bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of silver nanoparticles inside leaf of European beech (Fagus sylvatica L.)
Authors: Ballikaya Paula , Mateos José María , Brunner Ivano , Kaech Andres , Cherubini Paolo
Published: January 2023

In a greenhouse experiment, silver nanoparticles (Ag-NPs) were applied on European beech (Fagus sylvatica L.) leaves using the droplet application method. Scanning electron microscopy (SEM) analyses showed that after 24 h silver nanoparticles were mostly present in aggregates or as single particles on the surface of the leaf, surrounding or covering the stomata. Analyses of cross sections of the leaf revealed that some silver nanoparticles were adhering to the cell walls of the mesophyll and palisade cells, most likely after penetration into the leaf through the stomata as particles and not as Ag ions. Our preliminary results showed evidence of foliar uptake of silver nanoparticles in European beech. This opens new insights on the ability of trees to take up solid nanosized particles, eventually contained in raindrops, through their leaves, and potentially transport them to other parts of the tree. This study would be helpful for investigating the role of trees in atmospheric ultrafine particle mitigation.
Assessment of topsoil removal as an effective method for vegetation restoration in farmed peatlands
Authors: Wang Yimeng , Wang Yixiao , Wang Shengzhong , Wang Ming , Chai Wenjie
Published: January 2023

Peatland areas have dramatically declined in the past century because of the demand for agriculture. Therefore, it is necessary to develop suitable techniques to preserve these unique ecosystems. We studied the effects of topsoil removal on vegetation restoration in silt- and sand-amended peatlands in Changbai Mountain, China. We observed that topsoil removal effectively improved soil nutrient levels and water holding capacity in the silt-amended peatland but exhibited no significant effect on the sand-amended peatland. Topsoil removal decreased the species richness in both silt- and sand-amended peatlands but did not have any effect on the plant cover and biomass in the sand-amended peatland. The coverage, density, and aboveground biomass of dominant species, namely, Carex schmidtii, significantly increased after topsoil removal in the silt-amended peatland. The target Carex species was absent from the sand-amended peatland. Redundancy analysis identified that the soil water content, soil organic carbon, total nitrogen, and total phosphorus explained the most variance in vegetation composition in the silt-amended peatland. Our results demonstrated that topsoil removal is necessary to reduce the weed seeds and promote the recolonization of peatland species, particularly the tussock-forming Carex, in the silt-amended peatland during restoration.
Does rural livelihood change? Household capital, climate shocks and farm entry-exit decisions in rural Pakistan
Authors: Ahmad Muhammad Irshad , Oxley Les , Ma Hengyun , Liu Ruifeng
Published: January 2023

Agriculture is a primary source of livelihoods in developing countries. The process of entry and exit of farming activities continues to play an important role in maintaining competition in agriculture and allocating resources between agriculture and other sectors. However, climate changes and other economic and social shocks have been severely affecting farmers’ livelihoods. This article examines rural household livelihood transition in the context of farm entry and exit decisions in rural Pakistan. Using 1867 rural household survey data, we brought insights into how livelihood assets, climate shocks, climate investment and locational characteristics affect their farm entry and exit decisions. The results indicate that the proportion of farm entry (24%) was higher than that of farm exit (15%). The major factors were crop inputs using as credit with a huge markup, crop inputs sold by farmers on net cash in financial crisis, climate shocks and poor climate investment that contributed to farm exit. They were household head characteristics, land ownership (family farm), and livestock ownership that increased the likelihood of farm entry decisions. Farm exit decisions were significantly and positively associated with household migration status, irrigation water shortage, off-farm income, crop input used as credit, crop diseases, climate shocks and lack of local government role in sharing climate knowledge. Furthermore, Propensity Score Matching (PSM) results found that the entry decision significantly decreases household income, while the exit decision significantly increases household income and food security status. These findings provide insights into farm entry and exit for those who are planning livelihood transition, and offer recommendations on how to overcome the constraints faced by farming businesses, agricultural sustainability, self-sufficiency and food security during the transition nationally and internationally.
Analysis of urban form typology using urban heat island indicators: Case study of Ferdous neighborhood of Tabriz
Authors: Abdi Zanyar , Alizadeh Hooshmand , Mohammadi Saleh , Sabouri Saber
Published: January 2023

Urbanization, with the acceleration of construction operations in cities, is the main reason for in-creasing the absorption of thermal energy in urban areas, which leads to climate change, especially urban form has responded differently depending on environmental dimension of urban context concerning the urban heat island intensity. In the last Studies, Researchers have not Codified the effect of urban form on heat islands in the form of a comprehensive model. It is necessary to examine each urban context according to its own conditions. Accordingly, this study aims to identify the effect of building form variability on reducing the intensity of heat islands in Ferdous context of Tabriz in the form of comprehensive modelling as a new task. The research gap is the effect of the form of buildings on the intensity of UHI, and the question will be asked whether it is possible to control the intensity of UHI by changing the form by influencing the climatic indicators. Our conceptual framework was based on urban form typologies in microscale affect the amount of energy intake and consumption, especially the intensity of heat islands, and whether the use of desirable types can be responsive to reducing the intensity of these islands? In this regard, using a quantitative analysis method in Rhinoceros software, especially using Grasshopper plugins, urban form is investigated. This analysis includes both the coldest and hottest days of the year. The results show that the optimal model is the cross typology.
High expression of RNF169 is associated with poor prognosis in pancreatic adenocarcinoma by regulating tumour immune infiltration
Authors: Wang Jieyan , Chen Hanghang , Deng Qiong , Chen Yeda , Wang Zhu , Yan Zhengzheng , Wang Yinglin , Tang Haoxuan , Liang Hui , Jiang Yong
Published: January 2023

