bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of CO2 storage potential of oil reservoirs in Ordos Basin
Authors: Zhou Yuli , Chen Jianguo , Liu Jinhui , Yuan Hongyong
Published: July 2020

The huge impact of the emission of the greenhouse gas CO2 on the global climate and environment has caused wide concern all over the world. Deep CO2 emission reduction is the only way for sustainable development of human beings. In order to meet the urgent need of sustainable economic and social development, effective measures must be taken to achieve large-scale CO2 emission reduction. As an increasingly mature and feasible technology, CO2 geological sequestration has become one of the effective ways to achieve large-scale CO2 emission reduction. In ordos basin as the research object, this paper documents and basic information system collect and organize data, evaluated the ordos basin in turn CO2 enhanced oil recovery (CO2-EOR) and CO2 sequestration potential, depleted oil reservoir and select 60 oilfield in ordos basin CO2 sequestration potential analysis and to meet the requirements of 27 oilfield seal ratio analysis, the results showed that: Under the screening condition that the burial depth is greater than 800 m, when assuming that 60 oilfields in the ordos basin are all used for co2-eor, the CO2 sequestration potential of the reservoir is about 318.27 Mt; when 60 oilfields are all treated as abandoned reservoirs, the CO2 sequestration potential of the reservoir is about 1221.57 Mt. When the amount of CO2 to be sequestered is small, Huachi (Changqing) field should be preferred, and when the amount of CO2 to be sequestered is large, Jiyuan (Changqing) field should be preferred. In this paper, the evaluation results of CO2 sequestration potential of reservoir in ordos basin are of great significance to the further development of theoretical sequestration potential evaluation and engineering planning of ordos basin.
Understanding effects of printhead geometry in aerosol jet printing
Authors: Tafoya Rebecca R , Secor Ethan B
Published: July 2020

Aerosol jet printing offers a versatile, high-resolution digital patterning capability broadly relevant to flexible and printed electronic systems. Despite its promise and numerous demonstrations, the theoretical principles driving process outputs have not been thoroughly explored. Here a custom-built, modular printing system is developed to provide a head-to-head comparison of two print nozzle geometries to better understand the technology. Print resolution data from a range of process parameters are analyzed using a support vector machine framework. The linear deposition rate is identified as a key variable, which can confound careful studies of printing performance. Taking this into account, a clear difference is observed between the printheads, corresponding to a difference in resolution of 57% ± 11% under typical conditions. Models to understand differences in aerodynamic and mass transport effects identify enhanced drying within a large diameter printhead as a likely cause of this difference. Overall, this study provides improved understanding of the aerosol jet printing process, including valuable insight to inform process optimization, robust data analysis, ink formulation, and printer geometric design.
Interactions of different hydrocolloids with milk proteins
Authors: Hege Judith , Palberg Thomas , Vilgis Thomas A
Published: July 2020

To control rheological properties and accomplish perfect sensory properties and mouthfeel, polysaccharides are added to milk-based beverages. However, in contrast to expectations, it is often found that adding low concentrations of xanthan gum or guar gum to milk provokes phase separations of unclear physical origin. From this observation, questions arise regarding the interaction of added polysaccharides and the proteins present in milk – caseins and whey proteins. The focus of this study is to investigate such systems and to understand the basic interactions of caseins and whey proteins with different hydrocolloids. The hydrocolloids used in this study are xanthan gum, guar gum, gellan gum as well as iota-carrageenan, which were dissolved in pasteurized, non-homogenized, skimmed milk. The methods used for the examinations are light microscopy, measurement of zeta potential, atomic force microscopy and measurement of particle sizes. It was found for the case of xanthan gum dissolved in milk that the xanthan gum molecules and some of the whey proteins are found in the upper phase whereas the casein micelles as well as whey proteins are in the lower phase. For the case of guar gum dissolved in milk, the guar gum molecules are present in the upper phase and the casein micelles are present in the lower phase. This phase separation is probably caused by depletion interaction. Whey proteins are found in both phases. For the cases of iota-carrageenan, respectively, gellan gum dissolved in milk no macroscopic phase separation is observed and the measurements suggest the formation of complexes between the hydrocolloid and whey proteins.
Revisiting the coupling between accessibility and population growth
Authors: Volpati Valerio , Barthelemy Marc
Published: July 2020

The coupling between population growth and transport accessibility has been an elusive problem for more than 60 years now. Due to the lack of theoretical foundations, most of the studies that considered how the evolution of transportation networks impacts the population growth are based on regression analysis in order to identify relevant variables. The recent availability of large amounts of data allows us to envision the construction of new approaches for understanding this coupling between transport and population growth. Here, we use a detailed dataset for about 36 000 municipalities in France from 1968 until now. In the case of large urban areas such as Paris, we show that growth rate statistical variations decay in time and display a trend towards homogenization where local aspects are less relevant. We also show that growth rate differences due to accessibility are very small and can mostly be observed for cities that experienced very large accessibility variations. This suggests that the relevant variable for explaining growth rate variations is not the accessibility but its temporal variation. We propose a model that integrates the stochastic internal variation of the municipalities population and an inter-urban migration term that we show to be proportional to the accessibility variation and has a limited time duration. This model provides a simple theoretical framework that allows to go beyond econometric studies and sheds a new light on the impact of transportation modes on city growth.
A powerful framework for an integrative study with heterogeneous omics data: from univariate statistics to multi-block analysis
Authors: Duruflé Harold , Selmani Merwann , Ranocha Philippe , Jamet Elisabeth , Dunand Christophe , Déjean Sébastien
Published: July 2020

