bluuebunny/crossref_metadata_2025 huggingface dataset:
Age/autoimmunity-associated B cells in inflammatory arthritis: An emerging therapeutic target
Authors: Li Zhen-yu , Cai Ming-Long , Qin Yi , Chen Zhu
Published: January 2023

Age/autoimmunity-associated B cells (ABCs) are a novel B cell subpopulation with a unique transcriptional signature and cell surface phenotype. They are not sensitive to BCR but rely on TLR7 or TLR9 in the context of T cell-derived cytokines for the differentiation. It has been established that aberrant expansion of ABCs is linked to the pathogenesis of systemic autoimmune diseases such as systemic lupus erythematosus. Recently, we and other groups have shown that increased ABCs is associated with rheumatoid arthritis (RA) disease activity and have demonstrated their pathogenic role in RA, indicating that targeting specific B cell subsets is a promising strategy for the treatment of inflammatory arthritis. In this review, we summarize the current knowledge of ABCs, focusing on their emerging role in the pathogenesis of inflammatory arthritis. A deep understanding of the biology of ABCs in the context of inflammatory settings in vivo will ultimately contribute to the development of novel targeted therapies for the treatment of inflammatory arthritis.
Macrofauna-sized foraminifera in epibenthic sledge samples from five areas in the eastern Clarion-Clipperton Zone (equatorial Pacific)
Authors: Gooday Andrew J. , Wawrzyniak-Wydrowska Brygida
Published: January 2023

Benthic foraminifera cannot be sampled adequately using a single device. Smaller taxa are best collected using multicorers, the larger with box corers, but towed devices (dredges, trawls and epibenthic sledges) also retain many larger species. Here, we describe macrofaunal (>300 µm) foraminiferal assemblages obtained using an epibenthic sledge (EBS) in the Clarion-Clipperton Zone (eastern equatorial Pacific), a region hosting seafloor deposits of polymetallic nodules. Twelve EBS samples were collected in four areas licenced for exploration by the International Seabed Authority (ISA) to German, IOM, Belgium and French contractors, and to APEI-3, one of the protected Areas of Special Scientific Interest designated by the ISA. We recognised 280 morphospecies among 1954 specimens, with between 74 (IOM) and 121 (Belgium) in particular areas. Most (92.7%) were single-chambered monothalamids, of which 75 species (26.8%) belonged to the Komokioidea (‘komoki’), 47 (16.8%) to branched and unbranched tubes, 33 (11.8%) to chain-like and 32 (11.4%) to various ‘komoki-like’ forms. Fragments of megafaunal xenophyophores represented 21 species (7.50%), including Spiculammina delicata, previously reported only from the Russian area. Rarefaction curves and sample coverage completeness curves suggest that only a fraction of the macrofaunal foraminiferal diversity had been sampled. The occurrence of 71.8% of species in 1-2 of the 12 samples and 84.9% in 1-3 of the samples was a likely result of substantial undersampling. Dissimilarity in species composition between areas was very high: 64.2% (German vs IOM area) to 86.9% (German area vs APEI-3). Similarity within a single area was quite low: 29.1% (German) to 45.1% (IOM). In multidimensional scaling (MDS) plots, the APEI-3 area was clearly distinct in terms of faunal composition from all other areas, the French area somewhat separated from the German, IOM and Belgium areas, with the German and IOM samples being the most similar. These patterns may reflect the geographical separation of the French and APEI-3 areas and their location in deeper, more oligotrophic waters. Our study demonstrates that EBS samples from the eastern CCZ are a rich source of novel foraminiferal taxa, particularly light, easily resuspended komoki, providing a valuable perspective on foraminiferal biodiversity.
The path of transboundary marine plastic waste management in China, Japan, and South Korea from the perspective of the blue economy
Authors: Hao Huijuan , Jiang Chenfan
Published: January 2023

Marine plastic waste is one of the most difficult global ocean governance issues at present, and is also the focus of marine waste governance in the Yellow Sea and East China Sea region surrounded by China, Japan, and Republic of Korea (ROK). China, Japan, and Republic of Korea (ROK)are now aware of the importance of this issue to their surrounding waters and their country’s development. However,the poor implementation of marine governance gives rise to the unsatisfactory effect of marine plastic waste governance in the sea area. Based on the concept of blue economy and the data of plastic pollution in the Yellow Sea and East China Sea, this paper discusses the feasibility of establishing binding legal norms and policies to promote the progress of marine plastic waste treatment in China, Japan and Republic of Korea. By using research methods of text analysis and status survey, this paper analyzes the differences and common demands of China, Japan and Republic of Korea for marine plastic pollution control. It is concluded that three countries have common demands in the management of the whole life cycle of marine plastic pollution, the establishment of flexible legal instruments, and the participation of stakeholders. Finally, through the case experience of the blue cycle model of marine plastic waste in Taizhou, Zhejiang Province, China, this paper proposes that this model can also be used in the management of marine plastic waste in China, Japan and Republic of Korea. Three countries can gradually promote the cooperation of marine plastic waste laws and regulations by starting with the treatment of\ fishery plastic waste, and innovate the blue cycle model, so as to finally promote the conclusion of the regional marine waste treatment agreement.
Seizure burden and neuropsychological outcomes of new-onset refractory status epilepticus: Systematic review
Authors: Taraschenko Olga , Pavuluri Spriha , Schmidt Cynthia M. , Pulluru Yashwanth Reddy , Gupta Navnika
Published: January 2023

Background

Long-term sequelae of the new onset refractory status epilepticus (NORSE) include the development of epilepsy, cognitive deficits, and behavioral disturbances. The prevalence of these complications has been previously highlighted in case reports and case series: however, their full scope has not been comprehensively assessed.

