bluuebunny/crossref_metadata_2025 huggingface dataset:
Analytical Derivation and Analysis of Vertical and Lateral Installation Ratios for Swing Axle, McPherson and Double Wishbone Suspension Architectures
Authors: Bucchi Francesco , Lenzo Basilio
Published: August 2022

In the context of suspension design, the installation ratio (or motion ratio) is a parameter that relates wheel movement with spring deflection, quite an important kinematic property of a suspension. Yet, no study in the literature provides a clear relationship between the installation ratio and the geometrical features of a suspension. This paper employs rigid body kinematics and appropriate geometrical schematics to fill such a gap. Analytical expressions of the installation ratio are derived for three suspension layouts: swing axle, McPherson, double wishbone. Key concepts such as instant center, roll center and camber gain are harnessed to provide insightful analyses for relevant case studies of suspension passenger cars. Among the key results, the typical assumption of a McPherson installation ratio close to 1 is supported by a formal demonstration, and the new concept of “lateral” installation ratio is introduced which, alongside the classical “vertical” installation ratio, further characterizes suspension motion. Numerical results obtained through a multibody software support the findings of this paper. In conclusion, this study provides valuable insights for suspension design engineers.
Investigating the Relationship between Experience, Well-Being, and Loyalty: A Study of Wellness Tourists
Authors: Singh Ramjit , Manhas Parikshat Singh , Mir Mudasir Ahmad , Quintela Joana A.
Published: August 2022

The present research aims to determine the relationship between the construct of autonomy, intrinsic motivation, and tourist experience, as well as loyalty in the context of wellness and spa tourism. The exploratory–descriptive design was adopted in the present research. A research instrument was developed based on previous literature and was tested for a pilot study to check validity and reliability. A purposive sampling technique was used to collect the data from tourists who have gained spa experience. A total of 264 usable responses were received after distributing 400 questionnaires. The data were screened, processed, and analyzed using the SPSS 22.0 and Smart-PLS 2.0. The investigation showed that intrinsic motivation, autonomy, and experience positively impact positive emotions and life satisfaction. Besides, it also supported that positive emotions, experience, and life Satisfaction lead to loyalty. Furthermore, the present study tested the mediating role of experience, positive emotions, and life satisfaction; it was also found that experience mediates the relationship between autonomy, positive emotions, and life satisfaction. Positive emotions and life satisfaction were mediators in the relationship between experience and loyalty. Finally, the findings showed that destination images moderated the relationship between experience, positive emotions, and life satisfaction. The results of this investigation can be helpful for both the research community and marketers interested in investigating the well-being of tourists and destination loyalty.
A Typology and Preferences for Pigeon Pea in Smallholder Mixed Farming Systems in Uganda
Authors: Namuyiga Dorothy Birungi , Stellmacher Till , Borgemeister Christian , Groot Jeroen C. J.
Published: August 2022

Pigeon pea (Cajanus cajan) remains an under-researched ‘orphan crop’ yet is important for food and nutrition security of smallholders. Furthermore, smallholders are heterogeneous, with varying perceptions and resource capacities. Against this backdrop, we clustered smallholders and assessed their preferences for pigeon pea. Data were gathered through a cross-sectional survey of 257 smallholders from northern Uganda, corroborated with key informant interviews. Using multivariate analysis, we generated six clusters that explained 63% of the total variance. Three farm types (LEX—low-resourced and experienced, LUN—low-resourced and inexperienced, and LED—low-resourced and educated) represented 15, 10, and 17% of the farms, respectively, and were resource-constrained with low farm size, low livestock units, low education level and low pigeon pea sales. Three other types (MEX—medium-resourced and experienced, HEX—high-resourced and experienced, HED—high-resourced and educated) represented 7, 6, and 7% of the samples and were highly-resourced given their above-average level of endowment. Pigeon pea was mainly produced for household consumption, especially by the low-resourced farm types. Across farm types, smallholders preferred pigeon pea because it fixed nitrogen (94%), is relatively easy to harvest (90%) and it provided more biomass (89%) compared to other crops. The developed typology allows for tailored pro-poor agricultural policies to address particular necessities of specific farm types. Understanding varied preferences can facilitate investments into improving pigeon pea traits, which are particularly desirable and appropriate for smallholders.
Assessment of Laser Scanner Use under Different Settings in Two Differently Managed Vineyards for Estimating Pruning Wood Parameters
Authors: Anastasiou Evangelos , Balafoutis Athanasios , Theocharis Serafeim , Theodorou Nikolaos , Koundouras Stefanos , Fountas Spyros
Published: August 2022

Precision viticulture employs various sensors for assessing nondestructively key parameters in vineyards. One of the most promising technologies for this purpose is the laser scanner sensor. Laser scanner uses the LiDAR (Light Detection And Ranging) method for the calculation of the distance from the sensor. However, the number of cultivation operations affects the credibility of sensors such as the laser scanner. The main aim of this study was to assess a laser scanner sensor at different measurement settings for estimating pruning wood parameters on two wine grape cultivars (Sauvignon Blanc and Syrah) that received different numbers of farming interventions. The experiment was conducted in the two vineyards situated in the same farm for two successive years (2014 and 2015). The results indicated that the use of a laser scanner in the Syrah vineyard presented more accurate results (r = 0.966 in 2014 and r = 0.806 in 2015) when compared to the Sauvignon Blanc one (r = 0.839 in 2014 and r = 0.607 in 2015) regarding pruning wood parameters estimation. Different measurement settings and weather conditions had different effects on the accuracy of the sensor. It can be concluded that the laser scanner is a very helpful sensor for estimating pruning wood parameters in vineyards.
New Flowering and Architecture Traits Mediated by Multiplex CRISPR-Cas9 Gene Editing in Hexaploid Camelina sativa
Authors: Bellec Yannick , Guyon-Debast Anouchka , François Tracy , Gissot Lionel , Biot Eric , Nogué Fabien , Faure Jean-Denis , Tepfer Mark
Published: August 2022