High-throughput data generated by new biotechnologies require specific and adapted statistical treatment in order to be efficiently used in biological studies. In this article, we propose a powerful framework to manage and analyse multi-omics heterogeneous data to carry out an integrative analysis. We have illustrated this using the mixOmics package for R software as it specifically addresses data integration issues. Our work also aims at applying the most recent functionalities of mixOmics to real datasets. Although multi-block integrative methodologies exist, we hope to encourage a more widespread use of such approaches in an operational framework by biologists. We have used natural populations of the model plant Arabidopsis thaliana in this work, but the framework proposed is not limited to this plant and can be deployed whatever the organisms of interest and the biological question may be. Four omics datasets (phenomics, metabolomics, cell wall proteomics and transcriptomics) were collected, analysed and integrated to study the cell wall plasticity of plants exposed to sub-optimal temperature growth conditions. The methodologies presented here start from basic univariate statistics leading to multi-block integration analysis. We have also highlighted the fact that each method, either unsupervised or supervised, is associated with one biological issue. Using this powerful framework enabled us to arrive at novel conclusions on the biological system, which would not have been possible using standard statistical approaches.
Seqminer2: an efficient tool to query and retrieve genotypes for statistical genetics analyses from biobank scale sequence dataset
Authors: Yang Lina , Jiang Shuang , Jiang Bibo , Liu Dajiang J , Zhan Xiaowei
Published: July 2020

Here, we present a highly efficient R-package seqminer2 for querying and retrieving sequence variants from biobank scale datasets of millions of individuals and hundreds of millions of genetic variants. Seqminer2 implements a novel variant-based index for querying VCF/BCF files. It improves the speed of query and retrieval by several magnitudes compared to the state-of-the-art tools based upon tabix. It also reimplements support for BGEN and PLINK format, which improves speed over alternative implementations. The improved efficiency and comprehensive support for popular file formats will facilitate method development, software prototyping and data analysis of biobank scale sequence datasets in R.

Availability and implementation

The seqminer2 R package is available from https://github.com/zhanxw/seqminer. Scripts used for the benchmarks are available in https://github.com/yang-lina/seqminer/blob/master/seqminer2%20benchmark%20script.txt.

Supplementary information

Supplementary data are available at Bioinformatics online.
The finer points of urban adaptation: intraspecific variation in lizard claw morphology
Authors: Falvey Cleo H , Aviles-Rodriguez Kevin J , Hagey Travis J , Winchell Kristin M
Published: July 2020

Human activity drastically transforms landscapes, generating novel habitats to which species must adaptively respond. Consequently, urbanization is increasingly recognized as a driver of phenotypic change. The structural environment of urban habitats presents a replicated natural experiment to examine trait–environment relationships and phenotypic variation related to locomotion. We use geometric morphometrics to examine claw morphology of five species of Anolis lizards in urban and forest habitats. We find that urban lizards undergo a shift in claw shape in the same direction but varying magnitude across species. Urban claws are overall taller, less curved, less pointed and shorter in length than those of forest lizards. These differences may enable more effective attachment or reduce interference with toepad function on smooth anthropogenic substrates. We also find an increase in shape disparity, a measurement of variation, in urban populations, suggesting relaxed selection or niche expansion rather than directional selection. This study expands our understanding of the relatively understudied trait of claw morphology and adds to a growing number of studies demonstrating phenotypic changes in urban lizards. The consistency in the direction of the shape changes we observed supports the intriguing possibility that urban environments may lead to predictable convergent adaptive change.
Back from the Ashes of Communism: The Rebirth of the Social Work Profession in Romania
Authors: Lazăr Florin , Lightfoot Elizabeth , Iovu Mihai Bogdan , Dégi László Csaba
Published: July 2020

The profession of social work in Romania was re-established in the early 1990s after being completely abolished for three decades during the communist period. This article reports findings from the first national survey of Romanian social workers, which studied characteristics of social workers and their roles, tasks and types of services provided. In addition, it explores how burnout, income and efficacy were associated with social workers’ plans for leaving the profession or country. This study used a seventy-three-item online survey tool to collect data from 1,057 social workers from across Romania using a quota sampling strategy. Romanian social workers skewed young, female and from the Romanian ethnic group. Whilst Romania has a large rural population, social workers primarily practiced in urban areas. Social workers had fairly high levels of job satisfaction and feelings of self-efficacy, and were most likely to be working in child and family protection using direct practice methods. Over one-fifth of social workers indicated they were considering leaving the field in the next two years. Those with higher incomes had lower odds that they planned to leave the field within two years, whilst those with burnout had over twice the odds they planned to leave the field.
Comparative wood anatomy ofAstropanaxandNeocussonia, an Afro-Malagasy lineage of Araliaceae
Authors: Zhernova Daiana A , Nilova Maya V , Oskolski Alexei A
Published: July 2020

