bluuebunny/crossref_metadata_2025 huggingface dataset:
The interleukin-enhanced binding factor 3-mediated inhibition of nitric oxide production via phosphoinositide 3-kinase/protein kinase B pathway contributes to central cardiovascular regulation in the rostral ventrolateral medulla in hypertension
Authors: Tan Xing , Ye Peng , Li Wan-Yang , Gao Yuan , Leng Yue-Qi , Duan Wei , Wang Yang-Kai , Wang Wei-Zhong
Published: October 2022

Hypertension is characterized by sympathetic hyperactivity, which is related to the overexcitation of the presympathetic neurons in the rostral ventrolateral medulla (RVLM). Nitric oxide (NO) has been reported to be a vital neuromodulator involved in central cardiovascular regulation. However, the mechanism of interleukin-enhanced binding factor 3 (ILF3) participating in blood pressure (BP) regulation is still unclear. Therefore, this study aims to clarify the role of ILF3 within the rostral ventrolateral medulla (RVLM) in regulating NO in hypertension. It was found that the expression level of ILF3 was significantly increased in the RVLM of spontaneously hypertensive rats (SHR) compared with Wistar-Kyoto (WKY) rats through microarray gene expression analysis, Western blot, and immunofluorescence. Overexpression of ILF3 by injecting constructed adenovirus into the RVLM increased the BP and renal sympathetic nerve activity (RSNA) of the WKY rats, significantly decreasing NO production and neuronal nitric oxide synthase (nNOS) expression. Knockdown of ILF3 in the RVLM of SHR significantly reduced BP but increased NO production and the neuronal nitric oxide synthase (nNOS) expression. Furthermore, it was found that the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway was activated via Western blotting in the RVLM after overexpression of ILF3, whereas it was attenuated after knockdown of ILF3 in SHR. In addition, inhibition of PI3K by intracisternal infusion of the PI-103 attenuated the increase in Akt phosphorylation and decrease in nNOS expression and NO production caused by overexpressing ILF3, which ultimately blunted high BP induced by overexpressing ILF3. Taken together, this current study suggests that ILF3 participates in high BP via reducing NO production in the RVLM through PI3K/Akt pathway.
A 10-mg dose of amiloride increases time to failure during blood-flow-restricted plantar flexion in healthy adults without influencing blood pressure
Authors: Stavres Jon , Luck J. Carter , Hamaoka Takuto , Blaha Cheryl , Cauffman Aimee , Dalton Paul C. , Herr Michael D. , Ruiz-Velasco Victor , Carr Zyad J. , Janicki Piotr , Cui Jian
Published: October 2022

Amiloride has been shown to inhibit acid-sensing ion channels (ASICs), which contribute to ischemia-related muscle pain during exercise. The purpose of this study was to determine if a single oral dose of amiloride would improve exercise tolerance and attenuate blood pressure during blood-flow-restricted (BFR) exercise in healthy adults. Ten subjects (4 females) performed isometric plantar flexion exercise with BFR (30% maximal voluntary contraction) after ingesting either a 10-mg dose of amiloride or a volume-matched placebo (random order). Time to failure, time-tension index (TTI), and perceived pain (visual analog scale) were compared between the amiloride and placebo trials. Mean blood pressure, heart rate, blood pressure index (BPI), and BPI normalized to TTI (BPInorm) were also compared between trials using both time-matched (TM50 and TM100) and effort-matched (T50 and T100) comparisons. Time to failure (+69.4 ± 63.2 s, P < 0.01) and TTI (+1,441 ± 633 kg·s, P = 0.02) were both significantly increased in the amiloride trial compared with placebo, despite no increase in pain (+0.4 ± 1.7 cm, P = 0.46). In contrast, amiloride had no significant influence on the mean blood pressure or heart rate responses, nor were there any significant differences in BPI or BPInorm between trials when matched for time (all P ≥ 0.13). When matched for effort, BPI was significantly greater in the amiloride trial (+5,300 ± 1,798 mmHg·s, P = 0.01), likely owing to an increase in total exercise duration. In conclusion, a 10-mg oral dose of amiloride appears to significantly improve the tolerance to BFR exercise in healthy adults without influencing blood pressure responses.
Observation on the Clinical Efficacy of Traditional Chinese Medicine Non-Drug Therapy in the Treatment of Insomnia: A Systematic Review and Meta-Analysis Based on Computer Artificial Intelligence System
Authors: Zhuang Jingqing , Wu Jian , Fan Liang , Liang Chongnan
Published: October 2022

Objective. Insomnia is a common and frequently occurring disease affecting the health of the population, which can seriously affect the work and life of patients. Drug treatment of insomnia has a rapid onset of action but has a large adverse reaction incidence rate. Traditional external treatment of traditional Chinese medicine (TCM) belongs to a type of non-drug therapy, the treatment of insomnia has a long history, but the methods of non-drug treatment of TCM are diverse, and the efficacy is also different. This study investigated the efficacy of TCM non-drug therapy in the treatment of insomnia by means of literature search and meta-analysis. Methods. We searched Embase, Pubmed, OVid, WOS, CNKI, and CBM for randomized controlled trials (RCTs) on the use of TCM as a non-drug treatment for primary insomnia. After doing a literature search according to the inclusion and exclusion criteria, we used Cochrane rob v2.0 to assess the potential for bias in the studies that were included, and we did a combined analysis and assessment of the effectiveness of the therapy. Results. 16 articles were included in this study for quantitative analysis, and a total of 1285 patients participated in the study, including 643 patients in the intervention group and 642 patients in the control group. Meta-analysis showed that non-drug therapy of TCM could improve the treatment response rate of insomnia patients [OR = 6.88, 95%CI (4.40,10.74), Z = 8.48,

