bluuebunny/crossref_metadata_2025 huggingface dataset:
Photostabilization of Poly(vinyl chloride) Films Blended with Organotin Complexes of Mefenamic Acid for Outdoor Applications
Authors: Ahmed Ahmed , El-Hiti Gamal A. , Hadi Angham G. , Ahmed Dina S. , Baashen Mohammed A. , Hashim Hassan , Yousif Emad
Published: March 2021

This study develops a process for enhancing the photostabilization of PVC films blended with a low concentration of mefenamate–tin complex. One tri-substituted and three di-substituted organotin complexes containing mefenamate unit are synthesized, and their chemical structures are established. The reactions of mefenamic acid and a number of substituted tin chlorides gave the corresponding tin complexes in 70–77% yields. Tin complexes were blended with PVC and thin films. The effect of the addition of additives against long-term irradiation (290–400 nm, 300 h) is also tested. Changes in the infrared spectra, weight, and surface of the PVC blends due to irradiation are examined and analyzed. Any damage to the PVC surface and its chemical degradation level are noticeably low in the presence of additives. Minimal photodegradation levels and surface changes of the irradiated PVC films are observed when the triphenyltin complex is used. Such a complex is highly aromatic and can act as an ultraviolet irradiation absorber and a scavenger for hydrogen chloride and radicals produced due to the photooxidation and photoirradiation of PVC films.
Learning a Swarm Foraging Behavior with Microscopic Fuzzy Controllers Using Deep Reinforcement Learning
Authors: Aznar Fidel , Pujol Mar , Rizo Ramón
Published: March 2021

This article presents a macroscopic swarm foraging behavior obtained using deep reinforcement learning. The selected behavior is a complex task in which a group of simple agents must be directed towards an object to move it to a target position without the use of special gripping mechanisms, using only their own bodies. Our system has been designed to use and combine basic fuzzy behaviors to control obstacle avoidance and the low-level rendezvous processes needed for the foraging task. We use a realistically modeled swarm based on differential robots equipped with light detection and ranging (LiDAR) sensors. It is important to highlight that the obtained macroscopic behavior, in contrast to that of end-to-end systems, combines existing microscopic tasks, which allows us to apply these learning techniques even with the dimensionality and complexity of the problem in a realistic robotic swarm system. The presented behavior is capable of correctly developing the macroscopic foraging task in a robust and scalable way, even in situations that have not been seen in the training phase. An exhaustive analysis of the obtained behavior is carried out, where both the movement of the swarm while performing the task and the swarm scalability are analyzed.
Foot Contact Dynamics and Fall Risk among Children Diagnosed with Idiopathic Toe Walking
Authors: Soangra Rahul , Shiraishi Michael , Beuttler Richard , Gwerder Michelle , Boyd LouAnne , Muthukumar Venkatesan , Trabia Mohamed , Aminian Afshin , Grant-Beuttler Marybeth
Published: March 2021

Children that are diagnosed with Idiopathic Toe walking (cITW) are characterized by persistent toe-to-toe contacts. The objective of this study was to explore whether typical foot contact dynamics during walking predisposes cITW to a higher risk of falling. Twenty cITW and age-matched controls performed typical and toe walking trials. The gait parameters related to foot contact dynamics, vertical force impulses during stance, slip, and trip risk were compared for both groups. We found that cITW manifest less stable gait and produced significantly higher force impulses during push-off. Additionally, we found that cITW had a higher slip-initiation risk that was associated with higher foot contact horizontal and vertical velocities in addition to lower transitional acceleration of center of mass. We found that cITW exhibited a higher trip risk with toe clearance being significantly lower when compared to healthy counterparts. This study allowed for a quantitative description of foot contact dynamics and delineated typical from toe walking among cITW. Overall, the results indicate that cITW are less stable during typical walking and are prone to a higher risk of slip and trip-like falls.
Abrasive Surface Finishing on SLM 316L Parts Fabricated with Recycled Powder
Authors: Mesicek Jakub , Ma Quoc-Phu , Hajnys Jiri , Zelinka Jan , Pagac Marek , Petru Jana , Mizera Ondrej
Published: March 2021

Improving the surface roughness quality of 3D printed components, especially metallic ones, which are fabricated from the selective laser melting (SLM) method, has drawn enormous attention from the research community. It should be noted that various studies on this topic have reported that precise surface roughness results can be obtained with various techniques that are indeed not cost-effective. Differing itself from these studies, this manuscript investigates an economical solution for fabricating and surface treating SLM components. Specifically, the inspected specimens were printed with recycled 316L stainless steel powder and treated solely with two abrasive surface finishing methods. In the manuscript, two scanning strategies namely meander and stripes, and three types of surfaces were investigated. Subsequently, their 2D and 3D surface roughness results were elaborated. After the proposed herein abrasive treatment, 3D surface roughness arithmetical mean height of a surface (Sa) value of 0.9 µm can be achieved.
The Application of Virtual Reality in Engineering Education
Authors: Soliman Maged , Pesyridis Apostolos , Dalaymani-Zad Damon , Gronfula Mohammed , Kourmpetis Miltiadis
Published: March 2021

