bluuebunny/crossref_metadata_2025 huggingface dataset:
Population genetic structure of the endangered yellow spotted mountain newt (Neurergus derjugini: Amphibia, Caudata) inferred from mitochondrial DNA sequences
Authors: Salehi Tayebe
Published: January 2019

The yellow spotted mountain newt (Neurergus derjugini) is a critically endangered species restricted to fragmented habitats in highland streams of the middle Zagros Mountain in Iran and Iraq. We examined the species phylogeography by investigating sequences of a mitochondrial fragment of the ND2 gene for 77 individuals from 15 locations throughout the species known distribution. We found relatively high haplotype diversity (0.82 ± 0.025) but low nucleotide diversity (0.0038 ± 0.00022) across all populations. Phylogenetic trees supported monophyly, and the segregation of haplotypes was concordant with the haplotype network. We found a significant correlation between geographical and genetic distances among populations (r = 0.54, P ˂ 0.01), suggesting restricted gene flow. Molecular dating suggested that haplogroups diverged during the early or middle Pleistocene. Bayesian skyline plot provided evidence for an expansion of populations during the Pleistocene-Holocene transition period. Taken together, isolation by distance due to low dispersal capability, habitat fragmentation, and historical factors have shaped the current population structure of N. derjugini.
Antioxidant Response to Salinity in Salt-Tolerant and Salt-Susceptible Cultivars of Date Palm
Authors: Al Kharusi Latifa , Al Yahyai Rashid , Yaish Mahmoud W.
Published: January 2019

The salinity tolerance mechanism in date palm through antioxidation has not been completely deciphered to date. Therefore, this study aimed to investigate the role of various antioxidants in salinity tolerance. Two date palm cultivars, contrasting in salinity tolerance, were used as model plants in a comparative study designed to detect changes in growth, Na+ and K+ uptake, reactive oxygen species (ROS), and antioxidant accumulations, when plants were exposed to salt stress. The results showed that salinity treatment had a more substantial negative effect on the growth and photosynthetic pigmentation of the susceptible ‘Zabad’ cultivar than on the tolerant ‘Umsila’ cultivar, probably due to the ability of ‘Umsila’ to accumulate less Na+ and more K+, to maintain a normal concentration of ROS and to produce more non-enzymatic antioxidants, including glutathione, phenolic compounds, flavonoids, and proline. Under salinity, ‘Umsila’ could also activate more superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) than ‘Zabad’. These results suggest that the tolerance of ‘Umsila’ is partially due to the balanced Na+ and K+ uptake and to the relatively high concentration of ROS-scavenging metabolites. Together, these results indicate that the antioxidant mechanism is crucial for salinity tolerance in date palms. However, other mechanisms may also be involved in this trait.
Acknowledgement to Reviewers of Antibodies in 2018
Authors:   Antibodies Editorial Office
Published: January 2019

Peer review is an essential part of the publication process, ensuring that Antibodies maintains high quality standards for its published papers. [...]
Peroxiredoxins in Cancer and Response to Radiation Therapies
Authors: Forshaw Tom E. , Holmila Reetta , Nelson Kimberly J. , Lewis Joshua E. , Kemp Melissa L. , Tsang Allen W. , Poole Leslie B. , Lowther W. Todd , Furdui Cristina M.
Published: January 2019

Peroxiredoxins have a long-established cellular function as regulators of redox metabolism by catalyzing the reduction of peroxides (e.g., H2O2, lipid peroxides) with high catalytic efficiency. This activity is also critical to the initiation and relay of both phosphorylation and redox signaling in a broad range of pathophysiological contexts. Under normal physiological conditions, peroxiredoxins protect normal cells from oxidative damage that could promote oncogenesis (e.g., environmental stressors). In cancer, higher expression level of peroxiredoxins has been associated with both tumor growth and resistance to radiation therapies. However, this relationship between the expression of peroxiredoxins and the response to radiation is not evident from an analysis of data in The Cancer Genome Atlas (TCGA) or NCI60 panel of cancer cell lines. The focus of this review is to summarize the current experimental knowledge implicating this class of proteins in cancer, and to provide a perspective on the value of targeting peroxiredoxins in the management of cancer. Potential biases in the analysis of the TCGA data with respect to radiation resistance are also highlighted.
Quantification of Unknown Nanoscale Biomolecules Using the Average-Weight-Difference Method
Authors: Lee Kyungwoo , Choi Yonghyun , Kim Kyobum , Koo Hyung-Jun , Choi Jonghoon
Published: January 2019

In order to quantify the amount of drug molecules in drug delivery systems, analytical techniques such as high-performance liquid chromatography are used due to their accuracy and reliability. However, the instruments required for such techniques are expensive and not available in all laboratories. Therefore, in this study, we introduce a method that can be a relatively inexpensive and easy to perform drug analysis in almost any laboratory set-up. We have devised the “average-weight-difference method” within the limits of existing spectral analyses. By employing this method, we quantitatively analyzed the amount of isoniazid or doxorubicin molecules loaded onto β-glucan nanoparticles. This proved to be a relatively simple and reliable method and can be used to estimate the amount of nanoscale biomolecules before their analysis through expensive equipment in an environment where the instruments are not readily available.
A Virtual Network Resource Allocation Framework Based on SR-IOV
Authors: Ye Zhiyong , Zhong Yuanchang , Wei Yingying
Published: January 2019