P
<
0.0001

], reduce post-treatment PSQI total score [MD = −3.42, 95%CI (−4.62, −2.22), Z = −5.60,

P
<
0.0001

], and improved patient anxiety [SMD = −1.25, 95%CI (−2.13, −0.37), Z = −2.78,

P
=
0.01

] and degree of depression [SMD = −1.53, 95%CI (−2.84, −0.21), Z = −2.28,

P
=
0.02

]. The heterogeneity survey showed that treatment time was one of the sources of heterogeneity. Meta-regression analysis revealed that publication year, patient age, sample size, and intervention characteristics were not specific factors affecting the combined results. Discussion. TCM non-drug therapy (acupuncture, moxibustion, massage, and auricular point pressing beans) can significantly improve the PSQI score of patients after treatment and improve the degree of anxiety and depression of patients, with significant effect, which is worthy of clinical promotion.
Experiment Analysis of Drilling Feedback Signal from Simulation of Roadway Roof
Authors: Guo Gang , Zhao Haoyuan , Qi Zhilong , Hu Chenjun , Zhao Baofu , Wang Shengzhi
Published: October 2022

To simulate the condition of unstable rock formation in the stable rock formation of the roadway roof, the complete and broken rock formations on the roadway roof were simulated by superimposing and combining complete specimens and broken specimens, hence providing an appropriate way to obtain the drilling vibration signals to find regular patterns which can be used to judge the state of the rock layers. The Fourier transform analysis method and the wavelet transform analysis method are, respectively, used for the vibration signals to extract the eigenvalues of the vibration signals that are quite different in the complete layer and broken layers. Effective values, kurtosis factor, pulse factors, and gravity amplitude were used to construct the eigenvectors, which were most suitable as the basis for judging the integrity of rock formation after analysis, providing an effective way to deal with drilling signal. The wavelet threshold denoising could adaptively reduce the noise of the signals, and the unbiased likelihood estimation threshold rule had the best denoising effect. Well, through the extraction and comparison of the wavelet feature-scale entropy values, it is concluded that the wavelet analysis can be used as a reference rather than a decisive factor to determine the complete and broken layers. And more research is needed to determine the selection method of wavelet.
LGBM: An Intrusion Detection Scheme for Resource-Constrained End Devices in Internet of Things
Authors: Cong Yong-Quan , Guan Ting , Cui Ju-Fu , Cheng Xiang-Guo
Published: October 2022

The intrusion detection schemes (IDSs) based on the Gradient Boosting Decision Tree (GBDT) face three problems: unbalanced training data distribution, large dimensionality of data features, and difficulty in model parameter optimization, which lead to weak monitoring capability and high false positive rate. For the problem of unbalanced training data distribution, we make the one-sided gradient oversampling algorithm to ensure the balance between the data of each category. To tackle the problem of the large dimensionality of data features, we develop a hierarchical cross-validation algorithm for binding mutually exclusive features. To address the problem of difficulty in model parameter optimization, we design a Bayesian optimization algorithm to make the model parameter search process more targeted and reduce the model training cost by establishing functional relationships between hyperparameters and target functions. The detailed experimental results show that the scheme can effectively solve the problems of data imbalance, high-dimensional data features, and low parameter finding efficiency, and improve the model’s ability to monitor the attack behavior.
A Wideband Antenna for GNSS Applications with Improved Orthogonal Dipole
Authors: Daming Lin , Encheng Wang , Jie Wang
Published: October 2022

This letter presents a wideband antenna for high-precision Global Navigation Satellite System (GNSS) applications. The antenna consists of a pair of improved orthogonal dipoles that are fed by a feed network with Composite Right/Left-Handed Transmission Lines (CRLH-TLs), a polygonal patch, and a reflector. By using improved orthogonal dipole, the beamwidth of the antenna is broadened. The CRLH-TLs is adopted in feed network to obtain outputs with stable phase difference. Experiment results indicate that the antenna has a less than 10-dB return-loss bandwidth in the range of 1.1 to 1.7 GHz, a 3-dB axial-ratio bandwidth from 1.2 to 1.6 GHz, and a larger than 6.37 dBi gain in the whole operating band. The measured results show that the proposed antenna has stable performance in the whole operating band, which means that it is a suitable antenna used for GNSS applications.
Analysis of the Influence of Network Informatization Construction on Rural Economic Development
Authors: Hu MingQi , Zhang ZuDi
Published: October 2022