Background: Pancreatic adenocarcinoma (PAAD) is a highly deadly and aggressive tumour with a poor prognosis. However, the prognostic value of RNF169 and its related mechanisms in PAAD have not been elucidated. In this study, we aimed to explore prognosis-related genes, especially RNF169 in PAAD and to identify novel potential prognostic predictors of PAAD.

Methods: The GEPIA and UALCAN databases were used to investigate the expression and prognostic value of RNF169 in PAAD. The correlation between RNF169 expression and immune infiltration was determined by using TIMER and TISIDB. Correlation analysis with starBase was performed to identify a potential regulatory axis of lncRNA-miRNA-RNF169.

Results: The data showed that the level of RNF169 mRNA expression in PAAD tissues was higher than that in normal tissues. High RNF169 expression was correlated with poor prognosis in PAAD. In addition, analysis with the TISIDB and TIMER databases revealed that RNF169 expression was positively correlated with tumour immune infiltration in PAAD. Correlation analysis suggested that the long non-coding RNA (lncRNA) AL049555.1 and the microRNA (miRNA) hsa-miR-324-5p were involved in the expression of RNF169, composing a potential regulatory axis to control the progression of PAAD. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that RNF169 plays a role in PAAD through pathways such as TNF, Hippo, JAK-STAT and Toll-like receptor signaling.

Conclusion: In summary, the upregulation of RNF169 expression mediated by ncRNAs might influence immune cell infiltration in the microenvironment; thus, it can be used as a prognostic biomarker and a potential therapeutic target in PAAD.
Analysis of genotype × environment interactions for agronomic traits of soybean (Glycine max [L.] Merr.) using association mapping
Authors: Rani Reena , Raza Ghulam , Ashfaq Hamza , Rizwan Muhammad , Shimelis Hussein , Tung Muhammad Haseeb , Arif Muhammad
Published: January 2023

The soybean yield is a complex quantitative trait that is significantly influenced by environmental factors. G × E interaction (GEI), which derives the performance of soybean genotypes differentially in various environmental conditions, is one of the main obstacles to increasing the net production. The primary goal of this study is to identify the outperforming genotypes in different latitudes, which can then be used in future breeding programs. A total of 96 soybean genotypes were examined in two different ecological regions: Faisalabad and Tando Jam in Pakistan. The evaluation of genotypes in different environmental conditions showed a substantial amount of genetic diversity for grain yield. We identified 13 environment-specific genotypes showing their maximum grain yield in each environment. Genotype G69 was found to be an ideal genotype with higher grain yield than other genotypes tested in this study and is broadly adapted for environments E1 and E2 and also included in top-yielding genotypes in E3, E4, and E5. G92 is another genotype that is broadly adapted in E1, E3, and E4. In the case of environments, E3 is suggested to be a more ideal environment as it is plotted near the concentric circle and is very informative for the selection of genotypes with high yield. Despite the presence of GEI, advances in DNA technology provided very useful tools to investigate the insight of advanced genotypes. Association mapping is a useful method for swiftly and efficiently investigating the genetic basis of significant plant traits. A total of 26 marker–trait associations were found for six agronomic traits in five environments, with the highest significance (p-value = 2.48 × 10–08) for plant height and the lowest significance (1.03 × 10–03) for hundred-grain weight. Soybean genotypes identified in the present study could be a valuable source for future breeding programs as they are adaptable to a wide range of environments. Genetic selection of genotypes with the best yields can be used for gross grain production in a wide range of climatic conditions, and it would give an essential reference in terms of soybean variety selection.
COVID-19 inhibits spermatogenesis in the testes by inducing cellular senescence
Authors: Wang Zuomin , Ma Yuxiang , Chen Zude , Yang Riwei , Liu Qinwei , Pan Jinyou , Wang Jiamin , Liu Yangzhou , Zhou Mingda , Zhang Yihan , Zhou Yuhao , Yang Shuxin , Zou Bangyu , Lin Jingwei , Cai Yingxin , Jiang Zheng , Zhou Zhen , Zhao Zhigang
Published: January 2023

Introduction: COVID-19 (SARS-CoV-2) has been linked to organ damage in humans since its worldwide outbreak. It can also induce severe sperm damage, according to research conducted at numerous clinical institutions. However, the exact mechanism of damage is still unknown.

Methods: In this study, testicular bulk-RNA-seq Data were downloaded from three COVID-19 patients and three uninfected controls from GEO to evaluate the effect of COVID-19 infection on spermatogenesis. Relative expression of each pathway and the correlation between genes or pathways were analyzed by bioinformatic methods.

