bluuebunny/crossref_metadata_2025 huggingface dataset:
The Management of Myocardial Injury Related to SARS-CoV-2 Pneumonia
Authors: Akkaif Mohammed Ahmed , Bitar Ahmad Naoras , Al-Kaif Laith A. I. K. , Daud Nur Aizati Athirah , Sha’aban Abubakar , Noor Dzul Azri Mohamed , Abd Aziz Fatimatuzzahra’ , Cesaro Arturo , SK Abdul Kader Muhamad Ali , Abdul Wahab Mohamed Jahangir , Khaw Chee Sin , Ibrahim Baharudin
Published: September 2022

The global evolution of the SARS-CoV-2 virus is known to all. The diagnosis of SARS-CoV-2 pneumonia is expected to worsen, and mortality will be higher when combined with myocardial injury (MI). The combination of novel coronavirus infections in patients with MI can cause confusion in diagnosis and assessment, with each condition exacerbating the other, and increasing the complexity and difficulty of treatment. It would be a formidable challenge for clinical practice to deal with this situation. Therefore, this review aims to gather literature on the progress in managing MI related to SARS-CoV-2 pneumonia. This article reviews the definition, pathogenesis, clinical evaluation, management, and treatment plan for MI related to SARS-CoV-2 pneumonia based on the most recent literature, diagnosis, and treatment trial reports. Many studies have shown that early diagnosis and implementation of targeted treatment measures according to the different stages of disease can reduce the mortality rate among patients with MI related to SARS-CoV-2 pneumonia. The reviewed studies show that multiple strategies have been adopted for the management of MI related to COVID-19. Clinicians should closely monitor SARS-CoV-2 pneumonia patients with MI, as their condition can rapidly deteriorate and progress to heart failure, acute myocardial infarction, and/or cardiogenic shock. In addition, appropriate measures need to be implemented in the diagnosis and treatment to provide reasonable care to the patient.
Downregulation of Mannose-6-Phosphate Receptors in Fabry Disease Cardiomyopathy: A Potential Target for Enzyme Therapy Enhancement
Authors: Frustaci Andrea , Verardo Romina , Scialla Rossella , Bagnato Giulia , Verardo Margherita , Alfarano Maria , Russo Matteo
Published: September 2022

Background: The efficacy of enzyme replacement therapy (ERT) in mobilizing globotryaosylceramide (GB-3) from Fabry cardiomyocytes is limited. The mechanism involved is still obscure. Methods: Assessment of M6Pr, M6Pr-mRNA, and Ubiquitin has been obtained by Western blot analysis and real-time PCR of frozen endomyocardial biopsy samples, from 17 pts with FD, various degree of left ventricular hypertrophy, and maximal wall thickening (MWT) from 11.5 and 20 mm. The diagnosis and severity of FDCM followed definitions of GLA mutation, α-galactosidase A enzyme activity, cardiac magnetic resonance, and left ventricular endomyocardial biopsy with the quantification of myocyte hypertrophy and the extent of Gb-3 accumulation. All patients have received alpha or beta agalsidase for ≥3 years without a reduction in LV mass nor an increase in T1 mapping at CMR. Controls were surgical biopsies from 15 patients undergoing mitral valve replacement. Results: Protein analysis showed mean M6Pr in FDCM to be 5.4-fold lower than in a normal heart (4289 ± 6595 vs. 23,581 ± 4074, p = 0.0996) (p < 0.001): specifically, 9-fold lower in males, p = 0.009, (p < 0.001) and 3-fold lower in females, p = 0.5799, (p < 0.001) showing, at histology, a mosaic of normal and diseased cells. M6Pr-mRNA expression was normal, while ubiquitin showed an increase of 4.6 fold vs. controls (13,284 ± 1723 vs. 2870 ± 690, p = 0.001) suggesting that ubiquitin-dependent post-translational degradation is likely responsible for the reduction of M6Pr in FDCM. Conclusion: M6Pr expression is remarkably reduced in FDCM as a likely result of post-translational degradation. This may explain the reduced efficacy of ERT and be a therapeutic target for the enhancement of ERT activity.
Multimorbidity and Frailty Are the Key Characteristics of Patients Hospitalized with COVID-19 Breakthrough Infection during Delta Variant Predominance in Italy: A Retrospective Study
Authors: Ticinesi Andrea , Parise Alberto , Cerundolo Nicoletta , Nouvenne Antonio , Prati Beatrice , Chiussi Giulia , Guerra Angela , Meschi Tiziana
Published: September 2022

