bluuebunny/crossref_metadata_2025 huggingface dataset:
Obsessive-Compulsive Disorder with Psychotic Features: Is It a Clinical Entity?
Authors: Okamura Yasushi , Murahashi Yuki , Umeda Yuna , Misumi Toshihiro , Asami Takeshi , Itokawa Masanari , Harima Hirohiko , Mizuno Masafumi , Matsunaga Hisato , Hishimoto Akitoyo
Published: September 2022

(1) Background: Even though the comorbidity of obsessive-compulsive disorder (OCD) and a psychotic disorder (PD), such as schizophrenia, is being increasingly recognized, the impact of this comorbidity on the clinical presentation, including insight into obsessive-compulsive symptoms and the functioning of OCD, remains unclear. (2) Methods: To investigate clinical differences between OCD patients with and without PD, 86 Japanese outpatients who met the DSM-IV-TR criteria for OCD were recruited and divided into two groups: 28 OCD patients with PD, and 58 OCD patients without PD. The two groups were cross-sectionally compared in terms of their sociodemographic profiles and clinical characteristics, including the DSM-IV-TR insight specifier and the Global Assessment of Functioning (GAF). (3) Results: The results showed that OCD patients with PD scored lower on both the insight and GAF assessments. (4) Conclusions: The present study suggests that comorbid PD in OCD is a clinical entity.
Prospective Analysis of the Temporal Relationship between Psychological Distress and Atopic Dermatitis in Female Adults: A Preliminary Study
Authors: Birdi Gurkiran , Larkin Michael , Knibb Rebecca C.
Published: September 2022

Atopic dermatitis (AD) has been associated with psychological distress, but few studies have examined the causal relationships. This study aimed to investigate whether stress, anxiety, or depression could lead to an increase in AD severity or vice versa in adults using a longitudinal study design. Daily diaries measuring psychological stress were completed over four weeks; validated questionnaires measuring stress, anxiety, depression, and AD severity were completed weekly for twelve weeks. Thirty-six participants (all female, aged 18–46 years) were recruited; complete data were returned from 19. Stress and disease severity were significantly correlated when measured daily and weekly for the duration of the study. Cross-lagged panel model (CLPM) analyses identified that for the weekly measures, stress, anxiety, and depression on week X significantly predicted disease severity on week X + 1. Disease severity on week X also predicted psychological stress, anxiety and depression on week X + 1 for the majority of the twelve weeks. There appears to be a bi-directional relationship between stress, anxiety and depression, and AD severity in women. High levels of distress should be identified so that optimum management strategies can be implemented to reduce the risk of increased AD severity and the resulting impact severity might have on psychological wellbeing.
Decision Conflicts in Clinical Care during COVID-19: A Multi-Perspective Inquiry
Authors: Haier Joerg , Beller Johannes , Adorjan Kristina , Bleich Stefan , de Greck Moritz , Griesinger Frank , Heppt Markus , Hurlemann René , Mees Soeren Torge , Philipsen Alexandra , Rohde Gernot , Schilling Georgia , Trautmann Karolin , Combs Stephanie E. , Geyer Siegfried , Schaefers Juergen
Published: September 2022

Background: The early COVID-19-pandemic was characterized by changes in decision making, decision-relevant value systems and the related perception of decisional uncertainties and conflicts resulting in decisional burden and stress. The vulnerability of clinical care professionals to these decisional dilemmas has not been characterized yet. Methods: A cross-sectional questionnaire study (540 patients, 322 physicians and 369 nurses in 11 institutions throughout Germany) was carried out. The inclusion criterion was active involvement in clinical treatment or decision making in oncology or psychiatry during the first year of COVID-19. The questionnaires covered five decision dimensions (conflicts and uncertainty, resources, risk perception, perception of consequences for clinical processes, and the perception of consequences for patients). Data analysis was performed using ANOVA, Pearson rank correlations, and the Chi²-test, and for inferential analysis, nominal logistic regression and tree classification were conducted. Results: Professionals reported changes in clinical management (27.5%) and a higher workload (29.2%), resulting in decisional uncertainty (19.2%) and decisional conflicts (22.7%), with significant differences between professional groups (p < 0.005), including anxiety, depression, loneliness and stress in professional subgroups (p < 0.001). Nominal regression analysis targeting “Decisional Uncertainty” provided a highly significant prediction model (LQ p < 0.001) containing eight variables, and the analysis for “Decisional Conflicts” included six items. The classification rates were 64.4% and 92.7%, respectively. Tree analysis confirmed three levels of determinants. Conclusions: Decisional uncertainty and conflicts during the COVID-19 pandemic were independent of the actual pandemic load. Vulnerable professional groups for the perception of a high number of decisional dilemmas were characterized by individual perception and the psychological framework. Coping and management strategies should target vulnerability, enable the handling of the individual perception of decisional dilemmas and ensure information availability and specific support for younger professionals.
Elucidating the Molecular Responses to Waterlogging Stress in Cucumis melo by Comparative Transcriptome Profiling
Authors: Zhang Huanxin , Li Guoquan , Yan Chengpu , Zhang Xinlong , Cao Na , Le Meiwang , Hu Xinlong , Zhu Fanghong , Liu Wenge
Published: September 2022