Methods

We conducted a systematic review of the literature (PROSPERO ID CRD42022361142) regarding neurological and functional outcomes of NORSE at 30 days or longer following discharge from the hospital. A systematic review protocol was developed using guidance from the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA).

Results

Of the 1,602 records for unique publications, 33 reports on adults and 52 reports on children met our inclusion criteria. They contained the description of 280 adults and 587 children of whom only 75.7 and 85% of patients, respectively had data on long-term follow-up. The mean age of adult and pediatric patients was 34.3 and 7.9 years, respectively; and the longest duration of follow up were 11 and 20 years, respectively. Seizure outcomes received major attention and were highlighted for 93.4 and 96.6% of the adult and pediatric NORSE patients, respectively. Seizures remained medically refractory in 41.1% of adults and 57.7% of children, while seizure freedom was achieved in only 26 and 23.3% of these patients, respectively. The long-term cognitive outcome data was provided for just 10.4% of the adult patients. In contrast, cognitive health data were supplied for 68.9% of the described children of whom 31.9% were moderately or severely disabled. Long-term functional outcomes assessed with various standardized scales were reported in 62.2 and 25.5% of the adults and children, respectively with majority of patients not being able to return to a pre-morbid level of functioning. New onset psychiatric disorders were reported in 3.3% of adults and 11.2% of children recovering from NORSE.

Conclusion

These findings concur with previous observations that the majority of adult and pediatric patients continue to experience recurrent seizures and suffer from refractory epilepsy. Moderate to severe cognitive disability, loss of functional independence, and psychiatric disorders represent a hallmark of chronic NORSE signifying the major public health importance of this disorder.
Dosage consideration for transcranial direct current stimulation in post-stroke dysphagia: A systematic review and network meta-analysis
Authors: Xie Jianwei , Zhou Chiteng , Ngaruwenayo Gilbert , Wu Minghui , Jiang Xiaoyu , Li Xiaohan
Published: January 2023

Objective

This systematic review and network meta-analysis sought to determine the efficacy of different intensities of transcranial direct current stimulation (tDCS) in patients with dysphagia after stroke to improve swallowing function.

Methods

Randomized-controlled trials (RCTs) of tDCS in post-stroke dysphagia were searched from Pubmed, EMBASE, Cochrane Library, Web of Science, China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Service System (SinoMed), Wanfang database, and Chinese Scientific Journals Database (VIP) from databases' inception to June 22, 2022. Article screening, data extraction, and article quality evaluation were completed by 2 independent researchers. Network meta-analysis was performed using Stata.

Results

A final total of 20 studies involving 838 stroke patients were included. The included control interventions were sham tDCS and conventional therapy (CT). Network meta-analysis showed that 20 min of 1.2, 1.4, 1.5, 1.6, and 2 mA anodal tDCS and 30 min of 2 mA anodal tDCS significantly improved post-stroke dysphagia compared with CT (all P < 0.05). In addition, 20 min of 1, 1.4, 1.6, and 2 mA anodal tDCS also significantly improved post-stroke dysphagia compared with sham tDCS (all P < 0.05). Our results demonstrated that 20 min of stimulation at 1.4 mA was the optimal parameters for anodal tDCS and exhibited superior efficacy to CT [SMD = 1.08, 95% CI (0.46, 1.69)] and sham tDCS [SMD = 1.45, 95% CI (0.54, 2.36)].

Conclusion

Different durations and intensities of anodal tDCS are effective in improving post-stroke dysphagia. However, 20 min of tDCS at 1.4 mA may be the optimal regimen.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42022342506.
The contribution of coherence field theory to a model of consciousness: electric currents, EM fields, and EM radiation in the brain
Authors: Bond Eric
Published: January 2023

A paradigm in neuroscience is developing which views resonance as the phenomenon responsible for consciousness. Much progress is being made in the investigation of how resonance as oscillating flows within the brain’s electric field might result in production of mind from matter. But it’s mostly unknown how vibrations among features of matter such as nanoscale atomic structures and photonic waves may participate in forming the basic substance of first-person consciousness, meaning percepts such as colors, textures, sounds, thoughts, feelings et cetera. Initial evidence at the leading edge of quantum biology suggests that light and atoms combine to form synchronously resonating structures of contiguous energy which I have termed coherence fields. My hypothesis is that coherence fields as atomic nodes within expanses of integrating photonic waves are the fundamental unit of first-person percepts insofar as they arise from electromagnetic matter. A concept of quantum coherence is formulated based on a new phenomenology of matter’s nanoscale properties, and this is shown to tie what we have thus far discovered of neural anatomy into a comprehensive model of how electrical impulses travel through neurons as electron currents driven by coherence at the quantum scale. Transmembrane electric fields generated by ionic currents, synaptic phase regulation, and perhaps further mechanisms have been hypothesized as responsible for local field potentials (LFP) oscillations. Some insights into how emergent, macroscopic waves in the brain’s electric field may reciprocally impact LFP propagation to control arousal, attention, and volition are briefly discussed. Activation of neural tissue is closely linked to temperature variation, and it is hypothesized that this is not merely a waste byproduct but constitutes a signature of coherence field modulation, with photonic waves of a primarily infrared spectral range functioning as an interstitial medium of the basic percept field. A variety of possible routes to coherence field modulation are outlined that derive from the mechanisms of electric currents, EM fields, EM radiation, and entanglement. If future experimental designs continue to validate coherence field theory, this could set science on course to resolve the mind/body problem.
Enhancement of adenosine A2A signaling improves dopamine D2 receptor antagonist-induced dyskinesia via β-arrestin signaling
Authors: Nagaoka Koki , Asaoka Nozomi , Nagayasu Kazuki , Shirakawa Hisashi , Kaneko Shuji
Published: January 2023