The aims of this study were to describe the characteristics of patients hospitalized with delta SARS-CoV-2 breakthrough infection, and to identify factors associated with pneumonia on chest Computed Tomography (CT) and mortality. The clinical records of 229 patients (105 F), with a median age of 81 (interquartile range, IQR, 73–88) years old, hospitalized between June and December 2021 after completion of the primary vaccination cycle, were retrospectively analyzed, retrieving data on comorbidities, Clinical Frailty Scale (CFS), clinical presentation and outcomes. Multimorbidity (91.7% with ≥2 chronic illnesses) and frailty (61.6% with CFS ≥ 5) were highly prevalent. CFS (OR 0.678, 95% CI 0.573–0.803, p < 0.001) and hypertension were independently associated with interstitial pneumonia. Mortality was 25.1% and unrelated with age. PaO2/FiO2 on blood gas analysis performed upon admission (OR 0.986, 95% CI 0.977–0.996, p = 0.005), and CFS (OR 1.723, 95% CI 1.152–2.576, p = 0.008) were independently associated with mortality only in subjects < 85 years old. Conversely, serum PCT levels were associated with mortality in subjects ≥ 85 years old (OR 3.088, 95% CI 1.389–6.8628, p = 0.006). In conclusion, hospitalization for COVID-19 breakthrough infection mainly involved geriatric patients, with those aged ≥ 85 more characterized by decompensation of baseline comorbidities rather than typical COVID-19 respiratory symptoms.
Evaluation of a Triage Checklist for Mild COVID-19 Outpatients in Predicting Subsequent Emergency Department Visits and Hospitalization during the Isolation Period: A Single-Center Retrospective Study
Authors: Tanaka Yasuhiro , Yamamoto Kazuko , Morimoto Shimpei , Nabeshima Takeshi , Matsushima Kayoko , Ishimoto Hiroshi , Ashizawa Nobuyuki , Hirayama Tatsuro , Takeda Kazuaki , Gyotoku Hiroshi , Iwanaga Naoki , Takemoto Shinnosuke , Fukahori Susumu , Takazono Takahiro , Yamaguchi Hiroyuki , Kido Takashi , Sakamoto Noriho , Hosogaya Naoki , Akabame Shogo , Sugimoto Takashi , Yamanashi Hirotomo , Matsui Kosuke , Izumida Mai , Fujita Ayumi , Tashiro Masato , Tanaka Takeshi , Ariyoshi Koya , Furumoto Akitsugu , Morita Kouichi , Izumikawa Koichi , Yanagihara Katsunori , Mukae Hiroshi
Published: September 2022

Managing mild illness in COVID-19 and predicting progression to severe disease are concerning issues. Here, we investigated the outcomes of Japanese patients with mild COVID-19, and identified triage risk factors for further hospitalization and emergency department (ED) visits at a single tertiary hospital. A triage checklist with 30 factors was used. Patients recommended for isolation were followed up for 10 days for subsequent ED visits or hospital admission. Overall, 338 patients (median age, 44.0; 45% women) visited the clinic 5.0 days (median) after symptom onset. Thirty-six patients were immediately hospitalized following triage; others were isolated. In total, 72 non-hospitalized patients visited the ED during their isolation, and 30 were hospitalized after evaluation for oxygen desaturation. The median ED visit and hospitalization durations after symptom onset were 5.0 and 8.0 days, respectively. The checklist factors associated with hospitalization during isolation were age > 50 years, body mass index > 25 kg/m2, hypertension, tachycardia with pulse rate > 100/min or blood pressure > 135 mmHg at triage, and >3-day delay in hospital visit after symptom onset. No patients died. Altogether, 80% of patients with mild COVID-19 could be safely isolated at home. Age, BMI, underlying hypertension, date after symptom onset, tachycardia, and systolic blood pressure at triage might be related to later hospitalization.
Relationship between the Levels of Calprotectin and Soluble Receptor for Advanced Glycation End Products with Abdominal Aortic Aneurysm Diameter: A Preliminary Clinical Trial
Authors: Hauzer Willy , Gnus Jan , Rosińczuk Joanna
Published: September 2022

An abdominal aortic aneurysm (AAA) is a dilatation of the abdominal aorta greater than 50% of the diameter of a healthy aorta. Previous experimental studies confirm the effect of calprotectin (CAL) on the onset of arterial pathology. It has been suggested that low levels of soluble receptors for advanced glycation end products (RAGEs) increase levels of cytokines that lead to the inhibition of matrix metalloproteinases (MMPs), affecting AAA formation. This study aimed to analyze the correlation of levels of RAGE and CAL with AAA diameter. A group of 32 patients aged 50–75 with diagnosed AAA was enrolled in the study. This group of patients was further divided into three subgroups based on AAA diameter: (1) <4.5 cm, (2) 4.5–5.5 cm, (3) >5.5 cm. Peripheral blood was drawn from all participants on admission to measure the serum CAL and RAGE levels. An enumeration survey was performed three months after AAA surgical treatment. CAL and RAGE plasma levels were measured with the enzyme-linked immunosorbent assay (ELISA). The median CAL levels were 2273.0 ng/mL before and 1217.0 ng/mL after treatment. There was a statistically significant decrease in CAL levels following the surgical treatment (p = 0.003). The correlation analysis between CAL levels and RAGE levels before and after surgical treatment showed no statistically significant correlations. In addition, there were no statistically significant correlations between CAL and RAGE levels with AAA size. In conclusion, CAL levels appear to be a significant marker in patients with AAA. There is an almost twofold decrease in CAL levels after AAA excision.
Influence of Bone Conduction Hearing Implantation on Health-Related Quality of Life for Patients with Chronic Otitis Media
Authors: Lewis Aaran T. , Gergely Viktor
Published: September 2022

Background: Chronic otitis media is a major public health burden that can result in a disabling hearing loss. Bone conduction hearing implants are an accepted form of hearing rehabilitation in these patients, but evidence supporting their usage typically comes from studies investigating mixed indications. The objective of our study was to examine how these devices impact health-related quality of life and hearing-disability in adult patients suffering from chronic otitis media. Methods: Health Utilities Index-mark III (n = 10) and Speech Spatial and Qualities of Hearing-49 data (n = 6) were extracted for adult patients with chronic otitis media from an international hearing implant registry. Data were compared at baseline and at 12-month post-implantation with a bone conduction hearing implant. Results: Patients demonstrated a clinically relevant mean utility gain of 0.145 following implantation and clinically relevant mean improvement in global speech spatial and qualities of hearing score following implantation. Conclusions: Bone conduction implantation was found to improve hearing and health-related quality of life and reduce hearing disability in a small cohort of patients with chronic otitis media. These data highlight the importance of providing appropriate hearing rehabilitation for individuals with chronic otitis media.
Detection of Lumbar Spondylolisthesis from X-ray Images Using Deep Learning Network
Authors: Trinh Giam Minh , Shao Hao-Chiang , Hsieh Kevin Li-Chun , Lee Ching-Yu , Liu Hsiao-Wei , Lai Chen-Wei , Chou Sen-Yi , Tsai Pei-I , Chen Kuan-Jen , Chang Fang-Chieh , Wu Meng-Huang , Huang Tsung-Jen
Published: September 2022