Astropanax and Neocussonia are two recently resurrected genera of Araliaceae that had long been considered as an Afro-Malagasy lineage of Schefflera. The wood structure of 11 Neocussonia spp. and eight Astropanax spp. has been studied. Neocussonia shows a higher average length of vessel elements (1319 µm) and number of bars on perforation plates (up to 66) than any other Araliaceae examined to date. Neocussonia is distinct from Astropanax by its smaller diameter and higher frequency of vessels, rare occurrence of simple perforation plates, more numerous bars on scalariform perforation plates and smaller intervessel pits. The interspecific variation in percentage of simple perforation plates, bar number on scalariform perforation plates, vessel frequency and uniseriate ray number is affected by seasonality in temperature and precipitation. The sharp distinction in wood structure between samples of A. abyssinicus from Cameroon and Burundi suggests that the populations from different parts of the disjunct range represent two different species. Our data also suggest that A. goetzenii is an artificial group. Astropanax myrianthus from Madagascar and A. polysciadus from continental Africa, two cryptic species that have long been included in Schefflera myriantha, show significant differences in vessel size and grouping, percentage of simple perforation plates and intervessel pit size.
The glycocalyx core protein Glypican 1 protects vessel wall endothelial cells from stiffness-mediated dysfunction and disease
Authors: Mahmoud Marwa , Mayer Mariya , Cancel Limary M , Bartosch Anne Marie , Mathews Rick , Tarbell John M
Published: July 2020

Aims

Arterial stiffness is an underlying risk factor and a hallmark of cardiovascular diseases. The endothelial cell (EC) glycocalyx is a glycan rich surface layer that plays a key role in protecting against EC dysfunction and vascular disease. However, the mechanisms by which arterial stiffness promotes EC dysfunction and vascular disease are not fully understood, and whether the mechanism involves the protective endothelial glycocalyx is yet to be determined. We hypothesized that endothelial glycocalyx protects the endothelial cells lining the vascular wall from dysfunction and disease in response to arterial stiffness.

Methods and results

Cells cultured on polyacrylamide (PA) gels of substrate stiffness 10 kPa (mimicking the subendothelial stiffness of aged, unhealthy arteries) showed a significant inhibition of glycocalyx expression compared to cells cultured on softer PA gels (2.5 kPa, mimicking the subendothelial stiffness of young, healthy arteries). Specifically, gene and protein analyses revealed that a glycocalyx core protein Glypican 1 was inhibited in cells cultured on stiff PA gels. These cells had enhanced endothelial cell dysfunction as determined by enhanced cell inflammation (enhanced inflammatory gene expression, monocyte adhesion, and inhibited nitric oxide expression), proliferation, and EndMT. Removal of Glypican 1 using gene-specific silencing with siRNA or gene overexpression using a plasmid revealed that Glypican 1 is required to protect against stiffness-mediated endothelial cell dysfunction. Consistent with this, using a model of age-mediated stiffness, older mice exhibited a reduced expression of Glypican 1 and enhanced endothelial cell dysfunction compared to young mice. Glypican 1 gene deletion in knockout mice (GPC1−/−) exacerbated endothelial dysfunction in young mice, which normally had high endothelial expression, but not in old mice that normally expressed low levels. Endothelial cell dysfunction was exacerbated in young, but not aged, Glypican 1 knockout mice (GPC1−/−).

Conclusion

Arterial stiffness promotes EC dysfunction and vascular disease at least partly through the suppression of the glycocalyx protein Glypican 1. Glypican 1 contributes to the protection against endothelial cell dysfunction and vascular disease in endothelial cells.
Spotted Lanternfly (Hemiptera: Fulgoridae) Can Complete Development and Reproduce Without Access to the Preferred Host, Ailanthus altissima
Authors: Uyi Osariyekemwen , Keller Joseph A , Johnson Anne , Long David , Walsh Brian , Hoover Kelli
Published: July 2020

Despite its broad host range, the spotted lanternfly Lycorma delicatula (White), is known to have a marked preference for Ailanthus altissima. However, whether this polyphagous phloem feeder can complete its life cycle in the absence of A. altissima is unknown. We examined the performance of L. delicatula with and without access to A. altissima by tracking development, survival, host tree species association, and oviposition in large enclosures planted with Salix babylonica and Acer saccharinum along with either A. altissima or Betula nigra. We monitored enclosures from late May 2019 through June 2020. Lycorma delicatula survival was slightly higher in enclosures with A. altissima and 50% of individuals in A. altissima enclosures reached the adult stage ~6.5 d earlier than in enclosures without A. altissima. In the presence of A. altissima, nymphs were most frequently observed on this host while adults were found at similar frequencies on A. altissima and A. saccharinum. In the absence of A. altissima, nymphs were most frequently associated with S. babylonica and A. saccharinum, while adults were most often found on A. saccharinum. Females laid a total of 46 and 6 egg masses in enclosures with and without A. altissima, respectively, before freezing temperatures killed the remaining adults. The proportion of eggs that hatched per egg mass did not differ between treatments. Although L. delicatula can complete development and reproduce on other host species without access to A. altissima, fitness was reduced. These findings have implications for management that relies exclusively on treatment of A. altissima.
SARS, MERS and COVID-19: clinical manifestations and organ-system complications: a mini review
Authors: Gerges Harb Jad , Noureldine Hussein A , Chedid Georges , Eldine Mariam Nour , Abdallah Dany Abou , Chedid Nancy Falco , Nour-Eldine Wared
Published: July 2020