Adapting plants to sustainable cropping systems is a major challenge for facing climate change and promoting agroecological transition. Camelina sativa is an emerging oilseed crop species with climate-resilient properties that could be used in double-cropping systems, in particular as a summer catch crop. The availability of early-flowering camelina is essential in such cropping systems to allow full completion of the growth cycle during summer. Targeted induced gene variation (TIGV) was used in camelina on several flowering repressor genes identified in Arabidopsis to obtain early-flowering lines. Multiplex editing of 15 target genes representing FLOWERING LOCUS C, SHORT VEGETATIVE PHASE, LIKE HETEROCHROMATIN PROTEIN 1, TERMINAL FLOWER 1 and EARLY FLOWERING LOCUS 3 induced combinatorial mutations that were screened for early-flowering phenotypes. Certain mutants showing a stable early-flowering trait after five generations also presented additional phenotypes: determinate flowering, shorter stature and/or basal branching. Different combinations of mutations had a positive or negative impact on yield. This work demonstrates that efficient multiplex CRISPR is achievable in hexaploid plants like camelina, providing valuable genetic diversity for better selecting lines adapted to new cropping systems.
Short-Term Effect of Biochar on Soil Organic Carbon Improvement and Nitrous Oxide Emission Reduction According to Different Soil Characteristics in Agricultural Land: A Laboratory Experiment
Authors: Lee Jong-Mun , Park Do-Gyun , Kang Seong-Su , Choi Eun-Jung , Gwon Hyo-Suk , Lee Hyoung-Seok , Lee Sun-Il
Published: August 2022

Biochar application has been considered as a promising solution to address the effects of modern agriculture on climate change. However, there is a lack of research on the biochar application of greenhouse gas emissions based on poor soils in Korean agricultural land. Therefore, this study aimed to evaluate the effects of biochar application according to different soil characteristics on soil organic carbon (SOC) improvement and greenhouse gas reduction. The incubation experiments were conducted for 49 days and used different feedstock (barley straw and poultry manure) and biochar application rates (0, 5, 10, and 20-ton ha−1) in four soil characteristics (upland, U; greenhouse, G; converted land, C; reclaimed land, R). The results of this study showed that the SOC increased significantly in all soils after biochar application. The increasing SOC rate was the highest in poor soil. Biochar 20-ton ha−1 treatment significantly reduced N2O emissions by 33.2% compared with the control. Barley straw biochar significantly reduced N2O emissions from all soils. Barley straw biochar decreased approximately 74.5% of N2O emissions compared with poultry manure biochar. Poultry manure biochar improved SOC and reduced N2O emissions in poor soil. However, in poultry manure biochar treatment in U and G soil, N2O emissions increased. In conclusion, barley straw biochar application was found to suppress N2O emissions and improve the SOC in all soil characteristics of agricultural land. In addition, the soil carbon storage effect and N2O reduction effect of biochar were the highest in poor soil. Thus, the biochar application can be a potential agricultural practice for improving soil quality and decreasing N2O emissions in domestic agricultural soil.
Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens
Authors: Huang Xuan , Zhang Haiyang , Cao Haiyue , Zhou Wei , Xiang Xin , Yin Zhaozheng
Published: August 2022

Egg production is a pivotal indicator for evaluating the fertility of poultry, and the ovary is an essential organ for egg production and plays an indispensable role in poultry production and reproduction. In order to investigate different aspects of egg production mechanisms in different poultry, in this study we performed a metabolomic analysis of the transcriptomic combination of the ovaries of two chicken breeds, the high-production Ninghai indigenous chickens and the low-production Wuliangshan black-boned chickens, to analyze the biosynthesis and potential key genes and metabolic pathways in the ovaries during egg production. We predicted four genes in the transcriptomic that are associated with egg production, namely P2RX1, INHBB, VIPR2, and FABP3, and identified three important pathways during egg production, “Calcium signaling pathway”, “Neuroactive ligand–receptor interaction” and “Cytokine–cytokine receptor interaction”, respectively. In the metabolomic 149 significantly differential metabolites were identified, 99 in the negative model and 50 in the positive model, of which 17α-hydroxyprogesterone, iloprost, spermidine, and adenosine are important metabolites involved in reproduction. By integrating transcriptomics and metabolomics, the correlation between specific differential genes and differential metabolites identified important gene-metabolite pairs “VIPR2-Spermidine” and “P2RX1-Spermidine” in egg production. In conclusion, these data provide a better understanding of the molecular differences between the ovaries of low- and high-production hens and provide a theoretical basis for further studies on the mechanics of poultry egg production.
Cloning and Expression Analysis of HAT1 and HDAC1 in the Testes of Mature Yaks and Their Sterile Hybrids
Authors: Sun Shijie , Shen Zhenhua , Jin Suyu , Huang Lin , Zheng Yucai
Published: August 2022