Spondylolisthesis refers to the displacement of a vertebral body relative to the vertrabra below it, which can cause radicular symptoms, back pain or leg pain. It usually occurs in the lower lumbar spine, especially in women over the age of 60. The prevalence of spondylolisthesis is expected to rise as the global population ages, requiring prudent action to promptly identify it in clinical settings. The goal of this study was to develop a computer-aided diagnostic (CADx) algorithm, LumbarNet, and to evaluate the efficiency of this model in automatically detecting spondylolisthesis from lumbar X-ray images. Built upon U-Net, feature fusion module (FFM) and collaborating with (i) a P-grade, (ii) a piecewise slope detection (PSD) scheme, and (iii) a dynamic shift (DS), LumbarNet was able to analyze complex structural patterns on lumbar X-ray images, including true lateral, flexion, and extension lateral views. Our results showed that the model achieved a mean intersection over union (mIOU) value of 0.88 in vertebral region segmentation and an accuracy of 88.83% in vertebral slip detection. We conclude that LumbarNet outperformed U-Net, a commonly used method in medical image segmentation, and could serve as a reliable method to identify spondylolisthesis.
The Analgesic Efficacy of the Single Erector Spinae Plane Block with Intercostal Nerve Block Is Not Inferior to That of the Thoracic Paravertebral Block with Intercostal Nerve Block in Video-Assisted Thoracic Surgery
Authors: Kim Sujin , Song Seung Woo , Do Hyejin , Hong Jinwon , Byun Chun Sung , Park Ji-Hyoung
Published: September 2022

This monocentric, single-blinded, randomized controlled noninferiority trial investigated the analgesic efficacy of erector spinae plane block (ESPB) combined with intercostal nerve block (ICNB) compared to that of thoracic paravertebral block (PVB) with ICNB in 52 patients undergoing video-assisted thoracic surgery (VATS). The endpoints included the difference in visual analog scale (VAS) scores for pain (0–10, where 10 = worst imaginable pain) in the postanesthetic care unit (PACU) and 24 and 48 h postoperatively between the ESPB and PVB groups. The secondary endpoints included patient satisfaction (1–5, where 5 = extremely satisfied) and total analgesic requirement in morphine milligram equivalents (MME). Median VAS scores were not significantly different between the groups (PACU: 2.0 (1.8, 5.3) vs. 2.0 (2.0, 4.0), p = 0.970; 24 h: 2.0 (0.8, 3.0) vs. 2.0 (1.0, 3.5), p = 0.993; 48 h: 1.0 (0.0, 3.5) vs. 1.0 (0.0, 5.0), p = 0.985). The upper limit of the 95% CI for the differences (PACU: 1.428, 24 h: 1.052, 48 h: 1.176) was within the predefined noninferiority margin of 2. Total doses of rescue analgesics (110.24 ± 103.64 vs. 118.40 ± 93.52 MME, p = 0.767) and satisfaction scores (3.5 (3.0, 4.0) vs. 4.0 (3.0, 5.0), p = 0.227) were similar. Thus, the ESPB combined with ICNB may be an efficacious option after VATS.
A Program to Reduce Post-Operative Opioid Prescribing at a Veteran’s Affairs Hospital
Authors: Hlavin Callie , Muluk Sruthi , Muluk Visala , Ryan John , Wagner Jeffrey , Dhupar Rajeev
Published: September 2022

Variability in surgeon prescribing patterns is common in the post-operative period and can be the nidus for dependence and addiction. This project aims to reduce opioid overprescribing at the Veteran’s Affairs Pittsburgh Healthcare System (VAPHS). The VAPHS Opioid Stewardship Committee collaborated to create prescribing guidelines for inpatient and outpatient general, thoracic, and vascular surgery procedures. We incorporated bundled order sets into the provider workflow in the electronic medical system and performed a retrospective cohort study comparing opioid prescription patterns for Veterans who underwent any surgical procedure for a three-month period pre- and post- guideline implementation. After implementation of opioid prescribing guidelines, morphine milligram equivalents (MME), quantity of pills prescribed, and days prescribed were statistically significantly reduced for procedures with associated guidelines, including cholecystectomy (MME 140.8 vs. 57.5, p = 0.002; quantity 18.8 vs. 8, p = 0.002; days 5.1 vs. 2.8, p = 0.021), inguinal hernia repair (MME 129.9 vs. 45.3, p = 0.002; quantity 17.3 vs. 6.1, p = 0.002; days 5.0 vs. 2.4, p = 0.002), and umbilical hernia repair (MME 128.8 vs. 53.8, p = 0.002; quantity 17.1 vs. 7.8, p = 0.002; days 5.1 vs. 2.5, p = 0.022). Procedures without associated recommendations also preceded a decrease in overall opioid prescribing. Post-operative opioid prescribing guidelines can steer clinicians toward more conscientious opioid disbursement. There may also be reductions in prescribing opioids for procedures without guidelines as an indirect effect of practice change.
Early Determinants of Adverse Motor Outcomes in Preschool Children with a Critical Congenital Heart Defect
Authors: Sprong Maaike C. A. , Huijgen Barbara C. H. , de Vries Linda S. , Talacua Hanna , van Loon Kim , Eijsermans Rian M. J. C. , Nijman Joppe , Breur Johannes M. P. J. , van Brussel Marco , Slieker Martijn G.
Published: September 2022