Middle East Respiratory Syndrome (MERS), Severe Acute Respiratory Syndrome (SARS) and Coronavirus Disease 2019 (COVID-19) are caused by three distinct coronaviruses belonging to the same genus. COVID-19 and its two predecessors share many important features in their clinical presentations, and in their propensity for progression to severe disease which is marked by high rates of morbidity and mortality. However, comparison of the three viral illnesses also reveals a number of specific differences in clinical manifestations and complications, which suggest variability in the disease process. This narrative review delineates the pulmonary, cardiac, renal, gastrointestinal, hepatic, neurological and hematologic complications associated with these three respiratory coronaviruses. It further describes the mechanisms of immune hyperactivation—particularly cytokine release syndrome—implicated in the multi-organ system injury seen in severe cases of MERS, SARS and COVID-19.
Motor neuron translatome reveals deregulation of SYNGR4 and PLEKHB1 in mutant TDP-43 amyotrophic lateral sclerosis models
Authors: Marques Rita F , Engler Jan B , Küchler Katrin , Jones Ross A , Lingner Thomas , Salinas Gabriela , Gillingwater Thomas H , Friese Manuel A , Duncan Kent E
Published: July 2020

Amyotrophic lateral sclerosis (ALS) is an incurable neurological disease with progressive loss of motor neuron (MN) function in the brain and spinal cord. Mutations in TARDBP, encoding the RNA-binding protein TDP-43, are one cause of ALS, and TDP-43 mislocalization in MNs is a key pathological feature of >95% of ALS cases. While numerous studies support altered RNA regulation by TDP-43 as a major cause of disease, specific changes within MNs that trigger disease onset remain unclear. Here, we combined translating ribosome affinity purification (TRAP) with RNA sequencing to identify molecular changes in spinal MNs of TDP-43–driven ALS at motor symptom onset. By comparing the MN translatome of hTDP-43A315T mice to littermate controls and to mice expressing wild type hTDP-43, we identified hundreds of mRNAs that were selectively up- or downregulated in MNs. We validated the deregulated candidates Tex26, Syngr4, and Plekhb1 mRNAs in an independent TRAP experiment. Moreover, by quantitative immunostaining of spinal cord MNs, we found corresponding protein level changes for SYNGR4 and PLEKHB1. We also observed these changes in spinal MNs of an independent ALS mouse model caused by a different patient mutant allele of TDP-43, suggesting that they are general features of TDP-43-driven ALS. Thus, we identified SYNGR4 and PLEKHB1 to be deregulated in MNs at motor symptom onset in TDP-43-driven ALS models. This spatial and temporal pattern suggests that these proteins could be functionally important for driving the transition to the symptomatic phase of the disease.
Risk factors for positive and negative COVID-19 tests: a cautious and in-depth analysis of UK biobank data
Authors: Chadeau-Hyam Marc , Bodinier Barbara , Elliott Joshua , Whitaker Matthew D , Tzoulaki Ioanna , Vermeulen Roel , Kelly-Irving Michelle , Delpierre Cyrille , Elliott Paul
Published: July 2020

Background

The recent COVID-19 outbreak has generated an unprecedented public health crisis, with millions of infections and hundreds of thousands of deaths worldwide. Using hospital-based or mortality data, several COVID-19 risk factors have been identified, but these may be confounded or biased.

Methods

Using SARS-CoV-2 infection test data (n = 4509 tests; 1325 positive) from Public Health England, linked to the UK Biobank study, we explored the contribution of demographic, social, health risk, medical and environmental factors to COVID-19 risk. We used multivariable and penalized logistic regression models for the risk of (i) being tested, (ii) testing positive/negative in the study population and, adopting a test negative design, (iii) the risk of testing positive within the tested population.

Results

In the fully adjusted model, variables independently associated with the risk of being tested for COVID-19 with odds ratio >1.05 were: male sex; Black ethnicity; social disadvantage (as measured by education, housing and income); occupation (healthcare worker, retired, unemployed); ever smoker; severely obese; comorbidities; and greater exposure to particulate matter (PM) 2.5 absorbance. Of these, only male sex, non-White ethnicity and lower educational attainment, and none of the comorbidities or health risk factors, were associated with testing positive among tested individuals.

Conclusions

We adopted a careful and exhaustive approach within a large population-based cohort, which enabled us to triangulate evidence linking male sex, lower educational attainment and non-White ethnicity with the risk of COVID-19. The elucidation of the joint and independent effects of these factors is a high-priority area for further research to inform on the natural history of COVID-19.
Imagined Insecurities in Imagined Communities: Manufacturing the Ethnoreligious Others as Security Threats
Authors: Magcamit Michael Intal
Published: July 2020