The objective of this study was to explore the molecular mechanism of male sterility in yak hybrids based on HAT1 and HDAC1. Total RNA was extracted from the testes of adult yaks (n = 11) and sterile cattle-yaks (n = 11) followed by reverse transcription. The coding sequence (CDS) of yak HAT1 and HDAC1 were obtained by conventional polymerase chain reaction (PCR) and gene cloning. The testicular mRNA and protein levels of HAT1 and HDAC1 in yaks and cattle-yaks were detected by quantitative PCR (qPCR) and Western blotting, respectively, and the histone H3 lysine 9 (H3K9) histone acetylation level in the testes of yaks and cattle-yaks was assayed using enzyme linked immunosorbent assay (ELISA). The results showed that the CDS of HAT1 and HDAC1 were 1242 bp and 1449 bp in length, encoding 413 and 482 amino acids, respectively; yaks had a similar mRNA sequence as cattle in both genes. The testicular mRNA and protein levels of HAT1 of cattle-yaks were significantly lower than those of yaks, and the protein level of HDAC1 was significantly higher than that of yaks. ELISA showed that the acetylation level of testicular H3K9 was significantly lower in yak hybrids than that of yaks. The present results suggest that the decreased level of HAT1 and increased level of HDAC1 may result in the decreased H3K9 acetylation in cattle-yaks and might be associated with their sterility.
The Effect of Phytonutrients in Terminalia chebula Retz. on Rumen Fermentation Efficiency, Nitrogen Utilization, and Protozoal Population in Goats
Authors: Gunun Pongsatorn , Cherdthong Anusorn , Khejornsart Pichad , Wanapat Metha , Polyorach Sineenart , Kang Sungchhang , Kaewwongsa Walailuck , Gunun Nirawan
Published: August 2022

The aim of this study was to investigate the effect of Terminalia chebula meal (TCM) supplementation on digestibility, rumen fermentation, nitrogen utilization, and protozoal population in goats. Eight goats with an initial body weight (BW) of 13 ± 3.0 kg were randomly assigned according to a double 4 × 4 Latin square design to receive different levels of TCM supplementation at 0, 8, 16, and 24 g/kg of total dry matter (DM) intake, respectively. The goats were fed with concentrate diets at 13 g/kg BW, while rice straw was used as a roughage source, fed ad libitum. The results revealed that the feed intake and the apparent digestibility of DM, organic matter (OM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) were similar among the treatments (p > 0.05). However, crude protein (CP) digestibility decreased significantly (p < 0.05) when supplemented with TCM at 24 g/kg of total DM intake (p < 0.05). The addition of TCM did not change the ruminal pH and blood urea nitrogen concentrations (p > 0.05), whereas the concentration of NH3-N at 4 h post feeding was reduced with the inclusion of TCM at 16 and 24 g/kg of total DM intake. The total numbers of bacteria were enhanced by the addition of TCM, while the protozoal population, in both entodiniomorph and holotrich, was reduced (p < 0.05). The supplementation of TCM did not change the concentration of total volatile fatty acids (TVFA), acetic acid, or butyric acid, while the propionic acid concentration at 4-h post feeding increased significantly, especially when supplemented at 16 g/kg of total DM intake (p < 0.05. In addition, urinary nitrogen (N) excretion decreased, while fecal N excretion, N absorption, N retention, and the proportion of N retention to N intake increased with the inclusion of TCM at all levels. In summary, the inclusion of TCM could improve rumen fermentation efficiency and N balance without having an adverse effect on feed intake, nutrient digestibility, and rumen ecology; however, the protozoal population decreased. Therefore, this study suggests that TCM (16 g/kg of total DM intake) could be used as a plant source for rumen enhancement in goats fed a diet based on rice straw without having an adverse effect on feed intake or nutrient digestion. However, further studies on the production of types of meat and milk that have a long-term feeding trial should be carried out.
Estimation of Genetic Parameters for Heifer and Cow Fertility Traits Derived from On-Farm AI Service Records of South African Holstein Cattle
Authors: Kgari Ramadimetje Delight , Muller Carel , Dzama Kennedy , Makgahlela Mahlako Linah
Published: August 2022

This study aimed to derive additional fertility traits from service data and estimate their genetic parameters for their possible inclusion in the South African Holstein cattle breeding programs. Service records (n = 64,464) were collected from 18 South African Holstein herds using on-farm automated milk recording systems. Using a multivariate model, the data were used to estimate heritabilities and correlations among several fertility traits. The pedigree data consisted of information on 18,592 animals born between 1981 and 2013. Heritability estimates observed were low to moderate (0.02 ± 0.00 to 0.24 ± 0.00), indicating that there is some genetic basis for the explored fertility traits to warrant selection. The genetic correlations observed between fertility traits were generally favorable, with some high correlations between age at first service (AFS) and services per conception for heifers (SPCh) (0.73 ± 0.00) and between days from calving to first service (CFS) and services per conception for cows (SPC) (0.90 ± 0.01). Positive genetic correlations indicate that improvement in one trait is coupled with a correlated genetic increase in another trait. The studied fertility traits could be used in addition to AFC and CI to serve as a basis for the selection of reproduction in dairy cattle to minimize selection bias.
Is Dairy Effluent an Alternative for Maize Crop Fertigation in Semiarid Regions? An Approach to Agronomic and Environmental Effects
Authors: Lombardi Banira , Orden Luciano , Varela Patricio , Garay Maximiliano , Iocoli Gastón Alejandro , Montenegro Agustín , Sáez-Tovar José , Bustamante María Ángeles , Juliarena María Paula , Moral Raul
Published: August 2022