Waterlogging is a serious abiotic stressor that drastically hinders the growth and productivity of melon (Cucumis melo) around the world, due to the reduction in available oxygen levels in the waterlogged tissues. However, the mechanism underlying the responses to waterlogging stress in melon is largely unknown. In this study, physiological and transcriptome data of the waterlogging-sensitive accession ‘L39’ and the waterlogging-tolerant accession ‘L45’ were investigated under conditions of normal water supply and waterlogging stress. The results showed that ‘L45’ exhibited higher chlorophyll contents and lower REL (relative electrolyte leakage) and MDA (malondialdehyde) contents compared with ‘L39’ under waterlogging stress. Additionally, waterlogging stress only led to the stomatal closure and chloroplast damage of ‘L39’. In total, 1748 genes were differentially expressed in the leaves of waterlogging-stressed ‘L45’ compared with control, whereas 3178 genes were differentially expressed in ‘L39’. Further analysis indicated that genes related to chlorophyll synthesis and photosynthesis were more depressed in ‘L39’, while sugar cleavage, glycolysis, and fermentation genes were highly induced in ‘L39’ compared with ‘L45’. The expression of genes involved in ROS (reactive oxygen species) scavenging and hormone signaling significantly differed between ‘L39’ and ‘L45’ in their response to waterlogging stress. Moreover, a total of 311 differentially expressed transcription factors were waterlogging-responsive, among which members of the ERF (ethylene response factor), bHLH (basic helix-loop-helix), and WRKY families might play crucial roles in waterlogging tolerance in melon. This study unraveled the molecular responses to waterlogging stress in melon and could provide helpful candidate genes for further molecular breeding of waterlogging-tolerant melon varieties.
Peanut Root Exudates Suppress Fusarium solani and Modulate the Microbial Community Structure of Rhizosphere in Grape Replant Soil
Authors: Zhang Jiale , Liu Qianwen , Li Kun , Ma Li
Published: September 2022

Replant disease significantly hinders the development of the grape industry, and the imbalance of the rhizosphere microecological environment is one of the fundamental reasons hindering grape replants. Peanut is a common intercropping crop, and whether the root exudates of peanut can alleviate grape replant obstacles is still unknown. In this study, the effects of exogenous peanut root exudates on replanting grapevine growth, and the microbial community structure of grapevine replant soils were studied. The results showed that peanut root exudates could promote the growth of replanting grapevine seedlings; enhance root vigor and SOD activity, increasing 55.18% and 95.71%, respectively; and reduce the MDA content of root, decreasing 31.10%. After peanut exudate treatment, the growth of Fusarium solanum, an important harmful fungus that is an obstacle to grape replant, was inhibited. The relative abundances of Gaiella in bacteria and Cystobasidium and Mortierella in fungi increased, and the potential pathogen fungi Fusicolla decreased. Peanut root exudates also modified the soil bacterial and fungal community in a certain range and increased the interaction among the bacteria of grapevine rhizosphere soil. However, they loosened the interaction among fungi. There are extensive mutualistic interactions among bacteria or fungi in grape rhizosphere assemblages after peanut exudates treatment. Therefore, peanut root exudates might be helpful in changing the soil microbial environment and alleviating the grape replanting obstacle.
Current Trends in Organic Vegetable Crop Production: Practices and Techniques
Authors: Fernández Juan A. , Ayastuy Miren Edurne , Belladonna Damián Pablo , Comezaña María Micaela , Contreras Josefina , de Maria Mourão Isabel , Orden Luciano , Rodríguez Roberto A.
Published: September 2022

Organic farming is a holistic production management system that promotes and enhances agroecosystem health, including biodiversity, biological cycles and soil biological activity, and consequently, it is an efficient and promising approach for sustainable agriculture within a circular and green economy. There has been a rise in the consumption of organic vegetables in the last years because of their organoleptic properties, higher nutritive value and lower risk of chemical residues harmful to health. The recent scientific evidence regarding the use of the major elements responsible for organic vegetable crop production indicates plant material, soil management and crop nutrition, soil disinfection, crop management and pest, disease and weed management. These techniques are the focus of this study. In general, the main outcomes of this review demonstrate that a great effort of innovation and research has been carried out by industry, researchers and farmers in order to reduce the environmental impact of the established and innovative horticultural practices while satisfying the requirements of consumers. However, research-specific studies should be carried out in different farming systems and pedoclimatic conditions to achieve the highest efficiency of these horticultural practices.
Identification and Characterization of Trihelix Transcription Factors and Expression Changes during Flower Development in Pineapple
Authors: Wang Jing , Ouyang Yanwei , Wei Yongzan , Kou Jingjing , Zhang Xiaohan , Zhang Hongna
Published: September 2022