The advancement of VR technology through the increase in its processing power and decrease in its cost and form factor induced the research and market interest away from the gaming industry and towards education and training. In this paper, we argue and present evidence from vast research that VR is an excellent tool in engineering education. Through our review, we deduced that VR has positive cognitive and pedagogical benefits in engineering education, which ultimately improves the students’ understanding of the subjects, performance and grades, and education experience. In addition, the benefits extend to the university/institution in terms of reduced liability, infrastructure, and cost through the use of VR as a replacement to physical laboratories. There are added benefits of equal educational experience for the students with special needs as well as distance learning students who have no access to physical labs. Furthermore, recent reviews identified that VR applications for education currently lack learning theories and objectives integration in their design. Hence, we have selected the constructivist and variation learning theories as they are currently successfully implemented in engineering education, and strong evidence shows suitability of implementation in VR for education.
Multi-Layer Reflectivity Calculation Based Meta-Modeling of the Phase Mapping Function for Highly Reproducible Surface Plasmon Resonance Biosensing
Authors: Wu Tzu-Heng , Yang Ching-Hsu , Chang Chia-Chen , Liu Hui-Wen , Yang Chia-Yu , Shen Tang-Long , Lin Chii-Wann , Bruyant Aurélien
Published: March 2021

Phase-sensitive surface plasmon resonance biosensors are known for their high sensitivity. One of the technology bottle-necks of such sensors is that the phase sensorgram, when measured at fixed angle set-up, can lead to low reproducibility as the signal conveys multiple data. Leveraging the sensitivity, while securing satisfying reproducibility, is therefore is an underdiscussed key issue. One potential solution is to map the phase sensorgram into refractive index unit by the use of sensor calibration data, via a simple non-linear fit. However, basic fitting functions poorly portray the asymmetric phase curve. On the other hand, multi-layer reflectivity calculation based on the Fresnel coefficient can be employed for a precise mapping function. This numerical approach however lacks the explicit mathematical formulation to be used in an optimization process. To this end, we aim to provide a first methodology for the issue, where mapping functions are constructed from Bayesian optimized multi-layer model of the experimental data. The challenge of using multi-layer model as optimization trial function is addressed by meta-modeling via segmented polynomial approximation. A visualization approach is proposed for assessment of the goodness-of-the-fit on the optimized model. Using metastatic cancer exosome sensing, we demonstrate how the present work paves the way toward better plasmonic sensors.
Paediatricians’ Views on Pain in Children with Profound Intellectual and Multiple Disabilities
Authors: Petigas Lucie , Newman Christopher J.
Published: March 2021

Pain is a frequent issue in children with profound intellectual and multiple disabilities (PIMD). Its identification and treatment can prove highly challenging for primary care physicians, mainly because of the children’s limited communication abilities. We used an online survey to explore paediatricians’ views regarding the experience and management of pain in children with PIMD and invited 480 professionals working in the canton of Vaud, Switzerland, to take part. We received 121 responses (participation rate 25.5%). A large majority of respondents provided care to children with PIMD. All paediatricians considered that these children feel pain at least as much as typically developing children. However, paediatricians had mixed views on their tolerance to pain. More than 90% held the view that their pain is under-assessed and undertreated. The principal barriers they reported to appropriate management were communication limitations with the child, difficulties in pain assessment, lack of knowledge about children with disabilities and lack of experience. Paediatricians have complex opinions regarding how children with PIMD experience pain and how to manage this problem. Professional education and training on the specificities of children with PIMD, including how to address their pain, seem necessary to foster paediatricians’ ability and confidence in approaching this complex issue.
Unintended Regulatory Caused Early Death—A Difficult Endpoint in Cancer Patient Care and Treatment
Authors: Berdel Wolfgang E.
Published: March 2021

The pharmacological armory against cancer has been growing, with many new drugs approved. The Good Clinical Practice (GCP)-based Clinical Trials Directive was adopted in the EU in 2001, with the important objectives of achieving better patient safety and improved quality of clinical trial conduct. However, clinical experience with the implementation of the regulation raises the question as to whether aspects of this regulatory framework can cause harm to some patients. This question also arises in daily clinical cancer patient care when the time between the publication of pivotal study results and their approval, and details of post-approval regulations, are scrutinized. Clinical observations, provocatively summarized as “unintended regulatory caused early death”, are discussed.
Deletion of the Actin-Associated Tropomyosin Tpm3 Leads to Reduced Cell Complexity in Cultured Hippocampal Neurons—New Insights into the Role of the C-Terminal Region of Tpm3.1
Authors: Tomanić Tamara , Martin Claire , Stefen Holly , Parić Esmeralda , Gunning Peter , Fath Thomas
Published: March 2021

Tropomyosins (Tpms) have been described as master regulators of actin, with Tpm3 products shown to be involved in early developmental processes, and the Tpm3 isoform Tpm3.1 controlling changes in the size of neuronal growth cones and neurite growth. Here, we used primary mouse hippocampal neurons of C57/Bl6 wild type and Bl6Tpm3flox transgenic mice to carry out morphometric analyses in response to the absence of Tpm3 products, as well as to investigate the effect of C-terminal truncation on the ability of Tpm3.1 to modulate neuronal morphogenesis. We found that the knock-out of Tpm3 leads to decreased neurite length and complexity, and that the deletion of two amino acid residues at the C-terminus of Tpm3.1 leads to more detrimental changes in neurite morphology than the deletion of six amino acid residues. We also found that Tpm3.1 that lacks the 6 C-terminal amino acid residues does not associate with stress fibres, does not segregate to the tips of neurites, and does not impact the amount of the filamentous actin pool at the axonal growth cones, as opposed to Tpm3.1, which lacks the two C-terminal amino acid residues. Our study provides further insight into the role of both Tpm3 products and the C-terminus of Tpm3.1, and it forms the basis for future studies that aim to identify the molecular mechanisms underlying Tpm3.1 targeting to different subcellular compartments.
Elementary Students’ Knowledge Development during the Implementation of “After School Exercise” Program
Authors: Syrmpas Ioannis , Goudas Marios
Published: March 2021