Results: By detecting the relative expression of each pathway and the correlation between genes or pathways, we found that COVID-19 could induce testicular cell senescence through MAPK signaling pathway. Cellular senescence was synergistic with MAPK pathway, which further affected the normal synthesis of cholesterol and androgen, inhibited the normal synthesis of lactate and pyruvate, and ultimately affected spermatogenesis. The medications targeting MAPK signaling pathway, especially MAPK1 and MAPK14, are expected to be effective therapeutic medications for reducing COVID-19 damage to spermatogenesis.

Conclusion: These results give us a new understanding of how COVID-19 inhibits spermatogenesis and provide a possible solution to alleviate this damage.
Standard immunosuppressive treatment reduces regulatory B cells in children with autoimmune liver disease
Authors: Yuksel Muhammed , Nazmi Farinaz , Wardat Dima , Akgül Sebahat , Polat Esra , Akyildiz Murat , Arikan Çigdem
Published: January 2023

Introduction

Autoimmune hepatitis (AIH) is a chronic liver disease caused by a perturbed immune system. The scarcity of short- and long-term immune monitoring of AIH hampered us to comprehend the interaction between immunosuppressive medication and immune homeostasis.

Methods and patients

We recruited children with AIH at the time of diagnosis and at the 1st, 3rd, 6th, 12th, 18th, and 24th months of immunosuppression (IS). We also enrolled children with AIH being on IS for >2 years. Children with drug-induced liver injury (DILI), and those receiving tacrolimus after liver transplantation (LT), were enrolled as disease/IS control subjects. Healthy children (HC) were also recruited. Peripheral blood mononuclear cells (PBMCs) were isolated from all participants. Healthy liver tissue from adult donors and from livers without inflammation were obtained from children with hepatoblastoma. By using flow cytometry, we performed multi-parametric immune profiling of PBMCs and intrahepatic lymphocytes. Additionally, after IS with prednisolone, tacrolimus, rapamycin, or 6-mercaptopurine, we carried out an in vitro cytokine stimulation assay. Finally, a Lifecodes SSO typing kit was used to type HLA-DRB1 and Luminex was used to analyze the results.

Results

Untreated AIH patients had lower total CD8 T-cell frequencies than HC, but these cells were more naïve. While the percentage of naïve regulatory T cells (Tregs) (CD4+FOXP3lowCD45RA+) and regulatory B cells (Bregs, CD20+CD24+CD38+) was similar, AIH patients had fewer activated Tregs (CD4+FOXP3highCD45RA-) compared to HC. Mucosal-associated-invariant-T-cells (MAIT) were also lower in these patients. Following the initiation of IS, the immune profiles demonstrated fluctuations. Bregs frequency decreased substantially at 1 month and did not recover anymore. Additionally, the frequency of intrahepatic Bregs in treated AIH patients was lower, compared to control livers, DILI, and LT patients. Following in vitro IS drugs incubation, only the frequency of IL-10-producing total B-cells increased with tacrolimus and 6MP. Lastly, 70% of AIH patients possessed HLA-DR11, whereas HLA-DR03/DR07/DR13 was present in only some patients.

Conclusion

HLA-DR11 was prominent in our AIH cohort. Activated Tregs and MAIT cell frequencies were lower before IS. Importantly, we discovered a previously unrecognized and long-lasting Bregs scarcity in AIH patients after IS. Tacrolimus and 6MP increased IL-10+ B-cells in vitro.
Disordered T cell-B cell interactions in autoantibody-positive inflammatory arthritis
Authors: Julé Amélie M. , Lam Ki Pui , Taylor Maria , Hoyt Kacie J. , Wei Kevin , Gutierrez-Arcelus Maria , Case Siobhan M. , Chandler Mia , Chang Margaret H. , Cohen Ezra M. , Dedeoglu Fatma , Halyabar Olha , Hausmann Jonathan , Hazen Melissa M. , Janssen Erin , Lo Jeffrey , Lo Mindy S. , Meidan Esra , Roberts Jordan E. , Wobma Holly , Son Mary Beth F. , Sundel Robert P. , Lee Pui Y. , Sage Peter T. , Chatila Talal A. , Nigrovic Peter A. , Rao Deepak A. , Henderson Lauren A.
Published: January 2023

T peripheral helper (Tph) cells, identified in the synovium of adults with seropositive rheumatoid arthritis, drive B cell maturation and antibody production in non-lymphoid tissues. We sought to determine if similarly dysregulated T cell-B cell interactions underlie another form of inflammatory arthritis, juvenile oligoarthritis (oligo JIA). Clonally expanded Tph cells able to promote B cell antibody production preferentially accumulated in the synovial fluid (SF) of oligo JIA patients with antinuclear antibodies (ANA) compared to autoantibody-negative patients. Single-cell transcriptomics enabled further definition of the Tph gene signature in inflamed tissues and showed that Tph cells from ANA-positive patients upregulated genes associated with B cell help to a greater extent than patients without autoantibodies. T cells that co-expressed regulatory T and B cell-help factors were identified. The phenotype of these Tph-like Treg cells suggests an ability to restrain T cell-B cell interactions in tissues. Our findings support the central role of disordered T cell-help to B cells in autoantibody-positive arthritides.
The role of immune cells and associated immunological factors in the immune response to spinal cord injury
Authors: Tang Huaguo , Gu Yuanjie , Jiang Lei , Zheng Gang , Pan Zhuoer , Jiang Xiugui
Published: January 2023