Trihelix transcription factors with helix–loop–helix–loop–helix characteristics are essential in plant growth, development, and response to adversity. Several potential functions of Trihelix have been studied in multiple plant species, but little is known about the features and functions of Trihelix genes in pineapple. In this study, 23 Trihelix members were identified and classified into 5 groups and were unevenly distributed in 17 chromosomes of pineapple. The results revealed that six fragment duplication events and one tandem duplication event were found through gene duplication analysis. Moreover, 4, 10, 21, and 23 homologous gene pairs were found between pineapple and Arabidopsis, grape, banana, and rice, respectively. The promoters of Trihelix have many cis-elements, especially in light and hormone response. The expression characteristics of AcTrihelix members showed obvious tissue specificities in different tissues. The expressions of AcTrihelix3, AcTrihelix8, AcTrihelix16, AcTrihelix19, AcTrihelix20, and AcTrihelix23 were maintained at high levels during the late stage of flower bud development. In floral organs, the expression of different members was very different. In conclusion, some AcTrihelix members may play important roles during the floral development of pineapple, and they provide resources for further studies of the function of Trihelix and the molecular mechanism during pineapple flower formation.
Comparative Transcriptome Analysis Reveals the Molecular Mechanism of UV-B Irradiation in Promoting the Accumulation of Phenolic Compounds in Wounded Carrot
Authors: Zhen Wenna , Tu Yi , Lin Zihan , Xu Xinxin , Fu Maorun , Han Cong
Published: September 2022

Ultraviolet B (UV-B) irradiation has been demonstrated to play a synergistic effect with wounding in enhancing the accumulation of phenolic antioxidants in carrots. However, little is known about the molecular mechanisms of UV-B treatment on wounded carrots. In this study, wounded carrots were exposed to different doses of UV-B light, then stored at 15 °C for 2 d. The results showed that the content of phenolic compounds in 1 KJ m−2 UV-B–treated samples was 415% and 247% higher than that of whole and wounded carrots, respectively. Based on this, 1 KJ m−2 was selected as the optimal dose of UV-B treatment and used for further analysis. In addition, UV-B treatment greatly enhanced the enzyme activity of phenylalanine ammonia lyase (PAL) and the contents of phenolic profiles, especially 3-O-caffeoylquinic acid (3-CQA). Transcriptome analysis found that UV-B treatment could accelerate the KEGG pathways involved in signal transduction and secondary substance metabolism. The differentially expressed genes (DEGs) in phenolics biosynthesis–related metabolic processes of shikimate pathway and phenylpropanoid biosynthesis were extensively upregulated by UV-B treatment. Our results provided fundamental information for a better understanding of the molecular regulation mechanism of UV-B treatment in promoting the accumulation of phenolic compounds in wounded carrots.
Effects of Neem Seed Extract on Nitrate and Oxalate Contents in Amaranth Fertilized with Mineral Fertilizer and Cricket Frass
Authors: Sriraj Pranee , Toomsan Banyong , Butnan Somchai
Published: September 2022

A vegetable’s high antinutrients, nitrate (NO3−) and oxalate, could be remediated by neem seed extract. The combined use of neem seed extract with mineral fertilizer and cricket frass was conducted to evaluate their effects on amaranth’s tissue NO3− and oxalic acid contents by inhibiting nitrification. The effects of five soil amendments were investigated: unamended, mineral fertilizer, and three rates of cricket frass (3.125 Mg ha−1, 6.25 Mg ha−1, and 12.5 Mg ha−1), combined with two rates of neem seed extract: without (−Nm) and with (+Nm) extract. Only the neem extract applied to soils receiving mineral fertilizers decreased soil tissue NO3−−N contents (0.82 g kg−1 for −Nm vs. 0.62 g kg−1 for +Nm). The oxalic acid content of amaranth decreased with mineral fertilizer (0.60 and 0.46 g kg−1 for −Nm and +Nm, respectively), yet increased with the higher rates of cricket frass (1.42–1.52 g kg−1 for −Nm, and 1.23–1.51 g kg−1 for +Nm) compared to the unamended soil (1.05 and 1.00 g kg−1 for −Nm and +Nm). Cations, including K, Ca, Mg, and Na derived from cricket frass, may enhance biosynthesis and the accumulation of oxalic acid. The neem seed extract decreased the tissue’s oxalic contents regardless of soil amendments.
Deaths of Despair: A Scoping Review on the Social Determinants of Drug Overdose, Alcohol-Related Liver Disease and Suicide
Authors: Beseran Elisabet , Pericàs Juan M. , Cash-Gibson Lucinda , Ventura-Cots Meritxell , Porter Keshia M. Pollack , Benach Joan
Published: September 2022

Background: There is a lack of consensus on the social determinants of Deaths of Despair (DoD), i.e., an increase in mortality attributed to drug overdose, alcohol-related liver disease, and suicide in the United States (USA) during recent years. The objective of this study was to review the scientific literature on DoD with the purpose of identifying relevant social determinants and inequalities related to these mortality trends. Methods: Scoping review focusing on the period 2015–2022 based on PubMed search. Articles were selected according to the following inclusion criteria: published between 1 January 2000 and 31 October 2021; including empirical data; analyzed DoD including the three causes defined by Case and Deaton; analyzed at least one social determinant; written in English; and studied DoD in the USA context only. Studies were excluded if they only analyzed adolescent populations. We synthesized our findings in a narrative report specifically addressing DoD by economic conditions, occupational hazards, educational level, geographical setting, and race/ethnicity. Results: Seventeen studies were included. Overall, findings identify a progressive increase in deaths attributable to suicide, drug overdose, and alcohol-related liver disease in the USA in the last two decades. The literature concerning DoD and social determinants is relatively scarce and some determinants have been barely studied. However different, however, large inequalities have been identified in the manner in which the causes of death embedded in the concept of DoD affect different subpopulations, particularly African American, and Hispanic populations, but blue collar-whites are also significantly impacted. Low socioeconomic position and education levels and working in jobs with high insecurity, unemployment, and living in rural areas were identified as the most relevant social determinants of DoD. Conclusions: There is a need for further research on the structural and intermediate social determinants of DoD and social mechanisms. Intersectional and systemic approaches are needed to better understand and tackle DoD and related inequalities.
The Effect of Organizational Trust on Turnover Intention of Rural Kindergarten Teachers: The Mediating Role of Teaching Efficacy and Job Satisfaction
Authors: Zhao Yan , Lu Zhenjie , Cheng Xiulan , Li Jiaqi
Published: September 2022