The workload of a data center has the characteristics of complexity and requirement variability. However, in reality, the attributes of network workloads are rarely used by resource schedulers. Failure to dynamically schedule network resources according to workload changes inevitably leads to the inability to achieve optimal throughput and performance when allocating network resources. Therefore, there is an urgent need to design a scheduling framework that can be workload-aware and allocate network resources on demand based on network I/O virtualization. However, in the current mainstream I/O virtualization methods, there is no way to provide workload-aware functions while meeting the performance requirements of virtual machines (VMs). Therefore, we propose a method that can dynamically sense the VM workload to allocate network resources on demand, and can ensure the scalability of the VM while improving the performance of the system. We combine the advantages of I/O para-virtualization and SR-IOV technology, and use a limited number of virtual functions (VFs) to ensure the performance of network-intensive VMs, thereby improving the overall network performance of the system. For non-network-intensive VMs, the scalability of the system is guaranteed by using para-virtualized Network Interface Cards (NICs) which are not limited in number. Furthermore, to be able to allocate the corresponding bandwidth according to the VM’s network workload, we hierarchically divide the VF’s network bandwidth, and dynamically switch between VF and para-virtualized NICs through the active backup strategy of Bonding Drive and ACPI Hotplug technology to ensure the dynamic allocation of VF. Experiments show that the allocation framework can effectively improve system network performance, in which the average request delay can be reduced by more than 26%, and the system bandwidth throughput rate can be improved by about 5%.
Evaluation of Some Rhodes Grass (Chloris gayana) Genotypes for Their Salt Tolerance, Biomass Yield and Nutrient Composition
Authors: Daba Ashenafi Worku , Qureshi Asad Sarwar , Nisaren Bethel Nekir
Published: January 2019

The livestock productivity in Ethiopia is seriously constrained by the shortage of fodder due to increasing soil salinization. Therefore, restoration of salt-affected lands into productive soils through salt-tolerant forages and improved irrigation and crop management practices is crucial for enhancing the productivity of the livestock sector in Ethiopia. In this three-year study, pot trials were conducted to evaluate the impact of five different soil salinity levels (i.e., 0, 5, 10, 15, and 20 dS m−1) on plant growth, biomass production, and nutrient quality attributes of three Rhodes grass (Chloris gayana) genotypes (ILRI-6633, ILRI-7384, CV-massaba). Increasing soil salinity negatively affected germination percentage (GP) and mean germination time (MGT) of all genotypes. For all salinity levels, the highest GP was observed for ILRI-6633 and the lowest for CV-massaba. Plant height and chlorophyll content for ILRI-6633 was higher than the other two genotypes. The crude protein (CP) content was higher in low dry matter-producing genotype (ILRI-7384). The performance of ILRI-6633 at all salinity levels was superior to the other two genotypes. CV-massaba genotype performed better under low to medium soil salinity conditions. Therefore, ILRI-6633 and CV-massaba genotypes have excellent potential to increase forage production in salt-affected areas of Ethiopia.
A Systematic Review of Open Source Clinical Software on GitHub for Improving Software Reuse in Smart Healthcare
Authors: Shen Zhengru , Spruit Marco
Published: January 2019

The plethora of open source clinical software offers great reuse opportunities for developers to build clinical tools at lower cost and at a faster pace. However, the lack of research on open source clinical software poses a challenge for software reuse in clinical software development. This paper aims to help clinical developers better understand open source clinical software by conducting a thorough investigation of open source clinical software hosted on GitHub. We first developed a data pipeline that automatically collected and preprocessed GitHub data. Then, a deep analysis with several methods, such as statistical analysis, hypothesis testing, and topic modeling, was conducted to reveal the overall status and various characteristics of open source clinical software. There were 14,971 clinical-related GitHub repositories created during the last 10 years, with an average annual growth rate of 55%. Among them, 12,919 are open source clinical software. Our analysis unveiled a number of interesting findings: Popular open source clinical software in terms of the number of stars, most productive countries that contribute to the community, important factors that make an open source clinical software popular, and 10 main groups of open source clinical software. The results can assist both researchers and practitioners, especially newcomers, in understanding open source clinical software.
Improving Surface Roughness of Additively Manufactured Parts Using a Photopolymerization Model and Multi-Objective Particle Swarm Optimization
Authors: Kim Namjung , Bhalerao Ishan , Han Daehoon , Yang Chen , Lee Howon
Published: January 2019

