bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity Analysis of Macrofungi and Lichenised Fungi in Pyrenean Oak (Quercus pyrenaica Willd.) and Chestnut (Castanea sativa L.) Forests: Implications for the Conservation of Forest Habitats in Castilla y León (Central-Northwest Spain)
Authors: Rodríguez-de la Cruz David , Perfecto-Arribas Sonia , Delgado-Sánchez Luis
Published: December 2024

Fungi can be used as bioindicators to assess the biodiversity and conservation status of different habitats because of their high adaptability and sensitivity to changes in water, air, and soil quality. In this study, records of macrofungi and lichenised fungi were extracted from GBIF, surveyed using GIS software, and used to compare the fungal diversity of the Pyrenean oak and chestnut groves of Castilla y León, analysing the possible implications of their presence for the conservation of these forest habitats. In Quercus pyrenaica forests, a greater number of lichen and macrofungi species and records were recorded than in Castanea sativa forests, although the greater area occupied by the former could have influenced this diversity. The higher presence of ectomycorrhizal macrofungal species in chestnut groves, as well as the higher sensitivity to pollution of lichens in Pyrenean oak-dominated environments, showed the relevance of the analysis of these data for a better understanding of the conservation status of forest habitats. However, in order to obtain more accurate results, it would be necessary to carry out specific studies on a smaller scale.
Resilience of the Miombo Woodland to Different Fire Frequencies in the LevasFlor Forest Concession, Central Mozambique
Authors: Meneses Osvaldo M. , Ribeiro Natasha S. , Shirvani Zeinab , Andrew Samora M.
Published: December 2024

Fires play a significant role in shaping the Miombo woodlands. Understanding how fire affects the Miombo region’s resilience is crucial for ensuring its sustainability. This study evaluated plant composition and structure across different fire frequencies in the Miombo woodlands of the LevasFlor Forest Concession (LFC), central Mozambique. Fire frequency clusters-high (HFF), moderate (MFF), and low (LFF)-were identified using a 21-year remote-sensing dataset. In each cluster, 90 random sampling plots were established (30 per cluster). In each plot, the diameter at breast height (DBH) and total height of the saplings and trees were measured. Subplots were used to count and identify seedlings, herbs, climbers, and grasses. Plant species richness, evenness,—diversity, the importance value index (IVI), and similarity were computed to assess plant composition. For the structure, stem density, biomass, basal area, diameter, and height were assessed. A total of 124 plant species-including trees, saplings, seedlings, herbs, climbers, and grasses-were identified across the three clusters. The Bray-Curtis Dissimilarity Index, tested with an ANOSIM similarity test, revealed significant differences in plant species composition among clusters (p < 0.0003), with an overall average dissimilarity of 71.98%. In the HFF cluster, fire-tolerant species were among the five species with the highest IVI, while fire-sensitive species predominated in the LFF. Additionally, the Kruskal-Wallis test indicated significant differences in seedling stem density (p < 0.005) between the LFF and other clusters. However, overall, the composition and structure attributes suggested that current fire regime does not significantly compromise the plant species resilience of the Miombo woodlands in the LFC. Still, it is essential to concentrate management and conservation efforts on seedlings of some key Miombo species, such as Brachystegia spiciformis, whose ecology is particularly affected by fire.
A Comparative Survey of Centralised and Decentralised Identity Management Systems: Analysing Scalability, Security, and Feasibility
Authors: Goel Aviral , Rahulamathavan Yogachandran
Published: December 2024

Traditional identity management (IdM) solutions based on centralised protocols, such as Lightweight Directory Access Protocol (LDAP) and Security Assertion Markup Language (SAML), are where a central authority manages all the processes. This risks a single point of failure and other vulnerabilities. In response, decentralised techniques like blockchain and decentralised identities (DIDs) are being explored. This review paper performs a comparison of popular decentralised identity management (DIM) protocols, such as self-sovereign identity (SSI), against traditional centralised approaches such as LDAP and SAML. These decentralised identity management systems are being developed, keeping users’ identity data as its highest priority. Additionally, this method eliminates the need for a central authority to manage and secure the system. To further explore the potential of decentralised identity management, this study delves into popular blockchain-based decentralised identity management systems such as uPort, Sovrin, EverID, Blockstack, ShoCard, and Hyperledger Indy. We analyse their underlying principles and compare them with the well-established centralised identity management solutions, focusing on key aspects such as scalability, security, and feasibility. However, despite their benefits and several worthy developments in this field, decentralised approaches are still not widely used. Through this study, we investigate both centralised and decentralised methods and review their strengths and weaknesses. By reviewing multiple research papers, this survey aims to provide an understanding and aid in selecting the most suitable identity management system for different use cases.
Impact of Addition of a Newtonian Solvent to a Giesekus Fluid: Analytical Determination of Flow Rate in Plane Laminar Motion
Authors: Daprà Irene , Scarpi Giambattista , Di Federico Vittorio
Published: December 2024