Recent studies have focused on turnover among rural kindergarten teachers. However, none of these studies have shown a clear connection between turnover intention and organizational trust, although there are studies in other areas showing that organizational trust can affect turnover intention. Drawing on a sample of 330 kindergarten teachers in rural areas, this study explores the mechanism of influence between organizational trust and turnover intention with teaching efficacy and job satisfaction as mediators. We found that organizational trust negatively impacted teachers’ turnover intention, and this relationship was mediated by a significant chain mediating effect of teaching efficacy and job satisfaction. The findings enrich knowledge about turnover among rural kindergarten teachers and inspire us to create a more supportive organizational environment against the backdrop of the COVID-19 pandemic to improve job satisfaction and alleviate turnover among rural kindergarten teachers.
Evaluation of the Impact of Activated Biochar-Manure Compost Pellet Fertilizer on Volatile Organic Compound Emissions and Heavy Metal Saturation
Authors: Kim Minsoo , Jeong Changyoon , Kim Minjeong , Nam Joohee , Shim Changki , Shin Joungdu
Published: September 2022

For this experiment, pelletized activated biochar made of rice hullsor palm bark with swine manure compost was prepared to demonstrate the significant benefits of applying activated biochar-manure compost pellet fertilizer (ABMCP) inmitigating volatile organic compounds (VOCs), odor emission, and heavy metal saturation. Morphology and surface area analysis indicated that the activated rice hull biochar-manure compost pellet (ARP) had a significantly lower surface area, porous volume, and Fe content the activated palm biochar-manure compost pellet (APP). However, the ARP presented great potential to mitigate VOCs and odorant emissions. Our results indicated that the ARP reduced total reduced sulfur (TRS) and volatile fatty acids (VFAs) emissions by 69% and 93%, respectively. Heavy metals such as Pb, As, and Cd were not detected in the leachates fromthe ARP, APP, and swine manure compost. These results suggest that ABMCP can be a potential adsorbent to control VOCs and odorant emissions andpromote sustainable swine manure management and agricultural application.
The Impact of Airline’s Smart Work System on Job Performance of Cabin Crew
Authors: Jung Yongjin , Kim Haeok Liz , Hyun Sunghyup Sean
Published: September 2022

Extant studies in medical and educational fields have demonstrated that employees’ device use (smartphones, tablet PCs, etc.) can enhance job performance. Correspondingly, global airline companies have made substantial investments to enhance passenger services. An earlier study examined the impact of flight attendants’ technology usage on job satisfaction by investigating the causal relationship between the benefits of tablet PC use, job performance, and its consequences. Based on the literature review, four advantages of technology use were derived: (1) efficiency, (2) convenience, (3) service effectiveness, and (4) pride. Additionally, three consequences of job satisfaction were derived: (1) team performance, (2) organizational commitment, and (3) turnover intention. Empirical data were collected from 208 flight attendants working for a South Korean airline, which provided tablet PCs for its employees. Data analysis revealed that work efficiency, convenience, and pride had a significant and positive impact on job satisfaction. However, flight preparation did not show a similar impact. This study is the first to investigate the benefits of using technology in the airline industry. Furthermore, it examined the convergence of airline management and information technology. The findings provide managerial implications for airline companies that are considering providing tablet PCs to flight attendants.
Clinical Assessment of Functional Recovery Following Nerve Transfer for Traumatic Brachial Plexus Injuries
Authors: Tsai Yi-Jung , Hsiao Chih-Kun , Su Fong-Chin , Tu Yuan-Kun
Published: September 2022

Surgical reconstruction and postoperative rehabilitation are both important for restoring function in patients with traumatic brachial plexus injuries (BPIs). The current study aimed to understand variations in recovery progression among patients with different injury levels after receiving the nerve transfer methods. A total of 26 patients with BPIs participated in a rehabilitation training program over 6 months after nerve reconstruction. The differences between the first and second evaluations and between C5–C6 and C5–C7 BPIs were compared. Results showed significant improvements in elbow flexion range (p = 0.001), British Medical Research Council’s score of shoulder flexion (p = 0.046), shoulder abduction (p = 0.013), shoulder external rotation (p = 0.020), quantitative muscle strength, and grip strength at the second evaluation for both groups. C5–C6 BPIs patients showed a larger shoulder flexion range (p = 0.022) and greater strength of the shoulder rotator (p = 0.004), elbow flexor (p = 0.028), elbow extensor (p = 0.041), wrist extensor (p = 0.001), and grip force (p = 0.045) than C5–C7 BPIs patients at the second evaluation. Our results indicated different improvements among patients according to injury levels, with quantitative values assisting in establishing goals for interventions.
Mapping the Way to Good Health: The Interdisciplinary Challenges of Geographers in Medical Research
Authors: Sadler Richard , Larsen Kristian
Published: September 2022