At present, the pace of network information construction in China is accelerating. Foshan has constantly explored the construction road with Foshan characteristics. Many aspects of network information construction have reached a relatively high level in China, but compared with the coastal or foreign developed areas, the level of Foshan is far lower. With the steady and progressive development and construction of urban and rural overall planning, there has been a situation that “the rural area starts but the climax is in the city.” The relatively backward rural economy has become a stumbling block to the development of Foshan City. Taking Foshan as an example, this study expounds on the current situation of impact analysis and research at home and abroad, understands the connotation of network information construction, analyzes the current situation of rural economic development under the background of network information construction in Foshan, finds out the factors restricting the development of rural economy in Foshan, and puts forward relevant countermeasures and suggestions to reduce the impact of these factors and promote the development of rural economy in Foshan.
The Benefit of Optimal Dietary Lipid Level for Black Seabream Acanthopagrus schlegelii Juveniles under Low-Salinity Environment
Authors: Jin Min , Li Xuejiao , Shen Yuedong , Bao Yangguang , Yang Bingqian , Wu Zhaoxun , Jiao Lefei , Zhou Qicun
Published: October 2022

The present study was aimed at evaluating the regulatory effects of dietary lipid levels on growth performance, osmoregulation, fatty acid composition, lipid metabolism, and physiological response in Acanthopagrus schlegelii under low salinity (5 psu). An 8-week feeding trial was conducted in juvenile A. schlegelii with an initial weight of

2.27
±
0.05

 g, and six isonitrogenous experimental diets were formulated with graded levels of lipid: 68.7 g/kg (D1), 111.7 g/kg (D2), 143.5 g/kg (D3), 188.9 g/kg (D4), 239.3 g/kg (D5), and 269.4 g/kg (D6), respectively. Results indicated that fish fed with diet containing 188.9 g/kg lipid significantly improved growth performance. Dietary D4 improved ion reabsorption and osmoregulation by increasing the concentrations of Na+, K+, and cortisol in serum and activities of Na+/K+-ATPase as well as expression levels of osmoregulation related to gene expression levels in the gill and intestine. The expression levels of long chain polyunsaturated fatty acid biosynthesis-related genes were dramatically upregulated when dietary lipid levels increased from 68.7 g/kg to 189.9 g/kg with levels of docosahexaenoic (DHA), eicosapentaenoic (EPA), and DHA/EPA ratio being highest in the D4 group. When fish fed dietary lipid levels from 68.7 g/kg to 188.9 g/kg, lipid homeostasis could be maintained by upregulating sirt1 and pparα expression levels, whereas lipid accumulation was observed in dietary lipid levels of 239.3 g/kg and over. Fish fed with high dietary lipid levels resulted in physiological stress related to oxidative stress and endoplasmic reticulum stress. In conclusion, based on weight gain, the optimal dietary lipid requirement of juvenile A. schlegelii reared at low-salinity water is 196.0 g/kg. These findings indicate that the optimal dietary lipid level can improve growth performance, n-3 LC-PUFA accumulation, and osmoregulatory ability and maintain lipid homeostasis and normal physiological functions of juvenile A. schlegelii.
Plasma Cell Gingivitis Treated with Photobiomodulation, with No Recurrence for a Five-Year Follow-Up
Authors: Pulicari Federica , Pellegrini Matteo , Pascadopoli Maurizio , Porrini Massimo , Kuhn Elisabetta , Scribante Andrea , Spadari Francesco
Published: October 2022

Introduction. Plasma cell gingivitis (PCG) is a chronic inflammatory disease usually affecting the vestibular portion of the gingival mucosa. Clinical presentation is marked by erythematous macules of intense red color, confluent, and delimited from the healthy neighboring mucosa. Generally asymptomatic, the gum lesions sometimes are accompanied by burning sensations and a sense of local tension. Recommended treatment is the use of topical steroids, but with apparent initial healing that is not stable over time. The present case report concerns a patient diagnosed with PCG in November 2017, with a five-year follow-up. This is the first patient with PCG successfully treated with non-surgical periodontal therapies associated with photobiomodulation (PBM). Methods. A 64-year-old male patient had intense erythema and edema on the vestibular side of the gingival mucosa area from 1.5 to 2.5. The patient’s symptomatic subjectivity parameters were evaluated through dedicated questionnaires. Erythema and gingival bleeding were also evaluated. Periodontal charting was not pathological, but intense bleeding was noted. Multiple biopsies were performed, and microscopic findings confirmed the clinical hypothesis of PCG. Results. The treatment applied was PBM associated with periodontal therapy. The patient demonstrated a progressive improvement in clinical parameters considered and reported symptoms. During the five-year follow-up, no recurrence of the disease was observed. Conclusions. The combined PBM and periodontal therapies have proved to be sufficiently effective in the control of PCG, showing reduction of the intense inflammatory, erythematous component, and gingival bleeding, and are a valid treatment alternative to topical steroids.
[Retracted] Sustainable Trend of Big Data in Enterprise Supply Chain Under the Artificial Intelligence Green Financial System
Authors: Wu He , Zhang Xuancheng , Wang Yiqin
Published: October 2022

Today, with the rapid development of the Internet, society has entered the era of “information explosion.” Financial data are a particularly important part of network information, and it has also reached a new level of public demand. The frequent appearance of words such as “carbon peak” and “green” indicates the transition of national policies to the field of sustainable development. Sustainable development would become an inevitable choice, and a green supply chain has become a new trend under this policy background. Supply chain finance uses the ideas and methods of key supply management to provide financial services to related enterprises. If an enterprise cannot acquire, organize, and use the information and data in the supply chain, it is likely to be outdated or even abandoned in the short term. This paper takes the artificial intelligence green financial system as the background and uses the cooperation theory model to analyze and predict the big data information of the enterprise supply chain. It realizes the transformation of information into sustainable resources for enterprises and releases the huge potential of big data. In this model, this model not only helped the company’s overall profit increase by about 8.79% but also provided scientific support for corporate decision‐making and promoted the development of the company.
Construction of Mobile Internet Financial Risk Cautioning Framework Based on BP Neural Network
Authors: Zang Wu
Published: October 2022