The reuse of effluents from intensive dairy farms combined with localized irrigation techniques (fertigation) has become a promising alternative to increase crop productivity while reducing the environmental impact of waste accumulation and industrial fertilizers production. Currently, the reuse of dairy effluents through fertigation by subsurface drip irrigation (SDI) systems is of vital importance for arid regions but it has been poorly studied. The present study aimed to assess the greenhouse gas (GHG) emissions, soil properties, and crop yield of a maize crop fertigated with either treated dairy effluent or dissolved granulated urea applied through an SDI system at a normalized N application rate of 200 kg N ha−1. Fertilizer application was divided into six fertigation events. GHG fluxes were measured during fertigation (62-day) using static chambers. Soil properties were measured previous to fertilizer applications and at the harvest coinciding with crop yield estimation. A slight increase in soil organic matter was observed in both treatments for the 20–60 cm soil depth. Both treatments also showed similar maize yields, but the dairy effluent increased net GHG emissions more than urea during the fertigation period. Nevertheless, the net GHG emissions from the dairy effluent were lower than the theoretical CO2eq emission that would have been emitted during urea manufacturing or the longer storage of the effluent if it had not been used, showing the need for life-cycle assessments. Local-specific emission factors for N2O were determined (0.07%), which were substantially lower than the default value (0.5%) of IPCC 2019. Thus, the subsurface drip irrigation systems can lead to low GHG emissions, although further studies are needed.
Effects of Heat Stress in Dairy Cows Offered Diets Containing Either Wheat or Corn Grain during Late Lactation
Authors: Garner Josie B. , Williams S. Richard O. , Moate Peter J. , Jacobs Joe L. , Hannah Murray C. , Morris Greg L. , Wales William J. , Marett Leah C.
Published: August 2022

Cereal grains that differ in the rate and extent of ruminal fermentation differ in heat increment and may be used to improve thermoregulation during heat stress. This experiment investigated the responses of dairy cows in late lactation to a heat challenge when offered wheat-grain or corn-grain. Eighteen lactating cows, 220 ± 94 (mean ± standard deviation) days in milk, 3.7 ± 0.17 years of age and 558 ± 37 kg bodyweight, were allocated treatments containing 6 kg dry matter (DM)/day of wheat grain or 6 kg DM/day corn grain (9 per treatment) plus 14 kg DM/day of alfalfa hay. Measurements were made during a 7-day pre-challenge period at ambient conditions in individual stalls, during a 4-day heat challenge (temperature humidity index of 74 to 84) in individual controlled-climate chambers, then during a 7-day recovery period at ambient conditions in individual stalls. During the heat challenge, cows offered corn had lower respiration rates (p = 0.017) and greater feed intake (p = 0.021) but energy-corrected milk (p = 0.097) was not different to that of cows offered wheat. Feeding corn grain to dairy cows during a heat challenge reduced some of the negative impacts of heat stress, enabling the cows to consume more forage compared with supplementing with wheat grain.
Schizothorax prenanti Heat Shock Protein 27 Gene: Cloning, Expression, and Comparison with Other Heat Shock Protein Genes after Poly (I:C) Induction
Authors: Zhang Jianlu , Zhang Kunyang , Huang Jiqin , Jiang Wei , Ma Hongying , Deng Jie , Zhang Hongxing , Li Wanchun , Wang Qijun
Published: August 2022

We identified and cloned cDNA encoding the heat shock protein (Hsp) 27 gene from Schizothorax prenanti (SpHsp27), and compared its expression with that of SpHsp60, SpHsp70, and SpHsp90 in the liver, head kidney, hindgut, and spleen of S. prenanti that were injected with polyinosinic-polycytidylic acid [Poly (I:C)]. The SpHsp27 partial cDNA (sequence length, 653 bp; estimated molecular mass, 5.31 kDa; theoretical isoelectric point, 5.09) contained an open reading frame of 636 bp and a gene encoding 211 amino acids. The SpHsp27 amino acid sequence shared 61.0–92.89% identity with Hsp27 sequences from other vertebrates and SpHsp27 was expressed in seven S. prenanti tissues. Poly (I:C) significantly upregulated most SpHsps genes in the tissues at 12 or 24 h (p < 0.05) compared with control fish that were injected with phosphate-buffered saline. However, the intensity of responses of the four SpHsps was organ-specifically increased. The expression of SpHsp27 was increased 163-fold in the head kidney and 26.6-fold SpHsp27 in the liver at 24 h after Poly (I:C) injection. In contrast, SpHsp60 was increased 0.97–1.46-fold in four tissues and SpHsp90 was increased 1.21- and 1.16-fold in the liver and spleen at 12 h after Poly (I:C) injection. Our findings indicated that Poly (I:C) induced SpHsp27, SpHsp60, SpHsp70, and SpHsp90 expression and these organ-specific SpHsps are potentially involved in S. prenanti antiviral immunity or mediate pathological process.
Antibiotic Resistance in Bacteria—A Review
Authors: Urban-Chmiel Renata , Marek Agnieszka , Stępień-Pyśniak Dagmara , Wieczorek Kinga , Dec Marta , Nowaczek Anna , Osek Jacek
Published: August 2022