In this study, the influence of the presence of a Newtonian solvent on the flow of a Giesekus fluid in a plane channel or fracture is investigated with a focus on the determination of the flow rate for an assigned external pressure gradient. The pressure field is nonlinear due to the presence of the normal transverse stress component. As expected, the flow rate per unit width Q′ is larger than for a Newtonian fluid and decreases as the solvent increases. It is strongly dependent on the viscosity ratio ε (0≤ε≤1), the dimensionless mobility parameter β (0≤β≤1) and the Deborah number De, the dimensionless driving pressure gradient. The degree of dependency is notably strong in the low range of ε. Furthermore, Q′ increases with De and tends to a constant asymptotic value for large De, subject to the limitation of laminar flow. When the mobility factor β is in the range 0.5÷1, there is a minimum value of ε  to obtain an assigned value of De. The ratio UN/U between Newtonian and actual mean velocity depends only on the product βDe, as for other non-Newtonian fluids.
The Effect of Salt Reduction on the Microbial Community Structure and Metabolite Composition of Cheddar Cheese
Authors: Wang Xinping , Kang You , Gao Lei , Zhao Yujuan , Gao Yansong , Yang Ge , Li Shengyu
Published: December 2024

As consumer demand for low-salt diets increases, the development of low-salt cheese has emerged as a prevailing trend. To gain a deeper insight into the effects of salt reduction on cheddar cheese, this study used cheddar cheese with a 2.0% salt concentration (full salt, FS) as the standard control, exploring the differences in quality and composition between cheddar cheese with a 1.5% salt concentration (reduced salt, RS) and a 1.0% salt concentration (half salt, HS). The results revealed that, while the RS group exhibited significant differences in texture compared to the FS group, their physicochemical composition and microbial communities were similar, resulting in a product with quality comparable to traditional cheese. In contrast, the HS group differed notably from the FS group in terms of its physicochemical composition, texture, and microbial communities. Shifts in the microbial community within the HS group promoted enhanced protein metabolism, producing a substantial increase in free amino acids and volatile flavor compounds. In summary, cheddar cheese with a 1.5% salt concentration is similar to traditional varieties in terms of quality, while the 1.0% salt variety displays a more complex composition, due to microbial community shifts facilitating protein metabolism.
Assessment and Prediction of the Food Production Capacity and Consumption Gap in Arid Oasis Metropolitan Area
Authors: Cui Mingjie , Zhang Yufang , Yang Degang , Xu Wenqiang , Lu Rongqian , Bai Wenshuai
Published: December 2024

Food security is the foundation of sustainable human development, and the balance between food supply and demand in urban areas is highly important for promoting residents’ health and the sustainable development of cities. This paper takes the Urumqi Metropolitan Area (UMA), a typical oasis urban area, as the study area and uses the food production—demand gap indicator to assess the balance of food production and consumption in the region from 2000 to 2020 and projects food demand in 2030–2060. The results show, first, that residents’ food consumption is characterized by high carbohydrate, protein, and fat consumption, and that this put more pressure on food production. Second, different food consumption structures will have different impacts on food production, and the local food production capacity in UMA falls short of ensuring a balanced nutritional structure for residents. Third, food demand increases significantly in 2030–2060, and the pressure of population consumption structure on food production is much greater than that of population growth. Considering the environmental effects of food transportation and the loss of food nutrients, on the production side, the construction of the UMA should be accelerated by including Qitai County and Jimsar County in the UMA’s planning scope, strengthening city—regional connections, and improving the local food production and supply capacity of surrounding areas. On the consumption side, regional dietary guidelines should be developed based on local dietary culture and agricultural production conditions to help guide residents to adjust their dietary structures, thereby alleviating pressure on local food demand. Such measures are crucial for ensuring sufficient food supply and promoting balanced nutrition among the population.
Differences in the Quality Components of Wuyi Rock Tea and Huizhou Rock Tea
Authors: Wu Zhaobao , Liao Weiwen , Zhao Hongbo , Qiu Zihao , Zheng Peng , Liu Yuxuan , Lin Xinyuan , Yao Jiyuan , Li Ansheng , Tan Xindong , Sun Binmei , Meng Hui , Liu Shaoqun
Published: December 2024

Different origins and qualities can lead to differences in the taste and aroma of tea; however, the impacts of origin and quality on the taste and aroma characteristics of Wuyi rock tea and Huizhou rock tea have rarely been studied. In this study, high-performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC–MS), and sensory evaluation methods were used to compare the quality components of Wuyi rock tea and Huizhou rock tea. The sensory evaluation showed that they each have their own characteristics, but the overall acceptability of Wuyi rock tea is ahead of Huizhou rock tea (p < 0.01). Biochemical experiments showed that HT was the highest in water leachables, about 43.12%; WT was the highest in tea polyphenols, about 14.91%; WR was the highest in free amino acids, about 3.38%; and the six rock teas had different health benefits. High-performance liquid chromatography showed that the theanine contents of WS and WR were 0.183% and 0.103%, respectively, which were much higher than those of other varieties. The OPLS-DA model predicted the factors that caused their different tastes, in order of contribution: CG > ECG > caffeine > EGCG > theanine. Ten volatile substances with OAV ≥ 1 and VIP > 1 were also found, indicating that they contributed greatly to the aroma characteristics, especially hexanoic acid, hexyl ester, and benzyl nitrile. The results of the correlation analysis showed that theanine was significantly correlated with taste (p < 0.05), and hexanoic acid, hexyl ester, and benzyl nitrile were significantly correlated with smell (p < 0.05). Substances such as theanine, hexanoic acid, hexyl ester, and benzyl nitrile give them their unique characteristics. Analysis of the differences in the quality components of the six rock teas can provide reference value for the cultivation and processing of rock teas.
Comparative Investigation of Untargeted and Targeted Metabolomics in Turmeric Dietary Supplements and Rhizomes
Authors: Singh Jashbir , Tareq Fakir Shahidullah , Luthria Devanand L.
Published: December 2024