Neurodevelopmental disabilities are common in infants with critical congenital heart disease (CCHD). A prospective, longitudinal cohort study was conducted to establish the prevalence and early determinants of adverse motor outcomes in infants who underwent cardiac surgery with cardiopulmonary bypass before six months of age. Motor development was assessed in 147 preschoolers using the Movement Assessment Battery for children-II. Although the majority displayed an average motor development, 22% of preschool children with CCHD deteriorated in their motor developmental score compared to their previous assessment at 18 months, especially in those with an aortic arch anomaly (AAA) (35%). Individual stability over time appeared to be moderate and the number of children with a motor delay increased, up to 20% in children with AAA. Motor development up to 42 months was best predicted by gestational age, cardio pulmonary bypass time, aortic cross clamp time, number of heart catheterizations up to 18 months and early motor outcomes. The increase in number of preschool children with a motor delay underlines the importance of longitudinal screening of motor skills in children with CCHD at risk for adverse motor outcomes. Offering early interventions may protect their current and future cardiovascular health as motor development is an independent predictor of exercise capacity, physical activity and participation in daily living.
Alanine-Serine-Cysteine Transporter 2 Inhibition Suppresses Prostate Cancer Cell Growth In Vitro
Authors: Saruta Masanobu , Takahara Kiyoshi , Yoshizawa Atsuhiko , Niimi Atsuko , Takeuchi Toshiyuki , Nukaya Takuhisa , Takenaka Masashi , Zennami Kenji , Ichino Manabu , Sasaki Hitomi , Kusaka Mamoru , Suzuki Motoshi , Sumitomo Makoto , Shiroki Ryoichi
Published: September 2022

Alanine-serine-cysteine transporter 2 (ASCT2) has been associated with increased levels of metabolism in various malignant tumors. However, its biological significance in the proliferation of prostate cancer (PCa) cells remains under investigation. We used the cBioPortal database to assess the effect of ASCT2 expression on the oncological outcomes of 108 PCa patients. To evaluate the function of ASCT2 in castration-sensitive PCa (CSPC) and castration-resistant PCa (CRPC), LNCaP cells and the ARV7-positive PCa cell line, 22Rv1, were assessed using cell proliferation assays and Western blot analyses. The ASCT2 expression level was associated with biochemical recurrence-free survival after prostatectomy in patients with a Gleason score ≥ 7. In vitro experiments indicated that the growth of LNCaP cells after combination therapy of ASCT2 siRNA and enzalutamide treatment was significantly reduced, compared to that following treatment with enzalutamide alone or ASCT2 siRNA transfection alone (p < 0.01, 0.01, respectively). After ASCT2 inhibition by siRNA transfection, the growth of 22Rv1 cells was significantly suppressed as compared with negative control siRNA via downregulation of ARV7 both in fetal bovine serum and androgen-deprivation conditions (p < 0.01, 0.01, respectively). We demonstrated that ASCT2 inhibition significantly reduced the proliferation rates of both CSPC and CRPC cells in vitro.
Interval Training in Sports Medicine: Current Thoughts on an Old Idea
Authors: Ketelhut Sascha , Ketelhut Reinhard G. , Weisser Burkhard , Nigg Claudio R.
Published: September 2022

In light of the global physical inactivity pandemic, the increasing prevalence of non-committable diseases, and mounting healthcare costs, effective and feasible prevention and treatment approaches are urgently needed [...]
New Concepts in Diagnosis, Risk Factors and Work Ability in Patients with Hematogenous Spinal Infection
Authors: Korovessis Panagiotis
Published: September 2022

In the last decades, there is an increasing incidence of hematogenous septic spinal infection (HIS) [...]
Head and Neck Cutaneous Soft-Tissue Sarcoma Demonstrate Sex and Racial/Ethnic Disparities in Incidence and Socioeconomic Disparities in Survival
Authors: Jawad Muhammad Umar , Zeitlinger Lauren N. , Bewley Arnaud F. , O’Donnell Edmond F. , Traven Sophia A. , Carr-Ascher Janai R. , Monjazeb Arta M. , Canter Robert J. , Thorpe Steven W. , Randall R. Lor
Published: September 2022

Background: Cutaneous soft-tissue sarcoma (CSTS) of the head and neck are rare and are known to have aggressive clinical course. The current study utilizes a population-based registry in the U.S. to characterize these malignancies and explore disparities. Methods: National Cancer Institute’s (NCI) Surveillance, Epidemiology and End Result (SEER) database from 2000 to 2018 was queried to report incidence and survival data in 4253 cases in the U.S. Results: Males were 5.37 times more likely and Non-Hispanic-White people (NHW) were 4.62 times more likely than females and Non-Hispanic-Black people (NHB) to develop CSTS of the head and neck. The overall incidence was 0.27 per 100,000 persons in 2018, with a significant increase since 2000. Advanced age and stage, histologic group other than ‘fibromatous sarcoma’ and lower SES groups were independent factors for worse overall survival. Conclusions: CSTS of the head and neck demonstrate sex and racial/ethnic disparities in incidence and socioeconomic disparities in overall survival. Level of evidence: II.
Effects of Compression of the Ulnar Artery on the Radial Artery Catheterization
Authors: Kim Cho-Long , Hong Seung-Wan , Kim Seong-Hyop
Published: September 2022