How does a once familiar and benign ethnoreligious community become a stranger and a threat? This article examines the underlying causal mechanisms driving rival ethnoreligious factions within pluralistic polities to frame each other as threats to their relative security, power, and status. Drawing on complementary theories from critical security, religious, and nationalism studies, I develop a framework that captures and explains the processes and dynamics through which threatening conceptions and narratives about the ethnoreligious others are constructed, socialized, and legitimized over time. To theoretically probe and empirically demonstrate the utility of this framework, I examine how the collective imagined insecurities among Muslim and Christian communities in Indonesia have crystallized into tangible security threats using the interpretive process tracing method. Evidences produced from my theoretical and empirical analyses using the novel qualitative data I gathered from my field research reveal that this chauvinistic, zero-sum phenomenon proceeds via a three-phase othering causal mechanism comprised of cultivation of hostile emotive effects of ethnoreligious nationalism, securitization of othered ethnoreligious groups using hostile symbolic predispositions, and sacralization of hostile perceptions of indivisible ethnoreligious identities and homelands.
Evaluation of physical euthanasia for neonatal piglets on-farm
Authors: Dalla Costa Filipe Antonio , Gibson Troy J , Oliveira Steffan Edward Octávio , Gregory Neville George , Coldebella Arlei , Faucitano Luigi , Ludtke Charli Beatriz , Buss Liziè Peréirã , Dalla Costa Osmar Antonio
Published: July 2020

Twenty-seven neonate piglets (range from 0.35 to 1.17 kg) were evaluated for the effectiveness of blunt force trauma as a method of on-farm cull. Brainstem function, brain injury, and hemorrhage scores (increasing from 0 to 3) were assessed after striking the head against a concrete floor. Electroencephalograms (EEG) from a subset of 15 piglets were recorded before and after blunt force trauma for electrophysiological assessments. Blunt force trauma was performed by a single experienced farmer in a commercial farm by holding the piglet by its both hind legs and striking the head against the concrete floor. All piglets remained recumbent and did not show brainstem reflexes. Only one piglet did not presented tonic/clonic physical activity. The mean time to the onset of persistent isoelectric EEG was 64.3 ± 7.3 s (range 18 to 115). Total power, theta, alpha, and beta power decreased to approximately 45%, 30%, 20%, and 15% from pretreatment power, respectively, by 15-s post-impact. There were no periods of normal-like EEG after the culling. Bruises in the neck and shoulder were found in 67% and 70% of piglets, respectively. All piglets presented skull fractures with 20% having the nasal bone(s) fractured. Brain damage was found in all piglets, mainly in the frontal lobe(s). The occipital lobe(s) presented the greatest frequency of severe damage. The analysis of the radiographs also found a high frequency of fractures in this region. Hemorrhage was most frequent in the frontal, parietal, occipital lobes, and midbrain. When performed correctly with the appropriate weight class, blunt force trauma can be used as an effective method for the on-farm killing of nursing piglets resulting in death. However, this method should not be promoted over more reliable and repeatable cull methods such as captive bolt gun. As with blunt force trauma, there is a significant potential for animal welfare harm associated with inappropriate practice, lack of accuracy, issues with repeatability, and operator fatigue.
Reproductive Traits of Ontsira mellipes (Hymenoptera: Braconidae), a North American Parasitoid, as a Novel Biological Control Agent for Exotic Anoplophora glabripennis (Coleoptera: Cerambycidae)
Authors: Wang Xingeng , Aparicio Ellen M
Published: July 2020

Ontsira mellipes Ashmead is a gregarious larval ectoparasitoid of woodboring cerambycids that is native to North America but can readily attack the exotic Asian longhorned beetle, Anoplophora glabripennis (Motschulsky). To evaluate the potential of the parasitoid as a novel association control agent for the pest beetle, this study investigated some key reproductive traits of the parasitoid, including egg maturation dynamics, and host size preference and suitability in association with the beetle. Results showed that female wasps emerged with a substantial portion (38%) of their lifetime complement of mature eggs and matured eggs rapidly, reaching a peak 4–6 d post-eclosion. The number of mature eggs was positively related to the female wasp’s body size. Oviposition prompted production of more mature eggs by young female wasps. The parasitoid did not show a significant preference for large over small hosts in a choice test. Host size did not affect the parasitoid’s offspring survival, developmental time, or sex ratio. However, clutch size increased with increasing host size. Female wasps that developed from large hosts had larger body size and consequently a higher mature egg load than those reared from small hosts. Neither longevity nor the total number of parasitized hosts over a female’s lifetime was affected by the female’s size, but the total number of offspring produced per female increased with the female’s size. These results have important implications for improving rearing and field-release strategies as well as understanding the ecological mechanisms underlying host size selection in gregarious parasitoids.
Delivery Methods for RNAi in Mosquito Larvae
Authors: Munawar Kashif , Alahmed Azzam M , Khalil Sayed M S
Published: July 2020

Mosquito-transmitted diseases pose a threat for a great portion of the world population. Chemical insecticides are the main tool for mosquito control. Heavy dependence on chemicals created several problems such as resistance development in many mosquito species, environmental effects, and human health issues. Other tools for mosquito control were developed and used in some parts of the world. Ribonucleic acid interference (RNAi) is a reverse genetic mechanism that was recently introduced as a new tool for pest control. Regarding mosquito, RNAi was used to study gene function and to discover genes that can be used as targets for control purposes. Several delivery methods are used to induce RNAi in mosquito larvae. Some methods such as injection and soaking are used routinely in RNAi research but have no application in the field. Other methods such as nanoparticles and microbes have some characteristics that make them good candidates for field application. In this report, we will focus on delivery methods for RNAi in mosquito larvae and will give examples for each method.
Gastric outlet obstruction secondary to peptic ulcer disease, previously misdiagnosed as idiopathic hypertrophic pyloric stenosis in a 16-year-old girl: a case report
Authors: Notue Yves Alain , Mbessoh Ulrich Igor , Tientcheu Tim Fabrice , Moifo Boniface , Mefire Alain Chichom
Published: July 2020