In the present study, we analyzed the bioactive curcuminoids content in eight capsules (DS-1-DS-7 and DS-9), one tablet (DS-8), three ground turmeric samples (DS-10-DS-12), and three ground turmeric rhizomes (TR-1, TR-2, and TR-3). Initial screening with infrared and ultraviolet–visible spectroscopy coupled with a principal component analysis (PCA) revealed distinct differences between the samples analyzed. Hence, targeted and untargeted analyses were performed using ultra-high-performance liquid chromatography and gas chromatography coupled with mass spectrometry detections. The results show that the total curcuminoids content ranged from 1.3 to 69.8 mg/100 mg and the volatile component ranged from 0.7 to 9.1 mg/100 mg. The percentage ratio of the three prominent curcuminoids, bisdesmethoxycurcumin (BMC), desmethoxycurcumin (DMC), and curcumin (CUR), also varied remarkably compared to the expected ratio (BMC:DMC:CUR ratio of 1:2:6) described in the literature. The three prominent volatile compounds identified in most samples were ar-turmerone, turmerone, and curlone. The results demonstrated significant differences in the volatile compound levels among the DS and dried rhizome samples. The non-targeted analysis resulted in the identification of over 40 compounds, including bioactives such as piperine, phenolic acids, and amino acids. A disintegration study was performed on limited DS according to the United States Pharmacopeia protocol. The results reveal that all the selected DS samples passed the disintegration test. An analysis of curcuminoids from DS samples in neutral and acidic solutions demonstrated that all curcuminoids (BMC, DMC, and CUR) existed in the keto and enol forms and their concentrations changed with pH. This study will be of significant interest to manufacturers, consumers, and pharmacologists to accurately understand the bioactivities of three curcuminoids in different isomeric forms.
A Bicomplex Proportional Fractional (ϑ,φ)-Weighted Cauchy–Riemann Operator Using Riemann–Liouville Derivatives with Respect to an Hyperbolic-Valued Function
Authors: González-Cervantes José Oscar , Bory-Reyes Juan , Ramírez-Belman Juan Adrián
Published: December 2024

Based on the Riemann–Liouville derivatives with respect to functions taking values in the set of hyperbolic numbers, we consider a new bicomplex proportional fractional (ϑ,φ)-weighted Cauchy–Riemann operator, involving orthogonal bicomplex functions as weights, and its associated fractional Borel–Pompeiu formula is proved as the main result.
Enhanced Thermal Safety of Hydrophobic SiO2 Aerogels Through Introduction of Layered Double Oxides
Authors: Xu Lei , Sun Guanhua , Chen Jiahui , Wu Xiaoxu , Hu Min , Zhou Fang , Li Zhi
Published: December 2024

This research enhances the thermal safety of hydrophobic silica aerogel (HSA) by integrating layered double oxides (LDOs). XRD and FTIR confirm that the introduction of LDOs does not affect the formation of SA. The LDO/SA composites demonstrate a low density (0.14–0.16 g/cm3), low thermal conductivity (23.28–28.72 mW/(m·K)), high porosity (93.4–96.1%), and a high surface area (899.2–1006.4 m2/g). The TG-DSC results reveal that LDO/SA shows enhanced thermal stability, with increases of 49 °C in the decomposition onset temperature and 47.4 °C in the peak decomposition temperature. The gross calorific value of LDO/SA-15% (with 15 wt% LDO) exhibits a 23.9% reduction in comparison to that of pure SA. The decrease in gross calorific value, along with improved thermal stability, indicates a boost in the thermal safety characteristics of the LDO/SA composites. This study demonstrates that incorporating LDOs enhances the thermal safety of HSA, while preserving its superior performance, thus broadening its potential applications in thermal insulation.
Processes of Obtaining Nanostructured Materialswith a Hierarchical Porous Structure on the Example of Alginate Aerogels
Authors: Menshutina Natalia , Fedotova Olga , Abramov Andrey , Golubev Eldar , Sulkhanov Yan , Tsygankov Pavel
Published: December 2024

Currently, materials with specific, strictly defined functional properties are becoming increasingly important. A promising strategy for achieving these properties involves developing methods that facilitate the formation of hierarchical porous materials that combine micro-, meso-, and macropores in their structure. Macropores facilitate effective mass transfer of substances to the meso- and micropores, where further adsorption or reaction processes can occur. Aerogels represent a promising class of materials for implementing this approach. The formation of hierarchical porous structures in aerogels can be achieved using soft and hard templating methods or by foaming techniques. This paper presents a comprehensive study of three methods for forming hierarchical porous structures in alginate aerogels: (1) employing surfactants (Pluronic F-68), (2) using zein as a pore-forming component, and (3) foaming in a carbon dioxide medium. The results of micro-CT showed that each of the methods contributes to the formation of macropores within the structure of the resulting aerogels. Size distribution curves of the detected macropores were obtained, showing the presence of macropores ranging from 16 to 323 μm in size for samples obtained using surfactants, from 5 to 195 μm for samples obtained using zein, and from 20 μm to 3 mm for samples obtained by foaming in a carbon dioxide medium. The SEM images demonstrated the macro- and mesoporous fibrous structure of the obtained materials. The nitrogen porosimetry results indicated that samples obtained using surfactants and zein are characterized by a high specific surface area (592–673 m2/g), comparable to the specific surface area for an alginate-based aerogel obtained without the use of pore-forming components. However, the use of the developed methods for the formation of a hierarchical porous structure contributes to an increase in the specific mesopores volume (up to 17.7 cm3/g). The materials obtained by foaming in a carbon dioxide medium are characterized by lower specific surface areas (112–239 m2/g) and specific mesopores volumes (0.6–2.1 cm3/g). Thus, this paper presents a set of methods for forming hierarchical porous structures that can obtain delivery systems for active substances with a controlled release profile and highly efficient platforms for cell culturing.
Adoption Agrafa, Parts “Unwritten” About Cold War Adoptions from Greece
Authors: Van Steen Gonda A. H.
Published: December 2024