With the emergence of the 21st-century global economy, the international financial system faces economic risks. A competitive cautioning model for financial management is required to mitigate risks and losses in the financial sector. The financial losses of the banking industry have been categorized and analyzed using the Internet of Things (IoT) and big data technologies to minimize the economic risk of commercial banks in mobile internet finance (MIF). This article proposes a new financial risk cautioning framework (FRCF) based on the IoT, big data, and back propagation-neural network (BP-NN) to ensure steady growth of MIF in the long term. In this article, a big data technology-based approach for data recognition and mining has been suggested. A BP-NN-based method for risk identification and assessment in MIF is also presented. The BP-NN technique calculates each neural network (NN) layer’s node count, transfer functions, learning rate, and other characteristics. The proposed FRCF has been developed through the proper construction, analysis, and testing of many information samples. A conceptual understanding of the use of IoT, big data, and artificial intelligence (AI) technologies through NN models in the financial industry has been described in the article. The proposed FRCF can predict the MIF risks associated with the MIF lending infrastructure with a 98.2% accuracy.
Trends of Malaria Prevalence in Selected Districts of Kaffa Zone, Southwest Ethiopia
Authors: Duguma Tadesse , Tekalign Eyob , Abera Mitiku
Published: October 2022

Background. Malaria remains one of the world's major public health issues, particularly in low- and middle-income countries. In Ethiopia, cases have declined over the last decade, and attempts to eradicate the illness are underway. The purpose of this study was to determine trends in malaria prevalence in selected areas of the Kaffa zone during the last five years (Decha and Gimbo districts). Methods. All malaria cases registered from 2017 to 2021 were reviewed to assess the trends of malaria prevalence. A checklist was used to collect the secondary data from registers and entered it into an Excel spreadsheet, which was then exported to the Statistical Package for the Social Sciences (SPSS) version 25.0 for analysis. The malaria incidence rate was calculated with the total number of person-years as the denominator and the number of new cases as the numerator. Seasons, years, gender, age, and malaria parasite species were all used to show trends in malaria transmission in the areas. Tables and figures were used to present the data. Results. Out of 20,045 individuals screened for malaria, 13.6% (2,732/20,045) of them were recorded to have Plasmodium species tested using microscopy and rapid diagnostic test (RDT). Plasmodium falciparum, Plasmodium vivax, and mixed infections (Plasmodium falciparum + Plasmodium vivax) accounted for 1200 (5.9%), 1114 (5.56%), and 418 (2.09%) of the confirmed malaria cases, respectively. Overall, malaria incidence decreased with an interannual variation, from 4.08 cases per 1000 person-years in 2017 to 3.62 cases per 1000 person-years in 2018, then increased to 4.94 cases per 1000 person-years in 2021. Conclusion. The malaria trend over the last five years has revealed a nonconsistent pattern of cases in different years. The number of malaria cases has shown an increase over the period of 2017 to 2021. Interannual and intra-annual variations have been observed in the transmission of the disease.
Interactive Display of Images in Digital Exhibition Halls under Artificial Intelligence and Mixed Reality Technology
Authors: Liu Xu , Zhang Nan
Published: October 2022

The attractiveness of traditional exhibition halls to young people is gradually decreasing. Combining modern digital technology to improve the display effect of the exhibition hall can effectively enhance the effect of cultural publicity. This article introduces the technology of image interaction and mixed reality (MR) to improve the historical and cultural propaganda level of the Shaanxi exhibition hall. The advantages of MR technology in applying digital exhibition halls are theoretically expounded. A theoretical plan for Shaanxi history and culture-related display areas is designed using artificial intelligence combined with  MR  technology. In addition, the survey respondent’s evaluation of the effect of the new exhibition hall is obtained using a questionnaire survey. The survey results show that 97% of people like the history and culture of Shaanxi but only 13% of the people say they know or know very well about the history and culture of Shaanxi. In addition, 60% of the tourists say they are satisfied with the cultural experience of Shaanxi, and only 27% of the tourists are very satisfied. Also, 96% of tourists are willing to experience Shaanxi’s history and culture through digital exhibition halls, and 93% are willing to participate in cultural experience activities based on MR technology. The survey results prove that tourists are satisfied with the effect of the new exhibition hall. Tourists want to add a distinctive form of cultural experience to the exhibition hall. They are willing to accept digital exhibition halls incorporating MR technology and are very happy to participate in the exhibition method of image interaction. This shows that the use of image interactive display based on MR technology in the layout of the exhibition hall is recognized by people and has strong feasibility. This article has reference significance for the digital upgrade of the exhibition hall and the development of the cultural tourism industry.
The Value of Macrogene Second-Generation Sequencing in the Diagnosis, Guidance of Drug Use, and Efficacy Monitoring of Infectious Pneumonia in Premature Infants
Authors: Wang LiLi , Zha Ping , Wang YuJuan , Kong Ying , Su Yu , Dai LiYing , Wang Yang
Published: October 2022