Background: A global problem of multi-drug resistance (MDR) among bacteria is the cause of hundreds of thousands of deaths every year. In response to the significant increase of MDR bacteria, legislative measures have widely been taken to limit or eliminate the use of antibiotics, including in the form of feed additives for livestock, but also in metaphylaxis and its treatment, which was the subject of EU Regulation in 2019/6. Numerous studies have documented that bacteria use both phenotypis and gentic strategies enabling a natural defence against antibiotics and the induction of mechanisms in increasing resistance to the used antibacterial chemicals. The mechanisms presented in this review developed by the bacteria have a significant impact on reducing the ability to combat bacterial infections in humans and animals. Moreover, the high prevalence of multi-resistant strains in the environment and the ease of transmission of drug-resistance genes between the different bacterial species including commensal flora and pathogenic like foodborne pathogens (E. coli, Campylobacter spp., Enterococcus spp., Salmonella spp., Listeria spp., Staphylococcus spp.) favor the rapid spread of multi-resistance among bacteria in humans and animals. Given the global threat posed by the widespread phenomenon of multi-drug resistance among bacteria which are dangerous for humans and animals, the subject of this study is the presentation of the mechanisms of resistance in most frequent bacteria called as “foodborne pathoges” isolated from human and animals. In order to present the significance of the global problem related to multi-drug resistance among selected pathogens, especially those danger to humans, the publication also presents statistical data on the percentage range of occurrence of drug resistance among selected bacteria in various regions of the world. In addition to the phenotypic characteristics of pathogen resistance, this review also presents detailed information on the detection of drug resistance genes for specific groups of antibiotics. It should be emphasized that the manuscript also presents the results of own research i.e., Campylobacter spp., E. coli or Enetrococcus spp. This subject and the presentation of data on the risks of drug resistance among bacteria will contribute to initiating research in implementing the prevention of drug resistance and the development of alternatives for antimicrobials methods of controlling bacteria.
C-Locked Analogs of the Antimicrobial Peptide BP214
Authors: Andersen Ida Kristine Lysgaard , Thomsen Thomas T. , Rashid Jasmina , Bobak Thomas Rønnemoes , Oddo Alberto , Franzyk Henrik , Løbner-Olesen Anders , Hansen Paul R.
Published: August 2022

BP214 is an all-D antimicrobial peptide amide, kklfkkilryl, which shows an excellent activity against colistin-resistant Acinetobacter baumannii and a low hemolytic activity. The aim of the present work was to investigate how C-terminus-to-side chain macrocyclization and fatty acid modification affect the antimicrobial and hemolytic activity of this peptide. In total, 18 analogs of BP214 were synthesized using a combination of Fmoc-based solid-phase peptide synthesis and the submonomer approach. Cyclization was achieved by reacting the ε-amino group of a C-terminal lysine residue with a bromoacetylgroup attached to the Nα amino group of the N-terminal amino acid, generating a secondary amine at which the exocyclic lipopeptide tail was assembled. Three different ring sizes (i.e., 3–5 amino acid residues) of C-locked analogs combined with fatty acids of different lengths (i.e., C10–C14) were investigated. The antimicrobial activity of the analogs was tested against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa. The most promising compound was analog 13 (MIC = 4 µg/mL (2.4 µM) against E. coli and 36% hemolysis of red blood cells at 150 µM). In a time-kill assay, this peptide showed a significant, concentration-dependent reduction in viable E. coli cells comparable to that seen for colistin.
Design and Evaluation of Short Bovine Lactoferrin-Derived Antimicrobial Peptides against Multidrug-Resistant Enterococcus faecium
Authors: Mishra Biswajit , Felix LewisOscar , Basu Anindya , Kollala Sai Sundeep , Chhonker Yashpal Singh , Ganesan Narchonai , Murry Daryl J. , Mylonakis Eleftherios
Published: August 2022

Enterococcus faecium has become an important drug-resistant nosocomial pathogen because of widespread antibiotic abuse. We developed short and chemically simple antimicrobial peptides (AMPs) with a selective amino acid composition, fixed charge, and hydrophobicity ratio based on the core antimicrobial motif of bovine lactoferrin (LfcinB6). Among these peptides, 5L and 6L (both 12 residues long) demonstrated a narrow spectrum and high antibacterial activity against drug-resistant E. faecium isolates with a minimal inhibitory concentration (MIC) that ranged from 4–16 µg/mL. At 32 µg/mL, peptides 5L and 6L inhibited E. faecium strain C68 biofilm formation by 90% and disrupted established biofilms by 75%. At 40 µg/mL, 5L reduced 1 × 107E. faecium persister cells by 3 logs within 120 min of exposure, whereas 6L eliminated all persister cells within 60 min. At 0.5× MIC, 5L and 6L significantly downregulated the expression of a crucial biofilm gene ace by 8 folds (p = 0.02) and 4 folds (p = 0.01), respectively. At 32 µg/mL, peptides 5L and 6L both depolarized the E. faecium membrane, increased fluidity, and eventually ruptured the membrane. Physiologically, 5L (at 8 µg/mL) altered the tricarboxylic acid cycle, glutathione, and purine metabolism. Interestingly, in an ex vivo model of porcine skin infection, compared to no treatment, 5L (at 10× MIC) effectively eliminated all 1 × 106 exponential (p = 0.0045) and persister E. faecium cells (p = 0.0002). In conclusion, the study outlines a roadmap for developing narrow-spectrum selective AMPs and presents peptide 5L as a potential therapeutic candidate to be explored against E. faecium.
Antioxidant Capacities and Enzymatic Inhibitory Effects of Different Solvent Fractions and Major Flavones from Celery Seeds Produced in Different Geographic Areas in China
Authors: Zhang Chao , Yu Jing , Tu Qiang , Yan Fu , Hu Zhao , Zhang Youming , Song Chun
Published: August 2022