Physical education should focus not only on students’ motor and emotional development but also on their cognitive development. The purpose of the present study was to examine whether elementary students’ health-related knowledge and physical activity-related goal setting increased after they participated in a program. The program aimed at promoting after school physical activity among students. Participants were 244 fifth- and sixth-grade students (116 boys and 128 girls). Students’ knowledge was examined by means of a multiple-choice test, cognitive assignments and a retrospective pre–post questionnaire. The results from the three measures indicated that students enhanced their knowledge both regarding the health benefits of physical activity (PA) and effective goal setting. Hence, it can be argued that the program was effective in promoting students’ skills and knowledge related to PA.
Electronic Health Records in Specialized Pediatric Palliative Care: A Qualitative Needs Assessment among Professionals Experienced and Inexperienced in Electronic Documentation
Authors: Meyer Dorothee , Kernebeck Sven , Busse Theresa Sophie , Ehlers Jan , Wager Julia , Zernikow Boris , Dreier Larissa Alice
Published: March 2021

Background: Currently, to the best of our knowledge, no findings exist concerning the needs of professionals in specialized pediatric palliative care (PPC) regarding electronic health records (EHRs). Several studies have highlighted benefits concerning the use of EHRs in pediatrics. However, usability is strongly affected by the degree of adaptivity to the context of application. The aim of this study is to examine the needs of professionals concerning an EHR in the specialized PPC inpatient and outpatient settings. Methods: A qualitative research design was chosen to address the complex aspects of user demands. Focus group interviews and semi-structured one-on-one interviews were conducted with PPC professionals. N = 23 participants from inpatient and N = 11 participants from outpatient settings of specialized PPC representing various professions took part in the study. Results: The findings could be grouped into four categories: (1) attitude towards the current methods of documentation, (2) attitude towards electronic documentation in general, (3) general requirements for an EHR, and (4) content requirements for an EHR. Conclusions: Professionals in specialized PPC expect and experience many benefits of using electronic documentation. Their requirements for an EHR for inpatient and outpatient settings of PPC are largely consistent with EHRs for pediatrics. However, individual specifications and adaptations are necessary for this particular setting.
Quality Indicators in Pediatric Palliative Care: Considerations for Latin America
Authors: Zuniga-Villanueva Gregorio , Ramos-Guerrero Jorge Alberto , Osio-Saldaña Monica , Casas Jessica A. , Marston Joan , Okhuysen-Cawley Regina
Published: March 2021

Pediatric palliative care is a growing field in which the currently available resources are still insufficient to meet the palliative care needs of children worldwide. Specifically, in Latin America, pediatric palliative care services have emerged unevenly and are still considered underdeveloped when compared to other regions of the world. A crucial step in developing pediatric palliative care (PPC) programs is delineating quality indicators; however, no consensus has been reached on the outcomes or how to measure the impact of PPC. Additionally, Latin America has unique sociocultural characteristics that impact the perception, acceptance, enrollment and implementation of palliative care services. To date, no defined set of quality indicators has been proposed for the region. This article explores the limitations of current available quality indicators and describes the Latin American context and how it affects PPC development. This information can help guide the creation of standards of care and quality indicators that meet local PPC needs while considering the sociocultural landscape of Latin America and its population.
Microtubules as One-Dimensional Crystals: Is Crystal-Like Structure the Key to the Information Processing of Living Systems?
Authors: Sanchez-Castro Noemí , Palomino-Ovando Martha Alicia , Singh Pushpendra , Sahu Satyajit , Toledo-Solano Miller , Faubert Jocelyn , Lugo J. Eduardo , Bandyopadhyay Anirban , Ray Kanad
Published: March 2021

Each tubulin protein molecule on the cylindrical surface of a microtubule, a fundamental element of the cytoskeleton, acts as a unit cell of a crystal sensor. Electromagnetic sensing enables the 2D surface of microtubule to act as a crystal or a collective electromagnetic signal processing system. We propose a model in which each tubulin dimer acts as the period of a one-dimensional crystal with effective electrical impedance related to its molecular structure. Based on the mathematical crystal theory with one-dimensional translational symmetry, we simulated the electrical transport properties of the signal across the microtubule length and compared it to our single microtubule experimental results. The agreement between theory and experiment suggests that one of the most essential components of any Eukaryotic cell acts as a one-dimensional crystal.
Synthesis and Characterization of Potent and Safe Ciprofloxacin-Loaded Ag/TiO2/CS Nanohybrid against Mastitis Causing E. coli
Authors: Zafar Naheed , Uzair Bushra , Niazi Muhammad Bilal Khan , Samin Ghufrana , Bano Asma , Jamil Nazia , Waqar-Un-Nisa  , Sajjad Shamaila , Menaa Farid
Published: March 2021