Repeated administration of dopamine D2 receptor (D2R) antagonists, which is the treatment for psychosis, often causes tardive dyskinesia (TD). Despite notable clinical demand, effective treatment for TD has not been established yet. The neural mechanism involving the hyperinhibition of indirect pathway medium spiny neurons (iMSNs) in the striatum is considered one of the main causes of TD. In this study, we focused on adenosine A2A receptors (A2ARs) expressed in iMSNs and investigated whether pharmacological activation of A2ARs improves dyskinetic symptoms in a TD mouse model. A 21-day treatment with haloperidol increased the number of vacuous chewing movements (VCMs) and decreased the number of c-Fos+/ppENK+ iMSNs in the dorsal striatum. Haloperidol-induced VCMs were reduced by acute intraperitoneal administration of an A2AR agonist, CGS 21680A. Consistently, haloperidol-induced VCMs and decrease in the number of c-Fos+/ppENK+ iMSNs were also mitigated by intrastriatal injection of CGS 21680A. The effects of intrastriatal CGS 21680A were not observed when it was concomitantly administered with a β-arrestin inhibitor, barbadin. Finally, intrastriatal injection of an arrestin-biased D2R agonist, UNC9994, also inhibited haloperidol-induced VCMs. These results suggest that A2AR agonists mitigate TD symptoms by activating striatal iMSNs via β-arrestin signaling.
Transcranial near-infrared laser improves postoperative neurocognitive disorder in aged mice via SIRT3/AMPK/Nrf2 pathway
Authors: Zhong Junying , Zhao Le , Wu Wensi , Chen Jiawei , Yuan Shangyan , Zhang Xiaojun , Wang Zhi
Published: January 2023

Background

Postoperative neurocognitive disorder (PND) is a common central nervous system (CNS) complication that might increase the morbidity and mortality of elderly patients after anesthesia/surgery. Neuroinflammation, oxidative stress, and synaptic dysfunction are closely related to cognitive dysfunction, an important clinical feature of PND. Transcranial near-infrared laser (TNIL) is regarded as an effective treatment for cognitive-related diseases by improving mitochondrial function and alleviating neuroinflammation and oxidative stress damage.

Materials and methods

Aged male C57BL/6 mice underwent a carotid artery exposure procedure under isoflurane anesthesia. We treated PND-aged mice for three consecutive days (4 h post-operation, 1-laser) with 810 nm continuous wave (CW) laser 18 J/cm2 at 120 mW/cm2. The post-treatment evaluation included behavioral tests, RTq-PCR, immunofluorescence, and Western blot.

Results

The results demonstrated that TNIL improved PND and the levels of synaptic function-associated proteins such as post-synaptic density protein 95 (PSD95), synaptophysin (SYP), and brain-derived neurotrophic factor (BDNF). Besides, neuroinflammatory cytokine levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β as well as microglia activation and oxidative stress damage were attenuated after TNIL treatment in aged mice with PND. Further investigation suggested that TNIL relieved oxidative stress response by activating the SIRT3/AMPK/Nrf2 pathway.

Conclusion

Transcranial near-infrared laser improved cognitive impairment in aged mice with PND, which may be a promising therapeutic for PND.
A genome-wide association study of a rage-related misophonia symptom and the genetic link with audiological traits, psychiatric disorders, and personality
Authors: Smit Dirk J. A. , Bakker Melissa , Abdellaoui Abdel , Hoetink Alexander E. , Vulink Nienke , Denys Damiaan
Published: January 2023

Introduction

People with misophonia experience strong negative emotional responses to sounds and associated stimuli—mostly human produced—to an extent that it may cause impairment in social functioning. The exact nature of the disorder remains a matter of ongoing research and debate. Here, we investigated the genetic etiology of misophonia to understand contributing genetic factors and shed light on individual differences in characteristics that are related to the disorder.

Methods

For misophonia, we used an unpublished genome-wide association study (GWAS) from genetic service provider 23andMe, Inc., on a self-report item probing a single common misophonic symptom: the occurrence of rage when others produce eating sounds. First, we used gene-based and functional annotation analyses to explore neurobiological determinants of the rage-related misophonia symptom. Next, we calculated genetic correlations (rG) of this rage-related misophonia symptom GWAS with a wide range of traits and disorders from audiology (tinnitus, hearing performance, and hearing trauma), psychiatry, neurology, and personality traits.

Results

The rage-related misophonia symptom was significantly correlated with tinnitus, major depression disorder (MDD), post-traumatic stress disorder (PTSD), and generalized anxiety disorder (GAD; 0.12 <rG< 0.22). Stronger genetic correlations (0.21 <rG< 0.42) were observed for two clusters of personality traits: a guilt/neuroticism and an irritability/sensitivity cluster. Our results showed no genetic correlation with attention deficit and hyperactivity disorder, obsessive-compulsive disorder, and psychotic disorders. A negative correlation with autism spectrum disorder (ASD) was found, which may be surprising given the previously reported comorbidities and the sensory sensitivity reported in ASD. Clustering algorithms showed that rage-related misophonia consistently clustered with MDD, generalized anxiety, PTSD, and related personality traits.