To extend the application of celery (Apium graveolens L.) seeds, the antioxidant and enzymatic inhibitory activities of different fractions and their main flavones were investigated. The n-butanol fractions possessed the highest total phenolic content (TPC) and total flavonoid content (TFC) values. The n-butanol fractions from Northeast China samples exhibited the strongest free radical scavenging (DPPH IC50 = 20.27 μg/mL, ABTS IC50 = 15.11 μg/mL) and ferric reducing antioxidant power (FRAP 547.93 mg trolox (TE)/g) capacity, while those collected from Hubei China showed the optimal cupric reducing antioxidant capacity (CUPRAC) values (465.78 mg TE/g). In addition, the dichloromethane fractions from Jiangsu samples displayed a maximum Fe2+ chelating capacity (20.81 mg ethylene diamine tetraacetic acid (EDTA)/g). Enzyme level experiments indicated polyphenolic compounds might be the main hypoglycemic active components. Subsequently, the enzyme inhibitory activity of nine main flavones was evaluated. Chrysoeriol-7-O-glucoside showed better α-glucosidase inhibitory activity than others. However, apigenin showed the best inhibitory effect on α-amylases, while the presence of glycosides would reduce its inhibitory effect. This study is the first scientific report on the enzymatic inhibitory activity, molecular docking, and antioxidant capacity of celery seed constituents, providing a basis for treating or preventing oxidative stress-related diseases and hyperglycemia.
The Phytochemical and Nutritional Composition of Shallot Species (Allium × cornutum, Allium × proliferum and A. cepa Aggregatum) Is Genetically and Environmentally Dependent
Authors: Major Nikola , Perković Josipa , Palčić Igor , Bažon Iva , Horvat Ivana , Ban Dean , Goreta Ban Smiljana
Published: August 2022

Shallots are a perennial plant from the Alliaceae family, classified with the common onion under the name of the Allium cepa Aggregatum group. The term shallot is also used for diploid and triploid viviparous onions, known as Allium × proliferum (Moench) Schrad and Allium × cornutum Clementi ex Vis., respectively. In this study, we compared the dry matter, pyruvic acid content, sugar content, flavonoid content, antioxidant capacity and mineral composition of 34 shallot accessions falling into three shallot species (Allium × cornutum, Allium × proliferum and A. cepa Aggregatum). Shallot accessions belonging to the A.× cornutum and A. × proliferum groups are characterized by high dry matter content (around 25%), of which a little less than 50% is formed of inulin-type sugars, polysaccharides, considered an excellent prebiotic with beneficial effects on human health. On the other hand, accessions belonging to the A. cepa Aggregatum group have lower dry matter content and, as a result, lower pungency (measured as pyruvic acid content), making them more suitable for fresh consumption by a broader range of consumers, but, at the same time, abundant in phenolic compounds, especially quercetin and isorhamnetin glycosides. We also observed a greater biodiversity among accessions within the A. cepa Aggregatum group in all the analyzed physico-chemical parameters compared to the other shallot groups. The investigated shallot accessions have an excellent in vitro antioxidant capacity, as well as excellent nutritional properties.
Cytoglobin Silencing Promotes Melanoma Malignancy but Sensitizes for Ferroptosis and Pyroptosis Therapy Response
Authors: De Backer Joey , Maric Darko , Zuhra Karim , Bogaerts Annemie , Szabo Csaba , Vanden Berghe Wim , Hoogewijs David
Published: August 2022

Despite recent advances in melanoma treatment, there are still patients that either do not respond or develop resistance. This unresponsiveness and/or acquired resistance to therapy could be explained by the fact that some melanoma cells reside in a dedifferentiated state. Interestingly, this dedifferentiated state is associated with greater sensitivity to ferroptosis, a lipid peroxidation-reliant, iron-dependent form of cell death. Cytoglobin (CYGB) is an iron hexacoordinated globin that is highly enriched in melanocytes and frequently downregulated during melanomagenesis. In this study, we investigated the potential effect of CYGB on the cellular sensitivity towards (1S, 3R)-RAS-selective lethal small molecule (RSL3)-mediated ferroptosis in the G361 melanoma cells with abundant endogenous expression. Our findings show that an increased basal ROS level and higher degree of lipid peroxidation upon RSL3 treatment contribute to the increased sensitivity of CYGB knockdown G361 cells to ferroptosis. Furthermore, transcriptome analysis demonstrates the enrichment of multiple cancer malignancy pathways upon CYGB knockdown, supporting a tumor-suppressive role for CYGB. Remarkably, CYGB knockdown also triggers activation of the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome and subsequent induction of pyroptosis target genes. Altogether, we show that silencing of CYGB expression modulates cancer therapy sensitivity via regulation of ferroptosis and pyroptosis cell death signaling pathways.
Enhanced Electrokinetic Remediation of Copper-Contaminated Soil by Combining Steel Slag and a Permeable Reactive Barrier
Authors: Chen Rong , Zhou Lu , Wang Weizhuo , Cui Denghui , Hao Dongxue , Guo Jingbo
Published: August 2022