To improve the efficacy of existing classes of antibiotics (ciprofloxacin), allow dose reduction, and minimize related toxicity, this study was executed because new target-oriented livestock antimicrobials are greatly needed to battle infections caused by multidrug-resistant (MDR) strains. The present study aims to green synthesize a biocompatible nanohybrid of ciprofloxacin (CIP)-Ag/TiO2/chitosan (CS). Silver and titanium nanoparticles were green synthesized using Moringa concanensis leaves extract. The incorporation of silver (Ag) nanoparticles onto the surface of titanium oxide nanoparticles (TiO2NPs) was done by the wet chemical impregnation method, while the encapsulation of chitosan (CS) around Ag/TiO2 conjugated with ciprofloxacin (CIP) was done by the ionic gelation method. The synthesized nanohybrid (CIP-Ag/TiO2/CS) was characterized using standard techniques. The antibacterial potential, killing kinetics, cytotoxicity, drug release profile, and minimum inhibitory concentration (MIC) were determined. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) revealed spherical agglomerated nanoparticles (NPs) of Ag/TiO2 with particle sizes of 47–75 nm, and those of the CIP-Ag/TiO2/CS nanohybrid were in range of 20–80 nm. X-ray diffractometer (XRD) patterns of the hetero system transmitted diffraction peaks of anatase phase of TiO2 and centered cubic metallic Ag crystals. Fourier Transform Infrared spectroscopy (FTIR) confirmed the Ti-O-Ag linkage in the nanohybrid. The zeta potential of CIP-Ag/TiO2/CS nanohybrid was found (67.45 ± 1.8 mV), suggesting stable nanodispersion. The MIC of CIP-Ag/TiO2/CS was 0.0512 μg/mL, which is much lower than the reference value recorded by the global CLSI system (Clinical Laboratory Standards Institute). The CIP-Ag/TiO2/CS nanohybrid was found to be effective against mastitis causing MDR E. coli; killing kinetics showed an excellent reduction of E. coli cells at 6 h of treatment. Flow cytometry further confirmed antibacterial potential by computing 67.87% late apoptosis feature at 6 h of treatment; antibiotic release kinetic revealed a sustained release of CIP. FESEM and TEM confirmed the structural damages in MDR E. coli (multidrug-resistant Escherichia coli). The CIP-Ag/TiO2/CS nanohybrid was found to be biocompatible, as more than 93.08% of bovine mammary gland epithelial cells remained viable. The results provide the biological backing for the development of nanohybrid antibiotics at a lower MIC value to treat infectious diseases of cattle and improve the efficacy of existing classes of antibiotics by conjugation with nanoparticles.
Minimally Invasive Intracerebral Hemorrhage Evacuation Techniques: A Review
Authors: Hannah Theodore C. , Kellner Rebecca , Kellner Christopher P.
Published: March 2021

Intracerebral hemorrhage (ICH) continues to have high morbidity and mortality. Improving ICH outcomes likely requires rapid removal of blood from the parenchyma and restraining edema formation while also limiting further neuronal damage due to the surgical intervention. Minimally invasive surgery (MIS) approaches promise to provide these benefits and have become alluring options for management of ICH. This review describes six MIS techniques for ICH evacuation including craniopuncture, stereotactic aspiration with thrombolysis, endoport-mediated evacuation, endoscope-assisted evacuation, adjunctive aspiration devices, and the surgiscope. The efficacy of each modality is discussed based on current literature. The largest clinical trials have yet to demonstrate definitive effects of MIS intervention on mortality and functional outcomes for ICH. Thus, there is a significant need for further innovation for ICH treatment. Multiple ongoing trials promise to better clarify the potential of the newer, non-thrombolytic MIS techniques.
Analysis of Electrothermal Effects in Devices and Arrays in InGaP/GaAs HBT Technology
Authors: d’Alessandro Vincenzo , Catalano Antonio Pio , Scognamillo Ciro , Codecasa Lorenzo , Zampardi Peter J.
Published: March 2021

In this paper, the dc electrothermal behavior of InGaP/GaAs HBT test devices and arrays for power amplifier output stages is extensively analyzed through an efficient simulation approach. The approach relies on a full circuit representation of the domains, which accounts for electrothermal effects through the thermal equivalent of the Ohm’s law and can be solved in any commercial circuit simulator. In particular, the power-temperature feedback is described through an equivalent thermal network automatically obtained by (i) generating a realistic 3-D geometry/mesh of the domain in the environment of a numerical tool with the aid of an in-house routine; (ii) feeding the geometry/mesh to FANTASTIC, which extracts the network without performing simulations. Nonlinear thermal effects adversely affecting the behavior of devices/arrays at high temperatures are included through a calibrated Kirchhoff’s transformation. For the test devices, the thermally-induced distortion in I–V curves is explained, and the limits of the safe operating regions are identified for a wide range of bias conditions. A deep insight into the electrothermal behavior of the arrays is then provided, with particular emphasis on the detrimental nonuniform operation. Useful guidelines are offered to designers in terms of layout and choice of the ballasting strategy.
Enabling Parallelized-QEMU for Hardware/Software Co-Simulation Virtual Platforms
Authors: Díaz Edel , Mateos Raúl , Bueno Emilio J. , Nieto Rubén
Published: March 2021