Discussion

We conclude that—based on the genetics of a common misophonia symptom—misophonia most strongly clusters with psychiatric disorders and a personality profile consistent with anxiety and PTSD.
Microwave-assisted extraction of cellulose nanocrystals from almond (Prunus amygdalus) shell waste
Authors: Valdés Arantzazu , Mondragon Gurutz , Garrigós María Carmen , Eceiza Arantxa , Jiménez Alfonso
Published: January 2023

Almond (Prunus amygdalus) is one of the most common tree nuts on a worldwide basis. This nut is highly regarded in the food and cosmetic industries. However, for all these applications, almonds are used without their shell protection, which is industrially removed contributing approximately 35-75% of the total fruit weight. This residue is normally incinerated or dumped, causing several environmental problems. In this study, a novel cellulose nanocrystal (CNCs) extraction procedure from almond shell (AS) waste by using microwave-assisted extraction was developed and compared with the conventional approach. A three-factor, three-level Box–Behnken design with five central points was used to evaluate the influence of extraction temperature, irradiation time, and NaOH concentration during the alkalization stage in crystallinity index (CI) values. A similar CI value (55.9 ± 0.7%) was obtained for the MAE process, comprising only three stages, compared with the conventional optimized procedure (55.5 ± 1.0%) with five stages. As a result, a greener and more environmentally friendly CNC extraction protocol was developed with a reduction in time, solvent, and energy consumption. Fourier transform infrared (FTIR) spectra, X-ray diffractogram (XRD), atomic force microscopy (AFM), and scanning electron microscopy (SEM) images, and thermal stability studies of samples confirmed the removal of non-cellulosic components after the chemical treatments. TEM images revealed a spherical shape of CNCs with an average size of 21 ± 6 nm, showing great potential to be used in food packaging, biological, medical, and photoelectric materials. This study successfully applied MAE for the extraction of spherical-shaped CNCs from AS with several advantages compared with the conventional procedure, reducing costs for industry.
Good nutrition across the lifespan is foundational for healthy aging and sustainable development
Authors: Rodríguez-Mañas Leocadio , Murray Robert , Glencorse Carole , Sulo Suela
Published: January 2023

Ensuring healthy lives and promoting wellbeing across the age spectrum are essential to sustainable development. Nutrition is at the heart of the World Health Organization (WHO) Sustainable Development Goals, particularly for Sustainable Development Goal 2/Subgoal 2, which is toEnd all forms of malnutrition by 2030. This subgoal addresses people of all ages, including targeted groups like young children and older adults. In recent decades, there have been marked advances in the tools and methods used to screen for risk of malnutrition and to conduct nutritional assessments. There have also been innovations in nutritional interventions and outcome measures related to malnutrition. What has been less common is research on how nutritional interventions can impact healthy aging. OurPerspectivearticle thus takes a life-course approach to consider what is needed to address risk of malnutrition and why, and to examine how good nutrition across the lifespan can contribute to healthy aging. We discuss broad-ranging yet interdependent ways to improve nutritional status worldwide—development of nutritional programs and policies, incorporation of the best nutrition-care tools and methods into practice, provision of professional training for quality nutritional care, and monitoring health and economic benefits of such changes. Taken together, ourPerspectiveaims to (i) identify current challenges to meeting these ideals of nutritional care, and to (ii) discover enabling strategies for the improvement of nutrition care across the lifespan. In harmony with the WHO goal of sustainable development, we underscore roles of nutrition to foster healthy human development and healthy aging worldwide.
m7G-related gene NUDT4 as a novel biomarker promoting cancer cell proliferation in lung adenocarcinoma
Authors: Liu Yafei , Jiang Bin , Lin Chunjie , Zhu Wanyinhui , Chen Dingrui , Sheng Yinuo , Lou Zhiling , Ji Zhiheng , Wu Chuanqiang , Wu Ming
Published: January 2023

Background

Lung cancer is the leading cause of mortality in cancer patients. N7-methylguanosine (m7G) modification as a translational regulation pattern has been reported to participate in multiple types of cancer progression, but little is known in lung cancer. This study attempts to explore the role of m7G-related proteins in genetic and epigenetic variations in lung adenocarcinoma, and its relationship with clinical prognosis, immune infiltration, and immunotherapy.

Methods

Sequencing data were obtained from the Genomic Data Commons (GDC) Data Portal and Gene Expression Omnibus (GEO) databases. Consensus clustering was utilized to distinguish m7G clusters, and responses to immunotherapy were also evaluated. Moreover, univariate and multivariate Cox and Least absolute shrinkage and selection operator LASSO Cox regression analyses were used to screen independent prognostic factors and generated risk scores for constructing a survival prediction model. Multiple cell types such as epithelial cells and immune cells were identified to verify the bulk RNA results. Short hairpin RNA (shRNA) Tet-on plasmids, Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas9 for knockout plasmids, and nucleoside diphosphate linked to moiety X-type motif 4 (NUDT4) overexpression plasmids were constructed to inhibit or promote tumor cell NUDT4 expression, then RT-qPCR, Cell Counting Kit-8 CCK8 proliferation assay, and Transwell assay were used to observe tumor cell biological functions.