Objective. A group-controlled trial was conducted to explore the value of macrogene second-generation sequencing in the diagnosis, drug use, and efficacy monitoring of infectious pneumonia in premature infants. Methods. One hundred and thirty-eight premature infants with suspected infectious pneumonia treated in our hospital from March 2019 to June 2022 were selected as subjects. All patients underwent deep phlegm extraction and were randomly divided into two groups. 69 cases of control group were treated with general bacterial and fungal culture. The lavage fluid of the remaining 69 cases of observation group were detected by metagenomic next-generation sequencing (mNGS). The number of diagnosed preterm infants with infectious pneumonia was compared between the two groups, and the diagnostic value of the two methods was analyzed by the receiver operator characteristic (ROC) curve. Then, the differences in clinical efficacy, antimicrobial neonatal intensive care unit (NICU) use time, antimicrobial adjustment frequency, NICU stay time, hospital stay, and serum inflammatory factors were compared between the two groups. Results. The positive rate of mNGS pathogen detection in the lavage fluid of the observation group was 92.75% (64/69). The positive rate of the culture of the lavage fluid of the control group was 52.17% (36/69). The ROC curve analysis showed that the ROC AUC of traditional culture was 0.752 (

95
%
CI
=
0.610

-0.894), and that of mNCS was 0.934 (

95
%
CI
=
0.854

-0.999). In the observation group, there were 35 cases of bacterial infection, 20 cases of fungi, 4 cases of virus, and 5 cases of Chlamydia psittaci. In the control group, 26 cases of bacterial infection and 9 cases of fungi were detected; but viruses and other mycoplasmas could not be detected. After 2 weeks of treatment, the effective rate of the observation group was 95.31%, while that of the control group was 69.44%. The NICU use time, adjustment frequency, NICU stay time, and hospitalization time of antibiotics in the observation group were significantly less than those in the control group, and the difference was statistically significant (

P
<
0.05

). After treatment, the levels of serum interleukin-6 (IL-6), procalcitonin (PCT), and hypersensitivity-C-reactive protein (hs-CRP) in observation group were significantly higher than those in control group, and the difference was statistically significant (

P
<
0.05

). Conclusion. mNGS can improve the efficiency of clinical diagnosis of infectious pneumonia in premature infants, effectively improve the detection rate of pathogens and the clinical efficacy of premature infants. At the same time, it can also assist the clinical efficacy monitoring and adjust the treatment plan at any time.
A Heart Failure Model Established by Pressure Overload Caused by Abdominal Aortic Contraction in Rat
Authors: Dai Wang Sheng , Chen Yu-Kun , Lin Shi-Hao , Chen Qiang , Cao Hua
Published: October 2022

Heart failure is a complex clinical syndrome in which ventricular filling or ejection capacity is impaired due to structural or functional diseases of the heart. In order to establish a stable heart failure model, we investigated cardiac parameters in rats with abdominal aortic contraction and normal rats, including the left ventricular posterior wall diameter (LVPWd), the interventricular septum thickness of end-diastolic (IVSd), the left ventricular end-diastolic diameter (LVEDd), the left ventricular ejection fraction (LVEF), and left ventricular fractional shortening (LVFS). Rats were randomly divided into experimental group (

n
=
20

) and control group (

n
=
20

). The experimental group underwent modified abdominal aortic constriction, while the control group only isolated the abdominal aorta without constriction. The results showed that the survival rate of rats in the experimental group was 85% after one week of operation, while the survival rate of rats in the control group was 100%. Five weeks after operation, the left ventricular posterior wall diameter (LVPWd) and the interventricular septum thickness of end-diastolic (IVSd) in the experimental group were all increased compared with those in the control group, and the differences were statistically significant (

p
<
0.05

); the left ventricular end-diastolic diameter (LVEDd) in the experimental group showed an increasing trend compared with the control group, but

p
>
0.05

; compared with the control group, the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) in the experimental group showed downward trend, but

p
>
0.05

. 10 weeks after operation, the LVPWd, IVSd, and LVEDd of the experimental group were increased compared with the control group,

p
<
0.05

, and the LVEF and LVFS of the experimental group were decreased compared with the control group,

p
<
0.05

. Compared with the control group, the BNP of the experimental group increased significantly,

p
<
0.05

. The heart weight index and left ventricular weight index of rats in the experimental group were significantly higher than those in the control group,

p
<
0.05

. HE staining showed that the myocardial cells in the experimental group increased in volume, disordered cell arrangement, widened gaps, increased nuclear hyperchromia, and uneven staining. This paper provides a theoretical basis for the study of heart failure.
Integrated Urban Design Methodology of “Planning-Construction-Management” and Intelligent Management and Control Based on Teaching Reproduction Robot
Authors: Wang Haipeng
Published: October 2022

With regard to the problems in the integrated urban construction based on “planning-construction–management,” the main issues that include the tight construction cycle, the high operation standards, the relatively extensive range of management data sources, the uneven information control, the relatively significant difficulty of management and control, and so on. In this paper, an integrated urban system of “planning-construction-management” based on the management level of the construction projects is established mainly through the application of the teaching reproduction robot (TRR), the engineering information, management and control procedures, and the system framework and performance. Among them, the management and control cover the design control, the quality supervision, the schedule control module, the safety control, the video monitoring, and the facility movement control. The digital technology is included to achieve an integrated management and control model for the engineering design, construction, and operation in its practical operational form. The introduction of the management and control described above has led to relatively good results for the project by operating on the road project with a public-private partnership (PPP) in the startup area of the Chengdu Tianfu International Airport New Town.
Nutritional and Phytochemical Variation of Marula (Sclerocarya birrea) (Subspecies caffra and birrea) Fruit among Nine International Provenances Tested in Malawi
Authors: Kamanula Mussa , Munthali Chimuleke Yagontha , Kamanula John Finias
Published: October 2022