This preliminary empirical study delves into the “agrafa”, the “unwritten” or “uncharted” parts of a Greek adoption phenomenon and Greek–American relations that may, however, still be accessed via archival investigations, mixed research methods, and efforts to hone life writing skills. At stake is the case of the post-WWII adoptions of some 4000 Greek children who were sent to the United States between the years 1950 and 1975. This study asks how the related negotiations were transacted, especially in the early years of the intercountry adoption phenomenon. It challenges the researcher today to create a life writing narrative out of scant snippets and dense allusions and to disclose the dynamics of overlooked interactions, such as the consumerist and occasionally racist attitudes of some, though certainly not all, prospective adoptive parents. Thus, this article highlights formerly dismissed interactions, not necessarily numerically representative interactions, given that the window of opportunity has passed to interview adoptive parents of Greek children who pursued these foreign adoptions in the 1950s–1960s and to quantify their actions and reactions more systematically. Many of the adoptive parents of the 1950s–1960s, however, left their impressions, demands, and frustrations in writing. Those writings have yet to be studied, and their more deliberate, explicit language must be acknowledged, even amid generally more positive depictions of postwar intercountry adoption. I show that the victorious post-WWII era saw a sense of American entitlement emerge among the prospective adoptive parents that has since been whitewashed. Waiving the banner of altruism or humanitarianism (as a couple or as a superpower, respectively), some adoptive parents embarked on adoptions from Greece from a position of cultural as well as political and economic superiority. Their expectation was that the “destitute” partner should comply, that the Greeks themselves should not “talk back” when “poor orphans” were about to be “saved” from “illegitimacy” and lack of prospects.
Comparing Quality of Life and Psychological Changes in Benign and Malignant Lung Resections
Authors: Nicola Alin , Adelina Mavrea , Porosnicu Tamara Mirela , Oancea Cristian , Marc Monica Steluta , Barata Paula Irina
Published: December 2024

Background and Objectives: Pulmonary resections are critical interventions for treating various lung pathologies, both benign and malignant. Understanding the impact of these surgeries on patients’ Quality of Life (QoL) is essential for optimizing care. This study aims to compare the Health-Related Quality of Life (HRQoL) and psychological well-being in patients who underwent pulmonary resections for benign versus malignant etiologies. Methods: A cross-sectional study was conducted involving 117 patients who underwent pulmonary resection between January 2022 and June 2023. Participants were divided into two groups: 51 patients with benign lung conditions and 66 with malignant lung tumors. HRQoL was assessed using the SF-36 and WHOQOL-BREF questionnaires. Anxiety and depression levels were evaluated using the Hospital Anxiety and Depression Scale (HADS) and the Perceived Stress Scale (PSS-10). Patients were assessed pre- and post-intervention. Results: Patients with malignant etiologies were older (58.7 vs. 54.2 years) and had lower FEV1% predicted (79.1% vs. 82.5%) compared to the benign group. Malignant patients reported significantly lower scores in physical functioning (68.1 vs. 75.4), role-physical (65.0 vs. 72.3), and general health domains of the SF-36 (62.4 vs. 70.2). WHOQOL-BREF scores indicated a lower overall QoL in the malignant group, particularly in the physical health (65.3 vs. 72.1) and psychological domains (68.0 vs. 74.5). HADS scores revealed higher anxiety (9.1 vs. 7.2) and depression levels (8.5 vs. 6.8) among malignant patients. Correlation analyses showed strong associations between lower QoL scores and higher anxiety and depression levels. Conclusions: Pulmonary resections for malignant conditions are associated with a significant decline in HRQoL compared to benign conditions. Patients with malignant etiologies experience higher levels of anxiety and depression, emphasizing that clinicians should integrate specialized mental health services and tailored physical rehabilitation programs for patients undergoing pulmonary resections for malignant lung conditions to address their significantly reduced quality of life and increased psychological distress.
Depression, Anxiety, and Stress Symptoms (DASS-21) in Elderly Women in Association with Health Status (SHSQ-25): A Cross-Sectional Study
Authors: Beniusiene Asta , Kontautiene Vyte , Strukcinskiene Birute , Grigoliene Rasa , Martisauskiene Dalia , Jurgaitis Jonas
Published: December 2024