Gastric outlet obstruction encompasses a broad spectrum of conditions characterized by complete or incomplete obstruction of the distal stomach, which interrupts gastric emptying and prevents the passage of gastric contents beyond the proximal duodenum. Idiopathic hypertrophic pyloric stenosis is the most common cause with an incidence of 1.5–3 per 1000 live births. However, it is excluded; other causes in children such as peptic ulcer disease are relatively rare. We report a case of an acquired gastric outlet obstruction due to peptic ulcer disease, previously misdiagnosed as idiopathic hypertrophic pyloric stenosis in a 16-year-old girl. Beyond the rarity of this clinical event, this case highlights the challenges of the aetiological diagnosis of gastric outlet obstruction with subsequent therapeutic issues, and is the first documented case in Cameroon.
A Case Report: Insulinoma in a Military Pilot Detected by 68Ga-Dotatate PET/CT
Authors: Larose Lisa D , Vroman Penny J , Musick Sierra R , Beauvais Alexis A
Published: July 2020

A 37-year-old active duty male Air Force instructor pilot, with no prior medical history, was found unresponsive at his home after awakening with symptoms of altered mental status when the Emergency Medical Service (EMS) was called. The patient was found to be hypoglycemic with a glucose of 37 mg/dL. The patient recovered after administration of a dextrose bolus. Further investigation revealed that over the last several years, the patient exhibited symptoms of lightheadedness and tremors if fasted greater than 3 hours. Further clinical workup strongly suggested the presence of a neuroendocrine tumor. Initial imaging studies to include a multiphasic dedicated pancreatic computed tomography (CT) scan did not demonstrate a pancreatic lesion. However, the utilization of an innovative new nuclear medicine imaging modality, a 68Ga-Dotatate PET/CT, clearly demonstrated a 19 × 16 mm lesion of the distal pancreatic tail, which guided surgical resection. He underwent a robotic-assisted laparoscopic distal pancreatectomy, pathologically characterized as an insulinoma. The patient’s symptoms immediately resolved with no recurrence over the next 6 months. The pilot was granted a waiver, returning him to his flying duties. The 68Ga-Dotatate PET/CT enabled the identification of an otherwise occult pancreatic neuroendocrine tumor ultimately leading to this patient’s definitive cure and the salvage of this military asset’s aviation career.
petar: a high-performance N-body code for modelling massive collisional stellar systems
Authors: Wang Long , Iwasawa Masaki , Nitadori Keigo , Makino Junichiro
Published: July 2020

The numerical simulations of massive collisional stellar systems, such as globular clusters (GCs), are very time consuming. Until now, only a few realistic million-body simulations of GCs with a small fraction of binaries ($5{{\ \rm per\ cent}}$) have been performed by using the nbody6++gpu code. Such models took half a year computational time on a Graphic Processing Unit (GPU)-based supercomputer. In this work, we develop a new N-body code, petar, by combining the methods of Barnes–Hut tree, Hermite integrator and slow-down algorithmic regularization. The code can accurately handle an arbitrary fraction of multiple systems (e.g. binaries and triples) while keeping a high performance by using the hybrid parallelization methods with mpi, openmp, simd instructions and GPU. A few benchmarks indicate that petar and nbody6++gpu have a very good agreement on the long-term evolution of the global structure, binary orbits and escapers. On a highly configured GPU desktop computer, the performance of a million-body simulation with all stars in binaries by using petar is 11 times faster than that of nbody6++gpu. Moreover, on the Cray XC50 supercomputer, petar well scales when number of cores increase. The 10 million-body problem, which covers the region of ultracompact dwarfs and nuclear star clusters, becomes possible to be solved.
SN 2018hti: a nearby superluminous supernova discovered in a metal-poor galaxy
Authors: Lin W L , Wang X F , Li W X , Zhang J J , Mo J , Sai H N , Zhang X H , Filippenko A V , Zheng W K , Brink T G , Baron E , DerKacy J M , Ehgamberdiev S A , Mirzaqulov D , Li X , Zhang J C , Yan S Y , Xi G B , Hsiao Y , Zhang T M , Wang L J , Liu L D , Xiang D F , Wu C Y , Rui L M , Chen Z H
Published: July 2020