Geography has an important role to play in shaping the direction of medical research. In particular, its tools and theory provide essential understanding to the impacts of place on health behaviors and outcomes. Understanding some of its evolution—particularly into the subfield of medical geography—is therefore useful both for geographers and medical researchers. In this paper, we present some of the debates that geographers have grappled with, the growth of GIS (particularly in the context of medical research), some important methodological considerations that geographers help center, and some recommendations for future work at this nexus. Throughout, we speak from the perspective of geographers who have worked nearly exclusively in the health sciences since obtaining our PhDs.
Effect of COVID-19 Vaccines on Reducing the Risk of Long COVID in the Real World: A Systematic Review and Meta-Analysis
Authors: Gao Peng , Liu Jue , Liu Min
Published: September 2022

The coronavirus disease 2019 (COVID-19) is still in a global pandemic state. Some studies have reported that COVID-19 vaccines had a protective effect against long COVID. However, the conclusions of the studies on the effect of COVID-19 vaccines on long COVID were not consistent. This study aimed to systematically review relevant studies in the real world, and performed a meta-analysis to explore the relationship between vaccination and long COVID. We systematically searched PubMed, Embase, Web of science, and ScienceDirect from inception to 19 September 2022. The PICO (P: patients; I: intervention; C: comparison; O: outcome) was as follows: patients diagnosed with COVID-19 (P); vaccination with COVID-19 vaccines (I); the patients were divided into vaccinated and unvaccinated groups (C); the outcomes were the occurrence of long COVID, as well as the various symptoms of long COVID (O). A fixed-effect model and random-effects model were chosen based on the heterogeneity between studies in order to pool the effect value. The results showed that the vaccinated group had a 29% lower risk of developing long COVID compared with the unvaccinated group (RR = 0.71, 95% CI: 0.58–0.87, p < 0.01). Compared with patients who were not vaccinated, vaccination showed its protective effect in patients vaccinated with two doses (RR = 0.83, 95% CI: 0.74–0.94, p < 0.01), but not one dose (RR = 0.83, 95% CI: 0.65–1.07, p = 0.14). In addition, vaccination was effective against long COVD in patients either vaccinated before SARS-CoV-2 infection/COVID-19 (RR = 0.82, 95% CI: 0.74–0.91, p < 0.01) or vaccinated after SARS-CoV-2 infection/COVID-19 (RR = 0.83, 95% CI: 0.74–0.92, p < 0.01). For long COVID symptoms, vaccination reduced the risk of cognitive dysfunction/symptoms, kidney diseases/problems, myalgia, and sleeping disorders/problems sleeping. Our study shows that COVID-19 vaccines had an effect on reducing the risk of long COVID in patients vaccinated before or after SARS-CoV-2 infection/COVID-19. We suggest that the vaccination rate should be improved as soon as possible, especially for a complete vaccination course. There should be more studies to explore the basic mechanisms of the protective effect of COVID-19 vaccines on long COVID in the future.
To a Green Economy across the European Union
Authors: Ionescu Romeo Victor , Antohi Valentin Marian , Zlati Monica Laura , Georgescu Lucian Puiu , Iticescu Catalina
Published: September 2022

”Is the green economy a real solution to the present economic development?” represents the main question of the present research. The paper deals with assessing the impact of innovation on the green economy and quality of life through policies promoted at the European Union (EU) level. The objectives of the paper are to assess the impact level of the policy implementation across the Member States through the research and development (R&D) process and to identify models for the development of the green economy in Europe. The applied methods are empirical and analytical based on the study of literature, econometric modeling, pivoting econometric models, trend synthesis, prioritization, and staging of policy experimentation in the Member States through data processing and statistical programs. The results consist in obtaining development models for the green economy at the EU and national levels. In conclusion, the paper contributes to the early identification of vulnerabilities that may affect the development of European strategic projects in relation to events and security breaches occurring at the EU level at any given time.
Experiences and Expectations of the Characteristics of Friendly Primary Health Services from the Perspective of Men: A Phenomenological Qualitative Study
Authors: Ab Aziz Muhammad Zikri , Tengku Ismail Tengku Alina , Ibrahim Mohd Ismail , Yaacob Najib Majdi , Mohd Said Zakiah
Published: September 2022

Inadequate men’s engagement with health services may be influenced by unmet needs and demands of the local men’s community. This study aimed to explore men’s experiences with primary health services and their expectations of the characteristics of friendly primary health services, from the perspective of men in Kelantan, Malaysia. A qualitative study using in-depth interviews was conducted with 15 men from six primary health facilities in Kelantan, Malaysia, who were selected based on maximum variation sampling. The data were transcribed and analyzed using the thematic analysis method. The study found that experiences with the existing primary health services were categorized into four subthemes: provision of health services, health promotion delivery, attributes of healthcare providers, and the physical environment of the health facilities. Meanwhile, the expectations of the characteristics of friendly primary health services were categorized into four subthemes: meeting the needs of men in primary health services, approaching men through effective health promotion strategies, standards of a healthcare provider from the viewpoint of men, and a comfortable physical environment for men. Prior experiences hugely influenced men’s expectations of friendly primary health services. Men want these health service characteristics customized to meet their needs, allowing them to use health services with confidence and comfort. Thus, to strengthen primary health services for men, it is essential to comprehend their prior experiences with and expectations of the services.
Effects of Noise Exposure and Mental Workload on Physiological Responses during Task Execution
Authors: Fan Yurong , Liang Jin , Cao Xiaodong , Pang Liping , Zhang Jie
Published: September 2022

