 and 0.503 μg/mL, respectively. In the stress degradation studies, we noticed that vandetanib degraded in alkaline and acidic mediums. The liquid chromatography-mass spectrometry (LC-MS) technique was used to characterize the degradation products.Conclusion: The devised method demonstrated itself to be quick, sensitive, precise, accurate, and robust for the vandetanib analysis, thereby facilitating its routine testing.
Estimation of Glyoxylic Acid in Emtricitabine by a New Validated RP-HPLC Method
Authors: Godela Ramreddy , Pola Kranthi Kumar , Panukanti Mukund , Kusuma Anusha , Shireen Shaik , Srinivas Martha
Published: July 2024

To establish a simple, sensitive, as well as specified method for the separation and quantitative determination of glyoxylic acid in emtricitabine by using reversed-phase high-performance liquid chromatography (RP-HPLC). A superior separation of glyoxylic acid in emtricitabine was done by using a C8 column and a mobile system of (0.1% OPA and ACN: H2O) with 1-mL/min flow rate in gradient mode and a wavelength of 210 nm. The glyoxylic acid was eluted at 5.37 minutes with good efficiency and system suitability. The method has shown good linearity ranges from a limit of detection (LoQ) to 150% level of glyoxylic acid standard solution (0.1% or 0.001 mg/mL). ICH principles validated the suggested approach, and every parameter met the requirements for the International Council of Harmonization (ICH) Q2 recognition. %RSD of System, method precision, and precision at LoQ were in the range of 0.46 to 1.91. The developed method was sensitive, specific, and precise in estimating the trace levels of glyoxylic acid in emtricitabine.
Bio-analytical Methodology for Exploring Ripretinib’s Pharmacokinetic Profile in Rat Plasma Employing Bosutinib as an Internal Standard Utilizing LC-MS/MS
Authors: Pilli Sandhya , Kalakonda Sri N , Rajendran Vijayalakshmi
Published: July 2024

A robust, consistent, straightforward, swift, and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was tailored for the bioanalytical analysis of ripretinib, with bosutinib serving as the comparative standard. This article provides a synopsis of recent developments in bioanalytical LC-MS/MS techniques utilizing a phenyl column (150x4.6 mm, 3.5 μ) and an eluting agent composed of ammonium formate at a pH of 2.5, which was altered in a 50:50 ratio with formic acid and acetonitrile. The chromatogram was utilized to analyse ripretinib, and the MS detector was equipped with electrospray ionization (ESI) method functioning in positive ionization conditions with multiple reactions monitoring (MRM). This allowed for the recording of transition m/z values of 531.4465→170.9638 for ripretinib and 511.4230 → 160.7133 for BTB. In the chromatographic analysis, ripretinib was retained for approximately 2.433 minutes, whereas BTB was retained for around 3.608 minutes. The validity of the approach extends across a linearity scale of 5 to 100 ng/mL for ripretinib, boasting a correlation index of 0.9999. In the realm of biological matrices, the extraction procedure employing the protein precipitation technique yields recovery rates of 99.6, 99.2, and 98% at the lower, middle, and higher quality control levels, respectively. The obtained results for accuracy, precision, matrix effect, and stability were determined to be within the acceptable range. To observe the investigated analyte in bodily fluids, pharmacokinetic studies yielded key parameters, including a half-life of 10 hours and a time to reach a maximum drug level of 4 hours. This thorough assessment affirms that the method satisfies rigorous criteria for system suitability and specificity well within predefined acceptance limits. Such performance positions it as an indispensable asset in the realm of bioanalysis, significantly broadening its clinical applicability.
Development and Validation of a Novel Stability-Indicating Reverse Phase High-Performance Liquid Chromatography Method for the Quantification of Capivasertib in Bulk Drug and Pharmaceutical Dosage Form
Authors: Gaur Bavita , Sahu Sandeep , Kori Mohan L
Published: July 2024

The quantification of capivasertib has been achieved using a Waters Symmetry column with UV detection at 260 nm through a novel stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method that has been established and verified. The analysis can be completed in just 5 minutes. The separation and collection of capivasertib occurred at a retention time of 3.3475 minutes. The concentration range of capivasertib was shown to be linear, ranging from 50 to 300 μg/mL. The regression equations for capivasertib were determined as y = 13485.85x + 1723.04. The detection limit for capivasertib was determined to be 0.6 μg/mL, while the quantification limits were established at 2.00 and 0.5000 μg/mL, respectively.
Promising Natural Approach: Caesalpinia bonduc Seed Extracts for Calcium Oxalate Urolithiasis in Rats
Authors: Mathapati Sunil , Kalshetti Mallinath , Kore Rudramuni
Published: July 2024