Background: The study was designed to evaluate the effects of compression of the ulnar artery on blood flow (BF) and internal cross-sectional area (CSAi) of the radial artery. We also evaluated the success rate and time of successful ultrasound-guided radial artery catheterization at the first attempt with or without compression of the ulnar artery. Methods: Patients were randomly allocated to the Compression group or Standard group to be treated with or without the application of ulnar artery compression, respectively. Hemodynamic stability was confirmed, and ultrasound-guided radial artery catheterization was performed. In the Compression group, an assistant compressed the ulnar artery at 5 cm above the wrist crease and the catheterization was performed after the loss of the distal ulnar artery BF. In the Standard group, the catheterization was performed without compression of the ulnar artery. Before and after the catheterization, BF and CSAi of the radial artery were evaluated. Success rate and time to successful catheterization at the first attempt were recorded. Results: BF and CSAi of the radial artery were similar in the two groups (37.5 [19.3–66] vs. 37.0 [20.6–53.7] mL/min, respectively, p = 0.63; 4.0 [4.0–6.0] vs. 4.0 [3.0–5.0] mm2, respectively, p = 0.095). In the Compression group, BF and CSAi were changed to 80.9 [35.9–128.5] mL/min (p < 0.001) and 5.0 [4.0–7.0] mm2 (p < 0.001), respectively, after compression of the ulnar artery. There was a trend that the success rate of ultrasound-guided radial artery catheterization at the first attempt was higher in the Compression group than in the Standard group (58/59 vs. 53/59, respectively, p = 0.05), although the difference was not statistically significant. However, the time to successful ultrasound-guided radial artery catheterization at the first attempt was significantly shorter in the Compression group than in the Standard group (34 [27–41] s vs. 46 [36–60] s, p < 0.001). Conclusion: Compression of the ulnar artery augmented BF and CSAi of the radial artery. It resulted in a significantly shorter success time for ultrasound-guided radial artery catheterization at the first attempt.
Energy Homeostasis Gene Nucleotide Variants and Survival of Hemodialysis Patients—A Genetic Cohort Study
Authors: Świderska Monika , Mostowska Adrianna , Skrypnik Damian , Jagodziński Paweł , Bogdański Paweł , Grzegorzewska Alicja
Published: September 2022

Background: Patients undergoing hemodialysis (HD) therapy have an increased risk of death compared to the general population. We investigated whether selected single nucleotide variants (SNVs) involved in glucose and lipid metabolism are associated with mortality risk in HD patients. Methods: The study included 805 HD patients tested for 11 SNVs in FOXO3, IGFBP3, FABP1, PCSK9, ANGPTL6, and DOCK6 using HRM analysis and TaqMan assays. FOXO3, IGFBP3, L-FABP, PCSK9, ANGPTL6, and ANGPTL8 plasma concentrations were measured by ELISA in 86 individuals. The Kaplan–Meier method and Cox proportional hazards models were used for survival analyses. Results: We found out that the carriers of a C allele in ANGPTL6 rs8112063 had an increased risk of all-cause, cardiovascular, and cardiac mortality. In addition, the C allele of DOCK6 rs737337 was associated with all-cause and cardiac mortality. The G allele of DOCK6 rs17699089 was correlated with the mortality risk of patients initiating HD therapy. The T allele of FOXO3 rs4946936 was negatively associated with cardiac and cardiovascular mortality in HD patients. We observed no association between the tested proteins’ circulating levels and the survival of HD patients. Conclusions: The ANGPTL6 rs8112063, FOXO3 rs4946936, DOCK6 rs737337, and rs17699089 nucleotide variants are predictors of survival in patients undergoing HD.
MicroShunt versus Trabeculectomy for Surgical Management of Glaucoma: A Retrospective Analysis
Authors: Fu Michael X. , Normando Eduardo M. , Luk Sheila M. H. , Deshmukh Mira , Ahmed Faisal , Crawley Laura , Ameen Sally , Vig Niten , Cordeiro Maria Francesca , Bloom Philip A.
Published: September 2022

This case-control study aims to compare the efficacy, safety, and postoperative burden of MicroShunt versus trabeculectomy. The first consecutive cohort of MicroShunt procedures (n = 101) was matched to recent historical trabeculectomy procedures (n = 101) at two London hospital trusts. Primary endpoints included changes in intraocular pressure (IOP) and glaucoma medications. Secondary outcome measures included changes in retinal nerve fibre layer (RNFL) thickness, rates of complications, further theatre interventions, and the number of postoperative visits. From the baseline to Month-18, the median [interquartile range] IOP decreased from 22 [17–29] mmHg (on 4 [3–4] medications) to 15 [10–17] mmHg (on 0 [0–2] medications) and from 20 [16–28] mmHg (on 4 [3–4] medications) to 11 [10–13] mmHg (on 0 [0–0] medications) in the MicroShunt and trabeculectomy groups, respectively. IOP from Month-3 was significantly higher in the MicroShunt group (p = 0.006), with an increased number of medications from Month-12 (p = 0.024). There were greater RNFL thicknesses from Month-6 in the MicroShunt group (p = 0.005). The rates of complications were similar (p = 0.060) but with fewer interventions (p = 0.031) and postoperative visits (p = 0.001) in the MicroShunt group. Therefore, MicroShunt has inferior efficacy to trabeculectomy in lowering IOP and medications but provides a better safety profile and postoperative burden and may delay RNFL loss.
Vitamin D Status and Steatohepatitis in Obese Diabetic and Non-Diabetic Patients
Authors: Taban Laura , Stoian Dana , Timar Bogdan , Amzar Daniela , Adela Calin , Motofelea Alexandru , Borlea Andreea , Frisoni Romain , Laguerre Nadege
Published: September 2022