Spinal cord injury (SCI) is a devastating neurological condition prevalent worldwide. Where the pathological mechanisms underlying SCI are concerned, we can distinguish between primary injury caused by initial mechanical damage and secondary injury characterized by a series of biological responses, such as vascular dysfunction, oxidative stress, neurotransmitter toxicity, lipid peroxidation, and immune-inflammatory response. Secondary injury causes further tissue loss and dysfunction, and the immune response appears to be the key molecular mechanism affecting injured tissue regeneration and functional recovery from SCI. Immune response after SCI involves the activation of different immune cells and the production of immunity-associated chemicals. With the development of new biological technologies, such as transcriptomics, the heterogeneity of immune cells and chemicals can be classified with greater precision. In this review, we focus on the current understanding of the heterogeneity of these immune components and the roles they play in SCI, including reactive astrogliosis and glial scar formation, neutrophil migration, macrophage transformation, resident microglia activation and proliferation, and the humoral immunity mediated by T and B cells. We also summarize findings from clinical trials of immunomodulatory therapies for SCI and briefly review promising therapeutic drugs currently being researched.
Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation
Authors: Liu Xu , He Guolin , Lan Yue , Guo Weijie , Liu Xuyuan , Li Jing , Liu Anqing , He Miao , Liu Xinhui , Fan Zhenxin , Zhang Yaoyao
Published: January 2023

Studies have shown that fetal immune cell activation may result from potential exposure to microbes, although the presence of microbes in fetus has been a controversial topic. Here, we combined metagenomic and virome techniques to investigate the presence of bacteria and viruses in fetal tissues (small intestine, cecum, and rectum). We found that the fetal gut is not a sterile environment and has a low abundance but metabolically rich microbiome. Specifically, Proteobacteria and Actinobacteria were the dominant bacteria phyla of fetal gut. In total, 700 species viruses were detected, and Human betaherpesvirus 5 was the most abundant eukaryotic viruses. Especially, we first identified Methanobrevibacter smithii in fetal gut. Through the comparison with adults’ gut microbiota we found that Firmicutes and Bacteroidetes gradually became the main force of gut microbiota during the process of growth and development. Interestingly, 6 antibiotic resistance genes were shared by the fetus and adults. Our results indicate the presence of microbes in the fetal gut and demonstrate the diversity of bacteria, archaea and viruses, which provide support for the studies related to early fetal immunity. This study further explores the specific composition of viruses in the fetal gut and the similarities between fetal and adults’ gut microbiota, which is valuable for understanding human fetal immunity development during gestation.
The emerging role of Th1 cells in atherosclerosis and its implications for therapy
Authors: Chen Jiaojiao , Xiang Xuying , Nie Lei , Guo Xiaoqing , Zhang Feng , Wen Cheng , Xia Yuanpeng , Mao Ling
Published: January 2023

Atherosclerosis is a chronic progressive inflammatory disease of the large and medium-sized artery walls. The molecular mechanisms regulating the onset and progression of atherosclerosis remain unclear. T cells, one of the most common immune cell types in atherosclerotic plaques, are increasingly recognized as a key mediator in the pathogenesis of atherosclerosis. Th1 cells are a subset of CD4+ T helper cells of the adaptive immune system, characterized by the expression of the transcription factor T-bet and secretion of cytokines such as IFN-γ. Converging evidence shows that Th1 cells play a key role in the onset and progression of atherosclerosis. Besides, Th1 is the central mediator to orchestrate the adaptive immune system. In this review, we aim to summarize the complex role of Th1 cells in atherosclerosis and propose novel preventative and therapeutic approaches targeting Th1 cell-associated specific cytokines and receptors to prevent atherogenesis.
Lisinopril prevents bullous pemphigoid induced by dipeptidyl peptidase 4 inhibitors via the Mas receptor pathway
Authors: Nozawa Keisuke , Suzuki Takahide , Kayanuma Gen , Yamamoto Hiroki , Nagayasu Kazuki , Shirakawa Hisashi , Kaneko Shuji
Published: January 2023

Recent studies have suggested that dipeptidyl peptidase 4 (DPP4) inhibitors increase the risk of development of bullous pemphigoid (BP), which is the most common autoimmune blistering skin disease; however, the associated mechanisms remain unclear, and thus far, no therapeutic targets responsible for drug-induced BP have been identified. Therefore, we used clinical data mining to identify candidate drugs that can suppress DPP4 inhibitor-associated BP, and we experimentally examined the underlying molecular mechanisms using human peripheral blood mononuclear cells (hPBMCs). A search of the US Food and Drug Administration Adverse Event Reporting System and the IBM® MarketScan® Research databases indicated that DPP4 inhibitors increased the risk of BP, and that the concomitant use of lisinopril, an angiotensin-converting enzyme inhibitor, significantly decreased the incidence of BP in patients receiving DPP4 inhibitors. Additionally, in vitro experiments with hPBMCs showed that DPP4 inhibitors upregulated mRNA expression of MMP9 and ACE2, which are responsible for the pathophysiology of BP in monocytes/macrophages. Furthermore, lisinopril and Mas receptor (MasR) inhibitors suppressed DPP4 inhibitor-induced upregulation of MMP9. These findings suggest that the modulation of the renin-angiotensin system, especially the angiotensin1-7/MasR axis, is a therapeutic target in DPP4 inhibitor-associated BP.
Serum IL-5 levels predict HBsAg seroclearance in patients treated with Nucleos(t)ide analogues combined with pegylated interferon
Authors: Wang Peipei , Mo Zhishuo , Zhang Ying , Guo Chunxia , Chikede Trevor Kudzai , Chen Dabiao , Lei Ziying , Gao Zhiliang , Zhang Qian , Tong Qiaoxia
Published: January 2023

Background

Knowing about cytokine profile contributes to clarify the underling immune mechanism of HBsAg seroclearance rate increase. This study aims to investigate cytokine changes during nucleos(t)ide analogues (NAs) and peginterferon-α (Peg-IFNα) therapy and their impact on the HBsAg serologic response.