Background: It is essential to identify a therapy for hyperoxaluria that specifically focuses on reducing oxalate excretion since current therapies for urolithiasis have drawbacks. It is noteworthy to observe that the seeds of Caesalpinia bonduc (Family: Caesalpiniaceae) have been historically used by several Indian tribes for the purpose of treating renal diseases. Purpose: The primary purpose of this study was to assess the efficacy of C. bonduc seed extracts as therapeutic agents in rats with experimentally generated calcium oxalate urolithiasis. Methodology: The experimental animals were given 0.75% of ethylene glycol orally for fourteen consecutive days in order to produce calcium oxalate lithiasis. C. bonduc seed extracts, 400 mg/kg of body weight in aqueous and ethanolic forms, were administered in the same way for a further 14 days in succession. The standard antiurolithiatic medicine used was cystone, administered at a dose of 750 mg/kg of body weight. The research primarily examined the quantification of serum biochemical markers and the elimination of salt constituents from urine and renal deposits, which were shown to be challenging. Results: Ethylene glycol administered orally caused hyperoxaluria and augmented calcium, phosphate, and oxalate excretion in the kidneys. Nevertheless, the administration of C. bonduc seed extracts effectively decreased the elevated levels of oxalate in the urine, showing an inhibitory effect on the synthesis of oxalate inside the human body. The renal accumulation of components that cause stone formation in rats with calculogenic conditions decreased considerably after the administration of curative treatments with aqueous and ethanolic extracts. Discussion and Conclusion: Based on the findings, it has been shown that C. bonduc seeds have lithontriptic action, which deserves further investigation as a potential remedy for urolithiasis. Further research is necessary to elucidate the specific phytoconstituents present in seeds that are responsible for their antiurolithiatic activity.
Effect of Omega-3 Adjuvant Therapy on Cognitive Improvement and Levels of Glial Cell Line-Derived Neurotrophic Factor (GDNF) in Schizophrenia Patients Receiving Risperidone Therapy
Authors: Saidah Syamsuddin , Nurul Nadya Andi , Erlyn Limoa , Irfan Idris , Suheyra Syauki Andi , Kristian Liaury , Burhanuddin Bahar , Abdul Muis , Indrawaty Suhuyanli , Teddy Lisal Sonny
Published: July 2024

Background: Schizophrenia is a disorder with severe and persistent psychotic manifestations accompanied by cognitive impairment. Treatment with atypical antipsychotic therapy (APG-2) provides only minimal benefit for the cognitive impairment of schizophrenia patients. Many efforts are currently being expanded to find new treatments that can serve as “co-treatments” to improve neurocognition from aspects of neurobiology and neuroplasticity. Several studies found that omega-3 fatty acids can improve cognitive function in schizophrenia patients.Objective: The objective is to assess the impact of omega-3 adjuvant therapy on improving cognitive function and elevating glial cell line-derived neurotrophic factor (GDNF) levels in individuals diagnosed with schizophrenia who are undergoing risperidone treatment.Method: A research study utilizing experimental analysis, including pre-tests and post-tests with non-random group selection, was conducted at Dadi Regional Special Hospital in South Sulawesi, Indonesia, between May and July 2023. Sample testing was carried out at the HUMRC Research Laboratory. The study involved 44 participants divided into two groups: a treatment group comprising 22 individuals who received 4 mg/day risperidone along with Omega-3 supplements for eight weeks and a control group of 22 individuals who received only 4 mg/day risperidone. Cognitive function was evaluated using the MoCA-Ina tool, while GDNF serum levels were determined through enzyme-linked immunosorbent assays (ELISA). Significance was assessed through the Chi-square, Wilcoxon, Mann-Whitney, Pearson and Spearman correlation tests.Results: There was an improvement in cognitive function and a significant increase of GDNF serum levels in schizophrenia patients who received omega-3 adjuvant therapy over 8 weeks compared to individuals with schizophrenia who solely received the antipsychotic risperidone. Conclusion: Supplementing standard risperidone therapy with omega-3 adjuvant can enhance cognitive function and elevate GDNF levels.
Evaluation of Antiviral, Antibacterial and Antioxidant Activity of Morinda tinctoria Roxb., Abroma augusta L. Educes and Myristica fragrans Houtt. Ethereal Oil in Microcapsules
Authors: Verma Garima , Verma Priyanka , Srivastava Ashish , Yadav Anil K
Published: July 2024