SN 2018hti is a Type I superluminous supernova (SLSN I) with an absolute g-band magnitude of −22.2 at maximum brightness, discovered by the Asteroid Terrestrial-impact Last Alert System in a metal-poor galaxy at a redshift of 0.0612. We present extensive photometric and spectroscopic observations of this supernova, covering the phases from ∼−35 d to more than  +340 d from the r-band maximum. Combining our BVgri-band photometry with Swift UVOT optical/ultraviolet photometry, we calculated the peak luminosity as ∼3.5 × 1044 erg s−1. Modelling the observed light curve reveals that the luminosity evolution of SN 2018hti can be produced by an ejecta mass of 5.8 M⊙ and a magnetar with a magnetic field of B = 1.8 × 1013 G having an initial spin period of P0 = 1.8 ms. Based on such a magnetar-powered scenario and a larger sample, a correlation between the spin of the magnetar and the kinetic energy of the ejecta can be inferred for most SLSNe I, suggesting a self-consistent scenario. Like for other SLSNe I, the host galaxy of SN 2018hti is found to be relatively faint (Mg = −17.75 mag) and of low metallicity (Z = 0.3 Z⊙), with a star formation rate of 0.3 M⊙ yr−1. According to simulation results of single-star evolution, SN 2018hti could originate from a massive, metal-poor star with a zero-age main sequence (ZAMS) mass of 25–40 M⊙, or from a less massive rotating star with MZAMS ≈ 16–25 M⊙. For the case of a binary system, its progenitor could also be a star with $M_\mathrm{ZAMS} \gtrsim 25\, \mathrm{ M}_\odot$.
Implementation of stellar heating feedback in simulations of star cluster formation: effects on the initial mass function
Authors: Mathew Sajay Sunny , Federrath Christoph
Published: July 2020

Explaining the initial mass function (IMF) of stars is a long-standing problem in astrophysics. The number of complex mechanisms involved in the process of star cluster formation, such as turbulence, magnetic fields, and stellar feedback, make understanding and modelling the IMF a challenging task. In this paper, we aim to assert the importance of stellar heating feedback in the star cluster formation process and its effect on the shape of the IMF. We use an analytical sub-grid model to implement the radiative feedback in fully three-dimensional magnetohydrodynamical (MHD) simulations of star cluster formation, with the ultimate objective of obtaining numerical convergence on the IMF. We compare a set of MHD adaptive mesh refinement simulations with three different implementations of the heating of the gas: (1) a polytropic equation of state, (2) a spherically symmetric stellar heating feedback, and (3) our newly developed polar heating model that takes into account the geometry of the accretion disc and the resulting shielding of stellar radiation by dust. For each of the three heating models, we analyse the distribution of stellar masses formed in 10 molecular cloud simulations with different realizations of the turbulence to obtain a statistically representative IMF. We conclude that stellar heating feedback has a profound influence on the number of stars formed and plays a crucial role in controlling the IMF. We find that the simulations with the polar heating model achieve the best convergence on the observed IMF.
The failure of testing for cosmic opacity via the distance-duality relation
Authors: Vavryčuk Václav , Kroupa Pavel
Published: July 2020

The distance-duality relation (DDR) between the luminosity distance DL and the angular diameter distance DA is viewed as a powerful tool for testing for the opacity of the Universe, being independent of any cosmological model. It was applied by many authors, who mostly confirm its validity and report a negligible opacity of the Universe. Nevertheless, a thorough analysis reveals that applying the DDR in cosmic opacity tests is tricky. Its applicability is strongly limited because of a non-unique interpretation of the DL data in terms of cosmic opacity and a rather low accuracy and deficient extent of currently available DA data. Moreover, authors usually assume that cosmic opacity is frequency independent and parametrize it in their tests by a prescribed phenomenological function. In this way, they only prove that cosmic opacity does not follow their assumptions. As a consequence, no convincing evidence of transparency of the universe using the DDR has so far been presented.
Warm dust in high-z galaxies: origin and implications
Authors: Sommovigo L , Ferrara A , Pallottini A , Carniani S , Gallerani S , Decataldo D
Published: July 2020

ALMA observations have revealed the presence of dust in galaxies in the Epoch of Reionization (EoR; redshift z > 6). However, the dust temperature, Td, remains unconstrained, and this introduces large uncertainties, particularly in the dust mass determinations. Using an analytical and physically motivated model, we show that dust in high-z, star-forming giant molecular clouds (GMCs), largely dominating the observed far-infrared luminosity, is warmer ($T_\mathrm{ d} \lower.5ex\hbox{$\,\, \buildrel\,\gt\, \over \sim \,\,$}60\ \mathrm{K}$) than locally. This is due to the more compact GMC structure induced by the higher gas pressure and turbulence characterizing early galaxies. The compactness also delays GMC dispersal by stellar feedback, thus $\sim 40$ per cent of the total UV radiation emitted by newly born stars remains obscured. A higher Td has additional implications: it (a) reduces the tension between local and high-z IRX–β relation, and (b) alleviates the problem of the uncomfortably large dust masses deduced from observations of some EoR galaxies.
Chemical models of interstellar cyanomethanimine isomers
Authors: Zhang Xia , Quan Donghui , Chang Qiang , Herbst Eric , Esimbek Jarken , Webb Matthew
Published: July 2020