Methods

A total of 78 HBV DNA-negative chronic Hepatitis B (CHB) patients were studied after a lead-in phase of NAs with complete serum cytokines. Serum cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-17 and TNF-α) were quantified by flow cytometry (FCM) every 24 weeks, before, during and at the end of NAs and Peg-IFNα treatment. Clinical and laboratory data were also taken at the same time. Analysis was performed between cured and uncured groups characterized by HBsAg seroclearance. PBMCs samples from five patients (two in cured group and three in uncured group) were analyzed by FCM.

Results

HBsAg seroclearance was achieved in 30 (38,5%) patients defined as the cured group. In comparison to uncured individuals, cured patients showed similar expressions of serum IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17 and TNF-α during the treatment of NAs and Peg-IFNα. Compared with the uncured groups, IL-5 was remarkably increased in cured patients. IL-5 at weeks 24 and 48 were associated with HBsAg seroconversion (p=0.033 and 0.027, respectively). PBMCs sample analysis confirmed the predicted value of IL-5 in response to NAs and Peg-IFNα treatment.

Conclusions

IL-5 at weeks 24 and 48 might be used as a biomarker for HBsAg seroclearance in NAs-experienced CHB patients treated with NAs combined with Peg-IFNα. More importantly, exploiting the expression of this cytokine may help to develop a better understanding of the immune pathogenesis of chronic HBV infection.
The roles of long noncoding RNA-mediated macrophage polarization in respiratory diseases
Authors: Qiao Xin , Ding Yuxiao , Wu Dasen , Zhang Anle , Yin Yan , Wang Qiuyue , Wang Wei , Kang Jian
Published: January 2023

Macrophages play an essential role in maintaining the normal function of the innate and adaptive immune responses during host defence. Macrophages acquire diverse functional phenotypes in response to various microenvironmental stimuli, and are mainly classified into classically activated macrophages (M1) and alternatively activated macrophages (M2). Macrophage polarization participates in the inflammatory, fibrotic, and oncogenic processes of diverse respiratory diseases by changing phenotype and function. In recent decades, with the advent of broad-range profiling methods such as microarrays and next-generation sequencing, the discovery of RNA transcripts that do not encode proteins termed “noncoding RNAs (ncRNAs)” has become more easily accessible. As one major member of the regulatory ncRNA family, long noncoding RNAs (lncRNAs, transcripts >200 nucleotides) participate in multiple pathophysiological processes, including cell proliferation, differentiation, and apoptosis, and vary with different stimulants and cell types. Emerging evidence suggests that lncRNAs account for the regulation of macrophage polarization and subsequent effects on respiratory diseases. In this review, we summarize the current published literature from the PubMed database concerning lncRNAs relevant to macrophage polarization and the underlying molecular mechanisms during the occurrence and development of respiratory diseases. These differentially expressed lncRNAs are expected to be biomarkers and targets for the therapeutic regulation of macrophage polarization during disease development.
Study on pathological and clinical characteristics of chronic HBV infected patients with HBsAg positive, HBV DNA negative, HBeAg negative
Authors: Zeng Zhan , Liu Ruyu , Cao Weihua , Yang Liu , Lin Yanjie , Bi Xiaoyue , Jiang Tingting , Deng Wen , Wang Shiyu , Lu Huihui , Sun Fangfang , Shen Ge , Chang Min , Lu Yao , Wu Shuling , Hao Hongxiao , Xu Mengjiao , Chen Xiaoxue , Hu Leiping , Zhang Lu , Wan Gang , Xie Yao , Li Minghui
Published: January 2023

Aims

Study of clinical characteristics of hepatitis B virus deoxyribonucleic acid (HBV DNA)-negative, hepatitis B surface antigen (HBsAg)-positive, hepatitis B e antigen (HBeAg)-negative patients based on liver histopathology.

Methods

We retrospectively enrolled patients with chronic HBV infection diagnosis at Beijing Ditan Hospital from May 2008 to November 2020. To study the differences between patients with significant hepatic histopathology and those without significant hepatic histopathology. And to study the independent factors of significant hepatic histopathology.