Viruses and bacteria can interfere with human functioning; scientists are always looking for new ways to harness the healing power of plants. There is potential for a new type of medicine to be developed from plant materials rich in biologically active chemicals. The purpose of this research was to create microcapsules and scrutinize the biological outcomes of a variation of plant educes and ethereal oils. Using chromatographic techniques, we identified the primary components of the educe and ethereal oil, measured the antioxidant activity spectrophotometrically, tracked the development of 09 different infectious agents, and calculated the antiviral result on coronavirus-contaminated avian cells. The extract of Abroma augusta L. demonstrated the highest levels of antioxidant and antiviral activity (27.28 – 0.32 and 642.54 – 9.12 g TE/g dw via DPPH and ABTS techniques, correspondingly). The FRAP assay found that Morinda tinctoria Roxb. extract had the highest antioxidant activity (685.72 – 4.65 mg FS/g dw) and showed antibacterial activity against gram-positive pathogens. Excipients and their quantities were crucial to the emulsion’s consistency. The diameter of the enlarged microcapsules containing educes and ethereal oil was greater than twice in intestinal media and less than half that in gastric media, with a starting diameter of 1.86 mm.
Enhancement of Solubility by Hydrotropic Techniques of Lercanidipine HCl: In-situ Nasal Gel Development
Authors: Sanket Shinde , Swati Jagdale , Shrikant Dargude , Satish Polshettiwar
Published: July 2024

Lercanidipine HCl is a novel, third-generation, potent, vasoselective 1, 4- dihydropyridine calcium channel antagonist and a Biopharmaceutical Classification System (BCS) class II drug that aids in preventing hypertension. The final aim of the present work was to promote the solubility of lercanidipine HCl via hydrotrophy and then include it in the in-situ gel formulation. This enhancement of drug solubility was carried out by using citric acid as a hydrotropic agent. Simultaneously, using a mixture of two polymers, namely Poloxamer 407, a thermosensitive polymer with reversible thermal characteristics. Carbopol 940P is a high-viscosity builder exploited for developing in-situ gel. The optimization of preliminary batches of mixtures of both polymers was highly accepted by using 32 factorial designs. F4 was found to be highly optimized, according to all the evaluation parameters and Poloxamer 407 (18% w/v) and Carbopol 940P (0.2% w/v), with drug release of 93.23%. Goat mucosa ex-vivo investigation yielded a value of 79.14% flux at 6 hours. According to ICH guidance, the in-situ Lercanidipine HCl gel has turned up to be stable following a 6-month stability investigation. Thus, the subsequent thermoreversible in-situ gel was scouted to act as a potent nasal distributor with greater bioavailability and patient compliance for the nasal medication.
In-vitro Evaluation for Anticancer Activity of Extract of Asparagus racemosus
Authors: Jilakara Venkata Suresh , Velumani Arun Nivas , Panda Rudraksh Shovan , Lad Ganesh Sanjivan
Published: July 2024

The main purpose of this study is to look into how protective Asparagus racemosus leaves are in-vitro. Cancer is a major reason people die around the world. Many of the bad effects of the allopathic drugs that are now used to treat cancer have caused people to turn to plant medicine. Some of the solvents that were used in the sequential solvent extraction method were chloroform, ethanol and ethyl acetate. The chloroform extract had a higher percentage yield compared to the other extracts. Because it had anticancer activity in-vitro, the therapeutically active extract was picked to have anticancer activity in living things. The MTT test was used to see if the chloroform, ethyl acetate, and ethanol extracts could help fight cancer in HeLa cell types. The MTT assay showed that chloroform and ethyl acetate extracts had strong anticancer activity. These two extracts were chosen for study into how they work against cancer in living things
Moracin Flavonoids-based Virtual Screening Using Extended Reduced Graph Fingerprints Pharmacophore for COX-1 and PDES Targeting in Breast Cancer
Authors: Pathak CancerLeena , Thorat Mahesh , Gholap Pranjal , Bhise Manish
Published: July 2024

In order to create successful treatments for breast cancer, it is crucial to understand the binding interactions between flavonoids and phosphodiesterase (PDEs) and cyclooxygenase 1 (COX-1). This study uses computational methods to achieve just that. For the purpose of treating breast cancer, this study offers a thorough computational approach for finding possible COX-1 and PDEs inhibitors. Starting with the selection of bioactive compounds from the ChMBI database, the study makes use of a combination of pharmacophore-based and structural techniques. To perform molecular docking simulations with the indicated medicines, the PDB-REDO refined PDE4B2B and human COX-1 crystal structure were utilized. The binding affinities and interaction patterns of particular drugs with the COX-1 and PDEs are revealed by molecular docking simulations performed with the AutoDock program. The virtual screening results indicate that compounds with attractive interaction patterns, high binding affinities, and good structural compatibility could be good lead candidates. ADMET lab 2.0 tool for filtration was used to guarantee safety and effectiveness, offering information on the toxicity profile and pharmacokinetic characteristics of the chemical compound. Across numerous toxicity classes and endpoints, the chosen chemical, moracin C (C19H18O4), shows an anticipated inactivity and a largely good safety profile. Although immunotoxicity is anticipated, the overall low likelihood points to a comparatively modest risk. Moracin C has the potential to be developed into a medication, according to physicochemical and pharmacokinetic evaluations
Dengue in Wardha City: Addressing Social and Clinical Challenges
Authors: Ugemuge Pooja K , Khandalkar Gaurav G , Ingle Rahul G
Published: July 2024