Results

Fifteen m7G-related genes were highly expressed in tumor samples, and 12 genes were associated with poor prognosis. m7G cluster-B had lower immune infiltration level, worse survival, and samples that predicted poor responses to immunotherapy. The multivariate Cox model showed that NUDT4 and WDR4 (WD repeat domain 4) were independent risk factors. Single-cell m7G gene set variation analysis (GSVA) scores also had a negative correlation tendency with immune infiltration level and T-cell Programmed Death-1 PD-1 expression, but the statistics were not significant. Knocking down and knocking out the NUDT4 expression significantly inhibited cell proliferation capability in A549 and H1299 cells. In contrast, overexpressing NUDT4 promoted tumor cell proliferation. However, there was no difference in migration capability in the knockdown, knockout, or overexpression groups.

Conclusions

Our study revealed that m7G modification-related proteins are closely related to the tumor microenvironment, immune cell infiltration, responses to immunotherapy, and patients’ prognosis in lung adenocarcinoma and could be useful biomarkers for the identification of patients who could benefit from immunotherapy. The m7G modification protein NUDT4 may be a novel biomarker in promoting the progression of lung cancer.
Efficacy and safety of salvage radiotherapy combined with endocrine therapy in patients with biochemical recurrence after radical prostatectomy: A systematic review and meta-analysis of randomized controlled trials
Authors: Liang Zhanpeng , Lin Sihong , Lai Huiqin , Li Luzhen , Wu Jiaming , Zhang Huatang , Fang Cantu
Published: January 2023

Background

The addition of endocrine therapy to salvage radiotherapy (SRT) is expected to further improve the prognosis of patients with biochemical recurrence of prostate cancer after radical prostatectomy (RP). The quantitative synthesis of clinical outcomes of SRT combined with endocrine therapy is limited. Whether salvage radiotherapy plus endocrine therapy remains inconclusive. We performed a systematic review and meta-analysis of existing randomized controlled trials to evaluate the efficacy and safety of salvage radiotherapy combined with endocrine therapy in patients with biochemical recurrence after radical prostatectomy.

Methods

A systematic search of PubMed, EMBASE, and the Cochrane library was performed for articles published between January 1, 2012 and October 10, 2022. Data were analyzed using Review Manager 5.4.1 (Cochrane Collaboration Software). Main outcome and measures included biochemical progression-free survival (bPFS), metastasis free survival (MFS), overall survival (OS), and Grade 3 or higher adverse events (3+AEs), including acute and late adverse events.

Results

In this systematic review and meta-analysis, 4 randomized controlled studies enrolling 2731 male (1374 of whom received SRT combined with endocrine therapy and 1357 controls) met the inclusion criteria. SRT combined with endocrine therapy were related to significantly improve bPFS (HR=0.52; 95% CI: 0.46 0.59; p<0.00001) and MFS (HR=0.75; 95% CI: 0.64 0.88; p<0.001). Compared with SRT alone, the combination therapy tended to be associated with prolong OS (HR=0.83; 95% CI: 0.69-1.01; p=0.06), but not statistically significant. At early follow-up, the risk of acute AEs was comparable in the two groups (RR=1.04; 95% CI: 0.22-4.85). However, the risk of late AEs was higher in the combination group at later follow-up (RR=1.33; 95% CI: 1.09-1.62).

Conclusions

This systematic review and meta-analysis found superior efficacy associated with adding endocrine therapy to SRT compared with SRT alone in patients with biochemical recurrence after RP. Additional endocrine therapy is safe and feasible for patients with biochemical recurrence after RP.

Systematic review registration

https://www.crd.york.ac.uk/prospero, identifier (CRD42022365432).
Occult hepatitis B in patients with cancer during immunotherapy with or without chemotherapy: A real-life retrospective single-center cohort study
Authors: Lasagna Angioletta , Albi Giuseppe , Maserati Renato , Zuccarini Andrea , Quaccini Mattia , Baldanti Fausto , Sacchi Paolo , Bruno Raffaele , Pedrazzoli Paolo
Published: January 2023

Introduction

Few data about the safety of immune checkpoint inhibitors (ICIs) in the patients with solid tumor with Occult Hepatitis B Virus (OBI) are available. According to the Taormina Workshop on Occult HBV Infection Faculty Members we defined as potential-OBI (pOBI) the HBV DNA negativity with anti-hepatitis B core antibody (anti-HBc) positivity (pOBI seropositive), and the patients with HBsAg–negative and anti-HBc–negative and Hepatitis B surface antibody (anti-HBs)–negative are defined pOBI seronegative. The aim of this study is to investigate the prevalence of OBI in patients with solid tumors undergoing ICIs with or without chemotherapy and the incidence of reactivation (HBVr).

Methods

We retrospectively enrolled all HBsAg negative subjects who had received ICIs for at least three months. HBsAg and HBV DNA levels were repeated every 3 months until the end of the study and/or in case of ALT alterations. A univariate analysis was conducted in order to study for each variable available its ability to distinguish a potential OBI seropositive patient from a seronegative one.

Results

150 patients in our Oncology Unit were eligible. One hundred and seventeen patients (78%) received ICI as monotherapy, whereas 33 patients (22%) were treated with chemo-immunotherapy. The mainly used drugs for the ICI monotherapy were Pembrolizumab (47%), Nivolumab (33%) and Atezolizumab (11%). The prevalence of pOBI seropositive patients was 25.3%. We did not observe alterations of liver biochemistry nor HBVr.