Although additive manufacturing (AM) offers great potential to revolutionize modern manufacturing, its layer-by-layer process results in a staircase-like rough surface profile of the printed part, which degrades dimensional accuracy and often leads to a significant reduction in mechanical performance. In this paper, we present a systematic approach to improve the surface profile of AM parts using a computational model and a multi-objective optimization technique. A photopolymerization model for a micro 3D printing process, projection micro-stereolithography (PμSL), is implemented by using a commercial finite element solver (COMSOL Multiphysics software). First, the effect of various process parameters on the surface roughness of the printed part is analyzed using Taguchi’s method. Second, a metaheuristic optimization algorithm, called multi-objective particle swarm optimization, is employed to suggest the optimal PμSL process parameters (photo-initiator and photo-absorber concentrations, layer thickness, and curing time) that minimize two objectives; printing time and surface roughness. The result shows that the proposed optimization framework increases 18% of surface quality of the angled strut even at the fastest printing speed, and also reduces 50% of printing time while keeping the surface quality equal for the vertical strut, compared to the samples produced with non-optimized parameters. The systematic approach developed in this study significantly increase the efficiency of optimizing the printing parameters compared to the heuristic approach. It also helps to achieve 3D printed parts with high surface quality in various printing angles while minimizing printing time.
Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar
Authors: Luo Jie , Xia Wenxiu , Cao Pei , Xiao Zheng’ang , Zhang Yan , Liu Meifeng , Zhan Chang , Wang Nian
Published: January 2019

Plants have evolved a sophisticated system to respond to various stresses. Fungal attack or infection is one of the most important biotic stresses for most plants. During the defense response to fungal infection, the plant hormones jasmonic acid (JA) and salicylic acid (SA) play critical roles. Here, gene expression data on JA/SA treatments and Melampsora larici-populina (MLP) infection were generated. Integrated transcriptome analyses of these data were performed, and 943 genes in total were identified as common responsive genes (CRG). Gene ontology (GO) term analysis revealed that the genes from CRG are generally involved in the processes of stress responses, metabolism, and growth and development. The further cluster analysis of the CRG identified a set of core genes that are involved in the JA/SA-mediated response to fungal defense with distinct gene expression profiles upon JA/SA treatment, which highlighted the different effects of these two hormones on plant fungal defenses. The modifications of several pathways relative to metabolism, biotic stress, and plant hormone signal pathways suggest the possible roles of JA/SA on the regulation of growth and defense responses. Co-expression modules (CMs) were also constructed using the poplar expression data on JA, SA, M. larici-populina, Septoria musiva, and Marssonina brunnea treatment or infection. A total of 23 CMs were constructed, and different CMs clearly exhibited distinct biological functions, which conformably regulated the concerted processes in response to fungal defense. Furthermore, the GO term analysis of different CMs confirmed the roles of JA and SA in regulating growth and defense responses, and their expression profiles suggested that the growth ability was reduced when poplar deployed defense responses. Several transcription factors (TFs) among the CRG in the co-expression network were proposed as hub genes in regulating these processes. According to this study, our data finely uncovered the possible roles of JA/SA in regulating the balance between growth and defense responses by integrating multiple hormone signaling pathways. We were also able to provide more knowledge on how the plant hormones JA/SA are involved in the regulation of the balance between growth and plant defense.
Toxicity Mechanism of Low Doses of NaGdF4:Yb3+,Er3+ Upconverting Nanoparticles in Activated Macrophage Cell Lines
Authors: Wysokińska Edyta , Cichos Jakub , Kowalczyk Agnieszka , Karbowiak Mirosław , Strządała Leon , Bednarkiewicz Artur , Kałas Wojciech
Published: January 2019

Gadolinium-doped nanoparticles (NPs) are regarded as promising luminescent probes. In this report, we studied details of toxicity mechanism of low doses of NaGdF4-based fluorescent nanoparticles in activated RAW264.7, J774A.1 macrophages. These cell lines were specifically sensitive to the treatment with nanoparticles. Using nanoparticles of three different sizes, but with a uniform zeta potential (about −11 mV), we observed rapid uptake of NPs by the cells, resulting in the increased lysosomal compartment and subsequent superoxide induction along with a decrease in mitochondrial potential, indicating the impairment of mitochondrial homeostasis. At the molecular level, this led to upregulation of proapoptotic Bax and downregulation of anti-apoptotic Bcl-2, which triggered the apoptosis with phosphatidylserine externalization, caspase-3 activation and DNA fragmentation. We provide a time frame of the toxicity process by presenting data from different time points. These effects were present regardless of the size of nanoparticles. Moreover, despite the stability of NaGdF4 nanoparticles at low pH, we identified cell acidification as an essential prerequisite of cytotoxic reaction using acidification inhibitors (NH4Cl or Bafilomycin A1). Therefore, approaching the evaluation of the biocompatibility of such materials, one should keep in mind that toxicity could be revealed only in specific cells. On the other hand, designing gadolinium-doped NPs with increased resistance to harsh conditions of activated macrophage phagolysosomes should prevent NP decomposition, concurrent gadolinium release, and thus the elimination of its toxicity.
A Better Maintenance Strategy, a More Sustainable Hanok: Towards Korean Traditional Public Facilities
Authors: Jeong Jeong-Hyuk , Cheon Deuk-Youm , Han Seung-Hoon
Published: January 2019