Dengue fever, a major public health concern in tropical and subtropical areas, is a critical issue in Wardha city. The purpose of this study was to analyze Wardha inhabitants’ awareness, knowledge, and habits about dengue illness. The survey, which took place between November 2023 and April 2024, used a structured questionnaire and was delivered to a representative sample of 50 participants from various wards of the city. The questionnaire inquired about respondents’ knowledge of dengue symptoms, mechanisms of transmission, preventative measures, and attitudes toward local dengue control initiatives. Preliminary data show that, while many locals are aware of dengue and its symptoms, there are significant gaps in knowledge about effective preventative techniques, as well as widespread misunderstandings regarding transmission. The poll also preventative measures. Furthermore, the survey found diverse degrees of community engagement in dengue management operations, with many respondents advocating for more thorough and accessible teaching campaigns. These findings show the critical need for targeted educational initiatives and increased community engagement in Wardha city to improve dengue prevention and control. Public health experts and politicians are encouraged to use these findings to create and execute more effective, context-specific dengue control measures.
Unlocking Novel Therapeutic Avenues: Drug Repurposing and Virtual Screening for Breast Cancer Targeting the Estrogen Receptor
Authors: Jagtap Vaibhavkumar , Kakad Pooja , Suryawanshi Jayprakash , Patil Nitinchandra
Published: July 2024

This study explores the potential of drug repurposing for breast cancer therapy through virtual screening of Food and Drug Administration (FDA)-approved drugs against breast cancer-related targets. The estrogen receptor alpha ligand-binding domain, a key player in breast cancer progression, served as the primary target for virtual screening. Utilizing the DrugRep Virtual Screening Server and AutoDockVina, several compounds were identified with high binding affinities to the target protein. Notably, estradiol, benzhydrocodone, and ezetimibe emerged as top hits, showcasing diverse physicochemical properties and suggesting novel therapeutic possibilities. The structural fidelity of the estrogen receptor complex was validated through PDB-REDO refinement, enhancing the reliability of the binding interactions predicted. These findings underscore the potential of drug repurposing as a strategy for uncovering new therapeutic options in breast cancer treatment, warranting further biological validation and clinical exploration
An Innovative HPTLC-based Approach for the Quantification of Withaferin A in Combinations of Herbal Compounds
Authors: Surwade Kunal , Usman Md. Rageeb Md. , Vadnere Gautam , Raut Abhijit
Published: July 2024

Withaferin A in herbal mixed dose forms was quantified utilizing a new destructive reagent that is quick, accurate, and economical. Toluene, ethyl acetate, and formic acid were mixed in a mobile phase with a ratio of 5:4:2 v/v/v, and separation was carried out at a detection wavelength of 254 nm. In accordance with ICH guidelines, the procedure was verified. A range of 90 to 630 ng/band was determined for the calibration. The method was validated in three separate experiments with varying concentrations of the standard additive. The limit of detection (LoD) of 2.43 ng and the limit of quantitation (LoQ) of 7.54 ng were noted. During system adaptability testing, %RSD was under 2. Using the same approach, unchanged, on nine different formulations proved the method’s separation and quantification efficiency
Simultaneous Quantification of Dutasteride and Silodosin Using a Stability-Indicating RP-HPLC Approach
Authors: Pathade Parag , Harde Madhuri , Aher Bhaskar , Bairagi Vinod
Published: July 2024

An alternative stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) approach remained established to quantify silodosin (SLD) and dutasteride (DTS) in the mixture, validated per International Council of Harmonization (ICH) recommendations. A Shimadzu Model LC-2030 PLUS (IND) HPLC system, 231 nm detection wavelength, and 10 minutes run duration were used to create the method. A 75:25 mobile phase of methanol and water with 0.05% OPA was used at 1-mL/min. The concentrations of SLD (8–40 μg/mL) and DTS (0.5–2.5 μg/mL) were shown to be linearly related, with R2 values of 0.9998 for SLD and 0.9993 for DTS. Both of the drugs had accuracy and precision within 2% RSD. SLD and DTS mean recoveries were 97.5 to 102%. According to ICH criteria, degradation trials with acid, alkali, oxidizing agent, and heat assessed the approach’s stability. The established approach is fast, accurate, sensitive, and precise for routine SLD and DTS analysis from bulk as well as pharmaceutical dosage forms.
Fast and Sensitive Bioanalytical Method Development and Validation for Determination of Capmatinib in Human Plasma Using HPLC-MS/MS
Authors: Jabeen Nameera , Haque M Akiful
Published: July 2024