Electrokinetic (EK) remediation methods can remove heavy metals from the soil, but the removal efficiency is generally low. In this paper, indoor remediation experiments of simulated copper-contaminated clay under four different types of electrolyte conditions (KCl, HAc, AC, and PASP (polyaspartic acid)) are carried out to validate the theory of an electrodynamically coupled steel slag permeability reactive wall (PRB). By comparison with EK remediation, it has been shown that the EK-PRB coupled remediation method can promote the removal of heavy metal copper in the soil, especially in the removal of reducible copper and exchangeable copper. The method can effectively avoid the increase in soil pH value and reduce the accumulation range of heavy metals while reducing the accumulation amount of heavy metals. This method has better energy utilization efficiency, and the unit energy consumption is smaller than the single electric remediation test.
Preparation of Al2O3 Multichannel Cylindrical-Tube-Type Microfiltration Membrane with Surface Modification
Authors: Naseer Danyal , Ha Jang-Hoon , Lee Jongman , Song In-Hyuck
Published: August 2022

Membrane technology has become a highly efficient separation technology for industrial applications over the past few decades. The key requirements for porous ceramic membranes are mechanical stability, controlled pore morphology, and high permeability. However, only a few studies have been conducted to optimise commercial membrane filters. In this study, a multichannel cylindrical-tube-type support with a microfiltration (MF) coating was prepared. To design a reliable porous structure and avoid shrinkage defects, the support layer was extruded using a combination of different-sized Al2O3 particles. The alumina microfiltration coating was developed using multiple dip-coatings to control the membrane thickness. An inorganic surface modification was conducted for the first time on an extruded membrane using a SiO2 sol-gel technique to enhance the antifouling properties. Furthermore, the membrane properties were investigated with scanning electron microscopy, mercury porosimetry, and a dead-end microfiltration system.
Water-Insoluble Black Pigment Released from the Octocoral Sinularia flexibilis
Authors: Kuo Fu-Wen , Chang Yu-Chia , Li Hsing-Hui
Published: August 2022

Coral reefs are the most diverse and productive marine ecosystems on earth. The National Museum of Marine Biology and Aquarium has cultured more than 35 species of corals for research. When we conducted the asexual propagation of corals, the octocoral Sinularia flexibilis released a black pigment that stained the operator’s hands black. This is the first reported case of the skin being dyed black while propagating corals. We quantified the blackness of the stain by using the RGB value of the color. The longer the coral contacted the skin, the darker the skin became. Incubating the tentacles of S. flexibilis in high-salinity filtered seawater increased the amount of the black pigment released. However, collecting 100% of the black pigment was exceedingly challenging because it was very sticky and was constantly entangled with Symbiodiniaceae. Furthermore, we were unable to identify any solvents that could dissolve the pigment. The structure and function of the black pigment merit further study as it has the potential to become a new black dye for human industries.
Combining Keyframes and Image Classification for Violent Behavior Recognition
Authors: Bi Yanqing , Li Dong , Luo Yu
Published: August 2022

Surveillance cameras are increasingly prevalent in public places, and security services urgently need to monitor violence in real time. However, the current violent-behavior-recognition models focus on spatiotemporal feature extraction, which has high hardware resource requirements and can be affected by numerous interference factors, such as background information and camera movement. Our experiments have found that violent and non-violent video frames can be classified by deep-learning models. Therefore, this paper proposes a keyframe-based violent-behavior-recognition scheme. Our scheme considers video frames as independent events and judges violent events based on whether the number of keyframes exceeds a given threshold, which reduces hardware requirements. Moreover, to overcome interference factors, we propose a new training method in which the background-removed and original image pair facilitates feature extraction of deep-learning models and does not add any complexity to the networks. Comprehensive experiments demonstrate that our scheme achieves state-of-the-art performance for the RLVS, Violent Flow, and Hockey Fights datasets, outperforming existing methods.
Diagnostic Accuracy of Multiple D-Dimer Cutoff Thresholds and Other Clinically Applicable Biomarkers for the Detection and Radiographic Evaluation of Pulmonary Embolism
Authors: Chrysikos Serafeim , Papaioannou Ourania , Karampitsakos Theodoros , Tavernaraki Kyriaki , Thanou Ioanna , Filippousis Petros , Anyfanti Maria , Hillas Georgios , Tzouvelekis Argyrios , Thanos Loukas , Dimakou Katerina
Published: August 2022