Presently, the trend is to increase the number of cores per chip. This growth is appreciated in Multi-Processor System-On-Chips (MPSoC), composed of more cores in heterogeneous and homogeneous architectures in recent years. Thus, the difficulty of verification of this type of system has been great. The hardware/software co-simulation Virtual Platforms (VP) are presented as a perfect solution to address this complexity, allowing verification by simulation/emulation of software and hardware in the same environment. Some works parallelized the software emulator to reduce the verification times. An example of this parallelization is the QEMU (Quick EMUlator) tool. However, there is no solution to synchronize QEMU with the hardware simulator in this new parallel mode. This work analyzes the current software emulators and presents a new method to allow an external synchronization of QEMU in its parallelized mode. Timing details of the cores are taken into account. In addition, performance analysis of the software emulator with the new synchronization mechanism is presented, using: (1) a boot Linux for MPSoC Zynq-7000 (dual-core ARM Cortex-A9) (Xilinx, San Jose, CA, USA); (2) an FPGA-Linux co-simulation of a power grid monitoring system that is subsequently implemented in an industrial application. The results show that the novel synchronization mechanism does not add any appreciable computational load and enables parallelized-QEMU in hardware/software co-simulation virtual platforms.
Sustainable Architecture—What’s Next?
Authors: Røstvik Harald N.
Published: March 2021

Sustainable architecture encompasses more than energy efficiency, zero carbon dioxide (CO2) emission or renewable energy use in the built environment. It also needs to alleviate overall impacts on the natural environment or ecosystem that surrounds it. It may be argued that primitive vernacular architecture (architecture without architects) built and operated using local techniques and resources alone can be considered to be sustainable. Yet later, after the 1992 Rio Conference and its declarations, more specific definitions emerged putting weight on the rational use of land area, materials and energy, preferably local, as well as area efficient planning, economy and recyclability. The advantage of this is to reduce the ecological footprint of buildings and the climate gas emissions from a sector that represents 35–50 percent of global climate gas emissions, depending on how one counts. This paper clarifies concepts, questions cemented truths and points a way forward by asking; what’s next?
Automatic and Intelligent Technologies of Solid-State Fermentation Process of Baijiu Production: Applications, Challenges, and Prospects
Authors: Ye Hong , Wang Juan , Shi Jie , Du Jingyi , Zhou Yuanhao , Huang Mingquan , Sun Baoguo
Published: March 2021

Baijiu is the national liquor of China and the world’s most consumed spirit, which is produced using a unique and traditional solid-state fermentation (SSF) process. The development of an automatic and intelligent technology for SSF is more difficult than that for liquid-state fermentation. However, the technological upgrading of the SSF process is crucial for reducing the labor intensity, saving manpower, avoiding the waste of materials and energy, and providing a favorable operation environment for workers; moreover, it provides a great reference value to similar industries. This article reviews the latest application progresses in automatic and intelligent technologies for Baijiu production. The important technical processes are introduced successively, including the production of Jiuqu, SSF, solid-state distillation, storage, and blending. The bottlenecks and challenges are pointed out for automatic and intelligent upgrading of these technical processes. Furthermore, the typical technology application cases in an integrated automatic production line of Baijiu are also summarized. Next, the industrial development status of Baijiu production is compared with those of other liquors in the world. Finally, future development directions are proposed. This review will provide an overall introduction and objective analysis of recent developments and current challenges in Baijiu manufacturing so as to promote the intelligent brewing of Baijiu.
Understanding Food Waste, Food Insecurity, and the Gap between the Two: A Nationwide Cross-Sectional Study in Saudi Arabia
Authors: Althumiri Nora A. , Basyouni Mada H. , Duhaim Ali F. , AlMousa Norah , AlJuwaysim Mohammed F. , BinDhim Nasser F.
Published: March 2021

Background: Food waste and food insecurity may co-exist in various balances in developing and developed countries. This study aimed to explore the levels of food waste and food insecurity, the factors associated with them, and their relationships at the household and individual levels in Saudi Arabia. Methods: This study was a nationwide cross-sectional survey conducted via computer-assisted phone interviews in January 2021. Quota sampling was utilized to generate balanced distributions of participants by gender across all the administrative regions of Saudi Arabia. Data collection included household demographics, food waste and disposal, the Food Insecurity Experience Scale (FIES), and the Household Food Insecurity Access Scale (HFIAS). Results: Out of the 2807 potential participants contacted, 2454 (87.4%) completed the interview. The mean age was 31.4 (SD = 11.7; range = 18–99) and 50.1% were female. The weighted prevalence of uncooked food waste in the last four weeks was 63.6% and the cooked food waste was 74.4%. However, the food insecurity weighted prevalence at the individual level (FIES) was 6.8%. In terms of food insecurity at the household level (HFIAS), 13.3% were in the “severely food insecure” category. Moreover, this study found that “moderately food insecure” households were associated with an increased likelihood to waste uncooked food (relative risk (RR) = 1.25), and the “mildly food insecure” (RR = 1.21) and “moderately food insecure” (RR = 1.17) households were associated with an increased likelihood to waste cooked food. However, “food secure” households were associated with a decreased likelihood to waste cooked food (RR = 0.56). Finally, this study identified four household factors associated with food waste and three household factors that were associated with “severe food insecurity.” Conclusions: This first national coverage study to explore food waste and food insecurity at the individual level and household level, identified household factors associated with food waste and food insecurity and identified new associations between food waste and food insecurity in Saudi Arabia. The associations found between food waste and food insecurity are potential areas of intervention to reduce both food waste and food insecurity at the same time, toward achieving the Sustainable Development Goal (SDG) targets related to food waste and food security.
Does the ‘Mountain Pasture Product’ Claim Affect Local Cheese Acceptability?
Authors: Endrizzi Isabella , Cliceri Danny , Menghi Leonardo , Aprea Eugenio , Gasperi Flavia
Published: March 2021