Background and Aims: The presence of steatohepatitis in obese patients can be multifactorial. The current study tries to determine the differences between diabetic and non-diabetic patients regarding the presence of steatohepatitis. We evaluated sequential liver samples and collected the times of bariatric surgery to assess the presence of NASH in patients with obesity, in the circuit of bariatric surgery. Methods: We performed a retrospective study of 49 patients presenting high-grade obesity in the circuit of bariatric surgery, with liver biopsy. The patients underwent bariatric surgery at a single center in France and were followed for 2 years. The liver biopsies were performed intraoperatively on all 49 patients before the bariatric surgery. The primary endpoint of the study was to evaluate the relationships between steatohepatitis/liver fibrosis and the presence of diabetes and to evaluate the current relationships between the biochemical work-ups. Special importance was accorded to the correlations between vitamin D levels and the presence of hepatic steatosis, due to the antifibrogenic pattern in the liver, as shown in many important papers in the field. Results: Significant correlations were found between the presence of liver fibrosis and the presence of diabetes (p = 0.022), but not regarding the antidiabetic treatment. An important correlation was found between the vitamin D levels and the presence of liver fibrosis, as well as with the levels of A1C hemoglobin and LDL cholesterol levels. Conclusions: Vitamin D deficiency presents a strong correlation with hepatic steatosis in individuals with morbid obesity. Correcting vitamin D deficiency may present a beneficial role in treating hepatic steatosis, diabetes, and cardiovascular risk in patients with morbid obesity.
Buckling Analysis of Functionally Graded Materials (FGM) Thin Plates with Various Circular Cutout Arrangements
Authors: Alashkar Adnan , Elkafrawy Mohamed , Hawileh Rami , AlHamaydeh Mohammad
Published: September 2022

In this paper, several analyses were conducted to investigate the buckling behavior of Functionally Graded Material (FGM) thin plates with various circular cutout arrangements. The computer model was simulated using the Finite Element (FE) software ABAQUS. The developed model was validated by the authors in previous research. A parametric analysis was employed to investigate the effect of plate thickness and circular cutout diameter on the buckling behavior of the FGM thin plates. The normalized buckling load was also calculated to compare the buckling performance of FGM plates with various dimensions. Moreover, von Mises stress analysis was examined to understand the yield capability of the FGM plates in addition to the buckling modes that show the stress distribution of the critical buckling stress. Hence, this research provides a comprehensive analysis to display the relation between the critical buckling load and the arrangement of the circular cutouts. The results show that the critical buckling load heavily depends on the dimension of the plate and the cutout size. For instance, an increase in the plate thickness and a decrease in the cutout diameter increase the critical buckling load. Moreover, the circular cutout in a horizontal arrangement exhibited the best buckling performance, and as the arrangement shifts to a vertical arrangement, the buckling performance deteriorates.
New Equations for Hydrostatic Weighing without Head Submersion
Authors: Tesch Jeff C. , Papadopoulos Panayiotis , Dolgener Forrest , Tinsley Grant M.
Published: September 2022

New equations were derived to predict the density of the body (DB) by hydrostatic weighing with the head above water (HWHAW). Hydrostatic weighing with the head below water (HWHBW) was the criterion for DB measurement in 90 subjects (44 M, 46 F). Head volume by immersion (HVIMM) was determined by subtracting the mass in water with the head below water (MWHBW) from the mass in water with the head above water (MWHAW), with subjects at residual lung volume. Equations were derived for head volume prediction (HVPRED) from head measurements and used to correct DB by HWHAW. Equations were also derived for HWHAW using direct regression of DB from uncorrected density (with MWHAW in place of MWHBW). Prediction equations were validated in 45 additional subjects (21 M, 24 F). Results were evaluated using equivalence testing, linear regression, Bland–Altman plots, and paired t-tests. Head girth, face girth, and body mass produced the smallest errors for HVPRED. In both M and F validation groups, equivalence (±2% fat by weight) was demonstrated between body fat percent (BF%) by HWHBW and BF% by HWHAW with HVPRED. Variance in computer-averaged samples of MWHAW was significantly less (p < 0.05) than MWHBW. Prediction error was smaller for BF% by HWHAW with HVPRED than for alternative methods. Conclusions: Equivalence between BF% by HWHBW and BF% by HWHAW with HVPRED was demonstrated and differences were not statistically significant. Weight fluctuations were smaller for HWHAW than HWHBW.
Downsizing the Linear PM Generator in Wave Energy Conversion for Improved Economic Feasibility
Authors: Tan Jian , Wang Xuezhou , Polinder Henk , Laguna Antonio Jarquin , Miedema Sape A.
Published: September 2022

A crucial part of wave energy converters (WECs) is the power take-off (PTO) mechanism, and PTO sizing has been shown to have a considerable impact on the levelized cost of energy (LCOE). However, as a dominating type of PTO system in WECs, previous research pertinent to PTO sizing did not take modeling and optimization of the linear permanent magnet (PM) generator into consideration. To fill this gap, this paper provides an insight into how PTO sizing affects the performance of linear permanent magnet (PM) generators, and further the techno-economic performance of WECs. To thoroughly reveal the power production of the WEC, both hydrodynamic modeling and generator modeling are incorporated. In addition, three different methods for sizing the linear generator are applied and compared. The effect of the selection of the sizing method on the techno-economic performance of the WEC is identified. Furthermore, to realistically reflect the relevance of PTO sizing, wave resources from three European sea sites are considered in the techno-economic analysis. The dependence of PTO sizing on wave resources is demonstrated.
Dependence of Convective Cloud Properties and Their Transport on Cloud Fraction and GCM Resolution Diagnosed from a Cloud-Resolving Model Simulation
Authors: Zhang Zhanjie , Zhang Guang J.
Published: September 2022