Background: Diagnostic work-up of pulmonary embolism (PE) remains a challenge. Methods: We retrospectively studied all patients referred for computed tomography pulmonary angiography (CTPA) with suspicion of PE during a 12-month period (2018). The diagnostic accuracy of different D-dimer (Dd) cutoff thresholds for ruling out PE was evaluated. Furthermore, the association of Dd and red cell distribution width (RDW) with embolus location, CTPA findings, and patient outcome was recorded. Results: One thousand seventeen (n = 1017) patients were finally analyzed (mean age: 64.6 years (SD = 11.8), males: 549 (54%)). PE incidence was 18.7%. Central and bilateral embolism was present in 44.7% and 59.5%, respectively. Sensitivity and specificity for conventional and age-adjusted Dd cutoff was 98.2%, 7.9%, and 98.2%, 13.1%, respectively. A cutoff threshold (2.1 mg/L) with the best (64.4%) specificity was identified based on Receiver Operating Characteristics analysis. Moreover, a novel proposed Dd cutoff (0.74 mg/L) emerged with increased specificity (20.5%) and equal sensitivity (97%) compared to 0.5 mg/L, characterized by concurrent reduction (17.2%) in the number of performed CTPAs. Consolidation/atelectasis and unilateral pleural effusion were significantly associated with PE (p < 0.05, respectively). Patients with consolidation/atelectasis or intrapulmonary nodule(s)/mass on CTPA exhibited significantly greater median Dd values compared to patients without the aforementioned findings (2.34, (IQR 1.29–4.22) vs. 1.59, (IQR 0.81–2.96), and 2.39, (IQR 1.45–4.45) vs. 1.66, (IQR 0.84–3.12), p < 0.001, respectively). RDW was significantly greater in patients who died during hospitalization (p = 0.012). Conclusions: Age-adjusted Dd increased diagnostic accuracy of Dd testing without significantly decreasing the need for imaging. The proposed Dd value (0.74 mg/L) showed promise towards reducing considerably the need of CTPA. Multiple radiographic findings have been associated with increased Dd values in our study.
Cauchy Integral and Boundary Value for Vector-Valued Tempered Distributions
Authors: Carmichael Richard D.
Published: August 2022

Using the historically general growth condition on scalar-valued analytic functions, which have tempered distributions as boundary values, we show that vector-valued analytic functions in tubes TC=Rn+iC obtain vector-valued tempered distributions as boundary values. In a certain vector-valued case, we study the structure of this boundary value, which is shown to be the Fourier transform of the distributional derivative of a vector-valued continuous function of polynomial growth. A set of vector-valued functions used to show the structure of the boundary value is shown to have a one–one and onto relationship with a set of vector-valued distributions, which generalize the Schwartz space DL2′(Rn); the tempered distribution Fourier transform defines the relationship between these two sets. By combining the previously stated results, we obtain a Cauchy integral representation of the vector-valued analytic functions in terms of the boundary value.
NRSF/REST-Mediated Epigenomic Regulation in the Heart: Transcriptional Control of Natriuretic Peptides and Beyond
Authors: Inazumi Hideaki , Kuwahara Koichiro
Published: August 2022

Reactivation of fetal cardiac genes, including those encoding atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), is a key feature of pathological cardiac remodeling and heart failure. Intensive studies on the regulation of ANP and BNP have revealed the involvement of numerous transcriptional factors in the regulation of the fetal cardiac gene program. Among these, we identified that a transcriptional repressor, neuron-restrictive silencer factor (NRSF), also named repressor element-1-silencing transcription factor (REST), which was initially detected as a transcriptional repressor of neuron-specific genes in non-neuronal cells, plays a pivotal role in the transcriptional regulation of ANP, BNP and other fetal cardiac genes. Here we review the transcriptional regulation of ANP and BNP gene expression and the role of the NRSF repressor complex in the regulation of cardiac gene expression and the maintenance of cardiac homeostasis.
Dynamics of Circulating CD14/CD16 Monocyte Subsets in Obstructive Sleep Apnea Syndrome Patients upon Hypoglossal Nerve Stimulation
Authors: Pries Ralph , Lange Christian , Behn Nicole , Bruchhage Karl-Ludwig , Steffen Armin
Published: August 2022

Background: Obstructive sleep apnea syndrome (OSAS) is a widespread respiratory disease that is associated with recurrent breathing intermissions at night. The corresponding oxidative stress triggers a low-grade systemic inflammation which leads to alterations of different immune cells in the peripheral blood. The current standard treatment for OSAS is continuous positive airway pressure (CPAP), whereas hypoglossal nerve stimulation (HNS) has been established as a second-line treatment option for CPAP failure. The aim of the study was to investigate the influence of HNS for OSAS patients on the distribution and differentiation of circulating monocyte subsets in connection with the clinical parameters. Materials and Methods: Therefore, a detailed analysis of the distribution of CD14/CD16 characterized monocyte subsets in the peripheral blood of OSAS patients before and after HNS therapy was performed by flow cytometry. Furthermore, values of BMI (body mass index), ODI (oxygen desaturation index), and ESS (Epworth Sleepiness Scale) were measured. Results: These OSAS patients significantly improved AHI and ESS scores under HNS. In addition, HNS revealed the potential to ensure normal distributions of blood monocyte subsets and even improved the monocyte dynamics in selected OSAS patients, but there were no significant correlations with AHI, ODI, HNS usage, and daytime sleepiness. Conclusions: We conclude that HNS-related positive effects on the oxygenation of the peripheral blood as well as affect the distribution of circulating monocyte subsets, but clinical OSAS correlations are missing. Far more individual clinical, cellular and molecular factors are involved in this sensitive and complex regulatory network and have to be elucidated in further studies.