Twelve healthy male students were recruited to investigate the physiological response to different noise exposure and mental workload (MW) conditions, while performing multi-attribute task battery (MATB) tasks. The experiments were conducted under three noise exposure conditions, with different sound pressure levels and sharpness. After adaptation to each noise condition, the participants were required to perform the resting test and the MATB task tests with low, medium, and high MW. The electroencephalogram (EEG), electrocardiogram (ECG), and eye movement data were obtained, during the periods when participants were in the resting and task taking state. The results showed that subjects’ physiological responses at rest were unaffected by noise exposure conditions. However, during the execution of MATB tasks, the elevated sound pressure level and increased sharpness were significantly correlated with increased mean pupil diameter and heart rate variability (HRV). These responses suggested that the human body defends itself through physiological regulation when noise causes adverse effects. If the negative effects of noise were more severe, this could damage the body’s health and result in a significant drop in task performance. The elevated mental demands led to increased stress on the subjects, which was reflected in a considerable increase in theta relative power. Either high or low MW was related with reduced saccade amplitude and a decrease in weighted task performance, indicating an inverted U-shaped relationship between workload level and work performance.
Oral Health Care of People with Down Syndrome in Germany
Authors: Schmidt Peter , Suchy Laura C. , Schulte Andreas G.
Published: September 2022

Background: Due to a dearth of information on preventive and supportive oral care for people with Down Syndrome (PDS) in Germany, caregivers of PDS were surveyed on the topic. Methods: An anonymized questionnaire was sent to the 610 members of the German Down Syndrome Association. The study was approved by the ethics committee of Witten/Herdecke University (# 165/2017). Results: The parents/caregivers of 207 PDS responded. These PDS were between 1 and 55 years old (mean age 24.4 years) and included 111 males and 96 females. At their first dental appointment, 40.7% (n = 82) had been younger than three years. Although 78.3% (n = 162) tooth brushed twice daily, only 30.9% (n = 64) brushed for 2–3 min; 84.0% (n = 135) did not use other dental hygiene products apart from toothbrushes. Age-specific differences were found: Although only 30.8% (n = 12) of PDS < 18 years (n = 39) independently performed dental self-care; this was 67.3% (n = 113) for PDS ≥ 18 years (n = 168). A statistically significant (p < 0.001; Chi-Square-Test) difference in toothbrush preferences emerged: While PDS < 18 years mainly used manual toothbrushes, PDS ≥ 18 years preferred electric toothbrushes. In contrast to 72.6% of PDS ≥ 18 years, only 51.3% of PDS < 18 years accepted most types of toothpaste. Conclusions: Age-dependent differences in tooth brushing behaviour became evident within the group of PDS in Germany. Hence, better age-specific, inter-professional dental prophylaxis concepts need to be developed and offered for all age groups of PDS. These concepts should include personalized check-up intervals and hands-on instruction in dental hygiene procedures by dental practitioners.
The Influence of Stigma Perceptions on Employees’ Claims Experiences for Psychological Injuries: Re-Examination of a Cross-Sectional Survey among Australian Police and Emergency Service Personnel
Authors: Sanatkar Samineh , Bartlett Jenn , Harvey Samuel , Counson Isabelle , Lawrence David
Published: September 2022

While a large body of research assessed the contribution of mental health stigma on disclosure, treatment seeking, and recovery, limited research exists seeking to identify the relative contribution of stigma beliefs on workers’ compensation claims for psychological injury. Survey data of ambulance, fire and rescue, police, and state emergency service personnel (N = 1855, aged 45–54 years, 66.4% male) was re-examined to assesses the unique and combined associations of self-, personal, and workplace stigma with workers’ compensation claims experiences and recovery. Participants responded to self-report stigma items (predictor variables), perceived stress, fairness, and support perceptions of going through the claims process and its impact on recovery (outcome variables). Multiple regression analyses revealed that the combined stigma dimensions predicted about one fifth of the variance of claims and recovery perceptions. Organisational commitment beliefs and the self-stigma dimension of experiences with others were the two most important, albeit weak, unique predictors across outcomes. Given the small but consistent influences of organisational commitment beliefs and the self-stigma dimension of experiences with others, it seems warranted to apply workplace interventions that are looking to establish positive workplace contact and a supportive organisational culture to alleviate negative effects attributable to mental health stigma.
Spatial and Temporal Distribution and the Driving Factors of Carbon Emissions from Urban Production Energy Consumption
Authors: Fu Liyuan , Wang Qing
Published: September 2022