The term “anticancer drugs” evokes memories of a time when the development of medications in that category was still necessary. Capmatinib, for example, is a kinase inhibitor that targets the C-Met receptor tyrosine kinase in the treatment of non-small cell lung cancer, which involves tissue repair and organ regeneration.1 Thus, utilizing liquid chromatography-tandem mass spectroscopy (LC-MS/MS) in human plasma in accordance with United States Food and Drug Administration (USFDA) bioanalytical technique validation criteria, a quick, simple, specific, dependable, and sensitive approach was created using deucravacitinib as an internal standard. Capmatinib and the internal standard were separated using liquid-liquid extraction. The extracted sample run through a chromatographic system with an ACE-C18 column (4.6 × 100 mm, 5 μm); the mobile phase consisted of methanol and 2 mM ammonium formate in an 80:20 ratio at a flow rate of 1.00 mL/min. The system operates for three minutes in multiple reaction monitoring mode at the ABSCIEX API 4000 mass spectrometer using electron spray ionization. For capmatinib, the ion transitions are 413.10 to 382.10 and 426.30 to 358.20 for deucravacitinib, with a concentration range of 5.00 to 4000 ng/mL, the accuracy, precision, linearity, selectivity, and selectivity were validated and found to be within the acceptability limits.
Pharmacognostic and Preliminary Phytochemical Investigations of Borassus flabellifer Linn. Flowers
Authors: Mohite Madhuri Patil , Yadav Hemant KS , Raizaday Abhay
Published: July 2024

Borassus flabellifer Linn. commonly known as palmyra pam. The main purpose of the present work was to conduct systematic investigation of various extracts of B. flabellifer. Linn. flowers. The flowers of B. flabellifer Linn. belonging to family Areaceae were collected from Pune district. Authenticated at Botanical Survey of Pune. In this study B. flabellifer. Linn. flowers were evaluated for morphological characteristics, powdered microscopy, pharmacognostic investigation, preliminary phytochemical investigation as per World Health Organization (WHO) guidelines. The soxhlet apparatus was used for extraction, along with petroleum ether, ethyl acetate, chloroform and a hydro-alcoholic solvent. Flowers were also extracted by cold maceration. Morphologically flowers were showed a yellowish-brown color, sweet, pleasant taste. Powdered microscsopy was showed presence of fibres, vessels, crystals of calcium oxalate, starch grains. Pharmacognostic investigation shows foreign matter- Nil, total ash, water-soluble, acid-insoluble, sulphated ash was observed to be 3.5, 0.5, 0.5, 4.5%, respectively. LoD was found to be 4.6%. Petroleum ether, ethyl acetate, chloroform, ethanol and water-soluble extractive values were observed to be 0.2, 2.6, 0.4, 5.8 and 7.6%, respectively. Fluorescence analysis was also done. Extract contains carbohydrates, proteins, steroids, triterpenoids, glycosides, flavonoids, tannins and phenolic compounds. Present study could be helpful for the proper identification and further research work.
Simultaneous RP-HPLC Estimation, Validation and Stability Indicating Assay of Two-Component Tablet Formulation Containing Grazoprevir and Pibrentasvir
Authors: Srisailam Nakka , Vallakeerthi Narmada , Moorthy Revathy Sundara , Marapakala Kavitha
Published: July 2024

The determination of a two-component tablet formulation containing grazoprevir and pibrentasvir for method development and validation has been done using the reverse-phase high-performance liquid chromatography (RP-HPLC) method as per International Council of Harmonization (ICH) guidelines. Analytical grade acetonitrile and 0.01 N of potassium dihydrogen phosphate buffer were used as mobile phase while Kromasil C18 column was opted for separation. In order to elute the analyte a flow rate of 1-mL/min having 260 nm λ has been maintained. An RT of 2.909 and 2.358 minutes while linearity concentration range from 25 to 150 μg/mL and 10 to 60 μg/mL was obtained for grazoprevir and pibrentasvir, respectively, with 4.4 resolution. Both had a high correlation coefficient of 0.999. The limit of quantification was 0.24 and 0.09 μg/mL, while the detection limit at 0.72 and 0.27 μg/mL was noticed for grazoprevir and pibrentasvir, respectively. For grazoprevir and pibrentasvir, the regression equation was determined to be y = 16332x + 5313.1 and y = 17261x + 601.95, correspondingly
Formulation Development and Evaluation of Effervescent Granules of Rizatriptan Benzoate
Authors: SJ Jadhav , AB Gangurde , JA Jadhav
Published: July 2024