Sclerocarya birrea (Marula) is one of the indigenous fruit trees that was selected for domestication in Malawi. This study was conducted to assess nutritional and phytochemical variation of Sclerocarya birrea (subspecies caffra and birrea) fruits planted in an international provenance trial in Malawi. Vitamin C, calcium, iron, zinc, fat, and fibre content ranged from 6 to 81 mg/100 g; 1.8 to 5.3 mg/100 g; 1.4 to 3.3 mg/100 g; 0.3 to 0.8 mg/100 g; 51.6 to 57.7%; and 4.1 to 6.9%, respectively. Phytochemical scores showed that all nine provenances contained weak (+) concentration of alkaloids. Kalimbeza (Namibia) and Magamba-Turiani (Tanzania) provenances showed moderate (++) concentrations of saponins. Chikhwawa (Malawi), Missira (Mali), and Moamba (Mozambique) provenances had moderate (++) concentrations of tannins. Missira (Mali) and Kalimbeza (Namibia) provenances showed weak (+) concentration of terpenoids. Variations observed in nutritional and phytochemical composition could be attributed to genetic make-up and origin of the genotypes, since all genotypes were planted in the same environment. Therefore, selection of Sclerocarya birrea for domestication purposes should consider the provenance (origin of genotypes) and subspecies levels. Further studies should investigate vegetative propagation and heritability of nutritional and phytochemical traits before the use of seed for propagation.
Influence of Ginsenoside Rh2 on Cardiomyocyte Pyroptosis in Rats with Acute Myocardial Infarction
Authors: Song Wanmei , Dai Bin , Dai Yuntang
Published: October 2022

Objective. This paper intends to verify through in vivo experiments whether ginsenoside Rh2 (G-Rh2) can play an anti-inflammatory role by modulating cardiomyocyte (CM) pyroptosis in rats with acute myocardial infarction (AMI), thereby alleviating myocardial injury. Methods. Twenty SD rats were randomized into control, L-Rh2, M-Rh2, and H-Rh2 groups, among which the latter three groups were modeled for AMI and given an intraperitoneal injection of G-Rh2 (L-Rh2: 2 mg/kg; M-Rh2: 4 mg/kg; H-Rh2: 8 mg/kg), while the control group was only treated with thoracotomy and sodium chloride injection. Heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), and left ventricular end-diastolic pressure (LVEDP) were recorded by ultrasonic diagnosis. Rats were killed under anesthesia, and the morphological characteristics of ventricular tissue were observed by electron microscope. Additionally, cardiac blood and ventricular tissues were collected to quantify the contents of myocardial injury markers (creatine phosphate kinase (CK), creatine phosphokinase-MB isoenzyme (CK-MB), and lactate dehydrogenase (LDH) by ELISA, as well as the expression of pyroptosis-related genes cysteinyl aspartate specific proteinase 1 (Caspase-1), gasdermin D (GSDMD), interleukin (IL)-1β, and NOD-like receptor thermal protein domain associated protein 3 (NLRP3) by qRT-PCR and Western blot). Results. Ultrasonic examination identified lower HR, SBP, DBP, MAP, and LVSP in the three Rh2 injection groups compared with the control group (

P

 < 0.05); and in comparison with M- and H-Rh2 groups, HR, SBP, DBP, MAP, and LVSP were all lower in L-Rh2 group, while LVEDP was higher (

P

 < 0.05). Microscopically, CMs and organelles in the L-RH2, M-RH2, and H-RH2 groups were damaged to varying degrees compared with the control group, with those in the L-RH2 group being the most serious. CK, CK-MB, and LDH were also the highest in the L-Rh2 group and the lowest in the control group, while their levels were obviously reduced in M- and H-Rh2 groups (

P

 < 0.05). Finally, GSDMD, IL-1β, NLRP3, and Caspase-1 were found to be reduced in the control group, while pyroptosis-related gene expression in the M-Rh2 group was improved markedly (

P

 < 0.05). Conclusion. G-Rh2 can inhibit the pathological development of AMI by relieving the focal death of CM and inhibiting the release of proinflammatory factors in the body, and the effect is significantly related to the dosage, which is expected to become a new treatment option for AMI in the future.
Performance of Multilayered Nanocoated Cutting Tools in High-Speed Machining: A Review
Authors: Ganeshkumar S. , Venkatesh S. , Paranthaman P. , Arulmurugan R. , Arunprakash J. , Manickam M. , Venkatesh S. , Rajendiran G.
Published: October 2022