Discussion

This study highlights that about a quarter of our population had a potential occult hepatitis B. Immunotherapy might be considered as low risk of reactivation, regardless of the potential presence of episomal covalently closed circular DNA (cccDNA) in the liver, but the correct management still represents a challenge for oncologists and hepatologists.
Relationship between polymorphisms in homologous recombination repair genes RAD51 G172T、XRCC2 & XRCC3 and risk of breast cancer: A meta-analysis
Authors: Yu Jiayang , Wang Chun-Guang
Published: January 2023

Background

Genetic variability in DNA double-strand break repair genes such as RAD51 gene and its paralogs XRCC2、XRCC3 may contribute to the occurrence and progression of breast cancer. To obtain a complete evaluation of the above association, we performed a meta-analysis of published studies.

Methods

Electronic databases, including PubMed, EMBASE, Web of Science, and Cochrane Library, were comprehensively searched from inception to September 2022. The Newcastle-Ottawa Scale (NOS) checklist was used to assess all included non-randomized studies. Odds ratios (OR) with 95% confidence intervals (CI) were calculated by STATA 16.0 to assess the strength of the association between single nucleotide polymorphisms (SNPs) in these genes and breast cancer risk. Subsequently, the heterogeneity between studies, sensitivity, and publication bias were performed. We downloaded data from The Cancer Genome Atlas (TCGA) and used univariate and multivariate Cox proportional hazard regression (CPH) models to validate the prognostic value of these related genes in the R software.

Results

The combined results showed that there was a significant correlation between the G172T polymorphism and the susceptibility to breast cancer in the homozygote model (OR= 1.841, 95% CI=1.06–3.21, P=0.03). Furthermore, ethnic analysis showed that SNP was associated with the risk of breast cancer in Arab populations in homozygous models (OR=3.52, 95% CI=1.13-11.0, P= 0.003). For the XRCC2 R188H polymorphism, no significant association was observed. Regarding polymorphism in XRCC3 T241M, a significantly increased cancer risk was only observed in the allelic genetic model (OR=1.05, 95% CI= 1.00–1.11, P=0.04).

Conclusions

In conclusion, this meta-analysis suggests that Rad51 G172T polymorphism is likely associated with an increased risk of breast cancer, significantly in the Arab population. The relationship between the XRCC2 R188H polymorphism and breast cancer was not obvious. And T241M in XRCC3 may be associated with breast cancer risk, especially in the Asian population.
Geographic variation in malignant cardiac tumors and their outcomes: SEER database analysis
Authors: Rahouma Mohamed , Khairallah Sherif , Dabsha Anas , Baudo Massimo , El-Sayed Ahmed Magdy M. , Gambardella Ivancarmine , Lau Christopher , Esmail Yomna M. , Mohamed Abdelrahman , Girardi Leonard , Gaudino Mario , Lorusso Roberto , Mick Stephanie L.
Published: January 2023

Introduction

Primary malignant cardiac tumors (PMCTs) are rare. Geographical distribution has been demonstrated to affect cancer outcomes, making the reduction of geographical inequalities a major priority for cancer control agencies. Geographic survival disparities have not been reported previously for PMCT and the aim of this study is to compare the prevalence and the long-term survival rate with respect to the geographic location of PMCTs using the Surveillance, Epidemiology, and End Results (SEER) research plus data 17 registries between 2000 and 2019.

Methods

The SEER database was queried to identify geographic variation among PMCTs. We classified the included states into 4 geographical regions (Midwest, Northeast, South and West regions) based on the U.S. Census Bureau-designated regions and divisions. Different demographic and clinical variables were analyzed and compared between the four groups. Kaplan Meier curves and Cox regression were used for survival assessment.

Results

A total of 563 patients were included in our analysis. The median age was 53 years (inter-quartile range (IQR): 38 - 68 years) and included 26, 90, 101, and 346 patients from the Midwest, Northeast, South, and West regions respectively. Sarcoma represented 65.6% of the cases, followed by hematological tumors (26.2%), while mesothelioma accounted for 2.1%. Treatment analysis showed no significant differences between different regions. Median overall survival was 11, 21, 13, and 11 months for Midwest, Northeast, South and West regions respectively and 5-year overall survival was 22.2%, 25.4%, 14.9%, and 17.6% respectively. On multivariate Cox regression, significant independent predictors of late overall mortality among the entire cohort included age (Hazard Ratio [HR] 1.028), year of diagnosis (HR 0.967), sarcoma (HR 3.36), surgery (HR 0.63) and chemotherapy (HR 0.56).

Conclusion

Primary malignant cardiac tumors are rare and associated with poor prognosis. Sarcoma is the most common pathological type. Younger age, recent era diagnosis, surgical resection, and chemotherapy were the independent predictors of better survival. While univariate analysis revealed that patients in the South areas had a worse survival trend compared to other areas, geographic disparity in survival was nullified in multivariate analysis.
Impact of MRI resolution for Linac-based stereotactic radiosurgery
Authors: Huang Yimei , Liang Evan , Schaff Eric M. , Zhao Bo , Snyder Karen C. , Chetty Indrin J. , Shah Mira M. , Siddiqui Salim M.
Published: January 2023

Objective

Magnetic resonance imaging (MRI) is a standard imaging modality in intracranial stereotactic radiosurgery (SRS) for defining target volumes. However, wide disparities in MRI resolution exist, which could directly impact accuracy of target delineation. Here, sequences with various MRI resolution were acquired on phantoms to evaluate the effect on volume definition and dosimetric consequence for cranial SRS.