Background/Objectives: International studies state that older adults are at an increased risk of mental health symptoms and disorders (depression, anxiety, and stress), especially elderly women aged >65 years. The literature on this topic is scarce, and there is a need for studies that investigate the associations between mental health issues and overall health in elderly women. This study aimed to investigate depression, anxiety, and stress symptoms in elderly women in association with health status. Methods: A community-based cross-sectional study was conducted in Klaipeda, Lithuania, in 2020. The survey included elderly women (n = 306) aged 65 to 80 years studying at the Third Age University in Klaipeda city. The DASS-21 and SHSQ-25 scales were used for the survey. Associations and correlations between both scale indicators were calculated. Results: Sociodemographic factors such as age, marital status, and place of residence were not statistically significant in terms of mental health, except for women living alone, who had slightly lower levels of depressive symptoms (p = 0.015). Mental health issues were quite common; almost half of the participants had higher than normal levels of anxiety (49.0%) and depression (48.4%), and one-third (29.8%) experienced stress. A strong positive correlation was observed between mental health symptoms and physical health, with anxiety having the greatest effect size on fatigue (Cohen’s d = 0.950, p < 0.001), cardiovascular symptoms (Cohen’s d = 0.757, p < 0.001), and mental status (Cohen’s d = 1.036, p < 0.001). Depressive symptoms had a large effect size on fatigue (Cohen’s d = 0.764, p < 0.001) and mental status (Cohen’s d = 0.816, p < 0.001), while stress had a moderate effect size on all health domains. Conclusions: The findings highlight that anxiety symptoms are a major contributor to mental health conditions and overall health in elderly women. This study’s results emphasise the need for targeted interventions to address mental health challenges in elderly women.
Evolution of Physical Self-Esteem During Pulmonary Rehabilitation in Patients with Chronic Obstructive Pulmonary Disease: An Observational Study
Authors: Beaumont Marc , Mercier Arthur , Péran Loic , Berriet Anne Cécile , Le Ber Catherine , Reychler Gregory
Published: December 2024

Background/Objectives: Patients with COPD have altered self-esteem, and good self-esteem promotes personal, health, and social success. Improving self-esteem could be a method for encouraging the maintenance of physical activity. Only one study has evaluated the effects of pulmonary rehabilitation (PR) on self-esteem in moderate COPD patients. The objective was to assess the evolution of self-esteem in COPD patients of all stages of severity during PR. Methods: COPD patients undergoing PR were included in this prospective observational study. Patients were evaluated before and after the 4-week PR program. The objectives were to (1) assess the evolution of self-esteem using the Physical Self Inventory-6 questionnaire (PSI-6), (2) assess the evolution in each sub-score of PSI-6, (3) examine the correlations between the evolution of self-esteem and the evolution of parameters usually used during PR, and (4) determine an MID for self-esteem. Results: In total, 76 patients were included. We found that there was a significant increase in the total score in PSI-6 (9.29, p < 0.001), CI 95% [6.74; 11.83], and in the sub-scores of PSI-6. The evolution of the PSI-6 score was moderately correlated with changes in exercise capacity using STST1 (r = 0.352, p = 0.002) and quality of life (r = −0.361, p = 0.001) and weakly correlated to changes in dyspnea (r = −0.245, p = 0.03), anxiety (r = −0.248, p = 0.03), and depression (r = −0.290, p = 0.01). Using a distribution-based analysis, we found an MID between 5.2 and 5.6. Conclusions: We showed a significant increase in global score and in each sub-score of self-esteem using PSI-6 in COPD patients undergoing a PR program. We propose an MID of 5.6.
Calcifying Tendinopathy of the Rotator Cuff: Barbotage vs. Shock Waves: Controlled Clinical Trial Protocol (BOTCH)
Authors: Muñoz-Paz Javier , Piaggio-Muente Fiorella Liz , Acosta-Salvador Sebastián , Gómez-Flores Diego A. , Jiménez-Jiménez Ana Belén , Muñoz-Alcaraz María Nieves , Mayordomo-Riera Fernando Jesús
Published: December 2024

Background: Shoulder pain is a very common health issue among adults, being 8% due to calcifying tendinopathies (CT) of the shoulder. The evolutionary process of this lesion can be classified according to Bianchi Martinoli, depending on the ultrasound appearance. In 50% of cases, with first-line treatments, they resolve spontaneously. However, in the remaining 50%, they become chronic, requiring other lines of treatment, such as shock waves (ESWT) or ultrasound-guided barbotage (US-PICT). Objectives: The objective focuses on comparing the improvement in pain using the visual analgesic scale (VAS) and shoulder joint balance (ROM) in patients with CT based on the treatment received, stratifying according to the characteristics of the injury, with the aim of protocolizing said treatment. Methods: Randomized analytical controlled clinical trial in blocks with two arms according to the Bianchi Martinolli classification (I or II/III) in 56 patients affected by chronic pain by CT. The decision to treat will be made randomly 1:1, based on the treatment assigned to the previous patient. Results will be evaluated in three moments (1, 3, and 6 months). The following variables will be collected: VAS, Lattinen test, ROM (flexion, abduction, external and internal rotation), patient global improvement impression scale (PGI-I), global improvement impression scale (CGI-C). Discussion: The use of ESWT or US-PICT as treatments is a widely used practice in the daily life of this pathology. However, despite knowing that both treatments are useful in chronic CT, there are no known data or protocols by which one therapy is chosen over another, much less the influence that the evolutionary stage can have of the injury in the results obtained.
Transposition of the Great Arteries with Intramural Left Main Coronary Artery—Salient Imaging Findings and Choice of Operative Technique
Authors: Holbert Joshua M. , Gadiraju Manasa , Mehta Samir , Kiaffas Maria , Shah Sanket S. , Bedzra Edo
Published: December 2024