Results

85 HBV DNA-negative and HBeAg-negative patients were 37.90 ± 10.30 years old, 23.50% of patients with grade of inflammation (G) >1, 35.30% of patients with liver fibrosis stage (S) >1, 44.70% patients were diagnosed with significant hepatic histopathology. Compared to the no significant hepatic histopathology group, another group had older age (41.70 ± 10.70 vs 34.80 ± 8.87 years, t=-3.28, P=0.002), higher total bilirubin (TBIL) [14.9(10.3, 22.4) vs 11(8.9, 14.4) μmol/L, z=-2.26, P=0.024], lower cholinesterase (CHE) (t=-2.86, P=0.005, 7388.00 ± 2156.00 vs 8988.00 ± 2823.00 U/L) and lower platelet (PLT) (t=2.75, P=0.007, 157.00 ± 61.40 vs 194.00 ± 61.00 10^9/L). Abnormal ALT patients are more likely to have significant hepatic histopathology (z=5.44, P=0.020, 66.70% vs 337.50%). G had significant correlation with CHE (P=0.008, r=-0.23), alanine aminotransferase (ALT) (P=0.041, r=0.18), aspartate aminotransferase (AST) (P=0.001, r=0.29). S had significant correlation with TBIL (P = 0.008, r = 0.23), age (P < 0.001, r = 0.32), international normalized ratio (INR) (P = 0.04, r = 0.23), CHE (P < 0.001, r = -0.30), PLT (P < 0.001, r = -0.40) and prothrombin time activity (PTA) (P = 0.046, r = -0.22). Multivariate logistic analysis indicated only age (95%CI=1.014~1.130, OR=1.069, P=0.013) was an impact factor for significant hepatic histopathology. The cutoff point of age was 34.30 years.

Conclusions

A large proportion of chronic HBV infection patients with HBeAg-negative and HBV DNA-negative still have chronic hepatitis. Age is an independent factor for significant hepatic histopathology
Routine laboratory parameters predict intensive care unit admission and hospitalization in patients suffering stab injuries
Authors: Maleitzke Tazio , Zhou Sijia , Zocholl Dario , Fleckenstein Florian Nima , Back David Alexander , Plewe Julius Maximilian , Weber Jérôme , Winkler Tobias , Stöckle Ulrich , Tsitsilonis Serafeim , Märdian Sven
Published: January 2023

Background

Knife crime has increased considerably in recent years in Northern Europe. Affected patients often require immediate surgical care due to traumatic organ injury. Yet, little is known about clinically relevant routine laboratory parameters in stab injury patients and how these are associated with intensive care unit (ICU) admission, hospitalization and number of surgeries.

Methods

We retrospectively analyzed 258 stab injury cases between July 2015 and December 2021 at an urban Level I Trauma Center. Annual and seasonal incidences, injury site, injury mechanism, Injury Severity Score (ISS), and surgical management were evaluated. First, correlations between routine laboratory parameters for hematology, coagulation, and serum biochemistry (peak, and Δ (change from admission to peak within 3 days following admission)) and length of hospital stay, ICU stay, and number of surgeries were assessed using Spearman’s rank correlation coefficients. Second, multivariable Least Absolute Shrinkage and Selection Operator (LASSO) regression analyses were conducted to identify parameters predictive of clinical outcomes. Third, longitudinal developments of routine laboratory parameters were assessed during hospital admission.

Results

In 2021, significantly more stab injuries were recorded compared with previous years and occurred less during winter compared with other seasons. Mean ISS was 8.3 ± 7.3, and ISS was positively correlated with length of hospital and ICU stay (r = 0.5–0.8, p < 0.001). Aspartate transaminase (AST) (Δ) (r = 0.690), peak C-reactive protein (CrP) (r = 0.573), and erythrocyte count (Δ) (r = 0.526) showed the strongest positive correlations for length of ICU stay for penetrating, thoracoabdominal, and organ injuries, respectively. No correlations were observed between routine laboratory parameters and number of surgeries. For patients with penetrating injuries, LASSO-selected predictors of ICU admission included ISS, pH and lactate at admission, and Δ values for activated partial thromboplastin time (aPTT), K+, and erythrocyte count. CrP levels on day 3 were significantly higher in patients with penetrating (p = 0.005), thoracoabdominal (p = 0.041), and organ injuries (p < 0.001) compared with those without.

Conclusion

Our data demonstrate an increase in stab injury cases in 2021 and an important link between changes in routine laboratory parameters and ICU admission and hospitalization. Monitoring ISS and changes in AST, CrP, erythrocyte count, pH, lactate, aPTT, and K+ may be useful to identify patients at risk and adjust surgical and ICU algorithms early on.
The 2022 Diadema antillarum die-off event: Comparisons with the 1983-1984 mass mortality
Authors: Hylkema Alwin , Kitson-Walters Kimani , Kramer Patricia R. , Patterson Joshua T. , Roth Lynnette , Sevier Moriah L. B. , Vega-Rodriguez Maria , Warham Matthew M. , Williams Stacey M. , Lang Judith C.
Published: January 2023