Currently, user demands for Hanok, the Korean traditional building type, are increasing in Korea, and their use as residences and accommodations are especially booming, while public facilities are rarely built in the style of Hanok these days. One of the most critical reasons for the issue is that Hanok lack usability and are difficult to maintain as a public facilities. Therefore, it is improvement of the usability of Hanok is needed for them to be accepted as public buildings and to set up the maintenance strategy for the style of the wooden structure. This research has defined public buildings in Hanok and classified them into three types according to structural standards. Then, this study analyzed the characteristics of each type, and suggested a checklist for Hanok usability, employing it to analyze the most recent exemplar Hanoks built as results in the governmental research project, monitored them in aspects of the facility management focused on the wooden structure and suggested strategies for sustainability by deriving eight factors in usability and another eight major types of defects to review the current status of the maintenance for the wooden structure. Finally, this study proposed the main direction for Hanok maintenance to establish its strategies for sustainability.
Boosting the Characterization of Heterogeneous Catalysts for H2O2 Direct Synthesis by Infrared Spectroscopy
Authors: Manzoli Maela
Published: January 2019

Infrared (IR) spectroscopy is among the most powerful spectroscopic techniques available for the morphological and physico-chemical characterization of catalytic systems, since it provides information on (i) the surface sites at an atomic level, (ii) the nature and structure of the surface or adsorbed species, as well as (iii) the strength of the chemical bonds and (iv) the reaction mechanism. In this review, an overview of the main contributions that have been determined, starting from IR absorption spectroscopy studies of catalytic systems for H2O2 direct synthesis, is given. Which kind of information can be extracted from IR data? IR spectroscopy detects the vibrational transitions induced in a material by interaction with an electromagnetic field in the IR range. To be IR active, a change in the dipole moment of the species must occur, according to well-defined selection rules. The discussion will be focused on the advancing research in the use of probe molecules to identify (and possibly, quantify) specific catalytic sites. The experiments that will be presented and discussed have been carried out mainly in the mid-IR frequency range, between approximately 700 and 4000 cm−1, in which most of the molecular vibrations absorb light. Some challenging possibilities of utilizing IR spectroscopy for future characterization have also been envisaged.
Photocatalytic Hydrogen Production Under Near-UV Using Pd-Doped Mesoporous TiO2 and Ethanol as Organic Scavenger
Authors: Rusinque Bianca , Escobedo Salvador , Lasa Hugo de
Published: January 2019

Photocatalysis can be used advantageously for hydrogen production using a light source (near-UV light), a noble metal-doped semiconductor and an organic scavenger (2.0 v/v% ethanol). With this end, palladium was doped on TiO2 photocatalysts at different metal loadings (0.25 to 5.00 wt%). Photocatalysts were synthetized using a sol-gel method enhancing morphological properties with a soft template precursor. Experiments were carried out in the Photo-CREC Water II reactor system developed at CREC-UWO (Chemical Reactor Engineering Centre- The University of Western Ontario) Canada. This novel unit offers hydrogen storage and symmetrical irradiation allowing precise irradiation measurements for macroscopic energy balances. Hydrogen production rates followed in all cases a zero-order reaction, with quantum yields as high as 30.8%.
Direct Catalytic Conversion of CO2 to Cyclic Organic Carbonates under Mild Reaction Conditions by Metal—Organic Frameworks
Authors: Huh Seong
Published: January 2019

The reduction of the representative greenhouse gas, carbon dioxide (CO2), is significantly an important theme for the current research in the modern chemical world. For the last two decades, the development of new metal-organic framework (MOF) systems with highly selective capture of CO2, in the presence of other competing gaseous molecules, has flourished to capture or separate CO2 for environmental protection. Nonetheless, the ultimate resolution to lessen the atmospheric CO2 concentration may be in the chemical or electrochemical conversion of CO2 to other compounds. In this context, the catalytic cycloaddition reaction of CO2 into organic epoxides to produce cyclic carbonates is a more attractive method. MOFs are being proven as efficient heterogeneous catalytic systems for this important reaction. In this review, we collected very recent progress in MOF-based catalytic systems, fully operable under very mild reaction conditions (room temperature and 1 atm CO2).
Mechanochemical Preparation of Novel Polysaccharide-Supported Nb2O5 Catalysts
Authors: Rincon Esther , Garcia Araceli , Romero Antonio A. , Serrano Luis , Luque Rafael , Balu Alina M.
Published: January 2019

Polysaccharides extracted from natural sources can be used as starting material for the preparation of nanoparticle supported composites. A novel family of bio-nanocomposites was mechanochemically synthesized by using niobium oxide and enzymatically produced polysaccharides. The structural, textural and surface properties of nanomaterials, were determined by X-Ray diffraction (XRD), nitrogen adsorption-desorption (N₂ porosimetry), pulse chromatography, infrared spectroscopy (ATR-IR) and dynamic light scattering (DLS). Selective oxidation of isoeugenol to vanillin was carried out to demonstrate the catalytic activity of the Nb-polysaccharides nanocomposites. Interestingly, most of our material showed high conversion of isoeugenol (60–70%) with selectivity to vanillin over 40%. The optimum conversion and selectivity were achieved with a reaction time between 8 and 24 h.
Progress in Modeling of Silica-Based Membranes and Membrane Reactors for Hydrogen Production and Purification
Authors: Ghasemzadeh Kamran , Basile Angelo , Iulianelli Adolfo
Published: January 2019