D-transposition of the great arteries (D-TGA) is a common cyanotic critical congenital heart disease. An arterial switch operation (ASO) with/without a ventricular septal defect (VSD) closure is the preferred surgical approach, with an added challenge when an intramural coronary artery (IMC) is present (1), with a reported increased incidence of postoperative complications and mortality (2,3). We present our recent D-TGA with intramural coronary artery (TGA-IMC) experience, focusing on the salient features identified on echocardiography, computed tomography (CT) angiography, and invasive angiograms, as well as variations in ASO surgical techniques for repair. Diagnostic imaging evaluation allowed for identification of the lesion, as well as planning for and undertaking of two different surgical approaches. While the two patients had differing immediate postoperative courses, both were asymptomatic at discharge, with normal biventricular systolic function. Our experience demonstrates that the suspicion for a coronary anomaly in TGA can be raised prenatally and confirmed postnatally with focused trans-thoracic echocardiography and ECG-gated CT angiogram evaluation while also aiding in operative planning. Moreover, suggesting further exploration of the optimal surgical technique for the repair of TGA-IMC.
Digitization of the Lichenotheca Veneta by Vittore Trevisan
Authors: Martellos Stefano , Zardini Maria , Seggi Linda , Conti Matteo , Trabucco Raffaella
Published: December 2024

The Lichenotheca Veneta, published in 1869 by Vittore Trevisan (1818–1897), is one of the most relevant historic collections of exsiccatae of lichens in Italy. It contains a total of 268 specimens from 74 genera, 197 species, and 119 varieties and forms, organized into eight files and four volumes, and was probably conceived as the first of a series, even if no further volumes were published. To our knowledge, it is probably preserved in its complete and original form at the Natural History Museum of Venice only. Given its historical, cultural, and scientific relevance, it has been digitized and the resulting images and metadata have been published in a web portal. The digitization workflow comprised an initial digital imaging phase, followed by the extraction of specimens’ metadata from the specimens’ labels, and by a further digital imaging phase to capture specimens’ relevant details. The mobilization of metadata and images by means of digitization is widely recognized as an effective approach for enhancing the accessibility and usability of natural history collections. At the same time, since several inferences can be made without physically accessing the specimens, which, being biological objects, are intrinsically fragile, digitization contributes to reducing the risk of their deterioration. This contribution details the collection and its features, discussing the digitization process and its results.
Health Outcomes of Construction Workers Building Infrastructure for Mega-Sporting Events: A Systematic Review of the Literature
Authors: Testa Davide J. , Vale João P. , Ioannou Leonidas G. , Dinas Petros C. , Mayor Tiago S. , Onarheim Kristine H. , Babar Zahra R. , Hargreaves Sally , Flouris Andreas D.
Published: December 2024

Background: Migrant construction workers involved in building infrastructure for mega-sporting events face elevated risks of illness and death. However, specific health outcomes for these workers have not been systematically reviewed, limiting opportunities to identify and address their challenges. Methods: This study systematically reviewed health outcomes among migrant construction workers involved in mega-sporting events. Results: 89 eligible studies involving 23,307 workers were identified. Of these, only 11 directly addressed specific health outcomes, including heat stress, occupational fatalities, and sexually transmitted infections. Notably, increased heat exposure during peak construction phases and the proximity of deadlines for mega-sporting events were correlated with elevated rates of occupational fatalities. Other key adverse factors impacting migrant construction workers’ health included an observed correlation between the timing of mega-sporting events and increased occupational fatalities, the involvement of labor recruiters, and shifting health and safety responsibilities among stakeholders (e.g., host states, event organizers, contractors, and recruitment agencies). Positive outcomes were observed when workers voluntarily engaged in non-mandatory safety activities, such as safety training programs and awareness meetings. Conclusions: There is a critical need for longitudinal and comparative studies to comprehensively examine the health of migrant workers throughout all stages of their journey, from pre-migration to return. This review underscores the urgency of prioritizing evidence-based policies that address unique health risks in this population, including mitigation of heat stress and enforcement of occupational safety standards, particularly amid construction spikes preceding mega-sporting events. Recommendations: Future research should prioritize understanding the unique health challenges faced by migrant workers to inform policy making, develop effective interventions, and implement best practices to improve their health and well-being.
Adropin/Tirzepatide Combination Mitigates Cardiac Metabolic Aberrations in a Rat Model of Polycystic Ovarian Syndrome, Implicating the Role of the AKT/GSK3β/NF-κB/NLRP3 Pathway
Authors: Hegab Islam Ibrahim , El-Horany Hemat El-sayed , Abd-Ellatif Rania Nagi , Nasef Nahla Anas , Okasha Asmaa H. , Emam Marwa Nagy , Hassan Shereen , Elseady Walaa S. , Radwan Doaa A. , ElEsawy Rasha Osama , Hafez Yasser Mostafa , Hassan Maha Elsayed , Mansour Nouran Mostafa , Abdelkader Gamaleldien Elsayed , Fouda Mohamed H. , Abd El Maged Amira M. , Abdallah Hanan M.
Published: December 2024