The present study describes the formulation and development of rizatriptan benzoate (RZB) effervescent granules and compares its dissolution rate against the conventional rizatriptan benzoate tablets. Earlier, placebo-effervescent granules were prepared to optimize the concentration of citric acid, NaHCO3 and tartaric acid and its effect on CO2 content and effervescence time. Effervescent granules were prepared by direct sifting and blending of ingredients. Rizatriptan effervescent granules were prepared by the addition of RZB in the above-optimized placebo granule formula and evaluated for various physicochemical parameters. Later, the effervescent RZB granules were compared with the marketed RZB tablets for the dissolution rate. It was observed that RZB effervescent granules have the fastest dissolution rate compared with marketed RZB tablets. The optimized RZB effervescent granule formulation is quite superior to that of RZB conventional tablets to relieve the patients from migraine and the possible associated hyperacidity it.
The Importance of Pharmacovigilance in the Polyherbal Sector: Challenges and Future Potential
Authors: Gupta Divyanshi , Sudan Puneet , Imra Mohd , Wal Ankita
Published: July 2024

Pharmacovigilance is crucial in ensuring the safety and potency of polyherbal products, as many have not been extensively evaluated for their pharmacology and toxicity. With the increasing popularity of polyherbal medications, there has been a rise in complaints of probable toxicity and adverse outcomes. This study investigates the importance of pharmacovigilance in the polyherbal sector and examines its challenges and future potential. Data was collected from various databases from 2000-2024. Pharmacovigilance has become a key domain in medicine and public health, with a significant portion of India’s population relying on Indian medical systems. The study highlights the importance of implementing pharmacovigilance procedures for polyherbal medications, promoting rational use and appropriate treatment methods. Polyherbal drugs may have negative interactions with other medications, foods, biologicals, and compounds, leading to decreased therapeutic efficacy, undesirable side effects, and severe complications. Systematic pharmacovigilance is necessary to collect accurate data and develop guidelines for safe and effective use.
Pharmacological and Phytochemical Potential of Andropogon muricatus: A Review
Authors: Khobragade Samiksha , Ali Tayyaba S , Kediya Ankit , Gandhare Bhushan
Published: July 2024

Many medicinal plants have been utilized as medications since ages ago. Chemical components extracted from such plants have been used historically in traditional Unani medicine, the Siddha and other medical systems for a variety of ailments. Among them is Andropogon muricatus, a member of the family Poaceae, better known by its common name, vetiver. It contains a variety of organic components with significant therapeutic benefits for a range of illnesses, including mosquito repellent, anxiolytic, acaricidal, hypoglycaemic, antidepressant, antidiuretic, sedative, antifungal, and nervine effects. This plant’s roots serve as cooling. When it comes to oil extraction—which is used in food and beverages, spas, home appliances, and medicine—root systems are the most valuable parts of the plant. It is indigenous in Bangladesh, China, Japan, the Bonin Islands, Nepal, the Philippines, and Sri Lanka in the Asian continent. Australia and Spain were first exposed to it in Europe and Oceania. Substances of a chemical nature that have been extracted from the plant’s overall various sections have previously been the subject of numerous pharmacologic and medicinal studies.
Multidimensional Perspectives on Psoriasis Treatment: Current State and Future Directions
Authors: Thalathoti Vandana Raj , Thakur Santh Rani
Published: July 2024

Millions of people are afflicted with psoriasis, which is a chronic inflammatory skin disease throughout the world. Despite modern treatments for psoriasis remain unmet needs and challenges in achieving optimal results. This review article aims to present a multidimensional view of the research and therapy for psoriasis, which encompasses not only the cellular and molecular mechanisms but also the network pharmacology of traditional Chinese medicine, novel therapeutic targets and strategies as well as autophagy plays a role on psoriasis pathogenesis or treatment. At the same time discussion on bimekizumab, once was declared an initial proof of concept biological agent that acts by both inhibiting interleukin-17 A and F (two cytokines slightly different) in psoriasis.
Green Synthesis of Nanoparticles for Combatting Antibiotic-Resistant Bacterial Infections: A Comprehensive Review
Authors: Sheikh Mujibullah , Mude Gaurav
Published: July 2024

Innovative strategies are required to address this worldwide dilemma since the rise of antibiotic-resistant bacterial illnesses presents a serious danger to public health. The environmentally benign and sustainable process of green synthesis of nanoparticles has drawn interest as a means of creating antibacterial nanomaterials. This thorough analysis looks at the most recent developments in creating nanoparticles that fight bacterial illnesses resistant to antibiotics by using green production processes. In order to create nanoparticles with increased antimicrobial activity, the study highlights the efficacy of many green synthesis techniques, including the use of plant extracts, microbial sources, and other natural materials. The study also looks at these green-synthesized nanoparticles’ methods of action against bacteria that are resistant to antibiotics and their therapeutic uses. Through the synthesis of nanoparticles utilizing environmentally friendly methods, this study seeks to combat antibiotic resistance by offering a comprehensive overview of the existing research landscape and encouraging the development of innovative solutions.
Formulation, Development and Characterization of Oral Jelly to Improve Therapeutic Effectiveness
Authors: Komal Khade , Nilesh Kulkarni , Vaibhav Bhamare , Rakesh Amrutkar
Published: July 2024