Hydrogen is seen as the new energy carrier for sustainable energy systems of the future. Meanwhile, proton exchange membrane fuel cell (PEMFC) stacks are considered the most promising alternative to the internal combustion engines for a number of transportation applications. Nevertheless, PEMFCs need high-grade hydrogen, which is difficultly stored and transported. To solve these issues, generating hydrogen using membrane reactor (MR) systems has gained great attention. In recent years, the role of silica membranes and MRs for hydrogen production and separation attracted particular interest, and a consistent literature is addressed in this field. Although most of the scientific publications focus on silica MRs from an experimental point of view, this review describes the progress done in the last two decades in terms of the theoretical approach to simulate silica MR performances in the field of hydrogen generation. Furthermore, future trends and current challenges about silica membrane and MR applications are also discussed.
Effect of Surface Topography and Structural Parameters on the Lubrication Performance of a Water-Lubricated Bearing: Theoretical and Experimental Study
Authors: Xie Zhongliang , Rao Zhushi , Liu Huanling
Published: January 2019

This study explored the influence of the surface topography of a bushing on the lubrication performance of a water-lubricated bearing. Bushing deformations were considered in the mathematical model. Theoretical calculations and experiments were performed. The test data corresponded well with the simulation. The main stiffness and cross stiffness coefficients were measured and compared with the theoretical values, and the empirical formula of friction coefficient was fitted based on the test data.
Embedded Microcontroller with a CCD Camera as a Digital Lighting Control System
Authors: Adam George K. , Kontaxis Panagiotis A. , Doulos Lambros T. , Madias Evangelos-Nikolaos D. , Bouroussis Constantinos A. , Topalis Frangiskos V.
Published: January 2019

Although with the advent of the LEDs the energy consumption in buildings can be reduced by 50%, there exists a potential for energy savings due to lighting controls. Moreover, lighting controls can ensure that the near zero energy requirements by EU can be achieved for near zero energy buildings (nZEBs). For this reason, more sophisticated lighting controls must be proposed in order to take full advantage of LEDs and their flexibility concerning dimming. This paper proposes the architecture of an embedded computer camera controller for monitoring and management of image data, which is applied in various control cases, and particularly in digitally controlled lighting devices. The proposed system deals with real-time monitoring and management of a GigE camera input. An in-house developed algorithm using MATLAB enables the identification of areas in luminance values. The embedded microcontroller is part of a complete lighting control system with an imaging sensor in order to measure and control the illumination of several working areas of a room. The power consumption of the proposed lighting system was measured and was compared with the power consumption of a typical photosensor. The functional performance and operation of the proposed camera control system architecture was evaluated based upon a BeagleBone Black microcontroller board.
Design, Simulation and Hardware Implementation of Shunt Hybrid Compensator Using Synchronous Rotating Reference Frame (SRRF)-Based Control Technique
Authors: Balasubramanian R. , Parkavikathirvelu K. , Sankaran R. , Amirtharajan Rengarajan
Published: January 2019

This paper deals with the design, simulation, and implementation of shunt hybrid compensator to maintain the power quality in three-phase distribution networks feeding different types balanced and unbalanced nonlinear loads. The configuration of the compensator consists of a selective harmonic elimination passive filter, a series-connected conventional six-pulse IGBT inverter, acting as the active filter terminated with a DC link capacitor. The theory and modelling of the compensator based on current harmonic components at the load end and their decomposition in d-q axis frame of reference are utilized in the reference current generation algorithm. Accordingly, the source current waveform is made to follow the reference current waveform using a high-frequency, carrier-based controller. Further, this inner current control loop is supported by a slower outer voltage control loop for sustaining desirable DC link voltage. Performance of the compensator is evaluated through MATLAB simulation covering different types of loads and reduction of harmonic currents and THD at the supply side along with excellent regulation of DC link voltage are confirmed. The performance of a hybrid compensator designed and fabricated using the above principles is evaluated and corroborated with the simulation results.
Comparative Study of Indoor Propagation Model Below and Above 6 GHz for 5G Wireless Networks
Authors: Al-Samman Ahmed Mohammed , Abd. Rahman Tharek , Al-Hadhrami Tawfik , Daho Abdusalama , Hindia MHD Nour , Azmi Marwan Hadri , Dimyati Kaharudin , Alazab Mamoun
Published: January 2019

It has been widely speculated that the performance of the next generation based wireless network should meet a transmission speed on the order of 1000 times more than the current cellular communication systems. The frequency bands above 6 GHz have received significant attention lately as a prospective band for next generation 5G systems. The propagation characteristics for 5G networks need to be fully understood for the 5G system design. This paper presents the channel propagation characteristics for a 5G system in line of sight (LOS) and non-LOS (NLOS) scenarios. The diffraction loss (DL) and frequency drop (FD) are investigated based on collected measurement data. Indoor measurement results obtained using a high-resolution channel sounder equipped with directional horn antennas at 3.5 GHz and 28 GHz as a comparative study of the two bands below and above 6 GHz. The parameters for path loss using different path loss models of single and multi-frequencies have been estimated. The excess delay, root mean square (RMS) delay spread and the power delay profile of received paths are analyzed. The results of the path loss models show that the path loss exponent (PLE) in this indoor environment is less than the free space path loss exponent for LOS scenario at both frequencies. Moreover, the PLE is not frequency dependent. The 3GPP path loss models for single and multi-frequency in LOS scenarios have good performance in terms of PLE that is as reliable as the physically-based models. Based on the proposed models, the diffraction loss at 28 GHz is approximately twice the diffraction loss at 3.5 GHz. The findings of the power delay profile and RMS delay spread indicate that these parameters are comparable for frequency bands below and above 6 GHz.
Effects of Seawater on Carotenoid Production and Lipid Content of Engineered Saccharomyces cerevisiae
Authors: Guo Yuqi , Xie Shangxian , Yuan Joshua S. , Kao Katy C.
Published: January 2019