In machining processes, cutting tools play a dominant role in producing quality products. The quality of finished goods is directly related to the cutting tool condition. Several types of research have been carried out in cutting tool condition monitoring. On the other hand, the manufacturing industries should be aware of the cutting tool selection, operating conditions, and performance of cutting tools. This article emphasizes the performance of coated cutting tools and tool materials for various machining operations. Nowadays, the nanocoating of CNC tool inserts increases the wear resistance, vibration emissions, metal removal rate, etc. These coating techniques influence the manufacturing industry to increase the productivity and quality of the finished goods and reduce the machining cost. The performance of thin film multilayered coatings such as TiN, TiAlN, AlTiN, Ti, and TiCN on plain silicon carbide tool inserts is revealed by the researchers to guide the manufacturing industry for proper tool selection and standard machining inputs for metal removal operation. The influence of coating material such as TiBN, TiN, TiAlN, and CrAlSiN in cutting tools leads to increase the life time of the cutting tools, which decreases the material sticking and cutting forces. Titanium carbo nitride is wear-resistant and corrosion-resistant. Compared to TiCN, TiAlN is harder due to the higher hardness of 32 GPa. This article concludes the material selection based on the work piece material which yields good metal removal with less cutting forces. The article concludes the cutting material selection based on the work piece for machining operations.
Internet Plus Innovation and Entrepreneurship Education Model Based on Machine Learning Algorithms
Authors: Chen Xiaoxia
Published: October 2022

Innovation and entrepreneurship is a spiritual attitude. With the dissemination and wide application of the concept and technology of “Internet Plus,” Internet Plus innovative thinking has had a strong impact on innovation and entrepreneurship education, such as teaching concepts, changes in teaching methods, optimization of teaching staff and curriculum systems, and resource sharing. Therefore, according to the actual needs of innovation and entrepreneurship teaching in colleges and universities, this paper combines the concept of Internet and college education with the help of machine learning algorithms to build a new education system. First, the article designs the overall platform architecture and core business processes and develops and implements front-end and various platform functions. The system includes different modules such as preschool skill diagnosis, online learning, stage testing and evaluation, information and resource sharing, team building and project implementation management, and so on. Then, the experiment shows that the precision rate, recall rate, and F1 value of the system are all high, indicating that the system is stable and efficient. Finally, it briefly expounds the development strategies of the Internet Plus education model under the influence of relevant systems, which will help students and teachers develop innovation and entrepreneurship theories and improve students’ knowledge quality. The combination of Internet technology and innovation and entrepreneurship teaching can make innovation education more in line with the current learning needs and promote the improvement of entrepreneurship ability.
miR-124-3p Delivered Using Exosomes Attenuates the Keratinocyte Response to IL-17A Stimulation in Psoriasis
Authors: Liu Simin , Gong Jian
Published: October 2022

Background and Objective. To explore the effect of miR-124-3p delivered by exosomes on psoriatic keratinocytes stimulated by IL-17A. Methods. NHEKs, HaCaT cells, and HEK 293T cells were treated with IL-17A. CCK-8 assays were performed to detect cell activity, and immunofluorescence staining and Western blotting were performed to detect the protein expression of STAT3. After isolation of exosomes via ultracentrifugation, the contents of miR-124-3p and oxidative stress markers such as superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) in keratinocytes were measured. Subsequently, transcriptomic analysis was performed using RNA-seq. Data were analysed by using the “edgeR” package within R. After verifying the abnormally expressed genes stimulated by IL-17A, a dual luciferase reporter assay was used to determine the interaction between miR-124-3p and STAT3. Finally, BALB/c mice were used to establish a psoriasis model for analysis. The effect of elevated miR-124-3p on the psoriasis mouse model was determined by exosomal delivery of miR-124-3p. Results. IL-17 intervention enhanced the cell activity of keratinocytes (

P
<
0.05

). miR-124-3p was identified by RNA-seq as one of the differentially expressed miRNAs stimulated by IL-17A. miR-124-3p overexpression induced decreased STAT3 and MDA levels, increased SOD and GSH-Px levels in keratinocytes, and alleviated emergency responses of sclerosis damage (

P
<
0.05

). The dual luciferase reporter assay results confirmed that STAT3 was regulated by miR-124-3p in a targeted manner (

P
<
0.05

). Finally, miR-124-3p delivered by exosomes effectively alleviated the pathological manifestations and oxidative stress responses of psoriatic mice. Conclusions. miR-124-3p regulates keratinocyte activity via STAT3 in response to IL-17A stimulation. The ectopic expression of miR-124-3p in psoriatic skin reduces IL-17A-induced inflammation and inhibits the STAT3 pathway, thus alleviating the symptoms of psoriasis. The findings of this study suggest that exosomes can be used to therapeutically deliver miR-124-3p to keratinocytes and psoriatic lesions, which may provide novel insight for psoriasis treatment.
RNA Sequencing Reveals the Regulation Mechanism of Yunnan Baiyao in Treating Skin Infection Caused by Staphylococcus aureus
Authors: Zhang Jiachan , Wang Changtao , An Quan , Quan Qianghua , Wang Yiming , Huo Tong , Liu Jitao
Published: October 2022