The scale-aware convective parameterization for high resolution global climate models must satisfy the requirement that the parameterized subgrid convective transport diminishes as the model resolution increases to convection-resolving resolutions. A major assumption in current scale-aware convection schemes is that the differences between convective cloud properties and their environmental counterparts are independent of cloud fraction. This study examines convective cloud vertical velocity, moist static energy (MSE), moisture, and the vertical eddy transport of MSE and moisture for different averaging subdomain sizes and fractional convective cloudiness using a cloud resolving model simulation of a midlatitude mesoscale convective system. Results show that convective cloud fraction, mass flux, and vertical transport of MSE and moisture increase with decreasing subdomain size. The differences between convective cloud properties in both updrafts and downdrafts and their environment depend on both cloud fraction and the averaging subdomain size. For a given subdomain size, the differences increase with cloud fraction, in contrast to the assumption used in current scale-aware convection parameterization schemes. A consequence of this is that the parameterized convective eddy transport reaches maximum at a higher cloud fraction than believed in previous studies. This has implications on how fast the subgrid convective transport should diminish as GCM resolution increases.
Incorporation of Deep Kernel Convolution into Density Clustering for Shipping AIS Data Denoising and Reconstruction
Authors: Zhang Jufu , Ren Xujie , Li Huanhuan , Yang Zaili
Published: September 2022

Automatic Identification System (AIS) equipment can aid in identifying ships, reducing ship collision risks and ensuring maritime safety. However, the explosion of massive AIS data has caused increasing data processing challenges affecting their practical applications. Specifically, mistakes, noise, and missing data are presented during AIS data transmission and encoding, resulting in poor data quality and inaccurate data sources that negatively impact maritime safety research. To address this issue, a robust AIS data denoising and reconstruction methodology was proposed to realise the data preprocessing for different applications in maritime transportation. It includes two parts: Density-Based Spatial Clustering of Applications with Noise based on Deep Kernel Convolution (DBSCANDKC) and the reconstruction method, which can extract high-quality AIS data to guarantee the accuracy of the related maritime research. Firstly, the kinematics feature was employed to remove apparent noise from the AIS data. The square deep kernel convolution was then incorporated into density clustering to find and remove possibly anomalous data. Finally, a piecewise cubic spline interpolation approach was applied to construct the missing denoised trajectory data. The experiments were implemented in the Arctic Ocean and Strait of Dover to demonstrate the effectiveness and performance of the proposed methodology in different shipping environments. This methodology makes significant contributions to future maritime situational awareness, collision avoidance, and robust trajectory development for safety at sea.
Hydraulic Performance of Geotextile Sand Containers for Coastal Defenses
Authors: Corvaro Sara , Lorenzoni Carlo , Mancinelli Alessandro , Marini Francesco , Rocchi Stefania
Published: September 2022

Laboratory experiments were performed in the wave flume of the Laboratorio di Idraulica e Costruzioni Marittime of the Università Politecnica delle Marche (Ancona, Italy) to study the hydrodynamic performance of coastal protection structures made of a new type of geotextile sand containers (GSCs). Such structures are used as softer and flexible alternatives to traditional hard coastal defenses made of concrete or rubble mound material. The GSC structures can also be used as temporary coastal protections during the winter period. The physical model reproduced two main configurations: in the former one, the GSCs were used as coastal revetments with three different slopes. In the latter one, the GSCs were applied to make detached submerged breakwaters with different submergences and berm widths. The geometric scale of the models was 1:10, and the weight of each GSC in the prototype was 5 t. The geotextile material of the containers and the wave characteristics were reproduced by using the Reynolds and the Froude similarity criteria, respectively. Reflection coefficients and hydraulic stability behaviors for the revetments, as well as transmission coefficients and piling-up amount for breakwaters, were obtained.
Organic Geochemical Signatures of the Upper Miocene (Tortonian—Messinian) Sedimentary Succession Onshore Crete Island, Greece: Implications for Hydrocarbon Prospectivity
Authors: Maravelis Angelos G. , Kontakiotis George , Bellas Spyridon , Antonarakou Assimina , Botziolis Chrysanthos , Janjuhah Hammad Tariq , Makri Panayota , Moissette Pierre , Cornée Jean-Jacques , Pasadakis Nikolaos , Manoutsoglou Emmanouil , Zelilidis Avraam , Karakitsios Vasileios
Published: September 2022

The definition of pre-Messinian source rocks in the eastern Mediterranean is of paramount importance for hydrocarbon exploration because of the ability of salt to act as a high-quality seal rock. This research evaluates the organic geochemical features of the Upper Miocene (Tortonian—Messinian) sedimentary succession onshore Crete Island, Greece. The study employs original (Messinian, Agios Myron Fm) and published (Tortonian, Viannos Fm, Skinias Fm, Moulia Fm, and Messinian Ploutis section) results from organic geochemical analyses of mudstone samples. One hundred and one samples were examined using standard organic geochemistry methodology (Rock-Eval II and VI-TOC) to define the origin, type, and degree of organic matter maturity. The data indicate that the studied samples have poor to fair gas-prone source rock potential. These possible source rock units have not experienced great temperatures during burial, and, thus, their organic matter is thermally immature. The sub-salt (Tortonian—Messinian) source rock units are likely to be of higher thermal maturity in the western and eastern south Cretan trenches because of tectonic subsidence and a thicker sedimentary overburden. Several traps can grow in these regions, associated with normal faults, rotated blocks and unconformities (both below and above the unconformities). This research provides a basis for the further evaluation of the hydrocarbon potential in Crete Island. It is an area that shares geological similarities with the surrounding regions that contain proven reserves and is of crucial economic and strategic importance.
Insights on KP4 Killer Toxin-like Proteins of Fusarium Species in Interspecific Interactions
Authors: Vicente Isabel , Quaratiello Giuseppe , Baroncelli Riccardo , Vannacci Giovanni , Sarrocco Sabrina
Published: September 2022