Materials/Methods

Four T1-weighted MR sequences with increasing 3D resolution were compared, including two Spin Echo (SE) 2D acquisitions with 5mm and 3mm slice thickness (SE5mm, SE3mm) and two gradient echo 3D acquisitions (TFE, BRAVO). The voxel sizes were 0.4×0.4×5.0, 0.5×0.5×3.0, 0.9×0.9×1.25, and 0.4×0.4×0.5 mm3, respectively. Four phantoms with simulated lesions of different shape and volume (range, 0.53–25.0 cm3) were imaged, resulting in 16 total sets of MRIs. Four radiation oncologists provided contours on individual MR image set. All observer contours were compared with ground truth, defined on CT image according to the absolute dimensions of the target structure, using Dice similarity coefficient (DSC), Hausdorff distance (HD), mean distance-to-agreement (MDA), and the ratio between reconstructed and true volume (Ratiovol). For dosimetric consequence, SRS plans targeting observer volumes were created. The true Paddick conformity index (CIpaddicktrue), calculated with true target volume, was correlated with quality of observer volume.

Results

All measures of observer contours improved as increasingly higher MRI resolution was provided from SE5mm to BRAVO. The improvement in DSC, HD and MDA was statistically significant (p<0.01). Dosimetrically, CIpaddicktrue strongly correlated with DSC of the planning observer volume (Pearson’s r=0.94, p<0.00001).

Conclusions

Significant improvement in target definition and reduced inter-observer variation was observed as the MRI resolution improved, which also improved the quality of SRS plans. Results imply that high resolution 3D MR sequences should be used to minimize potential errors in target definition, and multi-slice 2D sequences should be avoided.
Spatial distribution of supratentorial diffuse gliomas: A retrospective study of 990 cases
Authors: Li Gen , Yin Chuandong , Zhang Chuanhao , Xue Bowen , Yang Zuocheng , Li Zhenye , Pan Yuesong , Hou Zonggang , Hao Shuyu , Yu Lanbing , Ji Nan , Gao Zhixian , Deng Zhenghai , Xie Jian
Published: January 2023

Background

Gliomas distribute unevenly in the supratentorial brain space. Many factors were linked to tumor locations. This study aims to describe a more detailed distributing pattern of these tumors with age and pathological factors concerned.

Methods

A consecutive series of 990 adult patients with newly-diagnosed supratentorial diffuse gliomas who underwent resection in Beijing Tiantan Hospital between January 2013 and January 2017 were retrospectively reviewed. For each patient, the anatomic locations were identified by the preoperative MRI, and the pathological subtypes were reviewed for histological grade and molecular status (if any) from his medical record. The MNI template was manually segmented to measure each anatomic location’s volume, and its invaded ratio was then adjusted by the volume to calculate the frequency density. Factors of age and pathological subtypes were also compared among locations.

Results

The insulae, hippocampi, and corpus callosum were locations of the densest frequencies. The frequency density decreased from the anterior to posterior (frontal - motor region - sensory region - parietal - occipital), while the grade (p < 0.0001) and the proportion of IDH-wt (p < 0.0001) increased. More tumors invading the right basal ganglion were MGMT-mt (p = 0.0007), and more of those invading the left frontal were TERT-wt (p = 0.0256). Age varied among locations and pathological subtypes.

Conclusions

This study demonstrated more detailed spatial disproportions of supratentorial gliomas. There are potential interactions among age, pathological subtypes, and tumor locations.
Human epidermal growth factor receptor 2 expression in women with uterine cervix adenocarcinoma from Appalachian Kentucky
Authors: Kunos Charles A. , Fabian Denise , Piecoro Dava W. , Napier Dana , Miller Rachel W. , Ueland Frederick R.
Published: January 2023

Introduction

High-risk human epidermal growth factor receptor 2 (HER2)-positive adenocarcinomas associate with early recurrence and death, prompting consideration of novel radiotherapeutic options like a trastuzumab-linked thorium-227 alpha-particle emitting radionuclide.

Methods

We conducted a retrospective pilot biomarker study of uterine cervix cancers among patients in Appalachian Kentucky, to characterize an exploitable triage biomarker like HER2 expression before starting a prospective phase 0 trial.

Results

Most (60%) adenocarcinomas showed HER2 cell-surface overexpression, whereas squamous cell carcinomas (4%) did not do so.

Discussion

Further validation tests of HER2 expression as a triage biomarker for radiopharmaceutical selection are warranted.
Chinese herbal medicine combined with western medicine for the treatment of type 2 diabetes mellitus with hyperuricemia: A systematic review and meta-analysis
Authors: Liu Hongyan , Peng Sihan , Yuan Haipo , He Yuchi , Tang Jiao , Zhang Xiyu
Published: January 2023

Background: Chinese herbal medicine (CHM) has the advantage of being safe and effective and has been widely used in clinical practice for the treatment of type 2 diabetes mellitus (T2DM) with hyperuricemia (HUA), but its overall efficacy and safety remain unclear. This study aimed to evaluate the efficacy and safety of CHM for the treatment of T2DM with HUA based on randomized controlled trials (RCTs) to provide clinical evidence.

Methods: The protocol evaluated in this study is registered with PROSPERO (CRD42022351519). As of November 2022, eight databases were searched, and RCTs of CHM for the treatment of T2DM with HUA were included. Outcome indicators observed included fasting blood glucose (FBG), 2-h postprandial glucose (2hPG), glycated hemoglobin (HbA1c), uric acid (UA), triglycerides (TG), total cholesterol (TC), overall effectiveness, and adverse events. Utilizing Review Manager 5.4, Stata V14.0, and GRADEpro, the included studies were evaluated, and the quality of the evidence was determined.