Yunnan Baiyao is a well-known traditional Chinese medicine that can be formulated into a powder or capsule form. The mechanism by which it exerts its anti-inflammation effect, which is used in skin care products, needs to be further explored. In this study, we established the Staphylococcus aureus-induced mouse skin inflammatory model to investigate the effects of Yunnan Baiyao by the method of RNA-sequencing technology. The mice were randomly assigned to three groups, and those were control, model, and the Yunnan Baiyao-treated (YNtreated) group. Key genes and pathways were identified using bioinformatics analyses. In the study, we obtained 1,053 differentially expressed genes (DEGs) induced by Yunnan Baiyao. The 233 upregulated genes were enriched in 32 GO terms and 5 KEGG pathways, focused on the items, such as wound healing, cell metabolism, and proliferation, indicating the accelerating effects of Yunnan Baiyao on these aspects. The 820 downregulated genes were enriched mainly in the items, including the regulation of inflammation factor production, immune responses, and regulation of structure dermal components. Besides, Yunnan Baiyao reversed the expressions of 277 (201 decreased and 76 increased DEGs, respectively) induced by S. aureus. Ten key regulatory nodes (MMP2, PLK1, CCNB1, TLR4, CDK1, CCNA2, CDC25C, PDGFRA, MYOC, and KNG1) were identified by the construction of the protein interaction network, half of which were related to cell proliferation. VAV1 was another hub node that was affected by Yunnan Baiyao (Top 20). In the study, VAV1 and TLR4 can be considered key module genes in inflammation regulation. In conclusion, this study found that Yunnan Baiyao can significantly relieve inflammatory symptoms by regulating genes and pathways involved in the regulation of inflammation and immune response and also helped to deepen our understanding of the associated molecular mechanisms.
The Mediating Role of Alexithymia: Social Support and Depression among Colorectal Cancer Patients
Authors: Shi Hongyu , Wang Li , Zeng Ting , Li Yuewei , Xu Haiyan , Sui Xin , Gao Ruitong , Li Feng
Published: October 2022

Purpose. The purpose of this study was to investigate the relationship among depression, alexithymia, and social support and examine the main influence factors of depression in patients with colorectal cancer (CRC). In addition, the mediating role of alexithymia between social support and depression was analyzed. Methods. Patients with CRC were recruited in two tertiary hospitals in Changchun via a convenience sampling method. All survivors were asked to complete self-report questionnaires assessing medical and demographic variables, depression, alexithymia, and social support. Results. A total of 183 patients with CRC were included. The results showed that education level and family monthly income were the main factors affecting depression patients with in CRC. Alexithymia was positively correlated with depression (

r
=
0.389

,

P
<
0.01

). Social support was negatively correlated with alexithymia (

r
=
−
0.322

,

P
<
0.01

) and depression (

r
=
−
0.316

,

P
<
0.01

). Moreover, alexithmia partially mediated the relationship between social support and depression, and the ratio of mediating effect with total effect was 23.82%. Conclusions. To sum up, there is a correlation between depression, social support, and alexithymia in patients with CRC; alexithymia plays a partially intermediary role in the impact of social support on depression. Our findings remind clinicians to treat the depression of patients with CRC from multiple perspectives, such as by increasing social support and reducing alexithymia.
Integrin α6 Indicates a Poor Prognosis of Craniopharyngioma through Bioinformatic Analysis and Experimental Validation
Authors: Jia Yanfei , Wu Wentao , Xiao Youchao , Cai Kefan , Gui Songbai , Li Qiang , Li Tian
Published: October 2022

Background. Craniopharyngioma (CP) is a benign slow-growing tumor. It tends to affect children, and the number of patients is on rise. Considering the high morbidity and mortality of CP, it is urgent and pivotal to identify new biomarkers to uncover the etiology and pathogenesis of CP. Methods. The “limma” package was utilized to calculate the data from the Gene Expression Omnibus (GEO) database. Based on differentially expressed genes (DEGs), gene ontology and pathway analysis were deduced from the DAVID web tool. Further, we constructed a protein-protein interaction (PPI) network. Weighted correlation network analysis (WGCNA) was utilized to build a coexpression network. Finally, Western blotting and survival analysis were performed to examine the expression level of important metabolism-related genes. Results. Three hundred and eighty-four DEGs were identified between normal tissues and CPs from the GSE94349 and GSE26966 datasets. The Venn diagram for DEGs and hub genes in the ‘turquoise’ module revealed four key genes. Finally, the outcome of the survival analysis suggested that Integrin α6 (ITGA6) significantly affected the overall survival time of the patients with CP. Conclusion. IGTA6, as a metabolism-related molecule, was found to be substantially related to the overall survival of patients with CP.
Generation and Research of Online English Course Learning Evaluation Model Based on Genetic Algorithm Improved Neural Set Network
Authors: Song Qiuping
Published: October 2022

The rationality and timeliness of the comprehensive results of English course learning quality are increasingly important in the process of modern education. There are some problems in the scientific evaluation of English course learning quality and teachers’ own English course learning, such as the need for proper adjustment and improvement. Based on the improved network theory of genetic algorithm, this paper takes an online English course learning quality evaluation model and uses MATLAB 7.0 to write the graphical user interface of the neural set network English course learning quality prediction model. The model uses the genetic algorithm of adaptive mutation to optimize the initial weights and values of the neural set network and solves the problems of prediction accuracy and convergence speed of English course learning quality evaluation results. Simulation experiments show that the neural set network has a strong dependence on the initial weights and thresholds. Using the improved genetic algorithm to optimize the initial weights and thresholds of the neural set network reduced the time for the neural set network to find the weights and thresholds that meet the training termination conditions, the prediction accuracy was increased to 0.897, the prediction accuracy was 78.85%, and the level prediction accuracy was 84.62%, which effectively promoted the development of online English course learning in colleges and the continuous improvement of teachers’ English course learning level.