Urban production energy consumption produces a large amount of carbon emissions, which is an important source of global warming. This study measures the quantity and intensity of carbon emissions in 30 provinces of China based on urban production energy consumption from 2005–2019, and uses the Dagum Gini coefficient, kernel density estimation, carbon emission classification and spatial econometric model to analyze the spatial and temporal distribution and driving factors of quantity and intensity of carbon emissions from China and regional production energy consumption. It was found that the growth rate of carbon emission quantity and carbon emission intensity of production energy consumption decreased year by year in each province during the study period. The imbalance of carbon emission was strong, with different degrees of increase and decrease, and there were big differences between eastern and western regions. The classification of carbon emissions differed among provinces and there was heterogeneity among regions. The quantity and intensity of carbon emissions of production energy consumption qwre affected by multiple factors, such as industrial structure. This study provides an in-depth comparison of the spatial and temporal distribution and driving factors of quantity and intensity of carbon emissions of production energy consumption across the country and regions, and provides targeted policies for carbon emission reduction across the country and regions, so as to help achieve China’s “double carbon” target quickly and effectively.
A Potential Driver of Disseminated Intravascular Coagulation in Heat Stroke Mice: Neutrophil Extracellular Traps
Authors: Zhang Yuling , Deng Xiling , Zhang Jing , Zhang Liang , Akram Zubair , Zhang Bo , Sun Shiguo
Published: September 2022

Aims: Disseminated intravascular coagulation (DIC) is a common complication of heat stroke (HS) patients, and it is one of the important reasons leading to multiple organ failure and even death. The association between neutrophil extracellular traps (NETs) and DIC is unclear in HS mice. Methods and results: Here, HS was induced by the combination of hyperthermia (HT) and lipopolysaccharide (LPS). The DIC was evaluated by measuring prothrombin time (PT), D-dimer, thrombomodulin (TM), fibrinogen (FIB), and platelet (PLT). The expression of citrullinated-histone (CitH3) was analyzed by Western blotting. The formation of NETs was observed by immunofluorescence microscopy. The risk of HS-induced DIC was increased when HT was combined with LPS. The markers of NETs were significantly higher than those in the control group, and the NETs derived from HS promoted the development of DIC. DNase I improved coagulation dysfunction via the clearance of NETs caused by neutrophil aggregation. Conclusions: Degradation of NETs reduced the risk of developing DIC, and thus the survival rate of mice was improved. These results indicate that NETs may hold potential alternative therapeutic strategies for the treatment of DIC in HS patients.
RecMin Variograms: Visualisation and Three-Dimensional Calculation of Variograms in Block Modelling Applications in Geology and Mining
Authors: Buelga Díaz Arturo , Castañón Fernández César , Ares Gonzalo , Prieto Daniel Arias , Álvarez Isidro Diego
Published: September 2022

Variogram calculation is a fundamental tool for studying ore grade data in mineral deposits. It allows the discovery of hidden structures within the data and preferential directions of mineralization where the geological continuity is longer. The variogram allows us to classify samples and to define both the search radii for interpolation and the use of kriging as an interpolation and resource classification method. It is not difficult to use it in one dimension; complexity increases in two dimensions as the necessity to search for possible grade anisotropies in all directions arises. Three-dimensionally it is even more difficult to try to define the parameters of lag, bandwidth, and tolerances that define the data of the variogram calculation algorithm. There are rules of thumb to help in the development, but a trial-and-error approach is used in order to find enough sample pairs to allow the generation of variograms truly representative of the data. In this paper, two strategies are shown to help in variogram construction, the use of variogram maps and the graphical representation of the pair search areas (cones or pencils). The freeware tool RecMin Variograms has been developed and is freely available for download at its website; it is easy to learn and use. R code based on RGeostats libraries is used to check the operation and results of RecMin Variograms. Applying geostatistics to mineral deposits is essential to know the areas with the highest grades, thus allowing exact planning of future mining exploitation and minimizing mine footprint and environmental impact.
A New Structural Model of Apolipoprotein B100 Based on Computational Modeling and Cross Linking
Authors: Jeiran Kianoush , Gordon Scott M. , Sviridov Denis O. , Aponte Angel M. , Haymond Amanda , Piszczek Grzegorz , Lucero Diego , Neufeld Edward B. , Vaisman Iosif I. , Liotta Lance , Baranova Ancha , Remaley Alan T.
Published: September 2022

ApoB-100 is a member of a large lipid transfer protein superfamily and is one of the main apolipoproteins found on low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) particles. Despite its clinical significance for the development of cardiovascular disease, there is limited information on apoB-100 structure. We have developed a novel method based on the “divide and conquer” algorithm, using PSIPRED software, by dividing apoB-100 into five subunits and 11 domains. Models of each domain were prepared using I-TASSER, DEMO, RoseTTAFold, Phyre2, and MODELLER. Subsequently, we used disuccinimidyl sulfoxide (DSSO), a new mass spectrometry cleavable cross-linker, and the known position of disulfide bonds to experimentally validate each model. We obtained 65 unique DSSO cross-links, of which 87.5% were within a 26 Å threshold in the final model. We also evaluated the positions of cysteine residues involved in the eight known disulfide bonds in apoB-100, and each pair was measured within the expected 5.6 Å constraint. Finally, multiple domains were combined by applying constraints based on detected long-range DSSO cross-links to generate five subunits, which were subsequently merged to achieve an uninterrupted architecture for apoB-100 around a lipoprotein particle. Moreover, the dynamics of apoB-100 during particle size transitions was examined by comparing VLDL and LDL computational models and using experimental cross-linking data. In addition, the proposed model of receptor ligand binding of apoB-100 provides new insights into some of its functions.
α-Gal Nanoparticles Mediated Homing of Endogenous Stem Cells for Repair and Regeneration of External and Internal Injuries by Localized Complement Activation and Macrophage Recruitment
Authors: Galili Uri , Goldufsky Josef W. , Schaer Gary L.
Published: September 2022