Polycystic ovarian syndrome (PCOS) is a multifaceted metabolic and hormonal disorder in females of reproductive age, frequently associated with cardiac disturbances. This research aimed to explore the protective potential of adropin and/or tirzepatide (Tirze) on cardiometabolic aberrations in the letrozole-induced PCOS model. Female Wistar non-pregnant rats were allotted into five groups: CON; PCOS; PCOS + adropin; PCOS + Tirze; and PCOS + adropin+ Tirze. The serum sex hormones, glucose, and lipid profiles were securitized. Cardiac phosphorylated levels of AKT(pAKT), glycogen synthase kinase-3 beta (pGSK-3β), NOD-like receptor family pyrin domain containing 3 (NLPR3), IL-1β and IL-18 were assayed. The cardiac redox status and endoplasmic reticulum stress (ER) parameters including relative glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) gene expressions were detected. Finally, the immunoreactivity of cardiac NF-κB, Bcl2, and BAX were assessed. Our results displayed that adropin and/or Tirze intervention successfully alleviated the PCOS-provoked cardiometabolic derangements with better results recorded for the combination treatment. The synergistic effect of adropin and Tirze is mostly mediated via activating the cardiac Akt, which dampens the GSK3β/NF-κB/NLRP3 signaling pathway, with a sequel of alleviating oxidative damage, inflammatory response, ER stress, and related apoptosis, making them alluring desirable therapeutic targets in PCOS-associated cardiac complications.
Role Assessment of Water-Soluble Pharmaceutical Form of Phosphatidylcholine on the Catalytic Activity of Cytochrome P450 2C9 and 2D6
Authors: Koroleva Polina , Bulko Tatiana , Kuzikov Alexey , Gilep Andrei , Romashova Yulia , Tichonova Elena , Kostrukova Lyubov , Archakov Alexander , Shumyantseva Victoria
Published: December 2024

This study aimed to investigate whether the water-soluble pharmaceutical form of phosphatidylcholine nanoparticles (wPC) stimulated the catalytic activity of CYP enzymes 2C9 and 2D6. We have shown that electroenzymatic CYP2C9 catalysis to nonsteroidal anti-inflammatory drug naproxen as a substrate was enhanced from 100% to 155% in the presence of wPC in media. Electroenzymatic CYP2D6 activity in the presence of the adrenoceptor-blocking agent bisoprolol as a substrate was elevated significantly from 100% to 144% when wPC was added to potassium phosphate buffer solution. These results indicate the ability of wPC in the form of the phospholipid ultra-small nanoparticles to work as a membrane additive and crowding agent to accelerate the electroenzymatic reactions of cytochrome P450.
Effects of Exercise Training on Cardiac Mitochondrial Functions in Diabetic Heart: A Systematic Review
Authors: Shah Iqbal Ali , Ishaq Shahid , Lee Shin-Da , Wu Bor-Tsang
Published: December 2024

A diabetic heart is characterized by fibrosis, autophagy, oxidative stress, and altered mitochondrial functions. For this review, three databases (PubMed, EMBASE, and Web of Science) were searched for articles written in English from September 2023 to April 2024. Studies that used exercise training for at least 3 weeks and which reported positive, negative, or no effects were included. The CAMARADES checklist was used to assess the quality of the included studies, and ten studies (CAMARADES scores 4–7/10) were included. Nine studies showed that exercise training improved cardiac mitochondrial oxidative phosphorylation by decreasing ROS, increasing electron transport chain activity, and enhancing the production of ATP. Eight studies indicated that exercise training ameliorated mitochondrial biogenesis by increasing the levels of AMPK, PGC-1α, Akt, Irisin, and Sirtuin-III. Moreover, four studies focused on mitochondrial dynamics and concluded that exercise training helped decrease the levels of mitochondrial fission factor and dynamin-related protein- 1. Finally, six studies revealed improvements in mitochondrial physiological characteristics such as size, potential, and permeability. Our findings demonstrate the beneficial effects of exercise training on cardiac mitochondrial function in diabetic hearts. Exercise training improves cardiac mitochondrial physiological characteristics, oxidative phosphorylation, biogenesis, and dynamics.
Developing Allosteric Chaperones for GBA1-Associated Disorders—An Integrated Computational and Experimental Approach
Authors: Montpeyo Marta , Pérez-Carmona Natàlia , Cubero Elena , Delgado Aida , Ruano Ana , Carrillo Jokin , Bellotto Manolo , Martinez-Vicente Marta , Garcia-Collazo Ana Maria
Published: December 2024