The 1983-1984 die-off of the long-spined sea urchin Diadema antillarum stands out as a catastrophic marine event because of its detrimental effects on Caribbean coral reefs. Without the grazing activities of this key herbivore, turf and macroalgae became the dominant benthic group, inhibiting coral recruitment and compromising coral reef recovery from other disturbances. In the decades that followed, recovery of D. antillarum populations was slow to non-existent. In late January 2022, a new mass mortality of D. antillarum was first observed in the U.S. Virgin Islands. We documented the spread and extent of this new die-off using an online survey. Infected individuals were closely monitored in the lab to record signs of illness, while a large population on Saba, Dutch Caribbean, was surveyed weekly before and during mortality to determine the lethality of this event. Within four months the die-off was distributed over 1,300 km from north to south and 2,500 km east to west. Whereas the 1983-1984 die-off advanced mostly with the currents, the 2022 event has appeared far more quickly in geographically distant areas. First die-off observations in each jurisdiction were often close to harbor areas, which, together with their rapid appearance, suggests that anthropogenic factors may have contributed to the spread of the causative agent. The signs of illness in sick D. antillarum were very similar to those recorded during the 1983-1984 die-off: lack of tube feet control, slow spine reaction followed by their loss, and necrosis of the epidermis were observed in both lab and wild urchins. Affected populations succumbed fast; within a month of the first signs of illness, a closely monitored population at Saba, Dutch Caribbean, had decreased from 4.05 individuals per m2 to 0.05 individuals per m2. Lethality can therefore be as high as 99%. The full extent of the 2022 D. antillarum die-off event is not currently known. The slower spread in the summer of 2022 might indicate that the die-off is coming to a (temporary) standstill. If this is the case, some populations will remain unaffected and potentially supply larvae to downstream areas and augment natural recovery processes. In addition, several D. antillarum rehabilitation approaches have been developed in the past decade and some are ready for large scale implementation. However, active conservation and restoration should not distract from the primary goal of identifying a cause and, if possible, implementing actions to decrease the likelihood of future D. antillarum die-off events.
Two near-inertial peaks in antiphase controlled by stratification and tides in the Yellow Sea
Authors: Hu Yibo , Yu Fei , Chen Zifei , Si Guangcheng , Liu Xingchuan , Nan Feng , Ren Qiang
Published: January 2023

The Yellow Sea is a strongly tidally-driven and highly stratified shallow sea due to the presence of the Yellow Sea Cold Water Masses. Observations show that the near-inertial event sustains for 10 days with a peak near-inertial velocity of 0.15m/s, which accounts for 30% of the total velocity during the passage of a cyclone. Near-inertial velocity is dominated by the first baroclinic mode with one zero-crossing at the depth of the maximum stratification and two velocity peaks in the mixed layer and below the thermocline, respectively. Combined with numerical simulation analysis, it was found that the two velocity peaks are controlled by stratification and tides. In the mixed layer, the near-inertial peak is induced by wind stress, but the strong stratification constrains the downward propagation of the near-inertial energy. With respect to the near-inertial peak below the thermocline, it is associated with a barotropic wave generated at the coast and propagating offshore. However, the near-inertial flow within the bottom layer is reduced by the eddy viscosity of the tidal currents. Within the thermocline, the pronounced vertical convection due to velocity shear weakens the intensity of the near-inertial flow.
Performance characteristics of 18F–fluorodeoxyglucose in non-infected hip replacement
Authors: Wang Yiqun , Yuan Lulu , Du Yinqiao , Liu Honghong , Li Qingxiao , Chang Yan , Shi Yuanyuan , Wang Yanmei , Meng Xiaolin , Zhou Yonggang , Yao Shulin , Tian Jiahe
Published: January 2023

Purpose

The aim of this study was to retrospectively analyze 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET)/ computed tomography (CT) images of non-infected hip arthroplasty patients and summarize findings that may be useful for clinical practice.

Methods

18F-FDG PET/CT images of non-infected hip arthroplasty patients were collected from September 2009 to August 2021. The region of interest was independently delineated by two physicians and maximum standardized uptake values (SUVmax) were recorded and compared. Serologic data were also collected and the correlation between SUVmax and serologic parameters was analyzed, while the images were classified based on the 18F-FDG uptake pattern in the images using the diagnostic criteria proposed by Reinartz et al. (9). The interval between hip replacement and PET/CT was classified by year and the characteristics of the two groups were compared. The images of patients who underwent PET/CT multiple times were analyzed dynamically.

Results

A total of 121 examinations were included; six patients underwent PET/CT twice and two patients had three scans. There were no significant correlations between SUVmax and serologic results. The interobserver agreement between the two physicians in the classification according to the criteria of Reinartz et al. (9) was 0.957 (P < 0.005). Although there was non-specific uptake in cases with an arthroplasty-to-PET/CT interval this was non-significant. Additionally, 18F-FDG showed potential utility for dynamic observation of the condition of the hip.

Conclusion

SUVmax provided information independent of serologic results, meanwhile 18F-FDG showed potential applicability to the dynamic monitoring of hip arthroplasty-related diseases. However, the presence of blood vessels and muscles affected image interpretation and the specificity of 18F-FDG was not optimal. A more specific radionuclide is needed to maximize the benefits of using PET/CT for the assessment of periprosthetic joint infection (PJI).
Methods on LDL particle isolation, characterization, and component fractionation for the development of novel specific oxidized LDL status markers for atherosclerotic disease risk assessment
Authors: Papadea Polyxeni , Skipitari Marianna , Kalaitzopoulou Electra , Varemmenou Athina , Spiliopoulou Maria , Papasotiriou Marios , Papachristou Evangelos , Goumenos Dimitrios , Onoufriou Anny , Rosmaraki Eleftheria , Margiolaki Irene , Georgiou Christos D.
Published: January 2023