This paper aims to explore the impact of “mountain pasture product” information on the acceptability of local protected designation of origin (PDO) cheese produced from the raw milk of cows grazing in mountain pastures (P) or reared in valley floor stalls (S). A total of 156 consumers (55% males, mean age 41 years) were asked to evaluate their overall liking on a 9-point hedonic scale of four samples: Cheeses P and S were presented twice with different information about the origin of the milk (cows grazing on mountain pasture or reared in a valley floor stall). Demographics, consumer habits, and opinions on mountain pasture practice (MPP), attitudes towards sustainability, and food-related behaviours (i.e., diet, food waste production, organic food, and zero food miles products purchase) were recorded and used to segment consumers. The cheeses were all considered more than acceptable, even though they were found to be significantly different in colour and texture by instrumental analyses. In the whole consumer panel, the cheese P was preferred, while in consumer segments less attentive to product characteristics, this effect was not significant. External information had a strong effect: Overall liking was significantly higher in cheeses presented as “mountain pasture product”, both in the whole panel and in consumer segments with different attitudes (except for those with a low opinion of MPP).
Consumer-Led Adaptation of the EsSense Profile® for Herbal Infusions
Authors: Rocha Célia , Pinto Moura Ana , Pereira Diana , Costa Lima Rui , Cunha Luís Miguel
Published: March 2021

This work aimed to adapt the EsSense Profile® emotions list to the discrimination of herbal infusions, aiming to evaluate the effect of harvesting conditions on the emotional profile. A panel of 100 consumers evaluated eight organic infusions: lemon verbena, peppermint, lemon thyme, lemongrass, chamomile, lemon balm, globe amaranth and tutsan, using a check-all-that-apply (CATA) ballot with the original EsSense Profile®. A set of criteria was applied to get a discriminant list. First, the terms with low discriminant power and with a frequency mention below 35% were removed. Two focus groups were also performed to evaluate the applicability of the questionnaire. The content analysis of focus groups suggests the removal of the terms good and pleasant, recognized as sensory attributes. Six additional terms were removed, considered to be too similar to other existing emotion terms. Changes in the questionnaire, resulting in a list of 24 emotion terms for the evaluation of selected herbal infusions, were able to discriminate beyond overall liking. When comparing finer differences between plants harvested under different conditions, differences were identified for lemon verbena infusions, yielding the mechanical cut of plant tips as the one leading to a more appealing evoked emotions profile.
Relationship between Sleep Duration Trajectories and Self-Rated Depressive Symptoms in South Koreans with Physical Disabilities
Authors: Yi Su Jeong , Jeong Yoo Mi , Kim Jae-Hyun
Published: March 2021

Physically disabled persons can have sleep problems, which affects their mental health more than those in non-disabled people. However, there are few studies on the relationship between sleep duration and mental health targeting physically disabled people in South Korea, and existing studies on the disabled have mostly used data collected from convenience rather than nationally representative samples, limiting the generalization of the results. This study used data from the second wave of the Panel Survey of Employment for the Disabled (PSED, 2016–2018, 1st–3rd year). Participants included 1851 physically disabled individuals. The Chi-square test and generalized estimating equation (GEE) were used and the Akaike information criterion (AIC) value and the AIC log Bayes factor approximation were used to select sleep trajectories. This is the first study to elucidate multiple sleep trajectories in physically disabled people in Korea, and the relationship between sleep duration trajectories and self-rated depressive symptoms. People with physical disabilities who sleep more than 9 h have the highest risk of depression and need more intensive management as a priority intervention.
Monitoring Vegetation Greenness in Response to Climate Variation along the Elevation Gradient in the Three-River Source Region of China
Authors: Wang Zhaoqi , Liu Xiang , Wang Hao , Zheng Kai , Li Honglin , Wang Gaini , An Zhifang
Published: March 2021

The Three-River Source Region (TRSR) is vital to the ecological security of China. However, the impact of global warming on the dynamics of vegetation along the elevation gradient in the TRSR remains unclear. Accordingly, we used multi-source remote sensing vegetation indices (VIs) (GIMMS (Global Inventory Modeling and Mapping Studies) LAI (Leaf Area Index), GIMMS NDVI (Normalized Difference Vegetation Index), GLOBMAP (Global Mapping) LAI, MODIS (Moderate Resolution Imaging Spectroradiometer) EVI (Enhanced Vegetation Index), MODIS NDVI, and MODIS NIRv (near-infrared reflectance of vegetation)) and digital elevation model data to study the changes of VGEG (Vegetation Greenness along the Elevation Gradient) in the TRSR from 2001 to 2016. Results showed that the areas with a positive correlation of vegetation greenness and elevation accounted for 36.34 ± 5.82% of the study areas. The interannual variations of VGEG showed that the significantly changed regions were mainly observed in the elevation gradient of 4–5 km. The VGEG was strongest in the elevation gradient of 4–5 km and weakest in the elevation gradient of >5 km. Correlation analysis showed that the mean annual temperature was positively correlated with VIs, and the effect of the mean annual precipitation on VIs was more obvious at low altitude than in high altitude. This study contributes to our understanding of the VGEG variation in the TRSR under global climate variation and also helps in the prediction of future carbon cycle patterns.
Single-Cell Transcriptomics Supports a Role of CHD8 in Autism
Authors: Hoffmann Anke , Spengler Dietmar
Published: March 2021