The oral route is the most convenient and has great effectiveness in taking new chemical entities; hence it has improved patient acceptance. However, the main limitations associated with such formulations involve unpleasant or bitter taste as well as problems related to swallowing and reduced bioavailability of chemical entities. When it comes to children, the main limitation is they cannot safely swallow the medications in the form of tablets and capsules. But kids, even those without teeth, can swallow the jelly easily. As in the development of new dosage forms for every child, taste, color, smell, texture, and appearance are important factors in improving patient compliance. Children refuse to tolerate the same medication again, which becomes a big problem for parents trying to take the medication. An effective way to solve such types of problems involves the production of children’s friendly dosage formulations with attractive and eye catching taste, smell, color as well as texture. The most identical characteristic of oral jelly as dosage form is that it are easy to chew and dissolves rapidly in saliva so no water is required. Moreover, the good texture and appearance make it easy to attract patients as well as to improve patient compliance. Above all, it provides a soft and beautiful texture, causing no discomfort to patients.
A Case Series of Monoclonal Immunoglobulin-Depositing Proliferative Glomerulonephritis
Authors: Venkatesh Leelavathi , Shetty Bhushan Chandrahasa
Published: July 2024

Proliferative glomerulonephritis with monoclonal immunoglobulin deposition disease (PGNMIDD) is a rare entity. We evaluated the clinicopathological features of PGNMIDD and the effectiveness of different treatment regimens in 13 cases diagnosed using kidney biopsy. Most had chronic kidney disease followed by acute nephritic syndrome, rapidly progressive glomerulonephritis, and nephrotic syndrome. Membranoproliferative glomerulonephritis was the most common pattern of renal injury. Three patients had abnormal bone marrow studies. Different treatment regimens were deployed; >60% had partial remission at the end of six months and 30.7% progressed to end stage renal disease.
Percutaneous unilateral vertebro-stentoplasty for painful osteoporotic compression fracture of lumbar vertebra
Authors: Karki Mohan , Rajpal Girish , Bundela Yashpal Singh , Vaish Manish
Published: July 2024

Osteoporosis is one of the common diseases in the elderly population which can cause compression fracture of the vertebral body due to loss of microarchitecture, loss of density, and deterioration of bone tissue leading to chronic pain, kyphotic deformity of the vertebra, and neurological complications. We report the case of a 68-year-old female, who presented to us with severe midback region pain for one year, which was increased over two months along with numbness in the right leg and difficulty in walking. The radiological investigation and magnetic resonance imaging spine revealed chronic compression fracture with the reduction in height of the L1 vertebra, which is seen with anterior wedging. The patient underwent vertebro-stentoplasty (VS) with bone cement, polymethyl-methacrylate. The patient was discharged well on third day of surgery. The VS for compression fracture of the lumbar vertebra demonstrated better results with improvement in pain and radiological finding in our patient.
PENGARUH KOMPETENSI SDM DAN PENERAPAN SIA TERHADAP KUALITAS LAPORAN KEUANGAN
Authors: Emkhad Arif  , Zelnita Aulia Fitri
Published: July 2024

The purpose of this study was to determine the effect of human resource competence and the application of accounting information systems on the quality of financial reports for small and medium enterprises. This research was conducted in Siak and Kandis Districts. The types of data used in this study are primary data and secondary data. Primary data is data obtained by quesioners with business employees or business owners who handle the preparation of financial statements. Secondary data is data obtained from documents and reports related to research. Data collection techniques used are interviews, questionnaires and documentation. While the data analysis technique is multiple linear regression analysis. The results showed that the variable of human resource competence had a significant effect on the quality of the financial statement of small and medium enterprises, while the application of an accounting information system had a significant effect on the quality of financial reports and the competence of human resources and the application of an accounting information system had a significant on the quality of business financial statement small and medium.
PENGARUH TINGKAT PENDIDIKAN, PENGETAHUAN AKUNTANSI, UMUR USAHA DAN SKALA USAHA PELAKU USAHA MIKRO KECIL DAN MENENGAH (UMKM) TERHADAP PENGGUNAAN INFORMASI AKUNTANSI DI KECAMATAN SENAPELAN
Authors: Karin  , Abrar
Published: July 2024