The use of seawater in fermentation can potentially reduce the freshwater burden in the bio-based production of chemicals and fuels. We previously developed a Saccharomyces cerevisiae carotenoids hyperproducer SM14 capable of accumulating 18 mg g−1 DCW (DCW: dry cell weight) of β-carotene in rich media (YPD). In this work, the impacts of seawater on the carotenoid production of SM14 were investigated. When using nutrient-reduced media (0.1× YNB) in freshwater the β-carotene production of SM14 was 6.51 ± 0.37 mg g−1 DCW; however in synthetic seawater, the production was increased to 8.67 ± 0.62 mg g−1 DCW. We found that this improvement was partially due to the NaCl present in the synthetic seawater, since supplementation of 0.5 M NaCl in freshwater increased β-carotene production to 11.85 ± 0.77 mg g−1 DCW. The combination of synthetic seawater with higher carbon-to-nitrogen ratio (C:N = 50) further improved the β-carotene production to 10.44 ± 0.35 mg g−1 DCW. We further showed that the carotenoid production improvement in these conditions is related with lipid content and composition. These results demonstrated the benefit of using seawater to improve the production of carotenoids in S. cerevisiae, and have the potential to expand the utilization of seawater.
Identification of a Novel Nucleobase-Ascorbate Transporter Family Member in Fish and Amphibians
Authors: Oliveira Diogo , M. Machado André , Cardoso Tiago , Lopes-Marques Mónica , C. Castro L. Filipe , Ruivo Raquel
Published: January 2019

Nucleobase-Ascorbate Transporter (NAT) family includes ascorbic acid, nucleobases, and uric acid transporters: With broad evolutionary distribution. In vertebrates, four members have been previously recognized, the ascorbate transporters Slc23a1 and Slc3a2, the nucleobase transporter Slc23a4 and an orphan transporter Slc23a3. Using phylogenetic and synteny analysis, we identify a fifth member of the vertebrate slc23 complement (slc23a5), present in neopterygians (gars and teleosts) and amphibians, and clarify the evolutionary relationships between the novel gene and known slc23 genes. Further comparative analysis puts forward uric acid as the preferred substrate for Slc23a5. Gene expression quantification, using available transcriptomic data, suggests kidney and testis as major expression sites in Xenopus tropicalis (western clawed frog) and Danio rerio (zebrafish). Additional expression in brain was detected in D. rerio, while in the Neoteleostei Oryzias latipes (medaka) slc23a5 expression is restricted to the brain. The biological relevance of the retention of an extra transporter in fish and amphibians is discussed.
Safety of Yam-Derived (Dioscorea rotundata) Foodstuffs—Chips, Flakes and Flour: Effect of Processing and Post-Processing Conditions
Authors: Omohimi Celestina , Piccirillo Clara , Ferraro Vincenza , Roriz Mariana C. , Omemu Mobolaji A. , Santos Sandra M. Dias , Da Ressurreição Sandrine , Abayomi Louise , Adebowale Abdulraqaz , Vasconcelos Marta W. , Obadina Oluwasegun , Sanni Lateef , Pintado Maria M. E.
Published: January 2019

The production of yam-derived (Dioscorea rotundata) foodstuffs is mainly performed by small and medium scale processors that employ old traditional methods. This can lead to differences in quality from processor to processor, and from location to location, with consequent safety concerns. As such, the effects of processing and post-processing phases (i.e., storage, transport, etc.) on the safety of some yam-derived foodstuffs—namely chips, flakes, and flour—has been evaluated, with a focus on bacterial and fungal contamination, aflatoxins, pesticides, and heavy metals (Pb, Ni, Cd and Hg). Yams harvested and processed in Nigeria were screened, being that the country is the largest producer of the tuber, with 70–75% of the world production. Results highlighted no presence of pesticides, however, many samples showed high levels of bacterial and fungal contamination, together with heavy metal concentrations above the recommended safety levels. No trend was observed between the items considered; it was noticed, however, that samples purchased from the markets showed higher contamination levels than those freshly produced, especially regarding bacterial and aflatoxins presence. The processing stage was identified as the most critical, especially drying. Nonetheless, post-processing steps such as storage and handling at the point of sale also contributed for chemical contamination, such as aflatoxin and heavy metals. The results suggested that both the processing and post-processing phases have an impact on the safety of yam chips, flakes, and flour.
Halo Concentrations and the Fundamental Plane of Galaxy Clusters
Authors: Fujita Yutaka , Donahue Megan , Ettori Stefano , Umetsu Keiichi , Rasia Elena , Meneghetti Massimo , Medezinski Elinor , Okabe Nobuhiro , Postman Marc
Published: January 2019