Mutations in the GBA1 gene, which encodes the lysosomal enzyme glucocerebrosidase (GCase), are associated with Gaucher disease and increased risk of Parkinson’s disease. This study describes the discovery and characterization of novel allosteric pharmacological chaperones for GCase through an innovative computational approach combined with experimental validation. Utilizing virtual screening and structure-activity relationship optimization, researchers identified several compounds that significantly enhance GCase activity and stability across various cellular models, including patient-derived fibroblasts and neuronal cells harboring GBA1 mutations. Among these, compound 3 emerged as a lead candidate, demonstrating the ability to enhance GCase protein levels and enzymatic activity while effectively reducing the accumulation of toxic substrates in neuronal models. Importantly, pharmacokinetic studies revealed that compound 3 has favorable brain penetration, indicating its potential as a disease-modifying therapy for GBA1-related disorders affecting the central nervous system. This research not only offers a framework for developing allosteric GCase modulators but also unveils promising new therapeutic strategies for managing Gaucher disease and Parkinson’s disease. The ability of compound 3 to cross the blood-brain barrier emphasizes its potential significance in addressing neurological symptoms associated with these conditions.
Changes in the Phenotype and Metabolism of Peritoneal Macrophages in Mucin-2 Knockout Mice and Partial Restoration of Their Functions In Vitro After L-Fucose Treatment
Authors: Arzhanova Elena L. , Makusheva Yulia , Pershina Elena G. , Medvedeva Snezhanna S. , Litvinova Ekaterina A.
Published: December 2024

In the development of inflammatory bowel disease (IBD), peritoneal macrophages contribute to the resident intestinal macrophage pool. Previous studies have demonstrated that oral administration of L-fucose exerts an immunomodulatory effect and repolarizes the peritoneal macrophages in vivo in mice. In this study, we analyzed the phenotype and metabolic profile of the peritoneal macrophages from Muc2−/− mice, as well as the effect of L-fucose on the metabolic and morphological characteristics of these macrophages in vitro. The investigation utilized flow cytometry, quantitative PCR (qPCR), measurement of the intracellular ATP and Ca2+ concentrations, an analysis of mitochondrial respiration and membrane potential, and transmission electron microscopy (TEM) for ultrastructural evaluations. The Muc2−/− mice exhibited lower intracellular ATP and Ca2+ levels in their peritoneal macrophages, a higher percentage of stellate macrophages, and an increased oxygen consumption rate (OCR), combined with a higher percentage of mitochondria displaying an abnormal ultrastructure. Additionally, there was a five-fold increase in condensed mitochondria compared to their level in C57BL/6 mice. The number of CD209+ peritoneal macrophages was reduced three-fold, while the number of M1-like cells increased two-fold in the Muc2−/− mice. L-fucose treatment enhanced ATP production and reduced the expression of the Parp1, Mt-Nd2, and Mt-Nd6 genes, which may suggest a reduction in pro-inflammatory factor production and a shift in the differentiation of peritoneal macrophages towards the M2 phenotype.
Distribution and Maturity of Medial Collagen Fibers in Thoracoabdominal Post-Dissection Aortic Aneurysms: A Comparative Study of Marfan and Non-Marfan Patients
Authors: Doukas Panagiotis , Hruschka Bernhard , Bassett Cathryn , Buhl Eva Miriam , Simon Florian , Saraber Pepijn , Jacobs Michael Johan , Uhl Christian , Schurgers Leon J. , Gombert Alexander
Published: December 2024

Thoracoabdominal aortic aneurysms (TAAAs) are rare but serious conditions characterized by dilation of the aorta characterized by remodeling of the vessel wall, with changes in the elastin and collagen content. Individuals with Marfan syndrome have a genetic predisposition for elastic fiber fragmentation and elastin degradation and are prone to early aneurysm formation and progression. Our objective was to analyze the medial collagen characteristics through histological, polarized light microscopy, and electron microscopy methods across the thoracic and abdominal aorta in twenty-five patients undergoing open surgical repair, including nine with Marfan syndrome. While age at surgery differed significantly between the groups, maximum aortic diameter and aneurysm extent did not. Collagen content increased from thoracic to infrarenal segments in both cohorts, with non-Marfan patients exhibiting higher collagen percentages, notably in the infrarenal aorta (729.3 nm vs. 1068.3 nm, p = 0.02). Both groups predominantly displayed mature collagen fibers, with the suprarenal segment containing the highest proportion of less mature fibers. Electron microscopy revealed comparable collagen fibril diameters across segments irrespective of Marfan status. Our findings underscore non-uniform histological patterns in TAAAs and suggest that ECM remodeling involves mature collagen deposition, albeit with lower collagen content observed in the infrarenal aorta of Marfan patients.
Urinary Proteome Characterization of Stroke-Prone Spontaneously Hypertensive Rats
Authors: Meng Wenshu , Gao Youhe
Published: December 2024

Hypertension is a multifactorial and complex disease influenced by genetic and environmental factors, and it has become one of the most serious public health challenges. This study aimed to investigate the changes in hypertension based on urinary proteome. The stroke-prone spontaneously hypertensive rats (SHRSPs) model was used to examined urinary proteome changes during the development of hypertension. Urine proteome profiling was conducted at months 1, 4, 8, 10, 12, and 14 using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). Given that the progression of hypertension may vary among individuals, each rat was compared before and after hypertension developed to screen for differential proteins. Differential proteins in each rat can be enriched into some important biological processes and pathways associated with hypertension, such as the regulation of systemic arterial blood pressure by renin-angiotensin, renin-angiotensin signaling, response to glucocorticoid and glucocorticoid receptor signaling, calcium transport I, aldosterone adipocyte signaling pathway, apelin adipocyte signaling pathway, and oxidative stress response. The biological processes and pathways enriched at the same time point in the progression of hypertension differed significantly among different rat individuals. This study demonstrated that the changes in hypertension can be reflected in urine proteins. Urinary proteomics has potential in researching the mechanisms underlying hypertension, discovering new drug targets, and developing personalized strategies for antihypertensive treatment.