This study aims to determine the effect of education level, accounting knowledge, business age and business scale of micro, small and medium enterprises (MSMEs) on the use of accounting information in Senapelan District. The method used in this research is simple random sampling. The type of data used is primary data. Data collection carried out in this study is the distribution of questionnaires. The sample of this study consisted of 74 businesses. Data processing was carried out using SPSS version 21 program tools. The research model used by the researcher was multiple linear regression analysis. Based on the results of the tests carried out, it was concluded that the level of education, accounting knowledge, business age and business scale had a significant effect on the use of accounting information on MSMEs in Senapelan District.
ANALISIS PENGARUH KINERJA LINGKUNGAN DAN BIAYA LINGKUNGAN TERHADAP PROFITABILITAS DENGAN UKURAN PERUSAHAAN SEBAGAI VARIABEL MODERATING (PADA PERUSAHAAN SEKTOR INDUSTRI DAN KIMIA YANG TERDAFTAR DIBURSA EFEK INDONESIA PERIODE 2017-2020)
Authors: Hana Fahira  , Yusrawati
Published: July 2024

This study aims to determine and analyze the effect of environmental performance and environmental costs on profitability with firm size as a moderating variable in industrial and chemical sector companies listed on the Indonesia Stock Exchange for the 2017-2020 period. This research includes quantitative research using secondary data. The population in this study is the industrial and chemical sector companies listed on the Stock Exchange, with sampling using purposive sampling technique, which aims to obtain samples that match the research criteria. The sample used was 11 companies in the period 2017 to 2020. The data analysis techniques used were multiple regression analysis and moderated regression analysis (MRA). Based on the results of the tests that have been carried out, it shows that partially environmental performance has no effect on profitability, while environmental costs have an effect on profitability. Simultaneously, environmental performance variables, environmental costs, and firm size affect profitability. The moderating analysis of the firm size variable cannot moderate the relationship between environmental performance and profitability and the firm size variable cannot moderate the relationship between environmental costs and profitability.
ANALISIS PENGARUH UKURAN PERUSAHAAN, FINANCIAL DISTRESS DAN OPINI AUDIT TERHADAP KETEPATAN WAKTU PELAPORAN KEUANGAN PADA PERUSAHAAN INDUSTRI BARANG KONSUMSI YANG TERDAFTAR DI BURSA EFEK INDONESIA (BEI) PERIODE 2018-2020
Authors: Septa Risanty Anggi , Alfurkaniati  , Burhanuddin
Published: July 2024

This study aims to analyze the effect of Company Size, Financial Distress, and Audit Opinion on the Timeliness of Financial Reporting. The population used in this study was a Consumer Goods Industry company listed on the Indonesia Stock Exchange for the period 2018 to 2020 which amounted to 64 companies. Based on the sampling method using the Purposive Sampling method, a sample of 47 companies was obtained for 3 (three) years of observation from 2018 to 2020 with 141 observations (observations). The data analysis techniques used in this study are descriptive statistical analysis, logistic regression analysis and hypothesis testing using the SPSS 25 program. The results of this study show that Company Size and Financial Distress do not have a significant effect on the timeliness of financial reporting, while Audit Opinion has a significant effect on the Timeliness of Financial Reporting and the variables of Company Size, Financial Distress and Udit Opinion have a simultaneous effect on the timeliness of financial reporting.
DETERMINAN KINERJA PENGELOLAAN KEUANGAN DESA DI KABUPATEN KAMPAR
Authors: Burhanuddin  , Alfurkaniati  , Rahmat Ismael yunef
Published: July 2024

This research was conducted in villages in Gunung Sahilan District, Kampar Regency, with the aim of research to determine the Effect of Transparency, Accountability, Role of Village Apparatus, Community Participation and Effectiveness of Village Financial Management Performance, (Empirical Study on Villages in Gunung Sahilan District, Kampar Regency). This research is part of a quantitative research that is correlational. The data used are primary data with data collection techniques used are questionnaires and data analysis techniques using multiple regression methods. The results obtained indicate that partially and simultaneously the variables of Transparency, Accountability, Role of Village Apparatus, Community Participation and Effectiveness have a positive and significant effect on Village Financia lmanajement Performent.
ANALISIS VALUE FOR MONEY PADA KINERJA KEUANGAN DINAS LINGKUNGAN HIDUP KABUPATEN ROKAN HULU
Authors: Putra M. Fauzan , Nursida Nina
Published: July 2024

This study aims to analyze the performance accountability of the Rokan Hulu District Environmental Office using the principle of value for money. The type of research used is qualitative research. The collection technique obtained was in the form of Performance Reports of Government Agencies at the Rokan Hulu Regency Environmental Service for 2020 and 2021. The results of this study indicate that in 2020 economical calculations with a ratio value of 72% are categorized as economical, efficient calculations with a ratio value of 131% are categorized as efficient, and effectiveness calculations with a ratio value of 93% are categorized as ineffective. In 2021 economic calculations with a ratio value of 93.32% are categorized as economical, efficient calculations with a ratio value of 101.77% are categorized as efficient, and effectiveness calculations with a ratio value of 59% are categorized as ineffective. Thus the performance of the Rokan Hulu Regency Environmental Service in 2020 and 2021 in terms of economic elements, efficiency and effectiveness has not fulfilled the principle of value for money.