According to the standard cold dark matter (CDM) cosmology, the structure of dark halos including those of galaxy clusters reflects their mass accretion history. Older clusters tend to be more concentrated than younger clusters. Their structure, represented by the characteristic radius r s and mass M s of the Navarro–Frenk–White (NFW) density profile, is related to their formation time. In this study, we showed that r s , M s , and the X-ray temperature of the intracluster medium (ICM), T X , form a thin plane in the space of ( log r s , log M s , log T X ) . This tight correlation indicates that the ICM temperature is also determined by the formation time of individual clusters. Numerical simulations showed that clusters move along the fundamental plane as they evolve. The plane and the cluster evolution within the plane could be explained by a similarity solution of structure formation of the universe. The angle of the plane shows that clusters have not achieved “virial equilibrium” in the sense that mass/size growth and pressure at the boundaries cannot be ignored. The distribution of clusters on the plane was related to the intrinsic scatter in the halo concentration–mass relation, which originated from the variety of cluster ages. The well-known mass–temperature relation of clusters ( M Δ ∝ T X 3 / 2 ) can be explained by the fundamental plane and the mass dependence of the halo concentration without the assumption of virial equilibrium. The fundamental plane could also be used for calibration of cluster masses.
Monitoring the Italian Home Palliative Care Services
Authors: Scaccabarozzi Gianlorenzo , Lovaglio Pietro Giorgio , Limonta Fabrizio , Peruselli Carlo , Bellentani Mariadonata , Crippa Matteo
Published: January 2019

Background: In Italy, there currently is a lack of reliable and consistent data on home palliative care provided to people near death. Objectives: Monitoring the activities of the Italian Home Palliative Care Services, according to the 2014 national data collection program entitled “Observatory of Best Practices in Palliative Care” and providing process/outcome measures on a subsample (Best Practice Panel), on regulatory standards and on complete/reliable activity data. Design: A data collection web portal using two voluntary internet-based questionnaires in order to retrospectively identify the main care activity data provided within the year 2013 by Home care units. In the Best Practice Panel and International best practices, eligibility and quality measures refer to the national standards of the NL 38/2010. Setting/Subject: Home Palliative Care Services (HPCSs) that provided care from January to December 2013. Results: 118 Home care units were monitored, globally accounting for 40,955 assisted patients within the year 2013 (38,384 cancer patients); 56 (47.5% of 118) were admitted in the Best Practice Panel. Non-cancer (5%) and pediatric (0.4%) patients represented negligible percentages of frail care patients, and a majority of patients died at home (respectively nearly 75% and 80% of cancer and non-cancer patients). Conclusion: The study demonstrated the feasibility of the collection of certified data from Home care services through a web-based system. Only 80% of the facilities met the requirements provided by the Italian NL 38/2010. Moreover, the extension of the palliative care services provided to frail non-cancer and pediatric patients, affected by complex and advanced chronic conditions, is still inadequate in Italy.
Polymorphisms in ERCC5 rs17655 and ERCC1 rs735482 Genes Associated with the Survival of Male Patients with Postoperative Oral Squamous Cell Carcinoma Treated with Adjuvant Concurrent Chemoradiotherapy
Authors: Senghore Thomas , Chien Huei-Tzu , Wang Wen-Chang , Chen You-Xin , Young Chi-Kuang , Huang Shiang-Fu , Yeh Chih-Ching
Published: January 2019

The nucleotide excision repair (NER) pathway plays a major role in the repair of DNA damaged by exogenous agents, such as chemotherapeutic and radiotherapeutic agents. Thus, we investigated the association between key potentially functional single nucleotide polymorphisms (SNPs) in the NER pathway and clinical outcomes in oral squamous cell carcinoma (OSCC) patients treated with concurrent chemoradiotherapy (CCRT). Thirteen SNPs in five key NER genes were genotyped in 319 male OSCC patients using iPLEX MassARRAY. Cox proportional hazards models and Kaplan–Meier survival curves were used to estimate the risk of death or recurrence. Carriers of the XPC rs2228000 TT genotype showed a borderline significant increased risk of poor overall survival under the recessive model (hazard ratio (HR) = 1.81, 95% confidence interval (CI) = 0.99–3.29). The CC genotypes of ERCC5 rs17655 (HR = 1.54, 95% CI = 1.03–2.29) and ERCC1 rs735482 (HR = 1.65, 95% CI = 1.06–2.58) were associated with an increased risk of worse disease-free survival under the recessive model. In addition, participants carrying both the CC genotypes of ERCC5 rs17655 and ERCC1 rs735482 exhibited an enhanced susceptibility for recurrence (HR = 2.60, 95% CI = 1.11–6.09). However, no statistically significant interaction was observed between them. Our findings reveal that the ERCC5 rs17655 CC and ERCC1 rs735482 CC genotypes were associated with an increased risk of recurrence in male patients with OSCC treated with CCRT. Therefore, CCRT may not be beneficial, and alternative treatments are required for such patients.
Safety of Permanent Pacemaker Implantation: A Prospective Study
Authors: Carrión-Camacho Mª Reyes , Marín-León Ignacio , Molina-Doñoro José Manuel , González-López José Rafael
Published: January 2019