Chromodomain helicase domain 8 (CHD8) is one of the most frequently mutated and most penetrant genes in the autism spectrum disorder (ASD). Individuals with CHD8 mutations show leading symptoms of autism, macrocephaly, and facial dysmorphisms. The molecular and cellular mechanisms underpinning the early onset and development of these symptoms are still poorly understood and prevent timely and more efficient therapies of patients. Progress in this area will require an understanding of “when, why and how cells deviate from their normal trajectories”. High-throughput single-cell RNA sequencing (sc-RNAseq) directly quantifies information-bearing RNA molecules that enact each cell’s biological identity. Here, we discuss recent insights from sc-RNAseq of CRISPR/Cas9-editing of Chd8/CHD8 during mouse neocorticogenesis and human cerebral organoids. Given that the deregulation of the balance between excitation and inhibition (E/I balance) in cortical and subcortical circuits is thought to represent a major etiopathogenetic mechanism in ASD, we focus on the question of whether, and to what degree, results from current sc-RNAseq studies support this hypothesis. Beyond that, we discuss the pros and cons of these approaches and further steps to be taken to harvest the full potential of these transformative techniques.
Identification and Expression Characterization of ATP-Binding Cassette (ABC) Transporter Genes in Melon Fly
Authors: Xu Hui-Qian , Ma Meng , Ma Yun-Peng , Zhang Su-Yun , Li Wei-Jun , Wei Dong , Wang Jin-Jun
Published: March 2021

The ATP-binding cassette (ABC) transporter is a protein superfamily that transports specific substrate molecules across lipid membranes in all living species. In insects, ABC transporter is one of the major transmembrane protein families involved in the development of xenobiotic resistance. Here, we report 49 ABC transporter genes divided into eight subfamilies (ABCA-ABCH), including seven ABCAs, seven ABCBs, 10 ABCCs, two ABCDs, one ABCE, three ABCFs, 16 ABCGs, and three ABCHs according to phylogenetic analysis in Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. The expressions level of 49 ABC transporters throughout various developmental stages and within different tissues were evaluated by quantitative transcriptomic analysis, and their expressions in response to three different insecticides were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). These ABC transporter genes were widely expressed at developmental stages but most highly expressed in tissues of the midgut, fat body and Malpighian tube. When challenged by exposure to three insecticides, abamectin, β-cypermethrin, and dinotefuran, the expressions of ZcABCB7 and ZcABCC2 were significantly up-regulated. ZcABCB1, ZcABCB6, ZcABCB7, ZcABCC2, ZcABCC3, ZcABCC4, ZcABCC5, and ZcABCC7 were significantly up-regulated in the fat body at 24 h after β-cypermethrin exposure. These data suggest that ZcABCB7 and ZcABCC2 might play key roles in xenobiotic metabolism in Z. cucurbitae. Collectively, these data provide a foundation for further analysis of ABCs in Z. cucurbitae.
Differential Gene Expression in the Heads of Behaviorally Divergent Culex pipiens Mosquitoes
Authors: Noreuil Anna , Fritz Megan L.
Published: March 2021

Host preferences of Cx. pipiens, a bridge vector for West Nile virus to humans, have the potential to drive pathogen transmission dynamics. Yet much remains unknown about the extent of variation in these preferences and their molecular basis. We conducted host choice assays in a laboratory setting to quantify multi-day human and avian landing rates for Cx. pipiens females. Assayed populations originated from five above-ground and three below-ground breeding and overwintering habitats. All three below-ground populations were biased toward human landings, with rates of human landing ranging from 69–85%. Of the five above-ground populations, four had avian landing rates of >80%, while one landed on the avian host only 44% of the time. Overall response rates and willingness to alternate landing on the human and avian hosts across multiple days of testing also varied by population. For one human- and one avian-preferring population, we examined patterns of differential expression and splice site variation at genes expressed in female heads. We also compared gene expression and splice site variation within human-seeking females in either gravid or host-seeking physiological states to identify genes that may regulate blood feeding behaviors. Overall, we identified genes with metabolic and regulatory function that were differentially expressed in our comparison of gravid and host-seeking females. Differentially expressed genes in our comparison of avian- and human-seeking females were enriched for those involved in sensory perception. We conclude with a discussion of specific sensory genes and their potential influence on the divergent behaviors of avian- and human-seeking Cx. pipiens.
Reduced Flow-Mediated Dilatation Is Not Related to COVID-19 Severity Three Months after Hospitalization for SARS-CoV-2 Infection
Authors: Riou Marianne , Oulehri Walid , Momas Cedric , Rouyer Olivier , Lebourg Fabienne , Meyer Alain , Enache Irina , Pistea Cristina , Charloux Anne , Marcot Christophe , de Blay Frederic , Collange Olivier , Mertes Michel , Andrès Emmanuel , Talha Samy , Geny Bernard
Published: March 2021