The present study uses simple, innovative methods to isolate, characterize and fractionate LDL in its main components for the study of specific oxidations on them that characterize oxidized low-density lipoprotein (oxLDL) status, as it causatively relates to atherosclerosis-associated cardiovascular disease (CVD) risk assessment. These methods are: (a) A simple, relatively time-short, low cost protocol for LDL isolation, to avoid shortcomings of the currently employed ultracentrifugation and affinity chromatography methodologies. (b) LDL purity verification by apoB100 SDS-PAGE analysis and by LDL particle size determination; the latter and its serum concentration are determined in the present study by a simple method more clinically feasible as marker of CVD risk assessment than nuclear magnetic resonance. (c) A protocol for LDL fractionation, for the first time, into its main protein/lipid components (apoB100, phospholipids, triglycerides, free cholesterol, and cholesteryl esters), as well as into LDL carotenoid/tocopherol content. (d) Protocols for the measurement, for the first time, of indicative specific LDL component oxidative modifications (cholesteryl ester-OOH, triglyceride-OOH, free cholesterol-OOH, phospholipid-OOH, apoB100-MDA, and apoB100-DiTyr) out of the many (known/unknown/under development) that collectively define oxLDL status, which contrasts with the current non-specific oxLDL status evaluation methods. The indicative oxLDL status markers, selected in the present study on the basis of expressing early oxidative stress-induced oxidative effects on LDL, are studied for the first time on patients with end stage kidney disease on maintenance hemodialysis, selected as an indicative model for atherosclerosis associated diseases. Isolating LDL and fractionating its protein and main lipid components, as well as its antioxidant arsenal comprised of carotenoids and tocopherols, paves the way for future studies to investigate all possible oxidative modifications responsible for turning LDL to oxLDL in association to their possible escaping from LDL’s internal antioxidant defense. This can lead to studies to identify those oxidative modifications of oxLDL (after their artificial generation on LDL), which are recognized by macrophages and convert them to foam cells, known to be responsible for the formation of atherosclerotic plaques that lead to the various CVDs.
Preclinical models derived from endoscopic ultrasound-guided tissue acquisition for individualized treatment of pancreatic ductal adenocarcinoma
Authors: Tong Ting , Zhang Chao , Li Jingbo , Deng Minzi , Wang Xiaoyan
Published: January 2023

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with poor outcomes. Although the management strategies have evolved in recent years, the PDAC 5-year survival rate remains at only 9%; it may become the second leading cause of cancer death in the USA by 2030. Only 15–20% of PDAC patients are eligible to undergo surgery; diagnostic biopsies and individualized treatment present a more significant challenge for the remaining group. Endoscopic ultrasound-guided tissue acquisition (EUS-TA) has been widely used in the diagnosis of pancreatic masses. With the advancement of this sampling technique, adequate specimens can be obtained from all patients with PDAC in both early and late clinical stages. Recent data suggest that the specimens obtained from EUS-TA might be used to establish viable preclinical models, which conserve the genetic mutation and preserve the heterogeneity of the original tumors. Additionally, any drug sensitivity evident in the EUS-TA-derived preclinical models might predict the clinical response, thus guiding the prospective therapeutic selection. As we move toward the era of precision medicine, this review provides an update on the role of EUS-TA as a method for obtaining genetic material used in preclinical models that can assess and stratify individuals according to their individual cancer biology.
Clinical applications of antimicrobial photodynamic therapy in dentistry
Authors: Gholami Leila , Shahabi Shiva , Jazaeri Marzieh , Hadilou Mahdi , Fekrazad Reza
Published: January 2023

Given the emergence of resistant bacterial strains and novel microorganisms that globally threaten human life, moving toward new treatment modalities for microbial infections has become a priority more than ever. Antimicrobial photodynamic therapy (aPDT) has been introduced as a promising and non-invasive local and adjuvant treatment in several oral infectious diseases. Its efficacy for elimination of bacterial, fungal, and viral infections and key pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Candida albicans, and Enterococcus faecalis have been investigated by many invitro and clinical studies. Researchers have also investigated methods of increasing the efficacy of such treatment modalities by amazing developments in the production of natural, nano based, and targeted photosensitizers. As clinical studies have an important role in paving the way towards evidence-based applications in oral infection treatment by this method, the current review aimed to provide an overall view of potential clinical applications in this field and summarize the data of available randomized controlled clinical studies conducted on the applications of aPDT in dentistry and investigate its future horizons in the dental practice. Four databases including PubMed (Medline), Web of Science, Scopus and Embase were searched up to September 2022 to retrieve related clinical studies. There are several clinical studies reporting aPDT as an effective adjunctive treatment modality capable of reducing pathogenic bacterial loads in periodontal and peri-implant, and persistent endodontic infections. Clinical evidence also reveals a therapeutic potential for aPDT in prevention and reduction of cariogenic organisms and treatment of infections with fungal or viral origins, however, the number of randomized clinical studies in these groups are much less. Altogether, various photosensitizers have been used and it is still not possible to recommend specific irradiation parameters due to heterogenicity among studies. Reaching effective clinical protocols and parameters of this treatment is difficult and requires further high quality randomized controlled trials focusing on specific PS and irradiation parameters that have shown to have clinical efficacy and are able to reduce pathogenic bacterial loads with sufficient follow-up periods.