Although pacemaker implantation is considered to be low risk, it is not exempt from complications and technical failures during the procedure, both in the short and long term, and the complications that such patients may present remain unknown. The aim has been to analyze the complication rates associated with permanent pacing and to identify if these differ between patients with or without previous antithrombotic therapy. We used a prospective, single center, observational study of 310 adult patients with indications of permanent pacing. They were hospitalized from 1 January to 31 December 2014 and followed up for 6 months after the pacemaker implant. The participants were distributed into two groups according to the antithrombotic therapy prior to the implant. The most frequent major complications were pneumothorax (3.87%) and lead dislodgement (8.39%), while superficial phlebitis (12.90%) and uncomplicated hematomas (22.58%) were presented as the most recurrent minor complications. Hematomas were the most frequent minor complication in the antithrombotic therapy cohort, and shoulder pain was reported as the most recurrent minor complication in the non-exposed group. Finding out about complications in pacemaker implants enables a complete view of the process, and hence the prioritization of actions aimed at improving safety and reducing associated risks.
Epilepsy in Children: From Diagnosis to Treatment with Focus on Emergency
Authors: Minardi Carmelo , Minacapelli Roberta , Valastro Pietro , Vasile Francesco , Pitino Sofia , Pavone Piero , Astuto Marinella , Murabito Paolo
Published: January 2019

Seizures are defined as a transient occurrence of signs and symptoms due to the abnormal, excessive, or synchronous neuronal activity in the brain characterized by abrupt and involuntary skeletal muscle activity. An early diagnosis, treatment, and specific medical support must be performed to prevent Status Epilepticus (SE). Seizure onset, especially in the child population, is related to specific risk factors like positive family history, fever, infections, neurological comorbidity, premature birth, mother’s alcohol abuse, and smoking in pregnancy. Early death risk in children without neurological comorbidity is similar to the general population. Diagnosis is generally based on the identification of continuous or recurrent seizures but Electroencephalogram (EEG) evaluation could be useful if SE condition is suspected. The main goal of therapy is to counteract the pathological mechanism which occurs in SE before neural cells are irreversibly damaged. According to the latest International Guidelines and Recommendations of seizure related diseases, a schematic and multi-stage pharmacological and diagnostic approach is proposed especially in the management of SE and its related causes in children. First measures should focus on early and appropriate drugs administration at adequate dosage, airway management, monitoring vital signs, Pediatric Intensive Care Unit (PICU) admission, and management of parent anxiety.
Biomaterials, Current Strategies, and Novel Nano-Technological Approaches for Periodontal Regeneration
Authors: Iviglia Giorgio , Kargozar Saeid , Baino Francesco
Published: January 2019

Periodontal diseases involve injuries to the supporting structures of the tooth and, if left untreated, can lead to the loss of the tooth. Regenerative periodontal therapies aim, ideally, at healing all the damaged periodontal tissues and represent a significant clinical and societal challenge for the current ageing population. This review provides a picture of the currently-used biomaterials for periodontal regeneration, including natural and synthetic polymers, bioceramics (e.g., calcium phosphates and bioactive glasses), and composites. Bioactive materials aim at promoting the regeneration of new healthy tissue. Polymers are often used as barrier materials in guided tissue regeneration strategies and are suitable both to exclude epithelial down-growth and to allow periodontal ligament and alveolar bone cells to repopulate the defect. The problems related to the barrier postoperative collapse can be solved by using a combination of polymeric membranes and grafting materials. Advantages and drawbacks associated with the incorporation of growth factors and nanomaterials in periodontal scaffolds are also discussed, along with the development of multifunctional and multilayer implants. Tissue-engineering strategies based on functionally-graded scaffolds are expected to play an ever-increasing role in the management of periodontal defects.
Optimized Memory Allocation and Power Minimization for FPGA-Based Image Processing
Authors: Garcia Paulo , Bhowmik Deepayan , Stewart Robert , Michaelson Greg , Wallace Andrew
Published: January 2019

Memory is the biggest limiting factor to the widespread use of FPGAs for high-level image processing, which require complete frame(s) to be stored in situ. Since FPGAs have limited on-chip memory capabilities, efficient use of such resources is essential to meet performance, size and power constraints. In this paper, we investigate allocation of on-chip memory resources in order to minimize resource usage and power consumption, contributing to the realization of power-efficient high-level image processing fully contained on FPGAs. We propose methods for generating memory architectures, from both Hardware Description Languages and High Level Synthesis designs, which minimize memory usage and power consumption. Based on a formalization of on-chip memory configuration options and a power model, we demonstrate how our partitioning algorithms can outperform traditional strategies. Compared to commercial FPGA synthesis and High Level Synthesis tools, our results show that the proposed algorithms can result in up to 60% higher utilization efficiency, increasing the sizes and/or number of frames that can be accommodated, and reduce frame buffers’ dynamic power consumption by up to approximately 70%. In our experiments using Optical Flow and MeanShift Tracking, representative high-level algorithms, data show that partitioning algorithms can reduce total power by up to 25% and 30%, respectively, without impacting performance.
A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
Authors: Patel Robin
Published: January 2019

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.