KP4 killer toxins are secreted proteins that inhibit cell growth and induce cell death in target organisms. In Fusarium graminearum, KP4-like (KP4L) proteins contribute to fungal virulence in wheat seedling rot and are expressed during Fusarium head blight development. However, fungal KP4L proteins are also hypothesized to support fungal antagonism by permeabilizing cell walls of competing fungi to enable penetration of toxic compounds. Here, we report the differential expression patterns of F. graminearum KP4L genes (Fgkp4l-1, -2, -3 and -4) in a competitive interaction, using Trichoderma gamsii as the antagonist. The results from dual cultures indicate that Fgkp4l-3 and Fgkp4l-4 could participate in the recognition at the distance of the antagonist, while all Fgkp4l genes were highly activated in the pathogen during the physical interaction of both fungi. Only Fgkp4l-4 was up-regulated during the interaction with T. gamsii in wheat spikes. This suggests the KP4L proteins could participate in supporting F. graminearum interspecific interactions, even in living plant tissues. The distribution of KP4L orthologous within the genus Fusarium revealed they are more represented in species with broad host-plant range than in host-specific species. Phylogeny inferred provides evidence that KP4L genes evolved through gene duplications, gene loss and sequence diversification in the genus Fusarium.
Unveiling the Secretome of the Fungal Plant Pathogen Neofusicoccum parvum Induced by In Vitro Host Mimicry
Authors: Nazar Pour Forough , Pedrosa Bruna , Oliveira Micaela , Fidalgo Cátia , Devreese Bart , Driessche Gonzalez Van , Félix Carina , Rosa Nuno , Alves Artur , Duarte Ana Sofia , Esteves Ana Cristina
Published: September 2022

Neofusicoccum parvum is a fungal plant pathogen of a wide range of hosts but knowledge about the virulence factors of N. parvum and host–pathogen interactions is rather limited. The molecules involved in the interaction between N. parvum and Eucalyptus are mostly unknown, so we used a multi-omics approach to understand pathogen–host interactions. We present the first comprehensive characterization of the in vitro secretome of N. parvum and a prediction of protein–protein interactions using a dry-lab non-targeted interactomics strategy. We used LC-MS to identify N. parvum protein profiles, resulting in the identification of over 400 proteins, from which 117 had a different abundance in the presence of the Eucalyptus stem. Most of the more abundant proteins under host mimicry are involved in plant cell wall degradation (targeting pectin and hemicellulose) consistent with pathogen growth on a plant host. Other proteins identified are involved in adhesion to host tissues, penetration, pathogenesis, or reactive oxygen species generation, involving ribonuclease/ribotoxin domains, putative ricin B lectins, and necrosis elicitors. The overexpression of chitosan synthesis proteins during interaction with the Eucalyptus stem reinforces the hypothesis of an infection strategy involving pathogen masking to avoid host defenses. Neofusicoccum parvum has the molecular apparatus to colonize the host but also actively feed on its living cells and induce necrosis suggesting that this species has a hemibiotrophic lifestyle.
Performance of Multiplex PCR and β-1,3-D-Glucan Testing for the Diagnosis of Candidemia
Authors: Koc Özlem , Kessler Harald H. , Hoenigl Martin , Wagener Johannes , Suerbaum Sebastian , Schubert Sören , Dichtl Karl
Published: September 2022

Bloodstream infections caused by Candida yeasts (candidemia) are associated with high morbidity and mortality. Diagnosis remains challenging, with the current gold standard—isolation from blood culture (BC)—being limited by low sensitivity and long turnaround time. This study evaluated the performance of two nonculture methods: PCR and β-1,3-D-glucan (BDG) testing. The sera of 103 patients with BC-proven candidemia and of 46 controls were analyzed with the Fungiplex Candida Real-Time PCR and the Wako β-Glucan Test. The BDG assay demonstrated higher sensitivity than the multiplex PCR (58% vs. 33%). This was particularly evident in ICU patients (60% vs. 28%) and in C. albicans candidemia (57% vs. 37%). The earlier prior to BC sampling the sera were obtained, the more the PCR sensitivity decreased (46% to 18% in the periods of 0–2 and 3–5 days before BC, respectively), while BDG testing was independent of the sampling date. No positive PCR results were obtained in sera sampled more than five days before BC. Specificities were 89% for BDG and 93% for PCR testing. In conclusion, BDG testing demonstrated several advantages over PCR testing for the diagnosis of candidemia, including higher sensitivity and earlier diagnosis. However, BC remains essential, as BDG does not allow for species differentiation.
Phytophthora × cambivora as a Major Factor Inciting the Decline of European Beech in a Stand within the Southernmost Limit of Its Natural Range in Europe
Authors: Riolo Mario , Aloi Francesco , Conti Taguali Sebastiano , Pane Antonella , Franco Massimo , Cacciola Santa Olga
Published: September 2022

The objective of this study was to investigate the role of the oomycete Phytophthora× cambivora in the decline affecting European beech (Fagus sylvatica) in the Nebrodi Regional Park (Sicily, southern Italy). In a survey of a beech forest stand in the heart of the park, Phytophthora× cambivora was the sole Phytophthora species recovered from the rhizosphere soil and fine roots of trees. Both A1 and A2 mating type isolates were found. Direct isolation from the stem bark of trees showing severe decline symptoms and bleeding stem cankers yielded exclusively P. gonapodyides, usually considered as an opportunistic pathogen. The mean inoculum density of P.× cambivora in the rhizosphere soil, as determined using the soil dilution plating method and expressed in terms of colony forming units (cfus) per gm of soil, the isolation frequency using leaf baiting, and the percentage of infected fibrous roots from 20 randomly selected beech trees with severe decline symptoms (50 to 100 foliage transparency classes) were 31.7 cfus, 80%, and 48.6%, respectively. These were significantly higher than the corresponding mean values of 20 asymptomatic or slightly declining trees, suggesting P.× cambivora is a major factor responsible for the decline in the surveyed stand.