The E-isomer of cyanomethanimine (HNCHCN) was first identified in Sagittarius B2(N) (Sgr B2(N)) by a comparison of the publicly available Green Bank Telescope (GBT) PRIMOS survey with laboratory rotational spectra. Recently, Z-cyanomethanimine was detected in the quiescent molecular cloud G+0.693−0.027 with the IRAM 30-m telescope. Cyanomethanimine is a chemical intermediate in the proposed synthetic routes of adenine, and may play an important role in forming biological molecules in the interstellar medium. Here we present a new modelling study of cyanomethanimine, using the nautilus gas–grain reaction network and code with the addition of over 400 chemical reactions of the three cyanomethanimine isomers and related species. We apply cold isothermal core, hot core, and C-type shock models to simulate the complicated and heterogeneous physical environment in and in front of Sgr B2(N), and in G+0.693−0.027. We identify the major formation and destruction routes of cyanomethanimine, and find that the calculated abundances of the cyanomethanimine isomers and the ratio of Z-isomer to E-isomer are both in reasonable agreement with observations for selected environments. In particular, we conclude that these isomers are most likely formed within or near the hot core without the impact of shocks, or in the cold regions with shocks.
Structures of mammalian GLD-2 proteins reveal molecular basis of their functional diversity in mRNA and microRNA processing
Authors: Ma Xiao-Yan , Zhang Hong , Feng Jian-Xiong , Hu Jia-Li , Yu Bing , Luo Li , Cao Yu-Lu , Liao Shuang , Wang Jichang , Gao Song
Published: July 2020

The stability and processing of cellular RNA transcripts are efficiently controlled via non-templated addition of single or multiple nucleotides, which is catalyzed by various nucleotidyltransferases including poly(A) polymerases (PAPs). Germline development defective 2 (GLD-2) is among the first reported cytoplasmic non-canonical PAPs that promotes the translation of germline-specific mRNAs by extending their short poly(A) tails in metazoan, such as Caenorhabditis elegans and Xenopus. On the other hand, the function of mammalian GLD-2 seems more diverse, which includes monoadenylation of certain microRNAs. To understand the structural basis that underlies the difference between mammalian and non-mammalian GLD-2 proteins, we determine crystal structures of two rodent GLD-2s. Different from C. elegans GLD-2, mammalian GLD-2 is an intrinsically robust PAP with an extensively positively charged surface. Rodent and C. elegans GLD-2s have a topological difference in the β-sheet region of the central domain. Whereas C. elegans GLD-2 prefers adenosine-rich RNA substrates, mammalian GLD-2 can work on RNA oligos with various sequences. Coincident with its activity on microRNAs, mammalian GLD-2 structurally resembles the mRNA and miRNA processor terminal uridylyltransferase 7 (TUT7). Our study reveals how GLD-2 structurally evolves to a more versatile nucleotidyltransferase, and provides important clues in understanding its biological function in mammals.
Dnmt1 binds and represses genomic retroelements via DNA methylation in mouse early embryos
Authors: Min Byungkuk , Park Jung Sun , Jeong Young Sun , Jeon Kyuheum , Kang Yong-Kook
Published: July 2020

Genome-wide passive DNA demethylation in cleavage-stage mouse embryos is related to the cytoplasmic localization of the maintenance methyltransferase DNMT1. However, recent studies provided evidences of the nuclear localization of DNMT1 and its contribution to the maintenance of methylation levels of imprinted regions and other genomic loci in early embryos. Using the DNA adenine methylase identification method, we identified Dnmt1-binding regions in four- and eight-cell embryos. The unbiased distribution of Dnmt1 peaks in the genic regions (promoters and CpG islands) as well as the absence of a correlation between the Dnmt1 peaks and the expression levels of the peak-associated genes refutes the active participation of Dnmt1 in the transcriptional regulation of genes in the early developmental period. Instead, Dnmt1 was found to associate with genomic retroelements in a greatly biased fashion, particularly with the LINE1 (long interspersed nuclear elements) and ERVK (endogenous retrovirus type K) sequences. Transcriptomic analysis revealed that the transcripts of the Dnmt1-enriched retroelements were overrepresented in Dnmt1 knockdown embryos. Finally, methyl-CpG-binding domain sequencing proved that the Dnmt1-enriched retroelements, which were densely methylated in wild-type embryos, became demethylated in the Dnmt1-depleted embryos. Our results indicate that Dnmt1 is involved in the repression of retroelements through DNA methylation in early mouse development.
Probing of coupling effect induced plasmonic charge accumulation for water oxidation
Authors: Gao Yuying , Cheng Feng , Fang Weina , Liu Xiaoguo , Wang Shengyang , Nie Wei , Chen Ruotian , Ye Sheng , Zhu Jian , An Hongyu , Fan Chunhai , Fan Fengtao , Li Can
Published: July 2020

A key issue for redox reactions in plasmon-induced photocatalysis, particularly for water oxidation, is the concentration of surface-accumulating charges (electrons or holes) at a reaction site for artificial photosynthesis. However, where plasmonic charge accumulated at a catalyst's surface, and how to improve local charge density at active sites, remains unknown because it is difficult to identify the exact spatial location and local density of the plasmon-induced charge, particularly with regard to holes. Herein, we show that at the single particle level, plasmon-coupling-induced holes can be greatly accumulated at the plasmonic Au nanoparticle dimer/TiO2 interface in the nanogap region, as directly evidenced by the locally enhanced surface photovoltage. Such an accumulation of plasmonic holes can significantly accelerate the water oxidation reaction (multi-holes involved) at the interfacial reaction site, with nearly one order of magnitude enhancement in photocatalytic activities compared to those of highly dispersed Au nanoparticles on TiO2. Combining Kelvin probe force microscopy and theoretical simulation, we further clarified that the local accumulated hole density is proportional to the square of the local near-field enhancement. Our findings advance the understanding of how charges spatially distribute in plasmonic systems and the specific role that local charge density at reaction sites plays in plasmonic photocatalysis.