Results: 18 RCTs covering 1,311 patients were included in this study. The results of the study demonstrated that the combination of CHM and western medicine (WM) was more effective in treating patients with T2DM with HUA than WM alone, with significant improvements in FBG (weighted mean differences (WMD) = −0.60.95% confidence interval (CI) [−0.81, −0.40], p < 0.00001), 2hPG (WMD = −1.12.95% CI [−1.64, −0.60], p < 0.0001), HbA1c (WMD = −0.80.95% CI [−1.04, −0.56], p < 0.00001), UA (WMD = −53.47.95% CI [−67.45, −39.48], p < 0.00001), TG (WMD = −0.56.95% CI [−0.74, −0.38], p < 0.00001), TC (WMD = −0.49.95% CI [−0.65, −0.33], p < 0.00001), and overall effective rate (risk ratio (RR) = 1.29.95%CI [1.13, 1.48], p = 0.0002). The quality of evidence for all outcomes was low.

Conclusion: Compared with WM alone, the combination of CHM and WM was more effective in treating patients with T2DM with HUA, with significant improvements in glucose metabolism, uric acid, and lipids. However, further evaluation by high−quality RCT results is needed due to the low quality and high heterogeneity of the evidence.

Systematic Review Registration: [https://systematicreview.gov/], identifier [CRD42022351519].
RADFNet: An infrared and visible image fusion framework based on distributed network
Authors: Feng Siling , Wu Can , Lin Cong , Huang Mengxing
Published: January 2023

Introduction

The fusion of infrared and visible images can improve image quality and eliminate the impact of changes in the agricultural working environment on the information perception of intelligent agricultural systems.

Methods

In this paper, a distributed fusion architecture for infrared and visible image fusion is proposed, termed RADFNet, based on residual CNN (RDCNN), edge attention, and multiscale channel attention. The RDCNN-based network realizes image fusion through three channels. It employs a distributed fusion framework to make the most of the fusion output of the previous step. Two channels utilize residual modules with multiscale channel attention to extract the features from infrared and visible images, which are used for fusion in the other channel. Afterward, the extracted features and the fusion results from the previous step are fed to the fusion channel, which can reduce the loss in the target information from the infrared image and the texture information from the visible image. To improve the feature learning effect of the module and information quality in the fused image, we design two loss functions, namely, pixel strength with texture loss and structure similarity with texture loss.

Results and discussion

Extensive experimental results on public datasets demonstrate that our model has superior performance in improving the fusion quality and has achieved comparable results over the state-of-the-art image fusion algorithms in terms of visual effect and quantitative metrics.
Plant production of a virus-like particle-based vaccine candidate against porcine reproductive and respiratory syndrome
Authors: VanderBurgt Jordan T. , Harper Ondre , Garnham Christopher P. , Kohalmi Susanne E. , Menassa Rima
Published: January 2023

Porcine reproductive and respiratory syndrome (PRRS) is a disease leading to spontaneous abortions and stillbirths in sows and lowered life quality and expectancy in growing pigs. PRRS is prevalent worldwide and has significant economic impacts to swine industries around the globe. Co-expression of the two most abundant proteins in the viral envelope, the matrix protein (M) and glycosylated protein 5 (GP5), can produce a neutralizing immune response for the virus providing a potentially effective subunit vaccine against the disease, but these proteins are difficult to express. The goal of this research was to display antigenic portions of the M and GP5 proteins on the surface of tobacco mosaic virus-like particles. A modified tobacco mosaic virus coat protein (TMVc) was transiently expressed inNicotiana benthamianaleaves and targeted to three subcellular compartments along the secretory pathway to introduce glycosylation patterns important for M-GP5 epitope immunogenicity. We found that accumulation levels in the apoplast were similar to the ER and the vacuole. Because glycans present on plant apoplastic proteins are closest to those present on PRRSV proteins, a TMVc-M-GP5 fusion construct was targeted to the apoplast and accumulated at over 0.5 mg/g of plant fresh weight. TMVc virus-like particles self-assembled in plant cells and surface-displayed the M-GP5 epitope, as visualized by transmission electron microscopy and immunogold localization. These promising findings lay the foundation for immunogenicity and protective-immunity studies in animals to examine the efficacy of this vaccine candidate as a measure to control PRRS.
Positive reputation for altruism toward future generations regardless of the cost for current others
Authors: Inoue Yukako , Mifune Nobuhiro , Saijo Tatsuyoshi
Published: January 2023

Recently, altruism toward future generations (future altruism) has become a hot research topic. Although future altruism has been observed in several previous experiments, it is not yet clear when and why people are more likely to engage in future altruism. Drawing upon the empirical literature of reputation and cooperation, we predicted that future altruism brings reputational disadvantages. Accordingly, we investigated whether future altruism was evaluated positively or negatively by others in the current generation in two vignette studies (total N = 1,237). Contrary to our initial prediction, we found that future altruism was positively evaluated even when it decreased the payoff of the members of the current generation. The difference in the evaluation of future altruism, as opposed to unsustainable current-generation focused behavior, was most pronounced when people do not know how a future altruist allocates rewards among individuals in the current generation. However, the positive evaluation of future altruism did not stem from the expectation that future altruists would also be altruistic toward the current generation. These results indicated that reputational benefits (i.e., positive reputation from others in the current generation) promote future altruism.