The coronavirus disease 2019 (COVID-19) pandemic has spread rapidly worldwide, with more than two million deaths. Evidence indicates the critical role of the vascular endothelium in its pathophysiology but, like potential changes in functional vasodilation, the vascular effect of SARS-CoV-2 at a given distance from the acute infection is largely unknown. We assessed brachial artery flow-mediated dilatation (FMD) in 27 COVID-19 patients needing conventional or intensive care unit hospitalization, three months after SARS-CoV-2 infection diagnosis and in nine age- and sex- matched control subjects. Interestingly, the FMD was lower in COVID-19 patients as compared to controls (8.2 (7.2–8.9) vs. 10.3 (9.1–11.7)); p = 0.002, and half of the hospitalized COVID-19 survivors presented with a reduced FMD < 8% at three months of COVID-19 onset. Impaired FMD was not associated with severe or critical SARS-CoV-2 infection, reflected by ICU hospitalization, total hospitalization duration, or severity of lung damage. In conclusion, reduced FMD is often observed even three months after hospitalization for SARS-CoV-2 infection, but such alteration predominantly appears to not be related to COVID-19 severity. Longer and larger follow-up studies will help to clarify the potential prognosis value of FMD among COVID-19 patients, as well as to further determine the mechanisms involved.
Association of Barriers, Fear of Falling and Fatigue with Objectively Measured Physical Activity and Sedentary Behavior in Chronic Stroke
Authors: Sánchez-Sánchez M. Luz , Arnal-Gómez Anna , Cortes-Amador Sara , Pérez-Alenda Sofía , Carrasco Juan J. , Climent-Toledo Assumpta , Espí-López Gemma Victoria , Ruescas-Nicolau Maria-Arantzazu
Published: March 2021

Understanding the fostering factors of physical activity (PA) and sedentary behavior (SB) in post-stroke chronic survivors is critical to address preventive and health interventions. This cross-sectional study aimed to analyze the association of barriers to PA, fear of falling and severity of fatigue encountered by stroke chronic survivors with device-measured PA and SB. Ambulatory community-dwelling post-stroke subjects (≥six months from stroke onset) were evaluated and answered the Barriers to Physical Activity after Stroke Scale (BAPAS), Short Falls Efficacy Scale-International (Short FES-I) and Fatigue Severity Scale (FSS). SB and PA were measured with an Actigraph GT3X+ accelerometer for ≥seven consecutive days. Stepwise multiple linear regression analysis was employed to identify factors associated with PA and SB. Fifty-seven participants (58.2 ± 11.1 years, 37 men) met the accelerometer wear–time criteria (three days, ≥eight h/day). The physical BAPAS score explained 28.7% of the variance of the prolonged sedentary time (β = 0.547; p < 0.001). Additionally, the walking speed (β = 0.452) together with physical BAPAS (β = −0.319) explained 37.9% of the moderate-to-vigorous PA time (p < 0.001). In chronic post-stroke survivors, not only the walking speed but, also, the perceived physical barriers to PA are accounted for the SB and PA. Interventions to reverse SB and to involve subjects post-stroke in higher levels of PA should consider these factors.
Ferric Carboxymatose in Non-Hemodialysis CKD Patients: A Longitudinal Cohort Study
Authors: Minutolo Roberto , Berto Patrizia , Liberti Maria Elena , Peruzzu Nicola , Borrelli Silvio , Netti Antonella , Garofalo Carlo , Conte Giuseppe , De Nicola Luca , Del Vecchio Lucia , Locatelli Francesco
Published: March 2021

No information is available on the efficacy of ferric carboxymaltose (FCM) in real-world CKD patients outside the hemodialysis setting. We prospectively followed 59 non-hemodialysis CKD patients with iron deficient anemia (IDA: hemoglobin <12.0/<13.5 g/dL in women/men and TSAT < 20% and/or ferritin < 100 ng/mL) who were intolerant or non-responders to oral iron. Patients received ferric carboxymaltose (FCM) (single dose of 500 mg) followed by additional doses if iron deficiency persisted. We evaluated efficacy of FCM in terms of increase of hemoglobin, ferritin, and TSAT levels. Direct and indirect costs of FCM were also analyzed in comparison with a hypothetical scenario where same amount of iron as ferric gluconate (FG) was administered intravenously. During the 24 weeks of study, 847 ± 428 mg of FCM per patient were administered. IDA improved after four weeks of FCM and remained stable thereafter. At week-24, mean change (95%CI) from baseline of hemoglobin, ferritin and TSAT were +1.16 g/dL (0.55–1.77), +104 ng/mL (40–168) and +9.5% (5.8–13.2), respectively. These changes were independent from ESA use and clinical setting (non-dialysis CKD, peritoneal dialysis and kidney transplant). Among ESA-treated patients (n = 24), ESA doses significantly decreased by 26% with treatment and stopped either temporarily or persistently in nine patients. FCM, compared to a FG-based scenario, was associated with a cost saving of 288 euros/patient/24 weeks. Saving was the same in ESA users/non-users. Therefore, in non-hemodialysis CKD patients, FCM effectively corrects IDA and allows remarkable cost savings in terms of societal, healthcare and patient perspective.