This review discusses a novel experimental approach for the regeneration of original tissue structure by recruitment of endogenous stem-cells to injured sites following administration of α-gal nanoparticles, which harness the natural anti-Gal antibody. Anti-Gal is produced in large amounts in all humans, and it binds the multiple α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) presented on α-gal nanoparticles. In situ binding of anti-Gal to α-gal nanoparticles activates the complement system and generates complement cleavage chemotactic-peptides that rapidly recruit macrophages. Macrophages reaching anti-Gal coated α-gal nanoparticles bind them via Fc/Fc receptor interaction and polarize into M2 pro-reparative macrophages. These macrophages secrete various cytokines that orchestrate regeneration of the injured tissue, including VEGF inducing neo-vascularization and cytokines directing homing of stem-cells to injury sites. Homing of stem-cells is also directed by interaction of complement cleavage peptides with their corresponding receptors on the stem-cells. Application of α-gal nanoparticles to skin wounds of anti-Gal producing mice results in decrease in healing time by half. Furthermore, α-gal nanoparticles treated wounds restore the normal structure of the injured skin without fibrosis or scar formation. Similarly, in a mouse model of occlusion/reperfusion myocardial-infarction, near complete regeneration after intramyocardial injection of α-gal nanoparticles was demonstrated, whereas hearts injected with saline display ~20% fibrosis and scar formation of the left ventricular wall. It is suggested that recruitment of stem-cells following anti-Gal/α-gal nanoparticles interaction in injured tissues may result in induction of localized regeneration facilitated by conducive microenvironments generated by pro-reparative macrophage secretions and “cues” provided by the extracellular matrix in the injury site.
A Survey on Computational Methods for Investigation on ncRNA-Disease Association through the Mode of Action Perspective
Authors: Bang Dongmin , Gu Jeonghyeon , Park Joonhyeong , Jeong Dabin , Koo Bonil , Yi Jungseob , Shin Jihye , Jung Inuk , Kim Sun , Lee Sunho
Published: September 2022

Molecular and sequencing technologies have been successfully used in decoding biological mechanisms of various diseases. As revealed by many novel discoveries, the role of non-coding RNAs (ncRNAs) in understanding disease mechanisms is becoming increasingly important. Since ncRNAs primarily act as regulators of transcription, associating ncRNAs with diseases involves multiple inference steps. Leveraging the fast-accumulating high-throughput screening results, a number of computational models predicting ncRNA-disease associations have been developed. These tools suggest novel disease-related biomarkers or therapeutic targetable ncRNAs, contributing to the realization of precision medicine. In this survey, we first introduce the biological roles of different ncRNAs and summarize the databases containing ncRNA-disease associations. Then, we suggest a new trend in recent computational prediction of ncRNA-disease association, which is the mode of action (MoA) network perspective. This perspective includes integrating ncRNAs with mRNA, pathway and phenotype information. In the next section, we describe computational methodologies widely used in this research domain. Existing computational studies are then summarized in terms of their coverage of the MoA network. Lastly, we discuss the potential applications and future roles of the MoA network in terms of integrating biological mechanisms for ncRNA-disease associations.
Complementary Sets of Autoantibodies Induced by SARS-CoV-2, Adenovirus and Bacterial Antigens Cross-React with Human Blood Protein Antigens in COVID-19 Coagulopathies
Authors: Root-Bernstein Robert , Huber Jack , Ziehl Alison
Published: September 2022

COVID-19 patients often develop coagulopathies including microclotting, thrombotic strokes or thrombocytopenia. Autoantibodies are present against blood-related proteins including cardiolipin (CL), serum albumin (SA), platelet factor 4 (PF4), beta 2 glycoprotein 1 (β2GPI), phosphodiesterases (PDE), and coagulation factors such as Factor II, IX, X and von Willebrand factor (vWF). Different combinations of autoantibodies associate with different coagulopathies. Previous research revealed similarities between proteins with blood clotting functions and SARS-CoV-2 proteins, adenovirus, and bacterial proteins associated with moderate-to-severe COVID-19 infections. This study investigated whether polyclonal antibodies (mainly goat and rabbit) against these viruses and bacteria recognize human blood-related proteins. Antibodies against SARS-CoV-2 and adenovirus recognized vWF, PDE and PF4 and SARS-CoV-2 antibodies also recognized additional antigens. Most bacterial antibodies tested (group A streptococci [GAS], staphylococci, Escherichia coli [E. coli], Klebsiella pneumoniae, Clostridia, and Mycobacterium tuberculosis) cross-reacted with CL and PF4. while GAS antibodies also bound to F2, Factor VIII, Factor IX, and vWF, and E. coli antibodies to PDE. All cross-reactive interactions involved antibody-antigen binding constants smaller than 100 nM. Since most COVID-19 coagulopathy patients display autoantibodies against vWF, PDE and PF4 along with CL, combinations of viral and bacterial infections appear to be necessary to initiate their autoimmune coagulopathies.